bluuebunny/crossref_metadata_2025 huggingface dataset:
Community Engagement Kelompok Perempuan dalam Budidaya Tanaman Obat Keluarga untuk Penguatan Kesehatan Keluarga
Authors: Setiyaningsih Lian Agustina , Wiyani Wahyu , Prihantono Eko Yuni , Fahmi Muhammad Hanif
Published: August 2022

Pengabdian ini memiliki tujuan untuk memberikan literasi terkait kesehatan keluarga berbasis community engagement dan meningkatkan keterampilan mitra dalam menanam tanaman obat keluarga secara mandiri. Mitra yang dilibatkan adalah kelompok perempuan di Kelurahan Sawojajar. Kegiatan pengabdian ini memakan waktu sekitar tiga bulan dan melibatkan 20 peserta dalam mengikuti penyuluhan dan pelatihan. Metode pengabdian meliputi meliputi observasi, koordinasi dan mobilisasi, penyuluhan, pelatihan pemantauan, panen dan evaluasi. Kegiatan utama pengabdian adalah berupa penyuluhan community engagement dan komunikasi kesehatan, pelatihan menanam tanaman obat bagi keluarga serta melakukan panen. Pengabdian dilakukan Jalan Danau Limboto, Kelurahan Sawojajar Malang, selama tiga bulan mulai bulan Januari hingga Maret 2022. Kelompok perempuan berhasil melakukan panen tanaman obat keluarga yang ditanamanya sendiri dan merencanakan jenis tanaman pada masa tanam selanjutnya. Selain itu, konsep community engagement dan komunikasi kesehatan keluarga dapat dipahami dan diterapkan dalam kehidupan sehari-hari.
Optimalisasi Perancangan Wisata UMKM Terpadu Kendangsari, Surabaya Melalui Digital Branding
Authors: Febriyanti Reny , Efendi Farhan Bachtiar , Arumsari Devinta Nur , Evanthi Ayundha
Published: August 2022

Usaha Mikro Kecil Menengah (UMKM) merupakan salah satu faktor penggerak perekonomian suatu wilayah. Kelurahan Kendangsari memiliki banyak potensi UMKM, namun masih belum dikelola secara optimal untuk bisa memajukan perekonomian di kelurahan tersebut. Optimalisasi tersebut dapat dilakukan dengan merancang pembangunan sebuah destinasi wisata, seperti Wisata UMKM Terpadu Kendangsari dengan berbasis digital branding. Perancangan wisata ini bertujuan untuk memperkuat branding dan pemasaran UMKM yang ada di Kendangsari. Adapun tahapan-tahapan yang dilakukan dalam merancang Wisata UMKM Terpadu Kendangsari, yaitu 1) Pemetaan UMKM, 2) Pelatihan dan Pendampingan UMKM, 3) Pendaftaran Wisata di Google, 4) Pembuatan Akun Instagram Wisata, 5) Pembuatan Alur dan Peta Wisata, dan 6) Pembuatan Video Alur Wisata. Rancangan Wisata UMKM Terpadu Kendangsari juga memberikan manfaat bagi para pelaku UMKM berupa pembuatan akun Instagram bisnis, logo bisnis, dan tips membuat caption yang baik.
Pendampingan Identifikasi Potensi Wisata Dalam Perancangan Grand Design Kampung Wisata Bahari Terpadu Sukolilo Baru Kota Surabaya
Authors: Ardha Dilla Hilmalia , Lutfiani Fauzi Dinda , Wahyu Diyanti Ela , Nur Arumsari Devinta , Evanthi Ayundha
Published: August 2022

Jika ditinjau lebih mendalam, tiap wilayah yang ada di Indonesia memiliki keunikan yang dapat diunggulkan menjadi potensi pariwisata. Sejalan dengan pengembangan sektor pariwisata berbasis masyarakat lokal, Pemerintah Kota Surabaya gencar membangun destinasi wisata sesuai dengan karakteristik tiap wilayah, termasuk di Kelurahan Sukolilo Baru, Kecamatan Bulak, Kota Surabaya. Kawasan Sukolilo Baru ini memiliki bermacam-macam potensi yang lekat dengan laut sehingga dapat dirangkai menjadi suatu wisata bahari terpadu. Tujuan dari kegiatan ini adalah untuk mendukung pengembangan pariwisata di Kelurahan Sukolilo Baru, sehingga masyarakat dapat mengenali potensi-potensi wilayahnya. Kegiatan KKN ini juga memiliki target memberdayakan masyarakat untuk membentuk suatu rancangan atau grand design pariwisata yang cocok dengan karakteristik wilayah Sukolilo Baru. Kegiatan ini terbagi menjadi beberapa tahap yaitu persiapan, pelaksanaan, dan pendampingan. Hasil dari kegiatan ini adalah masyarakat setempat, khususnya pemuda-pemuda di Kawasan Sukolilo Baru dapat mengidentifikasi potensi-potensi wisata dan membentuk suatu rancangan grand design untuk membentuk wisata bahari terpadu Sukolilo Baru.
Análise da saúde mental das profissionais em risco de violência doméstica na atenção básica do município de João Pessoa no contexto da pandemia
Authors: Oliveira Márcya Cândida Casimiro de , Ramos André Luís Belmiro Moreira , Mendonça Gilberto José Góes de , Azevedo Nathália de Oliveira , Tissiani Amanda Apolori , Alves Ianara Fabiana Ramalho Dias , Silva Maria Helena Franklin Domingos da , Pecorelli Domennica Gomes , Coelho Hemílio Fernandes Campos , Deininger Layza de Souza Chaves
Published: August 2022

Objetivo: Analisar a saúde mental das profissionais de saúde em risco de violência doméstica na atenção básica do município de João Pessoa no contexto da pandemia. Métodos: Estudo descritivo, transversal, com abordagem quantitativa. A amostra foi do tipo probabilística e por conglomerados composta por 64 profissionais do sexo feminino selecionadas aleatoriamente. Essas profissionais de saúde foram entrevistadas através de dois instrumentos estruturados na forma de questionário que propunham o rastreamento de sofrimento mental e identificação do risco de sofrer violência doméstica, respectivamente. O estudo foi realizado em 33 Unidades de Saúde da Família em setembro de 2021. Resultados: 70,32% das profissionais de saúde encaixaram-se na categoria de médio risco para violência doméstica e 50% apresentaram indícios de sofrimento mental. O perfil geral das profissionais envolveu mulheres com humor depressivo-ansioso, pensamentos depressivos, sintomas somáticos, decréscimo de energia vital e avaliação negativa sobre si. Contudo, não se observou ideias suicidas, perda do interesse e apetite, características comumente encontradas no humor descrito. Conclusão: A COVID-19 impactou negativamente na saúde mental das profissionais em risco de violência doméstica. Muitas delas, além da sobrecarga laboral, ainda lidam com a violência no contexto familiar, o que influencia substancialmente a sua saúde mental.
ANALYTICAL SOLUTIONS OF (1+1)- DIMENSIONAL DISTRIBUTED LONG WAVE (DLW) EQUATION WITH AUXILIARY EQUATION METHOD
Authors: GÖKTAŞ Ahmet Mücahid , YILMAZ Koray , TAŞBOZAN Orkun
Published: August 2022

In this article, the exact solutions of the (1+1)-dimensional (DLW) equation, a fractional partial differential equation in conformable sense, which is a nonlinear, are given. Furthermore, with the aid of the mathematica program it is seen that the analytical solutions revealed with the auxiliary equation method satisfies the equation.
Spontaneous Browning of White Adipose Tissue Improves Angiogenesis and Reduces Macrophage Infiltration After Fat Grafting in Mice
Authors: Lin Jiayan , Zhu Shaowei , Liao Yunjun , Liang Zhuokai , Quan Yuping , He Yufei , Cai Junrong , Lu Feng
Published: August 2022

Background: Fat grafting is a frequently used technique; however, its survival/ regeneration mechanism is not fully understood. The browning of white adipocytes, a process initiated in response to external stimuli, is the conversion of white to beige adipocytes. The physiologic significance of the browning of adipocytes following transplantation is unclear.

Methods: C57BL/6 mice received 150 mg grafts of inguinal adipose tissue, and then the transplanted fat was harvested and analyzed at different time points to assess the browning process. To verify the role of browning of adipocytes in fat grafting, the recipient mice were allocated to three groups, which were administered CL316243 or SR59230A to stimulate or suppress browning, respectively, or a control group after transplantation.

Results: Browning of the grafts was present in the center of each as early as 7 days post-transplantation. The number of beige cells peaked at day 14 and then decreased gradually until they were almost absent at day 90. The activation of browning resulted in superior angiogenesis, higher expression of the pro-angiogenic molecules vascular endothelial growth factor A (VEGF-A) and fibroblast growth factor 21 (FGF21), fewer macrophages, and ultimately better graft survival (Upregulation, 59.17% ± 6.64% vs. Control, 40.33% ± 4.03%, *p < 0.05), whereas the inhibition of browning led to poor angiogenesis, lower expression of VEGF-A, increased inflammatory macrophages, and poor transplant retention at week 10 (Downregulation, 20.67% ± 3.69% vs. Control, 40.33% ± 4.03%, *p < 0.05).

Conclusion: The browning of WAT following transplantation improves the survival of fat grafts by the promotion of angiogenesis and reducing macrophage.
Occurrence and human health risk assessment of antibiotics in cultured fish from 19 provinces in China
Authors: Tang Yunyu , Lou Xiaoyi , Yang Guangxin , Tian Liangliang , Wang Yuan , Huang Xuanyun
Published: August 2022

The occurrence of antibiotics and potential health risk of 300 cultured fish samples from 19 provinces in China were investigated. The levels of 28 antibiotics (15 fluoroquinolones, 4 tetracyclines, 8 macrolides and rifampin) in 8 fish species were measured through liquid chromatography electrospray tandem mass spectrometry. As a result, 10 antibiotics were detected with an overall detection frequency of 24.3%, and the individual detection frequency of antibiotics ranged from 0.33 to 16.7%. The extremely high concentrations (above 100 µg/kg) of doxycycline and erythromycin were found in the samples. Antibiotics with high detection frequency was noticed in largemouth bass (41.2%), followed by snakehead (34.4%) and bream (31.2%). Specifically, Heilongjiang, Xinjiang, Qinghai and Gansu presented high detection frequency values of more than 60%. Moreover, the highest mean concentration was observed in Shandong, and the concentration covered from 34.8 µg/kg to 410 µg/kg. Despite the high detection frequency and levels of antibiotics were found in samples, ingestion of cultured fish was not significantly related to human health risks in China, according to the calculated estimated daily intakes and hazard quotients. These results provided us the actual levels of antibiotics in cultured fish and human health risk assessment of consuming fishery products.
Prognostic value of post-discharge depression in patients recently hospitalized with acute heart failure
Authors: Li Junlei , Jiang Chao , Liu Rong , Lai Yiwei , Li Li , Zhao Xiaoyan , Wang Xiaofang , Li Ling , Du Xin , Ma Changsheng , Dong Jianzeng
Published: August 2022

Background

Depression is a prevalent comorbidity in patients with heart failure (HF). However, data regarding the prognostic significance of depression during the early post-discharge period in patients hospitalized with acute HF, regardless of left ventricular ejection fraction (LVEF), were scarce.

Methods and results

The Heart Failure Registry of Patient Outcomes (HERO) study is a prospective, multicenter study of patients hospitalized with acute HF in China. At the first follow-up after discharge (median 4.0, interquartile range [IQR]: 2.4–6.1 weeks), depressive symptoms over the past 2 weeks were assessed using the Patient Health Questionnaire-9 (PHQ-9). Of 3,889 patients, 480 (12.3%) patients had depression (PHQ-9 score ≥ 10). A total of 3,456 patients (11.4% with depression) were included in the prospective analysis. After a median follow-up of 47.1 weeks (IQR: 43.9, 49.3) from the first follow-up, 508 (14.7%) patients died, and 1,479 (42.8%) patients experienced a composite event (death or HF rehospitalization). Cox proportional hazards models were used to assess the association of post-discharge depression with adverse events. After adjustment, post-discharge depression was associated with an increased risk of all-cause mortality (hazard ratio [HR] 2.38 [95% confidence interval (CI): 1.93–2.94]; p < 0.001) and the composite event (HR 1.78 [95% CI: 1.55–2.05]; p < 0.001). A per scale point increase in PHQ-9 score (ranging from 0 to 27 points) was associated with a 7.6% increase in all-cause mortality (HR 1.08 [95% CI: 1.06–1.09]; p < 0.001). In the subgroup analysis, the association between depression and the composite event was significantly stronger in relatively younger patients (< 75 vs. ≥ 75 years; p for interaction = 0.011), and the association between depression and all-cause mortality was significantly stronger in patients with preserved ejection fraction than in those with reduced ejection fraction (p for interaction = 0.036).

Conclusion

Post-discharge depression in patients recently hospitalized with acute HF is associated with an increased risk of adverse events, regardless of LVEF. Screening for depressive symptoms during the early post-discharge period may help to better identify high-risk patients and tailor patient management. Further studies are needed to determine how regular depression screening can help improve patient management and clinical outcomes.
Increased proportion of follicular helper T cells is associated with B cell activation and disease severity in IgA nephropathy
Authors: Du Wanshan , Gao Cai-Yue , You Xing , Li Liang , Zhao Zhi-Bin , Fang Mengting , Ye Zhiming , Si Meijun , Lian Zhe-Xiong , Yu Xueqing
Published: August 2022

IgA nephropathy (IgAN) is the most common primary glomerulonephritis, characterized by glomerular deposition of IgA immune complexes, mainly produced by B cells under the regulation of CD4+T cells. However, the alterations of specific CD4+T cell subsets and the mechanism of B cells activation in IgAN remain unclear. Therefore, we aimed to investigate the landscape characteristics and role of CD4+T cells in the progression of IgAN. We identified that the proportion of Th2, Th17 and Tfh (follicular helper T) cells in patients with IgAN was significantly higher than that of healthy controls (P < 0.05). Single-cell RNA sequencing of peripheral blood mononuclear cells (PBMCs) showed that Th cells and B cells in patients with IgAN were more activated. Correspondingly, multiplex immunohistochemistry staining of renal biopsy showed increased infiltration of CD4+T and B cells in the kidneys of patients with IgAN. The degree of infiltration was positively correlated with the degree of renal damage. Interestingly, the proportion of Tfh cells in peripheral blood was positively correlated with the severity of proteinuria. Moreover, the proximity position of Tfh cells and B cells suggested that cell-cell interactions between Tfh and B cells were happening in situ. Intercellular communication analysis also showed enhanced interaction between Tfh cells and B cells in IgAN. Our findings suggested that Tfh cells of patients possibly contributed to the progression of IgAN by activating B cells via cell-cell interactions and TNFSF14-TNFRSF14 may be an underlying signaling pathway.
Pro-inflammatory cytokines and leukocyte integrins associated with chronic neuropathic pain in traumatic and inflammatory neuropathies: Initial observations and hypotheses
Authors: Dong Chaoling , Ubogu Eroboghene E.
Published: August 2022

Leukocyte infiltration and persistence within peripheral nerves have been implicated in chronic nociception pathogenesis in murine peripheral neuropathy models. Endoneurial cytokine and chemokine expression contribute to leukocyte infiltration and maintenance of a pro-inflammatory state that delays peripheral nerve recovery and promotes chronic pain behaviors in these mice. However, there has been a failure to translate murine model data into safe and effective treatments for chronic neuropathic pain in peripheral neuropathy patients, or develop reliable biomarkers that may help diagnose or determine treatment responses in affected patients. Initial work showed that persistent sciatic nerve CD11b+ CD45+ leukocyte infiltration was associated with disease severity in three mouse models of inflammatory and traumatic peripheral neuropathies, implying a direct contributing role in disease pathogenesis. In support of this, CD11b+ leukocytes were also seen in the sural nerve biopsies of chronic neuropathic pain patients with three different peripheral neuropathies. Systemic CD11b antagonism using a validated function-neutralizing monoclonal antibody effectively treated chronic nociception following unilateral sciatic nerve crush injury (a representative traumatic neuropathy model associated with axonal degeneration and increased blood-nerve barrier permeability) and does not cause drug addiction behaviors in adult mice. These data suggest that CD11b could be an effective molecular target for chronic neuropathic pain treatment in inflammatory and traumatic peripheral neuropathies. Despite known murine peripheral neuropathy model limitations, our initial work suggests that early expression of pro-inflammatory cytokines, such as tissue inhibitor of metalloproteinases-1 may predict subsequent chronic nociception development following unilateral sciatic nerve crush injury. Studies aligning animal model investigation with observational data from well-characterized human peripheral neuropathies, including transcriptomics and proteomics, as well as animal model studies using a human clinical trial design should foster the identification of clinically relevant biomarkers and effective targeted treatments with limited addiction potential for chronic neuropathic pain in peripheral neuropathy patients.
CD28 and chemokine receptors: Signalling amplifiers at the immunological synapse
Authors: Molon Barbara , Liboni Cristina , Viola Antonella
Published: August 2022

T cells are master regulators of the immune response tuning, among others, B cells, macrophages and NK cells. To exert their functions requiring high sensibility and specificity, T cells need to integrate different stimuli from the surrounding microenvironment. A finely tuned signalling compartmentalization orchestrated in dynamic platforms is an essential requirement for the proper and efficient response of these cells to distinct triggers. During years, several studies have depicted the pivotal role of the cytoskeleton and lipid microdomains in controlling signalling compartmentalization during T cell activation and functions. Here, we discuss mechanisms responsible for signalling amplification and compartmentalization in T cell activation, focusing on the role of CD28, chemokine receptors and the actin cytoskeleton. We also take into account the detrimental effect of mutations carried by distinct signalling proteins giving rise to syndromes characterized by defects in T cell functionality.
The overlooked problem among surgical patients: Preoperative anxiety at Ethiopian University Hospital
Authors: Woldegerima Berhe Yophtahe , Belayneh Melkie Tadesse , Fitiwi Lema Girmay , Getnet Marye , Chekol Wubie Birlie
Published: August 2022

Introduction

Anxiety was repeatedly reported as the worst aspect of the perioperative time. The objective of this study was to assess the prevalence of preoperative anxiety among adult surgical patients at University of Gondar Comprehensive Specialized Hospital (UoGCSH), Northwest Ethiopia.

Methodology

A hospital-based cross-sectional study was conducted among surgical patients at the university hospital. After obtaining ethical approval, 407 surgical patients were approached during the preoperative period. Preoperative anxiety was assessed by State-Trait Anxiety Inventory. The association between variables was determined by using binary logistic regression analysis. Strength of association was described in adjusted odds ratio (AOR), and a p-value < 0.05 at 95% confidence interval was considered statistically significant.

Results

A total of 400 patients were included in this study with a 98.3% response rate. Preoperative anxiety was observed among 237 (59.3%) patients, and the median (IQR) STAI score was 50 (40–56.7); age, ≥ 60 years (AOR: 5.7, CI: 1.6–20.4, P: 0.007); emergency surgery (AOR: 2.5, CI: 1.3–4.7, P: 0.005); preoperative pain (AOR: 2.6, CI: 1.2–5.4, P: 0.005); and rural residency (AOR: 1.8, CI: 1.1–2.9, P: 0.031) were found significantly associated with preoperative anxiety.

Conclusion

The prevalence of preoperative anxiety among surgical patients was high. Older age (≥ 60 years), emergency surgery, preoperative pain, and rural residency were found significantly associated with preoperative anxiety. Assessment for preoperative anxiety should be a routine component of preoperative assessment of both elective and emergency surgical patients. Preoperative pain should be appropriately managed as it can help to reduce preoperative anxiety. Optimal anxiety reduction methods should be investigated and implemented in the hospital.
Function matters: Coronavirus cross-binding antibodies do not cross-neutralize
Authors: Farcet Maria R. , Schwaiger Julia , Karbiener Michael , Kreil Thomas R.
Published: August 2022

Background

During the current pandemic, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) neutralization capacity of the immunoglobulin (IG) supply has changed from undetectable for lots manufactured from plasma collected before the pandemic, to now highly potent.

Objective

As antibodies induced by exposure to or vaccination against coronaviruses were shown to be cross-coronavirus reactive, it was of interest to understand whether SARS-CoV-2 neutralizing antibodies would result in increased functional IG potency also against seasonal coronaviruses.

Methods

IG lots from US plasma collected before SARS-CoV-2 emerged and collected during the pandemic were analyzed by live virus neutralization assay for SARS-CoV-2 and seasonal human coronaviruses (HCoVs) NL63 and OC43 neutralizing antibody content.

Results

Pre-pandemic IG showed no SARS-CoV-2 neutralizing antibody titers. However, IG lots produced from plasma of post-coronavirus disease 2019 (COVID-19) individuals exhibited robust anti-SARS-CoV-2 potency (1,267 IU/ml) which further increased ~4-fold in pandemic IG lots reaching a mean titer of 5,122 IU/ml. Nonetheless, neutralizing antibody potencies to the HCoVs NL63 and OC43 remained stable over this period, i.e., have not increased correspondingly.

Conclusion

The present results show that cross-coronavirus-reactive antibodies are not cross-neutralizing, i.e., SARS-CoV-2 antibodies do not neutralize seasonal coronaviruses NL63 and OC43.
Performance of quantitative measurements in [18F]fluorocholine positron emission tomography/computed tomography for parathyroid imaging (P2TH study)
Authors: Jacquet-Francillon Nicolas , Morelec Isabelle , Germain Natacha , Prades Jean-Michel , Habouzit Vincent , Mariat Christophe , Bonnefoy Pierre-Benoit , Prevot Nathalie
Published: August 2022

Objective

[18F]Fluorocholine positron emission tomography/computed tomography (PET/CT) is used frequently in addition to [99mTc]Tc-Sestamibi scintigraphy and ultrasonography for the location of hyperfunctioning parathyroid glands. The aim of this study is to evaluate the performance of quantitative criteria in [18F]fluorocholine PET/CT for localization of hyperfunctioning parathyroid glands. The secondary objective is to highlight a correlation between the detection rate of [18F]fluorocholine PET/CT and serum parathyroid hormone (PTH) level.

Materials and methods

In two academic centers, we retrospectively included patients with biological hyperparathyroidism (HPT) and who had [18F]fluorocholine PET/CT. After a visual analysis, to measure the overall performance of [18F]fluorocholine PET/CT, a blind reading was carried out with standardized measurements of maximum standardized uptake value (SUVmax), liver ratio, thyroid ratio, and size ratio. We analyzed the quantitative criteria of [18F]fluorocholine PET/CT compared to the histological results, in particular to identify differences between adenomas and hyperplasias. We compared the performance of each quantitative criterion to the overall sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of [18F]fluorocholine PET/CT. The detection rate of hyperfunctioning parathyroid glands was calculated in subgroups of serum PTH level.

Results

The quantitative criteria in [18F]fluorocholine PET/CT were measured for 120 patients (135 lesions). The areas under the receiver operating characteristic (ROC) curve representing SUVmaxand liver ratio were significantly increased. The optimal cut-off values represented by the maximum Youden index was >4.12 for SUVmaxand >27.4 for liver ratio. Beyond certain threshold values of SUVmax(>4.12) or liver ratio (>38.1), all the lesions were histologically proven adenomas. SUVmaxand liver ratio were significantly higher for adenomas than for hyperplasias and differential diagnosis (p= 0.0085 andp= 0.0002). The positivity of [18F]fluorocholine PET/CT was correlated with PTH level. Detection rates were 55.56, 75.56, and 87.5%, respectively, for serum PTH < 70, 70 to 120, and >120 ng/ml.

Conclusion

Semi-quantitative measurements (SUVmaxand liver ratio) should be considered as additional tools in interpretation of [18F]fluorocholine PET/CT. These quantitative parameters have lower overall performance but higher specificity than overall visual analysis in identifying an adenoma. Above certain threshold values, all lesions are adenomas. [18F]fluorocholine PET/CT confirms excellent performance for the detection of hyperfunctional parathyroids. For serum PTH levels < 70 ng/ml, the detection rate of [18F]fluorocholine PET/CT is strongly decreased.
Altered functional connectivity of cerebellar dentate nucleus in peak-dose dyskinesia in Parkinson’s disease
Authors: Zhang Heng , Wang Lina , Gan Caiting , Cao Xingyue , Ji Min , Sun Huimin , Yuan Yongsheng , Zhang Kezhong
Published: August 2022

The cerebellum is associated with the emergence of levodopa-induced dyskinesia (LID) in Parkinson’s disease (PD), yet the neural mechanism remains obscure. Our aim was to ascertain the role of functional connectivity (FC) patterns of the cerebellar dentate nucleus (DN) in the pathogenesis of peak-dose dyskinesia in PD. Twenty-three peak-dose dyskinetic PD patients, 27 non-dyskinetic PD patients, and 36 healthy controls (HCs) were enrolled and underwent T1-weighted and resting-state functional magnetic resonance imaging (rs-fMRI) scans after dopaminergic medication intake. We selected left and right DN as the regions of interest and then employed voxel-wise FC analysis and voxel-based morphometry analysis (VBM). The correlations between the altered FC pattern and clinical scores were also examined. Finally, receiver operating characteristic (ROC) curve analysis was performed to assess the potential of DN FC measures as a feature of peak-dose dyskinesia in PD. Dyskinetic PD patients showed excessively increased FC between the left DN and right putamen compared with the non-dyskinetic. When compared with controls, dyskinetic PD patients mainly exhibited increased FC between left DN and bilateral putamen, left paracentral lobule, right postcentral gyrus, and supplementary motor area. Additionally, non-dyskinetic PD patients displayed increased FC between left DN and left precentral gyrus and right paracentral lobule compared with controls. Meanwhile, increased FC between DN (left/right) and ipsilateral cerebellum lobule VIII was observed in both PD subgroups. However, no corresponding alteration in gray matter volume (GMV) was found. Further, a positive correlation between the z-FC values of left DN-right putamen and the Unified Dyskinesia Rating Scale (UDysRS) was confirmed in dyskinetic PD patients. Notably, ROC curve analyses revealed that the z-FC values of left DN-right putamen could be a potential neuroimaging feature identifying dyskinetic PD patients. Our findings demonstrated that the excessively strengthened connectivity of DN-putamen might contribute to the pathophysiological mechanisms of peak-dose dyskinesia in PD.
Reliability and validity analysis of personality assessment model based on gait video
Authors: Wen Yeye , Li Baobin , Chen Deyuan , Zhu Tingshao
Published: August 2022

Personality affects an individual’s academic achievements, occupational tendencies, marriage quality and physical health, so more convenient and objective personality assessment methods are needed. Gait is a natural, stable, and easy-to-observe body movement that is closely related to personality. The purpose of this paper is to propose a personality assessment model based on gait video and evaluate the reliability and validity of the multidimensional model. This study recruited 152 participants and used cameras to record their gait videos. Each participant completed a 44-item Big Five Inventory (BFI-44) assessment. We constructed diverse static and dynamic time-frequency features based on gait skeleton coordinates, interframe differences, distances between joints, angles between joints, and wavelet decomposition coefficient arrays. We established multidimensional personality trait assessment models through machine learning algorithms and evaluated the criterion validity, split-half reliability, convergent validity, and discriminant validity of these models. The results showed that the reliability and validity of the Gaussian process regression (GPR) and linear regression (LR) models were best. The mean values of their criterion validity were 0.478 and 0.508, respectively, and the mean values of their split-half reliability were all greater than 0.8. In the formed multitrait-multimethod matrix, these methods also had higher convergent and discriminative validity. The proposed approach shows that gait video can be effectively used to evaluate personality traits, providing a new idea for the formation of convenient and non-invasive personality assessment methods.
Differentiation of Parkinson’s disease and Parkinsonism predominant multiple system atrophy in early stage by morphometrics in susceptibility weighted imaging
Authors: Ren Qingguo , Wang Yihua , Xia Xiaona , Zhang Jianyuan , Zhao Cuiping , Meng Xiangshui
Published: August 2022

Background and purpose

We previously established a radiological protocol to discriminate multiple system atrophy-parkinsonian subtype (MSA-P) from Parkinson’s disease (PD). However, we do not know if it can differentiate early stage disease. This study aimed to investigate whether the morphological and intensity changes in susceptibility weighted imaging (SWI) of the lentiform nucleus (LN) could discriminate MSA-P from PD at early stages.

Methods

We retrospectively enrolled patients with MSA-P, PD and sex- and age-matched controls whose brain MRI included SWI, between January 2015 and July 2020 at the Movement Disorder Center. Two specialists at the center reviewed the medical records and made the final diagnosis, and two experienced neuroradiologists performed MRI analysis, based on a defined and revised protocol for conducting morphological measurements of the LN and signal intensity.

Results

Nineteen patients with MSA-P and 19 patients with PD, with less than 2 years of disease duration, and 19 control individuals were enrolled in this study. We found that patients with MSA- P presented significantly decreased size in the short line (SL) and corrected short line (cSL), ratio of the SL to the long line (SLLr) and corrected SLLr (cSLLr) of the LN, increased standard deviation of signal intensity (SIsd_LN, cSIsd_LN) compared to patients with PD and controls (P < 0.05). With receiver operating characteristic (ROC) analysis, this finding had a sensitivity of 89.5% and a specificity of 73.7% to distinguish MSA- P from PD.

Conclusion

Compared to PD and controls, patients with MSA-P are characterized by a narrowing morphology of the posterior region of the LN. Quantitative morphological changes provide a reference for clinical auxiliary diagnosis.
Altered effective connectivity in the emotional network induced by immersive virtual reality rehabilitation for post-stroke depression
Authors: Wu Jia-Jia , Zheng Mou-Xiong , Hua Xu-Yun , Wei Dong , Xue Xin , Li Yu-Lin , Xing Xiang-Xin , Ma Jie , Shan Chun-Lei , Xu Jian-Guang
Published: August 2022

Post-stroke depression (PSD) is a serious complication of stroke that significantly restricts rehabilitation. The use of immersive virtual reality for stroke survivors is promising. Herein, we investigated the effects of a novel immersive virtual reality training system on PSD and explored induced effective connectivity alterations in emotional networks using multivariate Granger causality analysis (GCA). Forty-four patients with PSD were equally allocated into an immersive-virtual reality group and a control group. In addition to their usual rehabilitation treatments, the participants in the immersive-virtual reality group participated in an immersive-virtual reality rehabilitation program, while the patients in the control group received 2D virtual reality rehabilitation training. The Hamilton Depression Rating Scale, modified Barthel Index (MBI), and resting-state functional magnetic resonance imaging (rsfMRI) data were collected before and after a 4-week intervention. rsfMRI data were analyzed using multivariate GCA. We found that the immersive virtual reality training was more effective in improving depression in patients with PSD but had no statistically significant improvement in MBI scores compared to the control group. The GCA showed that the following causal connectivities were strengthened after immersive virtual reality training: from the amygdala, insula, middle temporal gyrus, and caudate nucleus to the dorsolateral prefrontal cortex; from the insula to the medial prefrontal cortex; and from the thalamus to the posterior superior temporal sulcus. These causal connectivities were weakened after treatment in the control group. Our results indicated the neurotherapeutic use of immersive virtual reality rehabilitation as an effective non-pharmacological intervention for PSD; the alteration of causal connectivity in emotional networks might constitute the neural mechanisms underlying immersive-virtual reality rehabilitation in PSD.
Therapeutic Uses and Pharmacological Properties of Shallot (Allium ascalonicum): A Systematic Review
Authors: Moldovan Cadmiel , Frumuzachi Oleg , Babotă Mihai , Barros Lillian , Mocan Andrei , Carradori Simone , Crişan Gianina
Published: August 2022

Background

Shallot (Allium ascalonicumL.) is a traditional plant species used throughout the world both for culinary purposes and as a folk remedy. To date (i.e., April 2022), there is no report on the main pharmacological activities exerted by shallot preparations and/or extracts.

Scope and Approach

The aim of this study was to comprehensively review the pharmacological activities exerted by shallot, with rigorous inclusion and exclusion criteria based on the scientific rigor of studies. Prisma guidelines were followed to perform the literature search.

Key Findings and Conclusions

The literature search yielded 2,410 articles of which 116 passed the required rigorous criteria for inclusion in this review. The extracts exert a potent antioxidant activity bothin vitroandin vivo, as well as a strong inhibitory capacity on various pathogens with relevant implications for public health. Moreover, shallot can be used as adjuvant therapy in cardiovascular diseases, diabetes, cancer prevention, and other non-communicable diseases associated with inflammatory and oxidative pathways. Future studies investigating the chemical composition of this species, as well as the molecular mechanisms involved in the empirically observed pharmacological actions are required.
Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
Authors: Yue Fangfang , Zhang Jinrui , Xu Jiaxin , Niu Tengfei , Lü Xin , Liu Manshun
Published: August 2022

Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study. However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples. Therefore, the present study focused to unveil the proposed problem. Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis. In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content. Moreover, the key points during the application of phenol-sulfuric acid method were suggested. This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
Portulaca oleracea polysaccharides reduce serum lipid levels in aging rats by modulating intestinal microbiota and metabolites
Authors: Fu Qiang , Huang Hui , Ding Aiwen , Yu Ziqi , Huang Yuping , Fu Guiping , Huang Yushan , Huang Xiaoliu
Published: August 2022

Metabolic diseases characterized by dyslipidemia are common health problems for elderly populations. Dietary fiber intake is inversely associated with the risk of dyslipidemia. This study investigated the effects of Portulaca oleracea polysaccharide (POP) on the intestinal microbiota and its metabolites in aging rats using 16S rRNA sequencing and metabolomics techniques. Our results showed that POPs reduced the ratio of Firmicutes/Bacteroidetes (F/B), relative abundance of Fusobacteria, and levels of triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and gamma-glutamyl transferase (γ-GT) in the serum of aging rats. POP supplementation also reduced 5beta-cholestane-3alpha,7alpha,12alpha,25-tetrol, and vaccenic acid concentrations in lipids and lipoid-like molecules, while soyasapogenol E and monoacylglycerol (MG) (24:0/0:0/0:0) levels increased. This study demonstrated that POP’s beneficial effects on lipid levels in aging rats might be partially attributable to the modification of gut microbiota and related metabolites.
Whole genome analysis reveals the genomic complexity in metastatic cutaneous squamous cell carcinoma
Authors: Thind Amarinder Singh , Ashford Bruce , Strbenac Dario , Mitchell Jenny , Lee Jenny , Mueller Simon A. , Minaei Elahe , Perry Jay R. , Ch’ng Sydney , Iyer N. Gopalakrishna , Clark Jonathan R. , Gupta Ruta , Ranson Marie
Published: August 2022

Metastatic cutaneous squamous cell carcinoma (CSCC) is a highly morbid disease requiring radical surgery and adjuvant therapy, which is associated with a poor prognosis. Yet, compared to other advanced malignancies, relatively little is known of the genomic landscape of metastatic CSCC. We have previously reported the mutational signatures and mutational patterns of CCCTC-binding factor (CTCF) regions in metastatic CSCC. However, many other genomic components (indel signatures, non-coding drivers, and structural variants) of metastatic CSCC have not been reported. To this end, we performed whole genome sequencing on lymph node metastases and blood DNA from 25 CSCC patients with regional metastases of the head and neck. We designed a multifaceted computational analysis at the whole genome level to provide a more comprehensive perspective of the genomic landscape of metastatic CSCC. In the non-coding genome, 3′ untranslated region (3′UTR) regions of EVC (48% of specimens), PPP1R1A (48% of specimens), and ABCA4 (20% of specimens) along with the tumor-suppressing long non-coding RNA (lncRNA) LINC01003 (64% of specimens) were significantly functionally altered (Q-value < 0.05) and represent potential non-coding biomarkers of CSCC. Recurrent copy number loss in the tumor suppressor gene PTPRD was observed. Gene amplification was much less frequent, and few genes were recurrently amplified. Single nucleotide variants driver analyses from three tools confirmed TP53 and CDKN2A as recurrently mutated genes but also identified C9 as a potential novel driver in this disease. Furthermore, indel signature analysis highlighted the dominance of ID signature 13 (ID13) followed by ID8 and ID9. ID9 has previously been shown to have no association with skin melanoma, unlike ID13 and ID8, suggesting a novel pattern of indel variation in metastatic CSCC. The enrichment analysis of various genetically altered candidates shows enrichment of “TGF-beta regulation of extracellular matrix” and “cell cycle G1 to S check points.” These enriched terms are associated with genetic instability, cell proliferation, and migration as mechanisms of genomic drivers of metastatic CSCC.
Patient-Derived Organoid Facilitating Personalized Medicine in Gastrointestinal Stromal Tumor With Liver Metastasis: A Case Report
Authors: Cao Ying , Zhang Xi , Chen Qianyun , Rao Xi , Qiu Enming , Wu Gang , Lin Yu , Zeng Ziqi , Zheng Bin , Li Zhou , Cai Zhai , Wang Huaiming , Han Shuai
Published: August 2022

The gastrointestinal stromal tumors (GIST) are a rare gastrointestinal tract malignancy. The two primary mutation sites are found in KIT and platelet-derived growth factor receptor-α (PDGFR-α) genes. The current study reports on a point mutation within the exon 11 of KIT, named KIT p.V560E. Patient-derived organoids (PDOs) are potential 3D in vitro models of tissues that can be used to identify sensitivity toward specific targets in patients with tumors and allow for personalized medicine when drugs specific for newly identified genetic locus mutations are not yet available. This study describes a 68-year-old patient who complained of diffused abdominal pain and intermittent melena lasting more than 10 days. He has no other gastrointestinal abnormalities, prior abdominal surgery, or related family history. Surgery was conducted first to remove the lesions and ascertain the disease through histology and immunohistochemical stains of the mass. Immunohistochemistry revealed that the tumor was positive for CD117 and Dog-1. Based on the above findings, he was diagnosed with GISTs. Gene detection analysis and organoid culture were then performed to verify clinical decisions. KIT p.V560E and the reduced number of RB1 copies were identified as two obvious mutations, so the patient was administrated first-line treatment of imatinib 400 mg/d. However, progressive disease prompted us to switch to sunitinib, and his condition gradually improved. Meanwhile, organoid culture showed sensitivity to sunitinib and tolerance to imatinib with half-maximal inhibitory concentration (IC50) values of 0.89 and >20, respectively. In summary, to the best of our knowledge, this is the first time that the established organoid culture indicated that the GISTs organoid could identify the sensitivity to target therapies and facilitate individual-based treatment.
Case Report: A rare case of familial lung cancer requiring pneumonectomy in three male siblings
Authors: Kaprin Andrey , Pikin Oleg , Ryabov Andrey , Aleksandrov Oleg , Toneev Evgeniy , Lubchenko Ludmila , Zelenova Ekaterina
Published: August 2022

Lung cancer is a disease with a unique genetic pattern and is occasionally related to hereditary syndromes such as Lynch, Louis–Bar, and Li–Fraumeni. In some patients, germinal mutations may be discovered in combination with somatic alterations. For instance, Li–Fraumeni syndrome often reveals a mixture of TP53 and EGFR mutations. The development of new target therapies necessitates an extensive search for new pathogenic mutations. In this article, we present a rare case report of lung cancer, requiring a pneumonectomy, in three sibling brothers.
Research trends of artificial intelligence in pancreatic cancer: a bibliometric analysis
Authors: Yin Hua , Zhang Feixiong , Yang Xiaoli , Meng Xiangkun , Miao Yu , Noor Hussain Muhammad Saad , Yang Li , Li Zhaoshen
Published: August 2022

Purpose

We evaluated the related research on artificial intelligence (AI) in pancreatic cancer (PC) through bibliometrics analysis and explored the research hotspots and current status from 1997 to 2021.

Methods

Publications related to AI in PC were retrieved from the Web of Science Core Collection (WoSCC) during 1997-2021. Bibliometrix package of R software 4.0.3 and VOSviewer were used to bibliometrics analysis.

Results

A total of 587 publications in this field were retrieved from WoSCC database. After 2018, the number of publications grew rapidly. The United States and Johns Hopkins University were the most influential country and institution, respectively. A total of 2805 keywords were investigated, 81 of which appeared more than 10 times. Co-occurrence analysis categorized these keywords into five types of clusters: (1) AI in biology of PC, (2) AI in pathology and radiology of PC, (3) AI in the therapy of PC, (4) AI in risk assessment of PC and (5) AI in endoscopic ultrasonography (EUS) of PC. Trend topics and thematic maps show that keywords " diagnosis ", “survival”, “classification”, and “management” are the research hotspots in this field.

Conclusion

The research related to AI in pancreatic cancer is still in the initial stage. Currently, AI is widely studied in biology, diagnosis, treatment, risk assessment, and EUS of pancreatic cancer. This bibliometrics study provided an insight into AI in PC research and helped researchers identify new research orientations.
Association of early severe thrombocytopenia and platelet course with in-hospital mortality in critically ill children
Authors: Lu Yan , Ren Chaoxiang , Guo Haoyang
Published: August 2022

This study aimed to assess the association of early severe thrombocytopenia and platelet course with in-hospital mortality in critically ill children. Data of critically ill children in this study were obtained from the Pediatric Intensive Care Database. Patients with and without severe thrombocytopenia were adjusted for covariates using propensity score matching (PSM) to ensure the robustness of the results. Univariate and multivariate logistic regression analyses were performed on the original and PSM cohorts, respectively. Results are presented as odds ratios (ORs) with 95% confidence intervals (95% CI). In studies of the platelet course, logistic regression analysis was used to assess the effect of different degrees of recovery on in-hospital mortality in critically ill children with early severe thrombocytopenia. The study included 4,848 critically ill children, of whom 450 with early severe thrombocytopenia were matched to 450 without early severe thrombocytopenia. Univariate and multivariate logistic regression results showed that early severe thrombocytopenia was an independent risk factor for in-hospital mortality in critically ill children in both the original and PSM groups. In addition, the study results of platelet course showed that the recovery of platelet count to ≥150 × 109/L in the short term was a protective factor for the prognosis of patients (OR, 0.301; 95% CI, 0.139–0.648, P = 0.002). Our study revealed that early severe thrombocytopenia is an independent risk factor for in-hospital mortality in critically ill children. In addition, in-hospital mortality was significantly reduced in children with early severe thrombocytopenia, whose platelet count returned to normal levels in the short term.