bluuebunny/crossref_metadata_2025 huggingface dataset:
Platelet-Rich Plasma (PRP) and Adipose-Derived Stem Cell (ADSC) Therapy in the Treatment of Genital Lichen Sclerosus: A Comprehensive Review
Authors: Paganelli Alessia , Contu Luca , Condorelli Alessandra , Ficarelli Elena , Motolese Alfonso , Paganelli Roberto , Motolese Alberico
Published: November 2023

Lichen sclerosus (LS) is a chronic inflammatory dermatosis mostly localized in the genital area, characterized by vulvar alterations that can severely impact a patient’s quality of life. Current treatment modalities often provide incomplete relief, and there is a need for innovative approaches to manage this condition effectively. Platelet-rich plasma (PRP) and adipose-derived stem cells (ADSCs) have emerged as potential regenerative therapies for LS, offering promising results in clinical practice. This comprehensive review explores the utilization of PRP and ADSC therapy in the treatment of genital LS, highlighting their mechanisms of action, safety profiles, and clinical outcomes. PRP is a blood product enriched in growth factors and cytokines, which promotes tissue regeneration, angiogenesis, and immune modulation. ADSC regenerative potential relies not only in their plasticity but also in the secretion of trophic factors, and modulation of the local immune response. Numerous studies have reported the safety of PRP and ADSC therapy for genital LS. Adverse events are minimal and typically involve mild, self-limiting symptoms, such as transient pain and swelling at the injection site. Long-term safety data are encouraging, with no significant concerns identified in the literature. PRP and ADSC therapy have demonstrated significant improvements in LS-related symptoms, including itching, burning, dyspareunia, and sexual function. Additionally, these therapies enable many patients to discontinue the routine use of topical corticosteroids. Several studies have explored the efficacy of combining PRP and ADSC therapy for LS. In combination, PRP and ADSCs seem to offer a synergistic approach to address the complex pathophysiology of LS, particularly in the early stages. The use of PRP and ADSC therapy for genital lichen sclerosus represents a promising and safe treatment modality. These regenerative approaches have shown significant improvements in LS-related symptoms, tissue trophism, and histological features. Combination therapy, which harnesses the synergistic effects of PRP and ADSCs, is emerging as a preferred option, especially in early-stage LS cases. Further research, including randomized controlled trials and long-term follow-up, is warranted to elucidate the full potential and mechanisms of PRP and ADSC therapy in the management of genital LS. These regenerative approaches hold great promise in enhancing the quality of life of individuals suffering from this challenging condition.
Novel Peritoneal Sclerosis Rat Model Developed by Administration of Bleomycin and Lansoprazole
Authors: Kunitatsu Kosei , Yamamoto Yuta , Nasu Shota , Taniji Akira , Kawashima Shuji , Yamagishi Naoko , Ito Takao , Inoue Shigeaki , Kanai Yoshimitsu
Published: November 2023

In our preliminary experiment, peritoneal sclerosis likely induced by peritoneal dialysis was unexpectedly observed in the livers of rats given bleomycin and lansoprazole. We examined whether this peritoneal thickening around the liver was time-dependently induced by administration of both drugs. Male Wistar rats were injected with bleomycin and/or lansoprazole for 2 or 4 weeks. The 3YB-1 cell line derived from rat fibroblasts was treated by bleomycin and/or lansoprazole for 24 h. The administration of both drugs together, but not individually, thickened the peritoneal tissue around the liver. There was accumulation of collagen fibers, macrophages, and eosinophils under mesothelial cells. Expressions of Col1a1, Mcp1 and Mcp3 genes were increased in the peritoneal tissue around the liver and in 3YB-1 cells by the administration of both drugs together, and Opn genes had increased expressions in this tissue and 3YB-1 cells. Mesothelial cells indicated immunoreactivity against both cytokeratin, a mesothelial cell marker, and αSMA, a fibroblast marker, around the livers of rats given both drugs. Administration of both drugs induced the migration of macrophages and eosinophils and induced fibrosis associated with the possible activation of fibroblasts and the possible promotion of the mesothelial–mesenchymal transition. This might become a novel model of peritoneal sclerosis for peritoneal dialysis.
Metastatic Penile Squamous Cell Carcinoma Responsive to Enfortumab Vedotin
Authors: Fahey Catherine C. , Nebhan Caroline A. , York Sally , Davis Nancy B. , Hurley Paula J. , Gordetsky Jennifer B. , Schaffer Kerry R.
Published: November 2023

Penile squamous cell carcinoma is a rare disease with very limited data to guide treatment decisions. In particular, there is minimal evidence for effective therapies in the metastatic setting. Here, we present a case of metastatic penile squamous cell carcinoma with response to the Nectin-4 inhibitor enfortumab-vedotin-ejfv (EV). EV was selected due to the evidence of the high expression of Nectin-4 in squamous cell carcinomas, including penile carcinoma. The patient had both radiographic and symptomatic improvement after two cycles of treatment, despite having been treated with multiple prior lines of traditional chemotherapy. This case provides support for the use of antibody–drug conjugates (ADC), including EV, in this disease with few other options in the advanced setting. Further studies examining Nectin-4 and ADCs in penile squamous cell carcinoma should be completed, as high-quality evidence is needed to guide treatment after initial progression for these patients.
Notch Signaling in Health and Disease
Authors: Tricarico Paola Maura , Crovella Sergio
Published: November 2023

The Notch signaling pathway, a vital and evolutionarily conserved regulator of cellular processes, intricately shapes both health and disease [...]
Adjuvant Effect of Cinnamon Polyphenolic Components in Colorectal Cancer Cell Lines
Authors: Palmioli Alessandro , Forcella Matilde , Oldani Monica , Angotti Irene , Sacco Grazia , Fusi Paola , Airoldi Cristina
Published: November 2023

Colorectal cancer (CRC) is the second-leading cause of cancer death, with a worldwide incidence rate constantly increasing; thus, new strategies for its prevention or treatment are needed. Here, we describe the adjuvant effect of the polyphenol-enriched fractions of cinnamon, from cinnamon bark and buds, when co-administered with a potent anticancer drug, cetuximab, used for CRC therapy. The co-administration significantly reduces the cetuximab dose required for the antiproliferative activity against colorectal cancer cell line E705, which is sensitive to EGFR-targeted therapy. The anticancer activity of these cinnamon-derived fractions, whose major components (as assessed by UPLC–HRMS analysis) are procyanidins and other flavonoids, strictly correlates with their ability to induce apoptosis in cancer cell lines through ERK activation and the mitochondrial membrane potential impairment. Due to the severe side effects of cetuximab administration, our results suggest the use of nutraceuticals based on the polyphenolic fractions of cinnamon extracts as adjuvants in the therapy of CRC.
Anti-Proliferative Properties of the Novel Hybrid Drug Met-ITC, Composed of the Native Drug Metformin with the Addition of an Isothiocyanate H2S Donor Moiety, in Different Cancer Cell Lines
Authors: Citi Valentina , Barresi Elisabetta , Piragine Eugenia , Spezzini Jacopo , Testai Lara , Da Settimo Federico , Martelli Alma , Taliani Sabrina , Calderone Vincenzo
Published: November 2023

Metformin (Met) is the first-line therapy in type 2 diabetes mellitus but, in last few years, it has also been evaluated as anti-cancer agent. Several pathways, such as AMPK or PI3K/Akt/mTOR, are likely to be involved in the anti-cancer Met activity. In addition, hydrogen sulfide (H2S) and H2S donors have been described as anti-cancer agents affecting cell-cycle and inducing apoptosis. Among H2S donors, isothiocyanates are endowed with a further anti-cancer mechanism: the inhibition of the histone deacetylase enzymes. On this basis, a hybrid molecule (Met-ITC) obtained through the addition of an isothiocyanate moiety to the Met molecule was designed and its ability to release Met has been demonstrated. Met-ITC exhibited more efficacy and potency than Met in inhibiting cancer cells (AsPC-1, MIA PaCa-2, MCF-7) viability and it was less effective on non-tumorigenic cells (MCF 10-A). The ability of Met-ITC to release H2S has been recorded both in cell-free and in cancer cells assays. Finally, its ability to affect the cell cycle and to induce both early and late apoptosis has been demonstrated on the most sensitive cell line (MCF-7). These results confirmed that Met-ITC is a new hybrid molecule endowed with potential anti-cancer properties derived both from Met and H2S.
Melatonin Promotes Iron Reactivation and Reutilization in Peach Plants under Iron Deficiency
Authors: Lin Lijin , Li Zhiyu , Wu Caifang , Xu Yaxin , Wang Jin , Lv Xiulan , Xia Hui , Liang Dong , Huang Zhi , Tang Yi
Published: November 2023

The yellowing of leaves due to iron deficiency is a prevalent issue in peach production. Although the capacity of exogenous melatonin (MT) to promote iron uptake in peach plants has been demonstrated, its underlying mechanism remains ambiguous. This investigation was carried out to further study the effects of exogenous MT on the iron absorption and transport mechanisms of peach (Prunus persica) plants under iron-deficient conditions through transcriptome sequencing. Under both iron-deficient and iron-supplied conditions, MT increased the content of photosynthetic pigments in peach leaves and decreased the concentrations of pectin, hemicellulose, cell wall iron, pectin iron, and hemicellulose iron in peach plants to a certain extent. These effects stemmed from the inhibitory effect of MT on the polygalacturonase (PG), cellulase (Cx), phenylalanine ammonia-lyase (PAL), and cinnamoyl-coenzyme A reductase (CCR) activities, as well as the promotional effect of MT on the cinnamic acid-4-hydroxylase (C4H) activity, facilitating the reactivation of cell wall component iron. Additionally, MT increased the ferric-chelate reductase (FCR) activity and the contents of total and active iron in various organs of peach plants under iron-deficient and iron-supplied conditions. Transcriptome analysis revealed that the differentially expressed genes (DEGs) linked to iron metabolism in MT-treated peach plants were primarily enriched in the aminoacyl-tRNA biosynthesis pathway under iron-deficient conditions. Furthermore, MT influenced the expression levels of these DEGs, regulating cell wall metabolism, lignin metabolism, and iron translocation within peach plants. Overall, the application of exogenous MT promotes the reactivation and reutilization of iron in peach plants.
CRISPR/Cas9 Mutagenesis through Introducing a Nanoparticle Complex Made of a Cationic Polymer and Nucleic Acids into Maize Protoplasts
Authors: Nagy Bettina , Öktem Ayşegül , Ferenc Györgyi , Ungor Ditta , Kalac Aladina , Kelemen-Valkony Ildikó , Fodor Elfrieda , Nagy István , Dudits Dénes , Ayaydin Ferhan
Published: November 2023

Presently, targeted gene mutagenesis attracts increasing attention both in plant research and crop improvement. In these approaches, successes are largely dependent on the efficiency of the delivery of gene editing components into plant cells. Here, we report the optimization of the cationic polymer poly(2-hydroxypropylene imine) (PHPI)-mediated delivery of plasmid DNAs, or single-stranded oligonucleotides labelled with Cyanine3 (Cy3) or 6-Carboxyfluorescein (6-FAM)-fluorescent dyes into maize protoplasts. Co-delivery of the GFP-expressing plasmid and the Cy3-conjugated oligonucleotides has resulted in the cytoplasmic and nuclear accumulation of the green fluorescent protein and a preferential nuclear localization of oligonucleotides. We show the application of nanoparticle complexes, i.e., “polyplexes” that comprise cationic polymers and nucleic acids, for CRISPR/Cas9 editing of maize cells. Knocking out the functional EGFP gene in transgenic maize protoplasts was achieved through the co-delivery of plasmids encoding components of the editing factors Cas9 (pFGC-pcoCas9) and gRNA (pZmU3-gRNA) after complexing with a cationic polymer (PHPI). Several edited microcalli were identified based on the lack of a GFP fluorescence signal. Multi-base and single-base deletions in the EGFP gene were confirmed using Sanger sequencing. The presented results support the use of the PHPI cationic polymer in plant protoplast-mediated genome editing approaches.
Structural Phase Transitions in the Double Salts (NH4)2PO3F·NH4NO3 and (NH4)2XO4·3NH4NO3 (X = Se, Cr)
Authors: Weil Matthias , Häusler Thomas , Bonneau Barbara , Füglein Ekkehard
Published: November 2023

In the context of investigating isostructural relationships between sulfates and monofluorophosphates, crystals of the double salts (NH4)2PO3F·NH4NO3 (AFP·AN) and (NH4)2XO4·3NH4NO3 (AX·3AN; X = Se, Cr) were grown from aqueous solutions and structurally characterized using X-ray diffraction and thermal analysis. Whereas the high-temperature forms of the two AX·3AN double salts are in fact isostructural with the sulfate analogue, AFP·AN crystallizes with a reduced amount of NH4NO3 and thus has a unique crystal structure. Both AFP·AN and the two AX·3AN compounds exhibit reversible structural phase transitions. Upon cooling, the monofluorophosphate double salt transforms from the monoclinic room-temperature polymorph (I; P21/n, Z = 4) to the intermediate triclinic polymorph (II; P1, Z = 4) that in turn transforms to the monoclinic low-temperature polymorph (III; P21/n, Z = 4). The two phase transitions (I) → (II) and (II) → (III) are characterized by a significant increase of the unit cell volumes upon cooling. The two AX·3AN double salts transform upon cooling from a disordered monoclinic crystal structure (P21, Z = 2) to a monoclinic polymorph with a doubled unit cell (P21/c, Z = 4). Such a phase transition is not observed for the sulfate analogue. All molecular moieties are fully ordered at −93 °C for the selenate double salt, whereas one of the nitrate anions remains disordered for the chromate double salt even at −173 °C. In all AFP·AN and AX·3AN crystal structures, the nitrate anions play a crucial role during the phase transitions, and an extensive network of N–H···O hydrogen-bonding interactions is responsible for the cohesion of the crystal.
Constraint-Aware Federated Scheduling for Data Center Workloads
Authors: Thiyyakat Meghana , Kalambur Subramaniam , Sitaram Dinkar
Published: November 2023

The use of data centers is ubiquitous, as they support multiple technologies across domains for storing, processing, and disseminating data. IoT applications utilize both cloud data centers and edge data centers based on the nature of the workload. Due to the stringent latency requirements of IoT applications, the workloads are run on hardware accelerators such as FPGAs and GPUs for faster execution. The introduction of such hardware alongside existing variations in the hardware and software configurations of the machines in the data center, increases the heterogeneity of the infrastructure. Optimal job performance necessitates the satisfaction of task placement constraints. This is accomplished through constraint-aware scheduling, where tasks are scheduled on worker nodes with appropriate machine configurations. The presence of placement constraints limits the number of suitable resources available to run a task, leading to queuing delays. As federated schedulers have gained prominence for their speed and scalability, we assess the performance of two such schedulers, Megha and Pigeon, within a constraint-aware context. We extend our previous work on Megha by comparing its performance with a constraint-aware version of the state-of-the-art federated scheduler Pigeon, PigeonC. The results of our experiments with synthetic and real-world cluster traces show that Megha reduces the 99th percentile of job response time delays by a factor of 10 when compared to PigeonC. We also describe enhancements made to Megha’s architecture to improve its scheduling efficiency.
Magnesium Strengthening in 3D Printed TCP Scaffold Composites
Authors: Escalera Carmen H. , Figueroa Ignacio Alejandro , Casas-Luna Mariano , Rodríguez-Gómez Francisco Javier , Piña-Barba Cristina , Montufar Edgar B. , Čelko Ladislav
Published: November 2023

This study reports the production of a Mg/15%β-tricalcium phosphate Ca3(PO4)2 composite by combining direct ink writing for the β-TCP preform and liquid infiltration technique to obtain a continuous metal matrix composite. The influence of the volume fraction of β-TCP and the in situ reaction between ceramic and metal on the microstructure and mechanical properties were investigated in detail. The β-TCP preform was uniformly distributed in the matrix, forming a continuous three-dimensional (3D) network. The obtained composite was characterized by means of relative density (He pycnometry), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and electron spectroscopy (EDX). The results suggested that a highly densified composite was processed. Three phases were identified as products generated by an exothermic reaction (Mg2Ca, CaO, and MgO); based on this, the chemical reaction mechanism for MgO formation was proposed. The compression and hardness tests showed that the Mg/15%β-tricalcium phosphate Ca3 (PO4)2 composite significantly improved its mechanical properties, i.e., 27% and 15% higher than pure Mg in compressive strength and yield strength, respectively. This behavior was attributed to the high densification of the resulting composite, strong chemical interfacial bonding, phase dispersion hardening (in situ phase formation), and the geometry and continuity of the reinforcement. These provided good load transfer from the Mg matrix to the reinforcement and contributed as strengthening mechanisms. The results reported in this investigation can help to design Mg/calcium phosphate continuous composites for biomedical applications.
Full Operating Range Optimization Design Method of LLC Resonant Converter in Marine DC Power Supply System
Authors: Han Xiao , Yao Xuliang , Liao Yuefeng
Published: November 2023

The marine DC power supply system is the key to a ship’s power supply, which needs to convert the energy from storage batteries or distributed power generation units into stable DC voltage for electric propulsion or the ship’s electronics. The LLC resonant converter can be used as the key power conversion link in the marine DC power supply system due to its ability to realize electrical isolation in a high-power environment and soft switching within a wide load range. Aiming at the problem of sudden changes in voltage gain at a high switching frequency under light load conditions and the problem of insufficient voltage gain under heavy load conditions due to the parasitic parameters of power devices (mainly referring to the junction capacitor), this paper first proposes a full operating range performance optimization design method. By adding an auxiliary circuit that can be opened according to the operating conditions and a multi-objective particle swarm parameter optimization method that considers the converter loss and voltage gain under heavy load conditions, the performance of the LLC resonant converter can be improved in a full range of operating conditions. Finally, the effectiveness of the proposed method is verified by an experimental prototype and compared with the conventional methods and existing solutions to highlight the superiority of the proposed method in this paper.
Evaluation of Different Gene Prediction Tools in Coccidioides immitis
Authors: Kirkland Theo N. , Beyhan Sinem , Stajich Jason E.
Published: November 2023

Gene prediction is required to obtain optimal biologically meaningful information from genomic sequences, but automated gene prediction software is imperfect. In this study, we compare the original annotation of the Coccidioides immitis RS genome (the reference strain of C. immitis) to annotations using the Funannotate and Augustus genome prediction pipelines. A total of 25% of the originally predicted genes (denoted CIMG) were not found in either the Funannotate or Augustus predictions. A comparison of Funannotate and Augustus predictions also found overlapping but not identical sets of genes. The predicted genes found only in the original annotation (referred to as CIMG-unique) were less likely to have a meaningful functional annotation and a lower number of orthologs and homologs in other fungi than all CIMG genes predicted by the original annotation. The CIMG-unique genes were also more likely to be lineage-specific and poorly expressed. In addition, the CIMG-unique genes were found in clusters and tended to be more frequently associated with transposable elements than all CIMG-predicted genes. The CIMG-unique genes were more likely to have experimentally determined transcription start sites that were further away from the originally predicted transcription start sites, and experimentally determined initial transcription was less likely to result in stable CIMG-unique transcripts. A sample of CIMG-unique genes that were relatively well expressed and differentially expressed in mycelia and spherules was inspected in a genome browser, and the structure of only about half of them was found to be supported by RNA-seq data. These data suggest that some of the CIMG-unique genes are not authentic gene predictions. Genes that were predicted only by the Funannotate pipeline were also less likely to have a meaningful functional annotation, be shorter, and express less well than all the genes predicted by Funannotate. C. immitis genes predicted by more than one annotation are more likely to have predicted functions, many orthologs and homologs, and be well expressed. Lineage-specific genes are relatively uncommon in this group. These data emphasize the importance and limitations of gene prediction software and suggest that improvements to the annotation of the C. immitis genome should be considered.
Determinants of Access to Bank Financing in SMEs in Mexico
Authors: Jiménez-Rico Artemio , Gómez-López Claudia Susana , Zamilpa Johanan
Published: November 2023

Several empirical studies indicate that the lack of financing is one of the main barriers that affects the economic growth of small and medium enterprises (SMEs). The main objective of this investigation was to determine to what extent the economic sector, the enterprise size, the characteristics inherent to the enterprise, the legal status, the variables linked to the performance of the enterprise, and the attributes of the owner influence the access to the bank financing of SMEs in Mexico. Using a discrete-response probit regression model, the impact of enterprise characteristics on the probability of obtaining a bank loan was determined. The data collected are from the Enterprise Surveys of Mexico, carried out by the World Bank. The sample of 1480 enterprises is representative by enterprise size, by economic sector, and by region. The research has a quantitative approach with a correlational scope, and a nonexperimental and transectional design. One of the main results highlights that the determinants with the greatest influence on access to bank financing are: the age, the small size, foreign participation, and the manufacturing sector. These results are consistent with other empirical studies, as well as with the pecking-order theory and the financial life-cycle theory.
A Bibliometric Analysis of Studies on Plant Endemism during the Period of 1991–2022
Authors: Wani Zishan Ahmad , Akhter Fareeda , Ridwan Qamer , Rawat Yashwant S. , Ahmad Zeeshan , Pant Shreekar
Published: November 2023

A bibliometric analysis of 349 scholarly documents published on the topic plant nativity and endemism from 1991–2022 was carried out using the ‘bibliometrix’ tool, developed through the R programming language. The results revealed a significant increase in the number of publications on plant nativity and endemism research since 1991. Of the total of 349 documents that were analyzed, 38 are single authored documents, and the average number of documents per author is 0.278. Each document has received an average of 33.67 citations, with 3.18 citations per year per document. The relationship between the number of authors and the number of articles that they have published follows a power-law distribution. Based on Bradford’s law, only eight were found to be the core sources. Mexico, followed by the USA, has produced the highest number of documents on plant nativity and endemism. The present study suggests that it is necessary to categorize data on endemism by floristic provinces, not by political subdivisions. Synecological studies are also needed, since endemic plants exist within communities. Models that accurately predict levels of endemism on the basis of easily measurable environmental variables should be useful for the rapid identification of endemic-rich areas. Recognition of the importance of plant endemism and the implementation of conservation measures is crucial for preserving biodiversity hotspots. Both in situ and ex situ conservation efforts are essential for protecting endemic species and preventing their extinction. By integrating these approaches, we can contribute to the long-term conservation and management of endemic plant species.
Cognitive Factors Affecting the Manufacturing Optimization Skills of Rural Indian BPO Workers
Authors: Vasantha Gokula , Corney Jonathan , Upadhyay Chandra Kant
Published: November 2023

Crowdsourcing offers on-demand access to large numbers of human workers to implement new forms of human–computer collaborative functionalities that can be seamlessly integrated into advanced software and algorithms. However, crowdsourcing tasks are primarily undertaken by urban rather than rural workers. To enable the development of skilled rural employment, this research aims to assess rural crowdsourcing workers’ spatial reasoning and creative abilities and their abilities to solve irregular strip packing problems associated with the manufacture of sheet materials. The study conducted experiments and data collection with 140 rural Business Processing Outsourcing (BPO) workers located in six states of India. The statistical analyses of the data collected from seven rural BPO firms (140 rural workers) reveal that rural workers can achieve a 2D packing efficiency that is up to 8% higher than that of commercial algorithm outcomes. The results suggest that rural crowdsourcing can lead to effective job creation, skill development, and, for a modest cost, it can support industries that employ CAD/CAM systems to generate geometric data for common manufacturing processes.
Terahertz Metasurfaces Exploiting the Phase Transition of Vanadium Dioxide
Authors: Liu Meng , Wei Ruxue , Taplin Jasmine , Zhang Weili
Published: November 2023

Artificially designed modulators that enable a wealth of freedom in manipulating the terahertz (THz) waves at will are an essential component in THz sources and their widespread applications. Dynamically controlled metasurfaces, being multifunctional, ultrafast, integrable, broadband, high contrasting, and scalable on the operating wavelength, are critical in developing state-of-the-art THz modulators. Recently, external stimuli-triggered THz metasurfaces integrated with functional media have been extensively explored. The vanadium dioxide (VO2)-based hybrid metasurfaces, as a unique path toward active meta-devices, feature an insulator–metal phase transition under the excitation of heat, electricity, and light, etc. During the phase transition, the optical and electrical properties of the VO2 film undergo a massive modification with either a boosted or dropped conductivity by more than four orders of magnitude. Being benefited from the phase transition effect, the electromagnetic response of the VO2-based metasufaces can be actively controlled by applying external excitation. In this review, we present recent advances in dynamically controlled THz metasurfaces exploiting the VO2 phase transition categorized according to the external stimuli. THz time-domain spectroscopy is introduced as an indispensable platform in the studies of functional VO2 films. In each type of external excitation, four design strategies are employed to realize external stimuli-triggered VO2-based THz metasurfaces, including switching the transreflective operation mode, controlling the dielectric environment of metallic microstructures, tailoring the equivalent resonant microstructures, and modifying the electromagnetic properties of the VO2 unit cells. The microstructures’ design and electromagnetic responses of the resulting active metasurfaces have been systematically demonstrated, with a particular focus on the critical role of the VO2 films in the dynamic modulation processes.
New Cyclopiane Diterpenes and Polyketide Derivatives from Marine Sediment-Derived Fungus Penicillium antarcticum KMM 4670 and Their Biological Activities
Authors: Yurchenko Anton N. , Zhuravleva Olesya I. , Khmel Olga O. , Oleynikova Galina K. , Antonov Alexandr S. , Kirichuk Natalya N. , Chausova Viktoria E. , Kalinovsky Anatoly I. , Berdyshev Dmitry V. , Kim Natalya Y. , Popov Roman S. , Chingizova Ekaterina A. , Chingizov Artur R. , Isaeva Marina P. , Yurchenko Ekaterina A.
Published: November 2023

Two new cyclopiane diterpenes and a new cladosporin precursor, together with four known related compounds, were isolated from the marine sediment-derived fungus Penicillium antarcticum KMM 4670, which was re-identified based on phylogenetic inference from ITS, BenA, CaM, and RPB2 gene regions. The absolute stereostructures of the isolated cyclopianes were determined using modified Mosher’s method and quantum chemical calculations of the ECD spectra. The isolation from the natural source of two biosynthetic precursors of cladosporin from a natural source has been reported for the first time. The antimicrobial activities of the isolated compounds against Staphylococcus aureus, Escherichia coli, and Candida albicans as well as the inhibition of staphylococcal sortase A activity were investigated. Moreover, the cytotoxicity of the compounds to mammalian cardiomyocytes H9c2 was studied. As a result, new cyclopiane diterpene 13-epi-conidiogenone F was found to be a sortase A inhibitor and a promising anti-staphylococcal agent.
Detroit Interventional Pain Assessment Scale: A Pain Score and Method for Measuring and Evaluating Post-Operative Pain Management—A Prospective Study
Authors: Boggs Lauryn J. , Stine Sasha A. , Boggs-Hughey Barbara J. , Geamanu Andreea , Little Bryan E. , Darwiche Hussein F. , Vaidya Rahul
Published: November 2023

Background and Objectives: Orthopedic surgeons commonly prescribe opioids, surpassing all medical specialties. Our objective was to develop a pain management scale that captures medication use, patient-reported pain scores, and helps orthopedic surgeons evaluate their post-operative prescribing practice. Materials and Methods: An IRB-approved prospective study followed 502 post-operative orthopedic surgery patients over a six-month period. All patients were surveyed in an orthopedic clinic at a Level 1 US Trauma Center, during a routine follow-up. Patient pain satisfaction was assessed using the validated Interventional Pain Assessment (IPA) scale, which uses three categories: 0 (no pain), 1 (tolerable pain), and 2 (intolerable pain). Daily narcotic use was translated to morphine milligram equivalents (MMEs) using the Michigan Automated Prescription System (MAPS) narcotics registry. When patient pain satisfaction and narcotic usage were combined, this scale was called the Detroit Interventional Pain Assessment (DIPA) scale. Results: The five classes based on common prescription and usage of narcotics in this cohort include the following: A (no pain medication), B (over-the-counter medication), C (occasional use of short-acting narcotics 1–30 MMEs), D (consistent/regular use of short-acting narcotics 31–79 MMEs), and E (long-duration or stronger short-acting narcotics 80+ MMEs). Patients were most satisfied with their pain management at six weeks (80.5%) and three months (75.65%), and least satisfied at two weeks (62.5%) and six months (60.9%). Additional information displayed on the DIPA graph revealed there was a significant decrease in the percentage of patients on narcotics at two weeks (65.2%) to six months (32.6%) at p < 0.001. Conclusions: The DIPA pain scale shows the relationship between patient pain perception and opioid prescription/usage, while also tracking prescriber tendencies. Providers were able to visualize their post-operative pain management progression at each designated clinic visit with corresponding alphabetical daily MME categories. In this study, results suggest that surgeons were not effective at managing the pain of patients at two weeks post-operative, which is attributed to an inadequate number of pain pills prescribed upon discharge. Overall, the DIPA graph signaled that better pain management interventions are necessitated in periods with lower efficiency scores.
A Straightforward Approach to Analyze Skeletal Muscle MRI in Limb-Girdle Muscular Dystrophy for Differential Diagnosis: A Systematic Review
Authors: Morishima Ryo , Schoser Benedikt
Published: November 2023

Skeletal muscle MRI studies in limb-girdle muscular dystrophy (LGMD) have increased over the past decades, improving the utility of MRI as a differential diagnostic tool. Nevertheless, the relative rarity of individual genotypes limits the scope of what each study can address, making it challenging to obtain a comprehensive overview of the MRI image of this splintered group. Furthermore, MRI studies have varied in their methods for assessing fat infiltration, which is essential in skeletal muscle MRI evaluation. It stayed problematic and impeded attempts to integrate multiple studies to cover the core MRI features of a distinct LGMD. In this study, we conducted a systematic review of LGMD in adults published until April 2023; 935 references were screened in PubMed and EMBASE, searches of the gray literature, and additional records were added during the screening process. Finally, 39 studies were included in our final analysis. We attempted to quantitatively synthesize the MRI data sets from the 39 individual studies. Finally, we illustrated ideal and simple MRI muscle involvement patterns of six representative LGMD genotypes. Our summary synthesis reveals a distinct distribution pattern of affected muscles by LGMD genotypes, which may be helpful for a quick first-tier differential diagnosis guiding genetic diagnostics.
Insulin Resistance: A Marker for Fat-to-Lean Body Composition in Japanese Adults
Authors: Matsui Masahiro , Fukuda Akira , Onishi Saori , Ushiro Kosuke , Nishikawa Tomohiro , Asai Akira , Kim Soo Ki , Nishikawa Hiroki
Published: November 2023

We sought to investigate the relationship between insulin resistance (IR) and body composition as assessed by bioelectrical impedance analysis in Japanese health check-up recipients (1186 men and 1441 women). IR was defined as a Homeostasis Model Assessment of IR (HOMA-IR) ≥ 2.5. In body-composition-related parameters, the fat mass index (F index) was defined as fat mass divided by the height squared (kg/m2). The fat-free mass index (FF index) was defined as fat-free mass divided by the height squared (kg/m2). The F index to FF index ratio (F-FF ratio) was defined as the F index divided by the FF index. Factors related to HOMA-IR were examined. The median HOMA-IR was 1.54 in men and 1.30 in women (p < 0.0001). The median F index was 4.9 kg/m2 in men and 6.1 kg/m2 in women (p < 0.0001). The median FF index was 18.2 kg/m2 in men and 15.1 kg/m2 in women (p < 0.0001). The median F-FF ratio was 0.272 in men and 0.405 in women (p < 0.0001). The F-FF ratio was an independent factor associated with HOMA-IR in the multivariate analysis in both genders, while the F index and FF index were not in both genders. In conclusion, fat and skeletal muscle balance can be controlled by IR in Japanese adults.
Association of Maternal Feeding Style with Fruit and Vegetable Consumption in Saudi Preschoolers: A Nationwide Cross-Sectional Study
Authors: Al-buobayd Amal Abdulaziz , Al-Otaibi Hala Hazam , Farag Hoda A. S.
Published: November 2023

Parental feeding style (PFS) remarkably influences fruit and vegetable (F&V) consumption in preschoolers. This study aimed to determine the association between PFS and preschoolers’ F&V consumption, as influenced by socioeconomic factors. A nationwide cross-sectional study was conducted among 1418 mothers of children aged 3–5 years in Saudi Arabia. Multinomial logistic regression model analysis was performed to assess the association between PFS and children’s daily F&V intake using the Parental Feeding Style Questionnaire. The influence of socioeconomic factors on this association was also evaluated. For mothers with university degrees, encouragement, emotional, and instrumental feeding enhanced their children’s intake of F&Vs, fruits, and vegetables, respectively. Children from families earning SAR >10,000 monthly had increased F&V intake with encouragement feeding and increased fruit intake with emotional feeding. However, F&V intake was reduced in children of working mothers with controlling feeding styles. Breastfeeding for <6 months was associated with increased F&V intake through emotional feeding. The most prevalent feeding style was encouragement, followed by emotional, with control and instrumental styles being less common. This study provides strong evidence on the association between PFS and daily F&V intake in Saudi preschoolers. Interventional and longitudinal studies on PFS are required to confirm these findings.
Finiteness of N=4 Super-Yang–Mills Effective Action in Terms of Dressed N=1 Superfields
Authors: Kondrashuk Igor , Schmidt Ivan
Published: November 2023

We argue in favor of the independence on any scale, ultraviolet or infrared, in kernels of the effective action expressed in terms of dressed N=1 superfields for the case of N=4 super-Yang–Mills theory. Under “scale independence” of the effective action of dressed mean superfields, we mean its “finiteness in the off-shell limit of removing all the regularizations”. This off-shell limit is scale independent because no scale remains inside these kernels after removing the regularizations. We use two types of regularization: regularization by dimensional reduction and regularization by higher derivatives in its supersymmetric form. Based on the Slavnov–Taylor identity, we show that dressed fields of matter and of vector multiplets can be introduced to express the effective action in terms of them. Kernels of the effective action expressed in terms of such dressed effective fields do not depend on the ultraviolet scale. In the case of dimensional reduction, by using the developed technique, we show how the problem of inconsistency of the dimensional reduction can be solved. Using Piguet and Sibold formalism, we indicate that the dependence on the infrared scale disappears off shell in both the regularizations.
A Retrospective Observational Study of the Impact of HIV Status on the Outcome of Paediatric Intensive Care Unit Admissions at a Tertiary Hospital in South Africa (2015–2019)
Authors: Whitehead Kim , Ballot Daynia E.
Published: November 2023

HIV-infected and HIV-exposed but uninfected (HEU) children have unique health risks. Our study looked at how HIV exposure and infection impact presentation and outcomes in PICU in an era of improved ART. A retrospective analysis of children admitted to PICU was performed. The sample was divided into HIV negative, HEU and HIV infected, and presentation and outcomes were compared with a significance level set at α = 0.05. Our study showed that 16% (109/678) of children admitted to PICU were HEU and 5.2% (35/678) were HIV infected. HIV-infected children were admitted at a younger age (median two months) with an increased incidence of lower respiratory infections than HIV-negative children (p < 0.001); they also required longer ventilation and admission (p < 0.001). HIV-infected children had a higher mortality (40%) (p = 0.02) than HIV-negative (22.7%) children; this difference was not significant when comparing only children with a non-surgical diagnosis (p = 0.273). HEU children had no significant difference in duration of ICU stay (p = 0.163), ventilation (p = 0.443) or mortality (p = 0.292) compared to HIV-negative children. In conclusion, HIV-infected children presented with more severe disease requiring longer ventilation and admission. HEU had similar outcomes to HIV-negative children.
Evaluating the Potential of Plukenetia volubilis Linneo (Sacha Inchi) in Alleviating Cardiovascular Disease Risk Factors: A Mini Review
Authors: Abd Rahman Izzat Zulhilmi , Nor Hisam Nur Syahidah , Aminuddin Amilia , Hamid Adila A. , Kumar Jaya , Ugusman Azizah
Published: November 2023

Plukenetia volubilis Linneo or Sacha Inchi (SI), a traditional natural remedy indigenous to Peru and Brazil, has garnered global attention due to its exceptional nutritional composition. Its protective effects against various non-communicable diseases, notably cardiovascular disease (CVD), have become a subject of interest in recent research. This comprehensive review summarizes the existing evidence from 15 relevant articles concerning the impact of SI on common CVD risk factors, including dyslipidemia, obesity, diabetes, and hypertension. The relevant articles were derived from comprehensive searches on PubMed, Scopus, Google Scholar, and Web of Science using predefined criteria and keywords related to the topic. Overall, SI demonstrated positive effects in attenuating dyslipidemia, obesity, diabetes, and hypertension. The multifaceted mechanisms responsible for the protective effects of SI against these CVD risk factors are primarily attributed to its antioxidative and anti-inflammatory properties. While preclinical studies dominate the current scientific literature on SI, there are limited clinical trials to corroborate these findings. Therefore, future well-designed, large-scale randomized clinical trials are highly recommended to establish the efficacy of SI and determine its optimal dosage, potential drug and food interactions, and practical integration into preventive strategies and dietary interventions for the high-risk populations.
Gathering Southward under Secularization and Syncretism: Study of the Spatial-Temporal Distribution and Influencing Factors of Chinese Historical Buddhist Architecture in Zhejiang
Authors: Ju Fei
Published: November 2023

After Buddhism spread to the Zhejiang, it underwent sinicization, giving rise to Chinese Buddhist architecture and forming a secularized character. The spatial-temporal distribution of historical Buddhist architecture in Zhejiang is strongly representative of Buddhist architecture. From the perspective of religious cultural geography, this study takes 11 prefecture-level cities of Zhejiang as the basic research units, and employs the spatial-analysis method of ArcGIS to analyze the spatial-temporal evolution characteristics of representative historical Buddhist architectural samples, and to explore the factors affecting their distribution. The research results indicate that the spatial distribution of representative historical Buddhist architectural samples in the Zhejiang is extensive, with a distinct single-core clustering characteristic. The mean-center movement of the representative historical Buddhist architectural samples in Zhejiang during different historical periods manifests in four distinct directional phases, overall moving from north to south. Natural, transportation, political, technological, economic, and religious cultural transmission factors collectively influence the spatial-temporal distribution of Buddhist architecture in Zhejiang. Especially the secularization of Buddhism and the syncretism of Confucianism, Daoism, and Buddhism have been the primary drivers in the spatial-temporal distribution evolution of Buddhist architecture.
The Impact of Meteorological Conditions and Emissions on Tropospheric Column Ozone Trends in Recent Years
Authors: Hou Xuewei , Zhang Yifan , Lv Xin , Lee James
Published: November 2023

Based on OMI/MLS data (2005–2020) and Community Earth System Model (CESM2) simulated results (2001–2020), annual variation trends of tropospheric column ozone (TCO) in the recent two decades are explored, and the separate impacts of meteorological conditions and emissions on TCO are quantified. The stratospheric ozone tracer (O3S) is used to quantify the contribution of stratospheric ozone to the trend of TCO. The evaluation shows that the simulated results capture the spatial-temporal distributions and the trends of tropospheric column ozone well. Over the East Asia and Southeast Asia regions, TCO is increasing, with a rate of ~0.2 DU/yr, which is primarily attributed to the emission changes in ozone precursors, nitrogen oxide (NOx) and volatile organic chemicals (VOCs). But the changes in meteorological conditions weaken the increase in TCO, even leading to a decrease in East Asia in spring and summer. TCO is decreasing in the middle and high latitudes of the southern hemisphere, which is mainly attributed to the changes in meteorological conditions. The increasing rates are the highest in autumn, especially over North America, East Asia, Europe and South of East Asia, with rate values of 0.20, 0.31, 0.17, and 0.32 DU/yr, respectively. Over the equatorial region, the contribution of stratospheric ozone to TCO is below 10 DU, and shows a weak positive trend of ~0.2 DU/yr. In the latitude of ~30°N/S, the stratospheric contribution is high, ~25 DU, and is affected by the sinking branch of the Brewer–Dobson circulation and stratosphere–troposphere exchange in the vicinity of tropical jet stream. The stratospheric contribution to TCO in the north of 30°N is significantly decreasing (~0.6 DU/yr) under the influence of meteorological conditions. Changes in emissions weaken the decrease in stratospheric contributions in the north of 30°N and enhance the increase in 30°S–30°N significantly. The trends of stratospheric contributions on TCO partly explain the trends of TCO which are mostly affected by the change in emissions. To control the increasing TCO, actions to reduce emissions are urgently needed.
Phenological Changes and Their Influencing Factors under the Joint Action of Water and Temperature in Northeast Asia
Authors: Wang Jia , Meng Suxin , Zhu Weihong , Xu Zhen
Published: November 2023

Phenology is an important indicator for how plants will respond to environmental changes and is closely related to biomass production. Due to global warming and the emergence of intermittent warming, vegetation in northeast Asia is undergoing drastic changes. Understanding vegetation phenology and its response to climate change is of great significance to understanding the changes in the sustainable development of ecosystems. Based on Global Inventory Modelling and Mapping Studies (GIMMS), normalized difference vegetation index (NDVI)3g data, and the mean value of phenological results extracted by five methods, combined with climatic data, this study analyzed the temporal changes in phenology and the responses to climatic factors of five vegetation types of broad-leaved, needle-leaf, mixed forests, grassland, and cultivated land in northeast Asia over 33 years (1982–2014). The results showed that, during the intermittent warming period (1999–2014), the start of the growing season (SOS) advancement (Julian days) trend of all vegetation types decreased. During 1982–2014, the average temperature sensitivity of the SOS was 1.5 d/°C. The correlation between the SOS and the pre-season temperature is significant in northeast Asia, while the correlation between the EOS and the pre-season precipitation is greater than that between temperature and radiation. The impact of radiation changes on the SOS is relatively small.