bluuebunny/crossref_metadata_2025 huggingface dataset:
Influência do programa PMAQ na qualificação da Atenção Básica: revisão de literatura
Authors: Lago Vivian Conceição Alves L. Pereira do
Published: August 2022

Objetivo: compreender a respeito da influência do programa PMAQ na qualificação da Atenção Básica. Metodologia: Este estudo diz respeito a uma revisão bibliográfica, de cunho integrativo, sendo os dados somados de maneira qualitativa. A amostragem foi levantada para dar suporte a este trabalho de revisão, tendo como foco a literatura acadêmica especializada, tendo como bases os dados da BVS, SCIELO e LILACS que são as principais ferramentas de pesquisa de artigo de significância literária. Resultados: A principio no filtro geral da temática foram encontrados 214 artigos que abordaram sobre o tema atenção básica com base no PMAQ-AB, após filtro sistemático restaram 50 publicações encontradas que abordaram a temática proposta, sendo que 07 artigos foram selecionados para discussão. Após leitura exploratória e crítica, os artigos foram organizados por título, periódico, autor, ano e objetivo. Considerações finais: É evidenciado neste estudo os resultados do PMAQ para melhoria do acesso a saúde na maioria das pesquisas analisadas, demonstrando que este programa é capaz de provocar nos profissionais e gestores mais necessidade de trabalhar com ações ainda mais qualificadas e que possibilite que as politicas públicas em saúde sejam cumpridas e ampliadas para atender as necessidades da população, apontando que este incentivo provoca influencia no cotidiano da saúde.
Influência da espiritualidade/religiosidade na qualidade de vida relacionada à saúde em pacientes com diabetes: revisão integrativa da literatura
Authors: Faustino Laurita , Anjos Achaiane Camila Silva dos , Somensi Lincon Bordignon , Adami Eliana Rezende
Published: August 2022

Existe uma associação entre espiritualidade e enfrentamento da doença médica crônica, vontade de viver, redução da ansiedade, da depressão e melhoria da qualidade de vida. Por intermédio do presente trabalho de revisão pretende-se avaliar artigos que abordem a espiritualidade/religiosidade na qualidade de vida de pacientes diabéticos.  Isto, porque percebe-se que há diversas estratégias utilizadas para o enfrentamento de doenças com evidências para a religião e a espiritualidade. Objetivo – Compreender a interferência e o papel da espiritualidade e religiosidade na qualidade de vida e as mudanças de comportamento de pacientes diabéticos como consequência de seu envolvimento. Metodologia - Esta revisão integrativa foi baseada nas técnicas bibliográfica, quantitativa, qualitativa, retrospectiva e transversal conforme dados da Scielo, Medline, Lilacs e PubMed com utilização dos termos descritivos: “spirituality”, “quality”, “quality of life” e “diabetic”. Foram consultados diversos artigos entre 09 de abril de 2022 e 10 de julho de 2022. Resultados – Após criteriosa análise dos artigos em questão evidenciou-se que a qualidade de vida de diabéticos é menor que a de pacientes não diabéticos. Isto ocorre devido à tristeza, ansiedade, preocupação e ao medo com seu futuro, principalmente, face à mudança do estilo de vida que a doença lhes impõe. Esses pacientes têm que lidar com alterações físicas e conflitos mentais estando sujeitos a prejuízo em seu modo de vida. Tudo isso os impele à busca da Espiritualidade.
Aspectos cirúrgicos no tratamento da Síndrome de Eagle
Authors: Pereira Mariana Helena Trinta , Diniz Nicole Serra , Nascimento Wendy Saureana Maior de Oliveira , Trinta Luana Barbieri , Flor Lara Carolyne de Sousa , Sousa Anna Carolina Azevedo , Santos Bruno Nascimento , Oliveira Luis Gustavo Soares Lula de , Nepomuceno Ana Karla Torres dos Santos , Cardoso Gabriela Dias
Published: August 2022

O processo estiloide é uma estrutura óssea que se projeta para baixo e para frente da face, estando em posição anterior ao osso Temporal, e com característica delgada e pontuda. A Síndrome de Eagle ocorre em função ou o alongamento do processo estilóide, posteriormente a uma ossificação de um remanescente embriológico da cartilagem do segundo arco branquial, ou devido à calcificação do ligamento estilo-hiódeo. O presente trabalho tem como objetivo discorrer sobre as possíveis formas de tratamento cirúrgico para a Síndrome de Eagle, e trata-se de uma revisão de literatura realizada a partir de publicações científicas em bases de dados como a Biblioteca Virtual em Saúde (BVS), Pubmed/Medline, Scielo e Google Acadêmico, utilizando descritores em nas línguas portuguesa e inglesa. Para o tratamento da Síndrome de Eagle, é considerada a severidade dos sintomas, assim, em casos de dor leve a moderada, o tratamento inicial é o tratamento farmacológico, com administração de antidepressivos, benzodiazepínicos, anticonvulsivantes ou realização de infiltrações de esteroides e/ou anestésicos locais. Entretanto, nos casos de dor intensa e severa, o tratamento proposto é a ressecção cirúrgica completa do processo estiloide (estiloidectomia), que pode ser realizada pela via intrabucal ou extrabucal, na qual a escolha de uma das duas estará baseada na experiência do cirurgião e as condições de trabalho. Dessa forma, conclui-se que a escolha do tratamento depende da avaliação minuciosa feita pelo cirurgião-dentista e pelo quadro do paciente.
Corticosteroides e anti-inflamatório não esteróide na cirurgia oral: indicação de uso
Authors: Rabelo Rannya Bernardes , Gomes Ana Viviam Souza Ferro
Published: August 2022

Os corticosteroides e os anti-inflamatórios não esteroidais são bastante prescritos para realização de cirurgias orais, contudo cada um com sua função. Assim sendo, este estudo tem como objetivo comparar essas duas classes de medicamentos, observando onde melhor se aplica cada, suas indicações e seus efeitos diante da cirurgia oral. Este artigo refere-se a uma revisão de literatura narrativa, os artigos usados como referência foram encontrados através de descritores pesquisados no PubMed, Google Acadêmico, SciELO e Portal BVS, no qual de todos artigos pesquisados foram incluídos 21 artigos para a confecção do mesmo. A literatura descreve que o uso de corticoides é recomendado de forma preemptiva para prevenir edema, trismo e dor, contudo o anti-inflamatório não esteroide é utilizado no pós-operatório para obter analgesia, também levando em consideração que o uso combinado das duas classes de medicamentos mostrou eficácia para tratamento preventivo da dor aguda no pós-operatório. Dessa forma, pode-se observar neste artigo que a classe de corticosteroide apresentou ser melhor para uso no pré-operatório, pois ocorre diminuição de edema, trismo e dor, no entanto pode concluir que o anti-inflamatório não esteroide é favorável sua utilização no pós-operatório para diminuir ou eliminar a dor que paciente possa sentir após a cirurgia.
Glifosato e o uso de pesticidas em Mato Grosso do Sul: riscos relacionados aos recursos hídricos
Authors: Garcia Luciana Virgili Pedroso , Oliveira Ademir Kleber Morbeck de , Matias Rosemary
Published: August 2022

Mato Grosso do Sul possui uma economia voltada para o agronegócio, ocupando o 9º lugar entre os estados agrícolas do país. Os impactos decorrentes do crescimento desta atividade representam um risco para as áreas úmidas. Deste modo objetivou-se realizar um levantamento dos pesticidas mais utilizados e os problemas relacionados ao produto mais vendido, o glifosato. Foi realizada uma pesquisa bibliográfica integrativa nas bases de dados Anvisa, BIREME, IBAMA, LILACS, MEDLINE, Scielo, Scopus e TOXLINE, usando diferentes combinações de termos referente à temática. Dos dez pesticidas mais utilizados, em primeiro lugar está o herbicida glifosato, que quando associado aos adjuvantes, penetra com mais facilidade nas membranas celulares das plantas daninhas, promovendo sua eliminação. Os compostos resultantes de sua degradação e os adjuvantes utilizados trazem riscos ambientais, devido seus efeitos duradouros e à dispersão ambiental, transformando-se em uma ameaça, principalmente aos corpos hídricos, receptáculo natural dos produtos utilizados. Monitorar o nível de contaminação nas águas superficiais é uma necessidade, permitindo construir um espectro dos riscos relacionados à atividade agrícola e os possíveis impactos ambientais na cadeia trófica dos ambientes aquáticos.
Mortalidade materna por infecção puerperal no estado do Piauí: um estudo epidemiológico
Authors: Sousa Gabryela Karyny Oliveira e , Crizóstomo Cilene Delgado , Alencar Larissa Nunes de , Andrade Izane Luiza Xavier Carvalho , Costa Lucas Manoel Oliveira , Andrade Aline Maria Lima , Feitosa Gaubeline Teixeira , Sousa Jéssica Lima , Silva Tatiane Sousa da
Published: August 2022

Objetivo: Ponderar acerca do perfil epidemiológico da mortalidade materna por infecção puerperal no estado de Piauí. Métodos: Trata-se de um estudo epidemiológico, retrospectivo, descritivo de caráter quantitativo dos óbitos maternos por infecção puerperal entre 2016 e 2020, os dados foram extraídos por meio do SIM e SINASC inseridos no DATASUS. Os dados foram organizados por meio de gráficos e tabelas para melhor observação e compreensão. As informações foram comparadas com a literatura científica existente acerca da temática. Resultados: Pode-se observar que o ano de 2019 apresentou maior razão de mortalidade materna por infecção puerperal em comparação aos demais anos analisados. O município que apresentou maior Razão da mortalidade materna (RMM) foi Francisco Ayres no ano de 2020. Mulheres com faixa-etária entre 20 a 29 anos, solteiras, com 4 a 7 anos de escolaridade apresentaram maior prevalência de óbito materno por infecção puerperal. Conclusão: Para mitigação das infecções é primordial que se estabeleça medidas profiláticas como a higienização apropriada de todos os processos bem como dos locais envolvidos no cuidado direto e indireto das puérperas.
Terapia comunitária: o impacto das práticas integrativas e complementares no processo saúde-doença
Authors: Bandeira Glaziane da Silva Paiva , Paiva Maria Beatriz Figueiredo , Silva Milena Pereira Patrício da , Sales Ângela Maria Geraldo , Daniele Thiago Medeiros da Costa , Bezerra Mariana Vieira de Melo , Bizerril Davi Oliveira
Published: August 2022

A Terapia Comunitária pode ser definida como um espaço comunitário onde se procura partilhar experiências de vida e sabedorias. Objetivo de relatar a vivência, a importância e a eficácia da Terapia Comunitária no processo saúde-doença, pelas experiências vividas por alunas do curso de Medicina e de Psicologia da Universidade de Fortaleza, bolsistas do Programa de Educação Tutorial PET-SAÚDE Interprofissionalidade. Consiste em um relato de experiência, no qual a prática foi realizada na unidade de saúde de atenção primária à saúde Luís Franklin, em Messejana, em Fortaleza - Ceará, onde uma enfermeira da Estratégia Saúde da Família atua como terapeuta comunitária e uma Agente Comunitária de Saúde atua como co-terapeuta neste processo há 3 anos, e, através de um questionário estruturado verificou-se diversos relatos de experiência positivas dos participantes e a importância da terapia comunitária na vida desses pacientes. Os participantes do grupo analisado responderam que se sentem pertencentes a ele e descrevem o ambiente como local de acolhimento, valorização, tranquilidade, alegria, segurança e paz. Desse modo, conclui-se que a terapia comunitária consiste em uma prática integrativa e complementar eficaz e de fácil implementação na Atenção Básica, auxiliando no tratamento das diversas e complexas demandas presentes no contexto social brasileiro.
Tecnologia educacional: Orientação no autocuidado à pacientes idosos da alta da clínica médica para o domicílio
Authors: Santos José Daniel Rodrigues dos , Santos Valeria Regina Cavalcante dos , Mendonça Xaene Maria Fernandes Duarte , Ferreira Ilma Pastana , Albuquerque Jacirene Vasconcelos de , Siqueira Bianca Camila Neves , Coelho Vitória Fernanda Valadares , Gonçalves Brenda Laryssa Lima , Souza Cristina Frassinette Lima , Trindade Creusa Barbosa dos Santos
Published: August 2022

Objetivo: Construir uma cartilha educacional para orientação de autocuidado a pacientes idosos na alta da clínica médica para o domicílio Método: Trata de pesquisa-ação com abordagem quanti-qualitativa desenvolvida na FSCMP no setor de clínica médica.  A população do estudo foram 84 pacientes idosos com déficit no autocuidado, em processo de alta para o domicílio.  Para coleta de dados foi aplicado um instrumento semiestruturado, compondo dados sociodemográficos, econômicos, clínicos; internação, capacidade para o autocuidado, percepções sobre alta hospitalar e dificuldades no autocuidado. As entrevistas foram processadas segundo a análise de conteúdo do método de Bardin. As variáveis quantitativas foram distribuídas em frequências absolutas e relativas. Resultados: A amostra predominou idosos do sexo masculino (66,67%), faixa etária de 65 a 69 anos (42,85%), renda familiar de até 01 salário mínimo (53,57%), união estável (33,33%), grau de escolaridade do ensino fundamental (61,91%), pardos (78,54%) e aposentados (41,67%). A maioria possui tempo prolongado de internação sendo 33,34% superiores a 30 dias, 77,38% são polifarmácias, 46,43% apresentam dependência importante. O diagnóstico mais evidenciado foi doença do sistema digestivo (42,85%). Quanto a compreensão da hospitalização e alta hospitalar, os significados que os sujeitos atribuíram foram classificados em três categorias temáticas: A-A importância do apoio familiar/cuidadores; B–A manutenção financeira da família; C–Cuidados gerais referidos de autocuidado. Conclusão: Há necessidade de estudos e construção de mais tipos de produtos com tecnologias educativas que busque abranger a maior quantidade possível de orientações de autocuidado a pacientes idosos pós-alta hospitalar.
Um olhar renovado sobre o chamado divino a ‘cuidar e cultivar’: a questão da essência administrativa
Authors: Lovo Odirlei Arcangelo , Sanches Mário Antônio
Published: August 2022

O objetivo deste artigo é propor uma reflexão sobre o próprio ser humano, que é chamado a colaborar com Deus no cuidar e cultivar a criação. Apresenta-se a indagação: o que possibilita à pessoa ser um colaborador de Deus?  Trata-se de construção de narrativa, para qual os dois núcleos fundamentais de dados, textos e informações foram a Doutrina Social da Igreja e os documentos do Magistério que elucidam o projeto, a vivência e a dinâmica familiar. A narrativa apresenta que a pessoa, em esponsalidade com o Criador, se torna o ato administrativo de Deus no mundo, elucida-se, então, a ‘essência administrativa’, o dom primeiro, com o qual Deus dirige-se ao ser humano no chamado/mandado a cuidar e cultivar o jardim. Suscita-se a pessoa enquanto o ato administrativo de Deus no mundo, fomentando que é em família que os filhos/Ben de Deus devem cuidar da terra e se tronar a nação dos filhos/povo de Deus, nação sem limitações geográficas, alicerçada no amor e na confiança no Reino de Deus que se faz na contingência da criação sob a administração humana.
Tratamento da hipertensão arterial sistêmica: uma revisão de literatura integrativa sobre Hidroclorotiazida vs Clortalidona
Authors: Nascimento Yan Santiago , Nascimento Yago Santiago , Ferreira Letícia Maria Rendall , Cunha Neto Cícero Pereira , Santos Caio Roberto Alexandre Cavalcante dos , Menezes Arthur Souza , Santos Junior Ricardo Ferreira dos
Published: August 2022

Objetivo: Realizar uma revisão de literatura integrativa buscando analisar as principais diferenças no uso da Hidroclorotiazida vs Clortalidona, afim de entender se algum medicamento se sobrepõe sobre o outro. Metodologia: Foi realizada uma revisão de literatura integrativa, analisando-se artigos desde janeiro de 2010 até junho de 2022, nos idiomas português, inglês e espanhol nas seguintes bases de dados: MEDLINE via PubMed, Europe PMC, Lilacs e SciELO. Os descritores utilizados foram “Clortalidona”, “Hidroclorotiazida” e “Hipertensão”, de acordo com o DeCS, abrangendo ao final 31 artigos. Resultados: Diversos estudos apontam para o efeito mais intenso da clortalidona sobre a hidroclorotiazida, mas ressaltam que o primeiro possui maiores efeitos adversos, como hipocalemia e hiponatremia. Algumas pesquisas trazem que o uso da clortalidona sobre a hidroclorotiazida trouxe uma redução de risco cardiovascular de aproximadamente 18%, sendo um resultado bastante debatido na literatura por ser fruto de meta-análise indireta.  Conclusão: A literatura ainda diverge quanto ao uso da clortalidona sobre a hidroclorotiazida, necessitando de mais estudos conclusivos sobre o efeito direto comparativo entre as duas drogas. Os dados da literatura convergem que a clortalidona tem um efeito mais intenso que a hidroclorotiazida, trazendo, no entanto, mais efeitos adversos para o paciente, como hipercalemia, por exemplo.
UJI KUALITAS FISIK DAN ORGANOLEPTIK DAGING AYAM BROILER YANG DIBERI PAKAN TAMBAHAN DAUN TAHONGAI (Kleinhovia hospital L)
Authors: Wijaya Riyo , Manullang Julinda Romauli , Daru Taufan Purwokusumaning
Published: August 2022

Daging broiler sangat diminati oleh masyarakat luas pada saat ini, akan tetapi lemak pada broiler dapat menyebabkan kolesterol, tidak hanya itu lemak juga dapat menyebabkan sifat fisik dan organoleptik yang berbeda pada daging ayam. Tahongai merupakan tanaman khas kalimantan yang mengadung flavonoid, saponin dan tanin yang dapat berpengaruh pada kualitas fisik dan organoleptik daging ayam. Tujuan penelitian ini yaitu mengetahui pengaruh daun tahongai (Kleinhovia hospita L) terhadap kualitas fisik dan organoleptik ayam broiler. Penelitian ini telah dilaksanakan pada bulan November 2019. Pengujian kualitas fisik dan pengujian organoleptik dilaksanakan di Laboratorium Produksi dan Nutrisi Peternakan, Fakultas Pertanian Universitas Mulawarman, Samarinda. Penelitian dilakukan dengan cara membandingkan pelakuan tanpa tahongai (T1) dan pemberian Tahongai (T2) terhadap kualitas fisik dan organoleptik daging ayam broiler segar, data yang didapat diuji lebih lanjut dengan uji T. Hasil yang didapat ditahui jika penambahan tahongai mempengaruhi tekstur dan aroma daging ayam (P<0,05) dengan nilai masing-masing 3,58 (agak suka) dan 3,6 (agak suka) uji mutu hedonik berpengaruh tidak nyata (P>0,05) terhadap semua parameter.
CHARACTERISTICS OF BEEF CATTLE BREEDERS IN LAREH SAGO HALABAN DISTRICT, LIMA PULUH KOTA REGENCY
Authors: Sari Yesi Chwenta , Nanda Syafri
Published: August 2022

TKarakteristik peternak merupakan salah satu faktor yang mempengaruhi tingkat adopsi inovasi dalam pengembangan usaha peternakan. Penelitian ini bertujuan untuk mengetahui karakteristik peternak sapi pedaging berdasarkan faktor demografis yang ada di Kecamatan Lareh Sago Halaban, Kabupaten Lima Puluh Kota, Provinsi Sumatera Barat. Penelitian ini merupakan penelitian deskriptip kualitatif menggunakan metode survey yang dilaksanakan pada bulan September-Oktober 2020. Responden Penelitian sebanyak 63 orang yang di survey dari 3 Nagari atau Desa yaitu Nagari Balai Panjang, Nagari Batu Payuang, dan Nagari Labuah Gunung. Karakteristik peternak yang diteliti adalah usia peternak, jenis kelamin peternak, lama beternak, tingkat pendidikan, dan jumlah ternak yang dipelihara. Teknik analisis data yang digunakan dalam penelitian ini adalah analisis deskriptif kualitatif persentase. Hasil penelitian menunjukkan bahwa sebagaian besar peternak berusia 51-60 tahun (36.51%), memiliki jenis kelamin laki-laki (82.54%), beternak lebih dari 10 tahun (73.01%), memiliki tingkat pendidikan sampai SD (41.27%), dan jumlah ternak yang dipelihara 1 - 5 ekor (93.65%). Dapat disimpulkan bahwa peternak sapi pedaging di Kecamatan Lareh Sago Halaban berada pada usia produktif, dan jumlah ternak yang dipelihara masih dalam skala kecil.
Sex-related differences in connexin-43 expression in left ventricles of aging rats.
Authors: Tribulová N , Dupont E , Soukup T , Okruhlicová L , Severs N
Published: August 2022

Cardiac gap junctions have been implicated in maintaining intercellular electrical and metabolic couplings. The abnormalities in connexin-43 (Cx43) lead to conduction defects and contractile dysfunction. We have evaluated the expression and phoshorylation status of Cx43 in the left ventricular myocardium of male and female 16-month-old rats submitted to 14-week L-thyroxine (T4) treatment. Western blot analysis revealed the presence of fully or intermediately phosphorylated and unphosphorylated forms of Cx43. We have found no significant differences in Cx43 expression and phosphorylation between T4-treated and control untreated animals. However, expression of Cx43 was significantly higher in female compared to male rats. We conclude that T4 administration has no effect on Cx43 expression, but there are sex-dependent differences in Cx43 expression in the left ventricles between aging male and female rats.
Visual evoked potentials and event related potentials in congenitally deaf subjects.
Authors: Chlubnová J , Kremláček J , Kubová Z , Kuba M
Published: August 2022

The purpose was to test parameters of visual evoked potentials (VEPs) and of event-related potentials (ERPs) in deaf subjects to verify visual and cognitive CNS functions in a handicapped group of the population. Three types of visual stimuli (with dominating parvocellular or magnocellular system activation or with cognitive tasks) were used in the study. Six deaf persons (4 women, 2 men, mean age 17 years) and 6 persons with normal hearing (sex- and age-matched) were included in this pilot study. In all types of stimulation, latencies and amplitudes of main VEPs and ERPs components were evaluated. No significant latency differences were found. However, significantly reduced amplitudes were found in the occipital area for responses to motion and cognitive stimuli which might be interpreted as a part of functional reorganization of the extrastriate and cognitive cortical areas of deaf subjects.
Relationship between maximal oxygen uptake and oxygenation level in inactive muscle at exhaustion in incremental exercise in humans.
Authors: Yano T , Horiuchi M , Yunoki T , Matsuura R , Ogata H
Published: August 2022

The aim of the present study was to determine whether the oxygenation level in an inactive muscle during an incremental exercise test, determined by near-infrared spectroscopy, influences the maximal oxygen uptake (Vo2max). The oxygenation level at the onset of incremental exercise was higher than that at rest and started to decrease at a high power output. A minimal level was observed at exhaustion during incremental exercise. Vo2 increased linearly after some delay, and the rate of increase in Vo2 was greater at a higher power output. Heart rate increased linearly after the time delay, and the rate of increase in heart rate did not change. There was a significant correlation between Vo2max and oxygenation level in inactive muscle at exhaustion (r = -0.89). We therefore concluded that the oxygenation level in inactive muscle at exhaustion during incremental exercise is associated with an individual difference in Vo2max.
Evaluasi Usability Aplikasi Rudaya Menggunakan Metode Usability Testing dan USE Questionnaire
Authors: Walhidayah Irfan , Pradnyana I Made Ardwi , Raditya Putra I Gusti Lanang Agung
Published: August 2022

Rudaya merupakan platform yang menjembatani pencari jasa seni dengan seniman tradisional diseluruh Indonesia. Saat ini aplikasi Rudaya sudah diunduh oleh 150 pengguna yang terdiri dari berbagai kalangan, serta seniman dari berbagai daerah. Meningkatnya jumlah user aplikasi Rudaya dengan pengguna yang bervariasi, menimbulkan permasalahan baru terkait usability. Berdasarkan permasalahan terkait usability, dilakukan pengukuran usability menggunakan metode usability testing dengan melakukan evaluasi secara langsung pada sampel pengguna 10 responden. Pengujian dilakukan melalui tiga tahapan, tes task scenario, mengisi kuesioner dan wawancara. Pengujian juga menggunakan USE Questionnaire (Usefulness, Ease of Use, Ease of Learning, Satisfaction). Tahap akhir dilakukan perbaikan dengan menggunakan teori 8 golden rules dan 10 prinsip user interface aplikasi mobile, sehingga dihasilkan rekomendasi perbaikan prototype tampilan aplikasi Rudaya yang baru. Hasil pengujian usability pada success rate seniman pada pengujian awal 71,6%, Success rate pencari jasa seni 64%, Sedangkan success rate seniman pengujian akhir 93,3% dan success rate pencari jasa seni 90%. Untuk hasil time of working seniman dan pencari jasa seni rata-rata mengalami peningkatan pengerjaan waktu yang lebih cepat. Selanjutnya hasil pengujian usability berdasarkan USE Questionnaire yang dilakukan di awal diperoleh hasil 57,5%, terjadi perubahan yang signifikan pada pengujian kedua pada aplikasi Rudaya diperoleh nilai usability berdasarkan USE Questionnaire sebesar 89,4%.
China: Assessing the Goodness of a Political System with Chinese Characteristics
Authors: Kelleher William
Published: August 2022

Applying the Hartman-Easton Synthesis this paper illustrates how the goodness of China’s political system can be assessed. Using David Easton’s theory of the political system as if it were an automotive engineer’s blueprint for the expected operation of a motor, the operational goodness of any political system can be assessed as an empirical, rather than moral, matter. Robert S. Hartman, the founder of the science of values (“value science”), has shown that goodness can be assessed in three dimensions. As those pertain to political science, they are, the systemic, or constitutional; the extrinsic, or operational; and the intrinsic, or public sentience. The conclusion addresses the challenge of rendering an over-all, or general, assessment of a political system’s goodness.
Novel miniaturized fluorescence loop-mediated isothermal amplification detection system for rapid on-site virus detection
Authors: Wu Yanqi , Bai Liping , Ye Chengfu , Yuhong Guan  , Kunming Yan  , Chen Hui , Jiang Zhihong
Published: August 2022

New pathogen outbreaks have progressed rapidly and are highly infectious in recent years, increasing the urgency of rapid and accurate detection of pathogenic microorganisms. Based on the point-of-care testing (POCT) requirements, in this study, a real-time fluorescent loop-mediated isothermal amplification (LAMP) detection system was developed and applied to pathogen detection. The system is compact and portable, with good uniformity and reproducibility, and it can detect pathogens rapidly and effectively. For norovirus detection, the linear range was 100–106 copies/μL. The system can achieve the theoretical sensitivity of LAMP detection, conclusions could be obtained within 35 min, and quantitative detection was possible. The test results of 45 clinical samples were consistent with quantitative PCR (qPCR) and clinical results, and the accuracy could reach 100%. This system has the characteristics of portability, speed, and POCT accuracy, and the cost is much lower than that of commercial qPCR. Therefore, it is suitable for remote areas or places with relatively poor conditions and environments requiring on-site conditions. It can also be widely used to detect various epidemics and unexpected diseases.
Radiation therapy affects YAP expression and intracellular localization by modulating lamin A/C levels in breast cancer
Authors: La Verde Giuseppe , Artiola Valeria , Pugliese Mariagabriella , La Commara Marco , Arrichiello Cecilia , Muto Paolo , Netti Paolo A. , Fusco Sabato , Panzetta Valeria
Published: August 2022

The microenvironment of breast cancer actively participates in tumorigenesis and cancer progression. The changes observed in the architecture of the extracellular matrix initiate an oncogene-mediated cell reprogramming, that leads to a massive triggering of YAP nuclear entry, and, therefore, to cancer cell proliferation, invasion and probably to increased radiation-resistance. However, it is not yet fully understood how radiotherapy regulates the expression and subcellular localization of YAP in breast cancer cells experiencing different microenvironmental stiffnesses. To elucidate the role of extracellular matrix stiffness and ionizing radiations on YAP regulation, we explored the behaviour of two different mammary cell lines, a normal epithelial cell line (MCF10A) and a highly aggressive and invasive adenocarcinoma cell line (MDA-MB-231) interacting with polyacrylamide substrates mimicking the mechanics of both normal and tumour tissues (∼1 and ∼13 kPa). We report that X-ray radiation affected in a significant way the levels of YAP expression, density, and localization in both cell lines. After 24 h, MCF10A and MDA-MB-231 increased the expression level of YAP in both nucleus and cytoplasm in a dose dependent manner and particularly on the stiffer substrates. After 72 h, MCF10A reduced mostly the YAP expression in the cytoplasm, whereas it remained high in the nucleus of cells on stiffer substrates. Tumour cells continued to exhibit higher levels of YAP expression, especially in the cytoplasmic compartment, as indicated by the reduction of nuclear/cytoplasmic ratio of total YAP. Then, we investigated the existence of a correlation between YAP localization and the expression of the nuclear envelope protein lamin A/C, considering its key role in modulating nuclear deformability and changes in YAP shuttling phenomena. As supposed, we found that the effects of radiation on YAP nucleus/cytoplasmic expression ratio, increasing in healthy cells and decreasing in tumour ones, were accompanied by lower and higher lamin A/C levels in MCF10A and MDA-MB-231 cells, respectively. These findings point to obtain a deeper knowledge of the role of the extracellular matrix and the effects of X-rays on YAP and lamin A/C expression that can be used in the design of doses and timing of radiation therapy.
A novel electrochemical immunosensor based on PdAgPt/MoS2 for the ultrasensitive detection of CA 242
Authors: Cao Linlin , Lu Sumei , Guo Chengjie , Chen Wenqiang , Gao Yinan , Ye Diwen , Guo Zejun , Ma Wanshan
Published: August 2022

Dynamic monitoring of tumor markers is an important way to the diagnosis of malignant tumor, evaluate the therapeutic effect of tumor and analyze the prognosis of cancer patients. As a tumor marker of digestive tract, CA242 is often used to Assess the therapeutic effect of colorectal cancer and pancreatic cancer. In this study, immunosensor technology was used to detect CA242. PdAgPt nanocomposites, which have great advantages in biocompatibility, electrical conductivity and catalytic properties, were prepared by hydrothermal synthesis method. The prepared PdAgPt nanocomposites were loaded onto the surface of molybdenum disulfide (MoS2) with large surface area, and the new nanocomposites were synthesized. Using PdAgPt/MoS2 as signal amplification platform, the label-free CA242 electrochemical immunosensor has a wide detection range that extends from 1*10−4 U/ml to 1*102 U/ml and a low detection limit (LOD, 3.43*10−5 U/ml) after optimization of experimental conditions. In addition, the CA242 immunosensor designed in this study also performed well in the evaluation of repeatability, selectivity and stability, and was successfully used for the detection of CA242 in human serum sample. Therefore, the label-free electrochemical immunosensor constructed in this study has a broad application prospect in the detection of clinical biomarkers.
A PARP1-related prognostic signature constructing and PARP-1 inhibitors screening for glioma
Authors: Li Hui , Wang Zhenhua , Hou Yuanyuan , Xi Jianxin , He Zhenqiang , Lu Han , Du Zhishan , Zhong Sheng , Yang Qunying
Published: August 2022

The current standard treatments of glioma include surgical resection, supplemented with radiotherapy and chemotherapy, but the prognosis is poor. PARP-1 (Poly ADP-ribose polymerase 1) is a hot spot for cancer-targeted therapy and was reported to be significantly elevated in glioma. In this study, we analyzed the role of PARP-1 in DNA damage repair, constructed a PARP1-related DNA-repair prognostic signature (DPS), and screened targeted drugs for glioma. RNA-seq data of 639 glioma samples were downloaded from the GEO (Gene Expression Omnibus) database and divided into PARP1_H and PARP1_L according to the front and rear thirds of the expression level of PARP-1. First, we systematically analyzed the influence of PARP-1 on DNA damage repair, prognosis, and chemoradiotherapy sensitization of glioma. All glioma patients and patients with radiotherapy or chemotherapy had a better prognosis in PARP1_L than in PARP1_H. Next, differentially expressed DNA-repair related genes (DEGs) were identified between PARP1_H and PARP1_L by LASSO (Least Absolute Shrinkage and Selection Operator) Cox analysis and applied for constructing DPS. Based on the four-gene DPS, we then developed a new nomogram to assess overall survival in glioma patients. Additionally, PARP-1 was proved an effective target for glioma therapy. So, a series of computer-aided techniques, including Discovery Studio 4.5, Schrodinger, and PyMol, were applied for the virtual screening of favorable PARP-1 inhibitors. In conclusion, this study investigated the effect of PARP-1 on glioma prognosis and the sensitization effect of radiotherapy and chemotherapy, established a novel nomogram to evaluate the overall survival of glioma patients, and further explored targeted therapy for glioma.
Ligand-bound integrin αvβ6 internalisation and trafficking
Authors: Meecham Amelia , Cutmore Lauren C. , Protopapa Pantelitsa , Rigby Lauren G. , Marshall John F.
Published: August 2022

The integrin αvβ6 is expressed at low levels in most normal healthy tissue but is very often upregulated in a disease context including cancer and fibrosis. Integrins use endocytosis and trafficking as a means of regulating their surface expression and thus their functions, however little is known of how this process is regulated in the context of αvβ6. As αvβ6 is a major target for the development of therapeutics in cancer and fibrosis, understanding these dynamics is critical in the development of αvβ6-targeted therapies. Following development of a flow cytometry-based assay to measure ligand (A20FMDV2 or LAP)-bound αvβ6 endocytosis, an siRNA screen was performed to identify which genes were responsible for internalising αvβ6. These data identified 15 genes (DNM2, CBLB, DNM3, CBL, EEA1, CLTC, ARFGAP3, CAV1, CYTH2, CAV3, CAV2, IQSEC1, AP2M1, TSG101) which significantly decreased endocytosis, predominantly within dynamin-dependent pathways. Inhibition of these dynamin-dependent pathways significantly reduced αvβ6-dependent migration (αvβ6-specific migration was 547 ± 128 under control conditions, reduced to 225 ± 73 with clathrin inhibition, and 280 ± 51 with caveolin inhibition). Colocalization studies of αvβ6 with endosome markers revealed that up to 6 h post-internalisation of ligand, αvβ6 remains in Rab11-positive endosomes in a perinuclear location, with no evidence of αvβ6 degradation up to 48 h post exposure to A20FMDV2. Additionally, 60% of ligand-bound αvβ6 was recycled back to the surface by 6 h. With studies ongoing using conjugated A20FMDV2 to therapeutically target αvβ6 in cancer and fibrosis, these data have important implications. Binding of A20FMDV2 seemingly removes much of the αvβ6 from the cell membrane, and upon its recycling, a large fraction appears to still be in the ligand-bound state. While these results are observed with A20FMDV2, these data will be of value in the design of αvβ6-specific therapeutics and potentially the types of therapeutic load.
It takes two to tango: Widening our understanding of the onset of schizophrenia from a neuro-angiogenic perspective
Authors: Casas Bárbara S. , Arancibia-Altamirano David , Acevedo-La Rosa Franco , Garrido-Jara Delia , Maksaev Vera , Pérez-Monje Dan , Palma Verónica
Published: August 2022

Schizophrenia is a chronic debilitating mental disorder characterized by perturbations in thinking, perception, and behavior, along with brain connectivity deficiencies, neurotransmitter dysfunctions, and loss of gray brain matter. To date, schizophrenia has no cure and pharmacological treatments are only partially efficacious, with about 30% of patients describing little to no improvement after treatment. As in most neurological disorders, the main descriptions of schizophrenia physiopathology have been focused on neural network deficiencies. However, to sustain proper neural activity in the brain, another, no less important network is operating: the vast, complex and fascinating vascular network. Increasing research has characterized schizophrenia as a systemic disease where vascular involvement is important. Several neuro-angiogenic pathway disturbances have been related to schizophrenia. Alterations, ranging from genetic polymorphisms, mRNA, and protein alterations to microRNA and abnormal metabolite processing, have been evaluated in plasma, post-mortem brain, animal models, and patient-derived induced pluripotent stem cell (hiPSC) models. During embryonic brain development, the coordinated formation of blood vessels parallels neuro/gliogenesis and results in the structuration of the neurovascular niche, which brings together physical and molecular signals from both systems conforming to the Blood-Brain barrier. In this review, we offer an upfront perspective on distinctive angiogenic and neurogenic signaling pathways that might be involved in the biological causality of schizophrenia. We analyze the role of pivotal angiogenic-related pathways such as Vascular Endothelial Growth Factor and HIF signaling related to hypoxia and oxidative stress events; classic developmental pathways such as the NOTCH pathway, metabolic pathways such as the mTOR/AKT cascade; emerging neuroinflammation, and neurodegenerative processes such as UPR, and also discuss non-canonic angiogenic/axonal guidance factor signaling. Considering that all of the mentioned above pathways converge at the Blood-Brain barrier, reported neurovascular alterations could have deleterious repercussions on overall brain functioning in schizophrenia.
Multidimensional competition of nematodes affects plastic traits in a beetle ecosystem
Authors: Renahan Tess , Sommer Ralf J.
Published: August 2022

Resource competition has driven the evolution of novel polyphenisms in numerous organisms, enhancing fitness in constantly changing environmental conditions. In natural communities, the myriad interactions among diverse species are difficult to disentangle, but the multidimensional microscopic environment of a decaying insect teeming with bacteria and fighting nematodes provides pliable systems to investigate. Necromenic nematodes of the family Diplogastridae live on beetles worldwide, innocuously waiting for their hosts’ deaths to feast on the blooming bacteria. Often, more than one worm species either affiliates with the insect or joins the microbial meal; thus, competition over limited food ensues, and phenotypic plasticity provides perks for species capable of employing polyphenisms. The recently established system of cockchafer Gymnogaster bupthalma and its occasional co-infestation of Pristionchus mayeri and Acrostichus spp. has revealed that these worms will simultaneously utilize two polyphenisms to thrive in a competitive environment. While both genera maintain plastic capacities in mouth form (strictly bacterial-feeding and omnivorous predation) and developmental pathway (direct and arrested development, dauer), P. mayeri employs both when faced with competition from Acrostichus. Here, we took advantage of the malleable system and added a third competitor, model nematode Pristionchus pacificus. Intriguingly, with a third competitor, P. mayeri is quicker to exit dauer and devour available food, while Acrostichus hides in dauer, waiting for the two Pristionchus species to leave the immediate environment before resuming development. Thus, experimental manipulation of short-lived ecosystems can be used to study the roles of polyphenisms in organismal interactions and their potential significance for evolution.
Design of highly active Ni catalysts supported on carbon nanofibers for the hydrolytic hydrogenation of cellobiose
Authors: Frecha Esther , Remón Javier , Torres Daniel , Suelves Isabel , Pinilla José Luis
Published: August 2022

The direct transformation of cellulose into sugar alcohols (one-pot conversion) over supported nickel catalysts represents an attractive chemical route for biomass valorization, allowing the use of subcritical water in the hydrolysis step. The effectiveness of this process is substantially conditioned by the hydrogenation ability of the catalyst, determined by design parameters such as the active phase loading and particle size. Herein, mechanistic insights into catalyst design to produce superior activity were outlined using the hydrolytic hydrogenation of cellobiose as a model reaction. Variations in the impregnation technique (precipitation in basic media, incipient wetness impregnation, and the use of colloidal-deposition approaches) endowed carbon-nanofiber-supported catalysts within a wide range of Ni crystal sizes (5.8–20.4 nm) and loadings (5–14 wt%). The link between the properties of these catalysts and their reactivity has been established using characterization techniques such as X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy, and inductively coupled plasma-optical emission spectroscopy (ICP-OES). A fair compromise was found between the Ni surface area (3.89 m2/g) and its resistance against oxidation for intermediate crystallite sizes (∼11.3 nm) loaded at 10.7 wt%, affording the hydrogenation of 81.2% cellobiose to sorbitol after 3 h reaction at 190°C and 4.0 MPa H2 (measured at room temperature). The facile oxidation of smaller Ni particle sizes impeded the use of highly dispersed catalysts to reduce the metal content requirements.
Human circulating bacteria and dysbiosis in non-infectious diseases
Authors: Goraya Mohsan Ullah , Li Rui , Mannan Abdul , Gu Liming , Deng Huixiong , Wang Gefei
Published: August 2022

Blood microorganisms were once thought to indicate infection. Blood in healthy people appears to be devoid of growing bacteria; nonetheless, intracellular dormant forms of bacteria have been reported previously. With breakthroughs in sequencing and bioinformatics, the presence of bacterial DNA in healthy human blood initiated the controversy of human blood microbiota (HBM). Recently, bacteria-specific DNA and culturable bacteria were found in healthy human blood. Researchers wanted to study the phenomena of a “healthy blood microbiota” by providing a thorough description of bacterially produced nucleic acids using many complementing molecular and traditional microbiological approaches. Because blood is a relatively limited and particular environment, culturability and plate count issues can be overcome using enhanced cultured procedures. However, more evidence is required to confirm that healthy human blood contains normal microbiota. Cavities, mouth and intestinal microbiota, trauma, surgery, and animal/insect bites can introduce bacteria into human blood. All these factors strengthen the concept of transient blood bacteria too. The presence of blood bacteria may be caused by temporary immunological clearance and absorption by dendritic or M cells. This review provides an extensive and comprehensive analysis that suggests that healthy blood bacteria may not be typical microbiota but transient circulatory microorganisms. In this study, we look at how contaminants (Escherichia, Shigella, Pseudomonads, etc.) from the skin, laboratory environments, and reagents can affect the interpretation of blood-derived microbial information and the relationship between the circulating bacteria and non-communicable diseases. Circulating transient bacteria may play a role in the pathogenesis of non-infectious diseases such as diabetes and CVD. Contamination-free hematological studies can aid in understanding the disease mechanisms, therapy, and biomarkers.
Synergetic and charge transfer properties of a metal oxide heterojunction: Photocatalytic activities
Authors: Abebe Buzuayehu
Published: August 2022

Improvement in the synthesis techniques and their optimum properties to be up-to-date is the global need for industrially scalable applications. The sol–gel solution combustion synthesis (SG-SCS) approach is an easy, time-/energy-efficient, and creates regularly ordered porous materials that have significance in the ion-/mass-transport phenomenon. Furthermore, the approach also yields a decent heterojunction once optimized via the HSAB theory. Forming a heterojunction also tunes the crucial properties of the materials, thus, boosting the photocatalytic ability through charge transfer or/and synergistic roles. From the stability investigation results, the calcination temperature of 500°C is determined to be ideal. The X-ray diffraction and high-resolution transmission electron microscopy (HRTEM) techniques confirmed the nanoscale size of the NPs and NCs. The porous nature of the materials is revealed from the scanning electron microscopy micrographs and BET analysis; consistent results are also noted from selected area electron diffraction and HRTEM. The detected stacking faults on the IFFT image of HRTEM also confirmed the porous properties of the NCs. The precise elemental composition and local heterojunction within Zn/Fe(III)/Mn(III) oxides were confirmed in the HRTEM, X-ray photoelectron spectroscopy, and energy-dispersive X-ray studies. The significant charge transfer capability of the NCs more than bare ZnO was evidenced from the electrochemical analysis. The NCs were also effective on acid orange 8 (AO8) and Congo red (CR) dye degradations.
A fast least-squares reverse time migration method using cycle-consistent generative adversarial network
Authors: Huang Yunbo , Huang Jianping , Ma Yangyang
Published: August 2022

With high imaging accuracy, high signal-to-noise ratio, and good amplitude balance, least-squares reverse time migration (LSRTM) is an imaging algorithm suitable for deep high-precision oil and gas exploration. However, the computational costs limit its large-scale industrial application. The difference between traditional reverse time migration (RTM) and LSRTM is whether to eliminate the effect of the Hessian operator or not while solving Hessian matrix explicitly or eliminating the effect of the Hessian matrix implicitly has a very high requirement on computation or storage capacity. We simulate the inverse Hessian by training a cycle-consistent generative adversarial network (cycleGAN) to construct a mapping relationship between the RTM results and the true reflectivity models. The trained network is directly applied to the RTM imaging results, which improves the imaging quality while significantly reducing the calculation time. We select three velocity models and two velocity models respectively to generate the training and validation data sets, where the validation data is not involved in the training process. The prediction results on the validation data sets show that the trained network significantly improves the imaging quality with almost no additional in computational effort. Finally, we apply the network trained with only synthetics to the field data. The predicted results confirm the effectiveness and good generalization of the proposed method.