bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantifying Tree Diversity, Carbon Stocks, and Sequestration Potential for Diverse Land Uses in Northeast India
Authors: Sahoo Uttam Kumar , Tripathi Om Prakash , Nath Arun Jyoti , Deb Sourabh , Das Dhruba Jyoti , Gupta Asha , Devi N. Bijayalaxmi , Charturvedi Shiva Shankar , Singh Soibam Lanabir , Kumar Amit , Tiwari Brajesh Kumar
Published: October 2021

In the modern era, rapid anthropogenic activities in the vicinity of the Himalayas disturb the carbon sequestration potential resulting in climate change. For the first time, this study estimates the biomass and carbon storage potential of Northeast India’s diverse land uses through a biomass estimation model developed for this region. The mean tree density in tropical, subtropical, and temperate forests was 539, 554, and 638 trees ha−1, respectively. The mean vegetation carbon stock was the highest for temperate forests (122.09 Mg C ha−1), followed by subtropical plantations (115.45 Mg C ha−1), subtropical forests (106.01 Mg C ha−1), tropical forests (105.33 Mg C ha−1), tropical plantations (93.00 Mg C ha−1), and temperate plantations (50.10 Mg C ha−1). Among the forests, the mean soil organic carbon (SOC) stock up to 45 cm depth was the highest for tropical forests (72.54 Mg C ha−1), followed by temperate forests (63.4 Mg C ha−1) and subtropical forests (42.58 Mg C ha−1). A strong relationship between the tree basal area and biomass carbon storage was found for all land-use types. The land-use transformation from agriculture to agroforestry, and grassland to plantations increased both vegetation carbon (VC) and SOC stocks. The corresponding increase in VC and SOC was 40.80 and 43.34 Mg C ha−1, respectively, in the former, and 83.18 and 97.64 Mg C ha−1 in the latter. In general, the landscape-level estimates were drawn from site-level estimates in a given land-use type, and therefore, the corresponding values might be overestimated. Nevertheless, the results provide baseline information on carbon stock which may serve as a reference for devising appropriate land-use change policies in the region.
Mapping the Extent of Invasive Phragmites australis subsp. australis From Airborne Hyperspectral Imagery
Authors: Elmer Kathryn , Kalacska Margaret , Arroyo-Mora J. Pablo
Published: October 2021

Invasive species pose one of the greatest threats to global biodiversity. Early detection of invasive species is critical in order to prevent or manage their spread before they exceed the ability of land management groups to control them. Optical remote sensing has been established as a useful technology for the early detection and mapping of invasive vegetation populations. Through the use of airborne hyperspectral imagery (HSI), this study establishes a target detection methodology used to identify and map the invasive reed Phragmites australis subsp. australis within the entire extent of Îles-de-Boucherville National Park (Quebec, ON, Canada). We applied the Spectral Angle Mapper (SAM) target detection algorithm trained with a high accuracy GNSS ground truth data set to produce a park-wide map illustrating the extent of detected Phragmites. The total coverage of detected Phragmites was 26.74 ha (0.267 km2), which represents 3.28% of the total park area of 814 ha (8.14 km2). The inherent spatial uncertainty of the airborne HSI (∼2.25 m) was accounted for with uncertainty buffers, which, when included in the measurement of detected Phragmites, lead to a total area of 59.17 ha (0.591 km2), or 7.26% of the park. The overall accuracy of the Phragmites map was 84.28%, with a sensitivity of 76.32% and a specificity of 91.57%. Additionally, visual interpretation of the validation ground truth dataset was performed by 10 individuals, in order to compare their performance to that of the target detection algorithm. The overall accuracy of the visual interpretation was lower than the target detection (i.e., 69.18%, with a sensitivity of 59.21% and a specificity of 78.31%). Overall, this study is one of the first to utilize airborne HSI and target detection to map the extent of Phragmites over a moderately large extent. The uses and limitations of such an approach are established, and the methodology described here in detail could be adapted for future remote sensing studies of Phragmites or other vegetation species, native or invasive, at study sites around the world.
Meridional Temperature Difference Over Pan-East Asia and its Relationship With Precipitation in Century Scales
Authors: Sheng Bosi , Wang Haolin , Sun Wenbin , Li Haiyan , Chao Liya , Cheng Jiayi , Qian Gangzhen , Huang Boyin , Li Qingxiang
Published: October 2021

Based on the newly released China Merged Surface Temperature (China-MST) dataset, we diagnose the leading mode of covariation between surface temperature and the summer precipitation in Eastern China (EC). A possible connection between the surface temperature in East Asia and EC summer precipitation since the last century (1901–2016) is analyzed. Results indicate that there exists a remarkable meridional dipole pattern of surface temperature, defined as the meridional temperature difference index (MTDI). Meanwhile, there is a tripolar pattern of summer precipitation, which coincides with the first principal component of summer precipitation in EC. Further analyses show that when the MTDI is high in summer, an anomalous anticyclonic/cyclonic circulation is in East Asia’s mid/low latitude. The water vapor is down in the Yangtze and Huaihe River basins, and the ascending motion is weak, resulting in reduced precipitation over this region. At the same time, more water vapor and intensified ascending motion in North and South China tend to produce more summer precipitation. The opposite situations are found when the MTDI is low. Moreover, the MTDI closely relates with the Pacific Decadal Oscillation and the Atlantic Multidecadal Oscillation at interdecadal and multi-decadal scales. These results have been substantiated in the application of information flow analysis, a recently developed method in physics for the inference of causality between dynamical events.
Role of Export Diversification and Renewable Energy on the Load Capacity Factor in Indonesia: A Fourier Quantile Causality Approach
Authors: Fareed Zeeshan , Salem Sultan , Adebayo Tomiwa Sunday , Pata Ugur Korkut , Shahzad Farrukh
Published: October 2021

Sustainable development and reducing environmental pressure are major issues that concern developed as well as developing countries. Although researchers widely use carbon dioxide emissions and ecological footprint within the scope of environmental degradation, a more comprehensive ecological indicator is needed to assess environmental sustainability. In this context, the load capacity factor enables a comprehensive environmental sustainability assessment through the simultaneous analysis of biocapacity and ecological footprint. However, there are few studies analyzing the determinants of load capacity factor and this study aims to fill this gap for Indonesia. Using the recently developed Fourier quantile causality test, this study investigates the impact of income, export diversification, non-renewable and renewable energy consumption on the load capacity factor for Indonesia during 1965Q1–2014Q4. The results show unidirectional causality from non-renewable energy consumption to the load capacity factor at all quantiles, while income, export diversification, and renewable energy are the causes of environmental quality at middle and higher quantiles (within 0.5, 0.7, and 0.9). Most importantly, renewable energy and export diversification increase the load capacity factor and thus support environmental quality. In contrast, an increase in income and consumption of non-renewable energy reduces the load capacity factor. These results highlight the importance of renewable energy and export diversification for the sustainable development of Indonesia.
37 Years of Forest Monitoring in Switzerland: Drought Effects on Fagus sylvatica
Authors: Braun Sabine , Hopf Sven-Eric , Tresch Simon , Remund Jan , Schindler Christian
Published: October 2021

European beech is one of the most important deciduous tree species in natural forest ecosystems in Central Europe. Its dominance is now being questioned by the emerging drought damages due to the increased incidence of severe summer droughts. In Switzerland, Fagus sylvatica have been observed in the Intercantonal Forest Observation Program since 1984. The dataset presented here includes 179176 annual observations of beech trees on 102 plots during 37 years. The plots cover gradients in drought, nitrogen deposition, ozone, age, altitude, and soil chemistry. In dry regions of Switzerland, the dry and hot summer of 2018 caused a serious branch dieback, increased mortality in Fagus sylvatica and increased yellowing of leaves. Beech trees recovered less after 2018 than after the dry summer 2003 which had been similar in drought intensity except that the drought in 2018 started earlier in spring. Our data analyses suggest the importance of drought in subsequent years for crown transparency and mortality in beech. The drought in 2018 followed previous dry years of 2015 and 2017 which pre-weakened the trees. Our long-term data indicate that the drought from up to three previous years were significant predictors for both tree mortality and for the proportion of trees with serious (>60%) crown transparency. The delay in mortality after the weakening event suggests also the importance of weakness parasites. The staining of active vessels with safranine revealed that the cavitation caused by the low tree water potentials in 2018 persisted at least partially in 2019. Thus, the ability of the branches to conduct water was reduced and the branches dried out. Furthermore, photooxidation in light-exposed leaves has increased strongly since 2011. This phenomenon was related to low concentrations of foliar phosphorus (P) and hot temperatures before leaf harvest. The observed drought effects can be categorized as (i) hydraulic failure (branch dieback), (ii) energy starvation as a consequence of closed stomata and P deficiency (photooxidation) and (iii) infestation with weakness parasites (beech bark disease and root rots).
Comparative Cytogenetics Analysis Among Peckoltia Species (Siluriformes, Loricariidae): Insights on Karyotype Evolution and Biogeography in the Amazon Region
Authors: Santos da Silva Kevin , de Souza Augusto Cesar Paes , Pety Ananda Marques , Noronha Renata Coelho Rodrigues , Vicari Marcelo Ricardo , Pieczarka Julio Cesar , Nagamachi Cleusa Yoshiko
Published: October 2021

Peckoltia is widely distributed genus in the Amazon and Orinoco basins and the Guiana Shield, containing 18 valid species, and distinct morphotypes still needing description in the scientific literature due to its great taxonomic complexity. This study performed a comparative chromosomal analysis of two undescribed Peckoltia species (Peckoltia sp. 3 Jarumã and Peckoltia sp. 4 Caripetuba) from the Brazilian Amazon using conventional chromosome bands methods and in situ localization of the repetitive DNA (5S and 18S rRNA and U1 snRNA genes and telomeric sequences). Both species presented 2n = 52 but differed in their karyotype formula, probably due to inversions or translocations. The nucleolus organizer regions (NORs) showed distal location on a probably homeologous submetacentric pair in both species, besides an extra signal in a subtelocentric chromosome in Peckoltia sp. 4 Caripetuba. Heterochromatin occurred in large blocks, with different distributions in the species. The mapping of the 18S and 5S rDNA, and U1 snDNA showed differences in locations and number of sites. No interstitial telomeric sites were detected using the (TTAGGG)n probes. Despite 2n conservationism in Peckoltia species, the results showed variation in karyotype formulas, chromosomal bands, and locations of repetitive sites, demonstrating great chromosomal diversity. A proposal for Peckoltia karyotype evolution was inferred in this study based on the diversity of location and number of chromosomal markers analyzed. A comparative analysis with other Peckoltia karyotypes described in the literature, their biogeography patterns, and molecular phylogeny led to the hypothesis that the derived karyotype was raised in the left bank of the Amazon River.
2Vita-B Physical: An Intelligent Home Rehabilitation System Based on Microsoft Azure Kinect
Authors: Antico Mauro , Balletti Nicoletta , Ciccotelli Andrea , Ciccotelli Marco , Laudato Gennaro , Lazich Aldo , Notarantonio Marco , Oliveto Rocco , Ricciardi Stefano , Scalabrino Simone , Simeone Jonathan
Published: October 2021

Active rehabilitation is an exercise-based program designed to improve the level of function of people with motor disabilities. The effectiveness of such programs is strongly influenced by the correctness of the exercise execution. An exercise done incorrectly could even lead to a worsening of the health status. For this reason, specialists are required to guide the patient during the execution of an exercise. The drastic reduction of the costs of motion tracking systems has paved the way to the implementation of virtual assistant software able to automatically assess the correctness of an exercise. In this paper 2Vita-B Physical is presented, a rehabilitation software system properly designed to support both 1) the patients, by guiding them in the correct execution of an exercise; and 2) the physiotherapists, by allowing them to remotely check the progress of a patient. The motion capturing in 2Vita-B is performed by using the recently released Microsoft Kinect Azure DK. Thus, the system is easy to use and completely non-invasive. Besides the hardware and software requirements of the system, the results of a preliminary usability evaluation of the system conducted with 29 users is also reported. The results achieved are promising and provide evidence of the high usability of 2Vita-B Physical as home rehabilitation system.
PMEPA1 Is a Prognostic Biomarker That Correlates With Cell Malignancy and the Tumor Microenvironment in Bladder Cancer
Authors: Qiu Dongxu , Hu Jian , Hu Jiao , Yu Anze , Othmane Belaydi , He Tongchen , Ding Jian , Cheng Xu , Ren Wenbiao , Tan Xiyan , Yu Qiaoyan , Chen Jinbo , Zu Xiongbing
Published: October 2021

Prostate transmembrane protein androgen induced 1 (PMEPA1) has been reported to promote cancer progression, but the potential role of PMEPA1 in bladder cancer (BLCA) remains elusive. We assess the role of PMEPA1 in BLCA, via a publicly available database and in vitro study. PMEPA1 was identified from 107 differentially expressed genes (DEGs) to have prognostic value. GO, KEGG, and GSEA analysis indicated that PMEPA1 was involved in cancer progression and the tumor microenvironment (TME). Then bioinformatical analysis in TCGA, GEO, TIMER, and TISIDB show a positive correlation with the inflammation and infiltration levels of three tumor-infiltrating immune cells (TAMs, CAFs, and MDSCs) and immune/stromal scores in TME. Moreover, in vitro study revealed that PMEPA1 promotes bladder cancer cell malignancy. Immunohistochemistry and survival analysis shed light on PMEPA1 potential to be a novel biomarker in predicting tumor progression and prognosis. At last, we also analyzed the role of PMEPA1 in predicting the molecular subtype and the response to several treatment options in BLCA. We found that PMEPA1 may be a novel potential biomarker to predict the progression, prognosis, and molecular subtype of BLCA.
Plasma Gradient of Soluble Urokinase-Type Plasminogen Activator Receptor Is Linked to Pathogenic Plasma Proteome and Immune Transcriptome and Stratifies Outcomes in Severe COVID-19
Authors: Sarif Jafar , Raychaudhuri Deblina , D’Rozario Ranit , Bandopadhyay Purbita , Singh Praveen , Mehta Priyanka , Hoque Md. Asmaul , Sinha Bishnu Prasad , Kushwaha Manoj , Sahni Shweta , Devi Priti , Chattopadhyay Partha , Paul Shekhar Ranjan , Ray Yogiraj , Chaudhuri Kausik , Banerjee Sayantan , Majumdar Debajyoti , Saha Bibhuti , Sarkar Biswanath Sharma , Bhattacharya Prasun , Chatterjee Shilpak , Paul Sandip , Ghosh Pramit , Pandey Rajesh , Sengupta Shantanu , Ganguly Dipyaman
Published: October 2021

Disease caused by SARS-CoV-2 coronavirus (COVID-19) led to significant morbidity and mortality worldwide. A systemic hyper-inflammation characterizes severe COVID-19 disease, often associated with acute respiratory distress syndrome (ARDS). Blood biomarkers capable of risk stratification are of great importance in effective triage and critical care of severe COVID-19 patients. Flow cytometry and next-generation sequencing were done on peripheral blood cells and urokinase-type plasminogen activator receptor (suPAR), and cytokines were measured from and mass spectrometry-based proteomics was done on plasma samples from an Indian cohort of COVID-19 patients. Publicly available single-cell RNA sequencing data were analyzed for validation of primary data. Statistical analyses were performed to validate risk stratification. We report here higher plasma abundance of suPAR, expressed by an abnormally expanded myeloid cell population, in severe COVID-19 patients with ARDS. The plasma suPAR level was found to be linked to a characteristic plasma proteome, associated with coagulation disorders and complement activation. Receiver operator characteristic curve analysis to predict mortality identified a cutoff value of suPAR at 1,996.809 pg/ml (odds ratio: 2.9286, 95% confidence interval 1.0427–8.2257). Lower-than-cutoff suPAR levels were associated with a differential expression of the immune transcriptome as well as favorable clinical outcomes, in terms of both survival benefit (hazard ratio: 0.3615, 95% confidence interval 0.1433–0.912) and faster disease remission in our patient cohort. Thus, we identified suPAR as a key pathogenic circulating molecule linking systemic hyperinflammation to the hypercoagulable state and stratifying clinical outcomes in severe COVID-19 patients with ARDS.
Safety of Influenza A H1N1pdm09 Vaccines: An Overview of Systematic Reviews
Authors: Juvet Lene Kristine , Robertson Anna Hayman , Laake Ida , Mjaaland Siri , Trogstad Lill
Published: October 2021

Background

In 2009, a new influenza A H1N1 virus emerged causing a global pandemic. A range of monovalent influenza A H1N1pdm09 vaccines with or without adjuvants were developed. After the mass vaccination campaigns safety concerns related to H1N1pdm09 vaccines were reported. More than a decade later, reported AEFIs are still under scrutiny. We performed a systematic review aiming to synthesize the evidence on the safety of the H1N1pdm09 vaccines on reported outcomes from existing systematic reviews.

Methods

Four electronic databases, PubMed, EMBASE, Epistimonikos and the Cochrane Database of Systematic Reviews were searched for articles on H1N1pdm09 vaccination published from 2009 to January 2021. Systematic reviews assessing short- or long-term adverse events after H1N1pdm09 vaccination were considered for inclusion. Data was extracted from all selected reviews. Outcomes were grouped and results from each included review were presented narratively and in tables.

Results

16 systematic reviews met the inclusion criteria. Reported outcomes were short-term events (3 reviews), fetal/pregnancy outcomes (8 reviews), Guillain-Barré syndrome (GBS) (4 reviews), narcolepsy (2 reviews) demyelinating diseases (1 review based on one study only) and inflammatory bowel disease (IBD) (1 review). Short-term serious adverse events were rare, 3 cases amongst 16725 subjects in 18 randomized controlled trials (0.018%). No deaths were reported. The risks of local events were generally higher for adjuvanted vaccines as compared to unadjuvanted vaccines. Maternal H1N1pdm09 vaccination in any trimester was not associated with an increase in preterm birth, small for gestational age, congenital malformations or fetal death. For GBS, results were conflicting. The main systematic review on narcolepsy found a 5-14-fold increased risk in children, and a 2-7- fold increased risk in adults after vaccination with Pandemrix. The attributable risk of narcolepsy one year after vaccination was 1 case per 18 400 vaccine doses in children/adolescents, and 1 case per 181 000 vaccine doses in adults.

Conclusion

Adjuvanted vaccines had more local but not serious adverse events compared to unadjuvanted vaccines. Vaccination with Pandemrix was strongly associated with narcolepsy, particularly in children. No increased risks of pregnancy outcomes were seen after pandemic vaccination. The findings on GBS were inconclusive.
Polymorphisms of the FCN2 Gene 3’UTR Region and Their Clinical Associations in Preterm Newborns
Authors: Świerzko Anna S. , Jarych Dariusz , Gajek Gabriela , Chojnacka Karolina , Kobiela Paulina , Kufelnicka-Babout Maja , Michalski Mateusz , Sobczuk Katarzyna , Szala-Poździej Agnieszka , Matsushita Misao , Mazela Jan , Domżalska-Popadiuk Iwona , Kilpatrick David C. , Kalinka Jarosław , Sekine Hideharu , Cedzyński Maciej
Published: October 2021

Ficolin-2 is regarded as an important innate immunity factor endowed with both lectin (carbohydrate recognition) qualities and ability to induce complement activation. The aim of this study was to investigate the association of the FCN2 3’-untranslated region (3’UTR) polymorphisms with ficolin-2 expression and perinatal complications in preterm neonates. The sequencing analysis allowed us to identify six 3’UTR polymorphisms with minor allele frequency (MAF) >1%: rs4521835, rs73664188, rs11103564, rs11103565, rs6537958 and rs6537959. Except for rs4521835, all adhered to Hardy-Weinberg expectations. Moreover, rs6537958 and rs6537959 were shown to be in perfect linkage disequilibrium (LD) with nine other genetic polymorphisms: rs7040372, rs7046516, rs747422, rs7847431, rs6537957, rs6537960, rs6537962, rs11462298 and rs7860507 together stretched on a distance of 1242 bp and very high LD with rs11103565. The 3’UTR region was shown to bind nuclear extract proteins. The polymorphisms at rs4521835 and rs73664188 were found to influence serum ficolin-2 concentration significantly. All polymorphisms identified create (together with exon 8 polymorphism, rs7851696) two haplotype blocks. Among 49 diplotypes (D1-D49) created from rs7851696 (G>T), rs4521835 (T>G), rs73664188 (T>C), rs11103564 (T>C), rs11103565 (G>A) and rs6537959 (T>A), twenty two occurred with frequency >1%. Two diplotypes: D13 (GTTTGT/GGTCGT) and D10 (GTTTGT/GGTCGA), were significantly more frequent among preterm neonates with early onset of infection and pneumonia, compared with newborns with no infectious complications (OR 2.69 and 2.81, respectively; both p<0.05). The minor (C) allele at rs73664188 was associated with an increased risk of very low (≤1500 g) birthweight (OR=1.95, p=0.042) but was associated with the opposite effect at rs11103564 (OR=0.11, p=0.005).
Design and Immunological Validation of Macaca fascicularis Papillomavirus Type 3 Based Vaccine Candidates in Outbred Mice: Basis for Future Testing of a Therapeutic Papillomavirus Vaccine in NHPs
Authors: Neckermann Patrick , Boilesen Ditte Rahbaek , Willert Torsten , Pertl Cordula , Schrödel Silke , Thirion Christian , Asbach Benedikt , Holst Peter Johannes , Wagner Ralf
Published: October 2021

Persistent human papillomavirus (HPV) infections are causative for cervical neoplasia and carcinomas. Despite the availability of prophylactic vaccines, morbidity and mortality induced by HPV are still too high. Thus, an efficient therapy, such as a therapeutic vaccine, is urgently required. Herein, we describe the development and validation of Macaca fascicularis papillomavirus type 3 (MfPV3) antigens delivered via nucleic-acid and adenoviral vectors in outbred mouse models. Ten artificially fused polypeptides comprising early viral regulatory proteins were designed and optionally linked to the T cell adjuvant MHC-II-associated invariant chain. Transfected HEK293 cells and A549 cells transduced with recombinant adenoviruses expressing the same panel of artificial antigens proved proper and comparable expression, respectively. Immunization of outbred CD1 and OF1 mice led to CD8+ and CD4+ T cell responses against MfPV3 antigens after DNA- and adenoviral vector delivery. Moreover, in vivo cytotoxicity of vaccine-induced CD8+ T cells was demonstrated in BALB/c mice by quantifying specific killing of transferred peptide-pulsed syngeneic target cells. The use of the invariant chain as T cell adjuvant enhanced the T cell responses regarding cytotoxicity and in vitro analysis suggested an accelerated turnover of the antigens as causative. Notably, the fusion-polypeptide elicited the same level of T-cell responses as administration of the antigens individually, suggesting no loss of immunogenicity by fusing multiple proteins in one vaccine construct. These data support further development of the vaccine candidates in a follow up efficacy study in persistently infected Macaca fascicularis monkeys to assess their potential to eliminate pre-malignant papillomavirus infections, eventually instructing the design of an analogous therapeutic HPV vaccine.
Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio
Authors: Mansour Adel Abo , Raucci Federica , Saviano Anella , Tull Samantha , Maione Francesco , Iqbal Asif Jilani
Published: October 2021

Gout is caused by depositing monosodium urate (MSU) crystals within the articular area. The infiltration of neutrophils and monocytes drives the initial inflammatory response followed by lymphocytes. Interestingly, emerging evidence supports the view that in situ imbalance of T helper 17 cells (Th17)/regulatory T cells (Treg) impacts the subsequent damage to target tissues. Galectin-9 (Gal-9) is a modulator of innate and adaptive immunity with both pro- and anti-inflammatory functions, dependent upon its expression and cellular location. However, the specific cellular and molecular mechanisms by which Gal-9 modulates the inflammatory response in the onset and progression of gouty arthritis has yet to be elucidated. In this study, we sought to comprehensively characterise the functional role of exogenous Gal-9 in an in vivo model of MSU crystal-induced gouty inflammation by monitoring in situ neutrophils, monocytes and Th17/Treg recruited phenotypes and related cyto-chemokines profile. Treatment with Gal-9 revealed a dose-dependent reduction in joint inflammation scores, knee joint oedema and expression of different pro-inflammatory cyto-chemokines. Furthermore, flow cytometry analysis highlighted a significant modulation of infiltrating inflammatory monocytes (CD11b+/CD115+/LY6-Chi) and Th17 (CD4+/IL-17+)/Treg (CD4+/CD25+/FOXP-3+) cells following Gal-9 treatment. Collectively the results presented in this study indicate that the administration of Gal-9 could provide a new therapeutic strategy for preventing tissue damage in gouty arthritic inflammation and, possibly, in other inflammatory-based diseases.
Functional Activity of the Complement System in Hospitalized COVID-19 Patients: A Prospective Cohort Study
Authors: Charitos Panteleimon , Heijnen Ingmar A. F. M. , Egli Adrian , Bassetti Stefano , Trendelenburg Marten , Osthoff Michael
Published: October 2021

Aims

Although the exact factors promoting disease progression in COVID-19 are not fully elucidated, unregulated activation of the complement system (CS) seems to play a crucial role in the pathogenesis of acute lung injury (ALI) induced by SARS-CoV-2. In particular, the lectin pathway (LP) has been implicated in previous autopsy studies. The primary purpose of our study is to investigate the role of the CS in hospitalized COVID-19 patients with varying degrees of disease severity.

Methods

In a single-center prospective observational study, 154 hospitalized patients with PCR-confirmed SARS-CoV-2 infection were included. Serum samples on admission to the COVID-19 ward were collected for analysis of CS pathway activities and concentrations of LP proteins [mannose-binding lectin (MBL) and ficolin-3 (FCN-3)] & C1 esterase inhibitor (C1IHN). The primary outcome was mechanical ventilation or in-hospital death.

Results

The patients were predominately male and had multiple comorbidities. ICU admission was required in 16% of the patients and death (3%) or mechanical ventilation occurred in 23 patients (15%). There was no significant difference in LP activity, MBL and FCN-3 concentrations according to different peak disease severities. The median alternative pathway (AP) activity was significantly lower (65%, IQR 50-94) in patients with death/invasive ventilation compared to patients without (87%, IQR 68-102, p=0.026). An optimal threshold of <65.5% for AP activity was derived from a ROC curve resulting in increased odds for death or mechanical ventilation (OR 4,93; 95% CI 1.70-14.33, p=0.003) even after adjustment for confounding factors. Classical pathway (CP) activity was slightly lower in patients with more severe disease (median 101% for death/mechanical ventilation vs 109%, p=0.014). C1INH concentration correlated positively with length of stay, inflammatory markers and disease severity on admission but not during follow-up.

Conclusion

Our results point to an overactivated AP in critically ill COVID-19 patients in vivo leading to complement consumption and consequently to a significantly reduced AP activity in vitro. The LP does not seem to play a role in the progression to severe COVID-19. Apart from its acute phase reaction the significance of C1INH in COVID-19 requires further studies.
Can Environmental Conditions at North Atlantic Deep-Sea Habitats Be Predicted Several Years Ahead? ——Taking Sponge Habitats as an Example
Authors: Liu Feifei , Daewel Ute , Samuelsen Annette , Brune Sebastian , Hanz Ulrike , Pohlmann Holger , Baehr Johanna , Schrum Corinna
Published: October 2021

Predicting the ambient environmental conditions in the coming several years to one decade is of key relevance for elucidating how deep-sea habitats, like for example sponge habitats, in the North Atlantic will evolve under near-future climate change. However, it is still not well known to what extent the deep-sea environmental properties can be predicted in advance. A regional downscaling prediction system is developed to assess the potential predictability of the North Atlantic deep-sea environmental factors. The large-scale climate variability predicted with the coupled Max Planck Institute Earth System Model with low-resolution configuration (MPI-ESM-LR) is dynamically downscaled to the North Atlantic by providing surface and lateral boundary conditions to the regional coupled physical-ecosystem model HYCOM-ECOSMO. Model results of two physical fields (temperature and salinity) and two biogeochemical fields (concentrations of silicate and oxygen) over 21 sponge habitats are taken as an example to assess the ability of the downscaling system to predict the interannual to decadal variations of the environmental properties based on ensembles of retrospective predictions over the period from 1985 to 2014. The ensemble simulations reveal skillful predictions of the environmental conditions several years in advance with distinct regional differences. In areas closely tied to large-scale climate variability and ice dynamics, both the physical and biogeochemical fields can be skillfully predicted more than 4 years ahead, while in areas under strong influence of upper oceans or open boundaries, the predictive skill for both fields is limited to a maximum of 2 years. The simulations suggest higher predictability for the biogeochemical fields than for the physical fields, which can be partly attributed to the longer persistence of the former fields. Predictability is improved by initialization in areas away from the influence of Mediterranean outflow and areas with weak coupling between the upper and deep oceans. Our study highlights the ability of the downscaling regional system to predict the environmental variations at deep-sea benthic habitats on time scales of management relevance. The downscaling system therefore will be an important part of an integrated approach towards the preservation and sustainable exploitation of the North Atlantic benthic habitats.
A Simplified Approach for Evaluating the Flexural Response of Concrete Beams Reinforced With FRP Bars
Authors: Sun Rui , Perera Ricardo , Gu Jintao , Wang Yi
Published: October 2021

This paper is proposed for modelling concrete beams reinforced with fiber reinforced polymer (FRP) bars in a simplified way. In order to appropriately model the FRP-reinforced concrete beams the stiffness matrix is developed in the frequency domain using fast Fourier Transform. Numerical results with the proposed spectral model for the load-displacement response and the shear stress distribution between FRP reinforcement and surrounding concrete are obtained for beams statically tested. Tens of elements are deployed in this work due to the simplicity of the proposed model. Using the same spectral model the natural frequency and mode shapes are evaluated since the frequency-dependent stiffness matrix enables it to apply for dynamic study, e.g. modal analysis. The feasibility of the proposed numerical approach for performing dynamic analysis especially for high frequency excitations in an efficient way makes it a promising tool for use in the field of structural health monitoring according to the changes in dynamic characteristics.
Case Report: Streptococcus Suis Meningitis Diagnosed in a HIV-Infected Patient With Cryptococcal Meningitis Using Next-Generation Sequencing
Authors: Xie Yirui , Ruan Bing , Lang Guanjing , Zhu Biao
Published: October 2021

Background: Streptococcus suis has been recognized as a zoonotic pathogen that may cause infections in humans. Although rarely described, it is not surprising that both cryptococcal and streptococcus suis meningitis infections can co-exist in a HIV-infected patient with a low CD4 count. However, a fast and accurate diagnose of meningitis of multipathogenic infections is challenging. In this report, we describe such a case of a HIV-infected patient with meningitis of multipathogenic infections.

Case Presentation: The patient was a 34-year-old Chinese male who was diagnosed with cryptococcal meningitis and HIV at the same time about 1 year ago. During the same time period, he had received (with good compliance) fluconazole and tenofovir-lamivudine- dolutegravir based antiretroviral therapy (ART). However, symptom of progressively worsening occipital headache appeared after he was exposed to a truck which was used for transporting pigs. Initial workup indicated an increase of the cerebrospinal fluid (CSF) opening pressure (OP) and an increase in the number of lymphocytes and proteins in CSF. A magnetic resonance imaging (MRI) scan revealed that partial cerebellar surface enhancement. The cryptococcus capsular antigen test of CSF was positive. The results of the India Ink microscopy for cryptococcus, nucleic acid of CMV and EBV and mycobacterium tuberculosis (MTB) tests of CSF were negative. The results of the bacteria and fungi smear and culture of CSF were also negative. Eventually, streptococcus suis was detected using next-generation sequencing (NGS) in CSF. The diagnosis of Streptococcus suis meningitis was made based on the patient's contact history with carrier pigs and the clinical findings addressed above. The treatment of 2 weeks of intravenous ceftriaxone and 1 week of oral moxifloxacin resulted in improvement of the condition of CSF. The anti-fungal treatment using fluconazole continued until the CFS OP went down to a normal level and the cryptococcus capsular antigen test of CSF was negative 6 months later.

Conclusion: This case highlights that NGS might be beneficial to HIV-infected patients who have meningitis with negative CSF culture results. Multiple etiologies for such condition in the immunocompromised patients must be taken into consideration and early stage NGS is recommended.
Preclinical Assessment of Paclitaxel- and Trastuzumab-Delivering Magnetic Nanoparticles Fe3O4 for Treatment and Imaging of HER2-Positive Breast Cancer
Authors: Guo Liting , Zhang Hongming , Liu Ping , Mi Tianai , Ha Da , Su Li , Huang Lei , Shi Yan , Zhang Jun
Published: October 2021

Objective: The purpose of this study was to investigate the anticancer activity and the potential imaging use of the innovative combination of magnetic nanoparticles (MNPs)-Fe3O4, paclitaxel (PTX), and trastuzumab (Herceptin) in HER2-positive breast cancer.

Methods: MNPs-Fe3O4 was synthesized and underwent water phase transfer and hydrophobic molecular loading, and its surface was then coupled with Herceptin mono-antibody. The morphological characteristics of MNPs-Fe3O4 were observed under transmission electron microscopy (TEM). Effects of PTX-Herceptin-MNPs-Fe3O4 on breast cancer cells were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide assay and the flow cytometric apoptosis assay. To establish a xenograft model, we injected breast cancer SK-BR-3 cells into the left thighs of nude mice. We measured the effect of PTX-Herceptin-MNPs-Fe3O4 on tumor growth by measuring tumor size and calculating inhibition rate with immunohistochemistry analysis further performed, and analyzed MNPs-Fe3O4 accumulation in tumor lesions using in vivo magnetic resonance imaging and in vivo fluorescence imaging.

Results: Most MNPs were in spherical shape of about 10 nm in diameter observed under TEM. PTX-Herceptin-MNPs-Fe3O4 showed greater cytotoxic effects, and induced a higher apoptosis rate of SK-BR-3 cells than all the other groups, with corresponding changes of apoptosis-related proteins. Meanwhile, the in vivo tumor xenograft model showed that tumor inhibition rate in the PTX-Herceptin-MNPs-Fe3O4 group was higher than in the PTX-Herceptin group. Furthermore, PTX-Herceptin-MNPs-Fe3O4 enhanced the T2 imaging contrast enhancement effect on tumors in tumor-bearing mice.

Conclusion: The novel PTX-Herceptin-MNPs-Fe3O4 combination may represent a promising alternative breast cancer treatment strategy and may facilitate tumor imaging.
Exploring Links Between Industrialization, Urbanization, and Chinese Inflammatory Bowel Disease
Authors: Cui Guanglin , Liu Hanzhe , Xu Gang , Laugsand Jann-Birger , Pang Zhigang
Published: October 2021

Background: Evidence is emerging that the incidence of inflammatory bowel diseases (IBD) is dramatically increased in China, but with a geographic variation.

Objectives: We performed a review to summarize the link of accelerated industrialization, urbanization to changing trends in the incidence of IBD over the last three decades.

Methods: An electronic database search was performed in PubMed, Medline, EMBASE and Google Scholar (for English literature) and the China Science Periodical Database in Wanfang Data (for Chinese literature) from January 1990 to June 2020.

Results: By systematically analyzing the changing trends of gross domestic product (GDP) or GDP per capita, population migration from rural areas to cities and increasing incidence of IBD in parallel in different Chinese regions, an association between accelerated industrialization and urbanization and rising rate of IBD was shown. In which, rates of IBD incidence were higher in provinces with a high value of GDP per capita than those provinces with a low value of GDP per capita. Analysis of available epidemiological data revealed that the incidence of IBD was rising in parallel with increasing trends of both gross products of industry and urban population in Yunnan Province in a 14-year interval. Further evidence suggested that industrialization- and urbanization-induced subsequent changes in environmental factors, e.g., Westernized dietary habits and obesity, and work-related stress, might contribute to the increased risk of IBD in China. In addition, the preliminary results showed that urbanization and Westernized dietary habits might induce significant changes in gut microbiota profile that are possibly to increase the risk for IBD in Chinese.

Conclusions: Existing evidence to suggest that accelerated industrialization/urbanization is associated with the increasing incidence of IBD in China, which provides novel insights to study the possible mechanisms for the recent increasing incidence of IBD in newly industrialized and urbanized developing countries. In the future, the interaction between relevant environmental factors e.g., air/water pollution and IBD susceptibility genes in Chinese should be examined.
Admission Blood Glucose Is Associated With the 30-Days Mortality in Septic Patients: A Retrospective Cohort Study
Authors: Wei Xiaoyuan , Min Yu , Yu Jiangchuan , Wang Qianli , Wang Han , Li Shuang , Su Li
Published: October 2021

Background: Sepsis, as one of the severe diseases, is frequently observed in critically ill patients, especially concurrent with diabetes. Whether admission blood glucose is associated with the prognosis, and outcome of septic patients is still debatable.

Methods: We retrospectively reviewed and analyzed the demographic characteristics of septic patients in the Medical Information Mart for Intensive Care III (MIMIC III, version 1.4) between June 2001 and October 2012. The Chi-square and Fisher's exact tests were used for the comparison of qualitative variables among septic patients with different glucose levels and the 30-day mortality in septic patients with diabetes or not. Univariate and stepwise multivariate Cox regression analyses were used to determine the risk factors for 30-day mortality. Kaplan-Meier analysis was conducted to reveal the different 30-day survival probabilities in each subgroup.

Results: A total of 2,948 septic patients (910 cases with diabetes, 2,038 cases without diabetes) were ultimately included in the study. The 30-day mortality was 32.4% (956/2,948 cases) in the overall population without any difference among diabetic and non-diabetic septic patients (p = 1.000). Admission blood glucose levels <70 mg/dl were only observed to be significantly associated with the 30-day mortality of septic patients without diabetes (hazard ratio (HR) = 2.48, p < 0.001). After adjusting for confounders, age >65 years (HR = 1.53, p = 0.001), the Sequential Organ Failure Assessment (SOFA) score >5 (HR = 2.26, p < 0.001), lactic acid >2 mmol/L (Lac, HR = 1.35, p = 0.024), and platelet abnormality (<100 k/ul: HR = 1.49; >300 k/ul: HR = 1.36, p < 0.001) were the independent risk factors for 30-day mortality in septic patients with diabetes. In non-diabetes population, age >65 years (HR = 1.53, p < 0.001), non-White or non-Black patients (HR = 1.30, p = 0.004), SOFA score >5 (HR = 1.56, p < 0.001), blood glucose <70 mg/dl (HR = 1.91, p = 0.003), anion gap (AG) >2 mmol/L (HR = 1.60, p < 0.001), Lac (HR = 1.61, p < 0.001), urea nitrogen >21 mg/dl (HR = 1.45, p = 0.001), alanine aminotransferase (ALT, HR = 1.31, p = 0.009), total bilirubin >1.2 mg/dl (HR = 1.20, p = 0.033), and low hemoglobin (HR = 1.34, p = 0.001) were the independent risk factors for 30-day mortality.

Conclusions: Our results indicate admission blood glucose, especially in terms of <70 mg/dl, is the key signaling in predicting the worse 30-day survival probability of septic patients without diabetes, which could help clinicians to make a more suitable and precise treatment modality in dealing with septic patients.
Clinical and Ultrasound Biomicroscopic Characteristics of Congenital Fibrovascular Pupillary Membrane-Induced Secondary Glaucoma
Authors: Zhu Yingting , Fang Lei , Zhong Yimin , Oatts Julius , Han Ying , Lin Shufen , Chen Liming , Zhou Xiaodi , Su Yihua , Liu Pingping , Liu Xing
Published: October 2021

Purpose: The purpose of this study was to describe and summarize the clinical features of congenital fibrovascular pupillary membrane-induced secondary glaucoma (CFPMSG).

Design: Cross-sectional case series.

Methods: Eyes of 32 patients with CFPMSG were enrolled. Demographic data, including gender, laterality, age at presentation, and age at onset of glaucoma were collected. Patients underwent comprehensive ophthalmic examinations and ultrasound biomicroscopy (UBM). CFPMSG eyes were classified into three groups based on UBM findings and intergroup analysis was performed using ANOVA.

Results: The average age at presentation was 2.4 ± 4.6 months (mean ± SD) and at glaucoma onset was 3.8 ± 4.5 months. Compared to normal fellow eyes, all affected eyes had increased intraocular pressure (IOP), axial length, corneal diameter, and central corneal thickness, and decreased anterior chamber depth (ACD) (all P ≤ 0.001). Twenty-two affected eyes (68.8%) had evidence of glaucomatous optic neuropathy. Based on iris configuration on UBM, eyes were classified as 53% type I (“U” shape), 34% type II (“Y” shape), and 13% type III (no anterior chamber). IOP in types II (33.8 ± 5.9 mmHg) and III (35.2 ± 5.9 mmHg) was significantly higher than in type I eyes (26.5 ± 5.1 mmHg). The ACD was shallower in type II compared to type I (P = 0.045).

Conclusion: Congenital fibrovascular pupillary membrane-induced secondary glaucoma is characterized by ocular hypertension, corneal enlargement and edema, axial length elongation, and glaucomatous optic neuropathy. Glaucoma in this condition is secondary to pupillary block and angle-closure. UBM provides important information for the diagnosis and classification of CFPMSG. This novel classification system demonstrated varying levels of severity and may guide on management of this disease.
Intramural Ectopic Pregnancy: Clinical Characteristics, Risk Factors for Uterine Rupture and Hysterectomy
Authors: Chen Xiaoran , Gao Lingyun , Yu Hongna , Liu Meijuan , Kong Shujun , Li Sijian
Published: October 2021

Background: Intramural ectopic pregnancy is defined as the gestational sac (GS) is entirely within the myometrium, separate from the endometrial cavity and fallopian tubes, which is unsustainable and potentially life-threatening. The data investigating the clinical characteristics, management strategy, and fertility outcomes after treatment of intramural ectopic pregnancies are very limited due to its extreme rarity.

Methods: To investigate the clinical characteristics, treatment options, and fertility outcomes in patients with intramural ectopic pregnancy, a retrospective study included 56 patients was conducted. We also used logistic regression to identify potential risk factors for uterine rupture and hysterectomy in these patients.

Results: The mean age of patients was 31.1 years, with an average gestational age (GA) of 10.0 weeks, and the majority of the patient cohort (83.9%) had uterine or endometrial surgical history. 55.4% of the intramural pregnancy was diagnosed by preoperative imaging examination and 67.7% was detected by ultrasound. There was no dominant predisposed zone of the GS. Common treatment strategies included laparotomy surgery (41.1%) and laparoscopic surgery (35.7%), followed by methotrexate (7.1%) and expectant management (5.4%). Uterine rupture occurred in 9 patients and 8 patients underwent a hysterectomy, but no maternal demise was found. Logistic regression showed that a GA >10 weeks predicted a significantly higher risk of uterine rupture (Odds ratio [OR] 8.000, 95% confidence interval [CI] 1.456–43.966, P = 0.017) and hysterectomy (OR 12.333, 95% CI 2.125–71.565, P = 0.005), and GS located in the fundus also predicted higher probability of uterine rupture (OR 7.000,95% CI 1.271–38.543, P = 0.025). Among the ten patients who had a desire for fertility, 6 of them succeeded and 4 of them successfully delivered with a GA ≥ 34 weeks.

Conclusion: GA > 10 weeks was the risk factor for both uterine rupture and hysterectomy, while patients with GS located in the uterine fundus had a significantly higher risk of uterine rupture. The fertility outcomes were moderate after treatment. The management strategies should be individualized according to disease conditions and the desire for fertility, and early diagnosis is essential for optimizing clinical outcomes.