bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-wide microRNA profiles identify miR-107 as a top miRNA associating with expression of the CYP3As and other drug metabolizing cytochrome P450 enzymes in the liver
Authors: Tantawy Marwa , Collins Joseph M. , Wang Danxin
Published: August 2022

Cytochrome P450 (CYP) drug metabolizing enzymes are responsible for the metabolism of over 70% of currently used medications with the CYP3A family being the most important CYP enzymes in the liver. Large inter-person variability in expression/activity of the CYP3As greatly affects drug exposure and treatment outcomes, yet the cause of such variability remains elusive. Micro-RNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression and are involved in diverse cellular processes including metabolism of xenobiotics and therapeutic outcomes. Target prediction and in vitro functional assays have linked several miRNAs to the control of CYP3A4 expression. Yet, their co-expression with CYP3As in the liver remain unclear. In this study, we used genome-wide miRNA profiling in liver samples to identify miRNAs associated with the expression of the CYP3As. We identified and validated both miR-107 and miR-1260 as strongly associated with the expression of CYP3A4, CYP3A5, and CYP3A43. Moreover, we found associations between miR-107 and nine transcription factors (TFs) that regulate CYP3A expression, with estrogen receptor alpha (ESR1) having the largest effect size. Including ESR1 and the other TFs in the regression model either diminished or abolished the associations between miR-107 and the CYP3As, indicating that the role of miR-107 in CYP3A expression may be indirect and occur through these key TFs. Indeed, testing the other nine CYPs previously shown to be regulated by ESR1 identified similar miR-107 associations that were dependent on the exclusion of ESR1 and other key TFs in the regression model. In addition, we found significant differences in miRNA expression profiles in liver samples between race and sex. Together, our results identify miR-107 as a potential epigenetic regulator that is strongly associated with the expression of many CYPs, likely via impacting the CYP regulatory network controlled by ESR1 and other key TFs. Therefore, both genetic and epigenetic factors that alter the expression of miR-107 may have a broad influence on drug metabolism.
Multimodal neuroimage data fusion based on multikernel learning in personalized medicine
Authors: Ran Xue , Shi Junyi , Chen Yalan , Jiang Kui
Published: August 2022

Neuroimaging has been widely used as a diagnostic technique for brain diseases. With the development of artificial intelligence, neuroimaging analysis using intelligent algorithms can capture more image feature patterns than artificial experience-based diagnosis. However, using only single neuroimaging techniques, e.g., magnetic resonance imaging, may omit some significant patterns that may have high relevance to the clinical target. Therefore, so far, combining different types of neuroimaging techniques that provide multimodal data for joint diagnosis has received extensive attention and research in the area of personalized medicine. In this study, based on the regularized label relaxation linear regression model, we propose a multikernel version for multimodal data fusion. The proposed method inherits the merits of the regularized label relaxation linear regression model and also has its own superiority. It can explore complementary patterns across different modal data and pay more attention to the modal data that have more significant patterns. In the experimental study, the proposed method is evaluated in the scenario of Alzheimer’s disease diagnosis. The promising performance indicates that the performance of multimodality fusion via multikernel learning is better than that of single modality. Moreover, the decreased square difference between training and testing performance indicates that overfitting is reduced and hence the generalization ability is improved.
The roles of glycolysis in osteosarcoma
Authors: Feng Zuxi , Ou Yanghuan , Hao Liang
Published: August 2022

Metabolic reprogramming is of great significance in the progression of various cancers and is critical for cancer progression, diagnosis, and treatment. Cellular metabolic pathways mainly include glycolysis, fat metabolism, glutamine decomposition, and oxidative phosphorylation. In cancer cells, reprogramming metabolic pathways is used to meet the massive energy requirement for tumorigenesis and development. Metabolisms are also altered in malignant osteosarcoma (OS) cells. Among reprogrammed metabolisms, alterations in aerobic glycolysis are key to the massive biosynthesis and energy demands of OS cells to sustain their growth and metastasis. Numerous studies have demonstrated that compared to normal cells, glycolysis in OS cells under aerobic conditions is substantially enhanced to promote malignant behaviors such as proliferation, invasion, metastasis, and drug resistance of OS. Glycolysis in OS is closely related to various oncogenes and tumor suppressor genes, and numerous signaling pathways have been reported to be involved in the regulation of glycolysis. In recent years, a vast number of inhibitors and natural products have been discovered to inhibit OS progression by targeting glycolysis-related proteins. These potential inhibitors and natural products may be ideal candidates for the treatment of osteosarcoma following hundreds of preclinical and clinical trials. In this article, we explore key pathways, glycolysis enzymes, non-coding RNAs, inhibitors, and natural products regulating aerobic glycolysis in OS cells to gain a deeper understanding of the relationship between glycolysis and the progression of OS and discover novel therapeutic approaches targeting glycolytic metabolism in OS.
Case report: Kinetics of human leukocyte antigen receptor HLA-DR during liver injury induced by potassium para-aminobenzoate as assessed for causality using the updated RUCAM
Authors: Plüß Marlene , Tampe Désirée , Schwörer Harald , Bremer Sebastian Christopher Benjamin , Tampe Björn
Published: August 2022

Potassium para-aminobenzoate (POTABA) is used to treat Peyronie’s disease by decreasing fibrosis and plaque size progression. Among potential side effects, drug-induced liver injury (DILI) attributed to POTABA administration has been reported in a few cases and inferred to immune hypersensitivity. In the present case, we investigated clinical,biochemical, and serological features as well as searched for non-drug-related causes, and applied the updated Roussel Uclaf Causality Assessment Method (RUCAM) confirming a highly probable causality of POTABA-induced liver injury. Moreover, we here observed specific activated CD3+T lymphocytes during the acute phase of liver injury by monitoring of human leukocyte antigen receptor (HLA-DR) expression. Furthermore, improvement of biochemical markers of liver injury after POTABA withdrawal was associated with a rapid decline of CD3+HLA-DR+immune cells. In contrast, CD14+monocytes expressing HLA-DR remained stable during recovery from liver injury. These observations implicate a specific involvement of activated T lymphocytes in liver injury mediated by POTABA. Clinicians should be aware of POTABA-induced liver injury, and measurement of activated immune cells by assessment of HLA-DR could provide pathomechanistic insights enabling biomonitoring of recovery from DILI.
Impact of extracorporeal membrane oxygenation on voriconazole plasma concentrations: A retrospective study
Authors: Ye Qinghua , Yu Xin , Chen Wenqian , Li Min , Gu Sichao , Huang Linna , Zhan Qingyuan , Wang Chen
Published: August 2022

Aims: We aimed to assess the impact of extracorporeal membrane oxygenation (ECMO) on voriconazole exposure.

Methods: Adult critically ill patients with or without ECMO support receiving intravenous voriconazole therapy were included in this retrospective study conducted in a tertiary referral intensive care unit. The first therapeutic drug monitoring (TDM) results of voriconazole in ECMO patients and non-ECMO patients were collected, and the prevalence of subtherapeutic concentrations was analyzed. Multivariate analyses were performed to evaluate the effect of ECMO on voriconazole exposure.

Results: A total of 132 patients (including 66 patients with ECMO support) were enrolled and their respective first voriconazole trough concentrations (Cmin) were recorded. The median Cmin of the ECMO group and the non-ECMO group was 1.9 (1.4–4.4) and 4.4 (3.2–6.9) mg/L, respectively (p = 0.000), and the proportion of the two groups in subtherapeutic concentrations range (<2 mg/L) was 51.5% and 7.6%, respectively (p = 0.000). Multiple linear regression analysis of voriconazole Cmin identified that the use of ECMO and coadministration of glucocorticoids were associated with significantly reduced concentrations, while increasing SOFA score and increasing daily dose were associated with significantly increased concentrations. The model accounted for 32.2% of the variability of voriconazole Cmin. Furthermore, binary logistic regression demonstrated that the use of ECMO was an independent risk factor (OR = 7.78, p = 0.012) for insufficient voriconazole exposure.

Conclusion: Our findings showed that, in addition to the known drug interactions, ECMO is a significant covariable affecting voriconazole exposure. In addition, SOFA score was identified as a factor associated with increased voriconazole concentration.
A vanadium dioxide-based metamaterial with quatrefoil and circle loaded structure on flexible polyamide substrate for terahertz applications
Authors: Abdulkarim Yadgar I. , Awl Halgurd N. , Muhammadsharif Fahmi F. , Bakır Mehmet , Alkurt Fatih Özkan , Altıntaş Olcay , Saeed Salah Raza , Taouzari Mohamed , Karaaslan Muharrem , Appasani Bhargav , Dong Jian
Published: August 2022

In this study, a novel broadband metamaterial (MTM) absorber is proposed, which is based on vanadium dioxide (VO2) resonators coated onto a flexible polyamide substrate. The VO2 resonator is in the form of a quatrefoil and circle-loaded complementary square split ring resonator (CSSRR), which is fine tuned to operate in the terahertz range. The MTM design and simulation were carried out using Computer Simulation Technology (CST) software, by which different layouts were investigated to achieve the optimum structure. The results showed the presence of two pronounced peaks for the proposed MTM structure at 0.88 and 1.42 THz. The absorption peaks were found to be insensitive to the wave polarization and incident angle from 0° to 90°. The absorption mechanism of the MTM was investigated considering the insulating and metallic states of VO2. The results were in a good agreement with those calculated using high frequency simulated structure (HFSS) software. The flexible nature of the proposed MTM design makes it a viable candidate for non-planar and conformal geometry applications in stealth technology, and terahertz imaging.
Design of a vacuum system for space active hydrogen maser
Authors: Que Haohui , Xu Wujiabei , Li Qi , Xiang Jie , Lu Tao , Liu Tiexin , Cai Yong , Dai Jiayu , Hou Xueling
Published: August 2022

With the high precision and stability of its frequency signal outputs, active hydrogen maser plays an important role in such fields as timing, satellite navigation, and communication. However, it needs to be lighter so as to be applied in space. We made a research, based on the calculation of the hydrogen flow and the adsorption efficiency of the adsorption unit, on the parameters of the vacuum system and the structural requirements, and designed a combined vacuum pump for the Space Active Hydrogen Maser (SAHM). This vacuum pump consists of a getter pump and a small ion pump, the total mass of which is about 5 kg. The pumping speed will be about 474 L/s by computation, when an amount, 2.5 MPa L, of hydrogen has been adsorbed by getters. Theoretically, the total source hydrogen inflow in lifetime is not higher than 20% of the total capacity getter pump, thus the design should amply meet the requirements of the SAHM vacuum system, and is of great significance for future SAHM applications.
PEDOT: PSS promotes neurogenic commitment of neural crest-derived stem cells
Authors: Pisciotta Alessandra , Lunghi Alice , Bertani Giulia , Di Tinco Rosanna , Bertoni Laura , Orlandi Giulia , Biscarini Fabio , Bianchi Michele , Carnevale Gianluca
Published: August 2022

Poly (3,4-ethylendioxythiophene) polystyrene sulphonate (PEDOT:PSS) is the workhorse of organic bioelectronics and is steadily gaining interest also in tissue engineering due to the opportunity to endow traditional biomaterials for scaffolds with conductive properties. Biomaterials capable of promoting neural stem cell differentiation by application of suitable electrical stimulation protocols are highly desirable in neural tissue engineering. In this study, we evaluated the adhesion, proliferation, maintenance of neural crest stemness markers and neurogenic commitment of neural crest-derived human dental pulp stem cells (hDPSCs) cultured on PEDOT:PSS nanostructured thin films deposited either by spin coating (SC-PEDOT) or by electropolymerization (ED-PEDOT). In addition, we evaluated the immunomodulatory properties of hDPSCs on PEDOT:PSS by investigating the expression and maintenance of the Fas ligand (FasL). We found that both SC-PEDOT and ED-PEDOT thin films supported hDPSCs adhesion and proliferation; however, the number of cells on the ED-PEDOT after 1 week of culture was significantly higher than that on SC-PEDOT. To be noted, both PEDOT:PSS films did not affect the stemness phenotype of hDPSCs, as indicated by the maintenance of the neural crest markers Nestin and SOX10. Interestingly, neurogenic induction was clearly promoted on ED-PEDOT, as indicated by the strong expression of MAP-2 and β—Tubulin-III as well as evident cytoskeletal reorganisation and appreciable morphology shift towards a neuronal-like shape. In addition, strong FasL expression was detected on both undifferentiated or undergoing neurogenic commitment hDPSCs, suggesting that ED-PEDOT supports the expression and maintenance of FasL under both expansion and differentiation conditions.
Mistimed sleep and waking activity in humans disrupts glucocorticoid signalling transcripts and SP1, but not plasma cortisol rhythms
Authors: Archer Simon N. , Möller-Levet Carla S. , Laing Emma E. , Dijk Derk-Jan
Published: August 2022

Cortisol is a robust circadian signal that synchronises peripheral circadian clocks with the central clock in the suprachiasmatic nucleusviaglucocorticoid receptors that regulate peripheral gene expression. Misalignment of the cortisol rhythm with the sleep–wake cycle, as occurs in shift work, is associated with negative health outcomes, but underlying molecular mechanisms remain largely unknown. We experimentally induced misalignment between the sleep–wake cycle and melatonin and cortisol rhythms in humans and measured time series blood transcriptomics while participants slept in-phase and out-of-phase with the central clock. The cortisol rhythm remained unchanged, but many glucocorticoid signalling transcripts were disrupted by mistimed sleep. To investigate which factors drive this dissociation between cortisol and its signalling pathways, we conducted bioinformatic and temporal coherence analyses. We found that glucocorticoid signalling transcripts affected by mistimed sleep were enriched for binding sites for the transcription factor SP1. Furthermore, changes in the timing of the rhythms ofSP1transcripts, a major regulator of transcription, and changes in the timing of rhythms in transcripts of the glucocorticoid signalling pathways were closely associated. Associations between the rhythmic changes in factors that affect SP1 expression and its activity, such as STAT3, EP300, HSP90AA1, and MAPK1, were also observed. We conclude that plasma cortisol rhythms incompletely reflect the impact of mistimed sleep on glucocorticoid signalling pathways and that sleep–wake driven changes in SP1 may mediate disruption of these pathways. These results aid understanding of mechanisms by which mistimed sleep affects health.
Identification and functional analysis of Tex11 and Meig1 in spermatogenesis of Hyriopsis cumingii
Authors: Huo Yingduo , Gu Yang , Cao Mulian , Mao Yingrui , Wang Yayu , Wang Xiaoqiang , Wang Guiling , Li Jiale
Published: August 2022

Abstract: The process of spermatogenesis is complex and controlled by many genes. In mammals, Testis-expressed gene 11 (Tex11) and meiosis expressed gene 1 (Meig1) are typical spermatogenesis-related genes. In this study, we obtained the full length cDNAs for Tex11 (3143bp) and Meig1 (1649bp) in Hyriopsis cumingii by cloning. Among them, Hc-Tex11 contains 930 amino acids and Hc-Meig1 contains 91 amino acids. The protein molecular masses (MW) of Hc-Tex11 and Hc-Meig1 were 105.63 kDa and 10.95 kDa, respectively. Protein secondary structure analysis showed that Hc-TEX11 protein has three TPR domains. The expression of Hc-Tex11 and Hc-Meig1 in different tissues showed higher levels in testes. At different ages, the expression of Hc-Tex11 and Hc-Meig1 was higher levels in 3-year-old male mussels. During spermatogenesis, the mRNA levels of Hc-Tex11, Hc-Meig1 gradually increased with the development of spermatogonia and reached a peak during sperm maturation. Hc-Tex11 and Hc-Meig1 mRNA signals were detected on spermatogonia and spermatocytes by in situ hybridization. In addition, RNA interference (RNAi) experiments of Hc-Tex11 caused a down-regulated of Dmrt1, KinaseX, Tra-2 and Klhl10 genes and an up-regulated of β-catenin gene. Based on the above experimental results, it can be speculated that Hc-Tex11 and Hc-Meig1 are important in the development of the male gonadal and spermatogenesis in H. cumingii, which can provide important clues to better comprehend the molecular mechanism of Tex11 and Meig1 in regulating spermatogenesis of bivalves.
Physical active lifestyle promotes static and dynamic balance performance in young and older adults
Authors: Sarto Fabio , Pizzichemi Martina , Chiossi Francesco , Bisiacchi Patrizia S. , Franchi Martino V , Narici Marco V , Monti Elena , Paoli Antonio , Marcolin Giuseppe
Published: August 2022

Although regular physical activity exposure leads to positive postural balance control (PBC) adaptations, few studies investigated its effects, or the one of inactivity, on PBC in populations of different age groups. Thus, this study investigated the impact of a physically active lifestyle on static and dynamic PBC in young and older adults. Thirty-five young physically active subjects (YA), 20 young sedentary subjects (YS), 16 physically active older adults (OA), and 15 sedentary older adults (OS) underwent a static and a dynamic PBC assessment. A force platform and an instrumented proprioceptive board were employed to measure the center of pressure (COP) trajectory and the anteroposterior oscillations, respectively. In static conditions, no significant differences were detected among groups considering the overall postural balance performance represented by the area of confidence ellipse values. Conversely, the YA highlighted a higher efficiency (i.e., lower sway path mean velocity) in PBC maintenance compared to the other groups (YA vs OA: p = 0.0057, Cohen’s d = 0.94; YA vs OS p = 0.043, d = 1.07; YA vs YS p = 0.08, d = 0.67). OS exhibited an overall worse performance in dynamic conditions than YA and YS. Surprisingly, no differences were found between YS and OA for all the static and dynamic parameters considered. In conclusion, our results suggest that a physically active lifestyle may promote static and dynamic balance performance in young and older adults, thus with potentially positive effects on the age-related decline of postural balance performance. Dynamic PBC assessment seems more sensitive in detecting differences between groups than the static evaluation.
Association of jasmonic acid priming with multiple defense mechanisms in wheat plants under high salt stress
Authors: Sheteiwy Mohamed S. , Ulhassan Zaid , Qi Weicong , Lu Haiying , AbdElgawad Hamada , Minkina Tatiana , Sushkova Svetlana , Rajput Vishnu D. , El-Keblawy Ali , Jośko Izabela , Sulieman Saad , El-Esawi Mohamed A. , El-Tarabily Khaled A. , AbuQamar Synan F. , Yang Haishui , Dawood Mona
Published: August 2022

Salinity is a global conundrum that negatively affects various biometrics of agricultural crops. Jasmonic acid (JA) is a phytohormone that reinforces multilayered defense strategies against abiotic stress, including salinity. This study investigated the effect of JA (60 μM) on two wheat cultivars, namely ZM9 and YM25, exposed to NaCl (14.50 dSm−1) during two consecutive growing seasons. Morphologically, plants primed with JA enhanced the vegetative growth and yield components. The improvement of growth by JA priming is associated with increased photosynthetic pigments, stomatal conductance, intercellular CO2, maximal photosystem II efficiency, and transpiration rate of the stressed plants. Furthermore, wheat cultivars primed with JA showed a reduction in the swelling of the chloroplast, recovery of the disintegrated thylakoids grana, and increased plastoglobuli numbers compared to saline-treated plants. JA prevented dehydration of leaves by increasing relative water content and water use efficiency via reducing water and osmotic potential using proline as an osmoticum. There was a reduction in sodium (Na+) and increased potassium (K+) contents, indicating a significant role of JA priming in ionic homeostasis, which was associated with induction of the transporters, viz., SOS1, NHX2, and HVP1. Exogenously applied JA mitigated the inhibitory effect of salt stress in plants by increasing the endogenous levels of cytokinins and indole acetic acid, and reducing the abscisic acid (ABA) contents. In addition, the oxidative stress caused by increasing hydrogen peroxide in salt-stressed plants was restrained by JA, which was associated with increased α-tocopherol, phenolics, and flavonoids levels and triggered the activities of superoxide dismutase and ascorbate peroxidase activity. This increase in phenolics and flavonoids could be explained by the induction of phenylalanine ammonia-lyase activity. The results suggest that JA plays a key role at the morphological, biochemical, and genetic levels of stressed and non-stressed wheat plants which is reflected in yield attributes. Hierarchical cluster analysis and principal component analyses showed that salt sensitivity was associated with the increments of Na+, hydrogen peroxide, and ABA contents. The regulatory role of JA under salinity stress was interlinked with increased JA level which consequentially improved ion transporting, osmoregulation, and antioxidant defense.
RNA structure mediated thermoregulation: What can we learn from plants?
Authors: Thomas Sherine E. , Balcerowicz Martin , Chung Betty Y.-W.
Published: August 2022

RNA molecules have the capacity to form a multitude of distinct secondary and tertiary structures, but only the most energetically favorable conformations are adopted at any given time. Formation of such structures strongly depends on the environment and consequently, these structures are highly dynamic and may refold as their surroundings change. Temperature is one of the most direct physical parameters that influence RNA structure dynamics, and in turn, thermosensitive RNA structures can be harnessed by a cell to perceive and respond to its temperature environment. Indeed, many thermosensitive RNA structures with biological function have been identified in prokaryotic organisms, but for a long time such structures remained elusive in eukaryotes. Recent discoveries, however, reveal that thermosensitive RNA structures are also found in plants, where they affect RNA stability, pre-mRNA splicing and translation efficiency in a temperature-dependent manner. In this minireview, we provide a short overview of thermosensitive RNA structures in prokaryotes and eukaryotes, highlight recent advances made in identifying such structures in plants and discuss their similarities and differences to established prokaryotic RNA thermosensors.
Identification of receptor-like proteins induced by Sclerotinia sclerotiorum in Brassica napus
Authors: Li Wei , Lu Junxing , Yang Chenghuizi , Xia Shitou
Published: August 2022

Heightening the resistance of plants to microbial infection is a widely concerned issue, especially for economical crops. Receptor-like proteins (RLPs), typically with tandem leucine-rich repeats (LRRs) domain, play a crucial role in mediating immune activation, being an indispensable constituent in the first layer of defense. Based on an analysis of orthologs among Brassica rapa, Brassica oleracea, and Brassica napus using Arabidopsis thaliana RLPs as a reference framework, we found that compared to A. thaliana, there were some obvious evolutionary diversities of RLPs among the three Brassicaceae species. BnRLP encoding genes were unevenly distributed on chromosomes, mainly on chrA01, chrA04, chrC03, chrC04, and chrC06. The orthologs of five AtRLPs (AtRLP3, AtRLP10, AtRLP17, AtRLP44, and AtRLP51) were highly conserved, but retrenchment and functional centralization occurred in Brassicaceae RLPs during evolution. The RLP proteins were clustered into 13 subgroups. Ten BnRLPs presented expression specificity between R and S when elicited by Sclerotinia sclerotiorum, which might be fabulous candidates for S. sclerotiorum resistance research.
Increasing the activities of protective enzymes is an important strategy to improve resistance in cucumber to powdery mildew disease and melon aphid under different infection/infestation patterns
Authors: Zhang Quancheng , Zhou Menghan , Wang Jungang
Published: August 2022

Powdery mildew, caused by Sphaerotheca fuliginea (Schlecht.) Poll., and melon aphids (Aphis gossypii Glover) are a typical disease and insect pest, respectively, that affect cucumber production. Powdery mildew and melon aphid often occur together in greenhouse production, resulting in a reduction in cucumber yield. At present there are no reports on the physiological and biochemical effects of the combined disease and pest infection/infestation on cucumber. This study explored how cucumbers can regulate photosynthesis, protective enzyme activity, and basic metabolism to resist the fungal disease and aphids. After powdery mildew infection, the chlorophyll and free proline contents in cucumber leaves decreased, while the activities of POD (peroxidase) and SOD (superoxide dismutase) and the soluble protein and MDA (malondialdehyde) contents increased. Cucumber plants resist aphid attack by increasing the rates of photosynthesis and basal metabolism, and also by increasing the activities of protective enzymes. The combination of powdery mildew infection and aphid infestation reduced photosynthesis and basal metabolism in cucumber plants, although the activities of several protective enzymes increased. Aphid attack after powdery mildew infection or powdery mildew infection after aphid attack had the opposite effect on photosynthesis, protective enzyme activity, and basal metabolism regulation. Azoxystrobin and imidacloprid increased the contents of chlorophyll, free proline, and soluble protein, increased SOD activity, and decreased the MDA content in cucumber leaves. However, these compounds had the opposite effect on the soluble sugar content and POD and CAT (catalase) activities. The mixed ratio of the two single agents could improve the resistance of cucumber to the combined infection of powdery mildew and aphids. These results show that cucumber can enhance its pest/pathogen resistance by changing physiological metabolism when exposed to a complex infection system of pathogenic microorganisms and insect pests.
Effect of cognitively engaging physical activity on executive functions in children
Authors: Bulten Rheanna , Bedard Chloe , Graham Jeffrey D. , Cairney John
Published: August 2022

Purpose

Physical activity (PA) has been shown to enhance executive functions (EFs) in children, and PA involving a cognitive component may confer additional benefit. The purpose of this study was to investigate whether cognitively engaging PA impacts EF, and whether affect and fitness act as either mediators or moderators of this relationship.

Methods

A randomized controlled trial was utilized to assess changes in EFs between a 20-min Dual Task (intervention condition), a PA Task (control condition), and a Cognitive Task (control condition). Children were scheduled for two visits in the INfant and Child Health (INCH) Lab at the University of Toronto. Physical fitness was assessed using the 20 m shuttle run, standing long jump, and grip strength tests. EFs were assessed using the Stroop Task, Trail Making Task (TMT), and Forward Working Memory Task (FWMT).

Results

38 children (Mage = 11.95 years, SD = 0.49, 61% female) participated. Repeated measures ANOVA showed main interactions between time on inhibition scores (p < 0.05, ηp2 = 0.489), and positive affect scores (p < 0.01, ηp2 = 0.284). Interaction effects between condition and time were not significant (p = 0.787, ηp2 = 0.014, p = .333, ηp2 = 0.061, p = 0.799, ηp2 = 0.013 for inhibition, switching, and passive working memory, respectively). Results showed no significant mediation effect of affect (95% CI = –0.5915, 2.147), or moderating effect between fitness and EF changes.

Conclusion

Possible explanations for these findings include inadequate cognitive engagement, lack of EF transfer, and statistical power. Results suggest any of these interventions may be beneficial for improving inhibition and positive affect in children.
A Russian Adaptation of the Emotional Contagion Scale
Authors: Kosonogov Vladimir , Kuskova Olga
Published: August 2022

The aim of the work was to develop and test the Russian version of the Emotional Contagion Scale. A sample of 518 volunteers from the general population filled in this questionnaire. We examined the one-factor model (all the items), the two-factor model (positive/negative), and the five-factor model (love/happiness/fear/anger/sadness). To measure its construct validity, we asked different subsamples to complete questionnaires of empathy and sensation seeking. The coefficients of test–retest reliability, internal consistency, and validity were acceptable. Only the one-factor model showed acceptable properties by all psychometric criteria. We also observed the gender effect, that is women were more contagious, according to the total scale and all subscales.
The inhibitory effect of moral emotions on malevolent creativity: Exploring the mediation role of emotional valence and prosocial behavior
Authors: Fu Hongyu , Zhang Zhonglu
Published: August 2022

The current study aims to investigate the influence of positive and negative moral emotions (gratitude and guilt) on malevolent creativity by exploring the potential mediation role of valence and prosocial behavior. Using autobiographical recall, three groups of participants developed gratitude, guilt, or neutral emotion, respectively, and then their prosocial behavior and malevolent creativity performance were compared. Results showed that compared with the neutral condition, individuals in the gratitude state experienced more positive emotions with less malevolent creative ideas, but the positive valence pathway had a positive effect on malevolent creativity, indicating the promoting effect of positive emotion on creativity. By contrast, individuals in the guilt state experienced more negative emotions, which result in less malevolent creativity. Gratitude and guilt promoted prosocial behaviors, which did not mediate the effect of gratitude or guilt on malevolent creativity. In short, the results indicate that the positive and negative moral emotions (gratitude and guilt) inhibit malevolent creativity, which is mediated by valence, instead of prosocial behavior.
Feasibility and acceptability of experience sampling among LGBTQ+ young people with self-harmful thoughts and behaviours
Authors: Williams A. J. , Arcelus Jon , Townsend Ellen , Michail Maria
Published: August 2022

This study was the first to determine whether it was feasible and acceptable to use experience sampling methods (ESM) among LGBTQ+ young people, who had current experiences of self-harm. Sixteen LGBTQ+ young people (16–25 years old) took part in the experience sampling study. This included a baseline assessment, a 7-day ESM assessment (participants were sampled six times a day using a phone app), and the option of an interview at the end of the 7-day ESM assessment. Feasibility data was descriptively analysed, with pilot ESM data presented. Qualitative data was thematically analysed to determine the acceptability (barriers and facilitators) of taking part in this study. Study feasibility was assessed by enrolment rate (55.2%), participant retention across assessment period (100%), ESM app feasibility (87.5%), and good adherence to total number of ESM surveys (67.6%). Individual study adherence ranged between 43 and 95.2%. Study acceptability was assessed by participant interviews. Thematic analysis indicated four superordinate themes; (i) Self-reflection and awareness; (ii) Practicalities of ESM surveys; (iii) Daily timeframes; and (iv) Suggestions for future studies. Pilot ESM data demonstrates that there was fluctuation of depressive and anxiety symptoms within- and between- participants over the course of the study, however, greater sample power is needed for full analysis. This study demonstrated that ESM designs are feasible and acceptable among LGBTQ+ young people with current experiences of self-harm. Pilot data indicated that specific experiences and moods are likely to be important to self-harm. These potentially have a temporal influence on self-harm behaviour or ideation, and therefore should be examined in a fully powered sample.
Research on the influence mechanism and governance mechanism of digital divide for the elderly on wisdom healthcare: The role of artificial intelligence and big data
Authors: Zhou Jian , Wang Zeyu , Liu Yang , Yang Jian
Published: August 2022

With the rapid development of digital information technology, life has become more convenient for people; however, the digital divide for the elderly was even more serious, so they became a forgotten group in the internet age over time. Residents' demand for healthcare is rising, but the wisdom healthcare service supported by digital information technology is less acceptable to the elderly due to the digital divide. Based on the knowledge gap theory and combining the value perception and satisfaction model, this study explores the influence of the digital divide for the elderly on wisdom healthcare satisfaction and takes the perceived value of wisdom healthcare as a mediator, and artificial intelligence and big data as moderators into the research framework. Based on the data of 1,052 elderly people in China, the results show that the digital divide for the elderly has a negative influence on wisdom healthcare satisfaction and perceived value. Moreover, it is found that wisdom healthcare perception value mediated the relationship between the digital divide for the elderly and the wisdom healthcare satisfaction, which enhances the negative effect of the digital divide for the elderly on wisdom healthcare satisfaction. Furthermore, the moderating effect of artificial intelligence and big data on the relationship between the digital divide for the elderly and the perceived value of wisdom healthcare is opposite to that between the perceived value of wisdom healthcare and wisdom healthcare satisfaction. Therefore, this study has a reference value for the development and optimization of smart medical industry.
Exploring the social value of Public Health Institutes: An international scoping survey and expert interviews
Authors: Ashton Kathryn , Green Liz , Clemens Timo , Parry-Williams Lee , Dyakova Mariana , Bellis Mark A.
Published: August 2022

Introduction

Making the case for investing in preventative public health by illustrating not only the health impact but the social, economic and environmental value of Public Health Institutes is imperative. This is captured by the concept of Social Value, which when measured, demonstrates the combined intersectoral value of public health. There is currently insufficient research and evidence to show the social value of Public Health Institutes and their work across the life course, population groups and settings, in order to make the case for more investment.

Methods

During July 2021, a quantitative online self-administered questionnaire was conducted across international networks. Semi-structured interviews were also carried out with nine representatives to gain a deeper understanding. A thematic analysis was undertaken on the data collected.

Results

In total, 82.3% (n = 14) were aware of the terminology of social value and 58.8% (n = 10) were aware of the economic method of Social Return on Investment. However, only two Institutes reported capturing social and community impacts within their economic analysis and only 41.2% (n = 7) currently capture or measure the social value of their actions. Interviews and survey responses indicate a lack of resources, skills and buy-in from political powers. Finally, 76.5% (n = 12) wanted to do more to understand and measure wider outcomes and impact of their actions. It was noted this can be achieved through enhancing political will, developing a community of best practice and tools.

Conclusion

This research can inform future work to understand how to measure the holistic social value of Public Health Institutes, in order to strengthen institutional capacity and impact, as well as to achieve a more equitable society, and a more sustainable health system and economy, making the case for investing in public health, as we recover from COVID-19.
Cultivation of Positive Psychological Quality of College Students' English Learning Under the Online and Offline Teaching Mode During the Epidemic
Authors: Yu Han , Li Xinguo
Published: August 2022

During the COVID-19 pandemic, long-term isolation and loneliness will cause college students' psychological fluctuations. Especially in online teaching, the lack of communication for a long time has led to a greatly reduced learning enthusiasm of college students. Therefore, this paper aims to explore the cultivation methods of the positive psychological quality of college students under the epidemic situation through the research on the positive psychology of college students' English learning. Aiming at the psychological status of college students, this paper focuses on analyzing the relationship between social support, psychological capital, and psychological health to explore more targeted ways of cultivating positive psychology. Because of the online and offline teaching mode, this paper focuses on analyzing the support environment of the online teaching mode, and analyses the current forms of English teaching. Experiments show that the direct path from psychological capital to mental health is not significant. However, the mediating path of psychological capital to mental health through social support was significant (p < 0.001). It shows that social support plays a complete mediating role, and the effect size of the mediation model reaches 49.70%. It shows that the current college students' English learning positive psychological quality is not high. In response to this, it is necessary to strengthen the tendency and ability to use social support and use the family environment to communicate more to achieve the cultivation of positive psychological quality.
Association between polyunsaturated fatty acid intake and infertility among American women aged 20–44 years
Authors: Wang Ruohan , Feng Ying , Chen Jiahe , Chen Yingjiao , Ma Fang
Published: August 2022

Background

Infertility is a nationwide public health priority in the U.S. However, few studies have investigated the effects of dietary intake of polyunsaturated fatty acids (PUFAs) on female infertility. This study explored the association between PUFA intake and risk of infertility.

Methods

A total of 1,785 women aged 20–44 years from three National Health and Nutrition Examination Survey cycles (2013–2018) were included in this cross-sectional study. The intake of PUFAs was obtained from a 24-h dietary interview on two separate days with a 3–10-day interval, and nutrient residue models were used. Fertility status was assessed by positive response to two relative questions via a questionnaire. Logistic regression models were used and some covariates were adjusted.

Results

Among all the participants, 340 (19.05%) women suffered from infertility. The intake of docosahexaenoic acid (DHA) (OR = 0.998, 95% CI 0.998, 0.009) was slightly related to the risk of infertility. In contrast, women with higher α-linolenic acid (ALA) (OR = 1.416, 95% CI 1.138, 1.763) and linoleic acid (LA) intake (OR = 1.020, 95% CI 1.002, 1.038) presented with a relatively higher risk of primary infertility. Furthermore, in 20–34-year-old women, higher omega-6/omega-3 was significant associated with the risk of infertility (OR = 1.002, 95%CI 1.000, 1.005).

Conclusions

Our results suggest that PUFA intake is only slightly associated with infertility. The higher the DHA intake, the lower the risk of infertility regardless of age. In women with primary infertility, ALA and LA has negative effect.
Drug sensitivity of clinical isolates of Mycobacterium tuberculosis and its association with bacterial genotype in the Somali region, Eastern Ethiopia
Authors: Worku Getnet , Gumi Balako , Girma Musse , Mohammedbirhan Binyam , Diriba Getu , Seid Getachew , Getu Melak , Amare Misikir , Sinshaw Waganeh , Ashagre Wondimu , Tschopp Rea , Carruth Lauren , Ameni Gobena
Published: August 2022

Background

Drug resistance is becoming a major bottleneck for tuberculosis (TB) control programs in countries with high TB burdens. Although several studies were conducted on the drug sensitivity of Mycobacterium tuberculosis (M. tuberculosis) in central Ethiopia, there is a lack of data on the drug sensitivity of M. tuberculosis in the peripheral regions of the country including in the Somali region. Therefore, the objective of this study was to evaluate the drug sensitivity of M. tuberculosis and its association with bacterial genotype and evaluate the performance of Xpert MTB/RIF (Xpert) in detecting resistance to rifampicin (RIF).

Methods

A total of 302 M. tuberculosis were tested using the BD BACTEC-Mycobacteria Growth Indicator Tube 960 (MGIT 960) system for their drug sensitivity to the first-line anti-TB drugs. Besides, the drug sensitivity of 10 multidrug-resistant (MDR) M. tuberculosis isolates was evaluated for the second-line anti-TB drugs. Additionally, 177 of the 302 isolates were tested for genotypic drug resistance using Xpert. Chi-square and Fisher's exact tests were used for the evaluation of the association between variables and drug sensitivity.

Results

The overall prevalence of resistance to at least one drug was 11.6% (95% CI: 7.9–15.2%), while the prevalence of MDR was 3.3% (95% CI: 1.3–5.3%). Two of the 10 MDR isolates were resistant to capreomycin. The spoligotype Shared International Type (SIT) 149 was significantly associated with either monoresistance or MDR (p < 0.05). Of the 177 isolates tested by Xpert, 6.2% (11/177) were RIF-resistant. Discordant between Xpert and MGIT 960 was observed in one isolate and linked with probe-binding delay (ΔCT max = 5.8). The sensitivity and specificity of the Xpert assay were 100 and 99.4%, respectively, while its positive and negative predictive values were 90.9 and 100%, respectively.

Conclusion

The magnitude of MDR M. tuberculosis in the Somali region of Ethiopia was higher than the national prevalence of MDR-TB warranting the strengthening of the TB control program in the Somali region. Besides, drug resistance was associated with SIT 149 spoligotype (genotype). The Xpert assay was observed to have high sensitivity and specificity in detecting RIF-resistant M. tuberculosis, which is encouraging for its application widely.
The impact of COVID-19 on self-reported burnout and health and mental health services in Nampula, Mozambique
Authors: Feliciano Paulino , Mootz Jennifer J. , Suleman Antonio , Su Austin Y. , Khan Saida , Gouveia Lidia , Santos Palmira , Wainberg Milton L. , Sweetland Annika C.
Published: August 2022

Background

The purpose of this study was to examine the impact of the COVID-19 pandemic on self-reported burnout of health workers, quality of care, and perceptions of COVID-19-related stigma in Mozambique.

Method

We conducted a cross-sectional quantitative assessment of 170 frontline health workers in Nampula District, Mozambique, including 149 (87.6%) primary care providers and 21 (12.4%) mental health specialists.

Results

Of the 170 frontline workers, only 10.1% of frontline workers were experiencing more professional burnout, whereas 33.3% felt it had lessened. The perceived impact on quality of care also varied, without any significant differences by sex, education level, or mental health training background. Compared to the beginning of the COVID-19 pandemic in March 2020, 42.3 and 38.1% providers felt that their ability to provide mental health and general health care, respectively, had worsened, compared to 57.7 and 61.9% who believed that either there was no change, or that the work had become easier. Likewise, 26.8% of providers felt that their ability to meet patients' needs had gotten more difficult, whereas 43.4% reported no change and 29.8% reported that it was easier. Twenty-two percent of providers reported an overall increase in caseloads since before the pandemic whereas the majority (67.9%) reported a decrease. Providers believed that 57.1% of people in general were afraid of people with COVID-19, 27.5% were afraid of a person recovered from COVID-19, and 39.9% were afraid of a person with family members with COVID-19. The perceived stigma about healthcare professionals was also low; only 27.4% believed that people in general were afraid of healthcare providers who deliver care to people with COVID-19.

Conclusion

In contrast with other global studies, many healthcare workers in our sample reported a reduction in burnout, which may be associated with the lower overall caseloads seen during this period. Similarly, the quality of care was minimally impacted. More research is needed to determine whether the experience in Mozambique is unique, or similarly observed in other low-resource settings.