bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Deforestation Pattern Changes (2000–2017) in the Central Carpathians: A Gray-Level Co-Occurrence Matrix and Fractal Analysis Approach
Authors: Ciobotaru Ana-Maria , Andronache Ion , Ahammer Helmut , Jelinek Herbert F. , Radulovic Marko , Pintilii Radu-Daniel , Peptenatu Daniel , Drăghici Cristian-Constantin , Simion Adrian-Gabriel , Papuc Răzvan-Mihail , Marin Marian , Radu Roxana-Andreea , Grecu Alexandra , Gruia Andreea Karina , Loghin Ioan-Vlad , Fensholt Rasmus
Published: April 2019

The paper explores the distribution of tree cover and deforested areas in the Central Carpathians in the central-east part of Romania, in the context of the anthropogenic forest disturbances and sustainable forest management. The study aims to evaluate the spatiotemporal changes in deforested areas due to human pressure in the Carpathian Mountains, a sensitive biodiverse European ecosystem. We used an analysis of satellite imagery with Landsat-7 Enhanced Thematic Mapper Plus (Landsat-7 ETM+) from the University of Maryland (UMD) Global Forest Change (GFC) dataset. The workflow started with the determination of tree cover and deforested areas from 2000–2017, with an overall accuracy of 97%. For the monitoring of forest dynamics, a Gray-Level Co-occurrence Matrix analysis (Entropy) and fractal analysis (Fractal Fragmentation-Compaction Index and Tug-of-War Lacunarity) were utilized. The increased fragmentation of tree cover (annually 2000–2017) was demonstrated by the highest values of the Fractal Fragmentation-Compaction Index, a measure of the degree of disorder (Entropy) and heterogeneity (Lacunarity). The principal outcome of the research reveals the dynamics of disturbance of tree cover and deforested areas expressed by the textural and fractal analysis. The results obtained can be used in the future development and adaptation of forestry management policies to ensure sustainable management of exploited forest areas.
Fire and Forest Management in Montane Forests of the Northwestern States and California, USA
Authors: Allen Iris , Chhin Sophan , Zhang Jianwei
Published: April 2019

We reviewed forest management in the mountainous regions of several northwestern states and California in the United States and how it has impacted current issues facing these forests. We focused on the large-scale activities like fire suppression and logging which resulted in landscape level changes. We divided the region into two main forests types; wet, like the forests in the Pacific Northwest, and dry, like the forests in the Sierra Nevada and Cascade ranges. In the wet forests, the history of intensive logging shaped the current forest structure, while fire suppression played a more major role in the dry forests. Next, we looked at how historical management has influenced new forest management challenges, like catastrophic fires, decreased heterogeneity, and climate change. We then synthesized what current management actions are performed to address these issues, like thinning to reduce fuels or improve structural heterogeneity, and restoration after large-scale disturbances. Lastly, we touch on some major policies that have influenced changes in management. We note a trend towards ecosystem management that considers a forest’s historical disturbance regime. With expected climate induced changes in fire frequency, it is suggested that fuel treatments be implemented in dry forests to ensure an understory fire regime is restored in these forest systems. With respect to wet forests in this region, it is suggested that there is still a place for stand-replacing fire regimes. However, these forests will require structural changes incorporating heterogeneity to improve their resiliency and health.
Routing-Proofness in Congestion-Prone Networks
Authors: Juarez Ruben , Wu Michael
Published: April 2019

We consider the problem of sharing the cost of connecting a large number of atomless agents in a network. The centralized agency elicits the target nodes that agents want to connect, and charges agents based on their demands. We look for a cost-sharing mechanism that satisfies three desirable properties: efficiency which charges agents based on the minimum total cost of connecting them in a network, stand-alone core stability which requires charging agents not more than the cost of connecting by themselves directly, and limit routing-proofness which prevents agents from profitable reporting as several agents connecting from A to C to B instead of A to B. We show that these three properties are not always compatible for any set of cost functions and demands. However, when these properties are compatible, a new egalitarian mechanism is shown to satisfy them. When the properties are not compatible, we find a rule that meets stand-alone core stability, limit routing-proofness and minimizes the budget deficit.
Delayed Feeding Alters Transcriptional and Post-Transcriptional Regulation of Hepatic Metabolic Pathways in Peri-Hatch Broiler Chicks
Authors: Hicks Julie A. , Porter Tom E. , Sunny Nishanth E. , Liu Hsiao-Ching
Published: April 2019

Hepatic fatty acid oxidation of yolk lipoproteins provides the main energy source for chick embryos. Post-hatching these yolk lipids are rapidly exhausted and metabolism switches to a carbohydrate-based energy source. We recently demonstrated that many microRNAs (miRNAs) are key regulators of hepatic metabolic pathways during this metabolic switching. MiRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression in most eukaryotes. To further elucidate the roles of miRNAs in the metabolic switch, we used delayed feeding for 48 h to impede the hepatic metabolic switch. We found that hepatic expression of several miRNAs including miR-33, miR-20b, miR-34a, and miR-454 was affected by delaying feed consumption for 48 h. For example, we found that delayed feeding resulted in increased miR-20b expression and conversely reduced expression of its target FADS1, an enzyme involved in fatty acid synthesis. Interestingly, the expression of a previously identified miR-20b regulator FOXO3 was also higher in delayed fed chicks. FOXO3 also functions in protection of cells from oxidative stress. Delayed fed chicks also had much higher levels of plasma ketone bodies than their normal fed counterparts. This suggests that delayed fed chicks rely almost exclusively on lipid oxidation for energy production and are likely under higher oxidative stress. Thus, it is possible that FOXO3 may function to both limit lipogenesis as well as to help protect against oxidative stress in peri-hatch chicks until the initiation of feed consumption. This is further supported by evidence that the FOXO3-regulated histone deacetylase (HDAC2) was found to recognize the FASN (involved in fatty acid synthesis) chicken promoter in a yeast one-hybrid assay. Expression of FASN mRNA was lower in delayed fed chicks until feed consumption. The present study demonstrated that many transcriptional and post-transcriptional mechanisms, including miRNA, form a complex interconnected regulatory network that is involved in controlling lipid and glucose molecular pathways during the metabolic transition in peri-hatch chicks.
Prediction of Long Non-Coding RNAs Based on Deep Learning
Authors: Liu Xiu-Qin , Li Bing-Xiu , Zeng Guan-Rong , Liu Qiao-Yue , Ai Dong-Mei
Published: April 2019

With the rapid development of high-throughput sequencing technology, a large number of transcript sequences have been discovered, and how to identify long non-coding RNAs (lncRNAs) from transcripts is a challenging task. The identification and inclusion of lncRNAs not only can more clearly help us to understand life activities themselves, but can also help humans further explore and study the disease at the molecular level. At present, the detection of lncRNAs mainly includes two forms of calculation and experiment. Due to the limitations of bio sequencing technology and ineluctable errors in sequencing processes, the detection effect of these methods is not very satisfactory. In this paper, we constructed a deep-learning model to effectively distinguish lncRNAs from mRNAs. We used k-mer embedding vectors obtained through training the GloVe algorithm as input features and set up the deep learning framework to include a bidirectional long short-term memory model (BLSTM) layer and a convolutional neural network (CNN) layer with three additional hidden layers. By testing our model, we have found that it obtained the best values of 97.9%, 96.4% and 99.0% in F1score, accuracy and auROC, respectively, which showed better classification performance than the traditional PLEK, CNCI and CPC methods for identifying lncRNAs. We hope that our model will provide effective help in distinguishing mature mRNAs from lncRNAs, and become a potential tool to help humans understand and detect the diseases associated with lncRNAs.
FARMAPRICE: A Pharmacogenetic Clinical Decision Support System for Precise and Cost-Effective Therapy
Authors: Roncato Rossana , Dal Cin Lisa , Mezzalira Silvia , Comello Francesco , De Mattia Elena , Bignucolo Alessia , Giollo Lorenzo , D’Errico Simone , Gulotta Antonio , Emili Luca , Carbone Vincenzo , Guardascione Michela , Foltran Luisa , Toffoli Giuseppe , Cecchin Erika
Published: April 2019

Pharmacogenetic (PGx) guidelines for the precise dosing and selection of drugs remain poorly implemented in current clinical practice. Among the barriers to the implementation process is the lack of clinical decision support system (CDSS) tools to aid health providers in managing PGx information in the clinical context. The present study aimed to describe the first Italian endeavor to develop a PGx CDSS, called FARMAPRICE. FARMAPRICE prototype was conceived for integration of patient molecular data into the clinical prescription process in the Italian Centro di Riferimento Oncologico (CRO)-Aviano Hospital. It was developed through a coordinated partnership between two high-tech companies active in the computerization of the Italian healthcare system. Introducing FARMAPRICE into the clinical setting can aid physicians in prescribing the most efficacious and cost-effective pharmacological therapy available.
A Hybrid Spatial Multi-Criteria Evaluation Method for Mapping Landslide Susceptible Areas in Kullu Valley, Himalayas
Authors: Meena Sansar Raj , Mishra Brijendra Kumar , Tavakkoli Piralilou Sepideh
Published: April 2019

In this paper we report our results from analysing a hybrid spatial multi-criteria evaluation (SMCE) method for generating landslide susceptibility mapping (LSM). This study is the first of its kind in the Kullu valley, Himalayas. We used eight related geospatial conditioning factors from three main groups: geological, morphological and topographical factors. Our landslide inventory dataset has a total of 149 GPS points of landslide locations, collected based on a field survey in July 2018. The relationships between landslide locations and conditioning factors were determined using the GIS-based statistical methods of frequency ratio (FR), multi-criteria decision-making (MCDM) and the integration method of hybrid SMCE. We compared the performance of applied methods by dividing the inventory into testing (70%) and validation (30%) datasets. The area under the curve (AUC) was used to validate the results. The integration method of hybrid SMCE gave the highest accuracy rate (0.910) compared to the other two methods, with 0.797 and 0.907 accuracy rates for the analytical hierarchy process (AHP) and FR, respectively. The applied methodologies are easily transferable to other areas, and the resulting landslide susceptibility maps (LSMs) can be useful for risk mitigation and development planning purposes in the Kullu valley, Himalayas.
Detection of Boulders in Side Scan Sonar Mosaics by a Neural Network
Authors: Feldens Peter , Darr Alexander , Feldens Agata , Tauber Franz
Published: April 2019

Boulders provide ecologically important hard grounds in shelf seas, and form protected habitats under the European Habitats Directive. Boulders on the seafloor can usually be recognized in backscatter mosaics due to a characteristic pattern of high backscatter intensity followed by an acoustic shadow. The manual identification of boulders on mosaics is tedious and subjective, and thus could benefit from automation. In this study, we train an object detection framework, RetinaNet, based on a neural network backbone, ResNet, to detect boulders in backscatter mosaics derived from a sidescan-sonar operating at 384 kHz. A training dataset comprising 4617 boulders and 2005 negative examples similar to boulders was used to train RetinaNet. The trained model was applied to a test area located in the Kriegers Flak area (Baltic Sea), and the results compared to mosaic interpretation by expert analysis. Some misclassification of water column noise and boundaries of artificial plough marks occurs, but the results of the trained model are comparable to the human interpretation. While the trained model correctly identified a higher number of boulders, the human interpreter had an advantage at recognizing smaller objects comprising a bounding box of less than 7 × 7 pixels. Almost identical performance between the best model and expert analysis was found when classifying boulder density into three classes (0, 1–5, more than 5) over 10,000 m2 areas, with the best performing model reaching an agreement with the human interpretation of 90%.
Correlation between Helicobacter pylori Infection and Gastric Atrophy Examined in the Sera of Mongolian People
Authors: Amgalanbaatar Avarzed , Shimomura Hirofumi , Sugano Kentaro , Bira Tsatsralt-Od , Okamoto Hiroaki , Hirai Yoshikazu
Published: April 2019

Serum specimens obtained from 680 individuals were examined to measure the amounts of pepsinogens 1 and 2, anti-CagA antibodies, and anti-Helicobacter pylori antibodies. We examined sera obtained from 610 Mongolian individuals living in the capital city, Ulaanbaatar. Seventy serum specimens were collected from Japanese people who were health-screened: These were stored at the gastroenterology laboratory of Jichi Medical University. The sera of the Japanese people were used as a control specimen. Two enzyme-linked immunosorbent assay (ELISA) kits, an E-plate ELISA kit from Eiken Chemical Co., Ltd. (Tokyo, Japan), and a Biohit ELISA kit from Biohit Oyj (Helsinki, Finland), were used for the detection of anti-H. pylori IgG antibodies in the sera of the 610 Mongolian people. An ELISA kit EIA-4138 from DRG Instruments GmbH (Germany) was used for the detection of anti-CagA IgG antibodies in the serum specimens. Serum pepsinogens were detected by an ELISA kit from Biohit Oyj. Of the 610 serum specimens, 385 specimens tested positive for the detection of anti-H. pylori antibodies using the two ELISA kits, and 47 tested negative. For the detection of anti-H. pylori antibodies by the Biohit ELISA kit, 560 and 50 specimens were positive and negative, respectively. The ratio of serum pepsinogen 1/2 was statistically lower (p < 0.0001) in the H. pylori-positive (560 specimens) than in the H. pylori-negative (50 specimens) specimens. However, the levels of serum pepsinogen 1 had no statistical significance (p = 0.465) between the specimens of the H. pylori-positive and -negative specimens. The ratio of serum pepsinogen 1/2 was 6.74 ± 0.12 in the H. pylori-positive specimens, whereas the ratio of serum pepsinogen 1/2 was 12.69 ± 1.02 in the H. pylori-negative specimens. This study demonstrated the high prevalence of H. pylori infection in Mongolian people, including young generations, and the people infected with H. pylori possessed low pepsinogen 1/2 ratios, indicating atrophic gastritis. The serological examinations by the two ELISA kits did not consistently reflect the prevalence of H. pylori infection in Mongolian people.
The Effect of Health on Labour Supply of Rural Elderly People in China—An Empirical Analysis Using CHARLS Data
Authors: Jiang Jinqi , Huang Wanzhen , Wang Zhenhua , Zhang Guangsheng
Published: April 2019

In China, due to decades of the ‘one-child policy’ and continuous rural-urban labour migration, real population aging in rural areas is increasing more quickly than in urban areas, and the labour inputs in agricultural production are becoming ever more dependent on the elderly. Using CHARLS data, we examine the effect of health on the labour supply of rural elderly people. We construct a latent health stock index (LHSI) to eliminate measurement bias and then use this one-period lagged LHSI and the Heckman two-stage and the Bourguignon-Fournier-Gurand two-stage method to deal with the simultaneous causality of health and labour decisions and sample selectivity in model estimation. The results show that, in the overall level, the labour force participation and work time of rural elderly people increase significantly with the improvement of health. These effects on the males are sharply greater than on the females and are enhanced with age. In the subdivided agricultural and non-agricultural labour supply, health improvement is positively related with labour force participation of rural elderly and brings an employment allocation from agricultural section to non-agricultural section, especially on the males. However, as the work time, these relations are insignificant and invariant with gender and age.
Considerations for Designing Context-Aware Mobile Apps for Mental Health Interventions
Authors: Miralles Ignacio , Granell Carlos
Published: April 2019

This work identifies major areas of knowledge and proposes a set of relevant dimensions by area that must be taken into account in the design and delivery of context-aware mobile applications for mental health interventions. We argue that much of the related research has focused only on a few dimensions, paying little or no attention to others and, most importantly, to potential relationships between them. Our belief is that the improvement of the effectiveness of mobile interventions to support mental health necessarily implies that developers and therapists comprehensively consider the interaction between the proposed dimensions. Taking as a starting point the three areas of knowledge (Technology, Context, and Mental Health), we re-examine each area to identify relevant dimensions, discuss the relationships between them and finally draw a series of considerations. The resulting considerations can help therapists and developers to devise, design, and generate custom mobile applications in a way that increases the motivation and engagement of patients and, therefore, the effectiveness of psychological treatments.
Reply to Accelerated Silicosis—An Emerging Epidemic Associated with Engineered Stone. Comment on Leso, V. et al. Artificial Stone-Associated Silicosis: A Systematic Review. Int. J. Environ. Res. Public Health 2019, 16(4), 568, doi:10.3390/ijerph16040568
Authors: Leso Veruscka , Fontana Luca , Romano Rosaria , Gervetti Paola , Iavicoli Ivo
Published: April 2019

Our systematic review on artificial stone (AS)-associated silicosis and the related comment by Edwards underline the urgency to define effective strategies to assess and manage the risk of exposure to silica in workers involved in AS job tasks. Case screening programs may be important to fully understand the extent of the silicosis epidemic associated with AS and point out critical issues in workplace settings/practices that, by contributing to higher respirable crystalline exposure, favor the disease manifestation. This information may guide the identification of the most appropriate preventive measures in workplaces, especially regarding the administration of updated training and information courses, the definition of good working practices, and the application of targeted health surveillance programs. However, considering the recent epidemiological data and the severity of AS-associated silicosis, it would be appropriate (according to the hierarchy of control strategy) to also consider the application of more stringent prevention measures, such as a safety-by-design approach to the chemical formulation of the AS. Overall, the implementation of the aforementioned preventive measures should ensure an effective control of the current silicosis epidemic and, at the same time, prevent the development of new disease cases in the near future.
Determining the Enablers and Barriers for the Adoption of Clean Cookstoves in the Middle Belt of Ghana—A Qualitative Study
Authors: Agbokey Francis , Dwommoh Rebecca , Tawiah Theresa , Ae-Ngibise Kenneth Ayuurebobi , Mujtaba Mohammed Nuhu , Carrion Daniel , Ali Abdulai Martha , Afari-Asiedu Samuel , Owusu-Agyei Seth , Asante Kwaku Poku , Jack Darby W.
Published: April 2019

Despite its benefits and espousal in developed counties, the adoption of clean cookstoves is reportedly low in less developed countries, especially in Sub–Saharan Africa. This qualitative study aimed at exploring and documenting the enablers and barriers for adoption of clean cookstove in the middle belt of Ghana. The findings showed convenience of clean cookstove use, reduced firewood usage, less smoke emission and associated health problems resulting from indoor air pollution and time for firewood gathering and cooking, good smell and taste of food as enabling factors for clean cookstove adoption. Factors such as safety, financial constraint (cost), non-availability of spare parts on the open market to replace faulty stove accessories, stove size and household size were the potential barriers to clean cookstove adoption. These findings help us to understand the factors promoting and inhibiting the adoption of clean cook stoves, especially in rural settings.
Childhood Obesity Prevention in Africa: A Systematic Review of Intervention Effectiveness and Implementation
Authors: Klingberg Sonja , Draper Catherine E. , Micklesfield Lisa K. , Benjamin-Neelon Sara E. , van Sluijs Esther M. F.
Published: April 2019

Childhood obesity is of increasing concern in many parts of Africa. We conducted a systematic search and review of published literature on behavioural childhood obesity prevention interventions. A literature search identified peer-reviewed literature from seven databases, and unindexed African journals, including experimental studies targeting children age 2–18 years in African countries, published in any language since 1990. All experimental designs were eligible; outcomes of interest were both behavioural (physical activity, dietary behaviours) and anthropometric (weight, body mass index, body composition). We also searched for process evaluations or other implementation observations. Methodological quality was assessed; evidence was synthesised narratively as a meta-analysis was not possible. Seventeen articles describing 14 interventions in three countries (South Africa, Tunisia and Uganda) were included. Effect scores indicated no overall effect on dietary behaviours, with some beneficial effects on physical activity and anthropometric outcomes. The quality of evidence was predominantly weak. We identified barriers and facilitators to successful interventions, and these were largely resource-related. Our systematic review highlights research gaps in targeting alternative settings to schools, and younger age groups, and a need for more rigorous designs for evaluating effectiveness. We also recommend process evaluations being used more widely.
Highway Alignment Optimization: An Integrated BIM and GIS Approach
Authors: Zhao Linlin , Liu Zhansheng , Mbachu Jasper
Published: April 2019

Highway infrastructure plays an important role in assuring the proper function of the nation’s transportation. Highway alignment is an essential part of the highway planning and design phase, which has significant effects on the surroundings. Identifying optimal highway routes while using traditional methods requires significant time, cost, and effort, since it requires a comprehensive assessment of multiple factors, such as cost and environmental impacts. This study proposes an approach for managing highway alignment in the context of a larger landscape that integrates building information modelling (BIM) and geographic information system (GIS) capabilities. To support this integration, semantic web technologies are used to integrate data on a semantic level. Moreover, the approach also uses genetic algorithms (GAs) for optimizing highway alignments. A fully automated model is developed that enables data interoperability between BIM and GIS systems and also allows for data exchange between the integration model and the optimization algorithm. The model enables the full exploitation of features of the project and its surroundings for highway alignment planning. The proposed model is also applied to a real highway project to validate its effectiveness. The visualization model of the highway project and its surroundings provides a realistic three-dimensional image that produces a comprehensive virtual environment, where the project could be effectively planned and designed. That can help to reduce design errors and miscommunication, which, in turn, reduces project risks. Moreover, geological and geographical analyses help to identify geohazards and environmentally sensitive regions. The proposed model facilitates highway alignment planning by providing a cross-disciplinary approach to close the gap between the infrastructural and geotechnical planning processes.
AICAR Induces Apoptosis and Inhibits Migration and Invasion in Prostate Cancer Cells Through an AMPK/mTOR-Dependent Pathway
Authors: Su Chia-Cheng , Hsieh Kun-Lin , Liu Po-Len , Yeh Hsin-Chih , Huang Shu-Pin , Fang Shih-Hua , Cheng Wei-Chung , Huang Kuan-Hua , Chiu Fang-Yen , Lin I-Ling , Huang Ming-Yii , Li Chia-Yang
Published: April 2019

Current clinical challenges of prostate cancer management are to restrict tumor growth and prohibit metastasis. AICAR (5-aminoimidazole-4-carbox-amide-1-β-d-ribofuranoside), an AMP-activated protein kinase (AMPK) agonist, has demonstrated antitumor activities for several types of cancers. However, the activity of AICAR on the cell growth and metastasis of prostate cancer has not been extensively studied. Herein we examine the effects of AICAR on the cell growth and metastasis of prostate cancer cells. Cell growth was performed by MTT assay and soft agar assay; cell apoptosis was examined by Annexin V/propidium iodide (PI) staining and poly ADP ribose polymerase (PARP) cleavage western blot, while cell migration and invasion were evaluated by wound-healing assay and transwell assay respectively. Epithelial–mesenchymal transition (EMT)-related protein expression and AMPK/mTOR-dependent signaling axis were analyzed by western blot. In addition, we also tested the effect of AICAR on the chemosensitivity to docetaxel using MTT assay. Our results indicated that AICAR inhibits cell growth in prostate cancer cells, but not in non-cancerous prostate cells. In addition, our results demonstrated that AICAR induces apoptosis, attenuates transforming growth factor (TGF)-β-induced cell migration, invasion and EMT-related protein expression, and enhances the chemosensitivity to docetaxel in prostate cancer cells through regulating the AMPK/mTOR-dependent pathway. These findings support AICAR as a potential therapeutic agent for the treatment of prostate cancer.
Increased Expression of Cell Surface SSEA-1 is Closely Associated with Naïve-Like Conversion from Human Deciduous Teeth Dental Pulp Cells-Derived iPS Cells
Authors: Inada Emi , Saitoh Issei , Kubota Naoko , Iwase Yoko , Murakami Tomoya , Sawami Tadashi , Yamasaki Youichi , Sato Masahiro
Published: April 2019

Stage-specific embryonic antigen 1 (SSEA-1) is an antigenic epitope (also called CD15 antigen) defined as a Lewis X carbohydrate structure and known to be expressed in murine embryonal carcinoma cells, mouse embryonic stem cells (ESCs), and murine and human germ cells, but not human ESCs/induced pluripotent stem cells (iPSCs). It is produced by α1,3-fucosyltransferase IX gene (FUT9), and F9 ECCs having a disrupted FUT9 locus by gene targeting are reported to exhibit loss of SSEA-1 expression on their cell surface. Mouse ESCs are pluripotent cells and therefore known as “naïve stem cells (NSCs).” In contrast, human ESCs/iPSCs are thought to be epiblast stem cells (EpiSCs) that are slightly more differentiated than NSCs. Recently, it has been demonstrated that treatment of EpiSCs with several reprograming-related drugs can convert EpiSCs to cells similar to NSCs, which led us to speculate that SSEA-1 may have been expressed in these NSC-like EpiSCs. Immunocytochemical staining of these cells with anti-SSEA-1 revealed increased expression of this epitope. RT-PCR analysis also confirmed increased expression of FUT9 transcripts as well as other stemness-related transcripts such as REX-1 (ZFP42). These results suggest that SSEA-1 can be an excellent marker for human NSCs.
The Analgesic Effect of Venlafaxine and Its Mechanism on Oxaliplatin-Induced Neuropathic Pain in Mice
Authors: Li Daxian , Lee Ji Hwan , Choi Chang Won , Kim Jaihwan , Kim Sun Kwang , Kim Woojin
Published: April 2019

The analgesic effect of venlafaxine (VLX), which is a selective serotonin and noradrenaline reuptake inhibitor (SNRI), has been observed on oxaliplatin-induced neuropathic pain in mice. Significant allodynia was shown after oxaliplatin treatment (6 mg/kg, i.p.); acetone and von Frey hair tests were used to assess cold and mechanical allodynia, respectively. Intraperitoneal administration of VLX at 40 and 60 mg/kg, but not 10 mg/kg, significantly alleviated these allodynia. Noradrenaline depletion by pretreatment of N-(2-Chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4, 50 mg/kg, i.p.) blocked the relieving effect of VLX (40 mg/kg, i.p.) on cold and mechanical allodynia. However, serotonin depletion by three consecutive pretreatments of para-chlorophenylalanine (PCPA, 150 mg/kg/day, i.p.) only blocked the effect of VLX on mechanical allodynia. In cold allodynia, the α2-adrenergic antagonist idazoxan (10 μg, i.t.), but not the α1-adrenergic antagonist prazosin (10 μg, i.t.), abolished VLX-induced analgesia. Furthermore, idazoxan and 5-HT3 receptor antagonist bemesetron (MDL-72222, 15 μg, i.t.), but not prazosin or mixed 5-HT1, 2 receptor antagonist methysergide (10 μg, i.t.), abolished VLX-induced analgesia in mechanical allodynia. In conclusion, 40 mg/kg of VLX treatment has a potent relieving effect against oxaliplatin-induced neuropathic pain, and α2-adrenergic receptor, and both α2-adrenergic and 5-HT3 receptors are involved in this effect of VLX on cold and mechanical allodynia, respectively.
Endoplasmic Reticulum Stress: A Critical Molecular Driver of Endothelial Dysfunction and Cardiovascular Disturbances Associated with Diabetes
Authors: Maamoun Hatem , Abdelsalam Shahenda S. , Zeidan Asad , Korashy Hesham M. , Agouni Abdelali
Published: April 2019

Physical inactivity and sedentary lifestyle contribute to the widespread epidemic of obesity among both adults and children leading to rising cases of diabetes. Cardiovascular disease complications associated with obesity and diabetes are closely linked to insulin resistance and its complex implications on vascular cells particularly endothelial cells. Endoplasmic reticulum (ER) stress is activated following disruption in post-translational protein folding and maturation within the ER in metabolic conditions characterized by heavy demand on protein synthesis, such as obesity and diabetes. ER stress has gained much interest as a key bridging and converging molecular link between insulin resistance, oxidative stress, and endothelial cell dysfunction and, hence, represents an interesting drug target for diabetes and its cardiovascular complications. We reviewed here the role of ER stress in endothelial cell dysfunction, the primary step in the onset of atherosclerosis and cardiovascular disease. We specifically focused on the contribution of oxidative stress, insulin resistance, endothelial cell death, and cellular inflammation caused by ER stress in endothelial cell dysfunction and the process of atherogenesis.
Effect of Co-Inoculation with Saccharomyces cerevisiae and Lactic Acid Bacteria on the Content of Propan-2-ol, Acetaldehyde and Weak Acids in Fermented Distillery Mashes
Authors: Pielech-Przybylska Katarzyna , Balcerek Maria , Ciepielowski Grzegorz , Pacholczyk-Sienicka Barbara , Albrecht Łukasz , Dziekońska-Kubczak Urszula , Bonikowski Radosław , Patelski Piotr
Published: April 2019

The qualitative and quantitative composition of volatile compounds in fermented distillery mash determines the quality of the obtained distillate of agricultural origin (i.e., raw spirit) and the effectiveness of further purification steps. Propan-2-ol (syn. isopropyl alcohol), due to its low boiling point, is difficult to remove by rectification. Therefore, its synthesis needs to be limited during fermentation by Saccharomyces cerevisiae yeast, while at the same time controlling the levels of acetaldehyde and acetic acid, which are likewise known to determine the quality of raw spirit. Lactic acid bacteria (LAB) are a common but undesirable contaminant in distillery mashes. They are responsible for the production of undesirable compounds, which can affect synthesis of propan-2-ol. Some bacteria strains are able to synthesize isopropyl alcohol. This study therefore set out to investigate whether LAB with S. cerevisiae yeast are responsible for conversion of acetone to propan-2-ol, as well as the effects of the amount of LAB inoculum and fermentation parameters (pH and temperature) on the content of isopropyl alcohol, acetaldehyde, lactic acid and acetic acid in fermented mashes. The results of NMR and comprehensive two-dimensional gas chromatography coupled with time of flight mass spectrometry (GC × GC-TOF MS) analysis confirmed the ability of the yeast and LAB strains to metabolize acetone via its reduction to isopropyl alcohol. Efficient fermentation of distillery mashes was observed in all tested mashes with an initial LAB count of 3.34–6.34 log cfu/mL, which had no significant effect on the ethanol content. However, changes were observed in the contents of by-products. Lowering the initial pH of the mashes to 4.5, without and with LAB (3.34–4.34 log cfu/mL), resulted in a decrease in propan-2-ol and a concomitant increase in acetaldehyde content, while a higher pH (5.0 and 5.5) increased the content of propan-2-ol and decreased acetaldehyde content. Higher temperature (35 °C) promoted propan-2-ol synthesis and also resulted in increased acetic acid content in the fermented mashes compared to the controls. Moreover, the acetic acid content rose with increases in the initial pH and the initial LAB count.
Ameliorative Effect of Spinach on Non-Alcoholic Fatty Liver Disease Induced in Rats by a High-Fat Diet
Authors: Elvira-Torales Laura Inés , Martín-Pozuelo Gala , González-Barrio Rocío , Navarro-González Inmaculada , Pallarés Francisco-José , Santaella Marina , García-Alonso Javier , Sevilla Ángel , Periago-Castón María Jesús
Published: April 2019

The purpose of this work was to evaluate the effect of dietary carotenoids from spinach on the inflammation and oxidative stress biomarkers, liver lipid profile, and liver transcriptomic and metabolomics profiles in Sprague–Dawley rats with steatosis induced by a high-fat diet. Two concentrations of spinach powder (2.5 and 5%) were used in two types of diet: high-fat (H) and standard (N). Although rats fed diet H showed an accumulation of fat in hepatocytes, they did not show differences in the values of adiponectin, tumor necrosis factor alpha (TNF-α), and oxygen radical absorption (ORAC) in plasma or of isoprostanes in urine compared with animals fed diet N. The consumption of spinach and the accumulation of α and β carotenes and lutein in the liver was inversely correlated with serum total cholesterol and glucose and the content of hepatic cholesterol, increasing monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA) and reducing cholesterol in the livers of rats fed diet H and spinach. In addition, changes in the expression of genes related to the fatty liver condition occurred, and the expression of genes involved in the metabolism of fatty acids and cholesterol increased, mainly through the overexpression of peroxisome proliferator activated receptors (PPARs). Related to liver metabolites, animals fed with diet H showed hypoaminoacidemia, mainly for the glucogenic aminoacids. Although no changes were observed in inflammation and oxidative stress biomarkers, the consumption of spinach modulated the lipid metabolism in liver, which must be taken into consideration during the dietary treatment of steatosis.
Fritillariae Thunbergii Bulbus: Traditional Uses, Phytochemistry, Pharmacodynamics, Pharmacokinetics and Toxicity
Authors: Li Hong , Hung Andrew , Li Mingdi , Yang Angela Wei Hong
Published: April 2019

Fritillariae Thunbergii Bulbus (FTB) has been widely used as an antitussive herb for thousands of years in China. However, FTB’s traditional uses, chemical compounds and pharmacological activities have not been systematically reviewed. This study aimed to review its traditional uses, phytochemistry, pharmacodynamics, pharmacokinetics and toxicity. We searched the Encyclopedia of Traditional Chinese Medicine to explore the historical records which indicate that it acts to clear heat, resolve phlegm, relieve cough, remove toxicity and disperse abscesses and nodules. We searched 11 databases to identify potential phytochemical or pharmacological studies. Characteristics of its chemical constituents, pharmacological effects, pharmacokinetic and toxicity were descriptively summarized. A total of 9706 studies were identified and 83 of them were included. As a result, 134 chemical constituents were identified, including 26 alkaloids, 29 compounds found in essential oils, 13 diterpenoids, two carbohydrates, two sterols, 18 amino acids, six nucleosides, four nucleobases, four fatty acids, three lignans, and 27 elements. Thirteen pharmacological effects of FTB were identified, including anti-cancer, tracheobronchial relaxation, antitussive, expectorant, anti-muscarinic, anti-inflammation, anti-thyroid, regulation of blood rheology, antiulcer, anti-diarrhea, pain suppression, antioxidation and neuroprotection. These pharmacological activities may be mainly attributed to the alkaloids in FTB. Further phytochemical, pharmacological and network pharmacological studies are recommended.
Overexpression of PtDXS Enhances Stress Resistance in Poplars
Authors: Wei Hui , Movahedi Ali , Xu Chen , Sun Weibo , Almasi Zadeh Yaghuti Amir , Wang Pu , Li Dawei , Zhuge Qiang
Published: April 2019

1-Deoxy-d-xylulose-5-phosphate synthase (DXS) is the rate-limiting enzyme in the plastidial methylerythritol phosphate pathway (MEP). In this study, PtDXS (XM_024607716.1) was isolated from Populus trichocarpa. A bioinformatics analysis revealed that PtDXS had high homology with the DXSs of other plant species. PtDXS expression differed among plant tissues and was highest in young leaves and lowest in roots. The recombinant protein was produced in Escherichia coli BL21 (DE3), purified, and its activity evaluated. The purified protein was capable of catalyzing the formation of 1-deoxy-d-xylulose-5-phosphate (DXP) from glyceraldehyde-3-phosphate and pyruvate. A functional color assay in E. coli harboring pAC-BETA indicated that PtDXS encodes a functional protein involved in the biosynthesis of isoprenoid precursors. The treatment of P. trichocarpa seedlings with 200 μM abscisic acid (ABA), 200 mM NaCl, 10% polyethylene glycol6000, and 2 mM H2O2 resulted in increased expression of PtDXS. The ABA and gibberellic acid contents of the transgenic lines (Poplar Nanlin 895) were higher than wild types, suggesting that DXS is important in terpenoid biosynthesis. Overexpression of PtDXS enhanced resistance to S. populiperda infection. Furthermore, the transgenic lines showed decreased feeding by Micromelalopha troglodyta, supporting the notion that PtDXS is a key enzyme in terpenoid biosynthesis.
Silver and Copper Nanoparticles—An Alternative in Future Mastitis Treatment and Prevention?
Authors: Kalińska Aleksandra , Jaworski Sławomir , Wierzbicki Mateusz , Gołębiewski Marcin
Published: April 2019

Nowadays, mastitis is one of the biggest problems in breeding dairy cattle. Treatment of this disease with conventional antibiotics is ineffective because many pathogens are resistant. Researchers have therefore been forced to look for new solutions, and metal nanoparticles (NPs) have been found to be the most appropriate agents. This study uses commercially available silver (AgNPs) and copper (CuNPs) nanoparticles and synthetized silver–copper nanoparticles (AgCuNPs) to evaluate the effect of these NPs on human and bovine mammary cells. The effect of AgNPs, CuNPs, and AgCuNPs on pathogen species commonly involved in udder inflammation (e.g., Staphylococcus aureus and Escherichia coli) was also established. The results show that commercially available NPs were of good quality and did not have a toxic effect on mammary gland tissue. AgNPs, CuNPs, and AgCuNPs also influenced or decreased the viability of pathogens. Therefore, the presented data suggest that metal NPs could be used in mastitis prevention and treatment in the future. However, the presented preliminary results require further in vivo analysis.
Photo-Triggered Reversible Phase Transfer of Azobenzene-Based Ionic Liquid Surfactants between Oil and Water
Authors: Li Zhiyong , Feng Ying , Yuan Xiaoqing , Wang Huiyong , Zhao Yuling , Wang Jianji
Published: April 2019

The reversible phase transfer of compounds between two immiscible liquid phases has many applications in a wide range of fields, and ionic liquids have been widely used as potential functional solvents and catalysts. However, photo-triggered reversible phase transfer of ionic liquids between the organic phase and water phase has not been reported so far. In the present work, the reversible phase transfer of six kinds of azobenzene-based ionic liquid surfactants between the organic phase and water phase is investigated by alternative irradiation of UV and visible light. Factors affecting the transfer efficiency, such as chemical structure and concentration of the ionic liquid surfactants, equilibrium photo-isomerization degree, and the aggregation state of ionic liquid surfactants are investigated in detail. It is shown that transfer efficiency greater than 89% was achieved under optimal conditions, equilibrium photo-isomerization degree of the ionic liquid surfactants is the main factor to determine their transfer efficiencies, and the aggregation of cis-isomers is not beneficial for the transfer.
Dietary Adherence of Saudi Males to the Saudi Dietary Guidelines and Its Relation to Cardiovascular Diseases: A Preliminary Cross-Sectional Study
Authors: Alkhaldy Areej Ali , Alamri Reem Saleh , Magadmi Rozana Khalid , Elshini Nrvana Yasser , Hussein Rania Abd El Hamid , Alghalayini Kamal Waheeb
Published: April 2019

Cardiovascular disease (CVD) is a major public health problem in Saudi Arabia. Dietary intake plays a major role in CVD incidence; however, the dietary intake status in Saudi nationals with CVD is unknown. We aimed to investigate whether the dietary patterns of Saudi males, using the Saudi dietary guidelines adherence score, in parallel with the measurement of a selective number of cardiovascular disease-related biomarkers, are contributing factors to CVD risk. Demographics, dietary adherence score, and blood biomarker levels were collected for 40 CVD patients and forty non-CVD patients. Fasting blood glucose (p = 0.006) and high-density lipoprotein levels (p = 0.03) were significantly higher in CVD patients. The adherence score to the Saudi dietary guidelines was not significantly different between the CVD and non-CVD patients; however, the specific adherence scores of fruit (p = 0.02), olive oil (p = 0.01), and non-alcoholic beer (p = 0.02) were significantly higher in the non-CVD patients. The differences in CVD family history (p = 0.02) and adherence scores to specific groups/foods between the CVD and non-CVD patients may contribute to CVD risk in Saudi males. However, as the sample size of this study was small, further research is required to validate these findings.
Seasonal Changes in the Intertidal and Subtidal Algal Communities of Extremely and Moderately Polluted Coastal Regions of Sanya Bay (Hainan Island, China)
Authors: Titlyanov Eduard A. , Titlyanova Tamara V. , Huang Hui , Scriptsova Anna V. , Xu Huili , Li Xiubao
Published: April 2019

At the end of the rainy season in 2016 and at the end of the dry season in 2017, we conducted a floristic study of marine macrophytic algae in the intertidal and subtidal zones in moderately and heavily polluted areas at Luhuitou reef, Sanya Bay, Hainan Island, China. A total of 109 species of marine macrophytes were found during these samplings. At the end of the rainy season, 72 species of macrophytes (50% reds, 19% browns, and 31% greens) were found. At the end of the dry season, we found and identified 92 species of macrophytes (46% reds, 20% browns, and 34% greens). Seasonal changes in species diversity, species composition, and the structure of algal communities at differently polluted sites exhibited common features as well as specific characteristics. By the end of the dry season, the diversity of macroalgal species was increased, and the composition of dominant and accompanying species of macrophytes in polydominant communities was changed in moderately and heavily polluted areas. Seasonal changes in the marine flora of differently polluted areas were characterized by specific features as follows: Less changes in species diversity of heavily polluted area compared with moderately polluted area during the change from the rainy season to the dry season; significant increase in the biomass of green algae and their projective coverage in the middle and low intertidal zones of heavily polluted sites in the dry season; and the increase in the numbers of mono- and bidominant communities in the middle and low intertidal zones of heavily polluted sites by the end of the dry season.
Numerical Investigation into the Two-Phase Convective Heat Transfer within the Hold of an Oil Tanker Subjected to a Rolling Motion
Authors: Yu Guojun , Jia Sheng , Geng Yanting
Published: April 2019

A crude oil tanker usually encounters a rolling motion during sea transportation, which leads to rotational movement and sometimes a sloshing of the liquid hold. This rolling-induced body motion seriously affects the thermal and hydraulic behavior of the liquid hold, which then affects the heating process and heat preservation of the tanker. Clarification of the involved thermal and hydraulic characteristics is the basic requirement for establishment of a scientific heating scheme and heat preservation method. A two-phase 3D model considering the free liquid surface and non-Newtonian behavior of the fluid was established for the thermal calculation of the liquid holds in oil tankers. The thermal and hydraulic characteristics of the liquid hold were investigated under different combinations of dimensionless parameters, and the combined effect of rolling and fluid non-Newtonian behavior was investigated. It was found that rolling intensifies the heat transfer based on the combination of the Richardson number ( R i ) and the rotation-strength number ( ω * ), and non-Newtonian behavior of the fluid effectively affects the heat transfer in a rolling motion. This research is expected to provide a reference for design and optimization of the heating and heat preservation method for oil tanker operation.