bluuebunny/crossref_metadata_2025 huggingface dataset:
Does cutting down on your food consumption lead to a net improvement in nutritional intake? A panel data approach using data from the UK Biobank
Authors: Wilson Luke B. , Pryce Robert , Moore Esther C. , Burke Lucy , Breeze Penny
Published: November 2023

Background

Food diets are complex and a policy targeting one item of a person’s diet does not affect their nutritional intake in a solely additive or subtractive manner. Policies tackling unhealthy diets are more likely to be adopted by governments if there is robust evidence to support them. To evaluate dietary policies, it is important to understand the correlations and interdependencies between food groups, as these can lead to unintended negative consequences. We aimed to see whether reductions in consumption of a particular group is related to a net improvement in nutritional intake, after taking into account patterns of consumption and substitution across food groups.

Methods

Detailed dietary data was collected using a 24-h online dietary assessment from the UK Biobank and Oxford Web Q (n = 185,611). We used panel data fixed effects methods to estimate changes in energy, saturated fat, total sugar, and fibre following a 100gram reduction across 44 food groups. We compare these estimates against the average nutritional value of that food group from the UK National Diet and Nutrition Survey.

Results

We find evidence of variation in whether a food is compensated between the main confectionery products. Crisps, savoury snacks, and sugar confectionery are less likely to be compensated, whereas chocolate confectionery, biscuits, and buns/cakes/pastries and pies are compensated. The result is particularly striking for chocolate confectionery which shows that while chocolate confectionery often has a high energy content, eating less chocolate confectionery is not associated with an equal reduction in energy. Instead, we find individuals switch or compensate for their reduction in chocolate confectionery consumption with other high energy food items.

Conclusions

We find that sugar confectionery and crisps and savoury snacks are less likely to result in substitution than chocolate confectionery. This would suggest that food policies aiming to reduce the consumption of these food groups are more likely to result in overall lower consumption of unhealthy foods.
Addressing mental health problems among persons without stable housing in the context of the COVID-19 pandemic: study protocol for a randomised trial. RESPOND – France
Authors: Melchior Maria , Figueiredo Natasha , Roversi Aurélia , Dubanchet Alexandre , Bui Eric , Vadell-Martínez Julian , Barbui Corrado , Purgato Marianna , Ayuso-Mateos José Luis , Mediavilla Roberto , McDaid David , Park A-La , Petri-Romão Papoula , Kalisch Raffael , Nicaise Pablo , Lorant Vincent , Sijbrandij Marit , Witteveen Anke B. , Bryant Richard , Felez Mireia , Underhill James , Pollice Giulia , Tortelli Andrea
Published: November 2023

Background

The COVID-19 pandemic has had an impact on population-wide mental health and well-being. Although people experiencing socioeconomic disadvantage may be especially vulnerable, they experience barriers in accessing mental health care. To overcome these barriers, the World Health Organization (WHO) designed two scalable psychosocial interventions, namely the web-based Doing What Matters in Times of Stress (DWM) and the face-to-face Problem Management Plus (PM+), to help people manage stressful situations. Our study aims to test the effectiveness of a stepped-care program using DWM and PM + among individuals experiencing unstable housing in France – a majority of whom are migrant or have sought asylum.

Methods

This is a randomised controlled trial to evaluate the effectiveness and cost effectiveness of a stepped-care program using DWM and PM + among persons with psychological distress and experiencing unstable housing, in comparison to enhanced care as usual (eCAU). Participants (N = 210) will be randomised to two parallel groups: eCAU or eCAU plus the stepped-care program. The main study outcomes are symptoms of depression and anxiety measured using the Patient Health Questionnaire Anxiety and Depression Scale (PHQ-ADS).

Discussion

This randomised controlled trial will contribute to a better understanding of effective community-based scalable strategies that can help address the mental health needs of persons experiencing socioeconomic disadvantage, whose needs are high yet who frequently have limited access to mental health care services.

Trial registration

this randomised trial has been registered at ClinicalTrials.gov under the number NCT05033210.
Erythropoietin suppresses osteoblast apoptosis and ameliorates steroid-induced necrosis of the femoral head in rats by inhibition of STAT1-caspase 3 signaling pathway
Authors: Cai Tingwen , Chen Siyuan , Wu Chenghu , Lou Chao , Wang Weidan , Lin Chihao , Jiang Hongyi , Xu Xinxian
Published: November 2023

Background

Steroid-induced avascular necrosis of the femoral head (SANFH) is characterized by osteoblast apoptosis, leading to a loss of bone structure and impaired hip joint function. It has been demonstrated that erythropoietin (EPO) performs a number of biological roles.

Objective

We examined the effects of EPO on SANFH and its regulation of the STAT1-caspase 3 signaling pathway.

Method

In vitro, osteoblasts were treated with dexamethasone (Dex) or EPO. We identified the cytotoxicity of EPO by CCK-8, the protein expression of P-STAT1, cleaved-caspase9, cleaved-caspase3, Bcl-2, BAX, and cytochrome c by Western blotting, and evaluated the apoptosis of osteoblasts by flow cytometry. In vivo, we analyzed the protective effect of EPO against SANFH by hematoxylin and eosin (H&E), Immunohistochemical staining, and Micro-computed tomography (CT).

Results

In vitro, EPO had no apparent toxic effect on osteoblasts. In Dex-stimulated cells, EPO therapy lowered the protein expression of BAX, cytochrome c, p-STAT1, cleaved-caspase9, and cleaved-caspase3 while increasing the expression of Bcl-2. EPO can alleviate the apoptosis induced by Dex. In vivo, EPO can lower the percentage of empty bone lacunae in SANFH rats.

Conclusion

The present study shows that EPO conferred beneficial effects in rats with SANFH by inhibiting STAT1-caspase 3 signaling, suggesting that EPO may be developed as a treatment for SANFH.
Augmentation of single tooth extraction socket with deficient buccal walls using bovine xenograft with platelet-rich fibrin membrane
Authors: Elbanna Riham Mohamed , Abdelaziz Medhat Sameh , Alameldeen Hesham Ebrahim
Published: November 2023

Background

Different techniques and materials such as bone grafts and bioactive agents have been used for alveolar ridge augmentation in extraction sockets with a defective wall, there is not a specific material or technique that has resulted in superior outcomes or prevented total bone loss.

Objectives

This clinical study aims to evaluate radiographically the effectiveness of using bovine xenograft with platelet-rich fibrin (PRF) membrane on vertical and horizontal alveolar ridge dimensional changes following tooth extraction that are complicated by buccal bone loss.

Materials and methods

This study was conducted in Egypt on fourteen patients with a single posterior tooth indicated for extraction. A preoperative cone-beam computed tomography (CBCT) scan confirmed more than 50% loss in buccal bone in each tooth. Extraction sockets were packed with minced PRF clots mixed with a bovine xenograft. Each extraction socket was sealed by PRF membranes. CBCT scans, performed before tooth extraction and after 6 months, were used to assess alveolar ridge changes both vertically and horizontally.

Results

There was a significant gain in the buccal and middle of the extraction socket bone height, recording 86.01% (6.33 mm) and 206.45% (9.6 mm), respectively. There was an insignificant bone loss in the lingual bone height and width, recording − 8.49% (-1.06 mm) and − 13.39% (1.05 mm), respectively. The results also showed a non-significant decrease in alveolar bone density (-14.06%) between pre-operative bone present apical to the extraction socket and newly formed bone inside the socket.

Conclusions

Ridge preservation/augmentation techniques using a bone graft mixed with PRF and covered by PRF membranes in fresh extraction sockets complicated by the loss of buccal bone result in buccal bone augmentation and a reduction in horizontal and vertical ridge collapse after tooth extraction.

Clinical relevance

The bovine xenograft in conjunction with PRF can be used immediately after extraction for ridge preservation, providing adequate bone width and height for implant placement.
Digital measurement method for comparing the absolute marginal discrepancy of three-unit ceramic fixed dental prostheses fabricated using conventional and digital technologies
Authors: Liang Shanshan , Yuan Fusong , Li Deli , Jia Lu , Sun Yuchun
Published: November 2023

Background

In clinical practice, control of the marginal fit of fixed dental prostheses is hindered by evaluation method, which needs to be further improved to increase its clinical applicability. This study aimed to quantitatively analyze the absolute marginal discrepancy of three-unit ceramic fixed dental prostheses fabricated by conventional and digital technologies using a digital measurement method based on the digital impression technology and open source software.

Methods

A digital workflow and the conventional impression combined with the lost-wax heat-pressed technique were adopted to separately fabricate 10 glass ceramic fixed dental prostheses. Three-dimensional data for the abutments, fixed dental prostheses, and fixed dental prostheses seated on the abutments, were obtained using a dental scanner. The two datasets were aligned using registration technology, specifically “multi-points registration” and “best fit alignment,” by reverse engineering software. Subsequently, the three-dimensional seated fit between the fixed dental prostheses and abutments were reconstructed. The margin of the abutment and crown was extracted using edge-sharpening and other functional modules, and the absolute marginal discrepancy was measured by the distance between the margin of the abutment and crown. One-way analysis of variance was used to statistically analyze the measurement results.

Results

Using the digital measurement method, the mean value of absolute marginal discrepancy for fixed dental prostheses fabricated by the conventional method was 106.69 ± 6.46 μm, and that fabricated by the digital workflow was 102.55 ± 6.96 μm. The difference in the absolute marginal discrepancy of three-unit all-ceramic fixed dental prostheses fabricated using the two methods was not statistically significant (p > 0.05).

Conclusions

The digital measurement method for absolute marginal discrepancy was preliminarily established based on open source software and applied in three-unit ceramic fixed dental prostheses. The absolute marginal discrepancy of three-unit ceramic fixed dental prostheses fabricated using digital technology was comparable to that of conventional technique.
The influence of core-build up materials on biaxial flexural strength of monolithic strength-gradient zirconia; an in-vitro study
Authors: Musa Dina B. , Ereifej Nadia S.
Published: November 2023

Background

Since their introduction, there has been limited research regarding the mechanical properties of novel strength-gradient monolithic zirconia. In addition to that, studies evaluating the effect of different core-build materials on the strength of indirect restorations are scarce. Therefore, the aim of this study was to investigate the effect of using different core build-up materials on biaxial flexural strength of a new monolithic multilayered zirconia material.

Methods

Forty zirconia discs were fabricated from IPS e.max ZirCAD Prime (Ivoclar Vivadent AG, Schaan, Liechtenstein) and divided into 2 groups (n = 20). Forty composite discs were prepared from Tetric N-Ceram (Ivoclar Vivadent AG, Schaan, Liechtenstein) and MultiCore Flow (Ivoclar Vivadent AG, Schaan, Liechtenstein). The zirconia discs were adhesively cemented to the 2 types of composite forming 2 groups (Zirconia-Tetric N-Ceram and Zirconia-MultiCore Flow). Biaxial flexural strength was determined using a piston-on-3-ball test. The data were statistically analyzed with an independent t-test for significant differences (p = 0.05).

Results

Tetric N-Ceram had significantly higher strength than MultiCore Flow (p < 0.001) but no statistically significant differences were found in strength values between Zirconia-Tetric N-Ceram and Zirconia-MultiCore Flow bilayered samples (p = 0.27).

Conclusions

It was concluded that although the tested composite core materials significantly differ in their biaxial flexural strength values, they had no influence on the biaxial flexural strength of the overlying zirconia.
Research and practice of the BOPPPS teaching model based on the OBE concept in clinical basic laboratory experiment teaching
Authors: Li Pei , Lan Xiaopeng , Ren Lin , Xie Xiaoping , Xie Haitao , Liu Shuangquan
Published: November 2023

"Clinical basic inspection technology" is one of the essential courses in the medical laboratory profession. Combining the characteristics of the discipline itself, the research and practice of the BOPPPS model based on the OBE concept in clinical basic laboratory experiment teaching are discussed, and the reform of in teaching objectives, teaching contents, and teaching design path is implemented. The "student-centered" teaching process is divided into six stages: before, during, and after class, and the teaching process is continuously improved to achieve the desired teaching effect. Results of the experiment teaching show that the model has improved students' active participation and developed their clinical thinking skills, and more than 95% of students are satisfied with this teaching model.
Experiences of person-centered care for sundown syndrome among nurses and nurse aides in dementia special care units: a qualitative study
Authors: Huang Su-Fei , Wang Bow-Yin , Liao Jung-Yu
Published: November 2023

Background

To explore the response and management experiences of nurses and nurse aides in dementia special care units when caring for residents with sundown syndrome based on the person-centered care model.

Methods

Focus group interviews were conducted among nurses and nurse aides from four dementia special care units that have been accredited by the Ministry of Health and Welfare in Taiwan. Content analysis was used for data analysis.

Results

The 29 nurses and nurse aides were recruited to participate in the study. Analysis of interview content revealed six themes, identifying the intra-individual, inter-individual, and organizational dimensions. The central topic was commitment. Under the umbrella of commitment, six themes including self-preparation, non-suppression, diversion, pacification, continuity of meeting, and collaboration, which had 18 subthemes, emerged as responsive care practices for person-centered care when supporting residents with sundown syndrome.

Conclusions

The findings provide responsive care practices based on person-centered care for people living with dementia who develop sundown syndrome. The study can inform practices for quality of care for dementia in long-term care institutions and contribute to the development of materials for nursing training and education.
Balancing the interplay of histone deacetylases and non-coding genomes: a step closer to understand the landscape of cancer treatment
Authors: Pu Jingjing , Liu Ting , Sharma Amit , Schmidt-Wolf Ingo G. H.
Published: November 2023

Histone deacetylase (HDAC) inhibitors have enormous therapeutic potential as effective epigenetic regulators, and now with the focus on the selective HDAC6 inhibitor in ongoing clinical trials, more advantages over other non-selective pan-HDAC inhibitors are foreseeable. As it is of paramount importance to understand the complex regulatory web of mutual interactions involving epigenetic machinery and non-coding genome in regulating gene expression, herein, we investigated the intriguing interactions between HDAC6-induced lncRNA (LINC00152) and its possible sponge miRNA (hsa-miR-499a-5p) in multiple myeloma.
Fatty acids abrogate the growth-suppressive effects induced by inhibition of cholesterol flux in pancreatic cancer cells
Authors: Li Yuchuan , Amrutkar Manoj , Finstadsveen Anette Vefferstad , Dalen Knut Tomas , Verbeke Caroline S. , Gladhaug Ivar P.
Published: November 2023

Background

Despite therapeutic advances, the prognosis of pancreatic ductal adenocarcinoma (PDAC) remains extremely poor. Metabolic reprogramming is increasingly recognized as a key contributor to tumor progression and therapy resistance in PDAC. One of the main metabolic changes essential for tumor growth is altered cholesterol flux. Targeting cholesterol flux appears an attractive therapeutic approach, however, the complex regulation of cholesterol balance in PDAC cells remains poorly understood.

Methods

The lipid content in human pancreatic duct epithelial (HPDE) cells and human PDAC cell lines (BxPC-3, MIA PaCa-2, and PANC-1) was determined. Cells exposed to eight different inhibitors targeting different regulators of lipid flux, in the presence or absence of oleic acid (OA) stimulation were assessed for changes in viability, proliferation, migration, and invasion. Intracellular content and distribution of cholesterol was assessed. Lastly, proteome profiling of PANC-1 exposed to the sterol O-acyltransferase 1 (SOAT1) inhibitor avasimibe, in presence or absence of OA, was performed.

Results

PDAC cells contain more free cholesterol but less cholesteryl esters and lipid droplets than HPDE cells. Exposure to different lipid flux inhibitors increased cell death and suppressed proliferation, with different efficiency in the tested PDAC cell lines. Avasimibe had the strongest ability to suppress proliferation across the three PDAC cell lines. All inhibitors showing cell suppressive effect disturbed intracellular cholesterol flux and increased cholesterol aggregation. OA improved overall cholesterol balance, reduced free cholesterol aggregation, and reversed cell death induced by the inhibitors. Treatment with avasimibe changed the cellular proteome substantially, mainly for proteins related to biosynthesis and metabolism of lipids and fatty acids, apoptosis, and cell adhesion. Most of these changes were restored by OA.

Conclusions

The study reveals that disturbing the cholesterol flux by inhibiting the actions of its key regulators can yield growth suppressive effects on PDAC cells. The presence of fatty acids restores intracellular cholesterol balance and abrogates the alternations induced by cholesterol flux inhibitors. Taken together, targeting cholesterol flux might be an attractive strategy to develop new therapeutics against PDAC. However, the impact of fatty acids in the tumor microenvironment must be taken into consideration.
Sex difference of pre- and post-natal exposure to six developmental neurotoxicants on intellectual abilities: a systematic review and meta-analysis of human studies
Authors: Goodman Carly V. , Green Rivka , DaCosta Allya , Flora David , Lanphear Bruce , Till Christine
Published: November 2023

Background

Early life exposure to lead, mercury, polychlorinated biphenyls (PCBs), polybromide diphenyl ethers (PBDEs), organophosphate pesticides (OPPs), and phthalates have been associated with lowered IQ in children. In some studies, these neurotoxicants impact males and females differently. We aimed to examine the sex-specific effects of exposure to developmental neurotoxicants on intelligence (IQ) in a systematic review and meta-analysis.

Method

We screened abstracts published in PsychINFO and PubMed before December 31st, 2021, for empirical studies of six neurotoxicants (lead, mercury, PCBs, PBDEs, OPPs, and phthalates) that (1) used an individualized biomarker; (2) measured exposure during the prenatal period or before age six; and (3) provided effect estimates on general, nonverbal, and/or verbal IQ by sex. We assessed each study for risk of bias and evaluated the certainty of the evidence using Navigation Guide. We performed separate random effect meta-analyses by sex and timing of exposure with subgroup analyses by neurotoxicant.

Results

Fifty-one studies were included in the systematic review and 20 in the meta-analysis. Prenatal exposure to developmental neurotoxicants was associated with decreased general and nonverbal IQ in males, especially for lead. No significant effects were found for verbal IQ, or postnatal lead exposure and general IQ. Due to the limited number of studies, we were unable to analyze postnatal effects of any of the other neurotoxicants.

Conclusion

During fetal development, males may be more vulnerable than females to general and nonverbal intellectual deficits from neurotoxic exposures, especially from lead. More research is needed to examine the nuanced sex-specific effects found for postnatal exposure to toxic chemicals.
Comprehensive insights into the mechanism of keratin degradation and exploitation of keratinase to enhance the bioaccessibility of soybean protein
Authors: Zhou Beiya , Guo Yandong , Xue Yaju , Ji Xiuling , Huang Yuhong
Published: November 2023

Keratin is a recalcitrant protein and can be decomposed in nature. However, the mechanism of keratin degradation is still not well understood. In this study, Bacillus sp. 8A6 can completely degrade the feather in 20 h, which is an efficient keratin degrader reported so far. Comprehensive transcriptome analysis continuously tracks the metabolism of Bacillus sp. 8A6 throughout its growth in feather medium. It reveals for the first time how the strain can acquire nutrients and energy in an oligotrophic feather medium for proliferation in the early stage. Then, the degradation of the outer lipid layer of feather can expose the internal keratin structure for disulfide bonds reduction by sulfite from the newly identified sulfite metabolic pathway, disulfide reductases and iron uptake. The resulting weakened keratin has been further proposedly de-assembled by the S9 protease and hydrolyzed by synergistic effects of the endo, exo and oligo-proteases from S1, S8, M3, M14, M20, M24, M42, M84 and T3 families. Finally, bioaccessible peptides and amino acids are generated and transported for strain growth. The keratinase has been applied for soybean hydrolysis, which generates 2234 peptides and 559.93 mg/L17 amino acids. Therefore, the keratinases, inducing from the poultry waste, have great potential to be further applied for producing bioaccessible peptides and amino acids for feed industry.
Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production
Authors: Gao Le , Meng Jiao , Dai Wuling , Zhang Zhaokun , Dong Haofan , Yuan Qianqian , Zhang Wuyuan , Liu Shuguang , Wu Xin
Published: November 2023

Single-cell protein (SCP) production in the methylotrophic yeast Pichia pastoris has the potential to achieve a sustainable protein supply. However, improving the methanol fermentation efficiency and reducing carbon loss has been a long-standing challenge with far-reaching scientific and practical implications. Here, comparative transcriptomics revealed that PAS_0305, a gene directly associated with cell wall thickness under methanol stress, can be used as a target for unlocking cell wall sensors. Intracellular trehalose accumulation confirmed that cell wall sensors were activated after knocking out PAS_0305, which resulted in increased cell wall permeability. Genome-wide signal perturbations were transduced through the HOG module and the CWI pathway, which was confirmed to connected by Pbs2-Mkk. As a consequence of CWI pathway activation, ΔPAS_0305 elicited a rescue response of cell wall remodeling by increasing the β-1,3-glucan content and decreasing the chitin/mannose content. Remarkably, perturbations in global stress signals led to a fine-tuning of the metabolic network of ΔPAS_0305, resulting in a superior phenotype with highest crude protein and methanol conversion rate of 67.21% and 0.46 gDCW/g. Further genome-scale metabolic models were constructed to validate the experimental results, confirming that unlocking cell wall sensors resulted in maximized flux from methanol towards SCP and effectively addressing the issue of carbon loss in methanol fermentation. This work sheds new light on the potential of manipulating cellular signaling pathways to optimize metabolic networks and achieve exceptional phenotypic characteristics, providing new strategies for constructing versatile cell factories in P. pastoris.
1H-NMR based-metabolomics reveals alterations in the metabolite profiles of chickens infected with ascarids and concurrent histomonosis infection
Authors: Oladosu Oyekunle John , Correia Banny Silva Barbosa , Grafl Beatrice , Liebhart Dieter , Metges Cornelia C. , Bertram Hanne Christine , Daş Gürbüz
Published: November 2023

Background

Gut infections of chickens caused by Ascaridia galli and Heterakis gallinarum are associated with impaired host performance, particularly in high-performing genotypes. Heterakis gallinarum is also a vector of Histomonas meleagridis that is often co-involved with ascarid infections. Here, we provide a first insight into the alteration of the chicken plasma and liver metabolome as a result of gastrointestinal nematode infections with concomitant histomonosis. 1H nuclear magnetic resonance (1H-NMR) based-metabolomics coupled with a bioinformatics analysis was applied to explore the variation in the metabolite profiles of the liver (N = 105) and plasma samples from chickens (N = 108) experimentally infected with A. galli and H. gallinarum (+H. meleagridis). This was compared with uninfected chickens at different weeks post-infection (wpi 2, 4, 6, 10, 14, 18) representing different developmental stages of the worms.

Results

A total of 31 and 54 metabolites were quantified in plasma and aqueous liver extracts, respectively. Statistical analysis showed no significant differences (P > 0.05) in any of the 54 identified liver metabolites between infected and uninfected hens. In contrast, 20 plasma metabolites including, amino acids, sugars, and organic acids showed significantly elevated concentrations in the infected hens (P < 0.05). Alterations of plasma metabolites occurred particularly in wpi 2, 6 and 10, covering the pre-patent period of worm infections. Plasma metabolites with the highest variation at these time points included glutamate, succinate, trimethylamine-N-oxide, myo-inositol, and acetate. Differential pathway analysis suggested that infection induced changes in (1) phenylalanine, tyrosine, and tryptophan metabolism, (2) alanine, aspartate and glutamate metabolism; and 3) arginine and proline metabolism (Pathway impact > 0.1 with FDR adjusted P-value < 0.05).

Conclusion

In conclusion, 1H-NMR based-metabolomics revealed significant alterations in the plasma metabolome of high performing chickens infected with gut pathogens—A. galli and H. gallinarum. The alterations suggested upregulation of key metabolic pathways mainly during the patency of infections. This approach extends our understanding of host interactions with gastrointestinal nematodes at the metabolic level.
Helicobacter pylori infection attenuates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in C57/BL6 mice
Authors: Wang Shuxian , Wang Xiaokang , Liu Jiaqi , Li Yaqian , Sun Minghui , Zhu Guoqiang , Zhu Xiaofang
Published: November 2023

Background

Although numerous studies have suggested a negative correlation between Helicobacter pylori (H. pylori) infection and allergies, there has been limited research on the relationship between H. pylori infections and atopic dermatitis (AD). The present study aimed to investigate the effects of H. pylori infection in an AD mouse model and identify potential mechanisms related to type 2 immunity, skin barrier defects, and pruritus.

Methods

A model of AD-like symptoms was established with 2,4-dinitrochlorobenzene (DNCB) after infection of the gastric cavity with H. pylori. Analysis of the expression of key inflammatory cytokines and serum levels of immunoglobulin E (IgE) was based on enzyme-linked immunosorbent assay (ELISA). The expression of filaggrin (FLG) and loricrin (LOR) were analyzed by immunohistochemistry staining. The evaluation of STAT1, STAT3, phosphorylated STAT1 (phospho-STAT1), and phosphorylated STAT3 (phospho-STAT1) expression levels in skin lesions was performed using western blot.

Results

The present study showed that the H. pylori-positive AD group (HP+AD+) exhibited milder skin lesions, including erythema, erosion, swelling, and scaling, than the H. pylori-negative AD group (HP−AD+). Additionally, HP+AD+ displayed lower levels of IgE in serum, and downregulated expression of interleukins 4 and 31 (IL-4 and IL-31) in serum. Furthermore, HP+AD+ demonstrated higher expression of filaggrin and loricrin than HP−AD+. Notably, H. pylori significantly reduced the amount of phosphorylated STAT1 and STAT3.

Conclusion

Helicobacter pylori infection negatively regulates the inflammatory response by affecting inflammatory factors in the immune response, and repairs the defective epidermal barrier function. In addition, H. pylori infection may reduce IL-31, thereby alleviating pruritus. These effects may be associated with the inhibition of JAK–STAT signaling activation.
LppA is a novel plasminogen receptor of Mycoplasma bovis that contributes to adhesion by binding the host extracellular matrix and Annexin A2
Authors: Liu Shuang , Li Zhangcheng , Lan Shimei , Hao Huafang , Jin Xiangrui , Liang Jinjia , Baz Ahmed Adel , Yan Xinmin , Gao Pengcheng , Chen Shengli , Chu Yuefeng
Published: November 2023

Mycoplasma bovis is responsible for various inflammatory diseases in cattle. The prevention and control of M. bovis are complicated by the absence of effective vaccines and the emergence of multidrug-resistant strains, resulting in substantial economic losses worldwide in the cattle industry. Lipoproteins, vital components of the Mycoplasmas cell membrane, are deemed potent antigens for eliciting immune responses in the host upon infection. However, the functions of lipoproteins in M. bovis remain underexplored due to their low sequence similarity with those of other bacteria and the scarcity of genetic manipulation tools for M. bovis. In this study, the lipoprotein LppA was identified in all examined M. bovis strains. Utilizing immunoelectron microscopy and Western blotting, it was observed that LppA localizes to the surface membrane. Recombinant LppA demonstrated dose-dependent adherence to the membrane of embryonic bovine lung (EBL) cells, and this adhesion was inhibited by anti-LppA serum. In vitro binding assays confirmed LppA’s ability to associate with fibronectin, collagen IV, laminin, vitronectin, plasminogen, and tPA, thereby facilitating the conversion of plasminogen to plasmin. Moreover, LppA was found to bind and enhance the accumulation of Annexin A2 (ANXA2) on the cell membrane. Disrupting LppA in M. bovis significantly diminished the bacterium’s capacity to adhere to EBL cells, underscoring LppA’s function as a bacterial adhesin. In conclusion, LppA emerges as a novel adhesion protein that interacts with multiple host extracellular matrix proteins and ANXA2, playing a crucial role in M. bovis’s adherence to host cells and dissemination. These insights substantially deepen our comprehension of the molecular pathogenesis of M. bovis.
Prehabilitation programs for individuals with cancer: a systematic review of randomized-controlled trials
Authors: Meneses-Echavez Jose F. , Loaiza-Betancur Andrés F. , Díaz-López Víctor , Echavarría-Rodríguez Andrés M. , Triana-Reina Héctor Reynaldo
Published: November 2023

Background

Prehabilitation programs focusing on exercise training as the main component are known as a promising alternative for improving patients’ outcomes before cancer surgery. This systematic review determined the benefits and harms of prehabilitation programs compared with usual care for individuals with cancer.

Methods

We searched CENTRAL, MEDLINE, and EMBASE from inception to June 2022, and hand searched clinical trial registries. We included randomized-controlled trials (RCTs) in adults, survivors of any type of cancer, that compared prehabilitation programs that had exercise training as the major component with usual care or other active interventions. Outcome measures were health-related quality of life (HRQL), muscular strength, postoperative complications, average length of stay (ALOS), handgrip strength, and physical activity levels. Two reviewers independently screened the studies, extracted data, and assessed the risk of bias and the certainty of the evidence.

Results

Twenty-five RCTs (2682 participants) published between 2010 and 2022 met our inclusion criteria. Colorectal and lung cancers were the most common diagnoses. The studies had methodological concerns regarding outcome measurement, selective reporting, and attrition. Five prehabilitation programs were compared to usual care (rehabilitation): combined training, aerobic training, respiratory muscle training plus aerobic training, respiratory muscle training plus resistance training, and pelvic floor training. The studies provided no clear evidence of an effect between groups. We assessed the overall certainty of the evidence as very low, downgraded due to serious study limitations and imprecision.

Conclusion

Prehabilitation programs focusing on exercise training may have an effect on adults with cancer, but the evidence is very uncertain. We have very little confidence in the results and the true effect is likely to be substantially different from these. Further research is needed before we can draw a more certain conclusion.

Systematic review registration

CRD42019125658.
Re-estimation of the savings retention coefficient in OECD countries: a new measure of home country bias
Authors: Takahashi Harutaka
Published: November 2023

The recent home bias is primarily related to the equity bias as measured by the international capital asset pricing model (CAPM); this measure has declined over the past two decades amid financial globalization, but remains high in most developed countries. The key to understanding this puzzling phenomenon lies in how accurately this indicator can be predicted. In this paper, we propose to use the savings retention coefficient estimated by the Feldstein–Horioka (F–H) regression as a cross-country measure of home bias. We re-estimate the savings retention coefficient based on an estimation model in which the FH regression is embedded in the model derived from Tobin's q-theory of saddle-road dynamics of investment under convex adjustment costs. We then use dynamic panel estimation to estimate the new measure in OECD countries. The main empirical results are as follows. The new measure of home country bias, such as the equity bias measure, declined steadily until 2008, but recovered to the level of the 60 s and 70 s after the 2008 financial crisis. Interestingly, people expected home country bias to be very high after the financial crisis, but in fact it simply returned to its previous level.
Medium-term interactive effects of herbivores and plant life form on the biochemistry of shallow sandy soils in a protected semi-arid savanna
Authors: Malongweni Siviwe Odwa , van Tol Johan
Published: November 2023

Savannas are characterized by the co-occurrence of two different plant life forms: grasses and trees. Herbivory plays a major role in the balance between grasses and trees in savanna ecosystems. The present study aimed to investigate the impact and interactions between long-term (i.e. 20 years) herbivory and/or its exclusion and plant life form on the soil biochemistry of a protected semi-arid savanna ecosystem in the Kruger National Park (KNP), South Africa. To study the effects of herbivory on soil properties, herbivore exclosures (fully fenced areas, partially fenced areas, and an unfenced area) were used in conjunction with plant life form (trees and grasses) were considered. Interaction effects of herbivory and plant life form on soil pH, electrical conductivity (EC), total nitrogen (TN), total carbon (TC), available phosphorus (available P), exchangeable cations (K+, Na+, Mg2+and Ca2+) cation exchange capacity (CEC), organic matter (OM) and total microbial activity were determined on savanna soils in the Nkuhlu exclosures, KNP. Exclosures where herbivores were present had significantly higher soil pH, The presence of herbivores caused an increase in soil pH, EC, exchangeable Na, CEC, and OM. The influence of the tree canopy was significantly more pronounced in elevating total C and N, exchangeable K+, Mg2+and Ca2+, CEC and OM than observed in the open grassland zones across all exclosures. The two-way interaction between herbivory and plant life form resulted in significant decreases in TN, TC, exchangeable K, Na and Mg in open grassland areas outside of herbivore exclosures where large animals had direct access, as compared to areas within the exclosures which was protected from animal entry herbivory. This data can be used by national parks as an indicator to increase their knowledge of environmental issues relating to maintaining and preserving landscape features of savannas.
Optimal distribution of VLBI transmitters in the Galileo space segment for frame ties
Authors: Wolf Helene , Böhm Johannes
Published: November 2023

Equipping Galileo satellites with a VLBI transmitter (VT) will allow to observe satellites next to quasars with Very Long Baseline Interferometry (VLBI) radio telescopes. This concept will facilitate the direct estimation of the satellite orbits in the celestial reference frame. Moreover, these observations along with usual Galileo observations can be used to transfer the space tie between the VT and the antenna on the Galileo satellite to the Earth surface realizing the frame tie at the geodetic site with VLBI radio telescope and Galileo antenna. In this study, we assess the accuracy of that frame tie by simulating the estimation of station coordinates from VLBI observations to Galileo satellites next to quasars. We find that at least two or three satellites need to be equipped with a VT with the best results if all satellites with a VT are placed in the same plane. Concerning the ratio between satellite and quasar observations within a schedule, the results suggest that the optimal ratio is around 30% to 40% satellite observations out of the total number of observations in order to have enough observations for the estimation of the station coordinates but still enough quasar observations to ensure a sufficient sky-coverage for the estimation of troposphere parameters. The best scenario with two satellites yields repeatabilities for the east and north components between 7.5 and 10 mm, and for the up component between 9.5 and 12 mm. In case there is a third satellite with a VLBI transmitter in the same plane, the repeatabilities are reduced by up to 2 mm for the horizontal components and up to 3 to 4 mm for the up component. Rotating the schedules over the constellation repeat cycle of Galileo of 10 days reveals that there are differences between the individual days, but there are no days with a significantly worse precision of the estimated station coordinates.

Graphical Abstract
Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study
Authors: Popal Zohal , Nickel Katrin F. , Wöltje Michael , Aibibu Dilbar , Knipfer Christian , Smeets Ralf , Renné Thomas
Published: November 2023

Purpose

Post-interventional hemorrhage can result in serious complications, especially in patients with hemostatic disorders. Identification of safe and efficient local hemostatic agents is important, particularly in the context of an ageing society and the emergence of new oral anticoagulants. The aim of this in vitro study was to investigate the potential of silk fibroin membranes coated with the inorganic polymer polyphosphate (polyP) as a novel hemostatic device in oral surgery.

Methods

Cocoons of the silkworm Bombyx mori were degummed and dissolved. Varying amounts of long-chain polyP (2–2000 µg/mm2) were adsorbed to the surface of silk fibroin membranes. Analysis of the procoagulant effect of polyP-coated silk membranes was performed using real-time thrombin generation assays in human plasma. Increasing concentrations of polyP (0.15–500 µg/ml) served as a positive control, while uncoated silk fibroin membranes were used as negative control.

Results

PolyP-coated silk fibroin membranes triggered coagulation when compared to plasma samples and pure silk fibroin membranes. A polyP-dose-dependent effect of thrombin generation could be found with a maximum (ETP = 1525.7 nM⋅min, peak thrombin = 310.1 nM, time to peak = 9.8 min, lag time = 7.6 min.) at 200 µg/mm2 of polymer loading on the silk fibroin membrane surface.

Conclusions

In this study, it was demonstrated that silk fibroin membranes coated with polyP have the potential to act as a promising novel hemostatic device.

Graphical Abstract
Cognitive distortions and perfectionism during bipolar and unipolar depressive episode
Authors: Aral Ayşe Ergüner , Sarisoy Gökhan , Aral Armagan
Published: November 2023

Background

The aim of the current study was to compare bipolar depression (BD) and unipolar depression (UD) patients and healthy controls in terms of their cognitive distortions and perfectionist traits during acute depressive episodes.

Results

The current dissertation study is a cross-sectional study consisting of 80 patients with unipolar depression, 80 patients with bipolar depression, and 80 healthy controls. Sociodemographic and Clinical Data Form, Cognitive Distortion Scale (CDS), Frost’s Multidimensional Perfectionism Scale (FMPS), Hamilton Anxiety Rating Scale (HARS), Hamilton Depression Rating Scale (HDRS), and Young Mania Rating Scale (YMRS) were used as measurement tools. Healthy controls were selected on the basis that they had the same gender and age distribution as the other participants. It was found that patients with unipolar and bipolar depression had statistically equal but higher total CDS interpersonal and personal achievement scores than healthy controls, and all subtypes of the CDS labeling score were higher in the unipolar depression group than in the other groups. It was found that both depression groups had higher FMPS self-oriented and social-oriented perfectionism scores than healthy controls, lower others-oriented perfectionism scores than healthy controls, and there were no statistically significant differences between them in terms of perfectionism subtypes.

Conclusions

In the light of the research data, it was found that the patients with bipolar/unipolar depression had more cognitive distortion than healthy controls and cognitive distortion was not significantly different in the two groups. According to the research data, it is observed that perfectionism is higher in unipolar depression than in bipolar depression. The data obtained have the potential to provide a theoretical basis for a psychotherapeutic approach.
Erectile dysfunction: role of computed tomography cavernosography in the diagnosis and treatment planning of venous leak
Authors: Hoppe Hanno , Hirschle Dominique , Schumacher Martin Christian , Schönhofen Heinz , Glenck Michael , Kalka Christoph , Willenberg Torsten , Sixt Sebastian , Müller Dominik , Gutzeit Andreas , Christe Andreas , Mohan Vignes , Diehm Nicolas
Published: November 2023

Background

Venous leak appears to be the most common cause of vasculogenic erectile dysfunction (ED), which can be treated with venous embolization. Traditionally, conventional cavernosography was used for the diagnosis and treatment planning of venous leak. Recently, computed tomography (CT) cavernosography was introduced as a novel cross-sectional imaging method proposed to be advantageous over conventional cavernosography. We created a novel management algorithm for diagnosing venous leak including CT cavernosography as an imaging modality. In order to provide a broader basis for our management algorithm, a systematic literature review was conducted.

Main body

In this article we systematically review relevant literature on using CT cavernosography for the diagnosis and treatment planning in ED patients with venous leak following the PRISMA selection process. Nine full-text articles were included in the review and assigned a level of evidence grade (all grade II). Two studies (2/9) compared the results of conventional cavernosography with those of CT cavernosography which was superior for site-specific venous leak identification (19.4% vs. 100%, respectively). CT cavernosography is a more detailed imaging method that is faster to perform, exposes the patient to less radiation, and requires less contrast material. In one study (1/9), CT cavernosography was used for diagnostic purposes only. Eight studies (8/9) cover both, diagnostic imaging and treatment planning including embolization (1/9) and sclerotherapy (2/9) of venous leak in patients with venogenic ED. Three studies (3/9) describe anatomical venous leak classifications that were established based on CT cavernosography findings for accurate mapping of superficial and/or deep venous leak and identification of mixed or more complex forms of venous leak present in up to 84% of patients. In addition to treatment planning, one study (1/9) used CT cavernosography also for follow-up imaging post treatment.

Conclusion

CT cavernosography is superior to conventional cavernosography for diagnosis and treatment planning in patients with ED caused by venous leak (grade II levels of evidence). Consequently, CT cavernosography should be included in management algorithms for ED patients with suspected venous leak.
B-lymphocyte-activating factor is a potential biomarker associated with susceptibility to Graves’ disease in Iraqi women
Authors: Ibrahim Hiba Y. , Sulaiman Ghassan M. , Ad’hiah Ali H. , Al-shammaa Mohamed S.
Published: November 2023

Background

B-lymphocyte-activating factor (BAFF) is a cytokine involved in regulating the development and maturation of B lymphocyte and has been shown to be up-regulated in patients with Graves’ disease (GD). However, the association of TNFSF13B variants (the gene that encodes BAFF) with the risk of GD has not been well explored. In this case–control study, the aim was to evaluate the role of BAFF, in terms of serum level and polymorphism, in the etio-pathogenesis of GD. Therefore, serum BAFF concentrations were analyzed in Iraqi women with GD and age-matched control women (n = 90 and 93, respectively) using an ELISA kit. In addition, two promoter variants of the TNFSF13B gene, rs9514827 (T > C) and rs9514828 (C > T), were genotyped using a PCR–RFLP-based assay.

Results

Median BAFF concentrations (interquartile range) were significantly elevated in GD patients compared to controls (1525 [1327–1840] vs. 689 [585–807] pg/mL; probability [p] < 0.001). Elevated BAFF concentrations were a reliable predictor of GD as indicated by the area under the curve of 0.971. BAFF was positively correlated with triiodothyronine (correlation coefficient [rs] = 0.216; p = 0.041) and thyroxine (rs = 0.269; p = 0.01) in GD patients. Mutant alleles, rs9514827 C (odds ratio [OR] = 2.00; p = 0.008; corrected p [pc] = 0.048) and rs9514828 T (OR = 2.15; p = 0.002; pc = 0.012), as well as genotypes, rs9514827 CC (OR = 4.29; p = 0.032; pc = 0.192) and rs9514828 TT (OR = 4.57; p = 0.003; pc = 0.018), were associated with a greater risk of GD. Besides, the C-T haplotype (rs9514827-rs9514828) was also linked to an elevated risk of GD among Iraqi women (OR = 2.71; p = 0.006; pc = 0.024).

Conclusions

BAFF showed up-regulated levels in the serum of women with GD. In light of this, BAFF has been proposed as a reliable prognostic biomarker for GD. Regarding its relationship to thyroid hormones, BAFF showed a positive correlation with triiodothyronine and thyroxine. Both variants (rs9514827 and rs9514828) of the TNFSF13B gene showed an association with susceptibility to GD, and rs9514828 may have up-regulatory effects on BAFF levels.