bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights from Murine Studies on the Site Specificity of Atherosclerosis
Authors: Getz Godfrey S. , Reardon Catherine A.
Published: June 2024

Atherosclerosis is an inflammatory reaction that develops at specific regions within the artery wall and at specific sites of the arterial tree over a varying time frame in response to a variety of risk factors. The mechanisms that account for the interaction of systemic factors and atherosclerosis-susceptible regions of the arterial tree to mediate this site-specific development of atherosclerosis are not clear. The dynamics of blood flow has a major influence on where in the arterial tree atherosclerosis develops, priming the site for interactions with atherosclerotic risk factors and inducing cellular and molecular participants in atherogenesis. But how this accounts for lesion development at various locations along the vascular tree across differing time frames still requires additional study. Currently, murine models are favored for the experimental study of atherogenesis and provide the most insight into the mechanisms that may contribute to the development of atherosclerosis. Based largely on these studies, in this review, we discuss the role of hemodynamic shear stress, SR-B1, and other factors that may contribute to the site-specific development of atherosclerosis.
The Expression of Forkhead Box P3 T Regulatory Lymphocytes as a Prognostic Factor in Malignant Melanomas
Authors: Gâta Vlad Alexandru , Pașca Andrei , Roman Andrei , Muntean Maximilian Vlad , Morariu Dragoș Ștefan , Bonci Eduard Alexandru , Dina Constantin , Ungureanu Loredana
Published: June 2024

Since transcription factor Forkhead Box P3 (FoxP3) was identified as a specific regulatory T cell (Treg) marker, researchers have scrutinized its value as a potential novel therapeutic target or a prognostic factor in various types of cancer with inconsistent results. The present analysis was performed to assess the influence of Treg FoxP3 expression on the prognosis of primary melanoma and to evaluate the correlations with various clinicopathological prognostic factors. We analyzed all eligible patients with stage pT3 primary malignant melanomas treated in a tertiary cancer center. Immunohistochemical staining for Treg FoxP3 expression was performed on retrospectively identified paraffin blocks and subsequently correlated with the outcomes of the patients. A total of 81% of the patients presented a positive Treg FoxP3 expression, being correlated with a higher risk of lymph node metastasis, tumor relapse, and death. Moreover, positive expression was statistically associated with a shorter OS. The tumor relapse rate was estimated at 36.7%. A positive expression of Treg FoxP3 and lymph node metastasis were associated with a higher risk of death based on multivariate analysis. Treg FoxP3 expression may be used as an independent prognostic factor in patients with malignant melanoma to evaluate tumor progression and survival.
Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1
Authors: Hasegawa Kazuhiro , Tamaki Masanori , Sakamaki Yusuke , Wakino Shu
Published: June 2024

Nicotinamide adenine dinucleotide (NAD) is involved in renal physiology and is synthesized by nicotinamide mononucleotide adenylyltransferase (NMNAT). NMNAT exists as three isoforms, namely, NMNAT1, NMNAT2, and NMNAT3, encoded by Nmnat1, Nmnat2, and Nmnat3, respectively. In diabetic nephropathy (DN), NAD levels decrease, aggravating renal fibrosis. Conversely, sodium–glucose cotransporter-2 inhibitors increase NAD levels, mitigating renal fibrosis. In this regard, renal NAD synthesis has recently gained attention. However, the renal role of Nmnat in DN remains uncertain. Therefore, we investigated the role of Nmnat by establishing genetically engineered mice. Among the three isoforms, NMNAT1 levels were markedly reduced in the proximal tubules (PTs) of db/db mice. We examined the phenotypic changes in PT-specific Nmnat1 conditional knockout (CKO) mice. In CKO mice, Nmnat1 expression in PTs was downregulated when the tubules exhibited albuminuria, peritubular type IV collagen deposition, and mitochondrial ribosome (mitoribosome) excess. In CKO mice, Nmnat1 deficiency-induced mitoribosome excess hindered mitoribosomal translation of mitochondrial inner membrane-associated oxidative phosphorylation complex I (CI), CIII, CIV, and CV proteins and mitoribosomal dysfunction. Furthermore, the expression of hypermethylated in cancer 1, a transcription repressor, was downregulated in CKO mice, causing mitoribosome excess. Nmnat1 overexpression preserved mitoribosomal function, suggesting its protective role in DN.
Molecular Characterization of Chemosensory Protein (CSP) Genes and the Involvement of AgifCSP5 in the Perception of Host Location in the Aphid Parasitoid Aphidius gifuensis
Authors: Jiang Jun , Xue Jiayi , Yu Miaomiao , Jiang Xin , Cheng Yumeng , Wang Huijuan , Liu Yanxia , Dou Wei , Fan Jia , Chen Julian
Published: June 2024

Aphidius gifuensis is the dominant parasitic natural enemy of aphids. Elucidating the molecular mechanism of host recognition of A. gifuensis would improve its biological control effect. Chemosensory proteins (CSPs) play a crucial role in insect olfactory systems and are mainly involved in host localization. In this study, a total of nine CSPs of A. gifuensis with complete open reading frames were identified based on antennal transcriptome data. Phylogenetic analysis revealed that AgifCSPs were mainly clustered into three subgroups (AgifCSP1/2/7/8, AgifCSP3/9, and AgifCSP4/5/6). AgifCSP2/5 showed high expression in the antennae of both sexes. Moreover, AgifCSP5 was found to be specifically expressed in the antennae. In addition, fluorescent binding assays revealed that AifCSP5 had greater affinities for 7 of 32 volatile odor molecules from various sources. Molecular docking and site-directed mutagenesis results revealed that the residue at which AgifCSP5 binds to these seven plant volatiles is Tyr75. Behavior tests further confirmed that trans-2-nonenal, one of the seven active volatiles in the ligand binding test, significantly attracted female adults at a relatively low concentration of 10 mg/mL. In conclusion, AgifCSP5 may be involved in locating aphid-infested crops from long distances by detecting and binding trans-2-nonenal. These findings provide a theoretical foundation for further understanding the olfactory recognition mechanisms and indirect aphid localization behavior of A. gifuensis from long distances by first identifying the host plant of aphids.
Preparation of Conductive Cellulose Coated with Conductive Polymer and Its Application in the Detection of pH and Characteristic Substances in Sweat
Authors: Wu Yujia , Hou Defa , Zheng Yunwu , Lin Xu , Yang Fulin , Liu Can , Sun Hao
Published: June 2024

Rich biological information in sweat provides great potential for health monitoring and management. However, due to the complexity of sweat, the development of environmentally friendly green electronic products is of great significance to the construction of ecological civilization. This study utilized a simple combination of polystyrene sulfonate sodium (PSS) and filter paper (FP) to prepare cellulose materials coated with conductive polymers, developing an electrochemical sensor based on the modified materials. The mechanical and electrochemical properties of the fabricated PSS/FP membrane were optimized by adjusting the feeding dosage of PSS. The realized PSS/FP composite containing 7% PSS displayed good conductivity (9.1 × 10−2 S/m), reducing electric resistance by 99.2% compared with the original FP membrane (6.7 × 10−4 S/m). The stable current of the membrane in simulated sweat under different pH environments is highly correlated with the pH values. Additionally, when the membrane is exposed to simulated sweat with varying ion concentrations, the current signal changes in real time with the concentration variations. The response time averages around 0.3 s.
The Impact of Fine Particulate Matter on Embryonic Development
Authors: Wu Chia-Ta , Wu Ting-Shuan , Ku Min-Sho
Published: June 2024

Airborne fine particulate matter (PM2.5) in air pollution has become a significant global public health concern related to allergic diseases. Previous research indicates that PM2.5 not only affects the respiratory system but may also induce systemic inflammation in various tissues. Moreover, its impact may vary among different populations, with potential consequences during pregnancy and in newborns. However, the precise mechanisms through which PM2.5 induces inflammatory reactions remain unclear. This study aims to explore potential pathways of inflammatory responses induced by PM2.5 through animal models and zebrafish embryo experiments. In this study, zebrafish embryo experiments were conducted to analyze the effects of PM2.5 on embryo development and survival, and mouse experimental models were employed to assess the impact of PM2.5 stimulation on various aspects of mice. Wild-type zebrafish embryos were exposed to a PM2.5 environment of 25–400 μg/mL starting at 6 h after fertilization (6 hpf). At 6 days post-fertilization, the survival rates of the 25, 50, 100, and 200 µg/mL groups were 100%, 80, 40%, and 40%, respectively. Zebrafish embryos stimulated with 25 μg/mL of PM2.5 still exhibited successful development and hatching. Additionally, zebrafish subjected to doses of 25–200 μg/mL displayed abnormalities such as spinal curvature and internal swelling after hatching, indicating a significant impact of PM2.5 stimulation on embryo development. In the mouse model, mice exposed to PM2.5 exhibited apparent respiratory overreaction, infiltration of inflammatory cells into the lungs, elevated levels of inflammatory response-related cytokines, and inflammation in various organs, including the liver, lungs, and uterus. Blood tests on experimental mice revealed increased expression of inflammatory and chemotactic cytokines, and GSEA indicated the induction of various inflammatory responses and an upregulation of the TNF-α/NFκB pathway by PM2.5. Our results provide insights into the harmful effects of PM2.5 on embryos and organs. The induced inflammatory responses by PM2.5 may be mediated through the TNF-α/NFκB pathway, leading to systemic organ inflammation. However, whether PM2.5-induced inflammatory responses in various organs and abnormal embryo development are generated through different pathways requires further study to comprehensively clarify and identify potential treatment and prevention methods.
Effects of Different Doses of sUV-B Exposure on Taxane Compounds’ Metabolism in Taxus wallichiana var. Mairei
Authors: Zhong Weixue , Tian Xuchen , Zhang Ye , Tang Xiaoqing , Xiao Siqiu , Zhang Ying , Yang Jing , Liu Ying , Li Dewen
Published: June 2024

UV-B is an important environmental factor that differentially affects plant growth and secondary metabolites. The effects of supplemental ultraviolet-B (sUV-B) exposure (T1, 1.40 kJ·m−2·day−1; T2, 2.81 kJ·m−2·day−1; and T3, 5.62 kJ·m−2·day−1) on the growth biomass, physiological characteristics, and secondary metabolites were studied. Our results indicated that leaf thickness was significantly (p < 0.05) reduced under T3 relative to the control (natural light exposure, CK); The contents of 6-BA and IAA were significantly reduced (p < 0.05); and the contents of ABA, 10-deacetylbaccatin III, and baccatin III were significantly (p < 0.05) increased under T1 and T2. The paclitaxel content was the highest (0.036 ± 0.0018 mg·g−1) under T3. The cephalomannine content was significantly increased under T1. Hmgr gene expression was upregulated under T1 and T3. The gene expressions of Bapt and Dbtnbt were significantly (p < 0.05) upregulated under sUV-B exposure, and the gene expressions of CoA, Ts, and Dbat were significantly (p < 0.05) downregulated. A correlation analysis showed that the 6-BA content had a significantly (p < 0.05) positive correlation with Dbat gene expression. The IAA content had a significantly (p < 0.05) positive correlation with the gene expression of Hmgr, CoA, Ts, and Dbtnbt. The ABA content had a significantly (p < 0.05) positive correlation with Bapt gene expression. Dbat gene expression had a significantly (p < 0.05) positive correlation with the 10-deacetylbaccatin content. Hmgr gene expression was positively correlated with the contents of baccatin III and cephalomannine. Bapt gene expression had a significantly (p < 0.01) positive correlation with the paclitaxel content. A factor analysis showed that the accumulation of paclitaxel content was promoted under T2, which was helpful in clarifying the accumulation of taxane compounds after sUV-B exposure.
Molecular Mechanism Underlying ROS-Mediated AKH Resistance to Imidacloprid in Whitefly
Authors: Li Jingjing , Zhu Chaoqiang , Xu Yunhao , He Haifang , Zhao Chenchen , Yan Fengming
Published: June 2024

Synthetic insecticides used to control Bemisia tabaci include organophosphorus, pyrethroids, insect growth regulators, nicotinoids, and neonicotinoids. Among these, neonicotinoids have been used continuously, which has led to the emergence of high-level resistance to this class of chemical insecticides in the whitefly, making whitefly management difficult. The adipokinetic hormone gene (AKH) and reactive oxygen species (ROS) play roles in the development of insect resistance. Therefore, the roles of AKH and ROS in imidacloprid resistance in Bemisia tabaci Mediterranean (MED; formerly biotype Q) were evaluated in this study. The expression level of AKH in resistant B. tabaci MED was significantly lower than that in sensitive B. tabaci (MED) (p < 0.05). AKH expression showed a decreasing trend. After AKH silencing by RNAi, we found that ROS levels as well as the expression levels of the resistance gene CYP6CM1 and its upstream regulatory factors CREB, ERK, and P38 increased significantly (p < 0.05); additionally, whitefly resistance to imidacloprid increased and mortality decreased (p < 0.001). These results suggest that AKH regulates the expression of resistance genes via ROS in Bemisia tabaci.
Ophthalmic Complications, Diagnosis, and Treatment of Congenital Human Cytomegalovirus Infection
Authors: Modrzejewska Monika , Połubiński Piotr , Zdanowska Oliwia
Published: June 2024

Background: Human cytomegalovirus (hCMV) is the most common etiological agent of congenital infections seen in newborns. Among the most commonly observed complications in children with congenital human cytomegalovirus infection are those affecting the visual system. Ocular complications of congenital CMV (cCMV) are a topic rarely addressed in the literature, which prompted the authors to update the available knowledge with the latest data. Methodology: English-language literature published between April 2000 and November 2023 (PubMed, NIH, Google Scholar) was analyzed for ocular complications of cCMV. The data obtained were categorized according to the ocular area involved and the incidence. A compilation of criteria for the symptomatic form of cCMV was also created. Results: The cCMV complications described in the literature affect all parts of the visual system: the anterior segment, the posterior segment, the posterior visual pathways, and the visual cortex. The most commonly described ocular complication of cCMV is choroidal and retinal scarring. Conclusions: Ophthalmic complications of cCMV can cause severe visual disturbances. Ophthalmic diagnosis in newborns should include hCMV PCR testing, which has the highest sensitivity and specificity. In the symptomatic form of cCMV, treatment should be instituted according to recommendations. A consensus should be established for screening of primary hCMV infection in pregnant women, the way in which to define the symptomatic form of cCMV, and the appropriateness and standards of treatment for primary hCMV infection in pregnant women.
Predicting Major Adverse Carotid Cerebrovascular Events in Patients with Carotid Stenosis: Integrating a Panel of Plasma Protein Biomarkers and Clinical Features—A Pilot Study
Authors: Khan Hamzah , Zamzam Abdelrahman , Shaikh Farah , Saposnik Gustavo , Mamdani Muhammad , Qadura Mohammad
Published: June 2024

Background: Carotid stenosis (CS) is an atherosclerotic disease of the carotid artery that can lead to devastating cardiovascular outcomes such as stroke, disability, and death. The currently available treatment for CS is medical management through risk reduction, including control of hypertension, diabetes, and/or hypercholesterolemia. Surgical interventions are currently suggested for patients with symptomatic disease with stenosis >50%, where patients have suffered from a carotid-related event such as a cerebrovascular accident, or asymptomatic disease with stenosis >60% if the long-term risk of death is <3%. There is a lack of current plasma protein biomarkers available to predict patients at risk of such adverse events. Methods: In this study, we investigated several growth factors and biomarkers of inflammation as potential biomarkers for adverse CS events such as stroke, need for surgical intervention, myocardial infarction, and cardiovascular-related death. In this pilot study, we use a support vector machine (SVM), random forest models, and the following four significantly elevated biomarkers: C-X-C Motif Chemokine Ligand 6 (CXCL6); Interleukin-2 (IL-2); Galectin-9; and angiopoietin-like protein (ANGPTL4). Results: Our SVM model best predicted carotid cerebrovascular events with an area under the curve (AUC) of >0.8 and an accuracy of 0.88, demonstrating strong prognostic capability. Conclusions: Our SVM model may be used for risk stratification of patients with CS to determine those who may benefit from surgical intervention.
Fatigue, Internet Addiction and Symptoms of Long COVID—A Cross-Sectional Study of Polish Students
Authors: Zalewska Anna , Gałczyk Monika
Published: June 2024

Introduction: Fatigue is the most persistent symptom in patients with long COVID. Moreover, Internet addiction itself has become a pandemic long-term effect. The aim of this study was to investigate the degree of fatigue and Internet addiction in a group of students with COVID-19 and to determine the relationship between fatigue and addiction in students with and without long COVID symptoms. Material and methods: A cross-sectional study was conducted among 402 Polish students aged 19–26. The 183 students who had COVID-19 signaled the presence of long COVID symptoms, which corresponded to 45.5% of the surveyed group. The Modified Fatigue Impact Scale was used to assess the level of fatigue, and the Kimberly Young questionnaire was used to assess the level of Internet addiction. Results: 19.7% (95% c.i.: 15.9–23.9%) of the students surveyed had a moderate level of Internet dependence (Internet addiction measure value of 50 points or more). Most of them did not complain of high levels of fatigue. Higher levels of dependence and fatigue were observed in subjects with long COVID symptoms (MFIS mean value was 26.5 in this group vs. 17.7 in the others; p = 0.0000 ***). The higher the respondents’ level of dependence, the more they tended to feel tired (correlations were stronger in those with long COVID symptoms: rS = 0.23; p = 0.0017 **). Conclusions: In view of the results obtained, the study presented here has the potential to contribute to the international debate on the long-term health consequences of the COVID-19 pandemic and strategies to address them. The study provides data that may be useful in the development of educational and health policies that target the psychophysical well-being of patients with long COVID symptoms. This process should be considered as a long-term endeavor.
Nulliparas at Term with Premature Rupture of Membranes and an Unfavorable Cervix: Labor Induction with Prostaglandin or Oxytocin? A Retrospective Matched Case Study
Authors: Bas Lando Maayan , Majida Ewida , Solnica Amy , Helman Sarit , Margaliot Kalifa Tal , Grisaru-Granovsky Sorina , Reichman Orna
Published: June 2024

Background: Induction of labor (IOL) in nulliparas with premature rupture of membranes (PROM) and an unfavorable cervix at term poses challenges. Our study sought to investigate the impact of prostaglandin E2 (PGE2) compared to oxytocin on the duration of IOL in this specific group of parturients. Methods: This was retrospective matched-case study. All nulliparas with term PROM who underwent induction between January 2006 to April 2023 at Shaare Zedek Medical Center were identified. Cases induced by either PGE2 or oxytocin were matched by the following criteria: (1) time from PROM to IOL; (2) modified Bishop score prior to IOL ≤ 5; (3) newborn birthweight; and (4) vertex position. The primary outcome was time from IOL to delivery. Results: Ninety-five matched cases were identified. All had a modified Bishop score ≤ 5. Maternal age (26 ± 4.7 years old, p = 0.203) and gestational age at delivery (38.6 ± 0.6, p = 0.701) were similar between the groups. Matched factors including time from PROM to IOL (23.5 ± 19.2 versus 24.3 ± 21.4 p = 0.780), birth weight of the newborn (3111 g versus 3101 g, p = 0.842), and occiput anterior position (present on 98% in both groups p = 0.687) were similar. Time from IOL to delivery was significantly shorter by 3 h and 36 min in the group induced with oxytocin than in the group induced with PGE2 (p = 0.025). Within 24 h, 55 (58%) of those induced with PGE2 delivered, compared to 72 (76%) of those induced with oxytocin, (p = 0.033). The cesarean delivery rates [18 (19%) versus 17 (18%)], blood transfusion rates [2 (2%) versus 3 (3%)], and Apgar scores (8.8 versus 8.9) were similar between the groups (PGE2 versus oxytocin, respectively), p ≥ 0.387. Conclusions: Induction with oxytocin, among nulliparas with term PROM and an unfavorable cervix, was associated with a shorter time from IOL to delivery and a higher rate of vaginal delivery within 24 h, with no difference in short-term maternal or neonatal adverse outcomes.
Distinguishing Axillary Lymphadenopathy after COVID-19 Vaccination from Malignant Lymphadenopathy
Authors: Yamanaka Shintaro , Tanaka Keiko , Miyagawa Masao , Kido Teruhito , Hasebe Shinji , Yamamoto Shoichiro , Fujii Tomomi , Takeuchi Kazuto , Yakushijin Yoshihiro
Published: June 2024

Objectives: To study the differences between malignant hypermetabolic axillary lymphadenopathy (MHL) and COVID-19 vaccine-associated axillary hypermetabolic lymphadenopathy (VAHL) using clinical imaging. Methods: A total of 1096 patients underwent Positron Emission Tomography-Computed Tomography (PET-CT) between 1 June 2021 and 30 April 2022 at Ehime University Hospital. In total, 188 patients with axillary lymphadenopathy after the COVID-19 vaccination were evaluated. The patients were classified into three groups such as VAHL (n = 27), MHL (n = 21), and equivocal hypermetabolic axillary lymphadenopathy (EqHL; n = 140). Differences in lymph node (LN) swellings were statistically analyzed using clinical imaging (echography, CT, and 18F-FDG PET). Results: MHL included a higher female population (90.5%) owing to a higher frequency of breast cancer (80.9%). Axillary LNs of MHL did not show any LN fatty hilums (0%); however, those of VAHL and EqHL did (15.8 and 36%, respectively). After the logistic regression analysis of the patients who had axillary lymphadenopathy without any LN fatty hilums, the minor axis length and ellipticity (minor axis/major axis) in the largest axillary LN, SUVmax, and Tissue-to-Background Ratio (TBR) were useful in distinguishing malignant lymphadenopathies. A receiver-operating characteristic (ROC) analysis indicated that a cut-off value of ≥7.3 mm for the axillary LN minor axis (sensitivity: 0.714, specificity: 0.684) and of ≥0.671 for ellipticity (0.667 and 0.773, respectively) in the largest LN with the highest SUVmax and TBR were predictive of MHL. Conclusions: Axillary lymphadenopathy of the minor axis and ellipticity in LN without fatty hilums may be useful to be suspicious for malignancy, even in patients who have received COVID-19 vaccination. Further examinations, such as 18F-FDG PET, are recommended for such patients.
Impairment in Right Ventricular-Pulmonary Arterial Coupling in Overweight and Obesity
Authors: Goliopoulou Athina , Oikonomou Evangelos , Theofilis Panagiotis , Tsigkou Vasiliki , Makavos George , Kourampi Islam , Katsioupa Maria , Antoniou Vaios-Dionysios , Ikonomidis Ignatios , Lambadiari Vaia , Tsatsaragkou Aikaterini , Sarantos Savvas , Zakynthinos George E. , Vavuranakis Manolis , Siasos Gerasimos
Published: June 2024

Background: The association of obesity with right ventricular function and the interplay between right heart and pulmonary circulation is incompletely understood. We evaluate the role of obesity as a determinant of right ventricular-pulmonary artery coupling (RVAC). Methods: We retrospectively studied consecutive subjects without overt cardiovascular or pulmonary disease. Subjects were stratified according to body mass index (BMI) as normal weight, overweight, or obese. A transthoracic echocardiographic study was used to assess left and right heart functional and structural parameters. RVAC was assessed using the ratio of peak systolic velocity of the tricuspid annulus to pulmonary artery systolic pressure (PASP). Results: A total of 145 subjects were enrolled with diabetes mellitus incidence higher in obese. There was no difference in left ventricular global longitudinal strain and in PASP or markers of right ventricular systolic function based on BMI. RVAC was significantly lower in the presence of obesity (normal weight: 0.52 (0.19) cm·(sec·mmHg)−1 vs. overweight: 0.47 (0.16) cm·(sec·mmHg)−1 vs. obese: 0.43 (0.14) cm·(sec·mmHg)−1, p = 0.03), even after adjustment for confounders (β: −0.085, 95% confidence interval: −0.163, −0.009, p = 0.029). Conclusions: Our findings highlight the relationship between metabolic impairment and RVAC, suggesting additional mechanisms for heart failure development observed in obese subjects.
Accuracy of Estimated Fetal Weight Assessment in Fetuses with Congenital Diaphragmatic Hernia—Is the Hadlock Formula a Reliable Tool?
Authors: Kuchnowska Daria , Stachura Albert , Kosinski Przemyslaw , Gawlak Maciej , Wegrzyn Piotr
Published: June 2024

Objectives: Congenital diaphragmatic hernia (CDH) is defined as organ protrusion from the abdominal to the thoracic cavity. The Hadlock formula is the most commonly used tool for calculating estimated fetal weight (EFW). The anatomical nature of CDH usually leads to underestimation of the abdominal circumference, resulting in underestimation of fetal weight. Accurate weight estimation is essential before birth for counselling, preparation before surgery and ECMO. The research is made to compare the accuracy of Hadlock’s formula and Faschingbauer’s formula for fetal weight estimation in CDH fetuses population. Methods: In our study, we investigated differences between EFW and actual birthweight in 42 fetuses with CDH as compared to 80 healthy matched controls. EFW was calculated using the Hadlock formula and a recently introduced formula described by Faschingbauer et al., which was tailored for fetuses with CDH. Additionally, both of the formulas were adjusted for the interval between the ultrasound and delivery for both of the groups. Results: The majority of hernias were left-sided (92.8% vs. 7.2%). EFW adjusted for the interval between the ultrasound and delivery had the highest correlation with the actual birthweight in both, study group and controls. We compared the results for both tools and found the Hadlock formula to predict birthweight in CDH children with a 7.8 ± 5.5% error as compared to 7.9 ± 6.5% error for the Faschingbauer’s formula. Conclusions: The Hadlock formula adjusted for the interval between the ultrasound and delivery is a more precise method of calculating EFW in fetuses with CDH. Routine biometry scan using Hadlock’s formula remains reliable for predicting birthweight.
Electrochemical Enrichment of Biocharcoal Modified on Carbon Electrodes for the Detection of Nitrite and Paraxon Ethyl Pesticide
Authors: Adiraju Anurag , Brahem Amina , Lu Tianqi , Al-Hamry Ammar , Zhou Yu , Wei Leixin , Jalasutram Aditya , Tegenkamp Christoph , Halouani Kamel , Kanoun Olfa
Published: June 2024

Biocharcoal (BioC), a cost-effective, eco-friendly, and sustainable material can be derived from various organic sources including agricultural waste. However, to date, complex chemical treatments using harsh solvents or physical processes at elevated temperatures have been used to activate and enhance the functional groups of biochar. In this paper, we propose a novel easy and cost-effective activation method based on electrochemical cycling in buffer solutions to enhance the electrochemical performance of biocharcoal derived from almond shells (AS-BioC). The novel electrochemical activation method enhanced the functional groups and porosity on the surface of AS-BioC, as confirmed by microscopic, spectroscopic characterizations. Electrochemical characterization indicated an increase in the conductivity and surface area. A modified SPCE with activated AS-BioC (A.AS-BioC/SPCE), shows enhanced electrochemical performance towards oxidation and reduction of nitrite and paraxon ethyl pesticide, respectively. For both target analytes, the activated electrode demonstrates high electrocatalytic activity and achieves a very LOD of 0.38 µM for nitrite and 1.35 nM for ethyl paraxon with a broad linear range. The sensor was validated in real samples for both contaminants. Overall, the research demonstrates an innovative technique to improve the performance of AS-BioC to use as a modifier material for electrochemical sensors.
OptimalNN: A Neural Network Architecture to Monitor Chemical Contamination in Cancer Alley
Authors: Udeji Uchechukwu Leo , Margala Martin
Published: June 2024

The detrimental impact of toxic chemicals, gas, and oil spills in aquatic environments poses a severe threat to plants, animals, and human life. Regions such as Cancer Alley exemplify the profound consequences of inadequately controlled chemical spills, significantly affecting the local community. Given the far-reaching effects of these spills, it has become imperative to devise an efficient method for early monitoring, estimation, and cleanup, utilizing affordable and effective techniques. In this research, we explore the application of U-shaped neural Network (UNET) and U-shaped neural network transformer (UNETR) neural network models designed for the image segmentation of chemical and oil spills. Our models undergo training using the Commonwealth Scientific and Industrial Research Organization (CSIRO) dataset and the Oil Spill Detection dataset, employing a specialized filtering technique to enhance detection accuracy. We achieved training accuracies of 95.35% and 91% by applying UNET on the Oil Spill and the CSIRO datasets after 50 epochs of training, respectively. We also achieved a training accuracy of 75% by applying UNETR to the Oil Spill dataset. Additionally, we integrated mixed precision to expedite the model training process, thus maximizing data throughput. To further accelerate our implementation, we propose the utilization of the Field Programmable Gate Array (FPGA) architecture. The results obtained from our study demonstrate improvements in inference latency on FPGA.
The Relationship between Sense of Presence, Emotional Response, and Clinical Outcomes in Virtual Reality-Based Therapy for Treatment-Resistant Schizophrenia: An Exploratory Correlational Study
Authors: Augustin Elischa , Beaudoin Mélissa , Giguère Sabrina , Ziady Hind , Phraxayavong Kingsada , Dumais Alexandre
Published: June 2024

Avatar therapy (AT) is a novel virtual reality-based psychotherapy that has been developed to treat auditory verbal hallucinations (AVH) in treatment-resistant schizophrenia. Various psychotherapeutic components, such as emotions and sense of presence, could contribute to clinical outcomes. However, the interplay between sense of presence, emotions, and clinical response has seldom been investigated. This study aimed to explore the relations between sense of presence, emotions, and clinical outcomes in AT. To conduct this investigation, data from previous and ongoing AT trials were used. Sense of presence and emotions were assessed using standardized questionnaires. AVH were evaluated using the Psychotic Symptom Rating Scales. While sense of presence was positively associated with positive emotions such as control and serenity, no significant associations were found for negative emotions. Moreover, a higher level of sense of presence was associated with a bigger decrease in AVH. Overall, positive emotions seem to be associated with sense of presence in AT. Sense of presence also seems to be involved in the therapeutic outcome, thereby suggesting that this could be an important component related to clinical response. More studies are needed to confirm these trends, which could be generalized to other virtual reality-based psychotherapies.
The Relationship between Seropositive Rheumatoid Arthritis and Congestive Heart Failure: A Nationwide Longitudinal Cohort Study in Korea
Authors: Kim Yeo Song , Hong Je Beom , Kim Hakyung , Sheen Seung Hun , Han In-bo , Kim Jeong Gyun , Jeun Sin Soo , Sohn Seil
Published: June 2024

Objectives: The aim of this nationwide longitudinal cohort study is to determine the risk of congestive heart failure (CHF) associated with a seropositive rheumatoid arthritis (RA) population in Korea. Methods: In this study, National Health Insurance Service-Health Screening Cohort (NHIS-HEALS) data from 2002 to 2003 were used. The cohort was followed up with for 12 years until December of 2015. Seropositive RA was defined as a patient prescribed with a disease-modifying anti-rheumatic drug (DMARD) among patients with the International Classification of Diseases code M05 (seropositive RA). Patients who were diagnosed before 2004 were excluded. The seropositive RA group consisted of 2765 patients, and a total of 13,825 patients were in the control group. The Kaplan–Meier method was used to calculate the 12-year CHF incidence rate for each group. A Cox proportional hazards regression analysis was used to estimate the hazard ratio of CHF. Results: The hazard ratio of CHF in the seropositive RA group was 2.41 (95% confidence interval (CI): 1.40–4.14) after adjusting for age and sex. The adjusted hazard ratio of CHF in the seropositive RA group was 2.50 (95% CI: 1.45–4.30) after adjusting for age, sex, income, and comorbidities. In females aged ≥65 and aged <65, the incidence rates in the non-hypertension, non-diabetes mellitus, and non-dyslipidemia subgroups were significantly higher in the seropositive RA group than in the control group. Conclusions: This nationwide longitudinal cohort study shows an increased risk of CHF in patients with seropositive RA.
Therapeutic Effects of Engineered Exosomes from RAW264.7 Cells Overexpressing hsa-let-7i-5p against Sepsis in Mice—A Comparative Study with Human Placenta-Derived Mesenchymal Stem Cell Exosomes
Authors: Le Van Long , Chang Chao-Yuan , Chuang Ching-Wei , Syu Syuan-Hao , Shih Hung-Jen , Nguyen Vo Hong-Phuc , Van Minh Nguyen , Huang Chun-Jen
Published: June 2024

This study compared the therapeutic effects of engineered exosomes derived from RAW264.7 cells overexpressing hsa-let-7i-5p (engineered exosomes) to exosomes from human placenta-derived mesenchymal stem cells (hpMSC exosomes) against sepsis-induced acute lung injury. Adult male C57BL/6 mice were divided into lipopolysaccharide (LPS), LPS plus engineered exosome (LEExo), or LPS plus hpMSC exosome (LMExo) groups, alongside control groups. The results showed that lung injury scores (based on pathohistological characteristics) and the levels of lung function alterations, tissue edema, and leukocyte infiltration in LEExo and LMExo groups were comparable and significantly lower than in the LPS group (all p < 0.05). Furthermore, the levels of inflammation (nuclear factor-κB activation, cytokine upregulation), macrophage activation (hypoxia-inducible factor-1α activation, M1 phase polarization), oxidation, and apoptosis were diminished in LEExo and LMExo groups compared to the LPS group (all p < 0.05). Inhibition of hsa-let-7i-5p attenuated the therapeutic effects of both engineered and hpMSC exosomes. These findings underscore the potent therapeutic capacity of engineered exosomes enriched with hsa-let-7i-5p and their potential as an alternative to hpMSC exosomes for sepsis treatment. Continued research into the mechanisms of action and optimization of engineered exosomes could pave the way for their future clinical application.
Testing Mini-FLOTAC for the Monitorization of Gastrointestinal Parasitic Infections in Birds Kept at Four Iberian Zoological Institutions
Authors: Lozano João , Pombo Cándido , Salmo Rami , Cazapal-Monteiro Cristiana , Arias María Sol , Carvalho Daniela , Lordelo Madalena , Batista Augusto , Bernardino Rui , Rinaldi Laura , Oliveira Manuela , Paz-Silva Adolfo , Madeira de Carvalho Luís
Published: June 2024

Birds kept in zoological institutions are highly exposed to gastrointestinal (GI) parasitism caused by coccidia and nematodes. The current research aimed to characterize the avian GI parasitic fauna in several zoological collections in Portugal and Spain. During the full year of 2022, a total of 120 fecal samples were collected from four zoological institutions: Lisbon Zoo, Olivais Pedagogical Farm, and Avian Biodiversity Center (Lisbon, Portugal), and Avifauna park (Lugo, Spain). Analysis was conducted in domestic bird species (autochthonous and exotic poultry breeds), and 18 different exotic bird species like Galliformes (peacock, pheasant), Anseriformes (duck), Psittaciformes (parrot, macaw, cockatiel, parakeet, cockatoo), Coraciiformes (motmot), Charadriiformes (avocet), Strigiformes (owl), Phoenicopteriformes (flamingo), Struthioniformes (ostrich), Rheiformes (rhea), and Casuariiformes (emu, cassowary). Feces were processed using Mini-FLOTAC (MF), to identify parasitic forms and quantify their shedding (oocysts or eggs per gram of feces). Moreover, 15 fecal samples from pheasants were also processed using the McMaster method (McM), to compare the parasite shedding and frequencies between techniques. MF implementation allowed identification of coccidia infections in all bird collections. Also, peacocks of the Lisbon Zoo tested positive for Trichostrongylus tenuis and Strongyloides pavonis, and the exotic birds from Avifauna park were also positive for several nematode species, with Ascaridia sp., Capillaria sp., Strongyloides sp., and Syngamus trachea eggs being detected in pheasants’ feces. Moreover, the analysis of pheasants’ feces with MF detected prevalences of 33% for coccidia oocysts, and 47% for Capillaria sp. and Ascaridia sp. eggs, while McM detected prevalences of 13%, 27%, and 40% for the respective parasite taxa, with no differences being observed between methods (p = 0.39, p = 0.45, and p = 0.50, respectively). This research provided more scientific support regarding the importance of using Mini-FLOTAC in routine parasitological diagnosis in birds kept at zoological institutions.
A Simulation-Based Study on the Impact of Parametric Design on Outdoor Thermal Comfort and Urban Overheating
Authors: Wai Cheuk Yin , Tariq Muhammad Atiq Ur Rehman , Chau Hing-Wah , Muttil Nitin , Jamei Elmira
Published: June 2024

Under the current energy crisis and climate change, sustainable urban planning and building design are a priority to achieve a net-zero future, as energy use in buildings for thermal comfort is one of the major carbon emission contributors. To adapt to a rapidly growing and stringent urban environment, where buildings are causing more emissions due to more frequent and severe extreme hot weather events, the parametric design approach has great potential and flexibility in providing a sustainable solution by simulating different design scenarios. This study aims to analyse urban geometry and identify the impact of various built environment scenarios on outdoor thermal comfort under certain climates. The Grasshopper program was used along with the Ladybugs plug-in to provide visualised outcomes of outdoor thermal comfort, with simulation models on Rhinoceros 3D Version 7 SR37 (7.37.24107.1500). Comparing the thermal comfort performance of different design scenarios, based on building height, orientation and urban geometry, helps to identify which factors are more impactful on building design. This study demonstrates the workflow of parametric design in analysing the microclimate pattern and outdoor thermal comfort performance of the existing built environment in Melbourne, Australia, to provide an insight for stakeholders and builders to inform better decision-making in urban planning and building design in order to achieve a zero-emission future.
Effect of Water to Cement Ratio on Properties of Calcium Sulfoaluminate Cement Mortars
Authors: Gołaszewska Małgorzata , Gołaszewski Jacek , Chmiela Bartosz
Published: June 2024

Calcium sulfoaluminate (CSA) cements are a promising alternative to Portland clinker, however, a thorough understanding of their properties is needed for their broader use in the industry. One of the topics that requires a good understanding is the effect of the w/c ratio on the properties of CSA cements. To this end, the aim of this paper was to provide research into the effects of a w/c ratio in the range of 0.45–0.6 on the properties of fresh and hardened CSA pastes and mortars. For fresh mortars, consistency and setting time, as well as plastic shrinkage tests, were conducted, and were complemented by hydration heat tests, carried out on pastes. For hardened mortars, tests of compressive and flexural strength and dry shrinkage, as well as SEM photography, were conducted. It was found that, regardless of a higher hydration rate, the increase in w/c ratio decreased flexural and compressive strength, as well as shrinkage, while increasing consistency, setting time, and hydration heat. Also observed was a significant decrease in strength between 3 and 7 days of curing in mortars with a high w/c ratio. It can be concluded that, regardless of the hydration rate, low w/c ratios in CSA mortars provide better properties than high w/c ratios.
Segregated Conductive Polymer Composite with Fe3O4-Decorated Graphite Nanoparticles for Microwave Shielding
Authors: Matzui Ludmila Yu. , Syvolozhskyi Oleksii A. , Vovchenko Ludmila L. , Yakovenko Olena S. , Len Tetyana A. , Ischenko Olena V. , Vakaliuk Anna V. , Oliynyk Victor V. , Zagorodnii Volodymyr V. , Naumenko Antonina , Cojocari Maria , Fedorov Georgy , Kuzhir Polina
Published: June 2024

Graphite nanoplatelets (GNPs)—the segregated ultra-high molecular weight polyethylene (UHMWPE)-based composites with hybrid filler—decorated with Fe3O4 were developed. Using X-ray diffraction and scanning electron microscopy, it was shown that the decorated component has the shape of separate granules, or their clusters were distributed evenly over the GNPs surface. The individual Fe3O4 nanoparticles are predominantly rounded, with diameters of approximately 20–60 nm. The use of GNPs/Fe3O4 as a filler leads to significant decreases in the percolation limit φc, 0.97 vol% vs. 0.56 vol% for GNPs/UHMWPE- and (GNPs/Fe3O4)/UHMWPE segregated composite material (SCM), respectively. Modification of the GNP surface with Fe3O4 leads to an essential improvement in the electromagnetic interference shielding due to enhanced microwave absorption in the 26–37 GHz frequency range in its turn by abundant surface functional groups and lattice defects of GNPs/Fe3O4 nanoparticles.
Research on Thermal Stability and Flammability of Wood Scob-Based Loose-Fill Thermal Insulation Impregnated with Multicomponent Suspensions
Authors: Augaitis Nerijus , Vaitkus Saulius , Kairytė Agnė , Vėjelis Sigitas , Šeputytė-Jucikė Jurga , Balčiūnas Giedrius , Kremensas Arūnas
Published: June 2024

Loose-fill thermal composite insulation produced from surface-modified wood scobs has been explored as a potential fire-resistant material for building envelopes. This work involves fire resistance behavior comparisons between four coating systems consisting of liquid glass, liquid glass-tung oil, liquid glass-expandable graphite, and liquid glass-tung oil-expandable graphite. The techniques of thermogravimetric and differential thermogravimetric analyses, gross heat combustion via a calorimetric bomb, cone calorimetry, SEM imaging of char residues, and energy dispersive spectrometry for elemental analysis, as well as propensity to undergo continuous smoldering, were implemented. The coating technique resulted in greater thermal stability at a higher temperature range (500–650 °C) of the resulting loose-fill thermal composite insulation, reduced flame-damaged area heights after the exposure of samples at 45° for 15 s and 30 s, with a maximum of 49% decreased gross heat combustion, reduced heat release and total smoke release rates, improved char residue layer formation during combustion and changed smoldering behavior due to the formation of homogeneous and dense carbon layers. The results showed that the highest positive impact was obtained using the liquid glass and liquid glass-expandable graphite system because of the ability of the liquid glass to cover the wood scob particle surface and form a stable and strong expanding carbon layer.
Evaluation of the Acousto-Optic Figure of Merit and the Maximum Value of the Elasto-Optic Constant of Liquids
Authors: Nikitin Pavel A. , Pozhar Vitold E.
Published: June 2024

The elasto-optic properties of liquids on the basis of the first principles of acousto-optics were theoretically investigated. A relationship for calculating the elasto-optic constant of liquids using only the refractive index was obtained. The refractive index values corresponding to the maximum elasto-optic constant for polar and nonpolar liquids were determined. Calculations for about 100 liquids were performed and compared with known experimental data. This study significantly extends our understanding of the acousto-optic effect and has practical applications for predicting the elasto-optic constant of a liquid and estimating its wavelength dispersion.
Determination of Fluorine by Ion-Selective Electrode and High-Resolution Continuum Source Graphite Furnace Molecular Absorption Spectrometry with Respect to Animal Feed Safety
Authors: Kowalewska Zofia , Goluch Karolina , Korol Waldemar , Olchowski Rafał , Dobrowolski Ryszard
Published: June 2024

Fluorine, depending on its concentration and chemical form, is essential or toxic to humans and animals. Therefore, it is crucial to be able to determine it reliably. In this study, fluorine was determined in animal feed after extraction with HCl (gastric juice simulation). The standard potentiometric method with a fluoride-selective electrode (ISE) and newly developed high-resolution continuum source graphite furnace molecular absorption spectrometry (HR-CS GFMAS) method was applied. Feed samples turned out to be a challenge for HR-CS GFMAS. Chemical interferences (formation of competing molecules, CaF, GaCl, and GaP, instead of the target GaF molecule) and spectral effects (including a phosphorous molecule spectrum and atomic lines) were identified. An additional difficulty was caused by reagent contamination with F and memory effects. Difficulties were eliminated/reduced. The quality of ISE analysis was multi-directionally verified (including comprehensive proficiency testing). A risk of inaccuracy at low F concentration, where the calibration relationship is nonlinear, was investigated. The results of both methods were consistent, which confirms the accuracy of the methods and informs that the extracted fluorine is in fluoride form. The results of extensive ISE tests conducted in Poland in 2021–2023 have shown that, in most cases, the fluoride content is significantly lower than the threshold values.