bluuebunny/crossref_metadata_2025 huggingface dataset:
RANCANG BANGUN CRM BERBASIS WEB PADA UD. TIMBUL REZEKI
Authors: Khoirani Fahriza , Efendi Bachtiar , Sembiring Muhammad Ardiansyah
Published: May 2023

Abstrack: UD. Timbul Rezeki, located on Jalan Sei Alim Hasak, Sei Dadap District, Asahan Regency, North Sumatra Province is one of the businesses engaged in the business of selling building materials such as cement, paint, nails, wood and other building materials. In carrying out selling and ordering activities, the goods are still done manually and also not computerized, such as customers buying building materials by coming directly to UD. Rezeki arises or by calling the telephone number of UD. Sustenance arises to place an order for building materials. This makes it difficult for customers to know the quality of the building materials available at UD. Sustenance arises. The purpose of this study is to improve customer service at UD. Sustenance arises by implementing Customer Relationship Management. The method used in this research is to apply the method of Customer Relationship Management (CRM). The results of implementing Customer Relationship Management can improve service to customers at UD. Sustenance arises by providing information about products, account registration, product orders, media comments and product ratings, chats, coupon vouchers, discounts, and product catalogs as product promotion media. Keywords: CRM; Sales; Building Materials; Web.  Abstrak: UD. Timbul Rezeki berlokasi Jalan Sei Alim Hasak Kecamatan Sei Dadap, Kabupaten Asahan, Provinsi Sumatera Utara merupakan salah satu usaha bisnis yang bergerak di bidang usaha penjualan material bahan bagunan seperti semen, cat, paku, kayu dan bahan-bahan bangunan lainnya. Dalam melakukan aktivitas menjual dan pemesanan barangnya masih dilakukan secara manual dan juga belum terkomputerisasi seperti pelanggan melakukan pembelian bahan-bahan bangunan dengan datang langsung ke UD. Timbul Rezeki atau dengan menghubungi nomor telepon pihak UD. Timbul Rezeki untuk melakukan pemesanan bahan bagunan. Hal ini yang membuat pelanggan sulit mengetahui kualitas bahan-bahan bangunan yang ada di UD. Timbul Rezeki. Tujuan penelitian ini adalah Untuk meningkatkan pelayanan pelanggan pada UD. Timbul Rezeki dengan menerapkan Customer Relationship Management. Adapun metode yang digunakan dalam penelitian ini adalah dengan menerapkan metode Customer Relationship Management (CRM). Hasil menerapkan menerapkan Customer Relationship Management dapat meningkatkan pelayanan terhadap pelangg:an di UD. Timbul Rezeki dengan memberikan informasi tentang produk, pendaftaran akun, pemesanan produk, media komentar dan rating produk, chatting, voucher kupon, diskon, serta catalog produk sebagai media promosi produk. Kata Kunci : CRM; Penjualan; Material Bahan Bangunan; Web.
PENERAPAN METODE AHP PENENTUAN PENERIMA BANTUAN INDONESIA PINTAR PADA SDN 010069 PUNGGULAN
Authors: Wita Dwi Agus , Siagian Yessica , Rohminatin Rohminatin
Published: May 2023

Abstract: In 2014 the government created the Smart Indonesia Program (PIP) which became part of the improvement of Assistance for Poor Students. PIP is assistance from the government for the poor in terms of education provided to families with school-age children (6-21 years) coming from poor families. Public Elementary School 010069 Punggulan Village is obliged to register students who have KIP with Dapodik so that they can immediately receive PIP benefits. The obstacle faced by SDN 010069 Punggulan Village is that there are many criteria that must be taken into account so that it is difficult to determine the priority of potential PIP recipients. To overcome this problem, it is necessary to have a system to determine the priority of potential PIP recipients by adding some more basic criteria. In this study a decision support system was designed using the AHP (Analitycal Hierarchy Process) method with several criteria, namely Student Active Status, Poor Certificate, Orphan Condition, Parents' Salary, Attendance Presentation. In this application, the final result is the priority ranking of students who will receive PIP assistance. Based on these problems, it can be concluded that there are many criteria for poor students who do not have KIP, making it difficult for SDN 010069 Punggulan Village to determine the priorities of poor students who wish to be registered as PIP recipients. Keywords: PIP Assistance Recipient; AHP Method; PHP and MySQL.  Abstrak: Tahun 2014 pemerintah membuat Program Indonesia Pintar (PIP) yang menjadi bagian penyempurnaan Bantuan Siswa Miskin. PIP merupakan  bantuan  dari pemerintah untuk masyarakat miskin dalam hal pendidikan yang diberikan kepada keluarga dengan anak usia sekolah (6-21 tahun) berasal dari keluarga miskin. Pihak Sekolah  Dasar Negeri 010069 Desa Punggulan berkewajiban untuk mendaftarkan siswanya yang mempunyai KIP ke Dapodik supaya bisa segera mendapat manfaat PIP. Kendala yang dihadapi oleh pihak SDN 010069 Desa Punggulan adalah terdapat banyak kriteria yang harus diperhitungkan sehingga kesulitan dalam menentukan prioritas calon penerima PIP. Untuk mengatasi permasalahan tersebut perlu adanya sistem dalam menentukan prioritas calon penerima PIP dengan menambahkan beberapa kriteria yang  lebih  mendasar. Pada penelitian ini dirancang sistem pendukung keputusan menggunakan metode AHP (Analitycal Hierarchy Process) dengan beberapa kriteria yaitu Status Aktif Siswa, Surat Keterangan  Miskin, Kondisi Yatim Piatu,  Gaji Orang Tua, Presentasi Absensi. Pada aplikasi  ini didapatkan hasil akhir berupa perankingan prioritas siswa  yang  akan  menerima  bantuan PIP. Berdasarkan permasalahan tersebut maka dapat diambil kesimpulan bahwa banyaknya kriteria bagi siswa miskin yang tidak memiliki KIP, menyulitkan pihak SDN 010069 Desa Punggulan dalam menentukan prioritas siswa miskin yang ingin didaftarkan sebagai penerima PIP. Kata kunci : Penerima Bantuan PIP; Metode AHP; PHP dan MySQL
PERAMALAN PERSEDIAAN VOUCHER INTERNET DENGAN METODE SINGLE SINGLE EXPONENTIAL SMOOTHING
Authors: Lubis Mustapa Husein , Tanjung Akhir Abadi , Martina Desy
Published: May 2023

Abstract: Forecasting is predicting something that will happen using past data. Research conducted on the Preza Data package on internet voucher inventory. To make it easier for stores to predict internet inventory, a forecasting technique is used which by using this technique the company can predict the amount of internet voucher inventory for the future. The internal voucher inventory forecasting system uses the Single Exponential Smoothing method. The exponential smoothing method is used in order to see the amount of internet voucher inventory for the future by using the latest data to reduce past data irregularities. The data used to calculate forecasting uses internet voucher inventory data in 2019. From the test results it was found that this forecasting ability was very good because it had a MAPE value of less than 20%. At the sample test stage, it was found that the forecast for the number of internet voucher supplies in March 2021 was 1479 with an error rate of 3.89%. This forecasting has actually been used because the MAPE value is less than 20%. Keywords: Forecasting; Internet Voucher Inventory; Single Exponential Smoothing.  Abstrak: Peramalan adalah memperkirakan sesuatu yang akan terjadi dengan menggunakan data-data masa lalu.. Penelitian di lakukan pada Preza paket Data tentang jumlah persediaan voucher internet. Untuk mempermudah toko dalam memprediksi persediaan voucher internet maka digunakanlah suatu teknik peramalan yang mana dengan menggunakan teknik ini perusahaan dapat memprediksi jumlah persediaan voucher internet untuk masa yang akan datang. Sistem peramalan persediaan voucher internemenggunakan  metode Single Exponential Smoothing. Metode exponential smoothing digunakan agar bisa mengetahui jumlah persediaan voucher untuk masa yang akan datang dengan menggunakan data terbaru untuk mengurangi ketidakteraturan data masa lampau.  Data yang digunakan untuk menghitung peramalan menggunakan data persediaan voucher internet tahun 2019. Dari hasil pengujian didapat bahwa kemampuan peramalan ini sangat baik karena memiliki nilai MAPE kurang dari 20%. Pada tahap uji sample didapat bahwa peramalan jumlah persediaan voucher internet bulan Maret 2021 berjumlah 1479 dengan tingkat error adalah  3,89 %. Peramalan ini sudah aktual digunakan karna nilai MAPE kurang dari 20% Kata kunci: Peramalan; Persediaan Voucher Internet; Single Exponential Smoothing.
PENERAPAN METODE AHP PADA PENENTUAN PRIORITAS PROYEK AIR BERSIH DI KABUPATEN ASAHAN
Authors: Aulia Dewi , Siddik Mohd , Latiffani Chitra
Published: May 2023

Abstract: Asahan Regency is one of the 33 regencies/cities in North Sumatra Province. Coastal areas are still lagging behind because they are far from urban areas so that the government pays little attention to it, especially regarding clean water. Clean water in the area can be said to be unfit for use. So that people find it difficult to get clean water for drinking, washing and bathing. These problems can be solved by programs to improve clean water infrastructure and facilities, such as drilling wells and allocating river water, so that they can provide optimal services for clean water, which has a very strong relationship with the economic growth of a region and the socio-cultural life of the community. Determining the priority scale for clean water must look at various criteria so as to produce accurate and precise results. Space so as to allow the results to be less accurate and not on target as well as errors in determining which clean water should be prioritized, so that a decision support system is needed that can assist in determining which villages have the right to take precedence in the priority of clean water projects. The method used for the decision support system is AHP method. Keywords: Priority Determination of Clean Water Projects; AHP Method.   Abstrak: Kabupaten Asahan merupakan salah satu kabupaten dari 33 kabupaten/kota yang ada di Provinsi Sumatera Utara. Di wilayah Pesisir masih tertinggal karena letaknya yang jauh dari perkotaan sehingga kurang diperhatikan oleh pemerintah, terutama mengenai air bersih. Air bersih di wilayah tersebut bisa di bilang tidak layak untuk digunakan. Sehingga masyarakat kesulitan untuk mendapatkan air bersih baik itu untuk diminum, menyuci dan mandi. Dengan mempertimbangkan hal tersebut maka perlu diterapkan penentuan skala prioritas yang dapat digunakan sebagai suatu acuan dalam penyusunan program proyek untuk tahun anggaran berikutnya. Penentuan skala prioritas air bersih tersebut harus melihat dari berbagai kriteria sehingga menghasilkan hasil yang akurat dan tepat, selama ini penetuan masih berdasarkan musrembang dari pihak Dinas Pekerjaan Umum dan Penataan Ruang sehingga memungkinkan hasil kurang akurat dan kurang tepat sasaran serta terjadinya kesalahan dalam penentuan air bersih yang harus diutamakan, sehingga diperlukan suatu sistem pendukung keputusan yang dapat membantu dalam menentukan desa mana yang berhak didahulukan dalam prioritas proyek air bersih  Metode yang digunakan untuk sistem pendukung keputusan adalah Metode AHP.  Kata Kunci : Penentuan Prioritas Proyek Air Bersih; Metode AHP.
PENERAPAN METODE NAÏVE BAYES DALAM PENENTUAN GIZI PADA IBU HAMIL DI KELURAHAN BUNGA TANJUNG
Authors: Rahmadani Depy , Handoko Wiwin , Sriutami Fuji , Br.Siagian Khairini Utami
Published: May 2023

Abstract: Nutrition is one of the determinants of the quality of human resources. Food given to pregnant women must contain nutrients as needed, so as to support optimal growth and can prevent malnutrition in pregnant women and also help prevent the occurrence of diseases that can interfere with pregnant women. Determination of good nutrition and malnutrition in pregnant women in the sub-district of Datuk Bandar Timur District, Tanjung Balai City must meet the criteria are age, graphida, body weight, lila, hemoglobin and decision. For pregnant women with normal body weight <25 kg, increased energy intake is needed only after pregnancy to increase the metabolic needs of the mother and the energy needs of the growing fetus. The purpose of this study was to determine good nutrition and malnutrition using the Naïve Bayes method which resulted in a decision on good nutrition and malnutrition in pregnant women. Naive Bayes is a simple probabilistic classifier that calculates a set of probabilities by summing the frequencies and combinations of values from a given dataset. Naïve Bayes produces a Precision value for the positive class of 85%, for the negative class of 86%, the recall value for the positive class is 92%, for the negative class is 75%, the f1-score value for the positive class is 88%, for the negative class is 80%, and 85% for accuracy. Keywords: Machine Learning, Naïve Bayes; Nutritional Status.  Abstract: Gizi merupakan salah satu penentu kualitas sumber daya manusia. Makanan yang diberikan pada ibu hamil harus mengandung zat gizi sesuai kebutuhan, sehingga menunjang pertumbuhan yang optimal dan dapat mencegah kurang gizi pada ibu hamil dan juga membantu mencegah timbulnya penyakit-penyakit yang dapat mengganggu ibu hamil. Penentuan gizi baik dan gizi buruk pada ibu hamil di Kelurahan Bunga Tanjung Kecamatan Datuk Bandar Timur, Kota Tanjung Balai harus memenuhi kriteria yaitu umur, grapida, berat badan, lila, hemoglobin dan keputusan. Untuk ibu hamil dengan berat badan  normal <25  kg, peningkatan  asupan  energi diperlukan  hanya  setelah kehamilan  untuk meningkatkan  kebutuhan  metabolisme yang  dibutuhkan ibu  dan kebutuhan  energi janin yang  sedang  tumbuh. Tujuan penelitian ini untuk penentuan gizi baik dan gizi buruk dengan menggunakan metode naïve bayes yang menghasilkan sebuah keputusan gizi baik dan gizi buruk pada ibu hamil. Naive  Bayes  merupakan  sebuah  pengklasifikasian  probabilistik  sederhana  yang menghitung sekumpulan probabilitas dengan menjumlahkan frekuensi dan kombinasi nilai dari dataset yang diberikan. Naïve Bayes menghasilkan nilai Precision untuk kelas positif 85%, untuk kelas negatif 86%, nilai recall untuk kelas positif 92%, untuk kelas negatif 75%, nilai f1-score untuk kelas positif 88%, untuk kelas negatif sebesar 80%, dan nilai akurasi 85%. Kata Kunci: Machine Learning; Naïve Bayes; Status Gizi.
Identifikasi Telur Cacing Endoparasit Pada Exrata Ayam Kampung (Gallus domesticus) Di Desa Jelantik Kecamatan Jonggat Kabupaten Lombok Tengah
Authors: Hidayat Indra
Published: May 2023

Masalah yang diangkat dalam penelitian ini adalah identifikasi telur cacing endoparasit pada Excreta ayam kampung (gellus domesticus) di desa jelantik kecamatan jonggat kabupaten Lombok tengah. Tujuannya adalah untuk mengetahui telur cacing endoparasit pada Excreta (kotoran) ayam kampung. Hasil penelitian ini diharapkan mampu memberikan informasi secara ilmiah tentang adanya telur cacing endoparasit yang ada pada Excreta ayam kampung sehingga nantinya dapat digunakan sebagai pecegahan di Desa Jelantik. Jenis penelitian ini adalah kuantitatif deskriptif. Teknik penggumpulan data menggunakan tiga metode yaitu metode sidementasi, metode natif dan metode pengapungan. Hasil penelitian ini dari dusun repok bunut terdapat 9 telur cacing dari dari 33 sampel, dusun makam ada 11 telur cacing dari 33 sampel, dan dusun dangah ada 12 telur cacing dari 33 sampel. Sehingga hal tersebut menunjukan bahwa terdapat telur cacing yang menyebabkan ayam mengalami kurang nafsu makan, gangguan pencernaan, dan penurunan berat badan pada unggas ayam.
Resistensi Antibiotik Pada Echerichia coli Yang Diisolasi Dari Daging Ayam Broiler Di Pasar Kebon Roek Kota Mataram
Authors: Dita Dedi Putra , Kholik Kholik
Published: May 2023

Pasar kebon roek merupakan merupakan salah satu pasar yang menyediakan daging ayam segar dari berbagai peternakan pulau lombok. Kondisi sanitasi pasar yang kurang baik dan cara penjualan yang tidak higiene memudahkan berbagai mikroba berkembang dan menjadi sumber penularan ke hewan lain dan manusia di area pasar. Penyebab Resistensi antibiotik berasal dari pemakaian antibiotik yang tidak rasional dan pola rantai pangan asal hewan maupun lingkungan. Tujuan penelitian ini untuk mengisolasi Escherichia coli dan mengetahui sensitivitas antibiotik Tetracycline, Gentamicin dan Penicillin G terhadap bakteri tersebut. Penelitian ini dilaksanakan pada 13 Juni 2022, pengambilan sampel dilakukan dipasar kebon roek kota mataram, sedangkan pemeriksaan sampel dilakukan Balai Laboratorium Kesehatan Pengujian dan Kalibrasi Kota Mataram. Penelitian ini menggunakan 8 daging ayam broiler yang diambil di 6 pedagang. Sampel kemudian dimasukkan ke dalam cool box yang sudah diisi es batu. Sampel yang sudah dihaluskan dan dihomogen cairan pgbp kemudian dimasukkan dalam BHI dan diinkubasi selama 24 jam. Sampel lalu ditanam ke Blood Agar dan diinkubasi selama 24 jam pada suhu 37˚C pada inkubator dan dilakukan pewarnaan Gram dan dikarakterisasi uji Biokimia dan dianalisis berdasarkan Bergey's manual of determinative bacteriology (Holt et al, 1994). Selanjutnya dilakukan pengujian resistensi menggunakan antibiotik yaitu Tetracycline, Gentamicin, Penicillin G. Hasil menunjukkan resistensi terhadap antibiotik yaitu Tetracycline sensitif (20%) terhadap E. coli, intermediet sebanyak 2 sampel (40%), dan resisten sebanyak 2 sampel (40%). Gentamicin sensitif (60%), intermediet(20%), dan diperoleh resisten terhadap E. coli, Penisilin G sensitif (0,0%), intermediet sebanyak 1 sampel (20%) dan resisten (80%) di Pasar Kebon Roek Kota Mataram.
THE LENGTH AND THE STRUCTURE OF THE COPY TEXT OF SHEVCHENKO’S POEM “THE GREAT VAULT”
Authors: Boron Oleksandr
Published: May 2023

stands in Subotiv village...” to the text of Shevchenko’s poem “Th e Great Vault”. Stanislav Rosovetskyi provided a detailed criticism of this decision in his time, and his counterarguments are consistently considered in the paper. Most of them are refuted or questioned, false statements and inaccurate information are corrected.

As a result, the following vision of the history of poem’s creation and circulation has been substantiated. In the early October 1845 in Myrhorod, Shevchenko completed the poem “The Great Vault”, the text of which still might not have had an epigraph and epilogue. Probably, a later copy that belonged to Ivan Lazarevskyi indirectly comes from this autograph. While in Maryinske the poet added an epigraph and on October 21 added the epilogue “There stands in Subotiv village...”, marking the date and place of creation. At that time, according to Olexandr Afanasiev-Chuzhbynskyi, whom the poet met in Lubny on October 25 and stayed with in Iskivtsi on October 25—27, the mystery play had no name. After returning to Kyiv, in approximately April — June 1846 Shevchenko rewrote “The Great Vault” along with other works from an unknown autograph to the manuscript collection “Three Years” in such a way that the epilogue of the poem could be mistaken for a separate work, as it was misunderstood by Vasyl Bilozerskyi who copied the mystery without the final fragment. Obviously, the same mistake was made by other copyists, who started the tradition of “Th e Great Vault” without the epilogue. When Shevchenko received an imperfect copy of I. Lazarevskyi, he had no choice but to edit only what it contained.

Apparently, he did not object to the fact that in the copy of Lev Zhemchuzhnykov the final fragment was called “Subotiv”. It is suggested to increase the distance between the end of the third part of the poem and the beginning of the epilogue in the next academic edition, as well as to reproduce all the graphics of the poetic texts in editions closer to the autographs.
Gaelic Community Development and theGàidhealtachdQuestion
Authors: Lewin Christopher
Published: May 2023

The present article discusses issues relating to territorially-differentiated community language development policy in the Scottish Gaelic and Irish contexts, and evaluates recent contributions to debate on the topic. The historical backdrop to current provision for Gaelic community development is explored within the wider context of Gaelic governance structures and funding streams. The article analyses current developments in the Scottish Government’s policymaking process in relation to Gaelic, including proposals relating to the establishment of a geographical Gàidhealtachd, and argues in favour of a new framework for Gaelic community development based on the model of Ireland’s Gaeltacht Act (2012). This would address both the ‘heartland’ and urban contexts, create a more localised level of language planning with greater community control and input, and establish the principle that Gaelic community development should be an elaborated policy area in its own right, with a clear statutory basis.
The Partisan Dimensions of Earmarking in the U.S. House of Representatives
Authors: Fagan E.J. , Theriault Sean
Published: May 2023

Passing out federal dollars to specific projects in particular districts had been a popular way for congressional leaders to build large bipartisan majorities for major bills since the very first meeting of Congress during the Washington administration. By matching projects to districts, scholars have been able to systematically analyze which members were successful in getting their project funded. But for one year, though, the requests members submitted were never known. Using earmark requests data during the 111th Congress, we can know both the requests and the awards. Knowing the former changes our understanding of the latter. We find that the request process is largely driven by ideology, rather than electoral vulnerability. More moderate Republicans tended to seek more earmarks than their more conservative partisans; some of whom completely opted out of the process. The effect was the opposite for Democrats, whose moderates requested fewer earmarks.
Evaluation of integrated pest management based techniques against insect pests associated with tomato at Uthal Balochistan, Pakistan
Authors: Magsi Zahida , Memon Shafique Ahmed , Ali Arif , Baloch Dost Muhammad
Published: May 2023

Insect pests are soft-bodied and tiny creatures with plant sap sucking habits that devastate various agricultural crops, fruit trees, ornamental plants and several vegetables. To control these insect pests four IPM techniques were used in tomato field with an aim to minimize the use of synthetic pesticides. Five colours of sticky traps (yellow, green, blue, black and red), Five different colours light trap (red, blue, yellow, green and white), three botanical insecticides (bitter Apple, tobacco, and neem seed extract) with 30% concentration and three synthetic insecticides (emmamectin benzoate, profenophos and bifenthrin) along with water as a control were used. A maximum pest population of jassid, whitefly, thrips, leaf minor, psyllid and flea beetle was observed on yellow sticky traps, while a minimum pest population of jassid, whitefly, thrips, leaf minor, psyllid and flea beetle was noticed on black and red sticky trap. However a highest pest population of fruit borer, psyllid, blister beetle, asparagus beetle, white fly, flea beetle and fruit fly was observed on white and yellow light colour as compared with others lights. However, no statistically significant differences were observed in jassid and earwings pest population on all given colure of lights. The minimum decreased population of white fly, Jassid, thrips and aphids was observed on all three inorganic pesticides as compared with all three organic pesticides and control group. Similarly pest populations reductions  of  white white fly, Jassid, thrips and aphid was found on all three organic pesticides neem seed, tobacco and bitter apple as compared to control group. We concluded that use of IPM techniques may be encouraged to suppress the insect pest population of tomato crop which could be ultimately positive for the environment
Assessment of effect of fluoride pollution on germination rate, vigor index and fluoride contents in some selected varieties of wheat crop (Triticum aestivum L.)
Authors: Dad Khaliq , Zhao Fenliang , Zhao Fenliang , Iftikhar Sumiyya , Khan Manal Nadeem , Ali Fatima , Fatima Iqra , Nawab Momal , Khan Rania Mazhar , Majid Khansa , Bilal Khola , Nawaz Muhammad
Published: May 2023

Wheat being staple food and a source of economy in Pakistan is cultivated on large scale every year. Due to increasing environmental pollution, this crop is being affected directly or indirectly.  Fluoride is one of the most toxic pollutants among rest of the environmental pollutants. A pot experiment was conducted to examine the impacts of fluoride toxicity on the seed germination, seedlings growth and F ion accumulation in locally cultivated varieties of wheat crop. In this regards, 5 wheat varieties (Akbar 2019, Anaj 2017, Ujala 2016, Galaxy 2016 and AARI 2011) were sown in green house at Bahauddin Zakaryia University (BZU), Multan, Pakistan by applying five (3 mM, 5mM, 7mM, 9mM and 11mM) treatments of NaF and control (0mM). Germination percentage, vigor index and F accumulation in roots and shoots were considered as parameters of research. Anaj 2017 and Akbar 2019 were exhibiting the highest germination percentage at 3mM (87% and 75%) while at 11 mM only Akbar 2019 showed 13% of germination rate as compared to that of control and rest of varieties. Vigor index was also found maximum 193.3 at 11mM of NaF in Anaj 2017. The fluoride contents were recorded higher in roots as compared to that of shoots in all varieties of wheat i.e. at 11 mM, 1 ug of F was found in AARI 2011 which was observed higher as compared to that of control as well as rest of varieties.
Experiencias significativas de formación no formal co niños en el departamento del Tolima
Authors: Sánchez Prieto Fabián Ernesto
Published: May 2023

El presente artículo evalúa algunas experiencias significativas con niños y niñas en territorio, recoge aspectos importantes de la ejecución y puesta en marcha del programa “Generación Explora”, atendiendo a la necesidad de visibilizar los procesos llevados a cabo durante la implementación en los distintos puntos de atención, que fueron priorizados y focalizados por presentar un alto estado de vulnerabilidad y marginación social, limitando las posibilidades para la construcción de un proyecto de vida que les permita superar dicha condición. Es por ello, que, por medio de escenarios participativos de encuentro, se brindó elementos educativos para la promoción del desarrollo de los niños y niñas, a través de la ejecución de metodologías innovadoras y experienciales que les permiten explorar la creatividad, fortalecer habilidades y capacidades, como también la apropiación de conceptos para el descubrimiento de sus talentos.
Acute cannabidiol treatment enhances social interaction in adult male mice
Authors: Ferreira Livia F. , Pathapati Nikhita , Schultz Stephen T. , Nunn Mary C. , Pierce Bethany L. , Sanchez Yatzil R. , Murrell Meredith D. , Ginsburg Brett C. , Onaivi Emmanuel S. , Gould Georgianna G.
Published: May 2023

Cannabidiol (CBD) is a non-intoxicating phytochemical from Cannabis sativa that is increasingly used to manage pain. The potential for CBD to ameliorate dimensional behavior symptoms occurring in multiple psychiatric disorders was suggested, including social interaction impairments. To test this hypothesis, adult male BTBRT+Itpr3tf/J (BTBR) mice, a model of idiopathic autism exhibiting social preference deficits and restrictive repetitive behaviors, were acutely treated with vehicle or 0.1, 1, or 10 mg/kg CBD. Social interaction preference was assessed 50 min after treatment, followed by social novelty preference at 60 min, marble burying at 75 min and social dominance at 120 min. CBD (10 mg/kg) enhanced BTBR social interaction but not social novelty preference, marble burying or dominance, with serum levels = 29 ± 11 ng/mg at 3 h post-injection. Next, acute 10 mg/kg CBD was compared to vehicle treatment in male serotonin transporter (SERT) knock-out mice, since SERT deficiency is an autism risk factor, and in their wildtype background strain controls C57BL/6J mice. CBD treatment generally enhanced social interaction preference and attenuated social novelty preference, yet neither marble burying nor dominance was affected. These findings show acute treatment with as little as 10 mg/kg purified CBD can enhance social interaction preference in male mice that are otherwise socially deficient.
Engineered combinatorial cell device for wound healing and bone regeneration
Authors: Kadunc Polajnar Lucija , Lainšček Duško , Gašperšič Rok , Sušjan-Leite Petra , Kovačič Uroš , Butinar Miha , Turk Boris , Jerala Roman , Hafner-Bratkovič Iva
Published: May 2023

Growth factors are the key regulators that promote tissue regeneration and healing processes. While the effects of individual growth factors are well documented, a combination of multiple secreted growth factors underlies stem cell–mediated regeneration. To avoid the potential dangers and labor-intensive individual approach of stem cell therapy while maintaining their regeneration-promoting effects based on multiple secreted growth factors, we engineered a “mix-and-match” combinatorial platform based on a library of cell lines producing growth factors. Treatment with a combination of growth factors secreted by engineered mammalian cells was more efficient than with individual growth factors or even stem cell–conditioned medium in a gap closure assay. Furthermore, we implemented in a mouse model a device for allogenic cell therapy for an in situ production of growth factors, where it improved cutaneous wound healing. Augmented bone regeneration was achieved on calvarial bone defects in rats treated with a cell device secreting IGF, FGF, PDGF, TGF-β, and VEGF. In both in vivo models, the systemic concentration of secreted factors was negligible, demonstrating the local effect of the regeneration device. Finally, we introduced a genetic switch that enables temporal control over combinations of trophic factors released at different stages of regeneration mimicking the maturation of natural wound healing to improve therapy and prevent scar formation.
Comparison of metagenomes from fermentation of various agroindustrial residues suggests a common model of community organization
Authors: Myers Kevin S. , Ingle Abel T. , Walters Kevin A. , Fortney Nathaniel W. , Scarborough Matthew J. , Donohue Timothy J. , Noguera Daniel R.
Published: May 2023

The liquid residue resulting from various agroindustrial processes is both rich in organic material and an attractive source to produce a variety of chemicals. Using microbial communities to produce chemicals from these liquid residues is an active area of research, but it is unclear how to deploy microbial communities to produce specific products from the different agroindustrial residues. To address this, we fed anaerobic bioreactors one of several agroindustrial residues (carbohydrate-rich lignocellulosic fermentation conversion residue, xylose, dairy manure hydrolysate, ultra-filtered milk permeate, and thin stillage from a starch bioethanol plant) and inoculated them with a microbial community from an acid-phase digester operated at the wastewater treatment plant in Madison, WI, United States. The bioreactors were monitored over a period of months and sampled to assess microbial community composition and extracellular fermentation products. We obtained metagenome assembled genomes (MAGs) from the microbial communities in each bioreactor and performed comparative genomic analyses to identify common microorganisms, as well as any community members that were unique to each reactor. Collectively, we obtained a dataset of 217 non-redundant MAGs from these bioreactors. This metagenome assembled genome dataset was used to evaluate whether a specific microbial ecology model in which medium chain fatty acids (MCFAs) are simultaneously produced from intermediate products (e.g., lactic acid) and carbohydrates could be applicable to all fermentation systems, regardless of the feedstock. MAGs were classified using a multiclass classification machine learning algorithm into three groups, organisms fermenting the carbohydrates to intermediate products, organisms utilizing the intermediate products to produce MCFAs, and organisms producing MCFAs directly from carbohydrates. This analysis revealed common biological functions among the microbial communities in different bioreactors, and although different microorganisms were enriched depending on the agroindustrial residue tested, the results supported the conclusion that the microbial ecology model tested was appropriate to explain the MCFA production potential from all agricultural residues.
Down-regulation of β-lactam antibiotics resistance and biofilm formation by Staphylococcus epidermidis is associated with isookanin
Authors: Ren Qiang , Luo Wanhe , Chi Haoming , Zhang Lili , Chen Wei
Published: May 2023

Introduction

Biofilm formation is the major pathogenicity of Staphylococcus epidermidis (S. epidermidis), which enhances bacterial resistance to antibiotics. Isookanin has potential inhibitory activity on biofilm.

Method

The inhibiting mechanisms of isookanin against biofilm formation through surface hydrophobicity assay, exopolysaccharides, eDNA, gene expression analysis, microscopic visualization, and molecular docking were explored. Additionally, the combination of isookanin and β-lactam antibiotics were evaluated by the broth micro-checkerboard assay.

Results

The results showed that isookanin could decrease the biofilm formation of S. epidermidis by ≥85% at 250 μg/mL. The exopolysaccharides, eDNA and surface hydrophobicity were reduced after treatment with isookanin. Microscopic visualization analysis showed that there were fewer bacteria on the surface of the microscopic coverslip and the bacterial cell membrane was damaged after treatment with isookanin. The down-regulation of icaB and up-regulation of icaR were observed after treatment with isookanin. Additionally, the RNAIII gene was significantly up-regulated (p < 0.0001) at the mRNA level. Molecular docking showed that isookanin could bind to biofilm-related proteins. This indicated that isookanin can affect biofilm formation at the initial attachment phase and the aggregation phase. The FICI index showed that the combination of isookanin and β-lactam antibiotics were synergistic and could reduce doses of antibiotics by inhibiting biofilm formation.

Discussion

This study improved the antibiotic susceptibility of S. epidermidis through inhibition of the biofilm formation, and provided a guidance for the treatment of antibiotic resistance caused by biofilm
The resource (in)sufficiency of the Caribbean: analyzing socio-metabolic risks (SMR) of water, energy, and food
Authors: Martin del Campo Francisco , Singh Simron Jit , Mijts Eric
Published: May 2023

Introduction

Socio-metabolic risks (SMRs) are systemic risks associated with the availability of critical resources, the integrity of material circulation, and the distribution of their costs and benefits in a socio-ecological system. For resource-stressed systems like small island nations, understanding trade-offs and synergies between critical resources is not only crucial, but urgent. Climate change is already putting small islands at high risk through more frequent and intense extreme weather events, changing precipitation patterns, and threats of inundation with future sea-level rise.

Methods

This study compares the shifting resource-baseline for 14 Caribbean island nations for the year 2000 and 2017. We analyze water, energy, and food (WEF) and their nexus through the lens of SMRs, using indicators related to their availability, access, consumption, and self-sufficiency.

Results

Our findings point to the decreasing availability of all three resources within the Caribbean region. Meanwhile, between 2000 and 2017, consumption levels have increased by 20% with respect to water (from 230 to 275 m3/cap/yr) and primary energy (from 89 to 110 GJ/cap/yr), and 5% for food (from 2,570 to 2,700 kcal/cap/day). While universal access to these resources increased in the population, food and energy self-sufficiency of the region has declined.

Discussion

Current patterns of resource-use, combined with maladaptive practices, and climate insensitive development—such as coastal squeeze, centralized energy systems, and trade policies—magnify islands' vulnerability. Disturbances, such as climate-induced extreme events, environmental changes, financial crises, or overexploitation of local resources, could lead to cascading dysfunction and eventual breakdown of the biophysical basis of island systems. This research is a first attempt at operationalizing the concept of SMRs, and offers a deeper understanding of risk-related resource dynamics on small islands, and highlights the urgency for policy response.
Chronic anemia is associated with systemic endothelial dysfunction
Authors: Chennupati Ramesh , Solga Isabella , Wischmann Patricia , Dahlmann Paul , Celik Feyza Gül , Pacht Daniela , Şahin Aslıhan , Yogathasan Vithya , Hosen Mohammad Rabiul , Gerdes Norbert , Kelm Malte , Jung Christian
Published: May 2023

Background

In acute myocardial infarction and heart failure, anemia is associated with adverse clinical outcomes. Endothelial dysfunction (ED) is characterized by attenuated nitric oxide (NO)-mediated relaxation responses which is poorly studied in chronic anemia (CA). We hypothesized that CA is associated with ED due to increased oxidative stress in the endothelium.

Methods

CA was induced by repeated blood withdrawal in male C57BL/6J mice. Flow-Mediated Dilation (FMD) responses were assessed in CA mice using ultrasound-guided femoral transient ischemia model. Tissue organ bath was used to assess vascular responsiveness of aortic rings from CA mice, and in aortic rings incubated with red blood cells (RBCs) from anemic patients. In the aortic rings from anemic mice, the role of arginases was assessed using either an arginase inhibitor (Nor-NOHA) or genetic ablation of arginase 1 in the endothelium. Inflammatory changes in plasma of CA mice were examined by ELISA. Expression of endothelial NO synthase (eNOS), inducible NO synthase (iNOS), myeloperoxidase (MPO), 3-Nitrotyrosine levels, and 4-Hydroxynonenal (4-HNE) were assessed either by Western blotting or immunohistochemistry. The role of reactive oxygen species (ROS) in ED was assessed in the anemic mice either supplemented with N-Acetyl cysteine (NAC) or by in vitro pharmacological inhibition of MPO.

Results

The FMD responses were diminished with a correlation to the duration of anemia. Aortic rings from CA mice showed reduced NO-dependent relaxation compared to non-anemic mice. RBCs from anemic patients attenuated NO-dependent relaxation responses in murine aortic rings compared to non-anemic controls. CA results in increased plasma VCAM-1, ICAM-1 levels, and an increased iNOS expression in aortic vascular smooth muscle cells. Arginases inhibition or arginase1 deletion did not improve ED in anemic mice. Increased expression of MPO and 4-HNE observed in endothelial cells of aortic sections from CA mice. NAC supplementation or inhibition of MPO improved relaxation responses in CA mice.

Conclusion

Chronic anemia is associated with progressive endothelial dysfunction evidenced by activation of the endothelium mediated by systemic inflammation, increased iNOS activity, and ROS production in the arterial wall. ROS scavenger (NAC) supplementation or MPO inhibition are potential therapeutic options to reverse the devastating endothelial dysfunction in chronic anemia.
Estimated plasma volume status: association with congestion, cardiorenal syndrome and prognosis in precapillary pulmonary hypertension
Authors: Yogeswaran Athiththan , Richter Manuel J. , Husain-Syed Faeq , Rako Zvonimir , Sommer Natascha , Grimminger Friedrich , Seeger Werner , Ghofrani Hossein Ardeschir , Gall Henning , Tello Khodr
Published: May 2023

Background

Volume overload is often associated with clinical deterioration in precapillary pulmonary hypertension (PH). However, thorough assessment of volume overload is complex and therefore not routinely performed. We examined whether estimated plasma volume status (ePVS) is associated with central venous congestion and prognosis in patients with idiopathic pulmonary arterial hypertension (IPAH) or chronic thromboembolic PH (CTEPH).

Methods

We included all patients with incident IPAH or CTEPH enrolled in the Giessen PH Registry between January 2010 and January 2021. Plasma volume status was estimated using the Strauss formula.

Results

In total, 381 patients were analyzed. Patients with high ePVS (≥4.7 vs. <4.7 ml/g) at baseline showed significantly increased central venous pressure (CVP; median [Q1, Q3]: 8 [5, 11] mmHg vs. 6 [3, 10] mmHg) and pulmonary arterial wedge pressure (10 [8, 15] mmHg vs. 8 [6, 12] mmHg), while right ventricular function was not altered. In multivariate stepwise backward Cox regression, ePVS was independently associated with transplant-free survival at baseline and during follow-up (hazard ratio [95% confidence interval]: 1.24 [0.96, 1.60] and 2.33 [1.49, 3.63], respectively). An intra-individual decrease in ePVS was associated with a decrease in CVP and predicted prognosis in univariate Cox regression. Patients with high ePVS without edema had lower transplant-free survival than those with normal ePVS without edema. In addition, high ePVS was associated with cardiorenal syndrome.

Conclusions

In precapillary PH, ePVS is associated with congestion and prognosis. High ePVS without edema may represent an under-recognized subgroup with poor prognosis.
Transcatheter aortic valve durability: a contemporary clinical review
Authors: Montarello Nicholas J. , Willemen Yannick , Tirado-Conte Gabriela , Travieso Alejandro , Bieliauskas Gintautas , Sondergaard Lars , De Backer Ole
Published: May 2023

Encouraged by randomized controlled trials demonstrating non-inferiority of transfemoral transcatheter aortic valve implantation (TAVI) compared to surgical aortic valve replacement (SAVR) across all surgical risk categories, there has been a dramatic increase in the use of TAVI in a younger patient cohort with severe aortic stenosis, endorsed by both European and American Cardiac Societies. However, the standard use of TAVI in younger, less co-morbid patients with a longer life expectancy can only be supported if there is sound data demonstrating long-term durability of transcatheter aortic valves (TAVs). In this article, we have reviewed available randomized and observational registry clinical data pertaining to TAV long-term durability, placing emphasis on trials and registries using the new standardized definitions of bioprosthetic valve dysfunction (BVD) and bioprosthetic valve failure (BVF). Despite inherent difficulties in interpreting the available data, the determination reached is that the risk of structural valve deterioration (SVD) is potentially lower after TAVI than SAVR at 5 to 10 years, and that the two treatment modalities have a similar risk of BVF. This supports the adoption of TAVI in younger patients evident in current practice. However, the routine use of TAVI in younger patients with bicuspid aortic valve stenosis should be cautioned due to insufficient long-term TAV durability data in this particular patient population. Finally, we highlight the importance of future research into the unique potential mechanisms that can potentially contribute to TAV degeneration.
Development of “LvL UP 1.0”: a smartphone-based, conversational agent-delivered holistic lifestyle intervention for the prevention of non-communicable diseases and common mental disorders
Authors: Castro Oscar , Mair Jacqueline Louise , Salamanca-Sanabria Alicia , Alattas Aishah , Keller Roman , Zheng Shenglin , Jabir Ahmad , Lin Xiaowen , Frese Bea Franziska , Lim Chang Siang , Santhanam Prabhakaran , van Dam Rob M. , Car Josip , Lee Jimmy , Tai E Shyong , Fleisch Elgar , von Wangenheim Florian , Tudor Car Lorainne , Müller-Riemenschneider Falk , Kowatsch Tobias
Published: May 2023

Background

Non-communicable diseases (NCDs) and common mental disorders (CMDs) are the leading causes of death and disability worldwide. Lifestyle interventions via mobile apps and conversational agents present themselves as low-cost, scalable solutions to prevent these conditions. This paper describes the rationale for, and development of, “LvL UP 1.0″, a smartphone-based lifestyle intervention aimed at preventing NCDs and CMDs.

Materials and Methods

A multidisciplinary team led the intervention design process of LvL UP 1.0, involving four phases: (i) preliminary research (stakeholder consultations, systematic market reviews), (ii) selecting intervention components and developing the conceptual model, (iii) whiteboarding and prototype design, and (iv) testing and refinement. The Multiphase Optimization Strategy and the UK Medical Research Council framework for developing and evaluating complex interventions were used to guide the intervention development.

Results

Preliminary research highlighted the importance of targeting holistic wellbeing (i.e., both physical and mental health). Accordingly, the first version of LvL UP features a scalable, smartphone-based, and conversational agent-delivered holistic lifestyle intervention built around three pillars: Move More (physical activity), Eat Well (nutrition and healthy eating), and Stress Less (emotional regulation and wellbeing). Intervention components include health literacy and psychoeducational coaching sessions, daily “Life Hacks” (healthy activity suggestions), breathing exercises, and journaling. In addition to the intervention components, formative research also stressed the need to introduce engagement-specific components to maximise uptake and long-term use. LvL UP includes a motivational interviewing and storytelling approach to deliver the coaching sessions, as well as progress feedback and gamification. Offline materials are also offered to allow users access to essential intervention content without needing a mobile device.

Conclusions

The development process of LvL UP 1.0 led to an evidence-based and user-informed smartphone-based intervention aimed at preventing NCDs and CMDs. LvL UP is designed to be a scalable, engaging, prevention-oriented, holistic intervention for adults at risk of NCDs and CMDs. A feasibility study, and subsequent optimisation and randomised-controlled trials are planned to further refine the intervention and establish effectiveness. The development process described here may prove helpful to other intervention developers.
The early animal radiation: insights from interpreting the Cambrian problematic fossils
Authors: Cong Peiyun
Published: May 2023

Genic and genomic data have been reshaping our understanding of the earliest radiation event of metazoans, the well-known Cambrian Evolutionary Radiation, not only from the respects of reshuffling the phylogenetic topologies of some animal phyla but by deciphering the deep homologies of many morphological features. These advances, together with the continuing discoveries of the Ediacaran-Cambrian fossils, are unveiling the cladogenetic process of the early metazoans and the patterns of morphologic evolution during this biological radiation event. In this review, I focus on a small but challenging field, the problematic fossils from the early Cambrian fossil Lagerstätten, such as the Chengjiang biota, mainly on the controversies concerning their interpretation and the consequent impacts on understanding the early evolution of animals. The bizarre body plans of the early Cambrian problematica alone do not account for the difficulties in studying their biology and affinity. Instead, it is the combined action of the taphonomic artifacts and the uncertainty in homologizing the preserved characters that impede generating plausible interpretations. Despite all these issues, a testable and repeatable method for interpreting fossils has emerged and is becoming more practicable. The integration of an evolutionary-grade conceptual frame is beneficial to the interpretation of the Cambrian problematic fossils. Together with the focus on taphonomic alternation and homologic assessment, the Cambrian problematic fossils are becoming more informative nodes in the “parsing tree” of early animal evolution.