bluuebunny/crossref_metadata_2025 huggingface dataset:
Penderita Kusta Baru di RSUD DR. H. Chasan Boesoirie Ternate
Authors: Sangadji Muhammad Radhy , Hartati Hartati , Hidayat Ferdian , Toka Wahyunita Do
Published: February 2024

ABSTRACT Leprosy is a skin disease caused by Mycobacterium leprae. Indonesia is the 3rd largest country in the world with a high incidence of leprosy, after Brazil and India. This study aims to determine the profile of new leprosy patients at Dr. H. Chasan Boesoirie Ternate for the period January 2019 – December 2022. The method used in this research is a retrospective descriptive method, with a cross sectional approach. The results showed that the most age group were new leprosy patients, adult patients with 28 patients (66.7%), the most gender was male 24 patients (57.1%), according to the results of the bacterial index examination was 1 + with 25 patients (59.5%), type of leprosy was the multibacillary type with 41 patients (97.6%), and based on the most contact history there was no contact history with 37 patients (88.1%). It can be concluded that the most new leprosy patients found were the adult age group, the most sex was male, according to the results of the bacterial index examination the most was 1+, the most type of leprosy was the multibacillary type, and based on the contact history group the most was no contact history.  Keywords: New Leprosy, Mycobacterium Leprae, Ternate  ABSTRAK Penyakit kusta juga dikenal dengan penyakit lepra merupakn penyakit kulit yang disebabkan oleh Mycobacterium leprae. Indonesia merupakan negara urutan ke-3 terbanyak di dunia dengan insidensi kusta yang tinggi, sesudah Brazil dan India.. Penelitian ini bertujuan untuk Untuk mengetahui profil penderita kusta baru di RSUD Dr. H. Chasan Boesoirie Ternate periode Januari 2019 – Desember 2022. Metode yang digunakan pada penelitian ini adalah metode deskriptif retrospektif, dengan pendekatan cross sectional. Hasil penelitian menunjukkan Pasien kusta baru dengan kelompok umur terbanyak yaitu pasien dewasa dengan jumlah 28 pasien (66,7 %), jenis kelamin paling banyak laki laki dengan jumlah 24 pasien (57,1 %), distribusi menurut hasil pemeriksaan bacterial index terbanyak adalah 1+ dengan jumlah 25 pasien (59,5 %), berdasarkan tipe kusta terbanyak adalah tipe mulitibasiler dengan jumlah 41 pasien (97,6 %), dan berdasarkan riwayat kontak terbanyak adalah tidak terdapat riwayat kontak dengan jumlah 37 pasien (88,1 %). Dapat disimpulkan bahwa pasien kusta baru yang paling banyak ditemukan adalah kelompok umur dewasa, jenis kelamin terbanyak adalah laki laki, menurut hasil pemeriksaan bacterial index terbanyak adalah 1+, tipe kusta terbanyak adalah tipe multibasiler, dan berdasarkan kelompok riwayat kontak terbanyak adalah tidak terdapat riwayat kontak. Kata Kunci: Kusta Baru, Mycobacterium Leprae, Ternate
Kemampuan Penurunan Kadar Kolesterol oleh Ekstrak Metanol Cinnamomum Burmanii Secara In Vitro
Authors: Zulfa Lathifah Dzakiyyah , Salim Dessyani , Natasha Margaretha Maria Odilia , Sumilat Lawrent Ernts , Djaya Kevin Gilbert
Published: February 2024

ABSTRACT Cholesterol is normally needed by human body as cell fluidity regulator, a precursor for the formation of steroid hormones, and precursor for the formation of bile acids, but excess cholesterol levels in the blood will increase the risk of cardiovascular disease. Some cholesterol lowering agents have side effects so exploration of herbal plants that can lower cholesterol levels is necessary. This study aims to prove the cholesterol binding ability of Cinnamomum burmanii methanol extract. In Vitro, through photometric measurements, cholesterol binding was carried out by the methanol extract of Cinnamomum burmanii. The absorbance value at the 526nm wave is then converted into a percentage of the reduction in cholesterol levels. The percentage of the reduction in cholesterol levels increased as the concentration of Cinnamomum burmanii methanol extract increased. The increasing percentage of reduction in cholesterol levels is based on the bonds formed from the contents of Cinnamomum burmanii, such as tannin and saponin. Keywords: Cholesterol, Cinnamomum Burmanii, ldl  ABSTRAK Kolesterol secara normal dibutuhkan tubuh sebagai pengatur fluiditas sel, prekursor pembentukan hormon steroid, dan prekursor pembentukan asam empedu, namun kelebihan kadar kolesterol dalam darah akan meningkatkan risiko penyakit kardiovaskular. Beberapa agen penurun kadar kolesterol darah memiliki efek samping sehingga eksplorasi tanaman herbal yang mampu menurunkan kadar kolesterol diperlukan. Penelitian ini bertujuan untuk membuktikan kemampuan pengikatan kolesterol oleh ekstrak metanol kayu manis. Secara In Vitro melalui pengukuran fotometrik, dilakukan pengikatan kolesterol oleh ekstrak metanol Cinnamomum burmanii. Nilai absorbansi pada gelombang 526nm selanjutnya dikonversi menjadi persentase penurunan kadar kolesterol. Persentase penurunan kadar kolesterol bertambah tinggi seiring dengan bertambahnya konsentrasi ekstrak metanol kayu manis. Meningkatnya persetase penurunan kadar kolesterol didasari adanya ikatan yang terbentuk dari kandungan kayu manis, seperti tannin dan saponin. Kata Kunci: Ldl, Kayu Manis, Kolesterol
Intervensi Pencegahan Luka Tekan pada Pasien Imobilisasi: Rapid Review
Authors: Kosim Kosim , Nurhakim Furkon
Published: February 2024

ABSTRACT A pressure ulcer is skin and tissue damage that occurs due to pressure on the surface so that it interferes with the blood supply to the area. Patients can experience pressure ulcers due to hospitalization (hospital-acquired pressure injury) where there is damage to skin integrity during hospitalization, so health workers need to prevent pressure ulcers in patients. to identify interventions to prevent pressure ulcers in immobilized patients. rapid review by searching for articles in three databases; namely CINAHL, PubMed, and Google Scholar. Inclusion criteria in this literature were Adults (18-44 years), published in the last 10 years, English or Indonesian articles, full text, randomized controlled trial, quasi-experimental, cross-sectional, and cohort studies. we found four interventions that could be done to prevent pressure ulcers in immobilized patients; namely protective equipment, massage, topical, and dressings. four interventions have their respective strengths and weaknesses. Researchers recommend a topical olive oil intervention that can be applied in Indonesia with the justification that in addition to preventing the incidence of pressure ulcers, giving olive oil also costs less and is considered affordable. Keywords: Intervention, Immobilized Patient, Pressure Ulcer  ABSTRAK luka tekan merupakan kerusakan kulit dan jaringan yang terjadi akibat tekanan di permukaan sehingga mengganggu suplai darah ke area tersebut. Pasien dapat mengalami luka tekan akibat hospitalisasi (hospital-acquired pressure injury) di mana terjadi kerusakan integritas kulit selama pasien dirawat inap sehingga penting bagi tenaga kesehatan untuk melakukan pencegahan luka tekan pada pasien.  mengidentifikasi intervensi yang dapat dilakukan untuk mencegah luka tekan pada pasien imobilisasi.  Tinjauan cepat dengan pencarian artikel di tiga basis data CINAHL, PubMed dan Google Scholar. Kriteria inklusi dalam literature ini yaitu Dewasa (18-44 tahun), diterbitkan dalam 10 tahun terakhir, artikel Bahasa Inggris atau Bahasa Indonesia, artikel dengan naskah lengkap , jenis penelitian uji acak terkendali, kuasi eksperimen, potong lintang dan studi kohort ditemukan empat intervensi yang dapat dilakukan untuk mencegah terjadinya luka tekan pada pasien imobilisasi yaitu alat pelindung, masase, topikal dan balutan. keempat intervensi memiliki kekuatan dan kelemahannya masing-masing. Peneliti merekomendasikan intervensi topikal minyak zaitun yang dapat diterapkan di Indonesia dengan penguatan bahwa selain dapat mencegah kejadian luka tekan, pemberian minyak zaitun juga menghabiskan biaya yang lebih murah dan dinilai terjangkau. Kata Kunci: Intervensi, Luka Tekan, Pasien Imobilisasi
Hubungan Tingkat Stres dengan Kejadian Insomnia pada Mahasiswa Keperawatan Tingkat Awal
Authors: Gasril Pratiwi , Devita Yeni , Fadli Nanang
Published: February 2024

ABSTRACT Stress is the inability to cope with the threats faced by mental, physical, emotional, and spiritual human beings, which at some point can affect the physical health of the human being. Insomnia is the inability to meet sleep needs, both in quality and quantity. The purpose of this study was to determine the relationship between stress levels and the incidence of insomnia in nursing students. The Research Method uses a correlative descriptive research design using a cross sectional approach. The sample in this study amounted to 50 respondents, with a Stratified Sampling technique. The instruments used in this study were the Kessler Psychological Distress Scale and Insomnia Rating Scale questionnaires disseminated with Goggle Form links. The results of the study found that as many as 10% of students were not stress, 40% were mild stress, 32% were moderate stress, and 18% were severely stressed. As many as 4% of students had no insomnia, 54% mild insomnia, 20% moderate insomnia, and 22% severe insomnia. From the results of data analysis using the help of the SPSS 16 computer program whose meaning level ρ ≤ α (0.001 ≤ 0.005), it was concluded that there is a relationship between the level of stress and the level of insomnia incidence in nursing students. Keywords: Stress, Insomnia, Students, Nursing  ABSTRAK Stres adalah ketidakmampuan mengatasi ancaman yang dihadapi oleh mental, fisik, emosional, dan spiritual manusia, yang pada suatu saat dapat mempengaruhi kesehatan fisik manusia tersebut. Insomnia adalah ketidakmampuan memenuhi kebutuhan tidur, baik secara kualitas maupun kuantitas. Tujuan penelitian ini adalah diketahuinya hubungan tingkat stress dengan kejadian insomnia pada mahasiswa keperawatan tingkat awal. Metode Penelitian menggunakan desain penelitian deskriptif  korelatif dengan menggunakan pendekatan cross sectional. Sampel dalam penelitian ini berjumlah 50 responden, dengan teknik pengambilan sampel Stratified Sampling. Instrumen  yang digunakan dalam penelitian ini adalah kuesioner Kessler Psychological Distress Scale  dan Insomnia Rating Scale  yang disebarkan dengan link Goggle Form. Hasil Penelitian didapatkan hasil  sebanyak 10% mahasiswa tidak stress, 40% stress ringan, 32% stress sedang, dan 18% stress berat. Sebanyak 4% mahasiswa tidak insomnia, 54% insomnia ringan, 20% insomnia sedang, dan 22% insomnia berat. Dari hasil Analisa data dengan menggunakan bantuan program computer SPSS 16 yang tingkat pemaknaan ρ ≤ α (0,001 ≤ 0,005), Disimpulkan bahwa ada hubungan antara tingkat stress dengan kejadian insomnia pada mahasiswa Keperawatan tingkat awal. Kata Kunci: Stres, Insomnia, Mahasiswa, Keperawatan
Hubungan Pengetahuan Perawat tentang Pemberian Obat dengan Penerapan Prinsip 6 (Enam) Benar Pada Pasien di Rumah Sakit Rumkitban 02.09.04 Kerinci
Authors: Suzana Moza , Herlina Defvi
Published: February 2024

ABSTRACT Patient safety is the main reference and principle in the health servive process in a health provider institution. The world helath organization (WHO) in 2020 reported that in several countries there were cases of as many as 70% of medication erros that caused permanent disability in patients. Medication erros also often occur in the caused permanent disability in hospitals. Errors in drug administration in Indonesia were ranked first (24.9%) of the top 10 reported incident. The aim of this research is to determine the relationship between nurses' knowledge about administering medication and the 6 principles of correct medication. The purpose of this study was to all nurses who worked at the Rumkitban Hospital amounting to 32 respondents. The samples taken were 32 respondents who were obtained using a total sampling technique with a cross sectional approach. The ingtrument used is a questionnaire. The data in this study were processed using the chi-square test with a signifixanxe level of P= 0,005. From the study, it was found that there was a significant relationship between nurses knowledge about drug administration and application of the 6 correct principles of medicine (P=0,017). The recommended advice for nurses and hospitals is to seek as much information as possible about the application of the 6- drug principle so that patient safety is guaranted Keywords: Nurses, Knowledge and Application of the Six Right Medicine Principles  ABSTRAK Keselamatan pasien merupakan acuan dan prinsip utama dalam proses pelayanan kesehatan di sebuah lembaga penyedia kesehatan. World Health Organitation (WHO) pada tahun 2020 melaporkan bahwa beberapa negara  terjadi   kasus   sebanyak 70%  kesalahan pengobatan hingga menyebabkan kecacatan permanen pada pasien. Kesalahan pengobatan juga sering kali terjadi dalam proses pengobatan, khususnya di Rumah Sakit. Kesalahan dalam pemberian obat di Indonesia menduduki peringkat pertama (24,9 %) dari 10 besar insiden yang dilaporkan. Tujuan penelitian ini adalah untuk mengetahui hubungan pengetahuan perawat tentang pemberian obat dengan prinsip 6 benar obat. Populasi dalam penelitian ini adalah seluruh perawat yang bekerja di rumah sakit rumkitban yang berjumlah 32 responden. Sampel yang diambil berjumlah 32 responden yang di peroleh menggunakan teknik total sampling dengan pendekatan Cross Sectional. Instrumen yang digunakan adalah kuesioner. Data dalam penelitian ini diolah dengan menggunakan uji chi-square dengan tingkat kemaknaan P = 0,005. Dari hasil penelitian ini diperoleh bahwa ada hubungan yang signifikan antara pengetahuan perawat tentang pemberian obat dengan penerapan prinsip 6 benar obat (P = 0,017). Saran yang dianjurkan yaitu bagi perawat dan rumah sakit adalah mencari infoemasi sebanyak-banyaknya tentang penerapan prinsip 6 obat agar keselamatan pasien terjamin. Kata Kunci: Perawat, Pengetahuan dan Penerapan Prinsip Enam Benar Obat
Analisis Pemahaman Pasien Terhadap Hak dan Kewajiban Pasien di Klinik Pratama Kelambir
Authors: Sulisna Aida , Ilham Muhammad Adiul , Pohan Hana Dhini Julia
Published: February 2024

ABSTRACT The rights and obligations of patients are regulated in Law number 36 of 2009 concerning health. Various patient rights include; the right to information, the right to give consent, the right to medical secrets and the right to a second opinion. Meanwhile, the patient's obligation is to provide correct information to the doctor, comply with the doctor's or nurse's recommendations, provide appropriate compensation for services and the patient also has an obligation not to force his wishes to be carried out by the doctor if it is contrary to the freedom and nobility of the doctor's profession. To analyze patient understanding of patient rights and obligations at the Kelambir Pratama Clinic. The method used is quantitative with a cross sectional analytical survey approach. The research population is 82 patients in 2023 and the sample is Total Sampling. Sampling was carried out using the Accidental Sampling method. Show that there is an influence of education with a p-value= 0.000<0.05, an environmental influence with a p-value= 0.000<0.05 and an influence of information sources with a p-value= 0.000<0.05 on the patient's level of understanding. The conclusion is that there is an influence of education, environment and information sources on the level of understanding of patient rights and obligations at the Pratama Klambir Clinic.  Keywords: Understanding Patient Rights and Obligations  ABSTRAK Hak dan kewajiban pasien diatur dalam Undang-Undang nomor 36 tahun 2009 tentang kesehatan. Macam- macam hak pasien meliputi; hak atas infomasi, hak untuk memberikan persetujuan, hak atas rahasia kedokteran dan hak atas pendapat kedua. Sedangkan kewajiban pasien adalah memberikan informasi yang benar kepada dokter, mematuhi anjuran dokter atau perawat, memberi imbalan jasa yang layak dan pasien juga mempunyai kewajiban untuk tidak memaksakan keinginannya agar dilaksanakan oleh dokter apabila berlawanan dengan kebebasan dan keluhuran profesi dokter. Untuk menganalisis Pemahaman Pasien Terhadap Hak dan Kewajiban Pasien Di Klinik Pratama Kelambir. Metode yang digunakan adalah kuantitatif dengan pendekatan survey analitic cross sectional. Populasi penelitian adalah pasien pada tahun 2023 sebanyak 82 pasien dan sampel adalah Total Sampling. Penarikan sampel dilakukan dengan metode Accidental Sampling. Menunjukkan bahwa ada pengaruh pendidikan dengan p-value= 0,000<0,05, pengaruh lingkungan dengan p-value= 0,000<0,05 dan pengaruh sumber informasi dengan p-value= 0,000<0,05 terhadap tingkat pemahaman pasien. Kesimpulan ada pengaruh pendidikan, lingkungan dan sumber informasi terhadap tingkat pemahaman hak dan kewajiban pasien di Klinik Pratama Klambir Kata Kunci: Pemahaman Hak dan Kewajiban Pasien
Hubungan Antara Kejenuhan Kerja (Burnout) dengan Motivasi Kerja Pada Perawat Pelaksana
Authors: Diyanto Velani Prastika , Supratman Supratman
Published: February 2024

ABSTRACT Nurses are an important component in realizing quality health services, apart from that, nurses have medical services that have been recognized by the government. Nurses are in all units in the hospital, one of which is in the inpatient unit. The aim of this research is to determine the relationship between burnout and work motivation in executive nurses. Utilizing probability sampling techniques, the approach is quantitative. A total of 110 people participated in the study. The Maslach Burnout Inventory-Human Service Survey (MBI-HSS) questionnaire, which consists of 22 questions in the form of a Likert model scale with response options ranging from 1 to 4, is the tool utilized in this study. 1 denotes never, 2 seldom, 3 frequently, and 4 constantly. The study's findings indicate a strong correlation between implementation nurses work motivation and burnout. It can be concluded that the majority of nurse respondents at RSUD Dr. Moewardi is in the 30-40 year age range, which is considered a productive period with a high level of productivity.  Keywords: Burnout, Executive Nurses, Work Motivation  ABSTRAK Selain berperan penting dalam penyediaan layanan kesehatan berkualitas tinggi, perawat juga memiliki spesialisasi medis yang diakui secara resmi. Setiap unit rumah sakit, termasuk bangsal rawat inap, memiliki staf perawat. Tujuan penelitian ini adalah mengetahui Hubungan Antara Kejenuhan Kerja (Burnout) Dengan Motivasi Kerja Pada Perawat Pelaksana. Metode yang digunakan adalah kuantitatif dengan teknik probability sampling. Responden yang diteliti berjumlah 110 responden. Penelitian ini menggunakan kuesioner Maslach Burnout Inventory-Human Service Survey (MBI-HSS) yang terdiri dari 22 pertanyaan dengan skala model Likert dengan pilihan jawaban berkisar antara 1 sampai 4. 1 menunjukkan tidak pernah, 2 jarang, 3 sering, dan 4 terus-menerus. Temuan penelitian menunjukkan bahwa kelelahan dan motivasi penerapan perawat dalam pekerjaannya berkorelasi secara signifikan. Dapat disimpulkan bahwa sebagian besar responden perawat di RSUD Dr. Moewardi berada pada rentang usia 30-40 tahun, yang dianggap masa produktif dengan tingkat produktivitas yang tinggi. Kata kunci: Kejenuhan Kerja, Motivasi Kerja, Perawat Pelaksana
Efektivitas Media Lembar Balik Terhadap Tingkat Pengetahuan dan Sikap Ibu dalam Pemberian MP-ASI
Authors: Hasanah Devi Miftahul , Lesmana Hendy
Published: February 2024

ABSTRACT Toddlers in the developing phase of growth and development are more susceptible to health issues arising from dietary deficiencies. Optimal nutrition during the early stages of life significantly influences the child's physical, cognitive, and social development. Parental variables associated with nutritional issues in toddlers encompass parental education and occupation and mother knowledge, attitudes, and behavior about nutrition. To enhance maternal knowledge, it is necessary to develop health education programs that promote awareness and understanding of health requirements. This will facilitate the adoption of healthy behaviors. This study aims to assess the efficacy of flip-sheet media in enhancing the knowledge and attitudes of mothers in delivering weaning food. The research approach employed in this study is quantitative, utilizing a Quasi-Experimental design with a post-test-only Control Group Design. The sample size for this study consisted of 64 moms with children between the ages of 6 and 24 months. The research consisted of a total sample size of 55 respondents, selected using the Purposive Sampling approach. The Mann-Whitney U test was employed to evaluate the data. This study demonstrates a notable correlation between the knowledge and attitude of mothers in providing weaning food through a flip sheet medium. Future researchers should conduct comparative studies between one health promotion medium and other media. Keywords: Flip-sheets,  Weaning Food, Health Education, Mother’s Knowledge and Attitudes  ABSTRAK Balita berada pada masa awal pertumbuhan dan perkembangan, maka mereka sangat rentan mengalami gangguan kesehatan akibat masalah gizi. Awal gizi yang baik akan sangat berakibat pada kehidupan yaitu pada perkembangan fisik, kognitif dan sosial anak. Faktor orang tua yang berhubungan dengan masalah gizi pada balita, antara lain pendidikan dan pekerjaan orang tua, serta pengetahuan, sikap, dan perilaku ibu tentang gizi. Upaya untuk meningkatkan pengetahuan ibu perlu dilaksanakan penyuluhan kesehatan untuk meningkatkan pengetahuan dan sikap tentang kebutuhan kesehatan agar mudah terjadinya perilaku sehat. Tujuan penelitian ini adalah menganalisis efektivitas media lembar balik terhadap tingkat pengetahuan dan sikap ibu dalam pemberian MP-ASI. Metode penelitian ini adalah kuantitatif dengan desain Quasi Experimental dengan Post Test Only Control Group Design. Populasi dalam penelitian ini sebanyak 64 ibu yang memiliki balita usia 6-24 bulan. Jumlah sampel penelitian ini adalah 55 responden yang dipilih menggunakan teknik Purposive Sampling. Data dianalisis dengan uji Mann Whitney U. Berdasarkan hasil penelitian ini menunjukkan ada pengaruh yang signifikan antara pengetahuan dan sikap ibu dalam pemberian MP-ASI terhadap media lembar balik. Peneliti selanjutnya direkomendasikan untuk membandingkan salah satu media promosi kesehatan dengan media lainnya. Kata Kunci: Lembar Balik, MP-ASI, Pendidikan Kesehatan, Pengetahuan dan Sikap Ibu
Pengetahuan Remaja Putri tentang Senam Dismenorea Wilayah RT 002 RW 014 Kelurahan Jatibening Tahun 2023
Authors: Anisa Delya , Rahmadyanti Rahmadyanti
Published: February 2024

ABSTRACT The lack of knowledge among adolescent girls about Dismenorea exercises is a problem that needs attention. Dismenorea, if not properly managed, can disrupt activities and affect mental and physical functions such as weakness, restlessness, depression, severe cramps, and accumulation of menstrual blood in the pelvic cavity. Long-term use of pharmacological treatments can also lead to side effects such as kidney damage and bone thinning. This study aims to evaluate the knowledge of adolescent girls about Dismenorea exercises. The study design is descriptive, with a population consisting of all adolescent girls in Rt 002 Rw 014 of Jatibening Village. The research sample consists of 100 respondents, selected randomly. Data collection was done through a questionnaire. The results of the study show that out of 100 respondents in Rt 002 Rw 014 of Jatibening Village, 34 (34%) respondents have insufficient knowledge, 27 (27%) respondents have sufficient knowledge, and 39 (39%) respondents have good knowledge. Based on the research findings, it can be concluded that the knowledge of adolescent girls in Rt 002 Rw 014 is still lacking. Therefore, further research can explore the factors that contribute to the lack of knowledge among adolescent girls about Dismenorea exercises. Keywords: Knowledge, Adolescent Girls, Dismenorea Exercises  ABSTRAK Kurangnya pengetahuan remaja putri tentang senam Dismenorea adalah masalah yang perlu diperhatikan. Dismenore, jika tidak ditangani dengan baik, dapat mengganggu aktivitas dan mempengaruhi fungsi mental dan fisik seperti kelemahan, kegelisahan, depresi, kram yang parah, dan penumpukan darah menstruasi di rongga panggul. Penggunaan pengobatan farmakologis dalam jangka waktu yang lama juga dapat menyebabkan efek samping seperti kerusakan ginjal dan penipisan tulang. Penelitian ini bertujuan untuk mengevaluasi pengetahuan remaja putri tentang senam Dismenorea.  Desain penelitian ini bersifat deskriptif, dengan populasi yang terdiri dari semua remaja putri di Wilayah Rt 002 Rw 014 Kelurahan Jatibening. Sampel penelitian terdiri dari 100 responden, yang dipilih secara acak. Pengumpulan data dilakukan melalui kuesioner. Hasil penelitian menunjukkan bahwa dari 100 responden di Wilayah Rt 002 Rw 014 Kelurahan Jatibening, sebanyak 34 (34%) responden memiliki pengetahuan yang kurang, 27 (27%) responden memiliki pengetahuan yang cukup, dan 39 (39%) responden memiliki pengetahuan yang baik. hasil penelitian, dapat disimpulkan bahwa pengetahuan remaja putri di wilayah Rt 002 Rw 014 masih kurang. Oleh karena itu, penelitian selanjutnya dapat mengeksplorasi faktor-faktor apa saja yang menyebabkan kurangnya pengetahuan remaja putri tentang senam Dismenorea. Kata Kunci: Pengetahuan, Remaja Putri, Senam Dismenore
Efektivitas Terapi Musik K-Pop Terhadap Penurunan Tingkat Kecemasan Sebelum Pertunjukan Pada Dancer Remaja di Yapemri Depok
Authors: Fajariyah Nur , Utami Ladies Tri
Published: February 2024

ABSTRACT Music therapy has the effect of sedation, stimulates the limbic system, which is the system that manages emotions. Anxiety in adolescents arises due to many reasons, such as the fear of failing in doing something, for example when you want to do a dance performance, which can cause excessive worry about failing, things like that can interfere with concentration and will also cause anxiety. Dancers who experience anxiety can affect their appearance before the show, and anxiety can be controlled by relaxing with therapy, one of which is music therapy. To determine the relationship between the effectiveness of K-POP music therapy and the decrease in the anxiety level of young dancers at YAPEMRI Depot. The methodology used in this study is a Quasi-Experimental method, by carrying out complementary therapy, namely music therapy, which is carried out for 15 minutes, and is also carried out regularly for 6 days. From the results of the study it was found that there was effectiveness of K-POP music therapy on reducing anxiety levels before competing in young dancers at YAPEMRI Depot with a value of p=0.000 or p<0.05. This study proved the effectiveness of K-POP music therapy in reducing anxiety levels. Keywords: Music Therapy, Anxiety, Adolescents, Dancer.  ABSTRAK Terapi musik memiliki efek yaitu sedasi, merangsang sistem limbik yang dimana sistem ini merupakan yang mengelola emosi. Kecemasan pada remaja timbul akibat banyaknya sebab, seperti takutnya mengalami kegagalan dalam melakukan suatu hal, contohnya saat ingin melakukan pertunjukan tari, yang dapat menimbulkan rasa khawatir yang berlebihan akan mengalami kegagalan, hal seperti itu dapat menggangu konsenterasi dan juga akan menyebakan terjadinya kecemasan. Dancer yang mengalami kecemasan dapat mempengaruhi penampilan sebelum pertunjukan, dan kecemasan dapat dikontrol dengan melakukan relaksasi menggunakan terapi, salah satunya yaitu terapi musik. Mengetahui hubungan efektivitas terapi musik K-POP terhadap penurunan tingkat kecemasan dancer remaja di YAPEMRI Depok. Metodelogi yang digunakan pada penelitian ini merupakan metode Quasi Eksperimental, dengan melakukan terapi komplementer yaitu terapi musik, yang dilakukan selama 15 menit, dan juga dilakukan secara rutin selama 6 hari.  Dari hasil penelitian didapatkan bahwa adanya efektivitas terapi musik K-POP terhadap penurunan tingkat kecemasan sebelum bertanding pada dancer remaja di YAPEMRI Depok dengan nilai p=0,000 atau p<0,05. Dalam penelitian ini terbukti adanya efektivitas terapi musik K-POP terhadap penurunan tingkat kecemasan Kata Kunci: Terapi Musik, Kecemasan, Remaja, Penari.
Hubungan Pengetahuan dan Sikap Dengan Pelaksanaan Perawatan Metode Kanguru
Authors: Hanum Parida , Winasis Tyas , Sartika Titin , Tambunan Tia Oktaviani , Hulu Trinias Oktaviani , Siregar Try Hidayati
Published: February 2024

ABSTRACT The kangaroo method of care is a method that has been proven to reduce the incidence of infections, breastfeeding problems and increase maternal satisfaction and improve the relationship between mother and baby. WHO reports that LBW has a risk of death 20 times greater than babies with normal birth weight. LBW are susceptible to infectious diseases because the body's anti-immune substances are less than perfect. The aim of this research was to determine the relationship between knowledge and attitudes with the implementation of the kangaroo method of care at UPT Puskesmas Sedinginan. This type of research uses descriptive correlation to see a relationship between knowledge and attitudes and the implementation of kangaroo method care. The population in this study were all mothers who gave birth to LBW babies, namely 50 people. The sample in this study was all mothers who gave birth to LBW babies, namely 50 people. Sampling in this study used the Total Sampling technique. Analysis of research data is univariate and bivariate analysis. The results of the research explain that there is a relationship between mother's knowledge and attitudes and the implementation of kangaroo method care with a P value of 0.006. It is hoped that this research suggestion can provide knowledge information to mothers about kangaroo care methods. Keywords: Knowledge, Attitude, Kangaroo Method  ABSTRAK Perawatan metode kanguru merupakan salah satu metode yang terbukti dapat menurunkan kejadian infeksi, masalah menyusui dan meningkatkan kepuasan ibu serta meningkatkan hubungan antara ibu dan bayi. WHO melaporkan, BBLR memiliki resiko kematian 20 kali lebih besar dari bayi dengan berat badan lahir normal. BBLR mudah mengalami penyakit infeksi karena zat anti kekebalan tubuh kurang sempurna. Tujuan penelitian ini adalah untuk mengetahui hubungan pengetahuan dan sikap dengan pelaksanaan perawatan metode kanguru di UPT Puskesmas Sedinginan. Jenis penelitian ini menggunaan deskriptif korelasi untuk melihat sebuah hubungan antara pengetahuan dan sikap dengan pelaksanaan perawatan metode kanguru. Populasi dalam penelitian ini adalah  seluruh ibu yang melahirkan bayi BBLR yaitu sebanyak 50 orang. Sampel dalam penelitian ini adalah seluruh ibu yang melahirkan bayi BBLR yaitu sebanyak 50 orang. Pengambilan sampel pada penelitian ini menggunakan teknik Total Sampling. Analisa data penelitian adalah anlias univariat dan bivariat. Hasil penelitian menjelaskan bahwa ada hubungan antara pengetahuan dan sikap ibu dengan pelaksanaan perawatan metode kanguru di UPT Puskesmas Sedinginan dengan nilai Pvalue 0,006. Saran penelitian ini diharapkan dapat memberikan informasi pengetahuan kepada ibu tentang perawatan metode kanguru. Kata Kunci: Pengetahuan, Sikap, Metode Kanguru
CD8+ NKs as a potential biomarker of complete response and survival with lenalidomide plus R-GDP in the R2-GDP-GOTEL trial in recurrent/refractory diffuse large B cell lymphoma
Authors: Hontecillas-Prieto Lourdes , García-Domínguez Daniel J. , Palazón-Carrión Natalia , Martín García-Sancho Alejandro , Nogales-Fernández Esteban , Jiménez-Cortegana Carlos , Sánchez-León María L. , Silva-Romeiro Silvia , Flores-Campos Rocío , Carnicero-González Fernando , Ríos-Herranz Eduardo , de la Cruz-Vicente Fátima , Rodríguez-García Guillermo , Fernández-Álvarez Rubén , Martínez-Banaclocha Natividad , Gumà-Padrò Josep , Gómez-Codina José , Salar-Silvestre Antonio , Rodríguez-Abreu Delvys , Gálvez-Carvajal Laura , Labrador Jorge , Guirado-Risueño María , Provencio-Pulla Mariano , Sánchez-Beato Margarita , Marylene Lejeune , Álvaro-Naranjo Tomás , Casanova-Espinosa María , Rueda-Domínguez Antonio , Sánchez-Margalet Víctor , de la Cruz-Merino Luis
Published: February 2024

Background

Diffuse large B cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma worldwide. DLBCL is an aggressive disease that can be cured with upfront standard chemoimmunotherapy schedules. However, in approximately 35-40% of the patients DLBCL relapses, and therefore, especially in this setting, the search for new prognostic and predictive biomarkers is an urgent need. Natural killer (NK) are effector cells characterized by playing an important role in antitumor immunity due to their cytotoxic capacity and a subset of circulating NK that express CD8 have a higher cytotoxic function. In this substudy of the R2-GDP-GOTEL trial, we have evaluated blood CD8+ NK cells as a predictor of treatment response and survival in relapsed/refractory (R/R) DLBCL patients.

Methods

78 patients received the R2-GDP schedule in the phase II trial. Blood samples were analyzed by flow cytometry. Statistical analyses were carried out in order to identify the prognostic potential of CD8+ NKs at baseline in R/R DLBCL patients.

Results

Our results showed that the number of circulating CD8+ NKs in R/R DLBCL patients were lower than in healthy donors, and it did not change during and after treatment. Nevertheless, the level of blood CD8+ NKs at baseline was associated with complete responses in patients with R/R DLBCL. In addition, we also demonstrated that CD8+ NKs levels have potential prognostic value in terms of overall survival in R/R DLBCL patients.

Conclusion

CD8+ NKs represent a new biomarker with prediction and prognosis potential to be considered in the clinical management of patients with R/R DLBCL.

Clinical trial registration

https://www.clinicaltrialsregister.eu/ctr-search/search?query=2014-001620-29 EudraCT, ID:2014-001620-29.
Bi-specific autoantigen-T cell engagers as targeted immunotherapy for autoreactive B cell depletion in autoimmune diseases
Authors: Perico Luca , Casiraghi Federica , Sônego Fabiane , Todeschini Marta , Corna Daniela , Cerullo Domenico , Pezzotta Anna , Isnard-Petit Patricia , Faravelli Silvia , Forneris Federico , Thiam Kader , Benigni Ariela , Remuzzi Giuseppe
Published: February 2024

Introduction

In autoimmune diseases, autoreactive B cells comprise only the 0.1-0.5% of total circulating B cells. However, current first-line treatments rely on non-specific and general suppression of the immune system, exposing patients to severe side effects. For this reason, identification of targeted therapies for autoimmune diseases is an unmet clinical need.

Methods

Here, we designed a novel class of immunotherapeutic molecules, Bi-specific AutoAntigen-T cell Engagers (BiAATEs), as a potential approach for targeting the small subset of autoreactive B cells. To test this approach, we focused on a prototype autoimmune disease of the kidney, membranous nephropathy (MN), in which phospholipase A2 receptor (PLA2R) serves as primary nephritogenic antigen. Specifically, we developed a BiAATE consisting of the immunodominant Cysteine-Rich (CysR) domain of PLA2R and the single-chain variable fragment (scFv) of an antibody against the T cell antigen CD3, connected by a small flexible linker.

Results

BiAATE creates an immunological synapse between autoreactive B cells bearing an CysR-specific surface Ig+ and T cells. Ex vivo, the BiAATE successfully induced T cell-dependent depletion of PLA2R-specific B cells isolated form MN patients, sparing normal B cells. Systemic administration of BiAATE to mice transgenic for human CD3 reduced anti-PLA2R antibody levels following active immunization with PLA2R.

Discussion

Should this approach be confirmed for other autoimmune diseases, BiAATEs could represent a promising off-the-shelf therapy for precision medicine in virtually all antibody-mediated autoimmune diseases for which the pathogenic autoantigen is known, leading to a paradigm shift in the treatment of these diseases.
Benchmarking glycoform-resolved affinity separation – mass spectrometry assays for studying FcγRIIIa binding
Authors: Gstöttner Christoph , Lippold Steffen , Hook Michaela , Yang Feng , Haberger Markus , Wuhrer Manfred , Falck David , Schlothauer Tilman , Domínguez-Vega Elena
Published: February 2024

The antibody- FcγRIIIa interaction triggers key immunological responses such as antibody dependent cellular cytotoxicity (ADCC), making it highly important for therapeutic mAbs. Due to the direct glycan-glycan interaction with FcγRIIIa receptor, differences in antibody glycosylation can drastically influence the binding affinity. Understanding the differential binding of mAb glycoforms is a very important, yet challenging task due to the co-existence of multiple glycoforms in a sample. Affinity liquid chromatography (AC) and affinity capillary electrophoresis (ACE) hyphenated with mass spectrometry (MS) can provide glycoform-resolved affinity profiles of proteins based on their differences in either dissociation (AC) or equilibrium (ACE) constants. To cross-validate the affinity ranking provided by these complementary novel approaches, both techniques were benchmarked using the same FcγRIIIa constructs. Both approaches were able to assess the mAb – FcγRIIIa interaction in a glycoform selective manner and showed a clear increase in binding for fully versus hemi-fucosylated mAbs. Also, other features, such as increasing affinity with elevated galactosylation or the binding affinity for high mannose glycoforms were consistent. We further applied these approaches to assess the binding towards the F158 allotype of FcγRIIIa, which was not reported before. The FcγRIIIa F158 allotype showed a very similar profile compared to the V158 receptor with the strongest increase in binding due to afucosylation and only a slight increase in binding with additional galactosylation. Both techniques showed a decrease of the binding affinity for high mannose glycoforms for FcγRIIIa F158 compared to the V158 variant. Overall, both approaches provided very comparable results in line with orthogonal methods proving the capabilities of separation-based affinity approaches to study FcγR binding of antibody glycoforms.
An exploratory computational analysis in mice brain networks of widespread epileptic seizure onset locations along with potential strategies for effective intervention and propagation control
Authors: Courson Juliette , Quoy Mathias , Timofeeva Yulia , Manos Thanos
Published: February 2024

Mean-field models have been developed to replicate key features of epileptic seizure dynamics. However, the precise mechanisms and the role of the brain area responsible for seizure onset and propagation remain incompletely understood. In this study, we employ computational methods within The Virtual Brain framework and the Epileptor model to explore how the location and connectivity of an Epileptogenic Zone (EZ) in a mouse brain are related to focal seizures (seizures that start in one brain area and may or may not remain localized), with a specific focus on the hippocampal region known for its association with epileptic seizures. We then devise computational strategies to confine seizures (prevent widespread propagation), simulating medical-like treatments such as tissue resection and the application of an anti-seizure drugs or neurostimulation to suppress hyperexcitability. Through selectively removing (blocking) specific connections informed by the structural connectome and graph network measurements or by locally reducing outgoing connection weights of EZ areas, we demonstrate that seizures can be kept constrained around the EZ region. We successfully identified the minimal connections necessary to prevent widespread seizures, with a particular focus on minimizing surgical or medical intervention while simultaneously preserving the original structural connectivity and maximizing brain functionality.
C9orf72 gene networks in the human brain correlate with cortical thickness in C9-FTD and implicate vulnerable cell types
Authors: Broce Iris J. , Sirkis Daniel W. , Nillo Ryan M. , Bonham Luke W. , Lee Suzee E. , Miller Bruce L. , Castruita Patricia A. , Sturm Virginia E. , Sugrue Leo S. , Desikan Rahul S. , Yokoyama Jennifer S.
Published: February 2024

Introduction

A hexanucleotide repeat expansion (HRE) intronic to chromosome 9 open reading frame 72 (C9orf72) is recognized as the most common genetic cause of amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and ALS-FTD. Identifying genes that show similar regional co-expression patterns to C9orf72 may help identify novel gene targets and biological mechanisms that mediate selective vulnerability to ALS and FTD pathogenesis.

Methods

We leveraged mRNA expression data in healthy brain from the Allen Human Brain Atlas to evaluate C9orf72 co-expression patterns. To do this, we correlated average C9orf72 expression values in 51 regions across different anatomical divisions (cortex, subcortex, and cerebellum) with average gene expression values for 15,633 protein-coding genes, including 54 genes known to be associated with ALS, FTD, or ALS-FTD. We then performed imaging transcriptomic analyses to evaluate whether the identified C9orf72 co-expressed genes correlated with patterns of cortical thickness in symptomatic C9orf72 pathogenic HRE carriers (n = 19) compared to controls (n = 23). Lastly, we explored whether genes with significant C9orf72 imaging transcriptomic correlations (i.e., “C9orf72 imaging transcriptomic network”) were enriched in specific cell populations in the brain and enriched for specific biological and molecular pathways.

Results

A total of 2,120 genes showed an anatomical distribution of gene expression in the brain similar to C9orf72 and significantly correlated with patterns of cortical thickness in C9orf72 HRE carriers. This C9orf72 imaging transcriptomic network was differentially expressed in cell populations previously implicated in ALS and FTD, including layer 5b cells, cholinergic neurons in the spinal cord and brainstem and medium spiny neurons of the striatum, and was enriched for biological and molecular pathways associated with protein ubiquitination, autophagy, cellular response to DNA damage, endoplasmic reticulum to Golgi vesicle-mediated transport, among others.

Conclusion

Considered together, we identified a network of C9orf72 associated genes that may influence selective regional and cell-type-specific vulnerabilities in ALS/FTD.
Efficacy of polyethylene glycol loxenatide for type 2 diabetes mellitus patients: a systematic review and meta-analysis
Authors: Liu Yibo , Ma Wenjing , Fu Hui , Zhang Zhe , Yin Yanyan , Wang Yongchun , Liu Wei , Yu Shaohong , Zhang Zhongwen
Published: February 2024

Objective: Some studies have proved that polyethylene glycol loxenatide (PEG-Loxe) has significant effects on controlling blood glucose and body weight in patients with type 2 diabetes mellitus (T2DM), but there is still some controversy over the improvement of blood lipid profiles (BLP) and blood pressure (BP), and more evidences are needed to verify such effects. Therefore, this study was conducted to provide a comprehensive evaluation of the efficacy of PEG-Loxe in improving blood glucose (BG), BLP, BP, body mass index (BMI), and body weight (BW) in patients with T2DM for clinical reference.

Methods: Randomized controlled trials (RCT) in which PEG-Loxe was applied to treat T2DM were retrieved by searching PubMed, Cochrane Library, Embase, Medline, Scopus, Web of Science, China National Knowledge Infrastructure, China Scientific Journal, Wanfang Data, and SinoMed databases. Outcome measures included BG, BLP, BP, BMI, and BW. RevMan 5.3 software was used to perform data analysis.

Results: Eighteen trials were identified involving 2,166 patients. In experimental group 1,260 patients received PEG-Loxe alone or with other hypoglycemic agents, while in control group 906 patients received placebo or other hypoglycemic agents. In the overall analysis, PEG-Loxe significantly reduced the levels of glycosylated hemoglobin (HbA1c), fasting plasma glucose (FPG), 2-h postprandial blood glucose (2-h PBG), BMI, and BW compared with control group. However, it had no obvious effect on total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), systolic blood pressure (SBP), and diastolic blood pressure (DBP).

Conclusion: PEG-Loxe has better hypoglycemic effects compared with placebo in patients with T2DM, but could not significantly improved TG, LDL-C, HDL-C, SBP, and DBP. And the combination of conventional hypoglycemic drugs (CHD) and PEG-Loxe could more effectively improve the levels of HbA1c, FPG, 2-h PBG, TC, TG, BMI, and BW compared with CHD in T2DM patients.

Systematic Review Registration:www.inplasy.com, identifier INPLASY202350106
In silico models of the macromolecular NaV1.5-KIR2.1 complex
Authors: Stary-Weinzinger Anna
Published: February 2024

In cardiac cells, the expression of the cardiac voltage-gated Na+ channel (NaV1.5) is reciprocally regulated with the inward rectifying K+ channel (KIR2.1). These channels can form macromolecular complexes that pre-assemble early during forward trafficking (transport to the cell membrane). In this study, we present in silico 3D models of NaV1.5-KIR2.1, generated by rigid-body protein-protein docking programs and deep learning-based AlphaFold-Multimer software. Modeling revealed that the two channels could physically interact with each other along the entire transmembrane region. Structural mapping of disease-associated mutations revealed a hotspot at this interface with several trafficking-deficient variants in close proximity. Thus, examining the role of disease-causing variants is important not only in isolated channels but also in the context of macromolecular complexes. These findings may contribute to a better understanding of the life-threatening cardiovascular diseases underlying KIR2.1 and NaV1.5 malfunctions.
“Genome-wide identification of bZIP gene family in Pearl millet and transcriptional profiling under abiotic stress, phytohormonal treatments; and functional characterization of PgbZIP9”
Authors: Jha Deepak Kumar , Chanwala Jeky , Barla Preeti , Dey Nrisingha
Published: February 2024

Abiotic stresses are major constraints in crop production, and are accountable for more than half of the total crop loss. Plants overcome these environmental stresses using coordinated activities of transcription factors and phytohormones. Pearl millet an important C4 cereal plant having high nutritional value and climate resilient features is grown in marginal lands of Africa and South-East Asia including India. Among several transcription factors, the basic leucine zipper (bZIP) is an important TF family associated with diverse biological functions in plants. In this study, we have identified 98 bZIP family members (PgbZIP) in pearl millet. Phylogenetic analysis divided these PgbZIP genes into twelve groups (A-I, S, U and X). Motif analysis has shown that all the PgbZIP proteins possess conserved bZIP domains and the exon-intron organization revealed conserved structural features among the identified genes. Cis-element analysis, RNA-seq data analysis, and real-time expression analysis of PgbZIP genes suggested the potential role of selected PgbZIP genes in growth/development and abiotic stress responses in pearl millet. Expression profiling of selected PgbZIPs under various phytohormones (ABA, SA and MeJA) treatment showed differential expression patterns of PgbZIP genes. Further, PgbZIP9, a homolog of AtABI5 was found to localize in the nucleus and modulate gene expression in pearl millet under stresses. Our present findings provide a better understanding of bZIP genes in pearl millet and lay a good foundation for the further functional characterization of multi-stress tolerant PgbZIP genes, which could become efficient tools for crop improvement.