bluuebunny/crossref_metadata_2025 huggingface dataset:
Probing Downstream Olive Biophenol Secoiridoids
Authors: Sivakumar Ganapathy , Uccella Nicola A. , Gentile Luigi
Published: September 2018

Numerous bioactive biophenol secoiridoids (BPsecos) are found in the fruit, leaves, and oil of olives. These BPsecos play important roles in both the taste of food and human health. The main BPseco bioactive from green olive fruits, leaves, and table olives is oleuropein, while olive oil is rich in oleuropein downstream pathway molecules. The aim of this study was to probe olive BPseco downstream molecular pathways that are alike in biological and olive processing systems at different pHs and reaction times. The downstream molecular pathway were analyzed by high performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI/MS) and typed neglected of different overlap (TNDO) computational methods. Our study showed oleuropein highest occupied molecular orbital (HOMO) and HOMO-1 triggered the free radical processes, while HOMO-2 and lowest unoccupied molecular orbital (LUMO) were polar reactions of glucoside and ester groups. Olive BPsecos were found to be stable under acid and base catalylic experiments. Oleuropein aglycone opened to diales and rearranged to hydroxytyrosil-elenolate under strong reaction conditions. The results suggest that competition among olive BPseco HOMOs could induce glucoside hydrolysis during olive milling due to native olive β-glucosidases. The underlined olive BPsecos downstream molecular mechanism herein could provide new insights into the olive milling process to improve BPseco bioactives in olive oil and table olives, which would enhance both the functional food and the nutraceuticals that are produced from olives.
Cellular Mechanisms Responsible for Success and Failure of Bone Substitute Materials
Authors: Rolvien Tim , Barbeck Mike , Wenisch Sabine , Amling Michael , Krause Matthias
Published: September 2018

Bone grafts, i.e., autologous, allogeneic or synthetic bone substitute materials play an increasing role in reconstructive orthopedic surgery. While the indications and materials differ, it is important to understand the cellular mechanisms regarding their integration and remodeling, which are discussed in this review article. Osteoconductivity describes the new bone growth on the graft, while osteoinductivity represents the differentiation of undifferentiated cells into bone forming osteoblasts. The best case is that both mechanisms are accompanied by osteogenesis, i.e., bone modeling and remodeling of the graft material. Graft incorporation is mediated by a number of molecular pathways that signal the differentiation and activity of osteoblasts and osteoclasts (e.g., parathyroid hormone (PTH) and receptor activator of nuclear factor κβ ligand (RANKL), respectively). Direct contact of the graft and host bone as well as the presence of a mechanical load are a prerequisite for the successful function of bone grafts. Interestingly, while bone substitutes show good to excellent clinical outcomes, their histological incorporation has certain limits that are not yet completely understood. For instance, clinical studies have shown contrasting results regarding the complete or incomplete resorption and remodeling of allografts and synthetic grafts. In this context, a foreign body response can lead to complete material degradation via phagocytosis, however it may also cause a fibrotic reaction to the bone substitute. Finally, the success of bone graft incorporation is also limited by other factors, including the bone remodeling capacities of the host, the material itself (e.g., inadequate resorption, toxicity) and the surgical technique or preparation of the graft.
Inhibitory Effect of Methotrexate on Rheumatoid Arthritis Inflammation and Comprehensive Metabolomics Analysis Using Ultra-Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry (UPLC-Q/TOF-MS)
Authors: Pang Zhiqiang , Wang Guoqiang , Ran Nan , Lin Hongqiang , Wang Ziyan , Guan Xuewa , Yuan Yuze , Fang Keyong , Liu Jinping , Wang Fang
Published: September 2018

Rheumatoid arthritis (RA) is a common autoimmune disease. The inflammation in joint tissue and system endanger the human health seriously. Methotrexate have exhibited a satisfactory therapeutic effect in clinical practice. The aim of this research was to establish the pharmacological mechanism of methotrexate on RA therapy. Collagen induced arthritic rats were used to identify how methotrexate alleviates inflammation in vivo. Lipopolysaccharide-induced inflammatory proliferation in macrophages was also be detected in vitro. The activation level of Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and Nucleotide binding domain and leucine-rich repeat pyrin 3 domain (NLRP3)/Caspase-1 and related cytokines were examined by real-time PCR and western blotting or quantified with the enzyme-linked immunosorbent assay. Comprehensive metabolomics analysis was performed to identify the alteration of metabolites. Results showed that treating with methotrexate could alleviate the inflammatory condition, downregulate the activation of NF-κB and NLRP3/Caspase-1 inflammatory pathways and reduce the level of related cytokines. Docking interaction between methotrexate and caspase-1 was visualized as six H-bonds indicating a potential inhibitory effect. Metabolomics analysis reported three perturbed metabolic inflammation related pathways including arachidonic acid, linoleic acid and sphingolipid metabolism. These findings indicated that methotrexate could inhibit the onset of inflammation in joint tissue by suppressing the activation of NF-κB and NLRP3/Caspase-1 pathways and regulating the inflammation related metabolic networks.
Perioperative Serum Calcium and Phosphorus Levels are Associated with Hospital Costs and Length of Stay after Major Abdominal Surgery
Authors: Oh Tak , Jo Jihoon , Oh Ah-Young
Published: September 2018

This study aimed to identify an association between serum calcium (Ca) and phosphate (P) levels, tested during the pre- and postoperative period, with hospital costs and length of hospital stay (LOS) of patients who underwent major abdominal surgery. This retrospective cohort study analyzed the medical records of patients who underwent major abdominal surgery. A total of 3893 patients were included in the analysis, and multivariable linear regression analysis was performed. For a 1 mg/dL increase in preoperative Ca, total hospital costs decreased by 3997.9 dollars (coefficient: −3997.9, 95% confidence interval (CI): −4900.5, −30,953; p-value < 0.01), and for a 1 mg/dL increase in postoperative P, total hospital costs decreased by 702.5 dollars (coefficient: −702.5, 95% CI: −1274.5, −67.3; p-value = 0.03). Furthermore, for a 1 mg/dL increase in preoperative Ca, LOS decreased by 2.9 days (coefficient: −2.9, 95% CI: −3.7, −2.1; p-value < 0.01). For a 1 mg/dL increase in postoperative P, LOS decreased by 3.4 days (coefficient: −3.4, 95% CI: −4.2, −2.6; p-value < 0.01). This study suggested preoperative Ca and postoperative P could be useful indicators for the reduction of hospital costs and decrease in LOS from the perspective of enhanced recovery after surgery.
Axial Response of Concrete-Filled FRP Tube (CFFT) Columns with Internal Bars
Authors: Ahmed Asmaa Abdeldaim , Masmoudi Radhouane
Published: September 2018

This paper aims at investigating the general axial behavior of long circular concrete-filled, fiber-reinforced polymer (FRP) tube (CFFT) columns internally reinforced with different longitudinal rebars. A total of seven CFFT and three reinforced concrete (RC) columns served as control specimens for comparisons and were constructed and tested under cyclic axial loading until failure. The test parameters were: (1) internal reinforcement type (steel, glass fiber-reinforced polymer (GFRP) or carbon fiber-reinforced polymer (CFRP)) and amount; (2) GFRP tube thicknesses; and (3) nature of loading. All columns had 1900-mm in height and 213-mm in diameter. Examination of the test results has led to a number of significant conclusions in regards to the trend and ultimate condition of the axial stress-strain behavior, mode of failure of tested CFFT columns, and plastic strains. As expected, an increase in the FRP tube thickness (or stiffness) resulted in an increase in the strength and strain enhancement ratios. The validity of the available design provisions for predicting the ultimate load-carrying capacity of tested columns is also highlighted.
Oral Microbes, Biofilms and Their Role in Periodontal and Peri-Implant Diseases
Authors: Lasserre Jérôme Frédéric , Brecx Michel Christian , Toma Selena
Published: September 2018

Despite many discoveries over the past 20 years regarding the etio-pathogenesis of periodontal and peri-implant diseases, as well as significant advances in our understanding of microbial biofilms, the incidence of these pathologies still continues to rise. This review presents a general overview of the main protagonists and phenomena involved in oral health and disease. A special emphasis on the role of certain keystone pathogens in periodontitis and peri-implantitis is underlined. Their capacity to bring a dysregulation of the homeostasis with their host and the microbial biofilm lifestyle are also discussed. Finally, the current treatment principles of periodontitis and peri-implantitis are presented and their limits exposed. This leads to realize that new strategies must be developed and studied to overcome the shortcomings of existing approaches.
First Order Reversal Curve Study of SmFe2 Melt-Spun Ribbons
Authors: Grijalva-Castillo María C. , Santillán-Rodríguez Carlos R. , Sáenz-Hernández Renee J. , Botello-Zubíate María E. , Matutes-Aquino José A.
Published: September 2018

First-order reversal curves (FORC) and the FORC distribution provide a detailed characterization of the relative proportions of reversible and irreversible components of the magnetization of a material, revealing the dominant interactions in the system. Alloys with the nominal composition SmFe2 were obtained by melt-spinning with a cooper wheel velocity of 30 m/s. X-ray powder diffraction analysis showed a greater part consisting of an amorphous phase and a very small amount of SmFe2 crystalline phase with an average crystallite size of 8 nm. A constant acceleration Mössbauer spectrum, measured at room temperature in transmission mode, was fitted to a continuous distribution of effective fields at the nucleus of the amorphous phase (about 84% of the total area), plus two sextets for the non-equivalent sites of Fe in the SmFe2 crystalline phase. 91 first-order reversal curves were collected in a Quantum Design PPMS-VSM with reversal fields from –800 mT to +800 mT and using a calibration field of 850 mT. The obtained FORC diagrams showed a combined effect of a local interaction field and a mean interaction field, and showed that the reversible magnetization is a function of both, the applied magnetic field and the irreversible magnetization.
Effects of Pr and Yb Dual Doping on the Thermoelectric Properties of CaMnO3
Authors: Li Cuiqin , Chen Qianlin , Yan Yunan
Published: September 2018

There has been research on CaMnO3 with natural abundance, low toxicity, and low cost as promising candidates for n-type thermoelectric (TE) materials. In this paper, Ca1−2xPrxYbxMnO3 with different Pr and Yb contents (x = 0, 0.01, 0.02, 0.03, 0.04 and 0.05) were synthesized by means of coprecipitation. With X-ray diffraction (XRD), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM), researchers characterized the phase structure and morphology of all the samples. The oxidation states of manganese were determined by X-ray photoemission spectroscopy (XPS). The role of Ca-site dual doping in the TE properties was also investigated. Increasing the Pr and Yb contents leads to decreases in the electrical resistivity and Seebeck coefficient, leading to a power factor of 3.48 × 10−4 W·m−1·K−2 for x = 0.04 at 773 K, which is its maximum. Furthermore, the thermal conductivity (κ) decreases with increasing x, and κ = 1.26 W·m−1·K−1 is obtained for x = 0.04 at 973 K. Ca0.92Pr0.04Yb0.04MnO3 exhibit a ZT (thermoelectric figure of merit) value of 0.24 at 973 K, approximately 3 times more than that of the pristine CaMnO3. Thus, the reported method is a new strategy to enhance the TE performance of CaMnO3.
Corrosion Behavior of the As-Cast and As-Solid Solution Mg-Al-Ge Alloy
Authors: Liu Xiaoda , Yin Ming , Zhang Shaohua , Wei Huan , Liu Baosheng , Du Huayun , Hou Lifeng , Wei Yinghui
Published: September 2018

The corrosion behavior of Mg-3Al-xGe (x = 1, 3, 5) alloy in as-cast and as-solid was investigated by virtue of microstructure, corrosion morphology observation, and electrochemical measurement. Among the as-cast alloys, the corrosion rate of Mg-3Al-1Ge with a discontinuous bar-morphology was the highest, which was 101.7 mm·a−1; the corrosion rate of Mg-3Al-3Ge with a continuous network distribution was the lowest, which was 23.1 mm·a−1; and the corrosion rate of Mg-3Al-5Ge of Ge-enriched phase with sporadic distribution was in-between, which was 63.9 mm·a−1. It is suggested that the morphology of the Mg2Ge phase changes with a change in Ge content, which affects the corrosion performance of the alloy. After solid solution treatment, the corrosion rate of the corresponding solid solution alloy increased—Mg-3Al-1Ge to 140.5 mm·a−1, Mg-3Al-3Ge to 52.9 mm·a−1, and Mg-3Al-5Ge to 87.3 mm·a−1, respectively. After investigation of the microstructure, it can be suggested that solid solution treatment dissolves the Mg17Al12 phase, which changes the phase composition of the alloy and also affects its microstructure, thus affecting its corrosion performance.
A Review on the Use of Hydroxyapatite-Carbonaceous Structure Composites in Bone Replacement Materials for Strengthening Purposes
Authors: Siddiqui Humair A. , Pickering Kim L. , Mucalo Michael R.
Published: September 2018

Biomedical materials constitute a vast scientific research field, which is devoted to producing medical devices which aid in enhancing human life. In this field, there is an enormous demand for long-lasting implants and bone substitutes that avoid rejection issues whilst providing favourable bioactivity, osteoconductivity and robust mechanical properties. Hydroxyapatite (HAp)-based biomaterials possess a close chemical resemblance to the mineral phase of bone, which give rise to their excellent biocompatibility, so allowing for them to serve the purpose of a bone-substituting and osteoconductive scaffold. The biodegradability of HAp is low (Ksp ≈ 6.62 × 10−126) as compared to other calcium phosphates materials, however they are known for their ability to develop bone-like apatite coatings on their surface for enhanced bone bonding. Despite its favourable bone regeneration properties, restrictions on the use of pure HAp ceramics in high load-bearing applications exist due to its inherently low mechanical properties (including low strength and fracture toughness, and poor wear resistance). Recent innovations in the field of bio-composites and nanoscience have reignited the investigation of utilising different carbonaceous materials for enhancing the mechanical properties of composites, including HAp-based bio-composites. Researchers have preferred carbonaceous materials with hydroxyapatite due to their inherent biocompatibility and good structural properties. It has been demonstrated that different structures of carbonaceous material can be used to improve the fracture toughness of HAp, as they can easily serve the purpose of being a second phase reinforcement, with the resulting composite still being a biocompatible material. Nanostructured carbonaceous structures, especially those in the form of fibres and sheets, were found to be very effective in increasing the fracture toughness values of HAp. Minor addition of CNTs (3 wt.%) has resulted in a more than 200% increase in fracture toughness of hydroxyapatite-nanorods/CNTs made using spark plasma sintering. This paper presents a current review of the research field of using different carbonaceous materials composited with hydroxyapatite with the intent being to produce high performance biomedically targeted materials.
Characterization and Antioxidant and Angiotensin I-Converting Enzyme (ACE)-Inhibitory Activities of Gelatin Hydrolysates Prepared from Extrusion-Pretreated Milkfish (Chanos chanos) Scale
Authors: Huang Chun-Yung , Tsai Yung-Hsiang , Hong Yong-Han , Hsieh Shu-Ling , Huang Ren-Han
Published: September 2018

Fish gelatin hydrolysates have been shown to possess various biological activities due to their unique Gly-Pro-Y and Gly-X-Hyp sequences. In the current study, fish gelatin was extracted from non-extruded milkfish scale (FSG1) or extrusion-pretreated milkfish scale (FSG2); extracted gelatins were hydrolyzed with different combinations of Flavourzyme and Alcalase to give four different hydrolysates, namely: FSGH1 (FSG1 hydrolyzed with Flavourzyme), FSGH2 (FSG1 hydrolyzed with Alcalase + Flavourzyme), FSGH3 (FSG2 hydrolyzed with Flavourzyme), and FSGH4 (FSG2 hydrolyzed with Alcalase + Flavourzyme). The extrusion-pretreatment process enhanced the extraction yield of gelatin from fish scale. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared (FTIR) analyses showed the extracts FSG1 and FSG2 possessed characteristics of gelatin. Moreover, the physicochemical characteristics of FSGH1–FSGH4 were examined by analyses of their degree of hydrolysis, amino acid composition, UV spectrum, FTIR spectrum, molecular weight, and RP-HPLC profile. Additional biological functional analyses showed that all of the studied gelatin hydrolysates FSGH1–FSGH4 possessed antioxidant activity dose-dependently as revealed by DPPH scavenging, ABTS scavenging, and reducing power analyses. In addition, FSGH2 and FSGH4 showed higher angiotensin-I-converting enzyme (ACE)-inhibitory activity as compared to FSGH1 and FSGH3. Taken together, FSGH2 and FSGH4 showed high antioxidant activity and potent anti-ACE activity. Due to the potential antioxidant and antihypertensive properties of FSGH2 and FSGH4, further research is needed to explore their possible use as natural supplementary raw materials in food and nutraceutical products.
Characterization of Properties and Transglycosylation Abilities of Recombinant α-Galactosidase from Cold-Adapted Marine Bacterium Pseudoalteromonas KMM 701 and Its C494N and D451A Mutants
Authors: Bakunina Irina , Slepchenko Lubov , Anastyuk Stanislav , Isakov Vladimir , Likhatskaya Galina , Kim Natalya , Tekutyeva Liudmila , Son Oksana , Balabanova Larissa
Published: September 2018

A novel wild-type recombinant cold-active α-d-galactosidase (α-PsGal) from the cold-adapted marine bacterium Pseudoalteromonas sp. KMM 701, and its mutants D451A and C494N, were studied in terms of their structural, physicochemical, and catalytic properties. Homology models of the three-dimensional α-PsGal structure, its active center, and complexes with D-galactose were constructed for identification of functionally important amino acid residues in the active site of the enzyme, using the crystal structure of the α-galactosidase from Lactobacillus acidophilus as a template. The circular dichroism spectra of the wild α-PsGal and mutant C494N were approximately identical. The C494N mutation decreased the efficiency of retaining the affinity of the enzyme to standard p-nitrophenyl-α-galactopiranoside (pNP-α-Gal). Thin-layer chromatography, matrix-assisted laser desorption/ionization mass spectrometry, and nuclear magnetic resonance spectroscopy methods were used to identify transglycosylation products in reaction mixtures. α-PsGal possessed a narrow acceptor specificity. Fructose, xylose, fucose, and glucose were inactive as acceptors in the transglycosylation reaction. α-PsGal synthesized -α(1→6)- and -α(1→4)-linked galactobiosides from melibiose as well as -α(1→6)- and -α(1→3)-linked p-nitrophenyl-digalactosides (Gal2-pNP) from pNP-α-Gal. The D451A mutation in the active center completely inactivated the enzyme. However, the substitution of C494N discontinued the Gal-α(1→3)-Gal-pNP synthesis and increased the Gal-α(1→4)-Gal yield compared to Gal-α(1→6)-Gal-pNP.
Influence of Thermal Aging Parameters on the Characteristics of Aluminum Semi-Solid Alloys
Authors: Ragab Khaled Ahmed , Bouazara Mohamed , Chen Xiao Grant
Published: September 2018

The current study aimed at analyzing the response of semisolid A357 aluminum alloys to unconventional thermal treatment cycles of T4/T6/T7 conditions. The mechanical, electrical, and microstructural characterizations of such semisolid alloys were investigated. The microstructure evolutions of Fe-intermetallic phases and strengthening precipitates were characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. The mechanical failure of such semi solid A357 aluminum alloys, used for suspension automotive parts, is mostly related to cracking issues which start from the surface due to hardness problems and propagate due to severe load variations. For these reasons, the multiple thermal aging cycles, in this study, are applied to enhance the mechanical properties and to have compromised values compared to those obtained by standard thermal treatments. The results obtained in this work indicate that the heat treatment of this alloy can be optimized. The results showed that the optimum characteristics of A357 semisolid alloys were obtained by applying thermal under-aging cycle, interrupted thermal aging cycles and a T7/T6 two steps aging treatment condition. The electrical conductivity and electron microscopy were applied in this study to analyze the characteristics of hardening phases formed due to different aging cycles applied to the alloys investigated.
Effect of Carbon Partitioning, Carbide Precipitation, and Grain Size on Brittle Fracture of Ultra-High-Strength, Low-Carbon Steel after Welding by a Quenching and Partitioning Process
Authors: Forouzan Farnoosh , Guitar M. Agustina , Vuorinen Esa , Mücklich Frank
Published: September 2018

To improve the weld zone properties of Advanced High Strength Steel (AHSS), quenching and partitioning (Q&P) has been used immediately after laser welding of a low-carbon steel. However, the mechanical properties can be affected for several reasons: (i) The carbon content and amount of retained austenite, bainite, and fresh martensite; (ii) Precipitate size and distribution; (iii) Grain size. In this work, carbon movements during the partitioning stage and prediction of Ti (C, N), and MoC precipitation at different partitioning temperatures have been simulated by using Thermocalc, Dictra, and TC-PRISMA. Verification and comparison of the experimental results were performed by optical microscopy, X-ray diffraction (XRD), Scanning Electron Microscop (SEM), and Scanning Transmission Electron Microscopy (STEM), and Energy Dispersive Spectroscopy (EDS) and Electron Backscatter Scanning Diffraction (EBSD) analysis were used to investigate the effect of martensitic/bainitic packet size. Results show that the increase in the number density of small precipitates in the sample partitioned at 640 °C compensates for the increase in crystallographic packets size. The strength and ductility values are kept at a high level, but the impact toughness will decrease considerably.
Effect of T6 Heat Treatment on the Microstructure and Hardness of Secondary AlSi9Cu3(Fe) Alloys Produced by Semi-Solid SEED Process
Authors: Fabrizi Alberto , Capuzzi Stefano , De Mori Alessandro , Timelli Giulio
Published: September 2018

The effect of the T6 heat treatment on the microstructure and hardness of a secondary semi-solid AlSi9Cu3(Fe) alloy have been investigated by using optical, scanning and transmission electron microscopy and hardness testing. The semi-solid alloy was produced using the swirled enthalpy equilibration device (SEED). The solution heat treatments were performed at 450, 470 and 490 °C for 1 to 6 h followed by water quenching and artificial ageing at 160, 180 and 220 °C for holding times ranging from 1 to 30 h. The microstructural investigations have revealed the spheroidization of the eutectic Si and the dissolution of the majority of Cu-rich compounds after all the solution heat treatments; moreover, the greater the solution temperature and time, the higher the hardness of the alloy. Unacceptable surface blistering has been observed for severe solution condition, 490 °C for 3 and 6 h. The artificial ageing at 160 °C for 24 h has led to the highest alloy strengthening thanks to the precipitation of β” and Q’ (or L) phases within the α-Al matrix. The hardening peaks at higher temperatures have been early achieved due to faster hardening kinetic; however, the lower number density of β” and Q’ (or L) phases and the presence of coarser θ’ precipitates result in a reduction of hardness values for peak aged condition at 180 and 220 °C, respectively.
The Microstructure and Deformation Behavior of Al-Fe-Mn Alloys with Different Fe Contents during Cold Rolling
Authors: Pan Yanfeng , Shen Yifu , Zhao Pizhi
Published: September 2018

The microstructure transformations and deformation behavior of Al-Fe-Mn alloys with different Fe contents during their cold rolling process were investigated by means of hardness testing, conductivity testing, and transmission electron microscopy. It was observed that the hardness of the two alloys increased initially along with the levels of cold rolling reduction, then reduced when levels of cold rolling reduction increased further. Two kinds of deformation behaviors, work hardening and work softening, were observed during cold rolling for both Al-Fe-Mn alloys with different Fe contents. The critical level of cold rolling reduction that led to the change from work hardening to work softening was different in both alloys and the critical level of cold rolling reduction of the alloy with high Fe content was significantly lower than that of the alloy with low Fe content. During the work hardening process, the number of dislocations in the alloys increased continuously as the level of cold rolling reduction increased and they were accompanied by the formation of substructures. After the occurrence of work softening, the dislocation density in the alloys was significantly reduced. The sub-grain structures polygonized and ultimately transformed into equiaxed sub-grains.
Crystallization Behaviors of Anosovite and Silicate Crystals in High CaO and MgO Titanium Slag
Authors: Fan Helin , Chen Dengfu , Liu Tao , Duan Huamei , Huang Yunwei , Long Mujun , He Wenjie
Published: September 2018

Electric-furnace smelting has become the dominant process for the production of the titanium slag from ilmenite in China. The crystallization behaviors of anosovite and silicate crystals in the high CaO and MgO titanium slag were studied to insure smooth operation of the smelting process and the efficient separation of titanium slag and metallic iron. The crystallization behaviors were studied by a mathematical model established in this work. Results show that the crystallization order of anosovite and silicate crystals in high CaO and MgO titanium slag during cooling is: Al2TiO5 > Ti3O5 > MgTi2O5 > MgSiO3 > CaSiO3 > FeTi2O5 > Mn2SiO4 > Fe2SiO4. Al2TiO5 and Ti3O5 have higher crystallization priority and should be responsible for the sharp increase in viscosity of titanium slag during cooling. The total crystallization rates of anosovite and silicate crystals are mainly controlled by Al2TiO5 and MgSiO3, respectively. The mass ratio of Ti2O3/ΣTiO2 has a prominent influence on the total crystallization rate of anosovite crystals while the mass ratio of MgO/FeO has a slight influence on the total crystallization rate of anosovite crystals.
Evaluation of Electrovibration Stimulation with a Narrow Electrode
Authors: Ishizuka Hiroki , Komurasaki Seiya , Kato Kunihiro , Kajimoto Hiroyuki
Published: September 2018

Recently, electrovibration tactile displays were studied and applied to several use cases by researchers. The high-resolution electrode for electrovibration stimulus will contribute to the presentation of a more realistic tactile sensation. However, the sizes of the electrodes that have been used thus far are of the millimeter-order. In this study, we evaluated whether a single narrow electrode was able to provide the electrovibration stimulus adequately. The widths of the prepared electrodes were 10, 20, 50, 100, 200, and 500 μm. We conducted a sensory experiment to characterize each electrode. The electrodes with widths of 50 μm or less were not durable or suitable for the applied signal, although the subjects perceived the stimulus. Therefore, we conducted the experiment without using these non-durable electrodes. The voltage waveform condition affected perception, and the subjects were not sensitive to the electrovibration stimulus at low frequencies. In addition, the stroke direction of the fingertip had a significant effect on perception under certain conditions. The results indicate that electrovibration stimulation requires an electrode with a width of only a few hundred micrometers for stimulation.
HS-SPME-GC-MS Analyses of Volatiles in Plant Populations—Quantitating Compound × Individual Matrix Effects
Authors: Burzynski-Chang Elizabeth A. , Ryona Imelda , Reisch Bruce I. , Gonda Itay , Foolad Majid R. , Giovannoni James J. , Sacks Gavin L.
Published: September 2018

Headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography–mass spectrometry (GC-MS) is widely employed for volatile analyses of plants, including mapping populations used in plant breeding research. Studies often employ a single internal surrogate standard, even when multiple analytes are measured, with the assumption that any relative changes in matrix effects among individuals would be similar for all compounds, i.e., matrix effects do not show Compound × Individual interactions. We tested this assumption using individuals from two plant populations: an interspecific grape (Vitis spp.) mapping population (n = 140) and a tomato (Solanum spp.) recombinant inbred line (RIL) population (n = 148). Individual plants from the two populations were spiked with a cocktail of internal standards (n = 6, 9, respectively) prior to HS-SPME-GC-MS. Variation in the relative responses of internal standards indicated that Compound × Individual interactions exist but were different between the two populations. For the grape population, relative responses among pairs of internal standards varied considerably among individuals, with a maximum of 249% relative standard deviation (RSD) for the pair of [U13C]hexanal and [U13C]hexanol. However, in the tomato population, relative responses of internal standard pairs varied much less, with pairwise RSDs ranging from 8% to 56%. The approach described in this paper could be used to evaluate the suitability of using surrogate standards for HS-SPME-GC-MS studies in other plant populations.
Metallic Nanoparticles: General Research Approaches to Immunological Characterization
Authors: Gatto Francesca , Bardi Giuseppe
Published: September 2018

Our immunity is guaranteed by a complex system that includes specialized cells and active molecules working in a spatially and temporally coordinated manner. Interaction of nanomaterials with the immune system and their potential immunotoxicity are key aspects for an exhaustive biological characterization. Several assays can be used to unravel the immunological features of nanoparticles, each one giving information on specific pathways leading to immune activation or immune suppression. Size, shape, and surface chemistry determine the surrounding corona, mainly formed by soluble proteins, hence, the biological identity of nanoparticles released in cell culture conditions or in a living organism. Here, we review the main laboratory characterization steps and immunological approaches that can be used to understand and predict the responses of the immune system to frequently utilized metallic or metal-containing nanoparticles, in view of their potential uses in diagnostics and selected therapeutic treatments.
Role of Chemosensory TRP Channels in Lung Cancer
Authors: Büch Thomas R. H. , Büch Eva A. M. , Boekhoff Ingrid , Steinritz Dirk , Aigner Achim
Published: September 2018

Transient receptor potential (TRP) channels represent a large family of cation channels and many members of the TRP family have been shown to act as polymodal receptor molecules for irritative or potentially harmful substances. These chemosensory TRP channels have been extensively characterized in primary sensory and neuronal cells. However, in recent years the functional expression of these proteins in non-neuronal cells, e.g., in the epithelial lining of the respiratory tract has been confirmed. Notably, these proteins have also been described in a number of cancer types. As sensor molecules for noxious compounds, chemosensory TRP channels are involved in cell defense mechanisms and influence cell survival following exposure to toxic substances via the modulation of apoptotic signaling. Of note, a number of cytostatic drugs or drug metabolites can activate these TRP channels, which could affect the therapeutic efficacy of these cytostatics. Moreover, toxic inhalational substances with potential involvement in lung carcinogenesis are well established TRP activators. In this review, we present a synopsis of data on the expression of chemosensory TRP channels in lung cancer cells and describe TRP agonists and TRP-dependent signaling pathways with potential relevance to tumor biology. Furthermore, we discuss a possible role of TRP channels in the non-genomic, tumor-promoting effects of inhalational carcinogens such as cigarette smoke.
A Novel PAA Derivative with Enhanced Drug Efficacy in Pancreatic Cancer Cell Lines
Authors: Alsuraifi Ali , Lin Paul Kong Thoo , Curtis Anthony , Lamprou Dimitrios A. , Hoskins Clare
Published: September 2018

Nanoparticles have been shown to be effective drug carriers in cancer therapy. Pancreatic cancer forms dense tumours which are often resistant to drug molecules. In order to overcome such multidrug resistance, new drug entities, novel delivery systems and combination therapy strategies are being explored. In this paper, we report the design and synthesis of a poly(allylamine)-based amphiphile modified with hydrophobic naphthalimido pendant groups. Bisnaphthalimide compounds have been shown to possess anticancer activity. The potential of this polymer to encapsulate, solubilize and enhance drug (5-fluorouricil and bis-(naphthalimidopropyl)-diaminooctane) cytotoxicity in BxPC-3 cells was evaluated. Our studies showed that the insoluble drugs could be formulated up to 4.3 mg mL−1 and 2.4 mg mL−1 inside the amphiphiles, respectively. Additionally, the novel poly(allylamine)-naphthalimide carrier resulted in an amplification of cytotoxic effect with drug treatment after 24 h, and was capable of reduction of 50% cell population at concentrations as low as 3 μg mL−1.
The Electrical, Mechanical and Surface Properties of Thermoplastic Polyester Elastomer Modified by Electron Beta Radiation
Authors: Manas David , Mizera Ales , Navratil Milan , Manas Miroslav , Ovsik Martin , Sehnalek Stanislav , Stoklasek Pavel
Published: September 2018

The main advantages of Thermoplastic Polyester Elastomers (TPE-E) are their elastomer properties as well as their ability to be processed in the same way as thermoplastic polymers (e.g., injection moulding, compression moulding and extrusion). However, TPE-Es’ properties, mainly their mechanical properties and thermal characteristics, are not as good as those of elastomers. Because of this TPE-Es are often modified with the aim of improving their properties and extending their range of application. Radiation cross-linking using accelerated electron beams is one of the most effective ways to change virgin polymers’ properties significantly. Their electrical (that is to say permittivity and resistivity measurements), mechanical (that is, tensile and impact tensile tests), as well as surface (that is, nano-indentation) properties were measured on modified/cross-linked TPE-E specimens with and/or without a cross-linking agent at irradiation doses of 0, 33, 66, 99, 132, 165 and 198 kGy. The data acquired from these procedures show significant changes in the measured properties. The results of this study allow the possibility of determining the proper processing parameters and irradiation doses for the production of TPE-E products which leads to the enlargement of their application in practice.
Cellulose–Chitosan Antibacterial Composite Films Prepared from LiBr Solution
Authors: Yang Jiwook , Kwon Gu-Joong , Hwang Kyojung , Kim Dae-Young
Published: September 2018

Cellulose and chitosan solutions were prepared in 60% LiBr and mixed with a different weight ratio. The washing and drying of the prepared cellulose–chitosan composite films were performed under identical conditions. The color of the liquefied mixtures and films was initially transparent but changed from colorless to brownish yellow depending on the ratio of chitosan in the solution. The cross section observed in the SEM results indicated that the film developed with a higher ratio of chitosan was more robust and possessed greater antibacterial properties. FT-IR analysis of the films showed that hydrogen bonds between cellulose and chitosan in composite films were successfully achieved and retained excellent mechanical properties. The proper ratio of chitosan in the cellulose solution can increase the tensile strength and improve the elongation of the films; however, the E-modulus property was consistently reduced. The antibacterial activity and mechanical properties of the films were greatly improved as the amount of chitosan in the film increased.
Paternity Leave, Father Involvement, and Parental Conflict: The Moderating Role of Religious Participation
Authors: Petts Richard J.
Published: September 2018

Numerous studies show that taking paternity leave is associated with increased father involvement. However, fewer studies have explored contextual factors that may increase (or diminish) the likelihood that paternity leave-taking provides benefits to families. Using data from the Fragile Families and Child Wellbeing Study, this study examines the associations between paternity leave, fathers’ religious participation, father involvement, and parental conflict, and whether fathers’ religious participation moderates the associations between paternity leave, father involvement, and parental conflict. Results suggest that paternity leave-taking, length of paternity leave, and fathers’ religious participation are associated with increased father involvement but are unrelated to parental conflict. Results also suggest that religious participation may enhance the association between paternity leave and family outcomes; paternity leave-taking and length of paternity leave are only associated with lower levels of parental conflict among families in which fathers attend religious services frequently. Moreover, fathers who take leave and attend religious services frequently are more likely to be involved with their child than fathers who take leave but do not attend religious services.
Using Satellite Remote Sensing to Study the Impact of Climate and Anthropogenic Changes in the Mesopotamian Marshlands, Iraq
Authors: Albarakat Reyadh , Lakshmi Venkat , Tucker Compton J.
Published: September 2018

The Iraqi Marshes in Southern Iraq are considered one of the most important wetlands in the world. From 1982 to the present, their area has varied between 10,500 km2 and 20,000 km2. The marshes support a variety of plants, such as reeds and papyrus, and are home to many species of birds. These marshes are Al-Hammar, Central or Al-Amarah, and Al-Huwaiza. Freshwater supplies to the marshes come from the Tigris and Euphrates rivers in Iraq and from the Karkha River from Iran. For this analysis, we used the Land Long-Term Data Record Version 5 (LTDR V5) Normalized Difference Vegetation Index (NDVI) from the Advanced Very High Resolution Radiometer (AVHRR) sensor dataset. This dataset was recently released at a 0.05 × 0.05° spatial resolution and daily temporal resolution to monitor the spatial and temporal variability of vegetation along with other hydrological variables such as land surface temperature, precipitation, and evapotranspiration. In our analysis, we considered three time periods: 1982–1992; 1993–2003; and 2004–2017 due to anthropogenic activities and climate changes. Furthermore, we examined the relationships between various water cycle variables through the investigation of vegetation and water coverage changes, and studied the impacts of climate change and anthropogenic activities on the Iraqi Marshes and considered additional ground observations along with the satellite datasets. Statistical analyses over the last 36 years show significant deterioration in the vegetation: 68.78%, 98.73, and 83.71% of the green biomass has declined for Al-Hammar, The Central marshes, and Al-Huwaiza, respectively. The AVHRR and Landsat images illustrate a decrease in water and vegetation coverage, which in turn has led to an increase in barren lands. Unfortunately, statistical analyses show that marshland degradation is mainly induced by human actions. The shrinkage in water supplies taken by Iraq’s local neighbors (i.e., Turkey, Syria, and Iran) has had a sharp impact on water levels. The annual discharge of the Tigris declined from ~2500–3000 m3/s to ~500 m3/s, and the annual discharge of the Euphrates River declined from ~1500 m3/s to less than 500 m3/s.
Spatiotemporal Patterns of Vegetation Greenness Change and Associated Climatic and Anthropogenic Drivers on the Tibetan Plateau during 2000–2015
Authors: Li Lanhui , Zhang Yili , Liu Linshan , Wu Jianshuang , Wang Zhaofeng , Li Shicheng , Zhang Huamin , Zu Jiaxing , Ding Mingjun , Paudel Basanta
Published: September 2018

Alpine vegetation on the Tibetan Plateau (TP) is known to be sensitive to both climate change and anthropogenic disturbance. However, the magnitude and patterns of alpine vegetation dynamics and the driving mechanisms behind their variation on the TP remains under debate. In this study, we used updated MODIS Collection 6 Normalized Difference Vegetation Index (NDVI) from the Terra satellite combined with linear regression and the Break for Additive Season and Trend model to reanalyze the spatiotemporal patterns of vegetation change on the TP during 2000–2015. We then quantified the responses of vegetation variation to climatic and anthropogenic factors by coupling climatic and human footprint datasets. Results show that growing season NDVI (GNDVI) values increased significantly overall (0.0011 year−1, p < 0.01) during 2000–2015 and that 70.37% of vegetated area on the TP (23.47% significantly with p < 0.05) exhibited greening trends with the exception of the southwest TP. However, vegetation greenness experienced trend shifts from greening to browning in half of the ecosystem zones occurred around 2010, likely induced by spatially heterogeneous temporal trends of climate variables. The vegetation changes in the northeastern and southwestern TP were water limited, the mid-eastern TP exhibited strong temperature responses, and the south of TP was driven by a combination of temperature and solar radiation. Furthermore, we found that, to some extent, anthropogenic disturbances offset climate-driven vegetation greening and aggravated vegetation browning induced by water deficit. These findings suggest that the impact of anthropogenic activities on vegetation change might not overwhelm that of climate change at the region scale.
Landslide Detection and Susceptibility Mapping by AIRSAR Data Using Support Vector Machine and Index of Entropy Models in Cameron Highlands, Malaysia
Authors: Tien Bui Dieu , Shahabi Himan , Shirzadi Ataollah , Chapi Kamran , Alizadeh Mohsen , Chen Wei , Mohammadi Ayub , Ahmad Baharin , Panahi Mahdi , Hong Haoyuan , Tian Yingying
Published: September 2018

Since landslide detection using the combination of AIRSAR data and GIS-based susceptibility mapping has been rarely conducted in tropical environments, the aim of this study is to compare and validate support vector machine (SVM) and index of entropy (IOE) methods for landslide susceptibility assessment in Cameron Highlands area, Malaysia. For this purpose, ten conditioning factors and observed landslides were detected by AIRSAR data, WorldView-1 and SPOT 5 satellite images. A spatial database was generated including a total of 92 landslide locations encompassing the same number of observed and detected landslides, which was divided into training (80%; 74 landslide locations) and validation (20%; 18 landslide locations) datasets. Results of the difference between observed and detected landslides using root mean square error (RMSE) indicated that only 16.3% error exists, which is fairly acceptable. The validation process was performed using statistical-based measures and the area under the receiver operating characteristic (AUROC) curves. Results of validation process indicated that the SVM model has the highest values of sensitivity (88.9%), specificity (77.8%), accuracy (83.3%), Kappa (0.663) and AUROC (84.5%), followed by the IOE model. Overall, the SVM model applied to detected landslides is considered to be a promising technique that could be tested and utilized for landslide susceptibility assessment in tropical environments.
Quantitative Identification of Maize Lodging-Causing Feature Factors Using Unmanned Aerial Vehicle Images and a Nomogram Computation
Authors: Han Liang , Yang Guijun , Feng Haikuan , Zhou Chengquan , Yang Hao , Xu Bo , Li Zhenhai , Yang Xiaodong
Published: September 2018

Maize (zee mays L.) is one of the most important grain crops in China. Lodging is a natural disaster that can cause significant yield losses and threaten food security. Lodging identification and analysis contributes to evaluate disaster losses and cultivates lodging-resistant maize varieties. In this study, we collected visible and multispectral images with an unmanned aerial vehicle (UAV), and introduce a comprehensive methodology and workflow to extract lodging features from UAV imagery. We use statistical methods to screen several potential feature factors (e.g., texture, canopy structure, spectral characteristics, and terrain), and construct two nomograms (i.e., Model-1 and Model-2) with better validation performance based on selected feature factors. Model-2 was superior to Model-1 in term of its discrimination ability, but had an over-fitting phenomenon when the predicted probability of lodging went from 0.2 to 0.4. The results show that the nomogram could not only predict the occurrence probability of lodging, but also explore the underlying association between maize lodging and the selected feature factors. Compared with spectral features, terrain features, texture features, canopy cover, and genetic background, canopy structural features were more conclusive in discriminating whether maize lodging occurs at the plot scale. Using nomogram analysis, we identified protective factors (i.e., normalized difference vegetation index, NDVI and canopy elevation relief ratio, CRR) and risk factors (i.e., Hcv) related to maize lodging, and also found a problem of terrain spatial variability that is easily overlooked in lodging-resistant breeding trials.