bluuebunny/crossref_metadata_2025 huggingface dataset:
The interplay between non-esterified fatty acids and bovine peroxisome proliferator-activated receptors: results of an in vitro hybrid approach
Authors: Busato Sebastiano , Bionaz Massimo
Published: August 2020

Background

In dairy cows circulating non-esterified fatty acids (NEFA) increase early post-partum while liver and other tissues undergo adaptation to greater lipid metabolism, mainly regulated by peroxisome proliferator-activated receptors (PPAR). PPAR are activated by fatty acids (FA), but it remains to be demonstrated that circulating NEFA or dietary FA activate bovine PPAR. We hypothesized that circulating NEFA and dietary FA activate PPAR in dairy cows.

Methods

The dose-response activation of PPAR by NEFA or dietary FA was assessed using HP300e digital dispenser and luciferase reporter in several bovine cell types. Cells were treated with blood plasma isolated from Jersey cows before and after parturition, NEFA isolated from the blood plasma, FA released from lipoproteins using milk lipoprotein lipase (LPL), and palmitic acid (C16:0). Effect on each PPAR isotype was assessed using specific synthetic inhibitors.

Results

NEFA isolated from blood serum activate PPAR linearly up to ~ 4-fold at 400 μmol/L in MAC-T cells but had cytotoxic effect. Addition of albumin to the culture media decreases cytotoxic effects of NEFA but also PPAR activation by ~ 2-fold. Treating cells with serum from peripartum cows reveals that much of the PPAR activation can be explained by the amount of NEFA in the serum (R2 = 0.91) and that the response to serum NEFA follows a quadratic tendency, with peak activation around 1.4 mmol/L. Analysis of PPAR activation by serum in MAC-T, BFH-12 and BPAEC cells revealed that most of the activation is explained by the activity of PPARδ and PPARγ, but not PPARα. Palmitic acid activated PPAR when added in culture media or blood serum but the activation was limited to PPARδ and PPARα and the response was nil in serum from post-partum cows. The addition of LPL to the serum increased > 1.5-fold PPAR activation.

Conclusion

Our results support dose-dependent activation of PPAR by circulating NEFA in bovine, specifically δ and γ isotypes. Data also support the possibility of increasing PPAR activation by dietary FA; however, this nutrigenomics approach maybe only effective in pre-partum but not post-partum cows.
National Estimates of hospital emergency department visits due to acute injuries associated with hookah smoking, United States, 2011–2019
Authors: Inyang Naa A. , Chang Joanne T. , Wang Baoguang
Published: August 2020

Introduction

Hookah (also known as waterpipe) smoking is associated with acute adverse health effects such as vomiting and fainting, symptoms related to carbon monoxide poisoning, and decreased pulmonary function, however, national estimates of hookah-related acute injuries are not currently available in the scientific literature. This study provides national estimates of United States hospital emergency department visits due to hookah-related acute injuries.

Methods

We analyzed 2011–2019 data from the National Electronic Injury Surveillance System to calculate national estimates of emergency department visits due to hookah-related acute injuries. National Electronic Injury Surveillance System data were gathered from approximately 100 United States hospitals selected as a probability sample of approximately 5000 hospitals with emergency departments. Each case contains information abstracted from all emergency department records involving injuries associated with consumer products. All individuals admitted to emergency departments who sustained hookah-related acute injuries were included in the study.

Results

During 2011–2019, an estimated 1371 (95% confidence interval: 505–2283) United States hospital emergency department visits were related to hookah-related acute injuries. The most common injuries were sustained from dizziness/light-headedness and syncopal episodes (54.8%), followed by burns (41.5%). Young adults aged 18–24 years accounted for 66.8% of hookah-related acute injuries admitted to United States emergency departments.

Conclusions

This study provides national estimates of hospital emergency department visits due to hookah-related acute injuries. We found that hookah smoking related AIs mostly occurred among young adults. Study findings may inform public health policy and educational intervention efforts to prevent these events and complement other acute injury surveillance systems, such as the National Poison Data System.
18F-FDG PET/CT in patients with polymyositis/dermatomyositis: correlation with serum muscle enzymes
Authors: Arai-Okuda Hanae , Norikane Takashi , Yamamoto Yuka , Mitamura Katsuya , Fujimoto Kengo , Takami Yasukage , Wakiya Risa , Nakashima Shusaku , Dobashi Hiroaki , Nishiyama Yoshihiro
Published: August 2020

Background

Muscle enzymes are the major noninvasive diagnostic parameters useful in polymyositis/dermatomyositis (PM/DM). Few studies have yet correlated findings on 18F-FDG PET with disease activity in patients with PM/DM.

Purpose

We evaluated 18F-FDG muscle uptake in patients with PM/DM compared with non-muscular diseases and correlated the results with serum muscle enzymes.

Methods

A total of 28 patients with untreated PM/DM and 28 control patients with non-muscular diseases were examined with 18F-FDG PET/CT. 18F-FDG uptake was evaluated in 9 proximal skeletal muscle regions bilaterally. The uptake was scored as follows: 0 = less than that of the mediastinal blood vessels, 1 = greater than or equal to that of the mediastinal blood vessels, and 2 = greater than or equal to that of the liver. A score 1 or 2 was considered positive. The mean and maximum standardized uptake values (SUV) were calculated in each muscle and were averaged for all muscle regions. PET findings were correlated with serum muscle enzymes.

Results

18F-FDG uptake was observed in 82% of patients with PM/DM and 7% of control patients. The number of positive regions, total score, mean SUVmean, and mean SUVmax in patients with PM/DM were significantly higher than those in the control patients (all P < 0.001). The total score of 2 was the best cut-off value that could discriminate patients with PM/DM from control patients. The total score, mean SUVmean, and mean SUVmax showed significant correlations with creatine kinase (P = 0.047, 0.002, 0.010, respectively) and aldolase (P = 0.036, 0.005, 0.038, respectively).

Conclusion

18F-FDG PET/CT using visual and SUV methods demonstrated its usefulness by discriminating PM/DM from non-muscular diseases and correlating with serum muscle enzymes in patients with PM/DM.
Microbiome of the Southwestern Atlantic invasive scleractinian coral, Tubastraea tagusensis
Authors: Zanotti Aline Aparecida , Gregoracci Gustavo Bueno , Capel Katia Cristina Cruz , Kitahara Marcelo Visentini
Published: August 2020

Background

Commonly known as sun-coral,Tubastraea tagusensisis an azooxanthellate scleractinian coral that successfully invaded the Southwestern Atlantic causing significant seascape changes. Today it is reported to over 3500 km along the Brazilian coast, with several rocky shores displaying high substrate coverage. Apart from its singular invasiveness capacity, the documentation and, therefore, understanding of the role of symbiotic microorganisms in the sun-coral invasion is still scarce. However, in general, the broad and constant relationship between corals and microorganisms led to the development of co-evolution hypotheses. As such, it has been shown that the microbial community responds to environmental factors, adjustment of the holobiont, adapting its microbiome, and improving the hosts’ fitness in a short space of time. Here we describe the microbial community (i.e. Bacteria) associated with sun-coral larvae and adult colonies from a locality displaying a high invasion development.

Results

The usage of high throughput sequencing indicates a great diversity of Bacteria associated withT. tagusensis, withCyanobacteria,Proteobacteria,Bacteroidetes,Actinobacteria,Planctomycetes,andFirmicutescorresponding to the majority of the microbiome in all samples. However,T. tagusensis’microbial core consists of only eight genera for colonies, and, within them, three are also present in the sequenced larvae. Overall, the microbiome from colonies sampled at different depths did not show significant differences. The microbiome of the larvae suggests a partial vertical transfer of the microbial core in this species.

Conclusion

Although diverse, the microbiome core of adultTubastraea tagusensisis composed of only eight genera, of which three are transferred from the mother colony to their larvae. The remaining bacteria genera are acquired from the seawater, indicating that they might play a role in the host fitness and, therefore, facilitate the sun-coral invasion in the Southwestern Atlantic.
Time-domain simulations of turbulence effects on the aerodynamic flutter of long-span bridges
Authors: Liu Shuqian , Cai C. S. , Han Yan
Published: August 2020

Aerodynamic flutter instability has been a major concern for long-span flexible bridges, such as suspension and cable-stayed bridges, subjected to wind actions that result in the so-called self-excited forces. Though turbulence effects on bridge flutter have been studied in the last few decades, its true effects remain a debate due to the limitation of previous wind tunnel facilities, such as using turbulence scales that are too small in these experiments. In this paper, the characterizations of self-excited forces are presented in both the frequency-domain and in the time-domain. Then, the flutter analysis is conducted under both smooth flow and turbulent flow in order to investigate the effect of wind turbulence on the flutter instability. The effect of wind turbulence is directly modeled in the time-domain in order to avoid the complicated random parametric excitation analysis of the equation of motion used in previous studies. By comparing the results of different turbulence intensities with that of the smooth flow, it is found that the turbulence has a stabilizing effect on bridge flutter. The turbulence can change the vibration patterns and weaken the spatial vibration correlation to some extent. As a result, the critical flutter velocity can be increased by 5% to 10% over that under smooth flow.
Beyond clicks and downloads: a call for a more comprehensive approach to measuring mobile-health app engagement
Authors: O'Brien Heather L. , Morton Emma , Kampen Andrea , Barnes Steven J. , Michalak Erin E.
Published: August 2020

Downloading a mobile health (m-health) app on your smartphone does not mean you will ever use it. Telling another person about an app does not mean you like it. Using an online intervention does not mean it has had an impact on your well-being. Yet we consistently rely on downloads, clicks, ‘likes’ and other usage and popularity metrics to measure m-health app engagement. Doing so misses the complexity of how people perceive and use m-health apps in everyday life to manage mental health conditions. This article questions commonly used behavioural metrics of engagement in mental health research and care, and proposes a more comprehensive approach to measuring in-app engagement.
Linking language features to clinical symptoms and multimodal imaging in individuals at clinical high risk for psychosis
Authors: Haas S. S. , Doucet G. E. , Garg S. , Herrera S. N. , Sarac C. , Bilgrami Z. R. , Shaik R. B. , Corcoran C. M.
Published: August 2020

Background.

Abnormalities in the semantic and syntactic organization of speech have been reported in individuals at clinical high-risk (CHR) for psychosis. The current study seeks to examine whether such abnormalities are associated with changes in brain structure and functional connectivity in CHR individuals.

Methods.

Automated natural language processing analysis was applied to speech samples obtained from 46 CHR and 22 healthy individuals. Brain structural and resting-state functional imaging data were also acquired from all participants. Sparse canonical correlation analysis (sCCA) was used to ascertain patterns of covariation between linguistic features, clinical symptoms, and measures of brain morphometry and functional connectivity related to the language network.

Results.

In CHR individuals, we found a significant mode of covariation between linguistic and clinical features (r = 0.73; p = 0.003), with negative symptoms and bizarre thinking covarying mostly with measures of syntactic complexity. In the entire sample, separate sCCAs identified a single mode of covariation linking linguistic features with brain morphometry (r = 0.65; p = 0.05) and resting-state network connectivity (r = 0.63; p = 0.01). In both models, semantic and syntactic features covaried with brain structural and functional connectivity measures of the language network. However, the contribution of diagnosis to both models was negligible.

Conclusions.

Syntactic complexity appeared sensitive to prodromal symptoms in CHR individuals while the patterns of brain-language covariation seemed preserved. Further studies in larger samples are required to establish the reproducibility of these findings.
Features of Cytologically Indeterminate Molecularly Benign Nodules Treated With Surgery
Authors: Endo Mayumi , Porter Kyle , Long Clarine , Azaryan Irina , Phay John E , Ringel Matthew D , Sipos Jennifer A , Nabhan Fadi
Published: August 2020

Background

Most cytologically indeterminate thyroid nodules (ITNs) with benign molecular testing are not surgically removed. The data on clinical outcomes of these nodules are limited.

Methods

We retrospectively analyzed all ITNs where molecular testing was performed either with the Afirma gene expression classifier or Afirma gene sequencing classifier between 2011 and 2018 at a single institution.

Results

Thirty-eight out of 289 molecularly benign ITNs were ultimately resected. The most common reason for surgery was compressive symptoms (39%). In multivariable modeling, patients aged <40 years, nodules ≥3 cm, presence of an Afirma suspicious nodule other than the index nodule, and compressive symptoms were associated with higher surgery rates with hazard ratios for surgery of 3.5 (P < 0.001), 3.2 (P < 0.001), 16.8 (P < 0.001), and 7.31 (P < 0.001), respectively. Of resected nodules, 5 were malignant. False-negative rate (FNR) was 1.7%, presuming all unresected nodules were truly benign and 13.2% restricting analysis to resected cases. The FNR was significantly higher in nodules with a high-risk sonographic appearance for cancer (American Thyroid Association high-risk classification and American College of Radiology Thyroid Imaging Reporting and Data Systems score of 5) compared with nodules with all other sonographic categories (11.8% vs 1.1%; P = 0.03 and 11.1% vs 1.1%; P = 0.02, respectively).

Conclusions

Younger age, larger nodule size, presence of an Afirma suspicious nodule other than the index nodule, and compressive symptoms were associated with a higher rate of surgery. The FNR of benign Afirma was significantly higher in nodules with high-risk sonographic features.
The role of the α-tubulin acetyltransferase αTAT1 in the DNA damage response
Authors: Ryu Na Mi , Kim Jung Min
Published: August 2020

Lysine 40 acetylation of α-tubulin (Ac-α-tubulin), catalyzed by the acetyltransferase αTAT1, marks stabilized microtubules. Recently, there is growing evidence to suggest crosstalk between the DNA damage response (DDR) and microtubule organization; we therefore investigated whether αTAT1 is involved in the DDR. Following treatment with DNA-damaging agents, increased levels of Ac-α-tubulin were detected. We also observed significant induction of Ac-α-tubulin after depletion of DNA repair proteins, suggesting that αTAT1 is positively regulated in response to DNA damage. Intriguingly, αTAT1 depletion decreased DNA damage-induced replication protein A (RPA) phosphorylation and foci formation. Moreover, DNA damage-induced cell cycle arrest was significantly delayed in αTAT1-depleted cells, indicating defective checkpoint activation. The checkpoint defects seen upon αTAT1 deficiency were restored by expression of wild-type αTAT1, but not by αTAT1-D157N (a catalytically inactive αTAT1), indicating that the role of αTAT1 in the DDR is dependent on enzymatic activity. Furthermore, αTAT1-depleted direct repeat GFP (DR-GFP) U2OS cells had a significant decrease in the frequency of homologous recombination repair. Collectively, our results suggest that αTAT1 may play an essential role in DNA damage checkpoints and DNA repair through its acetyltransferase activity.
Broadening the functional and evolutionary understanding of postnatal neurogenesis using reptilian models
Authors: LaDage Lara D.
Published: August 2020

The production of new neurons in the brains of adult animals was first identified by Altman and Das in 1965, but it was not until the late 20th century when methods for visualizing new neuron production improved that there was a dramatic increase in research on neurogenesis in the adult brain. We now know that adult neurogenesis is a ubiquitous process that occurs across a wide range of taxonomic groups. This process has largely been studied in mammals; however, there are notable differences between mammals and other taxonomic groups in how, why and where new neuron production occurs. This Review will begin by describing the processes of adult neurogenesis in reptiles and identifying the similarities and differences in these processes between reptiles and model rodent species. Further, this Review underscores the importance of appreciating how wild-caught animals vary in neurogenic properties compared with laboratory-reared animals and how this can be used to broaden the functional and evolutionary understanding of why and how new neurons are produced in the adult brain. Studying variation in neural processes across taxonomic groups provides an evolutionary context to adult neurogenesis while also advancing our overall understanding of neurogenesis and brain plasticity.
Total energy
Authors: Polischuk Vladimir
Published: August 2020

The training manual sets out General issues of energy systems, fundamentals of converting various forms of energy (thermal, nuclear, hydraulic, solar, wind, geothermal, etc.) into electrical energy, explained the device, principles of operation, modes of operation of heat and power generating equipment and the main issues related to the transmission and distribution of electric energy.
Designed for students enrolled in the direction "Electro - and heat power engineering".
Material management of railway transport
Authors: Cevelev Aleksandr
Published: August 2020

In the monograph reviewed the development of the inventory management of railway transport in the new economic environment of market economy.
According to the results of theoretical research, innovative and production potential of the supply system of railway transport the main directions and methods of transformation of the restructuring process under the corporate changes of JSC "RZD", positioned value system of the logistics of railway transportation, and developed a classification model used logistical resources.
Evaluation of activity of structural divisions of Russian Railways supply is proposed to be viewed through an integrated and comprehensive approach to the development of systems of balanced indicators of supply and prompt handling of material resources, the implementation of which allows to distribute the strategic objectives of the company "Russian Railways" activities in the system of logistics of the Railways and also to involve in economic circulation of excessive and unused inventories of material and technical resources and efficiently reallocate them among enterprises at the site of the railway.
Recommendations for the implementation of the developed algorithms and models are long term in nature and are based on the concept of logistics management and improve the business processes of the logistics system.
Will be useful for managers and specialists of directorates of logistics of Russian Railways supply, undergraduates and graduate students interested in the economy of railway transport.
Olympic tourism: organizational and economic aspects and impacts on host destination
Authors: Vetitnev Aleksandr , Bobina Nadezhda
Published: August 2020

The monograph reflects the results of years of research on the issue of Olympic tourism.
For professionals in the field of tourism, heads of tourism administrations, marketers, University students enrolled in field of study 43.03.02 Tourism and other areas of tourist profile.
Unemployment and Digital Public Goods Contribution
Authors: Kummer Michael , Slivko Olga , Zhang Xiaoquan (Michael)
Published: August 2020

Economic crises have a harmful effect on employment. However, whereas the resulting loss of jobs has been shown to have many negative consequences for the affected individuals, it may also push them into new activities, such as provision of service to their communities. In this paper, we show how individuals engage in socially useful activities after an increase in unemployment. Specifically, we document increased online content generation at Wikipedia, the world’s largest user-generated knowledge repository. Leveraging German district-level and European country-level unemployment data, we analyze the relationship between the economic crisis in 2008–2010 and contributions to Wikipedia. We find increased socially valuable activity in the form of knowledge acquisition and contributions to Wikipedia. For German districts, we observe an increase in the rate of content generation on Wikipedia in more severely affected districts. These effects are even stronger at the European country level. Our findings suggest that public goods provision increases as a positive side effect of economic crises. We stress that similar patterns could apply to other digital content platforms. Under the backdrop that the potential value of the outcome of online volunteering and its societal impact is expected to grow drastically in the next years, we show that platforms could benefit from negative economic conditions in attracting volunteers. Moreover, in the coming years, the rapid development of artificial intelligence will call for a rise of online volunteering platforms. Therefore, the potential value of the outcome of online volunteering and its societal impact is expected to grow drastically in the next years.
Soft tissue management in open tibial shaft fractures
Authors: Garner Matthew R. , Warner Stephen J. , Heiner Jacob A. , Kim Yesul T. , Agel Julie
Published: August 2020

Aims

To compare results of institutional preferences with regard to treatment of soft tissues in the setting of open tibial shaft fractures.

Methods

We present a retrospective review of open tibial shaft fractures at two high-volume level 1 trauma centres with differing practices with regard to the acute management of soft tissues. Site 1 attempts acute primary closure, while site 2 prefers delayed closure/coverage. Comparisons include percentage of primary closure, number of surgical procedures until definitive closure, percentage requiring soft tissue coverage, and percentage of 90-day wound complication.

Results

Overall, there were 219 patients at site 1 and 282 patients at site 2. Differences in rates of acute wound closure were seen (168 (78%) at site 1 vs 101 (36%) at site 2). A mean of 1.5 procedures for definitive closure was seen at site 1 compared to 3.4 at site 2. No differences were seen in complication, nonunion, or amputation rates. Similar results were seen in a sub-analysis of type III injuries.

Conclusion

Comparing outcomes of open tibial shaft fractures at two institutions with different rates initial wound management, no differences were seen in 90-day wound complications, nonunion rates, or need for amputation. Attempted acute closure resulted in a lower number of planned secondary procedures when compared with planned delayed closure. Providers should consider either acute closure or delayed coverage based on the injury characteristics, surgeon preference and institutional resources without concern that the decision at the time of index surgery will lead to an increased risk of complication. Cite this article: Bone Joint Open 2020;1-8:481–487.
Species diversity, population structure, and regeneration status of trees in Fakim Wildlife Sanctuary, Nagaland, Northeast India
Authors: Ao Aosanen , Changkija Sapu , S. K. Tripathi
Published: August 2020

Abstract. Ao A, Changkija S, Tripathi SK. 2020. Species diversity, population structure, and regeneration status of trees in Fakim Wildlife Sanctuary, Nagaland, Northeast India. Biodiversitas 21: 2777-2785. The plant species composition and diversity represent the overall health of the forest ecosystems and provide useful insight on forest conservation, and the species regeneration is important in assessing the potential of the forests to serve the society on sustained basis. This present study aimed to assess the species diversity, population structure, and regeneration status of the trees in temperate forest of Fakim Wildlife Sanctuary, Nagaland, Northeast India. Tree species composition, population structure and regeneration status were analyzed by randomly establishing eight plots of 0.1 ha within the sanctuary. Density, basal area, abundance, evenness, and other diversity indices were calculated for mature trees with girth at breast height (GBH) ≥ 30 cm. A total of 60 tree species belonging to 40 genera and 27 families were recorded. The most dominant families recorded were Lauraceae and Rosaceae. The total tree density recorded from the forest was 432.5 individuals ha-1 with a basal area 42.8 m2 ha-1. Cinnamomum zeylanicum and Lithocarpus pachyphyllus had the highest density with 17.58 individuals ha-1 and Lithocarpus pachyphyllus had the highest basal area with 2.64 m2 ha-1. The species with the highest IVI was Betula alnoides (17.13). Girth class of 30-60 cm had the highest density and basal area with 280 individuals ha-1 and 20.7 m2 ha-1. The diversity indices such as Shannon-Wiener index, Simpson index, Pielou evenness index, and Margalef species richness index were 3.90, 0.93, 0.92, and 11.59, respectively in the wildlife sanctuary. The present study showed that 41% of tree species exhibited good regeneration status, 31% as fair regeneration, 8% as poor regeneration and 20% had no regeneration. The present data will be useful to understand the current status of tree species and will serve as baseline information to the forest department, policymakers and conservationists to develop management plans for the conservation of priority species in the area.
Root detection by resistivity imaging and physiological activity with the dead-end trench on three clones of cocoa (Theobroma cacao)
Authors: Nailil Canggih Nailil Maghfiroh , EKA TARWACA SUSILA PUTRA  , ENDANG SRI DEWI HS
Published: August 2020

Abstract. Maghfiroh CN, Putra ETS, Dewi HSES. 2020. Root detection by resistivity imaging and physiological activity with the dead-end trench on three clones of cocoa (Theobroma cacao). Biodiversitas 21: 2794-2803. Indonesia is one of the largest cocoa production countries in Southeast Asia, but has low average productivity (982 kg/ha) based on Indonesian Plantation Statistics (2017). Productivity increase effort by giving dead-end trenches that applied to collect organic material, accommodate surface runoff sediments, prevent erosion, and maintain nutrient availability. Dead-end trench had an impact on root cutting and improve rooting. Clone is very influential on cocoa (Theobroma cacao L.) yield. The superior clones recommended are clones RCC-70, RCC-71, and KKM-22, which have high productivity and are resistant to pests and diseases. The objectives of this research were (i) to detect the presence of roots by resistivity imaging (ii) to study the effects of dead-end trench application on physiological activities of cocoa leaves and yields of three clones (RCC-70, RCC-71, and KKM-22); (iii) to determine which cocoa clone (s) performed a significant yield increase with the application of dead-end trench. Research was conducted in August 2018-April 2019 at PT. Pagilaran cocoa plantation in North Segayung Production Unit, subdistrict Tulis, Batang, Central Java. The experiment was arranged in a randomized complete design with two factors and three blocks as replications. The first factor was dead-end trench application (with or without dead-end trench application) and the second factor was cocoa clones (RCC-70, RCC-71, and KKM-22). This study showed that dead-end trench applications affected plant roots based on the interpretation results of geoelectric-resistivity measurements. Dead-end trench application significantly affected chlorophyll a, stomatal conductance, transpiration, and H2O leaf content. Dead-end trench application has no significant effect on the content of chlorophyll b, total chlorophyll content, nitrate reductase activity, stomatal density, CO2 leaf content, and photosynthesis rate.
The optimization of the Ambonese arrack fermentation using co-culture Pichia polymorpha and Kloeckera javanica
Authors: Mahulette Ferymon , Astuti Dea Indriani
Published: August 2020

Abstract. Mahulette F, Astuti DI. 2020. The optimization of the Ambonese arrack fermentation using co-culture Pichia Polymorpha and Kloeckera javanica. Biodiversitas 21: xxxx. The fermentation process of the Ambonese arrack is still uncontrollable. As various microbes are involved in the fermentation, which causes inconsistent production of ethanol content. This research aimed to optimize the production of the Ambonese arrack using Pichia polymorpha and Kloeckera javanica in inoculum ratios of 1:1, 1:2, and 1:3. The number of yeast cells and chemical characteristics was observed every three hrs up to 24 hrs. The ethanol content of the fermented coconut sap and the arrack was measured using the titration and Gas Chromatography-Mass Spectrophotometry (GC-MS) methods, respectively. The numbers of P. polymorpha and K. javanica cells at 15 hrs of fermentation were 9.9 log CFU/mL and 10.7 log CFU/mL, respectively. The reducing sugar content decreased from 525 mg/L to 296 mg/L, while the pH from 6.46 to 4.82. The highest ethanol production rate was 1.4 mg/L.3hrs. (ratio 1:1), observed at 12 hrs of the fermentation, while the highest ethanol contents in the fermented coconut sap and the arracks were 11.3 mg/L and 300.400 mg/L, respectively. Different inoculum ratios affect the sensory characteristics of Ambonese arrack. The highest average value of sensory testing was 4.6 (ratio 1:2). Thus, the inoculum ratios of P. polymorpha and K. javanica 1:2 and 1:3 has the best quality in controlled Ambonese arrack processing.
Influence of siam weed compost on soybean varieties in an agroforestry system with kayu putih (Melaleuca cajuputi)
Authors: Suryanto Priyono , Faridah Eny , Nurjanto Handojo Hadi , SUPRIYANTA SUPRIYANTA , Kastono Dody , Putra Eka Tarwaca Susila , Handayani Suci , Dewi Anggraeni Kusuma , Alam Taufan
Published: August 2020

Abstract. Suryanto P, Faridah E, Nurjanto HH, Supriyanta, Kastono D, Putra ETS, Handayani S, Dewi AK, Alam T. 2020. Influence of siam weed compost on soybean varieties in an agroforestry system with kayu putih (Melaleuca cajuputi). Biodiversitas 21: 3062-3069. Siam weed (Chromolaena odorata (L.) R.M.King & H.Rob.) has grown wild in many kayu putih (Melaleuca cajuputi Powell) forest can be utilized as compost for complementary of inorganic fertilizers in annual crops. The experiment was conducted during November-February 2020 in Menggoran Forest Resort, Playen Forest Section, Yogyakarta Forest Management District, Indonesia. The experiment was arranged in a randomized complete block design (RCBD) with three replications. The first factor was soybean varieties consisted of Anjasmoro, Dering I, and Grobogan. The second factor was siam weed compost (SWC) application consisted of 0, 5, 10, and 15 tons ha-1. The data were analyzed using Two-way ANOVA, ANCOVA, and stepwise regression. The SWC application of 10 tons ha-1 showed the highest yield of Anjasmoro, Dering I, and Grobogan were 1.42, 1.56, and 1.51 tons ha-1, respectively, or increased by118.46%, 102.60%, and 112.68%, respectively, compared to the without SWC application. The optimum dosage of SWC application for Anjasmoro, Dering I, and Grobogan were 13.05, 14.35, and 14.93 tons ha-1, respectively, with a maximum yield of 1.45, 1.59, and 1.52 tons ha-1, respectively. Soil quality and physiological parameters that had a significant influenced on the production of soybean varieties in agroforestry systems with M. cajuputi were SOM, K, LPR, TC, and PRO.
Benthic foraminifera as ecological indicators in the Tunda Island Waters, Serang District, Banten Province, Indonesia
Authors: NURRUHWATI ISNI , ARDIANSYAH FACHRI , YUNIARTI  , YULIADI LINTANG PERMATA SARI , Partasasmita Ruhyat
Published: August 2020

Abstract. Nurruhwati I, Ardiansyah F, Yuniarti, Yuliadi LPS, Partasasmita R. 2020. Benthic foraminifera as ecological indicators in the Tunda Island Waters Serang District, Banten Province, Indonesia. Biodiversitas 21: 3142-3148. Coral reef communities all across Southeast Asia are experiencing intense pressures from extensive tourism and massive coastal development. A prediction regarding coral reefs’ capacity to recover is a necessity since it is constantly exposed to damaging events such as mass bleaching and increased erosion. Tunda Island (which is highly rich in biodiversity and include coral reefs, mangrove forests, seagrass, and algae meadows) was sampled at 10 different sites in August 2019. Coral reefs are highly influenced by ecological factors such as temperature variability, salinity, DO, and pH. The assessments were conducted using the FORAM Index (FI) to accurately predict the capacity of coral reefs to recover based on relative abundances of symbiont-bearing larger benthic foraminifera in reef sediments. The FI values ranged from 3.7 to 7.9 with a median of 4.7 and the average of 5 indicates that the water quality should support the recovery of reefs by reef-building corals and symbiont-bearing larger benthic foraminifers. The lowest FI value sampled was recorded from a site with extensive human activities (FI=3.4), while the highest one was recorded from a site with minimal to none human activities (FI=7.9), which indicates that the deterioration of water quality is caused by wastes from tourism activities near the coastal area.
The diversity of pteridophytes in Siberut National Park, Mentawai Islands, West Sumatra, Indonesia
Authors: MILDAWATI MILDAWATI , SOBIR SOBIR , SULISTIJORINI SULISTIJORINI , Chikmawati Tatik
Published: August 2020

Abstract. Mildawati, Sobir, Sulistijorini, Chikmawati T. 2020. The diversity of pteridophytes in Siberut National Park, Mentawai Islands, West Sumatra, Indonesia. Biodiversitas 21: 3200-3208. The diversity of Pteridophytes in the Siberut National Park (Siberut NP) Mentawai Islands, West Sumatra, is still very well preserved, but the information is limited. This research aimed to study the divesrity of Pteridophyte species in the Siberut NP in order to provide its taxonomic information using morphological data. The study consisted of exploration to collect plant specimens directly in the field, observation and identification of herbarium specimens in Herbarium Bogoriense and verification using online websites from various sources. A total of 69 species of Pteridophytes classified into 36 genera, and 20 families were recorded in the Siberut NP with Polypodiaceae has the most species diversity with 12 species of 6 genera. As many as 14 species are new records for Siberut Island, i.e. Selaginella involvens (Sw.) Spring (Selaginellaceae), Asplenium simile Blume (Aspleniaceae), Diplazium montanum V.A.V.R (Athyriaceae), Stenochlaena palustris (Burm) Bedd. (Gleichnaceae), Davallia solida (Forst.) Sw. (Davalliaceae), Callistopteris apiifolia (C.Presl) Copel., Crepidomanes humile Bosch (Hymenophyllaceae), Lindsaea carvifolia Kramer, Lindsaea doryphora Kramer, Lindsaea napaea Aldrew. (Lindsaeaceae), Nephrolepis biserrata (Sw.) Schott (Nephrolepidaceae), Ceratopteris thalictroides (L.) Brongn., Haplopteris ensiformis (Sw.) E.H.Crane and Syngramma alismifolia (Pr.) J.Sm. (Pteridaceae). The habitats of Pteridophytes found were mostly terrestrial and epiphytic (34 species each), while aquatic habitat was only found to one species. The results of this study will serve as baseline information of the plant diversity in the Siberut NP that can be used as the reference for future studies on Pteridophytes and to develop their conservation strategy in the region.
Diversity and distribution bromeliads plants as breeding habitat for mosquito larvae (Diptera: Culicidae) in Bogor, Indonesia
Authors: Ikhsan M , HADI UPIK KESUMAWATI , SOVIANA SUSI
Published: August 2020

Abstract. Ikhsan M, Hadi UK, Soviana S. 2020. Diversity and distribution bromeliads plants as breeding habitat for mosquito larvae (Diptera: Culicidae) in Bogor, Indonesia. Biodiversitas 21: 3494-3498. Bromeliads leaf structure can hold water and has potential as the breeding ground for mosquitoes. Information regarding the potential of bromeliads as a habitat for mosquito larvae in Indonesia is limited. This study aims to identify the diversity of bromeliads and their potential as breeding habitat for mosquito larvae in West Bogor Sub-district, Bogor City, West Java, Indonesia. Determination of the sample using purposive sampling method. Larvae collections were carried out on bromeliads that were outside the building in December 2019 to Maret 2020. The collection used a 50 ml syringe that was modified using a small pipe. Total bromeliads collected were 117 consisted of Neoregelia, Cryptanthus, dan Alcantarea from 50 observation points. The percentage of mosquito larvae presence from bromeliads was 74.4%. Total larvae collected were 859 larvae consisting of Aedes albopictus, Aedes aegypti, Aedes spp., Armigeres subalbatus, and Culex quinquefasciatus. The diversity of mosquito larvae found in the bromeliads were low (H'=0.16), while the dominant species of mosquito larvae were Ae. albopictus (61.55%) and confirmed as the second vector of Dengue Fever in Indonesia. The results of this study indicate that bromeliads have potential as a breeding site for mosquito larvae in West Bogor Sub-district, Bogor City.
Short Communication: Molecular characteristics and phylogenetic relationships of silurid catfishes (Kryptopterus, Ompok and Phalacronotus) from the Kampar River, Indonesia, based on the cytochrome b gene
Authors: Elvyra Roza , SOLIHIN DEDY DURYADI , AFFANDI RIDWAN , JUNIOR MUHAMMAD ZAIRIN , SUHENDRA MEYLA
Published: August 2020

Abstract. Elvyra R, Solihin DD, Affandi R, Junior MZ, Suhendra M. 2020. Short Communication: Molecular characteristics and phylogenetic relationships of silurid catfishes (Kryptopterus, Ompok, and Phalacronotus) from the Kampar River, Indonesia, based on the cytochrome b gene. Biodiversitas 21: 3539-3546. The study of molecular characteristics and phylogenetic relationships among silurid catfishes (Kryptopterus, Ompok, and Phalacronotus) is very scarce. Existing data are mostly based on morphological characters. Genetic markers among Kryptopterus, Ompok, and Phalacronotus can be analyzed by exploring the nucleotide and amino acid sequences of the mitochondrial cytochrome b gene region (906 base pairs). This study aims to identify molecular characteristics and phylogenetic relationships of silurid catfishes from the Kampar River based on the cytochrome b gene. This research examined 14 silurids of four species from three genera in the Kampar River of Indonesia. Molecular phylogenetic trees were constructed using the neighbor-joining method. This study revealed that three amino acid sites can be used as specific genetic markers for characterizing Kryptopterus limpok. They are E (glutamic acid) at site 123, V (valine) at site 155, and M (methionine) at site 156. Two amino acid sites can be used as specific genetic markers of Ompok spp., A (alanine) at site 201 for O. hypophthalmus, and S (serine) at site 282 for O. eugeneiatus. Two amino acid sites, I (isoleucine) at site 197 and L (leucine) at site 284, can be used to identify Phalacronotus apogon from the Kampar River, Indonesia. The phylogram results based on genetic distance (p-distance) from nucleotide cytochrome b sequences generally showed that intraspecies of K. limpok, O. eugeneiatus, O. hypophthalmus and P. apogon from the Kampar River formed relationship groups within each species supported by high bootstrap values. Additionally, based on genetic distance, K. limpok data from the Kampar River samples and GenBank formed one relationship group within species of K. limpok with a high bootstrap value. This study proves that cytochrome b gene can be used to identify molecular characteristics and phylogenetic relationships of K. limpok, O. eugeneiatus, O. hypophthalmus, and P. apogon from the Kampar River, Indonesia.
Exploration, phenolic content determination, and antioxidant activity of dominant pteridophytes in Gunung Malang Village, Mount Halimun Salak National Park, Indonesia
Authors: RINDITA RINDITA , Anggia Vivi , RAHMAESA EKA , DEVI RETNA KUSUMA , ALAWIYAH LIDIA FATMAH
Published: August 2020

Abstract. Rindita, Anggia V, Rahmaesa E, Devi RK, Alawiyah LF. 2020. Exploration, phenolic content determination, and antioxidant activity of dominant pteridophytes in Gunung Malang Village, Mount Halimun Salak National Park, Indonesia. Biodiversitas 21: 3676-3682. Pteridophytes have been known to have pharmacological activities as an antioxidant, anti-inflammation, antimicrobials, and anticancer, such as Selaginella sp. In this research, exploration was conducted in Gunung Malang Village, Mount Halimun Salak National Park (TNGHS), West Java. Samples were collected by purposive sampling method, related to its abundant populations, Histiopteris incisa, Nephrolepis biserrata, and Selaginella willdenowii were collected based on different height and light intensity. Abiotic factors were measured to know their ecological requirements. Samples were extracted using ultrasonic method. Organoleptic test, yield, and phytochemical screening were done for the crudes extract. Total phenolic level was determined with Folin-Ciocalteu method and antioxidant activity test with DPPH. Phytochemical screening showed that H. incisa contains phenolics, saponins, tannins, and steroids; N. biserrata contains phenolics, flavonoids, and saponins while S. willdenowii contains phenolics, alkaloids, flavonoids, saponins, and tannins. Phenolic content of H. incisa and N. biserrata from 700 m. asl.was 9.8523 mg GAE/g ± 0.9694 and 17.5399 mg GAE/g ± 0.5350 respectively, from 1000 m. asl. was 18.1231 mgGAE/g ±2.1535 and 8.8468 mg GAE/g ± 0.1437. S. willdenowii extract collected from shaded canopy forest was 19.2324 mg GAE/g ± 0.6041 and opened canopy forest was 38.7087 mg GAE/g ± 1.484. Antioxidant IC50 of H. incisa and N. biserrata from 700 m. asl. respectively was 96.4271 ppm and 85.1907 ppm, while from 1000 m. asl. was 75.6381 ppm and 95.0678 ppm, S. willdenowii from the closed canopy is 101.7326 ppm, while from the opened canopy was 92.0998 ppm. This study shows that all ferns tested gave significant antioxidant activity and was influenced by environmental factors.
Artificial Intelligence in plastic surgery: What is it? Where are we now? What is on the horizon?
Authors: Murphy DC , Saleh DB
Published: August 2020

Introduction

An increasing quantity of data is required to guide precision medicine and advance future healthcare practices, but current analytical methods often become overwhelmed. Artificial intelligence (AI) provides a promising solution. Plastic surgery is an innovative surgical specialty expected to implement AI into current and future practices. It is important for all plastic surgeons to understand how AI may affect current and future practice, and to recognise its potential limitations.

Methods

Peer-reviewed published literature and online content were comprehensively reviewed. We report current applications of AI in plastic surgery and possible future applications based on published literature and continuing scientific studies, and detail its potential limitations and ethical considerations.

Findings

Current machine learning models using convolutional neural networks can evaluate breast mammography and differentiate benign and malignant tumours as accurately as specialist doctors, and motion sensor surgical instruments can collate real-time data to advise intraoperative technical adjustments. Centralised big data portals are expected to collate large datasets to accelerate understanding of disease pathogeneses and best practices. Information obtained using computer vision could guide intraoperative surgical decisions in unprecedented detail and semi-autonomous surgical systems guided by AI algorithms may enable improved surgical outcomes in low- and middle-income countries. Surgeons must collaborate with computer scientists to ensure that AI algorithms inform clinically relevant health objectives and are interpretable. Ethical concerns such as systematic biases causing non-representative conclusions for under-represented patient groups, patient confidentiality and the limitations of AI based on the quality of data input suggests that AI will accompany the plastic surgeon, rather than replace them.
Universal Credit, lone mothers and poverty: some context and challenges for social work with children and families
Authors: Carey Malcolm , Bell Sophie
Published: August 2020

Universal Credit is a streamlined benefits delivery system initially introduced in the UK in 2008. Conditionality-based welfare policies are increasingly international in scale, and are now widely adopted by neoliberal governments on the basis that paid employment offers the most efficacious route out of poverty for citizen-subjects. Numerous studies suggest otherwise and highlight their negative impact upon the social rights, lived experiences and attempts to alleviate poverty for claimants. This article analyses the effect of the reformed benefit system and wider workfare policies upon lone mothers, including as a consequence of engagement with an ever-more stigmatising benefit system, and associated risks posed by sanctions or precarious low-paid employment. It highlights some of the consequences for social work with children and families of Universal Credit, including ongoing tensions and challenges created for the profession by the punitive policies of the workfare-oriented centaur state.
Prospective detection of cervical dysplasia with scanning angle-resolved low coherence interferometry
Authors: Kendall Wesley Y. , Ho Derek , Chu Kengyeh , Zinaman Michael , Wieland Daryl , Moragne Kandis , Wax Adam
Published: August 2020

We present a prospective clinical study using angle-resolved low-coherence interferometry (a/LCI) to detect cervical dysplasia via depth resolved nuclear morphology measurements. The study, performed at the Jacobi Medical Center, compares 80 a/LCI optical biopsies taken from 20 women with histopathological tissue diagnosis of co-registered physical biopsies. A novel instrument was used for this study that enables 2D scanning across the cervix without repositioning the probe. The main study goal was to compare performance with a previous clinical a/LCI point-probe instrument [Int. J. Cancer 140, 1447 (2017)10.1002/ijc.30539] and use the same diagnostic criteria as in that study. Tissue was classified in two schemes: non-dysplastic vs. dysplastic and low-risk vs. high-risk, with the latter classification aligned with clinically actionable diagnosis. High sensitivity (non-dysplastic vs. dysplastic: 0.903, low-risk vs. high-risk: 1.000) and NPV (0.930 and 1.000 respectively) were obtained when using the previously established decision boundaries, showing the success of the scanning a/LCI instrument and reinforcing the clinical viability of a/LCI in disease detection.