bluuebunny/crossref_metadata_2025 huggingface dataset:
Whole-Cell PVA Cryogel-Immobilized Microbial Consortium LE-C1 for Xanthan Depolymerization
Authors: Zhurishkina Elena V. , Eneyskaya Elena V. , Shvetsova Svetlana V. , Yurchenko Lyudmila V. , Bobrov Kirill S. , Kulminskaya Anna A.
Published: August 2023

Xanthan is an extracellular heteropolysaccharide produced by the bacteria Xanthomonas campestris. Due to its unique properties, the polysaccharide and its derivatives are widely used in many industries, from food to biomedicine and oil production, that demands an efficient xanthan depolymerization method to adapt this polysaccharide for various applications. Unlike the known chemical approaches, biological methods are considered to be more environmentally friendly and less energy intensive. In laboratory conditions, we have isolated a bacterial community capable of reducing the xanthan viscosity. Identification of the individual isolates in the microbial community and their testing resulted in the consortium LE-C1, consisting of two microorganisms Paenibacillus phytohabitans KG5 and Cellulosimicrobium cellulans KG3. The specific activities of the overall xanthanase and auxiliary enzymes that may be involved in the xanthan depolymerization were as follows: xanthanase, 19.6 ± 0.6 U/g; β-glucosidase, 3.4 ± 0.1 U/g; α-mannosidase, 68.0 ± 2.0 U/g; β-mannosidase, 0.40 ± 0.01 U/g; endo-glucanase, 4.0 ± 0.1 U/g; and xanthan lyase, 2.20 ± 0.07 U/mg. In order to increase the efficiency of xanthan biodegradation, the LE-C1 whole cells were immobilized in a poly(vinyl alcohol) cryogel. The resulting regenerative biocatalyst was able to complete xanthan depolymerization within 40 cycles without loss of activity or degradation of the matrix.
Visible Light Photocatalytic Degradation Performance of Metal (Fe, Ce, Ni, Mn, Bi)-Doped Sodium Tantalite Perovskite
Authors: Huang Aijun , Zhan Haijuan , Wen Meng , Zhou Yao , Bi Shuxian , Liu Wanyi , Li Feng
Published: August 2023

Metal ion doping is the most widely used means to improve the photocatalytic performance of semiconductor materials, which can adjust the band gap, broaden the range of optical response and construct impurity levels. The high efficiency modified NaTaO3 perovskite catalyst with good structural and catalytic properties was synthesized by a simple hydrothermal reaction method. A variety of analysis and testing techniques, such as XRD, SEM, DRS, XPS and EPR, were used to analyze the structure properties of the prepared materials. The results show that the influence mechanism of different metal introduction on the structure and properties of the NaTaO3 perovskite was different. Metal doping promoted the bond angle of Ta-O-Ta close to 180°, which restrains the recombination of the photogenerated electron-holes in the crystal. As Ce is introduced into the perovskite, the CeO2 forms and agglomerates around the perovskite, which improves the electron transport performance. With the narrower band gap, the Ce-modified perovskite shows that the degradation rate of ARS is 84% after 180 min of photoreaction. The species of h+, O2− and ·OH play different roles in improving the performance of the photocatalytic degradation process.
A Review: Methodologies to Promote the Differentiation of Mesenchymal Stem Cells for the Regeneration of Intervertebral Disc Cells Following Intervertebral Disc Degeneration
Authors: Ohnishi Takashi , Homan Kentaro , Fukushima Akira , Ukeba Daisuke , Iwasaki Norimasa , Sudo Hideki
Published: August 2023

Intervertebral disc (IVD) degeneration (IDD), a highly prevalent pathological condition worldwide, is widely associated with back pain. Treatments available compensate for the impaired function of the degenerated IVD but typically have incomplete resolutions because of their adverse complications. Therefore, fundamental regenerative treatments need exploration. Mesenchymal stem cell (MSC) therapy has been recognized as a mainstream research objective by the World Health Organization and was consequently studied by various research groups. Implanted MSCs exert anti-inflammatory, anti-apoptotic, and anti-pyroptotic effects and promote extracellular component production, as well as differentiation into IVD cells themselves. Hence, the ultimate goal of MSC therapy is to recover IVD cells and consequently regenerate the extracellular matrix of degenerated IVDs. Notably, in addition to MSC implantation, healthy nucleus pulposus (NP) cells (NPCs) have been implanted to regenerate NP, which is currently undergoing clinical trials. NPC-derived exosomes have been investigated for their ability to differentiate MSCs from NPC-like phenotypes. A stable and economical source of IVD cells may include allogeneic MSCs from the cell bank for differentiation into IVD cells. Therefore, multiple alternative therapeutic options should be considered if a refined protocol for the differentiation of MSCs into IVD cells is established. In this study, we comprehensively reviewed the molecules, scaffolds, and environmental factors that facilitate the differentiation of MSCs into IVD cells for regenerative therapies for IDD.
Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells
Authors: Djemai Mohammed , Cupelli Michael , Boutjdir Mohamed , Chahine Mohamed
Published: August 2023

Optical mapping is a powerful imaging technique widely adopted to measure membrane potential changes and intracellular Ca2+ variations in excitable tissues using voltage-sensitive dyes and Ca2+ indicators, respectively. This powerful tool has rapidly become indispensable in the field of cardiac electrophysiology for studying depolarization wave propagation, estimating the conduction velocity of electrical impulses, and measuring Ca2+ dynamics in cardiac cells and tissues. In addition, mapping these electrophysiological parameters is important for understanding cardiac arrhythmia mechanisms. In this review, we delve into the fundamentals of cardiac optical mapping technology and its applications when applied to hiPSC-derived cardiomyocytes and discuss related advantages and challenges. We also provide a detailed description of the processing and analysis of optical mapping data, which is a crucial step in the study of cardiac diseases and arrhythmia mechanisms for extracting and comparing relevant electrophysiological parameters.
Phage Endolysins: Advances in the World of Food Safety
Authors: Nazir Amina , Xu Xiaohui , Liu Yuqing , Chen Yibao
Published: August 2023

As antimicrobial resistance continues to escalate, the exploration of alternative approaches to safeguard food safety becomes more crucial than ever. Phage endolysins are enzymes derived from phages that possess the ability to break down bacterial cell walls. They have emerged as promising antibacterial agents suitable for integration into food processing systems. Their application as food preservatives can effectively regulate pathogens, thus contributing to an overall improvement in food safety. This review summarizes the latest techniques considering endolysins’ potential for food safety. These techniques include native and engineered endolysins for controlling bacterial contamination at different points within the food production chain. However, we find that characterizing endolysins through in vitro methods proves to be time consuming and resource intensive. Alternatively, the emergence of advanced high-throughput sequencing technology necessitates the creation of a robust computational framework to efficiently characterize recently identified endolysins, paving the way for future research. Machine learning encompasses potent tools capable of analyzing intricate datasets and pattern recognition. This study briefly reviewed the use of these industry 4.0 technologies for advancing the research in food industry. We aimed to provide current status of endolysins in food industry and new insights by implementing these industry 4.0 strategies revolutionizes endolysin development. It will enhance food safety, customization, efficiency, transparency, and collaboration while reducing regulatory hurdles and ensuring timely product availability.
Maternal Voice and Tactile Stimulation Modulate Oxytocin in Mothers of Hospitalized Preterm Infants: A Randomized Crossover Trial
Authors: Hirschel Jessica , Carlhan-Ledermann Audrey , Ferraz Céline , Brand Laure-Anne , Filippa Manuela , Gentaz Edouard , Lejeune Fleur , Baud Olivier
Published: August 2023

Prematurity is a major risk factor for perinatal stress and neonatal complications leading to systemic inflammation and abnormal mother–infant interactions. Oxytocin (OT) is a neuropeptide regulating the inflammatory response and promoting mother–infant bonding. The release of this hormone might be influenced by either vocal or tactile stimulation. The main objective of the current randomized, crossover, clinical trial was to assess the salivary OT/cortisol balance in mothers following the exposure of their baby born preterm to two types of sensorial interventions: maternal voice without or with contingent tactile stimulation provided by the mother to her infant. Among the 26 mothers enrolled, maternal voice intervention alone had no effect on OT and cortisol levels in the mothers, but when associated with tactile stimulation, it induced a significant increase in maternal saliva oxytocin (38.26 ± 30.26 pg/mL before vs 53.91 ± 48.84 pg/mL after, p = 0.02), particularly in the mothers who delivered a female neonate. Maternal voice intervention induced a significant reduction in cortisol and an increase in OT levels in mothers when the maternal voice with a tactile stimulation intervention was performed first. In conclusion, exposure to the maternal voice with a contingent tactile stimulation was associated with subtle changes in the maternal hormonal balance between OT and cortisol. These findings need to be confirmed in a larger sample size and may ultimately guide caregivers in providing the best intervention to reduce parental stress following preterm delivery.
Correlation of Speckle-Tracking Echocardiography with Traditional Biomarkers in Predicting Cardiotoxicity among Pediatric Hemato-Oncology Patients: A Comprehensive Evaluation of Anthracycline Dosages and Treatment Protocols
Authors: Ardelean Andrada Mara , Olariu Ioana Cristina , Isac Raluca , Jurac Ruxandra , Stolojanu Cristiana , Murariu Mircea , Toma Ana-Olivia , Braescu Laurentiu , Mavrea Adelina , Doros Gabriela
Published: August 2023

Speckle tracking-echocardiography (STE) is a novel non-invasive imaging tool capable of quantifying myocardial deformation, and thus holds promise in detecting early subclinical myocardial injury. This study aimed to evaluate the correlation of STE with traditional biomarkers in predicting anthracycline-induced cardiotoxicity in the context of varying dosages and treatment protocols in pediatric hemato-oncology patients. We conducted a retrospective study involving pediatric hemato-oncology patients undergoing anthracycline-based chemotherapy. A total of 99 patients were included in the final analysis, with 82 receiving Doxorubicin, of which 58.5% were males, and 17 receiving Epirubicin, of which 70.6% were males, with a median of 10 years old. Traditional biomarkers, such as Troponin I (cTnI) and B-type natriuretic peptide (BNP), were compared with STE parameters, including the global longitudinal strain (GLS), Simpson method of discs (SMOD), and myocardial performance index (MPI). A comprehensive evaluation was conducted based on different dosages of anthracyclines and different treatment protocols, with a follow-up period of one year post-chemotherapy. It was observed that the cTnI levels in the Doxorubicin group were significantly higher (3.2 ng/mL, p = 0.002) than in the Epirubicin group (2.7 ng/mL). However, BNP and NT-proBNP levels were not significantly different between the two groups (p = 0.096 and p = 0.172, respectively). Regarding STE parameters, a significant negative correlation was observed between the anthracycline dose and GLS (Rho = −0.411, p = 0.001), indicating increased cardiotoxicity with dose elevation. The SMOD and MPI gave significantly better values in the Epirubicin group (59.2 and 0.41 vs. 54.4 and 0.36, respectively). However, the ROC analysis did not find GLS, SMOD, or MPI to be significant independent predictors of cardiotoxicity (p > 0.05). There was also considerable variation in cardiotoxicity between the Doxorubicin and Epirubicin study groups, suggesting that the risk of cardiotoxicity is not solely determined by dose. Our study underlines the potential of STE as a sensitive tool for the early detection and prediction of anthracycline-induced cardiotoxicity in pediatric hemato-oncology patients, but only in association with the clinical findings and cardiac biomarkers. While traditional biomarkers still play a role, STE can offer a more accurate prediction of cardiac risk, potentially leading to better management and outcomes for these patients.
Evaluation of Gridded Meteorological Data for Crop Sensitivity Assessment to Temperature Changes: An Application with CERES-Wheat in the Mediterranean Basin
Authors: Liakopoulou Konstantina S. , Mavromatis Theodoros
Published: August 2023

In areas with a limited or non-existent network of observing stations, it is critical to assess the applicability of gridded datasets. This study examined the agreement of Agri4Cast and E-OBS at two spatial resolutions (10 km (EOBS-0.1) and 25 km (EOBS-0.25)) in 13 Mediterranean stations nearby to wheat crops and how this agreement may influence simulated potential development and production with the crop simulation model (CSM) CERES-Wheat in historical and near-future (2021–2040) (NF) periods. A wide range of sensitivity tests for maximum and minimum air temperatures and impact response surfaces were used for the future projections. EOBS-0.1 showed the lowest discrepancies over observations. It underestimated statistical measures of temperature and precipitation raw data and their corresponding extreme indices and overestimated solar radiation. These discrepancies caused small delays (5–6 days, on average) in crop development and overestimations (8%) in grain production in the reference period. In the NF, the use of EOBS-0.1 reduced by a few (2–3) days the biases in crop development, while yield responses differed among stations. This research demonstrated the ability of EOBS-0.1 for agricultural applications that depend on potential wheat development and productivity in historical and future climate conditions expected in the Mediterranean basin.
Sensitivity Analysis of Heat Stress Indices
Authors: Rachid Ahmed , Qureshi Aiman Mazhar
Published: August 2023

More than 40 heat indices are being used across the world to quantify outdoor thermal comfort. The selection of an Outdoor Heat Stress Index (OHSI) depends on several parameters, including clothing, age, awareness, local environment, food consumption, human activities, and resources. This study investigates various indicators of heat stress, including (i) OHSIs officially used to quantify heat stress worldwide, (ii) the estimation methods of these indices, and (iii) the sensitivity analysis of indices, namely, Corrected Effective Temperature (CET), Heat Index (HI), Wet Bulb Globe Temperature (WBGT), Universal Thermal Climate Index (UTCI), Discomfort Index (DI), Summer Simmer Index (SSI), and Predicted Mean Vote (PMV). The results indicate the degree of sensitivity of indices, with the HI being the most sensitive for estimating heat stress. Additionally, the WBGT, HI, and CET are recommended indices that can be directly measured using sensors instead of relying on calculated indices that are based on estimation techniques and some ideal physical assumptions.
Comparative Analysis of Performance of Water-Based Coatings Prepared by Two Kinds of Anti-Bacterial Microcapsules and Nano-Silver Solution on the Surface of Andoung Wood
Authors: Zou Yuming , Pan Pan , Yan Xiaoxing
Published: August 2023

A nano-silver solution, urea-formaldehyde resin-coated nano-silver solution microcapsules (AgNPS@UF), and melamine-formaldehyde resin-coated chitosan-modified nano-silver solution microcapsules (CS-AgNPS@MF) were added into the coatings at different contents for comparative tests to explore an anti-bacterial agent with the best anti-bacterial properties in the water-based coatings on Andoung wood (Monopetalanthus spp.). As the content of anti-bacterial agents increased, AgNPS@UF had the best anti-bacterial property towards Staphylococcus aureus and Escherichia coli, with anti-bacterial rates of 79.0% and 82.1%, respectively. The optical and mechanical properties of the coating quickly worsened as the content of anti-bacterial agents increased. The anti-bacterial coatings with AgNPS@UF have the minimum chromatic aberration change of 6.5. The anti-bacterial coating with 5% content of AgNPS@UF decreased the aging rate. This coating had good optical properties, and its liquid-resistant level is 2. When the 5% content of AgNPS@UF was added, after high-temperature accelerated aging and ultraviolet (UV) aging, the anti-bacterial rates of the coating with AgNPS@UF decreased toward Escherichia coli from 82.1% to 62.2% and decreased toward Staphylococcus aureus from 79.3% to 61.1%, respectively. This shows that the coating had a long-lasting anti-bacterial property. The anti-bacterial property of the coating after high-temperature accelerated aging was superior to that after the UV aging. The incorporation of AgNPS@UF in water-based coatings protects people from the risks of Escherichia coli and Staphylococcus aureus more effectively with long-lasting property. The research results provide a reference for the preparation of anti-bacterial water-based coatings on the surface of the Andoung wood.
Electrodeposited Fabrication of CeO2 Branched-like Nanostructure Used for Nonenzymatic Glucose Biosensor
Authors: Nguyet Nguyen Thi , Tuan Chu Van , Ngan Dang Thi Thuy , Tam Phuong Dinh , Nguyen Vinh Dinh , Nghia Nguyen Trong
Published: August 2023

The fabrication of nonenzymatic glucose sensors is essential because of the enhancement in the selectivity and accuracy of these sensors. In this work, we used the electrodeposition approach to prepare a CeO2-based electrode for nonenzymatic glucose detection. A CeO2 branched-like nanostructure was successfully fabricated by electrodeposition on the surface of a Au substrate electrode at room temperature. The effects of cyclic voltammetry, CH3COOH content, and scan cycle number on the formation of the CeO2 branched-like nanostructure were investigated. The fabricated electrodes were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The results showed that a CeO2 branched-like nanostructure could be obtained with a CH3COOH content of 1.0 mL and a scan cycle number of 100 in a solution containing 0.015 M Ce(NO3)3, 0.01 M KCl, and 0.02 M CH3COONH4 and with a scan rate of 400 mV/s. The electrochemical characteristics of the sensor were examined by chronoamperometry and cyclic voltammetry. The results showed that the sensitivity of the sensor was 37.72 μA/mM·cm2 and the limit of detection (LOD) of the sensor was 0.093 mM. The findings in this work prove that it is feasible to fabricate CeO2-based sensors for nonenzymatic glucose detection.
Enumeration of Self-Avoiding Random Walks on Lattices as Model Chains in Polymer Crystals
Authors: Benito Javier , Urrutia Unai , Karayiannis Nikos Ch. , Laso Manuel
Published: August 2023

Recent simulation studies have revealed a wealth of distinct crystal polymorphs encountered in the self-organization of polymer systems driven by entropy or free energy. The present analysis, based on the concept of self-avoiding random walks (SAWs) on crystal lattices, is useful to calculate upper bounds for the entropy difference of the crystals that are formed during polymer crystallization and thus to predict the thermodynamic stability of distinct polymorphs. Here, we compare two pairs of crystals sharing the same coordination number, ncoord: hexagonal close-packed (HCP) and face centered cubic (FCC), both having ncoord = 12 and the same packing density, and the less dense simple hexagonal (HEX) and body centered cubic (BCC) lattices, with ncoord = 8. In both cases, once a critical number of steps is reached, one of the crystals shows a higher number of SAWs compatible with its geometry. We explain the observed trends in terms of the bending and torsion angles as imposed by the geometric constraints of the crystal lattice.
Phase Structures, Electromechanical Responses, and Electrocaloric Effects in K0.5Na0.5NbO3 Epitaxial Film Controlled by Non-Isometric Misfit Strain
Authors: Wu Yingying , Ou Yun , Peng Jinlin , Lei Chihou
Published: August 2023

Environmentally friendly lead-free K1-xNaxNbO3 (KNN) ceramics possess electromechanical properties comparable to lead-based ferroelectric materials but cannot meet the needs of device miniaturization, and the corresponding thin films lack theoretical and experimental studies. To this end, we developed the nonlinear phenomenological theory for ferroelectric materials to study the effects of non-equiaxed misfit strain on the phase structure, electromechanical properties, and electrical response of K0.5Na0.5NbO3 epitaxial films. We constructed in-plane misfit strain (u1−u2) phase diagrams. The results show that K0.5Na0.5NbO3 epitaxial film under non-equiaxed in-plane strain can exhibit abundant phase structures, including orthorhombic a1c, a2c, and a1a2 phases, tetragonal a1, a2, and c phases, and monoclinic r12 phases. Moreover, in the vicinity of a2c−r12, a1c−c, and a1a2−a2 phase boundaries, K0.5Na0.5NbO3 epitaxial films exhibit excellent dielectric constant ε11, while at a2c−r12 and a1c−c phase boundaries, a significant piezoelectric coefficient d15 is observed. It was also found that high permittivity ε33 and piezoelectric coefficients d33 exist near the a2c−a2, a1a2−r12, and a1c−a1 phase boundaries due to the existence of polymorphic phase boundary (PPB) in the KNN system, which makes it easy to polarize near the phase boundaries, and the polarizability changes suddenly, leading to electromechanical enhancement. In addition, the results show that the K0.5Na0.5NbO3 thin films possess a large electrocaloric response at the phase boundary at the a1a2−r12 and a1c−a1 phase boundaries. The maximum adiabatic temperature change ΔT is about 3.62 K when the electric field change is 30 MV/m at room temperature, which is significantly enhanced compared with equiaxed strain. This study provides theoretical guidance for obtaining K1−xNaxNbO3 epitaxial thin films with excellent properties.
Nanocrystallization of Cu46Zr33.5Hf13.5Al7 Metallic Glass
Authors: Saini Jaskaran , Koledin Tamara , Thaiyanurak Tittaya , Chen Lei , Santala Melissa , Xu Donghua
Published: August 2023

The recently discovered Cu46Zr33.5Hf13.5Al7 (at.%) bulk metallic glass (BMG) presents the highest glass-forming ability (GFA) among all known copper-based alloys, with a record-breaking critical casting thickness (or diameter) of 28.5 mm. At present, much remains to be explored about this new BMG that holds exceptional promise for engineering applications. Here, we report our study on the crystallization behavior of this new BMG, using isochronal and isothermal differential scanning calorimetry (DSC), X-ray diffraction (XRD), and transmission electron microscopy (TEM). With the calorimetric data, we determine the apparent activation energy of crystallization, the Avrami exponent, and the lower branch of the isothermal time–temperature–transformation (TTT) diagram. With XRD and TEM, we identify primary and secondary crystal phases utilizing samples crystallized to different degrees within the calorimeter. We also estimate the number density, nucleation rate, and growth rate of the primary crystals through TEM image analysis. Our results reveal that the crystallization in this BMG has a high activation energy of ≈360 kJ/mole and that the primary crystallization of this BMG produces a high number density (≈1021 m−3 at 475 °C) of slowly growing (growth rate < 0.5 nm/s at 475 °C) Cu10(Zr,Hf)7 nanocrystals dispersed in the glassy matrix, while the second crystallization event further produces a new phase, Cu(Zr,Hf)2. The results help us to understand the GFA and thermal stability of this new BMG and provide important guidance for its future engineering applications, including its usage as a precursor to glass–crystal composite or bulk nanocrystalline structures.
Application of Ultrasound Scores (Subjective Assessment, Simple Rules Risk Assessment, ADNEX Model, O-RADS) to Adnexal Masses of Difficult Classification
Authors: Pelayo Mar , Sancho-Sauco Javier , Sánchez-Zurdo Javier , Perez-Mies Belén , Abarca-Martínez Leopoldo , Cancelo-Hidalgo Mª Jesús , Sainz-Bueno Jose Antonio , Alcázar Juan Luis , Pelayo-Delgado Irene
Published: August 2023

Background: Ultrasound features help to differentiate benign from malignant masses, and some of them are included in the ultrasound (US) scores. The main aim of this work is to describe the ultrasound features of certain adnexal masses of difficult classification and to analyse them according to the most frequently used US scores. Methods: Retrospective studies of adnexal lesions are difficult to classify by US scores in women undergoing surgery. Ultrasound characteristics were analysed, and masses were classified according to the Subjective Assessment of the ultrasonographer (SA) and other US scores (IOTA Simple Rules Risk Assessment-SRRA, ADNEX model with and without CA125 and O-RADS). Results: A total of 133 adnexal masses were studied (benign: 66.2%, n:88; malignant: 33.8%, n:45) in a sample of women with mean age 56.5 ± 7.8 years. Malignant lesions were identified by SA in all cases. Borderline ovarian tumors (n:13) were not always detected by some US scores (SRRA: 76.9%, ADNEX model without and with CA125: 76.9% and 84.6%) nor were serous carcinoma (n:19) (SRRA: 89.5%), clear cell carcinoma (n:9) (SRRA: 66.7%) or endometrioid carcinoma (n:4) (ADNEX model without CA125: 75.0%). While most teratomas and serous cystadenomas have been correctly differentiated, other benign lesions were misclassified because of the presence of solid areas or papillae. Fibromas (n:13) were better identified by SA (23.1% malignancy), but worse with the other US scores (SRRA: 69.2%, ADNEX model without and with CA125: 84.6% and 69.2%, O-RADS: 53.8%). Cystoadenofibromas (n:10) were difficult to distinguish from malignant masses via all scores except SRRA (SA: 70.0%, SRRA: 20.0%, ADNEX model without and with CA125: 60.0% and 50.0%, O-RADS: 90.0%). Mucinous cystadenomas (n:12) were misdiagnosed as malignant in more than 15% of the cases in all US scores (SA: 33.3%, SRRA: 16.7%, ADNEX model without and with CA125: 16.7% and 16.7%, O-RADS:41.7%). Brenner tumors are also difficult to classify using all scores. Conclusion: Some malignant masses (borderline ovarian tumors, serous carcinoma, clear cell carcinoma, endometrioid carcinomas) are not always detected by US scores. Fibromas, cystoadenofibromas, some mucinous cystadenomas and Brenner tumors may present solid components/papillae that may induce confusion with malignant lesions. Most teratomas and serous cystadenomas are usually correctly classified.
Predictive Role of Pretreatment Circulating miR-221 in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
Authors: Pinjaroen Nutcha , Chailapakul Piyawan , Sriphoosanaphan Supachaya , Chuaypen Natthaya , Tangkijvanich Pisit
Published: August 2023

Aberrantly expressed circulating microRNAs (miRNAs) have been demonstrated to have a crucial role in the diagnosis and prognostication of various cancers, including hepatocellular carcinoma (HCC). This research aimed to examine the role of specific miRNAs in predicting the outcomes for individuals with hepatitis B virus (HBV)-related HCC treated with transarterial chemoembolization (TACE). Stored serum specimens collected prior to the first TACE procedure were employed to determine the expression of serum miR-122, miR-221, and miR-224 using quantitative real-time PCR analysis. The study included 100 HCC patients (84% males, with an average age of 60 years) who were treated with TACE. Throughout the median follow-up spanning 18.5 months (within a range of 3 to 60 months), 42 (42.0%) patients met the criteria of TACE refractoriness. Through multivariate analysis, elevated expressed miR-221 (≥4.0 log10 copies) and advanced HCC staging were identified as independent factors related to TACE refractoriness and short overall survival. However, serum miR-122 and miR-224 levels were not linked to treatment response or overall survival. These findings underscored the potential of incorporating pretreatment levels of serum miR-221 into the established tumor staging to enhance the accurate assessment of TACE responsiveness and prognostic outcome of patients with HCC.
Ultra-High-Resolution Electrocardiography Enables Earlier Detection of Transmural and Subendocardial Myocardial Ischemia Compared to Conventional Electrocardiography
Authors: Zaichenko Kirill V. , Kordyukova Anna A. , Sonin Dmitry L. , Galagudza Michael M.
Published: August 2023

The sensitivity of exercise ECG is marginally sufficient for the detection of mild reduction of coronary blood flow in patients with early coronary atherosclerosis. Here, we describe the application of a new technique of ECG registration/analysis—ultra-high-resolution ECG (UHR ECG)—for early detection of myocardial ischemia (MIS). The utility of UHR ECG vs. conventional ECG (C ECG) was tested in anesthetized rats and pigs. Transmural MIS was induced in rats by the ligation of the left coronary artery (CA). In pigs, subendocardial ischemia of a variable extent was produced by stepwise inflation of a balloon within the right CA, causing a 25–100% reduction of its lumen. In rats, a reduction in power spectral density (PSD) in the high-frequency (HF) channel of UHR ECG was registered at 60 s after ischemia (power 0.81 ± 0.14 vs. 1.25 ± 0.12 mW at baseline, p < 0.01). This was not accompanied by any ST segment elevation on C ECG. In pigs, PSD in the HF channel of UHR ECG was significantly decreased at a 25% reduction of CA lumen, while the ST segment on C ECG remained unchanged. In conclusion, UHR ECG enabled earlier detection of transmural MIS compared to C ECG. PSD in the HF channel of UHR ECG demonstrated greater sensitivity in the settings of subendocardial ischemia.
The Differential Diagnosis of Congenital Developmental Midline Nasal Masses: Histopathological, Clinical, and Radiological Aspects
Authors: Kotowski Michal
Published: August 2023

Developmental midline nasal masses including nasal dermoids (NDs), encephaloceles (EPHCs), and nasal glial heterotopias (NGHs) are a consequence of disrupted embryonal developmental processes in the frontonasal region. Surgery is the only method of treatment in order to prevent local and intracranial inflammatory complications as well as distant deformities of the facial skeleton. Due to their rarity, similar location, and clinical and radiological symptoms, meticulous preoperative differential diagnostics is mandatory. The aim of this thorough literature review was to present and discuss all clinical, histopathological, and radiological aspects of NDs, NGHs, and EPHCs that are crucial for their differential diagnosis.
Electromagnetic Conversion into Kinetic and Thermal Energies
Authors: Brandenburg Axel , Protiti Nousaba Nasrin
Published: August 2023

The conversion of electromagnetic energy into magnetohydrodynamic energy occurs when the electric conductivity changes from negligible to finite values. This process is relevant during the epoch of reheating in the early universe at the end of inflation and before the emergence of the radiation-dominated era. We find that the conversion into kinetic and thermal energies is primarily the result of electric energy dissipation, while magnetic energy only plays a secondary role in this process. This means that since electric energy dominates over magnetic energy during inflation and reheating, significant amounts of electric energy can be converted into magnetohydrodynamic energy when conductivity emerges before the relevant length scales become stable.
A Paradigm Shift for a More Inclusive, Equal, and Just Academia? Towards a Transformative-Emancipatory Pedagogy
Authors: Cappiali Teresa Maria
Published: August 2023

This article puts forward the core argument that a pedagogical shift is very much needed if we want to address the challenges and capitalize on the opportunities represented by increasingly diverse educational settings and move towards a more inclusive, equal, and just academia. First, it is suggested that we replace the current pedagogical models in use, namely, the teacher-centered and student-centered models, as their educational philosophies are insufficient and can be considered, to a certain extent, oppressive. Then, it is argued that transformative pedagogy can broaden the scope of academic goals by including students’ well-being, as well as their individual and collective emancipatory goals. To support the argument, the author presents a theoretical framework that has been developed over time while teaching migration topics to students coming from all over the world. The framework includes a holistic approach rooted in transformative pedagogy, which engages with students’ cognitive, practical, and affective dimensions. It is further argued that the theoretical framework should integrate intersectional and decolonial approaches into its praxis. These approaches offer further insights into how to challenge power imbalances in the classroom, center the experiences and voices of marginalized communities, and recognize the interplay between individual experiences, systemic oppressions, and the broader socio-political context. The article concludes by explaining that transformative pedagogy has not yet received the attention it deserves, both in practice and in research, and that more efforts need to be made to explore its potential and scientific relevance.
Does “Out” Get You “In”? Education Outside the Classroom as a Means of Inclusion for Students with Immigrant Backgrounds
Authors: Lauterbach Gabriele , Fandrem Hildegunn , Dettweiler Ulrich
Published: August 2023

This study investigates how Education Outside the Classroom (EOtC) was used to foster the inclusion of students with immigrant backgrounds into the class. An ethnographic mixed-methods design was used, and two exemplary stories display the barriers and facilitators of inclusion in a rural school in Germany. The findings show that a lack of language proficiency and academic and social overburdening are among the main barriers to inclusion. An EOtC approach with a strong focus on place and culture responsivity, on the other hand, offers possibilities for the participation of all students and offers a promising way to more inclusive schools.
Solid-State-LiDAR-Inertial-Visual Odometry and Mapping via Quadratic Motion Model and Reflectivity Information
Authors: Yin Tao , Yao Jingzheng , Lu Yan , Na Chunrui
Published: August 2023

This paper proposes a solid-state-LiDAR-inertial-visual fusion framework containing two subsystems: the solid-state-LiDAR-inertial odometry (SSLIO) subsystem and the visual-inertial odometry (VIO) subsystem. Our SSLIO subsystem has two novelties that enable it to handle drastic acceleration and angular velocity changes: (1) the quadratic motion model is adopted in the in-frame motion compensation step of the LiDAR feature points, and (2) the system has a weight function for each residual term to ensure consistency in geometry and reflectivity. The VIO subsystem renders the global map in addition to further optimizing the state output by the SSLIO. To save computing resources, we calibrate our VIO subsystem’s extrinsic parameter indirectly in advance, instead of using real-time estimation. We test the SSLIO subsystem using publicly available datasets and a steep ramp experiment, and show that our SSLIO exhibits better performance than the state-of-the-art LiDAR-inertial SLAM algorithm Point-LIO in terms of coping with strong vibrations transmitted to the sensors due to the violent motion of the crawler robot. Furthermore, we present several outdoor field experiments evaluating our framework. The results show that our proposed multi-sensor fusion framework can achieve good robustness, localization and mapping accuracy, as well as strong real-time performance.
Neural Network Approach Super-Twisting Sliding Mode Control for Path-Tracking of Autonomous Vehicles
Authors: Kim Hakjoo , Kee Seok-Cheol
Published: August 2023

This paper proposes a neural network approach adaptive super-twisting sliding mode control algorithm for autonomous vehicles. An adaptive and robust control algorithm in autonomous vehicles is needed to compensate for disturbance and parametric uncertainty from the variable environment and vehicle conditions. The sliding mode control (SMC) is a robust controller that compensates for robust and reasonable control performance against disturbance and parametric uncertainty. However, the inherent limitation of the sliding mode control, namely the chattering phenomenon, has a negative effect on the system. Additionally, when the disturbance exceeds the defined boundaries, the control stability is compromised. To overcome these limitations, this study incorporates the radial basis function neural network (RBFNN) and Lyapunov function to estimate disturbance and parametric uncertainty. The estimated disturbance is reflected in the super-twisting sliding mode control (STSMC) to reduce the chattering phenomenon and achieve enhanced robust performance. The performance evaluation of the proposed neural network approach control algorithm is conducted using the double lane change (DLC) scenario and rapid path-tracking (RPT) scenario, implemented in the CarMaker and Matlab/Simulink environments, respectively.
A Review of Tags Anti-Collision Identification Methods Used in RFID Technology
Authors: Wang Ling , Luo Zhongqiang , Guo Ruiming , Li Yongqi
Published: August 2023

With radio frequency identification (RFID) becoming a popular wireless technology, more and more relevant applications are emerging. Therefore, anti-collision algorithms, which determine the time to tag identification and the accuracy of identification, have become very important in RFID systems. This paper presents the algorithms of ALOHA for randomness, the binary tree algorithm for determinism, and a hybrid anti-collision algorithm that combines these two algorithms. To compensate for the low throughput of traditional algorithms, RFID anti-collision algorithms based on blind source separation (BSS) are described, as the tag signals of RFID systems conform to the basic assumptions of the independent component analysis (ICA) algorithm. In the determined case, the ICA algorithm-based RFID anti-collision method is described. In the under-determined case, a combination of tag grouping with a blind separation algorithm and constrained non-negative matrix factorization (NMF) is used to separate the multi-tag mixing problem. Since the estimation of tag or frame length is the main step to solve the RFID anti-collision problem, this paper introduces an anti-collision algorithm based on machine learning to estimate the number of tags.
Untapping Industrial Flexibility via Waste Heat-Driven Pumped Thermal Energy Storage Systems
Authors: Barberis Stefano , Maccarini Simone , Shamsi Syed Safeer Mehdi , Traverso Alberto
Published: August 2023

Pumped thermal energy storage (PTES) is a promising long-duration energy storage technology. Nevertheless, PTES shows intermediate round-trip efficiency (RTE—0.5 ÷ 0.7) and significant CAPEX. sCO2 heat pumps and power cycles could reduce PTES CAPEX, particularly via reversible and flexible machines. Furthermore, the possibility to exploit freely available heat sources (such as waste heat and/or CSP inputs) could increase RTE, making the system capable of an apparent RTE > 100% as well as reducing CAPEX, avoiding the need for two TES systems. This paper analyses the potential valorization of industrial waste heat (WH) to enhance PTES thermodynamic performance as well as increase industrial energy efficiency, valorizing different levels of WH sources in the 100–400 °C temperature range. In fact, the use of additional heat, otherwise dumped into ambient surroundings, may contribute to avoiding the need for a second TES, thus enhancing plant competitiveness. Starting from an assessment of the most relevant industrial sectors to apply the proposed solution (looking at available WH and electric flexibility needed), this paper analyses the feasibility of a specific sCO2-based PTES case study, where the cycle is integrated into a cement production plant with a WH temperature of around 350 °C. It is demonstrated that the CAPEX of the proposed systems are still relevant and only a robust exploitation of the PTES in the ancillary service market could attract industrial customers’ interest in sCO2 PTES.
Investigation of Underground Coal Gasification in Laboratory Conditions: A Review of Recent Research
Authors: Kačur Ján , Laciak Marek , Durdán Milan , Flegner Patrik
Published: August 2023

The underground coal gasification (UCG) technology converts coal into product gas and provides the option of environmentally and economically attractive coal mining. Obtained syngas can be used for heating, electricity, or chemical production. Numerous laboratory coal gasification trials have been performed in the academic and industrial fields. Lab-scale tests can provide insight into the processes involved with UCG. Many tests with UCG have been performed on ex situ reactors, where different UCG techniques, the effect of gasification agents, their flow rates, pressures, and various control mechanisms to improve gasification efficiency and syngas production have been investigated. This paper provides an overview of recent research on UCG performed on a lab scale. The study focuses on UCG control variables and their optimization, the effect of gasification agents and operating pressure, and it discusses results from the gasification of various lignites and hard coals, the possibilities of steam gasification, hydrogen, and methane-oriented coal gasification, approaches in temperature modeling, changes in coal properties during gasification, and environmental risks of UCG. The review focuses on laboratory tests of UCG on ex situ reactors, results, and the possibility of knowledge transfer to in situ operation.
Mapping the Future of Green Hydrogen: Integrated Analysis of Poland and the EU’s Development Pathways to 2050
Authors: Tatarewicz Igor , Skwierz Sławomir , Lewarski Michał , Jeszke Robert , Pyrka Maciej , Sekuła Monika
Published: August 2023

This article presents the results of a comparative scenario analysis of the “green hydrogen” development pathways in Poland and the EU in the 2050 perspective. We prepared the scenarios by linking three models: two sectoral models for the power and transport sectors, and a Computable General Equilibrium model (d-Place). The basic precondition for the large-scale use of hydrogen, in both Poland and in European Union countries, is the pursuit of ambitious greenhouse gas reduction targets. The EU plans indicate that the main source of hydrogen will be renewable energy (RES). “Green hydrogen” is seen as one of the main methods with which to balance energy supply from intermittent RES, such as solar and wind. The questions that arise concern the amount of hydrogen required to meet the energy needs in Poland and Europe in decarbonized sectors of the economy, and to what extent can demand be covered by internal production. In the article, we estimated the potential of the production of “green hydrogen”, derived from electrolysis, for different scenarios of the development of the electricity sector in Poland and the EU. For 2050, it ranges from 76 to 206 PJ/y (Poland) and from 4449 to 5985 PJ/y (EU+). The role of hydrogen as an energy storage was also emphasized, highlighting its use in the process of stabilizing the electric power system. Hydrogen usage in the energy sector is projected to range from 67 to 76 PJ/y for Poland and from 1066 to 1601 PJ/y for EU+ by 2050. Depending on the scenario, this implies that between 25% and 35% of green hydrogen will be used in the power sector as a long-term energy storage.
Experimental and Numerical Evaluation of an HCCI Engine Fueled with Biogas for Power Generation under Sub-Atmospheric Conditions
Authors: Quintana Sebastián H. , Morales Rojas Andrés D. , Bedoya Iván D.
Published: August 2023

Energy transition to renewable sources and more efficient technologies is needed for sustainable development. Although this transition is expected to take a longer time in developing countries, strategies that have been widely explored by the international academic community, such as advanced combustion modes and microgeneration, could be implemented more easily. However, the implementation of these well-known strategies in developing countries requires in-depth research because of the specific technical, environmental, social, and economic conditions. The present research relies on the use of biogas-fueled HCCI engines for power generation under sub-atmospheric conditions provided by high altitudes above sea level in Colombia. A small air-cooled commercial Diesel engine was modified to run in HCCI combustion mode by controlling the air–biogas mixture temperature using an electric heater at a high speed of 1800 revolutions per minute. An experimental setup was implemented to measure and control the most important experimental variables, such as engine speed, biogas flow rate, intake temperature, crank angle degree, intake pressure, NOx emissions, and in-cylinder pressure. High intake temperature requirements of around 320 ∘C were needed to achieve stable HCCI combustion; the maximum net indicated mean effective pressure (IMEPn) was around 1.5 bar, and the highest net indicated efficiency was close to 32%. Higher intake pressures and the addition of ozone to the intake mixture were numerically studied as ways to reduce the intake temperature requirements for stable HCCI combustion and improve engine performance. These strategies were studied using a one-zone model along with detailed chemical kinetics, and the model was adjusted using the experimental results. The simulation results showed that the addition of 500 ppm of ozone could reduce the intake temperature requirements by around 50 ∘C. The experimental and numerical results achieved in this research are important for the design and implementation of HCCI engines running biogas for microgeneration systems in developing countries which exhibit more difficult conditions for HCCI combustion implementation.
Analysis of the Causes of the Emergency Shutdown of Natural Gas-Fired Water Peak Boilers at the Large Municipal Combined Heat and Power Plant
Authors: Trojan Marcin , Dzierwa Piotr , Taler Jan , Granda Mariusz , Kaczmarski Karol , Taler Dawid , Sobota Tomasz
Published: August 2023

The paper presents a cause-and-effect analysis of the failure of a 130 MWt gas-fired water boiler. The fault was a rupture of the helically finned tubes in the first rows of the second-stage water heater (ECO2). The high frequency of failures forced the boiler user to investigate their causes. The rapid drop in water flow in the ECO2 and the tightly finned pipes suggested that the permissible operating temperature of the steel used was exceeded. The only possible way to assess the working conditions was through a CFD simulation of the operation of the ECO2. Validated with the data acquisition system, the results show that the main reason for the failure was the overheating of the first rows of finned water heater pipes, regardless of the boiler load. The high heat flux value, exceeding 500,000 W/m2, and the increased flue gas temperature in front of the ECO2, almost reaching 900 °C, affected the appearance of the boiling film, limiting the cooling of the tube wall. Heat radiation and eddies behind the tubes significantly impacted the non-uniform temperature distribution, resulting in high pipe wall stress. By analyzing the service life of the first row of pipes based on the Larson–Miller parameter, it was concluded that the pipes would fail after only a few tens of hours.