bluuebunny/crossref_metadata_2025 huggingface dataset:
Human leukocyte antigen alloimmunization in a randomized trial of amustaline/glutathione pathogen‐reduced red cells in complex cardiac surgery patients
Authors: Norris Philip J. , Stone Mars , Di Germanio Clara , Balasko Brendan , Kaidarova Zhanna , Friend Henry , Varrone Jeanne , Corash Laurence , Mufti Nina , Benjamin Richard J.
Published: January 2025

Background

Although alloimmunization risk of pathogen‐reduced (PR) platelets has been studied, the risk has not been reported with PR red blood cells (RBCs).

Study Design and Methods

In a Phase III, randomized, controlled trial (Red Cell Pathogen Inactivation), cardiac or thoracic‐aorta surgery patients were randomized to transfusion with amustaline/glutathione PR versus conventional RBCs. Pre‐transfusion and Day 28 samples were evaluated for Human leukocyte antigen (HLA) Class I and Class II antibodies at low, medium, and high cutoff values.

Results

The HLA alloimmunization analysis included 114 participants (53% female) in the PR and 113 (51% female) in the conventional RBC arms. In a modified intention‐to‐treat analysis, 13.7% (N = 29) and 7.2% (N = 15) developed new high‐level HLA Class I or Class II antibodies, respectively; however, there was no signal that PR‐RBCs affected the rate of HLA Class I (odds ratio (OR) 1.3 [95% confidence interval (CI) 0.62–2.9]) or Class II antibody formation (OR 0.99 [95% CI 0.35–2.8]). Female transfusion recipients had higher risk of developing new high‐level HLA Class I antibodies (OR 12.0 [95% CI 3.5–40.9]) and Class II antibodies (OR 5.0 [95% CI 1.4–17]). The mean number of RBC (5.5 vs. 3.6 units, p = 0.018) and platelet (1.8 vs. 1.1 units, p = 0.043) transfusions was higher in subjects with new high‐level HLA Class II antibodies.

Discussion

Receipt of amustaline/glutathione PR‐RBC units did not affect HLA alloimmunization risk. Female sex and number of RBC and platelet transfusions were risk factors for the development of new high‐level HLA Class I and Class II antibodies.
Adjuvant Chemotherapy Is Associated With Prolonged Survival Time in Small‐Breed Dogs Undergoing Amputation for Appendicular Osteosarcoma
Authors: Zanardi Stefano , Sabattini Silvia , Rossi Federica , Rossanese Matteo , Buracco Paolo , Montinaro Vincenzo , Aralla Marina , Dentini Alfredo , Pizzi Elisa , Volpe Enrico , Tremolada Giovanni , Marconato Laura
Published: January 2025

Adjuvant chemotherapy is a well‐established treatment for large‐breed dogs with appendicular osteosarcoma; however, it is unclear if it improves outcomes in small‐breed dogs due to limited focused studies. This retrospective study aimed to investigate the oncologic outcomes of dogs weighting less than 15 kg with appendicular osteosarcoma that underwent curative resection with or without postoperative adjuvant chemotherapy. Endpoints were time to distant progression (TTDP) and overall survival (OS). Medical records from multiple institutions were reviewed, and 43 dogs were included in the analysis: 17 underwent surgery alone and 26 also received adjuvant chemotherapy. The median TTDP for all dogs was 265 days, with no significant difference between treatment groups. The median OS for all dogs was 270 days, and it was significantly different between amputated dogs (150 days) and those also receiving adjuvant chemotherapy (353 days, p = 0.002). In our cohort, osteosarcoma in small breeds behaved as aggressive as in large breeds. Adjuvant chemotherapy may prolong survival. Future randomised studies are needed to provide definitive evidence on the necessity of adjuvant chemotherapy to address metastatic spread in small‐breed dogs with appendicular osteosarcoma.
An Exploration of Instructional Leadership and Distributed Leadership on Teacher Self-Efficacy and Job Satisfaction Towards School Improvement in an American Curriculum School in Dubai
Authors: Abukhalaf Siham
Published: January 2025

Many teachers do not find teaching to be a desirable profession due to various reasons such as low salaries, an overwhelming workload, lack of resources, cooperation between teachers and educational leaders, little prospects for professional growth, and insufficient gratitude for their efforts. The research paper has a dual interest; first, to investigate whether there is a relationship between instructional leadership, distributed leadership and teachers’ self-efficacy and job satisfaction. Second, whether there is a correlation between instructional leadership, distributed leadership, and a supportive school culture and teachers’ collaboration in an American school in Dubai. The study employed a mixed methods design, including two surveys (one for teachers and one for educational leaders) and semi-structured interviews with educational leaders to address the research questions. The study’s key findings suggest that teacher self-efficacy and job satisfaction are positively linked to instructional and distributed leadership. Also, the study indicates that instructional and distributed leadership have a positive association with supportive school culture and teacher collaboration. The study may inform about the importance of creating a supportive school culture and promoting teachers’ collaboration to produce better results. The study has some limitations; one of the limitations is the lack of sample size due to the time constraints that I faced; hence, this research may not represent the entire population. Future research should examine multiple schools in the United Arab Emirates to obtain accurate findings and ensure that the results are representative of the population.
Orthodontic treatment from childhood to adolescence with minimally invasive therapy correction of atypical swallowing and dental alignment. A case report
Authors: Martelli Mirko , Russomanno Wanda Lelia , Di Vecchio Stefano , Gargari Marco , Bollero Patrizio , Ottria Liliana , Dolci Alessandro , Gianfreda Francesco
Published: January 2025

This case report describes a minimally invasive orthodontic approach for a patient who was identified in childhood and followed through adolescence. The approach was intended to correct atypical swallowing, diastemas, and overjet. The treatment used two devices: the Froggy Mouth for myofunctional therapy and clear aligners for dental alignment and overjet correction.
A rare case of impacted mandibular premolar associated to dentigerous cyst and periodontal lesion clinical management and histological analysis
Authors: Gianfreda Francesco , Nucci Ludovica , Bollero Patrizio , Danieli Andrea , Palermo Andrea , Salvadori Paolo , Gargari Marco , Martelli Mirko
Published: January 2025

Background: Dental cysts, particularly dentigerous cysts (DCs), are a common pathological condition affecting the jawbone. The literature categorizes DCs based on histology and associates them with impacted teeth, often leading to complications.
However, the occurrence of DCs in the elderly and their association with mandibular premolars is not well-documented, presenting a knowledge gap in dental pathology and management.
Objective: This case report aims to present a rare clinical case of an impacted mandibular premolar associated with a dentigerous cyst and a periodontal lesion, detailing the clinical management, radiographic diagnosis, histopathologic analysis, and surgical intervention.
Case presentation: A 65-year-old male patient presented with pain and mobility in tooth 36. Clinical examination, radiographic imaging, and histological analysis were conducted to diagnose and plan the treatment—the treatment involved enucleation of the DC and extraction of the impacted tooth. Histological examination was performed using hematoxylin and eosin staining to determine the nature of the cyst.
Results: The patient’s postoperative recovery was uneventful, with complete remineralization of the site after one year. Histological findings indicated a significant inflammatory infiltrate, predominantly lymphocytes, suggesting a periodontal cyst.
The impacted mandibular premolar association with a DC in an elderly patient is rare, with the literature reporting a higher frequency of such cysts in younger demographics.
Conclusions: The case report contributes to the existing literature by highlighting the successful management of a rare case of an impacted mandibular premolar with a dentigerous cyst and periodontal lesion. It emphasizes the importance of accurate diagnosis and individualized treatment planning in managing complex dental cyst cases. It suggests a conservative approach to prevent surgical risks, especially near critical anatomical structures.
Clinical and radiographical evaluation of two agenesis alveolar ridges of upper lateral incisors. A case report with 5 years follow up
Authors: Botticelli Gianluca , Pizzolante Tommaso , Capogreco Mario , Prata Paolo , Calabrò Paolo , Severino Marco , Testa Giovanni , Rastelli Sofia
Published: January 2025

Besides the functional issues, lateral incisor agenesis represents a significant aesthetic drawback (1).
The decision about the treatment plan is complex due to the long-term success, which is not easy to achieve. It has to be discussed with the orthodontists to choose the closure, using permanent canines to replace the missing teeth, or maintaining the spaces, for example, with Maryland bridges or implants.
The main advantage of orthodontic gap closure is that this approach preserves the natural architecture of the complex and soft tissues. However, canines and premolar sizes and shapes should be adjusted to mimic the replaced teeth, eventually resorting to odontoplasty or veneers (13).
This article aims to show clinical protocols and procedures for the Split Crest without any graft.
The biggest problem in agenesis cases is the thickness of the bone, which is bucco-palatal and associated with the need for proper functional and esthetic rehabilitation.
In the case that we reported, because of the lack of thickness, we performed a minimally invasive split crest, which allowed us to correct the implant insertion with immediate loading by a temporary crown, just for aesthetic reasons.
Anatomically guided full arch implant-prosthetic rehabilitations
Authors: Rastelli Sofia , Caporro Giulia , Severino Marco , Bernardi Sara , Botticelli Gianluca , Gasparri Alberto , Pizzolante Tommaso
Published: January 2025

Aim: The loss of dental elements causes anatomical, functional, aesthetic, and psychological changes that reduce the patient’s quality of life and comfort. This study aims to show how implant-prosthetic restorations with full-arch immediate loading can be appropriately performed by an anatomically guided method.
Materials and methods: Two case reports of two male patients aged 69 and 60 years old who needed an implant-prosthetic full arch rehabilitation were documented to describe the surgical treatment.
Discussion: Human anatomy is the basis of any surgical treatment, so in oral surgery, the operator must have a detailed knowledge of the loco-regional anatomy of the head-neck district. For this reason, it is essential to know the topographical limits of the noble structures typically found in daily clinical practice to perform a precise surgical act under safe conditions. The validity of a complete dental arch rehabilitation method is emphasized in literature by inserting two axial implants positioned orthogonally to the occlusal plane in the anterior region and two distal implants inclined with a maximum of 30° distally in the posterior region. The literature reports of inclined implants have been proposed as an alternative to traditional protocols in rehabilitating edentulous jaws. All this can be achieved by carefully studying the clinical case at the beginning of the clinical and radiographic examination. In addition, knowledge of the anatomical reference points must be taken as a reference to obtain a method that is reproducible and adaptable to more clinical cases depending on bone mineralization.
Conclusion: Although it may seem excessively invasive, this method allows us to obtain optimal and reproducible results. It allows us to exploit not only the biomechanical advantages obtained by the structure of the prosthetic polygon but above all, those biological ones based on knowledge of the physiology of bone remodeling.
Bioecology of mites associated with cocoa trees (Theobroma cacao: Malvaceae) in the Bragantina microregion of the state of Pará, Eastern Amazon, Brazil
Authors: Antônia Da Silva Brito Edna , Wurlitzer Wesley Borges , De Castro Iury Silva , Vinhas Naiara Antonia Nunes , Da Silva Noronha Aloyséia Cristina , Ferla Noeli Juarez
Published: January 2025

This study aimed to understand the diversity of associated mites in cocoa growing areas in the municipalities of Bragança and Augusto Corrêa, state of Pará, as well as their distribution in rainy and dry periods. A total of 1,178 mites were collected and the most abundant families were Phytoseiidae, Tetranychidae, Tuckerellidae, Iolinidae and Cunaxidae, in addition to the unidentified taxa of the suborder Oribatida. The most abundant species were Oligonychus sp. (13.9%), Tuckerella ornata (12.2%), Amblyseius n. sp. 1 (12.1%) and Parapronematus sp. (10.5%). Among the predatory mites, the phytoseiid mites were more abundant and prevalent, with Amblyseius n. sp. 1 and Armascirus amazoniensis standing out. Amblyseius n. sp. 1 showed greater abundance in the rainy season than in the dry season, in both municipalities. Great diversity did note, mainly for predatory mites, while phytophagous mites were more abundant.
Low clinical activity score, ‘progressive’ thyroid eye disease: presentations of 1439 patients from a tertiary centre in Hong Kong
Authors: Lai Kenneth Ka Hei , Aljufairi Fatema Mohamed Ali Abdulla , Sebastian Jake Uy , Lai Kei Hei , Chan Karen Kar Wun , Chin Joyce Kar Yee , Chan Regine Yien Ching , Li Chi Lai , Yip Wilson Wai Kuen , Young Alvin Lerrmann , Tham Clement Chee Yung , Pang Chi Pui , Chong Kelvin Kam Lung
Published: January 2025

Background

To report the presenting clinical, serological and treatment profiles of 1439 patients with thyroid eye disease (TED) from a tertiary centre in Hong Kong.

Study populations

Consecutive patients with TED presented to the Thyroid Eye Clinic (TEC), the Chinese University of Hong Kong between 2014 and 2023.

Methods

Prospective cohort and masked review of medical records and orbital images.

Results

A total of 1439 (70% female, 98% Han Chinese) patients with TED (26% ex/current smoker), presented at 43±5.9 years old, were reviewed. The first TED symptoms to TEC evaluation was 6±3 months. 85% had Graves’ disease and 12% were given radioactive iodine before presentation. 35% of patients had a family history of autoimmune thyroid diseases. Baseline thyroid-stimulating hormone receptor antibody, thyroid-stimulating immunoglobulin and thyroid peroxidase antibody were elevated in 75%, 69% and 57% tested. Euthyroid TED (E-TED) was diagnosed in 6%, associated with asymmetric presentation (p<0.001). The most common signs were exophthalmos (69%), upper eyelid retraction (53%), swelling (36%), conjunctival injection (34%) and lower eyelid retraction (32%). Notably, 547 (38%) presented with moderate-to-severe and 150 (10%) vision-threatening TED, whose clinical activity score (CAS) was just 2.4±1.4 and 2.6±1.5, respectively. Male and TED onset after 40 were associated with higher CAS and NOSPEC (No physical signs or symptoms, Only signs, Soft tissue involvement, Proptosis, Extraocular muscle signs, Corneal involvement, and Sight loss) score (both p<0.05). 471 (33%), 361 (25%), 263 (18%) and 138 (9%) patients received intravenous methylprednisolone, orbital radiotherapy, steroid-sparing immunosuppressants and surgical decompression, respectively.

Conclusions

Around one-third (34%) of our cohort presented with ‘low-CAS, progressive’, moderate-to-severe or vision-threatening TED, while only one-fifth (18%) were clinically active (CAS≥3). Our results showed the limitations and unmet need of the existing ‘high-CAS only’ approach, especially in managing ‘non-inflammatory’ TED, prevalent in non-Caucasian populations.
Long-term follow-up of a randomised clinical trial: standard of care versus pre-emptive transjugular intrahepatic portosystemic shunt (TIPSS) in patients with cirrhosis and oesophageal variceal bleeding
Authors: Dunne Philip D J , Manship Thomas , Sinha Rohit , Stanley Adrian J , Lachlan Neil , Shams Aman , Forrest Ewan H , Hayes Peter
Published: January 2025

Background and aims

Pre-emptive transjugular intrahepatic portosystemic shunt (pTIPSS) within 72 hours following acute oesophageal variceal bleeding has potential survival benefit. However, there is uncertainty whether pTIPSS is advantageous over standard of care (SOC) and long-term outcomes remain unstudied.

Approach

Patients recruited to a Scottish randomised control trial were allocated to SOC or pTIPSS and had follow-up extended to 3 years. The primary outcome was 3-year transplant-free survival on intention to treat and per-protocol analysis.

Results

58 patients were initially recruited, 29 patients per group. Of the 29 in the pTIPSS group, only 23 received TIPSS placement due to logistical reasons. On intention to treat analysis, 3-year transplant-free survival rate in the SOC group was significantly higher than that of the pTIPSS group (55.2% vs 20.1%, p=0.006, HR 2.5, 95% CI 1.3 to 4.87). On per-protocol analysis, 3-year transplant-free survival rate in the SOC group was significantly higher than that of the pTIPSS group (55.2% vs 15.4%, p=0.03, HR 2.93, 95% CI 1.27 to 7.94). There were significantly higher rates of sepsis-related death or sepsis-induced liver failure-related death in the pTIPSS group compared with the SOC group (48.2% vs 3.6%, p<0.001, reciprocal of RR 13.0, 95% CI 2.46 to 75.45). There were no differences in other outcomes associated with portal hypertension on intention to treat analysis.

Conclusion

pTIPSS was associated with significantly reduced rates of transplant-free survival at 3 years compared with SOC. This may be due to higher rates of sepsis. Further large studies are required to validate these findings.
C5 complement inhibition versus FcRn modulation in generalised myasthenia gravis
Authors: Huntemann Niklas , Gerischer Lea , Herdick Meret , Nelke Christopher , Stascheit Frauke , Hoffmann Sarah , Öztürk Menekse , Schroeter Christina B , Lehnerer Sophie , Stein Maike , Schubert Charlotte , Schneider-Gold Christiane , Pfeuffer Steffen , Krämer Heidrun H , Konen Franz Felix , Skripuletz Thomas , Pawlitzki Marc , Glaubitz Stefanie , Zschüntzsch Jana , Scherwietes Valerie , Totzeck Andreas , Hagenacker Tim , Meuth Sven G , Meisel Andreas , Ruck Tobias
Published: January 2025

Background

Myasthenia gravis (MG) is an autoimmune disorder affecting neuromuscular junctions, leading to fluctuating muscle weakness. While many patients respond well to standard immunosuppression, a substantial subgroup faces ongoing disease activity. Emerging treatments such as complement factor C5 inhibition (C5IT) and neonatal Fc receptor (FcRn) antagonism hold promise for these patients. However, the current landscape is hindered by a paucity of comparative data that is crucial for treatment decisions.

Objective

This study aims to compare the effectiveness and safety of C5IT and FcRn antagonists in a real-world setting.

Methods

A retrospective analysis of 153 MG patients from 8 German specialised MG centres receiving either C5IT (26 eculizumab, 80 ravulizumab) or efgartigimod (47 patients) was conducted. Propensity score matching (PSM) was employed to compare changes in MG-specific outcome parameters within the first 6 months after treatment initiation, along with safety profiles and concomitant MG therapy.

Results

Both treatment strategies led to rapid clinical improvements and substantial reductions in prednisolone doses. However, insufficient response was noted in 20%–49.1% of patients based on Quantitative MG and MG Activities of Daily Living (MG-ADL) scores. We did not identify any new safety concerns. After PSM, 40 patients remained in each group. In both cohorts, reductions in MG-ADL as prespecified primary study endpoint were comparable. Moreover, analyses of secondary outcome parameters demonstrated similar results for C5IT versus FcRn.

Conclusion

In contrast to current meta-analyses and indirect comparisons of clinical trial data, our real-world study demonstrates comparable efficacy and safety of C5IT and FcRn antagonism in MG.
Research on Personalized Design and Recommendation Systems for Cultural and Creative Products Based on User Behavior Data
Authors: Tang Jinsong , Zhang Yang
Published: January 2025

Personalized recommendation technology involves the process of soliciting user data and prescribing a user interest model and active recommendations for some users. Such kinds of creative products involve subjective judgments and intricate patterns in the aesthetics, and it is always difficult to encode algorithms that are capable of understanding and recommending these subjective aspects. In this paper, the beetle swarm optimization algorithm is incorporated into a refined deep neural network model called the beetle swarm-drive refined deep neural network (BS-RDNN) for the analysis of personalized design and recommendation systems for user behaviors. Information about user behavior and feedback was collected as part of this study. The data were preprocessed using Min-Max normalization. t-distributed stochastic neighbor embedding (t-SNE) is employed to reduce the dataset dimensions. The proposed method is discussed with other types of recommendation algorithms. The proposed method is implemented with the aid of Python software. This result proves that the BS-RDNN method has better performance in terms of precision (91.34%), accuracy (93.24%), F1-score (92.23%), recall (92.44%), AUC (91.42%), and overall satisfaction of the users. Therefore, the use of the suggested system to coordinate with the design ideas of different individuals can benefit the field of cultural and creative products.
Psychological Analysis and Career Decision-Making of College Students Based on Cognitive Algorithms on Distributed Platforms
Authors: Zhang Hai-Yan
Published: January 2025

This research uses cognitive analytics on a distributed computer system to investigate how students’ decision-making behaviors relate to their personality traits. The goal of the research is to improve our knowledge of the variables affecting students’ professional choices and to offer a solid, expandable framework for individualized career counseling. The investigation examines a range of psychological characteristics, such as personality characteristics, cognitive capacities, passions, and socioeconomic status, by utilizing cognitive algorithms. Massive databases may be processed more easily thanks to the distributed system, which also ensures effective computing and analyses in real time. The approach entails gathering information from a heterogeneous student body, then analyzing it and extracting features to find meaningful trends. Neural networks and combination techniques are examples of sophisticated algorithms for machine learning that are used to create models of prediction that help with career suggestions. The results show how well cognitive computers capture the intricate interactions between variables that influence career choices. The flexibility of the distributed platform guarantees the system’s suitability for big colleges and universities, offering students individualized career counseling on a broad scale. This study offers a fresh method for comprehending and assisting students in their professional choices, which makes a significant contribution to the fields of data mining for education and career planning.
Synthesis of gold nanoparticles by flame reduction method
Authors: Inamdar Akbar K. , Inamdar Shamshad A. , Abdullah Mahmood M. S. , Shinde Bapusaheb H. , Chaudhari Sushilkumar , Shelke Satish B. , Inamdar Shaukatali N.
Published: January 2025

The gold nanoparticles (Au NPs) are always recognized as vital and important for human life from the ancient era. But it is very difficult to synthesize the Au NPs in the oxidizing atmosphere for the intended purpose. The innovative flame reduction method utilizing the N, N-Dimethylformamide (DMF) as a solvent was successfully used for the synthesis of precious metal Au NPs. The synthesized Au NPs are bare, so they can be directly used without any further processing as catalyst materials. The Au NPs were synthesized by burning their salt in DMF as solvent and fuel using a simple lamp. The prepared nanoparticles were found readily dispersible in water and DMF giving stable sols. In this paper, we report the synthesis of bare metal nanoparticles using the flame reduction method for the first time. The prepared NPs were collected on the glass surface and used for routine characterization techniques viz., UV-Visible, XRD, HR-TEM and electron diffraction. The Au NPs were 15[Formula: see text]nm in size and showed characteristic absorption peak at 550[Formula: see text]nm. The characteristic peaks observed in XRD of synthesized nanoparticles demonstrated the cubic phase of Au NPs. The formation mechanism of the prepared nanoparticles in open-air flame synthesis has been discussed in detail in this paper.
LLM-Based Multi-Agent Systems for Software Engineering: Literature Review, Vision and the Road Ahead
Authors: He Junda , Treude Christoph , Lo David
Published: January 2025

Integrating Large Language Models (LLMs) into autonomous agents marks a significant shift in the research landscape by offering cognitive abilities that are competitive with human planning and reasoning. This paper explores the transformative potential of integrating Large Language Models into Multi-Agent (LMA) systems for addressing complex challenges in software engineering (SE). By leveraging the collaborative and specialized abilities of multiple agents, LMA systems enable autonomous problem-solving, improve robustness, and provide scalable solutions for managing the complexity of real-world software projects. In this paper, we conduct a systematic review of recent primary studies to map the current landscape of LMA applications across various stages of the software development lifecycle (SDLC). To illustrate current capabilities and limitations, we perform two case studies to demonstrate the effectiveness of state-of-the-art LMA frameworks. Additionally, we identify critical research gaps and propose a comprehensive research agenda focused on enhancing individual agent capabilities and optimizing agent synergy. Our work outlines a forward-looking vision for developing fully autonomous, scalable, and trustworthy LMA systems, laying the foundation for the evolution of Software Engineering 2.0.
Stochastic Matching Networks: Theory and Applications to Matching Platforms
Authors: Mahavir Varma Sushil
Published: January 2025

The past decade has witnessed an accelerated growth of online marketplaces and the incorporation of electric vehicles (EVs) in the fleet of transportation systems. Online marketplaces are online platforms that facilitate transactions between buyers and sellers. These platforms are burgeoning with online gig workers constituting 5-10% of the global workforce. 1 Furthermore, the EV market is also flourishing, with EVs accounting for 14% of new car sales worldwide in 2022, a significant increase from 5% in 2020 2 owing to technological advancements, government backing, and climate change awareness. Such a rise in EV adoption is accompanied by the emergence of EV-based transportation systems constituting a fully electric fleet of taxis.
User-Plane Algorithms for Stateless and Stateful Inference in Programmable Networks
Authors: Akem Aristide Tanyi-Jong
Published: January 2025

In the last decade, the complexity of networks has increased significantly to accommodate the rise of innovative applications. This growing complexity has rendered traditional human-in-the-loop network management approaches inadequate, necessitating greater automation and flexibility in managing these networks. The introduction of Software- Defined Networking (SDN) with a programmable control plane marked a major advancement in this direction, enabling a wide range of network automation applications to be executed within the SDN control plane.
Models for Throughput Maximisation in Distributed Systems
Authors: Olliaro Diletta
Published: January 2025

In today's rapidly advancing computing and telecommunications landscape, analysing the performance of distributed systems is more critical than ever. As systems grow in complexity, the demand for robust analytical tools to evaluate efficiency and performance is always more pressing. Among these tools, queueing theory remains a foundational and versatile approach for system analysis.
Supplementary Table S7 from Cancer Prevalence across Vertebrates
Authors: Compton Zachary T. , Mellon Walker , Harris Valerie K. , Rupp Shawn , Mallo Diego , Kapsetaki Stefania E. , Wilmot Mallory , Kennington Ryan , Noble Kathleen , Baciu Cristina , Ramirez Lucia N. , Peraza Ashley , Martins Brian , Sudhakar Sushil , Aksoy Selin , Furukawa Gabriela , Vincze Orsolya , Giraudeau Mathieu , Duke Elizabeth G. , Spiro Simon , Flach Edmund , Davidson Hannah , Li Christopher I. , Zehnder Ashley , Graham Trevor A. , Troan Brigid V. , Harrison Tara M. , Tollis Marc , Schiffman Joshua D. , Aktipis C. Athena , Abegglen Lisa M. , Maley Carlo C. , Boddy Amy M.
Published: January 2025

Results from the bootstrap analysis of different sample size cutoffs
Supplementary Table S2 from Cancer Prevalence across Vertebrates
Authors: Compton Zachary T. , Mellon Walker , Harris Valerie K. , Rupp Shawn , Mallo Diego , Kapsetaki Stefania E. , Wilmot Mallory , Kennington Ryan , Noble Kathleen , Baciu Cristina , Ramirez Lucia N. , Peraza Ashley , Martins Brian , Sudhakar Sushil , Aksoy Selin , Furukawa Gabriela , Vincze Orsolya , Giraudeau Mathieu , Duke Elizabeth G. , Spiro Simon , Flach Edmund , Davidson Hannah , Li Christopher I. , Zehnder Ashley , Graham Trevor A. , Troan Brigid V. , Harrison Tara M. , Tollis Marc , Schiffman Joshua D. , Aktipis C. Athena , Abegglen Lisa M. , Maley Carlo C. , Boddy Amy M.
Published: January 2025

All data prevalence leaderboard
Supplementary Table S2 from AMG 193, a Clinical Stage MTA-Cooperative PRMT5 Inhibitor, Drives Antitumor Activity Preclinically and in Patients with MTAP-Deleted Cancers
Authors: Belmontes Brian , Slemmons Katherine K. , Su Chun , Liu Siyuan , Policheni Antonia N. , Moriguchi Jodi , Tan Hong , Xie Fang , Aiello Daniel Andrew , Yang Yajing , Lazaro Raul , Aeffner Famke , Rees Matthew G. , Ronan Melissa M. , Roth Jennifer A. , Vestergaard Mikkel , Cowland Sanne , Andersson Jan , Sarvary Ian , Chen Qing , Sharma Pooja , Lopez Patricia , Tamayo Nuria , Pettus Liping H. , Ghimire-Rijal Sudipa , Mukund Susmith , Allen Jennifer R. , DeVoss Jason , Coxon Angela , Rodon Jordi , Ghiringhelli François , Penel Nicolas , Prenen Hans , Glad Sanne , Chuang Chen-Hua , Keyvanjah Kiana , Townsley Danielle M. , Butler John R. , Bourbeau Matthew P. , Caenepeel Sean , Hughes Paul E.
Published: January 2025

Supplementary Table S2: Co-crystal structure data processing and refinement statistics
Supplementary Figure S71 from Cancer Prevalence across Vertebrates
Authors: Compton Zachary T. , Mellon Walker , Harris Valerie K. , Rupp Shawn , Mallo Diego , Kapsetaki Stefania E. , Wilmot Mallory , Kennington Ryan , Noble Kathleen , Baciu Cristina , Ramirez Lucia N. , Peraza Ashley , Martins Brian , Sudhakar Sushil , Aksoy Selin , Furukawa Gabriela , Vincze Orsolya , Giraudeau Mathieu , Duke Elizabeth G. , Spiro Simon , Flach Edmund , Davidson Hannah , Li Christopher I. , Zehnder Ashley , Graham Trevor A. , Troan Brigid V. , Harrison Tara M. , Tollis Marc , Schiffman Joshua D. , Aktipis C. Athena , Abegglen Lisa M. , Maley Carlo C. , Boddy Amy M.
Published: January 2025

Analysis our of our regression results with various data transformation techniques
Supplementary Figure S70 from Cancer Prevalence across Vertebrates
Authors: Compton Zachary T. , Mellon Walker , Harris Valerie K. , Rupp Shawn , Mallo Diego , Kapsetaki Stefania E. , Wilmot Mallory , Kennington Ryan , Noble Kathleen , Baciu Cristina , Ramirez Lucia N. , Peraza Ashley , Martins Brian , Sudhakar Sushil , Aksoy Selin , Furukawa Gabriela , Vincze Orsolya , Giraudeau Mathieu , Duke Elizabeth G. , Spiro Simon , Flach Edmund , Davidson Hannah , Li Christopher I. , Zehnder Ashley , Graham Trevor A. , Troan Brigid V. , Harrison Tara M. , Tollis Marc , Schiffman Joshua D. , Aktipis C. Athena , Abegglen Lisa M. , Maley Carlo C. , Boddy Amy M.
Published: January 2025

Analysis of our results with the outlier species removed
Supplementary Figure S3 from AMG 193, a Clinical Stage MTA-Cooperative PRMT5 Inhibitor, Drives Antitumor Activity Preclinically and in Patients with MTAP-Deleted Cancers
Authors: Belmontes Brian , Slemmons Katherine K. , Su Chun , Liu Siyuan , Policheni Antonia N. , Moriguchi Jodi , Tan Hong , Xie Fang , Aiello Daniel Andrew , Yang Yajing , Lazaro Raul , Aeffner Famke , Rees Matthew G. , Ronan Melissa M. , Roth Jennifer A. , Vestergaard Mikkel , Cowland Sanne , Andersson Jan , Sarvary Ian , Chen Qing , Sharma Pooja , Lopez Patricia , Tamayo Nuria , Pettus Liping H. , Ghimire-Rijal Sudipa , Mukund Susmith , Allen Jennifer R. , DeVoss Jason , Coxon Angela , Rodon Jordi , Ghiringhelli François , Penel Nicolas , Prenen Hans , Glad Sanne , Chuang Chen-Hua , Keyvanjah Kiana , Townsley Danielle M. , Butler John R. , Bourbeau Matthew P. , Caenepeel Sean , Hughes Paul E.
Published: January 2025

Supplementary Figure S3. HCT116 isogenic pair validation
Supplementary Figure S2 from AMG 193, a Clinical Stage MTA-Cooperative PRMT5 Inhibitor, Drives Antitumor Activity Preclinically and in Patients with MTAP-Deleted Cancers
Authors: Belmontes Brian , Slemmons Katherine K. , Su Chun , Liu Siyuan , Policheni Antonia N. , Moriguchi Jodi , Tan Hong , Xie Fang , Aiello Daniel Andrew , Yang Yajing , Lazaro Raul , Aeffner Famke , Rees Matthew G. , Ronan Melissa M. , Roth Jennifer A. , Vestergaard Mikkel , Cowland Sanne , Andersson Jan , Sarvary Ian , Chen Qing , Sharma Pooja , Lopez Patricia , Tamayo Nuria , Pettus Liping H. , Ghimire-Rijal Sudipa , Mukund Susmith , Allen Jennifer R. , DeVoss Jason , Coxon Angela , Rodon Jordi , Ghiringhelli François , Penel Nicolas , Prenen Hans , Glad Sanne , Chuang Chen-Hua , Keyvanjah Kiana , Townsley Danielle M. , Butler John R. , Bourbeau Matthew P. , Caenepeel Sean , Hughes Paul E.
Published: January 2025

Supplementary Figure S2. SPR AMG 193 competitive chaser experiment
Supplementary Data Table 3 from Zongertinib (BI 1810631), an Irreversible HER2 TKI, Spares EGFR Signaling and Improves Therapeutic Response in Preclinical Models and Patients with HER2-Driven Cancers
Authors: Wilding Birgit , Woelflingseder Lydia , Baum Anke , Chylinski Krzysztof , Vainorius Gintautas , Gibson Neil , Waizenegger Irene C. , Gerlach Daniel , Augsten Martin , Spreitzer Fiona , Shirai Yukina , Ikegami Masachika , Tilandyová Sylvia , Scharn Dirk , Pearson Mark A. , Popow Johannes , Obenauf Anna C. , Yamamoto Noboru , Kondo Shunsuke , Opdam Frans L. , Bruining Annemarie , Kohsaka Shinji , Kraut Norbert , Heymach John V. , Solca Flavio , Neumüller Ralph A.
Published: January 2025

Tempus data analysis results.
Supplementary Tables S1 and S2 from Neuro-Mesenchymal Interaction Mediated by a β2-Adrenergic Nerve Growth Factor Feedforward Loop Promotes Colorectal Cancer Progression
Authors: Kobayashi Hiroki , Iida Tadashi , Ochiai Yosuke , Malagola Ermanno , Zhi Xiaofei , White Ruth A. , Qian Jin , Wu Feijing , Waterbury Quin T. , Tu Ruhong , Zheng Biyun , LaBella Jonathan S. , Zamechek Leah B. , Ogura Atsushi , Woods Susan L. , Worthley Daniel L. , Enomoto Atsushi , Wang Timothy C.
Published: January 2025

Supplementary Table S1: List of differentially expressed genes in RNA-seq from norepinephrine- or vehicle-treated AKP tumor organoids. Supplementary Table S2: Gene set enrichment analyses using RNA-seq data from norepinephrine- or vehicle-treated AKP tumor organoids.
Figure 1 from Chromothripsis-Mediated Small Cell Lung Carcinoma
Authors: Rekhtman Natasha , Tischfield Sam E. , Febres-Aldana Christopher A. , Lee Jake June-Koo , Chang Jason C. , Herzberg Benjamin O. , Selenica Pier , Woo Hyung Jun , Vanderbilt Chad M. , Yang Soo-Ryum , Xu Fei , Bowman Anita S. , da Silva Edaise M. , Noronha Anne Marie , Mandelker Diana L. , Mehine Miika , Mukherjee Semanti , Blanco-Heredia Juan , Orgera John J. , Nanjangud Gouri J. , Baine Marina K. , Aly Rania G. , Sauter Jennifer L. , Travis William D. , Savari Omid , Moreira Andre L. , Falcon Christina J. , Bodd Francis M. , Wilson Christina E. , Sienty Jacklynn V. , Manoj Parvathy , Sridhar Harsha , Wang Lu , Choudhury Noura J. , Offin Michael , Yu Helena A. , Quintanal-Villalonga Alvaro , Berger Michael F. , Ladanyi Marc , Donoghue Mark T.A. , Reis-Filho Jorge S. , Rudin Charles M.
Published: January 2025

Cohort selection and clinicopathologic characteristics. A, Schematic diagram of RB1 and TP53 inactivation (outer doughnut) and smoking status (inner doughnut) in a cohort of 600 consecutive SCLC that underwent tNGS by MSK-IMPACT. RB1 and TP53 status was determined based on integrated genomic and IHC data (see “Results”). B, Tabular and (C) pictorial summary of pathologic sample characteristics and sequencing assays performed for 20 patients with aSCLC. See “Methods” for histopathologic criteria. Ki67 not available. D, Summary of metastatic site distribution based on radiologic–pathologic findings (full data in Supplementary Table S2). E–G, Compilation of radiologic and pathologic findings for three representative patients. E, (A01): 19-year-old patient with brain metastasis showing classic undifferentiated morphology of SCLC: small, crowded/molding cells, numerous mitotic figures (>50/2 mm2), apoptotic bodies, and Ki67 of 90%, whereas thoracic sample shows well-differentiated, nested morphology of a carcinoid tumor lacking mitotic figures or apoptotic bodies, and exhibiting Ki67 of 5%–20%. F, Case A08 and (G) case A09 illustrate classic SCLC morphology in primary lung tumor samples, featuring Ki67 rate of 80% and 90%, respectively. n indicates areas of extensive necrosis–a hallmark feature of SCLC. For extended pathologic illustrations, see Supplementary Figs. S1–S4. Scale bars are 100 μm for Ki67 and 50 μm for H&E except for top in F which is 1 mm. H, Comparison of age, Ki67 proliferation index, and smoking pack-years in aSCLC vs. other SCLC (n = 224 for age and Ki67; n = 200 for pack-year smoking). For patients with multiple samples, Ki67 represents the maximal (max) hot-spot rate among all evaluated samples. H&E, hematoxylin and eosin; FNA, fine needle aspiration; Core bx, core biopsy; py, pack-years; tNGS, targeted next-generation sequencing. (C, D created in part with BioRender.com.)
Table S9 from TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T Cells
Authors: Priego Neibla , de Pablos-Aragoneses Ana , Perea-García María , Pieri Valentina , Hernández-Oliver Carolina , Álvaro-Espinosa Laura , Rojas Andrea , Sánchez Oliva , Steindl Ariane , Caleiras Eduardo , García Fernando , García-Martín Santiago , Graña-Castro Osvaldo , García-Mulero Sandra , Serrano Diego , Velasco-Beltrán Paloma , Jiménez-Lasheras Borja , Egia-Mendikute Leire , Rupp Luise , Stammberger Antonia , Meinhardt Matthias , Chaachou-Charradi Anas , Martínez-Saez Elena , Bertero Luca , Cassoni Paola , Mangherini Luca , Pellerino Alessia , Rudà Roberta , Soffietti Riccardo , Al-Shahrour Fatima , Saftig Paul , Sanz-Pamplona Rebeca , Schmitz Marc , Crocker Stephen J. , Calvo Alfonso , Palazón Asís , Valiente Manuel
Published: January 2025

GSEA_comparision between cluster 4 and cluster 5 of brain metastasis-associated astrocytes in human brain metastasis samples. Gene set enrichment analysis (GSEA) of upregulated and downregulated signatures comparing cluster number 4 and number 5 of human brain metastasis associated-astrocytes. The table contains information corresponding to Figure 1I.
Table S17 from TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T Cells
Authors: Priego Neibla , de Pablos-Aragoneses Ana , Perea-García María , Pieri Valentina , Hernández-Oliver Carolina , Álvaro-Espinosa Laura , Rojas Andrea , Sánchez Oliva , Steindl Ariane , Caleiras Eduardo , García Fernando , García-Martín Santiago , Graña-Castro Osvaldo , García-Mulero Sandra , Serrano Diego , Velasco-Beltrán Paloma , Jiménez-Lasheras Borja , Egia-Mendikute Leire , Rupp Luise , Stammberger Antonia , Meinhardt Matthias , Chaachou-Charradi Anas , Martínez-Saez Elena , Bertero Luca , Cassoni Paola , Mangherini Luca , Pellerino Alessia , Rudà Roberta , Soffietti Riccardo , Al-Shahrour Fatima , Saftig Paul , Sanz-Pamplona Rebeca , Schmitz Marc , Crocker Stephen J. , Calvo Alfonso , Palazón Asís , Valiente Manuel
Published: January 2025

GSEA analysis of proteomic data obtained from CD8+ T cells incubated with astrospheres conditioned medium. NES and statistics of the comparisons between the following experimental conditions: CD8+ lymphocytes were cultured with pSTAT3- astrospheres conditioned medium (CM) (D1) and wt (D2) or cKOGFAP-Timp1 (D2KO) pSTAT3+ astrospheres CM processed for phosphoproteomic analysis. The table contains information corresponding to Supplementary Figure 7B.
Table S16 from TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T Cells
Authors: Priego Neibla , de Pablos-Aragoneses Ana , Perea-García María , Pieri Valentina , Hernández-Oliver Carolina , Álvaro-Espinosa Laura , Rojas Andrea , Sánchez Oliva , Steindl Ariane , Caleiras Eduardo , García Fernando , García-Martín Santiago , Graña-Castro Osvaldo , García-Mulero Sandra , Serrano Diego , Velasco-Beltrán Paloma , Jiménez-Lasheras Borja , Egia-Mendikute Leire , Rupp Luise , Stammberger Antonia , Meinhardt Matthias , Chaachou-Charradi Anas , Martínez-Saez Elena , Bertero Luca , Cassoni Paola , Mangherini Luca , Pellerino Alessia , Rudà Roberta , Soffietti Riccardo , Al-Shahrour Fatima , Saftig Paul , Sanz-Pamplona Rebeca , Schmitz Marc , Crocker Stephen J. , Calvo Alfonso , Palazón Asís , Valiente Manuel
Published: January 2025

Predictive analysis of patient-derived organotypic cultures (PDOC) treated with anti-TIMP1. Clinical information of samples treated in patient-derived organotypic brain cultures (PDOCs) of human brain metastasis with Anti-TIMP1 stratified accorded to their Immune Cluster. The table contains information corresponding to Supplementary Figure 5M-N.
Table S14 from TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T Cells
Authors: Priego Neibla , de Pablos-Aragoneses Ana , Perea-García María , Pieri Valentina , Hernández-Oliver Carolina , Álvaro-Espinosa Laura , Rojas Andrea , Sánchez Oliva , Steindl Ariane , Caleiras Eduardo , García Fernando , García-Martín Santiago , Graña-Castro Osvaldo , García-Mulero Sandra , Serrano Diego , Velasco-Beltrán Paloma , Jiménez-Lasheras Borja , Egia-Mendikute Leire , Rupp Luise , Stammberger Antonia , Meinhardt Matthias , Chaachou-Charradi Anas , Martínez-Saez Elena , Bertero Luca , Cassoni Paola , Mangherini Luca , Pellerino Alessia , Rudà Roberta , Soffietti Riccardo , Al-Shahrour Fatima , Saftig Paul , Sanz-Pamplona Rebeca , Schmitz Marc , Crocker Stephen J. , Calvo Alfonso , Palazón Asís , Valiente Manuel
Published: January 2025

Human brain metastases refractory to immune checkpoint blockade. Clinical information of samples from brain metastasis patients that responded to immune checkpoint blockade systemically but not in the brain. The table contains information corresponding to Figure 3H.
Table S10 from TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T Cells
Authors: Priego Neibla , de Pablos-Aragoneses Ana , Perea-García María , Pieri Valentina , Hernández-Oliver Carolina , Álvaro-Espinosa Laura , Rojas Andrea , Sánchez Oliva , Steindl Ariane , Caleiras Eduardo , García Fernando , García-Martín Santiago , Graña-Castro Osvaldo , García-Mulero Sandra , Serrano Diego , Velasco-Beltrán Paloma , Jiménez-Lasheras Borja , Egia-Mendikute Leire , Rupp Luise , Stammberger Antonia , Meinhardt Matthias , Chaachou-Charradi Anas , Martínez-Saez Elena , Bertero Luca , Cassoni Paola , Mangherini Luca , Pellerino Alessia , Rudà Roberta , Soffietti Riccardo , Al-Shahrour Fatima , Saftig Paul , Sanz-Pamplona Rebeca , Schmitz Marc , Crocker Stephen J. , Calvo Alfonso , Palazón Asís , Valiente Manuel
Published: January 2025

GSEA_comparision between STAT3+ clusters of brain metastasis-associated astrocytes in human brain metastasis samples. Gene set enrichment analysis (GSEA) of upregulated and downregulated signatures comparing each STAT3+ cluster and the other clusters of brain metastasis-associated astrocytes. The table contains information corresponding to Figure 1F-I.
Data from Neuro-Mesenchymal Interaction Mediated by a β2-Adrenergic Nerve Growth Factor Feedforward Loop Promotes Colorectal Cancer Progression
Authors: Kobayashi Hiroki , Iida Tadashi , Ochiai Yosuke , Malagola Ermanno , Zhi Xiaofei , White Ruth A. , Qian Jin , Wu Feijing , Waterbury Quin T. , Tu Ruhong , Zheng Biyun , LaBella Jonathan S. , Zamechek Leah B. , Ogura Atsushi , Woods Susan L. , Worthley Daniel L. , Enomoto Atsushi , Wang Timothy C.
Published: January 2025

Abstract
Cancer-associated fibroblasts (CAF) and nerves, components of the tumor microenvironment, have each been shown to directly promote gastrointestinal cancers. However, it remains unknown whether these cells interact with each other to regulate cancer progression. We found that in colorectal cancer, norepinephrine induces ADRB2 (β2-adrenergic receptor)–dependent nerve growth factor (NGF) secretion from CAFs, which in turn increases intratumor sympathetic innervation and norepinephrine accumulation. Adrenergic stimulation accelerates colorectal cancer growth through ADRA2A/Gi-mediated activation of Yes-associated protein (YAP). NGF from CAFs directly enhances colorectal cancer cell growth via the phosphatidylinositol-3-kinase/AKT pathway. Treatment with a tropomyosin receptor kinase (TRK) inhibitor decreased YAP and AKT activation and colorectal cancer progression in mice. In human colorectal cancer, high NGF expression is associated with mesenchymal-like tumor subtype and poor patient survival. These findings suggest a central role for reciprocal CAF–nerve cross-talk in promoting colorectal cancer progression. Blocking this feedforward loop with a TRK inhibitor may represent a potential therapeutic approach for colorectal cancer.
Significance:
Our work demonstrates that the bidirectional interplay between sympathetic nerves and NGF-expressing CAFs drives colorectal tumorigenesis. This study also offers novel mechanistic insights into catecholamine action in colorectal cancer. Inhibiting the neuro-mesenchymal interaction by TRK blockade could be a potential strategy for treating colorectal cancer.