bluuebunny/crossref_metadata_2025 huggingface dataset:
Rendering Strategy to Counter Mutual Masking Effect in Multiple Tactile Feedback
Authors: Ryu Semin , Pyo Dongbum , Lim Soo-Chul , Kwon Dong-Soo
Published: July 2020

Recently, methods and devices that simultaneously utilize two or more tactile feedback types have been proposed for more immersive interaction with virtual objects. However, the masking effect, which makes us less sensitive to various stimuli presented at the same time, has scarcely been explored. In this study, we propose a novel tactile rendering algorithm that can eliminate the mutual masking effect at the user’s sensation level, when mechanical vibration and electrovibration are applied simultaneously. First, the masking functions of the two stimuli were investigated for various stimulus combinations. Based on these, a generalized form of the masking function was derived. We then tested and confirmed that the proposed algorithm, which calculates the required stimulus intensity to compensate for the mutual masking effect, could render the arbitrary stimulus intensity desired to be perceived by the users. The results of the user test revealed that the proposed rendering algorithm significantly improved the virtual object recognition rate by approximately 23% when geometry and texture were presented jointly. This finding suggests principal guidelines for the combined use of mechanical vibration and electrovibration, as well as for other combinations of different tactile feedback types.
Investigating the Interannual Variability of the Boreal Summer Water Vapor Source and Sink over the Tropical Eastern Indian Ocean-Western Pacific
Authors: Zou Meng , Qiao Shaobo , Chao Liya , Chen Dong , Hu Chundi , Li Qingxiang , Feng Guolin
Published: July 2020

Using the four-times daily and monthly-mean reanalysis datasets of NCEP/NCAR for the 1958 to 2018 period, we investigate the interannual variability of the June-July-August (JJA)–mean water vapor source and sink over the tropical eastern Indian Ocean-Western Pacific (TEIOWP) and the underlying mechanism. It is found that the two major modes (EOF1 and EOF2) of the water vapor source and sink anomalies over the TEIOWP present a southwest-northeast oriented dipole and a southwest-northeast oriented tripole. Specifically, when the western maritime continent shows an anomalous water vapor source, the northwestern Pacific is characterized by anomalous water vapor sink and source in EOF1 and EOF2 modes, respectively. The EOF1 and EOF2 modes are primarily driven by a single and a double meridional cell anomaly, which corresponds to the in-phase and out-of-phase linkage between evaporation anomalies over the western maritime continent and precipitation anomalies over the northwestern Pacific, respectively. Furthermore, the EOF1 mode is regulated by the quick transition of the El Niño-Southern Oscillation (ENSO) phase, whereas the EOF2 mode probably originates from internal atmospheric variability. Considering that the standard deviation of PC1 is much higher during ENSO years than that during non-ENSO years, it is probable that the water source and sink anomalies over the TEIOWP tend to be dominant by EOF1 mode during ENSO years. In contrast, the EOF2 mode may play an important role in the water source and sink anomalies over the TEIOWP during non-ENSO years. Accordingly, the water vapor source and sink anomalies over the TEIOWP may be well predicted based on the ENSO state in the previous December-January-February. These results are useful for understanding the predictability of water vapor source and sink anomalies over the TEIOWP.
Testosterone Decreases Placental Mitochondrial Content and Cellular Bioenergetics
Authors: Mishra Jay S. , Blesson Chellakkan S. , Kumar Sathish
Published: July 2020

Placental mitochondrial dysfunction plays a central role in the pathogenesis of preeclampsia. Since preeclampsia is a hyperandrogenic state, we hypothesized that elevated maternal testosterone levels induce damage to placental mitochondria and decrease bioenergetic profiles. To test this hypothesis, pregnant Sprague–Dawley rats were injected with vehicle or testosterone propionate (0.5 mg/kg/day) from gestation day (GD) 15 to 19. On GD20, the placentas were isolated to assess mitochondrial structure, copy number, ATP/ADP ratio, and biogenesis (Pgc-1α and Nrf1). In addition, in vitro cultures of human trophoblasts (HTR-8/SVneo) were treated with dihydrotestosterone (0.3, 1.0, and 3.0 nM), and bioenergetic profiles using seahorse analyzer were assessed. Testosterone exposure in pregnant rats led to a 2-fold increase in plasma testosterone levels with an associated decrease in placental and fetal weights compared with controls. Elevated maternal testosterone levels induced structural damage to the placental mitochondria and decreased mitochondrial copy number. The ATP/ADP ratio was reduced with a parallel decrease in the mRNA and protein expression of Pgc-1α and Nrf1 in the placenta of testosterone-treated rats compared with controls. In cultured trophoblasts, dihydrotestosterone decreased the mitochondrial copy number and reduced PGC-1α, NRF1 mRNA, and protein levels without altering the expression of mitochondrial fission/fusion genes. Dihydrotestosterone exposure induced significant mitochondrial energy deficits with a dose-dependent decrease in basal respiration, ATP-linked respiration, maximal respiration, and spare respiratory capacity. In summary, our study suggests that the placental mitochondrial dysfunction induced by elevated maternal testosterone might be a potential mechanism linking preeclampsia to feto-placental growth restriction.
The Gintonin-Enriched Fraction of Ginseng Regulates Lipid Metabolism and Browning via the cAMP-Protein Kinase a Signaling Pathway in Mice White Adipocytes
Authors: Lee Kippeum , Jin Heegu , Chei Sungwoo , Oh Hyun-Ji , Choi Sun-Hye , Nah Seung-Yeol , Lee Boo-Yong
Published: July 2020

Obesity is a major health concern and is becoming an increasingly serious societal problem worldwide. The browning of white adipocytes has received considerable attention because of its potential protective effect against obesity-related metabolic disease. The gintonin-enriched fraction (GEF) is a non-saponin, glycolipoprotein component of ginseng that is known to have neuroprotective and anti-inflammatory effects. However, the anti-obesity and browning effects of GEF have not been explored to date. Therefore, we aimed to determine whether GEF has a preventive effect against obesity. We differentiated 3T3-L1 cells and mouse primary subcutaneous adipocytes for 8 days in the presence or absence of GEF, and then measured the expression of intermediates in signaling pathways that regulate triglyceride (TG) synthesis and browning by Western blotting and immunofluorescence analysis. We found that GEF reduced lipid accumulation by reducing the expression of pro-adipogenic and lipogenic factors, and increased lipolysis and thermogenesis, which may be mediated by an increase in the phosphorylation of protein kinase A. These findings suggest that GEF may induce fat metabolism and energy expenditure in white adipocytes and therefore may represent a potential treatment for obesity.
Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value
Authors: Fahim Sarah Atef , Abdullah Mahmoud Salah , Espinoza-Sánchez Nancy A. , Hassan Hebatallah , Ibrahim Ayman M. , Ahmed Sarah Hamdy , Shakir George , Badawy Mohamed A. , Zakhary Nadia I. , Greve Burkhard , El-Shinawi Mohamed , Götte Martin , Ibrahim Sherif Abdelaziz
Published: July 2020

Inflammatory breast cancer (IBC) is a rare yet aggressive breast cancer variant, associated with a poor prognosis. The major challenge for IBC is misdiagnosis due to the lack of molecular biomarkers. We profiled dysregulated expression of microRNAs (miRNAs) in primary samples of IBC and non-IBC tumors using human breast cancer miRNA PCR array. We discovered that 28 miRNAs were dysregulated (10 were upregulated, while 18 were underexpressed) in IBC vs. non-IBC tumors. We identified 128 hub genes, which are putative targets of the differentially expressed miRNAs and modulate important cancer biological processes. Furthermore, our qPCR analysis independently verified a significantly upregulated expression of miR-181b-5p, whereas a significant downregulation of miR-200b-3p, miR-200c-3p, and miR-203a-3p was detected in IBC tumors. Receiver operating characteristic (ROC) curves implied that the four miRNAs individually had a diagnostic accuracy in discriminating patients with IBC from non-IBC and that miR-203a-3p had the highest diagnostic value with an AUC of 0.821. Interestingly, a combination of miR-181b-5p, miR-200b-3p, and miR-200c-3p robustly improved the diagnostic accuracy, with an area under the curve (AUC) of 0.897. Intriguingly, qPCR revealed that the expression of zinc finger E box-binding homeobox 2 (ZEB2) mRNA, the putative target of miR-200b-3p, miR-200c-3p, and miR-203a-3p, was upregulated in IBC tumors. Overall, this study identified a set of miRNAs serving as potential biomarkers with diagnostic relevance for IBC.
Proteomic and Bioinformatic Investigations of Heat-Treated Anisakis simplex Third-Stage Larvae
Authors: Kochanowski Maciej , Różycki Mirosław , Dąbrowska Joanna , Bełcik Aneta , Karamon Jacek , Sroka Jacek , Cencek Tomasz
Published: July 2020

Anisakis simplex third-stage larvae are the main source of hidden allergens in marine fish products. Some Anisakis allergens are thermostable and, even highly processed, could cause hypersensitivity reactions. However, Anisakis proteome has not been studied under autoclaving conditions of 121 °C for 60 min, which is an important process in the food industry. The aim of the study was the identification and characterization of allergens, potential allergens, and other proteins of heat-treated A. simplex larvae. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify 470 proteins, including allergens—Ani s 1, Ani s 2, Ani s 3, Ani s 4, Ani s 5—and 13 potential allergens that were mainly homologs of Anisakis spp., Ascaris spp., and Acari allergens. Ani s 2, Ani s 3, Ani s 5, and three possible allergens were found among the top 25 most abundant proteins. The computational analysis allowed us to detect allergen epitopes, assign protein families, and domains as well as to annotate the localization of proteins. The predicted 3D models of proteins revealed similarities between potential allergens and homologous allergens. Despite the partial degradation of heated A. simplex antigens, their immunoreactivity with anti-A. simplex IgG antibodies was confirmed using a Western blot. In conclusion, identified epitopes of allergenic peptides highlighted that the occurrence of Anisakis proteins in thermally processed fish products could be a potential allergic hazard. Further studies are necessary to confirm the IgE immunoreactivity and thermostability of identified proteins.
Adipose Tissue-Derived Stem Cells Retain Their Adipocyte Differentiation Potential in Three-Dimensional Hydrogels and Bioreactors
Authors: O'Donnell Benjamen , Al-Ghadban Sara , Ives Clara , L'Ecuyer Michael , Monjure Tia , Romero-Lopez Monica , Li Zhong , Goodman Stuart , Lin Hang , Tuan Rocky , Bunnell Bruce
Published: July 2020

Osteoarthritis (OA) is a common joint disorder with a significant economic and healthcare impact. The knee joint is composed of cartilage and the adjoining bone, a synovial capsule, the infrapatellar fat pad (IPFP), and other connective tissues such as tendons and ligaments. Adipose tissue has recently been highlighted as a major contributor to OA through strong inflammation mediating effects. In this study, methacrylated gelatin (GelMA) constructs seeded with adipose tissue-derived mesenchymal stem cells (ASCs) and cultured in a 3D printed bioreactor were investigated for use in microphysiological systems to model adipose tissue in the knee joint. Four patient-derived ASC populations were seeded at a density of 20 million cells/mL in GelMA. Live/Dead and boron-dipyrromethene/4′,6-diamidino-2-phenylindole (BODIPY/DAPI) staining of cells within the constructs demonstrated robust cell viability after 28 days in a growth (control) medium, and robust cell viability and lipid accumulation in adipogenic differentiation medium. qPCR gene expression analysis and protein analysis demonstrated an upregulated expression of key adipogenesis-associated genes. Overall, these data indicate that ASCs retain their adipogenic potential when seeded within GelMA hydrogels and cultured within perfusion bioreactors, and thus can be used in a 3D organ-on-a-chip system to study the role of the IPFP in the pathobiology of the knee OA.
Regulated Iron Siderophore Production of the Halophilic Archaeon Haloferax volcanii
Authors: Niessen Natalie , Soppa Jörg
Published: July 2020

Iron is part of many redox and other enzymes and, thus, it is essential for all living beings. Many oxic environments have extremely low concentrations of free iron. Therefore, many prokaryotic species evolved siderophores, i.e., small organic molecules that complex Fe3+ with very high affinity. Siderophores of bacteria are intensely studied, in contrast to those of archaea. The haloarchaeon Haloferax volcanii contains a gene cluster that putatively encodes siderophore biosynthesis genes, including four iron uptake chelate (iuc) genes. Underscoring this hypothesis, Northern blot analyses revealed that a hexacistronic transcript is generated that is highly induced under iron starvation. A quadruple iuc deletion mutant was generated, which had a growth defect solely at very low concentrations of Fe3+, not Fe2+. Two experimental approaches showed that the wild type produced and exported an Fe3+-specific siderophore under low iron concentrations, in contrast to the iuc deletion mutant. Bioinformatic analyses revealed that haloarchaea obtained the gene cluster by lateral transfer from bacteria and enabled the prediction of enzymatic functions of all six gene products. Notably, a biosynthetic pathway is proposed that starts with aspartic acid, uses several group donors and citrate, and leads to the hydroxamate siderophore Schizokinen.
Increased IL-2 and Reduced TGF-β Upon T-Cell Stimulation are Associated with GM-CSF Upregulation in Multiple Immune Cell Types in Multiple Sclerosis
Authors: Aram Jehan , Frakich Nanci , Morandi Elena , Alrouji Mohammed , Samaraweera Amal , Onion David , Spendlove Ian , Colombo Sergio L. , Tanasescu Radu , Gran Bruno , Constantinescu Cris S.
Published: July 2020

Granulocyte macrophage colony stimulating factor (GM-CSF) is a pro-inflammatory cytokine produced by immune cells. Recent evidence suggests that GM-CSF plays an important role in multiple sclerosis (MS) pathogenesis. We investigated the expression and regulation of GM-CSF in different immune cells in MS. We also investigated the differentiation and frequency of GM-CSF-producing Th cells that do not co-express interferon (IFN)-γ or interleukin-17 (IL-17) (Th-GM cells) in MS. We found a significant increase in the percentage of GM-CSF-expressing Th cells, Th1 cells, Th-GM cells, cytotoxic T (Tc) cells, monocytes, natural killer (NK) cells, and B cells in PBMC from MS patients stimulated with T cell stimuli. Stimulated PBMC culture supernatants from MS patients contained significantly higher levels of IL-2, IL-12, IL-1β, and GM-CSF and significantly lower levels of transforming growth factor (TGF-)β. Blocking IL-2 reduced the frequency of Th-GM cells in PBMC from MS patients. The frequency of Th-GM cells differentiated in vitro from naïve CD4+ T cells was significantly higher in MS patients and was further increased in MS with IL-2 stimulation. These findings suggest that all main immune cell subsets produce more GM-CSF in MS after in vitro stimulation, which is associated with defective TGF-β and increased IL-2 and IL-12 production. Th-GM cells are increased in MS. GM-CSF may be a potential therapeutic target in MS.
Sphingosine-1-Phosphate: Its Pharmacological Regulation and the Treatment of Multiple Sclerosis: A Review Article
Authors: Cohan Stanley , Lucassen Elisabeth , Smoot Kyle , Brink Justine , Chen Chiayi
Published: July 2020

Sphingosine-1-phosphate (S1P), via its G-protein-coupled receptors, is a signaling molecule with important regulatory properties on numerous, widely varied cell types. Five S1P receptors (S1PR1-5) have been identified, each with effects determined by their unique G-protein-driven downstream pathways. The discovery that lymphocyte egress from peripheral lymphoid organs is promoted by S1P via S1PR-1 stimulation led to the development of pharmacological agents which are S1PR antagonists. These agents promote lymphocyte sequestration and reduce lymphocyte-driven inflammatory damage of the central nervous system (CNS) in animal models, encouraging their examination of efficacy in the treatment of multiple sclerosis (MS). Preclinical research has also demonstrated direct protective effects of S1PR antagonists within the CNS, by modulation of S1PRs, particularly S1PR-1 and S1PR-5, and possibly S1PR-2, independent of effects upon lymphocytes. Three of these agents, fingolimod, siponimod and ozanimod have been approved, and ponesimod has been submitted for regulatory approval. In patients with MS, these agents reduce relapse risk, sustained disability progression, magnetic resonance imaging markers of disease activity, and whole brain and/or cortical and deep gray matter atrophy. Future opportunities in the development of more selective and intracellular S1PR-driven downstream pathway modulators may expand the breadth of agents to treat MS.
Impulsivity and Empathy in Dating Violence among a Sample of College Females
Authors: Dodaj Arta , Sesar Kristina , Šimić Nataša
Published: July 2020

The predictive factors of violence between married couples or adolescents are well-known. However, less is known about the factors relating to intimate violence among college students. This study examined sociodemographic variables (age, duration of relationship, and relationship satisfaction), impulsivity, and empathy as predictors of dating violence, using data from 474 female college students from the University of Mostar, Bosnia and Herzegovina. The sample completed online the Conflict Tactics Scale 2 Short Form, the Interpersonal Reactivity Index, and the Short Impulsive Behavior Scale. The results indicated a higher prevalence of victimization than perpetration for psychological aggression. The obtained data showed that younger women and those in longer relationships, as well as those unsatisfied with their relationship, are more prone to experience psychological victimization or perpetration. Relationship satisfaction was also shown to be a predictor of physical perpetration causing injury. Impulsivity facets were found to have a differential weight in explaining dating violence. Empathy was shown to be a significant predictor of dating victimization, specifically “perspective taking” for psychological victimization and empathic concern for sexual victimization. These results suggest the need to develop specific interventions and prevention programs focused on relationship satisfaction, impulsivity, and empathy.
The Influence of a Surface Treatment of Metallic Titanium on the Photocatalytic Properties of TiO2 Nanotubes Grown by Anodic Oxidation
Authors: Marinko Živa , Suhadolnik Luka , Samardžija Zoran , Kovač Janez , Čeh Miran
Published: July 2020

Titanium dioxide (TiO2) nanotubes obtained by the anodic oxidation of titanium metal foils can be used for the photocatalytic degradation of organic pollutants. The aim of our study was to determine the influence of the titanium foil’s surface treatment on the final morphology of the TiO2 nanotubes and their photocatalytic activity. In our experiments, we used two different titanium foils that were electropolished or untreated prior to the anodic oxidation. The morphologies of the starting titanium foils and the resulting TiO2 nanotube layers were investigated and the photocatalytic activities measured by the decomposition of caffeine under UV irradiation. Our results showed that electropolishing of the starting foils produced a more uniform and smoother TiO2 nanotubes surface. In contrast, the TiO2 nanotube surfaces from untreated titanium foils mimic the initial surface roughness of the titanium foil. A comparison of the photocatalytic properties of the TiO2 nanotube layers obtained from the untreated and electropolished titanium foils showed that electropolishing does not necessarily improve the photocatalytic properties of the resulting TiO2 nanotube layer. It was found that the determining factors influencing the photocatalytic activity are the chemical impurities (Ti-nitride) on the surface of the titanium foils and the surface roughness of the TiO2 nanotube layer. The highest photocatalytic activity was achieved with the anodized untreated foil with the minimal presence of Ti-nitride and a relatively high roughness of the TiO2 nanotubes.
Robust Carbon-Stabilization of Few-Layer Black Phosphorus for Superior Oxygen Evolution Reaction
Authors: Zhang Mengjie , Zhu Wenchang , Yang Xingzhe , Feng Meng , Feng Hongbin
Published: July 2020

Few-layer exfoliated black phosphorus (Ex-BP) has attracted tremendous attention owing to its promising applications, including in electrocatalysis. However, it remains a challenge to directly use few-layer Ex-BP as oxygen-involved electrocatalyst because it is quite difficult to restrain structural degradation caused by spontaneous oxidation and keep it stable. Here, a robust carbon-stabilization strategy has been implemented to prepare carbon-coated Ex-BP/N-doped graphene nanosheet (Ex-BP/NGS@C) nanostructures at room temperature, which exhibit superior oxygen evolution reaction (OER) activity under alkaline conditions. Specifically, the as-synthesized Ex-BP/NGS@C hybrid presents a low overpotential of 257 mV at a current density of 10 mA cm−2 with a small Tafel slope of 52 mV dec−1 and shows high durability after long-term testing.
Collision Mechanism and Deposition Characteristics of Particles on the Catenary Insulator Surface
Authors: Zhao Shanpeng , Zhang Chenrui , Zhang Youpeng , Zhang Dingchang
Published: July 2020

To solve the problem of contamination flashover of a catenary insulator in an electrified railway, the collision mechanism and deposition characteristics of contamination particles on the insulator surface should be studied to ensure the safe operation of traction power supply systems. This research used horizontal and oblique cantilever insulators as objects, and established the collision model between particles and insulator surface under different arrangements. The deposition conditions of particles on the insulator surface were obtained, and the simulation model of insulator contamination accumulation was established by using the Euler two-phase flow. The difference of contamination deposition characteristics between horizontal and oblique cantilever insulators was analyzed with the volume fraction of contamination particles as the characterization parameter. By comparing the cantilever insulator with the positive feeder insulator, this study analyzed the influence of shed structure on the contamination deposition characteristics. Results show that the installation method of an insulator has an impact on the contamination deposition on the insulator surface. When particle size and wind velocity are fixed, the degree of contamination accumulation on the surface of the oblique cantilever insulator is constantly higher than that of the horizontal cantilever insulator. The insulator shed structure also has a certain effect on the contamination deposition on the insulator surface. For the cantilever insulator, the degree of contamination accumulation of the windward side is higher than that of the leeward side. However, for the positive feeder insulator, the degree of contamination accumulation of the windward side is consistently less than that of the leeward side. Measures to deal with contamination flashover of catenary insulator are proposed.
Genotyping by Sequencing Reads of 20 Vicia faba Lines with High and Low Vicine and Convicine Content
Authors: Heinrich Felix , Gültas Mehmet , Link Wolfgang , Schmitt Armin Otto
Published: July 2020

The grain faba bean (Vicia faba) which belongs to the family of the Leguminosae, is a crop that is grown worldwide for consumption by humans and livestock. Despite being a rich source of plant-based protein and various agro-ecological advantages its usage is limited due to its anti-nutrients in the form of the seed-compounds vicine and convicine (V+C). While markers for a low V+C content exist the underlying pathway and the responsible genes have remained unknown for a long time and only recently a possible pathway and enzyme were found. Genetic research into Vicia faba is difficult due to the lack of a reference genome and the near exclusivity of V+C to the species. Here, we present sequence reads obtained through genotyping-by-sequencing of 20 Vicia faba lines with varying V+C contents. For each line, ∼3 million 150 bp paired end reads are available. This data can be useful in the genomic research of Vicia faba in general and its V+C content in particular.
Learning to Teach: How a Simulated Learning Environment Can Connect Theory to Practice in General and Special Education Educator Preparation Programs
Authors: Landon-Hays Melanie , Peterson-Ahmad Maria B. , Frazier Andrea Dawn
Published: July 2020

Educator preparation programs have moved away from offering interest-based courses that prepare a teacher candidate on a more surface level and have opted to integrate more authentic experiences with technology that are infused into coursework. This research study focused on redesigning key courses in both the general and special education graduate-level educator preparation programs (EPPs) to infuse learning experiences through a simulated learning environment (Mursion) to help bridge teacher candidates’ coursework and field experiences, offering them robust experience with high leverage practices and technology that increases their own competency. Data from this study demonstrated that preservice teacher candidate work within the Mursion simulated learning environment increased use of high leverage practices related to strategic teaching, collaboration, differentiation, and providing feedback. Implications for instructional coaching, microteaching, repeated practice, and closing the research to practice gap are discussed.
Extended Segmented Beat Modulation Method for Cardiac Beat Classification and Electrocardiogram Denoising
Authors: Nasim Amnah , Sbrollini Agnese , Morettini Micaela , Burattini Laura
Published: July 2020

Beat classification and denoising are two challenging and fundamental operations when processing digital electrocardiograms (ECG). This paper proposes the extended segmented beat modulation method (ESBMM) as a tool for automatic beat classification and ECG denoising. ESBMM includes four main steps: (1) beat identification and segmentation into PQRS and TU segments; (2) wavelet-based time-frequency feature extraction; (3) convolutional neural network-based classification to discriminate among normal (N), supraventricular (S), and ventricular (V) beats; and (4) a template-based denoising procedure. ESBMM was tested using the MIT–BIH arrhythmia database available at Physionet. Overall, the classification accuracy was 91.5% while the positive predictive values were 92.8%, 95.6%, and 83.6%, for N, S, and V classes, respectively. The signal-to-noise ratio improvement after filtering was between 0.15 dB and 2.66 dB, with a median value equal to 0.99 dB, which is significantly higher than 0 (p < 0.05). Thus, ESBMM proved to be a reliable tool to classify cardiac beats into N, S, and V classes and to denoise ECG tracings.
Forging Common Origin in the Making of the Mexican Nation
Authors: Gutiérrez Chong Natividad
Published: July 2020

The Mexican nation was built by the state. This construction involved the formulation and dissemination of a national identity to forge a community that shares common culture and social cohesion. The focus of the article is to analyze the myth of the origin of the nation, mestizaje, as this is a long-lasting formula of national integration. After more than a century of mestizaje, real or fictitious, Indigenous and Afro-descendant peoples have begun to question the capability of this common origin since it invalidates the origins of many other ethnic communities, especially in the current phase of the nation state, which refers to the recognition of cultural diversity. The myth is propagated by official means and is highly perceived by society, due to its high symbolic content that is well reflected in popular pictorial representations. The final part of the article will refer to the mestizo myth in the imagination of some Indigenous intellectuals and students, who hold their own ethnic myths of foundation or origin.
Ground-Penetrating Radar Study of Progradational Units in Holocene Coastal Plains: Carchuna Beach (SE Spain)
Authors: Rey Javier , Martínez Julián , Hidalgo Mᵃ Carmen , Mendoza Rosendo , Campos Mᵃ José
Published: July 2020

The internal geometries of progradational units on the coastal plain in southeastern Spain (Carchuna Beach, Granada) since the maximum Holocene marine transgression (ca. 7000 cal BP) have been analysed using ground-penetrating radar (GPR). Radargram analysis shows the presence of sedimentary structures consistent with the progradation of coastal plains, interpreted as laminations associated with the foreset and topset. Laterally, five middle scale progradational units (H2, H3, H4, H5 and H6) are identified. Each of these units can be grouped into two major units. The general trend towards progradation is interrupted at the boundary of the units. Minor scale units (prograding wedges), deposited at topographically lower areas, are detected in GPR profiles. The radargrams also show channel erosional surfaces and sigmoidal landward dipping reflections interpreted as washover channel deposits, with the erosion and transference of sediments between the beach and the back-ridge domain. Furthermore, the saturation of coastline sediments with saltwater rapidly attenuates the GPR signal. Thus, the GPR is also a good tool to detect saltwater intrusions in shallow aquifers.
“The Ocean in Us”: Navigating the Blue Humanities and Diasporic Chamoru Poetry
Authors: Perez Craig Santos
Published: July 2020

This essay will explore the complex relationship between Pacific Islander Literature and the “Blue Humanities,” navigation traditions and canoe aesthetics, and Chamoru migration and diaspora. First, I will chart the history, theory, and praxis of Pacific voyaging traditions; the colonial history of restricting indigenous mobilities; and the decolonial acts of seafaring revitalization in the Pacific (with a specific focus on Guam). Then, I will examine the representation of seafaring and the ocean-going vessel (the canoe) as powerful symbols of Pacific migration and diasporic cultural identity in the context of what Elizabeth DeLoughrey termed, “narrative maritime legacies” (2007). Lastly, I will conduct a close-reading of the avant-garde poetry collection, A Bell Made of Stones (2013), by Chamoru writer Lehua Taitano. As I will show, Taitano writes about the ocean and navigation in order to address the history and traumas of Chamoru migration and diaspora. In terms of poetic form, I will argue that Taitano’s experimentation with typography and visual poetry embodies Chamoru outrigger design aesthetics and navigational techniques. In the end, I will show how a “Blue Humanities” approach to reading Pacific Islander literature highlights how the “New Oceania” is a profound space of Pacific migration and diasporic identity.
The Frontline Nurse’s Experience of Nursing Outlier Patients
Authors: Cheung Jasmine , West Sandra , Boughton Maureen
Published: July 2020

The frontline nurses’ experience of nursing with overstretched resources in acute care setting can affect their health and well-being. Little is known about the experience of registered nurses faced with the care of a patient outside their area of expertise. The aim of this paper is to explore the phenomenon of nursing the outlier patient, when patients are nursed in a ward that is not specifically developed to deal with the major clinical diagnosis involved (e.g., renal patient in gynecology ward). Using a hermeneutic phenomenological approach, eleven individual face-to-face in-depth interviews were conducted with registered nurses in New South Wales, Australia. The study identified that each nurse had a specialty construct developed from nursing in a specialized environment. Each nurse had normalized the experience of specialty nursing and had developed a way of thinking and practicing theorized as a “care ladder”. By grouping and analyzing various “care ladders” together, the nursing capacities common to nurses formed the phenomenological orientation, namely “the composite care ladder”. Compared to nursing specialty-appropriate patients, nursing the outlier patient caused disruption of the care ladder, with some nurses becoming less capable as they were nursing the outlier patient. Nursing the outlier patient disrupted the nurses’ normalized constructs of nursing. This study suggests that nursing patients in specialty-appropriate wards will improve patient outcomes and reduce impacts on the nurses’ morale.
Generating and Mapping Amazonian Urban Regions Using a Geospatial Approach
Authors: Cabrera-Barona Pablo F. , Bayón Manuel , Durán Gustavo , Bonilla Alejandra , Mejía Verónica
Published: July 2020

(1) background: Urban representations of the Amazon are urgently needed in order to better understand the complexity of urban processes in this area of the World. So far, limited work that represents Amazonian urban regions has been carried out. (2) methods: Our study area is the Ecuadorian Amazon. We performed a K-means algorithm using six urban indicators: Urban fractal dimension, number of paved streets, urban radiant intensity (luminosity), and distances to the closest new deforested areas, to oil pollution sources, and to mining pollution sources. We also carried out fieldwork to qualitatively validate our geospatial and statistical analyses. (3) results: We generated six Amazonian urban regions representing different urban configurations and processes of major cities, small cities, and emerging urban zones. The Amazonian urban regions generated represent the urban systems of the Ecuadorian Amazon at a general scale, and correspond to the urban realities at a local scale. (4) conclusions: An Amazonian urban region is understood as a set of urban zones that are dispersed and share common urban characteristics such a similar distance to oil pollution sources or similar urban radiant intensity. Our regionalization model represents the complexity of the Amazonian urban systems, and the applied methodology could be transferred to other Amazonian countries.
CRISPR/Cas9: A Robust Genome-Editing Tool with Versatile Functions and Endless Application
Authors: Zhang Baohong
Published: July 2020

Since a potential genome editing tool was first recognized in 2012 [...]
Mid-Gestation lethality of Atxn2l-Ablated Mice
Authors: Key Jana , Harter Patrick N. , Sen Nesli-Ece , Gradhand Elise , Auburger Georg , Gispert Suzana
Published: July 2020

Depletion of yeast/fly Ataxin-2 rescues TDP-43 overexpression toxicity. In mouse models of Amyotrophic Lateral Sclerosis via TDP-43 overexpression, depletion of its ortholog ATXN2 mitigated motor neuron degeneration and extended lifespan from 25 days to >300 days. There is another ortholog in mammals, named ATXN2L (Ataxin-2-like), which is almost uncharacterized but also functions in RNA surveillance at stress granules. We generated mice with Crispr/Cas9-mediated deletion of Atxn2l exons 5-8, studying homozygotes prenatally and heterozygotes during aging. Our novel findings indicate that ATXN2L absence triggers mid-gestational embryonic lethality, affecting female animals more strongly. Weight and development stages of homozygous mutants were reduced. Placenta phenotypes were not apparent, but brain histology showed lamination defects and apoptosis. Aged heterozygotes showed no locomotor deficits or weight loss over 12 months. Null mutants in vivo displayed compensatory efforts to maximize Atxn2l expression, which were prevented upon nutrient abundance in vitro. Mouse embryonal fibroblast cells revealed more multinucleated giant cells upon ATXN2L deficiency. In addition, in human neural cells, transcript levels of ATXN2L were induced upon starvation and glucose and amino acids exposure, but this induction was partially prevented by serum or low cholesterol administration. Neither ATXN2L depletion triggered dysregulation of ATXN2, nor a converse effect was observed. Overall, this essential role of ATXN2L for embryogenesis raises questions about its role in neurodegenerative diseases and neuroprotective therapies.
Aeroelastic Response of Suspended Pedestrian Bridges Made of Laminated Wood and Hemp
Authors: Rizzo Fabio
Published: July 2020

The work described in this paper investigated, by calculating critical flutter speed, the aeroelastic response of suspended pedestrian bridges made of a laminated wood structure and hemp cables and compared them to bridges with a steel structure and harmonic steel cables. Critical flutter speed was estimated using a numerical two degree of freedom (2-DOF) generalized deck model based on finite-element modal analysis. The critical flutter speeds of two sets of 25 different structural configurations, obtained by varying the deck chord and the permanent deck loads, made of steel and of laminated wood respectively, were estimated using experimental flutter derivatives obtained from 30 wind tunnel experiments. One of the most significant results was that pedestrian bridges made of laminated wood and hemp have a higher torsional frequency than those made of steel and that this affects critical flutter speed. A case study was performed and discussed by analyzing the structural and aeroelastic response of a 250 m pedestrian bridge with a 12 m deck chord and two approximately 32 m tall towers.
Uptake of Methylene Blue from Aqueous Solution by Pectin–Chitosan Binary Composites
Authors: Kong Dexu , Wilson Lee D.
Published: July 2020

To address the need to develop improved hybrid biopolymer composites, we report on the preparation of composites that contain chitosan and pectin biopolymers with tunable adsorption properties. Binary biopolymer composites were prepared at variable pectin–chitosan composition in a solvent directed synthesis, dimethyl sulfoxide (DMSO) versus water. The materials were characterized using complementary methods (infrared spectroscopy, thermal gravimetric analysis, pH at the point-of-zero charge, and dye-based adsorption isotherms). Pectin and chitosan composites prepared in DMSO yielded a covalent biopolymer framework (CBF), whereas a polyelectrolyte complex (PEC) was formed in water. The materials characterization provided support that cross-linking occurs between amine groups of chitosan and the –COOH groups of pectin. CBF-based composites had a greater uptake of methylene blue (MB) dye over the PEC-based composites. Composites prepared in DMSO were inferred to have secondary adsorption sites for enhanced MB uptake, as evidenced by a monolayer uptake capacity that exceeded the pectin–chitosan PECs by 1.5-fold. This work provides insight on the role of solvent-dependent cross-linking of pectin and chitosan biopolymers. Sonication-assisted reactions in DMSO favor CBFs, while cross-linking in water yields PECs. Herein, composites with tunable structures and variable physicochemical properties are demonstrated by their unique dye adsorption properties in aqueous media.
The Effect of Cold and Warm Anomalies on Phytoplankton Pigment Composition in Waters off the Northern Baja California Peninsula (México): 2007–2016
Authors: González-Silvera Adriana , Santamaría-del-Ángel Eduardo , Camacho-Ibar Víctor , López-Calderón Jorge , Santander-Cruz Jonatan , Mercado-Santana Alfredo
Published: July 2020

In this study, we report the response of phytoplankton community composition to cold and warm interannual events affecting the waters off the Baja California Peninsula from 2007 to 2016 based on data obtained from a single marine station (31.75° N/116.96° W). Included variables were satellite chlorophyll a, sea surface temperature (MODIS/Aqua), upwelling intensity, and field data (phytoplankton pigments, inorganic nutrients, light penetration). Phytoplankton pigments were determined by high performance liquid chromatography, and CHEMTAX software was used to determine the relative contributions of the main taxonomic groups to chlorophyll a. Our results confirm the decrease in phytoplankton biomass due to the influence of the recent Pacific Warm Anomaly (2014) and El Niño 2015–2016. However, this decrease was especially marked at the surface. When data from the entire water column was considered, this decrease was not significant, because at the subsurface Chla did not decrease as much. Nevertheless, significant changes in community composition occurred in the entire water column with Cyanobacteria (including Prochlorococcus) and Prymnesiophytes being dominant at the surface, while Chlorophytes and Prasinophytes made a strong contribution at the subsurface. Analysis of the spatial distribution of SST and satellite chlorophyll a made it possible to infer the spatial extension of these anomalies at a regional scale.
Physico-Chemical Characterization of Two Portuguese Coastal Systems: Ria de Alvor and Mira Estuary
Authors: Picado Ana , Mendes Joana , Ruela Rui , Pinheiro João , Dias João Miguel
Published: July 2020

The present study aims to research the physico-chemical processes in two under-researched coastal systems located on the west and south Portuguese coast—Mira Estuary and Ria de Alvor—through the development and exploitation of dedicated coupled physical and water quality models. Both systems are highly dynamic, supporting a wide range of biological diversity; however, they are characterized by distinct environmental and oceanographic conditions, enhancing the importance of a comparative approach. In this context, the Delft3D modeling suite was implemented and successfully calibrated and validated for both systems, accurately reproducing their hydrodynamic, hydrographic, and chemical features. A broad characterization of Mira Estuary and Ria de Alvor was carried out. Results show that the tidal wave interacts differently with the different geomorphology of each estuary. The tidal wave amplitude decreases as it propagates upstream for both estuaries; however, the magnitude for Ria de Alvor is higher. A flood tidal dominance was found for Mira Estuary, allowing the transport of well-oxygenated water into the estuary, contributing to high residence times in the middle estuary and therefore to poor dissolved oxygen (DO) replenishment. Ria de Alvor shifts from ebb dominance at the central area to flood dominance upstream with low residence times, allowing an effective exchange of water properties. Results also reveal that the water temperature is the dominant driver of seasonal dissolved oxygen variations in both estuaries, with the lowest levels occurring during the late summer months when the water temperature is highest. In addition, pH is influenced by biological activity and freshwater inflow.
Gap Analysis for DNA Barcode Reference Libraries for Aquatic Macroinvertebrate Species in the Apulia Region (Southeast of Italy)
Authors: Specchia Valeria , Tzafesta Eftychia , Marini Gabriele , Scarcella Salvatore , D’Attis Simona , Pinna Maurizio
Published: July 2020

The use of molecular tools (DNA barcoding and metabarcoding) for the identification of species and ecosystem biomonitoring is a promising innovative approach. The effectiveness of these tools is, however, highly dependent on the reliability and coverage of the DNA sequence reference libraries and it also depends on the identification of primer sets that work on the broadest range of taxa. In this study, a gap analysis of available DNA barcodes in the international libraries was conducted using the aquatic macroinvertebrate species checklist of the Apulia region in the southeast of Italy. Our analyses show that 42% of the 1546 examined species do not have representative DNA barcodes in the reference libraries, indicating the importance of working toward their completeness and addressing this effort toward specific taxonomic groups. We also analyzed the DNA barcode reference libraries for the primer set used to barcode species. Only for 52% of the examined barcoded species were the primers reported, indicating the importance of uploading this information in the databases for a more effective DNA barcode implementation effort and extensive use of the metabarcoding method. In this paper, a new combination of primers has revealed its experimental effectiveness at least on the species belonging to the three most represented taxa in the aquatic ecosystems of the Apulia region, highlighting the opportunity to develop combinations of primers useful at the regional level and the importance of studying DNA barcode gaps at the local/regional level. The DNA barcode coverage also varies among different taxonomic groups and aquatic ecosystem types in which a large number of species are rare. We tested the application of the DNA barcoding single species to a lagoon ecosystem (the lagoon named “Acquatina di Frigole” in the Apulia region) and we sampled two macroinvertebrate species lacking DNA barcodes from “Aquatina di Frigole” NATURA 2000 Site IT9150003, Fabulina fabula and Tritia nitida, generated two new CO1 barcodes and added them to a DNA barcode reference library.
Novel Perspective on Alzheimer’s Disease Treatment: Rosmarinic Acid Molecular Interplay with Copper(II) and Amyloid β
Authors: Kola Arian , Hecel Aleksandra , Lamponi Stefania , Valensin Daniela
Published: July 2020

Alzheimer’s disease is a severe disorder that affects millions of people worldwide. It is a very debilitating disease with no cure at the moment. The necessity of finding an effective treatment is very demanding, and the entire scientific community is putting in a lot of effort to address this issue. The major hallmark of Alzheimer’s disease is the presence of toxic aggregated species in the brain, impaired metal homeostasis, and high levels of oxidative stress. Rosmarinic acid is a well-known potent antioxidant molecule, the efficacy of which has been proved both in vitro and in vivo. In this study, we investigated the possible role played by rosmarinic acid as a mediator of the copper(II)-induced neurotoxicity. Several spectroscopic techniques and biological assays were applied to characterize the metal complexes and to evaluate the cytotoxicity and the mutagenicity of rosmarinic acid and its Cu(II) complex. Our data indicate that rosmarinic acid is able to interfere with the interaction between amyloid β and Cu(II) by forming an original ternary association.