bluuebunny/crossref_metadata_2025 huggingface dataset:
Social Media Engagement and Disclosure in the Iranian Setting
Authors: Koohikamali Mehrdad , Kermani Hossein , Rabiei-Dastjerdi Hamidreza
Published: January 2024

This study investigates how disclosure of different types of private
information about self, others, and non-personal information may be
influenced by privacy concerns and its impact on social media
engagement. We propose that the privacy concerns for self and privacy
for others have relationships with self-disclosure and disclosure of
information about others. Additionally, we investigated the relationship
between information disclosure (about self, others, and non-personal)
with social media engagement. We focus on social media usage by the
Iranian people. The Iranian government has many extreme restrictions on
the use of Internet and social media by its citizens. The recent
national protests in Iran provide a good opportunity to investigate
social media usage by the Iranians. The survey data was collected from
Iranian participants (n=379), and we empirically tested the proposed
research model. We found non-personal sharing has a positive
relationship with self-disclosure, disclosure about others, and
engagement. Furthermore, results indicate significant negative
relationships between privacy concern for self and self-disclosure and
privacy concern for others and disclosure about others.
A unique human cord blood CD8 + CD45RA + CD27 + CD161 + T cell subset identified by flow cytometric data analysis using Seurat
Authors: Ni Duan , Reyes Julen Gabirel Araneta , Santner-Nanan Brigitte , Pinget Gabriela Veronica , Kraftova Lucie , Ashhurst Thomas M. , Marsh-Wakefield Felix , Wishart Claire Leana , Tan Jian , Hsu Peter , King Nicholas Jonathan Cole , Macia Laurence , Nanan Ralph
Published: January 2024

Advances in single-cell level analytical techniques, especially
cytometric approaches, have led to profound innovation in biomedical
research, particularly in the field of clinical immunology. This has
resulted in an expansion of high-dimensional data, posing great
challenges for comprehensive and unbiased analysis. Conventional manual
analysis is thus becoming untenable to handle these challenges.
Furthermore, most newly developed computational methods lack flexibility
and interoperability, hampering their accessibility and usability. Here,
we adapted Seurat, an R package originally developed for single-cell RNA
sequencing (scRNA-seq) analysis, for high-dimensional flow cytometric
data analysis. Based on a 20-marker antibody panel and analyses of T
cell profiles in both adult blood and cord blood, we showcased the
robust capacity of Seurat in flow cytometric data analysis, which was
further validated by Spectre, another high-dimensional cytometric data
analysis package, and conventional manual analysis. Importantly, we
identified a unique CD8 T cell population defined as
CD8 CD45RA CD27
CD161 T cell, that was
predominantly present in cord blood. We characterized its
IFN-γ-producing and potential cytotoxic properties using flow cytometry
experiments and scRNA-seq analysis from a published dataset.
Collectively, we identified a unique human cord blood CD8
CD45RA CD27
CD161 T cell subset and
demonstrated that Seurat, a widely used package for scRNA-seq analysis,
possesses great potential to be repurposed for cytometric data analysis.
This facilitates an unbiased and thorough interpretation of complicated
high-dimensional data using a single analytical pipeline and opens a
novel avenue for data-driven investigation in clinical immunology.
Immobilizing Triphenylamine with Photoredox Inert Sr2+ Forming Sr-MOF with Controlled Electron Migration for Photocatalytic Oxidation of Thiols to Disulfides
Authors: Wang Ying-Xia , Zheng Fang , Song Dong-Xia , Niu Bao-Yue , Deng Lu-Qian , Zhang Xian-Ming
Published: January 2024

The photocatalytic oxidative coupling of thiols is one of the most
popular methods to synthesis the disulfides. Triphenylamine and its
derivatives (TPAs) are promising for the above reaction, but suffer from
the easy polymerization and difficult separation. To overcome these
obstacles while controlling the photogenerated electrons transfer
directly to target substrates, herein, we constructed one TPA-based
metal-organic framework (MOF), (Me2NH2)[Sr(TCPBA)]·2DMA·3H2O (1), by
direct self-assembly of tris(4′-carboxybiphenyl)amine (H3TCPBA) and
photoredox inert strontium ion (Sr2+). DFT calculations revealed that
the valence band maximum (VBM) and the conduction band minimum (CBM) are
mainly located on TCBPA3-, successfully inhibiting the undesirable
electron migration to metal nodes. Experimental results indicated that 1
displays superior performance than homogeneous H3TCPBA, which may result
from the abundant π···π and C-H···π interactions between the
well-arranged TCBPA3- and the build-in electric field between the
anionic framework and the Me2NH2+. This work highlights that
immobilizing TPAs into MOFs is one promising approach to design
heterogeneous photocatalysts for the synthesis of disulfides by
oxidative coupling of thiols.
Exploring potential ferroptosis biomarkers and immune infiltration features in rheumatoid arthritis through bioinformatics analysis
Authors: You Wenkang , Chen Xiaoqing , Du Zhiyin , Lin Yanbin , Zhang Lv , Huang Zehui , Zeng Rongdong
Published: January 2024

Although an increasing number of studies suggest that ferroptosis is
involved in the development of rheumatoid arthritis, the specific
mechanisms by which this phenomenon occurs remain elusive. The aim of
this study was to investigate the genes associated with ferroptosis in
RA and their mechanisms of action. We downloaded four gene expression
profiles from the Gene Expression Omnibus (GEO) database. After
identifying differentially expressed genes (DEGs) between rheumatoid
arthritis (RA) synovial tissues and control synovial tissues, basic
bioinformatics analyses were performed, including functional annotation,
protein-protein interaction (PPI) network, hub gene screening,
pharmacogenetic prediction and TF-mRNA-miRNA regulatory network
construction. In addition, we analyzed RA immune infiltration according
to the CIBERSORT algorithm to assess the relevance of infiltrating
immune cells in RA patients. Finally, we identified four hub genes,
including CDKN1A, epidermal growth factor receptor, ATF3, and JUN genes,
and a total of 92 miRNAs and 14 transcription factors were predicted to
form a regulatory network with the hub genes, and 8 drug candidates were
screened. These findings may help
us to better understand RA pathophysiological changes in basic
research, as well as provide new evidence for clinical transformation.
We believe that the drug candidates obtained in this study might be
helpful for effective therapeutic in RA.
Fish skin-inspired Janus hydrogel coating for drag reduction
Authors: Zhang Yurong , Li Lijun , Wang Ruteng , Liu Junwei , Lin Zhen , Li Gang , Liu Hongtao , Lei Yifeng , Xiong Yunhe , Zhao Yan , Xue Longjian
Published: January 2024

In nature, fishes have evolved functional skins with effective
hydrodynamic performance to reduce water resistance, anti-fouling,
facilitating predation and escaping from predators. Although a large
number of fish scale-inspired structured surfaces have been explored,
the incorporation of mucus on the structured surfaces has been largely
ignored. Inspired by the skins of Osteichthyes fishes, a Janus hydrogel
coating (JHC) is successfully prepared by a two-step UV light
irradiation at room temperature. The bottom side of JHC (STH) achieves a
shear adhesive strength of 103.3 ± 17.5 kPa and can strongly adhere to a
large variety of surfaces, including metals, ceramic and polymers. The
top surface of JHC (SLH) replicates the structure of cycloid scales,
while the nature of hydrogel mimics the mucus on fish skin. SLH
possesses prominent mechanical, anti-swelling, anti-fouling and drag
reduction properties. The design strategy for JHC has potential
applications in numerous fields, like, pipeline transportation,
bioengineering, and shipping industry.
Technology Integration through Additive Manufacturing of Wind Turbine Blade Tips
Authors: Houchens Brent C. , Sproul Evan , Berg Jonathan , Caserta Paolo G. , Hernandez Miguel H. , Houck Daniel , Lopez Helio , Maniaci David , Monroe Graham , Paquette Joshua , Rodriguez Sal , Tilles Julia N. , deVelder Nathaniel , Williams Michelle , Westergaard Carsten H. , McIntosh Trey , Payant James A. , Wetzel Kyle K.
Published: January 2024

The Additively-Manufactured, System-Integrated Tip (AMSIT) project is
leveraging the flexibility of 3D printing to integrate several
technologies in a wind turbine blade tip, while reducing the levelized
cost of electricity (LCOE) produced. The design integration is
demonstrated for a 200 kilowatt-scale turbine with 13-meter blades, with
the outer 15% of the blade replaced with a 3D-printed design.
Aerodynamic performance is enhanced through inclusion of a winglet and
surface texturing, both challenging for traditional manufacturing.
Longevity and durability is improved through integrated lightning and
leading edge erosion protection. Increased power, reduced repair
frequency, and ease of repair through blade modularity all contribute to
reduced LCOE. Cost models are are extended to modern megawatt-scale
designs to estimate the impact of the technology at scale, demonstrating
the potential to reduce LCOE very significantly for modern onshore
turbines, with even higher potential savings offshore.
New insight into the molecular phylogeny and morphometrics of the genus Acer L. (Sapindaceae) native to Turkey
Authors: ACAR Pelin , Seki Nagihan , Bani Barış , Demircioğlu Nurcan , DUMAN Hayri , Kaya Zeki
Published: January 2024

This study investigates the phylogenetic relationships, taxonomic
status, and morphometric analysis of 21 naturally occurring taxa of the
Acer genus in Turkey. A comparative analysis was conducted using
18 informative morphometric characters and DNA sequence data from the
cpDNA ndhF, trnL-F, and nrDNA ITS gene regions. To date, there
has been no comprehensive molecular and morphometric study conducted on
the genus in Turkey. Factor Analysis of Mixed Data (FAMD) was performed
on selected morphometric traits, revealing highly distinctive
characteristics for the Acer genus at the series, sectional, species,
and sub-species levels, including Dim1 traits such as leaf
length, leaf width, petiole length, loculus length, loculus width, fruit
wing length, and fruit length, as well as Dim2 traits such as
pubescence of leaf beneath, margin, latex, and form of nutlets, along
with inflorescence, bud, and shoot features. The nuclear ITS and
chloroplast trnL-F gene regions also exhibited significant
diversity and provided valuable information. Phylogenetically,
Acer species in Turkey showed close relationships with species
native to the Eurosiberian phytogeography. The results of the
phylogenetic and morphometric analyses offered reliable taxonomic
clarifications for the first time, specifically for the species
Acer tauricolum, A. divergens (endemic to Turkey), A.
orthocampestre, and A. cappadocicum subsp. cappadocicum.
The chloroplast lineage of Acer species native to Turkey appears
to have originated in the late Oligocene and experienced significant
diversification during the Miocene. The majority of Acer
phylogenetic positions in Turkey are likely influenced by the habitats
created by the northern mountain ranges.
Effects of polyphenols from Polygonum multiflorum Caulis on multiple targets of obesity-related disorders
Authors: Shen Chun-Yan , Hao Yun-Fang , Hao Zhan-Xi , Xie Hong , Jiang Jian-Guo
Published: January 2024

Background and Purpose: Polyphenols of Polygoni Multiflori Caulis (PMC)
have been widely consumed as food supplements for lowing lipid, while
their anti-obesity effects were poorly understood. Experimental
Approach: Several polyphenol-rich extracts including 75% ethanol
(ETOH), chloroform (CHL), ethyl acetate (EA), n-butanol (n-Bu) and water
(W) fractions, composed of emodin, physcion, apigenin or
2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside at different ratios,
were isolated from PMC. Their effects on lipase activity and 3T3-L1
preadipocytes differentiation were comparatively investigated. Their
effects on obesity-related disorders were comparatively investigated
using various models both in vitro and in vivo. Key Results: EA and n-Bu
extracts with greater anti-obesity potential were screened for further
studies to reveal the underlying mechanisms. EA and n-Bu extracts
treatment significantly modulated the antioxidant response and lipid
accumulation in Caenorhabditis elegans. High fat diet (HFD)-induced
changes of weight gain, liver steatosis and epididymal adipose tissues
hypertrophy in mice were also significantly reversed by EA and n-Bu
extracts supplementation. Moreover, EA and n-Bu extracts intervention
increased the microbial diversity, reshaped the microbiota structure and
enhanced the relative abundance of Bifidobacterium. N-Bu extracts
supplementation significantly decreased the ratio of
Firmicutes/Bacteroidetes and the abundance of Lactobacillus, while EA
extracts treatment markedly reversed HFD-induced increase of
Erysipelotrichaceae abundance. Conclusions and Implications: Dietary
supplementation of polyphenols from PMC exhibited promising anti-obesity
effects and the chemical difference could partially explain their
bioactivity discrepancy.
Global Dynamics of a Class of Nonclassical Diffusion Equations in Solids
Authors: Luo Hongdong , Wang Ruijing
Published: January 2024

This paper is concerned with the first initial and boundary value
problem of a class of nonclassical diffusion equations in solids. We
first establish uniform decay estimates which are independent of the
viscosity coefficient and prove some new existence and uniqueness
results for solutions of the problem under some appropriate conditions.
Then we show that it has a global pullback attractor. In the periodic
case, we point out that the pullback attractor is actually periodic and
forward attracting; furthermore, the problem has at least a periodic
solution.
Inpatient Utility of Pro-BNP in COVID-19: A Single Center Experience
Authors: Ali Saif , Nandkeolyar Shuktika , Doctorian Tanya , Chatta Payush , Stoll Shaunrick , Park Eunice , Stutzman Reece , Foster Kristoff , Fabbri Sharon , Abramov Dmitry , Stoletniy Liset , Sakr Antoine
Published: January 2024

Abstract: Aims: The purpose of this study was to identify serologic and
clinical factors in SARS-Coronavirus 2 (SARS-CoV-2) patients which may
correlate and predict need for mechanical ventilation and mortality.
Furthermore, we sought to determine if a diagnosis of heart failure and
elevations in Pro B-Type Natriuretic Peptide (pro-BNP) predicted
clinical endpoints. Methods: 135 patients age 18 or older admitted to
Loma Linda University Medical Center with SARS-Co-2 between March 16,
2020 and June 8, 2020 were categorized by demographic data (age, race,
and gender), clinical parameters (temperature, heart rate, blood
pressure, and peak fraction of inspired oxygen (FiO2)), and serologic
markers pro-BNP. A regression analysis was done to correlate need for
mechanical ventilation and mortality. Results: In univariate analysis,
elevated pro-BNP correlated with need for mechanical ventilation
(p=0.006) and mortality (p=0.001). In multivariate analysis, elevated
pro-BNP was significantly associated with requiring intubation (OR 4.5,
p=0.03). Each year of age (OR 1.08, p=0.006) and unit of BMI (OR= 1.05,
p=0.03) were significantly associated with increased mortality. In
patients without a diagnosis of heart failure, average pro-BNP was
elevated (1141.66 ng/dL) however it did not correlate with need for
intubation or mortality. Conclusions and clinical implications:
Coronavirus disease-19 (COVID-19) is associated with increased mortality
and worse clinical outcomes in patients with heart failure. Despite a
lack of heart failure diagnosis, elevated markers such as NT-Pro-BNP
have been associated with increased mortality in COVID-19 hospitalized
patients. Although no clear ‘cut-off’ has been described, our study
findings suggest elevated pro-BNP may be a useful marker for disease
severity and respiratory complications of COVID-19 patients with
possible cardiac involvement. Similar to other single center findings,
our study demonstrates worsened clinical outcomes for patients with
advanced age and elevated body-mass index.
SIMPLIFIED MICROMECHANICS APPROACH FOR MODELING AND ANALYSIS OF UD COMPOSITES PERFORMANCE
Authors: Godara S. S. , Mahato P. K.
Published: January 2024

A simplified micromechanical approach is used for the modelling and
analysis of unidirectional (UD) composite performances. In this paper,
the influence of volume fraction and constituent properties on the
effective longitudinal, transverse, and shear properties of
unidirectional composites are investigated. These effective properties
are determined using the micromechanical approach, which is based on
mathematical modelling using the rule of mixtures. Four different types
of unidirectional composites such as T300/BSL914C, IM7/8511-7,
T300/PR319, and S2-Glass/epoxy were used for analysis purposes. The
method was validated with existing experimental results. The results are
dependent on an array of parameters, such as the orientation of fibers,
the volume fractions of fibers, array of fibers and the material
properties of their constituents. Further, this micromechanical method
might be used with other reinforcing fibers for the prediction of
properties of UD, hybrid and other composites architectures.
Establishment and validation of a duplex, real-time PCR assay for detection of African swine fever virus
Authors: Zhan Yang , Zhang Luhua , Lin Yuan , Cai Yunfeng , Zou Yawen , Hao Zhiyou , Luo Zhanhua , Wang Naidong , Deng Zhibang , Yang Yi , Wang Changjian , Yang Qing
Published: January 2024

The outbreak of African swine fever (ASF) in China in 2018, caused a
huge economic loss on the pig industry. To accurately diagnose clinical
infection with ASF virus (ASFV), we developed a duplex
TaqMan-probe-based real-time PCR for the simultaneous detection of two
discontinuous genes in the virus genome to avoid false-negative in the
clinical diagnosis. Two sets of ASFV-gene specific primers, along with
two TaqMan fluorescent probes were designed to target the conserved
region of B646L and B438L genes, respectively. The method showed high
sensitivity and specificity, with a detection limit of 10 copies/μl
without cross-reactions with other pathogen genomes of CSFV, PRRSV,
PEDV, PRV, PPV and PCV2. 269 clinical swine samples were tested using
this method, and the results of 60 samples showed excellent consistency
(95.0%) with a commercial kit. This method will greatly improve the
efficiency for surveillance of ASFV and can help reduce economic losses
to the swine industry, which also benefits animal and public health.
Evaluation of Clinical and Laboratory Findings of Patients with COVID-19 Over 50 Years Old: A Retrospective Study
Authors: Şener Aslı
Published: January 2024

Background: Individuals of any age can acquire SARS-CoV-2 infection,
although adults of middle age and older are most commonly affected, and
older adults are more likely to have severe disease. Therefore, the
management of patients with COVID-19 over the age of 50 is very
important. Aims: In this study, we aimed to investigate the
epidemiological, clinical, and laboratory findings of COVID-19 patients
over the age of 50 in a tertiary education and research hospital. Study
Design: Single-center retrospective cross-sectional study. Methods:
Patients older than 50 years of age who applied to the emergency service
with suspected COVID-19 and had positive nasopharyngeal PCR were
included in the study. The patients were divided into 3 groups according
to their ages (50-64 years, 65-79 years, and 80 years and over,
respectively). Demographic characteristics of the patients such as age,
gender, admission symptoms, clinical findings, laboratory results,
imaging results, and hospital follow-up periods were recorded. Results:
A total of 119 patients were included in the study. Seventy-five
patients were 50-64 years old (63.02%), 31 patients were 65-79 years
(26.05%), and 13 patients were over 80 years old (10.9%). It was
observed that fever and respiratory distress gradually increased with
age (p = 0.019, p = 0.029). Considering the laboratory values between
the groups, statistically significant differences were found in terms of
anemia (p ˂ 0.05), lymphopenia (p ˂ 0.05), and the neutrophil/lymphocyte
ratio (p = 0.009). Positive computed tomography findings were detected
in 88 (73.9%) cases. The rates of service and intensive care
hospitalization increased with age and were highest in patients over the
age of 80 (p < 0.001). Conclusion: Age is an important risk
factor in COVID-19 disease. In COVID-19 disease, special protocols
should be established for elderly patients and studies involving larger
patient samples should be conducted.
Corticotropin-releasing hormone stimulates mast cell degranulation and proliferation in human nasal mucosa
Authors: Yamanaka-Takaichi Mika , Mizukami Yukari , Sugawara Koji , Sunami Kishiko , Teranishi Yuichi , Kira Yukimi , Paus Ralf , Tsuruta Daisuke
Published: January 2024

Background: Psychological stress exacerbates mast cell (MC)-dependent
inflammation, including nasal allergy, but the underlying mechanisms are
not well understood. Because the key stress-mediating neurohormone,
corticotropin-releasing hormone (CRH), induces human skin MC
degranulation, we hypothesized that CRH may be a key player in
stress-aggravated nasal allergy. The current study probes this
hypothesis in human nasal mucosa MCs (hM-MCs) in situ, using nasal polyp
organ culture, and tests whether CRH is required for murine M-MC
activation by perceived stress in vivo. Methods: Organ-cultured nasal
polyps were used as a surrogate tissue for human upper airway mucosa and
stimulated with CRH. In addition to quantitative immunohistomorphometry
and transmission electron microscopy, we investigated whether perceived
(restraint) stress activates murine M-MCs in vivo and whether this is
CRH-dependent. Results: CRH stimulation significantly increased the
number of hM-MCs, stimulated both their degranulation and proliferation
ex vivo, and increased SCF expression in human nasal mucosa epithelium
compared to controls. CRH also sensitized hM-MCs to further CRH
stimulation and promoted a pro-inflammatory hM-MC phenotype. The
CRH-induced increase in hM-MCs was abrogated by co-administration of
CRH-R1 specific antagonist antalarmin, CRH-R1 siRNA, or SCF-neutralizing
antibody. In vivo, both acute and chronic restraint stress significantly
increased the number and degranulation of murine M-MCs compared to
sham-stressed mice. This effect was abrogated by intranasal antalarmin.
Conclusion: CRH is a major activator of hM-MC in nasal mucosa, in part
via promoting SCF production, and CRH-R1 antagonists like antalarmin are
promising candidate therapeutics for nasal mucosa neuroinflammation
induced by perceived stress.
Clinical characteristics, risk factors and antiviral treatment of Influenza in immunosuppressed inpatients in Beijing, 2015-2020
Authors: Liu Yafen , Wang Yue , Mai Huan , Chen Yuanyuan , Liu Baiyi , Liu Yisi , Ji Ying , Cong Xu , Gao Yan
Published: January 2024

Background: Influenza infection was a vital threat to immunosuppressed
patients with longer viral shedding; however, data on these populations
in China are still lacking. We analyzed clinical characteristics, risk
factors and effect of antiviral therapy in these populations. Methods:
We analyzed 111 immunosuppressed inpatients tested positive for
influenza virus using RT-PCR during the 2015-2020 influenza season.
Univariate analysis and multivariate logistics analysis were used to
identify risk factors. Results: The most common immunosuppression type
was malignancies with chemotherapy 87.4% (97/111), then hematopoietic
stem cell transplantation 23.4% (26/111). The most common presenting
symptom was fever in 92.8% (103/111) patients, then cough 50.6%
(44/87). 14.4% (16/111) patients were admitted to ICU and 9.9%
(11/111) patients died. Combination and double dose of neuraminidase
inhibitors did not significantly reduce the admission to ICU and death.
Risk factors for admission to ICU were dyspnea, co-infection with other
infections and no antiviral treatment within 48 hours, and presence of
dyspnea and altered mental status were independently associated with
death through multivariate logistics analysis. Seasonal influenza
vaccination in preceding 12 months only took up 2.7% (3/111).
Conclusion: Fever and other classical symptoms of influenza may be
absent in immunosuppressed recipients, and conducting influenza virus
detection at the first time is a good choice for early diagnosis.
Immunosuppressed patients with dyspnea, altered mental status,
co-infection with other infections and no antiviral treatment within 48
hours are of note needed, because these people have high-risk to severe
cases. Inactivated influenza vaccination should be taken into account in
immunosuppressed patients.
High-quality and sustainable development of soil and water conservation vegetation in a water-limited region
Authors: guo zhongsheng
Published: January 2024

In the water-limited regions, there is a limit of vegetation restoration
because the short of soil water and people’s big need. However, there
are few reports on the limit of vegetation restoration. In this study,
non-native perennial Caragana shrublands was selected to establish
experimental plots with different densities, and cover degree, basal
diameter and plant height growth, soil water and precipitation was
investigated, soil water supply and soil water consumption at different
planting density was estimated and the relationships between planting
density and soil water, soil water carrying capacity for vegetation and
the potential for vegetation rehabilitation was analyzed. The results
showed that the degree of cover increases with increasing density under
other things being equal; the canopy interception increases with stand
density and both of them is an exponential relation; the surface runoff
decreases with increasing density and the relationship between them is a
logarithm relationship; the sediment charge in the runoff increases with
reducing density and the relationship between them can be expressed by a
logistic equation. Although the canopy closure increases and the effect
of the caragana forest on soil and water conservation strengthens with
increasing density, but there is a limit of vegetation rehabilitation.
When the cover degree of Caragana shrublands is more than the vegetation
restoration limit, the water-plant relationship should be regulated
based on soil water carrying capacity for vegetation to control soil
drying and realize high-quality and sustainable development of soil and
water conservation vegetation.
Spatial and Temporal Genetic Variation in Ethiopian Barley (Hordeum vulgare L.) Landraces as Revealed by Simple Sequence Repeat (SSR) Markers
Authors: Dido Allo , Assefa Ermias , Degefu Dawit , Krishna M. S. R. , Singh B.J.K , Tesfaye Kassahun
Published: January 2024

Ethiopia is a center of diversity for barley (Hordeum vulgare L.) and it
is grown across different agro-ecologies of the country. Unraveling
population structure and gene flow status on temporal scales assists an
evaluation of the consequences of physical, demographic as well as
overall environmental changes on the stability and persistence of
populations. Here, we examine spatial and temporal genetic variation
within and among barley landrace samples collected over a period of four
decades (1976-2017), using simple sequence repeat (SSR) markers. Our
objective was to evaluate spatial and temporal changes in barley
population connectivity associated with the closure of geographic origin
and time periods. Low to strong genetic diversity was observed among the
landraces and STRUCTURE, Neighbour joining tree and Discriminant
Analysis of Principal Component analysis revealed three clusters. The
cluster analysis revealed a close relationship between landraces along
geographic proximity with genetic distance increases along with
geographic distance. The grouping of landraces based on altitudinal
classes was influenced by geographic proximity. From AMOVA year
categories, it was observed that within population genetic diversity
much higher than between population genetic diversity and that the
temporal differentiation is considerably smaller. The low to strong
genetic differentiation between landraces from various geographic
origins could be attributed to gene flow across the region as a
consequence of seed exchange among farmers. Nevertheless, we found some
connectivity between changes in population dynamics as well as
contemporary gene flow. The results demonstrate that this set of SSRs
was highly informative and was useful in generating a meaningful
classification of barley germplasms. Furthermore, our data also suggest
that landraces are a source of valuable germplasm for sustainable
agriculture in the context of future climate change, and that in-situ
conservation strategies based on farmers use can conserve the genetic
identity of landraces while allowing adaptation to local-environments.
Locoregional treatment of fibrolamellar hepatocellular carcinoma in a child patient: a case report
Authors: Civan Caner , Simsek Duygu Has , Bay Sema Büyükkapu , Poyanli Arzu , Kebudi Rejin , Turkmen Cuneyt
Published: January 2024

Transarterial radioembolization (TARE) with Yttrium-90- microspheres is
a well-tolerated and efficient treatment option in adult patients with
unresectable primary and secondary liver malignancies. However, the use
of TARE has been reported in limited pediatric cases. We present a
twelve-year-old male with metastatic unresectable fibrolamellar
hepatocellular carcinoma who received multidisciplinary treatments
including chemotherapy, TARE, and transarterial chemoembolization with
remarkable regression of the hepatic lesions. The patient is alive with
the disease and a good quality of life for 33 months since diagnosis.
Dermatologists as frontline physicians against COVID-19
Authors: Aktaş Habibullah , Ertruğrul Gökşen
Published: January 2024

COVID-19 is a disease that mainly concerns the specialties of infectious
and chest diseases. However, there was such a big epidemic all over the
world that the experts of these specialties could not bear the patient
burden. Many countries ,including Turkey, across the world, physicians
of all specialties as well as preclinical doctors (residents) have taken
care of patients in the COVID-19 outpatient and inpatient clinics.
Dermatologists,too, started working as frontline physicians during the
pandemic.
Global solvability in a three-dimensional cross-diffusion system modeling urban crime propagation
Authors: Jiang Yongfeng
Published: January 2024

In this paper, we will focus on the no-flux initial-boundary value
problem for the three-dimensional cross-diffusion system
&u_t=\Delta
u-\chi\nabla\cdot(\frac{u}{v}\nabla
v)-uv+B_{1}(x,t),& x
\in\Omega,t>0,
&v_t=\Delta v+uv-v+B_{2}(x,t), &
x\in\Omega,t>0 with some
positive parameter \chi. Under some basic assumptions on
the parameter functions B_{1}, B_{2} and \chi
\in (0,\sqrt 3), we show that the system
possesses at least one global renormalized solution in case that all
model ingredients are radially symmetric with respect to the center of
\Omega.
Endocardial lead extraction with transesophageal echocardiography guidance for cardiovascular implantable electronic device (CIED) related infections
Authors: Han Qiaoyu , Feng Yi , Ju Hui , Jiang Yan , Ze Feng , Li Xuebin , Jiang Luyang
Published: January 2024

With the expanding use of cardiac implantable electronic device (CIEDs)
in older populations with more complicated conditions have bring about
higher rates of CIED infections. We report four cases of CIED infections
from single-center, who were extracted endocardial leads with intra-op
transesophageal echocardiography (TEE) monitoring and guidance. Three of
them had fatal complications as pulmonary embolism, pericardial
tamponade and tricuspid trauma. The other one was found a massive
vegetation detected by TEE not by pre-op transthoracic echocardiogram
(TTE), which could avoid intra-op embolism. Application of TEE helps
improving efficacy and safety by capacitating the operators to perform
the procedure, guiding them to better plans and to rapidly recognize and
manage relative complications. Thus, continuously intra-op TEE should be
one of the routine monitoring methods in this high-risk procedure.
Transient Myeloproliferative Disorder- Diagnosis and Management using Cytarabine: Case Series
Authors: Rahim Mahvish , Lajiness Jacquelyn , Saraf Amanda , Shukla Riddhi , Rose Rebecca
Published: January 2024

Transient Myeloproliferative Disorder (TMD) is a rare diagnosis that
clinicians should be aware of as early recognition and management is
vital. There is little guidance regarding the details of management for
TMD, with respect to dosing and duration of chemotherapy. We present our
management in two cases of TMD.
Bioinformatics Identification of Vascular Endothelial Growth Factors as a Therapeutic Target for Diabetic Nephropathy
Authors: yang qunfeng , Luo Ensi , Li Xiang , Hu Lingzhi , Yang Lili
Published: January 2024

Background: Vascular endothelial growth factor (VEGF) functions are
different in different tissues and organs and different functional
states. VEGF is highly expressed in early stage diabetic nephropathy
(DN) and in turn, lowly expressed in late stage DN. However, their roles
in renal progression in advanced diabetes remain unclear. The purpose of
this study was to determine the relationship between low VEGF expression
and the pathogenesis and prognosis of advanced DN. Methods: Our study
found significant differences in VEGF gene expression using data from
the Gene Expression Omnibus (GEO) database based on renal tissue samples
of DN and normal people. Then, we performed several bioinformatics
analyses to determine differentially expressed genes, Gene Ontology
(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment,
Gene-Set Enrichment Analysis (GSEA), and immune infiltration analysis on
DN samples to obtain information on VEGF genes. Finally, we constructed
protein-protein interaction networks, and screened hub-genes to refine
our results further. Results: We found 180 differentially expressed
genes through our bioinformatics analysis. They were also enriched in
inflammatory response, cell differentiation, cell chemotaxis,
proliferation, and apoptosis pathways. We found eight hub-genes by PPI
network analysis. Together with VEGF, they may play a key role in
regulating the development of DN. Conclusion: Our study revealed the
molecular mechanism of DN pathogenesis and provides evidence that a
decrease in VEGF expression in moderate-to-late DN could be related to
its clinical prognosis. VEGF may be expected to become a new target for
DN therapy based on this research.
NEPHRON SPARING SURGERY IN BILATERAL WILMS TUMORS WITH BOTRYOID GROWTH PATTERN
Authors: Mrad Chaima , Audry Georges , Tabone Marie-Dominique , POINTE Hubert DUCOU LE , L'HERMINE Aurore COULOMB , Irtan Sabine
Published: January 2024

Botryoid growth pattern (BGP) is a polypoid mass growing into the renal
pelvis, rarely seen in bilateral Wilms tumors (BWT) where it represents
a surgical challenge. We report our experience of nephron sparing
surgery (NSS) in three patients with BGP in BWT. Surgical en-bloc
removal was performed after calyx opening with no complications. The
histology of the BGP was Intra Lobar Nephrogenic Rest in all cases while
all Wilms tumors were of intermediate risk. One patient early recurred.
At a follow-up of 9 months, 22 and 23 years, all patients were alive
with a moderate renal insufficiency and hypertension.
New markers in the etiopathogenesis of bacterial sepsis: Intracellular glutathione and extracellular thiol/disulfide homeostasis
Authors: AVCIOĞLU GAMZE , Otal Yavuz , Erel Özcan
Published: January 2024

Introduction: The aim was to analyze intracellular oxidised glutathione
(GSSG)/reduced glutathione (GSH) and serum thiol/disulfide homeostasis
tests in bacterial sepsis (BS) to evaluate the contribution of oxidative
stress to etiopathogenesis. Methods: A total of 92 individuals,
including 49 patients and 43 healthy volunteers, were included in the
present study. The cases who had high probability of BS criteria defined
by Singer et al. and positive blood culture were included in the study.
The intracellular oxidised and reduced glutathione and thiol/disulfide
homeostasis [total thiol-native thiol-disulfide changes] tests were
used in the serum samples of 49 patients. Statistical analysis was
performed by comparing the results with the control group. Results: When
BS and control group were compared, GSH, total glutathione (GSH+GSSG),
native thiol and total thiol levels were significantly lower in BS group
compared to control group (p<.001). No significant differences
were detected between the two groups in terms of GSSG levels. CRP, PCT,
WBC and RDW levels were significantly higher in BS group than in control
group (p<.001). Conclusions: Total thiol and native thiol
levels in serum and intracellular glutathione levels decreased in BS.
Disrupted intracellular GSH/GSSG and serum thiol/disulfide homeostasis
might have effects on BS pathogenesis by increasing oxidative stress.
Intracoronary unfractioned Heparin induced life-threatening hemopericardium. The successful curative treatment with injection of adrenaline and tranexamic acid into pericardial space, First Definition.
Authors: Balaban Yakup
Published: January 2024

Background : Acute hemopericardium due to intracoronary heparin has not
been identified so far. Methods : A 57-year-old male patient was
admitted to our clinic and PCI was performed. Pericardial tamponade
developed with acute pain after intracoronary 100 u/kg unfractionated
heparin(UFH) administration. Pericardiocentesis was performed and the
high amount of hemorrhagic fluid was drained. Pericardial leakage could
not be stopped despite thrombocyte suspension and fresh frozen plasma
infusion. Tranhexamic acid 500 mg and 250 mcg adrenaline were
administered to the pericardial space with 50 cc isotonic dilution and
curative treatment was obtained. Computed Tomography, USG,
echocardiography, and laboratory tests did not reveal any specific
findings. Discussion : Twenty-two cases with hemopericardium following
100 U/kg heparin and PCI treatment were published with our case. Heparin
was not held responsible for any of these cases, but the given
antithrombocyte and thrombolytic agents had been. The common point of
all 23 cases with our case is heparin. In our opinion, this phenomenon
is a complication of heparin. Conclusion : If there is a chest pain or
hemodynamic impairment in the patient after intracoronary heparin, the
patient should be followed closely by echocardiography. If pericardial
effusion is observed, immediate protamine administration should be made
and plasma and platelet suspension should be given. Administration
tranexamic acid and adrenaline with 50 cc isotonic infusion into the
pericardial space may be considered for curative treatment.
Use of Anti-Inflammatory Drugs for the Treatment of Delayed Onset Muscle Soreness: A Systematic Review and Meta-Analysis
Authors: Nahon Roberto , Lopes Jaqueline Santos , Neto Anibal Magalhães , Machado Aloa , Cameron Luiz Carlos
Published: January 2024

Objective: To investigate the effectiveness of Anti-Inflammatory Drugs
in the treatment of delayed onset muscle soreness (DOMS). Design: A
systematic review and meta-analysis of randomized controlled clinical
trials (RCTs). Setting: The PubMed/MEDLINE, EMBASE, SPORTDiscus, Scielo
and CENTRAL databases were searched up until August 3, 2020. Studies
that included 1) used an RCT design; 2) evaluated the effectiveness of
anti-inflammatory drugs (NSAIDs) in treating DOMS; and 3) drugs should
have been applied after exercise. Participants: Healthy participants who
were free of acute or chronic diseases. There were no restrictions
regarding the participants’ age, sex, level of activity. Interventions:
Anti-Inflammatory Drugs (NSAIDs). Main Outcome Measures: Improvement of
late muscle pain. Results: In total, 26 studies (n = 934 participants)
were eligible for inclusion. The results of the meta-analysis fail to
show superiority between the use and non-use of NSAIDs in the
improvement of late muscle pain, as statistically significant
differences were not verified (21 studies; standard mean difference
(SMD)= 0.02; 95% confidence interval (CI) -0.58, 0.63; p=0.94;
I2=93%). The quality of the synthesized evidence was very low according
to the Grading of Recommendations Assessment, Development and Evaluation
(GRADE) criteria, and there was significant heterogeneity among the
included studies. Conclusion: Use of NSAIDs in the management of DOMS
does not appear to be superior to a control condition and/or placebo.
However, these interpretations should be analysed with caution since the
type of NSAID, dose/response relationship and volume/intensity of the
effort made to induce different kinds of muscle damage varied across
studies.
A new blow up criterion for the 3D magneto-micropolar fluid flows without magnetic diffusion
Authors: Chen Dongxiang , Liu Qifeng , Chen Xiaoli
Published: January 2024

This note obtains a new regularity criterion for the three-dimensional
magneto-micropolar fluid flows in terms of one velocity component and
the gradient field of the magnetic field, i.e. the weak solution
$(u,\omega,b)$ to the magneto-micropolar fluid flows
can be extended beyond time $t=T$, provided if
$u_3\in
L^{\beta}(0,T;L^{\alpha}({\R}^3))$
with
$\f2{\beta}+\f3{\alpha}\leq\f34+\f1{2\alpha},\alpha>\f{10}3$
and $\nabla b\in
L^\f{4p}{3(p-2)}(0,T;\dot{M}_{p,q}({\R}^3))$
with $1
Analysis of Parental Abnormal Chromosomal Karyotype and Subsequent Live Births in Chinese Couples with Recurrent Miscarriage
Authors: Li Shan , Chen Mei , Zheng Peng-Sheng
Published: January 2024

Objective: To identify the influence of parental abnormal chromosomal
karyotype on the pregnancy outcomes of recurrent miscarriage (RM)
couples. Design: Retrospective cohort study. Setting and Population:
3235 RM couples who experienced two or more miscarriages before 20 weeks
were diagnosed in our tertiary referral unit of a university hospital
during 2008 to 2018. Main Outcome Measure(s): The frequency and
distribution of 121 carriers were described. Pregnant RM carriers (n=55)
and non-carriers (n=229) were compared in the etiological factors and
pregnancy outcomes. Result(s): Chromosome aberration was detected in 121
(3.74%) among 3235 RM couples which included 75 female and 46 male
cases at an individual level. 101 cases were structural aberrations
including balanced translocations in 46(38.0%) cases, Robertsonian
translocations in 13(10.7%) cases, inversions in 42(34.7%) cases and
20(16.5%) cases were numerical aberrations. 121 carriers during 2008 to
2018 and 428 non-carriers in the 2018 were followed up for two years. 55
carriers and 229 non-carriers were subsequent pregnant after diagnosis
by natural conception or intrauterine insemination. The frequency of
carriers to have a health newborn was not significantly different with
non-carriers (72.7% vs. 71.2%, adjusted p=0.968). Conclusion(s): This
study described the majority of carriers were balanced translocations
and chromosome aberrations had a limited influence on live birth rate
from the present data. The results of the study also remind us that
natural conception may be also a good alternative rather than PGD
(Pre-implantation Genetic Diagnosis) which is common in many other
reproductive centers for such patients.
Hospital Information System-Based Pharmacovigilance for Elderly Patients: Practices and Results in China
Authors: Zhou Nan , Fang Zhiyuan , Liang Dong , Lian Jiangping , Han Huan , Zhang Peng , Zuo Yan , Li Rui
Published: January 2024

Purpose: Multiple diseases are often found in an elderly person
concurrently, and most are chronic diseases. A wide variety of drugs are
available for treatment but have prominent drugs interaction problems.
Our research and development pharmacovigilance system (PVS) for the
detection of potentially inappropriate medication (PIM) is a novel alert
system which for reducing PIM prescriptions of elderly patients. The aim
of this study was to evaluate the effect of a PVS on PIM prescriptions
for elderly patients in our hospital. Methods: All drugs that pose risks
to elderly patients, risk reasons, grade of recommendation in the
guidelines and consensus were concluded, incorporated and summarised as
the document information of the PVS for elderly patients. It was
imported into the hospital information system (HIS) in batches. The real
time audit of different dimensions will be conducted on the medical
orders issued according to the different contents of the entries in the
PVS. Results: The PVS has been applied into trial operation in the five
geriatric departments of our hospital. The PVS for elderly patients has
provided warnings 3911 times on outpatient and 3999 times on inpatient
for six months. PVS had great influence on the usage of sedatives and
hypnotics (estazolam, clonazepam, diazepam, zolpidem, alprazolam). The
overall prevalence of PIM was significantly decreased during the 6
months. In January, warning percentage was 16.15% on outpatients and
30.59% on intpatients. However, warning percentage was 6.70% on
outpatients and 16.06% on intpatients in June. Conclusions: The
combination of the information platform and PIM could provide deeper
technical support for the safety of medical behaviour and individualised
treatment of patients.
Biological Evolution Reflected from Sediment Ancient DNA by the High-throughput Sequencing
Authors: Li Xinlu , Liu Xinsheng , Dong Zhande , Qiao Zian , Gao Bo , Li Wannan , Han Lu
Published: January 2024

Sediment ancient DNA can trace the climate and environmental changes of
its surrounding areas, which is helpful to explore the relationship
among the biological diversities, climate changes and human activities.
Here, metabarcoding and metagenomics of high-throughput sequencing were
summarized for better understanding the different methods for sediment
ancient DNA study. Compared with the metabarcoding and metagenomics, it
was found that different methods were selected for the different
research purposes. The research samples of ancient DNA were changed from
single species to multiple species, according to the development of the
sequencing technology. Now, High-throughput sequencing technology has
played an important role in ancient DNA study, which is widely used for
the studies of sediment ancient DNA. In this review, we summarized the
research progress in different stages and some problems of sediment
ancient DNA. The significance of sediment ancient DNA were shown in this
work. In conclusion, both metabarcoding and metagenomics have their own
advantages and disadvantages, even both of them were belonged to the
high-throughput sequencing technology. So, The significance of this
study is to summarize and compare the research methods of sediment
ancient DNA under the high-throughput sequencing, it had guiding
significance for future research.