bluuebunny/crossref_metadata_2025 huggingface dataset:
A geografia da alimentação no contexto da pandemia: repensando os sistemas alimentares para o sustento dos corpos e do planeta
Authors: PONTES PATRÍCIA
Published: December 2020

RESUMOA crise proveniente da pandemia do novo coronavírus vem tornando ainda mais urgente a necessidade de repensarmos práticas individuais e sociais e as formas como elas afetam o planeta. Uma dessas práticas diz respeito à nossa alimentação, cada vez mais dependente de um sistema alimentar industrial que aprofunda problemas socioeconômicos, ambientais e sanitários – como as pandemias. Aliando a análise de aspectos estruturais às dimensões culturais e interseccionais que influenciam os hábitos alimentares contemporâneos, visamos com este artigo refletir sobre os impactos das nossas escolhas alimentares sobre o meio ambiente e os diferentes grupos populacionais, buscando uma geografia da alimentação que proponha/construa caminhos alternativos para a redução desses impactos e para a transformação dos atuais sistemas alimentares.Palavras-chave: Geografia da alimentação. Pandemia. Sistema alimentar. Meio ambiente. Grupos populacionais. The geography of food in the context of the pandemic: rethinking food systems to sustain bodies and the planetABSTRACTThe crisis due to the pandemic of the new coronavirus is making the need to rethink individual and social practices and the ways they affect the planet even more urgent. One of these practices concerns our food, which is increasingly dependent on an industrial food system that deepens socioeconomic, environmental and health problems – such as pandemics. Combining the analysis of structural aspects with the cultural and intersectional dimensions that influence contemporary eating habits, with this article we aim to reflect on the impacts of our food choices on the environment and different population groups, seeking a geography of food that proposes / builds alternative paths to reduce these impacts and transform current food systems.Keywords: Geography of food. Pandemic. Food system. Environment. Population groups.
As implicações da covid-19 na vida “Social”: crise política e desarranjo socioinstitucional
Authors: ASSAI JOSÉ HENRIQUE SOUSA
Published: December 2020

RESUMOA crise epidemiológica da covid-19 demonstrou a ineficiência de um plano governamental acerca da saúde pública no Brasil; mais do que isso, ela trouxe consigo um corolário remissivo de uma crise social sem precedentes. Perante o cenário pandêmico da covid-19 e levando em consideração os ancoramentos teórico-metodológicos da teoria crítica e da filosofia social, quero explicitar nesta pesquisa que essa crise social não pode ser debitada unicamente na conta da covid-19, porém ao desarranjo social que ela evidenciou. Daí que, a meu ver, esse estado de crise social decorre da não-efetivação da esfera do “Social” entendido basicamente pelas relações, instituições e práticas sociais (1). Tomarei como referências um dual quadro categorial: (2) a crise nas práticas e relações sociais mediante uma debilitada compreensão do que seja ideologia (quadro político) e a crise institucional entre os poderes (3).Palavras-chave: Filosofia Social. Teoria Crítica. Covid-19. Patologia Social.The implications of covid-19 to “Social” life: political crisis and social-institutional derangementABSTRACT The epidemiological crisis from covid-19 demonstrated the inefficiency of a governmental plan on public health in Brazil; moreover, it brought with it a corollary which refer to an unprecedented social crisis. In front of pandemic scenario of covid-19 and taking into account the theoretical-methodological assumptions of critical theory and social philosophy, I want to make explain in this research that this social crisis can’t be debited just because of covid-19, but to the social derangement which it highlighted. From my the point of view this social crisis derive from the not-effectuation of the “Social” sphere basically understood by social relations, institutions and practices (1). As of this issue, I will take as reference a dual categorical framework: (2) the crisis in social practices and relations through a weakened understanding with regard to ideology (political framework) and the institutional crisis between the public powers in our Republic State (3). Keywords: Social Philosophy. Critical Theory. Covid-19. Social Pathology.
Reprezentacje architektury w filmach science fiction jako symboliczny wyraz wizji przyszłości
Authors: Wąs Cezary
Published: December 2020

W filmach gatunku science fiction z II poł. XX i początku XXI w. zawartych zostało wiele wizji przyszłości, które były jednocześnie refleksją nad osiągnięciami i mankamentami współczesności. W latach 60. XX w. w dziełach kinematografii dominował optymizm i wiara w możliwość nigdy nie kończącego się postępu. Przewidywano zanik podziałów politycznych między blokami państw i wspólną eksplorację kosmosu. Scenografowie podejmowali współpracę z naukowcami, która przejawiała się w ukazywaniu kosmicznych konstrukcji dalece przewyższających realne możliwości techniczne. Poczynając od lat 70. XX w. w filmach zaczął narastać pesymizm i przekonanie, że przyszłość przyniesie przede wszystkim nasilenie negatywnych zjawisk teraźniejszości. Obawy przed przyszłością nasycone były wskazaniem na różne możliwe defekty i nierozwiązywalne sprzeczności między nimi. Kiedy zatem pewna część dystopijnych wizji obrazowała zagrożenie wzrostem przestępczości, to inna przedstawia przyszłość jako nasyconą mechanizmami kontroli państwowej i powszechnością inwigilacji. Pokazywane na ekranach lęki wzbudzał także przez rozrost wielkich korporacji, zwłaszcza zaś zyskiwanie przez nie wpływów politycznych czy też pozostawanie poza systemem demokracji. Twórcy filmów przedstawiali też swoje przypuszczenia związane z tworzeniem przez korporacje nowych rodzajów broni, których stosowanie przekraczało aktualne normy prawne. Szczególne zastrzeżenia dotyczyły badań nad bronią biologiczną i możliwością rozprzestrzenienia się śmiercionośnych wirusów. Trwogę wzbudził ponadto rozwój robotyki i badań nad sztuczną inteligencją, której skutkiem musiało być pojawienie się androidów i nieuchronnych napięć w ich relacjach z ludźmi. Osobnym problemem stały się dla filmowców hybrydy będące połączeniem ludzi i części elektronicznych. Podobnie zastanawiał scenarzystów i reżyserów rozwój inżynierii genetycznej, który prowadził do tworzenia zmutowanych osobników ludzkich. Pewna część filmowych dystopii rozważała możliwość upadku systemów demokratycznych i rozwoju w ich miejsce reżimów autorytarnych, często w oparciu o szerokie poparcie społeczne. W obrębie tej odmiany dystopii mieszczą się także filmy przestawiające konsekwencje współczesnego hedonizmu i konsumpcjonizmu. Problemem jest jednak, iż dzieła krytyczne wobec wymienionych zjawisk same bywały reklamami atrakcyjnych produktów.
Desinformação, infodemia e caos social: impactos negativos das fake news no cenário da COVID-19
Authors: Ferreira João Rodrigo Santos , Lima Paulo Ricardo Silva , Souza Edivanio Duarte de
Published: December 2020

Os meios digitais promovem a disseminação de informação em grande escala, inclusive fake news, gerando desinformação, infodemia e caos social. Este artigo buscou evidenciar a correlação entre essas temáticas, tomando como exemplo o cenário caótico marcado pela pandemia da COVID-19. Trata-se de ensaio de caráter bibliográfico e documental com abordagem qualitativa. Identifica impactos negativos no cenário da pandemia promovidos por informações imprecisas e inverídicas. Considera-se que a competência crítica em informação e a atuação das agências de fact-checking se destacam como elementos indispensáveis para lidar com os danos provenientes da combinação desses elementos.
Classificação dos laboratórios de pesquisa biomédica baseada em publicações científicas: o caso do Instituto Oswaldo Cruz
Authors: Sampaio Ricardo Barros , Martins Vitor Hugo da Silva , Christiaan Marie Noyons Everard , Sacerdote Helena Célia de Souza
Published: December 2020

Dada a importância do Instituto Oswaldo Cruz para as pesquisas biomédicas, em nível nacional e internacional, que visam ao desenvolvimento tecnológico, à inovação e ao fornecimento de serviços de referência para o diagnóstico de doenças infecciosas e genéticas e para o controle vetorial, um mapeamento da produtividade dos seus laboratórios pode contribuir para o planejamento dos recursos, considerando-se as necessidades e as prioridades das demandas de saúde regionais. O problema de pesquisa considerou o relato dos gestores a respeito da divisão dos laboratórios não se mostrar eficiente para atender às necessidades de investimento em pesquisas de saúde, devido à grande pulverização de recursos. O objetivo foi avaliar e apresentar as potencialidades de colaboração, de interação e de integração entre os laboratórios de pesquisa, considerando as publicações científicas dos seus pesquisadores, as áreas de pesquisa e as palavras-chave relacionadas aos laboratórios, também as áreas de conhecimento e os medical subject headings encontrados nas bases ISI Web of Science e PubMed, respectivamente. A metodologia utilizada compreendeu a análise de redes quanto à colaboração e à classificação científica das publicações; e a análise de textos instrumentalizada pelo software IRAMuTeQ. Como conclusão, foram apresentadas possibilidades de agrupamentos com diferentes abordagens. No entanto, esclarece-se que foram utilizadas apenas informações acerca de publicações científicas. Isso pode implicar um viés nos resultados encontrados, havendo, portanto, necessidade de um parecer de especialistas acerca desses resultados. A sugestão é que a pesquisa seja aprofundada, considerando os programas de pós-graduação do Instituto Oswaldo Cruz e de seus professores, além de trabalhos de dissertação e de tese defendidos nos últimos anos (como o E-lattes) e que os seus resultados sejam apresentados em outras publicações.
Caracterizando o processo de doutoramento no Brasil ao longo dos anos: período de formação, sexo e produção acadêmica
Authors: Digiampietri Luciano Antonio , Tuesta Esteban Fernandez , Köhler André Fontan , Delgado Karina Valdivia , Bernardes Júnior João Luiz
Published: December 2020

O estudo da produção científica e da formação acadêmica em um país, área ou mesmo instituição é uma das principais funções da cientometria; por exemplo, ao conseguir apontar quais instituições têm fornecido mais alto retorno à sociedade. Este artigo analisa o universo de indivíduos que, de 1970 a 2016, concluíram ou orientaram um doutorado, com base em dados extraídos de currículos cadastrados na Plataforma Lattes; esse conjunto totaliza 174.318 indivíduos, a partir do qual se traça um amplo panorama do processo de doutoramento no Brasil. Especial atenção é dedicada ao sexo dos orientados e orientadores, inclusive as quatro configurações possíveis de ocorrer entre eles, bem como às diferenças existentes entre as nove grandes áreas de atuação. Além do processo de doutoramento em si, analisa-se a produção científica dos orientados, com e sem parceria com o orientador, o tempo de ligação entre eles, e a parcela de orientados que permanece no meio acadêmico. Os resultados mostram crescente participação feminina, com a porcentagem de orientadas tendo ultrapassado a de orientados, em 2000, e a de orientadoras aparentando seguir a mesma tendência, mas com 20 anos de atraso. Mostram também um crescimento na produção de artigos em periódicos e uma queda na porcentagem de orientados que segue carreira acadêmica, aspecto em que o ano de conclusão do doutorado mostrou-se o fator com mais alta influência.
Discurso LGBTfóbico no ciberespaço do sertão pernambucano: discriminação e resistência
Authors: Silva Danuzio Weliton Gomes da , Castro Gustavo Henrique Carvalho de , Siqueira Marcus Vinicius Soares
Published: December 2020

Este estudo discute como as questões LGBT têm sido alvo de comentários em blogs, suscitando discursos opressores ao mesmo tempo em que favorecem contradiscursos de resistência que dão visibilidade positiva às dissidências de gênero e de sexualidade. Diante desse paradoxo, o objetivo foi investigar, em dois blogs do sertão pernambucano, interações virtuais que confirmam essas relações de força. Empregando a análise foucaultiana do discurso, verificaram-se, principalmente, a influência do discurso de moral cristã, conjugado ao da moral biológica, e a valorização de práticas discursivas que assujeitam os indivíduos à heterossexualidade compulsória e que privilegiam a biologia em detrimento de questões culturais e sociais. De outra parte, o exercício da resistência demonstrou comportamentos menos hipócritas e defendeu a inclusão da inteligibilidade LGBT nessas ordens discursivas. Conclui-se que os blogs são dispositivos em que os discursos passam a ser cada vez mais instrumentalizados conforme os interesses. Ademais, estimula-se o debate sobre a importância dos comentários na perpetuação de valores conservadores e suas repercussões sociais e políticas.
Tendências de inovação em serviços de bibliotecas universitárias: estudo de caso do CRAI Universitat Pompeu Fabra em Barcelona, Espanha
Authors: Corrêa Elisa Cristina Delfini , García-Quismodo Miguel Ángel Marzal
Published: December 2020

Neste artigo, são analisados produtos e serviços de base digital oferecidos pelo Centro de Recursos para Aprendizagem e Investigação da Universitat Pompeu Fabra em Barcelona, Espanha. O perfil da pesquisa é comparativo, bibliográfico e documental, e os dados foram coletados a partir de leitura e análise dos relatórios do New Media Consortium – NMC Horizon Report: 2015 Library Edition e NMC Horizon Report: 2017 Library Edition e da página web do Centro de Recursos para Aprendizagem e Investigação da Universitat Pompeu Fabra. Dados foram coletados também por meio de observação não participante realizada em visita à biblioteca do Campus del Poblenau, o que permite caracterizar a pesquisa como estudo de caso. Os dados foram submetidos a análise de conteúdo e foram categorizados. Os resultados demonstram a presença de muitas das tendências apresentadas nos relatórios do New Media Consortium, em especial aquelas de uso de tecnologia mais básica. É possível também observar, um pouco mais timidamente, a personalização de alguns serviços de acordo com as necessidades dos pesquisadores, assim como a oferta de ferramentas 2.0, bem como a entrega de conteúdos via dispositivos eletrônicos. O uso de tecnologias mais avançadas como Big Data, inteligência artificial ou tecnologia de localização, por exemplo, ainda não é encontrado. Conclui-se que são perceptíveis esforços para uma constante implementação de serviços e produtos de base digital, e, embora avanços tecnológicos mais sofisticados ainda não sejam encontrados, é possível identificar uma aproximação com as tendências apontadas pelos relatórios analisados.
Análise da paisagem cafeeira representada: visões interdisciplinares
Authors: Espírito Santo Silvia Maria do
Published: December 2020

O presente artigo opera a leitura do documento a partir dos elementos apriorísticos da análise da imagem da paisagem, frente à experiência empírica, subsidiária da observação do objeto documental selecionado. Neste estudo, apresentam-se três objetivos: a forma de coleta de informações dos documentos relativos a acervos históricos, a análise dos contextos culturais e  os argumentos que apontam para uma reflexão da articulação interdisciplinar entre áreas das humanidades. Para esta pesquisa, aplica-se o método quadripolar no estudo dos contextos culturais, entre os polos morfológico, prático, epistemológico e teórico. Conclui-se que, ao enfatizar a articulação e elementos advindos da “dinâmica investigativa quadripolar”, permite-se problematizar a leitura linear da paisagem na temática do Café para novas abordagens científicas e culturais.
Imaginário, memória e arquétipos
Authors: Fialho Francisco Antonio Ferreira , Bezerra Denise Maria
Published: December 2020

O artigo explora a ideia do imaginário como cultura e apresenta os pensamentos da Escola de Grenoble como uma nova forma de se fazer ciência. As hermenêuticas indo além das fenomenologias. O mito como base para as narrativas levando a uma mitanálise. Os arquétipos por detrás dos mitos. Explora-se enfim o conceito de bacia semântica como uma das formas de se produzir conhecimento
A escansão do olhar: Antonio Carlos Santos e o método
Authors: Giorgi Artur De Vargas
Published: December 2020

A escansão é um dos gestos fundamentais da crítica e da criação. Seu exercício coincide com o apuro da descontinuidade, ou seja, com a sensibilidade para as diferenças. Antonio Carlos Santos faz desse gesto um princípio operatório. Assim, entre a crítica e a criação, elabora o diferimento como um método, com o qual emerge o fundamento ausente que nos une, distendido nas passagens entre nomes, tempos, espaços, afetos.
La trata de seres humanos en España. Análisis crítico de la normativa española y propuestas para una mayor protección de la víctima
Authors: Alonso García Serena
Published: December 2020

Este trabajo tiene por objeto exponer la problemática de la trata de seres humanos en España, mostrando cómo algunos de los elementos que están presentes en la normativa y los planes de actuación, no resultan adecuados a la hora de afrontar este fenómeno desde una perspectiva de respeto a los derechos humanos de las víctimas. Para ello, tras la definición del concepto y su diferenciación con figuras afines, se procederá a hacer un repaso por las exigencias que derivan de la legislación internacional, determinando posteriormente el grado de adecuación del ordenamiento español a las mismas, así como las carencias detectadas. A continuación, se analizará el mecanismo de identificación de las víctimas tal y como está estipulado en España, resaltando sus posibles efectos adversos en relación con los procesos de victimización secundaria. Por último, y sobre la base del estudio comparado de los ordenamientos de siete países europeos, se procede a hacer una serie de recomendaciones que podrían suponer una mejora de la situación de las víctimas, al tiempo que acercarían al Estado español a sus compromisos internacionales en esta materia.
Численное исследование двухфазного потока в центробежном пылеуловителе на основе двухжидкостной модели турбулентности
Authors: Маликов Зафар Маматкулович , Malikov Zafar Mamatkulovich , Назаров Фаррух Холиeрович , Nazarov Farrux Xoliyorovich
Published: December 2020

Известно, что математическое моделирование закрученных турбулентных потоков
является сложной проблемой. Исследование таких потоков с помощью методов
прямого моделирования (DNS) или моделями больших вихрей (LES) требуют больших вычислительных ресурсов. А численное исследование двухфазного турбулентного потока внутри центробежного пылеуловителя на основе упомянутых методов
на сегодняшний день практически не представляется возможным. Поэтому для исследования таких потоков приемлемыми математическими моделями являются модели турбулентности, основанные на замыкании уравнений Навье-Стокса, осредненных по Рейнольдсу (RANS). Однако линейные модели RANS, в основе которых
лежит гипотеза Буссинеска, не пригодны для решения подобных задач. Дело в том,
что гипотеза Буссинеска предполагает изотропную турбулентность, а в случае вращающихся течений возникает анизотропная турбулентность. При небольших закрутках потока в линейные модели RANS вводятся специальные поправки. При
сильных закрутках потока, например, как в центробежных пылеуловителях, эти
поправки могут быть недостаточными для получения приемлемых численных решений. Поэтому в таких случаях рекомендуется использовать нелинейные RANS
модели, например, на основе рейнольдсовых напряжений. Но эти модели очень
сложны и громоздки для исследования двухфазных сред. В последнее время появилась новая двухжидкостная модель турбулентности. Данная модель имеет высокую
точность и проста в реализации при решении практических задач. Поэтому целью
настоящей работы является численное исследование двухфазного турбулентного
потока внутри центробежного пылеуловителя на основе новой двухжидкостной
модели. Для верификации модели полученные численные результаты сопоставляются с экспериментальными данными. В работе также представлены результаты,
полученные по линейной модели SARC.
IDENTIFIKASI POLA ASUH ORANG TUA TUNGGAL PADA ANAK USIA DINI
Authors: Ulfah Amira Adlina , Fauziah Puji Yanti
Published: December 2020

Pola asuh adalah karakteristik yang dimiliki orang tua dalam pengasuhan pada anak yang dilakukan secara berkelanjutan. Ada tiga jenis pola asuh yang biasa diterapkan oleh orang tua yaitu demokratis, permisif dan otoriter (Baumrind, 2010). Penelitian ini bertujuan untuk mengetahui pola asuh yang diterapkan oleh orang tua tunggal pada anak usia dini. Penelitian ini merupakan penelitian deskriptif dengan menggunakan pendekatan kuantitatif dan teknik perekrutan partisipan secara purposive sampling. Kriteria perekrutan partisipan dalam penelitian ini adalah orangtua tunggal yang memiliki anak usia dini dan ada 60 partisipan, 9 laki-laki dan 51 perempuan, yang memenuhi kriteria tersebut. Data penelitian diperoleh melalui penyebaran kuesioner melalui google form kepada para partisipan tesebut selama enam bulan. Hasil penelitian ini menunjukkan bahwa ada 51,4% partisipan penelitian yang menerapkan pola asuh demokratis, 27,9% menerapkan pola asuh otoriter dan 20,7% menerapkan pola asuh permisif pada anak usia dini.

Parenting is a set of characteristic that parents have in caring for their children on a sustainable manner. There are three types of parenting styles that are usually applied by parents, namely democratic, permissive and authoritarian (Baumrind, 2010). This research aims to determine the parenting styles that applied by single parents in early childhood. This research is a descriptive study using a quantitative approach and purposive sampling technique. The inclusion criteria for participants recruitment was single parent who has early age children and there were 9 male and 51 female participants who met this criteria. Data were collected by distributing questionnaires to those participants for six months. The results of this study indicate that there are 51,4% of study participants who apply democratic parenting, 27.9% apply authoritarian parenting and 20.7% apply permissive parenting in early childhood.
Lubang Resapan Biopori untuk Meningkatkan Kapasitas Penyimpanan Air di Daerah Perakaran Jeruk Keprok (Citrus reticulata)
Authors: Aji Titistyas Gusti , Sutopo NFN , Palupi Norry Eka
Published: December 2020

Lubang Resapan Biopori (LRB) dan penambahan bahan organik ke dalam LRB dapat memperbaiki sifat fisik, kimia, dan biologi tanah pada jangka panjang. Tujuan penelitian ini adalah mempelajari pengaruh jumlah LRB dan jenis bahan organik pengisi LRB terhadap kapasitas penyimpanan air di daerah perakaran tanaman jeruk keprok dewasa di lahan kering dan pengaruhnya terhadap kualitas buah. Penelitian disusun berdasarkan Rancangan Acak Kelompok dengan faktor yang diujikan adalah kombinasi jumlah LRB dan bahan pengisi LRB. Hasil percobaan menunjukkan bahwa perlakuan jumlah LRB, baik 4, 5, maupun 6 lubang dan bahan pengisi baik cocopeat maupun zeolit meningkatkan lengas tanah di daerah perakaran tanaman jeruk pada bulan kering. Penambahan cocopeat, zeolit, atau pupuk kandang ke dalam LRB meningkatkan kandungan jus. Perlakuan bahan pengisi serasah pada LRB dan tanpa LRB meningkatkan kandungan padatan terlarut total (PTT), sedangkan penambahan zeolit dan serasah serta perlakuan tanpa LRB meningkatkan kandungan asam tertitrasi total (ATT). Kadar air pada daerah perakaran yang tinggi menyebabkan peningkatan kandungan jus, serta penurunan kandungan PTT dan ATT pada buah jeruk keprok. Pembuatan LRB pada awal musim hujan dapat dilakukan sebagai upaya menabung air hujan sehingga dapat menghindarkan tanaman dari pengaruh negatif defisit air pada musim kering.

Keywords

Air; Buah jeruk keprok; Kualitas; Lubang resapan biopori; Rizosfer 

Abstract

Biopore Infiltration Holes (BIH) and addition of organic matters to the BIH can improve the physical, chemical, and biological properties of the soil. The purpose of this study was to study the effect of BIH and type of BIH filler on water storage capacity in the rhizosphere of mature mandarin plants on dry land and their effect on fruit quality. The study employed a randomized block design with the tested factors of combination of BIH number and BIH filler material. The results showed that either 4, 5 or 6 holes BIH and fillers of both cocopeat and zeolite increased the soil water content in the rhizosphere in the dry months. Adding cocopeat, zeolite, or manure into BIH increased the juice content. Weeds as fillers and treatment without BIH increased the total soluble solids (TSS), while addition of zeolite and weeds and treatment without BIH increased the total acid (TA). High water content in the rhizosphere caused an increase in juice content, but a decrease in TSS and TA. Making BIH at the beginning of rainy season is an effort to save rainwater so that it can prevent plants from the negative influence of water deficit in the dry season.
Miradas recientes sobre/desde el peronismo en el cine argentino de ficción
Authors: Dillon Alfredo
Published: December 2020

El artículo realiza un análisis formal y narrativo de dos películas biográficas recientes sobre el peronismo: Juan y Eva (Paula de Luque, 2011) y Puerta de Hierro. El exilio de Perón (Víctor Laplace y Dieguillo Fernández, 2012). Ambos films son leídos en relación con el clima de época que comparten: el del tercer gobierno kirchnerista en la Argentina. Construidas a partir de dos estéticas narrativas contrapuestas –una post-épica minimalista por un lado, una recuperación discursiva de la épica peronista por el otro–, las dos películas comparten, sin embargo, una mirada idealizadora sobre las figuras de Juan Domingo y Eva Perón.
Repeated rearrangement of the GSL‐AOP locus contributed to spatio‐temporal variation in defense chemistry in Arabidopsis
Authors: Weber Konrad , Krych Lucasz , Burow Meike
Published: December 2020

Plants coordinate metabolic and developmental processes with the help of genetically variable, interconnected regulatory networks. The GSL-AOP locus in Arabidopsis thaliana encodes enzymes involved in the biosynthesis of glucosinolate defense compounds and has been attributed regulatory functions e.g. in flowering time control. To correlate genetic and phenotypic variation linked to GSL-AOP, we conducted a phylogenetic analysis across 1135 accessions and found that the available short-read sequencing data does not fully resolve the structural diversity in the locus. We analyzed a selection of 74 accessions for glucosinolate profiles and flowering time under different conditions and acquired long-read sequence information for glucosinolate and flowering time loci. Especially in the Caucasus region, structural variation in GSL-AOP was associated with conditional, tissue-specific glucosinolate profiles. Variation in FLC among the Caucasian accessions correlated with variation in the flowering time response to vernalization, suggesting that local adaptation has shaped defense and development in an orchestrated manner.
Tea flavonoids blocking multiple SARS-CoV-2 protein targets judged from molecular docking
Authors: Wang Lufei , Sang Siyao , Su Mingjie , Wang Simin , Li Hui
Published: December 2020

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic. Flavonoids derived Chinese patent medicines has outstanding curative effects for the improvement and treatment of COVID-19. There are numerous studies suggesting that flavonoids-rich tea have antiviral effects. However, bioactive compounds from tea flavonoids with anti-COVID-19 effect, and the potential molecular mechanisms are unclear. In this study, we performed a molecular docking of 468 tea flavonoids and its derivatives with main protease (Mpro), angiotensin-converting enzyme 2 (ACE2), RNA dependent RNA polymerase (RdRp), compared with the positive control drugs of each target. The results suggested that ACE2 and RdRp are the main targets inhibited by tea flavonoids.Q3G Isovitexin, and TF would be considered as the potential candidate compounds of RdRp and ACE2. Our study provides a theoretical basis for further drug design of anti-COVID-19.
Plasma Activated Medium Induces Apoptosis in Chemotherapy-resistant Ovarian Cancer Cells: High Selectivity and Synergy with Carboplatin
Authors: Rasouli Milad , Mehdian Hassan , Hajisharifi Kamal , Amini Elaheh , Ostrikov Kostya , Robert Eric
Published: December 2020

Recently, cold atmospheric pressure plasmas (CAP) have emerged as a promising oncotherapeutic modality, through physical and chemical effects. Here, for the first time, A2780 CP and SKOV-3 cells, relevant to ovarian cancer and GCs as normal ovarian cells were evaluated through CAP directly, indirectly, and concomitant modality of plasma activated medium (PAM) with common drugs to overcome chemotherapy resistance in ovarian cancer. Our results confirm the high potential and the stronger selectivity of PAM in comparison to CAP for the selected cell lines and selectivity mechanism was related to the pH and concentration of H2O2, NO2-, and NO3- reactive species in the plasma stimulated medium. Compared to the combination of common carboplatin (CAR) and paclitaxel (PTX) chemotherapy treatments, the PAM-based treatment is very promising for ovarian cancer treatment. Our data verify that PAM alone and in combination with carboplatin sensitizes cancer cells to carboplatin, inhibits the SOD1 gene, and selectively induces apoptosis accompanied with high expression of p53, Bax, and activation of Caspase-3. Collectively, our observations revealed PAM treatment is an innovative and emerging technology for the future combination or multimodal ovarian cancer oncotherapy.
Solo Surgery in Single Incision Laparoscopic Myomectomy
Authors: Chung Do Young
Published: December 2020

Background The intracorporeal suture during single incision laparoscopic surgery is challengeable. Since laparoscopic myomectomy requires not only one assistant to hold a camera but also other to hold the uterine manipulator, it is difficult to perform solo surgery in single port laparoscopic myomectomy through intracorporeal suture.ResultsThe three patients underwent single incision laparoscopic myomectomy. The size of the largest myoma was 4.8-5.6cm. The operation was performed by the surgeon alone without assistants. The passive camera holder was positioned near the patient's body. Without using uterine manipulator, the uterus was hung from the abdominal wall using a thread. The uterine myoma was enucleated, which was removed from the outside with the umbilicus incision site. The uterine wound was double sutured to avoid any uterine defects. The outer suture of wound was sutured by a method such as simple continuous suture, Lembert suture, or baseball suture. The operation time was 95-105 minutes.There were no specific complications associated with the surgery. The patients were discharged on the second day after surgery.ConclusionsSolo surgery in single incision laparoscopic myomectomy using camera holder was successfully performed without using a uterine manipulator.
SNHG3 cooperates with ELAVL2 to modulate cell apoptosis and extracellular matrix accumulation by stabilizing SNAI2 in human trabecular meshwork cells under oxidative stress
Authors: Li Sizhen , Yang Qingsong , Zhou Zixiu , Fu Min , Yang Xiaodong , Hao Kuanxiao , Liu Yating
Published: December 2020

Background: Glaucoma is the main reason for irreversible blindness, and pathological increased intraocular pressure is the leading risk factor for glaucoma. It is reported that trabecular meshwork cell injury is closely associated with the elevated intraocular pressure. The current study aimed to investigate the role of SNHG3 in human trabecular meshwork (HTM) cells under oxidative stress. Methods: A series of experiments including real-time quantitative polymerase chain reaction (RT-qPCR), subcellular fractionation assay, western blot analysis, cell counting kit-8 (CCK-8) assay, RNA pull down, flow cytometry analysis, and RIP assay were employed to explore the biological function and regulatory mechanism of SNHG3 in HTM cells under oxidative stress.Results: First, we observed that H2O2 induced SNHG3 upregulation in HTM cells. Then, we found that SNHG3 silencing alleviated H2O2-induced oxidative damage in HTM cells. Moreover, SNAI2 knockdown alleviated the oxidative damage induced by H2O2 in HTM cells. Mechanistically, SNHG3 bound with ELAVL2 to stabilize SNAI2. Finally, SNAI2 overexpression counteracted the effect of SNHG3 silencing on H2O2-induced HTM cells. Conclusion: Our results demonstrated that SNHG3 cooperated with ELAVL2 to modulate cell apoptosis and extracellular matrix (ECM) accumulation by stabilizing SNAI2 in HTM cells under oxidative stress.
Changes in Properties of Tar Obtained During Underground Coal Gasification Process
Authors: Wiatowski Marian , Muzyka Roksana , Kapusta Krzysztof , Chrubasik Maciej
Published: December 2020

In this study, the composition of tars collected during a six-day underground coal gasification (UCG) test at the experimental mine ‘Barbara’ in Poland in 2013 was examined. During the test, tar samples were taken every day from the liquid product separator and analysed by the methods used for testing properties of typical coke oven (coal) tar. The obtained results were compared with each other and with the data for coal tar. As gasification progressed, a decreasing trend in the water content and an increasing trend in the ash content were observed. The tars tested were characterized by large changes in the residue after coking and content of parts insoluble in toluene and by smaller fluctuations in the content of parts insoluble in quinoline. All tested samples were characterized by very high distillation losses, while for samples starting from the third day of gasification, a clear decrease in losses was visible. A chromatographic analysis showed that there were no major differences in composition between the tested tars and that none of the tar had a dominant component such as naphthalene in coal tar. The content of polycyclic aromatic hydrocarbons (PAHs) in UCG tars is several times lower than that in coal tar. No light monoaromatic hydrocarbons (benzene, toluene, ethylbenzene and xylenes - BTEX) were found in the analysed tars, which results from the fact that these compounds, due to their high volatility, did not separate from the process gas in the liquid product separator.
UMOD Polymorphisms Associated With Kidney Function, Serum Uromodulin and Risk of Mortality Among Patients With Chronic Kidney Disease, Results From the C-STRIDE study
Authors: Wang Jinwei , Liu Lili , He Kevin , Gao Bixia , Wang Fang , Zhao Ming-Hui , Zhang Luxia
Published: December 2020

Background: Several genome-wide association studies have identified single nucleotide polymorphisms (SNPs) in uromodulin (UMOD) gene, such as 12917707, rs4293393, rs6497476 and rs13333226, as susceptibility genetic variants associated with chronic kidney disease (CKD) based on the case-control study design. We aimed to explore the associations of the variants with baseline phenotypes and prospective prognosis of CKD among 2731 Chinese patients with CKD stage 1-4. Polymorphisms of rs11864909, rs4293393, rs6497476 and rs13333226 were genotyped using the Sequenom MassARRAY iPLEX platform.Results: rs13333226 and rs4293393 were in complete linkage disequilibrium. Based on the T dominant model, T allele of rs11864909 was associated with baseline levels of eGFR and serum uromodulin with linear regression coefficients of 2.68 (95% confidence interval [CI]: 0.61, 4.96) and -12.95(95%CI: -17.59, -7.98), respectively, after adjustment for cardiovascular and kidney specific risk factors. After a median follow-up of 4.94 years, both G allele of rs4293393/rs13333226 and C allele of rs6497476 were associated with reduced risk of all-cause mortality with multivariable adjusted hazard ratios of 0.341(95%CI: 0.105, 0.679) and 0.344(95%CI: 0.104, 0.671), respectively. However, no associations were found between the variants and eGFR slope in the linear mix effect model.Conclusions: Variant of rs11864909 in the UMOD gene was associated with levels of eGFR and serum uromodulin, while those of rs4293393 and rs6497476 associated with all-cause mortality among patients with CKD.
Maternal Inhalation of Humidifier Disinfectant Biocide Aerosol Particles, Polyhexamethylene Guanidine Phosphate, Induces Adverse Postnatal Development in Rat Offspring
Authors: Lee Jinsoo , Choi Seong-Jin , Jeong Ji-Seong , Kim Sang Yun , Lee Seung-Jin , Baek Sang-Ki , Lee Sang-Hyub , Kim Woojin , Cho Jae-Woo , Koh Eun Mi , Lee Kyuhong , Jeong Eun Ju , Nam Sang-Yoon , Yu Wook-Joon
Published: December 2020

Background: Inhalation exposure to polyhexamethylene guanidine phosphate (PHMG-P), one of the primary biocides used in humidifier disinfectants, caused a fatal pulmonary disease in Korea. Pregnant women were also exposed to PHMG-P, and subsequent studies showed that PHMG-P inhalation adversely affects their health and prenatal development. However, the postnatal developmental effects on prenatally PHMG-P-exposed offspring have not yet been investigated. Therefore, in this study, we examined the postnatal developmental effects of offspring with prenatally PHMG-P exposure. PHMG-P aerosol particles were exposed to pregnant rats via inhalation during pregnancy at concentrations of 0, 0.14, 1.60, and 3.20 mg/m3, and general and reproductive functions of pregnant rats were also investigated. After parturition, comprehensive postnatal development of offspring was investigated, including general systemic health, reproduction, and development. Results: At 3.20 mg/m3, increased perinatal death and decreased viability index (postnatal survival of offspring between birth and postnatal day 4) were observed. In addition, offspring had lower body weight at birth that persisted throughout the study. PHMG-P-exposed pregnant rats had severe systemic toxicities and increased gestation length. At 1.60 mg/m3, a decreased viability index was also observed with systemic toxicities of PHMG-P-exposed pregnant rats. Conclusion: As a result, maternal inhalation exposure of PHMG-P aerosol particles during pregnancy induced adverse postnatal development in rat offspring and they were observed with systemic toxicities of their maternal animals. The results of this study could be used for risk assessment of postnatal development of prenatally PHMG-P-exposed offspring as well as reproductive and developmental effects on females with PHMG-P inhalation exposure during pregnancy.
Stereotactic Lung Reirradiation for Local Relapse: A Case Series
Authors: Kinj Rémy , Casutt Alessio , Bennassi Alexander , Bouchaab Hasna , Vallet Véronique , Lovis Alban , Ozsahin Mahmut
Published: December 2020

Background: Local recurrence after lung SBRT for early stage NSCLC is rare but its treatment remains a challenge due to limited surgical options. We report a case series of 5 patients treated by stereotactic lung salvage reirradiation for local relapse after a previous lung SBRT.Material and methods: Included patients presented an isolated primary lung relapse within at least the 25% isodose of the previous SBRT treatment. Typical reirradiation schedule was 60 Gy in 8 fractions at isodose 80% and was delivered by Cyberknife® using Synchrony® fiducial tracking system. Dose summations were performed to evaluate the safety of the reirradiation. Results: We identified 5 patients presenting peripheral lesions. All reirradiated lesions were locally controlled after a median follow-up of 8.0 months (3,4-10,2). while PFS at 6 months was 60% (n = 3). We did not notice any Grade 3 or more acute or late adverse event.Conclusion: We observed encouraging short-term outcome of lung SBRT reirradiation in patients presenting isolated local relapse of an early-stage NSCLC. Further studies are necessary to confirm the safety and efficiency of this salvage treatment approach.
Functional and Patient-reported Changes in Swallowing and Voice After Combined Chemotherapy and Radiotherapy for Limited Stage Small Cell Lung Cancer
Authors: Frowen Jacqui , Gough Karla , Hughes Rhys , Drosdowsky Allison , Duffy Mary , Kiss Nicole , Phipps-Nelson Jo , Siva Shankar , Solomon Benjamin , Ball David
Published: December 2020

Purpose: To describe the nature and impact of dysphagia (difficulty swallowing) and dysphonia (impaired voice) in patients with limited-stage small cell lung cancer (SCLC) before and after chemoradiation.Methods: A prospective cohort study was conducted on patients receiving chemoradiotherapy for limited-stage SCLC. Patients received either 40Gy in 15 fractions, 45Gy in 30 fractions (delivered BD) or 50Gy in 25 fractions, commencing the second cycle of carboplatin/etoposide or cisplatin/etoposide chemotherapy. Outcomes included: videofluoroscopy swallowing studies (VFSS) to investigate aspiration, swallowing function, and oesophageal motility disorders; limitations to oral intake; patient-reported swallowing problems; and patient-reported voice problems. Data were collected before treatment and at one, three and six months post-treatment.Results: Twelve patients were enrolled. The oropharyngeal swallow was safe and functional at all time points. Three patients exhibited oesophageal motility disorders before treatment, while a further three exhibited disorders post-treatment. Oral intake was most compromised one month post-treatment with five patients being either tube dependent or eating only very limited diets. At all other times patients were eating a normal or near-normal diet. Despite normal oropharyngeal swallowing on VFSS, three patients reported moderate or severe swallowing difficulties one month post-treatment. Three additional patients reported moderate or severe difficulties three and six months post-treatment. Patients who reported swallowing difficulties one month post-treatment had all received a mean and maximum radiation dose to the oesophagus of ≥15.7Gy and ≥42Gy respectively. Dose-response relationships were no longer apparent three and six-months post treatment. Voice problems were variable, with the worst scores reported one month post-treatment.Conclusions: Although patient numbers are small, this study identified discordance between observed swallowing function and patient-reported problems, which has clinical implications for the management of patients with SCLC, as well as identifying areas for future research. Ongoing efforts to reduce mucosal toxicity in lung cancer patients are essential.
Effects of Robot-Aided Rehabilitation on Improving Ankle and Balance Performance of Stroke Survivors: A Randomized Controlled Trial
Authors: Zhai Xiaoxue , Wu Qiong , Li Xin , Xu Quan , Zhang Yanlin , Fan Senchao , Zhang Li-Qun , Pan Yu
Published: December 2020

Background: Stroke survivors with impaired ankle control due to stiff plantar flexors often experience abnormal posture control, which affects balance and locomotion. Forceful and safe stretching under intelligent control may decrease ankle stiffness and improve balance. The purpose of this study was to investigate the effects of robot-aided ankle rehabilitation on stroke survivors with ankle spasticity and the correlations between biomechanical properties and balance in these participants. Methods: Twenty patients poststroke with ankle spasticity received 20 minutes of stretching treatment daily over 2 weeks. The study group used a robot-aided ankle rehabilitation and the control group received manual stretching. Outcome measures included biomechanical, clinical evaluations, and the Pro-Kin balance test.Results: The study group significantly improved in joint stiffness and range of motion of dorsiflexion, Modified Ashworth Scale (MAS), trajectory lengths, elliptical trajectory, standard deviation medial/lateral, average speed forward/backward with eyes closed, and standard deviation forward/backward with eyes open in the Pro-Kin test (P<.05), but no significant changes were found in the control group in above indexes; significant decreases were found in the control group in trajectory length with eyes open (P<.05); dorsiflexion stiffness was positively correlated with the Pro-Kin test outcomes with eyes open and the MAS; no between-group significant changes were found except the activities of daily living after training (P<.05).Conclusions: Ankle stiffness may affect balance poststroke significantly. The robot-aided ankle rehabilitation improved biomechanical properties of the spastic ankle including stiffness, and it may help improve balance post-stroke.Trial registration: www.chictr.org.cn ChiCTR2000030108. Registered 23 February 2020. Retrospectively registered.
Single cell proteomics profiling reveals that embryo-secreted TNF-α plays a critical role during embryo implantation to the endometrium
Authors: Jiao Lv , Shan Xudong , Yang Haoxuan , Wen Yuting , Zhang Xueguang , Chen Hanxiao , Li Hengli , Tian Dongmei , Wang Chi Chiu , Zhang Ruizhe , Li Tin Chiu , Zhang Xiaohu , Zhao Xiaomiao , Lu Yao , Qin Lang , Zhu Minghui , Xu Wenming
Published: December 2020

Background

It has been long-known that endometrium-secreted cytokines play a critical role during embryo implantation. However, whether cytokines secreted from the embryo are relevant to the process of embryo implantation remains unclear.
Methods

The concentration of cytokines in embryo culture medium was tested using a newly developed, high-sensitivity single-cell proteomic platform and evaluated in comparison to embryo quality and clinical outcome. The effect of TNF-α on embryo and endometrium Ishikawa cells was investigated using immunofluorescence staining, CCK- 8 assay, TUNEL staining, and RT-qPCR.
Results

Of the 10 cytokines measured, only TNF-α concentration was significantly higher in the group with embryo implantation failure. Immunofluorescence staining showed that the expression of TNF-α was unevenly distributed in blastocysts, and the expression level was significantly correlated with the blastocyst inner cell mass (ICM) quality score. Gene profiling showed that addition of TNF-α led to increased expression of tumor necrosis factor receptor 1 (TNFR1) and apoptosis-related genes and that this could be inhibited by the TNF-α receptor inhibitor entanecept (ETA). In addition, an increased expression of water and ion channels, including AQP3, CFTR, ENaCA and CRISP2 was also observed which could also be inhibited by ETA.
Conclusions

Our results show that higher embryo-secreted TNF-α levels are associated with implantation failure through activation of TNF-α receptor, and TNF-α may be an independent predictor for pre-transfer assessment of the embryo development potential in IVF patients.