bluuebunny/crossref_metadata_2025 huggingface dataset:
Aula invertida o clase magistral innovar o morir
Authors: Saravia-Rojas Miguel Á. , Casas-Chavez Elizabeth
Published: April 2024

Las estrategias para comunicar la información han experimentado muchos cambios, los cuales se han evidenciado en la pandemia. La idea es lograr que se pueda conectar con el estudiante y que la información la reciba de manera clara, oportuna y pertinente, de modo que tendrá los saberes previos para lograr integrar la información de la teoría y la práctica, con el fin de enfrentar con éxito la actividad simulada, así como cuando interactúe con el paciente. En ese contexto, el aula invertida es una estrategia didáctica que brinda videos, podcast, entre otros, para que sean revisados con anticipación, de tal forma que cuando el estudiante realice la actividad presencial o virtual se profundice y se hagan precisiones sobre los conceptos más importantes, usando metodologías activas de participación para lograr el siguiente propósito: El aprendizaje investido busca un cambio en la dinámica del aprendizaje que apunte como eje del mismo al estudiante. El objetivo de la presente contribución docente es compartir las bondades de esta estrategia usada en estudiantes de la carrera de Odontología dentro del marco del curso de Clínica Estomatológica del Adulto I, dictado en 2023 y que pertenece al quinto ciclo de la carrera de Estomatología en la Universidad Peruana Cayetano Heredia.
Premolares con tres conductos radiculares
Authors: Araujo Rosario , Vega Margarita
Published: April 2024

El sistema de conductos radiculares es complejo. En él podemos encontrar piezas dentales como los premolares, cuya anatomía interna es variable. Así pues, en las piezas premolares superiores predominan tres conductos, mientras que en las piezas premolares inferiores se observa un menor porcentaje de incidencia. Hoy en día, el uso de las tomografías computarizadas es indispensable ya que estas nos brindan imágenes tridimensionales que nos ayudan a generar un correcto diagnóstico, garantizar un adecuado procedimiento y lograr el mejor pronóstico favorable para una endodoncia. El propósito del presente artículo de revisión es compendiar información en una búsqueda manual de artículos diferentes de investigación científica de PubMed y Google Académico, donde se describirán las variaciones anatómicas, el diagnóstico y el tratamiento de los dientes premolares con tres conductos.
Terapia fotodinámica, una nueva tendencia en endodoncia para la eliminación del Enterococcus faecalis
Authors: Calderón Augusto José Carlos Martín , Cassana Rojas Luis Rodrigo , Villar Zapata Jean Carlo , Velásquez Huamán Zulema
Published: April 2024

La desinfección se consigue mediante procedimientos como la instrumentación, la irrigación y la medicación intraconducto; sin embargo, estos no son suficientes, ya que diversos estudios han reportado al E. faecalis como uno de los microorganismos persistentes con mayor prevalencia en tratamientos de conductos que no han conseguido la curación de los tejidos periapicales. Se ha tratado de mejorar los protocolos de desinfección incluyendo diferentes herramientas tecnológicas como en el caso de la terapia fotodinámica, la cual utiliza una fuente de luz y sustancias fotosensibilizantes que favorecen el proceso de eliminación de las bacterias remanentes dentro del sistema de conductos radiculares. La presente revisión de literatura científica profundiza en la importancia clínica de la terapia fotodinámica y su efecto en la desinfección e inhibición del E. faecalis dentro del sistema de conductos, lo cual se ha vuelto una pieza clave para el éxito del tratamiento endodóntico.
Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition
Authors: Delaiah Diena , Aswin Ahmad , Susilowati Helen , Wijaya Andi Yasmin , Maulana Firdausy Kurnia , Diyantoro Diyantoro , Rodprasert Watchareewan , Puspitasari Yulianna , Dhamayanti Yeni , Kuncorojakti Suryo
Published: April 2024

Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.
Effect of Gum Arabic (Acacia senegal) on Growth Performance, Carcass Quality and Health of Clarias gariepinus Juveniles
Authors: Falaye Augustine Eyiwunmi , Abah Abel , Sule Shakiru Okanlawon
Published: April 2024

The effect of Gum Arabic (GA) on growth, carcass quality, blood parameters, gut morphometry, and organ histology of African catfish Clarias gariepinus was studied. Five isonitrogenous diets with inclusion levels of GA at 0 g/100 g (GA0; control), 5 g/100 g (GA5), 10 g/100 g (GA10), 15 g/100 g (GA15), and 20 g/100 g (GA20) were formulated in a 40% crude protein diet. Juveniles of C. gariepinus with average weight (7.35±0.48 g/fish) were stocked at 13 fish/25 L of water for 12 weeks in a completely randomized design and fed at 3% body weight daily. Growth indices showed GA10 improved the mean weight gain (69.88±8.89 g) and specific growth rate (2.76±0.14 %/day) while at (GA15); (GA20) growth was depressed. Carcass quality revealed dietary levels were superior to control with GA10 significantly different (p < 0.05) amongst treatments in protein and fat content. Hematological profile showed variations; erythrocyte indices compared to control, while plasma chemistry of aspartate aminotransferase, alkaline phosphatase, and alanine aminotransferase showed no difference (p > 0.05). Gut morphometrics varied with no particular pattern. At inclusion above GA5 the gill, liver, and kidney were affected histologically with no changes in the intestines of all treatments. Diet supplemented with gum arabic at 10 g/100 g improved indices of performance of C. gariepinus juveniles in this study.
Identification of Escherichia coli Multidrug Resistance in Cattle in Abattoirs
Authors: Mustika Yolla Rona , Effendi Mustofa Helmi , Puspitasari Yulianna , Plumeriastuti Hani , Khairullah Aswin Rafif , Kinasih Kurnia Nisa
Published: April 2024

Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this study was done on the detection and identification of multidrug-resistant E. coli in cattle in Surabaya abattoirs. Each cattle rectum swab sample was streaked into EMB agar media and followed by Gram staining. The IMViC test was used to confirm the presence of E. coli. Based on morphological culture features, Gram staining, and biochemical testing, the sample examination results revealed that 41 samples (41%) of the 100 cattle rectal swab samples that were isolated were confirmed to be positive for E. coli. The profile of antibiotic resistance from the results of the E. coli resistance test to antibiotics showed that out of a total of 41 isolates of E. coli, 12 isolates (29.27%) were detected as resistant to 1 class of antibiotics tested, while 2 isolates (4.88%) were resistant to 2 classes of antibiotics, and 3 isolates (7.32%) were confirmed to be multidrug resistance (MDR).
First Report of Aeromonas schubertii Infection in Striped Snakehead Channa striata (Bloch, 1793) Fingerlings in Malaysia
Authors: Tahir Nur Diyana Mohamad , Matori Mohd Fuad , Gan Han Ming
Published: April 2024

Striped snakehead fingerlings Channa striata reared in a concrete pond with size 13.5 m3 (3 m ´ 3 m ´ 1.5 m) in a hatchery farm, in Labu, Negeri Sembilan, Malaysia had more than 70% mortality. The pond contained 1500 fingerlings of 5–7 cm in total length (TL). The purpose of this study was to investigate the high mortality event on the farm as part of a veterinary diagnostics service. Clinical signs observed were skin discoloration, lethargy, and anorexia. Necropsy was performed in 10 fish with gross lesions of 1–2 mm and multiple whitish nodules in the abdominal organs and most notably in the liver, and kidney. Following necropsy, bacteriology samples from the kidney and liver were inoculated onto Tryptic Soy Agar (TSA) and Blood Agar (BA). Pure colonies were observed and were Gram-negative with short rod morphology. The full-length 16S rRNA gene was amplified from the pure colonies followed by sequencing using the Nanopore MinION™ (Oxford Nanopore, UK). The isolated strain was identified as Aeromonas schubertii based on the assembled 16S rRNA sequence showing nucleotide identity of 99.73% (top hit) to A. schubertii (GenBank Accession: NR_037014.2) while only a nucleotide identity of 97.55% was observed against Aeromonas hydrophila (GenBank Accession: NR_119190.1-), respectively. Histopathological examination from the liver, kidney, spleen, and intestine revealed granulomatous nephritis, and degeneration of tubular epithelium, with granulomatous hepatitis, splenitis, and enteritis. The prognosis of this case was grave. No treatment was given as the owner had decided to dispose of all current stock. This is the first A. schubertii infection in C. striata described in Malaysia. The findings of the present study may serve as a reference for similar cases in C. striata in the future so rapid diagnosis and treatment can be made if required.
Detection of Foot and Mouth Disease Virus in Salted Raw Cowhide from Malaysia in Tanjung Priok Port, Indonesia
Authors: Sekarsana Disty Ayu , Basri Chaerul , Lukman Denny Widaya
Published: April 2024

Animal products such as raw salted cowhide are thought to have the potential to transmit the foot and mouth disease (FMD) virus from the infected zone. Indonesia imports raw salted cowhide from Malaysia, so it has the potential to transmit FMD to Indonesia which enters through Tanjung Priok Port. This study aimed to investigate the presence of the FMD virus in raw salted cowhide from Malaysia. The number of samples was collected from each container of raw salted cowhide imported through Tanjung Priok Port during August–December 2022. A total of 21 samples were obtained from 21 bulk containers containing raw salted cowhide. Real time q Polymerase Chain Reaction (RT-qPCR) was used to investigate foot and mouth disease virus in samples. The RT-qPCR screening test on 21 samples reported that salted raw cowhide was free from the FMD virus. Continuous monitoring and surveillance protocols for salted rawhide imported from non-free countries need to be carried out at other points of entry.
Exploring the Relationship Between Growth Hormone Secretagogue Receptor (GHSR) Gene and Body Proportions in Bangkok Chickens: Insights from DNA Sequencing and Hin6I Enzyme-Restricted PCR-RFLP Analysis
Authors: Syaikhullah Gayuh , Hertamawati Rosa Tri , Adhyatma Muhammad
Published: April 2024

The purpose of this study was to determine the diversity of the growth hormone secretagogue receptor (GHSR) gene and also to analyze the association of the GHSR gene with the body proportions of Bangkok chickens. A total of 125 Bangkok chickens were reared, and blood samples were taken. Bangkok chickens were kept in colony cages with ad libitum feeding and drinking. The GHSR gene polymorphism was determined using the PCR-RFLP method. The restriction enzyme used in this study was Hin6I. The data analyzed were indicators of body proportions, which included data on body weight, carcass weight, and commercial cut weight. Single nucleotide polymorphism (SNP) identification using the Molecular Evolutionary Genetics Analysis 7 program with reference number AB095994.1. Allele frequency values, genotype frequencies, and Hardy-Weinberg balance values were also analyzed. The association between the GHSR gene and the traits observed in Bangkok chickens was analyzed using the T-test. The results of the study showed that the GHSR gene in Bangkok chickens had two genotypes, namely TT and CT. The values for Ho and He were 0.224 and 0.198, respectively. The genotype frequencies of TT and CT were 0.776 and 0.224, respectively. The two genotypes were associated with body weight, carcass weight, and commercial weight (p < 0.05). For all measured criteria, the TT genotype showed a higher weight value than the CT genotype. The GHSR gene has the potential to be used as a genetic marker for the selection process on body weight, carcass weight, and commercial weight traits.
Validation of Analytical Method for Aeromonas hydrophila Identification using Analytical Profile Index (API) 20E KIT Method
Authors: Artati Diah , Oman Mohammad , Supriyanto Supriyanto , Sukarta Dede , Robisalmi Adam
Published: April 2024

The high demand for fish consumption has an impact on increasing aquaculture productivity and causes the vulnerability of increasing bacterial populations in aquaculture fields, so more rapid handling is needed. The use of phenotypic KIT methods (API 20E) has been applied as a targeted and efficient identification support in targeting better bacterial identification accuracy but often provides unequal results. Method validation is one of the general requirements for the competence of a laboratory evaluation: to provide coherent, interpretable, and accurate results with known uncertainties. The purpose of this study was to validate the API 20E KIT method for the identification of A. hydrophila. The conventional method used as a reference is SNI 7303.1:2015. The validation parameters consisted of determining the limit of detection, sensitivity, and specificity tests, as well as the positive predictive value and negative predictive value. The results showed that the limit of the detection value of the API 20E KIT was at a concentration of 100 CFU/mL with an ID of 99.00%. The sensitivity and specificity values in the positive and negative target samples were 100% with a positive predictive value and a negative predictive value of 0%, respectively. In conclusion, the API 20E KIT method as an alternative test method or rapid test was proven valid for identifying A. hydrophila by the test results using the reference method.
Early diagnosis and management of inseparable conjoint twins. A low-middle-income country experience
Authors: Aditiawarman
Published: April 2024

HIGHLIGHTS

Conjoined twins have captivated mankind for centuries due to the rarity of this birth type; nonetheless, physicians have constantly encountered difficulties in dealing with conjoined twins.
Early diagnosis of conjoined twins during prenatal examination is critical for ascertaining the prognosis of the fetus, guiding parental counseling over appropriate courses of action, and potentially enabling the termination of the pregnancy.
First-trimester ultrasonography and MRI are complementary modalities in determining the diagnosis and prognosis of conjoined twins in early pregnancy.

 
ABSTRACT
Objectives: To discuss the crucial early diagnosis of conjoined twins to determine the type and prognosis.
Case Report: A 27-year-old woman was referred to the type A referral hospital with suspicion of congenital abnormalities at 17 weeks of pregnancy. Ultrasound results showed intrauterine monochorionic monoamniotic twins with babies fused on their heads to the urogenital part. The MRI showed a craniopagus, suspected meningomyelocele, and severe bilateral hydronephrosis in the second baby. Due to non-separable cases and a bad prognosis for the fetus, the pregnancy was terminated using misoprostol induction and inserting a balloon catheter. The fetus is born weighing 400 g and 20 cm in length. Conjoined twins obtained the rostral type: a fused head with two faces, a fused thorax until the lower abdomen with one umbilicus, and two pairs of hands and feet. The diagnosis of conjoint twins becomes a problem in early pregnancy, mostly in developing countries. Early diagnosis of conjoined twins during prenatal examination is critical for ascertaining the prognosis of the fetus, guiding parental counseling over appropriate courses of action, and potentially enabling the termination of the pregnancy to prevent maternal stress and complications.
Conclusion: Conjoined twins should be identified as soon as feasible to establish the best course of management for both mother and fetus. Ultrasonography and MRI are modalities for determining the diagnosis and prognosis of conjoined twins.
Agent Banking, Mobile Money Operation and Financial Inclusion in Nigeria: Supply Side Perspective
Authors: Quadri Yusuf , Ayorinde Olutimi Akinwumi  , Kudirat Mopelola Malik-Abdulmajeed  , Ifedolapo Oluwasolape Omotosho
Published: April 2024

Background: The inability to achieve optimum financial inclusion in Nigeria has necessitated the review of various policies and instruments meant to reduce the level of financial exclusion.
Objective: Hence this study investigates the impact of agent banking and mobile money operation on financial inclusion in Nigeria, focusing on the supply side.
Method: Descriptive research design was adopted and secondary data ranging from 2013 to 2021 were obtained from the World Bank Global Financial database and e-payment statistics of the Central Bank of Nigeria. Ordinary least squares repression was used to analyse the data.
Results: Findings revealed that at 5% significance level, point-of-sale and mobile money operations have a positive impact on financial inclusion while web/internet banking plays a limited role in achieving financial inclusion.
Conclusion: The study concluded that both agent banking and mobile money operations impact on financial inclusion in Nigeria; the study, therefore, recommends that more off-site automated teller machine and licensed agents should be encouraged to cater for the rural residents and ultimately improve financial inclusion.
Keywords: Agent Banking, Financial inclusion, Mobile Money Operation
Factors Affecting Employee Performance in Manufacturing Companies: Empirical Studies in Batam City
Authors: Siagian Chelika , Dr. Muhammad Donal Mon
Published: April 2024

Background: The progress of a company is greatly influenced on the performance of its employees working in the company. In this case, the performance of each employee is influenced by several factors. Self-efficacy, organizational support, and good management skill are the aspects which define employee performance’s quality.
Objective: The current field study conducted by staffing researchers in Batam City, shows that employee performance is a very important aspect for increasing productivity and business progress. Employees who can demonstrate knowledge, technical skills, creativity, and organizational experience are very important since they are seen as productive assets which can improve company’s performance as well as company's reputation.
Method: This study was conducted on 270 employees in manufacturing companies in Panbil Area, Batam, Indonesia. This study used Smart PLS 3.0 with multiple linear regression to examine the factors that affect employee performance.
Results: This study found that the results of motivation; transformational leadership; organizational culture; work discipline; job performance; and employee performance have a significant influence on employee performance. The five independent variables have a significant effect on employee performance simultaneously.
Conclusion: Based on the results of this research study, the researcher found that every company will always develop employee performance to achieve company goals. Human resource management needs to apply effective leadership and in accordance with the circumstances in the company. Not only leadership, work environment conditions and motivation, suitable job description will affect employee performance. Working environment conditions that can motivate employees to work will certainly develop employee performance.
Keywords: motivation; transformational leadership; organizational culture; work discipline; job performance; employee performance
Activity-Based Costing Implementation for Capturing the Complexity of Manufacturing Process: The Case of CV XYZ
Authors: Firmandani Wahyu , Arizendy Dewi Fortuna  , Mochammad Nurul
Published: April 2024

Background: In manufacturing companies, determining the cost of goods manufactured is more complex than in service and trading companies, considering that the production cost structure consists of direct raw material costs, direct labor costs, and factory overhead. In identifying the imposition of three components of production costs, the most difficult component to trace is factory overhead because, in determining factory overhead, various approaches and assumptions must be chosen as cost drivers, so it needs the right approach and assumptions for CV XYZ to achieve company performance.
Objective: This study seeks to analyze the intricacies of factory overhead calculations under activity-based costing in comparison to the traditional plantwide rate approach used by CV XYZ, which utilizes production units as cost drivers.
Method: The method used in this research is a case study on CV XYZ with interviews and documentation as data collection techniques. Interviews were conducted with accounting staff and heads of accounting departments through unstructured interviews. Documentation is carried out based on 2020 financial information.
Results: The results of the analysis explain that the calculation of factory overhead applied, production costs, cost of goods manufactured (COGM), and cost of goods sold (COGS) calculated using the plantwide rate approach (production units as cost drivers) shows undercosts when compared to the activity-based costing system, so that the recognized profit is greater than it should be. The implications of undercosts cause information on the income statement to be unreliable, considering that the company has a variety of products and activities related to the production process
Conclusion: The activity-based costing system uses more than one cost driver; thus, the activity-based costing system is a more accurate method to be applied by CV XYZ, which has product diversification.
Keywords: Factory overhead applied, plantwide rate, activity-based costing system, profit and loss.
The Mediating Role of Brand Trust on the Effect of Brand Ambassador to Purchase Decision
Authors: Then Lydia , Isnaini Nuzula Agustin
Published: April 2024

Background: Beauty products from South Korea, such as skincare and makeup, are often considered as having higher quality and providing better results than local beauty products. With technological advances that continue to develop, obtaining skincare product is currently easier and more accessible to young people.
Objective: This research aims to examine the factors that influence purchasing decisions in regards to brand trust as a mediator in Korean skincare products.
Method: The sampling employed a purposive sampling method to a population in Batam City who aged over 17 years old and used skincare products made in South Korea. This research used Partial Least Square Structural Equation Modeling (PLS-SEM) for hypothesis testing.
Results: The research results indicate that brand trust plays an important role in mediating the relationship between brand ambassadors, brand awareness, and price on purchasing decisions. Brand image, brand awareness, and price directly and indirectly influence purchasing decisions. Meanwhile, brand ambassadors only indirectly influence purchasing decisions through brand trust.
Keywords: Purchase Decision; Brand Trust; Brand Ambassador; Brand Awareness; Korean Skincare
‎ Affiliate Marketer a Decent Work for Generation Z in Indonesia?‎
Authors: Nugraha Arlan , Aulia Pratiwi Monica , Muharni Ardenis Dwi , Amany Nurfatimah , Ahmad Sarah Sabir , Sukmawati Anggraini
Published: April 2024

Background: Affiliate marketers have grown significantly as they serve as promoters, connecting e-commerce platforms with social media audiences and providing profitable income opportunities.
Objective: This study assesses the alignment between affiliate marketing practices in Indonesia and the decent work indicator as defined by the International Labor Organization (ILO). Additionally, the study aims to compare respondents’ income before and after engaging in affiliate marketing.
Method: This study employed random sampling to select a sample of 25 affiliate marketers from the Jabodetabek region in Indonesia. Participants responded to 23 questions developed based on the 11 Decent Work Indicators established by the ILO, utilizing a 1 to 4 ordinal scale. The research utilized k-nearest neighbors clustering analysis to categorize respondents into groups aligning with the ILO’s decent work indicators. To assess changes in respondents’ income, paired Wilcoxon tests were employed.
Results: Exploratory data analysis indicates that Indicators 1 (Employment opportunities) and 7 (Equal opportunity and treatment in employment) align well with ILO’s indicators. Cluster analysis confirms that Indicators 1 and 7 share a high-performance group. In contrast, Indicators 6 (Stability and security of work) and 9 (Social security) exhibit the lowest alignment. There is no statistically significant income difference before and after becoming affiliate marketers.
Conclusion: Exploratory data analysis indicates that Indicators 1 (Employment opportunities) and 7 (Equal opportunity and treatment in employment) align well with ILO’s indicators. Cluster analysis confirms that Indicators 1 and 7 share a high-performance group. In contrast, Indicators 6 (Stability and security of work) and 9 (Social security) exhibit the lowest alignment. There is no statistically significant income difference before and after becoming affiliate marketers.
Keywords: Affiliate marketing, Decent work indicators, International Labor Organization, Social security
Navigating the Digital Marketplace: A Comprehensive Review of E-Commerce Trends, Challenges, and Innovations
Authors: Zangana Hewa , Natheer Yaseen Ali  , Ayaz khalid Mohammed
Published: April 2024

Background: Comprehensive exploration of e-commerce landscape; Insights drawn from diverse scholarly sources across disciplines.
Objective: Examine key themes, including COVID-19 impact, consumer behavior, business models, regulatory challenges, security, privacy, opportunities, and future trends in digital commerce.
Method: The methodology involved a comprehensive literature review spanning various disciplines to explore e-commerce. Scholarly sources were gathered from academic databases and journals, focusing on key themes like the COVID-19 impact, consumer behavior, business models, regulatory challenges, security, privacy, opportunities, and future trends. Data analysis identified patterns and trends, with findings organized into distinct sections. Synthesizing and interpreting the results within the e-commerce context, along with peer feedback, ensured the study's rigor and credibility.
Results: Uncover sustained shift in consumer preferences influenced by the pandemic; Provide insights into strategic approaches adopted by businesses in the digital marketplace.
Conclusion: Anticipate future e-commerce trajectory, discussing emerging trends like metaverse integration, AI, augmented reality shopping, voice commerce, and online-offline convergence; Serve as a guide for businesses navigating challenges, seizing opportunities, and aligning with emerging trends; Offer a comprehensive understanding of the dynamic nature of e-commerce in the digital age.
Keywords: Artificial Intelligence; Business; E-Commerce; Marketing.
MetaBIDx: a new computational approach to bacteria identification in microbiomes
Authors: Pham Diem-Trang , Phan Vinhthuy
Published: April 2024

Objectives: This study introduces MetaBIDx, a computational method designed to enhance species prediction in metagenomic environments. The method addresses the challenge of accurate species identification in complex microbiomes, which is due to the large number of generated reads and the ever-expanding number of bacterial genomes. Bacterial identification is essential for disease diagnosis and tracing outbreaks associated with microbial infections.

Methods: MetaBIDx utilizes a modified Bloom filter for efficient indexing of reference genomes and incorporates a novel strategy for reducing false positives by clustering species based on their genomic coverages by identified reads. The approach was evaluated and compared with several well-established tools across various datasets. Precision, recall, and F1-score were used to quantify the accuracy of species prediction.

Results: MetaBIDx demonstrated superior performance compared to other tools, especially in terms of precision and F1-score. The application of clustering based on approximate coverages significantly improved precision in species identification, effectively minimizing false positives. We further demonstrated that other methods can also benefit from our approach to removing false positives by clustering species based on approximate coverages.

Conclusion: With a novel approach to reducing false positives and the effective use of a modified Bloom filter to index species, MetaBIDx represents an advancement in metagenomic analysis. The findings suggest that the proposed approach could also benefit other metagenomic tools, indicating its potential for broader application in the field. The study lays the groundwork for future improvements in computational efficiency and the expansion of microbial databases.
Major adverse foot events and functional mobility deficits associated with diabetic neuropathy and nephropathy
Authors: Sinacore David R. , Jones Michael A. , Kline Paul W.
Published: April 2024

Aim: The purpose is to determine the risk ratios (RR) for both major adverse foot events (MAFEs) and the presence of moderate and severe functional mobility deficits in participants with diabetic peripheral neuropathy across the stages of chronic kidney disease (CKD).

Methods: We studied 284 participants with diabetes mellitus, peripheral neuropathy, and CKD. MAFEs including foot fracture, ulcerations, Charcot neuropathic arthropathy (CN), osteomyelitis, and minor foot amputations were collected from foot x-ray reports in the medical records of 152 participants; functional mobility deficits were assessed in 132 participants using the modified physical performance test (mPPT). Moderate mobility deficit was categorized as mPPT scores 22-29 and severe mobility deficit as < 22. Unadjusted and adjusted (age, body weight, race, HbA1c) RR were calculated across each stage of CKD, with stage 1 CKD used as the reference group.

Results: The RR for neuropathic foot fracture, CN, and diabetic foot ulceration remained consistent across CKD stages. The RR of minor amputation is greater in CKD stages 4 and 5. The RR of moderate or severe mobility deficit is greater in CKD stages 3 and 5 and in CKD stages 3, 4, and 5, respectively. An inverse association was observed between MAFE prevalence and mPPT scores across CKD stages.

Conclusion: Major adverse foot events and functional mobility deficits are prevalent in individuals with DPN and diabetic kidney disease. The risks for minor foot amputation and functional mobility deficits increase as early as stage 3 CKD and increase further in stages 4 and 5.
Pengaruh Pemberian Berbagai Macam Bahan Organik terhadap Ketersediaan Hara Nitrogen, Fosfor dan Kalium serta Serapan Nitrogen oleh Jagung (Zea mays L.) pada Tanah Ultisols
Authors: Mustaqim Ahmad , Ifansyah Hairil , Saidy Akhmad Rizalli
Published: April 2024

Ultisols distributes spreadly in Indonesia, but this soil has low fertility. Therefore, the application of organic matter (OM) is a common management to increase this soil fertility. The purpose of this study was to determine the role of different types of OM (originated from animals and plants) on the availability of nitrogen, phosphorus, and potassium as well as nitrogen uptake in maize in Ultisols. Soil with a mass of 5 kg without organic matter (control), with the application of chicken manure, cow manure, swallow manure, kirinyuh, babadotan, and kalopo manure were incubated in experimental pots for two weeks at 60% field capacity. After the completion of the incubation period, soil sub-sampling was carried out for the determination of available N, available P and exchangeable K. Furthermore, in each experimental pot, corn seeds were planted, and the observations of dried-weight of  corn and nitrogen uptake in corn were observed in a vegetative phase. The results showed that the application of different types of organic matter increased soil pH, nitrate content, exchangeable K, plant dried-weight and plant nitrogen uptake. The content of ammonium and available P was not affected by OM addition. The results also showed that OM derived from animals resulted in  higher increases in pH and exchangeable K than those derived from plants. However, OM derived from plants resulted in higher increases in nitrate, plant dry weight and better plant N uptake than OM from animal waste. The results of this study show that the application of OM results in increases  in some soil chemical properties, in which the degree of increases in soil chemical characteristics is controlled by the OM origin.
Pengaruh Pemberian Pupuk Guano dan Dolomit terhadap Ketersediaan Unsur Hara Posfor pada Tanah Podsolik
Authors: Puspitasari Indah Apriliana , Septiana Meldia , Razie Fakhrur
Published: April 2024

Podzolic soils have various problems including acidity of the soil (pH) and low soil nutrient content. This study aims to determine the effect of guano and dolomite on the availability of P nutrients in podsolik soils, and to determine the best treatment. This study was a pot experiment using a completely randomized design (CRD) with one factor, namely (P1 = Dolomite 2 t ha-1); (P2 = Guano Fertilizer 0,5 t ha-1); (P3 Guano Fertilizer 0.5 t ha-1 and Dolomite 2 t ha-1); (P4 = Guano Fertilizer 1 t ha-1 and Dolomite 2 t ha-1); (P5 Guano Fertilizer 1.5 t ha-1 and Dolomite 2 t ha-1). Each treatment was repeated 4 times, so there were 24 experimental units. The results showed that the application of dolomite and guano fertilizers had an effect on the available-P, pH, Exchangable Ca and Exchangable Al. However, it has no effect on Mg-dd in Podzolic soil. The application of dolomite and guano fertilizer with a dosage of 0.5 t ha-1 guano fertilizer and 2 t ha-1 dolomite was the best result for the availability of P, Exchangable Ca and pH.
THE RELATIONSHIP BETWEEN THE LEVEL OF KNOWLEDGE, ATTITUDES, AND ACTIONS WITH THE BEHAVIOR OF CHECKING THE DENTAL AND ORAL HEALTH OF PREGNANT WOMEN
Authors: Istiqamah Andi , Hatta Isnur , Wardani Ika Kusuma
Published: April 2024

Background: The number of dental and oral health the Indonesian is 57.6%, which is dominated by women (58.5%), especially women of productive age. During pregnancy, there are physical changes, hormonal changes, and behavioral changes, resulting in the changes for the maintenance of dental and oral hygiene. It becomes worse due to an increase in the estrogen and progesteronehormones. Purpose: To analyze the relationship between the level of knowledge, attitudes, and actions with the behavior of checking the dental and oral health of pregnant women. Methods: This study used an analytical observation with a cross-sectional research design. The samples of the study were calculated using the sample size correlation formula. The population in this study were all pregnant women from December 2020 to May 2021,at the Tana Lili Health Center, North Luwu Regency and the samples used were 34 respondents. Results: 70% of pregnant women have a sufficient level of knowledge, 79% of pregnant women have a quite good attitude, 68% of pregnant women have bad actions, and 85% of pregnant women have less behavior. The Spearman correlation test showed a significant relationship between the level of knowledge and the behavior of checking the oral health of pregnant women (p = 0.003), and the strength of the correlation was moderate (r = 0.497). There was a significant relationship between attitudes and behavior of checking the dental and oral health of pregnant women (p=0.023), and the strength of the correlation was low (r=0.390). The sig value for the relationship between action and behavior was (p = 0.009) meaning that there was a significant relationship between the action and the behavior of checking the dental and oral health of pregnant women with a value (r = 0.439) meaning that the correlation strength was moderate. Conclusion: There is a significant relationship between the level of knowledge, attitudes, and actions and the behavior of checking the dental and oral health of pregnant women. Keywords: Action, Attitude, Behavior of checking the dental and oral health, Knowledge
RELATIONSHIP OF SANITATION AND SOCIO ECONOMIC STATUS OF PARENTS TO CARIES SEVERITY
Authors: Ilvani Thine Putri Bestari , Hatta Isnur , Adhani Rosihan
Published: April 2024

Background: Riskesdas stated the prevalence of caries in children aged 5-9 years in Indonesia in 2018 was 92.6%. External caries factors consist of environment, behavior, health services, and heredity. The physical environment is one of the health environments that contributes the most to health status, including dental and oral health. Sanitation with the Community-Based Total Sanitation (STBM) program is part of the physical environment. The STBM pillars consist of: stopping open defecation, washing hands with soap, drinking water and food management, waste management, and liquid waste management. Behavioral predisposing factors include parents' socioeconomic status which is determined by education, occupation, and income. Socioeconomic status affects the ability of parents to care for their children's teeth. Purpose: To analyze the relationship between sanitation and socioeconomic status of parents on the severity of caries in kindergarten children. Methods: This research is an analytic observational study with a cross-sectional method. The respondents of this study were 49 children of TK Al Hidayah in Mandiangin Barat Village, Banjar Regency, South Kalimantan Province. Data collection includes questionnaires and the def-t assessment sheet. Results: Spearman correlation test p=0.435 showed no relationship between sanitation and caries severity. Spearman correlation test p=0.0001 showed that there was a relationship between the socioeconomic status of parents and the severity of caries. The correlation coefficient -0.519 indicates a medium strength relationship in the opposite direction. Conclusion: There is no relationship between sanitation and caries severity. There is a relationship between the socioeconomic status of parents with the severity of caries.  Keywords: def-t index, Socio-economic, STBM.
Dramaturgi Identitas Diri Biduan Waria
Authors: Solehah Nor , An’amta Dimas Asto Aji
Published: April 2024

A transvestite who performs dramaturgical performances when interacting with others and manages the impression he hopes to grow on others of him, through a self-performance that experiences the setting in public. This study aims to find out how transvestite biduan dramaturgizes his identity in the perspective of dramaturgy and the identity of Erving Goffman. In this study, researchers used a type of qualitative research with a phenomenological approach. This research was conducted in Kumai District, West Kotawaringin Regency. The study time starts in January-April 2023. Data collection techniques use observation, interviews and documentation. The main informants in this study were 4 waria who worked as midwives. Data analysis techniques are data reduction, data display and data verification. The results showed that transvestites used masks to cover their faces. Or in other words, some transvestites play different roles between their backstage and frontstage due to their identity as transvestites. The front stage of waria singers almost all plays the front stage well, which is done like dressing men in front of the family and praying in the men's row. The stage behind the transvestite singer truly expresses herself as a woman with all the personality and changes in appearance, including her distinctive style of dress and way of speaking. So then waria expressed this sexual orientation selection through the way they dressed, where waria subjects in this study stated that their sexual orientation was directed towards men.
Paradoks Hubungan Petani Padi dan Tengkulak di Desa Sepanjang Kecamatan Glenmore Kabupaten Banyuwangi
Authors: Aflah Bryan Marshal , Suhartini Elly , Arifiyanti Jati
Published: April 2024

Rice farming in the Sepanjang village's main income, but not all farmers have direct access to marketers such as factories, especially peasant who have limited land who ultimately choose to sell crops to middlemen using a patron-client system. The aim of this research is to find out, describe and analyze the paradoxical relationship between peasant and middlemen. The research was carried out in villages along Glenmore District, Banyuwangi Regency. The research method used is qualitative with a phenomenological approach. The research was carried out in April - June 2023. In the process of selling to middlemen, peasant get positive benefits such as getting loans, transportation accommodation and guaranteed marketing networks. However, in their relationship with middlemen, peasant also experience several negative things, such as fraud committed by middlemen, such as rounding off weigh figures, rounding off purchase prices and manipulation during the gabug or tare testing process. In this position, the peasant position becomes a paradox, namely that it lies in the condition where the peasant gets positive and negative sides in the relationship with middlemen simultaneously. In the theoretical realm, this research can be a reference point in developing research on the existence and relationships of patron-clients. In the practical realm, this research can be a point of consideration or considerations for determining regional and government policies, especially in the agricultural sector.
Shèng Nǚ: Stereotip Perempuan Lajang di China Pada Film “The Last Woman Standing”
Authors: Pratiwi R Hervita Thalia , Erwani Intan
Published: April 2024

China is a country with a strong patriarchal culture and weakens the position of women, so that when women rebel and dare to fight against standards in society, they will be faced with stereotypes and discrimination. This research aims to know the forms of stereotypes experienced by Chinese women in the current era due to the mismatch between their attitudes or life choices and the norms that apply in society. This research examines the stereotypes experienced by single women in China to get the label Shèng Nǚ. The data source for this research is the film “The Last Woman Standing”, as well as other research supporting data sources. The research was conducted using qualitative descriptive methods. The theory used to analyze the research results is existential feminism from Beauvoir (1956). This research found stereotypical forms of single women in China shown in the film "The Last Woman Standing", namely: 1) rejecting marriage; 2) does not need men; and, 3) voters. In fact, women do not reject marriage, it's just that women wait for the right time and the right person for their future. Furthermore, single women are afraid that men are not equal to them, which results in differences in views on life principles and inequality that worsens their situation in the future. Women in this era have the right to make their life choices and not depend on other people, especially men. The results of this research are a reference for the struggle for the existence of single women who are stereotyped and discriminated against by patriarchal communities that think women should be below men.
Kampung Janda: Konstruksi Sosial Media Online Terhadap Perempuan yang Pernah Bersuami di Kota Banjarbaru
Authors: Aprilasari Salma , Hakim Arif Rahman
Published: April 2024

The existence of public perception about the news of the nickname “widow village” in Banjarbaru City, apparently caused pros and cons in the community. This research aims to find out the social construction in online media towards “widow village”. The type of research is descriptive qualitative using a constructivism paradigm approach. Data collection used observation, interviews and documentation. Data analysis used Robert N. Entman's framing analysis model. The theories used are social construction of mass media and critical discourse analysis. The results of this study show that in the news search column delivered by online media such as Tribunnews.com, there is a regular distribution of news, varied titles and erratic release dates. After further investigation, there are only two news contents that are slightly different in the Tribunnews.com media. Meanwhile, the results of fieldwork in the dialectical process of social construction are shown in three simultaneous processes, namely internalization, objectivation, and externalization. Internalization shows how the outside community reflects the “widow village” from interactions and actions. Objectivation shows how people begin to understand what is constructed. Externalization shows how people show their views or attitudes when they have been constructed on the news of “widow village” at some time later. The conclusion that can be drawn is that some of the elements highlighted from the media's perspective are considered not only gender bias but also morality issues, such as one of the widowed female informants who is still angry and difficult to return to talking about the topic. It is important, as much as possible, to sort out which news is good and true with false or lies, don't just focus on images and news content and easily demean fellow beings, whether women or men.
Community Perception Related to Flood Mitigation in Kalodran Village, Walantaka District, Serang City, Banten
Authors: Handayani Nur Purwati , Alwin Alwin
Published: April 2024

Tujuan dari penelitian ini adalah untuk mengetahui bagaimana persepsi masyarakat terkait mitigasi (pra bencana) banjir di Kelurahan Kalodran Kecamatan Walantaka Kota Serang Provinsi Banten. Penelitian menggunakan metode deskriptif dengan pendekatan kuantitatif. Populasi dalam penelitian ini adalah 74 masyarakat berdasarkan jumlah KK di wilayah yang terkena dampak banjir di Kelurahan Kalodran Kecamatan Walantaka. Teknik pengumpulan data pada penelitian ini menggunakan observasi lapangan, penyebaran kuesioner (angket), dan dokumentasi. Analisis data yang digunakan pada penelitian ini adalah deskriptif kuantitatif berdasarkan hasil presentase. Hasil penelitian menunjukan bahwa Persepsi masyarakat terkait mitigasi (pra bencana) banjir di Kelurahan Kalodran Kecamatan Walantaka, Kota Serang, Banten termasuk dalam kategori sedang dengan presentase 62,4% menjawab kurang setuju dan belum mengetahui Langkah-langkah yang harus dilakukan sebelum terjadinya bencana banjir, dikarenakan tidak adanya pemahaman dan pengetahuan masyarakat terkait mitigasi pra bencana banjir serta tidak adanya kegiatan sosialiasi atau pelatihan yang diselenggarakan oleh Lembaga yang berwenang, namun dari dukungan masyarakat yang memilih setuju artinya jika dilakukannya kegiatan sosialisasi terkait mitigasi bencana di Kelurahan Kalodran maka masyarakat akan mendukung kegiatan tersebut.