bluuebunny/crossref_metadata_2025 huggingface dataset:
Thrombodynamic method of evaluation of some hemostatic parametersin healthy pregnant women and in women with preeclampsia
Authors: Kuznik Boris I. , Zagorodnyaya Emma D. , Iozefson Sergey A. , Isakova Natalia V.
Published: February 2019

Hypothesis/aims of study. During pregnancy, hypercoagulation develops and fibrinolysis is inhibited. These changes are particularly pronounced in pregnancy complicated by preeclampsia. The existing techniques to evaluate hemostasis are rather laborious and do not provide a complete information of the volume and density of the fibrin clot. We aimed to conduct comparative studies of the state of the hemostasis system by conventional test-tube and thrombodynamic methods in healthy non-pregnant women and in pregnant women who are suffering and are not suffering from preeclampsia.

Study design, materials, and methods. We examined 40 pregnant women aged 17–33 years (mean age 24.3 ± 3.2 years) at the gestational age of 35–39 weeks. In 18 women, pregnancy was complicated by preeclampsia. The control group consisted of 35 healthy women (mean age 25.1 ± 2.6 years). The thrombodynamic characteristics of the clot were evaluated using the T-2 Thrombodynamics Recorder device (GemaKor Ltd., Russia) using the following parameters: Tlag, clot growth delay; Vi, initial growth rate; Vs, steady growth rate; D, clot density; C30, the size of the main clot in the 30th minute.

Results. When evaluating the hemostasis system by the thrombodynamic method in healthy pregnant women and in women with preeclampsia compared with non-pregnant women, the onset time of clot formation was significantly reduced, the initial and steady rate of the clot appearance, as well as the clot density and the clot size being increased.

Conclusion. The method for evaluating thrombodynamic properties of the blood clot is objective and visual way to estimate hemostasis. It complements the coagulogram indices and gives a clear view of the hemostasis system.
APC-resistance as a possible predictor of recurrent thrombosis in women with Factor V Leiden
Authors: Nikolayeva Maria G. , Momot Andrey P. , Zaynulina Marina S. , Momot Ksenia A. , Yasafova Natalia N.
Published: February 2019

Hypothesis/aims of study. The current analysis was undertaken to elucidate the role of Factor Va resistance to proteolytic cleavage by activated protein C in FVL(1691)GA female carriers in the development of acute and recurrent thromboses.

Study design, materials and methods. A prospective clinical cohort study of 1100 women of reproductive age was conducted, with the course and outcomes of 2,707 pregnancies analyzed. Two cohorts were specified: the main group consisted of 500 patients with FV(1691)GA genotype, and the control group consisted of 600 patients with FVL(1691)GG genotype.

Results. FVL(1691)GA genotype was significantly associated with the development of venous thromboembolic complications (VTEC) compared to FVL(1691)GG genotype (OR 9.3; p < 0.0001). Episodes of recurrent thrombosis during and outside of pregnancy were registered only in FVL(1691)GA patients (OR 5.7, p = 0.2). In all cases, at the time of the thrombotic event and during the period before the episode of acute or recurrent thrombosis, an APC resistance normalized ratio (NR) value was ≤ 0.49, with no episodes of VTEC registered with an APC resistance NR value ≥ 0.5.

Conclusion. Venous thromboses occur under the condition of expressed APC resistance with underlying FVL(1691)GA carriage. The APC resistance index can serve as an objective biochemical marker to determine the feasibility of thromboprophylaxis within the framework of personalized medicine.
Antihypertensive and vasoprotective effectiveness of the chronopharmacotherapy in patients with arterial hypertension after acute cerebrovascular accident
Authors: Skibitsky V. V. , Fendrikova A. V. , Opolskaya S. V.
Published: February 2019

Aim. To evaluate the eﬀectiveness of combined antihypertensive chronopharmacotherapy and estimate the daily blood pressure profle (BP) parameters, such as: stiﬀness of the vascular wall and central aortic pressure in patients with arterial hypertension (AH) who underwent transient ischemic atack (TIA) or ischemic stroke (IS).

Materials and methods. 235 patients with hypertension who underwent acute cerebrovascular accident were examined. Tere were 116 patients with TIA and 119 with IS. All patients were randomized in 4 groups according to regimen of antihypertensive drugs combination. Te 1st group (n = 59) included patients with AH, who underwent TIA and received indapamide retard 1.5 mg and valsartan 160 mg in the morning regiment of drug therapy. Te 2nd group (n = 57) included patients with AH, who underwent TIA and received indapamide retard 1.5 mg in the morning and valsartan 80 mg twice a day (morning and evening). Te 3rd group (n = 47) included patients with AH, who underwent IS and received indapamide retard 1.5 mg and valsartan at a dose of 160 mg in the morning. Te 4th group (n = 56) included patients with AH, who underwent IS and received indapamide retard 1.5 mg in the morning and valsartan 80 mg twice a day (morning and evening). Ambulatory blood pressure monitoring (ABPM), central aortic pressure (CAP) measurement and vessel wall stiﬀness values were evaluated before treatment and afer 12 months of therapy.

Results.Before the start of combined antihypertensive chronopharmacotherapy, most of the parameters for ABPM, vessel wall stiﬀness values and CAP in groups 1 and 2, 3 and 4 were comparable. Achievement of the target level of BP afer 8 weeks of treatment, was signifcantly more ofen in groups with a double sartan therapy (group 2 and group 4) in compare with its single time application only in the morning hours (group 1 and group 3) (p<0.05). Statistically signifcant positive dynamics of the main values of the daily profle of blood pressure, stiﬀness of the vascular wall and central aortic pressure (p <0,05) were registered in all groups. However, more pronounced decline of main parameters of ABPM, stiﬀness of the vascular wall and central aortic pressure values were noted in group with double use sartan therapy in compare with single time sartan therapy in the morning time. (p <0.05). Signifcant positive dynamics of the main values of the ABPM, stiﬀness of the vascular wall and CAP were registered in patients who underwent IS and received double application sartan therapy (4th group) in compare with patients with TIA (2nd group) (p = 0.02).

Conclusion.Double use sartan therapy, combined with a thiazide diuretics in patients, who underwent IS or TIA more ofen promotes to get target values of blood pressure, improve the main values of the ABPM, stiﬀness of the vascular wall and CAP in compare with single time sartan therapy in the morning time
Antihypertensive therapy in male and female: are there sex differences in choice of drugs?
Authors: Larina V. N. , Orlov D. A.
Published: February 2019

Tis review presents data on prevalence, control, and eﬀectiveness of treatment of hypertension in male and female. Te features of protective action and manifestations of undesirable eﬀects of antihypertensive therapy in male and female, as well as situations inﬂuencing the choice of a drug depending on sex are discussed.
КОМПЬЮТЕРНАЯ МОДЕЛЬ ПРИНЯТИЯ РЕШЕНИЙ ПО РЕКОНФИГУРАЦИИ СТРУКТУРЫ ТЕХНОЛОГИЧЕСКОЙ СИСТЕМЫ ИЗМЕЛЬЧЕНИЯ РУДЫ
Authors: (Seyran Sh. Balasanyan) Баласанян Сейран Шамирович , (Hermine M. Gevorgyan) Геворгян Эрмине Михайловна
Published: February 2019

Актуальность исследования обусловлена необходимостью повышения надежности и эффективности функционирования технологической системы измельчения руды путем введения структурной избыточности. Цель: обоснование необходимости повышения эффективности функционирования технологической системы измельчения руды путем введения структурной избыточности (резервных связей), определение наилучшей структуры резервных связей между измельчительными агрегатами и рациональной стратегии их использования, построение математической модели принятия решений по реконфигурации (перестроении) структуры технологической системы измельчения руды при отказах ее оборудования в виде дерева решений, разработка компьютерной модели принятия решений по реконфигурации структуры технологической системы измельчения руды. Объект: технологическая система измельчения руды Зангезурского медно-молибденового комбината (Армения). Методы: метод дерева решений (метод мягких вычислений), имитационное моделирование. Результаты. В результате имитационных экспериментов с компьютерной модели технологической системы измельчения руды определена наилучшая структура резервных связей между измельчительными агрегатами и рациональная стратегия их использования, с применением которых построена математическая модель принятия решений по реконфигурации структуры технологической системы измельчения руды (при отказах ее оборудования) в виде дерева решений. На основе этой модели разработана компьютерная модель, позволяющая при изменении работоспособ­ности измельчительных агрегатов своевременно выбрать наилучшую для данной сложившей отказовой ситуации структуру и режим работы каждого агрегата, независимо от квалификации и опыта оператора. Благо­даря применению этой модели оператор полностью освобождается от обязанностей лица, принимающего решения. Разработанная компьютерная модель включена в состав специального программного обеспечения автоматизированной системы управления технологическим процессом измельчения руды Зангезурского медно-молибденового комбината (Армения).
ОПТИМИЗАЦИЯ ПРОЦЕССА ЗАКАЧКИ ФИКСИРОВАННЫХ ОБЪЕМОВ ЖИДКОСТИ В ДВА НАПРАВЛЕНИЯ
Authors: (Golia I. Bikbulatova) Бикбулатова Голия Ильдусовна , (Achmetsalim S. Galeev) Галеев Ахметсалим Сабирович , (Yulia A.Boltneva) Болтнева Юлия Анатольевна , (Petr A. Larin) Ларин Пётр Андреевич , Rais N. Suleymanov Сулейманов Раис Насибович , (Oleg V. Filimonov) Филимонов Олег Владимирович
Published: February 2019

Представлен новый подход к решению задачи оптимизации работы динамической системы «насос+трубопроводная сеть». В статье состоянием процесса закачки называются объёмы жидкости, поступившие через каждый трубопровод в данный момент времени. Эти объёмы взяты в качестве основных функций, зависящих от времени и описывающих процесс закачки. Система функций определяет воображаемую линию – траекторию закачки в пространстве, задаваемом плановыми объёмами закачки через каждый трубопровод. Регулирование потока в трубопроводах любыми устройствами, создающими местное гидродинамическое сопротивление (устройствами) даёт возможность получать различные траектории. Влияние регулирующих устройств оценивается коэффициентом открытости трубопровода или потока, определяемым как отношение потока (расхода) в частично перекрытом трубопроводе к потоку в полностью открытом трубопроводе. Рассмотрены два варианта закачки, в которых продемонстрировано, что разным траекториям может соответствовать своя потребляемая энергия. Проведён анализ используемой при количественных исследованиях и оценке работы насоса величины, равной отношению напора, создаваемого насосом, к КПД, соответствующему этому напору. Показано, что при повышении напора или снижении подачи данная величина возрастает. Поскольку затрачиваемая насосом энергия зависит от формы траектории, энергия становится функционалом, для поиска минимума которого привлекаются методы вариационного исчисления. Таким способом находится экстремаль – траектория, на которой затраты энергии и времени минимальны. Траекторией, описывающей оптимальный режим закачки, оказывается прямолинейный отрезок в пространстве закачки, концы которого соответствуют начальному и конечному состояниям процесса закачки. Для реализации данного процесса предложен алгоритм, позволяющий определить, какой трубопровод следует частично перекрыть, и рассчитать меру перекрытия, рассматривая регулирующее устройство как устройство, задающее коэффициент местного сопротивления. Цель работы: определить оптимальный способ закачивания в два трубопровода, в котором насос и трубопроводная сеть рассматриваются как единая взаимосвязанная система. Объекты исследования: насосы, используемые для поддержания пластового давления, два трубопровода, снабжённых устройствами для регулирования расхода жидкости. Методы исследования: метод для нахождения оптимального режима – вариационное исчисление. Результаты. Работа насоса требует наименьшего количества энергии и времени, если заполнение заданных объёмов происходит одновременно. Для процесса экономичного закачивания в два трубопровода дан алгоритм определения степени перекрытия трубопроводов.
УСЛОВИЯ СОВМЕСТНОГО УДАЛЕНИЯ СОЕДИНЕНИЙ КРЕМНИЯ И ГУМИНОВЫХ ВЕЩЕСТВ ИЗ ПРИРОДНЫХ ВОД В ПРИСУТСТВИИ ПРОДУКТОВ ТЕРМИЧЕСКОЙ ОБРАБОТКИ МАГНЕЗИТА
Authors: (Larisa A. Kostikova) Костикова Лариса Анатольевна , (Lyudmila N. Shiyan) Шиян Людмила Николаевна , (Ksenia I. Machekhina) Мачехина Ксения Игоревна , (Andrey V. Korshunov) Коршунов Андрей Владимирович
Published: February 2019

Актуальность. Подземные воды Западно-Сибирского региона характеризуются повышенным содержанием ионов железа, силикат ионов и органических веществ гумусового происхождения. Эти примеси способствуют образованию устойчивой коллоидной системы. При использовании природной воды в качестве теплоносителя на поверхности труб формируются силикатные отложения за счет связывания катионов кальция, магния, алюминия, железа. Совместное присутствие силикатов и органических примесей приводит к снижению эффективности очистки воды с применением существующих технологий водоподготовки. Цель: определение концентрационных и температурных условий образования соединений кремния с гуминовыми веществами и скорости их осаждения в присутствии продуктов термической обработки магнезита (каустического магнезита). Объекты: природные воды и модельные растворы, содержащие соединения кремния и растворенные гуминовые вещества, каустический магнезит. Методы: фотоколориметрия, рН-метрия, титриметрия, атомно-эмиссионная спектрометрия с индуктивно связанной плазмой (ICAP-6000). Результаты. Установлен интервал массовых соотношений растворимых гуминовых веществ и силикатов ГВ:SiO32–=0,8–4, в котором гуминовые вещества и SiO32–-ионы при совместном присутствии в интервале рН=5…9 связываются в органо-минеральные комплексы. В природных высокоцветных водах (содержание органических веществ до 30 мгО/л) до 40 % силикат ионов может быть связано в такие комплексы. Размер образующихся коллоидных частиц составил порядка 200 нм, значение ζ-потенциала составило –38 mV. При использовании каустического магнезита в качестве реагента-осадителя взаимодействие в системе «силикат–гуминовые вещества» протекает в две стадии, определяющие технологическую целесообразность одновременного удаления из воды силикатов и гуминовых веществ.
МЕТОДИЧЕСКОЕ ОБОСНОВАНИЕ ПОВЫШЕНИЯ ВЫРАБОТКИ ЗАПАСОВ НЕФТИ МЕСТОРОЖДЕНИЙ, ОСЛОЖНЕННЫХ ТЕКТОНИЧЕСКИМИ НАРУШЕНИЯМИ
Authors: (Andrey V. Stenkin) Стенькин Андрей Вениаминович , (Yury A. Kotenev) Котенев Юрий Алексеевич , (Shamil K. Sulnatov) Султанов Шамиль Ханифович , (Vil G. Umetbaev) Уметбаев Виль Гайсович
Published: February 2019

Актуальность. Нефтегазовые залежи месторождений Шаимского района осложнены разнопорядковыми тектоническими нарушениями. В статье рассмотрено влияние тектонических нарушений на разработку залежей и выработку запасов нефти из них. Объектом исследования являются продуктивные пласты юрских отложений месторождения Шаимского нефтегазоносного района, характеризующиеся высокой степенью неоднородности и низкими значениями фильтрационно-емкостных свойств, осложнёнными тектоническими нарушениями. Недостижение проектных показателей выработки запасов нефти для подобных месторождений требует проведения детального мониторинга разработки с учетом ключевых геологических характеристик и последующего регулирования и совершенствования системы разработки. Методический подход к совершенствованию системы разработки месторождений, осложненных тектоническими нарушениями, должен включать критический анализ первичной геолого-геофизической и промысловой информации, анализ основных показателей разработки, характеризующих выработку запасов нефти. Цель работы: определение причин неравномерности выработки запасов по площади и разрезу продуктивных пластов, изучение влияния тектонических разломов различного порядка на эффективность реализуемой системы разработки, в том числе и на систему заводнения продуктивных пластов. Методы исследования: геолого-гидродинамическое моделирование разработки, геолого-промысловый анализ разработки, анализ результатов интерпретации гидродинамических исследований скважин, сопоставительный анализ фильтрационно-емкостных свойств пласта, полученных различными методами исследований. Анализ эффективности системы разработки проводился с использованием косвенных методов математической статистики, подкрепленных прямыми методами – трассерными исследованиями скважин. Результаты. На примере Новомостовского нефтяного месторождения выделены гидродинамически обособленные участки с высокими значениями подвижных остаточных запасов, ограниченных тектоническими нарушениями, для которых предложены геолого-технические мероприятия и технологии, направленные на повышение выработки запасов нефти. По результатам проведенных исследований предложено методическое обоснование оперативного регулирования и мониторинга разработки пластов, осложненных тектоническими нарушениями.
Pengaruh Induksi Bakteri Pseudomonas aeruginosa Terhadap Human Umbilical Vein Endothelial Cells (HUVECS) Culture [ The Influence Of Pseudomonas aeruginosa Induction To The Human Umbilical Vein Endothelial Cells (HUVECS) Culture ]
Authors: Nur Hidayati Dwi Yuni
Published: February 2019

Abstract One of the cause of negative gram bacteria is Pseudomonas aeruginosa (Ps. aeruginosa). It continuing become sepsis on the first step of patogenesa happen sticking between aeruginosa with endhotel cell. This sticking is mediated by adhesi molecul which have characterize same with hemaglutin protein. The main study of this research is to know the profile of endothelial cell culture within induction of Ps. aeruginosa. The methods are Ps. aeruginosa (9064) which have been isolated then processes on TCBS Media and continued with isolation terracely. The profile of weight molecule protein obtained by SDS PAGE and then conducted electroelution. The test of hemaglutinin using eritrosit mencit. The receptor cell used is endhotel cell (HUVECs) culture. The result show that dosage of hemaglutin protein of Aeruginosa bring effect to profile of HUVECs culture determined by index adhesion of Ps. aeruginosa to HUVECs culture. We conclude that protein 38,19 kDa give effect to profile of HUVECs culture in vitro.
Uji Toksisiti Asam Α-Linolenik Rumput Laut Ulva sp. Terhadap Chattonella marina Dan Heterosigma akashiwo [Toxicity Assay Of Α-Linolenic Acid From Ulva sp. Seaweed On Chattonella marina And Heterosigma akashiwo]
Authors: Alamsjah Moch. Amin
Published: February 2019

Abstract The higher toxicity of α-linolenic acid from Ulva sp. seaweed showed the high algicidal activity against Chattonella marina and Heterosigma akashiwo. Among six species tested, C. marina and H. akashiwo was the most susceptible to this fatty acid, whereas LC50 of α-linolenic acid was estimated to be 3.22 and 0.58 µg/mL, respectively. Furthermore, α-linolenic acid also showed algicidal activity against Alexandrium tamarense and A. taylori. It is suggested that α-linolenic acid is useful mitigation agents to Harmful Algal Bloom effect, especially on Chattonella marina and Heterosigma akashiwo.
Isolasi dan Identifikasi Bakteri Gram Negatif Pada Luka Ikan Maskoki (Carassius auratus) Akibat Infestasi Ektoparasit Argulus sp. [Isolation and Identification Gram Negative Bacteria At Lessions Of Gold Fish (Carassius auratus) By Infestation Ectoparasite Argulus sp.
Authors: Kismiyati Kismiyati , Subekti Sri , Yusuf R. Wahid Nur , Kusdarwati Rahayu
Published: February 2019

AbstractOne of them is the problem of disease Argulus sp. is ektoparasite which often attacked in activity of good conducting of sea water and also freshwater. Argulus sp. attacking at part of fin, husk, gill and entire all its body exterior of him. attacked by fish is Argulus sp. will seen is thin, often rub body and arise red pock which later can generate secondary infection. Utilize to prove the existence of bacterium which emerge effect of lession (secondary infection) which because of bite of Argulus sp. that is by identifying bacterium and biochemical test. Media which used in identifying bacterium for example that is TSA (Tryptic Soy Agar), TSIA (Triple Soy Iron Agar), MIO (Motility Indol Ornithin), O/F medium (Oksidative/Fermentative), sugar test medium (maltosa, laktosa, arabinosa, inositol, manitol, sukrosa). Target of this research is to know the existence of gram negative bacterium and gram negative bacterium any kind of which is found at fish hurt of gold fish (Carassius auratus) effect of infestation by ectoparasite Argulus sp. Research which is use descriptive method. That is with infestation of Argulus sp. into containing aquarium of gold fish counted 1 tail and Argulus sp. counted 5, 10, 15, 20 tail. After Argulus sp. patch all at gold fish is later then perceived until gold fish arise lession effect of bite of Argulus sp. is afterwards identifyed by bacterium to know the bacterium that were formed on gold fish lesions. The result showed that the lesions of gold fish, isolation at TSA medium and identified by biochemical test to diagnose bacteriums found on gold fish lession. Identifications of the three gram negative bacterium as follow : Aeromonas hydrophilla, Pseudomonas flourescens, Flexibacter columnaris while for the Edwardsiella tarda was not be found in this study
Kelangsungan Hidup Ikan Koi (Cyprinus carpio koi) yang Terinfeksi KHV (Koi herpesvirus) [The Survival Of Koi Goldfish (Cyprinus carpio koi) which Infected By KHV (Koi herpesvirus) ]
Authors: Setyorini Ninik , Khusnah Asmaul , Widajatiningrum Lilia
Published: February 2019

AbstractThe research aim to know the capability of survival life of the infected KHV koi fish that proved with PCR test. The research held from 2003 until 2008, with the methode took gill sample from the same fish every 4 months. Result of PCR showed positive KHV in each analysis during 4.5 years. According to the PCR test for KHV that conducted for 4.5 years showed that the result always positive in every test.
Pengaruh Kombinasi Pupuk Kompos dan NPK Terhadap Pertumbuhan, Jumlah Klorofil A dan Kadar Air Gracilaria verrucosa [Effect Of Compost and NPK Fertilizer Combinations On Growth, Chlorophyll A and Water Content Of Gracilaria verrucose]
Authors: Alamsjah Mochammad Amin , Silviana Iva Nur , Rachmawati Kadek
Published: February 2019

AbstractIn general, Gracilaria culture still using organic and inorganic fertilizer single. Organic or inorganic fertilizer applied is felt have not yet enough to support the growth of Gracilaria. The aim of this experiment was to know the best compost and NPK combinations which could influence on growth, chlorophyll a and water content of G.verrucosa. Treatment applied was compost and NPK combinations with different doses, that is A treatment (0 gr/l :  2 gr/l), B treatment (0,5 gr/l : 1,5 gr/l), C treatment (1 gr/l : 1gr/l), D treatment (1,5 gr/l : 0,5 gr/l), E treatment (2 gr/l : 0 gr/l) and F treatment (without fertilizer), each treatment was replicated 4 times. Main parameter observed was daily growth rate (%/day), relative length growth (%), chlorophyll a (ìg/ml) and water content (%) of G.verrucosa. oSupported parameter observed was pH, temperature ( C) and salinity (‰). Data analysis using Analysis of Variance (ANOVA) and Duncan Multiple Range Test. Result of  the experiment indicates that compost and NPK combinations with different doses was significantly influence to growth, chlorophyll a and water content of G. verrucosa. The highest mean of daily weight growth rates was C treatment (1 gr/l : 1gr/l) which was equal to 2,56%/day and the lowest mean was  F treatment (without fertilizer) which was equal to 1,12%/day. The highest mean of relative length growth was C treatment (1 gr/l : 1gr/l) which was equal to 2,21% and the lowest mean was F treatment (without fertilizer) which was equal to 0,60%. The highest mean of chlorophyll a by the end of the experiment was C treatment (1 gr/l : 1gr/l) which was equal to 0,001948 ìg/ml and the lowest mean was F treatment (without fertilizer) which was equal to 0,001440 ìg/ml. The lowest mean of water content by the end of the experiment was C treatment (1 gr/l : 1gr/l) which was equal to 28,48 % and the highest mean was F treatment (without fertilizer) which was equal to 30,39%.
Pengaruh Pemberian Bahan Aktif Ekstrak Nannochloropsis oculata Terhadap Kadar Radikal Bebas Pada Ikan Kerapu Tikus (Cromileptes altivelis) yang Terinfeksi Bakteri Vibrio alginolyticus [The Influence Of Supply Of Bioactive Extract Nannochloropsis oculata To Dose Of Free Radicals In The Humpback Grouper (Cromileptes altivelis) That Was Infected By Vibrio alginolyticus]
Authors: Yanuhar Uun
Published: February 2019

AbstractNanochloropsis oculata is one of kind green algae that overload in Indonesia ocean. The purpose of this research  was to know the potency antioxidant of Nannochloropsis oculata extract as in the Humback Grouper. The research was conducted by  experimently and used direct random sampling as design with 4 experiments and 3 repeats  i.e control fish, fish was  added Nannochloropsis oculata extract, Vibrio alginolyticus - infected fish, and Vibrio alginolyticus - infected fish that was added Nannochloropsis oculata extract. The result showed, fish that added by Nannchloropsis oculata extract made a different influence which was very clear to the decreasing of  free radical in the body. Free radical (malonaldehid) in the Vibrio alginolyticus -  infected fish was 4, 04 µg/100 mg which fish was added by Nannochloropsis oculata extract, the free radical level was decrease into 3, 29 µg/100 mg massa.
Kandungan Protein Kasar dan Serat Kasar Pada Daun Kangkung Air (Ipomoea aquatica) yang Difermentasi
[Crude Protein Content and Crude Fiber On Watercress Leaf Water (Ipomoea aquatica) in Fermented Processing]
Authors: Agustono Agustono, , Widodo Andy Setyo , Paramita Widya
Published: February 2019

Abstract Watercress leaf water availability (Ipomoea aquatica) in the aquatic environment, can be use as feed supplement, because it has enough protein, but the content is very high crude fiber. Fermentation is a good way to improve the value of water content of watercress leaf water. The purpose of this research to increase the protein content and lower crude fiber content levels. The study was conducted in the Animal Husbandary Departement Faculty of Veterinery Medicine of Airlangga University. This research used watercress leaf water and probiotics as fermenters. Treatments is used 5 with the size of different doses of probiotics, namely P0 (0%), P1 (2%), P2 (4%), P3 (6%), and P4 (8%), of each treatment is repeated 4 times, and the fermentation process carried out for 7 days, then analysed proximat. Experimental design used is Complete Random Design (RAL). Analysis of data using various print and to determine the best treatment performed Duncan's Multiple Range test. The result showed that the use of probiotics in the fermentation process watercress leaf water the real impact of increased crude protein and crude fiber decreased. Crude protein content increased from 23,9945% (P0) to 28,1079 (P4). Crude fiber decreased from 16.1744% (P0) to 11.8341% (P3). The use of probiotics are most effective in the fermentation process watercress leaf water at doses of 8%.
Efektivitas Penggunaan Whole Cell Dari Vibrio alginolyticus Sebagai Vaksin Oral Melalui Artemia Pada Benih Ikan Kerapu Tikus (Chromileptes altivelis)
[Effectiveness Of Using Vibrio alginolyticus Whole Cell As Oralvaccine With Artemia To Humpback Grouper Fry (Chromileptes altivelis) ]
Authors: Suprapto Hari , Sukmawati Titik Desi
Published: February 2019

Abstract Humpback grouper is the one of marine fish which can culture. The major problem of humpback grouper culture is high mortality at short time caused by pathogen infection include virus, bacterial, parasite and fungi. Pathogen infection at humpback grouper fry caused by bacterial from genus Vibrio spp. There was exceeding with vaccination. This research use oral vaccination with whole cell. Vibrio alginolyticus Whole cell as oral vaccine is suggested to prevent high mortality at humpback grouper fry. The purpose of this research is to know the successful about using Vibrio alginolyticus Whole Cell as Oral Vaccine to prevent high mortality of humpback grouper fry (Chromileptes altivelis). The experiment was done June until December 2009 at Gastroenteritis of Laboratory Tropical Disease Centre (TDC) of Airlangga University Surabaya and PT Benur Puteri Situbondo. The methods of this research is descriptive. Major parameter observed in this research was survival rate (%) and Relative Percentage Survival (RPS) of humpback grouper fry. Other parameter observed in this research was water quality include water temperature, pH, water salinity and Dissolved Oxygen (DO). Result of research shows that there are difference of survival rate between fingerling fish vaccination and fingerling fish non vaccination (control). Survival rate of humpback grouper fry which vaccine of Whole Cell 66 % even though survival rate of humpback grouper fry which non vaccine of Whole Cell (control) 46 %. Relative Percentage Survival (RPS) of humpback grouper fry which vaccine of Whole Cell 37 %. The value of RPS was resulted at this research shows low value so that must to perfecting and must to do again research. Water temperature 27-29oC, pH 7,3-7,5, water salinity 31-32 ppt and dissolved oxygen 5-6 ppm.
Isolasi Bakteri Indigen Sebagai Pendegradasi Bahan Organik Pada Media Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup
[Isolation Indigen Bacteria In Organic Materials As Media Degrading Seedling Fish Catfish (Clarias sp.) Closed Recirculation System]
Authors: Arief Muhammad , Sulmartiwi Laksmi , Prayogo Prayogo , Saputri Herlina Maya
Published: February 2019

Abstract Rearing of African catfish fry requires an abundant water supply, so this causes Clarias gariepinus hatchery operations difficult in areas that lack water, so use a closed recirculation system technology which is an attempt to tackle the problem of limited water in an area for hatchery operations. However, these systems have drawbacks which deposition of organic materials on water quality pond maintenance reduced the need for water quality management. Components of organic materials is a source of water quality pollutants consisting of proteins, lekam, and carbohydrates, so that in the organic material needed pendegradasian existence of groups of bacteria and proteolytic bacteria, lipolytic, and amylolitic. The results of this study show that in the media of African catfish hatchery fish have bacteria that degrade organic material that has properties of proteolytic, lipolytic, and most potentially amylolitic consisting of bacterial isolates of Pseudomonas stutzeri B ie (97.81%) with values highest protease hydrolysis test results 1.878 mm, ie G isolates of Pseudomonas pseudomallei (97.81%) with the highest value of 1.909 mm hydrolysis test and bacterial isolates of Pseudomonas stutzeri K (61.21%) with the highest value of 1.944 mm hydrolysis test.
Sargassum Sp. Sebagai Biokontrol Terhadap Kandungan Logam Berat Timbal (Pb) Yang Terserap Oleh Kerang Darah (Anadara granosa)
[Sargassum Sp. As Biocontroll To Timbale (Pb) Heavy Metal Contens That Absorbed By Shell (Anadara granosa) ]
Authors: Alamsjah Mochammad Amin , Rakhmat Bagus , Cahyoko Yudi , Sudarno Sudarno
Published: February 2019

Abstract Shell is aquatic organism class of Molluscs as non selective filter feeder and also as seafood main menu. In other side many food poisoning case was found as result consuming of Shell. Timbale (Pb) is one of very dangerous heavy metal and able to caused of poisoning in organism. According to PP RI Number 82/2001 Pb concentration that was suitable with maximum limit of aquatic quality is 0,03 ppm. Sargassum sp. also was knowned have been absorbing ability for heavy metal like Pb that spread in aquatic. Hopeable with a Sargassum sp. as biocontroll model on timbale heavy metal contens is able to prevent food poisoning causing contaminated shell consumtion. This research was done in Fisheries Laboratorium Fisheries and Marine Faculty Airlangga University on 15th – 11th 2010. The research method is experiment with design of experiment that used is Completely Randomized Design Factorial with three replicates (I, II and III) and consist of two factors, that is Pb(NO3)2 in concentration 0.015 ppm, 0.03 ppm and 0.06 ppm, Sargassum sp. in weight 100 g, 200 g and 400 g. Data analysis using Analysis of Variance (ANOVA), if there are any different it could be continued with Honestly Significant Difference (HSD) Test to known interaction between treatments. Result of the research indicates that Pb dose is significantly (p<0.05) to Pb contens number that absorp by Sargassum sp. among research. It’s supported by HSD test indicates that highest result is A3 (Pb(NO3)2 0,06 ppm) that significantly with A2 (Pb(NO3)2 0,03 ppm) and A1 (Pb(NO3)2 0,015 ppm). Sargassum sp. is also be able to used as biocontroll to timbale heavy metal contens that absorbed by shell, because in low and highest dose Sargassum sp. absorption capacity to the timbale always bigger than shell absorption capacity . In other side, there are no interaction between timbale dose and weight of Sargassum sp. to timbale contens that absorbed by shell.
Penambahan Suplemen Madu dalam Pakan Guna Meningkatkan Pertumbuhan Dan Kelulushidupan Benih Lobster Air Tawar Red Claw (Cherax quadricarinatus)
[The Addition Supplement Of Honey In Feed To Increasing Growth And Survival Rate Of Freshwater Crayfish Seed Red Claw (Cherax quadricarinatus) ]
Authors: Mukti Akhmad Taufiq , Aprilia Fachriza Tri , Rahmahani Jola , Arief Muhammad
Published: February 2019

Abstract The demand for domestic or worldwide market to freshwater crayfish is high. Wherefor, needed some cultivation to reach that demand. The growth period of freshwater crayfish is slowly, then to increase growth needed addition some supplement in the feed. The aim of this research was to find out the influence of addition honey in the feed concerning to growth and survival rate freshwater crayfish seed red claw (C. quadricarinatus) and also to figure out the best dose of addition honey which one could increase growth and survival rate of freshwater crayfish red claw (C. quadricarinatus) seed. The method in the research was used Completely Randomized Design with 9 treatment. The addition doze of honey in every feed is different such as A (0 ml/kg of feed) as control, B (300 ml/kg of feed), C (400 ml/kg of woof), D (500 ml/kg of feed), E (600 ml/kg of feed), F (700 ml/kg of feed), G (800 ml/kg of feed), H (900 ml/kg of feed) dan I (1000 ml/kg of feed), in every treatment must repeated 3 times. The result of this research figure out that an addition honey in feed of freshwater crayfish red claw (C. quadricarinatus) was not obviously influence to growth and also survival rate. The best doze of honey to add in feed of freshwater crayfish red claw (C. quadricarinatus) to reach maximal weight growing up is 800 ml/kg of feed.
Pengaruh Penggunaan Kombucha Terhadap Kandungan Protein Kasar Dan Serat Kasar Pada Fermentasi Eceng Gondok (Eichornia crassipes)
[The Effect Of Using Kombucha On Crude Protein And Crude Fiber Contents In Water Hyacinth (Eichornia crassipes) Fermentation]
Authors: Agustono Agustono , Hidayat Salim , L Widya Paramita
Published: February 2019

Abstract Water hyacinth (Eichornia crassipes) is a one of the feed materials from the green plants a potential. Howefer, the water hyacinth are low of protein and high fiber contents. Therefore, there should be efforts intentionally done to increase the leaf protein. One the ways to raise water hyacinth nutrient is performing biologically fermentation by using microbes. The Kombucha organism is a symbiotic colony of yeast’s and bacteria that composed by strong membrane that covers gelatinous mass. Kombucha microorganism is living together with permeate the tea drink. The objective of this research is to know the effect of Kombucha to increased crude protein and decreased crude fiber contents in water hyacinth fermentation. The advantage of the research is to provide information regarding a Kombucha dosage which is required to enhance crude protein and reduce crude fiber content on water hyacinth fermentation. The research used water hyacinth as substrate and Kombucha as fermentor. Kombucha dosaged used here were P0 (0%), P1 (7,5%), P2 (15%) and P3 (22,5%). The fermentation took place 7 days and then proceeded with proximate analysis. Variables observed consisted of crude protein and crude fiber contents after being fermented using Kombucha. The result showed that Kombucha that was used for fermenting water hyacinth, could increase the crude protein content from 13,3040 % (P0) to 15,9972% (P3) and not significantly reduce the crude fiber. The Kombucha dosage at 7,5% (P1) was most effective for fermenting the water hyacinth.
Kandungan Gizi dan Karakterisasi Senyawa Bioaktif Lintah Laut (Discodoris sp.)
[Nutritional Content and Characterization Bioactive Compounds of Sea Sluge (Discodoris sp.) ]
Authors: Hafiluddin Hafiluddin , Nurjanah Nurjanah , Nurhayati Tati
Published: February 2019

Abstract One of the commodities produced from the ocean that have a bioactive compounds is a sea slug (Discodoris sp.). That is very interesting to study mainly deals with the nature of both chemical and biochemical characteristics and their use for the field of food and health. The aims of this research was to determine the nutrient content of sea slug, determaine chemical content and determine antioxidant activity. The experiment was conducted with several stages: sample preparation, analysis of nutrient content, extraction bioactive compound, analysis of chemical content and antioxidant activity.  Sea slug from the island of Madura Pamekasan potential as a source of protein, fat, and minerals. Sea slug has esensial amino acids amount to 5.57% was dominated by leucin of 1.42%. Nonesensial amino acids amount to 6.54% wich was dominated by glutamic acid 2.19%.  Saturated fatty acids amounted to 27.53% of sea slug was dominated by palmitic (C16: 0) which was 13.36%. Unsaturated fatty acids amounted to 34.66%, which was dominated by the essential fatty acids linolenic (C18: 3, n-3) 20.91%. The highest yield of the crude extract was ethanol and contained alkaloid compounds, steroids, phenols, carbohydrates and reducing sugar compound. The meat of sea slug with ethanol solvent has an IC best 50 antioxidant activity at 441, 12 ppm.
Pengaruh Pemberian Bakteri Bacillus pumilus pada kotoran Sapi Sebagai Pupuk terhadap Jumlah Kandungan Klorofil Dunaliella salina
[The Effect of Bacteria Bacillus pumilus In Cow Dung As Fertilizer to Total Chlorophyll Dunaliella salina]
Authors: Masithah Endang Dewi , Ningrum Nuansa Adharia , Sigit Setiawati
Published: February 2019

AbstractDunaliella salina is one type of natural food used in the hatchery business. Production stability D. salina with an abundance of nutrients can be supported. The purpose of this study was to determine the effect and how many doses of the bacteria Bacillus pumilus best fermented in cow dung as fertilizer in increasing the population of D. salina. Research conducted at the Laboratory of Education Faculty of Fisheries and Marine Fisheries, Airlangga University Surabaya. The research design used was Completely Randomized Design (CRD) is followed by Duncan test. D. salina was cultured in 250 mL glass bottle with 5 treatments (2 treatments as a control) and four replications. Culture medium used contained 10 ppm cow dung. The concentration of cow manure are given in the study, namely A (12.5% Bacillus pumilus), B (10% Bacillus pumilus), C (7.5% Bacillus pumilus), control 1 (without fermentation), control 2 (fertilizer Walne). The results showed that the addition of Bacillus pumilus on cow dung that were cultured in culture medium D. salina can increase the amount of chlorophyll content of D. salina. Addition of Bacillus pumilus by 10% in the culture medium to produce the amount of chlorophyll-a D. salina high of 0.014055 µg/mL and chlorophyll-b of 0.009657142 µg/mL on the first day.
Pengaruh Konsentrasi Air Kelapa Muda dan Madu dalam NaCl Fisiologis terhadap Motilitas dan Lama Hidup Spermatozoa Ikan Patin (Pangasius pangasius)
[The Effect of Concentration Young Coconut Water and Honey in 0,9% Sodium Chloride to Motility and Life Time Catfish (Pangasius pangasius) Spermatozoa]
Authors: Sulmartiwi Laksmi , Ainurrohmah Eka , Mubarak A. Shofy
Published: February 2019

Abstract Ability nature maturation of catfish just one time a year at early rainy season (November-March) and nature seed population was down because catching, so to solve the probleme is storage of catfish spermatozoa and can used as need. Young coconut water and honey as thinner in storage catfish spermatozoa content glucose and fructose result ATP as energy resources. Destination of this study to know effect of young coconut water and honey to catfish spermatozoa motility and live time in storage process. Study method is experiment with Completely Randomization Design as experimental design. With different treatment young coconut water is A (0%), B (19,4%), C (39,4%), D (59,4%), E (79,4%) and F (99,4%), and add 0,6% honey at every treatment. Save the treatment at 5-7°C for 36 hours with checked every 4 hours. Data analyzed with Anova and continued by Duncan’s Multiple Range Test. Result this study shown used young coconut water with different concentration is not different significant at motility but different significant at live time catfish spermatozoa. Young coconut water with concentration 99,4% and honey 0,6% influence at catfish spermatozoa live time.
Pengaruh Ekstrak Alga Cokelat (Sargassum sp.) terhadap Pertumbuhan Bakteri Escherichia coli Secara In Vitro
[Effect of Brown Algae Extract (Sargassum sp.) on Growth of Escherichia coli In Vitro]
Authors: Alamsjah Moch. Amin , Nurhayati Dwi , Tjahjaningsih Wahju
Published: February 2019

Abstract Algae Sargassum sp. showed the ability to inhibit the growth of a maximum of several types of pathogenic bacteria such as Escherichia coli. Because of Sargassum sp. have antibacterial with 2 active ingredients are compounds of phenol and tannin fiber. So Sargassum sp. potential to serve as antibakteri substances against several types of bacterial pathogens such as bacteria that cause diarhea.The aim of the study was to determine antibacterial activity brown algae exstract (Sargassum sp.) and determine best concentration from brown algae exstract (Sargassum sp.) to kill of E. coli. This study was conducted at several places such as Saints and Technology Faculty and Veterinary Faculty, Airlangga University Surabaya. Research was done September 2010. This study was conducted in the laboratory using experimental method completely randomized design with twelve treatments and there replications. The main objectives of this study were MBC extract of Sargassum sp. Result of the study analyzed with Khi-Khuadrat. Result of the study showed that extract Sargassum sp. has antimicrobial activity against E. coli were determined with in vitro method. Based on MBC data were found to be effective to kill of E. coli at 50 %. Exploration potential of extracts from brown algae (Sargassum sp.) against various species of bacteria that cause diarrhea are expected to become a new information about the content of extracts of Sargassum sp. as an antibacterial. Also from this research will be obtained an alternative treatment of diarrheal diseases that come from living marine resources and will eventually increase the economic value of fishery commodities which have not explored its use as Sargassum sp.
Pengaruh Penggunaan Tepung Daging Bekicot (Achatina fulica) pada Pakan Buatan terhadap Pertumbuhan, Rasio, Konversi Pakan dan Tingkat Kelulushidupan Benih Ikan Patin (Pangasius pangasius)
[Effect of Use of Snail Meat (Achatina fulica) for Artificial Diet on Growth, Food Convertion and Survival Rate Catfish (Pangasius pangasius)]
Authors: Rahardja Boedi Setya , Sari Devieta , Alamsjah Moch. Amin
Published: February 2019

AbstractCurrently catfish is experiencing a fairly rapid growth, this is because the catfish is one of the important commodities in the fishery. Efforts to increase catfish production is to improve the quality of feed. The aim of this study is to observe effect of snail meat meal for growth rate, food conversion ratio and survival rate of catfish seeds. This research method is experimental with a Completely Randomized Design (CRD) with five treatments and four replications. The treatments were used: (A) 0% snail meat meal, (B) 10% snail meat meal and 30% fish meal, (C) 20% snail meat meal and 20% fish meal, (D) 30% snail meat meal and 10% fish meal and (E) 40% snail meat meal. The main parameters measured were growth, feed conversion ratio and survival rate. Supporting parameters measured were water quality. Analysis of the data used is Analysis of variance (ANOVA) and to know the difference among treatment using Duncan's Multiple Range Test. The results of this study showed that use of snail meat meal in feed formulation stastistically difference (p<0.05) for growth and survival rate of catfish seeds but not statistically difference (p>0.05) for feed conversion ratio. Water quality in maintenance media seed catfish is the temperature 0range between 26-31C, pH 7-8 and dissolved oxygen was 7.2-8.0 mg/L.
Tingkat Kerentanan Lingkungan Pesisir Selatan Kabupaten Bangkalan Terhadap Potensi Tumpahan Minyak (Oil Spill)
[Level of South Coastal Area of Bangkalan Residence on Oil Spill Potention]
Authors: Sulistiono, Vincentius P Siregar Maulinna K Wardhani,
Published: February 2019

AbstractThe South Coastal Area of Bangkalan Residence is a dynamic area with potention of oil spill pollution. These are showed that activation of port construction, the activity of mining and other infrastructures. The aim of the research was to determine sensitivity level of South Coastal Area of Bangkalan Residence on oil spill potention as one of mitigation effort. In generally, the result of environment sensitivity index showed that South Coastal Area of Bangkalan Residence is a sensitivity area on oil spill.