bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Evidence-Based Practice Competency among Greek Undergraduate Nursing Students
Authors: Miliara Eleni , Kalokairinou Athena , Schetaki Stefania , Patelarou Evridiki , Patelarou Athina
Published: September 2024

For several years, the global scientific community has accepted and recognized the importance of evidence-based practice for nursing science. The main factor for the implementation of evidence-based practice is the competence of undergraduate nursing students towards evidence-based practice, so that they as active nurses are ready for its application in their clinical practice, in order to provide better care for their patients. The aim of the present study is to examine the level of evidence-based practice competency evaluated with the self-reported Evidence-Based Practice Competence Questionnaire. It is important to mention that such a study has not been conducted on nursing students in Greece, but only on registered nurses. A quantitative study based on cross-sectional design was conducted from February to June 2022 in a convenience sample of Greek undergraduate nursing students. The SPSS 26.0 program was used to perform descriptive, bivariate, and multivariate analyses. A total of 175 undergraduate students participated at the Hellenic Mediterranean University Department of Nursing (Greece), specifically from the 2nd and 3rd academic years. The mean score of the Evidence-Based Practice Competence Questionnaire for nursing undergraduate students was 3.03 ± 0.26, indicating moderate evidence-based practice. Among the Evidence-Based Practice Competence Questionnaire dimensions, mean scores of 3.03 ± 0.32 for attitude toward evidence-based practice, 3.01 ± 0.49 for skills in evidence-based practice, and 3.03 ± 0.49 for knowledge in evidence-based practice were obtained. Significant differences among participants’ mean Evidence-Based Practice Competence Questionnaire scores regarding gender (p = 0.766), age (p = 0.400), academic year (p = 0.153), and training in the field of research methodology (p = 0.538) were not found. It appears that the level of readiness towards evidence-based practice is mediocre among undergraduate nursing students at a university in Greece. Therefore, it is necessary to carry out new studies in the future, so that there is a correct approach of all the elements that contribute to the readiness of nursing undergraduate students regarding evidence-based practice.
Soilless Production of Lettuce (Lactuca sativa) in the Atacama Desert Using Fog Water: Water Quality and Produce Mineral Composition
Authors: Albornoz Francisco , Vilches Inés , del Río Camilo , Carter Virginia
Published: September 2024

Soilless vegetable production in the Atacama Desert of Northern Chile is spreading since it is perceived as an alternative that requires much less water than open field soil production. However, strong competition between mining and urban use for human population consumption exists, forcing growers to use alternative water sources. Fog is commonly present in the coastal areas of Northern Chile; however, little information exists with regards to its chemical composition and the effect on nutrient quality of the produce. To address this knowledge gap, a set of experiments was carried out in Chañaral, a small town located in the Atacama Desert of Northern Chile. There, a 200 m2 greenhouse equipped with twenty deep flow pools was used in two consecutive growing cycles. Water for the mixing of the nutrient solution was collected from the fog using fog-catchers and later stored in 2000-L tanks. Fog water quality (electrical conductivity, pH and mineral content) was monitored directly from the storage tanks. Two types of lettuce, green butterhead and red oak leaf, were compared on their yield and accumulation of nutrients and heavy metals. The results indicate that fog water is of good quality for soilless production, with an electrical conductivity value of 0.65 ± 0.18 and low content of heavy metals. Plants’ heavy metal accumulation is below the recommendation of Food and Agriculture Organization and World Health Organization. Fog water presents as a viable water source for soilless production in Northern Chile.
Application Methods of Zinc Sulphate Increased Safflower Seed Yield and Quality under End-Season Drought Stress
Authors: Ahmadi Reza , Mahmoudi Mohammad , Shekari Farid , Afsahi Kamran , Shekari Kiana , Saba Jalal , Mastinu Andrea
Published: September 2024

Zinc deficiency is one of the most widespread nutritional problems, affecting nearly one-third of the world population. In addition, it is known that zinc deficiency not only reduces crop yield but also its quality. The effect of different methods of zinc application on the growth, yield, and quality of safflower seeds under regular irrigation and interruption of irrigation from flowering to harvest (82 and 80 DAS in the first and second years, respectively) was evaluated. Zinc sulfate was applied in both soil and foliar methods. The zinc sulfate treatments include no zinc sulfate, soil application of 20, 40, and 60 kg ha−1 at the planting stage; spraying 2.5, 5, and 7.5 g L−1 in the rosette stage; and spraying 2.5, 5, and 7.5 g L−1 in the flowering stage. The end-season drought caused a decrease in the chlorophyll index, leaf area index, relative water content, plant height, yield components, biological yield, seed yield, harvest index, seed oil content, oil harvest index, and seed element content compared to regular irrigation. The decrease in yield occurred with a decrease in the capitol number and diameter, seed number per capitol, and 1000-seed weight. The severity of the damage of the end-season drought stress in the second year was higher than in the first year due to the higher temperatures and the decrease in the rainfall. In both years, the application of zinc sulfate in different ways had an increasing effect on the studied traits in both normal and stress conditions. The application of zinc sulfate reduced the negative effects of unfavorable environmental conditions and improved the yield and nitrogen, phosphorus, potassium, zinc, and iron element content in the seed. In both application methods of zinc sulfate, the increment in the zinc sulfate concentration decreased the seed phosphorus content. However, the phosphorous content was more than that of the treatment of non-zinc application. The application of zinc increased the biological, seed, and oil yield of the treated plants, but the seed and oil yield were more affected. This effect was shown in the seed and oil harvest index increment. Under regular irrigation, higher concentrations of zinc sulfate enhanced plant performance, but under stress conditions, medium and lower concentrations were more effective. The highest 1000-seed weight and potassium and zinc content were obtained by spraying zinc sulfate at 5 g L−1 in the flowering stage under normal irrigation conditions. A comparison of the two methods of applying zinc sulfate showed that foliar spraying was more effective than soil application in improving the seed yield. The soil application is more effective on biological yield than seed yield.
Determinants of Intra-City Residential Migration Patterns of Older Adults: A GIS and Decision Tree Analysis of Yancheng City, China
Authors: Hou Zhulin , Li Xiangfeng , Li Xiaoming
Published: September 2024

This study investigates the spatial patterns of residential migration among older adults in the city center of Yancheng and the influencing factors using data on the home purchases of individuals aged 65 and older from 2016 to 2018, along with peripheral point of interest (POI) data, analyzed with ArcGIS and a decision tree model. The results indicated that persons aged 60–65 accounted for 42.8% of the total sample and primarily chose to migrate in the early stages of retirement. The intra-city migration of older adults exhibits both centripetal and centrifugal patterns, with a greater tendency toward the city center. House prices, floor levels, and commercial facilities significantly impact their choice of migration destinations. Among these, house prices were the most critical determinant, with the majority of older adults migrating to neighborhoods with lower house prices. This study contributes by integrating residential migration and location choice research and constructing an analytical framework based on facility accessibility. The findings provide insights into the key determinants of location choice for intra-city residential migration among older adults and the construction of livable neighborhoods for them.
Nutraceuticals in Pregnancy: A Special Focus on Probiotics
Authors: Perna Angelica , Venditti Noemi , Merolla Francesco , Fusco Sabato , Guerra Germano , Zoroddu Stefano , De Luca Antonio , Bagella Luigi
Published: September 2024

The placenta is crucial to fetal development and performs vital functions such as nutrient exchange, waste removal and hormone regulation. Abnormal placental development can lead to conditions such as fetal growth restriction, pre-eclampsia and stillbirth, affecting both immediate and long-term fetal health. Placental development is a highly complex process involving interactions between maternal and fetal components, imprinted genes, signaling pathways, mitochondria, fetal sexomes and environmental factors such as diet, supplementation and exercise. Probiotics have been shown to make a significant contribution to prenatal health, placental health and fetal development, with associations with reduced risk of preterm birth and pre-eclampsia, as well as improvements in maternal health through effects on gut microbiota, lipid metabolism, vaginal infections, gestational diabetes, allergic diseases and inflammation. This review summarizes key studies on the influence of dietary supplementation on placental development, with a focus on the role of probiotics in prenatal health and fetal development.
Alzheimer’s Disease as a Membrane Dysfunction Tauopathy? New Insights into the Amyloid Cascade Hypothesis
Authors: Olejar Tomas , Jankovska Nikol , Matej Radoslav
Published: September 2024

The amyloid cascade hypothesis postulates that extracellular deposits of amyloid β (Aβ) are the primary and initial cause leading to the full development of Alzheimer’s disease (AD) with intracellular neurofibrillary tangles; however, the details of this mechanism have not been fully described until now. Our preliminary data, coming from our day-to-day neuropathology practice, show that the primary location of the hyperphosphorylated tau protein is in the vicinity of the cell membrane of dystrophic neurites. This observation inspired us to formulate a hypothesis that presumes an interaction between low-density lipoprotein receptor-related protein 1 (LRP1) and fibrillar aggregates of, particularly, Aβ42 anchored at the periphery of neuritic plaques, making internalization of the LRP1-Aβ42 complex infeasible and, thus, causing membrane dysfunction, leading to the tauopathy characterized by intracellular accumulation and hyperphosphorylation of the tau protein. Understanding AD as a membrane dysfunction tauopathy may draw attention to new treatment approaches not only targeting Aβ42 production but also, perhaps paradoxically, preventing the formation of LRP1-Aβ42.
MicroRNA-27a-5p Downregulates Expression of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Human Dental Pulp Cells via the NF-κB Signaling Pathway
Authors: Wang Shihan , Kawashima Nobuyuki , Han Peifeng , Sunada-Nara Keisuke , Yu Ziniu , Tazawa Kento , Fujii Mayuko , Kieu Thoai Quoc , Okiji Takashi
Published: September 2024

MicroRNA-27a-5p (miR-27a-5p) was significantly upregulated in dental pulp inflammation, yet its underlying mechanisms remain unclear. This study investigated the effect of miR-27a-5p on the expression of proinflammatory cytokines in human dental pulp cells (hDPCs) stimulated by lipopolysaccharide (LPS). LPS-stimulated hDPCs showed concurrent increases in the expression of miR-27a-5p and proinflammatory cytokines (IL-6, IL-8, and MCP1), and the increased expression was suppressed by NF-κB inhibitor BAY 11-0785. Transfection of the miR-27a-5p mimic downregulated the expression of proinflammatory cytokines, NF-κB activity, and the expression of NF-κB signaling activators (TAB1, IRAK4, RELA, and FSTL1) in LPS-stimulated hDPCs. Luciferase reporter assays revealed that miR-27a-5p bound directly to the 3’-UTR of TAB1. siTAB1 downregulated NF-κB activity and proinflammatory cytokine expression. Downregulation of proinflammatory cytokine expression, NF-κB activity, and NF-κB signaling activator expression (TAB1, IRAK4, and RELA) was also found in LPS-stimulated rat incisor pulp tissue explants following transfection with the miR-27a-5p mimic ex vivo. MiR-27a-5p, whose expression was induced by NF-κB signaling, negatively regulated the synthesis of proinflammatory cytokines via targeting NF-κB signaling. In particular, TAB1, a potent NF-κB activator, was targeted by miR-27a-5p. These results provide insights into the negative regulatory effects of miR-27a-5p, particularly those targeting the TAB1-NF-κB signaling pathway, on pulp inflammation.
Clinical and Genetic Insights into Desbuquois Dysplasia: Review of 111 Case Reports
Authors: Piwar Hubert , Ordak Michal , Bujalska-Zadrozny Magdalena
Published: September 2024

Skeletal disorders encompass a wide array of conditions, many of which are associated with short stature. Among these, Desbuquois dysplasia is a rare but severe condition characterized by profound dwarfism, distinct facial features, joint hypermobility with multiple dislocations, and unique vertebral and metaphyseal anomalies. Desbuquois dysplasia is inherited in an autosomal recessive manner, with both the DBQD1 (MIM 251450) and DBQD2 (MIM 615777) forms resulting from biallelic mutations. Specifically, DBQD1 is associated with homozygous or compound heterozygous mutations in the CANT1 gene, while DBQD2 can result from mutations in either the CANT1 or XYLT1 genes. This review synthesizes the findings of 111 published case reports, including 54 cases of DBQD1, 39 cases of DBQD2, and 14 cases of the Kim variant (DDKV). Patients in this cohort had a median birth weight of 2505 g, a median length of 40 cm, and a median occipitofrontal circumference of 33 cm. The review highlights the phenotypic variations across Desbuquois dysplasia subtypes, particularly in facial characteristics, joint dislocations, and bone deformities. Genetic analyses revealed a considerable diversity in mutations, with over 35% of cases involving missense mutations, primarily affecting the CANT1 gene. Additionally, approximately 60% of patients had a history of parental consanguinity, indicating a potential genetic predisposition in certain populations. The identified mutations included deletions, insertions, and nucleotide substitutions, many of which resulted in premature stop codons and the production of truncated, likely nonfunctional proteins. These findings underscore the genetic and clinical complexity of Desbuquois dysplasia, highlighting the importance of early diagnosis and the potential for personalized therapeutic approaches. Continued research is essential to uncover the underlying mechanisms of this disorder and improve outcomes for affected individuals through targeted treatments.
Molecular Profile as an Outcome Predictor in Glioblastoma along with MRI Features and Surgical Resection: A Scoping Review
Authors: Papacocea Serban Iancu , Vrinceanu Daniela , Dumitru Mihai , Manole Felicia , Serboiu Crenguta , Papacocea Marius Toma
Published: September 2024

Glioblastoma (GBM) is one of the most aggressive malignant tumors of the brain. We queried PubMed for articles about molecular predictor markers in GBM. This scoping review aims to analyze the most important outcome predictors in patients with GBM and to compare these factors in terms of absolute months of survival benefit and percentages. Performing a gross total resection for patients with GBM undergoing optimal chemo- and radiotherapy provides a significant benefit in overall survival compared to those patients who received a subtotal or partial resection. However, compared to IDH-Wildtype GBMs, patients with IDH-Mutant 1/2 GBMs have an increased survival. MGMT promoter methylation status is another strong outcome predictor for patients with GBM. In the reviewed literature, patients with methylated MGMT promoter lived approximately 50% to 90% longer than those with an unmethylated MGMT gene promoter. Moreover, KPS is an important predictor of survival and quality of life, demonstrating that we should refrain from aggressive surgery in important brain areas. As new therapies (such as TTFs) emerge, we are optimistic that the overall median survival will increase, even for IDH-Wildtype GBMs. In conclusion, molecular profiles are stronger outcome predictors than the extent of neurosurgical resection for GBM.
Antimicrobial Activity of Chalcones with a Chlorine Atom and Their Glycosides
Authors: Krawczyk-Łebek Agnieszka , Żarowska Barbara , Janeczko Tomasz , Kostrzewa-Susłow Edyta
Published: September 2024

Chalcones, secondary plant metabolites, exhibit various biological properties. The introduction of a chlorine and a glucosyl substituent to the chalcone could enhance its bioactivity and bioavailability. Such compounds can be obtained through a combination of chemical and biotechnological methods. Therefore, 4-chloro-2′-hydroxychalcone and 5′-chloro-2′-hydroxychalcone were obtained by synthesis and then glycosylated in two filamentous fungi strains cultures, i.e., Isaria fumosorosea KCH J2 and Beauveria bassiana KCH J1.5. The main site of the glycosylation of both compounds by I. fumosorosea KCH J2 was C-2′ and C-3 when the second strain was utilized. The pharmacokinetics of these compounds were predicted using chemoinformatics tools. Furthermore, antimicrobial activity tests were performed. Compounds significantly inhibited the growth of the bacteria strains Escherichia coli 10536, Staphylococcus aureus DSM 799, and yeast Candida albicans DSM 1386. Nevertheless, the bacterial strain Pseudomonas aeruginosa DSM 939 exhibited significant resistance to their effects. The growth of lactic acid bacteria strain Lactococcus acidophilus KBiMZ 01 bacteria was moderately inhibited, but strains Lactococcus rhamnosus GG and Streptococcus thermophilus KBM-1 were completely inhibited. In summary, chalcones substituted with a chlorine demonstrated greater efficacy in inhibiting the microbial strains under examination compared to 2′-hydroxychalcone, while aglycones and their glycosides exhibited similar effectiveness.
Distinct Variations in Gene Expression and Cell Composition across Lichen Planus Subtypes
Authors: Knoch Cadri , Baghin Veronika , Turko Patrick , Winkelbeiner Nicola , Staeger Ramon , Wei Kongchang , Banzola Irina , Mellett Mark , Levesque Mitchell P. , Kuendig Thomas , French Lars E. , Heinzerling Lucie , Meier-Schiesser Barbara
Published: September 2024

Lichen planus (LP) is a highly prevalent inflammatory skin disease. While various clinical subtypes have been defined, detailed comparisons of these variants are lacking. This study aimed to elucidate differences in gene expression and cellular composition across LP subtypes. Lesional skin biopsies from 28 LP patients (classical, oral, genital, and lichen planopilaris) and seven non-diseased skin controls (NDC) were analyzed. Gene expression profiling of 730 inflammation-related genes was conducted using NanoString. Immune cell compositions were assessed by multiplex immunohistochemistry. Gene expression profiles revealed unique inflammatory signatures for each LP subtype. Lichen planopilaris exhibited the most divergence, with downregulated gene expression and upregulation of complement pathway genes (C5-7), along with elevated M2 macrophages. Oral and genital LP demonstrated similar profiles with strong upregulation of TNF-related and Toll-like receptor-associated genes. Oral LP showed the highest upregulation of cytotoxicity-associated genes, as well as high numbers of CD8+ IL-17A+ (Tc17) cells (8.02%). Interferon gene signatures were strongly upregulated in oral and classical LP. The study highlights distinct differences in inflammatory gene expression and cell composition across LP subtypes, emphasizing the need for tailored therapeutic approaches.
Oral Health and Nutraceutical Agents
Authors: Leo Mariantonietta , D’Angeli Floriana , Genovese Carlo , Spila Antonella , Miele Chiara , Ramadan Dania , Ferroni Patrizia , Guadagni Fiorella
Published: September 2024

Oral health is essential for both overall health and quality of life. The mouth is a window into the body’s health, and nutrition can strongly impact the state of general and oral health. A healthy diet involves the synergistic effect of various nutraceutical agents, potentially capable of conferring protective actions against some inflammatory and chronic-degenerative disorders. Nutraceuticals, mostly present in plant-derived products, present multiple potential clinical, preventive, and therapeutic benefits. Accordingly, preclinical and epidemiological studies suggested a protective role for these compounds, but their real preventive and therapeutic effects in humans still await confirmation. Available evidence suggests that plant extracts are more effective than individual constituents because they contain different phytochemicals with multiple pharmacological targets and additive/synergistic effects, maximizing the benefits for oral health. Moreover, nutritional recommendations for oral health should be personalized and aligned with valid suggestions for overall health. This review is aimed to: introduce the basic concepts of nutraceuticals, including their main food sources; examine the logic that supports their relationship with oral health, and summarize and critically discuss clinical trials testing the utility of nutraceuticals in the prevention and treatment of oral diseases.
Neutrophil Biomarkers Can Predict Cardiotoxicity of Anthracyclines in Breast Cancer
Authors: Todorova Valentina K. , Azhar Gohar , Stone Annjanette , Malapati Sindhu J. , Che Yingni , Zhang Wei , Makhoul Issam , Wei Jeanne Y.
Published: September 2024

Doxorubicin (DOX), a commonly used anticancer agent, causes cardiotoxicity that begins with the first dose and may progress to heart failure years after treatment. An inflammatory response associated with neutrophil recruitment has been recognized as a mechanism of DOX-induced cardiotoxicity. This study aimed to validate mRNA expression of the previously identified biomarkers of DOX-induced cardiotoxicity, PGLYRP1, CAMP, MMP9, and CEACAM8, and to assay their protein expression in the peripheral blood of breast cancer patients. Blood samples from 40 breast cancer patients treated with DOX-based chemotherapy were collected before and after the first chemotherapy cycle and > 2 years after treatment. The protein and gene expression of PGLYRP1/Tag7, CAMP/LL37, MMP9/gelatinase B, and CEACAM8/CD66b were determined using ELISA and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) curve analysis was used to determine the diagnostic value of each candidate biomarker. Patients with cardiotoxicity (n = 20) had significantly elevated levels of PGLYRP1, CAMP, MMP9, and CEACAM8 at baseline, after the first dose of DOX-based chemotherapy, and at > 2 years after treatment relative to patients without cardiotoxicity (n = 20). The first dose of DOX induced significantly higher levels of all examined biomarkers in both groups of patients. At > 2 years post treatment, the levels of all but MMP9 dropped below the baseline. There was a good correlation between the expression of mRNA and the target proteins. We demonstrate that circulating levels of PGLYRP1, CAMP, MMP9, and CEACAM8 can predict the cardiotoxicity of DOX. This novel finding may be of value in the early identification of patients at risk for cardiotoxicity.
Experimental Cell Models for Investigating Neurodegenerative Diseases
Authors: Evangelisti Cecilia , Ramadan Sherin , Orlacchio Antonio , Panza Emanuele
Published: September 2024

Experimental models play a pivotal role in biomedical research, facilitating the understanding of disease mechanisms and the development of novel therapeutics. This is particularly true for neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis, and motor neuron disease, which present complex challenges for research and therapy development. In this work, we review the recent literature about experimental models and motor neuron disease. We identified three main categories of models that are highly studied by scientists. In fact, experimental models for investigating these diseases encompass a variety of approaches, including modeling the patient’s cell culture, patient-derived induced pluripotent stem cells, and organoids. Each model offers unique advantages and limitations, providing researchers with a range of tools to address complex biological questions. Here, we discuss the characteristics, applications, and recent advancements in terms of each model system, highlighting their contributions to advancing biomedical knowledge and translational research.
Transcriptome Analysis Reveals the Effect of Oyster Mushroom Spherical Virus Infection in Pleurotus ostreatus
Authors: Wang Yifan , Yan Junjie , Song Guoyue , Song Zhizhong , Shi Matthew , Hu Haijing , You Lunhe , Zhang Lu , Wang Jianrui , Liu Yu , Cheng Xianhao , Zhang Xiaoyan
Published: September 2024

Oyster mushroom spherical virus (OMSV) is a mycovirus that inhibits mycelial growth, induces malformation symptoms, and decreases the yield of fruiting bodies in Pleurotus ostreatus. However, the pathogenic mechanism of OMSV infection in P. ostreatus is poorly understood. In this study, RNA sequencing (RNA-seq) was conducted, identifying 354 differentially expressed genes (DEGs) in the mycelium of P. ostreatus during OMSV infection. Verifying the RNA-seq data through quantitative real-time polymerase chain reaction on 15 DEGs confirmed the consistency of gene expression trends. Both Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses highlighted the pivotal role of primary metabolic pathways in OMSV infection. Additionally, significant changes were noted in the gene expression levels of carbohydrate-active enzymes (CAZymes), which are crucial for providing the carbohydrates needed for fungal growth, development, and reproduction by degrading renewable lignocellulose. The activities of carboxymethyl cellulase, laccase, and amylase decreased, whereas chitinase activity increased, suggesting a potential mechanism by which OMSV influenced mycelial growth through modulating CAZyme activities. Therefore, this study provided insights into the pathogenic mechanisms triggered by OMSV in P. ostreatus.
Enhancing Flight Delay Predictions Using Network Centrality Measures
Authors: Ajayi Joseph , Xu Yao , Li Lixin , Wang Kai
Published: September 2024

Accurately predicting flight delays remains a significant challenge in the aviation industry due to the complexity and interconnectivity of its operations. The traditional prediction methods often rely on meteorological conditions, such as temperature, humidity, and dew point, as well as flight-specific data like departure and arrival times. However, these predictors frequently fail to capture the nuanced dynamics that lead to delays. This paper introduces network centrality measures as novel predictors to enhance the binary classification of flight arrival delays. Additionally, it emphasizes the use of tree-based ensemble models, specifically random forest, gradient boosting, and CatBoost, which are recognized for their superior ability to model complex relationships compared to single classifiers. Empirical testing shows that incorporating centrality measures improves the models’ average performance, with random forest being the most effective, achieving an accuracy rate of 86.2%, surpassing the baseline by 1.7%.
Mechanical Performance of a Hot Mix Asphalt Modified with Biochar Obtained from Oil Palm Mesocarp Fiber
Authors: Chaves-Pabón Saieth Baudilio , Rondón-Quintana Hugo Alexander , Bastidas-Martínez Juan Gabriel
Published: September 2024

A recently used material that shows environmental and technical advantages for use as an asphalt binder modifier is biochar (BC). Different biomasses can be converted into BC by pyrolysis. One agro-industrial biomass that is abundant in copious quantities is oil palm mesocarp fiber (OPMF) obtained from African palm cultivation. In the present study, the use of a BC obtained from OPMF (BC-OPMF) as a modifier of asphalt binder (AC type) to produce a hot mix asphalt (HMA) was evaluated. This type of BC has not been investigated or reported in the reference literature as a binder and/or asphalt mix modifier. Initially, AC was modified with BC in three ratios (BC/AC = 5, 10, and 15%, with respect to mass) to perform penetration, softening point, and rotational viscosity tests; rheological characterization at high and intermediate temperatures; and scanning electron microscope (SEM) visualization. Based on this experimental phase, BC/AC = 10% was chosen to manufacture the modified HMA. Resistance parameters under monotonic loading (stability—S, flow—F, S/F ratio of the Marshall test, and indirect tensile strength in dry—ITSD and wet—ITSC conditions) and cyclic loading (resilient modulus, permanent deformation, and fatigue resistance under stress-controlled conditions) were evaluated on the control HMA (AC unmodified) and the modified HMA. Additionally, the tensile strength ratio (TSR) was calculated to evaluate the resistance to moisture damage. Abrasion and raveling resistance were evaluated by performing Cantabro tests. BC-OPMF is shown to be a sustainable and promising material for modifying asphalt binders for those seeking to increase stiffness and rutting resistance in high-temperature climates, resistance to moisture damage, raveling, and fatigue without increasing the optimum asphalt binder content (OAC), changing the volumetric composition of the HMA or increasing the manufacturing and construction temperatures.
Significant Preoperative Anxiety Associated with Perceived Risk and Gender in Cataract Surgery
Authors: Floros Georgios , Kandarakis Stylianos , Glynatsis Nikolaos , Glynatsis Filaretos , Mylona Ioanna
Published: September 2024

Background/Objectives: Cataract surgery is an often-sought solution to the universal problem of lens opacification. Studies of perioperative anxiety have yielded conflicting results, reporting a high prevalence but low clinical significance. The objective of this study was to ascertain anxiety levels immediately after the scheduling of surgery, controlling for trait anxiety and other related factors. Methods: This is an observational comparative study of two patient populations assessed for receiving cataract surgery, with one group of seventy patients scheduled for operation and receiving an assessment of the potential perioperative risk and the other group of seventy patients deemed ineligible for operation since their opacification was not advanced. The patients were assessed for state and trait anxiety while controlling for cognitive status. Results: The findings indicate a clinically significant burden of state anxiety in the group of patients scheduled for operation, with 34 out of 70 meeting the threshold for clinically significant levels of state anxiety compared to 9 out of the 70 patients who were not assigned for surgery (p < 0.001). Those patients who were assigned for surgery were assessed for perioperative risk factors, and state anxiety differed statistically significantly between the preoperative risk factor groups, (p = 0.003) with those assessed as having at least low perioperative risk presenting with more anxiety than those without any risk factors. Male patients exhibited lower state anxiety compared to female patients in the group assigned to surgery (p = 0.028). Cognitive status did not affect the results. Conclusions: These findings point to the importance of prevention against perioperative anxiety early on, especially in patients with a higher perioperative risk and female gender.
The Impact of Tumor Elongation on Facial Nerve Outcome after Surgery for Koos Grade 3 and 4 Vestibular Schwannomas in the Semi-Sitting Position via the Retrosigmoid Approach
Authors: Glieme Franziska , Haddad Lisa , Arlt Felix , Vychopen Martin , Seidel Clemens , Barrantes-Freer Alonso , Güresir Erdem , Wach Johannes
Published: September 2024

Background: Facial nerve paralysis is a severe dysfunction after vestibular schwannoma (VS) surgery. Methods: This monocentric study analyzed 61 patients who underwent sporadic VS surgery in a standardized manner. The primary endpoint was the facial nerve outcome (FNO) at 3 months after VS surgery. FNO was dichotomized into “good” (House–Brackmann (HB) score ≤ 2) and “poor” (HB > 2). Results: Poor FNO was observed in 11 patients (18.0%) at 3 months after VS surgery. Radiomic tumor shape features were analyzed, and the AUC of elongation in the prediction of a poor HB at 3 months was 0.70 (95% CI: 0.56–0.85, p = 0.03) and the optimum threshold value (≤/>0.35) yielded a sensitivity and specificity of 64.0% and 75.4%, respectively. Multivariable logistic regression analyses considering the extent of resection (</≥93.4%), preoperative tumor volume (</≥2.6 cm3), age (</≥55), sex (female/male), and elongation (≤/>0.35) revealed that more elongated VSs (≤0.35; OR: 5.8; 95%CI: 1.2–28.2; p = 0.03) and those with an increased EoR (≥93.4%; OR: 6.5; 95%CI: 1.0–42.5; p = 0.05) are independently associated with poorer FNO at 3 months after surgery. Conclusions: Highly elongated VS shape seems to be a risk factor for worsened facial nerve outcome at 3 months after surgery for Koos grade 3 and 4 tumors.
Effectiveness of Laser Therapy in Treatment of Temporomandibular Joint and Muscle Pain
Authors: Buduru Smaranda , Oprea Denisa Maria , Manziuc Manuela-Maria , Leucuța Daniel-Corneliu , Almășan Oana
Published: September 2024

Background/Objectives: Temporomandibular joint disorders (TMDs) express a condition derived from a broad spectrum of etiological factors and clinical manifestations. Many treatment options have been developed for TMDs; nevertheless, conservative and non-invasive approaches ought to be prioritized. Laser therapy is an effective treatment for pain management due to its non-invasive nature and capacity for tissue regeneration. This review aimed at bringing an overview of the present evidence regarding the efficiency of laser therapy on myofascial or temporomandibular joint disorders pain. Methods: The search was conducted in four electronic databases: PubMed, Web of Science, Embase, and Scopus, of studies published between January 1997 and January 2023. The following terms have been extensively searched: “laser treatment”, pain management”, “temporomandibular joint disorders”, “masseter muscle pain”, “pterygoid muscle pain”, and “temporal muscle pain”. The inclusion criteria were original papers, available in full text, and written in English. Cohen’s Kappa coefficient was used to assess the inter-rater reliability for article selection. The methodological quality was assessed with the Cochrane Risk of Bias tool for randomized controlled trials and the National Heart, Lung, and Blood Institute’s quality assessment tool for before-after studies with no control group. Results: Out of 846 identified records, 7 studies were included, of which 5 were randomized controlled trials. The inter-rater reliability for article selection showed an almost perfect agreement (Cohen’s Kappa = 0.832, p < 0.001). The protocol of laser application was not standardized; the laser wavelength ranged from 633 to 940 nm, with a power output range from 25 to 1600 mW. The number of sessions varied from 3 to 12, with a frequency of application from 1 time per week to 3 times per week. All studies reported pain reduction after laser therapy. Conclusions: Laser therapy is an efficient method to treat TMDs related to muscle pain. To accomplish the desired results, a standard procedure must be followed; however, the protocol is still not fully designed.
Anatomical Posterior Acetabular Plate Versus Conventional Reconstruction Plates for Acetabular Posterior Wall Fractures: A Comparative Study
Authors: Chuang Chang-Han , Chuang Hao-Chun , Wang Jou-Hua , Yang Jui-Ming , Wu Po-Ting , Hu Ming-Hsien , Su Hong-Lin , Lee Pei-Yuan
Published: September 2024

Background: Functional recovery following the surgical fixation of acetabular posterior wall fractures remains a challenge. This study compares outcomes of posterior wall fracture reconstruction using an anatomical posterior acetabular plate (APAP) versus conventional reconstruction plates. Methods: Forty patients with acetabular fractures involving the posterior wall or column underwent surgery, with 20 treated using APAPs (APAP group) and 20 with conventional pelvic reconstruction plates (control group). Baseline patient characteristics, intraoperative blood loss and time, reduction quality, postoperative function, and postoperative complications were compared using appropriate non-parametric statistical tests. A general linear model for repeated measures analysis of variance was employed to analyze trends in functional recovery. Results: No significant differences were observed in baseline characteristics. APAP significantly reduced surgical time by 40 min (186.5 ± 51.0 versus 225.0 ± 47.7, p =0.004) and blood loss (695 ± 393 versus 930 ± 609, p = 0.049) compared to conventional plates. At 3 and 6 months following surgery, the APAP group exhibited higher functional scores (modified Merle d’Aubigné scores 10 ± 1.8 versus 7.8 ± 1.4, p < 0.001; 13.4 ± 2.8 versus 10.1 ± 2.1, p = 0.001), converging with the control group by 12 months (modified Merle d’Aubigné scores 14.2 ± 2.6 versus 12.7 ± 2.6, p = 0.072; OHS 31.6 ± 12.3 versus 30.3 ± 10.1, p = 0.398). Radiologically, the APAP group demonstrated superior outcomes (p = 0.047). Complication and conversion rates to hip arthroplasty did not significantly differ between groups (10% versus 15%, p = 0.633). Conclusions: The use of an APAP in reconstructing the posterior acetabulum significantly reduces surgical time, decreases intraoperative blood loss, and leads to earlier functional recovery compared to conventional reconstruction plates. The APAP provides stable fixation of the posterior wall and ensures the durable maintenance of reduction, ultimately yielding favorable surgical outcomes.
Brain and Serum Membrane Vesicle (Exosome) Profiles in Experimental Alcohol-Related Brain Degeneration: Forging the Path to Non-Invasive Liquid Biopsy Diagnostics
Authors: De La Monte Suzanne M. , Yang Yiwen , Tong Ming
Published: September 2024

Background: Alcohol-related brain degeneration (ARBD) is associated with cognitive–motor impairments that can progress to disability and dementia. White matter (WM) is prominently targeted in ARBD due to chronic neurotoxic and degenerative effects on oligodendrocytes and myelin. Early detection and monitoring of WM pathology in ARBD could lead to therapeutic interventions. Objective: This study examines the potential utility of a non-invasive strategy for detecting WM ARBD using exosomes isolated from serum. Comparative analyses were made with paired tissue (Tx) and membrane vesicles (MVs) from the temporal lobe (TL). Methods: Long Evans rats were fed for 8 weeks with isocaloric liquid diets containing 37% or 0% caloric ethanol (n = 8/group). TL-Tx, TL-MVs, and serum exosomes (S-EVs) were used to examine ethanol’s effects on oligodendrocyte glycoprotein, astrocyte, and oxidative stress markers. Results: Ethanol significantly decreased the TL-Tx expression of platelet-derived growth factor receptor alpha (PDGFRA), 2′,3′-cyclic nucleotide 3′ phosphodiesterase (CNPase), proteolipid protein (PLP), myelin oligodendrocyte glycoprotein (MOG), glial fibrillary acidic protein (GFAP), and 8-OHdG, whereas in the TL-MVs, ethanol increased CNPase, PDGFRA, and 8-OHdG, but decreased MOG and GFAP concordantly with TL-Tx. Ethanol modulated the S-EV expression by reducing PLP, nestin, GFAP, and 4-hydroxynonenal (HNE). Conclusion: Chronic ethanol exposures differentially alter the expression of oligodendrocyte/myelin, astrocyte, and oxidative stress markers in the brain, brain MVs, and S-EVs. However, directionally concordant effects across all three compartments were limited. Future studies should advance these efforts by characterizing the relationship between ABRD and molecular pathological changes in brain WM-specific exosomes in serum.
Mechanisms of Proppant Transport in Rough Fractures of Offshore Unconventional Reservoirs: Shale and Tight Sandstone
Authors: Yin Biao , Lou Yishan , Liu Shanyong , Xu Peng
Published: September 2024

After hydraulic fracturing, unconventional reservoirs frequently encounter challenges related to limited effective proppant support distance and suboptimal proppant placement. Due to the strong heterogeneity of offshore reservoirs, which causes varying fracture roughnesses depending on different lithologies, a systematic study of the relationship between roughness and proppant transport could optimize operational parameters. This study incorporates the box dimension method for fractal dimension analysis to quantify roughness in auto-correlated Gaussian distributed surfaces created by true triaxial tests. Combined with the numerical analysis of (computational fluid dynamics) CFD-DEM (discrete element method) for bidirectional coupling, the laws of proppant deposition and transport processes within fractures with different roughnesses are obtained through comparative verification simulations. The results show that for rougher fractures of shale, the proppants are transported farther, but at JRC_52, (joint roughness coefficient), where there may be plugging in curved areas, there is a risk of near-well blockages. Compared to the smooth model, fluctuations in JRC_28 (tight sandstone) drastically increase turbulent kinetic energy within the fracture, altering particle transport dynamics. Moreover, smaller proppants (d/w ≤ 0.3) exhibit better transport capacity due to gravity, but the conductivity of the proppant is limited when the particles are too small. A d/w of 0.4 is recommended to guarantee transport capacity and proppant efficiency near the well. Additionally, proppants injected sequentially from small to large in shale fractures offer optimal propping effects, and can take advantage of the better transport capacity of smaller proppants in rough fractures. The large proppant (d/w = 0.8) is primarily deposited by gravity and forms a sloping sand bed, which subsequently ensures the aperture of the fractures. This research provides a fresh perspective on the influence of fracture roughness on proppant transport in offshore unconventional reservoirs and offers valuable considerations for the order of proppant injection.
Exploring Innovative Methods in Maritime Simulation: A Ship Path Planning System Utilizing Virtual Reality and Numerical Simulation
Authors: Li Bing , Li Mingze , Qi Zhigang , Li Jiashuai , Wu Jiawei , Wang Qilong
Published: September 2024

In addressing the high costs, inefficiencies, and limitations of purely digital simulations in maritime trials for unmanned vessel path planning, this paper introduces a ship virtual path planning simulation test system. This system, unbound by temporal and spatial constraints, vividly showcases the navigational performance of vessels. After analyzing the virtual testing requirements for the autonomous navigation performance of unmanned surface vehicles (USVs), we established the overall framework of this system. Data-driven by a numerical simulation platform, the system achieves synchronized operation between physical and virtual platforms and supports interactive path planning simulations between USVs and the virtual testing system. Furthermore, to address the limitations of traditional ship trajectory planning evaluation, this paper develops a global path planning fitness evaluation function that comprehensively considers trajectory safety, navigation distance, and vessel stability, achieving optimal comprehensive routes through the particle swarm optimization algorithm. Test results indicate an average roll reduction of 14.31% in the planned routes, with a slight increase in navigation distance. By integrating two-dimensional curve simulation with three-dimensional visualization, this paper not only overcomes the limitations of purely physical and purely virtual simulations but also enhances the overall credibility and intuitiveness of the simulation. Experimental results validate the system’s effectiveness, providing a novel method for autonomous navigation testing and evaluation of USVs.
Real-Time Prediction of Multi-Degree-of-Freedom Ship Motion and Resting Periods Using LSTM Networks
Authors: Chen Zhanyang , Liu Xingyun , Ji Xiao , Gui Hongbin
Published: September 2024

This study presents a novel real-time prediction technique for multi-degree-of-freedom ship motion and resting periods utilizing Long Short-Term Memory (LSTM) networks. The primary objective is to enhance the safety and efficiency of shipborne helicopter landings by accurately predicting heave, pitch, and roll data over an 8 s forecast horizon. The proposed method utilizes the LSTM network’s capability to model complex nonlinear time series while employing the User Datagram Protocol (UDP) to ensure efficient data transmission. The model’s performance was validated using real-world ship motion data collected across various sea states, achieving a maximum prediction error of less than 15%. The findings indicate that the LSTM-based model provides reliable predictions of ship resting periods, which are crucial for safe helicopter operations in adverse sea conditions. This method’s capability to provide real-time predictions with minimal computational overhead highlights its potential for broader applications in marine engineering. Future research should explore integrating multi-model fusion techniques to enhance the model’s adaptability to rapidly changing sea conditions and improve the prediction accuracy.
Experimental and Simulation Study on Flow-Induced Vibration of Underwater Vehicle
Authors: Zou Yucheng , Du Yuan , Zhao Zhe , Pang Fuzhen , Li Haichao , Hui David
Published: September 2024

At high speeds, flow-induced vibration noise is the main component of underwater vehicle noise. The turbulent fluctuating pressure is the main excitation source of this noise. It can cause vibration of the underwater vehicle’s shell and eventually radiate noise outward. Therefore, by reducing the turbulent pressure fluctuation or controlling the vibration of the underwater vehicle’s shell, the radiation noise of the underwater vehicle can be effectively reduced. This study designs a cone–column–sphere composite structure. Firstly, the effect of fluid–structure coupling on pulsating pressure is studied. Next, a machine learning method is used to predict the turbulent pressure fluctuations and the fluid-induced vibration response of the structure at different speeds. The results were compared with experimental and numerical simulation results. The results show that the deformation of the structure will affect the flow field distribution and pulsating pressure of the cylindrical section. The machine learning method based on the BP (back propagation) neural network model can quickly predict the pulsating pressure and vibration response of the cone–cylinder–sphere composite structure under different Reynolds numbers. Compared with the experimental results, the error of the machine learning prediction results is less than 7%. The research method proposed in this paper provides a new solution for the rapid prediction and control of hydrodynamic vibration noise of underwater vehicles.