bluuebunny/crossref_metadata_2025 huggingface dataset:
Geschlechtsidentitäten im Wandel
Authors: Strittmatter Esther , Holtmann Martin
Published: April 2020

Zusammenfassung. Die Anzahl der Kinder und Jugendlichen, die sich aufgrund von Geschlechtsinkongruenzerleben und/oder Geschlechtsdysphorie in den Kliniken für Kinder- und Jugendpsychiatrie und Psychotherapie bzw. den vorhandenen Trans-Spezialsprechstunden vorstellen, ist in den letzten Jahren deutlich gestiegen. Sowohl national als auch international findet ein Diskurs über die psychotherapeutischen und medizinischen Gesundheitsbedarfe dieser jungen Menschen sowie die zugrunde liegende ethische Auslegung der Problematik statt. Die divergierenden Expertenmeinungen und fachlichen Verunsicherungen spiegeln sich nicht zuletzt in der unzureichenden psychotherapeutischen und psychiatrischen Versorgung für die geschlechtlich nonkonformen und geschlechtsdysphorischen Kinder und Jugendlichen wider. Der vorliegende Artikel gibt einen Überblick über die verfügbaren epidemiologischen Daten, den gesellschaftlichen, rechtlichen und medizinischen Paradigmenwechsel, die Entwicklungen in den neuen diagnostischen Klassifikationssysteme (DSM-5, ICD-11) sowie über wichtige Aspekte der AWMF-S3-Leitlinie „Geschlechtsinkongruenz, Geschlechtsdysphorie und Trans-Gesundheit“ für das Erwachsenenalter. Auf dieser Basis werden der aktuelle Diskurs und die ethischen Dilemmata im Kindes- und Jugendalter dargestellt sowie die sich daraus ergebenden Herausforderungen für die Kinder- und Jugendpsychiatrie und Psychotherapie skizziert.
CME-Labor 61: Neue Europäische Konsensus-Empfehlungen zur Dyslipidämie
Authors: Eckardstein Arnold von
Published: April 2020

Zusammenfassung. Der Lipidstatus dient vor allem der Abschätzung des Risikos für atherosklerotische Herz-Kreislauf-Erkrankungen (ASCVD). LDL-Cholesterin (LDL-C) ist primäres Ziel lipidsenkender Therapien. NonHDL-Cholesterin und Apolipoprotein B sind sekundäre Ziele. Die Europäischen Gesellschaften für Kardiologie und Atherosklerose haben deren therapeutischen Zielwerte für alle Risikogruppen gesenkt. Triglyzeride und HDL-Cholesterin sind ebenfalls für die Risikoschätzung empfohlen, aber keine therapeutischen Ziele. Lipoprotein(a) ist ein stark genetisch determinierter ASCVD-Risikofaktor und beinhaltet einen therapeutisch unbeeinflussbaren Teil des LDL-C. Die Qualität der Labordiagnostik aller Lipid-Risikofaktoren ist wegen zu grosser Methodenabhängigkeit und im Hinblick auf die Indikation neuer und teurer lipidmodifizierender Therapien verbesserungsbedürftig.
The Montreal Cognitive Assessment (MoCA) and Brain Structure
Authors: Sammer Gebhard , Lenz Eva
Published: April 2020

Abstract. MoCA is a short cognitive screening tool. We examined the relationship of MoCA performance to white matter integrity, gray matter volume, and surface-based measurements at normal aging in a study in which older and younger cognitively unaffected subjects participated. The sample was split according to MoCA performance, and the data were analyzed using a general linear model (Age × MoCA). We found effects in the expected direction for all methods. The main effects on age and performance as well as interactions occurred for regions associated with aging, pathological and nonpathological. Older low-performing subjects showed structural deficits compared to older high-performing subjects. Therefore, the global index of cognitive status reflects relevant features of the brain structure.
Partizipation aus der Perspektive von Kindern
Authors: Ballaschk Itala , Anders Yvonne
Published: April 2020

Zusammenfassung. Bildungsteilhabe und Partizipation gelten mittlerweile als Kernbegriffe der aktuellen bildungspolitischen Diskussion um Inklusion. Dabei meint Partizipation explizit die Möglichkeit für Kinder, aktiv an der Gestaltung ihrer Lebens- und Lernerfahrungen teilzuhaben ( Prengel, 2016 ). Der vorliegende Beitrag knüpft an dieses Konzept an und stellt Ergebnisse einer Fallstudie vor, die sich insbesondere mit der Sicht der Kinder zum Thema Partizipation und Wohlbefinden in der Kindertageseinrichtung beschäftigt. Im Rahmen des von der Europäischen Union geförderten Projektes „ISOTIS – Inclusive Education and Social Support to Tackle Inequality in Society“ wurden zehn Kinder im Alter von vier bis sechs Jahren aus einer Berliner Kindertageseinrichtung mit einem hohen Anteil an Familien mit Migrationshintergrund über Fokusgruppen und malbegleitete Gespräche dazu befragt, wie sie mit Verschiedenheit umgehen und welche Ansprüche sie selbst an eine gute Kindertagesbetreuung haben. Die Ergebnisse zeigen, welch eine bedeutende Rolle Partizipation für das Wohlbefinden der Kinder zu haben scheint. Frei zugängliche Spielmöglichkeiten und Materialien für die kreative Gestaltung, Mitbestimmung in der Gestaltung und Nutzung von Räumen sowie ein Tagesablauf, der weitestgehend individuelle Gestaltung zulässt, sind Aspekte, welche die Kinder als für sie bedeutsam beschreiben.
Detecting Flood‐Rich and Flood‐Poor Periods in Annual Peak Discharges Across Europe
Authors: Lun David , Fischer Svenja , Viglione Alberto , Blöschl Günter
Published: April 2020

This paper proposes a method from Scan statistics for identifying flood‐rich and flood‐poor periods (i.e., anomalies) in flood discharge records. Exceedances of quantiles with 2‐, 5‐, and 10‐year return periods are used to identify periods with unusually many (or few) threshold exceedances with respect to the reference condition of independent and identically distributed random variables. For the case of flood‐rich periods, multiple window lengths are used in the identification process. The method is applied to 2,201 annual flood peak series in Europe between 1960 and 2010. Results indicate evidence for the existence of flood‐rich and flood‐poor periods, as about 2 to 3 times more anomalies are detected than what would be expected by chance. The frequency of the anomalies tends to decrease with an increasing threshold return period which is consistent with previous studies, but this may be partly related to the method and the record length of about 50 years. In the northwest of Europe, the frequency of stations with flood‐rich periods tends to increase over time and the frequency of stations with flood‐poor periods tends to decrease. In the east and south of Europe, the opposite is the case. There appears to exist a turning point around 1970 when the frequencies of anomalies start to change most clearly. This turning point occurs at the same time as a turning point of the North Atlantic Oscillation index. The method is also suitable for peak‐over‐threshold series and can be generalized to higher dimensions, such as space and space‐time.
Building a Culture of Safety and Trust in Team Science
Authors: Iversen Colleen , Bolton W. , Rogers Alistair , Wilson Cathy , Wullschleger Stan
Published: April 2020

An Arctic research team of 150 members that implemented a culture of safety, inclusion, and trust as the foundation for cross-disciplinary science shares lessons from its experiences.
Protective role of endothelial calpain knockout in lipopolysaccharide-induced acute kidney injury via attenuation of the p38-iNOS pathway and NO/ROS production
Authors: Liu Zhifeng , Ji Jingjing , Zheng Dong , Su Lei , Peng Tianqing , Tang Jing
Published: April 2020

To explore the role of calpain and its signaling pathway in lipopolysaccharide (LPS)-induced acute kidney injury (AKI), animal models of endotoxemia were established by administration of LPS to mice with endothelial-specific Capn4 knockout (TEK/Capn4−/−), mice with calpastatin (an endogenous calpain inhibitor) overexpression (Tg-CAST) and mice with myeloid-specific Capn4 knockout (LYZ/Capn4−/−). Mouse pulmonary microvascular endothelial cells (PMECs) were used as a model of the microvascular endothelium and were stimulated with LPS. Renal function, renal inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS) expression, cellular apoptosis, plasma and renal levels of NO and reactive oxygen species (ROS), and phosphorylation of mitogen-activated protein kinase (MAPK) family members (p38, ERK1/2, and JNK1/2) were examined. Moreover, a calpain inhibitor, calpastatin overexpression adenoviruses and MAPK inhibitors were used. Significant renal dysfunction was induced by LPS stimulation, and recovery was observed in TEK/Capn4−/− and Tg-CAST mice but not in LYZ/Capn4−/− mice. Endothelial Capn4 knockout also abrogated the LPS-induced increases in renal iNOS expression, caspase-3 activity and apoptosis and plasma and renal NO and ROS levels but did not obviously affect renal eNOS expression. Moreover, LPS increased both calpain and caspase-3 activity, and only the expression of iNOS in PMECs was accompanied by increased phosphorylation of p38 and JNK. Inhibiting calpain activity or p38 phosphorylation alleviated the increased iNOS expression, NO/ROS production, and cellular apoptosis induced by LPS. These results suggest that endothelial calpain plays a protective role in LPS-induced AKI by inhibiting p38 phosphorylation, thus attenuating iNOS expression and further decreasing NO and ROS overproduction-induced endothelial apoptosis.
Epigenetic mechanism of SETDB1 in brain: implications for neuropsychiatric disorders
Authors: Zhu Yueyan , Sun Daijing , Jakovcevski Mira , Jiang Yan
Published: April 2020

Neuropsychiatric disorders are a collective of cerebral conditions with a multifactorial and polygenetic etiology. Dysregulation of epigenetic profiles in the brain is considered to play a critical role in the development of neuropsychiatric disorders. SET domain, bifurcate 1 (SETDB1), functioning as a histone H3K9 specific methyltransferase, is not only critically involved in transcriptional silencing and local heterochromatin formation, but also affects genome-wide neuronal epigenetic profiles and is essential for 3D genome integrity. Here, we provide a review of recent advances towards understanding the role of SETDB1 in the central nervous system during early neurodevelopment as well as in the adult brain, with a particular focus on studies that link its functions to neuropsychiatric disorders and related behavioral changes, and the exploration of novel therapeutic strategies targeting SETDB1.
The role of neutrophil death in chronic inflammation and cancer
Authors: Brostjan Christine , Oehler Rudolf
Published: April 2020

The lifespan of a neutrophil is short and limited by programmed cell death, followed by efferocytosis. When activated or exposed to insult, neutrophil death may be delayed to support neutrophil effector functions such as phagocytosis, cytokine release, and pathogen destruction by degranulation. However, neutrophils may also alter the type of cell death and thereby affect inflammatory responses and tissue remodeling. This review briefly introduces the various forms of neutrophil death including apoptosis, necrosis/necroptosis, and the formation of so-called “neutrophil extracellular traps” (NETs), and it summarizes the clearance of dead cells by efferocytosis. Importantly, distinct types of neutrophil death have been found to drive chronic inflammatory disorders and cancer. Thus, the tumor and its microenvironment can delay neutrophil apoptosis to exploit their pro-angiogenic and pro-metastatic properties. Conversely, neutrophils may enter rapid and suicidal cell death by forming extracellular traps, which are expelled DNA strands with neutrophil proteins. Components of these DNA–protein complexes such as histones, high-mobility group protein B1, or neutrophil elastase have been found to promote cancer cell proliferation, adhesion, migration, invasion, and thereby tumor metastasis. In other settings of chronic inflammatory disease such as gout, NETs have been found protective rather than detrimental, as they promoted the local degradation of pro-inflammatory cytokines by neutrophil proteases. Thus, the interaction of neutrophils with the tissue environment extends beyond the stage of the living cell and the type of neutrophil death shapes immune responses and tissue remodeling in health and disease.
Consistent RNA sequencing contamination in GTEx and other data sets
Authors: Nieuwenhuis Tim O. , Yang Stephanie Y. , Verma Rohan X. , Pillalamarri Vamsee , Arking Dan E. , Rosenberg Avi Z. , McCall Matthew N. , Halushka Marc K.
Published: April 2020

A challenge of next generation sequencing is read contamination. We use Genotype-Tissue Expression (GTEx) datasets and technical metadata along with RNA-seq datasets from other studies to understand factors that contribute to contamination. Here we report, of 48 analyzed tissues in GTEx, 26 have variant co-expression clusters of four highly expressed and pancreas-enriched genes (PRSS1, PNLIP, CLPS, and/or CELA3A). Fourteen additional highly expressed genes from other tissues also indicate contamination. Sample contamination is strongly associated with a sample being sequenced on the same day as a tissue that natively expresses those genes. Discrepant SNPs across four contaminating genes validate the contamination. Low-level contamination affects ~40% of samples and leads to numerous eQTL assignments in inappropriate tissues among these 18 genes. This type of contamination occurs widely, impacting bulk and single cell (scRNA-seq) data set analysis. In conclusion, highly expressed, tissue-enriched genes basally contaminate GTEx and other datasets impacting analyses.
Perovskite Solar Cells: A Porous Graphitic Carbon based Hole Transporter/Counter Electrode Material Extracted from an Invasive Plant Species Eichhornia Crassipes
Authors: Pitchaiya Selvakumar , Eswaramoorthy Nandhakumar , Natarajan Muthukumarasamy , Santhanam Agilan , Asokan Vijayshankar , Madurai Ramakrishnan Venkatraman , Rangasamy Balasundaraprabhu , Sundaram Senthilarasu , Ravirajan Punniamoorthy , Velauthapillai Dhayalan
Published: April 2020

Perovskite solar cells (PSCs) composed of organic polymer-based hole-transporting materials (HTMs) are considered to be an important strategy in improving the device performance, to compete with conventional solar cells. Yet the use of such expensive and unstable HTMs, together with hygroscopic perovskite structure remains a concern – an arguable aspect for the prospect of onsite photovoltaic (PV) application. Herein, we have demonstrated the sustainable fabrication of efficient and air-stable PSCs composed of an invasive plant (Eichhornia crassipes) extracted porous graphitic carbon (EC-GC) which plays a dual role as HTM/counter electrode. The changes in annealing temperature (~450 °C, ~850 °C and ~1000 °C) while extracting the EC-GC, made a significant impact on the degree of graphitization - a remarkable criterion in determining the device performance. Hence, the fabricated champion device-1c: Glass/FTO/c-TiO2/mp-TiO2/CH3NH3PbI3−xClx/EC-GC10@CH3NH3PbI3−x Clx/EC-GC10) exhibited a PCE of 8.52%. Surprisingly, the introduced EC-GC10 encapsulated perovskite interfacial layer at the perovskite/HTM interface helps in overcoming the moisture degradation of the hygroscopic perovskite layer in which the same champion device-1c evinced better air stability retaining its efficiency ~94.40% for 1000 hours. We believe that this present work on invasive plant extracted carbon playing a dual role, together as an interfacial layer may pave the way towards a reliable perovskite photovoltaic device at low-cost.
Modelling human CNS injury with human neural stem cells in 2- and 3-Dimensional cultures
Authors: Vagaska Barbora , Gillham Olivia , Ferretti Patrizia
Published: April 2020

The adult human central nervous system (CNS) has very limited regenerative capability, and injury at the cellular and molecular level cannot be studied in vivo. Modelling neural damage in human systems is crucial to identifying species-specific responses to injury and potentially neurotoxic compounds leading to development of more effective neuroprotective agents. Hence we developed human neural stem cell (hNSC) 3-dimensional (3D) cultures and tested their potential for modelling neural insults, including hypoxic-ischaemic and Ca2+-dependent injury. Standard 3D conditions for rodent cells support neuroblastoma lines used as human CNS models, but not hNSCs, but in all cases changes in culture architecture alter gene expression. Importantly, response to damage differs in 2D and 3D cultures and this is not due to reduced drug accessibility. Together, this study highlights the impact of culture cytoarchitecture on hNSC phenotype and damage response, indicating that 3D models may be better predictors of in vivo response to damage and compound toxicity.
The relationship between chronotype and intelligence: the importance of work timing
Authors: Ujma Péter P. , Baudson Tanja G. , Bódizs Róbert , Dresler Martin
Published: April 2020

Sleep-wake patterns show substantial biological determination, but they are also subject to individual choice and societal pressure. Some evidence suggests that high IQ is associated with later sleep patterns. However, it is unclear whether the relationship between IQ and later sleep is due to biological or social effects, such as the timing of working hours. We investigated the association between habitual sleep timing during work days and work-free days, working time and membership in Mensa, an organization of highly intelligent individuals (IQ  ≥130) using a sample of 1,172 adults split between Mensa members and age- and sex-matched volunteers from a large web-based database. We found no difference in chronotype, and the later sleep timing of Mensa members on work days was fully accounted for by later work start times. Our results indicate that later sleep timing in those with higher IQs is not due to physiological differences, but rather due to later work schedules. Later working times and the resulting lower social jetlag may be one of the reasons why higher IQ is associated with lower prospective morbidity and mortality.
Expectation-propagation for weak radionuclide identification at radiation portal monitors
Authors: Altmann Yoann , Di Fulvio Angela , Paff Marc G. , Clarke Shaun D. , Davies Mike E. , McLaughlin Stephen , Hero Alfred O. , Pozzi Sara A.
Published: April 2020

We propose a sparsity-promoting Bayesian algorithm capable of identifying radionuclide signatures from weak sources in the presence of a high radiation background. The proposed method is relevant to radiation identification for security applications. In such scenarios, the background typically consists of terrestrial, cosmic, and cosmogenic radiation that may cause false positive responses. We evaluate the new Bayesian approach using gamma-ray data and are able to identify weapons-grade plutonium, masked by naturally-occurring radioactive material (NORM), in a measurement time of a few seconds. We demonstrate this identification capability using organic scintillators (stilbene crystals and EJ-309 liquid scintillators), which do not provide direct, high-resolution, source spectroscopic information. Compared to the EJ-309 detector, the stilbene-based detector exhibits a lower identification error, on average, owing to its better energy resolution. Organic scintillators are used within radiation portal monitors to detect gamma rays emitted from conveyances crossing ports of entry. The described method is therefore applicable to radiation portal monitors deployed in the field and could improve their threat discrimination capability by minimizing “nuisance” alarms produced either by NORM-bearing materials found in shipped cargoes, such as ceramics and fertilizers, or radionuclides in recently treated nuclear medicine patients.
A synchronized VUV light source based on high-order harmonic generation at FLASH
Authors: Appi Elisa , Papadopoulou Christina C. , Mapa Jose Louise , Wesavkar Nishad , Jusko Christoph , Mosel Philip , Ališauskas Skirmantas , Lang Tino , Heyl Christoph M. , Manschwetus Bastian , Brachmanski Maciej , Braune Markus , Lindenblatt Hannes , Trost Florian , Meister Severin , Schoch Patrizia , Treusch Rolf , Moshammer Robert , Hartl Ingmar , Morgner Uwe , Kovacev Milutin
Published: April 2020

Ultrafast measurements in the extreme ultraviolet (XUV) spectral region targeting femtosecond timescales rely until today on two complementary XUV laser sources: free electron lasers (FELs) and high-harmonic generation (HHG) based sources. The combination of these two source types was until recently not realized. The complementary properties of both sources including broad bandwidth, high pulse energy, narrowband tunability and femtosecond timing, open new opportunities for two-color pump-probe studies. Here we show first results from the commissioning of a high-harmonic beamline that is fully synchronized with the free-electron laser FLASH, installed at beamline FL26 with permanent end-station including a reaction microscope (REMI). An optical parametric amplifier synchronized with the FEL burst mode drives the HHG process. First commissioning tests including electron momentum measurements using REMI, demonstrate long-term stability of the HHG source over more than 14 hours. This realization of the combination of these light sources will open new opportunities for time-resolved studies targeting different science cases including core-level ionization dynamics or the electron dynamics during the transformation of a molecule within a chemical reaction probed on femtosecond timescales in the ultraviolet to soft X-ray spectral region.
Animal bioturbation preserved in Pleistocene magadiite at Lake Magadi, Kenya Rift Valley, and its implications for the depositional environment of bedded magadiite
Authors: Buatois Luis A. , Renaut Robin W. , Owen Richard Bernhart , Behrensmeyer Anna K. , Scott Jennifer J.
Published: April 2020

Magadiite, a rare hydrous sodium-silicate mineral [NaSi7O13(OH)3·4(H2O)], was discovered about 50 years ago in sediments around Lake Magadi, a hypersaline alkaline lake fed by hot springs in the semi-arid southern Kenya Rift Valley. Today this harsh lacustrine environment excludes most organisms except microbial extremophiles, a few invertebrates (mostly insects), highly adapted fish (Alcolapia sp.), and birds including flamingos. Burrows discovered in outcrops of the High Magadi Beds (~25–9 ka) that predate the modern saline (trona) pan show that beetles and other invertebrates inhabit this extreme environment when conditions become more favourable. Burrows (cm-scale) preserved in magadiite in the High Magadi Beds are filled with mud, silt and sand from overlying sediments. Their stratigraphic context reveals upward-shallowing cycles from mud to interlaminated mud-magadiite to magadiite in dm-scale units. The burrows were formed when the lake floor became fresher and oxygenated, after a period when magadiite precipitated in shallow saline waters. The burrows, probably produced by beetles, show that trace fossils can provide evidence for short-term (possibly years to decades) changes in the contemporary environment that might not otherwise be recognised or preserved physically or chemically in the sediment record.
Assigning a role for chemosensory signal transduction in Campylobacter jejuni biofilms using a combined omics approach
Authors: Tram Greg , Klare William P. , Cain Joel A. , Mourad Basem , Cordwell Stuart J. , Day Christopher J. , Korolik Victoria
Published: April 2020

Biofilms of the gastroenteric pathogen C. jejuni may serve an important role in the transmission of infection from reservoirs of infection to humans. Herein, we undertook a combinatorial approach examining differential gene expression and protein abundance during biofilm formation in C. jejuni. Biofilms induced a substantial rearrangement of the C. jejuni transcriptome and proteome, with ~600 genes differentially expressed when compared to planktonic cells. Genes and proteins induced in biofilms were involved in iron metabolism and acquisition, cell division, glycan production and attachment, while those repressed were associated with metabolism, amino acid usage, and large tracts of the chemotaxis pathway. We further examined the role of chemotaxis in C. jejuni biofilm formation by examining isogenic strains with deletions of the cheV and cheW signal transduction genes. Both ∆cheV and ∆cheW exhibited a significant decrease in directed motility when compared to wild-type C. jejuni as well as demonstrating an increase in autoagglutination ability and biofilm formation. A subtle difference was also observed between the phenotypes of ∆cheV and ∆cheW mutants, both in motility and biofilm formation. This suggests roles for CheV and CheW and may present signal transduction as a potential method for modulating C. jejuni biofilm formation.
A high-resolution wind damage model for Europe
Authors: Koks E. E. , Haer. T.
Published: April 2020

Extreme wind events are among the costliest natural disasters in Europe, causing severe damages every year. Despite the significant impact, damages related to windstorms are an understudied topic in academia. For damage estimates, the community mostly relies on post-disaster insurance data, which is often not publicly available. Few studies offer more generic tools, but again these are often based on non-disclosed insurance data. To offer a generic, high-resolution, reproducible, and publicly accessible tool, this study presents a wind damage model that is built around publicly available hazard, exposure, and vulnerability data. We apply the model to assess building damages related to extratropical storms in Europe, but the methodology is applicable globally, given data availability, and to other hazards for which similar risk frameworks can be applied. The results show that for Europe, coastal regions are affected the most, with the United Kingdom, Ireland, Germany, France, the Netherlands, and Denmark as most affected countries. We find that the modelled damage estimates are in line with reported damages for a series of historical storms. The model is distributed as an open-source model to offer a transparent and useable windstorm damage model to a broad audience.
Development of new genetic resources for faba bean (Vicia faba L.) breeding through the discovery of gene-based SNP markers and the construction of a high-density consensus map
Authors: Carrillo-Perdomo E. , Vidal A. , Kreplak J. , Duborjal H. , Leveugle M. , Duarte J. , Desmetz C. , Deulvot C. , Raffiot B. , Marget P. , Tayeh N. , Pichon J. P. , Falque M. , Martin O. C. , Burstin J. , Aubert G.
Published: April 2020

Faba bean (Vicia faba L.) is a pulse crop of high nutritional value and high importance for sustainable agriculture and soil protection. With the objective of identifying gene-based SNPs, transcriptome sequencing was performed in order to reduce faba bean genome complexity. A set of 1,819 gene-based SNP markers polymorphic in three recombinant line populations was selected to enable the construction of a high-density consensus genetic map encompassing 1,728 markers well distributed in six linkage groups and spanning 1,547.71 cM with an average inter-marker distance of 0.89 cM. Orthology-based comparison of the faba bean consensus map with legume genome assemblies highlighted synteny patterns that partly reflected the phylogenetic relationships among species. Solid blocks of macrosynteny were observed between faba bean and the most closely-related sequenced legume species such as pea, barrel medic or chickpea. Numerous blocks could also be identified in more divergent species such as common bean or cowpea. The genetic tools developed in this work can be used in association mapping, genetic diversity, linkage disequilibrium or comparative genomics and provide a backbone for map-based cloning. This will make the identification of candidate genes of interest more efficient and will accelerate marker-assisted selection (MAS) and genomic-assisted breeding (GAB) in faba bean.
CRISPR-mediated gene targeting of CK1δ/ε leads to enhanced understanding of their role in endocytosis via phosphoregulation of GAPVD1
Authors: Guillen Rodrigo X. , Beckley Janel R. , Chen Jun-Song , Gould Kathleen L.
Published: April 2020

Human casein kinase 1 delta (CK1δ) and epsilon (CK1ε) are members of a conserved family of abundant, ubiquitously expressed serine/threonine kinases that regulate multiple cellular processes including circadian rhythm and endocytosis. Here, we have investigated the localization and interactomes of endogenously tagged CK1δ and CK1ε during interphase and mitosis. CK1δ and CK1ε localize to centrosomes throughout the cell cycle, and in interphase cells to the nucleus, and in both a diffuse and punctate pattern in the cytoplasm. Also, for the first time, they were detected at the midbody during cell division. Mass spectrometry analysis identified a total of 181 proteins co-purifying with a Venus multifunctional (VM)-tagged CK1δ and/or CK1ε. GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1), a protein required for efficient endocytosis, was consistently one of the most abundant interacting partners. We demonstrate that GAPVD1 is a substrate of CK1δ/ε with up to 38 phosphorylated residues in vitro and in vivo. Wildtype and a phosphomimetic mutant of GAPVD1, but not a phospho-ablating mutant, were able to rescue defects in transferrin and EGF internalization caused by loss of endogenous GAPVD1. Our results indicate that GAPVD1 is an important interacting partner and substrate of CK1δ/ε for endocytosis.
Plasma Donors in the Southwestern United States Positively Contribute to the Diverse Therapeutic Antibody Profile of Immune Globulin Products
Authors: Ciencewicki Jonathan M. , Schouest Katherine R. , Gierman Todd M. , Vandeberg Peter J. , Gooch Barry D.
Published: April 2020

Human-plasma-derived immune globulin (IG) is used in augmentation therapy to provide protective levels of antibodies to patients with primary immune deficiency diseases (PIDD) and for prophylaxis against infectious diseases. To maintain the breadth of antibodies necessary for clinical protection, it is important to understand regional patterns of antibody seroprevalence in source plasma from which IG products are manufactured. In this study, source plasma from donation centers in various locations of the Southwestern quarter of the United States was surveyed for antibody titers to hepatitis A virus (HAV), measles virus (MeV), and cytomegalovirus (CMV). A broad range of anti-HAV Ig plasma titers was observed among these centers, with some centers exhibiting 3–5 times the titers of the others. Minor to no differences were observed for levels of anti-MeV and anti-CMV, respectively. Importantly, elevated anti-HAV Ig titers were broadly observed across plasma units obtained from the centers exhibiting high titers, indicative of a potential regional phenomenon among donors as opposed to few donors with singularly high titers. Plasma from these high-titer centers conferred significantly greater neutralization against HAV in vitro. The outcomes of this study give a glimpse of the antibody diversity inherent in human plasma used to manufacture IG products..
Effects of visual information presented by augmented reality on children’s behavior
Authors: Shirai Nobu , Kondo Lisa , Imura Tomoko
Published: April 2020

The effects on children’s behavior of visual information presented by augmented reality (AR) were investigated. A human-like AR character was presented standing in one of two physical pathways to children aged 5–7 years old and 8–10 years old before they completed a filler task. After the task, the children were required to walk through one of the two pathways to obtain a reward. Both the 5–7- and 8–10-year-olds chose the pathway that was not associated with the AR character more frequently than the pathway that was. Subsequently, adult participants tested in a similar manner showed no significant bias in pathway selection. Taken together, these results suggest that the presentation of an AR character within the present experimental setting affected the behavior of children aged from 5–10 years but not that of adults. The results are discussed in the context of developmental changes in sensitivity to insubstantial agents (e.g., imaginary companion), the reality of information displayed by AR technology, and differences in the methods of AR presentation (e.g., hand-held devices vs. head-mounted devices).
Continuous Hypodynamic Change of Cerebrospinal Fluid Flow as A Potential Factor Working for Experimental Scoliotic Formation
Authors: Zhao Zhi , Li Tao , Bi Ni , Shi Zhiyue , Zhang Ying , Li Quan , Wang Yingsong , Xie Jingming
Published: April 2020

Scoliosis is often associated with syringomyelia (SM). As an important role in SM formation, the influence from abnormal cerebrospinal fluid (CSF) flow is still unclear to scoliosis. The aim of this experimental work is to explore the connection between CSF flow and scoliosis through imaging and histological analysis on the basis of a kaolin-induced scoliotic rabbit model. For imaging observation, in 40 kaolin-induced rabbits by C7 spinal cord injection, through pre- and postoperative MRI and radiography, CSF flow and scoliosis formation were detected at consecutive phases. According to the final formation of scoliosis until postoperative week 12, the kaolin-induced rabbits were divided into 2 groups. Through comparing the 2 groups, the relationship between the changes of CSF flow velocity and scoliosis formation were reviewed and analyzed. For histological observation, another 20 kaolin-induced rabbits were used for consecutive histological observations of spinal cord at postoperative 3-day, 2-week, 4-week and 6-week. After kaolin-induction, abnormal spinal coronal curve was observed from postoperative week 6 in the 37 survived rabbits. At postoperative week 12, scoliosis formation was detected in 73.0% kaolin-induced rabbits and the mean Cobb angle was 27.4°. From the comparison between scoliotic and non-scoliotic groups, the difference of the velocities of CSF flow was more obviously from postoperative week 4 to 12, especially after week 6. In the scoliotic group, the peak velocity of CSF flow was diseased gradually following scoliosis formation after induction. Moreover, the decrease of the peak velocities of CSF flow from preoperation to postoperative 12 weeks (ΔVmax), including up-flow (ΔVUmax) and down-flow (ΔVDmax), were positively correlated to the final scoliotic Cobb angle (P < 0.01). Through histological observation at different phases, the distinctive pathological changes of the spinal cord included early inflammatory reaction, adhesion and blockage in the subarachnoid space and the central canal, perivascular space enlargement, central canal expansion, which suggested the CSF flow being blocked by multiple ways after kaolin-induction. In conclusion, experimental scoliosis can be successfully induced by intraspinal kaolin injection. In this model, continuous hypodynamic change of CSF flow was correlated to the formation of scoliosis, which could be an important factor of scoliotic pathogenesis being explored furtherly.
Deficiency of CD44 prevents thoracic aortic dissection in a murine model
Authors: Hatipoglu Omer F. , Miyoshi Toru , Yonezawa Tomoko , Kondo Megumi , Amioka Naofumi , Yoshida Masashi , Akagi Satoshi , Nakamura Kazufumi , Hirohata Satoshi , Ito Hiroshi
Published: April 2020

Thoracic aortic dissection (TAD) is a life-threatening vascular disease. We showed that CD44, a widely distributed cell surface adhesion molecule, has an important role in inflammation. In this study, we examined the role of CD44 in the development of TAD. TAD was induced by the continuous infusion of β-aminopropionitrile (BAPN), a lysyl oxidase inhibitor, and angiotensin II (AngII) for 7 days in wild type (WT) mice and CD44 deficient (CD44-/-) mice. The incidence of TAD in CD44-/- mice was significantly reduced compared with WT mice (44% and 6%, p < 0.01). Next, to evaluate the initial changes, aortic tissues at 24 hours after BAPN/AngII infusion were examined. Neutrophil accumulation into thoracic aortic adventitia in CD44-/- mice was significantly decreased compared with that in WT mice (5.7 ± 0.3% and 1.6 ± 0.4%, p < 0.01). In addition, BAPN/AngII induced interleukin-6, interleukin-1β, matrix metalloproteinase-2 and matrix metalloproteinase-9 in WT mice, all of which were significantly reduced in CD44−/− mice (all p < 0.01). In vitro transmigration of neutrophils from CD44−/− mice through an endothelial monolayer was significantly decreased by 18% compared with WT mice (p < 0.01). Our findings indicate that CD44 has a critical role in TAD development in association with neutrophil infiltration into adventitia.
Proteomic analysis of knee cartilage reveals potential signaling pathways in pathological mechanism of Kashin-Beck disease compared with osteoarthritis
Authors: Lei Jian , Amhare Abebe Feyissa , Wang Liyun , Lv Yizhen , Deng Huan , Gao Hang , Guo Xiong , Han Jing , Lammi Mikko J.
Published: April 2020

The pathological mechanism of Kashin-Beck disease (KBD), an endemic osteoarthritic disease, remains to be poorly understood. This study was designed to identify signaling pathways and crucial proteins involved in the pathological mechanism of KBD compared with osteoarthritis (OA). The knee cartilage samples were collected from gender- and age-matched KBD (n = 9) and OA (n = 9) patients. After pre-processing, samples were labeled with Tamdem Mass Tags 6plex multiplex kit, and analyzed by liquid chromatography-tandem mass spectrometry. Proteomic results were analyzed with gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interactions (PPI). The differential abundance proteins from KBD and OA were validated using western blot analysis. As a result, A total number of 375 proteins were identified to have differential abundance between KBD and OA, of which 121 and 254 proteins were observed to be up-regulated or down-regulated in KBD group. GO analysis shows that the differential abundant proteins are associated with cell junction and signal transducer activity from extracellular to intracellular. KEGG pathways enrichment and PPI network indicate four major pathways, including extracellular matrix -receptor interaction, focal adhesion, phosphatidylinositol 3-kinase (PI3K)-Protein kinase B (Akt), and Ras signaling pathways were involved in the degeneration of cartilage. Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. In conclusion, our results demonstrated that compared with OA, the differential abundance proteins and signaling pathways may contribute to the occurrence and development of joint damage in KBD. Further investigation of their regulative roles and interaction may provide new insights into the pathological mechanisms and therapeutic targets for KBD.
Pterostilbene prevents LPS-induced early pulmonary fibrosis by suppressing oxidative stress, inflammation and apoptosis in vivo
Authors: Yang Huahong , Hua Cong , Yang Xiaolin , Fan Xiaoye , Song Hongyu , Peng Liping , Ci Xinxin
Published: April 2020

Schematic model of the protective effects by Pts on LPS-induced lung fibrosis.
Exploring the mechanism of the Fe(iii)-activated Fenton-like reaction based on a quantitative study
Authors: Qin Hehe , Fan Jinhong , Mao Shun
Published: April 2020

The Fenton method is an important water treatment process.
Nitroaromatics as n-type organic semiconductors for field effect transistors
Authors: Niazi Muhammad Rizwan , Hamzehpoor Ehsan , Ghamari Pegah , Perepichka Igor F. , Perepichka Dmitrii F.
Published: April 2020

The utility of the NO2-group in the design of organic semiconductors is demonstrated by fabricating OFETs with 5 nitrofluorenone derivatives and analyzing the effects of molecular and crystal structure on their charge transport characteristics.
Enhancing the catalytic properties of well-defined electrophilic platinum complexes
Authors: Ríos Pablo , Rodríguez Amor , Conejero Salvador
Published: April 2020

Well-defined electrophilic or low-electron count platinum complexes are very versatile catalysts for a variety of chemical transformations.
Improving the structural and cyclic stabilities of P2-type Na0.67MnO2 cathode material via Cu and Ti co-substitution for sodium ion batteries
Authors: Zhang Xueping , Qiu Feilong , Jiang Kezhu , He Ping , Han Min , Guo Shaohua , Zhou Haoshen
Published: April 2020

An air-stable Na0.67Mn0.7Cu0.15Ti0.15O2 (NMCT) has been synthesized using a solid-state method. And it showed the excellent performance as a cathode material for sodium ion batteries.
Catalytic cycle of carbohydrate dehydration by Lewis acids: structures and rates from synergism of conventional and DNP NMR
Authors: Jensen Pernille Rose , Meier Sebastian
Published: April 2020

Structures and rates in the catalytic cycle of carbohydrate dehydration by Lewis acidic salt are determined through the systematic use of complementary NMR approaches.
Interface hybridization and spin filter effect in metal-free phthalocyanine spin valves
Authors: Zhang Xianmin , Tong Junwei , Ruan Liuxia , Yao Xiannian , Zhou Lianqun , Tian Fubo , Qin Gaowu
Published: April 2020

Spin–orbit coupling has been regarded as the core interaction to determine the efficiency of spin conserved transport in semiconductor spintronics. Here, we show the spin filter effect should be responsible for the magnetoresistance of H2Pc device.
Impact of onions in tomato-based sauces on isomerization and bioaccessibility of colorless carotenes: phytoene and phytofluene
Authors: Yu Jiahao , Gleize Béatrice , Zhang Lianfu , Caris-Veyrat Catherine , Renard Catherine M. G. C.
Published: April 2020

The addition of onions into tomato purees favored E/Z-isomerization of PT and PTF during heating tomato-based sauces.
Enantiomeric copper based anticancer agents promoting sequence-selective cleavage of G-quadruplex telomeric DNA and non-random cleavage of plasmid DNA
Authors: Parveen Sabiha , Cowan J A , Yu Zhen , Arjmand Farukh
Published: April 2020

Copper-based binuclear enantiomeric complexes 1S and 1R were synthesized as anticancer chemotherapeutic agents to target G-quadruplex rich region of DNA and thoroughly characterized by various spectroscopic and single X-ray crystal diffraction studies. The structure elucidation of Schiff base ligand LS and complexes 1S & 1R, was carried out by single crystal X-ray studies which showed that ligand crystallized in the monoclinic P21/n space group while complexes 1S and 1R crystallized in triclinic space groups P1̄ and P1, respectively with two copper units connected to each other via an alkoxide bridge to exhibit square planar geometry which is in good agreement with other spectroscopic studies {IR, ESI-MS, EPR and magnetic moment values}. In vitro binding studies of complexes 1S and 1R were carried out with G-quadruplex DNA and CT-DNA which showed higher binding affinity and selectivity toward quadruplex DNA over the duplex DNA. To validate the potential of complexes to act as therapeutic drug candidates, the cleavage studies of complexes 1S and 1R were carried out with G-quadruplex telomeric DNA by PAGE Gel assay which showed sequence selective cleavage of 22G4 via oxidative cleavage pathway. The major cleavage sites identified were G15, T6, G8, G9, G14 for complex 1S whereas for 1R G15, G20, G21, G14 cleavage sites were observed. Furthermore, these complexes were capable of cleaving pUC19 plasmid DNA in double-stranded non-random fashion which is considered to be more potent than single-strand cleavage as a source of lethal DNA lesions. Cellular studies of 1S and 1R were performed on a panel of human cancer cell lines; Huh7, MCF7, BxPC3 and AsPC1, which displayed significant cytotoxicity and differential responses toward different cancer phenotypes.
Ammonolysis synthesis of nickel molybdenum nitride nanostructures for high-performance asymmetric supercapacitors
Authors: Kumar Rudra , Bhuvana Thiruvelu , Sharma Ashutosh
Published: April 2020

Nickel molybdenum nitride nanorods were synthesised by the hydrothermal method followed by calcination and ammonolysis and tested as an electrode material for asymmetric supercapacitors.
Access to cyano-substituted pyrazolines through copper-catalyzed cascade cyanation/cyclization of unactivated olefins
Authors: Meng Fei , Fang Qin , Yuan Weidong , Xu Ning , Cao Shujun , Chun Jianlin , Li Jie , Zhang Honglin , Zhu Yingguang
Published: April 2020

A mild copper-catalyzed cascade cyanation/cyclization of hydrazone-tethered unactivated olefins was developed for the efficient and practical synthesis of cyano-containing pyrazolines.
Cationic indium catalysts for ring opening polymerization: tuning reactivity with hemilabile ligands
Authors: Goonesinghe Chatura , Roshandel Hootan , Diaz Carlos , Jung Hyuk-Joon , Nyamayaro Kudzanai , Ezhova Maria , Mehrkhodavandi Parisa
Published: April 2020

This is an in-depth study into the role hemilabile ligands play in tuning stability and reactivity in cationic indium complexes.
Carbon nanosheets from biomass waste: insights into the role of a controlled pore structure for energy storage
Authors: Zhang Limin , Zhao Wenqing , Jiang Feng , Tian Mengjie , Yang Yue , Ge Peng , Sun Wei , Ji Xiaobo
Published: April 2020

The reversible capacity and surface-controlled contributions in porous carbon nanosheet materials were explored.
The highly efficient collection of underwater oil droplets on an anisotropic porous cone surface via an electric field
Authors: Yan Yufeng , Zhang Qiuya , Li Yan , Guo Zhenyan , Tian Dongliang , Zhang Xiaofang , Jiang Lei
Published: April 2020

The highly efficient collection of underwater oil droplets on an anisotropic porous cone surface via an electric field was demonstrated.
A Survey of Counseling Curricula Among Accredited Communication Sciences and Disorders Graduate Student Programs
Authors: Doud Aspen K. , Hoepner Jerry K. , Holland Audrey L.
Published: April 2020

Purpose

The purpose of this article is to examine the current state of counseling curriculum within the discipline. The last systematic survey of counseling curriculum within the disciplines of communication sciences and disorders was completed with data from 1983 (McCarthy et al., 1986). The Council on Academic Accreditation in Audiology and Speech-Language Pathology (2017) states that counseling should be included in accredited programs but does not specify to what extent. Currently, there are no standards to specify number of credits, need for a stand-alone course, or guidance regarding content delivered.

Method

The present investigation collected data on the status of counseling curricula in accredited communication sciences and disorders graduate programs. A Qualtrics survey was distributed to identify counseling curriculum practices across accredited programs. Quantitative data such as percentages and frequency counts were compiled to summarize program offerings. Qualitative analyses were used to characterize written responses. Survey responses were also cross-validated with a review of offerings listed on program websites.

Results

Of programs currently accredited by the Council on Academic Accreditation in Audiology and Speech-Language Pathology, 42.4% responded to the current survey. Fifty-nine percent of programs offer a stand-alone course. Review of curricula from program websites indicated that only 40% of accredited programs offer a stand-alone counseling course. Quantitative details about requirements, number of credits, and embedding counseling within other courses were compared to data from the 1983 survey. Qualitative analyses identified common learner outcomes and the nature of course or curricular content.

Conclusions

Investigators found a lack of consistency in incorporating counseling across programs and discussed implications of this in speech-language pathology practice. A decrease in the number of programs that offer a stand-alone counseling course was identified as compared to offerings in 1983, as well as a disparity regarding how programs provide training in counseling. Furthermore, survey responses differed from curriculum listings on program websites. Information derived from this study may serve as a starting point for the development of flexible standards that provide direction for achieving consistent preparation of counseling skills.

Supplemental Material

https://doi.org/10.23641/asha.12149703