bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Response to Almonertinib in a Patient With Metastatic NSCLC Resistant to Osimertinib due to Acquired EGFR L718Q Mutation
Authors: Shen Gang , Shi Lei , Tian Xin , Huang Depei , Chen Hao , Gao Chan , Shen Xudong , Zhang Hushan
Published: December 2021

Osimertinib shows strong clinical activity in first- and second-line treatment of nonsmall-cell lung cancer (NSCLC) patients with epidermal growth factor receptor (EGFR) mutations, especially EGFR T790M. However, when patients develop resistance, there is currently no definite postosimertinib treatment option. Herein, we report a patient with metastatic NSCLC who benefited from almonertinib after developing resistance to osimertinib.
Shugan Hewei Decoction Alleviates Cecum Mucosal Injury and Improves Depressive- and Anxiety-Like Behaviors in Chronic Stress Model Rats by Regulating Cecal Microbiota and Inhibiting NLRP3 Inflammasome
Authors: Yue Yingying , Chen Yu , Liu Hao , Xu Lesi , Zhou Xian , Ming Hao , Chen Xin , Chen Miaoqi , Lin Yunya , Liu Lin , Zhao Yingqian , Liu Songlin
Published: December 2021

Chronic stress is a significant cause of depression, anxiety, and intestinal mucosal injury. Gut microbiota disturbances are also associated with these disorders. Shugan Hewei Decoction (SHD), which is a traditional Chinese medicine formula developed by our team, has shown superior therapeutic effects in the treatment of depression, anxiety, and functional gastrointestinal diseases caused by chronic stress. In this study, we investigated the modulatory effect of SHD on the cecal microbiota and cecum mucosal NOD-like receptor protein 3 (NLRP3) inflammasome in a chronic unpredictable stress (CUS)/social isolation rat model. After the SHD intervention, the CUS model rats showed improvements in their depressive- and anxiety-like behaviors, as well as sustained body weight growth and improved fecal characteristics. SHD improved the cecal microbiota diversity and changed the abundance of six microbial genera. A Spearman’s correlation analysis showed a strong correlation between the NLRP3 inflammasome and CUS-perturbed cecal biomarker microbiota. SHD regulated the excessive expression of NLRP3, ASC, caspase-1, interleukin-1β (IL-1β), and IL-18 in the serum and cecum mucosa induced by CUS, as well as the activation of the Toll-like receptor 4/nuclear factor-κB signaling cascades. Our results reveal the pharmacological mechanisms of SHD and provide a validated therapeutic method for the treatment of depression, anxiety, and cecum mucosal injury.
mTORC1 Signaling Pathway Mediates Chronic Stress-Induced Synapse Loss in the Hippocampus
Authors: Luo Yu-Fei , Ye Xiao-Xia , Fang Ying-Zhao , Li Meng-Die , Xia Zhi-Xuan , Liu Jian-Min , Lin Xiao-Shan , Huang Zhen , Zhu Xiao-Qian , Huang Jun-Jie , Tan Dong-Lin , Zhang Yu-Fei , Liu Hai-Ping , Zhou Jun , Shen Zu-Cheng
Published: December 2021

Background: The mechanistic target of rapamycin complex 1 (mTORC1) signaling has served as a promising target for therapeutic intervention of major depressive disorder (MDD), but the mTORC1 signaling underlying MDD has not been well elucidated. In the present study, we investigated whether mTORC1 signaling pathway mediates synapse loss induced by chronic stress in the hippocampus.

Methods: Chronic restraint stress-induced depression-like behaviors were tested by behavior tests (sucrose preference test, forced swim test and tail suspension test). Synaptic proteins and alternations of phosphorylation levels of mTORC1 signaling-associated molecules were measured using Western blotting. In addition, mRNA changes of immediate early genes (IEGs) and glutamate receptors were measured by RT-PCR. Rapamycin was used to explore the role of mTORC1 signaling in the antidepressant effects of fluoxetine.

Results: After successfully establishing the chronic restraint stress paradigm, we observed that the mRNA levels of some IEGs were significantly changed, indicating the activation of neurons and protein synthesis alterations. Then, there was a significant downregulation of glutamate receptors and postsynaptic density protein 95 at protein and mRNA levels. Additionally, synaptic fractionation assay revealed that chronic stress induced synapse loss in the dorsal and ventral hippocampus. Furthermore, these effects were associated with the mTORC1 signaling pathway-mediated protein synthesis, and subsequently the phosphorylation of associated downstream signaling targets was reduced after chronic stress. Finally, we found that intracerebroventricular infusion of rapamycin simulated depression-like behavior and also blocked the antidepressant effects of fluoxetine.

Conclusion: Overall, our study suggests that mTORC1 signaling pathway plays a critical role in mediating synapse loss induced by chronic stress, and has part in the behavioral effects of antidepressant treatment.
Dysregulation of Intestinal Physiology by Aflatoxicosis in the Gilthead Seabream (Sparus aurata)
Authors: Barany Andre , Oliva Milagrosa , Gregório Silvia Filipa , Martínez-Rodríguez Gonzalo , Mancera Juan Miguel , Fuentes Juan
Published: December 2021

Aflatoxin B1 (AFB1) is a mycotoxin often present in food. This study aimed to understand the physiological effects of AFB1 on the seabream (Sparus aurata) gastrointestinal system. In a first in vitro approach, we investigated ion transport using the short-circuit current (Isc) technique in Ussing chambers in the anterior intestine (AI). Application of apical/luminal AFB1 concentrations of 8 and 16 μM to healthy tissues was without effect on tissue transepithelial electrical resistance (TER), and apparent tissue permeability (Papp) was measured using fluorescein FITC (4 kD). However, it resulted in dose-related effects on Isc. In a second approach, seabream juveniles fed with different AFB1 concentrations (1 and 2 mg AFB1 kg−1 fish feed) for 85 days showed significantly reduced gill Na+/K+-ATPase (NKA) and H+-ATPase (HA) activities in the posterior intestine (PI). Moreover, dietary AFB1 modified Isc in the AI and PI, significantly affecting TER in the AI. To understand this effect on TER, we analyzed the expression of nine claudins and three occludins as markers of intestinal architecture and permeability using qPCR. Around 80% of the genes presented significantly different relative mRNA expression between AI and PI and had concomitant sensitivity to dietary AFB1. Based on the results of our in vitro, in vivo, and molecular approaches, we conclude that the effects of dietary AFB1 in the gastrointestinal system are at the base of the previously reported growth impairment caused by AFB1 in fish.
Bark Transpiration Rates Can Reach Needle Transpiration Rates Under Dry Conditions in a Semi-arid Forest
Authors: Lintunen Anna , Preisler Yakir , Oz Itay , Yakir Dan , Vesala Timo , Hölttä Teemu
Published: December 2021

Drought can cause tree mortality through hydraulic failure and carbon starvation. To prevent excess water loss, plants typically close their stomata before massive embolism formation occurs. However, unregulated water loss through leaf cuticles and bark continues after stomatal closure. Here, we studied the diurnal and seasonal dynamics of bark transpiration and how it is affected by tree water availability. We measured continuously for six months water loss and CO2 efflux from branch segments and needle-bearing shoots in Pinus halepensis growing in a control and an irrigation plot in a semi-arid forest in Israel. Our aim was to find out how much passive bark transpiration is affected by tree water status in comparison with shoot transpiration and bark CO2 emission that involve active plant processes, and what is the role of bark transpiration in total tree water use during dry summer conditions. Maximum daily water loss rate per bark area was 0.03–0.14 mmol m−2 s−1, which was typically ~76% of the shoot transpiration rate (on leaf area basis) but could even surpass the shoot transpiration rate during the highest evaporative demand in the control plot. Irrigation did not affect bark transpiration rate. Bark transpiration was estimated to account for 64–78% of total water loss in drought-stressed trees, but only for 6–11% of the irrigated trees, due to differences in stomatal control between the treatments. Water uptake through bark was observed during most nights, but it was not high enough to replenish the lost water during the day. Unlike bark transpiration, branch CO2 efflux decreased during drought due to decreased metabolic activity. Our results demonstrate that although bark transpiration represents a small fraction of the total water loss through transpiration from foliage in non-stressed trees, it may have a large impact during drought.
Trialogue Meetings: Engaging Citizens and Fostering Communities of Wellbeing Through Collective Dialogue
Authors: Mac Gabhann Liam , Dunne Simon
Published: December 2021

Community-based participatory approaches are widely recognized as valuable methods for improving mental health and well-being by enabling a greater sense of liberty among participants, through the development of equitable policies and practices, which accommodate a range of diverse perspectives. One such approach, “Trialogue Meetings,” has been found to encourage disclosure and dialogue surrounding mental health, facilitate the growth and development of communities in relation to people’s experience of mental health difficulties, service provider and community response. Emerging in the 1990s because of perceived and felt inequitable relations between people with lived experience of mental health difficulties, family members of people with mental health difficulties and professionals providing mental health service provision. This approach has been shown to successfully reduce stigma and discrimination and improve relations between stakeholders in community and mental health care settings. Trialogue Meetings incorporate Open Dialogue methods to allow multiple stakeholder groups to participate in conversations around a given topic and enable the creation of a common language and mutual understanding. Trialogue Meetings have added benefits of allowing individuals to express themselves better, gain a sense of relationality and community with others and address predetermined power hierarchies with prescribed responses to people’s experiences. In this perspective, we present an outline for Trialogue Meetings as a medium for enhancing wellbeing, providing a transformative empowering process for deliberate discursive practice and engaging citizens through sustained collective dialogue.
“Desire Is Like a Dreadful Monster”: Analysis of Extended Metaphors in L2 Argumentative Essays by Chinese Learners of English
Authors: Lu Qiuyun
Published: December 2021

This article explores the use, function, and understanding of extended metaphors in L2 argumentative essays by Chinese learners of English. The analysis starts with the identification of linguistic metaphors and extended metaphors in 72 argumentative texts produced by 37 intermediate Chinese English majors. The function of extended metaphors is then analyzed by adopting the bottom-up approach of establishing systematic metaphors from those identified extended metaphors, to draw learners’ communicative intentions in producing extended metaphors. To understand learners’ thinking processes behind using extended metaphors while writing, four of nine writers were interviewed about the process of writing extended metaphors in their texts in the stimulated recall interviews. It is found that extended metaphors, expressed through similes or direct metaphors at strategic stages in L2 argumentative essays, are often the result of learners’ conscious manipulation of L1 in producing L2 for various communicative purposes, such as the desire for vividness, coherence, comprehensibility, when there is a knowledge gap between L1 and L2, and for evaluative and persuasive power. These communicative functions are consistent with the ideational, interpersonal, and textual functions of language, which also coincide and interact with the rhetorical goals of moves and stages in L2 argumentative essays. Metaphoric thinking, L1 influence, and struggling to express meaning and persuade, cited in learners’ thought reports, are major factors triggering extended metaphors. The findings of this article can contribute to the knowledge of learners’ metaphoric competence in L2, which can, in turn, enrich teachers’ metaphor knowledge and draw teachers’ attention to learners’ creative ways of using metaphors and then raise metaphor awareness in L2 writing, teaching, and learning.
Associations Between High Plasma Methylxanthine Levels, Sleep Disorders and Polygenic Risk Scores of Caffeine Consumption or Sleep Duration in a Swiss Psychiatric Cohort
Authors: Laaboub Nermine , Gholam Mehdi , Sibailly Guibet , Sjaarda Jennifer , Delacrétaz Aurélie , Dubath Céline , Grosu Claire , Piras Marianna , Ansermot Nicolas , Crettol Severine , Vandenberghe Frederik , Grandjean Carole , Gamma Franziska , Bochud Murielle , von Gunten Armin , Plessen Kerstin Jessica , Conus Philippe , Eap Chin B.
Published: December 2021

Objective: We first sought to examine the relationship between plasma levels of methylxanthines (caffeine and its metabolites) and sleep disorders, and secondarily between polygenic risk scores (PRS) of caffeine consumption or sleep duration with methylxanthine plasma levels and/or sleep disorders in a psychiatric cohort.

Methods: Plasma levels of methylxanthines were quantified by ultra-high performance liquid chromatography/tandem mass spectrometry. In inpatients, sleep disorder diagnosis was defined using ICD-10 “F51.0,” sedative drug intake before bedtime, or hospital discharge letters, while a subgroup of sedative drugs was used for outpatients. The PRS of coffee consumption and sleep duration were constructed using publicly available GWAS results from the UKBiobank.

Results: 1,747 observations (1,060 patients) were included (50.3% of observations with sleep disorders). Multivariate analyses adjusted for age, sex, body mass index, setting of care and psychiatric diagnoses showed that patients in the highest decile of plasma levels of methylxanthines had more than double the risk for sleep disorders compared to the lowest decile (OR = 2.13, p = 0.004). PRS of caffeine consumption was associated with plasma levels of caffeine, paraxanthine, theophylline and with their sum (β = 0.1; 0.11; 0.09; and 0.1, pcorrected = 0.01; 0.02; 0.02; and 0.01, respectively) but not with sleep disorders. A trend was found between the PRS of sleep duration and paraxanthine levels (β = 0.13, pcorrected = 0.09).

Discussion: Very high caffeine consumption is associated with sleep disorders in psychiatric in- and outpatients. Future prospective studies should aim to determine the benefit of reducing caffeine consumption in high caffeine-consuming patients suffering from sleep disorders.
The Aged Lower Urinary Tract: Deficits in Neural Control Mechanisms
Authors: Hardy Cara C.
Published: December 2021

Bothersome urinary symptoms plague many older adults and disproportionally affect women. Underreporting of symptoms and general stigma/embarrassment associated with incontinence has negatively impacted the availability of treatments, as research cannot be championed if the severity of the problem is not apparent. Available therapeutics have limited efficacy and are often not recommended in aged patients. Lower urinary tract function has a long and rich history in animal studies; while much of the underlying anatomy has been described, including neural control mechanisms, the impact of aging has only just begun to be addressed. Recent work has provided strong evidence that neural control over micturition is significantly impacted by aging processes. This mini review discusses recent findings regarding how aging impacts the neural control mechanisms of micturition.
Computational Models for Diagnosing and Treating Endometriosis
Authors: Mbuguiro Wangui , Gonzalez Adriana Noemi , Mac Gabhann Feilim
Published: December 2021

Endometriosis is a common but poorly understood disease. Symptoms can begin early in adolescence, with menarche, and can be debilitating. Despite this, people often suffer several years before being correctly diagnosed and adequately treated. Endometriosis involves the inappropriate growth of endometrial-like tissue (including epithelial cells, stromal fibroblasts, vascular cells, and immune cells) outside of the uterus. Computational models can aid in understanding the mechanisms by which immune, hormone, and vascular disruptions manifest in endometriosis and complicate treatment. In this review, we illustrate how three computational modeling approaches (regression, pharmacokinetics/pharmacodynamics, and quantitative systems pharmacology) have been used to improve the diagnosis and treatment of endometriosis. As we explore these approaches and their differing detail of biological mechanisms, we consider how each approach can answer different questions about endometriosis. We summarize the mathematics involved, and we use published examples of each approach to compare how researchers: (1) shape the scope of each model, (2) incorporate experimental and clinical data, and (3) generate clinically useful predictions and insight. Lastly, we discuss the benefits and limitations of each modeling approach and how we can combine these approaches to further understand, diagnose, and treat endometriosis.
Assessing Automatic Approach-Avoidance Behavior in an Immersive Virtual Environment
Authors: Degner Juliane , Steep Lea , Schmidt Susanne , Steinicke Frank
Published: December 2021

The use of virtual reality (VR) promises enormous potential for studying human behavior. While approach and avoidance tendencies have been explored in various areas of basic and applied psychology, such as attitude and emotion research, basic learning psychology, and behavior therapy, they have rarely been studied in VR. One major focus of this research is to understand the psychological mechanisms underlying automatic behavioral tendencies towards and away from positively or negatively evaluated stimuli. We implemented a whole-body movement stimulus-response compatibility task to explore approach-avoidance behavior in an immersive virtual environment. We chose attitudinal stimuli—spiders and butterflies—on which people widely agree in their general evaluations (in that people evaluate spiders negatively and butterflies positively), while there is still substantial inter-individual variance (i. e., the intensity in which people dislike spiders or like butterflies). We implemented two parallel approach-avoidance tasks, one in VR, one desktop-based. Both tasks revealed the expected compatibility effects that were positively intercorrelated. Interestingly, however, the compatibility effect in the VR measure was unrelated to participants’ self-reported fear of spiders and stimulus evaluations. These results raise important implications about the usage of VR to study automatic behavioral tendencies.
Subspace Detours Meet Gromov–Wasserstein
Authors: Bonet Clément , Vayer Titouan , Courty Nicolas , Septier François , Drumetz Lucas
Published: December 2021

In the context of optimal transport (OT) methods, the subspace detour approach was recently proposed by Muzellec and Cuturi. It consists of first finding an optimal plan between the measures projected on a wisely chosen subspace and then completing it in a nearly optimal transport plan on the whole space. The contribution of this paper is to extend this category of methods to the Gromov–Wasserstein problem, which is a particular type of OT distance involving the specific geometry of each distribution. After deriving the associated formalism and properties, we give an experimental illustration on a shape matching problem. We also discuss a specific cost for which we can show connections with the Knothe–Rosenblatt rearrangement.
Multiplex CRISPR/Cas9 Mutagenesis of BrVRN1 Delays Flowering Time in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
Authors: Hong Joon Ki , Suh Eun Jung , Park Sang Ryeol , Park Jihee , Lee Yeon-Hee
Published: December 2021

The VERNALIZATION1 (VRN1) gene is a crucial transcriptional repressor involved in triggering the transition to flowering in response to prolonged cold. To develop Chinese cabbage (Brassica rapa L. ssp. pekinensis) plants with delayed flowering time, we designed a multiplex CRISPR/Cas9 platform that allows the co-expression of four sgRNAs targeting different regions of the endogenous BrVRN1 gene delivered via a single binary vector built using the Golden Gate cloning system. DNA sequencing analysis revealed site-directed mutations at two target sites: gRNA1 and gRNA2. T1 mutant plants with a 1-bp insertion in BrVRN1 exhibited late flowering after the vernalization. Additionally, we identified ‘transgene-free’ BrVRN1 mutant plants without any transgenic elements from the GE1 (gene-editing 1) and GE2 generations. All GE2 mutant plants contained successful edits in two out of three BrVRN1 orthologs and displayed delayed flowering time. In GE2 mutant plants, the floral repressor gene FLC1 was expressed during vernalization; but the floral integrator gene FT was not expressed after vernalization. Taken together, our data indicate that the BrVRN1 genes act as negative regulators of FLC1 expression during vernalization in Chinese cabbage, raising the possibility that the ‘transgene-free’ mutants of BrVRN1 developed in this study may serve as useful genetic resources for crop improvement with respect to flowering time regulation.
Significance of Pyrolytic Temperature, Particle Size, and Application Rate of Biochar in Improving Hydro-Physical Properties of Calcareous Sandy Soil
Authors: Alghamdi Abdulaziz G. , Al-Omran Abdulrasoul , Alkhasha Arafat , Alasmary Zafer , Aly Anwar A.
Published: December 2021

Water management and irrigation conservation in calcareous sandy soil are of significant importance for sustaining agricultural production, especially in arid and semi-arid region that facing scarcity of water resources. The changes in hydro-physical characteristics of calcareous sand soil were investigated after date palm waste-derived biochar application in column trials. Significance of pyrolysis temperature (300 °C, 500 °C, and 700 °C), particle size [<0.5 mm (D0.5), 0.5–1 mm (D1), and 1–2 mm (D2)], and application rate (1%, 2.5%, and 5%) were studied. Variations in infiltration rate, intermittent evaporation, and saturated hydraulic conductivity as a function of aforementioned factors were investigated. After amending the top 10-cm soil layer with different biochar and application rates, the columns were subjected to six wetting and drying cycles by applying 25 cm3 tap water per week over a 6-week period. Overall, biochar application resulted in decreased saturated hydraulic conductivity, while improved cumulative evaporation. Specifically, biochar produced at 300 °C and 500 °C demonstrated 10.2% and 13.3% higher cumulative evaporation, respectively., whereas, biochar produced at 700 °C with 5% application rate resulted in decreased cumulative evaporation. Cumulative evaporation increased by 5.0%, 7.7% and, 7.8% for D0.5, D1 and D2 (mm) on average, respectively, as compared with the untreated soil. Thus, biochar with particle size 0.5–1 mm significantly improved hydro-physical properties when applied at 1%. Generally, using biochar produced at medium temperature and small particle size with appropriate application rates could improve the soil hydro-physical properties.
Early Relay Intercropping of Short-Season Cotton Increases Lint Yield and Earliness by Improving the Yield Components and Boll Distribution under Wheat-Cotton Double Cropping
Authors: Wang Guoping , Feng Lu , Liu Liantao , Zhang Yongjiang , Li Anchang , Wang Zhanbiao , Han Yingchun , Li Yabing , Li Cundong , Dong Hezhong
Published: December 2021

Wheat-cotton double cropping has improved crop productivity and economic benefits per unit land area in many countries, including China. However, relay intercropping of full-season cotton and wheat, the most commonly adopted mode, is labor-intensive and unconducive to mechanization. The direct sowing of short-season cotton after wheat (CAW) has been successful, but cotton yields and economic benefits are greatly reduced. Whether the relay intercropping of short-season cotton before the wheat harvest increases cotton yields remains unclear, as does the earliness and fiber quality relative to those for CAW. Therefore, we directly planted short-season cotton after wheat harvest on 15 June (CAW) as the control and interplanted short-season cotton in wheat on 15 May (S1), 25 May (S2) and 5 June (S3), which were 30, 20 and 10 days prior to wheat harvest, respectively, from 2016 to 2018. The crop growth, yield, yield components, boll distribution, and earliness of the cotton were evaluated. The yields and earliness of short-season cotton under relay intercropping were 26.7–30.6% and 20.4–42.9% higher than those under CAW, respectively. Compared with CAW, relay intercropping treatments increased the boll density, boll weight and lint percentage by 5.6–13.1%, 12.5–24.5% and 5.8–12.7%, respectively. The dry matter accumulation and harvest index under the relay intercropping treatments were also greater than those under CAW, which might be attributed to the greater partitioning of dry matter to the seed cotton than to the boll shells. Among the relay intercropping treatments (S1, S2 and S3), the lint yield did not differ, but S1 and S2 were considerably better than S3 based on earliness and fiber quality. The analysis of the within-plant spatial boll distribution showed that more bolls were formed on the lower to middle fruiting branches and at the first fruiting sites for S1 and S2 than for S3 and CAW. Therefore, the increased earliness and fiber quality induced through early relay intercropping (S1 and S2) could be attributed to an improved spatial boll distribution compared to late relay intercropping (S3) or CAW. Conclusively, compared to late relay intercropping and CAW, early relay intercropping considerably increased the lint yield, fiber quality, and earliness by improving the yield components, boll distribution, and dry matter accumulation and partitioning. The relay intercropping of short-season cotton 20 to 30 days before wheat harvest represents a promising alternative to CAW in wheat-cotton double-cropping systems in the Yellow River Basin of China and other regions with similar conditions.
Stability and Social Sustainability of Farm Household Income in Poland in 2003–2020
Authors: Kata Ryszard , Leszczyńska Małgorzata
Published: December 2021

This article analyses the available income of farmer households in Poland in 2003–2020 in the context of their level, variability, and differentiation. The income situation of farmers was analysed from the intra-sectoral perspective and the inter-sectoral perspective by comparing the average monthly available income per capita of farmer households to the income of employees and total households. The research aimed to assess the stability of farmers’ incomes against the background of comparative groups and to assess their social sustainability. We understand farmers’ income parity relative to other socio-professional groups and a similar level of intra-sectoral differentiation. The source of empirical materials was the cyclical statistics of the Central Statistical Office prepared as part of the Household Budget Surveys, published in the years 2004–2021. It was found that despite a significant increase in the real income of farmers’ households in Poland over the studied years, the income disparity of farmers relative to other socio-occupational groups persisted. However, the income gap of farmers in relation to workers from the non-agricultural sector decreased from 35.1% in 2003 to an average of 15.7% in 2004–2020, which is largely due to the support for agriculture from the CAP funds. Compared to the analysed groups, the income of farmers’ households is characterised by the most significant instability and greater diversification. These results indicate a persistent deficit in the social sustainability of farmers’ household income in Poland.
A Mobile Application to Follow Up the Management of Broiler Flocks
Authors: Martinez Angel Antonio Gonzalez , Nääs Irenilza de Alencar , Abe Jair Minoro , Pereira Danilo Florentino
Published: December 2021

Broiler meat is one of the most consumed meats worldwide. The broiler production system poses several challenges for the producer, including maintaining environmental conditions for rearing. The popularization of mobile devices (smartphones) among people, including those with lower incomes, makes it possible for specialist systems to be developed and used for diverse purposes through Apps (mobile application). The present study proposed the development of a mobile application to help farmers follow up on-farm flock management. We retrieved rearing environment and flock data from commercial broiler farms that complied with broiler-producing standards and followed the breeders’ recommendations. Data were organized and normalized to serve as the basis for the software. We specified a performance index based on the average environment and flock-based data. The language used for the application development was Python compatible with the GNU GPL (General Public License), which has a vast library of ready-made functions. For the graphical interface, we selected Kivy and KivyMD framework. The developed mobile application might help farmers evaluate broiler rearing conditions on-farm during the flock’s growth and grade the flock using a performance index.
Energy Efficient Lighting in Plant Factories: Addressing Utilance
Authors: Balasus Jens , Blank Janis , Babilon Sebastian , Hegemann Tim , Khanh Tran Quoc
Published: December 2021

Vertical farming is considered to play a crucial role in future food supply. Until today, the high amount of electrical energy required for artificial lighting has been problematic in this context. Various possibilities for increasing efficiency through adapted lighting conditions have been and are being investigated. However, comparably little attention is paid to increasing utilance, i.e., the amount of photons that can effectively be used by the plant. In this work, a novel targeted lighting strategy is therefore proposed that allows for a dynamic adaptation of the luminaires’ light distribution to match the effective crop size at each stage of plant growth in a fully-automated manner. It is shown that the resulting utilance can significantly be increased compared to standard full-coverage lighting. Moreover, it is found that the proposed strategy is likely to consume less than half of the electrical energy usually required for the latter. An additional increase in system efficiency can be prognosticated and the potential energy savings are estimated based on assumptions of future LED generations derived from literature.
Polyploidy in Industrial Crops: Applications and Perspectives in Plant Breeding
Authors: Trojak-Goluch Anna , Kawka-Lipińska Magdalena , Wielgusz Katarzyna , Praczyk Marcin
Published: December 2021

Polyploidisation is an important process in the evolution of many plant species. An additional set of chromosomes can be derived from intraspecific genome duplication (autopolyploidy) or hybridising divergent genomes and chromosome doubling (allopolyploidy). Special forms of polyploidy are autoallopolyploidy and segmental allopolyploidy. Polyploidy arises from two basic processes: spontaneously occurring disturbances of meiotic division and induced by antimitotic agents’ disruption of mitosis. The first involves the induction and fusion of unreduced gametes, resulting in the formation of triploids and tetraploids. The second process uses antimitotics that disrupt cellular microtubules and prevent chromosome’s sister chromatids motion during anaphase. Colchicine, oryzalin, and trifluralin are the most commonly used antimitotics for inducing polyploids in plants. The exposure time and concentration of the antimitotics and the species, cultivar, genotype, and tissue type affect the efficiency of genome duplication. Polyploids are distinguished from diploids by increased cell size and vegetative parts of plants and increased content of secondary metabolites. Genome duplication generates several changes at the epigenetic level resulting in altered gene expression. Polyploidisation is used in plant breeding to overcome the non-viability and infertility of interspecific hybrids, obtain seedless polyploid cultivars and increase resistance/tolerance to biotic and abiotic factors.
Identification of Candidate Genes and Genomic Regions Associated with Adult Plant Resistance to Stripe Rust in Spring Wheat
Authors: Mourad Amira M. I. , Abou-Zeid Mohamed A. , Eltaher Shamseldeen , Baenziger P. Stephen , Börner Andreas
Published: December 2021

Wheat stripe rust (caused by Puccinia striiformis f. sp. tritici) is a major disease that damages wheat plants and affects wheat yield all over the world. In recent years, stripe rust became a major problem that affects wheat yield in Egypt. New races appeared and caused breakdowns in the resistant genotypes. To improve resistance in the Egyptian genotypes, new sources of resistance are urgently needed. In the recent research, a set of 95 wheat genotypes collected from 19 countries, including Egypt, were evaluated for their resistance against the Egyptian race(s) of stripe rust under field conditions in the two growing seasons 2018/2019 and 2019/2020. A high genetic variation was found among the tested genotypes. Single marker analysis was conducted using a subset of 71 genotypes and 424 diversity array technology (DArT) markers, well distributed across the genome. Out of the tested markers, 13 stable markers were identified that were significantly associated with resistance in both years (p-value ≤ 0.05). By using the sequence of the DArT markers, the chromosomal position of the significant DArT markers was detected, and nearby gene models were identified. Two markers on chromosomes 5A and 5B were found to be located within gene models functionally annotated with disease resistance in plants. These two markers could be used in marker-assisted selection for stripe rust resistance under Egyptian conditions. Two German genotypes were carrying the targeted allele of all the significant DArT markers associated with stripe rust resistance and could be used to improve resistance under Egyptian conditions.
A Natural Language Interface to Relational Databases Using an Online Analytic Processing Hypercube
Authors: Hazboun Fadi H. , Owda Majdi , Owda Amani Yousef
Published: December 2021

Structured Query Language (SQL) is commonly used in Relational Database Management Systems (RDBMS) and is currently one of the most popular data definition and manipulation languages. Its core functionality is implemented, with only some minor variations, throughout all RDBMS products. It is an effective tool in the process of managing and querying data in relational databases. This paper describes a method to effectively automate the conversion of a data query from a Natural Language Query (NLQ) to Structured Query Language (SQL) with Online Analytical Processing (OLAP) cube data warehouse objects. To obtain or manipulate the data from relational databases, the user must be familiar with SQL and must also write an appropriate and valid SQL statement. However, users who are not familiar with SQL are unable to obtain relevant data through relational databases. To address this, we propose a Natural Language Processing (NLP) model to convert an NLQ into an SQL query. This allows novice users to obtain the required data without having to know any complicated SQL details. The model is also capable of handling complex queries using the OLAP cube technique, which allows data to be pre-calculated and stored in a multi-dimensional and ready-to-use format. A multi-dimensional cube (hypercube) is used to connect with the NLP interface, thereby eliminating long-running data queries and enabling self-service business intelligence. The study demonstrated how the use of hypercube technology helps to increase the system response speed and the ability to process very complex query sentences. The system achieved impressive performance in terms of NLP and the accuracy of generating different query sentences. Using OLAP hypercube technology, the study achieved distinguished results compared to previous studies in terms of the speed of the response of the model to NLQ analysis, the generation of complex SQL statements, and the dynamic display of the results. As a plan for future work, it is recommended to use infinite-dimension (n-D) cubes instead of 4-D cubes to enable ingesting as much data as possible in a single object and to facilitate the execution of query statements that may be too complex in query interfaces running in a data warehouse. The study demonstrated how the use of hypercube technology helps to increase system response speed and process very complex query sentences.
Exploring the Ruminal Microbial Community Associated with Fat Deposition in Lambs
Authors: Zhang Yukun , Zhang Xiaoxue , Li Fadi , Li Chong , Zhang Deyin , Li Xiaolong , Zhao Yuan , Wang Weimin
Published: December 2021

Microbial communities of the sheep rumen have been studied extensively; however, their involvement in the regulation of fat deposition is unknown. Herein, we aimed to identify the correlations among fat deposition-related phenotypes and the effect of microbiota on changes in body fat accumulation. The rumen microbiota of 141 lambs was profiled by 16S ribosomal RNA sequencing, and the volatile fatty acids’ (VFAs’) concentrations were quantified by gas chromatography. Subsequently, the animals were grouped according to body mass index (BMI) to compare the microbiota of the rumen among the sheep with different fat deposition levels. Results further revealed differences in terms of the species abundance, diversity, and microbial composition between sheep with different fat deposition levels. Linear discriminant analysis (LDA) Effect Size (LEfSe) analysis and Random Forest (RF) regression analysis identified changes in 29 ruminal bacteria, which may be the main driver for different fat deposition.
Determining Immunoglobulin Content of Bovine Colostrum and Factors Affecting the Outcome: A Review
Authors: Ahmann Johanna , Steinhoff-Wagner Julia , Büscher Wolfgang
Published: December 2021

The immunoglobulin concentration in bovine colostrum should be measured to ensure feeding with sufficient immunoglobulins (≥50 mg immunoglobulin G mL−1). Adequate feeding prevents diseases, promotes development, and has a positive influence on the adult animal. Indirect and direct measurement methods are available for this purpose. Direct measurement methods cannot be easily used in practice; therefore, farmers use indirect methods such as a colostrometer and a refractometer. Many factors influence the immunoglobulin concentration of colostrum; some of them have already been intensively researched. In particular, lactation and temporal aspects play an essential role. Newer aspects such as dry period, seasonal influences, and genetics are gaining importance, but their impact on immunoglobulin content has not been sufficiently investigated. Developments are still needed, especially in data management. This review analyzes the outcome of different studies on the indirect and direct measurement methods and discusses different factors influencing the immunoglobulin concentration of bovine colostrum.
Rumen Development of Artificially-Reared Lambs Exposed to Three Different Rearing Regimens
Authors: Herath Hitihamy M. G. P. , Pain Sarah J. , Kenyon Paul R. , Blair Hugh T. , Morel Patrick C. H.
Published: December 2021

The objective of this study was to examine the effect of three different rearing regimens on rumen development in lambs reared artificially. Romney ram lambs were randomly allocated to one of three treatments: commercial milk replacer fed to 57 d of age and high fibre concentrate pellets (HFP57); commercial milk replacer, high fibre concentrate pellets, and early weaning from milk replacer at 42 d of age (HFP42); high protein milk replacer from 2–16 d of age followed by commercial milk replacer, low fibre concentrate pellets, and early weaning from milk replacer at 42 d of age (LFP42). Lambs were slaughtered at 57 d of age. Volatile fatty acid content in rumen fluid at slaughter was analysed and rumen tissue samples were collected for histological examination. The rumen n-butyric content was greater (p < 0.05) in both LFP42 and HFP42 treatment lambs compared to HFP57 lambs. The n-valeric content was greater (p < 0.05) in LFP42 lambs compared to both HFP57 and HFP42 treatment lambs. Thickness of the rumen dorsal wall determined by ultrasound scanning at 49 d was greater (p < 0.05) in both HFP42 and LFP42 lambs compared to HFP57 lambs. There was an interaction (p < 0.05) between treatment and site of rumen tissue sampling on papillae width, density, and rumen muscular layer thickness. Collectively, early weaning and the provision of a low fibre pellet leads to improved rumen function and physical development.
Application of Radiation Technology in Removing Endocrine Micropollutants from Waters and Wastewaters—A Review
Authors: Bojanowska-Czajka Anna
Published: December 2021

Advanced Oxidation Processes (AOPs) are increasingly being adopted as a post-treatment after conventional wastewater treatment, mainly due to the efficient removal of biodegradable organic micropollutants. Endocrine disruptors are a specific group of such micropollutants. Many scientific studies demonstrate their extremely harmful effects on living organisms, even at low concentrations in water and wastewater. AOPs based on the generation of reactive species using radiation technologies, these being gamma radiation and electron beam, are still not being used to their full potential. This publication presents the application possibilities of using ionizing radiation for the degradation of selected endocrine micropollutants in water and wastewater.
Effects of Timing Noise on Square-Wave Optoelectronic Oscillators
Authors: Citrin David S.
Published: December 2021

Optoelectronic oscillators produce microwave-modulated optical beams without external modulation. The most commonly studied types produces narrow-band output, i.e., optical output modulated by a sinusoid, in which case phase noise determines key figures of merit that limit device performance. Nonetheless, other types of modulated signals have been exhibited by optoelectronic oscillators, including square waves. In this work we provide a theoretical treatment of the power spectral density of a microwave self-modulated optical periodic, but non-sinusoidal, oscillator in the presence of timing noise (as phase noise is only defined for a single sinusoid) and focus on the case of square waves. We consider the effects of timing noise on the power spectral density and autocorrelation function of the modulation signal.
Systemic Insights on the Integration of UN Sustainable Development Goals within the Norwegian Salmon Value Chain
Authors: Abualtaher Mohd , Rustad Turid , Bar Eirin Skjøndal
Published: December 2021

This study proposes a conceptual framework that aims to gain insight into the integration of the sustainable development goals (SDG) within the Norwegian salmon value chain (NSVC). The proposed framework was developed by applying the systems engineering six-step method and validated through empirical findings from the NSVC. The framework’s application highlighted and analyzed the presence of the SDGs in corporate sustainability reports, academic curriculum, research, and governmental policies. This study uncovered the complexity-reduction elements within the system that drive SDG integration and assure their progress. The SDGs provide a global context for sustainability endeavors in the NSVC. A globally expanded value chain has an organic relationship with global sustainability terms and schemes. The existing practice of corporate sustainability annual reporting was found to be a significant channel for SDG communication. The novelty of this study was that it proposed a mind-map to understand SDG integration within an industrial value chain abstracted into three concepts: commitment, communication, and performance measurability.
FEM Analysis of Piezoelectric Resonator Polarization Process
Authors: Novák Josef
Published: December 2021

The polarization of the piezoelectric resonator depends on the direction of the applied electric field. The direction of the applied electric field is determined by the shape of the resonator and the position of the electrodes. In case of resonators with electrodes incompletely covering their bases, an inhomogeneous electric field is generated, which results in an inhomogeneous polarization of the resonator. The resonator will be polarized in some places either in a direction other than the desired one or not polarized at all. The aim of this work is to analyze the polarization process on resonators with electrodes incompletely covering their bases. The physical description is given by the linear piezoelectric equations, the Gaussian equation for the description of the electric field and by Newton’s law of force. On this basis, a FEM model is developed and used to analyze the polarization process. The results of the calculation of the electric field vector distribution are presented. Finally, the areas are identified in which polarization in the desired direction is achieved in the resonator as well as the ones where no polarization occurs.
An Experimental Implementation of a Resilient Graphic Rendering Cluster
Authors: Skala Tibor , Todorovac Mirsad , Kozlovszky Miklós , Maričević Marko
Published: December 2021

In this paper, we describe the challenge of developing a web front that will give an interactive and relatively immediate result without the overhead of complex grid scheduling, in the sense of the grid’s lack of interactivity and need for certificates that users simply do not own. In particular, the local system of issuing grid certificates is somewhat limited to a narrower community compared to that which we wanted to reach in order to popularize the grid, and our desired level of service availability exceeded the use of the cluster for grid purposes. Therefore, we have developed an interactive, scalable web front and back-end animation rendering frame dispatcher to access our cluster’s rendering power with low latency, low overhead and low performance penalty added to the cost of Persistence of Vision Ray rendering. The system is designed to survive temporary or catastrophic failures such as temporary power loss, load shedding, malfunction of rendering server cluster or client hardware, whether through an automatic or a manual restart, as long as the hardware that keeps the previous work and periodically dumped state of the automata is preserved.
Healthy Knee Kinematic Phenotypes Identification Based on a Clustering Data Analysis
Authors: Mezghani Neila , Soltana Rayan , Ouakrim Youssef , Cagnin Alix , Fuentes Alexandre , Hagemeister Nicola , Vendittoli Pascal-André
Published: December 2021

The purpose of this study is to identify healthy phenotypes in knee kinematics based on clustering data analysis. Our analysis uses the 3D knee kinematics curves, namely, flexion/extension, abduction/adduction, and tibial internal/external rotation, measured via a KneeKG™ system during a gait task. We investigated two data representation approaches that are based on the joint analysis of the three dimensions. The first is a global approach that is considered a concatenation of the kinematic data without any dimensionality reduction. The second is a local approach that is considered a set of 69 biomechanical parameters of interest extracted from the 3D kinematic curves. The data representations are followed by a clustering process, based on the BIRCH (balanced iterative reducing and clustering using hierarchies) discriminant model, to separate 3D knee kinematics into homogeneous groups or clusters. Phenotypes were obtained by averaging those groups. We validated the clusters using inter-cluster correlation and statistical hypothesis tests. The simulation results showed that the global approach is more efficient, and it allows the identification of three descriptive 3D kinematic phenotypes within a healthy knee population.