bluuebunny/crossref_metadata_2025 huggingface dataset:
University Students’ Successive Development From Entrepreneurial Intention to Behavior: The Mediating Role of Commitment and Moderating Role of Family Support
Authors: Mei Hu , Ma Zicheng , Zhan Zehui , Ning Wantong , Zuo Huiqi , Wang Jinbin , Huang Yingying
Published: March 2022

University students having high entrepreneurial intention while not transferring into actual entrepreneurial behavior is a contradictory issue in need of in-depth research. To explore the successive development mechanism of the entrepreneurial process, this study constructed a moderated mediation model to examine whether entrepreneurial commitment from three dimensions (affective, behavioral, and continuance) mediated the relationship between entrepreneurial intention and behavior, and whether this mediating process was moderated by family support. A survey was conducted among university students from six major universities in south China using the snowball sampling approach. A total of 469 valid responses were obtained (44.6% male and 55.4% female participants). Structural equation modeling was adopted for data analysis. According to the results of the confirmatory factor analysis, it was found that entrepreneurial intention had both direct and indirect positive effects on entrepreneurial behavior, while entrepreneurial commitment worked as the mediator, and family support moderated the relationship between entrepreneurial intention and behavior. Results indicated that entrepreneurial commitment bridged the path from entrepreneurial intention to behavior, and family support created the boundary effect. This finding highlights the importance of guiding students through entrepreneurial commitment toward entrepreneurial behavior, and pays special attention to the crucial role of family support under the national strategy.
What Is Targeted When We Train Working Memory? Evidence From a Meta-Analysis of the Neural Correlates of Working Memory Training Using Activation Likelihood Estimation
Authors: Vartanian Oshin , Replete Vladyslava , Saint Sidney Ann , Lam Quan , Forbes Sarah , Beaudoin Monique E. , Brunyé Tad T. , Bryant David J. , Feltman Kathryn A. , Heaton Kristin J. , McKinley Richard A. , Van Erp Jan B. F. , Vergin Annika , Whittaker Annalise
Published: March 2022

Working memory (WM) is the system responsible for maintaining and manipulating information, in the face of ongoing distraction. In turn, WM span is perceived to be an individual-differences construct reflecting the limited capacity of this system. Recently, however, there has been some evidence to suggest that WM capacity can increase through training, raising the possibility that training can functionally alter the neural structures supporting WM. To address the hypothesis that the neural substrates underlying WM are targeted by training, we conducted a meta-analysis of functional magnetic resonance imaging (fMRI) studies of WM training using Activation Likelihood Estimation (ALE). Our results demonstrate that WM training is associated exclusively with decreases in blood oxygenation level-dependent (BOLD) responses in clusters within the fronto-parietal system that underlie WM, including the bilateral inferior parietal lobule (BA 39/40), middle (BA 9) and superior (BA 6) frontal gyri, and medial frontal gyrus bordering on the cingulate gyrus (BA 8/32). We discuss the various psychological and physiological mechanisms that could be responsible for the observed reductions in the BOLD signal in relation to WM training, and consider their implications for the construct of WM span as a limited resource.
Predictors of Treatment Response to an Internet-Delivered Intervention Targeting Residual Cognitive Symptoms After Major Depressive Disorder
Authors: Myklebost Sunniva Brurok , Gjestad Rolf , Inal Yavuz , Hammar Åsa , Nordgreen Tine
Published: March 2022

Objective

Residual cognitive symptoms after depression are common and associated with reduced daily life functioning and an increased risk of depression relapse. There is a lack of knowledge on treatments targeting residual cognitive symptoms after major depressive disorder (MDD), including the factors associated with treatment response. The aim of the current study is to explore factors of treatment response to a guided internet-delivered intervention for former depressed adults experiencing residual cognitive symptoms.

Method

Forty-three former depressed adults with residual cognitive symptoms were included. Linear mixed model analyses were used to investigate the impact of pre-treatment demographic-, illness, and symptom variables, and therapy process variables, such as credibility, expectancy, and user behavior, on reduction in residual cognitive symptoms from pre-treatment to 6-month follow-up.

Results

Having had MDD for a year or less predicted more reductions in residual cognitive symptoms from pre- to 6-month follow-up. Higher levels of perceived treatment credibility and expectancy evaluated in the early course of treatment did also predict a positive treatment response. No demographic-, symptom-variables, previous number of episodes with MDD, and user behavior were associated with change in residual cognitive symptoms.

Conclusion

This study suggests that individuals with shorter duration of previous depressions might have larger reductions in residual cognitive symptoms at 6-month follow-up compared to those with a longer duration of depression. Treatment credibility and expectancy also predicted treatment response and effort should also be made to ensure interventions credibility. Results should be interpreted with caution due to the study having a low sample size. Further investigation of predictors should be conducted in a full scale randomized controlled trial.
Connections and Biases in Health Equity and Culture Research: A Semantic Network Analysis
Authors: Martínez-García Mireya , Villegas Camacho José Manuel , Hernández-Lemus Enrique
Published: March 2022

Health equity is a rather complex issue. Social context and economical disparities, are known to be determining factors. Cultural and educational constrains however, are also important contributors to the establishment and development of health inequities. As an important starting point for a comprehensive discussion, a detailed analysis of the literature corpus is thus desirable: we need to recognize what has been done, under what circumstances, even what possible sources of bias exist in our current discussion on this relevant issue. By finding these trends and biases we will be better equipped to modulate them and find avenues that may lead us to a more integrated view of health inequity, potentially enhancing our capabilities to intervene to ameliorate it. In this study, we characterized at a large scale, the social and cultural determinants most frequently reported in current global research of health inequity and the interrelationships among them in different populations under diverse contexts. We used a data/literature mining approach to the current literature followed by a semantic network analysis of the interrelationships discovered. The analyzed structured corpus consisted in circa 950 articles categorized by means of the Medical Subheadings (MeSH) content-descriptor from 2014 to 2021. Further analyses involved systematic searches in the LILACS and DOAJ databases, as additional sources. The use of data analytics techniques allowed us to find a number of non-trivial connections, pointed out to existing biases and under-represented issues and let us discuss what are the most relevant concepts that are (and are not) being discussed in the context of Health Equity and Culture.
Adolescent Sexual and Reproductive Health During the COVID-19 Pandemic: A Mini Review
Authors: Groenewald Candice , Isaacs Nazeema , Isaacs Dane
Published: March 2022

This mini review explores the impact of the COVID-19 pandemic on adolescent sexual and reproductive health. We conducted a rapid review of the literature across three databases, with a particular focus on the African continent. Few studies have specifically focused on adolescents in Africa and this paper contributes to this paucity of research. Findings revealed the unintended consequences of the pandemic. Studies across several countries showed that the respective lockdown measures restricted adolescents' access to sexual and reproductive health services. The literature also showed increases in adolescent pregnancies during the lockdown, along with increases in reports of sexual violence against adolescents. We conclude this paper by offering recommendations to address these unintended consequences and potentially improve adolescent sexual and reproductive health in African communities.
Can Kids Be Allergic to the Sun?
Authors: Raef Haya S. , Richmond Jillian M.
Published: March 2022

Some people may experience an unusual and dramatic skin response to light exposure. This is called photosensitivity. Patients with photosensitivity may develop a rash, or symptoms such as swelling, itch, numbness, or pain after exposure to light. Symptoms usually follow exposure to sunlight, but artificial light sources such as light bulbs may also cause skin reactions. While photosensitivity is more common in adults, it also affects children, and certain photosensitivity conditions can begin in childhood. In this article, we discuss ways that kids can be sensitive to sunlight and the negative impact this can have on the mental health of affected individuals.
Counting Animals By Recording Their Voices
Authors: Marques Tiago A. , Dornellas Luisa F. , Guerra Anaís , Marques Carolina S. , Tempero Beatriz , Zacarias Maria , Hart Caryl
Published: March 2022

Across the world, our wildlife is under threat. Global warming, farming, fishing, buildings, and roads are eating up or damaging wild spaces where animals live. Animals like the vaquita, the world’s rarest marine mammal, are on the brink of extinction. To look after the wildlife that we still have, conservationists need to know how many animals there are, and how populations are changing over time. But how do you work out how many gibbons are left in the rainforest, or how many porpoises there are in the sea, if you cannot see them? We work out population sizes by recording and counting the calls or songs animals make. We then use this information to help conservationists make better decisions about how to look after some of our rarest and most endangered species, now and into the future.
Advancing Food System Transformation and Addressing Conflicts Through Transdisciplinary Methodologies: Strengths and Limitations of the Community Voice Method, T-Labs, Film-Making and the Miracle Question
Authors: Calla Simon , Lécuyer Lou , Skrimizea Eirini , Balian Estelle , Young Juliette C.
Published: March 2022

Food systems are changing through various socioeconomic and policy processes. For example, in France, following concerns over the effects of pesticides on ecosystems and health, the French government launched the “Ecophyto II+” plan in 2019 that aims for a 50% reduction in the use of pesticides by 2025. This top-down food system transformation is leading to conflicts between stakeholders over how to enact such a policy, and its implications for farmers and their practices. By adopting a transdisciplinary research approach, we explore conflicts linked to food system transformations in the context of three case studies in France. The case studies revolve around conflicts over pesticide use and reduction in three agricultural settings in Bourgogne Franche-Comté, namely (a) water management near Auxerre, (b) apiculture-agriculture relations in the Jura, and (c) viticulture-local resident relationships near Macon. We use four innovative transdisciplinary techniques to integrate inclusively the viewpoints of diverse stakeholders with the aim of generating actionable responses to transform food systems. First, the Community Voice Method (CVM) includes filmed semi-structured interviews and integrates a number of opportunities for participation and successive rounds of data analysis. Second, the interviewees were asked a “miracle question” that encouraged them to step back from conflicts and practices toward their ideal vision of agriculture and food systems. Third, the CVM resulted in the production of four films that relate the visions and perception of each case study interviewees in their own words and in their own setting. Finally, Transformation Labs (T-Labs) conveyed the main results of the CVM knowledge synthesis through the films produced and opened a dialogue toward the development of solutions. We review the four techniques, how they were implemented in the three case studies, and with which outcomes. Thus the aim of this paper is to offer reflections and lessons learnt from different transdisciplinary processes as a means of strengthening their application in other contexts. We argue that such methodologies, whilst resource-consuming, are essential to fully understand the complexity of food system transformations from the often-conflictual perspectives and competing knowledge claims of the multiple actors involved. In addition, we highlight the role of these techniques in building long-term trust between researchers and other stakeholders, and the benefits in terms of opening up dialogue and developing long-term solutions, as determined by the stakeholders themselves.
Can Digitalization Levels Affect Agricultural Total Factor Productivity? Evidence From China
Authors: Fu Wenqiang , Zhang Rongwu
Published: March 2022

The agricultural sector faces a food crisis and major challenges in green and sustainable development, and digital technology is an important countermeasure. In this paper, “digital technology” is used as a keyword to construct a regional digitalization level indicator. China's provincial panel data from 2013 to 2020 are adopted as samples to explore the impact of regional digitalization levels on agricultural total factor productivity and its mechanism. The study found the following: (1) Regional digitalization can significantly raise agricultural total factor productivity. (2) Regional digitalization can significantly raise agricultural total factor productivity in economically underdeveloped areas but not in economically developed areas. (3) The mitigation of factor market distortion and large-scale production can strengthen the role of regional digitalization in raising agricultural total factor productivity.
From Qualitative to Quantitative AOP: A Case Study of Neurodegeneration
Authors: Sinitsyn Dennis , Garcia-Reyero Natàlia , Watanabe Karen H.
Published: March 2022

Adverse outcome pathways (AOPs) include a sequence of events that connect a molecular-level initiating event with an adverse outcome at the cellular level for human health endpoints, or at the population level for ecological endpoints. When there is enough quantitative understanding of the relationships between key events in an AOP, a mathematical model may be developed to connect key events in a quantitative AOP (qAOP). Ideally, a qAOP will reduce the time and resources spent for chemical toxicity testing and risk assessment and enable the extrapolation of data collected at the molecular-level by in vitro assays, for example, to predict whether an adverse outcome may occur. Here, we review AOPs in the AOPWiki, an AOP repository, to determine best practices that would facilitate conversion from AOP to qAOP. Then, focusing on a particular case study, acetylcholinesterase inhibition leading to neurodegeneration, we describe specific methods and challenges. Examples of challenges include the availability and collection of quantitative data amenable to model development, the lack of studies that measure multiple key events, and model accessibility or transferability across platforms. We conclude with recommendations for improving key event and key event relationship descriptions in the AOPWiki that facilitate the transition of qualitative AOPs to qAOPs.
Analysis of Antimicrobial Resistance in Non-typhoidal Salmonella Collected From Pork Retail Outlets and Slaughterhouses in Vietnam Using Whole Genome Sequencing
Authors: Holohan Niamh , Wallat Maximilian , Hai Yen Luu Thi , Clark Eleanor , Truong Duong Thi Quy , Xuan Sinh Dang , Vu Hue Thi Kim , Van Truong Dung , Tran Huy Hoang , Nguyen-Viet Hung , Unger Fred , Thi Thanh Dang Son , Stabler Richard A.
Published: March 2022

Non-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergence of colistin resistance, a “last defense” antibioic against multi-drug resistant (MDR) Gram-negative pathogens. This study aimed to investigate the antibiotic susceptibility of 69 NTS isolates collected from the pork retail outlets and slaughterhouses in Vietnam during 2014 a nd 2018/19. Phenotypic testing and whole genome sequencing was used to assess the serotype and AMR gene profiles of the 69 NTS isolates. Seventeen different serotypes were identified, of which S. enterica subsp enterica serotype Typhimurium was the most common followed by S. ser. Rissen, S. ser. London, S. ser. Anatum, and S. ser. Derby. Phenotype AMR was common with 41 (59.4%) isolates deemed MDR. MDR strains were most common in slaughterhouses (83%) and supermarkets (75%) and lowest in traditional markets (38%) and convenience stores (40%). Colistin resistance was identified in 18 strains (15 resistant, three intermediate) with mcr-1 identified in seven isolates (S. ser. Meleagridis, S. Rissen, S. Derby) and mcr-3 in two isolates (S. Typhimurium). This includes the first mcr positive S. Meleagridis to our knowledge. Surprisingly, boutique stores had high levels (60%) of MDR isolates including 5/20 isolates with mcr-1. This study demonstrates that pork from modern retail stores classed as supermarkets or boutique (with pork claiming to be high quality, traceable, environmentally friendly marketed toward higher income consumers) still contained NTS with high levels of AMR.
The Antimicrobial Peptide MPX Can Kill Staphylococcus aureus, Reduce Biofilm Formation, and Effectively Treat Bacterial Skin Infections in Mice
Authors: Zhu Chunling , Zhao Yaya , Zhao Xueqin , Liu Shanqin , Xia Xiaojing , Zhang Shouping , Wang Yimin , Zhang Huihui , Xu Yanzhao , Chen Shijun , Jiang Jinqing , Wu Yundi , Wu Xilong , Zhang Gaiping , Bai Yueyu , Hu Jianhe , Fotina Hanna , Wang Lei , Zhang Xueming
Published: March 2022

Staphylococcus aureus is a common pathogen that can cause pneumonia and a variety of skin diseases. Skin injuries have a high risk of colonization by S. aureus, which increases morbidity and mortality. Due to the emergence of multidrug-resistant strains, antimicrobial peptides are considered to be among the best alternatives to antibiotics due to their unique mechanism of action and other characteristics. MPX is an antibacterial peptide extracted from wasp venom that has antibacterial activity against a variety of bacteria. This study revealed that MPX has good bactericidal activity against S. aureus and that its minimum inhibitory concentration (MIC) is 0.08 μM. MPX (4×MIC) can kill 99.9% of bacteria within 1 h, and MPX has good stability. The research on the bactericidal mechanism found that MPX could destroy the membrane integrity, increase the membrane permeability, change the membrane electromotive force, and cause cellular content leakage, resulting in bactericidal activity. Results from a mouse scratch model experiment results show that MPX can inhibit colonization by S. aureus, which reduces the wound size, decreases inflammation, and promotes wound healing. This study reports the activity of MPX against S. aureus and its mechanism and reveals the ability of MPX to treat S. aureus infection in mice, laying the foundation for the development of new drugs for bacterial infections.
Efficacy of Tricaine (MS-222) and Hypothermia as Anesthetic Agents for Blocking Sensorimotor Responses in Larval Zebrafish
Authors: Leyden Claire , Brüggemann Timo , Debinski Florentyna , Simacek Clara A. , Dehmelt Florian A. , Arrenberg Aristides B.
Published: March 2022

Tricaine, or MS-222, is the most commonly used chemical anesthetic in zebrafish research. It is thought to act via blocking voltage-gated sodium channels, though its mechanism of action, particularly at the neuronal level, is not yet fully understood. Here, we first characterized the effects of tricaine on both body balance and touch responses in freely swimming animals, before determining its effect on the neural activity underlying the optokinetic response at the level of motion perception, sensorimotor signaling and the generation of behavior in immobilized animals. We found that the standard dose for larvae (168 mg/L) induced loss of righting reflex within 30 seconds, which then recovered within 3 minutes. Optokinetic behavior recovered within 15 minutes. Calcium imaging showed that tricaine interferes with optokinetic behavior by interruption of the signals between the pretectum and hindbrain. The motion sensitivity indices of identified sensory neurons were unchanged in larvae exposed to tricaine, though fewer such neurons were detected, leaving a small population of active sensory neurons. We then compared tricaine with gradual cooling, a potential non-chemical alternative method of anesthesia. While neuronal tuning appeared to be affected in a similar manner during gradual cooling, gradual cooling induced a surge in calcium levels in both the pretectum and hindbrain. This calcium surge, alongside a drop in heartrate, is potentially associated with harmful changes in physiology and suggests that tricaine is a better anesthetic agent than gradual cooling for zebrafish laboratory research.
Municipal Rating System—A Municipality Compliance Index
Authors: Meirinhos Galvão , Bessa Maximino , Leal Carmem , Silva Rui
Published: March 2022

This research paper presents and discusses the main results generated and obtained with the proprietary computer platform CIDIUS®, developed by the authors of this work, which aims to support the decision-making process of Portuguese mayors. Thus, keeping in mind the theoretical models and based on the data collected through the questionnaire given to the population, we tried to understand the influence that the dimensions Notoriety, Image, and Reputation (NIR), Citizen and Voter Expectations (CVE), Contestation and Complaint of the Municipal Executive (CCME), Perceived Value (PV), and Organizational Performance and Perceived Quality (OPPQ) has a positive effect on Municipe Satisfaction (MS). The parishes of the municipality of Valongo were selected and analyzed, namely the parishes of Alfena, Campo e Sobrado, Valongo, and Ermesinde, and a total of 998 valid questionnaires were collected. It was concluded that all studied dimensions except the Organizational Performance and Perceived Quality (OPPQ) dimension had a positive and statistically significant impact on Municipe Satisfaction (MS). The results of this research suggest the need for the use of these opinion-gathering techniques to encourage active citizen involvement in the daily life of their municipality, as well as the need for valid information that gives executives the ability to take political action that is appropriate to the interests and expectations of citizens.
A Two-Year Field Experiment for the Integrated Management of Bread and Durum Wheat Fungal Diseases and of Deoxynivalenol Accumulation in the Grain in Central Italy
Authors: Balducci Emilio , Tini Francesco , Beccari Giovanni , Ricci Giacomo , Ceron-Bustamante Minely , Orfei Maurizio , Guiducci Marcello , Covarelli Lorenzo
Published: March 2022

A two-year (2018/19 and 2019/20) field experiment was carried out to evaluate the efficacy of recently developed fungicide combinations (with different modes of action) towards fungal diseases on seven bread and eight durum wheat varieties. The trial was performed at the FIELDLAB experimental station of the University of Perugia (Italy). The diseases were assessed under natural pressure except for Fusarium head blight (FHB) for which artificial inoculation with a Fusarium culmorum deoxynivalenol (DON)-producing strain was performed at the full flowering stage (BBCH 65). Fungicides were sprayed at the fully extended flag leaf (BBCH 39) and full flowering (BBCH 65) stages. The incidence of different fungal diseases was visually evaluated and other parameters [grains production (t/ha), protein content (%), test weight (kg/hL), and DON accumulation in grain (μg/kg)] were also determined. In the two years, characterized by different climatic conditions, the fungicide treatments showed efficacy in controlling the observed diseases (Septoria tritici blotch and FHB) as well as in reducing DON contamination. No significant differences were found between treatments. The results highlight that, in the present scenario of commercially available durum and bread wheat varieties, the timely application of the most common fungicides plays a crucial role for FHB and DON management in the presence of climatic conditions that are favorable to the disease. The impact of these results in an integrated disease management perspective is discussed.
Effect of Dexamethasone and Route of Administration on Sow Farrowing Behaviours, Piglet Delivery and Litter Performance
Authors: Ward Sophia A. , Kirkwood Roy N. , Song Yunmei , Garg Sanjay , Plush Kate J.
Published: March 2022

The inflammatory pain and stress some crated sows experience during farrowing has attendant risks of piglet-directed aggression, reduced teat exposure and hindered post-partum recovery. To counter this, the steroidal anti-inflammatory compound, dexamethasone, can be administered. To measure the potential for mucosal absorption as an alternative to injection, the permeability of porcine vaginal mucosa to dexamethasone was demonstrated using Franz cell diffusion. These studies found dexamethasone treatment diffused through vaginal mucosa at a constant rate, with 52.37 ± 5.54% permeation in 6 h. To examine in vivo effects on farrowing outcomes, dexamethasone was administered to gilts and parity one sows on the day of expected farrowing. We hypothesized that it would provide relief from farrowing discomfort and reduce behaviours threatening piglet survival. Sows were randomly assigned to receive dexamethasone as an intramuscular injection (n = 23); dexamethasone applied topically into the vagina (n = 20), or to receive no dexamethasone (n = 23). Sows (n = 66) and piglets (n = 593) were monitored for performance indicators during farrowing and early lactation. A subset of sows (n = 24) was also video monitored continuously over 24 h for behaviours associated with pain, postural changes and piglet interactions. No differences were observed between treatment for farrowing performance, piglet survival or behavioural changes for sows experiencing their first or second farrowing (p > 0.05), rejecting the hypothesis that corticosteroid administration will improve sow farrowing performance. This investigation did, however, show that dexamethasone can permeate through porcine vaginal mucosa and so can be administered as a non-injectable treatment.
Trauma in Animal Protection and Welfare Work: The Potential of Trauma-Informed Practice
Authors: Stevenson Rochelle , Morales Celeste
Published: March 2022

Those who work in the animal protection and welfare (APW) sector are consistently exposed to human and animal suffering, particularly those who witness animal surrenders and seizures. Continued exposure to suffering can result in stress, anxiety, burnout, and compassion fatigue, which are detrimental to individual and organizational well-being. The aim of this study was to understand the challenges experienced by Canadian APW workers, and to explore how trauma-informed approaches can be implemented to help mitigate these challenges. To achieve this, we utilized purposive sampling to seek workers in the APW sector who had experience with animal surrender and/or seizure. Telephone interviews were conducted with 11 participants. Participants reported experiencing many challenges that negatively impacted their mental health; this article summarizes them by focusing on two key themes drawn from the narratives of the participants: feeling unprepared and forced strength. Trauma-informed practices are explored as a means to prevent compassion fatigue and burnout, and to increase the resilience of individuals and organizations. We suggest trauma-informed practices help APW workers manage job-related stressors while also providing a more compassionate experience for animal guardians. Further, we propose that trauma-informed practices are a crucial component in facilitating respectful relationships with the communities that APW organizations serve.
Use of Fertility Control (Nicarbazin) in Barcelona: An Effective yet Respectful Method towards Animal Welfare for the Management of Conflictive Feral Pigeon Colonies
Authors: González-Crespo Carlos , Lavín Santiago
Published: March 2022

This study describes a three-year evaluation (2017–2019) of a fertility control protocol using nicarbazin (Ovistop®) to reduce the abundance of the most conflictive colonies of feral pigeon, Columba livia var. domestica, in Barcelona, Spain, as a long-term strategy based on animal welfare. The treatment was supplied to 34 pigeon colonies by automatic hopper feeders installed in public areas. A superiority study and a population monitoring study were carried out to evaluate differences in the abundance of the colonies, as well as the proportion of juveniles, the possible intake of nicarbazin by non-target species and the movement of individuals among colonies. The results showed statistical differences in the population trends between the test (−22.03%) and control (+12.86%) groups, and a significant steady decreasing trend in the pigeon abundance (−55.26%) was registered until the end of 2019. The proportion of juveniles was significatively lower in the test colonies, and a non-target species (Eurasian collared doves, Streptopelia decaocto) was observed consuming in a residual form. The protocol using nicarbazin is able to both control the abundance of pigeons, with no impact over non-target species, and respond to the public interest about animal welfare by providing an ethical method to manage overabundant and/or conflictive populations.
Effects of Common Litter Management Practices on the Prevalence of Campylobacter jejuni in Broilers
Authors: Bailey Matthew A. , Bourassa Dianna V. , Krehling James T. , Munoz Luis , Chasteen Kaicie S. , Escobar Cesar , Macklin Kenneth S.
Published: March 2022

Campylobacter is an important foodborne pathogen and is naturally found in chickens. During broiler production, litter can become contaminated with Campylobacter when birds defecate, and this litter, in some countries, is typically reused for the next flock, potentially causing cross-contamination. The goal of this experiment was to observe if reusing contaminated litter could spread Campylobacter between flocks and to observe if common litter treatments could prevent this cross-contamination. To determine this, a flock of birds was inoculated with Campylobacter jejuni and allowed to naturally contaminate the litter for 42 days. After grow-out, birds were terminated, and litter was given five treatments: uninoculated fresh litter, untreated re-used litter, composted re-used litter, re-used litter treated with sodium bisulfate (45 kg/305 m2), and re-used litter composted and treated with sodium bisulfate (45 kg/305 m2). A second flock was placed on the litter, grown for 42 days, and tested for C. jejuni prevalence. Following inoculation of the first flock, high prevalence of C. jejuni was observed; however, after a 19-day down-time between flocks, no C. jejuni was detected in any samples from the second flock. These results indicate that re-used litter was not a significant reservoir for cross-contamination of broilers when provided a significant down-time between flocks.
Phase-In to Phase-Out—Targeted, Inclusive Strategies Are Needed to Enable Full Replacement of Animal Use in the European Union
Authors: Marshall Lindsay J. , Constantino Helder , Seidle Troy
Published: March 2022

In September 2021, the European Parliament voted overwhelmingly in favour of a resolution to phase out animal use for research, testing, and education, through the adoption of an action plan. Here we explore the opportunity that the action plan could offer in developing a more holistic outlook for fundamental and biomedical research, which accounts for around 70% of all animal use for scientific purposes in the EU. We specifically focus on biomedical research to consider how mapping scientific advances to patient needs, taking into account the ambitious health policies of the EU, would facilitate the development of non-animal strategies to deliver safe and effective medicines, for example. We consider what is needed to help accelerate the move away from animal use, taking account of all stakeholders and setting ambitious but realistic targets for the total replacement of animals. Importantly, we envisage this as a ‘phase-in’ approach, encouraging the use of human-relevant NAMs, enabling their development and application across research (with applications for toxicology testing). We make recommendations for three pillars of activity, inspired by similar efforts for making the shift to renewable energy and reducing carbon emissions, and point out where investment—both financial and personnel—may be needed.
Menthae Herba Attenuates Neuroinflammation by Regulating CREB/Nrf2/HO-1 Pathway in BV2 Microglial Cells
Authors: Park Yeo Jin , Yang Hye Jin , Li Wei , Oh You-Chang , Go Younghoon
Published: March 2022

Chronic inflammation and oxidative stress cause microglia to be abnormally activated in the brain, resulting in neurodegenerative diseases such as Alzheimer’s disease (AD). Menthae Herba (MH) has been widely used as a medicinal plant with antimicrobial, anti-inflammatory, and antioxidant properties. In this study, we sought to evaluate the effects of MH on the inflammatory response and possible molecular mechanisms in microglia stimulated with lipopolysaccharide (LPS). Transcriptional and translational expression levels of the proinflammatory factors were measured using ELISA, RT-qPCR, and Western blot analysis. MH extract inhibited the production of proinflammatory enzymes and mediators nitric oxide (NO), NO synthase, cyclooxygenase-2, tumor necrosis factor-α, and interleukin-6 in LPS-stimulated cells. Our molecular mechanism study showed that MH inhibited the production of reactive oxygen species (ROS) and the phosphorylation of mitogen-activated protein kinase and nuclear factor (NF)-κB. In contrast, MH activated HO-1 and its transcriptional factors, cAMP response element-binding protein (CREB), and the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways. Thus, MH reduces ROS and NF-κB-mediated inflammatory signaling and induces CREB/Nrf2/HO-1-related antioxidant signaling in microglia. Together, these results may provide specific prospects for the therapeutic use of MH in the context of neuroinflammatory diseases, including AD.
Angiotensin II Promotes Skeletal Muscle Angiogenesis Induced by Volume-Dependent Aerobic Exercise Training: Effects on miRNAs-27a/b and Oxidant–Antioxidant Balance
Authors: Rodrigues Luis Felipe , Pelozin Bruno Rocha Avila , da Silva Junior Natan Daniel , Soci Ursula Paula Renó , do Carmo Everton Crivoi , da Mota Glória de Fatima Alves , Cachofeiro Victoria , Lahera Vicente , Oliveira Edilamar Menezes , Fernandes Tiago
Published: March 2022

Aerobic exercise training (ET) produces beneficial adaptations in skeletal muscles, including angiogenesis. The renin–angiotensin system (RAS) is highly involved in angiogenesis stimuli. However, the molecular mechanisms underlying capillary growth in skeletal muscle induced by aerobic ET are not completely understood. This study aimed to investigate the effects of volume-dependent aerobic ET on skeletal muscle angiogenesis involving the expression of miRNAs-27a and 27b on RAS and oxidant–antioxidant balance. Eight-week-old female Wistar rats were divided into three groups: sedentary control (SC), trained protocol 1 (P1), and trained protocol 2 (P2). P1 consisted of 60 min/day of swimming, 5×/week, for 10 weeks. P2 consisted of the same protocol as P1 until the 8th week, but in the 9th week, rats trained 2×/day, and in the 10th week, trained 3×/day. Angiogenesis and molecular analyses were performed in soleus muscle samples. Furthermore, to establish ET-induced angiogenesis through RAS, animals were treated with an AT1 receptor blocker (losartan). Aerobic ET promoted higher VO2 peak and exercise tolerance values. In contrast, miRNA-27a and -27b levels were reduced in both trained groups, compared with the SC group. This was in parallel with an increase in the ACE1/Ang II/VEGF axis, which led to a higher capillary-to-fiber ratio. Moreover, aerobic ET induced an antioxidant profile increasing skeletal muscle SOD2 and catalase gene expression, which was accompanied by high nitrite levels and reduced nitrotyrosine concentrations in the circulation. Additionally, losartan treatment partially re-established the miRNAs expression and the capillary-to-fiber ratio in the trained groups. In summary, aerobic ET promoted angiogenesis through the miRNA-27a/b–ACE1/Ang II/VEGF axis and improved the redox balance. Losartan treatment demonstrates the participation of RAS in ET-induced vascular growth. miRNAs and RAS components are promising potential targets to modulate angiogenesis for combating vascular diseases, as well as potential biomarkers to monitor training interventions and physical performance.
Erythropoietin as a Neuroprotective Drug for Newborn Infants: Ten Years after the First Use
Authors: Perrone Serafina , Lembo Chiara , Gironi Federica , Petrolini Chiara , Catalucci Tiziana , Corbo Giulia , Buonocore Giuseppe , Gitto Eloisa , Esposito Susanna Maria Roberta
Published: March 2022

Protective strategies against perinatal brain injury represent a major challenge for modern neonatology. Erythropoietin (Epo) enhances endogenous mechanisms of repair and angiogenesis. In order to analyse the newest evidence on the role of Epo in prematurity, hypoxic ischemic encephalopathy (HIE) and perinatal stroke, a critical review using 2020 PRISMA statement guidelines was conducted. This review uncovered 26 clinical trials examining the use of Epo for prematurity and brain injury-related outcomes. The effects of Epo on prematurity were analysed in 16 clinical trials. Erythropoietin was provided until 32–35 weeks of corrected postnatal age with a dosage between 500–3000 UI/kg/dose. Eight trials reported the Epo effects on HIE term newborn infants: Erythropoietin was administered in the first weeks of life, at different multiple doses between 250–2500 UI/kg/dose, as either an adjuvant therapy with hypothermia or a substitute for hypothermia. Two trials investigated Epo effects in perinatal stroke. Erythropoietin was administered at a dose of 1000 IU/kg for three days. No beneficial effect in improving morbidity was observed after Epo administration in perinatal stroke. A positive effect on neurodevelopmental outcome seems to occur when Epo is used as an adjuvant therapy with hypothermia in the HIE newborns. Administration of Epo in preterm infants still presents inconsistencies with regard to neurodevelopmental outcome. Clinical trials show significant differences mainly in target population and intervention scheme. The identification of specific markers and their temporal expression at different time of recovery after hypoxia-ischemia in neonates might be implemented to optimize the therapeutic scheme after hypoxic-ischemic injury in the developing brain. Additional studies on tailored regimes, accounting for the risk stratification of brain damage in newborns, are required.
Transcriptomic Analysis of E. coli after Exposure to a Sublethal Concentration of Hydrogen Peroxide Revealed a Coordinated Up-Regulation of the Cysteine Biosynthesis Pathway
Authors: Roth Myriam , Jaquet Vincent , Lemeille Sylvain , Bonetti Eve-Julie , Cambet Yves , François Patrice , Krause Karl-Heinz
Published: March 2022

Hydrogen peroxide (H2O2) is a key defense component of host-microbe interaction. However, H2O2 concentrations generated by immune cells or epithelia are usually insufficient for bacterial killing and rather modulate bacterial responses. Here, we investigated the impact of sublethal H2O2 concentration on gene expression of E. coli BW25113 after 10 and 60 min of exposure. RNA-seq analysis revealed that approximately 12% of bacterial genes were strongly dysregulated 10 min following exposure to 2.5 mM H2O2. H2O2 exposure led to the activation of a specific antioxidant response and a general stress response. The latter was characterized by a transient down-regulation of genes involved in general metabolism, such as nucleic acid biosynthesis and translation, with a striking and coordinated down-regulation of genes involved in ribosome formation, and a sustained up-regulation of the SOS response. We confirmed the rapid transient and specific response mediated by the transcription factor OxyR leading to up-regulation of antioxidant systems, including the catalase-encoding gene (katG), that rapidly degrade extracellular H2O2 and promote bacterial survival. We documented a strong and transient up-regulation of genes involved in sulfur metabolism and cysteine biosynthesis, which are under the control of the transcription factor CysB. This strong specific transcriptional response to H2O2 exposure had no apparent impact on bacterial survival, but possibly replenishes the stores of oxidized cysteine and glutathione. In summary, our results demonstrate that different stress response mechanisms are activated by H2O2 exposure and highlight the cysteine synthesis as an antioxidant response in E. coli.
BRD4 Targets the KEAP1-Nrf2-G6PD Axis and Suppresses Redox Metabolism in Small Cell Lung Cancer
Authors: Lv Yang , Lv Xiaotong , Zhang Jiahui , Cao Guozhen , Xu Changzhi , Zhang Buchang , Lin Wenchu
Published: March 2022

Accumulating evidence has witnessed the Kelch-like ECH-associated protein 1(KEAP1)- nuclear factor (erythroid-derived 2)-like 2 (Nrf2) axis is the main regulatory factor of cell resistance to endogenous and exogenous oxidative assaults. However, there are few studies addressing the upstream regulatory factors of KEAP1. Herein, bioinformatic analysis suggests bromodomain-containing protein 4 (BRD4) as a potential top transcriptional regulator of KEAP1 in lung cancer. Using molecular and pharmacological approaches, we then discovered that BRD4 can directly bind to the promoter of KEAP1 to activate its transcription and down-regulate the stability of Nrf2 which in turn transcriptionally suppresses glucose-6-phosphate dehydrogenase (G6PD) in small cell lung cancer (SCLC), a highly proliferative and aggressive disease with limited treatment options. In addition, BRD4 could associate with the Nrf2 protein in a non-KEAP1-dependent manner to inhibit Nrf2 activity. Furthermore, simultaneous application of JQ1 and ATRA or RRx-001 yielded synergistic inhibition both in vitro and in vivo. These data suggest metabolic reprogramming by JQ1 treatment improves cell resistance to oxidative stress and might be a resistance mechanism to bromodomain and extra-terminal domain (BET) inhibition therapy. Altogether, our findings provide novel insight into the transcriptional regulatory network of BRD4 and KEAP1 and transcriptional regulation of the pentose phosphate pathway in SCLC.
A Particle Swarm Optimization Algorithm with Sigmoid Increasing Inertia Weight for Structural Damage Identification
Authors: Chen Zhen , Wang Yaru , Chan Tommy H. T. , Li Xiaoke , Zhao Shunbo
Published: March 2022

In this study, a particle swarm optimization with a sigmoid increasing inertia weight (SIPSO) algorithm is proposed for structural damage identification based on the optimization of structural vibration response constraints. In view of the existing problems for particle swarm optimization algorithms used for structural damage identification, such as low accuracy of damage identification and easy misjudgment of damage location, the sigmoid increasing inertia weight is introduced to improve the global and local search ability of the algorithm. Simulation results show that the parameters of the sigmoid increasing inertia weight have a significant effect on the performance of the SIPSO algorithm for structural damage identification. Compared with similar improved particle swarm optimization algorithms, the SIPSO algorithm has some advantages of fast convergence speed, high identification accuracy, and strong robustness ability in structural damage identification.
Hybrid Dilated and Recursive Recurrent Convolution Network for Time-Domain Speech Enhancement
Authors: Song Zhendong , Ma Yupeng , Tan Fang , Feng Xiaoyi
Published: March 2022

In this paper, we propose a fully convolutional neural network based on recursive recurrent convolution for monaural speech enhancement in the time domain. The proposed network is an encoder-decoder structure using a series of hybrid dilated modules (HDM). The encoder creates low-dimensional features of a noisy input frame. In the HDM, the dilated convolution is used to expand the receptive field of the network model. In contrast, the standard convolution is used to make up for the under-utilized local information of the dilated convolution. The decoder is used to reconstruct enhanced frames. The recursive recurrent convolutional network uses GRU to solve the problem of multiple training parameters and complex structures. State-of-the-art results are achieved on two commonly used speech datasets.
Does Nanosilver Have a Pronounced Toxic Effect on Humans?
Authors: Ivlieva Alexandra , Petritskaya Elena , Rogatkin Dmitriy , Yushin Nikita , Grozdov Dmitrii , Vergel Konstantin , Zinicovscaia Inga
Published: March 2022

Due to the development of high-tech industries, the modern world is characterized by the increased production and consumption of nanoparticles (NPs) and nanomaterials. Among produced metal nanoparticles, silver nanoparticles are widely used in everyday life products, cosmetics, and medicine. It has already been established that, in nanoscale form, many even inert materials become toxic. Therefore, the study of the toxicity of various substances in nanoscale form is an urgent scientific task. There is now a body of experience on the toxic effect of AgNPs. In the present review, the most well-known results obtained over the 2009–2021 period, including the own performance on the toxicity of silver NPs, are collected and analyzed. Along with the data reporting a certain level of toxicity of silver NPs, experiments that did not reveal any obvious toxicity of nanosized forms of silver are discussed. According to the performed studies, the toxicity of silver NPs is often caused not by NPs themselves but by silver ions, compounds used for nanoparticle stabilization, and other reasons. Based on the analysis of the collected data, it can be concluded that at actual levels of silver NPs used in everyday life, workplace, and medicine, they will not have strong toxic effects on a healthy adult body.