bluuebunny/crossref_metadata_2025 huggingface dataset:
Corazonin Stimulates Ecdysteroid Synthesis during the Molting Process of the Swimming Crab, Portunus trituberculatus
Authors: Xie Xi , Zhang Jun , Tu Shisheng , Zhou Qi , Zhu Dongfa
Published: August 2024

The neuropeptide corazonin (Crz) exerts diverse physiological effects in insects, yet its role in crustaceans remains elusive. The abundant expression of Crz receptor (CrzR) in the Y-organs of several crustaceans suggests a potential involvement of Crz in regulating ecdysteroid synthesis. In this study, we examined the effects of PtCrz on ecdysteroid synthesis during the molting period of Portunus trituberculatus through PtCrz treatments and PtCrzR silencing. Our results showed that PtCrz peptide stimulates ecdysteroid levels and the gene expression involved in ecdysteroidogenesis both in vitro and in vivo, whereas dsPtCrzR treatments had opposite effects on ecdysteroid levels and associated gene expression. Thus, our study suggests that PtCrz may modulate ecdysteroid synthesis via Y-organ-expressed PtCrzR. Furthermore, we also discovered the involvement of PtCrz/PtCrzR signaling in regulating PtETH expression. Notably, the inhibitory effect of dsPtCrzR on ecdysteroid synthesis or PtETH expression can be reversed by PtCrz treatment, suggesting the potential existence of multiple receptors for PtCrz. This study provides new insights into the function of crustacean Crz and, for the first time, elucidates the presence of a neuropeptide that can stimulate ecdysteroid synthesis in crustaceans.
Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma
Authors: Li Dahuan , Bao Xin , Lei Shan , Cao Wenpeng , Zeng Zhirui , Chen Tengxiang
Published: August 2024

Hepatocellular carcinoma (HCC) is a prevalent malignant digestive tumor. Numerous genetic mutations have been documented in HCC, yet the clinical significance of these mutations remains largely unexplored. The objective of this study is to ascertain the clinical value and biological effects of xin actin binding repeat containing 2 (XIRP2) mutation in HCC. The gene mutation landscape of HCC was examined using data from the Cancer Genome Atlas and the International Cancer Genome Consortium databases. The prognostic significance of the XIRP2 mutation was assessed through KM plot analysis. The association between drug sensitivity and the XIRP2 mutation was investigated using the TIDE algorithm and CCK-8 experiments. The biological effects of the XIRP2 mutation were evaluated through qRT-PCR, protein stability experiments, and relevant biological experiments. The XIRP2 mutation is one of the high-frequency mutations in HCC, and is associated with poor prognosis. A total of 72 differentially expressed genes (DEGs) were observed in HCC tissues with the XIRP2 mutation as compared to those with the XIRP2 wildtype, and these DEGs were closely related to ion metabolic processes. The XIRP2 mutation was linked to alterations in the sensitivity of fludarabine, oxaliplatin, WEHI-539, and LCL-161. CCK-8 assays demonstrated that HCC cells carrying the XIRP2 mutation exhibited increased resistance to fludarabine and oxaliplatin, but enhanced sensitivity to WEHI-539 and LCL-161 as compared with those HCC cells with the XIRP2 wildtype. The XIRP2 mutation was found to have no impact on the mRNA levels of XIRP2 in tissues and cells, but it did enhance the stability of the XIRP2 protein. Mechanically, the inhibition of XIRP2 resulted in a significant increase in sensitivity to oxaliplatin through an elevation in zinc ions and a calcium ion overload. In conclusion, the XIRP2 mutation holds potential as a biomarker for predicting the prognosis and drug sensitivity of HCC and serves as a therapeutic target to enhance the efficacy of oxaliplatin.
Comparative Analysis of Angora Rabbit Colostrum and Mature Milk Using Quantitative Proteomics
Authors: Huang Dongwei , Wang Yuanlang , Ding Haisheng , Zhao Huiling
Published: August 2024

Colostrum intake is a crucial determinant of survival in newborn rabbits. Neonates rely entirely on passive immunity transfer from their mothers while suckling colostrum. The goal of this study was to explore the protein differences of rabbit milk during different lactation periods. Our findings showed that the daily milk yield exhibited an increasing trend from the 2nd to the 21st day of lactation. A data-independent acquisition proteomics approach identified a total of 2011 proteins. Significantly, different abundances were found for 525 proteins in the colostrum and the mature milk samples. Eleven differentially abundant proteins (DAPs) were examined using parallel reaction monitoring, which verified the reliability of the proteomic data. Gene Ontology analysis revealed that these DAPs were primarily associated with glycosyltransferase activity, macromolecule transmembrane transporter activity, and regulation of acute inflammatory response. The dominant metabolic pathways of the DAPs involve the complement and coagulation cascades. A protein–protein interaction analysis identified apolipoprotein B, apolipoprotein A1, triose phosphate isomerase 1, and albumin as the hub proteins responsible for distinguishing differences between biological properties in rabbit colostrum and mature milk. These findings enhance our comprehension of the rabbit milk proteome, particularly in expanding our knowledge regarding the requirements of neonatal rabbits.
Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitors as a New Treatment Option for Anemia in Chronic Kidney Disease
Authors: Bartnicki Piotr
Published: August 2024

Anemia plays an important role in chronic kidney disease (CKD) progression because it worsens the quality of life and increases the risk of cardiovascular complications in CKD patients. In such cases, anemia is mainly caused by endogenous erythropoietin (EPO) and iron deficiencies. Therefore, KDIGO and ERBP guidelines for anemia treatment in CKD patients focus on recombinant EPO and iron supplementation. A recent new treatment option for anemia in CKD patients involves blocking the hypoxia-inducible factor (HIF) system with prolyl hydroxylase inhibitors (PHIs), what causes increasing endogenous EPO production and optimizing the use of iron. Clinical studies have shown that the hypoxia-inducible factor prolyl hydroxylase inhibitors (HIF-PHIs) covered in this manuscript—roxadustat, vadadustat, daprodustat, and molidustat—effectively increase hemoglobin (Hb) levels in both non-dialyzed and dialyzed CKD patients. Moreover, these medicines reduce blood lipid levels and do not accelerate CKD progression. However, blockage of the HIF system by HIF-PHIs may be associated with adverse effects such as cardiovascular complications, tumorogenesis, hyperkalemia. and retinopathy. More extensive and long-term clinical trials of HIF-PHIs-based anemia treatment in CKD patients are needed, and their results will indicate whether HIF-PHIs represent an effective and safe alternative to EPO and iron supplementation for anemia treatment in CKD patients.
Eucalyptus Essential Oil Inhibits Cell Infection by SARS-CoV-2 Spike Pseudotyped Lentivirus
Authors: Fernandez Sara Alonso , Pelaez-Prestel Hector F. , Ras-Carmona Alvaro , Mozas-Gutierrez Juan , Reyes-Manzanas Raquel , Reche Pedro A.
Published: August 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a public health concern due to infections with new SARS-CoV-2 variants. Therefore, finding effective preventive and therapeutic treatments against all SARS-CoV-2 variants is of great interest. In this study, we examined the capacity of eucalyptus essential oil (EEO) and eucalyptol (EOL) to prevent SARS-CoV-2 infection, using as a model SARS-CoV-2 Spike pseudotyped lentivirus (SARS-CoV-2 pseudovirus) and 293T cells transfected with human angiotensin-converting enzyme 2 (hACE2-293T cells). First, we determined the cytotoxicity of EEO and EOL using the MTT colorimetric assay, selecting non-cytotoxic concentrations ≤ 0.1% (v/v) for further analysis. Subsequently, we evaluated the capacity of EEO and EOL in cell cultures to preclude infection of hACE2-293T cells by SARS-CoV-2 pseudovirus, using a luciferase-based assay. We found that EEO and EOL significantly reduced SARS-CoV-2 pseudovirus infection, obtaining IC50 values of 0.00895% and 0.0042% (v/v), respectively. Likewise, EEO and EOL also reduced infection by vesicular stomatitis virus (VSV) pseudovirus, although higher concentrations were required. Hence, EEO and EOL may be able to inhibit SARS-CoV-2 infection, at least partially, through a Spike-independent pathway, supporting the implementation of aromatherapy with these agents as a cost-effective antiviral measure.
Association of Telomere Length in T Lymphocytes, B Lymphocytes, NK Cells and Monocytes with Different Forms of Age-Related Macular Degeneration
Authors: Khalatyan Anait S. , Shishparenok Anastasiya N. , Avetisov Konstantin S. , Gladilina Yulia A. , Blinova Varvara G. , Zhdanov Dmitry D.
Published: August 2024

Background: Age plays a primary role in the development of age-related macular degeneration (AMD). Telomere length (TL) is one of the most relevant biomarkers of aging. In our study, we aimed to determine the association of TL with T lymphocytes, B lymphocytes, NK cells or monocytes with different forms of AMD. Methods: Our study included 62 patients with AMD: geographic atrophy (GA), neovascular AMD (NVAMD) with and without macular atrophy and 22 healthy controls. Each leukocyte subtype was isolated from peripheral blood by immunomagnetic separation, and the DNA was purified. The TL in the genomic DNA was determined using qPCR by amplifying the telomere region with specific oligonucleotide primers and normalizing to the control gene. Statistical analysis was performed using R version 4.5.1. Results: We observed a statistically significant increase in TL in the T cells between the control and NVAMD groups but not for the GA group. The B cells and monocytes showed a significant decrease in TL in all AMD groups. The TL in the NK cells did not decrease in any of the AMD groups. Conclusions: The TL in the monocytes had the strongest association with AMD. It reflects a person’s “telomeric status” and may become a diagnostic hallmark of these degenerative processes.
The Current Update of Conventional and Innovative Treatment Strategies for Central Nervous System Injury
Authors: Tsai Meng-Hsuan , Wu Chi-Ying , Wu Chao-Hsin , Chen Chun-Yu
Published: August 2024

This review explores the complex challenges and advancements in the treatment of traumatic brain injury (TBI) and spinal cord injury (SCI). Traumatic injuries to the central nervous system (CNS) trigger intricate pathophysiological responses, frequently leading to profound and enduring disabilities. This article delves into the dual phases of injury—primary impacts and the subsequent secondary biochemical cascades—that worsen initial damage. Conventional treatments have traditionally prioritized immediate stabilization, surgical interventions, and supportive medical care to manage both the primary and secondary damage associated with central nervous system injuries. We explore current surgical and medical management strategies, emphasizing the crucial role of rehabilitation and the promising potential of stem cell therapies and immune modulation. Advances in stem cell therapy, gene editing, and neuroprosthetics are revolutionizing treatment approaches, providing opportunities not just for recovery but also for the regeneration of impaired neural tissues. This review aims to emphasize emerging therapeutic strategies that hold promise for enhancing outcomes and improving the quality of life for affected individuals worldwide.
Enhanced Multi-Strategy Slime Mould Algorithm for Global Optimization Problems
Authors: Dong Yuncheng , Tang Ruichen , Cai Xinyu
Published: August 2024

In order to further improve performance of the Slime Mould Algorithm, the Enhanced Multi-Strategy Slime Mould Algorithm (EMSMA) is proposed in this paper. There are three main modifications to SMA. Firstly, a leader covariance learning strategy is proposed to replace the anisotropic search operator in SMA to ensure that the agents can evolve in a better direction during the optimization process. Secondly, the best agent is further modified with an improved non-monopoly search mechanism to boost the algorithm’s exploitation and exploration capabilities. Finally, a random differential restart mechanism is developed to assist SMA in escaping from local optimality and increasing population diversity when it is stalled. The impacts of three strategies are discussed, and the performance of EMSMA is evaluated on the CEC2017 suite and CEC2022 test suite. The numerical and statistical results show that EMSMA has excellent performance on both test suites and is superior to the SMA variants such as DTSMA, ISMA, AOSMA, LSMA, ESMA, and MSMA in terms of convergence accuracy, convergence speed, and stability.
Validation of the Fatigue Impact Scale in Multiple Sclerosis Patients in Serbia
Authors: Tamaš Olivera , Kovačević Marija , Dobrodolac Aleksandra , Rašuo Bosnić Nada , Tot Šari Žužana , Despenić Livija , Vécsei László , Bencsik Krisztina , Pekmezović Tatjana , Drulović Jelena
Published: August 2024

Fatigue is one of the most frequent complaints of patients with multiple sclerosis (MS). The Fatigue Impact Scale (FIS), one of the 30 available fatigue questionnaires, is commonly applied because it evaluates multidimensional aspects of fatigue. The chief objectives of this study were to validate FIS and evaluate the psychometric properties of MS patients in Serbia. One hundred and twenty-one (121) MS patients and one hundred and twenty-two (122) age-, gender- and education-matched healthy control (HC) subjects completed the FIS and the Beck Depression Inventory. Internal consistency of the FIS subscales was determined using Cronbach’s Alpha Coefficient. Test/retest reliability with an intra-class correlation coefficient (ICC) for each FIS subscale was performed. The total FIS score and subscale scores showed statistically significant differences between the MS patients and the HC subjects in both FIS sessions. Cronbach’s Alpha was 0.966. All ICCs were statistically significant (p < 0.05). The Serbian version of this instrument may be useful as a clinical measure for fatigue and functionality in patients with MS.
Sleep, Diet, and Exercise: How Much Dementia Caregivers Are Affected?
Authors: Tsapanou Angeliki , Zoi Panagiota , Sakka Paraskeui
Published: August 2024

The current descriptive study reports the sleep, diet, and exercise patterns among 114 dementia caregivers, whose mean age was 55.7 (SD: 10.4) years, with 83 (72.8%) being women. The results indicate significant sleep dysfunction: 37.2% of caregivers reported rarely or never feeling rested upon waking, and 46.5% did not get enough sleep, with 45.6% sleeping only 5 to 5.5 h on average. Sleep latency was also prevalent, as 33.3% required 16 to 30 min to fall asleep. Dietary habits showed reliance on coffee, with 69.4% consuming it daily. Meat consumption was reported by 75%, and 60.9% ate pasta, indicating common dietary preferences. While 86.2% had one to three meals per day, 100% of the caregivers supplemented their diets with vitamins. The physical activity level was low, with 62.3% of respondents reporting no exercise in the past week. These findings underscore significant health concerns among dementia caregivers, including sleep deprivation, inadequate nutrition, and physical inactivity. The report emphasizes the need for targeted interventions to promote self-care practices that can enhance caregivers’ health, including better sleep hygiene, balanced nutrition, and regular exercise.
Improving the Sensitivity of Task-Based Multi-Echo Functional Magnetic Resonance Imaging via T2* Mapping Using Synthetic Data-Driven Deep Learning
Authors: Zhao Yinghe , Yang Qinqin , Qian Shiting , Dong Jiyang , Cai Shuhui , Chen Zhong , Cai Congbo
Published: August 2024

(1) Background: Functional magnetic resonance imaging (fMRI) utilizing multi-echo gradient echo-planar imaging (ME-GE-EPI) has demonstrated higher sensitivity and stability compared to utilizing single-echo gradient echo-planar imaging (SE-GE-EPI). The direct derivation of T2* maps from fitting multi-echo data enables accurate recording of dynamic functional changes in the brain, exhibiting higher sensitivity than echo combination maps. However, the widely employed voxel-wise log-linear fitting is susceptible to inevitable noise accumulation during image acquisition. (2) Methods: This work introduced a synthetic data-driven deep learning (SD-DL) method to obtain T2* maps for multi-echo (ME) fMRI analysis. (3) Results: The experimental results showed the efficient enhancement of the temporal signal-to-noise ratio (tSNR), improved task-based blood oxygen level-dependent (BOLD) percentage signal change, and enhanced performance in multi-echo independent component analysis (MEICA) using the proposed method. (4) Conclusion: T2* maps derived from ME-fMRI data using the proposed SD-DL method exhibit enhanced BOLD sensitivity in comparison to T2* maps derived from the LLF method.
Comparison between Single-Dose and Two-Dose Psilocybin Administration in the Treatment of Major Depression: A Systematic Review and Meta-Analysis of Current Clinical Trials
Authors: Salvetti Gianmarco , Saccenti Daniele , Moro Andrea Stefano , Lamanna Jacopo , Ferro Mattia
Published: August 2024

Current pharmacological treatments for major depressive disorder (MDD) are often only partially effective, with many patients experiencing no significant benefit, leading to treatment-resistant depression (TRD). Psilocybin, a classical serotonergic psychedelic, has emerged as a notable emerging treatment for such disorders. The aim of this systematic review and meta-analysis is to summarize and discuss the most recent evidence about the therapeutic effects of single-dose and two-dose psilocybin administration on the severity of depressive symptoms, as well as compare the efficacy of these interventions among patients with a primary diagnosis of MDD or TRD. Articles were collected from EBSCOhost and PubMed following the PRISMA guidelines, yielding 425 articles with 138 duplicates. After screening 287 records, 12 studies met the eligibility criteria and were included in the review. A quantitative analysis of the studies indicates that psilocybin is highly effective in reducing depressive symptoms severity among patients with primary MDD or TRD. Both single-dose and two-dose psilocybin treatments significantly reduced depressive symptoms severity, with two-dose administration sometimes yielding more pronounced and lasting effects. However, it is unclear if this was solely due to dosage or other factors. Future research should include standardized trials comparing these dosing strategies to better inform clinical practice.
Leveraging Artificial Intelligence to Optimize Transcranial Direct Current Stimulation for Long COVID Management: A Forward-Looking Perspective
Authors: Rudroff Thorsten , Rainio Oona , Klén Riku
Published: August 2024

Long COVID (Coronavirus disease), affecting millions globally, presents unprecedented challenges to healthcare systems due to its complex, multifaceted nature and the lack of effective treatments. This perspective review explores the potential of artificial intelligence (AI)-guided transcranial direct current stimulation (tDCS) as an innovative approach to address the urgent need for effective Long COVID management. The authors examine how AI could optimize tDCS protocols, enhance clinical trial design, and facilitate personalized treatment for the heterogeneous manifestations of Long COVID. Key areas discussed include AI-driven personalization of tDCS parameters based on individual patient characteristics and real-time symptom fluctuations, the use of machine learning for patient stratification, and the development of more sensitive outcome measures in clinical trials. This perspective addresses ethical considerations surrounding data privacy, algorithmic bias, and equitable access to AI-enhanced treatments. It also explores challenges and opportunities for implementing AI-guided tDCS across diverse healthcare settings globally. Future research directions are outlined, including the need for large-scale validation studies and investigations of long-term efficacy and safety. The authors argue that while AI-guided tDCS shows promise for addressing the complex nature of Long COVID, significant technical, ethical, and practical challenges remain. They emphasize the importance of interdisciplinary collaboration, patient-centered approaches, and a commitment to global health equity in realizing the potential of this technology. This perspective article provides a roadmap for researchers, clinicians, and policymakers involved in developing and implementing AI-guided neuromodulation therapies for Long COVID and potentially other neurological and psychiatric conditions.
After Online Innovators Receive Performance-Contingent Material Rewards: A Study Based on an Open Innovation Platform
Authors: Chu Ying , Qi Guijie , Wang Kaiping , Xu Feng
Published: August 2024

In recent years, enterprises have increasingly recognized the pivotal role of external users in driving product innovation. Open innovation platforms (OIPs), which facilitate interactions between companies and external innovators, have emerged as critical conduits in this regard. However, OIP managers face the challenge of motivating innovators to sustain their contributions. While some OIPs have implemented material incentives, the impact of such rewards on users’ ongoing innovation efforts remains uncertain. This study utilized a large-scale dataset from an OIP to examine how performance-contingent material rewards influence the subsequent behaviors of online innovators. Employing a quasi-experimental design involving propensity score matching (PSM) and difference-in-differences (DID) analysis, we found that receiving performance-contingent material rewards led to a decrease in the quantity of subsequent ideas generated by innovators. However, these rewarded innovators produced ideas of higher quality. Interestingly, the novelty of ideas submitted by innovators declined following their receiving of rewards. Moreover, newly enlisted innovators exhibited a more positive response to these incentives. Our findings provide valuable insights for platform managers seeking to optimize incentive mechanisms. We suggest adopting diversified incentive approaches and refining incentive strategies to effectively motivate continuous innovation among users on OIPs.
A Systematic Approach to Developing Sustainable Post-Disaster Shelters in the Southern Region of the United States
Authors: Khadka Alka , Yoon Soojin , Walker Richard G. , Lewis Amy King , Bae Yeonjin
Published: August 2024

This study aims to propose a sustainable shelter design involving energy savings, less environmental impact, and rapid construction. The structural design of the shelter is based on 3D-printing technology. Sustainability assessments, including life cycle analysis (LCA), life cycle energy assessment (LCEA), and energy justice of the designed shelter, were conducted to prove the sustainable shelter design. The outcomes of this study for several scenarios will not only allow decision-makers to design permanent shelters with maximized utilization of limited resources but also help local communities strengthen their ability to recover with minimal outside assistance post-disaster. Furthermore, residents can utilize the sustainable shelter to maintain critical functions, including business continuity and local business in emergencies.
Seismic Performance of Bridge Expansion Joints with and without Viscous Dampers during the 6 February 2023 Kahramanmaraş Earthquakes
Authors: Bayraktar Alemdar , Taş Yavuzhan , Akköse Mehmet , Hökelekli Emin , Ventura Carlos E. , Yang Tony Y.
Published: August 2024

Expansion joints render bridge structures highly vulnerable to damage during strong ground motions. Failures of expansion joints triggered by earthquakes not only jeopardize the post-earthquake serviceability of the bridge but also have a significant impact on the bridgeâs overall seismic performance. Despite extensive investigations and efforts to integrate these measures into design specifications aimed at mitigating the consequences of relative movements between adjacent bridge spans, major earthquakes have still revealed instances of damage related to expansion joints. On 6 February 2023, strong earthquake sequences occurred in KahramanmaraÅ, Turkey, with magnitudes of M7.7 and M7.6. The fault lines and epicenters of these shallow earthquakes were near the city and town centers and caused severe structural damage to buildings and infrastructures. There are approximately 1000 railway and highway bridges in the earthquake-affected region. Although both highway and railway bridges have generally performed well, some bridges experienced structural damage during the KahramanmaraÅ earthquakes. A large number of damage on the bridges is due to pounding and opening relative movements in expansion joints. This paper presents a comprehensive seismic evaluation of expansion joint failure mechanisms on bridges without viscous dampers during the 2023 KahramanmaraÅ earthquake sequences and an in-depth investigation into the seismic performance of bridge expansion joints equipped with viscous dampers and shock transmission unit devices are implemented utilizing the strong ground motion data collected throughout the earthquake sequences. It can be stated that the near-fault induced significant directivity and fling effects, resulting in notable velocity pulses and permanent tectonic deformations, and that these effects contributed to the failures of expansion joints, viscous damper devices, pot bearings, and shear keys.
Effect of Cyclic Soil Freezing and Thawing on the Lateral Load Response of Bridge Pile Foundations
Authors: Wang Wanping , Zhang Xiyin , Yu Shengsheng , Guan Jiada
Published: August 2024

In this article, a nonlinear static analysis model of a bridge pile foundation is established using numerical simulation, and the correctness of the model is verified via experiments. Then, the damage characteristics and mechanical behaviors of bridge pile foundations in cold regions under lateral loads are investigated based on the validated analysis model. The results showed that the impact of soil freeze–thaw cycles on the lateral performance of the pile–soil system is more pronounced in seasonally frozen regions compared with permafrost regions. Specifically, as the number of soil freeze–thaw cycles increases, there is a tendency for the lateral load capacity of the pile–soil system to decrease initially and then stabilize. It is worth noting that soil freeze–thaw cycles significantly influence both the stiffness and deformation capacity of the pile–soil system, with these parameters exhibiting a decreasing trend followed by stabilization as the number of freeze–thaw cycles increases. However, it has little effect on the shear force and bending moment of the pile foundation.
Study on the Impact of Deep Foundation Pit Construction on Nearby Elevated Structures—Case Study
Authors: Huang Junzhou , Yan Jun , Guo Kai , Yang Xingyue , Peng Sheng , Wu Cai
Published: August 2024

Urbanization and population concentration in China’s major cities drive high land utilization demands, affecting nearby bridges during underground construction. Foundation pit construction alters the internal forces, deformation, and displacement of bridge piles. To understand these impacts and assess excavation support rationality, a case study was conducted on an ultra-deep foundation pit near an elevated ring road bridge in Wuhan. Considering the engineering geological conditions of the project site, construction sequence, and traffic load on the bridge, a three-dimensional finite element model was established to simulate the impact of foundation pit excavation on the elevated structure. And through an analysis of tracked monitoring data from the construction site, comparisons were also made with the simulation results. The findings of this case study indicated that throughout the entire construction phase of the foundation pit, the maximum horizontal and vertical displacements of the bridge foundation structure caused by the construction are 2.98 mm and −1.75 mm, respectively; the maximum change rate of the bending moment in the bridge structure due to the foundation pit construction is 6.3%, while the change in the axial forces is small, and completely within the safety control standards for bridge structure displacement. By analyzing the monitoring data over three stages within one year after the completion of the foundation pit, it is shown that the bridge structure gradually tends to stabilize. Additionally, due to soil consolidation, its displacement shows uniform rebound and tends to stabilize. The research findings provide valuable reference points for the design and construction of similar deep foundation pit projects.
Design and Application of Mortars and Grouts for the Restoration of the Byzantine Church of Panaghia Krena in Chios Island, Greece
Authors: Miltiadou-Fezans Androniki , Kalagri Anna , Anagnostopoulou Sophie
Published: August 2024

The Church of Panaghia Krena is a very important Byzantine monument situated in the island of Chios, well-known due to the high aesthetic value of the ceramic tile decoration on its facades and of its frescoes. The church suffered severe damage especially due to the 1881 devastating Chios earthquake and different interventions were implemented soon after (1884), consisting mainly of the reconstruction of collapsed areas and the addition of ties. Nevertheless, deterioration of old damages and appearance of new ones was observed. Thus, a restoration program was carried out between 2000 and 2007. This paper presents the basic characteristics of all types of historic materials examined, the main results of the in situ and laboratory experimental program carried out for the selection of the raw materials, and the design of optimum compositions of mortars and grouts to be used for the restoration works. The importance of in situ pilot applications is underlined, as well as of the production of site-specific aggregates for the repointing mortars by crushing a local rock, in order to succeed the adequate reddish color to match with the original mortars and bricks still preserved and achieve an aesthetic harmony with the color hue and texture of the original materials.
Facilitating Green Transition in Small- and Medium-Sized Building Material Enterprises: Collaborative Support via Green Patent Pledge Financing Guarantees
Authors: Mei Zhu , Zhou Qiaomei , Zhang Jingjing , Mao Junjie
Published: August 2024

Purpose: This study aims to analyze the interactions within the intellectual property pledge financing framework involving SMBMEs, banks, and third-party intermediaries, focusing on factors that promote sustainable cooperation. Methods: The research employs an evolutionary game model to simulate and analyze strategic interactions among the stakeholders, considering bounded rationality and asymmetric information. Results: Key findings include the positive correlation between SMBMEs’ reputation and timely repayment, the adverse effects of patent devaluation on cooperation, and the reasonable loan interest rates for facilitating GPPFG cooperation. The study also highlights the need for a transparent information platform and reasonable breach of contract compensation. Conclusions: The paper concludes that an efficient GPPFG mechanism is crucial for SMBMEs’ financial support and green transformation. It suggests that third-party intermediaries play a significant role in risk reduction and innovation facilitation. The study acknowledges limitations and calls for future research to explore technological innovations and improve intermediary service systems for SMBMEs’ sustainable development.
Effect of Vibration Mixing on the Mechanical Properties of Carbon Nanotube-Reinforced Ultra-High-Performance Concrete
Authors: Zhou Li , Yin Jiangang , Wang Wei , Liu Fucai , Xiao Min , Yang Yibo , Cui Haibo
Published: August 2024

Vibration mixing, characterized by the high-frequency vibrations of the mixing shaft, can enhance the mechanical properties of ultra-high-performance concrete (UHPC). However, the effects of vibration mixing on carbon nanotube (CNT)-reinforced UHPC have not been previously reported. To investigate the impact of vibration mixing on the properties of CNT-reinforced UHPC, a comparative study was conducted using different vibration mixing durations and twin-shaft mixing. The results indicate that for CNT-reinforced UHPC, vibration mixing achieves better flowability, higher wet apparent density, and superior mechanical properties in shorter mixing times compared to twin-shaft mixing, making it a more favorable method. Considering vibration mixing times ranging from 3 to 7 min, the optimal time was found to be 3 min, during which the axial compressive strength increased by 3.3%, the elastic limit tensile strength and tensile strength improved by 14.6% and 15.8%, respectively, and the initial cracking strength and flexural strength increased by 12.6% and 13.4%, respectively, compared to values after 10 min of twin-shaft mixing.
Study on Dynamic Modulus Prediction Model of In-Service Asphalt Pavement
Authors: Wang Duanyi , Luo Chuanxi , Li Jian , He Jun
Published: August 2024

The dynamic modulus of in-service asphalt pavements serves as a critical parameter for the computation of residual life and the design of overlays. However, its acquisition is currently limited to laboratory dynamic modulus testing using a limited number of core samples, necessitating a reassessment of its representativeness. To facilitate the prediction of dynamic modulus design parameters through Falling Weight Deflectometer (FWD) back-calculated modulus data, an integrated approach encompassing FWD testing, modulus back-calculation, core sample dynamic modulus testing, and asphalt DSR testing was employed to concurrently acquire dynamic modulus at identical locations under varying temperatures and frequencies. Dynamic modulus prediction models for in-service asphalt pavements were developed utilizing fundamental model deduction and gene expression programming (GEP) techniques. The findings indicate that GEP exhibits superior efficacy in the development of dynamic modulus prediction models. The dynamic modulus prediction model developed can enhance both the precision and representativeness of asphalt pavement’s dynamic modulus design parameters, as well as refine the accuracy of residual life estimations for in-service asphalt pavements. Concurrently, the modulus derived from FWD back-calculation can be transmuted into the dynamic modulus adhering to a uniform standard criterion, facilitating the identification of problematic segments within the asphalt structural layer. This is of paramount importance for the maintenance or reconstruction of in-service asphalt pavements.
Glutamine Metabolism and Prostate Cancer
Authors: Erb Holger H. H. , Polishchuk Nikita , Stasyk Oleh , Kahya Uğur , Weigel Matthias M. , Dubrovska Anna
Published: August 2024

Glutamine (Gln) is a non-essential amino acid that is involved in the development and progression of several malignancies, including prostate cancer (PCa). While Gln is non-essential for non-malignant prostate epithelial cells, PCa cells become highly dependent on an exogenous source of Gln. The Gln metabolism in PCa is tightly controlled by well-described oncogenes such as MYC, AR, and mTOR. These oncogenes contribute to therapy resistance and progression to the aggressive castration-resistant PCa. Inhibition of Gln catabolism impedes PCa growth, survival, and tumor-initiating potential while sensitizing the cells to radiotherapy. Therefore, given its significant role in tumor growth, targeting Gln metabolism is a promising approach for developing new therapeutic strategies. Ongoing clinical trials evaluate the safety and efficacy of Gln catabolism inhibitors in combination with conventional and targeted therapies in patients with various solid tumors, including PCa. Further understanding of how PCa cells metabolically interact with their microenvironment will facilitate the clinical translation of Gln inhibitors and help improve therapeutic outcomes. This review focuses on the role of Gln in PCa progression and therapy resistance and provides insights into current clinical trials.
Albumin Leakage Level during Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy Is Associated with Major Complications
Authors: Kim Hyun-Chang , Han Dong Woo , Park Eun Jung , Hong Yeon Hwa , Song Young
Published: August 2024

The clinical consequences of perioperative albumin extravasation accompanying major abdominal surgery remain underexplored. We retrospectively reviewed the data of patients who underwent cytoreductive surgery (CRS) and hyperthermic intraoperative peritoneal chemotherapy (HIPEC). Parameters of albumin kinetics, including serum albumin concentration decrease (∆Alb) and extravasated albumin level (Albshift), were assessed from surgery until postoperative day (POD) 3. Logistic regression analysis identified factors associated with major complications. The association of albumin kinetics with major complications was evaluated using receiver operating characteristic (ROC) curve analysis. Serum albumin levels decreased during surgery and subsequently increased. Of the 121 analyzed patients, 25 (21%) developed major complications. The ∆Alb and Albshift during surgery and on POD 3 were greater in patients who developed major complications than in those who did not (12 ± 12 vs. 6 ± 14, p = 0.032, and 127.5 (71.9) vs. 48.5 (44.9), p < 0.001, respectively). Perioperative ∆Alb and Albshift were associated with major complications. The areas under the ROC curve of Albshift during the 3 days post-surgery and Albshift on POD 3 were 0.843 and 0.910, respectively. Albshift during the 3 days post-surgery and Albshift on POD 3 were correlated with complications (p < 0.05). In conclusion, perioperative albumin loss was associated with major complications in patients undergoing CRS and HIPEC. Albshift was associated with serious complications.
Usefulness of Tissue Biomarkers versus Prostate-Specific Membrane Antigen-Positron Emission Tomography for Prostate Cancer Biochemical Recurrence after Radical Prostatectomy
Authors: Vera Gabriela , Rojas Pablo A. , Black Joseph B. , San Francisco Ignacio F.
Published: August 2024

Despite curative-intent local therapy, approximately 27% to 53% of prostate cancer (PCa) patients experience prostate-specific antigen (PSA) recurrence, known as biochemical recurrence (BCR). BCR significantly raises the risk of PCa-related morbidity and mortality, yet there is no consensus on optimal management. Prostate-specific membrane antigen-positron emission tomography (PSMA PET) has emerged as highly sensitive imaging, distinguishing local recurrences from distant metastases, crucially influencing treatment decisions. Genomic biomarkers such as Decipher, Prolaris, and Oncotype DX contribute to refining recurrence risk profiles, guiding decisions on intensifying adjuvant therapies, like radiotherapy and androgen deprivation therapy (ADT). This review assesses PSMA PET and biomarker utility in post-radical prostatectomy BCR scenarios, highlighting their impact on clinical decision-making. Despite their promising roles, the routine integration of biomarkers is limited by availability and cost, requiring further evidence. PSMA PET remains indispensable for restaging and treatment evaluation in these patients. Integrating biomarkers and PSMA PET promises to optimize personalized management strategies for BCR, though more comprehensive consensus-building studies are needed to define their standardized utility in clinical practice.
Exploring Intermetallic Compounds: Properties and Applications in Catalysis
Authors: Hou Zhiquan , Hua Mengwei , Liu Yuxi , Deng Jiguang , Zhou Xin , Feng Ying , Li Yifan , Dai Hongxing
Published: August 2024

Intermetallic compounds (IMCs) have attracted significant attention in recent years due to their unique properties and potential applications in various fields, particularly in catalysis. This review aims to provide an in-depth understanding of IMCs, including their synthesis methods, structural characteristics, and diverse catalytic applications. The review begins with an introduction to IMCs, highlighting their distinct features and advantages over traditional catalyst materials. It then delves into the synthesis techniques employed to prepare IMCs and explores their structural properties. Subsequently, catalytic applications of the IMCs are introduced, focusing on the key reactions and highlighting their superior catalytic performance compared to conventional catalysts. Future perspectives for, and challenges to, the catalysis of IMCs are then proposed.
Catalytic Ozonation of Sulfachloropyridazine Sodium by Diatomite-Modified Fe2O3: Mechanism and Pathway
Authors: Yu Yang , Wang Lingling , Wu Zhandong , Liu Xuguo , Liu Zhen , Zhang Lijian , Li Lixin
Published: August 2024

A diatomite-modified Fe2O3 (Fe2O3/Dia) catalyst was prepared to catalyze the ozonation degradation of sulfachloropyridazine sodium (SPDZ). The chemical oxygen demand (COD) was used as the index of pollutant degradation. The catalytic ozonation experiment showed that the COD removal rate of SPDZ was 87% under Fe2O3/Dia catalysis, which was much higher than that obtained when using Fe2O3 as the catalyst. The characteristics of the Fe2O3/Dia catalyst were investigated, and the successful synthesis of the Fe2O3/Dia composite catalyst was proved by XRD, XPS, SEM, FTIR, BET and other characterization methods. The catalytic mechanism of degradation by ozone with Fe2O3/Dia was analyzed. According to free-radical trapping experiments and an in situ electron paramagnetic spectrometer characterization analysis, the main oxidizing species in the catalytic Fe2O3/Dia ozone system is ·OH. The intermediates in the degradation process of SPDZ were detected and analyzed in detail by liquid chromatography-coupled mass spectrometry. The degradation mechanism and three degradation paths of SPDZ were proposed.
The Influence of Betulin and Its Derivatives on Selected Colorectal Cancer Cell Lines’ Viability and Their Antioxidant Systems
Authors: Madej Marcel , Kruszniewska-Rajs Celina , Kimsa-Dudek Magdalena , Synowiec-Wojtarowicz Agnieszka , Chrobak Elwira , Bębenek Ewa , Boryczka Stanisław , Głuszek Stanisław , Adamska Jolanta , Kubica Sebastian , Matykiewicz Jarosław , Gola Joanna Magdalena
Published: August 2024

Oxidative stress is considered one of the main reasons for the development of colorectal cancer (CRC). Depending on the stage of the disease, variable activity of the main antioxidant enzymes, i.e., superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), is observed. Due to limited treatment methods for CRC, new substances with potential antitumor activity targeting pathways related to oxidative stress are currently being sought, with substances of natural origin, including betulin, leading the way. The betulin molecule is chemically modified to obtain new derivatives with improved pharmacokinetic properties and higher biological activity. The aim of this study was to evaluate the effects of betulin and its new derivatives on viability and major antioxidant systems in colorectal cancer cell lines. The study showed that betulin and its derivative EB5 affect the antioxidant enzyme activity to varying degrees at both the protein and mRNA levels. The SW1116 cell line is more resistant to the tested compounds than RKO, which may be due to differences in the genetic and epigenetic profiles of these lines.
Nogo Receptor Antagonist LOTUS Promotes Neurite Outgrowth through Its Interaction with Teneurin-4
Authors: Kurihara Yuji , Kawaguchi Yuki , Ohta Yuki , Kawasaki Nana , Fujita Yuki , Takei Kohtaro
Published: August 2024

Neurite outgrowth is a crucial process for organizing neuronal circuits in neuronal development and regeneration after injury. Regenerative failure in the adult mammalian central nervous system (CNS) is attributed to axonal growth inhibitors such as the Nogo protein that commonly binds to Nogo receptor-1 (NgR1). We previously reported that lateral olfactory tract usher substance (LOTUS) functions as an endogenous antagonist for NgR1 in forming neuronal circuits in the developing brain and improving axonal regeneration in the adult injured CNS. However, another molecular and cellular function of LOTUS remains unknown. In this study, we found that cultured retinal explant neurons extend their neurites on the LOTUS-coating substrate. This action was also observed in cultured retinal explant neurons derived from Ngr1-deficient mouse embryos, indicating that the promoting action of LOTUS on neurite outgrowth may be mediated by unidentified LOTUS-binding protein(s). We therefore screened the binding partner(s) of LOTUS by using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). LC-MS/MS analysis and pull-down assay showed that LOTUS interacts with Teneurin-4 (Ten-4), a cell adhesion molecule. RNAi knockdown of Ten-4 inhibited neurite outgrowth on the LOTUS substrate in retinoic acid (RA)-treated Neuro2A cells. Furthermore, a soluble form of Ten-4 attenuates the promoting action on neurite outgrowth in cultured retinal explant neurons on the LOTUS substrate. These results suggest that LOTUS promotes neurite outgrowth by interacting with Ten-4. Our findings may provide a new molecular mechanism of LOTUS to contribute to neuronal circuit formation in development and to enhance axonal regeneration after CNS injury.
Correction: Odegaard et al. Distinct Synaptic Vesicle Proteomic Signatures Associated with Pre- and Post-Natal Oxycodone-Exposure. Cells 2022, 11, 1740
Authors: Odegaard Katherine E. , Gallegos Gabriel , Koul Sneh , Schaal Victoria L. , Vellichirammal Neetha N. , Guda Chittibabu , Dutoit Andrea P. , Lisco Steven J. , Yelamanchili Sowmya V. , Pendyala Gurudutt
Published: August 2024

In the original publication [...]
Insights for Precursors Influence on the Solar-Assisted Photocatalysis of Greenly Synthesizing Zinc Oxide NPs towards Fast and Durable Wastewater Detoxification
Authors: Essawy Amr A. , Hussein Modather F. , Hasanin Tamer H. A. , El Agammy Emam F. , Alsaykhan Hissah S. , Alanazyi Rakan F. , Essawy Abd El-Naby I.
Published: August 2024

Herein, this study has examined the influence of Zn2+ sources during a biogenic-mediated pathway to synthesize ZnO nanoparticles with highly desirable solar-responsive catalytic properties. Salts of nitrate, acetate and chloride have been utilized. The ZnO powders underwent characterization using diverse analytical tools, including XRD, FTIR, Raman, BET, SEM, TEM with EDS/elemental mapping and UV-vis absorption/emission spectroscopic analyses. Accordingly, precursors have proved to affect crystallinity, morphology, surface characteristics, optical properties and the photocatalytic degradation of methylene blue (MB) model pollutant. It was observed that ZnO derived from zinc acetate precursor (Z-AC NPs) exhibits very fast photocatalytic degradation of MB at pH 11 with superior kinetic estimates of 0.314 min−1 and t1/2 = 2.2 min over many of recent reports. In contrast, the chloride precursor is not recommended along with the employed biogenic route. The intriguing findings could be directly correlated to the decreased crystal size, augmented surface area, the hexagonal morphology of the crystals, high potency in absorbing visible photons, high efficacy in separating photogenerated charge carriers and producing high amounts of •OH radicals. Further testing of Z-AC NPs in photocatalytic remediation of water samples from Dumat Aljandal Lake in Aljouf, Saudi Arabia, contaminated with MB and pyronine Y (PY) dyestuffs, showed high dye photodegradation. Therefore, this work could lead to an extremely fast avenue for decontaminating wastewater from hazmat dyestuff.