bluuebunny/crossref_metadata_2025 huggingface dataset:
Wpływ procesu ozonowania na parametry tekstury, aktywność antyoksydacyjną i właściwości organo-leptyczne dżemów z owoców borówki wysokiej i jabłek
Authors: Józefczyk Radosław , Szajna Anna , Balawejder Maciej
Published: November 2024

Owoce borówki wysokiej i jabłka poddano działaniu mieszaniny powietrza z ozonem. Ozon generowany był poprzez wyładowania koronowe w stężeniu 1, 10 i 100 ppm przez 5 minut w przypadku jabłek, stanowiących wsad i 30 min w przypadku borówki amerykańskiej będącej głównym surowcem do wykonania dżemów. Wykonane dżemy zostały poddane ocenie organoleptycznej na podstawie wybranych cech sensorycznych jak: barwa, zapach, smak, konsystencja, smarowność, faktura. Ocenie została poddana również tekstura dżemu, przy użyciu maszyny wytrzymałościowej, za pomocą testu TPA, podającego następujące parametry: twardość, siła niszcząca, praca ściskania, adhezyjność, sprężystość, gumistość. Wykonano również analizy biochemiczne, wyznaczając aktywność antyoksydacyjną wobec DPPH oraz zawartość polifenoli z użyciem metody Folina-Ciocialteu. Porównanie dżemów przygotowanych z owoców borówki i jabłek poddanych procesowi ozonowania atmosferą ozonową o stężeniach 1 ppm w czasie 5 minut dla jabłek oraz 10 i 100 ppm dla owoców borówki w czasie 30 minut, wykazało, że: 1) uprzednie ozonowanie nie ma istotnego wpływu na cechy sensoryczne wykonanych z nich dżemów, z wyjątkiem użycia najwyższej dawki ozonu (100 ppm), 2) wpływa w sposób istotny, ale co do kierunku niejednoznaczny i zależny od mierzonego parametru na teksturę wyznaczoną z użyciem standardowego testu TPA (twardość, siła niszcząca, praca ściskania, adhezyjność, sprężystość, gumistość), ze wskazaniem na podniesienie twardości i wielkości pochodnych. 3) wpływa w sposób istotny na aktywność antyoksydacyjną dżemów borówkowych oraz zawartość polifenoli dżemów jabłkowych. 4) ponadto, oceniane parametry sensoryczne i tekstury oraz aktywność antyoksydacyjna i zawartość polifenoli w gotowych dżemach, w istotny sposób zależą od rodzaju użytego owocu.
Wpływ rolnictwa na środowisko w opinii właścicieli wybranych gospodarstw rolnych położonych na terenie powiatu raciborskiego
Authors: Łuczak Katarzyna , Kusza Grzegorz , Błaszczok Marian
Published: November 2024

Celem niniejszej pracy było poznanie opinii i poglądów rolników na wybrane tematy związane z ochroną środowiska w rolnictwie. Badania przeprowadzono w 2024 r. metodą wywiadu kierowanego, a głównym narzędziem badawczym była ankieta. Badaniami objęto rolników trzech gmin powiatu raciborskiego: Kuźnia Raciborska, Krzyżanowice i Nędza.
Respondenci nie wykazują pełnej świadomości zagrożeń występujących w lokalnym środowisku. Badania wskazują, że rolnicy w ostatnich latach zwiększyli ilość stosowanych środków ochrony roślin w tym pestycydów, fungicydów oraz herbicydów. Tendencja ta ma związek z uodpornieniem się chwastów na substancje czynne zwarte w tych środkach.
Kolorowe łabędzie, innowacje, technologia. Rola nowych czynników wytwórczych
Authors: Zysk Wojciech
Published: November 2024

Współczesny świat jest coraz mniej zrozumiały dla człowieka i dla biznesu. Mówi się, że żyjemy w wieku nieciągłości. Ciągłe perturbacje otoczenia skłaniają do stwierdzenia, że mierzymy się z chaotycznym modelem prowadzenia biznesu, w tym międzynarodowego. Doświadczamy zdarzeń nieoczekiwanych i nieprzewidywalnych, które mają jednak wpływ na gospodarki i życie społeczne. Występują napięcia pomiędzy wiedzą o zjawiskach i problemach a fachową wiedzą o metodach zapobiegania czy rozwiązywania trudności, jak i przewidywania zdarzeń. Generuje to niepewność modeli biznesowych w gospodarce światowej. „Mutujące łabędzie” sprawiają, że pokojowa dywidenda (zysk z ograniczenia zbrojeń) maleje, a procesy globalizacyjne cofają się. Wdrażane innowacje, zwłaszcza technologiczne, stają się wyzwaniem dla biznesu. Poszczególne kraje mierzą się z nierównościami w rozwoju społeczno-gospodarczym w zależności od struktury gospodarczej, możliwości technicznych i technologicznych, etapu rozwoju i poziomu nakładów na badania. Dotychczasowe źródła i czynniki trwałej przewagi konkurencyjnej, czyli zasoby naturalne (np. ziemia), zasoby kapitałowe, zasoby ludzkie (praca) i przedsiębiorczość – czyli czynniki wytwórcze są zastępowane przez innowacje i rozwiązania technologiczne. Niektóre z nich, na przykład sztuczna inteligencja (AI), mogą stać się zarówno przydatnym narzędziem w bardziej zrównoważonym rozwoju gospodarczym, jak i bronią.
Las Miyawaki jako sposób retardacji zmian spowodowanych nadmierną urbanizacją
Authors: Czerniakowski Zbigniew W.
Published: November 2024

Intensywnie rozbudowujące się miasta są miejscem, w którym często napotykamy tereny o silnie zdegradowanych glebach. Założenie w takich miejscach terenów zieleni sprawia zwykle sporo kłopotów. Alternatywnym rozwiązaniem może być oddanie ich we władanie tak zwanej czwartej przyrody. Jeszcze ciekawszym pomysłem, z ekologicznego punktu widzenia, może być las kieszonkowy, nasadzenie zaproponowane przez japońskiego botanika Akira Miyawakiego. Wprawdzie początkowe etapy zalesienia są dość kosztowne i pracochłonne, szybko jednak możemy osiągnąć teren o znacznej ekofunkcjonalności. Powierzchnia lasu kieszonkowego może mieć zaledwie kilka m2 i wyśmienicie daje się wkomponować w krajobraz miasta. Jest znakomitym przykładem na próbę retardacji niekorzystnych zmian powodowanych przez nadmierną urbanizację.
Optymalizacja i walidacja metody oznaczania polichlorowanych bifenyli w glebie
Authors: Hućko Agnieszka , Szpyrka Ewa
Published: November 2024

Polichlorowane bifenyle (PCB) są to syntetyczne związki aromatyczne produkowane w dużych ilościach na całym świecie od lat trzydziestych XX wieku. Ich synteza została zabroniona w latach 70 XX wieku. Związki te miały szerokie zastosowanie między innymi jako płyny dielektryczne w kondensatorach i transformatorach, smary czy plastyfikatory. Ze względu na właściwości tych związków, takie jak odporność na degradację chemiczną i długi okres półtrwania (od 3 do 40 lat), stanowią one trwałe zanieczyszczenia środowiska. Najwyższe ich ilości znajdują się w glebie, jednak można je oznaczyć również w powietrzu, osadach, wodzie, roślinach, a nawet organizmach żywych. Celem pracy była optymalizacja i walidacja metody oznaczania kongenerów PCB 10, 28, 138, 153 i 180 w glebie. W procesie optymalizacji metody, testowano trzy odczynniki do ekstrakcji oraz dwa rodzaje sorbentów do oczyszczania. Walidację przeprowadzono na dwóch poziomach wzbogacenia w celu określenia odzysku i precyzji metody. Zoptymalizowaną i zwalidowaną metodę zastosowano do analizy zawartości wybranych kongenerów PCB w próbkach rzeczywistych pobranych z terenu Podkarpacia. Na podstawie przeprowadzonych badań wykazano, że najlepszym odczynnikiem do ekstrakcji PCB z gleby jest heksan, zaś sorbentem do oczyszczania Florisil. Stężenie PCB 10, 28, 52, 138 i 153 w analizowanych próbkach rzeczywistych było poniżej granicy oznaczalności (LOQ<0,005 mg/kg). PCB 180 oznaczono na poziomie 0,007 mg/kg w glebie pobranej z terenu obok linii kolejowych w Głuchowie.
Research of Options for Constructing Information Management Systems Based on Network Models of Queuing Systems
Authors: Kurakin Sergey , Onufrey Andrey , Razumov Alexander
Published: November 2024

The use of information management systems (IMSs) for the management of technical facilities is currently one of the directions for further improvement and increase in the effectiveness of the use of technical facilities in solving their target tasks. The existing modern IMSs are a set of hardware and software tools designed for collecting, processing and storing information and management. In the presence of a large amount of information and contradictory factors affecting the quality of management, making informed and timely decisions in the management process is impossible without the use of IMSs. The IMSs currently being developed are, for the most part, specialized systems and are designed to solve specific tasks. In this regard, the development and design of IMSs should be carried out taking into account the relationship with the target indicators and features of the management facilities, the results of a comprehensive analysis of information about the IMS elements in the process of functioning, and structural and algorithmic parameters that affect performance indicators. The use of mathematical models for the study of options for the construction of an IMS is the basis for the design and development of devices and subsystems of an IMS. The IMS models currently being developed make it possible to conduct research for single-stage management processes with the presence of similar service facilities in the system. At the same time, modern technical facilities and control systems are complex complexes with cyclically repeating control processes of various types of means. As a rule, such complexes have a set of parallel operating devices (control channels) that provide control of different types of objects at various stages of information processing. In this case, the structure of the IMS must be represented as a multiphase multichannel technical system in which the process of simultaneous management of several objects of various types takes place. In this regard, the purpose of the article is to develop a mathematical model of an IMS with two phases of management and the presence of an arbitrary number of serviced different types of management facilities. The basis of the model is a multiphase CFR network model with a limited waiting time for an application in the service queue. The study on the model allows choosing an option for building an IMS, in particular, choosing the optimal number of control channels for various types of objects according to the criterion of optimality and restrictions on the cost and time of management. An algorithm for selecting an option for building an IMS has been developed, and an example of calculating the number of control channels for managing three types of objects is given.
Enhanced Machine Learning Framework for Autonomous Depression Detection Using Modwave Cepstral Fusion and Stochastic Embedding
Authors: Jacob Jithin , Kannan K.S.
Published: November 2024

Depression is a prevalent mental illness that requires autonomous detection systems due to its complexity. Existing machine learning techniques face challenges such as background noise sensitivity, slow adaptation speed, and imbalanced data. To address these limitations, this study proposes a novel ModWave Cepstral Fusion and Stochastic Embedding Framework for depression prediction. Then, the Gain Modulated Wavelet Technique removes background noise and normalises audio signals. Difficulties with generalisation, which results in a lack of interpretability, hinder extracting relevant characteristics from speech. To address these issues, an Auto Cepstral Fusion extracts relevant features from speech, capturing temporal and spectral characteristics caused by background voice. Feature selection becomes imperative when choosing relevant features for classification. Selecting irrelevant features can result in overfitting, the curse of dimensionality, and less robustness to noise. Hence, the Principal Stochastic Embedding technique handles high-dimensional data, minimising noise influence and dimensionality. Furthermore, the XGBoost classifier differentiates between depressed and non-depressed individuals. As a result, the proposed method uses the DAIC-WOZ dataset from USC for detecting depressions, achieving an accuracy of 97.02%, precision of 97.02%, recall of 97.02%, F1-score of 97.02%, RMSE of 2.00, and MAE of 0.9, making it a promising tool for autonomous depression detection.
Cytokine expression profile of native heart valves obtained from patients with infective endocarditis
Authors: Sinitskaya A. V. , Sinitsky M. Yu. , Kazachek Ya. V. , Evtushenko A. V. , Khutornaya M. V. , Asanov M. A. , Poddubnyak A. O. , Ponasenko A. V.
Published: November 2024

Infectious endocarditis is characterized by dysfunction of heart valves and contribute significantly to the cardiovascular morbidity and mortality worldwide, especially in low- and middle-income countries. Immune response is playing the important role in the pathophysiology of this disease. This work was aimed to study the local cytokine profile in native heart valves obtained from the patients with infectious endocarditis. Cytokine profiling were performed in biopsies of native heart valves explanted from 4 patients with infective endocarditis (experimental group) and 10 patients with rheumatic heart disease (comparison group) by dot blotting using the Proteome Profiler™ Human Cytokine Array Kit (ARY005B). The results of dot blotting were validated by the gene expression profiling using quantitative polymerase chain reaction. MIF, PAI-1, ICAM-1 and CXCL12 were found in the native heart valves explanted from both infective endocarditis and rheumatic heart disease patients. Upon a semi-quantitative assessment, the heart valves explanted from the infective endocarditis patients were characterized by 4-fold increased secretion of PAI-1 and twofold decreased secretion of ICAM-1 and CXCL12 compared to the heart valves ffected by rheumatic heart disease. MIF was expressed on similar levels in the both studied groups. IL-1ra, IL-6, IL-8, IL-16, CCL4, CCL5 and CXCL1 were detected only in heart valves affected by infectious endocarditis. At the gene expression level, MIF, IL6, IL8 genes were upregulated and PAI1, IL1RA, CXCL1 genes were downregulated in heart valves explanted from infectious endocarditis patients compared to the subjects affected by rheumatic heart disease. Native heart valves in cases of infectious endocarditis are characterized by nonspecific local inflammatory response associated with pathogenic bacteremia, along with active neovascularization. The data obtained can help to better understand fundamental pathogenetic mechanisms of infectious endocarditis.
Cytokine profiling of plasma in patients with viral hepatitis C
Authors: Arsentieva N. A. , Batsunov O. K. , Lyubimova N. E. , Basina V. V. , Esaulenko E. V. , Totolian Areg A.
Published: November 2024

Chronic hepatitis C (CHC) represents a significant public health concern. In the majority of cases, the infection progresses to a chronic form, which is characterised by the development of fibrosis and cirrhosis of the liver. A plethora of cytokines and chemokines are generated as a consequence of inflammatory processes within the liver. These can exert a dual effect, both protective and damaging, particularly in relation to the death of hepatocytes and the progression of liver fibrosis. Furthermore, a number of growth factors have been identified as playing a role in the pathogenesis of CHC. The objective of the study was a comprehensive evaluation of a wide range of cytokines, chemokines and growth factors in the blood plasma of patients with CHC at varying stages of liver fibrosis. The study cohort comprised 63 patients diagnosed with CHC, who were divided into three groups according to the stage of liver fibrosis. The control group comprised healthy individuals (n = 32). Concentrations of the following cytokines were determined in plasma: Interleukins and some cytokines (IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17-E/IL-25, IL-17F, IL-18, IL-27, IFNα, IFNγ, TNFα, TNFβ); chemokines (CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CCL7/MCP-3, CCL11/Eotaxin, CCL22/MDC, CXCL1/GROα, CXCL8/IL-8, CXCL9/MIG, CXCL10/IP-10, CX3CL1/Fractalkine) and growth factors (EGF, FGF-2, Flt-3L, G-CSF, M-CSF, PDGF-AA, PDGF-AB/BB, TGF-α, VEGF-A) by multiplex analysis based on xMAP technology. Nonparametric statistics methods were used for statistical analysis. As a result of the study, increased concentrations of cytokines IL-12 (p40), IL-15, IL-17E/IL-25, IL-27, IFNγ, TNFα, chemokines CXCL9/MIG and CXCL-10/IP-10 and growth factors FGF-2 and M-CSF were found at all stages of liver fibrosis. Elevated concentrations of cytokines IL-1α, IL-1β, IL-2, IL-6, IL-9, IL-10, IL-17F, IFNα, TNFβ, chemokines CCL2/MCP-1, CCL11/Eotaxin, CCL22/MDC and growth factors G-CSF, TGF-α, Flt-3L were found in severe liver fibrosis/cirrhosis. Correlation analysis revealed a relationship of high significance between the severity of liver fibrosis and the content of cytokines IL-6, IFNγ, TNFα, IL-7, chemokines CCL2/MCP-1, CCL11/Eotaxin, CXCL9/MIG, CXCL10/IP-10, CXCL1/GROα, growth factors TGF-α, PDGF-AA, PDGF-AB/BB. Thus, a certain profile of cytokines characteristic for CHC was revealed, cytokines, chemokines and growth factors significant for liver fibrosis in CHC were found.
Clinical value of serum interleukin-13 levels in vincristineinduced polyneuropathy in children with acute lymphoblastic leukemia
Authors: Koryakina O. V. , Kovtun O. P. , Bazarnyi V. V. , Kopenkin M. A.
Published: November 2024

Vincristine polyneuropathy is the leading neurotoxic effect when treating pediatric acute lymphoblastic leukemia (ALL). Recent studies have demonstrated involvement of immune system in pathogenesis of peripheral nervous system damage. Over recent years, there have been reports examining the relationship between interleukin-13 (IL-13) and development of toxic effects of chemotherapeutic drugs. Our objective was to assess clinical value of IL-13 level in children with vincristine polyneuropathy. The study included 27 children with ALL aged from 3 to 17 years, who received chemotherapy according to the conventional protocol. Plasma IL-13 levels were determined, and the values in two subgroups have been compared taking into account development of vincristine polyneuropathy. IL-13 content was assessed by multiparametric immunofluorescent analysis with magnetic microspheres (xMAP technology, Luminex 200, USA) and using ProcartaPlex Human Cytokine/Chemokine test system (Invitrogen, USA). Vincristine polyneuropathy in the study group was registered in the majority of children (n = 15) manifesting mainly at the induction stage of chemotherapy and presenting as predominance of sensory disorders. In a comparative analysis of IL-13 plasma levels in patients with vincristine polyneuropathy, we observed a statistically significant increase of its concentration, in contrast to patients without signs of peripheral nervous system damage (p = 0.042). The diagnostic sensitivity of this index was 75%, specificity – 100%, the integral index characterizing the accuracy of the test was 0.89. IL-13 changes were found to correlate with higher relative risk level, indicating its significant relationship to the development of vincristine polyneuropathy. The results of the study on the IL-13 content in blood plasma in children with vincristine polyneuropathy allowed us to consider it a predictive biological marker of peripheral nervous system damage.
Effect of 5-hydroxypyrimidine derivatives and reference drug Aerius on systemic anaphylaxis, concentration of immunoglobulin E and cytokines tested in Balb/c mice
Authors: Kovalenko L. P. , Zhurikov R. V. , Valdman E. A. , Nikitin S. V.
Published: November 2024

Imbalance of Th1/Th2 lymphocytes and cytokines is responsible for increasing rate of pathology, such as allergy, autoimmune processes and cancer. Pyrimidines have a wide spectrum of pharmacological activity, for example antiallergic properties. The aim of this study is to investigate effects of 5-hydroxypyrimidine derivatives on systemic anaphylaxis, concentrations of immunoglobulin E (IgE) and cytokines in comparison with Aerius in experiments on ovalbumin-induced systemic anaphylaxis model. Effects of 7-day course of intraperitoneal (i/p) administration of SNK-411, SNK-578 at the doses of 25 mg/kg and 50 mg/kg, Aerius (i/p, 1.3 mg/kg) were studied in 140 male Balb/c ovalbumin-sensitized mice. 120 mice were immunized by two injections (two-week interval) of ovalbumin (100 mg per mouse) mixed with aluminum hydroxide (5 mg per mouse). 20 mice were used as intact control. Administration of SNK-411, SNK-578, or Aerius began after second immunization and lasted for 7 days. Within one hour after last injection of the compounds, 70 mice received injection of ovalbumin (100 mg dissolved in 0.1 mL saline) in retroorbital sinus. 70 mice from control and experimental groups were euthanized by decapitation. IgE, Interleukin-2 (IL-2), IL-4, IL-5, IL-6, IL-10, IL-11, IL-17A, IL-17F, Interferon gamma (IFNγ), IL-13 concentrations were measured in blood sera. Administration of SNK-411, SNK-578 at the dose of 50 mg/kg decreased systemic anaphylaxis, lowered IgE concentration by 1.5-1.6 times compared to ovalbumin control group whereas Aerius did not reduce concentration of IgE. Administration of SNK-411, SNK-578 at 25 mg/kg, and 50 mg/kg, and Aerius decreased concentration of Th-2 cytokines (IL-4, IL-5, IL-13) compared to ovalbumin-treated control group. SNK-411, SNK-578 at the doses of 25 mg/kg and 50 mg/kg increased level of IL-2. SNK-578 at 50 mg/kg increased concentration of IFNγ up to intact control level and decreased concentration of IL-6. 7-day administration of 5-hydroxypyrimidine derivatives at the dose of 50 mg/kg decreased severity of systemic anaphylaxis. SNK-411, SNK-578, Aerius lowered levels of proallergic cytokines in serum of sensitized mice. In contrast to Aerius, the 5-hydroxypyrimidine derivatives decreased serum IgE concentrations.
Effect of the Rubicon protein on LC3-associated phagocytosis by monocytes in the patients with severe atopic bronchial asthma
Authors: Ibragimov B. R. , Skibo Yu. V. , Reshetnikova I. D. , Abramov S. N. , Daminova A. G. , Evtyugin V. G. , Abramova Z. I.
Published: November 2024

Atopic bronchial asthma is the most common and severe allergic disease among a wide range of similar diseases. The main pathogenesis of this disease is characterized by a disturbance of T lymphocyte homeostasis, which significantly worsens the general state of health. In atopic bronchial asthma, there is impaired process of T cell apoptosis. This entails dysregulation and maintenance of peripheral lymphocyte homeostasis. Normally, T cells must undergo apoptosis, and its products should be utilized by neighboring cells, or professional phagocytes: monocytes, macrophages, or dendritic cells. This process is altered in atopic bronchial asthma. The immune system disorders, such as autoimmunity, often result from dysregulation of lymphocyte apoptosis. This is especially true in cases of insufficient or missed clearance of apoptotic bodies. Recently, the research and medical communities pay much attention to efferocytosis, a form of phagocytosi which proceeds by removal of apoptotic cells by phagocytes by means of LC3-associated phagocytosis (LAP). This process initiates uptake of the particles due to interactions between the phagocyte plasma membrane receptors and apoptotic cell. Further on, a single-membrane phagosome is formed in the cell with the participation of certain autophagy proteins (Beclin-1, VPS34, UVRAG, ATG5, ATG12, ATG7, ATG4, ATG4, LC3). The phagosome is enriched with LC3 protein molecules and fused with lysosomes, in which the captured “cargo” is then lysed. As a part of our work, a detailed analysis of some key protein contents at the LAP pathway was carried out for peripheral blood monocytes of patients with severe bronchial asthma. It was found that the expression of Rubicon protein is increased, thus allowing to conclude that the LAP pathway is activated in monocytes of healthy donors, thus allowing phagocytosis of dying T cells. At the same time, the components characteristic of both autophagy and LC3-associated phagocytosis are activated in the monocytes of patients with severe atopic asthma. However, one should note that decreased expression of the Rubicon protein, a putative marker of LC3-associated phagocytosis, has been clearly confirmed.
Immune analysis of natural neurotropic autoantibodies in blood serum of patients with COVID-19 associated ischemic stroke
Authors: Rasulova Kh. A. , Rasulova M. A.
Published: November 2024

The aim of the present study was to perform a comparative quantitative analysis of natural neurotropic autoantibodies (Nabs) in blood serum of patients with COVID-19 associated ischemic strokes (IS) in dynamics of disease. A total of 150 consecutive patients with acute primary IS were included, being divided in two groups: 100 patients with IS on the background of COVID-19 pneumonia (main group No. 1) and 50 patients with IS without COVID-19 symptoms and positive viral test (comparison group No. 2). The stroke severity and consciousness were measured by NIHSS and Glasgow coma scale. In blood serum of patients (n = 110), we have studied the levels of IgG Nabs to NF-200, GFAP, S100β, MBP, receptors to dopamine, serotonin, choline, glutamate, GABA by means of ELISA technique. The blood samples for analyses were taken at the 5th, 14th and 28th days of disease. In group 1 (n = 80), the Nabs levels have been increased as follows: antibodies to NF-200 (132.9±4.1 CU), by 1.09 and 1.8 times; for GFAP (118.9±3.9 CU), by 1.4 and 2 times; S100β antibodies (129.5±10.2 CU), by 1.05 and 1.6 times; MBP antibodies (97.3±4.5 CU) were 1.14 and 1.6 times higher; antibodies to dopamine receptors (77.9±4.4 CU) in 1.2 and 1.6 times; to serotonin receptors (81.96±3.25 CU) in 1.2 and 1.4 times; choline receptor antibodies (61.42±3.6 CU) were increased 1.4- and 1.8-fold; to glutamate (85.28±4.25 CU) by 1.19 and 1.4 times; to GABA (82.4±5.2 CU) the increase was 1.5- and 1.8-fold, respectively, when compared with group 2 and controls. The time-dependent monitoring of Nabs level in patients with COVID-19 associated ischemic stroke showed the highest increase in Nabs to the S-100, NF-200 and MBP proteins at the day +28 following the brain stroke. In patients with COVID-19 associated IS, more enhanced production of serum autoantibodies to neuroproteins and neurotransmitter receptors was detected, which accompanied a worse course of IS and can be considered as a predictor of unfavorable outcome of disease.
Intravenous immunoglobulins and recombinant granulocyte-colony stimulating factor modulate expression of NK cytotoxic receptors
Authors: Davydova A. A. , Mikhailova V. A. , Kovaleva A. A. , Grebenkina P. V. , Tyshchuk E. V. , Zementova M. S. , Bespalova O. N. , Sokolov D. I. , Selkov S. A.
Published: November 2024

Natural killer cells (NK cells) are a population of innate immune lymphocytes capable of cytolysis of infected or transformed cells without prior sensitization. Natural killers are detected in various organs and tissues and may differ in phenotypic and functional characteristics depending on localization. For example, NK cells are the dominant population (up to 70%) of decidual lymphocytes in early pregnancy. NK cells are able to contact with trophoblast cells, exert cytotoxicity towards them, as well as regulate their invasion, contributing to spiral arteries remodeling and establishment of physiological blood flow between mother and fetus. The contribution of impaired NK cell functional activity to immune mechanisms of the reproductive disorders is widely discussed. Various drugs are used to treat infertility, including intravenous immunoglobulins (IVIG) and recombinant granulocyte colony stimulating factor (G-CSF). Increased rates of embryo implantation and higher frequency of successful gestation have been shown after treatment with these drugs. The effect of these drugs on NK cells phenotype and functional activity is assumed, thus requiring further studies on the effects of IVIG and G-CSF on the receptor profile of NK cells. The aim of this work was to evaluate expression of cytotoxic receptors on the NK-92 cells in presence of IVIG and recombinant G-CSF preparations. NK-92 cells were used as effectors, and trophoblast-derived JEG-3 line served as target population. The cells were co-cultured in presence of drugs, as well as without them. Expression of CD45, CD56, CD215, KIR2DL3, KIR2DS4, NKG2D, NKp44, NKp30 receptors by NK-92 cells was evaluated by flow cytometry. The number of NK-92 cells expressing NKG2D, NKp30, KIR2DL3 receptors and the expression intensity of NKG2D and NKp30 receptors were reduced in presence of IVIG preparations. The numbers of KIR2DL3+ and NKp44+ NK cells were reduced when supplied with G-CSF and trophoblast cells. The obtained results may be associated with both direct and indirect effects of the studied drugs on the NK cell phenotype.
Physiological quality of seeds of arabic coffee cultivars stored for a period of two years
Authors: Alixandre R. D. , Lima P. A. M. , Alixandre F. T. , Krohling C. A. , Fornazier M. J. , Guarçoni R. C. , Almeida T. F. R. , Dias R. S. , Anastácio A. S. , Alexandre R. S. , Ferreira A. , Lopes J. C.
Published: November 2024

Abstract The rapid loss of viability, coupled with the difficulties and limitations in conserving coffee seeds, are some of the challenges that nurseries are currently facing. Thus, the objective of this work was to analyze the physiological quality of stored Arabica coffee seeds from cultivars recommended for planting in Brazilian mountainous regions. Seeds from 10 Arabica coffee cultivars were used: Catucaí-785/15, Catucaí-2SL, Catucaí-24/137, Japan, Arara, Acauã, Catuaí IAC-81, Mundo Novo IAC-379/19, Catuaí IAC-62, and Caturra IAC-479. The seeds were collected in the Arabica coffee seed production field, in the municipality of Marechal Floriano-ES. They were subsequently processed and dried in the shade, at room temperature, until reaching a humidity of 35 ± 1%, analyzed and stored in a natural laboratory environment (25 ± 2 °C), for 24 months. The following were analyzed: seed water content, germination, germination speed index, electrical conductivity and potassium leaching. The experimental design used was completely randomized, with ten cultivars, two storage times, with four replications of 25 seeds. Storing arabica coffee seeds for 24 months results in a drop in the germination percentage. Stored seeds of the Catucai 24/137 and Arara cultivars showed germination percentages similar to those of newly harvested seeds. Seeds of the Catuai IAC-62 cultivar maintain vigor during storage. The stored seeds of the Caturra IAC-476 and Japi cultivars showed a reduction in physiological quality. Electrical conductivity and potassium leaching tests are efficient in identifying seeds in an advanced state of deterioration. Seeds of Arabica coffee cultivars stored for 24 months, under the conditions of the present study, produce abnormal seedlings.
Disclosure of socio-environmental scandals and corporate reputation
Authors: Oliveira William Augusto Sousa de , Botinha Reiner Alves
Published: November 2024

ABSTRACT This research aimed to investigate the use of neutralization of disclosure of socio-environmental scandals and its relationship with corporate reputation. No studies were found observing the strategies and motivations of socio-environmental disclosure from a theoretical lens like the literature on neutralization techniques. This study sought its relevance by investigating the disclosure and reputation maintenance strategies used by companies involved in national socio-environmental scandals, identifying various paths for information neutralization, guided by the origin of each case and business motivations, in addition to highlighting empirical findings that stimulate the advance in research on neutralization techniques in corporate reports. The aim is to assist students and researchers by providing new evidence for corporate processes that involve socio-environmental disclosure strategies and reputational management. As well as contribute to ensuring that stakeholders see voluntary socio-environmental information better and in a well-founded manner, seeking to qualify decision-making. Corporations involved in the main national socio-environmental scandals between 2014 and 2021 were analyzed. This is an exploratory and quali-quantitative study, with content analysis and statistical tests to investigate socio-environmental scandals. Neutralization techniques were identified in information from voluntary corporate reports by companies involved in socio-environmental scandals. In addition to finding a positive correlation between neutralized disclosure and the reputation of these companies in the year the scandal took place and in the year after, there was also an increased use of techniques after the event. We hope to contribute to the literature by proposing the insertion of neutralization techniques in the investigation of strategies involving voluntary disclosure and corporate reputation. With a practical approach, we seek to build a rather critical view regarding voluntary reports and business mechanisms that may disqualify the information contained in them.
As Missões: Modos Inventivos e Brincantes das Crianças Calungas
Authors: Rodrigues Breno Ayres Chaves , Jurdi Andréa Perosa Saigh , Borba Patrícia Leme de Oliveira
Published: November 2024

Resumo: Compreendendo a(s) infância(s) como uma construção social em um tempo-espaço e as crianças como sujeitos de direitos que participam ativamente dessa construção, ou seja, produzem cultura, acompanhamos, a partir da base teórica-metodológica etnográfica, o cotidiano de 4 crianças calungas . Buscou-se aprofundar conhecimentos sobre a realidade cotidiana da(s) infância(s) e do bairro que ali se engendram. Essas mesmas crianças permitiram o acesso e o convívio em suas casas, na circulação pelo bairro, no estar em espaços institucionais e privados, em conversas mais sérias sobre namoro, violência, cultura, lazer e direitos humanos. Dentre os resultados, destacaram-se os modos inventivos e brincantes de circular pelo bairro, nomeando essas aventuras brincantes de missões. Por meio delas, as crianças reivindicavam uma cidade menos adultocêntrica , transgredindo e inventando suas próprias regras de uso dos lugares por onde passavam. Aposta-se que pesquisas como essas possam subsidiar profissionais, serviços e políticas que atuem com crianças.
Access/Retention in Multifunctional Resource Rooms: a reality in Amazonas
Authors: Ferreira Dorimar Gomes , Santos João Otacílio Libardoni dos
Published: November 2024

ABSTRACT The purpose of this study was to learn about the access and retention of students who are the target audience of Special Education in Multifunctional Resource Rooms (MRRs), from the perspective of teachers, in the municipalities of the Rio Madeira Microregion in southern Amazonas. The Qualitative Descriptive approach was used to outline the research, and data were collected using a semi-structured interview script. The findings point to the role of families, as the school has difficulty sensitizing parents so that they see themselves as an important part of the inclusion process and can recognize the MRR as an important space for their children.
Adventure Body Practices in Undergraduate Physical Education Programs: a comparative study between UFSC and UFG
Authors: Inácio Humberto Luís de Deus , Bassani Jaison José
Published: November 2024

ABSTRACT The insertion of the Adventure Body Practices in the undergraduate Physical Education programs of the Federal University of Santa Catarina and Federal University of Goiás carried out during the curricular reformulation process in the last years was analyzed. The methodology used was Comparative Education. The Federal University of Goiás created an introductory course, while the Federal University of Santa Catarina created three – an introductory course, a licentiate-specific course, and an optional course. Both cases present similarities and differences in the understanding of the new guidelines and how this was reflected in their Curricular Pedagogical Projects, with a strong influence of the territories where the institutions are located. The article concludes with the identification of the advances in each higher education institution on this content from the perspective of Comparative Education.
Os Impactos da Covid-19 nos Estudantes Internacionais no Brasil e Portugal
Authors: Iorio Juliana Chatti , Silva Adelia Verônica , Morilas Luciana Romano , Carneiro Cynthia Soares
Published: November 2024

RESUMO Vários estudos têm enfatizado a importância de se pesquisar as consequências da pandemia na internacionalização do ensino superior, mas poucos abordaram as vulnerabilidades e estratégias dos estudantes envolvidos nesse tipo de mobilidade. Este artigo examina, de forma comparativa, os impactos da pandemia de covid-19 nos estudantes internacionais que estavam no Brasil e em Portugal, utilizando uma metodologia mista (questionários e entrevistas). Os resultados demonstram que a pandemia não só provocou estresse e ansiedade generalizados, mas também gerou formas adicionais de precariedade para os estudantes do ensino superior, especialmente os originários do sul global. A partir da identificação dessas vulnerabilidades e das respostas encontradas por esses estudantes, esperamos que as instituições de ensino superior, bem como os governos dos países de origem e destino, também consigam encontrar respostas que contribuam para uma maior equidade entre os diferentes grupos de estudantes internacionais, e entre estes e os seus pares nacionais.
Teachers’ working conditions: a rural school in the semi-arid region of Piauí
Authors: Meneses Vinicius de Paiva , Silva Alexandre Leite dos Santos , Lopes Suzana Gomes
Published: November 2024

ABSTRACT Teaching working conditions are the set of resources and circumstances necessary for teachers to carry out their work and have an impact on the teaching-learning process. The objective of the research presented in this article was to investigate the conditions of teaching work in a rural high school located in a semi-arid in Piauí, Brazil. The qualitative approach was conducted through interviews with seven teachers who work night shifts in an annex of school, which serves young people from different rural communities. It was found that the working conditions offered to teachers are unsatisfactory in terms of the functions they perform, the employment relationship, the workload, transport conditions, infrastructure and the environmental conditions of the school space. The results made visible working conditions and how they impacted teachers' practice, reflecting on the teaching-learning process.
“Children as Readers of the World”: diversity, class, gender, and participation
Authors: Gonçalves Carolina , Tomás Catarina , Almeida Aline
Published: November 2024

ABSTRACT The strategic utilization of postmodern illustrated literature has emerged as a crucial tool in facilitating a profound understanding of how children construct their comprehension of diverse perspectives within the realm of social dynamics. This article rigorously scrutinizes children's conceptualizations pertaining to a wide array of subjects, encompassing diversity, socioeconomic disparities, gender dynamics, and active participation. This examination was conducted within the comprehensive framework of the RED_SMOOTH Project (Horizon 2020, European Union), which involved a qualitative investigation involving a cohort of 22 second-grade primary education students situated in the city of Lisbon. This initiative entailed the deliberate implementation of a read-aloud intervention program, primarily designed to explore the intricate themes under consideration.
The Hybrid Education as a Methodology and its Mercantilist Face in Brazil
Authors: Lima Daniela da Costa Britto Pereira , Rodrigues Marina Campos Nori , Deus Karen Brina Borges de
Published: November 2024

ABSTRACT This text analyzes the country's recent hybrid education standardization policy to identify the concept established in the legal instruments and the possible implications of hybrid education in Brazilian education. With a qualitative approach, this study starts from the document analysis of the instruments issued by the Ministry of Education and the theoretical basis of Hybrid Education and technology-mediated education. In conclusion, it is evident that the documents forward a proposal of the hybrid as a “methodology” of the distance modality incorporated into face-to-face education without the specific regulation of follow-up and evaluation policies.
O Plágio a partir da Percepção dos Alunos da Educação Superior a Distância
Authors: Campanhão Ligia Maria Barrios , Medeiros Bruno Cesar Cassani , Santos Stefanny Batista dos , Tito Ana Yenny Molleapaza , Curcio Ana Paula , Belo Juliana Morais , Pereira Luís Eduardo Santos , Haas Celia Maria
Published: November 2024

RESUMO Este estudo teve como objetivo investigar o conhecimento do plágio por alunos de uma universidade de educação a distância. Para isso, aplicou-se um questionário aos alunos e analisaram-se os conteúdos de duas disciplinas e de documentos institucionais para identificar orientações sobre o plágio. Nas disciplinas o tema não é discutido e os documentos internos apresentaram orientações inconsistentes sobre o seu enfrentamento. Embora os alunos afirmem saber o que é plágio, a maioria o associa unicamente à cópia de conteúdo sem a devida referência ou citação. Segundo os alunos, os motivos principais que levam ao plágio são a falta de organização e motivação para os estudos e de competências e habilidades na escrita acadêmica.
Terra Incógnita: Mapeando a Atuação da Justiça Eleitoral. Primeiros Passos (1932-1933)
Authors: Ricci Paolo
Published: November 2024

Resumo Qual o papel da Justiça Eleitoral na condução das eleições? Esta pergunta orientou vários trabalhos sobre o período democrático atual. Entretanto, o conhecimento sobre a fase inicial da Justiça Eleitoral é quase que inexistente. Este artigo constitui uma tentativa preliminar de elucidar os “primeiros passos” da Justiça Eleitoral na condução da eleição de 1933. Partindo de um arcabouço analítico centrado em quatro aspectos – organização da eleição, imparcialidade, independência e rule-making –, e por meio de um levantamento de dados inéditos em jornais e fontes oficiais, o estudo revela que em 1932 a Justiça Eleitoral apresentava 1) um elevado comprometimento nos aspectos organizacionais, ainda que atuasse em condições de 2) relativa parcialidade, sobretudo no âmbito administrativo local. O artigo conclui propondo que o tema da democratização no Brasil deva ir além das dimensões clássicas discutidas pela literatura (sufrágio, voto secreto, competição política) e passe a incorporar o papel da Justiça Eleitoral, contribuindo para a construção de uma agenda de pesquisa em torno deste ramo especializado do Poder Judiciário.
Ativismo Multinível: Movimento LGBTI+ e a Circulação de Políticas Públicas
Authors: Pereira Matheus Mazzilli
Published: November 2024

Resumo Nas últimas décadas, observa-se uma intensa circulação de políticas para LGBTI+ no Brasil. Uma vez que tais políticas foram marcadas pela fraca capacidade de indução do Governo Federal, como explicar sua circulação entre níveis e unidades da federação? Para responder essa pergunta, o artigo analisa a trajetória de circulação dos centros de referência LGBTI+ e do “tripé da cidadania” em cinco unidades de observação a partir de dados de entrevistas e de análise documental. Os resultados indicam que o “ativismo multinível” foi fundamental para a circulação dessas políticas por meio: da construção de redes informais de interação que integram níveis; da formação de organizações multinível; da atuação em arenas que reúnem atores oriundos de múltiplos níveis; e do deslocamento e múltiplo pertencimento de atores entre níveis. O artigo defende, assim, a fertilidade de um “olhar multinível” para as relações entre movimentos sociais e políticas públicas.
Federalismo e Coalizão: A Representatividade dos Estados nos Gabinetes Ministeriais Brasileiros
Authors: Franz Paulo , Codato Adriano , Fernández Virginia
Published: November 2024

Resumo Estudos sobre coalizões estão focados exclusivamente no aspecto partidário das alianças políticas do presidente. O artigo analisa a presença proporcional dos estados nos gabinetes ministeriais formados nos governos de Fernando Henrique Cardoso, Lula da Silva e Dilma Rousseff. A partir do local de origem política de 265 ministros, desenvolvemos um índice para mensurar a taxa de coalescência federativa dos gabinetes. Esse índice estima a proporcionalidade da representação estadual das equipes ministeriais ano a ano nesses governos. Mais do que a sobrerrepresentação de alguns estados em relação ao peso efetivo de suas bancadas no Congresso Nacional, nossos resultados permitem comparar os valores da coalescência federativa com a coalescência partidária ao longo do tempo. O índice mostra que o presidente da República procura privilegiar certas facções estaduais dentro dos partidos da base aliada, expressando assim uma nova dimensão do presidencialismo de coalizão.
Práticas corporais na congada: permanências e transformações nas tradições da festa da Vila João Vaz, Goiânia - GO
Authors: Carvalho Cleber de Sousa
Published: November 2024

RESUMO O texto apresenta reflexões acerca das práticas corporais promovidas pelos congadeiros da Vila João Vaz, Goiânia – GO. Tem como propósito identificar aspectos simbólicos decorrentes dos processos de transformação e permanência que interferem nas práticas corporais, considerando-se as danças e os cantos realizados pelos congadeiros. Utilizando-se da pesquisa etnográfica foram realizadas observações, entrevistas e o uso de caderno de campo. Identificou-se que os congadeiros vivenciam suas tradições, por meio de experiências corporificadas que valorizam determinados aspectos criativos, assim como mantém vivas suas referências com a ancestralidade africana e afro-brasileira.
IMPACTO SOCIAL DE LAS BARRERAS EN EL PROCESO DE CERTIFICACIÓN DE DISCAPACIDAD EN COLOMBIA
Authors: González Ballesteros Lina María , Castellanos Roncancio Camila Andrea , Carvajal Guzmán Jeimmi Paola
Published: November 2024

Mediante un análisis cualitativo la investigación busca responder a la pregunta: ¿Cuáles son las principales barreras que enfrentan las personas con discapacidad en Colombia para acceder y usar el certificado de discapacidad y los beneficios asociados? Para ello, se realizó un estudio cualitativo que incluyó entrevistas semiestructuradas y grupos focales con cinco actores relevantes en el proceso de certificación: personas con discapacidad y sus familias, representantes de organizaciones de la sociedad civil, tomadores de decisiones, funcionarios de instituciones prestadoras de servicios. . . de salud (IPS) y profesionales de la salud. Dos son los principales aportes de esta investigación. El primero, se refiere a la ausencia de conocimiento en discapacidad por parte del médico personal que incide en la activación del proceso de certificación; el segundo, la conceptualización de las barreras tecnológicas y logísticas, especialmente en áreas rurales con escasa conectividad a internet. Las conclusiones sugieren que para superar estas barreras es fundamental adoptar un enfoque integral que considere las múltiples dimensiones de la discapacidad, mejorar la capacitación de los profesionales de la salud y simplificar los procesos administrativos. Es necesario promover campañas de sensibilización y educación para desmitificar la certificación y reducir el estigma asociado. Además, se destaca la importancia de mejorar la infraestructura tecnológica y garantizar que las políticas de certificación sean inclusivas y adaptadas a las realidades culturales y socioeconómicas de todas las comunidades, especialmente en zonas rurales y entre comunidades indígenas y afrodescendientes.