bluuebunny/crossref_metadata_2025 huggingface dataset:
Seasonal spatial-temporal trends of vegetation recovery in burned areas across Africa
Authors: Maillard Oswaldo , Ribeiro Natasha , Armstrong Amanda , Ribeiro-Barros Ana I. , Andrew Samora Macrice , Amissah Lucy , Shirvani Zeinab , Muledi Jonathan , Abdi Omid , Azurduy Huascar , Silva João M. N. , Syampungani Stephen , Shamaoma Hastings , Buramuge Victorino
Published: February 2025

Africa is entering a new fire paradigm, with climate change and increasing anthropogenic pressure shifting the patterns of frequency and severity. Thus, it is crucial to use available information and technologies to understand vegetation dynamics during the post-fire recovery processes. The main objective of this study was to evaluate the seasonal spatio-temporal trends of vegetation recovery in response to fires across Africa, from 2001 to 2020. Non-parametric tests were used to analyze MODIS Normalized Difference Vegetation Index (NDVI) products comparing the following three-month seasonal periods: December-February (DJF), March-May (MAM), June-August (JJA), and September-November (SON). We evaluated the seasonal spatial trends of NDVI in burned areas by hemisphere, territory, or country, and by land cover types, and fire recurrences, with a focus on forested areas. The relationships between the seasonal spatial trend and three climatic variables (i.e. maximum air temperature, precipitation, and vapor pressure deficit) were then analyzed. For the 8.7 million km2 burned in Africa over the past 22 years, we observed several seasonal spatial trends of NDVI. The highest proportions of areas with increasing trend (p < 0.05) was recorded in MAM for both hemispheres, with 22.0% in the Northern Hemisphere and 17.4% in the Southern Hemisphere. In contrast, areas with decreasing trends (p < 0.05), showed 4.8–5.5% of burned area in the Northern Hemisphere, peaking in JJA, while the Southern Hemisphere showed a range of 7.1 to 10.9% with the highest proportion also in JJA. Regarding land cover types, 48.0% of fires occurred in forests, 24.1% in shrublands, 16.6% in agricultural fields, and 8.9% in grasslands/savannas. Consistent with the overall trend, the area exhibiting an increasing trend in NDVI values (p < 0.05) within forested regions had the highest proportion in MAM, with 19.9% in the Northern Hemisphere and 20.6% in the Southern Hemisphere. Conversely, the largest decreasing trend (p < 0.05) was observed in DJF in the Northern Hemisphere (2.7–2.9%) and in JJA in the Southern Hemisphere (7.2–10.4%). Seasonally, we found a high variability of regeneration trends of forested areas based on fire recurrences. In addition, we found that of the three climatic variables, increasing vapor pressure deficit values were more related to decreasing NDVI levels. These results indicate a strong component of seasonality with respect to fires, trends of vegetation increase or decrease in the different vegetation covers of the African continent, and they contribute to the understanding of climatic conditions that contribute to vegetation recovery. This information is helpful for researchers and decision makers to act on specific sites during restoration processes.
Study on disaster-causing probability evaluation of gas pipeline in karst area
Authors: Li Qiaochu , Zhang Peng
Published: February 2025

In this paper, a disaster-causing probability evaluation method of gas pipelines in karst areas based on disaster system theory and vulnerability theory is proposed. The hazard evaluation index system of gas pipeline disaster events in karst areas is established from three aspects: the activity of disaster-prone environment, the risk of disaster factors and the vulnerability of disaster-bearing bodies. Combined with the advantages of information transmission and updating of the Bayesian network model, the hazard probability of disaster events is gradually calculated from a multi-level perspective. Based on the structural reliability theory, the vulnerability evaluation function of gas pipelines in karst areas is established by considering the interaction between disaster hazard intensity and disaster resistance ability. Meanwhile, the design checkpoint method and finite element simulation are used to calculate the vulnerability probability. Finally, a new disaster-causing probability evaluation approach for gas pipelines in karst areas is formed, which comprehensively considers the event hazard and the pipeline vulnerability. The work presented in this paper can provide a reference for the safety management and accident prevention of gas pipelines crossing the karst areas.
Women’s enrollment in community-based health insurance and its determinants in Sidama national regional state, Ethiopia, 2024: A multilevel analysis
Authors: Debessa Kare Chawicha , Negeri Keneni Gutema , Dangisso Mesay Hailu
Published: February 2025

Introduction

Accessing affordable and high-quality healthcare remains a persistent challenge in low- and middle-income countries like Ethiopia. Community-based health insurance (CBHI) programs offer a promising solution to expand healthcare coverage and provide financial protection, particularly for vulnerable populations such as women. This study aimed to investigate the factors that influence CBHI enrollment among women in Sidama National Regional State, Ethiopia, using a multilevel analysis.

Methods

A community-based cross-sectional study was conducted using a multistage sampling technique from December 15th to January 12th, 2024, in the central Sidama zone, Sidama National Regional State. The study included 835 women aged 18 years and older, residing both in rural and urban areas. Face-to-face interviews were conducted using a pre-tested questionnaire to collect comprehensive data on socio-demographic, economic, and scheme-related factors. Data collection utilized the Open Data Kit mobile application, and data analysis was performed using Stata version 16, employing multilevel modified Poisson modeling to identify determinants of CBHI enrollment.

Results

Among 845 samples, 835 women were interviewed, resulting in a response rate of 98.8%. Individual-level factors such as older age (APR = 1.02, 95%CI: 1.01–1.03, p<0.001), larger family size (APR = 1.09, 95%CI: 1.03–1.16, p = 0.001), and moderate wealth index (APR = 2.72, 95%CI: 1.28–5.79, p = 0.009) showed positive associations with CBHI enrollment, depicted a higher likelihood of individuals joining the insurance scheme. In addition, at the community level, higher rates of women’s literacy (APR = 1.73, 1.18–2.55, p = 0.005), and women’s autonomy (APR = 2.64, 95%CI: 1.50–4.65, p = 0.001) were positively correlated with CBHI enrollment.

Conclusions

The study revealed that the enrollment rate among women in the CBHI scheme was 35%, indicating a need for improved outreach efforts. Significant factors that affected enrollment included older age, larger family size, and moderate wealth. Additionally, positive community-level influences such as higher literacy rates and increased autonomy for women contributed to higher enrollment. To improve CBHI enrollment, the target should focus on younger women and those from smaller families. Financial support, such as subsidies for low-income women, can also encourage participation. Investing in women’s literacy and empowerment programs will enable them to make informed health choices, thereby increasing enrollment. Finally, ongoing research is necessary to track enrollment trends and identify barriers. Utilizing qualitative methods will yield insights into women’s perceptions of CBHI, facilitating more effective strategies. Implementing these recommendations can enhance women’s access to healthcare through CBHI.
Higher risk of carotid plaque among lean individuals with non-alcoholic fatty liver disease: A retrospective study
Authors: Xue Jiangfeng , Zhao Lun , Shao Liang , Zhang Huiwang , Feng Yewei , Shuai Ping
Published: February 2025

Background

Lean individual with non-alcoholic fatty liver disease (L-NAFLD) is a prominent area of research, yet its pathogenesis and association with other diseases such as atherosclerotic cardiovascular disease remain uncertain.

Object

A retrospective study, investigate the association between non-alcoholic fatty liver disease (NAFLD) and carotid plaque (CP) in lean [body mass index (BMI) <24Kg/m2] and non-lean (BMI≥24Kg/m2) populations, as well as identify the related influence factors.

Method

3,587 participants were eligible and categorized into 4 groups based on the presence with CP and BMI, binary logistic regression analysis was utilized alongside other statistical methods.

Results

L-NAFLD participants had a 1.395-fold higher risk of CP compared to lean individuals without NAFLD. Age, gender, systolic blood pressure, low-density lipoprotein cholesterol, fasting blood glucose, and Fibrosis-4 index (FIB-4) were identified as independent risk factors with cutoff values lower than the normal upper limits. However, this association was not observed among non-lean participants, regardless of confounding factors adjustment. Moreover, the impact of FIB-4 on the association of NAFLD and CP was more significant in lean CP participants (OR = 1.360 for 1.30 ~ 2.67, and OR = 2.002 for >2.67~<3.48) than in non-lean CP ones.

Conclusion

The L-NAFLD population had a higher risk of CP, while lean CP individuals experienced more severe liver fibrosis. Implementing stricter management of risk factors may improve the health status of high-risk populations.
Unsupervised monocular depth estimation with omnidirectional camera for 3D reconstruction of grape berries in the wild
Authors: Tamura Yasuto , Utsumi Yuzuko , Miwa Yuka , Iwamura Masakazu , Kise Koichi
Published: February 2025

Japanese table grapes are quite expensive because their production is highly labor-intensive. In particular, grape berry pruning is a labor-intensive task performed to produce grapes with desirable characteristics. Because it is considered difficult to master, it is desirable to assist new entrants by using information technology to show the recommended berries to cut. In this research, we aim to build a system that identifies which grape berries should be removed during the pruning process. To realize this, the 3D positions of individual grape berries need to be estimated. Our environmental restriction is that bunches hang from trellises at a height of about 1.6 meters in the grape orchards outside. It is hard to use depth sensors in such circumstances, and using an omnidirectional camera with a wide field of view is desired for the convenience of shooting videos. Obtaining 3D information of grape berries from videos is challenging because they have textureless surfaces, highly symmetric shapes, and crowded arrangements. For these reasons, it is hard to use conventional 3D reconstruction methods, which rely on matching local unique features. To satisfy the practical constraints of this task, we extend a deep learning-based unsupervised monocular depth estimation method to an omnidirectional camera and propose using it. Our experiments demonstrate the effectiveness of the proposed method for estimating the 3D positions of grape berries in the wild.
Impact of health education on knowledge retention among caregivers of hypertensive patients: A prospective cross-sectional study in rural Malawi
Authors: Maluwa Chikondi , Kapira Sitalire , Chuljerm Hataichanok , Parklak Wason , Kulprachakarn Kanokwan
Published: February 2025

Hypertension is a widespread and life-threatening condition affecting one-third of adults globally. In low- and middle-income countries, like Malawi, the burden of hypertension is escalating due to inadequate healthcare resources and lifestyle changes. Family members often become primary caregivers, playing a crucial role in managing hypertension through support and adherence to treatment. This study examined caregivers’ knowledge retention by evaluating their pre- and post-health education knowledge levels. This was a prospective cross-sectional study in Neno, Malawi, a rural setting. 422 caregivers were enrolled from the Integrated Chronic Care Clinic (IC3). A structured questionnaire was used to collect baseline, post-health education, and week six data. Using SPSS V 22.0, comparison of knowledge, attitude, and practices (KAP) scores, correlation between KAP and between KAP and social demographic characteristics were done using Wilcoxon signed-rank test, Pearson correlation, and independent t-test respectively. Among the 422 caregivers who participated in the study, 267 (63.2%) were females and mean age was 44.94 years. The baseline mean knowledge level score was 9.5 (38.0%) and rose to 21.08 (84.3%) p = 0.000 immediate post-health education and a 2.1% decrease 20.54 (82.2%) p<0.001 at week six from the immediate post health education score. Attitude improved from 16.76 (93.1%) at baseline to 17.74 (98.6%) at the six-week mark. Similarly, the mean practice score rose from 25.24 (78.9%) at baseline to 27.42 (85.7%) at week six. There was a positive correlation between KAP while age had a negative correlation with knowledge (r = -0.146; p = 0.003). There was a significant difference between different education levels on knowledge retention p = 0.009. There was a positive and good knowledge retention among caregivers of hypertensive patients after health education at the week six mark. With improved knowledge and the ability to retain it resulting in improved attitude and practices, caregivers are a cornerstone for continued and improved hypertension care for the patients.
Impact of pharmacist-led intervention for reducing drug-related problems and improving quality of life among chronic kidney disease patients: A randomized controlled trial
Authors: Zafar Roheena , Rehman Inayat Ur , Shah Yasar , Ming Long Chiau , Goh Khang Wen , Suleiman Amal K , Khan Tahir Mehmood
Published: February 2025

Introduction

Chronic kidney disease (CKD) patients suffer from different comorbid conditions and are prone toward drug-related problems (DRPs) which affect their clinical parameters as well as quality of life (QoL). This study was aimed to evaluate the impact of clinical pharmacist-led interventions on the mean number of DRPs and the mean QoL score difference per patient DRPs in CKD patients.

Method

An open-labeled, randomized control trial performed from April 2023 to July 2023 in the nephrology unit of a tertiary care setting in Peshawar Pakistan. Those patients who met the inclusion criteria were randomized into two groups 1:1, i.e., control and intervention group. Clinical pharmacists identified the DRPs at baseline using Pharmaceutical Care Network Europe (PCNE) 9.1 guidelines. The QoL of patients were assessed at baseline and endpoint by using the Functional Assessment of Non-Life-Threatening Conditions (FANLTC) questionnaire.

Results

A total of 100 patients were recruited having 50 in each group. The pharmacist identified a total of n = 230 DRPs in the intervention group, majority of the DRPs were attributed to inappropriate drug selection according to guidelines/formulary”; “inappropriate combinations of drugs or with herbal medications or dietary supplements”; and situations where “too many different drugs or active ingredients were prescribed”. There was 46.52% reduction in the DRPs while comparing baseline and endpoint interventions suggested by pharmacist in the intervention group. The clinical pharmacist provided interventions in order to resolve the DRPs, and 37.40% interventions were accepted and fully implemented; 31.30% of the interventions were accepted and partially implemented. The clinical pharmacist identification and proposed intervention for DRPs contributed to a statistically significant improvement in QoL, from mean ±  SD scored 58.64 ±  9.10 at the baseline to 74.48 ± 10.11 at the endpoint, with a p-value of < 0.001.

Conclusion

A significant improvement in the QoL and laboratory parameters for patients with CKD following clinical pharmacist-led interventions having proposed interventions were implemented successfully from baseline to endpoint; however, a considerable number of proposed interventions were not accepted and implemented.
Association of adenylate cyclase activity in vasopressor-type neurally mediated syncope based on the α2b-AR gene
Authors: Komiyama Tomoyoshi , Ayabe Kengo , Orita Kunzo , Sasaki Ayumi , Kitamura Yuka , Matsuzawa Hideyuki , Yamashita Teppei , Nagata Eiichiro , Kanatani Yasuhiro , Yoshioka Koichiro
Published: February 2025

Neurally mediated syncope (NMS) arises from a neural reflex; however, its underlying cause remains unknown. Previous research has shown that variations in the Gi-α signal transduction rate led to changes in adenylate cyclase (AC) activity levels. Thus, we hypothesized that these fluctuations in AC activity could contribute to NMS. This study aimed to investigate the receptor genes associated with glutamate (Glu) repeat polymorphism sites Glu12 and Glu9 in the α2B-AR gene. A total of 50 patients with vasodepressor-type (VT)-NMS and 20 healthy volunteers were included in this study. We assessed AC activity levels and blood pressure responses during the head-up tilt (HUT) test and conducted a Glu repeat polymorphism analysis to explore its potential association with NMS. Our findings showed significantly higher AC activity in patients with the Glu12/12 homotype than healthy volunteers across all four HUT test points. Conversely, patients with the Glu9/12 heterotype exhibited a significant difference only 10 min after the test initiation, suggesting a pronounced activation effect of the β2-AR Gs-α subunit in these individuals. Both genotypes displayed the greatest blood pressure fluctuations under 70° tilt stress, with patients with Glu12/12 showing consistent cardiac load and higher values than those with Glu9/12 at two points. Notably, the frequency of NMS onset within 20 min during the tilt test varied, with one Glu12/12 patient and seven Glu9/12 patients experiencing syncope. Additionally, patients with the Glu9/12 heterotype were found to have a higher risk of syncope after prolonged standing compared to those with the Glu12/12 homotype. These results suggest that patients with the Glu12/12 homotype exhibit elevated AC activity levels, which may help increase blood pressure to prevent syncope. This study underscores that variations in AC activity among different gene types could influence the frequency of NMS onset during standing.
Characteristics of chicken production systems in rural Burkina Faso: A focus on One Health related practices and food security
Authors: Dione Michel , Ilboudo Guy , Ouedraogo Abdoul Aziz , Ima Sidonie Aristide , Ouedraogo Brice , Knight-Jones Theodore , Kagambèga Assèta , Alders Robyn
Published: February 2025

In addition to having cultural importance, village chicken production remains an important source of cash income for most rural households in Burkina Faso. However, strict biosecurity and good management of chicken flocks are required to reduce the risk of exposure of communities to chicken waste at household level. We characterized village production systems in rural Burkina Faso in relation to importance to food security, biosecurity, husbandry and chicken health management. We surveyed 483 chicken-producing households and carried out 20 focus group discussions separately with men and women chicken producers in Boussouma commune, a typical rural setting. Crop farming was reported as the main income-generating activity carried out by chicken producers (79.5%). Seveny six per cent of households sold chicken to local markets. Chicken production and sales were aligned to social/cultural events (religious festivals, weddings, etc…) and school-fee payment period. While men spent more revenues from chicken production on agriculture and household equipment’s, women spent more on food, education and medical expenses. The chicken management system is mainly extensive scavenging, with most farmers (81.4%) keeping 5 to 50 birds with little or no supplementary feeding and rudimentary housing. Most producers indicated that Newcastle disease was the main cause of chicken mortality. While men consider high disease burden, lack of finance, and poor chicken housing as the major constraints, women prioritized the lack of adequate chicken housing, lack of feeds and limited access to veterinary services. With locally adapted interventions that build one a One Health approach, village chicken keeping has the potential to secure and greatly improve smallholder livelihoods and household food security, while preserving public health in Burkina Faso.
The impact of polypharmacy on health outcomes in the aged: A retrospective cohort study
Authors: Boateng Irene , Pascual Carlos Rodriguez , Grassby Paul , Asghar Zahid , Ibrahim Kinda
Published: February 2025

Objectives

To estimate the prevalence of polypharmacy among community-dwelling adults in the UK and determine its association with mortality, hospitalization, adverse drug reactions and falls at one and five years. To also determine the effect of polypharmacy on the outcomes in different patient groups.

Methods

A retrospective cohort study was carried out using 1000 patients aged 75 years and above from the Clinical Practice Research Datalink. The study periods for the one- and five-years analysis were January 2010-December 2010 and January 2010-December 2014 respectively. Sociodemographic and clinical variables were retrieved using medical and product codes. Polypharmacy was defined as the use of five or more medicines. The association between polypharmacy and mortality, falls, adverse drug reactions, or hospitalization was determined using cox regression analysis while confounding for age, sex, Charlson’s comorbidity index, potentially inappropriate medicines, hospitalization prior to study, and falls prior to study. Subgroup analysis was used to determine the effect of polypharmacy on the outcomes for different patient groups.

Key findings

977 people were reviewed. 36% were male and the mean age was 83 years. The prevalence of polypharmacy was 47%. Adjusted hazard ratios with their 95% confidence intervals for association between polypharmacy and outcomes at five years were: mortality 1.60 (1.30–2.00), hospitalization 1.49 (1.30–1.70), falls 1.49 (0.90–2.40) and adverse drug reactions 0.97 (0.50–1.80). The results for the one-year analysis were mortality 2.37 (1.40–3.90), hospitalization 2.47 (1.40–4.30), and falls 0.37 (0.03–4.00).

Conclusion

Polypharmacy was found to be a risk factor for mortality and hospitalization. The risk increased with an increase in age, potentially inappropriate medicines and comorbidities.
Shedding light on development: Leveraging the new nightlights data to measure economic progress
Authors: Jhamb Prachi , Ferreira Susana , Stephens Patrick , Sundaram Mekala , Wilson Jonathan
Published: February 2025

Nightlights (NTL) have been widely used as a proxy for economic activity, despite known limitations in accuracy and comparability, particularly with outdated Defense Meteorological Satellite Program (DMSP) data. The emergence of newer and more precise Visible Infrared Imaging Radiometer Suite (VIIRS) data offers potential, yet challenges persist due to temporal and spatial disparities between the two datasets. Addressing this, we employ a novel harmonized NTL dataset (VIIRS + DMSP), which provides the longest and most consistent database available to date. We evaluate the association between newly available harmonized NTL data and various indicators of economic activity at the subnational level across 34 countries in sub-Saharan Africa from 2004 to 2019. Specifically, we analyze the accuracy of the new NTL data in predicting socio-economic outcomes obtained from two sources: 1) nationally representative surveys, i.e., the household Wealth Index published by Demographic and Health Surveys, and 2) indicators derived from administrative records such as the gridded Human Development Index and Gross Domestic Product per capita. Our findings suggest that even after controlling for population density, the harmonized NTL remain a strong predictor of the wealth index. However, while urban areas show a notable association between harmonized NTL and the wealth index, this relationship is less pronounced in rural areas. Furthermore, we observe that NTL can also significantly explain variations in both GDP per capita and HDI at subnational levels.
A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar ‘Varnes’
Authors: Sargent Daniel James , Buti Matteo , Martens Stefan , Pugliesi Claudio , Aaby Kjersti , Røen Dag , Yadav Chandra Bhan , Fernández Fernández Felicidad , Alsheikh Muath , Davik Jahn , Price R. Jordan
Published: February 2025

Cultivated raspberries (Rubus idaeus L.) most commonly bear small, red, highly aromatic fruits. Their colour is derived predominantly from anthocyanins, water soluble polyphenolic pigments, but as well as red forms, there exist cultivars that display yellow- and apricot-coloured fruits. In this investigation, we used a multi-omics approach to elucidate the genetic basis of the apricot fruit colour in raspberry. Using metabolomics, we quantified anthocyanins in red and apricot raspberry fruits and demonstrated that, in contrast to red-fruited raspberries, fruits of the apricot cultivar ‘Varnes’ contain low concentrations of only a small number of anthocyanin compounds. By performing RNASeq, we revealed differential expression patterns in the apricot-fruited ‘Varnes’ for genes in the anthocyanin biosynthesis pathway and following whole genome sequencing using long-read Oxford Nanopore Technologies sequencing, we identified a CACTA-like transposable element (TE) in the second exon of the Anthocyanidin synthase (Ans) gene that caused a truncated predicted ANS protein. PCR confirmed the presence in heterozygous form of the transposon in an unrelated, red-fruited cultivar ‘Veten’, indicating apricot fruit colour is recessive to red and that it may be widespread in raspberry germplasm, potentially explaining why apricot forms appear at regular intervals in modern raspberry breeding populations.
Streptococcus mutans regulates ubiquitin modification of Candida albicans in the bacterial-fungal interaction
Authors: Zhang Yixin , Gu Zhen , Li Zhengyi , Wu Qinrui , Xu Xin , Peng Xian
Published: February 2025

The ecological interplay between Streptococcus mutans and Candida albicans within dental plaque biofilms is an important factor driving pathogenesis of dental caries. This study aimed to investigate S. mutans regulation of C. albicans growth and virulence through extracellular membrane vesicles (EMVs) and modulation of ubiquitination, a key protein post-translational modification. We established a transwell co-culture model to enable “contact-independent” interactions between S. mutans and C. albicans. S. mutans EMVs were found to directly associate with C. albicans cells and promote biofilm formation and growth. Quantitative ubiquitination profiling revealed S. mutans dramatically alters the ubiquitination landscape in C. albicans. We identified 10,661 ubiquitination sites across the C. albicans proteome and their enrichment in pathways related to translation, metabolism, and stress adaptation. Co-culture with S. mutans led to upregulation of ubiquitination on 398 proteins involved in sugar catabolism and generation of reducing power. S. mutans upregulated ubiquitination of superoxide dismutase-3 of C. albicans, inducing its degradation and heightened reactive oxygen species levels, and concomitantly stimulated C. albicans growth. Our findings elucidate EMVs and ubiquitination modulation as key mechanisms governing the S. mutans-C. albicans interplay and provide new insights into the promotion of a cariogenic oral biofilm environment. This study significantly advances understanding of the complex molecular interactions underlying dental plaque dysbiosis and caries pathogenesis.
Marek’s disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius
Authors: Bao Chenyi , Chu Jun , Gao Qi , Yang Shasha , Gao Xiaoyu , Chen Wenwen , Yang Fuchun , Jiang Fei , Tong Chenxi , Lei Mingyi , Jiao Linlin , Li Jitong , Wei Kexin , Lian Xue , Li Kai , Tikoo Suresh Kumar , Osterrieder Nikolaus , Babiuk Lorne A. , Li Yufeng , Jung Yong-Sam , Qian Yingjuan
Published: February 2025

Marek’s disease virus (MDV), an alphaherpesvirus, causes severe immunosuppression and T cell lymphomas in chickens, known as Marek’s disease (MD), an economically important poultry disease primarily controlled by vaccination. Importantly, it also serves as a comparative model for studying herpesvirus-induced tumor formation in humans. MDV encodes more than 100 genes, most of which have unknown functions. MDV LORF1 is unique to serotype I MDV (MDV-1), lacking homologs in other herpesviruses, and has not been explored yet. To this end, an infectious bacterial artificial chromosome (BAC) harboring the complete genome of the MDV-1 very virulent strain Md5 was generated, and the rescued rMd5 maintained biological properties similar to the parental virus both in vitro and in vivo. Subsequently, rMd5ΔLORF1, a recombinant Md5 virus deficient in pLORF1 expression, was generated by a frameshift mutation in the LORF1 gene. Chickens infected with rMd5ΔLORF1 exhibited a lower mortality rate and delayed bursal atrophy than those infected with the parental rMd5 and the revertant virus (rMd5-reLORF1). Consistently, viral loads of rMd5ΔLORF1 were obviously lower than those of rMd5 or rMd5-reLORF1 in the bursa, but not in the spleen. Importantly, we found that pLORF1 deficiency impairs viral replication in bursal B cells. Furthermore, we showed that pLORF1 associated with the cellular membrane, interacted with MDV structural proteins, and exhibited punctate colocalization with tegument or capsid proteins in the cytoplasm. Taken together, this study demonstrates for the first time that the MDV-1 unique gene LORF1 is involved in MDV-induced bursal atrophy but not in tumor formation.
Us and them: We draw together: Exploring inclusivity in a community mural project
Authors: Hall Kimberly Ellen , El Masri Omar
Published: February 2025

The Community Mural Project in St Pauls, Cheltenham, United Kingdom reflects on the dynamics of ‘us and them’ within a community mural initiative. It seeks to develop inclusive social spaces through participatory arts-based research using drawing. The article also addresses the complexities of power and influence in mural arts. Furthermore, it examines the interconnectedness of community and the role of drawing and illustration methods in understanding and addressing inclusivity and exclusivity. The project takes place in St Pauls, a mixed-class area of Cheltenham known for its annual festivals, where observations and reflections from the interdisciplinary team contribute to a comprehensive understanding of the project’s context. The project included workshops, community consultations and the creation of twelve mini-murals, each a product of diverse images. These phases aimed to break down barriers and facilitate collaboration among community members, addressing power differentials and the complexities of community dynamics.
Interoception and emotional regulation in autistic children through an occupational therapy perspective: A literature review
Authors: Barmpagiannis Panagiotis , Baldimtsi Eleni
Published: February 2025

Understanding the interplay between interoception and emotional regulation is critical for advancing occupational therapy practices for autistic children and adolescents. These individuals often experience interoceptive processing difficulties, hindering emotional management, daily functioning, and societal participation. This review examines the relationship between interoception and emotional regulation and addresses relevant interventions to support these skills. To explore the relationship between interoception and emotional regulation and identify effective interventions that can inform occupational therapy practices for autistic individuals. A comprehensive search was conducted across three central electronic databases— PubMed, PsycINFO, and Google Scholar to identify studies that examined the relationship between interoception and emotional regulation in individuals with autism. Studies were conducted from 2013 to 2024. The search combined database-specific controlled language with keywords related to interoception, emotional regulation, occupational therapy, and autism spectrum disorder. A total of 49 studies meeting inclusion criteria were included. The review highlights the intricate connections between interoception and emotional regulation in autistic children, emphasizing challenges such as alexithymia and reduced interoceptive awareness. Interventions include cognitive behavioral therapy, mindfulness-based approaches, and specialized programs like the Aligning Dimensions of Interoceptive Experience and the Interoception Curriculum. These strategies can potentially enhance emotional well-being, self-regulation, and occupational engagement. Interoception and emotional regulation are foundational elements in the clinical reasoning of occupational therapists. Tailored, neurodiversity-affirmative interventions can promote emotional resilience and improve participation in daily activities for autistic individuals. Further research is necessary to develop inclusive and ethically aligned interventions that meet the needs of both clinicians and autistic people. This study underscores the significance of integrating interoceptive-based interventions into therapeutic practices to address emotional regulation challenges in autistic children, fostering their holistic development and societal inclusion.
DIÁLOGO ENTRE OS MÉTODOS EDUCACIONAIS DE PAULO FREIRE E CARL ROGERS
Authors: Branco Paulo Coelho Castelo , Monteiro Paulo Souza , Felix Lucas Matias
Published: February 2025

Paulo Freire foi um autor influente nos campos da Educação e da Psicologia, ao passo que Carl Rogers mencionou a obra "Pedagogia do Oprimido" para refletir os trabalhos terapêuticos grupais com minorias sociais exploradas. Objetivamos analisar teoricamente os métodos educacionais de Freire e Rogers, para estabelecer um diálogo comparativo entre ambos. Investigamos cada método conforme o seu objetivo, a problemática que lhe gerou e a operacionalização de sua proposta de aprendizagem. Estabelecemos, posteriormente, algumas interlocuções entre as duas perspectivas, ponderando suas convergências e divergências. Concluímos que ambos os métodos são ativos em suas propostas humanistas de aprendizagem pela experiência libertadora. Questionamos, entretanto, até que ponto um pode ser aplicado como ferramenta complementar do outro.
VIOLÊNCIA DOMÉSTICA: SIGNIFICADOS E REPRESENTAÇÕES CONSTRUÍDAS POR MULHERES VITIMIZADAS
Authors: Gregoleti Antonia de Fátima Mota , Paula Alexandre da Silva de , Kodato Sérgio
Published: February 2025

Esta pesquisa teve como objetivo: investigar a história de vida de sete mulheres vítimas da violência doméstica e identificar as formas pelas quais elas significam e representam o fenômeno. Referenciou-se no método de análise das representações sociais, sistematizado por Moscovici, Jodelet, Farr. As informações foram coletadas numa Delegacia Especializada de Defesa da Mulher, localizada em um município do interior do Estado de São Paulo. Os procedimentos da pesquisa envolveram a realização de entrevistas semiestruturadas em profundidade e um levantamento quantitativo nos Boletins de Ocorrência. Enquanto resultados, pode-se afirmar que as mulheres compartilharam histórias de sofrimento, submissão e resistência à violência desde a infância. Na ausência de infraestrutura e suporte para o enfrentamento, as denúncias são arquivadas, o que dificulta a punição jurídica aos agressores. A ingestão de álcool pelos companheiros foi significada como fator desencadeador dos conflitos e agressões, justificando novos episódios de angústia e dor.
Impuro, imperfeito, impermanente
Authors: Gonçalves Fábio Tibúrcio , Ribeiro Nunes Tiago
Published: February 2025

Partindo-se de breves considerações introdutórias a respeito da transição epistemológica do sujeito cartesiano para o sujeito freudiano, com ênfase na ruptura e na subversão do discurso psicanalítico como produção de um novo saber no limiar do século XX, o presente artigo pretende fazer uma análise de trechos da letra da canção “Vivo”, gravada no primeiro álbum ao vivo intitulado “In Cité” (2004) do cantor e compositor brasileiro Lenine (1959) que num engenho de síntese poética, lança-se no arrolando de uma série de palavras adjetivas, a princípio desconexas e ilhadas, para, ao final, compor o retrato ontológico de um sujeito marcado pela fragmentação de seu eu e que, a despeito dessa fragmentação e incompletude, continua vivo,estranho, deslocado e aqui presente.
Transtorno de Déficit de Atenção e Hiperatividade na clínica psicanalítica
Authors: Verissimo Juliana mistrini , Dos Reis Maria Elizabeth Barreto Tavares
Published: February 2025

O Transtorno de Déficit de Atenção e Hiperatividade (TDAH) tem sido a descrição diagnóstica mais utilizada para explicar os comportamentos considerados inadequados das crianças, sendo o uso do medicamento oferecido como principal meio de intervenção, apesar das possíveis consequências ao desenvolvimento infantil. Realizou-se uma revisão narrativa sobre o tema, do ponto de vista da psicanálise, com o objetivo de compreender como tem sido o olhar dos profissionais que se utilizam da teoria psicanalítica no atendimento à criança que recebe o diagnóstico de TDAH. Foi possível perceber a necessidade de melhor compreender as questões emocionais subjacentes à manifestação dos sintomas. Além disso, verificou-se que a clínica psicanalítica tem sido utilizada como uma alternativa favorável, pois considera a subjetividade da criança e suas relações parentais e sociais.
Psicoterapia para pacientes borderline, engajamento e prognóstico: a perspectiva de psiquiatras e psicólogos
Authors: Adami Alessandro , Portella Marilene Rodrigues , Portella Marilene Rodrigues , Fianco Dias Luis Francisco
Published: February 2025

O Transtorno da personalidade borderline causa dificuldades em estabelecer relações, vínculos interpessoais e organizar a própria vida. Estes pacientes possuem sintomas diversos, humor instável, impulsividade, dentre outros. Objetivou-se conhecer o processo terapêutico utilizado em pacientes com transtorno da personalidade borderline, na perspectiva dos profissionais de saúde. Trata-se de um estudo exploratório descritivo de abordagem qualitativa realizado na cidade de Passo Fundo, Rio Grande do Sul com três Psiquiatras e quatro Psicólogos. Para coleta de dados foram utilizadas entrevistas semiestruturadas, de duração variável com um roteiro norteador como ponto chave. Os dados foram analisados seguindo a técnica de análise temática, O resultado indica a utilização da psicanálise e psicologia cognitivo-comportamental, com foco nas necessidades do paciente, o ambiente e a confiança.
vínculo terapêutico como ferramenta efetiva para a terapia psicanalítica
Authors: Costa Jedaias da Costa e
Published: February 2025

Dentro do contexto de psicoterapia é muito válido salientar sobre a importância da aliança terapêutica, tendo em vista que o desenvolvimento do vínculo entre analisando e analista pode propiciar mudanças efetivas na terapia. O objetivo desta pesquisa é mostrar, através de um referencial bibliográfico, como o vínculo se manifesta na psicoterapia de forma a contribuir com as mudanças e aquisição de insight do analisando, desta forma, tornando a psicoterapia um processo efetivo. Além disso, mostrar como a efetivação da aliança entre analista e analisando interfere no avanço desta relação e como isto pode contribuir para o sucesso ou fracasso da terapia. Buscamos explanar sobre os diversos tipos de vínculo estabelecidos durante o processo terapêutico, bem como analisar as alterações feitas através da aliança terapêutica em atendimentos com base psicanalítica, para assim versar sobre como um vínculo bem estruturado pode efetivar a terapia. Por fim, buscamos trazer esclarecimentos acerca das alterações do vínculo e como este é estruturado para Pichon-Rivière.
Fenomenologia e relação terapêutica
Authors: Stenzel Lúcia Marques
Published: February 2025

A fenomenologia vem auxiliando pesquisadores a confrontar o formato tradicional de investigação da psicologia, rejeitando uma interpretação teorizante e objetificante dos fenômenos psicológicos. No entanto, o debate metodológico-empírico produz divergências entre os pesquisadores brasileiros. Este artigo apresenta uma revisão integrativa da literatura nacional em Psicologia acerca da forma como a relação terapêutica tem sido investigada em pesquisas que se fundamentam epistemologicamente na fenomenologia. Foi realizado um levantamento em bancos de dados virtuais (BVS, PePsic, SciELO, Portal Capes) em busca de artigos de estudos empíricos, publicados nos últimos dez anos, que tinham como foco a análise da relação terapêutica em termos de processo psicoterápico, independentemente da abordagem teórica. Dos 200 artigos encontrados, 16 foram selecionados para a leitura integral, sendo que somente dois se fundamentam epistemologicamente na fenomenologia. Os resultados e conclusões apontam para a escassez de trabalhos empíricos sobre a relação terapêutica e para as contribuições da fenomenologia na compreensão da relação terapêutica nos processos psicoterápicos. Os estudos encontrados propõem uma reflexão sobre a dimensão ontológica da intersubjetividade, rompendo com uma visão monológica do processo psicoterápico e apresentam contribuições sobre a possibilidade de investigação empírica da relação terapêutica fundamentadas epistemologicamente na fenomenologia.
Historiofotía del despojo de la Araucanía en el cine temprano chileno
Authors: Esparza Fica Anisell
Published: February 2025

El reciente hallazgo y publicación de documentos extraviados de uno de los primeros largometrajes chilenos, La agonía de Arauco o El olvido de los muertos (1917), ha pro-porcionado nuevas perspectivas para el análisis discursivo e histórico de este patrimonio cinematográfico. En el pre-sente artículo se examina la narrativa del filme dirigido por Gabriela Bussenius, a partir de la lectura de los guiones, poniendo énfasis en la concomitancia del argumento con los acontecimientos del despojo hacia el pueblo mapuche. Se corrobora la presencia de hechos verídicos que formaron parte del pillaje de la época y las simbologías del racismo imperante en el país debido a la Ideología de Ocupación. Asimismo, se evidencia el paralelismo entre la realidad de los colonos chilenos (Isabel) y la de los habitantes ancestrales del territorio mapuche (Catrileo), comprobando que el argumento del filme se construye sobre una contraposición melodramática de personajes, en la cual sus contextos se presentan como opuestos y en conflicto.
O cinema queer brasileiro e a desestabilização da masculinidade hegemônica nos grandes centros urbanos
Authors: Wandscheer João Paulo , De Souza Rossini Miriam
Published: February 2025

Este artigo consiste em uma análise da cidade como um espaço público que nos possibilita compreender tensionamentosque a homossexualidade provoca em relação à masculinidade hegemônica, através dos longas-metragens Tinta Bruta (2018) e Corpo Elétrico (2017). Será adotada como metodologia a análise fílmica (Aumont e Marie, 2004) e a atenção do trabalho estará voltada à narrativa das cenas em que ocorrem festas em meio à rua. As discussões sobre a construção do espaço fílmico terão como base o pensamento de Bordwell (1995), Gaudreault e Jost (2009) e Costa (2013a e 2013b). O conceito de masculinidade hegemônica terá como referência Connel e Messerschmidt (2013). Para a compreensão do histórico de reivindicações por direitos de grupos minoritários com sexualidades dissidentes, bem como a relação dos grandes centros urbanos com a trajetória de tais lutas políticas, este trabalho apresentará como embasamento teórico as obras de Lacerda (2015), Lacerda Júnior (2015), Nagime (2016), Lopes (2016), Louro (2018), Galuppo (2019), Ramalho (2020), Quinalha (2022) e Halberstam (2023). Como resultados, foi possível compreender que o cinema queer levanta provocações e questionamentos a paradigmas hegemônicos e, através de narrativas que se distanciam de uma tentativa de assimilação, os filmes analisados retratam possibilidades de reinventar cidades. A presença dos protagonistas de ambas as obras, em festas frequentadas por indivíduos heterossexuais, colocam em tensão preconceitos e desestabilizam a busca de personagens masculinos heterossexuais por dominação. A cidade, contudo, também é abordada como um espaço públicoonde a pluralidade e novas formas tanto de estar como entender o mundo encontram maneiras de resistir e viver com orgulho as diferenças.
A propósito del desarrollo del capitalismo argentino: el caso Vicentín. Entrevista al director de cine Andrés Cedrón sobre Cuellos blancos. El caso Vicentín (2024)
Authors: Nicola Mariné , Ramos Hugo
Published: February 2025

Desgrabación entrevista: Andrés Cedrón.
Entrevistado: Andrés Cedrón (AC).
Entrevistadores: Mariné Nicola (MN) y Hugo Ramos (HR).
Duración: 1 hora, 6 minutos
The ethical principles of the Portuguese psychologists: An evaluation after eleven years
Authors: Ricou Miguel , Marina Sílvia , Sá Eduardo
Published: February 2025

Several changes were implemented in the psychology profession after the Order of Portuguese Psychologists was created. A set of ethical principles to guide psychological practice was established. Eleven years after the several changes have been initiated in the professional practice in Portugal, this study analyzes whether there are changes in the valuation of the fundamental ethical principles of Portuguese psychologists. To this end, data from 2009 and 2020 was compared. On a grading scale from 1 to 5, Portuguese psychologists allotted the importance to each general principle. We found Competence and Responsibility decreased after 11 years, and Integrity and Beneficence and Non-Maleficence increased from 2009 to 2020. We also found that Psychologists with more experience valued more the Respect for people’s rights and dignity, Integrity, and Beneficence and non-maleficence than those with lesser years of experience. Order of Portuguese Psychologists and the existence of an ethics code seems to have improved the professional identity
Posttraumatic stress disorder, childhood trauma and health behaviors in victims of intimate partner violence
Authors: Gomes Solange Costa , Costa Eleonora Cunha Veiga
Published: February 2025

Intimate partner violence (IPV) is considered a serious public health problem and a significant risk factor for women’s health. In Portugal, studies focusing on the investigation of post-traumatic stress disorder (PTSD), childhood trauma, and health behaviors in victims of IPV are scarce. This research aimed to analyse the effects of childhood victimization and of IPV on PTSD symptoms and health behaviors in a sample of Portuguese women, including victims and non-victims of IPV (n=117). The participants’ ages ranged from 19 to 74 years (M=37.76; SD=14.85). Differences between women with and without IPV in childhood trauma, PTSD symptoms, and health behaviors were also examined. Results indicated that women who are victims of IPV experience higher levels of childhood abuse and PTSD symptoms and engage in fewer health behaviors compared to non-victimized women. Childhood physical and emotional abuse along with psychological aggression in adulthood are the strongest predictors of PTSD symptoms. Childhood sexual abuse and sexual coercion in adulthood contribute to a lower engagement in health behaviors. This study underscores the impact of IPV on mental health and highlights the need to design effective interventions that prevent future revictimization and promote healthy behaviors among victims.