bluuebunny/crossref_metadata_2025 huggingface dataset:
Astigmatism correction and corneal aberrations variability between two locations of cap side-cut after small incision lenticule extraction: a randomized study
Authors: Zhang Ming , Yang Wen , Yang Junfeng , Guo Juan , Shu Zhiyu , Song Lei , Chen Ling , Jiang Yanliang
Published: March 2022

Purpose To investigate astigmatism correction and the effect on corneal aberrations between two locations of the cap side-cut (CSC) after small incision lenticule extraction(SMILE) surgery.Methods In this randomized, prospective research, 91 eyes of 49 patients were investigated after SMILE. The eyes were randomized into two groups with different CSC locations. The location was set at 120° for group 1 and 90° for group 2. Uncorrected distant vision acuity (UDVA) and corrected distant vision acuity measurements, manifest refraction, and imaging were performed before and after surgery.Results At the 3-month follow-up, all eyes in both groups had a postoperative UDVA ≥ 20/20. There was no significantly difference in the refraction data nor in the safety and efficacy indices between the two groups (P=0.343 and P=0.738, separately) . The effects on corneal astigmatism, corneal root mean square high-order aberration, corneal spherical aberration, and corneal coma aberration at 0º and 90º showed no difference between the two groups.Conclusions Astigmatic correction was not influenced by two locations of the CSC in SMILE surgery. No significant difference was found in visual and corneal optical quality results with two different CSCs in SMILE surgery.
Oxidative degradation of Metolcarb by electro Fenton process
Authors: Bakhti Hayet , Hamida Najib Ben , Hauchard Didier
Published: March 2022

This study presents the degradation and the mineralization of Metolcarb or 3-methylphenyl methylcarbamate by electro Fenton process with volume cathode. This pesticide was treated using this cathode which is filled with graphite carbon granules. The solutions of Metolcarb were electrolyzed in an reactor of 1.4 liter which shows the performance and the efficiency of the process. Indeed, the effects of different parameters such as the initial concentration of Metolcarb and the intensity of applied current on the degradation kinetics of Metolcarb have been discussed as a function of time. The found results proved that the insecticide removal followed a pseudo first order. The competitive kinetics analysis of Metolcarb during its electrochemical treatment was carried out by using benzoic acid as a reference compound. The absolute rate constant for the oxidation of Metolcarb by hydroxyl radicals consequently is equal to 3.59 × 109 L mol− 1 s− 1. The mineralization of Metolcarb in terms of total organic carbon was monitored during its electrolysis by the electro Fenton process. The organic carbon is then equivalent to the total carbon determined in reaction analysis. Carbon chains can therefore be formed. The various intermediates which are produced into the electrochemical processing of Metolcarb were determined by the mass spectrometry LC/MS. The biodegradation of Metolcarb has also been investigated in active clay.
Osseointegration Reduces Aseptic Loosening of Primary Distal Femoral Implants in Pediatric and Adolescent Osteosarcoma Patients: A Retrospective Clinical and Radiographic Study
Authors: Welsh Clayton , Hull Peyton , Meckmongkol Teerin , Mumith Aadil , Lovejoy John , Giangarra Charles , Coathup Melanie
Published: March 2022

AimThe challenge of distal femoral replacement (DFR) longevity remains a priority for orthopaedic oncologists as the overall survival and activity level of young patients with osteosarcoma continues to improve. This study hypothesised that increased extracortical osseointegration at the bone-implant shoulder will improve stress transfer adjacent to the implant, as evidenced by reduced cortical bone loss, radiolucent line progression and implant failure in young patients (<20-years) following DFR surgery.MethodsTwenty-nine patients of mean age 13.09±0.56y received a primary DFR. The clinical outcome of 11 CPS®, 10 GMRS®, 5 Stanmore® and 3 Repiphysis® implants were evaluated over a mean follow-up period of 4.25±0.55y. The osseous response to an implant shoulder composed of either a hydroxyapatite-coated grooved ingrowth collar (Stanmore®), a porous metal coating (GMRS®) or a polished metal surface (Repiphysis®) was quantified radiographically. ResultsAll (100.0%) of the Stanmore® implants, 90.0% of GMRS®, 81.8% of CPS® and 33.3% of the Repiphysis® implants survived. Significantly increased extracortical bone and osseointegration was measured adjacent to the Stanmore® implants when compared with the GMRS® and Repiphysis® implants (p<0.0001 in both cases). Significantly decreased cortical loss was identified in the Stanmore® group (p=0.005, GMRS® and p<0.0001, Repiphysis®) and at 3-years, the progression of radiolucent lines was reduced when compared with the GMRS® and Repiphysis® implants (p=0.012 and 0.026 respectively). ConclusionsImplants designed to augment osseointegration at the bone-implant shoulder may be critical in reducing short- to mid-term aseptic loosening in this vulnerable DFR patient group. Further longer-term studies are required to confirm these preliminary findings.
Classification Of Body Postures Using Smart Workwear
Authors: Lins Christian , Hein Andreas
Published: March 2022

Background: Despite advancing automation, employees in many industrial and service occupations still have to perform physically intensive work that may have negative effects on the health of the musculoskeletal system. For targeted preventive measures, precise knowledge of the work postures and movements performed is necessary. Methods: Prototype smart work clothes equipped with 15 inertial sensors were used to record reference body postures of 20 subjects. These reference postures were used to create a software-based posture classifier according to the Ovako Working Posture Analysing System (OWAS) by means of an evolutionary training algorithm. Results: A total of 111,275 posture shots were recorded and used for training the classifier. The results show that smart workwear, with the help of evolutionary trained software classifiers, is in principle capable of detecting harmful postures of its wearer. The detection rate of the evolutionary trained classifier (a_ccr = 0.35 for the postures of the back, a_ccr = 0.64 for the arms, and a_ccr = 0.25 for the legs) outperforms that of a TensorFlow trained classifying neural network. Conclusions: In principle, smart workwear – as prototypically shown in this paper – can be a helpful tool for assessing an individual’s risk for work-related musculoskeletal disorders. Numerous potential sources of error have been identified that can affect the detection accuracy of software classifiers required for this purpose.
Acid leaching vermiculite: A multi-functional solid catalyst with strongly electrostatic field and Brönsted acid for depolymerization of cellulose in water
Authors: Chen Xingtao , Tong Dongshen , Fang Zhi , Gao Zhenpeng , Yu Weihua
Published: March 2022

Vermiculite is a natural, environmentally friendly mineral, which is widely used in construction, thermal insulation, metallurgy, and agriculture. In this study, vermiculite and acid-activated vermiculite was used as a solid acid catalyst for the hydrolysis of cellulose in water. The vermiculite based solids were characterized by XRD, FT-IR, and BET. The effects of reaction time, reaction temperature, mass ratio and water amount on the reaction were also investigated in the batch reactor. The results showed that the highest total reducing sugars (TRS) yield of 40.1% could be obtained on the vermiculite activated by 35(wt)% H2SO4 with the mass ratio of catalyst to cellulose of 0.18 and water to cellulose of 16 at 478 K for 3.5 h. The acid-activated vermiculite was also a stable catalyst through calcination at 628 K and the yield of TRS decreased from 40.1% to 36.2% after three times reuse. The results showed that the crystal structure of vermiculite was almost destroyed and the surface -OH groups increased after the acid treatment. However, the synergistic effect of strongly electrostatic polarization and Brönsted acid was responsible for the efficient conversion of cellulose. Finally, the mechanism of cellulose hydrolysis on the acid-activated vermiculite was suggested. This work provides a promising strategy to design an efficient solid catalyst for the cellulose hydrolysis, and also expands the use of vermiculite in a new field.
High CS Expression Leads to Poor Prognosis Associated with Immune Invasion of Lung Adenocarcinoma
Authors: Feng Ran , Hong Shihao , Bian Tingting , Gu Xue , Zheng Miaosen , Liu Jian , Liu Yifei
Published: March 2022

Background
Citrate synthase (CS) is the initial rate-limiting enzyme in tricarboxylic acid cycle. We found that its expression is up-regulated in many cancers. Previous studies on the relationship between the expression of CS and (LUAD) have not been reported till date.
Methods
we systematically analyzed several online databases, such as TCGA and GEO database, TIMER database, Kaplan Meier plotter, Ualcan database and Linkedomics database.
Results
we found that it was highly expressed in patients with LUAD. The high expression of CS caused poor prognosis in LUAD patients, and it was associated with some clinical traits. This indicates that it may be an independent prognostic factor. At the same time, we found that high CS expression was associated with the decline of immune-infiltrating B cells. While exploring the mechanism, we found that highly expressed CS mainly interacts with ATP5B and KPNB1 proteins. Moreover, CS is highly expressed in regulating the cell cycle, P53 binding site and oxygen binding pathways.
Conclusions
Our results confirmed CS was associated with LUAD and affected immune cell infiltration in LUAD. This provided a basis for further use of CS as a new therapeutic target and prognostic indicator of patients with LUAD.
Analytical prediction of cutting forces in cylindrical turning of 304 stainless steel using unequal division shear zone theory
Authors: Li BingLin , Zhang Rui
Published: March 2022

In order to establish a more accurate prediction model of turning forces, this paper proposed an analytical model for cylindrical turning with the consideration of the effect of the main cutting edge angle and the nose radius. Meanwhile, the unequal division shear zone theory in orthogonal free cutting is extended and applied to the oblique non-free cutting in the interaction between the chip units. To take into account the real tool nose geometry, the engaged part in the rounded nose is discretized into a set of cutting edge units. The geometrical parameters associated with the cutting edge units are analysed by using the coordinate transformation approach. Then, the improved oblique cutting model is developed for each cutting edge unit to acquire the component forces along the tool rake face. Finally, the resultant cutting forces in the turning process are calculated by the numerical integration method. To verify the effectiveness of the proposed model, the turning force experiment of 304 stainless steel was carried out by changing the cutting conditions, the main edge cutting angle and the nose radius. Through the comparative analysis between the measured results and the calculation values of the proposed model, it was found that the analytical prediction of cutting force is in good agreement with the experiment.
The first complete mitochondrial genome sequences for Ulidiidae and phylogenetic analysis of Diptera
Authors: xing kun , Kang Chen , Zhao Fei
Published: March 2022

Background: Tetanops sintenisi is a pest that mainly damages the roots of quinoa ( Chenopodium quinoa ) and it is first discovered in China in 2018. Methods and Results: Here, the complete mitochondrial genome (mitogenome) of T. sintenisi was sequenced and compared with the mitogenomes of other Diptera species. The results revealed that the mitogenome of T. sintenisi is 15763 bp in length (GenBank accession number: MT795181) and is comprised of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA genes, and a non-coding A+T-rich region (959 bp). The highly conserved gene arrangement of the mitogenome of T. sintenisi was identical to that of other Diptera insects. Twelve PCGs contained the typical insect start codon ATN, while cox1 had CGA as the start codon. The genes cox2 , nad4 , and nad1 contained an incomplete termination codon T; nad3 , nad5 , and cob contained the complete termination codon TAG; and the remaining seven PCGs contained the termination codon TAA. All tRNA genes were predicted to fold into the typical cloverleaf secondary structure. Phylogenetic analysis of 48 species based on the mitogenome sequence revealed that T. sintenisi clustered with the Tephritidae family, indicating that T. sintenisi and Tephritidae have a close phylogenetic relationship. Conclusions: The phylogenetic relationship of T. sintenisi based on the mitogenome was consistent with the traditional morphological taxonomy, according to which T. sintenisi belongs to the family Otitidae, which is closely related to the family Muscidae.
Drilling Fluid Properties Prediction: A Machine Learning Approach to Automate Laboratory Experiments
Authors: Aljubran Mohammad J. , AlBahrani Hussain I. , Ramasamy Jothibasu , Magana-Mora Arturo
Published: March 2022

Providing access to underground energy resources such as oil, gas, and geothermal energy requires drilling through subterranean rock formations. Drilling fluids are commonly used to enable this drilling process by serving a variety of functions. Chief among these is circulating into the drilled wellbore to remove the produced rock cuttings. Other functions include exerting the hydrostatic pressure necessary to prevent the flow of underground fluids into the wellbore while drilling, minimizing the invasion of solids and undesired fluids into the wellbore rock, and ensuring the fluid remains flowable within the means of the pumps available on site. To assess the ability of a drilling fluid to serve these functions, it has to have certain rheological properties, which are conventionally measured using specialized equipment. Considering that the formulation and components of these drilling fluids can vary greatly for different scenarios, the process of preparing samples and measuring their rheological properties, which is usually performed on a daily basis on a drilling site, is unavoidably time-consuming, repetitive, and error-prone. Based on this, it is apparent that there is a need for a computational model that can accurately predict drilling fluids properties based on the proposed concentration of their components without the need for further laboratory testing. This study describes a novel methodology to train a machine learning model derived from over 6,878 drilling fluid formulations to successfully predict water-based drilling fluids properties with a resulting R2 of 91.07 ± 6.35%.
Low FTO expression serves as a potential biomarker for diagnosis and prognosis of patients with liver cancer
Authors: Jiao Yan , Shi Hong Yun , Fu Chang , Quan Pan Pan , Zhang Li Yue
Published: March 2022

Background: As an important part of m6A modification, FTO plays a carcinogenic effect in cancer. In this study, we aim to explore whether FTO could be a new target for diagnosis and prognostic of patients with liver cancer.Material and Methods: We obtained and analysis data from TCGA database by using R software (version 3.5.1). The box plot was used to analyze the pattern of FTO expression. Receiver operating characteristic (ROC) curve was performed to evaluate diagnostic value of FTO expression in liver cancer. Fisher’s exact and chi-square tests were used to analyze relationship between FTO expression and clinical pathological characteristics. Kaplan-Meier curve and Cox regression analysis were used to explore the prognostic value of FTO in liver cancer.Results: We observed that FTO was down-regulated in liver cancer and was associated with patients’ age. ROC curve showed that FTO expression had good clinical diagnostic value. Our results also revealed that FTO expression was associated with age, histologic grade, M classification, vital status, and overall survival (OS) of patients. Low FTO expression decreased OS, and was an independent predictor of poor prognosis in patients with liver cancer.Conclusion: FTO could serves as a potential biomarker for the diagnosis and prognosis of liver cancer
Relationship between work-family conflict on work engagement in Chinese nurses: multiple mediating effects of anxiety and emotional intelligence
Authors: Chang Hongjuan , Liu Rui , Fang Xiaoxia , Li Xue
Published: March 2022

Background: In recent years, the work service quality of nurses has largely depended on their work engagement, which is extremely important and cannot be underestimated. Therefore, it is utmost importance to investigate the factors that can improve the work engagement of nurses, and the improvement in the level of work engagement of nurses is of great practical significance to the nursing service industry. A balance between work and family can promote the work engagement of nurses. This study aims to develop a structural model to assess the impact of work-family conflict on work engagement among Chinese clinical nurses using multiple mediators to clarify the underlying mechanisms of this relationship.Methods: This is a cross-sectional study involving 1,177 registered nurses from a hospital in China, which provided the work-family conflict, work engagement, anxiety, and emotional intelligence scales. AMOS 23.0 was used for the path analysis of multiple mediation models.Results: The total score of the work engagement of nurses was 58.49 ± 8.52, and the average score was 3.66 ± 0.53. Work-family conflict had a significant positive correlation with anxiety (r = 0.382, P < 0.001) and a significant negative correlation with work engagement (r = -0.381, P < 0.001) and emotional intelligence (r = -0.200, P < 0.001); anxiety had a significant negative correlation with work engagement (r = -0.326, P < 0.001) and emotional intelligence (r = -0.188, P < 0.001); and work engagement had a significant positive correlation with emotional intelligence (r = 0.326, P < 0.001). Emotional intelligence and anxiety partially mediated the relationship between work-family conflict and work engagement, with effect values of -0.092 and -0.075, respectively. The chain-mediating effect of anxiety and emotional intelligence exhibited a value of -0.05. The total indirect effect had a value of -0.172.Conclusions: Thus, it is suggested that hospital managers should take measures to alleviate work-family conflict, relieve anxiety, and improve the work engagement and emotional intelligence of nurses.
Simulated Interprofessional Education Program For Training Clinical Critical Thinking In Medical Nursing Students: A Mixed Method Study
Authors: Zhou Xin-yi , Wang Yan-feng , Dou Chun-xia , Tian Xiao-ying , Su Jin , Chen Yan-ya , Yan Feng-xia , Yang Qiao-hong , Wang Wenru
Published: March 2022

Background: Simulation teaching, which cultivates cooperation and communication in the interprofessional team, has become a suitable type of interprofessional cooperation in college students' health professional courses. This study aimed to explore the intervention effect of simulated interprofessional education (SIPE) on clinical critical thinking of medical nursing students. Methods: This study was a mixed method research including quantitative and qualitative assessment. Results: A total of 30 medical nursing students were randomly divided into a control group (18) and an experimental group (12). The mean scores on interprofessional cooperation and critical thinking of the experimental group were higher than those of the control group (P < 0.05). Quantitative evaluation was conducted using a scale, and qualitative evaluation was conducted through semi-structured interviews. There were three themes emerged: clarifying team positioning, improving team effectiveness, and optimizing the learning experience. Conclusion: This study found that the SIPE program improved medical nursing students' ability to work in interprofessional teams.
Control of the sandflies vector of leishmaniasis: Results of an entomological survey on the sandflies fauna in Morocco (El Hajeb)
Authors: El-Mouhdi Karima , Boussaa Samia , Fekhaoui Mohammed , Mehanned Smahane , Faraj Chafika , Chahlaoui Abdelkader
Published: March 2022

Background
Sandflies are small midges that are the exclusive vectors of cutaneous and visceral leishmaniasis. These diseases are a public health problem in Morocco. Objective: To determine the composition of the phlebotomine fauna, to study the biodiversity and seasonal distribution of phlebotomine in the province of El Hajeb.
Methods
A total of six stations were surveyed, five of which had recorded cases of indigenous leishmaniasis in recent years. Bi-monthly captures from April to December 2019 were carried out using adhesive trapping in different biotopes.
Results
A total of 14590 sand flies were collected of which males represent 80.3%. The activity of the sand fly population started in April and ended at the end of November. The periods of high abundance were in July, September and November. Morphological identification of fasting males and females (14070 specimens) shows the presence of twelve species belonging to the genus Phlebotomus and Sergentomyia: P. longicuspis (79.4%), P. sergenti (7.2%), S. minuta (4.42%), P. perniciosus (2.7%), P. papatasi (2.05%), P. bergeroti (1.1%), S. schwetzi (1%), P. alexandri (0.7%), S. fallax (0.7%), S. dreyfusi (0.5%), P. ariasi (0.2%) and S. antennata (0.1%). The in-depth analysis shows that the leishmaniasis vector species predominate the population of collected sandflies and the risk periods are spread during the summer and autumn seasons.
Conclusion
The present study is the result of an entomological survey at the level of El Hajeb in central Morocco; it gives information on the captured species of sandflies. Therefore, it offers to the decision-makers an important tool to carry out effective vector control actions to limit the risk of bites and to limit the transmission of leishmaniasis.
Implementation performance evaluation of hierarchical diagnosis and treatment system in China based on a sustainable perspective
Authors: Wu Qinde , Xie Xianyu
Published: March 2022

Hierarchical diagnosis and treatment system is essential for addressing the problem of 'difficult and expensive medical treatment'. But little research has focused on a sustainable perspective when evaluating the hierarchical diagnosis and treatment system performance. Using data from a total of 69 sample data in Fujian Province from 2018 to 2020 as representativeness, we explore the position of the hierarchical diagnosis and treatment policies, clarify the important restrictive factors presently, to better reflect the main contradictions in the Hierarchical Diagnosis and Treatment System in China. We find that the average value of the implementation performance of Hierarchical Diagnosis and Treatment System was > 0.5, which was at Level III. "Number of new technologies and new projects performed by hospitals and primary health technicians per 100 (B 3 )", "Number of people per 100 health technicians in the hospitals to the primary medical institutions for guidance (B 2 )", "Contracted coverage rate of family doctors (F 3 )" and "Proportion of master’s and doctor’s degrees of hospital health technicians (A6)" are important factors to promote the performance of Hierarchical Diagnosis and Treatment System at this stage. Our results suggest that policy makers need to smooth the communication and interaction channel of professional disciplines and fully play to the role of medical information technology, pay attention to the construction of family doctors and improve the service level of first diagnosis at the primary level, and improve the medical service capacity of the hospitals through supplement high-quality medical personnel.
Angiectatic nasal polyps with pleomorphism- a diagnostic pitfall
Authors: Zhao Xiaoli , Yue Changli , Xing Li , Li Rui , zhang Yong , Yin Hongyan , Liu Honggang , Piao Yingshi
Published: March 2022

Background Angiectatic nasal polyps with pleomorphism (PANP) are rare benign lesions of nose and paranasal sinuses. Clinical manifestation includes nose obstruction, bleeding and headache. The demonstrating bone-damaged lesion by imaging as well as the enlarged and pleomorphic stromal cells with nuclear atypia by morphology simulate a malignant process, which may be misdiagnosed. The aim of the present study is to generalise the features of PANP in case of potential clinical and pathological pitfall of diagnosis. Methods Thirteen patients diagnosed as PANP were retrospectively analyzed in the Pathology Department of Beijing Tongren Hospital Affiliated to Capital Medical University from August 2014 to December 2019. Immunohistochemical staining with CD34, CK, Vim, Calponin, Ki67,Bcl-2, and STAT-6 was performed with envision-two steps method.Results PANP is a benign tumor presenting with gross variegated tan to gray soft fleshy tissue with foci of obvious hemorrhage and necrosis. The imaging shows internal heterogeneous hyperintensity with a peripheral hypointense rim while postcontrast images display a strong nodular and patchy enhancement. Vimentin (Vim) stain was consistently positive , while negative for CD34, STAT-6 and Bcl-2 (focal positive in two cases). Calponin and CK stain was positive in nine cases, respectively . Conclusions PANP is a clinically rare tumor which may stimulate malignancy lesion. Recognizing of characteristic features in these thirteen patients would be beneficial to avoid misdiagnosis and unnecessary aggressive treatment.
Molecular Response Mechanism of Pseudomonas Nicosulfuronedens LAM1902 to A Typical Sulfonylurea Herbicide Nicosulfuron
Authors: Li Miaomiao , Li Qingqing , Yao Jun , Sunahara Geoffrey , Duran Robert , Zhang Qinghua , Ruan Zhiyong
Published: March 2022

The overuse of the herbicide nicosulfuron (NS) has become a global environmental concern. As a potential bioremediation technology, the microbial degradation of NS shows much promise; however, the detailed mechanisms of microbial taxa responding to NS exposure require further study. An isolated soil-borne bacteria Pseudomonas nicosulfuronedens LAM1902 displaying NS degradabilities was used to characterize the molecular responses to NS exposure, and LAM1902 can degrade exceed 95% of 50 mg/L nicosulfuron under the optimal conditions. Using transcriptomic sequencing, RNA-Seq results indicated that 1102 differentially expressed genes (DEGs) were up-regulated and 702 down-regulated in response to NS. Among them, “ABC transporters,” “sulfur metabolism,” and “ribosome” gene pathways were significantly enriched (p ≤ 0.05). Several gene pathways involved in the glycolysis and pentose phosphate pathways, a two-component regulation system, as well as in bacterial chemotaxis metabolisms were up-regulated under NS exposure. Surprisingly, NS exposure showed positive effects on the production of oxalic acid that is synthesized by genes encoding glycolate oxidase through the glyoxylate cycle pathway. The results suggest that P. nicosulfuronedens LAM1902 adopt acid metabolites production strategies in response to NS, with concomitant NS degradation. Meanwhile, strain LAM1902 likely grows and survives amid NS stress by increased energy production. The present studies provide a glimpse at the molecular response of microorganisms to sulfonylurea pesticide toxicity and a potential framework for future mechanistic studies.
TEDC2 correlated with prognosis and immune microenvironment in lung adenocarcinoma
Authors: Fang Likui , Yu Wenfeng , Zhu Pengfei , Yu Guocan , Ye Bo
Published: March 2022

Tubulin epsilon and delta complex 2 (TEDC2) is a protein coding gene which function is poorly identified yet. This study aimed to identify the role of TEDC2 in prognosis and immune microenvironment of lung adenocarcinoma (LUAD). Through The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, the mRNA expression of TEDC2 was upregulated in LUAD tissues compared to normal tissues. The protein level of TEDC2 was also higher in LUAD in the Human Protein Atlas. The receiver operating characteristic (ROC) curve showed that high TEDC2 level could distinguish LUAD patients from normal subjects. In addition, the impact of TEDC2 expression on prognosis was evaluated by Kaplan-Meier and Cox regression analyses, and the results suggested that high TEDC2 expression was significantly associated with poor prognosis and was the independent prognostic factor in LUAD. GO and KEGG pathway analyses indicated the co-expressed genes of TEDC2 were mainly related to mitotic cell cycle processes. Importantly, high expression of TEDC2 indicated low infiltration of immune cells, especially dendritic cells and B cells. TEDC2 was also positively correlated with immune checkpoints such as PDCD1, LAG3 and CD276. Taken together, this study preliminarily revealed the clinical significance of TEDC2 in LUAD and provided novel insights into the role of TEDC2 in immune microenvironment.
Gliosarcoma with Extensive Extracranial Metastatic Spread and Familial Coincidence: An Illustrative Case
Authors: Friker Lea L. , Tzaridis Theophilos , Enkirch Simon Jonas , Lüders Christine , Hattingen Elke , Kristiansen Glen , Goschzik Tobias , Waha Andreas , Lütter Christiana , Weller Johannes , Herrlinger Ulrich , Pietsch Torsten , Gessi Marco , Baumert Brigitta , Gielen Gerrit H.
Published: March 2022

Gliosarcoma is a rare histopathological subtype of glioblastoma. Metastatic spreading is very unusual. In this report, we illustrate a case of gliosarcoma with extensive extracranial metastases with confirmation of histological and molecular concordance between the primary tumor and a metastatic lesion of the lung. Moreover, the case revealed a family coincidence of glial tumors as the patient’s son was diagnosed with a high-grade glioma shortly after the patient’s death. By molecular analysis (Sanger and next generation panel sequencing), we could confirm that both patients carried mutations in the TP53 gene. Interestingly, the detected mutations were located in different exons. According to the current scientific knowledge, even further genetic findings do not explain the aggressive metastatic spread observed in the reported case nor reveal a family tumor syndrome. In conclusion, the molecular mechanisms leading to a metastatic phenotype of gliosarcoma remain unknown and should be studied further.
Evaluation of the Use of GRADE in Dentistry Systematic Reviews and its Impact on Conclusions: A Protocol for a Methodological Study
Authors: Ibrahim Sara , Azab Maria , Miroshnychenko Anna , Brignardello-Petersen Romina
Published: March 2022

Background: There is a growing body of evidence of systematic reviews (SRs) with varying degrees of methodological quality. The Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach allows SR authors to assess the certainty of the evidence they found and transparently relay their conclusions. As there appears to be infrequent utilization of GRADE in the field of dentistry, to identify the impact of GRADE, the aim of this study is to evaluate the use of GRADE in the dental literature and determine whether SRs that use GRADE differ from those that do not with respect to their conclusions.Methods/Design: We will search Ovid MEDLINE for SRs published from 2016 to the present. We will conduct both screening and data extraction independently and in duplicate and use pre-piloted, standardized forms for data extraction. We will determine the frequency of the use of GRADE and the varying levels of certainty in the current literature and evaluate whether GRADE is being used appropriately. We will also evaluate whether SRs not using GRADE differ from those that use GRADE with regards to methodological quality. We will also determine whether the conclusions of SRs that do not use GRADE would change had GRADE been utilized. Additionally, we will evaluate whether SRs using GRADE are more likely to formulate appropriate conclusions compared to SRs that do not use it.Discussion: This study will investigate the frequency of GRADE assessments in dentistry SRs and the impact of GRADE assessments on the conclusions of a SR. It has important implications for both SR authors and users of this type of literature.
Knowledge and Perception regarding Digital Dentistry among Saudi Dental Students
Authors: Al-Ibrahim Ibrahim K. , Alharbi Salwa M. , Alharbi Bandar B. , Alshammary Freah L. , Madfa Ahmed A.
Published: March 2022

The objective of this study was to investigate the usage of digital technologies by dental students in Saudi Arabia and improve dental education outcomes. At random, electronic surveys were distributed to Saudi dental students. Eight hundred sixteen completed questionnaires were returned, with sixteen incomplete questionnaires not analyzed. The questionnaire requested information on gender, age, and level of study, and participation in the study was completely voluntary. Students were asked to complete the questionnaires based on their observations and experiences. Descriptive statistics such as numbers and percentages were used to analyze the collected data. The Chi-square and Fisher's Exact tests were used to assess the results. Among 778 students, 758 (97.43%) had an idea about digital dentistry; however, only 20 (2.6%) did not know about digital dentistry. Intern and clinical students revealed higher knowledge levels than preclinical students (p < 0.01). The majority of the students, 715 (91.9%), get knowledge from college. Regarding digital dentistry during clinical practice, the majority, 677 (87.0%), of the subjects answered yes; however, 101(13.0%) said no. For digital technology use, the majority, 695 (89.3%) of the issues, answered that digital dentistry is helpful in diagnosis; however, only 79 (10.2%) thought that digital dentistry could achieve clinical treatment. The majority of the 766 individuals (98.5%) believed that digital dentistry improved the quality of dental procedures. The majority of students, 737 (94.7%), said that digital dentistry would eventually replace traditional dental services. Regarding higher learning outcomes and the need for skill and training, most sample 765 (98.3%) replied yes to both questions. It can be concluded that students have good knowledge about digital technology, which may have motivated them to practice.
Impact of CeO2 doped ZnO binary metal oxide nanocomposites as clad modified fiber optic VOC gas sensor for exhaled breath analysis
Authors: Balusamy Renganathan , Rao Subha Krishna , A.R Ganesan , Annamalai Deepak Arun
Published: March 2022

The detection of volatile organic compounds (VOCs) in ambient environments has opened up a new field for the quick, risk-free, and potentially low-cost identification of respiratory illnesses. In this context, CeO2 doped ZnO nanocomposites were produced through the sol-gel technique at various ratios (CeO2:ZnO at 1:4, 4:4, and 4:1, respectively), and their gas sensing capability was evaluated and shown using a clad modified fiber optic sensor to study its potential as a VOC detector. The study was based on the concepts of evanescent wave absorption. The modified cladded optical fiber was exposed to the ammonia, ethanol, and methanol gas medium in the testing laboratory. At room/chamber temperatures, the sensor's sensitivity is tested, and was observed that CeO2 doped ZnO at 4:1 ratio exhibited better sensitivity towards ammonia gas vapour.
Structural insights into conformational stability of ESR1 and structure base screening of new potent inhibitor for the treatment of Breast Cancer
Authors: Chopra Ishita , Panwar Umesh , Madhavi Maddala , Bhrdwaj Anushka , Soni Lovely , Sharma Khushboo , Parihar Abhyuday Singh , Mohan Vineeth Pazharathu , Prajapati Leena , Joshi Isha , Sharma Rashmi , Agrawal Shweta , Alzahrani Khalid J , Hussain Tajamul , Nayarisseri Anuraj , Singh Sanjeev Kumar
Published: March 2022

Estrogen receptor alpha (ERα), a nuclear receptor proteinencoded by the estrogen receptor1 (ESR1) gene,is an important biomarker in breast cancer diagnosis. Any dysregulation in its expression can actively implicate the development and progression of the disease. ERα is abnormally expressed in around 60% of the active cases, making it animportant therapeutic target. In this study, we report the application of computational approaches to identify suitable drug-like molecules, which share similar ligand binding dynamics with ERα. Structure-based virtual screening(SBVS), docking, and inhibitor dynamics are used to study the ligand binding and interaction profiling of the anticipatedligand molecule, at the active site of the 4-hydroxytamoxifen (OHT) protein (PDB Id: 3ERT). SBVS analysis follows HTVS, SP, and XP protocol in comparison to the ZINC and NCI, to retrieve 20 bestligandhits as effective inhibitors; All the compounds have shown significant interaction with active site residues (Leu346, Thr347, Asp351, Glu353, Trp383, Leu387, and Arg394) of the 4-hydroxytamoxifen. Moreover, the docking study was used to screen the top 5 compounds: ZINC13377936, NCI35753, ZINC35465238, ZINC14726791, and NCI663569. We also, employed molecular dynamics simulations to explore the binding dynamics present at the atomic level. Our MDS results have revealed the compounds (ZINC13377936 and NCI35753) with outstanding binding stability and lesser fluctuations. Both above hits possess a high potential as future therapeutic agents, acting by the mechanism of competitive inhibitionagainst the ERα protein in breast cancer.
Research of the Active Components and Potential Mechanisms of Qingfei Gujin Decoction in the Treatment of Osteosarcoma Based on Network Pharmacology and Molecular Docking
Authors: Yan Qingying , Yang Jiewen , Yao Yongwei , Jia Zhen , Wang Yiqing , Miao Chen , Yan Xiaobo , Xu Yefeng
Published: March 2022

Aim: Qingfei Gujin decoction (QGD) has been shown to be effective against osteosarcoma. This research was aimed to investigate the main active ingredients and potential mechanisms of QGD acting on osteosarcoma through network pharmacology and molecular docking techniques. Methods: The active ingredients and targets of QGD were screened from the TCMSP database, and the predicted targets were obtained from the PharmMapper database. Meanwhile, the targets of osteosarcoma were collected using OMIM, PharmGkb and DisGeNET databases. Then, GO and KEGG enrichment analysis was performed by RStudio. PPI and drug-ingredients-targets network were constructed using Cytoscape 3.2.1 to screen the major active ingredients, key networks and targets. Finally, molecular docking of key genes and their regulatory active ingredients was performed using AutoDockTools-1.5.6 software. Results: 38 active ingredients were collected, generating 89 cross-targets; Quercetin, luteolin, β-sitosterol, and kaempferol were the main active ingredients of QGD acting on osteosarcoma, and major signaling pathways such as PI3K-Akt signaling pathway, MAPK signaling pathway, and IL-17 signaling pathway were observed. TP53, SRC, and ESR1 were identified as key proteins that docked well with their regulated compounds. Conclusion: QGD is effective against osteosarcoma through multi-component, multi-target and multi-pathway. This study was helpful for finding effective targets and compounds for osteosarcoma treatment.
Multimodal Detection of Hateful Messages Using Visual-Linguistic Pre-Trained Deep Learning Models
Authors: Chen Yuyang , Pan Feng
Published: March 2022

Online hateful messages, more commonly known as “hate speech”, have recently become a major social issue. Many studies have shown them to be detrimental for both the individuals and the society. Many online platforms have employed legions of moderators to manually identify and remove these messages, yet such practices are time-consuming, expensive, and often causing mental illness among the reviewers. As a solution, computational methods are applied to automatically identify and remove hateful messages. However, as online discussions are now often dominated by memes, a format that leverages both text and image to express users’ intents, many textual moderation methods have become obsolete. In order to effectively detect a hateful meme, the algorithm must possess strong vision and language fusion capa- bility. In this work, we move closer to this goal by compositely using a Visual-Language Pre-Trained Model, an object detection model and a random forest classifier to achieve a 0.77 AUROC score on the hateful meme detection task, an improvement of 0.15 compared to the best baseline method.
SiO2 and ZnO Hybrid Nanofillers Modified Natural Rubber Latex: Excellent Tensile Properties and Antibacterial Properties
Authors: Liu Ye , Mou Wenjie , Li Jinglin , Fu Xiaomei , Huang Chaojie , Chen Lishui
Published: March 2022

The modification of polymer with nanoparticles has been proved to have good results, but the key of this method is that nanoparticles can maintain uniform dispersion in the polymer. Herein, silica (SiO2 ) and zinc oxide (ZnO) hybrid nanofillers were prepared by self-made dispersion equipment to modify natural rubber latex (NRL). The results show that the uniformly dispersed nano-SiO2 fillers increase the number of entanglements in the molecular chain of NRL, thereby improving the tensile properties. The tensile strength and elongation at break of LZ1S4.2 were increased to 32.61 MPa and 957%, respectively. The increase of the number of entanglements also makes the structure of NRL more compact, and improves the barrier performance and aging resistance. The uniformly dispersed nano-ZnO fillers endow the NRL with excellent antibacterial properties, and the antibacterial rate (For Escherichia coli and Staphylococcus aureus ) is greater than 99.9%. In addition, NRL condoms and gloves incorporating hybrid nanofillers have been proved to have good biocompatibility, and this NRL nanocomposite is expected to be applied to other NRL products.
Giant and Rapidly Growing Malignant Peripheral Nerve Sheath Tumor of the Head and Neck with Pulmonary Metastasis: Case Report and Review of the Literature
Authors: Cao Yuandong , Tao Wei , Jia Ting , Liu Liang , Jiang Chunyu
Published: March 2022

Background：Malignant peripheral nerve sheath tumors (MPNSTs) are a subtype of malignant soft tissue sarcoma arising from peripheral nerve branches or sheaths of peripheral nerve fibers. Most cases are associated with bigger nerve stems, and are more commonly found in the proximal parts of the limbs and trunk, but rarely in the head and neck regions. However, reports on MPNSTs with pulmonary metastasis at the time of initial diagnosis are limited. Here, we present a case of MPNST that is located in the head and neck region with pulmonary metastasis and a literature review. Case description：Here, we report a case of primary malignant peripheral nerve sheath tumor of the head and neck with pulmonary metastasis. The tumor was surgically removed and fused with autogenous bone and artificial bone. The histopathological diagnosis was MPNST. However, the tumor rapidly recurred one month after surgery, and the patient exhibited progressive decline in muscle strength with numbness and an inability to move independently, so the tumor was again surgically removed. Then, she underwent adjuvant radiotherapy, chemotherapy, and antiangiogenic therapy. After three months, the patient’s tumor rapidly progressed into intracranial, neck, and lung lesions, and genetic tests were performed.Conclusions: Giant MPNSTs of the head and neck with pulmonary metastasis are highly aggressive lesions that should primarily be treated in a surgical fashion. Adjuvant radiotherapy should be initiated as soon as the patient recovers from the postoperative wound, preferably within two weeks. Antiangiogenic drugs and radiotherapy may have synergistic effects.
Probing the Nature of Code Mixing and Code Switching From Amharic to Awigni and Its Influence on the Indigenous Language-Awigni
Authors: Techane Adugna
Published: March 2022

The main purpose of this study was to explore the nature of code mixing and code switching from Amharic to Awigni and its influence on the indigenous language-Awigni at Injibara town, Awi zone, Ethiopia. The subjects of the study were from the woredas’ educational bureau, cultural and tourism bureau and public schools located at the town. So as to achieve this goal, a mixed research design was employed. Thirty two respondents were selected from the woredas’ educational bureau, cultural and tourism bureau and public schools through using comprehensive sampling technique were as the participants of the study. Questionnaire, interview, observation, and FGD were utilized to gather data. The findings indicated that different aspects of Awi language is affected due to the influence of Amharic language. Basically, its morphology has been affected. The results also revealed that in the case between Amharic and awigni, there are four basic causes to mix and switch. These include boarder proximity, religious activities, and historical relationship and trade activities. Moreover, speakers mix and switch codes from Amharic due to different relations with Amharic speakers. Finally, it is recommended that collecting the original Awigni language words and sounds from elders should be the task to everyone to save the language.
Polyphenols from Green tea revert CCl4-Induced liver oxidative stress and inflammation associated with high fat diet by activation of NRF-2 and TIMP-1 pathways to neutralize ROS
Authors: Kanwal Hafiza Arooj , majeed wafa , Awan Ambreen Mehmood , Aslam Bilal
Published: March 2022

Hepatic disorders are progressed by oxidative stress, chronic inflammation, hepatocyte death and increased transforming growth factor. Herein we investigated the phytochemical screening of green tea (Camellia sinensis) and analyzed its anti-oxidant and anti-inflammatory potential on liver biomarkers that elaborate therapeutic efficacy of polyphenols (catechins) in rat model associated with CCl4 induced liver injury along high fat diet. Pro-inflammatory cytokines are stimulated by either acute or chronic liver injury and critically triggered the production of inflammation, steatosis, fibrosis and liver carcinoma. We designed HPLC work to isolate the green tea phytochemical constituents quantitatively in a remarkable therapeutic range. Anti-inflammatory and antioxidant activities were carried out in vivo using treatment with green tea polyphenol extract (GTPE 600 mg/kg) that significantly reduced (P < 0,001) pro-inflammatory cytokines like tumor necrosis factor α (TNF α) and interleukin 6 (IL-6) in CCl4-induced hepatic injury in Wistar albino rats. Hepatoprotective potential of GTPE was evaluated through biochemical parameters (complete blood count, liver functional tests and lipid profile), oxidative stress biomarkers (MDA, CAT, GPx and SOD) as well as histopathological analysis. Study of NRF-2 and TIMP-1 pathways through qRT-PCR provided strategy to delay the progression of liver injury into liver fibrosis by neutralizing reactive oxygen species and reverting inflammatory cytokines with the help of GTPE in therapeutic dose. Pharmacological confirmation of current study indicated that green tea possessed incredible anti-oxidative and anti-inflammatory properties, suggesting that polyphenols providing active components (catechins) of crude extract.
Ultrasoic endoscopy guiding to cut scar of esophageal stricture after variceal sclerotherapy
Authors: Zhu Yuandong , Zhang Fulong , Xu Jing , Shi Yan , Wu Bo , Wang Hai , Huang Chaojun
Published: March 2022

Objective: We want to investigate the efficacy of ultrasoic endoscopy guiding to cut scar of esophageal stricture after variceal sclerotherapy.Method: The data of 10 patients with esophageal stricture after esophageal varices sclerotherapy injection(ESI) in our hospital from September 1, 2021 to December 31, 2021 treated by cutting the scar guided by ultrasoic endoscopy were retrospective, and the efficacy was evaluated.Result: The dysphagia was obviously relieved in 9 patients during follow-up, and 1 patient suffered dysphagia again after the treatment.There was no complications of perforation, bleeding and infection among the paitents.Conclusion: Ultrasoic endoscopy guiding to cut scar was safe and reliable, which could reduce the occurrence of perforation and bleeding.