bluuebunny/crossref_metadata_2025 huggingface dataset:
Benefits of heat-killed Lactobacillus acidophilus on growth performance, nutrient digestibility, antioxidant status, immunity, and cecal microbiota of rabbits
Authors: Xia Miao , LI Chong , Wu Diange , Wu Fengyang , Kong Lingchang , Jia Zifan , Han Wenxiao , Chen Saijuan , Fang Wei , Liu Yajuan , Chen Baojiang
Published: March 2024

Introduction

Heat-killed probiotics, as a type of inactivated beneficial microorganisms, possess an extended shelf life and broader adaptability compared to their live counterparts. This study aimed to investigate the impact of heat-killed Lactobacillus acidophilus (L. acidophilus, LA) - a deactivated probiotic on the growth performance, digestibility, antioxidant status, immunity and cecal microbiota of rabbits.

Methods

Two hundred weaned Hyla rabbits were randomly allocated into five equal groups (CON, L200, L400, L600, and L800). Over a 28-day period, the rabbits were fed basal diets supplemented with 0, 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively.

Results

Results revealed a significant reduction in the feed-to-gain ratio (F/G) in the L600 and L800 groups (p < 0.05). Additionally, the L800 group exhibited significantly higher apparent digestibility of crude fiber (CF) and crude protein (CP) (p < 0.05). Regarding digestive enzyme activities, enhanced trypsin and fibrinase activities were observed in the L600 and L800 groups (p < 0.05). Concerning the regulation of the body’s antioxidant status, the L800 group demonstrated elevated levels of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in both serum and ileal tissue (p < 0.05). In terms of immune capacity modulation, serum tumor necrosis factor-α (TNF-α) levels were significantly lower in the L600 and L800 groups (p < 0.05), while immunoglobulin A (IgA) and immunoglobulin M (IgM) levels were higher (p < 0.05). Additionally, the L800 group exhibited a substantial increase in secretory immunoglobulin A (SIgA) levels in the intestinal mucosa (p < 0.05). In comparison to the CON group, the L800 group exhibited a significant increase in the relative abundance of Phascolarctobacterium and Alistipes in the cecum (p < 0.05). Phascolarctobacterium demonstrated a positive correlation with SIgA (p < 0.05), IgM (p < 0.01), and Glutathione peroxidase (GSH-Px) (p < 0.05), while displaying a negative correlation with TNF-α levels (p < 0.05). Concurrently, Alistipes exhibited positive correlations with IgA (p < 0.05), IgM (p < 0.05), SIgA (p < 0.01), GSH-Px (p < 0.05), SOD (p < 0.05), and T-AOC (p < 0.01), and a negative correlation with TNF-α (p < 0.05).

Discussion

In conclusion, the dietary incorporation of 600 mg/kg and 800 mg/kg of heat-killed LA positively influenced the growth performance, nutrient digestibility, antioxidant status, immune capacity and cecal microbiota of rabbits. This highlights the potential benefits of utilizing heat-killed probiotics in animal nutrition.
Application of the Parabola Method in Nonconvex Optimization
Authors: Kolosnitsyn Anton , Khamisov Oleg , Semenkin Eugene , Nelyub Vladimir
Published: March 2024

We consider the Golden Section and Parabola Methods for solving univariate optimization problems. For multivariate problems, we use these methods as line search procedures in combination with well-known zero-order methods such as the coordinate descent method, the Hooke and Jeeves method, and the Rosenbrock method. A comprehensive numerical comparison of the obtained versions of zero-order methods is given in the present work. The set of test problems includes nonconvex functions with a large number of local and global optimum points. Zero-order methods combined with the Parabola method demonstrate high performance and quite frequently find the global optimum even for large problems (up to 100 variables).
Active Flutter Suppression of a Wing Section in the Subsonic, Sonic and Supersonic Regimes by the H∞ Control Method
Authors: Muñoz Álvaro , García-Fogeda Pablo
Published: March 2024

This paper compares various procedures for determining the optimal control law for a wing section in compressible flow. The flow regime includes subsonic, sonic and supersonic flows. For the evolution of the system in the Laplace plane, the present method makes use of the exact unsteady aerodynamic forces in this plane once the control law is established. This is a great advantage over other results previously published, where the unsteady aerodynamics in the Laplace plane are merely approximations of the curve-fitted values in the frequency domain (imaginary axis). A comparison of different control techniques like pole placement, LQR and H-infinity control demonstrates that the H-infinity controller is the optimal choice, exhibiting an H-infinity norm approximately two orders of magnitude lower than the LQR case. Furthermore, the H-infinity controller demonstrates lower pole values than those of the pole placement and LQR compensator, showing the advantage of the H-infinity controller in terms of economic efficiency.
Effect of Preparation Process on the Physicochemical Properties of Activated Carbon Prepared from Corn Stalks
Authors: Xu Beibei , Chang Min , Fu Chengguo , Han Jiale , Wang Yahui , Feng Yipeng , Zhang Zhiping
Published: March 2024

The preparation of activated carbon (AC) from agricultural and forestry wastes is one of the effective methods for resource utilization. In this study, AC was prepared from corn stalk (CS) by pyrolysis, one-step activation, and two-step activation to determine the optimum preparation method. Based on this, a single-factor design was used to investigate the influence of activating agents (KOH, NaOH, KOH/NaOH), activation temperatures (600, 700, 800 °C), and activation times (60, 90, 120 min) on the physicochemical properties of AC. The physicochemical properties of AC were characterized by Thermal gravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), and Cyclic voltammetry (CV). The results showed that the AC obtained by the one-step activation method (KOH, 800 °C, 120 min) exhibited a rich pore structure and excellent electrochemical properties (Ipa = 159.8 μA, Ipc = −169.5 μA). However, for the two-step activation method, the AC exhibited a poor pore structure and electrochemical properties (Ipa = 130.8 μA, Ipc = −129.9 μA). In addition, one-step activation provides high-quality AC in a shorter activation time than two-step activation.
The Quantitative Inhibition Effects of Meteorological Drought on Sugarcane Growth Using the Decision Support System for Agrotechnology Transfer-CANEGRO Model in Lai-bin, China
Authors: Yang Yunchuan , Wang Weiquan , Zhang Huiya , Liao Liping , Wang Tingyan , Yang Jiazhen , Xie Xinchang , Li Xungui
Published: March 2024

Sugarcane is the most important cash crop for producing sugar and the most promising high-yield bioenergy crop in China. Lai-bin is a major sugarcane growing and karst area in the Guangxi Province of China. However, frequent droughts over the years have caused huge losses on sugarcane production in this region. Therefore, the daily quantitative response mechanisms of sugarcane growth to multiple meteorological drought scenarios were discovered in Lai-bin using the DSSAT−CANEGRO model. The daily Standardized Precipitation Evapotranspiration Index (SPEI) was developed to detect the possible scenarios of meteorological drought events over the sugarcane growth periods. The results indicate that, in general, the inhibitory effect on sugarcane growth is enhanced by the increase in the intensity and duration of meteorological drought, which involved cane yield (CY), stem height (SH), leaf area index (LAI), and evapotranspiration (ET). Additionally, a light drought at the seedling stage and a light, moderate, and severe drought at the maturity stage give a promotion effect on sugarcane growth, but its overall CY increase rate is less than 5%. The stem elongation stage is the most sensitive period of sugarcane growth to all scenarios of meteorological drought, and the CY reduction rates reached 7.12%, 16.48%, 18.80%, and 29.05%, when the plants suffered from light, moderate, severe, and extreme meteorological droughts, respectively. Alternate drought–flood scenarios presented a facilitating or an inhibiting effect on sugarcane growth during different periods, which cannot be ignored. In conclusion, the quantitative function relationships between meteorological drought and sugarcane growth are revealed by this study.
Numerical and Experimental Analysis of the Vacuum Corn Seed Degassing System
Authors: Ipate George , Ilie Filip , Fătu Viorel , Voicu Gheorghe
Published: March 2024

Vacuum degassing of seeds is a basic preliminary stage of the treatment process to improve the viability of seeds of various crops. In this work, the degassing process of corn seeds was experimentally and numerically analyzed by removing air or other gases from around the seeds, specifically from the seed coating, in a rough vacuum chamber. Two complementary variants were employed to understand and optimize this process to improve the quality and germination rate of the seeds. The average germination percentage on the first day was about 98%, and the germination speed of 5.0 days. Several experiments were conducted with well-established durations of 10 min and masses of 5 kg and masses of corn seeds at different temperatures to observe and record the behavior of the system, facilitating the modeling of the degasification process in the vacuum compartment. Modeling the degasification operation in the vacuum chamber allowed for determining the pressure profiles on the vacuum chamber and its lid. Numerical simulations were either conducted using a simulation program developed in the Visual Basic Applications (VBA) language for Microsoft Excel to model the degassing process in the vacuum chamber or with the assistance of specialized software (transient structural analysis and simulation program in the ANSYS Workbench environment). Statistical analysis of the correlation between experimental and estimated pressure values revealed that both the proposed mathematical model and the solution method are well-chosen, with differences expressed through the absolute error (EA) being very small, only 1.425 mbar. Structural dynamic analysis carried through the Finite Element Method (FEM) highlights that the chosen materials for manufacturing the vacuum chamber vessel (316 stainless steel—yield strength 225 MPa and tangent modulus 2091 MPa) or the chamber lid (transparent acrylic plastic—yield strength 62.35 MPa and shear modulus 1445.3 MPa) are durable and capable of withstanding the desired pressure and temperature demands in the seed treatment process. Additionally, through structural dynamic analysis, it was possible to study the deformation of system components, providing a detailed perspective on their structural distribution. Thus, the paper aims to improve the quality and survival/germination rate of corn seeds as an important step to improve corn yield through simulations and analyses (numerical and experimental) of the vacuum corn seed degassing system. The degassing process of the vacuum chamber was simulated with a simulation program developed for Microsoft Excel for Microsoft 365 MSO (Version 2401 Build 16.0.17231.20236) 64-bit in the VBA language and software (transient structural dynamic analysis in the ANSYS environment through FEM). Vacuum degassing of corn seeds involves the removal of air or other gases around the seeds or products, which is crucial in various fields such as the food, pharmaceutical, or space technology industries.
Research on the Flexible Gradual Seed-Cleaning Method of the Brush-Type Single-Seed Soybean Planter
Authors: Li Yuhuan , Zhao Shuo , Yang Fazhan , Liu Peng , Li Baogang , Song Quan , Yan Shibin , Li Xian
Published: March 2024

In response to the poor cleaning effect of the brush-type soybean seeder, a lightweight mechanism of the brush-type soybean seeder was optimized and designed. We proposed a progressive seed-cleaning method. Firstly, mechanical analysis was conducted on the soybean seeding process to prevent excessive clearing of individual soybean seeds initially filled into the orifices by the seeding brush. A formula for expressing the force exerted by the seeding brush on stabilizing individual soybean seeds in the orifice was derived. Secondly, an analysis of the progressive seeding principle was performed, and theoretical calculations were carried out for the progressive seeding curves within the x-y plane and along the direction of the z-axis. The progressive seeding curves were determined. Factors affecting the seeding efficiency were analyzed, including the plate’s speed, the initial angle of the seeding brush, and the initial distance between the seeding brush and the seeding plate. Based on the Box–Behnken central composite design theory, using the seeding tray’s rotational speed, the initial angle of the seeding brush, and the initial distance between the seeding brush and the seeding tray as experimental factors, orthogonal experiments were conducted with the leakage rate and the over-cleaning rate as the indicators for the evaluation, and the working parameters were optimized. Validation experiments showed that with a plate speed of 29 rpm, an initial angle of the seeding brush of 54°, and an initial distance between the seeding brush and the seeding plate of 11 mm, the results were as follows: a skip-seeding rate of 1.23% and an over-seeding rate of 0.66%, indicating a significant improvement in seeding performance. A comparative experiment was conducted with non-progressive cleaning brushes, and the test results showed that the progressive cleaning brush reduced the leakage rate by more than 1% and the over-cleaning rate by more than 0.5%.
Assessment of Molecular Diversity and Population Structure of Pakistani Mulberry Accessions Using Retrotransposon-Based DNA Markers
Authors: Mehmood Asim , Dracatos Peter M. , Arshad Linta , Bibi Shabana , Zaheer Ahmad
Published: March 2024

Both morphological and molecular markers have been extensively used to evaluate genetic diversity; however, molecular markers are considered more reliable and can lead to improved reproductive efficiency. This study utilized inter-primer binding site (iPBS) markers to examine the genetic diversity and population structure of thirty mulberry accessions from the districts of Sahiwal and Faisalabad, Pakistan. These mulberry accessions belonged to three species: Morus nigra (n = 13), Morus alba (n = 12), and Morus rubra (n = 5). The use of nine iPBS primers in this study provided a comprehensive understanding of genetic diversity among the selected mulberry accessions. Nine iPBS primers were used in the study and generated 431 bands with allelic frequencies ranging from 21 to 75 and band sizes from 200 to 1500 base pairs. The primer 2230 showed the highest polymorphic information content (PIC) value of 0.47 and the highest Shannon’s information index (I = 0.53). The Morus nigra accessions had the highest levels of expected heterozygosity (He = 0.30), unbiased expected heterozygosity (µHe = 0.33), and Shannon’s information index (I = 0.45). The molecular variance analysis (AMOVA) revealed a high degree of genetic variation, as estimated by the pairwise PhiPT value of 0.21, which was significant at the p < 0.001 *** level. The neighbor joining tree, principal coordinate analysis, and structure analysis grouped the 30 mulberry accessions into four main clusters. The distinct grouping of accessions SWLS14, SWLS6, FSDS30, and SWLS7 validated their notable genetic distinctiveness. Overall, these findings contribute valuable insights into the genetic landscape of mulberry accessions, which are essential for conservation and breeding strategies.
Absorption and Transport of Phosphorus in Nodulated Soybean Plants and Diagnosis of Phosphorus Status Using Xylem Sap Analysis
Authors: Yamamura Yoshiaki , Higuchi Kyoko , Saito Akihiro , Ohyama Takuji
Published: March 2024

Phosphorus (P) is an essential major element for plants. The absorption and transport of P are important for soybean growth and yield, including nodule growth and N2 fixation. Through an analysis of xylem sap, we investigated how nodulated soybean plants absorb PO4 via the roots and transport it to the shoot. The nodulated soybean plants were treated with 0, 50, and 250 μM PO4 concentrations for 1, 3, 7, and 15 days. The PO4 concentration in the xylem sap significantly decreased after 1 day of P deprivation, and then it gradually decreased for 15 days. The high-concentration (250 μM PO4) treatment increased the PO4 concentrations in the xylem sap at 7- and 15-day timepoints but not at the 1- or 3-day timepoints. The soybean plants were treated with 0, 25, 50, 100, 150, 250, and 500 μM PO4 for 3 days. The PO4 absorption rate increased consistently in conjunction with the increase in the PO4 concentration; however, the PO4 concentrations in the xylem sap increased only from 0 to 50 μM PO4 but were constant under higher P concentrations. The soybean plants accumulated extra PO4 in the roots. The PO4 concentration in the xylem sap immediately reflected the P deficiency conditions; thus, this index may be used as an indicator for the diagnosis of P deficiency.
Effect of Application Rates of N and P Fertilizers on Soil Nematode Community Structure in Mollisols
Authors: Ni Xuerong , Zhu Xiangming , Feng Qingxiu , Zhao Dan , Huang Weiwei , Pan Fengjuan
Published: March 2024

Long-term application of chemical fertilizer poses an environmental threat to belowground ecosystems. However, the impact of nitrogen (N) or phosphorus (P) fertilizers on soil biodiversity and the conditions of soil food web remains largely unknown. Soil nematodes are the most abundant multicellular soil animals and serve as excellent bioindicators of soil. Here, we investigated soil nematode communities and food web structure in a long-term experiment with different application rates of N and P fertilizers in northeast China. The application of N and P fertilizers increased the abundance of bacterivores but suppressed the abundance of omnivores and predators. The abundance of bacterivores exhibited an increasing trend, while that of omnivores and predators showed a decreasing trend with increasing rates of N and P fertilizers. Plant parasites displayed a decreasing trend in response to N fertilizer, but not to P fertilizer. N and P fertilizers also altered nematode functional guild composition, with N fertilizer increasing the abundance of Ba1, and P fertilizer increasing the abundance of Fu2 and Ba3. Nonmetric multidimensional scaling (NMDS) analysis revealed apparent successions of nematode communities from no fertilizer soils to high rates of N or P fertilizer soils at both the genus and functional guild levels. Furthermore, N and P fertilizers resulted in different nematode communities. In terms of nematode food web indices, N fertilizer increased the enrichment index (EI) but reduced the channel index (CI) and structure index (SI), whereas P fertilizer only reduced the SI value. High rates of N and P fertilizers increased the respired carbon of bacterivores but reduced the respired carbon of predators. Mantel tests revealed significant correlations between soil properties and the community composition of both fungivores and omnivores. Among all soil properties, available phosphorus (AP) had the greatest influence on the community structure of soil nematodes. Our findings indicate that N fertilizer has a powerful effect on nematode food web structure, while P fertilizer exerts a stronger effect on soil nematode community composition.
Effects of Harvesting Method on Seed Yield and Seed Quality in Urochloa ruziziensis (cv. ‘OKI-1’ and cv. ‘Br-203’)
Authors: Imura Yoshimi , Nakamura Ichiro , Juntasin Weenaporn , Hossain Mohammad Amzad , Thaikua Sarayut , Poungkaew Rattikan , Kawamoto Yasuhiro
Published: March 2024

Two field trials were conducted in Northern Thailand from 2018 to 2020 to determine the best manual harvesting method for maximizing seed yield and seed quality of two Urochloa ruziziensis cultivars, cv. OKI-1 and cv. Br-203. Four manual seed harvesting methods were implicated in a randomized complete block design with four replicates. The four methods were as follows: knocking seeds from seedheads into a nylon net receptacle and collecting them once every day (T1); knocking seeds from seedheads into a nylon net receptacle and collecting them once every three days (T2); allowing ripe seeds to fall into a nylon net sheet that was stretched as a receptacle beneath the seedheads and collecting seeds once every five days (T3); and covering the tied seedheads with a nylon net bag and collecting seeds once every five days (T4). The highest pure seed yield was obtained from T3 in cv. Br-203 (333.11 kg/ha), as well as the highest trend in cv. OKI-1 (534.67 kg/ha). T3 produced 22–46% and 11–27% more pure germinated seed yields than the other methods in cv. Br-203 and cv. OKI-1, respectively. As a result, T3 was recommended for higher seed yield, convenient seed harvest, and reduced manual seed harvest time.
Differences in Fatty Acid and Central Carbon Metabolite Distribution among Different Tissues of Alfalfa–Rhizobia Symbiotic System
Authors: Lu Bao-Fu , Kang Wen-Juan , Shi Shang-Li , Guan Jian , Jing Fang , Wu Bei
Published: March 2024

Fatty acid and central carbon metabolism are crucial energy metabolism reactions. However, to date, few studies have examined their distribution characteristics within the alfalfa–rhizobia symbiotic system. To clarify the distributional differences and accumulation rates of fatty acids and central carbon with this system, we measured the plant phenotype, nodule formation, nitrogen fixation capacity, and key nitrogen metabolism enzyme activities of Medicago sativa ‘Gannong No. 9’ 35 days post-inoculation (dpi) with Sinorhizobia meliloti LL11. Additionally, we employed targeted metabolomics to analyze central carbon and fatty acid metabolites in various tissue samples of symbiotic and control (C.K.) plants, as well as in S. meliloti LL11. We found that plant height; root length; aboveground fresh and dry weights; underground fresh and dry weights; and nitrate reductase, nitrogen reductase, glutamine synthetase, and glutamate synthase activities were significantly higher in the leaves and roots of symbiotic plants than in those of C.K. plants. Compared to symbiotic plants, C.K. plants exhibited higher total central carbon and fatty acid metabolite content, accounting for 38.61% and 48.17% of C.K. plants, respectively. We detected 32 central carbon and 40 fatty acid metabolites in S. meliloti LL11, with succinate (343,180.8603 ng·mL−1) and hexadecanoic acid (4889.7783 ng·mL−1) being the most. In both symbiotic and C.K. plants, central carbon metabolite was considerably higher than the fatty acid metabolite central. Moreover, the carbon metabolites found in symbiotic plants were primarily distributed in pink nodule roots (PNRs), with malate exhibiting the highest content (4,800,612.3450 ng·g−1), accounting for 53.09% of total central carbon metabolite content. Fatty acid metabolites were mainly found in pink root nodules (P.N.s), which are sites of nitrogen fixation. Trans-10-nonadecenoic acid and hexadecanoic acid exhibited the highest contents, comprising >15% of the total fatty acid metabolite content. We found that petroselaidic acid is only present in P.N., which seems to be closely related to the nitrogen fixation reaction in P.N. In general, symbiotic plants transfer central carbon metabolites to nodules via PNRs to drive nitrogen fixation. However, in P.N.s, these metabolites are limited, leading to accumulation in PNRs. Fatty acid metabolites, crucial for nitrogen fixation, are prevalent in P.N.s. Conversely, C.K. plants without nitrogen fixation distribute these metabolites primarily to the stems, emphasizing growth. This study provides new insights into the energy metabolism of symbiotic nitrogen fixation.
Effects of Salinity Accumulation on Physical, Chemical, and Microbial Properties of Soil under Rural Domestic Sewage Irrigation
Authors: Wang Weihan , Zhang Dandan , Kong Hao , Zhang Gengtao , Shen Feng , Huang Zhiping
Published: March 2024

Under irrigation with saline wastewater, SO42−, Cl−, and Ca2+ aggregated in the topsoil, and Mg2+ was significantly higher in the deeper soil than in the topsoil and 40 cm soil layers. The abundance of Zoopagomycota, Ascomycota, Mortierellomycota, Basidiomycota, Chytridiomycota, Rozellomycota, Blastocladiomycota, Monoblepharomycota, Mucoromycota and Olpidiomycota in the surface soil was influenced by Mg2+, whereas Ca2+ affected the abundance of Zoopagomycota and Chytridiomycota. In the 40 cm soil layer, Mg2+ and Cl− promoted Actinobacteria, Proteobacteria, Nitrospirae, Firmicutes, Entotheonellaeota, Myxococcota, Gemmatimonadota and Methylomirabilota, whereas they inhibited Planctomycetota, Acidobacteria, Chloroflexi, Patescibacteria and Bacteroidota. In the 80 cm soil layer, SO42− and Cl− promoted Rozellomycota, Mortierellomycota, Chytridiomycota, Ascomycota, and Mucoromycota, but had a negative effect on Glomeromycota, Blastocladiomycota, Olpidiomycota and Monoblepharomycota. The increase in salinity significantly reduced the abundance of the Actinomycetes phylum and the Amoebozoa phylum. Both saprophytic and symbiotic fungi decreased with increasing salinity.
Protection and Modeling in the Use of S, Ca, and Mg Alternatives for Long-Term Sustainable Fertilization Systems
Authors: Rusu Mihai , Mihai Mihaela , Tritean Nicolae , Mihai Valentin C. , Moldovan Lavinia , Ceclan Adrian Ovidiu , Russu Florin , Toader Constantin
Published: March 2024

The complexity of NP and NPK fertilizers in stationary and long-term system yields is proven to determine substantial changes in soil fertility, revealing interaction possibilities related to the chemistry and requirements of other nutrients (S, Ca, Mg, and microelements), while sustainable fertilization can exert control over them through rational fertilization practices and complex nutritive management. Revealing the extent of the modifying effects in the application of S, Ca, and Mg correlated to the soil-plant system conditions relates to the hypothesis of the present research in the context of long-term experiments in Romania at the Office of Pedological and Agrochemical Studies Alba (OSPA Alba) and the Turda Agricultural Research and Development Station (SCDA Turda) with 55 years of a fertilizing effect over the 1967–2022 period, but not solely. This study shows that for sustainable fertilization systems, the complementary application of S, Ca, and Mg with NP and NPK has proven to be effective and unitarily constitutes a measure for the sustainable protection and enhancement of soil fertility.
Trade-Offs between Avoidance of Noxious Electric Shock and Avoidance of Bright Light in Shore Crabs Are Consistent with Predictions of Pain
Authors: Barr Stuart , Elwood Robert W.
Published: March 2024

The suggestion that decapod crustaceans might experience pain has been dismissed by some authors who claim decapods only respond to noxious stimuli by nociceptive reflexes. Because reflexes do not require complex neuronal processing, but pain does, demonstrating reflex responses to noxious stimuli would not support the case for pain. Here, we report an experiment in which shore crabs are repeatedly placed in a light area (20 trials), but the animals can avoid the light by moving to a dark shelter. However, some crabs received an electric shock of 6 or 12 volts each time they entered the shelter. Those receiving either level of shock swiftly reduced their use of shelters and remained in the light. However, the magnitude of shelter avoidance was influenced by the brightness of the arena and the intensity of the shock. Shelter use was subsequently reduced to a greater extent if the shock level was high and the light intensity low. That is, crabs traded their avoidance of shock for their avoidance of bright light. Further, these animals showed avoidance learning and demonstrated activities suggesting anxiety, such as contact with the tank wall in the light area and increased latency to enter shelters when making the decision to enter the shelter if they had received shock in earlier trials. These results fulfil three key behavioural criteria for pain and, thus, are consistent with the idea that decapods can experience pain.
Effects of Dietary Inclusion of a Crude Protein Source Exhibiting the Strongest Attractiveness to Red Sea Bream (Pagrus major) on Growth, Feed Availability, and Economic Efficiency
Authors: Baek Seong Il , Cho Sung Hwoan
Published: March 2024

Dietary incorporation of an attractive feed protein source is a practical method of enhancing feed intake and consequently improving the growth of fish. The attractiveness of 18 crude protein sources to juvenile red sea bream (Pagrus major) and the effects of the dietary inclusion of the crude protein source that exhibited the strongest attractiveness on growth, feed availability, and economic efficiency were determined. Jack mackerel meal (JMM) showed the strongest attractiveness to red sea bream among 18 crude protein ingredients. In an 8-week feeding trial, 810 juveniles were randomly distributed into 27 tanks (30 fish/tank). Nine experimental diets were prepared. The control (Con) diet included 60% fish meal (FM). Various levels (1, 3, 5, 10, 20, 40, 60, and 100%) of JMM were included at the expense of FM in the Con diet, and the resulting diets were named the JMM1, JMM3, JMM5, JMM10, JMM20, JMM40, JMM60, and JMM100 diets, respectively. Fish were hand-fed to apparent satiation twice daily for 8 weeks. The weight gain, specific growth rate, and feed consumption of red sea bream that were fed the JMM40, JMM60, and JMM100 diets were significantly (p < 0.0001 for all) higher than those of the fish fed all other diets. However, dietary JMM inclusion had no remarkable impacts on the feed utilization, biological indices, and chemical composition of the whole body of red sea bream. In terms of the economic view of the study, the economic profit index of red sea bream fed the JMM40, JMM60, and JMM100 diets was significantly (p < 0.0001) greater than that of the fish fed all other diets. In conclusion, the strongest attractiveness to red sea bream among 18 crude protein sources was observed in JMM. The inclusion of more than 40% JMM at the expense of FM in the diet of red sea bream is highly recommended for practical feed formulations to induce remarkable improvement in the growth performance of fish and the economic returns for farmers.
Effects of Five Lipid Sources on Growth, Hematological Parameters, Immunity and Muscle Quality in Juvenile Largemouth Bass (Micropterus salmoides)
Authors: Song Rui , Yao Xinfeng , Jing Futao , Yang Wenxue , Wu Jiaojiao , Zhang Hao , Zhang Penghui , Xie Yuanyuan , Pan Xuewen , Zhao Long , Wu Chenglong
Published: March 2024

This study investigated the effects of fish oil (FO), soybean oil (SO), rapeseed oil (RO), peanut oil (PO) and lard oil (LO) on growth, immunity and muscle quality in juvenile largemouth bass. After 8 weeks, the results showed that FO and RO could increase weight gain and serum alkaline phosphatase and apelin values compared with LO (p < 0.05). Except lower crude lipid contents, higher amounts of n-3 polyunsaturated fatty acids (15.83% and 14.64%) were present in the dorsal muscle of the FO and RO groups. Meanwhile, FO and RO could heighten mRNA levels of immune defense molecules (lysozyme, hepcidin, and transforming growth factor β1) compared with PO (p < 0.05). While SO could increase potential inflammatory risk via rising counts of white blood cells, platelets, neutrophils and monocytes, and mRNA levels of interleukins (IL-1β, IL-8, IL-12 and IL-15), FO and RO could improve hardness, chewiness and springiness through increasing amounts of hydroxyproline, collagen and lysyl oxidase, and mRNA levels of collagen 1α2 and prolyl hydroxylase in the fish dorsal muscle. Moreover, FO and RO could improve firmness through increasing glycogen and glycogen synthase 1 levels when compared with LO (p < 0.05). Therefore, these results could provide dietary lipid source references during the feeding process of adult largemouth bass.
Antimicrobial Resistance and Sports: The Scope of the Problem, Implications for Athletes’ Health and Avenues for Collaborative Public Health Action
Authors: Ožegić Ognjen , Bedenić Branka , Sternak Sunčanica Ljubin , Sviben Mario , Talapko Jasminka , Pažur Iva , Škrlec Ivana , Segedi Ivan , Meštrović Tomislav
Published: March 2024

Antimicrobial resistance (AMR) poses a global threat, leading to increased mortality and necessitating urgent action—however, its impact on athletes and the world of sports has hitherto been neglected. Sports environments (including athletic and aquatic) exhibit high levels of microbial contamination, potentially contributing to the spread of resistant microorganisms during physical activities. Moreover, the literature suggests that travel for sports events may lead to changes in athletes’ gut microbiomes and potentially impact their antibiotic resistance profiles, raising questions about the broader implications for individual and public/global health. The prevalence of Staphylococcus aureus (S. aureus) among athletes (particularly those engaged in contact or collision sports) ranges between 22.4% and 68.6%, with MRSA strains being isolated in up to 34.9% of tested individuals. Factors such as training frequency, equipment sharing, delayed post-training showers, and a history of certain medical conditions are linked to higher colonization rates. Moreover, MRSA outbreaks have been documented in sports teams previously, highlighting the importance of implementing preventive measures and hygiene protocols in athletic settings. In light of the growing threat of AMR, there is a critical need for evidence-based treatment guidelines tailored to athletes’ unique physiological demands to ensure responsible antibiotic use and mitigate potential health risks. While various initiatives—such as incorporating AMR awareness into major sporting events—aim to leverage the broad audience of sports to communicate the importance of addressing AMR, proactive measures (including improved AMR surveillance during large sporting events) will be indispensable for enhancing preparedness and safeguarding both athletes’ and the general public’s health. This narrative review thoroughly assesses the existing literature on AMR and antibiotic usage in the context of sports, aiming to illuminate areas where information may be lacking and underscoring the significance of promoting global awareness about AMR through sports.
Supercritical Fluid Extraction of Bioactive Components from Apple Peels and Their Modulation of Complex I Activity in Isolated Mitochondria
Authors: Aresta Antonella , De Vietro Nicoletta , Cotugno Pietro , Pierri Ciro Leonardo , Trisolini Lucia , Zambonin Carlo
Published: March 2024

Supercritical fluid extraction (SFE) was used to extract bioactive compounds from apple (Malus domestica) peel waste from three different Italian cultivars. The bioactive fractions were extracted applying a temperature of 60 °C and a pressure of 250 bar for 15 min with 20% ethanol as co-solvent, at a flow rate of 2 mL/min. The total polyphenol (TP), anthocyanin (TA), ascorbic acid (AA), and antioxidant activity contents (TACs) were measured, while chromatographic analyses were performed to highlight the differences between the extracts. The Stark cultivar had the highest levels of polyphenols, anthocyanins, and ascorbic acid, while the Royal Gala cultivar showed the highest total antioxidant activity. SFE extracts were then tested for their effect on the mitochondrial NADH–ubiquinone oxidoreductase (Complex I) activity on mitochondria isolated from human embryonic kidney cells (HEK239). The Stark extract showed the most positive response in terms of NADH oxidation. The results obtained in this work highlight the potential of apple peel waste as a source of functional phytocompounds and suggest that Stark cultivar extracts may be exploited for pharmacological applications. This study supports the circular bioeconomy by promoting the use of waste products as a valuable resource.
DPPH Measurements and Structure—Activity Relationship Studies on the Antioxidant Capacity of Phenols
Authors: Yamauchi Moeka , Kitamura Yukino , Nagano Haruka , Kawatsu Junya , Gotoh Hiroaki
Published: March 2024

The consumption of foods that are high in antioxidant capacity is believed to contribute to good health. Moreover, the addition of highly antioxidant compounds to foods is believed to prevent food deterioration. Among the known antioxidants in food, phenols have been identified as the primary antioxidants. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay is a simple, inexpensive, and rapid method widely used to evaluate the antioxidant capacity. Although the results of the DPPH assay depend on conditions such as the reaction time and concentration, the experimental conditions have not been standardized. Further, previous research that compared the antioxidant capacity determined through the DPPH assay largely focused on the differences in the specific substructures of approximately several dozen compounds. In this study, we conducted DPPH assays on 169 phenols under the same experimental conditions and summarized the correlation between their structures and activity. This DPPH assay study is the first single-laboratory investigation of the largest number of components in terms of their Trolox equivalent antioxidant capacities. Further, the analysis method was reproduced in an interlaboratory collaborative study, enabling its application in the reproduction and comparison of measurements in other laboratories.
Ginsenosides Rg1 and Rg2 Activate Autophagy and Attenuate Oxidative Stress in Neuroblastoma Cells Overexpressing Aβ(1-42)
Authors: Liu Ziqi , Cecarini Valentina , Cuccioloni Massimiliano , Bonfili Laura , Gong Chunmei , Angeletti Mauro , Eleuteri Anna Maria
Published: March 2024

Alzheimer’s disease is a neurodegeneration with protein deposits, altered proteolysis, and inflammatory and oxidative processes as major hallmarks. Despite the continuous search for potential therapeutic treatments, no cure is available to date. The use of natural molecules as adjuvants in the treatment of Alzheimer’s disease is a very promising strategy. In this regard, ginsenosides from ginseng root show a variety of biological effects. Here, we dissected the role of ginsenosides Rg1 and Rg2 in modulating autophagy and oxidative stress in neuroblastoma cells overexpressing Aβ(1-42). Key hallmarks of these cellular processes were detected through immunomethods and fluorometric assays. Our findings indicate that ginsenosides are able to upregulate autophagy in neuronal cells as demonstrated by increased levels of LC3II and Beclin-1 proteins and decreased amounts of p62. Simultaneously, an activation of lysosomal hydrolases was observed. Furthermore, autophagy activation promoted the clearance of Aβ(1-42). Rg1 and Rg2 also reduced oxidative stress sources and macromolecule oxidation, promoting NRF2 nuclear translocation and the expression of antioxidant enzymes. Our data further clarify the mechanisms of action of Rg1 and Rg2, indicating new insights into their role in the management of disorders like Alzheimer’s disease.
Reactive Oxygen Species Signaling and Oxidative Stress: Transcriptional Regulation and Evolution
Authors: Hong Yuhang , Boiti Alessandra , Vallone Daniela , Foulkes Nicholas S.
Published: March 2024

Since the evolution of the aerobic metabolism, reactive oxygen species (ROS) have represented significant challenges to diverse life forms. In recent decades, increasing knowledge has revealed a dual role for ROS in cell physiology, showing they serve as a major source of cellular damage while also functioning as important signaling molecules in various biological processes. Our understanding of ROS homeostasis and ROS-mediated cellular signaling pathways has presumed that they are ancient and highly conserved mechanisms shared by most organisms. However, emerging evidence highlights the complexity and plasticity of ROS signaling, particularly in animals that have evolved in extreme environments. In this review, we focus on ROS generation, antioxidative systems and the main signaling pathways that are influenced by ROS. In addition, we discuss ROS’s responsive transcription regulation and how it may have been shaped over the course of evolution.
Influence of Active Flow Control of Synthetic Jet at Suction Surface on the Performance of a Subsonic Axial Compressor Rotor
Authors: Wang Guang , Wu Qing , Liao Yingke , Chu Wuli
Published: March 2024

In order to investigate the effect of a synthetic jet arranged at the suction surface on the aerodynamic performance of an axial flow compressor, a high-speed subsonic axial flow compressor rotor was numerically simulated in this paper. The results showed that after being excited by the synthetic jet, the flow margin of the compressor decreased by 1.3%, but the peak efficiency increased by 0.47%. After analyzing the internal flow field, it was found that although the alternating blowing and suction effect of the synthetic jet can reduce the separation loss of the suction surface, the radial velocity of the flow is higher when stimulated and converges towards the blade tip, resulting in more severe blockage at the blade tip than in the prototype compressor, leading to a slight decrease in stability margin. To balance the stability margin of the compressor, a coupled flow control numerical simulation combining passive control of the casing treatment and active control of the suction surface synthetic jet was subsequently carried out. The results showed that the coupled flow control could exert their respective advantages, resulting in an increase of 17.84% in the compressor flow margin and a decrease of only 0.2% in peak efficiency.
Commonsense-Guided Inductive Relation Prediction with Dual Attention Mechanism
Authors: Duan Yuxiao , Tang Jiuyang , Xu Hao , Liu Changsen , Zeng Weixin
Published: March 2024

The inductive relation prediction of knowledge graphs, as an important research topic, aims at predicting the missing relation between unknown entities with many real-world applications. Existing approaches toward this problem mostly use enclosing subgraphs to extract the features of target nodes to make predictions; however, there is a tendency to ignore the neighboring relations outside the enclosing subgraph, thus leading to inaccurate predictions. In addition, they also neglect the rich commonsense information that can help filter out less convincing results. In order to address the above issues, this paper proposes a commonsense-guided inductive relation prediction method with a dual attention mechanism called CNIA. Specifically, in addition to the enclosing subgraph, we added the multi-hop neighboring relations of target nodes, thereby forming a neighbor-enriched subgraph where the initial embeddings are generated. Next, we obtained the subgraph representations with a dual attention (i.e., edge-aware and relation-aware) mechanism, as well as the neighboring relational path embeddings. Then, we concatenated the two embeddings before feeding them into the supervised learning model. A commonsense re-ranking mechanism was introduced to filter the results that conformed to commonsense. Extensive experiments on WN18RR, FB15k-237, and NELL995 showed that CNIA achieves better prediction results when compared to the state-of-the-art models. The results suggested that our proposed model can be considered as an effective and state-of-the-art solution for inductive relation prediction.
Special Issue on Information Security and Cryptography: The Role of Advanced Digital Technology
Authors: Khan Abdullah Ayub , Por Lip Yee
Published: March 2024

Information security has become a potential prospect that ensures information cannot be breached throughout the process of delivery while being exchanged over the Internet [...]
Fusarium foetens AQF6 Isolated from Amentotaxus ynnanensis H.L.Li as a Prolific Source of Antioxidant Compounds
Authors: Vu Thi Hanh Nguyen , Pham Ngoc Son , Quach Ngoc Tung , Le Phuong Chi , Pham Quynh Anh , Ngo Cao Cuong , Nguyen Van The , Anh Do Hoang , Quang Tran Hong , Chu Hoang Ha , Phi Quyet-Tien
Published: March 2024

Amentotaxus yunnanensis H.L.Li is a threatened conifer distributed only in China, Laos, and Vietnam, whose fungal endophytes have not been exploited yet. The aims of this study were to screen and characterize antioxidant metabolites from endophytic fungi obtained from A. yunnanensis. Using the surface sterilization method, sixteen endophytic fungi were isolated from A. yunnanensis and classified into seven genera including Fusarium, Penicillium, Aspergillus, Diaporthe, Neopestalotiopsis, Purpureocillium, and Simplicillium through the analysis of Internal Transcribed Spacer (ITS) gene sequences. Among the ethyl acetate crude extracts, Fusarium foetens AQF6 contained the highest amount of polyphenol (117.76 ± 0.94 mg gallic acid equivalent/g) and flavonoid (169.01 ± 2.09 mg quercetin equivalent/g). Importantly, 400 µg/mL AQF6 extract exhibited 95.75 ± 1.1% and 85.66 ± 1.91% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging activities, respectively. Chemical investigation led to the isolation and identification of a new bisabolane-type sesquiterpene fusafoetriol (FUS) and 14 known compounds, among which six pure compounds showed free-radical scavenging activity against DPPH and hydroxyl radicals. Under H2O2 stress, the yeast model Saccharomyces cerevisiae treated with 4 mM FUS showed 43.31% increase in viability. FUS has the potential to be applied in nutraceutical, pharmaceutical and food technological applications. Thus, this is the first report demonstrating the presence of endophytic fungi inhabiting A. yunnanensis and their potential as a prolific source of antioxidant metabolites.
Intelligent Evaluation of Chinese Hard-Pen Calligraphy Using a Siamese Transformer Network
Authors: Yan Fei , Lan Xueping , Zhang Hua , Li Linjing
Published: March 2024

The essence of Chinese calligraphy inheritance resides in calligraphy education. However, it encounters challenges such as a scarcity of calligraphy instructors, time-consuming and inefficient manual assessment methods, and inconsistent evaluation criteria. In response to these challenges, this paper introduces a deep learning-based automatic calligraphy evaluation model. Initially, hard-pen handwriting samples from 100 volunteers were collected and preprocessed to create a dataset consisting of 4800 samples, along with the corresponding label files for hard-pen calligraphy evaluation. Subsequently, YOLOv5 was utilized for region detection and character recognition on the evaluation samples to obtain the corresponding standard samples. Lastly, a Siamese metric model, with VGG16 as the primary feature extraction submodule, was developed for hard-pen calligraphy evaluation. To improve feature extraction and propagation, a transformer structure was introduced to extract global information from both the evaluated and standard samples, thereby optimizing the evaluation results. Experimental results demonstrate that the proposed model achieves a precision of 0.75, recall of 0.833, and mAP of 0.990 on the hard-pen calligraphy evaluation dataset, effectively realizing automatic calligraphy evaluation. This model presents a novel approach for intelligently assessing hard-pen calligraphy.