bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the vertical well seepage model in composite carbonate reservoirs
Authors: Minjing Chen , Zhan Qu , Jiajun Cao , Junhang Hu , Xinggang Meng , Shuaihu Jia , Chenyu Zhang
Published: November 2023

Introduction: Carbonate reservoirs are widely distributed throughout the world. Due to the good physical properties of the reservoirs, they are easily contaminated by mud during drilling opening, so they are often put into production after acidification. After acidification, the near-well reservoir’s physical property is improved, while the far-well reservoir’s physical property remains unchanged. Such reservoirs are commonly called composite reservoirs. Composite carbonate reservoir seepage law is more complicated.

Methods: In this paper, the point source function of a triple-medium composite reservoir is established using the source function theory, Laplace transform, perturbation transform, and linear superposition principle of partial differential equation.

Results: Second, the vertical well seepage model of a composite reservoir is obtained through the new point source function.

Discussion: Finally, the correctness of the model is verified, and the sensitivity analysis of the key parameters affecting the seepage law is carried out. The applicability of the model established in this paper is demonstrated by two wells in the field. This paper provides a theoretical basis for vertical well test analysis of composite reservoirs.
Study on slope stability under excavation and water storage based on physical model tests
Authors: Hou Chengheng
Published: November 2023

Understanding the characteristics and mechanism of slope deformation caused by slope excavation and water storage is very important in the stability analysis of slope engineering. Therefore, based on similarity theory, a physical model test of excavation and water storage was established, and the deformation characteristics of the slope (three-stage excavation and five-stage water storage) were studied by using the monitoring technology of multiple measuring devices. The variation characteristics of the displacement, stress and pore water pressure in the slope were revealed. The results show that the contents of cement and yellow sand can regulate the physical, mechanical and hydrological properties and that gypsum and hydraulic oil have an effect on the permeability. Excavation leads to deformation of the rock mass in the middle and lower slope to the outside of the slope. This is attributed to the stress release, and local stress concentration occurs at the foot of the slope after redistribution. Moreover, the rock mass located in the fault zone shows nonglobal movement because the hanging wall rock mass will produce relative dislocation along the fault zone under self-gravity. Under water storage, the slope body is affected by the hydrostatic pressure, and transient compaction deformation occurs first. As water permeates into the slope body, the water weakens the rock mass, resulting in the gradual deformation of the rock mass near the slope surface to the outside of the slope. The above model test results can provide a valuable reference for slopes stability analysis in construction and subsequent operation.
miR-135a-5p overexpression in peripheral blood-derived exosomes mediates vascular injury in type 2 diabetes patients
Authors: Xie Kangling , Li Cui , Wang Mingzhu , Fu Siqian , Cai Ying
Published: November 2023

Objective

Diabetes pathology relies on exosomes (Exos). This study investigated how peripheral blood Exo-containing microRNAs (miRNAs) cause vascular injury in type 2 diabetes (T2D).

Methods

We removed DEmiRNA from T2D chip data from the GEO database. We isolated Exo from 15 peripheral blood samples from T2D patients and 15 healthy controls and measured Exo DEmiRNA levels. We employed the intersection of Geneards and mirWALK database queries to find T2D peripheral blood mRNA-related chip target genes. Next, we created a STRING database candidate target gene interaction network map. Next, we performed GO and KEGG enrichment analysis on T2D-related potential target genes using the ClusterProfiler R package. Finally, we selected T2D vascular damage core genes and signaling pathways using GSEA and PPI analysis. Finally, we used HEK293 cells for luciferase assays, co-cultured T2D peripheral blood-derived Exo with HVSMC, and detected HVSMC movement alterations.

Results

We found 12 T2D-related DEmiRNAs in GEO. T2D patient-derived peripheral blood Exo exhibited significantly up-regulated miR-135a-3p by qRT-PCR. Next, we projected miR-135a-3p’s downstream target mRNA and screened 715 DEmRNAs to create a regulatory network diagram. DEmRNAs regulated biological enzyme activity and vascular endothelial cells according to GO function and KEGG pathway analysis. ErbB signaling pathway differences stood out. PPI network study demonstrated that DEmRNA ATM genes regulate the ErbB signaling pathway. The luciferase experiment validated miR-135a-3p and ATM target-binding. Co-culture of T2D patient-derived peripheral blood Exo with HVSMC cells increases HVSMC migration, ErbB2, Bcl-2, and VEGF production, and decreases BAX and ATM. However, miR-135a-3p can reverse the production of the aforesaid functional proteins and impair HVSMC cell movement.

Conclusion

T2D patient-derived peripheral blood Exo carrying miR-135a-3p enter HVSMC, possibly targeting and inhibiting ATM, activating the ErbB signaling pathway, promoting abnormal HVSMC proliferation and migration, and aggravating vascular damage.
Case Report: A novel de novo variant of COL1A1 in fetal genetic osteogenesis imperfecta
Authors: Mai Qiuyan , Han Ruining , Chen Yinlong , Shen Ke , Wang Shimin , Zheng Qingliang
Published: November 2023

Objective

Osteogenesis imperfecta (OI) is a rare genetic disorder. Clinical severity is heterogeneous. The purpose of this study was to investigate the genetic characteristics of a fetus with OI by whole exome sequencing (WES) and identify the cause of the disease.

Methods

In this study, a fetus with osteogenic dysplasia was referred to our hospital. DNA was extracted from the aborted fetal tissue and peripheral blood of the parents. To identify the pathogenic genes, we conducted the trio-WES using DNA. A de novo variant in the COL1A1 gene is suspected to be the cause of the OI phenotype. We used Sanger sequencing for validation and various bioinformatics methods (such as SIFT, PolyPhen2, Mutation Taster, conservative analysis, SWISS Model, glycosylation site prediction, and I-Mutant 2.0) for analysis.

Results

Both WES and Sanger sequencing identified a novel de novo variant of COL1A1 (c. 1309G>A, p. Gly437Ser) in a fetus with OI. Bioinformatic analysis showed that the affected residue, p. Gly437, was highly conserved in multiple species and predicted that the variant was deleterious and may have an impact on protein function. This variant is present in highly conserved glycine residues of Gly-X-Y sequence repeats of the triple helical region of the collagen type I α chain, which may be the cause of OI.

Conclusion

This study revealed that the c.1309G>A (p. Gly437Ser) variant in the COL1A1 gene may be the genetic cause of fetal OI in this case. The discovery of this variant enriched the variation spectrum of OI. WES improves the accurate diagnosis of fetal OI, and doctors can provide patients with appropriate genetic counseling.
Genomic mechanisms of plant growth-promoting bacteria in the production of leguminous crops
Authors: Adedayo Afeez Adesina , Babalola Olubukola Oluranti
Published: November 2023

Legumes are highly nutritious in proteins and are good food for humans and animals because of their nutritional values. Plant growth-promoting bacteria (PGPR) are microbes dwelling in the rhizosphere soil of a plant contributing to the healthy status, growth promotion of crops, and preventing the invasion of diseases. Root exudates produced from the leguminous plants’ roots can lure microbes to migrate to the rhizosphere region in other to carry out their potential activities which reveals the symbiotic association of the leguminous plant and the PGPR (rhizobia). To have a better cognition of the PGPR in the rhizosphere of leguminous plants, genomic analyses would be conducted employing various genomic sequences to observe the microbial community and their functions in the soil. Comparative genomic mechanism of plant growth-promoting rhizobacteria (PGPR) was discussed in this review which reveals the activities including plant growth promotion, phosphate solubilization, production of hormones, and plant growth-promoting genes required for plant development. Progress in genomics to improve the collection of genotyping data was revealed in this review. Furthermore, the review also revealed the significance of plant breeding and other analyses involving transcriptomics in bioeconomy promotion. This technological innovation improves abundant yield and nutritional requirements of the crops in unfavorable environmental conditions.
Assessing phytoplankton community composition in the Atlantic Ocean from in situ and satellite observations
Authors: Brotas Vanda , Ferreira Afonso , Veloso Vera , Tracana Andreia , Guerreiro Catarina V. , Tarran Glen Adam , Woodward E. Malcolm S. , Ribeiro Lourenço , Netting Jane , Clewley Daniel , Groom Steve B.
Published: November 2023

The Atlantic Meridional Transect (AMT) program (www.amt-uk.org) provides the perfect opportunity to observe the phytoplankton community size structure over a long latitudinal transect 50oN to 50oS, thereby covering the most important latitude-related basin-scale environmental gradients of the Atlantic Ocean. This work presents cell abundance data of phytoplankton taxa recently collected during cruises AMT28 and 29 (in 2018 and 2019, respectively) using flow cytometer and microscope observations, as well as the pigment composition of the community, to assess the abundance and spatial distribution of taxonomic groups across the Atlantic. The community size structure showed a clear consistency between cruises at large spatial scale, with a dominance of picoplanktonic Cyanobacteria in oceanic gyres, an increase in all groups in the equatorial upwelling region, and high biomass of microplankton size class at higher latitudes. Phytoplankton carbon biomass for oceanographic provinces, ranged from median values of 10 to 47 mg Carbon m-3, for the oligotrophic gyres, and South Atlantic (45°S-50oS), respectively. Satellite images of total chlorophyll a (as a proxy for phytoplankton biomass) as well as the relative contribution of the three phytoplankton size classes were produced for both cruises, and despite the small number of matchups, statistically agreed well with in situ size classes estimated as carbon biomass, constituting the first attempt in the literature to match satellite size classes with in situ data derived from cell abundance. The comparison of community structure between recent cruises (2019, 2018, 2015) and earlier ones (1995-1998) indicates a decrease in the number of diatom-bloom forming species, and an increase in Dinoflagellates, whereas nitrogen-fixing Trichodesmium abundance in tropical Atlantic remains constant. Within the recent period, a relative increase in the median values of picoplankton fraction was seen in SATL region, counterbalanced by a decrease in both nano- and microplankton fractions. Additionally, this study includes a database of species identified by microscopy, which had been interrupted for 20 years, providing a basis for long-term series of phytoplankton species.
Assessing the efficiency of microplastics extraction methods for tropical beach sediments and matrix preparation for experimental controls
Authors: Bonita Jan Danielle P. , Gomez Norchel Corcia F. , Onda Deo Florence L.
Published: November 2023

Introduction

Microplastic pollution has become a global issue, eliciting attention not just from the scientific community but also both from the public and governmental bodies. Drawing data-driven policies and interventions, however, remain difficult due to the severely lacking baseline information from different environments such as beaches. One of the challenges in doing baseline studies is the lack of harmonized methodologies that will allow for comparison of results, integration of data, and its effective translation to evidence-based policies. Emphasis on quality control measures among baselining efforts through the proper implementation of experimental controls is also lacking.

Methodology

To address these gaps, we compared methodologies for preparing the sediment matrix for experimental controls, as well as evaluated protocols for extracting microplastics from tropical beach sediments. Beach sediments were collected, dried, sieved, and spiked with known amounts of microplastics of different polymer types. The removal and extraction efficiencies of the protocols being compared were evaluated.

Results and discussion

Our results showed that subjecting beach sediments to a furnace at 550° C for 4 hours is the most efficient way to remove plastic contamination, implying its applicability for preparing experimental controls. Meanwhile, a modified version of Masura et al. (2015), one of the widely cited methodologies for microplastics extraction, exhibited the highest mean extraction efficiency (99.05 ± 0.82%) among the protocols being compared. Results of this work will be useful in identifying methods that can be adopted and utilized for research and baselining efforts not just in the Philippines but also in Southeast Asia. This will also be helpful in the harmonization of methods, data reporting, and even skills as implemented through the regional and national action plans to address marine plastic pollution.
Changes in axial length in anisometropic children wearing orthokeratology lenses
Authors: Qin Jian , Qing Huiling , Ji Na , Lyu Tianbin , Ma Hui , Shi Menghai , Yu Shiao , Ma Conghui , Fu Aicun
Published: November 2023

Purpose

There is a particular anisometropia occurring in one eye with myopia, while the other eye has very low myopia, emmetropia, or very low hyperopia. It is unclear how the binocular axial length changes when these children wear unilateral OK lenses only in the more myopic eyes. This study investigates the changes in the axial elongation of both eyes.

Methods

This is a 1-year retrospective study. In total, 148 children with myopic anisometropia were included. The more myopic eyes were wearing orthokeratology lenses (treated eyes), whereas the contralateral eyes were not indicated for visual correction (untreated eyes). The untreated eyes were classified into three subgroups based on the spherical equivalent refraction (SER): low myopia (≤ -0.50 D, n = 37), emmetropia (+0.49 to −0.49 D, n = 76), and low hyperopia (≥0.50 D, n = 35). Changes in the axial length (AL) were compared between the untreated and treated eyes and among the three subgroups.

Results

The axial elongation was 0.14 ± 0.18 mm and 0.39 ± 0.27 mm in all treated and untreated eyes, respectively (p < 0.001). The interocular AL difference decreased significantly from 1.09 ± 0.45 mm at the baseline to 0.84 ± 0.52 mm at 1 year (p < 0.001). The baseline median (Q1, Q3) SER of the untreated eyes were −0.75 D (−0.56, −0.88 D), 0.00 D (0.00, −0.25 D), and +0.75 D (+1.00, +0.62 D) in low myopia, emmetropia, and low hyperopia subgroups, respectively. The axial elongation was 0.14 ± 0.18 mm, 0.15 ± 0.17 mm, and 0.13 ± 0.21 mm (p = 0.92) in the treated eyes and 0.44 ± 0.25 mm, 0.35 ± 0.24 mm, and 0.41 ± 0.33 mm in the untreated eyes (p = 0.11) after 1 year. Multivariate linear regression analyses only showed significant differences in axial elongation between the emmetropia and low myopia subgroups of untreated eyes (p = 0.04; p > 0.05 between other subgroups).

Conclusion

Unilateral orthokeratology lenses effectively reduced axial elongation in the more myopic eyes and reduced interocular AL differences in children with myopic anisometropia. The refractive state of the untreated eyes did not affect the axial elongation of the more myopic eye wearing the orthokeratology lens. In the untreated eyes, AL increased faster in the low myopia subgroup than in the emmetropia subgroup.
Ideal serum non-ceruloplasmin bound copper prediction for long-term treated patients with Wilson disease: a nomogram model
Authors: Tao Zhuang , Yang Pingping , Zhou Jiafeng , Wang Rui , Jiang Zhenzhen , Han Hui , Wang Meixia
Published: November 2023

Purpose

This study aimed to explore the factors associated with the optimal serum non-ceruloplasmin bound copper (NCBC) level and develop a flexible predictive model to guide lifelong therapy in Wilson disease (WD) and delay disease progression.

Methods

We retrospectively collected clinical data from 144 patients hospitalized in the Encephalopathy Center of the first affiliated hospital of Anhui University of Chinese Medicine between May 2012 and April 2023. Independent variables were selected using variate COX and LASSO regressions, followed by multivariate COX regression analysis. A predictive nomogram was constructed and validated using the concordance index (C-index), calibration curves, and clinical decision curve analysis, of which nomogram pictures were utilized for model visualization.

Results

A total of 61 (42.36%) patients were included, with an average treatment duration of 55.0 (range, 28.0, 97.0) months. Multivariate regression analysis identified several independent risk factors for serum NCBC level, including age of diagnosis, clinical classification, laminin liver stiffness measurement, and copper to zinc ratio in 24-h urinary excretion. The C-index indicated moderate discriminative ability (48 months: 0.829, 60 months: 0.811, and 72 months: 0.819). The calibration curves showed good consistency and calibration; clinical decision curve analysis demonstrated clinically beneficial threshold probabilities at different time intervals.

Conclusion

The predictive nomogram model can predict serum NCBC level; consequently, we recommend its use in clinical practice to delay disease progression and improve the clinical prognosis of WD.
Highly efficient synergistic activity of an α-L-arabinofuranosidase for degradation of arabinoxylan in barley/wheat
Authors: Wen Jiaqi , Miao Ting , Basit Abdul , Li Qunhong , Tan Shenglin , Chen Shuqing , Ablimit Nuraliya , Wang Hui , Wang Yan , Zheng Fengzhen , Jiang Wei
Published: November 2023

Here, an α-L-arabinofuranosidase (termed TtAbf62) from Thermothelomyces thermophilus is described, which efficiently removes arabinofuranosyl side chains and facilitates arabinoxylan digestion. The specific activity of TtAbf62 (179.07 U/mg) toward wheat arabinoxylan was the highest among all characterized glycoside hydrolase family 62 enzymes. TtAbf62 in combination with endoxylanase and β-xylosidase strongly promoted hydrolysis of barley and wheat. The release of reducing sugars was significantly higher for the three-enzyme combination relative to the sum of single-enzyme treatments: 85.71% for barley hydrolysis and 33.33% for wheat hydrolysis. HPLC analysis showed that TtAbf62 acted selectively on monosubstituted (C-2 or C-3) xylopyranosyl residues rather than double-substituted residues. Site-directed mutagenesis and interactional analyses of enzyme–substrate binding structures revealed the catalytic sites of TtAbf62 formed different polysaccharide-catalytic binding modes with arabinoxylo-oligosaccharides. Our findings demonstrate a “multienzyme cocktail” formed by TtAbf62 with other hydrolases strongly improves the efficiency of hemicellulose conversion and increases biomass hydrolysis through synergistic interaction.
Pathogenic invasive microbes Trichoderma pleuroticola transform bacterial and fungal community diversity in Auricularia cornea crop production system
Authors: Ye Lei , Zhang Bo , Zhang Lingzi , Yang Xuezhen , Tan Wei , Zhang Xiaoping , Li Xiaolin
Published: November 2023

Pathogenic invasion of Trichoderma pleuroticola profoundly altered microflora in the Auricularia cornea crop production system, impacting diversity and composition in both artificial bed-log and fruiting bodies. A more complex ecological network between the diseased and healthy bodies. Researchers still have poor knowledge about how the important agricultural relationship between the composition of the microbiome of the artificial bed-log and the fruiting bodies is infected by the pathogenic invasive microbes T. pleuroticola, but this knowledge is crucial if we want to use or improve it. Here, we investigated 8 groups (48 biological samples) across 5 growth stages of the A. cornea production system using metagenomic technology. Diseased and healthy fruiting bodies exhibited distinct microbial compositions, while core members in artificial bed-logs remained stable. Core microbiota analysis highlighted Pseudomonas and Pandoraea bacterial genera, as well as Sarocladium, Cephalotrichum, Aspergillus, and Mortierella fungal genera as biomarker species after the bodies were treated with the pathogenic invasive microbes T. pleuroticola. In diseased bodies, these core members upregulated pathways including polymyxin resistance, L-arginine degradation II, superpathway of L-arginine and L-ornithine degradation, glucose degradation (oxidative), glucose and glucose-1-phosphate degradation, promoting fruit spoilage. Our data confirm that T. pleuroticola plays an important role in the early stages of disease development in the A. cornea crop generation system. The exposed volatile core microbiome may play an important role in accelerating T. pleuroticola-induced decay of fruiting bodies.
Rapid detection of grass carp reovirus type 1 using RPA-based test strips combined with CRISPR Cas13a system
Authors: Li Huaming , Cao Xinyue , Chen Ruige , Guang Min , Xu Mengran , Wu Xiaomin , Yang Rongrong , Lei Liancheng , Zhang Fuxian
Published: November 2023

Introduction

Due to the existence of grass carp reovirus (GCRV), grass carp hemorrhagic disease occurs frequently, and its high pathogenicity and infectivity are great challenges to the aquaculture industry. As a highly pathogenic pathogen, the outbreak of hemorrhagic disease often causes tremendous economic losses. Therefore, it is important to rapidly and accurately detect GCRV on site to control timely.

Methods

In this study, recombinant enzyme amplification (RPA) combined with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a system was employed to establish a method to detect the vp7 gene of grass carp reovirus type 1. This method can be adopted for judging the results by collecting fluorescence signal, ultraviolet excitation visual fluorescence and test strip.

Results

Combined with the RPA amplification experiment, the detection limit of the RPA-CRISPR method can reach 7.2 × 101 copies/μL of vp7 gene per reaction, and the detection process can be completed within 1 h. In addition, this method had no cross-reaction with the other 11 common aquatic pathogens. Then, the performance of the RPA-CRISPR/Cas13a detection method was evaluated by comparing it with the real-time fluorescence quantitative PCR detection method of clinical samples. The results of RPA-CRISPR/Cas13a detection were shown to be in consistence with the results obtained from the real-time fluorescence quantitative PCR detection. The coincidence rate of this method with 26 GCRV clinical samples was 92.31%.

Discussion

In summary, this method has high sensitivity, specificity and on-site practicability for detecting GCRV type 1, and has great application potential in on-site GCRV monitoring.
Automatic MRI volumetry in asymptomatic cases at risk for normal pressure hydrocephalus
Authors: Haller Sven , Montandon Marie-Louise , Rodriguez Cristelle , Herrmann François R. , Giannakopoulos Panteleimon
Published: November 2023

The occurrence of significant Alzheimer’s disease (AD) pathology was described in approximately 30% of normal pressure hydrocephalus (NPH) cases, leading to the distinction between neurodegenerative and idiopathic forms of this disorder. Whether or not there is a specific MRI signature of NPH remains a matter of debate. The present study focuses on asymptomatic cases at risk for NPH as defined with automatic machine learning tools and combines automatic MRI assessment of cortical and white matter volumetry, risk of AD (AD-RAI), and brain age gap estimation (BrainAge). Our hypothesis was that brain aging and AD process-independent volumetric changes occur in asymptomatic NPH-positive cases. We explored the volumetric changes in normal aging-sensitive (entorhinal cortex and parahippocampal gyrus/PHG) and AD-signature areas (hippocampus), four control cortical areas (frontal, parietal, occipital, and temporal), and cerebral and cerebellar white matter in 30 asymptomatic cases at risk for NPH (NPH probability >30) compared to 30 NPH-negative cases (NPH probability <5) with preserved cognition. In univariate regression models, NPH positivity was associated with decreased volumes in the hippocampus, parahippocampal gyrus (PHG), and entorhinal cortex bilaterally. The strongest negative association was found in the left hippocampus that persisted when adjusting for AD-RAI and Brain Age values. A combined model including the three parameters explained 36.5% of the variance, left hippocampal volumes, and BrainAge values, which remained independent predictors of the NPH status. Bilateral PHG and entorhinal cortex volumes were negatively associated with NPH-positive status in univariate models but this relationship did not persist when adjusting for BrainAge, the latter remaining the only predictor of the NPH status. We also found a negative association between bilateral cerebral and cerebellar white matter volumes and NPH status that persisted after controlling for AD-RAI or Brain Age values, explaining between 50 and 65% of its variance. These observations support the idea that in cases at risk for NPH, as defined by support vector machine assessment of NPH-related MRI markers, brain aging-related and brain aging and AD-independent volumetric changes coexist. The latter concerns volume loss in restricted hippocampal and white matter areas that could be considered as the MRI signature of idiopathic forms of NPH.
Single-layer MoS2 solid-state nanopores for coarse-grained sequencing of proteins
Authors: Urquiola Hernández Andreina , Delarue Patrice , Guyeux Christophe , Nicolaï Adrien , Senet Patrick
Published: November 2023

Proteins are essential biological molecules to use as biomarkers for early disease diagnosis. Therefore, their detection is crucial. In recent years, protein sequencing has become one of the most promising techniques. In particular, solid-state nanopores (SSNs) are powerful platforms for single biological molecule sensing without any labeling and with high sensitivity. Atomically thin two-dimensional (2D) materials with nanometer-sized pores, such as single-layer MoS2, represent the ideal SSN because of their ultimate thinness. Despite the benefits they offer, their use for protein sequencing applications remains very challenging since the fast translocation speed provides a short observation time per single molecule. In this work, we performed extensive molecular dynamics simulations of the translocation of the 20 proteinogenic amino acids through single-layer MoS2 nanopores. From ionic current traces, we characterized peptide-induced blockade levels of current and duration for each of the 20 natural amino acids. Using clustering techniques, we demonstrate that positively and negatively charged amino acids present singular fingerprints and can be visually distinguished from neutral amino acids. Furthermore, we demonstrate that this information would be sufficient to identify proteins using the coarse-grained sequencing technique made of only three amino acid categories depending on their charge. Therefore, single-layer MoS2 nanopores have great potential as sensors for the identification of biomarkers.
Insufficiencies in sensory systems reweighting is associated with walking impairment severity in chronic stroke: an observational cohort study
Authors: Awosika Oluwole O. , Garver Amanda , Drury Colin , Sucharew Heidi J. , Boyne Pierce , Schwab Sarah M. , Wasik Emily , Earnest Melinda , Dunning Kari , Bhattacharya Amit , Khatri Pooja , Kissela Brett M.
Published: November 2023

Background

Walking and balance impairment are common sequelae of stroke and significantly impact functional independence, morbidity, and mortality. Adequate postural stability is needed for walking, which requires sufficient integration of sensory information between the visual, somatosensory, and vestibular centers. “Sensory reweighting” describes the normal physiologic response needed to maintain postural stability in the absence of sufficient visual or somatosensory information and is believed to play a critical role in preserving postural stability after stroke. However, the extent to which sensory reweighting successfully maintains postural stability in the chronic stages of stroke and its potential impact on walking function remains understudied.

Methods

In this cross-sectional study, fifty-eight community-dwelling ambulatory chronic stroke survivors underwent baseline postural stability testing during quiet stance using the modified Clinical test of Sensory Interaction in Balance (mCTSIB) and assessment of spatiotemporal gait parameters.

Results

Seventy-six percent (45/58) of participants showed sufficient sensory reweighting with visual and somatosensory deprivation for maintaining postural stability, albeit with greater postural sway velocity indices than normative data. In contrast, survivors with insufficient reweighting demonstrated markedly slower overground walking speeds, greater spatiotemporal asymmetry, and limited acceleration potential.

Conclusion

Adequate sensory system reweighting is essential for chronic stroke survivors’ postural stability and walking independence. Greater emphasis should be placed on rehabilitation strategies incorporating multisensory system integration testing and strengthening as part of walking rehabilitation protocols. Given its potential impact on outcomes, walking rehabilitation trials may benefit from incorporating formal postural stability testing in design and group stratification.
Use of magnetic source imaging to assess recovery after severe traumatic brain injury—an MEG pilot study
Authors: Sarma Anand Karthik , Popli Gautam , Anzalone Anthony , Contillo Nicholas , Cornell Cassandra , Nunn Andrew M. , Rowland Jared A. , Godwin Dwayne W. , Flashman Laura A. , Couture Daniel , Stapleton-Kotloski Jennifer R.
Published: November 2023

Rationale

Severe TBI (sTBI) is a devastating neurological injury that comprises a significant global trauma burden. Early comprehensive neurocritical care and rehabilitation improve outcomes for such patients, although better diagnostic and prognostic tools are necessary to guide personalized treatment plans.

Methods

In this study, we explored the feasibility of conducting resting state magnetoencephalography (MEG) in a case series of sTBI patients acutely after injury (~7 days), and then about 1.5 and 8 months after injury. Synthetic aperture magnetometry (SAM) was utilized to localize source power in the canonical frequency bands of delta, theta, alpha, beta, and gamma, as well as DC–80 Hz.

Results

At the first scan, SAM source maps revealed zones of hypofunction, islands of preserved activity, and hemispheric asymmetry across bandwidths, with markedly reduced power on the side of injury for each patient. GCS scores improved at scan 2 and by scan 3 the patients were ambulatory. The SAM maps for scans 2 and 3 varied, with most patients showing increasing power over time, especially in gamma, but a continued reduction in power in damaged areas and hemispheric asymmetry and/or relative diminishment in power at the site of injury. At the group level for scan 1, there was a large excess of neural generators operating within the delta band relative to control participants, while the number of neural generators for beta and gamma were significantly reduced. At scan 2 there was increased beta power relative to controls. At scan 3 there was increased group-wise delta power in comparison to controls.

Conclusion

In summary, this pilot study shows that MEG can be safely used to monitor and track the recovery of brain function in patients with severe TBI as well as to identify patient-specific regions of decreased or altered brain function. Such MEG maps of brain function may be used in the future to tailor patient-specific rehabilitation plans to target regions of altered spectral power with neurostimulation and other treatments.
Brief instrument for direct complex functionality assessment: a new ecological tool
Authors: Holz Maila Rossato , Kochhann Renata , da Silva Patrícia Ferreira , Wilson Maximiliano A. , Fonseca Rochele Paz
Published: November 2023

Background

The Direct Assessment of Functional Status (DAFS) is the only instrument validated in Brazil that assesses functionality directly with the patient. However, this clinical tool takes a long time to be administered. This limits its use in hospitals and outpatient clinics that require brief assessment instruments. Additionally, we need to count with a direct assessment because the number of older adults living alone is increasing and we thus lack reliable informants.

Objective

This study aimed to present the development and content validity evidence of a direct complex functionality test for older adults, the Brief Instrument for Direct Functionality Assessment (BIDFA).

Method

A total sample of 30 older adults and eight expert judges took part in the study stages. The BIDFA construction stages were: (1) literature review of functionality instruments; (2) development of seven ecological tasks to evaluate the performance of daily complex activities with the older adults; (3) content analysis by eight expert judges; (4) pilot study with 30 older adults; (5) the ecological analysis of items; (6) focus group analysis; and (7) final version of the BIDFA.

Results

The BIDFA had evidence of content validity with an agreement index of 96.5%. The final version of BIDFA was left with six domains of complex functionality divided into semantic memory and time orientation; shopping skills; executive attention, math and finance skills; organization; planning and procedural memory; and problem-solving. The complex functionality score by BIDFA ranges from 0 to 100 points.

Conclusion

The BIDFA was found to have good content validity by the expert judges and by the ecological analysis of the items by the older adults. The new instrument is expected to help assess the functional status of older adults, in an abbreviated context including complex functionality demands, with a wider range of total and subdomain scores.
Signal processing and stimulation potential within the ascending auditory pathway: a review
Authors: Quimby Alexandra E. , Wei Kimberly , Adewole Dayo , Eliades Steven , Cullen D. Kacy , Brant Jason A.
Published: November 2023

The human auditory system encodes sound with a high degree of temporal and spectral resolution. When hearing fails, existing neuroprosthetics such as cochlear implants may partially restore hearing through stimulation of auditory neurons at the level of the cochlea, though not without limitations inherent to electrical stimulation. Novel approaches to hearing restoration, such as optogenetics, offer the potential of improved performance. We review signal processing in the ascending auditory pathway and the current state of conventional and emerging neural stimulation strategies at various levels of the auditory system.
Vitamin D levels in the pre- and post-COVID-19 pandemic periods in pediatric patients with chronic kidney disease
Authors: Parra-Ortega Israel , Zurita-Cruz Jessie Nallely , Ortiz-Flores Itzel , Romero-Navarro Benjamin , Villasis-Keever Miguel Angel , Martínez Briceida López , Domínguez-Castillo Veronica , Romo-Vázquez José Carlos
Published: November 2023

Introduction

Vitamin D (VD) deficiency is common in children with chronic kidney disease (CKD) because of multiple factors. During the coronavirus disease 2019 (COVID-19) pandemic, it increased because of medicine shortage and no enough medical service for patients with non-COVID-19 diseases.

Objective

To analyze the effects of the COVID-19 pandemic-related lockdown on the serum levels and status of 25-hydroxyvitamin D3 (25-[OH]D) in children with CKD.

Materials and methods

This retrospective study included patients (6–18 years old) who were diagnosed with CKD stage 2–5 and routinely measured for serum VD levels between May 2019 and December 2022. Serum 25-(OH)D levels were measured before, during, and after the pandemic (2019, 2020–2021, and 2022, respectively). The daily dose of cholecalciferol supplementation and the readjustment (if required) were recorded.

Results

This study included 171 patients (median age: 12 years). Before the pandemic, the median serum VD level was 25.0 ng/mL (19.3% VD deficiency). Then, VD supplementation was adjusted to 400–1,200 UI daily in 98.8% (n = 169) of patients. During the pandemic, the median VD level decreased to 22.5 ng/mL (43.3% VD deficiency). Hence, the supplementation was readjusted, and after the pandemic, the level was 28.7 ng/mL (18.7% VD deficiency), indicating a statistically significant increase in serum VD levels from the prepandemic period (p = 0.007).

Conclusion

Decreased serum VD levels and increased VD deficiency frequency were observed in patients with CKD during the COVID-19 but improved after readjustment of supplementation.
Neonatal hypoxic-ischemic encephalopathy after acute carbon monoxide intoxication during pregnancy. A case report and brief review of the literature
Authors: Tuoni Cristina , Nuzzi Giulia , Scaramuzzo Rosa Teresa , Fiori Simona , Filippi Luca
Published: November 2023

Carbon monoxide (CO) poisoning during pregnancy is a rare occurrence, associated with high maternal and fetal mortality rates. As CO can cross the placenta, leading to intrauterine hypoxia, CO intoxication can result in neurological sequelae and neurologic complications in fetuses who survive. We report a case of a preterm newborn acutely exposed to CO in-utero and delivered by emergent cesarean section at the 31st week of gestation due to the severe burns suffered by the mother following an indoor boiler explosion. As CO has serious adverse effects both on the mother and fetus, it is important to recognize and treat poisoning in a timely manner. Despite maternal blood CO levels, CO intoxication at critical stage of central nervous system development can lead to hypoxic-ischemic lesions, thus interdisciplinary care and follow up for these patients are mandatory.
Numerical simulation of upper airway heat transfer in children with mandibular retrognathia during inspiratory process
Authors: Gao Yikai , Liu Hongyu , Liu Na , Zhang Li
Published: November 2023

Introduction

The human upper airway regulates temperature, but its heating capacity remains unclear when the ambient temperature is low and the airway structure is abnormal. Therefore, the purpose of this study was to evaluate the heat transfer characteristics of the upper airway in patients with mandibular retrognathia, and to quantitatively evaluate the influence of ambient temperature on the temperature field of the upper airway, which could provide a valuable reference for the prediction, diagnosis and treatment of respiratory tract related diseases.

Methods

Two typical ambient temperatures of —10 °C and 20 °C were selected to numerically simulate the air flow and heat transfer synchronization in the upper airway model of mandibular retrognathia under quiet breathing and heavy breathing.

Results and discussion

The results showed that the inspired air could not be sufficiently heated after flowing through the upper airway and main trachea in the two breathing states under low temperature conditions, and the inferior bronchus was more stimulated under the state of heavy breathing. In addition, the vortex flow structure in the upper airway can enhance the convective heat transfer effect in the corresponding area.
Exploring the dynamics of intentional sensorimotor desynchronization using phasing performance in music
Authors: Kim Ji Chul
Published: November 2023

Humans tend to synchronize spontaneously to rhythmic stimuli or with other humans, but they can also desynchronize intentionally in certain situations. In this study, we investigate the dynamics of intentional sensorimotor desynchronization using phasing performance in music as an experimental paradigm. Phasing is a compositional technique in modern music that requires musicians to desynchronize from each other in a controlled manner. A previous case study found systematic nonlinear trajectories in the phasing performance between two expert musicians, which were explained by coordination dynamics arising from the interaction between the intrinsic tendency of synchronization and the intention of desynchronization. A recent exploratory study further examined the dynamics of phasing performance using a simplified task of phasing against a metronome. Here we present a further analysis and modeling of the data from the exploratory study, focusing on the various types of phasing behavior found in non-expert participants. Participants were instructed to perform one phasing lap, and individual trials were classified as successful (1 lap), unsuccessful (> 1 laps), or incomplete (0 lap) based on the number of laps made. It was found that successful phasing required a gradual increment of relative phase and that different types of failure (unsuccessful vs. incomplete) were prevalent at slow vs. fast metronome tempi. The results are explained from a dynamical systems perspective, and a dynamical model of phasing performance is proposed which captures the interaction of intrinsic dynamics and intentional control in an adaptive-frequency oscillator coupled to a periodic external stimulus. It is shown that the model can replicate the multiple types of phasing behavior as well as the effect of tempo observed in the human experiment. This study provides further evidence that phasing performance is governed by the nonlinear dynamics of rhythmic coordination. It also demonstrates that the musical technique of phasing provides a unique experimental paradigm for investigating human rhythmic behavior.
Enhancing attachment-based aspects of PCIT for young children with a history of maltreatment
Authors: Belanger Kristine , Gennis Hannah , Ottenbreit Nicole , Racine Nicole
Published: November 2023

Disruptive behavior difficulties, such as aggression, non-compliance, and emotional outbursts, are common among children exposed to maltreatment. Parent–Child Interaction Therapy (PCIT) is an effective parenting intervention for addressing child behavior difficulties, however, treatment retention and engagement among parents remain a concern in the clinical setting. This paper describes how the delivery of an intervention that teaches attachment theory concepts (Circle of Security-Parenting, COS-P) prior to PCIT can increase engagement and retention among parents of maltreated children and inform new coaching practices. A detailed description of how to extend and integrate COS-P concepts with PCIT for maltreated families using specific strategies is provided. Recommendations, limitations, and next steps for research are presented.
How professional training impacts teaching innovation among ideological and political teachers: the mediating and moderating role of basic psychological needs satisfaction
Authors: Cui Can , Yin Yanjun
Published: November 2023

The aim of this study is to explore the impact of professional training on teaching innovation among senior high school ideological and political teachers. By introducing the concept of basic psychological needs satisfaction as a mediating factor, the study seeks to uncover the mechanisms and patterns that manifest in teaching innovation within the Chinese cultural context. To gather empirical data, a questionnaire survey was administered to a sample of 780 teachers in this specialized field. The results showed that providing more professional training is an effective way to enhance teaching innovation. Drawing on Self-Determination Theory, the satisfaction of basic psychological needs (competence, relatedness, and autonomy) was found to play an important role in this process. Competence need satisfaction and relatedness need satisfaction played a mediating role, while autonomy need satisfaction played a moderating role. Based on these findings, several recommendations are provided to support the professional training of senior high school ideological and political teachers and enhance their innovation, including providing personalized training programs, building a mentor system, and allowing greater autonomy in school management.
Impact of COVID-19 restrictions on health and well-being in the United Arab Emirates
Authors: Dalibalta Sarah , Ghader Nariman , Rabah Layal , Shaban Sami , Al Mheiri Noor
Published: November 2023

Background

Restrictions during the COVID-19 pandemic are thought to have impacted both the physical health and well-being of individuals where lockdown was applied. The United Arab Emirates (UAE) was one of the leading countries in implementing the international guidelines to limit the intensely contagious nature of the outbreak.

Aim

To identify the impact of COVID-19 on changes to exercise and general physical activity habits, changes to the consumption of various foods and potential weight gain, as well as any differences in smoking habits among individuals residing in the UAE during the COVID-19 quarantine.

Methods

This is a cross-sectional analytical study that used a quantitative electronic questionnaire sent by the Ministry of Health and Prevention to individuals on its platform in order to collect data on the physical health and well-being of a UAE sample population. A total of 2,362 responses were received to specific questions on physical activity, eating habits, and tobacco use for the period before, during, and after the COVID-19 lockdown. Descriptive statistical analysis was used to display the sample’s demographic data and the changes in physical health and well-being. Paired t-test was used to show the changes in dietary habits.

Results

This study reveals concerning changes in health risk behaviors during the COVID-19 lockdown in the UAE. Physical activity levels declined across mild, moderate and vigorous ranges in most participants. Alarmingly, sedentary behavior dramatically increased with 71% of participants spending an average of 4–8 h per day sitting and over 54% of participants spending more than 4 h watching TV on an average day during lockdown. Fast-food consumption and snacking rose, hence weight gain was observed in over 53% of participants. Smoking habits, especially among cigarette smokers, may have worsened, with 45.2% reporting an increase in cigarette smoking, 16.8% declaring an increase in shisha smoking and 35.3% reporting an increase in smoking other tobacco products. These unfavorable behaviors during confinement could have serious long-term health consequences.

Conclusion

This study demonstrates that long periods of home quarantine may have led to unhealthy consequences that increase the risk of developing disease. This study therefore aims to highlight these health impacts, and recommend strategies and policies that can encourage healthy habits.