bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of cyanide and heavy metals removal in liquid effluents from small mining’s gold benefit, by adsorption with activated carbon and hydrogen peroxide in Segovia, Antioquia
Authors: Estrada-Montoya Claudia Catalina , Restrepo Franco Gloria Maria , Galeano Vanegas Narmer Fernando
Published: April 2020

The small gold mining generates toxic substances discharges, being an environmental problem. The objective was to evaluate the removal of cyanide and heavy metals, in liquid effluents from the gold benefit, by adsorption with activated carbon and hydrogen peroxide. The residues were first treated with carbon to determine the adsorption efficiency with 20, 40, 60 g of carbon / L of solution at times of 4, 8, 12 hours. Then hydrogen peroxide (1.0, 1.5, 2.0 liters of peroxide / Kg CN in solution, was added over 4 hours). The response variables were concentrations of cyanide, lead, zinc, iron. The best treatment with carbon was 60 g of carbon / L of solution and 12 hours of contact and for the process with hydrogen peroxide: 2 liters of H2O2 / Kg of CN in solution, during 4 hours. A flow chart and tables for the implementation of the process were designed.
Study of scenarios associated with the development of the MACTOR Agropolis in the Middle Magdalena region of Santander– Colombia
Authors: Castaño Castellanos Jenny Katherine , Becerra ardila Luis Eduardo
Published: April 2020

En este trabajo se presenta un acercamiento a los distintos escenarios de relaciones que se evidencian en cuanto a temas de trabajo colaborativo entre actores que hacen parte del Sistema de Innovación Agroindustrial en la región del Magdalena Medio Santandereano, destacando la importancia que implica para los tomadores decisiones, contar con este tipo de información a la hora de definir planes, programas, proyectos y lineamientos que contribuyan con el fortalecimiento económico, social y ambiental de la región. La metodología utilizada para generar los escenarios que se han obtenido, se ha basado tanto en la búsqueda de literatura como en el acercamiento a actores del sistema y el uso del método de prospectiva MACTOR. Los resultados que se han alcanzado, permiten evidenciar la necesidad de generar mayores esfuerzos de colaboración entre productores, gobiernos, y otras instituciones como la Organización de las Naciones Unidas.
Citral nanocontainers applied to guava fruits (Psidium Guajava L.) in postharvesting
Authors: Macias Socha Claudia Lorena , Reyes Cuellar Julia Constanza
Published: April 2020

The guava (Psidium guajava L.), is a nutritious fruit which is of climacteric nature and highly perishable. This fruit is grown in Santander and Boyacá and 90% of its use is in the agro-based industry. Post-harvest losses of this fruit have a great impact on the economy of the region. In order to prolong the shelf life of the fruit, a post-harvest treatment based on the encapsulation of 3 mM Citral in liposomal nanocontainers composed of 10,12-pentacosadiynoic acid and Lecithin was devised. The characterization of the nanocontainers encapsulating Citral was performed by fluorescence and DLS spectroscopy. The treatment's effectiveness in the fruits was evaluated for 15 days using the following parameters: weight loss, color, total soluble solids, acidity, ripening index, and Vitamin C concentration. The results show a better appearance in the treated fruits with respect to untreated fruits (Blank) for up to 12 days of storage.
Decision-Making Support of Truck Selection: A Systematic Review
Authors: Dos Santos Jesus Liandra , Leal Gislaine Camila Lapasini , Cardoza Galdamez Edwin Vladimir , Lima Junior Francisco Rodrigues
Published: April 2020

Trucks are attached to many industries in different sectors like an industrial equipment. For this reason, select a truck without consider the best attributes for the company could cause futures problems. Therefore, this article presents a systematic review of studies that approach quantitative and qualitative decision-making support methods and attributes that help on collecting of precision data. Thus, a framework was created to describe the factors and characteristics of studies, like the application types, hybrid models, sources and data validation. As result, 11 articles were selected from 893, published in English. And from the accepted articles valuation, multicriteria methods and a quality tool were found both applicated in decision-making support. Also, all the criteria, sub-criteria and attributes applicated by all the 11 authors in the methods were presented in this article and they were classified in 12 factors to simplify them.
The association between sympatho-vagal balance and central blood pressures
Authors: Latchman P. L. , Gates G. , Pereira J. , Axtell R R. , Gardner K. , Schlie J. , Yang Q. , Yue T. , Morin-Viall A. , DeMeersman R.
Published: April 2020

Purpose

High central blood pressure is more predictive of cardiovascular disease (CVD) versus high peripheral blood pressure. Measures of central pressures (CPs) include, central systolic blood pressure (CSBP) and central diastolic blood pressure. Measures of central pressures augmentation (CPsA) include augmentation pressure (AP) and the augmentation index @ 75 beats·min−1 (AIx@75). Increased sympathetic tone (ST) is also associated with CVD. The low to high frequency ratio (LF/HF) is often used to determine sympatho-vagal balance. Given the association between ST, CPs, CPsA and CVD there is a need to understand the association between these predictors of CVD. The aims of this study were to examine the association between the LF/HF ratio, CPs, and CPsA in men and women collectively and based on gender.

Methods

We measured the LF/HF ratio, CSBP, AP, and AIx@75 in 102 participants (41F/61M). The LF/HF ratio was determined via power spectral density analysis. CSBP, AP, and AIx@75 were determined via applanation tonometry.

Results

The LF/HF ratio was inversely associated with AP (r = –0.26) and AIx @75 (r = –0.29) in the combined group of men and women. The LF/HF ratio was inversely associated with CSBP (r = –0.27), AP (r = –0.28), and AIx@75 (r = –0.32) in men, but not in women.

Conclusion

There is an inverse association between the LF/HF ratio, AP, and AIx@75 in men and women combined. The association between the LF/HF ratio, CSBP, AP, and AIx@75, differs based on gender.
The stability of sample storage for complete blood count (CBC) toward the blood cell morphology
Authors: Rahmanitarini Arie , Hernaningsih Yetti , Indrasari Yulia Nadar
Published: April 2020

Background: Peripheral blood smear examination had a pivotal role in determining diagnosis and as confirmation of the automatic hematology analyzer results. The storage process was highly influential on the morphological stability of the cell. This study aimed to assess the morphological changes in blood cells stored at certain period and temperature. Method: Sample of 30 blood specimens of healthy people with dipotassium ethylenediaminetetraacetic acid (K2EDTA) anticoagulants. The specimens were stored at room temperature (18-25ÂºC) and refrigerator temperature (2-8ÂºC) and were analyzed at the preliminary examination i.e., 8 hours, 16 hours, 24 hours, 48 hours, 72 hours, and 96 hours. Kappa test was used in validating the reading of PBS (Peripheral Blood Smear). The discrimination testing used were paired t-test and Kolmogorov Smirnov test with p value <0.005, which stated to be significant.Â Result: The changes in erythrocytes morphology stored at room temperature (18-25ÂºC) was that the erythrocytes crenation started to happen at 8 hours storage with the grading scale of +2 that were found in 24 (80%) samples, whereas at refrigerator temperature (2-8ÂºC) the grading scale was +1 and found in 13 (43.3%) samples. Spherocytes on erythrocytes began to form at room temperature (18-25ÂºC) at 8 hours storage with grading scale of +1 and was found in 2 (6.7%) samples, whereas at refrigerator temperatures (2-8ÂºC) spherocytes on erythrocytes began to form at 24 hours storage with grading scale of + 2 and was found in 3 (10%) samples.Conclusion: Peripheral blood smear (PBS) examination shall be done immediately to obtain significant results.
Erythrocyte alloantibodies in chronic kidney disease patients receiving packed red cell transfusions in Sanglah General Hospital, Denpasar
Authors: Krisnawati Ni Komang , Herawati Sianny , Mulyantari Ni Kadek
Published: April 2020

Introduction: In Chronic kidney disease (CKD) patients, as the consequences of the pathogenesis, anemia due to the failure of erythropoietin production requires Packed Red Cell (PRC) transfusion. The presence of alloantibodies in CKD patients who routinely require transfusion in Bali has never been evaluated. This study aims to determine the erythrocyte alloantibodies of CKD patients who received PRC transfusions in Sanglah Hospital.Methods: This study was a cross sectional descriptive study in adult CKD patients with history of PRC transfusion more than twice.Results: A total of 50 patients with Chronic Kidney Disease consisting of 31 men and 19 women, mostly 46-65 years old (60%) and the most blood group were blood group O (44%). There were 3 patients with positive antibody screening and identification results, 2 male patients (66.67%) and 1 female (33.33%). Two patients 46-65 years (66.67%) while 1 person 18-45 years (33.33%), with 2 person with blood group A (66.67%) and one person with blood group O (33.37% ). These three patients presented multiple alloantibodies in the form of anti-K, anti-Kpa, anti-LuaConclusion: From Â the CKD patients who received PRC transfusions in Sanglah Hospital, Â±6% formed alloantibodies. All of the alloantibodies formed were multiple alloantibodies, in the form of anti-K, anti-Kpa and Lua.
Relationship of Plasma Cystatin C level and renal dysfunction in patients with preeclampsia
Authors: Sitepu Makmur , Sitepu Azano , Marpaung Johny , Aldiansyah Dudy , Ardiansyah Edy , Edianto Deri , Sukatendel Khairani
Published: April 2020

Background: Renal dysfunction is an important aspect of the pathophysiology of preeclampsia besides vascular endothelial dysfunction and placental hypoperfusion. Cystatin C is one of the most important inhibitors of extracellular protease enzymes and can be reliable marker for the calculation of Glomerular Filtration Rates (GFR). This study aimed to determine the relationship between plasma levels of cystatin C and renal dysfunction in patients with preeclampsia.Method: This was an analytical cross sectional study conducted at Haji Adam Malik General Hospital Medan, Dr. Pirngadi Hospital Medan, several affiliated hospitals of the Faculty of Medicine University of North Sumatra and Prodia Clinical Laboratory Medan. A total of 26 preeclamptic patients were included in this study. Samples were divided into renal dysfunction and without renal dysfunction group.Results: Eleven subjects with renal dysfunctions were mostly aged 20 â€“ 35 years (73.3%), and the other eight subjects without renal dysfunctions were at the age of 20 â€“ 35 years (72.7%). Most of the subjects had multigravida pregnancies, five subjects (33.3%) with renal dysfunctions, and six subjects (54.5%) without renal dysfunctions. The mean level of cystatin C in renal dysfunction (1.52 Â± 0.37 mg/L) was significantly higher than without renal dysfunction (1.17 Â± 0.12 mg/L) (p < 0.05).Conclusion: The cystatin C level in preeclamptic patients with renal dysfunctions patient was higher than those who did not have renal dysfunction.
The characteristics of mandibular fractures among patients attending Plastic Surgery Unit in Sanglah General Hospital, Bali, Indonesia: A preliminary study
Authors: Suryantari Sang Ayu Arta , Hamid Agus Roy Rusly Hariantana , Sanjaya I Gusti Putu Hendra
Published: April 2020

Background: Trauma is the fourth leading cause of death in the world and occurs at all ages. The high incidence of traffic accidents is equivalent to the increasing incidence of maxillofacial trauma, especially mandibular fracture. This study aims to determine the characteristics by age, gender, mechanism of injury and anatomical site of mandibular fractures among patients attending the Plastic Surgery Unit of Sanglah General Hospital as a preliminary studyMethods: A descriptive cross-sectional study was enrolled among 45 patients attending plastic surgery who recorded in medical records from January 2014 until December 2016 as well as met the inclusion criteria. Data were analyzed using SPSS version 17 and presented in percentage regarding Age, mechanism of injury, gender, and fracture siteResults: There were 45 patients with 61 mandibular fracture sites. Most of the patients were male (84.4%). Based on age, the mandibular fracture is most common in the productive age (20-30 years old) at 22 cases (36.1%). Majority cause of fractures was road traffic accident (82.2%). Parasymphysis region was the most common site of fracture (39.3%).Conclusion: The characteristics of mandibular fracture patients describe trauma patterns in the community. This finding is similar with some readily available literature with RTAs was the most common etiology and parasymphysis was the most common fracture site.
The relationship of lactic acid, neutrophil lymphocyte ratio and monocyte lymphocyte ratio with the outcomes of coronary arterial disease patients following coronary artery bypass surgery
Authors: Citradinata Cynthia , Widyastiti Nyoman Suci
Published: April 2020

Background: Coronary artery bypass surgery (CABG) is a surgical procedure for coronary arterial disease (CAD). It has a risk of postoperative complication, extending from a long length of stay in the intensive care unit (ICU) to death. CABG needs a long duration of perioperative time, which decreases tissue oxygenation. It causes increased lactic acid production and initiates the secretion of inflammatory factors. Neutrophil/lymphocyte ratio (NLR) and monocyte/lymphocyte ratio (MLR) become inflammatory signs that are caused by the failure of heart microcirculation during the operative procedure. This study aimed to determine the relationship of lactic acid, NLR and MLR with the outcomes of CAD patients following CABG surgery.Methods: This was a prospective study including 42 CAD patients who had undergone CABG procedure at Dr. Kariadi Hospital Semarang between December 2017 and July 2018. Lactic acid data were taken using the spectrophotometer method. Â NLR and MLR were taken by the calculation index. The outcomes data were ICU length of stay (LoS), complications, and death, which were obtained from the medical record. The data were analyzed using bivariate analysis.Results: There was a significant correlation between lactic acid with ICU LoS (p = 0.001), complication (p = 0.01), and mortality (p = 0.044). There was a significant correlation between NLR with ICU LoS (p = 0.003), complication (p = 0.009), and mortality (p = 0.014). There was a significant correlation between MLR with ICU LoS (p = 0.02), complication (p = 0.027), and mortality (p = 0.017). Conclusion: There was a significant correlation between lactic acid, NLR dan MLR with the outcomes of CAD patients (ICU LoS, complication and mortality) that have undergone CABG procedure.
Correlation between verruca vulgaris and superoxide dismutase
Authors: Fachri Farica Amanda , Jusuf Nelva K , Putra Imam B
Published: April 2020

Background: Verruca vulgaris is a form of benign tumors that infected epidermal cells caused by Human papillomavirus (HPV). In several studies states that imbalances in oxidant and antioxidant systems play an important role in HPV infection. SOD is a potent protective enzyme that selectively cleanses radicals of antioxidants by catalyzing dismutation to hydrogen peroxide.Aim: The study aimed to determine the correlation between SOD level and verruca vulgaris.Methods: This was an analytic study with a cross sectional design involving 35 subjects with verruca vulgaris and 35 healthy controls. Diagnosis of verruca vulgaris was made based on history and clinical examination. The authors conducted blood sampling and measurement of plasma SOD level to the patients and controls.Results: The study found that the mean of SOD levels in verruca vulgaris patients were higher (49.27 Â± 100.47 U/L) than controls (35.12 Â± 50.44 U/L), but it didnâ€™t find a correlation between verruca vulgaris and SOD with p = 0.511. The mean level of â‰¥ 6 months were higher (59.69 Â± 140.85 U/L) than < 6 months (39.43 Â± 36.09 U/L) with p = 0.369. The mean multiple lesion levels were higher (62.12 Â± 140.76 U/L) than the SOD level of patients with a single lesion (37.14 Â± 34.89 U/L) with p = 0.405. The mean of SOD levels of patients who had previously experienced verruca vulgaris was higher (72.79 Â± 151.73 U/L) than those who had not it (31.63 Â± 18.26 U/L) with p = 0.961.Conclusion: We didnâ€™t found a correlation between verruca vulgaris and SOD.
Betel nut (Areca catechu L.) extract toxicity on the kidney and liver of white rats (Rattus norvegicus)
Authors: Arjana Anak Agung Gde , Sukada I Made , Suratma N. Adi
Published: April 2020

Background: Medication using herbal medicine is one of the alternatives chosen to reduce the adverse effects caused by synthetic drugs. Betel nut (Areca catechu L) usually used as an alternative drug such as anthelmintic and others.Aim: The study aims to determine the chronic toxicity effect of betel nut.Method: Five hundred grams of simplicial powder was macerated using 95% ethanol in a container, then sealed and let them for three days. They were protected from light while repeatedly stirred. Rat was given Betel nut extract for three weeks. The histopathological examination was observed under the microscope.Result: The mean GOT serum, GPT serum, BUN, and creatinine serum levels between control (no extract given) and each dose, i.e., 0.2 ccs, 0.4 ccs, and 0.8 ccs, showed insignificant results (p > 0.05). According to the histopathological observation, there was no difference between control and treatment.Conclusion: Various orally doses of betel nut extract did not cause toxicity in white rats (rattus novergicus).Â
Short insight of pediatric cerebral venous thrombosis and the safety of intra-arterial heparin flushing as a new therapeutic method
Authors: Pramono Ardianto , Soetikno Ristaniah , Kartamihardja Achmad Hussein Sundawa , Maskoen Tinni Trihartini , Putranto Terawan Agus , Setiawan Erwin
Published: April 2020

Introduction. The incidence rate of Cerebral Venous Thrombosis (CVT) in children has been increased quite significantly due to the availability of advanced imaging technology and the trends of increasing awareness about health-related problem. Systemic anticoagulant was mainstay treatment for CVT. The author proposed a method of heparin deliveries through existing Digital Subtraction Angiography (DSA) procedure called Intra-arterial Heparin Flushing (IAHF). The safety of this procedure in children was still few known and reported. The purpose of this study is to provide insights on CVT and the potential complication of IAHF as the primary therapeutic modality.Methods. This was a descriptive longitudinal study involving all patients with age 0-18 years diagnosed with Cerebral Venous Thrombosis whom admitted in RSPAD Gatot Soebroto from 2016 to 2018 through MRI examination and will undergo IAHF Procedure as the primary treatment. All subject had signed informed consent regarding the nature of the study. The descriptive data were presented in tables and narratives.Results. The study found that the highest age group found in this study was between 6-12 years old (46,6%) with male-predominant (70,6%). The origins of the patients were mostly from Indonesia (88%) and several from Vietnam (12%). The most common symptoms of CVT in this study was the speech/communication problem (12%). The most common complication after IAHF procedure was wound swelling at the puncture site (2,6%).Conclusion. CVT predominantly affect male aged 6-12 years old with speech problem was the most common symptoms. IAHF procedure shown minimal side effects in pediatric with CVT.
Development of rabies eradication package, a family-based health promotion media to prevent rabies in Buleleng, Bali, Indonesia
Authors: Setiawan Komang Hendra , Probandari Ari Natalia , Pamungkasari Eti Poncorini , Tamtono Didik Gunawan
Published: April 2020

Background : Rabies occurred in Bali since 2008, and in 2017 rabies cases were still found in Bali, including in Buleleng Regency. Prevention of rabies in Bali must involve the participation of the community especially family, which is in direct contact with dogs. This study aims to develop family-based health promotion media, which is later called as the rabies eradication package, to overcome rabies in Buleleng, Bali.Methods:Â  This study uses the research and development (R&D) method. which consists of 5 phases, namely analysis, design, development, implementation and evaluation. at the implementation and evaluation stage researchers used a quasi-experimental approach. The total number of samples used was 100 families taken from 2 villages. The variables measured were the level of knowledge and attitudes towards prevention of rabies. data analysis using the Mann-Whitney U test.Result : Â Â This study develop rabies eradication package as a family-based health promotion media. This package consists of a rabies eradication book, a dog vaccination card, a medical history cards and bite wound treatment tools. Of the 100 respondents in the study sample, 50 respondents who were included in the treatment group, which received a rabies eradication package for 1 month, experienced a significant increase in knowledge (p <0.001) and attitudes (p <0.001) in prevention of rabies compared to the control group.Conclusion : Rabies eradication package can be used as a family-based health promotion media to increase rabies prevention efforts because it can increase knowledge and attitudes in preventing rabies
Characterizing the SLC39A14 zinc transporter and collagen products of the chondrocytes from the human umbilical cord-derived mesenchymal stromal cell
Authors: Kumara Wati Ketut Dewi , Pratiwix Endah Dianty , Dirgantara Yanni , Sartika Cynthia Retna , Dhamayanti Meita , Setiabudiawa Budi , Parwati Ida
Published: April 2020

Background: The SLC39A14 zinc transporter showed a role in the growth of the mouse model. There was no report regarding SLC39A14 protein expression in human chondrocyte, which might prevent further identification of SLC39A14â€™s function in human growth. This study aims to describe the SLC39A14 protein expression in chondrocyte using human umbilical cord (UC)-derived mesenchymal stromal cell (MSC) isolation and differentiation. Methods: A cross sectional study was conducted at the Faculty of Medicine, Universitas Padjajaran Bandung, Indonesia, in 2019. The UC was derived after the respondents signed informed consents were documented. Specimens transferred, UC tissue digestion, cell isolation, cultures, and passages were done under the laboratory standard protocols. The immuno-phenotyping and differentiation toward chondrocyte procedure were done after third passage. The SLC39A14 expression of the chondrocytes was observed under immunofluorescence microscopy after staining using polyclonal anti SLC39A14 antibody. The collagen 2A and 10A production was measured using the medium supernatant and analyzed using GraphPad Prism 8 edition.Results: The adherent fibroblast-like cells appeared on day 5th, which continued to proliferate and showed MSC characteristic with positive markers for CD 73, CD90 and CD105, but absent for negative lineage markers. Upon differentiation, cells positive for Alcian blue staining denoted the chondrocyte which shown capacity for further proliferation on Methyl Tetra Toluene assay. Zinc transporter SLC39A14 was expressed on cell membrane. The Collagen 2A produced at a mean level 0.097 ng/ml (SE 0.011;95%CI 0.073-0.12) and Collagen 10A at mean level 31.078 ng/ml (SE 3.792;95%CI 22.870-39.287). Conclusion: Zinc transporter SLC39A14 was expressed in monolayer human chondrocyte from UC-derived MSC. The chondrocyte shown capacity for proliferation and collagen-producing enables future utility to identify the role of SLC39A14 in chondrocyte pathology.
The effect of tomato juice on the expression of matrix metalloproteinase-2 (MMP-2) and type-1 collagen on the vaginal wall of the menopausal rats
Authors: Saimin Juminten , Hendarto Hendy , Soetjipto Soetjipto
Published: April 2020

Background: Aging is a multifactorial phenomenon that is characterized by a decrease in physiological functions that increases with age. Free radicals cause oxidative stress, which plays a role in the aging process. The accumulation of reactive oxygen compounds increases matrix metalloproteinase-2. Collagen can be directly destroyed by reactive oxygen compounds or through the induction of MPPs. Tomato fruit is an antioxidant, which acts as a binder and damper of free radicals that activate collagen damage. This study aims to assess the effect of tomato juice on the expression of MMP-2 and type-1 collagen on the vaginal wall of the menopausal rat.Methods: This was a true experimental study, using Sprague-Dawley rats and tomato juice (Solanum lycopersicum). Expression of MMP-2 and type-1 collagen was examined using immunohistochemistry staining. Twenty-four healthy female rats, aged 4 months weighing 150-200 grams, were divided into 4 groups, each group as many as 6 rats. The negative control group (NC) was a group of rats with a sham procedure, given aquades for 28 days. The positive control group (PC) was bilaterally ovariectomized rats, given aquades for 28 days. The first treatment group (P1) was bilaterally ovariectomized rats, given tomato juice with a dose of 11 g / 200 g BW / day. The second treatment group (P2) was bilaterally ovariectomized rats, given tomato juice with a dose of 15 g / 200 g BW / day. Data analysis used the One-way ANOVA statistical test (Î± = 0.05) and different tests between groups using Games-Howell and Tukey HSD. The association between tomato juice and the expression of MMP-2 and type-1 collagen used regression tests.Results: This study found that the lowest expression of MMP-2 was NC group (2.07 Â± 0.84), and the highest was the PC group (5.72 Â± 1.91). The mean MMP-2 expression in the treatment groups was lower than the PC group but still higher than the NC group. There was a significant difference in MMP-2 expression between groups (p = 0,000). There was a negative correlation between tomato juice administration and MMP-2 expression (b = -0.592). The lowest type-1 collagen expression was PC group (3.83 Â± 1.44), and the highest was the P2 group (7.27 Â± 2.21). The mean type-1 collagen expression in the treatment groups was higher than the PC group. There were significant differences of type-1 collagen expression between groups (p = 0.012). There was a positive correlation between tomato juice administration and type-1 collagen expression (b = 0.682).Conclusions: Tomato juice administration can reduce the expression of MMP-2 and increase the expression of type-1 collagen on the vaginal wall of the menopausal rat. The higher the dose of tomato juice, the higher the expression of type-1 collagen.
Red guava juice (Psidium guajava linn.) suppress TGF-Î² protein expression in secondary hyperuricemia mice (Mus musculus) model
Authors: Ayu Riska Nur Suci , Wasita Brian , Dirgahayu Paramasari
Published: April 2020

Background: Secondary hyperuricemia can increase the risk of renal dysfunction in the form of fibrosis and glomerulosclerosis mediated by TGF-Î² signalling. Non-pharmacological therapy through high dietary antioxidant intakes can help in controlling and preventing renal dysfunction by suppressing TGF-Î² expression. This study aimed to analyse the effect of red guava juice (Psidium Guava Linn.) on TGF-Î² protein expression in mice (Mus Musculus) kidneys with secondary hyperuricemia model. Method: This was an experimental study with a post-test only design. Forty-eight mice (Mus Musculus) were choosen as samples, which were distributed into six different treatment groups. The mice were induced with potassium oxonate for 14 days and given red guava juice for 21 days. TGF-Î² protein expression in mice (Mus Musculus) kidneys was identified through immunohistochemistry using TGF-Î² antibodies. The preparation was observed in the Anatomical Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret using the Olympus XC10 light microscope. Data were statistically analysed using the Kruskal Wallis test, followed by the Post-Hoc Mann Whitney test. Results: The results of this study showed that the administration of red guava juice had a significant effect on TGF-Î² expression (p = 0.001). There was a significant difference between the negative control treatment and treatment of red guava juice at a dosage of 5 ml/kg/day (p = 0.043).Conclusion: It can be concluded that the administration of red guava juice in mice (Mus Musculus) with secondary hyperuricemia model can suppress TGF-Î² expression, and therefore, can be used as non-pharmacological therapy.
EGFR nanovaccine in lung cancer treatment
Authors: Anggaraditya Putu Bagus , Adiputra Putu Anda Tusta , Widiana I Ketut
Published: April 2020

Lung cancer is known as the most common malignancy in the world in terms of incidence and death rate. GLOBOCAN data in 2018 showed that its prevalence reaches 11.6% with mortality reaching 1.7 million annually. The prevalence of lung cancer in Indonesia is also considerably high; there were 25,332 cases in men and 9,374 cases in women with mortality reaching 308,660 people. In general lung cancer is classified as non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Although it is less common, SCLC has a worse prognosis with a 5 year survival rate of 6.4%. In addition, SCLC is also often diagnosed when it has metastasized or already reaced extensive stage (ES-SCLC). Although SCLC is classified as chemo-responsive cancer, the overall outcome of first-line and second-line therapy is still unsatisfactory with an ORR of 10-25%. One of characteristic of SCLC is genomic instability which relates to high level of mutations especially EGFR mutation that strongly correlate with therapeutic outcome. Therefore, targeting EGFR mutation is a sensible and potential field in developing SCLC therapy. EGFR-CpG-ODN nanovaccine is one of the potential therapeutic choices that exploit this mutation. EGFR CpG ODN nanovaccine could inhibit resistance to EGFR TKI, increased dendritic cell maturation, inhibit cancer cell proliferation and apoptosis, as well as enhance the anticancer immune response. Therefore, this therapeutic approach is a promising future therapy for SCLC petients that could improve patientâ€™s survivability.
The potential application of conditioned media-mesenchymal stem cells on human oocyte maturation in assisted reproductive technology: a quasi-experimental based-study at Dr. Sardjito General Hospital, Yogyakarta, Indonesia
Authors: Haryadi Dwi , Sadewa Ahmad Hamim , Mubarika Sofia , Dasuki Djaswadi
Published: April 2020

Background: PCOS typically characterized by a decreased level of GDF9 and BMP15, which contribute to follicular arrest. Recent research related to stem cells showed that conditioned media-mesenchymal stem cell (CM-MSC) might have a positive role in folliculogenesis. Therefore, this study aimed to assess the effectiveness of CM-MSC towards oocyte maturityMethod: A quasi-experimental design was used to examine the outcome of 3 different interventions, namely group A (4-hour incubation in standard media), group B (24-hour incubation in standard media), and group C (24 hours incubation in conventional media with addition of CM-MSC). Nuclear and cytoplasmic maturity, along with GDF9 and BMP15 levels were measured and analyzed.Results: Sixty-three patients at an infertility clinic, RSUP Dr. Sardjito, Yogyakarta was recruited and a total of 292 germinal vesicles were obtained to start in vitro maturation procedure. Multivariate analysis showed group C has OR 6.9 (3,3â€“14,41) to obtain metaphase II oocyte than group B (p <0,0001). Infertility causes, insulin resistance, and maternal age risk are other factors that significantly influence oocyte maturity outcome (p <0.001; p = 0.005; p = 0.017). For the oocyte morphology outcome, no significant effect was obtained from the intervention (p> 0.05). Group C has higher GDF9 levels (Î” mean = 3.31) and BMP15 (Î” mean = 1.52) compared with group B (p <0.001; p = 0.006).Conclusion: It can be concluded that 24-hour incubation in CM-MSC was effective to induce oocyte maturation. However, other factors, such as infertility causes, insulin resistance, and maternal age, should also be considered.
Mitogen-activated protein kinase 3 (MAPK3) and human epidermal growth factor receptor 2 (HER2) on recurrent intracranial meningiomas: a case report
Authors: Dharmajaya Ridha , Mouza Abdurrahman
Published: April 2020

Introduction. Meningiomas accounts for one-third of all adult central nervous system tumors and by far the most common primary intracranial tumor in adults. Despite their high prevalence, it remains challenging to predict their biological and clinical behavior. To date, it has been accepted that the extent of surgical resection and histological grade are the most reliable factors to predict the recurrence of meningiomas. HER2 and MAPK3 represent a well-known prognostic factor in various tumors, however only a few studies on the relationship between meningioma recurrence, HER2, and MAPK3 expression. This report presents additional data that support the relationship between the expression HER2 and MAPK3 with recurrent of meningioma.Case: We report 39 years-old-female with recurrent meningioma. The patient had tumor removal a year ago. After the first resection, the tumor demonstrated rapid growth with significant invasion to neighboring dural tissue and brain parenchyma, which resulted in impaired brain function and intracranial hypertension manifested as severe headache, vomiting, and coma. Histopathological examination confirms a malignant meningioma (WHO grade I). The patient had the second craniotomy for tumor removal, and we performed immunohistochemical staining. The result was positive for HER2 and MAPK3Conclusion: HER2 and MAPK3 might be a reliable predictor of recurrent meningiomas.Â
Association of serum interleukin-10 (IL-10) with the severity of acne vulgaris
Authors: Rahmayani Tissan , Putra Imam Budi , Jusuf Nelva K
Published: April 2020

Background: Acne vulgaris is a common inflammatory disorder in pilosebaceous gland units, which affected more than 90% of teenagers, and half of them settled until adulthood. Inflammation is one of the four critical factors in the pathogenesis of acne vulgaris, and it could be a primary process or secondary to Propionibacterium acnes. The immune system has a various anti-inflammatory mechanism to counter-balance inflammatory mediators. One of the potent cytokines that play a role in regulating the inflammatory state is Interleukin-10 (IL-10). This study conducted to determine the association of serum IL-10 with the severity of acne vulgaris.Methods: This is a cross-sectional analytic study involving 33 subjects with acne vulgaris and 31 normal-healthy volunteers as a control. Both groups had their serum IL-10 measured. Acne vulgaris severity was assessed with Lehmann criteria. Anova was used to determine the statistical difference of IL-10 among different groups of acne severity. This study has been approved by the Ethical Committee Faculty of Medicine, Universitas Sumatera Utara.Results: The mean serum IL-10 of normal-healthy and acne vulgaris subjects were 5,79 Â± 1,23 pg/ml and 5,25 Â± 1,55 pg/ml, respectively. Mann-Whitney test showed a significant difference (p=0,029). The mean IL-10 serum levels according to acne vulgaris severity were 6,63 Â± 1,94 pg/ml in the mild category; 4,89 Â± 0,95 pg/ml in the moderate category and 4,38 Â± 0,61 pg/ml in the severe group. ANOVA showed significant difference with p=0,001.Conclusion: There was an association between serum levels of IL-10 with the severity of acne vulgaris.
Developing Indonesian version of childbirth self-efficacy inventory (Cbsei)-C32
Authors: Kartini Farida , Emilia Ova , Dasuki Djaswadi , Prabandari Yayi Suryo
Published: April 2020

Introduction: Women readiness and self-efficacy for pregnancy and childbirth is an important issue which usually assessed by using CBSEI-32 but currently no evaluation is ever conducted nor Indonesian version of this instrument ever developed to enhance subject understanding. Therefore, this study aimed to develop the Indonesian Language version of CBSEI (childbirth self-efficacy inventory) instrument.Method: A quantitative study was conducted by involving 73 pregnant mothers in their trimester III either as the primigravida or as multigravida. The selected respondents are those who lived in the working area or visited several health care centres in the area between July-August 2018. The content validity was tested by using Aiken V analysis and the construct validity was assessed using Kaiser-Meyer-Olkin (KMO) and Bartlettâ€™s test. Finally, the reliability test was conducted using Alpha Cronbach.Result: Â Analysis revealed that the Aiken V value of the instrument was in the range of 0.67-1 for the outcome expectancy and 0.75-1 for efficacy expectancy. 16 items in outcome expectancy showed KMO value > 0.5 with MSA value between 0.648-0.957.Â  Meanwhile, 16 questions in efficacy expectancy also had KMO value > 0.5 with a MSA range between 0.798-0.937 respectively. The average values of the instrument validity for the outcome expectancy and efficacy expectancy were 0.740 and 0.726 respectively. Finally, Alpha Cronbach value for both outcome expectancy and efficacy expectancy was 0.94.Conclusion: According to the analysis, it can be concluded that CBSEI-C32 in Indonesia language version was valid and reliable instrument to be applied in pregnant mother in Indonesia.Â
Identification factors affecting adolescentâ€™s reproductive health behavior: a qualitative study
Authors: Triyanto Endang , Prabandari Yayi Suryo , Yuniarti Kwartarini Wahyu , Werdati Sri
Published: April 2020

Introduction: Sexual risk behavior is mostly conducted by adolescents when the puberty started.Â The motivations underlies such behavior are varied including those related to individual, peers, family, and the environment. Therefore, this study aimed toÂ exploreÂ the factors that affect adolescentâ€™s reproductive health behavior.Methods: This study used qualitative method with purposive sampling on high risk and low risk adolescents.Â ExplorationÂ was conducted in accordance with several behavior theories such as Ecological Model of Health Behavior, Problem Behavior TheoryÂ and Integrated Behavior Model.Â Data collection was conducted by in-depth interviewsÂ in 2018 at Banyumas District.Â The results of the interviews wereÂ then analyzed by content analysisÂ technique.Â Results: According to the results ofÂ content analysis, weÂ found that reproductive behavior among adolescents was affected by two broad categories of factors namely individual internal factors and external factors.Â Individual internal factors were including age related risk, self-helplessness, knowledge, attitude, perceived norm, and lifestyle.Â External factors were determined by negative influence of peers, family, and environment risk. Peers, family, lifestyle and external factors seemed to influence personal values which could act as risk factor or protective factor depending on the nature of those factors involved. Conclusion: This research revealed that reproductive health behavior among adolescents was influenced by multiple factors that interact with one another.Â
The relationship of hormonal receptor, HER-2, and KI-67 changes after administration of anthracycline-based neoadjuvant chemotherapy with the results of histopathological grading in stage III breast cancer patients at Saiful Anwar Malang Regional Public H
Authors: Wintoko Risal , Susilo Hery
Published: April 2020

Background: Breast cancer is cancer, with the highest incidence in women worldwide. It also has a very high mortality due to late treatment. The immunohistochemical examination is an important factor in determining breast cancer subtypes and subsequent therapy. However, neoadjuvant chemotherapy (NACT) has been reported to change the expression of immunohistochemical examinations of Estrogen Receptor (ER), Progesterone Receptor (PR), Human Epidermal Growth Factor Receptor-2 (HER-2), and Ki-67. This study aims to determine the relationship between hormonal, HER-2, and KI-67 changes after NCAT.Method: The immunohistochemical examinations were conducted on ER, PR, HER-2, Ki-67 and histopathological grading from 59 cases of stage III breast cancer after NACT administration. We analyzed the relationship between the changes in the expression of immunohistochemical examinations on the biopsy tissue specimens before NACT administration and from the tissue mastectomy after NACT administration associated with its histopathological grading with spearman correlation analysis using SPSS version 25 for Windows.Results: There were changes in the results of the expression of immunohistochemical examinations of ER, PR, HER-2, KI-67 and histopathological grading by 23.7%, 22.03%, 32.2%, 32.2% and 40.68% in stage III breast cancer patients who received NACT. There was a relationship with a weak correlation between the changes in immunohistochemical examinations of ER, PR, HER-2 and KI-67 with the changes in histopathological grading. The correlation coefficients between ER, PR, HER-2, Ki-67 and the histopathological grading were r=0.265, r=0.317, r=0.352 and r=0.335.Conclusions: Patients with stage III breast cancer who were treated with NACT experienced changes in the expression of immunohistochemical examinations of ER, PR, HER-2 and KI-67 and would experience changes in histopathological grading, causing changes in the breast cancer subtypes.
Correlation between Loss of Cervical Lordosis and Degenerative Diseases of the Sub-axial Cervical Spine Columns
Authors: Martadiani Elysanti Dwi , Asih Made Widhi , Laksminigsih Nyoman Srie , Tjan Anastasia , Widyasari Nyoman
Published: April 2020

Background: Subaxial cervical spine is the cervical segment which commonly undergo degenerative changes. There are numerous cervical lordosis angle measurement, however posterior tangential method is considered more accurate to detect any degenerative related changes.Aim: This study aimed to find the profile of cervical lordotic curve among patients with cervical degenerative disease, and to determine the correlation between degenerative process of subaxial cervical spine columns and cervical curve malalignment.Method: A cross sectional study was conducted by adopting the posterior tangent measurement. Cervical alignment was measured from cervical MRI mid sagittal section to identify loss of cervical lordotic and the presence of kyphosis. Degenerative disease of each vertebral column of subaxial cervical spine, anterior and posterior were evaluated. Spearman correlation test was done to identify the relationship between degenerative process of each column of subaxial cervical spine with loss of cervical lordosis and the presence of kyphosis. Multivariate analysis was done to adjust the effect of confounding variable.Result: Total of 90 subjects with male predominance (62%), mean age 54Â± 1.3 years. Neck pain contributes the most presenting complain (92%). As many as 51 subjects have hypo-lordosis, while 32 subjects have kyphotic changes. There was no significant correlation between degenerative process of each column of subaxial cervical spine and loss of cervical lordotic, but significant correlation was found between posterior listhesis and cervical kyphosis defomity. (p < 0.05).Conclusion: There was significant correlation between cervical kyphotic malalignment with posterior listheis Â in all three vertebra columns instead of hypo-lordosis.
The effect of bioabsorbable (fibrillar) and fibrin glue on the number of macrophages and fibroblasts in Wistar rats with gastric perforation compared to the omental patch method
Authors: Resdiyanto Resdiyanto , Niam Muhammad Shobachun , Rasyid Harun Al , Sugiharto Setyo , Inggarwati Lulik , Susilo Hery
Published: April 2020

Â Background: Gastric perforation covers 25-30% of acute abdomen in the ER and has high mortality and morbidity rate. Surgery is the primary treatment of gastric perforation. The omental patch is commonly used to close the gastric perforation. One method is to use bioabsorbable material glued with fibrin glue. This study aims to determine the effect of bioabsorbable material as a sutureless technique in wound healing of gastric perforation.Method: A true laboratory experimental post-test only control group design using a single-blind technique was conducted among 20 Wistar rats. They were divided into 2 random groups, 10 fibrillar and fibrin glue group and 10 omental patch group. Perforation was made by forming a small hole with a diameter of 0.5 cm using a punch biopsy. The microscopic examination with Hematoxylin-eosin staining was carried out for the number of macrophages and fibroblasts from the gastric perforation site. Data were analyzed using SPSS version 16 for Windows.Result: The average number of fibroblasts produced by the fibrillar and fibrin glue group was 7.5 and 5.4 in omental patch group. Based on the result of Â T-test (fibroblasts), it was obtained that there was a significant difference (H1 is accepted) in the number of fibroblasts produced using fibrillar and fibrin glue compared to the omental patch method (P<0.001). The average number of macrophages in fibrillar and fibrin glue group was 4 and 3 in omental patch group. Based on T-test, it was found a significant difference in the number of macrophages produced using fibrillar and fibrin glue compared to the omental patch method (P<0.05)Conclusion: The administration of fibrillar and fibrin glue biomaterials in the closure of gastric perforation increased the number of macrophages and fibroblast cells better than the omental patch method.
Reproductive health promotion through traditional art media reduced the of premarital sex intention among adolescent population in Gianyar, Bali
Authors: Widarini Ni Putu , Tetradewi Fatwa Sari , Emilia Ova
Published: April 2020

Background: Sex education in adolescents is still considered taboo to be discussed in the community. Traditional arts as communicative and informative socialization media contain meaningful messages, it is expected that teenagers get a good understanding of reproductive health so that they become healthy and quality adolescents. Therefore, this study aimed to evaluate reproductive health promotion through traditional arts media on adolescent knowledge, attitudes and sexual intentions in Gianyar, Bali.Methods: A quantitative study with a quasi-experimental design was conducted with nonequivalent (pretest and posttest) control group. Data on premarital sex knowledge, attitudes and intentions were collected before and after staging traditional arts in the intervention group (75 adolescents). Data were collected from self-filled questionnaires and analyzed using STATA (version 12.0). Data analysis was conducted using Wilcoxon rank test and Wilcoxon rank-sum.Results: The results showed an overall significant improvement in the knowledge and attitudes of adolescents about premarital sex and a decrease intention toward premarital sex after intervention (p value 0.00). The improvement in knowledge, premarital sex attitudes and intentions was more pronounced in the intervention group compared to control group. The control group that was not given an intervention showed a higher average value of premarital sex intention. Conclusion: Reproductive health promotion program using traditional arts media was able to significantly reduce premarital sex intentions among teenagers.Â Â Â Â Â