bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Shading and Nutrient Management on Yield Quality of Vegetable Fern
Authors: Thepsilvisut Ornprapa , Iad-ak Rantiya , Chutimanukul Preuk
Published: February 2023

This study investigated the optimization of shading and organic fertilizer applications on vegetable fern growth and yield quality in order to develop guidelines for farmers interested in sustainable vegetable fern production. The experiment was conducted in a split-plot design in RCBD with four replications. There were three main plots; no shading, 75% shading, and 96% shading. The five sub-plots consisted of no fertilizer application (control), chemical fertilizer at a rate of 92.80 kg N ha−1, and cow manure at rates of 92.80, 185.60, and 278.40 kg N ha−1. A comparison between different shading and fertilizer treatments, combined, demonstrated that 75% shading with the application of cow manure at the rate of 185.60 kg N ha−1 was the most appropriate management for vegetable fern production, with the highest marketable yield recorded (1128.54 kg ha−1 month−1) and a 10-fold yield increase compared to the control (no shading and no fertilizer). This treatment also resulted in good yield quality (crunchy and tender), high concentrations of chlorophyll and vitamin C, and a safe amount of nitrate accumulation for consumers.
Evaluation of Pecan [Carya illinoinensis (Wangenh.) K. Koch] Cultivars for Possible Cultivation for Both Fruit and Truffle Production in the Puglia Region, Southeastern Italy
Authors: Ferrara Giuseppe , Lombardini Leonardo , Mazzeo Andrea , Bruno Giovanni Luigi
Published: February 2023

Yield and different nut parameters were measured for two growing seasons on mature (28–29 years) trees of 11 pecan cultivars grown in an experimental orchard located in the Puglia Region, Southeastern Italy. ‘Shoshoni’ and ‘Shawnee’ pecan seedlings were inoculated with three truffle species (Tuber borchii—known as the ‘whitish truffle’, T. aestivum—called the ‘summer truffle’, and T. melanosporum—the common ‘Black truffle’) and investigated for six months. The level of ectomycorrhizal colonization was assessed 6 and 12 months after inoculation. Results indicated that ‘Wichita’, ‘Shoshoni’, and ‘Pawnee’ performed well in the pedoclimatic conditions of the area with a yield higher than 20 kg/tree and a kernel dry weight of ≅3 g. These preliminary yield results suggested that some pecan cultivars could deserve consideration for cultivation in the Puglia Region, whereas others with low yield and a stronger alternate bearing should not be considered. Plant height, number of leaves, chlorophyll content (expressed as a SPAD unit) and stem diameter partially indicated the increase in ecological fitness in truffles-inoculated plants. Successful mycorrhization indicated ‘Shoshoni’ and ‘Shawnee’ as suitable to establish ectomycorrhizal symbiosis with T. aestivum, T. borchii, and T. melanosporum cultivation under Puglia climatic conditions. The results also showed that the applied protocol was adequate to obtain healthy mycorrhized seedlings appropriate for commercialization and plantation for truffles production on pecan in the future.
Inheritance and Innovation of Pottery Sculpture Technique in Shiwan, China: A Grounded Study from Cultural Ecology
Authors: Zhou Liting , Zou Xiaomei , Huang Yongnan , Li Yiyong , Guo Luyao , Fu Junyu
Published: February 2023

Ceramics play an important role in human daily life and production practice. Pottery sculpture technique is the core of ceramic making. However, the production process of traditional ceramics is accompanied by high pollution, which has a great impact on human health and the ecological environment. Rapid development of industrialization has exacerbated this consequence. As the “Pottery Capital of Southern China”, Foshan has been involved in environmental crises while relying on the ceramic industry to develop. Since the 21st century, Foshan has gradually successfully driven to upgrade the city from industrial to culture-led by carrying out positive innovations in Shiwan pottery sculpture technique. Therefore, based on the theoretical perspective of cultural ecology, this paper selects Shiwan pottery sculpture technique as the object, uses Python (Octopus Collector) to obtain data, and applies grounded theory to generate the ecological evolution model. This study discussed how the Shiwan pottery sculpture technique promotes the harmonious coexistence of human beings, industries, and cities in the new cultural ecological environment of the 21st century by exploring and clarifying the interaction and function of different elements in different stages of evolution. Finally, this study not only makes up for the current lack of research on Shiwan’s cultural ecology, but also provides meaningful reference for environmental reform in other industrialized cities.
Suicide before and during the COVID-19 Pandemic: A Systematic Review with Meta-Analysis
Authors: Yan Yifei , Hou Jianhua , Li Qing , Yu Nancy Xiaonan
Published: February 2023

Synthesizing evidence to examine changes in suicide-related outcomes before and during the pandemic can inform suicide management during the COVID-19 crisis. We searched 13 databases as of December 2022 for studies reporting both the pre- and peri-pandemic prevalence of suicidal ideation, suicide attempts, or rate of death by suicide. A random-effects model was used to pool the ratio of peri- and pre-pandemic prevalence of suicidal ideation and attempt (Prevalence Ratio—PR) and rate of death by suicide (Rate Ratio; RR). We identified 51, 55, and 25 samples for suicidal ideation, attempt, and death by suicide. The prevalence of suicidal ideation increased significantly among non-clinical (PR = 1.142; 95% CI: 1.018–1.282; p = 0.024; k = 28) and clinical (PR = 1.134; 95% CI: 1.048–1.227; p = 0.002; k = 23) samples, and pooled estimates differed by population and study design. Suicide attempts were more prevalent during the pandemic among non-clinical (PR = 1.14; 95% CI: 1.053–1.233; p = 0.001; k = 30) and clinical (PR = 1.32; 95% CI: 1.17–1.489; p = 0.000; k = 25) participants. The pooled RR for death by suicide was 0.923 (95% CI: 0.84–1.01; p = 0.092; k = 25), indicating a nonsignificant downward trend. An upward trend of suicidal ideation and suicide attempts was observed during the COVID-19 pandemic, despite suicide rate remaining stable. Our findings suggest that timely prevention and intervention programs are highly needed for non-clinical adult population and clinical patients. Monitoring the real-time and long-run suicide risk as the pandemic evolves is warranted.
What Drives Portuguese Women to Be Physically Active? Associations between Motives and Well-Being Indicators
Authors: Silva Alicia , Antunes Raul , Monteiro Diogo , Jacinto Miguel , Matos Rui , Rodrigues Filipe
Published: February 2023

Motives and self-esteem play crucial roles in shaping personal behavior and emotions and have been shown to impact well-being. However, the association between these constructs has been overlooked in women who seem to be more externally driven to engage in exercise. The present study was carried out with the objective of analyzing the associations between motives for physical exercise, positive and negative activations, and self-esteem of Portuguese women exercising at gyms and fitness centers. The sample consists of 206 women aged between 16 and 68 years old (M = 35.77; SD = 11.47). Participants answered a short sociodemographic questionnaire, the Goal Content for Exercise Questionnaire, the Positive and Negative Affect Schedule, and the Rosenberg Self-esteem Scale. The results showed that the health motive had the highest predictive value (β = 0.24; p < 0.01) on self-esteem and demonstrated a positive and significant correlation with positive activation and self-esteem (p < 0.01). On the other hand, the social recognition motive had the lowest predictive value on self-esteem (β = −0.04; p > 0.05) and demonstrated a non-significant correlation with positive activation and self-esteem (p > 0.05). Looking at the coefficients in the hierarchical regression model, it can be seen that the health motive and positive activation were positively and significantly correlated with self-esteem. This study points to the need to raise awareness about the motives of exercise related to the physical and mental health of Portuguese women. Portuguese women that exercise for health motives display greater perceived self-esteem which is an indication of a greater sense of well-being. While the results are limited to Portuguese women, exercise physiologists assessing exercise motives could provide information on how to prescribe exercise as a means to increase self-esteem, considering the positive activation resulting from this behavior.
Clinical Epigenomic Explanation of the Epidemiology of Cannabinoid Genotoxicity Manifesting as Transgenerational Teratogenesis, Cancerogenesis and Aging Acceleration
Authors: Reece Albert Stuart , Hulse Gary Kenneth
Published: February 2023

As global interest in the therapeutic potential of cannabis and its’ derivatives for the management of selected diseases increases, it is increasingly imperative that the toxic profile of cannabinoids be thoroughly understood in order to correctly assess the balance between the therapeutic risks and benefits. Modern studies across a number of jurisdictions, including Canada, Australia, the US and Europe have confirmed that some of the most worrying and severe historical reports of both congenital anomalies and cancer induction following cannabis exposure actually underestimate the multisystem thousand megabase-scale transgenerational genetic damage. These findings from teratogenic and carcinogenic literature are supported by recent data showing the accelerated patterns of chronic disease and the advanced DNA methylation epigenomic clock age in cannabis exposed patients. Together, the increased multisystem carcinogenesis, teratogenesis and accelerated aging point strongly to cannabinoid-related genotoxicity being much more clinically significant than it is widely supposed and, thus, of very considerable public health and multigenerational impact. Recently reported longitudinal epigenome-wide association studies elegantly explain many of these observed effects with considerable methodological sophistication, including multiple pathways for the inhibition of the normal chromosomal segregation and DNA repair, the inhibition of the basic epigenetic machinery for DNA methylation and the demethylation and telomerase acceleration of the epigenomic promoter hypermethylation characterizing aging. For cancer, 810 hits were also noted. The types of malignancy which were observed have all been documented epidemiologically. Detailed epigenomic explications of the brain, heart, face, uronephrological, gastrointestinal and limb development were provided, which amply explained the observed teratological patterns, including the inhibition of the key morphogenic gradients. Hence, these major epigenomic insights constituted a powerful new series of arguments which advanced both our understanding of the downstream sequalae of multisystem multigenerational cannabinoid genotoxicity and also, since mechanisms are key to the causal argument, inveighed strongly in favor of the causal nature of the relationship. In this introductory conceptual overview, we present the various aspects of this novel synthetic paradigmatic framework. Such concepts suggest and, indeed, indicate numerous fields for further investigation and basic science research to advance the exploration of many important issues in biology, clinical medicine and population health. Given this, it is imperative we correctly appraise the risk–benefit ratio for each potential cannabis application, considering the potency, severity of disease, stage of human development and duration of use.
Endometriosis Symptomatology, Dyspareunia, and Sexual Distress Are Related to Avoidance of Sex and Negative Impacts on the Sex Lives of Women with Endometriosis
Authors: Privitera Georgia , O’Brien Kerry , Misajon RoseAnne , Lin Chung-Ying
Published: February 2023

Background: Endometriosis affects approximately 10% of women and is associated with a range of symptoms including pelvic pain, abnormal bleeding, and painful sexual intercourse. However, very little is known about the relationship between endometriosis-related symptoms and sex. Methods: Women with a diagnosis of endometriosis (n = 2060; mean age = 30 years) completed a questionnaire measuring the frequency of endometriosis symptoms, dyspareunia, sexual distress, avoidance of sex, and the perceived negative impact of endometriosis symptoms on sex life. Results: In bivariate and multivariate logistic regression models with avoidance of sex and perceived negative impact of endometriosis symptoms on sex life as DVs, higher endometriosis symptom frequency, dyspareunia, and sexual distress were associated with greater avoidance of sex and higher perceived negative impact of endometriosis symptoms on sex life. With a two- and three-fold increase in the odds of avoiding sex and reporting a negative impact of endometriosis on sex lives, respectively, for each point increase in dyspareunia. Similarly, there was a 7% to 11% increase in avoidance of sex and the negative impact of endometriosis on sex lives, per one-point increase in symptom frequency and sexual distress. Conclusions: The results highlight the considerable impacts of endometriosis symptomatology on women’s sex lives and wellbeing. Better medical and counselling services may be needed to ameliorate the negative impact of endometriosis on women’s sex lives.
Can Potted Plants Catch Mosquitoes? Applying Rare-Earth Luminescent Materials and Plant Energy to the Development of Innovative Mosquito-Trapping Potted Plants
Authors: Lin Lung-Yin , Chang Yu-Shing
Published: February 2023

Due to the global warming crisis, the spread of various infectious diseases is worsening, with mosquito-borne contagious diseases posing a significant threat. While many residential and public spaces contain plants, often for greening the environment and improving mental and physical well-being, the carbon dioxide released by these plants produces ideal habitats for mosquitoes. Considering the quality of life of urban residents and the development of health-related products simultaneously is an important topic. This study used diverse complementary techniques, such as energy-storing rare-earth luminescent materials, sustainable power generation using plant energy, blue light–emitting diodes, and environmentally friendly fermentation formula, to develop planting products with potential mosquito control functionality. The prototype design for this mosquito-trapping potted plant has been patented. The aim of this paper is to discuss the design principles adopted to improve the defects of existing mosquito-trapping designs, the green energy materials and techniques employed, the architecture configuration of the product prototype, and the test results. By integrating green materials and technology, the prototype can be self-powered without being plugged in to yield conspicuous energy savings. The results showed that the developed multi-function products, combined with the concept of energy sustainability, can improve global public health as well as individuals’ physical and mental health.
Exploring COVID-19 Vaccine Attitudes among Racially and Ethnically Minoritized Communities: Community Partners’ and Residents’ Perspectives
Authors: Martinez Leal Isabel , Njoh Journa , Chen Tzuan A. , Foreman-Hays Faith , Reed Brian C. , Haley Sean A. , Chavez Kerry , Reitzel Lorraine R. , Obasi Ezemenari M.
Published: February 2023

COVID-19 has disproportionately affected Black, Indigenous, and People of Color (BIPOC) communities, yet rates of COVID-19 vaccination remain low among these groups. A qualitative study was undertaken to better understand the factors contributing to low vaccine acceptance among these communities. Seventeen focus groups were conducted in English and Spanish from 8/21 to 9/22, with representatives from five critical community sectors: (1) public health departments (n = 1); (2) Federally Qualified Health Centers (n = 2); (3) community-based organizations (n = 1); (4) faith-based organizations (n = 2); and (5) BIPOC residents in six high-risk, underserved communities in metropolitan Houston (n = 11), for a total of 79 participants, comprising 22 community partners and 57 community residents. A social-ecological model and anti-racism framework were adopted to guide data analysis using thematic analysis and constant comparison, which yielded five key themes: (1) legacy of structural racism: distrust and threat; (2) media misinformation: mass and social; (3) listening and adapting to community needs; (4) evolving attitudes towards vaccination; and (5) understanding alternative health belief systems. Although structural racism was a key driver of vaccine uptake, a notable finding indicated community residents’ vaccine attitudes can be changed once they are confident of the protective benefits of vaccination. Study recommendations include adopting an explicitly anti-racist lens to: (1) listen to community members’ needs and concerns, acknowledge their justified institutional distrust concerning vaccines, and learn community members’ healthcare priorities to inform initiatives built on local data; (2) address misinformation via culturally informed, consistent messaging tailored to communal concerns and delivered by trusted local leaders through multimodal community forums; (3) take vaccines to where people live through pop-up clinics, churches, and community centers for distribution via trusted community members, with educational campaigns tailored to the needs of distinct communities; (4) establish vaccine equity task forces to continue developing sustainable policies, structures, programs and practices to address the structural issues driving vaccine and health inequities within BIPOC communities; and (5) continue investing in an effective infrastructure for healthcare education and delivery, essential for competently responding to the ongoing healthcare and other emergency crises that impact BIPOC communities to achieve racial justice and health equity in the US. Findings underscore the crucial need to provide culturally tailored health education and vaccination initiatives, focused on cultural humility, bidirectionality, and mutual respect to support vaccine re-evaluation.
The United Nations (UN) Card, Identity, and Negotiations of Health among Rohingya Refugees
Authors: Rahman Md Mahbubur , Dutta Mohan J.
Published: February 2023

Being persecuted and expelled from Myanmar, Rohingya refugees are now distributed throughout the world. The Southeast Asian nation of Malaysia has been a preferred destination for Rohingyas fleeing Myanmar’s state-sponsored genocide and more recently in a bid to change their fates from the refugee camps in Bangladesh. Refugees are one of the most vulnerable groups in Malaysia and often face dire circumstances, in which their health and wellbeing are compromised. Amidst a plethora of structural challenges, Rohingya refugees try to claim some of their rights with the aid of the UN card (UNHCR ID cards) in Malaysia. Guided by the culture-centered approach (CCA), this study examined the perspectives and experiences of healthcare among Rohingya refugees while living in Malaysia, now resettled in Aotearoa, New Zealand. The participants’ narratives showed that the UN card not only materialized their refugee status in Malaysia but also offered them a way of living in a world where documents anchor the materiality of health.
Analyzing Subway Operation Accidents Causations: Apriori Algorithm and Network Approaches
Authors: Deng Yongliang , Zhang Ying , Yuan Zhenmin , Li Rita Yi Man , Gu Tiantian
Published: February 2023

Subway operation safety management has become increasingly important due to the severe consequences of accidents and interruptions. As the causative factors and accidents exhibit a complex and dynamic interrelationship, the proposed subway operation accident causation network (SOACN) could represent the actual scenario in a better way. This study used the SOACN to explore subway operation safety risks and provide suggestions for promoting safety management. The SOACN model was built under 13 accident types, 29 causations and their 84 relationships based on the literature review, grounded theory and association rule analysis, respectively. Based on the network theory, topological features were obtained to showcase different roles of an accident or causation in the SOACN, including degree distribution, betweenness centrality, clustering coefficient, network diameter, and average path length. The SOACN exhibits both small-world network and scale-free features, implying that propagation in the SOACN is fast. Vulnerability evaluation was conducted under network efficiency, and its results indicated that safety management should focus more on fire accident and passenger falling off the rail. This study is beneficial for capturing the complex accident safety-risk–causation relationship in subway operations. It offers suggestions regarding safety-related decision optimization and measures for causation reduction and accident control with high efficiency.
The Impact of Living Arrangements on the Prevalence of Falls after Total Joint Arthroplasty: A Comparison between Institutionalized and General Geriatric Population
Authors: Pop Anca Maria , Russu Octav Marius , Zuh Sándor György , Feier Andrei Marian , Pop Tudor Sorin
Published: February 2023

Due to population aging, there is an increasing need for orthopedic surgery, especially total knee arthroplasty (TKA) and total hip arthroplasty (THA). In geriatric patients, postoperative falls are common events which can compromise the success of these expensive procedures. The aim of our study was to assess the influence of living arrangements on the prevalence of postoperative falls following joint replacement. We included 441 patients after TKA or THA, living in nursing homes, alone or with family. The prevalence of falls in the first 2 years (15.2%) was significantly influenced by living arrangements: patients with TKA or THA living alone had three times higher odds of falling compared to those living with family, and institutionalized patients with THA had four times higher odds of falling compared to those living with family. Of 67 patients who fell, 6 (8.9%) needed reintervention. For TKA patients, the fall rates were not significantly different between institutions and family, indicating the interest of nursing homes in offering proper care. However, for the THA group, the results were poorer, emphasizing the need for improvement in postoperative rehabilitation. Further multi-centric studies are required for generalizing the impact of living arrangements on fall prevalence after joint replacement.
Risk Prediction for the Development of Hyperuricemia: Model Development Using an Occupational Health Examination Dataset
Authors: Zheng Ziwei , Si Zhikang , Wang Xuelin , Meng Rui , Wang Hui , Zhao Zekun , Lu Haipeng , Wang Huan , Zheng Yizhan , Hu Jiaqi , He Runhui , Chen Yuanyu , Yang Yongzhong , Li Xiaoming , Xue Ling , Sun Jian , Wu Jianhui
Published: February 2023

OBJECTIVE: Hyperuricemia has become the second most common metabolic disease in China after diabetes, and the disease burden is not optimistic. METHODS: We used the method of retrospective cohort studies, a baseline survey completed from January to September 2017, and a follow-up survey completed from March to September 2019. A group of 2992 steelworkers was used as the study population. Three models of Logistic regression, CNN, and XG Boost were established to predict HUA incidence in steelworkers, respectively. The predictive effects of the three models were evaluated in terms of discrimination, calibration, and clinical applicability. RESULTS: The training set results show that the accuracy of the Logistic regression, CNN, and XG Boost models was 84.4, 86.8, and 86.6, sensitivity was 68.4, 72.3, and 81.5, specificity was 82.0, 85.7, and 86.8, the area under the ROC curve was 0.734, 0.724, and 0.806, and Brier score was 0.121, 0.194, and 0.095, respectively. The XG Boost model effect evaluation index was better than the other two models, and similar results were obtained in the validation set. In terms of clinical applicability, the XG Boost model had higher clinical applicability than the Logistic regression and CNN models. CONCLUSION: The prediction effect of the XG Boost model was better than the CNN and Logistic regression models and was suitable for the prediction of HUA onset risk in steelworkers.
Framework for Integrating Productive, Contributory, and Noncontributory Work with Safe and Unsafe Acts and Conditions
Authors: Brioso Xavier , Calderon-Hernandez Claudia
Published: February 2023

It is common for companies that are in the process of implementing the Last Planner System (LPS) journey to attempt an increase in productive work and a reduction in waste, such as contributory and noncontributory work. Even though the LPS has proven to have a synergy with the health and safety requirements, companies with deficient health and safety management systems tend to classify work involving substandard acts or conditions as standard, and then pretend to benchmark against other companies that are indeed performing safe work. The following work introduces a framework to simultaneously register and analyze productive, contributory, and noncontributory work, with the substandard acts and conditions in a construction site, allowing for the measurement of production and health & safety indicators simultaneously. In the absence of technology that automatically captures these indicators, it is proposed that simultaneous measurements be made through direct inspections and photo and video recording by means of a handheld camera. The proposed continuous improvement framework follows the steps indicated below: (1) defining the productive, contributory, and noncontributory work with surveys performed on the most representative stakeholders of the industry; (2) proposing a new classification of production and safety work; (3) assessing the level of application of the LPS in the company; (4) measuring the indicators; (5) improving the use of the LPS and performing new measurements; (6) statistically linking deadly, serious, and minor accidents, standard and substandard acts, standard and substandard conditions, and productive, contributory, and noncontributory work. This framework was applied to a case study of a building project in Lima and the results were improved simultaneous indicators, especially the health and safety indicators. Automated classification of productive and nonproductive work using technology still represents a challenge.
The Moderating Role of Family Resilience on the Relationship between COVID-19-Related Psychological Distress and Mental Health among Caregivers of Individuals with Eating Disorders in Post-Pandemic China
Authors: Wei Yaohui , Li Zhiqian , Guo Lei , Zhang Lei , Lian Cheng , Yuan Chengmei , Chen Jue
Published: February 2023

COVID-19 has amplified long-standing emotional distress for vulnerable families. While abundant research highlights the importance of resilience under adverse circumstances, little has been undertaken to understand its effectiveness in helping caregivers of individuals with eating disorders (ED) navigate pandemic-related challenges. This paper presents findings of a cross-sectional study investigating the effects of COVID-19-related life disruptions (COLD) and COVID-19-related psychological distress (CORPD) on caregivers’ depression, anxiety and stress, as well as the moderation role of individual resilience (IR) and family resilience (FR) during the post-pandemic period in China. A total of 201 caregivers of individuals experiencing ED participated in our online survey from May 2022 to June 2022. The association between pandemic-related stressors (i.e., COLD and CORPD) and mental health conditions were confirmed. FR moderated the relationship between CORPD and mental health outcomes, while IR independently contributed to low emotional distress. We call for intervention programs strengthening caregivers’ FR and IR, which might benefit both patients and caregivers’ well-being in the post-pandemic period.
The Effects of Service Quality Performance on Customer Satisfaction for Non-Banking Financial Institutions in an Emerging Economy
Authors: Wang Cheng-Kun , Masukujjaman Mohammad , Alam Syed Shah , Ahmad Ismail , Lin Chieh-Yu , Ho Yi-Hui
Published: February 2023

This study aims to explore the effects of service-quality dimensions on the customer satisfaction of non-banking financial institutions in an emerging economy by adopting the renowned SERVPERF model. To verify the proposed model, data was collected from thirteen non-banking financial institutions in Bangladesh using a questionnaire survey with a purposive random sampling method. Through the Smart PLS 2 software, the partial least squares structural equation modelling approach was used to analyze the collected data. Research findings reveal that, among the six dimensions of the revised SERVPERF model, assurance, reliability, responsiveness and tangibility have significant effects on customer satisfaction, but accessibility and empathy do not for the non-banking financial institutions in Bangladesh. According to the research results, implications and suggestions have been discussed for non-banking financial institution managers.
Carrier Transport in Colloidal Quantum Dot Intermediate Band Solar Cell Materials Using Network Science
Authors: Cuadra Lucas , Salcedo-Sanz Sancho , Nieto-Borge José Carlos
Published: February 2023

Colloidal quantum dots (CQDs) have been proposed to obtain intermediate band (IB) materials. The IB solar cell can absorb sub-band-gap photons via an isolated IB within the gap, generating extra electron-hole pairs that increase the current without degrading the voltage, as has been demonstrated experimentally for real cells. In this paper, we model the electron hopping transport (HT) as a network embedded in space and energy so that a node represents the first excited electron state localized in a CQD while a link encodes the Miller–Abrahams (MA) hopping rate for the electron to hop from one node (=state) to another, forming an “electron-HT network”. Similarly, we model the hole-HT system as a network so that a node encodes the first hole state localized in a CQD while a link represents the MA hopping rate for the hole to hop between nodes, leading to a “hole-HT network”. The associated network Laplacian matrices allow for studying carrier dynamics in both networks. Our simulations suggest that reducing both the carrier effective mass in the ligand and the inter-dot distance increases HT efficiency. We have found a design constraint: It is necessary for the average barrier height to be larger than the energetic disorder to not degrade intra-band absorption.
4-Methylumbeliferone Treatment at a Dose of 1.2 g/kg/Day Is Safe for Long-Term Usage in Rats
Authors: Štěpánková Kateřina , Mareková Dana , Kubášová Kristýna , Sedláček Radek , Turnovcová Karolína , Vacková Irena , Kubinová Šárka , Makovický Pavol , Petrovičová Michaela , Kwok Jessica C. F. , Jendelová Pavla , Machová Urdzíková Lucia
Published: February 2023

4-methylumbelliferone (4MU) has been suggested as a potential therapeutic agent for a wide range of neurological diseases. The current study aimed to evaluate the physiological changes and potential side effects after 10 weeks of 4MU treatment at a dose of 1.2 g/kg/day in healthy rats, and after 2 months of a wash-out period. Our findings revealed downregulation of hyaluronan (HA) and chondroitin sulphate proteoglycans throughout the body, significantly increased bile acids in blood samples in weeks 4 and 7 of the 4MU treatment, as well as increased blood sugars and proteins a few weeks after 4MU administration, and significantly increased interleukins IL10, IL12p70 and IFN gamma after 10 weeks of 4MU treatment. These effects, however, were reversed and no significant difference was observed between control treated and 4MU-treated animals after a 9-week wash-out period.
Gain of Aggressive Histological and Molecular Patterns after Acquired Resistance to Novel Anti-EGFR Therapies in Non-Small Cell Lung Cancer
Authors: Basse Clémence , Trabelsi-Grati Olfa , Masliah Julien , Callens Céline , Kamal Maud , Freneaux Paul , Klijanienko Jerzy , Bieche Ivan , Girard Nicolas
Published: February 2023

Novel anti-EGFR therapies target resistance to standard-of-care anti-EGFR in patients with metastatic lung cancer. We describe tumors at progression versus at the initiation of novel anti-EGFR agents in patients with metastatic lung adenocarcinoma harboring EGFR mutation. This clinical case series reports the histological and genomic features and their evolution following disease progression under amivantamab or patritumab-deruxtecan in clinical trials. All patients had a biopsy at disease progression. Four patients harboring EGFR gene mutations were included. Three of them received anterior anti-EGFR treatment. Median delay to disease progression was 15 months (range: 4–24). At progression, all tumors presented a mutation in the TP53 signaling pathway associated with a loss of heterozygosis (LOH) of the allele in 75% (n = 3), and two tumors (50%) presented an RB1 mutation associated with LOH. Ki67 expression increased above 50% (range 50–90%) in all samples compared to baseline (range 10–30%), and one tumor expressed a positive neuroendocrine marker at progression. Our work reports the potential molecular mechanisms of resistance under novel anti-EGFR in patients with metastatic EGFR-mutated lung adenocarcinoma, with the transformation to a more aggressive histology with acquired TP53 mutation and/or the increase in Ki67 expression. These characteristics are usually found in aggressive Small Cell Lung Cancer.
Polymorphisms in DNA Repair Genes and Association with Rheumatoid Arthritis in a Pilot Study on a Central European Population
Authors: Galita Grzegorz , Sarnik Joanna , Brzezinska Olga , Budlewski Tomasz , Dragan Grzegorz , Poplawska Marta , Majsterek Ireneusz , Poplawski Tomasz , Makowska Joanna S.
Published: February 2023

Rheumatoid arthritis (RA) is a chronic, multifactorial autoimmune disease characterized by chronic arthritis, a tendency to develop joint deformities, and involvement of extra-articular tissues. The risk of malignant neoplasms among patients with RA is the subject of ongoing research due to the autoimmune pathogenesis that underlies RA, the common etiology of rheumatic disease and malignancies, and the use of immunomodulatory therapy, which can alter immune system function and thus increase the risk of malignant neoplasms. This risk can also be increased by impaired DNA repair efficiency in individuals with RA, as reported in our recent study. Impaired DNA repair may reflect the variability in the genes that encode DNA repair proteins. The aim of our study was to evaluate the genetic variation in RA within the genes of the DNA damage repair system through base excision repair (BER), nucleotide excision repair (NER), and the double strand break repair system by homologous recombination (HR) and non-homologous end joining (NHEJ). We genotyped a total of 28 polymorphisms in 19 genes encoding DNA repair-related proteins in 100 age- and sex-matched RA patients and healthy subjects from Central Europe (Poland). Polymorphism genotypes were determined using the Taq-man SNP Genotyping Assay. We found an association between the RA occurrence and rs25487/XRCC1, rs7180135/RAD51, rs1801321/RAD51, rs963917/RAD51B, rs963918/RAD51B, rs2735383/NBS1, rs132774/XRCC6, rs207906/XRCC5, and rs861539/XRCC3 polymorphisms. Our results suggest that polymorphisms of DNA damage repair genes may play a role in RA pathogenesis and may be considered as potential markers of RA.
Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells
Authors: Che Hao-Yu , Zhou Chang-Hai , Lyu Chen-Chen , Meng Yu , He Yun-Tong , Wang Hao-Qi , Wu Hong-Yu , Zhang Jia-Bao , Yuan Bao
Published: February 2023

Dairy farming is the most important economic activity in animal husbandry. Mastitis is the most common disease in dairy cattle and has a significant impact on milk quality and yield. The natural extract allicin, which is the main active ingredient of the sulfur-containing organic compounds in garlic, has anti-inflammatory, anticancer, antioxidant, and antibacterial properties; however, the specific mechanism underlying its effect on mastitis in dairy cows needs to be determined. Therefore, in this study, whether allicin can reduce lipopolysaccharide (LPS)-induced inflammation in the mammary epithelium of dairy cows was investigated. A cellular model of mammary inflammation was established by pretreating bovine mammary epithelial cells (MAC-T) with 10 µg/mL LPS, and the cultures were then treated with varying concentrations of allicin (0, 1, 2.5, 5, and 7.5 µM) added to the culture medium. MAC-T cells were examined using RT–qPCR and Western blotting to determine the effect of allicin. Subsequently, the level of phosphorylated nuclear factor kappa-B (NF-κB) was measured to further explore the mechanism underlying the effect of allicin on bovine mammary epithelial cell inflammation. Treatment with 2.5 µM allicin considerably decreased the LPS-induced increase in the levels of the inflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) and inhibited activation of the NOD-like receptor protein 3 (NLRP3) inflammasome in cow mammary epithelial cells. Further research revealed that allicin also inhibited the phosphorylation of inhibitors of nuclear factor kappa-B-α (IκB-α) and NF-κB p65. In mice, LPS-induced mastitis was also ameliorated by allicin. Therefore, we hypothesize that allicin alleviated LPS-induced inflammation in the mammary epithelial cells of cows probably by affecting the TLR4/NF-κB signaling pathway. Allicin will likely become an alternative to antibiotics for the treatment of mastitis in cows.
Polyacrylamide Hydrogel Containing Calendula Extract as a Wound Healing Bandage: In Vivo Test
Authors: Ferreira Lindalva Maria de Meneses Costa , Bandeira Elanne de Sousa , Gomes Maurício Ferreira , Lynch Desireé Gyles , Bastos Gilmara Nazareth Tavares , Silva-Júnior José Otávio Carréra , Ribeiro-Costa Roseane Maria
Published: February 2023

Hydrogel is a biomaterial widely used in several areas of industry due to its great biocompatibility and adaptability to biological tissues. In Brazil, the Calendula plant is approved by the Ministry of Health as a medicinal herb. It was chosen to be incorporated in the hydrogel formulation because of its anti-inflammatory, antiseptic and healing effects. This study synthesized polyacrylamide hydrogel containing calendula extract and evaluated its efficiency as a bandage for wound healing. The hydrogels were prepared using free radical polymerization and characterized by Scanning Electron Microscopy, swelling analysis and mechanical properties by texturometer. The morphology of the matrices showed large pores and foliaceous structure. In vivo testing, as well as the evaluation of acute dermal toxicity, was conducted using male Wistar rats. The tests indicated efficient collagen fiber production, improved skin repair and no signs of dermal toxicity. Thus, the hydrogel presents compatible properties for the controlled release of calendula extract used as a bandage to promote cicatrization.
Inhibition of Xanthine Oxidase Protects against Diabetic Kidney Disease through the Amelioration of Oxidative Stress via VEGF/VEGFR Axis and NOX-FoxO3a-eNOS Signaling Pathway
Authors: Yang Keum-Jin , Choi Won Jung , Chang Yoon-Kyung , Park Cheol Whee , Kim Suk Young , Hong Yu Ah
Published: February 2023

Xanthine oxidase (XO) is an important source of reactive oxygen species. This study investigated whether XO inhibition exerts renoprotective effects by inhibiting vascular endothelial growth factor (VEGF) and NADPH oxidase (NOX) in diabetic kidney disease (DKD). Febuxostat (5 mg/kg) was administered to streptozotocin (STZ)-treated 8-week-old male C57BL/6 mice via intraperitoneal injection for 8 weeks. The cytoprotective effects, its mechanism of XO inhibition, and usage of high-glucose (HG)-treated cultured human glomerular endothelial cells (GECs) were also investigated. Serum cystatin C, urine albumin/creatinine ratio, and mesangial area expansion were significantly improved in febuxostat-treated DKD mice. Febuxostat reduced serum uric acid, kidney XO levels, and xanthine dehydrogenase levels. Febuxostat suppressed the expression of VEGF mRNA, VEGF receptor (VEGFR)1 and VEGFR3, NOX1, NOX2, and NOX4, and mRNA levels of their catalytic subunits. Febuxostat caused downregulation of Akt phosphorylation, followed by the enhancement of dephosphorylation of transcription factor forkhead box O3a (FoxO3a) and the activation of endothelial nitric oxide synthase (eNOS). In an in vitro study, the antioxidant effects of febuxostat were abolished by a blockade of VEGFR1 or VEGFR3 via NOX-FoxO3a-eNOS signaling in HG-treated cultured human GECs. XO inhibition attenuated DKD by ameliorating oxidative stress through the inhibition of the VEGF/VEGFR axis. This was associated with NOX-FoxO3a-eNOS signaling.
Evolutionary Patterns of the Chloroplast Genome in Vanilloid Orchids (Vanilloideae, Orchidaceae)
Authors: Kim Young-Kee , Cheon Se-Hwan , Hong Ja-Ram , Kim Ki-Joong
Published: February 2023

The Vanilloideae (vanilloids) is one of five subfamilies of Orchidaceae and is composed of fourteen genera and approximately 245 species. In this study, the six new chloroplast genomes (plastomes) of vanilloids (two Lecanorchis, two Pogonia, and two Vanilla species) were decoded, and then the evolutionary patterns of plastomes were compared to all available vanilloid plastomes. Pogonia japonica has the longest plastome, with 158,200 bp in genome size. In contrast, Lecanorchis japonica has the shortest plastome with 70,498 bp in genome size. The vanilloid plastomes have regular quadripartite structures, but the small single copy (SSC) region was drastically reduced. Two different tribes of Vanilloideae (Pogonieae and Vanilleae) showed different levels of SSC reductions. In addition, various gene losses were observed among the vanilloid plastomes. The photosynthetic vanilloids (Pogonia and Vanilla) showed signs of stage 1 degradation and had lost most of their ndh genes. The other three species (one Cyrotsia and two Lecanorchis), however, had stage 3 or stage 4 degradation and had lost almost all the genes in their plastomes, except for some housekeeping genes. The Vanilloideae were located between the Apostasioideae and Cypripedioideae in the maximum likelihood tree. A total of ten rearrangements were found among ten Vanilloideae plastomes when compared to the basal Apostasioideae plastomes. The four sub-regions of the single copy (SC) region shifted into an inverted repeat (IR) region, and the other four sub-regions of the IR region shifted into the SC regions. Both the synonymous (dS) and nonsynonymous (dN) substitution rates of IR in-cooperated SC sub-regions were decelerated, while the substitution rates of SC in-cooperated IR sub-regions were accelerated. A total of 20 protein-coding genes remained in mycoheterotrophic vanilloids. Almost all these protein genes show accelerated base substitution rates compared to the photosynthetic vanilloids. Two of the twenty genes in the mycoheterotrophic species faced strong “relaxed selection” pressure (p-value < 0.05).
Nanoparticle-Based Techniques for Bladder Cancer Imaging: A Review
Authors: Boschi Federico , Malatesta Manuela
Published: February 2023

Bladder cancer is very common in humans and is often characterized by recurrences, compromising the patient’s quality of life with a substantial social and economic impact. Both the diagnosis and treatment of bladder cancer are problematic due to the exceptionally impermeable barrier formed by the urothelium lining the bladder; this hinders the penetration of molecules via intravesical instillation while making it difficult to precisely label the tumor tissue for surgical resection or pharmacologic treatment. Nanotechnology has been envisaged as an opportunity to improve both the diagnostic and therapeutic approaches for bladder cancer since the nanoconstructs can cross the urothelial barrier and may be functionalized for active targeting, loaded with therapeutic agents, and visualized by different imaging techniques. In this article, we offer a selection of recent experimental applications of nanoparticle-based imaging techniques, with the aim of providing an easy and rapid technical guide for the development of nanoconstructs to specifically detect bladder cancer cells. Most of these applications are based on the well-established fluorescence imaging and magnetic resonance imaging currently used in the medical field and gave positive results on bladder cancer models in vivo, thus opening promising perspectives for the translation of preclinical results to the clinical practice.
Melatonin Alleviates Chromium Toxicity in Maize by Modulation of Cell Wall Polysaccharides Biosynthesis, Glutathione Metabolism, and Antioxidant Capacity
Authors: Yang Xiaoxiao , Ren Jianhong , Lin Xinyue , Yang Zhenping , Deng Xiping , Ke Qingbo
Published: February 2023

Melatonin, a pleiotropic regulatory molecule, is involved in the defense against heavy metal stress. Here, we used a combined transcriptomic and physiological approach to investigate the underlying mechanism of melatonin in mitigating chromium (Cr) toxicity in Zea mays L. Maize plants were treated with either melatonin (10, 25, 50 and 100 μM) or water and exposed to 100 μM K2Cr2O7 for seven days. We showed that melatonin treatment significantly decreased the Cr content in leaves. However, the Cr content in the roots was not affected by melatonin. Analyses of RNA sequencing, enzyme activities, and metabolite contents showed that melatonin affected cell wall polysaccharide biosynthesis, glutathione (GSH) metabolism, and redox homeostasis. During Cr stress, melatonin treatment increased cell wall polysaccharide contents, thereby retaining more Cr in the cell wall. Meanwhile, melatonin improved the GSH and phytochelatin contents to chelate Cr, and the chelated complexes were then transported to the vacuoles for sequestration. Furthermore, melatonin mitigated Cr-induced oxidative stress by enhancing the capacity of enzymatic and non-enzymatic antioxidants. Moreover, melatonin biosynthesis-defective mutants exhibited decreased Cr stress resistance, which was related to lower pectin, hemicellulose 1, and hemicellulose 2 than wild-type plants. These results suggest that melatonin alleviates Cr toxicity in maize by promoting Cr sequestration, re-establishing redox homeostasis, and inhibiting Cr transport from the root to the shoot.