bluuebunny/crossref_metadata_2025 huggingface dataset:
Intraperitoneal Paclitaxel Treatment for Patients with Pancreatic Ductal Adenocarcinoma with Peritoneal Dissemination Provides a Survival Benefit
Authors: Yamamoto Tomohisa , Satoi Sohei , Yamaki So , Hashimoto Daisuke , Ishida Mitsuaki , Ikeura Tsukasa , Hirooka Satoshi , Matsui Yuki , Boku Shogen , Nakayama Shinji , Nakamaru Koh , Shibata Nobuhiro , Katsushima Utae , Sekimoto Mitsugu
Published: March 2022

Background: Intraperitoneal chemotherapy using paclitaxel (i.p.-PTX) is expected to be a new therapeutic strategy for patients with pancreatic ductal adenocarcinoma (PDAC) and peritoneal dissemination. We evaluated the survival benefit of i.p.-PTX compared with standard systemic chemotherapy. Methods: Clinical data of 101 consecutive PDAC patients with peritoneal dissemination between 2007 and 2018 were analyzed. All patients were determined to have no other sites of distant organ metastasis to the lung, bone, or liver on contrast-enhanced CT imaging. Patients underwent staging laparoscopy or open laparotomy to confirm pathological evidence of peritoneal dissemination, and to exclude occult liver metastasis. Survival curves were estimated using the Kaplan–Meier method, and differences were compared using the log-rank test. Results: Forty-three patients were treated with i.p.-PTX (i.p.-PTX group) and forty-nine patients received standard systemic chemotherapy (Ctrl group). Nine patients did not receive any treatment (BSC group). The median survival time (MST) in the i.p.-PTX group was significantly longer than that in the Ctrl group (17.9 months vs. 10.2 months, p = 0.006). Negative peritoneal washing cytology was observed in 24 out of 43 patients in the i.p.-PTX group. The i.p.-PTX group tended to have a higher proportion of clinical responses than the Ctrl group (30% vs. 18%, p = 0.183). Conversion surgery was performed in 10 patients in the i.p.-PTX group and 2 patients in the Ctrl group after confirming disappearance of peritoneal dissemination with staging laparoscopy or open laparotomy (p = 0.005). The MST in patients who underwent surgical resection was significantly longer than that in patients who did not (27.4 months vs. 11.3 months; p < 0.0001). Conclusion: i.p.-PTX therapy provided improved survival in PDAC patients with peritoneal dissemination, and conversion surgery enhanced it in patients with favorable responses to chemotherapy. i.p.-PTX might become one of the treatment options to PDAC patients with peritoneal dissemination.
Three-Dimensional Graphene with Preserved Channeling as a Binder Additive for Zeolite 13X for Enhanced Thermal Conductivity, Vapor Transport, and Vapor Adsorption Loading Kinetics
Authors: Gildernew Evan , Tareq Syed , Yang Sungwoo
Published: March 2022

Atmospheric water vapor extraction through adsorption to highly porous materials holds promise for its incorporation into broader technologies, including potable water generation. These technologies require breakthroughs in synthesis and design. Here, we demonstrate a composite of zeolite 13X sorbent for high adsorption capacity infiltrated with a light-weight three-dimensional graphene binder, which effectively networks a substrate structure into the sorbent. The composites described maintained fidelity when passing through the pore structure. This was accomplished by the utilization of a sacrificial polymer for safeguarding channel networking during sorbent infiltration of the binder for the extension of substrate networking. The performance measures for adsorbate loadings and thermal flux are evaluated with additional measurements taken for considering compactions of sorbent/substrates. Graphene/Zeolite 13X with preserved channeling demonstrated specific heat flux at 7664 W/kg, while samples without preserved channeling measured 4206 W/kg. A 0.6 g/cm3 compaction resulted in a 412% and a 368% improvement in mass transport while compaction at 1.2 g/cm3 resulted in a 333% and a 290% improvement in mass transport.
Preparation of Cordierite Monolith Catalysts with the Coating of K-Modified Spinel MnCo2O4 Oxide and Their Catalytic Performances for Soot Combustion
Authors: Zhao Kun , Li Jianmei , Wang Lanyi , Li Dong , Liu Bonan , Li Renjie , Yu Xuehua , Wei Yuechang , Liu Jian , Zhao Zhen
Published: March 2022

Diesel engines are important for heavy-duty vehicles. However, particulate matter (PM) released from diesel exhaust should be eliminated. Nowadays, catalytic diesel particulate filters (CDPF) are recognized as a promising technology. In this work, a series of monolith Mn1−nKnCo2O4 catalysts were prepared by the simple citric acid method. The as-prepared catalysts displayed good catalytic performance for soot combustion and the Mn0.7K0.3Co2O4 catalyst gave the best catalytic performance among all the prepared samples. The T10 and Tm of Mn0.7K0.3Co2O4-HC catalyst for soot combustion are 310 and 439 °C, respectively. The physical and chemical properties of catalysts were characterized by means of SEM, XPS, H2-TPR, Raman and other techniques. The characterization results indicate that K substitution is favorable for the formation of oxygen vacancies, enhancing the mobility of active oxygen species, and improving the redox properties and so on. In-situ Raman results prove that the strength of Co-O bonds in the catalysts became weak during the reaction at high temperatures. In addition, SEM and ultrasonic test results show that the peeling rate of the coat-layer is less than 5%. The as-prepared catalysts can be taken as one kind of candidate catalyst for promising application in soot combustion because of its facile synthesis, low cost and high catalytic activity.
A Mixed Heterobimetallic Y/Eu-MOF for the Cyanosilylation and Hydroboration of Carbonyls
Authors: Echenique-Errandonea Estitxu , López-Vargas Mireya E. , Pérez Juana M. , Rojas Sara , Choquesillo-Lazarte Duane , Seco José M. , Fernández Ignacio , Rodríguez-Diéguez Antonio
Published: March 2022

Herein, to the best of our knowledge, the first heterobimetallic Y/Eu porous metal–organic framework (MOF), based on 3-amino-4-hydroxybenzoic acid (H2L) ligand, with the following formulae {[Y3.5Eu1.5L6(OH)3(H2O)3]·12DMF}n (in advance, namely Y/Eu-MOF), is described. The three-dimensional structure has been synthesized by solvothermal routes and thoroughly characterized, by means of single crystal X-ray diffraction, powder X-ray diffraction, electronic microscopy, ICP-AES, electrophoretic mobility, and FTIR spectra. Intriguingly, the porous nature allows for coordinated solvent molecules displacement, yielding unsaturated metal centers, which can act as a Lewis acid catalyst. This novel supramolecular entity has been tested in cyanosilylation and hydroboration reactions on carbonyl substrates of a diverse nature, exhibiting an extraordinary activity.
Metal Oxides as Catalyst/Supporter for CO2 Capture and Conversion, Review
Authors: Khdary Nezar H. , Alayyar Alhanouf S. , Alsarhan Latifah M. , Alshihri Saeed , Mokhtar Mohamed
Published: March 2022

Various carbon dioxide (CO2) capture materials and processes have been developed in recent years. The absorption-based capturing process is the most significant among other processes, which is widely recognized because of its effectiveness. CO2 can be used as a feedstock for the production of valuable chemicals, which will assist in alleviating the issues caused by excessive CO2 levels in the atmosphere. However, the interaction of carbon dioxide with other substances is laborious because carbon dioxide is dynamically relatively stable. Therefore, there is a need to develop types of catalysts that can break the bond in CO2 and thus be used as feedstock to produce materials of economic value. Metal oxide-based processes that convert carbon dioxide into other compounds have recently attracted attention. Metal oxides play a pivotal role in CO2 hydrogenation, as they provide additional advantages, such as selectivity and energy efficiency. This review provides an overview of the types of metal oxides and their use for carbon dioxide adsorption and conversion applications, allowing researchers to take advantage of this information in order to develop new catalysts or methods for preparing catalysts to obtain materials of economic value.
Insight into Crystallization Features of MOR Zeolite Synthesized via Ice-Templating Method
Authors: Zhao Shangqing , Li Haiwei , Zhang Weilong , Wang Bo , Yang Xiaolong , Peng Yanhua , Zhang Yan , Li Zhuo
Published: March 2022

Hydrothermal, solvothermal or ionothermal routes are usually employed for the synthesis of zeolite, which is often accompanied by a high energy consumption, high cost and low efficiency. We have developed a novel route for the rapid and high yield synthesis of mordenite (MOR) zeolite via an ice-templating method. In comparison with traditional hydrothermal synthesis, not only the high yield, but also the superior crystallinity, large reduction in water level and reaction pressure, simple device and conventional silica sources by this route can have great potential for the commercial production of pure MOR zeolite. Moreover, the changed bonding environment of silicon atoms in MOR zeolite, that is, a relative decrease in the tetrahedrally coordinated Si–O–Si bond, and accordingly, an increase in the T–OH (T = Si, Al) groups and Si–O–Al sites, remarkably enhances its acid strength.
Dynamics of T-Cell Intracellular Antigen 1-Dependent Stress Granules in Proteostasis and Welander Distal Myopathy under Oxidative Stress
Authors: Fernández-Gómez Andrea , Velasco Beatriz , Izquierdo José
Published: March 2022

T-cell intracellular antigen 1 (TIA1) is an RNA-binding protein that is primarily involved in the post-transcriptional regulation of cellular RNAs. Furthermore, it is a key component of stress granules (SGs), RNA, and protein aggregates that are formed in response to stressful stimuli to reduce cellular activity as a survival mechanism. TIA1 p.E384K mutation is the genetic cause of Welander distal myopathy (WDM), a late-onset muscular dystrophy whose pathogenesis has been related to modifying SG dynamics. In this study, we present the results obtained by analyzing two specific aspects: (i) SGs properties and dynamics depending on the amino acid at position 384 of TIA1; and (ii) the formation/disassembly time-course of TIA1WT/WDM-dependent SGs under oxidative stress. The generation of TIA1 variants—in which the amino acid mutated in WDM and the adjacent ones were replaced by lysines, glutamic acids, or alanines—allowed us to verify that the inclusion of a single lysine is necessary and sufficient to alter SGs dynamics. Moreover, time-lapse microscopy analysis allowed us to establish in vivo the dynamics of TIA1WT/WDM-dependent SG formation and disassembly, after the elimination of the oxidizing agent, for 1 and 3 h, respectively. Our observations show distinct dynamics between the formation and disassembly of TIA1WT/WDM-dependent SGs. Taken together, this study has allowed us to expand the existing knowledge on the role of TIA1 and the WDM mutation in SG formation.
Lysosomal ATP Transporter SLC17A9 Controls Cell Viability via Regulating Cathepsin D
Authors: Huang Peng , Cao Qi , Xu Mengnan , Dong Xian-Ping
Published: March 2022

SLC17A9 (solute carrier family 17 member 9) functions as an ATP transporter in lysosomes as well as other secretory vesicles. SLC17A9 inhibition or silence leads to cell death. However, the molecular mechanisms causing cell death are unclear. In this study, we report that cell death induced by SLC17A9 deficiency is rescued by the transcription factor EB (TFEB), a master gene for lysosomal protein expression, suggesting that SLC17A9 deficiency may be the main cause of lysosome dysfunction, subsequently leading to cell death. Interestingly, Cathepsin D, a lysosomal aspartic protease, is inhibited by SLC17A9 deficiency. Heterologous expression of Cathepsin D successfully rescues lysosomal dysfunction and cell death induced by SLC17A9 deficiency. On the other hand, the activity of Cathepsin B, a lysosomal cysteine protease, is not altered by SLC17A9 deficiency, and Cathepsin B overexpression does not rescue lysosomal dysfunction and cell death induced by SLC17A9 deficiency. Our data suggest that lysosomal ATP and SLC17A9 play critical roles in lysosomal function and cell viability by regulating Cathepsin D activity.
Polyamine Immunometabolism: Central Regulators of Inflammation, Cancer and Autoimmunity
Authors: Chia Tzu-yi , Zolp Andrew , Miska Jason
Published: March 2022

Polyamines are ubiquitous, amine-rich molecules with diverse processes in biology. Recent work has highlighted that polyamines exert profound roles on the mammalian immune system, particularly inflammation and cancer. The mechanisms by which they control immunity are still being described. In the context of inflammation and autoimmunity, polyamine levels inversely correlate to autoimmune phenotypes, with lower polyamine levels associated with higher inflammatory responses. Conversely, in the context of cancer, polyamines and polyamine biosynthetic genes positively correlate with the severity of malignancy. Blockade of polyamine metabolism in cancer results in reduced tumor growth, and the effects appear to be mediated by an increase in T-cell infiltration and a pro-inflammatory phenotype of macrophages. These studies suggest that polyamine depletion leads to inflammation and that polyamine enrichment potentiates myeloid cell immune suppression. Indeed, combinatorial treatment with polyamine blockade and immunotherapy has shown efficacy in pre-clinical models of cancer. Considering the efficacy of immunotherapies is linked to autoimmune sequelae in humans, termed immune-adverse related events (iAREs), this suggests that polyamine levels may govern the inflammatory response to immunotherapies. This review proposes that polyamine metabolism acts to balance autoimmune inflammation and anti-tumor immunity and that polyamine levels can be used to monitor immune responses and responsiveness to immunotherapy.
Metabolomic Profiling and Mechanotransduction of Single Chondrocytes Encapsulated in Alginate Microgels
Authors: Fredrikson Jacob P. , Brahmachary Priyanka P. , Erdoğan Ayten E. , Archambault Zachary K. , Wilking James N. , June Ronald K. , Chang Connie B.
Published: March 2022

Articular cartilage is comprised of two main components, the extracellular matrix (ECM) and the pericellular matrix (PCM). The PCM helps to protect chondrocytes in the cartilage from mechanical loads, but in patients with osteoarthritis, the PCM is weakened, resulting in increased chondrocyte stress. As chondrocytes are responsible for matrix synthesis and maintenance, it is important to understand how mechanical loads affect the cellular responses of chondrocytes. Many studies have examined chondrocyte responses to in vitro mechanical loading by embedding chondrocytes in 3-D hydrogels. However, these experiments are mostly performed in the absence of PCM, which may obscure important responses to mechanotransduction. Here, drop-based microfluidics is used to culture single chondrocytes in alginate microgels for cell-directed PCM synthesis that closely mimics the in vivo microenvironment. Chondrocytes formed PCM over 10 days in these single-cell 3-D microenvironments. Mechanotransduction studies were performed, in which single-cell microgels mimicking the cartilage PCM were embedded in high-stiffness agarose. After physiological dynamic compression in a custom-built bioreactor, microgels exhibited distinct metabolomic profiles from both uncompressed and monolayer controls. These results demonstrate the potential of single cell encapsulation in alginate microgels to advance cartilage tissue engineering and basic chondrocyte mechanobiology.
Evaluation of Immunoregulatory Biomarkers on Plasma Small Extracellular Vesicles for Disease Progression and Early Therapeutic Response in Head and Neck Cancer
Authors: Jablonska Jadwiga , Rist Malwina , Spyra Ilona , Tengler Luisa , Domnich Maksim , Kansy Benjamin , Giebel Bernd , Thakur Basant Kumar , Rotter Nicole , Lang Stephan , Ludwig Sonja
Published: March 2022

Head and Neck Cancers (HNCs) have highly immunosuppressive properties. Small extracellular vesicles (sEVs), including exosomes, nanosized mediators of intercellular communication in the blood, carry immunosuppressive proteins and effectively inhibit anti-tumor immune responses in HNCs. This study evaluates immunosuppressive markers on sEVs from 40 HNC patients at different disease stages and 3- and 6-month follow-up after surgery and/or chemoradiotherapy. As controls, sEVs from normal donors (NDs) are examined. Immunoregulatory surface markers on sEVs were detected as relative fluorescence intensity (RFI) using on-bead flow cytometry, and their expression levels were monitored in the early and late stages of HNC and during follow-up. In parallel, the sEV-mediated apoptosis of CD8+ Jurkat cells was assessed. Together with TGF-β1 and PD-L1 abundance, total sEV proteins are elevated with disease progression. In contrast, total sEV protein, including TGF-β1, PD-1 and PD-L1, decrease upon therapy response during follow-up. Overall survival analysis implies that high sEV PD-1/PD-L1 content is an unfavorable prognostic marker in HNC. Consistently, the sEV-mediated induction of apoptosis in CD8+ T cells correlates with the disease activity and therapy response. These findings indicate that a combination of immunoregulatory marker profiles should be preferred over a single marker to monitor disease progression and therapy response in HNC.
Altered Plasma Fatty Acid Abundance Is Associated with Cachexia in Treatment-Naïve Pancreatic Cancer
Authors: Gumpper-Fedus Kristyn , Hart Phil A. , Belury Martha A. , Crowe Olivia , Cole Rachel M. , Pita Grisanti Valentina , Badi Niharika , Liva Sophia , Hinton Alice , Coss Christopher , Ramsey Mitchell L. , Noonan Anne , Conwell Darwin L. , Cruz-Monserrate Zobeida
Published: March 2022

Cachexia occurs in up to 80% of pancreatic ductal adenocarcinoma (PDAC) patients and is characterized by unintentional weight loss and tissue wasting. To understand the metabolic changes that occur in PDAC-associated cachexia, we compared the abundance of plasma fatty acids (FAs), measured by gas chromatography, of subjects with treatment-naïve metastatic PDAC with or without cachexia, defined as a loss of > 2% weight and evidence of sarcopenia (n = 43). The abundance of saturated, monounsaturated, and polyunsaturated FAs was not different between subjects with cachexia and those without. Oleic acid was significantly higher in subjects with cachexia (p = 0.0007) and diabetes (p = 0.015). Lauric (r = 0.592, p = 0.0096) and eicosapentaenoic (r = 0.564, p = 0.015) acids were positively correlated with age in cachexia patients. Subjects with diabetes (p = 0.021) or both diabetes and cachexia (p = 0.092) had low palmitic:oleic acid ratios. Linoleic acid was lower in subjects with diabetes (p = 0.018) and correlated with hemoglobin (r = 0.519, p = 0.033) and albumin (r = 0.577, p = 0.015) in subjects with cachexia. Oleic or linoleic acid may be useful treatment targets or biomarkers of cachexia in patients with metastatic PDAC, particularly those with diabetes.
Evaluation of Neurofilament Light Chain as a Biomarker of Neurodegeneration in X-Linked Childhood Cerebral Adrenoleukodystrophy
Authors: Wang Hongge , Davison Matthew D. , Kramer Martin L. , Qiu Weiliang , Gladysheva Tatiana , Chiang Ruby M. S. , Kayatekin Can , Nascene David R. , Taghizadeh Leyla A. , King Carina J. , Nolan Erin E. , Gupta Ashish O. , Orchard Paul J. , Lund Troy C.
Published: March 2022

Cerebral adrenoleukodystrophy (CALD) is a devastating, demyelinating neuroinflammatory manifestation found in up to 40% of young males with an inherited mutation in ABCD1, the causative gene in adrenoleukodystrophy. The search for biomarkers which correlate to CALD disease burden and respond to intervention has long been sought after. We used the Olink Proximity Extension Assay (Uppsala, Sweden) to explore the cerebral spinal fluid (CSF) of young males with CALD followed by correlative analysis with plasma. Using the Target 96 Neuro Exploratory panel, we found that, of the five proteins significantly increased in CSF, only neurofilament light chain (NfL) showed a significant correlation between CSF and plasma levels. Young males with CALD had a 11.3-fold increase in plasma NfL compared with controls. Importantly, 9 of 11 young males with CALD who underwent HCT showed a mean decrease in plasma NfL of 50% at 1 year after HCT compared with pre-HCT levels. In conclusion, plasma NfL could be a great value in determining outcomes in CALD and should be scrutinized in future studies in patients prior to CALD development and after therapeutic intervention.
Into the Tissues: Extracellular Matrix and Its Artificial Substitutes: Cell Signalling Mechanisms
Authors: Bandzerewicz Aleksandra , Gadomska-Gajadhur Agnieszka
Published: March 2022

The existence of orderly structures, such as tissues and organs is made possible by cell adhesion, i.e., the process by which cells attach to neighbouring cells and a supporting substance in the form of the extracellular matrix. The extracellular matrix is a three-dimensional structure composed of collagens, elastin, and various proteoglycans and glycoproteins. It is a storehouse for multiple signalling factors. Cells are informed of their correct connection to the matrix via receptors. Tissue disruption often prevents the natural reconstitution of the matrix. The use of appropriate implants is then required. This review is a compilation of crucial information on the structural and functional features of the extracellular matrix and the complex mechanisms of cell–cell connectivity. The possibilities of regenerating damaged tissues using an artificial matrix substitute are described, detailing the host response to the implant. An important issue is the surface properties of such an implant and the possibilities of their modification.
LED Light-Induced ROS Differentially Regulates Focal Adhesion Kinase Activity in HaCaT Cell Viability
Authors: Kim Jun-Sub , Lim Ssang-Taek
Published: March 2022

In this study, changes in cell signaling mechanisms in skin cells induced by various wavelengths and intensities of light-emitting diodes (LED) were investigated, focusing on the activity of focal adhesion kinase (FAK) in particular. We examined the effect of LED irradiation on cell survival, the generation of intracellular reactive oxygen species (ROS), and the activity of various cell-signaling proteins. Red LED light increased cell viability at all intensities, whereas strong green and blue LED light reduced cell viability, and this effect was reversed by NAC or DPI treatment. Red LED light caused an increase in ROS formation according to the increase in the intensity of the LED light, and green and blue LED lights led to sharp increases in ROS formation. In the initial reaction to LEDs, red LED light only increased the phosphorylation of FAK and extracellular-signal regulated protein kinase (ERK), whereas green and blue LED lights increased the phosphorylation of inhibitory-κB Kinase α (IKKα), c-jun N-terminal kinase (JNK), and p38. The phosphorylation of these intracellular proteins was reduced via FAK inhibitor, NAC, and DPI treatments. Even after 24 h of LED irradiation, the activity of FAK and ERK appeared in cells treated with red LED light but did not appear in cells treated with green and blue LED lights. Furthermore, the activity of caspase-3 was confirmed along with cell detachment. Therefore, our results suggest that red LED light induced mitogenic effects via low levels of ROS–FAK–ERK, while green and blue LED lights induced cytotoxic effects via cellular stress and apoptosis signaling resulting from high levels of ROS.
Forecasting of SPI and SRI Using Multiplicative ARIMA under Climate Variability in a Mediterranean Region: Wadi Ouahrane Basin, Algeria
Authors: Achite Mohammed , Bazrafshan Ommolbanin , Azhdari Zahra , Wałęga Andrzej , Krakauer Nir , Caloiero Tommaso
Published: March 2022

Water resources have always been a major concern, particularly in arid and semiarid parts of the world. Low precipitation and its uneven distribution in Algeria, along with fast population and agriculture activity increase and, particularly, recent droughts, have made water availability one of the country’s most pressing issues. The objectives of the studies reported in this article are to investigate and forecast the meteorological and hydrological drought in Wadi Ouahrane basin (270 km2) using linear stochastic models known as Autoregressive Integrated Moving Average (ARIMA) and multiplicative Seasonal Autoregressive Integrated Moving Average (SARIMA). In particular, data from 6 precipitation stations and 1 hydrometric station for the period 1972–2018 were used to evaluate the Standardized Precipitation Index (SPI) and the Standardized Runoff Index (SRI) for 12 months. Then, the multiplicative ARIMA model was applied to forecasting drought based on SPI and SRI. As a result, the ARIMA model (1,0,1)(0,0,1)12 for SPI and (1,0,1)(1,0,1)12 for SRI were shown to be the best models for drought forecast. In fact, both models exhibited high quality for SPI and SRI of 0.97 and 0.51 for 1-month and 12-month lead time, respectively, based on validation R2. In general, prediction skill decreases with increase in lead time. The models can be used with reasonable accuracy to forecast droughts with up to 12 months of lead time.
Sleep Loss, Daytime Sleepiness, and Neurobehavioral Performance among Adolescents: A Field Study
Authors: Orna Tzischinsky , Efrat Barel
Published: March 2022

The current study investigates the impact of sleep loss on neurobehavioral functioning and sleepiness in a natural setting among healthy adolescents. Fifty-nine adolescents (32 females) from grades 7 to 12 (mean age of 16.29 ± 1.86 years) participated in the study. All participants wore the actigraph for a continuous five to seven days, including school and nonschool days. Subjective sleepiness and neurobehavioral performance (using the psychomotor vigilance test and the digit symbol substitution test) were measured three times a day on two school days and one nonschool day. The results presented that sleep loss influenced subjective sleepiness reports, showing higher sleepiness scores following sleep loss than following sufficient night sleep. Neurobehavioral functioning across all measurements was also significantly worse following sleep loss. Furthermore, participants performed worse on weekday morning assessments than on assessments at other times of the day following sleep loss. These findings suggest that sleep loss in natural settings has a significant impact on neurobehavioral performance and subjective sleepiness. Our findings have essential implications for public policy on school schedules.
Effect of Texture on Total Energy Consumption of High Frequency Hydraulic Impact Piston Pair
Authors: Mo Hu , Guo Yong , Quan Song
Published: March 2022

Limited by the influence of the traditional clearance seal structure on the leakage and friction loss of piston pair, the energy utilization ratio of the hydraulic impactor is difficult to improve effectively. To solve this problem, a novel micro-texture clearance seal structure of impact piston cylinder was proposed, and an integrated energy consumption evaluation index considering leakage and friction loss of impact piston pair was proposed. Based on the average Reynolds equation, a comprehensive energy consumption analysis model for a textured high-frequency hydraulic impact piston pair was established, and the influence of piston texture parameters on the comprehensive energy consumption under rated working conditions was studied. The results show that the cylindrical texture clearance seal structure provided an effective way to improve the energy utilization ratio of hydraulic impactor, with energy consumption 13~15% less than the traditional structure. Variation of area rates textured made the amplitude value of integrated energy consumption of the piston pair decrease by 4~15%, and the optimum area rate was 0.2~0.4. Depth ratio of texture could also reduce the integrated energy consumption of the piston pair, but the reduction range was small.
Development of Silver Doped Hydroxyapatite Thin Films for Biomedical Applications
Authors: Iconaru Simona Liliana , Predoi Daniela , Ciobanu Carmen Steluta , Motelica-Heino Mikael , Guegan Régis , Bleotu Coralia
Published: March 2022

Silver doped hydroxyapatite [AgHAp, Ca10−xAg(PO4)6(OH)2], due to its antimicrobial properties, is an advantageous material to be used for various coatings. The AgHAp thin films with xAg = 0.05 and xAg = 0.1 were achieved using the spin-coating method. The resulting samples were examined by X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). XRD analysis revealed that the particles of both samples are ellipsoidal. Also, in agreement with the results obtained by XRD measurements, the results of the SEM studies have shown that the particles shape is ellipsoidal. Optical properties of silver doped hydroxyapatite thin films deposited on Si substrate were investigated through Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The results obtained by the two complementary techniques highlighted that the molecular structure of the studied samples is not influenced by the increase of the silver concentration in the samples. Our studies revealed that the surface morphology of the obtained samples consist of uniform and continuous layers. The biocompatibility of the obtained thin films was also evaluated with the aid of human osteosarcoma MG63 (ATCC CRL 1427) cell line. Moreover, the in vitro antifungal activity against Candida albicans fungal strain of the AgHAp thin films was studied and the obtained results revealed their antifungal effect. The results of the biological assays showed that the AgHAp thin films are a very promising material for biomedical applications.
High Entropy Alloys Coatings Deposited by Laser Cladding: A Review of Grain Boundary Wetting Phenomena
Authors: Straumal Boris B. , Klinger Leonid , Kuzmin Alexei , Lopez Gabriel A. , Korneva Anna , Straumal Alexander B. , Vershinin Nikolai , Gornakova Alena S.
Published: March 2022

High-entropy alloys (HEAs) are called also alloys without a main component or multiprincipal alloys. They consist of five, six or more components in more or less equal proportions and possess unique properties. Several dozens of thousands of publications have already been devoted to bulk HEAs, while HEA coatings are just beginning to develop. More than half of the works on the deposition of HEA coatings are devoted to laser cladding. In the laser cladding process, a mixture of powders on a substrate is melted in a focused laser beam, which sequentially scans the substrate. In the heated zone, the powder mixture melts. At the end of the crystallization process, a solidified polycrystal and a small amount of residual melt are found in the heated zone. It is possible that the grain boundaries (GBs) in the solidified polycrystal are incompletely or fully wetted by this liquid phase. In this way, the GB wetting with a melt determines the morphology and microstructure of HEAs coatings. This review analyzes GB wetting in single-phase HEAs, as well as in HEAs containing two or more phases. We analyze how the HEAs’ composition, laser scanning speed, laser beam power, external magnetic field or ultrasonic impact affect the microstructure and GB wetting. It is also shown how the microstructure and GB wetting change over the thickness of the rather thick as well as multilayer coatings deposited using a laser cladding.
Influence of Graphene Oxide Additions on the Corrosion Resistance of a Rust Converter Primer
Authors: Díaz Belén , Nóvoa Xosé Ramón , Pérez Carmen , Rodríguez-Morgado Miguel
Published: March 2022

Graphene oxide (GO) has attractive properties, such as a two-dimensional structure. Because of its hydrophilic characteristic, well-dispersed aqueous solutions are attained. Thus, it is easily incorporated into waterborne resins. For these reasons, in the last years GO nanoparticles have been added to polymers, improving corrosion resistance. This work is focused on the performance of a commercial rust converter (RC) doped with five different RC:GO ratios, namely, 1:0; 1:0.3; 1:0.6; 1:0.9; 1:1.2 (%v/v). The X-ray diffraction technique is used to illustrate the effect of RC and RC + GO additions in the iron oxides. Zeta-potential measurements are performed to assess the surface charge of the GO particles. The corrosion resistances of the rusted samples coated with the five rust converter formulations are studied. The electrochemical impedance spectroscopy (EIS) technique and an electrical equivalent circuit are utilized to explain the experimental results. Additionally, it is found that the optimal RC:GO ratio is between 1:0.3 and 1:0.6. The better corrosion resistance reached is that of the RC:0.3GO ratio.
Evolution of WSe2 Flakes Synthesized by Thermally Assisted Conversion Method
Authors: Marinova Vera , Buchkov Krastyo , Videva Vladimira , Dionisiev Irnik , Minev Nikolay , Strijkova Velichka , Dimov Deyan , Dikov Hristosko , Avramova Ivalina , Rafailov Peter , Dimitrov Dimitre
Published: March 2022

We report the synthesis of tungsten diselenide (WSe2) flakes and continuous layers using an atmospheric pressure thermally assisted conversion (TAC) method, where the tungsten (W) layers were pre-deposited by a magnetron sputtering system onto fused silica substrates. Optical microscopy (OM) and atomic force microscopy (AFM) mapping predominantly revealed the formation of isolated flakes with different shapes, mainly concentrated near the substrate’s edges, which tended to form clusters and to further overlap to continuous layers, moving to the central part of the fused silica substrates. Raman spectroscopy and photoluminescence measurements confirmed the existence of atomically thin flakes and 2H-WSe2 continuous layers. The measured current–voltage characteristics indicated Ohmic behavior under dark conditions and photo illumination. Finally, the demonstrated resistor-like behavior suggested unlimited prospects for WSe2 integration into a variety of heterostructures.
Stability Study of Dielectric Properties of Plasma-Sprayed BaTiO3
Authors: Ctibor Pavel , Straka Libor , Lukáč František , Sedláček Josef
Published: March 2022

Barium titanate coatings were, for the first time, sprayed by a high feed-rate plasma torch with water stabilization of the plasma. Two power levels of the torch were applied for spraying to cover steel substrates. Various substrate preheating levels from 125 °C to 377 °C were used to modify cooling conditions. Microstructure and phase composition including crystallinity quantification were observed. Dielectric measurements proved that the relative permittivity between 300 and 400 coatings is too temperature sensitive over 170 °C but fits the requirements of the EIA temperature coefficient between room temperature and 170 °C. Simultaneously, the loss tangent remains rather low, between 0.02 and 0.07, in a broad range of temperatures and frequencies. Annealing was performed in air to heal the oxygen deficiency, but only modified the microstructure insignificantly. The dielectric properties of as-sprayed and annealed samples were discussed, with the main finding that the temperature coefficient of permittivity was improved by annealing. This study contributes to the search for the suitability of plasma-sprayed BaTiO3 coatings for application in the electrical industry, namely by the optimization of conditions for high feed-rate spraying.
Topological Phases of an Interacting Majorana Benalcazar–Bernevig–Hughes Model
Authors: Maiellaro Alfonso , Illuminati Fabrizio , Citro Roberta
Published: March 2022

We study the effects of Coulomb repulsive interactions on a Majorana Benalcazar–Bernevig–Huges (MBBH) model. The MBBH model belongs to the class of second-order topological superconductors (HOTSC2), featuring robust Majorana corner modes. We consider an interacting strip of four chains of length L and perform a density matrix renormalization group (DMRG) numerical simulation based on a tensor-network approach. Study of the non-local fermionic correlations and the degenerate entanglement spectrum indicates that the topological phases are robust in the presence of interactions, even in the strongly interacting regime.
Developing Eco-Friendly Skin Care Formulations with Microemulsions of Essential Oil
Authors: Lee Sie Huey , Chow Pui Shan , Yagnik Chetan Kantilal
Published: March 2022

With the rising public awareness of environmental issues, consumers are increasingly demanding skin care products that create less environmental impact but still provide the same or even greater efficacy. In the skin care arena, microemulsions have been receiving increased attention as the promising delivery technology of skin care actives. Essential oils such as peppermint oil, lavender oil and eucalyptus oil are purported to have excellent antioxidant and antimicrobial properties that could be used as the eco-friendly alternatives for synthetic antioxidants and preservatives in the skin care formulations. This work therefore seeks to develop eco-friendly skin care formulations based on microemulsions of essential oil. Peppermint oil, lavender oil and eucalyptus oil were used as the oil phase to formulate naringin-loaded microemulsions, which demonstrated similar or better antioxidant and antimicrobial properties compared to the synthetic ones. When formulated into gel form, naringin-loaded microemulsion-gel formulations showed enhanced stability and release profile over their unformulated counterpart. Hence, microemulsions of essential oil developed in this work conferred a 4-fold benefits to the skin care formulations: (1) improved release (membrane permeation) of skin care active, (2) improved stability of skin care active, (3) as an eco-friendly alternative to synthetic antioxidant, and (4) a self-preserving system.
Synthesis, X-ray Single-Crystal Analysis, and Anticancer Activity Evaluation of New Alkylsulfanyl-Pyridazino[4,5-b]indole Compounds as Multitarget Inhibitors of EGFR and Its Downstream PI3K-AKT Pathway
Authors: Salama Eid E. , Althobaiti Ibrahim O. , Haukka Matti , Boraei Ahmed T. A.
Published: March 2022

The alkylation of 3,5-dihydro-4H-pyridazino[4,5-b]indole-4-thione with benzyl bromide, ethyl chloroacetate, and allyl bromide in the presence of potassium carbonate (K2CO3) yielded new alkylsulfanylpyridazino[4,5-b]indole derivatives (i.e., compounds 4–6). Hydrazinolysis of ester 6 resulted in hydrazide 7. The structure of compound 6 was verified by X-ray single-crystal analysis. Among the synthesized compounds, compound 6 exhibited the most promising cytotoxicity toward MCF-7 cells with an IC50 value of 12 µM. It showed potential inhibition activity toward EGFR, PI3K, and AKT in MCF-7 cells, with 0.26-, 0.49-, and 0.31-fold reductions in concentration compared to an untreated control. Additionally, it showed apoptosis-inducing activity in MCF-7 cells (47.98-fold); overall apoptosis increased to 38.87% compared to 0.81% in the untreated control, which disrupted the cell cycle at pre-G1 and S phases. Moreover, compound 6 exhibited good binding affinities toward the tested proteins (EGFR, PI3K, and AKT) and had binding energies ranging from −15.87 to −24.87 Kcal/mol. It also formed good interactions with essential amino acids inside the binding sites. Hence, compound 6 is recommended as an anti-breast cancer chemotherapeutic due to its effects on the EGFR-PI3K-AKT pathway.
Oncologists’ Locus of Control, Compassion Fatigue, Compassion Satisfaction, and the Mediating Role of Helplessness
Authors: Braun Michal , Naor Lee , Hasson-Ohayon Ilanit , Goldzweig Gil
Published: March 2022

The oncology setting may give rise to significant feelings of helplessness among oncologists via patients’ inevitable deaths or suffering. The current study examines whether and how oncologists’ sense of control (locus of control; LOC) influences their compassion fatigue and satisfaction. Methods: Seventy-three oncologists completed the following questionnaires: the Professional Quality of Life scale; Levenson’s Internal, Powerful Others, and Chance scale; the Guilt Inventory, State Guilt subscale; and the Learned Helplessness scale. Results: Oncologists reported high levels of secondary traumatic stress and burnout and moderate levels of compassion satisfaction. A positive association between oncologists’ external LOC and compassion fatigue, and a negative association between oncologists’ internal LOC and compassion fatigue, were found. Helplessness, but not guilt, had a mediating role in these associations. Internal LOC was also positively associated with compassion satisfaction. Conclusions: The current study highlights oncologists as a population at risk of experiencing compassion fatigue and emphasizes oncologists’ locus of control as a predisposition that plays a role in the development of this phenomenon. Additionally, the cognitive as well as the emotional aspects of control were found to be important factors associated with compassion fatigue.
Autophagy Agents in Clinical Trials for Cancer Therapy: A Brief Review
Authors: Mohsen Samiha , Sobash Philip T. , Algwaiz Ghada Fahad , Nasef Noor , Al-Zeidaneen Safaa Abed , Karim Nagla Abdel
Published: March 2022

Autophagy has been of novel interest since it was first demonstrated to have effect in Burkitt’s lymphoma. Since that time, the autophagy agents chloroquine and hydroxychloroquine have become the only FDA (Food and Drug Administration)-approved autophagy inhibitors. While not approved for cancer therapy, there are ongoing clinical trials to evaluate their safety and efficacy. Pevonedistat has emerged as a novel inhibitor through the neddylation pathway and is an autophagy activator. This paper summarizes and presents current clinical trials for hydroxychloroquine (HCQ), chloroquine (CQ), and Pevonedistat for the clinician.
Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer
Authors: García-Sánchez Javier , Mafla-España Mayra Alejandra , Tejedor-Cabrera Carlos , Avellán-Castillo Olga , Torregrosa María Dolores , Cauli Omar
Published: March 2022

Frailty syndrome is associated with poor outcomes, morbidity and premature mortality. We performed a cross-sectional study to evaluate the presence of frailty syndrome based on Fried’s frailty phenotype in post-menopausal women with breast cancer. We further analyzed the association between frailty syndrome with geriatric assessments and the association with the concentration of gonadotropins LH and FSH, estrogens, androgens and the aromatase activity index in the blood. We enrolled 47 post-menopausal women with localized breast cancer (mean age 66.8 ± 1.3 years (range 52–83)) prior to the starting of adjuvant endocrine therapy. Patients were identified as “non-frail” (robust) or “prefrail/frail” if they fulfilled at least one frailty criteria. In order to determine associations among variables and to control for other variables potentially affecting frailty syndrome (age, comorbidity index and previous chemotherapy treatment), we performed a logistic regression analysis. The receiver operating characteristic curve was performed to assess the sensitivity and specificity of the hormonal concentration to discriminate prefrail/frail versus non-frail individuals. Significant positive associations were observed between the severity of frailty syndrome and estrone, FSH and LH concentrations and the aromatase activity index in the blood (p < 0.05). Further research into the role of hormonal biomarkers should be evaluated in follow-up studies in order to recommend their use as suitable biomarkers of frailty syndrome in breast cancer patients.
20 Years of Global Change on the Limnology and Plankton of a Tropical, High-Altitude Lake
Authors: Alcocer Javier , Lugo Alfonso , Fernández Rocío , Vilaclara Gloria , Oliva María Guadalupe , Oseguera Luis A. , Silva-Aguilera Raúl A. , Escolero Óscar
Published: March 2022

The present long-term (1993–2013) study was aimed at identifying the effects of global change on the environmental characteristics and the plankton community of the tropical, high-altitude Lake Alchichica, Puebla, Mexico. We found no statistically significant increasing trend in air temperature, but an increase from 2002 to 2013, and interannual variability in the meteorological variables. Accordingly, the water temperature rose from 1993 to 2003 and remained similar until 2013. Nonetheless, on a longer-term (1966-2018), longer than the period considered in the present study, air and water temperatures showed an increasing trend in the range considered indicative of climate change. The lake displayed a characteristic warm monomictic thermal pattern but exhibited interannual variability. The planktonic community composition and structure remained similar. The small chlorophytes (e.g., Monoraphidium minutum) dominated the phytoplankton abundance, while the large diatom Cyclotella alchichicana dominated the biomass. The calanoid copepod Leptodiaptomus garciai dominated both the zooplankton abundance and biomass. However, as the temperature increased the large-size phytoplankton (i.e., C. alchichicana) abundance and biomass increased, but the small-size phytoplankton abundance and biomass, as well as the adult copepod abundance and biomass, decreased. The increase in temperature could have favoured the large-size over the small-size phytoplankton. In addition, the temperature increase could have negatively affected the calanoid copepods in two different ways—direct association with the negative effects of higher temperatures on the physiology of the copepods, or indirect association with the negative effects of higher temperatures on the small-size phytoplankton, which diminish the copepods’ food resource.
Assessment of Microbial Community Composition Changes in the Presence of Phytoplankton-Derived Exudates in Two Contrasting Areas from Chilean Patagonia
Authors: Valdés-Castro Valentina , González Humberto E. , Giesecke Ricardo , Fernández Camila , Molina Verónica
Published: March 2022

Patagonian fjords and channels in southern Chile are heterogeneous ecosystems characterized by the interaction of estuarine and marine waters influencing physical-chemical conditions and biological assemblages. Besides salinity, microbial communities from estuarine and marine origin are naturally subjected to changing organic matter quality and variable nutrient concentrations. In this study, we tackle the response of the bacterial community from estuarine and marine origins associated with two size classes (<0.7 µm and <1.6 µm) to the addition of sterile phytoplankton-derived exudates (PDE) compared to control conditions (no addition). Picoplanktonic cell abundance, active bacterial composition analyzed through 16S rRNA sequencing, changes in dissolved organic carbon (DOC) and δ13C were determined over 5 and 15 days after PDE addition. Our results showed that the active marine bacteria were richer and more diverse than their estuarine counterparts, and were dominated by Alphaproteobacteria and Gammaproteobacteria, respectively. PDE addition in both the fractions and the sample origin resulted in an enrichment throughout the incubation of Rhodobacteracea and Cryimorphaceae families, whereas Epsilonproteobacteria (Arcobacteraceae) were mainly favored in the estuarine experiments. Picoplankton abundance increased with time, but higher cell numbers were found in PDE treatments in both size classes (>2 × 105 cell mL−1). In all the experiments, DOC concentration decreased after eight days of incubation, but shifts in δ13C organic matter composition were greater in the estuarine experiments. Overall, our results indicate that despite their different origins (estuarine versus marine), microbial communities inhabiting the fjord responded to PDE with a faster effect on marine active bacteria.