bluuebunny/crossref_metadata_2025 huggingface dataset:
Operator-Based Nonlinear Control for a Miniature Flexible Actuator Using the Funnel Control Method
Authors: Ueno Keisuke , Kawamura Shuhei , Deng Mingcong
Published: February 2021

Recently, the studies of soft actuators have been getting increased attention among various fields. Soft actuators are very safe for fragile objects and have an affinity to humans because they are composed of flexible materials. A miniature flexible actuator is a kind of pneumatically driven soft actuator. It has a bellowed shape and asymmetrical structure. This shape can generate a curling motion in two ways under positive and negative pressures with only one air tube. In the previous article, a control system using adaptive λ-tracking control was proposed. This control gain can become too large as time tends to infinity because the adaptive law exhibits a non-decreasing gain. To solve this problem, the funnel control method is proposed. The adaptive gain of this method not only increases but also decreases; however, the design scheme of the boundary function which is needed to decide on adaptive gain is not proposed here. In this article, an operator-based nonlinear control system’s design and the design scheme of the boundary function using an observer are proposed. Then, the effectiveness of the proposed method is verified by a simulation and an experiment.
Magnetic Field Inhibition of Convective Heat Transfer in Magnetic Nanofluid
Authors: Zakinyan Arthur , Kunikin Stanislav , Chernyshov Andrey , Aitov Vitali
Published: February 2021

Natural convection is the main mechanism of heat transfer in many natural and technological processes, which makes it urgent to study the possibilities of controlling it. In this work, the processes of development and damping of thermal convection in a flat vertical quasi-two-dimensional layer of magnetic nanofluid are considered experimentally. The presence of the magnetic properties of the nanofluid makes it possible to effectively apply the external magnetic fields to regulate convective heat transfer. The magnetic nanofluid layer was heated from below. It was shown in this work that the imposition of an external uniform stationary magnetic field perpendicular to the temperature gradient leads to the suppression of convection. The processes of heating and cooling the metal plates in a magnetic nanofluid are studied. It is demonstrated that the suppression of convection by a magnetic field leads to a slowdown in the heating of cold and cooling of hot metal plates in a magnetic nanofluid. The obtained results can be considered as a model for understanding similar exchange processes in liquids under the action of magnetic field.
A Robust Share-of-Choice Model
Authors: Moresino Francesco
Published: February 2021

In this paper, we propose an approach to take into account, in a robust way, part-worth uncertainty in a share-of-choice (SOC) model. More precisely, we extend the method proposed by Wang and Curry by endogenously including competition. Indeed in their approach, competition is described exogenously and the model cannot take into account part-worth uncertainty for the competition’s products. Our extension permits us to take into account all effects of part-worth uncertainty, even those relative to the competition, and therefore improve substantially Wang and Curry’s approach.
Discrete Group Actions on Digital Objects and Fixed Point Sets by Isok(·)-Actions
Authors: Han Sang-Eon
Published: February 2021

Given a digital image (or digital object) (X,k),X⊂Zn, this paper initially establishes a group structure of the set of self-k-isomorphisms of (X,k) with the function composition, denoted by Isok(X) or Autk(X). In particular, let Ckn,l be a simple closed k-curve with l elements in Zn. Then, the group Isok(Ckn,l) is proved to be isomorphic to the standard dihedral group Dl with order l. The calculation of this quantity Isok(Ckn,l) is a key step for obtaining many new results. Indeed, it is essential for exploring many features of Isok(X). Furthermore, this quantity is proved to be a digital topological invariant. After proceeding with an Isok(X)-action on (X,k), we investigate some properties of fixed point sets by this action. Finally, we explore various features of fixed point sets by this action from the viewpoint of digital k-curve theory. This paper only deals with k-connected digital images (X,k) whose cardinality is equal to or greater than 2. Besides, we discuss some errors that have appeared in the lilterature.
A Multi-Strategy Marine Predator Algorithm and Its Application in Joint Regularization Semi-Supervised ELM
Authors: Yang Wenbiao , Xia Kewen , Li Tiejun , Xie Min , Song Fei
Published: February 2021

A novel semi-supervised learning method is proposed to better utilize labeled and unlabeled samples to improve classification performance. However, there is exists the limitation that Laplace regularization in a semi-supervised extreme learning machine (SSELM) tends to lead to poor generalization ability and it ignores the role of labeled information. To solve the above problems, a Joint Regularized Semi-Supervised Extreme Learning Machine (JRSSELM) is proposed, which uses Hessian regularization instead of Laplace regularization and adds supervised information regularization. In order to solve the problem of slow convergence speed and the easy to fall into local optimum of marine predator algorithm (MPA), a multi-strategy marine predator algorithm (MSMPA) is proposed, which first uses a chaotic opposition learning strategy to generate high-quality initial population, then uses adaptive inertia weights and adaptive step control factor to improve the exploration, utilization, and convergence speed, and then uses neighborhood dimensional learning strategy to maintain population diversity. The parameters in JRSSELM are then optimized using MSMPA. The MSMPA-JRSSELM is applied to logging oil formation identification. The experimental results show that MSMPA shows obvious superiority and strong competitiveness in terms of convergence accuracy and convergence speed. Also, the classification performance of MSMPA-JRSSELM is better than other classification methods, and the practical application is remarkable.
Marine Heterocyclic Compounds That Modulate Intracellular Calcium Signals: Chemistry and Synthesis Approaches
Authors: González-Andrés Paula , Fernández-Peña Laura , Díez-Poza Carlos , Villalobos Carlos , Nuñez Lucía , Barbero Asunción
Published: February 2021

Intracellular Ca2+ plays a pivotal role in the control of a large series of cell functions in all types of cells, from neurotransmitter release and muscle contraction to gene expression, cell proliferation and cell death. Ca2+ is transported through specific channels and transporters in the plasma membrane and subcellular organelles such as the endoplasmic reticulum and mitochondria. Therefore, dysregulation of intracellular Ca2+ homeostasis may lead to cell dysfunction and disease. Accordingly, chemical compounds from natural origin and/or synthesis targeting directly or indirectly these channels and proteins may be of interest for the treatment of cell dysfunction and disease. In this review, we show an overview of a group of marine drugs that, from the structural point of view, contain one or various heterocyclic units in their core structure, and from the biological side, they have a direct influence on the transport of calcium in the cell. The marine compounds covered in this review are divided into three groups, which correspond with their direct biological activity, such as compounds with a direct influence in the calcium channel, compounds with a direct effect on the cytoskeleton and drugs with an effect on cancer cell proliferation. For each target, we describe its bioactive properties and synthetic approaches. The wide variety of chemical structures compiled in this review and their significant medical properties may attract the attention of many different researchers.
Antiproliferative Activity and Potential Mechanism of Marine-Sourced Streptoglutarimide H against Lung Cancer Cells
Authors: Ge Hengju , Zhang Di , Shi Muran , Lian Xiaoyuan , Zhang Zhizhen
Published: February 2021

In 2019, streptoglutarimide H (SGH) was characterized as a new glutarimide from the secondary metabolites produced by a marine-derived actinomycete Streptomyces sp. ZZ741 and shown to have in vitro antiglioma activity. However, the antiproliferative activity and potential mechanism of SGH against lung cancer cells have not yet been characterized. This study demonstrated that SGH significantly inhibited the proliferation of different lung cancer cells. In terms of mechanism of action, SGH downregulated cell cycle- and nucleotide synthesis-related proteins to block cell cycle at G0/G1 phase, reduced the expression levels of glycolytic metabolic enzymes to inhibit glycolysis, and downregulated the important cancer transcription factor c-Myc and the therapeutic target deubiquitinase USP28. Potent anticancer activity and multiple mechanisms indicated SGH to be a novel antitumor compound against lung cancer cells.
Preventive Effect of Depolymerized Sulfated Galactans from Eucheuma serra on Enterotoxigenic Escherichia coli-Caused Diarrhea via Modulating Intestinal Flora in Mice
Authors: Ma Yu , Zhang Qian , Liu Wenqiang , Chen Zhaohua , Zou Chao , Fu Linglin , Wang Yanbo , Liu Yixiang
Published: February 2021

In this work, the preventive effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on bacterial diarrhea by regulating intestinal flora was investigated in vivo. Based on the enterotoxigenic Escherichia coli (ETEC)-infected mouse diarrhea model, DESP at doses ranging from 50 mg/kg to 200 mg/kg alleviated weight loss and decreased the diarrhea rate and diarrhea index. Serological tests showed that the levels of inflammation-related factors were effectively suppressed. Furthermore, the repaired intestinal mucosa was verified by morphology and pathological tissue section observations. Compared with the model group, the richness and diversity of the intestinal flora in the DESP group increased according to the 16S rRNA high-throughput sequencing of the gut microbiota. Specifically, Firmicutes and Actinobacteria increased, and Proteobacteria decreased after DESP administration. At the family level, DESP effectively improved the abundance of Lactobacillaceae, Bifidobacteriaceae, and Lachnospiraceae, while significantly inhibiting the growth of Enterobacteriaceae. Therefore, the antimicrobial diarrhea function of DESP may be related to the regulation of intestinal microbiota.
Premature Mortality Due to Chronic Obstructive Pulmonary Disease (COPD) in Poland
Authors: Moryson Waclaw , Stawinska-Witoszynska Barbara
Published: February 2021

Background and objectives: Chronic obstructive pulmonary disease (COPD) is responsible for 5.3% of deaths worldwide and constitutes the third most common cause of death. The deaths from this cause occur over 10times more often in smokers than in non-smokers. Fortunately, for nearly 30 years, the proportion of people smoking tobacco in Poland has been decreasing. This study aims to analyse the change in premature mortality rates of men and women due to COPD in Poland during 2008–2017. Materials and Methods: The time trends of deaths occurring under 65 were analysed. Standardised premature mortality rates were used, as well as the mortality rates for the five-year age ranges, with a breakdown by gender. The Joinpoint model was used to determine time trends. Results: Over the period analysed, premature mortality in the female population decreased by 2.6% from year to year, albeit without statistical significance, and in the male population there was a decrease by statistically significant 5.2% per year. The biggest drop in mortality, almost 10% per year, was observed in the group of females aged between 50 and 54. Among males, the most significant reduction in mortality was observed in groups between the ages of 40 and 54, and it amounted to approximately 8% annually. With increasing age, the dynamics of mortality reduction decreased. Conclusions: The study showed a steady downward trend in premature mortality due to chronic obstructive pulmonary disease in Poland in both genders. The reduction in mortality was at a high level, despite the lower mortality due to this cause than in other European countries.
Raised Source/Drain (RSD) and Vertical Lightly Doped Drain (LDD) Poly-Si Thin-Film Transistor
Authors: Chien Feng-Tso , Ye Jing , Yen Wei-Cheng , Chen Chii-Wen , Lin Cheng-Li , Tsai Yao-Tsung
Published: February 2021

The raised source/drain (RSD) structure is one of thin film transistor designs that is often used to improve device characteristics. Many studies have mentioned that the high impact ionization rate occurring at a drain side can be reduced, owing to a raised source/drain area that can disperse the drain electric field. In this study, we will discuss how the electric field at the drain side of an RSD device is reduced by a vertical lightly doped drain (LDD) scheme rather than a RSD structure. We used different raised source/drain forms to simulate the drain side electric field for each device, as well as their output characteristics, using Integrated Systems Engineering (ISE-TCAD) simulators. Different source and drain thicknesses and doping profiles were applied to verify the RSD mechanism. We found that the electric fields of a traditional device and uniform doping RSD structures are almost the same (~2.9 × 105 V/cm). The maximum drain electric field could be reduced to ~2 × 105 V/cm if a vertical lightly doped drain RSD scheme was adopted. A pure raised source/drain structure did not benefit the device characteristics if a vertical lightly doped drain design was not included in the raised source/drain areas.
Structure and Properties of Ca and Zr Containing Heat Resistant Wire Aluminum Alloy Manufactured by Electromagnetic Casting
Authors: Belov Nikolay , Akopyan Torgom , Korotkova Natalia , Murashkin Maxim , Timofeev Victor , Fortuna Anastasiya
Published: February 2021

Experimental aluminum alloy containing 0.8% Ca, 0.5% Zr, 0.5% Fe and 0.25% Si (wt.%), in the form of a long-length rod 12 mm in diameter was manufactured using an electromagnetic casting (EMC) technique. The extremely high cooling rate during alloy solidification (≈104 K/s) caused the formation of a favorable microstructure in the ingot characterized by a small size of the dendritic cells, fine eutectic particles of Ca-containing phases and full dissolution of Zr in Al the solid solution. Due to the microstructure obtained the ingots possess high manufacturability during cold forming (both drawing and rolling). Analysis of the electrical conductivity (EC) and microhardness of the cold rolled strip and cold drawn wire revealed that their temperature dependences are very close. The best combination of hardness and EC in the cold rolled strip was reached after annealing at 450 °C. TEM study of structure evolution revealed that the annealing mode used leads to the formation of L12 type Al3Zr phase precipitates with an average diameter of 10 nm and a high number density. Experimental wire alloy has the best combination of ultimate tensile strength (UTS), electrical conductivity (EC) (200 MPa and 54.8% IACS, respectively) and thermal stability (up to 450 °C) as compared with alloys based on the Al–Zr and Al– rare-earth metal (REM) systems. In addition, it is shown that the presence of calcium in the model alloy increases the electrical conductivity after cold forming operations (both drawing and rolling).
The Effects of SGLT2 Inhibitors on Lipid Metabolism
Authors: Szekeres Zsolt , Toth Kalman , Szabados Eszter
Published: February 2021

Sodium glucose co-transporter 2 (SGLT2) inhibitors are effective antihyperglycemic agents by inhibiting glucose reabsorption in the proximal tubule of the kidney. Besides improving glycemic control in patients with type 2 diabetes, they also have additional favorable effects, such as lowering body weight and body fat. Several clinical studies have demonstrated their positive effect in reducing cardiovascular morbidity and mortality. Furthermore, the use of SGLT2 inhibitors were associated with fewer adverse renal outcomes comparing to other diabetic agents, substantiating their renoprotective effect in diabetic patients. SGLT2 inhibitors have also remarkable effect on lipid metabolism acting at different cellular levels. By decreasing the lipid accumulation, visceral and subcutaneous fat, they do not only decrease the body weight but also change body composition. They also regulate key molecules in lipid synthesis and transportation, and they affect the oxidation of fatty acids. Notably, they shift substrate utilization from carbohydrates to lipids and ketone bodies. In this review we intended to summarize the role of SGLT2 inhibitors in lipid metabolism especially on lipoprotein levels, lipid regulation, fat storage and substrate utilization.
Crossing the Wall: Characterization of the Multiheme Cytochromes Involved in the Extracellular Electron Transfer Pathway of Thermincola ferriacetica
Authors: Faustino Marisa M. , Fonseca Bruno M. , Costa Nazua L. , Lousa Diana , Louro Ricardo O. , Paquete Catarina M.
Published: February 2021

Bioelectrochemical systems (BES) are emerging as a suite of versatile sustainable technologies to produce electricity and added-value compounds from renewable and carbon-neutral sources using electroactive organisms. The incomplete knowledge on the molecular processes that allow electroactive organisms to exchange electrons with electrodes has prevented their real-world implementation. In this manuscript we investigate the extracellular electron transfer processes performed by the thermophilic Gram-positive bacteria belonging to the Thermincola genus, which were found to produce higher levels of current and tolerate higher temperatures in BES than mesophilic Gram-negative bacteria. In our study, three multiheme c-type cytochromes, Tfer_0070, Tfer_0075, and Tfer_1887, proposed to be involved in the extracellular electron transfer pathway of T. ferriacetica, were cloned and over-expressed in E. coli. Tfer_0070 (ImdcA) and Tfer_1887 (PdcA) were purified and biochemically characterized. The electrochemical characterization of these proteins supports a pathway of extracellular electron transfer via these two proteins. By contrast, Tfer_0075 (CwcA) could not be stabilized in solution, in agreement with its proposed insertion in the peptidoglycan wall. However, based on the homology with the outer-membrane cytochrome OmcS, a structural model for CwcA was developed, providing a molecular perspective into the mechanisms of electron transfer across the peptidoglycan layer in Thermincola.
Potential Association between Vaginal Microbiota and Cervical Carcinogenesis in Korean Women: A Cohort Study
Authors: Kang Gi-Ung , Jung Da-Ryung , Lee Yoon Hee , Jeon Se Young , Han Hyung Soo , Chong Gun Oh , Shin Jae-Ho
Published: February 2021

Convincing studies demonstrated that vaginal flora is one of the most impactful key components for the well-being of the genital tract in women. Nevertheless, the potential capability of vaginal-derived bacterial communities as biomarkers to monitor cervical carcinogenesis (CC) has yet to be studied actively compared to those of bacterial vaginosis (BV). We hypothesized that vaginal microbiota might be associated with the progression of CC. In this study, we enrolled 23 participants, including healthy controls (HC group; n = 7), patients with cervical intraepithelial neoplasia (CIN) 2 and 3 (CIN group, n = 8), and patients with invasive cervical cancer (CAN group; n = 8). Amplicon sequencing was performed using the Ion Torrent PGM to characterize the vaginal microbiota. Patients with CIN and CAN presented vaginal microbiota dysbiosis compared with HC. The alpha diversity analysis revealed that CC has a trend to be increased in terms of diversity indexes. Moreover, CC was associated with the abundance of specific microbes, of which Lactobacillus and Gardnerella were the most significantly different between HC and CIN, whereas Streptococcus was differentially abundant in CAN compared with CIN. We then evaluated their diagnostic abilities. Testing in terms of diagnostic ability using the three genera revealed considerably high performance with an area under the receiver-operating characteristic curve of 0.982, 0.953, and 0.922. The current study suggests that the presence of Gardnerella and Streptococcus may be involved in the advancment of CC.
Systematic Review of Machine Learning Applications in Mining: Exploration, Exploitation, and Reclamation
Authors: Jung Dahee , Choi Yosoon
Published: February 2021

Recent developments in smart mining technology have enabled the production, collection, and sharing of a large amount of data in real time. Therefore, research employing machine learning (ML) that utilizes these data is being actively conducted in the mining industry. In this study, we reviewed 109 research papers, published over the past decade, that discuss ML techniques for mineral exploration, exploitation, and mine reclamation. Research trends, ML models, and evaluation methods primarily discussed in the 109 papers were systematically analyzed. The results demonstrated that ML studies have been actively conducted in the mining industry since 2018, mostly for mineral exploration. Among the ML models, support vector machine was utilized the most, followed by deep learning models. The ML models were evaluated mostly in terms of their root mean square error and coefficient of determination.
Mineralogical Characterization of Carreaux de Pavement from Northern Spain (Tiebas, Navarre)
Authors: Ruiz-Ardanaz Iván , Lasheras Esther , Durán Adrián
Published: February 2021

Several medieval tiles from Tiebas Castle in Navarre, classified as carreaux de pavement, were mineralogically analyzed. The aim was to add information to the very scarce analytical data available for carreaux de pavement in order to provide a quality primary work, mainly descriptive, in this topic. The characterization techniques applied were: optical microscopy (OM), colorimetry, Fourier transform infrared (FT-IR) spectroscopy, differential thermal analysis and thermogravimetry (DTA-TG), X-ray fluorescence (XRF) spectroscopy, petrographic microscopy (PM), X-ray diffraction (XRD), scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDS). The tiles comprised three layers: a top glaze with mainly silicon and lead-based compounds; a thin layer of silicoaluminate (very possibly kaolinite) called engobe under it; and the pastes, composed of quartz, hematite, potassium feldspars and calcite. Honey glazes were richer in iron, being copper-based compounds responsible for dark green glazes. The orangish-reddish color of the pastes was provided by hematite. Although the tiles were found in three different locations within the castle, no significant differences were detected among them. The carreaux de pavement from Tiebas had similarities with that from the Bordeaux region. According to the composition data and thickness of tiles from other French carreaux de pavement, the Tiebas artifacts were dated to the 13th century.
Effect of Talc in Mixtures with Fly Ash on Sintering Crystalline Phases and Porosity of Mullite-Cordierite Ceramics
Authors: Valášková Marta , Blahůšková Veronika , Martaus Alexandr , Študentová Soňa , Vallová Silvie , Tokarský Jonáš
Published: February 2021

The effect of talc in the two mixtures with the representative sample of fly ash (Class F) was investigated at sintering temperatures of 1000, 1100, and 1200 °C. X-ray diffraction, thermal DTA/TGA, and mercury intrusion porosimetry analyses were applied to characterize the mineral phase transformation of talc and fly ash in cordierite ceramic. The influence of iron oxide on talc transformation to Fe-enstatite was verified by the simulated molecular models and calculated XRD patterns and the assumption of Fe-cordierite crystallization was confirmed. The fly ash mixtures with 10 mass% of talc in comparison with 30 mass% of talc at 1000 °C and 1100 °C showed higher linear shrinkage and lower porosity. At a temperature of 1200 °C, sintering expansion and larger pores in mullite and cordierite ceramics also containing sapphirine and osumilite demonstrated that magnesium in FA and Tc structure did not react with the other constituents to form crystalline cordierite. The ceramics produced in the present work using fly ash and talc have similar properties to the commercial ceramics produced at sintering temperatures higher than 1250 °C.
Nodule Count, End of Solidification Cooling Rate, and Shrinkage Porosity Correlations in High Silicon Spheroidal Graphite Iron
Authors: Alonso Gorka , Stefanescu Doru Michael , Bravo Beñat , Zarrabeitia Gorka , Suarez Ramon
Published: February 2021

High-silicon spheroidal graphite (SG) irons present higher changes of density during the solidification process when compared to normal SG irons. This special behavior is particularly significant in the last stages of solidification, where the graphite expansion may become insufficient to compensate the contraction of the austenite and the risk of microporosity formation increases. The goal of this laboratory research was to establish correlations between the different levels of nodule count obtained using five commercial inoculants, the cooling rate at the end of solidification, and the shrinkage porosity propensity. The analysis was conducted on thermal analysis cups that were sectioned and evaluated for microstructure by optical metallography and by 2D analysis with the Image J software to quantify the size of the microporosity region. It was found that a higher nodule count, associated with higher cooling rate at the end of solidification, generates lower porosity. SEM analysis was conducted to study the nature of nuclei. Complex (MgSiAl)N nitrides were found as the main nucleation sites for graphite.
Meso/Microporous Carbons from Conjugated Hyper-Crosslinked Polymers Based on Tetraphenylethene for High-Performance CO2 Capture and Supercapacitor
Authors: Mohamed Mohamed Gamal , Ahmed Mahmoud M. M. , Du Wei-Ting , Kuo Shiao-Wei
Published: February 2021

In this study, we successfully synthesized two types of meso/microporous carbon materials through the carbonization and potassium hydroxide (KOH) activation for two different kinds of hyper-crosslinked polymers of TPE-CPOP1 and TPE-CPOP2, which were synthesized by using Friedel–Crafts reaction of tetraphenylethene (TPE) monomer with or without cyanuric chloride in the presence of AlCl3 as a catalyst. The resultant porous carbon materials exhibited the high specific area (up to 1100 m2 g−1), total pore volume, good thermal stability, and amorphous character based on thermogravimetric (TGA), N2 adsoprtion/desorption, and powder X-ray diffraction (PXRD) analyses. The as-prepared TPE-CPOP1 after thermal treatment at 800 °C (TPE-CPOP1-800) displayed excellent CO2 uptake performance (1.74 mmol g−1 at 298 K and 3.19 mmol g−1 at 273 K). Furthermore, this material possesses a high specific capacitance of 453 F g−1 at 5 mV s−1 comparable to others porous carbon materials with excellent columbic efficiencies for 10,000 cycle at 20 A g−1.
A New Benchmark of Charges Storage in Single-Layer Organic Light-Emitting Diodes Based on Electrical and Optical Characteristics
Authors: Zhang Chengwen , Xu Zheng , Wang Peng , Qin Zilun , Wageh S. , Al-Ghamdi Ahmed , Zhao Suling
Published: February 2021

The storage of charges in organic light-emitting diodes (OLEDs) has drawn much attention for its damage to device performance as well as the loss to carriers. Thus, it is essential to address the issue and do further investigation. The traditional approach to storage analysis is mainly based on transient measurement since it is sensitive to transient instead of steady signal. In this paper, we proposed a new benchmark to investigate the single-layer OLEDs capable of stored charges with poly (methyl methacrylate) (PMMA), which is just based on electrical and optical characteristics. Since the stored charges contribute both to luminance and current of the devices with PMMA, the area between them can be taken as a benchmark and evaluated the storage of charges. In our experiment, the areas of 4 nm, 6 nm, 8 nm, and 10 nm PMMA devices are 0.348, 0.554, 0.808, and 0.894, respectively, indicating a higher capability of storage in thicker PMMA. It is exactly in line with the results taken from transient electroluminescence (EL) measurement. Thus, this new benchmark is practical and provides a more accessible approach to investigate the storage of charges in OLEDs.
Bioactive Secoiridoids in Italian Extra-Virgin Olive Oils: Impact of Olive Plant Cultivars, Cultivation Regions and Processing
Authors: Losito Ilario , Abbattista Ramona , De Ceglie Cristina , Castellaneta Andrea , Calvano Cosima Damiana , Cataldi Tommaso R.I.
Published: February 2021

In the last two decades, phenolic compounds occurring in olive oils known as secoiridoids have attracted a great interest for their bioactivity. Four major olive oil secoiridoids, i.e., oleuropein and ligstroside aglycones, oleacin and oleocanthal, were previously characterized in our laboratory using reversed-phase liquid chromatography with electrospray ionization-Fourier transform-mass spectrometry (RPLC-ESI-FTMS). The same analytical approach, followed by multivariate statistical analysis (i.e., Principal Component Analysis), was applied here to a set of 60 Italian extra-virgin olive oils (EVOO). The aim was to assess the secoiridoid contents as a function of olive cultivars, place of cultivation (i.e., different Italian regions) and olive oil processing, in particular two- vs. three-phase horizontal centrifugation. As expected, higher secoiridoid contents were generally found in olive oils produced by two-phase horizontal centrifugation. Moreover, some region/cultivar-related trends were evidenced, as oleuropein and ligstroside aglycones prevailed in olive oils produced in Apulia (Southern Italy), whereas the contents of oleacin and oleocanthal were relatively higher in EVOO produced in Central Italy (Tuscany, Lazio and Umbria). A lower content of all the four secoiridoids was generally found in EVOO produced in Sicily (Southern Italy) due to the intrinsic low abundance of these bioactive compounds in cultivars typical of that region.
Bioethanol Production by Enzymatic Hydrolysis from Different Lignocellulosic Sources
Authors: Vasić Katja , Knez Željko , Leitgeb Maja
Published: February 2021

As the need for non-renewable sources such as fossil fuels has increased during the last few decades, the search for sustainable and renewable alternative sources has gained growing interest. Enzymatic hydrolysis in bioethanol production presents an important step, where sugars that are fermented are obtained in the final fermentation process. In the process of enzymatic hydrolysis, more and more new effective enzymes are being researched to ensure a more cost-effective process. There are many different enzyme strategies implemented in hydrolysis protocols, where different lignocellulosic biomass, such as wood feedstocks, different agricultural wastes, and marine algae are being used as substrates for an efficient bioethanol production. This review investigates the very recent enzymatic hydrolysis pathways in bioethanol production from lignocellulosic biomass.
Granular Hall Sensors for Scanning Probe Microscopy
Authors: Sachser Roland , Hütner Johanna , Schwalb Christian H. , Huth Michael
Published: February 2021

Scanning Hall probe microscopy is attractive for minimally invasive characterization of magnetic thin films and nanostructures by measurement of the emanating magnetic stray field. Established sensor probes operating at room temperature employ highly miniaturized spin-valve elements or semimetals, such as Bi. As the sensor layer structures are fabricated by patterning of planar thin films, their adaption to custom-made sensor probe geometries is highly challenging or impossible. Here we show how nanogranular ferromagnetic Hall devices fabricated by the direct-write method of focused electron beam induced deposition (FEBID) can be tailor-made for any given probe geometry. Furthermore, we demonstrate how the magnetic stray field sensitivity can be optimized in situ directly after direct-write nanofabrication of the sensor element. First proof-of-principle results on the use of this novel scanning Hall sensor are shown.
Comparative Study of the Photocatalytic Hydrogen Evolution over Cd1−xMnxS and CdS-β-Mn3O4-MnOOH Photocatalysts under Visible Light
Authors: Potapenko Ksenia O. , Kurenkova Anna Yu. , Bukhtiyarov Andrey V. , Gerasimov Evgeny Yu. , Cherepanova Svetlana V. , Kozlova Ekaterina A.
Published: February 2021

A series of solid solutions of cadmium and manganese sulfides, Cd1−xMnxS (x = 0–0.35), and composite photocatalysts, CdS-β-Mn3O4-MnOOH, were synthesized by precipitation with sodium sulfide from soluble cadmium and manganese salts with further hydrothermal treatment at 120 °C. The obtained photocatalysts were studied by the X-ray diffraction method (XRD), UV-vis diffuse reflectance spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and N2 low temperature adsorption. The photocatalysts were tested in hydrogen production using a Na2S/Na2SO3 aqueous solution under visible light (λ = 450 nm). It was shown for the first time that both kinds of photocatalysts possess high activity in hydrogen evolution under visible light. The solid solution Cd0.65Mn0.35S has an enhanced photocatalytic activity due to its valence and conduction band position tuning, whereas the CdS-β-Mn3O4-MnOOH (40–60 at% Mn) samples were active due to ternary heterojunction formation. Further, the composite CdS-β-Mn3O4-MnOOH photocatalyst had much higher stability in comparison to the Cd0.65Mn0.35S solid solution. The highest activity was 600 mmol g−1 h−1, and apparent quantum efficiency of 2.9% (λ = 450 nm) was possessed by the sample of CdS-β-Mn3O4-MnOOH (40 at% Mn).
Solvothermal Synthesis Combined with Design of Experiments—Optimization Approach for Magnetite Nanocrystal Clusters
Authors: Medinger Joelle , Nedyalkova Miroslava , Lattuada Marco
Published: February 2021

Magnetite nanocrystal clusters are being investigated for their potential applications in catalysis, magnetic separation, and drug delivery. Controlling their size and size distribution is of paramount importance and often requires tedious trial-and-error experimentation to determine the optimal conditions necessary to synthesize clusters with the desired properties. In this work, magnetite nanocrystal clusters were prepared via a one-pot solvothermal reaction, starting from an available protocol. In order to optimize the experimental factors controlling their synthesis, response surface methodology (RSM) was used. The size of nanocrystal clusters can be varied by changing the amount of stabilizer (tribasic sodium citrate) and the solvent ratio (diethylene glycol/ethylene glycol). Tuning the experimental conditions during the optimization process is often limited to changing one factor at a time, while the experimental design allows for variation of the factors’ levels simultaneously. The efficiency of the design to achieve maximum refinement for the independent variables (stabilizer amount, diethylene glycol/ethylene glycol (DEG/EG) ratio) towards the best conditions for spherical magnetite nanocrystal clusters with desirable size (measured by scanning electron microscopy and dynamic light scattering) and narrow size distribution as responses were proven and tested. The optimization procedure based on the RSM was then used in reverse mode to determine the factors from the knowledge of the response to predict the optimal synthesis conditions required to obtain a good size and size distribution. The RSM model was validated using a plethora of statistical methods. The design can facilitate the optimization procedure by overcoming the trial-and-error process with a systematic model-guided approach.
Ruling Factors in Cinnamaldehyde Hydrogenation: Activity and Selectivity of Pt-Mo Catalysts
Authors: Stucchi Marta , Manzoli Maela , Bossola Filippo , Villa Alberto , Prati Laura
Published: February 2021

To obtain selective hydrogenation catalysts with low noble metal content, two carbon-supported Mo-Pt bimetallic catalysts have been synthesized from two different molybdenum precursors, i.e., Na2MoO4 and (NH4)6Mo7O24. The results obtained by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) combined with the presence and strength of acid sites clarified the different catalytic behavior toward cinnamaldehyde hydrogenation. After impregnating the carbon support with Mo precursors, each sample was used either as is or treated at 400 °C in N2 flow, as support for Pt nanoparticles (NPs). The heating treatment before Pt deposition had a positive effect on the catalytic performance. Indeed, TEM analyses showed very homogeneously dispersed Pt NPs only when they were deposited on the heat-treated Mo/C supports, and XPS analyses revealed an increase in both the exposure and reduction of Pt, which was probably tuned by different MoO3/MoO2 ratios. Moreover, the different acid properties of the catalysts resulted in different selectivity.
MicroRNAs as Candidate Biomarkers for Alzheimer’s Disease
Authors: Kanach Colin , Blusztajn Jan K. , Fischer Andre , Delalle Ivana
Published: February 2021

The neurological damage of Alzheimer’s disease (AD) is thought to be irreversible upon onset of dementia-like symptoms, as it takes years to decades for occult pathologic changes to become symptomatic. It is thus necessary to identify individuals at risk for the development of the disease before symptoms manifest in order to provide early intervention. Surrogate markers are critical for early disease detection, stratification of patients in clinical trials, prediction of disease progression, evaluation of response to treatment, and also insight into pathomechanisms. Here, we review the evidence for a number of microRNAs that may serve as biomarkers with possible mechanistic insights into the AD pathophysiologic processes, years before the clinical manifestation of the disease.
Neuroscience and Neuroimmunology Solutions for Osteoarthritis Pain: Biological Drugs, Growth Factors, Peptides and Monoclonal Antibodies Targeting Peripheral Nerves
Authors: Mobasheri Ali
Published: February 2021

Neuroscience is a vast discipline that deals with the anatomy, biochemistry, molecular biology, physiology and pathophysiology of central and peripheral nerves. Advances made through basic, translational, and clinical research in the field of neuroscience have great potential for long-lasting and beneficial impacts on human and animal health. The emerging field of biological therapy is intersecting with the disciplines of neuroscience, orthopaedics and rheumatology, creating new horizons for interdisciplinary and applied research. Biological drugs, growth factors, therapeutic peptides and monoclonal antibodies are being developed and tested for the treatment of painful arthritic and rheumatic diseases. This concise communication focuses on the solutions provided by the fields of neuroscience and neuroimmunology for real-world clinical problems in the field of orthopaedics and rheumatology, focusing on synovial joint pain and the emerging biological treatments that specifically target pathways implicated in osteoarthritis pain in peripheral nerves.
Fate of Nitrogen from Artichoke (Cynara cardunculus L. var. scolymus (L.)) Crop Residues: A Review and Lysimeter Study
Authors: Akkal-Corfini Nouraya , Robin Paul , Menasseri-Aubry Safya , Corson Michael S. , Sévère Jean Paul , Collet Jean Michel , Morvan Thierry
Published: February 2021

The goal of the European Nitrate Directive 91/676/CEE is to mitigate or prevent water pollution associated with the nitrogen (N) cascade. Vegetable crops have a high risk of nitrate leaching during autumn and winter. Information about the fate of N from artichoke (Cynara cardunculus L. var. scolymus (L.)) residues is reviewed and then supplemented with a three-year study with 15N-labelled residues in an artichoke-cauliflower (Brassica oleracea L. cv. botrytis) rotation in six lysimeters. After three years, 6% of N in artichoke residues was leached, 8% was exported by crops, while 86% remained in the lysimeter. Summed over the rotation, 16% of artichoke-residue N was absorbed by artichoke and 14% by cauliflower. Total aboveground N uptake by all crops during the entire rotation ranged from 370 to 534 kg N ha−1, of which 207–311 kg N ha−1 returned to the soil as residues. Increasing N-recycling efficiency and reducing the risk of N leaching while conserving crop productivity requires capturing N mineralized from soil organic N. Cauliflower performs this capture effectively during the drainage period. A break crop should be introduced after the first and second harvests of artichoke to further synchronize N mineralization and uptake and reduce leaching risk during the rotation.
Enhanced Zinc Intake Protects against Oxidative Stress and Its Consequences in the Brain: A Study in an In Vivo Rat Model of Cadmium Exposure
Authors: Brzóska Małgorzata M. , Kozłowska Magdalena , Rogalska Joanna , Gałażyn-Sidorczuk Małgorzata , Roszczenko Alicja , Smereczański Nazar M.
Published: February 2021

We examined, in a rat model of moderate environmental human exposure to cadmium (Cd), whether the enhanced intake of zinc (Zn) may protect against Cd-caused destroying the oxidative/antioxidative balance and its consequences in the brain. The intoxication with Cd (5 mg/L, 6 months) weakened the enzymatic (superoxide dismutase, glutathione peroxidase, catalase) and non-enzymatic (total thiol groups, reduced glutathione) antioxidative barrier decreasing the total antioxidative status and increased the concentrations of pro-oxidants (hydrogen peroxide, myeloperoxidase) in this organ and its total oxidative status. These resulted in the development of oxidative stress and oxidative modifications of lipids and proteins. The co-administration of Zn (30 and 60 mg/L enhancing this element intake by 79% and 151%, respectively) importantly protected against Cd accumulation in the brain tissue and this xenobiotic-induced development of oxidative stress and oxidative damage to lipids and proteins. Moreover, this bioelement also prevented Cd-mediated oxidative stress evaluated in the serum. The favorable effect of Zn was caused by its independent action and interaction with Cd. Concluding, the enhancement of Zn intake under oral exposure to Cd may prevent the oxidative/antioxidative imbalance and oxidative stress in the brain and thus protect against injury of cellular macromolecules in the nervous system.
Mycobacterium tuberculosis Rv0580c Impedes the Intracellular Survival of Recombinant Mycobacteria, Manipulates the Cytokines, and Induces ER Stress and Apoptosis in Host Macrophages via NF-κB and p38/JNK Signaling
Authors: Ali Md Kaisar , Nzungize Lambert , Abbas Khushnood , Eckzechel Nzaou Stech Anomene , Abo-kadoum M. A. , Moure Ulrich Aymard Ekomi , Asaad Mohammed , Alam Aftab , Xu Junqi , Xie Jianping
Published: February 2021

The Mycobacterium tuberculosis (M. tb) genome encodes a large number of hypothetical proteins, which need to investigate their role in physiology, virulence, pathogenesis, and host interaction. To explore the role of hypothetical protein Rv0580c, we constructed the recombinant Mycobacterium smegmatis (M. smegmatis) strain, which expressed the Rv0580c protein heterologously. We observed that Rv0580c expressing M. smegmatis strain (Ms_Rv0580c) altered the colony morphology and increased the cell wall permeability, leading to this recombinant strain becoming susceptible to acidic stress, oxidative stress, cell wall-perturbing stress, and multiple antibiotics. The intracellular survival of Ms_Rv0580c was reduced in THP-1 macrophages. Ms_Rv0580c up-regulated the IFN-γ expression via NF-κB and JNK signaling, and down-regulated IL-10 expression via NF-κB signaling in THP-1 macrophages as compared to control. Moreover, Ms_Rv0580c up-regulated the expression of HIF-1α and ER stress marker genes via the NF-κB/JNK axis and JNK/p38 axis, respectively, and boosted the mitochondria-independent apoptosis in macrophages, which might be lead to eliminate the intracellular bacilli. This study explores the crucial role of Rv0580c protein in the physiology and novel host-pathogen interactions of mycobacteria.
Clinical Implications of Polymicrobial Synergism Effects on Antimicrobial Susceptibility
Authors: Little William , Black Caroline , Smith Allie Clinton
Published: February 2021

With the development of next generation sequencing technologies in recent years, it has been demonstrated that many human infectious processes, including chronic wounds, cystic fibrosis, and otitis media, are associated with a polymicrobial burden. Research has also demonstrated that polymicrobial infections tend to be associated with treatment failure and worse patient prognoses. Despite the importance of the polymicrobial nature of many infection states, the current clinical standard for determining antimicrobial susceptibility in the clinical laboratory is exclusively performed on unimicrobial suspensions. There is a growing body of research demonstrating that microorganisms in a polymicrobial environment can synergize their activities associated with a variety of outcomes, including changes to their antimicrobial susceptibility through both resistance and tolerance mechanisms. This review highlights the current body of work describing polymicrobial synergism, both inter- and intra-kingdom, impacting antimicrobial susceptibility. Given the importance of polymicrobial synergism in the clinical environment, a new system of determining antimicrobial susceptibility from polymicrobial infections may significantly impact patient treatment and outcomes.
Leptospiral Infection, Pathogenesis and Its Diagnosis—A Review
Authors: Samrot Antony V. , Sean Tan Chuan , Bhavya Karanam Sai , Sahithya Chamarthy Sai , Chan-drasekaran SaiPriya , Palanisamy Raji , Robinson Emilin Renitta , Subbiah Suresh Kumar , Mok Pooi Ling
Published: February 2021

Leptospirosis is a perplexing conundrum for many. In the existing literature, the pathophysiological mechanisms pertaining to leptospirosis is still not understood in full. Considered as a neglected tropical zoonotic disease, leptospirosis is culminating as a serious problem worldwide, seemingly existing as co-infections with various other unrelated diseases, including dengue and malaria. Misdiagnosis is also common as non-specific symptoms are documented extensively in the literature. This can easily lead to death, as the severe form of leptospirosis (Weil’s disease) manifests as a complex of systemic complications, especially renal failure. The virulence of Leptospira sp. is usually attributed to the outer membrane proteins, including LipL32. With an armament of virulence factors at their disposal, their ability to easily adhere, invade and replicate within cells calls for a swift refinement in research progress to establish their exact pathophysiological framework. As an effort to reconstitute the current knowledge on leptospirosis, the basis of leptospiral infection, including its risk factors, classification, morphology, transmission, pathogenesis, co-infections and clinical manifestations are highlighted in this review. The various diagnostic techniques are also outlined with emphasis on their respective pros and cons.