bluuebunny/crossref_metadata_2025 huggingface dataset:
Biological mechanisms of resistance to trastuzumab and ways to overcome them: Mod-ern problems of clinical oncology
Authors: Vynnychenko O. , Moskalenko R.
Published: August 2024

In 2022, 2.3 million new cases of breast cancer were registered in the world, which accounted for 11.6% of the total number of malignant neoplasms. Depending on the tumor's molecular profile, the prognosis for patients can be different. One of the most aggressive types is HER2-positive breast cancer. Trastuzumab, a recombinant humanized monoclonal antibody against HER2, is used to treat such tumors. Congenital or acquired resistance to trastuzumab is one of the essential problems in clinical oncology. Our study aimed to investigate the resistance mechanisms to trastuzumab and ways to overcome them. This drug influences several directions of oncogenesis at the same time. The fundamental mechanisms of action of trastuzumab are inhibition of HER2 ectodomain shedding, inhibition of angiogenesis, degradation of HER2 protein and its internalization, inhibition of DNA repair, influence on the phosphatidylinositol 3-kinase pathway, cell cycle and antibody-dependent cellular cytotoxicity. The biological mechanisms of resistance to trastuzumab are based on vascular mimicry and hypoxia, the appearance of breast cancer stem cells, activation of alternative signaling pathways, metabolic changes, alternative molecular variants of HER2, changes in the processes of immune regulation, heterogeneity of expression and stability of the HER2 protein. In modern clinical oncology, trastuzumab is used as an original product and as antibody-drug conjugates. Trastuzumab emtansine and trastuzumab deruxtecan are approved for the treatment of patients with HER2-positive breast cancer, including those with low HER2 expression. This literature review identified the biological resistance mechanisms to trastuzumab and ways to overcome them. The implementation of new targeted drugs in combination with trastuzumab is the way to personalized treatment. It can significantly improve the survival of patients with HER2-positive breast cancer.
Interpretation of the concepts of dentinal tubule and dentinal canaliculus
Authors: Popel S. L. , Gevkaliuk N. O. , Sydliaruk N. I. , Martyts Y. M. , Pynda M. Y. , Pudyak V. Y. , Krupey V. Y.
Published: August 2024

The biology of dentin occupies a central place in the theory of tooth integrity as an organ, and knowledge about the microstructure of dentin changes with the development of new research methods, such as scanning electron microscopy. The structures that form dentin, and the odontoblast processes, are an extremely complex biological structure due to cellular polarization. The structure of the dentin of crowns from 30 intact teeth extracted for orthodontic reasons has been studied. Dentin was examined on longitudinal chips using scanning electron microscopy, determining the average number of dentinal tubules per unit area of dentin, the length and diameter of the dentinal canaliculi along the dentinal tubule. Scanning electron microscopy of demineralized teeth showed that numerous fine fibrillar structures were found in the predentin, connecting the odontoblast processes (dentinal canaliculi) and the walls of the dentinal tubules. The odontoblast processes were always in close contact with the microfibrillar network and were located within the dentinal tubule. It was found that the dentinal canaliculi have their own anchoring microfibrillar system, which allows each dentinal canaliculus to be held in a central position. The microfibrils are attached to the wall of the dentinal tubule and form a continuous mesh structure among the dentin tissue and are directly fixed on one side to the wall of the dentinal tubule, and on the other side to the wall of the dentinal canaliculus, which is a derivative of the odontoblast process. In different areas of the dentin, the length, number, and diameter of the microfibrils have a wide variative range. Microstructures were numerous in different regions of the dentinal tubule. Their number decreased with distance from the level of the outer dentin. In the inner third of the dentinal tubule, microfibrillar structures are observed that form a dense network of different types, thicknesses and diameters. The most common direction of the microfibrils was from the surface of the odontoblast process to the wall of the dentinal tubule, and in other cases these microfibrils were attached to both opposite inner surfaces of the dentinal tubule. The base of the microfibril is attached to the wall of the dentinal tubule, as if forming a continuous structure among the dentin tissue and the surface of the odontoblast process. It is shown that the microfibrillar structures may be a previously unknown framework system that ensures the stabilization of odontoblast processes inside the dentinal tubule. In the scientific classification of dentin ultrastructure, it is advisable to use the term "dentinal tubule" and "dentinal canaliculus", since this structure is a derivative of the odontoblast process.
The translational content and expression of intracellular sensors under endoplasmic re-ticulum stress in diabetic rats
Authors: Klys Y. G. , Natrus L. V. , Kerimov T. R. , Smirnov S. M.
Published: August 2024

Endoplasmic reticulum stress (ER stress) activates the unfolded protein response (UPR) as a defense mechanism for maintaining cellular vital activity. Data on interaction between pathways of UPR-sensors, their role and participation in the recovery of regulatory proteins at constant level are currently being accumulated. An experimental model of type 2 diabetes mellitus (T2DM) was reproduced in male rats fed on 3-month high-fat diet with additional streptozotocin administration. Rats in different groups were treated with metformin, propionate, and their combination for 2 weeks. We analyzed the expression of the GRP78, PERK, IRE-1, and ATF6 genes in gastric glandulocytes using PCR primers. The quantitation of protein content of the GRP78, PERK, IRE-1, and ATF6 genes was performed using western blotting. Statistical intergroup differences were calculated using a one-way ANOVA test followed by Tukey’s post-hoc. Comparison of mRNA and protein contents at rest state, in condition of ER stress and drug treatment, as well as the study of correlations between UPR-sensors, provides a basis for the development of the concept of defense mechanism activation in gastric glandulocytes under T2DM conditions. Under stress, chaperone GRP78 plays a crucial role to prevent protein misfolding: it dissociates from ER, enters cisternae and inactivates misfolded proteins. The ATF6 pathway is activated in all conditions. It probably activates transcription of the corresponding content turnover sites in both GRP78 and ATF6. The IRE-1 and PERK pathways are activated in glandulocytes under different circumstances, and they probably target to different regulatory actions. Combination drug treatment in T2DM conditions induces activation of all signaling sensors in the UPR system with simultaneous maximal downregulation of GRP78, causing a balance in the intracellular homeostasis system.
Development of a mechanism for managing the level of investment in R&D of city-forming enterprises (by the example of the metallurgical industry)
Authors: Yessengaliyeva Saltanat , Tapalova Assel , Melekova Assel , Ibadildin Nurkhat , Kairliyeva Gaukhar , Serikbayev Serik , Nursapina Kanbibi
Published: August 2024

The object of the study is investment in R&D of enterprises. The problem of developing a mechanism for managing the level of R&D investment at industrial enterprises has been solved. The results are obtained:
– gradation of R&D investment levels at enterprises based on the application of the beta coefficient calculation methodology adapted to the R&D sphere;
– distribution of city-forming enterprises of the metallurgical industry by R&D investment levels: high level – 27 % of surveyed enterprises, average – 18 %, low – 55 %, critical – 0 %;
– development of a matrix based on the comparison of the level of R&D investment and the level of sales volumes of the enterprise's products calculated according to one algorithm. For each cell of the matrix, management decisions of strategic nature are proposed to increase the level of R&D investment.
The results obtained are explained by the assumption of a linear relationship between the size of R&D investment of a particular enterprise over time and aggregate industry R&D investment (as a benchmark). The tendency of R&D investment growth in the industry, regularity of R&D investments of the surveyed enterprises were confirmed, time series of R&D investment both at the evaluated enterprise and in the industry as a whole were processed using trend smoothing.
The features of the obtained results lie in the application of comparable coefficients (investments in R&D), as well as in the application of the developed assessment in the management of the enterprise.
The practical significance of this study results is the possibility of applying the obtained conclusions and recommendations to increase the level of R&D investment of large industrial enterprises in the volume, scope and conditions corresponding to the developing economy
Overcoming barriers to digitalization of small and medium-sized enterprises under martial law
Authors: Semeniuk Svitlana , Levytskyi Vitalii , Fomina Olena , Fedorchenko Kostiantyn , Yudina Nataliya , Ratynskiy Vadym , Shcherbatiuk Olena , Bendiuh Vladyslav , Zhurakivska Yuliia
Published: August 2024

The object of this study was the digitalization of small and medium-sized enterprises under martial law. The problem being solved was to overcome obstacles that slow down the introduction of digital technologies into the business processes of small and medium-sized enterprises during periods of conflict or war. During the period of martial law, the consequences for small and medium-sized enterprises are instability, material losses, resource limitations, and security threats. Despite the reduction in the number of small and medium-sized enterprises in Ukraine during the military invasion by 29.39 %, most of them continued to function, adapting to the new conditions of the crisis situation. However, neither the crisis conditions nor the increase in Internet users prompted these enterprises to make significant changes in the use of digital technologies. This was due to a number of barriers, including the following. The lack of liquidity and financing, the forced nature of implementation, the need to constantly adapt business processes to external changes are more related to the crisis situation. Inappropriate digital tools, lack of package solutions, lack of sufficient knowledge and skills, low trust in the state, small size of the enterprise, lack of understanding of the need or direction of reformatting business processes do not depend on the state of war. Overcoming the barriers to digitization of small and medium-sized enterprises under martial law is envisaged through interaction and partnership with other stakeholders. Cooperation and partnership are based on financial and informational support, training, platform model, and shared use of resources. The necessity and possibility of applying the developed proposals to overcome the barriers of digitalization of small and medium-sized enterprises under the conditions of martial law predetermines the practical significance of reported results
Features of lipid metabolism in patients with type 2 diabetes mellitus and gout
Authors: Stoianova Yuliia
Published: August 2024

The aim: To study the relationship between lipid metabolism parameters, chemerin, and adenosine monophosphate-activated protein kinase (AMPK) activity in patients with type 2 diabetes mellitus (T2DM), as well as in patients with a combined course of T2DM and gout.
Materials and methods. To assess lipid metabolism disorders, 100 patients were examined and divided into 2 groups: - 1st group – patients with T2DM and gout (n=70), 2nd group – patients with gout (n=30), control group (CG) - practically healthy individuals (n=20). The levels of total cholesterol (TC), triglycerides, high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), and very low-density lipoprotein cholesterol (VLDL) were analyzed. As markers of insulin resistance (IR), the content of AMPK and chemerin was investigated. Statistical data processing was carried out using the statistical data processing program package version 8.0 STATISTICA (StatSoft Inc).
The results. A significant difference was found between the levels of lipid parameters (except HDL) when comparing patients with the combined pathology of T2DM and gout, with a monocourse of gout and representatives of CG (р<0.001). It was also found that among lipidogram indicators, in all groups, an inverse correlation of AMPK value with LDL level was found (strong for patients of the 1st group and medium strength for representatives of the 2nd group and CG). Another indicator with which AMPK levels in all groups were statistically reliably correlated was TC. The presence of a direct correlation between the levels of LDL and chemerin in the 2nd group and CG, as well as the levels of TC and chemerin in patients with a combination of T2DM with gout and an inverse correlation in the other groups (all p<0.05) was established.
Conclusions: Statistically significant relationships were found between lipid metabolism indicators, chemerin and AMPK activity in patients with T2DM, as well as in patients with combined course of T2DM and gout
Features of eating behavior of early school-age children in the context of the implementation of the school meals reform
Authors: Petrenko Olena , Gulich Maria
Published: August 2024

The aim of this study is to study the peculiarities of the eating behaviour of children of primary school age, their food preferences, choice of products and attitudes towards school meals, food habits in the choice of products and attitudes towards the school canteen in the context of the implementation of the school meal reform.
Materials and methods. A large-scale medical and sociological study was conducted, which included children (5,401) of primary school age (from 6 to 10 years old) and their parents (4,347 people) from various schools in Kyiv, Vinnytsia, and Donetsk regions. The questionnaires included questions about food preferences, frequency of consumption of different foods, attitudes towards school meals and factors that influence food choices. The collected data were processed and analyzed using descriptive statistics methods in Excel.
Research results indicate a significant level of awareness of the importance of a healthy diet by most children, but an insufficient level of water consumption, an insufficient understanding of the importance of nutrition for maintaining health by children, high popularity of high-carbohydrate products and a low level of satisfaction of some parents with school meals.
Conclusions. The conducted analytical research revealed several educational and organizational problems related to the introduction of healthy food in schools, especially within the framework of reforming school food programs. According to the Strategy for reforming the school nutrition system, compliance with the principles of healthy nutrition, ensuring high-quality, safe and healthy nutrition, and improving the taste properties of school nutrition compliance with the principles of healthy nutrition, is extremely important for the preservation of children's health and the prevention of chronic non-infectious diseases. The results of our research indicate the need for additional educational programs for children and parents, the gradual introduction of new food standards, as well as ensuring the availability of healthy food in school canteens, and the implementation of social projects that will contribute to the formation of healthy eating habits
Analysis of methods and algorithms for processing unstructured text data based on JSON technology
Authors: Kucherenko Yehor , Kulakovska Inessa
Published: August 2024

The object of research is the process of automating systems for structuring data from several sources. The subject of the research is methods and algorithms for implementing a complete system for automated and parallel processing, validation and structuring of data. One of the most problematic areas is the merging of databases with different structures and several common fields into a generalized structure. The research was aimed at developing a system to increase the efficiency of automation of big data processing.
As a result of the work, optimization methods were studied, the influence of their internal parameters on the operation of algorithms was analyzed, their main advantages and disadvantages were determined, and software was developed in which the corresponding methods were implemented. An algorithm for structuring data before processing has been obtained. Data structuring is achieved by performing the «mapping» operation. Mapping can take place by indexes of already cleaned data or using a defined dictionary with a given set of keys, which allows not to care about the sequence of storing values and their possible shift.
The practical significance of the developed system lies in the improvement of methods of collecting and processing information for the purpose of its further validation, cleaning and accumulation in the following categories: geographic addresses and geo-coordinates, validation and automated addition of a mobile phone number to the international format, processing of car numbers (in modern and outdated format), VIN code of the engine and car brand, validation of urls of social networks, passport data and processing of personal data. Compared to similar methods for processing large volumes of data, the possibility of splitting the input file or stream into separate parts was used, the cleaned data from which is combined at the end of the system operation. Thanks to this, it is possible to process data whose size exceeds the available volume of the device's RAM, and the method of working with loosely structured text files in CSV format has been improved.
Modeling relationships in non-commutative two-operand two-bit cet-operations of a double cycle when permuting the operands
Authors: Rudnytskyi Volodymyr , Lada Nataliia , Herashchenko Maksym , Korotkyi Tymofii , Stabetska Tetiana
Published: August 2024

The object of the research is relationships in non-commutative two-operand two-bit CET-operations of a double cycle when operands are permuted. The article is devoted to studying the results of the computational experiment, which is in building a model of relationships in non-commutative two-operand two-bit CET-operations of a double cycle with the operands permutation in order to ensure the possibility of building cryptographic systems with XOR sequence encryption. The theoretical and practical results of the work are obtained on the basis of the computational experiment data. The results of researching the CET-operations data make it possible to build cryptographic systems with XOR sequence ciphering and to improve the quality of low-resource stream encryption systems. The mathematical description of the computational experiment results made it possible to establish relationships between pairs of non-commutative two-operand two-bit CET-operations of a double cycle when operands are permuted. The possibility of constructing a group of commutative two-operand two-bit CET-operations of a double cycle based on the modification of a known two-operand operation by one-operand operations to within the permutation of the crypto-transformation results has been studied. The correctness of constructing a group of CET-operations, both without operand permutation and such that allow operand permutation, has been verified. The model for building a group of asymmetric two-operand two-bit CET-operations of a double cycle, which allow the operands permutation is proposed. Applying the substitution model made it possible to obtain pairs of interrelated operations in this group. The obtained pairs of interconnected operations provide a description of modification for direct and inverse non-commutative CET operations when permuting the operands. The obtained results provide the possibility of building cryptographic systems that encrypt both the input open information under the control of the XOR sequence and the XOR sequence under the control of the input open information. Further research will be aimed at establishing relationships in non-commutative two-operand two-bit CET operations of the triple cycle when operands are permuted.
Experience and effectiveness of the no-loft linking shape and dimension method using laser optical systems in aircraft production
Authors: Lupkin Borys , Maiorova Kateryna , Pavlenko Oleksiy , Tolstoy Sergey , Voronko Iryna
Published: August 2024

The object of research is the application of the no-loft linking shapes and dimension method using laser measuring tools to reduce the labor intensity and cycle of mounting work. The study of the accuracy of the geometric parameters of cantilever wing and technological equipment at various stages of production was carried out. The problem is to create a method of using laser optical systems in aircraft production at the stage of mounting of technological equipment to minimize the impact on the accuracy of the dimensions of assembled parts of aggregates. The following results were obtained: the advantages of using the no-loft linking method in the modern production of aviation equipment were analyzed, which makes it possible to reduce the preparation cycle by 2–3 times. A study was conducted on the effective use of the Coordinate Measuring Machine (CMM) of laser tracker in the manufacture of the cantilever wing (CW) of the AN series airplane at all stages of mounting, as well as on the accuracy inspection of geometric parameters in comparison with the theoretical master geometry (MG).
The practical significance of the research is that the proposed method of using laser optical systems during the installation of equipment allows to reduce to a minimum the impact on the accuracy of low rigidity frames. And also, to reduce the equipment deformation due to the mass of the parts of the assembled aggregates and temperature deformations, which allows to ensure a reduction of the mounting error to ±0.1 mm. Also, the application of this technique allows to enter the plane's coordinate system without prior leveling, to mounting and inspection the installation of the wing, fin, stabilizer, engines and landing gear on the fuselage. In general, the application of the no-loft linking shape and dimension method with using laser optical systems in aircraft production allows to reduce the labor intensity and cycle of mounting work up to 10 times.
Determination of the characteristics of drill string vibrations during the drilling process
Authors: Titlov Oleksandr , Svitlytskyi Viktor , Iagodovskyi Sergii
Published: August 2024

The object of research is vibration processes of a certain origin in the drill string with typical design deviations depending on the mode parameters of drilling. A drill string is an oscillating system with an infinite number of degrees of freedom of a multifactor system. An exhaustive study of oscillatory processes in the drill string is impossible neither analytically nor experimentally, due to the specifics of the hole deepening in various rocks, the design of the well, its shape, etc. Therefore, in practice, they try to solve the problems of the dynamics of the drill string for an idealized system and, while preserving the main oscillatory properties, solve some problems of the rod system. The work carried out was aimed at experimental studies of vibrations of the drill string during the drilling process.
It is shown that the effectiveness of the use of hydrodynamic cavitation requires the development of methods and devices for intensifying the well drilling process. It is proven that the design of the cavitation generator organically fits into the existing well drilling equipment and allows for the intensification of technological processes with lower specific energy consumption. It is found that all oscillatory processes that occur in the drill string are random in nature and must be considered using the mathematical apparatus of the theory of random oscillations.
The study of vibrations during well drilling shows that vibrations can be considered as random stationary processes, since transient modes have a sufficiently short duration for homogeneous rocks with fixed drilling modes. The analysis of the vibrations of the drill string elements based on random oscillations in a number of cases allows to increase the reliability of determining the vibration reliability of the drill string elements. It has been proven that the response of drill string elements to broadband random vibration can be defined as the combined effect of several narrowband random vibrations.
Application of Genetic Algorithm for Synthesizing Onedimensional Thinned Antenna Arrays to form Twodimensional Thinned MIMO Antenna Arrays
Authors:   Novosibirsk State Technical University, Sokolov V. S. , Stepanov M. A. ,   Novosibirsk State Technical University, Artyushenko V. V. ,   Novosibirsk State Technical University, Podkopaev A. O. ,   Novosibirsk State Technical University
Published: August 2024

The possibility of using thinned classical linear antenna arrays to synthesize two-dimensional thinned MIMO antenna arrays on their basis is considered. The MIMO antenna array which receiving and transmitting elements are located on two mutually orthogonal lines is regarded. On the example of this array it is shown that the directivity diagram of its virtual aperture has the property of separability of spatial variables. It allows using the arrangement of elements of a thinned classical linear antenna array for the receiving and transmitting elements lines. It is demonstrated that a thinned MIMO antenna array can form a radiation pattern with a lower level of side lobes than a full array while keeping the width of the main lobe unchanged. The dependence allowing to determine the peak level of side lobes of a twodimensional thinned MIMO antenna array at a given thinning factor is given.
ESPORTE E VALORES: CONTRIBUIÇÕES PARA A FORMAÇÃO DA PERSONALIDADE MORAL
Authors: Hirama Leopoldo Katsuki , Joaquim Cássia dos Santos , Galatti Larissa Rafaela , Montagner Paulo César
Published: August 2024

O esporte tem sido proclamado como excelente “ferramenta” para desenvolver valores para a boa convivência. No entanto, o número reduzido de estudos sobre este tema, sobretudo a respeito de propostas de intervenção, indicam que esta relação pode estar mais no campo da especulação que no da pedagogia baseada em fundamentos científicos. O objetivo dessa pesquisa foi analisar o processo de estimulação da construção da personalidade moral no ambiente de ensino do esporte a partir de um estudo de caso, orientado pela abordagem etnográfica com observação participante. Investigamos propostas de intervenção no ambiente de ensino do judô para a formação da personalidade moral de crianças e jovens durante cinco anos de um projeto extensionista.  Como resultado apresentamos a discussão de quatro categorias temáticas: i- A comunidade e seus valores; ii- Heteronomia como passagem para a autonomia moral; iii- Princípios adotados no ambiente do ensino do esporte ; iv-  A profundidade no aprendizado como fundamento do desenvolvimento moral. Concluímos que a contribuição na formação da personalidade moral é possível, que o ambiente do ensino do esporte pode representar  espaço de relações significativas com este foco, mas exige intencionalidade e a criação de cultura moral rica.
Funções parentais desempenhadas por madrastas e a coparentalidade do novo casal
Authors: Xavier Scremin Ana Luiza , Fragoso Cousseau Bruna , Reck Brum Rayssa , Rubin Rossato Pereira Caroline
Published: August 2024

Considera-se a família como uma instituição em constante transformação, tanto em sua configuração como nos papéis exercidos por seus membros. As funções parentais não são exclusivas dos pais, mas podem ser desempenhadas por qualquer pessoa que deseja assumir esse papel. Considerando o recasamento, então se torna interessante pensar como acontece essa coparentalidade nos casos onde apenas um membro do casal possua filhos. O estudo objetivou compreender as funções parentais exercidas por madrastas junto a seus enteados, no contexto de famílias recasadas. É um estudo de caso coletivo, de cunho qualitativo e exploratório.  Participaram três famílias, compostas por pai, madrasta e pelo menos um filho, que residiam juntos há no mínimo seis meses. Os participantes responderam a um questionário de dados sociodemográficos e a uma entrevista semi-estruturada, intitulada Entrevista sobre a parentalidade na família recasada. Identificou-se que as madrastas desempenhavam um papel importante na vida dos enteados em termos de funções parentais, assumindo responsabilidades parentais em uma relação de apoio e afeto.
Construção da identidade paterna e licença paternidade: uma perspectiva de pais
Authors: Do Couto Bueno Julia , Aparecida Serralha Conceição
Published: August 2024

O objetivo deste estudo é analisar a perspectiva paterna acerca da licença paternidade e a utilidade desta para a construção da identidade paterna. Participaram desta pesquisa 10 pais-homens: cinco, que tiveram licença paternidade de cinco dias, e outros cinco, que tiveram licença paternidade de 20 dias. Os participantes preencheram um formulário sociodemográfico e realizaram uma entrevista semiestruturada individual por meio de vídeo chamada, que foi gravada e transcrita. Os dados foram analisados através da análise temática e os resultados foram discutidos com subsídios da teoria psicanalítica winnicottiana. Os resultados mostraram que a licença paternidade é um período valorizado pelos homens e que incentiva a paternidade, além de exercer influência na dupla mãe-bebê. Porém, notou-se que a forma como a licença paternidade é executada não contempla as necessidades dos pais-homens, das mães e dos filhos, pois a volta breve ao trabalho se torna um impeditivo para o exercício das funções demandadas. Logo, é necessário o desenvolvimento de espaços de escuta e acolhimento para que esses homens possam expor os seus anseios e angústias acerca da paternidade. Esses espaços também são importantes para se entender ainda mais o quão efetivo é esse período de licença.
Experiência de supervisão de estágio específico em avaliação psicológica
Authors: Schütz Adriana , Balem Yates Denise , Neves Portugal Paula
Published: August 2024

Como uma especialidade da psicologia, a avaliação psicológica (AP) constitui uma das práticas de ensino de muitas instituições de formação de nível superior. No entanto, há uma escassez de publicações e regulamentações acerca da supervisão em AP, que integra características básicas de supervisão em psicologia clínica com aspectos de ensino de conteúdos específicos. Além disso, no Brasil, poucos serviços oferecem prática de estágio exclusiva em AP. O Centro de Avaliação Psicológica (CAP) da UFRGS é um serviço-escola que mantém estágio profissional em AP há mais de 20 anos. Em função desta larga experiência, o presente artigo teve como objetivo relatar práticas de supervisão desenvolvidas no CAP através de uma abordagem desenvolvimental e com foco nos preceitos da prática de avaliação psicológica no Brasil a partir da experiência de três supervisoras entre os anos de 2021 e 2023. As observações foram discutidas, categorizadas e descritas a partir de três temas: (1) funções específicas das supervisoras na supervisão em AP, (2) funcionamento das supervisões e (3) percepção das experiências de supervisão durante a pandemia. Através dos pontos discutidos, denotou-se a importância da formação e qualificação do supervisor para a adequada condução da supervisão, além das vantagens da adoção de uma abordagem focada no desenvolvimento e nas competências do aluno. Foram discutidos também desafios da supervisão remota e sua eficácia para o processo de aprendizagem de alunos de graduação, com foco em direcionamentos para aplicações futuras.
Prevalencia de prácticas cuestionables de investigación en la sociología española: aproximación comparada a otras disciplinas y países
Authors: De Gracia Palomera Daniel , Suárez-Vergne Álvaro
Published: August 2024

El objetivo del presente artículo es explorar la prevalencia de prácticas cuestionables de investigación (en adelante, PCI) de la sociología en España desde una perspectiva comparada con otras disciplinas y países del entorno. Para ello se hace uso de la International Research Integrity Survey (IRIS) (Allum y Reid, 2022), una base de datos realizada a partir de un cuestionario a investigadores e investigadoras de multitud de disciplinas y países. Tras un análisis estadístico de comparación de proporciones por el método Bonferroni, se destaca que, en España, la Sociología muestra una alta prevalencia en dos PCI específicas: no citar trabajos contrarios a las propias creencias y realizar investigaciones sin aprobación ética. A nivel interdisciplinar, estas prácticas son más prevalentes en sociología que en ciencias naturales e ingeniería, y a nivel internacional, más que en Italia, Alemania y el Reino Unido. La investigación sugiere que estas diferencias no se deben a la propia disciplina, sino a los diferentes contextos nacionales o institucionales.
Cas9 Protein Purification Protocol v1
Authors: Huang Shu
Published: August 2024

This protocol outlines the steps for purifying Cas9 protein, essential for the preparation of RNP complexes used in CRISPR-Cas9 editing processes.
OT-2 Custom Mixing with Single or Multi-Channel Pipette v1
Authors: Goñi-Moreno Ángel , Mariya Anhel Ana , Mugica-Galan Paula
Published: August 2024

This protocol is created to help efficiently produce high-throughput plates with different reagents using either a single or multi-channel pipette controlled by an Opentrons OT-2 robot. By running this script, users can achieve a precise layout of reagents in specified volumes across the final plate(s) using a single-channel or multi-channel pipette based on a customizable Excel input file. The protocol uses a Python script for the Opentrons OT-2 robot in conjunction with an Excel file that contains all the necessary parameters and will be processed by the Python script. This Excel file contains customizable variables such as the number of final plates, transfer volumes, plate types, and the type of pipette used. This method ensures the accurate distribution of reagents based on user-defined configurations, making the high-throughput process more efficient. This protocol has been used in our laboratory to check how different quorum sensing systems work in P. putida KT2440. This protocol is a set of instructions or description of the LAP repository entry LAP-CustomReagentMixingMultiSinglePip-OT2-1.0.0 You can find the script and complementary information for this specific version of the protocol in this LAP entry link and GitHub Link to LAP entry documents
Competent Transformation v1
Authors: Estévez-Gay Miquel
Published: August 2024

Transform a Plasmid into previously obtained Competent E.coli Cells.
Tonsil Collection and Processing Protocol v1
Authors: H. Randell Scott
Published: August 2024

For preserving human tonsils by formalin fixation, O.C.T. embedding and freezing, Cryostor freezing medium, and snap freezing in liquid nitrogen.
Obtaining Competent Cells v1
Authors: Estévez-Gay Miquel
Published: August 2024

Obtencion of 100ml competent E.coli aliquots that must be stored in -80C
ERRATUM (An erratum has been prepared for the following articles in the 33rd volume and 2nd issue of Current Research in Dental Sciences in 2023.)
Authors: E. Erratum
Published: August 2024

Erratum

An erratum has been prepared for the following articles in the 33rd volume and 2nd issue of Current Research in Dental
Sciences in 2023. - In the article titled "Özyurt E, Kurt A. Effect of Different Teas on Surface Roughness of Conventional and Bulk-Fill Composite
Resins Beverage Effect on Composite Resins. Curr Res Dent Sci. 2023; 33(2): 84-89", a patient consent draft was added
inadvertently, even though there was no participant. - In the article titled "Keleş ZH, Ozan G. Effect of preheating on the microhard- ness and depth of cure of two composite resins
with and without BIS-GMA. Curr Res Dent Sci. 2023; 33(2): 79-83", a patient consent draft was added inadvertently, even
though there was no participant. - In the article titled “Yamalı AY, Turhan Bal B. Properties and clinical applications of lithium disilicate ceramics. Curr Res Dent
Sci. 2023; 33(2): 122-130” although the article titled is a compilation, a patient consent draft was mistakenly included. - In the article titled "Eriş Derkuş F, Yılmaz UN. Demographic Analysis, Diagnosis, and Treatment Methods of Maxillofacial Trauma Cases in the Southeastern Anatolia Region of Turkey: Retrospective and Comparative Analysis. Curr Res Dent Sci.
2023; 33(2): 103-107", it was forgotten to write Dicle University in the sections left as XXX in the Methods section for the
sake of article blindness. - Since the study titled “Yıldızer Keriş E. Comparison of Articular Eminence Steepness in Patients With Temporomandibular
Joint Disc Disorders. Curr Res Dent Sci. 2023; 33(2): 117-121” was retrospective, patient consent could not be obtained and
this information was forgotten to be added. - In the article titled “Şener E, Baksı Şen G. 3D Modelling and virtual planning technologies in dentistry. Curr Res Dent Sci.
2023; 33(2): 131-134” although the article titled is a compilation, a patient consent draft was mistakenly included.
Physical Comorbidity and Healthcare Utilization in People with Schizophrenia: a systematic review
Authors: Salvador Mercè , Porras-Segovia Alejandro , Lopez-Fernandez Olatz , Baca-Garcia Enrique
Published: August 2024

Introduction. Schizophrenia is a mental health condition that can lead to significant disability and have a major impact on social and occupational functioning. It tends to decrease life expectancy, which could be attributed to a variety of factors, including poor health habits, barriers to healthcare access, and the stigmatization of mental illness. Objective. The main aim of this systematic review is to examine the physical comorbidity of these patients, and their health service utilization. Method. The protocol has been registered with the PROSPERO database (CRD42020139972) and follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A search of PubMed/MEDLINE, EMBASE, Scopus, Web of Science, PsycINFO, Cochrane Library, and ProQuest Health Research Premium Collection yielded 317 articles, 57 of which were selected. Results. The mortality rate of people with schizophrenia is often at least twice that of the general population. In addition, these individuals may be susceptible to other physical health conditions that can significantly shorten their lifespan: cardiovascular and respiratory diseases, diabetes mellitus, oncologic diseases, and chronic infections. Complications during hospitalization and prolonged length of stay due to comorbidities may impede their access to essential healthcare. Discussion and conclusion. Enhancing the healthcare system is crucial to providing adequate care for these patients. Adopting positive lifestyle choices, reducing substance dependence, and abstaining from unconventional antipsychotics can bolster their life expectancy. It is therefore crucial to implement intervention strategies while training professionals to detect and manage physical comorbidities associated with this disorder.
Unleashing the future: Exploring the transformative prospects of artificial intelligence in veterinary science
Authors: Sharun Khan , Banu S. Amitha , Mamachan Merlin , Abualigah Laith , Pawde A. M. , Dhama Kuldeep
Published: August 2024

Artificial intelligence (AI) has emerged as a transformative paradigm, promising revolutionary advancements in animal healthcare. Leveraging AI's unparalleled capacity for rapid data analysis significantly enhances diagnostic precision and speed, thereby facilitating informed decision-making by veterinarians. Predictive medicine powered by AI not only anticipates disease outbreaks but also enables tracking zoonotic diseases and predicting individual health risks for animals. AI helps to generate personalized treatment plans by analyzing genetic, environmental, and historical data. Remote monitoring and telemedicine, empowered by AI, overcome geographical constraints and offer continuous care, enabling veterinarians to track vital signs and intervene promptly. However, as AI becomes integral to veterinary practice, ethical considerations surrounding data privacy, transparency, and responsible AI use are crucial. This review explores the scope of AI in enhancing research and drug development, highlighting its ability to improve the discovery process and contribute to novel therapeutic interventions. It emphasizes the necessity of maintaining a delicate balance between AI-driven automation and the expertise of veterinary professionals. As the veterinary community moves toward embracing the transformative potential of AI, this comprehensive examination provides valuable insights into the current scenario. It discusses the challenges, opportunities, implications, and ethical considerations that shape the future of AI in veterinary science.
Hematite Nanoparticle Mediated Enhancement of Chlorella minutissima Lipid Productivity for Sustainable Biodiesel Production
Authors: Pahariya Richa , Chauhan Abhishek , Ranjan Anuj , Basniwal Rupesh Kumar , Upadhyay Sumant , Kataria Smile , Tuli Hardeep Singh , Shahwan Moyad , Pathak Vinay Mohan , Jindal Tanu
Published: August 2024

This study aims to enhance lipid and biofuel productivity from Chlorella minutissima with hematite (α-Fe2O3) nanoparticles (IONPs) as a growth stimulant. The IONPs were synthesized using chemical method and characterized using X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX) analysis to confirm their structure and composition. The experimental setup involved inoculating various concentrations of IONPs (10, 20, and 30 mg·L−1) into the microalgal BG-11 growth medium to evaluate their impact on microalgal growth and biodiesel production. Results of this study showed that a concentration of 10 mg·L−1 of IONPs significantly increased the biomass concentration to 508.1 mg·L−1 over a 20-day cultivation period, achieving the highest biomass production rate of 31.7 mg·L−1·d−1 at this concentration. The lipid extracted from the microalgal biomass was subsequently transesterified into biodiesel. Key biodiesel properties, such as cetane number, calorific value, density, and viscosity, were measured to assess fuel quality. The findings demonstrate that incorporating hematite nanoparticles into the microalgal growth medium can significantly boost both lipid content and overall growth, thereby improving biodiesel production. This study suggests that the use of α-Fe2O3 nanoparticles presents a promising approach for scalable and sustainable biofuel production from microalgae.
Germination of Senegalia mellifera seeds in response to presowing treatments
Authors: Madisa Fiona Opelo , Mojeremane Witness , Makgobota Kamogelo , Teketay Demel , Isaac Makoi Topoyame
Published: August 2024

This study aimed to evaluate the size of Senegalia mellifera seeds and determine the most effective scarification techniques to improve germination. The experiment, conducted at Botswana University of Agriculture and Natural Resources, involved ten presowing treatments, including control, nicking, soaking in sulphuric acid for different durations, and boiling water for varying periods. A completely randomized design (CRD) was used for the experiment. Germination data was transformed using arcsine to meet normal distribution requirements and then analyzed using analysis of variance (ANOVA). The results showed that the presowing treatments had a statistically significant effect on germination (P<0.00001). Seeds treated with sulphuric acid, nicking, and those left untreated exhibited the highest germination rates, while seeds treated with boiling water showed the lowest germination percentages. These findings indicate that the seed coats of S. mellifera are permeable to water and air, and presowing treatments do not show any significant effect on the successful germination of S. mellifera seed.
Effect of Preparation and Drying Techniques on the Physicochemical, Functional and Nutritional Properties of products from Beetroot (Beta Vulgaris L.) varieties
Authors: Mubajje M. S. , Lubowa M. , Yeoh S.Y. , Acham H. , Tumuhimbise G. A. , Matovu M.
Published: August 2024

Beetroot (Beta vulgaris L.) is rich in biologically active compounds. This study aimed to assess how different methods of preparation and drying affect the physical, chemical, functional, and nutritional properties of iron-rich beetroot powder. Two beetroot varieties, Detroit Dark Red (DetR) and Crimson Globe (CrimG), were processed using three drying techniques: sun drying (SD), oven drying (OD), and freeze drying (FD), with both boiled and fresh beetroots. The properties evaluated in the study included water activity, color, total phenolics and flavonoids, oxalate content, and mineral content. The results showed significant (p<0.05) differences in these properties between the dried and fresh samples. Notably, drying increased calcium, zinc, and phosphorus levels while decreasing the iron content. Boiling followed by sun drying was the best method for retaining iron, particularly for the CrimG variety. The study suggests that drying can help preserve or even enhance the physicochemical properties and micronutrient content, especially iron while reducing phytochemical levels affecting iron absorption. These findings are important for developing iron-rich beetroot products to improve dietary iron intake, especially for adolescent children.
The Implementation of Socioeconomic Well-Being of Waqf and Properties in Muhammadiyah Regional Board Yogyakarta
Authors: Mahfudz Akhmad Affandi , Syamsuri Syamsuri , Zein Anisa Rosdiana , Roslan Isma Aulia , Zeinullayevn Nurmagambetova Azhar
Published: August 2024

Waqf is one of the financial instruments that can improve the socioeconomic status of Ummah. However, the number of waqf assets exceeds expectations due to a lack of proper management. The socioeconomic well-being of the Ummah is among the core principles of waqf management in the form of accountability and transparency. This study aims to unveil the implementation of the Socioeconomic well-being of Waqf Board Management, taking the case of the Muhammadiyah Waqf Council in Yogyakarta, Indonesia. This research employs descriptive analysis through structured interviews, passive participation observation, and documentation. The study found that the principles of socio-economics well-being have been implemented by the Waqf Board Management of Muhammadiyah Yogyakarta in the form of accountability, including compliance and Integrity, Administration, Programs, Practice, and Finance. The principle of transparency includes the level of openness in the delivery of public services, ease of understanding of the rules and procedures for providing services, and the availability of information on various fields of public administration. This paper implies the massive policy direction of Waqf Board Management among the Muhammadiyah Organization in Indonesia, which is the second largest Muslim organization in the country, to ameliorate the way of implementing the socio-economics of waqf management.