bluuebunny/crossref_metadata_2025 huggingface dataset:
Microencapsulation of Bioactive Ingredients for Their Delivery into Fermented Milk Products: A Review
Authors: Gruskiene Ruta , Bockuviene Alma , Sereikaite Jolanta
Published: July 2021

The popularity and consumption of fermented milk products are growing. On the other hand, consumers are interested in health-promoting and functional foods. Fermented milk products are an excellent matrix for the incorporation of bioactive ingredients, making them functional foods. To overcome the instability or low solubility of many bioactive ingredients under various environmental conditions, the encapsulation approach was developed. This review analyzes the fortification of three fermented milk products, i.e., yogurt, cheese, and kefir with bioactive ingredients. The encapsulation methods and techniques alongside the encapsulant materials for carotenoids, phenolic compounds, omega-3, probiotics, and other micronutrients are discussed. The effect of encapsulation on the properties of bioactive ingredients themselves and on textural and sensory properties of fermented milk products is also presented.
Novel Bifunctional [16]aneS4-Derived Chelators for Soft Radiometals
Authors: Straathof Natan J. W. , Magnus Charlotte B. , Zhuravlev Fedor , Jensen Andreas I.
Published: July 2021

The field of targeted radionuclide therapy is rapidly growing, highlighting the need for wider radionuclide availability. Soft Lewis acid ions, such as radioisotopes of platinum, rhodium and palladium, are particularly underdeveloped. This is due in part to a lack of compatible bifunctional chelators. These allow for the practical bioconjugation to targeting vectors, in turn enabling radiolabeling. The [16]andS4 macrocycle has been reported to chelate a number of relevant soft metal ions. In this work, we present a procedure for synthesizing [16]andS4 in 45% yield (five steps, 12% overall yield), together with a selection of strategies for preparing bifunctional derivatives. An ester-linked N-hydroxysuccimide ester (NHS, seven steps, 4% overall yield), an ether-linked isothiocyanate (NCS, eight steps, 5% overall yield) and an azide derivative were prepared. In addition, a new route to a carbon-carbon linked carboxylic acid functionalized derivative is presented. Finally, a general method for conjugating the NHS and NCS derivatives to a polar peptide (octreotide) is presented, by dissolution in water:acetonitrile (1:1), buffered to pH 9.4 using borate. The reported compounds will be readily applicable in radiopharmaceutical chemistry, by facilitating the labeling of a range of molecules, including peptides, with relevant soft radiometal ions.
Molecularly Imprinted Polymers Combined with Electrochemical Sensors for Food Contaminants Analysis
Authors: Elfadil Dounia , Lamaoui Abderrahman , Della Pelle Flavio , Amine Aziz , Compagnone Dario
Published: July 2021

Detection of relevant contaminants using screening approaches is a key issue to ensure food safety and respect for the regulatory limits established. Electrochemical sensors present several advantages such as rapidity; ease of use; possibility of on-site analysis and low cost. The lack of selectivity for electrochemical sensors working in complex samples as food may be overcome by coupling them with molecularly imprinted polymers (MIPs). MIPs are synthetic materials that mimic biological receptors and are produced by the polymerization of functional monomers in presence of a target analyte. This paper critically reviews and discusses the recent progress in MIP-based electrochemical sensors for food safety. A brief introduction on MIPs and electrochemical sensors is given; followed by a discussion of the recent achievements for various MIPs-based electrochemical sensors for food contaminants analysis. Both electropolymerization and chemical synthesis of MIP-based electrochemical sensing are discussed as well as the relevant applications of MIPs used in sample preparation and then coupled to electrochemical analysis. Future perspectives and challenges have been eventually given.
Non-Specific GH30_7 Endo-β-1,4-xylanase from Talaromyces leycettanus
Authors: Šuchová Katarína , Spodsberg Nikolaj , Mørkeberg Krogh Kristian B. R. , Biely Peter , Puchart Vladimír
Published: July 2021

This study describes the catalytic properties of a GH30_7 xylanase produced by the fungus Talaromyces leycettanus. The enzyme is an ando-β-1,4-xylanase, showing similar specific activity towards glucuronoxylan, arabinoxylan, and rhodymenan (linear β-1,3-β-1,4-xylan). The heteroxylans are hydrolyzed to a mixture of linear as well as branched β-1,4-xylooligosaccharides that are shorter than the products generated by GH10 and GH11 xylanases. In the rhodymenan hydrolyzate, the linear β-1,4-xylooligosaccharides are accompanied with a series of mixed linkage homologues. Initial hydrolysis of glucuronoxylan resembles the action of other GH30_7 and GH30_8 glucuronoxylanases, resulting in a series of aldouronic acids of a general formula MeGlcA2Xyln. Due to the significant non-specific endoxylanase activity of the enzyme, these acidic products are further attacked in the unbranched regions, finally yielding MeGlcA2Xyl2-3. The accommodation of a substituted xylosyl residue in the −2 subsite also applies in arabinoxylan depolymerization. Moreover, the xylose residue may be arabinosylated at both positions 2 and 3, without negatively affecting the main chain cleavage. The catalytic properties of the enzyme, particularly the great tolerance of the side-chain substituents, make the enzyme attractive for biotechnological applications. The enzyme is also another example of extraordinarily great catalytic diversity among eukaryotic GH30_7 xylanases.
Comparative Evaluation on Impacts of Fibronectin, Heparin–Chitosan, and Albumin Coating of Bacterial Nanocellulose Small-Diameter Vascular Grafts on Endothelialization In Vitro
Authors: Wacker Max , Riedel Jan , Walles Heike , Scherner Maximilian , Awad George , Varghese Sam , Schürlein Sebastian , Garke Bernd , Veluswamy Priya , Wippermann Jens , Hülsmann Jörn
Published: July 2021

In this study, we contrast the impacts of surface coating bacterial nanocellulose small-diameter vascular grafts (BNC-SDVGs) with human albumin, fibronectin, or heparin–chitosan upon endothelialization with human saphenous vein endothelial cells (VEC) or endothelial progenitor cells (EPC) in vitro. In one scenario, coated grafts were cut into 2D circular patches for static colonization of a defined inner surface area; in another scenario, they were mounted on a customized bioreactor and subsequently perfused for cell seeding. We evaluated the colonization by emerging metabolic activity and the preservation of endothelial functionality by water soluble tetrazolium salts (WST-1), acetylated low-density lipoprotein (AcLDL) uptake assays, and immune fluorescence staining. Uncoated BNC scaffolds served as controls. The fibronectin coating significantly promoted adhesion and growth of VECs and EPCs, while albumin only promoted adhesion of VECs, but here, the cells were functionally impaired as indicated by missing AcLDL uptake. The heparin–chitosan coating led to significantly improved adhesion of EPCs, but not VECs. In summary, both fibronectin and heparin–chitosan coatings could beneficially impact the endothelialization of BNC-SDVGs and might therefore represent promising approaches to help improve the longevity and reduce the thrombogenicity of BNC-SDVGs in the future.
Theoretical Investigation of Proton Diffusion in Dion–Jacobson Layered Perovskite RbBiNb2O7
Authors: Shi Jing , Han Chang , Niu Haibo , Zhu Youzhang , Yun Sining
Published: July 2021

Perovskite materials are considered to be promising electrolyte membrane candidates for electrochemical applications owing to their excellent proton- or oxide-ion-conducting properties. RbBiNb2O7 is a double-layered Dion–Jacobson perovskite oxide, with Pmc21 symmetry. In this study, the electronic structure and proton-diffusion properties of bulk RbBiNb2O7 were systematically investigated using first-principles calculations. The unique layered crystal structure of RbBiNb2O7 plays a crucial role in proton storage and proton conductivity. Different proton-diffusion steps in RbBiNb2O7 were considered, and the activation energies of the relevant diffusion steps were evaluated using the climbing image-nudged elastic band (CI-NEB) technique. The proton diffusion in RbBiNb2O7 presents a two-dimensional layered characteristic in the a-b plane, owing to its layered crystalline nature. According to the transition state calculations, our results show that the bulk RbBiNb2O7 exhibits good proton-transport behavior in the a-b plane, which is better than many perovskite oxides, such as CaTiO3, CaZrO3, and SrZrO3. The proton diffusion in the Rb–O and Nb–O layers is isolated by a higher energy barrier of 0.86 eV. The strong octahedral tilting in RbBiNb2O7 would promote proton transport. Our study reveals the microscopic mechanisms of proton conductivity in Dion–Jacobson structured RbBiNb2O7, and provides theoretical evidence for its potential application as an electrolyte in solid oxide fuel cells (SOFCs).
Fundamental Aspects and Comprehensive Review on Physical Properties of Chemically Grown Tin-Based Binary Sulfides
Authors: Gedi Sreedevi , Minnam Reddy Vasudeva Reddy , Kotte Tulasi Ramakrishna Reddy , Park Chinho , Kim Woo Kyoung
Published: July 2021

The rapid research progress in tin-based binary sulfides (SnxSy = o-SnS, c-SnS, SnS2, and Sn2S3) by the solution process has opened a new path not only for photovoltaics to generate clean energy at ultra-low costs but also for photocatalytic and thermoelectric applications. Fascinated by their prosperous developments, a fundamental understanding of the SnxSy thin film growth with respect to the deposition parameters is necessary to enhance the film quality and device performance. Therefore, the present review article initially delivers all-inclusive information such as structural characteristics, optical characteristics, and electrical characteristics of SnxSy. Next, an overview of the chemical bath deposition of SnxSy thin films and the influence of each deposition parameter on the growth and physical properties of SnxSy are interestingly outlined.
Spontaneous Polarization Calculations in Wurtzite II-Oxides, III-Nitrides, and SiC Polytypes through Net Dipole Moments and the Effects of Nanoscale Layering
Authors: Troy William , Dutta Mitra , Stroscio Michael
Published: July 2021

Herein, the spontaneous polarization in crystals with hexagonal symmetry are calculated as a function of the number of monolayers composing a nanostructure by adding the dipole moments for consecutive units of the nanostructure. It is shown that in the limit of a large numbers of monolayers that the spontaneous polarization saturates to the expected bulk value of the spontaneous polarization. These results are relevant to understanding the role of the built-in spontaneous polarizations in a variety of nanostructures since these built-in polarizations are generally quite large, on the order of 1 × 108 to 1 × 1010 V/m. Using these formulations, we come to the prediction that small nanolayered structures are theoretically capable of having larger spontaneous polarizations than their bulk counterparts due to how the dipole moments of the anions and cations within a wurtzite lattice cancel out with one another more in larger structures.
Solvent-Free Mechanochemical Synthesis and Characterization of Nickel Tellurides with Various Stoichiometries: NiTe, NiTe2 and Ni2Te3
Authors: Kristl Matjaž , Gyergyek Sašo , Škapin Srečo D. , Kristl Janja
Published: July 2021

The paper reports the synthesis of nickel tellurides via a mechanochemical method from elemental precursors. NiTe, NiTe2, and Ni2Te3 were prepared by milling in stainless steel vials under nitrogen, using milling times from 1 h to 12 h. The products were characterized by powder X-ray diffraction (pXRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), vibrating sample magnetometer (VSM), UV-VIS spectrometry, and thermal analysis (TGA and DSC). The products were obtained in the form of aggregates, several hundreds of nanometers in size, consisting of smaller nanosized crystallites. The magnetic measurements revealed a ferromagnetic behavior at room temperature. The band gap energies calculated using Tauc plots for NiTe, NiTe2, and Ni2Te3 were 3.59, 3.94, and 3.70 eV, respectively. The mechanochemical process has proved to be a simple and successful method for the preparation of binary nickel tellurides, avoiding the use of solvents, toxic precursors, and energy-consuming reaction conditions.
Liposomes Loaded with Unsaponifiable Matter from Amaranthus hypochondriacus as a Source of Squalene and Carrying Soybean Lunasin Inhibited Melanoma Cells
Authors: Castañeda-Reyes Erick Damian , Gonzalez de Mejia Elvira , Eller Fred Joseph , Berhow Mark A. , Perea-Flores María de Jesús , Dávila-Ortíz Gloria
Published: July 2021

Amaranthus hypochondriacus is a source of molecules with reported health benefits such as antioxidant activity and cancer prevention. The objective of this research was to optimize the conditions for preparing a liposome formulation using amaranth unsaponifiable matter as a source of squalene in order to minimize the particle size and to maximize the encapsulation efficiency of liposomes for carrying and delivering soybean lunasin into melanoma cell lines. Amaranth oil was extracted using supercritical dioxide carbon extraction (55.2 MPa pressure, 80 °C temperature, solvent (CO2)-to-feed (oil) ratio of 20). The extracted oil from amaranth was used to obtain the unsaponifiable enriched content of squalene, which was incorporated into liposomes. A Box–Behnken response surface methodology design was used to optimize the liposome formulation containing the unsaponifiable matter, once liposomes were optimized. Soybean lunasin was loaded into the liposomes and tested on A-375 and B16-F10 melanoma cells. The squalene concentration in the extracted oil was 36.64 ± 0.64 g/ 100 g of oil. The particle size in liposomes was between 115.8 and 163.1 nm; the squalene encapsulation efficiency ranged from 33.14% to 76.08%. The optimized liposome formulation contained 15.27 mg of phospholipids and 1.1 mg of unsaponifiable matter. Cell viability was affected by the liposome formulation with a half-maximum inhibitory concentration (IC50) equivalent to 225 μM in B16-F10 and 215 μM in A-375. The liposomes formulated with lunasin achieved 82.14 ± 3.34% lunasin encapsulation efficiency and improved efficacy by decreasing lunasin IC50 by 31.81% in B16-F10 and by 41.89% in A-375 compared with unencapsulated lunasin.
The Availability and Quality of Food Labelling Components in the Canadian E-Grocery Retail Environment
Authors: Lee Jennifer J. , Ahmed Mavra , Zhang Tianyi , Weippert Madyson V. , Schermel Alyssa , L’Abbé Mary R.
Published: July 2021

Background: Although packaged foods sold in retail stores must follow food labelling regulations, there are no e-grocery food labelling regulations to mandate and standardize the availability and presentation of product information. Therefore, the objective of the study was to evaluate the availability and quality of food labelling components in the Canadian e-grocery retail environment. Methods: A sample of fresh and pre-packaged products was identified on eight leading grocery retail websites in Canada, to assess the availability and quality of food labelling components. Results: Out of 555 product searches, all products were accompanied by product images with front-of-pack images more readily available (96.0%) than back-of-pack (12.4%) and other side panel images (3.1%). The following mandatory nutrition information was available for 61.1% of the products: nutrition facts table (68.8%), ingredient (73.9%), and allergen (53.8%) information. The majority of the nutrition information was available after scrolling down, clicking additionally on the description page, or viewing only as an image. Date markings were not available; packaging material information was available for 2.0% of the products. Conclusions: There was wide variability and inconsistencies in the presentation of food labelling components in the e-grocery retail environment, which can be barriers in enabling Canadians to make informed purchasing decisions.
Experimental and Clinical Evidence of the Effectiveness of Riboflavin on Migraines
Authors: Yamanaka Gaku , Suzuki Shinji , Morishita Natsumi , Takeshita Mika , Kanou Kanako , Takamatsu Tomoko , Morichi Shinichiro , Ishida Yu , Watanabe Yusuke , Go Soken , Oana Shingo , Kawashima Hisashi
Published: July 2021

Riboflavin, a water-soluble member of the B-vitamin family, plays a vital role in producing energy in mitochondria and reducing inflammation and oxidative stress. Migraine pathogenesis includes neuroinflammation, oxidative stress, and mitochondrial dysfunction. Therefore, riboflavin is increasingly being recognized for its preventive effects on migraines. However, there is no concrete evidence supporting its use because the link between riboflavin and migraines and the underlying mechanisms remains obscure. This review explored the current experimental and clinical evidence of conditions involved in migraine pathogenesis and discussed the role of riboflavin in inhibiting these conditions. Experimental research has demonstrated elevated levels of various oxidative stress markers and pro-inflammatory cytokines in migraines, and riboflavin’s role in reducing these marker levels. Furthermore, clinical research in migraineurs showed increased marker levels and observed riboflavin’s effectiveness in reducing migraines. These findings suggest that inflammation and oxidative stress are associated with migraine pathogenesis, and riboflavin may have neuroprotective effects through its clinically useful anti-inflammatory and anti-oxidative stress properties. Riboflavin’s safety and efficacy suggests its usefulness in migraine prophylaxis; however, insufficient evidence necessitates further study.
Brown Seaweed Food Supplementation: Effects on Allergy and Inflammation and Its Consequences
Authors: Olsthoorn Simone E. M. , Wang Xi , Tillema Berend , Vanmierlo Tim , Kraan Stefan , Leenen Pieter J. M. , Mulder Monique T.
Published: July 2021

Multiple health benefits have been ascribed to brown seaweeds that are used traditionally as dietary component mostly in Asia. This systematic review summarizes information on the impact of brown seaweeds or components on inflammation, and inflammation-related pathologies, such as allergies, diabetes mellitus and obesity. We focus on oral supplementation thus intending the use of brown seaweeds as food additives. Despite the great diversity of experimental systems in which distinct species and compounds were tested for their effects on inflammation and immunity, a remarkably homogeneous picture arises. The predominant effects of consumption of brown seaweeds or compounds can be classified into three categories: (1) inhibition of reactive oxygen species, known to be important drivers of inflammation; (2) regulation, i.e., in most cases inhibition of proinflammatory NF-κB signaling; (3) modulation of adaptive immune responses, in particular by interfering with T-helper cell polarization. Over the last decades, several inflammation-related diseases have increased substantially. These include allergies and autoimmune diseases as well as morbidities associated with lifestyle and aging. In this light, further development of brown seaweeds and seaweed compounds as functional foods and nutriceuticals might contribute to combat these challenges.
Slow but Steady—The Responsiveness of Sympathoadrenal System to a Hypoglycemic Challenge in Ketogenic Diet-Fed Rats
Authors: Nedoboy Polina E. , Cohen Myfanwy , Farnham Melissa M.-J.
Published: July 2021

The sympathoadrenal counterregulatory response to hypoglycemia is critical for individuals with type 1 diabetes due to impaired ability to produce glucagon. Ketogenic diets (KD) are an increasingly popular diabetes management tool; however, the effects of KD on the sympathoadrenal response are largely unknown. Here, we determined the effects of KD-induced ketosis on the sympathoadrenal response to a single insulin-induced hypoglycemic challenge. We investigated how a 3 week KD feeding regimen affected the main components of the sympathoadrenal counterregulatory response: adrenal sympathetic nerve activity (ASNA), adrenal gland activity, plasma epinephrine, and brainstem glucose-responsive C1 neuronal activation in anesthetized, nondiabetic male Sprague-Dawley rats. Rats on KD had similar blood glucose (BG) levels and elevated ketone body β-hydroxybutyrate (BHB) levels compared to the control Chow diet group. All KD rats responded to hypoglycemia with a robust increase in ASNA, which was initiated at significantly lower BG levels compared to Chow-fed rats. The delay in hypoglycemia-induced ASNA increase was concurrent with rapid disappearance of BHB from cerebral and peripheral circulation. Adrenal gland activity paralleled epinephrine and ASNA response. Overall, KD-induced ketosis was associated with initiation of the sympathoadrenal response at lower blood glucose levels; however, the magnitude of the response was not diminished.
Association of Patient-Reported Outcomes and Nutrition with Body Composition in Women with Gynecologic Cancer Undergoing Post-Operative Pelvic Radiotherapy: An Observational Study
Authors: Lee Jie , Chen Tze-Chien , Jan Ya-Ting , Li Chi-Jung , Chen Yu-Jen , Wu Meng-Hao
Published: July 2021

Pelvic radiotherapy is associated with gastrointestinal toxicities and deterioration of nutritional status. This study aimed to investigate the association of patient-reported outcomes (PROs) and nutritional status with body composition changes in women who underwent hysterectomy and post-operative radiotherapy for gynecologic cancer. We analyzed data of 210 patients treated with post-operative pelvic radiotherapy for gynecologic cancer between 2013 and 2018. The PRO version of the Common Terminology Criteria for Adverse Events (PRO-CTCAE) was used for gastrointestinal toxicity assessment. The Patient-Generated Subjective Global Assessment (PG-SGA) was used for nutritional assessment. Skeletal muscle index was measured from computed tomography scans at the L3 vertebral level. A reduction in skeletal muscle index ≥ 5% was classified as muscle loss. Odds ratios were calculated through logistic regression models. The PG-SGA score increased from the beginning to the end of radiotherapy (1.4 vs. 3.7, p < 0.001). Patients with PRO-CTCAE scores ≥ 3 had significantly higher PG-SGA scores at the end of radiotherapy than those with PRO-CTCAE scores ≤ 2 (8.1 vs. 2.3, p < 0.001). On multivariable analysis, PRO-CTCAE scores ≥ 3 and PG-SGA scores ≥ 4 at the end of radiotherapy were independently associated with increased risk of muscle loss (odds ratio: 8.81, p < 0.001; odds ratio: 72.96, p < 0.001, respectively). PROs and PG-SGA may be considered as markers of muscle loss after post-operative pelvic radiotherapy for gynecologic cancer.
Diabetes Risk Reduction Diet and Endometrial Cancer Risk
Authors: Esposito Giovanna , Bravi Francesca , Serraino Diego , Parazzini Fabio , Crispo Anna , Augustin Livia S. A. , Negri Eva , La Vecchia Carlo , Turati Federica
Published: July 2021

Diabetes increases endometrial cancer risk. We investigated the role of a diabetes risk reduction diet (DRRD) on the risk of endometrial cancer using data from a multicentric, Italian hospital-based case–control study (1992–2006) enrolling 454 histologically confirmed cases of endometrial cancer and 908 controls matched by age and center. We derived a DRRD score assigning higher scores for higher intakes of cereal fiber, fruit, coffee, polyunsaturated:saturated fatty acid ratio, and nuts and for lower glycemic load and lower intakes of red/processed meat and sugar-sweetened beverages/fruit juices. The odds ratios (OR) of endometrial cancer according to the DRRD score were derived by multiple conditional logistic regression models. The OR for high (DRRD score >24, i.e., third tertile) versus medium–low adherence to the DRRD was 0.73 (95% confidence interval, CI, 0.55–0.97). Similar results were observed after the exclusion of diabetic women (OR 0.75; 95% CI, 0.56–1.00) and allowance for total vegetable consumption (OR 0.80; 95% CI, 0.60–1.07). Inverse associations were observed in most of the analyzed subgroups. The OR for high DRRD combined with high vegetable consumption was 0.45 (95% CI, 0.28–0.73). Our results suggest that diets able to reduce diabetes risk may also reduce endometrial cancer risk. High vegetable consumption combined with high adherence to the DRRD may provide additional benefit in endometrial cancer prevention.
Coinfection of Parvovirus B19 with Influenza A/H1N1 Causes Fulminant Myocarditis and Pneumonia. An Autopsy Case Report
Authors: Callon Domitille , Berri Fatma , Lebreil Anne-Laure , Fornès Paul , Andreoletti Laurent
Published: July 2021

Parvovirus-B19 (PVB19) is a frequent causative agent of myocarditis. For unclear reasons, viral reactivation can cause acute myocarditis, a leading cause of sudden death in the young. Influenza A/H1N1(2009) virus (IAV/H1N1) is known for causing flu/pneumonia, but the heart is rarely involved. Co-infections of cardiotropic viruses are rarely reported and the mechanisms of viral interactions remain unknown. A 5-year old girl had a flu-like syndrome, when she suddenly presented with a respiratory distress and cardiac arrest. At autopsy, the lungs were found haemorrhagic. Lungs’ histology showed severe bronchiolitis, diffuse haemorrhagic necrosis, and mononuclear inflammation. In the heart, a moderate inflammation was found with no necrosis. IAV/H1N1 was detected in nasal and tracheal swabs, lungs, and the heart. The viral load was high in the lungs, but low in the heart. PVB19 was detected in the heart with a high viral load. Viral co-infection increases the risk of severe outcome but the mechanisms of interaction between viruses are poorly understood. In our case, viral loads suggested a reactivated PVB19-induced acute myocarditis during an IAV/H1N1 pneumonia. Viral interactions may involve an IAV/H1N1-induced cytokine storm, with a fulminant fatal outcome. Clinically, our case shows the importance of investigating inflammatory pathways as therapeutic targets.
Alpha Enolase 1 Ubiquitination and Degradation Mediated by Ehrlichia chaffeensis TRP120 Disrupts Glycolytic Flux and Promotes Infection
Authors: Zhu Bing , McBride Jere W.
Published: July 2021

Ehrlichia chaffeensis modulates numerous host cell processes, including gene transcription to promote infection of the mononuclear phagocyte. Modulation of these host cell processes is directed through E. chaffeensis effectors, including TRP120. We previously reported that TRP120 moonlights as a HECT E3 Ub ligase that ubiquitinates host cell transcription and fate regulators (PCGF5 and FBW7) to promote infection. In this study, we identified a novel TRP120 substrate and examined the relationship between TRP120 and α-enolase (ENO1), a metalloenzyme that catalyzes glycolytic pathway substrate dehydration. Immunofluorescence microscopy and coimmunoprecipitation demonstrated interaction between ENO1 and TRP120, and ubiquitination of ENO-1 by TRP120 was detected in vivo and in vitro. Further, ENO-1 degradation was observed during infection and was inhibited by the proteasomal inhibitor bortezomib. A direct role of TRP120 Ub ligase activity in ENO-1 degradation was demonstrated and confirmed by ectopic expression of TRP120 HECT Ub ligase catalytic site mutant. siRNA knockdown of ENO-1 coincided with increased E. chaffeensis infection and ENO-1 knockdown disrupted glycolytic flux by decreasing the levels of pyruvate and lactate that may contribute to changes in host cell metabolism that promote infection. In addition, we elucidated a functional role of TRP120 auto-ubiquitination as an activating event that facilitates the recruitment of the UbcH5 E2 ubiquitin-conjugating enzyme. This investigation further expands the repertoire of TRP120 substrates and extends the potential role of TRP120 Ub ligase in infection to include metabolic reprogramming.
Impact of Selected Small-Molecule Kinase Inhibitors on Lipid Membranes
Authors: Luck Meike , Fischer Markus , Werle Maximilian , Scheidt Holger , Müller Peter
Published: July 2021

Small-molecule protein kinase inhibitors are used for the treatment of various diseases. Although their effect(s) on the respective kinase are generally quite well understood, surprisingly, their interaction with membranes is only barely investigated; even though these drugs necessarily come into contact with the plasma and intracellular membranes. Using biophysical methods such as NMR, ESR, and fluorescence spectroscopy in combination with lipid vesicles, we studied the membrane interaction of the kinase inhibitors sunitinib, erlotinib, idelalisib, and lenvatinib; these drugs are characterized by medium log p values, a parameter reflecting the overall hydrophobicity of the molecules, which is one important parameter to predict the interaction with lipid membranes. While all four molecules tend to embed in a similar region of the lipid membrane, their presence has different impacts on membrane structure and dynamics. Most notably, sunitinib, exhibiting the lowest log p value of the four inhibitors, effectively influences membrane integrity, while the others do not. This shows that the estimation of the effect of drug molecules on lipid membranes can be rather complex. In this context, experimental studies on lipid membranes are necessary to (i) identify drugs that may disturb membranes and (ii) characterize drug–membrane interactions on a molecular level. Such knowledge is important for understanding the efficacy and potential side effects of respective drugs.
Canine Myocytes Represent a Good Model for Human Ventricular Cells Regarding Their Electrophysiological Properties
Authors: Nánási Péter P. , Horváth Balázs , Tar Fábián , Almássy János , Szentandrássy Norbert , Jost Norbert , Baczkó István , Bányász Tamás , Varró András
Published: July 2021

Due to the limited availability of healthy human ventricular tissues, the most suitable animal model has to be applied for electrophysiological and pharmacological studies. This can be best identified by studying the properties of ion currents shaping the action potential in the frequently used laboratory animals, such as dogs, rabbits, guinea pigs, or rats, and comparing them to those of human cardiomyocytes. The authors of this article with the experience of three decades of electrophysiological studies, performed in mammalian and human ventricular tissues and isolated cardiomyocytes, summarize their results obtained regarding the major canine and human cardiac ion currents. Accordingly, L-type Ca2+ current (ICa), late Na+ current (INa-late), rapid and slow components of the delayed rectifier K+ current (IKr and IKs, respectively), inward rectifier K+ current (IK1), transient outward K+ current (Ito1), and Na+/Ca2+ exchange current (INCX) were characterized and compared. Importantly, many of these measurements were performed using the action potential voltage clamp technique allowing for visualization of the actual current profiles flowing during the ventricular action potential. Densities and shapes of these ion currents, as well as the action potential configuration, were similar in human and canine ventricular cells, except for the density of IK1 and the recovery kinetics of Ito. IK1 displayed a largely four-fold larger density in canine than human myocytes, and Ito recovery from inactivation displayed a somewhat different time course in the two species. On the basis of these results, it is concluded that canine ventricular cells represent a reasonably good model for human myocytes for electrophysiological studies, however, it must be borne in mind that due to their stronger IK1, the repolarization reserve is more pronounced in canine cells, and moderate differences in the frequency-dependent repolarization patterns can also be anticipated.
Electrospun Poly(ethylene Terephthalate)/Silk Fibroin Composite for Filtration Application
Authors: Opálková Šišková Alena , Mosnáčková Katarína , Hrůza Jakub , Frajová Jaroslava , Opálek Andrej , Bučková Mária , Kozics Katarína , Peer Petra , Eckstein Andicsová Anita
Published: July 2021

In this study, fibrous membranes from recycled-poly(ethylene terephthalate)/silk fibroin (r-PSF) were prepared by electrospinning for filtration applications. The effect of silk fibroin on morphology, fibers diameters, pores size, wettability, chemical structure, thermo-mechanical properties, filtration efficiency, filtration performance, and comfort properties such as air and water vapor permeability was investigated. The filtration efficiency (FE) and quality factor (Qf), which represents filtration performance, were calculated from penetration through the membranes using aerosol particles ranging from 120 nm to 2.46 μm. The fiber diameter influenced both FE and Qf. However, the basis weight of the membranes has an effect, especially on the FE. The prepared membranes were classified according to EN149, and the most effective was assigned to the class FFP1 and according to EN1822 to the class H13. The impact of silk fibroin on the air permeability was assessed. Furthermore, the antibacterial activity against bacteria S. aureus and E. coli and biocompatibility were evaluated. It is discussed that antibacterial activity depends not only on the type of used materials but also on fibrous membranes’ surface wettability. In vitro biocompatibility of the selected samples was studied, and it was proven to be of the non-cytotoxic effect of the keratinocytes (HaCaT) after 48 h of incubation.
Features of Solution Behavior of Polymer Stars with Arms of Poly-2-alkyl-2-oxazolines Copolymers Grafted to the Upper Rim of Calix[8]arene
Authors: Kirila Tatyana , Amirova Alina , Blokhin Alexey , Tenkovtsev Andrey , Filippov Alexander
Published: July 2021

Star-shaped polymers with arms of block and gradient copolymers of 2-ethyl- and 2-isopropyl-2-oxazolines grafted to the upper rim of calix[8]arene were synthesized by the “grafting from” method. The ratio of 2-ethyl- and 2-isopropyl-2-oxazoline units was 1:1. Molar masses and hydrodynamic characteristics were measured using molecular hydrodynamics and optics methods in 2-nitropropane. The arms of the synthesized stars were short and the star-shaped macromolecules were characterized by compact dimensions and heightened intramolecular density. The influence of the arm structure on the conformation of star molecules was not observed. At low temperatures, the aqueous solutions of the studied stars were not molecular dispersed but individual molecules prevailed. One phase transition was detected for all solutions. The phase separation temperatures decreased with a growth of the content of more hydrophobic 2-isopropyl-2-oxazoline units. It was shown that the way of arms grafting to the calix[8]arene core affects the behavior of aqueous solutions of star-shaped poly-2-alkyl-2-oxazoline copolymers. In the case of upper rim functionalization, the shape of calix[8]arene resembles a plate. Accordingly, the core is less shielded from the solvent and the phase separation temperatures are lower than those for star-shaped poly-2-alkyl-2-oxazolines with lower rim functionalization of the calix[8]arene.
3D-Printed Gelatin Methacrylate Scaffolds with Controlled Architecture and Stiffness Modulate the Fibroblast Phenotype towards Dermal Regeneration
Authors: R. Ibañez Rita I. , do Amaral Ronaldo J. F. C. , Reis Rui L. , Marques Alexandra P. , Murphy Ciara M. , O’Brien Fergal J.
Published: July 2021

Impaired skin wound healing due to severe injury often leads to dysfunctional scar tissue formation as a result of excessive and persistent myofibroblast activation, characterised by the increased expression of α-smooth muscle actin (αSMA) and extracellular matrix (ECM) proteins. Yet, despite extensive research on impaired wound healing and the advancement in tissue-engineered skin substitutes, scar formation remains a significant clinical challenge. This study aimed to first investigate the effect of methacrylate gelatin (GelMA) biomaterial stiffness on human dermal fibroblast behaviour in order to then design a range of 3D-printed GelMA scaffolds with tuneable structural and mechanical properties and understand whether the introduction of pores and porosity would support fibroblast activity, while inhibiting myofibroblast-related gene and protein expression. Results demonstrated that increasing GelMA stiffness promotes myofibroblast activation through increased fibrosis-related gene and protein expression. However, the introduction of a porous architecture by 3D printing facilitated healthy fibroblast activity, while inhibiting myofibroblast activation. A significant reduction was observed in the gene and protein production of αSMA and the expression of ECM-related proteins, including fibronectin I and collagen III, across the range of porous 3D-printed GelMA scaffolds. These results show that the 3D-printed GelMA scaffolds have the potential to improve dermal skin healing, whilst inhibiting fibrosis and scar formation, therefore potentially offering a new treatment for skin repair.
Cellular Automata Modeling of Three-Dimensional Chitosan-Based Aerogels Fiberous Structures with Bezier Curves
Authors: Lebedev Igor , Lovskaya Daria , Mochalova Maria , Mitrofanov Igor , Menshutina Natalia
Published: July 2021

In this work, a cellular automata approach was investigated for modeling three-dimensional fibrous nanoporous aerogel structures. A model for the generation of fibrous structures using the Bezier curves is proposed. Experimental chitosan-based aerogel particles were obtained for which analytical studies of the structural characteristics were carried out. The data obtained were used to generate digital copies of chitosan-based aerogel structures and to assess the accuracy of the developed model. The obtained digital copies of chitosan-based aerogel structures will be used to create digital copies of aerogel structures with embedded active pharmaceutical ingredients (APIs) and further predict the release of APIs from these structures.
Impact of Exposure to Chronic Light–Dark Phase Shifting Circadian Rhythm Disruption on Muscle Proteome in Periparturient Dairy Cows
Authors: McCabe Conor John , Aryal Uma K. , Casey Theresa , Boerman Jacquelyn
Published: July 2021

Muscle tissue serves as a key nutrient reservoir that dairy cows utilize to meet energy and amino acid requirements for fetal growth and milk production. Circadian clocks act as homeostatic regulators so that organisms can anticipate regular environmental changes. The objective of this study was to use liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine how chronic circadian disruption in late gestation affected the muscle tissue proteome. At five weeks before expected calving (BEC), multiparous Holstein cows were assigned to either a control (CON, n = 8) or a 6 h forward phase shift (PS, n = 8) of the light–dark cycle every 3 days. At calving, all animals were exposed to CON light–dark cycles. Muscle biopsies were collected from longissimus dorsi muscles at 21 days BEC and at 21 days postpartum (PP). At p < 0.1, 116 and 121 proteins were differentially abundant between PS and CON at 21 days BEC and 21 days PP, respectively. These proteins regulate beta oxidation and glycolysis. Between pregnancy and lactation, 134 and 145 proteins were differentially abundant in CON and PS cows, respectively (p < 0.1). At both timepoints, PS cows exhibited an oxidative stress signature. Thus, dairy cattle management strategies that minimize circadian disruptions may ensure optimal health and production performance.
Warm Arctic Proglacial Lakes in the ASTER Surface Temperature Product
Authors: Dye Adrian , Bryant Robert , Dodd Emma , Falcini Francesca , Rippin David M.
Published: July 2021

Despite an increase in heatwaves and rising air temperatures in the Arctic, little research has been conducted into the temperatures of proglacial lakes in the region. An assumption persists that they are cold and uniformly feature a temperature of 1 °C. This is important to test, given the rising air temperatures in the region (reported in this study) and potential to increase water temperatures, thus increasing subaqueous melting and the retreat of glacier termini from where they are in contact with lakes. Through analysis of ASTER surface temperature product data, we report warm (>4 °C) proglacial lake surface water temperatures (LSWT) for both ice-contact and non-ice-contact lakes, as well as substantial spatial heterogeneity. We present in situ validation data (from problematic maritime areas) and a workflow that facilitates the extraction of robust LSWT data from the high-resolution (90 m) ASTER surface temperature product (AST08). This enables spatial patterns to be analysed in conjunction with surrounding thermal influences, such as parent glaciers and topographies. This workflow can be utilised for the analysis of the LSWT data of other small lakes and crucially allows high spatial resolution study of how they have responded to changes in climate. Further study of the LSWT is essential in the Arctic given the amplification of climate change across the region.
Temperature Vegetation Dryness Index-Based Soil Moisture Retrieval Algorithm Developed for Geo-KOMPSAT-2A
Authors: Ryu Sumin , Kwon Young-Joo , Kim Goo , Hong Sungwook
Published: July 2021

The Korea Meteorological Administration (KMA) has developed many product algorithms including that for soil moisture (SM) retrieval for the geostationary satellite Geo-Kompsat-2A (GK-2A) launched in December 2018. This was developed through a five-year research project owing to the significance of SM information for hydrological and meteorological applications. However, GK-2A’s visible and infrared sensors lack direct SM sensitivity. Therefore, in this study, we developed an SM algorithm based on the conversion relationships between SM and the temperature vegetation dryness index (TVDI) estimated for various land types in the full disk area using two of GK-2A’s level 2 products, land surface temperature (LST) and normalized difference vegetation index (NDVI), and the Global Land Data Assimilation System (GLDAS) SM data for calibration. Methodologically, various coefficients were obtained between TVDI and SM and used to estimate the GK-2A-based SM. The GK-2A SM algorithm was validated with GLDAS SM data during different periods. Our GK-2A SM product showed seasonal and spatial agreement with GLDAS SM data, indicating a dry-wet pattern variation. Quantitatively, the GK-2A SM showed annual validation results with a correlation coefficient (CC) >0.75, bias <0.1%, and root mean square error (RMSE) <4.2–4.7%. The monthly averaged CC values were higher than 0.7 in East Asia and 0.5 in Australia, whereas RMSE and unbiased RMSE values were <0.5% in East Asia and Australia. Discrepancies between GLDAS and GK-2A TVDI-based SMs often occurred in dry Australian regions during dry seasons due to the high LST sensitivity of GK-2A TVDI. We determined that relationships between TVDI and SM had positive or negative slopes depending on land cover types, which differs from the traditional negative slope observed between TVDI and SM. The KMA is currently operating this GK-2A SM algorithm.
Non-Contact Respiratory Monitoring Using an RGB Camera for Real-World Applications
Authors: Romano Chiara , Schena Emiliano , Silvestri Sergio , Massaroni Carlo
Published: July 2021

Respiratory monitoring is receiving growing interest in different fields of use, ranging from healthcare to occupational settings. Only recently, non-contact measuring systems have been developed to measure the respiratory rate (fR) over time, even in unconstrained environments. Promising methods rely on the analysis of video-frames features recorded from cameras. In this work, a low-cost and unobtrusive measuring system for respiratory pattern monitoring based on the analysis of RGB images recorded from a consumer-grade camera is proposed. The system allows (i) the automatized tracking of the chest movements caused by breathing, (ii) the extraction of the breathing signal from images with methods based on optical flow (FO) and RGB analysis, (iii) the elimination of breathing-unrelated events from the signal, (iv) the identification of possible apneas and, (v) the calculation of fR value every second. Unlike most of the work in the literature, the performances of the system have been tested in an unstructured environment considering user-camera distance and user posture as influencing factors. A total of 24 healthy volunteers were enrolled for the validation tests. Better performances were obtained when the users were in sitting position. FO method outperforms in all conditions. In the fR range 6 to 60 breaths/min (bpm), the FO allows measuring fR values with bias of −0.03 ± 1.38 bpm and −0.02 ± 1.92 bpm when compared to a reference wearable system with the user at 2 and 0.5 m from the camera, respectively.
Estimation of Stroke Volume Variance from Arterial Blood Pressure: Using a 1-D Convolutional Neural Network
Authors: Kwon Hye-Mee , Seo Woo-Young , Kim Jae-Man , Shim Woo-Hyun , Kim Sung-Hoon , Hwang Gyu-Sam
Published: July 2021

Background: We aimed to create a novel model using a deep learning method to estimate stroke volume variation (SVV), a widely used predictor of fluid responsiveness, from arterial blood pressure waveform (ABPW). Methods: In total, 557 patients and 8,512,564 SVV datasets were collected and were divided into three groups: training, validation, and test. Data was composed of 10 s of ABPW and corresponding SVV data recorded every 2 s. We built a convolutional neural network (CNN) model to estimate SVV from the ABPW with pre-existing commercialized model (EV1000) as a reference. We applied pre-processing, multichannel, and dimension reduction to improve the CNN model with diversified inputs. Results: Our CNN model showed an acceptable performance with sample data (r = 0.91, MSE = 6.92). Diversification of inputs, such as normalization, frequency, and slope of ABPW significantly improved the model correlation (r = 0.95), lowered mean squared error (MSE = 2.13), and resulted in a high concordance rate (96.26%) with the SVV from the commercialized model. Conclusions: We developed a new CNN deep-learning model to estimate SVV. Our CNN model seems to be a viable alternative when the necessary medical device is not available, thereby allowing a wider range of application and resulting in optimal patient management.
Development of Hybrid Piezoelectric-Fibre Optic Composite Patch Repair Solutions
Authors: Lambinet Florian , Sharif Khodaei Zahra
Published: July 2021

This paper proposes a hybrid structural health monitoring (SHM) solution for a smart composite patch repair for aircraft structures based on piezoelectric (PZT) and fibre optic (FO) sensors to monitor the integrity of a the bondline and detect any degradation. FO sensors are used to acquire guided waves excited by PZT transducers to allow the advantages of both sensor technologies to be utilised. One of the main challenges of guided wave based detection methodologies is to distinguish the effect of temperature on the propagating waves, from that of an existing damage. In this research, the application of the hybrid SHM system is tested on a composite step sanded repair coupon under operational condition (temperature variation) representative of an aircraft for the first time. The sensitivity of the embedded FO sensor in recording the strain waves is compared to the signals acquired by PZT sensors under varying temperature. A novel compensation algorithm is proposed to correct for the effect of the temperature on the embedded FO sensor spectrum in the hybrid set-up. The repaired specimen is then impacted with a drop mass to cause barely visible impact damage (BVID). The hybrid SHM system is then used to detect the damage, and its diagnosis results are compared to a PZT only based smart repair solution. The results show promising application of the hybrid solution for monitoring bondline integrity as well as highlighting challenges of the embedding of FO sensors for a reliable and repeatable diagnosis.