bluuebunny/crossref_metadata_2025 huggingface dataset:
Children’s Independent Mobility: Current Knowledge, Future Directions, and Public Health Implications
Authors: Marzi Isabel , Reimers Anne Kerstin
Published: November 2018

Environmental changes significantly impact health behavior. Active travel behavior is mostly affected by increasing motorization, urban sprawl, and traffic safety. Especially for children, active and independent travel can contribute to physical activity, social and motor development, and other health-related outcomes. A reduced number of children engaging in independent mobility over the last 20 years demanded researchers to further examine the construct of children’s independent mobility. By examining relevant literature, this narrative review aims to provide the current state of knowledge on children’s independent mobility, and identify future directions in research, as well as practical implications. From a public health perspective, considering children’s independent mobility in intervention programs is recommended, since it is associated with numerous health and environmental benefits. To develop interventions, multilevel socio-ecological influences on children’s independent mobility are widely examined; however, evidence is limited due to heterogeneous measurements and a lack of high-quality prospective studies. To oppose the decline in children’s independent mobility, further analysis using comparable measures is needed to understand the determinants of children’s independent mobility and to enable international comparison.
Effect of Size, Shape and Map Background in Cartographic Visualization: Experimental Study on Czech and Chinese Populations
Authors: Stachoň Zdeněk , Šašinka Čeněk , Čeněk Jiří , Angsüsser Stephan , Kubíček Petr , Štěrba Zbyněk , Bilíková Martina
Published: November 2018

This paper deals with the issue of the perceptual aspects of selected graphic variables (specifically shape and size) and map background in cartographic visualization. The continued experimental study is based on previous findings and the presupposed cross-cultural universality of shape and size as a graphic variable. The results bring a new perspective on the usage of shape, size and presence/absence of background as graphic variables, as well as a comparison to previous studies. The results suggest that all examined variables influence the speed of processing. Respondents (Czech and Chinese, N = 69) identified target stimuli faster without a map background, with larger stimuli, and with triangular and circular shapes. Czech respondents were universally faster than Chinese respondents. The implications of our research were discussed, and further directions were outlined.
Melatonin Promotes Ubiquitination of Phosphorylated Pro-Apoptotic Protein Bcl-2-Interacting Mediator of Cell Death-Extra Long (BimEL) in Porcine Granulosa Cells
Authors: Wang Yingzheng , Zeng Shenming
Published: November 2018

Melatonin (N-acetyl-5-methoxytryptamine) is found in ovarian follicular fluid, and its concentration is closely related to follicular health status. Nevertheless, the molecular mechanisms underlying melatonin function in follicles are uncertain. In this study, melatonin concentration was measured in porcine follicular fluid at different stages of health. The melatonin concentration decreased as the follicles underwent atresia, suggesting that melatonin may participate in the maintenance of follicular health. The molecular pathway through which melatonin may regulate follicular development was further investigated. The pro-apoptotic protein BimEL (Bcl-2-interacting mediator of cell death-Extra Long), a key protein controlling granulosa cell apoptosis during follicular atresia, was selected as the target molecule. BimEL was downregulated when porcine granulosa cells were cultured in medium containing 10−9 M melatonin and isolated cumulus oocyte complexes (COCs) or follicle stimulating hormone (FSH). Interestingly, ERK-mediated phosphorylation was a prerequisite for the melatonin-induced decline in BimEL, and melatonin only promoted the ubiquitination of phosphorylated BimEL, and did not affect the activities of the lysosome or the proteasome. Moreover, the melatonin-induced downregulation of BimEL was independent of its receptor and its antioxidant properties. In conclusion, melatonin may maintain follicular health by inducing BimEL ubiquitination to inhibit the apoptosis of granulosa cells.
The Antitumor Natural Compound Falcarindiol Disrupts Neural Stem Cell Homeostasis by Suppressing Notch Pathway
Authors: Kim Tae-Jun , Kwon Hyun-Sook , Kang Mingyu , Leem Hyun Hee , Lee Kyung-Ha , Kim Do-Yeon
Published: November 2018

Neural stem cells (NSCs) are undifferentiated, multi-potent cells that can give rise to functional neurons and glial cells. The disruption in NSC homeostasis and/or the impaired neurogenesis lead to diverse neurological diseases, including depression, dementia, and neurodegenerative disorders. Falcarindiol (FAD) is a polyacetylene found in many plants, and FAD shows the cytotoxicity against breast cancers and colon cancers. However, there is no research on the consequence of FAD treatment in normal stem cells. Here, we suggest that FAD has anticancer roles against glioblastoma cells by inducing the differentiation of glioblastoma stem-like cells, as well as activating apoptosis pathway in glioblastoma cells. On the other hand, we also show that FAD has detrimental effects by disrupting the maintenance of normal NSCs and altering the balance between self-renewal and differentiation of NSCs.
A Comparative Study of Laser Doppler Vibrometers for Vibration Measurements on Pavement Materials
Authors: Hasheminejad Navid , Vuye Cedric , Van den bergh Wim , Dirckx Joris , Vanlanduit Steve
Published: November 2018

A laser Doppler vibrometer (LDV) is a noncontact optical measurement device to measure the vibration velocities of particular points on the surface of an object. Even though LDV has become more popular in road engineering in recent years, their signal-to-noise ratio (SNR) is strongly dependent on light scattering properties of the surface which, in some cases, needs to be properly conditioned. SNR is the main limitation in LDV instrumentation when measuring on low diffusive surfaces like pavements; therefore, an investigation on the SNR of different LDV devices on different surface conditions is of great importance. The objective of this research is to investigate the quality of two types of commercially available LDV systems—helium–neon (He–Ne)-based vibrometers and recently developed infrared vibrometers—on different surface conditions, i.e., retroreflective tape, white tape, black tape, and asphalt concrete. Both noise floor and modal analysis experiments are carried out on these surface conditions. It is shown that the noise floor of the He–Ne LDV is higher when dealing with a noncooperative dark surface, such as asphalt concrete, and it can be improved by improving the surface quality or by using an infrared LDV, which consequently improves the modal analysis experiments performed on pavement materials.
Fatty Acid and Related Potassium Kv2 Channel Blockers: Toxicity and Physiological Actions on Mosquitoes
Authors: Démares Fabien , Coquerel Quentin , Richoux Gary , Linthicum Kenneth , Bloomquist Jeffrey
Published: November 2018

Potassium channels constitute a very diverse group involved in neural signaling, neuronal activity, membrane potential maintenance, and action potential generation. Here, we tested the mammalian potassium channel blockers TRAM-34 and 5-hydroxydecanoate (5-HDC), as well as certain fatty acids (FA) that might fit in the lumen of the pore and block channel activity by obstructing K+ ion passage. Kv channel blockers could be leads for a novel pesticide type. Insecticidal activity was assessed by topical application to Anopheles gambiae adult mosquitoes, paralysis in a headless larval assay, at the cellular level with patch-clamp recordings of engineered HEK cells expressing AgKv2.1 channels, as well as central nervous system recordings from larval Drosophila melanogaster. With only one hydroxyl group difference, decanoic acid had a consistently greater effect than 5-HDC in blocking Kv channels, paralyzing larvae, and killing mosquitoes. The 11-dansylamino undecanoic acid (DAUDA) blockage of eukaryotic Kv channels is demonstrated for the first time, but it failed to kill adult mosquitoes. We synthesized alkyl esters from DAUDA and decanoic acid in an effort to improve cuticular penetration, but it had little impact upon adult toxicity. TRAM-34 and rolipram did not show activity on Kv channels nor potent insecticidal effect on adult mosquitoes. Furthermore, co-application of test compounds with permethrin did not increase mortality in adults. In conclusion, the compounds tested had modest insecticidal and synergistic activity.
Chemical and Non-Chemical Options for Managing Twospotted Spider Mite, Western Tarnished Plant Bug and Other Arthropod Pests in Strawberries
Authors: Dara Surendra , Peck David , Murray Dave
Published: November 2018

California strawberries have two major arthropod pests—the twospotted spider mite, Tetranychus urticae and the western tarnished plant bug, Lygus hesperus, which result in significant losses to the yield and quality of marketable berries. Other important insect pests that are frequently seen in strawberry include the greenhouse whitefly, Trialeurodes vaporariorum and the western flower thrips, Frankliniella occidentalis that cause varying levels of damage depending on the level of infestation. Chemical pesticides play a major role in managing these pests but not without the associated risk of pesticide resistance and environmental safety. Two field studies were conducted in commercial strawberry fields in Santa Maria, one of the strawberry growing areas in California Central Coast, to determine the efficacy of chemical, botanical and microbial pesticides in the integrated pest management (IPM) of strawberry. Chemical, botanical and microbial pesticides were evaluated against T. urticae in a small plot study in 2013 and against L. hesperus and other insect pests in a large plot study in 2015 in commercial strawberry fields. Bug vacuums were also used in the 2015 study. Results demonstrated that non-chemical alternatives can play an important role in strawberry IPM.
On an Elastoplastic Sliding Model for a Coated Single Asperity
Authors: Wang Can , Schipper Dik J.
Published: November 2018

In this study, a sliding friction model for coated single asperity contacts is proposed. A displacement-driven layered contact algorithm is firstly introduced and verified by the finite element method. Then, this algorithm is applied to simulate the contact between two semispherical asperities. The full sliding contact process is discretized into a series of transient steps, and each of these steps are calculated by the displacement-driven contact algorithm. The effects of the interference depth and the properties of, respectively, the tribofilm (thickness, elastic modulus, and yield strength) and the nanocrystalline layer on the sliding coefficient of friction are investigated. The results suggest that when surface adhesion and asperity damage are ignored, the plastic deformation of the tribofilm is the main source of the sliding friction. Greater interference depth, tribofilm with greater thickness, higher elastic modulus or lower yield strength, and the presence of a nanocrystalline layer will lead to a higher coefficient of friction in single asperity sliding.
Combined Damage Index to Detect Plastic Deformation in Metals Using Acoustic Emission and Nonlinear Ultrasonics
Authors: Zhang Lu , Oskoe Sepideh Karkouti , Li Hongyu , Ozevin Didem
Published: November 2018

Understanding the amount of degradation using nondestructive evaluation (NDE) methods provides an effective way of determining the fitness to service and the residual life of structural components. Due to uncertainties introduced by the single NDE method, a combined damage index using multi-sensor data increases the reliability of damage assessment. In this paper, the outputs of three NDE methods including acoustic emission (AE), linear ultrasonics (LUT), and nonlinear ultrasonics (NLUT) are merged to identify the amount of plastic deformation in aluminum 1100. The sensitivities of individual and combined methods to microstructural changes are evaluated. The coupon samples are loaded up to different strain levels and then unloaded. AE data is recorded in real time and ultrasonic data is recorded from the unloaded samples. The major features combined in the damage index are cumulative AE absolute energy and nonlinear coefficient. The microstructural state is verified with microscopic analysis and hardness testing. The developed damage index can nondestructively assess the amount of plastic deformation with higher reliability.
Teflon-on-Glass Molding Enables High-Throughput Fabrication of Hydrophilic-in-Hydrophobic Microwells for Bead-Based Digital Bioassays
Authors: Tripodi Lisa , Ven Karen , Kil Dries , Rutten Iene , Puers Robert , Lammertyn Jeroen
Published: November 2018

In recent years, Teflon-on-glass microwells have been successfully implemented in bead-based digital bioassays for the sensitive detection of single target molecules. Their hydrophilic-in-hydrophobic (HIH) nature enables the isolation and analysis of individual beads, carrying the target molecules, which can be further manipulated accurately through optical tweezer (OT) setups. However, these Teflon HIH-microwell platforms are conventionally fabricated through a complex, time-consuming and labor-intensive dry lift-off procedure which involves a series of major steps, limiting the up-scaling potential of these platforms. Alternative Teflon-based microwell fabrication methods have been extensively explored in literature but they preclude the generation of hydrophobic wells with hydrophilic bottom, thereby hampering the bioassay performance. Here, we present a new Teflon-on-glass molding method for the high throughput fabrication of hydrophilic-in-hydrophobic (HIH) microwell arrays, able to empower bead-based digital bioassays. Microwells 2.95 μm in depth and 3.86 μm in diameter were obtained to host individual beads. In these microwell arrays, sealing of reagents was demonstrated with an efficiency of 100% and seeding of superparamagnetic beads was achieved with an efficiency of 99.6%. The proposed method requires half as many steps when compared to the traditional dry lift-off process, is freely scalable and has the potential to be implemented in different bead-based bioassay applications.
Influence of Oxygen Vacancy Density on the Polaronic Configuration in Rutile
Authors: Liu Rulin , Fang Liang , Hao Yue , Chi Yaqing
Published: November 2018

Polaronic configurations that were introduced by oxygen vacancy in rutile TiO2 crystal have been studied by the DFT + U method. It is found that the building block of TiO6 will expand when extra electron is trapped in the central Ti atom as polaron. With manually adjusting the initial geometry of oxygen vacancy structure, a variety of polaronic configurations are obtained after variable-cell relaxation. By calculating different sizes of supercell model, it is found that the most stable configuration can be influenced by the density of oxygen vacancy. With increasing interaction between vacancies, the most stable polaronic configuration change from small polaronic configuration to mixed configuration.
Evolution of Electrochemical Cell Designs for In-Situ and Operando 3D Characterization
Authors: Tan Chun , Daemi Sohrab R. , Taiwo Oluwadamilola O. , Heenan Thomas M. M. , Brett Daniel J. L. , Shearing Paul R.
Published: November 2018

Lithium-based rechargeable batteries such as lithium-ion (Li-ion), lithium-sulfur (Li-S), and lithium-air (Li-air) cells typically consist of heterogenous porous electrodes. In recent years, there has been growing interest in the use of in-situ and operando micro-CT to capture their physical and chemical states in 3D. The development of in-situ electrochemical cells along with recent improvements in radiation sources have expanded the capabilities of micro-CT as a technique for longitudinal studies on operating mechanisms and degradation. In this paper, we present an overview of the capabilities of the current state of technology and demonstrate novel tomography cell designs we have developed to push the envelope of spatial and temporal resolution while maintaining good electrochemical performance. A bespoke PEEK in-situ cell was developed, which enabled imaging at a voxel resolution of ca. 230 nm and permitted the identification of sub-micron features within battery electrodes. To further improve the temporal resolution, future work will explore the use of iterative reconstruction algorithms, which require fewer angular projections for a comparable reconstruction.
ZnO Nanorods with High Photocatalytic and Antibacterial Activity under Solar Light Irradiation
Authors: Achouri Faouzi , Merlin Christophe , Corbel Serge , Alem Halima , Mathieu Laurence , Balan Lavinia , Medjahdi Ghouti , Ben Said Myriam , Ghrabi Ahmed , Schneider Raphaël
Published: November 2018

ZnO nanorods (NRs) with an average length and diameter of 186 and 20 nm, respectively, were prepared through a mild solvothermal route and used as photocatalysts either as dispersed powder or immobilized on glass slides. The ZnO NRs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Dispersed ZnO NRs and, to a lesser extent, immobilized ZnO NRs were demonstrated to exhibit high photocatalytic activity under simulated sunlight of low intensity (5.5 mW/cm2) both for the degradation of the Orange II dye and for Escherichia coli bacterial decontamination (2.5-fold survival decrease after 180 min irradiation for immobilized NRs). SEM, atomic force microscopy (AFM), fluorescence spectroscopy, and epifluorescence microscopy demonstrate that cell surface damages are responsible of bacterial inactivation. The immobilized ZnO NRs could be reused up to five times for bacterial decontamination at comparable efficiency and therefore have great potential for real environmental applications.
Comparison of Mechanical Properties of Natural Gut and Synthetic Polymer Harp Strings
Authors: Lynch-Aird Nicolas , Woodhouse Jim
Published: November 2018

The long-term mechanical behaviour of a number of fluorocarbon and gut harp strings has been examined, and the results compared with a previous study of rectified nylon strings. The stretching behaviour of the three materials was studied via different measures of the Young’s modulus; with test time scales on the order of weeks, minutes, and milliseconds. The strings were subjected to cyclic variations in temperature, enabling various aspects of their thermal behaviour to be investigated. The effects of humidity changes on gut strings were also examined. The behaviour of the fluorocarbon strings was found to be similar in many ways to that of the nylon strings, despite their different chemical formulation and significantly higher density. In particular, the faster measures of Young’s modulus were found to show an almost identical strong variation with the applied stress; while the thermal behaviour of both materials was largely determined by the balance between opposing effects associated with thermal contraction and thermal variations in the Young’s modulus. The gut strings showed some similarities of behaviour to the synthetic materials, but also major differences. All three measures of the Young’s modulus remained constant as the applied stress was increased. The gut strings were far more sensitive to changes in humidity than the synthetic materials, although some of the results, especially the thermal tuning sensitivity of the strings when held at constant length, displayed remarkable stability under changing humidity. The observed behaviour suggests very strongly that there is significant coupling between humidity-related changes in the linear density of a gut string and complementary changes in its tension.
Using Sensor-Based Quality Data in Automotive Supply Chains
Authors: Teucke Michael , Broda Eike , Börold Axel , Freitag Michael
Published: November 2018

In many current supply chains, transport processes are not yet being monitored concerning how they influence product quality. Sensor technologies combined with telematics and digital services allow for collecting environmental data to supervise these processes in near real-time. This article outlines an approach for integrating sensor-based quality data into supply chain event management (SCEM). The article describes relationships between environmental conditions and quality defects of automotive products and their mutual relations to sensor data. A discrete-event simulation shows that the use of sensor data in an event-driven control of material flows can keep inventory levels more stable. In conclusion, sensor data can improve quality monitoring in transport processes within automotive supply chains.
Applications of Big Data analytics and Related Technologies in Maintenance—Literature-Based Research
Authors: Baum Jens , Laroque Christoph , Oeser Benjamin , Skoogh Anders , Subramaniyan Mukund
Published: November 2018

Digitalisation is argued to increase the efficiency of maintenance activities in a production system. One consequence of digitalisation is data deluge; this allows data analytics methods and technologies to be used. However, the actual data analytical methods and technologies used may differ, thus leading to many scientific papers on this topic. The purpose of our contribution is to find and cluster scientific papers regarding the implemented approaches relevant for use in production maintenance. Our research is based on a broad, systematic literature review consisting of a two-step search approach combined with additional filtering and classification. Based on the search results, we evaluate and visualise the potential impact of data analytics on the subject of maintenance. The results of this study broadly summarise the research activities in production maintenance, whilst indicating that the impact of data analytics will grow further. Specific methodological approaches are clearly favored.
Exploring the pH-Induced Functional Phase Space of Human Serum Albumin by EPR Spectroscopy
Authors: Reichenwallner Jörg , Oehmichen Marie-T. , Schmelzer Christian E. H. , Hauenschild Till , Kerth Andreas , Hinderberger Dariush
Published: November 2018

A systematic study on the self-assembled solution system of human serum albumin (HSA) and paramagnetic doxyl stearic acid (5-DSA and 16-DSA) ligands is reported covering the broad pH range 0.7–12.9, mainly using electron paramagnetic resonance (EPR) methods. It is tested to which extent the pH-induced conformational isomers of HSA reveal themselves in continuous wave (CW) EPR spectra from this spin probing approach in comparison to an established spin-labeling strategy utilizing 3-maleimido proxyl (5-MSL). Most analyses are conducted on empirical levels with robust strategies that allow for the detection of dynamic changes of ligand, as well as protein. Special emphasis has been placed on the EPR spectroscopic detection of a molten globule (MG) state of HSA that is typically found by the fluorescent probe 8-Anilino- naphthalene-1-sulfonic acid (ANS). Moreover, four-pulse double electron-electron resonance (DEER) experiments are conducted and substantiated with dynamic light scattering (DLS) data to determine changes in the solution shape of HSA with pH. All results are ultimately combined in a detailed scheme that describes the pH-induced functional phase space of HSA.
Novel Enzyme Actions for Sulphated Galactofucan Depolymerisation and a New Engineering Strategy for Molecular Stabilisation of Fucoidan Degrading Enzymes
Authors: Cao Hang T. T. , Mikkelsen Maria D. , Lezyk Mateusz J. , Bui Ly M. , Tran Van T. T. , Silchenko Artem S. , Kusaykin Mikhail I. , Pham Thinh D. , Truong Bang H. , Holck Jesper , Meyer Anne S.
Published: November 2018

Fucoidans from brown macroalgae have beneficial biomedical properties but their use as pharma products requires homogenous oligomeric products. In this study, the action of five recombinant microbial fucoidan degrading enzymes were evaluated on fucoidans from brown macroalgae: Sargassum mcclurei, Fucus evanescens, Fucus vesiculosus, Turbinaria ornata, Saccharina cichorioides, and Undaria pinnatifida. The enzymes included three endo-fucoidanases (EC 3.2.1.-GH 107), FcnA2, Fda1, and Fda2, and two unclassified endo-fucoglucuronomannan lyases, FdlA and FdlB. The oligosaccharide product profiles were assessed by carbohydrate-polyacrylamide gel electrophoresis and size exclusion chromatography. The recombinant enzymes FcnA2, Fda1, and Fda2 were unstable but were stabilised by truncation of the C-terminal end (removing up to 40% of the enzyme sequence). All five enzymes catalysed degradation of fucoidans containing α(1→4)-linked l-fucosyls. Fda2 also degraded S. cichorioides and U. pinnatifida fucoidans that have α(1→3)-linked l-fucosyls in their backbone. In the stabilised form, Fda1 also cleaved α(1→3) bonds. For the first time, we also show that several enzymes catalyse degradation of S. mcclurei galactofucan-fucoidan, known to contain α(1→4) and α(1→3) linked l-fucosyls and galactosyl-β(1→3) bonds in the backbone. These data enhance our understanding of fucoidan degrading enzymes and their substrate preferences and may assist development of enzyme-assisted production of defined fuco-oligosaccharides from fucoidan substrates.
New 9α-Hydroxy-5α,6α-epoxysterols from the Vietnamese Marine Sponge Ircinia echinata
Authors: Trinh Thi , Truong Bich , Longeon Arlette , Doan Thi , Deville Alexandre , Chau Van , Pham Van , Bourguet-Kondracki Marie-Lise
Published: November 2018

Chemical investigation of the methanol extract of the Vietnamese marine sponge Ircinia echinata led to the isolation of six new 9α-hydroxy-5α,6α-epoxysterols: 5α,6α-epoxycholesta-7,22(E)-dien-3β,9α-diol (1), 5α,6α-epoxycholesta-7,24(28)-dien-3β,9α-diol (2), (24R)-5α,6α-epoxy-24-ethyl-cholesta-7-en-3β,9α-diol (3), 5α,6α-epoxycholesta-7-en-3β,9α-diol (4), (24S)-5α,6α-epoxyergosta-7,22-dien-3β,9α-diol (5), and (24R)-5α,6α-epoxy-24-methyl-cholesta-7-en-3β,9α-diol (6) along with the known 5α-6α-epoxysterols: 5α,6α-epoxystigmasta-7-en-3β-ol (7), 5α,6α-epoxystigmasta-7,22-dien-3β-ol (8), and 5α,6α-epoxyergosta-7-en-3β-ol (9). Their structures and their configurations were established on the basis of high resolution mass spectra and extensive 1D and 2D NMR spectroscopic data and by comparison with the literature. Their cytotoxic activity, evaluated against three human cancer cell lines, MCF-7, Hep-G2 and LU-1, revealed that only compounds 3 and 4 exhibited significant antiproliferative activity and compound 3 showed a selective inhibition towards the MCF-7 human breast cancer cells.
Oriented Carbon Nanostructures by Plasma Processing: Recent Advances and Future Challenges
Authors: Santhosh Neelakandan , Filipič Gregor , Tatarova Elena , Baranov Oleg , Kondo Hiroki , Sekine Makoto , Hori Masaru , Ostrikov Kostya , Cvelbar Uroš
Published: November 2018

Carbon, one of the most abundant materials, is very attractive for many applications because it exists in a variety of forms based on dimensions, such as zero-dimensional (0D), one-dimensional (1D), two-dimensional (2D), and-three dimensional (3D). Carbon nanowall (CNW) is a vertically-oriented 2D form of a graphene-like structure with open boundaries, sharp edges, nonstacking morphology, large interlayer spacing, and a huge surface area. Plasma-enhanced chemical vapor deposition (PECVD) is widely used for the large-scale synthesis and functionalization of carbon nanowalls (CNWs) with different types of plasma activation. Plasma-enhanced techniques open up possibilities to improve the structure and morphology of CNWs by controlling the plasma discharge parameters. Plasma-assisted surface treatment on CNWs improves their stability against structural degradation and surface chemistry with enhanced electrical and chemical properties. These advantages broaden the applications of CNWs in electrochemical energy storage devices, catalysis, and electronic devices and sensing devices to extremely thin black body coatings. However, the controlled growth of CNWs for specific applications remains a challenge. In these aspects, this review discusses the growth of CNWs using different plasma activation, the influence of various plasma-discharge parameters, and plasma-assisted surface treatment techniques for tailoring the properties of CNWs. The challenges and possibilities of CNW-related research are also discussed.
A New Concept on High-Calcium Flotation Wastewater Reuse
Authors: Wan He , Qu Juanping , He Tingshu , Bu Xianzhong , Yang Wei , Li Hui
Published: November 2018

Calcium ions are a kind of unavoidable ions in water. It has the deleterious effect on molybdenite flotation. High-calcium flotation wastewater (HCFW) was reused for flotation circuits after the pretreatment removing Ca2+ in from HCFW. The high cost of wastewater treatment limits HCFW reuse. In this paper, an efficient, innovative, low-cost and environmental-friendly flotation wastewater reuse technology was introduced. XLM, as a composite collector for molybdenite, is a mixture of diesel oil (DO) and polycyclic aromatic hydrocarbons (PAHs). It could reduce the deleterious effects of Ca2+ on the flotation of molybdenite in HCFW. Therefore, this was used to replace the pretreatment removing Ca2+ in from high Ca2+ wastewater and saved the cost of wastewater treatment. When XLM consists of 4 wt % PAHs and 96 wt % DO, it has better adaptability than DO in the different Ca2+ concentration of flotation water. The contact angle measurements indicated that PAHs, as a synergistic component of a composite collector, could adsorb on the edges of molybdenite in the presence of Ca2+ by forming PAHs-Ca2+-MoO42− structure to increase the contact angle of fine molybdenite particle and reduce the deleterious effects of Ca2+ on the flotation of molybdenite. The industrial-scale test further that demonstrated XLM can improve the molybdenite roughing recovery and grade by 1.8% and 3.46% compared with DO as the collector in high Ca2+ flotation wastewater. It is feasible and effective to replace high-cost wastewater treatment for molybdenum plants.
Curcumin–Copper Complex Nanoparticles for the Management of Triple-Negative Breast Cancer
Authors: Greish Khaled , Pittalà Valeria , Taurin Sebastien , Taha Safa , Bahman Fatemah , Mathur Aanchal , Jasim Anfal , Mohammed Fatima , El-Deeb Ibrahim M. , Fredericks Salim , Rashid-Doubell Fiza
Published: November 2018

Breast cancer is the most common cancer diagnosed among females worldwide. Although breast cancer survival has largely improved in the past 30 years, it remains highly heterogeneous in its response to treatment. Triple-negative breast cancer (TNBC) is a subtype of breast cancer that lacks the expression of the estrogen receptor (ER), progesterone receptor (PR) and epidermal growth factor receptor-2 (Her2). While TNBC may initially be responsive to chemotherapy, recurrence and subsequent high mortality rates are frequently reported. Studies have shown curcumin and its derivatives to be effective against TNBC cell lines in vitro. To improve its anti-cancer effects, we have synthesized Fe3+–curcumin (Fe–Cur3) and Cu2+–curcumin (CD) complexes and investigated them experimentally. Further, CD was encapsulated into a poly(styrene)-co-maleic acid (SMA) micelle to enhance its stability. We assessed the cytotoxicity of these formulations both in vitro and in vivo. SMA–CD demonstrated dose-dependent cytotoxicity and abolished TNBC tumor growth in vivo. The encapsulation of the curcumin–copper complex improved its anti-cancer activity without overt adverse effects in a murine model of TNBC. These results provide evidence and insights into the value of nanoformulations in enhancing drug-delivery and increasing the potential therapeutic efficacy of curcumin derivatives.
Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
Authors: Blomgren Jakob , Ahrentorp Fredrik , Ilver Dag , Jonasson Christian , Sepehri Sobhan , Kalaboukhov Alexei , Winkler Dag , Zardán Gómez de la Torre Teresa , Strømme Maria , Johansson Christer
Published: November 2018

We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rolling circle amplified DNA coils is injected into a tube by a peristaltic pump. The sample is moved as a plug to the two well-balanced detection coils and the dynamic magnetic moment in each position is read over a range of excitation frequencies. The time for making a complete frequency sweep over the relaxation peak is about 5 minutes (10 Hz–10 kHz with 20 data points). The obtained standard deviation of the magnetic signal at the relaxation frequency (around 100 Hz) is equal to about 10−5 (volume susceptibility SI units), which is in the same range obtained with the DynoMag system. The limit of detection with this method is found to be in the range of 1 pM.
Wavelength-Flattened Directional Coupler Based Mid-Infrared Chemical Sensor Using Bragg Wavelength in Subwavelength Grating Structure
Authors: Dong Bowei , Hu Ting , Luo Xianshu , Chang Yuhua , Guo Xin , Wang Hong , Kwong Dim-Lee , Lo Guo-Qiang , Lee Chengkuo
Published: November 2018

In this paper, we report a compact wavelength-flattened directional coupler (WFDC) based chemical sensor featuring an incorporated subwavelength grating (SWG) structure for the mid-infrared (MIR). By incorporating a SWG structure into directional coupler (DC), the dispersion in DC can be engineered to allow broadband operation which is advantageous to extract spectroscopic information for MIR sensing analysis. Meanwhile, the Bragg reflection introduced by the SWG structure produces a sharp trough at the Bragg wavelength. This sharp trough is sensitive to the surrounding refractive index (RI) change caused by the existence of analytes. Therefore, high sensitivity can be achieved in a small footprint. Around fivefold enhancement in the operation bandwidth compared to conventional DC is achieved for 100% coupling efficiency in a 40 µm long WFDC experimentally. Detection of dichloromethane (CH2Cl2) in ethanol (C2H5OH) is investigated in a SWG-based WFDC sensor 136.8 µm long. Sensing performance is studied by 3D finite-difference time domain (FDTD) simulation while sensitivity is derived by computation. Both RI sensing and absorption sensing are examined. RI sensing reveals a sensitivity of −0.47% self-normalized transmitted power change per percentage of CH2Cl2 concentration while 0.12% change in the normalized total integrated output power is realized in the absorption sensing. As the first demonstration of the DC based sensor in the MIR, our device has the potential for tertiary mixture sensing by utilizing both changes in the real and imaginary part of RI. It can also be used as a broadband building block for MIR application such as spectroscopic sensing system.
3C-SiC Nanowires In-Situ Modified Carbon/Carbon Composites and Their Effect on Mechanical and Thermal Properties
Authors: Lin Hongjiao , Li Hejun , Shen Qingliang , Shi Xiaohong , Feng Tao , Guo Lingjun
Published: November 2018

An in-situ, catalyst-free method for synthesizing 3C-SiC ceramic nanowires (SiCNWs) inside carbon–carbon (C/C) composites was successfully achieved. Obtained samples in different stages were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and Raman scattering spectroscopy. Results demonstrated that the combination of sol-gel impregnation and carbothermal reduction was an efficient method for in-situ SiCNW synthesis, inside C/C composites. Thermal properties and mechanical behaviors—including out-of-plane and in-plane compressive strengths, as well as interlaminar shear strength (ILLS) of SiCNW modified C/C composites—were investigated. By introducing SiCNWs, the initial oxidation temperature of C/C was increased remarkably. Meanwhile, out-of-plane and in-plane compressive strengths, as well as interlaminar shear strength (ILLS) of C/C composites were increased by 249.3%, 109.2%, and 190.0%, respectively. This significant improvement resulted from simultaneous reinforcement between the fiber/matrix (F/M) and matrix/matrix (M/M) interfaces, based on analysis of the fracture mechanism.
Diet Quality for Sodium and Vegetables Mediate Effects of Whole Food Diets on 8-Week Changes in Stress Load
Authors: Soltani Hoda , Keim Nancy L. , Laugero Kevin D.
Published: November 2018

Very little is known about how whole food diets, such as those based on the Dietary Guidelines for Americans (DGA), influence psychological stress and physiological stress load. To better understand the effects of whole food diets on stress, we examined in a randomized control trial the effects of a DGA-based diet on markers of psychological and physiological stress. A randomized, double-blind, controlled 8-week intervention was conducted in overweight and obese women randomly assigned to one of two diet groups: a diet based on the 2010 DGA or a diet based on a Typical American Diet (TAD). The Perceived Stress Scale and allostatic load were used to assess stress load. Eight-week change in perceived stress did not significantly (p = 0.45) differ between the DGA (+0.53 ± 0.99) and TAD (−0.57 ± 0.99) groups. Likewise, 8-week change in allostatic load did not significantly (p = 0.79) differ between the two diet intervention groups (DGA: +0.001 ± 0.26 vs. TAD: +0.105 ± 0.28). However, we did find strong inverse associations between 8-week change in stress and intervention-based improvements in diet quality (lower sodium and higher vegetable consumption). When statistically accounting for these inverse associations, we found that perceived stress and allostatic load were higher (p < 0.04) in the DGA group. These findings suggest that improvements in dietary vegetable and sodium intake mediated effects of the diet intervention on psychological and physiological stress load. That is, adopting and adhering to a diet of higher quality (DGA) for 8 weeks may have been generally more stressful in the absence of improvements in vegetable or sodium consumption. This study provides further evidence for the mental health benefits of maximizing vegetable and minimizing sodium consumption.
Low Salivary Amylase Gene (AMY1) Copy Number Is Associated with Obesity and Gut Prevotella Abundance in Mexican Children and Adults
Authors: León-Mimila Paola , Villamil-Ramírez Hugo , López-Contreras Blanca E. , Morán-Ramos Sofía , Macias-Kauffer Luis R. , Acuña-Alonzo Víctor , Del Río-Navarro Blanca E. , Salmerón Jorge , Velazquez-Cruz Rafael , Villarreal-Molina Teresa , Aguilar-Salinas Carlos A. , Canizales-Quinteros Samuel
Published: November 2018

Genome-wide association studies (GWAS) have identified copy number variants (CNVs) associated with obesity in chromosomal regions 1p31.1, 10q11.22, 11q11, 16p12.3, and recently 1p21.1, which contains the salivary amylase gene (AMY1). Recent evidence suggests this enzyme may influence gut microbiota composition through carbohydrate (mainly starch) degradation. The role of these CNVs in obesity has been scarcely explored in the Latino population, and thus the aim of our study was to evaluate the association of 1p31.1, 10q11.22, 11q11, 16p12.3 and 1p21.1 CNVs with obesity in 921 Mexican children, to replicate significant associations in 920 Mexican adults, and to analyze the association of AMY1 copy number with gut microbiota in 75 children and 45 adults. Of the five CNVs analyzed, 1q11 CNV was significantly associated with obesity in children, but not in adults. Only AMY1 CNV was significantly associated with obesity in both age groups. Moreover, gut microbiota analyses revealed a positive correlation between AMY1 copy number and Prevotella abundance. This genus has enzymes and gene clusters essential for complex polysaccharide degradation and utilization. To our knowledge, this is the first study to analyze the association of these five CNVs in the Mexican population and to report a correlation between AMY1 CN and gut microbiota in humans.
Complex Polymeric Architectures Self-Assembling in Unimolecular Micelles: Preparation, Characterization and Drug Nanoencapsulation
Authors: Ordanini Stefania , Cellesi Francesco
Published: November 2018

Unimolecular polymeric micelles are a class of single-molecule amphiphilic core-shell polymeric architectures, where the hydrophobic core is well stabilized by the hydrophilic shell, avoiding intermolecular core-core interactions. Multi-arm copolymers with a dendritic core, as well as hyperbranched and comb-like polymers, can form unimolecular micelles easily. In this review, examples of polymers able to form detectable unimolecular micelles will be presented, summarizing the analytical techniques used to characterize the unimolecular micelles and discriminate them from other supramolecular aggregates, such as multi-micelle aggregates. Unimolecular micelles are suitable for the nanoencapsulation of guest molecules. Compared to traditional supramolecular micelles, unimolecular micelles do not disassemble under dilution and are stable to environmental modifications. Recent examples of their application as drug delivery systems, endowed with increased stability and transport properties, will be discussed.
Prospects and Challenges of Phospholipid-Based Prodrugs
Authors: Markovic Milica , Ben-Shabat Shimon , Keinan Shahar , Aponick Aaron , Zimmermann Ellen M. , Dahan Arik
Published: November 2018

Nowadays, the prodrug approach is used already at the early stages of drug development. Lipidic prodrug approach is a growing field for improving a number of drug properties/delivery/therapy aspects, and can offer solutions for various unmet needs. This approach includes drug moiety bound to the lipid carrier, which can be triglyceride, fatty acids, steroid, or phospholipid (PL). The focus of this article is PL-based prodrugs, which includes a PL carrier covalently bound to the active drug moiety. An overview of relevant physiological lipid processing pathways and absorption barriers is provided, followed by drug delivery/therapeutic application of PL-drug conjugates, as well as computational modeling techniques, and a modern bioinformatics tool that can aid in the optimization of PL conjugates. PL-based prodrugs have increased lipophilicity comparing to the parent drug, and can therefore significantly improve the pharmacokinetic profile and overall bioavailability of the parent drug, join the endogenous lipid processing pathways and therefore accomplish drug targeting, e.g., by lymphatic transport, drug release at specific target site(s), or passing the blood-brain barrier. Moreover, an exciting gateway for treating inflammatory diseases and cancer is presented, by utilizing the PL sn-2 position in the prodrug design, aiming for PLA2-mediated activation. Overall, a PL-based prodrug approach shows great potential in improving different drug delivery/therapy aspects, and is expected to grow.
Universal Applicator for Digitally-Controlled Pressing Force and Impact Velocity Insertion of Microneedles into Skin
Authors: Leone Mara , Van Oorschot Bart H. , Nejadnik M. Reza , Bocchino Andrea , Rosato Matteo , Kersten Gideon , O’Mahony Conor , Bouwstra Joke , Van der Maaden Koen
Published: November 2018

Microneedle technologies have been developed for dermal drug and vaccine delivery, including hollow-, solid-, coated-, and dissolving microneedles. Microneedles have been made in many different geometries and of many different materials, all of which may influence their skin-penetrating ability. To ensure reproducible and effective drug and vaccine delivery via microneedles, the optimal insertion parameters should be known. Therefore, a digitally-controlled microneedle applicator was developed to insert microneedles into the skin via impact insertion (velocity) or via pressing force insertion. Six microneedle arrays with different geometries and/or materials were applied onto ex vivo human skin with varying velocities or pressing forces. Penetration efficiency and delivered antigen dose into the skin after application of microneedles were determined. In general, microneedles pierced the skin more efficiently when applied by impact application as compared to application via pressing force. However, the angle of application of the applicator on the skin can affect the velocity of the impact, influencing the penetration efficiency of microneedles. Regarding the antigen delivery into the skin, the delivered dose was increasing by increasing the velocity or pressure, and thus, increasing the penetration efficiency. These data demonstrate that an applicator is an important tool to determine optimal application conditions with ex vivo human skin.
Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress
Authors: Pintus Eliana , Kadlec Martin , Jovičić Marija , Sedmíková Markéta , Ros-Santaella José Luis
Published: November 2018

Aminoguanidine is a selective inhibitor of the inducible nitric oxide synthase (iNOS) and a scavenger of reactive oxygen species (ROS). Numerous studies have shown the antioxidant properties of aminoguanidine in several cell lines, but the in vitro effects of this compound on spermatozoa under oxidative stress are unknown. In this study, we tested the hypothesis that aminoguanidine may protect against the detrimental effects of oxidative stress in boar spermatozoa. For this purpose, sperm samples were incubated with a ROS generating system (Fe2+/ascorbate) with or without aminoguanidine supplementation (10, 1, and 0.1 mM). Our results show that aminoguanidine has powerful antioxidant capacity and protects boar spermatozoa against the deleterious effects of oxidative stress. After 2 h and 3.5 h of sperm incubation, the samples treated with aminoguanidine showed a significant increase in sperm velocity, plasma membrane and acrosome integrity together with a reduced lipid peroxidation in comparison with control samples (p < 0.001). Interestingly, except for the levels of malondialdehyde, the samples treated with 1 mM aminoguanidine did not differ or showed better performance than control samples without Fe2+/ascorbate. The results from this study provide new insights into the application of aminoguanidine as an in vitro therapeutic agent against the detrimental effects of oxidative stress in semen samples.
Elemental Profiles in Cycas micronesica Stems
Authors: Marler Thomas E.
Published: November 2018

Essential nutrients and metals have been quantified in stems of many tree species to understand the role of stems as storage and source organs. Little is known about stored stem resources of cycad tree species. Cycas micronesica tissue was collected from apical and basal axial regions of stems; and pith, vascular, and cortex tissues were separated into three radial regions. Leaves were also sampled to provide a comparison to stems. Minerals and metals were quantified in all tissues. Minerals and metals varied greatly among the six stem sections. Phosphorus varied more among the three radial sections than the other macronutrients, and zinc and nickel varied more than the other micronutrients. Stem carbon was less than and stem calcium was greater than expected, based on what is currently known tree stem concentrations in the literature. Elemental concentrations were generally greater than those previously reported for coniferous gymnosperm trees. Moreover, the stem concentrations were high in relation to leaf concentrations, when compared to published angiosperm and conifer data. The results indicated that the addition of more cycad species to the literature will improve our understanding of gymnosperm versus angiosperm stem nutrient relations, and that the non-woody cycad stem contains copious essential plant nutrients that can be mobilized and deployed to sinks when needed.