bluuebunny/crossref_metadata_2025 huggingface dataset:
Copper Nanoparticles and Reduced Graphene Oxide as an Electrode Modifier for the Development of an Electrochemical Sensing Platform for Chloroquine Phosphate Determination
Authors: Barreto Francisco Contini , da Silva Martin Kássio Leme , Cesarino Ivana
Published: April 2023

This study describes the use of copper nanoparticles (CuNPs) and reduced graphene oxide (rGO) as an electrode modifier for the determination of chloroquine phosphate (CQP). The synthetized rGO-CuNPs composite was morphologically characterized using scanning electron microscopy and electrochemically characterized using cyclic voltammetry. The parameters were optimized and the developed electrochemical sensor was applied in the determination of CQP using square-wave voltammetry (SWV). The analytical range for the determination of CQP was 0.5 to 110 μmol L−1 (one of the highest linear ranges for CQP considering electrochemical sensors), with limits of detection and quantification of 0.23 and 0.78 μmol L−1, respectively. Finally, the glassy carbon (GC) electrode modified with rGO-CuNPs was used for quantification of CQP in tap water; a study was carried out with interferents using SWV and obtained great results. The use of rGO-CuNP material as an electrode modifier was thus shown to be a good alternative for the development of low-cost devices for CQP analysis.
Reducing Moisture Absorption in Polypropylene Nanocomposites for Automotive Headlamps Using Hydrophobicity-Modified Graphene/Montmorillonite
Authors: Lee Pyoung-Chan , Kang Dongwoo , Oh Ji Taek , Seo Jae Young , Shin Donghyeok , Jung Jae-Uk , Ko Youn Ki , Ha Jin Uk , Kim Myeong-Gi
Published: April 2023

Polypropylene (PP) is used as a housing material in automotive headlamps but can cause fogging as a result of absorbed moisture and temperature differences between the exterior and interior of the housing. In this study, PP was combined with a graphene/montmorillonite hybrid (MMT-G) to yield a nanocomposite with reduced moisture absorption. Crucially, the modified nanofiller had low hydrophilicity and good compatibility with the PP matrix. Notably, the water contact angle of the MMT-G improved by 676%. Furthermore, the maximum moisture absorption of the PP/MMT-G nanocomposites was reduced by up to 11.22% compared to that of commercial PP composites, and the weight of the headlamp housing was decreased by 3.6%. Therefore, the designed nanocomposites are expected to help mitigate headlamp fogging while slightly reducing the housing weight.
Nanomaterials as Promising Additives for High-Performance 3D-Printed Concrete: A Critical Review
Authors: Razzaghian Ghadikolaee Mehrdad , Cerro-Prada Elena , Pan Zhu , Habibnejad Korayem Asghar
Published: April 2023

Three-dimensional (3D) printed concrete (3DPC), as one of the subset of digital fabrication, has provided a revolution in the construction industry. Accordingly, scientists, experts, and researchers in both academic and industry communities are trying to improve the performance of 3DPC. The mix design of all kinds of concrete has always been the most crucial property to reach the best efficiency. Recently, many studies have been performed to incorporate nano- and micro-scale additives to ameliorate the properties of 3DPC. The current study aims to present the main design properties of 3DPC and completely cover both fresh and hardened state characteristics of 3DPC containing different nano- and micro-additives. Our observations illustrate that nanomaterials can be mainly utilized as a thickener to ameliorate the thixotropic behavior and the structural build-up of 3DPC, resulting in higher yield stress and better viscosity recovery. Furthermore, each nanomaterial, through its unique impact, can provide lower porosity and permeability as well as better mechanical strengths for 3DPC. Although much research investigate the fresh properties of 3DPC containing nano and micro additives, future studies are needed to provide better insight into the impact of these kinds of additives on the hardened characteristics of 3DPC. In addition, researchers may devote more research to address the effects of the additives discussed herein on the performance of other kinds of 3DPC such as lightweight, self-compacting, etc. It should be noted that the effect mechanism of nanomaterials on the inter-layer bond strength of 3DPC is another crucial issue that should be investigated in future studies. Furthermore, nano-scale fillers from source of waste and biomass can be attractive additives for future research to achieve high performance of sustainable 3D-printed concrete.
Temperature-Dependent and Time-Resolved Luminescence Characterization of γ-Ga2O3 Nanoparticles
Authors: García-Carrión Marina , Ramírez-Castellanos Julio , Nogales Emilio , Méndez Bianchi
Published: April 2023

The temperature-dependent luminescence properties of γ-Ga2O3 nanoparticles prepared by a precipitation method are investigated under steady-state and pulsed-light excitation. The main photoluminescence (PL) emission at room temperature consists of a single blue band centered around 2.76 eV, which hardly undergoes a blueshift of 0.03 eV when temperature goes down to 4 K. The emission behaves with a positive thermal quenching following an Arrhenius-type curve. The data fitting yields two non-radiative levels affecting the emission band with activation energies of 7 meV and 40 meV. On the other hand, time-resolved PL measurements have also been taken and studied as a function of the temperature. The data analysis has resulted in two lifetimes: one of 3.4 ns and the other of 32 ns at room temperature, which undergo an increase up to 4.5 ns and 65 ns at T = 4 K, respectively. Based on both stationary and dynamic PL results, a model of radiative and non-radiative levels associated with the main emission bands of γ-Ga2O3 is suggested. Finally, by using PL excitation measurements, an estimation of the bandgap and its variation with temperature between 4 K and room temperature were obtained and assessed against O’Donnell–Chen’s law. With this variation it has been possible to calculate the average of the phonon energy, resulting in ⟨ħω⟩ = 10 ± 1 meV.
Isolation and Identification of Multidrug-Resistant Klebsiella pneumoniae Clones from the Hospital Environment
Authors: Córdova-Espinoza María Guadalupe , Giono-Cerezo Silvia , Sierra-Atanacio Erika Gabriela , Escamilla-Gutiérrez Alejandro , Carrillo-Tapia Eduardo , Carrillo-Vázquez Laura Isabel , Mendoza-Pérez Felipe , Leyte-Lugo Martha , González-Vázquez Raquel , Mayorga-Reyes Lino , González-Vázquez Rosa
Published: April 2023

Global dispersion, hospital outbreaks, and lineage relationships between emerging antibiotic-resistant strains such as Klebsiella pneumoniae are of public health interest. This study aimed to isolate and identify K. pneumoniae clones from third-level healthcare hospitals in Mexico to establish their multidrug-resistant phenotype, phylogeny, and prevalence. Biological and abiotic surface samples were used to isolate K. pneumoniae strains and to test their antibiotic susceptibility to classify them. The housekeeping genes: gapA, InfB, mdh, pgi, phoE, ropB, and tonB were used for multilocus sequence typing (MLST). Phylogenetic networks were constructed with 48 strains. Isolated strains (93) were mainly from urine and blood, 96% were resistant to ampicillin as expected, 60% were extended-spectrum β-lactamases (ESBL), 98% were susceptible to ertapenem and meropenem and 99% were susceptible to imipenem, 46% were multi-drug resistant (MDR), 17% were extensively-drug resistant (XDR), 1% were pan-drug resistant (PDR), and 36% were not classified. The tonB, mdh, and phoE genes were the most variable, and the InfB gene showed positive selection. The most prevalent sequence types (STs) were ST551 (six clones), ST405 (six clones), ST1088 (four clones), ST25 (four clones), ST392 (three clones), and ST36 (two clones). ST706 was PDR, and ST1088 clones were MDR; neither of these STs has been reported in Mexico. The strains analyzed were from different hospitals and locations; thus, it is important to maintain antibiotic surveillance and avoid clone dissemination to prevent outbreaks, adaptation to antibiotics, and the transmission of antibiotic resistance.
Immune Response of the Host and Vaccine Development
Authors: Długosz Ewa , Wesołowska Agnieszka
Published: April 2023

Vaccines are one of the greatest achievements of modern medicine, offering an effective way to fight and control infectious diseases [...]
Challenges in the Vaccination of the Elderly and Strategies for Improvement
Authors: Soegiarto Gatot , Purnomosari Dewajani
Published: April 2023

In recent years, the elderly has become a rapidly growing proportion of the world’s population as life expectancy is extending. Immunosenescence and inflammaging contribute to the increased risk of chronic non-communicable and acute infectious diseases. Frailty is highly prevalent in the elderly and is associated with an impaired immune response, a higher propensity to infection, and a lower response to vaccines. Additionally, the presence of uncontrolled comorbid diseases in the elderly also contributes to sarcopenia and frailty. Vaccine-preventable diseases that threaten the elderly include influenza, pneumococcal infection, herpes zoster, and COVID-19, which contribute to significant disability-adjusted life years lost. Previous studies had shown that conventional vaccines only yielded suboptimal protection that wanes rapidly in a shorter time. This article reviews published papers on several vaccination strategies that were developed for the elderly to solve these problems: more immunogenic vaccine formulations using larger doses of antigen, stronger vaccine adjuvants, recombinant subunit or protein conjugated vaccines, newly developed mRNA vaccines, giving booster shots, and exploring alternative routes of administration. Included also are several publications on senolytic medications under investigation to boost the immune system and vaccine response in the elderly. With all those in regard, the currently recommended vaccines for the elderly are presented.
In Situ Synthesis of a Tumor-Microenvironment-Responsive Chemotherapy Drug
Authors: Wang Xiupeng , Oyane Ayako , Inose Tomoya , Nakamura Maki
Published: April 2023

Current chemotherapy still suffers from unsatisfactory therapeutic efficacy, multi-drug resistance, and severe adverse effects, thus necessitating the development of techniques to confine chemotherapy drugs in the tumor microenvironment. Herein, we fabricated nanospheres of mesoporous silica (MS) doped with Cu (MS-Cu) and polyethylene glycol (PEG)-coated MS-Cu (PEG-MS-Cu) as exogenous copper supply systems to tumors. The synthesized MS-Cu nanospheres showed diameters of 30–150 nm with Cu/Si molar ratios of 0.041–0.069. Only disulfiram (DSF) and only MS-Cu nanospheres showed little cytotoxicity in vitro, whereas the combination of DSF and MS-Cu nanospheres showed significant cytotoxicity against MOC1 and MOC2 cells at concentrations of 0.2–1 μg/mL. Oral DSF administration in combination with MS-Cu nanospheres intratumoral or PEG-MS-Cu nanospheres intravenous administration showed significant antitumor efficacy against MOC2 cells in vivo. In contrast to traditional drug delivery systems, we herein propose a system for the in situ synthesis of chemotherapy drugs by converting nontoxic substances into antitumor chemotherapy drugs in a specific tumor microenvironment.
Nanostructured Lipid Carrier Co-Loaded with Docetaxel and Magnetic Nanoparticles: Physicochemical Characterization and In Vitro Evaluation
Authors: Idris Auni Hamimi , Che Abdullah Che Azurahanim , Yusof Nor Azah , Asmawi Azren Aida , Abdul Rahman Mohd Basyaruddin
Published: April 2023

Lung cancer is currently the most prevalent cause of cancer mortality due to late diagnosis and lack of curative therapies. Docetaxel (Dtx) is clinically proven as effective, but poor aqueous solubility and non-selective cytotoxicity limit its therapeutic efficacy. In this work, a nanostructured lipid carrier (NLC) loaded with iron oxide nanoparticles (IONP) and Dtx (Dtx-MNLC) was developed as a potential theranostic agent for lung cancer treatment. The amount of IONP and Dtx loaded into the Dtx-MNLC was quantified using Inductively Coupled Plasma Optical Emission Spectroscopy and high-performance liquid chromatography. Dtx-MNLC was then subjected to an assessment of physicochemical characteristics, in vitro drug release, and cytotoxicity. Dtx loading percentage was determined at 3.98% w/w, and 0.36 mg/mL IONP was loaded into the Dtx-MNLC. The formulation showed a biphasic drug release in a simulated cancer cell microenvironment, where 40% of Dtx was released for the first 6 h, and 80% cumulative release was achieved after 48 h. Dtx-MNLC exhibited higher cytotoxicity to A549 cells than MRC5 in a dose-dependent manner. Furthermore, the toxicity of Dtx-MNLC to MRC5 was lower than the commercial formulation. In conclusion, Dtx-MNLC shows the efficacy to inhibit lung cancer cell growth, yet it reduced toxicity on healthy lung cells and is potentially capable as a theranostic agent for lung cancer treatment.
Dispersion Caused by the Penetration Effect in X-ray Compressors
Authors: Yang Chuan , Hu Kai , Zhu Ye , Wang Xiaofan , Li Qinming , Xu Zhongmin , Wu Juhao , Zhang Weiqing
Published: April 2023

Chirped X-ray pulse compression is a promising approach for generating ultra-short X-ray free electron laser (XFEL) pulses. The design of X-ray pulse compressors requires the careful control of group delay dispersion (GDD), which plays a critical role in achieving optimal compression. However, the penetration dispersion of crystals and multilayers can induce an extra GDD, which may result in over-compression or under-compression. In this study, we investigate the penetration dispersion of crystals and multilayers theoretically and numerically. Our results indicate that the extra GDD induced by the penetration effect increases as the bandwidth of the rocking curve decreases. Moreover, the extra GDD is nonlinear and can be mitigated by optimizing the configuration of X-ray pulse compressors. This work provides insights into the dispersion compensation and configuration optimization of X-ray pulse compressors, which are essential for generating ultra-short XFEL pulses.
The Optical Inverse Problem in Quantitative Photoacoustic Tomography: A Review
Authors: Wang Zeqi , Tao Wei , Zhao Hui
Published: April 2023

Photoacoustic tomography is a fast-growing biomedical imaging modality that combines rich optical contrast with a high acoustic resolution, at depths in tissues. Building upon the foundation of this technique, novel quantitative photoacoustic tomography fully leverages its advantages while further delivering improved quantification capabilities to produce high-accuracy concentration estimates, which has attracted substantial research interest in recent years. The kernel challenge associated with quantitative photoacoustic tomography is an optical inverse problem aiming to recover the absorption coefficient distribution from the conventional photoacoustic image. Although the crucial importance of the optical inversion has been widely acknowledged, achieving it has remained a persistent challenge due to the inherent non-linearity and non-uniqueness. In the past decade, numerous methods were proposed and have made noticeable progress in addressing this concern. Nevertheless, a review has been conspicuously absent for a long time. Aiming to bridge this gap, the present study comprehensively investigates the recent research in this field, and methods identified with significant value are introduced in this paper. Moreover, all included methods are systematically classified based on their underlying principles. Finally, we summarize each category and highlight its remaining challenges and potential future research directions.
GaAs Linear Polarizer with a High Extinction Ratio for Extended Short-Wave Infrared Detection
Authors: Shi Leidong , Lu Lidan , Chen Guang , Feng Yulin , He Yanlin , Ren Guanghui , Ou Jianzhen , Zhu Lianqing
Published: April 2023

Metasurfaces have shown an unprecedented ability to modulate electromagnetic waves at subwavelength scales, especially polarized optical metasurfaces, applied for imaging, navigation and detection. In this work, a kind of efficient all-dielectric diatomic metasurface for polarization and phase changing, consisting of a pair of GaAs nanopillar and nanocube, is proposed. By adjusting the unit cell structural parameters, the polarization state can be controlled and adjusted at the short-wave infrared (SWIR) band (1~3 μm). At the wavelength of 2125 nm, the maximum transmission efficiency, the extinction ratio and the linear polarization degree can reach 93.76%, 40.99 dB and 0.99, respectively. Overall, this all-dielectric diatomic metasurface has broad application potential in extended SWIR polarization detection.
Infrared Gas Detection and Concentration Inversion Based on Dual-Temperature Background Points
Authors: Wu Sipeng , Zhong Xing , Qu Zheng , Wang Yuanhang , Li Lei , Zeng Chaoli
Published: April 2023

Gas detection based on infrared thermal imaging is applied in many areas, but it is generally applied as a qualitative detection method to observe the target area; on the other hand, quantitative research on gas concentration is less common, the measurement accuracy is poor, and the calculation method of concentration in the commonly adopted transmission model is also complicated. In this paper, based on the radiance transfer model of gas infrared imaging technology, the influence of gas concentration, gas temperature, and background temperature on gas imaging detection is investigated, a gas detection and concentration inversion method based on dual-temperature background points is proposed, and the effects of the choice of reference band on background temperature correction are analyzed in relation to the changing trend of dual-band radiance difference. To verify the effectiveness of this method, a gas detection system with dual-temperature background spots was constructed in this paper utilizing a cooled mid-wave infrared focal plane detector plus a reference filter and a measurement filter, which achieved a promising concentration accuracy of less than 10% for carbon dioxide at a detectable range. Meanwhile, an infrared imaging system with a noise equivalent temperature difference (NETD) of 40 mK was employed to simulate the detection of methane, which enables the detection and concentration inversion of methane gas at a minimum concentration of 500 ppm·m at a distance of 1 km, which proves the capability of long-range detection.
Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Six Ipomoea Species and the Identification of Anthocyanin-Related Members in Sweet Potatoes
Authors: Li Maoxing , Zhou Yuanping , Li Kaifeng , Guo Huachun
Published: April 2023

Sweet potatoes (Ipomoea batatas) are one of the important tuberous root crops cultivated worldwide, and thier storage roots are rich in antioxidants, such as anthocyanins. R2R3-MYB is a large gene family involved in various biological processes, including anthocyanin biosynthesis. However, few reports about the R2R3-MYB gene family of sweet potatoes have been released to date. In the present study, a total of 695 typical R2R3-MYB genes were identified in six Ipomoea species, including 131 R2R3-MYB genes in sweet potatoes. A maximum likelihood phylogenetic analysis divided these genes into 36 clades, referring to the classification of 126 R2R3-MYB proteins of Arabidopsis. Clade C25(S12) has no members in six Ipomoea species, whereas four clades (i.e., clade C21, C26, C30, and C36), including 102 members, had no members in Arabidopsis, and they were identified as Ipomoea-specific clades. The identified R2R3-MYB genes were unevenly distributed on all chromosomes in six Ipomoea species genomes, and the collinearity analysis among hexaploid I. batatas and another five diploid Ipomoea species suggested that the sweet potato genome might have undergone a larger chromosome rearrangement during the evolution process. Further analyses of gene duplication events showed that whole-genome duplication, transposed duplication, and dispersed duplication events were the primary forces driving the R2R3-MYB gene family expansion of Ipomoea plants, and these duplicated genes experienced strong purifying selection because of their Ka/Ks ratio, which is less than 1. Additionally, the genomic sequence length of 131 IbR2R3-MYBs varied from 923 bp to ~12.9 kb with a mean of ~2.6 kb, and most of them had more than three exons. The Motif 1, 2, 3, and 4 formed typical R2 and R3 domains and were identified in all IbR2R3-MYB proteins. Finally, based on multiple RNA-seq datasets, two IbR2R3-MYB genes (IbMYB1/g17138.t1 and IbMYB113/g17108.t1) were relatively highly expressed in pigmented leaves and tuberous root flesh and skin, respectively; thus, they were identified to regulate tissue-specific anthocyanin accumulation in sweet potato. This study provides a basis for the evolution and function of the R2R3-MYB gene family in sweet potatoes and five other Ipomoea species.
Divergent Metabolic Changes in Rhizomes of Lowland and Upland Switchgrass (Panicum virgatum) from Early Season through Dormancy Onset
Authors: Palmer Nathan A. , Sarath Gautam , Bowman Michael J. , Saathoff Aaron J. , Edmé Serge J. , Mitchell Robert B. , Tobias Christian M. , Madhavan Soundararajan , Scully Erin D. , Sattler Scott E.
Published: April 2023

High-biomass-yielding southerly adapted switchgrasses (Panicum virgatum L.) frequently suffer from unpredictable winter hardiness at more northerly sites arising from damage to rhizomes that prevent effective spring regrowth. Previously, changes occurring over the growing season in rhizomes sampled from a cold-adapted tetraploid upland cultivar, Summer, demonstrated a role for abscisic acid (ABA), starch accumulation, and transcriptional reprogramming as drivers of dormancy onset and potential keys to rhizome health during winter dormancy. Here, rhizome metabolism of a high-yielding southerly adapted tetraploid switchgrass cultivar, Kanlow—which is a significant source of genetics for yield improvement—was studied over a growing season at a northern site. Metabolite levels and transcript abundances were combined to develop physiological profiles accompanying greening through the onset of dormancy in Kanlow rhizomes. Next, comparisons of the data to rhizome metabolism occurring in the adapted upland cultivar Summer were performed. These data revealed both similarities as well as numerous differences in rhizome metabolism that were indicative of physiological adaptations unique to each cultivar. Similarities included elevated ABA levels and accumulation of starch in rhizomes during dormancy onset. Notable differences were observed in the accumulation of specific metabolites, the expression of genes encoding transcription factors, and several enzymes linked to primary metabolism.
Effects of Genotype and Climate on Productive Performance of High Oleic Carthamus tinctorius L. under Rainfed Conditions in a Semi-Arid Environment of Sicily (Italy)
Authors: Licata Mario , Farruggia Davide , Iacuzzi Nicolò , Matteo Roberto , Tuttolomondo Teresa , Di Miceli Giuseppe
Published: April 2023

Safflower (Carthamus tinctorius L.) is a promising oilseed crop for cultivation in central Southern Italy due to its high tolerance to drought and salinity stress and appreciable seed and oil yields. The genetic diversity of cultivars and climate factors can affect fatty acid composition and yield traits. This study aimed to assess the effects of genotype and climate conditions on the productive performance of eight high oleic safflower genotypes under rainfed conditions in Sicily (Italy). These genotypes were compared to the Montola 2000 cultivar, which was used as a reference. Tests were carried out during the growing seasons of 2014–2015 and 2015–2016. The experimental design was a randomized complete block design with three replications. Morphological and yield components were significantly affected by genotype while the year had a significant effect on plant height only. In general, CTI 17 produced the highest seed yield (1.40 t ha−1) and oil yield (0.58 t ha−1). The seed oil content was found on 40.2% of dry matter, on average. The “genotype” factor significantly affected oil content and fatty acid composition. Oleic acid content was on average 66.1% and did not vary greatly over the two growing seasons. The above- and belowground plant parts showed the highest carbon content and the lowest nitrogen content as a percentage of dry matter. The results indicate that, under rainfed conditions, yield parameters of high oleic safflower genotypes can be profitable in southern Italy though significantly dependent upon genotype.
Effects of Graphene Oxide on the Growth and Photosynthesis of the Emergent Plant Iris pseudacorus
Authors: Zhou Zixin , Li Jiaxin , Li Cui , Guo Qiang , Hou Xincun , Zhao Chunqiao , Wang Yu , Chen Chuansheng , Wang Qinghai
Published: April 2023

The extensive applications of graphene oxide (GO) inevitably lead to entry into the natural aquatic environment. However, information on its toxicity to emergent plants is still lacking. In this study, an emergent plant, Iris pseudacorus, was exposed to GO (1, 20, 80, and 140 mg·L−1) under hydroponic conditions for 15 weeks. Changes in plant growth were assessed by analyzing plant biomass and photosynthetic pigment contents; the photosynthesis response was verified by measuring chlorophyll a fluorescence; and the nutrient levels of the plant were evaluated. Results showed that GO at 20–140 mg·L−1 significantly increased plant dry weight by 37–84% and photosynthetic pigment contents by 26–178%, and 80 mg·L−1 was the optimal concentration. PSII activity, adjustment capacities of electron transport in PSII, the grouping or energetic connectivity between PSII units, light energy conversion efficiency, photosynthesis performance indexes (by 11–51%), and contents of several nutrient elements (N, Fe, and Cu) were increased by 49–69%, 34–84%, and 11–38%, respectively. These findings indicate that GO can enhance plant growth by promoting plant photosynthesis performance and improving plant nutrient levels, and has great application potential in promoting the growth and development of this emergent plant as a phytoremediation agent.
Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in Nicotiana tabacum
Authors: Liu Wanhong , Ji Xue , Cao Hanping , Huo Chunsong , He Linshen , Peng Xiang , Yang Ya , Yang Fang , Xiong Shu
Published: April 2023

Mineral nutrition plays an important role in crop growth, yield and quality. MiR156 is a regulatory hub for growth and development. To date, the understanding of miR156-mediated mineral homeostasis is limited. In this study, we overexpressed Nta-miR156a in the tobacco cultivar TN90 and analyzed the effects of miR156 on mineral element homeostasis in tobacco by comparative transcriptome analysis. The results showed that the overexpression of miR156a caused significant morphological changes in transgenic tobacco. Chlorophyll and three anti-resistance markers, proline, total phenolics, and total flavonoids, were altered due to increased miR156 expression levels. Interestingly, the distribution of Cu, Mn, Zn, and Fe in different tissues of transgenic tobacco was disordered compared with that of the wild type. Comparative transcriptome analysis showed that the overexpression of miR156 resulted in 2656 significantly differentially expressed genes. The expression levels of several metal-transport-related genes, such as NtABC, NtZIP, NtHMA, and NtCAX, were significantly increased or decreased in transgenic tobacco. These results suggest that miR156 plays an essential role in regulating mineral homeostasis. Our study provides a new perspective for the further study of mineral nutrient homeostasis in plants.
Experimental Study on Potential Influence of the Invasive Hedychium coronarium J. König on the Evapotranspiration of Riparian Plant Community
Authors: Vergne Driélli de Carvalho , Rosalem Lívia Malacarne Pinheiro , Wendland Edson Cezar , Anache Jamil Alexandre Ayach , Martins da Silva Márcia Cristina , Boschi Raquel Stucchi , Silva Matos Dalva Maria da
Published: April 2023

The balance between precipitation and evapotranspiration (ET) has direct effect on vegetation, and any change in its structure and composition can influence it. The aim of this study is to determine experimentally the daily evapotranspiration (ET) of the invasive species, Hedychium coronarium, and to compare with a group of four native species of the riparian forest. The experiment was carried out in a greenhouse with three different treatments: (1) only the invasive species; (2) only native species; and (3) a mixture of invasive and native species. In each lysimeter, pressure transducers recorded the water level at every 15 min along 14 months. Daily ET was calculated by the method of Gribovszki et al. (2008) and varied according to the treatment, indicating that different species (invasive or native) use the water differently. The maximum accumulated daily ET occurred for mixture treatment (2540.16 mm), while the treatment with the invasive plant presented the lowest value (2172.53 mm). H. coronarium, in monodominant stands, can reduce evapotranspiration on invaded areas and increase it when immersed in the riparian forest.
Influence of Lyophilization and Cryoprotection on the Stability and Morphology of Drug-Loaded Poly(ethylene glycol-b-ε-caprolactone) Micelles
Authors: Hussain Md. Saddam , Faisal Khandokar Sadique , Clulow Andrew J. , Albrecht Hugo , Krasowska Marta , Blencowe Anton
Published: April 2023

Polymeric micelles are promising carriers for the delivery of poorly water-soluble drugs, providing enhanced drug solubility, blood circulation times, and bioavailability. Nevertheless, the storage and long-term stability of micelles in solution present challenges requiring the lyophilization and storage of formulations in the solid state, with reconstitution immediately prior to application. Therefore, it is important to understand the effects of lyophilization/reconstitution on micelles, particularly their drug-loaded counterparts. Herein, we investigated the use of β-cyclodextrin (β-CD) as a cryoprotectant for the lyophilization/reconstitution of a library of poly(ethylene glycol-b-ε-caprolactone) (PEG-b-PCL) copolymer micelles and their drug-loaded counterparts, as well as the effect of the physiochemical properties of different drugs (phloretin and gossypol). The critical aggregation concentration (CAC) of the copolymers decreased with increasing weight fraction of the PCL block (fPCL), plateauing at ~1 mg/L when the fPCL was >0.45. The blank (empty) and drug-loaded micelles were lyophilized/reconstituted in the absence and presence of β-CD (9% w/w) and analyzed via dynamic light scattering (DLS) and synchrotron small-angle X-ray scattering (SAXS) to assess for changes in aggregate size (hydrodynamic diameter, Dh) and morphology, respectively. Regardless of the PEG-b-PCL copolymer or the use of β-CD, the blank micelles displayed poor redispersibility (<10% relative to the initial concentration), while the fraction that redispersed displayed similar Dh to the as-prepared micelles, increasing in Dh as the fPCL of the PEG-b-PCL copolymer increased. While most blank micelles displayed discrete morphologies, the addition of β-CD or lyophilization/reconstitution generally resulted in the formation of poorly defined aggregates. Similar results were also obtained for drug-loaded micelles, with the exception of several that retained their primary morphology following lyophilization/reconstitution, although no obvious trends were noted between the microstructure of the copolymers or the physicochemical properties of the drugs and their successful redispersion.
Flexible Electrochromic Device on Polycarbonate Substrate with PEDOT:PSS and Color-Neutral TiO2 as Ion Storage Layer
Authors: Johannes Christopher , Macher Sven , Niklaus Lukas , Schott Marco , Hillmer Hartmut , Hartung Michael , Heim Hans-Peter
Published: April 2023

Electrochromic (EC) windows on glass for thermal and glare protection in buildings, often referred to as smart (dimmable) windows, are commercially available, along with rearview mirrors or windows in aircraft cabins. Plastic-based applications, such as ski goggles, visors and car windows, that require lightweight, three-dimensional (3D) geometry and high-throughput manufacturing are still under development. To produce such EC devices (ECDs), a flexible EC film could be integrated into a back injection molding process, where the films are processed into compact 3D geometries in a single automized step at a low processing time. Polycarbonate (PC) as a substrate is a lightweight and robust alternative to glass due to its outstanding optical and mechanical properties. In this study, an EC film on a PC substrate was fabricated and characterized for the first time. To achieve a highly transmissive and colorless bright state, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) was used as the working electrode, while titanium dioxide (TiO2) was used as the counter electrode material. They were deposited onto ITO-coated PC films using dip- and slot-die coating, respectively. The electrodes were optically and electrochemically characterized. An ECD with a polyurethane containing gel electrolyte was investigated with regard to optical properties, switching speed and cycling behavior. The ECD exhibits a color-neutral and highly transmissive bright state with a visible light transmittance of 74% and a bluish-colored state of 64%, a fast switching speed (7 s/4 s for bleaching/coloring) and a moderately stable cycling behavior over 500 cycles with a decrease in transmittance change from 10%to 7%.
Free Vibration Responses of Functionally Graded CNT-Reinforced Composite Conical Shell Panels
Authors: Cho Jin-Rae
Published: April 2023

Functionally graded CNT (carbon nanotube)-reinforced composites (FG-CNTRCs) are intensively studied because the mechanical behaviors of conventional composites can be dramatically improved. Only a small amount of CNTs are appropriately distributed through the thickness. However, the studies on conical shell panels have been poorly reported when compared with beams, plates and cylindrical shells, even though more parameters are associated with the mechanical behaviors of conical shell panels. In this context, this study intends to profoundly investigate the free vibration of FG-CNTRC conical shell panels by developing an effective and reliable 2-D (two-dimensional) numerical method. The displacement field is expressed using the first-order shear deformation shell theory, and it is approximated by the 2-D planar natural element method (NEM). The conical shell surface is transformed into the 2-D planar NEM grid, and the approach for MITC3+shell element is employed to suppress the shear locking. The developed numerical method is validated through the benchmark experiments, and the free vibration responses of FG-CNTRC conical shell panels are investigated with respect to all the associated parameters. It is found from the numerical results that the free vibration of FG-CNTRC conical shell panels is significantly influenced by the volume fraction and distribution pattern of CNTs, the geometry parameters of the conical shell, and the boundary condition.
NMR and GPC Analysis of Alkyd Resins: Influence of Synthesis Method, Vegetable Oil and Polyol Content
Authors: Hadzich Antonella , Flores Santiago , Masucci Ashley E. , Gomez Enrique D. , Groß G. Alexander
Published: April 2023

Alkyd resins are oil-based polymers that have been widely used for generations in the surface coating industry and beyond. Characterization of these resins is of high importance to understand the influence of its components on its behavior, compatibility with other resins, and final quality to ensure high durability. Here, NMR spectroscopy and GPC were used for characterizing differences in the chemical structure, molecular distribution, and dispersity between oil-based and fatty acid-based alkyd polymers made from sacha inchi and linseed oils. Sancha inchi (Plukentia volubilis L.) is a fruit-bearing plant native to South America and the Caribbean, and has a rich unsaturated fatty acid content. The effect of vegetable oil and polyol selection on the synthesis of alkyd resins for coating applications was analyzed. The influence of two different synthesis methods, monoglyceride and fatty acid processes, was also compared. Important structural differences were observed using NMR: one-dimensional spectra revealed the degree of unsaturated fatty acid chains along the polyester backbone, whereas, 2D NMR experiments facilitated chemical shift assignments of all signals. GPC analysis suggested that alkyd resins with homogeneous and high molecular weights can be obtained with the fatty acid process, and that resins containing pentaerythritol may have uniform chain lengths.
The Kirkwood–Riseman Model of Polymer Solution Dynamics Is Qualitatively Correct
Authors: Phillies George David Joseph
Published: April 2023

The Rouse model is the foundational basis of much of modern polymer physics. The period alternative, the Kirkwood–Riseman model, is rarely mentioned in modern monographs. The models are qualitatively different. The models do not agree as to how many internal modes a polymer molecule has. In the Kirkwood–Riseman model, polymers in a shear field perform whole-body rotation; in the Rouse model, polymers respond to shear with an affine deformation. We use Brownian dynamics to show that the Kirkwood–Riseman model for chain motion is qualitatively correct. Contrary to the Rouse model, in shear flow, polymer coils rotate. Rouse modes are cross-correlated. The amplitudes and relaxation rates of Rouse modes depend on the shear rate. Several alternatives to Rouse modes as collective coordinates are discussed.
Defect Passivation Scheme toward High-Performance Halide Perovskite Solar Cells
Authors: Du Bin , He Kun , Zhao Xiaoliang , Li Bixin
Published: April 2023

Organic-inorganic halide perovskite solar cells (PSCs) have attracted much attention in recent years due to their simple manufacturing process, low cost, and high efficiency. So far, all efficient organic-inorganic halide PSCs are mainly made of polycrystalline perovskite films. There are transmission barriers and high-density defects on the surface, interface, and grain boundary of the films. Among them, the deep-level traps caused by specific charged defects are the main non-radiative recombination centers, which is the most important factor in limiting the photoelectric conversion efficiency of PSCs devices to the Shockley-Queisser (S-Q) theoretical efficiency limit. Therefore, it is imperative to select appropriate passivation materials and passivation strategies to effectively eliminate defects in perovskite films to improve their photovoltaic performance and stability. There are various passivation strategies for different components of PSCs, including interface engineering, additive engineering, antisolvent engineering, dopant engineering, etc. In this review, we summarize a large number of defect passivation work to illustrate the latest progress of different types of passivators in regulating the morphology, grain boundary, grain size, charge recombination, and defect density of states of perovskite films. In addition, we discuss the inherent defects of key materials in carrier transporting layers and the corresponding passivation strategies to further optimize PSCs components. Finally, some perspectives on the opportunities and challenges of PSCs in future development are highlighted.
Some Logarithmic Intuitionistic Fuzzy Einstein Aggregation Operators under Confidence Level
Authors: Rahman Khaista , Hezam Ibrahim M. , Božanić Darko , Puška Adis , Milovančević Miloš
Published: April 2023

The objective of this paper is to introduce some new logarithm operational laws for intuitionistic fuzzy sets. Some structure properties have been developed and based on these, various aggregation operators, namely confidence logarithmic intuitionistic fuzzy Einstein weighted geometric (CLIFEWG) operator, confidence logarithmic intuitionistic fuzzy Einstein ordered weighted geometric (CLIFEOWG) operator, confidence logarithmic intuitionistic fuzzy Einstein hybrid geometric (CLIFEHG) operator, confidence logarithmic intuitionistic fuzzy Einstein weighted averaging (CLIFEWA) operator, confidence logarithmic intuitionistic fuzzy Einstein ordered weighted averaging (CLIFEOWA) operator, confidence logarithmic intuitionistic fuzzy Einstein hybrid averaging (CLIFEHA) operator have been presented. To show the validity and the superiority of the proposed operators, we compared these methods with the existing methods and concluded from the comparison and sensitivity analysis our proposed techniques are more effective.
A Pilot-Scale Nanofiltration–Ultrafiltration Integrated System for Advanced Drinking Water Treatment: Process Performance and Economic Analysis
Authors: Chen Fengxia , Zhu Lifang , Tang Jianzhong , Li Dongfeng , Yu Fang , Bai Fuqing , Ye Zhou , Cao Lu , Geng Nan
Published: April 2023

In this pilot study, the performance of an “ultrafiltration (UF) + nanofiltration (NF)” advanced treatment process in improving drinking water quality was investigated. The membrane performance and effluent qualities of three commercial NF membranes (Dow Filmtec NF270-400, VONTRON TAPU-LS, and GE Osmonics-HL8040F 400) were evaluated, and the reasons for the difference in effluent quality of these three NF membranes were analyzed. The results showed that UF as a pretreatment process could provide NF with stable and qualified influent. After passing through the UF unit, the turbidity of raw water decreased by 88.6%, and the SDI value was less than 3. Due to the small pore size of NF membranes, organics and polyvalent ions in raw water were further removed. With a water recovery of 90%, the conductivity, chemical oxygen demand (CODMn), and hardness of NF effluent are significantly improved. The three commercial NF membranes showed different performance advantages. Among them, Dow Filmtec NF270-400 had the best desalting performance, VONTRON TAPU-LS had the highest retention rate of organic matter, and GE Osmonics-HL8040F 400 had significantly advanced softening performance. Thanks to the combination of the UF membrane and NF membrane, membrane fouling was effectively inhibited, and drug consumption was within an acceptable range. The operation costs of these three NF membranes were 0.165, 0.179, and 0.171 USD per ton of produced water, respectively. The results showed that the UF + NF process is an ideal technology for advanced treatment in water plants.
Bifurcation Analysis of a Rotor-Casing Coupling System with Bolted Flange Connection under the Effect of Rotor-Casing Rubbing Fault
Authors: Zhu Zhimin , Wen Chuanmei , Long Tianliang , Jin Long , Li Yuqi
Published: April 2023

This study mainly investigated the nonlinear vibration performance of a rotor-casing coupling system containing a bolted flange connection. The dynamic equations of the coupling system were developed while considering the radial stiffness of the bolted flange structure, which contained a spigot, squirrel cage with ball bearing, and rotor-casing coupling vibration. To study the influence of the disk casing fixed-point rubbing fault on the coupling system’s nonlinear dynamic performance, an analytical model of the nonlinear impact forces was established, which considered the contact and vibration responses of the rotor and casing. The frictional force was obtained based on the Coulomb friction law. The iterative analysis of motion equations was performed utilizing the Newmark method. Then, the nonlinear dynamic behaviors of the coupled systems were examined using data, including a bifurcation diagram, spectrum plot, greatest Lyapunov exponents, etc. The effects of rubbing fault on the dynamic properties of system were investigated in detail, indicating that there were various motion states, which were described as periodic, multi-periodic, and quasi-periodic motions. Comparing the simulation results, it was found that rubbing fault seriously affected the motion stability of the rotor system. Finally, by gathering and examining the vibration data set from a test platform for rotor-casings with bolted joints, the correctness of the numerical simulation findings was confirmed. Additionally, the results of the experimental investigation agreed with that of the simulation. The dynamic distinguishing characteristics that were noticed can be used as an indicator for determining whether the fixed-point rubbing fault between the rotor and casing has become worse.
Comparative Analysis of Three WEEE Management Scenarios Based on LCA Methodology: Case Study in the Municipality of Iasi, Romania
Authors: Ghiga Simona Cecilia , Simion Isabela Maria , Filote Cătălina , Roșca Mihaela , Hlihor Raluca Maria , Gavrilescu Maria
Published: April 2023

The increasing consumption of electrical and electronic equipment (EEE), correlated with the fast innovation pace in this field, generates a large amount of annual waste. The current established management practices cannot keep up with it, and the results are of increased significance given the negative effects on the environment and human health. Thus, the current study aimed to analyze the environmental impact of three different scenarios of waste electrical and electronic equipment (WEEE) management, following population awareness campaigns regarding its collection in the Municipality of Iasi, Romania. Data processing was carried out considering Life Cycle Assessment (LCA) methodology with the established functional unit for each scenario according to the collected amount. The results were quantified using the CML2001 and ReCiPe methods and showed that the highest environmental impact was obtained for scenario II (S2) (1.59 × 10−7 pers. equiv. using the CML2001 method and 32.7 pers. equiv. using the ReCiPe method), while the lowest for scenario I (S1) (6.42 × 10−8 pers. equiv. using the CML2001 method and 13.8 pers. equiv. using the ReCiPe method). The process with the highest contribution to the total environmental impact was the collection stage for all scenarios, with the exception of scenario S2, in which case the highest value was generated for the landfill process following the application of the ReCiPe method (39.93%). The current study provides value to a critical issue in the environmental area and supports the development of sustainable WEEE management processes.