bluuebunny/crossref_metadata_2025 huggingface dataset:
Telomere length in granulosa cells and leukocytes: a potential marker of female fertility? A systematic review of the literature
Authors: Fattet Anne-Julie , Toupance Simon , Thornton Simon N. , Monnin Nicolas , Guéant Jean-Louis , Benetos Athanase , Koscinski Isabelle
Published: August 2020

In the context of a continuously increased delay of motherhood and of an increase of the incidence of premature ovarian failure, it is of the greatest interest to dispose of a predictive marker of the duration of the fertility window. Unfortunately, current available markers of women’s fertility (hormonal rates or echography count of small follicles) have a poor predictive value of premature ovarian failure. In the last ten years, some studies have suggested that telomere length may be correlated with premature ovarian failure, but the results of these studies are contradictory.

In accordance with guidelines from Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), this systematic review of the literature selected studies evaluating telomere length or telomerase activity in granulosa cells and/or in leukocytes as a premature ovarian failure marker.

Five publications (252 premature ovarian failure patients) were included in this review of experimental evidence. Two of them studied telomere length and/or telomerase activity in granulosa cells and 4 in leukocytes in women with premature ovarian failure. For each study, authors determined if there was a positive or a negative correlation between telomeric parameters and premature ovarian failure.

3 studies (178 premature ovarian failure patients) found shorter telomere length in granulosa cells and/or leukocytes and/or lower telomerase activity in premature ovarian failure patients. 2 studies (74 premature ovarian failure patients) presented contradictory results about the correlation of leucocyte telomere length with premature ovarian failure.

Shorter telomeres and diminished telomerase activity in granulosa cells appear to be associated with ovarian insufficiency. However, the number of studies and of subjects within are low and the methodology questionable. The confirmation of these results is essential with more subjects, better defined populations and more adapted methodology, in order to consider telomere length in granulosa cells and/or in leucocytes as an early and reliable marker for the decline of ovarian function.
Gene co-expression and histone modification signatures are associated with melanoma progression, epithelial-to-mesenchymal transition, and metastasis
Authors: Azevedo Hátylas , Pessoa Guilherme Cavalcante , de Luna Vitorino Francisca Nathália , Nsengimana Jérémie , Newton-Bishop Julia , Reis Eduardo Moraes , da Cunha Júlia Pinheiro Chagas , Jasiulionis Miriam Galvonas
Published: August 2020

Background

We have previously developed a murine cellular system that models the transformation from melanocytes to metastatic melanoma cells. This model was established by cycles of anchorage impediment of melanocytes and consists of four cell lines: differentiated melanocytes (melan-a), pre-malignant melanocytes (4C), malignant (4C11−), and metastasis-prone (4C11+) melanoma cells. Here, we searched for transcriptional and epigenetic signatures associated with melanoma progression and metastasis by performing a gene co-expression analysis of transcriptome data and a mass-spectrometry-based profiling of histone modifications in this model.

Results

Eighteen modules of co-expressed genes were identified, and some of them were associated with melanoma progression, epithelial-to-mesenchymal transition (EMT), and metastasis. The genes in these modules participate in biological processes like focal adhesion, cell migration, extracellular matrix organization, endocytosis, cell cycle, DNA repair, protein ubiquitination, and autophagy. Modules and hub signatures related to EMT and metastasis (turquoise, green yellow, and yellow) were significantly enriched in genes associated to patient survival in two independent melanoma cohorts (TCGA and Leeds), suggesting they could be sources of novel prognostic biomarkers. Clusters of histone modifications were also linked to melanoma progression, EMT, and metastasis. Reduced levels of H4K5ac and H4K8ac marks were seen in the pre-malignant and tumorigenic cell lines, whereas the methylation patterns of H3K4, H3K56, and H4K20 were related to EMT. Moreover, the metastatic 4C11+ cell line showed higher H3K9me2 and H3K36me3 methylation, lower H3K18me1, H3K23me1, H3K79me2, and H3K36me2 marks and, in agreement, downregulation of the H3K36me2 methyltransferase Nsd1.

Conclusions

We uncovered transcriptional and histone modification signatures that may be molecular events driving melanoma progression and metastasis, which can aid in the identification of novel prognostic genes and drug targets for treating the disease.
CFDSD: a Communication Framework for Distributed Software Development
Authors: L’Erario Alexandre , Gonçalves José Antônio , Fabri José Augusto , Pagotto Tiago , Cunha Palácios Rodrigo Henrique
Published: August 2020

Due to geographical and/or temporal dispersion, communication between teams in distributed software projects is a critical factor for success. Notably, distributed teams suffer adverse physical and temporal dispersion effects during an information exchange. To mitigate problems arising from interactions, it is important to understand the communication structure of teams during distributed projects. The objective of this work is to present the Communication Framework for Distributed Software Development. This framework groups a set of distributed projects communication concepts and enables a unified view of all stakeholders’ intercommunications that use many interaction technologies. The goal of this framework is to portray the dynamics of the interaction between distributed teams in a multi-tier structure, and each tier approaches a single function in the intercommunication process. We analyzed all interactions from distributed teams after developing an experimental IT Project, using the content analysis method to validate the Communication Framework for Distributed Software Development. The main contribution of this work is the framework specification, and the investigation, which has the potential to help mapping communication patterns in DSD. Moreover, this framework comprises interfaces for communication assessment and includes intercommunications from automated engineering tools as bots.
Visual short-term memory relates to tau and amyloid burdens in preclinical autosomal dominant Alzheimer’s disease
Authors: Norton Daniel J. , Parra Mario A. , Sperling Reisa A. , Baena Ana , Guzman-Velez Edmarie , Jin David S. , Andrea Nicholas , Khang Juna , Schultz Aaron , Rentz Dorene M. , Pardilla-Delgado Enmanuelle , Fuller Joshua , Johnson Keith , Reiman Eric M. , Lopera Francisco , Quiroz Yakeel T.
Published: August 2020

Background

Over the past decade, visual short-term memory (VSTM) binding tests have been shown to be one of the most sensitive behavioral indicators of Alzheimer’s disease (AD), especially when they require the binding of multiple features (e.g., color and shape). Recently, it has become possible to directly measure amyloid and tau levels in vivo via positron emission tomography (PET). To this point, these behavioral and neurochemical markers have not been compared in humans with AD or at risk for it.

Methods

In a cross-sectional study, we compared VSTM performance to tau and amyloid concentrations, measured by PET, in individuals certain to develop AD by virtue of their inheritance of the presenilin-1 E280A mutation. These included 21 clinically unimpaired subjects and 7 subjects with early mild cognitive impairment (MCI), as well as 30 family members who were not carriers of the mutation.

Results

We found that VSTM performance correlated strongly with tau in entorhinal cortex and inferior temporal lobe, and also with amyloid when examining asymptomatic carriers only. The condition requiring binding was not preferentially linked to tau—in fact, the non-binding “shape only” condition showed a stronger relationship.

Conclusions

The results confirm VSTM’s status as an early marker of AD pathology and raise interesting questions as to the course of binding-specific versus non-binding aspects of VSTM in early AD.
Sex differences in circulating proteins in heart failure with preserved ejection fraction
Authors: Stienen Susan , Ferreira João Pedro , Kobayashi Masatake , Preud’homme Gregoire , Dobre Daniela , Machu Jean-Loup , Duarte Kevin , Bresso Emmanuel , Devignes Marie-Dominique , Andrés Natalia López , Girerd Nicolas , Aakhus Svend , Ambrosio Giuseppe , Rocca Hans-Peter Brunner-La , Fontes-Carvalho Ricardo , Fraser Alan G. , van Heerebeek Loek , de Keulenaer Gilles , Marino Paolo , McDonald Kenneth , Mebazaa Alexandre , Papp Zoltàn , Raddino Riccardo , Tschöpe Carsten , Paulus Walter J. , Zannad Faiez , Rossignol Patrick
Published: August 2020

Background

Many patients with heart failure with preserved ejection fraction (HFpEF) are women. Exploring mechanisms underlying the sex differences may improve our understanding of the pathophysiology of HFpEF. Studies focusing on sex differences in circulating proteins in HFpEF patients are scarce.

Methods

A total of 415 proteins were analyzed in 392 HFpEF patients included in The Metabolic Road to Diastolic Heart Failure: Diastolic Heart Failure study (MEDIA-DHF). Sex differences in these proteins were assessed using adjusted logistic regression analyses. The associations between candidate proteins and cardiovascular (CV) death or CV hospitalization (with sex interaction) were assessed using Cox regression models.

Results

We found 9 proteins to be differentially expressed between female and male patients. Women expressed more LPL and PLIN1, which are markers of lipid metabolism; more LHB, IGFBP3, and IL1RL2 as markers of transcriptional regulation; and more Ep-CAM as marker of hemostasis. Women expressed less MMP-3, which is a marker associated with extracellular matrix organization; less NRP1, which is associated with developmental processes; and less ACE2, which is related to metabolism. Sex was not associated with the study outcomes (adj. HR 1.48, 95% CI 0.83–2.63), p = 0.18.

Conclusion

In chronic HFpEF, assessing sex differences in a wide range of circulating proteins led to the identification of 9 proteins that were differentially expressed between female and male patients. These findings may help further investigations into potential pathophysiological processes contributing to HFpEF.
Bond Behavior Between Steel Fiber Reinforced Polymer (SRP) and Concrete
Authors: Zou Xingxing , Sneed Lesley H.
Published: August 2020

Steel fiber reinforced polymer (SRP) composite materials, which consist of continuous unidirectional steel wires (cords) embedded in a polymeric matrix, have recently emerged as an effective solution for strengthening of reinforced concrete (RC) structures. SRP is bonded to the surface of RC structures by the same matrix to provide external reinforcement. Interfacial debonding between the SRP and concrete is a primary concern in this type of application. This study aimed to investigate the bond characteristics between SRP and concrete determined by single-lap direct shear tests with different composite bonded lengths and fiber sheet densities (cord spacings). Specimens with medium density fibers failed mainly due to composite debonding, whereas those with low density fibers failed due to fiber rupture. Results of specimens that exhibited debonding were used to determine the bond-slip relationship of the SRP-concrete interface and to predict the full-range load response, which was in good agreement with the experimental results. A database of SRP-concrete direct shear tests reported in the literature was also established. Four analytical equations derived for fiber reinforced polymer (FRP)-concrete debonding were evaluated based on the database results and were found to predict the maximum load within approximately 15% error on average, however, they all underestimated the effective bond length.
Impact of a global leader on pharmaceutical practice and policy around the world
Authors: Alves da Costa F. , Henman M. , Hughes C. , Leufkens H. , Babar Z. , McElnay J. , Schulz M.
Published: August 2020

This commentary describes the contributions of a Dutch pharmacist who contributed in a unique manner to the development of community pharmacy practice in Europe, to the evolution of practice-based research and to its publication. With an interest in pharmaceutical care and in clinical pharmacy, Dr. van Mil changed practice and policy in Europe over the last decades in a very visible way, here documented through a summary of some of his main written contributions. We write this to honour his memory and contribute to the preservation of his legacy.
Uniformity, diversity, harmony, and emotional energy in a Chinese STEM classroom
Authors: Davis James P. , Du Juan , Tang Jia-Hui , Qiao Lu , Liu Yan-Qiu , Chiang Feng-Kuang
Published: August 2020

Background

Understanding cultural diversity in STEM education is important for inclusivity and teaching STEM literacy for all. We explore diversity at a micro-cultural classroom level involving students’ emotional experiences and interactional practices as they engage with learning, which has received limited attention in naturalistic studies of STEM education, especially in East Asian cultures. In this study, we explore emotional experiences of students during a guided inquiry lesson in a Chinese primary school using an interpretive methodology informed by concepts of emotional energy, uniformity, and harmony. Emotional energy is an individual and collective experience of togetherness and solidarity emerging from successful social interaction. Uniformity and harmony are emotion-related concepts experienced in Confucian heritage cultures. The aims of this study are to explore Chinese students’ emotional experiences during a STEM inquiry lesson and to refine the conceptualization of emotional energy from Confucian cultural perspectives.

Results

In a STEM inquiry, context knowledge about micro-cultural diversity and the interplay between Chinese emotional experiences and engagement with ideas are explored. Notions of uniformity and harmony are described through cultural practices as they relate to emotional energy. The study contributes to understanding the diversity of emotional energy as a student experience and social form of engagement, during a STEM inquiry activity in a Confucian heritage context.

Conclusions

This study contributes to understandings of student experiences of uniformity, harmony, and variations to intensity of emotional energy during learning via STEM inquiry. Modifications to the conceptualization of emotional energy in STEM education are indicated. Our findings inform an understanding of student emotive experiences of STEM education in East Asian and multi-cultural education systems internationally.
Evaluating the impact of sampling schemes on leaf area index measurements from digital hemispherical photography in Larix principis-rupprechtii forest plots
Authors: Zou Jie , Hou Wei , Chen Ling , Wang Qianfeng , Zhong Peihong , Zuo Yong , Luo Shezhou , Leng Peng
Published: August 2020

Background

Digital hemispherical photography (DHP) is widely used to estimate the leaf area index (LAI) of forest plots due to its advantages of high efficiency and low cost. A crucial step in the LAI estimation of forest plots via DHP is choosing a sampling scheme. However, various sampling schemes involving DHP have been used for the LAI estimation of forest plots. To date, the impact of sampling schemes on LAI estimation from DHP has not been comprehensively investigated.

Methods

In this study, 13 commonly used sampling schemes which belong to five sampling types (i.e. dispersed, square, cross, transect and circle) were adopted in the LAI estimation of five Larix principis-rupprechtii plots (25 m × 25 m). An additional sampling scheme (with a sample size of 89) was generated on the basis of all the sample points of the 13 sampling schemes. Three typical inversion models and four canopy element clumping index (Ωe) algorithms were involved in the LAI estimation. The impacts of the sampling schemes on four variables, including gap fraction, Ωe, effective plant area index (PAIe) and LAI estimation from DHP were analysed. The LAI estimates obtained with different sampling schemes were then compared with those obtained from litter collection measurements.

Results

Large differences were observed for all four variable estimates (i.e. gap fraction, Ωe, PAIe and LAI) under different sampling schemes. The differences in impact of sampling schemes on LAI estimation were not obvious for the three inversion models, if the four Ωe algorithms, except for the traditional gap-size analysis algorithm were adopted in the estimation. The accuracy of LAI estimation was not always improved with an increase in sample size. Moreover, results indicated that with the appropriate inversion model, Ωe algorithm and sampling scheme, the maximum estimation error of DHP-estimated LAI at elementary sampling unit can be less than 20%, which is required by the global climate observing system, except in forest plots with extremely large LAI values (~ > 6.0). However, obtaining an LAI from DHP with an estimation error lower than 5% is impossible regardless of which combination of inversion model, Ωe algorithm and sampling scheme is used.

Conclusion

The LAI estimation of L. principis-rupprechtii forests from DHP was largely affected by the sampling schemes adopted in the estimation. Thus, the sampling scheme should be seriously considered in the LAI estimation. One square and two transect sampling schemes (with sample sizes ranging from 3 to 9) were recommended to be used to estimate the LAI of L. principis-rupprechtii forests with the smallest mean relative error (MRE). By contrast, three cross and one dispersed sampling schemes were identified to provide LAI estimates with relatively large MREs.
Effects of climate, biotic factors, and phylogeny on allometric relationships: testing the metabolic scaling theory in plantations and natural forests across China
Authors: Sun Han , Wang Xiangping , Fan Dayong
Published: August 2020

Background

Metabolic scaling theory (MST) is still in debate because observed allometric exponents often deviate from MST predictions, and can change significantly depending on environment, phylogeny, and disturbance. We assembled published scaling exponents from literatures for three allometric relationships linked to biomass allocation: leaf biomass-diameter (L-D), stem biomass-diameter (S-D), and root biomass-diameter (R-D). We used data from natural forests and plantations across China to test the following hypotheses: 1) the allometric relationships of trees support the predictions of MST on a broad scale; 2) the observed deviations from MST predictions are caused by climate, biotic factors, and/or phylogeny; 3) abiotic and biotic factors influence allometric relationships in plantations and natural forests differently, and different allometric relationships (i.e. L-D, S-D, and R-D) are affected differently. We related these scaling exponents to geographic climate gradient, successional stage, stand density, leaf form and phenology, and phylogeny. We used mixed-effect models to examine the major factors affecting tree allometries.

Results

In natural forests, S-D and R-D scaling exponents were consistent with MST predictions in primary forests, but were significantly lower in secondary forests. Both S-D and R-D scaling exponents in plantations had a medium value that fell between those of the secondary and primary forests, despite plantations being similar in species characteristics and age to secondary forests. The S-D and R-D exponents were significantly affected by factors that are not yet considered in MST, including winter coldness which explained 2.76% – 3.24% of variations, successional stage (7.91% – 8.20% of variations), density (a surrogate for competition, 5.86% – 8.54% of variations), and especially phylogeny (45.86% – 56.64% of variations explained). However, the L-D scaling exponents conformed to MST predictions in primary, secondary, and plantation forests, and was not strongly explained by most factors.

Conclusion

MST is only applicable to primary (steady-state) forests, and climate, biotic factors and phylogeny are causes of the observed deviations of allometric relationships from MST predictions. Forest management practices in plantations have a strong influence on tree allometries. L-D allometry is more strongly controlled by biophysical constraints than S-D and R-D allometries, however, the mechanisms behind this difference still need further examinations.
Should we investigate mitochondrial disorders in progressive adult-onset undetermined ataxias?
Authors: Pedroso José Luiz , de Rezende Pinto Wladimir Bocca Vieira , Barsottini Orlando Graziani Povoas , Oliveira Acary Souza Bulle
Published: August 2020

Background

Despite the broad development of next-generation sequencing approaches recently, such as whole-exome sequencing, diagnostic workup of adult-onset progressive cerebellar ataxias without remarkable family history and with negative genetic panel testing for SCAs remains a complex and expensive clinical challenge.

Case presentation

In this article, we report a Brazilian man with adult-onset slowly progressive pure cerebellar ataxia, which developed neuropathy and hearing loss after fifteen years of ataxia onset, in which a primary mitochondrial DNA defect (MERRF syndrome - myoclonus epilepsy with ragged-red fibers) was confirmed through muscle biopsy evaluation and whole-exome sequencing.

Conclusions

Mitochondrial disorders are a clinically and genetically complex and heterogenous group of metabolic diseases, resulting from pathogenic variants in the mitochondrial DNA or nuclear DNA. In our case, a correlation with histopathological changes identified on muscle biopsy helped to clarify the definitive diagnosis. Moreover, in neurodegenerative and neurogenetic disorders, some symptoms may be evinced later during disease course. We suggest that late-onset and adult pure undetermined ataxias should be considered and investigated for mitochondrial disorders, particularly MERRF syndrome and other primary mitochondrial DNA defects, together with other more commonly known nuclear genes.
Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human
Authors: Martinez Maria Alejandra Frias , Balderrama Ísis de Fátima , Karam Paula Stephania Brandão Hage , de Oliveira Rodrigo Cardoso , de Oliveira Flávia Amadeu , Grandini Carlos Roberto , Vicente Fábio Bossoi , Stavropoulos Andreas , Zangrando Mariana Schutzer Ragghianti , Sant’Ana Adriana Campos Passanezi
Published: August 2020

Purpose

The aim of this study was to investigate the response of osteogenic cell lineage and gingival fibroblastic cells to different surface treatments of grade IV commercially pure Titanium (cpTi) disks.

Material and methods

Grade IV cpTi disks with different surfaces were produced: machined (M), sandblasting (B), sandblasting and acid subtraction (NP), and hydrophilic treatment (ACQ). Surface microtopography characteristics and chemical composition were investigated by scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS). Adhesion and proliferation of SC-EHAD (human surgically-created early healing alveolar defects) and HGF-1 (human gingival fibroblasts) on Ti disks were investigated at 24 and 48 h, and osteogenic differentiation and mineralization were evaluated by assessing alkaline phosphatase (ALP) activity and alizarin red staining, respectively.

Results

No significant differences were found among the various surface treatments for all surface roughness parameters, except for skewness of the assessed profile (Rsk) favoring M (p = 0.035 ANOVA). M disks showed a slightly higher (p > 0.05; Kruskal-Wallis/Dunn) adhesion of HGF-1 (89.43 ± 9.13%) than SC-EHAD cells (57.11 ± 17.72%). ACQ showed a significantly higher percentage of SC-EHAD (100%) than HGF-1 (69.67 ± 13.97%) cells adhered at 24 h. SC-EHAD cells expressed increased ALP activity in osteogenic medium at M (213%) and NP (235.04%) surfaces, but higher mineralization activity on ACQ (54.94 ± 4.80%) at 14 days.

Conclusion

These findings suggest that surface treatment influences the chemical composition and the adhesion and differentiation of osteogenic cells in vitro.

Clinical relevance

Hydrophilic surface treatment of grade IV cpTi disks influences osteogenic cell adhesion and differentiation, which might enhance osseointegration.
An extremely rare case of neuromuscular and vascular hamartoma of the appendix
Authors: Sasaki Takahiro , Furuhata Tomohisa , Nishimura Masashige , Ono Tatsunori , Noda Akiyoshi , Koizumi Hirotaka , Miyajima Nobuyoshi , Otsubo Takehito
Published: August 2020

Background

Neuromuscular and vascular hamartoma is a rare lesion of the small intestine, with only 26 cases reported since its initial description in 1982. No occurrence of hamartoma in the appendix has been reported until now.

Case presentation

A 60-year-old man had been suffering from longstanding right lower quadrant pain. Abdominal computed tomography showed a slight swelling of the appendix as the possible cause of his pain. Laparoscopic appendectomy with partial resection of the cecum was performed for diagnostic and therapeutic purposes. An 18 × 10-mm lesion located on the tip of the appendix was found in the resected specimen. Pathological examination showed that the lesion was covered with normal mucosa and consisted of adipose tissue, smooth muscle fibers, small vessels, and neural fibers. These findings were consistent with neuromuscular and vascular hamartoma of the appendix.

Conclusion

This is the first report of neuromuscular and vascular hamartoma arising from the appendix.
Assessment of exercise-induced stress by automated measurement of salivary cortisol concentrations within the circadian rhythm in Japanese female long-distance runners
Authors: Ushiki Kazumi , Tsunekawa Katsuhiko , Shoho Yoshifumi , Martha Larasati , Ishigaki Hirotaka , Matsumoto Ryutaro , Yanagawa Yoshimaro , Nakazawa Asuka , Yoshida Akihiro , Nakajima Kiyomi , Araki Osamu , Kimura Takao , Murakami Masami
Published: August 2020

Background

Overtraining syndrome, caused by prolonged excessive stress, results in reduced performance and cortisol responsiveness in athletes. It is necessary to collect saliva samples sequentially within circadian rhythm for assessing exercise stress by measuring cortisol concentrations, and automated cortisol measurements using electrochemiluminescence immunoassay (ECLIA) may be useful for measuring a large number of saliva samples. In this study, we evaluated the appropriate use of cortisol-based exercise stress assessment within the circadian rhythm, which may diagnose and prevent overtraining syndrome in athletes.

Methods

We collected saliva and sera from 54 healthy participants and analyzed the correlation between salivary cortisol concentrations measured by ECLIA and enzyme-linked immunosorbent assay (ELISA) or serum cortisol analysis. We also collected saliva continuously from 12 female long-distance runners on 2 consecutive days involving different intensities and types of exercise early in the morning and in the afternoon and measured salivary cortisol concentrations using ECLIA. Each exercise intensity of runners was measured by running velocities, Borg Scale score, and rate of change in the pulse rate by exercise.

Results

ECLIA-based salivary cortisol concentrations correlated positively with those detected by ELISA (ρ= 0.924,p< 0.001) and serum cortisol (ρ= 0.591,p= 0.001). In long-distance runners, circadian rhythm of salivary cortisol, including the peak after waking and the decrease promptly thereafter, were detected on both days by continuous saliva sampling. The rates of change in salivary cortisol concentrations were significantly lower after an early morning exercise than after an afternoon exercise on both days (day 1,p= 0.002, and day 2,p= 0.003). In the early morning exercise, the rate of change in salivary cortisol concentration was significantly higher on day 1 than on day 2 (p= 0.034), similar to a significant difference in running velocities (p= 0.001).

Conclusions

Our results suggest that automated ECLIA-based salivary cortisol measurements are able to detect the athletes’ circadian rhythm and compare the exercise stress intensities at the same times on different days, even in the early morning, possibly leading to the prevention of overtraining syndrome.
Development and feasibility of a tailored habit-based dietary intervention coupled with natural tooth replacement on the nutritional status of older patients
Authors: McCrum Leigh-Ann , Watson Sinead , McGowan Laura , McGuinness Bernadette , Cardwell Christopher , Clarke Mike , Woodside Jayne V. , McKenna Gerry
Published: August 2020

Background

Older adults suffering partial tooth loss may need additional intervention strategies other than natural tooth replacement alone to improve their nutritional status. This study aimed to design and develop a habit-based tailored dietary intervention and to assess the feasibility and acceptability of the intervention, in conjunction with natural tooth replacement, amongst partially dentate older adults.

Methods

The design and development of the dietary intervention (phase 1) consisted of analysis of the target population’s dietary intake and qualitative research through focus groups with community-dwelling older adults (aged 65 years and over). The dietary intervention consisted of forming three healthy dietary habits around fruits and vegetables, wholegrains and healthy proteins. Feasibility of the intervention was then tested amongst older adults who had recently completed dental treatment for natural tooth replacement in a small non-randomised single arm study (phase 2). The principal feasibility outcome was the usability and acceptability of the intervention which was measured using evaluation questionnaires and by conducting post-intervention semi-structured interviews. Supporting outcomes consisted of feasibility of screening procedures, recruitment strategies and retention/attrition rates as well as the participant’s compliance to the intervention assessed through self-monitoring tracking sheets.

Results

Twenty-one older adults (mean [SD] age 72.1 [10.4].) took part in one of four focus group discussions (phase 1). Twelve themes related to barriers (e.g. oral health, appetite) and facilitators (e.g. nutritional knowledge, retirement) of healthy eating guided intervention development, as did a further five themes when asked for direct intervention feedback. Nine older adult participants (mean [SD] age 72.5[9.7]) were recruited into the feasibility study (phase 2) where eight themes were identified from feedback interview discussions. The principal outcome measures identified intervention feasibility as participants considered the intervention acceptable and useable as both the evaluation questionnaire and qualitative interview results were overwhelmingly positive. As a supporting outcome measure, strong intervention compliance was also achieved. Screening procedures were accepted but additional recruitment strategies (e.g. incorporation of home study visits or recruitment via posters advertisement) may benefit future study enrolment and retention.

Conclusion

Phase 1 and phase 2 findings have allowed for an iterative, user-driven intervention to be developed and refined for a randomised control study to evaluate the intervention’s effectiveness.

Trial registration

ISRCTN66118345
Community acquired paediatric pneumonia; experience from a pneumococcal vaccine- naive population
Authors: Kudagammana Sanath Thushara , Karunaratne Ruchira Ruwanthika , Munasinghe Thilini Surenika , Kudagammana Haputhanthirige Donna Wasana Samank
Published: August 2020

Background

Childhood pneumonia continues to be a disease that causes severe morbidity and mortality among children mainly in South-East Asia and Africa though it is not so in the developed world. Pneumonia accounts for 16% of all deaths of children under 5 years old in the world, killing nearly one million children in 2015. In Sri Lanka, there were 21,000 reported cases of pneumonia in 2006, 40% were in the age group of less than 4 years.

Methods

This was a retrospective study done on the children aged 1 month to 14 years who were admitted to the Professorial Paediatric unit of Teaching Hospital, Peradeniya between 1st of March 2016 and 30th of July 2017 fulfilling diagnostic criteria for community-acquired pneumonia. Data including diagnosis, clinical details, management details and other relevant data were collected from patient records by using a data collection sheet.

Results

In this study, 48% of 127 patients admitted with community-acquired pneumonia had bronchopneumonia. About 2/3 of the patients neededa secondline of intravenous antibiotics while 51/ 127 needed care in the high dependency unit with supplemental oxygen. No mortality was observed in the group.

Conclusions

Community- acquired paediatric pneumonia has a significant associated morbidity but not mortality in the studied population. The need for the second-line treatment with intravenous antibiotics in a significant proportion of patients may indicate a high degree of antibiotic resistance. Introduction of national antibiotic policy will help the cause.
Serum 20S proteasome levels are associated with disease activity in MPO-ANCA-associated microscopic polyangiitis
Authors: Maruyama Hiroshi , Hirayama Kouichi , Yamashita Marina , Ohgi Kentaro , Tsujimoto Ryuji , Takayasu Mamiko , Shimohata Homare , Kobayashi Masaki
Published: August 2020

Background

Proteasomes are found in both the cell nucleus and cytoplasm and play a major role in the ubiquitin-dependent and -independent non-lysosomal pathways of intracellular protein degradation. Proteasomes are also involved in the turnover of various regulatory proteins, antigen processing, cell differentiation, and apoptosis. To determine the diagnostic value of serum proteasome in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), we investigated patients with AAV at various stages of the disease.

Methods

Serum 20S-proteasome was measured by ELISA in 44 patients with MPO-ANCA-associated microscopic polyangiitis (MPA) and renal involvement. Thirty of the patients provided serum samples before the initial treatment, and 30 provided samples during remission; 16 provided samples at both time points.

Results

The mean serum 20S-proteasome level was significantly higher in the active-vasculitis patients (3414.6 ± 2738.9 ng/mL; n = 30) compared to the inactive-vasculitis patients (366.4 ± 128.4 ng/mL; n = 30; p <  0.0001) and 40 controls (234.9 ± 90.1 ng/mL; p <  0.0001). There were significant positive correlations between the serum 20S-proteasome level and the Birmingham Vasculitis Activity Score (BVAS) (r = 0.581, p <  0.0001), the ANCA titer (r = 0.384, p <  0.0001), the white blood cell (WBC) count (r = 0.284, p = 0.0042), the platelet count (r = 0.369, p = 0.0002), and the serum C-reactive protein (CRP) level (r = 0.550, p < 0.0001). There were significant negative correlations between the serum 20S-proteasome level and both the hemoglobin concentration (r = − 0.351, p = 0.0003) and the serum albumin level (r = − 0.460, p < 0.0001). In a multiple regression analysis, there was a significant positive correlation between the serum 20S-proteasome level and only the BVAS results (β = 0.851, p = 0.0009). In a receiver operating curve analysis, the area under the curve for the serum 20S-proteasome level was 0.996, which is higher than those of the WBC count (0.738) and the serum CRP level (0.963).

Conclusion

The serum level of 20S-proteasome may be a useful marker for disease activity in AAV.
Mobility and sales activity during the Corona crisis: daily indicators for Switzerland
Authors: Eckert Florian , Mikosch Heiner
Published: August 2020

This paper documents daily compound indicators on physical mobility and sales activity in Switzerland during the Corona crisis. We report several insights from these indicators: The Swiss population substantially reduced its activities already before the shops closed and before the authorities introduced containment policies in mid-March 2020. Activity started to gradually recover from the beginning of April onwards, again substantially before the first phase of the shutdown easing started at the end of April. Low physical mobility during the second half of March and during April likely contributed to the quick fall in new COVID-19 infections since mid-March. The sharp drop in economic activity in consumer-related services during March and April and the gradual recovery in these sectors since May correlate strongly with the reduction and subsequent gradual resurgence of mobility. In addition, while activity within Switzerland was back to normal levels by late June, activity of Swiss residents outside of Switzerland was still below normal.
Occurrence of metastatic eccrine porocarcinoma in an immunosuppressed patient
Authors: Modi Mitul B. , Moshiri Ata S. , Shoji Toru , Mihm Martin C. , Xu Xiaowei , Elder David E.
Published: August 2020

Background

Eccrine porocarcinoma is a rare malignancy accounting for 0.005 to 0.01% of all cutaneous tumors. However, its etiology is not well established. Herein we are reporting the occurrence of metastatic porocarcinoma of the back in a patient with a history of multiple cutaneous malignancies and chronic immunosuppression.

Case presentation

A 79-year-old male with a history of long-term immunosuppressive therapy for left lung transplantation and multiple cutaneous malignancies including melanoma, presented with an enlargening plaque on the right upper back. Biopsy demonstrated an infiltrative epithelial tumor with aggressive histology and ductal formation. Immunohistochemical stains for p63, CK-5/6, and CEA were diffusely positive, while CK-7 and CK-20 were negative. A diagnosis of eccrine porocarcinoma was made. He underwent complete wide excision with negative margins of the right upper back in January 2018. In November 2018, he was found to have a right posterior shoulder mass, positive right axillary lymph nodes, superficial right upper back soft tissue masses and lymphangitic carcinomatosis involving the left lung. He was transitioned to comfort care and subsequently passed from metastatic disease in December 2018.

Conclusion

The occurrence of eccrine porocarcinoma in a patient on long term immunosuppressive therapy for lung transplantation suggests a possible role for chronic immunosuppression in the induction of a subset of eccrine porocarcinomas, as in other cutaneous malignancies.
Hope in a time of civicide: regenerative development and IPAT
Authors: Newman Peter
Published: August 2020

This paper is  written as the world faces economic recovery after the Covid pandemic collapse. It also responds to the article inSustainable Earthby Peter Hancock ‘In Praise of Civicide’ by creating a more hopeful vision of the future. Peter suggests the only hope is in psychological mind-sets that can change behaviour as nothing else will stop the path to destruction from present growth in population, the economy and technology. Rather than seeing inevitable civicidal elements, as devised in the IPAT model used by Peter and many others to explain global environmental destruction, the paper reassesses the fundamentals of this model developed by Paul and Anne Ehrlich in the 1960's. As the global economy has collapsed and environmental impacts improved everywhere, the Hancock argument based on IPAT would seem to have support. The paper shows how it is possible to grow again in the three IPAT factors if the world moves beyond sustainable development which just minimises impact to regenerative development which reclaims environmental impacts. If all three elements combine to create uncontrolled growth as was happening in the 60’s to 80’s then civicide is inevitable, but not if they change to regenerative development. The three stages of exploitive, sustainable and regenerative development turn IPAT from being negative to positive about civilization. These choices are very stark in the 2020’s. The technological possibilities of a regenerative future are outlined and the fundamentals needed for a sustainable earth are sketched, providing some evidence of hope for using the present pandemic and economic collapse as the basis for regenerating civilization not praising civicide.
Hand strenght and dexterity in interphalangeal hand osteoarthritis and effects of osteophyte formations
Authors: Güven Nurhan , Dinçer Fitnat , Çetin Alp , Güven Serdar Can
Published: August 2020

Background

Hand osteoarthritis (HOA) is a complex disorder with various subtypes characterized with predominance of different features. It is challenging to estimate the severity of hand disability in HOA, since contribution of different disease components to clinical burden is yet to be clarified. The aim of the study is to investigate hand functions in nonerosive interphalangeal hand osteoarthritis (HOA) without inflammatory features, and search for effects of osteophyte formations detected by radiography and ultrasound on functionality.

Methods

Thirty one HOA patients and 20 healthy subjects with similar age, gender, body mass index were included. Hand functions were evaluated by self-reported questionnaires and objective strength and dexterity measurements. A total of 459 interphalangeal joints were evaluated and scored by radiography and ultrasound for ostephyte formations.

Results

Strength and dexterity measurements were similar between groups. Self-reported functionality was hampered in HOA group but not statistically significant. Osteophyte scores obtained by ultrasound and radiography were significantly higher in HOA group. Osteophyte scores obtained by ultrasound were higher than the scores obtained by radiography. Ultrasound scores showed no correlation with any of the parameters while osteophytes scores obtained by radiography partially showed a significant negative correlation with assembly part of dexterity testing.

Conclusions

No significant difference observed in hand strength and dexterity in nonerosive interphalangeal HOA patients withouth signs of inflammation when compared to healthy subjects. Osteophyte formations prominent enough to be deteceted by radiography may have a negative effect on hand dexterity.
New freshwater plesiosaurian materials from the Middle Jurassic Xintiangou Formation of the Sichuan Basin, southwestern China
Authors: Zhang Feng , Yu Hai-Dong , Xiong Can , Wei Zhao-Ying , Peng Guang-Zhao , Wei Xue-Fang
Published: August 2020

Sichuan Basin is very famous for the Mesozoic reptiles, especially the Jurassic dinosaurs. Here, we report some isolated plesiosaurian teeth and vertebrae newly excavated from the Middle Jurassic Xintiangou Formation in Yunyang county, Chongqing City, the northeastern region of the Sichuan Basin, southwestern China. The specimens are referred to Pliosauroidea based on the combination of the following features: the circular cross-section of the tooth crown, apicobasal ridges fully covering the enamel surface and reaching to the apex, the short and amphicoelous centrum, the centrum shorter than wide or tall, and the absence of a keel on the lateral surface of the cervical centrum. With the fragmentary nature, it is undetermined whether the Yunyang specimens belong to a known taxon or represent a new species of Plesiosauria. These plesiosaurian specimens further demonstrate that the freshwater plesiosaurians are common in the Jurassic deposits of the Sichuan Basin. For a comprehensive knowledge of the plesiosaurians of the basin in origin, distribution and diversity, to discover new and more complete specimens is necessarily the first priority.
Predicting response to neo-adjuvant chemotherapy and assessment of residual disease in breast cancer using contrast-enhanced spectral mammography: a combined qualitative and quantitative approach
Authors: Kamal Rasha Mohammed , Saad Sherihan Mahmoud , Moustafa Amr Farouk Ibrahim , Gomaa Mohammed Mohammed , Mokhtar Omniya , Gouda Iman , Hassan Ahmed , Hilal Amany , ElZayat Ashraf
Published: August 2020

Background

Magnetic resonance imaging (MRI) is the gold standard imaging modality for evaluation of response for neo-adjuvant chemotherapy (NAC) in breast cancer as it has the advantage of providing both; morphology assessment together with providing functional information which can be obtained by contrast injection. Until the recent emergence of contrast-enhanced mammography as a promising breast imaging modality, these features were considered unique for MRI. The aim of the study is to evaluate the competence of contrast-enhanced spectral mammography (CESM) in the prediction of response to NAC and the assessment of residual disease extent, as well as the assessment of a new combined (quantitative and qualitative) evaluation approach that is proposed by the authors. The study included 81 patients with pathologically proved breast cancer scheduled for receiving NAC. They underwent 2 CESM examinations; pre- and post-NAC (maximum 10 days before surgery). All patients were assessed using the RECIST 1.1 criteria and a combined approach (RECIST+ qualitative subjective assessment). Results were in correlation to postoperative pathology using the Miller-Payne grading. For statistical evaluation, patients were classified into responders and non-responders.

Results

Postoperative histopathology showed that 60/81 lesions were responders (Miller-Payne grades 3, 4, and 5) while the combined response evaluation approach and RECIST 1.1 alone showed 57/60 (95%) patients and 46/60 patients (76.7%) as responders respectively. The combined response evaluation approach showed higher sensitivity and positive and negative predictive values compared to the evaluation based on RECIST alone (95%, 87.6%, and 81.2% compared to 76.6%, 86.7%, and 50% respectively).

Conclusion

CESM can be readily used to assess tumor response to NAC and allows the assessment of functional changes in residual tumor cells in addition to size discrepancy. Using CESM, we could accurately assess residual tumor extent and thus enable better surgical planning.
Large subcapsular hematoma of the liver due to faja corset: a rare case report
Authors: Minhas Sheharyar , Minhas Ahmed , Malik Maira , Sumanam Phaniram
Published: August 2020

Background

Subcapsular hematoma of the liver is a potentially life-threatening but extremely rare condition. It is often caused by a blunt trauma or other predisposing conditions such as a liver tumor, intra-tumor hemorrhage, surgery, preeclampsia, liver biopsy, and hemodialysis. Predisposing causes of liver hematoma include its large size and proximity to fixed structures. To date, there have been no reported cases of subcapsular liver hematoma caused by tight clothing such as corsets. Our case report is about an extremely rare case of subcapsular hematoma of the liver caused by wearing a tight faja corset in a young healthy female.

Case presentation

A forty-five-year-old Spanish female without any underlying health problems presented with sudden onset epigastric and right upper quadrant abdominal pain after wearing a faja corset the night prior to the hospital presentation. CT abdomen was noted for subcapsular hematoma of the liver. Her symptoms persisted and repeat CT abdomen showing worsening of the liver hematoma. Patient had interventional radiology (IR) guided drainage and was subsequently discharged home.

Conclusions

Subscapular liver hematomas need to be considered in patients presenting with acute onset abdominal pain after wearing certain tight clothing. The necessity of an early and accurate diagnosis is vital for management as hemodynamically stable patients can be managed conservatively. Our patient was managed with IR-guided aspiration drainage of the hematoma. Surgery can be considered a last resort in case of life-threatening hemodynamic instability, peritoneal signs, free abdominal fluid, and failure of arterial embolization. Our case highlights the importance of early recognition of traumatic subcapsular hematomas to prevent life-threatening complications.