bluuebunny/crossref_metadata_2025 huggingface dataset:
Measuring value in health care: lessons from accountable care organizations
Authors: Bao Chenzhang , Bardhan Indranil R
Published: March 2024

Accountable care organizations (ACOs) were created to promote health care value by improving health outcomes while curbing health care expenditures. Although a decade has passed, the value of care delivered by ACOs is yet to be fully understood. We proposed a novel measure of health care value using data envelopment analysis and examined its association with ACO organizational characteristics and social determinants of health (SDOH). We observed that the value of care delivered by ACOs stagnated in recent years, which may be partially attributed to challenges in care continuity and coordination across providers. ACOs that were solely led by physicians and included more participating entities exhibited lower value, highlighting the role of coordination across ACO networks. Furthermore, SDOH factors, such as economic well-being, healthy food consumption, and access to health resources, were significant predictors of ACO value. Our findings suggest a “skinny in scale, broad in scope” approach for ACOs to improve the value of care. Health care policy should also incentivize ACOs to work with local communities and enhance care coordination of vulnerable patient populations across siloed and disparate care delivery systems.
Health literacy and its association with mental and spiritual well-being among women experiencing homelessness
Authors: Rosenblad Andreas Karlsson , Klarare Anna , Rapaport Penny , Mattsson Elisabet , Gaber Sophie Nadia ,   in collaboration with the Women’s Advisory Board for Inclusion Health
Published: March 2024

Low health literacy (HL) has been linked to low self-rated health, reduced efficacy of behaviour change, and challenges in preventing, treating, or managing health conditions. People experiencing homelessness are at risk of poor HL; however, few studies have investigated HL in relation to mental and spiritual well-being among people experiencing homelessness in general, or women experiencing homelessness specifically. This cross-sectional study of 46 women experiencing homelessness in Stockholm, Sweden, recruited during the period October 2019–December 2020, aimed to examine how HL was associated with mental and spiritual well-being among women experiencing homelessness. Participants answered questions about socio-demographic characteristics (age, length of homelessness, education) and digital technology (mobile phone/the Internet) use, in addition to Swedish language versions of three questionnaires administered through structured, face-to-face interviews: the Communicative and Critical Health Literacy Scale, the General Health Questionnaire 12 and the Functional Assessment of Chronic Illness Therapy-Spiritual Well-Being. Data were analysed using linear regression, which revealed statistically significant associations between HL and mental well-being (p = .009), and between HL and spiritual well-being (p = .022). However, neither socio-demographic characteristics nor digital technology use were significantly associated with HL. In conclusion, promoting HL may improve mental and spiritual well-being in this vulnerable population. An advisory board of women with lived experiences of homelessness (n = 5) supported the interpretation of the findings and emphasised the need to consider HL in relation to basic needs such as ‘housing first’. Moreover, health information and services should be accessible to people with different degrees of HL.
‘The sanctuary of them all’: the politics of manpower and nationality in the armies in exile in the United Kingdom, 1940–4
Authors: Bud G C P
Published: March 2024

Historians have long remarked upon the political significance of the Allied armies from Continental Europe which were established on British soil during the Second World War. Much less attention has been devoted to the implications of maintaining and expanding these forces amid the very particular circumstances of wartime exile. This article examines three aspects of the tension between the imperatives to secure as many men as possible for military service while maintaining the distinctly ‘national’ character of the force as a whole. The failure to mobilize emigrants and expatriates is examined in the first section, while the second section shows how soldiers could use national identities to their own advantage and pass between foreign armies. The final section examines the far-reaching political consequences of the often-obscured ethnic diversity within the ranks, focusing particularly on Jewish soldiers.
Economy-environment-energy performance evaluation of power to hydrogen and power to methane considering renewable energy generation and carbon capture
Authors: Qiao Ning , Zhang Chao , Chen Haidong , Li Qiang , Zhang Jisheng , Tian Hongjie , Jing Hua
Published: March 2024

The integration of multienergy complementary systems has become increasingly prevalent to mitigate the waste of renewable energy. As power-to-gas (P2G) technology advances, converting electricity into gas presents a viable solution for bolstering the reliability of power supplies within integrated energy frameworks. P2G plays a pivotal role in harnessing unused renewable resources, such as wind and solar energy, to produce sustainable power. While numerous studies have explored the advancement of P2G, there remains a lack of comprehensive evaluation of its various component subsystems. This research aims to design a renewable energy power system, augmented by gas-fired generation, assessing the collective advantages of both the power-to-hydrogen (P-H) and power-to-methane (P-M) conversion processes within the P2G infrastructure. It is important to note that the economic potential of P-H conversion is currently constrained by cost factors within existing P2G systems. This study takes into account the dynamics of carbon trading, as well as the hydrogen and methane markets, to compare the economic, environmental and energy-specific benefits of P-H and P-M conversions under different power demand scenarios. The goal is to formulate enhanced strategies to optimize P2G deployment in these varied contexts.
Emotional Blunting in Depression in the PREDDICT Clinical Trial: Inflammation-Stratified Augmentation of Vortioxetine With Celecoxib
Authors: Sampson Emma , Kavakbasi Erhan , Mills Natalie T , Hori Hikaru , Schubert K Oliver , Fourrier Célia , Baune Bernhard T
Published: March 2024

Background

Emotional symptoms are recognized as a key feature in individuals with major depressive disorder. Previously, emotional blunting has been described both as a side effect of antidepressant treatment and as a symptom of depression. Little is known about the change of emotional blunting during antidepressant treatment.

Methods

The PREDDICT trial is a randomized, placebo-controlled, 6-week trial on the augmentation of vortioxetine with the anti-inflammatory agent celecoxib or placebo. Presently we report on exploratory secondary outcomes of changes in emotional blunting in depression assessed with the Oxford Depression Questionnaire (ODQ) total score and subscores from baseline to 8-week, 3-month, and 6-month follow-up assessments.

Results

In the whole group, there was a significant improvement in the ODQ total score and all subscores after 8 weeks. After stratification of participants into the treatment groups, the ODQ total score as well as subscores related to emotional blunting as a symptom of depression (reduction in positive emotions, not caring) improved between baseline and all follow-up time points in both treatment groups. Changes in subscores considered as a side effect of antidepressants (general reduction in emotions, emotional detachment) were inconclusive in both treatment groups. Overall, the placebo-augmented group showed slightly better results in changes of emotional blunting scores than the celecoxib group as did those with elevated inflammation at screening, regardless of treatment group.

Conclusions

This analysis suggests favorable effects of vortioxetine on emotional blunting in both short- and long-term course. The beneficial impact of vortioxetine on emotional blunting was weaker in celecoxib-augmented patients compared with placebo, possibly due to pharmacokinetic interactions.

Clinical Trials Registration: Australian New Zealand Clinical Trials Registry (ANZCTR), ACTRN12617000527369p. Registered on 11 April 2017, http://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12617000527369p.
Advancing Local US Transitional Justice Initiatives: A University Partnership Alongside Descendant Communities
Authors: Mann Linda J
Published: March 2024

This article suggests that the ongoing denial by the USA federal government of historical human rights infractions against African Americans and people of African descent led to a proliferation of local transitional justice efforts. Drawing from a university-driven transitional justice project, this article offers an analysis of local initiatives and highlights one of its six transitional justice collaborations. The university project sought to decolonize its structure and approach, drawing on methods of cultural humility, desire-based research and deep listening. This article responds to the success and challenges of decolonizing a transitional justice project within academia. Findings suggested that local initiatives face obstacles due to enduring legacies of harms and silenced histories, necessitating communities to substantiate their claims. Capacity issues and funding are challenged due to centuries of disinvestment. The article offers lessons learned for potential ally organizations who seek to advance transitional justice where government-sponsored redress continues to be denied.
Acute malaria suppresses the B lymphocytic niche in the bone marrow through the alteration of CXCL12-abundant reticular cells
Authors: Lee Michelle Sue Jann , Matsuo-Dapaah Julia , Del Rosario Zorrilla Camila , Omatsu Yoshiki , Nagasawa Takashi , Uemura Shun , Iwama Atsushi , Ishii Ken J , Coban Cevayir
Published: March 2024

Bone marrow is a dynamic organ composed of stem cells that constantly receive signals from stromal cells and other hematopoietic cells in the niches of the bone marrow to maintain hematopoiesis and generate immune cells. Perturbation of the bone marrow microenvironment by infection and inflammation affects hematopoiesis and may affect immune cell development. Little is known about the effect of malaria on the bone marrow stromal cells that govern the hematopoietic stem cell (HSC) niche. In this study, we demonstrate that the mesenchymal stromal CXCL12-abundant reticular (CAR) cell population is reduced during acute malaria infection. The reduction of CXCL12 and interleukin-7 signals in the bone marrow impairs the lymphopoietic niche, leading to the depletion of common lymphoid progenitors, B cell progenitors, and mature B cells, including plasma cells in the bone marrow. We found that interferon-γ (IFNγ) is responsible for the upregulation of Sca1 on CAR cells, yet the decline in CAR cell and B cell populations in the bone marrow is IFNγ-independent. In contrast to the decline in B cell populations, HSCs and multipotent progenitors increased with the expansion of myelopoiesis and erythropoiesis, indicating a bias in the differentiation of multipotent progenitors during malaria infection. These findings suggest that malaria may affect host immunity by modulating the bone marrow niche.
The Metameric Echinoderm
Authors: Turner R L
Published: March 2024

Synopsis

Animal phyla are distinguished by their body plans, the ways in which their bodies are organized. A distinction is made, for example, among phyla with bodies of many segments (metameric; e.g., annelids, arthropods, and chordates), others with completely unsegmented bodies (americ; e.g., flatworms and mollusks), and a few phyla with bodies of 2 or 3 regions (oligomeric; e.g., echinoderms and hemichordates). The conventional view of echinoderms as oligomeric coelomates adequately considers early development, but it fails to recognize the metameric body plan that develops in the juvenile rudiment and progresses during indeterminate adult growth. As in the 3 phyla traditionally viewed to be metameric (annelids, arthropods, and chordates), metamery, or metamerism, in echinoderms occurs by (1) subterminal budding of (2) serially repeated components of (3) mesodermal origin. A major difference in most echinoderms is that metamery is expressed along multiple body axes, usually 5. The view of a metameric echinoderm might invite new discussions of metazoan body plans and new approaches to the study of morphogenesis, particularly in comparative treatments with annelids, arthropods, and chordates.
121 Unraveling the biology of bovine conceptus elongation
Authors: Lamberti Gabriela , Goldkamp Anna K , Lopes Maria , Bonmann Nicole , Van Rhijn Samuel , Hagen Darren E , Moraes Joao
Published: March 2024

The establishment of pregnancy is a well-regulated process that involves the development of a viable blastocyst, conceptus elongation, maternal recognition of pregnancy, implantation, and the onset of placentation. Pregnancy loss is a major factor limiting reproductive efficiency in livestock species. In cattle, most embryonic losses occur during the first month of gestation. The genetic and epigenetic regulation of embryonic and placental development in ruminants is still poorly understood. To investigate the transcriptome profile of the bovine conceptus during elongation, Angus heifers (n = 8) were superovulated and bred using semen from a proven high-fertility Bos indicus sire. Heifers were flushed on d 14 of gestation using a standard nonsurgical flushing technique. Recovered conceptuses were individually photographed and snap-frozen for total RNA extraction and bulk mRNA sequencing. Recovered conceptuses were classified based on size into ovoid (0.2 – 5mm; n = 6), tubular (<15 mm; n = 7), or filamentous (>16 mm; n = 7). Differentially expressed genes (DEG: FDR < 0.05) were determined using DESeq2 analysis and were functionally annotated using DAVID. The most pronounced transcriptomic changes were identified between the ovoid and tubular stages, with 5,937 DEGs (2,913 upregulated and 3,024 downregulated) and affected pathways were primarily involved in ribosome assembly and rRNA processing. There were 3,966 DEGs (2,069 upregulated and 1,897 downregulated) between the ovoid and filamentous stages, and the most significant pathways were involved in protein translation, embryonic development, and cell cycle. The transition between tubular to filamentous presented 1,435 DEGs (775 upregulated and 660 downregulated) and significant pathways were involved in protein degradation, cell division, cell cycle, protein stabilization, and mRNA splicing. Our findings indicate that the greatest alterations in the transcriptome occur precisely at the initiation of elongation, suggesting that the bovine conceptus undergoes major structural changes at the outset of this process. This major shift in gene expression is presumed to be necessary to support the subsequent exponential growth phase of elongation, required for maternal recognition of pregnancy.
42 Challenges for agricultural social media influencers engagement with young, non-ag adults
Authors: Bennett Samantha P , Martin David , Sawyer Jason T , Rodning Soren P , Mulvaney Don R
Published: March 2024

Segments of society question the welfare of animals in production, whether types of product consumption have a negative impact on personal health, and environmental sustainability of producing livestock. As most Americans are three or more generations removed from production agriculture (American Farm Bureau, 2020), they have minimal incentive to question unsubstantiated information purported by anti-animal agriculture groups. Ag communication strategies often seem to fall short compared with anti-agricultural messages shared throughout the media infosphere. Prominent forms of digital / social media (SM) platforms have infiltrated daily lives of society. With the significance of engagement factors such as likes, comments, shares, and saves on such platforms in mind, our research sought to assess the willingness of SM users to interact with content on Instagram created by agricultural social media influencers (SMIs). Participants (n = 855; ages 18-30 from the U.S. and a non-ag background) were recruited through Prolific and anonymously completed a Qualtrics survey. Quantitative data were responses to questions about willingness to engage with both general content and agriculture-related content. Participants were shown images and narrative content from well-established agricultural SMIs. Participants were surveyed both before and after being shown example image posts of agricultural SMIs. Data were analyzed utilizing SPSS with independent variables consisting of general content versus agricultural content while dependent variables were comprised of the summation of participant engagement type willingness which was operationalized by numerically converting the five-point Likert scale. Results showed differences (P < 0.05) in willingness to engage with agricultural SMI through likes, comments, shares, and saves than with general content. Results imply that agricultural SMIs will have more limited opportunities to facilitate engagement with young adult Instagram users than those that share generalized content. These outcomes reinforce a communication dilemma around SM engagement that focuses on reaching non-ag audiences with image and evidence-based agricultural content.
41 Social and institutional trust: A comparison between non-associated and associated producers in Southeast Brazil
Authors: Reis Beatriz , Casado Adrieli , Bernardes Elaine , Miasaku Celso , McLain DeAnja A , France Otavio , Sarturi Jhones O
Published: March 2024

The social and institutional trust between two groups of dairy producers in the State of Sao Paulo, Brazil was evaluated. The entity used in this comparison was the Rural Producers Association (RPA), in which two groups were formed: a) RPA members (AG); and b) RPA non-members (NAG). The questionnaire developed used model questions from the World Values Survey. Among a total of 38 producer-members of the RPA, 22 (58%) responded to the questionnaire. For the non-member producers, 44 were identified, while 39 (89%) responded to the questionnaire. To assess social trust, the following question was asked: "Generally speaking, would you say that people can be trusted or that we need to be very careful when dealing with people?". In AG, 81.8% of the producers responded that we need to be very careful when dealing with other people, and among the NAG producers, 87.2% also agreed with such a statement. Despite the lack of social trust in AG, 77.1% of the members fully or partially trust their association. To assess levels of trust in national institutions by producers, a question was asked as follows: "To what extent do you trust the institutions listed below?". For more than 50% of the NAG group, none of the institutions presented deserved full trust, reinforcing the lack of trust, as indicated in the previous question. For those in the NAG group that chose the option "I trust completely", the institutions opted were as follows: religious (46.2%), research institutions (43.6%), public universities (38.5%), and rural extension services (33.3%); while those opted as “I do not trust" were represented by political parties (82.1%), the municipal government (64.1%), and the national congress (61.5%). In the AG group, the institutions with the greatest levels of trust were the rural extension services (54.5%) and the RPA (54.5%), religious institutions (40.9%), public universities (40.9%), and research institutes 36.4%. Moreover, the institutions least trusted were political parties (63.6%), national congress (63.6%), municipal government (36.4%), and land organizations (36.4%). None of the producer groups were officially affiliated with political parties. An open-ended question was asked as follows: "What do you think about the current situation of the association, cooperative, or dairy in which you participate or commercialize milk with?" In the AG, 81.75% see RPA positively, and 18.25% believe that the association was in a bad situation and could even end. In the NAG, 59% of the producers commercialize milk in some type of organization (other than the RPA); in which among these, 43.47% have a negative image of such an organization in general. It is suggestive that regardless of the RPA participation, producers interviewed lack social trust. Future analysis is warranted to involve a multi-location and a broader population sampling.
34 A survey of the Oklahoma State University Cowculator user preferences
Authors: Talley Samuel R , Beck Paul A , Gross Megan A , Kingsley Scarlett , Lalman David L
Published: March 2024

The Oklahoma State University Cowculator is a free, easy to use model designed to assist cattle producers in making informed decisions regarding the nutrition of their herd. The Cowculator operates as a spreadsheet-based model, which collects information from the 2016 National Academy of Science, Engineering, and Medicine’s Nutrient Requirements for Beef Cattle, previous editions of the book, and previous research data. Producers have the option to adjust animal characteristics, feed and forage values, and diet composition to fit certain production objectives. In 2022, a Qualtrics survey was developed to determine perception of users of the program and their likes and dislikes. When users download the program, they are required to enter their name, email address, and zip code. A link to the survey was emailed to each address registered within the Cowculator database, with an email reminder sent out 30 days later. The survey yielded 233 responses. According to user download data, in 2022 the Cowculator was downloaded 2,090 times from 41 states and 53 countries. Based on email addresses ending in “.edu” the program was downloaded by faculty or students from 135 different universities, colleges, or other technical institutions. Of the respondents, 60% identified as beef cattle producers, 16% as industry professionals (e.g., consultants, veterinarians), 11% as extension personnel, 7% as research or teaching faculty, and 6% as students. Regarding the type of enterprise which the program was used for, 52% responded with cow/calf, 33% growing or stocker cattle, and 15% finishing cattle. The survey indicated that 88% of respondents were able to utilize the program effectively to evaluate or modify their nutrition program, while 16% indicated they were not successful in using the program. The survey asked respondents to give their opinion on the accuracy of the program. 69% indicated that it yielded “mostly accurate” projections, 23% responded with “very accurate”, and 5% perceived the program to be inaccurate or marginally accurate. Users of the program estimated the average annual benefit of the program to be $1,500. The respondents were asked to select what they liked about the program and what they felt needed improvement. It was indicated that the most liked features were that the program was free and that it was easy to use. The features that were indicated as needing improvement where the limited pre-added list of feedstuffs and the lack of availability of a simplified app version of the program. The results of this survey provide valuable insights regarding user demographics, as well as what can further be adjusted within the Cowculator to best fit the needs of the users.
92 Rearing system influenced the performance, GI-parasite burden, and immune profile of pregnant does during winter
Authors: Lamsal Sadikshya , Karki Uma , Chaudhary Santoshi , Dhakal Durga P , Paneru Bidur
Published: March 2024

Small ruminants in the Southeast are predominantly raised outdoors, regardless of seasons and associated weather conditions. Severe weather in winter can decline the performance of pregnant animals and compromise their performance and immunological states. Indoor raising of pregnant animals during winter may minimize the adverse effect of cold stress; however, such potential has not been evaluated. This study hypothesized that performance, GI-parasite burden, and immune response would differ between pregnant does raised outdoor and indoor during winter. The objective of this study was to evaluate the performance, GI-parasite burden, and hematological, biochemical, and immunological parameters of pregnant does raised outdoor and indoor in winter. The study was conducted from late January to early April 2023. Sixteen pregnant Kiko does were divided into outdoor and indoor groups. Outdoor group was kept in one grazing plot consisting of mobile shelters, mineral feeders, hay feeders, and watering troughs. Indoor group was kept in individual pens consisting of containers for water, minerals, hay, and grain. Both groups were given corn and soybean mixed in 3:2 ratio (0.8% of animal body weight), ad libitum hay, minerals, and water. Daily ambient temperature and relative humidity (RH) data were collected for both sites, and temperature-humidity index (THI) was calculated. Animal performance data [body weight, body condition score (BCS), and FAMACHA score] and fecal samples were collected on d 1, weekly during the study, and at the end of the study. Fecal samples were analyzed for the type and quantity of GI parasites using the McMaster technique, and parasite eggs per gram of animal feces (EPG) was calculated. Blood samples were collected on d 1, 34, and 69 and analyzed for hematological, biochemical, and immunological parameters. Data on weather, animal performance, and hematological, biochemical, and immunological parameters were analyzed using the GLM procedure and EPG data were analyzed using the Wilcoxon Rank-Sum test in SAS 9.4. Indoor temperature was higher (7%, P < 0.01), RH was lower (8%, P < 0.05), and THI was higher (5%, P < 0.01) vs. outdoor. Overall, indoor does had better FAMACHA by 9% (P < 0.05) and greater EPG by 35% (P < 0.05). Eosinophils were reduced by 73% (P < 0.05), calcium was increased by 11% (P < 0.01), and total bilirubin was greater by 86% (P < 0.05) in indoor does at the end of the study (P < 0.05) vs. outdoor does. Results showed that performance, GI-parasite burden, and biochemical and immunological parameters of pregnant does were influenced by the rearing system during winter.
88 Impacts of trace mineral source and ancillary drench on steer performance during a 60-day backgrounding phase
Authors: McKnight Madeline , Harvey Kelsey M , Karisch Brandi B , Cordero Juan C , Drewery Mason , Durst Libby S , Colombo Eduardo , Cooke Reinaldo F , Russell Jason R
Published: March 2024

This experiment evaluated the impacts of supplementing organic complexed Cu, Co, Mn, and Zn on productive and health responses of high-risk beef cattle during a 60-d backgrounding phase. Crossbred steers (n = 120) were purchased at auction and transported to the experimental facility. Steer BW was recorded at arrival (d -1; initial shrunk BW = 227.7 ± 1.3 kg). On d 0, steers were ranked by BW and allocated to 1 of 8 groups (8 steers/group) and housed in drylot pens (6 m x 12 m) equipped with GrowSafe automated feeding systems (Model 8000; 2 bunks/pen; d 0 to 60). Groups were randomly assigned to receive a total mixed ration (TMR; 47% corn) containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 40); 2) organic complexed sources of Cu, Mn, Co, and Zn (AAC; Zinpro Availa 4; Zinpro Corp., Eden Prairie, MN; n = 40); or 3) AAC and an organic complexed trace mineral drench (APF; 30 mL/hd; Zinpro ProFusion, Zinpro Corp.) on d 0 and ancillary to morbidity treatment (n = 40). Diets provided the same daily amount of energy, protein, macro minerals and trace minerals based on 7 g/steer daily of Zinpro Availa 4. Steers were assessed for bovine respiratory disease (BRD) signs daily. Liver biopsies were performed on day 0, 28 and 60. Final BW was recorded on d 60 and 61. Blood samples were collected on d 0, 2, 6, 10, 13, 21, 28 and 45. No treatment differences were detected (P ≥ 0.23) for TMR intake, final BW, ADG, feed efficiency, or BRD incidence. Mean liver Co concentrations were greater (P = 0.02) in AAC and APF compared with INR steers. Mean liver Cu was greater (P = 0.02) in APF compared with AAC steers. Liver Zn tended to be greater (P = 0.10) on d 28 but less (P = 0.05) on d 60 for INR compared with AAC and APF steers. Plasma cortisol was least (P = 0.05) for steers supplemented with AAC on d 6, whereas AAC steers tended to have greater (P = 0.09) plasma cortisol on d 13 compared with APF. Plasma haptoglobin tended to be greater (P ≤ 0.10) for INR steers on d 28 and 45 compared with AAC and APF. While supplementing cattle with AAC or INR results in similar animal performance and clinical disease, AAC and APF reduces stress and acute phase protein responses.
85 Evaluation of a Saccharomyces cerevisiae fermentation product on performance, plasma metabolites, rumen parameters, and fecal pathogen shedding in feedlot steers
Authors: Gong Wendy , Zhang Guolong , Foote Andrew P , Wilson Blake K
Published: March 2024

The objective of the experiment was to evaluate a commercial Saccharomyces cerevisiae fermentation product (SCFP; NaturSafe, Dimond V, Cedar Rapids, IA) on performance, plasma metabolites, rumen parameters, and fecal pathogen shedding in feedlot steers. Crossbred steers (n = 61; 271 ± 48.1 kg) were sourced from 3 different research units at Oklahoma State University and assigned to 1 of 3 experimental treatments in a randomized complete block design. Steers were group housed in pens equipped with an automated individual feed intake system. Treatments consisted of a negative control (CON), CON with tylosin phosphate (TYL; 9.9 mg · kg-1 of diet DM), or CON with SCFP (1.63 g · kg-1 of diet DM). Body weight and feed efficiency did not differ among treatments (P ≥ 0.12). However, steers fed SCFP tended to have greater ADG from d 35 to 56 (P = 0.10). In addition, steers fed SCFP exhibited increased DMI from d 56 to 112 (P = 0.03), a tendency for increased DMI from d 112 to final (P = 0.07), and increased DMI overall (P = 0.05). There was no treatment × time interaction for pH, pH change, or lactate concentrations in rumen fluid (P ≥ 0.11), but all were impacted by time (P < 0.01). A treatment × time interaction (P < 0.01) was detected for fecal pH and fecal pH change, with the pH being greater in TYL fed steers on d 56 and in CON fed steers on the final day. Steers that consumed CON had a reduced fecal pH (P < 0.01) and steers consuming SCFP tended to have a reduced fecal pH (P = 0.08) from d 35 to 56 compared with TYL fed steers, while both CON and SCFP fed steers experienced an increase in fecal pH from d 56 to 112 (P < 0.01). A time effect was detected (P ≤ 0.04) for plasma glucose, lactate, urea nitrogen, and non-esterified fatty acid concentrations among treatment groups. A treatment × time interaction was observed (P < 0.02) for stx2 genes of Shiga toxin producing Escherichia coli (STEC) in individual fecal samples where SCFP and TYL fed steers tended to exhibit an increased concentration compared with CON fed steers on d 14, while on d 190 CON and SCFP fed steers exhibited an increased concentration compared with TYL fed steers. Overall, STEC, E. coli O157:H7, and Salmonella concentrations were all impacted by time (P < 0.001). This experiment suggests that SCFP could be utilized as a potential alternative to TYL in feedlot cattle without adversely affecting cattle performance. However, SCFP did not impact pathogen shedding. In addition, CON fed steers performed similarly to both SCFP and TYL fed steers.
75 AI-Enhanced Hematocrit Analysis: Revolutionizing rapid anemia detection in small ruminants
Authors: Dargan Seymone , Siddique Aftab , Pech-Cervantes Andres , van Wyk Jan , Panda Sudhanshu , Mahapatra Ajit , Morgan Eric , Appleberry Ebonie , Carter India , Lewis Savana , Terrill Thomas H
Published: March 2024

In recent times, there has been a growing focus on the well-being and healthcare of small ruminants, particularly in relation to the issue of anemia due to infection with blood-feeding gastrointestinal nematodes, such as Haemonchus contortus. The objective of this study was to assess the hematocrit levels in blood samples obtained from small ruminants, specifically goats. Additionally, the study is an attempt to design and create a quick sensor for identifying anemia, which could be conveniently used on farms. A total of 75 mature, intact male Spanish goats were subjected to hematocrit analysis to ascertain their spectrum of hematocrit values and association with anemic conditions. Simultaneously, a unique sensor with user-friendly features was developed to promptly provide farmers with feedback on the anemic status of animals using AI-based machine learning algorithms, enabling timely intervention. The sensor utilized a semi-invasive approach with minimal blood sample requirement. 30 µL of blood was dropped on glycerol soaked Whatman filter paper No. 1, and images were taken at 90 sec, 150 sec, and 270 sec to capture the blood pattern on filter paper soaked in glycerol using a cell phone. These images were correlated with actual hematocrit value obtained from conventional hematocrit analysis. A RMSprop with adjusted learning rate convolution neural network (RMSprop-CNN) based images classification model was developed for the classification of different patterns at different levels of hematocrit. The model was trained on a total of 1,000 images with a training and testing split of 80:20 ratio. For the optimization process, the Adam optimizer with a learning rate of 0.001 is employed. The model is compiled with a categorical cross-entropy loss function, aiming to improve its accuracy metric over training iterations. The initial studies demonstrated a detection accuracy level of 70.31 % at 10 epochs in identifying different hematocrit levels (Level 1; healthy, to Level 5; severely anemic) but improved significantly up to 94.89 % by 100 epochs, resulting in a notable reduction in the time and level of knowledge previously necessary for conducting such evaluations. The present study not only provides valuable insights into the hematological characteristics of small ruminants, but also establishes a foundation for the development of a straightforward and efficient method for detecting anemia. Consequently, this advancement has the potential to enhance the overall care and well-being of animals within agricultural environments.
80 Effect of copper bolus administration on circulating copper concentration, blood metabolites and parasite load
Authors: Harmonson Tyler K , Cassens Andrew M , Owsley Walter F , Runyan Cheyenne L , Webb Edward , Wellmann Kimberly B
Published: March 2024

In Texas and Southeastern United States Cu concentration in the soil is in the lower 50th percentile, this leads low bioavailability of copper for livestock. Therefore, a 70-d completely randomized block design study was conducted to determine the effects of Cu and fecal egg count in grazing beef cows (n = 40). Cows were blocked by parity and body weight (BW) then randomly assigned to 1 of 2 treatments: standard offering of copper via free choice mineral (CON; Ragland Sweet Mag Mineral Mix, 425 ppm Cu) and those receiving a 25 mg Cu rumen bolus in addition to the standard offering (Santa Cruz Animal Health, Dallas, TX). Cattle were managed as a single herd at the with equal access to water, a grain supplement, and a warm season grass pasture. Fecal samples via rectal grab, blood via coccygeal venipuncture, BW, BCS, hair coat (1 being the healthiest) and shedding (1 being the most slick) scores were all collected on d 0, 10, 24, 38, and 66 relative to treatment initiation. Sera were analyzed to determine circulating Cu, macrominerals, urea, creatinine, and various blood proteins. Fecal egg counts were conducted to determine Coccidial, Moniezia, and Trichostrongyle egg content. Data were analyzed using the GLIMMIX procedure of SAS 9.4 (SAS inst. Inc, Cary, NC). No treatment × day interaction (P = 0.988) was observed for circulating Cu; however, Cu decreased (P < 0.001) in all cattle over time, and TRT tended (P = 0.090) to have increased Cu compared with CON. No interactions or main effects occurred (P > 0.509) for BW. No interaction (P = 0.4389) occurred for BCS, hair, or shedding scores. Hair scores (P < 0.001), shedding scores (P < 0.041), and BCS(P < 0.001) decreased in all cattle over time. Body condition for TRT (5.2 ± 0.056) was greater (P =.0024) than CON (4.9 ± 0.057). Hair score by day decreased (P < 0.001) in TRT, while the shedding had a tendency of increasing (P = 0.0411) by TRT. There were no interactions or main effects on Coccidial (P < 0.220) or Trichostrongyle (P < 0.274) egg content. While no interaction (P = 0.673) for Moniezia was observed, TRT tended (P = 0.095) to have fewer Moniezia eggsthan CON. No interactions (P > 0.100) were observed for any of the blood metabolites, and while they differed (P < 0.05) day to day for all cows, no treatment effects occurred (P > 0.10). Although Cu bolus administration at this concentration did tend to change Cu copper in the animal, effects resulting from it were minimal.
82 Effects of a red-clover containing mineral on heifer behavior and fertility
Authors: Purvis Tyler A , Anderson Leslie H , Weinert-Nelson Jennifer R , Vanzant Eric , Hamilton Tracy A , Miller Dan , Johns John , Davis Brittany E
Published: March 2024

Heat stress reduces reproductive efficiency in beef cattle. Recent evidence suggests that supplementation of red clover can alleviate the physiological impacts of fescue toxicosis which could decrease heat stress. Our objective was to determine the effect of supplementing heifers grazing endophyte-infected tall fescue (E+ TF) with a conventional mineral containing red-clover leaf. We hypothesized that heifers supplemented with the red-clover leaf mineral would have greater conception rates to AI (CRAI) in times of possible heat stress compared with heifers supplemented with conventional mineral. A grazing study was conducted from May – August in 2021, 2022, and 2023. Yearling beef heifers (n = 32, 36, 40 heifers yr-1, respectively) were blocked by body weight, hair coat score and color into 4 grazing blocks that were assigned to 1 of 2 treatments (2 blocks treatment-1): 1. conventional non-medicated loose mineral control (0%-RC), or 2. conventional loose mineral + 20% wt/wt red clover leaf (20%-RC). Mineral treatments were provided in ground, 3-compartment mineral feeders ad libitum. Each grazing block was assigned to four, 0.8- ha. pastures consisting of predominantly E+ TF and were rotationally managed over the course of the study. Each year estrus was synchronized suitable for fixed time AI in May and all heifers resynchronized in June. In addition, each heifer was fitted with an ear-tag sensor (CowManager SensOor, Agis Automatisering BV, Harmelen, the Netherlands) for continuous behavioral monitoring (including high activity and activity). The CRAI were analyzed with the GLIMMIX procedure of SAS using the binomial distribution link logit function (v.9.4., SAS Inst. Inc., Cary, NC). Total daily activity and high activity reported by CowManger during May and June were analyzed by the MIXED procedure with repeated measures of SAS. The CRAI were similar for 20%-RC (67.30 ± 9.80%) and 0%-RC (60.70 ± 9.80%) heifers following the first service of AI (P = 0.51) during a thermoneutral period in May. In contrast, CRAI was greater (P = 0.02) for 20%-RC heifers (48.67 ± 13.47%) than CM (15.10 ± 13.47%) in June when temperatures increased to potential heat stress levels. Overall, conception rate after two rounds of AI for 20%-RC (78.30 ± 9.30%) heifers tended to be greater (P = 0.08) than CM (62.20 ± 9.30 %). While high activity (associated with estrous behavior) reported by CowManager did not differ between treatments (20%-RC: 203.94; 0%-RC: 213.90; SEM: 8.65 min d-1; P = 0.25), normal daily activity of 20%-RC heifers (150.69 ± 10.40 min d-1) tended to be greater (P = 0.06) than CM (126.33 ± 8.65 min d-1). Results of this study suggest supplementing red-clover containing mineral may mitigate negative effects of consuming E+ TF including decreased conception rates during times of heat stress.
94 Smartphone integration in anemia detection for small ruminants with Convolutional Neural Networks
Authors: Randall Reginald J , Pech-Cervantes Andres , van Wyk Jan , Panda Sudhanshu , Mahapatra Ajit , Morgan Eric , Stegall Kensley , Brown Davia , Manns Ethan , Saunders Shaylon , villa Andrea Rubio , Plummer Aniyah , Terrill Thomas H
Published: March 2024

Anemia, often caused by internal parasites like Haemonchus contortus, presents significant health and productivity challenges for small ruminants. The primary goal of this study was to accurately distinguish between healthy and anemic goats using an image classification system focused on eye conjunctiva images. Over 5,000 eye conjunctiva images from 75 goats were collected at Fort Valley State University farms over a 3-mo period using smartphone cameras. These images were randomly divided into training (70%) and testing (30%) datasets, with each group containing five subfolders corresponding to FAMACHA scores of 1, 2, 3, 4, and 5. A Convolutional Neural Network (CNN) algorithm was utilized for image analysis, incorporating data augmentation techniques such as Resize, RandomHorizontalFlip, RandomVerticalFlip, and RandomRotation. The CNN model was built on the Google Colaboratory platform using CUDA 11.2 and the PyTorch machine learning framework, incorporating three ConvNet layers. The model training used the Adam Optimizer with a slower learning rate of 0.001 and a weight decay of 0.0001 to prevent exploding gradient issues, alongside ReLU and the cross-entropy loss function over 1000 epochs. Results demonstrate that the CNN model was highly effective in classifying eye conjunctiva images of goats to detect anemia based on FAMACHA scores.
101 Effects of moderate exercise regimen on reproductive development of replacement beef heifers reared in drylots at a high stocking density
Authors: Pickett Autumn , Cooke Reinaldo F , Rahmel Logan W , Mackey Shea , Drewery Mason , Cordero Juan , Mackey Teegan , Daigle Courtney L , Martins Thiago , Harvey Kelsey M
Published: March 2024

This experiment evaluated growth and reproductive development of beef heifers reared at a high stocking density and provided with access to an exercise area. The experiment was replicated over 2 yr, with a total of 180 Angus × Brahman heifers. Each year, 90 heifers were ranked by age (264 ± 1 d) and body weight (BW; 200 ± 2 kg) on d 0, and assigned to a) 1 of 6 drylot pens (10 × 14 m pens; 10 heifers/pen) resulting in a stocking density of 14 m2/heifer (DENS), or b) 1 of 3 paddocks (2-ha paddocks; 10 heifers/paddock) resulting in a stocking density of 2,000 m2/heifer (CON). On d 15, 3 DENS pens were randomly selected and given access to an exercise area (DENS-EX) for 1 h three times weekly. Negligible forage was available for CON heifers, whereas all heifers received the same limit fed diet which averaged 13 kg/heifer daily of a corn-based diet. Heifer shrunk BW was recorded after 16 h of feed and water withdrawal on d -3 and 225 for BW gain calculation. Heifer temperament (chute score, exit velocity, and temperament score) were evaluated on d 0 and 225. Heifers were also fitted on d 0 with a pedometer behind their shoulder and an ear tag (CowManager, Select Sires, Plain City, OH) to record behavioral responses. Each week, pedometer results were recorded, and blood samples were collected for puberty evaluation via plasma progesterone. Heifers from CON had more (P < 0.05) steps per week compared with DENS and DENS-EX (23,973, 6,706, and 12,354 steps/week, respectively; SEM = 1,247), and DENS-EX heifers had more (P < 0.05) steps per week compared with DENS. Heifers from CON spent more (P < 0.05) time ruminating and eating compared with DENS and DENS-EX. No treatment effects (P ≥ 0.91) were detected for heifer BW gain (0.820, 0.815, and 0.824 kg/d for CON, DENS, and DENS-EX, respectively; SEM = 0.016). Heifers from DENS had greater (P ≤ 0.05) exit velocity and temperament score on d 225 compared with DENS-EX and CON. Heifers from DENS experienced delayed puberty attainment (treatment x day; P < 0.01) compared with DENS-EX and CON. A greater (P ≤ 0.05) proportion of CON heifers were pubertal by day 105 compared with DENS and DENS-EX (30.0, 11.6, and 15.0%, respectively; SEM = 6.5), whereas a greater (P < 0.05) proportion of CON and DENS-EX heifers were pubertal by d 154 compared with DENS (61.7, 66.7, and 43.3%, respectively; SEM = 6.5). Results from this experiment demonstrate that providing heifers with an exercise area alleviated the negative effects associated with high stocking density on their reproductive development. This work is supported by the USDA-NIFA AFRI program (#2021-67015-34083).
5 Farm-level management practices in forage-based beef cattle systems and their influence on sustainability
Authors: Mullenix M Kim , Smith William B , Dillard Sandra L , Neto Jose D Pereira
Published: March 2024

Sustainability is a ‘buzzword’ often used within and outside of the agriculture industry. Southeastern beef cow-calf and stocker operations often utilize management practices which embody the core definition of sustainability to improve land resource, economic, and social viability. In forage-based production systems, year-round management practices are generally viewed by the scientific community in terms of production and economic responses. However, the continual global shift in emphasis on sustainable practices creates an opportunity to view these practices through a different lens. Goals of grazing-based beef systems often include optimizing the number of grazing days per year, reducing hay and supplemental feeding period duration, and strategic supplementation during grazed forage availability gaps. Methods for increasing the grazing season length per year may include stockpiling, inclusion of multi-species mixtures, increasing species diversity on farm, and optimizing stocking strategies. Outside of associative effects of feed supplementation are potential ancillary benefits of supplementation on the plant-animal-soil interface. As an example, feed-through fertilization influences forage growth production responses and stocking density modulation in grazed beef cattle systems. The role of mineral supplementation and ionophore inclusion in beef cattle diets is well documented from an animal performance perspective, but to a lesser extent on how using these practices may influence forage or soil response variables. Stacking management practices may elicit positive animal performance responses, while potentially reducing outside inputs into the system. The question then becomes if this is economically viable compared with a more traditional forage-based system. From a research perspective, systems-level studies are more prevalent than in the past, but funding models require scientists to evolve and lead with the sustainability message rather than from a production viewpoint. Valuing management practices from an ecosystem services view is a potential area of opportunity for researchers, industry and farmers to highlight strengths of forage-based beef cattle production systems from a consumer perspective.