bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrating a Three-Level GIS Framework and a Graph Model to Track, Represent, and Analyze the Dynamic Activities of Tidal Flats
Authors: Xu Chao , Liu Weibo
Published: February 2021

Tidal flats (non-vegetated area) are soft-sediment habitats that are alternately submerged and exposed to the air by changeable tidal levels. The tidal flat dynamics research mainly utilizes the cell-level comparisons between the consecutive snapshots, but the in-depth study requires more detailed information of the dynamic activities. To better track, represent, and analyze tidal flats’ dynamic activities, this study proposes an integrated approach of a three-level Geographic Information Science (GIS) framework and a graph model. In the three-level GIS framework, the adjacent cells are assembled as the objects, and the objects on different time steps are linked as lifecycles by tracking the predecessor–successor relationships. Furthermore, eleven events are defined to describe the dynamic activities throughout the lifecycles. The graph model provides a better way to represent the lifecycles, and graph operators are utilized to facilitate the event analysis. The integrated approach is applied to tidal flats’ dynamic activities in the southwest tip of Florida Peninsula from 1984 to 2018. The results suggest that the integrated approach provides an effective way to track, represent, and analyze the dynamic activities of tidal flats, and it offers a novel perspective to examine other dynamic geographic phenomena with large spatiotemporal scales.
Neuraminidase Inhibitor Zanamivir Ameliorates Collagen-Induced Arthritis
Authors: Sehnert Bettina , Mietz Juliane , Rzepka Rita , Buchholz Stefanie , Maul-Pavicic Andrea , Schaffer Sandra , Nimmerjahn Falk , Voll Reinhard E.
Published: February 2021

Altered sialylation patterns play a role in chronic autoimmune diseases such as rheumatoid arthritis (RA). Recent studies have shown the pro-inflammatory activities of immunoglobulins (Igs) with desialylated sugar moieties. The role of neuraminidases (NEUs), enzymes which are responsible for the cleavage of terminal sialic acids (SA) from sialoglycoconjugates, is not fully understood in RA. We investigated the impact of zanamivir, an inhibitor of the influenza virus neuraminidase, and mammalian NEU2/3 on clinical outcomes in experimental arthritides studies. The severity of arthritis was monitored and IgG titers were measured by ELISA. (2,6)-linked SA was determined on IgG by ELISA and on cell surfaces by flow cytometry. Zanamivir at a dose of 100 mg/kg (zana-100) significantly ameliorated collagen-induced arthritis (CIA), whereas zana-100 was ineffective in serum transfer-induced arthritis. Systemic zana-100 treatment reduced the number of splenic CD138+/TACI+ plasma cells and CD19+ B cells, which was associated with lower IgG levels and an increased sialylation status of IgG compared to controls. Our data reveal the contribution of NEU2/3 in CIA. Zanamivir down-modulated the T and B cell-dependent humoral immune response and induced an anti-inflammatory milieu by inhibiting sialic acid degradation. We suggest that neuraminidases might represent a promising therapeutic target for RA and possibly also for other antibody-mediated autoimmune diseases.
The Multifunctional Roles of Polyphenols in Plant-Herbivore Interactions
Authors: Singh Sukhman , Kaur Ishveen , Kariyat Rupesh
Published: February 2021

There is no argument to the fact that insect herbivores cause significant losses to plant productivity in both natural and agricultural ecosystems. To counter this continuous onslaught, plants have evolved a suite of direct and indirect, constitutive and induced, chemical and physical defenses, and secondary metabolites are a key group that facilitates these defenses. Polyphenols—widely distributed in flowering plants—are the major group of such biologically active secondary metabolites. Recent advances in analytical chemistry and metabolomics have provided an opportunity to dig deep into extraction and quantification of plant-based natural products with insecticidal/insect deterrent activity, a potential sustainable pest management strategy. However, we currently lack an updated review of their multifunctional roles in insect-plant interactions, especially focusing on their insect deterrent or antifeedant properties. This review focuses on the role of polyphenols in plant-insect interactions and plant defenses including their structure, induction, regulation, and their anti-feeding and toxicity effects. Details on mechanisms underlying these interactions and localization of these compounds are discussed in the context of insect-plant interactions, current findings, and potential avenues for future research in this area.
Tumor Necrosis Factor-Like Weak Inducer of Apoptosis and Selected Cytokines—Potential Biomarkers in Children with Solitary Functioning Kidney
Authors: Nosek Hanna , Jankowska Dorota , Brzozowska Karolina , Kazberuk Katarzyna , Wasilewska Anna , Taranta-Janusz Katarzyna
Published: February 2021

This study was performed to explore serum tumor necrosis factor-like weak inducer of apoptosis (TWEAK) and its dependent cytokines urinary excretion: monocyte chemoattractant protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted chemokine (RANTES) with their relation to the kidney function parameters in children with solitary functioning kidney (SFK). The study included 80 children and adolescents (median age 9.75 year) with congenital and acquired (after surgical removal) SFK. Serum TWEAK and urinary MCP-1 and RANTES levels were significantly higher in SFK patients (p < 0.05). The serum TWEAK was positively related to serum creatinine (r = 0.356; p < 0.001). Moreover, in SFK the receiver operating characteristic analyses revealed good diagnostic profile for serum TWEAK with AUC (Area Under The Curve)—0.853, uRANTES—0.757, and for RANTES/cr.: AUC—0.816. Analysis carried out to identify children with impaired renal function (albuminuria and/or decreased estimated glomerular filtration rate < 90 mL/min/1.73 m2 and/or hypertension) showed good profile for TWEAK (AUC—0.79) and quite good profile for uRANTES and RANTES/cr. (AUC 0.66 and 0.631, respectively). This is the first study investigating serum TWEAK and urinary excretion of MCP-1 and RANTES together in children with SFK. Obtained results indicate that TWEAK and RANTES may serve as potential markers of renal impairment.
Towards an Enhanced Tool for Quantifying the Degree of LV Hyper-Trabeculation
Authors: Bernabé Gregorio , Casanova José D. , González-Carrillo Josefa , Gimeno-Blanes Juan R.
Published: February 2021

Left ventricular non-compaction (LVNC) is defined by an increase of trabeculations in left ventricular (LV) endomyocardium. Although LVNC can be in isolation, an increase in hypertrabeculation often accompanies genetic cardiomyopathies. Current methods for quantification of LV trabeculae have limitations. Several improvements are proposed and implemented to enhance a software tool to quantify the trabeculae degree in the LV myocardium in an accurate and automatic way for a population of patients with genetic cardiomyopathies (QLVTHCI). The software tool is developed and evaluated for a population of 59 patients (470 end-diastole cardiac magnetic resonance images). This tool produces volumes of the compact sector and the trabecular area, the proportion between these volumes, and the left ventricular and trabeculated masses. Substantial enhancements are obtained over the manual process performed by cardiologists, so saving important diagnosis time. The parallelization of the detection of the external layer is proposed to ensure real-time processing of a patient, obtaining speed-ups from 7.5 to 1500 with regard to QLVTHCI and the manual process used traditionally by cardiologists. Comparing the method proposed with the fractal proposal to differentiate LVNC and non-LVNC patients among 27 subjects with previously diagnosed cardiomyopathies, QLVTHCI presents a full diagnostic accuracy, while the fractal criteria achieve 78%. Moreover, QLTVHCI can be installed and integrated in hospitals on request, whereas the high cost of the license of the fractal method per year of this tool has prevented reproducibility by other medical centers.
Impact of Canagliflozin in Patients with Type 2 Diabetes after Hospitalization for Acute Heart Failure: A Cohort Study
Authors: Martín Ernesto , López-Aguilera José , González-Manzanares Rafael , Anguita Manuel , Gutiérrez Guillermo , Luque Aurora , Paredes Nick , Oneto Jesús , Perea Jorge , Castillo Juan Carlos
Published: February 2021

Background: Heart failure (HF) is one of the mayor contributors to cardiovascular morbidity and mortality in patients with diabetes. Sodium-glucose cotransporter 2 (SGLT2) inhibitors have demonstrated to reduce the risk of hospitalization for HF in patients with type 2 diabetes mellitus (T2D). We aimed to assess the risk for re-hospitalization in a cohort of patients hospitalized for HF according to whether or not they received canagliflozin at discharge, as well as changes in N-terminal pro–B-type natriuretic peptide (NT-ProBNP) concentration during follow-up. Methods: We conducted a retrospective longitudinal study at a tertiary centre including 102 consecutive T2D patients discharged for acute HF without contraindication for SGLT2 inhibitors. We compared adverse clinical events (HF rehospitalization and cardiovascular death) and NT-ProBNP changes according to canagliflozin prescription at discharge. Results: Among the 102 patients included, 45 patients (44.1%) were prescribed canagliflozin and the remaining 57 (55.9%) were not prescribed any SGLT2 inhibitors (control group). After a median follow-up of 22 months, 45 patients (44.1%) were hospitalized for HF. Most of the rehospitalizations occurred during the first year (37.3%). HF readmission at first year occurred in 10 patients (22.2%) in the canagliflozin group and 29 patients (49.1%) in the control group (hazard ratio (HR): 0.45; 95% confidence interval (CI): 0.21–0.96; p < 0.039). A composite outcome of hospitalization for HF or death from cardiovascular causes was lower in the canagliflozin group (37.8%) than in the control group (70.2%) (HR: 0.51; 95% CI: 0.27–0.95; p < 0.035). Analysis of NT-ProBNP concentration showed an interaction between canagliflozin therapy and follow-up time (p = 0.002). Conclusions: Canagliflozin therapy at discharge was associated with a lower risk of readmission for HF and a reduction in NT-ProBNP concentration in patients with diabetes after hospitalization for HF.
Incoherence between Systemic Hemodynamic and Microcirculatory Response to Fluid Challenge in Critically Ill Patients
Authors: De Santis Paolo , De Fazio Chiara , Franchi Federico , Bond Ottavia , Vincent Jean-Louis , Creteur Jacques , Taccone Fabio Silvio , Scolletta Sabino
Published: February 2021

Background: The aim of the study was to assess the coherence between systemic hemodynamic and microcirculatory response to a fluid challenge (FC) in critically ill patients. Methods: We prospectively collected data in patients requiring a FC whilst cardiac index (CI) and microcirculation were monitored. The sublingual microcirculation was assessed using the incident dark field (IDF) CytoCam device (Braedius Medical, Huizen, The Netherlands). The proportion of small perfused vessels (PPV) was calculated. Fluid responders were defined by at least a 10% increase in CI during FC. Responders according to changes in microcirculation were defined by at least 10% increase in PPV at the end of FC. Cohen’s kappa coefficient was measured to assess the agreement to categorize patients as “responders” to FC according to CI and PPV. Results: A total of 41 FC were performed in 38 patients, after a median time of 1 (0–1) days after ICU admission. Most of the fluid challenges (39/41, 95%) were performed using crystalloids and the median total amount of fluid was 500 (500–500) mL. The main reasons for fluid challenge were oliguria (n = 22) and hypotension (n = 10). After FC, CI significantly increased in 24 (58%) cases; a total of 19 (46%) FCs resulted in an increase in PPV. Both CI and PPV increased in 13 responders and neither in 11; the coefficient of agreement was only 0.21. We found no correlation between absolute changes in CI and PPV after fluid challenge. Conclusions: The results of this heterogenous population of critically ill patients suggest incoherence in fluid responsiveness between systemic and microvascular hemodynamics; larger cohort prospective studies with adequate a priori sample size calculations are needed to confirm these findings.
Osteonecrosis of the Femoral Head Safely Healed with Autologous, Expanded, Bone Marrow-Derived Mesenchymal Stromal Cells in a Multicentric Trial with Minimum 5 Years Follow-Up
Authors: Gómez-Barrena Enrique , Padilla-Eguiluz Norma , Rosset Philippe , Hernigou Philippe , Baldini Nicola , Ciapetti Gabriela , Gonzalo-Daganzo Rosa , Avendaño-Solá Cristina , Rouard Hélène , Giordano Rosaria , Dominici Massimo , Schrezenmeier Hubert , Layrolle Pierre ,   on behalf of the REBORNE Consortium
Published: February 2021

Background: Osteonecrosis (ON) of the femoral head represents a potentially severe disease of the hip where the lack of bone regeneration may lead to femoral head collapse and secondary osteoarthritis, with serious pain and disability. The aim of this European, multicentric clinical trial was to prove safety and early efficacy to heal early femoral head ON in patients through minimally invasive surgical implantation of autologous mesenchymal stromal cells (MSC) expanded from bone marrow (BM) under good manufacturing practices (GMP). Methods: Twenty-two patients with femoral head ON (up to ARCO 2C) were recruited and surgically treated in France, Germany, Italy and Spain with BM-derived, expanded autologous MSC (total dose 140 million MSC in 7 mL). The investigational advanced therapy medicinal product (ATMP) was expanded from BM under the same protocol in all four countries and approved by each National Competent Authority. Patients were followed during two years for safety, based on adverse events, and for efficacy, based on clinical assessment (pain and hip score) and imaging (X-rays and MRIs). Patients were also reviewed after 5 to 6 years at latest follow-up for final outcome. Results: No severe adverse event was recalled as related to the ATMP. At 12 months, 16/20 per protocol and 16/22 under intention-to-treat (2 drop-out at 3 and 5 months) maintained head sphericity and showed bone regeneration. Of the 4 hips with ON progression, 3 required total hip replacement (THR). At 5 years, one patient (healed at 2 years visit) was not located, and 16/21 showed no progression or THR, 4/21 had received THR (all in the first year) and 1 had progressed one stage without THR. Conclusions: Expanded MSCs implantation was safe. Early efficacy was confirmed in 80% of cases under protocol at 2 years. At 5 years, the overall results were maintained and 19% converted to THR, all in the first year.
Dose Optimization of Tyrosine Kinase Inhibitors in Chronic Myeloid Leukemia: A New Therapeutic Challenge
Authors: Iurlo Alessandra , Cattaneo Daniele , Bucelli Cristina , Breccia Massimo
Published: February 2021

The chronic myeloid leukemia (CML) therapeutic landscape has dramatically changed with tyrosine kinase inhibitor (TKI) development, which allows a near-normal life expectancy. However, long-term TKI exposure has been associated with persistent adverse events (AEs) which negatively impact on quality of life (QoL) and have the potential to cause significant morbidity and mortality. In clinical practice, TKI dose reduction is usually considered to reduce AEs and improve QoL, but dose optimization could have also another aim, i.e., the achievement and maintenance of cytogenetic and molecular responses. While therapy cessation appeared as a safe option for about half of the patients achieving an optimal response, no systematic assessment of long-term TKI dose de-escalation has been made. The present review is focused on the most recent evidences for TKIs dose modifications in CML clinical studies and in the real-life setting. It will consider TKI dose modifications in newly diagnosed patients, dose reduction for AEs, or in deep molecular response, either as a prelude to treatment-free remission (TFR) or as continuous maintenance therapy in those patients not wishing to attempt TFR. In addition, it will focus on patients not achieving a molecular response deep enough to go to TFR, and for whom dose reduction could be an option to avoid AEs.
External Validation of Surrogate Indices of Fatty Liver in the General Population: The Bagnacavallo Study
Authors: Foschi Francesco , Conti Fabio , Domenicali Marco , Giacomoni Pierluigi , Borghi Alberto , Bevilacqua Vittoria , Napoli Lucia , Berardinelli Dante , Altini Mattia , Cucchetti Alessandro , Ercolani Giorgio , Casadei-Gardini Andrea , Bellentani Stefano , Gastaldelli Amalia , Tiribelli Claudio , Bedogni Giorgio ,   Bagnacavallo Study Group
Published: February 2021

We externally validated the fatty liver index (FLI), the lipid accumulation product (LAP), the hepatic steatosis index (HSI), and the Zhejiang University index (ZJU) for the diagnosis of fatty liver (FL) and non-alcoholic fatty liver disease (NAFLD) in the general population. The validation was performed on 2159 citizens of the town of Bagnacavallo (Ravenna, Italy). Calibration was evaluated by calculating the calibration slope and intercept and by inspecting calibration plots; discrimination was evaluated using the c-statistic. The average calibration slope was 1 and the average intercept was 0 for all combinations of outcomes and indices. For the diagnosis of FL, the c-statistic was 0.85 for FLI, 0.83 for ZJU, 0.82 for HSI, and 0.80 for LAP; for the diagnosis of NAFLD, the c-statistic was 0.77 for FLI, 0.76 for ZJU, 0.75 for HSI, and 0.74 for LAP. All indices were strongly correlated with each other. In conclusion, FLI, LAP, HSI, and ZJU perform similarly well to diagnose FL and NAFLD in the Bagnacavallo population, even if FLI has a small advantage as discrimination is concerned.
Interactions of Cd2+, Co2+ and MoO42− Ions with Crushed Concrete Fines
Authors: Elmes Victoria K. , Coleman Nichola J.
Published: February 2021

Construction and demolition activities generate approximately two thirds of the world’s waste, with concrete-based demolition material accounting for the largest proportion. Primary aggregates are recovered and reused, although the cement-rich fine fraction is underutilised. In this study, single metal batch sorption experiments confirmed that crushed concrete fines (CCF) are an effective sorbent for the maximum exclusion of 45.2 mg g−1 Cd2+, 38.4 mg g−1 Co2+ and 56.0 mg g−1 MoO42− ions from aqueous media. The principal mechanisms of sorption were determined, by scanning electron microscopy of the metal-laden CCF, to be co-precipitation with Ca2+ ions released from the cement to form solubility limiting phases. The removal of Co2+ and MoO42− ions followed a zero-order reaction and that of Cd2+ was best described by a pseudo-second-order model. The Langmuir model provided the most appropriate description of the steady state immobilisation of Cd2+ and Co2+, whereas the removal of MoO42− conformed to the Freundlich isotherm. Long equilibration times (>120 h), loose floc formation and high pH are likely to limit the use of CCF in many conventional wastewater treatment applications; although, these properties could be usefully exploited in reactive barriers for the management of contaminated soils, sediments and groundwater.
Drastic Reduction of Bacterial, Fungal and Viral Pathogen Titers by Cuprous Oxide Impregnated Medical Textiles
Authors: Borkow Gadi , Salvatori Rachel , Kanmukhla Vikram K.
Published: February 2021

Hospital patients and personnel are at risk of nosocomial viral infections, as clearly manifested during the COVID-19 pandemic. Transmission of respiratory viral pathogens can occur through contaminated surfaces, including from medical textiles. Copper has potent biocidal properties, and cuprous oxide impregnated medical textiles (CMT) reduce hospital-acquired bacterial infections. In the current study we confirm the antimicrobial properties of CMT and determine their capacity to reduce infectious titres of human coronavirus (HCoV-229E) in an independent laboratory. The antibacterial and antiviral activities of the CMT were determined according to AATCC TM100-2019 and ISO 18184:2019 standards, respectively. The CMT reduced by 4 logs the viable titers of MRSA, Klebsiella pneumoniae, Enterococcus faecalis, and Candida auris after 2 h of incubation. Viable titers of Clostridium difficile were reduced by 2.3, 3, and 4 logs after 2, 6, and 18 h, respectively. Infectious titers of HCoV-229E exposed to CMT for 2 h were reduced by 2.8 and 4 logs (99.85% and 99.99% reductions) as compared to Time-0 control and initial inoculum, respectively. The CMT retain their antibacterial efficacy even after 100 industrial washings. Use of cuprous oxide impregnated textiles in clinical settings may reduce not only hospital acquired infections caused by bacterial and fungal pathogens, but also, and equally important, those caused by coronavirus and other viruses.
Enhanced Region Growing for Brain Tumor MR Image Segmentation
Authors: Biratu Erena Siyoum , Schwenker Friedhelm , Debelee Taye Girma , Kebede Samuel Rahimeto , Negera Worku Gachena , Molla Hasset Tamirat
Published: February 2021

A brain tumor is one of the foremost reasons for the rise in mortality among children and adults. A brain tumor is a mass of tissue that propagates out of control of the normal forces that regulate growth inside the brain. A brain tumor appears when one type of cell changes from its normal characteristics and grows and multiplies abnormally. The unusual growth of cells within the brain or inside the skull, which can be cancerous or non-cancerous has been the reason for the death of adults in developed countries and children in under developing countries like Ethiopia. The studies have shown that the region growing algorithm initializes the seed point either manually or semi-manually which as a result affects the segmentation result. However, in this paper, we proposed an enhanced region-growing algorithm for the automatic seed point initialization. The proposed approach’s performance was compared with the state-of-the-art deep learning algorithms using the common dataset, BRATS2015. In the proposed approach, we applied a thresholding technique to strip the skull from each input brain image. After the skull is stripped the brain image is divided into 8 blocks. Then, for each block, we computed the mean intensities and from which the five blocks with maximum mean intensities were selected out of the eight blocks. Next, the five maximum mean intensities were used as a seed point for the region growing algorithm separately and obtained five different regions of interest (ROIs) for each skull stripped input brain image. The five ROIs generated using the proposed approach were evaluated using dice similarity score (DSS), intersection over union (IoU), and accuracy (Acc) against the ground truth (GT), and the best region of interest is selected as a final ROI. Finally, the final ROI was compared with different state-of-the-art deep learning algorithms and region-based segmentation algorithms in terms of DSS. Our proposed approach was validated in three different experimental setups. In the first experimental setup where 15 randomly selected brain images were used for testing and achieved a DSS value of 0.89. In the second and third experimental setups, the proposed approach scored a DSS value of 0.90 and 0.80 for 12 randomly selected and 800 brain images respectively. The average DSS value for the three experimental setups was 0.86.
Impact of Management Regime and Regime Change on Gravel Barrier Response to a Major Storm Surge
Authors: Pollard James A. , Christie Elizabeth K. , Brooks Susan M. , Spencer Tom
Published: February 2021

Gravel barriers represent physiographic, hydrographic, sedimentary, and ecological boundaries between inshore and open marine offshore environments, where they provide numerous important functions. The morphosedimentary features of gravel barriers (e.g., steep, energy reflective form) have led to their characterization as effective coastal defense features during extreme hydrodynamic conditions. Consequently, gravel barriers have often been intensively managed to enhance coastal defense functions. The Blakeney Point Barrier System (BPBS), U.K., is one such example, which offers the opportunity to investigate the impact of alternative management regimes under extreme hydrodynamic conditions. The BPBS was actively re-profiled along its eastern section from the 1950s to the winter of 2005, whilst undergoing no active intervention along its western section. Combining an analysis of remotely sensed elevation datasets with numerical storm surge modeling, this paper finds that interventionist management introduces systemic differences in barrier morphological characteristics. Overly steepened barrier sections experience greater wave run-up extents during storm surge conditions, leading to more extreme morphological changes and landward barrier retreat. Furthermore, while high, steep barriers can be highly effective at preventing landward flooding, in cases where overwashing does occur, the resultant landward overtopping volume is typically higher than would be the case for a relatively lower crested barrier with a lower angled seaward slope. There is a growing preference within coastal risk management for less interventionist management regimes, incorporating natural processes. However, restoring natural processes does not immediately or inevitably result in a reduction in coastal risk. This paper contributes practical insights regarding the time taken for a previously managed barrier to relax to a more natural state, intermediary morphological states, and associated landward water flows during extreme events, all of which should be considered if gravel barriers are to be usefully integrated into broader risk management strategies.
Longitudinal Evaluation of Plasma Cytokine Levels in Patients with Invasive Candidiasis
Authors: Wunsch Stefanie , Zurl Christoph , Strohmaier Heimo , Meinitzer Andreas , Rabensteiner Jasmin , Posch Wilfried , Lass-Flörl Cornelia , Cornely Oliver , Pregartner Gudrun , König Elisabeth , Feierl Gebhard , Hoenigl Martin , Prattes Juergen , Zollner-Schwetz Ines , Valentin Thomas , Krause Robert
Published: February 2021

Interleukin (IL) 17A plays a decisive role in anti-Candida host defense. Previous data demonstrated significantly increased IL-17A values in candidemic patients. We evaluated levels and time courses of IL-17A, and other cytokines suggested to be involved in Candida-specific immunity (IL-6, IL-8, IL-10, IL-17F, IL-22, IL-23, interferon-γ, tumor necrosis factor-α, Pentraxin-related protein 3, transforming growth factor-β) in patients with invasive candidiasis (IC) compared to bacteremic patients (Staphylococcus aureus, Escherichia coli) and healthy controls (from previous 4 days up to day 14 relative to the index culture (−4; 14)). IL-17A levels were significantly elevated in all groups compared to healthy controls. In IC, the highest IL-17A values were measured around the date of index sampling (−1; 2), compared to significantly lower levels prior and after sampling the index culture. Candidemic patients showed significantly higher IL-17A values compared to IC other than candidemia at time interval (−1; 2) and (3; 7). No significant differences in IL-17A levels could be observed for IC compared to bacteremic patients. Candidemic patients had higher IL-8, IL-10, IL-22, IFN-γ, PTX3 and TNF-α values compared to non-candidemic. Based on the limited discriminating competence between candidemia and bacteremia, IL-17A has to be considered a biomarker for blood stream infection rather than invasive Candida infection.
Identifying the Necessities of Regional-Based Analysis to Study Germany’s Biogas Production Development under Energy Transition
Authors: Yang Xueqing , Liu Yang , Wang Mei , Bezama Alberto , Thrän Daniela
Published: February 2021

The German Renewable Energy Sources Act (EEG) has been deemed successful in promoting German biogas production. However, the German state-level biogas production development (BPD) under the EEG has not been systematically studied and compared. This research aimed to study the German state-level BPD using the multivariate linear regression model with a dummy variable, and to spatially quantify the environmental and agricultural consequences using the geographic information system (GIS) technique to identify the necessities of regional-based analysis on Germany’s BPD. The empirical results indicated that Saxony-Anhalt was advanced in BPD, while farmers’ response from Bavaria to EEG was the weakest. The reason behind could be the differences in farmers’ personality traits and risk cognitions toward the biogas production investment. The spatial analysis indicated that Saxony-Anhalt had more severe environmental problems caused by the biogas production expansion than Bavaria. Therefore, to promote BPD in states such as Bavaria, an increase in the nationwide unified subsidy might lead to an overreaction of the EEG strong response states, e.g., Saxony-Anhalt, leading to more serious environmental problems. In the end, there is a need for more regional-based research on studying the BPD in Germany in the future to avoid the ambiguity of large-scale studies.
Could the Calcium Silicate-Based Sealer Presentation Form Influence Dentinal Sealing? An In Vitro Confocal Laser Study on Tubular Penetration
Authors: Muedra Paula , Forner Leopoldo , Lozano Adrián , Sanz José L. , Rodríguez-Lozano Francisco J. , Guerrero-Gironés Julia , Riccitiello Francesco , Spagnuolo Gianrico , Llena Carmen
Published: February 2021

Dentinal tubule penetration influences root canal treatment sealing. The aim of this study was to compare dentinal penetration of two clinical presentations of silicate-based sealers using confocal laser. Sixty single-rooted human teeth from 50–70 year-old patients extracted for orthodontic/periodontal reasons were used. Canals were prepared using Mtwo system up to 35/0.04, with 5.25% NaOCl irrigation and final irrigation using 17% EDTA. Teeth were randomly assigned into study groups (ni = 20): EndoSequence BC sealer (ES, group 1), BioRoot RCS (BR, group 2); and a control group (nc = 20) with AH Plus (AHP). Root canals were obturated with 35/0.04 gutta-percha (single-cone technique). The samples were obtained from apical, middle, and coronal thirds. Dentinal tubule penetration depth and percentage of penetration around the canal perimeter were measured. The statistical analysis was performed using Mann Whitney U test and Wilcoxon t-test (95% confidence interval). ES exhibited a significantly higher penetration than AHP in apical and middle thirds (p < 0.05), and in middle and coronal thirds relative to BR (p < 0.05). The percentage of penetration around the canal perimeter was significantly higher for ES compared to BR in all thirds, but only in the apical third for AHP (p < 0.05). The pre-mixed silicate-based sealer exhibited better penetration than the powder/liquid one.
Ultrananocrystalline Diamond Nanowires: Fabrication, Characterization, and Sensor Applications
Authors: Zhou Andrew F. , Wang Xinpeng , Pacheco Elluz , Feng Peter X.
Published: February 2021

The aim of this review is to provide a survey of the recent advances and the main remaining challenges related to the ultrananocrystalline diamond (UNCD) nanowires and other nanostructures which exhibit excellent capability as the core components for many diverse novel sensing devices, due to the unique material properties and geometry advantages. The boron or nitrogen doping introduced in the gas phase during deposition promotes p-type or n-type conductivity. With the establishment of the UNCD nanofabrication techniques, more and more nanostructure-based devices are being explored in measuring basic physical and chemical parameters via classic and quantum methods, as exemplified by gas sensors, ultraviolet photodetectors, piezoresistance effect-based devices, biological applications and biosensors, and nitrogen-vacancy color center-based magnetic field quantum sensors. Highlighted finally are some of the remaining challenges and the future outlook in this area.
Coaxial Monitoring of AISI 316L Thin Walls Fabricated by Direct Metal Laser Deposition
Authors: Errico Vito , Campanelli Sabina , Angelastro Andrea , Dassisti Michele , Mazzarisi Marco , Bonserio Cesare
Published: February 2021

Direct metal laser deposition (DMLD) is an additive manufacturing technique suitable for coating and repair, which has been gaining a growing interest in 3D manufacturing applications in recent years. However, its diffusion in the manufacturing industry is still limited due to technical challenges to be solved—both the sub-optimal quality of the final parts and the low repeatability of the process make the DMLD inadequate for high-value applications requiring high-performance standards. Thus, real-time monitoring and process control are indispensable requirements for improving the DMLD process. The aim of this study was the optimization of deposition strategies for the fabrication of thin walls in AISI 316L stainless steel. For this purpose, a coaxial monitoring system and image processing algorithms were employed to study the melt pool geometry. The comparison tests carried out highlighted how the region-based active contour algorithm used for image processing is more efficient and stable than others covered in the literature. The results allowed the identification of the best deposition strategy. Therefore, it is shown how this monitoring methodology proved to be suitable for designing and implementing the right building strategy for DMLD manufactured 3D components. A fast and stable image processing method was achieved, which can be considered for future closed-loop monitoring in real-time applications.
Preparation and Tensile Properties of Novel Porous Plates Made by Stainless Steel Wire Mesh and Powder Composites
Authors: Lin Shengcun , Zhou Zhaoyao
Published: February 2021

Porous metal materials have important mechanical properties, and there are various manufacturing methods to produce them. In this paper, a porous, thin strip was fabricated by the composite rolling of stainless steel wire mesh and stainless steel powder. Then, a porous plate of stainless steel wire mesh and powder composite (SWMPC) was prepared by folding, pressing, and vacuum sintering the thin strip, and its structural characteristics and permeability were studied. The effects of the gap of the roller, gap of the powder box, number of layers by folding, and sintering parameters on the porosity and mechanical properties were also studied. The results indicated that the permeability increased with the increasing of porosity. Sintering parameters had a great influence on the mechanical properties. The larger the roll gap, the higher the porosity and the weaker the mechanical properties. As the gap of the powder box increased, the porosity decreased and the mechanical properties improved. The number of layers had no effect on the porosity. The first three stages of tensile curves of 10 and 15 layers were deformation stages and generally coincided, the time was short at the fracture stage. However, the mechanical properties got a raise when layers was 15.
Comparing Bayesian Spatial Conditional Overdispersion and the Besag–York–Mollié Models: Application to Infant Mortality Rates
Authors: Morales-Otero Mabel , Núñez-Antón Vicente
Published: February 2021

In this paper, we review overdispersed Bayesian generalized spatial conditional count data models. Their usefulness is illustrated with their application to infant mortality rates from Colombian regions and by comparing them with the widely used Besag–York–Mollié (BYM) models. These overdispersed models assume that excess of dispersion in the data may be partially caused from the possible spatial dependence existing among the different spatial units. Thus, specific regression structures are then proposed both for the conditional mean and for the dispersion parameter in the models, including covariates, as well as an assumed spatial neighborhood structure. We focus on the case of response variables following a Poisson distribution, specifically concentrating on the spatial generalized conditional normal overdispersion Poisson model. Models were fitted by making use of the Markov Chain Monte Carlo (MCMC) and Integrated Nested Laplace Approximation (INLA) algorithms in the specific context of Bayesian estimation methods.
A Discussion of a Cryptographical Scheme Based in F-Critical Sets of a Latin Square
Authors: Johnson Laura M. , Perkins Stephanie
Published: February 2021

This communication provides a discussion of a scheme originally proposed by Falcón in a paper entitled “Latin squares associated to principal autotopisms of long cycles. Applications in cryptography”. Falcón outlines the protocol for a cryptographical scheme that uses the F-critical sets associated with a particular Latin square to generate access levels for participants of the scheme. Accompanying the scheme is an example, which applies the protocol to a particular Latin square of order six. Exploration of the example itself, revealed some interesting observations about both the structure of the Latin square itself and the autotopisms associated with the Latin square. These observations give rise to necessary conditions for the generation of the F-critical sets associated with certain autotopisms of the given Latin square. The communication culminates with a table which outlines the various access levels for the given Latin square in accordance with the scheme detailed by Falcón.
Computer Vision and Human Behaviour, Emotion and Cognition Detection: A Use Case on Student Engagement
Authors: Vanneste Pieter , Oramas José , Verelst Thomas , Tuytelaars Tinne , Raes Annelies , Depaepe Fien , Van den Noortgate Wim
Published: February 2021

Computer vision has shown great accomplishments in a wide variety of classification, segmentation and object recognition tasks, but tends to encounter more difficulties when tasks require more contextual assessment. Measuring the engagement of students is an example of such a complex task, as it requires a strong interpretative component. This research describes a methodology to measure students’ engagement, taking both an individual (student-level) and a collective (classroom) approach. Results show that students’ individual behaviour, such as note-taking or hand-raising, is challenging to recognise, and does not correlate with students’ self-reported engagement. Interestingly, students’ collective behaviour can be quantified in a more generic way using measures for students’ symmetry, reaction times and eye-gaze intersections. Nonetheless, the evidence for a connection between these collective measures and engagement is rather weak. Although this study does not succeed in providing a proxy of students’ self-reported engagement, our approach sheds light on the needs for future research. More concretely, we suggest that not only the behavioural, but also the emotional and cognitive component of engagement should be captured.
Price Competition and Setup Cost
Authors: Ota Rui , Fujiu Hiroshi
Published: February 2021

Few studies analyze the endogenous emergence of price competition in a new product market. This paper analyzes two differentiated products, an existing product and a newly introduced substitutable product, and investigates conditions under which a price competition endogenously emerges in a new product market in the context of a choice between engaging in price competition and holding price leadership. We demonstrate that Bertrand price competition emerges when the setup cost for the new product is high enough. This result implies that government policies reducing setup costs such as subsidies could change the type of competition to price leadership in a new product market.
Prevalence of Upper Extremity Musculoskeletal Disorders in Patients with Type 2 Diabetes in General Practice
Authors: Alabdali Login Ahmed S. , Jaeken Jasmien , Dinant Geert-Jan , van den Akker Marjan , Winkens Bjorn , Ottenheijm Ramon P. G.
Published: February 2021

Background: One of the lesser recognized complications of diabetes mellitus are musculoskeletal (MSK) complications of the upper and lower extremity. No prevalence studies have been conducted in general practice. Thus, the aim of this study was to investigate the prevalence of upper extremity MSK disorders in patients with type 2 diabetes (T2DM) in the Netherlands. Methods: We conducted a cross-sectional study with two different approaches, namely a representative Dutch primary care medical database study and a questionnaire study among patients with T2DM. Results: In the database study, 2669 patients with T2DM and 2669 non-diabetes patients were included. MSK disorders were observed in 16.3% of patients with T2DM compared to 11.2% of non-diabetes patients (p < 0.001, OR 1.53, 95% CI 1.31, 1.80). In the questionnaire study, 200 patients with T2DM were included who reported a lifetime prevalence of painful upper extremity body sites for at least four weeks of 67.3%. Conclusion: We found that upper extremity MSK disorders have a high prevalence in Dutch patients with T2DM presenting in general practice. The prevalence ranges from 16% based on GP registered disorders and complaints to 67% based on self-reported diagnosis and pain. Early detection and treatment of these disorders may play a role in preventing the development of chronic MSK disorders.
Analyzing the Formation of Gaseous Emissions during Aluminum Melting Process with Utilization of Oxygen-Enhanced Combustion
Authors: Dzurňák Róbert , Varga Augustín , Jablonský Gustáv , Variny Miroslav , Pástor Marcel , Lukáč Ladislav
Published: February 2021

Oxygen-enhanced combustion (OEC) is a useful method for improving the efficiency of thermal plants and for decreasing greenhouse gas (GHG) emissions. Basic and modified burner designs utilizing OEC in the aluminum melting process in a rotary tilting furnace were studied. A combined approach comprising experimental measurement and simulation modeling was adopted aimed at assessing GHG emissions production. Reduction of up to 60% fuel consumption of the total natural gas used in the laboratory-scale furnace was achieved. The optimal oxygen concentration in the oxidizer regarding the amount of total GHG emissions produced per charge expressed as CO2 equivalent was 35% vol. Its further increase led only to marginal fuel savings, while the nitrogen oxide emissions increased rapidly. Using the modified burner along with OEC led to around 10% lower CO2 emissions and around 15% lower total GHG emissions, compared to using a standard air/fuel burner. CFD simulations revealed the reasons for these observations: improved mixing patterns and more uniform temperature field. Modified burner application, moreover, enables furnace productivity to be increased by shortening the charge melting time by up to 16%. The presented findings demonstrate the feasibility of the proposed burner modification and highlight its better energy and environmental performance indicators, while indicating the optimal oxygen enrichment level in terms of GHG emissions for the OEC technology applied to aluminum melting.
Legionellosis Caused by Non-Legionella pneumophila Species, with a Focus on Legionella longbeachae
Authors: Chambers Stephen T. , Slow Sandy , Scott-Thomas Amy , Murdoch David R.
Published: February 2021

Although known as causes of community-acquired pneumonia and Pontiac fever, the global burden of infection caused by Legionella species other than Legionella pneumophila is under-recognised. Non-L. pneumophila legionellae have a worldwide distribution, although common testing strategies for legionellosis favour detection of L. pneumophila over other Legionella species, leading to an inherent diagnostic bias and under-detection of cases. When systematically tested for in Australia and New Zealand, L. longbeachae was shown to be a leading cause of community-acquired pneumonia. Exposure to potting soils and compost is a particular risk for infection from L. longbeachae, and L. longbeachae may be better adapted to soil and composting plant material than other Legionella species. It is possible that the high rate of L. longbeachae reported in Australia and New Zealand is related to the composition of commercial potting soils which, unlike European products, contain pine bark and sawdust. Genetic studies have demonstrated that the Legionella genomes are highly plastic, with areas of the chromosome showing high levels of recombination as well as horizontal gene transfer both within and between species via plasmids. This, combined with various secretion systems and extensive effector repertoires that enable the bacterium to hijack host cell functions and resources, is instrumental in shaping its pathogenesis, survival and growth. Prevention of legionellosis is hampered by surveillance systems that are compromised by ascertainment bias, which limits commitment to an effective public health response. Current prevention strategies in Australia and New Zealand are directed at individual gardeners who use potting soils and compost. This consists of advice to avoid aerosols generated by the use of potting soils and use masks and gloves, but there is little evidence that this is effective. There is a need to better understand the epidemiology of L. longbeachae and other Legionella species in order to develop effective treatment and preventative strategies globally.
Nutraceutical Curcumin with Promising Protection against Herpesvirus Infections and Their Associated Inflammation: Mechanisms and Pathways
Authors: Šudomová Miroslava , Hassan Sherif T. S.
Published: February 2021

Herpesviruses are DNA viruses that infect humans and animals with the ability to induce latent and lytic infections in their hosts, causing critical health complications. The enrolment of nutraceutical anti-herpesvirus drugs in clinical investigations with promising levels of reduced resistance, free or minimal cellular toxicity, and diverse mechanisms of action might be an effective way to defeat challenges that hurdle the progress of anti-herpesvirus drug development, including the problems with drug resistance and recurrent infections. Therefore, in this review, we aim to hunt down all investigations that feature the curative properties of curcumin, a principal bioactive phenolic compound of the spice turmeric, in regard to various human and animal herpesvirus infections and inflammation connected with these diseases. Curcumin was explored with potent antiherpetic actions against herpes simplex virus type 1 and type 2, human cytomegalovirus, Kaposi’s sarcoma-associated herpesvirus, Epstein–Barr virus, bovine herpesvirus 1, and pseudorabies virus. The mechanisms and pathways by which curcumin inhibits anti-herpesvirus activities by targeting multiple steps in herpesvirus life/infectious cycle are emphasized. Improved strategies to overcome bioavailability challenges that limit its use in clinical practice, along with approaches and new directions to enhance the anti-herpesvirus efficacy of this compound, are also reviewed. According to the reviewed studies, this paper presents curcumin as a promising natural drug for the prevention and treatment of herpesvirus infections and their associated inflammatory diseases.