bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Three-Dimensional Frontier: Advancements in MSC Spheroids and Their Implications for Breast Cancer and Personalized Regenerative Therapies
Authors: Smolinska Veronika , Harsanyi Stefan , Bohac Martin , Danisovic Lubos
Published: December 2023

To more accurately replicate the in vivo three-dimensional (3D) mesenchymal stem cell (MSC) niche and enhance cellular phenotypes for superior in vivo treatments, MSC functionalization through in vitro 3D culture approaches has gained attention. The organization of MSCs in 3D spheroids results in altered cell shape, cytoskeleton rearrangement, and polarization. Investigations have revealed that the survival and secretory capability of MSCs are positively impacted by moderate hypoxia within the inner zones of MSC spheroids. The spheroid hypoxic microenvironment enhances the production of angiogenic and anti-apoptotic molecules, including HGF, VEGF, and FGF-2. Furthermore, it upregulates the expression of hypoxia-adaptive molecules such as CXCL12 and HIF-1, inhibiting MSC death. The current review focuses on the latest developments in fundamental and translational research concerning three-dimensional MSC systems. This emphasis extends to the primary benefits and potential applications of MSC spheroids, particularly in the context of breast cancer and customized regenerative therapies.
Mitochondrial Dynamics in Pulmonary Hypertension
Authors: Santos Ed Wilson , Khatoon Subika , Di Mise Annarita , Zheng Yun-Min , Wang Yong-Xiao
Published: December 2023

Mitochondria are essential organelles for energy production, calcium homeostasis, redox signaling, and other cellular responses involved in pulmonary vascular biology and disease processes. Mitochondrial homeostasis depends on a balance in mitochondrial fusion and fission (dynamics). Mitochondrial dynamics are regulated by a viable circadian clock. Hypoxia and nicotine exposure can cause dysfunctions in mitochondrial dynamics, increases in mitochondrial reactive oxygen species generation and calcium concentration, and decreases in ATP production. These mitochondrial changes contribute significantly to pulmonary vascular oxidative stress, inflammatory responses, contractile dysfunction, pathologic remodeling, and eventually pulmonary hypertension. In this review article, therefore, we primarily summarize recent advances in basic, translational, and clinical studies of circadian roles in mitochondrial metabolism in the pulmonary vasculature. This knowledge may not only be crucial to fully understanding the development of pulmonary hypertension, but also greatly help to create new therapeutic strategies for treating this devastating disease and other related pulmonary disorders.
Synthesis and Antimicrobial Activity of 3D Micro–Nanostructured Diatom Biosilica Coated by Epitaxially Growing Ag-AgCl Hybrid Nanoparticles
Authors: Bekissanova Zhanar , Railean Viorica , Wojtczak Izabela , Brzozowska Weronika , Trykowski Grzegorz , Ospanova Alyiya , Sprynskyy Myroslav
Published: December 2023

The 3D (three-dimensional) micro–nanostructured diatom biosilica obtained from cultivated diatoms was used as a support to immobilize epitaxially growing AgCl-Ag hybrid nanoparticles ((Ag-AgCl)NPs) for the synthesis of nanocomposites with antimicrobial properties. The prepared composites that contained epitaxially grown (Ag-AgCl)NPs were investigated in terms of their morphological and structural characteristics, elemental and mineral composition, crystalline forms, zeta potential, and photoluminescence properties using a variety of instrumental methods including SEM (scanning electron microscopy), TEM (transmission electron microscopy), EDX (energy-dispersive X-ray spectroscopy), XRD (X-ray powder diffraction), zeta-potential measurement, and photoluminescence spectroscopy. The content of (AgCl-Ag)NPs in the hybrid composites amounted to 4.6 mg/g and 8.4 mg/g with AgClNPs/AgNPs ratios as a percentage of 86/14 and 51/49, respectively. Hybrid nanoparticles were evenly dispersed with a dominant size of 5 to 25 nm in composite with an amount of 8.4 mg/g of silver. The average size of the nanoparticles was 7.5 nm; also, there were nanoparticles with a size of 1–2 nm and particles that were 20–40 nm. The synthesis of (Ag-AgCl)NPs and their potential mechanism were studied. The MIC (the minimum inhibitory concentration method) approach was used to investigate the antimicrobial activity against microorganisms Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus. The nanocomposites containing (Ag-AgCl)NPs and natural diatom biosilica showed resistance to bacterial strains from the American Type Cultures Collection and clinical isolates (diabetic foot infection and wound isolates).
Complex Modal Characteristic Analysis of a Tensegrity Robotic Fish’s Body Waves
Authors: Chen Bingxing , Zhang Jie , Meng Qiuxu , Dong Hui , Jiang Hongzhou
Published: December 2023

A bionic robotic fish based on compliant structure can excite the natural modes of vibration, thereby mimicking the body waves of real fish to generate thrust and realize undulate propulsion. The fish body wave is a result of the fish body’s mechanical characteristics interacting with the surrounding fluid. Thoroughly analyzing the complex modal characteristics in such robotic fish contributes to a better understanding of the locomotion behavior, consequently enhancing the swimming performance. Therefore, the complex orthogonal decomposition (COD) method is used in this article. The traveling index is used to quantitatively describe the difference between the real and imaginary modes of the fish body wave. It is defined as the reciprocal of the condition number between the real and imaginary components. After introducing the BCF (body and/or caudal fin) the fish’s body wave curves and the COD method, the structural design and parameter configuration of the tensegrity robotic fish are introduced. The complex modal characteristics of the tensegrity robotic fish and real fish are analyzed. The results show that their traveling indexes are close, with two similar complex mode shapes. Subsequently, the relationship between the traveling index and swimming performance is expressed using indicators reflecting linear correlation (correlation coefficient (Rc) and p value). Based on this correlation, a preliminary optimization strategy for the traveling index is proposed, with the potential to improve the swimming performance of the robotic fish.
Asterias forbesi-Inspired SERS Substrates for Wide-Range Detection of Uric Acid
Authors: Park Hyunjun , Chai Kyunghwan , Kim Woochang , Park Joohyung , Lee Wonseok , Park Jinsung
Published: December 2023

Uric acid (UA), the final metabolite of purine, is primarily excreted through urine to maintain an appropriate concentration in the bloodstream. However, any malfunction in this process can lead to complications due to either deficiency or excess amount of UA. Hence, the development of a sensor platform with a wide-range detection is crucial. To realize this, we fabricated a surface-enhanced Raman spectroscopy (SERS) substrate inspired by a type of starfish with numerous protrusions, Asterias forbesi. The Asterias forbesi-inspired SERS (AF-SERS) substrate utilized an Au@Ag nanostructure and gold nanoparticles to mimic the leg and protrusion morphology of the starfish. This substrate exhibited excellent Raman performance due to numerous hotspots, demonstrating outstanding stability, reproducibility, and repeatability. In laboratory settings, we successfully detected UA down to a concentration of 1.16 nM (limit of detection) and demonstrated selectivity against various metabolites. In the experiments designed for real-world application, the AF-SERS substrate detected a broad range of UA concentrations, covering deficiencies and excesses, in both serum and urine samples. These results underscore the potential of the developed AF-SERS substrate as a practical detection platform for UA in real-world applications.
A Portable and Disposable Electrochemical Sensor Utilizing Laser-Scribed Graphene for Rapid SARS-CoV-2 Detection
Authors: Wang Runzhong , Zhu Bicheng , Young Paul , Luo Yu , Taylor John , Cameron Alan J. , Squire Christopher J. , Travas-Sejdic Jadranka
Published: December 2023

The COVID-19 pandemic caused by the virus SARS-CoV-2 was the greatest global threat to human health in the last three years. The most widely used methodologies for the diagnosis of COVID-19 are quantitative reverse transcription polymerase chain reaction (RT-qPCR) and rapid antigen tests (RATs). PCR is time-consuming and requires specialized instrumentation operated by skilled personnel. In contrast, RATs can be used in-home or at point-of-care but are less sensitive, leading to a higher rate of false negative results. In this work, we describe the development of a disposable, electrochemical, and laser-scribed graphene-based biosensor strips for COVID-19 detection that exploits a split-ester bond ligase system (termed ‘EsterLigase’) for immobilization of a virus-specific nanobody to maintain the out-of-plane orientation of the probe to ensure the efficacy of the probe-target recognition process. An anti-spike VHH E nanobody, genetically fused with the EsterLigase domain, was used as the specific probe for the spike receptor-binding domain (SP-RBD) protein as the target. The recognition between the two was measured by the change in the charge transfer resistance determined by fitting the electrochemical impedance spectroscopy (EIS) spectra. The developed LSG-based biosensor achieved a linear detection range for the SP-RBD from 150 pM to 15 nM with a sensitivity of 0.0866 [log(M)]−1 and a limit of detection (LOD) of 7.68 pM.
Multifunctional Electrospun Nanofibers for Biosensing and Biomedical Engineering Applications
Authors: Chen Zhou , Guan Mengdi , Bian Yi , Yin Xichen
Published: December 2023

Nanotechnology is experiencing unprecedented developments, leading to the advancement of functional nanomaterials. The properties that stand out include remarkable porosity, high-specific surface area, excellent loading capacity, easy modification, and low cost make electrospun nanofibers. In the biomedical field, especially in biosensors, they exhibit amazing potential. This review introduces the principle of electrospinning, describes several structures and biomaterials of electrospun nanofibers used for biomedicine, and summarizes the applications of this technology in biosensors and other biomedical applications. In addition, the technical challenges and limitations of electrospinning for biomedicine are discussed; however, more research work is needed to elucidate its full potential.
Vanillic Acid Ameliorates Demyelination in a Cuprizone-Induced Multiple Sclerosis Rat Model: Possible Underlying Mechanisms
Authors: Safwat Sally M. , El Tohamy Mahmoud , Aboonq Moutasem Salih , Alrehaili Amaal , Assinnari Ahmad A. , Bahashwan Abdulrahman S. , ElGendy Ahmed A. , Hussein Abdelaziz M.
Published: December 2023

Objective: To investigate the effect of vanillic acid (VA) on a Cuprizone (Cup) demyelinating rat model and the mechanisms behind such effect. Methods: Thirty adult male Sprague Dawley (SD) rats were randomly divided into three groups: control, Cuprizone, and VA groups. Cuprizone was administrated at a dose of 450 mg/kg per day orally via gastric gavage for 5 weeks. The nerve conduction velocity (NCV) was studied in an isolated sciatic nerve, and then the sciatic nerve was isolated for histopathological examination, electron microscope examination, immunohistochemical staining, and biochemical and PCR assay. The level of IL17 was detected using ELISA, while the antioxidant genes Nrf2, HO-1 expression at the level of mRNA, expression of the myelin basic protein (MBP), interferon-gamma factor (INF)-γ and tumor necrosis factor (TNF)-α, and apoptotic marker (caspase-3) were measured using immunohistochemistry in the sciatic nerve. Results: There was a significant reduction in NCV in Cup compared to normal rats (p < 0.001), which was markedly improved in the VA group (p < 0.001). EM and histopathological examination revealed significant demyelination and deterioration of the sciatic nerve fibers with significant improvement in the VA group. The level of IL17 as well as the expression of INF-γ and caspase-3 were significantly increased with a significant reduction in the expression of MBP, Nrf2, and HO-1 in the sciatic nerve (p < 0.01), and VA treatment significantly improved the studied parameters (p < 0.01). Conclusion: The current study demonstrated a neuroprotective effect for VA against the Cup-induced demyelinating rat model. This effect might be precipitated by the inhibition of inflammation, oxidative stress, and apoptosis.
Activity of Protein Kinase A in the Frontal Cortex in Schizophrenia
Authors: Sahay Smita , Henkel Nicholas Daniel , Vargas Christina Flora-Anabelle , McCullumsmith Robert Erne , O’Donovan Sinead Marie
Published: December 2023

Schizophrenia is a serious cognitive disorder characterized by disruptions in neurotransmission, a process requiring the coordination of multiple kinase-mediated signaling events. Evidence suggests that the observed deficits in schizophrenia may be due to imbalances in kinase activity that propagate through an intracellular signaling network. Specifically, 3′-5′-cyclic adenosine monophosphate (cAMP)-associated signaling pathways are coupled to the activation of neurotransmitter receptors and modulate cellular functions through the activation of protein kinase A (PKA), an enzyme whose function is altered in the frontal cortex in schizophrenia. In this study, we measured the activity of PKA in human postmortem anterior cingulate cortex (ACC) and dorsolateral prefrontal cortex (DLPFC) tissue from schizophrenia and age- and sex-matched control subjects. No significant differences in PKA activity were observed in male and female individuals in either brain region; however, correlation analyses indicated that PKA activity in the ACC may be influenced by tissue pH in all subjects and by age and tissue pH in females. Our data provide novel insights into the function of PKA in the ACC and DLPFC in schizophrenia.
The Distant Molecular Effects on the Brain by Cancer Treatment
Authors: Demos-Davies Kimberly , Lawrence Jessica , Ferreira Clara , Seelig Davis
Published: December 2023

Cancer survivors experience cancer-related cognitive impairment (CRCI) secondary to treatment. Chemotherapy and radiation therapy independently contribute to cognitive dysfunction; however, the underlying mechanisms leading to dysfunction remain unclear. We characterized brain gene expression changes in a mouse model of CRCI to identify the mechanistic underpinnings. Eleven-to-twelve-week-old SKH1 mice were treated with doxorubicin (DOX), hindlimb radiation (RT), concurrent hindlimb radiation and doxorubicin (DOX-RT), or no treatment (control). Sixteen days following treatment, gene expression was measured from murine brains using the NanoString nCounter® glial profiling panel. Gene expression was normalized and compared between groups. No two groups shared the same expression pattern, and only Gnb1 and Srpr were upregulated in multiple treatment groups. Brains from DOX-treated mice had upregulated Atf2, Atp5b, Gnb1, Rad23b, and Srpr and downregulated Sirt5 expression compared to control brains. Brains from RT-treated mice demonstrated increased Abcg2 and Fgf2 and decreased C1qa and C1qb expression compared to control brains. Brains from DOX-RT-treated mice had upregulated Adar, E2f3, Erlec1, Gnb1, Srpr, Vim, and Pdgfra expression and downregulated Rock2 and Inpp5f expression compared to control brains. The gene expression changes demonstrated here highlight roles for neuronal transmission and oxidative stress in the pathogenesis of doxorubicin-related CRCI and inflammation in RT-related CRCI.
The Lexicon and Morphosyntax of Child Spanish as Predictors of Inhibition
Authors: Grinstead John , Sorine Nina
Published: December 2023

This study investigates the relationships between lexical development and inhibition, as well as morphosyntax and inhibition, in typically developing monolingual Spanish-speaking children. Recent studies of the relationship between lexical development and inhibition suggest that, as the size of the lexicon increases, so does inhibitory ability. However, the relationship between grammar and inhibition seems more controversial. The work distinguishing the relationships between inhibition and lexicon vs. grammar have been carried out in English, which has relatively impoverished inflectional morphology. Because the relationships considered in the literature are hypothetically not language-particular to English, but rather claims about cognition in general, we would expect to find that they also hold in other languages, including languages with richer morphology, such as Spanish. These considerations led us to ask the following: are expressive and receptive measures of the lexicon and morphosyntax predictive of typically developing monolingual child Spanish-speakers’ inhibitory ability? A sample of 82 monolingual, typically developing Spanish-speaking children in Mexico City were tested with 5 lexical measures, 4 morphosyntax measures, and the Flanker Task measure of inhibition. Results showed that all lexical and morphosyntactic variables correlated significantly with Flanker (p < 0.01), except for Number of Different Words (NDW), calculated on the spontaneous production sample. Therefore, inhibition is predicted by lexical development in child Spanish. Additionally, an ever-increasing set of competitor morphological forms requires an ever-increasing inhibitory ability as well.
The Father’s Part: A Pilot Evaluation of a Father-Centered Family Intervention Group in Child and Adolescent Psychiatry
Authors: Mestermann Stefan , Kleinöder Jonas Markus , Arndt Marie , Krämer Josef , Eichler Anna , Kratz Oliver
Published: December 2023

Changes in parental roles have renewed the focus on a father’s involvement in an offspring’s psychological development. However, fathers are still under-represented in family research. There are only a few structured father-centered intervention programs in child and adolescent psychiatry. In a German population sample, a pilot father-centered family intervention program with n = 16 participants, conducted in person (n = 8) and online (n = 8), in a child and adolescent psychiatry inpatient/day clinic setting was evaluated by comparing paternal stress, PSE, and child-rated paternal competence in a pre–post design. Participating fathers showed significant decreases in child-related parenting stress (presence: p = 0.042, online: p = 0.047) and significant increases in PSE (p = 0.006/0.012). Parent-related stress and child-rated paternal competence were unaffected (p = 0.108/0.171; p = 0.167/0.101), while small-to-medium effect size measures pointed in the direction of our hypothesis (d = 0.48/0.36; d = 0.37/0.50). Participant satisfaction was higher in person than online (p = 0.008). As social and biological fathers have important influences on child and adolescent well-being and development, they should be included more frequently in prevention and intervention programs. Fathers seem to benefit from gender-specific intervention programs with regard to stress reduction, as well as experiencing competence- and PSE-increasing effects.
Effect of Achievement Motivation and Self-Efficacy on General Well-Being among Students at Normal Universities in Ningxia: The Mediating Role of Time Management
Authors: Dong Jingyi , Hassan Norlizah Che , Hassan Aminuddin Bin , Chen Dan , Guo Wei
Published: December 2023

General well-being is a positive evaluation of one’s mental health, which is an important topic in mental health. General well-being is fundamental to the positive development of young people. A thorough understanding of the factors that influence happiness have not yet been developed for students at normal universities in Ningxia Province in China. This study examined the mediation effects of time management on the relationship between achievement motivation, self-efficacy, and general well-being among students at normal universities in Ningxia Province in China. Using a random sampling, 163 participants (68 males and 95 females) completed the Achievement Motivation Scale (AMS), General Self-efficacy Scale (GSES), Time Management Questionnaire (TMQ) and General Well-being Scale (GWBS). Achievement motivation, self-efficacy, and time management were positively correlated with general well-being. The mediation effects of time management on the relationships between achievement motivation, self-efficacy, and general wellbeing (0.169 and 0.447) were demonstrated. These results add to the nuanced relationship between self-efficacy, achievement motivation, and general well-being. According to the Achievement Motivation Theory and the Self-efficacy Theory, this study reveals the role of achievement motivation, self-efficacy on general well-being and the mediating effect of time management in the relationship between achievement motivation, self-efficacy, and general well-being. According to the findings, the school can carry out a series of lectures and tutoring activities to enhance students’ achievement motivation, self-efficacy, time management, and general well-being.
Industry 4.0 Maturity of General Contractors: An In-Depth Case Study Analysis
Authors: Das Priyadarshini , Perera Srinath , Senaratne Sepani , Osei-Kyei Robert
Published: December 2023

Industry 4.0 technologies have business process re-engineering capabilities that can radically improve process performance and increase the effectiveness of communication and collaboration between actors. Despite the significant emphasis on technology adoption, the slow uptake rate can be attributed to ignoring nontechnical aspects, such as systemic change concerning people and processes. There are ample Industry 4.0 maturity models in the literature; however, a common criticism of maturity models lies in their applicability. This paper attempts to address this criticism by presenting two case studies where maturity assessments for general contractors were conducted to understand how ready they were to adopt to Industry 4.0 and what they could do to improve their current readiness. This paper aims to answer the following research questions: (1) Can the Industry 4.0 maturity modelling literature be applied in an organisational context for a construction general contractor? (2) Do construction general contractors relate to such an assessment? To answer the research questions, a comprehensive assessment of the Industry 4.0 maturity of two general contractors was conducted, thereby intending to support their strategic planning and systematise their transformation in Industry 4.0. The richness of the findings lies in the detailed understanding of the organisation’s current Industry 4.0 capabilities and future plans, thereby establishing the applicability of the maturity attributes identified from the literature and confirming whether the managers of a construction organisation can relate to them.
Urban Density and Land Leverage: Market Value Breakdown for Energy-Efficient Assets
Authors: Canesi Rubina , Marella Giuliano
Published: December 2023

A real estate asset comprises land and improvements. The proportions of these components vary over time and across locations. Notably, the land value component is consistent over time, unaffected by depreciation. Consequently, the weight of land value in determining the overall asset value is crucial, particularly in those improvements that are highly sensitive to depreciation, such as energy-efficient buildings. While several studies have explored the relationship between energy-efficient building consumption and urban density, there is currently a research gap concerning the relationship between land value and the value of efficient improvements built on it. Before investigating this potential relationship, it is imperative to preliminary examine any possible correlations between land values and land density. To verify this correlation, we captured the “Land Leverage” of a real estate property by calculating the ratio between the value of the land and the total value of the real estate property and correlating it with the allowable density. Our analysis of the Land Leverage (LL) trend in a restricted development area over a ten-year period demonstrates that LL increases with the level of permitted density in a neighborhood. This evidence will serve as the foundation to verify whether Land leverage, through urban-densification strategies, might be a pivotal factor in driving the values of energy-efficient assets.
Diatomaceous Soils and Advances in Geotechnical Engineering—Part II
Authors: Zuluaga-Astudillo Daniel , Slebi-Acevedo Carlos , Ruge Juan Carlos , Camacho-Tauta Javier , Caicedo-Hormaza Bernardo
Published: December 2023

In the geotechnical area, advances in diatomaceous soil research are laconic and mainly oriented towards understanding the primary soil response (typical characterization methods) considering observation and experience (failures in construction processes or unexpected laboratory results) more than following a scientific method. Coincident results have been evidenced in the correspondence between the content of frustules, the effective friction angle and the water retention capacity. However, the variables and processes that control some mechanical behaviors have yet to be documented in the literature, such as the level of fracturing of the frustules and its relation with interlocked behavior, compressibility and shear strength. In addition to the bibliographic background, SEM microscopy records are presented. These facilitate the understanding of the described phenomena. The images highlight the level of deterioration, the environment, the morphology and the pores present in diatoms of different origins. The morphology (as a function of the species) of intact or fractured frustules (depending on the stress record) affects the mechanical responses and volumetric variations of the diatomaceous deposits. Furthermore, this review presents some emerging research lines in diatomaceous soils, such as the subjection of structures to geotechnical centrifuge conditions, some constitutive models and the criteria for developing water retention curves.
Preparation and Characterization of a Novel Lightweight Bio-Degradable Lignocellulosic Porous Molding Material
Authors: Feng Yongshun , Pan Xin , Qiao Hui , Zhuang Xiaowei
Published: December 2023

Wood is an abundant biomaterial and widely used in construction and furniture. Timber processing produces large amounts of residues and byproducts, which are of low value. In this study, we proposed a new strategy for the recycle of wood residues to prepare a wood porous molding material. A hydrated thermochemical grinding process followed by high-temperature and high-pressure refining was developed to convert wood powder into high-viscosity suspension. Lignocellulosic raw materials, including pine wood, beech wood, and bamboo, were compared with different grinding time. A porous material without the addition of synthetic adhesive was obtained with a density in the range of 0.28–0.67 g/cm3. The porous molding material was characterized based on fiber morphology, volume, and porosity and mechanical performance. Pores of different sizes were distributed in the samples randomly after curing and drying. The wood’s own bindings were released through the hydrated thermochemical grinding process. The porous sample made from bamboo with a grinding time of 6 h showed a high Young’s modulus (681.1 MPa), compactness (166.8 N/Sec), and hardness (517.6 N). Woody materials were more readily made into moldings since most of the cellulose crystal structure remained intact. The wood porous moldings are fully composed of lignocellulosic components and easy to recycle. This porous green material has great potential to be applied to insulation, ceiling, cabinet, and packaging.
Indicators for Takt Production Performance Assessment—A Conceptual Study
Authors: Keskiniva Kimmo , Saari Arto , Junnonen Juha-Matti
Published: December 2023

This conceptual study aims to produce rough analysis methods and visualizations for production data (formatted in time, location, and work) that can be collected from construction sites that utilize takt production. The scope is on creating methods for evaluating the soundness of the takt plan and its execution. Relevant production literature regarding takt production management and data collection are utilized in the production of the methods and visualizations. However, only imaginary production data are utilized in this study to keep the indicators as simplified and clear as possible. A total of seven indicators with varying levels of novelty are provided in the study. The proposed indicators emphasize punctual adherence to the takt schedule, homogenous production pace, avoiding trade overlapping in locations, steady work in process, and coherent short and long-term production targets. Both as-planned and as-built perspectives are considered. The proposed indicators are argued to be valuable for production management and research and development processes since they provide status information and document the progression of the production for later indicators purposes. This study also acts as a foundation for further empirical studies regarding takt production data utilization.
Effects of Power Optimizer Application in a Building-Integrated Photovoltaic System According to Shade Conditions
Authors: Eum Jiyoung , Park Seunghwan , Choi Hyun-Jung
Published: December 2023

A building-integrated photovoltaic (BIPV) system produces power using photovoltaic (PV) modules as building exterior materials, whose architectural performance serves the same functions as those of existing building materials. Most relevant studies targeted general PV modules used in the building-mounted and -attached types. This study aims to integrate the building elevation-type BIPV system and exterior materials to secure both exterior material performance and PV electrical performance by embedding a power optimization device in an integrated system of BIPV modules and exterior materials. Thus, the advantages of economy, safety, aesthetics, and ease of maintenance can be achieved. In this study, experiments were conducted on elevation-type BIPV modules with and without a power optimizer, that is, a DC/DC converter, under various shade conditions, and the power loss rate of the BIPV system was analyzed. The power optimizer-equipped BIPV system was experimentally observed to have a PV power-loss rate approximately 2–3 times lower than that of the BIPV system without a power optimizer when the shade ratio of one module was approximately 10–75%. This exterior material-integrated BIPV-specific power optimization device reduces dependence on fossil fuels for power production and improves energy sustainability, contributing to the spread of zero-energy buildings and carbon neutrality.
The Effects of River Torrents and Debris on Historic Masonry Vaulted Arch Bridges
Authors: Van Bogaert Philippe , De Backer Hans
Published: December 2023

The carrying capacity for vertical loads of well-maintained masonry arch bridges is reasonably high. This might not be the case for horizontal loads, the effects of which have not been the subject of extensive research aside from seismic occurrences. Arch bridges crossing rivers are subjected to sudden horizontal loads, due to river torrents, carrying debris from higher grounds. The magnitude of these horizontal loads is similar to those of coastal waves and debris; however, their effect on these structures has yet to be explored in detail. The narrow and high Devil’s Bridge across the Arda River (BG) and the wide, low Candia Viaduct across the Sesia River (I) were chosen as examples. Both are strongly exposed to fast-washing flow in the river during spring. FE simulations show that the impact of the rapidly rising river water influences the general stability, while the effect of debris mainly causes local damage. The results exhibit that tall, slender masonry arch structures fail due to the brittle fracture of the material, followed by the shear failure of a pier body. In contrast, lower and wider viaducts fail due to exaggerated tensile cracking in the upstream parts of a pier and the associated increasing pressure at its downstream parts.
Behaviour of Slender Hybrid Rubberised Concrete Double Skin Tubular Columns under Eccentric Loading
Authors: Khusru Shovona , Thambiratnam David P. , Elchalakani Mohamed , Fawzia Sabrina
Published: December 2023

Rubberised concrete, utilised as infill material within single- or double-skin confinements, has emerged as a sustainable solution, offering improved ductility in structures. Past studies have indicated promising results regarding the axial response of hybrid columns comprising filament wound exterior tubes, rubberised concrete infill, and steel interior tubes. This paper investigates the response of such hybrid columns under eccentric compression using validated numerical techniques. An extensive parametric study is conducted to explore the effects of load eccentricity, rubber percentage, concrete strength, and steel tube strength. Results show that despite credible increases in rubber percentage and load eccentricity, the columns have reasonably good performance. The findings facilitate the prediction of the eccentric behaviour of these hybrid columns across varying rubber percentages, confirming its viability for practical applications under realistic eccentric load conditions. The results further affirm the suitability of this hybrid column in scenarios that necessitate higher ductility.
Local Thermal Comfort and Physiological Responses in Uniform Environments
Authors: Zhong Xianzhun , Yu Hang , Tang Yin , Mao Huice , Zhang Kege
Published: December 2023

The thermal perception of different body parts can vary greatly throughout the human body and have different influences on overall thermal sensation and comfort. Various personal comfort systems (PCS) have been developed to stimulate local body parts for the purpose of enhancing human thermal comfort, yet the most effective body parts for intervention remain undetermined. Therefore, a series of climate chamber experiments under five uniform environments with three sets of suits were conducted in this study. The results showed that the head, chest, belly, and hands tended to feel no cooler than overall in cooler environments, but arms and legs felt generally no warmer than overall in warmer environments. The head, trunk and upper arms were more likely to be the comfort-dominant body parts. Additionally, the upper arms and upper back expected temperature regulation measures the most under non-neutral environments, thus they seem to be the two most needed and effective targeted body parts that a PCS could be applied to. The skin temperature and thermal sensation of limbs were more sensitive to indoor air temperatures than those of the torso. However, variations in the skin temperature of the head, chest, upper back, and calves had the strongest correlation with overall sensation vote changes. The above results and conclusions can not only serve as the basis for the future studies of local thermal comfort, but also provide theoretical guidance for the design of future PCS products.
Behavior of FRP-Retrofitted Wall-like RC Columns after Preloading to Simulate In-Service Conditions
Authors: Elsanadedy Hussein , Abbas Husain , Almusallam Tarek , Al-Salloum Yousef
Published: December 2023

In the Middle East, wall-like reinforced concrete (RC) columns are a common choice in multistory buildings. Sometimes, these columns need axial retrofitting for increased load capacity. In practice, unstrengthened columns bear their load, and if retrofitting is necessary, the load is released before the upgrade—unlike in past research studies that overlooked this real-world scenario. This study aimed to investigate the response of preloaded wall-like RC columns after being retrofitted using different configurations. In the experimental campaign, two half-scale columns were cast and axially loaded to 80% of their capacity, and the load was then totally released. After that, these specimens were strengthened with two different schemes, and hence, they were concentrically loaded until failure. In both schemes, the section shape was not modified. The first scheme comprised wrapping carbon FRP (fiber-reinforced polymer) sheets together with near-surface mounted (NSM) steel rebars. However, the second technique was composed of wrapping glass FRP (GFRP) sheets together with NSM steel rebars and bolted steel plates. The second scheme was found to be superior to the first one due to the extra confinement provided by the bolted steel plates. This scheme improved the peak load, stiffness, and dissipated energy by 115%, 75%, and 524%, respectively. Other than the testing campaign, nonlinear numerical modeling was undertaken to examine the behavior of tested specimens. The models were utilized to conduct a parametric study, exploring the influence of the percentage of preloading and the amount of load release on the response of columns strengthened with the second scheme.
Prioritizing Indicators for Material Selection in Prefabricated Wooden Construction
Authors: Cabral Matheus R. , Blanchet Pierre
Published: December 2023

Material selection in buildings profoundly affects project success, encompassing durability, maintenance, customer satisfaction, production systems, lifecycle, usage, environment, and costs. Yet, there is a need for further research on indicators for choosing materials in prefabricated buildings. Therefore, this study’s main objective was to identify the indicators (criteria and sub-criteria) for selecting materials for prefabricated wooden construction and, subsequently, categorize these criteria and sub-criteria based on the perspective of industry professionals. To achieve this goal, three phases were carried out. First, a literature review was conducted to identify potential criteria for choosing structural and envelope materials in wooden prefabricated buildings. Second, a pilot survey was conducted in Canada and the United States to classify the priority order of the criteria obtained from the literature based on professionals’ opinions. Finally, Monte Carlo simulations were conducted with different iterations (1000, 10,000, and 100,000) using the data obtained from the previous phase to improve decision-making and classification processes. For the indicators to select materials, the literature review identified seven main criteria: performance properties, green materials, energy efficiency, circular economy, site conditions and material logistics, standards, and social impact. These criteria contained a total of 25 sub-criteria. The pilot survey data analysis demonstrated that the performance properties, site conditions and material logistics, and social impact criteria were consistently prioritized. The critical sub-criteria identified were fire resistance, watertightness, local availability, occupant health, and safety and protection. For the Monte Calo simulations, the predictions aligned with the pilot study, enhancing the robustness of the results.
An Analysis of the Factors Influencing Cadmium Removal in Aquatic Environments by Chlorella vulgaris-Derived Solids
Authors: Sufian Jafar , Babaakbari Sari Mohammad , Marchelli Filippo , Fiori Luca , Avanes Armen , Moradi Salahedin
Published: December 2023

Chlorella vulgaris is an inexpensive microalga that could be employed for environmental remediation, but further investigations are needed to assess its suitability and optimal treatment methodology. With this aim in mind, this study focused on the raw biomass and the biochar and hydrochar obtained from it, analyzing their physicochemical properties and testing them to capture cadmium from an aqueous environment. The adsorption/absorption tests assessed the effect of adsorbent dosage, pH, Cd concentration, and contact time, and the results were analyzed through a structural equation model. Biochar and hydrochar performed similarly and better than the raw biomass, with the highest Cd removal observed at an adsorbent dosage of 0.8 g L−1, an initial concentration of Cd solution of 30 mg L−1, a pH of 6, and a contact time of 30 min. The adsorption isotherm data for Cd could be well-described by the Langmuir and Temkin models. The results from the structural equation modeling revealed that the variables material type, dosage, and concentration all contributed to Cd removal in water, with time mediating these effects.
The Role of tRNA-Centered Translational Regulatory Mechanisms in Cancer
Authors: Shi Yuanjian , Feng Yipeng , Wang Qinglin , Dong Gaochao , Xia Wenjie , Jiang Feng
Published: December 2023

Cancer is a leading cause of morbidity and mortality worldwide. While numerous factors have been identified as contributing to the development of malignancy, our understanding of the mechanisms involved remains limited. Early cancer detection and the development of effective treatments are therefore critical areas of research. One class of molecules that play a crucial role in the transmission of genetic information are transfer RNAs (tRNAs), which are the most abundant RNA molecules in the human transcriptome. Dysregulated synthesis of tRNAs directly results in translation disorders and diseases, including cancer. Moreover, various types of tRNA modifications and the enzymes responsible for these modifications have been implicated in tumor biology. Furthermore, alterations in tRNA modification can impact tRNA stability, and impaired stability can prompt the cleavage of tRNAs into smaller fragments known as tRNA fragments (tRFs). Initially believed to be random byproducts lacking any physiological function, tRFs have now been redefined as non-coding RNA molecules with distinct roles in regulating RNA stability, translation, target gene expression, and other biological processes. In this review, we present recent findings on translational regulatory models centered around tRNAs in tumors, providing a deeper understanding of tumorigenesis and suggesting new directions for cancer treatment.
The PedAL/EuPAL Project: A Global Initiative to Address the Unmet Medical Needs of Pediatric Patients with Relapsed or Refractory Acute Myeloid Leukemia
Authors: Ceolin Valeria , Ishimaru Sae , Karol Seth E. , Bautista Francisco , Goemans Bianca Frederika , Gueguen Gwenaëlle , Willemse Marieke , Di Laurenzio Laura , Lukin Jennifer , van Tinteren Harm , Locatelli Franco , Petit Arnaud , Tomizawa Daisuke , Norton Alice , Kaspers Gertjan , Reinhardt Dirk , Tasian Sarah K. , Nichols Gwen , Kolb Edward Anders , Zwaan Christian Michel , Cooper Todd Michael
Published: December 2023

The prognosis of children with acute myeloid leukemia (AML) has improved incrementally over the last few decades. However, at relapse, overall survival (OS) is approximately 40–50% and is even lower for patients with chemo-refractory disease. Effective and less toxic therapies are urgently needed for these children. The Pediatric Acute Leukemia (PedAL) program is a strategic global initiative that aims to overcome the obstacles in treating children with relapsed/refractory acute leukemia and is supported by the Leukemia and Lymphoma Society in collaboration with the Children’s Oncology Group, the Innovative Therapies for Children with Cancer consortium, and the European Pediatric Acute Leukemia (EuPAL) foundation, amongst others. In Europe, the study is set up as a complex clinical trial with a stratification approach to allocate patients to sub-trials of targeted inhibitors at relapse and employing harmonized response and safety definitions across sub-trials. The PedAL/EuPAL international collaboration aims to determine new standards of care for AML in a first and second relapse, using biology-based selection markers for treatment stratification, and deliver essential data to move drugs to front-line pediatric AML studies. An overview of potential treatment targets in pediatric AML, focused on drugs that are planned to be included in the PedAL/EuPAL project, is provided in this manuscript.
Cyclooxygenase-2 Blockade Is Crucial to Restore Natural Killer Cell Activity before Anti-CTLA-4 Therapy against High-Grade Serous Ovarian Cancer
Authors: Gómez-Valenzuela Fernán , Wichmann Ignacio , Suárez Felipe , Kato Sumie , Ossandón Enrique , Hermoso Marcela , Fernández Elmer A. , Cuello Mauricio A.
Published: December 2023

Chronic inflammation influences the tumor immune microenvironment (TIME) in high-grade serous ovarian cancer (HGSOC). Specifically, cyclooxygenase-2 (COX-2) overexpression promotes cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) expression. Notably, elevated COX-2 levels in the TIME have been associated with reduced response to anti-CTLA-4 immunotherapy. However, the precise impact of COX-2, encoded by PTGS2, on the immune profile remains unknown. To address this, we performed an integrated bioinformatics analysis using data from the HGSOC cohorts (TCGA-OV, n = 368; Australian cohort AOCS, n = 80; GSE26193, n = 62; and GSE30161, n = 45). Employing Gene Set Variation Analysis (GSVA), MIXTURE and Ecotyper cell deconvolution algorithms, we concluded that COX-2 was linked to immune cell ecosystems associated with shorter survival, cell dysfunction and lower NK cell effector cytotoxicity capacity. Next, we validated these results by characterizing circulating NK cells from HGSOC patients through flow cytometry and cytotoxic assays while undergoing COX-2 and CTLA-4 blockade. The blockade of COX-2 improved the cytotoxic capacity of NK cells against HGSOC cell lines. Our findings underscore the relevance of COX-2 in shaping the TIME and suggest its potential as a prognostic indicator and therapeutic target. Increased COX-2 expression may hamper the effectivity of immunotherapies that require NK cell effector function. These results provide a foundation for experimental validation and clinical trials investigating combined therapies targeting COX-2 and CTLA-4 in HGSOC.
Synergistic Suppression of NF1 Malignant Peripheral Nerve Sheath Tumor Cell Growth in Culture and Orthotopic Xenografts by Combinational Treatment with Statin and Prodrug Farnesyltransferase Inhibitor PAMAM G4 Dendrimers
Authors: Reiners John J. , Mathieu Patricia A. , Gargano Mary , George Irene , Shen Yimin , Callaghan John F. , Borch Richard F. , Mattingly Raymond R.
Published: December 2023

Neurofibromatosis type 1 (NF1) is a disorder in which RAS is constitutively activated due to the loss of the Ras-GTPase-activating activity of neurofibromin. RAS must be prenylated (i.e., farnesylated or geranylgeranylated) to traffic and function properly. Previous studies showed that the anti-growth properties of farnesyl monophosphate prodrug farnesyltransferase inhibitors (FTIs) on human NF1 malignant peripheral nerve sheath tumor (MPNST) cells are potentiated by co-treatment with lovastatin. Unfortunately, such prodrug FTIs have poor aqueous solubility. In this study, we synthesized a series of prodrug FTI polyamidoamine generation 4 (PAMAM G4) dendrimers that compete with farnesyl pyrophosphate for farnesyltransferase (Ftase) and assessed their effects on human NF1 MPNST S462TY cells. The prodrug 3-tert-butylfarnesyl monophosphate FTI-dendrimer (i.e., IG 2) exhibited improved aqueous solubility. Concentrations of IG 2 and lovastatin (as low as 0.1 μM) having little to no effect when used singularly synergistically suppressed cell proliferation, colony formation, and induced N-RAS, RAP1A, and RAB5A deprenylation when used in combination. Combinational treatment had no additive or synergistic effects on the proliferation/viability of immortalized normal rat Schwann cells, primary rat hepatocytes, or normal human mammary epithelial MCF10A cells. Combinational, but not singular, in vivo treatment markedly suppressed the growth of S462TY xenografts established in the sciatic nerves of immune-deficient mice. Hence, prodrug farnesyl monophosphate FTIs can be rendered water-soluble by conjugation to PAMAM G4 dendrimers and exhibit potent anti-tumor activity when combined with clinically achievable statin concentrations.