bluuebunny/crossref_metadata_2025 huggingface dataset:
Prognostic Implications of Portal Venous Circulating Tumor Cells in Resectable Pancreatic Cancer
Authors: Choi Young Hoon , Hong Tae Ho , Yoon Seung Bae , Lee In Seok , Lee Myung Ah , Choi Ho Joong , Choi Moon Hyung , Jung Eun Sun
Published: May 2022

Circulating tumor cells (CTCs) are a promising prognostic biomarker for cancers. However, the paucity of CTCs in peripheral blood in early-stage cancer is a major challenge. Our study aimed to investigate whether portal venous CTCs can be a biomarker for early recurrence and poor prognosis in pancreatic cancer. Patients who underwent upfront curative surgery for resectable pancreatic cancer were consecutively enrolled in this prospective study. Intraoperatively, 7.5 mL of portal and peripheral blood was collected, and CTC detection and identification were performed using immunofluorescence staining. Peripheral blood CTC sampling was performed in 33 patients, of which portal vein CTC sampling was performed in 28. The median portal venous CTCs (2.5, interquartile ranges (IQR) 1–7.75) were significantly higher than the median peripheral venous CTCs (1, IQR 0–2, p < 0.001). Higher stage and regional lymph node metastasis were related with a larger number of CTCs (≥3) in portal venous blood. Patients with low portal venous CTCs (≤2) showed better overall (p = 0.002) and recurrence-free (p = 0.007) survival than those with high portal venous CTCs (≥3). If validated, portal CTCs can be used as a prognostic biomarker in patients with resectable pancreatic cancer.
Prognosis of Guillain–Barré Syndrome Linked to COVID-19 Vaccination
Authors: Chua Shaun Kai Kiat , Soh Qian Ying , Saffari Seyed Ehsan , Tan Eng-King
Published: May 2022

There have been increasing reports of Guillain–Barré syndrome (GBS), a rare but debilitating neurological disease, occurring post-COVID-19 vaccination. However, the outcomes and relationships between patient demographics and clinical outcomes of post-COVID-19 vaccination GBS remain unclear. To bridge this gap, our study investigates the outcomes and clinical factors associated with poorer GBS outcomes following COVID-19 vaccination. We conducted a review and pooled analysis of detailed data extracted from 57 published cases with the relevant search strategies and criteria. The groups compared included male versus female patients, 1st dose versus 2nd dose and early onset versus late onset of GBS. Multivariate regression analysis was performed to compare the vaccine type, clinical severity and post-treatment outcomes between these groups of patients. Our results highlight for the first time that females were significantly more likely to have severe clinical presentation and poorer outcomes compared to males. Additionally, viral vector vaccines were the predominant vaccine type administered in early-onset post-COVID-19-vaccination GBS and GBS occurring after the 1st vaccination dose. It was also shown that reported cases of post-vaccination GBS generally displayed a positive response to conventional treatment and had favourable post-treatment outcomes. Through this study, we have established important links and provided assuring evidence for treatment response and post-treatment outcomes of GBS occurring post-COVID-19 vaccination. While the COVID-19 vaccination brings about much greater benefits than risks, our findings provide further impetus for greater vigilance in certain patient groups and more studies to explore the mechanisms behind these links.
Uric Acid and Cortisol Levels in Plasma Correlate with Pre-Competition Anxiety in Novice Athletes of Combat Sports
Authors: de Oliveira Luis Fernando Garcia , Souza-Junior Tácito Pessoa , Fechio Juliane Jellmayer , Gomes-Santos José Alberto Fernandes , Sampaio Ricardo Camões , Vardaris Cristina Vasconcelos , Lambertucci Rafael Herling , de Barros Marcelo Paes
Published: May 2022

Pre-competition anxiety is very prevalent in novice athletes, causing stress and drastic decreases in their performances. Cortisol plays a central role in the psychosomatic responses to stress and also in the physiology of strenuous exercise. Growing evidence links uric acid, an endogenous antioxidant, with oxidative stress and anxiety, as observed in many depressive-related disorders. We here compared anxiety inventory scores (BAI and CSAI-2), cortisol and biomarkers of oxidative stress in the plasma of novice combat athletes (white and blue belts) before and after their first official national competition, when levels of stress are presumably high. Although the novice fighters did not reveal high indexes of anxiety on questionnaires, significant correlations were confirmed between cortisol and cognitive anxiety (Pearson’s r = 0.766, p-value = 0.002, and a ‘strong’ Bayesian inference; BF10 = 22.17) and between pre-post changes of plasmatic uric acid and somatic anxiety (r = 0.804, p < 0.001, and ‘very strong’ inference; BF10 = 46.52). To our knowledge, this is the first study to report such strong correlations between uric acid and pre-competition anxiety in novice combat athletes. The cause-consequence association between these indexes cannot be directly inferred here, although the interplay between uric acid and anxiety deserves further investigation.
Risk Factors for Ovarian Cancer: An Umbrella Review of the Literature
Authors: Whelan Eilbhe , Kalliala Ilkka , Semertzidou Anysia , Raglan Olivia , Bowden Sarah , Kechagias Konstantinos , Markozannes Georgios , Cividini Sofia , McNeish Iain , Marchesi Julian , MacIntyre David , Bennett Phillip , Tsilidis Kostas , Kyrgiou Maria
Published: May 2022

Several non-genetic factors have been associated with ovarian cancer incidence or mortality. To evaluate the strength and validity of the evidence we conducted an umbrella review of the literature that included systematic reviews/meta-analyses that evaluated the link between non-genetic risk factors and ovarian cancer incidence and mortality. We searched PubMed, EMBASE, Cochrane Database of Systematic Reviews and performed a manual screening of references. Evidence was graded into strong, highly suggestive, suggestive or weak based on statistical significance of the random effects summary estimate and the largest study in a meta-analysis, the number of cases, between-study heterogeneity, 95% prediction intervals, small study effects, and presence of excess significance bias. We identified 212 meta-analyses, investigating 55 non-genetic risk factors for ovarian cancer. Risk factors were grouped in eight broad categories: anthropometric indices, dietary intake, physical activity, pre-existing medical conditions, past drug history, biochemical markers, past gynaecological history and smoking. Of the 174 meta-analyses of cohort studies assessing 44 factors, six associations were graded with strong evidence. Greater height (RR per 10 cm 1.16, 95% confidence interval (CI) 1.11–1.20), body mass index (BMI) (RR ≥ 30 kg/m2 versus normal 1.27, 95% CI 1.17–1.38) and three exposures of varying preparations and usage related to hormone replacement therapy (HRT) use increased the risk of developing ovarian cancer. Use of oral contraceptive pill reduced the risk (RR 0.74, 95% CI 0.69–0.80). Refining the significance of genuine risk factors for the development of ovarian cancer may potentially increase awareness in women at risk, aid prevention and early detection.
Targeted Therapy for Adrenocortical Carcinoma: A Genomic-Based Search for Available and Emerging Options
Authors: Hescheler Daniel Alexander , Hartmann Milan Janis Michael , Riemann Burkhard , Michel Maximilian , Bruns Christiane Josephine , Alakus Hakan , Chiapponi Costanza
Published: May 2022

In rare diseases such as adrenocortical carcinoma (ACC), in silico analysis can help select promising therapy options. We screened all drugs approved by the FDA and those in current clinical studies to identify drugs that target genomic alterations, also known to be present in patients with ACC. We identified FDA-approved drugs in the My Cancer Genome and National Cancer Institute databases and identified genetic alterations that could predict drug response. In total, 155 FDA-approved drugs and 905 drugs in clinical trials were identified and linked to 375 genes of 89 TCGA patients. The most frequent potentially targetable genetic alterations included TP53 (20%), BRD9 (13%), TERT (13%), CTNNB1 (13%), CDK4 (7%), FLT4 (7%), and MDM2 (7%). We identified TP53-modulating drugs to be possibly effective in 20–26% of patients, followed by the Wnt signaling pathway inhibitors (15%), Telomelysin and INO5401 (13%), FHD-609 (13%), etc. According to our data, 67% of ACC patients exhibited genomic alterations that might be targeted by FDA-approved drugs or drugs being tested in current clinical trials. Although there are not many current therapy options directly targeting reported ACC alterations, this study identifies emerging options that could be tested in clinical trials.
Ibrutinib Does Not Impact CCR7-Mediated Homeostatic Migration in T-Cells from Chronic Lymphocytic Leukemia Patients
Authors: Mateu-Albero Tamara , Marcos-Jimenez Ana , Wissmann Stefanie , Loscertales Javier , Terrón Fernando , Stein Jens V. , Muñoz-Calleja Cecilia , Cuesta-Mateos Carlos
Published: May 2022

Bruton’s tyrosine kinase inhibitor ibrutinib has significantly changed treatment landscape in chronic lymphocytic leukemia (CLL). Growing evidence supports ibrutinib to work beyond the effect on tumor cells by means of, for example, restoring functionality of the T-cell compartment and increasing circulating T-cell numbers. Recent evidence suggests T-cell enhanced expansion, rather than increased egress from secondary lymphoid organs (SLO), as a root cause for ibrutinib-induced lymphocytosis. However, whether the latter physiological change is also a consequence of a forced retention in blood remains undisclosed. Since CCR7 is the main chemokine receptor taking over the homing of T-cells from peripheral compartments to lymph nodes and other SLO, we aimed to investigate the impact of ibrutinib on CCR7 functionality in T-cells. To this end, we documented receptor expression in T-cells from a large cohort of ibrutinib-treated CLL patients, and performed different in vivo and in vitro migration models. Overall, our data confirm that CCR7 expression or receptor-mediated migration in CLL T-cells is not affected by ibrutinib. Furthermore, it does not modulate CCR7-driven homing nor nodal interstitial migration. Together, our results support that ibrutinib-induced CLL T-cell accumulation in the blood stream is not derived from an impairment of CCR7-driven recirculation between the SLO and bloodstream, and therefore T-cell expansion is the most plausible cause.
Loco-Regional Therapies in Oligometastatic Adrenocortical Carcinoma
Authors: Roux Charles , Boileve Alice , Faron Matthieu , Lamartina Livia , Delpla Alexandre , Tselikas Lambros , Durand-Labrunie Jérome , Hescot Segolène , de Baere Thierry , Hadoux Julien , Deschamps Frederic , Baudin Eric
Published: May 2022

Objective: The recommended first-line treatment for low-tumor-burden ACC (stage IVa ACC) not amenable to radical resection is mitotane in association with loco-regional treatments (LRs). The aim of this study was to determine the patient population that would benefit the most from LR. Materials and methods: This retrospective monocentric expert center chart review study was performed from 2008 to 2021 and included stage IVa patients (≤2 tumoral organs) treated with LR (either radiotherapy, surgery, or interventional radiology). The primary endpoint was disease control (DC). Correlations between DC, time to systemic chemotherapy (TTC), overall survival (OS), and tumor characteristics were analyzed using Kaplan–Meier survival analysis and Cox’s proportional hazards regression model for multivariate analysis. Results: Thirty-four women (57%) and 26 men with a median age of 48.1 years (IQR: 38.3–59.8) were included. One hundred and nine LRs were performed, with a median of 2 (IQR: 1–3) per patient. DC was achieved in 40 out of 60 patients (66.7%). Patients with DC had a significantly longer TTC (HR: 0.27, p < 0.001) and OS (HR: 0.22, p < 0.001). Patients with less than or equal to 5 metastases (HR: 6.15 (95% CI: 1.88–20.0), p = 0.002) or a maximum metastasis diameter below 3 cm had higher rates of DC (HR: 3.78 (95% CI: 1.09–13.14), p = 0.035). Conclusion: stage IVa ACC patients with ≤5 metastases or a maximum metastasis diameter below 3 cm had favorable responses to LR. We propose the name oligometastatic ACC for this subgroup of patients.
HLA Allele Frequencies and Association with Severity of COVID-19 Infection in Northern Italian Patients
Authors: Guerini Franca Rosa , Bolognesi Elisabetta , Lax Agata , Bianchi Luca Nicola Cesare , Caronni Antonio , Zanzottera Milena , Agliardi Cristina , Albergoni Maria Paola , Banfi Paolo Innocente , Navarro Jorge , Clerici Mario
Published: May 2022

HLA allelic distribution was analysed in a cohort of 96 Northern Italian subjects (53M/43F) (mean age 59.9 ± 13.3 years) from Lombardy who developed COVID-19 during the first two pandemic waves to investigate possible correlations between HLA molecules and disease severity. An important role of HLA- B and HLA-C loci in modulating the clinical severity of COVID-19 disease was identified. In particular, the HLA-B07 supertype was observed to be associated with a significant risk for severe disease; conversely, the HLA-B27 supertype and C*12:02 allele played a protective role as they were associated with milder disease. These associations were confirmed after applying a multinomial regression analysis to adjust the correlation for age, gender and comorbidities with COVID-19 severity. Though the power of results is limited by the small sample size, data herein contribute to shedding light on the role played by genetic background in COVID-19 infection.
AMPK Modulates the Metabolic Adaptation of C6 Glioma Cells in Glucose-Deprived Conditions without Affecting Glutamate Transport
Authors: Belo do Nascimento Inês , Verfaillie Marie , Ates Gamze , Beckers Pauline , Joris Virginie , Desmet Nathalie , Massie Ann , Hermans Emmanuel
Published: May 2022

Energy homeostasis in the central nervous system largely depends on astrocytes, which provide metabolic support and protection to neurons. Astrocytes also ensure the clearance of extracellular glutamate through high-affinity transporters, which indirectly consume ATP. Considering the role of the AMP-activated protein kinase (AMPK) in the control of cell metabolism, we have examined its implication in the adaptation of astrocyte functions in response to a metabolic stress triggered by glucose deprivation. We genetically modified the astrocyte-like C6 cell line to silence AMPK activity by overexpressing a dominant negative mutant of its catalytic subunit. Upon glucose deprivation, we found that C6 cells maintain stable ATP levels and glutamate uptake capacity, highlighting their resilience during metabolic stress. In the same conditions, cells with silenced AMPK activity showed a reduction in motility, metabolic activity, and ATP levels, indicating that their adaptation to stress is compromised. The rate of ATP production remained, however, unchanged by AMPK silencing, suggesting that AMPK mostly influences energy consumption during stress conditions in these cells. Neither AMPK modulation nor prolonged glucose deprivation impaired glutamate uptake. Together, these results indicate that AMPK contributes to the adaptation of astrocyte metabolism triggered by metabolic stress, but not to the regulation of glutamate transport.
Proximity-Induced Magnetism in a Topological Insulator/Half-Metallic Ferromagnetic Thin Film Heterostructure
Authors: Zhang Min , Liu Qiya , Liu Ligang , Zeng Tixian
Published: May 2022

Topological insulator (TI) Bi2Se3 thin films were prepared on half-metallic ferromagnetic La0.7Sr0.3MnO3 thin film by magnetron sputtering, forming a TI/FM heterostructure. The conductivity of Bi2Se3was modified by La0.7Sr0.3MnO3 at high- and low-temperature regions via different mechanisms, which could be explained by the short-range interactions and long-range interaction between ferromagnetic insulator and Bi2Se3 due to the proximity effect. Magnetic and transport measurements prove that the ferromagnetic phase and extra magnetic moment are induced in Bi2Se3 films. The weak anti-localized (WAL) effect was suppressed in Bi2Se3 films, accounting for the magnetism of La0.7Sr0.3MnO3 layers. This work clarifies the special behavior in Bi2Se3/La0.7Sr0.3MnO3 heterojunctions, which provides an effective way to study the magnetic proximity effect of the ferromagnetic phase in topological insulators.
Review of Chosen Isogeny-Based Cryptographic Schemes
Authors: Drzazga Bartosz , Krzywiecki Łukasz
Published: May 2022

Public-key cryptography provides security for digital systems and communication. Traditional cryptographic solutions are constantly improved, e.g., to suppress brute-force attacks. However, Shor’s algorithm suited for quantum computers can break the bedrock of most currently used systems, i.e., the RSA problem and discrete logarithm problem. Post-quantum cryptography can withstand attacks carried out by quantum computers. Several families of post-quantum systems exist; one of them is isogeny-based cryptography. As a main contribution, in this paper, we provide a survey of chosen, fundamental isogeny-based schemes. The target audience of this review is researchers interested in practical aspects of this field of cryptography; therefore the survey contains exemplary implementations. Our goal was not to develop an efficient implementation, but to provide materials that make it easier to analyze isogeny-based cryptography.
Influence of Polymer Latexes on the Properties of High Performance Cement–Based Materials
Authors: Cheng Daxiang , Li Xiaosheng , Gao Xu , Fan Xiaochun , Zhao Rui , Yang Tingli
Published: May 2022

The application of polymer latex provides an additional approach when optimizing the properties of high–performance cement–based materials. Given that cement–based materials are extraordinarily sensitive to the characteristics and relative content of polymer latexes, identifying their influence is essential for application. This paper investigates the impact of polymer latex types and its relative content on the mechanical properties, hydration product, pore structure, and drying shrinkage of cement–based materials with a low water to binder ratio. The results showed that the mechanical properties, pore structure, and drying shrinkage of the styrene butadiene latex modified samples were better than those of ethylene vinyl acetate latex and silicone acrylic latex modified ones. Incremental increases to the polymer to cement ratio induces a slight reduction on compressive strength, and styrene–butadiene latex promotes tensile strength. Polymer latex hinders the transformation from ettringite to monosulfate, reducing the bound water and portlandite generated. Increasing the polymer content induces more significant stretching in the vibration peak of SO4 from ettringite. Further incorporation of polymer latex contributes to ettringite precipitation and the decline of harmful pores. Polymer addition also results in lower drying shrinkage compared with the reference group.
The Bacterial Composition and Diversity in a Eucalyptus pellita Plantation in South Sumatra, Indonesia
Authors: Lelana Neo Endra , Utami Sri , Widyati Enny , Puspitaningtyas Dwi Murti , Yulianti  , Supriadi Bambang , Oktarina Seva , Priatna Deni
Published: May 2022

Plantation forests have been strongly established in Indonesia, with Acacias and Eucalyptus as the most common species. Using a single species in a large plantation may affect its sustainability because of the threat from biotic and abiotic factors. The soil microbiome is key to an ecological process strongly associated with both biotic and abiotic factors. However, research aiming to understand soil microbial communities in plantation forests in Indonesia is still limited. We analyzed the soil bacterial communities from six sites of plantation forests and three sites of conservation areas representing natural forest ecosystems. We produced approximately 140,136 reads from nine soil samples and generated 2385 total OTUs from the reads. The ten most abundant phyla were Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Firmicutes, Bacteroidetes, Verrucomicrobia, Nitrospirae, Gemmatimonadetes, and Planctomycetes. At the phylum level, the relative abundance of microbes in E. pellita plantation forests and natural forests was similar, except for Bacteroidetes. The richness and diversity of the microbiomes were slightly lower in the plantation forests than in the natural forests. Minor variations in the soil’s chemical properties may be responsible for the variations in the microbiome between natural and plantation forests. According to RDA, the K, total N, and organic C were positively correlated with the bacterial diversity, while the pH was negatively correlated. There was a positive correlation between the abundance of Bacteroidetes and the K content. However, there is not much information regarding this relationship.
Hardwood Grain Image Restoration and Enhancement via Gaussian Histogram Specification and Adaptive Color Adjustment
Authors: Mao Jingjing , Wu Zhihui
Published: May 2022

Hardwood is widely used in the surface decoration of furniture and wood products due to its rich texture and durable surface, and the improvement of wood grain images is vital to promote the aesthetics of wood surfaces. In order to restore the Gaussian distribution of distorted wood grain images and reproduce a sharp and clear wood surface, a Gaussian histogram specification algorithm based on the constant mean and variance values of red (R), green (G), and blue (B), and an adaptive color adjustment algorithm based on the color extension of R, G, and B histograms was proposed, respectively. Objective evaluation methods of histogram distribution, colorfulness index, contrast index, and sharpness index were used independently to evaluate the visual effect of the images processed by the two algorithms. Objective and subjective evaluation results showed that although the Gaussian method had only a small influence on the visual effect of hardwood grain images, it could restore the distorted images by repairing the irregular color points to weaken the adverse impact on visual impression. Meanwhile, extra attention should be paid to the processing of images with prominent uneven color transitions, because the Gaussian method might have an imperceptible smoothing or enhancing effect. The adaptive color adjustment method had a favorable enhancement effect on most hardwood grain images. However, the color extension coefficients of the over-enhanced images should be reduced to eliminate overcompensation and color shift. Compared with the traditional enhancement method unsharp mask (USM) and the methods designed for sand-degraded images and underwater images, the proposed adaptive color adjustment at the 1.5 coefficient could effectively enhance the images from the perspective of wood grain visibility and color retention.
Fraud Detection Using Neural Networks: A Case Study of Income Tax
Authors: Murorunkwere Belle Fille , Tuyishimire Origene , Haughton Dominique , Nzabanita Joseph
Published: May 2022

Detecting tax fraud is a top objective for practically all tax agencies in order to maximize revenues and maintain a high level of compliance. Data mining, machine learning, and other approaches such as traditional random auditing have been used in many studies to deal with tax fraud. The goal of this study is to use Artificial Neural Networks to identify factors of tax fraud in income tax data. The results show that Artificial Neural Networks perform well in identifying tax fraud with an accuracy of 92%, a precision of 85%, a recall score of 99%, and an AUC-ROC of 95%. All businesses, either cross-border or domestic, the period of the business, small businesses, and corporate businesses, are among the factors identified by the model to be more relevant to income tax fraud detection. This study is consistent with the previous closely related work in terms of features related to tax fraud where it covered all tax types together using different machine learning models. To the best of our knowledge, this study is the first to use Artificial Neural Networks to detect income tax fraud in Rwanda by comparing different parameters such as layers, batch size, and epochs and choosing the optimal ones that give better accuracy than others. For this study, a simple model with no hidden layers, softsign activation function performs better. The evidence from this study will help auditors in understanding the factors that contribute to income tax fraud which will reduce the audit time and cost, as well as recover money foregone in income tax fraud.
Genome-Wide Association Study of Potential Meat Quality Trait Loci in Ducks
Authors: Guo Qixin , Huang Lan , Bai Hao , Wang Zhixiu , Bi Yulin , Chen Guohong , Jiang Yong , Chang Guobin
Published: May 2022

With continuously increasing living standards and health requirements of consumers, meat quality is becoming an important consideration while buying meat products. To date, no genome-wide association study (GWAS) for copy number variants (CNVs) and single nucleotide polymorphisms (SNPs) has been conducted to reveal the genetic effects on meat quality in ducks. This study analyzed the phenotypic correlation and heritability of fat, water, collagen, and protein content of duck breast muscle. To identify the candidate variants for meat quality, we performed a GWAS using 273 ducks from an F2 population. The results of the SNP GWAS showed that the BARHL2, COPS7B, and CCDC50 genes were associated with fat content; BLM, WDR76, and EOMES with water content; CAMTA1, FGD5, GRM7, and RAPGEF5 with collagen production; and RIMS2, HNRNPU, and SPTBN1 with protein content. Additionally, 3, 7, 1, and 3 CNVs were associated with fat, water, collagen, and protein content, respectively, in duck breast muscle. The genes identified in this study can serve as markers for meat quality. Furthermore, our findings may help devise effective breeding plans and selection strategies to improve meat quality.
Fish as Model Systems to Study Epigenetic Drivers in Human Self-Domestication and Neurodevelopmental Cognitive Disorders
Authors: Anastasiadi Dafni , Piferrer Francesc , Wellenreuther Maren , Benítez Burraco Antonio
Published: May 2022

Modern humans exhibit phenotypic traits and molecular events shared with other domesticates that are thought to be by-products of selection for reduced aggression. This is the human self-domestication hypothesis. As one of the first types of responses to a novel environment, epigenetic changes may have also facilitated early self-domestication in humans. Here, we argue that fish species, which have been recently domesticated, can provide model systems to study epigenetic drivers in human self-domestication. To test this, we used in silico approaches to compare genes with epigenetic changes in early domesticates of European sea bass with genes exhibiting methylation changes in anatomically modern humans (comparison 1), and neurodevelopmental cognitive disorders considered to exhibit abnormal self-domestication traits, i.e., schizophrenia, Williams syndrome, and autism spectrum disorders (comparison 2). Overlapping genes in comparison 1 were involved in processes like limb morphogenesis and phenotypes like abnormal jaw morphology and hypopigmentation. Overlapping genes in comparison 2 affected paralogue genes involved in processes such as neural crest differentiation and ectoderm differentiation. These findings pave the way for future studies using fish species as models to investigate epigenetic changes as drivers of human self-domestication and as triggers of cognitive disorders.
Factors Associated with Caesarean and Peripartum Complications at Southern Mozambique’s Rural Hospitals: A Cross-Sectional Analytical Study
Authors: Chicumbe Sérgio ,   Group POEM, Martins Maria do Rosário Oliveira
Published: May 2022

Information about factors underlying peripartum complications is needed to inform health programs in Mozambique. This retrospective study covered the period from January 2013 to December 2018 and was performed at three rural-district hospitals in southern Mozambique, aiming at assessing factors associated with caesarean and peripartum complications. Data were extracted by clinical criteria-based audits on randomly select clients’ files. Logistical regression was used to identify factors associated with peripartum complications. Amongst 5068 audited files, women mean age was 25 years (Standard Deviation (SD) = 7), gestational age was 38 weeks (SD = 2), 25% had “high obstetric-risk” and 19% delivered by caesarean. Factors significantly associated with caesarean included being transferred [Adjusted Odds Ratio (aOR) =1.8; 95% Confidence Interval (95%CI) = 1.3–2.6], preeclampsia [aOR (95%CI) = 2.0 (1.2–3.3)], age [aOR (95%CI) = 0.96 (0.93–0.99)] and “high obstetric-risk” [aOR (95%CI) = 0.54 (0.37–0.78)]. Factors significantly associated with neonatal complication included mother being transferred [aOR (95%CI) = 2.1 (1.8–2.6)], “high obstetric-risk” [aOR (95%CI) = 1.6 (1.3–1.96)], preeclampsia [aOR (95%CI) = 1.5 (1.2–1.8), mother’s age [aOR (95%CI) = −2% (−3%, −0.1%)] and gestational age [aOR (95%CI) = −8% (−13%, −6%)] increment. This study identified amendable factors associated with peripartum complications in rural referral health settings. Strengthening hospitals’ performance assurance is critical to address the identified factors and improve peripartum outcomes for mothers-neonate dyads.
COVID-19 Pandemic-Revealed Consistencies and Inconsistencies in Healthcare: A Medical and Organizational View
Authors: Araja Diana , Berkis Uldis , Murovska Modra
Published: May 2022

The circumstances of the Coronavirus disease caused by the SARS-CoV-2 virus (COVID-19) pandemic have had a significant impact on global and national developments, affecting the existence of society in all its expressions, as well as the lives of people themselves. In the context of the pandemic, increased attention has been focused on acute measures, but the ending of the pandemic is expected as a resolution of the related healthcare problems. However, there are several indicators that the COVID-19 pandemic might induce long-term consequences for individual and public health. Some of the consequences are inferred and predictable, but there are also areas of medicine that have been indirectly affected by the pandemic, and these consequences have not yet been sufficiently explored. This study is focused on drawing attention to some of the COVID-19 pandemic consistencies and the pandemic-revealed inconsistencies in healthcare. Content analysis and statistical analysis were applied to achieve the aim of the study. The main findings of the study address chronic disease burden (particularly, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)), healthcare governance and organizational issues, and the synergy between health policy perspectives and innovative solutions in practice. The study provides insight into the particular healthcare issues affected by the COVID-19 pandemic, such as the increase in mortality in some diagnoses besides COVID-19 and the possible emergence of a new type of resistance—vaccine-resistance—contemporaneously supporting the identification of the tendencies and currently unnoticed indirect consistencies and inconsistencies revealed by the pandemic.
Correlation of Carotenoids Content and ASTA Values of Pepper (Capsicum chinense) Genetic Resources
Authors: Ko Ho-Cheol , Haile Mesfin , Lee Sukyeung , Hwang Aejin , Lee Gi-An , Choi Yu-Mi , Hahn Bum-Soo , Ro Nayoung
Published: May 2022

In this study, 226 Capsicum chinense genetic resources were analyzed using high-performance liquid chromatography and a spectrophotometer to measure and compare their carotenoid content and American Spice Trade Association (ASTA) color values, respectively. The total carotenoid content and ASTA values of the 226 pepper genetic resources were found to be in the range of 7.04–2430.85 mg/100 g dry weight (DW) and 0–221.32, respectively. Capsanthin, capsorubin, lutein, zeaxanthin, antheraxanthin, violaxanthin, α-carotene, β-carotene, and β-cryptoxanthin were the individual carotenoids studied. The average content of each carotenoid (n = 226) in mg/100 g DW was as follows: capsanthin (325.77), antheraxanthin (136.34), capsorubin (82.36), β-carotene (59.11), violaxanthin (46.54), zeaxanthin (43.21), α-carotene (13.91), β-cryptoxanthin (9.67), and lutein (2.72). A strong positive correlation was observed between total carotenoid content and ASTA value (r = 0.965). Likewise, the individual carotenoid content and ASTA value revealed a strong correlation, such as antheraxanthin (r = 0.964), capsanthin (r = 0.946), and capsorubin (r = 0.858). Three genetic resources with a total carotenoid content above 2000 mg/100 g DW were obtained, such as IT261426 (2430.85 mg/100 g DW), IT183657 (2077.55 mg/100 g DW), and IT261213 (2062.54 mg/100 g DW). The findings of this study will assist in the selection of genetic resources with high carotenoid content and ASTA value that can be used to develop and breed new pepper varieties. Also, detailed results of ASTA value correlation with carotenoids in C. chinense genetic resources are provided.
The Lysine Demethylases KdmA and KdmB Differently Regulate Asexual Development, Stress Response, and Virulence in Aspergillus fumigatus
Authors: Choi Yong-Ho , Lee Min-Woo , Shin Kwang-Soo
Published: May 2022

Histone demethylases govern diverse cellular processes, including growth, development, and secondary metabolism. In the present study, we investigated the functions of two lysine demethylases, KdmA and KdmB, in the opportunistic human pathogenic fungus Aspergillus fumigatus. Experiments with mutants harboring deletions of genes encoding KdmA (ΔkdmA) and KdmB (ΔkdmB) showed that KdmA is necessary for normal growth and proper conidiation, whereas KdmB negatively regulates vegetative growth and conidiation. In both mutant strains, tolerance to H2O2 was significantly decreased, and the activities of both conidia-specific catalase (CatA) and mycelia-specific catalase (Cat1) were decreased. Both mutants had significantly increased sensitivity to the guanine nucleotide synthesis inhibitor 6-azauracil (6AU). The ΔkdmA mutant produced more gliotoxin (GT), but the virulence was not changed significantly in immunocompromised mice. In contrast, the production of GT and virulence were markedly reduced by the loss of kdmB. Comparative transcriptomic analyses revealed that the expression levels of developmental process-related genes and antioxidant activity-related genes were downregulated in both mutants. Taken together, we concluded that KdmA and KdmB have opposite roles in vegetative growth, asexual sporulation, and GT production. However, the two proteins were equally important for the development of resistance to 6AU.
The Great Game Will Never End: Why the Global Financial Crisis Is Bound to Be Repeated
Authors: Blake David
Published: May 2022

This article re-examines key explanations of the Global Financial Crisis—product complexity, behavioural biases in decision making, systemic risk, and regulatory arbitrage and capture—and finds a common underlying cause, namely gaming by personnel at all levels in the banking sector and its regulators. This has enabled banks to use highly leveraged, maturity-mismatched investment strategies, which were designed so that the banks retained the upside rewards, but transferred the downside risks to taxpayers, leading to the privatization of profits and the socialization of losses—behaviour that has been described as ‘banksterism’. Although governments have introduced some significant mitigatory measures, they will not be effective in preventing future financial crises, because they do not and, indeed, cannot provide the appropriate incentives to end the Great Game between bankers and taxpayers, which would involve making bankers, rather than taxpayers, personally liable for losses.
Experimental Determination of Hydrogen Isotope Transport Parameters in Vanadium
Authors: Malo Marta , Peñalva Igor , Azkurreta Jon , Garcinuño Belit , Liu Hao-Dong , Rapisarda David , Zhou Hai-Shan , Luo Guang-Nan
Published: May 2022

Deuterium permeation through vanadium membranes in a wide range of pressures and the temperature range ~250–550 °C was experimentally investigated. Measurements on the same material were carried out in three laboratories with different features for an extended characterization and for cross-check validation. A unified equation for deuterium permeability in pure vanadium (99%) was provided as Φ=1.27×10−4·e−8667/T mol m−1 s−1 Pa−0.5, which represents a significant progress for the characterization of the transport properties in this material, given the spread of data, which can currently be found in the literature. Adsorption and recombination rate constants were also measured for hydrogen and deuterium at low pressure for the same range of temperatures. Finally, the influence of the surface roughness was examined by measuring samples with different surface finish.
Modification of Polyacrylonitrile Ultrafiltration Membranes to Enhance the Adsorption of Cations and Anions
Authors: Kishore Chand Anthony Arvind , Bajer Barbara , Schneider Erik S. , Mantel Tomi , Ernst Mathias , Filiz Volkan , Glass Sarah
Published: May 2022

Ion adsorbing ultrafiltration membranes provide an interesting possibility to remove toxic ions from water. Furthermore, it is also possible to recover valuable elements. In this work, we demonstrate two easy strategies to modify polyacrylonitrile membranes with anion and cation adsorbing groups. The membranes were modified to have positively charged amine groups or negatively charged carboxyl groups. The success of the reactions was confirmed using IR spectroscopy and zeta-potential measurements. The membranes carrying negatively charged groups provided a negative zeta-potential and had an isoelectric point at pH 3.6, while the membranes carrying positively charged groups had a positive zeta-potential in the analyzed pH range. Since only the surface of the polymer was modified, the pore size and permeance of the membranes were not drastically affected. The membranes prepared by both modification strategies had a pure water permeance higher than 1000 L/(m2 h bar) and a water contact angle of 44.3 and 57.2°, respectively. Therefore, the membranes can be operated at low pressures with reasonable flux. Additionally, SEM images showed that the membranes were still open-pored. Adsorption tests using a positively and a negatively charged dye as well as a toxic cation and an anion were performed to analyze the adsorption behavior. Both membranes were able to adsorb the oppositely charged dyes as well as the copper and chromate ions. Therefore, these membranes are good candidates to purify water streams containing hazardous ions.
Shotgun Lipidomics for Differential Diagnosis of HPV-Associated Cervix Transformation
Authors: Starodubtseva Natalia L. , Chagovets Vitaliy V. , Nekrasova Maria E. , Nazarova Niso M. , Tokareva Alisa O. , Bourmenskaya Olga V. , Attoeva Djamilja I. , Kukaev Eugenii N. , Trofimov Dmitriy Y. , Frankevich Vladimir E. , Sukhikh Gennady T.
Published: May 2022

A dramatic increase in cervical diseases associated with human papillomaviruses (HPV) in women of reproductive age has been observed over the past decades. An accurate differential diagnosis of the severity of cervical intraepithelial neoplasia and the choice of the optimal treatment requires the search for effective biomarkers with high diagnostic and prognostic value. The objective of this study was to introduce a method for rapid shotgun lipidomics to differentiate stages of HPV-associated cervix epithelium transformation. Tissue samples from 110 HPV-positive women with cervicitis (n = 30), low-grade squamous intraepithelial lesions (LSIL) (n = 30), high-grade squamous intraepithelial lesions (HSIL) (n = 30), and cervical cancers (n = 20) were obtained. The cervical epithelial tissue lipidome at different stages of cervix neoplastic transformation was studied by a shotgun label-free approach. It is based on electrospray ionization mass spectrometry (ESI-MS) data of a tissue extract. Lipidomic data were processed by the orthogonal projections to latent structures discriminant analysis (OPLS-DA) to build statistical models, differentiating stages of cervix transformation. Significant differences in the lipid profile between the lesion and surrounding tissues were revealed in chronic cervicitis, LSIL, HSIL, and cervical cancer. The lipids specific for HPV-induced cervical transformation mainly belong to glycerophospholipids: phosphatidylcholines, and phosphatidylethanolamines. The developed diagnostic OPLS-DA models were based on 23 marker lipids. More than 90% of these marker lipids positively correlated with the degree of cervix transformation. The algorithm was developed for the management of patients with HPV-associated diseases of the cervix, based on the panel of 23 lipids as a result. ESI-MS analysis of a lipid extract by direct injection through a loop, takes about 25 min (including preparation of the lipid extract), which is significantly less than the time required for the HPV test (several hours for hybrid capture and about an hour for PCR). This makes lipid mass spectrometric analysis a promising method for express diagnostics of HPV-associated neoplastic diseases of the cervix.
On-Chip Integrated Yb3+-Doped Waveguide Amplifiers on Thin Film Lithium Niobate
Authors: Zhang Zhihao , Fang Zhiwei , Zhou Junxia , Liang Youting , Zhou Yuan , Wang Zhe , Liu Jian , Huang Ting , Bao Rui , Yu Jianping , Zhang Haisu , Wang Min , Cheng Ya
Published: May 2022

We report the fabrication and optical characterization of Yb3+-doped waveguide amplifiers (YDWA) on the thin film lithium niobate fabricated by photolithography assisted chemo-mechanical etching. The fabricated Yb3+-doped lithium niobate waveguides demonstrates low propagation loss of 0.13 dB/cm at 1030 nm and 0.1 dB/cm at 1060 nm. The internal net gain of 5 dB at 1030 nm and 8 dB at 1060 nm are measured on a 4.0 cm long waveguide pumped by 976 nm laser diodes, indicating the gain per unit length of 1.25 dB/cm at 1030 nm and 2 dB/cm at 1060 nm, respectively. The integrated Yb3+-doped lithium niobate waveguide amplifiers will benefit the development of a powerful gain platform and are expected to contribute to the high-density integration of thin film lithium niobate based photonic chip.
A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device
Authors: Yu Chengzhuang , Li Shanshan , Wei Chunyang , Dai Shijie , Liang Xinyi , Li Junwei
Published: May 2022

A fluorescence microscope is one of the most important tools for biomedical research and laboratory diagnosis. However, its high cost and bulky size hinder the application of laboratory microscopes in space-limited and low-resource applications. Here, in this work, we proposed a portable and cost-effective fluorescence microscope. Assembled from a set of 3D print components and a webcam, it consists of a three-degree-of-freedom sliding platform and a microscopic imaging system. The microscope is capable of bright-field and fluorescence imaging with micron-level resolution. The resolution and field of view of the microscope were evaluated. Compared with a laboratory-grade inverted fluorescence microscope, the portable microscope shows satisfactory performance, both in the bright-field and fluorescence mode. From the configurations of local resources, the microscope costs around USD 100 to assemble. To demonstrate the capability of the portable fluorescence microscope, we proposed a quantitative polymerase chain reaction experiment for meat product authenticating applications. The portable and low-cost microscope platform demonstrates the benefits in space-constrained environments and shows high potential in telemedicine, point-of-care testing, and more.
Markov Transition Field Enhanced Deep Domain Adaptation Network for Milling Tool Condition Monitoring
Authors: Sun Wei , Zhou Jie , Sun Bintao , Zhou Yuqing , Jiang Yongying
Published: May 2022

Tool condition monitoring (TCM) is of great importance for improving the manufacturing efficiency and surface quality of workpieces. Data-driven machine learning methods are widely used in TCM and have achieved many good results. However, in actual industrial scenes, labeled data are not available in time in the target domain that significantly affect the performance of data-driven methods. To overcome this problem, a new TCM method combining the Markov transition field (MTF) and the deep domain adaptation network (DDAN) is proposed. A few vibration signals collected in the TCM experiments were represented in 2D images through MTF to enrich the features of the raw signals. The transferred ResNet50 was used to extract deep features of these 2D images. DDAN was employed to extract deep domain-invariant features between the source and target domains, in which the maximum mean discrepancy (MMD) is applied to measure the distance between two different distributions. TCM experiments show that the proposed method significantly outperforms the other three benchmark methods and is more robust under varying working conditions.
A Case Study Demonstrates That the Litter of the Rare Species Cinnamomum migao Composed of Different Tissues Can Affect the Chemical Properties and Microbial Community Diversity in Topsoil
Authors: Xie Yuangui , Liao Xiaofeng , Liu Jiming , Chen Jingzhong
Published: May 2022

The decomposition of litter plays an important role in the return of forest soil nutrients, as well as the growth and productivity of plants. With this study, we aimed to determine the impact of litter mulching on different tissues of Cinnamomum migao, a rare Chinese endemic species. In particular, seeds and pericarp are easily overlooked components of C. migao litter. In this study, we tested control (uncovered litter) and litter (leaf, branch, seed, and pericarp) mulching conditions and conducted a one-year litter decomposition experiment. The enzyme activities of urease enzyme (UE) and invertase enzyme (INV) were significantly improved by litter mulching. Catalase (CAT) enzyme activities in leaf, branch, and seed litter mulching were lower than in the control, whereas CAT activity in pericarp mulching was significantly higher than in the control. Although Mortierella, Cladophialophora, Acidothermus, Sphingomonas, and Burkholderia were the dominant microbes of topsoil in different mulching treatments, there were differences in the number and connectivity of microbial communities, and this change was correlated with soil organic carbon (SOC) and CAT enzyme activity. Compared with leaves and branches, seeds and pericarp as litter are also very important for nutrient return and affect topsoil microbes in C. migao forest, which may be of significance for the growth feedback of C. migao in biennial bearing.