bluuebunny/crossref_metadata_2025 huggingface dataset:
Increasing Muscle Mass in Elders through Diet and Exercise: A Literature Review of Recent RCTs
Authors: Voulgaridou Gavriela , Papadopoulou Sophia D. , Spanoudaki Maria , Kondyli Fovi S. , Alexandropoulou Ioanna , Michailidou Stella , Zarogoulidis Paul , Matthaios Dimitris , Giannakidis Dimitrios , Romanidou Maria , Papadopoulou Sousana K.
Published: March 2023

This study aimed to review the current evidence on the independent and combined effects of diet and exercise and their impact on skeletal muscle mass in the elderly population. Skeletal muscle makes up approximately 40% of total body weight and is essential for performing daily activities. The combination of exercise and diet is known to be a potent anabolic stimulus through stimulation of muscle protein synthesis from amino acids. Aging is strongly associated with a generalized deterioration of physiological function, including a progressive reduction in skeletal muscle mass and strength, which in turn leads to a gradual functional impairment and an increased rate of disability resulting in falls, frailty, or even death. The term sarcopenia, which is an age-related syndrome, is primarily used to describe the gradual and generalized loss of skeletal muscle mass (mainly in type II muscle fibers) and function. Multimodal training is emerging as a popular training method that combines a wide range of physical dimensions. On the other hand, nutrition and especially protein intake provide amino acids, which are essential for muscle protein synthesis. According to ESPEN, protein intake in older people should be at least 1 g/kgbw/day. Essential amino acids, such as leucine, arginine, cysteine, and glutamine, are of particular importance for the regulation of muscle protein synthesis. For instance, a leucine intake of 3 g administered alongside each main meal has been suggested to prevent muscle loss in the elderly. In addition, studies have shown that vitamin D and other micronutrients can have a protective role and may modulate muscle growth; nevertheless, further research is needed to validate these claims. Resistance-based exercise combined with a higher intake of dietary protein, amino acids, and/or vitamin D are currently recognized as the most effective interventions to promote skeletal muscle growth. However, the results are quite controversial and contradictory, which could be explained by the high heterogeneity among studies. It is therefore necessary to further assess the impact of each individual exercise and nutritional approach, particularly protein and amino acids, on human muscle turnover so that more efficient strategies can be implemented for the augmentation of muscle mass in the elderly.
Royal Jelly and Chlorella vulgaris Mitigate Gibberellic Acid-Induced Cytogenotoxicity and Hepatotoxicity in Rats via Modulation of the PPARα/AP-1 Signaling Pathway and Suppression of Oxidative Stress and Inflammation
Authors: Khadrawy Sally M. , Mohamed Doaa Sh. , Hassan Randa M. , Abdelgawad Mohamed A. , Ghoneim Mohammed M. , Alshehri Sultan , Shaban Nema S.
Published: March 2023

Gibberellic acid (GA3) is a well-known plant growth regulator used in several countries, but its widespread use has negative effects on both animal and human health. The current study assesses the protective effect of royal jelly (RJ) and Chlorella vulgaris (CV) on the genotoxicity and hepatic injury induced by GA3 in rats. Daily oral administration of 55 mg/kg GA3 to rats for 6 constitutive weeks induced biochemical and histopathological changes in the liver via oxidative stress and inflammation. Co-administration of 300 mg/kg RJ or 500 mg/kg CV with GA3 considerably ameliorated the serum levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase), γGT (gamma-glutamyl transferase), total bilirubin, and albumin. Lowered malondialdehyde, tumor necrosis factor α (TNF-α), and nuclear factor κB (NF-κB) levels along with elevated SOD (superoxide dismutase), CAT (catalase), and GPx (glutathione peroxidase) enzyme activities indicated the antioxidant and anti-inflammatory properties of both RJ and CV. Also, they improved the histological structure and reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions along with up-regulating peroxisome proliferator activated receptor α (PPARα) and down-regulating activator protein 1 (AP-1) gene expression. Additionally, chromosomal abnormalities and mitotic index were nearly normalized after treatment with RJ and CV. In conclusion, RJ and CV can protect against GA3-induced genotoxicity and liver toxicity by diminishing oxidative stress and inflammation, and modulating the PPARα/AP-1 signaling pathway.
Simulating the Enrichment of Fossil Radio Electrons by Multiple Radio Galaxies
Authors: Vazza Franco , Wittor Denis , Brüggen Marcus , Brunetti Gianfranco
Published: March 2023

We simulate the evolution of relativistic electrons injected into the intracluster medium by five radio galaxies. We study the spatial transport and the emission properties of the injected radio plasma over a ∼5 Gyr period, and the sequence of cooling and re-acceleration events experienced by electrons, using a Lagrangian approach joined with a numerical method to model the evolution of momentum spectra of relativistic electrons. When compared with electrons injected by shock waves, electrons injected by radio galaxies (here limited to a single injection event) in our tests are unable to fuel large ∼Mpc-sized radio relics with fossil electrons, as required by current theoretical models, while electrons previously seeded by other shocks can do this. On the other hand, the combination of seeding from radio galaxies and of re-acceleration events from plasma perturbation can produce detectable, small-scale, and filamentary emissions in the proximity (≤100–200 kpc) of radio galaxies.
Treatment-Resistant Schizophrenia, Clozapine Resistance, Genetic Associations, and Implications for Precision Psychiatry: A Scoping Review
Authors: Ying Jiangbo , Chew Qian Hui , McIntyre Roger S. , Sim Kang
Published: March 2023

Treatment-resistant schizophrenia (TRS) is often associated with severe burden of disease, poor quality of life and functional impairment. Clozapine is the gold standard for the treatment of TRS, although it is also known to cause significant side effects in some patients. In view of the burgeoning interest in the role of genetic factors in precision psychiatry, we conducted a scoping review to narratively summarize the current genetic factors associated with TRS, clozapine resistance and side effects to clozapine treatment. We searched PubMed from inception to December 2022 and included 104 relevant studies in this review. Extant evidence comprised associations between TRS and clozapine resistance with genetic factors related to mainly dopaminergic and serotoninergic neurotransmitter systems, specifically, TRS and rs4680, rs4818 within COMT, and rs1799978 within DRD2; clozapine resistance and DRD3 polymorphisms, CYP1A2 polymorphisms; weight gain with LEP and SNAP-25 genes; and agranulocytosis risk with HLA-related polymorphisms. Future studies, including replication in larger multi-site samples, are still needed to elucidate putative risk genes and the interactions between different genes and their correlations with relevant clinical factors such as psychopathology, psychosocial functioning, cognition and progressive changes with treatment over time in TRS and clozapine resistance.
The Relevance of Telomerase and Telomere-Associated Proteins in B-Acute Lymphoblastic Leukemia
Authors: da Mota Tales Henrique Andrade , Camargo Ricardo , Biojone Estefânia Rodrigues , Guimarães Ana Flávia Reis , Pittella-Silva Fabio , de Oliveira Diêgo Madureira
Published: March 2023

Telomeres and telomerase are closely linked to uncontrolled cellular proliferation, immortalization and carcinogenesis. Telomerase has been largely studied in the context of cancer, including leukemias. Deregulation of human telomerase gene hTERT is a well-established step in leukemia development. B-acute lymphoblastic leukemia (B-ALL) recovery rates exceed 90% in children; however, the relapse rate is around 20% among treated patients, and 10% of these are still incurable. This review highlights the biological and clinical relevance of telomerase for B-ALL and the implications of its canonical and non-canonical action on signaling pathways in the context of disease and treatment. The physiological role of telomerase in lymphocytes makes the study of its biomarker potential a great challenge. Nevertheless, many works have demonstrated that high telomerase activity or hTERT expression, as well as short telomeres, correlate with poor prognosis in B-ALL. Telomerase and related proteins have been proven to be promising pharmacological targets. Likewise, combined therapy with telomerase inhibitors may turn out to be an alternative strategy for B-ALL.
Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis
Authors: Xie Limei , Bai Xue , Zhang Hao , Qiu Xianqin , Jian Hongying , Wang Qigang , Wang Huichun , Feng Dedang , Tang Kaixue , Yan Huijun
Published: March 2023

Rose plants are widely cultivated as cut flowers worldwide and have economic value as sources of natural fragrance and flavoring. Rosa ‘Crimson Glory’, whose petals have a pleasant fragrance, is one of the most important cultivars of edible rose plants. Flower storage at low-temperature is widely applied in production to maintain quality; however, chilling results in a decrease in aromatic volatiles. To determine the molecular basis underlying the changes in aromatic volatile emissions, we investigated the changes in volatile compounds, DNA methylation patterns, and patterns of the transcriptome in response to chilling temperature. The results demonstrated that chilling roses substantially reduced aromatic volatile emissions. We found that these reductions were correlated with the changes in the methylation status of the promoters and genic regions of the genes involved in volatile biosynthesis. These changes mainly occurred for CHH (H = A, T, or C) which accounted for 51% of the total methylation. Furthermore, transcript levels of scent-related gene Germacrene D synthase (RhGDS), Nudix hydrolase 1 (RhNUDX1), and Phenylacetaldehyde reductase (RhPAR) of roses were strikingly depressed after 24 h at low-temperature and remained low-level after 24 h of recovery at 20 °C. Overall, our findings indicated that epigenetic regulation plays an important role in the chilling tolerance of roses and lays a foundation for practical significance in the production of edible roses.
Hereditary Tyrosinemia Type 1 Mice under Continuous Nitisinone Treatment Display Remnants of an Uncorrected Liver Disease Phenotype
Authors: Neuckermans Jessie , Lequeue Sien , Claes Paul , Heymans Anja , Hughes Juliette H. , Colemonts-Vroninks Haaike , Marcélis Lionel , Casimir Georges , Goyens Philippe , Martens Geert A. , Gallagher James A. , Vanhaecke Tamara , Bou-Gharios George , De Kock Joery
Published: March 2023

Hereditary tyrosinemia type 1 (HT1) is a genetic disorder of the tyrosine degradation pathway (TIMD) with unmet therapeutic needs. HT1 patients are unable to fully break down the amino acid tyrosine due to a deficient fumarylacetoacetate hydrolase (FAH) enzyme and, therefore, accumulate toxic tyrosine intermediates. If left untreated, they experience hepatic failure with comorbidities involving the renal and neurological system and the development of hepatocellular carcinoma (HCC). Nitisinone (NTBC), a potent inhibitor of the 4-hydroxyphenylpyruvate dioxygenase (HPD) enzyme, rescues HT1 patients from severe illness and death. However, despite its demonstrated benefits, HT1 patients under continuous NTBC therapy are at risk to develop HCC and adverse reactions in the eye, blood and lymphatic system, the mechanism of which is poorly understood. Moreover, NTBC does not restore the enzymatic defects inflicted by the disease nor does it cure HT1. Here, the changes in molecular pathways associated to the development and progression of HT1-driven liver disease that remains uncorrected under NTBC therapy were investigated using whole transcriptome analyses on the livers of Fah- and Hgd-deficient mice under continuous NTBC therapy and after seven days of NTBC therapy discontinuation. Alkaptonuria (AKU) was used as a tyrosine-inherited metabolic disorder reference disease with non-hepatic manifestations. The differentially expressed genes were enriched in toxicological gene classes related to liver disease, liver damage, liver regeneration and liver cancer, in particular HCC. Most importantly, a set of 25 genes related to liver disease and HCC development was identified that was differentially regulated in HT1 vs. AKU mouse livers under NTBC therapy. Some of those were further modulated upon NTBC therapy discontinuation in HT1 but not in AKU livers. Altogether, our data indicate that NTBC therapy does not completely resolves HT1-driven liver disease and supports the sustained risk to develop HCC over time as different HCC markers, including Moxd1, Saa, Mt, Dbp and Cxcl1, were significantly increased under NTBC.
The Complete Mitochondrial Genome of Homophyllia bowerbanki (Scleractinia, Lobophylliidae): The First Sequence for the Genus Homophyllia
Authors: Tian Peng , Wang Wei , Xu Ziqing , Cao Bingbing , Jia Zhiyu , Sun Fucheng , Xiao Jiaguang , Niu Wentao
Published: March 2023

Reef-building coral species of the order Scleractinia play an important role in shallow tropical seas by providing an environmental base for the ecosystem. The molecular data of complete mitochondrial genome have become an important source for evaluating phylogenetic and evolutionary studies of Scleractinia. Here, the complete mitogenome of Homophyllia bowerbanki (Milne Edwards and Haime, 1857), collected from Nansha Islands of the South China Sea, was sequenced for the first time through a next-generation sequencing method. H. bowerbanki is the first species of its genus for which the mitogenome was sequenced. This mitogenome was 18,154 bp in size and included two transfer RNA genes (tRNAs), 13 protein-coding genes (PCGs), and two ribosomal RNA genes (rRNAs). It showed a similar gene structure and gene order to the other typical scleractinians. All 17 genes were encoded on the H strand and the total GC content was 33.86% in mitogenome. Phylogenetic analysis (maximum likelihood tree method) showed that H. bowerbanki belonged to the “Robust” clade and clustered together with other two species in the family Lobophylliidae based on 13 PCGs. The mitogenome can provide significant molecular information to clarify the evolutionary and phylogenetic relationships between stony corals and to facilitate their taxonomic classification; it can also support coral species monitoring and conservation efforts.
Ancient DNA of the Don-Hares Assumes the Existence of Two Distinct Mitochondrial Clades in Northeast Asia
Authors: Sharko Fedor , Slobodova Natalia , Boulygina Eugenia , Cheprasov Maksim , Gladysheva-Azgari Maria , Tsygankova Svetlana , Rastorguev Sergey , Novgorodov Gavril , Boeskorov Gennady , Grigorieva Lena , Hwang Woo Suk , Tikhonov Alexei , Nedoluzhko Artem
Published: March 2023

Paleoclimatic changes during the Pleistocene–Holocene transition is suggested as a main factor that led to species extinction, including the woolly mammoth (Mammuthus primigenius), Steller’s sea cow (Hydrodamalis gigas) and the Don-hare (Lepus tanaiticus). These species inhabited the territory of Eurasia during the Holocene, but eventually went extinct. The Don-hare is an extinct species of the genus Lepus (Leporidae, Lagomorpha), which lived in the Late Pleistocene–Early Holocene in Eastern Europe and Northern Asia. For a long time, the Don-hare was considered a separate species, but at the same time, its species status was disputed, taking into account both morphological data and mitochondrial DNA. In this study, mitochondrial genomes of five Don-hares, whose remains were found on the territory of Northeastern Eurasia were reconstructed. Firstly, we confirm the phylogenetic proximity of the “young” specimens of Don-hare and mountain or white hare, and secondly, that samples older than 39 Kya form a completely distinct mitochondrial clade.
SESN2 Could Be a Potential Marker for Diagnosis and Prognosis in Glioma
Authors: Xu Lingdan , Liu Zelin , Wang Huihui , Lu Jiyuan , Xu Jia , Meng Yucheng , Huang Ke , Liu Bin
Published: March 2023

(1) Background: Glioma is among the most common brain tumors, and is difficult to eradicate with current therapeutic strategies due to its highly invasive and aggressive characteristics. Sestrin2 (SESN2) is an autophagy inducer. The effect of SESN2 on glioma is controversial and unclear. (2) Methods: We downloaded related RNA-seq data from the TCGA and GTEx databases. Bioinformatic analyses including differential gene expression analysis, KM survival curve analysis, univariate and multivariate Cox regression analyses, nomogram analysis, ROC curve analysis, gene function enrichment analysis, and immune cell infiltration analysis were conducted. In addition, data from the Human Protein Atlas (HPA) database were collected to validate SESN2 expression in glioma. (3) Results: In comparison with normal tissue, expression of SESN2 in glioma tissue was higher, and those with higher expressions had significantly lower overall survival rates. The results of univariate Cox regression analyses showed that SESN2 can be a disadvantageous factor in poor glioma prognosis. Both nomograms and ROC curves confirmed these findings. Meanwhile, according to gene function analysis, SESN2 may be involved in immune responses and the tumor microenvironment (TME). Based on the HPA database results, SESN2 is localized in the cytosol and shows high expression in glioma. (4) Conclusions: The expression of SESN2 in gliomas was positively relevant to a poorer prognosis, suggesting that SESN2 could be used as a prognostic gene.
Complete Chloroplast Genome of Four Thai Native Dioscorea Species: Structural, Comparative and Phylogenetic Analyses
Authors: Wonok Warin , Sudmoon Runglawan , Tanee Tawatchai , Lee Shiou Yih , Chaveerach Arunrat
Published: March 2023

The chloroplast genomes of Dioscorea brevipetiolata, D. depauperata, D. glabra, and D. pyrifolia are 153,370–153,503 bp in size. A total of 113 genes were predicted, including 79 protein-coding sequences (CDS), 30 tRNA, and four rRNA genes. The overall GC content for all four species was 37%. Only mono-, di-, and trinucleotides were present in the genome. Genes adjacent to the junction borders were similar in all species analyzed. Eight distinct indel variations were detected in the chloroplast genome alignment of 24 Dioscorea species. At a cut-off point of Pi = 0.03, a sliding window analysis based on 25 chloroplast genome sequences of Dioscorea species revealed three highly variable regions, which included three CDS (trnC, ycf1, and rpl32), as well as an intergenic spacer region, ndhF-rpl32. A phylogenetic tree based on the complete chloroplast genome sequence displayed an almost fully resolved relationship in Dioscorea. However, D. brevipetiolata, D. depauperata, and D. glabra were clustered together with D. alata, while D. pyrifolia was closely related to D. aspersa. As Dioscorea is a diverse genus, genome data generated in this study may contribute to a better understanding of the genetic identity of these species, which would be useful for future taxonomic work of Dioscorea.
Lack of Association of Polymorphism Located Upstream of ABCA1 (rs2472493), in FNDC3B (rs7636836), and Near ANKRD55–MAP3K1 Genes (rs61275591) in Primary Open-Angle Glaucoma Patients of Saudi Origin
Authors: Kondkar Altaf A. , Sultan Tahira , Azad Taif A. , Osman Essam A. , Almobarak Faisal A. , Lobo Glenn P. , Al-Obeidan Saleh A.
Published: March 2023

Polymorphisms rs2472493 near ABCA1, rs7636836 in FNDC3B, and rs61275591 near the ANKRD55–MAP3K1 genes were previously reported to exhibit genome-wide significance in primary open-angle glaucoma (POAG). Since these polymorphisms have not been investigated in the Arab population of Saudi Arabia, we examined their association with POAG in a Saudi cohort. Genotyping was performed in 152 POAG cases and 246 controls using Taqman real-time assays and their associations with POAG and clinical markers, such as intraocular pressure, cup/disc ratio, and the number of antiglaucoma medications, were tested by statistical methods. There was no association observed between POAG and the minor allele frequencies of rs2472493[G], rs7636836[T], or rs61275591[A]. None of the genetic models such as co-dominant, dominant, recessive, over-dominant, and log-additive demonstrated any genotype link. The Rs2472493 genotype showed a modest association (p = 0.044) with the number of antiglaucoma medications in the POAG group, but no significant genotype effect on post hoc analysis. In addition, a G-T allelic haplotype of rs2472493 (ABCA1) and rs7636836 (FNDC3B) did show an over two-fold increased risk of POAG (odds ratio = 2.18), albeit non-significantly (p = 0.092). Similarly, no other allelic haplotype of the three variants showed any significant association with POAG. Our study did not replicate the genetic association of rs2472493 (ABCA1), rs763683 (FNDC3B), and rs61275591 (ANKRD55–MAP3K1) in POAG and related clinical phenotypes, suggesting that these polymorphisms are not associated with POAG in a Saudi cohort of Arab ethnicity. However, large population-based multicenter studies are needed to validate these results.
A Late Triassic Nuculanoid Clam (Bivalvia: Nuculanoidea) and Associated Mollusks: Implications for Luning Formation (Nevada, USA) Paleobathymetry
Authors: McMenamin Mark A. S.
Published: March 2023

A silicified, thick-shelled, smooth-surfaced nuculanoid bivalve has been recovered using acid maceration of the Late Triassic (Carnian–Norian) strata of the Luning Formation, Nevada. Comparable modern nuculanoid clams inhabit water depths from 525 to 2562 m, and the living clam (an undescribed species of Pseudoneilonella from Caleta Sierra, Coquimbo, Chile) most similar to the fossil lives at 878–933 m. The Triassic nuculanoid clam (possibly a neilonellid) is inferred here to have inhabited marine waters at approximately 1000 m deep during the deposition of the Shaly Limestone Member of the Luning Formation. The acid maceration sample also produced a silicified specimen of an abyssochrysoid gastropod. The most similar living species to the fossil snail is Abyssochrysos brasilianus, an abyssochrysoid known to occur in water depths from 1540 to 620 m. This depth range also suggests an approximate 1000 m depositional depth for the silicified fossil-producing acid maceration sample from the Luning Formation. These new fossil discoveries falsify hypotheses that the ichthyosaurs (Shonisaurus popularis) of Berlin-Ichthyosaur State Park, Nevada, USA, were deposited, respectively, in either shoreline deposits or in strata that accumulated above the storm wave base. Evidence is also presented here for the existence of a giant Triassic cephalopod that, by comparison with the modern Mesonychoteuthis, preferred water depths of approximately 1000 m.
The Influence of Dutch Genre Painting in Emblematic Prints: Jan Luyken’s Des Menschen, Begin, Midden en Einde (1712)
Authors: Sohn Sooyun
Published: March 2023

This paper examines Dutch printmaker Jan Luyken’s visual strategy represented in his emblem book, Des Menschen Begin, Midden en Einde (1712). As a poet as well as a printmaker, Luyken wrote a poem in this book and produced image prints by himself. Jan Luyken has long been omitted from surveys of Dutch art due to the absence of archival evidence about his life and works. The themes that Luyken employed in his prints, such as parents’ virtue, mother and child, and children’s play, and his genre style, including doorsien, are all examples of contemporary pictorial devices of genre painting prevalent in Luyken’s time. An analysis of the similarities between Des Menschen Begin, Midden en Einde and contemporary genre paintings demonstrates that Luyken’s prints coincided with the development of Dutch Golden Age art. Luyken consciously employed a strategy of incorporating trendy interior items and idealized figures to make his pieces more attractive to his contemporaneous buyers. This is contrary to evaluations of him as an outdated artist indifferent to the contemporary art world.
On the Use of White Marbles in Roman Forum Sempronii (Fossombrone, Marche, Italy): An Overview of the Archaeometric Data on Architectural Elements
Authors: Taelman Devi , Mei Oscar , Antonelli Fabrizio
Published: March 2023

This paper presents the results of the characterisation, identification, provenance determination, and quantification of the white, greco scritto-like and polychrome marbles used for architectural purposes at Forum Sempronii, a Roman town in the Marche region of Italy. Samples of five fragments of white marble and one fragment of a greco scritto-like marble have been studied using mineralogical and petrographic observations and stable isotope ratio analysis (SIRA) of carbon and oxygen. Polychrome marbles were macroscopically identified by comparison with reference samples and available image databases. Analytical results of the white and greco scritto-like marbles identified the material as coming from the quarries of Carrara (Luni, Italy) and Proconnesos (Marmara, Saraylar, Turkey) for white marble, and of Ephesos-Hasançavuslar for greco scritto-like marble. Further, at least eleven imported polychrome stone varieties were distinguished. The study clearly illustrates that Forum Sempronii had access to the main marble trade networks of the Roman Mediterranean. The early attestation of Proconnesian marble at the town’s augusteum (i.e., Augustan date) is unique for central Adriatic Italy.
Understanding the Social Value of Geelong’s Design and Manufacturing Heritage for Extended Reality
Authors: Antlej Kaja , Cooke Steven , Kelly Meghan , Kennedy Russell , Pikó Lauren , Horan Ben
Published: March 2023

Post-industrial cities often find themselves at a crossroads as to whether to find a new identity or embrace their industrial past. In late 2017, after the closure of major manufacturing plants in the region, the Australian city of Geelong was designated a UNESCO City of Design and embraced a “Clever and Creative” strategy which acknowledged Geelong’s industrial and design past in responding to contemporary technological, demographic, and economic challenges. However, questions remain as to which versions of the past are valued by the local community and how these stories can be shared. To better understand the social value of design and manufacturing heritage in Geelong as well as to get initial feedback on how to interpret this type of heritage through novel immersive extended reality (XR) experiences, the researchers took a community-led approach. This paper reports on the results of the initial online community surveys (N = 55–137) and in-person stakeholder interviews (N = 5) with carefully selected representatives of the local government, education, heritage, tourism, and engineering sectors. The study’s outcome demonstrates the importance of design and manufacturing heritage for the local community’s identity. Moreover, this type of heritage provides a source of inspiration, learning opportunities for future creative problem-solvers, and economic opportunities through tourism. By engaging with the social value of design and manufacturing heritage, this paper argues that more effective and targeted storytelling, game-like applications, and other digital immersive experiences such as extended reality (XR) can be used to better engage with audiences.
Transcriptome Analyses Reveal the Key Regulators of Tomato Compound Leaf Development
Authors: Zhu Guangwu , Ma Chongtian , Yu Shuimiao , Zhang Xueying , Jiang Jing , Liu Xin
Published: March 2023

Leaves are one of the organs involved in plant assimilation and transpiration. Different leaf development processes can result in different leaf shapes. Tomato plants have typical compound leaves. It is helpful to explore the regulatory factors affecting the leaf development and morphogenesis of tomatoes to cultivate varieties with high photosynthetic efficiency. We used the typical tomato leaf shape mutants Petroselinum (Pts), Trifoliate (tf2), and Entire (e), which showed a gradual decrease in leaflet number and compound leaf complexity. Transcriptome sequencing was performed to analyze the key differentially expressed genes (DEGs) among the 3 groups, which revealed 2393, 1366, and 1147 DEGs in Pts/VF36, tf2/CR, and e/AC, respectively. We found 86 overlapping DEGs among the 3 groups. In addition, we found that the mutation of Pts, tf2, and e affected not only leaf morphology but also the wax, fatty acid, and abscisic acid pathways during growth and development. An RT-qPCR analysis during leaf primordium development revealed three transcription factors (bHLH079, WRKY44, and WRKY76) and three hormone-regulated genes (IAA-amino acid hydrolase, Gibberellin2ox7, and Gibberellin20ox) that were differentially expressed in the transcriptome. Using virus-induced gene silencing (VIGS), we observed the leaf shape of VIGS plants and found that bHLH079, IAA-amino acid hydrolase, Gibberellin2ox7, Gibberellin20ox, WRKY44, and WRKY76 were the endogenous regulators influencing tomato compound leaf development. This study provides a promising direction for revealing the molecular regulation mechanism underlying compound leaf development in tomatoes.
Comparison of the Essential Oil Content, Constituents and Antioxidant Activity from Different Plant Parts during Development Stages of Wild Fennel (Foeniculum vulgare Mill.)
Authors: Šunić Ljubomir , Ilić Zoran S. , Stanojević Ljiljana , Milenković Lidija , Stanojević Jelena , Kovač Renata , Milenković Aleksandra , Cvetković Dragan
Published: March 2023

The study was conducted to determine fennel essential oil (FEO) yield, composition, and antioxidant activity during four different maturation stages of umbels with seeds (1st stage: immature-pasty; 2nd stage: premature-waxy; 3rd stage: mature-fully ripe; and 4th stage: seeds only), and leaves of wild fennel (Foeniculum vulgare Mill. subsp. vulgare) from the Montenegro coast. The maximum oil yield was found in premature umbels at the waxy stage (4.76 mL/100 g p.m.) and in fully ripe umbels in the early fruiting stage (5.16 mL/100 g p.m.). Fully ripe seeds contained the lowest FEO (mL/100 g p.m.). The minimum FEO content was found in leaves (0.67%). (E)-anethole (64%), α-phellandrene (11.0%), and fenchone (4.8%) were found to be the main components of the essential oil from immature fennel umbels. (E)-anethole (72.3%), fenchone (9.6%) and methyl chavicol (9.5%) were found to be the main components of the essential oil from premature fennel umbels. (E)-anethole (71.6%), fenchone (10.7%) and methyl chavicol (10.3%) were found to be the main components of the essential oil from mature fully ripe fennel umbels. Fennel seeds were rich in (E)-anethole (75.5%) and fenchone (13.7%). FEO from fennel leaves contained (E)-anethole (32.5%), α-phellandrene (18.8%), p-cymene (17.3%), and β-phellandrene (10.3%) as the main compounds. The antioxidant activity of FEO decreases from leaves (12.37 mg/mL) to seeds (37.20 mg/mL). The degree of DPPH radical neutralization increased with the incubation time. Fennel umbels can be harvested before the fully ripe stage, i.e., at the waxy stage, which considerably reduces seed shedding and losses and increases the essential oil yield.
The Impact of Irrigation Intervals and NPK/Yeast on the Vegetative Growth Characteristics and Essential Oil Content of Lemongrass
Authors: Mahmoud Nourhan , Abdou Mahmoud A. H. , Salaheldin Sabri , Soliman Wagdi S. , Abbas Ahmed M.
Published: March 2023

The growth and quality of medicinal plants are greatly affected by environmental stress, with over half of the world’s agricultural land facing water shortages. This research was conducted over two seasons in 2018 and 2019 and aimed to investigate the influence of varying irrigation intervals and partial replacement of mineral fertilizers with biofertilizer (yeast) on the growth, yield, and essential oil content of lemongrass. The study also looked at the effect of the partial substitution of NPK fertilizer with yeast on lemongrass’s ability to withstand water deficit stress. The results showed that water deficit and reducing NPK levels led to a decline in growth characteristics and relative leaf greenness, which was accompanied by an increase in proline content and essential oil percentage. These findings suggest that lemongrass is sensitive to drought and requires adequate nitrogen fertilization. However, extending the irrigation intervals led to an improvement in essential oil content. To achieve high essential oil yield and maintain productivity while considering environmental factors, it is recommended to reduce mineral fertilizer to 50–75% NPK combined with biofertilizer, and space irrigation intervals every 10–15 days. Further research is needed to improve the growth of lemongrass in water-deficient conditions, particularly on newly reclaimed soils.
Organic Pollutants Associated with Plastic Debris in Marine Environment: A Systematic Review of Analytical Methods, Occurrence, and Characteristics
Authors: Zhao Hongrui , Federigi Ileana , Verani Marco , Carducci Annalaura
Published: March 2023

Plastic pollution has become one of the most serious environmental problems, and microplastics (MPs, particles < 5 mm size) may behave as a vehicle of organic pollutants, causing detrimental effects to the environment. Studies on MP-sorbed organic pollutants lack methodological standardization, resulting in a low comparability and replicability. In this work, we reviewed 40 field studies of MP-sorbed organic contaminants using PRISMA guidelines for acquiring information on sampling and analytical protocols. The papers were also scored for their reliability on the basis of 7 criteria, from 0 (minimum) to 21 (maximum). Our results showed a great heterogeneity of the methods used for the sample collection, MPs extraction, and instruments for chemicals’ identification. Measures for cross-contamination control during MPs analysis were strictly applied only in 13% of the studies, indicating a need for quality control in MPs-related research. The most frequently detected MP-sorbed chemicals were polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and organochlorine pesticides (OCPs). Most of the studies showed a good reliability (>75% of the total score), with 32 papers scoring 16 or higher. On the basis of the collected information, a standardizable protocol for the detection of MPs and MP-sorbed chemicals has been suggested for improving the reliability of MPs monitoring studies.
Personalised and Sustainable IEQ Monitoring: Use of Multi-Modal and Pervasive Technologies
Authors: Coulby Graham , Clear Adrian K. , Jones Oliver , Godfrey Alan
Published: March 2023

Background: Monitoring indoor environmental quality (IEQ) is important to better understand occupant health. Passive IEQ monitoring with digital technologies may provide insightful quantitative data to better inform, e.g., health interventions. Yet, many traditional approaches with known IEQ technologies have limited utility due to high costs or coarse granularity—focusing on the collective rather than individuals. Equally, subjective approaches (e.g., manual surveys) have poor adherence (i.e., are burdensome). There is a need for holistic IEQ measurement techniques that are sustainable (affordable, i.e., low-cost) and personalised. Here, the aim of this case report is to explore the use of low-cost digital approaches to gather personalised quantitative and qualitative data. Methods: This study deploys a personalised monitoring approach with IEQ devices coupled to wearables, weather data, and qualitative data, captured through a post-study interview. Results: The mixed-method, single-case approach gathered data continuously for six months with a reduced burden, by using digital technologies to affirm environmental factors, which were subjectively evaluated by the participant. Quantitative data reinforced qualitative data, removing the need for generalising qualitative findings against a collective. Conclusions: This study showed that the single-case, mixed-method approach used here can provide a holistic approach not previously obtainable with traditional pen-and-paper techniques alone. The use of a low-cost multi-modal device linked with common home and wearable technology suggest a contemporary and sustainable IEQ measurement approach that could inform future work to better determine occupant health.
Importance of Soft Skills in Health Sciences Students and Their Repercussion after the COVID-19 Epidemic: Scoping Review
Authors: Sancho-Cantus David , Cubero-Plazas Laura , Botella Navas Marta , Castellano-Rioja Elena , Cañabate Ros Montserrat
Published: March 2023

Soft skills (SKs) are skills related to the interaction among people and their way of dealing with tasks. Increasingly valued in the workplace, they are especially relevant in health professionals due to the importance of the relationship among them and their patients and families. Given their importance, the university training of healthcare professionals must promote the development of SKs. The COVID-19 pandemic has been a turning point in many areas, changing the learning process and, even more, the use of these soft skills as a fundamental ingredient in human relationships. The aim of this study was to analyse the available evidence regarding SKs in health science students, specifically nursing students, and to describe whether there is a worsening in the development of such skills after the COVID-19 pandemic. According to the PRISMA-ScR methodology for systematic reviews, this study included articles on social skills and possible changes in these skills as a consequence of the pandemic in health sciences students The results highlight the importance of these emotional competences for future nurses, being particularly relevant for communication and emotional self-awareness and showing their influence on academic aspects, such as academic performance or mental health and coping skills. A major limitation of the present study was not considering aspects such as compassion or empathy. However, the novelty provided by this work is the analysis of the changes in SKs produced as a consequence of the pandemic. It is definitely clear that there is a need to enhance emotional intelligence, and thus soft skills, in future health professionals.
A Decision Aid for Postpartum Adolescent Family Planning: A Quasi-Experimental Study in Tanzania
Authors: Mushy Stella E. , Horiuchi Shigeko , Shishido Eri
Published: March 2023

Background: We evaluated the effects of our postpartum Green Star family planning decision aid on the decisional conflict, knowledge, satisfaction, and uptake of long-acting reversible contraception among pregnant adolescents in Tanzania. Methods: We used a facility-based pre–post quasi-experimental design. The intervention arm received routine family planning counseling and the decision aid. The control received only routine family planning counseling. The primary outcome was the change in decisional conflict measured using the validated decision conflict scale (DCS). The secondary outcomes were knowledge, satisfaction, and contraception uptake. Results: We recruited 66 pregnant adolescents, and 62 completed this study. The intervention group had a lower mean score difference in the DCS than in the control (intervention: −24.7 vs. control: −11.6, p < 0.001). The mean score difference in knowledge was significantly higher in the intervention than in the control (intervention: 4.53 vs. control: 2.0, p < 0.001). The mean score of satisfaction was significantly higher in the intervention than in the control (intervention: 100 vs. control: 55.8, p < 0.001). Contraceptive uptake was significantly higher in the intervention [29 (45.3%)] than in the control [13 (20.3%)] (p < 0.001). Conclusion: The decision aid demonstrated positive applicability and affordability for pregnant adolescents in Tanzania.
Environmental Regulation in Evolution and Governance Strategies
Authors: Xing Guangyuan , Zhang Youheng , Guo Ju’e
Published: March 2023

Environmental regulation faces theoretical and practical research challenges on global scale, due to differences such as language and policy environment. Research reflects the beneficial exploration of scholars, policymakers, and enterprises in the cognitive and behavioural norms of economic development, environmental protection, and social governance. This study demonstrated that the relevant research is motivated by the enaction of environmental regulations and discussed the influence of relevant research on the evolution of environmental regulations. Under the assumption that environmental regulations are consistent with related research, this study used 9185 papers in the field of environmental regulation from 2000 to 2019 to construct a research network panorama and explored the development and revelation of environmental regulation. The results revealed that environmental regulation research is motivated by the introduction of policies, and regulation is influenced by research evolution with the path of ‘competitiveness-technological change-innovation.’ In addition, after the twenty-first session of the Conference of the Parties (COP21), the number of studies increased significantly, with the USA in the leading position in the research field. Furthermore, governance strategies were inspired by real events, including the increasing concern with climate change, regional research preferences, and the promotion of information disclosure. These results suggest that environmental governors should focus on climate change, localisation, and mechanisms of information disclosure.
Orthodontic Curricula in Undergraduate Dental Education—A Scoping Review
Authors: Raghavan Shailaja , Abu Alhaija Elham , Ali Kamran
Published: March 2023

Objective: To investigate commonalities and variations in the learning outcomes, curriculum content, assessment methods, and competencies in undergraduate orthodontic curricula globally. Methods: This scoping review followed the updated methodological guidance proposed by the Joanna Briggs Institute and reported in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). A search on electronic databases PubMed, Scopus, and Embase was conducted for the last 25 years. Google Scholar was used to identify eligible unpublished and grey literature. Results: The total number of reports identified was 231. After removal of 62 duplicates, 169 reports were included in the title and abstract screening. Finally, 17 studies were included in the review, which included 13 cross-sectional surveys, three expert panel proceedings, and one discussion paper. Marked variations were reported in undergraduate orthodontic curricula and competency assessments at the level of individual countries, regionally as well as globally. The challenges of imparting competency in orthodontic treatment during undergraduate dental education are also acknowledged. Conclusion: Lack of consistency in undergraduate orthodontic education was evidenced by several Delphi studies aiming to develop a consensus on orthodontic teaching in undergraduate programs. A common message emanating from the available studies on undergraduate orthodontic education seems to emphasize a focus on assessment and diagnosis of the orthodontic treatment needs of patients and a basic understanding of contemporary treatment options to facilitate patient referral.
A Case of Autoimmune Small Fiber Neuropathy as Possible Post COVID Sequelae
Authors: Panagiotides Noel G. , Zimprich Fritz , Machold Klaus , Schlager Oliver , Müller Markus , Ertl Sebastian , Löffler-Stastka Henriette , Koppensteiner Renate , Wadowski Patricia P.
Published: March 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is reported to induce and augment autoimmune processes. Moreover, postinfectious effects of coronavirus disease 2019 (COVID-19) are still poorly understood and often resemble symptoms of the acute infection phase. A patient with swollen extremities was presented to the Department of Angiology at the Medical University of Vienna with complaints of muscle and joint pain, paresthesia, and arterial hypertension with intense headache. Prior to these complaints, she had been suffering from various symptoms since November 2020, following a SARS-CoV-2 infection in the same month. These included recurrent sore throat, heartburn, dizziness, and headache. Paresthesia and muscle and joint pain started in temporal relation to a human papillomavirus (HPV) vaccination. Since the patient was suffering from severe pain, intensive pain management was performed. Skin and nerve biopsies revealed autoimmune small fiber neuropathy. The patient’s condition could be related to COVID-19, as her first symptoms began in temporal relation to the SARS-CoV-2 infection. Furthermore, in the disease course, antinuclear (ANA) and anti-Ro antibodies, as well as anti-cyclic citrullinated peptide (anti-CCP) antibodies, could be detected. Together with the symptoms of xerophthalmia and pharyngeal dryness, primary Sjögren’s syndrome was diagnosed. In conclusion, though biopsy results could not distinguish a cause of the disease, SARS-CoV-2 infection can be discussed as a likely trigger for the patient’s autoimmune reactions.
Living Conditions and the Incidence and Risk of Falls in Community-Dwelling Older Adults: A Multifactorial Study
Authors: Escosura Alegre Irene , Fernández Rodríguez Eduardo José , Sánchez Gómez Celia , García Martín Alberto , Rihuete Galve María Isabel
Published: March 2023

Old age represents a social group that is undergoing continuous expansion. The aging population will be prone to chronic diseases and falls, which is a marker of frailty and a public health problem. This study aims to examine the relationship between living conditions and the prevalence of the risk of falls in older adults within the community. As an observational cross-sectional study, intentional sampling was carried out on residents of the metropolitan area over 75 years of age. The socio-demographic data of the subjects and their history of falls were collected. Additionally, the subjects were evaluated on the risk of falling, basic activities of daily living, such as walking and balance, fragility, and their fear of falling. The statistical analyses used were based on the Shapiro–Wilk test for normality, statistics of central tendency with description, mean (M) and dispersion, standard deviation (SD), bivariate contingency tables for studying the relationships between the variables, and the analysis of Pearson’s relational statistics (χ2). The comparisons of means were resolved by parametric or non-parametric routes. We obtained the following results: 1. The socio-demographic profile of our sample consisted of adults over 75 years of age, the majority of whom were overweight or obese women living in an urban area, specifically in an apartment, and receiving care; 2. Older people in the studied community had mild dependency and frailty, and were also at severe risk of falls; 3. The prevalence of falls was higher in women than in men in this study. Through these results, we confirmed the relationship between living conditions and the prevalence of risk of falls in older adults within the community.