bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolomics revealed the anxiety-like behaviors led to liver injury through EGFR/PI3K/AKT/NF-κB pathway in rats
Authors: Liu Binjie , Zhang Shanshan , Sun Lizhu , Huang Lan , Zhang Rong , Liu Zhongqiu , An Lin
Published: April 2023

Psychological distress is associated with increased liver disease mortality. This association highlighted the close relationship between psychological and physical health. Thus, further work to elucidated the underlying mechanism should be carried out. In this experiment, a model of anxious rats was established by compound stress. The HPA axis situation and inflammatory factor changes in the brain of the rats were observed, which in turn were evaluated for behavioral tests and liver function, respectively. The liver metabolic profiles of the rats were characterized by liquid chromatography-mass spectrometry (LC-MS). Differential metabolites were screened by p < 0.05 and VIP > 1. Pathway enrichment analysis was performed on metabolomics data by Ingenuity Pathway Analysis (IPA). Immunofluorescence (IF), immunohistochemistry (IHC) and western blotting, were employed to detect the expression of the screened target epidermal growth factor receptor (EGFR), and verify its potential pathway of mechanism. The results show that we found liver function impairment in anxiety-like rats. Further, 61 differential metabolites in control and anxiety groups were screened by using metabolomics (p < 0.05, VIP > 1). IPA analysis revealed the key target EGFR which was also identified from the HPA axis. We further found that anxiety-like rats may cause liver injury through EFGR/PI3K/AKT/NF-κB pathway, and then lead to the production of inflammatory factors in the liver. This study demonstrated that the activation of HPA axis in anxiety-like rats led to phosphorylation of EGFR, which finally contributed to liver injury through the EGFR/PI3K/AKT/NF-κB pathway. This finding provided novel evidence for the deleterious effects of psychological problems on physical health.
Clicking Through the Clickstream: A Novel Statistical Modeling Approach to Improve Information Usage of Clickstream Data by Ecommerce Entities
Authors: Allenbrand Corban
Published: April 2023

Success or failure of an ecommerce platform is often reduced to its ability to maximize the conversion rate of its visitors. This is commonly regarded as the capacity to induce a purchase from a visitor. Visitors possess individual characteristics, histories, and objectives which complicates the choice of what features of a web system solves the conversion maximization problem. Modern web technology has made clickstream data accessible allowing a complete record of a visitor’s actions on a website to be analyzed. What remains poorly constrained is what parts of the clickstream data are meaningful information and what parts are accidental. In this research clickstream data from an online retailer was examined to provide answers to the previous questions with statistical modeling. A conceptual model was developed from which several hypotheses on the nature of clickstream relationships were posited. A discrete choice logit model was developed which showed that the content of a website, the history of website use, and the exit rate of pages visited had marginal effects on derived utility for the visitor. Exit rate and bounce rate were modeled as beta distributed random variables. It was found that exit rate and its variability for pages visited by visitors was associated with site content, site quality, prior visitor history on the site, and technological preferences of the visitor. Bounce rate was also found to be influenced by the same factors but were in a direction opposite to the registered hypotheses. Most findings supported that clickstream data is open to statistical modeling with interpretable and comprehensible models.
“I literally had no support”: Barriers and facilitators to supporting the psychosocial wellbeing of young people with mental illness in Tasmania, Australia
Authors: Savaglio Melissa , Yap Marie , Smith Toni , Vincent Ash , Skouteris Helen
Published: April 2023

Background There has been limited focus on understanding the barriers and facilitators to meeting the broader psychosocial needs of young people with mental illness, from the perspectives of young people themselves. This knowledge is required to advance the local evidence base and inform service design and development. Therefore, the aim of this qualitative study was to explore young people’s (10–25 years) and carers’ experiences of mental health services, focusing on the barriers and facilitators to services supporting young people’s psychosocial functioning.Method Young people with living experience of mental illness were involved in all stages of this research. Semi-structured interviews were conducted with 32 young people aged 10–25 years with experience of mental illness, and 29 carers (12 were parent-child dyads). Qualitative analysis was guided by the Social-Ecological Framework to identify barriers and facilitators at the individual (young person/carer level), interpersonal, and service/systemic level.Results Young people and carers identified eight barriers and six facilitators across the various levels. Barriers included, at the individual level: (1) the complexity of young people’s psychosocial needs and (2) lack of awareness/knowledge of services available; at the interpersonal level: (3) negative experiences with adults and (4) fragmented communication between services and family; and at the systemic level: (5) lack of services; (6) long waiting periods; (7) limited service accessibility; and (8) the missing middle. Facilitators included, at the individual level: (1) education for carers; the interpersonal level: (2) positive therapeutic relationships and (3) carer advocacy/support; and systemic level: (4) flexible or responsive services, (5) services that address the psychosocial factors; and (6) safe service environments.Conclusions This study identified lived-experience recommendations for public mental health policy and practice, including service design and development. To better enhance their psychosocial functioning, young people and carers want lived-experience workers to provide practical wrap-around support, and they want mental health services that integrate health and social care, and are flexible, responsive and safe. These findings will inform the co-design and development of a new community-based youth psychosocial service to support the psychosocial wellbeing of young people experiencing severe mental illness.
In Situ Assembly of Bioresorbable Organic Bioelectronics in the Brain
Authors: Hjort Martin , Mousa Abdelrazek H. , Bliman David , Shameem Muhammad Anwar , Hellman Karin , Yadav Amit Singh , Ekström Peter , Ek Fredrik , Olsson Roger
Published: April 2023

Bioelectronics has the potential to complement classical therapies in nonchronic treatments, such as immunotherapy and cancer. In addition to functionality, minimally invasive implantation methods and bioresorbable materials are central to nonchronic treatments. The latter is to avoid the need for surgical removal after disease relief. Self-organizing substrate-free organic electrodes have the potential to meet these criteria and the need for seamless integration into dynamic biological systems in a way that is not possible for classical rigid solid-state electronics. We have developed a general approach that is not dependent on specific external or endogenous triggers to assemble bioresorbable electrodes from water-dispersed nanoparticles in the brain. To control precise localization, conductive structures were placed in the targeted area using a capillary thinner than a human hair. An optional auxiliary module grew dendrites from the installed organic electrode to seamlessly embed neurons. This module also modified the conductive structure’s volume properties, such as functionality and biocompatibility while displaying brain matched shear modulus. These soft microdendritic electrodes set off a controlled cellular response in the brain when relaying external stimuli. The materials were well tolerated, and the initial inflammation in the brain caused by the implantation was resolved, showing no tissue damage after bioresorption. These findings initiate the discovery of in vivo assembled, fully integrated, transient organic bioelectronics within cellular structures that can be used for nonchronic treatments.
Production and composition of Extracellular Polymeric Substances (EPS) by a unicellular strain and natural colonies of Microcystis: impact of salinity and nutrient stress
Authors: Reignier Océane , Bormans Myriam , Marchand Laetitia , Sinquin Corinne , Amzil Zouher , Zykwinska Agata , Briand Enora
Published: April 2023

The transfer of toxic cyanobacterial Microcystis blooms from freshwater to estuaries constitutes a serious environmental problem worldwide that is expected to expand in scale and intensity with anthropogenic and climate change. The formation and maintenance of Microcystis in colonial form is conditioned to the presence of Extracellular Polymeric Substances (EPS). In an attempt to better understand how mucilaginous colonial form may evolved under environmental stress condition, we studied and compared the production and the composition of EPS fractions (attached and free) from natural colonies of a Microcystis bloom and from a unicellular M. aeruginosa strain under salinity and nutrient stress (representing a land-sea continuum). Our results highlighted a greater production of EPS from the natural colonies of Microcystis than the unicellular one without or under stress condition. In comparison to the unicellular Microcystis, EPS produced by the colonial form were characterized by high molecular weight polysaccharides which were enriched in uronic acids and hexosamines, notably for the free fraction in response to increased salinities. This complex extracellular matrix gives the cells the ability to aggregate and allows the colonial cyanobacterial population to cope with osmotic shock.
Issues Related to IUCD Discontinuation Rates Among IUCD Users in Kembata Tembaro Zone, Southern Ethiopia
Authors: Yoseph Tessema
Published: April 2023

Background
Intrauterine contraceptive devices (IUCDs) are made of T-shaped plastic and are inserted into women’s uteruses to prevent unwanted pregnancies. Despite the improvement in the availability and utilisation of intrauterine contraceptive devices, discontinuation is becoming a public health concern. A significant proportion of women discontinue the method before its due date, which poses a concern in the health system, and its consequences may lead to the failure of a programme. As a result, the purpose of this study was to investigate the issues related to IUCD discontinuation rates among IUCD users IN twelve months ago in Kembata Tembaro Zone, Southern Ethiopia, in 2022.
Methods
A community-based, cross-sectional study was conducted. 415 women who had inserted an IUD from July 2021 to June 2022 in the Kembata Tembaro Zone were selected using a multistage sampling technique included in the analysis. This study's data collection instruments were pre-tested structured questionnaires. The coded data was entered into Epi Data version 4.6 and exported to SPSS version 25 for analysis. Finally, binary logistic regression analysis was carried out to identify independently associated factors and odds ratios at a 95% confidence interval with a significance level of p-value less than 0.05.
Results
Analysis revealed that 21% (95% CI 16.5–22.8) of women discontinued the use of their intrauterine contraceptive device in the last year. Issues like not being counselled about the intrauterine contraceptive device before insertion [AOR = 3.7; 95% CI: 1.23–7.30], not being appointed for follow-up [AOR = 2.8; 95% CI: 1.12–6.70], being married [AOR = 2.9; 95% CI: 1.35–6.23], and needing to have more children [AOR = 3.2; 95% CI: 1.5–7.0]
Conclusions
The findings of this study conclude that the overall magnitude of the IUCD discontinuation rates in the study area were found to be high when compared with different studies conducted in Ethiopia. Many of the factors that are attributed to the high magnitude of IUCD discontinuation are changeable. Appropriate counseling prior to insertion, including an appointment for follow-up visits, the preference to have more children, and marital status for the choice of service, will improve the continuation rate of IUCD.
Effect of Post-COVID Face-to-Face learning Mode on Endodontic Students’ Satisfaction and Exam Performance Compared to Hybrid Learning Mode during the pandemic; a Single-centre Retrospective Study.
Authors: Elemam Ranya , Elsewify Tarek , Eid Bassem
Published: April 2023

Aim
This study compared the perceptions and exam performance of third-year undergraduates in endodontic courses. The study analyzed hybrid training during COVID-19 and Face-to-Face teaching post-COVID-19 to identify the most effective teaching methods.
Materials and Methods
This study was a single-centre retrospectively analyzed endodontic courses delivered to third-year dental students in 2021 and 2022 at the Gulf Medical University, College of Dentistry. Student perceptions of the hybrid and F2F courses were obtained through a satisfaction questionnaire administered by the University's Quality Assurance Office at the end of each course. Student performance was measured by analyzing exam grades for both teaching modes.
Results
Total of 75 students took the hybrid course while 77 received the F2F course. The number of respondents to the questionnaire was significantly higher for the F2F group (81% vs. 31.5%, p ≤ 0.05). The hybrid course received a satisfaction score of 100% compared to 94.54% for F2F. Student performance in the hybrid group was comparable to the F2F group, with mid- and end-of-year course marks ranging from 64.67% to 71.46% and 63.93% to 65.79%, respectively (p ≤ 0.05). These findings suggest that the hybrid model did not negatively affect student performance.
Conclusion.
In conclusion, the hybrid learning approach was well-received and produced similar endodontic exam marks to F2F teaching. This suggests that combining e-learning with F2F teaching can maintain the quality of education. The absence of differences between the groups dismisses concerns that endodontic education suffered due to the pandemic.
WITHDRAWN: Control Algorithm Validation Using PLC based Hardware Test-Bench for Stable Microgrid Automation
Authors: Mehta Sahil , Basak Prasenjit
Published: April 2023

With the advancements in automation-based technology in engineering applications, programmable logic controller (PLC) has gained much attention. These PLCs possess real-time on-field applicability, reliable operation, long life, robust behavior and quick action in response to various inputs, multiple decision-making variables, harsh environmental conditions, etc. However, unlike the simulation software, tool boxes, and other setups, a robust and reconfigurable PLC can be employed to validate control algorithms for microgrids considering any geographical and environmental conditions. Thus, this paper proposes a PLC-based hardware test bench prototype as an effective solution for control algorithm validation aiming at power management problems and stable microgrid automation. The critical contribution of this paper includes the design and fabrication of the controller test bench prototype, abstracting it as the microgrid central controller (MGCC). The energy management system for the microgrid model with the fuzzy logic-based controller has been developed using MATLAB Simulink software. The performance of the proposed controller test bench is analyzed considering perturbations due to reduced renewable resource contribution. The results validate the proposed controller’s control algorithms, which can ensure the stable operation of the microgrid.
A Simple Framework for Likely Climate Projections Applied to Tropical Width
Authors: Baldassare Daniel , Reichler Thomas
Published: April 2023

Confronted with the difficult task of choosing between a wide range of climate models and emission scenarios, scientists are often left questioning which models and scenarios are most representative of our future climate. Currently, most climate projections use the highest emission scenario and an evenly weighted ensemble of all available climate models despite substantial evidence that the continuously rising emissions in the high emission scenario are unrealistic, and that some models are more reliable than others. As a result, unlikely climate projections have been presented as our likely future. Here, we present a simple and easy to use framework for creating projections of our likely future climate using available model data. The framework involves three measures: selecting the most likely emission scenario, choosing the most reliable models, and debiasing against reanalysis data. To demonstrate our framework and its components, we estimate future changes in tropical width, a hydrologically important climate feature. Our projections show that the likely tropical expansion is roughly half of previous estimates. This framework can be easily applied to other climate features, allowing for better estimates of likely future conditions.
Neuroanatomical Predictors of Alcohol Consumption in Adolescents via in vivo Structural Imaging: A Systematic Review of Longitudinal Studies
Authors: Honarvar Faraz , Arfaie Saman , Edalati Hanie , Ghasroddashti Arashk , Solgi Arad , Mashayekhi Mohammad Sadegh , Mofatteh Mohammad , Ren Lily Yuxi , Kwan Angela Tian Hui , Keramatian Kamyar
Published: April 2023

Aims
This study aimed to systematically review the literature on neuroanatomical predictors of future problematic drinking in adolescents.
Methods
Using PRISMA guidelines, a systematic review was conducted to evaluate neuroanatomical predictors of problematic drinking in adolescents. Embase, MEDLINE, and PsycINFO databases were searched from inception to January 6th, 2023. Studies were included if they were original, had a prospective design, had a sample size of at least 12, had a follow-up period of at least one year, had at least one structural neuroimaging scan before 18 with no prior alcohol use, and had alcohol use as the primary outcome. Studies were excluded if: had animals only; and were not in English. Risk of bias was conducted using the CASP tool.
Results
Out of 1,412 studies identified, 19 studies met the criteria, consisting of eleven gray matter (n = 4,040), five white matter (n = 319), and three assessing both (n = 3,608). Neuroanatomical predictors of future problematic drinking in adolescents were reported to be distributed across various brain regions such as the orbitofrontal cortex and paralimbic regions. However, the findings were largely heterogeneous.
Conclusions
This is the first systematic review to map out the existing literature on neuroanatomical predictors of problematic drinking in adolescents. Future research should focus on the aforementioned regions to determine their role in predicting future problematic drinking with more certainty.
Evidence for Involvement of the Alcohol Consumption WDPCP Gene in Lipid Metabolism, and Liver Cirrhosis
Authors: Pazoki Raha , O’Farrel Felix , Aleyakpo Benjamin , Mustafa Rima , Jiang Xiyun , Pinto Rui , Elliott Paul , Tzoulaki Ioanna , Abbas Dehghan , Loh Samantha , Barclay Jeff , Martins Luis
Published: April 2023

Alcohol consumption continues to cause a significant health burden globally. The advent of genome-wide association studies has unraveled many genetic loci associated with alcohol consumption. However, biological effects of these loci and pathways involved in alcohol consumption and its health consequences such as alcohol liver disease (ALD) remain to be elucidated. We combined human studies with model organisms Drosophila melanogaster and Caenorhabditis elegans to shed light on molecular mechanisms underlying alcohol consumption and the health outcomes caused by alcohol intake. Using genetics and metabolite data within the Airwave study, a longitudinal study to investigate the health of employees of police forces in the UK, we performed several analyses to identify changes in circulating metabolites that are triggered by alcohol consumption and found an enrichment of the alcohol-associated metabolites within the linoleic acid (LNA) and alpha-linolenic acid (ALA) metabolism pathways. We identified evidence of a potential causal relationship between alcohol consumption with several triradylglycerols (TAGs) and diradylglycerols (DAGs), a fatty ester (CAR DC18:1), an sphingomyelin (SM 40:2;O2), and an alkaloid (Piperine). We selected a set of genes annotated to genetic variants that (1) are known to be implicated in alcohol consumption, (2) are linked to liver function, and (3) are associated with the expression (cis-eQTL) of their annotated genes. We used mutations and/or RNA interference (RNAi) to suppress the expression of these genes in C. elegans and Drosophila. Testing the differences in locomotion of C. elegans showed that RNAi knockdown of ACTR1B and MAPT reduced locomotion rate in C. elegans after exposure to ethanol. We showed that RNAi knockdown of several genes (WDPCP, TENM2, GPN1, ARPC1B, SCN8A) in Drosophila changed the sedative effect of ethanol whilst RNAi knockdown of TENM2 reduced ethanol consumption. We also investigated alcohol-induced changes in TAG levels in Drosophila and demonstrated that RNAi knockdown of WDPCP, TENM2 and GPN1 reduce TAG levels. Finally, using publicly available human data, we showed that gene expression of WDPCP is linked to liver fibrosis and liver cirrhosis. Our results underline the impact of alcohol consumption on the metabolism of lipids and pinpoint WDPCP as a gene with a potential impact on lipid accumulation upon exposure to ethanol suggesting a possible pathway to ALD.
Trabajadores transfronterizos o commuters internacionales en la frontera México-Estados Unidos: evolución e importancia económica
Authors: Orraca Romano Pedro P.
Published: April 2023

Este artículo analiza la evolución de los trabajadores transfronterizos que residen en México y laboran en Estados Unidos y cuantifica su importancia económica. Con base en información censal y la ENIGH se estiman modelos de regresión lineal y Tobit. Durante el periodo 2000-2020, el número de transfronterizos aumentó ligeramente y la participación de los estadounidenses dentro de este grupo se incrementó sustancialmente. En 2020, los transfronterizos aportaron 7.3% de la masa salarial y ejercieron 7.5% del gasto total de los municipios del norte de México. Además, el tener a un transfronterizo en el hogar se asocia con un gasto 56.8% mayor en México y 283.1% mayor en Estados Unidos. El ser transfronterizo representa para las personas del norte de México con posibilidades de laborar en Estados Unidos una estrategia efectiva para maximizar su ingreso y acceder a un mejor nivel de vida.
THE TECHNIQUE OF BUILDING A SUSTAINABLE PROTECTION SYSTEM BASED ON ADVERSARIAL MACHINE LEARNING IN 6G WIRELESS NETWORKS
Authors:   Laboratory of Digital Solutions and Big Data Analytics, Orenburg State University, Legashev Leonid , Grishina Lyubov ,   Laboratory of Digital Solutions and Big Data Analytics, Orenburg State University
Published: April 2023

Abstract The purpose of research is to develop the technique of analytical processing of big data of services and applications in the new generation communication networks to detect cybersecurity incidents and build sustainable protection systems based on adversarial machine learning. The methods of research: Analysis of modern methods of machine learning and neural network technologies, synthesis and formalization of algorithms for adversarial attacks on machine learning models. Scientific novelty: a technique for analytical processing of emulated data of services and applications for detecting cybersecurity incidents is presented, which provides a groundwork in the field of research into the security issues of complex intelligent services and applications in the infrastructure of wireless networks of the next generation. The result of research: The article proposes a technique of building a sustainable protection system against adversarial attacks in wireless ad hoc networks of the next generation. The main types of adversarial attacks, including poisoning attacks and evasion attacks, are formalized, and methods for generating adversarial examples on tabular, textual, and visual data are described. Several scenarios were generated and exploratory analysis of datasets was carried out using the DeepMIMO emulator. Potential application problems of binary classification and prediction of signal attenuation between a user and a base station for adversarial attacks are presented. The algorithmization of the processes of building and training a sustainable protection system against adversarial attacks in wireless networks of the next generation is presented on the example of emulated data
International community and communitarianism in international law
Authors: Widłak Tomasz
Published: April 2023

This essay proposes that one of the most notable achievements of Professor Janusz Gilas’s work is the development of community-oriented thinking in Polish international legal scholarship. His use of the term “international community” is not merely figurative and does not boil down to distinguishing another doctrinal concept. The article further develops the legal debate around the concept of international community by proposing that it should be grounded in philosophical and political communitarianism. After briefly discussing Gilas’s contribution and its theoretical underpinnings in the form of the sociological theory by Ferdinand Tönnies and the achievements of the English School of international relations, this article undertakes to present communitarianism as a social and political philosophy and its relation to the concept of the international community, its theoretical alternatives, and their base values. Having clarified the possible communitarian interpretation of the international community, the author turns to the question of the possibility of communitarian international law. The article concludes by reinterpreting the thesis that international law is viable and conceivable only as community law, oriented toward the common good.
Vía antropológica de acceso al conocimiento de Dios
Authors: Aznar Francisco
Published: April 2023

La idea de Dios se ha ido debilitando en el imaginario colectivo y cada vez son menos los que la siguen. No cabe duda de que la revelación positiva de lo divino ha quedado únicamente para aquellos que dicen creer, pero tradicionalmente se dieron unas vías de acceso a lo divino que fueron jalonadas desde el quehacer filosófico. El artículo pretende recorrer ese camino desde postulados cosmológicos y antropológicos, con la intención de establecer un puente de diálogo estrictamente racional.
Panax notoginseng Saponins Alleviate LPS-induced Fibrosis of HK-2 Cells
by Inhibiting the Activation of NLRP3 Inflammasome and Pyroptosis
Authors: Xie Jing , Ma Xin , Li Xueying , Mao Nan , Ren Sichong , Fan Junming
Published: April 2023

Background:

Renal fibrosis is related to impaired kidney function and can eventually
lead to end-stage renal disease, for which no effective treatment is available. Panax notoginseng
saponins (PNS), as a commonly used traditional Chinese medicine, is considered a possible alternative
for the treatment of fibrosis.

Objective:

The purpose of the present study was to investigate the effects and possible mechanisms
of PNS on renal fibrosis.

Methods:

HK-2 cells were used to induce renal fibrosis cell model by lipopolysaccharide (LPS),
and the cytotoxicity of PNS on HK-2 cells was investigated. Cell damage, pyroptosis, and fibrosis
were analyzed to investigate the effects of PNS on LPS-induced HK-2 cells. NLRP3 agonist Nigericin
was used further to explore the inhibitory effect of PNS on LPS-induced pyroptosis so as
to clarify the possible mechanism of PNS on renal fibrosis.

Results:

PNS had no cytotoxicity on HK-2 cells, and could reduce the apoptosis and the release of
lactate dehydrogenase (LDH) and inflammatory cytokines of LPS-induced HK-2 cells, showing an
alleviating effect on cell damage. PNS also reduced the expression of pyroptosis proteins NLRP3,
IL-1β, IL-18, and Caspase-1, as well as fibrosis proteins α-SMA, collagen Ⅰ and p-Smad3/Smad3,
which showed an inhibitory effect on LPS-induced pyroptosis and fibrosis. In addition, LPSinduced
cell damage, pyroptosis, and fibrosis were aggravated after Nigericin treatment, while
PNS alleviated the aggravation caused by Nigericin.

Conclusion:

PNS inhibited pyroptosis by inhibiting the activation of NLRP3 inflammasome in
LPS-induced HK-2 cells, which ultimately alleviated renal fibrosis and played a good role in the
treatment of kidney diseases.
ATF3 Modulates the Proliferation, Migration, and Apoptosis of
Synovial Fibroblasts after Arthroscopy by Promoting RGS1
Transcription
Authors: He Xiao , Deng Lili , Zou Kang , Tian Yun , Tang Xianzhe
Published: April 2023

Background:

Osteoarthritis (OA) is a degenerative joint disease involving
both cartilage and synovium. Activating transcription factor 3 (ATF3) and regulator of G
protein signaling 1 (RGS1) have been reported to be up-regulated in OA. However, little
is known regarding the relationship between these two genes and the mechanism of this
relationship in OA development. Therefore, the present study explores the mechanism of
ATF3-mediated RGS1 in the proliferation, migration, and apoptosis of synovial
fibroblasts.

Methods:

After the OA cell model was constructed with TGF-β1 induction, human
fibroblast-like synoviocytes (HFLSs) were transfected with ATF3 shRNA or RGS1
shRNA alone or co-transfected with ATF3 shRNA and pcDNA3.1-RGS1. Then,
proliferation, migration, apoptosis, and the expression of ATF3, RGS1, α-SMA, BCL-2,
caspase3, and cleaved-caspase3 were measured. Meanwhile, the potential relationship
between ATF3 and RGS1 was predicted and validated.

Results and Discussion:

Analysis of the GSE185059 dataset suggested that RGS1
was up-regulated in OA synovial fluid exosomes. Moreover, ATF3 and RGS1 were both
highly expressed in TGF-β1-induced HFLSs. Transfection of ATF3 shRNA or RGS1
shRNA significantly reduced proliferation and migration and promoted apoptosis of TGF-
β1-induced HFLSs. Mechanistically, ATF3 bound to the RGS1 promoter and elevated
RGS1 expression. Silencing ATF3 repressed proliferation and migration and enhanced
apoptosis of TGF-β1-induced HFLSs by down-regulating RGS1.

Conclusion:

ATF3 binds to the RGS1 promoter and enhances RGS1 expression to
accelerate cell proliferation and block cell apoptosis in TGF-β1-induced synovial
fibroblasts.
Synthetic and Mechanistic Investigations in cis-3-(Substituted acetoxy)
azetidin-2-ones from cis-3-Hydroxyazetidin-2-ones: Potential
Synthons for Pharmacophoric Hybridized Molecules
Authors: Bhalla Aman , Modi Garima , Kaur Jaswinder , Kumar Pankaj , Bari Shamsher S.
Published: April 2023

The present work describes the synthesis of cis-3-(substituted acetoxy)azetidin-2-ones from
cis-3-hydroxyazetidin-2-ones. Two different routes have been investigated for the substitution at the
C-3 position of the azetidin-2-ones. Method A involves the use of acetyl chloride XCOCl in the presence
of pyridine and method B consists of using appropriate acid XCOOH in a catalytic amount of
DMAP which was found to be the best to furnish the target azetidin-2-one. All the newly synthesized
compounds were characterized on the basis of various spectroscopic techniques (FT-IR, 1H NMR, 13C
NMR, and elemental analysis). Two different routes have been investigated for the substitution at the
C-3 position of the azetidin-2-ones.
Knockdown of VASH2 Inhibits the Stemness and EMT Process by Regulating
ZEB2 in Colorectal Cancer
Authors: Shayimu Paerhati , Yin Cheng , Zeng Xiangyue , Jiapaer Rexida
Published: April 2023

Introduction:

VASH2 is associated with the malignant progression of a variety of tumors, but
the role and mechanism of VASH2 in colorectal cancer are still unclear.

Methods:

We analyzed the expression of VASH2 in colorectal cancer from the TCGA database and also
analyzed the relationship between VASH2 expression and survival of colorectal cancer patients in the
PrognoScan database. We verified the role of VASH2 in colorectal cancer through transfecting si-VASH2
into colorectal cancer cells and detecting cell viability by CCK8, cell migration by wound healing assay,
and cell invasion by Transwell assay. ZEB2, Vimentin, and E- cadherin protein expression were examined
by Western-Blot assay. Cell sphere-forming ability was determined by sphere formation assay, and
we further confirmed the mechanism of VASH2 in colorectal cancer progression by rescue assays.

Results:

Colorectal cancer has a high expression of VASH2, and its expression is associated with a poorer
patient survival rate. The vitality, migration, invasion, EMT, and tumor stemness of colorectal cancer
cells were all decreased by VASH2 knockdown. These alternations were attenuated by ZEB2 overexpression.

Conclusion:

Our experiments confirmed that VASH2 affects colorectal cancer cell proliferation, migration,
invasion, EMT, and seed bovine stemness by regulating ZEB2 expression.
The Effectiveness of UV-C Light for Inactivating Listeria monocytogenes Bacteria and its Impact on Apple Juice
Authors: Tirono Mokhamad
Published: April 2023

Background:

Apple juice is useful for preventing cardiovascular disease, degenerative disease, and cancer. The supply of apple juice is often contaminated with microbes due to raw materials and different processing methods. This study aimed to determine the effectiveness of inactivating L. monocytogenes bacteria using UV-C light by observing its impact on vitamin C content, pH, and color of apple juice.

Methods:

The research sample was Manalagi apple juice contaminated with L. monocytogenes bacteria. The treatment used UV-C with a wavelength of 185-280 nm and the optimum intensity at 256 nm. The light intensity used for the treatment was 0-125 mW/cm2, with treatment times of 30 minutes and 60 minutes.

Results:

The results showed that the inactivation of bacteria with a light intensity of 125 mW/cm2 for 30 minutes reduced the number of bacteria by 0.49 ± 0.02 log10, while the inactivation for 60 minutes was able to reduce the number of bacteria by 1.01 ± 0.02 log10. Treatment using UV-C reduced the content of vitamin C, raised the pH, and changed the color of apple juice.

Conclusion:

Treatment using UV-C with higher intensity was effective in reducing the number of large bacteria but had the potential to decrease the vitamin C content and change the color of apple juice to brown. Treatment using UV-C with an intensity of 100 and 125 mW/cm2 affected the smell of apple juice.
Obstacle Avoidance Path Planning of 7-DOF Redundant Manipulator
Based on Improved Ant Colony Optimization
Authors: Wang Shuaixiu , Wang Wenjie , Cao Yuting , Luo Yang , Wang Xiaohua
Published: April 2023

Background:

Obstacle avoidance path planning is an important parameter of robot manipulators.
Path planning can directly affect the working efficiency of the manipulator.

Objective:

This study aims to summarize the optimization design method from a large number of literature
and propose a new optimization design method to make the planned obstacle avoidance path of the
manipulator shorter and smoother.

Methods:

The forward and inverse kinematics of the redundant manipulator is solved. Secondly, the obstacle
and the robot manipulator envelope are simplified for collision detection. Then, the ant colony algorithm
is improved by adding an obstacle environment to the construction of the heuristic function and
dynamically adjusting the heuristic function factor to make the shortest path distance planned by the algorithm.
Finally, the worst ant colony is added to the pheromone update to avoid the algorithm falling
into the local optimal solution.

Results:

Through experiments and comparative studies, the optimized design process shows that the
path planned by the improved ant colony algorithm has obvious advantages of shorter path distance and
smoother path distance, which verifies the rationality of the improved algorithm.

Conclusion:

This method optimizes the convergence speed of the ant colony algorithm and avoids the
ant colony algorithm from falling into the local optimal solution, which is of great significance for improving
the obstacle avoidance path planning problem of a redundant manipulator with a degree of
freedom.
Identification of Competing Endogenous RNA Networks and Potential
Therapeutic Drugs for Gastroesophageal Reflux Disease
Authors: Su Xin , Zhang Danping , Zhu Hong
Published: April 2023

Background:

The diagnosis and cure rate of gastroesophageal reflux disease (GERD) is low, and its pathogenesis remains unclear.

Aims:

In this study, we aimed to construct the competing endogenous RNA (ceRNA) of GERD by bioinformatics and explore the potential therapeutic drugs for GERD.

Methods:

The differentially expressed genes (DEGs) were got by limma package and visualized by ggplot2 and pheatmap package in R version 4.1.3 from the GSE63401 gene microarray data. The pro-tein-protein interaction (PPI) network was constructed by the STRING database. The hub genes and subnetwork modules were obtained by Cytoscape software. The microRNA and lncRNA were pre-dicted by the miRTarBase and miRNet databases to construct the ceRNA network. Potential drugs tar-geting DEGs were screened by the CMAP database.

Results:

In this study, a total of 571 GEDs were obtained, including 462 up-regulated genes and 109 down-regulated genes, which mainly participated in the leukocyte migration process and the cytokine-cytokine receptor interaction pathway. Seventeen miRNAs and three key lncRNAs involved in regulat-ing hub genes were screened through miRTarBase and miRNet databases. Finally, nine ceRNA net-works were obtained, including TTN-AS1/NEAT1/XIST - miR-139-5p - CXCR4 axis, TTN-AS1/NEAT1/XIST - miR-185-5p - IL1B axis and TTN-AS1/NEAT1/XIST - miR-495-3p - LYN axis. In addition, five potential drugs (Pimavanserin, BMS-182874, CL-218872, Losartan, and Laropiprant) for GERD were obtained through the CMAP database.

Conclusion:

We constructed nine GERD-related ceRNA networks and screened five potential thera-peutic drugs for GERD, providing a theoretical basis for further research on the pathogenesis of GERD.
Intangible Cultural Heritage and Tourism in China
Authors: Su Junjie
Published: April 2023

This book examines the relationship between intangible cultural heritage (ICH) and tourism, taking as a focus the ICH at the World Cultural Heritage site in Lijiang, China. It explores the tensions between authenticity and commodification and provides theoretical guidelines for developing a sustainable ICH tourism from a people-based approach.
Reliability of Medical Information Provided by ChatGPT: Assessment Against Clinical Guidelines and Patient Information Quality Instrument (Preprint)
Authors: Walker Harriet Louise , Ghani Shahi , Kuemmerli Christoph , Nebiker Christian Andreas , Müller Beat Peter , Raptis Dimitri Aristotle , Staubli Sebastian Manuel
Published: April 2023

BACKGROUND

ChatGPT-4 is the latest release of a novel artificial intelligence (AI) chatbot able to answer freely formulated and complex questions. In the near future, ChatGPT could become the new standard for health care professionals and patients to access medical information. However, little is known about the quality of medical information provided by the AI.

OBJECTIVE

We aimed to assess the reliability of medical information provided by ChatGPT.

METHODS

Medical information provided by ChatGPT-4 on the 5 hepato-pancreatico-biliary (HPB) conditions with the highest global disease burden was measured with the Ensuring Quality Information for Patients (EQIP) tool. The EQIP tool is used to measure the quality of internet-available information and consists of 36 items that are divided into 3 subsections. In addition, 5 guideline recommendations per analyzed condition were rephrased as questions and input to ChatGPT, and agreement between the guidelines and the AI answer was measured by 2 authors independently. All queries were repeated 3 times to measure the internal consistency of ChatGPT.

RESULTS

Five conditions were identified (gallstone disease, pancreatitis, liver cirrhosis, pancreatic cancer, and hepatocellular carcinoma). The median EQIP score across all conditions was 16 (IQR 14.5-18) for the total of 36 items. Divided by subsection, median scores for content, identification, and structure data were 10 (IQR 9.5-12.5), 1 (IQR 1-1), and 4 (IQR 4-5), respectively. Agreement between guideline recommendations and answers provided by ChatGPT was 60% (15/25). Interrater agreement as measured by the Fleiss κ was 0.78 (P<.001), indicating substantial agreement. Internal consistency of the answers provided by ChatGPT was 100%.

CONCLUSIONS

ChatGPT provides medical information of comparable quality to available static internet information. Although currently of limited quality, large language models could become the future standard for patients and health care professionals to gather medical information.
Topic Modeling on Online News.Portal Using Latent Dirichlet Allocation (LDA)
Authors: Fahlevvi Mohammad Rezza , SN Azhari
Published: April 2023

The amount of News displayed on online news portals. Often does not indicate the topic being discussed, but the News can be read and analyzed. You can find the main issues and trends in the News being discussed. It would be best if you had a quick and efficient way to find trending topics in the News. One of the methods that can be used to solve this problem is topic modeling. Theme modeling is necessary to allow users to easily and quickly understand modern themes' development. One of the algorithms in topic modeling is the Latent Dirichlet Allocation (LDA). This research stage begins with data collection, preprocessing, n-gram formation, dictionary representation, weighting, topic model validation, topic model formation, and topic modeling results.            Based on the results of the topic evaluation, the. The best value of topic modeling using coherence was related to the number of passes. The number of topics produced 20 keys, five cases with a 0.53 coherence value. It can be said to be relatively stable based on the standard coherence value.
Unsupervised Text Style Transfer for Authorship Obfuscation in Bahasa Indonesia
Authors: Sari Yunita , Al Faridzi Fadhlan Pasyah
Published: April 2023

Authorship attribution is an NLP task to identify the author of a text based on stylometric analysis. On the other hand, authorship obfuscation aims to protect against authorship attribution by modifying a text’s style. The main challenge in authorship obfuscation is how to keep the content of the text despite the text modification. In this research, we are applying text style transfer methods for modifying the writing style while preserving the content of the input text. We implemented two unsupervised text style transfer: dictionary-based and back translation methods to change the formality level of the text. Experiment results shows that the back-translation method outperformed the dictionary-based method. The authorship attribution performance decreased up to 16.15% and 23.66% on F1-score for 3 and 10 authors respectively using back-translation. While for dictionary-based method the F1-score dropped up to 1.99% and 11.56% for 3 and 10 authors respectively. Evaluation on sensibleness and soundness factors show that the back-translation method can preserve the semantic of the obfuscated texts. Moreover, the modified texts are well-formed and inconspicuous.
Klasifikasi Suara Paru-Paru Berdasarkan Ciri MFCC
Authors: Rafiqo Dody , Suyanto Yohanes , Atmaji Catur
Published: April 2023

The lungs are an important organ in the human respiratory system, which functions to exchange carbon dioxide from the blood with oxygen in the air. Detection of respiratory disorders and lung disorders can be done in various ways; view medical records, physical examination, detection by x-ray and also auscultation of breathing. Digital signal processing can be used as a method to detect lung disorders based on the sound produced. In this study, lung sounds were classified into normal, crackle, wheeze, and crackle-wheeze classes using the Mel Frequency Cepstral Coefficient (MFCC) and Convolutional Neural Network (CNN) methods.Observations were made by varying the MFCC feature extraction using MFCC 8 and 13 coefficients, the number of frames are 50 and 60, and the width of the frames used was 0,1, 0,15 and 0,2 seconds. The result of feature extraction is then applied to the CNN classification system, and the confusion matrix is used to get the accuracy and precision values. The highest accuracy and precision values were obtained at 71,85% and 65,70% on the MFCC 13 coefficient with an average of 71,18%. Based on these results, the system that has been created can classify normal lung sounds, crackle, wheeze and crackle-wheeze quite well.
An Assessment on Work Engagement and Organizational Learning Capability among Educators
Authors: Cordova Jr. Nelson A. , C. Hussin Marites
Published: April 2023

This study aimed to assess the work engagement and organizational learning capability among educators of DMC College Foundation, Inc. during the second semester of 2022-2023. A quantitative descriptive-correlational research design was used in this study. Data were obtained from 47 educators of DMC College Foundation, Inc. Frequency count, Weighted Mean and Pearson Correlation was used to analyze the gathered data. The study found that when a teacher is tenured, he/she is more engaged in the organization. Among the indicators of work engagement, dedication has the highest mean which means that educators are very much dedicated on the job they are doing. In terms of organizational learning, educators are more immersed with the system of the school having dialogue as the indicator garnering the highest weighted mean. This shows that the school has a well-established protocol in relation to communicating members of the organization. Moreover, this study found out that there is a significant correlation between work engagement and organizational learning capability across age groups anyhow having a positive correlation, age is not a factor that will affect both work engagement and organizational learning capability. Also, the study concluded that there is no significant correlation between work engagement and organizational learning capability when grouped according to years in service. Additionally, work engagement and organizational capability shows a significant and strong positive correlation with each other.
Autoabasto en la dieta campesina del Totonacapan poblano (México): implicaciones para la agrodiversidad
Authors: Espinoza-Pérez José , Cortina-Villar Sergio , Perales Hugo , Soto-Pinto Lorena , Méndez-Flores Orquidia G.
Published: April 2023

Objetivo: caracterizar el patrón alimentario de las familias campesinas y determinar la influencia de los factores socioeconómicos en el origen y consumo de los alimentos en el Totonacapan poblano (México). Metodología: con la técnica de recordatorio de 24 horas, se aplicó una encuesta a 270 familias sobre su dieta y se documentó la información socioeconómica. Resultados: se registraron 159 alimentos, de los cuales 57.6% tiene procedencia externa e incluyen grasas, lácteos, huevo, pan, pasta de trigo, sal y alimentos y bebidas altas en azúcares. El maíz, algunos frijoles y chiles, las hojas verdes “quelites”, las frutas, la carne de pollo y el puerco proceden en gran medida de la producción propia, local y regional. Valor: se aportan datos sobre la dieta campesina actual y sus implicaciones para la agrodiversidad y la salud. Conclusiones: la dieta campesina se está desligando de la producción de autoconsumo debido a la migración asociada con bajos ingresos.