bluuebunny/crossref_metadata_2025 huggingface dataset:
Rancang Bangun Sistem Informasi Akuntansi Peminjaman Dana Koperasi Berbasis Web
Authors: Nursarifah Desi , Widanengsih Euis
Published: August 2023

Dalam era modernisasi dan globalisasi yang semakin pesat, teknologi komputer telah menjadi kebutuhan pokok perusahaan sebagai pengolah data dan informasi. Dalam menghadapi tantangan tersebut, kita dituntut untuk menggunakan teknologi secara efektif dan efisien guna menyelesaikan permasalahan yang ada. Salah satu contohnya adalah pemanfaatan teknologi komputer dalam meningkatkan kinerja Koperasi Unit Desa Darawolong Mandiri Karawang. Koperasi tersebut merupakan lembaga keuangan yang memberikan layanan peminjaman dana kepada warga Desa Darawolong Karawang. Saat ini, proses pengolahan data dan pencatatan transaksi di koperasi masih dilakukan secara konvensional menggunakan buku besar. Hal ini menyebabkan kurang efektifnya pendataan dan penginputan terhadap proses peminjaman dana. Untuk mengatasi permasalahan tersebut dan meningkatkan pelayanan kepada nasabah, diperlukan suatu sistem informasi berbasis web yang dapat menangani dan mengolah data dengan baik. Dalam penelitian ini, dirancang sistem informasi berbasis web menggunakan Bahasa pemrograman Laravel dan MySQL sebagai basis datanya. Sistem ini diharapkan dapat meningkatkan efektivitas dan efisiensi dalam pengelolaan data peminjaman dana, serta memberikan layanan yang lebih baik kepada nasabah. Dengan adanya implementasi sistem informasi berbasis web, Koperasi Unit Desa Darawolong Mandiri Karawang akan dapat memanfaatkan kecanggihan teknologi untuk mengoptimalkan proses peminjaman dana dan memberikan informasi yang akurat dan cepat kepada nasabah.
Responsi Pembelajaran Pada Mata Kuliah Aljabar Linear Terhadap Hasil Belajar Mahasiswa Fakultas Teknologi Universitas Battuta Berbasis WEKA
Authors: Nuary Singarimbun Roy , Hayana Hasibuan Eka
Published: August 2023

Mata kuliah Aljabar Linear merupakan salah satu mata kuliah fakultas teknologi yang harus dikuasai pada setiap program studi pada fakultas teknologi. Aljabar Linear ini termasuk ilmu matematika, sehingga dosen pengampuh harus menguasainya, kinerja mahasiswa merupakan hasil belajar dalam memahami aljabar linear, beberapa mata kuliah yang berkaitan dengan aljabar linear tidak baik hasil studinya, hal ini karena tidak dimaksimalkan dengan baik pada mata kuliah aljabar linear, bisa jadi karena studi mahasiswa terbatas dan belum menyempurnakan konsep yang terlibat. Program respon/tutorial dianggap sebagai salah satu solusi yang dapat diadopsi untuk memaksimalkan kinerja dan hasil studi mahasiswa. Memahami program jawaban/tutorial memungkinkan mahasiswa untuk mengeksplorasi konsep dan masalah yang memiliki hubungan lebih dalam. Tujuan dari penelitian ini adalah untuk menguji pengaruh program jawaban/tutorial terhadap pembelajaran pada mata kuliah aljabar linear. Performansi mahasiswa dalam pembelajaran aljabar linear sangat mempengaruhi hasil studi dan kemampuan membuat konsep, logika seperti halnya nanti di mata kuliah algoritma dan pemograman. Hasil penelitian ini menunjukkan bahwa penerapan program solusi/tutorial memiliki dampak yang signifikan pada hasil belajar mahasiswa fakultas teknologi di universitas battuta dengan nilai rata-rata pada nilai A = 3.325, B+ = 6.9, B = 8.135, C+ = 8.87 dan nilai rata-rata C = 4.185.
The Utilization Of The Simple Multi Attribute Rating Exploiting Ranks Can Enhance The Performance Of The Aco Algorithm
Authors: Nanda Ginting Subhan Hafiz , Mughnyanti Mayang
Published: August 2023

In a comparative study, the performance of the ACO algorithm and a modified genetic algorithm (MGA) were evaluated for solving the multiple salesman traveling problem (MTSP) using various datasets from TSPLIB. The results revealed that although the proposed algorithm did not achieve the best solution, it exhibited improved time efficiency as the dataset size increased. The objective of this study is to improve the performance of the ACO algorithm by integrating the SMARTER algorithm, which aims to find the optimal route and minimize travel time. The combination of these algorithms offers alternative path solutions that can be effectively applied to solve TSP case examples and advance the development of new algorithms that excel in identifying the closest path. The study utilized TSPLIB datasets ranging in size from 76 to 1002 cities, sourced from the Felts and Nelson Krolak repositories. Within this study, the ACO algorithm was employed to optimize the overall distance in the TSP dataset, while the SMARTER algorithm generated suggestions for the optimal routes based on the total trip distance calculated by ACO. Experimental results demonstrated that the ACO algorithm, combined with SMARTER, achieved an average time improvement of 74.09% compared to the MGA algorithm, representing the most optimal performance.
Pengaruh Kompensasi, Team Work Melalui Kepuasan Kerja Terhadap Produktivitas Kerja SDM Dalam Mendukung Pencapaian Target Perusahaan Pada Media Infobrand.Id
Authors: Ningsih Susilowati
Published: August 2023

Penelitian ini bertujuan untuk mengetahui apakah terdapat pengaruh kompensasi, kerjasama tim melalui kepuasan kerja karyawan terhadap produktifitas kerja mereka dalam mendukung pencapaian target di Media Infobrand.id. Sampel dalam penelitian ini sebanyak 22 karyawan. Hasil dari penelitian ini menunjukkan bahwa kompensasi, kerjasama tim dan kepuasan kerja berpengaruh terhadap kinerja karyawan dalam mendukung target yang dicanangkan perusahaan. Kompensasi berpengaruh positif dan signifikan terhadap kinerja karyawan. Kerjasama tim berpengaruh positif dan signifikan terhadap kinerja karyawan. Kepuasan kerja berpengaruh positif dan signifikan terhadap kinerja karyawan. Oleh karena itu, manajemen perusahaan harus memperhatikan dan meningkatkan kepuasan kerja karyawan. Dengan meningkatnya kepuasan kerja karyawan maka kerjasama tim akan terbangun karena karyawan merasa puas dengan pekerjaannya sehingga mereka pun menjadi lebih termotivasi untuk mengerjakan tugas-tugas mereka dengan baik.
Implementasi Algoritma Apriori Pada Pola Pemilihan Menu Di Murai Kupi Menggunakan Rapid Miner
Authors: Rambe Aripin , Syahputra Dinur , Harahap Baginda
Published: August 2023

Kafe Murai Kupi adalah perusahaan yang bergerak di bidang kuliner. Kafe Murai Kupi sendiri berkeinginan untuk membuat strategi bisnis yang baru. Aktivitas bisnis yang sedang berlangsung dinilai belum maksimal untuk meningkatkan pendapatan penjualan karena perusahaan belum dapat melakukan penjualan menu secara merata, menu ada yang laku terjual dan ada yang tidak habis terjual. Penjualan menu yang tidak laku terjual akan mengalami penumpukan stok bahan makanan di kitchen stok, hal ini mengakibatkan kapasitas penyimpanan kitchen stok berkurang dan biaya pemiliharaan lebih tinggi. Strategi pemasaran saat ini perusahaan harus mampu mempertahankan atau bahkan meningkatkan daya saing dengan perusahaan lain, salah satunya dengan menerapkan Analisis Data Mining menggunakan algoritma Apriori untuk menghasilkan konsep yang digunakan oleh perusahaan adalah product bundling pada tujuan akhirnya, konsep ini memungkinkan perusahaan untuk dapat menggabungkan antara dua menu atau lebih dalam satu paket menu kepada pelanggan dengan tujuan menu yang tidak laku akan ikut terjual. Hasil yang diharapkan mampu meningkatkan proses bisnis perusahaan sehingga menciptakan peningkatan terhadap kepuasan pelanggan, dan peningkatan daya saing dengan perusahaan lain, perusahaan dapat meningkatkan pendapatan perusahaan. Sistem mengharapkan perusahaan untuk melakukan ini Anda dapat secara efektif dan efisien membuat menu bundel dan meningkatkan penjualan di perusahaan.
Relationship Between Prenatal Exercises With The Incidence Of Morning Sickness In Pregnant Women At The Juliana Dalimunthe Clinic 2023
Authors: Baginda Baginda
Published: August 2023

The first trimester of pregnancy is the initial period for pregnant women to adapt to their pregnancy. Morning sickness in the morning which is usually called morning sickness often occurs in pregnant women in the first trimester. Nearly 50% of pregnant women experience nausea and vomiting from the start of pregnancy (Suryani, 2021). The purpose of this study was to identify the relationship between prenatal exercise and the incidence of morning sickness at the Juliana Dalimunthe Clinic in 2023. This type of research is a quantitative study using a cross sectional study approach. The population in this study were all pregnant women who visited the Juliana Dalimunthe Clinic from April to June 2023. The samples were obtained using the accidental sampling technique. The statistical test used was the chi-square test with a 95% confidence level (α = 0.05). The results showed that the majority of respondents took part in prenatal exercise as many as 27 respondents (84%), the majority of respondents did not experience morning sickness as many as 30 respondents (94%), there was a relationship between prenatal exercise and sickness with a p value of 0.003 <0.05. It is hoped that the Juliana Dalimunthe Clinic will always provide information to all pregnant women to take part in prenatal exercises. Prenatal exercise can help reduce mother's discomfort during pregnancy, especially the first trimester of pregnancy.
STEPAN HAIDUCHOK’S CONTRIBUTION TO THE FORMATION AND DEVELOPMENT OF VOLLEYBALL IN LVIV (1920S)
Authors: SOVA Andrii
Published: August 2023

Based on various sources (documents and publications of the «Sokil-Batko» (The Falcon-father) society in Lviv; the epistolary heritage of S. Haiduchok; publications, information messages and advertisements in the Lviv journals «Sokilski visti» (The Sokil news), «Dilo» (Businesses); educational and methodological works «Vidbyvanka» (Volleyball); documents and photos from a private archive) S. Haiduchok’s contribution to the formation and development of volleyball in the Ukrainian community of Lviv is researched. With the involvement of previously little-known documents, materials and photos, his biography in the 1920s was reconstructed. The lower limit was determined – S. Haiduchok’s interest in volleyball, the upper limit – the publication of the work «Vidbyvanka». Attention was drawn to the fact that with the active participation of S. Haiduchok as a physical education teacher at the branch of the Academic Gymnasium in Lviv, volleyball was popularized in the activities of the «Sagaydachny» sports club, the Plast clubs and branches. It is emphasized on his important work as an organizer and judge of volleyball competitions, which were held in the sports hall at Ruska, 20 and Sq. «Sokil-Batko» in Lviv. The history of the writing of the educational and methodological work «Vidbyvanka», which became the fi rst Ukrainian textbook on this sport in Galicia, is considered. It was established that Ivan Boberskyi, Ilko Kalichak, Daria Navrotska, Ivan Mryts and other fi gures of Ukrainian physical education helped him in his scientific research. Attention is drawn to special sports terms and phrases proposed by S. Haiduchok for use in Ukrainian volleyball discourse. It is proven that he took an active part in the development and popularization of volleyball in various youth organizations of Lviv, which eventually led to the fact that in the 1930s , volleyball gained popularity and became one of the leading sports played by Ukrainians at that time.
Ключові слова
Stepan Haiduchok, Galicia, Lviv, vidbyvanka (volleyball), physical education, sport, competition.
As competências colaborativas desenvolvidas para o trabalho em equipe dentro do Projeto Pontes no enfrentamento à COVID-19
Authors: Beduschi Mariana , Xavier Júlia Bezerra , Lago Luana Pinho de Mesquita , Mestriner Soraya Fernandes , Mestriner Junior Wilson
Published: August 2023

O estudo teve por objetivo investigar a disponibilidade para a aprendizagem interprofissional e o desenvolvimento de competências colaborativas para o trabalho em equipe no Projeto Pontes no contexto da COVID-19. Trata-se de um estudo descritivo, exploratório, e transversal de abordagem mista qualitativa e quantitativa, desenvolvido por meio da aplicação do Questionário de Medida da Disponibilidade para Aprendizagem Interprofissional com questões para caracterização do perfil e de entrevistas semiestruturadas, online, com estudantes de graduação e egressos de diferentes áreas da saúde, que participaram da gestão do Projeto Pontes entre os anos de 2020 e 2021. As entrevistas foram gravadas, transcritas e analisadas por meio da técnica de análise de conteúdo temática. Participaram do estudo 11 graduandos (2 de Odontologia e 9 de Medicina) e 2 pós-graduandos de Terapia Ocupacional, em sua maioria mulheres de 23 anos em média. Observou-se que os estudantes se situam em uma zona de conforto com disponibilidade para a aprendizagem interprofissional, que os fatores “Trabalho em Equipe” e “Atenção à Saúde” são diretamente proporcionais e que não houve significância estatística quando comparadas as respostas do curso de medicina e das demais áreas. Os dados qualitativos foram organizados nos temas: apreendendo com o outro, dialogicidade, possibilidades da formação e compromissos e aprendizagens do cuidado centrado no usuário. O contexto de aprendizagem interprofissional com cuidado centrado no usuário contribuiu para o desenvolvimento de competências colaborativas favoreceu o fortalecimento de políticas públicas inclusivas que se mostraram ainda mais necessárias dentro do contexto da COVID-19.
High-resolution CBCT assessing a maxillary central incisor with root bifurcation: A case report
Authors: Carneiro Ana Luiza Esteves , Spin-Neto Rubens , Salgado Daniela Miranda Richarte de Andrade , Meneses Núbia Rafaelle Oliveira de , López Edna Alejandra Gallardo , Fonseca Alice Souza Villar Cassimiro , Costa Claudio
Published: August 2023

Cone beam computed tomography (CBCT) is an imaging modality widely used in endodontics, since it provides three-dimensional images without superposition of structures. The maxillary central incisor usually presents a single root and one root canal, but anatomical variations can happen. The aim of the present paper is to report a high-resolution CBCT evaluation of an upper central incisor with root bifurcation. This paper shows a descriptive and qualitative assessment of high-resolution CBCT images for endodontic purposes. A 39-year-old male patient was referred to a radiologic clinic to acquire CBCT images of the right upper central incisor, as the referring clinician noticed an anatomical difference between the two upper central incisors on a panoramic radiograph and suspected of dens invaginatus. CBCT images were acquired in high resolution (voxel size of 0.08 mm) and a restricted field-of-view (4x4 cm). CBCT volume displayed an anatomical variation of the central incisor, which presented root bifurcation and two root canals, with a normal clinical crown.  The present case report shows a rare diagnosis of a maxillary central incisor presenting bifurcated root with two root canals, and to the best of the authors’ knowledge, this is the first case report to present high-resolution CBCT images with disclosed acquisition protocols to diagnose a bifurcated central incisor. The documentation of unusual cases has great didact value and contributes to information propagation. In the present case, high-resolution CBCT allowed a detailed evaluation of both roots and root canals, facilitating the clinician’s diagnostic task.
Understanding youth in sport
Authors:   , Smits Froukje Cathelijne
Published: August 2023

In my dissertation, I challenged several modes of thought about youth sports and their practices. I purposely chose to explore practices and activities that vary greatly: resistance to competitive sport and the oft-invisible processes that are part of unorganised sports and of organised sports. Instead of drawing on commonly used post-positivist frameworks, I described an alternative way to study youth sport. In doing so, I gave participants a voice and contributed to the theorising of youth sport. A showed how variety in the use of theories, especially those that provide insight into the complex practices of youth sport such as the application of tools based of a Foucauldian approach to reality, can be used to develop policy and change practices that currently may result in youths dropping out, being exploited or abused or missing pleasure in participation. Because youth sport is considered to be an important contributor to the development of youths in society, research and theorising need to go beyond investigating outcomes and, instead, critically examine its complexities. The various studies in my dissertation revealed how a Foucauldian lens can be used to investigate youth sport practices and what this lens has to offer to scholars, administrators and policymakers to enhance their understanding and ability to respond to issues in youth sport.
DESCRIPTION OF NOISE IN THE COMPRESSOR AND GENERATOR AREA AT PT PERTAMINA EP ASSET 3 TAMBUN FIELD – BEKASI
Authors: Marfiana Pipit
Published: August 2023

PT Pertamina EP Asset 3 Tambun Field is a state-owned company engaged in Production Exploration, in the process this company has tools that must pay attention to the noise level, namely Compressors and Gensets. Compressors and generators are very influential on hearing loss in workers, therefore efforts to prevent hearing loss must be carried out. The objectives to be known are to know the noise conservation program, Standard Operating Procedures (SOP) in implementing noise conservation, and Implementation of Noise Conservation in Compressor and Genset Areas. This research method uses direct observation, interviews and literature studies. The results show that the Hearing Conservation Program has 7 (seven) components, namely: noise exposure survey, noise control, worker training, personal protective equipment, audiometric examination, recording and reporting, program assessment. The procedure used refers to the Individual Work Procedure (TKI) for Noise Measurement. The implementation of the Noise Measurement Program is carried out by the contractor once every 1 (one) year or every time there is a process change or a new tool. The Hearing Conservation Program must continue to make continuous improvements. It should be noted in monitoring workers in the Genset and Compressor area so that workers always use hearing protection equipment.
Parent's Stigma Towards Childhood Tuberculosis: A Multicenter Survey from Eastern Bandung Regency
Authors: Melati Azalia , Rohmatin Titin , Putri Karina Grashiva , Asri Tika , Apriani Desi , Rakhmawati Windy
Published: August 2023

Aims: Tuberculosis-related stigma negatively influences tuberculosis prevention programs such as delayed diagnoses, poor treatment adherence, and decreased quality of life of patients. This descriptive quantitative study aimed to describe the stigma towards tuberculosis among parents.
Method: A total of 30 samples taken through accidental sampling were parents who had children (0-14 years) with tuberculosis who had been treated at the public health centers. A questionnaire adapted from Van Rie TB Stigma Scale (VTSS) was used and analyzed using descriptive analysis.
Result: Most parents (76.6%) had lower levels of stigma, while the others scored higher levels. Lower stigma levels can be caused by a mature mindset, good knowledge of TB information, and indicates higher hopes from parents to increase their care level for children’s development, health-seeking behavior, and support for children’s TB treatment until it is completed. Accordingly, higher stigma levels from parents might be a result of several external factors that can increase the lack of motivation for health-seeking behavior in their children’s TB treatment.
Conclusion:  The role of health workers is very important in reducing stigma. Health education about prevention and treatment of childhood tuberculosis is still needed because it is a source of fear and high stigma against childhood tuberculosis in society.
The Implementation of Hands and Feet Exercise Towards Pain Management Among DM Type II Patients with Peripheral Neuropathy in Indonesia
Authors: Sartika Mila , Suryana Ilham , Azam Rohman , Jumaiyah Wati
Published: August 2023

Aims: The prevalence of diabetes mellitus (DM) worldwide increased significantly every year, including Indonesia. The most common complication among patients with Type II DM was DPN (Diabetic Peripheral Neuropathy) with the severe symptom being pan. However, only limited studies discussed related topics about intervention to reduce pain. Aim. This research objective was to measure the effectiveness of hand and foot exercises toward pain among patients with Type II DM.  
Method: A quasi-experiment of pre-post test design with a control and intervention group was applied in this study. To obtain respondents, this research used convenience sampling.  This study is located in the inpatient ward of two hospitals in Indonesia located in suburban areas.  Two questionnaires used in this study are Michigan Neuropathy Screening Instrument (MNSI) and Visual Analog Score (VAS). The Visual Analog Score (VAS). 
Results: A total of 38 respondents were divided into the same number of intervention and control groups with each of them 19 respondents. The average pain scale in the 1st to 8th week of measurement has decreased gradually in both group. Based on the results of the Friedman Test there was an effect of applying hand and foot exercises in reducing patient pain and diabetic peripheral neuropathy among the intervention group (p< 0.00) but no statistically significant decreased in the control group (p> 0.000). These results are in line with the reference journal in the assessment of pain response in both groups. These exercise increase blood vessel circulation and muscle strength.
Conclusion: This intervention can be implemented among patients with Type II DM as one of the pain management in clinical settings.  The standard operational procedures with structured training must be done to implement this intervention.
Influence of Educational Videos on Knowledge and Attitude on Reproduktive Health in Adolescent
Authors: Nopyanti Eti , Sri Futriani Elfira , Suliati Suliati , Murtiani Farida , Dinar Widiantari Aninda
Published: August 2023

Aims: Reproductive health education is essential to improve knowledge and attitude to grow up and live healthily. Audiovisual media as teaching method is considered as effective way to deliver message and material for health topic in teenager. It was reported that 10-14 years-old adolescent in Jakarta has reached 874,865 people in 2020. High populated of adolescent increased higher-risk sexual behavior. Sexual and reproductive education aims to increase knowledge about the anatomy and physiology of the organs and also improve attitude to avoid high-risk sex behavior.  
Objective: We aim to observe the effect of educational videos on the knowledge and attitudes of adolescents about reproductive health.
Method: Quasi-experimental research with pre and post-test without control group. Research sample was 100 students of 231 Junior School Jakarta using systematic random sampling. Intervention was educational videos about healthy sex behavior.
Result: We found significantly increased average knowledge (15,91 vs 23,24, p= 0,0001) and attitude (34,12 vs 43,26, p= 0,0001) score after the intervention.  
Conclusion: There was an significant diffrence of adolescents' knowledge and attitudes about reproductive health after educational video interventions. School and teacher play an active role in reproductive health counseling activities for adolescents with the right media.
Post-Populism: Transitions from Populist to Personalist Authoritarian Rule
Authors: Balta Evren , Baturo Alexander , Kenny Paul
Published: August 2023

Populism is a political strategy in which political leaders directly mobilize support through mass communication, typically posing as the opponents of an entrenched political elite. Although a growing body of research shows that populists draw on their mass support to weaken executive constraints, less attention has been given to what follows this populist turn. Specifically, under what circumstances are populists capable of leveraging their erstwhile mass support into authoritarian rule over the long-term? In contrast to other studies that put the durability of formal and informal democratic institutions to the fore, and unlike most current research on populism, drawing on detailed studies of Vladimir Putin in Russia and Recep Tayyip Erdoğan in Turkey, we explain the personalization of power under populist leadership by examining how such leaders manage other elites; that is, we place the degree, timing, and nature of elite cohort replacement at the center of our argument.
Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
Authors: Nguyen Melanie , Le Mignon Maxime , Schnellbächer Alisa , Wehsling Maria , Braun Julian , Baumgaertner Jens , Grabner Martina , Zimmer Aline
Published: August 2023

S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a multi-omics study was performed on industrially relevant CHO-K1 GS cells throughout a fed-batch process, including metabolomic and proteomic profiling combined with multivariate data and pathway analyses. Multi-layered data and enzymatical assays revealed an intracellular SSC/glutathione mixed disulfide formation and glutaredoxin-mediated reduction, releasing Cys and sulfur species. Increased Cys availability was directed towards glutathione and taurine synthesis, while other Cys catabolic pathways were likewise affected, indicating that cells strive to maintain Cys homeostasis and cellular functions.
The caecal microbiota promotes the acute inflammatory response and the loss of the intestinal barrier integrity during severe Eimeria tenella infection
Authors: Tomal Florian , Sadrin Guillaume , Gaboriaud Pauline , Guitton Edouard , Sedano Laura , Lallier Nathalie , Rossignol Christelle , Larcher Thibaut , Rouille Elodie , Ledevin Mireille , Guabiraba Rodrigo , Silvestre Anne , Lacroix-Lamandé Sonia , Schouler Catherine , Laurent Fabrice , Bussière Françoise I.
Published: August 2023

Introduction

Coccidiosis, a disease caused by intestinal apicomplexan parasites Eimeria, is a threat to poultry production. Eimeria tenella is one of the most pathogenic species, frequently causing a high prevalence of opportunistic infections.

Objective

The objective of this study is to investigate the role of the microbiota in the pathogenesis of severe Eimeria tenella infection.

Methods

We have previously shown that microbiota can promote parasite development. To study the effect of the microbiota on the pathogenesis of this infection, we used an experimental condition (inoculum of 10 000 oocysts E. tenella INRAE) in which the parasite load is similar between germ-free and conventional broilers at 7 days post-infection (pi). Thirteen conventional and 24 germ-free chickens were infected. Among this latter group, 12 remained germ-free and 12 received a microbiota from conventional healthy chickens at 4 days pi. Caeca and spleens were collected at 7 days pi.

Results

Our results demonstrated caecal lesions and epithelium damage in conventional chickens at 7 days pi but not in germ-free infected chickens. Administration of conventional microbiota to germ-free chickens partially restored these deleterious effects. At day 7 pi, both infected conventional and germ-free chickens exhibited increased gene expression of inflammatory mediators, including IL15, IFNγ, TNFα and the anti-inflammatory mediator SOCS1, whereas the inflammatory mediators CXCLi2, CCL20, IL18, CSF1, NOS2, PTGS2, IL1β, IL6, the receptor CCR2, and the anti-inflammatory mediators TGFβ1 and IL10 were upregulated only in infected conventional chickens. Notably, the IL18, PTGS2 gene expression was significantly higher in the infected conventional group. Overall, the inflammatory response enhanced by the microbiota might be in part responsible for higher lesion scores. Epithelial tight junction protein gene expression analysis revealed a significant upregulation of CLDN1 with the infection and microbiota, indicating a potential loss of the intestinal barrier integrity.

Conclusion

These observations imply that, during E. tenella infection, the caecal microbiota could trigger an acute inflammatory response, resulting in a loss of intestinal integrity. Increase in bacterial translocation can then lead to the likelihood of opportunistic infections. Hence, modulating the microbiota may offer a promising strategy for improving poultry gut health and limiting caecal coccidiosis.
Higher docosahexaenoic acid levels lower the protective impact of eicosapentaenoic acid on long-term major cardiovascular events
Authors: Le Viet T. , Knight Stacey , Watrous Jeramie D. , Najhawan Mahan , Dao Khoi , McCubrey Raymond O. , Bair Tami L. , Horne Benjamin D. , May Heidi T. , Muhlestein Joseph B. , Nelson John R. , Carlquist John F. , Knowlton Kirk U. , Jain Mohit , Anderson Jeffrey L.
Published: August 2023

Introduction

Long-chain omega-3 polyunsaturated fatty acids (OM3 PUFA) are commonly used for cardiovascular disease prevention. High-dose eicosapentaenoic acid (EPA) is reported to reduce major adverse cardiovascular events (MACE); however, a combined EPA and docosahexaenoic acid (DHA) supplementation has not been proven to do so. This study aimed to evaluate the potential interaction between EPA and DHA levels on long-term MACE.

Methods

We studied a cohort of 987 randomly selected subjects enrolled in the INSPIRE biobank registry who underwent coronary angiography. We used rapid throughput liquid chromatography-mass spectrometry to quantify the EPA and DHA plasma levels and examined their impact unadjusted, adjusted for one another, and fully adjusted for comorbidities, EPA + DHA, and the EPA/DHA ratio on long-term (10-year) MACE (all-cause death, myocardial infarction, stroke, heart failure hospitalization).

Results

The average subject age was 61.5 ± 12.2 years, 57% were male, 41% were obese, 42% had severe coronary artery disease (CAD), and 311 (31.5%) had a MACE. The 10-year MACE unadjusted hazard ratio (HR) for the highest (fourth) vs. lowest (first) quartile (Q) of EPA was HR = 0.48 (95% CI: 0.35, 0.67). The adjustment for DHA changed the HR to 0.30 (CI: 0.19, 0.49), and an additional adjustment for baseline differences changed the HR to 0.36 (CI: 0.22, 0.58). Conversely, unadjusted DHA did not significantly predict MACE, but adjustment for EPA resulted in a 1.81-fold higher risk of MACE (CI: 1.14, 2.90) for Q4 vs. Q1. However, after the adjustment for baseline differences, the risk of MACE was not significant for DHA (HR = 1.37; CI: 0.85, 2.20). An EPA/DHA ratio ≥1 resulted in a lower rate of 10-year MACE outcomes (27% vs. 37%, adjusted p-value = 0.013).

Conclusions

Higher levels of EPA, but not DHA, are associated with a lower risk of MACE. When combined with EPA, higher DHA blunts the benefit of EPA and is associated with a higher risk of MACE in the presence of low EPA. These findings can help explain the discrepant results of EPA-only and EPA/DHA mixed clinical supplementation trials.
Multimodality imaging in diagnosing lipomatous atrial septal hypertrophy with atrial septal defect: a case report
Authors: Yu Yi , Ding Ming , Chen Jin-Lan , Wang Ting , Chen Yu-Han , Yang Xiao-Min , Chen Su-Yun , Wang Yue-Peng , Li Yi-Gang
Published: August 2023

Background

Lipomatous atrial septal hypertrophy (LASH) with atrial septal defect (ASD) is a rare congenital anomaly. Although LASH is a histologically benign cardiac lesion characterized by excessive fat deposition in the interatrial septum that spares the fossa ovale, it has been associated with supraventricular arrhythmias or sick sinus syndrome. Application of multimodal imaging is crucial for accurate diagnosis, appropriate treatment of LASH with ASD, and follow-up.

Case summary

A 68-year-old female patient presented with recurrent chest tightness and palpitation. Multimodal imaging revealed the characterizations of LASH and ASD. Two-dimensional transesophageal echocardiography showed a “dumbbell”-shaped involvement of the cephalad and caudal regions with sparing of a single secundum ASD. The septum with a brightness feature is an uncommon condition characterized by the deposition of unencapsulated fat cells in the atrial septum. Real-time four-dimensional transesophageal echocardiography reflected the lipomatous hypertrophy of the atrial septum and an oval-shaped ASD. Cardiac computer tomography angiography later confirmed this finding. The patient achieved a good clinical response with an ASD percutaneous occlusion guided by intracardiac echocardiography (ICE).

Conclusion

This case demonstrates a LASH combined with ASD. Multimodality imaging can provide an accurate diagnosis and may guide the procedure for precise occlusion.
Geometric and kinematic analysis of faults bordering the Andaman sea continental shelves: a 3D seismic case study
Authors: Guo Meng , Luan Xiwu , Zhang Huixing , He Bingshou
Published: August 2023

To clarify the tectonic evolution of M15 block in the Andaman Sea, we perform a delicate study of fault geometry and dynamics using a 3D seismic data. The data reveal eight sequence interfaces from the Early Oligocene to the Quaternary, large scale and multi angle extensional strike-slip faults, and a series of normal faults. The two large scale faults F1 and F2 start in the Eocene and end in the Quaternary, controlling the regional structure. The NNE-SSW strike-slip F1 fault belongs to the South Sagaing fault and the NNE-SSW strike-slip F2 is the eastern Andaman fault, the strike-slip movement of which are controlled by the impact of the collision between the Indian plate and the Eurasian plate. Through the analysis of the fault development history by the method of the ancient drop and the growth index, we find that most of the large or secondary scale faults reach the maximum drop and growth index in the Miocene, indicating that the Miocene is a significant period of plate collision enhancing and faults generating. The regional stress field is dominated by E-W tension. The continental crust has expanded rapidly from the Oligocene to the Miocene which results in the rapid subsidence of the crust. This regional stress intensity becomes weak after the Miocene. The activities of the faults caused a large difference in terrain height between the west and the east in the study area, forming a pattern of the western depression and the eastern terrace. Many NNE-SSW, NE-SW or NEE-SWW trend strike-slip faults and minor faults develop in the Miocene. It echoes the event that the convergence and subduction of the Indian plate from SW to NE direction led to the right rotation and N-NNE strike-slip of the West Myanmar block in the Miocene, thus forming a regional large strike-slip fault. All of the faults affect the structure of the region.
The relationships between dung beetles and monkeys in the Neotropical region
Authors: Halffter Gonzalo , Favila Mario E.
Published: August 2023

The relationship between dung beetles and arboreal mammals has been scarcely studied, and many of the reports refer to observations without a standardized methodology. The accelerated loss of tropical forests urges us to understand this mutualistic association. Using our studies on arboreal dung beetles in the Palenque Archaeological Zone-National Park, Mexico, as a baseline, we analyzed the information on arboreal dung beetles in Neotropical forests in Mexico and around the world. Canthon euryscelis Bates, 1867, Canthon angustatus Harold, 1867, Canthon subhyalinus Harold, 1867, and Canthon femoralis (Chevrolat, 1834) are the main species collected in trees of Palenque, Onthophagus maya Zunino, 1981 and other non-Scarabaeinae species were occasionally collected from trees in Palenque. The small Canthon species are skilled fliers strongly relationship with monkeys in Palenque and other tropical regions of Mexico and Central America. In South America, arboreal dung beetles are more diverse and include these and other dung beetle species associated with monkeys. Several dung beetle species of the genus Onthophagus have been reported in association with African monkeys. In India, several studies report a wide variety of dung beetle species associated with monkeys. In Australia and New Guinea, only some species of Macropocopris are described as being associated with arboreal marsupials, but in Borneo, several dung beetle species have been observed associated with arboreal marsupials, mostly in managed forests. In Madagascar, Arachnoides gandi is the only beetle species reported in trees. We need to formulate a systematic and comparative methodology to understand better how arboreal beetles search for food, where the food is located, and how brood balls are made, how male-female pairs meet and nest, and how they contribute to arboreal dung recycling.
Myeloid-specific blockade of notch signaling alleviates dopaminergic neurodegeneration in Parkinson’s disease by dominantly regulating resident microglia activation through NF-κB signaling
Authors: Liang Shi-Qian , Li Peng-Hui , Hu Yi-Yang , Zhao Jun-Long , Shao Fang-Ze , Kuang Fang , Ren Kai-Xi , Wei Tiao-Xia , Fan Fan , Feng Lei , Han Hua , Qin Hong-Yan
Published: August 2023

Yolk sac–derived microglia and peripheral monocyte–derived macrophages play a key role during Parkinson’s disease (PD) progression. However, the regulatory mechanism of microglia/macrophage activation and function in PD pathogenesis remains unclear. Recombination signal–binding protein Jκ (RBP-J)–mediated Notch signaling regulates macrophage development and activation. In this study, with an 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) hydrochloride-induced acute murine PD model, we found that Notch signaling was activated in amoeboid microglia accompanied by a decrease in tyrosine hydroxylase (TH)–positive neurons. Furthermore, using myeloid-specific RBP-J knockout (RBP-JcKO) mice combined with a PD model, our results showed that myeloid-specific disruption of RBP-J alleviated dopaminergic neurodegeneration and improved locomotor activity. Fluorescence-activated cell sorting (FACS) analysis showed that the number of infiltrated inflammatory macrophages and activated major histocompatibility complex (MHC) II+ microglia decreased in RBP-JcKO mice compared with control mice. Moreover, to block monocyte recruitment by using chemokine (C-C motif) receptor 2 (CCR2) knockout mice, the effect of RBP-J deficiency on dopaminergic neurodegeneration was not affected, indicating that Notch signaling might regulate neuroinflammation independent of CCR2+ monocyte infiltration. Notably, when microglia were depleted with the PLX5622 formulated diet, we found that myeloid-specific RBP-J knockout resulted in more TH+ neurons and fewer activated microglia. Ex vitro experiments demonstrated that RBP-J deficiency in microglia might reduce inflammatory factor secretion, TH+ neuron apoptosis, and p65 nuclear translocation. Collectively, our study first revealed that RBP-J–mediated Notch signaling might participate in PD progression by mainly regulating microglia activation through nuclear factor kappa-B (NF-κB) signaling.
Global research trends and focus on the link between rheumatoid arthritis and neutrophil extracellular traps: a bibliometric analysis from 1985 to 2023
Authors: Chang Yonglong , Ou Qinling , Zhou Xuhui , Nie Kechao , Liu Jinhui , Zhang Sifang
Published: August 2023

Rheumatoid arthritis (RA) is an autoimmune disease that currently has an unknown cause and pathogenesis, and is associated with many complications and a high disability rate. The neutrophil extracellular trap network (NETs) is a newly discovered mechanism that allows neutrophils to capture and kill pathogens. Multiple studies in recent years have highlighted its relevance to the progression of rheumatoid arthritis. Despite the growing number of studies indicating the crucial role of NETs in RA, there has been no bibliometric review of research hotspots and trends in this area. In this study, we retrieved articles related to NETs in RA from the Web of Science Core Collection (WoSCC) database from 1985 to 2023 and used visualization tools such as Citespace, VOSviewer, Tableau Public, and Microsoft Office Excel 2021 to analyze the data. After screening, we included a total of 416 publications involving 2,334 researchers from 1,357 institutions in 167 countries/regions, with relevant articles published in 219 journals. The U.S., China, and Germany are the top 3 countries/regions with 124, 57, and 37 publications respectively. Mariana J. Kaplan is the most published author, and journals such as Frontiers in Immunology and International Journal of Molecular Sciences have had a significant impact on research in this field. The clinical application of PAD enzymes and their inhibitors, and the drug development of NETs as therapeutic targets for RA is a trend for future research. Our study provides a comprehensive bibliometric analysis and summary of NETs in RA publications, which will aid researchers in conducting further scientific research.
RNAi-mediated knockdown of two orphan G protein-coupled receptors reduces fecundity in the yellow fever mosquito Aedes aegypti
Authors: Keyes-Scott Nia I. , Swade Kyle R. , Allen Lena R. , Vogel Kevin J.
Published: August 2023

G protein-coupled receptors (GPCRs) control numerous physiological processes in insects, including reproduction. While many GPCRs have known ligands, orphan GPCRs do not have identified ligands in which they bind. Advances in genomic sequencing and phylogenetics provide the ability to compare orphan receptor protein sequences to sequences of characterized GPCRs, and thus gain a better understanding of the potential functions of orphan GPCRs. Our study sought to investigate the functions of two orphan GPCRs, AAEL003647 and AAEL019988, in the yellow fever mosquito, Aedes aegypti. From our phylogenetic investigation, we found that AAEL003647 is orthologous to the SIFamide-2/SMYamide receptor. We also found that AAEL019988 is orthologous to the Trapped in endoderm (Tre1) receptor of Drosophila melanogaster. Next, we conducted a tissue-specific expression analysis and found that both receptors had highest expression in the ovaries, suggesting they may be important for reproduction. We then used RNA interference (RNAi) to knock down both genes and found a significant reduction in the number of eggs laid per individual female mosquito, suggesting both receptors are important for Ae. aegypti reproduction.
The diagnostic value of morphological features of fat deposition of sacroiliac joint steatosis in axial spondyloarthritis
Authors: Zhao Jiaoshi , Lin Churong , Liu Dong , Liu Budian , Chen Qilong , Gu Jieruo
Published: August 2023

Background

Findings of fatty lesions in the context of other imaging manifestations, especially bone marrow edema and erosions can effectively assist in the diagnosis of axSpA. Chemical shift-encoded MRI is a sequence which allows for the quantification of fat signal and has been applied in the imaging evaluation of the SIJ in axSpA. The objective of this study was to investigate the diagnostic performance of morphological features of fatty lesions visualized by CSE-MRI in the imaging evaluation of SIJ in axSpA.

Methods

Fatty lesions with morphological features (subchondral, homogeneity and distinct border) were assessed and recorded as a binary variable in each quadrant of the SIJ. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated for different morphological features as well as the anatomical distribution in patients with nr-axSpA and r-axSpA. T1-weighted images and CSE-MRI fat fraction maps were directly compared in the recognition of different morphological features.

Results

Eighty-two patients [non-SpA (n = 21), nr-axSpA (n = 23), r-axSpA (n = 38)] with lower back pain (LBP) were enrolled. Presence of the three morphological features of fatty lesions had a specificity of 90.48% in axSpA. The sensitivities of being subchondral, homogeneity and distinct border were 52.17, 39.13 and 39.13% in nr-axSpA on T1-weighted images. For patients with r-axSpA, the sensitivities reached 86.84, 76.32 and 57.89%. No significant difference was found in the distribution of fatty lesions between T1-weighted images and CSE-MRI. However, CSE-MRI fat fraction maps could detect significantly more fatty lesions with homogeneity (p = 0.0412) and distinct border (p = 0.0159) than T1-weighted images in the sacroiliac joint, but not subchondral lesions (p = 0.6831).

Conclusion

The homogeneity and distinct border are more relevant for the diagnosis of axSpA. Moreover, CSE-MRI could detect more typical morphological features of fatty lesions than T1-weighted images in showing these two features. The presence of all three features was more likely to be indicative of axSpA.
Potential benefits of medium chain fatty acids in aging and neurodegenerative disease
Authors: Dunn Ella , Zhang Biqin , Sahota Virender K. , Augustin Hrvoje
Published: August 2023

Neurodegenerative diseases are a large class of neurological disorders characterized by progressive dysfunction and death of neurones. Examples include Alzheimer’s disease, Parkinson’s disease, frontotemporal dementia, and amyotrophic lateral sclerosis. Aging is the primary risk factor for neurodegeneration; individuals over 65 are more likely to suffer from a neurodegenerative disease, with prevalence increasing with age. As the population ages, the social and economic burden caused by these diseases will increase. Therefore, new therapies that address both aging and neurodegeneration are imperative. Ketogenic diets (KDs) are low carbohydrate, high-fat diets developed initially as an alternative treatment for epilepsy. The classic ketogenic diet provides energy via long-chain fatty acids (LCFAs); naturally occurring medium chain fatty acids (MCFAs), on the other hand, are the main components of the medium-chain triglyceride (MCT) ketogenic diet. MCT-based diets are more efficient at generating the ketone bodies that are used as a secondary energy source for neurones and astrocytes. However, ketone levels alone do not closely correlate with improved clinical symptoms. Recent findings suggest an alternative mode of action for the MCFAs, e.g., via improving mitochondrial biogenesis and glutamate receptor inhibition. MCFAs have been linked to the treatment of both aging and neurodegenerative disease via their effects on metabolism. Through action on multiple disease-related pathways, MCFAs are emerging as compounds with notable potential to promote healthy aging and ameliorate neurodegeneration. MCFAs have been shown to stimulate autophagy and restore mitochondrial function, which are found to be disrupted in aging and neurodegeneration. This review aims to provide insight into the metabolic benefits of MCFAs in neurodegenerative disease and healthy aging. We will discuss the use of MCFAs to combat dysregulation of autophagy and mitochondrial function in the context of “normal” aging, Parkinson’s disease, amyotrophic lateral sclerosis and Alzheimer’s disease.