bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Generalized Cauchy Process on Modeling the Long-Range Dependence and Self-Similarity of Sea Surface Chlorophyll Using 23 years of Remote Sensing Data
Authors: He Junyu
Published: September 2021

Understanding the temporal characteristics of sea surface chlorophyll (SSC) is helpful for marine environmental management. This study chose 10 time series of remote daily sea surface chlorophyll products from the European Space Agency during the period from July 29, 1998 to December 31, 2020. A generalized Cauchy model was employed to capture the local and global behaviors of sea surface chlorophyll from a fractal perspective; the fractal dimension D measures the local similarity while the Hurst parameter H measures the global long-range dependence. The generalized Cauchy model was fitted to the empirical autocorrelation function values of each SSC series. The results showed that the sea surface chlorophyll was multi-fractal in both space and time with the D values ranging from 1.0000 to 1.7964 and H values ranging from 0.6757 to 0.8431. Specifically, regarding the local behavior, 9 of the 10 series had low D values (<1.5), representing weak self-similarity; on the other hand, regarding the global behavior, high H values represent strong long-range dependence that may be a general phenomenon of daily sea surface chlorophyll.
Mitochondrial Fragmentation and Dysfunction in Type IIx/IIb Diaphragm Muscle Fibers in 24-Month Old Fischer 344 Rats
Authors: Brown Alyssa D. , Davis Leah A. , Fogarty Matthew J. , Sieck Gary C.
Published: September 2021

Sarcopenia is characterized by muscle fiber atrophy and weakness, which may be associated with mitochondrial fragmentation and dysfunction. Mitochondrial remodeling and biogenesis in muscle fibers occurs in response to exercise and increased muscle activity. However, the adaptability mitochondria may decrease with age. The diaphragm muscle (DIAm) sustains breathing, via recruitment of fatigue-resistant type I and IIa fibers. More fatigable, type IIx/IIb DIAm fibers are infrequently recruited during airway protective and expulsive behaviors. DIAm sarcopenia is restricted to the atrophy of type IIx/IIb fibers, which impairs higher force airway protective and expulsive behaviors. The aerobic capacity to generate ATP within muscle fibers depends on the volume and intrinsic respiratory capacity of mitochondria. In the present study, mitochondria in type-identified DIAm fibers were labeled using MitoTracker Green and imaged in 3-D using confocal microscopy. Mitochondrial volume density was higher in type I and IIa DIAm fibers compared with type IIx/IIb fibers. Mitochondrial volume density did not change with age in type I and IIa fibers but was reduced in type IIx/IIb fibers in 24-month rats. Furthermore, mitochondria were more fragmented in type IIx/IIb compared with type I and IIa fibers, and worsened in 24-month rats. The maximum respiratory capacity of mitochondria in DIAm fibers was determined using a quantitative histochemical technique to measure the maximum velocity of the succinate dehydrogenase reaction (SDHmax). SDHmax per fiber volume was higher in type I and IIa DIAm fibers and did not change with age. In contrast, SDHmax per fiber volume decreased with age in type IIx/IIb DIAm fibers. There were two distinct clusters for SDHmax per fiber volume and mitochondrial volume density, one comprising type I and IIa fibers and the second comprising type IIx/IIb fibers. The separation of these clusters increased with aging. There was also a clear relation between SDHmax per mitochondrial volume and the extent of mitochondrial fragmentation. The results show that DIAm sarcopenia is restricted to type IIx/IIb DIAm fibers and related to reduced mitochondrial volume, mitochondrial fragmentation and reduced SDHmax per fiber volume.
Contributions of CaV1.3 Channels to Ca2+ Current and Ca2+-Activated BK Current in the Suprachiasmatic Nucleus
Authors: McNally Beth A. , Plante Amber E. , Meredith Andrea L.
Published: September 2021

Daily regulation of Ca2+– and voltage-activated BK K+ channel activity is required for action potential rhythmicity in the suprachiasmatic nucleus (SCN) of the hypothalamus, the brain's circadian clock. In SCN neurons, BK activation is dependent upon multiple types of Ca2+ channels in a circadian manner. Daytime BK current predominantly requires Ca2+ influx through L-type Ca2+ channels (LTCCs), a time when BK channels are closely coupled with their Ca2+ source. Here we show that daytime BK current is resistant to the Ca2+ chelator BAPTA. However, at night when LTCCs contribute little to BK activation, BK current decreases by a third in BAPTA compared to control EGTA conditions. In phase with this time-of-day specific effect on BK current activation, LTCC current is larger during the day. The specific Ca2+ channel subtypes underlying the LTCC current in SCN, as well as the subtypes contributing the Ca2+ influx relevant for BK current activation, have not been identified. SCN neurons express two LTCC subtypes, CaV1.2 and CaV1.3. While a role for CaV1.2 channels has been identified during the night, CaV1.3 channel modulation has also been suggested to contribute to daytime SCN action potential activity, as well as subthreshold Ca2+ oscillations. Here we characterize the role of CaV1.3 channels in LTCC and BK current activation in SCN neurons using a global deletion of CACNA1D in mouse (CaV1.3 KO). CaV1.3 KO SCN neurons had a 50% reduction in the daytime LTCC current, but not total Ca2+ current, with no difference in Ca2+ current levels at night. During the day, CaV1.3 KO neurons exhibited oscillations in membrane potential, and most neurons, although not all, also had BK currents. Changes in BK current activation were only detectable at the highest voltage tested. These data show that while CaV1.3 channels contribute to the daytime Ca2+ current, this does not translate into a major effect on the daytime BK current. These data suggest that BK current activation does not absolutely require CaV1.3 channels and may therefore also depend on other LTCC subtypes, such as CaV1.2.
Comparative Analyses of Phyllosphere Bacterial Communities and Metabolomes in Newly Developed Needles of Cunninghamia lanceolata (Lamb.) Hook. at Four Stages of Stand Growth
Authors: Sun Kun , Sun Honggang , Qiu Zonghao , Liu Qiang
Published: September 2021

Host-plant-associated bacteria affect the growth, vigor, and nutrient availability of the host plant. However, phyllosphere bacteria have received less research attention and their functions remain elusive, especially in forest ecosystems. In this study, we collected newly developed needles from sapling (age 5 years), juvenile (15 years), mature (25 years), and overmature (35 years) stands of Chinese fir [Cunninghamia lanceolata (Lamb.) Hook]. We analyzed changes in phyllosphere bacterial communities, their functional genes, and metabolic activity among different stand ages. The results showed that phyllosphere bacterial communities changed, both in relative abundance and in composition, with an increase in stand age. Community abundance predominantly changed in the orders Campylobacterales, Pseudonocardiales, Deinococcales, Gemmatimonadales, Betaproteobacteriales, Chthoniobacterales, and Propionibacteriales. Functional predictions indicated the genes of microbial communities for carbon metabolism, nitrogen metabolism, antibiotic biosynthesis, flavonoids biosynthesis, and steroid hormone biosynthesis varied; some bacteria were strongly correlated with some metabolites. A total of 112 differential metabolites, including lipids, benzenoids, and flavonoids, were identified. Trigonelline, proline, leucine, and phenylalanine concentrations increased with stand age. Flavonoids concentrations were higher in sapling stands than in other stands, but the transcript levels of genes associated with flavonoids biosynthesis in the newly developed needles of saplings were lower than those of other stands. The nutritional requirements and competition between individual trees at different growth stages shaped the phyllosphere bacterial community and host–bacteria interaction. Gene expression related to the secondary metabolism of shikimate, mevalonate, terpenoids, tocopherol, phenylpropanoids, phenols, alkaloids, carotenoids, betains, wax, and flavonoids pathways were clearly different in Chinese fir at different ages. This study provides an overview of phyllosphere bacteria, metabolism, and transcriptome in Chinese fir of different stand ages and highlights the value of an integrated approach to understand the molecular mechanisms associated with biosynthesis.
Suppression of Phytochrome-Interacting Factors Enhances Photoresponses of Seedlings and Delays Flowering With Increased Plant Height in Brachypodium distachyon
Authors: Hoang Quyen T. N. , Tripathi Sharanya , Cho Jae-Yong , Choi Da-Min , Shin Ah-Young , Kwon Suk-Yoon , Han Yun-Jeong , Kim Jeong-Il
Published: September 2021

Phytochromes are red and far-red photoreceptors that regulate plant growth and development under ambient light conditions. During phytochrome-mediated photomorphogenesis, phytochrome-interacting factors (PIFs) are the most important signaling partners that regulate the expression of light-responsive genes. However, the function of PIFs in monocots has not been studied well. In this study, using RNA interference (RNAi), we investigated the functions of BdPIL1 and BdPIL3, two PIF-like genes identified in Brachypodium distachyon, which are closely related to Arabidopsis PIF1 and PIF3. The expression of their genes is light-inducible, and both BdPIL1 and BdPIL3 proteins interact with phytochromes in an active form-specific manner. Transgenic Brachypodium seedlings with the RNAi constructs of BdPIL1 and BdPIL3 showed decreased coleoptile lengths and increased leaf growth when exposed to both red and far-red light. In addition, the transgenic plants were taller with elongated internodes than wild-type Bd21-3 plant, exhibiting late flowering. Moreover, RNA-seq analysis revealed downregulation of many genes in the transgenic plants, especially those related to the regulation of cell number, floral induction, and chlorophyll biosynthesis, which were consistent with the phenotypes of increased plant height, delayed flowering, and pale green leaves. Furthermore, we demonstrated the DNA-binding ability of BdPIL1 and BdPIL3 to the putative target promoters and that the DNA-binding was inhibited in the presence of phytochromes. Therefore, this study determines a molecular mechanism underlying phytochrome-mediated PIF regulation in Brachypodium, i.e., sequestration, and also elucidates the functions of BdPIL1 and BdPIL3 in the growth and development of the monocot plant.
The Physical Learning Environment of Online Distance Learners in Higher Education – A Conceptual Model
Authors: Ng Cheuk Fan
Published: September 2021

Online distance learning is offered not only in post-secondary distance education institutions but in traditional universities as well. With advances in mobile and wireless technologies, completing academic studies anywhere anytime should become feasible. Research in distance education and online learning has focused on computer-mediated communication, instructional design, learner characteristics, educational technology, and learning outcomes. However, little attention has been given to where exactly learners do their learning and studying and how the physical and social aspects of the physical environment within which the online learner is physically embedded (e.g., the home) supports and constrains learning activities. In this paper, the author proposes a conceptual model for understanding the role that the physical environment plays in online distance learning in higher education, drawing on theories and research in environmental psychology, online learning, telework and mobile work, and higher education. Several gaps in research are identified, and suggestions for future research are proposed.
The Influence of Cross-Linguistic Similarity and Language Background on Writing to Dictation
Authors: Iniesta Antonio , Rossi Eleonora , Bajo M. Teresa , Paolieri Daniela
Published: September 2021

This study used a word dictation task to examine the influence of a variety of factors on word writing production: cognate status (cognate vs. non-cognate words), orthographic (OS) and phonological similarity (PS) within the set of cognate words, and language learning background [late bilinguals (LBs) with academic literacy and formal instruction in English and Spanish, and heritage speakers (HSs) with academic literacy and formal instruction only in English]. Both accuracy and reaction times for the first key pressed by participants (indicating lexical access), and the time required to type the rest of the word after the first keypress (indicating sublexical processing) was assessed. The results revealed an effect of PS on the dictation task particularly for the first keypress. That is, cognates with high PS were processed faster than cognates with low PS. In contrast to reading studies in which PS only revealed a significant effect when the OS between languages was high (O+P+ vs. O+P−), in the dictation to writing task, the phonology had a more general effect across all conditions, regardless of the level of OS. On the other hand, OS tended to be more influential for typing the rest of the word. This pattern is interpreted as indicating the importance of phonology (and PS in cognates) for initial lexical retrieval when the input is aural. In addition, the role of OS and PS during co-activation was different between groups probably due to the participants’ linguistic learning environment. Concretely, HSs were found to show relatively lower OS effects, which is attributed to the greater emphasis on spoken language in their Spanish language learning experiences, compared to the formal education received by the LBs. Thus, the study demonstrates that PS can influence lexical processing of cognates, as long as the task demands specifically require phonological processing, and that variations in language learning experiences also modulate lexical processing in bilinguals.
Psychological Distress Among Cancer Patients During COVID-19 Pandemic in the World: A Systematic Review
Authors: Momenimovahed Zohre , Salehiniya Hamid , Hadavandsiri Fatemeh , Allahqoli Leila , Günther Veronika , Alkatout Ibrahim
Published: September 2021

Aim: Patients with malignancies, experience high rates of psychological distress. Fear of Corona-infection combined with the interruptions in some treatment programs might affect the psychological health of cancer patients. This review study was conducted to investigate the psychological distress among cancer patients during COVID-19 pandemic to offer system-adapted individual solutions.

Materials and methods: To identify the psychological distress of cancer patients, a comprehensive search was carried out in PubMed, Web of Science, and Scopus. English language and original articles were included in this study. Articles that addressed any psychological distress among cancer patients during COVID-19 pandemic were included.

Results: At first 1,410 articles, were included in the study. After removing duplicate articles and reviewing the title and abstract, 55 articles were selected for the review. The findings of this study revealed COVID-19 greatly affects psychological health of cancer patients. Fear of COVID-19, fear of disease progression, disruption of oncology services, cancer stage, and immunocompromised status were the most common causes of psychological distress in oncology patients which can influence patients' decisions about treatment.

Conclusion: The COVID-19 related anxiety is an expected reaction to the current situation. Although psychological distress affects many people, it can confuse cancer patients to the point that they refuse to continue treatment for the fear of infection and worsening of their condition. Since the end of this pandemic is unknown, this action can endanger the health and prognosis of this group of patients, so it seems that using psychological interventions and intensive counseling in the current situation is one of the main priorities for cancer patients.
Young Children's Understanding of Restorative Justice
Authors: Zhou Zheng , Wong Wan-chi
Published: September 2021

The present study investigated how young children understand the sophisticated concept of restorative justice in unintentional moral transgressions. A sex-balanced sample of 5-year-old (M = 5.67, SD = 0.34, 49.3% girls) and 8-year-old (M = 7.86, SD = 0.29, 46.0% girls) Chinese children (N = 193) participated in the study. In designing the materials, we distilled the multidimensional meanings of restorative justice into two stories, one addressing the theme of property violation and the other physical harm; both stories were set in an animal community. We then engaged the children in joint reading and an interview, during which they showed preference for the given treatments for the transgressor (two restorative treatments vs. two retributive treatments) and ranked two further sets of restorative vs. retributive treatments at the community level. The results indicated that most children favored restorative treatments over retributive treatments for a transgressor, and the 8-year-olds viewed psychological restoration more favorably and behavioral punishment less favorably than the 5-year-olds. The children also tended to endorse restorative treatments at the community level, revealing an understanding of the needs, and obligations of all parties concerned. Notably, more 8- than 5-year-olds showed a consistency in restorative orientation at this level. Interpreting our data through the lens of the Representational Redescription model, we attained a more refined account of young children's levels of understanding regarding restorative justice. These results provide insights for the early cultivation of restorative justice among young children, which is a cornerstone for its successful practice in any society.
False Memories in Native and Foreign Languages
Authors: Dolgoarshinnaia Aleksandra , Martin-Luengo Beatriz
Published: September 2021

Human memory is prone to memory errors and distortion. Evidence from studies on cognitive functions in bilinguals indicates that they might be prone to different types of memory errors compared to monolinguals; however, the effect of language in false memories is still understudied. Source monitoring processes required for proper memory functioning, presumably, rely on inhibitory control, which is also heavily utilized by bilinguals. Moreover, it is suggested that thinking in a second language leads to more systematic and deliberate reasoning. All these results lead to expect that bilinguals are more analytical when processing information in their second language overcoming some memory errors depending on the language of information. To test this hypothesis, we run a classical misinformation experiment with an explicit source monitoring task with a sample of Russian–English bilinguals. The language of the misinformation presentation did not affect the degree of the misinformation effect between the Russian and English languages. Source monitoring demonstrated an overall higher accuracy for attributions to the English source over the Russian source. Furthermore, analysis on incorrect source attributions showed that when participants misattributed the sources of false information (English or Russian narrative), they favored the Russian source over the not presented condition. Taken together, these results imply that high proficiency in the second language does not affect misinformation and that information processing and memory monitoring in bilinguals can differ depending on the language of the information, which seems to lead to some memory errors and not others.
Satisfaction With Life, Satisfaction With Job, and the Level of Care Rationing Among Polish Nurses—A Cross-Sectional Study
Authors: Kołtuniuk Aleksandra , Witczak Izabela , Młynarska Agnieszka , Czajor Karolina , Uchmanowicz Izabella
Published: September 2021

Background: Rationing of nursing care is a serious issue that has been widely discussed throughout recent years in many countries. The level of satisfaction with life and of satisfaction with job as the nurse-related factors may significantly affect the level of care rationing.

Aim: To assess the rationing of nursing care among the Polish nurses and the impact of nurse-related variables, i.e., satisfaction with life and satisfaction with job on the level of nursing care rationing.

Materials and Methods: A cross-sectional study was conducted among 529 Polish registered nurses employing in two University Hospitals. Three self-report scales in the Polish version were used in this study, namely, Basel Extent of Rationing of Nursing Care-revised version (BERNCA-R), Satisfaction with Life Scale (SWLS), and Satisfaction with Work Scale (SWWS).

Results: The respondents indicated that the most frequently rationed activity is studying the situation of individual patients and care plans at the beginning of the shift. The least frequently rationed activity indicated by the respondents was adequate hand hygiene. The patient-to-nurse ratio and the level of satisfaction with job are significant independent factors affecting the level of care rationing.

Conclusions: The assessment of the level of satisfaction with life and identification of factors affecting this assessment will enable reducing the occurrence of care rationing.
Psychosocial Determinants of Marital Satisfaction Among Gynecologic Cancer Survivors in Malaysia
Authors: Hatta Sidi , Woon Luke Sy-Cherng , Nik Sumayyah Nik Mohd Nor , Mohamad Nasir Shafiee
Published: September 2021

Introduction: Marital issues among gynecologic cancer survivors are common but complex. This study aimed to investigate the relationship between sociodemographic and clinical factors, including sexual dysfunction and marital satisfaction among Malaysian gynecologic cancer survivors.

Methods: A cross-sectional survey of married women with gynecologic cancers was conducted at a Malaysian university hospital. Sociodemographic and clinical data were gathered. Sexual dysfunction was measured using the Malay Version Female Sexual Function Index (MVFSFI), while marital satisfaction was evaluated with the Malay Version Golombok Rust Inventory for Marital Satisfaction (MVGRIMS).

Results: A total of 116 patients participated in this study. The median age was 59.0 years (Interquartile range, IQR: 49.0–67.0 years); the median duration of marriage was 32 years (IQR: 20.0–40.8 years). 80.2% had a secondary and lower level of education. 37.9% of study subjects (n = 44) reported poor-and below-levels of marital satisfaction, which was equivalent to MVGRIMS transformed scores of >5. The median FSFI total score was 49.9 (IQR: 2.0–63.0). MVGRIMS transformed score correlated significantly with all MVFSFI sub-scores. In logistic regression, lower educational levels were associated with poor marital satisfaction [primary, (adjusted Odds Ratio) aOR = 12.67, 95% CI: 1.40–114.87; secondary: aOR = 11.52, 95% CI: 1.39–95.72], while higher MVFSFI total score reduced the likelihood of poor marital satisfaction (aOR = 0.979, 95% CI: 0.964–0.994).

Conclusion: Both sexual dysfunction and low education level may affect marital satisfaction among gynecologic cancer survivors. Targeted efforts focusing on sex education for patients may help to improve marital satisfaction.
The Response of Governments and Public Health Agencies to COVID-19 Pandemics on Social Media: A Multi-Country Analysis of Twitter Discourse
Authors: Li Lan , Aldosery Aisha , Vitiugin Fedor , Nathan Naomi , Novillo-Ortiz David , Castillo Carlos , Kostkova Patty
Published: September 2021

During the COVID-19 pandemic, information is being rapidly shared by public health experts and researchers through social media platforms. Whilst government policies were disseminated and discussed, fake news and misinformation simultaneously created a corresponding wave of “infodemics.” This study analyzed the discourse on Twitter in several languages, investigating the reactions to government and public health agency social media accounts that share policy decisions and official messages. The study collected messages from 21 official Twitter accounts of governments and public health authorities in the UK, US, Mexico, Canada, Brazil, Spain, and Nigeria, from 15 March to 29 May 2020. Over 2 million tweets in various languages were analyzed using a mixed-methods approach to understand the messages both quantitatively and qualitatively. Using automatic, text-based clustering, five topics were identified for each account and then categorized into 10 emerging themes. Identified themes include political, socio-economic, and population-protection issues, encompassing global, national, and individual levels. A comparison was performed amongst the seven countries analyzed and the United Kingdom (Scotland, Northern Ireland, and England) to find similarities and differences between countries and government agencies. Despite the difference in language, country of origin, epidemiological contexts within the countries, significant similarities emerged. Our results suggest that other than general announcement and reportage messages, the most-discussed topic is evidence-based leadership and policymaking, followed by how to manage socio-economic consequences.
Coffee With a Hint of Data: Towards Using Data-Driven Approaches in Personalised Long-Term Interactions
Authors: Irfan Bahar , Hellou Mehdi , Belpaeme Tony
Published: September 2021

While earlier research in human-robot interaction pre-dominantly uses rule-based architectures for natural language interaction, these approaches are not flexible enough for long-term interactions in the real world due to the large variation in user utterances. In contrast, data-driven approaches map the user input to the agent output directly, hence, provide more flexibility with these variations without requiring any set of rules. However, data-driven approaches are generally applied to single dialogue exchanges with a user and do not build up a memory over long-term conversation with different users, whereas long-term interactions require remembering users and their preferences incrementally and continuously and recalling previous interactions with users to adapt and personalise the interactions, known as thelifelong learningproblem. In addition, it is desirable to learn user preferences from a few samples of interactions (i.e.,few-shot learning). These are known to be challenging problems in machine learning, while they are trivial for rule-based approaches, creating a trade-off between flexibility and robustness. Correspondingly, in this work, we present the text-based Barista Datasets generated to evaluate the potential of data-driven approaches in generic and personalised long-term human-robot interactions with simulated real-world problems, such as recognition errors, incorrect recalls and changes to the user preferences. Based on these datasets, we explore the performance and the underlying inaccuracies of the state-of-the-art data-driven dialogue models that are strong baselines in other domains of personalisation in single interactions, namely Supervised Embeddings, Sequence-to-Sequence, End-to-End Memory Network, Key-Value Memory Network, and Generative Profile Memory Network. The experiments show that while data-driven approaches are suitable for generic task-oriented dialogue and real-time interactions, no model performs sufficiently well to be deployed in personalised long-term interactions in the real world, because of their inability to learn and use new identities, and their poor performance in recalling user-related data.
Exploring First-Person Perspectives in Designing a Role-Playing VR Simulation for Bullying Prevention: A Focus Group Study
Authors: Oyekoya Oyewole , Urbanski Jan , Shynkar Yaroslava , Baksh Arifa , Etsaghara Margaret
Published: September 2021

Bullying is a complex and abusive form of violence that has potentially serious social and mental health consequences for children and adolescents. With reference to the Olweus Bullying Circle, this project involves the development of a simulation that will allow students to view themselves in different roles played in bullying situations using a virtual reality setting. Interventions need to explore the perspective of the student who bullies and the student being bullied, as well as the bystander in order to model desirable intervention behavior. The expectation is that through role-playing, the students will explore different perspectives and learn how to respond to bullying situations. Two focus groups were conducted to allow experts to contribute to the design of the bullying prevention simulation and gather suggestions for improvements. Findings from the focus group studies suggest that to create effective bullying prevention, Virtual Reality simulations should consider focusing on role-playing, customization of the characters, environments, scenarios and a scoring/reward system.
Isothermal Amplification of Nucleic Acids: The Race for the Next “Gold Standard”
Authors: Oliveira Beatriz B. , Veigas Bruno , Baptista Pedro Viana
Published: September 2021

Nucleic acid amplification technologies (NAATs) have become fundamental tools in molecular diagnostics, due to their ability to detect small amounts of target molecules. Since its development, Polymerase Chain Reaction (PCR) has been the most exploited method, being stablished as the “gold standard” technique for DNA amplification. However, the requirement for different working temperatures leads to the need of a thermocycler machine or complex thermal apparatus, which have been preventing its application in novel integrated devices for single workflow and high throughput analysis. Conversely, isothermal amplification methods have been gaining attention, especially for point-of-care diagnosis and applications. These non-PCR based methods have been developed by mimicking the in vivo amplification mechanisms, while performing the amplification with high sensitivity, selectivity and allowing for high-throughput analysis. These favorable capabilities have pushed forward the implementation and commercialization of several platforms that exploit isothermal amplification methods, mostly against virus, bacteria and other pathogens in water, food, environmental and clinical samples. Nevertheless, the future of isothermal amplification methods is still dependent on achieving technical maturity and broader commercialization of enzymes and reagents.
Longer Disciplined Tapers Improve Marathon Performance for Recreational Runners
Authors: Smyth Barry , Lawlor Aonghus
Published: September 2021

For marathoners the taper refers to a period of reduced training load in the weeks before race-day. It helps runners to recover from the stresses of weeks of high-volume, high-intensity training to enhance race-day performance. The aim of this study was to analyse the taper strategies of recreational runners to determine whether particular forms of taper were more or less favorable to race-day performance.

Methods: We analyzed the training activities of more than 158,000 recreational marathon runners to define tapers based on a decrease in training volume (weekly distance). We identified different types of taper based on a combination of duration (1–4 weeks of decreasing training) and discipline (strict tapers progressively decrease training in the weeks before the marathon, relaxed tapers do not) and we grouped runners based on their taper type to determine the popularity of different types of taper and their associated performance characteristics.

Results: Kruskal-Wallis tests (H(7)≥ 521.11, p < 0.001), followed by posthoc Dunns tests with a Bonferroni correction, confirmed that strict tapers were associated with better marathon performance than relaxed tapers (p < 0.001) and that longer tapers of up to 3 weeks were associated with better performance than shorter tapers (p < 0.001). Results indicated that strict 3-week tapers were associated with superior marathon finish-time benefits (a median finish-time saving of 5 min 32.4 s or 2.6%) compared with a minimal taper (p < 0.001). We further found that female runners were associated with greater finish-time benefits than men, for a given taper type ( ≤ 3-weeks in duration), based on Mann Whitney U tests of significance with p < 0.001.

Conclusion: The findings of this study for recreational runners are consistent with related studies on highly-trained athletes, where disciplined tapers were associated with comparable performance benefits. The findings also highlight how most recreational runners (64%) adopt less disciplined (2-week and 3-week) tapers and suggest that shifting to a more disciplined taper strategy could improve performance relative to the benefits of a less disciplined taper.
Biomarkers in Bladder Cancer Surveillance
Authors: Sugeeta Sukumar S. , Sharma Anand , Ng Kenrick , Nayak Arvind , Vasdev Nikhil
Published: September 2021

Aim: This is a narrative review with an aim to summarise and describe urinary biomarkers in the surveillance of non-muscle-invasive bladder cancer (NMIBC). It provides a summary of FDA-approved protein biomarkers along with emerging ones which utilise genetic, epigenetic and exosomal markers. We discuss the current limitations of the available assays.

Background: Current guidelines advice a combination of cystoscopy, imaging,and urine cytology in diagnosis and surveillance. Although cytology has a high specificity, it is limited by low sensitivity particularly in low grade tumours. There are six FDA-approved urinary assays for diagnosis and surveillance of bladder cancer. They have shown to improve sensitivity and specificity to be used alongside cytology and cystoscopy but have a lower specificity in comparison to cytology and false positives often occur in benign conditions. Recent developments in laboratory techniques has allowed for use of markers which are RNA-, DNA-based as well as extracellular vesicles in the past decade.

Methods: Using the PubMed/Medline search engines as well as Google Scholar, we performed an online search using the terms “bladder cancer,” “non-muscle invasive bladder cancer,” and “urine biomarkers” with filter for articles in English published up to May 2021. Systematic reviews and original data of clinical trials or observational studies which contributed to the development of the biomarkers were collated.

Results: Biomarkers identified were divided into FDA-approved molecular biomarkers, protein biomarkers and gene-related biomarker with a table summarising the findings of each marker with the most relevant studies. The studies conducted were mainly retrospective. Due to the early stages of development, only a few prospective studies have been done for more recently developed biomarkers and limited meta-analyses are available.Therefore a detailed evaluation of these markers are still required to decide on their clinical use.

Conclusion: Advancements of analytical methods in BC has driven the research towards non-invasive liquid-based biomarkers in adjunct to urine cytology. Further large prospective studies are required to determine its feasibility in a clinical setting as they are not effective when used in isolation as they have their limitation. With the ongoing pandemic, other than reduction in costs and increased accuracy, the need for biomarkers to cope with delay in cystoscopies in diagnosis and surveillance is crucial. Thus clinical trials with direct comparison is required to improve patient care.
Low Expression of Stanniocalcin 1 (STC-1) Protein Is Associated With Poor Clinicopathologic Features of Endometrial Cancer
Authors: Khatun Masuma , Urpilainen Elina , Ahtikoski Anne , Arffman Riikka K. , Pasanen Annukka , Puistola Ulla , Tapanainen Juha S. , Andersson Leif C. , Butzow Ralf , Loukovaara Mikko , Piltonen Terhi T.
Published: September 2021

Stanniocalcin-1 (STC-1) is a glycoprotein hormone involved in diverse biological processes, including regulation of calcium phosphate homeostasis, cell proliferation, apoptosis, inflammation, oxidative stress responses, and cancer development. The role of STC-1 in endometrial cancer (EC) is yet to be elucidated. In this study, we investigated the protein expression pattern of STC-1 in a tissue microarray (TMA) cohort of hysterectomy specimens from 832 patients with EC. We then evaluated the prognostic value of STC-1 expression regarding the clinicopathologic features and patients survival over a period of 140 months. Our results revealed that in EC tissue samples, STC-1 is mainly localized in the endometrial epithelium, although some expression was also observed in the stroma. Decreased STC-1 expression was associated with factors relating to a worse prognosis, such as grade 3 endometrioid tumors (p = 0.030), deep myometrial invasion (p = 0.003), lymphovascular space invasion (p = 0.050), and large tumor size (p = 0.001). Moreover, STC-1 expression was decreased in tumors obtained from obese women (p = 0.014) and in women with diabetes mellitus type 2 (DMT2; p = 0.001). Interestingly, the data also showed an association between DNA mismatch repair (MMR) deficiency and weak STC-1 expression, specifically in the endometrial epithelium (p = 0.048). No association was observed between STC-1 expression and disease-specific survival. As STC-1 expression was particularly low in cases with obesity and DMT2 in the TMA cohort, we also evaluated the correlation between metformin use and STC-1 expression in an additional EC cohort that only included women with DMT2 (n = 111). The analysis showed no difference in STC-1 expression in either the epithelium or the stroma in women undergoing metformin therapy compared to metformin non-users. Overall, our data may suggest a favorable role for STC-1 in EC behavior; however, further studies are required to elucidate the detailed mechanism and possible applications to cancer treatment.
Information Fusion-Based Deep Neural Attentive Matrix Factorization Recommendation
Authors: Tian Zhen , Pan Lamei , Yin Pu , Wang Rui
Published: September 2021

The emergence of the recommendation system has effectively alleviated the information overload problem. However, traditional recommendation systems either ignore the rich attribute information of users and items, such as the user’s social-demographic features, the item’s content features, etc., facing the sparsity problem, or adopt the fully connected network to concatenate the attribute information, ignoring the interaction between the attribute information. In this paper, we propose the information fusion-based deep neural attentive matrix factorization (IFDNAMF) recommendation model, which introduces the attribute information and adopts the element-wise product between the different information domains to learn the cross-features when conducting information fusion. In addition, the attention mechanism is utilized to distinguish the importance of different cross-features on prediction results. In addition, the IFDNAMF adopts the deep neural network to learn the high-order interaction between users and items. Meanwhile, we conduct extensive experiments on two datasets: MovieLens and Book-crossing, and demonstrate the feasibility and effectiveness of the model.
Responses of Seed Yield Components to the Field Practices for Regulating Seed Yield of Smooth Bromegrass (Bromus inermis Leyss.)
Authors: Ou Chengming , Wang Mingya , Hou Longyu , Zhang Yangyang , Sun Ming , Sun Shoujiang , Jia Shangang , Mao Peisheng
Published: September 2021

Agronomic practices improve seed yield by regulating seed yield components, and the relationship between seed yield and seed yield components is still unclear in smooth bromegrass (Bromus inermis). To optimize seed production and yield in smooth bromegrass, a five-year field trial was designed with split-split-plot to study the combined effects of row spacing (30, 45, 60, and 75 cm), phosphorus (0, 60, 90, and 120 kg P ha−1) and nitrogen (0 and 100 kg N ha−1) on seed yield and seed yield components including fertile tillers m−2 (FTs), spikelets per fertile tiller (SFT), florets per spikelet (FS), and seeds per spikelet (SS). The results showed that FTs as a key factor had a positive effect to seed yield with the biggest pathway coefficient, while SS had a negative effect. Meanwhile, an interaction effect between FTs and SS was observed. FS and SS were increased with phosphorus application under the condition of sufficient nitrogen. In addition, sufficient precipitation at the non-growing season resulted in more FTs in the next year in rain-fed regions. Therefore, the optimum seed yield of smooth bromegrass can be obtained with row spacing (45 cm), nitrogen (100 kg N ha−1), and phosphorus application (60 kg P ha−1).
Does Digital Financial Inclusion Affect Agricultural Eco-Efficiency? A Case Study on China
Authors: Ma Jiehua , Li Zhenghui
Published: September 2021

Agricultural eco-efficiency can effectively reflect the coordinated green and balanced development of rural resources, and digital financial inclusion provides a strong financial power in the process of long-term balanced development of rural areas. There may be a complex relationship between the two. Therefore, based on the panel data of 30 provinces, autonomous regions, and municipalities in mainland China from 2011 to 2018, this paper explores the impact of digital financial inclusion on agricultural eco-efficiency through the differential GMM method. Further, the paper analyzes how digital financial inclusion influences agricultural eco-efficiency by influencing the agricultural scientific and technological investment. The following conclusions are drawn. First, there is a positive U-shaped nonlinear relationship between digital financial inclusion and agricultural eco-efficiency. Second, the impact of digital financial inclusion on agricultural eco-efficiency is of regional heterogeneity. Digital financial inclusion has a significant positive U-shaped impact on agricultural eco-efficiency in central China but has no significant impact on Eastern and Western China. Third, agricultural R&D investment will intensify the promotion effect of digital financial inclusion on agricultural eco-efficiency.
Extended-Spectrum β-Lactam Resistant Klebsiella pneumoniae and Escherichia coli in Wild European Hedgehogs (Erinaceus europeus) Living in Populated Areas
Authors: Garcias Biel , Aguirre Laia , Seminati Chiara , Reyes Nerea , Allepuz Alberto , Obón Elena , Molina-Lopez Rafael A. , Darwich Laila
Published: September 2021

Wildlife has been suggested to be a good sentinel of environmental health because of its close interaction with human populations, domestic animals, and natural ecosystems. The alarming emergence of antimicrobial resistance (AMR) in human and veterinary medicine has activated/triggered the awareness of monitoring the levels of AMR pollution in wildlife. European hedgehogs (Erinaceus europaeus) are common wild species habiting urban areas in Europe. However, there are few studies conducted in hedgehogs as reservoirs of AMR bacteria or genes. The aim of this study was to assess the occurrence and distribution of ESBL, AmpC, and carbapenem-resistant enterobacteria and AMR genes in wild European hedgehogs in Catalonia, a densely populated region of NE Spain. A total of 115 hedgehogs admitted at the Wildlife Rehabilitation Center of Torreferrussa were studied. To our knowledge, this is the first description of β-lactam resistant enterobacteria in wild hedgehogs. Interestingly, 36.8% (42/114) of the animals were detected as carriers of β-lactamase/carbapenemase resistance genes. Klebsiella spp. (59.6%), and specifically K. pneumoniae (84.6%), were the bacteria with the highest proportion of resistance genes, followed by E. coli (34.6%) and C. freundii (5.8%). The most frequently detected genetic variants were blaCTX-M-15 (19.3%), blaSHV-28 (10.5%), blaCMY-1 (9.7%), blaCMY-2 (8.8%), and blaOXA-48 (1.7%). In addition, 52% (27/52) of the isolates presented a multidrug resistance (MDR) phenotype and 31% had an extended drug resistance (XDR) profile. No clustering of animals with AMR genes within the study region was shown in the spatial analysis, nor differences in the proportion of positive animals among regions, were detected. The results of this study suggest that wild European hedgehogs could be good sentinels of AMR environmental pollution, especially in areas with a high human population density, because they either inhabit and/or feed in an anthropogenic environment. In conclusion, it is crucial to raise awareness of the strong interconnection between habitats and compartments, and therefore this implies that AMR issues must be tackled under the One Health approach.
Assessment of the Knowledge and Approach of General Dentists Who Treat Children and Pediatric Dentists Regarding the Proper Use of Antibiotics for Children
Authors: Rubanenko Moran , Blumer Sigalit , Maalof Kayan , Elbahary Shlomo , Katz Lazar , Kharouba Johnny
Published: September 2021

Background: Antibiotics are widely used in dentistry. Dentists often provide antibiotics unnecessarily. Excessive use can induce resistant bacterial strains. There are certain indications for the proper use of antibiotics for pediatric dentistry according to the European and American Pediatric Dentistry (EAPD and AAPD). Very often dentists do not follow these guidelines. Objectives: This study aims to examine the level of knowledge among general dentists (who also treat children) and pediatric dentists on proper use of antibiotics. In addition, we examined whether there is unjustified use of antibiotics, if dentists are aware of the new and conservative approach of administering antibiotics to patients, and whether there is a relationship between years of professional seniority and dentist’s knowledge level of proper use of antibiotics. Methods: One hundred general dentists (GD) who treat children in addition to 100 pediatric dentists (PD) completed the study questionnaires which measured knowledge, practice and attitudes regrading using antibiotics during dental treatment among children. Results: The general average of level of knowledge on proper use of antibiotics among general dentists was relatively low for both GD (60.7%) and PDs (65%). PDs demonstrated a relatively greater knowledge of correct use of antibiotics, especially in cases of endodontics and trauma. PDs also showed higher awareness to latest guidelines for the admission of “prophylactic antibiotics” according to the American Association (AAPD) and/or the European Union (EAPD) compared with GDs (86.2% vs. 66.3%). Conclusion: The level of knowledge of both general dentists and pediatric dentists is poor, with a large percentage of dentists from both groups not knowing whether antibiotics are needed in a specific dental case or not. Compliance with the EAPD/AAPD guidelines is also low and inadequate. The method of prescribing antibiotics given by dentists can be improved by increasing awareness, educational initiatives, and postgraduate courses among dentists regarding the recommended indications.