bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification of R2R3-MYB Transcription Factors: Discovery of a “Dual-Function” Regulator of Gypenoside and Flavonol Biosynthesis in Gynostemma pentaphyllum
Authors: Huang Ding , Ming Ruhong , Xu Shiqiang , Yao Shaochang , Li Liangbo , Huang Rongshao , Tan Yong
Published: January 2022

The R2R3-MYB gene family participates in several plant physiological processes, especially the regulation of the biosynthesis of secondary metabolites. However, little is known about the functions of R2R3-MYB genes in Gynostemma pentaphyllum (G. pentaphyllum), a traditional Chinese medicinal herb that is an excellent source of gypenosides (a class of triterpenoid saponins) and flavonoids. In this study, a systematic genome-wide analysis of the R2R3-MYB gene family was performed using the recently sequenced G. pentaphyllum genome. In total, 87 R2R3-GpMYB genes were identified and subsequently divided into 32 subgroups based on phylogenetic analysis. The analysis was based on conserved exon–intron structures and motif compositions within the same subgroup. Collinearity analysis demonstrated that segmental duplication events were majorly responsible for the expansion of the R2R3-GpMYB gene family, and Ka/Ks analysis indicated that the majority of the duplicated R2R3-GpMYB genes underwent purifying selection. A combination of transcriptome analysis and quantitative reverse transcriptase-PCR (qRT-PCR) confirmed that Gynostemma pentaphyllum myeloblastosis 81 (GpMYB81) along with genes encoding gypenoside and flavonol biosynthetic enzymes exhibited similar expression patterns in different tissues and responses to methyl jasmonate (MeJA). Moreover, GpMYB81 could bind to the promoters of Gynostemma pentaphyllum farnesyl pyrophosphate synthase 1 (GpFPS1) and Gynostemma pentaphyllum chalcone synthase (GpCHS), the key structural genes of gypenoside and flavonol biosynthesis, respectively, and activate their expression. Altogether, this study highlights a novel transcriptional regulatory mechanism that suggests that GpMYB81 acts as a “dual-function” regulator of gypenoside and flavonol biosynthesis in G. pentaphyllum.
Evaluation of the Visually Impaired Experience of the Sound Environment in Urban Spaces
Authors: Zhang Sen , Zhang Ke , Zhang Meng , Liu Xiaoyang
Published: January 2022

Visually impaired people have unique perceptions of and usage requirements for various urban spaces. Therefore, understanding these perceptions can help create reasonable layouts and construct urban infrastructure. This study recruited 26 visually impaired volunteers to evaluate 24 sound environments regarding clarity, comfort, safety, vitality, and depression. This data was collected in seven different types of urban spaces. An independent sample non-parametric test was used to determine the significance of the differences between environmental evaluation results for each evaluation dimension and to summarize the compositions of sound and space elements in the positive and negative influence spaces. The results suggested that visually impaired people (1) feel comfort, safety, and clarity in parks, residential communities, and shopping streets; (2) have negative perceptions of vegetable markets, bus stops, hospitals, and urban departments; (3) feel anxious when traffic sounds, horn sounds, manhole cover sounds, and construction sounds occur; and (4) prefer spaces away from traffic, with fewer and slower vehicles, with a suitable space scale, and moderate crowd density. These results provide a reference for the future design of activity venues (i.e., residential communities, vegetable markets, bus stops, parks, shopping streets, hospitals, and urban functional departments) and the planning of accessibility systems for visually impaired urban residents.
Sexual Boundary Violations via Digital Media Among Students
Authors: Budde Juergen , Witz Christina , Böhm Maika
Published: January 2022

As digital media becomes more central to the lives of adolescents, it also becomes increasingly relevant for their sexual communication. Sexting as an important image-based digital medium provides opportunities for self-determined digital communication, but also carries specific risks for boundary violations. Accordingly, sexting is understood either as an everyday, or as risky and deviant behavior among adolescents. In the affectedness of boundary violations gender plays an important role. However, it is still unclear to what extent digital sexual communication restores stereotypical gender roles and restrictive sexuality norms or, alternatively, enables new spaces of possibility. In this sense, current research points to a desideratum regarding adolescents’ orientations toward sexting as a practice between spaces of possibility and boundary violations. This paper discusses the possibilities, but also the risks, of intimate digital communication among adolescents. The main question is, how adolescents themselves perceive sexting practices and how they position themselves between both spaces for possibility and for the exchange of unwanted sexual content. For this purpose, orientations toward normalities and gender of students are reconstructed. To answer these questions, twelve single-sex, group discussions were carried out with students aged 16 and 17 at five different secondary schools in northern Germany. A total of 20 boys and 22 girls took part. The group discussions were structured by a narrative generating guideline. The analysis draws its methodology from the Documentary Method, regarding implicit and explicit forms of knowledge and discourse. It results in a typology of three types with different orientations. The study shows, that most of the students consider sexting to be a risky practice; only one type shows normality in the use of sexting. At the same time, some of the young people are interested in experimenting with image-based intimate digital communication. Further, gender differences in use and affectedness are also documented. In this way, orientations toward gender stereotypes “favor” both the attribution of responsibility to girls, and overlook the responsibility of students who perpetrated the boundary violation. The orientations of adolescents should be taken more into account in research as well as in educational programs for the prevention of sexual violence.
Flow With Nature Treatment for Depression: Participants’ Experiences
Authors: Salonen Kirsi , Hyvönen Katriina , Paakkolanvaara Jane-Veera , Korpela Kalevi
Published: January 2022

This study examined Flow with Nature (FWN) treatment, which is an integrative intervention (rehabilitation) based on eco and environmental psychology, psychotherapeutic theories and professional psychological practice. FWN is intended for depression rehabilitation with the help of social support, nature environments and FWN exercises. Exercises encourage sensing the environment, mindful awareness, psychological processing and focusing on the future. The FWN treatment proceeds in separate stages (horizon, growth and path), which emphasise nature, group (social support) and FWN exercises differently. This study focused on the experiences of the participants in the FWN treatment. Finnish adults who had been diagnosed with clinical depression took part in the FWN treatment (N = 82) and answered feedback questions (by paper, electronic questionnaire or phone discussion). Answers were analysed using theory-based content analysis. Data were collected between spring 2019 and spring 2020. The majority of the participants were women (82%) and on average 44 years old. Content analysis revealed that the participant feedback answers were in agreement with the central theoretical themes of FWN. The participants emphasised the significance of nature, social support and exercises differently. Moreover, the significance of these ingredients differed according to the stages of treatment: in the horizon stage restorative (e.g., fascination) and comprehensive nature experiences (e.g., connectedness with nature), in the growth stage social support (e.g., peer support) and in the path stage environmental self-regulation (e.g., nature as a part of life) were emphasised. These results are in accordance with the objectives of the stages and seem to support the phase-based rationale. The participants’ experiences of the key elements of the intervention, social support and nature environment were experienced mainly positively, which supports their inclusion in future intervention versions. In the future, FWN exercises should be developed to better enable participants’ possibilities for concentration and being present in the moment (mindfulness). Exercises should also be simplified to match the target group’s cognitive abilities.
The Impact of Cooperative Learning on University Students’ Academic Goals
Authors: Mendo-Lázaro Santiago , León-del-Barco Benito , Polo-del-Río María-Isabel , López-Ramos Víctor M.
Published: January 2022

Cooperative learning encourages the development of interpersonal skills and motivates students to participate more actively in the teaching and learning process. This study explores the impact of cooperative learning on the academic goals influencing university students’ behavior and leading to the attainment of a series of academic objectives. To this end, a quasi-experimental pretest-posttest control group design was used, with a sample of 509 university students from Preschool, Primary and Social Education undergraduate degree courses. Using the Academic Goals Questionnaire (AGQ), pretest and posttest measures were taken via self-reports to evaluate three types of academic goals: learning goals, social reinforcement goals and achievement goals. The results show that cooperative learning is an effective tool for encouraging university students to develop academic goals that motivate them to fully engage with the tasks they are set in order to acquire knowledge and skills (learning goals). In addition, when students are asked to work as part of a team on an autonomous basis without the structure and supervision necessary to ensure a minimum standard of cooperation, they display a greater tendency toward social reinforcement goals than toward learning and achievement goals. These findings contribute new knowledge to the conceptual framework on cooperative learning. Goals may be considered one of the most important variables influencing students’ learning and the use of cooperative learning techniques in university classrooms creates the necessary conditions for encouraging students to develop goals oriented toward learning.
Impact of Induced Moods, Sensation Seeking, and Emotional Contagion on Economic Decisions Under Risk
Authors: Efimov Kirill , Ntoumanis Ioannis , Kuskova Olga , Kadieva Dzerassa , Panidi Ksenia , Kosonogov Vladimir , Kazanina Nina , Shestakova Anna , Klucharev Vasily , Jääskeläinen Iiro P.
Published: January 2022

In addition to probabilities of monetary gains and losses, personality traits, socio-economic factors, and specific contexts such as emotions and framing influence financial risk taking. Here, we investigated the effects of joyful, neutral, and sad mood states on participants’ risk-taking behaviour in a simple task with safe and risky options. We also analysed the effect of framing on risk taking. In different trials, a safe option was framed in terms of either financial gains or losses. Moreover, we investigated the effects of emotional contagion and sensation-seeking personality traits on risk taking in this task. We did not observe a significant effect of induced moods on risk taking. Sad mood resulted in a slight non-significant trend of risk aversion compared to a neutral mood. Our results partially replicate previous findings regarding the presence of the framing effect. As a novel finding, we observed that participants with a low emotional contagion score demonstrated increased risk aversion during a sad mood and a similar trend at the edge of significance was present in high sensation seekers. Overall, our results highlight the importance of taking into account personality traits of experimental participants in financial risk-taking studies.
Implementation of the DSM-5 and ICD-11 Dimensional Models of Maladaptive Personality Traits Into Pre-bariatric Assessment
Authors: Riegel Karel D. , Konecna Judita , Matoulek Martin , Rosova Livia
Published: January 2022

Background: Personality pathology does not have to be a contraindication to a bariatric surgery if a proper pre-surgical assessment is done. Indicating subgroups of patients with their specific needs could help tailor interventions and improve surgical treatment outcomes.

Objectives: Using the Alternative DSM-5 model for personality disorders (AMPD) and the ICD-11 model for PDs to detect subgroups of patients with obesity based on a specific constellation of maladaptive personality traits and the level of overall personality impairment.

Methods: 272 consecutively consented patients who underwent a standard pre-surgical psychological assessment. The majority were women (58.0%), age range was 22–79 years (M = 48.06, SD = 10.70). Patients’ average body mass index (BMI) was 43.95 kg/m2. All participants were administered the Personality Inventory for DSM-5 (PID-5) from which Level of Personality Functioning Scale-Self Report (LPFS-SR) and Standardized Assessment of Severity of Personality Disorder (SASPD) scores were gained using the “crosswalk” for common metric for self-reported severity of personality disorder. The k-means clustering method was used to define specific subgroups of patients with obesity and replicated for equality testing to the samples of non-clinical respondents and psychiatric patients.

Results: The cluster analysis detected specific groups in the sample of patients with obesity, which differed quantitatively from the samples of non-clinical respondents and psychiatric patients. A vast majority of patients with obesity showed above-average values in most of the PID-5 facets compared to the United States representative general community sample. In two out of the three clusters defined, patients demonstrated moderate (> M + 1.5 × SD) to severe (> M + 2.0 × SD) personality psychopathology within the Detachment and Negative Affectivity domains according to PID-5, which in one of the clusters corresponded to the mild overall impairment in both, LPFS-SR (M = 2.18, SD = 0.27) and SASPD (M = 8.44, SD = 2.38). Moreover, higher levels of psychopathology prove to be associated with higher age and use of psychiatric medication.

Conclusions: The dimensional DSM-5 and ICD-11 trait models are suitable procedures for defining specific “characters” of patients in a pre-bariatric setting. As such, they help to identify subgroups of patients with obesity who are different from general population and psychiatric patients. Implications for clinical practice and further research are discussed.
Hardiness in Family Caregivers During Caring From Persons With Alzheimer's Disease: A Deductive Content Analysis Study
Authors: Hosseini Lida , Sharif Nia Hamid , Ashghali Farahani Mansoureh
Published: January 2022

Objective: This study was designed to describe the experiences of family Caregivers' hardiness in caring for Alzheimer's Patients.

Methods: The deductive content analysis method was performed between April 2020 and February 2021 in one of the teaching hospitals in Iran. Fourteen family caregivers of Alzheimer's patients were selected using purposive and snowballing sampling and the data were collected by semi-structured interviews. After that, data were analyzed using Elo and Kingas steps.

Results: The results of this study showed that based on the experiences of family caregivers, the family caregivers' hardiness in caring for Alzheimer's patients is a feature of cognitive ability to deal with stressful care situations and consists of five dimensions of commitment, control, challenge, communication and culture with 22 generic categories that they were nested into this five dimension.

Conclusion: Family caregivers' hardiness is a trait related to the individual and environmental factors, and the prevailing social and cultural conditions affect the individual's perception and experience of hardship and threats, as well as his/her understanding of protective factors and how to use them. Therefore, hardiness should not be interpreted as a simple approach regardless of culture.
Psychometric Properties of the QoL-ME: A Visual and Personalized Quality of Life Assessment App for People With Severe Mental Health Problems
Authors: Buitenweg David C. , van de Mheen Dike , van Oers Hans A. M. , van Nieuwenhuizen Chijs
Published: January 2022

Background: Quality of Life (QoL) assessment in people with severe mental health problems may benefit from improved personalization and accessibility. Therefore, an innovative, digital, visual, and personalized QoL assessment app for people with severe mental health problems was recently developed: the QoL-ME. The aim of this study was to evaluate the psychometric quality of the QoL-ME by assessing its reliability, validity, and responsiveness.

Methods: To examine the reliability of the QoL-ME, the internal consistency of its subscales was assessed using Cronbach's Alpha. Correlations between the QoL-ME and the MANSA were computed to appraise the construct validity of the QoL-ME. Internal responsiveness was evaluated using the standardized response mean and external responsiveness was investigated using hierarchical regression.

Results: Cronbach's Alpha's of the subscales of the QoL-ME ranged between 0.5 and 0.84. In accordance with expectations, the language-based core version of the QoL-ME correlated strongly (r = between 0.55 and 0.76) with the MANSA, whilst the picture-based additional modules of the QoL-ME correlated moderately (r = 0.3) with the MANSA. The standardized response mean was 0.23 and the regression model revealed a coefficient β of −0.01.

Conclusions: The QoL-ME has adequate psychometric properties. In comparison with similar pictorial instruments, both the QoL-ME's reliability and validity can be considered as sufficient. The results indicate that the responsiveness of the QoL-ME is insufficient. Additional research is needed to evaluate and potentially modify the instrument to improve its responsiveness.
Interactions Between Opioids and Dextroamphetamine on Locomotor Activity: Influence of an Opioid's Relative Efficacy at the Mu Receptor
Authors: Smith Mark A. , Ballard Shannon L. , Ballesteros Clarise F. , Bonge Samantha A. , Casimir Alexander T. , Childs Lauren M. , Feinstein Max A. , Griffith Annie K. , Johansen Alexandra N. , Lee Daegeon , Mauser A. Caroline , Moses Cassidy M. , Robertson Ian J. , Robles Javier U. , Strickland Justin C. , Walters Mary E. , Yoo Seeley J.
Published: January 2022

Opioids and stimulants are often used in combination for both recreational and non-recreational purposes. High-efficacy mu opioid agonists generally increase the behavioral effects of stimulants, whereas opioid receptor antagonists generally attenuate the behavioral effects of stimulants; however, less is known regarding the interactions between stimulants and opioids possessing low to intermediate efficacy at the mu receptor. The purpose of this study was to examine the role of an opioid's relative efficacy at the mu receptor in altering the behavioral effects of dextro(d-)amphetamine. To this end, opioids possessing a range of relative efficacy at the mu receptor were examined alone and in combination with cumulative doses of d-amphetamine on a test of open-field, locomotor activity in male rats. Levorphanol, buprenorphine, butorphanol, nalbuphine, (-)-pentazocine, (-)-metazocine, (-)-cyclazocine, (-)-NANM, and nalorphine increased the locomotor effects of d-amphetamine in either an additive or greater-than-additive manner according to an effect-additive model. Only the selective, high-efficacy kappa agonist, spiradoline, and the non-selective opioid receptor antagonist, naloxone, failed to increase the effects of d-amphetamine under the conditions examined. These data indicate that opioids possessing a large range of relative efficacy at the mu receptor, including those possessing very low relative efficacy, significantly increase the locomotor effects of d-amphetamine.
The Terms of “You(s)”: How the Term of Address Used by Conversational Agents Influences User Evaluations in French and German Linguaculture
Authors: Ollier Joseph , Nißen Marcia , von Wangenheim Florian
Published: January 2022

Background: Conversational agents (CAs) are a novel approach to delivering digital health interventions. In human interactions, terms of address often change depending on the context or relationship between interlocutors. In many languages, this encompasses T/V distinction—formal and informal forms of the second-person pronoun “You”—that conveys different levels of familiarity. Yet, few research articles have examined whether CAs' use of T/V distinction across language contexts affects users' evaluations of digital health applications.

Methods: In an online experiment (N = 284), we manipulated a public health CA prototype to use either informal or formal T/V distinction forms in French (“tu” vs. “vous”) and German (“du” vs. “Sie”) language settings. A MANCOVA and post-hoc tests were performed to examine the effects of the independent variables (i.e., T/V distinction and Language) and the moderating role of users' demographic profile (i.e., Age and Gender) on eleven user evaluation variables. These were related to four themes: (i) Sociability, (ii) CA-User Collaboration, (iii) Service Evaluation, and (iv) Behavioral Intentions.

Results: Results showed a four-way interaction between T/V Distinction, Language, Age, and Gender, influencing user evaluations across all outcome themes. For French speakers, when the informal “T form” (“Tu”) was used, higher user evaluation scores were generated for younger women and older men (e.g., the CA felt more humanlike or individuals were more likely to recommend the CA), whereas when the formal “V form” (“Vous”) was used, higher user evaluation scores were generated for younger men and older women. For German speakers, when the informal T form (“Du”) was used, younger users' evaluations were comparable regardless of Gender, however, as individuals' Age increased, the use of “Du” resulted in lower user evaluation scores, with this effect more pronounced in men. When using the formal V form (“Sie”), user evaluation scores were relatively stable, regardless of Gender, and only increasing slightly with Age.

Conclusions: Results highlight how user CA evaluations vary based on the T/V distinction used and language setting, however, that even within a culturally homogenous language group, evaluations vary based on user demographics, thus highlighting the importance of personalizing CA language.
Effects of Saliva From Periodontally Healthy and Diseased Subjects on Barrier Function and the Inflammatory Response in in vitro Models of the Oral Epithelium
Authors: Roy Antoine , Ben Lagha Amel , Gonçalves Reginaldo , Grenier Daniel
Published: January 2022

Background: Periodontitis is a multifactorial, bacteria-mediated chronic inflammatory disease that results in the progressive destruction of the tooth-supporting tissues. It is well-known that saliva from subjects suffering from this disease generally contains higher levels of pro-inflammatory mediators, matrix metalloproteinases (MMP), and bacteria-derived toxic products. The aim of this study was to investigate and compare the effects of saliva from periodontally healthy and diseased subjects on the barrier function and inflammatory response in in vitro models of the oral epithelium.

Methods: Unstimulated saliva samples from two groups of subjects, one with a healthy periodontium (n = 12) and one with severe generalized periodontitis (n = 11), were filter-sterilized. All the saliva samples were analyzed using an immunological multiplex assay to determine the levels of various cytokines and MMPs relevant to periodontitis. The impact of saliva on epithelial barrier integrity was assessed by monitoring transepithelial electrical resistance (TER) in an oral epithelium model using the B11 keratinocyte cell line. GMSM-K oral epithelial cells were treated with saliva from both groups to determine their ability to induce the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8), as determined by an enzyme-linked immunosorbent assay (ELISA).

Results: Saliva from the periodontitis subjects contained significantly higher concentrations of matrix metalloproteinase-8 (MMP-8), matrix metalloproteinase-9 (MMP-9), IL-8, and C-X-C motif chemokine ligand 1 (CXCL1) compared to saliva from the healthy subjects. Saliva from the healthy and periodontitis subjects affected cytokine secretion and TER in a similar manner. More specifically, saliva from both groups increased TER and induced IL-6 and IL-8 secretion in the in vitro oral epithelium models used.

Conclusion: Independently of the presence or absence of periodontitis, saliva can increase the relative TER and the secretion of IL-6 and IL-8 in in vitro models of the oral epithelium.
Re-purposing Universities: The Path to Purpose
Authors: Hurth Victoria , Stewart Iain S.
Published: January 2022

As the extent of damage to environmental systems from our business-as-usual activity becomes ever more alarming, Universities as core social institutions are under pressure to help society lead the transition to a sustainable future. Their response to the issues, that they themselves have helped reveal, has, however, been widely criticised for being wholly inadequate. Universities can be observed to engage with sustainability issues inad-hocways, with the scale of attention and commitment dependant mainly on the level of pressure exerted by stakeholders that works to overcome aspects of inherent inertia. Sustainability initiatives can therefore be regarded mainly as bolt-ons. This mirrors how other sectors, including businesses, have tended to respond. As the environmental and social crisis mounts and the window for adaptive change to ensure long-term wellbeing for all narrows, the pressure for deeper systemic change builds. It is in this context that transformation to a “purpose-driven organisation” has emerged as a systemic approach to change, enabling an organisation to align deeply and rapidly with society's long-term best interest and hence a sustainable future. Nowhere has this concept been taken forward more obviously than in the business sector. As business leadership towards purpose becomes more apparent, so the lack of action in this area by universities appears starker. In this paper we clarify what it means to be a purpose-driven organisation, why and how it represents a deep holistic response to unsustainability, and what core questions emerging from the business world university leaders can ask themselves to begin the practical journey to transform their institutions into purpose-driven universities.
Development and Feasibility Assessment of Virtual Reality-Based Relaxation Self-Training Program
Authors: Jeong Hyu Seok , Oh Jooyoung , Paik Minjeong , Kim Hyunji , Jang Sooah , Kim Beom Soo , Kim Jae-Jin
Published: January 2022

Diaphragmatic breathing and progressive muscle relaxation (PMR) are an effective way for relaxation training and anxiety control, but their use is not common to the general public. Today, as the need for non-face-to-face contact increases, virtual reality (VR)-based self-training is gaining attention in public health. This study aimed to evaluate the feasibility of the newly developed VR-based relaxation training program. Both diaphragmatic breathing and PMR can be trained without an assistant using this VR application in three steps: 1) learning in a virtual clinic, 2) review in a comfortable virtual environment, and 3) practice in outdoor virtual environments. Self-training is recommended on a 3-weeks schedule with a total of 4–6 trials per day for 4 days a week. Thirty-one healthy volunteers were divided into the VR (n = 15) and worksheet (n = 16) groups, and participated in self-training under similar conditions as much as possible. Multiple evaluations were performed before, during, and after self-training. The change rates of all psychological and psychophysiological measures before and after self-training did not significantly differ between the two groups. The levels of tension after breathing practices showed no group difference, whereas those after PMR practices were significantly lower in the VR group than in the worksheet group. In the VR group, trials of outdoor practices tended to induce a decrease of the tension level, particularly after outdoor breathing trials. The VR group gave a practicable score of 70 points or more, average 43.5, and average 180.3 for usability, cybersickness, and presence of this program, respectively. These results suggest that the VR-based relaxation self-training program can be used by healthy people as a means of relaxation. In the use of this program, diaphragmatic breathing may be used more easily, but the benefit of using VR is higher in PMR. These findings provide justification for a randomized controlled study of whether this program can be used for stress relief in the general population and, furthermore, treatment of patients with anxiety disorders.
COVID-19 and Work–Family Conflicts in Germany: Risks and Chances Across Gender and Parenthood
Authors: Reimann Mareike , Peters Eileen , Diewald Martin
Published: January 2022

The COVID-19 pandemic has dramatically affected everyone’s daily life in one way or another, requiring a re-negotiation of existing strategies for work–life integration, not only for individuals but also within families and partnerships. To contribute to existing knowledge on work-life integration during COVID-19 in Germany, we look at gender and parenthood differences in the experiences of work-to-family (WFC) and family-to-work (FWC) conflicts. By accounting for employees’ previous conflict experiences, we were able to reveal the extent to which the current conditions contributed to differences in these conflicts. Moreover, we explored the relevance of demands and resources in the family and work spheres as a way to explain different levels of WFC and FWC across gender and parenthood. Our analyses are based on a sample of 660 employees from a German linked employer–employee panel study and a COVID-19 follow-up survey conducted in late 2020. Results revealed that work–family conflict experiences before the pandemic play an important role in current conflict perceptions. Whereas WFC were more likely to be accentuated during the pandemic, prior FWC experiences may have helped to mitigate conflicts under these new conditions. Work–family conflicts in general have increased during the COVID-19 pandemic, but this finding applied only to conflicts in the family-to-work direction. Although such increases were not limited to parents, they were particularly high in this group. Overall, gender differences in work–family conflicts were absent, but differences were found between mothers and fathers. The need to compensate for a lack of external childcare, as well as having to work from home, increased FWC, especially among fathers. This study suggests that FWC in particular became more important during the pandemic; however, parents were not the only ones who were disadvantaged when it came to work–life integration; childless individuals likewise struggled to balance the demands of work and private life.
The Relationship Between the Distribution of Training Intensity and Performance of Kayak and Canoe Sprinters: A Retrospective Observational Analysis of One Season of Competition
Authors: Matzka Manuel , Leppich Robert , Holmberg Hans-Christer , Sperlich Billy , Zinner Christoph
Published: January 2022

Purpose:To evaluate retrospectively the training intensity distribution (TID) among highly trained canoe sprinters during a single season and to relate TID to changes in performance.

Methods:The heart rates during on-water training by 11 German sprint kayakers (7 women, 4 men) and one male canoeist were monitored during preparation periods (PP) 1 and 2, as well as during the period of competition (CP) (total monitoring period: 37 weeks). The zones of training intensity (Z) were defined as Z1 [<80% of peak oxygen consumption (VO2peak)], Z2 (81–87% VO2peak) and Z3 (>87% VO2peak), as determined by 4 × 1,500-m incremental testing on-water. Prior to and after each period, the time required to complete the last 1,500-m stage (all-out) of the incremental test (1,500-m time-trial), velocities associated with 2 and 4 mmol·L−1blood lactate (v2[BLa], v4[BLa]) and VO2peakwere determined.

Results:During each period, the mean TID for the entire group was pyramidal (PP1: 84/12/4%, PP2: 80/12/8% and CP: 91/5/4% for Z1, Z2, Z3) and total training time on-water increased from 5.0 ± 0.9 h (PP1) to 6.1 ± 0.9 h (PP2) and 6.5 ± 1.0 h (CP). The individual ranges for Z1, Z2 and Z3 were 61–96, 2–26 and 0–19%. During PP2 VO2peak(25.5 ± 11.4%) markedly increased compared to PP1 and CP and during PP1 v2[bla](3.6 ± 3.4%) showed greater improvement compared to PP2, but not to CP. All variables related to performance improved as the season progressed, but no other effects were observed. With respect to time-trial performance, the time spent in Z1 (r= 0.66,p= 0.01) and total time in all three zones (r= 0.66,p= 0.01) showed positive correlations, while the time spent in Z2 (r= −0.57,p= 0.04) was negatively correlated.

Conclusions:This seasonal analysis of the effects of training revealed extensive inter-individual variability. Overall, TID was pyramidal during the entire period of observation, with a tendency toward improvement in VO2peak, v2[bla], v4[bla]and time-trial performance. During PP2, when the COVID-19 lockdown was in place, the proportion of time spent in Z3 doubled, while that spent in Z1 was lowered; the total time spent training on water increased; these changes may have accentuated the improvement in performance during this period. A further increase in total on-water training time during CP was made possible by reductions in the proportions of time spent in Z2 and Z3, so that more fractions of time was spent in Z1.
A Novel Three-Dimensional Computational Method to Assess Rod Contour Deformation and to Map Bony Fusion in a Lumbopelvic Reconstruction After En-Bloc Sacrectomy
Authors: Eltes Peter Endre , Turbucz Mate , Fayad Jennifer , Bereczki Ferenc , Szőke György , Terebessy Tamás , Lacroix Damien , Varga Peter Pal , Lazary Aron
Published: January 2022

Introduction: En-bloc resection of a primary malignant sacral tumor with wide oncological margins impacts the biomechanics of the spinopelvic complex, deteriorating postoperative function. The closed-loop technique (CLT) for spinopelvic fixation (SPF) uses a single U-shaped rod to restore the spinopelvic biomechanical integrity. The CLT method was designed to provide a non-rigid fixation, however this hypothesis has not been previously tested. Here, we establish a computational method to measure the deformation of the implant and characterize the bony fusion process based on the 6-year follow-up (FU) data.

Materials and Methods: Post-operative CT scans were collected of a male patient who underwent total sacrectomy at the age of 42 due to a chordoma. CLT was used to reconstruct the spinopelvic junction. We defined the 3D geometry of the implant construct. Using rigid registration algorithms, a common coordinate system was created for the CLT to measure and visualize the deformation of the construct during the FU. In order to demonstrate the cyclical loading of the construct, the patient underwent gait analysis at the 6th year FU. First, a region of interest (ROI) was selected at the proximal level of the construct, then the deformation was determined during the follow-up period. In order to investigate the fusion process, a single axial slice-based voxel finite element (FE) mesh was created. The Hounsfield values (HU) were determined, then using an empirical linear equation, bone mineral density (BMD) values were assigned for every mesh element, out of 10 color-coded categories (1st category = 0 g/cm3, 10th category 1.12 g/cm3).

Results: Significant correlation was found between the number of days postoperatively and deformation in the sagittal plane, resulting in a forward bending tendency of the construct. Volume distributions were determined and visualized over time for the different BMD categories and it was found that the total volume of the elements in the highest BMD category in the first postoperative CT was 0.04 cm3, at the 2nd year, FU was 0.98 cm3, and after 6 years, it was 2.30 cm3.

Conclusion: The CLT provides a non-rigid fixation. The quantification of implant deformation and bony fusion may help understate the complex lumbopelvic biomechanics after sacrectomy.
Pancreatic Duct Cells Isolated From Canines Differentiate Into Beta-Like Pancreatic Islet Cells
Authors: Gao Yuhua , Guan Weijun , Bai Chunyu
Published: January 2022

In this study, we isolated and cultured pancreatic ductal cells from canines and revealed the possibility for using them to differentiate into functional pancreatic beta cells in vitro. Passaged pancreatic ductal cells were induced to differentiate into beta-like pancreatic islet cells using a mixture of induced factors. Differentiated pancreatic ductal cells were analyzed based on intracellular insulin granules using transmission electron microscopy, the expression of insulin and glucagon using immunofluorescence, and glucose-stimulated insulin secretion using ELISA. Our data revealed that differentiated pancreatic ductal cells not only expressed insulin and glucagon but also synthesized insulin granules and secreted insulin at different glucose concentrations. Our study might assist in the development of effective cell therapies for the treatment of type 1 diabetes mellitus in dogs.
Antioxidant Effects and Potential Molecular Mechanism of Action of Limonium aureum Extract Based on Systematic Network Pharmacology
Authors: Yang Zhen , Mo Yanan , Cheng Feng , Zhang Hongjuan , Shang Ruofeng , Wang Xuehong , Liang Jianping , Liu Yu , Hao Baocheng
Published: January 2022

Oxidative stress is the redox imbalance state of organisms that involves in a variety of biological processes of diseases. Limonium aureum (L.) Hill. is an excellent wild plant resource in northern China, which has potential application value for treating oxidative stress. However, there are few studies that focused on the antioxidant effect and related mechanism of L. aureum. Thus, the present study combining systematic network pharmacology and molecular biology aimed to investigate the antioxidant effects of L. aureum and explore its underlying anti-oxidation mechanisms. First, the antioxidant activity of L. aureum extracts was confirmed by in vitro and intracellular antioxidant assays. Then, a total of 11 bioactive compounds, 102 predicted targets, and 70 antioxidant-related targets were obtained from open source databases. For elucidating the molecular mechanisms of L. aureum, the PPI network and integrated visualization network based on bioinformatics assays were constructed to preliminarily understand the active compounds and related targets. The subsequent enrichment analysis results showed that L. aureum mainly affect the biological processes involving oxidation-reduction process, response to drug, etc., and the interference with these biological processes might be due to the simultaneous influence on multiple signaling pathways, including the HIF-1 and ERBB signaling pathways. Moreover, the mRNA levels of predicted hub genes were measured by qRT-PCR to verify the regulatory effect of L. aureum on them. Collectively, this finding lays a foundation for further elucidating the anti-oxidative damage mechanism of L. aureum and promotes the development of therapeutic drugs for oxidative stress.
Sinusite fúngica complicada com hipertensão intracraniana e tetraplegia: relato de caso
Authors: Vargas Larissa Jardim , Duarte Bianca Sousa , Pimenta Raissa de Sousa Marinho , Vaz Gleiciane Ramos , Oliveira Andressa Viana , Braga Stephanie Oliveira , de Lucena Talytta Marinho , Manari Adria Luiza Silva , Freire Thayna Caroline Moura , Rabelo Kamylla Paulla Saldanha
Published: January 2022

Objetivo: O objetivo desse relato é descrever um caso raro de sinusite fúngica invasiva crônica que evoluiu com meningite causando síndrome de hipertensão craniana e tetraplegia, demonstrando por meio deste, a importância da realização de uma investigação diagnóstica mais aprofundada mesmo em uma doença considerada de diagnóstico clinico. Assim, evitar o retardo da terapia adequada.
Serum and Salivary Levels of Glucose and Urea in Pregnant and Non-pregnant Women: A Comparative Study
Authors: Alemrajabi Mohammad-Sadegh , Dadkhah Azam , Kasayizadegan-Mahabadi Seyed-Ali , Sadrzadeh-Afshar Maryam-Sadat
Published: January 2022

Background: Pregnancy is one of the most critical periods in the life of most women. Since it is necessary to be aware of the health of the mother and fetus, serum markers (i.e., glucose and urea) need to be monitored during pregnancy. A routine strategy in this area is venipuncture which is applied to measure the level of these markers. It is also a stressful procedure for pregnant women. The purpose of this study was to determine the salivary level of these markers as a stress-free method in pregnant women. Materials and Methods: The samples were collected from 30 pregnant and 30 non-pregnant fasting women. Then, the serum and salivary levels of glucose and urea were measured, analyzed, and compared by photometry. Results: Results indicated that the mean salivary glucose level was 10.2±1.4 mg/dL and 6.4±0.9 mg/ dL in non-pregnant and pregnant women, respectively. In addition, the mean serum glucose level was 106.5±5.3 mg/dL and 82.9±4.5 mg/dL in non-pregnant and pregnant women, respectively. Further, the mean salivary urea level was 37.1±3.3 mg/dL in non-pregnant women and 27.1±1.9 mg/dL in pregnant women. Moreover, the mean serum urea level was 26.9±1.9 mg/dL and 19.5±2.3 mg/dL in non-pregnant and pregnant women, respectively. Conclusion: Serum and salivary levels of glucose and urea in pregnant women were lower than those in non-pregnant women, and there was a positive correlation between serum and salivary levels. Therefore, it seems that saliva can be a substitute for serum regarding the measurement of glucose and urea levels.
BRAND MANAGEMENT PRO-SOCIAL ACTIVITIES AS AN EFFECTIVE ELEMENT OF IMAGE STRATEGY
Authors: Nocoń Marek , Różycka Magdalena
Published: January 2022

The aim of this article is to research the effectiveness of pro-social actions in the building of a brand image and if it really might be an effective determinant of marketing actions – which has an influence on consumer choices: chosen aspects of pro-social actions used in the strategies of NIVEA and Rossmann drugstores are explored here. In addition, analyzes of pro-social actions during pandemic was carried out. It is proved that companies which took a part in relief efforts during the crisis were appreciated by consumers; which helped when it came to creating brand value. The most important question seems to be not “Whether to invest in CSR?” but “How to invest?” This issue is sure to become the subject of numerous research projects and analyzes in the future.
BELAJAR BERSAMA ALAM SEBAGAI BENTUK PENERAPAN EKOLITERASI PADA SEKOLAH ALAM
Authors: Rachmawati Annisa , Minsih
Published: January 2022

Tujuan dilakukan penelitian ini adalah: 1) Menjelaskan tahapan pembelajaran di SD Muhammadiyah Alam Surya Mentari Surakarta. 2) Menjelaskan tahapan Belajar Bersama Alam sebagai bentuk penerapan ekoliterasi di SD Muhammadiyah Alam Surya Mentari Surakarta. 3) Menjelaskan peran guru dalam pelaksanaan Belajar Bersama Alam sebagai bentuk penerapan ekoliterasi di SD Muhammadiyah Alam Surya Mentari Surakarta. 4) Menjelaskan kendala pelaksanaan Belajar Bersama Alam sebagai bentuk penerapan ekoliterasi di SD Muhammadiyah Alam Surya Mentari Surakarta. Metode di dalam penelitian ini menggunakan metode kualitatif. Dan teknik pengumpulan data menggunakan metode wawancara dan dokumentasi. Berdasarkan dari peneliatian yang sudah dilakukan diperoleh hasil: a) Tahapan proses pembelajaran yang dilakukan di SD Muhammadiyah Alam Surya Mentari Surakarta dimulai dari Tahap Perencanaan, Tahap Pelaksanaan, Tahap Evaluasi, dan Tahap Tindak Lanjut. b) Tahapan Belajar diawali dari tahap perencanaan. Kedua, tahap pelaksanaan yaitu peserta didik langsung terjun ke alam secara langsung. Ketiga, tahap evaluasi, dan keempat yaitu tahap tindak lanjut. c) Peran guru dalam pelaksanaan Belajar Bersama Alam salah satunya sebagai rolle model , yaitu guru memberikan suri tauladan dan akhlaq yang baik d) Kendala dalam pelaksanaan Belajar Bersama Alam sebagai bentuk penerapan ekoliterasi di sekolah alam yaitu salah satunya faktor cuaca dan minimnya laboratorium alam di lingkup sekolah.
PENGARUH MODEL PEMBELAJARAN BERBASIS MASALAH TIPE MEANS ENDS ANALYSIS (MEA) TERHADAP KEMAMPUAN BERPIKIR KRITIS MATEMATIS
Authors: Hevitria  , Yuanita  , Arsisari Ayen
Published: January 2022

Dalam hal ini peneliti meneliti tentang pengaruh model pembelajaran berbasis masalah tipe means ends analysis (MEA) terhadap kemampuan berpikir kritis matematis siswa. Tujuan penelitian ini untuk mengetahui apakah terdapat pengaruh model pembelajaran berbasis masalah tipe Means ends analysis (MEA) terhadap kemampuan berpikir kritis matematis. Penelitian ini menggunakan pendekatan kuantitatif dengan metode quasi experimental desain. Adapun desainnya menggunakan Nonequivalent Control Group Design. Penelitian ini dilakukan di SD Negeri 6 Pangkalpinang dengan menerapkan model pembelajaran berbasis masalah tipe Means ends analysis (MEA) dan  model konvensional. Populasi dalam penelitian ini adalah seluruh siswa kelas V SD Negeri 6 Pangkalpinang yang berjumlah dua kelas, sampel dipilih dengan teknik pengambilan purposive sampling. Subjek penelitian ini adalah kelas V SD yang terdiri dari kelas VA berjumlah 32 orang dan kelas V B berjumlah 32 orang. Teknik pengumpulan data menggunakan tes kemampuan berpikir kritis berbentuk uraian. Berdasarkan hasil perhitungan dengan uji pooled the variance diperoleh bahwa  = 7,865 >  = 1,694. Maka dapat disimpulkan H1 diterima, artinya terdapat pengaruh model pembelajaran berbasis masalah tipe Means ends analysis (MEA) terhadap kemampuan berpikir kritis matematis di kelas V SD Negeri 6 Pangkalpinang.
Latrine site selection of raccoon dogs in a hilly area in north-eastern Japan
Authors: Watanabe Kazuma , Kumagai Nami , Saito Masayuki U.
Published: January 2022

We evaluated the environment types of raccoon dog latrine sites in the hilly areas of north-eastern Japan. We conducted a route census in the spring and autumn of 2020 to record the latrine sites and analysed the relationship between the presence or absence of latrine sites and environmental factors, namely, topographic position index (TPI), slope, normalised difference vegetation index (NDVI), and vegetation type for each season. To investigate the space use of raccoon dogs, we also conducted camera trapping from July to November 2020 along the spring survey route. We analysed the relationship between the occurrence frequency of raccoon dogs and TPI, slope angle, NDVI, and vegetation type. The analysis showed that latrine sites tended to be located at sites with a high TPI (topography closer to the ridge) in both seasons. However, the occurrence of latrine sites in broadleaf forests was significantly higher in autumn. The frequency of raccoon dogs, based on camera-trap footage, was significantly higher at sites with gentle slopes; although the environment and space used by raccoon dogs at these sites differed. Raccoon dogs possibly select visually and olfactorily conspicuous sites on the ridge as latrine sites to facilitate odour dispersal. In addition, broadleaf forests in autumn are considered important feeding grounds for raccoon dogs, suggesting that the latrine sites were formed near foraging sites.