bluuebunny/crossref_metadata_2025 huggingface dataset:
Cross comparison of alternative diagnostic protocols including substitution to the clinical sample, RNA extraction method and nucleic acid amplification technology for COVID-19 diagnosis
Authors: Segura-Ulate Ismael , Apú Navilla , Cortés Bernal , Querol-Audi Jordi , Zaldívar Yamitzel , Ortega Carlos Alexander , Flores-Mora Fernando , Gatica-Arias Andrés , Madrigal-Redondo Germán
Published: August 2024

Background

the gold-standard diagnostic protocol (GSDP) for COVID-19 consists of a nasopharyngeal swab (NPS) sample processed through traditional RNA extraction (TRE) and amplified with retrotranscription quantitative polymerase chain reaction (RT-qPCR). Multiple alternatives were developed to decrease time/cost of GSDP, including alternative clinical samples, RNA extraction methods and nucleic acid amplification. Thus, we carried out a cross comparison of various alternatives methods against GSDP and each other.

Methods

we tested alternative diagnostic methods using saliva, heat-induced RNA release (HIRR) and a colorimetric retrotranscription loop-mediated isothermal amplification (RT-LAMP) as substitutions to the GSDP.

Results

RT-LAMP using NPS processed by TRE showed high sensitivity (96%) and specificity (97%), closely matching GSDP. When saliva was processed by TRE and amplified with both RT-LAMP and RT-qPCR, RT-LAMP yielded high diagnostic parameters (88%–96% sensitivity and 95%–100% specificity) compared to RT-qPCR. Nonetheless, when saliva processed by TRE and detected by RT-LAMP was compared against the GSDP, the resulting diagnostic values for sensitivity (78%) and specificity (87%) were somewhat high but still short of those of the GSDP. Finally, saliva processed with HIRR and detected via RT-LAMP was the simplest and fastest method, but its sensitivity against GSDP was too low (56%) for any clinical application. Also, in this last method, the acidity of a large percentage of saliva samples (9%–22%) affected the pH-sensitive colorimetric indicator used in the test, requiring the exclusion of these acidic samples or an extra step for pH correction.

Discussion

our comparison shows that RT-LAMP technology has diagnostic performance on par with RT-qPCR; likewise, saliva offers the same diagnostic functionality as NPS when subjected to a TRE method. Nonetheless, use of direct saliva after a HIRR and detected with RT-LAMP does not produce an acceptable diagnostic performance.
Predicting radiofrequency thermocoagulation surgical outcomes in refractory focal epilepsy patients using functional coupled neural mass model
Authors: Cai Tianxin , Lin Yaoxin , Wang Guofu , Luo Jie
Published: August 2024

Objective

The success rate of achieving seizure freedom after radiofrequency thermocoagulation surgery for patients with refractory focal epilepsy is about 20–40%. This study aims to enhance the prediction of surgical outcomes based on preoperative decisions through network model simulation, providing a reference for clinicians to validate and optimize surgical plans.

Methods

Twelve patients with epilepsy who underwent radiofrequency thermocoagulation were retrospectively reviewed in this study. A coupled model based on model subsets of the neural mass model was constructed by calculating partial directed coherence as the coupling matrix from stereoelectroencephalography (SEEG) signals. Multi-channel time-varying model parameters of excitation and inhibitions were identified by fitting the real SEEG signals with the coupled model. Further incorporating these model parameters, the coupled model virtually removed contacts destroyed in radiofrequency thermocoagulation or selected randomly. Subsequently, the coupled model after virtual surgery was simulated.

Results

The identified excitatory and inhibitory parameters showed significant difference before and after seizure onset (p < 0.05), and the trends of parameter changes aligned with the seizure process. Additionally, excitatory parameters of epileptogenic contacts were higher than that of non-epileptogenic contacts, and opposite findings were noticed for inhibitory parameters. The simulated signals of postoperative models to predict surgical outcomes yielded an area under the curve (AUC) of 83.33% and an accuracy of 91.67%.

Conclusion

The multi-channel coupled model proposed in this study with physiological characteristics showed a desirable performance for preoperatively predicting patients’ prognoses.
Single-domain magnetic particles with motion behavior under electromagnetic AC and DC fields are a fatal cargo in Metropolitan Mexico City pediatric and young adult early Alzheimer, Parkinson, frontotemporal lobar degeneration and amyotrophic lateral sclerosis and in ALS patients
Authors: Calderón-Garcidueñas Lilian , Cejudo-Ruiz Fredy Rubén , Stommel Elijah W. , González-Maciel Angélica , Reynoso-Robles Rafael , Torres-Jardón Ricardo , Tehuacanero-Cuapa Samuel , Rodríguez-Gómez Arturo , Bautista Francisco , Goguitchaichvili Avto , Pérez-Guille Beatriz E. , Soriano-Rosales Rosa Eugenia , Koseoglu Emel , Mukherjee Partha S.
Published: August 2024

Metropolitan Mexico City (MMC) children and young adults exhibit overlapping Alzheimer and Parkinsons’ diseases (AD, PD) and TAR DNA-binding protein 43 pathology with magnetic ultrafine particulate matter (UFPM) and industrial nanoparticles (NPs). We studied magnetophoresis, electron microscopy and energy-dispersive X-ray spectrometry in 203 brain samples from 14 children, 27 adults, and 27 ALS cases/controls. Saturation isothermal remanent magnetization (SIRM), capturing magnetically unstable FeNPs ̴ 20nm, was higher in caudate, thalamus, hippocampus, putamen, and motor regions with subcortical vs. cortical higher SIRM in MMC ≤ 40y. Motion behavior was associated with magnetic exposures 25–100 mT and children exhibited IRM saturated curves at 50–300 mT associated to change in NPs position and/or orientation in situ. Targeted magnetic profiles moving under AC/AD magnetic fields could distinguish ALS vs. controls. Motor neuron magnetic NPs accumulation potentially interferes with action potentials, ion channels, nuclear pores and enhances the membrane insertion process when coated with lipopolysaccharides. TEM and EDX showed 7–20 nm NP Fe, Ti, Co, Ni, V, Hg, W, Al, Zn, Ag, Si, S, Br, Ce, La, and Pr in abnormal neural and vascular organelles. Brain accumulation of magnetic unstable particles start in childhood and cytotoxic, hyperthermia, free radical formation, and NPs motion associated to 30–50 μT (DC magnetic fields) are critical given ubiquitous electric and magnetic fields exposures could induce motion behavior and neural damage. Magnetic UFPM/NPs are a fatal brain cargo in children’s brains, and a preventable AD, PD, FTLD, ALS environmental threat. Billions of people are at risk. We are clearly poisoning ourselves.
Ptbp2 re-expression rescues axon growth defects in Smn-deficient motoneurons
Authors: Salehi Saeede , Zare Abdolhossein , Gandhi Gayatri , Sendtner Michael , Briese Michael
Published: August 2024

Spinal muscular atrophy (SMA) is a neuromuscular disorder caused by mutations or deletions in the survival motoneuron 1 (SMN1) gene, resulting in deficiency of the SMN protein that is essential for motoneuron function. Smn depletion in mice disturbs axonal RNA transport and translation, thereby contributing to axon growth impairment, muscle denervation, and motoneuron degeneration. However, the mechanisms whereby Smn loss causes axonal defects remain unclear. RNA localization and translation in axons are controlled by RNA-binding proteins (RBP) and we recently observed that the neuronal RBP Ptbp2 modulates axon growth in motoneurons. Here, we identify Smn as an interactor of Ptbp2 in the cytosolic compartments of motoneurons. We show that the expression level of Ptbp2 is reduced in axons but not in the somata of Smn-depleted motoneurons. This is accompanied by reduced synthesis of the RBP hnRNP R in axons. Re-expression of Ptbp2 in axons compensates for the deficiency of Smn and rescues the defects in axon elongation and growth cone maturation observed in Smn-deficient motoneurons. Our data suggest that Ptbp2 and Smn are components of cytosolic mRNP particles, contributing to the precise spatial and temporal control of protein synthesis within axons and axon terminals.
The impact of hunger on indulgent food choices is moderated by healthy eating concerns
Authors: Otterbring Tobias , Folwarczny Michał , Gasiorowska Agata
Published: August 2024

Research has shown that hungry individuals are more impulsive, impatient, and prone to make indulgent food choices compared to their satiated counterparts. However, the literature is still mixed, with some studies showing such results while others fail to demonstrate hunger effects on consumers’ choice behavior. The current cross-sectional study (N = 461) sought to address these inconsistencies by examining whether the link between hunger and people’s propensity to make indulgent (vs. virtuous) food choices is moderated by their healthy eating concerns. Our findings revealed a weak but significant association between participants’ self-reported hunger levels and their likelihood of making indulgent rather than virtuous food choices (e.g., preferring a chocolate cake instead of a fruit salad). Importantly, this effect was moderated by their healthy eating concerns, such that the link between hunger and choice likelihood of indulgent food options only emerged among participants who scored lower, but not higher, in healthy eating concerns. We also replicated these results in a robustness check that focused on the extent to which participants indicated having a healthy lifestyle (e.g., exercising regularly), with a similar moderating influence of this factor. Together, these findings shed light on the importance of considering certain boundary conditions for establishing a link between hunger and consumers’ food choices, thus adding nuance to the growing body of hunger-related literature. The results emphasize the importance of ensuring the availability of healthier snack options in environments wherein foods and beverages can be consumed, particularly at times when consumers tend to be hungry, to promote healthier eating habits.
Prospective nutrition-inflammation markers for predicting early stoma-related complications in patients with colorectal cancer undergoing enterostomy
Authors: Yuan Jie , Jiang Fan , Fu Xiaochao , Hou Yun , Hu Yali , Yang Qishun , Liu Liyang , Wang Yufu , Sheng Wangwang , Cao Fuao , He Jinghu , Chen Guanglei , Peng Cheng , Jiang Wei
Published: August 2024

Background

Enterostomy is important for radical resection of colorectal cancer (CRC). Nevertheless, the notable occurrence of complications linked to enterostomy results in a reduction in patients’ quality of life and impedes adjuvant therapy. This study sought to forecast early stoma-related complications (ESRCs) by leveraging easily accessible nutrition-inflammation markers in CRC patients.

Methods

This study involved 470 individuals with colorectal cancer who underwent intestinal ostomy at Changhai Hospital Affiliated with Naval Medical University as the internal cohort. Between January 2016 and December 2018, the patients were enrolled and randomly allocated into a primary training group and a secondary validation group, with a ratio of 2:1 being upheld. The research encompassed collecting data on each patient’s clinical and pathological status, along with preoperative laboratory results. Independent risk factors were identified through Lasso regression and multivariate analysis, leading to the development of clinical models represented by a nomogram. The model’s utility was assessed using decision curve analysis, calibration curve, and ROC curve. The final model was validated using an external validation set of 179 individuals from January 2015 to December 2021.

Results

Among the internal cohort, stoma complications were observed in 93 cases. Multivariate regression analysis confirmed that age, stoma site, and elevated markers (Mon, NAR, and GLR) in conjunction with diminished markers (GLB and LMR) independently contributed to an increased risk of ESRCs. The clinical model was established based on these seven factors. The training, internal, and external validation groups exhibited ROC curve areas of 0.839, 0.812, and 0.793, respectively. The calibration curve showed good concordance among the forecasted model with real incidence of ostomy complications. The model displayed outstanding predictive capability and is deemed applicable in clinical settings, as evidenced by Decision Curve Analysis.

Conclusion

This study identified nutrition-inflammation markers (GLB, NAR, and GLR) in combination with demographic data as crucial predictors for forecasting ESRCs in colorectal cancer patients. A novel prognostic model was formulated and validated utilizing these markers.
Naringin attenuates inflammatory injury to the bovine endometrium by regulating the endoplasmic reticulum stress–PI3K/AKT–autophagy axis
Authors: Lu Zihao , Peng Qingyang , Hu Ruiting , Wang Yan , Fan Kewei , Zhang Tao
Published: August 2024

Background: Endometritis seriously affects maternal reproductive health and fertility. Natural compounds have the characteristics of high efficiency and low residue in disease treatment. We aimed to discover and reveal the pharmacological effects of naringin, which is widely present in food and plants, on endometritis.

Methods: Based on network pharmacology, the potential targets and pathways of naringin’s actions on endometritis were predicted. Animal in vivo experiments were conducted to examine the inflammatory response of lipopolysaccharides (LPSs) in uterine tissue and the therapeutic effect of naringin. An in vitro primary bovine endometrial epithelial cell inflammation and drug treatment model was constructed. The production of reactive oxygen species (ROS) was measured using DCFH-DA, and the effect of naringin on LPS-induced endometritis was evaluated using HE staining, real-time quantitative PCR, Western blot, and immunofluorescence staining methods.

Results: Naringin alleviated LPS-induced inflammatory injury and oxidative stress in the endometrium of mice and bovine endometrial epithelial cells (bEECs). Furthermore, in vitro studies were carried out to reveal the potential anti-inflammatory mechanisms of naringin based on network pharmacology. We found that naringin significantly inhibited LPS-stimulated endoplasmic reticulum stress (ERS)-related gene and protein expression, thus reducing the unfolded protein response (UPR). Furthermore, treatment of naringin attenuated the autophagic flux induced by ERS. In a further study, we observed that PI3K/AKT pathway inhibitors or ERS inducers partially reverse naringin’s inhibition of autophagy and cell apoptosis.

Conclusion: It is demonstrated that naringin suppresses autophagy by directly inhibiting the ERS-PI3K/AKT axis and exerting anti-inflammatory and antioxidant effects in endometritis. These findings provide novel insights into the pathogenesis of endometritis, highlighting potential therapeutic targets of traditional herbs and compounds.
Gan-jiang-ling-zhu decoction improves steatohepatitis by regulating gut microbiota-mediated 12-tridecenoic acid inhibition
Authors: Xu Ruohui , Wu Jiaxuan , Pan Jiashu , Zhang Shengan , Yang Yunuo , Zhang Li , Zhou Wenjun , Wu Na , Hu Dan , Ji Guang , Dang Yanqi
Published: August 2024

Introduction:Gan–jiang–ling–zhu (GJLZ) decoction is a classical traditional Chinese medicine prescription. Through invigorating yang, activating qi and dissipating dampness, GJLZ decoction is widely applied for the treatment of chronic digestive disease, including nonalcoholic fatty liver disease. However, efficacy and mechanism of GJLZ decoction behind nonalcoholic steatohepatitis (NASH) treatment remains unelucidated.

Methods: NASH was induced in mice, followed by treatment with GJLZ decoction. Various methods including hematoxylin-eosin, oil red O staining, and triglyceride analysis were employed to evaluate the treatment effects of GJLZ decoction on NASH. Gut microbiota, metabolomics, cell viability assays, immunofluorescence and Western blotting were performed to unveil the mechanism behind GJLZ decoction.

Results: GJLZ decoction treatment significantly improved hepatic steatosis in mice with NASH. It led to remodeling of gut flora and metabolite structures, including the 12-tridecenoic acid level. 12-Tridecenoic acid aggravated hepatic steatosis by promoting acetyl-coenzyme A carboxylase alpha (ACC) expression and inhibiting carnitine palmitoyltransferase 1A (CPT1A) expression. GJLZ decoction treatment reduced the 12-tridecenoic acid level, inhibited ACC activity and promoted CPT1A expression.

Conclusion: Our results demonstrated that 12-tridecenoic acid aggravated hepatic steatosis by affecting the ACC–CPT1A axis and GJLZ decoction treatment effectively reduced the 12-tridecenoic acid level and improved steatosis.
Unlocking play willingness: the dual pathways of curiosity drive and downward social comparison in game advertising
Authors: Zetian Dai , Juan Xue , Jiaxin Du , Hoe Tan Wee
Published: August 2024

The integrity and clarity of information have long been regarded as the cornerstones of advertising strategy. However, recent game advertising has taken a different approach. Specifically, incomplete game videos, especially those showcasing losing gameplay, are more likely to stimulate players’ interest compared to complete videos of winning gameplay. This study, through five experiments, uncovers a dual-pathway mechanism behind this phenomenon. Firstly, information gaps resulting from incomplete videos trigger curiosity drive, compelling viewers to seek more information and reinforcing their willingness to engage in gaming. Secondly, witnessing failures in game demonstrations activates components of downward social comparison and competitive motivation. These findings offer valuable insights into the complex dynamics of game advertising, shedding light on the effects of information gaps, curiosity, and social comparison. They provide valuable implications for advertising strategies within the gaming industry.
Opposing objective and subjective wellbeing outcomes within an environmentally vulnerable delta: a case study of Volta Delta, Ghana
Authors: Cannings Laurence , Hutton Craig W. , Sorichetta Alessandro , Nilsen Kristine
Published: August 2024

Introduction

Despite a growing interest in the measurement and conceptualization of wellbeing, the integration within sustainability research, and the understanding of how different wellbeing outcomes relate, is limited. Many studies focus on singular, often objectively measured, outcomes, without acknowledging the breadth of available measures. This approach can result in crucial subjective information, which can be explored to understand actors’ behaviors and responses, being omitted from research and policy. This study explores objective and subjective wellbeing outcomes, and how they relate, within an environmentally vulnerable context. Wellbeing and environmental services are intrinsically interlinked, therefore, appropriate policy solutions are required to address human needs and pressures on supporting ecosystems.

Methods

This paper uses binary logistic regression modelling, and qualitative participatory rural appraisal methods, to understand the environmental conditions, including climatic hazards and landscape characteristics, associated with households experiencing different objective/subjective wellbeing outcomes within Volta Delta, Ghana.

Results

The mixed method approach highlights a differing relationship between inland agricultural areas impacted by drought and erosion, and coastal/riverine, peri-urban landscapes exposed to flooding and salinization. Agricultural areas associate with “poor but happy” outcomes, whereas peri-urban landscapes associate with being “non-poor but unhappy.” Drawing on existing literature, and both quantitative and qualitative results, these varying outcomes are hypothesized to be driven by differences in livelihood vulnerability, relative comparisons to others, responses to climatic hazards, and individualistic/collective wellbeing conceptualizations.

Discussion

Our study concludes that environmental conditions influence objective and subjective wellbeing through different mechanisms. Sustainable development research should incorporate both objective and subjective measures when implementing and monitoring policy to more comprehensibly capture, and improve, wellbeing in environmentally vulnerable locations.
Meta-analysis of structural and functional brain abnormalities in early-onset schizophrenia
Authors: Wang Lu , Liu Ruishan , Liao Juan , Xiong Xin , Xia Linfeng , Wang Weiwei , Liu Junqi , Zhao Fulin , Zhuo Lihua , Li Hongwei
Published: August 2024

Background

Previous studies based on resting-state functional magnetic resonance imaging(rs-fMRI) and voxel-based morphometry (VBM) have demonstrated significant abnormalities in brain structure and resting-state functional brain activity in patients with early-onset schizophrenia (EOS), compared with healthy controls (HCs), and these alterations were closely related to the pathogenesis of EOS. However, previous studies suffer from the limitations of small sample sizes and high heterogeneity of results. Therefore, the present study aimed to effectively integrate previous studies to identify common and specific brain functional and structural abnormalities in patients with EOS.

Methods

The PubMed, Web of Science, Embase, Chinese National Knowledge Infrastructure (CNKI), and WanFang databases were systematically searched to identify publications on abnormalities in resting-state regional functional brain activity and gray matter volume (GMV) in patients with EOS. Then, we utilized the Seed-based d Mapping with Permutation of Subject Images (SDM-PSI) software to conduct a whole-brain voxel meta-analysis of VBM and rs-fMRI studies, respectively, and followed by multimodal overlapping on this basis to comprehensively identify brain structural and functional abnormalities in patients with EOS.

Results

A total of 27 original studies (28 datasets) were included in the present meta-analysis, including 12 studies (13 datasets) related to resting-state functional brain activity (496 EOS patients, 395 HCs) and 15 studies (15 datasets) related to GMV (458 EOS patients, 531 HCs). Overall, in the functional meta-analysis, patients with EOS showed significantly increased resting-state functional brain activity in the left middle frontal gyrus (extending to the triangular part of the left inferior frontal gyrus) and the right caudate nucleus. On the other hand, in the structural meta-analysis, patients with EOS showed significantly decreased GMV in the right superior temporal gyrus (extending to the right rolandic operculum), the right middle temporal gyrus, and the temporal pole (superior temporal gyrus).

Conclusion

This meta-analysis revealed that some regions in the EOS exhibited significant structural or functional abnormalities, such as the temporal gyri, prefrontal cortex, and striatum. These findings may help deepen our understanding of the underlying pathophysiological mechanisms of EOS and provide potential biomarkers for the diagnosis or treatment of EOS.
A motivational training program for secondary physical education teachers based on the circumplex model: a study protocol of a randomised controlled trial
Authors: García-Cazorla Javier , Sevil-Serrano Javier , García-González Luis , Abós Ángel
Published: August 2024

In most self-determination theory (SDT) research, improving (de)motivating teaching styles provides numerous benefits for students and teachers, although there is less evidence of the latter. Although the recent circumplex model provides a fine-grained picture of the different (de)motivating teaching styles (i.e., autonomy support, structure, control, and chaos) that physical education (PE) teachers can use in their lessons, no previous motivational training programs have been based on this model. Moreover, all SDT-training programs have been implemented through different group sessions, but individual sessions have not been delivered. This study outlines the protocol of a motivational training program, derived from the circumplex model, designed to enhance motivating teaching styles (and prevent or decrease demotivating teaching styles) among PE teachers. Consequently, this program seeks to improve motivational variables and influence (mal)adaptive outcomes in both teachers and students. A randomised controlled trial design with a mixed-method approach. At least 16 secondary PE teachers will be assigned to either an experimental group or a control group, together with some of their students. The training program comprises four face-to-face group sessions and two follow-up sessions (one individual and one group session). PE teachers will learn how to support autonomy and provide structure, as well as to be less controlling and chaotic towards students. Over approximately five months, teachers will implement these motivational strategies during their PE classes. Different (de)motivating teaching styles, motivational variables, and (mal)adaptive outcomes will be assessed in both PE teachers and their students at three distinct points: before the training program (T1), during the intervention (T2), and at the end of the intervention (T3). Additionally, two discussion groups involving all experimental PE teachers will be held (one following the training program and another at the end of the intervention). The results from this study could be useful for developing motivational training programs for in-service PE teachers.

Clinical trial registration: ClinicalTrials.gov, identifier [NTC06479369].
SONOICE! a Sonar–Voice dynamic user interface for assisting individuals with blindness and visual impairment in pinpointing elements in 2D tactile readers
Authors: Ramôa Gaspar , Schmidt Vincent , Schwarz Thorsten , Stiefelhagen Rainer , König Peter
Published: August 2024

Pinpointing elements on large tactile surfaces is challenging for individuals with blindness and visual impairment (BVI) seeking to access two-dimensional (2D) information. This is particularly evident when using 2D tactile readers, devices designed to provide 2D information using static tactile representations with audio explanations. Traditional pinpointing methods, such as sighted assistance and trial-and-error, are limited and inefficient, while alternative pinpointing user interfaces (UI) are still emerging and need advancement. To address these limitations, we develop three distinct navigation UIs using a user-centred design approach: Sonar (proximity-radar sonification), Voice (direct clock-system speech instructions), and Sonoice, a new method that combines elements of both. The navigation UIs were incorporated into the Tactonom Reader device to conduct a trial study with ten BVI participants. Our UIs exhibited superior performance and higher user satisfaction than the conventional trial-and-error approach, showcasing scalability to varied assistive technology and their effectiveness regardless of graphic complexity. The innovative Sonoice approach achieved the highest efficiency in pinpointing elements, but user satisfaction was highest with the Sonar approach. Surprisingly, participant preferences varied and did not always align with their most effective strategy, underscoring the importance of accommodating individual user preferences and contextual factors when choosing between the three UIs. While more extensive training may reveal further differences between these UIs, our results emphasise the significance of offering diverse options to meet user needs. Altogether, the results provide valuable insights for improving the functionality of 2D tactile readers, thereby contributing to the future development of accessible technology.
Repozytorium Laboratorium Efektywnej Komunikacji – polska literatura prostojęzyczna
Authors: Gromulski Mateusz
Published: August 2024

The growing popularity of the plain language movement in Poland has resulted in a number of publications devoted to the subject. They approach plain language from different perspectives; they also differ in character. In this paper, I present a list of publications devoted to plain language and a proposal for their classification. I have also applied statistical analysis to this collection. Owing to these procedures, I was able to determine the current research findings, as well as to identify areas that require further exploration. In this paper, I also put forward a proposal for a new layout of the repository of the University of Warsaw Laboratory for Effective Communication, which may become helpful both in research on plain language and in plain language didactics.
Screening and Evaluation of Biomechanical Properties and Morphological Characteristics of Peduncles in Foxtail Millet
Authors: Zhang Lili , Xing Guofang , Liu Zhenyu , Zhang Yanqing , Li Hongbo , Wang Yuanmeng , Lu Jiaxin , An Nan , Zhao Zhihong , Wang Zeyu , Han Yuanhuai , Cui Qingliang
Published: August 2024

Mechanized harvesting is a crucial step in the agricultural production of foxtail millet (Setaria italica), as its peduncles are susceptible to bending and breaking during the harvesting process, leading to yield losses and deterioration in grain quality. To evaluate the suitability of foxtail millet for mechanical harvesting, this study comprehensively analyzed the biomechanical properties of the peduncles and related biological morphological characteristics of 116 foxtail millet accessions, establishing a system for indicator screening and comprehensive evaluation. Using partial correlation analysis and R-type cluster analysis, four biomechanical and seven related morphological indices of the peduncle were screened from 22 candidate indicators, with their coefficient of variation ranging from 6% to 80%. The entropy method was used to assign weights to the selected indices, with biomechanical factors contributing 47.4%, peduncle morphology 20.2%, spike morphology 27.6%, and plant height 4.8%. The Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) and Rank-Sum Ratio (RSR) methods were applied to rank and grade the classification of the 116 foxtail millet varieties into four performance groups: Excellent (8 varieties), Good (50 varieties), Moderate (51 varieties), and Poor (7 varieties). This study provides a scientific basis for the selection and evaluation of foxtail millet varieties.
Type III Secretion Effectors of Xanthomonas oryzae pv. oryzicola: The Arsenal to Attack Equivalent Rice Defense for Invasion
Authors: Tan Nawei , Huang Yechao , Miao Weiguo , Zhang Qingxia , Wu Tao
Published: August 2024

Rice–Xanthomonas oryzae pv. oryzicola (Xoc) is one of the commonly used rice models of host–pathogen interactions. Xoc causes bacterial leaf streak (BLS) and has quarantine status. As a Gram-negative pathogen, Xoc usually employs type III secretion effectors (T3SEs), including transcription activator-like effectors (TALEs) and non-TALEs, to interfere with the innate immunity of rice. However, few major resistance genes corresponding to Xoc are found in rice cultivations; only Rxo1-AvrRxo1 and Xo1-TALEs interactions have been discovered in rice–Xoc. In this review, we focus on the role of the T3S system (T3SS) in Xoc virulence and consider the reported non-TALEs, including AvrRxo1, AvrBs2, XopN, XopC2, XopAP, and XopAK, as well as TALEs including Tal2g/Tal5d, Tal2h, Tal2a, Tal7, Tal10a, TalI, Tal2b, and Tal2c. Interestingly, AvrRxo1, XopC2, and XopAP disturb stomatal opening to promote infection through targeting diverse signaling pathways in rice. Otherwise, Tal2b and Tal2c, respectively, activate two rice salicylic acid (SA) hydroxylation genes to redundantly suppress the SA-mediated basal defense, and TalI, which has unknown targets, suppresses the SA signaling pathway in rice. In addition, other Xoc virulence factors are discussed. In conclusion, several T3SEs from Xoc interfere with similar defense pathways in rice to achieve invasion, providing an outlook for the control of this disease through manipulating the conserved pathways.
Detection Dogs Working in Hot Climates: The Influence on Thermoregulation and Fecal Consistency
Authors: Slotta-Bachmayr Leopold , Oyugi Antony , Mutoro Noreen , Burak Mary , Wykstra Mary
Published: August 2024

Body temperature is an important physiological parameter that influences the performance of working dogs. The main cooling mechanism in dogs is panting to support evaporative cooling, which reduces the dog’s ability to detect scents. In this study, we investigated the general body condition of four detection dogs searching for cheetah scats in a hot environment in northern Kenya. We evaluated the effect on the dog’s body temperature post-work in the short term (within hours) and long term (12–24 h). The fecal consistency and mean body temperature of the investigated dogs differed significantly between individuals but not between locations (moderate Nairobi and hot Samburu). On the morning after fieldwork, the dogs showed a significantly increased body temperature (37.9 ± 0.8 °C) compared to resting days (37.5 ± 2.2 °C). In the short term, on the first day of fieldwork, the dog’s body temperature (n = 2) decreased after 10 min of rest. On the second consecutive day of fieldwork, the 10-min recovery period was too short, and the body temperature did not decrease significantly. Our data showed that the use of detection dogs in hot conditions is possible and useful but requires increased attention to prevent heat-related illness.
Tanshinone IIA Inhibits the Endoplasmic Reticulum Stress-Induced Unfolded Protein Response by Activating the PPARα/FGF21 Axis to Ameliorate Nonalcoholic Steatohepatitis
Authors: Pi Dajin , Liang Zheng , Pan Jinyue , Zhen Jianwei , Zheng Chuiyang , Fan Wen , Song Qingliang , Pan Maoxing , Yang Qinhe , Zhang Yupei
Published: August 2024

Nonalcoholic steatohepatitis (NASH) is a critical stage in the progression of nonalcoholic fatty liver disease (NAFLD). Tanshinone IIA (TIIA) is a tanshinone extracted from Salvia miltiorrhiza; due to its powerful anti-inflammatory and antioxidant biological activities, it is commonly used for treating cardiovascular and hepatic diseases. A NASH model was established by feeding mice a methionine and choline-deficient (MCD) diet. Liver surface microblood flow scanning, biochemical examination, histopathological examination, cytokine analysis through ELISA, lipidomic analysis, transcriptomic analysis, and Western blot analysis were used to evaluate the therapeutic effect and mechanism of TIIA on NASH. The results showed that TIIA effectively reduced lipid accumulation, fibrosis, and inflammation and alleviated endoplasmic reticulum (ER) stress. Lipidomic analysis revealed that TIIA normalized liver phospholipid metabolism in NASH mice. A KEGG analysis of the transcriptome revealed that TIIA exerted its effect by regulating the PPAR signalling pathway, protein processing in the ER, and the NOD-like receptor signalling pathway. These results suggest that TIIA alleviates NASH by activating the PPARα/FGF21 axis to negatively regulate the ER stress-induced unfolded protein response (UPR).
Warm and Dry Compound Events in Poland
Authors: Wibig Joanna , Jędruszkiewicz Joanna
Published: August 2024

The aim of this paper was to characterize the warm and dry compound events (WD days) in Poland during the period of 1966–2023, focusing on assessing the frequency and intensity of such events and their spatial and temporal variability, as well as on the driving factors of warm and dry compound events. WD days are those days that have a maximum temperature equal to or higher than the 90th percentile and the precipitation on that day and the 14 preceding days are equal to or less than the 25 percentile. During 1966–2023, the frequency of WD days increased significantly, mainly in April, the summer months, and December. Higher temperatures favored the occurrence of WD days from March to November, but, in winter months, the heat did not favor the occurrence of WD days. The exception was December, when high temperatures in the first part of the analyzed period did not favor the occurrence of a dry day, whereas, in the second part, it did. The strongest influence on the frequency of WD days had the East Atlantic pattern, where air flowed over Poland from the southwest. Warm and humid air flowing from the Atlantic Ocean must overcome the mountain barrier; therefore, it flows to Poland as warm and dry air. From spring to autumn, the WD days were related to an increase in the geopotential height in central Eastern Europe, and, in the winter, they were related with blocking over the Balkans.
Evaluation of Seasonal Prediction of Extreme Wind Resource Potential over China Based on a Dynamic Prediction System SIDRI-ESS V1.0
Authors: Yan Zixiang , Li Jinxiao , Zhou Wen , Lin Zouxing , Zang Yuxin , Li Siyuan
Published: August 2024

Wind resources play a pivotal role in building sustainable energy systems, crucial for mitigating and adapting to climate change. With the increasing frequency of extreme events under global warming, effective prediction of extreme wind resource potential can improve the safety of wind farms and other infrastructure, while optimizing resource allocation and emergency response plans. In this study, we evaluate the seasonal prediction skill for summer extreme wind events over China using a 20-year hindcast dataset generated by a dynamical seamless prediction system designed by Shanghai Investigation, Design and Research Institute Co., Ltd. (Shanghai, China) (SIDRI-ESS V1.0). Firstly, the hindcast effectively simulates the spatial distribution of summer extreme wind speed thresholds, even though it tends to overestimate the thresholds in most regions. Secondly, high prediction skills, measured by temporal correlation coefficient (TCC) and normalized root mean square error (nRMSE), are observed in northeast China, central east China, southeast China, and the Tibetan Plateau (TCC is about 0.5 and the nRMSE is below 0.9 in these regions). The highest skills emerge in southeast China with a maximum TCC greater than 0.7, and effective prediction skill can extend up to a 5-month lead time. Ensemble prediction significantly enhances predictive skill and reduces uncertainty, with 24 ensemble members being sufficient to saturate TCC and 12–16 members for nRMSE in most key regions and lead times. Furthermore, we show that the prediction skill for extreme wind counts is strongly related to the prediction skill for summer mean wind speeds, particularly in southeast China. Overall, SIDRI-ESS V1.0 shows promising performance in predicting extreme winds and has great potential to provide services to the wind industry. It can effectively help to optimize wind farm operating strategies and improve power generation efficiency. However, further improvements are needed, particularly in areas where prediction skills for extreme winds are influenced by smaller-scale weather phenomena and areas with complex underlying surfaces and climate characteristics.
Sufficient Efficiency Criteria for New Classes of Non-Convex Optimization Models
Authors: Treanţă Savin , Alsalami Omar Mutab
Published: August 2024

In this paper, we introduce and study a new class of minimization models driven by multiple integrals as cost functionals. Concretely, we formulate and establish some sufficient efficiency criteria for a feasible point in the considered optimization problem. To this end, we introduce and define the concepts of (Γ,ψ)-invexity and generalized (Γ,ψ)-invexity for the involved real-valued controlled multiple integral-type functionals. More precisely, we extend the notion of (generalized) (Γ,ψ)-invexity to the multiple objective control models driven by multiple integral functionals. In addition, innovative proofs are considered for the principal results derived in the paper.
Uniform Stabilization and Asymptotic Behavior with a Lower Bound of the Maximal Existence Time of a Coupled System’s Semi-Linear Pseudo-Parabolic Equations
Authors: Liu Nian
Published: August 2024

This article discusses the initial boundary value problem for a class of coupled systems of semi-linear pseudo-parabolic equations on a bounded smooth domain. Global solutions with exponential decay and asymptotic behavior are obtained when the maximal existence time has a lower bound for both low and overcritical energy cases. A sharp condition linking these phenomena is derived, and it is demonstrated that global existence also applies to the case of the potential well family.
Comparative Evaluation of UV-Vis Spectroscopy-Based Approaches for Hemoglobin Quantification: Method Selection and Practical Insights
Authors: Coll-Satue Clara , Jansman Michelle Maria Theresia , Hosta-Rigau Leticia
Published: August 2024

The growing demand for effective alternatives to red blood cells (RBCs) has spurred significant research into hemoglobin (Hb)-based oxygen carriers (HBOCs). Accurate characterization of HBOCs—including Hb content, encapsulation efficiency, and yield—is crucial for ensuring effective oxygen delivery, economic viability, and the prevention of adverse effects caused by free Hb. However, the choice of quantification methods for HBOCs is often driven more by tradition than by a thorough assessment of available options. This study meticulously compares various UV-vis spectroscopy-based methods for Hb quantification, focusing on their efficacy in measuring Hb extracted from bovine RBCs across different concentration levels. The findings identify the sodium lauryl sulfate Hb method as the preferred choice due to its specificity, ease of use, cost-effectiveness, and safety, particularly when compared to cyanmethemoglobin-based methods. Additionally, the study discusses the suitability of these methods for HBOC characterization, emphasizing the importance of considering carrier components and potential interferences by analyzing the absorbance spectrum before selecting a method. Overall, this study provides valuable insights into the selection of accurate and reliable Hb quantification methods, which are essential for rigorous HBOC characterization and advancements in medical research.
Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats
Authors: Yamamoto Yasuko , Taniguchi Takazumi , Shimazaki Atsushi
Published: August 2024

Rotenone is a mitochondrial complex I inhibitor that causes retinal degeneration. A study of a rat model of rotenone-induced retinal degeneration suggested that this model is caused by indirect postsynaptic N-methyl-D-aspartate (NMDA) stimulation triggered by oxidative stress-mediated presynaptic intracellular calcium signaling. To elucidate the mechanisms by which rotenone causes axonal degeneration, we investigated morphological changes in optic nerves and the change in retinal ganglion cell (RGC) number in rats. Optic nerves and retinas were collected 3 and 7 days after the intravitreal injection of rotenone. The cross-sections of the optic nerves were subjected to a morphological analysis with axon quantification. The axons and somas of RGCs were analyzed immunohistochemically in retinal flatmounts. In the optic nerve, rotenone induced axonal swelling and degeneration with the incidence of reactive gliosis. Rotenone also significantly reduced axon numbers in the optic nerve. Furthermore, rotenone caused axonal thinning, fragmentation, and beading in RGCs on flatmounts and decreased the number of RGC soma. In conclusion, the intravitreal injection of rotenone in rats induced morphological abnormities with a reduced number of optic nerve axons and RGC axons when the RGC somas were degenerated. These findings help elucidate the pathogenesis of optic neuropathy induced by mitochondrial dysfunction.
Animal Models of Retinopathy of Prematurity: Advances and Metabolic Regulators
Authors: Maurya Meenakshi , Liu Chi-Hsiu , Bora Kiran , Kushwah Neetu , Pavlovich Madeline C. , Wang Zhongxiao , Chen Jing
Published: August 2024

Retinopathy of prematurity (ROP) is a primary cause of visual impairment and blindness in premature newborns, characterized by vascular abnormalities in the developing retina, with microvascular alteration, neovascularization, and in the most severe cases retinal detachment. To elucidate the pathophysiology and develop therapeutics for ROP, several pre-clinical experimental models of ROP were developed in different species. Among them, the oxygen-induced retinopathy (OIR) mouse model has gained the most popularity and critically contributed to our current understanding of pathological retinal angiogenesis and the discovery of potential anti-angiogenic therapies. A deeper comprehension of molecular regulators of OIR such as hypoxia-inducible growth factors including vascular endothelial growth factors as primary perpetrators and other new metabolic modulators such as lipids and amino acids influencing pathological retinal angiogenesis is also emerging, indicating possible targets for treatment strategies. This review delves into the historical progressions that gave rise to the modern OIR models with a focus on the mouse model. It also reviews the fundamental principles of OIR, recent advances in its automated assessment, and a selected summary of metabolic investigation enabled by OIR models including amino acid transport and metabolism.
New-Onset Chronic Musculoskeletal Pain Following COVID-19 Infection Fulfils the Fibromyalgia Clinical Syndrome Criteria: A Preliminary Study
Authors: Khoja Omar , Mulvey Matthew , Astill Sarah , Tan Ai Lyn , Sivan Manoj
Published: August 2024

New-onset chronic musculoskeletal (MSK) pain (>3 months duration) is a common symptom of post-COVID-19 syndrome (PCS). This study aimed to characterise new-onset chronic MSK pain in patients with PCS and its overlap with Fibromyalgia Syndrome (FMS). We enrolled patients with new-onset chronic MSK pain post-COVID-19 and assessed the nature of the pain and associated symptoms using the C19-YRS (Yorkshire Rehabilitation Scale). The FMS assessment was conducted as part of a standard clinical examination using the American College of Rheumatology (ACR) 2010 criteria: (1) Widespread Pain Index (WPI) ≥ 7 and symptoms severity (SS) score ≥ 5, or WPI between 3 and 6 and SS score ≥ 9, (2) symptoms consistent for at least 3 months, and (3) no alternative diagnosis. Of the eighteen patients (average age 49.6 (SD 11.8) years; BMI 31.7 (SD 8.6)), twelve were female. The average symptom duration was 27.9 (SD 6.97) months post-infection. Thirteen patients (72.2%) met the FMS criteria, with an average WPI score of 8.8 and an average SS score of 8.2, indicating a high level of pain and significant quality of life impacts. These findings support the hypothesis that FMS may develop as a long-term sequela of a viral infection, underscoring the need for further research into post-viral long-term conditions.
The Monocrotaline Rat Model of Right Heart Disease Induced by Pulmonary Artery Hypertension
Authors: Krstic Anna Maria , Jones Timothy L. M. , Power Amelia S. , Ward Marie-Louise
Published: August 2024

Pulmonary artery hypertension (PAH) is characterised by increased pulmonary vascular resistance (PVR) resulting in elevated pressure in the pulmonary artery supplying the pulmonary circulation. Disease of the right ventricle (RV) often manifests as a result of PAH placing excessive pressure on the right side of the heart. Although a relatively rare disease in humans, the impact of sustained PAH is severe, with poor outcomes even in treated individuals. As PAH develops, the blood flow is restricted through the pulmonary arteries and the right ventricle hypertrophies due to the increased strain of pumping blood through the pulmonary circulation. With time, RV hypertrophy progresses to right heart failure, impacting the supply of blood to the left ventricle and systemic circulation. Although right heart failure can currently be treated, it cannot be cured. There is therefore a need for more research into the physiological changes that cause the heart to fail under pressure overload. This review aims to evaluate the monocrotaline (MCT) rat model of PAH as a means of studying the cellular mechanisms associated with the development of RV hypertrophy and right heart failure.