bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of the Kinetic Parameters of Alternative Oxidases From Trypanosoma brucei and Arabidopsis thaliana—A Tale of Two Cavities
Authors: Xu Fei , Copsey Alice C. , Young Luke , Barsottini Mario R. O. , Albury Mary S. , Moore Anthony L.
Published: October 2021

The alternative oxidase (AOX) is widespread in plants, fungi, and some protozoa. While the general structure of the AOX remains consistent, its overall activity, sources of kinetic activation and their sensitivity to inhibitors varies between species. In this study, the recombinant Trypanosoma brucei AOX (rTAO) and Arabidopsis thaliana AOX1A (rAtAOX1A) were expressed in the Escherichia coli ΔhemA mutant FN102, and the kinetic parameters of purified AOXs were compared. Results showed that rTAO possessed the highest Vmax and Km for quinol-1, while much lower Vmax and Km were observed in the rAtAOX1A. The catalytic efficiency (kcat/Km) of rTAO was higher than that of rAtAOX1A. The rTAO also displayed a higher oxygen affinity compared to rAtAOX1A. It should be noted that rAtAOX1a was sensitive to α-keto acids while rTAO was not. Nevertheless, only pyruvate and glyoxylate can fully activate Arabidopsis AOX. In addition, rTAO and rAtAOX1A showed different sensitivity to AOX inhibitors, with ascofuranone (AF) being the best inhibitor against rTAO, while colletochlorin B (CB) appeared to be the most effective inhibitor against rAtAOX1A. Octylgallate (OG) and salicylhydroxamic acid (SHAM) are less effective than the other inhibitors against protist and plant AOX. A Caver analysis indicated that the rTAO and rAtAOX1A differ with respect to the mixture of polar residues lining the hydrophobic cavity, which may account for the observed difference in kinetic and inhibitor sensitivities. The data obtained in this study are not only beneficial for our understanding of the variation in the kinetics of AOX within protozoa and plants but also contribute to the guidance for the future development of phytopathogenic fungicides.
Constraints and Prospects of Improving Cowpea Productivity to Ensure Food, Nutritional Security and Environmental Sustainability
Authors: Omomowo Olawale Israel , Babalola Olubukola Oluranti
Published: October 2021

Providing safe and secure food for an increasing number of people globally is challenging. Coping with such a human population by merely applying the conventional agricultural production system has not proved to be agro-ecologically friendly; nor is it sustainable. Cowpea (Vigna unguiculata (L) Walp) is a multi-purpose legume. It consists of high-quality protein for human consumption, and it is rich in protein for livestock fodder. It enriches the soil in that it recycles nutrients through the fixation of nitrogen in association with nodulating bacteria. However, the productivity of this multi-functional, indigenous legume that is of great value to African smallholder farmers and the rural populace, and also to urban consumers and entrepreneurs, is limited. Because cowpea is of strategic importance in Africa, there is a need to improve on its productivity. Such endeavors in Africa are wrought with challenges that include drought, salinity, the excessive demand among farmers for synthetic chemicals, the repercussions of climate change, declining soil nutrients, microbial infestations, pest issues, and so forth. Nevertheless, giant strides have already been made and there have already been improvements in adopting sustainable and smart biotechnological approaches that are favorably influencing the production costs of cowpea and its availability. As such, the prospects for a leap in cowpea productivity in Africa and in the enhancement of its genetic gain are good. Potential and viable means for overcoming some of the above-mentioned production constraints would be to focus on the key cowpea producer nations in Africa and to encourage them to embrace biotechnological techniques in an integrated approach to enhance for sustainable productivity. This review highlights the spectrum of constraints that limit the cowpea yield, but looks ahead of the constraints and seeks a way forward to improve cowpea productivity in Africa. More importantly, this review investigates applications and insights concerning mechanisms of action for implementing eco-friendly biotechnological techniques, such as the deployment of bio inoculants, applying climate-smart agricultural (CSA) practices, agricultural conservation techniques, and multi-omics smart technology in the spheres of genomics, transcriptomics, proteomics, and metabolomics, for improving cowpea yields and productivity to achieve sustainable agro-ecosystems, and ensuring their stability.
Flourishing Through Music Creation: A Qualitative Investigation of the Lullaby Project Among Refugee and Incarcerated Communities
Authors: Ascenso Sara
Published: October 2021

The Lullaby Project is an innovative model developed to support vulnerable groups through community-based music creation. It pairs expectant and new mothers with professional musicians, to create a lullaby for their children. This paper presents an investigation of the project’s pilot implementation in the United Kingdom, bringing together musicians from the Royal Philharmonic Orchestra, refugee mothers and inmate fathers from a central London prison. The research aimed to understand how the Lullaby Project was experienced, focusing on the potential areas of perceived change linked with the concept of mental health as flourishing. Participants (N=12) took part in semi-structured interviews and kept daily notes to aid recollection of the sessions in the interviews. Interpretative phenomenological analysis was adopted as the research approach. Participants considered the project to carry significance for them in three key areas: (1) wellbeing, through a strong sense of accomplishment, meaning and connectedness, and the experience of positive emotions; (2) proactivity, promoting initiative, both musical and relational; and (3) reflectiveness, stimulating perspective-taking and positive coping mechanisms. The Lullaby Project offers an effective model towards promotion of flourishing among vulnerable groups, and the results make a strong case for its implementation.
A Contactless Method for Measuring Full-Day, Naturalistic Motor Behavior Using Wearable Inertial Sensors
Authors: Franchak John M. , Scott Vanessa , Luo Chuan
Published: October 2021

How can researchers best measure infants' motor experiences in the home? Body position—whether infants are held, supine, prone, sitting, or upright—is an important developmental experience. However, the standard way of measuring infant body position, video recording by an experimenter in the home, can only capture short instances, may bias measurements, and conflicts with physical distancing guidelines resulting from the COVID-19 pandemic. Here, we introduce and validate an alternative method that uses machine learning algorithms to classify infants' body position from a set of wearable inertial sensors. A laboratory study of 15 infants demonstrated that the method was sufficiently accurate to measure individual differences in the time that infants spent in each body position. Two case studies showed the feasibility of applying this method to testing infants in the home using a contactless equipment drop-off procedure.
Music Does Not Facilitate Lexical Tone Normalization: A Speech-Specific Perceptual Process
Authors: Tao Ran , Zhang Kaile , Peng Gang
Published: October 2021

Listeners utilize the immediate contexts to efficiently normalize variable vocal streams into standard phonology units. However, researchers debated whether non-speech contexts can also serve as valid clues for speech normalization. Supporters of the two sides proposed a general-auditory hypothesis and a speech-specific hypothesis to explain the underlying mechanisms. A possible confounding factor of this inconsistency is the listeners’ perceptual familiarity of the contexts, as the non-speech contexts were perceptually unfamiliar to listeners. In this study, we examined this confounding factor by recruiting a group of native Cantonese speakers with sufficient musical training experience and a control group with minimal musical training. Participants performed lexical tone judgment tasks in three contextual conditions, i.e., speech, non-speech, and music context conditions. Both groups were familiar with the speech context and not familiar with the non-speech context. The musician group was more familiar with the music context than the non-musician group. The results evidenced the lexical tone normalization process in speech context but not non-speech nor music contexts. More importantly, musicians did not outperform non-musicians on any contextual conditions even if the musicians were experienced at pitch perception, indicating that there is no noticeable transfer in pitch perception from the music domain to the linguistic domain for tonal language speakers. The findings showed that even high familiarity with a non-linguistic context cannot elicit an effective lexical tone normalization process, supporting the speech-specific basis of the perceptual normalization process.
The Influence Mechanism of Knowledge-Based Professionals’ Core Value Identity on Creativity From the Perspective of the Knowledge Economy
Authors: Zhou Yilin , Wang Chunyan , Shahzad Fakhar , Murad Majid
Published: October 2021

In the era of the knowledge economy, tacit knowledge transfer is a key strategy for developing personal creativity, but it also is affected by core value agreement. Knowledge-based professionals have innate sensitivity with core value agreement. In the knowledge capital development system, tacit knowledge transfer is a key strategy to be discovered. This research investigates the core value identity mechanism of knowledge professionals from the perspective of the knowledge economy. The results revealed the intrinsic relationship between core values identity, tacit knowledge transferring, and personal creativity based on knowledge-based professionals’ data and employing core values identity, tacit knowledge transferring, and personal creativity based on knowledge-based professionals’ data and structural equation modeling. The results also represent the incentive path of tacit knowledge transferring in personal creativity under the core value agreement condition, which may provide a theoretical inspiration to foster knowledge-based talent creativity.
Deep Learning-Based Teaching Strategies of Ideological and Political Courses Under the Background of Educational Psychology
Authors: He Xiaoqing , Chen Peiyao , Wu Jieting , Dong Zhen
Published: October 2021

At present, low teaching efficiency has been the common problem of ideological and political education in colleges and universities in China. It is essential to improve the teaching efficiency and realize the intelligent information transformation of the ideological and political courses in colleges and universities. First, the relationship between ideological and political courses and the educational psychology of college students was analyzed based on the theoretical characteristics of educational psychology and college ideological and political courses. Additionally, the teaching efficiency of ideological and political courses based on deep learning (DL) was analyzed through a literature survey. Combined with online teaching modes such as the flipped classroom and Massive Open Online Courses, a comprehensive online teaching mode of college ideological and political courses was proposed via educational psychology and the Single Shot MutiBox Detector networks of DL. Then, a total of 100 research subjects were selected randomly from the freshmen and sophomores of the Southwest University of Science and Technology, and their acceptability to the online ideological and political courses was analyzed by a questionnaire survey. The results show that the adopted questionnaire had high reliability and validity, and the proportion of respondents of different genders, grades, and majors was essentially balanced. More than half of the students had a good understanding of the comprehensive ideological and political courses and made progress in their values, ideology, morals, and knowledge reserves. More than half of the students had a positive attitude to the course, and they thought that the class atmosphere of the course was active, which was conducive to a satisfactory learning effect. This indicates that the teaching strategy of ideological and political courses in colleges and universities that integrates educational psychology, DL, and online information can attract students. The contribution of this study is that the research outcome can be applied to the concrete formulation of the teaching strategies of ideological and political courses for college students.
On the Psychology of Argument: A Structural Analysis of Former Muslims' Postings Within Malaysian Social Media
Authors: Hashmi Umair Munir , Rashid Radzuwan Ab , Munir Rabia
Published: October 2021

This research attempts to provide insights into the argumentation structures in the discussion of Islam on social media involving 14 Malaysian former Muslims. The social media accounts of the participants were observed for 12 months, from January to December 2019. A total of 368 postings put forth arguments related to Islamic authoritative discourse, the Quran and “Sunnah” of the Prophet Muhammad, to justify their renunciation of the Muslim religion. The analysis revealed that the Level 2 argument, which includes the claim, data to support the claim, and the warrant, was identified as the most common argument structure. Level 5, which has more than one rebuttal, was the least common argument structure. The analysis shows that most argument structures were at the lower levels (1–3) in that they offered no strong, clearly identifiable rebuttals. This study concludes that the arguments put forth by former Muslims, in the main, are loosely constructed rather than attempts to build a strong cumulative argumentation to support their reasons for abandoning the Muslim faith.
Paradoxical Effects of Performance Pressure on Employees’ In-Role Behaviors: An Approach/Avoidance Model
Authors: Xu Xiaofeng , Wang Yihui , Li Miaomiao , Kwan Ho Kwong
Published: October 2021

Performance pressure acts as a double-edged sword for employees. Based on an approach/avoidance framework, we theorize that performance pressure produces both positive and negative effects on employees’ in-role behaviors via approach motivation (i.e., self-objectification) and avoidance motivation (i.e., workplace anxiety), and work meaningfulness moderates employees’ reactions to performance pressure. We examine our hypotheses using data from a sample of 345 employees in various organizations. The results show that self-objectification provides an approach motive that mediates the positive indirect effect of performance pressure on employees’ in-role behaviors. However, workplace anxiety provides an avoidance motive that mediates the negative indirect effect of performance pressure on employees’ in-role behaviors. Work meaningfulness strengthens both the approach and avoidance tendencies that employees experience under performance pressure. Our findings have significant theoretical and managerial implications.
Traumatic Events, Social Adversity and Discrimination as Risk Factors for Psychosis - An Umbrella Review
Authors: Varchmin Leonie , Montag Christiane , Treusch Yvonne , Kaminski Jakob , Heinz Andreas
Published: October 2021

Exposure to childhood trauma is a well-known risk factor for severe mental disorders including schizophrenia and other non-affective psychoses. Beyond childhood trauma, there is increasing evidence that bullying, social exclusion, and discrimination during adolescence and adulthood may increase the risk of developing a psychotic disorder, and that such forms of traumatization may also underlie the elevated psychosis risk among migrants or persons with a visible minority status. In this umbrella review, we systematically assess meta-analyses regarding trauma and social adversity. A systematic literature review yielded 11 meta-analyses that met inclusion criteria and could be summarized quantitatively with a random effect model. Furthermore, six meta-analyses were evaluated qualitatively. Heterogeneity and publication bias were apparent in several meta-analyses. We observed that most significant social risk factors for psychosis were vulnerability for racist discrimination [OR = 3.90 (3.25–4.70)], migration [OR = 2.22 (1.75–2.80)], and childhood adversities [OR = 2.81 (2.03–3.83)]. Furthermore, social factors increasing the risk for psychosis were variation/impairment of parental communication, aversive adult life events, bullying, and factors associated with social isolation and discrimination. In spite of these environmental risk factors, there is a lack of evidence regarding treatment of trauma and psychosis, although some psychotherapeutic and art therapy approaches appear to be promising. Beyond individual interventions, stigmatization, racism, and other forms of discrimination need to be targeted to increase solidarity and communal support.
Childhood Maltreatment Predicts Specific Types of Dysfunctional Attitudes in Participants With and Without Depression
Authors: Jugessur Raj , Zhang Yan , Qin Xuemei , Wang Mi , Lu Xiaowen , Sun Jinrong , Dong Qiangli , Zhang Liang , Liu Jin , Ju Yumeng , Liao Mei , Wan Ping , Guo Hua , Zhao Futao , Liu Bangshan , Li Lingjiang
Published: October 2021

Background: Studies have shown a strong association between childhood maltreatment (CM) and major depressive disorder (MDD). Dysfunctional attitudes (DAs) play a crucial role in the development of MDD. In this study, we aimed to investigate whether (1) DAs are associated with CM, (2) specific CM types predict specific types of DAs, and (3) higher childhood trauma counts (CTCs) predict more DAs.

Methods: One hundred seventy-one MDD participants and 156 healthy controls (HCs) were enrolled for the study. CM was assessed retrospectively with the Childhood Trauma Questionnaire. DAs were evaluated using the Chinese version of the Dysfunctional Attitude Scale–Form A (C-DAS-A). A series of analyses, including multiple analyses of covariance and hierarchical regression analyses, were used in this study to examine the hypotheses.

Results: The proportion of CM was 60.2% in the MDD group and 44.2% in the HC group. The 2 × 2 analysis of covariance results showed no interaction effect between CM and MDD on C-DAS-A total score. When the factor scores replaced the C-DAS-A total score, a similar trend was observed. Within the MDD group, emotional abuse (EA) predicted two forms of DAs: self-determination type and overall DAs; physical neglect (PN) was predictive of attraction and repulsion-type DAs. Higher childhood trauma counts significantly predicted more types of DAs in the MDD group.

Conclusion: DAs are a trait feature of CM. EA and PN predict specific types of DAs in MDD patients. Higher CTCs predict more DAs in MDD patients.
Endocannabinoid System Unlocks the Puzzle of Autism Treatment via Microglia
Authors: Su Tangfeng , Yan Yu , Li Qiang , Ye Jiacai , Pei Lei
Published: October 2021

Autism spectrum disorder (ASD) is a serious neurodevelopmental disorder and characterized by early childhood-onset impairments in social interaction and communication, restricted and repetitive patterns of behavior or interests. So far there is no effective treatment for ASD, and the pathogenesis of ASD remains unclear. Genetic and epigenetic factors have been considered to be the main cause of ASD. It is known that endocannabinoid and its receptors are widely distributed in the central nervous system, and provide a positive and irreversible change toward a more physiological neurodevelopment. Recently, the endocannabinoid system (ECS) has been found to participate in the regulation of social reward behavior, which has attracted considerable attention from neuroscientists and neurologists. Both animal models and clinical studies have shown that the ECS is a potential target for the treatment of autism, but the mechanism is still unknown. In the brain, microglia express a complete ECS signaling system. Studies also have shown that modulating ECS signaling can regulate the functions of microglia. By comprehensively reviewing previous studies and combining with our recent work, this review addresses the effects of targeting ECS on microglia, and how this can contribute to maintain the positivity of the central nervous system, and thus improve the symptoms of autism. This will provide insights for revealing the mechanism and developing new treatment strategies for autism.
Prediction and Prevention in the Clinical High-Risk for Psychosis Paradigm: A Review of the Current Status and Recommendations for Future Directions of Inquiry
Authors: Worthington Michelle A. , Cannon Tyrone D.
Published: October 2021

Prediction and prevention of negative clinical and functional outcomes represent the two primary objectives of research conducted within the clinical high-risk for psychosis (CHR-P) paradigm. Several multivariable “risk calculator” models have been developed to predict the likelihood of developing psychosis, although these models have not been translated to clinical use. Overall, less progress has been made in developing effective interventions. In this paper, we review the existing literature on both prediction and prevention in the CHR-P paradigm and, primarily, outline ways in which expanding and combining these paths of inquiry could lead to a greater improvement in individual outcomes for those most at risk.
Relating Global Cognition With Upper-Extremity Motor Skill Retention in Individuals With Mild-to-Moderate Parkinson's Disease
Authors: Lingo VanGilder Jennapher , Lopez-Lennon Cielita , Paul Serene S. , Dibble Leland E. , Duff Kevin , Schaefer Sydney Y.
Published: October 2021

Background and Purpose:Cognition has been linked to rehabilitation outcomes in stroke populations, but this remains unexplored in individuals with Parkinson's disease (PD). The purpose of this secondary data analysis from a recent clinical trial (NCT02600858) was to determine if global cognition was related to skill performance after motor training in individuals with PD.

Methods:Twenty-three participants with idiopathic PD completed 3 days of training on an upper-extremity task. For the purposes of the original clinical trial, participants trained either “on” or “off” their dopamine replacement medication. Baseline, training, and 48-h retention data have been previously published. Global cognition was evaluated using the Montreal Cognitive Assessment (MoCA). Linear regression examined whether MoCA score predicted longer-term retention at nine-day follow-up; baseline motor task performance, age, PD severity, depressive symptoms, and group (medication “on”/“off”) were included as covariates. Baseline and follow-up motor task performance were assessed for all participants while “on” their medication.

Results:MoCA score was positively related to follow-up motor task performance, such that individuals with better cognition were faster than those with poorer cognition. Baseline task performance, age, PD severity, depressive symptoms, and medication status were unrelated to follow-up performance.

Discussion and Conclusions:Results of this secondary analysis align with previous work that suggest cognitive impairment may interfere with motor learning in PD and support the premise that cognitive training prior to or concurrent with motor training may enhance rehabilitative outcomes for individuals with PD. Findings also suggest that assessing cognition in individuals with PD could provide prognostic information about their responsiveness to motor rehabilitation.
Creating a Live and Flexible Normative Dataset for Netball
Authors: Croft Hayden , Spencer Kirsten , Taurua Noeline , Wilton Emily
Published: October 2021

A previous research has identified large data and information sources which exist about netball performance and align with the discussion of coaches during the games. Normative data provides context to measures across many disciplines, such as fitness testing, physical conditioning, and body composition. These data are normally presented in the tables as representations of the population categorized for benchmarking. Normative data does not exist for benchmarking or contextualization in netball, yet the coaches and players use performance statistics. A systems design methodology was adopted for this study where a process for automating the organization, normalization, and contextualization of netball performance data was developed. To maintain good ecological validity, a case study utilized expert coach feedback on the understandability and usability of the visual representations of netball performance population data. This paper provides coaches with benchmarks for assessing the performances of players, across competition levels against the player positions for performance indicators. It also provides insights to a performance analyst around how to present these benchmarks in an automated “real-time” reporting tool.
“Now the Forest Is Over”: Transforming the Commons and Remaking Gender in Cambodia's Uplands
Authors: Beban Alice , Bourke Martignoni Joanna
Published: October 2021

Communal lands and natural resources in rural Cambodia have transformed over the past 30 years as the country attempts to transition from conflict to liberal democracy and integrates into global agricultural value chains. We find that gender relations are changing as a result of land privatization and the ensuing social and ecological crises of production and reproduction. The forest has become a space for the articulation of new masculinities modulated through class and racialised power, while women are increasingly relegated to the private space of the home and village, negotiating expectations that they perform care, farming and food provisioning work while juggling household debt. We ground our argument in a large sample of qualitative interviews conducted between 2016 and 2020 in the upland provinces of Kampong Thom, Kratie and Ratanakiri that provide narrative accounts of the transformation of common forest and grazing lands, logging livelihoods and food provisioning practices. Using a feminist political ecology perspective, we highlight the contradictory processes of enclosure of the commons, which operate simultaneously as sites of violence, resistance, adaptation and continuity.
Management of Severe Traumatic Brain Injury: A Single Institution Experience in a Middle-Income Country
Authors: Basha Ahmed Kamel , Mahmoud Mohamed Ashraf , Al Ashwal Mohamed Ismail , Aglan Osama , ElShawady Sherif Bahaa , Abdel-Latif Assem Mounir , Elsayed Ahmed M. , AbdelGhany Walid
Published: October 2021

Introduction: Severe traumatic brain injury (TBI) is a major public health problem usually resulting in mortality or severe disabling morbidities of the victims. Intracranial pressure (ICP) monitoring is recently recognized as an imperative modality in the management of severe TBI, whereas growing evidence, based on randomized controlled trials (RCTs), suggests that ICP monitoring does not affect the outcome when compared with clinical and radiological data-based management. Also, ICP monitoring carries a considerable risk of intracranial infection that cannot be overlooked. The aim of this study is to assess the different aspects of our current local institutional management of severe TBI using non-invasive ICP monitoring for a potential need to change our management strategy.

Methods: We retrospectively reviewed our data of TBI from June 2019 through January 2020. Patients with severe TBI were identified. Their demographics, Glasgow coma score (GCS) at presentation, treatments received, and imaging data were extracted from the charts. Glasgow outcome scale extended (GOS-E) at 6 months was also assessed for the patients.

Results: Twenty patients with severe TBI were identified on chart review. Ten patients received only medical treatment measures to lower the ICP, whereas the other 10 patients had additional surgical interventions. In one patient, a ventriculostomy tube was inserted to monitor ICP and to drain cerebrospinal fluid (CSF). This was complicated by ventriculostomy-associated infection (VAI) and the tube was removed. In our cohort, the total mortality rate was 40%. The average GOS-E for the survivor patients managed without ICP monitoring based on the clinical and radiological data was 6.2 at 6 months follow-up. The 6-month overall good outcome, based on GOS-E, was 33.3%.

Conclusion: Although recent guidelines advocate for the use of ICP monitoring in the management of severe TBI, they remain underutilized in our practice due to many factors. External ventricular drains were mainly used to drain CSF; however, the higher rates of VAIs in our institution compared with the literature-reported rates are not in favor of the use of ICP monitoring. We recommend doing a comparative study between our current practice using clinical-and radiological-based management and subdural or intraparenchymal bolts. More structured RCTs are needed to validate these findings in our setting.
Postoperative Systemic Immune-Inflammation Index (SII): A Superior Prognostic Factor of Endometrial Cancer
Authors: Huang Yihong , Chen Yu , Zhu Yan , Wu Qing , Yao Chengyun , Xia Hongping , Li Congzhu
Published: October 2021

Objective: This study evaluates the preoperative and postoperative systemic immune-inflammation index (SII) capacity to predict the prognosis of patients with endometrial carcinoma after the operation and build a nomogram model to assist clinical practice.

Methods: The retrospective study included 362 consecutive patients with surgically resected endometrial cancer between January 2010 and June 2015 at The Affiliated Cancer Hospital of Shantou University Medical College. Blood routine was examined within 1 week before surgery to calculate SII, NLR, PLR, and MLR and 3 days after surgery to measure SII. The Pearson's χ2-test or Fisher's exact test was used to explore their relationship to clinical variables. The univariate and multivariate survival analyses were performed by Cox regression to identify the independent prognostic indicators. The Kaplan–Meier method with the log-rank test was used to generate the overall survival (OS) curves. R software was used to generate the receiver operating characteristic (ROC) curve and then it got the optimum cutoff value through the maximum Youden index. A nomogram model was formed with systemic immune inflammation and clinical factors.

Results: The preoperative SII was related to age (p = 0.009), FIGO stage (p = 0.02) and menopause (p = 0.014). The postoperative SII was associated with menopause (p = 0.014). Univariate analysis indicated that FIGO stage, lymphatic invasion, depth of myometrial invasion, postoperative chemotherapy, postoperative radiotherapy, preoperative SII, NLR, PLR, MLR, CRP, CA125, and postoperative SII were predictors of OS (p < 0.05). Multivariate analysis showed that lymphatic invasion and postoperative SII were independent prognostic factors of OS (p < 0.05). The nomogram model was visualized precisely to reflect the prognosis with a C-index value of 0.866 in this model.

Conclusion: The postoperative SII is the independent prognostic factor in patients with endometrial carcinoma after the operation and contributes to poor outcomes. However, after surgery, the preoperative SII and preoperative NLR, PLR, and MLR are not associated with OS endometrial carcinoma. Making good use of the nomogram model would contribute to better subsequent therapy.
Case Report: Cutaneous Pleomorphic Lymphangiosarcoma in a Dog Exhibiting Features of Human Composite Hemangioendothelioma
Authors: Cook Matthew R. , Lorbach Joshua N. , White Mary E. , Zann Geoffrey J. , Cianciolo Rachel E. , Selmic Laura E. , Wavreille Vincent , Kisseberth William C.
Published: October 2021

Background: Angiosarcomas are a broad category of vascular origin neoplasms that are poorly characterized in veterinary species. Lymphangiosarcoma (LAS) is an uncommon type of angiosarcoma reported in humans and canines arising from lymphatic endothelium. LAS can be differentiated from other angiosarcomas in dogs based on expression of Prospero-related homeobox gene-1 (PROX-1) or lymphatic vessel endothelial receptor-1 (LYVE-1). Composite hemangioendothelioma (CHE) is a rare angiosarcoma subtype described in people and characterized by a variable biologic behavior and infrequent metastasis. This variant of angiosarcoma histologically combines features of retiform hemangioendothelioma and epithelioid hemangioendothelioma. Information regarding the cytologic and histopathologic appearance and clinical course of dogs with vascular tumors that exhibit features of CHE are unknown. Here, we report a case of pleomorphic LAS with features of CHE arising in a dog and treated with surgery and adjuvant chemotherapy.

Case presentation: A 10-year-old intact male Labrador retriever presented with an approximately 6-cm-diameter cutaneous mass caudal to the left elbow that was progressively growing over 1.5 years. On physical examination, palpable extensions were identified coursing proximally over the triceps with concurrent loco-regional peripheral lymphadenopathy. Fine needle aspirates (FNA) and cytologic assessment of the cutaneous mass, left prescapular, and accessory axillary lymph nodes reported that this appeared to be a metastatic epithelial neoplasm, although a mixed carcinoma or collision tumor could not be excluded. An incisional biopsy of the mass was submitted for histopathology and was consistent with a well-differentiated angiosarcoma with features of CHE. The neoplasm expressed vimentin, CD31, von Willebrand factor (vWf), and PROX-1, supporting the diagnosis of LAS. Complete staging was performed, and no additional metastatic lesions were identified. Left forelimb amputation and lymph node removal were performed. Based on the diagnosis of metastatic LAS, doxorubicin chemotherapy was administered. 7 months post-amputation, the tumor recurred at the amputation site without evidence of metastatic disease.

Conclusion: This report describes a malignant, locally aggressive lymphatic origin vascular tumor in a dog, with features consistent with descriptions of CHE in humans. Cytologic features in this case were discordant with its true mesenchymal etiology, obfuscating diagnosis. The morphologic features of the mesenchymal neoplastic population and immunohistochemistry (IHC) labeling ultimately supported a diagnosis of pleomorphic LAS with features of CHE.
Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage
Authors: Ribas-Maynou Jordi , Llavanera Marc , Mateo-Otero Yentel , Garcia-Bonavila Estela , Delgado-Bermúdez Ariadna , Yeste Marc
Published: October 2021

Although sperm chromatin damage, understood as damage to DNA or affectations in sperm protamination, has been proposed as a biomarker for sperm quality in both humans and livestock, the low incidence found in some animals raises concerns about its potential value. In this context, as separate methods measure different facets of chromatin damage, their comparison is of vital importance. This work aims at analyzing eight techniques assessing chromatin damage in pig sperm. With this purpose, cryopreserved sperm samples from 16 boars were evaluated through the following assays: TUNEL, TUNEL with decondensation, SCSA, alkaline and neutral sperm chromatin dispersion (SCD) tests, alkaline and neutral Comet assays, and chromomycin A3 test (CMA3). In all cases, the extent of chromatin damage and the percentage of sperm with fragmented DNA were determined. The degree of chromatin damage and the percentage of sperm with fragmented DNA were significantly correlated (p < 0.05) in direct methods (TUNEL, TUNEL with decondensation, and alkaline and neutral Comet) and CMA3, but not in the indirect ones (SCD and SCSA). Percentages of sperm with fragmented DNA determined by alkaline Comet were significantly (p < 0.05) correlated with TUNEL following decondensation and CMA3; those determined by neutral Comet were correlated with the percentage of High DNA Stainability (SCSA); those determined by SCSA were correlated with neutral and alkaline SCD; and those determined by neutral SCD were correlated with alkaline SCD. While, in pigs, percentages of sperm with fragmented DNA are directly related to the extent of chromatin damage when direct methods are used, this is not the case for indirect techniques. Thus, the results obtained herein differ from those reported for humans in which TUNEL, SCSA, alkaline SCD, and alkaline Comet were found to be correlated. These findings may shed some light on the interpretation of these tests and provide some clues for the standardization of chromatin damage methods.
Changes in the Antioxidant and Mineral Status of Rabbits After Administration of Dietary Zinc and/or Thyme Extract
Authors: Kucková Katarína , Grešáková L'ubomíra , Takácsová Margaréta , Kandričáková Anna , Chrastinová L'ubica , Polačiková Mária , Cieslak Adam , Ślusarczyk Sylwester , Čobanová Klaudia
Published: October 2021

This study was aimed at determining the impact of organic zinc (Zn) and thyme extract (TE) administration, given alone or together for 6 weeks, on the antioxidant and mineral status (Zn, Cu, Fe, and Mn) in the plasma and tissues of growing rabbits. A total of 96 rabbits of age 35 days were randomly assigned to one of four treatment groups: a control group (C), a Zn group supplemented with dietary zinc (50 mg/kg), a TE group receiving thyme extract applied in drinking water (1 ml/L), and a Zn + TE group treated with both additives. Lipid peroxidation in the plasma was influenced by Zn intake and in the kidney was affected by both the Zn and TE treatment (P < 0.05). Zn supplementation led to a significant increase in glutathione peroxidase activity (P = 0.017), total antioxidant capacity (P = 0.009) and total thiol groups level (P = 0.047) in the kidney, with the highest values occurring in rabbits receiving the combination Zn + TE. Administration of TE influenced Zn content in the kidney (P < 0.001), while zinc intake decreased Cu concentration in muscle (P = 0.021). In conclusion, the simultaneous administration of organic Zn and TE positively affected the antioxidant response of kidneys and can be used for improving the antioxidant status of growing rabbits.
Model-Based Observer Design Considering Unequal Measurement Delays
Authors: Alipouri Yousef , Zhong Lexuan
Published: October 2021

State observers are essential components of a modern control system. It is often designed based on a mathematical model of the process, thus requiring detailed process knowledge. However, in the existing state estimation methods, equal delays are commonly assumed for all communication lines, which is unrealistic and poses problems such as instability and a degraded performance of observers when unequal time delays exist. In this paper, a design of observers considering the measurement delays is presented. To deal with this problem, a chain-based observer has been proposed in which each chain deals with one output delay, performs prediction for the unavailable output value, and passes it to the next chain. Convergence of each chain observer as well as overall state estimation were proven. To illustrate the performance of the proposed scheme, simulation studies were performed on a benchmark continuous stirred tank heater (CSTH) process.
An Agronomic Approach to Pine Nut Production by Grafting Stone Pine on Two Rootstocks
Authors: Guàrdia Mercè , Teixidó Anna , Sanchez-Bragado Rut , Aletà Neus
Published: October 2021

Stone pine (Pinus pinea) grows in natural stands within the Mediterranean basin and its nut is highly appreciated for its nutritional profile. Nevertheless, a decline in this species due to biotic and abiotic damages is currently being clearly observed. This situation has led to its development as a nut crop, to try to save its production and obtain regular harvests over the years. Under this agronomic scenario, the aim of this work was to compare the behavior of the stone pine grafted onto two rootstocks, P. pinea (PP) and P. halepensis (PH), by evaluating cone productivity, tree growth response, mast seeding patterns and pine nut composition. The field test was composed of 14 PH and 14 PP, randomly distributed into groups of 4–5 trees/rootstock. Data were from seven productive growing seasons. The results show higher growth and ripe cone production on PP rather than PH, although the productivity (cones/m2 canopy) was similar. Any effect of rootstock was observed on the mast seeding pattern and weight of cones, while the pine nut composition showed differences in the fatty acids content. The global quality of production was similar in PH and PP.
The Effects of Crops Together with Winter Cover Crops on the Content of Soil Water-Stable Aggregates in Organic Farming
Authors: Are Mihkel , Kaart Tanel , Selge Are , Reintam Endla
Published: October 2021

The stability of the soil aggregates is an important soil quality indicator, as it affects the soil’s overall functionality. As the soil aggregates are highly affected by agricultural practices, it is essential to know how crops interact with the aggregation process. Therefore, for obtaining more knowledge, this research was conducted in Estonia in an organic crop rotation field experiment from 2012/2013 through 2015/2016 to study the effects of crops (potato → spring barley undersown with red clover → red clover → winter wheat → pea) under different treatments (TC—control; TW—winter cover crops; TW+M—TW with farmyard manure 40 Mg ha−1 per crop rotation). The results showed that in the topsoil (5–10 cm), the soil water-stable aggregate (WSA) content (determined by the wet sieving method) from highest to lowest was following: pea (61.7%), winter wheat (61.6%), spring barley (61.5%), red clover (59.3%), potato (57.1%); whereas in the subsoil (30–35 cm): potato (50.6%), pea (48.5%), red clover (47.9%), spring barley (47.7%), winter wheat (46.4%). Therefore, potato was a noticeable crop, as among the crops, it had the lowest WSA content in the topsoil, while highest in the subsoil. The results shown gave an assumption that the after-effects of some crops (foremost with pea) were noticeable in the soil properties during the following crop. In the topsoil, the differences between crops were significant among crops just for TW and TW+M treatments. In TW, potato was lower than spring barley and winter wheat, but not significantly lower than pea or red clover. In the subsoil, significant differences between crops were observed for TC and TW treatments: in TC, potato was just significantly greater than red clover (but similar to other crops), and in TW, significantly greater than winter wheat. Furthermore, in the topsoil the soil organic carbon (SOC) content was not significantly affected by crops, and the use of winter cover crops generally increased the SOC content while concurrently decreased the WSA content and the soil maximum water holding capacity. This was probably caused by the additional tillage operations which cancelled out the possible benefits for the soil aggregates. As a consequence of the constantly declining SOC content, caused by the weakened soil aggregates, the plant-available P and K contents, especially in the absence of manure applications, decreased as well, probably due to the combination of fixation and removal of plant biomass. Therefore, it is expected that by continuing this trend, the plant growing conditions decline, which in turn will have a negative effect for the aggregate formation and carbon sequestration, which are essential for plant growth.
Profile of Semiquinone Radicals, Phytohormones and Sugars in Pistacia vera L. cv. Kirmizi Development
Authors: Morkunas Iwona , Doğu Mehmet Zafer , Woźniak Agnieszka , Bednarski Waldemar , Kęsy Jacek , Bocianowski Jan , Atar Şule Hilal , Ürün İpek Değirmenci , Labudda Mateusz , Zydlik Zofia , Kafkas Nesibe Ebru , Kafkas Salih , Jeandet Philippe
Published: October 2021

The main aim of this study was to investigate metabolic responses of fruits, leaves, and shoots of pistachio trees (Pistacia vera L. cv. Kirmizi) during their development. Electron paramagnetic resonance spectroscopy revealed significant increase in generation of semiquinone radicals in fruits and leaves of pistachio, while the flower cluster thinning application was conducted in relation to the control; especially at the second term of the plant material collection. Moreover, flower abscission caused an increase in the level of phytohormones such as indole-3-acetic acid and abscisic acid in fruits at the first term of fruits sampling. In turn, high-performance liquid chromatography analysis revealed differences both in the profile as well as the contents of soluble sugars detected in pistachio organs. The highest total sugar content was found in fruits of pistachio where the flower cluster thinning application was made early. Total sugar levels were higher also in leaves and shoots of the above-mentioned pistachio plants in relation to the control until the third sampling time. The importance of high levels of fructose in the fruits following the flower abscission was observed. Additionally, analyses of mineral elements in organs showed that copper and phosphorus contents in fruits were higher after the flower abscission in relation to the control. To conclude, our findings signal on contribution of semiquinone radicals, paramagnetic manganese ions, phytohormones, nutrients, and sugars in pistachio organs development on the background of the flower cluster thinning which was applied before fructification.
Soil and Plant Nutrient Analysis with a Portable XRF Probe Using a Single Calibration
Authors: Antonangelo João , Zhang Hailin
Published: October 2021

A portable X-ray fluorescence probe (pXRF) is a tool that is used to measure many elements quickly and efficiently in various samples, without any pretreatment. However, each type of sample generally requires different calibrations to be accurate. To overcome this, our work evaluated the efficacy of determining several elements in forage plant samples using the ‘Soil Nutrient and Metal’ calibration in a commercially available pXRF probe, envisioning that a single calibration can be used to measure samples of different matrixes. For this, the net intensity of the pXRF probe was determined in place of the concentration values that are obtained directly from measurements. Elemental concentrations (P, K, Ca, Mg, S, Cu, Fe, Zn, and Mn) from forage plant samples, collected across Oklahoma, US, were assessed in a representative number of ‘modeling’ and ‘validation’ (independent dataset) samples. Linear regression (LR) associated with the d-index, polynomial regression (PR), and power regression (PwR) were tested for predictions, producing many statistical parameters associated with the models that were used for comparison goals. The pXRF elemental data provided highly reliable predictions of K, S, Zn, and Mn regardless of the regression model. Although all models can be reliable in prediction of Ca and Fe concentrations, the PwR provided better root mean square error (RMSE) values. The predictions of Mg concentrations were less reliable, although highly significant; however, the P and Cu predictions were not acceptable. Our work successfully showed that, once established, a single calibration curve that covers a wide range of concentrations of several elements in soils and plant tissues enables both soil and plant samples to be analyzed. This suggests that manufacturers can develop a new calibration model for a commercially available pXRF probe that covers a wide variety of heterogeneous samples.