bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in Quality and Collagen Properties of Cattle Rumen Smooth Muscle Subjected to Repeated Freeze—Thaw Cycles
Authors: Cao Yinjuan , Song Zhaoyang , Han Ling , Yu Qunli , Kong Xiangying , Li Shibao
Published: October 2022

This study revealed changes in the quality, structural and functional collagen properties of cattle rumen smooth muscle (CSM) during F-T cycles. The results showed that thawing loss, pressing loss, β-galactosidase, β-glucuronidase activity, β-sheet content, emulsifying activity index (EAI), emulsion stability index (ESI), surface hydrophobicity, and turbidity of samples were significantly (p < 0.05) increased by 108.12%, 78.33%, 66.57%, 76.60%, 118.63%, 119.57%, 57.37%, 99.14%, and 82.35%, respectively, with increasing F-T cycles. Meanwhile, the shear force, pH, collagen content, α-helix content, thermal denaturation temperature (Tmax), and enthalpy value were significantly (p < 0.05) decreased by 30.88%, 3.19%, 33.23%, 35.92%, 10.34% and 46.51%, respectively. Scanning electron microscopy (SEM) and SDS-PAGE results indicated that F-T cycles induced an increase in disruption of CSM muscle microstructure and degradation of collagen. Thus, repeated F-T cycles promoted collagen degradation and structural disorder in CSM, while reducing the quality of CSM, but improving the functional collagen properties of CSM. These findings provide new data support for the development, processing, and quality control of CSM.
Protective Effect of the Polysaccharides from Taraxacum mongolicum Leaf by Modulating the p53 Signaling Pathway in H22 Tumor-Bearing Mice
Authors: Chen Pei , Chen Yi , Yan Zhi-Qian , Ding Su-Yun , Liu Hui-Ping , Tu Jian-Qiu , Zhang Xiao-Wei
Published: October 2022

Dandelion is an edible plant with a variety of bioactive components. This paper mainly reports the antitumor activity of dandelion polysaccharide DLP120 on H22 tumor-bearing mice. DLP120 is an acidic polysaccharide composed of pectin and arabinogalactan. The results indicate that DLP120 markedly inhibited tumor growth in a dose-dependent manner and attenuated and regulated negative effects on organs. In addition, DLP120 not only increased the viability of spleen lymphocytes and natural killer (NK) cells, but also increased the proportion of lymphocyte subsets in peripheral blood. Furthermore, Hematoxylin-Eosin (HE) staining showed that tumor tissues and cells exhibited typical pathology features. Annexin V FITC/PI staining and cell cycle distribution results further confirmed apoptosis and cell cycle arrest in S and G2 phases. Notably, there was a significant accumulation of reactive oxygen species. Western blotting results demonstrated that the expression of p53 was up-regulated in the DLP120 group. Moreover, the pro-apoptotic protein Bax was up-regulated while the inhibitory-apoptotic protein Bcl-2 was down-regulated. In addition, the expression of Fas and FasL, associated with the death receptor pathway, were also up-regulated. Overall, administration of DLP120 in H22 tumor-bearing mice can not only enhance immunity but also directly induce tumor cell apoptosis.
Changes in Quality of Cold-Pressed Rapeseed Oil with Sinapic Acid Ester-Gelatin Films during Storage
Authors: Rabiej-Kozioł Dobrochna , Tymczewska Alicja , Szydłowska-Czerniak Aleksandra
Published: October 2022

In recent years, cold-pressed rapeseed oil (CPRO) has become an attractive health-beneficial product and a promising alternative to olive oil. However, a high level of unsaturated fatty acids renders a CPRO more susceptible to oxidative deterioration. Therefore, the effect of new gelatin strips with polyvinyl alcohol (GEL-PVA) and sinapic acid esters (ethyl sinapate—ESA; octyl sinapate—OSA, and cetyl sinapate—CSA) on the oxidative stability, antioxidant activity (AA), and total phenolic content (TPC) in CPRO samples was analyzed during accelerated storage. In addition, the antioxidant properties of the GEL-PVA films loaded with sinapic acid esters were studied. Interestingly, the CPRO stored in an amber glass marasca bottle containing GEL-PVA-ESA strips for 14 days had the highest AA, while the antioxidant potential was the lowest for oil without film strips. Furthermore, oil samples stored in bottles containing GEL-PVA-ESA and GEL-PVA-OSA strips for 14 days had higher antioxidant properties than the AA and TPC in fresh CPRO. Moreover, synchronous fluorescence (SF) spectroscopy and excitation-emission matrix (EEM) fluorescence spectroscopy were applied for the evaluation of changes in the main fluorescent components in CPRO samples during the accelerated storage. Overall, this study revealed that GEL-PVA films incorporated with sinapic acid esters enhanced the antioxidant potential of CPRO and delayed oxidative degradation by releasing amphiphilic antioxidants into the oil.
Microencapsulated Healthy Oil Mixtures to Enhance the Quality of Foal Pâtés
Authors: Cittadini Aurora , Munekata Paulo E. S. , Pateiro Mirian , Sarriés María V. , Domínguez Rubén , Lorenzo José M.
Published: October 2022

This study aimed to evaluate the use of microencapsulated oil mixtures as partial animal fat replacers and their effects on the physicochemical, nutritional and sensory qualities of foal pâtés. Three different batches were manufactured: a control (CON) formulation, with foal dorsal subcutaneous fat (30 g/100 g), and treatments 1 and 2 (T1 and T2), with 50% of the animal fat replaced by microcapsules containing algal oil mixed with walnut oil (T1) or pistachio oil (T2). The reformulated samples presented significant (p < 0.001) diminutions of fat contents, which achieved reductions of 34.22% (“reduced fat content”) and 28.17% in the T1 and T2 samples, respectively, and the lipid reformulation did not affect (p > 0.05) the texture or lipid oxidation of the samples. Furthermore, both microencapsulated oil mixtures significantly (p < 0.001) reduced (11–15%) saturated fatty acid (SFA) concentrations and increased (p < 0.001) mono- (T2) and polyunsaturated (T1) fatty acid contents (8% and 68%, respectively), contributing to the obtainment of nutritional indices in line with health recommendations. Additionally, consumer acceptability did not display significant (p > 0.05) differences among samples. Hence, the outcomes indicated that the incorporation of these microencapsulated oil mixtures as partial animal fat replacers, especially the T1 mixture, represents a promising strategy to obtain healthier foal pâtés, without compromising consumer approval.
Functional Integro-Differential Equations with State-Dependent Delay and Non-Instantaneous Impulsions: Existence and Qualitative Results
Authors: Bensalem Abdelhamid , Salim Abdelkrim , Benchohra Mouffak , N’Guérékata Gaston M.
Published: October 2022

This paper addresses some existence, attractivity and controllability results for semilinear integrodifferential equations having non-instantaneous impulsions on an infinite interval via resolvent operators in case of neutral and state-dependent delay problems. Our criteria were obtained by applying a Darbo’s fixed-point theorem combined with measures of noncompactness. The obtained result is illustrated by an example at the end.
Efficacy of Helicobacter pylori Eradication Based on Rabeprazole–Bismuth–Tetracycline–Tinidazole Regimen in Vietnamese Patients with Duodenal Ulcers
Authors: Nguyen Liem Thanh , Nguyen Vuong Ba , Tran Tu Viet , Duong Huy Quang , Le Loan Thi Thuy , Phuong Mai Huynh Truc , Nguyen Thang
Published: October 2022

(1) Background: In Vietnam, H. pylori bacteria has a resistance rate of 63% to the antibiotic clarithromycin. The initial therapy of H. pylori eradication with a standard three-drug regimen has low efficacy. Objective: Assess the efficacy of H. pylori eradication therapy which uses a four-drug regimen of rabeprazole–bismuth–tetracycline–tinidazole in patients with duodenal ulcers. (2) Methods: We performed gastrointestinal endoscopy on patients with a diagnosis of duodenal ulcers, gastric mucosa biopsy for a rapid urease test, and histopathology to diagnose H. pylori bacteria before and after treatment. Treatment for eradication of H. pylori bacteria using a rabeprazole–bismuth–tetracycline–tinidazole regimen was prescribed for 14 days. (3) Results: The rate of successful H. pylori eradication treatment according to per protocol (PP) and intention to treat (ITT) was 91.3% (95%CI: 84.8–96.7) and 82.4% (95%CI: 74.5–89.2) respectively. The success rate of H. pylori eradication therapy in males was 96.0% (95%CI: 92–100), higher than in females, which was 70.6% (95%CI: 47.1–88.2), p < 0.01. (4) Conclusions: Treatment of H. pylori with rabeprazole–bismuth–tetracycline–tinidazole regimen is highly effective. Men had higher H. pylori eradication results than women.
Fabrication of Textile Waste Fibers Aerogels with Excellent Oil/Organic Solvent Adsorption and Thermal Properties
Authors: Dong Chunlei , Hu Yangzhao , Zhu Yuxuan , Wang Jiale , Jia Xuerui , Chen Jianbing , Li Jingliang
Published: October 2022

In recent years, the treatment of textile waste has attracted more and more attention around the world. The reuse of textile waste can contribute to the reduction of carbon emissions and the sustainable development of the economy. Herein, we proposed a facile and cost-effective approach to fabricating aerogel by using textile waste fibers as the matrix and polyvinyl alcohol (PVA) and glutaraldehyde (GA) as crosslinking agents. After being modified with methyltrimethoxysilane (MTMS) via chemical vapor deposition, both the interior and exterior of the textile waste aerogels exhibit a hydrophobic property with a water contact angle of up to 136.9° ± 2.3°. A comprehensive investigation of the structure, thermal properties, mechanical properties and oil absorption capacity of this aerogel shows its potential for building insulation and oil spill cleanup. The textile waste fibers aerogels have low density and high porosity, good thermal stability and outstanding heat insulation properties (Kavg. = 0.049–0.061 W/m·K). With a maximum oil absorption value of 26.9 ± 0.6 g/g and rapid and effective oil/water mixture separation, the aerogel exhibits competitive commercial application value.
Divided Loyalties: Negotiating Marital Separation in the Cavendish-Talbot Family c.1575–90
Authors: Cannon Maria
Published: October 2022

Bringing together two areas of scholarship on family history—separation and blended families—this article adds a new perspective to our understanding of how kin networks in early modern England were maintained, and on the factors that influenced the ongoing processes of negotiating them. The extensive correspondence of Bess of Hardwick and her children and step-children enables an investigation into what happened when a couple at the centre of a blended family network separated. Despite their unique political circumstances, the Cavendish-Talbot family offer a useful case study to understand some of the factors shaping the lives of separated wives in early modern England. For elite families the success of the house and dynasty could be jeopardized by the breakdown of a marriage, and never more so than if the family was a blended one. While Bess’s relationships with and support for her children caused problems with her husband, their invaluable support indicates further strategies that were available to separated wives. Bess’s children advocated for her at court, supported her in legal suits and actively negotiated between their parents. The Cavendish-Talbot family relationships were complex and loyalties did not necessarily follow expected patterns. However, in their complexity, and through the large number of letters surviving between the family, they offer a unique opportunity to consider the role of family members for separated wives.
Classification and Prognosis Analysis of Pancreatic Cancer Based on DNA Methylation Profile and Clinical Information
Authors: Li Xin , Zhang Xuan , Lin Xiangyu , Cai Liting , Wang Yan , Chang Zhiqiang
Published: October 2022

Pancreatic adenocarcinoma (PAAD) has a poor prognosis with high individual variation in the treatment response among patients; however, there is no standard molecular typing method for PAAD prognosis in clinical practice. We analyzed DNA methylation data from The Cancer Genome Atlas database, which identified 1235 differentially methylated DNA genes between PAAD and adjacent tissue samples. Among these, 78 methylation markers independently affecting PAAD prognosis were identified after adjusting for significant clinical factors. Based on these genes, two subtypes of PAAD were identified through consistent clustering. Fourteen specifically methylated genes were further identified to be associated with survival. Further analyses of the transcriptome data identified 301 differentially expressed cancer driver genes between the two PAAD subtypes and the degree of immune cell infiltration differed significantly between the subtypes. The 14 specific genes characterizing the unique methylation patterns of the subtypes were used to construct a Bayesian network-based prognostic prediction model for typing that showed good predictive value (area under the curve value of 0.937). This study provides new insight into the heterogeneity of pancreatic tumors from an epigenetic perspective, offering new strategies and targets for personalized treatment plan evaluation and precision medicine for patients with PAAD.
The Light Chain Domain and Especially the C-Terminus of Receptor-Binding Domain of the Botulinum Neurotoxin (BoNT) Are the Hotspots for Amino Acid Variability and Toxin Type Diversity
Authors: Tian Renmao , Widel Melissa , Imanian Behzad
Published: October 2022

Botulinum neurotoxins (BoNT) are the most potent toxins in the world. They are produced by a few dozens of strains within several clostridial species. The toxin that they produce can cause botulism, a flaccid paralysis in humans and other animals. With seven established serologically different types and over 40 subtypes, BoNTs are among the most diverse known toxins. The toxin, its structure, its function and its physiological effects on the neural cell and animal hosts along with its diversity have been the subjects of numerous studies. However, many gaps remain in our knowledge about the BoNT toxin and the species that produce them. One of these gaps involves the distribution and extent of variability along the full length of the gene and the protein as well as its domains and subdomains. In this study, we performed an extensive analysis of all of the available 143 unique BoNT-encoding genes and their products, and we investigated their diversity and evolution. Our results indicate that while the nucleotide variability is almost uniformly distributed along the entire length of the gene, the amino acid variability is not. We found that most of the differences were concentrated along the protein’s light chain (LC) domain and especially, the C-terminus of the receptor-binding domain (HCC). These two regions of the protein are thus identified as the main source of the toxin type differentiation, and consequently, this toxin’s versatility to bind different receptors and their isoforms and act upon different substrates, thus infecting different hosts.
Integration of lncRNAs, Protein-Coding Genes and Pathology Images for Detecting Metastatic Melanoma
Authors: Liu Shuai , Fan Yusi , Li Kewei , Zhang Haotian , Wang Xi , Ju Ruofei , Huang Lan , Duan Meiyu , Zhou Fengfeng
Published: October 2022

Melanoma is a lethal skin disease that develops from moles. This study aimed to integrate multimodal data to predict metastatic melanoma, which is highly aggressive and difficult to treat. The proposed EnsembleSKCM method evaluated the prediction performances of long noncoding RNAs (lncRNAs), protein-coding messenger genes (mRNAs) and pathology images (images) for metastatic melanoma. Feature selection was used to screen for metastatic biomarkers in the lncRNA and mRNA datasets. The integrated EnsembleSKCM model was built based on the weighted results of the lncRNA-, mRNA- and image-based models. EnsembleSKCM achieved 0.9444 in the prediction accuracy of metastatic melanoma and outperformed the single-modal prediction models based on the lncRNA, mRNA and image data. The experimental data suggest the importance of integrating the complementary information from the three data modalities. WGCNA was used to analyze the relationship of molecular-level features and image features, and the results show connections between them. Another cohort was used to validate our prediction.
POLDIP3: At the Crossroad of RNA and DNA Metabolism
Authors: Singh Manrose , Zhang Sufang , Perez Alexis M. , Lee Ernest Y. C. , Lee Marietta Y. W. T. , Zhang Dong
Published: October 2022

POLDIP3 was initially identified as a DNA polymerase delta (Pol δ) interacting protein almost twenty years ago. Intriguingly, it also interacts with proteins involved in a variety of RNA related biological processes, such as transcription, pre-mRNA splicing, mRNA export, and translation. Studies in recent years revealed that POLDIP3 also plays critical roles in disassembling genome wide R-loop formation and activating the DNA damage checkpoint in vivo. Here, we review the functions of POLDIP3 in various RNA and DNA related cellular processes. We then propose a unified model to illustrate how POLDIP3 plays such a versatile role at the crossroad of the RNA and DNA metabolism.
Genetic Hearing Loss Affects Cochlear Processing
Authors: Lanting Cris , Snik Ad , Leijendeckers Joop , Bosman Arjan , Pennings Ronald
Published: October 2022

The relationship between speech recognition and hereditary hearing loss is not straightforward. Underlying genetic defects might determine an impaired cochlear processing of sound. We obtained data from nine groups of patients with a specific type of genetic hearing loss. For each group, the affected cochlear site-of-lesion was determined based on previously published animal studies. Retrospectively obtained speech recognition scores in noise were related to several aspects of supra-threshold cochlear processing as assessed by psychophysical measurements. The differences in speech perception in noise between these patient groups could be explained by these factors and partially by the hypothesized affected structure of the cochlea, suggesting that speech recognition in noise was associated with a genetics-related malfunctioning of the cochlea. In particular, regression models indicate that loudness growth and spectral resolution best describe the cochlear distortions and are thus a good biomarker for speech understanding in noise.
Genotyping of Coxiella burnetii from Cattle by Multispacer Sequence Typing and Multiple Locus Variable Number of Tandem Repeat Analysis in the Republic of Korea
Authors: Truong A-Tai , Youn So Youn , Yoo Mi-Sun , Lim Ji-Yeon , Yoon Soon-Seek , Cho Yun Sang
Published: October 2022

Genotyping of Coxiella burnetii using multispacer sequence typing (MST) and multiple locus variable number tandem repeat analysis (MLVA) was conducted from infected animals for the first time in the Republic of Korea. C. burnetii was detected by real-time PCR, and followed by MST and MLVA genotyping. The result showed that detected C. burnetii all had the same MLVA genotype, 6-13-2-7-9-10 for markers MS23-MS24-MS27-MS28-MS33-MS34, respectively, and genotype group 61 for MST. The same genotypes were previously identified in Poland. Importantly, this MLVA type was detected in humans in France, suggesting that the Korean strain can also potentially cause Q fever in humans. MST and MLVA were very useful tools for analyzing the molecular epidemiology of C. burnetii and helpful for interpreting the epidemiological relationship between isolates from domestic and international resources.
Integrated Tissue and Blood miRNA Expression Profiles Identify Novel Biomarkers for Accurate Non-Invasive Diagnosis of Breast Cancer: Preliminary Results and Future Clinical Implications
Authors: Su Fei , Gao Ziyu , Liu Yueyang , Zhou Guiqin , Cui Ying , Deng Chao , Liu Yuyu , Zhang Yihao , Ma Xiaoyan , Wang Yongxia , Guan Lili , Zhang Yafang , Liu Baoquan
Published: October 2022

We aimed to identify miRNAs that were closely related to breast cancer (BRCA). By integrating several methods including significance analysis of microarrays, fold change, Pearson’s correlation analysis, t test, and receiver operating characteristic analysis, we developed a decision-tree-based scoring algorithm, called Optimized Scoring Mechanism for Primary Synergy MicroRNAs (O-PSM). Five synergy miRNAs (hsa-miR-139-5p, hsa-miR-331-3p, hsa-miR-342-5p, hsa-miR-486-5p, and hsa-miR-654-3p) were identified using O-PSM, which were used to distinguish normal samples from pathological ones, and showed good results in blood data and in multiple sets of tissue data. These five miRNAs showed accurate categorization efficiency in BRCA typing and staging and had better categorization efficiency than experimentally verified miRNAs. In the Protein-Protein Interaction (PPI) network, the target genes of hsa-miR-342-5p have the most regulatory relationships, which regulate carcinogenesis proliferation and metastasis by regulating Glycosaminoglycan biosynthesis and the Rap1 signaling pathway. Moreover, hsa-miR-342-5p showed potential clinical application in survival analysis. We also used O-PSM to generate an R package uploaded on github (SuFei-lab/OPSM accessed on 22 October 2021). We believe that miRNAs included in O-PSM could have clinical implications for diagnosis, prognostic stratification and treatment of BRCA, proposing potential significant biomarkers that could be utilized to design personalized treatment plans in BRCA patients in the future.
Lifestyle Strategies for the Management of Obesity in Older Adults: From Controversies to Alternative Interventions
Authors: Villani Anthony
Published: October 2022

Improvements in infection control, management of chronic diseases and impressive advancements in modern medicine have all contributed to increases in life expectancy [...]
Clothes Make the Man—What Impact Does the Dress of Interprofessional Teams Have on Patients?
Authors: Roehrens Hans Joachim , Ehlers Jan P. , Zupanic Michaela
Published: October 2022

Physicians’ attire seems to play an important role in the success of patient treatment. The classic doctor’s white coat initiates a strong signal to the patient and can have a determining effect on a successful doctor–patient relationship. In a quantitative online questionnaire study comprising 52 questions, participants were shown four photos of an interprofessional German family medicine team in varying attire. One important study feature relating to the ongoing coronavirus pandemic was that the team was portrayed wearing FFP2 masks in one photo. We measured core values regarding the team’s perception in terms of sympathy, competence, trust, choosing the practice as a personal health care provider, and wanting to participate in the team. The questionnaire was posted online between March and May 2021. It was accessed 1435 times and 906 sheets were qualified for statistical analysis. For the first time in this field of research, a practice team’s attire was investigated. We found a significant influence of different clothing on the perception of sympathy, competence, trust, elective practice, and team participation. Wearing an FFP2 mask promotes feelings of security and competence. The study shows that in times of fast social changes due to rapid digitalization and an ongoing pandemic, we should present ourselves in different ways as a medical team depending on the patient groups we are targeting and the feelings we want to evoke.
A Study on Determinants of COVID-19 Preventive Health Behaviors of Mothers with Young Children in South Korea
Authors: An Hye-Gyung , Yoon Chae-Min
Published: October 2022

This study is a descriptive research study conducted to identify factors that affect children of Korean mothers the coronavirus disease 2019 (COVID-19) preventive health behavior. It was confirmed that knowledge of COVID-19, maternal confidence, and risk perception of COVID-19 infection were related to the Preventive health behaviors of COVID-19 of Korean mothers with children. The subjects of this study were 191 mothers residing in Korea and raising children under the age of 5, and data were collected through an online questionnaire. We used the Google platform to fill out a questionnaire and collect data using a network sampling method from mothers who voluntarily participated in a survey at an online community meeting of mothers with young children. The collected data were analyzed using descriptive statistics, factor analysis, t-test, Pearson’s correlation analysis, and multiple regression analysis using IBM SPSS statistics 21.0 program. Preventive health behaviors of COVID-19 factor 1 are positively correlated with: mothers’ COVID-19 knowledge (r = 0.192, p < 0.01), confidence in infant care knowledge(r = 0.179, p < 0.05), and satisfaction with the role of mother(r = 0.351, p < 0.001). Negatively correlated with: unacceptable risk perception(r = −0.222, p< 0.01). Preventive health behaviors of COVID-19 factor 2 are positively correlated with: mothers’ COVID-19 knowledge (r = 0.166, p < 0.05), confidence in infant care knowledge(r = 0.179, p < 0.05), and satisfaction with the role of mother(r = 0.338, p < 0.001). Negatively correlated with: unacceptable risk perception(r = −0.205, p < 0.01). To strengthen COVID-19 preventive health behavior of Korean mothers with young children, it is suggested that education programs should be developed to provide accurate knowledge, increase maternal confidence, and improve the risk perception of COVID-19 infection.
Development and Validation of the First Smart TV-Based Visual Acuity Test: A Prospective Study
Authors: Labiris Georgios , Delibasis Konstantinos , Panagiotopoulou Eirini-Kanella , Pigadas Vassilis , Bakirtzis Minas , Panagis Christos , Dardabounis Doukas , Ntonti Panagiota
Published: October 2022

(1) Background: While smartphones are among the primary devices used in telemedical applications, smart TV healthcare apps are not prevalent despite smart TVs’ penetrance in home settings. The present study’s objective was to develop and validate the first smart TV-based visual acuity (VA) test (Democritus Digital Visual Acuity Test (DDiVAT)) that allows a reliable VA self-assessment. (2) Methods: This is a prospective validation study. DDiVAT introduces several advanced features for reliable VA self-testing; among them: automatic calibration, voice recognition, voice guidance, automatic calculation of VA indexes, and a smart TV-based messaging system. Normal and low vision participants were included in the validation. DDiVAT VA results (VADDiVAT) were compared against the ones from: (a) the gold-standard conventional ETDRS (VAETDRS), and, (b) an independent ophthalmologist who monitored the self-examination testing (VARES). Comparisons were performed by noninferiority test (set at 2.5-letters) and intraclass correlation coefficients (ICCs). DDiVAT’s test-retest reliability was assessed within a 15-day time-window. (3) Results: A total of 300 participants (185 and 115 with normal and low vision, respectively) responded to ETDRS and DDiVAT. Mean difference in letters was −0.05 for VAETDRS–VARES, 0.62 for VARES–VADDiVAT, and 0.67 for VAETDRS–VADDiVAT, significantly lower than the 2.5 letter noninferiority margin. ICCs indicated an excellent level of agreement, collectively and for each group (0.922-0.996). All displayed letters in DDiVAT presented a similar difficulty. The overall accuracy of the voice recognition service was 96.01%. ICC for VADDiVAT test-retest was 0.957. (4) Conclusions: The proposed DDiVAT presented non-significant VA differences with the ETDRS, suggesting that it can be used for accurate VA self-assessment in telemedical settings, both for normal and low-vision patients.
A Model for Effective Nonverbal Communication between Nurses and Older Patients: A Grounded Theory Inquiry
Authors: Wanko Keutchafo Esther L. , Kerr Jane , Baloyi Olivia B.
Published: October 2022

Nonverbal communication is an inevitable art to be effectively mastered by nurses. Nurse nonverbal communication has many benefits when it is effective. For instance, nonverbal communication is important to convey affective and emotional information, and demonstrate respect for and build therapeutic relationships with older patients. As the older population is growing fast worldwide, effective nonverbal communication with older patients is an essential skill for nurses and will improve patients’ satisfaction and the quality of care. Therefore, this article presents a model to guide effective nonverbal communication between nurses and older patients. A Grounded Theory approach guided the study. Data were collected between July 2018 and January 2020 through overt participant observations and individual interviews. Purposive and theoretical sampling were used to select 13 clinically experienced nurses, 4 nursing students, and 8 older adults. Data analysis encompassed open coding, axial coding, and selective coding. The results showed that effective nonverbal communication emerged as the co-phenomenon hinged within context and/or environment and is influenced by certain factors. This model, which is in support of person-centered communication and care, advocates for effective nonverbal communication between nurses and older patients.
A Comparison of IPM and Organic Farming Systems Based on the Efficiency of Oophagous Predation on the Olive Moth (Prays oleae Bernard) in Olive Groves of Southern Iberia
Authors: Gómez-Guzmán José Alfonso , Herrera José M. , Rivera Vanesa , Barreiro Sílvia , Muñoz-Rojas José , García-Ruiz Roberto , González-Ruiz Ramón
Published: October 2022

The olive moth, Prays oleae (Bernard, 1788) (Lep., Praydidae), is one of the most common insect pests affecting the olives groves of the Mediterranean basin. Current farming practices are largely oriented to optimize the effectiveness of beneficial insects, among which the common green lacewings (Neur., Chrysopidae) stand out. Two different types of management models, organic and IPM, were compared in this study, which was conducted in olive groves in the regions of Andalucía (Spain) and Alentejo (Portugal). During 2020 and 2021, fruit samples were periodically collected, analyzing the population parameters (POP) and potential attack on the fruit (%PA), as well as the predatory impact (%PRED), which has allowed the estimation of the final attack (%FA), and derived fruit recovery rates (%REC). The results show that in organic olive groves of both countries, the infestation parameters (POP, %PA) were significantly higher than in IPM ones. However, predation rates were also higher in organic olive groves, which resulted in REC rates of between 75% and 80%, reducing FA rates to values of approximately 10% and 20% in Portugal and Spain, respectively. In contrast, in the IPM olive groves, significantly lower predation values were recorded, with lower REC rates than in the organic olive groves; the rates were very similar in both countries (ca. 54%), which led to a higher percentage of fruit loss (%FA) equivalent to 22% (Portugal) and to 34% (Spain). This paper discusses potential drivers influencing differences in the population values and percentages of infestation by P. oleae observed, as well as the differences in the final attack rates between olive groves of both countries, subject to the same type of agricultural management.
Phenotypic and Chemotypic Relations among Local Andrographis paniculata (Burm. f.) Wall Landrace Collection
Authors: Onsa Nuttacha Eva , Prasad Shashanka K. , Chaiyaso Thanongsak , Lumsangkul Chompunut , Sommano Sarana Rose
Published: October 2022

The relationship between the phenotypic and chemical composition of local Andrographis paniculata was evaluated in this study. Five seed collections were sourced from different regions of Thailand, namely Kamphaeng Saen (KS), Udon Thani (UT), Chiang Rai (CR), Chiang Mai (CM), and Ratchaburi (RB). They were cultivated in the same conditions, potted, and partially shaded (60%) in an open conventional greenhouse. The phenology and chemical composition of these plants were assessed at the commercial harvesting stage (ca. 90 days after planting). The results indicated that UT was morphologically distinctive, illustrating the highest edible biomass yield (aerial and mature leaf size). The above-ground parts (viz., leaves and stem) were then analyzed for bioactive compounds after maceration with 80% (w/w) ethanol. It was found that the highest lactone content (~14 mg/g extract) was obtained from leaf and stem extracts of all samples except KS. Nonetheless, total phenolics and flavonoids in the stem extract of KS were found to be the highest at 3.22 and 2.42 mg/g, respectively. Phytochemicals from both leaf and stem extracts were capable of high anti-oxidant activity (~70%) as determined by DPPH and ABTS assays. Chemically, RB contained the highest 14-deoxy-11,12-didehydroandrographolide (156.98 mg/g extract), while UT and CM contained up to 0.68 mg/g extract of neoandrographolide. Classification of the samples indicated a clear relationship between the morphological traits and chemical compositions. In conclusion, our findings suggest the variations in phenotypic and chemotypic relations across the different landraces of A. paniculata. In essence, the quantity of the consumable parts was essentially the marker to describe the quality of the phytochemical constituents. The overall outcome of this study was to select the physiological characteristics that could be used for further breeding programs of the ideal variety with high productivity and higher bioactive(s) content.
Antifungal Activity of Ginger Rhizome Extract against Fusarium solani
Authors: Xi Ke-Yong , Xiong Shi-Jie , Li Gang , Guo Chang-Quan , Zhou Jie , Ma Jia-Wei , Yin Jun-Liang , Liu Yi-Qing , Zhu Yong-Xing
Published: October 2022

Fusarium solani is one of the most ruinous soil-borne pathogens and seriously affects crop yields and quality worldwide. Ginger is an important medicinal crop, and ginger rhizome extract (GRE) has been used as an alternative for chemical fungicides and bactericides. We investigated the fungistatic effect of GRE on F. solani and analyzed the chemical constituents of GRE using UPLC-MS/MS. Antifungal assay results showed that 20 mg/mL of GRE completely inhibited the growth of F. solani. Morphological analysis revealed that GRE destroyed the morphology and structure of mycelia, thus inhibiting mycelial growth. Furthermore, GRE suppressed the activities of cell wall-degrading and cellular respiratory-related enzymes and decreased the content of fusaric acid, which reduced or even abrogated the infection ability of F. solani. UPLC-MS/MS analysis showed that GRE constituents belonged to eight categories, among which phenolic acids were the highest in content (46.29%) and tannins were the lowest in content (0.06%). When the antifungal activities of major phenolic and flavonoid compounds were evaluated, 4.0 mg/mL 4-hydroxybenzaldehyde and 15.0 mg/mL quercetin were found to completely inhibit F. solani growth. These results highlight GRE as an excellent source of antifungal compounds and suggest the possibility of using 4-hydroxybenzaldehyde and quercetin as natural fungicides to control crop diseases.
Effect of Flower Development Stages on the Dynamics of Volatile Compounds in Ylang-Ylang (Cananga odorata) Essential Oil
Authors: Chakira Abacar , Garcia Cyrielle , Soria Christian , Minier Jérôme , Chillet Marc
Published: October 2022

Several abiotic factors influence the chemical composition of essential oils. Understanding these factors is an important step in developing quality products that meet market demands. This research work aims to study the chemical composition of the essential oils of ylang-ylang (Cananga odorata, forma genuina) according to the maturity of flowers. The volatile compounds of ylang-ylang flowers from Reunion Island were extracted by hydrodistillation and the samples were analyzed by high resolution gas chromatography coupled with mass spectrometry (GC-MS) allowing for the identification of 70 volatile compounds. The chemical composition of the essential oils extracted from the flowers at 5 different stages of development varies both qualitatively and quantitatively. The volatile compounds observed belong to 4 chemical groups which are esters, alcohols, terpenes, and ether-oxides. The synthesis of light oxygenated compounds is largely the predominant chemical subgroup in all stages of development. Their relative content is considerably increased during flower ripening with a peak concentration in stage 4 (SD4). The highest concentrations of non-terpene esters and heavy oxygenated compounds are found in stages 1 (SD1) and (SD5), respectively, while no chemical subgroup is dominant in the intermediate stages 2 (SD2) and (SD3). The dynamics of volatile compounds have also been studied. This study established that the stage of development of ylang-ylang flowers significantly influences the dynamics of volatile compounds in the extracted essential oils. Total oxygenated compounds that are highly odoriferous in essential oils increase progressively and significantly with flower maturity, unlike hydrocarbon terpenes, which are less valuable in terms of their contribution to the fragrance and following opposite kinetics; suggesting that odoriferous properties increase with the development of the flower, with a higher intensity at SD4.
On the Benefits of Collaboration between Decision Makers and Scientists: The Case of Lake Como
Authors: Bertoli Luigi , Balzarolo Donata , Todini Ezio
Published: October 2022

Rational Water Resources Management requires effective collaboration between decision-makers involved in the operational management of water resources and scientists, who can allow them to operate in an informed manner through forecasting and decision-making tools. In this article, we show the potential benefits resulting from this collaboration through the description of the emblematic case of Lake Como. The article describes the real case of a collaborative experience between decision makers, who made an effort to highlight and clarify the real management problems to scientists, who in turn needed to understand all the facets of the decision-making process prior to formulating the problem in mathematical terms and incorporating the solution into a decision support system. The resulting tool, which makes extensive hidden use of probabilistic forecasts, stochastic optimization, and Bayesian decision techniques, resulted in a user-friendly environment. After six months of testing, the tool proved to be essential for decision-making and has been in use on a daily basis since 1997.
Addressing Capacity Constraints of Rural Local Health Departments to Support Climate Change Adaptation: Action Is Needed Now
Authors: Vo Matthew V. , Ebi Kristie L. , Busch Isaksen Tania M. , Hess Jeremy J. , Errett Nicole A.
Published: October 2022

Looming climate change health impacts among rural communities will require a robust health system response. To reduce health inequities and promote climate justice, rural local health departments (LHDs) must be adequately resourced and supported to engage in climate change mitigation and adaptation policy and program development and implementation. In the United States, small local tax bases, overreliance on revenue from fee-based services, and limited federal funding to support climate change and health programming, have left rural LHDs with limited and inflexible human, financial, and political capital to support engagement in local climate change activities. Because of the urgent demands stemming from climate change, additional investments and supports are needed to rapidly build the capacity and capability of rural LHDs. Federal and state approaches to public health funding should consider the unique climate change and health risks of rural communities. Further, cross-jurisdictional shared service arrangements and state-level support to build rural LHDs’ technical capacity, and research on local impacts and culturally appropriate solutions, must be prioritized.
Changes in Fruit and Vegetable Consumption and Leisure Time Physical Exercise after a Citizen Science-Based Worksite Health Promotion Program for Blue-Collar Workers
Authors: van der Feltz Sophie , van der Molen Henk F. , Lelie Lisa , Hulshof Carel T. J. , van der Beek Allard J. , Proper Karin I.
Published: October 2022

Blue-collar workers have, on average, poorer health than white-collar workers. Existing worksite health promotion programs (WHPPs) are often not successful among blue-collar workers. This study evaluates the effect of the Citizen Science-based WHPP on the targeted lifestyle behaviors among construction workers. The data of 114 participants were retrieved from questionnaires before (T0) and after (T1) the WHPP. Outcome measures were mean and categorical changes in daily fruit and vegetable intake and weekly leisure time physical exercise. Changes were tested using Wilcoxon signed rank tests and McNemar tests. No statistically significant changes were found between T0 and T1. In total, 73.7% of the participants felt involved in the WHPP. Changes in the outcome measures were not significantly different between subgroups based on age, nor in subgroups based on feelings of involvedness. The low intensity of the developed program could be an explanation for this lack of significant change. Future studies using the Citizen Science approach in an occupational setting should aim at developing a more intensified program and should test its effectiveness by comparing changes in a (randomized) controlled trial.
Parenting Programme Structure, Potential Barriers, and Facilitators: A Scoping Review
Authors: Ramos Ana , Matos Filomena , Soares Hélia
Published: October 2022

Becoming a parent is a challenging transition, and stress factors may arise. This scoping review aims to map, from the literature, the structure, potential barriers, and facilitators to be considered when conducting a parenting programme for parents of children up to 3 years old. It followed the JBI methodology and included studies with parents of children up to 3 years old (Participants), studies about parenting programme structure, its potential barriers, and facilitators (Concept) in the healthcare or community setting (Context). Qualitative and/or quantitative study designs and grey literature publications between 2016 and 2021 were eligible. The search was performed in three stages in CINAHL Plus with Full Text, MEDLINE with Full Text, and PubMed databases. It was also conducted in OpenGrey, ProQuest, Portuguese Open Access Scientific Repository, and Google Scholar. Fourteen articles were selected. The following aspects were identified regarding parenting programmes: benefits; structuring elements to be considered; facilitating factors and possible barriers to its development, and measurement instruments to assess the programme. Parenting programmes are important ways to contribute to a healthy, sustainable, and resilient society. It should be adapted to individuals, groups, and communities. They add value to parents, children, and society and should be carefully adapted to the group’s needs.
Predicting Transmission Suitability of Mosquito-Borne Diseases under Climate Change to Underpin Decision Making
Authors: Sargent Kate , Mollard James , Henley Sian F. , Bollasina Massimo A.
Published: October 2022

The risk of the mosquito-borne diseases malaria, dengue fever and Zika virus is expected to shift both temporally and spatially under climate change. As climate change projections continue to improve, our ability to predict these shifts is also enhanced. This paper predicts transmission suitability for these mosquito-borne diseases, which are three of the most significant, using the most up-to-date climate change projections. Using a mechanistic methodology, areas that are newly suitable and those where people are most at risk of transmission under the best- and worst-case climate change scenarios have been identified. The results show that although transmission suitability is expected to decrease overall for malaria, some areas will become newly suitable, putting naïve populations at risk. In contrast, transmission suitability for dengue fever and Zika virus is expected to increase both in duration and geographical extent. Although transmission suitability is expected to increase in temperate zones for a few months of the year, suitability remains focused in the tropics. The highest transmission suitability in tropical regions is likely to exacerbate the intense existing vulnerability of these populations, especially children, to the multiple consequences of climate change, and their severe lack of resources and agency to cope with these impacts and pressures. As these changes in transmission suitability are amplified under the worst-case climate change scenario, this paper makes the case in support of enhanced and more urgent efforts to mitigate climate change than has been achieved to date. By presenting consistent data on the climate-driven spread of multiple mosquito-borne diseases, our work provides more holistic information to underpin prevention and control planning and decision making at national and regional levels.
Respiratory Tract and Eye Symptoms in Wildland Firefighters in Two Canadian Provinces: Impact of Discretionary Use of an N95 Mask during Successive Rotations
Authors: Cherry Nicola , Broznitsky Natasha , Fedun Mike , Zadunayski Tanis
Published: October 2022

We examined whether discretionary use of an N95 mask reduced symptom reporting in wildland firefighters. The study collected data from two Canadian provinces during the 2021 fire season, with each firefighter followed for up to 4 rotations. Participants completed questionnaires on symptoms at the start and end of each rotation, when they reported also on mask use (if any) and completed a task checklist. Eighty firefighters contributed data. Nineteen firefighters were successfully fit-tested for N95 masks to wear whenever they felt conditions justified. Start-of-rotation symptoms reflected total hours firefighting in 2021. Symptoms of eye, nose and throat irritation and cough were more bothersome at the end of rotation. Cough, throat and nose (but not eye) symptoms were reported as significantly less bothersome at the end of rotation by those allocated masks, having allowed for crew type and start-of-rotation symptoms. Among those allocated a mask, use was most frequent during initial attack and least during driving and patrol. Reasons for not wearing included high work difficulty and low comfort. It is concluded that symptoms in wildland firefighters increased with hours of exposure. While provision of an N95 mask reduced symptoms, work is needed to overcome barriers to respiratory protection
Comparison between Smokers and Smokeless Tobacco Users in Their Past Attempts and Intentions to Quit: Analysis of Two Rounds of a National Survey
Authors: Islam M. Mofizul
Published: October 2022

This study compares current tobacco smokers and smokeless tobacco (SLT) users in terms of their past quitting attempts and intentions to quit in the future, and identifies approaches used in their recent quitting attempts. Data (n = 14,498) of current tobacco users from two rounds of the Global Adult Tobacco Survey Bangladesh were analysed. Poisson regressions with robust variance were used to examine associations between the study factor and the two outcome variables. About half of smokers and a quarter of SLT users tried to quit during the 12 months before the survey. About two-thirds of smokers and half of SLT users intended to quit in the future. Smokers were more likely (adjusted prevalence ratio (aPR): 1.38, 95%CI: 1.24–1.53) than SLT users to have attempted to quit during the 12 months before the survey and to intend to quit in the future (aPR: 1.09, 95%CI: 1.02–1.16). The corresponding aPRs were even higher for dual users (smoked tobacco and used SLT). Future intention to quit for both smokers (aPR: 1.45, 95%CI: 1.38–1.53) and SLT users (aPR: 1.87, 95%CI: 1.76–1.98) was significantly associated with their past quitting attempts. Most of those who had attempted to quit did not receive any treatment. Proactive and tailored interventions to promote quitting and expansion of tobacco cessation methods are recommended.