bluuebunny/crossref_metadata_2025 huggingface dataset:
Urban Construction Waste Recycling Path: Robust Optimization
Authors: Wu Fan , Mei Shue , Xu Haiying , Hsu Wei-Ling
Published: November 2023

The world produces a huge amount of urban construction waste each year. Scientific planning of the construction waste recycling path is urgently needed to improve the recycling of construction waste. Existing construction waste recycling models do not pay sufficient attention to the uncertainty of the recycling quantity, which limits their ability to provide support for solving practical problems. The purpose of this paper is to solve the problem of uncertain recycling quantities in optimizing the urban construction waste recycling path. Thus, this paper first builds a recycling model for a deterministic environment with the economic objective as the decision criterion and the transportation flow, construction waste treatment capacity and capability, and environmental and social impact as the constraints. Then, a robust optimization method is adopted to optimize the deterministic model for the uncertainty of the recycling quantity. The data of this paper are from Nanjing, China. The validity of the model and the evolution of the recycling path are tested based on the data of Nanjing. The findings of this paper are as follows: Firstly, the robust model is cost-effective in the face of uncertainty in supply. Secondly, the robust model has greater total treatment capacity. Even in the worst-case scenario, it can guarantee a higher treatment capacity. Thirdly, both models follow the proximity principle which reduces the transportation costs and only slowly increases the total cost of the robust model. This paper provides a scientific and convenient tool to plan the recycling path of construction waste in large cities.
Assessment of Safety of Masonry Buildings near Deep Excavations: Ultimate Limit States
Authors: Jasiński Radosław , Harabinova Slavka , Kotrasova Kamila , Skrzypczak Izabela
Published: November 2023

It is a common practice to construct new buildings in the close vicinity of existing buildings. New buildings require deep excavations, which cause non-uniform displacement of the ground, causing a negative impact on the safety and technical conditions of adjacent buildings, including masonry buildings. The fundamental condition to verify structural safety is the knowledge of impacts and the load-bearing capacity of non-strengthened or strengthened structures. Safety is provided via strengthening the structure against deformations related to the ground displacement or by reducing non-uniform displacements of the building structure. This paper focuses on strengthening the ground and underground parts of masonry buildings. It also describes general requirements for providing safety of buildings according to the standard Eurokode 0 and the simplified method for protecting building structures with the use of steel tie rods. Based on the design methods for masonry structures specified in Eurocode 6 and the know-how of the authors in the field of protecting buildings in mining areas, the original method was proposed to determine the required area of reinforcement in the form of steel rods. Also, the original methods were introduced to verify ULS for inclined walls primarily under vertical load and shearing of pillars between openings. In addition to these analytical methods, this paper also illustrates methods for strengthening the ground and underground parts of masonry buildings. Presented in this paper, original solutions used to determine the strengthening of masonry structures and original models used to verify ULS for deflected walls primarily under vertical load can be directly employed in the design practice. The standard criteria were applied and the effects of building deflections, which are not specified in Eurocode 6, were considered.
A Study of the Effects of Handedness on Portable Angle Grinder Risk
Authors: Choi Sung Bum , Park Jong Yil
Published: November 2023

This study investigates the underlying cause of occupational hazards for left-handed construction workers when using portable angle grinders on construction sites. The study was conducted through a survey of 42 participants in South Korean construction companies to gather information on their tasks involving portable angle grinders. The survey covered handle preferences, grip strength assessment, and work posture observations. Furthermore, a qualitative comparison of the work risk for left-handed and right-handed construction workers using a handheld angle grinder for cutting was conducted. Results showed that the grip strength of a left-handed worker’s dominant and non-dominant hands did not significantly vary, implying that grip strength does not significantly affect their work performance. However, left-handed workers exhibited a higher likelihood of accidents due to poor work postures. Improvements to workplace safety by ensuring the use of ambidextrous tools and promoting tailored safety measures and training for left-handed workers were recommended.
Endoprosthetic Reconstruction of the Proximal Humerus with an Inverse Tumor Prosthesis
Authors: Rachbauer Anna Maria , Schneider Kristian Nikolaus , Gosheger Georg , Deventer Niklas
Published: November 2023

Reconstructing the proximal humerus after tumor removal is challenging due to muscle and bone loss. The current methods often result in poor shoulder function. This study assessed the long-term functional and oncological outcomes of using an inverse proximal humerus prosthesis in 46 patients with bone tumors. The results showed a mean range of motion of 62° in anteversion, 28° in retroversion, and 55° in abduction. Notably, 23 patients achieved over 90° of shoulder abduction, with an average of 140°. The median Musculoskeletal Tumor Society Score was 25. Complications included infection in two radiotherapy patients and single dislocations in seven patients. One patient with recurrent dislocations needed revision surgery. In conclusion, the use of the inverse proximal humerus prosthesis in bone tumor treatment yields excellent shoulder function and high patient satisfaction. This approach is especially beneficial for those with metastatic disease.
“Is My Brain Ever Going to Work Fully Again?”: Challenges and Needs of Cancer Survivors with Persistent Cancer-Related Cognitive Impairment
Authors: Haywood Darren , Dauer Evan , Baughman Frank , Lawrence Blake , Rossell Susan , Hart Nicolas , O’Connor Moira
Published: November 2023

Many cancer survivors experience cognitive impairments that impact memory, concentration, speed of information processing, and decision making. These impairments, collectively known as cancer-related cognitive impairments (CRCIs), are a key domain of unmet needs and can significantly impact a cancer survivor’s identity and quality of life. However, there are no purpose-built, multi-domain, needs assessment tools specifically for CRCI. The development of such tools requires an in-depth understanding of cancer survivors’ CRCI-specific challenges and associated needs. This study explored the challenges and associated needs of cancer survivors with persistent CRCI. An in-depth qualitative design using semi-structured interviews with (a) cancer survivors with perceived CRCI (n = 32) and (b) oncology health professionals (n = 19) was utilised. A reflexive thematic analysis of the interviews resulted in five overarching themes: (1) executing regular activities, (2) relational difficulties, (3) occupational functioning, (4) psychological distress, and (5) social functioning, as well as an additional informational needs domain. Ultimately, CRCI was found to directly produce a range of challenges that negatively, and persistently, impact cancer survivors’ quality of life. Cancer survivors were also found to have a range of needs associated with these challenges. This research should be used to inform future challenges and needs assessment tools as well as treatment and supportive care priority areas directly relating to CRCI.
Multiparametric Ultrasound for Diagnosing Testicular Lesions: Everything You Need to Know in Daily Clinical Practice
Authors: Pozza Carlotta , Tenuta Marta , Sesti Franz , Bertolotto Michele , Huang Dean Y. , Sidhu Paul S. , Maggi Mario , Isidori Andrea M. , Lotti Francesco
Published: November 2023

Background: Ultrasonography (US) represents the gold standard imaging method for the assessment of testicular lesions (TL). The gray-scale (GSUS) and color-Doppler (CDUS) ultrasound examination allow sonographers to investigate the size, margins, echotexture, and vascular features of TLs with the aim to differentiate benign from malignant lesions. Recently, the use of contrast-enhanced US (CEUS) and sonoelastography (SE) has led to further improvements in the differential diagnosis of TL. Although GSUS and CDUS are often sufficient to suggest the benign or malignant nature of the TL, CEUS can be decisive in the differential diagnosis of unclear findings, while SE can help to strengthen the diagnosis. The contemporary combination of GSUS, CDUS, CEUS, and SE has led to a new diagnostic paradigm named multiparametric US (mp-US), which is able to provide a more detailed characterization of TLs than single techniques alone. This narrative and pictorial review aimed to describe the mp-US appearance of several TLs. Methods: An extensive Medline search was performed to identify studies in the English language focusing on the mp-US evaluation of TLs. Results: A practical mp-US “identity card” and iconographic characterization of several benign and malignant TLs is provided herein. Conclusions: The mp-US characterization of TL reported herein can be useful in daily clinical practice.
The Geriatric Nutritional Risk Index Predicts Prognosis in Japanese Patients with LATITUDE High-Risk Metastatic Hormone-Sensitive Prostate Cancer: A Multi-Center Study
Authors: Naiki Taku , Takahara Kiyoshi , Watanabe Hiromitsu , Nakane Keita , Sugiyama Yosuke , Koie Takuya , Shiroki Ryoichi , Miyake Hideaki , Yasui Takahiro
Published: November 2023

Malnutrition is associated with prognosis in cancer. The geriatric nutritional risk index (GNRI), based on the ratio of actual to ideal body weight and also serum albumin level, is a simple screening tool for assessing nutrition. We investigated the GNRI as a prognostic factor for oncological outcomes in patients with high-risk metastatic hormone-sensitive prostate cancer (mHSPC) using a Japanese multicenter cohort. This study included a total of 175 patients with LATITUDE high-risk mHSPC, of whom 102 had received androgen deprivation therapy (ADT) plus upfront abiraterone acetate, and 73 had received ADT plus bicalutamide (Bica), from 14 institutions associated with the Tokai Urologic Oncology Research Seminar. Patients were classified into GNRI-low (<98) or GNRI-high (≥98) groups. The GNRI was based on the body mass index and serum albumin level. Kaplan–Meier analysis revealed that the median overall survival (OS) of a GNRI-low group (median 33.7 months; 95% confidence interval [CI]: 26.2–not reached [NR]) was significantly worse than that of a GNRI-high group (median: NR; 95% CI: NR–NR; p < 0.001). Multivariate analysis identified Bica and low GNRI (<98) as independent prognostic factors for reduced times to both castration-resistant prostate cancer and OS, and, therefore, a poor prognosis. Our findings indicate the GNRI may be a practical prognostic indicator in the evaluation of survival outcomes in patients with LATITUDE high-risk mHSPC.
CD46–ADC Reduces the Engraftment of Multiple Myeloma Patient-Derived Xenografts
Authors: VanWyngarden Michael J. , Walker Zachary J. , Su Yang , Perez de Acha Olivia , Stevens Brett M. , Forsberg Peter A. , Mark Tomer M. , Matsui William , Liu Bin , Sherbenou Daniel W.
Published: November 2023

An antibody–drug conjugate (ADC) targeting CD46 conjugated to monomethyl auristatin has a potent anti-myeloma effect in cell lines in vitro and in vivo, and patient samples treated ex vivo. Here, we tested if CD46–ADC may have the potential to target MM-initiating cells (MM-ICs). CD46 expression was measured on primary MM cells with a stem-like phenotype. A patient-derived xenograft (PDX) model was implemented utilizing implanted fetal bone fragments to provide a humanized microenvironment. Engraftment was monitored via serum human light chain ELISA, and at sacrifice via bone marrow and bone fragment flow cytometry. We then tested MM regeneration in PDX by treating mice with CD46–ADC or the nonbinding control–ADC. MM progenitor cells from patients that exhibit high aldehyde dehydrogenase activity also have a high expression of CD46. In PDX, newly diagnosed MM patient samples engrafted significantly more compared to relapsed/refractory samples. In mice transplanted with newly diagnosed samples, CD46–ADC treatment showed significantly decreased engraftment compared to control–ADC treatment. Our data further support the targeting of CD46 in MM. To our knowledge, this is the first study to show preclinical drug efficacy in a PDX model of MM. This is an important area for future study, as patient samples but not cell lines accurately represent intratumoral heterogeneity.
Risk of Second Tumors in Retinoblastoma Survivors after Ionizing Radiation: A Review
Authors: Figueiredo Diana , Marques Inês A. , Pires Ana Salomé , Cavaleiro Claudia F. , Costa Luís C. , Castela Guilherme , Murta Joaquim N. , Botelho Maria Filomena , Abrantes Ana Margarida
Published: November 2023

Retinoblastoma (RB) is the most common ocular neoplasm in children, whose development depends on two mutational events that occur in both alleles of the retinoblastoma susceptibility gene (RB1). Regarding the nature of these mutational events, RB can be classified as hereditary if the first event is a germline mutation and the second one is a somatic mutation in retina cells or nonhereditary if both mutational events occur in somatic cells. Although the rate of survival of RB is significantly elevated, the incidence of second malignant neoplasms (SMNs) is a concern, since SMNs are the main cause of death in these patients. Effectively, RB patients present a higher risk of SMN incidence compared to other oncology patients. Furthermore, evidence confirms that hereditary RB survivors are at a higher risk for SMNs than nonhereditary RB survivors. Over the decades, some studies have been performed to better understand this subject, evaluating the risk of the development of SMNs in RB patients. Furthermore, this risk seems to increase with the use of ionizing radiation in some therapeutic approaches commonly used in the treatment of RB. This review aims to clarify the effect of ionizing radiation in RB patients and to understand the association between the risk of SMN incidence in patients that underwent radiation therapy, especially in hereditary RB individuals.
Transplant Oncology: An Emerging Discipline of Cancer Treatment
Authors: Abdelrahim Maen , Esmail Abdullah , Abudayyeh Ala , Murakami Naoka , Victor David , Kodali Sudha , Cheah Yee Lee , Simon Caroline J. , Noureddin Mazen , Connor Ashton , Saharia Ashish , Moore Linda W. , Heyne Kirk , Kaseb Ahmed O. , Gaber A. Osama , Ghobrial Rafik Mark
Published: November 2023

Transplant oncology is an emerging concept of cancer treatment with a promising prospective outcome. The applications of oncology, transplant medicine, and surgery are the core of transplant oncology to improve patients’ survival and quality of life. The main concept of transplant oncology is to radically cure cancer by removing the diseased organ and replacing it with a healthy one, aiming to improve the survival outcomes and quality of life of cancer patients. Subsequently, it seeks to expand the treatment options and research for hepatobiliary malignancies, which have seen significantly improved survival outcomes after the implementation of liver transplantation (LT). In the case of colorectal cancer (CRC) in the transplant setting, where the liver is the most common site of metastasis of patients who are considered to have unresectable disease, initial studies have shown improved survival for LT treatment compared to palliative therapy interventions. The indications of LT for hepatobiliary malignancies have been slowly expanded over the years beyond Milan criteria in a stepwise manner. However, the outcome improvements and overall patient survival are limited to the specifics of the setting and systematic intervention options. This review aims to illustrate the representative concepts and history of transplant oncology as an emerging discipline for the management of hepatobiliary malignancies, in addition to other emerging concepts, such as the uses of immunotherapy in a peri-transplant setting as well as the use of circulating tumor DNA (ctDNA) for surveillance post-transplantation.
Correlation Analysis on Anatomical Variants of Accessory Foramina in the Sphenoid Bone for Oncological Surgery
Authors: Palamenghi Andrea , Cellina Michaela , Cè Maurizio , Cappella Annalisa , Sforza Chiarella , Gibelli Daniele
Published: November 2023

The sphenoid bone presents several anatomical variations, including accessory foramina, such as the foramen meningo-orbitale, the foramen of Vesalius, the canaliculus innominatus and the palatovaginal canal, which may be involved in tumor invasion or surgery of surrounding structures. Therefore, clinicians and surgeons have to consider these variants when planning surgical interventions of the cranial base. The prevalence of each variant is reported in the published literature, but very little information is available on the possible correlation among different variants. Here, 300 CT scans of patients (equally divided among males and females) were retrospectively assessed to investigate the presence of the foramen meningo-orbitale, the foramen of Vesalius, the canaliculus innominatus and the palatovaginal canal. Possible differences in the prevalence of each accessory foramen according to sex were assessed, as well as possible correlations among different variants through the Chi-square test (p < 0.01). Overall, the prevalence of the foramen meningo-orbitale, the foramen of Vesalius, the canaliculus innominatus and the palatovaginal canal was 30.7%, 67.7%, 14.0% and 35.3%, respectively, without any difference according to sex (p > 0.01). A significant positive correlation was found between the foramen of Vesalius and canaliculus innominatus, both in males and in females (p < 0.01). In detail, subjects with canaliculus innominatus in 85.7–100.0% of cases also showed the foramen of Vesalius, independently from sex and side. The present study provided novel data about the prevalence of four accessory foramina of the sphenoid bone in an Italian population, and a correlation between the foramen of Vesalius and the canaliculus innominatus was found for the first time. As these accessory foramina host neurovascular structures, the results of this study are thus useful for appropriate planning surgical procedures that are tailored to the anatomical configuration of the patient and for improving techniques to avoid accidental injuries in cranial base surgery. Knowledge of the topography, frequencies and the presence/absence of these additional foramina are pivotal for a successful procedure. Clinicians and surgeons may benefit from these novel data for appropriate recognition of the variants, decision-making, pre-operative and treatment planning, improvement of the procedures, screening of patients and prevention of misdiagnosis.
Prognostic Significance of C-Reactive Protein in Lenvatinib-Treated Unresectable Hepatocellular Carcinoma: A Multi-Institutional Study
Authors: Okumura Taiki , Kimura Takefumi , Iwadare Takanobu , Wakabayashi Shun-ichi , Kobayashi Hiroyuki , Yamashita Yuki , Sugiura Ayumi , Joshita Satoru , Fujimori Naoyuki , Kunimoto Hideo , Komatsu Michiharu , Fukushima Hideki , Mori Hiromitsu , Umemura Takeji
Published: November 2023

Background: Serum C-reactive protein (CRP) is an established biomarker for acute inflammation and has been identified as a prognostic indicator for hepatocellular carcinoma (HCC). However, the significance of the serum CRP level, specifically in HCC patients treated with lenvatinib, remains unclear. Methods: We retrospectively analyzed 125 HCC patients who received lenvatinib treatment at six centers. Clinical characteristics were assessed to identify clinical associations between serum CRP and HCC prognosis. Results: The median overall serum CRP level was 0.29 mg/dL. The cohort was divided into two groups: the low-CRP group with a serum CRP < 0.5 mg/dL and the high-CRP group with a serum CRP ≥ 0.5 mg/dL. The low-CRP group exhibited significantly longer overall survival (OS) than the high-CRP group (22.9 vs. 7.8 months, p < 0.001). No significant difference was observed for progression-free survival (PFS) between the high- and low-CRP groups (9.8 vs. 8.4 months, p = 0.411), while time-to-treatment failure (TTF) was significantly longer in the low-CRP group (8.5 vs. 4.4 months, p = 0.007). The discontinuation rate due to poor performance status was significantly higher in the high-CRP group (p < 0.001). Conclusion: A baseline serum CRP level exceeding 0.5 mg/dL was identified as an unfavorable prognostic factor in HCC patients receiving lenvatinib treatment.
Special Issue on Cancer Smart Nanomedicine
Authors: Pinheiro Marina , Farooqi Ammad Ahmad
Published: November 2023

In this Special Issue entitled “Cancer Smart Nanomedicine”, we have gathered high-quality contributions related to the fascinating field of nanomedicine [...]
A Comparison of the Antitumor Efficacy of Novel Multi-Specific Tribodies with Combinations of Approved Immunomodulatory Antibodies
Authors: Manna Lorenzo , Rapuano Lembo Rosa , Yoshioka Asami , Nakamura Koji , Passariello Margherita , De Lorenzo Claudia
Published: November 2023

Many advances in antitumor therapies have been achieved with antagonistic antibodies targeting the programmed cell death protein 1 (PD-1) or its ligand (PD-L1); however, many cancer patients still develop resistance to anti–PD-1/PD-L1 treatments often associated with the upregulation of other immune checkpoints such as Lymphocyte Activation Gene-3 (LAG-3). In order to verify whether it is possible to overcome these limits, we analyzed and compared the effects of combinations of the clinically validated anti-LAG-3 mAb (Relatlimab) with anti-PD-1 (Pembrolizumab) or anti-PD-L1 (Atezolizumab) monoclonal antibodies (mAbs) with those of novel bispecific tribodies (TRs), called TR0304 and TR0506, previously generated in our lab by combining the binding moieties of novel human antibodies targeting the same ICs of the mentioned mAbs. In particular, TR0304, made up of a Fab derived from an anti-PD-L1 mAb and two single-chain variable fragments (scFvs) derived from an anti-LAG-3 mAb, was tested in comparison with Relatlimab plus Atezolizumab, and TR0506, made up of an antigen-binding fragment (Fab) derived from the same anti-LAG-3 mAb and two scFvs derived from an anti-PD-1 mAb, was tested in comparison with Relatlimab and Pembrolizumab. We found that the two novel TRs showed similar binding affinity to the targets with respect to validated mAbs, even though they recognized distinct or only partially overlapping epitopes. When tested for their functional properties, they showed an increased ability to induce lymphocyte activation and stronger in vitro cytotoxicity against tumor cells compared to combinatorial treatments of clinically validated mAbs. Considering that tribodies also have other advantages with respect to combinatorial treatments, such as reduced production costs and lower dose requirements, we think that these novel immunomodulatory TRs could be used for therapeutic applications, particularly in monotherapy-resistant cancer patients.
The Usage of Cryopreserved Reproductive Material in Cancer Patients Undergoing Fertility Preservation Procedures
Authors: Wnuk Katarzyna , Świtalski Jakub , Miazga Wojciech , Tatara Tomasz , Religioni Urszula , Olszewski Paweł , Augustynowicz Anna
Published: November 2023

Background: Many cancer treatment methods can affect fertility by damaging the reproductive organs and glands that control fertility. Changes can be temporary or permanent. In order to preserve the fertility of cancer patients and protect the genital organs against gonadotoxicity, methods of fertility preservation are increasingly used. Considering that some patients ultimately decide not to use cryopreserved reproductive material, this review analysed the percentage of post-cancer patients using cryopreserved reproductive material, collected before treatment as part of fertility preservation. Methods: A systematic search of studies was carried out in accordance with the Cochrane Collaboration guidelines, based on a previously prepared research protocol. The search was conducted in Medline (via PubMed), Embase (via OVID), and the Cochrane Library. In addition, a manual search was performed for recommendations/clinical practice guidelines regarding fertility preservation in cancer patients. Results: Twenty-six studies met the inclusion criteria. The studies included in the review discussed the results of cryopreservation of oocytes, embryos, ovarian tissue, and semen. In 10 studies, the usage rate of cryopreserved semen ranged from 2.6% to 21.5%. In the case of cryopreserved female reproductive material, the return/usage rate ranged from 3.1% to 8.7% for oocytes, approx. 9% to 22.4% for embryos, and 6.9% to 30.3% for ovarian tissue. In studies analysing patients’ decisions about unused reproductive material, continuation of material storage was most often indicated. Recovering fertility or death of the patient were the main reasons for rejecting cryopreserved semen in the case of men. Conclusion: Fertility preservation before gonadotoxic treatment is widely recommended and increasingly used in cancer patients. The usage rate is an important indicator for monitoring the efficacy of these methods. In all of the methods described in the literature, this indicator did not exceed 31%. It is necessary to create legal and organizational solutions regulating material collection and storage and to create clear paths for its usage in the future, including by other recipients.
Circular RNA from Tyrosylprotein Sulfotransferase 2 Gene Inhibits Cisplatin Sensitivity in Head and Neck Squamous Cell Carcinoma by Sponging miR-770-5p and Interacting with Nucleolin
Authors: Wang Tianqing , Xin Chuan , Zhang Shiyu , Tian Xin , Hu Yuting , Wang Ying , Wang Jiongke , Ji Ning , Zeng Xin , Li Jing
Published: November 2023

Chemoresistance poses a significant challenge in the treatment of advanced head and neck squamous cell cancer (HNSCC). The role and mechanism of circular RNAs (circRNAs) in HNSCC chemoresistance remain understudied. We conducted circRNA microarray analysis to identify differentially expressed circRNAs in HNSCC. The expression of circRNAs from the tyrosylprotein sulfotransferase 2 (TPST2) gene and miRNAs was evaluated through qPCR, while the circular structure of circTPST2 was verified using Sanger sequencing and RNase R. Through Western blotting, biotin-labeled RNA pulldown, RNA immunoprecipitation, mass spectrometry, and rescue experiments, we discovered miR-770-5p and nucleolin as downstream targets of circTPST2. Functional tests, including CCK8 assays and flow cytometry, assessed the chemoresistance ability of circTPST2, miR-770-5p, and Nucleolin. Additionally, FISH assays determined the subcellular localization of circTPST2, miR-770-5p, and Nucleolin. IHC staining was employed to detect circTPST2 and Nucleolin expression in HNSCC patients. circTPST2 expression was inversely correlated with cisplatin sensitivity in HNSCC cell lines. Remarkably, high circTPST2 expression correlated with lower overall survival rates in chemotherapeutic HNSCC patients. Mechanistically, circTPST2 reduced chemosensitivity through sponge-like adsorption of miR-770-5p and upregulation of the downstream protein Nucleolin in HNSCC cells. The TCGA database revealed improved prognosis for patients with low circTPST2 expression after chemotherapy. Moreover, analysis of HNSCC cohorts demonstrated better prognosis for patients with low Nucleolin protein expression after chemotherapy. We unveil circTPST2 as a circRNA associated with chemoresistance in HNSCC, suggesting its potential as a marker for selecting chemotherapy regimens in HNSCC patients. Further exploration of the downstream targets of circTPST2 advanced our understanding and improved treatment strategies for HNSCC.
Cornerstone Cellular Pathways for Metabolic Disorders and Diabetes Mellitus: Non-Coding RNAs, Wnt Signaling, and AMPK
Authors: Maiese Kenneth
Published: November 2023

Metabolic disorders and diabetes (DM) impact more than five hundred million individuals throughout the world and are insidious in onset, chronic in nature, and yield significant disability and death. Current therapies that address nutritional status, weight management, and pharmacological options may delay disability but cannot alter disease course or functional organ loss, such as dementia and degeneration of systemic bodily functions. Underlying these challenges are the onset of aging disorders associated with increased lifespan, telomere dysfunction, and oxidative stress generation that lead to multi-system dysfunction. These significant hurdles point to the urgent need to address underlying disease mechanisms with innovative applications. New treatment strategies involve non-coding RNA pathways with microRNAs (miRNAs) and circular ribonucleic acids (circRNAs), Wnt signaling, and Wnt1 inducible signaling pathway protein 1 (WISP1) that are dependent upon programmed cell death pathways, cellular metabolic pathways with AMP-activated protein kinase (AMPK) and nicotinamide, and growth factor applications. Non-coding RNAs, Wnt signaling, and AMPK are cornerstone mechanisms for overseeing complex metabolic pathways that offer innovative treatment avenues for metabolic disease and DM but will necessitate continued appreciation of the ability of each of these cellular mechanisms to independently and in unison influence clinical outcome.
Evaluation of the Wear Resistance and Electrochemical Behavior of Drill Bits for Drilling Oil and Gas Wells
Authors: Hincapie-Campos Williams Steve , Avellaneda Daniel , Carrillo-Álvarez Víctor Manuel , Olaya Jhon Jairo
Published: November 2023

This work aims to study the relation between the hardness and behavioral wear of tungsten carbide inserts (TCI) and polycrystalline diamond compact (PDC) cutters, used as drill bits for drilling oil and gas wells, through wear testing conducted using the pin-on-disc test and the measure of microhardness Knoop test. The morphology of the worn surface was characterized using scanning electron microscopy (SEM), and the chemical composition was taken before and after the wear test inside the tracks using energy-dispersive X-ray spectrometry (EDX). Additionally, the behavior of the corrosion resistance was evaluated with the electrodynamic polarization technique and was compared with a coating of diamond-like carbon (DLC) in order to determine whether this coating applied to tool steel could serve as a substitute for insert-based coatings in future applications, as its cost is lower than that of the inserts. A paragraph discusses what characteristics must be taken when selecting or manufacturing materials for drill bit applications. In conclusion, it was found that the TCI insert presented cracks in the test tracks, while the PDC insert showed soft wear; this was due to the uncertain hardness, which was greater than the indenter used. Concerning the Tafel test, the curves showed that the PDC insert presents better behavior under corrosion; however, the DLC can be a good substitute.
Fabrication and Characterization of TiO2 Coatings on 304 Stainless-Steel Substrate for Efficient Oil/Water Separation
Authors: Sico Jose , Tang Benjamin , Flores Dayana , Mouawad Roy , Punsalan Rheyana , Gan Yong X. , Li Mingheng
Published: November 2023

Oil spill accidents have been a prevalent threat to the environment. To aid in clean-up efforts, a stainless-steel filter with a hydrophilic and oleophobic coating was fabricated for efficient and affordable oil/water separation. Two solutions were used to deposit the coatings. One was sourced from a titanium (IV) isopropoxide (TTIP) precursor dissolved into 1-butanol and the other through the mixing of titanium dioxide nanopowder with glacial acetic acid. The solutions were applied to 304 stainless-steel mesh filters of varying aperture sizes ranging from 30 microns to 240 microns. The coating was applied through a multiphase deposition method followed by sintering at 450 °C. The filter performance was evaluated by contact angle measurement and a filtration test using a mixture of motor oil and water, while the surface morphology and structure of the coatings were characterized by SEM-EDS and XRD. The mesh with smaller aperture size showed oil retention improvement of up to 99%. The TiO2 nanopowder coating, with a 92% oil retention efficiency, outperformed the coating via the TTIP precursor.
Antibacterial Calcium Phosphate Coatings with Zinc Oxide Nanoparticles
Authors: Chebodaeva Valentina , Sedelnikova Mariya , Khimich Margarita , Bakina Olga , Tolmachev Alexey , Miller Andrey , Golohvast Kirill , Zakharenko Aleksander , Egorkin Vladimir , Vyaliy Igor , Sharkeev Yurii
Published: November 2023

Porous calcium phosphate coatings (C-P) with ZnO nanoparticles were obtained via the micro-arc oxidation method on a titanium substrate. ZnO nanoparticles were added to the C-P coatings to change the zeta potential and improve the coatings’ bioactivity and antibacterial properties. The samples with coatings were studied via scanning electron microscopy (SEM), X-ray diffraction, energy dispersive microanalysis, potentiodynamic polarization, and zeta potential measurement. The coatings modified with ZnO nanoparticles showed improved physical, electrochemical, and electrical properties, compared to the initial unmodified coatings. Modification with ZnO nanoparticles contributed to an increase in zeta potential from −60 mV to −53 mV. Functionalization of the coatings with ZnO nanoparticles allowed us to increase the anticorrosion characteristics by about 30%. The biological studies showed that the coatings had no cytotoxic effect on L929 fibroblast cells. The antibacterial activity of the coating rose by 99% after the addition of ZnO nanoparticles against the bacterium Staphylococcus aureus.
Green Synthesis of Nanoparticles and Their Energy Storage, Environmental, and Biomedical Applications
Authors: Abuzeid Hanaa M. , Julien Christian M. , Zhu Likun , Hashem Ahmed M.
Published: November 2023

Green synthesis offers a superior alternative to traditional methods for producing metal and metal oxide nanoparticles. This approach is not only benign and safe but also cost-effective, scalable, and straightforward, operating under ambient conditions. Notable metals and metal oxide nanoparticles, such as manganese oxides, iron oxides, silver, and gold, have been produced using various bio-reductants derived from plant extracts. These biological agents not only expedite the reduction process but also stabilize the nanoparticles, serving dual roles as reducing and capping agents. This review presents the green synthesis of nanoparticles (NPs) obtained from biogenic wastes and plant extracts. The green-synthesized nanostructured MnO2 nanoparticles are evaluated as a potential photocatalyst for water treatment and as an electrode material in lithium-ion batteries and supercapacitors. The green-derived iron oxide nanoparticles are examined as promising antioxidant, anti-inflammatory, and anti-diabetic agents. Additionally, this review discusses the green synthesis of precious metal nanoparticles, specifically silver (Ag NPs) and gold (Au NPs), highlighting their potential medical applications in areas like antiviral treatments and cancer therapy.
Innovative Method for the Mass Preparation of α″-Fe16N2 Powders via Gas Atomization
Authors: Grigoras Marian , Lostun Mihaela , Porcescu Marieta , Stoian George , Ababei Gabriel , Lupu Nicoleta
Published: November 2023

The iron nitride materials, especially α″-Fe16N2, are considered one of the most promising candidates for future rare-earth-free magnets. However, the mass production of α″-Fe16N2 powders as a raw material for permanent magnets is still challenging. In this work, starting from iron lumps as a raw material, we have managed to prepare the α″-Fe16N2 powders via the gas atomization method, followed by subsequent nitriding in an ammonia–hydrogen gas mixture stream. The particle size was controlled by changing the gas atomization preparation conditions. X-ray diffractograms (XRD) analyses show that the prepared powders are composed of α″-Fe16N2 and α-Fe phases. The α″-Fe16N2 volume ratio increases with decreasing powder size and increasing nitriding time, reaching a maximum of 57% α″-Fe16N2 phase in powders with size below 32 ± 3 μm after 96 h nitridation. The saturation magnetization reaches the value of 237 emu/g and a reasonable coercivity value of 884 Oe. Compared to the saturation magnetization values of α-Fe powders, the α″-Fe16N2 powders prepared through our proposed approach show an increase of up to 10% in saturation and demonstrate the possibility of mass production of α″-Fe16N2 powders as precursors of permanent magnets without rare earths.
Prevalence and Genetic Characterization of Morphologically Indistinguishable Sarcocysts of Sarcocystis cruzi in Cattle and Sarcocystis poephagicanis in Yaks
Authors: Tang Kui , Lamu Danqu , Qin Tao , Liao Zhe , Zhang Mingzhu , Wu Zhipeng , Deng Shuangsheng , Tao Jianping , Hu Junjie
Published: November 2023

Sarcocystis cruzi in cattle (Bos taurus) and Sarcocystis poephagicanis in yaks (Bos grunniens) are morphologically indistinguishable. However, the relationship between the two parasites is still unclear. Here, muscular tissues of the two species of domestic animal collected from abattoirs in China were examined for sarcocysts of S. cruzi and S. poephagicanis. The sarcocysts isolated from the samples were analyzed using light microscopy (LM), transmission electron microscopy (TEM), and DNA analysis. Sarcocysts of S. cruzi and S. poephagicanis were found in 405 of 950 (42.6%) cattle and 304 of 320 (95.0%) yaks. LM and TEM showed that the sarcocysts of the two parasites had similar morphological characteristics. The thin-walled sarcocysts had hair-like protrusions on the surface. The ultrastructures were demonstrated to include a primary cyst wall containing irregularly folded, hirsute, or bone-like protrusions. Four genetic markers of the two parasites were sequenced and analyzed, namely, 18S rDNA, 28S rDNA, mitochondrial cox1, and apicoplast rpl6. The sequences of the four loci had an interspecific similarity of 97.9–98.6%, 97.2–98.1%, 89.5–90.4%, and 96.9–97.2% identity, respectively. Phylogenetic analysis using 28S rDNA and cox1 sequences indicated that both S. cruzi and S. poephagicanis were placed into a group encompassing Sarcocystis spp. in ruminants with canid as known or putative definitive hosts. Sarcocystis cruzi and S. poephagicanis represent separate species, and cox1 and rpl6 were suitable for distinguishing between them.
Evaluation of the BD Phoenix Carbapenemase-Producing Organism Panels for the Detection of Carbapenemase Producers in Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa
Authors: Correa-León Yoselin Paola , Pérez-Hernández José Miguel , Martinez-Guerra Bernardo Alfonso , Rodríguez-Noriega Eduardo , Mena-Ramírez Juan Pablo , López-Gutiérrez Eduardo , López-Jácome Luis Esaú , Monroy-Colin Víctor Antonio , Mireles-Davalos Christian Daniel , Padilla-Ibarra Cecilia , Quevedo-Ramos María Angelina , Feliciano-Guzmán José Manuel , Pérez-Vicelis Talía , Velázquez-Acosta María del Consuelo , Hernández-Durán Melissa , Garza-González Elvira
Published: November 2023

The classification of carbapenemases can help guide therapy. The present study evaluated the performance of the CPO detection test, included in the BD Phoenix™ NMIC-501 panel for the detection and classification of carbapenemases on the representative molecularly characterized strains collection from Mexico. Carbapenem non-susceptible isolates collected in Mexico were included. The clinical isolates (n = 484) comprised Klebsiella pneumoniae (n = 154), Escherichia coli (n = 150), and P. aeruginosa (n = 180). BD Phoenix CPO NMIC-504 and NMIC-501 panels were used for the identification of species, antimicrobial susceptibility tests, and detection of CPOs. For the detection of carbapenemase-encoding genes, E. coli and K. pneumoniae were evaluated using PCR assays for blaNDM-1, blaKPC, blaVIM, blaIMP, and blaOXA-48-like. For P. aeruginosa, blaVIM, blaIMP, and blaGES were detected using PCR. Regarding E. coli, the CPO panels had a sensitivity of 70% and specificity of 83.33% for the detection of a class B carbapenemase (blaNDM in the molecular test). Regarding K. pneumoniae, the panels had a sensitivity of 75% and specificity of 100% for the detection of a class A carbapenemase (blaKPC in the molecular test). The Phoenix NMIC-501 panels are reliable for detecting class B carbapenemases in E. coli. The carbapenemase classification in K. pneumoniae for class A carbapenemases has a high specificity and PPV; thus, a positive result is of high value.
Association of COVID-19 Infection with Sociodemographic, Anthropometric and Lifestyle Factors: A Cross-Sectional Study in an Older Adults’ Population Aged over 65 Years Old
Authors: Pavlidou Eleni , Papadopoulou Sousana K. , Antasouras Georgios , Vorvolakos Theofanis , Alexatou Olga , Tsourouflis Gerasimos , Angelakou Exakousti-Petroula , Serdari Aspasia , Grammatikopoulou Maria G. , Psara Evmorfia , Vadikolias Konstantinos , Dakanalis Antonios , Lefantzis Nikolaos , Giaginis Constantinos
Published: November 2023

Background: The COVID-19 pandemic has led to unfavorable disruptions to daily living routines by exerting deleterious effects on several aspects of human mental and physical health and quality of life worldwide. The purpose of the current survey is to explore the potential association of COVID-19 infection with multiple sociodemographic, anthropometric, and lifestyle factors of community-dwelling older adults. Methods: This is a cross-sectional survey including 5197 older adults aged over 65 years old from 10 geographically diverse regions of Greece. Relevant questionnaires were used to record study the population sociodemographic factor, while anthropometric parameters were also measured. Validated questionnaires were also applied to assess several lifestyle factors such as depression, anxiety, stress, cognitive status, sleep quality, health-related quality of life, physical activity levels, and Mediterranean diet (MD) adherence. Results: In multivariate regression analysis, COVID-19 infection was significantly, independently related with urban residence (p = 0.0107), regular smoking (p = 0.0218), overweight status and obesity (p = 0.0036), as well as abdominal obesity (p = 0.0008), higher risk of depression (p = 0.0027), anxiety (p = 0.0045), stress (p = 0.0038), inadequate sleep quality (p = 0.0108), lower physical activity levels (p = 0.0012), reduced MD compliance (p = 0.0009), and poor health-related quality of life (p = 0.0002). In univariate analysis, older adults’ age (p = 0.0001), male gender (p = 0.0015), living alone (p = 0.0023), lower educational and economic level (p = 0.0175 and p = 0.0294, respectively), and cognition decline (p = 0.0032) were also related with the presence of COVID-19 infection; however, these associations were considerably attenuated at a non-significant level by adjustment for several confounders in multivariate analysis. Conclusions: This is one of the few available studies supporting evidence that COVID-19 infection may be associated with diverse sociodemographic, anthropometric, and lifestyle factors in an older adults’ population in Greece. This study highlights the strong demand to provide psychological and nutritional counselling and support to older adults diagnosed with COVID-19 infection in order to ameliorate disease symptoms and severity, emphasizing the adaptation of healthy dietary and lifestyle habits as preventing and supplementary therapeutic factors against COVID-19.
Using UAVs and Machine Learning for Nothofagus alessandrii Species Identification in Mediterranean Forests
Authors: Cabrera-Ariza Antonio M. , Peralta-Aguilera Miguel , Henríquez-Hernández Paula V. , Santelices-Moya Rómulo
Published: November 2023

This study explores the use of unmanned aerial vehicles (UAVs) and machine learning algorithms for the identification of Nothofagus alessandrii (ruil) species in the Mediterranean forests of Chile. The endangered nature of this species, coupled with habitat loss and environmental stressors, necessitates efficient monitoring and conservation efforts. UAVs equipped with high-resolution sensors capture orthophotos, enabling the development of classification models using supervised machine learning techniques. Three classification algorithms—Random Forest (RF), Support Vector Machine (SVM), and Maximum Likelihood (ML)—are evaluated, both at the Pixel- and Object-Based levels, across three study areas. The results reveal that RF consistently demonstrates strong classification performance, followed by SVM and ML. The choice of algorithm and training approach significantly impacts the outcomes, highlighting the importance of tailored selection based on project requirements. These findings contribute to enhancing species identification accuracy in remote sensing applications, supporting biodiversity conservation and ecological research efforts.
Vehicle-to-Vehicle Based Autonomous Flight Coordination Control System for Safer Operation of Unmanned Aerial Vehicles
Authors: Shan Lin , Miura Ryu , Matsuda Takashi , Koshikawa Miho , Li Huan-Bang , Matsumura Takeshi
Published: November 2023

The exponential growth of unmanned aerial vehicles (UAVs) or drones in recent years has raised concerns about their safe operation, especially in beyond-line-of-sight (BLOS) scenarios. Existing unmanned aircraft system traffic management (UTM) heavily relies on commercial communication networks, which may become ineffective if network infrastructures are damaged or disabled. For this challenge, we propose a novel approach that leverages vehicle-to-vehicle (V2V) communications to enhance UAV safety and efficiency in UAV operations. In this study, we present a UAV information collection and sharing system named Drone Mapper®, enabled by V2V communications, so that UAVs can share their locations with each another as well as with the ground operation station. Additionally, we introduce an autonomous flight coordination control system (AFCCS) that augments UAV safety operations by providing two essential functionalities: UAV collision avoidance and UAV formation flight, both of which work based on V2V communications. To evaluate the performance of the developed AFCCS, we conducted comprehensive field experiments focusing on UAV collision avoidance and formation flight. The experimental results demonstrate the effectiveness of the proposed system and show seamless operations among multiple UAVs.