bluuebunny/crossref_metadata_2025 huggingface dataset:
Myricetin Attenuates Depressant-Like Behavior in Mice Subjected to Repeated Restraint Stress
Authors: Ma Zegang , Wang Guilin , Cui Lin , Wang Qimin
Published: November 2015

Increasing evidence has shown that oxidative stress may be implicated in chronic stress-induced depression. Several flavonoids with anti-oxidative effects have been proved to be anti-depressive. Myricetin is a well-defined flavonoid with the anti-oxidative, anti-inflammatory, anti-apoptotic, and neuroprotective properties. The aim of the present study is to investigate the possible effects of chronic administration of myricetin on depressant-like behaviors in mice subjected to repeated restraint (4 h/day) for 21 days. Our results showed that myricetin administration specifically reduced the immobility time in mice exposed to chronic stress, as tested in both forced swimming test and tail suspension test. Myricetin treatment improved activities of glutathione peroxidase (GSH-PX) in the hippocampus of stressed mice. In addition, myricetin treatment decreased plasma corticosterone levels of those mice subjected to repeated restraint stress. The effects of myricetin on the brain-derived neurotrophic factor (BDNF) levels in hippocampus were also investigated. The results revealed that myricetin normalized the decreased BDNF levels in mice subjected to repeated restraint stress. These findings provided more evidence that chronic administration of myricetin improves helpless behaviors. The protective effects of myricetin might be partially mediated by an influence on BDNF levels and might be attributed to myricetin-mediated anti-oxidative stress in the hippocampus.
Thrombophilia and Pregnancy Complications
Authors: Simcox Louise , Ormesher Laura , Tower Clare , Greer Ian
Published: November 2015

There is a paucity of strong evidence associated with adverse pregnancy outcomes and thrombophilia in pregnancy. These problems include both early (recurrent miscarriage) and late placental vascular-mediated problems (fetal loss, pre-eclampsia, placental abruption and intra-uterine growth restriction). Due to poor quality case-control and cohort study designs, there is often an increase in the relative risk of these complications associated with thrombophilia, particularly recurrent early pregnancy loss, late fetal loss and pre-eclampsia, but the absolute risk remains very small. It appears that low-molecular weight heparin has other benefits on the placental vascular system besides its anticoagulant properties. Its use is in the context of antiphospholipid syndrome and recurrent pregnancy loss and also in women with implantation failure to improve live birth rates. There is currently no role for low-molecular weight heparin to prevent late placental-mediated complications in patients with inherited thrombophilia and this may be due to small patient numbers in the studies involved in summarising the evidence. There is potential for low-molecular weight heparin to improve pregnancy outcomes in women with prior severe vascular complications of pregnancy such as early-onset intra-uterine growth restriction and pre-eclampsia but further high quality randomised controlled trials are required to answer this question.
Claudin 1 in Breast Cancer: New Insights
Authors: Zhou Bowen , Moodie Amanda , Blanchard Anne , Leygue Etienne , Myal Yvonne
Published: November 2015

Claudin 1 is a small transmembrane protein responsible for maintaining the barrier function that exists between epithelial cells. A tight junction protein that regulates the paracellular transport of small ions across adjacent cells, claudin 1 maintains cellular polarity and plays a major role in cell-cell communication and epithelial cell homeostasis. Long considered to be a putative tumor suppressor in human breast cancer, new studies suggest a role much more complex. While most invasive breast cancers exhibit a down regulation or absence of claudin 1, some aggressive subtypes that exhibit high claudin 1 levels have now been described. Furthermore, a causal role for claudin 1 in breast cancer progression has recently been demonstrated in some breast cancer cell lines. In this review we highlight new insights into the role of claudin 1 in breast cancer, including its involvement in collective migration and epithelial mesenchymal transition (EMT).
Influence of Hot-Etching Surface Treatment on Zirconia/Resin Shear Bond Strength
Authors: Lv Pin , Yang Xin , Jiang Ting
Published: November 2015

This study was designed to evaluate the effect of hot-etching surface treatment on the shear bond strength between zirconia ceramics and two commercial resin cements. Ceramic cylinders (120 units; length: 2.5 mm; diameter: 4.7 mm) were randomly divided into 12 groups (n = 10) according to different surface treatments (blank control; airborne-particle-abrasion; hot-etching) and different resin cements (Panavia F2.0; Superbond C and B) and whether or not a thermal cycling fatigue test (5°–55° for 5000 cycles) was performed. Flat enamel surfaces, mounted in acrylic resin, were bonded to the zirconia discs (diameter: 4.7 mm). All specimens were subjected to shear bond strength testing using a universal testing machine with a crosshead speed of 1 mm/min. All data were statistically analyzed using one-way analysis of variance and multiple-comparison least significant difference tests (α = 0.05). Hot-etching treatment produced higher bond strengths than the other treatment with both resin cements. The shear bond strength of all groups significantly decreased after the thermal cycling test; except for the hot-etching group that was cemented with Panavia F2.0 (p < 0.05). Surface treatment of zirconia with hot-etching solution enhanced the surface roughness and bond strength between the zirconia and the resin cement.
In Situ High-Energy X-ray Diffraction during Hot-Forming of a Multiphase TiAl Alloy
Authors: Stark Andreas , Rackel Marcus , Tchouaha Tankoua Aristide , Oehring Michael , Schell Norbert , Lottermoser Lars , Schreyer Andreas , Pyczak Florian
Published: November 2015

Intermetallic γ-TiAl based alloys exhibit excellent high-temperature strength combined with low density. This makes them ideal candidates for replacing the twice as dense Ni base super-alloys, currently used in the medium temperature range (~700 °C) of industrial and aviation gas turbines. An important step towards the serial production of TiAl parts is the development of suitable hot-forming processes. Thermo-mechanical treatments often result in mechanical anisotropy due to the formation of crystallographic textures. However, with conventional texture analysis techniques, their formation can only be studied after processing. In this study, in situ high-energy X-ray diffraction measurements with synchrotron radiation were performed during hot-forming. Thus, it was possible to record the evolution of the phase constitution as well as the formation of crystallographic texture of different phases directly during processing. Several process temperatures (1100 °C to 1300 °C) and deformation rates were investigated. Based on these experiments, a process window can be recommended which results in the formation of an optimal reduced texture.
An Inert Continuous Microreactor for the Isolation and Analysis of a Single Microbial Cell
Authors: Rosenthal Katrin , Falke Floris , Frick Oliver , Dusny Christian , Schmid Andreas
Published: November 2015

Studying biological phenomena of individual cells is enabled by matching the scales of microbes and cultivation devices. We present a versatile, chemically inert microfluidic lab-on-a-chip (LOC) device for biological and chemical analyses of isolated microorganisms. It is based on the Envirostat concept and guarantees constant environmental conditions. A new manufacturing process for direct fusion bonding chips with functional microelectrodes for selective and gentle cell manipulation via negative dielectrophoresis (nDEP) was generated. The resulting LOC system offered a defined surface chemistry and exceptional operational stability, maintaining its structural integrity even after harsh chemical treatment. The microelectrode structures remained fully functional after thermal bonding and were proven to be efficient for single-cell trapping via nDEP. The microfluidic network consisted solely of glass, which led to enhanced chip reusability and minimized interaction of the material with chemical and biological compounds. We validated the LOC for single-cell studies with the amino acid secreting bacterium Corynebacterium glutamicum. Intracellular l-lysine production dynamics of individual bacteria were monitored based on a genetically encoded fluorescent nanosensor. The results demonstrate the applicability of the presented LOC for pioneering chemical and biological studies, where robustness and chemically inert surfaces are crucial parameters for approaching fundamental biological questions at a single-cell level.
Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Authors: Zhao Yue-Mei , Zhou Tao , Li Zhong-Hu , Zhao Gui-Fang
Published: November 2015

Gynostemma pentaphyllum is an important medicinal herb of the Cucurbitaceae family, but limited genomic data have hindered genetic studies. In this study, transcriptomes of two closely-related Gynostemma species, Gynostemma cardiospermum and G. pentaphyllum, were sequenced using Illumina paired-end sequencing technology. A total of 71,607 nonredundant unigenes were assembled. Of these unigenes, 60.45% (43,288) were annotated based on sequence similarity search with known proteins. A total of 11,059 unigenes were identified in the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG) database. A total of 3891 simple sequence repeats (SSRs) were detected in 3526 nonredundant unigenes, 2596 primer pairs were designed and 360 of them were randomly selected for validation. Of these, 268 primer pairs yielded clear products among six G. pentaphyllum samples. Thirty polymorphic SSR markers were used to test polymorphism and transferability in Gynostemma. Finally, 15 SSR makers that amplified in all 12 Gynostemma species were used to assess genetic diversity. Our results generated a comprehensive sequence resource for Gynostemma research.
Factors Influencing Oral Bioavailability of Thai Mango Seed Kernel Extract and Its Key Phenolic Principles
Authors: Jiamboonsri Pimsumon , Pithayanukul Pimolpan , Bavovada Rapepol , Leanpolchareanchai Jiraporn , Yin Taijun , Gao Song , Hu Ming
Published: November 2015

Mango seed kernel extract (MSKE) and its key components (gallic acid, GA; methyl gallate, MG; and pentagalloyl glucopyranose, PGG) have generated interest because of their pharmacological activities. To develop the potential use of the key components in MSKE as natural therapeutic agents, their pharmacokinetic data are necessary. Therefore, this study was performed to evaluate the factors affecting their oral bioavailability as pure compounds and as components in MSKE. The in vitro chemical stability, biological stability, and absorption were evaluated in Hanks’ Balanced Salt Solution, Caco-2 cell and rat fecal lysates, and the Caco-2 cell model, respectively. The in vivo oral pharmacokinetic behavior was elucidated in Sprague-Dawley rats. The key components were unstable under alkaline conditions and in Caco-2 cell lysates or rat fecal lysates. The absorptive permeability coefficient followed the order MG > GA > PGG. The in vivo results exhibited similar pharmacokinetic trends to the in vitro studies. Additionally, the co-components in MSKE may affect the pharmacokinetic behaviors of the key components in MSKE. In conclusion, chemical degradation under alkaline conditions, biological degradation by intestinal cell and colonic microflora enzymes, and low absorptive permeability could be important factors underlying the oral bioavailability of these polyphenols.
Metabolic Characteristics in Meal of Black Rapeseed and Yellow-Seeded Progeny of Brassica napus–Sinapis alba Hybrids
Authors: Jiang Jinjin , Wang Yue , Xie Tao , Rong Hao , Li Aimin , Fang Yujie , Wang Youping
Published: November 2015

Breeding of yellow-seeded rapeseed (Brassica napus) is preferred over black-seeded rapeseed for the desirable properties of the former. This study evaluated the metabolites and nutritive values of black-seeded rapeseed meal and yellow-seeded meal from the progeny of a B. napus–Sinapis alba hybrid. Yellow-seed meal presented higher protein (35.46% vs. 30.29%), higher sucrose (7.85% vs. 7.29%), less dietary fiber (26.19% vs. 34.63%) and crude fiber (4.56% vs. 8.86%), and less glucosinolates (22.18 vs. 28.19 μmol/g) than black-seeded one. Amounts of ash (3.65% vs. 4.55%), phytic acid (4.98% vs. 5.60%), and total polyphenols (2.67% vs. 2.82%) were decreased slightly in yellow-seeded meal compared with black-seeded meal. Yellow-seeded meal contained more essential amino acids than black-seeded meal. Levels of the mineral elements Fe, Mn, and Zn in yellow-seeded meal were higher than black-seeded meal. By contrast, levels of P, Ca, and Mg were lower in yellow-seeded meal. Moreover, yellow-seeded meal showed lower flavonol (kaempferol, quercetin, isorhamnetin, and their derivatives) content than black-seeded meal. Comparison of metabolites between yellow and black rapeseed confirmed the improved nutritional value of meal from yellow-seeded B. napus, and this would be helpful to the breeding and improvement of rapeseed for animal feeding.
Antisense Activity across the Nesp Promoter is Required for Nespas-Mediated Silencing in the Imprinted Gnas Cluster
Authors: Tibbit Charlotte , Williamson Christine , Mehta Stuti , Ball Simon , Chotalia Mita , Nottingham Wade , Eaton Sally , Quwailid Mohamed , Teboul Lydia , Kelsey Gavin , Peters Jo
Published: November 2015

Macro long non-coding RNAs (lncRNAs) play major roles in gene silencing in inprinted gene clusters. Within the imprinted Gnas cluster, the paternally expressed Nespas lncRNA downregulates its sense counterpart Nesp. To explore the mechanism of action of Nespas, we generated two new knock-in alleles to truncate Nespas upstream and downstream of the Nesp promoter. We show that Nespas is essential for methylation of the Nesp differentially methylated region (DMR), but higher levels of Nespas are required for methylation than are needed for downregulation of Nesp. Although Nespas is transcribed for over 27 kb, only Nespas transcript/transcription across a 2.6 kb region that includes the Nesp promoter is necessary for methylation of the Nesp DMR. In both mutants, the levels of Nespas were extraordinarily high, due at least in part to increased stability, an effect not seen with other imprinted lncRNAs. However, even when levels were greatly raised, Nespas remained exclusively cis-acting. We propose Nespas regulates Nesp methylation and expression to ensure appropriate levels of expression of the protein coding transcripts Gnasxl and Gnas on the paternal chromosome. Thus, Nespas mediates paternal gene expression over the entire Gnas cluster via a single gene, Nesp.
Prompt Proxy Mapping of Flood Damaged Rice Fields Using MODIS-Derived Indices
Authors: Kwak Youngjoo , Arifuzzanman Bhuyan , Iwami Yoichi
Published: November 2015

Flood mapping, particularly hazard and risk mapping, is an imperative process and a fundamental part of emergency response and risk management. This paper aims to produce a flood risk proxy map of damaged rice fields over the whole of Bangladesh, where monsoon river floods are dominant and frequent, affecting over 80% of the total population. This proxy risk map was developed to meet the request of the government on a national level. This study represents a rapid, straightforward methodology for estimating rice-crop damage in flood areas of Bangladesh during the large flood from July to September 2007, despite the lack of primary data. We improved a water detection algorithm to achieve a better discrimination capacity to discern flood areas by using a modified land surface water index (MLSWI). Then, rice fields were estimated utilizing a hybrid rice field map from land-cover classification and MODIS-derived indices, such as the normalized difference vegetation index (NDVI) and enhanced vegetation index (EVI). The results showed that the developed method is capable of providing instant, comprehensive, nationwide mapping of flood risks, such as rice field damage. The detected flood areas and damaged rice fields during the 2007 flood were verified by comparing them with the Advanced Land Observing Satellite (ALOS) AVNIR-2 images (a 10 m spatial resolution) and in situ field survey data with moderate agreement (K = 0.57).
InSAR Time Series Analysis of Natural and Anthropogenic Coastal Plain Subsidence: The Case of Sibari (Southern Italy)
Authors: Cianflone Giuseppe , Tolomei Cristiano , Brunori Carlo , Dominici Rocco
Published: November 2015

We applied the Small Baseline Subset multi-temporal InSAR technique (SBAS) to two SAR datasets acquired from 2003 up to 2013 by Envisat (ESA, European Space Agency) and COSMO-SkyMed (ASI, Italian Space Agency) satellites to investigate spatial and temporal patterns of land subsidence in the Sibari Plain (Southern Italy). Subsidence processes (up to ~20 mm/yr) were investigated comparing geological, hydrogeological, and land use information with interferometric results. We suppose a correlation between subsidence and thickness of the Plio-Quaternary succession suggesting an active role of the isostatic compensation. Furthermore, the active back thrusting in the Corigliano Gulf could trigger a flexural subsidence mechanism even if fault activity and earthquakes do not seem play a role in the present subsidence. In this context, the compaction of Holocene deposits contributes to ground deformation. Despite the rapid urbanization of the area in the last 50 years, we do not consider the intensive groundwater pumping and related water table drop as the main triggering cause of subsidence phenomena, in disagreement with some previous publications. Our interpretation for the deformation fields related to natural and anthropogenic factors would be a comprehensive and exhaustive justification to the complexity of subsidence processes in the Sibari Plain.
Downscaling Surface Water Inundation from Coarse Data to Fine-Scale Resolution: Methodology and Accuracy Assessment
Authors: Wu Guiping , Liu Yuanbo
Published: November 2015

The availability of water surface inundation with high spatial resolution is of fundamental importance in several applications such as hydrology, meteorology and ecology. Medium spatial resolution sensors, like MODerate-resolution Imaging Spectroradiometer (MODIS), exhibit a significant potential to study inundation dynamics over large areas because of their high temporal resolution. However, the low spatial resolution provided by MODIS is not appropriate to accurately delineate inundation over small scale. Successful downscaling of water inundation from coarse to fine resolution would be crucial for improving our understanding of complex inundation characteristics over the regional scale. Therefore, in this study, we propose an innovative downscaling method based on the normalized difference water index (NDWI) statistical regression algorithm towards generating small-scale resolution inundation maps from MODIS data. The method was then applied to the Poyang Lake of China. To evaluate the performance of the proposed downscaling method, qualitative and quantitative comparisons were conducted between the inundation extent of MODIS (250 m), Landsat (30 m) and downscaled MODIS (30 m). The results indicated that the downscaled MODIS (30 m) inundation showed significant improvement over the original MODIS observations when compared with simultaneous Landsat (30 m) inundation. The edges of the lakes become smoother than the results from original MODIS image and some undetected water bodies were delineated with clearer shapes in the downscaled MODIS (30 m) inundation map. With respect to high-resolution Landsat TM/ETM+ derived inundation, the downscaling procedure has significantly increased the R2 and reduced RMSE and MAE both for the inundation area and for the value of landscape metrics. The main conclusion of this study is that the downscaling algorithm is promising and quite feasible for the inundation mapping over small-scale lakes.
Precise Positioning of BDS, BDS/GPS: Implications for Tsunami Early Warning in South China Sea
Authors: Chen Kejie , Zamora Natalia , Babeyko Andrey , Li Xingxing , Ge Maorong
Published: November 2015

Global Positioning System (GPS) has been proved to be a powerful tool for measuring co-seismic ground displacements with an application to seismic source inversion. Whereas most of the tsunamis are triggered by large earthquakes, GPS can contribute to the tsunami early warning system (TEWS) by helping to obtain tsunami source parameters in near real-time. Toward the end of 2012, the second phase of the BeiDou Navigation Satellite System (BDS) constellation was accomplished, and BDS has been providing regional positioning service since then. Numerical results indicate that precision of BDS nowadays is equivalent to that of the GPS. Compared with a single Global Satellite Navigation System (GNSS), combined BDS/GPS real-time processing can improve accuracy and especially reliability of retrieved co-seismic displacements. In the present study, we investigate the potential of BDS to serve for the early warning system of tsunamis in the South China Sea region. To facilitate early warnings of tsunamis and forecasting capabilities in this region, we propose to distribute an array of BDS-stations along the Luzon Island (Philippines). By simulating an earthquake with Mw = 8 at the Manila trench as an example, we demonstrate that such an array will be able to detect earthquake parameters in real time with a high degree of accuracy and, hence, contribute to the fast and reliable tsunami early warning system in this region.
Process Mining Methodology for Health Process Tracking Using Real-Time Indoor Location Systems
Authors: Fernandez-Llatas Carlos , Lizondo Aroa , Monton Eduardo , Benedi Jose-Miguel , Traver Vicente
Published: November 2015

The definition of efficient and accurate health processes in hospitals is crucial for ensuring an adequate quality of service. Knowing and improving the behavior of the surgical processes in a hospital can improve the number of patients that can be operated on using the same resources. However, the measure of this process is usually made in an obtrusive way, forcing nurses to get information and time data, affecting the proper process and generating inaccurate data due to human errors during the stressful journey of health staff in the operating theater. The use of indoor location systems can take time information about the process in an unobtrusive way, freeing nurses, allowing them to engage in purely welfare work. However, it is necessary to present these data in a understandable way for health professionals, who cannot deal with large amounts of historical localization log data. The use of process mining techniques can deal with this problem, offering an easily understandable view of the process. In this paper, we present a tool and a process mining-based methodology that, using indoor location systems, enables health staff not only to represent the process, but to know precise information about the deployment of the process in an unobtrusive and transparent way. We have successfully tested this tool in a real surgical area with 3613 patients during February, March and April of 2015.
Electromagnetic Modelling of Fiber Sensors for Low-Cost and High Sensitivity Temperature Monitoring
Authors: Scarcia William , Palma Giuseppe , Falconi Mario , De Leonardis Francesco , Passaro Vittorio , Prudenzano Francesco
Published: November 2015

An accurate design of an innovative fiber optic temperature sensor is developed. The sensor is based on a cascade of three microstructured optical fibers (MOFs). In the first one a suitable cascade of long period gratings is designed into the core. A single mode intermediate and a rare-earth activated Fabry-Perot optical cavity are the other two sensor MOF sections. An exhaustive theoretic feasibility investigation is performed employing computer code. The complete set-up for temperature monitoring can be obtained by utilizing only a low cost pump diode laser at 980 nm wavelength and a commercial optical power detector. The simulated sensitivity S = 315.1 μW/°C and the operation range ΔT = 100 °C is good enough for actual applications.
Temperature Effects on the Wind Direction Measurement of 2D Solid Thermal Wind Sensors
Authors: Chen Bei , Zhu Yan-Qing , Yi Zhenxiang , Qin Ming , Huang Qing-An
Published: November 2015

For a two-dimensional solid silicon thermal wind sensor with symmetrical structure, the wind speed and direction information can be derived from the output voltages in two orthogonal directions, i.e., the north-south and east-west. However, the output voltages in these two directions will vary linearly with the ambient temperature. Therefore, in this paper, a temperature model to study the temperature effect on the wind direction measurement has been developed. A theoretical analysis has been presented first, and then Finite Element Method (FEM) simulations have been performed. It is found that due to symmetrical structure of the thermal wind sensor, the temperature effects on the output signals in the north-south and east-west directions are highly similar. As a result, the wind direction measurement of the thermal wind sensor is approximately independent of the ambient temperature. The experimental results fit the theoretical analysis and simulation results very well.
Identification of Chinese Herbs Using a Sequencing-Free Nanostructured Electrochemical DNA Biosensor
Authors: Lei Yan , Yang Fan , Tang Lina , Chen Keli , Zhang Guo-Jun
Published: November 2015

Due to the nearly identical phenotypes and chemical constituents, it is often very challenging to accurately differentiate diverse species of a Chinese herbal genus. Although technologies including DNA barcoding have been introduced to help address this problem, they are generally time-consuming and require expensive sequencing. Herein, we present a simple sequencing-free electrochemical biosensor, which enables easy differentiation between two closely related Fritillaria species. To improve its differentiation capability using trace amounts of DNA sample available from herbal extracts, a stepwise electrochemical deposition of reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) was adopted to engineer a synergistic nanostructured sensing interface. By using such a nanofeatured electrochemical DNA (E-DNA) biosensor, two Chinese herbal species of Fritillaria (F. thunbergii and F. cirrhosa) were successfully discriminated at the DNA level, because a fragment of 16-mer sequence at the spacer region of the 5S-rRNA only exists in F. thunbergii. This E-DNA sensor was capable of identifying the target sequence in the range from 100 fM to 10 nM, and a detection limit as low as 11.7 fM (S/N = 3) was obtained. Importantly, this sensor was applied to detect the unique fragment of the PCR products amplified from F. thunbergii and F. cirrhosa, respectively. We anticipate that such a direct, sequencing-free sensing mode will ultimately pave the way towards a new generation of herb-identification strategies.
A Single Eu-Doped In2O3 Nanobelt Device for Selective H2S Detection
Authors: Chen Weiwu , Liu Yingkai , Qin Zhaojun , Wu Yuemei , Li Shuanghui , Ai Peng
Published: November 2015

Eu-doped In2O3 nanobelts (Eu-In2O3 NBs) and pure In2O3 nanobelts (In2O3 NBs) are synthesized by the carbon thermal reduction method. Single nanobelt sensors are fabricated via an ion beam deposition system with a mesh-grid mask. The gas-sensing response properties of the Eu-In2O3 NB device and its undoped counterpart are investigated with several kinds of gases (including H2S, CO, NO2, HCHO, and C2H5OH) at different concentrations and different temperatures. It is found that the response of the Eu-In2O3 NB device to 100 ppm of H2S is the best among these gases and the sensitivity reaches 5.74, which is five times that of pure In2O3 NB at 260 °C. We also found that the former has an excellent sensitive response and great selectivity to H2S compared to the latter. Besides, there is a linear relationship between the response and H2S concentration when its concentration changes from 5 to 100 ppm and from 100 to 1000 ppm. The response/recovery time is quite short and remains stable with an increase of H2S concentration. These results mean that the doping of Eu can improve the gas-sensing performance of In2O3 NB effectually.
Identifying Challenges to Building an Evidence Base for Restoration Practice
Authors: Ntshotsho Phumza , Esler Karen , Reyers Belinda
Published: November 2015

Global acknowledgement of ecological restoration, as an important tool to complement conservation efforts, requires an effort to increase the effectiveness of restoration interventions. Evidence-based practice is purported to promote effectiveness. A central tenet of this approach is decision making that is based on evidence, not intuition. Evidence can be generated experimentally and in practice but needs to be linked to baseline information collection, clear goals and monitoring of impact. In this paper, we report on a survey conducted to assess practitioners’ perceptions of the evidence generated in restoration practice in South Africa, as well as challenges encountered in building this evidence base. Contrary to a recent assessment of this evidence base which found weaknesses, respondents viewed it as adequate and cited few obstacles to its development. Obstacles cited were mostly associated with planning and resource availability. We suggest that the disparity between practitioners’ perceptions and observed weaknesses in the evidence base could be a challenge in advancing evidence-based restoration. We explore opportunities to overcome this disparity as well as the obstacles listed by practitioners. These opportunities involve a shift from practitioners as users of scientific knowledge and evidence, to practitioners involved in the co-production of evidence needed to increase the effectiveness of restoration interventions.
Managing Nature–Business as Usual: Resource Extraction Companies and Their Representations of Natural Landscapes
Authors: Brown Mark
Published: November 2015

This article contributes to knowledge of how one category of business organization, very large, British-based, natural resource extraction corporations, has begun to manage its operations for sustainability. The object of study is a large volume of texts that make representations of the managing-for-sustainability practices of these multinational corporations (MNCs). The macro-level textual analysis identifies patterns in the wording of the representations of practice. Hajer’s understanding of discourse, in which ideas are contextualized within social processes of practice, provides the theoretical approach for discourse analysis that gives an insight into how they understand and practice sustainability. Through this large-scale discourse analysis, illustrated in the article with specific textual examples, one can see that these natural resource MNCs are developing a vocabulary and a “grammar” which enables them to manage natural spaces in the same way that they are able to manage their own far-flung business operations. They make simplified representations of the much more complex natural landscapes in which their operations are sited and these models of nature can then be incorporated into the corporations’ operational management processes. Their journey towards sustainability delivers, in practice, the management of nature as business continues as usual.
Natural Products as Tools for Defining How Cellular Metabolism Influences Cellular Immune and Inflammatory Function during Chronic Infection
Authors: Lovelace Erica , Polyak Stephen
Published: November 2015

Chronic viral infections like those caused by hepatitis C virus (HCV) and human immunodeficiency virus (HIV) cause disease that establishes an ongoing state of chronic inflammation. While there have been tremendous improvements towards curing HCV with directly acting antiviral agents (DAA) and keeping HIV viral loads below detection with antiretroviral therapy (ART), there is still a need to control inflammation in these diseases. Recent studies indicate that many natural products like curcumin, resveratrol and silymarin alter cellular metabolism and signal transduction pathways via enzymes such as adenosine monophosphate kinase (AMPK) and mechanistic target of rapamycin (mTOR), and these pathways directly influence cellular inflammatory status (such as NF-κB) and immune function. Natural products represent a vast toolkit to dissect and define how cellular metabolism controls cellular immune and inflammatory function.
A concise and efficient synthesis of benzimidazo[1,2-c]quinazolines through CuI-catalyzed intramolecular N-arylations
Authors: Pang Xinlong , Chen Chao , Li Ming , Xi Chanjuan
Published: November 2015

A series of functionalized benzimidazo[1,2-c]quinazoline derivatives was obtained in excellent yields under mild conditions through a CuI-catalyzed Ullmann N-arylation starting from easily available starting materials.
Low prevalences of HIV infection and HSV genital shedding in the general adult female population in Senegal
Authors: Diawara Silman , Bélec Laurent , Dem Ahmadou , Mbaye Seydou , Diop Ndiaye Halimatou , Matta Mathieu , Bousso Gueye Sokhna , Mboup Souleymane , Toure Kane Coumba
Published: November 2015

Introduction: Herpes simplex virus (HSV) is the main co-factor for heterosexual transmission of the human immunodeficiency virus (HIV) in sub-Saharan Africa, and could be involved in the dynamics of the HIV epidemic in Senegal. Methodology: Genital shedding of HSV was evaluated in adult females who had visited the provincial healthcare centres in Diass, Louga, and Kebemer in Senegal. Study subjects were interviewed by a healthcare worker for sociodemographic characteristics and sexual behavior, and HIV serology was offered. In addition, cervical secretion lavage samples were evaluated for HSV DNA by real-time polymerase chain reaction (PCR), the melting curve analysis of which permitted distinction between HSV type 1 (HSV-1) and HSV type 2 (HSV-2). Results: Among 302 women (mean age, 40 years) enrolled, none were infected by HIV. The mean age at first sexual intercourse was 20 years, and the mean number of sexual partners in the previous year was 1.3 (range, 1–7). Only 6 of 302 (1.9%) women had cervico-vaginal secretions positive for HSV DNA. No association between HSV DNA shedding and any sociodemographic or biological variables was found. Surprisingly, genital shedding of HSV-1 was found in two (0.7%) women, representing 33% of herpes-shedding women, and HSV-2 in four (1.5%) women. Conclusions: Taken together, our observations indicate a low prevalence of HSV DNA genital shedding in adult Senegalese women.
Seroprevalence and predictors of hepatitis E infection in Nigerian children
Authors: Ekanem Emmanuel , Ikobah Joanah , Okpara Henry , Udo Jacob
Published: November 2015

Introduction: Hepatitis E is a hepatotropic virus transmitted through the fecal-oral route and is prevalent in developing countries where sanitation is still a public health issue. There is no epidemiological data about this virus in Nigerian children. All the existing studies are hospital based, with obvious limitations. This study was conducted to establish the seroprevalence and predictors of viral hepatitis E antibody in children in Akpabuyo Local Government Area of Cross River State, Nigeria. Methodology: This was a community-based, cross-sectional study. A multi-staged sampling technique was used to select ten communities from which 406 children were recruited. The study period was April to June 2012. A structured interviewer-administered questionnaire was used for data collection. Blood samples were screened for anti-HEV IgG antibody using the enzyme-linked immunoassay technique. Multivariate logistic regression was used to identify factors that independently predicted the occurrence of the anti-HEV IgG antibody. A p value of < 0.05 was considered significant. Results: The seroprevalence rate of anti-HEV IgG antibody was 7.7% (95% CI = 5.1–10.3). The study population mainly (94.1%) comprised the lower social class. Levels of social amenities in these communities were generally poor, with virtually no piped water and modern sewage disposal systems. After multivariate analysis, the predictor of infection was the duration of residence in the study communities. Conclusions: HEV infection was prevalent in the study population. Educational campaigns and provision of good sewage disposal and piped water are of high necessity.
Relationship between vitamin D and human immunodeficiency virus (HIV) viral load among HIV-infected patients in Kazakhstan
Authors: Nugmanova Zhamilya , Patel Nimish , Akhmetova Gulzhakhan M , Kurmangalieva Gulnara S , Abdumananova Malika K , Akanov Aikan A , Kovtunenko Natalia G , McNutt Louise-Anne
Published: November 2015

Introduction: Human immunodeficiency virus (HIV) is associated with inflammation. An association between vitamin D deficiency and inflammation also exists. Our study attempts to examine whether there may be a relationship between vitamin D and HIV viral load (HIV RNA) by: 1) characterizing the distribution of 25-hydroxyvitamin D (25-OHD), and 2) determining if 25-OHD is independently associated with HIV RNA. Methodology: A cross-sectional study among HIV-infected adults was conducted. Demographics, clinical / social / HIV characteristics and data on antiretroviral therapy were collected by questionnaire, medical records and laboratory testing. All patients provided blood samples. Bivariate and multivariate analyses were conducted to quantify the relationship between vitamin D and HIV RNA. Results: Among the 564 patients, the median (interquartile range, IQR) 25-OHD value was 24.42 (16.22 – 34.10) ng/mL. The mean (standard deviation, SD) log-HIV RNA was 3.51 (1.11) copies/mL. There were 304 patients (53.9%) with an undetectable HIV RNA (< 500 copies/mL). In the bivariate analyses, no differences were observed between patients with and without an undetectable HIV RNA in mean (SD) 25-OHD, 25-OHD insufficiency (< 30 ng/mL), or 25-OHD deciles. In the log-binomial regression analyses, there was no association between 25-OHD and an undetectable HIV RNA (prevalence ratio: 1.00, 95% confidence interval: 0.99 – 1.01, p = 0.67). Conclusions: No relationship was observed between 25-OHD and HIV RNA among HIV-infected patients in Kazakhstan.
Effectiveness of a superoxide dismutase supplement derived from melon extract as a recovery aid for horses following strenuous exercise
Authors: Walker A.J. , Avenatti R.C. , Arent S.M. , McKeever K.H.
Published: November 2015

An antioxidant derived from a variety of melon purported to be high in superoxide dismutase was fed to horses and the effect on performance and recovery was measured following repeated daily bouts of intense exercise. In a cross-over design study, six unfit Standardbred mares were fed this antioxidant at a rate of 2.0 IU/kg body weight (BW)/d as part of the daily ration for 4 weeks before undergoing a simulated race test (SRT) repeated over a 3-day period. The SRT, designed to simulate the training of Standardbreds, consisted of a 2 min warm-up at 6 m/s followed by a high speed run to fatigue at 125% of the speed calculated to correspond to the horse’s maximal aerobic capacity (VO2max) as determined in a baseline incremental exercise test. Measurements included VO2 and the VO2 recovery time, VO2 at the point of recovery, run time, as well as plasma lactate, and plasma cortisol. Oxidative stress was measured by assessing malondialdehyde (MDA). The results from the study demonstrated that there were no differences (P>0.05) in mean values for the plasma concentrations of lactate, cortisol, or MDA. However, when supplemented with this antioxidant, there was an observable pattern with a small-to-medium effect size of an increase average run time of 14 s. Another important observation was that despite the longer run time, there was a moderate effect for a faster VO2 recovery time. The data suggest that supplementation with 2.0 IU/kg BW of this antioxidant had a modest but non-significant effect on performance and recovery time. Additionally, it did not negatively impact physiology in untrained Standardbred mares.
Energy intake and expenditure of improvised explosive device detection dogs
Authors: Phillips S. Pratt , Kutzner-Mulligan J. , Davis M.
Published: November 2015

Improvised explosive device detection (IDD) dogs explore up to 40 km of land daily and therefore have energetic demands that may be above the National Research Council’s requirement for working dogs. This study was designed to quantify metabolic energy intake (MEI) and total energy expenditure (TEE) in a group of IDD dogs. Two groups of dogs that had undergone different training protocols (CP1, n=8 and CP2, n=11) underwent a 5-day deployment simulation that consisted of combined road clearing, orbit and point-to-point activities and lasted approximately 9 h per day. The CP1 dogs were fed according to the IDD Marine Corps Manual, while CP2 dogs were offered additional calories based on pilot study data of energy expenditure. The MEI was calculated based on feed intake rates and chemical composition of the diets. TEE was quantified using the doubly-labelled water technique in 2 of the CP1 dogs and 7 of the CP2 dogs. During the 5-day deployment simulation the MEI ranged from 189-310 kcal/bodyweight (BW)0.75 per day, with the CP2 dogs at the higher end because they were offered more feed. The TEE ranged between 375-507 kcal/BW0.75 per day, above the MEI, suggesting the dogs were in negative energy balance and metabolic reserves within the body were combusted for energy production. These findings reveal that energy requirements of deployed military working dogs are higher than previously published metabolic energy requirements of working dogs.
Sequence homology of the fly proteins tropomyosin, arginine kinase and myosin light chain with known allergens in invertebrates
Authors: Romero M.R. , Claydon A.J. , Fitches E.C. , Wakefield M.E. , Charlton A.J.
Published: November 2015

Due to the rapid growth of the world’s population and the increasing demand for food, there is an urgent need for alternative, more sustainable sources of protein. Insects have an important role in the diet in some societies and current initiatives are exploring the potential that insects have to offer for the production of food and feed. In this context, the safety implications of both producing and consuming insects are an important aspect to investigate. Here we present a bioinformatics analysis of proteomics data obtained for larvae of four different species of fly to assess the homology of tropomyosin, arginine kinase and myosin light chain with the crustacean orthologous proteins and other known allergenic proteins. The results indicate that the three proteins share homology with known allergens and therefore it is likely that they are also potential allergens. The implications in relation to mass rearing of flies are discussed.
Fusión en hornos de cubilote para recarburación de hierro de reducción directa
Authors: Enríquez José L. , Tremps Enrique , Ruiz-Bustinza Iñigo , Morón Carlos , García-García Alfonso , Robla José I. , González-Gasca Carmen
Published: November 2015

Se describe la síntesis de aleaciones saturadas hierro-carbono (fundiciones) en hornos de cubilote a partir de hierro de reducción directa. Se revisan sus fundamentos, operaciones realizadas, resultados y conclusiones. Finalmente se ofrecen recomendaciones para su implantación industrial.
Total Variation Applications in Computer Vision
Authors: Estrela Vania Vieira , Magalhães Hermes Aguiar , Saotome Osamu
Published: November 2015

The objectives of this chapter are: (i) to introduce a concise overview of regularization; (ii) to define and to explain the role of a particular type of regularization called total variation norm (TV-norm) in computer vision tasks; (iii) to set up a brief discussion on the mathematical background of TV methods; and (iv) to establish a relationship between models and a few existing methods to solve problems cast as TV-norm. For the most part, image-processing algorithms blur the edges of the estimated images, however TV regularization preserves the edges with no prior information on the observed and the original images. The regularization scalar parameter ? controls the amount of regularization allowed and it is essential to obtain a high-quality regularized output. A wide-ranging review of several ways to put into practice TV regularization as well as its advantages and limitations are discussed.
Collective Event Detection by a Distributed Low-Cost Smart Camera Network
Authors: Hwang Jhih-Yuan , Lee Wei-Po
Published: November 2015

The current surveillance systems must identify the continuous human behaviors to detect various events from video streams. To enhance the performance of event recognition, in this chapter, we propose a distributed low-cost smart cameras system, together with a machine learning technique to detect abnormal events through analyzing the sequential behaviors of a group of people. Our system mainly includes a simple but efficient strategy to organize the behavior sequence, a new indirect encoding scheme to represent a group of people with relatively few features, and a multi-camera collaboration strategy to perform collective decision making for event recognition. Experiments have been conducted and the results confirm the reliability and stability of the proposed system in event recognition.
Cloud-Based Image Fusion Using Guided Filtering
Authors: Shukla Piyush Kumar , Dixit Madhuvan
Published: November 2015

Current image coding with image fusion schemes make it hard to utilize external images for transform even if highly correlated images can be found in the cloud. To solve this problem, we explain an approach of cloud-based image transform coding with image fusion methodwhich is distinguish from exists image fusion method. A fast and efficient image fusion technique is proposed for creating a highly generated fused image through merging multiple corresponding images. The proposed technique is based on a two-scale decomposition of an image into a low layer containing large scale variations, and a detail layer acquiring small scale details. A novel approach of guided filtering-based weighted average method is proposed to make full use of spatial consistency for merge of the base and detail layers. Analytical results represent that the proposed technique can obtain state-of-the-art performance for image fusion of multispectral, multifocus, multimodal, and multiexposure images.
Determination of Stability of Rock Slope Using Intelligent Pattern Recognition Techniques
Authors: Chowdhury Swaptik , Goyal Pratik , Hariharan R. , Samui Pijush
Published: November 2015

This article adopts Minimax Probability Machine (MPM) and Extreme Learning Machine (ELM) for prediction of stability status of rock slope. The proposed MPM and ELM models use unit weight (?), cohesions (cA) and (cB), angles of internal friction (?A) and ?B, angle of the line of intersection of the two joint-sets (?p), slope angle (?f), and height (H) as input parameters. For this chapter the determination of stability of rock slope has been adopted as classification problem. The developed MPM and ELM have been compared with each other. The results of this article shows that the developed MPM is robust model for prediction of stability status of rock slope.