bluuebunny/crossref_metadata_2025 huggingface dataset:
Trans-Anethole Alleviates Subclinical Necro-Haemorrhagic Enteritis-Induced Intestinal Barrier Dysfunction and Intestinal Inflammation in Broilers
Authors: Yu Caiyun , Wang Dong , Tong Yichun , Li Qiming , Yang Weiren , Wang Tian , Yang Zaibin
Published: March 2022

This study investigated the alleviative potential of trans-anethole (TA) on the impaired intestinal barrier and intestinal inflammation and its regulatory effects on gut microbiota in broilers with subclinical necro-hemorrhagic enteritis (NE) challenge. Subclinical NE challenge led to a severe decline in the 21-day body weight (BW) and average daily gain (ADG), but an increase in feed conversion ratio (FCR) and intestinal lesion score of birds compared with controls (P < 0.05). Compared with the subclinical NE group, the TA administration group exhibited lower (P < 0.05) intestinal lesion score and crypt depth (CD), serum diamine oxidase activity, and D-lactate concentration, but higher (P < 0.05) intestinal tight junction protein expressions, villus height (VH), VH/CD, and numbers of proliferating cell nuclear antigen (PCNA)-positive cells. The administration of TA also inhibited (P < 0.05) the expression of intestinal pro-inflammatory cytokines including interleukin (IL)-1β, IL-8, interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) but increased (P < 0.05) jejunal IL-10 and secretory immunoglobulin A (sIgA) concentration. TA inclusion also led to a remarkable reduction of intestinal NF-kappa-B inhibitor alpha (IκBα) degradation and nuclear factor kappa beta (NF-κB) translocation. Moreover, TA modulated the cecal microbiota abundance and diversity of NE birds, as confirmed by reducing the phylum Firmicutes and genera Ruminococcaceae_UCG-014, Eubacterium_coprostanoligenes_group, and Ruminococcaceae_NK4A214_group when supplemented at 600 mg/kg and reducing genera Butyricicoccus, Oscillibacter, and Flavonifractor when supplemented at 400 mg/kg (P < 0.05). Supplementation of TA in broiler diets could alleviate subclinical NE infection by restoring intestinal barrier integrity, inhibiting NF-κB signaling pathway, and modulating gut microbiota. A 600-mg/kg dose of TA is the optimum concentration for ameliorating subclinical NE in broilers.
White Matter Hyperintensities and Functional Outcomes in Patients With Cerebral Hemorrhage: A Systematic Review and Meta-Analysis
Authors: You Qian , Yang Yi , Hu Hongtao
Published: March 2022

Background and Objectives

There are controversies about white matter hyperintensities (WMH) and the prognosis of spontaneous intracerebral hemorrhage. Our objective is to investigate the relationship between WMH and functional outcomes after intracerebral hemorrhage (ICH).

Methods

We systematically searched PubMed, EMBASE, and Cochrane Library databases from inception through August 10, 2021 without any restriction of countries. Articles investigating the relationship of WMH and functional outcomes as well as mortality of patients with spontaneous ICH were included. We extracted relevant data and evaluated the study quality with the Newcastle-Ottawa Scale. We pooled odds ratio (OR) for the presence and different severities of WMH with random effects models using STATA.

Results

A total of 10,584 patients with ICH in 18 studies were included in the analysis. Moderate/severe WMH were related to poor functional outcome [OR, 1.805, 95% confidence interval (CI), 1.320–2.469] and all-cause mortality (OR, 3.27, 95% CI, 2.07–5.18) after ICH. Besides, the increasing severity of WMH was also related to poor functional outcome (OR, 1.34, 95% CI, 1.17–1.53) and all-cause mortality (OR, 1.62, 95% CI, 1.39–1.90). The pooled data did not find the relationship between the presence of WMH and poor functional outcome (OR, 2.54, 95% CI, 0.91–7.05) after ICH. The results remained stable after adjusting for age, hematoma volume, stroke, and intraventricular hemorrhage.

Conclusion

We found moderate and severe WMH were related to poor functional outcomes and all-cause mortality after ICH. High-quality prospective studies are still needed.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42021278409.
Emerging Trends in Neuromodulation for Treatment of Drug-Resistant Epilepsy
Authors: Abouelleil Mohamed , Deshpande Nachiket , Ali Rushna
Published: March 2022

Epilepsy is a neurological disorder that affects more than 70 million people globally. A considerable proportion of epilepsy is resistant to anti-epileptic drugs (AED). For patients with drug-resistant epilepsy (DRE), who are not eligible for resective or ablative surgery, neuromodulation has been a palliative option. Since the approval of vagus nerve stimulation (VNS) in 1997, expansion to include other modalities, such as deep brain stimulation (DBS) and responsive neurostimulation (RNS), has led to improved seizure control in this population. In this article, we discuss the current updates and emerging trends on neuromodulation for epilepsy.
Metformin Promotes Differentiation and Attenuates H2O2-Induced Oxidative Damage of Osteoblasts via the PI3K/AKT/Nrf2/HO-1 Pathway
Authors: Yang Keda , Cao Fangming , Qiu Shui , Jiang Wen , Tao Lin , Zhu Yue
Published: March 2022

At present, the drug treatment of osteoporosis is mostly focused on inhibiting osteoclastogenesis, which has relatively poor effects. Metformin is a drug that can potentially promote osteogenic differentiation and improve bone mass in postmenopausal women. We aimed to detect the molecular mechanism underlying the osteogenic effect of metformin. Our study indicated that metformin obviously increased the Alkaline phosphatase activity and expression of osteogenic marker genes at the mRNA and protein levels. The PI3K/AKT signaling pathway was revealed to play an essential role in the metformin-induced osteogenic process, as shown by RNA sequencing. We added LY294002 to inhibit the PI3K/AKT pathway, and the results indicated that the osteogenic effect of metformin was also blocked. Additionally, the sequencing data also indicated oxidation-reduction reaction was involved in the osteogenic process of osteoblasts. We used H2O2 to mimic the oxidative damage of osteoblasts, but metformin could attenuate it. Antioxidative Nrf2/HO-1 pathway, regarded as the downstream of PI3K/AKT pathway, was modulated by metformin in the protective process. We also revealed that metformin could improve bone mass and oxidative level of OVX mice. In conclusion, our study revealed that metformin promoted osteogenic differentiation and H2O2-induced oxidative damage of osteoblasts via the PI3K/AKT/Nrf2/HO-1 pathway.
Adenosine A2A Receptor: A New Neuroprotective Target in Light-Induced Retinal Degeneration
Authors: Soliño Manuel , Larrayoz Ignacio M. , López Ester María , Rey-Funes Manuel , Bareiro Mariana , Loidl Cesar Fabián , Girardi Elena , Martínez Alfredo , López-Costa Juan José
Published: March 2022

Continuous illumination induces the degeneration of photoreceptors. This animal model of light-induced retinal degeneration resembles many characteristics of human degenerative diseases of the outer retina, such as age-related macular degeneration. This work aimed to evaluate the potential neuroprotective effect of the modulation of adenosine A2A receptor in the model of light-induced retinal degeneration. Sprague-Dawley rats were intravitreally injected in the right eye with either CGS 21680, an adenosine A2A receptor agonist, or SCH 58261, an adenosine A2A receptor antagonist. Contralateral eyes were injected with respective vehicles as control. Then, rats were subjected to continuous illumination (12,000 lux) for 24 h. Retinas were processed by glial fibrillary acidic protein (GFAP) immunohistochemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique, Western blotting (WB), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Another group of rats was subjected to functional studies by electroretinography. Animals treated with CGS21680 showed a significant increase of apoptotic nuclei in the outer nuclear layer and a significant increase of GFAP immunoreactive area of the retinas but did not alter WB nor electroretinography results. qRT-PCR showed that CGS 21680 significantly increased the expression of interleukin-1β. On the opposite, SCH 58261 significantly decreased apoptotic nuclei in the outer nuclear layer and GFAP immunoreactive area of the retinas. It also significantly decreased GFAP and activated caspase-3 levels as measured by WB and preserved retinal function, as treated eyes showed significantly greater amplitudes of a- and b-waves and oscillatory potentials. qRT-PCR revealed that SCH 58261 significantly decreased the expression of tumor necrosis factor-α. These results show that the blockade of the A2A receptor before the start of the pathogenic process is neuroprotective, as it prevents light-induced retinal damage. The use of A2A receptor antagonists deserves to be evaluated in retinal degenerative diseases.
Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes
Authors: Chuberre Coralie , Chan Philippe , Walet-Balieu Marie-Laure , Thiébert François , Burel Carole , Hardouin Julie , Gügi Bruno , Bardor Muriel
Published: March 2022

Phaeodactylum tricornutum is an atypical diatom since it can display three main morphotypes: fusiform, triradiate, and oval. Such pleomorphism is possible thanks to an original metabolism, which is tightly regulated in order to acclimate to environmental conditions. Currently, studies dedicated to the comparison of each morphotype issued from one specific strain are scarce and little information is available regarding the physiological significance of this morphogenesis. In this study, we performed a comparative proteomic analysis of the three morphotypes from P. tricornutum. Cultures highly enriched in one dominant morphotype (fusiform, triradiate, or oval) of P. tricornutum Pt3 strain were used. Pairwise comparisons highlighted biological processes, which are up- and down-regulated in the oval (e.g., purine and cellular amino acid metabolism) and triradiate morphotypes (e.g., oxido-reduction and glycolytic processes) compared to the fusiform one used as a reference. Intersection analysis allowed us to identify the specific features of the oval morphotype. Results from this study confirmed previous transcriptomic RNA sequencing observation showing that the oval cells present a distinct metabolism with specific protein enrichment compared to fusiform and triradiate cells. Finally, the analysis of the secretome of each morphotype was also performed.
The Role of Paternal Parenting and Co-parenting Quality in Children’s Academic Self-Efficacy
Authors: Kara Demet , Sümer Nebi
Published: March 2022

This study explored the unique effect of fathers’ parenting behaviors and the quality of co-parenting described as the degree of consistency between paternal and maternal parenting behaviors on children’s academic self-efficacy. The power of both pancultural parenting behaviors (i.e., emotional warmth and rejection) and specific parenting controlling behaviors that are relatively common in Turkish culture (i.e., intrusion and guilt induction) in predicting academic self-efficacy was tested. A total of 1,931 children completed measures of parenting behaviors and academic self-efficacy in math and literature courses in their school. Overall, girls reported higher levels of literature self-efficacy, whereas boys reported higher levels of math self-efficacy. Compared to boys, girls perceived higher levels of positive parenting behaviors from both their fathers and mothers. The results of the regression analyses showed that, whereas father warmth had stronger effects on boys’ math self-efficacy, mother warmth had stronger effects on girls’ literature self-efficacy. Examination of the effects of co-parenting quality demonstrated that children with positively consistent parents (i.e., both parents having high positive and low negative parenting behaviors) reported the highest level of academic self-efficacy, whereas those having negatively consistent parents had the lowest level of academic self-efficacy. Analyses on inconsistent co-parenting, however, yielded compensatory effects, which were similar to positively consistent parents, and deterioration effects, which were similar to negatively consistent parents depending on the gender of parent and child, domain of parenting behavior, and academic efficacy. This study contributed to the current literature by showing the unique role of fathers over and beyond mothers, and confirmed the importance of positive parenting and parenting consistency in promoting children’s academic efficacy. Cultural and practical implications of the findings were discussed.
Categorical Versus Graded Beliefs
Authors: Dietrich Franz
Published: March 2022

This essay discusses the difficulty to reconcile two paradigms about beliefs: the binary or categorical paradigm of yes/no beliefs and the probabilistic paradigm of degrees of belief. The possibility for someone to hold beliefs of both types simultaneously is challenged by the lottery paradox, and more recently by a general impossibility theorem by Dietrich and List. The nature, relevance, and implications of the tension are explained and assessed.
Internet Addiction and Its Associated Factors Among African High School and University Students: Systematic Review and Meta-Analysis
Authors: Zewde Edgeit Abebe , Tolossa Tadesse , Tiruneh Sofonyas Abebaw , Azanaw Melkalem Mamuye , Yitbarek Getachew Yideg , Admasu Fitalew Tadele , Ayehu Gashaw Walle , Amare Tadeg Jemere , Abebe Endeshaw Chekol , Muche Zelalem Tilahun , Fentie Tigabnesh Assfaw , Zemene Melkamu Aderajew , Melaku Metages Damite
Published: March 2022

Introduction

Internet addiction is characterized by excessive and uncontrolled use of the internet affecting everyday life. Adolescents are the primary risk group for internet addiction. Data on internet addiction is lacking in Africa. Thus, this review aimed to determine the pooled prevalence of internet addiction and its associated factors among high school and university students in Africa.

Methods

A comprehensive literature search was conducted using electronic databases (PubMed/MEDLINE, Web of science, Hinari, and Google scholar) to locate potential studies. Heterogeneity between studies was checked using Cochrane Q test statistics and I2 test statistics and small-study effects were checked using Egger's statistical test at a 5% significance level. A sensitivity analysis was performed. A random-effects model was employed to estimate the pooled prevalence and associated factors of internet addiction among students. The primary outcome of measure of this review was the prevalence of internet addiction and the secondary outcome of measures are the factors associated with internet addiction.

Results

A total of 5,562 studies were identified among the five databases. Of these, 28 studies from 10 countries with 14,946 high school and university students were included in this review. The overall pooled prevalence of internet addiction among the students was 34.53% [95% Confidence Interval (CI): 26.83, 42.23, I2 = 99.20%]. Male sex [Pooled Odds Ratio (POR) = 1.92, 95% CI:1.43, 2.57 I2 = 0.00], urban residence (POR = 2.32, 95% CI:1.19, 4.53, I2 = 59.39%), and duration of daily internet use for more than 4 h (POR = 2.25, 95% CI:1.20, 4.21, I2 = 0.00%, were significantly associated with internet addiction among adolescents.

Conclusion

Almost one-third of university and high school students in Africa are addicted to the internet. Male students, those from urban areas, and those who use the internet for more than 4 h per day have higher odds of internet addiction. Thus, we recommend that health planners and policymakers pay attention to the use of the internet and internet addiction in Africa.
A Study on Dynamic Characteristics of Thin-Walled Cylindrical Cavities with a Large Aspect Ratio
Authors: Xu Fangfang , Li Hong , Zhang Dongxu
Published: March 2022

The unstable combustion problem in small-sized solid rocket engines with a large aspect ratio is so complicated that its causes remain unclear. In this study, the coupled vibration between the sound field and shell in the engines was proposed as a possible cause. A solid rocket engine structure was abstracted into a multilayer thin-walled cylindrical cavity in this study, followed by the theoretical calculation and simulation calculation of its inherent frequency. Next, a thin-walled cylindrical cavity fluid-solid coupling experimental platform with the function of modal measurement was established to verify the accuracy of simulated modes for the shell structure and acoustic cavity. Then, the mode of the finite element model (FEM) for the solid rocket engine was theoretically calculated and simulated, accompanied by finite element calculation and experiment of the acoustic mode of the internal acoustic cavity. Subsequently, the engine mode was compared with the acoustic mode of the internal acoustic cavity. On this basis, a new cause for the damage and disintegration of the solid rocket engines in the final working stage was revealed. Moreover, a brand-new idea of inhibiting the pressure oscillation-induced unstable combustion in the solid rocket engines was put forward.
Validation of Molecular Markers of Barley Net Blotch Resistance Loci on Chromosome 3H for Marker-Assisted Selection
Authors: Afanasenko Olga , Rozanova Irina , Gofman Anastasiia , Lashina Nina , Novakazi Fluturë , Mironenko Nina , Baranova Olga , Zubkovich Alexandr
Published: March 2022

The most widespread and harmful disease of barley is net form of net blotch caused by the ascomycete Pyrenophora teres f. teres Drechsler (Ptt). A cost effective and environmentally sustainable strategy for barley protection against Ptt is to develop barley cultivars possessing genetic resistance. In previous GWA analysis, we identified SNP-markers associated with a resistance locus on chromosome 3H in the interval of 45.82–54.53 cM. These SNPs have been described previously in the literature to be located within the same region of chromosome 3H. The aim of the study was to validate QTL markers controlling resistance to Ptt on chromosome 3H in this region by KASP genotyping in four F2 populations of crosses between the resistant cultivars, Morex, Fox, and Zolo, and the accession, Local k-21578, with the susceptible barley cv. Gesine and in a doubled haploid (DH) population of Canadian Lake Shore (CLS)/Harrington. Eleven of fifteen studied markers showed high efficacy (97.5–100%) for co-segregation with resistance to Ptt in the DH population, CLS/Harrington. Three of these markers located at 54.53 cM and one at 51.27 cM were effective in two F2 populations of crosses of Morex and Fox with susceptible cv. Gesine. These markers are also located close to each other on the physical map (442,203,921–443,119,491 bp). Apparently, in cultivars, CLS, Morex, and Fox, resistance to Ptt is determined by the same locus. Markers JHI-Hv50k-2016-166392 (47.1 cM, 112,536,071 bp), Clone ID 3255462_1 (51.63 cM, 363,531,898 bp), and Clone ID 3255462_2 (51.63 cM, 363,531,871 bp) showed high efficacy in the DH population and in the F2 population, Local k-21578/Gesine. Apparently, at least two loci controlling Ptt resistance exist in the chromosome region of 47.0–54.3 cM: one at 46.0–48.44 cM and another at 51.27–54.8 cM. These regions were found to harbor several genes involved in important plant functions, including disease response and signaling pathways. Allele-specific PCR markers were developed based on the KASP assay data and tested on six resistant, two moderately resistant, and two susceptible barley genotypes. Four markers were found to be effective to differentiate susceptible and resistant barley genotypes. The KASP and allele-specific PCR markers associated with Ptt resistance on chromosome 3H will be useful for pyramiding resistance QTLs in barley marker-assisted selection.
Strengthened Assimilate Transport Improves Yield and Quality of Super Rice
Authors: Chen Tingting , Yang Xueqin , Fu Weimeng , Li Guangyan , Feng Baohua , Fu Guanfu , Tao Longxing
Published: March 2022

Rice varieties with ultra-high yields play an important role in grain production and global food security. However, little information is available on the source–sink relationships that underpin the grain quality and ultra-high-yield properties. Photosynthesis, carbohydrate accumulation and allocation, vascular bundle morphology, and nutrient uptake and characteristics were, therefore, compared in two ‘super rice’ varieties: Yongyou9 (control) and Yongyou12 (ultra-high yield) that differ in grain production. Yongyou12 had a significantly higher (18.8–21.4%) grain yield than Yongyou9, together with a substantial improvement in appearance-related qualities. The total dry weight and the ratio of panicle dry weight to total dry weight were significantly higher in Yongyou12 than Yongyou9, suggesting that the improved seed traits were related to higher assimilate accumulation and allocation in the ultra-high-yield variety. Yongyou12 had larger vascular bundles and greater numbers of vascular bundles in the panicle-neck internode, as well as higher levels of SUT1, SUT2, and CIN2 transcripts in the grains than Yongyou9. The contents of nitrogen, phosphorous, and potassium were similar in Yongyou12 and Yongyou9. We concluded that assimilate transport and nutrient utilization efficiency are the main factors underlying the higher yield and quality traits of the super rice variety Yongyou12.
Humic Substances: A Novel Eco-Friendly Fertilizer
Authors: Roulia Maria
Published: March 2022

Humic substances (HS) are macromolecular organic materials ubiquitous in both aquatic and terrestrial systems, effective electron shuttling compounds providing macro- and microelements in organochelate form, and play an important role in determining the mobility and bioavailability of organic/inorganic nutrients and mineral fertilizers [...]
How Could Precision Irrigation Based on Daily Trunk Growth Improve Super High-Density Olive Orchard Irrigation Efficiency?
Authors: Arbizu-Milagro Julia , Castillo-Ruiz Francisco J. , Tascón Alberto , Peña Jose M.
Published: March 2022

Water deficit, especially during summer, is currently one of the most important stress factors that influence olive oil production in olive orchards. A precision irrigation strategy, based on daily trunk growth, was assessed and compared with one continuous deficit, one full irrigation, and two different regulated deficit irrigation strategies. All of them were tested in a super high-density olive orchard located in northeast Spain, in which oil production, main oil production components, applied irrigation water, and water productivity were assessed. For this purpose, the crop was monitored from budding to harvesting, mainly during the summer months in which the Precision strategy only applied water after two days of negative daily trunk growth. Maximum monthly water savings for the Precision strategy reached 91.8%, compared with full irrigation, while major annual mean water savings reached 50% for the continuous deficit strategy and 31.2% for the Precision strategy, which also reduced irrigation events by up to 19.7%, compared with the full irrigation strategy. Oil production and oil production components varied depending on the irrigation strategies providing the Control, one of the regulated deficit irrigations, and Precision higher values than the other strategies; oil yield results differ, nonetheless. The Precision strategy showed an overall better performance. Despite this, it did not achieve the highest water saving, it achieved higher water productivity.
Comparison of Chemical and Biological Wireworm Control Options in Serbian Sunflower Fields and a Proposition for a Refined Wireworm Damage Assessment
Authors: Gvozdenac Sonja , Milovac Željko , Vidal Stefan , Crvenković Zagorka Lozanov , Papuga Ivana Štajner , Franeta Filip , Ovuka Jelena , Cvejić Sandra
Published: March 2022

Recent European Union regulations aim at reducing the use of chemical pesticides in agriculture. In sunflower production in Serbia, the use of synthetic insecticides for soil and seed treatments has become a prevailing practice for wireworm (Coleoptera: Elateridae) control. However, a number of compounds efficiently used as seed treatments have been phased out. This work aimed at assessing the potential of an environmentally friendly “Attract and Kill” strategy (A&K) for controlling wireworms in sunflower in comparison to conventional insecticides. The experiments were carried out in 2018, 2019 and 2021 involving (a) soil treatments with ATTRACAP® and a Force 1.5 G; (b) seed treatments with Sonido, Force 20 CS, Lumiposa and Buteo Start 480 FS; (c) controls (i.e., untreated seeds). The efficacy of the treatments was assessed based on the plant density and emerging plant damage (%). A damage rating scale (levels 0–5) was created, aiming for a more reliable and concrete interpretation of the results. Data were processed using binomial and multinomial regressions, followed by modelling of the damage and calculating the odds of damage occurrence, depending on the applied insecticide. In all experimental years and at all localities, the Force 20 CS and/or Force 1.5 G treatments resulted in the highest plant density and the lowest percentage of plant damage. ATTRACAP® showed good effectiveness, since plant density and percentage of damaged plants were at the same level of significance as the commonly used conventional insecticides in sunflower production (Sonido for seed treatment and Force 1.5 G for soil treatment). Thus, the A&K strategy was efficient in controlling wireworms at conditions of low abundance, based on three-year experimental results. Although the damage rating scale enabled a clear differentiation of plant damage caused by wireworms responsible for reduced plant density, more reliable models were obtained by binomial regressions, classifying plants as damaged or undamaged.
Mechanical Pruning of ‘Clemenules’ Mandarins in Spain: Yield Effects and Economic Analysis
Authors: Fonte Alberto , Torregrosa Antonio , Garcerá Cruz , Mateu Guillermo , Chueca Patricia
Published: March 2022

Pruning is one of the most expensive tasks in citrus production, and its mechanization could increase the productivity and competitiveness of citrus farms. The effect of mechanical pruning on yield depends on the variety, crop condition, and location; among other factors. The ‘Clemenules’ mandarin variety is one of the most important ones; therefore, the aim of this work was to study the effects of twelve pruning strategies on ‘Clemenules’ yield over four years, and to conduct an economic analysis. These strategies included fully manual pruning, annual alternation of mechanical/manual pruning, mechanical pruning with manual follow-up, and fully mechanical pruning with different types of cuttings. The results showed that pruning strategies affected the yield and fruit size in some years of the study but not when the four years overall are considered. Strategies that annually alternated mechanical pruning with manual pruning had higher yields than the fully mechanical approaches, and similar yields to the manual and control (no pruning) strategies. Furthermore, although the mechanical pruning costs per hectare were as minimum two times less than those with manual pruning, higher net value was achieved with the manual pruning approach followed by alternate annual mechanical/manual pruning. In conclusion, after four years of study, the pruning strategy with the best global results for ‘Clemenules’ mandarin was that which involved annually alternating mechanical (topping and one-sided hedging) and manual pruning. This strategy allows maintaining regular tree dimensions without dry branches inside.
Diversity of Plum Stones Based on Image Texture Parameters and Machine Learning Algorithms
Authors: Ropelewska Ewa
Published: March 2022

The objective of this study was to evaluate the usefulness of machine learning based on image texture parameters to discriminate plum stone cultivars. The plums of cultivars ‘Emper’, ‘Kalipso’, and ‘Polinka’ were sampled. For each cultivar, one hundred images of plum stones were acquired using a digital camera. Processing of the plum stone images included the conversion of the images to individual color channels, image segmentation, region of interest (ROI) determination, and texture parameter extraction. Then, the discriminant analysis, including the texture selection and building discriminative models for the evaluation of the diversity of the plum stone cultivars, was carried out. The obtained results of discrimination of plum stone cultivars were very accurate and confirmed the effectiveness of image processing to evaluate the cultivar diversity. The most satisfactory results, reaching 96.67% for the average accuracy for three cultivars (97% for ‘Emper’, ‘Kalipso’, and 96% for ‘Polinka’), were obtained for the models built based on combined textures selected from all the color channels using the IBk classifier. The developed procedure can be of practical importance for the correct identification of plum stone cultivars and avoiding their mixing to preserve cultivar uniformity.
Ensifer meliloti L6-AK89, an Effective Inoculant of Medicago lupulina Varieties: Phenotypic and Deep-Genome Screening
Authors: Roumiantseva Marina L. , Vladimirova Maria E. , Saksaganskaia Alla S. , Muntyan Victoria S. , Kozlova Alexandra P. , Afonin Alexey M. , Baturina Olga A. , Simarov Boris V.
Published: March 2022

This paper presents a deep analysis of the accessory genome of an economically promising strain of Ensifer (Sinorhizobium) meliloti, L6-AK89, obtained as a result of next-generation high-throughput sequencing (MiSeq, MinIon). Strain L6-AK89 is a StrR mutant of the native strain CIAM1775, a symbiont of Medicago lupulina that adapted to a saline and arid habitat in NW Kazakhstan. CIAM1775 is an effective inoculant of M. lupulina cv. Mira (fodder type standard), cultivated on moderately acid soils in the NW agricultural region of Russia. Strain L6-AK89 makes it possible to obtain the expected high (>150%) increases in dry mass of the same plant variety in plant tests. The L6-AK89 genome has an increased proportion of sequences related to the accessory elements relative to reference strain Rm1021, 7.4% versus 4.8%. A set of 53 nod/noe/nol/nif/fdx/fix genes and 32 genes involved in stress tolerance together with 16S rRNA and recA–atpD–glnII–gyrB–dnaJ were evaluated. The high symbiotic efficiency of L6-АК89 with hop clover is most likely due to unique features of its genome, in combination with structural differences in its nod and stress-related genes, as well as unique clusters of quorum-sensing genes and osmoprotector synthesis.
Effect of a Novel Hydroxybenzoic Acid Based Mitochondria Directed Antioxidant Molecule on Bovine Sperm Function and Embryo Production
Authors: Santos João Campos , Marques Carla Cruz , Baptista Maria Conceição , Pimenta Jorge , Teixeira José , Montezinho Liliana , Cagide Fernando , Borges Fernanda , Oliveira Paulo J. , Pereira Rosa M. L. N.
Published: March 2022

Sperm cells are particularly vulnerable to reactive oxygen species (ROS), impairing their fertilizing ability. Our objective was to study the effect of a novel mitochondrial-directed antioxidant, AntiOxBEN2, on bovine sperm function. This antioxidant was added to the semen capacitation medium (CAP), during the swim-up process, and to the fertilization medium (FERT) during the co-incubation of matured oocytes and capacitated spermatozoa, in concentrations of 0 (control), 1, and 10 µM. After the swim-up, sperm motility (CASA and visual analysis), vitality (eosin-nigrosin), mitochondrial membrane potential (JC1), intracellular ROS, adenosine triphosphate (ATP) levels, and basal metabolism (Seahorse Xfe96) were evaluated. Embryo development and quality were also assessed. Higher cleavage rates were obtained when 1 µM AntiOxBEN2 were added to CAP and FERT media (compared to control, p < 0.04). A positive effect of AntiOxBEN2 on intracellular ROS reduction (p = 0.01), on the increment of mitochondrial membrane potential (p ≤ 0.003) and, consequently, on the sperm quality was identified. However, the highest dose impaired progressive motility, ATP production, and the number of produced embryos. The results demonstrate a beneficial effect of AntiOxBEN2 (1 µM) on sperm capacitation and fertilization processes, thus improving embryonic development. This may constitute a putative novel therapeutic strategy to improve the outcomes of assisted reproductive techniques (ART).
All-You-Can-Eat: Influence of Proximity to Maize Gardens on the Wild Diet and the Forest Activities of the Sebitoli Chimpanzee Community in Kibale National Park
Authors: Couturier Chloé , Bortolamiol Sarah , Ortmann Sylvia , Okimat John-Paul , Asalu Edward , Krief Sabrina
Published: March 2022

Frugivorous primates have developed several strategies to deal with wild fruit scarcity, such as modifying their activity budget or enlarging their diet. Agricultural expansion threatens primate habitats and populations (e.g., disease transmission, agrochemical exposure), but it also increases crop feeding opportunities. We aimed at understanding whether maize presence close to the natural habitat of chimpanzees, a threatened species, would lead to significant behavioral modifications. We monitored 20 chimpanzees over 37 months in Kibale National Park, Uganda, with maize gardens at the forest edge. Based on focal nest-to-nest data, we analyzed their diet, activity budget, and energy balance depending on wild fruit and maize availability. We found that the Sebitoli area is a highly nutritive habitat for chimpanzees, with large and caloric wild fruits available all year long. The chimpanzees opportunistically consume maize and exploit it by resting less during maize season. However, no significant variation was found in daily paths and energy expenditures according to maize availability. No behavioral or energy modification was observed regarding wild resources either. Despite the availability of nutritious domestic resources, chimpanzees still exploit wild fruits and do not limit their movements. Thus, their contribution to seed dispersal and forest regeneration in this area is not affected.
Alpha-Tocopherol, a Powerful Molecule, Leads to the Formation of Oxylipins in Polyunsaturated Oils Differently to the Temperature Increase: A Detailed Study by Proton Nuclear Magnetic Resonance of Walnut Oil Oxidation
Authors: Caño-Ochoa Sofía del , Ruiz-Aracama Ainhoa , Guillén María D.
Published: March 2022

Lipid oxidation causes food degradation and the formation of toxic compounds. Therefore, the addition to foods of compounds able to avoid, delay or minimize this degradative process is a commonly used strategy. Nevertheless, neither the identity of most of the formed compounds in this complex process nor the way in which their formation is affected by the strategy used are well known. In this context, the effect the temperature increase and the enrichment level in alpha-tocopherol on the evolution of the walnut oil oxidation, as a model of an oil rich in polyunsaturated omega-6 acyl groups, submitted to storage conditions, are tackled by 1H NMR. The study has allowed knowing the degradation kinetic of both the oil acyl groups and alpha-tocopherol, the identification of a very high number of oxylipins and the kinetic of their formation. The temperature increase accelerates the formation of all oxylipins, favouring the formation of hydroperoxy conjugated E,E-dienes and related derivatives versus that of the Z,E-isomers. The enrichment in alpha-tocopherol accelerates the formation of hydroperoxy conjugated Z,E-dienes and related derivatives, and delays in relation to the formation of the former that of the E,E-isomers and related derivatives, hindering, to a certain extent, the formation of the latter in line with the enrichment level.
Anti-Inflammatory Activity of Monosubstituted Xestoquinone Analogues from the Marine Sponge Neopetrosia compacta
Authors: Susana Shalice R. , Salvador-Reyes Lilibeth A.
Published: March 2022

Chronic inflammation is recognized as a contributor to multiple chronic diseases, such as cancer, cardiovascular, and autoimmune disorders. Here, a natural products-initiated discovery of anti-inflammatory agents from marine sponges was undertaken. From the screening of 231 crude extracts, a total of 30 extracts showed anti-inflammatory activity with no direct cytotoxic effects at 50 μg/mL on RAW 264.7 (ATCC®TIB-71™) murine macrophage cells stimulated with 1 μg/mL lipopolysaccharide (LPS). Bioactivity-guided purification of the anti-inflammatory extract from the sponge Neopetrosia compacta led to the isolation of xestoquinone (1), adociaquinone B (2), adociaquinone A (3), 14-hydroxymethylxestoquinone (4), 15-hydroxymethylxestoquinone (5), and an inseparable 2:1 mixture of 14-methoxyxestoquinone and 15-methoxyxestoquinone (6). Compounds 1–6 caused a concentration-dependent reduction of nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells, with 4–6 having low micromolar IC50 and acceptable selectivity index. Gene expression analysis using qRT-PCR showed that 1, 5, and 6 downregulated Il1b and Nos2 expression by 2.1- to 14.8-fold relative to the solvent control at 10 μM. Xestoquinone (1) and monosubstituted analogues (4–6), but not the disubstituted adociaquinones (2 and 3), caused Nrf2 activation in a luciferase reporter MCF7 stable cells. Compounds 5 and 6 caused a modest increase in Nqo1 gene expression at 10 μM. The anti-inflammatory activity of xestoquinone (1) and monosubstituted analogues (4–6) may, in part, be mediated by Nrf2 activation, leading to attenuation of inflammatory mediators such as IL-1β and NOS2.
Hypothiocyanous Acid Disrupts the Barrier Function of Brain Endothelial Cells
Authors: van Leeuwen Eveline , Hampton Mark B. , Smyth Leon C. D.
Published: March 2022

Inflammation is a common feature of neurological diseases. During neuroinflammation, neutrophils are recruited to the brain vasculature, where myeloperoxidase can produce hypochlorous acid and the less well-studied oxidant hypothiocyanous acid (HOSCN). In this study, we exposed primary brain endothelial cells (BECs) to HOSCN and observed a rapid loss of transendothelial electrical resistance (TEER) at sublethal concentrations. Decreased barrier function was associated with a loss of tight junctions at cellular contacts and a concomitant loss of dynamic microtubules. Both tight junction and cytoskeletal disruptions were visible within 30 min of exposure, whereas significant loss of TEER took more than 1 h. The removal of the HOSCN after 30 min prevented subsequent barrier dysfunction. These results indicate that BECs are sensitive to HOSCN, resulting in the eventual loss of barrier function. We hypothesise that this mechanism may be relevant in neutrophil transmigration, with HOSCN facilitating blood–brain barrier opening at the sites of egress. Furthermore, this mechanism may be a way through which neutrophils, residing in the vasculature, can influence neuroinflammation in diseases.
The Retinal Renin-Angiotensin-Aldosterone System: Implications for Glaucoma
Authors: Hirooka Kazuyuki , Kiuchi Yoshiaki
Published: March 2022

Aldosterone is one of the main effectors of the renin-angiotensin-aldosterone system (RAAS) along with having roles in hypertension, and cardiovascular and renal diseases. Recent evidence has also shown the presence of an active local RAAS within the human eye. It has been shown that at 12 h after a retinal ischemia-reperfusion injury, there is an upregulation of the protein levels of angiotensin II type 1 receptor (AT1-R) in the retina. Furthermore, at 12 h after reperfusion, there is an increase in reactive oxygen species (ROS) production in the retina that is mediated via an NADPH oxidase pathway. This ischemia-reperfusion injury-induced increase of retinal ROS levels and NADPH oxidase expression can be prevented by the administration of an AT1-R antagonist. This suggests that one of the main retinal ischemic injury pathways is via the local RAAS. It has also been reported that progressive retinal ganglion cell loss and glaucomatous optic nerve degeneration without elevated intraocular pressure occur after administration of local or systemic aldosterone. Elucidation of glaucoma pathogenesis, especially normal-tension glaucoma (NTG) subtype by our current animal model can be used for identifying potential therapeutic targets. Based on these results, we are further evaluating NTG prevalence among primary aldosteronism patients.
Efficacy of Four In Vitro Fungicides for Control of Wilting of Strawberry Crops in Puebla-Mexico
Authors: Coronel Alba Cruz , Parraguirre Lezama Conrado , Pacheco Hernández Yesenia , Santiago Trinidad Olga , Rivera Tapia Antonio , Romero-Arenas Omar
Published: March 2022

Strawberry wilt is an established disease of strawberry crops caused by fungus Fusarium solani. In Mexico, strawberry cultivation represents an important productive activity for several rural areas; however, wilt disease affects producers economically. The objectives of this research were: (a) to identify and morphologically characterize strain “MA-FC120” associated with root rot and wilting of strawberry crops in Santa Cruz Analco, municipality of San Salvador el Verde, Puebla-Mexico; (b) to evaluate the potential of single and multiple applications of four broad-spectrum fungicides used against F. solani in vitro. Plant tissue samples were collected from strawberry crops in Puebla-Mexico with presence of symptoms of desiccation and root rot. Strain “MA-FC120” was identified as F. solani, being the causal agent of wilt and root rot in strawberry plants from Santa Cruz Analco. Fungicide Benomyl 50® showed the highest percentage of inhibition on F. solani (100%) under in vitro conditions. The fungicide Mancosol 80® and Talonil 75® at low concentration (600 and 450 mg L−1) showed no toxicity, being harmless to strain MA-FC120. However, fungicide Talonil 75® showed slight toxicity at the dose recommended by the manufacturer and moderate toxicity in high concentration (1350 mg L−1). Likewise, Captan 50® in its three concentrations evaluated showed slight toxicity, obtaining around 50% on the classification scale established by International Organization for Biological Control (IOBC).
Recent Applications of Artificial Intelligence in Radiotherapy: Where We Are and Beyond
Authors: Santoro Miriam , Strolin Silvia , Paolani Giulia , Della Gala Giuseppe , Bartoloni Alessandro , Giacometti Cinzia , Ammendolia Ilario , Morganti Alessio Giuseppe , Strigari Lidia
Published: March 2022

In recent decades, artificial intelligence (AI) tools have been applied in many medical fields, opening the possibility of finding novel solutions for managing very complex and multifactorial problems, such as those commonly encountered in radiotherapy (RT). We conducted a PubMed and Scopus search to identify the AI application field in RT limited to the last four years. In total, 1824 original papers were identified, and 921 were analyzed by considering the phase of the RT workflow according to the applied AI approaches. AI permits the processing of large quantities of information, data, and images stored in RT oncology information systems, a process that is not manageable for individuals or groups. AI allows the iterative application of complex tasks in large datasets (e.g., delineating normal tissues or finding optimal planning solutions) and might support the entire community working in the various sectors of RT, as summarized in this overview. AI-based tools are now on the roadmap for RT and have been applied to the entire workflow, mainly for segmentation, the generation of synthetic images, and outcome prediction. Several concerns were raised, including the need for harmonization while overcoming ethical, legal, and skill barriers.
Resource Allocation for Throughput versus Fairness Trade-Offs under User Data Rate Fairness in NOMA Systems in 5G Networks
Authors: Abuajwa Osama , Roslee Mardeni , Yusoff Zubaida Binti , Chuan Lee Loo , Leong Pang Wai
Published: March 2022

In this work, we present a resource allocation scheme for managing trade-offs between total throughput maximisation and system fairness in a non-orthogonal multiple access (NOMA) system for 5G networks. Our proposed approach is designed to improve throughput and fairness as performance metrics of NOMA in 5G networks. We apply integer linear programming for user pairing and adopt particle swarm optimisation as the power allocation scheme for reducing resource allocation complexity. To formulate the multi-objective problem, we use scalarisation of multi-objective optimisation, which exhibits flexibility in assigning different weights to a single objective—in the case of this study, either sum rate or fairness. Moreover, the problem is formulated with a penalty function to prevent optimisation violating the constraints of the optimisation function. Simulation results show that the proposed model outperformed the conventional approach by at least 17% in terms of throughput maximisation and fairness rate.
Cross-Laminated Timber Floor: Analysis of the Acoustic Properties and Radiation Efficiency
Authors: Granzotto Nicola , Marzi Arianna , Gasparella Andrea
Published: March 2022

Cross-Laminated Timber (CLT) is a building technology that is becoming increasingly popular due to its sustainable and eco-friendly nature, as well as its availability. Nevertheless, CLT presents some challenges, especially in terms of impact noise and airborne sound insulation. For this reason, many studies focus on the vibro-acoustic behavior of CLT building elements, to understand their performance, advantages and limitations. In this paper, a 200 mm CLT floor has been characterized in the laboratory, according to ISO standards, by three noise sources: dodecahedron, standard tapping machine and rubber ball. In order to understand the vibro-acoustic behavior of the CLT floor, measurements through the analysis of sound pressure levels and velocity levels, measured by dedicated sensors, were performed. Analysis was carried out in order to understand what is prescribed by the prediction methods available in the literature and by the simulation software. Then, a specific prediction law for the CLT floor under investigation was derived. Finally, an analysis on sound radiation index is provided to complete the vibro-acoustic study.
Evaluating Classical Airplane Boarding Methods for Passenger Health during Normal Times
Authors: Delcea Camelia , Milne R. John , Cotfas Liviu-Adrian
Published: March 2022

The COVID-19 pandemic has produced changes in the entire aviation industry, including adjustments by airlines to keep the middle seats of airplanes empty to reduce the risk of disease spread. In this context, the scientific literature has introduced new metrics related to passengers’ health when comparing airplane boarding methods in addition to the previous objective of minimizing boarding time. As the pandemic concludes and the aviation industry returns to the pre-pandemic situation, we leverage what we learned during the pandemic to reduce the health risk to passengers when they are not social distancing. In this paper, we examine the performance of classical airplane boarding methods in normal times but while considering the health metrics established during the pandemic and new metrics related to passenger health in the absence of social distancing. In addition to being helpful in normal times, the analysis may be particularly helpful in situations when people think everything is normal but an epidemic has begun prior to being acknowledged by the medical scientific community. The reverse pyramid boarding method provides favorable values for most health metrics in this context while also minimizing the time to complete boarding of the airplane.