bluuebunny/crossref_metadata_2025 huggingface dataset:
Protective Effects of Chlorella vulgaris Supplemented Diet on Antibacterial Activity and Immune Responses in Rohu Fingerlings, Labeo rohita (Hamilton), Subjected to Aeromonas hydrophila Infection
Authors: Pradhan Jyotirmayee , Sahu Swagatika , Das Basanta
Published: April 2023

The current study focuses on the antibacterial activity and potential efficiency of dietary supplements of Chlorella vulgaris on the immune response, improved growth performance, and disease resistance of Labeo rohita fingerlings against Aeromonas hydrophila infection. Crude ethanolic extract of Chlorella and partially purified fractions of the extract were tested against two selected fish pathogens using the disc diffusion method. A total number of 360 rohu fingerlings (25 ± 2 g) were allocated to 4 treatments for 90 days. They were fed with an experimental diet containing Chlorella powder (0, 0.1, 0.5, and 1.0 g Kg−1 of a basic diet). To evaluate the non-specific immunity parameters including serum bactericidal, lysozyme activity, superoxide anion production, and biochemical and haematological indices, the fish were sampled at day 30, 60, 90, and after bacterial challenge. Mortalities of the fish were observed over 10 days post challenge with A. hydrophila. The protein and globulin levels of the treatment group were significantly higher after being treated with Chlorella than those of the control group. The total blood-cell count and haemoglobin content were also increased in the algal-diet-treated group. Among all the experimental diets, the 0.5 g Kg−1Chlorella fed group of fish showed significantly (p < 0.05) increased serum bactericidal activity and superoxide anion production when compared with the control group on day 90. Maximum lysozyme activity (750.00 ± 3.27) was noticed in the 1.0 g Kg−1 diet fed group on day 30. The Chlorella treated group exhibited a better growth performance of the fish. The maximum survivability (86.5%) was recorded in the 1.0 g Kg−1 diet fed group at the end of the 10-day fish exposure to A. hydrophila. These results suggest that the optimum dietary Chlorella supplementation could be 0.5–1.0 g Kg−1 of the diet, which stimulates immunity and protects L. rohita from A. hydrophila infection.
Deep Learning for Echocardiography: Introduction for Clinicians and Future Vision: State-of-the-Art Review
Authors: Krittanawong Chayakrit , Omar Alaa Mabrouk Salem , Narula Sukrit , Sengupta Partho P. , Glicksberg Benjamin S. , Narula Jagat , Argulian Edgar
Published: April 2023

Exponential growth in data storage and computational power is rapidly narrowing the gap between translating findings from advanced clinical informatics into cardiovascular clinical practice. Specifically, cardiovascular imaging has the distinct advantage in providing a great quantity of data for potentially rich insights, but nuanced interpretation requires a high-level skillset that few individuals possess. A subset of machine learning, deep learning (DL), is a modality that has shown promise, particularly in the areas of image recognition, computer vision, and video classification. Due to a low signal-to-noise ratio, echocardiographic data tend to be challenging to classify; however, utilization of robust DL architectures may help clinicians and researchers automate conventional human tasks and catalyze the extraction of clinically useful data from the petabytes of collected imaging data. The promise is extending far and beyond towards a contactless echocardiographic exam—a dream that is much needed in this time of uncertainty and social distancing brought on by a stunning pandemic culture. In the current review, we discuss state-of-the-art DL techniques and architectures that can be used for image and video classification, and future directions in echocardiographic research in the current era.
Implications of Artificial Intelligence Algorithms in the Diagnosis and Treatment of Motor Neuron Diseases—A Review
Authors: Lopez-Bernal Diego , Balderas David , Ponce Pedro , Rojas Mario , Molina Arturo
Published: April 2023

Motor neuron diseases (MNDs) are a group of chronic neurological disorders characterized by the progressive failure of the motor system. Currently, these disorders do not have a definitive treatment; therefore, it is of huge importance to propose new and more advanced diagnoses and treatment options for MNDs. Nowadays, artificial intelligence is being applied to solve several real-life problems in different areas, including healthcare. It has shown great potential to accelerate the understanding and management of many health disorders, including neurological ones. Therefore, the main objective of this work is to offer a review of the most important research that has been done on the application of artificial intelligence models for analyzing motor disorders. This review includes a general description of the most commonly used AI algorithms and their usage in MND diagnosis, prognosis, and treatment. Finally, we highlight the main issues that must be overcome to take full advantage of what AI can offer us when dealing with MNDs.
Access to Hepatitis C Treatment during and after Incarceration in New Jersey, United States: A Qualitative Study
Authors: Kamat Samir , Kondapalli Sankeerth , Syed Shumayl , Price Gabrielle , Danias George , Gorbenko Ksenia , Cantor Joel , Valera Pamela , Shah Aakash K. , Akiyama Matthew J.
Published: April 2023

Despite effective antiviral therapy for hepatitis C virus (HCV), people who are incarcerated and those returning to the community face challenges in obtaining HCV treatment. We aimed to explore facilitators and barriers to HCV treatment during and after incarceration. From July–November 2020 and June–July 2021, we conducted 27 semi-structured interviews with residents who were formerly incarcerated in jail or prison. The interviews were audio-recorded and professionally transcribed. We used descriptive statistics to characterize the study sample and analyzed qualitative data thematically using an iterative process. Participants included five women and 22 men who self-identified as White (n = 14), Latinx (n = 8), and Black (n = 5). During incarceration, a key facilitator was having sufficient time to complete HCV treatment, and the corresponding barrier was delaying treatment initiation. After incarceration, a key facilitator was connecting with reentry programs (e.g., halfway house or rehabilitation program) that coordinated the treatment logistics and provided support with culturally sensitive staff. Barriers included a lack of insurance coverage and higher-ranking priorities (e.g., managing more immediate reentry challenges such as other comorbidities, employment, housing, and legal issues), low perceived risk of harm related to HCV, and active substance use. Incarceration and reentry pose distinct facilitators and challenges to accessing HCV treatment. These findings signal the need for interventions to improve engagement in HCV care both during and after incarceration to assist in closing the gap of untreated people living with HCV.
Stiffness Modeling and Dynamics Co-Modeling for Space Cable-Driven Linkage Continuous Manipulators
Authors: Xu Hejie , Li Xinliang , Li Yanan , Meng Deshan , Wang Xueqian
Published: April 2023

The space cable-driven continuous manipulator (SCCM) has a slender structure, ultra-high degrees of freedom, and a low mass, which make it suitable for equipment inspection and maintenance operations in an unstructured and limited space environment. In this paper, the SCCM including the cable network and plenty of joint links was deeply modeled. Firstly, the mapping relationship between the cable-driving space, joint space, and task space of the SCCM was studied, and the complete kinematic relationship of the SCCM was established. Secondly, the stiffness components of the SCCM are discussed, and the stiffness modeling method of each part is given. Finally, the Cartesian space equivalent stiffness model of the end was established. Then, a dynamic co-modeling method of Matlab + Adams is proposed, which greatly improved the modeling efficiency while ensuring the modeling accuracy. Finally, based on the stiffness model, the end stiffness characteristics of a specific configuration were analyzed, and the influence of the cable tension on the stiffness and frequency of the manipulator was analyzed. Based on the dynamic co-modeling, the task trajectory dynamics’ simulation analysis and space slit crossing experiment were carried out, which verified that the designed SCCM can meet the needs of slit crossing.
Asymptotic Relations in Applied Models of Inhomogeneous Poisson Point Flows
Authors: Tsitsiashvili Gurami , Osipova Marina
Published: April 2023

A model of a particle flow forming a copy of some image and the distance between the copy and the image are estimated using a special probability metric. The ability of the flow of balls to cover the surface, when grinding the balls, was investigated using formulas of stochastic geometry. Reconstruction of characteristics of an inhomogeneous Poisson flow by inaccurate observations is analysed using the Poisson flow point colouring theorem. The dependence of the Poisson parameter of the distribution of the number of customers in a queuing system with an infinite number of servers and a deterministic service time on the peak load created by an inhomogeneous input Poisson flow is estimated. All these models consist of an inhomogeneous Poisson flow of points and marks glued to each point of the flow and are characterised by their mass, area, volume, observability (or non-observability), and service time. The presence of an asymptotic power–law relationship between model objective functions and parameters of mark crushing is established. These results may be applied in nanotechnology, powder metallurgy, ecology, and consumer services in the implementation of the “Smart City” program. The proposed approach is phenomenological in nature and is justified by the results of real observations and experiments.
Exergy Analysis for Combustible Third-Grade Fluid Flow through a Medium with Variable Electrical Conductivity and Porous Permeability
Authors: Adesanya Samuel O. , Banjo Peace O. , Lebelo Ramoshweu S.
Published: April 2023

A mathematical investigation of a thermodynamical system linked with energy management and its impact on the environment, especially climate change, is presented in this study. In this regard, a numerical investigation of the flow and heat transfer of hydromagnetic third-grade liquid through a porous medium. The permeability of the medium and electrical conductivity of the fluid are assumed to be temperature functions. The appropriate mathematical formulations for momentum, energy, and entropy equations are presented in both dimensional and dimensionless forms. We obtained the numerical solutions using the spectral version of the Chebyshev collocation method and compared the result with the shooting Runge–Kutta method. Numerical results for velocity, temperature, entropy, and Bejan profiles are communicated through tables and graphs with adequate physical interpretation. The thermal stability of the thermo-fluid system that guarantees the prevention of spontaneous fluid heating that fuels climate change is also included in the analysis.
Bayesian Spatial Split-Population Survival Model with Applications to Democratic Regime Failure and Civil War Recurrence
Authors: Joo Minnie M. , Bolte Brandon , Huynh Nguyen , Mukherjee Bumba
Published: April 2023

The underlying risk factors associated with the duration and termination of biological, sociological, economic, or political processes often exhibit spatial clustering. However, existing nonspatial survival models, including those that account for “immune” and “at-risk” subpopulations, assume that these baseline risks are spatially independent, leading to inaccurate inferences in split-population survival settings. In this paper, we develop a Bayesian spatial split-population survival model that addresses these methodological challenges by accounting for spatial autocorrelation among units in terms of their probability of becoming immune and their survival rates. Monte Carlo experiments demonstrate that, unlike nonspatial models, this spatial model provides accurate parameter estimates in the presence of spatial autocorrelation. Applying our spatial model to data from published studies on authoritarian reversals and civil war recurrence reveals that accounting for spatial autocorrelation in split-population models leads to new empirical insights, reflecting the need to theoretically and statistically account for space and non-failure inflation in applied research.
Influence of Pomelo (Citrus maxima) Pericarp Essential Oil on the Physicochemical Properties of HomChaiya Rice (Oryza sativa L. cv. HomChaiya) Flour-Derived Edible Films
Authors: Venkatachalam Karthikeyan , Charoenphun Narin
Published: April 2023

The food industry is increasingly interested in using active edible packaging to address environmental problems caused by conventional synthetic polymers, such as pollution and degradation. The present study took advantage of this opportunity to develop active edible packaging using Hom-Chaiya rice flour (RF), incorporating pomelo pericarp essential oil (PEO) at varying concentrations (1–3%). Films without PEO were used as controls. Various physicochemical parameters, structural and morphological observations were examined in the tested films. Overall, the results showed that the addition of PEO at varying concentrations significantly improved the qualities of the RF edible films, particularly the film’s yellowness (b*) and total color. Furthermore, RF-PEO films with increased concentrations significantly reduced the film’s roughness and relative crystallinity, while increasing opacity. The total moisture content in the films did not differ, but water activity was significantly reduced in the RF-PEO films. Water vapor barrier properties also improved in the RF-PEO films. In addition, textural properties, including tensile strength and elongation at break, were better in the RF-PEO films compared with the control. Fourier-transform infrared spectroscopy (FTIR) revealed strong bonding between the PEO and RF in the film. Morphological studies showed that the addition of PEO smoothed the film’s surface, and this effect increased with concentration. Overall, the biodegradability of the tested films was effective, despite variations; however, a slight advancement in degradation was found in the control film. Lastly, the antimicrobial properties of the RF-PEO films exhibited excellent inhibitory effects against various pathogens, including Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes), Escherichia coli (E. coli), and Salmonella typhimurium (S. typhimurium). This study demonstrated that RF and PEO could be an effective combination for developing active edible packaging that delivers desirable functional properties and excellent biodegradability.
Effect of Temperature on the Kinetics of Localized Plasticity Autowaves in Lüders Deformation
Authors: Danilov Vladimir I. , Orlova Dina V. , Gorbatenko Vadim V. , Danilova Lidiya V.
Published: April 2023

The paper analyzes the elastoplastic transition in Fe–0.025 wt. % C at a temperature of 296–503 K and strain rate of 6.67·10−6–3.33·10−3 s−1. The analysis shows that the lower yield stress increases by a power law with increasing the strain rate, and that its rate sensitivity decreases linearly with increasing the test temperature. At temperatures lower than 393 K, the rate sensitivity of the lower yield stress is normal, and at 393–503 K, it is zero. In the range 393–503 K, the kinetics of the Lüders bands is changed from steady to discrete, and the higher the strain rate, the higher the temperature of this transition. Using the available data on the dynamics of dislocations and diffusion of interstitial impurities in the test alloy, it is demonstrated that the kinetics of Lüders bands are controlled by the effect of dynamic strain aging. If the arrest time of mobile dislocations tw at barriers which are overcome via thermal activation is comparable with the precipitation time of interstitial atoms ta at these dislocations, the motion of a Lüders band is discrete, and the band represents an excitation wave of localized plasticity; its refractory period is determined by the time of dynamic strain aging. If ta >> tw, the band moves monotonically and represents a switching autowave. The results of the analysis suggest that the effect of serrated yielding at the lower temperature boundary of blue brittleness can be suppressed by increasing the strain rate. When the arrest time of dislocations tw decreases, the comparability of tw and ta is broken, and no excitation autowave is formed. The data reported in the paper can be used to develop warm rolling technologies for materials with a sharp elastoplastic transition.
Technologies of Recycling REEs and Iron from NdFeB Scrap
Authors: Xiao Fusheng , Hu Wentao , Zhao Jianqi , Zhu Hongmin
Published: April 2023

In recent years, under the background of global low-carbon development, the production of NdFeB magnets has increased dramatically. With the end of magnet life, a large number of discarded products will be produced in the future. At the same time, 6–73% of industrial waste will be produced in the manufacturing process of magnets. The rare earth content (about 30 wt.%) of these magnet scraps is generally higher than that of raw ore, and the recovery of rare earth elements from them helps to stabilize the global rare earth supply chain. In addition, NdFeB scrap contains about 70 wt.% of iron, which is currently unable to be utilized with high added value. If iron can be recycled based on recycling rare earth elements, it is expected to realize the full component recycling of NdFeB waste and reduce the full life cycle environmental load of NdFeB products. This paper summarizes the properties, recycling potential, and existing recycling technologies of NdFeB waste, and it summarizes the principles, advantages, and disadvantages of various recycling methods, such as direct reuse, pyrometallurgy, hydrometallurgy, and electrochemistry. Among them, the electrochemical recovery method was emphatically reviewed as a newly proposed method. On this basis, the future development direction of NdFeB waste recycling has been prospected, and the research idea of avoiding the shortcomings of various recycling methods through the combined process is proposed. It is proposed that low environmental hazards, low energy consumption, and a closed-loop process are the main goals to be achieved in the recycling process.
Microstructure Evolution and Effect on Deuterium Retention in TiC- and ZrC-Doped Tungsten under He+ Ion Irradiation
Authors: Ding Xiaoyu , Fang Jiahui , Xu Qiu , Zhang Panpan , Zhang Haojie , Luo Laima , Wu Yucheng , Yao Jianhua
Published: April 2023

Combining the advantages of a wet chemical method and spark plasma sintering, carbide-doped materials W-1wt%TiC and W-1wt%ZrC were prepared. Microstructural evolution in W-1wt%TiC and W-1wt%ZrC under irradiation of 5 keV He+ at 600 °C to fluences up to 5.0 × 1021 ions/m2 with ion flux of about 8.8 × 1017 ions/m2s was investigated by transmission electron microscopy (TEM). The dislocation loop number density of W-1wt%TiC was higher than that of W-1wt%ZrC, but the average loop size of the W-1wt%TiC was in average smaller. There were no observable helium bubbles in W-1wt%TiC and W-1wt%ZrC, exhibiting higher radiation resistance to He+ compared to pure W. He+ pre-damaged and undamaged W-1wt%TiC and W-1wt%ZrC samples were irradiated by 5 keV D2+ to estimate the D retention in doped W materials. The irradiation damage impact of He+ on deuterium retention was examined by a method of thermal desorption spectroscopy (TDS). Compared with the undamaged samples, it was illustrated that D2 retention of W-1wt%TiC and W-1wt%ZrC increased after He+ pre-irradiation.
Deregulated Metabolic Pathways in Ovarian Cancer: Cause and Consequence
Authors: Murali Roopak , Balasubramaniam Vaishnavi , Srinivas Satish , Sundaram Sandhya , Venkatraman Ganesh , Warrier Sudha , Dharmarajan Arun , Gandhirajan Rajesh Kumar
Published: April 2023

Ovarian cancers are tumors that originate from the different cells of the ovary and account for almost 4% of all the cancers in women globally. More than 30 types of tumors have been identified based on the cellular origins. Epithelial ovarian cancer (EOC) is the most common and lethal type of ovarian cancer which can be further divided into high-grade serous, low-grade serous, endometrioid, clear cell, and mucinous carcinoma. Ovarian carcinogenesis has been long attributed to endometriosis which is a chronic inflammation of the reproductive tract leading to progressive accumulation of mutations. Due to the advent of multi-omics datasets, the consequences of somatic mutations and their role in altered tumor metabolism has been well elucidated. Several oncogenes and tumor suppressor genes have been implicated in the progression of ovarian cancer. In this review, we highlight the genetic alterations undergone by the key oncogenes and tumor suppressor genes responsible for the development of ovarian cancer. We also summarize the role of these oncogenes and tumor suppressor genes and their association with a deregulated network of fatty acid, glycolysis, tricarboxylic acid and amino acid metabolism in ovarian cancers. Identification of genomic and metabolic circuits will be useful in clinical stratification of patients with complex etiologies and in identifying drug targets for personalized therapies against cancer.
Comparison of the Performance of Density Functional Methods for the Description of Spin States and Binding Energies of Porphyrins
Authors: Morgante Pierpaolo , Peverati Roberto
Published: April 2023

This work analyzes the performance of 250 electronic structure theory methods (including 240 density functional approximations) for the description of spin states and the binding properties of iron, manganese, and cobalt porphyrins. The assessment employs the Por21 database of high-level computational data (CASPT2 reference energies taken from the literature). Results show that current approximations fail to achieve the “chemical accuracy” target of 1.0 kcal/mol by a long margin. The best-performing methods achieve a mean unsigned error (MUE) <15.0 kcal/mol, but the errors are at least twice as large for most methods. Semilocal functionals and global hybrid functionals with a low percentage of exact exchange are found to be the least problematic for spin states and binding energies, in agreement with the general knowledge in transition metal computational chemistry. Approximations with high percentages of exact exchange (including range-separated and double-hybrid functionals) can lead to catastrophic failures. More modern approximations usually perform better than older functionals. An accurate statistical analysis of the results also casts doubts on some of the reference energies calculated using multireference methods. Suggestions and general guidelines for users are provided in the conclusions. These results hopefully stimulate advances for both the wave function and the density functional side of electronic structure calculations.
Occupational Exposure during the Production and the Spray Deposition of Graphene Nanoplatelets-Based Polymeric Coatings
Authors: Bellagamba Irene , Boccuni Fabio , Ferrante Riccardo , Tombolini Francesca , Natale Claudio , Marra Fabrizio , Sarto Maria Sabrina , Iavicoli Sergio
Published: April 2023

Graphene-based polymer composites are innovative materials which have recently found wide application in many industrial sectors thanks to the combination of their enhanced properties. The production of such materials at the nanoscale and their handling in combination with other materials introduce growing concerns regarding workers’ exposure to nano-sized materials. The present study aims to evaluate the nanomaterials emissions during the work phases required to produce an innovative graphene-based polymer coating made of a water-based polyurethane paint filled with graphene nanoplatelets (GNPs) and deposited via the spray casting technique. For this purpose, a multi-metric exposure measurement strategy was adopted in accordance with the harmonized tiered approach published by the Organization for Economic Co-operation and Development (OECD). As a result, potential GNPs release has been indicated near the operator in a restricted area not involving other workers. The ventilated hood inside the production laboratory guarantees a rapid reduction of particle number concentration levels, limiting the exposure time. Such findings allowed us to identify the work phases of the production process with a high risk of exposure by inhalation to GNPs and to define proper risk mitigation strategies.
Utilizing Imine Bonds to Create a Self-Gated Mesoporous Silica Material with Controlled Release and Antimicrobial Properties
Authors: Lu Yuyang , Li Xutao , Xu Jiaqi , Sun Huimin , Sheng Jie , Song Yishan , Chen Yang
Published: April 2023

In recent years, silica nanomaterials have been widely studied as carriers in the field of antibacterial activity in food. Therefore, it is a promising but challenging proposition to construct responsive antibacterial materials with food safety and controllable release capabilities using silica nanomaterials. In this paper, a pH-responsive self-gated antibacterial material is reported, which uses mesoporous silica nanomaterials as a carrier and achieves self-gating of the antibacterial agent through pH-sensitive imine bonds. This is the first study in the field of food antibacterial materials to achieve self-gating through the chemical bond of the antibacterial material itself. The prepared antibacterial material can effectively sense changes in pH values caused by the growth of foodborne pathogens and choose whether to release antibacterial substances and at what rate. The development of this antibacterial material does not introduce other components, ensuring food safety. In addition, carrying mesoporous silica nanomaterials can also effectively enhance the inhibitory ability of the active substance.
Editorial for the Special Issue “Applications of Optical Thin Films”
Authors: Hsu Jin-Cherng
Published: April 2023

Optical thin films have been vital to enhancing optical performance for many years [...]
Debridement, Antibiotic Pearls, and Retention of the Implant (DAPRI) in the Treatment of Early Periprosthetic Joint Infections: A Consecutive Series
Authors: Indelli Pier , Ghirardelli Stefano , Valpiana Pieralberto , Bini Lorenzo , Festini Michele , Iannotti Ferdinando
Published: April 2023

Introduction: Periprosthetic joint infections (PJI) represent a devastating consequence following total joint arthroplasty (TJA). In this study, the authors describe a modified surgical technique developed to enhance the classical irrigation and debridement procedure (DAIR) to improve the possibilities of retaining an acutely infected TJA. Materials and Methods: This technique, debridement antibiotic pearls and retention of the implant (DAPRI), aims to remove the intra-articular biofilm allowing a higher and prolonged local antibiotic concentration by using calcium sulphate antibiotic-added beads in a setting of acute (<4 weeks from symptoms onset) PJI with pathogen identification. The combination of three different surgical techniques (tumor-like synovectomy, argon beam/acetic acid application and chlorhexidine gluconate brushing) aims to remove the bacterial biofilm from the implant without explanting the original hardware. Results: In total, 62 patients met the acute infection criteria (<4 weeks of symptoms); there were 57 males and five females. The patients’ average age at the time of treatment was 71 years (62–77) and the average BMI was 37 kg/m2. The micro-organism, always identified through synovial fluid analysis (culture, multiplex PCR or Next Generation Sequencing), was an aerobic Gram + in 76% (S. Coag-Neg 41%; S. aureus 16%), Gram—in 10% (E. coli 4%) and anaerobic Gram + in 4%. The DAPRI treatment was performed at an average of 3 days from symptoms onset (1–7 days). All patients underwent a 12-week course of post-operative antibiotic therapy (6 weeks I.V. and 6 weeks oral). All patients were available at the 2-year minimum FU (24–84 months). A total of 48 (77.5%) patients were infection-free at the final FU, while 14 patients underwent 2-stage revision for PJI recurrence. In total, four patients (6.4%) had a prolonged drainage from the wound after placement of the calcium sulphate beads. Conclusions: This study suggests that the DAPRI technique could represent a valid alternative to the classic DAIR procedure. The current authors do not recommend this procedure outside of the main inclusive criteria (acute scenario micro-organism identification).
Potential Inhibitory Effect of Miltefosine against Terbinafine-Resistant Trichophyton indotineae
Authors: Haghani Iman , Akhtari Javad , Yahyazadeh Zahra , Espahbodi Amirreza , Kermani Firoozeh , Javidnia Javad , Hedayati Mohammad Taghi , Shokohi Tahereh , Badali Hamid , Rezaei-Matehkolaei Ali , Aghili Seyed Reza , Al-Rawahi Ahmed , Al-Harrasi Ahmed , Abastabar Mahdi , Al-Hatmi Abdullah M. S.
Published: April 2023

Several prolonged and significant outbreaks of dermatophytosis caused by Trichophyton indotineae, a new emerging terbinafine-resistant species, have been ongoing in India in recent years, and have since spread to various countries outside Asia. Miltefosine, an alkylphosphocholine, is the most recently approved drug for the treatment of both visceral and cutaneous leishmaniasis. Miltefosine in vitro activity against terbinafine-resistant and susceptible T. mentagrophytes/T. interdigitale species complex, including T. indotineae, is limited. The current study aimed to assess miltefosine’s in vitro activity against dermatophyte isolates, which are the most common causes of dermatophytosis. Miltefosine, terbinafine, butenafine, tolnaftate, and itraconazole susceptibility testing was performed using Clinical and Laboratory Standards Institute broth microdilution methods (CLSI M38-A3) against 40 terbinafine-resistant T. indotineae isolates and 40 terbinafine-susceptible T. mentagrophytes/T. interdigitale species complex isolates. Miltefosine had MIC ranges of 0.063–0.5 µg/mL and 0.125–0.25 µg/mL against both terbinafine-resistant and susceptible isolates. In terbinafine-resistant isolates, the MIC50 and MIC90 were 0.125 µg/mL and 0.25 µg/mL, respectively, and 0.25 µg/mL in susceptible isolates. Miltefosine had statistically significant differences in MIC results when compared to other antifungal agents (p-value 0.05) in terbinafine-resistant strains. Accordingly, the findings suggest that miltefosine has a potential activity for treating infections caused by terbinafine-resistant T. indotineae. However, further studies are needed to determine how well this in vitro activity translates into in vivo efficacy.
Statistical Properties of Partially Coherent Higher-Order Laguerre-Gaussian Power-Exponent Phase Vortex Beams
Authors: Ma Zhiyuan , Pan Yuqi , Dou Jiantai , Zhao Jiang , Li Bo , Hu Youyou
Published: April 2023

In this paper, partially coherent radially polarized (RP) Laguerre-Gaussian (LG) rotationally symmetrical power-exponent phase vortex (RSPEPV) beams with the LG-correlated Schell-model (LGSM) were introduced. The statistical properties of the tightly focused beams, including intensity distribution, degrees of polarization and coherence, and Stokes vector, were studied based on vectorial Richards-Wolf diffraction integral theory. Moreover, when the distance between focal plane and the observation plane z = 0, the relationships between the tight-focusing properties of RP-LG-RSPEPV beams with LGSM and the order of LGSM p’, topological charges l, power exponent n, spatial correlation δ, and radial index p were investigated. The results show that by changing the order of LGSM, topological charge, power exponent, spatial correlation length, and radial index, the focal spot distribution of various shapes can be obtained. This work provides ideas for the application of partially coherent beams in particle capture and optical tweezers.
Buckwheat Flower Volatiles Attract Peristenus spretus and Enhance Its Field-Level Parasitism of Apolygus lucorum
Authors: Xia Shike , Zhang Tao , Williams Livy , Yang Yizhong , Lu Yanhui
Published: April 2023

Volatile compounds play indispensable roles in the interactions among host plants, herbivores and natural enemies. Previous studies showed that the addition of buckwheat strips in cotton fields could attract Peristenus spretus, the dominant parasitoid of Apolygus lucorum, and enhance its parasitic activity. Through the combined analysis of Y-tube olfactometer, solid-phase microextraction (SPME), gas chromatography-mass spectrometer (GC-MS) and electroantennography (EAG), we found that male and female P. spretus responded to compounds present in buckwheat flowers. The five major components of buckwheat flowers, cis-3-hexenyl acetate (Z3HA), 4-methylanisole, 4-oxoisophorone, p-methylphenol and 2-ethylhexyl salicylate, all had a significant attraction to P. spretus adults and led to positive electroantennogram responses, especially for 10 mg/mL 4-oxoisophorone, indicating the components played a key role in the selection behavior of P. spretus to buckwheat flowers. Additionally, field trials showed that the five volatiles could significantly increase the parasitism by P. spretus. Our study screened the key active components of buckwheat flower volatiles that have an attractive effect on P. spretus, revealing its behavioral selection mechanism and emphasizing the important role of plant volatiles on host selection and parasitism of parasitic wasps, providing a theoretical basis for the development of attractants for P. spretus and the reduction of pesticides in the field to promote conservation biological control (CBC) of A. lucorum.
Grapefruit Root and Rhizosphere Responses to Varying Planting Densities, Fertilizer Concentrations and Application Methods
Authors: Santiago John M. , Kadyampakeni Davie M. , Fox John-Paul , Wright Alan L. , Guzmán Sandra M. , Ferrarezi Rhuanito Soranz , Rossi Lorenzo
Published: April 2023

Huanglongbing (HLB) disease has caused a severe decline in citrus production globally over the past decade. There is a need for improved nutrient regimens to better manage the productivity of HLB-affected trees, as current guidelines are based on healthy trees. The aim of this study was to evaluate the effects of different fertilizer application methods and rates with different planting densities on HLB-affected citrus root and soil health. Plant material consisted of ‘Ray Ruby’ (Citrus × paradisi) grapefruit trees grafted on ‘Kuharske’ citrange (Citrus × sinensis × Citrus trifoliata). The study consisted of 4 foliar fertilizer treatments, which included 0×, 1.5×, 3× and 6× the University of Florida Institute of Food and Agriculture (UF/IFAS) recommended guidelines for B, Mn and Zn. Additionally, 2 ground-applied fertilizer treatments were used, specifically controlled-release fertilizer (CRF1): 12−3−14 + B, Fe, Mn and Zn micronutrients at 1× UF/IFAS recommendation, and (CRF2): 12−3−14 + 2× Mg + 3× B, Fe, Mn and Zn micronutrients, with micronutrients applied as sulfur-coated products. The planting densities implemented were low (300 trees ha−1), medium (440 trees ha−1) and high (975 trees ha−1). The CRF fertilizer resulted in greater soil nutrient concentrations through all of the time sampling points, with significant differences in soil Zn and Mn. Grapefruit treated with ground-applied CRF2 and 3× foliar fertilizers resulted in the greatest bacterial alpha and beta diversity in the rhizosphere. Significantly greater abundances of Rhizobiales and Vicinamibacterales were found in the grapefruit rhizosphere of trees treated with 0× UF/IFAS foliar fertilizer compared to higher doses of foliar fertilizers.
A Genome-Wide Association Study of Protein, Oil, and Amino Acid Content in Wild Soybean (Glycine soja)
Authors: Kim Woon Ji , Kang Byeong Hee , Kang Sehee , Shin Seoyoung , Chowdhury Sreeparna , Jeong Soon-Chun , Choi Man-Soo , Park Soo-Kwon , Moon Jung-Kyung , Ryu Jaihyunk , Ha Bo-Keun
Published: April 2023

Soybean (Glycine max L.) is a globally important source of plant proteins, oils, and amino acids for both humans and livestock. Wild soybean (Glycine soja Sieb. and Zucc.), the ancestor of cultivated soybean, could be a useful genetic source for increasing these components in soybean crops. In this study, 96,432 single-nucleotide polymorphisms (SNPs) across 203 wild soybean accessions from the 180K Axiom® Soya SNP array were investigated using an association analysis. Protein and oil content exhibited a highly significant negative correlation, while the 17 amino acids exhibited a highly significant positive correlation with each other. A genome-wide association study (GWAS) was conducted on the protein, oil, and amino acid content using the 203 wild soybean accessions. A total of 44 significant SNPs were associated with protein, oil, and amino acid content. Glyma.11g015500 and Glyma.20g050300, which contained SNPs detected from the GWAS, were selected as novel candidate genes for the protein and oil content, respectively. In addition, Glyma.01g053200 and Glyma.03g239700 were selected as novel candidate genes for nine of the amino acids (Ala, Asp, Glu, Gly, Leu, Lys, Pro, Ser, and Thr). The identification of the SNP markers related to protein, oil, and amino acid content reported in the present study is expected to help improve the quality of selective breeding programs for soybeans.
Effect of Green Synthesized ZnO-NPs on Growth, Antioxidant System Response and Bioactive Compound Accumulation in Echinops macrochaetus, a Potential Medicinal Plant, and Assessment of Genome Size (2C DNA Content)
Authors: Khan Salim , Al-Qurainy Fahad , Al-hashimi Abdulrahman , Nadeem Mohammad , Tarroum Mohamed , Shaikhaldein Hassan O. , Salih Abdalrhaman M.
Published: April 2023

Echinops macrochaetus is a medicinal plant that can be used to cure various diseases. In the present study, plant-mediated zinc oxide nanoparticles (ZnO-NPs) were synthesized using an aqueous leaf extract of the medicinal plant Heliotropium bacciferum and characterized using various techniques. E. macrochaetus was collected from the wild and identified using the internal transcribed spacer sequence of nrDNA (ITS-nrDNA), which showed the closeness to its related genus in a phylogenetic tree. The effect of synthesized biogenic ZnO-NPs was studied on E. macrochaetus in a growth chamber for growth, bioactive compound enhancement and antioxidant system response. The irrigation of plants at a low concentration of ZnO-NPs (T1 = 10 mg/L) induced more growth in terms of biomass, chlorophyll content (273.11 µg/g FW) and carotenoid content (135.61 µg/g FW) than the control and other treatments (T2-20 mg/L and T3-40 mg/L). However, the application of a high concentration of ZnO-NPs (20 and 40 mg/L) increased the level of antioxidant enzymes (SOD, APX and GR), total crude and soluble protein, proline and TBARS contents. The accumulations of the compounds quercetin-3-β-D-glucoside, luteolin 7-rutinoside and p-coumaric acid were greater in the leaf compared to the shoot and root. A minor variation was observed in genome size in treated plants as compared to the control group. Overall, this study revealed the stimulatory effect of phytomediated ZnO-NPs, which act as bio-stimulants/nano-fertilizers as revealed by more biomass and the higher production of phytochemical compounds in different parts of the E. macrochaetus.
Modeling and Multi-Stage Planning of Cement-IIES Considering Carbon-Green Certificate Trading
Authors: Guo Zhaochu , Zhou Suyang
Published: April 2023

The cement industry is an important industrial entity responsible for implementing carbon emission reduction targets. Considering the carbon trading and green certificate trading mechanisms, this paper presents a multi-stage planning approach for the constructed Cement-Industrial Integrated Energy System (Cement-IIES). Carbon reduction technologies represented by low-temperature waste heat recovery, as well as phased changes in economic and technical parameters, are considered in the model. The case study shows that the proposed method not only optimizes the design economy of the Cement-IIES but also achieves a substantial carbon emission reduction in the cement production process and energy supply system. Compared with the traditional single-stage planning, the proposed method improves the system’s economic efficiency by 13.88% and flexibly adapts to changes in policies such as “coal reform”, green certificate trading and carbon quotas. The low-temperature waste heat recovery technology helps the system energy utilization efficiency in the two stages increase by 0.45% and 0.86%, respectively, whilst oxygen-enriched combustion and carbon capture technologies can reduce the total carbon emissions by about 83%. In addition, the negative carbon emission effect of biomass gives the system access to annual benefits of CNY 3.10 × 107 and CNY 7.89 × 107 in the two stages, respectively.
SAR-AD Method to Characterize Eight SARA Fractions in Various Vacuum Residues and Follow Their Transformations Occurring during Hydrocracking and Pyrolysis
Authors: Adams Jeramie J. , Rovani Joseph F. , Planche Jean-Pascal , Loveridge Jenny , Literati Alex , Shishkova Ivelina , Palichev Georgi , Kolev Iliyan , Atanassov Krassimir , Nenov Svetoslav , Ribagin Simeon , Stratiev Danail , Yordanov Dobromir , Huo Jianqiang
Published: April 2023

Model compounds were used to provide some chemical boundaries for the eight-fraction SAR-ADTM characterization method for heavy oils. It was found that the Saturates fraction consists of linear and highly cyclic alkanes; the Aro-1 fraction consists of molecules with a single aromatic ring; the Aro-2 fraction consists of mostly 2 and 3-ring fused aromatic molecules, the pericondensed 4-ring molecule pyrene, and molecules with 3–5 rings that are not fused; and the Aro-3 fraction consists of 4-membered linear and catacondensed aromatics, larger pericondensed aromatics, and large polycyclic aromatic hydrocarbons. The Resins fraction consists of mostly fused aromatic ring systems containing polar functional groups and metallated polar vanadium oxide porphyrin compounds, and the Asphaltene fraction consists of both island- and archipelago-type structures with a broad range of molecular weight variation, aromaticity, and heteroatom contents. The behavior of the eight SAR-ADTM fractions during hydrocracking and pyrolysis was investigated, and quantitative relations were established. Intercriteria analysis and evaluation of SAR-ADTM data of hydrocracked vacuum residue and sediment formation rate in commercial ebullated bed vacuum residue hydrocracking were performed. It showed that total asphaltene content, toluene-soluble asphaltenes, and colloidal instability index contribute to sediment formation, while Resins and Cyclohexane-soluble asphaltenes had no statistically meaningful relation to sediment formation for the studied range of operation conditions.
Effect of Processing Technology on Mechanical Properties of HRB500 High-Strength Steel Bar Threading
Authors: Niu Huli , Yang Jiajun , Yang Shuo , Zhang Jiayu , Yan Haipeng , Zhao Wanqing
Published: April 2023

The processing quality of steel bar thread has a large influence on its performance. Using the traditional thread processing technology, it is difficult to meet the requirements of steel bar thread processing with large diameter and high strength. A technical process for HRB500 high-strength steel bar thread processing, including face milling, rib stripping, chamfering, necking formation, and thread rolling, was proposed. The influences of cutting parameters on the cutting force of steel bar surface in face milling were analyzed by the finite element method. For the necking formation process, the effect of springback amount on necking formation was studied. The main parameters in rolling formation were analyzed and calculated, including extrusion pressure, rolling speed, and rolling feed. Experiments for uniaxial tensile of the processed high-strength steel bar threads were carried out. The results showed that cutting depth has the largest influence on the cutting force; the second is feed rate. The effect of the spindle speed was lowest during the face milling. After the necking formation process, the values of the maximum springback amount along the X, Y, and Z directions were 0.05 mm, 0.06 mm, and 0.98 mm, respectively. The finished thread met the precision and quality requirements of a grade I joint. This study provides a high-quality processing technology for large-diameter and high-strength steel bar threads.
Advancing Process Intensification with High-Frequency Ultrasound: A Mini-Review of Applications in Biofuel Production and Beyond
Authors: Chit Viesuieda , Tan Lian See , Kiew Peck Loo , Tsuji Tomoya , Funazukuri Toshitaka , Lock Serene Sow Mun
Published: April 2023

High-frequency ultrasound (HFU) is an ultrasound technology with a frequency higher than 1000 kHz. It has become increasingly recognized as an emerging process intensification technology in various fields, such as biofuel production, carbon dioxide absorption, and wastewater treatment. HFU is seen as a potential intensifier technology for biofuel production, as its mechanisms, such as cavitational phenomena, microstreaming, and fountain formation, can benefit biofuel production. Previous research has shown that HFU can decrease the reaction time required for biofuel production, aid in lipid extraction, increase carbon dioxide absorption rates, and be effective in destroying pathogens in wastewater treatment. However, despite the potential benefits, there are limited reports on the use of HFU technology for biofuel production, which has led to uncertainties and constraints in its industrial deployment. These constraints include equipment design, economic analysis, and safety concerns, which require further in-depth analysis. Despite these limitations, previous studies have shown promising results for the incorporation of HFU into various fields due to its unique characteristics and mechanisms. This paper presents a review of the theory and application of HFU for process intensification, with a focus on its potential for biofuel production. It also provides recommendations for the further exploration of the technology to overcome industrial deployment obstacles.
Single Particle Inductively Coupled Plasma Time-of-Flight Mass Spectrometry—A Powerful Tool for the Analysis of Nanoparticles in the Environment
Authors: Meng Ziwei , Zheng Lingna , Fang Hao , Yang Pu , Wang Bing , Li Liang , Wang Meng , Feng Weiyue
Published: April 2023

Single-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) has emerged as an important tool for the characterization of inorganic nanoparticles (NPs) in the environment. Although most SP-ICP-MS applications rely on the quadrupole ICP-MS (ICP-QMS), it is limited by the slow scanning speed of the quadrupole. Recent advancements in instrumentation have led to the development of inductively coupled plasma time-of-flight mass spectrometry (ICP-TOF-MS) which offers a viable solution. In this review, we discuss the recent advances in instrumentation and methodology of ICP-TOF-MS, followed by a detailed discussion of the applications of SP-ICP-TOFMS in analyzing NPs in the environment. SP-ICP-TOFMS has the potential to identify and quantify both anthropogenic and natural NPs in the environment, providing valuable insights into their occurrence, fate, behavior, and potential environmental risks.