bluuebunny/crossref_metadata_2025 huggingface dataset:
A GIS-Based Evacuation Route Planning in Flood-Susceptible Area of Siraha Municipality, Nepal
Authors: Parajuli Gaurav , Neupane Shankar , Kunwar Sandeep , Adhikari Ramesh , Acharya Tri Dev
Published: July 2023

Flood is one of the most frequently occurring and devastating disasters in Nepal. Several locations in Nepal are at high risk of flood, which requires proper guidance on early warning and safe evacuation of people to emergency locations through optimal routes to minimize fatalities. However, the information is limited to flood hazard mapping only. This study provides a comprehensive flood susceptibility and evacuation route mapping in the Siraha Municipality of Nepal where a lot of flood events have occurred in the past and are liable to happen in the future. The flood susceptibility map was created using a Geographic Information System (GIS)-based Analytical Hierarchy Process (AHP) over nine flood conditioning factors. It showed that 47% of the total area was highly susceptible to flood, and the remaining was in the safe zone. The assembly points where people would gather for evacuation were selected within the susceptible zone through manual digitization while the emergency shelters were selected within a safe zone such that they can host the maximum number of people. The network analysis approach is used for evacuation route mapping in which the closest facility analysis proposed the optimum evacuation route based on the walking speed of evacuees to reach the emergency shelter place considering the effect of slope and flood on the speed of the pedestrian. A total of 12 out of 22 suggested emergency shelters were within 30 min, 7 within 60 min, and 2 within 100 min walk from the assembly point. Moreover, this study suggests the possible areas for further shelter place allocations based on service area analysis. This study can support the authorities’ decision-making for the flood risk assessment and early warning system planning, and helps in providing an efficient evacuation plan for risk mitigation.
Gut Microbiome and Microbiome-Derived Metabolites in Patients with End-Stage Kidney Disease
Authors: Koshida Takeo , Gohda Tomohito , Sugimoto Takuya , Asahara Takashi , Asao Rin , Ohsawa Isao , Gotoh Hiromichi , Murakoshi Maki , Suzuki Yusuke , Yamashiro Yuichiro
Published: July 2023

The composition of the gut microbiome is altered in patients with chronic kidney disease (CKD). Dysbiosis leads to decreased levels of stool organic acids (OAs) and systemic inflammation, followed by accumulation of uremic toxins (UTs) and the development of end-stage kidney disease (ESKD). We assessed the relationship between the microbiome and UT levels or the development of ESKD by comparing patients undergoing hemodialysis (HD) and those with normal renal function (NRF). This cross-sectional study recruited 41 patients undergoing HD and 38 sex- and age-matched patients with NRF, and gut microbiome, levels of plasma UTs, inflammatory markers, and stool OAs were compared. The indices of beta-diversity differed significantly between patients with NRF and those undergoing HD, and between patients undergoing HD with and without type 2 diabetes. The levels of stool total OA, inflammatory markers, and UTs differed significantly between the patients with NRF and those undergoing HD. The combined main effects of type 2 diabetes and kidney function status were accumulation of indoxyl sulfate and p-cresyl sulfate. The relative abundances of Negativicutes and Megamonas were associated with development of ESKD and with the levels of UTs, even after adjustment for factors associated with the progression of ESKD. The present study indicates that the gut environment differs between patients with NRF and those undergoing HD and between patients undergoing HD with and without type 2 diabetes. Moreover, ESKD patients with diabetes accumulate more UTs derived from the gut microbiome, which might be associated with cardio-renal diseases and poor prognosis.
Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses
Authors: Baek Dongwon , Cho Hyun Min , Cha Ye Jin , Jin Byung Jun , Lee Su Hyeon , Park Mi Suk , Chun Hyun Jin , Kim Min Chul
Published: July 2023

The calmodulin-binding transcription activators (CAMTAs) mediate transcriptional regulation of development, growth, and responses to various environmental stresses in plants. To understand the biological roles of soybean CAMTA (GmCAMTA) family members in response to abiotic stresses, we characterized expression patterns of 15 GmCAMTA genes in response to various abiotic stresses. The GmCAMTA genes exhibited distinct circadian regulation expression patterns and were differently expressed in response to salt, drought, and cold stresses. Interestingly, the expression levels of GmCAMTA2, GmCAMTA8, and GmCAMTA12 were higher in stem tissue than in other soybean tissues. To determine the roles of GmCAMTAs in the regulation of developmental processes and stress responses, we isolated GmCAMTA2 and GmCAMTA8 cDNAs from soybean and generated Arabidopsis overexpressing transgenic plants. The GmCAMTA2-OX and GmCAMTA8-OX plants showed hypersensitivity to drought stress. The water in the leaves of GmCAMTA2-OX and GmCAMTA8-OX plants was lost faster than that in wild-type (WT) plants under drought-stress conditions. In addition, stress-responsive genes were down-regulated in the GmCAMTA2-OX and GmCAMTA8-OX plants under drought stress conditions compared to WT plants. Our results suggest that GmCAMTA2 and GmCAMTA8 genes are regulated by circadian rhythms and function as negative regulators in development and drought stress responses.
Role of Mouse Organic Cation Transporter 2 for Nephro- and Peripheral Neurotoxicity Induced by Chemotherapeutic Treatment with Cisplatin
Authors: Hucke Anna , Schröter Rita , Ceresa Cecilia , Chiorazzi Alessia , Canta Annalisa , Semperboni Sara , Marmiroli Paola , Cavaletti Guido , Gess Burkhard , Ciarimboli Giuliano
Published: July 2023

Cisplatin (CDDP) is an efficient chemotherapeutic agent broadly used to treat solid cancers. Chemotherapy with CDDP can cause significant unwanted side effects such as renal toxicity and peripheral neurotoxicity. CDDP is a substrate of organic cation transporters (OCT), transporters that are highly expressed in renal tissue. Therefore, CDDP uptake by OCT may play a role in causing unwanted toxicities of CDDP anticancer treatment. In this study, the contribution of the mouse OCT2 (mOCT2) to CDDP nephro- and peripheral neurotoxicity was investigated by comparing the effects of cyclic treatment with low doses of CDDP on renal and neurological functions in wild-type (WT) mice and mice with genetic deletion of OCT2 (OCT2−/− mice). This CDDP treatment protocol caused significant impairment of kidneys and peripherical neurological functions in WT mice. These effects were significantly reduced in OCT2−/− mice, however, less profoundly than what was previously measured in mice with genetic deletion of both OCT1 and 2 (OCT1-2−/− mice). Comparing the apparent affinities (IC50) of mOCT1 and mOCT2 for CDDP, the mOCT1 displayed a higher affinity for CDDP than the mOCT2 (IC50: 9 and 558 µM, respectively). Also, cellular toxicity induced by incubation with 100 µM CDDP was more pronounced in cells stably expressing mOCT1 than in cells expressing mOCT2. Therefore, in mice, CDDP uptake by both OCT1 and 2 contributes to the development of CDDP undesired side effects. OCT seem to be suitable targets for establishing treatment protocols aimed at decreasing unwanted CDDP toxicity and improving anticancer treatment with CDDP.
Carbamoylated Erythropoietin-Induced Cerebral Blood Perfusion and Vascular Gene Regulation
Authors: Sadanandan Jayanarayanan , Sathyanesan Monica , Liu Yutong , Tiwari Neeraj K. , Newton Samuel S.
Published: July 2023

Cerebral hypoperfusion is associated with enhanced cognitive decline and increased risk of neuropsychiatric disorders. Erythropoietin (EPO) is a neurotrophic factor known to improve cognitive function in preclinical and clinical studies of neurodegenerative and psychiatric disorders. However, the clinical application of EPO is limited due to its erythropoietic activity that can adversely elevate hematocrit in non-anemic populations. Carbamoylated erythropoietin (CEPO), a chemically engineered non-erythropoietic derivative of EPO, does not alter hematocrit and maintains neurotrophic and behavioral effects comparable to EPO. Our study aimed to investigate the role of CEPO in cerebral hemodynamics. Magnetic resonance imaging (MRI) analysis indicated increased blood perfusion in the hippocampal and striatal region without altering tight junction integrity. In vitro and in vivo analyses indicated that hippocampal neurotransmission was unaltered and increased cerebral perfusion was likely due to EDRF, CGRP, and NOS-mediated vasodilation. In vitro analysis using human umbilical vein endothelial cells (HUVEC) and hippocampal vascular gene expression analysis showed CEPO to be a non-angiogenic agent which regulates the MEOX2 gene expression. The results from our study demonstrate a novel role of CEPO in modulating cerebral vasodilation and blood perfusion.
Antioxidant Defense Capacity Is Reduced in Thyroid Stem/Precursor Cells Compared to Differentiated Thyrocytes
Authors: Gianì Fiorenza , Allia Fabio , Trovato Maria Antonietta , Masto Roberta , Pellegriti Gabriella , Vigneri Riccardo
Published: July 2023

There is much evidence linking oxidative stress to thyroid cancer, and stem cells are thought to play a key role in the tumor-initiating mechanism. Their vulnerability to oxidative stress is unexplored. This study aimed to comparatively evaluate the antioxidant capacity of stem/precursor thyroid cells and mature thyrocytes. Human stem/precursor cells and mature thyrocytes were exposed to increasing concentrations of menadione, an oxidative-stress-producing agent, and reactive oxygen species (ROS) production and cell viability were measured. The expression of antioxidant and detoxification genes was measured via qPCR as well as the total antioxidant capacity and the content of glutathione. Menadione elevated ROS generation in stem/precursor thyroid cells more than in mature thyrocytes. The ROS increase was inversely correlated (p = 0.005) with cell viability, an effect that was partially prevented by the antioxidant curcumin. Most thyroid antioxidant defense genes, notably those encoding for the glutathione-generating system and phase I detoxification enzymes, were significantly less expressed in stem/precursor thyroid cells. As a result, the glutathione level and the total antioxidant capacity in stem/precursor thyroid cells were significantly decreased. This reduced antioxidant defense may have clinical implications, making stem/precursor thyroid cells critical targets for environmental conditions that are not detrimental for differentiated thyrocytes.
Sex Differences in the Frequencies of B and T Cell Subpopulations of Human Cord Blood
Authors: Bous Michelle , Schmitt Charline , Hans Muriel Charlotte , Weber Regine , Nourkami-Tutdibi Nasenien , Tenbruck Sebastian , Haj Hamoud Bashar , Wagenpfeil Gudrun , Kaiser Elisabeth , Solomayer Erich-Franz , Zemlin Michael , Goedicke-Fritz Sybelle
Published: July 2023

Cord blood represents a link between intrauterine and early extrauterine development. Cord blood cells map an important time frame in human immune imprinting processes. It is unknown whether the sex of the newborn affects the lymphocyte subpopulations in the cord blood. Nine B and twenty-one T cell subpopulations were characterized using flow cytometry in human cord blood from sixteen male and twenty-one female newborns, respectively. Except for transitional B cells and naïve B cells, frequencies of B cell counts across all subsets was higher in the cord blood of male newborns than in female newborns. The frequency of naïve thymus-negative Th cells was significantly higher in male cord blood, whereas the remaining T cell subpopulations showed a higher count in the cord blood of female newborns. Our study is the first revealing sex differences in the B and T cell subpopulations of human cord blood. These results indicate that sex might have a higher impact for the developing immune system, urging the need to expand research in this area.
A New Strategy for the Old Challenge of Thalidomide: Systems Biology Prioritization of Potential Immunomodulatory Drug (IMiD)-Targeted Transcription Factors
Authors: Kowalski Thayne Woycinck , Feira Mariléa Furtado , Lord Vinícius Oliveira , Gomes Julia do Amaral , Giudicelli Giovanna Câmara , Fraga Lucas Rosa , Sanseverino Maria Teresa Vieira , Recamonde-Mendoza Mariana , Schuler-Faccini Lavinia , Vianna Fernanda Sales Luiz
Published: July 2023

Several molecular mechanisms of thalidomide embryopathy (TE) have been investigated, from anti-angiogenesis to oxidative stress to cereblon binding. Recently, it was discovered that thalidomide and its analogs, named immunomodulatory drugs (IMiDs), induced the degradation of C2H2 transcription factors (TFs). This mechanism might impact the strict transcriptional regulation of the developing embryo. Hence, this study aims to evaluate the TFs altered by IMiDs, prioritizing the ones associated with embryogenesis through transcriptome and systems biology-allied analyses. This study comprises only the experimental data accessed through bioinformatics databases. First, proteins and genes reported in the literature as altered/affected by the IMiDs were annotated. A protein systems biology network was evaluated. TFs beta-catenin (CTNNB1) and SP1 play more central roles: beta-catenin is an essential protein in the network, while SP1 is a putative C2H2 candidate for IMiD-induced degradation. Separately, the differential expressions of the annotated genes were analyzed through 23 publicly available transcriptomes, presenting 8624 differentially expressed genes (2947 in two or more datasets). Seventeen C2H2 TFs were identified as related to embryonic development but not studied for IMiD exposure; these TFs are potential IMiDs degradation neosubstrates. This is the first study to suggest an integration of IMiD molecular mechanisms through C2H2 TF degradation.
Nitazoxanide Inhibits the Bifunctional Enzyme GlG6PD::6PGL of Giardia lamblia: Biochemical and In Silico Characterization of a New Druggable Target
Authors: Martínez-Rosas Víctor , Hernández-Ochoa Beatriz , Morales-Luna Laura , Ortega-Cuellar Daniel , González-Valdez Abigail , Arreguin-Espinosa Roberto , Rufino-González Yadira , Calderón-Jaimes Ernesto , Castillo-Rodríguez Rosa Angélica , Wong-Baeza Carlos , Baeza-Ramírez Isabel , Pérez de la Cruz Verónica , Vidal-Limón Abraham , Gómez-Manzo Saúl
Published: July 2023

Giardiasis, which is caused by Giardia lamblia infection, is a relevant cause of morbidity and mortality worldwide. Because no vaccines are currently available to treat giardiasis, chemotherapeutic drugs are the main options for controlling infection. Evidence has shown that the nitro drug nitazoxanide (NTZ) is a commonly prescribed treatment for giardiasis; however, the mechanisms underlying NTZ’s antigiardial activity are not well-understood. Herein, we identified the glucose-6-phosphate::6-phosphogluconate dehydrogenase (GlG6PD::6PGL) fused enzyme as a nitazoxanide target, as NTZ behaves as a GlG6PD::6PGL catalytic inhibitor. Furthermore, fluorescence assays suggest alterations in the stability of GlG6PD::6PGL protein, whereas the results indicate a loss of catalytic activity due to conformational and folding changes. Molecular docking and dynamic simulation studies suggest a model of NTZ binding on the active site of the G6PD domain and near the structural NADP+ binding site. The findings of this study provide a novel mechanistic basis and strategy for the antigiardial activity of the NTZ drug.
Comparison between Cultivated Oral Mucosa and Ocular Surface Epithelia for COMET Patients Follow-Up
Authors: Attico Eustachio , Galaverni Giulia , Torello Andrea , Bianchi Elisa , Bonacorsi Susanna , Losi Lorena , Manfredini Rossella , Lambiase Alessandro , Rama Paolo , Pellegrini Graziella
Published: July 2023

Total bilateral Limbal Stem Cell Deficiency is a pathologic condition of the ocular surface due to the loss of corneal stem cells. Cultivated oral mucosa epithelial transplantation (COMET) is the only autologous successful treatment for this pathology in clinical application, although abnormal peripheric corneal vascularization often occurs. Properly characterizing the regenerated ocular surface is needed for a reliable follow-up. So far, the univocal identification of transplanted oral mucosa has been challenging. Previously proposed markers were shown to be co-expressed by different ocular surface epithelia in a homeostatic or perturbated environment. In this study, we compared the transcriptome profile of human oral mucosa, limbal and conjunctival cultured holoclones, identifying Paired Like Homeodomain 2 (PITX2) as a new marker that univocally distinguishes the transplanted oral tissue from the other epithelia. We validated PITX2 at RNA and protein levels to investigate 10-year follow-up corneal samples derived from a COMET-treated aniridic patient. Moreover, we found novel angiogenesis-related factors that were differentially expressed in the three epithelia and instrumental in explaining the neovascularization in COMET-treated patients. These results will support the follow-up analysis of patients transplanted with oral mucosa and provide new tools to understand the regeneration mechanism of transplanted corneas.
Vascular Ageing: Mechanisms, Risk Factors, and Treatment Strategies
Authors: Ya Jingyuan , Bayraktutan Ulvi
Published: July 2023

Ageing constitutes the biggest risk factor for poor health and adversely affects the integrity and function of all the cells, tissues, and organs in the human body. Vascular ageing, characterised by vascular stiffness, endothelial dysfunction, increased oxidative stress, chronic low-grade inflammation, and early-stage atherosclerosis, may trigger or exacerbate the development of age-related vascular diseases, which each year contribute to more than 3.8 million deaths in Europe alone and necessitate a better understanding of the mechanisms involved. To this end, a large number of recent preclinical and clinical studies have focused on the exponential accumulation of senescent cells in the vascular system and paid particular attention to the specific roles of senescence-associated secretory phenotype, proteostasis dysfunction, age-mediated modulation of certain microRNA (miRNAs), and the contribution of other major vascular risk factors, notably diabetes, hypertension, or smoking, to vascular ageing in the elderly. The data generated paved the way for the development of various senotherapeutic interventions, ranging from the application of synthetic or natural senolytics and senomorphics to attempt to modify lifestyle, control diet, and restrict calorie intake. However, specific guidelines, considering the severity and characteristics of vascular ageing, need to be established before widespread use of these agents. This review briefly discusses the molecular and cellular mechanisms of vascular ageing and summarises the efficacy of widely studied senotherapeutics in the context of vascular ageing.
Assessing the Solid Protocol in Relation to Security and Privacy Obligations
Authors: Esposito Christian , Horne Ross , Robaldo Livio , Buelens Bart , Goesaert Elfi
Published: July 2023

The Solid specification aims to empower data subjects by giving them direct access control over their data across multiple applications. As governments are manifesting their interest in this framework for citizen empowerment and e-government services, security and privacy represent pivotal issues to be addressed. By analysing the relevant legislation, with an emphasis on GDPR and officially approved documents such as codes of conduct and relevant security ISO standards, we formulate the primary security and privacy requirements for such a framework. The legislation places some obligations on pod providers, much like cloud services. However, what is more interesting is that Solid has the potential to support GDPR compliance of Solid apps and data users that connect, via the protocol, to Solid pods containing personal data. A Solid-based healthcare use case is illustrated where identifying such controllers responsible for apps and data users is essential for the system to be deployed. Furthermore, we survey the current Solid protocol specifications regarding how they cover the highlighted requirements, and draw attention to potential gaps between the specifications and requirements. We also point out the contribution of recent academic work presenting novel approaches to increase the security and privacy degree provided by the Solid project. This paper has a twofold contribution to improve user awareness of how Solid can help protect their data and to present possible future research lines on Solid security and privacy enhancements.
Arabic Mispronunciation Recognition System Using LSTM Network
Authors: Ahmed Abdelfatah , Bader Mohamed , Shahin Ismail , Nassif Ali Bou , Werghi Naoufel , Basel Mohammad
Published: July 2023

The Arabic language has always been an immense source of attraction to various people from different ethnicities by virtue of the significant linguistic legacy that it possesses. Consequently, a multitude of people from all over the world are yearning to learn it. However, people from different mother tongues and cultural backgrounds might experience some hardships regarding articulation due to the absence of some particular letters only available in the Arabic language, which could hinder the learning process. As a result, a speaker-independent and text-dependent efficient system that aims to detect articulation disorders was implemented. In the proposed system, we emphasize the prominence of “speech signal processing” in diagnosing Arabic mispronunciation using the Mel-frequency cepstral coefficients (MFCCs) as the optimum extracted features. In addition, long short-term memory (LSTM) was also utilized for the classification process. Furthermore, the analytical framework was incorporated with a gender recognition model to perform two-level classification. Our results show that the LSTM network significantly enhances mispronunciation detection along with gender recognition. The LSTM models attained an average accuracy of 81.52% in the proposed system, reflecting a high performance compared to previous mispronunciation detection systems.
Dependence of Ge/Si Avalanche Photodiode Performance on the Thickness and Doping Concentration of the Multiplication and Absorption Layers
Authors: Deeb Hazem , Khomyakova Kristina , Kokhanenko Andrey , Douhan Rahaf , Lozovoy Kirill
Published: July 2023

In this article, the performance and design considerations of the planar structure of germanium on silicon avalanche photodiodes are presented. The dependences of the breakdown voltage, gain, bandwidth, responsivity, and quantum efficiency on the reverse bias voltage for different doping concentrations and thicknesses of the absorption and multiplication layers of germanium on the silicon avalanche photodiode were simulated and analyzed. The study revealed that the gain of the avalanche photodiode is directly proportional to the thickness of the multiplication layer. However, a thicker multiplication layer was also associated with a higher breakdown voltage. The bandwidth of the device, on the other hand, was inversely proportional to the product of the absorption layer thickness and the carrier transit time. A thinner absorption layer offers a higher bandwidth, but it may compromise responsivity and quantum efficiency. In this study, the dependence of the photodetectors’ operating characteristics on the doping concentration used for the multiplication and absorption layers is revealed for the first time.
Culicoides (Diptera: Ceratopogonidae) Abundance Is Influenced by Livestock Host Species and Distance to Hosts at the Micro Landscape Scale
Authors: Elbers Armin R. W. , Gonzales José L.
Published: July 2023

The vector/host ratio and host preference are important parameters for the modelling of vector-borne livestock diseases. It can be anticipated that Culicoides abundance is not homogeneously distributed in the landscape. We investigated the influence of host species (dairy cow, sheep, and a light-trap (LT) as a surrogate host) and distance of measurement to hosts on Culicoides abundance using a randomized block-design with 12 measuring days and seven 3-min aerial sweep-netting sessions per whole hour at three distances to the host (0, 10, and 25 m), from five hours before to and including one hour after sunset. Dairy cows were found to be a far stronger attractor of Culicoides midges than sheep, while both hosts were far stronger attractors of midges than the LT. Culicoides abundance declined significantly with increasing distance from the livestock hosts; this phenomenon was much stronger for dairy cows than for ewes. In contrast, Culicoides abundance increased with increasing distance from the LT, pin-pointing the apparent shortcomings of the LT as a surrogate host to lure midges. Our data indicate that livestock host species and the distance from these hosts have a profound effect on Culicoides abundance in the landscape.
Influences of Microbial Symbionts on Chemoreception of Their Insect Hosts
Authors: Wang Zhengyan , Chang Zhenzhen , Liu Zhiyuan , Zhang Shan
Published: July 2023

Chemical communication is widespread among insects and exploited to adjust their behavior, such as food and habitat seeking and preferences, recruitment, defense, and mate attraction. Recently, many studies have revealed that microbial symbionts could regulate host chemical communication by affecting the synthesis and perception of insect semiochemicals. In this paper, we review recent studies of the influence of microbial symbionts on insect chemoreception. Microbial symbionts may influence insect sensitivity to semiochemicals by regulating the synthesis of odorant-binding proteins or chemosensory proteins and olfactory or gustatory receptors and regulating host neurotransmission, thereby adjusting insect behavior. The manipulation of insect chemosensory behavior by microbial symbionts is conducive to their proliferation and dispersal and provides the impetus for insects to change their feeding habits and aggregation and dispersal behavior, which contributes to population differentiation in insects. Future research is necessary to reveal the material and information exchange between both partners to improve our comprehension of the evolution of chemoreception in insects. Manipulating insect chemoreception physiology by inoculating them with microbes could be utilized as a potential approach to managing insect populations.
Effects of Temperature on the Developmental and Reproductive Biology of North American Bean Thrips, Caliothrips fasciatus (Pergande) (Thysanoptera: Thripidae: Panchaetothripinae)
Authors: Hoddle Mark S. , Milosavljević Ivan , Amrich Ruth
Published: July 2023

North American bean thrips, Caliothrips fasciatus, native to California U.S., has been detected inside the navels of navel oranges exported from California for more than 120 years. Despite this long history of accidental movement into new areas, this thrips has failed to establish populations outside of its native range. The cold accumulation hypothesis postulates that increasing levels of cold stress experienced by thrips overwintering inside navels is compounded when harvested fruit is shipped under cold storage conditions. Consequently, the fitness of surviving thrips is compromised, which greatly diminishes invasion potential. At the time this study was conducted, the effects of temperature on C. fasciatus fitness were unknown. To address this shortcoming, the effects of nine fluctuating temperatures that averaged 8, 10, 15, 20, 25, 30, 32, 35, and 37 °C over a 24 h period on the developmental and reproductive biology of C. fasciatus were evaluated. One linear and five nonlinear regression functions were fit to egg-to-adult development rate data for parent and offspring thrips to characterize thermal performance curves. Estimates of minimum, optimal, and maximum temperature thresholds for development were in the ranges of −4.37–6.52 °C (i.e., Tmin), 31.19–32.52 °C (i.e., Topt), and 35.07–37.98 °C (i.e., Tmax), respectively. Degree day accumulation to complete development, estimated from linear regression, ranged 370.37–384.61. Average development times for eggs, first and second instar larvae, propupae, pupae, and adult longevity, and mean lifetime fecundity of females were significantly affected by temperature. These biological responses to temperature may provide insight into how this abiotic variable affects the invasion potential of C. fasciatus.
First Discovery of the North American Leaf-Mining Moth Chrysaster ostensackenella (Lepidoptera: Gracillariidae) in Russia: The Genetic Diversity of a Novel Pest in Invaded vs. Native Range
Authors: Kirichenko Natalia I. , Kolyada Nina A. , Gomboc Stanislav
Published: July 2023

Here, we report the first detection of the North American leaf-mining moth Chrysaster ostensackenella (Fitch, 1859) (Lepidoptera: Gracillariidae) on North American black locust Robinia pseudoacacia (Fabaceae) in Primorsky Krai (the Russian Far East) in July 2022. Overall, six moths were reared from the leaf mines and identified based on adult morphology (forewing pattern and male genitalia) and three of them were DNA barcoding. Description of the leaf mines that allowed us to distinguish the damage of Ch. ostensackenella from other gracillariids associated with R. pseudoacacia is provided. The phylogeographic analysis comparing the DNA barcodes from Russia with those from other invaded countries in Europe (Italy) and East Asia (South Korea and Japan) and from the native range (North America) was performed. Intraspecific genetic diversity reached 3.29%. Altogether, 10 haplotypes were revealed among 21 studied specimens in the Holarctic. The detection of one haplotype common for Japan and the USA (North Carolina) suggests that the invasion to East Asia could have happened from the USA directly, rather than through Europe. A shared haplotype defined for Japan and the Russian Far East points at a possible moth species’ spread to Primorsky Krai from earlier invaded Hokkaido. Further distribution of Ch. ostensackenella in East Asia and Europe is expected, bearing in mind the wide planting of R. pseudoacacia in these continents. Furthermore, an accidental introduction of the moth to the Southern Hemisphere, where black locust was introduced, is not ruled out.
Mental Health of Transgender Youth: A Comparison of Assigned Female at Birth and Assigned Male at Birth Individuals
Authors: Klinger Diana , Riedl Stefan , Zesch Heidi Elisabeth , Oehlke Sofia-Marie , Völkl-Kernstock Sabine , Plener Paul L. , Karwautz Andreas , Kothgassner Oswald D.
Published: July 2023

Gender dysphoric adolescents report a gender identity which is incongruent with their assigned sex at birth, whereby the experienced incongruence is accompanied by clinically relevant distress. The aim of the study was to assess and compare the mental health of transgender youth by assigned sex at birth. A total of n = 49 adolescents (n = 29 assigned females at birth, n = 20 assigned male at birth) aged 12 to 18 years with the diagnosis of gender dysphoria according to DSM-5 were included in the study. The adolescents underwent a psychological assessment in a child and adolescent psychiatry outpatient department prior to starting gender-affirming medical treatment, completing relevant mental health questionnaires. Although no differences were found in psychiatric disorders, more externalizing problems above the clinical threshold were reported by parents in assigned female at birth (AFAB) adolescents. On the other hand, internalizing problems, both in general and within the clinical range, were found to be more prevalent in assigned male at birth (AMAB) adolescents, as indicated by self-report. Our results suggest that a comprehensive assessment of mental health in gender dysphoric adolescents is crucial for understanding the diverse range of challenges they may face and tailoring appropriate interventions to address their specific needs.
Examining the Effect of MnS Particles on the Local Deformation Behavior of 8MnCrS4-4-13 Steel by In Situ Tensile Testing and Digital Image Correlation
Authors: Qayyum Faisal , Tseng Shao-Chen , Guk Sergey , Hoffmann Frank , Chao Ching-Kong , Prahl Ulrich
Published: July 2023

In this study, the behavior of MnS particles in a steel matrix is investigated through in situ tensile testing and digital image correlation (DIC) analysis. The goal of this research is to understand the mechanical behavior of MnS inclusions based on their position in the steel matrix. To accomplish this, micro-dog bone-shaped samples are prepared, tensile tested, and analyzed. Macro-mechanical results reveal that the material yields at a stress of 350 MPa and has an ultimate tensile strength of 640 MPa, with a total elongation of 17%. For micro-mechanical analysis, scanning electron microscopy (SEM) images are taken at incremental strains and processed using DIC software to visualize the local strain evolution. The DIC analysis quantifiably demonstrates that the local strain is highest in the ferrite matrix, and while lowest in the pearlite matrix, the MnS particles and the interfaces between different materials experienced intermediate strains. The research provides new insights into the micro-mechanical deformation behavior of MnS particles in a steel matrix and has the potential to inform the optimization of the microstructure and properties of materials containing MnS inclusions.
Female Collegiate Dancers’ Physical Fitness across Their Four-Year Programs: A Prospective Analysis
Authors: Ambegaonkar Jatin P. , Hansen-Honeycutt Jena , Wiese Kelley R. , Cavanagh Catherine M. , Caswell Shane V. , Ambegaonkar Shruti J. , Martin Joel
Published: July 2023

Dance is physically demanding, requiring physical fitness (PF) that includes upper body, lower body, core fitness, and balance for successful performance. Whether PF changes as dancers advance from when they enter (freshmen) to when they graduate from their collegiate program (seniors) is unclear. We prospectively compared collegiate dancers’ freshman-to-senior PF. We recorded PF in regard to upper body strength endurance (push-ups), core strength endurance (front, left-side, right-side, and extensor plank hold times), lower body power (single leg hop—SLH—distances % height; Leg Symmetry Index: LSI = higher/lower × 100, %), and balance (anterior reach balance, % leg length, LL; LSI balance = higher/lower × 100, %) in 23 female collegiate dancers (freshman age = 18.2 ± 0.6 years). Repeated measures ANOVAs (p ≤ 0.05) were used to compare measures from freshman to senior years. Across their collegiate programs, dancers’ PF remained unchanged. Specifically, their upper body strength endurance push-up numbers (p = 0.93), their core strength endurance plank times (left: p = 0.44, right: p = 0.67, front: p = 0.60, p = 0.22), their SLH distances (left: p = 0.44, right: p = 0.85), and their symmetry (p = 0.16) stayed similar. Also, dancers’ right leg (p = 0.08) and left leg balance (p = 0.06) remained similar, with better balance symmetry (p < 0.001) in seniors. Overall, dancers’ PF did not change across their collegiate programs. Thus, female dancers’ freshman PF may be an adequate baseline reference measure when devising rehabilitation programs and determining readiness-to-return-to-activity post injury.
Performing for Better Communication: Creativity, Cognitive-Emotional Skills and Embodied Language in Primary Schools
Authors: Eschenauer Sandrine , Tsao Raphaële , Legou Thierry , Tellier Marion , André Carine , Brugnoli Isabelle , Tortel Anne , Pasquier Aurélie
Published: July 2023

While the diversity and complexity of the links between creativity and emotional skills as well as their effects on cognitive processes are now established, few approaches to implementing them in schools have been evaluated. Within the framework of the enactive paradigm, which considers the complexity and dynamics of language as a cognitive process, we study how an approach based on performative theatre can synergistically stimulate creativity (artistic, bodily and linguistic), emotional skills (identifying and understanding emotions) and executive functions (especially inhibition, cognitive flexibility and emotional control), all as components defined in the context of oral communication. Stimulating this synergy in the context of foreign language teaching may be especially beneficial for children with communication disorders. This paper presents the first results of the CELAVIE pilot study (Creativity, Empathy and Emotions in Language learning with Autism for an Inclusive Education) through a case study of a pupil with a neurodevelopmental disorder included in a 4th-grade class. The results show a progression in oral communication in English as a Foreign Language (EFL), in emotional skills and creativity.
Ontology Framework for Sustainability Evaluation of Cement–Steel-Slag-Stabilized Soft Soil Based on Life Cycle Assessment Approach
Authors: Yu Chunyang , Yuan Jia , Cui Chunyi , Zhao Jiuye , Liu Fang , Li Gang
Published: July 2023

Steel slag has become a promising supplementary cementitious material for soft soil stabilization. However, there is a lack of research on the integrated assessment of cement–steel-slag-stabilized soft soils (SCSs) from the performance, environmental, and economic perspectives. In this study, an ontology framework for the sustainable evaluation of SCSs was developed based on the life cycle assessment (LCA) approach, which combined a knowledge base with semantic web rules to achieve an automated decision design for soft soil stabilization, considering comprehensive benefits. The ontology framework was applied to a marine soft soil stabilization case to verify its scientificity and practicability and to evaluate the influence of the fineness, carbonation degree, and substitution ratio of steel slag on the sustainability of SCSs. The results show that, when compared to pure-cement-stabilized soil (S-C), using 10% and 20% of fine steel slag carbonated for 18 h (FSS-C-18h) as cement substitutes can significantly reduce carbon emissions and costs while achieving a similar strength performance as S-C, demonstrating the feasibility of steel slag as a sustainable supplementary cementitious material for soft soil stabilization.
Recording the Magnetic Field Produced by an Undersea Energy Generating Device: A Low-Cost Alternative
Authors: Luna Victor , Silva Rodolfo , Mendoza Edgar , Canales-García Iza
Published: July 2023

This work describes the characteristics of a device capable of detecting the magnetic field generated by a submerged electrical conductor. This low-cost apparatus is based on the open-source Arduino platform and offers the possibility of monitoring magnetic fields generated by undersea cables. Measuring magnetic fields generated by undersea cables facilitates the development of technologies that will harness marine energy potential. The research is based on published parameters of magnetic field values generated by existing submarine cables. A coil was built to simulate an approximate magnetic field at 10 mT. The magnetic field generated by the coil was used as a reference standard. The device developed has a measurement probe built with an array of SS49E Hall effect sensors placed in a straight line and separated 5 cm from each other. A DS18B20 temperature sensor was added to make the necessary corrections and cancel the influence of temperature during the measurements. A microSD card module was attached to store continuous magnetic field measurements. The device was adjusted under strict laboratory conditions. The functionality of the device developed was confirmed by two samplings in the sea. In these samples, the magnetic field generated by the coil was measured in the entire water column from a depth of 3 m to 150 m. Results indicate that the prototype can successfully perform the necessary functions to quantify the underwater magnetic field accurately with about 10 µT accuracy.
COVID-19 Is an Independent Risk Factor for Detrimental Invasive Fungal Disease in Patients on Veno-Venous Extracorporeal Membrane Oxygenation: A Retrospective Study
Authors: Poth Jens Martin , Schewe Jens-Christian , Lehmann Felix , Weller Johannes , Schmandt Mathias Willem , Kreyer Stefan , Muenster Stefan , Putensen Christian , Ehrentraut Stefan Felix
Published: July 2023

Invasive fungal disease (IFD) is associated with the mortality of patients on extracorporeal membrane oxygenation (ECMO). Several risk factors for IFD have been identified in patients with or without ECMO. Here, we assessed the relevance of coronavirus disease (COVID-19) for the occurrence of IFD in patients on veno-venous (V-V) ECMO for respiratory failure. In a retrospective analysis of all ECMO cases between January 2013 and December 2022 (2020–2022 for COVID-19 patients), active COVID-19 and the type, timing and duration of IFD were investigated. Demographics, hospital, ICU length of stay (LoS), duration of ECMO, days on invasive mechanical ventilation, prognostic scores (Respiratory ECMO Survival Prediction (RESP) score, Charlson Comorbidity Index (CCI), Therapeutic Intervention Scoring System (TISS)-10, Sequential Organ Failure Assessment (SOFA) score and Simplified Acute Physiology Score (SAPS)-II) and length of survival were assessed. The association of COVID-19 with IFD was investigated using propensity score matching and uni- and multivariable logistic regression analyses. We identified 814 patients supported with ECMO, and 452 patients were included in further analyses. The incidence of IFD was 4.8% and 11.0% in patients without and with COVID-19, respectively. COVID-19 status represented an independent risk factor for IFD (OR 4.30; CI 1.72–10.85; p: 0.002; multivariable regression analysis). In patients with COVID-19, 84.6% of IFD was candidemia and 15.4% represented invasive aspergillosis (IA). All of these patients died. In patients on V-V ECMO, we report that COVID-19 is an independent risk factor for IFD, which is associated with a detrimental prognosis. Further studies are needed to investigate strategies of antifungal therapy or prophylaxis in these patients.
Are the Post-COVID-19 Posttraumatic Stress Disorder (PTSD) Symptoms Justified by the Effects of COVID-19 on Brain Structure? A Systematic Review
Authors: Kotzalidis Georgios D. , Ferrara Ottavia Marianna , Margoni Stella , Ieritano Valentina , Restaino Antonio , Bernardi Evelina , Fischetti Alessia , Catinari Antonello , Monti Laura , Chieffo Daniela Pia Rosaria , Simonetti Alessio , Sani Gabriele
Published: July 2023

COVID-19 affects brain function, as deduced by the “brain fog” that is often encountered in COVID-19 patients and some cognitive impairment that is observed in many a patient in the post-COVID-19 period. Approximately one-third of patients, even when they have recovered from the acute somatic disease, continue to show posttraumatic stress disorder (PTSD) symptoms. We hypothesized that the persistent changes induced by COVID-19 on brain structure would overlap with those associated with PTSD. We performed a thorough PubMed search on 25 April 2023 using the following strategy: ((posttraumatic OR PTSD) AND COVID-19 AND (neuroimaging OR voxel OR VBM OR freesurfer OR structural OR ROI OR whole-brain OR hippocamp* OR amygd* OR “deep gray matter” OR “cortical thickness” OR caudate OR striatum OR accumbens OR putamen OR “regions of interest” OR subcortical)) OR (COVID-19 AND brain AND (voxel[ti] OR VBM[ti] OR magnetic[ti] OR resonance[ti] OR imaging[ti] OR neuroimaging[ti] OR neuroimage[ti] OR positron[ti] OR photon*[ti] OR PET[ti] OR SPET[ti] OR SPECT[ti] OR spectroscop*[ti] OR MRS[ti])), which produced 486 records and two additional records from other sources, of which 36 were found to be eligible. Alterations were identified and described and plotted against the ordinary PTSD imaging findings. Common elements were hypometabolism in the insula and caudate nucleus, reduced hippocampal volumes, and subarachnoid hemorrhages, while white matter hyperintensities were widespread in both PTSD and post-COVID-19 brain infection. The comparison partly supported our initial hypothesis. These data may contribute to further investigation of the effects of long COVID on brain structure and function.
Multidimensional Machine Learning Model to Calculate a COVID-19 Vulnerability Index
Authors: Rosero Perez Paula Andrea , Realpe Gonzalez Juan Sebastián , Salazar-Cabrera Ricardo , Restrepo David , López Diego M. , Blobel Bernd
Published: July 2023

In Colombia, the first case of COVID-19 was confirmed on 6 March 2020. On 13 March 2023, Colombia registered 6,360,780 confirmed positive cases of COVID-19, representing 12.18% of the total population. The National Administrative Department of Statistics (DANE) in Colombia published in 2020 a COVID-19 vulnerability index, which estimates the vulnerability (per city block) of being infected with COVID-19. Unfortunately, DANE did not consider multiple factors that could increase the risk of COVID-19 (in addition to demographic and health), such as environmental and mobility data (found in the related literature). The proposed multidimensional index considers variables of different types (unemployment rate, gross domestic product, citizens’ mobility, vaccination data, and climatological and spatial information) in which the incidence of COVID-19 is calculated and compared with the incidence of the COVID-19 vulnerability index provided by DANE. The collection, data preparation, modeling, and evaluation phases of the Cross-Industry Standard Process for Data Mining methodology (CRISP-DM) were considered for constructing the index. The multidimensional index was evaluated using multiple machine learning models to calculate the incidence of COVID-19 cases in the main cities of Colombia. The results showed that the best-performing model to predict the incidence of COVID-19 in Colombia is the Extra Trees Regressor algorithm, obtaining an R-squared of 0.829. This work is the first step toward a multidimensional analysis of COVID-19 risk factors, which has the potential to support decision making in public health programs. The results are also relevant for calculating vulnerability indexes for other viral diseases, such as dengue.
The Role of Radiotherapy in the Management of Vaginal Melanoma: A Literature Review with a Focus on the Potential Synergistic Role of Immunotherapy
Authors: Cuccia Francesco , D’Alessandro Salvatore , Blasi Livio , Chiantera Vito , Ferrera Giuseppe
Published: July 2023

Among the mucosal melanomas, vaginal melanomas are very rare tumors, accounting for less than 20% of melanomas arising from the female genital tract. They occur most frequently in women in post-menopausal age, but younger patients may also experience this neoplasm, mainly located in the lower third of the vagina or the anterior wall. The optimal management of this tumor remains controversial, with surgery reported as the most frequently adopted approach. However, a clear benefit of surgical treatment in terms of survival has not yet been demonstrated. Conversely, radiotherapy may represent an attractive non-invasive alternative, and there are several favorable reports of the role of radiation therapy, either delivered with photons, brachytherapy, or hadrontherapy. A wide range of techniques and fractionation regimens are reported with substantially good tolerance to the treatment, and acute G3 or higher toxicities are reported only in the case of concurrent immunotherapy. Of note, due to the rarity of the disease, there is a lack of high-level evidence for the optimal therapeutic option. In this scenario, recent studies theorize the possibility of developing combinatorial approaches of radiotherapy with immunotherapy based on cutaneous melanomas reports. In this review, we aim to summarize the evidence available in the literature supporting the role of definitive radiotherapy for vaginal melanomas, with a focus on the combination of RT with immunotherapy, in terms of optimal timing and biological rationale.