bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrospun Nanometer to Micrometer Scale Biomimetic Synthetic Membrane Scaffolds in Drug Delivery and Tissue Engineering: A Review
Authors: Pazhanimala Shaleena , Vllasaliu Driton , Raimi-Abraham Bahijja
Published: March 2019

The scaffold technology research utilizes biomimicry to produce efficient scaffolds that mimic the natural cell growth environment including the basement membrane for tissue engineering. Because the natural basement membrane is composed of fibrillar protein networks of nanoscale diameter, the scaffold produced should efficiently mimic the nanoscale topography at a low production cost. Electrospinning is a technique that can achieve that. This review discusses the physical and chemical characteristics of the basement membrane and its significance on cell growth and overall focuses on nanoscale biomimetic synthetic membrane scaffolds primarily generated using electrospinning and their application in drug delivery and tissue engineering.
Nonuniform Bessel-Based Radiation Distributions on A Spherically Curved Boundary for Modeling the Acoustic Field of Focused Ultrasound Transducers
Authors: Gutierrez Mario Ibrahin , Ramos Antonio , Gutierrez Josefina , Vera Arturo , Leija Lorenzo
Published: March 2019

Therapeutic focused ultrasound is a technique that can be used with different intensities depending on the application. For instance, low intensities are required in nonthermal therapies, such as drug delivering, gene therapy, etc.; high intensity ultrasound is used for either thermal therapy or instantaneous tissue destruction, for example, in oncologic therapy with hyperthermia and tumor ablation. When an adequate therapy planning is desired, the acoustic field models of curve radiators should be improved in terms of simplicity and congruence at the prefocal zone. Traditional ideal models using uniform vibration distributions usually do not produce adequate results for clamped unbacked curved radiators. In this paper, it is proposed the use of a Bessel-based nonuniform radiation distribution at the surface of a curved radiator to model the field produced by real focused transducers. This proposal is based on the observed complex vibration of curved transducers modified by Lamb waves, which have a non-negligible effect in the acoustic field. The use of Bessel-based functions to approximate the measured vibration instead of using plain measurements simplifies the rationale and expands the applicability of this modeling approach, for example, when the determination of the effects of ultrasound in tissues is required.
Design and Application of the BiVib Audio-Tactile Piano Sample Library
Authors: Papetti Stefano , Avanzini Federico , Fontana Federico
Published: March 2019

A library of piano samples composed of binaural recordings and keyboard vibrations has been built, with the aim of sharing accurate data that in recent years have successfully advanced the knowledge on several aspects about the musical keyboard and its multimodal feedback to the performer. All samples were recorded using calibrated measurement equipment on two Yamaha Disklavier pianos, one grand and one upright model. This paper documents the sample acquisition procedure, with related calibration data. Then, for sound and vibration analysis, it is shown how physical quantities such as sound intensity and vibration acceleration can be inferred from the recorded samples. Finally, the paper describes how the samples can be used to correctly reproduce binaural sound and keyboard vibrations. The library has potential to support experimental research about the psycho-physical, cognitive and experiential effects caused by the keyboard’s multimodal feedback in musicians and other users, or, outside the laboratory, to enable an immersive personal piano performance.
A Comparative Study of Stack Emissions from Straight-Line and Zigzag Brick Kilns in Nepal
Authors: Nepal Sangeet , Mahapatra Parth Sarathi , Adhikari Sagar , Shrestha Sujan , Sharma Prakash , Shrestha Kundan Lal , Pradhan Bidya Banmali , Puppala Siva Praveen
Published: March 2019

Nepal has approximately 1000 operational brick kilns, which contribute significantly to ambient air pollution. They also account for 1.81% of the total bricks produced in the South Asian region. Little is known about their emissions, which are consequently not represented in regional/global emission inventories. This study compared emissions from seven brick kilns. Four were Fixed Chimney Bull’s Trench Kilns (FCBTKs) and three were Induced-Draught Zigzag Kilns (IDZKs). The concentrations of carbon dioxide (CO2), sulfur dioxide (SO2), black carbon (BC), and particulate matter (PM) with a diameter less than 2.5 µm (PM2.5) were measured. The respective emission factors (EFs) were estimated using the carbon mass balance method. The average fuel-based EF for CO2, SO2, PM2.5, and BC were estimated as 1633 ± 134, 22 ± 22, 3.8 ± 2.6 and 0.6 ± 0.2 g per kg, respectively, for all FCBTKs. Those for IDZKs were 1981 ± 232, 24 ± 22, 3.1 ± 1, and 0.4 ± 0.2 g per kg, respectively. Overall, the study found that converting the technology from straight-line kilns to zigzag kilns can reduce PM2.5 emissions by ~20% and BC emissions by ~30%, based on emission factor estimates of per kilogram of fuel. While considering per kilogram of fired brick, emission reductions were approximately 40% for PM2.5 and 55% for BC, but this definitely depends on proper stacking and firing procedures.
An Observational Study on Cloud Spectral Width in North China
Authors: Wang Yuan , Niu Shengjie , Lu Chunsong , Liu Yangang , Chen Jingyi , Yang Wenxia
Published: March 2019

Cloud droplet size distribution (CDSD) is a critical characteristic for a number of processes related to clouds, considering that cloud droplets are formed in different sizes above the cloud-base. This paper analyzes the in-situ aircraft measurements of CDSDs and aerosol concentration ( N a ) performed in stratiform clouds in Hebei, China, in 2015 to reveal the characteristics of cloud spectral width, commonly known as relative dispersion ( ε , ratio of standard deviation (σ) to mean radius (r) of the CDSD). A new algorithm is developed to calculate the contributions of droplets of different sizes to ε . It is found that small droplets with the size range of 1 to 5.5 μm and medium droplets with the size range of 5.5 to 10 μm are the major contributors to ε, and the medium droplets generally dominate the change of ε. The variation of ε with N a can be well explained by comparing the normalized changes of σ and r ( k σ / σ and k r / r ), rather than k σ and k r only ( k σ is Δσ/Δ N a and k r is Δr/Δ N a ). From the perspective of external factors affecting ε change, the effects of N a and condensation are examined. It is found that ε increases initially and decreases afterward as N a increases, and “condensational broadening” occurs up to 1 km above cloud-base, potentially providing observational evidence for recent numerical simulations in the literature.
Effects of the Particulate Matter Index and Particulate Evaluation Index of the Primary Reference Fuel on Particulate Emissions from Gasoline Direct Injection Vehicles
Authors: Zhao Yaowei , Li Xinghu , Hu Shouxin , Ma Chenfei
Published: March 2019

The purpose of this experimental study was to evaluate the range of particulate mass (PM) and particulate number (PN) results from gasoline direct injection (GDI) vehicles by using four test fuels with a range of particulate matter index (PMI) from 1.38 to 2.39 and particulate evaluation index (PEI) from 0.89 to 1.92. The properties of four test fuels were analyzed with detailed hydrocarbon analysis (DHA). Two passenger cars with a GDI engine were tested with four test fuels by conducting the China 6 test procedure, which is equivalent to the worldwide harmonized light-duty vehicle test procedure (WLTP). When the fuels could meet the China 6 primary reference fuel standard with PMI from 1.38 to 2.04 and PEI from 0.89 to 1.59, the PM variation of Vehicle B was from 1.94 mg/km to 3.32 mg/km and of Vehicle A was from 2.55 mg/km to 4.15 mg/km, respectively. In addition, the PN variation of Vehicle B was from 1.57 × 1012 #/km to 3.38 × 1012 #/km and of Vehicle A was from 3.02 × 1012 #/km to 4.80 × 1012 #/km. It was noted that the two different cars had a unique response and sensitivity by using the different fuels, but PMI and PEI did trend with both the PM and the PN response. All PM and PN results from the two cars had an excellent correlation R2 > 0.94 with PMI and R2 > 0.90 with PEI. Therefore, PMI/PEI would be the appropriate specification for sooting tendency in reference fuel standards of emission regulations.
Comparison of Two Automatic Identification Algorithms for Cyclones Affecting the Changjiang River–Huaihe River Valleys
Authors: Hu Ye , Lu Chuhan , Qin Yujing , Cai Jiaxi
Published: March 2019

In this study, two commonly used automated methods of detecting cyclones in the lower troposphere were compared with respect to various features of cyclone activity. The first method is based on the neighbor cyclone center point (NCP), while the second method is the cyclone area algorithm (CAA), which relies on the detection of the outermost enclosed contour to identify the horizontal structure of a cyclone. We obtained climatologies of cyclones that affected the Changjiang River–Huaihe River Valleys (CHV) of China (derived from ERA-Interim data for 1979–2015) and compared their structures. We found that the distribution of the track and the cyclogenesis locations of influential cyclones (ICs) showed a consistent spatial pattern between the NCP and CAA. However, there were still notable differences between the statistical features of cyclone activity derived by the NCP and CAA: (1) Only <46% of cyclones shared the same cyclone center between these two schemes. (2) ICs derived from the CAA typically had longer lifetimes and travel distances, with stronger central intensities than those from the NCP. (3) The track of ICs by the CAA with high resolution was consistent with that of ICs by the low-resolution CAA as well as the low-resolution NCP. However, compared to other methods, the high-resolution NCP presented large deviations during the early cyclone stage. The involvement of open systems in the NCP resulted in weaker cyclone intensities and increased uncertainty in cyclone tracking. On the other hand, more cyclones with stronger intensities and longer lifetimes coming from the midlatitudes were detected using the CAA. In addition, the short-lifetime ICs (<18 h) found using the CAA were active (39%) in the CHV, and were typically excluded by the NCP. These ICs had comparable center intensity and showed a good correlation with the occurrence of simultaneous rainfall events.
Stochastic Electrodynamics: The Closest Classical Approximation to Quantum Theory
Authors: Boyer Timothy H.
Published: March 2019

Stochastic electrodynamics is the classical electrodynamic theory of interacting point charges which includes random classical radiation with a Lorentz-invariant spectrum whose scale is set by Planck’s constant. Here, we give a cursory overview of the basic ideas of stochastic electrodynamics, of the successes of the theory, and of its connections to quantum theory.
Partial Replacement of Maltodextrin by Sweet Potato Flour (Ipomoea Batatas L. Lamarck) in the Development of a Shake Beverage
Authors: Contini Gisele Kirchbaner , de Oliveira Fabielli Priscila , Martins Alana , Córdova Katielle Rosalva Voncik
Published: March 2019

Sweet potato flour contains low-glycemic complex carbohydrates and, when it is ingested, prevents insulin spikes and prolongs the feeling of satiety. The aim of this study was to elaborate and to verify the acceptability of the shake with the total or partial substitution of maltodextrin for sweet potato flour. To elaborate the shake beverage, we used a 22 factorial design, with three central points, thus generating seven formulations. For the taste, color, texture, appearance, acceptance and attitude of purchase properties, sensory tests were conducted using a nine-point hedonic scale and panelists (n = 50). The highest acceptability formulations, formulations 3 (10% sweet potato flour; 25% maltodextrin) and 7 (0% sweet potato flour; 25% maltodextrin), were submitted to pH, moisture, ash, protein, lipid, crude fiber and total carbohydrate analyses. The statistical difference between the formulations from the T test (p < 0.05) was verified for the moisture, ash and lipid parameters. Formulation 3 presented higher values of moisture (93.26 ± 0.57) and lipids (1.91 ± 0.01), and formulation 7 had higher values of ash (0.39 ± 0.01). The results of the sensorial and physicochemical analyses of the shake indicate that sweet potato flour shows potential for the elaboration of this drink.
An Overview on Biogenic Amines in Wine
Authors: Costantini Antonella , Vaudano Enrico , Pulcini Laura , Carafa Tommaso , Garcia-Moruno Emilia
Published: March 2019

Biogenic amines (BAs) are low molecular weight compounds formed from precursor amino acids, mainly by microbial decarboxylation. The presence of these compounds is important in the food and beverage industry because, in high amounts, they can lead to negative effects on consumers. In this review, we illustrate the critical aspects needed to control the formation of BAs during winemaking and their presence in the final product. Recent biotechnological approaches related to microorganisms and their ability to reduce BAs are illustrated. The current methods used for BA detection and quantification are also presented. These methods are very important to consider, as BAs can serve as markers for the quality assessment of products. The information presented here offers an overview useful for identifying specific parameters and conditions which should be controlled to minimise BA content in wine; knowledge about BAs in foods and beverages has been accumulating in recent years, not only to ensure and improve quality (since BAs have been used as an indicator of spoilage) but especially to guarantee consumer safety due to the potential toxic effects of BAs on humans.
Biocatalyst Potential of Cellulose-Degrading Microorganisms Isolated from Orange Juice Processing Waste
Authors: Zerva Ioanna , Remmas Nikolaos , Ntougias Spyridon
Published: March 2019

Cellulases can be applied as macerating and peeling enzymes in the orange juice processing industry. In this work, indigenous cellulose-degrading microorganisms were isolated from orange juice processing waste through successive enrichment procedures using carboxymethyl cellulose (CMC) as the sole carbon source. A total of 24 microbial isolates were screened for their ability to grow in CMC liquid medium, resulting in the selection of seven isolates. The latter were further assessed by determining their endo-1,4-β-d-glucanase, exo-1,4-β-d-glucanase, and β-1,4-d-glucosidase activities, of which their respective activities were as high as 3.89, 10.67, and 10.69 U/mg protein. All cellulose-degraders selected belonged to the genus Paenibacillus, although to distinct operational taxonomic units related to P. xylanexedens, P. tundrae, and P. pabuli (operational taxonomic unit—OTU#1) and to P. wynnii, P. odorifer, and P. donghaensis (OTU#2) spectrum. Regarding the cellulase activities of the orange juice processing waste, endo-1,4-β-d-glucanase activity (4.00 ± 0.11 U/g) was exerted only extracellularly, whereas exo-1,4-β-d-glucanase (2.60 ± 0.19 U/g) and β-1,4-d-glucosidase (5.69 ± 0.23 U/g) activities were exhibited both extracellularly and intracellularly. In conclusion, orange juice processing waste can be considered as a valuable source for the isolation of cellulose-degrading microbiota with potential uses in beverage industry, solid state fermentation and energy production.
Conserved Glycines Control Disorder and Function in the Cold-Regulated Protein, COR15A
Authors: Sowemimo Oluwakemi , Knox-Brown Patrick , Borcherds Wade , Rindfleisch Tobias , Thalhammer Anja , Daughdrill Gary
Published: March 2019

Cold-regulated (COR) 15A is an intrinsically disordered protein (IDP) from Arabidopsis thaliana important for freezing tolerance. During freezing-induced cellular dehydration, COR15A transitions from a disordered to mostly α-helical structure. We tested whether mutations that increase the helicity of COR15A also increase its protective function. Conserved glycine residues were identified and mutated to alanine. Nuclear magnetic resonance (NMR) spectroscopy was used to identify residue-specific changes in helicity for wildtype (WT) COR15A and the mutants. Circular dichroism (CD) spectroscopy was used to monitor the coil–helix transition in response to increasing concentrations of trifluoroethanol (TFE) and ethylene glycol. The impact of the COR15A mutants on the stability of model membranes during a freeze–thaw cycle was investigated by fluorescence spectroscopy. The results of these experiments showed the mutants had a higher content of α-helical structure and the increased α-helicity improved membrane stabilization during freezing. Comparison of the TFE- and ethylene glycol-induced coil–helix transitions support our conclusion that increasing the transient helicity of COR15A in aqueous solution increases its ability to stabilize membranes during freezing. Altogether, our results suggest the conserved glycine residues are important for maintaining the disordered structure of COR15A but are also compatible with the formation of α-helical structure during freezing induced dehydration.
Assessing Anxiety Disorders Using Wearable Devices: Challenges and Future Directions
Authors: Elgendi Mohamed , Menon Carlo
Published: March 2019

Wearable devices (WD) are starting to increasingly be used for interventions to promote well-being by reducing anxiety disorders (AD). Electrocardiogram (ECG) signal is one of the most commonly used biosignals for assessing the cardiovascular system as it significantly reflects the activity of the autonomic nervous system during emotional changes. Little is known about the accuracy of using ECG features for detecting ADs. Moreover, during our literature review, a limited number of studies were found that involve ECG collection using WD for promoting mental well-being. Thus, for the sake of validating the reliability of ECG features for detecting anxiety in WD, we screened 1040 articles, and only 22 were considered for our study; specifically 6 on panic, 4 on post-traumatic stress, 4 on generalized anxiety, 3 on social, 3 on mixed, and 2 on obsessive-compulsive anxiety disorder articles. Most experimental studies had controversial results. Upon reviewing each of these papers, it became apparent that the use of ECG features for detecting different types of anxiety is controversial, and the use of ECG-WD is an emerging area of research, with limited evidence suggesting its reliability. Due to the clinical nature of most studies, it is difficult to determine the specific impact of ECG features on detecting ADs, suggesting the need for more robust studies following our proposed recommendations.
Repeat Instability in the Fragile X-Related Disorders: Lessons from a Mouse Model
Authors: Zhao Xiaonan , Gazy Inbal , Hayward Bruce , Pintado Elizabeth , Hwang Ye Hyun , Tassone Flora , Usdin Karen
Published: March 2019

The fragile X-related disorders (FXDs) are a group of clinical conditions that result primarily from an unusual mutation, the expansion of a CGG-repeat tract in exon 1 of the FMR1 gene. Mouse models are proving useful for understanding many aspects of disease pathology in these disorders. There is also reason to think that such models may be useful for understanding the molecular basis of the unusual mutation responsible for these disorders. This review will discuss what has been learnt to date about mechanisms of repeat instability from a knock-in FXD mouse model and what the implications of these findings may be for humans carrying expansion-prone FMR1 alleles.
Implication of Voltage-Gated Potassium Channels in Neoplastic Cell Proliferation
Authors: Serrano-Novillo Clara , Capera Jesusa , Colomer-Molera Magalí , Condom Enric , Ferreres Joan Carles , Felipe Antonio
Published: March 2019

Voltage-gated potassium channels (Kv) are the largest group of ion channels. Kv are involved in controlling the resting potential and action potential duration in the heart and brain. Additionally, these proteins participate in cell cycle progression as well as in several other important features in mammalian cell physiology, such as activation, differentiation, apoptosis, and cell volume control. Therefore, Kv remarkably participate in the cell function by balancing responses. The implication of Kv in physiological and pathophysiological cell growth is the subject of study, as Kv are proposed as therapeutic targets for tumor regression. Though it is widely accepted that Kv channels control proliferation by allowing cell cycle progression, their role is controversial. Kv expression is altered in many cancers, and their participation, as well as their use as tumor markers, is worthy of effort. There is an ever-growing list of Kv that remodel during tumorigenesis. This review focuses on the actual knowledge of Kv channel expression and their relationship with neoplastic proliferation. In this work, we provide an update of what is currently known about these proteins, thereby paving the way for a more precise understanding of the participation of Kv during cancer development.
Open Partial Horizontal Laryngectomies for T3–T4 Laryngeal Cancer: Prognostic Impact of Anterior vs. Posterior Laryngeal Compartmentalization
Authors: Del Bon Francesca , Piazza Cesare , Lancini Davide , Paderno Alberto , Bosio Paolo , Taboni Stefano , Morello Riccardo , Montalto Nausica , Missale Francesco , Incandela Fabiola , Marchi Filippo , Filauro Marta , Deganello Alberto , Peretti Giorgio , Nicolai Piero
Published: March 2019

Open partial horizontal laryngectomies (OPHLs) are well-established and oncologically safe procedures for intermediate–advanced laryngeal cancers (LC). T–N categories are well-known prognosticators: herein we tested if “anterior” vs. “posterior” tumor location (as defined in respect to the paraglottic space divided according to a plane passing through the arytenoid vocal process, perpendicular to the ipsilateral thyroid lamina) may represent an additional prognostic factor. We analyzed a retrospective cohort of 85 T3–4a glottic LCs, treated by Type II or III OPHL (according to the European Laryngological Society classification) from 2005 to 2017 at two academic institutions. Five-year overall survival (OS), disease-specific survivals (DSS), and recurrence-free survivals (RFS) were compared according to tumor location and pT category. Anterior and posterior tumors were 43.5% and 56.5%, respectively, 78.8% of lesions were T3 and 21.2% were T4a. Five-year OS, DSS, and RFS for T3 were 74.1%, 80.5%, and 63.4%, respectively, and for T4a 71.8%, 71.8%, and 43%, respectively (p not significant). In relation to tumor location, the survival outcomes were 91%, 94.1%, and 72.6%, respectively, for anterior tumors, and 60.3%, 66.3%, and 49.1%, respectively, for posterior lesions (statistically significant differences). These data provide evidence that laryngeal compartmentalization is a valid prognosticator, even more powerful than the pT category.
Role of Notch Signaling Pathway in Glioblastoma Pathogenesis
Authors: Bazzoni Riccardo , Bentivegna Angela
Published: March 2019

Notch signaling is an evolutionarily conserved pathway that regulates important biological processes, such as cell proliferation, apoptosis, migration, self-renewal, and differentiation. In mammals, Notch signaling is composed of four receptors (Notch1–4) and five ligands (Dll1-3–4, Jagged1–2) that mainly contribute to the development and maintenance of the central nervous system (CNS). Neural stem cells (NSCs) are the starting point for neurogenesis and other neurological functions, representing an essential aspect for the homeostasis of the CNS. Therefore, genetic and functional alterations to NSCs can lead to the development of brain tumors, including glioblastoma. Glioblastoma remains an incurable disease, and the reason for the failure of current therapies and tumor relapse is the presence of a small subpopulation of tumor cells known as glioma stem cells (GSCs), characterized by their stem cell-like properties and aggressive phenotype. Growing evidence reveals that Notch signaling is highly active in GSCs, where it suppresses differentiation and maintains stem-like properties, contributing to Glioblastoma tumorigenesis and conventional-treatment resistance. In this review, we try to give a comprehensive view of the contribution of Notch signaling to Glioblastoma and its possible implication as a target for new therapeutic approaches.
Pre-Operative Versus Post-Operative Radiosurgery of Brain Metastases—Volumetric and Dosimetric Impact of Treatment Sequence and Margin Concept
Authors: El Shafie Rami A. , Tonndorf-Martini Eric , Schmitt Daniela , Weber Dorothea , Celik Aylin , Dresel Thorsten , Bernhardt Denise , Lang Kristin , Hoegen Philipp , Adeberg Sebastian , Paul Angela , Debus Jürgen , Rieken Stefan
Published: March 2019

Background: Pre-operative radiosurgery (SRS) preceding the resection of brain metastases promises to circumvent limitations of post-operative cavity SRS. It minimizes uncertainties regarding delineation and safety margins and could reduce dose exposure of the healthy brain (HB). Methods: We performed a systematic treatment plan comparison on 24 patients who received post-operative radiosurgery of the resection cavity at our institution. Comparative treatment plans were calculated for hypofractionated stereotactic radiotherapy (7 × 5 Gray (Gy)) in a hypothetical pre-operative (pre-op) and two post-operative scenarios, either with (extended field, post-op-E) or without the surgical tract (involved field, post-op-I). Detailed volumetric comparison of the resulting target volumes was performed, as well as dosimetric comparison focusing on targets and the HB. Results: The resection cavity was significantly smaller and different in morphology from the pre-operative lesion, yielding a low Dice Similarity Coefficient (DSC) of 53% (p = 0.019). Post-op-I and post-op-E targets showed high similarity (DSC = 93%), and including the surgical tract moderately enlarged resulting median target size (18.58 ccm vs. 22.89 ccm, p < 0.001). Dosimetric analysis favored the pre-operative treatment setting since it significantly decreased relevant dose exposure of the HB (Median volume receiving 28 Gy: 6.79 vs. 10.79 for pre-op vs. post-op-E, p < 0.001). Dosimetrically, pre-operative SRS is a promising alternative to post-operative cavity irradiation that could furthermore offer practical benefits regarding delineation and treatment planning. Comparative trials are required to evaluate potential clinical advantages of this approach.
Usefulness and Limitations of Comprehensive Characterization of mRNA Splicing Profiles in the Definition of the Clinical Relevance of BRCA1/2 Variants of Uncertain Significance
Authors: Gelli Elisa , Colombo Mara , Pinto Anna Maria , De Vecchi Giovanna , Foglia Claudia , Amitrano Sara , Morbidoni Valeria , Imperatore Valentina , Manoukian Siranoush , Baldassarri Margherita , Lo Rizzo Caterina , Catania Lorenza , Frullanti Elisa , Tagliafico Enrico , Cortesi Laura , Spaggiari Federica , Mencarelli Maria Antonietta , Trevisson Eva , Radice Paolo , Renieri Alessandra , Ariani Francesca
Published: March 2019

Highly penetrant variants of BRCA1/2 genes are involved in hereditary predisposition to breast and ovarian cancer. The detection of pathogenic BRCA variants has a considerable clinical impact, allowing appropriate cancer-risk management. However, a major drawback is represented by the identification of variants of uncertain significance (VUS). Many VUS potentially affect mRNA splicing, making transcript analysis an essential step for the definition of their pathogenicity. Here, we characterize the impact on splicing of ten BRCA1/2 variants. Aberrant splicing patterns were demonstrated for eight variants whose alternative transcripts were fully characterized. Different events were observed, including exon skipping, intron retention, and usage of de novo and cryptic splice sites. Transcripts with premature stop codons or in-frame loss of functionally important residues were generated. Partial/complete splicing effect and quantitative contribution of different isoforms were assessed, leading to variant classification according to Evidence-based Network for the Interpretation of Mutant Alleles (ENIGMA) consortium guidelines. Two variants could be classified as pathogenic and two as likely benign, while due to a partial splicing effect, six variants remained of uncertain significance. The association with an undefined tumor risk justifies caution in recommending aggressive risk-reduction treatments, but prevents the possibility of receiving personalized therapies with potential beneficial effect. This indicates the need for applying additional approaches for the analysis of variants resistant to classification by gene transcript analyses.
PD-1 Inhibitors-Related Neurological Toxicities in Patients with Non-Small-Cell Lung Cancer: A Literature Review
Authors: Mirabile Aurora , Brioschi Elena , Ducceschi Monika , Piva Sheila , Lazzari Chiara , Bulotta Alessandra , Viganò Maria Grazia , Petrella Giovanna , Gianni Luca , Gregorc Vanesa
Published: March 2019

The advent of immune checkpoint inhibitors gave rise to a new era in oncology and general medicine. The increasing use of programmed death-1 (PD-1) inhibitors in non-small cell lung cancer and in other malignancies means clinicians have to face up to new challenges in managing immune-related adverse events (irAEs), which often resemble autoimmune diseases. Neurological irAEs represent an emerging toxicity related to immunotherapy, and it is mandatory to know how to monitor, recognize, and manage them, since they can rapidly lead to patient death if untreated. Guidelines for the diagnosis and treatment of these irAEs have been recently published but sharing some of the most unusual clinical cases is crucial, in our opinion, to improve awareness and to optimize the approach for these patients. A literature review on the diagnosis and treatment of immune-related neurotoxicity’s has been conducted starting from the report of four cases of neurological irAEs regarding cases of polyneuropathy, myasthenia gravis, Bell’s palsy, and encephalopathy, all of which occurred in oncological patients receiving PD-1 inhibitors (pembrolizumab and nivolumab) for the treatment of non-oncogene addicted advanced non-small cell lung cancer. The exclusion of other differential diagnoses and the correlation between the suspension of immunotherapy and improvement of symptoms suggest that immunotherapy could be the cause of the neurological disorders reported.
Reactivity and Heavy Metal Removal Capacity of Calcium Alginate Beads Loaded with Ca–Al Layered Double Hydroxides
Authors: Borgiallo Andres , Rojas Ricardo
Published: March 2019

Layered double hydroxides (LDHs) present multiple applications due to their versatility and reactivity. Thus, Ca–Al LDHs with Friedel’s salt structure (HC) have been proposed as heavy metal scavengers due to their buffering capacity at basic pHs. Nevertheless, the control of the reactivity of LDHs such as HC is necessary to optimize their applications. Here, the reactivity of an HC prepared by a coprecipitation method was modified by its inclusion in calcium alginate (CaAlg) beads prepared by ionic gelation. The obtained beads (CaAlg/HC) showed good dispersion of the HC particles in the alginate matrix and were used to test the acid base reactivity and heavy metal uptake capacity compared with pure CaAlg beads and HC powder separately. The pH buffering capacity of CaAlg beads was enriched by the inclusion of HC that, in turn, was modulated in its reactivity. Thus, the HC dissolution times changed from mere seconds for the powder to tens of minutes when enclosed in the beads in a kinetic profile determined by the diffusive step. On the other hand, Cu2+ uptake capacity of CaAlg/HC beads combined the Cu(OH)2 precipitation capacity of HC with the complexation capacity of alginate, reaching good affinity and capacity for the obtained beads. Nevertheless, the precipitation of the hydroxide was produced outside the bead, which would induce the addition of an additional separation step to produce an acceptable Cu2+ elimination.
Electrochemical Immuno- and Aptasensors for Mycotoxin Determination
Authors: Evtugyn Gennady , Hianik Tibor
Published: March 2019

Modern analysis of food and feed is mostly focused on development of fast and reliable portable devices intended for field applications. In this review, electrochemical biosensors based on immunological reactions and aptamers are considered in the determination of mycotoxins as one of most common contaminants able to negatively affect human health. The characteristics of biosensors are considered from the point of view of general principles of bioreceptor implementation and signal transduction providing sub-nanomolar detection limits of mycotoxins. Moreover, the modern trends of bioreceptor selection and modification are discussed as well as future trends of biosensor development for mycotoxin determination are considered.
The Indian Ocean Dipole: A Missing Link between El Niño Modokiand Tropical Cyclone Intensity in the North Indian Ocean
Authors: Arora Kopal , Dash Prasanjit
Published: March 2019

This study is set out to understand the impact of El Niño Modoki and the Tropical Cyclone Potential Intensity (TCPI) in the North Indian Ocean. We also hypothesized and tested if the Indian Ocean Dipole (IOD) reveals a likely connection between the two phenomena. An advanced mathematical tool namely the Empirical Mode Decomposition (EMD) is employed for the analysis. A major advantage of using EMD is its adaptability approach to deal with the non-linear and non-stationary signals which are similar to the signals used in this study and are also common in both atmospheric and oceanic sciences. This study has identified IOD as a likely missing link to explain the connection between El Niño Modoki and TCPI. This lays the groundwork for future research into this connection and its possible applications in meteorology.
Unevenness of Thin Liquid Layer by Contact Angle Variation of Substrate during Coating Process
Authors: Kim Na Kyong , Kang Dong Hee , Kang Hyun Wook
Published: March 2019

During a thin film application, the surface of the coating liquid applied to the substrate becomes uneven because of the geometry of the substrate, viscosity of the coating liquid, surface tension, and its contact angle with the substrate. The surface is particularly uneven at the edge corner portion of the substrate and is thicker than the average coating thickness. This study used the volume-of-fluid (VOF) method to examine the surface unevenness of the coating liquid in terms of the contact angle of the substrate surface and sides. After the coating liquid was evenly applied to the substrate, the maximum height of the uneven region of the coating liquid at the edge of the substrate increased as time passed. The point of maximum height moved away from the edge corner portion of the substrate. The coating liquid applied to the substrate with a contact angle less than 90° exhibited a pinning effect in which the contact point was fixed at the edge. The surface unevenness was more pronounced in the absence of the pinning effect than in its presence, due to the effects of the viscosity of the coating fluid and the surface energy of the substrate.
Chitosan/Thyme Oil Systems as Affected by Stabilizing Agent: Physical and Antimicrobial Properties
Authors: Sedlaříková Jana , Janalíková Magda , Rudolf Ondřej , Pavlačková Jana , Egner Pavlína , Peer Petra , Varaďová Vendula , Krejčí Jiří
Published: March 2019

Antimicrobial biopolymer films and coatings are of great interest for many applications. Different chitosan systems were prepared and characterized to evaluate the effect of their composition on the physical and antimicrobial properties. Three types of emulsifiers (Tween 20, 80, and 85) were used as stabilizing agents, combined with thyme essential oil (from two producers) applied as an active substance. A predominant role of the applied stabilizer and its hydrophilic–lipophilic balance value was proven. The incorporation of thyme essential oil and surfactant into the chitosan matrix led to a significant decrease of particle size in film-forming solutions, as well as a thickness increase and the enhancement of the barrier properties in chitosan films. Antimicrobial effects were provided even at the lowest tested concentration of thyme essential oil. Hence, the prepared chitosan films represent promising candidates in antimicrobial packaging applications.
Cyclic Hot Corrosion Failure Behaviors of EB-PVD TBC Systems in the Presence of Sulfate and Vanadate Molten Salts
Authors: Ozgurluk Yasin , Doleker Kadir Mert , Ozkan Dervis , Ahlatci Hayrettin , Karaoglanli Abdullah Cahit
Published: March 2019

The cold gas dynamic spray (CGDS) method has been considered a promising technology to produce a metallic bond coat for thermal barrier coating (TBC) systems. In this study, CoNiCrAlY bond coats produced by CGDS method were coated with yttria-stabilized zirconia (YSZ) by electron beam physical vapor deposition (EB-PVD). TBCs were subjected to 50 wt % V2O5 and 50 wt % Na2SO4 molten hot corrosion salt combinations at 1000 °C. In the case of YSZ top coat on TBCs, the reaction between Na2SO4, V2O5, and Y2O3 salts generates YVO4 crystals, and these structures cause the transformation of tetragonal ZrO2 to monoclinic ZrO2. This situation occurs under operating conditions that lead to TBC failure. Hot corrosion behavior and the related failure mechanisms of TBC systems were investigated and discussed using scanning electron microscope (SEM), energy dispersive spectroscopy (EDS) analysis, and X-ray diffractometer (XRD).
Influence of Silicon-Modified Al Powders (SiO2@Al) on Anti-oxidation Performance of Al2O3-SiO2 Ceramic Coating for Carbon Steel at High Temperature
Authors: Yu Bo , Fu Guoyan , Cui Yanbin , Zhang Xiaomeng , Tu Yubo , Du Yingchao , Zuo Gaohong , Ye Shufeng , Wei Lianqi
Published: March 2019

In this paper, silicon-modified Al powders (SiO2@Al) were prepared by tetraethyl orthosilicate (TEOS) hydrolysis under alkaline conditions. Using SiO2@Al as additives, a new Al2O3-SiO2 ceramic coating (ASMA) was formed on carbon steel to prevent carbon steel from oxidization at 1250 °C for 120 min. Compared with the Al2O3-SiO2 ceramic coating without additive (AS), ASMA showed a remarkably better anti-oxidation performance, especially during the temperature-rise period. According to the characterization conducted by TG-DTA, XRD and SEM-EDS, it was found that the metallic Al in ASMA melted at 660 °C and reacted with SiO2 on its surface, which generated local high temperature and accelerated the sintering of ceramic raw materials. The mullite and hercynite formed in ASMA also played a major role for enhancing the anti-oxidation performance of ceramic coating.
Software Requirement Specification Based on a Gray Box for Embedded Systems: A Case Study of a Mobile Phone Camera Sensor Controller
Authors: Park Soojin
Published: March 2019

One of the most widely used models for specifying functional requirements is a use case model. The viewpoint of the use case model that views a system as a black box focuses on descriptions of external interactions between the system and related environments. However, for embedded systems that do not disclose most implementation logics outside the system, black box-based use case models may experience the drawback that considerable information that must be defined for system developments is omitted. To solve this shortcoming, several studies have been proposed on the use of kind of white box technique in which the dynamic behaviors of embedded systems are defined first using a state diagram and the results are reflected in the requirement specifications. However, white box-based modeling has not been widely adopted by developers due to tasks that require a lot of time in the requirement analysis phase in the initial phase of the software development life cycle. This study proposes a gray box-based requirement specification method as a trade-off between two contradictory elements (the amount of information required to develop an embedded system and the cost of the effort required during the requirement analysis phase) in terms of the two approaches, the black and the white box-based models. The proposed method suggests that an appropriate depth level of embedded system modeling is required to define the requirements. This study also proposes a mechanism that automatically generates an application programming interface for each component based on the created model. The proposed method was applied to the development of a camera sensor controller in a mobile phone, and the case results proved the feasibility of the method through discussion of the application results.
Experimental Observation of Temperature-Driven Topological Phase Transition in HgTe/CdHgTe Quantum Wells
Authors: Zholudev Maksim S. , Kadykov Aleksandr M. , Fadeev Mikhail A. , Marcinkiewicz Michal , Ruffenach Sandra , Consejo Christophe , Knap Wojciech , Torres Jeremie , Morozov Sergey V. , Gavrilenko Vladimir I. , Mikhailov Nikolay N. , Dvoretskii Sergey A. , Teppe Frederic
Published: March 2019

We report on the comparison between temperature-dependent magneto-absorption and magnetotransport spectroscopy of HgTe/CdHgTe quantum wells in terms of the detection of the phase transition between the topological insulator and band insulator states. Our results demonstrate that temperature-dependent magnetospectroscopy is a powerful tool to discriminate trivial and topological insulator phases, yet the magnetotransport method is shown to have advantages for the clear manifestation of the phase transition with accurate quantitative values of the transition parameter (i.e., critical magnetic field Bc).
GPS Technology for Semi-Aquatic Turtle Research
Authors: Cochrane Madaline M. , Brown Donald J. , Moen Ron A.
Published: March 2019

Global positioning system (GPS) telemetry units are now small enough to be deployed on terrestrial and semi-aquatic turtles. Many of these GPS units use snapshot technology which collects raw satellite and timestamp data during brief periods of data recording to minimize size. We evaluated locations from snapshot GPS units in stationary tests and on wood turtles (Glyptemys insculpta) in northeastern Minnesota. Stationary GPS units were placed in wood turtle habitat to evaluate location accuracy, fix success rate, and directional bias. The GPS fix success rate and accuracy were reduced in closed canopy conditions and when the stationary GPS unit was placed under a log to simulate wood turtle hiding behavior. We removed GPS location outliers and used a moving average calculation to reduce mean location error in stationary tests from 27 m (SD = 38) to 10 m (SD = 8). We then deployed GPS units and temperature loggers on wood turtles and collected 122,657 GPS locations and 242,781 temperature readings from 26 turtles from May to September 2015 and 2016. Location outliers accounted for 12% of locations when the GPS receiver was on a turtle. We classified each wood turtle location based on the GPS location and by comparing temperature profiles from river, sun, and shaded locations to the temperature logger on the turtle. We estimated that wood turtles were on land 68% (SD = 12) of the time from May to September. The fix success rate for land locations was 38% (SD = 9), indicating that wood turtles often use habitats with obstructed views of the sky. Mean net daily movement was 55 m (SD = 192). Our results demonstrate that snapshot GPS units and temperature loggers provide fine-scale GPS data useful in describing spatial ecology and habitat use of semi-aquatic turtles.
The Spatial and Temporal Distribution of Process Gases within the Biowaste Compost
Authors: Stegenta Sylwia , Sobieraj Karolina , Pilarski Grzegorz , Koziel Jacek A. , Białowiec Andrzej
Published: March 2019

Composting is generally accepted as the sustainable recycling of biowaste into a useful and beneficial product for soil. However, composting processes can produce gases that are considered air pollutants. In this dataset, we summarized the spatial and temporal distribution of process gases (including rarely reported carbon monoxide, CO) generated inside full-scale composting piles. In total 1375 cross-sections were made and presented in 230 figures. The research aimed to investigate the phenomenon of gas evolution during the composting of biowaste depending on the pile turning regime (no turning, turning once a week, and turning twice a week) and pile location (outdoors, and indoors in a composting hall). The analyzed biowaste (a mixture of tree leaves and branches, grass clippings, and sewage sludge) were composted in six piles with passive aeration including additional turning at a municipal composting plant. The chemical composition and temperature of process gases within each pile were analyzed weekly for ~49–56 days. The variations in the degree of pile aeration (O2 content), temperature, and the spatial distribution of CO, CO2 and NO concentration during the subsequent measurement cycles were summarized and visualized. The lowest O2 concentrations were associated with the central (core) part of the pile. Similarly, an increase in CO content in the pile core sections was found, which may indicate that CO is oxidized in the upper layer of composting piles. Higher CO and CO2 concentrations and temperature were also observed in the summer season, especially on the south side of piles located outdoors. The most varied results were for the NO concentrations that occurred in all conditions. The dataset was used by the composting plant operator for more sustainable management. Specifically, the dataset allowed us to make recommendations to minimize the environmental impact of composting operations and to lower the risk of worker exposure to CO. The new procedure is as follows: turning of biowaste twice a week for the first two weeks, followed by turning once a week for the next two weeks. Turning is not necessary after four weeks of the process. The recommended surface-to-volume ratio of a compost pile should not exceed 2.5. Compost piles should be constructed with a surface-to-volume ratio of less than 2 in autumn and early spring when low ambient temperatures are common.
Investigating the Effect of Intrinsic Motivation on Alpha Desynchronization Using Sample Entropy
Authors: Phukhachee Tustanah , Maneewongvatana Suthathip , Angsuwatanakul Thanate , Iramina Keiji , Kaewkamnerdpong Boonserm
Published: March 2019

The effect of motivation and attention could play an important role in providing personalized learning services and improving learners toward smart education. These effects on brain activity could be quantified by EEG and open the path to analyze the efficiency of services during the learning process. Many studies reported the appearance of EEG alpha desynchronization during the attention period, resulting in better cognitive performance. Motivation was also found to be reflected in EEG. This study investigated the effect of intrinsic motivation on the alpha desynchronization pattern in terms of the complexity of time series data. The sample entropy method was used to quantify the complexity of event-related spectral perturbation (ERSP) of EEG data. We found that when participants can remember the stimulus, ERSP was significantly less complex than when they cannot. However, the effect of intrinsic motivation cannot be defined by using sample entropy directly. ERSP’s main effect showed that motivation affects the complexity of ERSP data; longer continuous alpha desynchronization patterns were found when participants were motivated. Therefore, we introduced an algorithm to identify the longest continuous alpha desynchronization pattern. The method allowed us to understand that intrinsic motivation has an effect on recognition at the frontal and left parietal area directly.