bluuebunny/crossref_metadata_2025 huggingface dataset:
Ultrasound-Guided Genicular Nerve Thermal Radiofrequency Ablation for Chronic Knee Pain
Authors: Wong Joshua , Bremer Nicholas , Weyker Paul D. , Webb Christopher A. J.
Published: October 2016

Osteoarthritis (OA) of the knee is one of the most common joint diseases affecting adults in the United States. For elderly patients with multiple medical comorbidities who do not wish to undergo total knee arthroplasty (TKA), lifestyle modification, pharmacologic management, and injections are the mainstay of therapy. Previously, pain management interventions were limited to intra-articular joint injections and viscosupplementation with hyaluronic acid. Fluoroscopic-guided techniques for radiofrequency ablation (RFA) of the genicular nerves have been previously described and a recent cadaveric study suggests that ultrasound-guided genicular nerve blocks can be performed accurately. We performed an ultrasound-guided radiofrequency ablation of the genicular nerves in 88-year-old woman who had deferred surgical management given her age. Following successful ultrasound guided diagnostic genicular nerve blocks, she proceeded to RFA using the same ultrasound guided technique. The procedure resulted in significant pain relief and improvement in overall function for greater than 6 months. The use of ultrasound provides a relatively rapid and noninvasive method to directly visualize genicular nerves and surrounding vasculature. Our case suggests that, for genicular nerve blockade and RFA, ultrasound may be a useful alternative to fluoroscopy. Not only did the procedure result in significant pain relief that has persisted for greater than 6 months but also more importantly her function status and quality of life were improved.
Design of Connectivity Preserving Flocking Using Control Lyapunov Function
Authors: Erfianto Bayu , Bambang Riyanto T. , Hindersah Hilwadi , Muchtadi-Alamsyah Intan
Published: October 2016

This paper investigates cooperative flocking control design with connectivity preserving mechanism. During flocking, interagent distance is measured to determine communication topology of the flocks. Then, cooperative flocking motion is built based on cooperative artificial potential field with connectivity preserving mechanism to achieve the common flocking objective. The flocking control input is then obtained by deriving cooperative artificial potential field using control Lyapunov function. As a result, we prove that our flocking protocol establishes group stabilization and the communication topology of multiagent flocking is always connected.
Comparison and Prognostic Analysis of Adjuvant Radiotherapy versus Salvage Radiotherapy for Treatment of Radically Resected Locally Advanced Esophageal Squamous Cell Carcinoma
Authors: Xu Xin , Xie Hua-Ying , Zhou Di , Huang Ren-Hua , Bai Yong-Rui , Yuan Jun , Ye Ming
Published: October 2016

Objective. To compare adjuvant radiotherapy and salvage radiotherapy after radical resection for treatment of esophageal squamous cell carcinoma (ESCC).Methods. Data from 155 patients with locally advanced ESCC who underwent radical resection and received postoperative radiotherapy from 2005 to 2011 were reviewed. Seventy-nine patients received adjuvant radiotherapy and 76 received salvage radiotherapy after locoregional recurrence.Results. The median disease-free survival (DFS) and overall survival (OS) were significantly higher in the adjuvant radiotherapy group than the salvage radiotherapy group (DFS 25.73 months versus 10.73 months,P<0.001; OS 33.33 months versus 26.22 months,P=0.006). The independent prognostic factors for DFS were performance status (PS) before radiotherapy and pathological stage in the adjuvant radiotherapy group, compared with lymph node metastasis, tumor location, and adjuvant chemotherapy in the salvage radiotherapy group. The independent prognostic factors for OS were age and PS in both groups. No differences in median DFS and OS between the groups were observed in patients aged > 65 years or with PS ≥ 2.Conclusion. Compared to salvage radiotherapy, postoperative adjuvant radiotherapy can prolong DFS and OS for patients with radically resected local advanced ESCC but cannot improve survival for patients aged > 65 years or with PS ≥ 2.
Triflumuron Effects on the Physiology and Reproduction ofRhodnius prolixusAdult Females
Authors: Henriques Bianca Santos , Genta Fernando Ariel , Mello Cícero Brasileiro , Silva Lucas Rangel , Codogno Thaís Franco , Oliveira Alyne F. R. , Marinho Lourena Pinheiro , Valle Denise , Lima José Bento Pereira , Feder Denise , Gonzalez Marcelo Salabert , Azambuja Patricia
Published: October 2016

We evaluated the efficacy of the growth regulator triflumuron (TFM) in inducing mortality and disrupting both oviposition and egg hatching inRhodnius prolixusadult females. TFM was administered via feeding, topically or by continuous contact with impregnated surfaces. Feeding resulted in mild biological effects compared with topical and impregnated surfaces. One day after treatment, the highest mortality levels were observed with topical surface and 30 days later both topical and impregnated surfaces induced higher mortalities than feeding. Oral treatment inhibited oviposition even at lower doses, and hatching of eggs deposited by treated females was similarly affected by the three delivery modes. Topical treatment of eggs deposited by nontreated females significantly reduced hatching. However, treatment per contact of eggs oviposited by untreated females did not disrupt eclosion. Additionally, oral treatment increased the number of immature oocytes per female, and topical treatment reduced the mean size of oocytes. TFM also affected carcass chitin content, diuresis, and innate immunity of treated insects. These results suggest that TFM acts as a potent growth inhibitor ofR. prolixusadult females and has the potential to be used in integrated vector control programs against hematophagous triatomine species.
Diffusion Strategies for Distributed Kalman Filter with Dynamic Topologies in Virtualized Sensor Networks
Authors: Yang Shujie , Huang Tao , Guan Jianfeng , Xiong Yongping , Wang Mu
Published: October 2016

Network virtualization has become pervasive and is used in many applications. Through the combination of network virtualization and wireless sensor networks, it can greatly improve the multiple applications of traditional wireless sensor networks. However, because of the dynamic reconfiguration of topologies in the physical layer of virtualized sensor networks (VSNs), it requires a mechanism to guarantee the accuracy of estimate values by sensors. In this paper, we focus on the distributed Kalman filter algorithm with dynamic topologies to support this requirement. As one strategy of distributed Kalman filter algorithms, diffusion Kalman filter algorithm has a better performance on the state estimation. However, the existing diffusion Kalman filter algorithms all focus on the fixed topologies. Considering the dynamic topologies in the physical layer of VSNs mentioned above, we present a diffusion Kalman filter algorithm with dynamic topologies (DKFdt). Then, we emphatically derive the theoretical expressions of the mean and mean-square performance. From the expressions, the feasibility of the algorithm is verified. Finally, simulations confirm that the proposed algorithm achieves a greatly improved performance as compared with a noncooperative manner.
Impaired Albumin Uptake and Processing Promote Albuminuria in OVE26 Diabetic Mice
Authors: Long Y. S. , Zheng S. , Kralik P. M. , Benz F. W. , Epstein P. N.
Published: October 2016

The importance of proximal tubules dysfunction to diabetic albuminuria is uncertain. OVE26 mice have the most severe albuminuria of all diabetic mouse models but it is not known if impaired tubule uptake and processing are contributing factors. In the current study fluorescent albumin was used to follow the fate of albumin in OVE26 and normal mice. Compared to normal urine, OVE26 urine contained at least 23 times more intact fluorescent albumin but only 3-fold more 70 kD fluorescent dextran. This indicated that a function other than size selective glomerular sieving contributed to OVE26 albuminuria. Imaging of albumin was similar in normal and diabetic tubules for 3 hrs after injection. However 3 days after injection a subset of OVE26 tubules retained strong albumin fluorescence, which was never observed in normal mice. OVE26 tubules with prolonged retention of injected albumin lost the capacity to take up albumin and there was a significant correlation between tubules unable to eliminate fluorescent albumin and total albuminuria. TUNEL staining revealed a 76-fold increase in cell death in OVE26 tubules that retained fluorescent albumin. These results indicate that failure to process and dispose of internalized albumin leads to impaired albumin uptake, increased albuminuria, and tubule cell apoptosis.
Formulations of Amlodipine: A Review
Authors: Sheraz Muhammad Ali , Ahsan Syed Furqan , Khan Marium Fatima , Ahmed Sofia , Ahmad Iqbal
Published: October 2016

Amlodipine (AD) is a calcium channel blocker that is mainly used in the treatment of hypertension and angina. However, latest findings have revealed that its efficacy is not only limited to the treatment of cardiovascular diseases as it has shown to possess antioxidant activity and plays an important role in apoptosis. Therefore, it is also employed in the treatment of cerebrovascular stroke, neurodegenerative diseases, leukemia, breast cancer, and so forth either alone or in combination with other drugs. AD is a photosensitive drug and requires protection from light. A number of workers have tried to formulate various conventional and nonconventional dosage forms of AD. This review highlights all the formulations that have been developed to achieve maximum stability with the desired therapeutic action for the delivery of AD such as fast dissolving tablets, floating tablets, layered tablets, single-pill combinations, capsules, oral and transdermal films, suspensions, emulsions, mucoadhesive microspheres, gels, transdermal patches, and liposomal formulations.
Stochastic Effects for the Reaction-Duffing Equation with Wick-Type Product
Authors: Choi Jin Hyuk , Lee SeungGwan , Kim Hyunsoo
Published: October 2016

We construct new explicit solutions of the Wick-type stochastic reaction-Duffing equation arising from mathematical physics with the help of the white noise theory and the system technique. Based on these exact solutions, we also discuss the influences of stochastic effects for dynamical behaviors according to functionsh1(t),h2(t), and Brownian motionB(t)which are the solitary wave group velocities.
Changes of Nitric Oxide and Peroxynitrite Serum Levels during Drug Therapy in Patients with Obsessive-Compulsive Disorder
Authors: Kouti Leila , Sayyah Mehdi , Mosallanezhad Parisa , Kooti Sara , Aghakoochakzadeh Maryam , Eslami Kaveh
Published: October 2016

Objectives. Some studies have shown that increased nitric oxide (NO) concentrations may be associated with obsessive-compulsive disorder (OCD). In a few animal researches, enhanced synthesis of NO had reversed the effect of selective serotonin reuptake inhibitors (SSRIs). The present study tries to find the effect of treatment with SSRIs on NO serum levels and its product peroxynitrite.Patients and Methods. Patients diagnosed with OCD who are candidates of receiving SSRIs entered this study. Two blood samples were taken from subjects, prior to drug therapy and after the patients had shown some improvements due to their regimen. Serum NO and peroxynitrite levels were measured and their correlation with SSRI use was assessed.Results. 31 patients completed this study. Mean concentrations of NO and peroxynitrite prior to drug therapy were28.63±16.9and5.73±2.5 μmol/L, respectively. These values were 18.87±7.55and2.15±0.94 μmol/L at the second blood test. Withpvalues < 0.05, these differences were considered significant.Conclusion. Patients, who showed improvement of OCD symptoms after a mean duration of SSRI monotherapy of3.531±0.64months, had lower values of NO and peroxynitrite in their sera compared to their levels prior to therapy. Such results can be helpful in finding a predictive factor of response to therapy and augmentation therapy with future drugs that target NO synthesis.
Screening for Subtelomeric Rearrangements in Thai Patients with Intellectual Disabilities Using FISH and Review of Literature on Subtelomeric FISH in 15,591 Cases with Intellectual Disabilities
Authors: Charalsawadi Chariyawan , Khayman Jariya , Praphanphoj Verayuth , Limprasert Pornprot
Published: October 2016

We utilized fluorescence in situ hybridization (FISH) to screen for subtelomeric rearrangements in 82 Thai patients with unexplained intellectual disability (ID) and detected subtelomeric rearrangements in 5 patients. Here, we reported on a patient with der(20)t(X;20)(p22.3;q13.3) and a patient with der(3)t(X;3)(p22.3;p26.3). These rearrangements have never been described elsewhere. We also reported on a patient with der(10)t(7;10)(p22.3;q26.3), of which the same rearrangement had been reported in one literature. Well-recognized syndromes were detected in two separated patients, including 4p deletion syndrome and 1p36 deletion syndrome. All patients with subtelomeric rearrangements had both ID and multiple congenital anomalies (MCA) and/or dysmorphic features (DF), except the one with der(20)t(X;20), who had ID alone. By using FISH, the detection rate of subtelomeric rearrangements in patients with both ID and MCA/DF was 8.5%, compared to 2.9% of patients with only ID. Literature review found 28 studies on the detection of subtelomeric rearrangements by FISH in patients with ID. Combining data from these studies and our study, 15,591 patients were examined and 473 patients with subtelomeric rearrangements were determined. The frequency of subtelomeric rearrangements detected by FISH in patients with ID was 3%. Terminal deletions were found in 47.7%, while unbalanced derivative chromosomes were found in 47.9% of the rearrangements.
Dynamical Analysis and Simulation Validation of Incompletely Restrained Cable-Suspended Swinging System Driven by Two Cables
Authors: Wang Naige , Cao Guohua , Zhu Zhencai , Peng Weihong
Published: October 2016

The flexibility of the suspension multicables and driven length difference between two cables cause the translation and rotation of the platform in the incompletely restrained cable-suspended system driven by two cables (IRCSWs2), which are theoretically investigated in this paper. The suspension cables are spatially discretized using the assumed modes method (AMM) and the equations of motion are derived from Lagrange equations of the first kind. Considering all the geometric matching conditions are approximately linear with external actuator, the differential algebraic equations (DAEs) are transformed to a system of ordinary differential equations (ODEs). Using linear boundary conditions of the suspension cable, the current method can obtain not only the accurate longitudinal displacements of cable and posture of the platform, but also the tension between the platform and cables, and the current method is verified by ADAMS simulation.
A Review on Mechanical and Hydraulic System Modeling of Excavator Manipulator System
Authors: Xu Jiaqi , Yoon Hwan-Sik
Published: October 2016

A recent trend in the development of off-highway construction equipment, such as excavators, is to use a system model for model-based system design in a virtual environment. Also, control system design for advanced excavation systems, such as automatic excavators and hybrid excavators, requires system models in order to design and simulate the control systems. Therefore, modeling of an excavator is an important first step toward the development of advanced excavators. This paper reviews results of recent studies on the modeling of mechanical and hydraulic subsystems for the simulation, design, and control development of excavator systems. Kinematic and dynamic modeling efforts are reviewed first. Then, various approaches in the hydraulic system modeling are presented.
A Kinect-Based Physiotherapy and Assessment Platform for Parkinson’s Disease Patients
Authors: Pachoulakis Ioannis , Xilourgos Nikolaos , Papadopoulos Nikolaos , Analyti Anastasia
Published: October 2016

We report on a Kinect-based, augmented reality, real-time physiotherapy platform tailored to Parkinson’s disease (PD) patients. The platform employs a Kinect sensor to extract real-time 3D skeletal data (joint information) from a patient facing the sensor (at 30 frames per second). In addition, a small collection of exercises practiced in traditional physiotherapy for PD patients has been implemented in the Unity 3D game engine. Each exercise employs linear or circular movement patterns and poses very light-weight processing demands on real-time computations. During an exercise, trainer instruction demonstrates correct execution and Kinect-provided 3D joint data are fed to the game engine and compared to exercise-specific control routines to assess proper posture and body control in real time. When an exercise is complete, performance metrics appropriate for that exercise are computed and displayed on screen to enable the attending physiotherapist to fine-tune the exercise to the abilities/needs of an individual patient as well as to provide performance feedback to the patient. The platform can operate in a physiotherapist’s office and, following appropriate validation, in a home environment. Finally, exercises can be parameterized meaningfully, depending on the intended purpose (motor assessment versus plain exercise at home).
Nonodontogenic Cervical Necrotizing Fasciitis Caused by Sialadenitis
Authors: Yenigun Alper , Veyseller Bayram , Vural Omer , Ozturan Orhan
Published: October 2016

Necrotizing fasciitis is a rapidly progressive infectious disease of the soft tissue with high mortality and morbidity rates. Necrotizing fasciitis is occasionally located in the head and neck region and develops after odontogenic infections. Factors affecting treatment success rates are early diagnosis, appropriate antibiotic treatment, and surgical debridement. We present a necrotizing fasciitis case located in the neck region that developed after sialoadenitis. It is important to emphasize that necrotizing fasciitis to be seen in the neck region is very rare. Nonodontogenic necrotizing fasciitis is even more rare.
Generatingq-Commutator Identities and theq-BCH Formula
Authors: Bonfiglioli Andrea , Katriel Jacob
Published: October 2016

Motivated by the physical applications ofq-calculus and ofq-deformations, the aim of this paper is twofold. Firstly, we prove theq-deformed analogue of the celebrated theorem by Baker, Campbell, and Hausdorff for the product of two exponentials. We deal with theq-exponential functionexpq⁡(x)=∑n=0∞(xn/[n]q!), where[n]q=1+q+⋯+qn-1denotes, as usual, thenthq-integer. We prove that ifxandyare any noncommuting indeterminates, thenexpq⁡(x)expq⁡(y)=expq⁡(x+y+∑n=2∞Qn(x,y)), whereQn(x,y)is a sum of iteratedq-commutators ofxandy(on the right and on the left, possibly), where theq-commutator[y,x]q≔yx-qxyhas always the innermost position. When[y,x]q=0, this expansion is consistent with the known result by Schützenberger-Cigler:expq⁡(x)expq⁡(y)=expq⁡(x+y). Our result improves and clarifies some existing results in the literature. Secondly, we provide an algorithmic procedure for obtaining identities between iteratedq-commutators (of any length) ofxandy. These results can be used to obtain simplified presentation for the summands of theq-deformed Baker-Campbell-Hausdorff Formula.
Proteomic Approaches to Biomarker Discovery in Cutaneous T-Cell Lymphoma
Authors: Ion Alexandra , Popa Iris Maria , Papagheorghe Laura Maria Lucia , Lisievici Cristina , Lupu Mihai , Voiculescu Vlad , Caruntu Constantin , Boda Daniel
Published: October 2016

Cutaneous T-cell lymphoma (CTCL) is the most frequently encountered type of skin lymphoma in humans. CTCL encompasses multiple variants, but the most common types are mycosis fungoides (MF) and Sezary syndrome (SS). While most cases of MF run a mild course over a period of many years, other subtypes of CTCL are very aggressive. The rapidly expanding fields of proteomics and genomics have not only helped increase knowledge concerning the carcinogenesis and tumor biology of CTCL but also led to the discovery of novel markers for targeted therapy. Although multiple biomarkers linked to CTCL have been known for a relatively long time (e.g., CD25, CD45, CD45RA, and CD45R0), compared to other cancers (lymphoma, melanoma, colon carcinoma, head and neck cancer, renal cancer, and cutaneous B-cell lymphoma), information about the antigenicity of CTCL remains relatively limited and no dependable protein marker for CTCL has been discovered. Considering the aggressive nature of some types of CTCL, it is necessary to identify circulating molecules that can help in the early diagnosis, differentiation from inflammatory skin diseases (psoriasis, nummular eczema), and aid in predicting the prognosis and evolution of this pathology. This review aims to bring together some of the information concerning protein markers linked to CTCL, in an effort to further the understanding of the convolute processes involved in this complex pathology.
Radiation Effects in Flow through Porous Medium over a Rotating Disk with Variable Fluid Properties
Authors: Jain Shalini , Bohra Shweta
Published: October 2016

The present study investigates the radiation effects in flow through porous medium over a permeable rotating disk with velocity slip and temperature jump. Fluid properties density(ρ), viscosity(μ), and thermal conductivity(κ)are taken to be dependent on temperature. Particular case considering these fluid properties’ constant is also discussed. The governing partial differential equations are converted into nonlinear normal differential equation using similarity alterations. Transformed system of equations is solved numerically by using Runge-Kutta method with shooting technique. Effects of various parameters such as porosity parameterK, suction parameterWs, rotational Reynolds number Re, Knudsen number Kn, Prandtl number Pr, radiation parameterN, and relative temperature difference parameterεon velocity profiles along radial, tangential, and axial direction and temperature distribution are investigated for both variable fluid properties and constant fluid properties. Results obtained are analyzed and depicted through graphs and table.
Clusters of Galaxies in a Weyl Geometric Approach to Gravity
Authors: Scholz Erhard
Published: October 2016

A model for the dark halos of galaxy clusters, based on the Weyl geometric scalar tensor theory of gravity (WST) with a MOND-like approximation, is proposed. It is uniquely determined by the baryonic mass distribution of hot gas and stars. A first heuristic check against empirical data for 19 clusters (2 of which are outliers), taken from the literature, shows encouraging results. Modulo a caveat resulting from different background theories (Einstein gravity plus ΛCDM versus WST), the total mass for 15 of the outlier reduced ensemble of 17 clusters seems to be predicted correctly (in the sense of overlapping 1σ error intervals).
Electromagnetic Stirring versus ECAP: Morphological Comparison of Al-Si-Cu Alloys to Make the Microstructural Refinement for Use in SSM Processing
Authors: Torres Luis Vanderlei , Torres Luis Fernando , Zoqui Eugênio José
Published: October 2016

This work evaluates the morphological evolution at the semisolid state of the Al-4.0wt%Si-2.5wt%Cu alloy produced by direct chill casting under electromagnetic stirring (EMS) and by one equal channel angular pressing (ECAP) pass. The ECAP emerged as a promising technique capable of reduction and homogeneous metals microstructure imposing large deformations occurs in a matrix that contains two channels of the same cross-sectional area and forms an angle of 120°. The materials were submitted to reheating treatment in condition of 60% solid fraction at treatment times of 0, 30, and 90 s. Comparing the two cases, we have the presented ECAP process that had an excellent response to the recovery and recrystallization mechanisms, and refined microstructures ideal for thixoforming were produced. Primary particle sizes of about 45 μm and grain sizes of about 75 μm and a circularity shape factor of more than 0.60 were obtained. The low silicon alloy, Al-4.0wt%Si-2.5wt%Cu, presented excellent refinement when processed via equal channel angular pressing, presenting good morphological stability at the semisolid state, without significant changes in size or shape of the solid particles. This fully globular structure is favourable for thixoforming processes.
Effects of Heat-Treatment on the Microstructure and Wear Resistance of a High-Chromium Cast Iron for Rolls
Authors: Guo Zhi-hong , Xiao Fu-ren , Lu Su-ling , Li Han-yun , Liao Bo
Published: October 2016

The variations of microstructure and mechanical properties of a high-chromium cast iron for rolls were studied from as-cast to the final heat treatments. Results show that the as-cast microstructure of the HCCI consists of M7C3carbide, M23C6carbide, martensite matrix, and retained austenite. The large dendritic M7C3carbide surrounds the matrix, and the M23C6carbide is mainly distributed in the matrix. Part of M23C6carbide transforms to M7C3carbide and is dissolved in austenite during austenization at 1020°C. Thus, the amount of M23C6carbide decreases, whereas that of M7C3carbide increases after quenching; the highest hardness is also obtained. After tempering, the martensite transforms to a tempered martensite, and some carbide precipitates in the martensite matrix. The hardness also changes from HRC62.1, which corresponds to quenching, to HRC55.2 and HRC56.3, which correspond to once and twice tempering, respectively. However, tempering could improve the impact toughness and wear resistance of the HCCI.
Chronic Prostatitis Affects Male Reproductive Health and Is Associated with Systemic and Local Epigenetic Inactivation of C-X-C Motif Chemokine 12 Receptor C-X-C Chemokine Receptor Type 4
Authors: Schagdarsurengin Undraga , Teuchert Lisa M. , Hagenkötter Christina , Nesheim Nils , Dansranjavin Temuujin , Schuppe Hans-Christian , Gies Sabrina , Pilatz Adrian , Weidner Wolfgang , Wagenlehner Florian M.E.
Published: October 2016

Background/Aims/Objectives: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) has detrimental effects on the quality of life including the aspect of sexual dysfunction. The aim of the study was to identify if there was an adverse effect on the male genital compartment and if there are systemic or compartment-specific local signals for epigenetic dysregulation of inflammatory factors in CP/CPPS patients. Methods: One hundred five NIH IIIb CP/CPPS patients and 41 healthy men were recruited and underwent investigations of urines, semen and blood. Promoter methylation and expression of the chemokine C-X-C motif chemokine 12 and its receptor C-X-C chemokine receptor type 4 (CXCR4) (involved in the recruitment of mast cells) were analyzed in prostate epithelial cell lines and in healthy volunteers' and patients' blood, ejaculate cell pellets, and separated ejaculate fractions (sperm and seminal somatic cells). Results: Independently from age, CP/CPPS NIH IIIb was associated with significant impairment of sperm motility, morphology and semen pH (p < 0.001). Patients older than 33 years showed significantly increased seminal interleukin-8 and serum prostate specific antigen values. In patients, the CXCR4 mRNA-expression was significantly decreased in whole blood and ejaculate cell pellets due to promoter hypermethylation. Analyses on separated fractions of sperm and seminal somatic cells revealed that sperm DNA was unaffected, whereas somatic cell DNA was differentially methylated. Conclusions: NIH IIIb CP/CPPS has negative effects on surrogate parameters of male fertility and is associated significantly with systemic and local epigenetic inactivation of CXCR4.
Epidermal Growth Factor Receptor Silencing Blunts the Slow Force Response to Myocardial Stretch
Authors: Brea María S. , Díaz Romina G. , Escudero Daiana S. , Caldiz Claudia I. , Portiansky Enrique L. , Morgan Patricio E. , Pérez Néstor G.
Published: October 2016

Background

Myocardial stretch increases force biphasically: the Frank‐Starling mechanism followed by the slow force response (SFR). Based on pharmacological strategies, we proposed that epidermal growth factor (EGF) receptor (EGFR or ErbB1) activation is crucial for SFR development. Pharmacological inhibitors could block ErbB4, a member of the ErbB family present in the adult heart. We aimed to specifically test the role of EGFR activation after stretch, with an interference RNA incorporated into a lentiviral vector (small hairpin RNA [shRNA]‐EGFR).

Methods and Results

Silencing capability of p‐shEGFR was assessed in EGFR‐GFP transiently transfected HEK293T cells. Four weeks after lentivirus injection into the left ventricular wall of Wistar rats, shRNA‐EGFR–injected hearts showed ≈60% reduction of EGFR protein expression compared with shRNA‐SCR–injected hearts. ErbB2 and ErbB4 expression did not change. The SFR to stretch evaluated in isolated papillary muscles was ≈130% of initial rapid phase in the shRNA‐SCR group, while it was blunted in shRNA‐EGFR–expressing muscles. Angiotensin II (Ang II)‐dependent Na+/H+ exchanger 1 activation was indirectly evaluated by intracellular pH measurements in bicarbonate‐free medium, demonstrating an increase in shRNA‐SCR–injected myocardium, an effect not observed in the silenced group. Ang II‐ or EGF‐triggered reactive oxygen species production was significantly reduced in shRNA‐EGFR–injected hearts compared with that in the shRNA‐SCR group. Chronic lentivirus treatment affected neither the myocardial basal redox state (thiobarbituric acid reactive substances) nor NADPH oxidase activity or expression. Finally, Ang II or EGF triggered a redox‐sensitive pathway, leading to p90RSK activation in shRNA‐SCR‐injected myocardium, an effect that was absent in the shRNA‐EGFR group.

Conclusions

Our results provide evidence that specific EGFR activation after myocardial stretch is a key factor in promoting the redox‐sensitive kinase activation pathway, leading to SFR development.
The Reproduction and Crisis of Capitalism in Venezuela under Chavismo
Authors: Dachevsky Fernando , Kornblihtt Juan
Published: October 2016

The current crisis in Venezuela is sometimes said to have been provoked by the response of imperialism and the local oligarchy to the fundamental changes in economic and political relations fostered during the administrations of Hugo Chávez. A quantitative study using various statistical sources shows that the significant increase in oil rent during the Chávez presidency did not translate into a qualitative transformation in the form of state intervention and that, although social expenditures increased in that period, most of the income that allowed this was obtained through currency overvaluation by inefficient national and foreign capital. The current crisis is, therefore, evidence of the limits of low-productivity state and private capital reproduction due to the decline in oil prices rather than of a conflict between overcoming and reproducing capitalism in an alleged “economic war.”

A veces se dice que la crisis actual en Venezuela ha sido provocada por la respuesta del imperialismo y la oligarquía local a los cambios profundos en las relaciones económicas y políticas promovidos durante la administración de Hugo Chávez. Un estudio cuantitativo en el que se usaron diferentes fuentes estadísticas demuestra que el aumento significativo en la renta del petróleo durante la presidencia de Chávez no se tradujo en una transformación cualitativa de la intervención estatal y que, aunque los gastos sociales aumentaron en ese período, la mayor parte del ingreso que permitió ese aumento se obtuvo por medio de la sobrevaluación de la moneda por parte del ineficiente capital nacional y extranjero. Por lo tanto, la crisis actual es evidencia de los límites de la baja productividad de la reproducción estatal y privada debido a la caída en el precio del petróleo y no un conflicto entre la eliminación y la reproducción del capitalismo en una supuesta “guerra económica.”
Four persistent rural healthcare challenges
Authors: Ford Donald M.
Published: October 2016

Today, 25% of Canadians live in rural and remote parts of Canada. The evidence is that these Canadians do not enjoy the same health status as citizens living in more urban settings. This article explores four persistent healthcare challenges: population demographics, place, professionals, and public participation. By exploring solutions that some rural communities have used to address these challenges, this article aims to provide insights into lessons that have been learned that they may be considered and potentially applied to both rural and urban environments in the interest of better healthcare for all.
Rethinking the financing of healthcare in Canada
Authors: Taylor D. Wayne
Published: October 2016

Canadians need to talk about their healthcare, about who pays for what, when. Lack of money is not the issue; how that money is spent is the issue—what public healthcare is covering and not covering. The same dollar amount can be spent quite differently and more effectively. The 1950s first-dollar, single-payor decision shifted the burden from the individual to government, but a lot has changed since the 1950s. Today Medicare is not universal, comprehensive, reasonably accessible, or portable. With residual constitutional power residing in Ottawa, there is no reason for the fragmentation and inequalities facing Canadians. Sound fiscal policy enables funding at appropriate levels when needed; however, current fiscal policy is not sound. Canada can learn from countries that outperform it in terms of quality, waits, access, outcomes, and value for money. This article provides some fresh thinking about the financing of a system that currently is failing Canadians.
Universal prescription drug coverage in Canada
Authors: Morgan Steven G. , Boothe Katherine
Published: October 2016

Canada’s universal public healthcare system is unique among developed countries insofar as it does not include universal coverage of prescription drugs. Universal, public coverage of prescription drugs has been recommended by major national commissions in Canada dating back to the 1960s. It has not, however, been implemented. In this article, we extend research on the failure of early proposals for universal drug coverage in Canada to explain failures of calls for reform over the past 20 years. We describe the confluence of barriers to reform stemming from Canadian policy institutions, ideas held by federal policy-makers, and electoral incentives for necessary reforms. Though universal “pharmacare” is once again on the policy agenda in Canada, arguably at higher levels of policy discourse than ever before, the frequently recommended option of universal, public coverage of prescription drugs remains unlikely to be implemented without political leadership necessary to overcome these policy barriers.
Bioethics, health technology reassessment, and management
Authors: Soril Lesley J. J. , Clement Fiona M. , Noseworthy Tom W.
Published: October 2016

Health Technology Reassessment (HTR) is an emerging area of health services and policy research that supports optimal management of technologies throughout their lifecycle. As a structured, evidence-based assessment of the clinical, economic, social, and ethical impacts of existing technologies, HTR is a means of achieving optimal use, managed exit, and better value for money from technologies used in healthcare. This has been documented as raising ethical concerns among clinicians who are providing direct patient care, particularly when managed exit may be the goal. This article discusses the ethical considerations relevant to clinicians and HTR, using a principles’ approach to bioethical decision-making.
The ethical case for providing cost-free access to lifesaving HIV medications in Canada
Authors: Kaposy Chris , Greenspan Nicole R. , Marshall Zack , Allison Jill , Marshall Shelley , Kitson Cynthia
Published: October 2016

Antiretroviral therapy for HIV can be expensive if paid for out of pocket. In Canada, there are a variety of federal, provincial, and private prescription drug plans that lower the cost of these lifesaving medications for people living with HIV, and in some cases, these plans result in cost-free access. However, many people living with HIV must contend with high deductibles for their antiretroviral therapies, and many experience difficulty managing the administrative requirements of their drug plans. This article comments on some of the results of a qualitative study into ethical issues in HIV care. Access to antiretrovirals was a theme that emerged in this study. We argue on ethical grounds that provincial drug plans should guarantee cost-free access to antiretroviral therapies for people living with HIV with minimal administrative requirements.
The Tribal Tobacco Education and Policy Initiative: Findings From a Collaborative, Participatory Evaluation
Authors: Scott Sheryl , D’Silva Joanne , Hernandez Carol , Villaluz Nicole Toves , Martinez Jaime , Matter Chris
Published: October 2016

While the reduction in the overall U.S. smoking prevalence has been declared one of the top 10 public health achievements of the past century, the growing disparity in smoking between American Indians and the general population is one of the biggest challenges of the 21st century. Minnesota in particular has very high smoking rates among American Indians (59%). Tribal Nations in Minnesota share a past of attempted cultural genocide and a present of restoring the strength of their cultural teachings, including the prominence of traditional tobacco as a sacred “first medicine.” The Tribal Tobacco Education and Policy initiative works to address this complex and challenging context. This article describes results of a participatory evaluation from 2010 to 2013 in four Minnesota Tribal Nations—three Ojibwe and one Dakota. Tribal Tobacco Education and Policy coordinators used their cultural knowledge to develop community-level strategies, identifying appropriate strategies from best practices on tobacco advocacy, while drawing on the strengths of their own sovereignty and sacred tobacco traditions. Tribal coordinators generated support for policy change by conducting culturally relevant education, engaging tribal members, and nurturing relationships. This approach resulted in norm changes, practices toward restoring traditional tobacco, informal policies, and tribal resolutions to advance smoke-free policies.
Development and Validity of Western University’s On-Road Assessment
Authors: Classen Sherrilene , Krasniuk Sarah , Alvarez Liliana , Monahan Miriam , Morrow Sarah A. , Danter Tim
Published: October 2016

Although used across North America, many on-road studies do not explicitly document the content and metrics of on-road courses and accompanying assessments. This article discusses the development of the University of Western Ontario’s on-road course, and elucidates the validity of its accompanying on-road assessment. We identified main components for developing an on-road course and used measurement theory to establish face, content, and initial construct validity. Five adult volunteer drivers and 30 drivers with multiple sclerosis participated in the study. The road course had face and content validity, representing 100% of roadway components determined through a content validity matrix and index. The known-groups method showed that debilitated drivers (vs. not debilitated), made more driving errors ( W = 463.50, p = .03), and failed the on-road course, indicating preliminary construct validity of the on-road assessment. This research guides and empirically supports a process for developing a road course and its assessment.
Trehalose preincubation increases mesenchymal (CD271+) stem cells post-cryopreservation viability
Authors: Kusuma Indra , Hadi Restu S. , Kiranadi Bambang , Boediono Arief
Published: October 2016

Background: Dimethyl sulfoxide (Me2SO) is a common cryoprotective agent widely used in cell preservation system. Me2SO is currently known to cause epigenetic changes which are  critical in stem cells development and cellular differentiation. Therefore, it is imperative to develop cryopreservation techniques that protect cellular functions and avert Me2SO adverse effect. Trehalose was able to protect organism in extreme condition such as dehydration and cold. This study aimed to verify the protective effect of trehalose preincubation procedure in cryopreservation.Methods: The study was conducted using experimental design. Thawed mesenchymal (CD271+) stem cells from YARSI biorepository were used for the experiment. Trehalose preincubation was performed for 1 hour, internalized trehalose was confirmed by FTIR-ATR measurement. Three groups consisted of (1) cryopreserved without trehalose preincubation, (2) cryopreserved with trehalose preincubation, and (3) did not undergo cryopreservation were evaluated after 24 hours in LN2 for viability in culture. The absorbance from each group was measured at 450 nm. The analysis performed using paired student t test.Results: Viability of thawed mesenchymal (CD271+) stem cells that undergo trehalose preincubation prior cryopreservation was significantly higher (p<0.05) compared to group without trehalose preincubation. Higher viability observed between group with trehalose preincubation compared with controlled group suggests protection to trypsinization. Mesenchymal (CD271+) stem cells incubated for 1 hour in 100 mM trehalose supplemented medium  results in 15%  trehalose loading efficiency.Conclusion: These findings confirm the protective effect of trehalose preincubation in cryopreservation. Future research should be directed to elucidate the trehalose internalization mechanism and eventually the protective mechanism of trehalose in mammalian cell cryopreservation.
Altered expressions of endothelial junction protein of placental capillaries in premature infants with intraventricular hemorrhage
Authors: Ekawati Maria , Mujihartini Ninik , Jusuf Ahmad A. , Dharmasetiawani Nani , Jusman Sri W.A. , Sadikin Mohamad
Published: October 2016

Background: Placental hypoxia may lead to oxidative stress, which inflicts damage to capillary protein junction. The aim of this study was to evaluate altered expression of endothelial junction protein of capillaries in hypoxia condition and to observe its correlation with the incidence of  intraventricular hemorrhage in premature infants.Methods: A cross-sectional study was conducted by using placental tissues of premature infants as amodel of capillary integrity (29 hypoxic and 29 non-hypoxic). Hypoxia inducible factor (HIF)-1α was measured to define placental tissue response to hypoxia; malondialdehyde (MDA) and glutathione (GSH) served as markers of oxidative stress. The expressions of junctional proteins, N-cadherin and occludin were analyzed by immunohistochemistry. Intraventricular hemorrhage (IVH) was detected by cranial ultrasound at the third day. Unpaired t test, Mann-Whitney, and Chi-square tests were used to analyze the data.Results: The HIF-1α and MDA levels were slightly, but not significantly, higher in hypoxia group {13.64±8.70 pg/mg protein and 10.31 pmol/mg tissue (ranged 1.92–93.61), respectively}  compared to non- hypoxia group {10.65±5.35 pg/mg protein and 9.77 pmol/mg tissue (ranged 2.42–93.31)}. GSH levels were not different in both groups (38.14 (ranged 9.44–118.91) and  38.47(ranged 16.49–126.76) ng/mg protein, respectively. mRNA expression of N-cadherin (0.13) and occludin (0.096) were significantly lower in hypoxia comparedto non-hypoxia group (p=0,001), while protein expression of  N-cadherin (3.4; 75.9; 6.9; 13.8%) and occludin  (20.7; 3.4; 69.0; 3.4; 6.9%)  in hypoxia group was not associated with IVH (p=0.783 and p=0.743).Conclusion: Hypoxia altered expression of endothelial junction protein in placental capillaries, but no association with intraventricular hemorrhage was observed.
Association of ATP-binding cassette sub-family B member 1 gene C3435T polymorphism with neutropenia in breast cancer patients treated with chemotherapy
Authors: Syarifah Siti , Siregar Kamal B. , Siregar Yahwardiah
Published: October 2016

Background: Neutropenia is the most common adverse event of breast cancer chemotherapy which can be life threatening due to opportunistic infection, neutropenic episodes may lead to delay or reduction of drug doses which may compromise treatment outcomes. In this study, we investigated the association of ATP-binding cassette sub-family B member 1 (ABCB1) gene C3435T polymorphism with the grading of neutropenia in breast cancer patients who treated with doxorubicin-taxan.Methods: 72 Indonesian female breast cancer patients from Haji Adam Malik Hospital who had been diagnosed and treated with doxorubicin-taxane regimen were selected for this cohort study. DNA was extracted from peripheral leucocytes and ABCB1 C3435T polymorphism was analyzed with PCR-RFLP. Patient data were collected from patient’s medical record for 3 cycles of chemotherapy. Association between ABCB1 C3435T polymorphism with neutropenia was assessed using Kruskal-Wallis test. Decline of absolute neutrophil count was assessed using Wilcoxon test. Genotype deviation and allele frequencies were also determined by Hardy-Weinberg Equilibrium.Results: The frequencies of ABCB1 C3435T genotype for wildtype (CC), heterozygous (CT) and homozygous mutant (TT) was 22 (30.6%), 38 (52.8%) and 12 (16.7%) respectively. No association were found between ABCB1 C3435T polymorphism and the grading of neutropenia (p>0.05). There was a difference on the average of absolute neutrophil count after the first chemotherapy and after the third chemotherapy (p<0.05). There was no significant deviation of allele and genotype frequency from Hardy-Weinberg Equilibrium.Conclusion: ABCB1 C3435T polymorphism had no association with the grading of neutropenia in breast cancer patients treated with doxorubicin-taxane regimen, however there was a trend of absolute neutrophil count declining during the 3 cycles of chemotherapy.
A rare case of cytomegalovirus papillitis in patient with immunodeficiency
Authors: Devona Dinda A. , Susiyanti Made
Published: October 2016

A 26-year-old male diagnosed with AIDS came with sudden blurred vision and central sco-toma in left eye since 2 weeks before admission. His visual acuity was counting finger at 5 meters with normal IOP and anterior segment. The posterior segment revealed edematous optic nerve covered by exudates and hemorrhages. Due to low CD4+ count and serological test result, we considered a HIV-related opportunistic ocular infection, specifically HSV infection. As visual acuity worsened during treatment with acyclovir, we performed PCR ex-amination from aqueous tap which revealed positive CMV DNA. Unfortunately, the visual acuity had worsened to no light perception before he received any specific anti-CMV agent. CMV papillitis is an unusual presentation of CMV retinitis. PCR examination from aqueous or vitreous tap should be performed while waiting for serological test result, especially in doubtful cases. Therefore, appropriate diagnosis and management can be established early to prevent irreversible visual loss.