bluuebunny/crossref_metadata_2025 huggingface dataset:
Organoleptic Chemical Markers of Serpa PDO Cheese Specificity
Authors: Araújo-Rodrigues Helena , Martins António P. L. , Tavaria Freni K. , Santos Maria Teresa G. , Carvalho Maria João , Dias João , Alvarenga Nuno B. , Pintado Manuela E.
Published: June 2022

Serpa is a protected designation of origin cheese produced with a vegetable coagulant (Cynara cardunculus L.) and raw ovine milk. Despite the unique sensory profile of raw milk cheeses, numerous parameters influence their sensory properties and safety. To protect the Serpa cheese quality and contribute to unifying their distinctive features, some rheologic and physicochemical parameters of cheeses from four PDO producers, in distinct seasons and with different sensory scores, were monitored. The results suggested a high chemical diversity and variation according to the dairy, month and season, which corroborates the significant heterogeneity. However, a higher incidence of some compounds was found: a group of free amino acids (Glu, Ala, Leu, Val and Phe), lactic and acetic acids, some volatile fatty acids (e.g., iC4, iC5, C6 and C12) and esters (e.g., ethyl butanoate, decanoate and dodecanoate). Through the successive statistical analysis, 13 variables were selected as chemical markers of Serpa cheese specificity: C3, C4, iC5, C12, Tyr, Trp, Ile, 2-undecanone, ethyl isovalerate, moisture content on a fat-free basis, the nitrogen-fractions (maturation index and non-protein and total nitrogen ratio) and G’ 1 Hz. These sensory markers’ identification will be essential to guide the selection and development of an autochthonous starter culture to improve cheese quality and safety issues and maintain some of the Serpa authenticity.
Finite Element Analysis and Near-Infrared Hyperspectral Reflectance Imaging for the Determination of Blueberry Bruise Grading
Authors: Zheng Zhaoqi , An Zimin , Liu Xinyu , Chen Jinghui , Wang Yonghong
Published: June 2022

Bruising of the subcutaneous tissues of blueberries is an important form of mechanical damage. Different levels of bruising have a significant effect on the post-harvest marketing of blueberries. To distinguish different grades of blueberry bruises and explore the effects of different factors, explicit dynamic simulation and near-infrared hyperspectral reflectance imaging were employed without harming the blueberries in this study. Based on the results of the compression experiment, an explicit dynamic simulation of blueberries was performed to measure the potential locations of bruises and preliminarily divide the bruise stages. A near-infrared hyperspectral reflectance imaging system was used to detect the actual blueberry bruises. According to the blueberry photos taken by the near-infrared hyperspectral reflectance imaging system, the actual bruise rates of blueberries were obtained by using the Environment for Visualizing Images software for training and classification. Bruise grades of blueberries were divided accordingly. Response surface methodology was used to determine the effects of ripeness, loading speed and loading location on the blueberry bruising rate. Under the optimized parameters, the actual damage rate of blueberries was 1.1%. The results provide an important theoretical basis for the accurate and rapid identification and classification of blueberry bruise damage.
Application of Quantitative Microbiology and Challenge Tests to Reach a Suggested Food Safety Objective in a Middle Eastern-Style Ready-to-Cook Chicken Product
Authors: Osaili Tareq M. , Giatrakou Vasiliki , Ntzimani Athina , Tsiraki Maria , Savvaidis Ioannis N.
Published: June 2022

The contamination of ready-to-eat (RTE) and ready-to-cook (RTC) food products is a major global issue raising worry to consumers. Therefore, the behavior of Listeria monocytogenes and Salmonella spp., inoculated on a traditional Middle Eastern (M.E.) ready-to-cook (RTC) chicken product (“Taouk”-style), using the Risk Ranger® tool and the necessary management options (to accomplish the hypothetical food safety objectives (FSO)), when unsuspecting consumers may taste such a product were the primary subjects of our study. The behavior of the aforementioned pathogens was studied in the presence and absence of a selected natural antimicrobial combination (chitosan [CH] and thyme oil [T]), and were added as a combined treatment (M-CH-T) to the RTs chicken samples, stored at 4 or 8 °C for a period of 8 d. In the product, wherein no antimicrobials were added (control treatment, M), the initial counts of L. monocytogenes increased by ca. 1.5 (4 °C) and 3.0 (8 °C) log colony-forming units (CFU)/g during an 8-d storage. Salmonella spp. numbers did not increase during storage at 4 °C in the non-treated product, but at 8 °C, an increase of ca. 2.5 log CFU/g occurred. Addition of CH in combination with T to the RTC product (M-CH-T) inhibited the growth of L. monocytogenes and produced lower counts of Salmonella at 4 °C. However, M-CH-T treatment was less effective against both pathogens compared to the control after the 6th day of storage (8 °C). Predictive models based on quantitative microbiology, combined with hazard identification applied in the present study, may be potential means of assessing the safety of the RTC chicken products. It must be noted that for warranting the food safety of especially perishable items (e.g., chicken products), an efficient food safety management system must be applied, in addition to testing of the finished product, (e.g., based on the HACCP principles).
Application of Protein in Extrusion-Based 3D Food Printing: Current Status and Prospectus
Authors: Guo Ziang , Arslan Muhammad , Li Zhihua , Cen Shaoyi , Shi Jiyong , Huang Xiaowei , Xiao Jianbo , Zou Xiaobo
Published: June 2022

Extrusion-based 3D food printing is one of the most common ways to manufacture complex shapes and personalized food. A wide variety of food raw materials have been documented in the last two decades for the fabrication of personalized food for various groups of people. This review aims to highlight the most relevant and current information on the use of protein raw materials as functional 3D food printing ink. The functional properties of protein raw materials, influencing factors, and application of different types of protein in 3D food printing were also discussed. This article also clarified that the effective and reasonable utilization of protein is a vital part of the future 3D food printing ink development process. The challenges of achieving comprehensive nutrition and customization, enhancing printing precision and accuracy, and paying attention to product appearance, texture, and shelf life remain significant.
E. tapos Yoghurt—A View from Nutritional Composition and Toxicological Evaluation
Authors: Naomi Ruth , Rusli Rusydatul Nabila Mahmad , Balan Santhra Segaran , Othman Fezah , Jasni Azmiza Syawani , Jumidil Siti Hadizah , Bahari Hasnah , Yazid Muhammad Dain
Published: June 2022

Elateriospermum tapos (E. tapos) is a natural tropical plant that possess a wide range of health benefits. Recent discovery proves that E. tapos extract is able to reduce weight, increase cognitive performance, and ameliorate anxiety and stress hormone. However, this extraction has not been incorporated into yoghurt, and no toxicity studies have been done previously to prove its safety. Thus, this study was aimed to formulate the ethanolic extracted E. tapos into yoghurt and access the toxicological effects on rodents. Forty female Sprague Dawley (SD) rats were used in this study and force fed with either one of the following doses of 250, 500, 1000, or 2000 mg/kg, while the control group received normal saline. The nutritional analysis result showed that the newly formulated yoghurt comprised 328 kJ of energy per 100 mL of servings, 3.6 g of fats, 8.2 g of carbohydrates, 2.7 g of total protein, and 1.2 g of fibre. The peak intensity of Lactobacillus species was observed at 1.6 × 105 CFU/g with a titratable acidity as lactic acid of 0.432 CFU/g, indicating the ability of the formulated yoghurt in stimulating the growth of Lactobacilli. In the experimental study, the E. tapos yoghurt in a single dose (2000 mg/kg) did not show any treatment related to toxicity in any of the rats observed in an additional 14 days. There were no changes in body weight, food and water intake, plasma biochemistry (ALT, AST, ALP, and creatinine), haematological products, and organ weights of the treated groups compared to the subacute control groups. Histological examination of all organs including liver, heart, and kidney were comparable to the control groups. In toto, oral consumptions of E. tapos yoghurt did not induce any adverse effects on rodents.
Relationship between Microbial Composition of Sourdough and Texture, Volatile Compounds of Chinese Steamed Bread
Authors: Fu Lili , Nowak Adriana , Zhao Hongfei , Zhang Bolin
Published: June 2022

The objective of this work was to explore the relationship between the microbial communities of sourdoughs collected from the Xinjiang and Gansu areas of China and the quality of steamed bread. Compared to yeast-based steamed bread, sourdough-based steamed bread is superior in terms of its hardness, adhesiveness, flexibility, and chewiness. It is rich in flavor compounds, but a significant difference in volatile flavor substances was observed between the two sourdoughs. A total of 19 strains of lactic acid bacteria (LAB) were isolated from the Gansu sourdough sample, in which Lactiplantibacillus plantarum and Pediococcus pentosaceus were the dominant species, accounting for 42.11% and 36.84%, respectively. A total of 16 strains of LAB were isolated from the Xinjiang sourdough sample, in which Lactiplantibacillus plantarum was the dominant species, accounting for 75%. High-throughput sequencing further confirmed these results. Clearly, the species diversity of Gansu sourdough was higher. The volatile profiles of the sourdoughs were similar, but differences in the individual volatile compounds were detected between the sourdoughs of the Gansu and Xinjiang regions. These results point out that the differences in the microbiota and the dominant strains lead to differences in the quality of sourdoughs from region to region. This investigation offers promising guidance on improving the quality of traditional steamed bread by adjusting the microorganisms in sourdough.
Fabrication of Dragee Containing Spirulina platensis Microalgae to Enrich Corn Snack and Evaluate Its Sensorial, Physicochemical and Nutritional Properties
Authors: Bayat Tork Maryam , Vazifedoost Mohsen , Hesarinejad Mohammad Ali , Didar Zohreh , Shafafi Zenoozian Masoud
Published: June 2022

In this work, the possibility of enriching snacks with Spirulina palatensis (SP) powder as a dragee was studied. In dragee formulation, the effects of various levels of SP, sunflower oil, NaCl and sour whey powder on sensory, physicochemical and nutritional properties were investigated. The dragee formulation was optimized and the characteristics of the optimal sample were compared with the control sample (containing dragee without SP). The results showed that adding SP increased the flavonoids, total anthocyanin content, vitamins, protein, minerals, essential and non-essential amino acids and fatty acids, including ω3 and ω6, while decreasing the energy intake. Based on the results, the optimal dragee sample was formulated and prepared with a desirability of 0.955. The correlation coefficient indicated that the effective optimization process and the performance of the model were carried out properly. The addition of SP had a significant impact on all color parameters considered by the panelists, and the enriched sample was given a very good taste score (75.10 ± 2.923) and an outstanding overall acceptance rate (91.20 ± 1.549) by the panelists. Although morphological data from scanning electron microscopy showed the distribution of non-uniform SP particles relative to the addition of SP in the extruded product formulation, the preservation of more nutritional properties and the good acceptance of sensory evaluators indicated the success of the application in dragee formulation. Therefore, instead of being utilized in an extruder, we discovered that SP may be used as a dragee for snack fortification.
Anti-Inflammatory Food in Asthma Prepared from Combination of Raphanus sativus L., Allium hookeri, Acanthopanax sessiliflorum, and Dendropanax morbiferus Extracts via Bioassay-Guided Selection
Authors: Lee Kyung-Dong , Shim Sun-Yup
Published: June 2022

Asthma is a highly prevalent inflammatory disease of the respiratory airways and an increasing health risk worldwide. Hence, finding new strategies to control or attenuate this condition is necessary. This study suggests nutraceuticals that are a combination of herbal plant extracts prepared from Acanthopanax sessiliflorum (AS), Codonopsis lanceolate (CL), Dendropanax morbiferus (DM), Allium hookeri (AH), and Raphanus sativus L. (RS) that can improve immunomodulatory ability through the detoxification and diuresis of air pollutants. Herbal parts (AH whole plant, RS and CL roots, AS and DM stems, and DM leaves) were selected, and four types of mixtures using plant extracts were prepared. Among these mixtures, M2 and M4 exhibited antioxidant activities in potent 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 1,1-diphenyl-β-picrylhydrazine (DPPH) radical assays. Moreover, M4 exhibited a marked increase in glutathione S-transferase (GST) activity and significantly inhibited the inflammatory mediator, nitric oxide (NO) and proinflammatory cytokines, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α generation. Furthermore, M4 exhibited the strongest antioxidant, hepatoprotective, and anti-inflammatory effects and was selected to prepare the product. Before manufacturing the product, we determined that the active mixture, M4, inhibited gene expression and generation of proinflammatory cytokines IL-1β, IL-6 and TNF-α in ovalbumin (OVA)-, lipopolysaccharide (LPS)-, and particulate matter (PM)-induced asthmatic rat models. The granular product (GP) was manufactured using M4 along with additives, i.e., lactose, oligosaccharide, stevioside extract, and nutmeg seed essential oils (flavor masking), in a ratio of 1:4 using a granulation machine, dried and ultimately packaged. The GP inhibited the generation of proinflammatory cytokines IL-1β, IL-6 and TNF-α in OVA-, LPS- and PM-induced asthmatic rat models. These results suggest that GP prepared from a combination of herbal plants (AS, CL, DM, AH and RS) is a potent functional food with anti-inflammatory activity that can be used to treat asthma caused by ambient air pollutants.
Anti-Spoilage Activity and Exopolysaccharides Production by Selected Lactic Acid Bacteria
Authors: Iosca Giovanna , De Vero Luciana , Di Rocco Giulia , Perrone Giancarlo , Gullo Maria , Pulvirenti Andrea
Published: June 2022

In this study, eight lactic acid bacteria (LAB) strains, previously isolated from traditional and gluten-free sourdoughs, and selected for their potential in improving the sensory and rheological quality of bakery products, were screened against some common spoilage agents. The anti-mould activity was tested using strains of the species Fusarium graminearum, Aspergillus flavus, Penicillium paneum and Aspergillus niger. Regarding the antibacterial activity, it was assessed against four strains of the species Escherichia coli, Campylobacter jejuni, Salmonella typhimurium and Listeria monocytogenes. Furthermore, LAB strains were evaluated for their ability to produce exopolysaccharides, which are gaining considerable attention for their functional properties and applicability in different food industrial applications. A strain-specific behaviour against the moulds was observed. In particular, F. graminearum ITEM 5356 was completely inhibited by all the LAB strains. Regarding the antibacterial activity, the strains Leuconostoc citreum UMCC 3011, Lactiplantibacillus plantarum UMCC 2996, and Pediococcus pentosaceus UMCC 3010 showed wide activity against the tested pathogens. Moreover, all the LAB strains were able to produce exopolysaccharides, which were preliminarily characterized. The assessed features of the LAB strains allow us to consider them as promising candidates for single or multiple starter cultures for food fermentation processes.
Adaptive Approaches for Tidal-Flow Lanes in Urban-Road Networks
Authors: Striewski Sören , Thomsen Ingo , Tomforde Sven
Published: June 2022

Every year, traffic congestion costs the global economy billions of dollars in lost productivity, particularly in urban areas. Traffic congestion is a complex problem, as traffic conditions may change at any time. Tidal-flow lanes can be utilised as a feasible traffic-congestion-mitigation strategy to balance the fluctuating traffic demands throughout the day. This paper proposes an adaptive-lane-reversal approach for tidal-flow lanes, to decrease the impact of traffic congestion in urban areas. In order to evaluate the adaptive approach under various traffic conditions, several algorithms and parameter sets are examined, using various network models and traffic demands. As a result, the total travel time of the vehicles in the various networks was decreased by up to 81%.
Anti-Fouling Performance of Hydrophobic Hydrogels with Unique Surface Hydrophobicity and Nanoarchitectonics
Authors: Zeng Liangpeng , Liu Ziqi , Huang Jingliang , Wang Xiaolin , Guo Hui , Li Wei-Hua
Published: June 2022

Hydrogel is a kind of soft and wet matter, which demonstrates favorable fouling resistance owing to the hydration anti-adhesive surfaces. Different from conventional hydrogels constructed by hydrophilic or amphiphilic polymers, the recently invented “hydrophobic hydrogels” composed of hydrophobic polymers exhibit many unique properties, e.g., surface hydrophobicity and high water content, suggesting promising applications in anti-fouling. In this paper, a series of hydrophobic hydrogels were prepared with different chemical structures and water content for anti-fouling investigations. The hydrophobic hydrogels showed high static water contact angles (WCAs > 90°), indicating remarkable surface hydrophobicity, which is abnormal for conventional hydrogels. Compared with the conventional hydrogels, all the hydrophobic hydrogels exhibited less than 4% E. coli biofilm coverage, showing a contrary trend of anti-fouling ability to the water content inside the polymer. Typically, the poly(2-(2-ethoxyethoxy)ethyl acrylate) (PCBA) and poly(tetrahydrofurfuryl acrylate) (PTHFA) hydrogels with relatively high surface hydrophobicity showed as low as 5.1% and 2.4% E. coli biofilm coverage even after incubation for 7 days in bacteria suspension, which are about 0.32 and 0.15 times of that on the hydrophilic poly(N,N-dimethylacrylamide) (PDMA) hydrogels, respectively. Moreover, the hydrophobic hydrogels exhibited a similar anti-adhesion ability and trend to algae S. platensis. Based on the results, the surface hydrophobicity mainly contributes to the excellent anti-fouling ability of hydrophobic hydrogels. In the meantime, the too-high water content may be somehow detrimental to anti-fouling performance.
Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
Authors: Zheng Juyun , Zhang Zeliang , Liang Yajun , Gong Zhaolong , Zhang Nala , Ditta Allah , Sang Zhiwei , Wang Junduo , Li Xueyuan
Published: June 2022

China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding procedures are therefore critical to the cotton industry’s success. The drought-resistant cotton variety Xinluzhong No. 82 and the drought-sensitive cotton variety Kexin No. 1 were utilised in this study to uncover a batch of drought-resistant candidate genes using whole transcriptome sequencing. The following are the key research findings: A competing endogenous RNA network (ceRNA) was built using complete transcriptional sequencing to screen the core genes in the core pathway, and two drought-related candidate genes were discovered. It was found that γ-aminobutyric acid aminotransferase (GhGABA-T, Gohir.A11G156000) was upregulated at 0 h vs. 12 h and downregulated at 12 h vs. 24 h. L-Aspartate oxidase (GhAO, Gohir.A07G220600) was downregulated at 0 h vs. 12 h and upregulated at 12 h vs. 24 h. GABA-T is analogous to a pyridoxal phosphate-dependent transferase superfamily protein (POP2) in Arabidopsis thaliana and influences plant drought resistance by controlling γ-aminobutyric acid (GABA) concentration. The analogue of GhAO in A. thaliana is involved in the early steps of nicotinamide adenine dinucleotide (NAD) production as well as in plant antioxidant responses. This study revealed that gene expression regulatory networks can be used for rapid screening of reliable drought resistance genes and then utilised to validate gene function.
Temperature Variation during Salt Migration in Frozen Hydrate-Bearing Sediments: Experimental Modeling
Authors: Chuvilin Evgeny , Ekimova Valentina , Davletshina Dinara , Bukhanov Boris , Krivokhat Ekaterina , Shilenkov Vladimir
Published: June 2022

Salt migration may be another reason why pore-gas hydrates dissociate in permafrost, besides pressure and temperature changes. Temperature variations in frozen hydrate-saturated sediments interacting with a NaCl solution have been studied experimentally at a constant temperature, ~−6 °C typical for permafrost. The experiments with frozen sandy samples containing metastable methane hydrate show that the migration of Na+ ions in the NaCl solution and their accumulation in the sediments can induce heat-consuming hydrate dissociation and ice melting. The hydrate-saturated frozen soils cool down at higher rates than their hydrate-free counterparts and require more time to recover their initial temperature. The temperature effects in hydrate-saturated frozen sediments exposed to contact with NaCl solutions depend strongly on salt concentration. The experimental results are used to model phase changes in the pore space associated with salt-ions transport and provide insights into the reasons for temperature changes.
Readiness of Mozambique Health Facilities to Address Undernutrition and Diarrhea in Children under Five: Indicators from 2018 and 2021 Survey Data
Authors: Sambo Júlia , Bauhofer Adilson Fernando Loforte , Boene Simone S. , Djedje Marlene , Júnior António , Pilale Adalgisa , Gonçalves Luzia , Deus Nilsa de , Chicumbe Sérgio
Published: June 2022

The World Health Organization’s systems framework shows that service delivery is key to addressing pressing health needs. Inadequate healthcare and the lack of healthcare services are factors associated with undernutrition and diarrhea in children under five, two health conditions with high morbi-mortality rates in Mozambique. The aim of the analysis was to determine the readiness score of nutrition and diarrhea services for children under five and the influence of malaria and HIV (Human Immunodeficiency Virus) service readiness on the readiness of these two services. A total of 1644 public health facilities in Mozambique were included from the 2018 Service Availability and Readiness Assessment. Additionally, a cross-sectional study was conducted to determine the availability and readiness scores of nutrition services in 2021 in five referral health facilities. The availability of nutrition and diarrhea services for children is low in Mozambique, with both scoring below 75%. Major unavailability was observed for human resources, guidelines, and training dimensions. Diarrhea (median (IQ): 72.2% (66.7 to 83.3)) and nutrition service readiness (median (IQ): 57.1% (52.4 to 57.1)) scores were significantly different (p < 0.001), while it is desirable for both services to be comprehensively ready. Nutrition services are positively associated with diarrhea service readiness and both services are associated with malaria and HIV service readiness (p < 0.05). None of the health facilities had all tracer items available and none of the facilities were considered ready (100%). There is a persisting need to invest comprehensively in readiness dimensions, within and across child health services.
The Effect of Augmented Reality-Based Proprioceptive Training Program on Balance, Positioning Sensation and Flexibility in Healthy Young Adults: A Randomized Controlled Trial
Authors: Lee Jaewon , Yu Jaeho , Hong Jiheon , Lee Dongyeop , Kim Jinseop , Kim Seonggil
Published: June 2022

This study investigates whether Augmented Reality (AR)-based interventions can be as effective as physical therapists (PT) regarding balance, positioning sensation, and flexibility. A sample of 39 regular people who voluntarily participated in this study were randomly distributed into two groups. Then AR was applied in the experimental group and PT was applied in the control group. Variables were measured by Tetrax (static balance), Y-balance test (dynamic balance), CSMI (proprioception), and sit and reach test (flexibility). All measurements were analyzed using paired t-test and independent t-test. The exercise program of this study improved the stability index (ST) of the static balance in both groups after the intervention, and there was a significant difference (p < 0.05) at normal eye close (NC) and Pillow with eye close (PC) positions. Moreover, regarding the case of dynamic balance, there were significant differences in AR and PT groups to reach in all directions (p < 0.05). In the case of positioning sensation, there was no significant difference in both groups (p > 0.05), and there was a significant difference in flexibility (p < 0.05). When comparing the two groups, there was no significant difference in all categories (p > 0.05). As a result, AR can be considered an effective form of therapy and can be selected according to individual conditions.
Anxiety Status of Female Chinese Ph.D. Candidates and Its Association with Sports
Authors: Mao Yupeng , Zhu Yongsheng , Jia Changjun , Sun Fengxin , Chen Song , Liu Bing
Published: June 2022

Given that stress leads to more anxiety among female Ph.D. candidates, more attention should be paid to their healthy lifestyle options. Several studies have shown that there is a negative correlation between sports and anxiety. This study took female Chinese Ph.D. candidates’ anxiety and sports participation as the research objects. A questionnaire entitled “Investigation on anxiety and sports of Ph.D. candidates” was used to explore the characteristics of anxiety in female Chinese Ph.D. candidates and to investigate the association between anxiety and sports in female Chinese Ph.D. candidates. A total of 588 Ph.D. candidates participated in the questionnaire survey. Some 21 invalid questionnaires were eliminated through the standard deviation of the items of the scale, and 567 valid questionnaires were finally obtained. The questionnaire survey was conducted online from 26 February to 15 March 2022, using the convenience sampling method. The results show that the anxiety level of female Chinese Ph.D. candidates is higher than that of male Ph.D. candidates and that the anxiety level of female Ph.D. candidates in a non-sports discipline is the highest. Weekly sports participation significantly lowers female Ph.D. candidates’ anxiety level (p < 0.01). Physical fatigue caused by study and work hinders female Ph.D. candidates from participating in sports (p < 0.05). Some female Chinese Ph.D. candidates in a negative emotional state are unwilling to participate in sports (p < 0.01). Future research should formulate different types of sports intervention programs suitable for alleviating the anxiety of female Ph.D. candidates.
The Crystallization Effect of Sodium Sulfate on Some Italian Marbles, Calcarenites and Sandstones
Authors: Lezzerini Marco , Tomei Alessio , Gallello Gianni , Aquino Andrea , Pagnotta Stefano
Published: June 2022

Soluble salts are compounds found inside ornamental rocks and building stones exposed to atmospheric agents in environments rich in alkaline metal ions, such as sodium and potassium. The damage induced by their crystallization in those materials, used to build monuments and architectural structures of great importance, is an unsolved problem. Sodium sulfate is one of the most common and harmful salt found in these constructions. In this work, we studied the resistance through time to the wet-drying cycles of some natural stones (calcarenites, marbles, and sandstones) that have been utilized in the historical architecture in Italy. Samples were freshly cut and thermally aged to simulate increasing decay. Induced porosity in the thermally degraded samples was high in calcarenites, medium in marbles, and low in sandstones. Specimens subjected to artificial thermal aging lost a major percentage of mass compared to the non-weathered ones, when affected by the crystallization of soluble salts. With this study, we have observed that samples subjected to different wetting and drying cycles degrade faster due to the action of soluble salts, compared to samples that are not subjected to these cycles.
Evaluation of Different Tomato (Solanum lycopersicum L.) Entries and Varieties for Performance and Adaptation in Mali, West Africa
Authors: Bihon Wubetu , Ognakossan Kukom Edoh , Tignegre Jean-Baptiste , Hanson Peter , Ndiaye Kabirou , Srinivasan Ramasamy
Published: June 2022

Tomato is an important vegetable crop and plays a major role in the food and nutrition security of the people of Mali. Production has increased in the recent decades but improvement in the fruit yield and quality remains suboptimal. Limited access to the best-adapted tomato varieties to the local conditions, pests and diseases are the major limiting factors for improving productivity. This study evaluated the performance of different tomato entries and varieties for their productivity, resistance to pests and diseases and postharvest fruit quality in Mali. Twenty-two entries and varieties of tomato in the rainy season and twenty-four in the dry season were evaluated. Varieties that were well adapted, better yielded, disease resistant and with good fruit quality were identified. Major plant diseases observed included tomato yellow leaf curve disease (TYLCD), bacterial wilt, bacterial leaf spot, early blight and southern blight. However, TYLCD was the major problem during the dry season. The variety of Icrixina was the most affected by TYLCD in both the rainy and dry seasons, although its total yield was not affected and remained one of the highest. Konica was one of the most susceptible varieties to bacterial wilt and bacterial leaf spot diseases. Tomato accession AVTO1710 provided the highest fruit yield (40.9 t/ha), while AVTO1704 provided the lowest (6.50 t/ha) in the rainy season. In contrast the highest yield during the dry growing season was 20 t/ha from VIO43614. Tomato entries and varieties varied in their postharvest fruit quality attributes (firmness, total soluble solid, pH and dry matter). Production season clearly influenced yield, disease occurrence and severity, as well as postharvest fruit qualities. The study identified better disease-resistant and yielding tomato entries suitable for rainy and dry growing seasons, which can be considered and scaled up for production so that farmers in Mali can produce tomato all year round.
Climatic Suitability of Different Areas in Abruzzo, Central Italy, for the Cultivation of Hazelnut
Authors: Di Lena Bruno , Curci Gabriele , Vergni Lorenzo , Farinelli Daniela
Published: June 2022

The demand for nuts has prompted the need to identify additional zones for hazelnut cultivation in Italy. There is great interest in the Abruzzo Region, in the central–eastern part of the country. The relationships between climate and environmental requirements for hazelnut were analyzed, taking into account the geography of the region, as well as climate change. The study was conducted by using the weather data from 34 stations between 1980 and 2019. The chilling requirements of the species are mostly met, except on the southern coast. Hot spring and summer caused an increase in the growing degree day in all locations. Years with minimum temperatures below −10 °C from December to March were almost null in coastal areas and the hilly belt. Late spring frosts in inland and mountainous areas occurred frequently, and the number of days with temperatures above 30 °C showed an upward trend everywhere. Five locations (Santo Stefano, Caramanico, Vasto, Isola del Gran Sasso, and Penne) were suitable for hazelnut cultivation, three were moderately suitable, seven were not very suitable, and nineteen were unsuitable.
Application of Machine Learning and Process-Based Models for Rainfall-Runoff Simulation in DuPage River Basin, Illinois
Authors: Bhusal Amrit , Parajuli Utsav , Regmi Sushmita , Kalra Ajay
Published: June 2022

Rainfall-runoff simulation is vital for planning and controlling flood control events. Hydrology modeling using Hydrological Engineering Center—Hydrologic Modeling System (HEC-HMS) is accepted globally for event-based or continuous simulation of the rainfall-runoff operation. Similarly, machine learning is a fast-growing discipline that offers numerous alternatives suitable for hydrology research’s high demands and limitations. Conventional and process-based models such as HEC-HMS are typically created at specific spatiotemporal scales and do not easily fit the diversified and complex input parameters. Therefore, in this research, the effectiveness of Random Forest, a machine learning model, was compared with HEC-HMS for the rainfall-runoff process. Furthermore, we also performed a hydraulic simulation in Hydrological Engineering Center—Geospatial River Analysis System (HEC-RAS) using the input discharge obtained from the Random Forest model. The reliability of the Random Forest model and the HEC-HMS model was evaluated using different statistical indexes. The coefficient of determination (R2), standard deviation ratio (RSR), and normalized root mean square error (NRMSE) were 0.94, 0.23, and 0.17 for the training data and 0.72, 0.56, and 0.26 for the testing data, respectively, for the Random Forest model. Similarly, the R2, RSR, and NRMSE were 0.99, 0.16, and 0.06 for the calibration period and 0.96, 0.35, and 0.10 for the validation period, respectively, for the HEC-HMS model. The Random Forest model slightly underestimated peak discharge values, whereas the HEC-HMS model slightly overestimated the peak discharge value. Statistical index values illustrated the good performance of the Random Forest and HEC-HMS models, which revealed the suitability of both models for hydrology analysis. In addition, the flood depth generated by HEC-RAS using the Random Forest predicted discharge underestimated the flood depth during the peak flooding event. This result proves that HEC-HMS could compensate Random Forest for the peak discharge and flood depth during extreme events. In conclusion, the integrated machine learning and physical-based model can provide more confidence in rainfall-runoff and flood depth prediction.
Factors Associated with Underutilization of Maternity Health Care Cascade in Mozambique: Analysis of the 2015 National Health Survey
Authors: Chicumbe Sérgio , Martins Maria do Rosário Oliveira
Published: June 2022

Maternity health care services utilization determines maternal and neonate outcomes. Evidence about factors associated with composite non-utilization of four or more antenatal consultations and intrapartum health care services is needed in Mozambique. This study uses data from the 2015 nationwide Mozambique’s Malaria, Immunization and HIV Indicators Survey. At selected representative households, women (n = 2629) with child aged up to 3 years answered a standardized structured questionnaire. Adjusted binary logistic regression assessed associations between women-child pairs characteristics and non-utilization of maternity health care. Seventy five percent (95% confidence interval (CI) = 71.8–77.7%) of women missed a health care cascade step during their last pregnancy. Higher education (adjusted odds ratio (AOR) = 0.65; 95% CI = 0.46–0.91), lowest wealth (AOR = 2.1; 95% CI = 1.2–3.7), rural residency (AOR = 1.5; 95% CI = 1.1–2.2), living distant from health facility (AOR = 1.5; 95% CI = 1.1–1.9) and unknown HIV status (AOR = 1.9; 95% CI = 1.4–2.7) were factors associated with non-utilization of the maternity health care cascade. The study highlights that, by 2015, recommended maternity health care cascade utilization did not cover 7 out of 10 pregnant women in Mozambique. Unfavorable sociodemographic and economic factors increase the relative odds for women not being covered by the maternity health care cascade.
Drivers of Inequalities among Families Involved with Child Welfare Services: A General Overview
Authors: Almquist Ylva B. , Straatmann Viviane S.
Published: June 2022

When parents are unable to raise their children or to meet the minimum acceptable standards for their care, this can lead to involvement with child welfare services [...]
Bibliometric Analysis of the Toxicity of Bisphenol A
Authors: Ni Mengmei , Li Xiaomeng , Zhang Lishi , Kumar Vikas , Chen Jinyao
Published: June 2022

Bisphenol A (BPA) is used worldwide and research on the toxicity of BPA has advanced rapidly in the last few decades. This study aimed to evaluate the global scientific output of toxicity of BPA and explore the hot spots and research trends. All available articles related to the toxicity of BPA until 2022 were retrieved from the Web of Science Core Collection database. The VOSviewer, a bibliometric analysis software, was used to analyze the information of included articles, including countries/institutions, international cooperation, journals, citations, and keywords. Among 1644 retrieved articles, 1611 eligible studies were identified for analysis, and the annual publications increased with time in the past three decades. China and the United States were the most active contributors in this field. Chinese Academy of Sciences and the Dow chemical company conducted relatively more research than others about BPA toxicity. The journal “Chemosphere” published the most studies on “BPA toxicity”. Before 2015, most research focused on estrogenic activity and the test system mainly utilized animal experiments. However, in recent years, research related to toxic mechanisms of BPA at the cellular level and the toxicity of its analogs have received widespread attention. Considering some critical research gaps, future research on BPA toxicology should probably focus on the molecular biology of toxic mechanism, mixture toxicity, and co-exposure of BPA substitutes. This study will help researchers understand past and current research trends, hot spots, and trends of toxicity studies of BPA and, thus, contribute to further research and risk management of BPA.
Relation between the Views and the Real Estate Application to a Mediterranean Coastal Area
Authors: Castro Noblejas Hugo , Vías Martínez Jesús , Mérida Rodríguez Matías F.
Published: June 2022

The landscape is a factor considered when choosing to purchase a dwelling, and, therefore, it influences the price of the real estate market. However, it is difficult to measure and assess its role, since it has a series of features that work in an integrated way and are hard to quantify separately. The characteristics of the views from each dwelling must also be considered, as well as their intrinsic characteristics or proximity to public services. This study proposes an automatic method to analyze the quality of the views, including both its dimensions and its composition in order to be able to estimate the economic weight of the views in the real estate value. A series of measurements of the views from 226 dwellings are integrated into the final index equation. The results are then compared with the estimated dwelling prices. The results highlight that the average price increases up to 18.1% in dwellings with a larger high-quality visual basin. It has also been noted that it is difficult to establish a correlation between the quality of the views and the housing prices due to the multifactorial nature of the housing prices.
Shear Stress Enhances the Paracrine-Mediated Immunoregulatory Function of Human Periodontal Ligament Stem Cells via the ERK Signalling Pathway
Authors: Suwittayarak Ravipha , Klincumhom Nuttha , Ngaokrajang Utapin , Namangkalakul Worachat , Ferreira João N. , Pavasant Prasit , Osathanon Thanaphum
Published: June 2022

Relevant immunomodulatory effects have been proposed following allogeneic cell-based therapy with human periodontal ligament stem cells (hPDLSCs). This study aimed to examine the influence of shear stress on the immunosuppressive capacity of hPDLSCs. Cells were subjected to shear stress at different magnitudes (0.5, 5 and 10 dyn/cm2). The expression of immunosuppressive markers was evaluated in shear stress-induced hPDLSCs using qRT-PCR, western blot, enzyme activity and enzyme-linked immunosorbent assays. The effects of a shear stress-derived condition medium (SS-CM) on T cell proliferation were examined using a resazurin assay. Treg differentiation was investigated using qRT-PCR and flow cytometry analysis. Our results revealed that shear stress increased mRNA expression of IDO and COX2 but not TGF-β1 and IFN-γ. IDO activity, kynurenine and active TGF-β1 increased in SS-CM when compared to the non-shear stress-derived conditioned medium (CTL-CM). The amount of kynurenine in SS-CM was reduced in the presence of cycloheximide and ERK inhibitor. Subsequently, T cell proliferation decreased in SS-CM compared to CTL-CM. Treg differentiation was promoted in SS-CM, indicated by FOXP3, IL-10 expression and CD4+CD25hiCD127lo/− subpopulation. In conclusion, shear stress promotes kynurenine production through ERK signalling in hPDLSC, leading to the inhibition of T cell proliferation and the promotion of Treg cell differentiation.
Changes in the Expression of Smooth Muscle Cell–Related Genes in Human Dermal Sheath Cup Cells Associated with the Treatment Outcome of Autologous Cell–Based Therapy for Male and Female Pattern Hair Loss
Authors: Yoshida Yuzo , Takahashi Miki , Yamanishi Haruyo , Nakazawa Yosuke , Kishimoto Jiro , Ohyama Manabu
Published: June 2022

In a clinical study of autologous cell–based therapy using dermal sheath cup (DSC) cells, the treatment of hair loss showed improvements. However, the outcomes were variable. Here, correlations between marker gene expression in DSC cells and treatment outcomes were assessed to predict therapeutic efficacy. Overall, 32 DSC cell lines were used to evaluate correlations between marker gene expression and treatment outcomes. Correlations between vascular pericyte and preadipocyte marker expression and treatment outcomes were inconsistent. As smooth muscle cell markers, MYOCD correlated negatively with treatment outcomes and SRF consistently demonstrated an inverse correlation. Additionally, CALD1 correlated negatively and ACTA2 correlated inversely with treatment outcomes. DSC cell lines were divided into good and moderate/poor responders to further investigate the correlations. SRF and CALD1 were lower in a good responder compared with a moderate responder. Next, DSC cells were differentiated toward dermal papilla cells. Dermal papilla markers SOX2 and LEF1 before differentiation had moderate positive and inverse correlations with the treatment outcome, respectively. SOX2 after differentiation more consistently demonstrated a positive correlation. Significant downregulation of smooth muscle–related genes was also observed after differentiation. These findings revealed putative markers for preclinical evaluation of DSC cells to improve hair loss.
Outcome Prediction in Critically-Ill Patients with Venous Thromboembolism and/or Cancer Using Machine Learning Algorithms: External Validation and Comparison with Scoring Systems
Authors: Danilatou Vasiliki , Nikolakakis Stylianos , Antonakaki Despoina , Tzagkarakis Christos , Mavroidis Dimitrios , Kostoulas Theodoros , Ioannidis Sotirios
Published: June 2022

Intensive care unit (ICU) patients with venous thromboembolism (VTE) and/or cancer suffer from high mortality rates. Mortality prediction in the ICU has been a major medical challenge for which several scoring systems exist but lack in specificity. This study focuses on two target groups, namely patients with thrombosis or cancer. The main goal is to develop and validate interpretable machine learning (ML) models to predict early and late mortality, while exploiting all available data stored in the medical record. To this end, retrospective data from two freely accessible databases, MIMIC-III and eICU, were used. Well-established ML algorithms were implemented utilizing automated and purposely built ML frameworks for addressing class imbalance. Prediction of early mortality showed excellent performance in both disease categories, in terms of the area under the receiver operating characteristic curve (AUC–ROC): VTE-MIMIC-III 0.93, eICU 0.87, cancer-MIMIC-III 0.94. On the other hand, late mortality prediction showed lower performance, i.e., AUC–ROC: VTE 0.82, cancer 0.74–0.88. The predictive model of early mortality developed from 1651 VTE patients (MIMIC-III) ended up with a signature of 35 features and was externally validated in 2659 patients from the eICU dataset. Our model outperformed traditional scoring systems in predicting early as well as late mortality. Novel biomarkers, such as red cell distribution width, were identified.
Glycation of Tie-2 Inhibits Angiopoietin-1 Signaling Activation and Angiopoietin-1-Induced Angiogenesis
Authors: Zhou Haiyan , Chen Tangting , Li Yongjie , You Jingcan , Deng Xin , Chen Ni , Li Tian , Zheng Youkun , Li Rong , Luo Mao , Wu Jianbo , Wang Liqun
Published: June 2022

The impairment of the angiopoietin-1 (Ang-1)/Tie-2 signaling pathway has been thought to play a critical role in diabetic complications. However, the underlying mechanisms remain unclear. The present study aims to investigate the effects of Tie-2 glycation on Ang-1 signaling activation and Ang-1-induced angiogenesis. We identified that Tie-2 was modified by advanced glycation end products (AGEs) in aortae derived from high fat diet (HFD)-fed mice and in methylglyoxal (MGO)-treated human umbilical vein endothelial cells (HUVECs). MGO-induced Tie-2 glycation significantly inhibited Ang-1-evoked Tie-2 and Akt phosphorylation and Ang-1-regulated endothelial cell migration and tube formation, whereas the blockade of AGE formation by aminoguanidine remarkably rescued Ang-1 signaling activation and Ang-1-induced angiogenesis in vitro. Furthermore, MGO treatment markedly increased AGE cross-linking of Tie-2 in cultured aortae ex vivo and MGO-induced Tie-2 glycation also significantly decreased Ang-1-induced vessel outgrow from aortic rings. Collectively, these data suggest that Tie-2 may be modified by AGEs in diabetes mellitus and that Tie-2 glycation inhibits Ang-1 signaling activation and Ang-1-induced angiogenesis. This may provide a novel mechanism for Ang-1/Tie-2 signal dysfunction and angiogenesis failure in diabetic ischaemic diseases.