bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Electrokinetic Remediation for the Removal of Heavy Metals from Contaminated Soils
Authors: Cameselle Claudio , Gouveia Susana , Cabo Adrian
Published: February 2021

The electrokinetic remediation of an agricultural soil contaminated with heavy metals was studied using organic acids as facilitating agents. The unenhanced electrokinetic treatment using deionized water as processing fluid did not show any significant mobilization and removal of heavy metals due to the low solubilization of metals and precipitation at high pH conditions close to the cathode. EDTA and citric acid 0.1 M were used as facilitating agents to favor the dissolution and transportation of metals. The organic acids were added to the catholyte and penetrated into the soil specimen by electromigration. EDTA formed negatively charged complexes. Citric acid formed neutral metal complexes in the soil pH conditions (pH = 2–4). Citric acid was much more effective in the dissolution and transportation out of the soil specimen of complexed metals. In order to enhance the removal of metals, the concentration of citric acid was increased up to 0.5 M, resulting in the removal of 78.7% of Cd, 78.6% of Co, 72.5% of Cu, 73.3% of Zn, 11.8% of Cr and 9.8% of Pb.
Melt Electrospinning of Polymers: Blends, Nanocomposites, Additives and Applications
Authors: Bachs-Herrera Anna , Yousefzade Omid , del Valle Luis J. , Puiggali Jordi
Published: February 2021

Melt electrospinning has been developed in the last decade as an eco-friendly and solvent-free process to fill the gap between the advantages of solution electrospinning and the need of a cost-effective technique for industrial applications. Although the benefits of using melt electrospinning compared to solution electrospinning are impressive, there are still challenges that should be solved. These mainly concern to the improvement of polymer melt processability with reduction of polymer degradation and enhancement of fiber stability; and the achievement of a good control over the fiber size and especially for the production of large scale ultrafine fibers. This review is focused in the last research works discussing the different melt processing techniques, the most significant melt processing parameters, the incorporation of different additives (e.g., viscosity and conductivity modifiers), the development of polymer blends and nanocomposites, the new potential applications and the use of drug-loaded melt electrospun scaffolds for biomedical applications.
Responses of Medicinal and Aromatic Plants to Engineered Nanoparticles
Authors: Kralova Katarina , Jampilek Josef
Published: February 2021

Medicinal and aromatic plants have been used by mankind since ancient times. This is primarily due to their healing effects associated with their specific secondary metabolites (some of which are also used as drugs in modern medicine), or their structures, served as a basis for the development of new effective synthetic drugs. One way to increase the production of these secondary metabolites is to use nanoparticles that act as elicitors. However, depending on the specific particle size, composition, concentration, and route of application, nanoparticles may have several other benefits on medicinal and aromatic plants (e.g., increased plant growth, improved photosynthesis, and overall performance). On the other hand, particularly at applications of high concentrations, they are able to damage plants mechanically, adversely affect morphological and biochemical characteristics of plants, and show cytotoxic and genotoxic effects. This paper provides a comprehensive overview of the beneficial and adverse effects of metal-, metalloid-, and carbon-based nanoparticles on the germination, growth, and biochemical characteristics of a wide range of medicinal and aromatic plants, including the corresponding mechanisms of action. The positive impact of nanopriming and application of nanosized fertilizers on medicinal and aromatic plants is emphasized. Special attention is paid to the effects of various nanoparticles on the production of valuable secondary metabolites in these plants cultivated in hydroponic systems, soil, hairy root, or in vitro cultures. The beneficial impact of nanoparticles on the alleviation of abiotic stresses in medicinal and aromatic plants is also discussed.
Numerical and Experimental Investigations on Vocal Fold Approximation in Healthy and Simulated Unilateral Vocal Fold Paralysis
Authors: Li Zheng , Wilson Azure , Sayce Lea , Avhad Amit , Rousseau Bernard , Luo Haoxiang
Published: February 2021

We have developed a novel surgical/computational model for the investigation of unilat-eral vocal fold paralysis (UVFP) which will be used to inform future in silico approaches to improve surgical outcomes in type I thyroplasty. Healthy phonation (HP) was achieved using cricothyroid suture approximation on both sides of the larynx to generate symmetrical vocal fold closure. Following high-speed videoendoscopy (HSV) capture, sutures on the right side of the larynx were removed, partially releasing tension unilaterally and generating asymmetric vocal fold closure characteristic of UVFP (sUVFP condition). HSV revealed symmetric vibration in HP, while in sUVFP the sutured side demonstrated a higher frequency (10–11%). For the computational model, ex vivo magnetic resonance imaging (MRI) scans were captured at three configurations: non-approximated (NA), HP, and sUVFP. A finite-element method (FEM) model was built, in which cartilage displacements from the MRI images were used to prescribe the adduction, and the vocal fold deformation was simulated before the eigenmode calculation. The results showed that the frequency comparison between the two sides was consistent with observations from HSV. This alignment between the surgical and computational models supports the future application of these methods for the investigation of treatment for UVFP.
An FPGA Based Energy Efficient DS-SLAM Accelerator for Mobile Robots in Dynamic Environment
Authors: Wu Yakun , Luo Li , Yin Shujuan , Yu Mengqi , Qiao Fei , Huang Hongzhi , Shi Xuesong , Wei Qi , Liu Xinjun
Published: February 2021

The Simultaneous Localization and Mapping (SLAM) algorithm is a hotspot in robot application research with the ability to help mobile robots solve the most fundamental problems of “localization” and “mapping”. The visual semantic SLAM algorithm fused with semantic information enables robots to understand the surrounding environment better, thus dealing with complexity and variability of real application scenarios. DS-SLAM (Semantic SLAM towards Dynamic Environment), one of the representative works in visual semantic SLAM, enhances the robustness in the dynamic scene through semantic information. However, the introduction of deep learning increases the complexity of the system, which makes it a considerable challenge to achieve the real-time semantic SLAM system on the low-power embedded platform. In this paper, we realized the high energy-efficiency DS-SLAM algorithm on the Field Programmable Gate Array (FPGA) based heterogeneous platform through the optimization co-design of software and hardware with the help of OpenCL (Open Computing Language) development flow. Compared with Intel i7 CPU on the TUM dataset, our accelerator achieves up to 13× frame rate improvement, and up to 18× energy efficiency improvement, without significant loss in accuracy.
Crosstalk between Different DNA Repair Pathways Contributes to Neurodegenerative Diseases
Authors: Gupta Swapnil , You Panpan , SenGupta Tanima , Nilsen Hilde , Sharma Kulbhushan
Published: February 2021

Genomic integrity is maintained by DNA repair and the DNA damage response (DDR). Defects in certain DNA repair genes give rise to many rare progressive neurodegenerative diseases (NDDs), such as ocular motor ataxia, Huntington disease (HD), and spinocerebellar ataxias (SCA). Dysregulation or dysfunction of DDR is also proposed to contribute to more common NDDs, such as Parkinson’s disease (PD), Alzheimer’s disease (AD), and Amyotrophic Lateral Sclerosis (ALS). Here, we present mechanisms that link DDR with neurodegeneration in rare NDDs caused by defects in the DDR and discuss the relevance for more common age-related neurodegenerative diseases. Moreover, we highlight recent insight into the crosstalk between the DDR and other cellular processes known to be disturbed during NDDs. We compare the strengths and limitations of established model systems to model human NDDs, ranging from C. elegans and mouse models towards advanced stem cell-based 3D models.
New Insights into the Antimicrobial Action of Cinnamaldehyde towards Escherichia coli and Its Effects on Intestinal Colonization of Mice
Authors: Pereira Wellison A. , Pereira Carlos Drielson S. , Assunção Raíssa G. , da Silva Iandeyara Savanna C. , Rego Fabrícia S. , Alves Leylane S. R. , Santos Juliana S. , Nogueira Francisco Jonathas R. , Zagmignan Adrielle , Thomsen Thomas T. , Løbner-Olesen Anders , Krogfelt Karen A. , da Silva Luís Cláudio N. , Abreu Afonso G.
Published: February 2021

Escherichia coli is responsible for cases of diarrhea around the world, and some studies have shown the benefits of cinnamaldehyde in the treatment of bacterial disease. Therefore, the objective of this study was to evaluate the effects of cinnamaldehyde in mice colonized by pathogenic E. coli, as well as to provide more insights into its antimicrobial action mechanism. After determination of minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations, the interference of cinnamaldehyde in macromolecular pathways (synthesis of DNA, RNA, protein, and cell wall) was measured by incorporation of radioisotopes. The anti-adhesive properties of cinnamaldehyde towards E. coli 042 were evaluated using human epithelial type 2 (HEp-2) cells. Intestinal colonization was tested on mice, and the effect of cinnamaldehyde on Tenebrio molitor larvae. Cinnamaldehyde showed MIC and MBC values of 780 μg/mL and 1560 μg/mL, respectively; reduced the adhesion of E. coli 042 on HEp-2 cells; and affected all the synthetic pathways evaluated, suggesting that compost impairs the membrane/cell wall structure leading bacteria to total collapse. No effect on the expression of genes related to the SOS pathway (sulA and dinB1) was observed. The compound did not interfere with cell viability and was not toxic against T. molitor larvae. In addition, cinnamaldehyde-treated mice exhibited lower levels of colonization by E. coli 042 than the untreated group. Therefore, the results show that cinnamaldehyde is effective in treating the pathogenic E. coli strain 042 and confirm it as a promising lead molecule for the development of antimicrobial agents.
Current Views on the Roles of O-Glycosylation in Controlling Notch-Ligand Interactions
Authors: Saiki Wataru , Ma Chenyu , Okajima Tetsuya , Takeuchi Hideyuki
Published: February 2021

The 100th anniversary of Notch discovery in Drosophila has recently passed. The Notch is evolutionarily conserved from Drosophila to humans. The discovery of human-specific Notch genes has led to a better understanding of Notch signaling in development and diseases and will continue to stimulate further research in the future. Notch receptors are responsible for cell-to-cell signaling. They are activated by cell-surface ligands located on adjacent cells. Notch activation plays an important role in determining the fate of cells, and dysregulation of Notch signaling results in numerous human diseases. Notch receptors are primarily activated by ligand binding. Many studies in various fields including genetics, developmental biology, biochemistry, and structural biology conducted over the past two decades have revealed that the activation of the Notch receptor is regulated by unique glycan modifications. Such modifications include O-fucose, O-glucose, and O-N-acetylglucosamine (GlcNAc) on epidermal growth factor-like (EGF) repeats located consecutively in the extracellular domain of Notch receptors. Being fine-tuned by glycans is an important property of Notch receptors. In this review article, we summarize the latest findings on the regulation of Notch activation by glycosylation and discuss future challenges.
Investigating the Effects of Seizures on Procedural Memory Performance in Patients with Epilepsy
Authors: van Schalkwijk Frank J. , Gruber Walter R. , Miller Laurie A. , Trinka Eugen , Höller Yvonne
Published: February 2021

Memory complaints are frequently reported by patients with epilepsy and are associated with seizure occurrence. Yet, the direct effects of seizures on memory retention are difficult to assess given their unpredictability. Furthermore, previous investigations have predominantly assessed declarative memory. This study evaluated within-subject effects of seizure occurrence on retention and consolidation of a procedural motor sequence learning task in patients with epilepsy undergoing continuous monitoring for five consecutive days. Of the total sample of patients considered for analyses (N = 53, Mage = 32.92 ± 13.80 y, range = 18–66 y; 43% male), 15 patients experienced seizures and were used for within-patient analyses. Within-patient contrasts showed general improvements over seizure-free (day + night) and seizure-affected retention periods. Yet, exploratory within-subject contrasts for patients diagnosed with temporal lobe epilepsy (n = 10) showed that only seizure-free retention periods resulted in significant improvements, as no performance changes were observed following seizure-affected retention. These results indicate general performance improvements and offline consolidation of procedural memory during the day and night. Furthermore, these results suggest the relevance of healthy temporal lobe functioning for successful consolidation of procedural information, as well as the importance of seizure control for effective retention and consolidation of procedural memory.
Performance Characteristics of the Ultrasound Strategy during Incidence Screening in the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS)
Authors: Kalsi Jatinderpal , Gentry-Maharaj Aleksandra , Ryan Andy , Singh Naveena , Burnell Matthew , Massingham Susan , Apostolidou Sophia , Sharma Aarti , Williamson Karin , Seif Mourad , Mould Tim , Woolas Robert , Dobbs Stephen , Leeson Simon , Fallowfield Lesley , Skates Steven J. , Parmar Mahesh , Campbell Stuart , Jacobs Ian , McGuire Alistair , Menon Usha
Published: February 2021

Randomised controlled trials of ovarian cancer (OC) screening have not yet demonstrated an impact on disease mortality. Meanwhile, the screening data from clinical trials represents a rich resource to understand the performance of modalities used. We report here on incidence screening in the ultrasound arm of UKCTOCS. 44,799 of the 50,639 women who were randomised to annual screening with transvaginal ultrasound attended annual incidence screening between 28 April 2002 and 31 December 2011. Transvaginal ultrasound was used both as the first and the second line test. Participants were followed up through electronic health record linkage and postal questionnaires. Out of 280,534 annual incidence screens, 960 women underwent screen-positive surgery. 113 had ovarian/tubal cancer (80 invasive epithelial). Of the screen-detected invasive epithelial cancers, 37.5% (95% CI: 26.9–49.0) were Stage I/II. An additional 52 (50 invasive epithelial) were diagnosed within one year of their last screen. Of the 50 interval epithelial cancers, 6.0% (95% CI: 1.3–16.5) were Stage I/II. For detection of all ovarian/tubal cancers diagnosed within one year of screen, the sensitivity, specificity, and positive predictive values were 68.5% (95% CI: 60.8–75.5), 99.7% (95% CI: 99.7–99.7), and 11.8% (95% CI: 9.8–14) respectively. When the analysis was restricted to invasive epithelial cancers, sensitivity, specificity and positive predictive values were 61.5% (95% CI: 52.6–69.9); 99.7% (95% CI: 99.7–99.7) and 8.3% (95% CI: 6.7–10.3), with 12 surgeries per screen positive. The low sensitivity coupled with the advanced stage of interval cancers suggests that ultrasound scanning as the first line test might not be suitable for population screening for ovarian cancer. Trial registration: ISRCTN22488978. Registered on 6 April 2000.
Expression of Genomic Instability-Related Molecules: Cyclin F, RRM2 and SPDL1 and Their Prognostic Significance in Pancreatic Adenocarcinoma
Authors: Klimaszewska-Wiśniewska Anna , Buchholz Karolina , Neska-Długosz Izabela , Durślewicz Justyna , Grzanka Dariusz , Zabrzyński Jan , Sopońska Paulina , Grzanka Alina , Gagat Maciej
Published: February 2021

In the present study, we aimed to assess the selected components of cell cycle machinery, checkpoint, DNA repair, and synthesis, namely RRM2, cyclin F, and SPDL1 in pancreatic adenocarcinomas (PAC) by in-house immunohistochemistry (IHC) and bioinformatic analysis of public datasets, in terms of expression, correlation with clinicopathological parameters, and patient survival. Sixty eight patients with pancreatic ductal adenocarcinoma (PDAC) were included in our cohort study, and IHC was performed on tissue macroarrays. RNA-Seq-based transcriptome data for 177 PACs were retrieved from the Cancer Genome Atlas (TCGA). We found cyclin F, RRM2, and SPDL1 to be overexpressed at both protein and mRNA levels in tumor tissues compared to respective controls. Based on TCGA dataset, we have demonstrated that CCNF, RRM2, and SPDL1 are potent independent prognostic markers for poor overall survival, both by themselves and even more in combination with each other. Furthermore, high CCNF mRNA expression was associated with features of cancer progression. By contrast, overexpression of cyclin F or SPDL1 proteins denoted a good prognosis in PDAC patients; however, in the case of the former protein, the results did not reach statistical significance. Specifically, high levels of SPDL1 protein emerged as the most powerful independent prognostic factor associated with a better outcome. If validated, the CCNF/RRM2/SPDL1 three-gene panel developed in this study, as well as SPDL1 protein, may provide significant clinical implications for the prognosis prediction of PAC patients.
RNA-Seq Analysis Reveals CCR5 as a Key Target for CRISPR Gene Editing to Regulate In Vivo NK Cell Trafficking
Authors: Levy Emily R. , Clara Joseph A. , Reger Robert N. , Allan David S. J. , Childs Richard W.
Published: February 2021

A growing number of natural killer (NK) cell-based immunotherapy trials utilize ex vivo expansion to grow and activate allogenic and autologous NK cells prior to administration to patients with malignancies. Recent data in both murine and macaque models have shown that adoptively infused ex vivo expanded NK cells have extensive trafficking into liver tissue, with relatively low levels of homing to other sites where tumors often reside, such as the bone marrow or lymph nodes. Here, we evaluated gene and surface expression of molecules involved in cellular chemotaxis in freshly isolated human NK cells compared with NK cells expanded ex vivo using two different feeder cells lines: Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) or K562 cells with membrane-bound (mb) 4-1BB ligand and interleukin (IL)-21. Expanded NK cells had altered expression in a number of genes that encode chemotactic ligands and chemotactic receptors that impact chemoattraction and chemotaxis. Most notably, we observed drastic downregulation of C-X-C chemokine receptor type 4 (CXCR4) and upregulation of C-C chemokine receptor type 5 (CCR5) transcription and phenotypic expression. clustered regularly interspaced short palindromic repeats (CRISPR) gene editing of CCR5 in expanded NK cells reduced cell trafficking into liver tissue and increased NK cell presence in the circulation following infusion into immunodeficient mice. The findings reported here show that ex vivo expansion alters multiple factors that govern NK cell homing and define a novel approach using CRISPR gene editing that reduces sequestration of NK cells by the liver.
Worm-Based Microfluidic Biosensor for Real-Time Assessment of the Metastatic Status
Authors: Zhang Jing , Chua Song Lin , Khoo Bee Luan
Published: February 2021

Background: Metastasis is a complex process that affects patient treatment and survival. To routinely monitor cancer plasticity and guide treatment strategies, it is highly desired to provide information about metastatic status in real-time. Here, we proposed a worm-based (WB) microfluidic biosensor to rapidly monitor biochemical cues related to metastasis in a well-defined environment. Compared to conventional biomarker-based methods, the WB biosensor allowed high throughput screening under low cost, requiring only visual quantification of outputs; Methods: Caenorhabditis elegans were placed in the WB biosensor and exposed to samples conditioned with cancer cell clusters. The chemotactic preference of these worms was observed under discontinuous imaging to minimize the impact on physiological activity; Results: A chemotaxis index (CI) was defined to standardize the quantitative assessment from the WB biosensor, where moderate (3.24–6.5) and high (>6.5) CI levels reflected increased metastasis risk and presence of metastasis, respectively. We demonstrated that the secreted metabolite glutamate was a chemorepellent, and larger clusters associated with increased metastatic potential also enhanced CI levels; Conclusions: Overall, this study provided a proof of concept for the WB biosensors in assessing metastasis status, with the potential to evaluate patient-derived cancer clusters for routine management.
Gastric Cancer Risk Prediction Using an Epidemiological Risk Assessment Model and Polygenic Risk Score
Authors: Park Boyoung , Yang Sarah , Lee Jeonghee , Choi Il Ju , Kim Young-Il , Kim Jeongseon
Published: February 2021

We investigated the performance of a gastric cancer (GC) risk assessment model in combination with single-nucleotide polymorphisms (SNPs) as a polygenic risk score (PRS) in consideration of Helicobacter pylori (H. pylori) infection status. Six SNPs identified from genome-wide association studies and a marginal association with GC in the study population were included in the PRS. Discrimination of the GC risk assessment model, PRS, and the combination of the two (PRS-GCS) were examined regarding incremental risk and the area under the receiver operating characteristic curve (AUC), with grouping according to H. pylori infection status. The GC risk assessment model score showed an association with GC, irrespective of H. pylori infection. Conversely, the PRS exhibited an association only for those with H. pylori infection. The PRS did not discriminate GC in those without H. pylori infection, whereas the GC risk assessment model showed a modest discrimination. Among individuals with H. pylori infection, discrimination by the GC risk assessment model and the PRS were comparable, with the PRS-GCS combination resulting in an increase in the AUC of 3%. In addition, the PRS-GCS classified more patients and fewer controls at the highest score quintile in those with H. pylori infection. Overall, the PRS-GCS improved the identification of a GC-susceptible population of people with H. pylori infection. In those without H. pylori infection, the GC risk assessment model was better at identifying the high-risk group.
Primary Effusion Lymphoma: A Clinicopathological Study of 70 Cases
Authors: Hu Zhihong , Pan Zenggang , Chen Weina , Shi Yang , Wang Wei , Yuan Ji , Wang Endi , Zhang Shanxiang , Kurt Habibe , Mai Brenda , Zhang Xiaohui , Liu Hui , Rios Adan A. , Ma Hilary Y. , Nguyen Nghia D. , Medeiros L. Jeffrey , Hu Shimin
Published: February 2021

Primary effusion lymphoma (PEL) is a rare type of large B-cell lymphoma associated with human herpesvirus 8 (HHV8) infection. Patients with PEL usually present with an effusion, but occasionally with an extracavitary mass. In this study, we reported a cohort of 70 patients with PEL: 67 men and 3 women with a median age of 46 years (range 26–91). Of these, 56 (80%) patients had human immunodeficiency virus (HIV) infection, eight were HIV-negative, and six had unknown HIV status. Nineteen (27%) patients had Kaposi sarcoma. Thirty-five (50%) patients presented with effusion only, 27 (39%) had an extracavitary mass or masses only, and eight (11%) had both effusion and extracavitary disease. The lymphoma cells showed plasmablastic, immunoblastic, or anaplastic morphology. All 70 (100%) cases were positive for HHV8. Compared with effusion-only PEL, patients with extracavitary-only PEL were younger (median age, 42 vs. 52 years, p = 0.001), more likely to be HIV-positive (88.9% vs. 68.6%, p = 0.06) and EBV-positive (76.9% vs. 51.9%, p = 0.06), and less often positive for CD45 (69.2% vs. 96.2%, p = 0.01), EMA (26.7% vs. 100%, p = 0.0005), and CD30 (60% vs. 81.5%, p = 0.09). Of 52 (50%) patients with clinical follow-up, 26 died after a median follow-up time of 40.0 months (range 0–96), and the median overall survival was 42.5 months. The median OS for patients with effusion-only and with extracavitary-only PEL were 30.0 and 37.9 months, respectively (p = 0.34), and patients with extracavitary-only PEL had a lower mortality rate at the time of last follow-up (35% vs. 61.5%, p = 0.07). The median OS for HIV-positive and HIV-negative patients were 42.5 and 6.8 months, respectively (p = 0.57), and they had a similar mortality rate of 50% at last follow-up. In conclusion, patients presenting with effusion-only versus extracavitary-only disease are associated with different clinicopathologic features. PEL is an aggressive lymphoma with a poor prognosis, regardless of extracavitary presentation or HIV status.
Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGFβ/CTGF Axis
Authors: Hinz Nico , Baranowsky Anke , Horn Michael , Kriegs Malte , Sibbertsen Freya , Smit Daniel J. , Clezardin Philippe , Lange Tobias , Schinke Thorsten , Jücker Manfred
Published: February 2021

Bone metastases frequently occur in breast cancer patients and lack appropriate treatment options. Hence, understanding the molecular mechanisms involved in the multistep process of breast cancer bone metastasis and tumor-induced osteolysis is of paramount interest. The serine/threonine kinase AKT plays a crucial role in breast cancer bone metastasis but the effect of individual AKT isoforms remains unclear. Therefore, AKT isoform-specific knockdowns were generated on the bone-seeking MDA-MB-231 BO subline and the effect on proliferation, migration, invasion, and chemotaxis was analyzed by live-cell imaging. Kinome profiling and Western blot analysis of the TGFβ/CTGF axis were conducted and metastasis was evaluated by intracardiac inoculation of tumor cells into NOD scid gamma (NSG) mice. MDA-MB-231 BO cells exhibited an elevated AKT3 kinase activity in vitro and responded to combined treatment with AKT- and mTOR-inhibitors. Knockdown of AKT3 significantly increased migration, invasion, and chemotaxis in vitro and metastasis to bone but did not significantly enhance osteolysis. Furthermore, knockdown of AKT3 increased the activity and phosphorylation of pro-metastatic HER2 and DDR1/2 but lowered protein levels of CTGF after TGFβ-stimulation, an axis involved in tumor-induced osteolysis. We demonstrated that AKT3 plays a crucial role in bone-seeking breast cancer cells by promoting metastatic potential without facilitating tumor-induced osteolysis.
Suppression of the HBP Function Increases Pancreatic Cancer Cell Sensitivity to a Pan-RAS Inhibitor
Authors: Ricciardiello Francesca , Bergamaschi Laura , De Vitto Humberto , Gang Yang , Zhang Taiping , Palorini Roberta , Chiaradonna Ferdinando
Published: February 2021

Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer-related death and the search for a resolutive therapy is still a challenge. Since KRAS is commonly mutated in PDAC and is one of the main drivers of PDAC progression, its inhibition should be a key strategy for treatment, especially considering the recent development of specific KRAS inhibitors. Nevertheless, the effects of KRAS inhibition can be increased through the co-inhibition of other nodes important for cancer development. One of them could be the hexosamine biosynthetic pathway (HBP), whose enhancement is considered fundamental for PDAC. Here, we demonstrate that PDAC cells expressing oncogenic KRAS, owing to an increase in the HBP flux, become strongly reliant on HBP for both proliferation and survival. In particular, upon treatment with two different compounds, 2-deoxyglucose and FR054, inhibiting both HBP and protein N-glycosylation, these cells undergo apoptosis significantly more than PDAC cells expressing wild-type KRAS. Importantly, we also show that the combined treatment between FR054 and the pan-RAS inhibitor BI-2852 has an additive negative effect on cell proliferation and survival by means of the suppression of both Akt activity and cyclin D1 expression. Thus, co-inhibition of HBP and oncogenic RAS may represent a novel therapy for PDAC patients.
Statins as a Therapeutic Approach for the Treatment of Pseudoxanthoma Elasticum Patients: Evaluation of the Spectrum Efficacy of Atorvastatin In Vitro
Authors: Tiemann Janina , Lindenkamp Christopher , Plümers Ricarda , Faust Isabel , Knabbe Cornelius , Hendig Doris
Published: February 2021

Pseudoxanthoma elasticum (PXE) is an autosomal recessive disorder caused by mutations in the ATP-binding cassette sub-family C member 6 gene. Our previous studies revealed that PXE might be associated with premature aging. Treatment with statins showed positive effects not only for PXE but also for other diseases associated with premature aging like Hutchinson–Gilford progeria syndrome. Nevertheless, the molecular mechanisms in the case of PXE remain unclear. Thus, this study was performed to evaluate the efficiency of atorvastatin by analyzing key characteristics of the PXE phenotype in primary human dermal fibroblasts of PXE patients. Our data indicate that an atorvastatin treatment has a positive effect, especially on factors associated with cholesterol biosynthesis and prenylation processes, whereas the effect on age- and calcification-related factors was less pronounced.
Development of Adhesive, Bioactive and Antibacterial Titania Sol-Gel Coating on Titanium Substrate by Dip-Coating Technique
Authors: Horkavcová Diana , Doubet Quentin , Lecomte-Nana Gisèle Laure , Jablonská Eva , Helebrant Aleš
Published: February 2021

The sol-gel method provides a wide variety of applications in the medical field. One of these applications is the formation of coatings on the metal implants. The coatings containing specific additive can enhance or improve the existing surface properties of the substrate. In this work, titania sol-gel coatings were doped with two forms of silver (AgNO3, Ag3PO4) and synthetic hydroxyapatite and applied on the titanium samples by dip-coating technique. After drying and slow firing, all coatings were characterized with scanning electron microscopy. Thin coatings were successfully prepared with excellent adhesion to the substrate (measured by ASTM D 3359-2), despite cracks. Coatings containing silver and hydroxyapatite demonstrated a 100% antibacterial effect against Escherichia coli after 24 h. The bioactivity of the coatings containing hydroxyapatite tested in modified simulated body fluid under static-dynamic conditions was confirmed by bone-like hydroxyapatite precipitation. To better understand the interaction of the coatings with simulated body fluid (SBF), changes of Ca2+ and (PO4)3− ions concentrations and pH values were studied.
Measurement of the Elastic Modulus and Residual Stress of Thermal Barrier Coatings Using a Digital Image Correlation Technique
Authors: Zhu Qi , Zeng Yuchun , Yang Dong , Zhu Jianguo , Zhuo Lijun , Li Jian , Xie Weihua
Published: February 2021

This paper presents an experimental study on simultaneously measuring the elastic modulus and residual stress of a thermal barrier coating (TBC) after different isothermal heat treatments. The elastic modulus and residual stress of TBCs were theoretically analyzed based on composite beam bending theory. Thereafter, an experimental setup was established combining the 3D digital image correlation method with the bending test to obtain the curvature changes in the TBC sample. Finally, the elastic modulus and residual stress of the ceramic layer with different isothermal heat treatments were obtained. The results show that the elastic modulus of the ceramic layer measured under compression is greater than that under tension, and the elastic modulus of the ceramic layer increases first and then tends to be stable as the heat treatment time increases. In addition, the residual stress of the TBCs ceramic layer quickly changes from compressive stress to tensile stress with heat treatment, and the tensile stress increases with the increase in thermal exposure time. Furthermore, the reasons for the change tendency were analyzed according to the variation in porosity and microstructures by processing the scanning electron microscope (SEM) figures. The results demonstrate that simultaneously determining the elastic modulus and residual stress of TBC based on combining the 3D digital image correlation method with the bending test is effective and reliable.
Spherical Droplet Deposition—Mechanistic Model
Authors: Michalski Jacek A. , Jakiela Slawomir
Published: February 2021

In the currently existing physical models of wetting a solid substrate by a liquid drop, the contact angle is determined on the basis of the equilibrium of forces acting tangentially to the wetted surface at any point in the perimeter of the wetted area, ignoring the forces (or their components) acting perpendicular to this area. In the solution shown in the paper, the equilibrium state of forces acting on a droplet was determined based on the minimum mechanical energy that the droplet achieves in the state of equilibrium. This approach allows one to take into account in the model, in addition to the forces tangential to the wetted surface, also forces perpendicular to it (also the force of adhesion), moreover, these may be dispersed forces acting on the entire interface, not on a single point. The correctness of this approach is confirmed by the derived equations concerning the forces acting on the liquid both tangentially and perpendicularly to the wetted surface. The paper also identifies the areas of solutions in which the obtained equilibrium of forces is stable and areas of unstable equilibrium of forces. The solution is formulated both for isothermal and isochoric system. Based on the experimental data accessible in the literature, the condition that has to be met by the droplets (and their surroundings) during measurements performed under gravity conditions was formulated.
On the Systematics and Biodiversity of the Opheliidae and Scalibregmatidae
Authors: Parapar Julio , Martínez Alejandro , Moreira Juan
Published: February 2021

In this paper we review the systematics, diversity, and ecology of two related annelid families: Opheliidae Malmgren, 1867 and Scalibregmatidae Malmgren, 1867. Opheliids are deposit-feeders and that are mainly found as burrowers in sandy sediments. Morphologically, opheliids are characterized by the smooth cuticle, as well as the presence of a conspicuous ventral groove, reduced parapodia, and a tubular-shaped structure often projecting from the posterior end. Scalibregmatids are also deposit-feeders, but compared to opheliids, they have a characteristic arenicoliform body, a T-shaped anterior end and a glandular, reticulated epidermis. For each family, we summarize the available information about the evolutionary relationships, taxonomic history, geographical distribution, ecological preferences and diversity of life strategies along with the techniques most commonly used for their study. By highlighting the main gaps in knowledge on each of these topics, this review ultimately aims at stimulating further research into members of these two families in the future.
Biogeography of Iberian Ants (Hymenoptera: Formicidae)
Authors: Tinaut Alberto , Ruano Francisca
Published: February 2021

Ants are highly diverse in the Iberian Peninsula (IP), both in species richness (299 cited species) and in number of endemic species (72). The Iberian ant fauna is one of the richest in the broader Mediterranean region, it is similar to the Balkan Peninsula but lower than Greece or Israel, when species richness is controlled by the surface area. In this first general study on the biogeography of Iberian ants, we propose seven chorological categories for grouping thems. Moreover, we also propose eight biogeographic refugium areas, based on the criteria of “refugia-within-refugium” in the IP. We analysed species richness, occurrence and endemism in all these refugium areas, which we found to be significantly different as far as ant similarity was concerned. Finally, we collected published evidence of biological traits, molecular phylogenies, fossil deposits and geological processes to be able to infer the most probable centre of origin and dispersal routes followed for the most noteworthy ants in the IP. As a result, we have divided the Iberian myrmecofauna into four biogeographical groups: relict, Asian-IP disjunct, Baetic-Rifan and Alpine. To sum up, our results support biogeography as being a significant factor for determining the current structure of ant communities, especially in the very complex and heterogenous IP. Moreover, the taxonomic diversity and distribution patterns we describe in this study highlight the utility of Iberian ants for understanding the complex evolutionary history and biogeography of the Iberian Peninsula.
Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings
Authors: Morris Ulrika , Aydin-Schmidt Berit
Published: February 2021

Loop-mediated isothermal amplification (LAMP) is a sensitive molecular tool suitable for use as a near point-of-care test for the diagnosis of malaria. Recent meta-analyses have detailed high sensitivity and specificity of malaria LAMP when compared to microscopy, rapid diagnostic tests, and polymerase chain reaction in both endemic and non-endemic settings. Despite this, the use of malaria LAMP has primarily been limited to research settings to date. In this review, we aim to assess to what extent commercially available malaria LAMP kits have been applied in different settings, and to identify possible obstacles that may have hindered their use from being adopted further. In order to address this, we conducted a literature search in PubMed.gov using the search terms (((LAMP) OR (Loop-mediated isothermal amplification)) AND ((Malaria) OR (Plasmodium))). Focusing primarily on studies employing one of the commercially available kits, we then selected three key areas of LAMP application for further review: the performance and application of LAMP in malaria endemic settings including low transmission areas; LAMP for malaria screening during pregnancy; and malaria LAMP in returning travelers in non-endemic settings.
The Role of the Pathologist in the Next-Generation Era of Tumor Molecular Characterization
Authors: Angerilli Valentina , Galuppini Francesca , Pagni Fabio , Fusco Nicola , Malapelle Umberto , Fassan Matteo
Published: February 2021

Current pathology practice is being shaped by the increasing complexity of modern medicine, in particular of precision oncology, and major technological advances. In the “next-generation technologies era”, the pathologist has become the person responsible for the integration and interpretation of morphologic and molecular information and for the delivery of critical answers to diagnostic, prognostic and predictive queries, acquiring a prominent position in the molecular tumor boards.
Robustness of Laser Speckles as Unique Traceable Markers of Metal Components
Authors: Sjödahl Mikael , Olsson Erik
Published: February 2021

The traceability of manufactured components is growing in importance with the greater use of digital service solutions offered and with an increased digitalization of manufacturing logistics. In this paper, we investigate the use of image-plane laser speckles as a tool to acquire a unique code from the surface of the component and the ability to use this pattern as a secure component-specific digital fingerprint. Intensity correlation is used as a numerical identifier. Metal sheets of different materials and steel pipes are considered. It is found that laser speckles are robust against surface alterations caused by surface compression and scratching and that the correct pattern reappears from a surface contaminated by oil after cleaning. In this investigation, the detectability is close to 100% for all surfaces considered, with zero false positives. The exception is a heavily oxidized surface wiped by a cotton cloth between recordings. It is further found that the main source for lost detectability is caused by misalignment between the registration and detection geometries where a positive match is lost by a change in angle in the order of 60 mrad. Therefore, as long as the registration and detection systems, respectively, use the same optical arrangement, laser speckles have the ability to serve as unique component identifiers without having to add extra markings or a dedicated sensor to the component.
Transmitter Diversity Gain Technique Aided Irregular Channel Coding for Mobile Video Transmission
Authors: Minallah Nasru , Ullah Khadem , Frnda Jaroslav , Cengiz Korhan , Awais Javed Muhammad
Published: February 2021

The reliable transmission of multimedia information that is coded through highly compression efficient encoders is a challenging task. This article presents the iterative convergence performance of IrRegular Convolutional Codes (IRCCs) with the aid of the multidimensional Sphere Packing (SP) modulation assisted Differential Space Time Spreading Codes (IRCC-SP-DSTS) scheme for the transmission of H.264/Advanced Video Coding (AVC) compressed video coded stream. In this article, three different regular and irregular error protection schemes are presented. In the presented Regular Error Protection (REP) scheme, all of the partitions of the video sequence are regular error protected with a rate of 3/4 IRCC. In Irregular Error Protection scheme-1 (IREP-1) the H.264/AVC partitions are prioritized as A, B & C, respectively. Whereas, in Irregular Error Protection scheme-2 (IREP-2), the H.264/AVC partitions are prioritized as B, A, and C, respectively. The performance of the iterative paradigm of an inner IRCC and outer Rate-1 Precoder is analyzed by the EXtrinsic Information Transfer (EXIT) Chart and the Quality of Experience (QoE) performance of the proposed mechanism is evaluated using the Bit Error Rate (BER) metric and Peak Signal to Noise Ratio (PSNR)-based objective quality metric. More specifically, it is concluded that the proposed IREP-2 scheme exhibits a gain of 1 dB Eb/N0 with reference to the IREP-1 and Eb/N0 gain of 0.6 dB with reference to the REP scheme over the PSNR degradation of 1 dB.
Home Energy Management for Community Microgrids Using Optimal Power Sharing Algorithm
Authors: Rashid Md Mamun Ur , Alotaibi Majed A. , Chowdhury Abdul Hasib , Rahman Muaz , Alam Md. Shafiul , Hossain Md. Alamgir , Abido Mohammad A.
Published: February 2021

From a residential point of view, home energy management (HEM) is an essential requirement in order to diminish peak demand and utility tariffs. The integration of renewable energy sources (RESs) together with battery energy storage systems (BESSs) and central battery storage system (CBSS) may promote energy and cost minimization. However, proper home appliance scheduling along with energy storage options is essential to significantly decrease the energy consumption profile and overall expenditure in real-time operation. This paper proposes a cost-effective HEM scheme in the microgrid framework to promote curtailing of energy usage and relevant utility tariff considering both energy storage and renewable sources integration. Usually, the household appliances have different runtime preferences and duration of operation based on user demand. This work considers a simulator designed in the C++ platform to address the domestic customer’s HEM issue based on usages priorities. The positive aspects of merging RESs, BESSs, and CBSSs with the proposed optimal power sharing algorithm (OPSA) are evaluated by considering three distinct case scenarios. Comprehensive analysis of each scenario considering the real-time scheduling of home appliances is conducted to substantiate the efficacy of the outlined energy and cost mitigation schemes. The results obtained demonstrate the effectiveness of the proposed algorithm to enable energy and cost savings up to 37.5% and 45% in comparison to the prevailing methodology.
Clarifications on the Behavior of Alternative Gases to SF6 in Divergent Electric Field Distributions under AC Voltage
Authors: Nechmi Houssem Eddine , Michelarakis Michail , (Manu) Haddad Abderrahmane , Wilson Gordon
Published: February 2021

Negative and positive partial discharge inception voltages and breakdown measurements are reported in a needle-plane electrode system as a function of pressure under AC voltage for natural gases (N2, CO2, and O2/CO2), pure NovecTM gases (C4F7N and C5F10O) and NovecTM in different natural gas admixtures. For compressed 4% C4F7N–96% CO2 and 6% C5F10O–12% O2–82% CO2 gas mixtures, the positive-streamer mode is identified as the breakdown mechanism. Breakdown and negative partial discharge inception voltages of 6% C5F10O–12% O2–82% CO2 are higher than those of 4% C4F7N–96% CO2. At 8.8 bar abs, the breakdown voltage of 6% C5F10O–12% O2–82% CO2 is equal to that of 12.77% O2–87.23% CO2 (buffer gas). Synergism in negative partial discharge inception voltage/electric field fits with the mean value and the sum of each partial pressure individually component for a 20% C4F7N–80% CO2 and 6% C5F10O–12% O2–82% CO2, respectively. In 9% C4F7N–91% CO2, the comparison of partial discharge inception electric fields is Emax (CO2) = Emax(C4F7N), and Emax (12.77% O2–87.23% CO2) = Emax(C5F10O) in 19% C5F10O–81%(12.77% O2–87.23% CO2). Polarity reversal occurs under AC voltage when the breakdown polarity changes from negative to positive cycle. Polarity reversal electric field EPR was quantified. Fitting results show that EPR (CO2) = EPR(9% C4F7N–91% CO2) and EPR(SF6) = EPR (22% C4F7N–78% CO2). EPR (4% C4F7N–96% CO2) = EPR (12.77% O2–87.23% CO2) and EPR (6% C5F10O–12% O2–82% CO2) < EPR (4% C4F7N–96% CO2) < EPR (CO2).
Performance Characteristics of a Dual-Stator, Spoke-Type Permanent Magnet Vernier Machine with Support Bar
Authors: Park Jang-Hyun , Lukman Grace Firsta , Kang Do-Hyun , Ahn Jin-Woo
Published: February 2021

Permanent magnet Vernier machine (PMVM) is a strong candidate for direct-drive applications in low-speed region because its power characteristic is higher than conventional permanent magnet machine (PMM). In this paper, the design of a dual-stator PMVM (DSPMVM) with spoke-type rotor is introduced. As a radial motor with dual-stator configuration, one on the outer and inner side, the rotor is equipped with support bars for practical and simple manufacturing, which is the aim of this study. The characteristics and performance of the proposed machine with and without the support bar are examined with finite element analysis (FEA). The DSPMVM and the support were manufactured and tested through experiments to verify proposed structure. Both simulation and experiment results show that there is little to no difference in performance when the support bar is equipped. Furthermore, the average deviation between simulation and experiment results is approximately 7% which is within the acceptable range.