bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Membrane Techniques for Removal and Recovery of Nutrients from Liquid Fraction of Anaerobic Digestate
Authors: Zielińska Magdalena , Bułkowska Katarzyna
Published: February 2025

This review focuses on the use of membrane techniques to recover nutrients from the liquid fraction of digestate (LFD) and emphasizes their role in promoting the principles of the circular economy. A range of membrane separation processes are examined, including microfiltration (MF), ultrafiltration (UF), nanofiltration (NF), reverse osmosis (RO), forward osmosis (FO), membrane distillation (MD) and new tools and techniques such as membrane contactors (MCs) with gas-permeable membranes (GPMs) and electrodialysis (ED). Key aspects that are analyzed include the nutrient concentration efficiency, integration with biological processes and strategies to mitigate challenges such as fouling, high energy requirements and scalability. In addition, innovative hybrid systems and pretreatment techniques are examined for their potential to improve the recovery rates and sustainability. The review also addresses the economic and technical barriers to the full-scale application of these technologies and identifies future research directions, such as improving the membrane materials and reducing the energy consumption. The comprehensive assessment of these processes highlights their contribution to sustainable nutrient management and bio-based fertilizer production.
Anisotropic Hardening of HC420 Steel Sheet: Experiments and Analytical Modeling
Authors: Alhalaybeh Thamer Sami , Akhtar Hammad , Chowdhury Ashiq Iqbal , Lou Yanshan
Published: February 2025

Choosing the appropriate yield function is essential to precisely predicting the anisotropic hardening behavior of steel metals considering general loading directions. This research investigates the anisotropic hardening behavior of HC420 steel sheet by combining experimental and analytical modeling. Experiments are conducted for uniaxial tensile tests according to the three different directions and bulging tests to obtain hardening data. The experimental findings show that the loading direction affects the anisotropic behavior of HC420 steel’s strength and plastic deformation. The Chen-coupled quadratic and non-quadratic (Chen-CQN) approach is used to ensure the convexity of the HC420 steel. By comparing the Chen-CQN approach with the Yld2000-2d and Stoughton-Yoon’2009 yield functions, the Chen-CQN approach shows superiority in predicting the hardening behavior of the HC420 sheet, exhibiting a more straightforward numerical implementation and enhanced accuracy in yield stress predictions under different loading directions. Results from experimental hardening tests reveal that the Chen-CQN function precisely and flexibly characterizes the yield surface of HC420 steel, with a constant variation of within 2% from its predictions.
The Effect of Final Cooling Temperature on Nano Cu Precipitation in a Cu-Bearing High-Strength Low-Alloy Steel
Authors: Cui Haitao , Liang Haicheng , Sun Xinglong , Li Yonghua , Gao Zhanjie , Liu Jinsong
Published: February 2025

Nano Cu precipitation plays a crucial role in significantly improving the performance of the Cu-bearing high-strength low-alloy steel. The final cooling temperature effects the transformation products of austenite during the continuous cooling process, as well as the nano precipitations of steel. This study investigated the microstructure and hardness at different final cooling temperatures (750, 700, 650, 600, 550, and 500 °C) using the MMS-300 thermal simulation experimental machine (Northeastern University, Shenyang, China) and Vickers hardness tester. The changes in microstructure and the phase transformation law of austenite were determined during continuous cooling and then analyzed. The precipitation reaction of nano Cu precipitation during continuous cooling was studied using transmission electron microscopy (TEM), revealing the precipitation state under different final cooling temperature conditions. The results showed that the precipitations led to an increase and then a decrease in the microhardness, and the microhardness reaches its peak at 550 °C. The precipitations changed from spherical to elliptical, and the size gradually increased when the final cooling temperature increased.
Research Progress on Development of PVP-Ag-Doped LaMnO3 Composites for Methyl Orange Degradation
Authors: Căta Adina , Ivanovici Madalina-Gabriela , Svera Paula , Ienașcu Ioana Maria Carmen , Sfirloaga Paula
Published: February 2025

Water pollution caused by emerging contaminants is increasing due to rising urbanization, industrialization, and agriculture production; therefore, new materials with high efficiency for wastewater decontamination are needed. A perovskite material based on 1% Ag-doped LaMnO3 synthesized through a sol–gel technique was combined with PVP in a 1:10 (w/w) ratio and subjected to different temperature and microwave conditions at various time intervals. The composite materials were obtained as thin films (S1, S2) or powders (S3) and were analyzed by modern techniques. The SEM analysis showed strongly agglomerated, asymmetric formations for the S1, S2 materials; as for the S3 composite, irregularly shaped grains of perovskite were deposited on the polymer surface. Small, round formations across the surface, mainly organized as clusters with conic/square-shaped particles and observed asperity on top, were highlighted by AFM images. The XRD spectra confirmed the presence of both the perovskite and PVP phases, and the crystallite size of the materials was determined to be in the range of 33–43 nm. The structural analyses, FT-IR, and Raman spectroscopy proved the interactions between the perovskite and the polymer, which led to novel composite materials. The different methods used for the synthesis of the new materials influenced their features and behavior. Moreover, the composites were successfully tested for methyl orange (MO) elimination from an acidic aqueous solution in dark conditions, with fast and complete (>95%) MO degradation at pH = 2.
Probabilistic Modelling of Fatigue Behaviour of 51CrV4 Steel for Railway Parabolic Leaf Springs
Authors: Gomes Vítor M. G. , Fiorentin Felipe K. , Dantas Rita , Silva Filipe G. A. , Correia José A. F. O. , de Jesus Abílio M. P.
Published: February 2025

The longevity of railway vehicles is an important factor in their mechanical and structural design. Fatigue is a major issue that affects the durability of railway components, and therefore, knowledge of the fatigue resistance characteristics of critical components, such as the leaf springs, must be extensively investigated. This research covers the fatigue resistance of chromium–vanadium alloy steel, usually designated as 51CrV4, from the high-cycle regime (HCF) (103–104) up to very high-cycle fatigue (VHCF) (109) under the bending loading conditions typical of leaf springs and under uniaxial tension/compression loading, respectively, for a stress ratio, Rσ, of −1. Different test frequencies were considered (23, 150, and 20,000 Hz) despite the material not exhibiting a relatively significant frequency effect. In order to create a new fatigue prediction model, two prediction models, the Basquin SN linear regression model and the Castillo–Fernandez–Cantelli (CFC) model, were evaluated. According to the analysis carried out, the CFC model provided a better prediction of the fatigue failures than the SN model, especially when outlier failure data were considered. The investigation also examined the failure modes, observing multiple cracks for higher loads and single cracks that initiated on the surface or from internal inclusions at lower loading. The present investigation aims to provide a fatigue resistance prediction model encompassing the HCF and VHCF regions for the fatigue design of railway wagon leaf springs, or even for other components made of 51CrV4 with a tempered martensitic microstructure.
Low-Cost Direct Digital Synthesis-Based On-Chip Waveform Generation for Analog/Mixed Signal BIST Applications
Authors: Nti Darko Emmanuel , Bruce Isaac , Oko-Odion Ekaniyere , Bhatheja Kushagra , Karimpour Saeid , Chen Degang
Published: February 2025

Waveform generation as part of on-chip built-in self-test (BIST) circuitry often necessitates sufficient linearity without expensive hardware overhead. Achieving high linearity is critical for accurate signal generation, especially in applications requiring high precision, such as biomedical and instrumentation applications. Currently, achieving the high linearity and precision required in signal generators often relies on costly hardware such as automated test equipment (ATE). This paper presents a DAC-based arbitrary waveform generator (AWG). We use a low-cost DAC and a fully digital on-chip testing and calibration approach to nullify the effect of the DAC’s non-linearity on the generated waveform. The ultra-low cost and high linearity benefit of the proposed waveform generator makes it highly suitable for integration into resource-constrained systems. The proposed approach is validated using simulation results of the small-area DAC designed in TSMC 0.18 μm technology and the testing and calibration algorithms implemented in MATLAB. The DAC, designed with a matching accuracy at only the 5-bit level, is able to generate a signal with an ENOB of 12 bits alongside an SFDR and THD surpassing 100 dB. This high level of signal purity is consistently maintained across 100 Monte Carlo simulations, demonstrating the robustness of the architecture against PVT variations as well as random mismatches.
Analysis of Measurement Uncertainty of Natural Source Zone Depletion Rate of Hydrocarbons as a Function of the Uncertainty of Subsurface Temperature
Authors: Ludwig Guilherme H. , Cervelin Leonardo N. , Pacheco Antonio L. S. , Flesch Rodolfo C. C.
Published: February 2025

Environmental monitoring and remediation of hydrocarbon contamination in soil, particularly through passive methods, has become a critical area of focus for oil companies adapting to current environmental standards. Natural source zone depletion (NSZD) is a passive remediation process in which the degradation of petroleum hydrocarbons by microorganisms produces measurable thermal effects on the subsurface. Accurate estimation of the NSZD rate is heavily dependent on the precision of temperature measurements, as small uncertainties in temperature can cause significant variations in the estimated rates. Despite growing interest in using subsurface temperature data for NSZD monitoring, there is a lack of studies addressing the impact of temperature measurement uncertainty on the reliability of depletion rate estimates. This paper proposes a Monte Carlo method approach to assess the propagation of temperature measurement uncertainties through the NSZD rate estimation process. By simulating different uncertainty scenarios, this work defines acceptable limits for temperature measurement errors to ensure accurate and representative NSZD rate calculations. For the analyzed case study, it was determined that the relationship between uncertainties was nearly linear, with a slope of 52.5 L m−2 year−1 in the estimated NSZD rate for each degree Celsius of uncertainty in the temperature measurements.
Fano Resonant Sensing in MIM Waveguide Structures Based on Multiple Circular Split-Ring Resonant Cavities
Authors: Wang Wenjing , Zhang Shaoze , Cao Huiliang
Published: February 2025

In this work, a non-through metal–insulator–metal (MIM) waveguide capable of exciting three Fano resonances was designed and numerically studied using the finite element method. Fano resonances are achieved through the interaction between the modes of multiple circular split-ring resonator cavities and the waveguide. The effect of coupling between different resonators on the Fano resonance peaks is investigated. Independent tuning of the Fano resonance wavelength and transmission rate is accomplished by modifying the structural rotation angle and geometric parameters. After optimizing these parameters, the structure achieves an optimal refractive index sensitivity of 946.88 nm/RIU and a figure of merit of 99.17. The proposed structure holds potential for guiding the design of nanosensors.
Effect of Carotenoid Composition on Stability and Light-Induced Oxidative Damage of the LH2 Complexes Isolated from Ectothiorhodospira haloalkaliphila
Authors: Yanykin Denis V. , Paskhin Mark O. , Shumeyko Sergey A. , Ashikhmin Aleksandr A. , Bolshakov Maxim A.
Published: February 2025

Earlier, it has been shown that carotenoid-dependent singlet oxygen photogeneration in LH2 of Ectothiorhodospira haloalkaliphila leads to damage to pigments and protein. Present work continues this investigation using LH2 complexes with altered carotenoid composition: carotenoid-less LH2, and LH2 complexes with incorporated neurosporene, spheroidene, or rhodopin (LH2-Neu, LH2-Sph, or LH2-Rho, respectively). This work provides the first data on the products (hydroperoxides of organic molecules, most likely components of the protein matrix of the complexes) of the interaction of singlet oxygen with LH2 components with a modified carotenoid composition; the ability of various carotenoids to both influence the stability of LH2 and participate in oxidative damage to the complexes is assessed. It was shown that inhibition of carotenoid synthesis led to a decrease in LH2 thermal stability and reduced the light-induced oxidative damage to bacteriochlorophyll and protein. Re-incorporation of exogenous carotenoids did not return stability of the complexes but reduced the tendency of complexes to aggregate, and (in the case of LH2-Rho) reactivated both photooxidation of bacteriochlorophyll and photoproduction of organic hydroperoxides. It was concluded that carotenoids play an important role in comple x stability and are capable of inducing oxidative damage to LH2 components through singlet oxygen photogeneration.
Production and Bioseparation Applications of Polyhydroxyalkanoate Nano-Granules Functionalized with Streptavidin
Authors: Zhang Yuyan , Zhao Jiping , Guo Hui , Lu Xiaoyun , Tan Dan
Published: February 2025

Rapidly growing industrial biotechnology and bio-manufacturing require simple and cost-effective bioseparation tools. A novel strategy of bioseparation based on the streptavidin-decorated polyhydroxyalkanoate (PHA) nano-granules was developed in this study. By fusing to the N-terminus of PHA-associated phasin protein, the streptavidin was one-step immobilized on the surface of PHA nano-granules simultaneously with the accumulation of PHA in recombinant Escherichia coli. About 1.95 g/L of PHA nano-granules (54.51 wt% of cell dry weight) were produced after 48 h bacterial cultivation. The following qualitative and quantitative characterizations demonstrated that the streptavidin accounted for approximately 6.78% of the total weight of the purified PHA nano-granules and confirmed a considerable biotin affinity of 0.1 ng biotin/μg surface protein. As a proof of concept, the nano-granules were further functionalized with biotinylated oligo(dT) for mRNA isolation and about 1.26 μg of mRNA (occupied 2.59%) was purified from 48.45 μg of total RNA, achieving good integrity and high purity with few DNA and rRNA contaminations. Moreover, the nano-granules retained more than 80% of their initial mRNA recovery efficiency after ten cycles of repeated use. The PHA-SAP nano-granules were also functionalized with biotinylated magnetic beads, allowing magnetic recovery of the PHA nano-granules from cell lysates that still needs optimization. Our study provides a novel and expandable platform of PHA nano-granules that can be further functionalized with various biological groups for bioseparation applications. The functional PHA nano-granules have a great potential to serve as bioseparation resin for large-scale purification processes after suitable optimizations for “bench-to-factory” translation, contributing to scalable and sustainable bioprocessing.
The Urogenital System Microbiota: Is It a New Gamechanger in Urogenital Cancers?
Authors: Ece Gülfem , Aktaş Ahmet , Caner Ayse , Sağlık İmran , Kula Atik Tuğba , Ulusan Bağcı Özlem , Bayındır Bilman Fulya , Demirbakan Hadiye , Güdül Havuz Seda , Kaya Esra , Koyuncu Özyurt Özlem , Yetkin Gülay , Zorbozan Orçun
Published: February 2025

The human microbiome, which encompasses microbial communities and their genetic material, significantly influences health and disease, including cancer. The urogenital microbiota, naturally present in the urinary and genital tracts, interact with factors such as age, lifestyle, and health conditions to affect homeostasis and carcinogenesis. Studies suggest that alterations in this microbiota contribute to the development and progression of genitourinary cancers, emphasizing the concept of oncobiome, which refers to microbial genetic contributions to cancer. Similarly, gut microbiota can influence hormone levels and systemic inflammation, impacting cancers such as cervical and prostate cancer. Advanced studies indicate that microbial communities in genitourinary cancers have distinct profiles that may serve as diagnostic biomarkers or therapeutic targets. Dysbiosis of the urinary microbiota correlates with bladder and kidney cancer. Additionally, gut microbiota influence the effectiveness of cancer treatments. However, further research is necessary to clarify causality, the role of microbial metabolites, and hormonal regulation. The aim of this review is to understand that these dynamics present opportunities for innovative cancer diagnostics and therapies, highlighting the need for integration of microbiology, oncology, and genomics to explore the role of microbiota in genitourinary cancers. For this, a comprehensive search of relevant databases was conducted, applying specific inclusion and exclusion criteria to identify studies examining the association between microbiota and urogenital cancers. Research into the mechanisms by which microbiota influence urogenital cancers may pave the way for new diagnostic and therapeutic approaches, ultimately improving patient outcomes.
Mechanisms Driving Seasonal Succession and Community Assembly in Sediment Microbial Communities Across the Dali River Basin, the Loess Plateau, China
Authors: Chen Xin , Li Jing , Xu Guoce , Fang Kang , Wan Shun , Wang Bin , Gu Fengyou
Published: February 2025

Microorganisms are instrumental in river ecosystems and participate in biogeochemical cycles. It is thought that dynamic hydrological processes in rivers influence microbial community assembly, but the seasonal succession and community assembly of river sediments on the Loess Plateau remain unclear. This study used high-throughput sequencing technology (16S and ITS) and the neutral community model to analyze seasonal succession and the assembly processes associated with microbial communities in the Dali River, a tributary of the Yellow River on the Loess Plateau. The results showed that sediment bacterial and fungal community diversity indexes in non-flood season were 1.03–3.15 times greater than those in flood season. There were obvious variations between non-flood and flood seasons in sediment microorganisms. The similarities among all, abundant, and rare microbial communities decreased as geographical distance increased. Proteobacteria (52.5–99.6%) and Ascomycota (22.0–34.2%) were the primary microbial phyla in all, abundant, and rare microbial communities. Sediment ammonia nitrogen, water temperature, and sediment organic carbon significantly affected (p < 0.05) the structure of all, abundant, and rare sediment microorganism communities. The ecological networks for the bacterial community of non-flood season and fungal community of flood season had complex topological parameters. The bacterial community in river sediments was driven by deterministic processes, while the fungal community was dominated by stochastic processes. These results expanded understanding about sediment microbial community characteristics in rivers on the Loess Plateau and provided insights into the assembly processes and the factors driving microbial communities in river networks.
Drug Resistance Mutations (DRMs) for Long-Acting Injectable Cabotegravir and Rilpivirine (CAB/RPV LAI) in the HIV-1 Subtype A6 Epidemic in Poland
Authors: Załęski Andrzej , Lembas Agnieszka , Dyda Tomasz , Osińska Joanna , Jabłońska Joanna , Stempkowska-Rejek Justyna , Orzechowska Justyna , Wiercińska-Drapało Alicja
Published: February 2025

HIV subtype A6 with the L74I polymorphism, which increases the risk of cabotegravir/rilpivirine treatment failure, causes more and more infections in Poland. In this multicenter, observational, cross-sectional study (2023–2024), we analyzed viral subtypes and drug-resistance mutations to drugs used for long-acting injectable antiretroviral treatment and pre-exposure prophylaxis. Among 357 people with HIV, 247 (69%) were Polish nationals, and 102 (29%) were from former Soviet Union countries. Of the 357 people included, 159 (45%) had subtype B, and 177 (50%) had subtype A6 infections, with 165 (87%) of the latter characterized by the L74I polymorphism. Subtype A6 was more frequent in women (66% vs. 46% in men, p < 0.05) and among people from former Soviet countries (77% vs. 39% in Polish nationals, p < 0.05). About 40% of people had either drug-resistance mutations for cabotegravir/rilpivirine or HIV A6 subtype with the L74I polymorphism; 4.5% had both of these conditions. Compared to subtype B infections, subtype A6 infections were characterized by more frequent major transmitted drug-resistance mutations for non-nucleoside reverse transcriptase inhibitors (8.5% vs. 1.9%, p = 0.007) and rilpivirine (5.1% vs. 0.6%, p = 0.016). Due to the frequent occurrence of the L74I polymorphism and drug-resistance mutations in HIV A6 subtype infection, about 40% of people with HIV in Poland may be at risk of long-acting injectable treatment failure.
Antimicrobial Potential of Cannabinoids: A Scoping Review of the Past 5 Years
Authors: Coelho Maria João , Araújo Maria Duarte , Carvalho Márcia , Cardoso Inês Lopes , Manso Maria Conceição , Pina Cristina
Published: February 2025

In the scenario of fighting bacterial resistance to antibiotics, natural products have been extensively investigated for their potential antibacterial activities. Among these, cannabinoids—bioactive compounds derived from cannabis—have garnered attention for their diverse biological activities, including anxiolytic, anti-inflammatory, analgesic, antioxidant, and neuroprotective properties. Emerging evidence suggests that cannabinoids may also possess significant antimicrobial properties, with potential applications in enhancing the efficacy of conventional antimicrobial agents. Therefore, this review examines evidence from the past five years on the antimicrobial properties of cannabinoids, focusing on underlying mechanisms such as microbial membrane disruption, immune response modulation, and interference with microbial virulence factors. In addition, their synergistic potential, when used alongside standard therapies, underscores their promise as a novel strategy to address drug resistance, although further research and clinical trials are needed to validate their therapeutic use. Overall, cannabinoids offer a promising avenue for the development of innovative treatments to combat drug-resistant infections and reduce the reliance on traditional antimicrobial agents.
Photo(electro)catalytic Water Splitting for Hydrogen Production: Mechanism, Design, Optimization, and Economy
Authors: Li Xingpeng , Zhang Chenxi , Geng Jiafeng , Zong Shichao , Wang Pengqian
Published: February 2025

As an energy carrier characterized by its high energy density and eco-friendliness, hydrogen holds a pivotal position in energy transition. This paper elaborates on the scientific foundations and recent progress of photo- and electro-catalytic water splitting, including the corresponding mechanism, material design and optimization, and the economy of hydrogen production. It systematically reviews the research progress in photo(electro)catalytic materials, including oxides, sulfides, nitrides, noble metals, non-noble metal, and some novel photocatalysts and provides an in-depth analysis of strategies for optimizing these materials through material design, component adjustment, and surface modification. In particular, it is pointed out that nanostructure regulation, dimensional engineering, defect introduction, doping, alloying, and surface functionalization can remarkably improve the catalyst performance. The importance of adjusting reaction conditions, such as pH and the addition of sacrificial agents, to boost catalytic efficiency is also discussed, along with a comparison of the cost-effectiveness of different hydrogen production technologies. Despite the significant scientific advancements made in photo(electro)catalytic water splitting technology, this paper also highlights the challenges faced by this field, including the development of more efficient and stable photo(electro)catalysts, the improvement of system energy conversion efficiency, cost reduction, the promotion of technology industrialization, and addressing environmental issues.
Main Chemical Components, Activity and Mechanism of Repellence of Cyperus esculentus Essential Oil Against Tribolium confusum
Authors: Feng Xu , Shan Cheng-Bin , Ma Jian-Nan , Ma Yue , Li Na , Zhang De-Jian , Lu Zhan-Yuan , Ma Chao-Mei
Published: February 2025

Tribolium confusum is a major stored-product pest that exhibits resistance to chemically synthesized pest repellents. This study investigated the potential of essential oil (EO) extracted from the roots of Cyperus esculentus as a natural alternative for pest management. The EO was obtained through steam distillation, and its chemical composition was elucidated using gas chromatography–mass spectrometry. The primary compounds, cyperotundone and cyperene, were further isolated from the EO through silica gel column chromatography. The efficacy of the EO and its isolated compounds as pest repellents was evaluated against a flaxseed pest, which was identified as T. confusum through DNA sequence analysis. The results demonstrated that at 86.12 μg/cm2, the EO and its two main components maintained significant repellent activity for up to 24 h. In contrast, the effectiveness of the positive control, N, N-diethyl-3-methylbenzamide (DEET) declined rapidly after 8 h. At 16 h, the repellent activity of the EO and one of its main components, cyperotundone, was significantly greater than that of DEET. Furthermore, at a lower concentration of 43.06 μg/cm2, cyperotundone’s repellent activity was significantly stronger than DEET’s at 16 h. Additionally, cyperotundone outperformed DEET significantly from 4 to 16 h at 21.53 μg/cm2 and at 16 h at 10.76 μg/cm2. Among the two compounds, cyperotundone exhibited a longer-lasting repellent effect compared to cyperene, which is consistent with the lower evaporation rate of cyperotundone. Biochemical assays revealed that exposure to the EO of C. esculentus and its major compounds significantly reduced (p < 0.05) the activities of acetylcholinesterase and glutathione-S-transferase in T. confusum. Molecular docking experiments indicated that the compounds could bind to olfactory receptors with low binding energies. qRT-PCR analysis revealed that the EO and its two compounds significantly altered (p < 0.05) the expression levels of odorant receptor genes in the pest. These findings suggest that the repellent action of C. esculentus EO and its major compounds on T. confusum may be mediated through the modulation of the pest’s olfactory system, as well as by inhibiting essential enzymatic activities in the pests. This research contributes valuable insights into the development of sustainable, long-lasting, and eco-friendly pest repellents, harnessing the potential of the rich botanical resource C. esculentus.
Impact of Activation Conditions on the Electrochemical Performance of Rice Straw Biochar for Supercapacitor Electrodes
Authors: Cheng Jialuo , Lu Yumeng , Sun Ya , Deng Sunhua , Yang Heng , Zhang Manman , Wang Chunlei , Yan Juntao
Published: February 2025

Biochar, derived from agricultural waste, has gained significant attention as a sustainable material for energy storage applications due to its high surface area, tunable porosity, and environmental benefits. This study investigates the electrochemical performance of rice straw biochar (RSBC) as an electrode material, with a focus on the effects of activation temperature, activation ratio, and activation time. Among the prepared samples, RSBC-2, activated at 800 °C with a 1:2 KOH ratio for 0.5 h, exhibited the best electrochemical performance. Characterization of RSBC and RSBC-2 showed significant improvements in surface area and pore structure. Specifically, the BET surface area of RSBC-2 increased to 939.40 m2 g−1, with a reduced average pore size of 2.27 nm. Electrochemical testing revealed that RSBC-2 achieved specific capacitances of 296, 281, 272, 260, and 240 F g−1 at current densities of 0.2, 0.5, 1, 2, and 5 A g−1, respectively, with a capacity retention rate of 81%. The improved electrochemical performance of RSBC-2 is attributed to its larger surface area and enhanced pore structure, which facilitate better charge storage and overall electrochemical behavior, making it a promising candidate for energy storage applications.
The High-Pressure Response of 9,9′-Spirobifluorene Studied by Raman Spectroscopy
Authors: Siavou Maria-Tereza , Siapaka Konstantina , Karabinaki Olga , Christofilos Dimitrios , Arvanitidis John
Published: February 2025

The pressure response of crystalline 9,9′-spirobifluorene up to 8 GPa was studied by means of Raman spectroscopy using a diamond anvil cell as a pressure chamber. With increasing pressure, the observed Raman peaks shifted to higher frequencies, reflecting the bond hardening upon volume reduction, which was much more pronounced for the initially weaker intermolecular interactions than for the stronger intramolecular covalent bonds. The significant changes in the Raman spectrum and the pressure evolution of the frequencies at ~1.3 GPa for both the intermolecular and the intramolecular Raman peaks signaled a pressure-induced structural and molecular conformation transition with a little hysteretic behavior (~0.5 GPa) upon pressure release. For P > 4 GPa, the reversible decrease of the pressure coefficients of the majority of the intermolecular and some intramolecular peak frequencies indicated another structural modification of the studied molecular crystal. A value of ~9 GPa for the bulk modulus of the system at zero pressure was estimated from the logarithmic pressure coefficients of the frequencies of the intermolecular modes in the low-pressure phase. These coefficients were reduced by ~6 times at 4.2 GPa, indicating that the considerable stiffening of the material in the high-pressure phase emanated from the selective strengthening of the intermolecular interactions.
Molecular Network Polyamorphism in Mechanically Activated Arsenic Selenides Under Deviation from As2Se3 Stoichiometry
Authors: Shpotyuk Oleh , Lukáčová Bujňáková Zdenka , Baláž Peter , Shpotyuk Yaroslav , Hyla Malgorzata , Kozdras Andrzej , Ingram Adam , Boyko Vitaliy , Demchenko Pavlo , Kovalskiy Andriy
Published: February 2025

Polyamorphic transitions driven by high-energy mechanical milling (nanomilling) are studied in thioarsenide As4Sen-type glassy alloys obtained by melt quenching deviated from arsenic triselenide As2Se3 stoichiometry towards tetraarsenic pentaselenide (g-As4Se5) and tetraarsenic tetraselenide (g-As4Se4). This employs a multiexperimental approach based on powder X-ray diffraction (XRD) analysis complemented by thermophysical heat transfer, micro-Raman scattering (micro-RS) spectroscopy, and revised positron annihilation lifetime (PAL) analysis. Microstructure scenarios of these nanomilling-driven transformations in arsenoselenides are identified by quantum-chemical modeling using the authorized modeling code CINCA (the Cation Interlinked Network Cluster Approach). A straightforward interpretation of a medium-range structure response of a nanomilling-driven polyamorphism in the arsenoselenides is developed within the modified microcrystalline model. Within this model, the diffuse peak-halos arrangement in the XRD patterning is treated as a superposition of the Bragg-diffraction contribution from inter-planar correlations supplemented by the Ehrenfest-diffraction contribution from inter-atomic (inter-molecular) correlations related to derivatives of network As2Se3-type and molecular As4Se4-type conformations. Changes in the medium-range structure of examined glassy arsenoselenides subjected to nanomilling occur as an interplay between disrupted intermediate-range ordering and enhanced extended-range ordering. The domination of network-forming conformations in arsenoselenides deviated from As2Se3 stoichiometry (such as g-As4Se5) results in rather slight changes in their calorimetric heat-transfer and micro-RS responses. At the atomic-deficient level probed by PAL spectroscopy, these changes are accompanied by reduced positron trapping rate of agglomerated multiatomic vacancies and vacancy-type clusters in an amorphous As-Se network. Under an increase in As content beyond the g-As4Se5 composition approaching g-As4Se4, nanomilling-driven polyamorphic transitions, which can be classified as reamorphization (amorphous I-to-amorphous II) phase transitions, are essentially enhanced due to the higher molecularity of these glassy alloys enriched in thioarsenide-type As4Se4 cage-like molecular entities and their low-order network-forming derivatives.
Impact of Bioactive Ingredients on the Fecal Excretion of Aflatoxin B1 and Ochratoxin A in Wistar Rats
Authors: Vila-Donat Pilar , Sánchez Dora , Manyes Lara , Cimbalo Alessandra
Published: February 2025

This study evaluates the effects of fermented whey (FW) and pumpkin (P) on the excretion of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in rats using immunoaffinity column cleanup and high-performance liquid chromatography–fluorescence detection (IAC-LC-FLD). The method achieved detection limits of 0.1 µg/kg for AFB1 and 0.3 µg/kg for OTA, with recovery rates ranging from 72–92% for AFB1 and 88–98% for OTA. A fecal analysis of 100 rats showed peak AFB1 concentrations of 418 µg/kg and OTA of 1729 µg/kg. In the toxin-exposed groups, OTA levels were higher than AFB1, with males in the OTA-only group showing significantly higher OTA (1729 ± 712 µg/kg) than females (933 ± 512 µg/kg). In the AFB1-only group, the fecal levels were 52 ± 61 µg/kg in males and 91 ± 77 µg/kg in females. The AFB1 + FW group showed notable AFB1 concentrations (211 ± 51 µg/kg in males, 230 ± 36 µg/kg in females). The FW + P combination further influenced excretion, with higher AFB1 and OTA levels. These findings suggest that FW and P modulate mycotoxin excretion and may play a role in mycotoxin detoxification, providing insight into dietary strategies to reduce mycotoxin exposure and its harmful effects.
Charge Regulation in Liquid Films Stabilized by Ionic Surfactants: Change in Adsorption with Film Thickness and Phase Transitions
Authors: Dimitrova Iglika M. , Slavchov Radomir I.
Published: February 2025

When a liquid film is thinning, the charge and the potential of its surfaces change simultaneously due to the interaction between the two surfaces. This phenomenon is an example for charge regulation and has been known for half a century for systems featuring aqueous solutions in contact with metals, salts, biological surfaces covered by protolytes, etc. Few studies, however, investigated regulation in foam and emulsion films, where the charge is carried by soluble ionic surfactants. This work presents an analysis of the phenomenon for surfactants that follow the classical Davies adsorption isotherm. The electrostatic disjoining pressure Πel was analyzed, and the Davies isotherm was shown to lead to Πel ∝ h−1/2 behavior at a small film thickness h. As usual, the charge regulation regime (constant chemical potential of the surfactant) corresponded to a dependence of Πel on h between those for constant charge and constant electric potential regimes. The role of the background electrolyte was also studied. At the water–air interface, many ionic surfactants exhibit a surface phase transition. We show that the interaction between the two surfaces of a foam film can trigger the phase transition (i.e., the film changes its charge abruptly), and two films of different h values can coexist in equilibrium with each other—one covered by surfactant in the 2D gaseous state and another in the 2D liquid state.
Small Molecules from Medicinal Plant Iris tectorum as Histidine Kinase Inhibitor to Resensitize β-Lactam-Resistant Escherichia coli
Authors: Ji Youqi , Wang Yinhuan , Xu Zhangkai , Chen Danlei , Yu Zhendi , Shao Qingyi , Hong Xin , Liu Zishu , Cheng Dongqing
Published: February 2025

Background: Due to the widespread use of broad-spectrum antibiotics, the problem of antibiotic resistance has become an increasingly serious global threat. One of the key mechanisms of Escherichia coli resistance to beta-lactam antibiotics is the production of beta-lactamase enzymes, which poses a dilemma for clinicians in selecting antibiotics when faced with resistant bacterial infections. However, research on the reversal of bacterial resistance is limited. Methods: This study involved the preparation of Iris tectorum extract and detection of its effects on antibiotics sensitivity, extended-spectrum beta-lactamase (ESBL) gene expression, and histidine kinase phosphorylation levels in β-lactam antibiotic-resistant Escherichia coli. Additionally, analyses of the active ingredients of Iris tectorum extract were conducted with a liquid chromatography–mass spectrometer, and the binding sites were predicted by molecular docking. Results: Iris tectorum extract could restore the sensitivity of Escherichia coli to beta-lactam antibiotics and reduce the expression levels of ESBL genes and histidine phosphorylation levels. The active ingredients of Iris tectorum extract may be irigenin and tectorigenin, and these two small molecules could bind to histidine kinase to inhibit phosphorylation. Conclusions: Iris tectorum extract may serve as an antibiotic adjuvant, restoring the sensitivity of antibiotic-resistant bacteria by inhibiting histidine kinase phosphorylation, thereby alleviating the problem of Escherichia coli resistance to β-lactam antibiotics.
Oxazolo[5,4-d]pyrimidines as Anticancer Agents: A Comprehensive Review of the Literature Focusing on SAR Analysis
Authors: Sochacka-Ćwikła Aleksandra , Mączyński Marcin
Published: February 2025

Oxazolo[5,4-d]pyrimidines have been found to exhibit a wide range of biological activities, including the inhibition of various enzymes and signaling pathways associated with carcinogenesis. The objective of this review is to demonstrate that the oxazolo[5,4-d]pyrimidine scaffold represents a valuable structure for the design of novel anticancer therapies. The article provides a comprehensive overview of the chemical structure and pharmacological properties of oxazolo[5,4-d]pyrimidine derivatives, drawing upon the literature and international patents from 1974 until the present. Notably, the review explores structure–activity relationships (SAR) with a view to enhancing the therapeutic efficacy of oxazolo[5,4-d]pyrimidines.
Synthesis and Characterization of Lignocellulose-Based Carbon Quantum Dots (CQDs) and Their Antimicrobial and Antioxidant Functionalities
Authors: Lee Wooseok , Ko Seonghyuk
Published: February 2025

Carbon quantum dots (CQDs) have recently drawn enormous attention due to not only their unique chemical, biological, and optical properties but also because a variety of renewable biomasses are readily utilized as carbon sources in their synthesis. This study investigated the synthesis, characterization, and functional evaluation of CQDs from unbleached mechanical pulp as a natural lignocellulosic resource. The CQDs were synthesized using a one-step hydrothermal synthesis with varying temperature, time, and pulp consistency. The resulting CQDs exhibit a spherical shape with a size distribution of 9.73 ± 0.82 nm and lattice parameters of 0.21 and 0.34 nm, indicating a graphite core. The photoluminescence spectra showed evident fluorescence characteristics, with an emission peak at 435 nm at an excitation wavelength of 370 nm. The as-prepared CQDs were also chemically composed of C=C and C=O bonds linked to the hydroxyl and carboxyl functional groups, which are typically found in lignocellulose-based CQDs. The CQDs demonstrated antibacterial activity exceeding 99.9% against E. coli at the lowest concentration of 0.75 mg/mL. Demonstrating its antioxidation property, the DPPH radical scavenging activity surpassed 90% with more than 40 µg/mL of the CQD solution.
Procurement, Processing, and Storage of Human Amniotic Membranes for Implantation Purposes in Non-Healing Pressure Ulcers
Authors: Gómez Lina A. , Domínguez-Paz Carlos , Ospina Juan F. , Vargas Elga J.
Published: February 2025

The human amniotic membrane (hAM) has been used as an implant to enhance the regenerative process and control inflammation in different diseases, given their structure, biocompatibility, presence of stem cells and multiple growth factors. The objective of this study was to generate a standardized protocol for obtaining, processing, and storing hAMs that guarantee the conservation of their structural and cellular characteristics as well as their mechanical properties, ensuring their ease of handling, sterility, and quality that allows their implementation for therapeutic purposes in the field of regenerative medicine. The hAMs were obtained from mothers with healthy, full-term, controlled pregnancies and by cesarean section. The hAMs were processed under sterile conditions, manually separated from the placenta and, subsequently, they were frozen in a solution of culture medium plus 50% v/v glycerol. The protocol allows obtaining sterile hAMs composed of both epithelium and stroma with adequate preservation of the amniotic cells. The glycerol’s impact on the mechanical properties may enhance the membrane’s adaptability and conformability to diverse wound surfaces, potentially improving the healing process. It is necessary to repeat microbiological, cell viability and mechanical studies at 6 and 12 months to ensure that long-term frozen conditions do not affect the quality of the hAMs.
Differential Expression of miRNAs Between Young-Onset and Late-Onset Indian Colorectal Carcinoma Patients
Authors: Moiz Sumaiya , Saha Barsha , Mondal Varsha , Bishnu Debarati , Das Biswajit , Bose Bodhisattva , Das Soumen , Banerjee Nirmalya , Dutta Amitava , Chatterjee Krishti , Goswami Srikanta , Mukhopadhyay Soma , Basu Sudarshana
Published: February 2025

Reports indicate a worldwide increase in the incidence of Early-Onset Colorectal Carcinoma (EOCRC) (<50 years old). In an effort to understand the different modes of pathogenesis in early-onset CRC, colorectal tumors from EOCRC (<50 years old) and Late-Onset patients (LOCRC; >50 years old) were screened to eliminate microsatellite instability (MSI), nuclear β-catenin, and APC mutations, as these are known canonical factors in CRC pathogenesis. Small-RNA sequencing followed by comparative analysis revealed differential expression of 23 miRNAs (microRNAs) specific to EOCRC and 11 miRNAs specific to LOCRC. We validated the top 10 EOCRC DEMs in TCGA-COAD and TCGA-READ cohorts, followed by validation in additional EOCRC and LOCRC cohorts. Our integrated analysis revealed upregulation of hsa-miR-1247-3p and hsa-miR-148a-3p and downregulation of hsa-miR-326 between the two subsets. Experimentally validated targets of the above miRNAs were compared with differentially expressed genes in the TCGA dataset to identify targets with physiological significance in EOCRC development. Our analysis revealed metabolic reprogramming, downregulation of anoikis-regulating pathways, and changes in tissue morphogenesis, potentially leading to anchorage-independent growth and progression of epithelial-mesenchymal transition (EMT). Upregulated targets include proteins present in the basal part of intestinal epithelial cells and genes whose expression is known to correlate with invasion and poor prognosis.
The Antiglioma Potential of Plant Lectins: Molecular Targets, Mechanisms, and Future Directions
Authors: Leal Rodrigo Bainy , Pinto-Junior Vanir Reis , Oliveira Messias Vital , Osterne Vinicius Jose Silva , Sartori Nicole , Santos Ana Carolina dos , Garcez Ricardo Castilho , Nascimento Kyria Santiago , Cavada Benildo Sousa
Published: February 2025

Gliomas, ranging from low-grade pilocytic astrocytomas to highly malignant glioblastomas, are primary brain tumors that originate from neural or glial stem cells. Classified by the WHO into grades 1 to 4, these tumors exhibit varying prognoses, with oligodendrogliomas and astrocytomas having better and intermediate outcomes, respectively, while glioblastomas are associated with a poor prognosis. Despite advancements in molecular and genetic research that have improved diagnosis and the development of targeted therapies, treating high-grade gliomas remains a significant challenge due to their diffuse nature. In this context, lectins, carbohydrate-binding proteins, have shown promise as diagnostic and therapeutic agents for cancer, including gliomas. Plant lectins, particularly those from legumes, exhibit significant antiproliferative effects on glioma cells. These effects include decreased cell viability and migration, alongside the induction of autophagy and apoptosis, suggesting their potential as therapeutic agents. Although the mechanisms underlying these effects are not yet fully understood, molecular targets and pathways involved in the antiglioma activity of lectins have been identified. Key targets include matrix metalloproteinases (MMPs), epidermal growth factor receptor (EGFR), CD98 (xc- system), AMPA receptor, and CD73. This review focuses on the antiglioma potential of legume lectins, their applications, and the main molecular targets based on their functions, structures, and associated molecular mechanisms.