bluuebunny/crossref_metadata_2025 huggingface dataset:
The vision of regional history in the Soviet textbooks of the socio-humanitarian block in 1920-1930
Authors: Bezpalko Bohdan , Khokhlov Denys
Published: September 2023

The article examines the peculiarities of forming the vision of regional history, which was presented in the textbooks of the socio-humanitarian block of the 1920s-1930s. The historiography of the issue is analyzed. Examining the vision of regional history in Soviet textbooks, the author touched on the problem of the Soviet school system functioning in general and the history lesson in particular. The main textbooks of the specified period, which are representative of the presented issues were also analyzed, because they concern secondary general schools, that is, those children who were the basis of the school education system. The article reveals the spatial vision presented in the work of M. Yavorsky "A Brief History of Ukraine" – the main textbook of that period. Among the regions distinguished by the Soviet information policy were: Ukraine as part of the USSR; Naddniprianshchyna with the main city Kyiv, Galicia, Volyn and Zakarpattya (Transcarpathia). Also, the peculiarity of the vision of regional history, which was formed at the beginning of the next decade, is revealed. It consisted in shifting the vector from the west to the eastern spaces – Russian lands. Kyiv as a regional center receded into the background, giving way to St. Petersburg and Moscow. The specified vision deepened even more at the end of the 1930s. Textbooks of that period almost did not describe Ukrainian lands, dividing them into two artificial regions, "Eastern and Western" Ukraine. Instead, the regional vision spread to Central Asia, Kazakhstan and the Caucasus.
The influence of the Tiflis entourage on the formation of Mykhailo Hrushevsky's personality
Authors: Romantsova Natalya
Published: September 2023

The proposed study analyzes issues related to the formation of M. Hrushevsky's personality in Tiflis (Tbilisi) - the cultural center of the South Caucasus, where he spent his youth, studying in a gymnasium. The peculiarities of this region were considered and it was clarified how the entourage of Tiflis influenced the personality of young Mykhaylo. This, in the opinion of the article’s author, is important for a full understanding on the historical significance of the figure of Hrushevsky. In particular, the author draws attention to the influence of the Tiflis entourage in the matter of establishing the personality markers of young Mykhailo – the beginning of his intellectual biography formation, the way of establishing his Ukrainian patriotism, interests in Ukrainian studies, history of Ukraine, literature, folklore. Analysis of M. Hrushevsky's life gymnasium period permits to reveal the combination of creativity, innovation, unusual efficiency, ambition, and perseverance in his activities. Theoretical studies of authoritative foreign and Ukrainian scientists F. Brodel, L. Kjell, D. Ziegler, Ya. Vermenych, P. R. Magochy, S. Maksymenko and others are included in the analysis of the chosen subject. According to the interdisciplinary approach, the article combines elements of regional history, social psychology, and sociology.
Exploration of the Protective Effects of Aronia Melanocarpa Extract in an in vitro Model of Parkinson’s Disease
Authors: Mijatović Ela , Harej Hrkać Anja , Gržeta Nika , Pilipović Kristina
Published: September 2023

Aim: In this study, we aimed to explore whether the Aronia melanocarpa (chokeberry) extract pre-treatment protects primary mouse astrocytes in an in vitro model of Parkinson's disease (PD) induced by the application of mitochondrial toxin 6-hydroxydopamine (OHDA) and if this effect was related to its antioxidant properties. Material and methods: Experiments were performed on primary mouse astrocytes that were exposed for 24 h to different concentrations of chokeberry extract (10, 50 or 100 µg/mL). OHDA was added to the cell culture medium for the next 72 h. Cell culture media were prepared for the lactate dehydrogenase (LDH) cell cytotoxicity assay and the cell lysates for the western blot analyses. Results: We determined that the pre-treatment of astrocytes with different concentrations of chokeberry extract alone does not significantly affect the LDH activity in the cell media samples. Application of OHDA, however, significantly increased the LDH activity while the pre-treatment of cells with chokeberry extract in concentrations of 50 and 100 μg/mL decreased the intensity of cell damage. Neither OHDA application, nor aronia pre-treatment affected the expression level of antioxidant enzyme superoxide dismutase 1, and the OHDA-induced decrease in glutathione peroxidase 1 protein expression remained unchanged by the tested antioxidant application. Conclusions: This study points to possible protective effects of chokeberry extract in an in vitro model of PD. The exact mechanism of the protective effect is still unclear and could be the subject of further research.
Nove epidemiološke osobitosti hepatitisa A - iskustva Kliničkog bolničkog centra Rijeka u dvanaestogodišnjem razdoblju
Authors: Ferenčić Valentina , Slavuljica Irena
Published: September 2023

Cilj: Incidencija hepatitisa A u našoj zemlji, slično ostalim razvijenim zemljama, niska je zahvaljujući poboljšanju socioekonomskog standarda i higijenskih uvjeta. U razvijenim zemljama svijeta posljednje desetljeće opisane su epidemije hepatitisa A vezane uz „novi”, spolni put prijenosa, poglavito u MSM populaciji (engl. Men Sex Men; MSM) i u osoba s drugim spolno prenosivim infekcijama. Budući da u razvijenim zemljama od hepatitisa A češće obolijevaju osobe odrasle dobi, učestalost komplikacija veća je u odnosu na nerazvijene zemlje gdje je hepatitis A bolest dječje dobi, što je poznato kao „paradoksalni rizik”. Svrha je ovog rada opisati epidemiološke osobitosti i kliničku sliku bolesnika s hepatitisom A u proteklom dvanaestogodišnjem razdoblju. Ispitanici i metode: Istraživanjem je obuhvaćen 21 bolesnik s hepatitisom A liječen u Klinici za infektivne bolesti Kliničkog bolničkog centra Rijeka u razdoblju od 2010. do 2021. godine. Rezultati: Najveći dio oboljelih osoba bio je srednje životne dobi (40 - 49 godina). U razdoblju do 2010. do 2016. godine liječen je 0 - 1 bolesnik s hepatitisom A godišnje, dok je 2017. i 2018. godine liječeno 16 bolesnika i 2019. dva bolesnika. Godine 2020. i 2021., u vrijeme epidemije COVID-19, nismo liječili oboljele od hepatitisa A. Većinu oboljelih činili su muškarci. Uz fekooralni put prijenosa glavni put prijenosa infekcije bio je spolni put u MSM populaciji. Tijek bolesti bio je blag. Zaključci: Epidemiološke značajke bolesnika s hepatitisom A obuhvaćenih studijom zrcale nove epidemiološke trendove hepatitisa A u razvijenim europskim zemljama - s najvećim brojem oboljelih u odrasloj dobi i „novim”, spolnim putem prijenosa infekcije.
Breast Cancers Secreting Sialyl-fibronectin Are Less Likely to Cause Epithelial-mesenchymal Transition and Have Good Prognoses
Authors: TAKEYAMA HIROSHI , MANOME YOSHINOBU
Published: September 2023

Background/Aim: Elevated blood fibronectin (FN) levels have been observed in various cancers; however, their significance is controversial. We measured sialyl-fibronectin (S-FN), a type of FN secreted by tumor cells in the blood, and investigated whether blood S-FN secretion is associated with cancer malignancy and recurrent metastases. Materials and Methods: We constructed an enzyme-linked immunosorbent assay (ELISA) system that recognizes S-FN as an antigen and measured the amount of S-FN secreted into the blood of 89 patients with breast tumors. The relationship between S-FN secretion and prognostic predictors was analyzed. Immunostaining was performed to identify the site of S-FN secretion in the breast tissue. Results: Among the 82 patients, 21 (25.6%, 21/82) and 61 (74.4%, 61/82) were blood S-FN-positive and S-FN-negative, respectively. Regarding prognostic predictors, blood S-FN-positive and S-FN-negative patients showed significant difference in locoregional recurrence (p=0.026), remote metastases (p=0.049), and histological margins (p=0.001). Locoregional recurrence was associated with positive histological margins in S-FN-positive patients. However, remote metastases were associated with N-factor and histological classification (HC) in S-FN-negative patients. Furthermore, S-FN particles were detected in the cytoplasm of breast cancer cells through immunostaining. After the onset of recurrent metastases, two S-FN-positive and six S-FN-negative patients received anticancer drug treatment; however, further progression was observed in five S-FN-negative patients. Conclusion: S-FN-positive patients are less likely to develop distant metastases, have a better prognosis, and may be less resistant to therapeutic agents than S-FN-negative patients, which contain many epithelial-mesenchymal transition cells.
Optimal Timing of Laparoscopic Cholecystectomy After Conservative Therapy for Acute Cholecystitis
Authors: ENAMI YUTA , AOKI TAKESHI , TOMIOKA KODAI , HIRAI TAKAHITO , SHIBATA HIDEKI , SAITO KAZUHIKO , NAGAISHI SHODAI , TAKANO YOJIRO , SEKI JUNICHI , SHIMADA SHOJI , NAKAHARA KENTA , TAKEHARA YUSUKE , MUKAI SHUMPEI , SAWADA NARUHIKO , ISHIDA FUMIO , KUDO SHIN-EI
Published: September 2023

Background/Aim: According to the Tokyo Guidelines 2018, the operation for acute cholecystitis is recommended to be performed as early as possible. However, there are cases in which early surgeries cannot be performed due to complications of patients or facility conditions, resulting in elective surgery. Hence, we retrospectively analyzed elective surgery cases in this study. Patients and Methods: There were 345 patients who were underwent laparoscopic cholecystectomy (LC) at our hospital from January 2019 to December 2020 in this retrospective study. A total of 83 patients underwent LC more than 3 days after conservative treatment. The elective LC patients were divided into the Early group (4-90 days after onset, n=36) and the Delayed group [91 days or more (13 weeks or more) after onset, n=31], excluding 16 patients who underwent percutaneous transhepatic gallbladder drainage. Results: As for operative time, there was a significant difference between the Delayed and Early groups (91.2 vs. 117 minutes, p=0.0108). And also, there was a significant difference in the postoperative hospital stay, which was significantly shorter in the Delayed group than in the Early group (3.4 vs. 5.9 days, p=0.0436). Although there were no significant differences in either conversion rates or complication rates, both of these were decreasing in the Delayed group. In particular, there were no complications in the Delayed group. Conclusion: When the conservative treatment for acute cholecystitis precedes and precludes urgent/early LC within 3 days, delaying LC for at least 91 days (13 weeks or more) after onset could reduce operative time and postoperative hospital stay. Moreover, there would be no complications after LC, and the rates of conversion during LC may be kept low.
Adding Ginkgo Biloba leaf extract to the diet as an antioxidant and
studying its effect on the productive performance of Ross 308 broilers.
Authors: Taleb Diab Ammar , Al-kinani Ali saleh Hassan
Published: September 2023

The experiment was carried out in the poultry field of the Department
of Animal Production/College of Agriculture/the University of Diyala during the
period from 4/9/2021 to 15/10/2021 to study the effect of adding ginkgo leaf extract to broiler diets as an antioxidant and study its impact on the productive performance of birds. This experiment used 225 unsexed hybrid broiler chicks (age:
one day). The average weight of the chick was (43.62 g.). Firstly, The chicks
were randomly distributed by using five experimental treatments. Each treatment
has three replicates (15 birds/repeat). Experimental transactions are as follows.
T1 = (control treatment) A standard diet without addition is provided, T2 =
standard ratio to which 1 g/kg of Ginkgo Biloba leaf extract is added, T3 =
Standard diet to which 2 g/kg of Ginkgo Biloba leaf extract is added, T4 = a
standard diet to which 3 g/kg of Ginkgo Biloba leaf extract is added, and T5 =
standard ration to which 4 g/kg of Ginkgo Biloba leaf extract is added. The results of the experiment were as follows. The experiment results indicated a significant effect (P≤0.05) when adding different levels of Ginkgo biloba leaf extract to the diets of 308 Ross broilers for 1-42 days. The fourth treatment was superior to the average live body weight during the sixth week and the cumulative
weight gain compared to the control. There is a significant superiority (P≤0.05) in
the rate of cumulative feed consumption, cumulative feed conversion, efficiency
and production index value, where all the addition treatments (1,2,3,4)g/kg feed
excelled compared to the control.
Keywords: Ginkgo biloba leaves Broiler, Growth performance, Antioxidant activity.
Effect of adding Cynara scolymus L. (artichoke) extract to water or
diet on the productive performance of broilers
Authors: Taleb Dhiab Ammar , Jabbar Ali Ali
Published: September 2023

The experiment was carried out in the poultry field of the Department
of Animal Production/College of Agriculture/the University of Diyala from
4/9/2021 to 10/14/2021 to study the effect of adding Cynara scolymus L. )artichoke( extract to the diet of broilers. It was used as an antioxidant to show its effect on birds' productive, physiological and immune performance. In this experiment, 225 unsexed Ross 308 hybrid broiler chicks were used (one day age), with
an initial weight of 44.36 g/chick. These chicks were randomly distributed from
the first day of receiving them to five experimental treatments with three replicates for each treatment (15 birds/repeat). The experimental treatments were: T1
= (control treatment) provided a standard diet free of additives, T2 = Standard ration plus 75 mg/L artichoke extract, T3 = a standard diet supplemented with 150
mg/L artichoke extract, T4 = Standard ration to which 2 g/kg of artichoke extract
is added, and T5 = Standard ration to which 3 g/kg of artichoke extract is added.
The experiment results indicated a significant effect on product traits when adding different artichoke leaf extract powder levels to Ross 308 broiler water and
diet (1-42 days). Generally, treatment T3 was significantly (P<0.05) superior in
the fourth week in recording the highest average live body weight of 3170.90 g,
and treatment T4 was significantly (P<0.05) in the fourth week and recorded the
highest rate of weight gain. Also, treatment T1 in the first week recorded the
highest rate of feed consumption, treatment T2 in the first week recorded the
highest efficiency of food conversion, and treatment T3 significantly (P<0.05)
and recorded the highest rate of productive evidence.
Keywords: broiler, artichoke leaf extract, productive traits
Effects of husk charcoal and watering on the biochemical and physiological properties of coix millet (Coix lacryma jobi L.) during vegetative phase
Authors: Fadillatunnisa Fadillatunnisa , Indradewa Didik , Putra Eka Tarwaca Susila
Published: September 2023

Continuous climate change increases the number of droughts in some areas, thereby affecting agricultural production. Cultivation of coix millet (Jali plant in Indonesian) is profitable because of its high adaptability to drought stress, and its seeds have the potential to be used as food due to their benefits for body health. The application of organic matter, namely husk charcoal, was chosen to increase agricultural production under drought stress. Therefore, this study aimed to determine the effects of husk charcoal on the biochemical and physiological properties of coix millet during vegetative phase under drought stress and to determine the longest watering interval the plant can tolerate. Research was arranged in a split-plot design with three replications. The main plot consisted of three watering intervals (every two, four, and six days). The sub-plots consisted of three doses of husk charcoal, namely 0 g polybag⁻¹ (0 tons ha⁻¹), 32 g polybag⁻¹ (10 tons ha⁻¹), and 64 g polybag⁻¹ (20 tons ha⁻¹). The data were analyzed using analysis of variance (ANOVA) and tested using Duncan's Multiple Distance Test (DMRT) at a significant level of 5%. The results showed that husk charcoal could increase the chlorophyll content in plants experiencing drought stress. The longest watering interval that could be tolerated by coix millet given husk charcoal at the optimal dose (64 g polybag⁻¹) was once every four days.
Effects of ameliorant application on the growth and yield of rice plants (Oryza sativa L.)
Authors: Farhati Arini Alfi , Firnia Dewi , Laila Alfu
Published: September 2023

An ameliorant is a chemical that can be used to improve soil fertility. The improving agents include dolomite and steel slag. Steel slag is a by-product of the steel industry containing CaCO3, SiO2, CaSiO3, and MgO, which can be used to improve the growth and yield of rice plants. Dolomite, on the other hand, is a limestone rock containing MgO and CaO, which is useful in agricultural soils. This study aimed to determine the types and doses of ameliorants affecting the growth and yield of rice plants (Oryza sativa L.). The research was carried out at Sukadana Village, Ciomas Serang-Banten District. A non-factorial Group Randomized Design with seven treatments was employed in this investigation. The treatments include P0 = No treatment (control), P1 = 2 t/ha of dolomite, P2 = 2 t/ha of dolomite + 2.5 t/ha of steel slag, P3 = 1.5 t/ha of steel slag, P4 = 2.5 t/ha of steel slag, P5 = 5 t/ha of steel slag, and P6 = 7.5 t/ha of steel slag. Observation was made on steel slag characterization, plant height, the total number of tillers, the weight of 1000 grains, the amount of grain per clump, and the weight of dry-milled grain. The results showed that applying dolomite at 2 t/ha and steel slag at 2.5 t/ha could increase the weight of 1000 grains of rice, and applying steel slag at 1.5 t/ha could enhance the amount of grain per clump.
Application of NAA and BA to Calotropis gigantea (L.) W.T. Aiton in vitro
Authors: Rahayu Muji , Wulandari Tyas Alivia , Sakya Amalia Tetrani , Setyawati Andriyana , Hartati Sri , Samanhudi Samanhudi
Published: September 2023

Crown flower (Calotropis gigantea (L.)) is a weed plant potentially to be used as raw material for textile fibers yet the utilization is not optimal. Efforts to optimize the utilization of C. gigantea are needed. This research aimed to determine the appropriate concentration of plant growth regulators, including NAA (Naphthalene Acetic Acid) and BA (Benzyl Adenine) to stimulate optimal growth of C. gigantea to be used as the basis for in vitro propagation of C. gigantea. The research was arranged in a completely randomized design (CRD). Explants were planted on Murashige and Skoog (MS) medium with various concentrations of NAA (0; 0.5; 1; 1.5; and 2 ppm) and BA (0; 1; 2; 3; and 4 ppm). Based on the research result, the addition of 3 ppm BA followed by increasing the concentration of NAA to 1.5 ppm could increase the number of C. gigantea shoots, and giving 1 ppm BA followed by adding 1.5 to 2 ppm NAA increased shoot height. The best plantlet response was found in media with 0 ppm NAA and 4 ppm BA, which could produce an optimal shoot height of 5.9 cm and a large number of shoots of 5.67 shoots. Increasing NAA concentration retarded root formation and reduced the root length and number of leaves, while the medium without NAA gave the earliest root emergence of 11 days after planting, the number of leaves of 20 strands, and the longest root of 10.9 cm. Applying a single substance BA did not accelerate all the variables observed.
Effects of different soil management styles and cropping practices on the yield and quality of fava bean (Vicia faba L.)
Authors: Karami Somayeh
Published: September 2023

This research aimed to determine the effects of various soil tillage techniques and the use of organic and chemical fertilizers on the overall yield and quality of fava beans. The study emphasized the significance of fertilizer types and their interaction with soil tillage methods. Although the quantity of grains per pod was notably influenced by the choice of fertilizers, the impact of soil tillage alone was found to be insignificant. Moreover, this study demonstrated that the yield index of fava beans was significantly influenced by fertilizer sources, emphasizing the importance of proper nitrogen managements. These findings provide valuable insights for enhancing crop production and quality through optimized soil tillage practices and the use of organic and chemical fertilizers. Through an examination of both the measurable and qualitative elements of fava bean cultivation, this study offers valuable knowledge on how to optimize the application of nitrogen fertilizers and effectively utilize mycorrhizal inoculation to improve grain yield and seed quality. These findings have implications for sustainable agriculture practices and guide farmers and researchers in making informed decisions regarding soil management strategies in fava bean cultivation.
Which EU and what for?
Authors: Scaruffi Roberto
Published: September 2023

The EU enlargement is inside the historical tendency of continental Europe to be dominated by a German-Russian axis. The “creation of Europe” has been the German continuation of WW1 and WW2 in other ways. Will the German tradition of State-building by custom unification lead to a simple Great Germany or instead to some kind of new State-formation, an original form of a new feudal State? Will this new space be the pure enlargement of the existing EU or should a greater EU inevitably lose western pieces? No easy institutional solutions exist for what is presently happening, while modernization of the EU States and of the EU as a whole is on the agenda for fully valorizing the chances of the unique market space.
For whom is realism?
Authors: Grätsch Marcus
Published: September 2023

In this paper I examine the question for whom realist theories are. Theories allow us to explain and understand the world and give the possibility to guide humans through actions. In the paper I elaborate this problem first. I than provide the reader with a short overview of realist theory in general and of Hans J. Morgenthau’s approach especially. To understand a possible purpose of Morgenthau I examine the social and political context in which Morgenthau developed his ideas. The following chapter than elaborates the usage of Morgenthau’s concept by some members of the scientific community. I contrast it with Robert Cox’s and Gramsci’s claim of hegemony.
Kosovo conflict
Authors: Iutes-Petrescu Alexandru , Carabu Gheorghe-Gabriel
Published: September 2023

The hypothesis: in the case of the Kosovo conflict, the international actors and organisations used new approach regarding intrastate conflicts. Because the international context is changing, our ways of dealing with intra-state conflicts try to adapt. With the help of some basic concepts, like humanitarian intervention, conflict transformation and new style of intervention, the paper analyses the international intervention and the projects that were thought for to help in configurating a long time solution for the crisis. The analysis focuses not only on NATO intervention, but also on EU reaction through its projects proposals for the region. The major core of a new approach is the idea of the responsibility of international organisations to protect, and the idea of solving conflicts through transformation.
Gone With the Wind
Authors: Ejalu William
Published: September 2023

The United Nations is continuously facing old and new challenges since the collapse of the Soviet Empire and the coming of the new world order. Nothing is more controversial in this Post-Communist Era than the questionable right of States and the International Community to intervene in so called ‘internal affairs’ of other States in order to protect the rights and lives of persons in conflict areas.
Refugees
Authors: Polese Abel
Published: September 2023

This article shows the attitude of the European Union towards refugees in the decennium 1990-1999, when the Balkan conflict broke out. It aims at demonstrating that, although it was clear that EU structures and legal framework were insufficient to deal with such a huge number of refugees, no satisfactory solution was found or proposed and negligence was the only reason behind ineffective international protection for people fleeing from the region. Part one spells out the legal framework, the definition of refugee and the progresses of the last 50 years in matter of international protection. Part two deals with the unclear statistics on refugees of these years, proposes an interpretation and shows some significant anomalies and part three describes the best mechanism (still too weak and adopted too late) provided by the EU in matter of refugees to face the massive income of refugees.
The “Smart City” between Urban Narrative and Empty Signifier: Shaggar in Focus
Authors: (PhD Candidate in Peace and Development Studies) Megersa Tolera
Published: September 2023

The objective of this study is to critically analyze the concept of the “Smart City” and its implications within the context of Shaggar. The study aims to explore the relationship between urban narratives and the empty signifiers associated with the “Smart City” concept, with a focus on understanding the underlying tensions and contradictions that arise during the implementation of smart urban initiatives. This study employs a qualitative research approach, utilizing a combination of document analysis, interviews, and observation techniques. Primary and secondary data sources, including official city plans, policy documents, media reports, and expert interviews, are used to gather information and insights. Thematic analysis is employed to identify recurring patterns and themes related to the “Smart City” concept, urban narratives, and empty signifiers in Shaggar. The findings of this study reveal that the “Smart City” concept in Shaggar is shaped by multiple urban narratives, including the discourse of technological progress, sustainability, and efficiency. However, the analysis also highlights the presence of empty signifiers, where the “Smart City” concept often lacks clear definitions, goals, and tangible outcomes. This disconnects between narratives and signifiers create tensions and challenges during the implementation of smart urban initiatives in Shaggar. In conclusion, this study demonstrates that the “Smart City” concept in Shaggar is a complex amalgamation of urban narratives and empty signifiers. The presence of multiple narratives contributes to the allure and promise of the “Smart City” vision, while the lack of clear signifiers results in ambiguity and challenges during implementation. This study underscores the need for critical examination and contextual understanding when envisioning and implementing “Smart City” initiatives. Based on the findings, this study recommends that policymakers, urban planners, and stakeholders in Shaggar and similar contexts should prioritize the development of clear and context-specific definitions, goals, and metrics for “Smart City” initiatives. It is crucial to engage with local communities, incorporate their perspectives, and ensure transparency and accountability in the implementation process. Furthermore, interdisciplinary collaborations and continuous evaluation of projects are essential to address the tensions between narratives and signifiers and to promote more inclusive and sustainable smart urban development in Shaggar and beyond.
Laudato Si'
Authors: Ramírez Valencia José Raúl , Fernández Luis Fernando
Published: September 2023

The five existential questions posed by Pope Francis in Laudato si' are addressed.  In a philosophical and theological fashion, he goes deeper into the importance of intergenerational responsibility. To answer these questions, the concepts of house, sister, mother, and common are analyzed in relation to ecology. Emphasis is put on the human being submerged in an individualistic and utilitarian society, which wonders more about the pragmatic and useful than the existential and, when this occurs, both society and the planet are affected. The final reflection poses the importance of anthropology which ponders and is responsible for generations living harmoniously with the common house.
Environmentally Responsible Bioengineering for Spore Surface Expression of Helicobacter pylori Antigen
Authors: Nguyen Thi Kim Cuc , Pham Thi Lan , Nguyen Thi Chinh , Pham Thu Thuy , Nguyen Van Duy
Published: September 2023

The development of genetic technologies and bioengineering are creating an increasing number of genetically engineered microorganisms with new traits for diverse industrial applications such as vaccines, drugs and pollutant degraders. However, the destiny of genetically engineered bacterial spores released into the environment as long-life organisms has remained a big environmental challenge. In this study, an environmentally responsible and sustainable gene technology solution based on the concept of thymine starvation is successfully applied for cloning and expression of a Helicobacter pylori antigen on Bacillus subtilis spore surface. As an example, a recombinant Bacillus subtilis strain A1.13 has been created from a gene fusion of the corresponding N-terminal fragment of spore coat protein CotB in B. subtilis and the entire urease subunit A (UreA) in H. pylori and the fusion showed a high stability of spore surface expression. The outcomes can open the door for developing highly safe spore vectored vaccines against this kind of pathogen and contributing to reduced potential risks of genetically engineered microorganisms released in the environment.
New Record: Molecular Depiction of Rhamnolipids (rhlA) Gene in Locally Isolated Strains of Pseudomonas aeruginosa
Authors: Al-Kanany Fadhil N. , Mohsen Najim
Published: September 2023

Soil samples were collected from oil-contaminated sites which were located in west Qurna, Basrah, Iraq. Pseudomonas species were initially isolated on mineral salts and Pseudomonas agar media and identified using morphological and biochemical characterizations. Then, specific primers for the rhlA gene belonging to Pseudomonas aeruginosa were designed based on the primer design conditions, and PCR was performed to amplify the 888 bp size fragment of the rhlA gene; additionally, the primary PCR products were purified and sent for sequencing. The band of about 888bp was determined on the gel, the amplified rhlA gene sequencing findings were revised, only 366 bp were ready to analyze using the (BLAST) software, and the final result was identified as a partial sequence of chromosomal rhlA gene related to Pseudomonas aeruginosa with percent identity of 99.45%. The query gene’s incomplete matching with another partial rhlA record on NCBI was caused by variations in two base pair sequences (T in sequence 348 and C in sequence 353, respectively), and despite the small difference, this results in variation in the amino acids produced; so that a new record number, ON637169, was assigned when the sequence was deposited in GenBank. The relation among the new record of partial rhlA gene with the same number of the other rhlA gene sequences (60 records) was demonstrated by creating a phylogenetic tree.
Evaluation of an In-house Indirect ELISA for Differential Detection of IgM and IgG anti-Brucella Antibodies in Human Brucellosis
Authors: Patra Sudipta , Sahib Muneera M. , Shanmugam G. , Skariah Somy , Shamshad Samer , Mohandoss Nagalingam , Shome Bibek R. , Shome Rajeswari
Published: September 2023

Brucellosis caused by various species of the genus Brucella is one of the most important zoonotic diseases of global importance with veterinary, public health, and economic concerns. The study aimed to standardize IgM and IgG-based iELISA to detect anti-Brucella antibodies for serodiagnosis of acute and chronic human brucellosis. The test was standardized using 1:320 dilution of smooth lipopolysaccharide (sLPS) antigen from B. abortus S99 strain, 1:80 serum dilution, 1:4000 anti-human IgM and IgG conjugates, respectively for both IgM and IgG iELISA. The cut-off using 50 each brucellosis positive and negative human sera panel samples was set at ≥ 42 for both IgM and IgG iELISA. A total of 700 human sera samples were evaluated (137 veterinary doctors, 157 artificial inseminators, and 406 veterinary assistants). Overall, the study detected 8.3%, 8.1%, 8%, and 6.1% positivity by in-house IgG iELISA, RBPT, IgM iELISA, and SAT tests, respectively. Considering commercial iELISA kit as a gold standard, the sensitivities of IgM and IgG iELISA were 90% and 97.9%, respectively, whereas, specificities were >99%. The study established >98% specificity and >90% sensitivity for differential detection of immunoglobulin classes in the standardized iELISA. The developed assay outperformed the other evaluated tests with a shorter assay time and can be implemented in both endemic and non-endemic regions for surveillance and diagnosis of human brucellosis.
Genomic Determinants and Antimicrobial Resistance Pattern of Clinical Isolates of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli
Authors: Shah Hamid Ahmad , Syed Arshi , Bhat Mohd Altaf , Kakru Dalip K. , Farooq Shaheen , Qureshi Sabia , Shafi Azhar , Nabi Burhan , Taku Anil
Published: September 2023

The growing prevalence of antibiotic-resistant bacteria is a worldwide public health apprehension, and Escherichia coli (E. coli) is one of the most commonly implicated bacterial species. Among E. coli isolates, extended-spectrum β-lactamase (ESBL)-producing strains have been identified as a key contributor to antibiotic resistance. Penicillin’s, cephalosporins, and monobactams are only a few of the β-lactam antibiotics that can be rendered inactive by ESBLs. This investigation’s goals were to determine the prevalence of ESBL-producing E. coli isolates found in clinical samples and to analyze the distribution of the blaTEM, blaSHV, and blaCTX-M genes among them. Additionally, we aimed to determine the antibiotic susceptibility patterns of these isolates to other antibiotics. Clinical isolates from urine, ear swab, and wound/pus swabs were collected from patients with suspected E. coli bacterial infections from different regions of north India viz., SKIMS-JVC medical college and NABL accredited Dr. Qadri’s Lab both from Srinagar Kashmir valley region and SMSR, SU from Greater Noida UP. Standard laboratory techniques were used to identify E. coli isolates, and the combined disc method and other phenotypic confirmation techniques were used to confirm ESBL formation. PCR amplification and sequencing were used to find the blaTEM, blaSHV, and blaCTX-M genes. The Kirby-Bauer disc diffusion method was used to test the antimicrobial susceptibility of various bacteria to different antibiotics. A total of 210 E. coli isolates were collected from different clinical samples and only 158 isolates showed positive results for ESBL by DDST and phenotypic confirmatory tests. Of these, 124 (78.48%) were ESBL-producing isolates. We found that blaTEM was the most prevalent gene (45.16%), followed by blaCTX-M (34.16%) and blaSHV (12.09%). Antimicrobial resistance profiles were assessed for each of the 120 isolates. Ampicillin and Cefepime were the most resistant drugs to ESBL-producing isolates, followed by Gentamicin, Ceftriaxone, and Cefixime.
Person Re-Identification Using Machine Learning
Authors: Dange Yogesh , Jadhar Pratik , Shirke Kshitija , Sangle Harshal , Navale Prof. Girish
Published: September 2023

Abstract: The goal of this research is to develop a system that can spot real-world anomalies in surveillance footage, like burglaries and assaults. Although different methods for anomaly detection have been explored in prior research, the significance of locality within the frame has not been fully explored. Given the rise in crime rates in many nations, accurate identification of people is essential for deterring and solving crimes. However, storing and processing information about suspects using conventional paper-based systems can be labor-intensive and time- consuming. This illustrates the need for more effective techniques for identifying dead bodies after a crime, which can be done using machine learning and image processing methods. Instead of using whole-frame video segments, this study explores the use of spatiotemporal tubes and presents a novel dataset for crime scene detection with bounding box supervision in both the train and test sets. The experiments show that a network trained with spatiotemporal tubes outperforms a model trained with whole-frame videos, with the locality remaining reliable even when extraction errors occur. Additionally, the network can expand the spatiotemporal crime scene dataset without the need for additional human labelling by producing spatiotemporal proposals for fresh surveillance videos using only video-level labels.
Preserving Privacy and Security: A Comparative Study of Health Data Regulations - GDPR vs. HIPAA
Authors: Shah Wasim Fathima
Published: September 2023

Abstract: The digital revolution has irreversibly transformed the healthcare landscape, revolutionizing the way health data is managed and raising critical questions about its protection. The seamless integration of technology has propelled healthcare into a new era of interconnectedness and data-driven innovation, presenting both unprecedented opportunities and challenges. In this context, this paper conducts a comprehensive comparative study of two pivotal regulatory frameworks: the General Data Protection Regulation (GDPR) and the Health Insurance Portability and Accountability Act (HIPAA). These regulations, enacted on different continents, serve as cornerstones in the protection of health data and offer profound insights into the evolving landscape of data privacy and security in the digital age.The surge in digital health platforms, electronic medical records, and data-sharing networks has ushered in an era where personal health information is more vulnerable and valuable than ever before. The GDPR, a landmark legislation within the European Union, empowers individuals with control over their personal data and sets stringent standards for data protection. On the other side of the Atlantic, HIPAA plays a fundamental role in regulating health data in the United States, emphasizing the confidentiality, integrity, and availability of electronic health information.This study aims to unravel the intricate tapestry of health data protection by juxtaposing the GDPR and HIPAA. By exploring their commonalities and disparities, this analysis offers insights into the multifaceted dimensions of safeguarding health data in an interconnected world. Delving into the legal principles, individual rights, organizational responsibilities, security prerequisites, procedural intricacies, and enforcement mechanisms embedded within these frameworks, this study seeks to inform stakeholders and decision-makers about the critical nuances of health data governance.Employing a comprehensive research approach that draws from primary legal sources, scholarly interpretations, case studies, and comparative analyses, this study enriches our understanding of the intricate interplay between regulation and technology. By shedding light on the regulatory strategies of the GDPR and HIPAA, stakeholders can better navigate the intricate terrain of health data protection and contribute to the development of a privacy-respecting and technologically vibrant healthcare ecosystem.
Points de vue 2STNBNCG sur l’accès à la justice: Le zine
Authors:   JusticeTrans,   Carleton University Research Ethics Board-A (CUREB-A),   Femmes et Égalité des genres Canada
Published: September 2023

Nous avons vu de nombreux changements positifs à la loi au Canada au cours de la décennie précédente pour mieux répondre aux besoins de personnes bispirituelles, trans, non binaires et non conformes au genre (2STNBNCG) – dont l’inclusion de l’identité et/ou l’expression de genre dans la législation sur les droits de la personne et la possibilité de changer son nom et son identifiant de genre sur les documents gouvernementaux sans devoir subir une intervention chirurgicale d’affirmation de genre. Nous avons également vu une augmentation de l’extrémisme au Canada, aux États-Unis et au Royaume-Uni, menant à une augmentation remarquable de haine anti-trans. Nos propres expériences et les résultats d’études de recherche montrent que les personnes 2STNBNCG subissent encore beaucoup de violence et d’injustice. Il est important de comprendre les besoins de communautés 2STNBNCG de tout le Canada pour mieux promouvoir la libération et la justice 2STNBNCG et pour contribuer à la lutte contre la haine anti-tra
Autorías que dentro de 10 años inspirarán el pensamiento latinoamericano. Ejercicio de prognosis
Authors: Devés Valdés Eduardo
Published: September 2023

Se estudian las citaciones de 30 a 35 autorías de prosa de ideas originarias de la región, intentando hipotetizar sobre lo que ocurrirá con estas dentro de 10 a 12 años. El objetivo de largo plazo consiste en presentar un tipo de trabajo de prognosis en el estudio de las ideas, para lo cual se realiza una investigación que cuenta con 3 objetivos específicos. El primero consiste en determinar el nivel relativo de citaciones de algunas de las figuras más importantes de la prosa de ideas que han marcado líneas del pensamiento latinoamericano a finales del siglo 20 y sobre todo en las 2 primeras décadas del 21. El segundo, presentar un conjunto de criterios que permitirían fundar las hipótesis de prognosis. El tercero, consiste en formular un pronóstico fundado sobre el nivel de citaciones de estas autorías, dentro de una década y un poco más, entre 2030 y 2035.Para este caso de prognosis se combinan 3 metodologías: la consulta a personas expertas para determinar las figuras de mayores citaciones, a mis trabajos anteriores y al empleo de la información existente en internet entre 2000 y 2020, para proyectar lo que ocurrirá hacia los años 2032-2035. Se combinan técnicas cuantitativas, como cifras y relaciones entre estas, con interpretaciones cualitativas y teóricas que permiten darles mayor sentido.Estas prognosis pueden tener alta significación para conocer las tendencias eidéticas que estarían en juego dentro de 10 a 15 años, en los ecosistemas intelectivos de América Latina Caribe.
Analisis Kemampuan Guru SMP dalam Mengintegrasikan Nilai-Nilai Islami pada Pembelajaran Seni (Studi Penelitian di MTsS Darul Ulum Banda Aceh dan SMP IT Al-Fityan Aceh Besar)
Authors: Zulkarnaen Zulkarnaen
Published: September 2023

Art learning as one of the sciences taught in schools must be able to answer the challenge that Islamic values education can be taught through learning in class. The fact shows that art learning which has been carried out in schools is considered less effective. The purpose of this study was to determine the teacher's ability, the application process and the supporting and inhibiting factors in integrating Islamic values in art learning. This research use desciptive qualitative approach. Data obtained by interview, observation and documentation. The research informants were school principals, curriculum representatives, art teachers, and students at MTsS Darul Ulum Banda Aceh and SMPIT Al-Fityan Aceh Besar. The results of the study show the teacher's ability to integrate Islamic values in art learning: (a) MTsS Darul Ulum Banda Aceh; Teachers find it difficult to find Islamic values that are exactly equivalent to material concepts. (b) SMPIT Al-Fityan Aceh Besar; The teacher is able to relate Islamic values by following the hadith references narrated by Bukhari and Muslim. The implementation process carried out: (a) MTsS Darul Ulum Banda Aceh; at the discussion stage the teacher tries to associate it with Islamic values. (b) SMPIT Al-Fityan Aceh Besar; The teacher tries to explain points about the afterlife messages as well as Islamic teachings. Supporting factors include the educational environment that combines general education and Islamic boarding school education, while the inhibiting factors are the teacher's ability level, which still has difficulties in integrating material.
Development of RP-HPLC Method for Simultaneous Determination of Bilastine and Montelukast by Qbd Approach and Its Validation
Authors: Ahmed Aejaz , Mehfuza U Manjra , Mubina Lajporiya , Nazifa Sayyed , Seema Patel , Khan G.J. , Majaz Ahamad Qazi
Published: September 2023

This study proposes to develop and validate the RP-HPLC method for Bilastine (BIL) and Montelukast (MKT) byQbD to substantiate the RP-HPLC analysis as per ICH validation guidelines. Quality by Design (QbD) allows the accomplishmentof specific unsurprising quality with a predetermined and wanted determination. The simultaneous estimation of BIL and MKTwas performed with C18 (4.6×250 mm, 5-μm particle size) with an LC-10AD pump and PDA detector. The mobile phaseemployed methanol and ammonium acetate buffer pH-3.6 at 85:15 v/v. The flow rate was maintained at 1.0 ml/min, and BIL andMKT were detected at 249nm and 293 nm by UV detector, respectively. The HPLC method provided linear responses found inthe 200–600 μg/ml range. The correlation coefficient was 0.9995 for BIL and 0.9991 for MKT. The LOD and LOQ for BIL andMKT were found to be 0.493, 1.495 μg/ml, and 0.693, 2.100, respectively. The percentage recovery for BIL was 95.33 to 102.06,and for MKT was 96.31 to 104.05, respectively. Calculated information acquired for both the preliminaries roughly coordinateswith the information given by Design expert programming, showing the chromatographic condition's genuineness. Design-Expertversion 10 ("DX10") software has calculated this calculation, setting a composite design of significant parameters. A newselective, rapid, accurate, precise, and sensitive RP-HPLC method was developed and evaluated for the simultaneousdetermination of Bilastine (BIL) and Montelukast sodium (MKT) in a bulk and pharmaceutical dosage form. This method is usefulin the routine quality analysis of combinations of BIL and MKT in bulk and its tablet formulations.
Práticas de inovação por meio da indústria criativa: um estudo na região do Paranhana/RS
Authors: Zilles Borba Eduardo , Portal Valmir Mateus , Rodrigues Marley
Published: September 2023

O artigo discute práticas de inovação originadas em ações de organizações vinculadas à indústria criativa, especificamente na região do Paranhana (RS). Parte-se do princípio de que a indústria criativa possui potencial para colaborar com o desenvolvimento regional, valorizando práticas de produção e consumo intrínsecas ao capital intelectual e cultural de determinados territórios. Lança-se o seguinte problema: de que forma organizações na região do Paranhana se apropriamdas características da indústria criativa para estimular a inovação? Por meio de metodologia exploratória e descritiva, foram conduzidas entrevistas com gestores, a fim de coletar e analisar dados qualitativos sobre suas experimentações criativas que culminam em ações inovadoras. Como resultado é apresentado levantamento de práticas de inovação impulsionadas pelas indústrias criativas na região, sendo identificada tendência para a ação de empresas ou consultorias criativas ligadas mais à otimização de processos de organizações tradicionais do que propriamente à criação deprodutos/serviços. Em suma, os resultados surgem como mapeamento inicial de práticas de inovação por meio da indústria criativa, revelando-se instrumento útil para a ação de organizações nessa região.