bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Table 8 from Somatic Mosaic Chromosomal Alterations and Death of Cardiovascular Disease Causes among Cancer Survivors
Authors: Sun Maxine , Cyr Marie-Christyne , Sandoval Johanna , Lemieux Perreault Louis-Philippe , Busque Lambert , Tardif Jean-Claude , Dubé Marie-Pierre
Published: April 2023

Supplementary Table 8: The effect of mosaic loss of X chromosome on death of cardiovascular disease causes, coronary artery disease causes, from cancer, and any cause of death
Supplementary Table 7 from Somatic Mosaic Chromosomal Alterations and Death of Cardiovascular Disease Causes among Cancer Survivors
Authors: Sun Maxine , Cyr Marie-Christyne , Sandoval Johanna , Lemieux Perreault Louis-Philippe , Busque Lambert , Tardif Jean-Claude , Dubé Marie-Pierre
Published: April 2023

Supplementary Table 7: The effect of autosomal mosaic chromosomal alterations on death of cardiovascular disease causes, coronary artery disease causes, from cancer and any cause of death
Supplementary Table 16 from Somatic Mosaic Chromosomal Alterations and Death of Cardiovascular Disease Causes among Cancer Survivors
Authors: Sun Maxine , Cyr Marie-Christyne , Sandoval Johanna , Lemieux Perreault Louis-Philippe , Busque Lambert , Tardif Jean-Claude , Dubé Marie-Pierre
Published: April 2023

Supplementary Table 16: Cox regression analyses for the effect of mosaic chromosomal alterations on the risk of incident cardiovascular endpoints aged ≥65 years old (n=15,273)
Supplementary Table 10 from Somatic Mosaic Chromosomal Alterations and Death of Cardiovascular Disease Causes among Cancer Survivors
Authors: Sun Maxine , Cyr Marie-Christyne , Sandoval Johanna , Lemieux Perreault Louis-Philippe , Busque Lambert , Tardif Jean-Claude , Dubé Marie-Pierre
Published: April 2023

Supplementary Table 10: The effect of expanded mosaic chromosomal alterations on death of cardiovascular disease causes, coronary artery disease causes, from cancer, and any cause of death
Supplementary Figure 2 from Somatic Mosaic Chromosomal Alterations and Death of Cardiovascular Disease Causes among Cancer Survivors
Authors: Sun Maxine , Cyr Marie-Christyne , Sandoval Johanna , Lemieux Perreault Louis-Philippe , Busque Lambert , Tardif Jean-Claude , Dubé Marie-Pierre
Published: April 2023

Supplementary Figure 2: Visual mapping of mosaic chromosomal alterations
Supplementary Figure 1 from Somatic Mosaic Chromosomal Alterations and Death of Cardiovascular Disease Causes among Cancer Survivors
Authors: Sun Maxine , Cyr Marie-Christyne , Sandoval Johanna , Lemieux Perreault Louis-Philippe , Busque Lambert , Tardif Jean-Claude , Dubé Marie-Pierre
Published: April 2023

Supplementary Figure 1: Visual depiction of the proportion of patients with at least 1 mosaic chromosomal alteration
Table S9 from Exploring the Complex Relationship between Gut Microbiota and Risk of Colorectal Neoplasia Using Bidirectional Mendelian Randomization Analysis
Authors: Li Wanxin , Zhou Xuan , Yuan Shuai , Wang Lijuan , Yu Lili , Sun Jing , Chen Jie , Xiao Qian , Wan Zhongxiao , Zheng Ju-Sheng , Zhang Cai-Xia , Larsson Susanna C. , Farrington Susan M. , Law Philip , Houlston Richard S. , Tomlinson Ian , Ding Ke-Feng , Dunlop Malcolm G. , Theodoratou Evropi , Li Xue
Published: April 2023

Table S9 shows the reverse MR analyses of colorectal cancer, adenoma and polyps with the abundance of nine gut microbiota.
Table S8 from Exploring the Complex Relationship between Gut Microbiota and Risk of Colorectal Neoplasia Using Bidirectional Mendelian Randomization Analysis
Authors: Li Wanxin , Zhou Xuan , Yuan Shuai , Wang Lijuan , Yu Lili , Sun Jing , Chen Jie , Xiao Qian , Wan Zhongxiao , Zheng Ju-Sheng , Zhang Cai-Xia , Larsson Susanna C. , Farrington Susan M. , Law Philip , Houlston Richard S. , Tomlinson Ian , Ding Ke-Feng , Dunlop Malcolm G. , Theodoratou Evropi , Li Xue
Published: April 2023

Table S8 shows the forward MR analyses of the six gut microbial metabolites with the risk of colorectal cancer.
Table S7 from Exploring the Complex Relationship between Gut Microbiota and Risk of Colorectal Neoplasia Using Bidirectional Mendelian Randomization Analysis
Authors: Li Wanxin , Zhou Xuan , Yuan Shuai , Wang Lijuan , Yu Lili , Sun Jing , Chen Jie , Xiao Qian , Wan Zhongxiao , Zheng Ju-Sheng , Zhang Cai-Xia , Larsson Susanna C. , Farrington Susan M. , Law Philip , Houlston Richard S. , Tomlinson Ian , Ding Ke-Feng , Dunlop Malcolm G. , Theodoratou Evropi , Li Xue
Published: April 2023

Table S7 shows the forward MR analyses of nine gut microbiota with the risk of colorectal cancer.
Table S2 from Exploring the Complex Relationship between Gut Microbiota and Risk of Colorectal Neoplasia Using Bidirectional Mendelian Randomization Analysis
Authors: Li Wanxin , Zhou Xuan , Yuan Shuai , Wang Lijuan , Yu Lili , Sun Jing , Chen Jie , Xiao Qian , Wan Zhongxiao , Zheng Ju-Sheng , Zhang Cai-Xia , Larsson Susanna C. , Farrington Susan M. , Law Philip , Houlston Richard S. , Tomlinson Ian , Ding Ke-Feng , Dunlop Malcolm G. , Theodoratou Evropi , Li Xue
Published: April 2023

Table S2 shows the SNPs that associated with nine microbiota taxa (P<1×10-5).
Table S1 from Exploring the Complex Relationship between Gut Microbiota and Risk of Colorectal Neoplasia Using Bidirectional Mendelian Randomization Analysis
Authors: Li Wanxin , Zhou Xuan , Yuan Shuai , Wang Lijuan , Yu Lili , Sun Jing , Chen Jie , Xiao Qian , Wan Zhongxiao , Zheng Ju-Sheng , Zhang Cai-Xia , Larsson Susanna C. , Farrington Susan M. , Law Philip , Houlston Richard S. , Tomlinson Ian , Ding Ke-Feng , Dunlop Malcolm G. , Theodoratou Evropi , Li Xue
Published: April 2023

Table S1 shows the SNPs that associated with 24 microbiota taxa in MiBioGen (P<5×10-8).
Data from Somatic mosaic chromosomal alterations and death of cardiovascular disease causes among cancer survivors
Authors: Sun Maxine , Cyr Marie-Christyne , Sandoval Johanna , Lemieux Perreault Louis-Philippe , Busque Lambert , Tardif Jean-Claude , Dubé Marie-Pierre
Published: April 2023

Abstract
Background: Cancer survivors are at an increased risk of cardiovascular disease (CVD) compared to the general population. We sought to evaluate the impact of mosaic chromosomal alterations (mCAs) on death of CVD causes, coronary artery disease (CAD) causes, and of any cause in patients with a cancer diagnosis.
Methods: The study was a prospective cohort analysis of 48,919 UK Biobank participants with a cancer diagnosis. mCAs were characterized using DNA genotyping array intensity data and long-range chromosomal phase inference. Multivariable Cox regression models were used to ascertain the associations of mCAs. Exploratory endpoints included various incident cardiovascular phenotypes.
Results: Overall, 10,070 individuals (20.6%) carried ≥1 mCA clone. In adjusted analyses, mCA was associated with an increased risk of death of CAD causes (HR: 1.37, 95% CI: 1.09-1.71, P=0.006). In sub-analyses, we found that carriers of mCAs diagnosed with kidney cancer had an increased risk of death of CVD causes (HR: 2.03, 95% CI: 1.11-3.72, P=0.022) and CAD causes (HR: 3.57, 95% CI: 1.44-8.84, P=0.006). Women diagnosed with breast cancer who carried a mCA also had a higher risk of death of CAD causes (HR: 2.46, 95% CI: 1.23-4.92, P=0.011).
Conclusions: Among cancer survivors, carriers of any mCA are at an increased risk of CAD death compared to non-carriers. Mechanistic studies should be considered to better ascertain the biological mechanisms underneath the observed associations between mCAs and cardiovascular events for specific cancer types.
Impact: There may be clinical relevance in considering mCAs in patients diagnosed with cancer and undergoing treatment.
Supplementary Table S1 from KIT and PDGFRA Mutations and Survival of Gastrointestinal Stromal Tumor Patients Treated with Adjuvant Imatinib in a Randomized Trial
Authors: Joensuu Heikki , Wardelmann Eva , Eriksson Mikael , Reichardt Annette , Hall Kirsten Sundby , Schütte Jochen , Cameron Silke , Hohenberger Peter , Sihto Harri , Jost Philipp J. , Lindner Lars H. , Bauer Sebastian , Nilsson Bengt , Kallio Raija , Pesonen Tommi , Reichardt Peter
Published: April 2023

Representativeness of Study Participants
Supplementary Table S1 from A Tumor-suppressive Molecular Axis EP300/circRERE/miR-6837-3p/MAVS Activates Type I IFN Pathway and Antitumor Immunity to Suppress Colorectal Cancer
Authors: Ding Nan , You A-Bin , Yang Hu , Hu Guo-Sheng , Lai Chun-Ping , Liu Wen , Ye Feng
Published: April 2023

Table S1: Cloning sequence or targeting sequence used in this article.
Supplementary Figure S6 from A Tumor-suppressive Molecular Axis EP300/circRERE/miR-6837-3p/MAVS Activates Type I IFN Pathway and Antitumor Immunity to Suppress Colorectal Cancer
Authors: Ding Nan , You A-Bin , Yang Hu , Hu Guo-Sheng , Lai Chun-Ping , Liu Wen , Ye Feng
Published: April 2023

Figure S6: A proposed model of how circRERE functions as a tumor suppressor in CRC.
Supplementary Figure S2 from KIT and PDGFRA Mutations and Survival of Gastrointestinal Stromal Tumor Patients Treated with Adjuvant Imatinib in a Randomized Trial
Authors: Joensuu Heikki , Wardelmann Eva , Eriksson Mikael , Reichardt Annette , Hall Kirsten Sundby , Schütte Jochen , Cameron Silke , Hohenberger Peter , Sihto Harri , Jost Philipp J. , Lindner Lars H. , Bauer Sebastian , Nilsson Bengt , Kallio Raija , Pesonen Tommi , Reichardt Peter
Published: April 2023

Kaplan-Meier estimates of recurrence-free survival of patients with KIT exon 11 substitution mutation (A) and patients with KIT exon 9 mutation (B). The 5-year and 10-year survival rates are shown. Censored patients are indicated with a bar
Supplementary Figure S1 from KIT and PDGFRA Mutations and Survival of Gastrointestinal Stromal Tumor Patients Treated with Adjuvant Imatinib in a Randomized Trial
Authors: Joensuu Heikki , Wardelmann Eva , Eriksson Mikael , Reichardt Annette , Hall Kirsten Sundby , Schütte Jochen , Cameron Silke , Hohenberger Peter , Sihto Harri , Jost Philipp J. , Lindner Lars H. , Bauer Sebastian , Nilsson Bengt , Kallio Raija , Pesonen Tommi , Reichardt Peter
Published: April 2023

Kaplan-Meier estimates of recurrence-free survival (A, C) and overall survival (B, D) of patients with KIT exon 11 deletion mutation (A, B) and patients with KIT exon 11 indel mutation (C, D)
Data from KIT And PDGFRA Mutations And Survival of Gastrointestinal Stromal Tumor Patients Treated With Adjuvant Imatinib in a Randomized Trial
Authors: Joensuu Heikki , Wardelmann Eva , Eriksson Mikael , Reichardt Annette , Sundby Hall Kirsten , Schütte Jochen , Cameron Silke , Hohenberger Peter , Sihto Harri , Jost Philipp J. , Lindner Lars H. , Bauer Sebastian , Nilsson Bengt , Kallio Raija , Pesonen Tommi , Reichardt Peter
Published: April 2023

Abstract
Backgroud: Limited data are available about influence of KIT and PDGFRA mutations on overall survival (OS) of patients with gastrointestinal stromal tumor (GIST) treated with adjuvant imatinib. Patients and Methods: The Scandinavian Sarcoma Group XVIII/AIO multicenter trial accrued 400 patients with a high risk for GIST recurrence after macroscopically complete surgery between February 4, 2004 and September 29, 2008. The patients received adjuvant imatinib 400 mg/day for either 1 year or 3 years based on random allocation. We analyzed using conventional sequencing KIT and PDGFRA mutations centrally from 341 (85%) patients who had localized, centrally confirmed GIST, and correlated the results with recurrence-free survival (RFS) and OS in exploratory analyses. Results: During a median follow-up time of 10 years 164 RFS events and 76 deaths occurred. Most patients were re-treated with imatinib when GIST recurred. Patients with KIT exon 11 deletion or indel mutation treated with 3 years of adjuvant imatinib survived longer than patients treated for 1 year (10-year OS 86% versus 64%, respectively; hazard ratio [HR] 0.34, 95% CI: 0.15-0.72, P = 0.007), and had also longer RFS (10-year RFS 47% versus 29%; HR 0.48, 95% CI: 0.31-0.74, P < 0.001). Patients with KIT exon 9 mutation had unfavorable OS regardless of the duration of adjuvant imatinib. Conclusions: Compared to 1 year of imatinib, 3 years of adjuvant imatinib led to 66% reduction in the estimated risk of death and a high 10-year OS rate in the subset of patients with a KIT exon 11 deletion/indel mutation.
Supplementary Table 13 from Disruption of the MYC Superenhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid Leukemia
Authors: Yashar William M. , Curtiss Brittany M. , Coleman Daniel J. , VanCampen Jake , Kong Garth , Macaraeg Jommel , Estabrook Joseph , Demir Emek , Long Nicola , Bottomly Daniel , McWeeney Shannon K. , Tyner Jeffrey W. , Druker Brian J. , Maxson Julia E. , Braun Theodore P.
Published: April 2023

Excess over Bliss of Primary AML Blasts from 72 Patient Samples
Supplementary Fig. 5 from Disruption of the MYC Superenhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid Leukemia
Authors: Yashar William M. , Curtiss Brittany M. , Coleman Daniel J. , VanCampen Jake , Kong Garth , Macaraeg Jommel , Estabrook Joseph , Demir Emek , Long Nicola , Bottomly Daniel , McWeeney Shannon K. , Tyner Jeffrey W. , Druker Brian J. , Maxson Julia E. , Braun Theodore P.
Published: April 2023

STAT5 and MYC play a key role in the response to FLT3/LSD1 inhibition.
Supplementary Fig. 12 from Disruption of the MYC Superenhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid Leukemia
Authors: Yashar William M. , Curtiss Brittany M. , Coleman Daniel J. , VanCampen Jake , Kong Garth , Macaraeg Jommel , Estabrook Joseph , Demir Emek , Long Nicola , Bottomly Daniel , McWeeney Shannon K. , Tyner Jeffrey W. , Druker Brian J. , Maxson Julia E. , Braun Theodore P.
Published: April 2023

Dual FLT3/LSD1 inhibition disrupts LSC clonogenicity.
Supplementary Fig. 11 from Disruption of the MYC Superenhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid Leukemia
Authors: Yashar William M. , Curtiss Brittany M. , Coleman Daniel J. , VanCampen Jake , Kong Garth , Macaraeg Jommel , Estabrook Joseph , Demir Emek , Long Nicola , Bottomly Daniel , McWeeney Shannon K. , Tyner Jeffrey W. , Druker Brian J. , Maxson Julia E. , Braun Theodore P.
Published: April 2023

Single cell ATAC-seq cell population dynamics in primary patient samples follow-ing treatment with dual FLT3/LSD1 inhibition.
Data from EIF4A3 Acts on the PI3K–AKT–ERK1/2–P70S6K Pathway through FLOT1 to Influence the Development of Lung Adenocarcinoma
Authors: Yu Wenhao , Liang Jinghui , Fang Tao , Jiang Jin , Zhao Renchang , Li Rongyang , Han Jingyi , Tian Hui
Published: April 2023

Abstract
Lung adenocarcinoma (LUAD) is a major lung cancer subtype. In this study, we discovered that the eukaryotic translation initiation factor EIF4A3 expression was significantly higher in LUAD tissues and that this higher expression was closely linked to a poor prognosis for LUAD. In addition, we demonstrated that the knockdown of EIF4A3 significantly inhibited the proliferation, invasion, and migration of LUAD cells in vitro and in vivo. The findings of mass spectrometry analysis revealed that EIF4A3 could interact with Flotillin-1 in LUAD cells and that EIF4A3 could positively regulate the expression of FLOT1 at the protein level. Meanwhile, transcriptome sequencing showed that EIF4A3 could influence the development of LUAD by affecting PI3K–AKT–ERK1/2–P70S6K and PI3K class III–mediated autophagy in the Apelin pathway. In addition, we confirmed that Flotillin-1 expression was upregulated in LUAD based on the existing literature, and knockdown of FLOT1 could inhibit the proliferation and migration of LUAD cells. In addition, the knockdown of Flotillin-1 reversed the increase of cell proliferation and migration caused by EIF4A3 overexpression. Furthermore, we found that the activation of PI3K–AKT–ERK1/2–P70S6K signaling pathway and PI3K class III–mediated autophagy caused by EIF4A3 overexpression was rescued by the knockdown of FLOT1. In a word, we proved that EIF4A3 positively regulates the expression of FLOT1 and plays a procancer role in LUAD.
Implications:
Our study revealed the role of EIF4A3 in prognosis and tumor progression in LUAD, indicating that EIF4A3 could be used as the molecular diagnostic and prognostic therapeutic target.
Data from Non-essential amino acid availability influences proteostasis and breast cancer cell survival during proteotoxic stress
Authors: Sannino Sara , Manuel Allison M. , Shang Chaowei , Wendell Stacy G. , Wipf Peter , Brodsky Jeffrey L.
Published: April 2023

Abstract
Protein homeostasis (proteostasis) regulates tumor growth and proliferation when cells are exposed to proteotoxic stress, such as during treatment with certain chemotherapeutics. Consequently, cancer cells depend to a greater extent on stress signaling, and require the integrated stress response (ISR), amino acid metabolism, and efficient protein folding and degradation pathways to survive. To define how these interconnected pathways are wired when cancer cells are challenged with proteotoxic stress, we investigated how amino acid abundance influences cell survival when Hsp70, a master proteostasis regulator, is inhibited. We previously demonstrated that cancer cells exposed to a specific Hsp70 inhibitor induce the ISR via the action of two sensors, GCN2 and PERK, in stress-resistant and sensitive cells, respectively. In resistant cells, the induction of GCN2 and autophagy supported resistant cell survival, yet the mechanism by which these events were induced remained unclear. We now report that amino acid availability reconfigures the proteostasis network. Amino acid supplementation, and in particular arginine addition, triggered cancer cell death by blocking autophagy. Consistent with the importance of amino acid availability, which when limited activates GCN2, resistant cancer cells succumbed when challenged with a potentiator for another amino acid sensor, mTORC1, in conjunction with Hsp70 inhibition.
Implications: These data position amino acid abundance, GCN2, mTORC1, and autophagy as integrated therapeutic targets whose coordinated modulation regulates the survival of proteotoxic-resistant breast cancer cells.
Supplementary Table ST1 from Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer
Authors: Lang Liwei , Chen Fanghui , Li Yamin , Shay Chloe , Yang Fan , Dan Hancai , Chen Zhuo G. , Saba Nabil F. , Teng Yong
Published: April 2023

The primer sequences for qRT-PCR assays.
Supplementary Figure S9 from Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer
Authors: Lang Liwei , Chen Fanghui , Li Yamin , Shay Chloe , Yang Fan , Dan Hancai , Chen Zhuo G. , Saba Nabil F. , Teng Yong
Published: April 2023

Elevated PLXDC2 expression in radioresistant HN6 cells is mediated by c-Met-activated ERK1/2-ELK signaling.
Continuous Renal Replacement Therapy during Extracorporeal Membrane Oxygenation: Circuit Haemodynamics and Circuit Failure
Authors: Sansom Benjamin , Riley Brooke , Udy Andrew , Sriram Shyamala , Presneill Jeffrey , Bellomo Rinaldo
Published: April 2023

Introduction: Treatment with continuous renal replacement therapy (CRRT) is common during extracorporeal membrane oxygenation (ECMO). Such ECMO-CRRT has specific technical characteristics, which may affect circuit life. Accordingly, we studied CRRT haemodynamics and circuit life during ECMO. Methods: ECMO and non-ECMO-CRRT treatments in two adult intensive care units were compared using data collected over a 3-year period. A potential predictor of circuit survival identified in a 60% training data subset as a time-varying covariate within a Cox proportional hazard model was subsequently assessed in the complementary remaining data (40%). Results: Median [interquartile range] CRRT circuit life was greater when associated with ECMO (28.8 [14.0–65.2] vs. 20.2 [9.8–40.2] h, p < 0.0001). Access, return, prefilter, and effluent pressures were also greater during ECMO. Higher ECMO flows were associated with higher access and return pressures. Classification and regression tree analysis identified an association between high access pressures and accelerated circuit failure, while both first access pressures ≥190 mm Hg (HR 1.58 [1.09–2.30]) and patient weight (HR 1.85 [1.15–2.97] third tertile vs. first tertile) were independently associated with circuit failure in a multivariable Cox model. Access dysfunction was associated with a stepwise increase in transfilter pressure, suggesting a potential mechanism of membrane injury. Conclusion: CRRT circuits used in conjunction with ECMO have a longer circuit life than usual CRRT despite exposure to higher circuit pressures. Markedly elevated access pressures, however, may predict early CRRT circuit failure during ECMO, possibly via progressive membrane thrombosis as evidenced by increased transfilter pressure gradients.
Leisure Activities, Genetic Risk, and Frailty: Evidence from the Chinese Adults Aged 80 Years or Older
Authors: Zhou Jinhui , Li Xinwei , Gao Xiang , Wei Yuan , Ye Lihong , Liu Sixin , Ye Jiaming , Qiu Yidan , Zheng Xulin , Chen Chen , Wang Jun , Kraus Virginia Byers , Lv Yuebin , Mao Chen , Shi Xiaoming
Published: April 2023

Introduction: About half of adults aged ≥80 years suffer from frailty. Exercise is considered effective in preventing frailty but may be inapplicable to adults aged ≥80 years due to physical limitations. As an alternative, we aimed to explore the association of leisure activities with frailty and identify potential interaction with established polygenic risk score (PRS) among adults aged ≥80 years. Methods: Analyses were performed in a prospective cohort study of 7,471 community-living older adults aged ≥80 years who were recruited between 2002 and 2014 from 23 provinces in China. Leisure activity was assessed using a seven-question leisure activity index and frailty was defined as a frailty index ≥0.25 using a validated 39-item health-related scale. The PRS was constructed using 59 single-nucleotide polymorphisms associated with frailty in a subsample of 2,541 older adults. Cox proportional hazards models were used to explore the associations of leisure activities, PRS with frailty. Results: The mean age of participants was 89.4 ± 6.6 years (range: 80–116). In total, 2,930 cases of frailty were identified during 42,216 person-years of follow-up. Each 1 unit increase in the leisure activity index was associated with 12% lower risk of frailty (hazard ratio: 0.88 [95% confidence interval, 0.85–0.91]). Participants with high genetic risk (PRS >2.47 × 10−4) suffered from 26% higher risk of frailty. Interaction between leisure activity and genetic risk was not observed. Conclusion: Evidence is presented for the independent association of leisure activities and genetic risk with frailty. Engagement in leisure activities is suggested to be associated with lower risk of frailty across all levels of genetic risk among adults aged ≥80 years.
Fold, Twist, and Draw – Techniques of Copper Alloy Wire Production from Hellenistic Jebel Khalid
Authors: Mazis Matasha , Klein Matthias
Published: April 2023

Copper alloy wire fragments were examined using XRF, optical light microscopy and SEM-EDS. The specimens come from archaeological excavations at Jebel Khalid in Syria, dating from the 3rd century BCE (the Hellenistic period) to the Roman period. Our results show that several techniques were employed to make the wires: forging, folding, strip twisting, and possibly ‘strip drawing’. We investigated the morphologies, treatments, and fabrications attributed to making wire from copper alloys compared to more ductile materials such as gold and silver. Evidence of extensive annealing and non-uniform, sub-round profiles, and uneven and faceted surfaces represent the challenges of working with the material. There is no obvious evidence of solid wire drawing. The metalworkers used different copper alloys to make wire, some with high levels of lead (Pb). Subtle joins were observed in some samples, whereas others had evident folds and directional structures. The findings contribute new evidence to enhance our understanding of base-metal wire development in antiquity.
Non-invasive Archaeometric Studies of Metal Threads with Silk Core Coming from Two Kontush Sashes from the Szczuczyn Excavations in Poland
Authors: Miazga Beata , Grupa Małgorzata
Published: April 2023

A kontush sash belonged to costly accessories of ceremonial clothes of Polish nobility. It is a belt made of leather, metal or soft textile, called a sash from the 18th century. In the 2nd half of the 17th century, there was a change in manufacturing technique and ornamentation compositions, and sashes adorned with metal threads appeared. Persian and Turkish belts were the most popular. They have been excavated as burial equipment at archaeological sites. Analyzing these objects is a unique research experience that complements our knowledge of girdle-making in the eighteenth century. The non-invasive archaeometric studies are a significant part of this research. The microscopic observations, X-ray fluorescence and SEM-EDS investigations were made of the metal threads from the textile sashes found in the crypts of Szczuczyn church (North-Eastern Poland).
Amanita stubbosa, a new non amatoxin nor phallotoxin-containing species within Amanita sect. Phalloideae (Amanitaceae, Agaricales), from West Africa
Authors: CODJIA JEAN EVANS I. , SARAWI SEPAS , OLOU BORIS A. , PIEPENBRING MEIKE , YANG ZHU L. , YOROU NOUROU S.
Published: April 2023

Amanita stubbosa, a new species in subclade III of A. sect. Phalloideae, is described from West Africa based on morphological and molecular evidence. The species is characterized by a white pileus covered with small white broadly adherent squamules or greyish to whitish erected, pyramidal, subconical, patchy universal veil, a slightly striate pileal margin, a marginate basal bulb, and broadly ellipsoid to ellipsoid, weakly amyloid basidiospores. Phylogenetic analyses based on three gene regions (nrLSU, RPB2, and TEF1-α) revealed that A. stubbosa is a distinct species closely related to A. bulbulosa. Moreover, A. stubbosa was reported to lack the most notorious toxins (amatoxins and phallotoxins). A detailed description, illustrations, and comparisons with morphologically and phylogenetically closely related species are provided.
The Histories of Well-Documented Maritime Cyclones as Portrayed by an Automated Tracking Method
Authors: Roebber Paul J. , Grise Kevin M. , Gyakum John R.
Published: April 2023

This study examines extratropical cyclone tracks, central pressure, and maximum intensification rates from a widely used automated cyclone tracking scheme and compares them with the manual tracking of five well-known North Atlantic cyclones whose histories are available in the refereed literature. The automated tracking scheme is applied to sea level pressure data from four different reanalyses of varying levels of sophistication to test the sensitivity of the results to input data resolution and quality. Further, we test the tracking scheme using lower-tropospheric vorticity obtained from the most recent reanalysis (ERA5) for four of these cyclone events. Substantial discrepancies in cyclone position, intensity, and maximum intensification rates exist between the manual tracking and the automated tracking and are not eliminated by using higher-resolution reanalyses or by “turning off” the spatial smoothing feature of the automated tracking scheme (needed to reduce spurious cyclone detections). The results point to a particular problem in detecting weaker and earlier stage cyclones and confirm findings from studies that have examined a broad range of cyclone tracking schemes for a range of reanalyses. Notably, this early cyclone stage often involves a smaller-scale secondary cyclogenesis or cyclone wave, which are detected by the automated scheme only after subsequent growth in the ensuing 6–12 h. It is known that these early stages are critical for a comprehensive understanding of rapid intensification events. A discussion of possible future solutions to this problem is presented.

Significance Statement

Because of the availability of large modern datasets portraying sea level pressure across the globe, meteorologists have turned to automated detection and tracking of midlatitude cyclones. Detection and tracking are of interest since these storm systems play an important role in weather and climate and potential changes in their location, frequency, and intensity are of considerable societal interest given climate change. This paper compares the results obtained from one commonly used automated tracking method with tracks obtained by human analysts. We find substantial discrepancies in cyclone position, intensity, and intensification rates and that these differences are not eliminated by using improved analyses. A discussion of possible future solutions is presented.
Audiovisual production in Brazil: Will its public support survive the new political scenario?
Authors: Jambeiro Barbosa Othon Fernando , de Morais Kátia Santos , Canesso Natacha Stefanini , Domingues da Silva Juliano Mendonça , Evangelista Milena Silvino
Published: April 2023

This article discusses public funding of the development of Brazilian audiovisual production and the main agents involved in its conception and implementation. Public policies impacted the total revenue of US$ 4.5 billion of the national audiovisual industry in 2014, representing approximately 0.45% of the national GDP and surpassing the percentage participation of sectors such as the pharmaceutical industry. This article addresses the question of whether this innovative Brazilian policy for promoting audiovisual production can be considered to be wellconsolidated, with a well-structured active production chain. It discusses how the policy originated, how it became institutionalized, and the contribution provided by those who played a key role in its development and implementation (2009–2016). The analysis draws on theories relating to the political economy of communication. The research methods and techniques adopted include analysis of data from Brazilian government agencies, exhibitors, professional and trade-union associations, and media groups.
The validity of causal claims with repeated measures designs: A within-study comparison evaluation of differences-in-differences and the comparative interrupted time series
Authors: Anglin Kylie L , Wong Vivian C , Wing Coady , Miller-Bains Kate , McConeghy Kevin
Published: April 2023

Modern policies are commonly evaluated not with randomized experiments but with repeated measures designs like difference-in-differences (DID) and the comparative interrupted time series (CITS). The key benefit of these designs is that they control for unobserved confounders that are fixed over time. However, DID and CITS designs only result in unbiased impact estimates when the model assumptions are consistent with the data at hand. In this paper, we empirically test whether the assumptions of repeated measures designs are met in field settings. Using a within-study comparison design, we compare experimental estimates of the impact of patient-directed care on medical expenditures to non-experimental DID and CITS estimates for the same target population and outcome. Our data come from a multi-site experiment that includes participants receiving Medicaid in Arkansas, Florida, and New Jersey. We present summary measures of repeated measures bias across three states, four comparison groups, two model specifications, and two outcomes. We find that, on average, bias resulting from repeated measures designs are very close to zero (less than 0.01 standard deviations; SDs). Further, we find that comparison groups which have pre-treatment trends that are visibly parallel to the treatment group result in less bias than those with visibly divergent trends. However, CITS models that control for baseline trends produced slightly more bias and were less precise than DID models that only control for baseline means. Overall, we offer optimistic evidence in favor of repeated measures designs when randomization is not feasible.
Development and Psychometric Evaluation of the IPV Internalized Stigma Scale
Authors: Brunton Robyn , Harris Keith M.
Published: April 2023

Self-stigma is the internalization of widespread and negative attitudes around a devalued attribute. Being a victim of intimate partner violence (IPV) is a stigmatized identity, with IPV self-stigma is a potential barrier to help seeking. The lack of an IPV self-stigma scale limits current measurement of this latent trait; this study sought to fill this gap. We developed the IPV Internalized Self-stigma Scale (IPVIS) by revising existing self-stigma and devaluation/discrimination measures and adding new items to fill perceived gaps. Using an online survey, a diverse sample ( N = 455, M = 39.51, SD = 12.03) with various relationship types (e.g., heterosexual, same-sex), IPV circumstances (e.g., male or female perpetrators/victims) and different gender and sexual identities was recruited. Participants first completed the item pool (44-items), followed by measures of IPV, anxiety, depression, social health, and self-efficacy with data analyzed using a multi-model approach (e.g., factor analysis, item response theory [IRT]). Factor analyses revealed one dominant factor; IRT analyses further refined the unidimensional item set. The final 11 items had high internal consistency, ω = .90, 95% CI [0.89, 0.91], and were highly informative with moderate to high discrimination levels. The IPVIS demonstrated measurement invariance by demographics, showing no differential item functioning by age groups, sex, residence (urban/suburban/rural), ethnicity (European/Caucasian vs. others), or relationship status (partnered/unpartnered). Initial validity examination revealed significant correlations between the IPVIS and related measures (e.g., depression, anxiety, social health). The IPVIS is suitable for research and has widespread clinical applicability. To the best of our knowledge, the IPVIS is the first scale developed that assesses IPV self-stigma inclusive of a diverse range of clients/participants, relationship types, and IPV circumstances.
Bio-inspired skeletal model and kinematics of humanoid spine and ribs
Authors: Nidhi  , Noble Sam , Sooraj VS
Published: April 2023

Development of humanoids with human like range of motion for spine and torso is of great research interest. Present article covers the construction of the humanoid spine and ribs by considering spine as a hyper redundant mechanism with ribs attached in the thoracic region. To demonstrate the joints and motion characteristics, a thorough discussion of the biomechanical aspects of the spine is provided. A three-segment hyper redundant mechanism is utilised to imitate the human mimetic spine. The study considers flexion-extension and lateral bending motions of the spine. In the proposed formulation, a piecewise constant curvature approximation could simulate spine motion during flexion-extension movement. A simplified configuration with each section of spine controlled by two cables for flexion-extension and two cables for lateral bending is adopted. Additionally, a three dimensional CAD model of proposed system is developed and simulated using ADAMS. The constant curvature model and ADAMS simulation results closely match the human range of motion.
Lipid Peroxidation Inhibitory and Cytotoxic Activities of Two Camellia Species Growing Wild in Vietnam
Authors: An Nguyen Thi Giang , Chau Dao Thi Minh , Huong Le Thi , Van Khoa Vu , Hung Nguyen Huy , Thao Do Thi , Trang Vo Thi Quynh , Dai Do Ngoc , Setzer William N.
Published: April 2023

Background

Leaves, flowers and young shoots of yellow Camellia species are often used as a substitute for green tea in Vietnam. Yellow Camellia species contain high levels of polyphenol compounds, and have shown activities such as antioxidant, anticancer, and cytotoxic.

Objectives

We qualitatively screened phytochemicals, quantified total polyphenols of 70% ethanol (EtOH) extracts of leaves, young shoots, and flowers of Camellia vuquangensis and Camellia hatinhensis in Ha Tinh Province, Vietnam. Furthermore, these extracts have been evaluated for lipid peroxidation inhibitory activities at the in vivo level in the BALB/c mouse model, and in vitro cytotoxic activities.

Materials and Methods

Chemical methods and thin-layer chromatographic (TLC) analysis were used for the qualitative screening of phytochemicals. High-performance liquid chromatography (HPLC) was used to determine total polyphenols. Cell survival was determined through optical density (OD) measured when the protein composition of the cells was stained with sulforhodamine B (SRB). Inhibition of lipid peroxidation was evaluated by determining the content of malondialdehyde (MDA), which is a product of membrane lipid peroxidation.

Results

The total polyphenol contents of all parts of the two species were comparable to that of green tea with values between 319.3 and 342.6 mg gallic acid equivalents (GAE/g) dry weight. Extracts of leaves, flowers of C. vuquangensis and C. hatinhensis showed strong lipid peroxidation inhibitory activities (IC50: 7.92–17.45 µg/mL) and moderate cytotoxic activities against cell lines HepG2, A549, MCF7, SK-Mel-2, HT-29, and AGS (IC50: 34.73–80.58 µg/mL).

Conclusion

These two tea species may be considered as herbal teas with various health benefits.
Comparison of distance education and in-person education in procedural sedation and analgesia: A randomized controlled trial
Authors: Takeuchi Shinya , Norii Tatsuya , Rivera Marisa , Homma Yosuke
Published: April 2023

Background:

Despite the increasing demand for procedural sedation and analgesia training, the number of available instructors remains limited.

Objective:

The aim of this study is to compare distance education with in-person learning for knowledge acquisition and participant satisfaction in procedural sedation and analgesia education.

Methods:

We conducted a randomized controlled trial comparing distance education with in-person learning in Japan on three occasions. Twenty participants per course were randomly divided into five groups with stratification by year of graduation. The groups were randomized to either a distance education group or an in-person group. We conducted pre- and post-testing of procedural sedation and analgesia knowledge and surveyed participants’ perceptions by using a scale ranging from 0 (not enough) to 100 (enough). Our primary outcomes were knowledge acquisition and the quality of discussion.

Results:

We included 48 healthcare professionals in the analysis. Forty-one participants (83.6%) were men and the median years of experience was 5 (interquartile range: 3–9). Both groups had similar knowledge acquisition with the pre-test and post-test score difference and reported similar scores on the quality of discussion with the exception of the clarity of audio quality (93 vs 100, p = 0.017).

Conclusion:

In a randomized trial of distance versus in-person learning for the sedation course, we did not observe statistically significant differences in knowledge acquisition and participant satisfaction between the two groups except for audio quality for the case discussion. Further efforts to improve quality for distance learning in procedural sedation and analgesia education are needed.
Inhibin-Positive “Cholangioblastic” Variant of Intrahepatic Cholangiocarcinoma: Report of 3 New Patients With Review of the Literature
Authors: Bakhshwin Ahmed , Lai Keith K. , Ammoun Alwalid , Friedman Kenneth , El Hag Mohamed
Published: April 2023

Cholangiocarcinoma is the second most common primary liver malignant neoplasm. It usually affects older individuals in their seventh decade of life with no gender predilection. Recently, a distinct subtype of cholangiocarcinoma has emerged with 2 proposed names: “cholangioblastic” and “solid tubulocystic.” This variant predominantly occurs in younger women who lack the common risk factors for patients diagnosed with cholangiocarcinomas, such as older age and chronic liver disease or cirrhosis. We describe 3 new patients with a cholangioblastic variant of intrahepatic cholangiocarcinoma. At the time of diagnosis, the patients were aged 19-, 46-, and 28-year-old; 2 females and 1 male (the 46-year-old). None of our patients had a history of chronic liver disease or known predisposing factors for liver tumors. Tumor size ranged from 2.3 to 23 cm in greatest dimension. Histological examination of these tumors demonstrated reproducible morphology characterized by trabecular, nested, and multicystic patterns with micro and macro follicles filled with eosinophilic material. The immunohistochemical profile showed that the tumor cells were positive for keratin 7, inhibin, synaptophysin, and albumin in situ hybridization, while negative for HepPar1, arginase, and INSM1. All tumors lacked conventional intrahepatic cholangiocarcinoma/adenocarcinoma morphology. We also review the literature and emphasize that neuroendocrine tumors should be recognized as a major diagnostic pitfall of this variant.
Pan-Cancer Study of the Prognosistic Value of Selenium Phosphate Synthase 1
Authors: Hao Jie , He Ao-Yue , Zhao Xu , Chen Xue-Qin , Liu Qi-Ling , Sun Na , Zhang Rong-Qiang , Li Ping-Ping
Published: April 2023

Objective: This study sought to determine the mean prognostic usefulness of seleniumphosphate synthase ( SEPHS1) by investigating its expression in 33 human malignancies and its relationship to tumor immunity.

Methods: The expression of selenophosphate synthase 1 ( SEPHS1) in 33 human malignant tumors was examined using the Genotype-Tissue Expression (GTEx), Cancer Genome Atlas (TCGA), and TIMER databases. Furthermore, the TCGA cohort was used to investigate relationships between SEPHS1 and immunological checkpoint genes (ICGs), tumor mutation burden (TMB), microsatellite instability (MSI), and DNA mismatch repair genes (MMRs). To establish independent risk factors and calculate survival probabilities for liver hepatocellular carcinoma (LIHC) and brain lower-grade glioma (LGG), Cox regression models and Kaplan-Meier curves were utilized. Eventually, the Genomics of Cancer Drug Sensitivity (GDSC) database was used to evaluate the drug sensitivity in LGG and LIHC patients with high SEPHS1 expression.

Results: Overall, in numerous tumor tissues, SEPHS1 was highly expressed, and it significantly linked with the prognosis of LGG, ACC, and LIHC ( P < .05). Furthermore, in numerous cancers, SEPHS1 expression was linked to tumor-infiltrating immune cells (TIICs), TMB, MSI, and MMRs. According to univariate and multivariate Cox analyses, SEPHS1 expression was significant for patients with LGG and LIHC.

Conclusion: High SEPHS1 expression has a better prognosis for LGG, while low SEPHS1 expression has a better prognosis for LIHC. Chemotherapy was advised for LGG patients, particularly for those with high SEPHS1 expression because it can predict how responsive patients will be to 5-Fluorouracil and Temozolomide. This interaction between SEPHS1 and chemoradiotherapy has a positive clinical impact and may be used as evidence for chemotherapy for LGG and LIHC patients.
Women evaluate ally men less positively and are less willing to work with them for gender equality when men deny their male privilege
Authors: Wiley Shaun , Park Jun Won , Catalina Natalie
Published: April 2023

Members of advantaged groups can play a role in addressing discrimination and inequality. Sometimes, members of advantaged groups are motivated to deny their group-based privilege, however. In two studies ( Ns = 196, 600), we examined how privilege denial affects disadvantaged group members’ (i.e., women’s) evaluations of advantaged group members (i.e., men) who engage in collective action to support equality. We sampled women attending colleges or universities in the United States and randomly assigned them to read about a man who attended a rally to support gender equality and either denied his male privilege, acknowledged it, or said nothing about his privilege at all. We found that women evaluated a man who attended a rally to support gender equality less positively and were less willing to work with him for gender equality if he denied his male privilege. This pattern was either fully (Study 1) or partly (Study 2) statistically mediated by women rating a man who denied his male privilege as less moral, an effect that was stronger among women who identified more highly as feminists. Our findings indicate that advantaged group allies who deny their group-based privilege diminish their potential contributions to movements for equality.
“I didn’t Notice that You Were Watching Me”: Exploring a User Acceptance Study to Conduct Cultural Domain Analysis Online During the COVID-19 Pandemic
Authors: Ackley Caroline , Rodriguez Diego Garcia , Villa Giovanni
Published: April 2023

This article explores the implementation process of a User Acceptance Study to evaluate the feasibility of conducting cultural domain analysis (CDA) online during the COVID-19 pandemic. We conducted 19 cultural domain analysis sessions involving three techniques: free listing, pile sorts, and rank ordering. A diverse set of participants were recruited to help assess requirements and needs that researching online involves. We found that conducting CDA online is a feasible research method that offers benefits such as generating large amounts of data, making participants feel comfortable joining sessions from a safe space, providing anonymity, reducing research costs such as time and travel, and eliciting large numbers of responses. We also identified several factors for consideration when implementing CDA online and provide recommendations for improvement, including the aesthetics of the digital software employed, user accessibility and digital literacy, participants’ environments, Internet connection, and online-specific ethical issues.
Semaglutide for the treatment of antipsychotic-associated weight gain in patients not responding to metformin – a case series
Authors: Prasad Femin , De Riddhita , Korann Vittal , Chintoh Araba F. , Remington Gary , Ebdrup Bjørn H. , Siskind Dan , Knop Filip Krag , Vilsbøll Tina , Fink-Jensen Anders , Hahn Margaret K. , Agarwal Sri Mahavir
Published: April 2023

Metformin is the currently accepted first-line treatment for antipsychotic-associated weight gain (AAWG). However, not all patients benefit from metformin. Glucagon-like peptide-1 receptor agonists (GLP1-RA) have shown promise in the management of obesity in the general population, with preliminary evidence supporting efficacy in AAWG. Semaglutide is a weekly injectable GLP-1RA which received recent approval for obesity management and noted superiority over other GLP-1RAs. This study explored the efficacy and tolerability of semaglutide in AAWG among individuals with severe mental illness. A retrospective chart review of patients treated with semaglutide in the Metabolic Clinic at the Center for Addiction and Mental Health (CAMH) between 2019 and 2021 was conducted. Patients failing a trial of metformin (<5% weight loss or continuing to meet criteria for metabolic syndrome) after 3 months at the maximum tolerated dose (1500–2000 mg/day) were initiated on semaglutide up to 2 mg/week. The primary outcome measure was a change in weight at 3, 6, and 12 months. Twelve patients on weekly semaglutide injections of 0.71 ± 0.47 mg/week were included in the analysis. About 50% were female; the average age was 36.09 ± 13.32 years. At baseline, mean weight was 111.4 ± 31.7 kg, BMI was 36.7 ± 8.2 kg/m2, with a mean waist circumference of 118.1 ± 19.3 cm. A weight loss of 4.56 ± 3.15 kg ( p < 0.001), 5.16 ± 6.27 kg ( p = 0.04) and 8.67 ± 9 kg ( p = 0.04) was seen at 3, 6, and 12 months, respectively, after initiation of semaglutide with relatively well-tolerated side-effects. Initial evidence from our real-world clinical setting suggests that semaglutide may be effective in reducing AAWG in patients not responding to metformin. Randomized control trials investigating semaglutide for AAWG are needed to corroborate these findings.