bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-replication of an association between theOdd-skipped related 1c.654G>A (rs12329305) polymorphism and kidney volume in newborns
Authors: Kaczmarczyk Mariusz , Łoniewska Beata , Kuprjanowicz Anna , Boroń Agnieszka , Gorący Iwona , Ryder Małgorzata , Taryma-Leśniak Olga , Ciechanowicz Andrzej
Published: January 2020

Introduction: Odd-skipped related 1 (Osr1) protein is a marker of intermediate mesoderm from which all definitive kidney progenitor cells develop. The loss-of-functionOSR1c.654G>A (rs12329305) polymorphism has been associated with a reduced kidney volume at birth and congenital renal anomalies.

The aim of this study was to re-investigate whether theOSR1c.654A allele is associated with a smaller kidney volume (a surrogate of the congenital nephron number) in newborns, either alone or in combination withRETc.1296G>A(RETxOSR1interaction).

Materials and methods: A total of 178 healthy, full-term Polish newborns were included in this study. Kidney size was measured by ultrasound on the third day of life and normalised for body surface area (TKV/BSA). TheOSR1polymorphism was identified by mini-sequencing. The reduced model without interaction and the full model including interaction terms were compared using the F test.

Results: TKV/BSA did not differ across the genotype groups, t(176) = −0.45, p = 0.653 (106 ±24 and 108 ±25 mL/m2 for GG and GA, respectively). Similarly, there was no effect of the c.654G>A geno-type on TKV/BSA after controlling for the effect of covariates, F(1,175) = 0.28, p = 0.598. There was no association of theRETxOSR1interaction on the TKV/BSA.

Conclusion: We did not find an association between theOSR1c.1296G> A polymorphism, alone and in combination withRETc.645G> A and kidney size in a group of healthy full-term newborns.
Adenosine receptors as therapeutic targets for the treatment of myocardial infarction and its complications. Part I. Myocardial infarction
Authors: Puchałowicz Kamila , Safranow Krzysztof , Rać Monika , Chlubek Dariusz , Dziedziejko Violetta
Published: January 2020

Adenosine is an endogenous compound with cardioprotective properties that acts via A1, A2A, A2B and A3 plasma membrane receptors. Over the last several decades, the mechanisms underlying adenosine’s protective effects in the ischemic myocardium were investigated. They are the basis for ischemic preconditioning and postconditioning procedures that protect the heart from ischemia/reperfusion injury. The promising results of animal model studies have encouraged a large group of researchers to conduct clinical trials assessing the benefits of adenosine as an adjunct to reperfusion therapy in myocardial infarction. This review describes the mechanisms behind the cardioprotective effect of adenosine and presents current reports on its clinical utility in the treatment of myocardial infarction.
HPV infections in head and neck cancers (HNSCC) – clinical course and efficiency of therapy
Authors: Sikora Mateusz , Przesławska Magda , Becht Rafał
Published: January 2020

Head and neck cancers represent a heterogenous group of neoplasms. Among them, the most frequently occurring is squamous cell carcinoma. Risk factors that can contribute to the development of head and neck cancers are smoking, high proof alcohol abuse and human papilloma virus (HPV) infection. Head and neck cancers may be divided into HPV positive and HPV negative. This division shows the different landscape of carcinogen-esis, median age of onset, etiopathogenesis, clinical course and treatment efficiency. Head and neck squamous cell carcinoma (HNSCC), especially HPV positive, belongs to highly immunogenic cancers. Its development relates to HPV infection and host immune response, especially with T regulatory lymphocytes response. Programmed cell death protein-1 (PD-1), which shows immunosuppressive effect on host’s immune system, takes part in cancerogenesis. That favours carcinogenesis. The development of immuno-oncology and better understanding of HNSCC pathogenesis has contributed to developments in immunotherapy, a method of care which is used preferably in the treatment of advanced, refractory or relapsed cancers that did not respond to standard-of care cancer treatments – in the case of HNSCC it is surgical resection with possible radiochemotherapy or chemotherapy. The idea of immunotherapy is to remove tumor immunosuppressive effect and to boost host immune response to eliminate neoplasm.

This article shows a review of current knowledge and new methods of therapy concerned with HNSCC treatment, especially HPV positive HNSCC.
Reliable cell and tissue morphology-based diagnosis of endemic Burkitt lymphoma in resource-constrained settings in Ghana
Authors: Smith-Togobo Cecilia , Pedersen Mette Ø , Jensen Steffen G , Duduyemi Babatunde , Gyasi Richard K , Ofori Michael F , Paintsil Vivian , Renner Lorna , Nørgaard Peter , Hviid Lars
Published: January 2020

Endemic Burkitt lymphoma (eBL) is an aggressive B-cell lymphoma, which is a common childhood cancer in areas with intense transmission of Plasmodium falciparum parasites. Early and accurate diagnosis is a prerequisite for successful therapy, but it optimally involves advanced laboratory investigations. These are technologically demanding, expensive, and often difficult to implement in settings where eBL is prevalent. Diagnosis is thus generally based on clinical assessment and morphological examination of tumour biopsies or fine-needle aspirates (FNAs). The purpose of the present study was to assess the accuracy of eBL diagnosis at two tertiary hospitals in Ghana. To that end, we studied FNAs from 29 eBL patients and 21 non-eBL lymphoma patients originally diagnosed in 2018. In addition, we examined 111 archival formalin-fixed and paraffin-embedded (FFPE) biopsies from Ghanaian patients originally diagnosed as eBL (N=55) or non-eBL (N=56) between 2010 and 2017. Availability-based subsets of samples were subjected to haematoxylin-eosin or Giemsa staining, C-MYC immunohistochemistry, and fluorescence in situ hybridisation (FISH) analysis of c-myc rearrangements We found a good correlation between original diagnosis and subsequent retrospective assessment, particularly for FNA samples. However, evidence of intact c-myc genes and normal C-MYC expression in samples from some patients originally diagnosed as eBL indicates that morphological assessment alone can lead to eBL over-diagnosis in our study area. In addition, several FFPE samples could not be assessed retrospectively, due to poor sample quality. Therefore, the simpler FNA method of obtaining tumour material is preferable, particularly when careful processing of biopsy specimens cannot be guaranteed. We conclude that the accuracy of eBL diagnostic tools available in Ghana is generally adequate, but could be improved by implementation of additional pathology laboratory investigations. Improved attention to adequate preservation of archival samples is recommended.
The Association between Sleep Quality and Loneliness in Rural Elderly Individuals: A Cross-Sectional Study in Shandong Province, China
Authors: Jia Gaizhen , Yuan Ping
Published: January 2020

Background: There is evidence of negative associations between loneliness and sleep quality in older adults. However, little is known regarding the relationship between loneliness and sleep quality among Chinese rural elderly. This study examined the associations of loneliness and sleep quality in a cross-sectional study of older adults. Methods: A face-to-face questionnaire survey was conducted among 1,568 rural elderly people in Shandong Province, China. Loneliness was assessed using the University of California at Los Angeles Loneliness Scale. Sleep quality was assessed using the Pittsburgh Sleep Quality Index. Ordinal logistic regression was conducted to examine the association of loneliness and sleep quality after adjusting for multiple confounding variables. Results: After controlling for variables such as age, marriage, education, occupation, economic income, family relationships, living arrangement, smoking, drinking, chronic diseases, quality of life in a multivariable analysis, poor sleep quality still indicates loneliness in the rural elderly population. Conclusion: This finding implied an adverse effect of sleep quality on the loneliness of older adults. Poor sleep quality was associated with the increased odds of loneliness in Chinese rural older adults. Sleep-based interventions should be developed to prevent loneliness in rural older adults in China.
A novel five-gene signature for predicting prognosis in liver cancer
Authors: Zhong Qiuyue , Shuai Yu , Luo Qiong , Feng Guangyong , Wu Mingna , Fan Erxi , Chen Qiying , Yue Guojun , Zhang Guihai
Published: January 2020

Purpose Liver cancer is one of the most common malignant tumors in China, ranked 5th among the malignant common tumors in the world, which is still difficult to diagnose early and treat effectively. Therefore, exploring some indicators for prognostic prediction is imperative in the treatment of liver cancer. Methods Liver cancer data was obtained from The Cancer Genome Atlas (TCGA). We obtained differentially expressed genes (DEGs) by R software from TCGA database. Risk scores were acquired to assess the weighted gene-expression levels by Cox regression analysis and predict the prognosis of patients with liver cancer. Using the KEGG and GO databases, pathway enrichment was performed by identifying the analysis of DEGs. The display of receiver-operating characteristic (ROC) curves and area under the curve (AUC) could show the validity and the prognostic value of this model in liver cancer. Results In total, 1897 DEGs of transcriptome genes in liver cancer and 1197 DEGs of clinical data were extracted from the TCGA database. We identified a novel five-gene signature associated with liver cancer, including CDCA8, NR0B1, GAGE2A, AC018641.1, and SPANXC. Among of them, CDCA8 and NR0B1 were negatively related to 5-year OS, displaying a worse prognosis (P < 0.05). In particular, we also found that GAGE2A is related to lymphatic metastasis from the clinical data analysis in liver cancer. Receiver-operating characteristic (ROC) curve assessed the accuracy and sensitivity of the gene signature. In the heat map, each of the five genes for patients was presented with the distribution of the risk score. Conclusions We figured out a novel five-gene signature for the prognosis of patients with liver cancer, which may be an effective predictor for patients’ prognosis in the future.
Early veno arterial PCO2 difference is associated with outcome in peripheral veno arterial extracorporeal membrane oxygenation
Authors: Ellouze Omar , Nguyen Maxime , Missaoui Anis , Radhouani Mohamed , Berthoud Vivien , Aho Serge , Bouchot Olivier , Guinot Pierre Grégoire , Bouhemad Belaid
Published: January 2020

Background: Veno arterial membrane oxygenation (VA ECMO) is increasingly used for cardiogenic failure. However, hemodynamic targets for adequate resuscitation remain a challenge. The PCO 2 gap and the ratio between PCO 2 gap and the arteriovenous difference in oxygen (PCO 2 gap/Da–vO 2 ) are marker of peripheral hypoperfusion. We hypothesized that the PCO 2 gap and the PCO 2 gap/Da–vO 2 ratio might be useful parameters in VA ECMO patients.
Methods: We conducted an observational prospective study between September 2015 and February 2017. All consecutive patients >18 years of age who had been treated with peripheral VA ECMO for cardiac failure were included. We compared 2 groups of patients: patients who died of any cause under VA ECMO or in the 72h following VA ECMO weaning (early death group) - and patients who survived VA ECMO weaning more than 72h (surviving group). Blood samples were drawn from arterial and venous VA ECMO cannulas at H0 and H6. The ability of PCO 2 gap and PCO 2 gap/Da–vO 2 to discriminate between early mortality and surviving was studied using ROC curves analysis.
Results: We included 20 patients in surviving group and 29 in early death group. The PCO 2 gap was higher in the early death group at H6 (7.4 [5.7–10.1] vs. 5.9 [3.8–9.2], p < 0.01). AUC for PCO 2 gap at H6 was 0.76 (0.61–0.92), with a cut-off of 6.2 mmHg. The PCO 2 gap/Da–vO 2 was higher in the early death group at H0 (2.1 [1.5–2.6] vs. 1.2 [0.9–2.4], p < 0.01) and at H6 (2.1 [1.3–2.6] vs. 1.0 [0.8–1.7], p < 0.01). AUC for PCO 2 gap/Da–vO 2 at H0 and H6 were 0.79 and 0.73 respectively; the cut-off value was 1.4.
Conclusions: The PCO 2 gap and the PCO 2 gap/Da–vO2 ratio are associated with early death in patients who undergo VA ECMO.
Surveillance of the efficacy of artemisinin-piperaquine for the treatment of uncomplicated Plasmodium falciparum among children under five years in Est Mono district, Togo, 2017
Authors: Wang Qi , Lu Chenguang , Zhang Zhenyan , Yu Weisheng , Li Guoming , Pan Ziyi , Zhang Hongying , Wu Wanting , Yuan Yueming , Guo Jiawen , Liang Yuan , Huang Xinan , Guo Wenfeng , Li Changqing , Chen Meilan , Xu Qin , Song Jianping , Deng Changsheng
Published: January 2020

Background Malaria is a major public health problem in Togo. Guangzhou University of Chinese medicine of China, and the Ministry of Health and Social Security of Togo launched a nationwide artemisinin compound Mass Drug Administration Project in East Mono with a population of 150,000. Before launching the project, the sensitivity test of artemisinin piperaquine tablet was conducted in Elawagnon general clinic. On this background, we evaluated the efficacy and safety of artemisinin piperaquine in the treatment of uncomplicated falciparum malaria in children under five years of age. Methods In this study, children aged 6-59 months without complications of falciparum malaria were observed, and the selected cases were treated with artemisinin piperaquine. The patients were followed up for 28 days to observe the fever clearance time, parasitemia, gametophyte, cure rate, haemoglobin and msp-2 gene polymorphism. The primary end point was the 28-day cure rate, and PCR corrected reinfection and recrudescence. This research was conducted according to standardized WHO protocol for the assessment of the efficacy of anti-malarial treatment. Results A total of 91 children participated in the study. The adequate clinical and parasitological response (ACPR) before PCR-corrected were 66 (72.52%) and 90 (98.90%) after PCR-corrected. The patient was well tolerated to artemisinin piperaquine and no serious adverse reactions were observed. The average hemoglobin level increased by 0.05g/dl per day (p< 0.0001). The gametophyte doesn’t declined at the beginning of treatment, however, 14 days later, it dropped(D21:p<0. 05; D28: p< 0. 01). In the msp-2 gene polymorphism study of 24 children with positive parasite after treatment, 1 case of msp-2 with 3D7 haplotype and FC27 haplotype was reported, indicating that it’s recrudescence, with a frequency of 4.2% (1/24); The others maybe reinfection, with a frequency of 95.8% (23/24). Conclusion Artemisinin piperaquine was effective in treating uncomplicated falciparum malaria in children under 5 years of age in Togo and well tolerated. Plasmodium falciparum in Togo remains sensitive to artemisinin piperaquine, which could be used as a trial drug in the region. Trial Registration Trial registration: ECGPHCM No. B2017-054-01; MHSST AVIS N° 0001/2016/CBRS du 07 janvier 2016. Registered 17 March 2014, http://www.chinadrugtrials.org.cn/eap/main
Everywhere is Nowhere: A Systematic Review of Menstrual Health and Hygiene Management (MHHM) as a Human Right
Authors: Sood Suruchi , Stevens Sarah , Okumura Maho , Ramaiya Astha , Hauer Michael
Published: January 2020

Background: In many countries, stigma surrounds menstruation, which impacts several Sustainable Development Goals, including good health, quality education, gender equality, and water and sanitation. Despite its relationship with several development issues, menstrual health and hygiene management among adolescents has until recently been ignored by practitioners and researchers. This paper is a systematic review of existing literature and argues that menstrual health and hygiene management is a human rights issue, cross-cutting through development domains of health, education, nutrition, child protection, and water, sanitation, and hygiene. Methods: Four coders independently screened PubMed, Academic OneFile, and Google Scholar to obtain articles using iterations of the key terms: menstrual health; adolescents; health outcomes; education; nutrition; water, sanitation, and hygiene; and child protection. English language primary studies in peer-reviewed or grey literature articles published after 2000, that mentioned adolescents, health or hygiene, and menstruation or menstrual were included. A 9-item scale was used to rate selected full-text articles as strong, moderate, or weak. Synthesis of the results were narrative and examined the relationships between menstrual health and hygiene management and the cross-cutting domains.Results: In total, 28,745 articles were screened, with 84 articles included in the full-text review and quality assessment. Nine articles were coded as “strong” (10.8%), 46 (55.4%) as “moderate,” and 28 (33.7%) as “weak.” More than 60% of the articles examined the relationship between menstrual health and hygiene management and health (37.3%) or water, sanitation, and hygiene (25.3%). Only 11 manuscripts examined menstrual health and hygiene management as a cross-cutting issue impacting more than one development domain.Conclusions: Access to adequate facilities and menstrual health and hygiene management resources are the most common relationships documented within literature. However, there is little focus on menstrual health and hygiene management in the context of other development domains, highlighting the fact that poor menstrual health and hygiene management has not been studied as a human rights issue, negatively impacting millions of girls. Using a cross-cutting, human rights framework to address inadequate menstrual health and hygiene management is fundamental to promoting menstrual health and hygiene management with dignity among girls and women across the globe.
PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease
Authors: Thejer Bashar M. , Adhikary Partho P. , Teakel Sarah L. , Fang Johnny , Weston Paul A. , Gurusinghe Saliya , Anwer Ayad G. , Gosnell Martin , Jazayeri Jalal A. , Ludescher Marina , Gray Lesley-Ann , Pawlak Michael , Wallace Robyn H. , Pant Sameer D. , Wong Marie , Fischer Tamas , J. Elizabeth , Fehm Tanja N. , Neubauer Hans , Goldys Ewa M. , Quinn Jane C. , Weston Leslie A. , CAHILL Michael
Published: January 2020

Progesterone receptor membrane component 1 (PGRMC1) is often elevated in cancers, and exists in alternative states of phosphorylation. A motif centered on PGRMC1 Y180 was evolutionarily acquired concurrently with the embryological gastrulation organizer that orchestrates vertebrate tissue differentiation. Here, we show that mutagenic manipulation of PGRMC1 phosphorylation alters cell metabolism, genomic stability, and CpG methylation. Each of several mutants elicited distinct patterns of genomic CpG methylation. Mutation of S57A/Y180/S181A led to increased net hypermethylation, reminiscent of embryonic stem cells. Pathways enrichment analysis suggested modulation of processes related to animal cell differentiation status and tissue identity, as well as cell cycle control and ATM/ATR DNA damage repair regulation. We detected different genomic mutation rates in culture. A companion manuscript shows that these cell states dramatically affect protein abundances, cell and mitochondrial morphology, and glycolytic metabolism. We propose that PGRMC1 phosphorylation status modulates cellular plasticity mechanisms relevant to early embryological tissue differentiation.
Basic Leucine zipper (bZIP) transcription factor genes and their response to drought stress in ginseng, Panax ginseng C.A. Meyer
Authors: Li Hongjie , Chen Jing , Zhao Qi , Han Yilai , Sun Chunyu , Wang Kangyu , Wang Yanfang , Zhao Mingzhu , Chen Ping , Lei Jun , Wang Yi , Zhang Meiping
Published: January 2020

Background: As a famous and important medicinal herb in the world, ginseng contains numerous bioactive components that are remarkable for mankind's health. The basic leucine zipper (bZIP) transcription factor genes play important roles in many biological processes and plant response to abiotic and biotic stresses. Nevertheless, these genes remain unknown in ginseng. Results: Here, we report 91 bZIP genes, designated as PgbZIP genes, identified from ginseng. These PgbZIP genes were alternatively spliced into 273 transcripts. Phylogenetic analysis grouped the PgbZIP genes into ten groups, including A, B, C, D, E, F, G, H, I and S. Gene Ontology (GO) categorized the PgbZIP genes into a number of functional categories, suggesting that they have substantially diversified in functionality, even though their putative proteins share a number of conserved motifs. These 273 PgbZIP transcripts expressed quite differentially across 14 different tissues, the roots of different aged, and the roots of different cultivars. However, the expression of these transcripts was coordinated as they formed a co-expression network. Furthermore, we studied their response to drought stress in ginseng using five representatives of the PgbZIP genes, including PgbZIP25 , PgbZIP38 , PgbZIP39 , PgbZIP53 and PgbZIP54 . The results showed that these PgbZIP genes all responded to drought stress in ginseng, but the magnitudes of their response to drought stress varied. Conclusions: These results provide knowledge and resources for deeper functional analysis of PgbZIP genes and molecular tools for enhanced drought tolerance breeding in ginseng.
Histological and sex-determining genes expression effects of 17α-methyltestosterone on mandarin fish Siniperca chuatsi gonad development
Authors: Liu Shuang , Xu Peng , Liu Xuange , Guo Dingli , Chen Xiaoli , Bi Sheng , Lai Han , Zhao Xiaopin , Su Yuqing , Yi Huadong , Li Guifeng
Published: January 2020

Background 17α-methyltestosterone (MT) as a synthetic androgen has been widely used in sex reversal of fish research, but there was no published report on sex reversal of mandarin fish by MT. Moreover, comparative transcriptome analysis of testis and ovarian tissue is still lacking in mandarin fish. We used histological analyses together with RNA sequencing to characterize mandarin fish gonadal transcriptomes and investigate the effects of MT on the sex ratio, survival rate, growth, gonadal differentiation.ResultsMandarin fish treated with dietary MT at 50 mg/kg and 100 mg/kg dosages were successfully induced to all-male stock (male rate 100%), compared with the control group (51.11%). The survival rate of fish in the MT treated and control groups were not significant different. MT were significantly inhibited the growth of the MT treatment group ( P < 0.05) at the 20 dph-120 dph, however, the weight and length in the MT treated and control groups were not significant different at the 180 dph and 240 dph. MT treatment promoted the development of testis, but inhibited the gonadosomatic index (GSI) and the levels of serum steroid hormone (T and E 2 ). This work screened out the genes related to the sex determination and differentiation of the fish by sequencing and analysis of the transcriptome of the ovary and testis. The masculinization of mandarin fish was also demonstrated by the expression patterns of sex-specific genes, dmrt1, sox9, foxl2 and cyp19a1a : the gonads of MT-treated fish exclusively expressed male-specific dmrt1 and sox9 with no expression of female-specific foxl2 and cyp19a1a .Conclusion This study suggests that 17α-methyltestosterone successfully induced all-male stock and we select the part of the genes ( dmrt1, sox9, foxl2 and cyp19a1a ) related to sex determination and differentiation.
Genome-wide association study and genomic selection for plant height, maturity, seed weight, and yield in soybean
Authors: Ravelombola Waltram , Qin Jun , Shi Ainong , Wang Fengmin , Feng Yan , Meng Yaning , Yang Chunyan , Zhang Mengchen
Published: January 2020

BackgroundSoybean [Glycine max (L.) Merr.] is a legume of great interest worldwide. Enhancing genetic gain for agronomic traits via molecular approaches has been long considered as the main task for soybean breeders and geneticists. The objectives of this study were to conduct a genome-wide association study (GWAS) for these traits and identify SNP markers associated with the four traits, and to assess genomic selection (GS) accuracy.Results A total of 250 soybean accessions were evaluated for maturity, plant height, seed weight, and yield over three years. This panel was genotyped with a total of 10,259 high quality SNPs postulated from genotyping by sequencing (GBS). Population structure was inferred using STRUCTURE 2.3.4, GWAS was performed using a Bayesian Information and Linkage Disequilibrium Iteratively Nested Keyway (BLINK) model, and GS was evaluated using a ridge regression best linear unbiased predictor (rrBLUP) model. The results revealed that: a total of 20, 31, 37, 31, and 23 SNPs were significantly associated with the average 3-year data for maturity, plant height, seed weight, and yield, respectively; some significant SNPs were mapped into previously described loci (E2, E4, and Dt1) affecting maturity and plant height in soybean and a new locus mapped on chromosome 20 was significantly associated with plant height; Glyma.10g228900, Glyma.19g200800, Glyma.09g196700, and Glyma.09g038300 were candidate genes found in the vicinity of the top or the second best SNP (if no annotated genes found close the top one) for maturity, plant height, seed weight, and yield, respectively; a 11.5-Mb region of chromosome 10 was associated with both seed weight and yield; and GS accuracy was trait-, year-, and population structure-dependent.Conclusions The SNP markers identified from this study for plant height, maturity, seed weight and yield can be used to improve the four agronomic traits in soybean through marker-assisted selection (MAS) and GS in breeding programs. After validation, the candidate genes can be transferred to new cultivars using the linked SNP markers through MAS. The high GS accuracy has confirmed that the four agronomic traits can be selected in molecular breeding through GS.
MicroRNA-mRNA regulatory network related to lipid metabolism in bovine mammary epithelial cells
Authors: Xia Lixin , Zhao Zhihui , Lu Chunyan , Jiang Ping , Yu Haibin , Li Xiaohui , Yu Xiang , Liu Juan , Fang Xibi , Yang Runjun
Published: January 2020

Milk fat percentage is an important factor of milk quality in dairy cattle. Functional microRNAs and genes can affect lipid synthesis and metabolism through differential expression in bovine mammary epithelial cells (BMECs). It is necessary to screening the crucial candidate gene and miRNA on milk fat percentage. In this study, we extract total RNA of BMECs isolated from Chinese Holstein cows with high and low milk fat percentages for the conjoint analysis of RNA-seq and Solexa sequencing data. 190 differentially expressed genes and 33 differentially expressed microRNAs (DERs) were enriched in 488 GO terms and 12 KEGG pathways significantly (p <0.05) based on the conjoint analysis. The detection of triglyceride production in BMECs showed that bta-miR-21-3p and bta-miR-148a promote triglyceride synthesis, whereas bta-miR-124a, bta-miR-877, bta-miR-2382-5p and bta-miR-2425-5p inhibit triglyceride synthesis. Meanwhile, the target relationships between PDE4D and bta-miR-148a, PEG10 and bta-miR-877, SOD3 and bta-miR-2382-5p, and ADAMTS1 and bta-miR-2425-5p were verified using luciferase reporter assays and quantitative RT-PCR. The conjoint analysis can more accurately screen candidate regulator related to milk fat percentage at the molecular level, which provided a scientific research method screening functional miRNA and gene for the breeding of new high-quality dairy cows.
Inanimate figure tracing and ex vivo pig stomach models to teach endoscope control and ESD: Description of models and preliminary results.
Authors: Kumara HMC Shantha , Shah Abhinit , Pettke Erica , Sutton Elie , Bellini Geoffrey , Sandhu Jaspreet , Winkler Carl , Yan Xiaohong , Cekic Vesna , HMC Shantha Kumara , Gandhi Nipa Dilip , Whelan Richard L
Published: January 2020

Background ESD is the gold standard treatment for large sessile adenomas yet US adoption rates are low. In ESD, tissue is cut only when the scope tip, with hot knife extended, is moving; this requires different skills than needed for snare polypectomy (scope tip held still). Scope tip control and ESD training methods are needed. A program including an inanimate figure tracing model (FTM; Model1), ex vivo pig stomach model (PSM; Model2) and bovine colon model was devised. This paper concerns preliminary results for models 1 and 2.Methods A straight hollow plastic tube with a window cutout 15cm from its end was used for both models (slotted sponge = “anus”). For the FTM a paper with a line figure is placed in the window. After endoscope insertion and positioning the figure is traced with a catheter "pen"; 8 tracings (4 figure types) and 1 pig ESD were done per session. In the PSM a piece of pig stomach and Bovie pad was placed over the window. After “lifting” the mucosa ESD resection of a "polyp" was done. Time/accuracy were tracked for both models as were bowel wall injuries in the PSM.Results A total of 30 FTM sessions and PSM cases were done (2 trainees). Completion times and accuracy improved over 8 FTM sessions for less experienced trainee A but not for trainee B. A total of 29/30 PSM cases were completed. The number of deep muscle injuries decreased with time, however, case length correlated with tissue quality and not number of cases. Lifting and tissue conductivity problems were noted in poor quality tissue.Conclusions Despite model related issues, both models are feasible and allow trainees to learn scope tip control and ESD; clearly, more study is needed. A smaller diameter tube and use of fresh stomach are advised.
Removal of trihalomethanes from high organic matter water sources using aeration: a feasibility study
Authors: Mirzaei Saeideh , Gorczyca Beata
Published: January 2020

In this study, diffused aeration was applied to remove trihalomethane (THM) compounds from chlorinated, treated water containing high dissolved organic carbon (DOC) of 6.8 ± 1.2 mg/L. Increasing air-to-water volumetric ratio (rA/W) from 16 to 39 enhanced total THM (TTHM) removal from 60 to 70% at 20 °C and from 30 to 50% at 4 °C. Although bromodichloromethane has lower Henry's law constant than chloroform (CF), it was removed by a higher degree than CF in some aeration trials. Albeit obtaining high removals in aeration, TTHM reformed, and their concentration surpassed the Canadian guideline of 100 ppb in about 24 hours at 20 °C and 40 hours at 10 °C in all attempted air-to-water ratios. The water age in the system investigated in this study varied from 48 hours in midpoint chlorine boosting stations to 336 hours in the nearest endpoint. This study showed that THM removal by aeration is not a viable solution to control the concentration of these disinfection by-products in high-DOC treated water and in distribution systems where water age exceeds 24 hours; unless, it is going to be installed at the distribution endpoints.
Nalidixic Acid Schiff Bases: Synthesis and Biological Evaluation
Authors: Husain Asif , Varshney Munendra M. , Parcha Versha , Ahmad Aftab , Khan Shah A.
Published: January 2020

Background:

The prevalence of morbidity and mortality due to infections from parasitic
worms and protozoa is on rise especially in third world countries. The situation is further worsened
by drug resistant microbial pathogens.

Objectives:

The antimicrobial and anthelmintic activities associated with substituted furfuraldehyde
and 1,8-naphthyridine nucleus of nalidixic acid prompted us to synthesize some new quinolone
Schiff bases with an aim to obtain potent antibacterial and anthelmintic agents with improved safety
and efficacy.

Methods:

A new series of 1,8 naphthyridine based Schiff bases were designed and synthesized by
the reaction of Nalidixic acid methyl ester 1 with hydrazine hydrate in anhydrous condition which
yielded 1-ethyl-1,4-dihydro-7-methyl-4-oxo-1,8-naphthyridine-3-carbohydrazide 2. The compound
2 was further treated with several furfural aldehydes to furnish the desired Schiff bases (3a-k).
The in vitro antibacterial activity of Schiff bases was investigated against four Gram positive and
four Gram negative bacterial strains. The newly prepared Schiff bases were also tested for their
anthelmintic activity against Pheritima posthuma and Perionyx excavatus.

Results:

Chemical structures and identity of the prepared compounds were confirmed by their
spectral data. Overall, Schiff bases (3a-k) showed good antimicrobial activity and interestingly five
compounds exhibited more potent inhibitory effect than the standard drug Ampicillin against
S. aureus, B. cereus, E. faecalis, S. epidermidis, E. coli, S. typhi and S. dysenteriae. Schiff bases
also exhibited significant anthelmintic activity as indicated by their mean paralyses time (min) of
7.07-16.49, and 11.23- 20.46 min against Perionyx excavatus and Pheritima posthuma in comparison
to the 8.23 and 12.58 min shown by standard drug- Albendazole.

Conclusion:

It could be proposed that substitution of aromatic ring at C-5 of furfuryl heterocyclic
ring in the Schiff bases produce compounds with promising antibacterial and anthelmintic actions.
Therapeutic Approach of Probiotics in Children with Atopic Dermatitis
Authors: Huidrom Sangeeta
Published: January 2020

Pediatric atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disease, affecting
20% of children all over the world especially in developed countries. The global prevalence of
AD in children has been increasing over recent years. This chronic inflammatory skin disease causes
economic and social burden to the family. The exact cause of AD is not known, however recent studies
suggest that the imbalance of microflora present in the gut leads to AD. The current treatment of
AD involves the application of moisturizer, topical corticosteroids, antihistamines and antibiotics.
This line of treatment of AD in children has many side effects. An alternative novel therapeutic approach
has to be explored to combat this chronic skin disease. In recent years, there has been increasing
interest in the use of probiotics in the modulation of gut microbiota for the management of AD.
Many research studies showed that the administration of probiotics gives positive results in the prevention
and treatment of AD in children, however, the results are not consistent and conclusive. In
this review, the phenomenon that the dysbiosis of the gut flora contributes to the development of AD
is addressed and clinical evidence of probiotics in the prevention and treatment of AD children is also
summarised.
A Virtual Home for the Virtual Clinical Trial
Authors: Persky Susan
Published: January 2020

Virtual clinical trials (VCTs) can satisfy the need for rigorous clinical trials by using distributed technological solutions that eliminate the need for a physical trial site. This report explores potential benefits of using virtual reality (VR) to provide a “virtual site” for VCTs, a shared immersive hub in which VCT participants could experience elements of the trial and interact with the trial team. VR is a communication technology that has been emerging alongside the development of VCTs, although they have never been merged in a substantial way. Many of the gaps within the VCT paradigm are areas in which VR excels. VR environments are standardized and precisely uniform, the technology allows introduction of an almost endless set of stimuli to participants’ visual and auditory systems, and VR systems are adept at capturing precise movement and behavioral data. Although VR has not yet found its way into VCTs, much of the groundwork for such integration has been laid through research and technological development achieved in the past few years. Future implementation of VR within VCTs could move us from site-less trials to those with a virtual site serving as a hub for trial information provision, interaction with trial representatives, administration of evaluations and assessments, and more.
Machine Translation of Concise State Sentences for Quantifier Phrases
Authors: Min Wu
Published: January 2020

With the development of deep learning methods, the machine translation system based on deep neural network has reached a very high accuracy, but for some daily Chinese phenomenon machine translation system is still not able to translate correctly. In this paper, we study a sentence that often appears in Chinese spoken language, that is, a simple state sentence composed of quantitative phrases, and improve the existing machine translation system. The external helper program constructed in this paper is compatible with the current mainstream network translation systems, greatly improving the translation effect of these translation systems on the concise state sentences composed of quantitative phrases.
Language Borrowing between Arabic and Yoruba Language
Authors: Mahmoud-Mukadam Abdur-Rasheed , Adebisi Abdulwahid Aliy
Published: January 2020

Arabic Language is characterized by a great deal of influence that has made a profound impact on the rest of the world's languages, whether socially, culturally, religiously or economically. This language is specific to the Holy Quran, which has a higher constitution that Muslim takes from the laws of religion. Islam does not solve a place except it takes with language of its Arabic provisions. This article sheds light on some of the words borrowed by Yorba from Arabic in its various forms, of which there is no change in the image of pronunciation and what has undergone some change and distortion. The approach envisaged in this article is inductive, thus contributing in one way or another to supporting some scientific and historical facts in this area of borrowing. The results of this article is that language of the world is estimated relative to the world's speakers by 6.6% and the largest languages that borrow some others words in the corridors of life. Yorba, the language of southern Nigeria, and one of the three most famous tribal languages (Hausa, Yorba, Ibo) and which also has many of the speakers borrows many from Arabic.
The Values of Educational Character in the Arabic Temples of Qasidah Burdah by Imam Bushiri
Authors: Furqon M. Rizalul , Fauzi Moh. Fery
Published: January 2020

This research aimed to determine the values of educational character in the Arabic temples of Qasidah Burdah by Imam Bushiri. The method used in this research was the descriptive analytical approach, which relies on the study of reality and phenomenon as it exists in reality and was concerned as an accurate description and expressed qualitatively and quantitatively. The results of the research were that the poem of Bosiri filled with rich educational behavioral values because many people read this poem and do not know its content and meaning in detail. Behavioral educational values in the poem of Bosiri are divided into four elements: 1) Behavioral educational values divinity, 2) personality, 3) social, 4) and moral. The verses concerning the behavioral educational values in this poem eighty-two beta.
A tríade “Verdadeiro-Bom-Belo”: O lugar da beleza na Idade Média
Authors: Aertsen Jan
Published: January 2020

Neste artigo, Jan Aertsen discute a compreensão da tríade “Verdadeiro-Belo-Bom” como representante de um conjunto de questões clássicas em filosofia, a qual corresponderia, do ponto de vista moderno, à suposição de que o sujeito possui três modos distintos de se relacionar com o mundo: cognitivo, estético e prático. O autor sugere que a tríade foi formulada explicitamente pela primeira vez na Idade Média; neste período, entretanto, a beleza não era considerada um problema “estético”.
O cinema brasileiro em perspectiva: a atualidade de Brasil em Tempo de Cinema de Jean-Claude Bernadet
Authors: Bertelli Duarte André Luis
Published: January 2020

Brasil em Tempo de Cinema é a maior obra de estética cinematográfica do cinema brasileiro. Relançada quarenta anos após a edição original, mantêm-se atual, não somente devido ao rigor crítico de Bernadet sob o ponto de vista artístico, mas, sobretudo, pela pertinência das reflexões acerca dos desdobramentos culturais e políticos na história do cinema nacional.
A história invade a cena
Authors: Bertelli Duarte André Luis
Published: January 2020

A história invade, cada vez mais, as manifestações teatrais com pertinência temática e rigor metodológico. Nesse sentido, A história invade a cena é uma referência central para aqueles que estudam o modo como a cena teatral representa, em diferentes momentos, o processo histórico.
Sobre a relação entre estética e ética em Rorty e Adorno
Authors: Garcia Alves Jr. Douglas
Published: January 2020

Este artigo propõe uma análise das relações entre ética e estética nas obras de Richard Rorty e Theodor W. Adorno. Trata-se de mostrar como os dois autores abordam as origens estéticas e afetivas da moral. A hipótese proposta é a de uma primazia do prático no projeto filosófico de ambos os autores. Um breve comentário de Inteligência artificial> (EUA, 2001), de Steven Spielberg, é esboçado, para ajudar a discernir as diferenças entre as posições filosóficas discutidas, que remetem aos conceitos de “representação do sofrimento” e de “belo” (Rorty) e de “expressão do sofrimento” e de “sublime” (Adorno).
Espiritualidade mundana: O trabalho como construção do real
Authors: Camargo Sá Renata
Published: January 2020

O artigo analisa aspectos da obra de Johannes Vermeer e Richard Serra buscando esclarecer o modo pelo qual ambos baseiam sua poética na idéia de trabalho como construção do real. Tal perspectiva antecipa a substituição da noção de trabalho como punição pela de construção do mundo civilizado para a Holanda seiscentista, e aponta o artista Richard Serra como aquele que melhor representa o ponto de culminância desse processo histórico.
Brás Cubas e a solidariedade do aborrecimento humano
Authors: Cei Santos Vitor
Published: January 2020

Seguindo a máxima do personagem-narrador de que “a obra em si mesma é tudo”, o artigo visa ler o romance Memórias Póstumas de Brás Cubas a partir dos recursos oferecidos pelo próprio texto. Nesse sentido, pensamos uma questão intrínseca à narrativa, a saber: o que é a solidariedade do aborrecimento humano e qual a sua relação com galhofa e melancolia, disposições que perpassam e impulsionam toda a obra. A questão é pensada a partir de uma análise do capítulo XLII, “Que escapou a Aristóteles”, em comparação com outros trechos da obra.
A questão da criação para Nietzsche
Authors: Dias Rosa
Published: January 2020

Este artigo corresponde a comunicação proferida na mesa "Criação" do Colóquio "Gênio, criação, autoria", organizado pela Revista Viso e pela Escola de Artes Visuais do Parque Lage, e realizado nas dependências dessa instituição entre os dias 03 e 05 de novembro de 2009.
Autoria e propriedade
Authors: Ferreira Glória
Published: January 2020

Este artigo corresponde a comunicação proferida na mesa "Autoria" do Colóquio "Gênio, criação, autoria", organizado pela Revista Viso e pela Escola de Artes Visuais do Parque Lage, e realizado nas dependências dessa instituição entre os dias 03 e 05 de novembro de 2009.
O sobrevoo (des)engajado de Jacó Guinsburg
Authors: Caldas Pedro
Published: January 2020

A proposta de Rosangela Patriota e Jacó Guinsburg em "A Cena em aula" consiste em apresentar ao leitor, mediante uma abodagem ampla, as lições de Jacó Guinsburg sobre teoria e estética teatral. O livro consiste em uma coleção de documentos, tais como: notas de aula, registros de aulas, e uma entrevista com o prof. Guinsburg, que servem de testemunhos de seus cursos na Universidade de São Paulo. O livro pode ser lido como um retrato da cultura brasileira em um momento específico, mas também como uma aula de dinamismo e de teoria das idéias em crítica teatral.
Merleau-Ponty: filosofía y pintura
Authors: Larison Mariana
Published: January 2020

El presente texto intenta comprender porqué el arte y, particularmente, la pintura, se presentan, dentro de la obra del filósofo francés Maurice Merleau-Ponty, como modos ejemplares del fenómeno expresivo y nos permiten, en este sentido, comprender las características propias de la operación perceptiva.
Estética como fisiologia aplicada em Nietzsche
Authors: Nunes Bittencourt Renato
Published: January 2020

A proposta deste artigo consiste em pensarmos a relação intrínseca que Nietzsche estabelece entre a estética e a compreensão orgânica da vida humana, associada diretamente a uma perspectiva fisiológica. Cabe ressaltar que a noção de fisiologia em Nietzsche não se fundamenta em parâmetros puramente biológicos ou materiais, mas envolve todos os atos criativos do indivíduo. Por conseguinte, para compreendermos de forma precisa a realização da criatividade artística é de suma importância que se faça valer a importância da fisiologia do artista no processo de elaboração da obra de arte. Onde os instintos vitais se encontram em declínio existencial, haverá uma obra de arte decadente; onde os instintos vitais manifestam exuberância, capacidade de assimilação e saúde, haverá uma glorificação da existência através da arte.
Need for cognition and academic motivation as predictors of students’ achievement
Authors: Vasic Aleksandar
Published: January 2020

According to the self-determination theory, behind the variety of motivation,
there are just three (un)satisfied needs - for relatedness, competence and
autonomy. There are also good theoretical and empirical reasons to assume
that intrinsic motivation is, to a large extent, based on the need for
cognition. In order to test incremental validity and mediating effects of
intrinsic motivation and the need for cognition on academic achievement,
many analyses of data from a previous investigation are performed. The data
are generated applying the Scale of Academic Motivation for Students
(SAM-SI) and the shortened Scale of the Need for Cognition (NFCS-S). The
sample comprises 364 students of both gender (59% females), aged from 18 to
35 (M=20.05; SD=1.52). The predictors in hierarchical multiple regression
analyses were some sociodemographic characteristics, the need for cognition
and academic motivation, and the criteria, satisfaction with academic
achievement and aspiration. Three complete structural models are tested
through structural equation modelling. As significant predictors of
achievement and aspiration, intrinsic motivation, amotivation and the need
for cognition have been singled out. The results also indicate a significant
mediating effect of intrinsic motivation via the need for cognition on
academic achievement which contradicts the basic tenet of self-determination
theory.
Shadow Women: Wives Betrayed By Sex Buyers
Authors:   Psychologische Psychotherapeutin, Germany, Kraus Ingeborg
Published: January 2020

Shadow women are women whose husbands betray them by using prostituted women. Until now, there has been almost no attention paid to the harm to the wives or partners of men who use prostituted women. In this interview, Dr. Inge-borg Kraus talks to the former wife of a sex buyer. She describes the impact of her husband’s betrayal on her and her family. This type of harm needs to be tak-en seriously and more research done on it.