bluuebunny/crossref_metadata_2025 huggingface dataset:
KEAP1/NFE2L2 Mutations of Liquid Biopsy as Prognostic Biomarkers in Patients With Advanced Non-Small Cell Lung Cancer: Results From Two Multicenter, Randomized Clinical Trials
Authors: Zhu Hongyuan , Xie Daipeng , Yu Yunfang , Yao Lintong , Xu Bin , Huang Luyu , Wu Shaowei , Li Fasheng , Zheng Yating , Liu Xinyi , Xie Wenzhuan , Huang Mengli , Li Hao , Zheng Shaopeng , Zhang Dongkun , Qiao Guibin , Chan Lawrence W. C. , Zhou Haiyu
Published: July 2021

Purpose

The KEAP1-NFE2L2 (Kelch-like ECH-associated protein 1 (KEAP1)-Nuclear factor (erythroid-derived 2)-like 2 (NFE2L2)) mutations are associated with resistance to chemotherapy or immunotherapy in non-small cell lung cancer (NSCLC). Conversely, it has been reported that NFE2L2 mutations potentiate improved clinical outcome with immunotherapy. However, therapeutic benefits for patients with KEAP1/NFE2L2 mutations remain unclear. The purpose of this study was to investigate the association between KEAP1/NFE2L2 and NSCLC prognosis, and to explore whether immunotherapy can improve prognosis in populations with KEAP1/NFE2L2 mutations.

Experimental Design

The impact of KEAP1/NFE2L2 mutations on survival outcomes in NSCLC patients received immunotherapy and chemotherapy was verified in the randomized phase II/III POPLAR/OAK trials (blood-based sequencing, bNGS cohort, POPLAR (n = 211) and OAK (n = 642)). The Cancer Genome Atlas (TCGA) NSCLC cohort (n=998) and an in-house Chinese NSCLC cohort (n=733) was used For the analysis of immune-related markers.

Results

Compared with KEAP1/NFE2L2 wild-type, patients with KEAP1/NFE2L2 mutations were significantly associated with poorer overall survival (OS, HR = 1.97, 95% CI 1.48–2.63, P < 0.001) on atezolizumab and docetaxel (HR = 1.66, 95% CI 1.28–2.16, P < 0.001). In KEAP1/NFE2L2 mutant group, there was no significant difference in median OS between atezolizumab and docetaxel (HR 0.74, 95% CI 0.53–1.03, P = 0.07). NFE2L2/KEAP1 mutations were significantly associated with higher TMB values and PD-L1 expression in the OAK/POPLAR and in-house Chinese NSCLC cohorts. GSEA revealed that KEAP1/NFE2L2mutant subgroup was associated with deficient infiltration of CD4+ T cells, NK T cells and natural Treg cells, and lower expression of DNA damage response genes in TCGA NSCLC cohort.

Conclusions

Our study revealed that patients with KEAP1/NFE2L2 mutations have a worse prognosis than wild-type patients, both on immunotherapy and chemotherapy. In addition, in patients with KEAP1/NFE2L2 mutations, immunotherapy did not significantly improve prognosis compared to chemotherapy.
SIRT1/PGC-1α/PPAR-γ Correlate With Hypoxia-Induced Chemoresistance in Non-Small Cell Lung Cancer
Authors: Xu Rui , Luo Xin , Ye Xuan , Li Huan , Liu Hongyue , Du Qiong , Zhai Qing
Published: July 2021

Resistance is the major cause of treatment failure and disease progression in non-small cell lung cancer (NSCLC). There is evidence that hypoxia is a key microenvironmental stress associated with resistance to cisplatin, epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), and immunotherapy in solid NSCLCs. Numerous studies have contributed to delineating the mechanisms underlying drug resistance in NSCLC; nevertheless, the mechanisms involved in the resistance associated with hypoxia-induced molecular metabolic adaptations in the microenvironment of NSCLC remain unclear. Studies have highlighted the importance of posttranslational regulation of molecular mediators in the control of mitochondrial function in response to hypoxia-induced metabolic adaptations. Hypoxia can upregulate the expression of sirtuin 1 (SIRT1) in a hypoxia-inducible factor (HIF)-dependent manner. SIRT1 is a stress-dependent metabolic sensor that can deacetylate some key transcriptional factors in both metabolism dependent and independent metabolic pathways such as HIF-1α, peroxisome proliferator-activated receptor gamma (PPAR-γ), and PPAR-gamma coactivator 1-alpha (PGC-1α) to affect mitochondrial function and biogenesis, which has a role in hypoxia-induced chemoresistance in NSCLC. Moreover, SIRT1 and HIF-1α can regulate both innate and adaptive immune responses through metabolism-dependent and -independent ways. The objective of this review is to delineate a possible SIRT1/PGC-1α/PPAR-γ signaling-related molecular metabolic mechanism underlying hypoxia-induced chemotherapy resistance in the NSCLC microenvironment. Targeting hypoxia-related metabolic adaptation may be an attractive therapeutic strategy for overcoming chemoresistance in NSCLC.
International Survey on the Use of Arginine Vasopressin in the Postoperative Management of Single Ventricle Patients
Authors: Raghavan Vidya R. , da Cruz Eduardo M. , Kaufman Jon , Osorio Lujan Suzanne
Published: July 2021

Management of patients with single ventricle physiology after surgical palliation is challenging. Arginine vasopressin has gained popularity in recent years as a non-catecholamine vasoactive medication due to its unique properties. However, data regarding its use in the pediatric population is limited. Therefore, we designed a survey to explore whether and how clinicians use this medication in intensive care units for the postoperative management of single ventricle patients. This international survey aimed to assess usage, practices, and concepts related to arginine vasopressin in pediatric intensive care units worldwide. Directors of pediatric intensive care units who are members of the following international professional societies: European Society of Pediatric Neonatal Intensive Care, Association for European Pediatric and Congenital Cardiology, and Pediatric Cardiac Intensive Care Society were invited to participate in this survey. Of the 62 intensive care unit directors who responded, nearly half use arginine vasopressin in the postoperative management of neonatal single ventricle patients, and 90% also use the drug in subsequent surgical palliation. The primary indications are vasoplegia, hemodynamic instability, and refractory shock, although it is still considered a second-line medication. Conceptual benefits include improved hemodynamics and end-organ perfusion and decreased incidence of low cardiac output syndrome. Those practitioners who do not use arginine vasopressin cite lack of availability, fear of potential adverse effects, unclear indication for use, and lack of evidence suggesting improved outcomes. Both users and non-users described increased myocardial afterload and extreme vasoconstriction as potential disadvantages of the medication. Despite the lack of conclusive data demonstrating enhanced clinical outcomes, our study found arginine vasopressin is used widely in the care of infants and children with single ventricle physiology after the first stage and subsequent palliative surgeries. While many intensive care units use this medication, few had protocols, offering an area for further growth and development.
Potential Role of Traditional Chinese Medicines by Wnt/β-Catenin Pathway Compared With Targeted Small Molecules in Colorectal Cancer Therapy
Authors: Chang Jinrong , Xavier Hoileong Wong , Chen Dongfeng , Liu Yamei , Li Hui , Bian Zhaoxiang
Published: July 2021

Colorectal cancer (CRC) has become a global public health problem because of its high incidence and mortality rate worldwide. The previous clinical treatment for CRC mainly involves conventional surgery, chemotherapy, and radiotherapy. With the development of tumor molecular targeted therapy, small molecule inhibitors present a great advantage in improving the survival of patients with advanced CRC. However, various side effects and drug resistance induced by chemotherapy are still the major obstacles to improve the clinical benefit. Thus, it is crucial to find new and alternative drugs for CRC treatment. Traditional Chinese medicines (TCMs) have been proved to have low toxicity and multi-target characteristics. In the last few decades, an increasing number of studies have demonstrated that TCMs exhibit strong anticancer effects in both experimental and clinical models and may serve as alternative chemotherapy agents for CRC treatment. Notably, Wnt/β-catenin signaling pathway plays a vital role in the initiation and progression of CRC by modulating the stability of β-catenin in the cytoplasm. Targeting Wnt/β-catenin pathway is a novel direction for developing therapies for CRC. In this review, we outlined the anti-tumor effects of small molecular inhibitors on CRC through Wnt/β-catenin pathway. More importantly, we focused on the potential role of TCMs against tumors by targeting Wnt/β-catenin signaling at different stages of CRC, including precancerous lesions, early stage of CRC and advanced CRC. Furthermore, we also discussed perspectives to develop potential new drugs from TCMs via Wnt/β-catenin pathway for the treatment of CRC.
Differential Expression of Myogenic and Calcium Signaling-Related Genes in Broilers Affected With White Striping
Authors: Marciano Caroline Michele Marinho , Ibelli Adriana Mércia Guaratini , Marchesi Jorge Augusto Petroli , de Oliveira Peixoto Jane , Fernandes Lana Teixeira , Savoldi Igor Ricardo , do Carmo Kamilla Bleil , Ledur Mônica Corrêa
Published: July 2021

White Striping (WS) has been one of the main issues in poultry production in the last years since it affects meat quality. Studies have been conducted to understand WS and other myopathies in chickens, and some biological pathways have been associated to the prevalence of these conditions, such as extracellular calcium level, oxidative stress, localized hypoxia, possible fiber-type switching, and cellular repairing. Therefore, to understand the genetic mechanisms involved in WS, 15 functional candidate genes were chosen to be analyzed by quantitative PCR (qPCR) in breast muscle of normal and WS-affected chickens. To this, the pectoral major muscle (PMM) of 16 normal and 16 WS-affected broilers were collected at 42 days of age and submitted to qRT-PCR analysis. Out of the 15 genes studied, six were differentially expressed between groups. The CA2, CSRP3, and PLIN1 were upregulated, while CALM2, DNASE1L3, and MYLK2 genes were downregulated in the WS-affected when compared to the normal broilers. These findings highlight that the disruption on muscle and calcium signaling pathways can possibly be triggering WS in chickens. Improving our understanding on the genetic basis involved with this myopathy might contribute for reducing WS in poultry production.
Effect of Humic Acid Addition on Buffering Capacity and Nutrient Storage Capacity of Soilless Substrates
Authors: Xu Jingcheng , Mohamed Esraa , Li Qiang , Lu Tao , Yu Hongjun , Jiang Weijie
Published: July 2021

Excessive application of fertilizers has become a major issue in croplands of intensive agricultural systems in China, resulting in severe non-point source pollution; thus, reduction in the use of chemical fertilizers has received significant attention. Improving the nutrient storage capacity of soils or substrates is an effective approach for solving this problem. Humic acids (HA) are excellent soil conditioners. Thus, in the present study, their ability to improve the physico-chemical properties of three substrates with different textures was evaluated. HA treatments included 1% HA root application in three different types of substrates, including pure sand, pure cocopeat, and a mixture of sand:cocopeat (1:1, v/v) and their relative controls. We examined the morphological parameters of cucumber seedlings as well as pH buffering capacity (pHBC), total organic carbon (TOC), organic matter (OM), cation exchange capacity (CEC), and nutrient storage capacity of the three substrates. The results show that HA application improved the morphological parameters of cucumber seedlings (plant height, stem diameter, and biomass) in pure cocopeat and cocopeat-sand mixture treatments. On the contrary, HA addition had harmful effects on the cucumber seedlings cultivated in sand due to the low pHBC of sand. The seedlings cultivated in pure cocopeat showed the best morphological parameter performances among the seedlings grown in the three substrates. Furthermore, pHBC, TOC, OM, and CEC were enhanced by HA application. Incorporation of HA improved ammonium (NH4+) and potassium (K+) storage capacity while decreasing phosphorus (P) storage. Pure cocopeat had the highest pHBC, TOC, OM, CEC, and nutrient storage capacity among the three substrates. In conclusion, mixing 1% HA into substrates promoted cucumber growth, improved substrate properties, and enhanced fertilizer use efficiency. Pure cocopeat is a suitable substrate for cucumber cultivation, and mixing cocopeat with sand amends the substrate properties and consequently improves plant growth.
The Arabidopsis 2′-O-Ribose-Methylation and Pseudouridylation Landscape of rRNA in Comparison to Human and Yeast
Authors: Streit Deniz , Schleiff Enrico
Published: July 2021

Eukaryotic ribosome assembly starts in the nucleolus, where the ribosomal DNA (rDNA) is transcribed into the 35S pre-ribosomal RNA (pre-rRNA). More than two-hundred ribosome biogenesis factors (RBFs) and more than two-hundred small nucleolar RNAs (snoRNA) catalyze the processing, folding and modification of the rRNA in Arabidopsis thaliana. The initial pre-ribosomal 90S complex is formed already during transcription by association of ribosomal proteins (RPs) and RBFs. In addition, small nucleolar ribonucleoprotein particles (snoRNPs) composed of snoRNAs and RBFs catalyze the two major rRNA modification types, 2′-O-ribose-methylation and pseudouridylation. Besides these two modifications, rRNAs can also undergo base methylations and acetylation. However, the latter two modifications have not yet been systematically explored in plants. The snoRNAs of these snoRNPs serve as targeting factors to direct modifications to specific rRNA regions by antisense elements. Today, hundreds of different sites of modifications in the rRNA have been described for eukaryotic ribosomes in general. While our understanding of the general process of ribosome biogenesis has advanced rapidly, the diversities appearing during plant ribosome biogenesis is beginning to emerge. Today, more than two-hundred RBFs were identified by bioinformatics or biochemical approaches, including several plant specific factors. Similarly, more than two hundred snoRNA were predicted based on RNA sequencing experiments. Here, we discuss the predicted and verified rRNA modification sites and the corresponding identified snoRNAs on the example of the model plant Arabidopsis thaliana. Our summary uncovers the plant modification sites in comparison to the human and yeast modification sites.
EgmiR5179 Regulates Lipid Metabolism by Targeting EgMADS16 in the Mesocarp of Oil Palm (Elaeis guineensis)
Authors: Wang Yifei , Zou Jixin , Zhao Jin , Zheng Yusheng , Li Dongdong
Published: July 2021

EgMADS16, one of the MADS-box transcription factors in oil palm, has a high expression level in the late fruit development of the oil palm fruit mesocarp. At the same time, it is also predicted to be the target gene of EgmiR5179, which has been identified in previous research. In this paper, we focused on the function and regulatory mechanism of the EgMADS16 gene in oil palm lipid metabolism. The results indicated that the transcription level of EgMADS16 was highest in the fourth stage, and a dual-luciferase reporter assay proved that the EgMADS16 expression level was downregulated by EgmiR5179. In both the OXEgMADS16 Arabidopsis seeds and oil palm embryonic calli, the total lipid contents were significantly decreased, but the contents of C18:0 and C18:3 in OXEgMADS16 lines were significantly increased. As expected, EgmiR5179 weakened the inhibitory effect of EgMADS16 on the oil contents in transgenic Arabidopsis plants that coexpressed EgmiR5179 and EgMADS16 (OXEgmiR5179-EgMADS16). Moreover, yeast two-hybrid and BiFC analyses suggested that there was an interaction between the EgMADS16 protein and EgGLO1 protein, which had been proven to be capable of regulating fatty acid synthesis in our previous research work. In summary, a model of the molecular mechanism by which miRNA5179 targets EgMADS16 to regulate oil biosynthesis was hypothesized, and the research results provide new insight into lipid accumulation and molecular regulation in oil palm.
Feeling Heard: Experiences of Listening (or Not) at Work
Authors: Kriz Tiffany D. , Kluger Avraham N. , Lyddy Christopher J.
Published: July 2021

Listening has been identified as a key workplace skill, important for ensuring high-quality communication, building relationships, and motivating employees. However, recent research has increasingly suggested that speaker perceptions of good listening do not necessarily align with researcher or listener conceptions of good listening. While many of the benefits of workplace listening rely on employees feeling heard, little is known about what constitutes this subjective perception. To better understand what leaves employees feeling heard or unheard, we conducted 41 interviews with bank employees, who collectively provided 81 stories about listening interactions they had experienced at work. Whereas, prior research has typically characterized listening as something that is perceived through responsive behaviors within conversation, our findings suggest conversational behaviors alone are often insufficient to distinguish between stories of feeling heard vs. feeling unheard. Instead, our interviewees felt heard or unheard only when listeners met their subjective needs and expectations. Sometimes their needs and expectations could be fulfilled through conversation alone, and other times action was required. Notably, what would be categorized objectively as good listening during an initial conversation could be later counteracted by a failure to follow-through in ways expected by the speaker. In concert, these findings contribute to both theory and practice by clarifying how listening behaviors take on meaning from the speakers' perspective and the circumstances under which action is integral to feeling heard. Moreover, they point toward the various ways listeners can engage to help speakers feel heard in critical conversations.
The Vicious Cycle: Problematic Family Relations, Substance Abuse, and Crime in Adolescence: A Narrative Review
Authors: Saladino Valeria , Mosca Oriana , Petruccelli Filippo , Hoelzlhammer Lilli , Lauriola Marco , Verrastro Valeria , Cabras Cristina
Published: July 2021

Despite the copiousness of studies on the risky behaviors of adolescents, we cannot establish with certainty the leading aspects involved in teens’ substance abuse and criminal actions. This review aims to explore the interplay among the family system, substance abuse, and criminal behavior. An analysis of the main results of the 61 articles published between 2010 and 2020 shows that adolescents whose parents are justice-involved and often absent from home are more likely to perceive lower cohesion, support, and poor family communication. These factors can involve them in criminal acts and substance abuse. Moreover, these conducts are often linked to a form of uneasiness and a search of autonomy. Indeed, risky behaviors could have more than one meaning. Our findings also suggest that the most diffused drug-related crimes in adolescence are economic crimes, weapon carrying, robberies, dealing, and drug possession. Considering these results, future clinical implications might be based on multidimensional approaches, focusing more on the family context to promote interventions for at-risk adolescents.
Cognitive, Language, and Behavioral Outcomes in Children With Autism Spectrum Disorders Exposed to Early Comprehensive Treatment Models: A Meta-Analysis and Meta-Regression
Authors: Shi Bijun , Wu Weijia , Dai Meixia , Zeng Jingjing , Luo Jingyin , Cai Li , Wan Bin , Jing Jin
Published: July 2021

Background: Early comprehensive treatment models (CTMs) have been developed as effective treatments for children with autism spectrum disorder (ASD). Numerous studies have suggested that CTMs can improve short-term outcomes, but little is known about precise outcome information in childhood. The current meta-analysis reviewed studies reporting broader outcomes in children with ASD who had ever participated in a CTM and examined the predictors of developmental gains.

Methods: We searched eight databases up to June 13, 2019, for relevant trials and natural experiments. Longitudinal studies were selected if they investigated the outcomes of CTMs. Two meta-analyses were undertaken to provide a summary estimate of change in treatment outcomes and to evaluate the effect of CTMs; one used the standardized mean change between the pretest and posttest, and the other was a classical meta-analysis. Stratified and random-effects meta-regression analyses were performed to search for outcome differences among studies.

Results: Eighteen intervention studies (involving 495 children with ASD) met all the inclusion criteria: 12 used early intensive behavioral intervention (EIBI), and two used the Early Start Denver Model (ESDM). Outcomes were categorized into three parts: cognitive, language and behavioral (e.g., adaptive functioning and symptomatology). Overall, most children with ASD who had ever participated in an early CTM made gains in many areas of functioning, especially in terms of symptom- and language-related outcomes. Stratified analyses indicated that the ESDM displayed the largest effect on IQ improvement (ES = 1.37, 95% CI: 0.95 to 1.80), while EIBI was more effective for symptom reduction (ES = −1.27, 95% CI: −1.96 to −0.58). Further, meta-regression suggested that interventions with parent involvement, higher intensity, and longer treatment hours yielded greater improvements in IQ and social adaptive functioning, respectively.

Conclusion: The results demonstrate a positive association between CTMs and better prognosis in childhood, especially regarding symptoms, and language. However, most extant research involves small, non-randomized studies, preventing definitive conclusions from being drawn. Clearly, the outcomes of children with ASD are still far from normal, especially with respect to adaptive functioning, and the four mediating variables pertaining to treatment elements can affect their gains, including approach, implementer, intensity, and total treatment hours.

Systematic Review Registration: [www.crd.york.ac.uk/PROSPERO], identifier [CRD42019146859].
Gambling-Like Day Trading During the COVID-19 Pandemic – Need for Research on a Pandemic-Related Risk of Indebtedness and Mental Health Impact
Authors: Håkansson Anders , Fernández-Aranda Fernando , Jiménez-Murcia Susana
Published: July 2021

Stock exchange trading increasingly has been highlighted as a possible cause of gambling disorder, typically in rapid and excessive “day trading” which may cause over-indebtedness and mental health problems. The COVID-19 pandemic has been suspected to increase online gambling and gambling problems. In a number of recent media reports, day trading has been reported to increase during COVID-19, possibly in relation to changes in everyday life, financial problems and job insecurity during the pandemic. Increasing day trading has thereby been suspected to cause addictive behavior, financial difficulties, and poor mental health. However, there is hitherto a lack of research in the area. The present paper addresses the potential for day trading to cause problem gambling, debts and mental health problems, and calls for research and clinical guidelines in problem gambling related to stock market behavior as a problematic gambling behavior. Screening tools, awareness among clinicians, and longitudinal research studies may be warranted, both during the COVID-19 pandemic and beyond.
Surveillance on Healthcare Workers During the First Wave of SARS-CoV-2 Pandemic in Italy: The Experience of a Tertiary Care Pediatric Hospital
Authors: Guarnieri Valentina , Moriondo Maria , Giovannini Mattia , Lodi Lorenzo , Ricci Silvia , Pisano Laura , Barbacci Paola , Bini Costanza , Indolfi Giuseppe , Zanobini Alberto , Azzari Chiara
Published: July 2021

Healthcare workers (HCWs) play a central role in handling the ongoing coronavirus disease 2019 (COVID-19) pandemic. Monitoring HCWs, both symptomatic and asymptomatic, through screening programs, are critical to avoid the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in the hospital environment to rapidly identify and isolate infected individuals and to allow their prompt return to work as soon as necessary. We aim to describe our healthcare surveillance experience (April 2–May 6, 2020) based on a combined screening consisting of real-time PCR (RT-PCR) on nasopharyngeal (NP) swabs and rapid serologic tests (RST) for SARS-CoV-2 in all HCWs of Meyer Children's University Hospital in Florence. Among the analyzed workers, 13/1690 (0.8%), all of them without clinical manifestations, was found positive for SARS-CoV-2 by using RT-PCR on NP swab: 8/1472 (0.5%) were found positive during the screening, 1/188 (0.5%) during contact with a positive individual (p > 0.05 vs. screening group), while 4/30 (13.3%) were found positive on the day of re-admission at work after an influenza-like-illness (p < 0.05). Concerning working areas, the majority of RT-PCR positivity (12/13) and serologic positivity (34/42) was found in non-COVID-19 dedicated areas (p > 0.05 vs. COVID-19 dedicated areas). No cases were registered among non-patients-facing workers (p = 0.04 vs. patient-facing group). Nurses and residents represented, respectively, the working role with the highest and lowest percentage of RT-PCR positivity. In conclusion, accurate surveillance is essential to reduce virus spread among HCWs, patients, and the community and to limit the shortage of skilled professionals. The implementation of the surveillance system through an efficient screening program was offered to all professionals, regardless of the presence of clinical manifestations and the level of working exposure risk, maybe wise and relevant.
Association Between Perceived Stress and Post-Traumatic Stress Disorder Among Medical Staff During the COVID-19 Epidemic in Wuhan City
Authors: Mei Songli , Liang Leilei , Ren Hui , Hu Yueyang , Qin Zeying , Cao Ruilin , Li Chuanen , Fei Junsong , Yuan Tongshuang , Meng Cuicui , Guo Xinmeng , Lv Jianping , Hu Yuanchao
Published: July 2021

Objective: The study aimed to examine the relationship between perceived stress and post-traumatic stress disorder (PTSD) among frontline medical staff during the lockdown in Wuhan city, China, due to the COVID-19 outbreak.

Methods: The study was conducted in August 2020, which included 516 medical staff between 21 to 65 years. The PTSD Checklist-Civilian, Perceived Stress Scale, Insomnia Severity Index, and Compassion Fatigue Short Scale were used.

Results: The results indicated that 10.5% of the medical staff experienced PTSD symptoms, and insomnia severity mediated the effect of perceived stress on PTSD. In addition, compassion fatigue moderated the association between perceived stress and PTSD.

Conclusion: The study elucidated the mechanisms underlying the association between perceived stress and PTSD. Moreover, it emphasized the importance of long-term monitoring of the mental health status of frontline medical staff who supported Wuhan. The results can serve as reference for relevant medical and health departments to formulate active interventions and preventive measures against PTSD for unsung heroes who put their lives on the line during difficult times.
Case Report: Osteomyelitis of the Proximal Phalanx of the Finger in Patient With Ollier Disease
Authors: Lipatov Konstantin , Melkonyan George , Shekhter Anatoly , Asatryan Artur , Kholikov Abduaziz , Fayzullin Alexey
Published: July 2021

Ollier disease is a rare congenital pathology characterized by the growth of enchondromas in bones, accompanied with their deformities, fractures, and the risk of malignancy. A 39-year-old patient with Ollier disease (acroform with lesions of hands and feet) suffered a rapid development of osteomyelitis of the proximal phalanx of the ring finger after a mosquito bite. The condition localized in the area of enchondroma. Surgical treatment included osteonecrectomy in the phalanx and enchondroma with excision of non-viable surrounding soft tissues, drainage of the surgical wound and the imposition of primary sutures. Morphological analysis confirmed the presence of ectopic embryonic cartilage specific for Ollier disease and the bone destruction. The excised tissues were infiltrated with immune cells and had signs of periosteal chronic inflammation including fibrosis and hyalinosis. These changes, which occurred long before the mosquito bite, became a favorable background for the development of a purulent infection.
Drought and Saline Stress Tolerance Induced in Somatic Hybrids of Solanum chacoense and Potato Cultivars by Using Mismatch Repair Deficiency
Authors: Molnár Imola , Cozma Lavinia , Dénes Tünde-Éva , Vass Imre , Vass István-Zoltán , Rakosy-Tican Elena
Published: July 2021

Global climate change, especially when involving drought and salinity, poses a major challenge to sustainable crop production, causing severe yield losses. The environmental conditions are expected to further aggravate crop production in the future as a result of continuous greenhouse gas emissions, causing further temperature rise and leading to increased evapotranspiration, severe drought, soil salinity, as well as insect and disease threats. These suboptimal growth conditions have negative impact on plant growth, survival, and crop yield. Potato is well known as a crop extremely susceptible to drought, which is primarily attributed to its shallow root system. With potato being the fourth major food crop, increasing potato productivity is thus important for food security and for feeding global population. To maintain a sustainable potato production, it is necessary to develop stress tolerant potato cultivars that cope with the already ongoing climate change. The aim of our study is to analyze the response of potato somatic hybrids to drought and salt stress under in vitro conditions; the somatic hybrids studied are the wild relative Solanum chacoense (+) Solanum tuberosum, with or without mismatch repair deficiency (MMR). Upon this selection of drought and salt tolerant genotypes, somatic hybrids and their parents were phenotyped on a semi-automated platform, and lines tolerant to medium water scarcity (20% compared to 60% soil water capacity) were identified. Although none of the parental species were tolerant to drought, some of the MMR-deficient somatic hybrids showed tolerance to drought and salt as a new trait.
Can Long-Term Experiments Predict Real Field N and P Balance and System Sustainability? Results from Maize, Winter Wheat, and Soybean Trials Using Mineral and Organic Fertilisers
Authors: Piccoli Ilaria , Sartori Felice , Polese Riccardo , Borin Maurizio , Berti Antonio
Published: July 2021

Agri-environmental indicators such as nutrient balance may play a key role in soil and water quality monitoring, although short-term experiments might be unable to capture the sustainability of cropping systems. Therefore, the objectives of this study are: (i) to evaluate the reliability of long-term experimental N and P balance estimates to predict real field (RF) (i.e., short-term transitory) conditions; and (ii) to compare the sustainability of short- and long-term experiments. The LTE-based predictions showed that crops are generally over-fertilised in RF conditions, particularly maize. Nutrient balance predictions based on the LTE data tended to be more optimistic than those observed under RF conditions, which are often characterised by lower outputs; in particular, 13, 44, and 47% lower yields were observed for winter wheat, maize, and soybean, respectively, under organic management. The graphical evaluation of N and P use efficiency demonstrated the benefit of adopting crop rotation practices and the risk of nutrient loss when liquid organic fertiliser was applied on a long-term basis. In conclusion, LTE predictions may depend upon specific RF conditions, representing potential N and P use efficiencies that, in RF, may be reduced by crop yield-limiting factors and the specific implemented crop sequence.
Short-Term Responses to Salinity of Soybean and Chenopodium album Grown in Single and Mixed-Species Hydroponic Systems
Authors: Ghirardelli Aurora , Schiavon Michela , Zanin Giuseppe , Ostapczuk Piotr , Masin Roberta
Published: July 2021

Weeds account for losses in crop yields, and this event might be exacerbated by salinity. Therefore, we investigated the responses of Chenopodium album L. and soybean (Glycine max (L.) Merr.) to salt stress, as well as interferences between species. Ten-day old plants were grown for 1 week in a single- or mixed-species set-up, either with or without 100 mM of NaCl. C. album reduced the biomass of soybean similarly to salt stress, while its growth was unaffected under any condition. C. album decreased the crop protein content when salinity was applied. This effect was ascribed to altered protein metabolism and/or N usage to produce other N metabolites, including osmolytes. The two species did not reciprocally affect the capacity to accumulate Na+, but the weed contained two-fold more Na+ in the leaves. Elevated initial K+ concentration and high K+ delivery to the shoot likely explained the better acclimation of C. album to salinity. C. album produced more phenolics and proline and exhibited greater antioxidant activity, but low lipid peroxidation, in the mixed set-up under salinity. Thus, it is possible that the weed could become more resilient to salinity when growing in a soybean field. In the long term, this might cause significant losses in soybean productivity as expected by the dramatic decline in crop protein content.
Radiogrametric Analysis of the Thoracic Limb Phalanges in Arabian Horses and Thoroughbred Horses
Authors: Gündemir Ozan , Szara Tomasz , Pazvant Gülsün , Erdikmen Dilek Olğun , Duro Sokol , Perez William
Published: July 2021

In this study, it was aimed to determine the statistical differences between Arabian horses and Thoroughbred horses based on X-ray images of forelimb digital bones. Latero-medial X-ray images of digital bones of thoracic limbs were taken of 25 Arabian horses and 50 Thoroughbred healthy horses. The difference between males and females within the breed was statistically analyzed as well. Nine measurements and three indexes taken from phalanges of thoracic limbs were used. Thoroughbred horses did not differ significantly between sexes, as indicated by the ANOVA. For the Arabian horses, the length of the middle of the proximal phalanx (p < 0.05), the length of the middle of the middle phalanx (p < 0.001), and the length of the dorsal surface of the distal phalanx (p < 0.05) measurement points were found to be differentiated between sexes. In the analysis made between Thoroughbred horses and Arabian horses with no respect to sex, the critical measurement was the depth of the caput of the proximal phalanx. The discriminant analysis enabled the correct classification of 89.33% of the proximal phalanx samples to the exact breed. The correct classification rate was 77.33% in the case of middle phalanx and 54.67% in the case of distal phalanx. Measurement results of the distal phalanx were found to be insignificant between both breeds and sexes. The radiological measurements of digital bones showed that sexual dimorphism was not too expressed and that decisive differences were found between the breeds.
Evaluation of Multi-Sensor Fusion Methods for Ultrasonic Indoor Positioning
Authors: Mannay Khaoula , Ureña Jesús , Hernández Álvaro , Villadangos José M. , Machhout Mohsen , Aguili Taoufik
Published: July 2021

Indoor positioning systems have become a feasible solution for the current development of multiple location-based services and applications. They often consist of deploying a certain set of beacons in the environment to create a coverage volume, wherein some receivers, such as robots, drones or smart devices, can move while estimating their own position. Their final accuracy and performance mainly depend on several factors: the workspace size and its nature, the technologies involved (Wi-Fi, ultrasound, light, RF), etc. This work evaluates a 3D ultrasonic local positioning system (3D-ULPS) based on three independent ULPSs installed at specific positions to cover almost all the workspace and position mobile ultrasonic receivers in the environment. Because the proposal deals with numerous ultrasonic emitters, it is possible to determine different time differences of arrival (TDOA) between them and the receiver. In that context, the selection of a suitable fusion method to merge all this information into a final position estimate is a key aspect of the proposal. A linear Kalman filter (LKF) and an adaptive Kalman filter (AKF) are proposed in that regard for a loosely coupled approach, where the positions obtained from each ULPS are merged together. On the other hand, as a tightly coupled method, an extended Kalman filter (EKF) is also applied to merge the raw measurements from all the ULPSs into a final position estimate. Simulations and experimental tests were carried out and validated both approaches, thus providing average errors in the centimetre range for the EKF version, in contrast to errors up to the meter range from the independent (not merged) ULPSs.
Smart Cities and Data Analytics for Intelligent Transportation Systems: An Analytical Model for Scheduling Phases and Traffic Lights at Signalized Intersections
Authors: Gunes Fatih , Bayrakli Selim , Zaim Abdul Halim
Published: July 2021

This paper is intended to improve the performance of signalized intersections, one of the most important systems of traffic control explained within the scope of smart transportation systems. These structures, which have the main role in ensuring the order and flow of traffic, are alternative systems depending on the different methods and techniques used. Determining the operation principles of these systems requires an extremely careful and planned study, considering their important role. Performance outputs obtained from the queue analyses made in previous studies formed the input of this study. The most important techniques are used in the effective control of intersections, such as signal timing: in particular, the use of effective green time and order of the transitions between phases. In this research, a traffic-sensitive signalized intersection control system was designed with the suggested methods against these two problems. The sample intersections were selected from three cities with the highest population density as the case study area. In the analysis of the performance of the connection arms of the selected intersections, flow intensity data were taken into consideration, as well as the arrival and service rates. Based on this, the outputs of the two proposed models regarding the use of phase transitions and effective green durations were compared with two other adaptive control systems and their positive results were shared. The results showed that signalized intersections, operating with a well-planned and correctly chosen technique, better regulate density and queuing.
Improvement and Performance Evaluation When Implementing a Distance-Based Registration
Authors: Baek Jang Hyun
Published: July 2021

An efficient location registration scheme is essential to continuously accommodate the increasing number of mobile subscribers and to offer a variety of multimedia services with good quality. The objective of this study was to analyze the optimal size for the location area of a distance-based registration (DBR) scheme by varying the number of location areas on a cell-by-cell basis, not on a ring-by-ring basis. Using our proposed cell-by-cell distance-based registration scheme with a random walk mobility model, a variety of circumstances were analyzed to obtain the optimal number of cells for location area for minimizing the total signaling cost on radio channels. Analysis results showed that the optimal number of cells for location area was between 4 and 7 in most cases. Our cell-by-cell distance-based location registration scheme had less signaling cost than an optimal ring-by-ring distance-based location registration scheme with an optimal distance threshold of 2 (the optimal number of cells for location area was 7). Therefore, when DBR is adopted, it must be implemented with an LA increasing on a cell-by-cell basis to achieve optimal performance.
Two-Step Biometrics Using Electromyogram Signal Based on Convolutional Neural Network-Long Short-Term Memory Networks
Authors: Kim Jin-Su , Kim Min-Gu , Pan Sung-Bum
Published: July 2021

Electromyogram (EMG) signals cannot be forged and have the advantage of being able to change the registered data as they are characterized by the waveform, which varies depending on the gesture. In this paper, a two-step biometrics method was proposed using EMG signals based on a convolutional neural network–long short-term memory (CNN-LSTM) network. After preprocessing of the EMG signals, the time domain features and LSTM network were used to examine whether the gesture matched, and single biometrics was performed if the gesture matched. In single biometrics, EMG signals were converted into a two-dimensional spectrogram, and training and classification were performed through the CNN-LSTM network. Data fusion of the gesture recognition and single biometrics was performed in the form of an AND. The experiment used Ninapro EMG signal data as the proposed two-step biometrics method, and the results showed 83.91% gesture recognition performance and 99.17% single biometrics performance. In addition, the false acceptance rate (FAR) was observed to have been reduced by 64.7% through data fusion.
On the Mechanical and Electrical Properties of the Composite Structure of PVC Membrane and Thin-Film Battery under Biaxial Tension
Authors: Ying Jingwei , Huang Junzhou , Qin Shengkun , Huang Yijie
Published: July 2021

This paper aims to study the mechanical and electrical properties of the composite structure of PVC film and film cell under biaxial tension. The saddle PVC membrane structure with thin-film battery was obtained by biaxial tensile tests carried out on the composite structure along the fiber direction and at an angle of 45 degrees to the fiber, respectively. The deformation of the film cell and PVC membrane materials was tested using digital image technology, and the voltage of the film cell was tested using a multimeter. The results showed that the tensile strain occurred in both membrane batteries and PVC membrane at different loading levels, and the former was always less than the latter. At a tensile load with the ultimate load ratio of 60%, it was only at the film cell’s outer edge that the stripping occurred. Under the illumination of a stable light source, the film cell voltage decreased gradually with the increasing tensile load. No more than 10% of the cell voltage drop occurred when the membrane material, the principal tensile strain of the cell, and the cell’s expansion area ratio were less than 3.1%, 2.8%, and 1.03, respectively. The experimental results show that the film cell can be applied to the saddle membrane structure by controlling the appropriate load.
A Multi-Loop Vehicle-Counting Method under Gray Mode and RGB Mode
Authors: Chen Yue , Lu Jian
Published: July 2021

With the rapid development of road traffic, real-time vehicle counting is very important in the construction of intelligent transportation systems (ITSs). Compared with traditional technologies, the video-based method for vehicle counting shows great importance and huge advantages in its low cost, high efficiency, and flexibility. However, many methods find difficulty in balancing the accuracy and complexity of the algorithm. For example, compared with traditional and simple methods, deep learning methods may achieve higher precision, but they also greatly increase the complexity of the algorithm. In addition to that, most of the methods only work under one mode of color, which is a waste of available information. Considering the above, a multi-loop vehicle-counting method under gray mode and RGB mode was proposed in this paper. Under gray and RGB modes, the moving vehicle can be detected more completely; with the help of multiple loops, vehicle counting could better deal with different influencing factors, such as driving behavior, traffic environment, shooting angle, etc. The experimental results show that the proposed method is able to count vehicles with more than 98.5% accuracy while dealing with different road scenes.
Effects of Cellulose Nanocrystal and Inorganic Nanofillers on the Morphological and Mechanical Properties of Digital Light Processing (DLP) 3D-Printed Photopolymer Composites
Authors: Bae Sang-U , Kim Birm-June
Published: July 2021

Photopolymer composites filled with cellulose nanocrystal (CNC) and/or inorganic nanofillers were fabricated by using digital light processing (DLP) 3D printing. To investigate the effects of different CNC lyophilization concentrations and behaviors of CNC particles in the photopolymer composites, morphological and mechanical properties were analyzed. CNC loading levels affected the morphological and mechanical properties of the filled composites. Better CNC dispersion was seen at a lower lyophilization concentration, and the highest mechanical strength was observed in the 0.25 wt% CNC-filled composite. Furthermore, nano-precipitated calcium carbonate (nano-PCC) and nanoclay were added to photocurable resins, and then the effect of inorganic nanofillers on the morphological and mechanical properties of the composites were evaluated. By analyzing the morphological properties, the stress transfer mechanism of nano-PCC and nanoclay in the photopolymer composites was identified and related models were presented. These supported the improved mechanical strength of the composites filled with CNC, nano-PCC, and nanoclay. This study suggested a new approach using wood-derived cellulose nanomaterials and inorganic nanofillers as effective fillers for DLP 3D printing.
Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo
Authors: Yoo Yeon-Jee , Kang Min-Ji , Perinpanayagam Hiran , Park Joo-Cheol , Baek Seung-Ho , Kum Kee-Yeon
Published: July 2021

This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (n = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (p < 0.05), without interactions with EDTA (p > 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.