bluuebunny/crossref_metadata_2025 huggingface dataset:
THE RHEOLOGICAL PROPERTIES OF BLOOD IN THE MOST ACUTE STAGE OF ISCHEMIC STROKE AND THEIR RELATION TO THE SEVERITY OF NEUROLOGICAL IMPAIRMENT
Authors: Azhermacheva M. N. , Plotnikov D. M. , Aliev O. I. , Alifirova V. M. , Plotnikov M. B. , Burkova K. I.
Published: June 2020

The study evaluated the rheological parameters of blood: blood viscosity, plasma viscosity, hematocrit, red blood cell aggregation and deformability. The severity of the patients was assessed by clinical scales:Glasgowcoma scale, the scale NIHSS, Barthel index. The study found that in the acute phase of ischemic stroke increased blood viscosity by increasing red blood cell aggregation and reduced erythrocyte deformability. The increase in the viscosity of the blood in acute ischemic stroke is accompanied by increased severity of neurological disorders.
THERAPY EFFECTS AND SAFETY OF RHODIOLA ROSEA EXTRACT WS 1375 IN SUBJECTS WITH SYMPTOMS OF CHRONIC FATIGUE
Authors: Lekomtseva Y. V. , Zhukova I. A. , Tartakovsky I. A.
Published: June 2020

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PROTEINS, PEPTIDES AND AMINO ACIDS AS MARKERS OF BRONCHOPULMONARY DISEASES
Authors: Fyodorov V. I. , Karapuzikov A. A. , Starikova M. K.
Published: June 2020

The article is a review of current literature on a content of proteins, peptides and amino acids in human exhaled breath. The results of proteomics and metabolomics applying for selective detection of individual proteins, peptides and amino acids are described. The study of exhaled breath condensate and exhaled endogenous particles contained lung proteins are considered. The peculiarities of protein, peptide and amino acid content in exhaled breath at various respiratory diseases are described. It is shown that the detectable substances may be specific markers of individual diseases.
DAILY PROFILE PECULIARITIES OF BLOOD PRESSURE IN PATIENTS WITH SENILISM IN THE FRAME OF METABOLIC SYNDROME
Authors: Chernysheva Ye. N. , Panova T. N. , Abdrashitova A. T.
Published: June 2020

Imperfect decrease of systolic and diastolic pressure at night is observed according to 24-hour blood pres-sure monitoring at patients with metabolic syndrome and senilism. According to average daily pulse arte-rial pressure 55.0 (51.0; 60.0) mm. of mercury column the patients with senilism are referred to the risk group to appear cardiovascular complications
SCINTIGRAPHY WITH 123I-METAIODBENZILGUANIDINE IN EVALUATION OF SYMPATHETIC INNERVATION OF THE LEFT VENTRICULAR IN PATIENTS WITH CORONARY HEART DISEASE AND ATRIAL FIBRILLATION
Authors: Lishmanov Yu. B. , Minin S. M. , Yefimova I. Yu. , Saushkina Yu. V. , Kisteneva I. V.
Published: June 2020

The aim of this study was assessment of cardiac sympathetic activity in patients with atrial fibrillation by planar scintigraphy with 123I-metaIodbenzilguanidine (123I-MIBG).Material and Methods. The study included 28 (average age (57.4 ± 11.6) years) patients with suspected coronary artery disease. All patients to divide 3 groups: 13 patients (group 1) with paroxysmal AF (PAF),6 patients (group 2) with long-standing persistent AF (LSPAF) and 10 patients with no signs of AF (group 3).Planar imaging was performed to study initial (imaging started 20 minutes after MIBG injection 148 MBq 123I-MIBG) and delayed (imaging started 4 hours after 123I-MIBG injection). For the anterior planar 123I-MIBG images, regions of interest were constructed for the heart and upper mediastinum, and the heart-mediastinal ratio (HMR).Results. The delayed HMR in patients with PAF and LSPAF was significantly lower compared that in patients third group (1.59 ± 0.16, 1.54 ± 0.17 and 1.82 ± 0.11 respectively, p < 0.05). The washout rate of 123I-MIBG in patients with PAF and LSPAF was significantly highest compared that in patients with no signs of AF (33.4 ± 17.5, 29 ± 11.7 and 17.2 ± 9.6 respectively, p < 0.05). In assessment of regional sympathetic activity in patients with LSPAF defect accumulation 123I-MIBG in both the early and delayed scintigrams was significantly higher compared with groups of patients with PAF and without AF.Results of this study indicated, that the patients with atrial fibrillation has of regional LV myocardial changes, according with 123I-MIBG imaging. More sympathetic innervation abnormality was observed in patients with long-standing persistent AF.
STUDY CARDIOTOXIC PROPERTIES OF NATIVE PEAT HUMIC ACIDS
Authors: Belousov M. V. , Akhmedzhanov R. R. , Zykova M. V. , Maslov L. N. , Guriyev A. M. , Yusubov M. S.
Published: June 2020

The earlier pathomorphological  studies of toxic properties of peat humic acids (PHAs) at intraperitoneal injection revealed that one of the causes of lethality is the direct cardiotoxic action of PHAs. For the further study of the mechanism of cardiotoxic action, electrocardiogram was recorded and ventricular fibrillation threshold was determined in male rats after intraperitoneal injection of humic acids in knowingly lethal dose – 480 mg/kg bwt. A single  intraperitoneal injection  of PHA to rats in knowingly lethal dose caused a significant decrease of the ventricular fibrillation threshold in comparison with intact animals. It was found that the PHA injection exerted no significant effect on the heart rate in rats and change of the PQ, QT, and QTc intervals.  At the same time, the significant shortening of the QRS complex in comparison with intact animals was observed. Judging from the results obtained, the single injection of humic acids in the lethal dose induced a decrease in the electrical stability of heart, as evidenced by the drop of the ventricular fibrillation threshold. This decrease of the electrical stability of heart can be a cause of sudden cardiac death of trial rats. Disorder of the heart electrical stability is likely connected with the ionophoric action of humic acids and change of electrophysiological properties of cell membranes.
ВОЗМОЖНОСТИ РЕНТГЕНОВСКОЙ МАММОГРАФИИ "МАЛЫХ" ФОРМ РАКА МОЛОЧНОЙ ЖЕЛЕЗЫ НА ФОНЕ ФИБРОЗНО-КИСТОЗНОЙ БОЛЕЗНИ
Authors: Bukharin D. G. , Velichko S. A. , Frolova I. G.
Published: June 2020

The analysis of radiologic signs of small size breast cancer in patients with fibrocystic disease has been carried out.The conventional approach to the analysis of mammograms of these patients based on the detection of primary, secondary and indirect cancer symptoms has been found to have insufficient effect.The relationship between mammographic findings and primary tumor size, character and concomitant disease intensity has been proved.The data obtained allow us to arrange the diagnostic process and management of patients with breast lesions.
PROGNOSIS OF FEATURES OF COURSE OF TYPE 2 DIABETES MELLITUS IN PATIENTS WITH NON-PSYCHOTIC MENTAL DISORDERS
Authors: Garganeyeva N. P. , Belokrylova M. F.
Published: June 2020

The logistic regression method is justified prognostic importance of psychosocial factors, along with other factors (predictors), determining the formation of psychosomatic relations and peculiarities of the course of type 2 diabetes mellitus in 203 patients with neurotic, affective and organic non-psychotic disorders, to develop a comprehensive programme for secondary prevention and rehabilitation. Group of high risk of development of DM is constituted by patients with experience of psychosocial stress, whose heightened level of glycaemia is associated with depressive and anxiety disorders.
THE ROLE OF CARBON MONOXIDE IN THE REGULATION OF ELECTRICAL AND CONTRACTILE PROPERTIES OF SMOOTH MUSCLE CELLS OF THE GUINEA PIG URETER
Authors: Kovalyov I. V. , Baskakov M. B. , Gusakova S. V. , Idamzhapova T. A. , Birulina Yu. G. , Rozhkova O. S. , Marchenko A. S. , Smagly L. V. , Medvedev M. A. , Orlov S. N.
Published: June 2020

Carbon monoxide CO, as well as nitric oxide and hydrogen sulfide, make up the family of labile biological mediators termed gasotransmitters. We hypothesized that CO may be involved in the mechanisms of regulation electrical and contractile properties of smooth muscles.The effects of carbon monoxide donor CORM II (tricarbonyldichlororuthenium(II)-dimer) on the electrical and contractile activities of smooth muscles of the guinea pig ureter were studied by the method of the double sucrose bridge. This method allows to register simultaneously the parameters of the action potential (AP) and the contraction of smooth muscle cells (SMCs), caused by an electrical stimulus.CORM II in a concentration of 10 mmol has reduced the amplitude of contractions SMCs to (86.5 ± 9.7)% (n = 6, p < 0.05), the amplitude of the AP to (88.9 ± 4.2)% (n = 6, p < 0.05) and the duration of the plateau of the AP to (91.7 ± 6.0)% (n = 6, p < 0.05). On the background of the action of biologically active substances (phenylephrine, 10 µmol or histamine, 10 µmol), these effects of CORM II amplified. The inhibitory action of СORM II on the parameters of the contractile and electrical activities of the smooth muscles of guinea pig ureter has been decreased by blocking potassium channels in membrane of SMCs by tetraethylammonium chloride (TEA) оr inhibition of soluble guanylate cyclase (ODQ [1H-[1,2,4]-oxadiazolo[4,3-a]quinoxalin-l-one]). On the background of TEA (5 mmol), a donor of CO (10 mmol) caused a reduction the amplitude of contraction SMCs to (87.0 ± 10.8)% (n = 6, p < 0.05), the amplitude of the AP to (91.7 ± 6.4)% (n = 6, p < 0.05) and the duration of the plateau of the AP to (93.4 ± 7.5)% (n = 6, p < 0.05). After the pretreatment of ODQ (1 µmol) adding CORM II (10 mmol) in solution has resulted to augment of the amplitude of contraction ureteral smooth muscle strips to (90.9 ± 4.2)% (n = 6, p < 0.05), the amplitude of the AP to (97.2 ± 10.3)% (n = 6, p < 0.05) and the duration of the plateau of the AP to and (99.7 ± 10.0)% (n = 6, p < 0.05).Thus, can be argued the inhibitory effect of carbon monoxide on the electrical and contractile activities of the guinea pig ureter SMCs is due to changes in the ionic conductivity of the membranes, above all with increasing the potassium conductance or activation of soluble guanylate cyclase.
CONTEMPORARY ISSUES AND PERSPECTIVES ON THE STUDYING OF SCHIZOPHRENIA WITH NEGATIVE SYMPTOMS LEADING
Authors: Kornetova Ye. G. , Semke A. V.
Published: June 2020

Basic contemporary trends in schizophrenia studies with leading negative symptoms in the clinical picture has been presented. There were mainly overviewed the works resulted from original research as well as the papers clinical dynamics, the study of constitutional factors, adjustment as well as schizophrenia therapy with predominant negative symptoms. There was shown interdisciplinary character of the discussed problem in the plane of a biopsychosocial approach. There was demonstrated the priority of updating of clinical patterns phenomenology and schizophrenia dynamics with predominance of negative symptoms in connection with the upcoming transition to the new revision of the international classification of diseases and the need for profound studies of the influence of constitutional factors on the clinical dynamics and prognosis of the disorder in terms of patients adjustment and therapy.
MORPHOLOGICAL CHANGES IN THE HIPPOCAMPUS OF RATS IN ACCELERATED AGING
Authors: Maksimova K. Yu. , Stefanova N. A. , Logvinov S. V.
Published: June 2020

The aim of this work was the analysis of structural changes with age in the hippocampus of senescenceaccelerated OXYS rats when signs of accelerated brain aging are missing (age 14 days), developments (age 5 months), and active progresses (age 15 months). The study was performed on 15 OXYS rats and 15 Wistar rats (as a control). After dislocation, brains were dissected, fixed with 10% formalin, embedded in paraffin, and serially cut in coronal sections (5μm thickness). These sections were stained with Cresyl violet and examined with a photomicroscope (Carl Zeiss Axiostar plus, Germany). The total number of hippocampal pyramidal cells in the CA1, CA3 and the dentate gyrus regions were estimated in 14-dayold, 5and 15-month-old OXYS and Wistar rats (n = 5) on the 5 slices of each brain sections. The number of neurons with chromatolysis, hyperchromatic with darkly stained cytoplasm and shrunken neurons were calculated as degenerative neurons. The pictures obtained with the program Carl Zeiss Axio Vision 8.0 with increasing 10  100, determined the average area bodies and nuclei of neurons (mkm2). The significant structural changes of neurons in the CA1, CA3 and dentate gyrus regions of the hippocampus in OXYS rats at 5 month of age are revealed by light microscopy. This results indicates the early develop neurodegeneration in OXYS rats. The most pronounced morphological changes occur in the CA1 region of the hippocampus of OXYS rats and irreversible. The degenerative changes of neurons in the hippocampus increases by the age of 15 months. Morphometric analysis of the average area of bodies and the nuclei of hippocampal neurons in CA1, CA3 and the dentate gyrus regions of OXYS and Wistar rats at 14 days of age showed no significant interline differences. At 5 months of age in the CA1 region of the hippocampus of OXYS rats was determined a significantly lower average body size and nuclei of pyramidal neurons compared with Wistar rats. With age, these changes have progressed and 15-month-old OXYS rats have a significant decrease in the area bodies and nuclei in all studied regions of the hippocampus compared with Wistar rats. These results confirm the promising use OXYS rats as a model of accelerated brain.
EFFECTS OF GROSSHEMIN AND GROSSMISIN ON THE ACUTE HYPERLIPIDEMIA MODEL INDUCED BY ETHANOL
Authors: Ratkin A. V. , Kaidash O. A. , Ivanov V. V. , Vengerovsky A. I. , Adekenov S. M. , Chuchalin V. S.
Published: June 2020

Objective: study sesquiterpene lactones grosshemin and grossmisin lipid-lowering properties on the model of acute hyperlipidemia induced by ethanol in rats.Materials and methods. Rats during 7 days injected into the stomach grosshemin and grossmisin in a dose 10 mg/kg or reference drug nicotinic acid in a dose 25 mg/kg. Hyperlipidemia caused by single introduction of ethanol into the stomach in a dose 5 g/kg. In blood serum of tail vein measured the triacylglycerols, total cholesterol, high density and low density lipoproteins cholesterol, also the level of free fatty acids. Calculated the ratio of high density lipoproteins cholesterol to the amount of low density lipoproteins cholesterol and the index of atherogenicity.Results. A single dose of ethanol increased serum level of triacylglycerols in 1.9 times, free fatty acids – in 3.2 times, low density lipoproteins – on 44% in comparison with the intact animals indices. It shows the development of acute hyperlipidemia. Serum total cholesterol, high density lipoproteins cholesterol and the index of atherogenicity were not changed. Course sesquiterpene lactones grosshemin and grossmisin introduction against the background of acute hyperlipidemia was accompanied by a decrease in the serum of triacylglycerols levels respectively by 19.8% and 34.1%. Nicotinic acid lowered the content of triacylglycerols by 42.4%. Grosshemin and nicotinic acid reduced the increased level of free fatty acids in the blood serum by 60.7–67.9%. Grossmisin and nicotinic acid decreased by 14.6–17.2% of total cholesterol in the blood serum. In acute hyperlipidemia grosshemin and grossmisin reduced low density lipoproteins cholesterol by 17.6% and 20%, respectively, nicotinic acid – by 15.7%. Both of sesquiterpene lactone and nicotinic acid did not modify the content of high density lipoproteins cholesterol. When introduction grosshemin, grossmisin and nicotinic acid ratio of high density lipoproteins cholesterol to the amount of low density lipoproteins cholesterol significantly increased by 42.8%, 38,6% and 22.1% respectively.Conclusion. Sesquiterpene lactones grosshemin and grossmisin posses hypolipidemic effect in acute experimental hyperlipidemia caused by the ethanol introduction. Lactones normalize many indices of lipid metabolism, which can be caused by different biochemical targets of these molecules. Lactones, as nicotinic acid, in the model of acute hyperlipidemia decrease in blood serum triacylglycerols, total cholesterol, and low density lipoproteins cholesterol content. Grosshemin and nicotinic acid also reduce the free fatty acids level.
CHARACTERISTICS OF TREATMENT OF PATIENTS WITH ACUTE AND CHRONIC LARYNGITIS DEPENDING ON RESULTS OF BACTERIOLOGICAL EXAMINATION OF LARYNX MICROFLORA
Authors: Chuikova K. I. , Zaripova T. N. , Mukhina V. I.
Published: June 2020

This paper describes study of larynx microflora in patients with acute laryngtis and exacerbations of chronic laryngitis. Therapeutic algorithm based on bacteriologic examination data was developed reduce. New algorithm helps reduce sickness terms and to decrease prevalence of desease.123 patients of age from 18 to 60 were under observation: 43 patients with acute laryngitis and 80 patients with exacerbation of chronic laryngitis. 22 patients with acute laryngitis and 58 patients with exacerbation of chronic laryngitis underwent etiotropic treatment with antibiotics. The group of comparison with acute laryngitis (21 patients) and chronic laryngitis (22 patients) received treatment according to the conventional scheme.Bacteriologic examination of larynx mucous, clinical study and functional voice test (time of maximum vowels phonation) before and after treatment were carried out.As a result of the research it was established that the most common causative agent of acute and chronic inflammatory larynx diseases is S. aureus as a mono culture or combined with other bacterial associations (S. аnhemolyticus, Str. viridans etc.).After the end of antibacterial therapy we found symptoms as hoarseness, irritation, dry laryngopharynx, hyperemia and swelling of larynx mucous reduced eather in new treatment group than controlled group. Time of maximum vowels phonation (in seconds) also increased significantly.Sickness terms after prescription of new treatment was shorter than in comparison groups: (10.9 ± 7.9) days for acute laryngitis and (12.6 ± 7.3) days for chronic laryngitis respectively. Health index was 20.8 and 19.5% respectively.
CARDIOVASCULAR MAGNETIC RESONANCE WITH CONTRAST AGENT: PROGNOSTIC ROLE IN DETERMINING ARRHYTMOGENIC FOCUS
Authors: Bogunetsky A. A. , Ussov V. Yu. , Babokin V. Yu.
Published: June 2020

In order to evaluate the possibilities of MRI in detection of proarrhytmogenic areas of myocardium we did compare directly and quantitatively the local myocardial uptake of paramagnetics to the results of electrophysiologic study of myocardium in twenty-two patients with recent transmural myocardial infarction and frequent ventricular extrasystoles verified by ECG studies.Topic quantitative study of myocardial electric potential has been carried out using Carto XP system (by Biosense Webster).By analysis of the images the measurement of Index of transmurality (IT) for the contrast uptake was calculated as ratio of thickness of paramagnetic uptake to overall thickness of myocardium in a specific segment.The the electrophysiologic and MRI data were analyzed topically as segment vs segment and the significant tendency was revealed with simultaneous grow of pro-arrhythmogenic activity and value of IT. In other words, as bigger the IT increases, as arrhythmogenic the lectrical activity becomes. The best border value of IT that provided separation of electrically normal segments from proarrhythmogenic ones was the IT = 0.3.Therefore we conclude the contrast-enhanced MRI of myocardium deliver additional information on risk of arrhythmias, not only the detection of myocardial damage itself.
ROLE OF NEUROSPECIFIC PROTEINS IN THE DEVELOPMENT OF COGNITIVE DYSFUNCTION IN PATIENTS WITH TYPE 1 DIABETES
Authors: Novosyolova M. V. , Samoilova Yu. G. , Zhukova N. G. , Latypova A. V.
Published: June 2020

Type 1 (type 1 DM) diabetes mellitus is one of the common chronic metabolic diseases, which currently is a significant problem due to disability at a young age and reduce life expectancy. Despite the fact that type 1 diabetes accounts for only 10% of all patients with diabetes, it occurs particularly hard, with a tendency to progression. One of the targets of type 1 diabetes is the central nervous system with the further formation of cognitive dysfunction in young age leads to diminished quality of life. Cognitive deficits may be the result not only of structural lesions of the brain, but it may be due to the development of metabolic disorders. In the case of timely diagnosis and treatment of cognitive impairment associated with metabolic changes that can partially or completely regress. The aim of this study was to identify biomarkers of the brain damage in young patients with type 1 diabetes. The study involved 58 patients with  type  1  diabetes,  the  control  group  comprised  29  healthy  controls.  The  complex  included a neuropsychological examination which was used for testing the Montreal scale (MoCA test) rapid screening of cognitive impairment, assessment of quality of life using a common questionnaire Medical Outcomes Study Short Form (MOS SF-36) and the specific audit – dependent quality of life (ADDQoL). To evaluateearly markersin the developmentof cognitive dysfunctionwere identifiedneurospecific proteins – S100 protein and glial fibrillary acidic protein (GFAP), myelin basic protein (MBP). Found an increased level of neurospecific protein that was correlated with parameters of carbohydrate metabolism, poor quality of life and severe cognitive deficiency (MoCA test lower than 26 points).
BILE LITHOGENICITY IN PATIENTS WITH TYPE 2 DIABETES AND ITS CORRECTION
Authors: Pavlenko O. A. , Babich Ye. N.
Published: June 2020

We conducted a study to assess the bile lithogenicity in patients with type 2 diabetes mellitus (DM). We have found increased levels of cholesterol both in the gallbladder and hepatic bile. The study showed a decrease of cholatocholesterol coefficient (HHC), increase of lithogenic index Thomas–Hofmann and Rubens in all patients with type 2 diabetes, particularly in the gallbladder bile, indicating the colloidal destabilization of bile and an increased tendency to stone formation. Especially pronounced lithogenicity bile in patients with type 2 diabetes was associated with age in patients over 40 years of age and concomitant  overweight  or  obese  (BMI > 27 kg/m2).  After  a  course  of  ursodeoxycholic  acid  (UDCA)  at a dose of 15 mg/kg of body weight per day for 2.5–3 months was reduced lithogenicity gallbladder bile in patients with type 2 diabetes mellitus when the duration of disease was up to 5 years in 1.3 times, more than 5 years – in 1.5 times, more than 10 years – 1.7 times according to the index Thomas–Hofmann and Rubens.
WAIST CIRCUMFERENCE AS INDICATOR COMPONENTS OF METABOLIC SYNDROME IN THE SIBERIAN POPULATION
Authors: Simonova G. I. , Mustafina S. V. , Nikitin Yu. P. , Shcherbakova L. V.
Published: June 2020

Object of research: to determine the cut-off point of waist circumference (WC) identify the components of metabolic syndrome (MS) in a large industrial center of Western Siberia.In the period from 2003 to 2005 in the screening population surveyed 9362 people, including 4268 men (45.6%) and 5094 women (54.4%).The response rate was 61%. The study protocol included a sociodemographic data, measurement of blood pressure (BP), anthropometry (height, weight, WC), the definition of biochemical parameters. Statistical analysis was performed using the package SPSS. To determine the cut-off points of the used ROC model.In the group with ≥2 components of MS cut-off point WC from the men was 93.3 cm (sensitivity 72%, specificity 83%), women – 90.2 cm (sensitivity 72%, specificity 62%).The lowest cut-off point WC marked BP ≥ 130/85 mm Hg for men – 90 cm (sensitivity 66.8%, specificity 58.1%), for women – 86.5 cm (sensitivity 71.0%, specificity 55.7%). The highest cut-off point from the defined patients with diabetes for men was 99.0 cm (sensitivity 61%, specificity 70.2%), for women – cm (sensitivity 64.1%, specificity 65.9%).Conclusions: Cut-off point from the persons with the presence of ≥2 MS components in Siberian population 45–69 years, in accordance with the ROC analysis are 93.3 cm for men and 90.2 cm for women. The lowest cut-off point WC marked BP ≥ 130/85 mm Hg for men – 90 cm, for women – 86.5 cm. The highest cut-off point from the defined patients with diabetes for men was 99.0 cm, for women – 95 cm.
SOURCES OF THE ELECTROPHYSIOLOGY AND ELECTROCARDIOGRAPHY
Authors: Tetenev F. F. , Stepanischeva A. N.
Published: June 2020

The basic stages of development of electro-physiology and electrocardiography, 17 portraits and years of life of scientists, developing the fundamental aspects of electro-physiology, and also applied direction clinical electrocardiography, are presented in the article. By the most large scientists, developing fundamental    direction    in    electro-physiology    were    P. Arrhenius,    V.Yu. Chagovec,    Yu. Bernshteyn, A. Khodzhkin and E. Khaksli. In development of the applied direction in electrocardiography the role is great of V. Eyntkhoven, A.V. Samoilov and N. Holter. In period of formation of formation of electrophysiology, electrocardiography and at present scientists in ours country preferently working in applied direction.
DEVELOPMENT OF THE TECHNIQUE OF QUANTITATIVE DEFINITION OF TRIAZAVIRIN IN WATER SOLUTIONS WITH USE OF THE SPECTROPHOTOMETRY METHOD
Authors: Kinev M. Yu. , Melnikova O. A. , Petro A. Yu. , Zaboyarkina D. V.
Published: June 2020

Authors of article developed techniques of quantitative definition Triazavirin in water solutions with use of a method of a spectrophotometry. Three options of a method of a s spectrophotometry are used: direct spectrophotometry, spectrophotometry according to A.M. Firordt, a spectrophotometry with use of standard solution. For all techniques calculation of metrological characteristics is carried out. The developed techniques of quantitative definition were applicable for practical use in the pharmaceutical analysis. The relative error of quantitative definition Triazavirin in water solutions made 0.37%, 0.14% and 8.29% respectively for a direct spectrophotometry, a spectrophotometry with use of standard solution, a spectrophotometry according to A.M. Firordt.
MULTILOCI SEQUESTERANT STRAINS OF STREPTOCOCCUS PNEUMONIAE ISOLATED FROM ELDERLY PATIENTS WITH COMMUNITY ACQUIRED PNEUMONIA
Authors: Martynova A. V. , Balabanova L. A. , Chulakova O. A.
Published: June 2020

Comuunity-acquired pneumonias in aged patients is the significant epidemiology problem for the public health of almost all the countries. Even more important the problem of microbiological monitoring and epidemiology surveillance for the S. pneumoniae strains as one of the ubiquitous pathogens causing as the community-acquired pneumonias as well the other infections of respiratory tract, what defines their different epidemiological meaning.Multilocus sequence typing is the perspective method of molecular epidemiological surveillance allowing to define the epidemiologically dangerous clones of the ubiquitous microorganisms as Streptococcus pneumomiae. The aim of our research was to conduct the multilocus sequence typing of pneumococci strains isolated in patients with community acquired pneumonias, bronchitis in aged patients.Materials and methods. There were taken 14 strains of S. pneumoniae, isolated in patients with community-acquired pneumonias (seven of them were multiresistant), eight strains were isolated from patients with the chronical onstructive lung diseases and four strains from carriers. Multilocus sequence typing was conduected according to method to M.C. Enright and B.G. Spratt (1998).Results. The strains, isolated in all populations were the related isolates of the species S. pneumoniae, the most of them had the unique genotype defining the sequence type for every strain. There were 6 strains of Taiwan 19F-14 genotype from 14 strains isolated in aged patients with community-acquired pneumonia. Among strains isolated from carriers there were prevailing the strai of R6 genotype.Conclusion. Multilocus sequence typing allows to identify the new genotypes and to prognose the appearing of epidemiologically dangerous strains with new peculiarities.
INFLUENCE OF THE DIHYDROQUERCETIN AND ARABINOGALACTAN COMPOSITION ON LYMPH FLOW
Authors: Shamanaev A. Yu. , Ivanov I. S. , Sidekhmenova A. V. , Plotnikov M. B.
Published: June 2020

Purpose. To evaluate lymphokinetic activity of the dihydroquercetin and arabinogalactan composition.Material and methods. The investigation was carried out on male Wistar rats. Lymphokinetic activity was determined by the rate of lymph outflow through the puncture of cisterna chili after intragastricall administration of the dihydroquercetin and arabinogalactan composition with ratio 1 : 5 (50 mg/kg + 250 mg/kg).Results. It was established that lymphokinetic activity of the dihydroquercetin and arabinogalactan composition with ratio 1 : 5 (50 mg/kg + 250 mg/kg) exceeds activity of it´s components.
HIGH PERFORMANCE INFORMATIONAL ENVIRONMENT FOR CALCULATIONS IN BIOMEDICINE
Authors: Brazovsky K. S. , Pekker Ya. S. , Dyomkin V. P. , Umansky O. S. , Tolmachyov I. V.
Published: June 2020

This work represented one of the possible approaches to providing the required computational resources for solving the complex data processing tasks in biomedicine. The proposed solution is based on four tightly interacted key components of modern high performance computational systems: high perfor­mance computational cluster, data processing center, specialized data store and protected telecommunication channels. The examples of tasks, which have been solved in the developed information environment, are given.
APPLICATION OF COMPUTER TECHNOLOGIES IN TEACHING OF MEDICAL STUDENTS
Authors: Vorobeichikova O. V.
Published: June 2020

he purpose of the given research are situational tasks from the point of view of algorithms of their decision and application of computer technologies for realization of similar algorithms. In the beginning the concept of a situational task and an opportunity of their use for training medical students is considered. The analysis of existing situational clinical tasks is spent and classification of algorithms of the decision is resulted. The opportunity of application of computer technologies for realization of similar algorithms is considered. Among all existing algorithms of the decision one in which the algorithm can be applied to the decision of the same tasks of one class is especially allocated. The technology of construction of such algorithm is resulted and the description of a program complex which realizes such algorithm of the decision of situational tasks is given.
MEDICAL CYBERNETICS AND INTERDISCIPLINARY TRAINING OF SPECIALISTS FOR MEDICINE
Authors: Pekker Ya. S. , Novikova T. V.
Published: June 2020

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POLYMORPHISM OF GENES OF IMMUNOSUPRESSIVE CYTOKINE IL-10 AND TGF-β AT TUBERCULOSIS INFECTION
Authors: Churina Ye. G. , Urazova O. I. , Novitsky V. V. , Filinyuk O. V.
Published: June 2020

The aim of the work was the study of connection of allelic polymorphism of IL10 and TGFВgenes with changes in the basal and BCG-induced production of immunosuppressive cytokines IL-10 and TGF-β by mononuclear leukocytes in vitro in patients with the first diagnosed pulmonary tuberculosis (TB), depending on the clinical form of the disease. The evaluation of the cytokines production was conducted by measuring its concentration in culture supernatants by ELISA. The allele-specific amplification of specific stretches of the genome was used for the study of polymorphic genes of cytokines. The DNA and supernatants of culture suspensions of blood mononuclear leucocytes in healthy volunteers and patients with TB were the material of the research. It was shown in the research conducted that the basal and BCG-induced over-production of IL-10 in vitro occurs in patients with TB, regardless of the genotype of the locus of C-592AIL10 gene. In addition, genotype AA of polymorphism of IL10gene in patients with infiltrative and disseminated TB is associated with the maximum production of IL-10 in vitroand genotype CC – with the minimum production of this cytokine in vitro. Analysis of the production of TGF-β in vitro in patients with TB showed its increase only in case of carriage of allele T (C-509T) of TGFB gene. In patients with disseminated TB and homosygotic genotype TT the increase in both basal and BCG-induced production of TGF-β was determined, and in patients with infiltrative TB – only after induction of cells by BCG-antigen.

Thus, the over-production of cytokines with inhibiting activity in patients with TB is genetically determined and promotes the formation of suppressive mode of immune-regulation. The increase in the secretion of cytokines IL-10 and TGF-β in vitro in patients with TB are associated with carriage of allele A and genotype AA (C-592A) of IL10gene and allele T and genotype TT (C-509T) of TGFB gene.
DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b
Authors: Shitikova O. G. , Sherstoboev E. Yu. , Masnaya N. V. , Danilets M. G. , Trofimova E. S. , Ligacheva A. A. , Madonov P. G. , Kinsht D. N. , Ershov K. I. , Shilova M. A.
Published: June 2020

The development of optimal experimental therapeutic schemes of immobilized interferon α-2b (production of JSC «Siberian Center for Pharmacology and Biotechnology», Novosibirsk) administration was conducted in the frame of the common program of pre-clinical study. The drug being studied was administered intragastrically for 3, 5 and 7 days in the therapeutic dose (1.8 × 105 IU/kg).  Experimental evaluation of immunotropic properties of immobilized interferon α-2b was carried out at in vivoexperiments in outbred CD1 male mice with using of following tests: the study of drug effect on the phagocytic activity of peritoneal leukocytes (the relative number of phagocytes and the number of bacteria which were assimilated by phagocytes were assessed); the study of drug effect on the humoral immune response (the total number of splenocytes, the relative and absolute number of antibody-forming cells and their functional activity were determined); estimation of drug effect on the cellular immune response with the using of delayed-type hypersensitivity test induced by sheep red blood cells. Based on these data we concluded that course administration of immobilized interferon α-2b in the therapeutic dose for 5 days increased phagocytosis of neutrophils and humoral immune response most effectively. Cell-mediated immune response was significantly increased after 7-fold administration of studied drug.
PERSONALIZED MEDICAL WEB SERVICES IN THE HEALTH CARE SYSTEM OF THE RUSSIAN FEDERATION
Authors: Maksimov D. Ye. , Mishkina A. I. , Karas S. I. , Finchenko Ye. A.
Published: June 2020

Personal medical records of the patient in electronic form are the basis of modern health care information. With the development of medical technologies for patients have more opportunities to independently monitor various indicators of health. Currently, the process of self-observation of the patient is not possible without the use of modern medical equipment, allowing to measure the vital parameters of the body.

It is important to storage and processing of information on the measured patient or family health indicators in a single system. This function is implemented in the Web services introspection of patient. Review of the most popular implementations of Web services listed in this article.
DEFINITION AND ANALYSIS OF MOTION ACTIVITY AFTER-STROKE PATIENT FROM THE VIDEO STREAM
Authors: Katayev M. Yu. , Katayeva N. G. , Katayev S. G. , Abramov M. O. , Chistyakova V. A.
Published: June 2020

This article describes an approach to the assessment of motion activity of man in after-stroke period, allowing the doctor to get new information to give a more informed recommendations on rehabilitation treatment than in traditional approaches. Consider description of the hardware-software complex for determination and analysis of motion activity after-stroke patient for the video stream. The article provides a description of the complex, its algorithmic filling and the results of the work on the example of processing of the actual data. The algorithms and technology to significantly accelerate the gait analysis and improve the quality of diagnostics post-stroke patients.
LONG-TERM RESULTS OF PROCTECTOMY WITH THE RECONSTRUCTION OF THE SPHINCTER, LEVATOR AND RESERVES-STORAGE TANK WITH LOW RECTAL CANCER
Authors: Bashirov S. R. , Trifonov M. N. , Tikhonov V. I. , Grishchenko M. Yu.
Published: June 2020

Proctectomy with total mesorectal excision, resection of the internal sphincter, deep portions of the external sphincter and reductionwas performed on nine patients aged from 38 to 74 years with adenocarcinoma of low rectal cancer (T2N0M0 (n = 4), T2N1M0 (n = 1), T3N0M1 (n = 3), T4N2M1 (n = 1)), located in 8–25 mm from an anus. Simultaneously, atypical resections of the liver were performed on two of the patients. In the perineal section of the reduced intestine a sphincter was created from a smooth muscle cuff, a levator from not free serous, and a muscular flap, fixed to a forward wall of the small basin, and a reservoir of cross stitched submucosa site formed after the formation of a levator.  5 of the patients (T2N0-1M0)had no disease advance 3–9 years. They were able to hold firm feces, but were less able to hold liquid feces, and sometimes didn’t hold intestinal gas or differentiate between feces and gas, but were capable of ignoring an urge to defecate and to detain a defecation (level of a fecal continence on a scale of "Wexner score" (11 ± 1) of points).
COMMUNICATION OF NAD- AND NADP-DEPENDENT DEHYDROGENASE ACTIVITIES IN LYMPHOCYTES WITH OUTCOME OF PREVALENCE PURULENT PERITONITIS
Authors: Savchenko A. A. , Zdzitovetsky D. E. , Luzan N. A.
Published: June 2020

Aim of this study was to investigate of NAD(P)-dependent dehydrogenases activity in lymphocytes at different outcomes widespread purulent peritonitis(WPP). Enzyme activity in blood lymphocytes was determined by bioluminescent method. Found that patients with a poor outcome of WPP in blood lymphocytes made more pronounced reaction of lipid anabolism and catabolism of xenobiotics. Regardless of the outcome of the disease in blood lymphocytes of WPP patients reduced levels of enzymes activity that determine the intensity of anaerobic and aerobic respiration and the level of plastic processes, but the activation reactions of lipid catabolism and transport products through glycerol-3-phosphate dehydrogenase on reactions of glycolysis. Based on the informativeness of the NAD(P)-dependent dehydrogenases activity in the classification neural network model for the disease outcome, metabolic coefficient is proposed, which has prognostic significance.
CHARACTERISTICS OF LIPID METABOLISM IN PATIENTS WITH TYPE 1 DIABETES, DEPENDING ON THE AVAILABILITY OF DIABETIC MICROANGIOPATHY AND DIET
Authors: Samoilova Yu. G. , Yurchenko Ye. V.
Published: June 2020

Was investigated 67 children (36 boys and 31 girls) in age from 6 to 18 years old, with type 1 diabetes patients who were treated at the Children's Hospital Endocrinology number 1 inTomsk, average age of the boys at the time of the survey amounted to (13.0 ± 0.4) years, girls – (13.5 ± 0.4) years. All children diagnosed with type 1 diabetes were under subcompensation. Clinical evaluation of patients included a medical history, physical examination, evaluation of physical development. A study of lipid profile (high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL), cholesterol (TC), triglycerides (TG), atherogenic index). Screening for diabetic retinopathy was performed by examining the fundus by direct ophthalmoscopy after pupil dilation. Diagnosis of diabetic nephropathy, retinopathy performed according to the classification. Patients were divided into groups depending on the presence of complications, and the level of compensation lipid metabolism (children with satisfactory compensation of lipid metabolism (total cholesterol level less than 5.2 mmol / l), children with poor compensation lipid metabolism (total cholesterol above 5.2 mmol / l)). Nature of the subjects studied nutrition using  a questionnaire drawn up on the basis of the standard dietary questionnaire program CINDI, allowing to evaluate the eating habits by examining the frequency of intake of various foods, food preferences and priorities. Products, according to the lipid-lowering diet on the recommendations of the European Society for atherosclerosis were combined and divided into groups according to frequency of use: commonly used products, and rarely use the products. Mathematical treatment of the results was performed using the software package Statistica 5.5 for Windows. In the first phase expected to total group performance statistics – the arithmetic mean value M and the error of the mean m, as well as visualization of the distribution of values was performed using frequency histograms. To select a criterion to assess the significance of paired differences check compliance form normal distribution using the criterion χ2, and also controlled the general equality of variances using the F-test Fisher. Given that these conditions are satisfied only part of the empirical distributions of features tested the hypothesis that the general average in all cases was performed using Mann–Whitney U-test for independent variables. Null hypothesis was rejected when p < 0.05.

In the study of lipid metabolism in patients with type 1 diabetes, no increase in the level of cholesterol, triglycerides, HDL, LDL and VLDL – cholesterol in patients with a combination of diabetic nephropathy and retinopathy compared with patients with type 1 diabetes without diabetic microangiopathy. Investigation of the nature of power in patients with type 1 diabetes, allowed us to estimate its impact on lipid metabolism.
IMMUNITY STATE IN THE OFFSPRING OF RATS EXPOSED ANTIGENS TOXOPLASMA GONDII
Authors: Sokolova T. F. , Novikov D. G. , Indutny A. V. , Turok N. Ye. , Velichko G. N.
Published: June 2020

Today the questions about possibility of development disturbances in the immune system of the fetus and the newborn in chronic toxoplasmosis are poorly understood. Aim of research: to detect immunological disturbances in the offspring of rats which have been administered antigens T. gondii.

Two series of experiments was performed. In these experiments white female Wistar rats in the III trimester of pregnancy have been administered corpuscular antigen T. gondii. The 60 days-old offspring of these rats have been included in study group of 137 animals. CD3+ cells count was performed in peripherical blood and standard suspension of splenocytesrats offspring. Peripherical blood cells count was performed in the blood of the rats offspring. In the second experiment rats offspring have been administered sheep erythrocytes in 5 days, before euthanasia. In spleen of this rats antigen-produced cells was counted.In control group was included 118 animals, which was born from white female Wistar rats have been administered 0,9% NaCl solution. CD3+ cells was detected in Cytomics FC500 flow cytometry analyzer (Beckman Coulter,USA) by use rats origne-specifed monoclonal antibodies Anti-Rat CD3-FITC (Beckman Coulter,USA). Hematological parameters was assessed by use hematological analyzer Excell-22 (USA).

We observed, that CD3+ lymphocytes and antigen-produced cells was decreased in test group (degress of decrease CD3+ cells was 17,2%; р = 0,003 in spleen vs. control group, degress of decrease antigen-produced cells was 27,3%; р = 0,03 vs. control group). Number of leukocytes was increased in in test group (34,5%; р = 0,009 vs. control group). Power and strength correlation pleiades between studied blood and spenal markers were higher in in test group vs. control group (∑Gi = 16; ∑Di = 4,38 vs. ∑Gi = 13; ∑Di = 2,28). This phenomenon is probably due to the development adaptive reactions disruption in the immune system and development secondary immunodeficiency states in the offspring of rats.

Violations of immunoreactivity, as well as disruption the possibility of operational restructuring of the immune system and choosing the best immune response in the offspring of rats whose perinatal development take place under the effect of antigens T. gondii, persists for a long time – within 60 days of postnatal life.
PHASE INTERACTION BETWEEN EEG RHYTHMS IN THE STUDY OF PROCESSES OF TIME PERCEPTION
Authors: Bushov Yu. V. , Svetlik M. V.
Published: June 2020

The present study pursued to investigate the role of phase interactions between EEG rhythms in the process of the perception of time. The purpose of the study was to analyse the dependence of these interactions on the type and stage of the activity being performed, as well as on the individual characteristics of a human. For this purpose, 27 boys and 29 girls, all university students, were asked to reproduce and measure short intervals of time (200 and 800 ms), during which their EEG was recorded in frontal, central, parietal, temporal, and occipital lobes, according to the system 10–20%. While studying phase interactions between EEG rhythms, we used wavelet bispectral analysis and calculated the bicoherence function. As it follows from the conducted research, most often close phase interactions are observed between the gamma-rhythm and other rhythms of EEG or between different frequencies of the gamma-rhythm. It was established that the phase interactions under study were influenced by the factors of “sex”, “activity type”, and “activity stage”. The study showed correlations of phase interactions with the levels of intellect, extraversion, neuroticism, with the particularities of the lateral organisation of brain, and the accuracy of time perception.
SODIUM-POTASSIUM-CHLORIDE COTRANSPORT IN THE REGULATION OF VASCULAR MYOGENIC TONE
Authors: Orlov S. N. , Koltsova S. V. , Anfinogenova Ya. D. , Kapilevich L. V. , Gusakova S. N. , Smagliy L. V. , Baskakov M. B. , Medvedev M. A.
Published: June 2020

The article discusses the data on the functioning of Na+,K+,2Cl– cotransport – the carrier providing electroneutral symport of sodium, potassium and chloride, as well as molecular mechanisms of the regulation and physiological significance of this carrier. We analyzed the novel data on involvement of ubiquitous isoform of Na+,K+,2Cl–cotransporter (NKCC1) in regulation of vascular smooth muscle contraction, and role of this carrier in the regulation of cell volume and intracellular chloride concentration.
COGNITIVE DYSFUNCTION IN PATIENTS WITH ISCHEMIC HEART DISEASE
Authors: Yeremina O. V. , Petrova M. M. , Prokopenko S. V. , Isaeva N. V. , Kaskaeva D. S.
Published: June 2020

Coronary artery bypass graft surgery (CABG) is one of the most effective methods of surgical treatment of ischemic heart disease (IHD). However, even improvement of life quality is evident in patients underwent CABG, some neuropsychological, cognitive alterations may appear. Therefore, the problem of neurological complications in cardiac surgery is still of current interest. Prevalence of cognitive dysfunction after CABG varies from 12 to 79%. One third of the patients have alterations of cognitive functions for 1 year and more after the operation. In this review, we will discuss the current understandings on etiology, pathogenesis and prevalence of cognitive dysfunction in patients with IHD after CABG. The following factors determine development of long-lasting cognitive deficiency: lower educational level, advanced age, significant alterations of cognitive functions in preoperative period. At present, age, comorbid pathology (arterial hypertension, diabetes mellitus, vascular diseases, lung diseases), heart fai­lure, alcohol and drug abuse, preoperative cognitive dysfunction are considered as major risk factors for CABG-induced neurological complications. Importance of timely diagnostics and early beginning of treatment of cognitive dysfunction will be discussed.