Acceptance of Different Self-sampling Methods for Semiweekly SARS-CoV-2 Testing in Asymptomatic Children and Childcare Workers at German Day Care Centers
Authors: Engels Geraldine , Forster Johannes , Streng Andrea , Rücker Viktoria , Rudolph Paul , Pietsch Franziska , Wallstabe Julia , Wallstabe Lars , Krauthausen Maike , Schmidt Julia , Ludwig Timo , Bauer Carsten , Gierszewski David , Bendig Jesper , Timme Sandra , Jans Thomas , Weißbrich Benedikt , Romanos Marcel , Dölken Lars , Heuschmann Peter , Härtel Christoph , Gágyor Ildikó , Figge Marc Thilo , Liese Johannes , Kurzai Oliver ,   Wue-KiTa-CoV 2.0-Study Group, Amman Annsophie , Besenfelder Anastasia , Bode Leonie , Enders Inga , Frank Thiemo , Frei Kathrin , Habersack Greta , Hamway Viktoria , Höferth Theresa , Lâm Thiên-Tri , Mündlein Sabrina , Rauch Konstanze , Schoen Christoph , Spannagel Timo , Rothbauer Fabian , Abert Marianna , Bley Julia , Durnev Tatjana , Glatzle Carolin , Hardung Antonia , Karch Katharina , Kollert Sebastian , Mastorakis Tanja , Schregelmann Lena , Sokalski Victoria , Tanzberger Eva-Lotta , Thieme Anne , Yilmaz Denise , Zimmerling Stefan , Nehm Pauline , Siegl Max , Wehner Brigitte , Knies Kerstin , Richter Rebecca , Düber Hülya , Kraft Monika , Schuchardt Christian , Finkenberg Barbara , Löw Johann 
Published: September 2022

Importance

Closure of day care centers (DCCs) to contain the COVID-19 pandemic has been associated with negative effects on children’s health and well-being.

Objective

To investigate the acceptance of self-sampling methods for continuous SARS-CoV-2 surveillance among asymptomatic children and childcare workers (CCWs) in DCCs.

Design, Setting, and Participants

This nonrandomized pilot study included children and CCWs at 9 DCCs in Wuerzburg, Germany, from May to July 2021.

Interventions

Twice weekly testing for SARS-CoV-2 was conducted by self-sampled mouth-rinsing fluid (saliva sampling [SAL], with subsequent pooled polymerase chain reaction test) plus nasal rapid antigen self-test (RAgT) (group 1), SAL only (group 2), or RAgT only (group 3) in children and CCWs.

Main Outcomes and Measures

Main outcomes were rates for initial acceptance and successful (≥60% of scheduled samples) long-term participation. The probability of SARS-CoV-2 introduction into DCCs was modeled as a function of age-adjusted background incidence and DCC size.

Results

Of 836 eligible children, 452 (54.1%; 95% CI, 50.7%-57.4%) participated (median [IQR] age: 4 [3-5] years; 213 [47.1%] girls), including 215 (47.6%) in group 1, 172 (38.1%) in group 2, and 65 (14.4%) in group 3. Of 190 CCWs, 139 (73.2%; 95% CI, 66.4%-79.0%) participated (median [IQR] age: 30 [25-46] years; 128 [92.1%] women), including 96 (69.1%) in group 1, 29 (20.9%) in group 2, and 14 (10.1%) in group 3. Overall, SARS-CoV-2 PCR tests on 5306 SAL samples and 2896 RAgTs were performed in children, with 1 asymptomatic child detected by PCR from SAL. Successful long-term participation was highest in group 2 (SAL only; children: 111 of 172 [64.5%]; CCWs: 18 of 29 [62.1%]). Weekly participation rates in children ranged from 54.0% to 83.8% for SAL and from 44.6% to 61.4% for RAgT. Participation rates decreased during the study course (P &amp;lt; .001). The probability of SARS-CoV-2 introduction into a DCC with 50 children was estimated to reach at most 5% for an age-adjusted SARS-CoV-2 incidence below 143.

Conclusions and Relevance

Self-sampling for continuous SARS-CoV-2 testing was well accepted, with SAL being the preferred method. Given the high number of negative tests, thresholds for initiating continuous testing should be established based on age-adjusted SARS-CoV-2 incidence rates.

Trial Registration

German Registry for Clinical Trials Identifier: DRKS00025546