bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats
Authors: Rubio Luis A. , Chiesa Giulia
Published: February 2025

Background: Previous work has shown that the mostly beneficial modulation of intestinal microbiota generally found with legume-based diets is likely to be due, at least in part, to their constituent protein components. Objectives: The faecal microbiota composition was studied in rats fed diets differing only in their constituent proteins. Methods: Rats (n = 10/group) were fed for 28 days diets based in milk [(lactalbumin (LA), casein (CAS)], or white lupin (Lupinus albus) protein isolate (LPI). Results: Significant differences among the three groups in bacteria composition and functionality were found by both qPCR and Illumina sequencing analysis. Significant (p < 0.01) differences were found by ANOSIM and Discriminant Analysis among groups at the family, genus and species levels in both microbiota composition and functionality. A number of groups able to explain the differences between animal (casein, lactalbumin) and lupin proteins were revealed by LEfSe and PCA analysis. Specifically, feeding the CAS diet resulted in lower Bifidobacteria and Lactobacilli compared to the other diets, and the LPI diet gave place to lower Enterobacteria than CAS, and lower Escherichia/Shigella than LA and CAS. Differences in the LA group were attributable to Bifidobacterium spp., Collinsella spp. (in particular C. stercoris), Bacteroides spp., Eubacterium spp. (in particular E. dolichum), Roseburia spp. (in particular R. faecis), and Oscillospira spp. In the case of the CAS group, the organisms were Parabacteroides spp., Blautia spp., Enterobacteriaceae spp., Turicibacter spp., species from Christenellaceae, species from Alphaproteobacteria and Mogibacteriaceae, Coprobacillus spp. and Dorea spp. In the case of the LPI group, the organisms were Lactobacillus spp. (Lactobacillus spp. and L. reuteri), species from Clostridiaceae, species from Peptostreptococcaceae, species from Erysipelotrichaceae, and Adlercreutzia spp. Conclusions: Based on the results obtained, LPI is likely to beneficially modulate the intestinal microbiota composition in rats. Additionally, LA-based diet was associated to a healthier microbiota composition than CAS, although the CAS diet also modulated the intestinal microbiota to a composition compatible with improved bowel movement frequency and lipid metabolism.
An Online Nutrition Education Program Targeting Intentions and Related Determinants Towards Dietary Supplement Use: An Application of the Theory of Planned Behavior
Authors: Daher Jana , Mountjoy Margo , El Khoury Dalia
Published: February 2025

Background/Objectives: Dietary supplement use among varsity athletes is influenced by various psychological and social factors, yet there is limited evidence on the effectiveness of educational interventions in influencing these determinants. The aim of this study was to determine the effects of an online nutrition education program on improvements in intentions and related determinants towards the use of dietary supplements in varsity athletes at the University of Guelph in Canada. The theory of planned behavior served as the theoretical framework for examining these determinants as predictors of behavior change. Methods: A randomized wait-list controlled trial was conducted on a total of 30 varsity athletes, randomized into experimental (n = 18) and control (n = 12) groups. The experimental group received access to an online nutrition education program focused on sports nutrition and dietary supplements, while the control group did not have access during the study. Results: There was a significant (p < 0.05) intervention effect on varsity athletes’ attitudes (Mean1(control) = 13.17; Mean1(experimental) = 13.56; Mean2(control) = 13.92; Mean2(experimental) = 12.11), perceived behavioral control (Mean1(control) = 15.92; Mean1(experimental) = 16.11; Mean2(control) = 16.33; Mean2(experimental) = 18.39), and intentions (Mean1(control) = 12.5; Mean1(experimental) = 12.89; Mean2(control) = 11.58; Mean2(experimental) = 9.44) towards dietary supplement use. No significant changes were made to descriptive and injunctive norms. Conclusions: These findings suggest that this nutrition education program significantly improved intentions and related determinants towards dietary supplement use in varsity athletes. The absence of a successful change in subjective norm should be a focus for similar future interventions.
Dynamic Nutrition Strategies for Anorexia Nervosa: Marker-Based Integration of Calories and Proteins
Authors: Dozio Eugenia , Alonge Martina , Tori Gianluca , Caumo Andrea , Russo Rina Giuseppa , Scuttari Edoardo , Fringuelli Leonardo , Terruzzi Ileana
Published: February 2025

Background/Objectives: Anorexia nervosa (AN) is a severe psychiatric disorder characterized by profound nutritional deficits and significant alterations in body composition, cellular integrity, and hydration. Nutritional rehabilitation is critical not only for weight restoration but also for improving body composition and metabolic functions. However, optimal strategies for integrating caloric and protein intake to achieve balanced recovery remain underexplored. This study aims to evaluate the interactions between caloric/protein intake and time on quantitative (weight and BMI) and qualitative (body composition and cellular health) outcomes, and to identify markers that predict recovery trajectories and guide personalized nutritional interventions. Methods: This retrospective observational study analyzed 79 patients with AN admitted to Villa Miralago for six months of nutritional rehabilitation. Anthropometric and body composition parameters—including body weight (BW), body mass index (BMI), fat mass (FM), fat-free mass (FFM), body cell mass (BCM), phase angle (PA), and hydration markers (TBW and ECW)—were assessed at baseline (T0), 3 months (T1), and 6 months (T2). Generalized Estimating Equations (GEEs) were used to evaluate the effects of caloric and protein intake over time. Results: Significant increases in BW (+6.54 kg, p < 0.0001) and BMI (+2.47 kg/m2, p < 0.0001) were observed, alongside improvements in FM, FFM, and BCM. PA increased significantly (+0.47°, p < 0.0001), indicating enhanced cellular health. TBW increased (+1.58 L, p < 0.0001), while ECW% decreased, reflecting improved fluid distribution. Caloric intake predominantly influenced early fat mass recovery, while protein intake was crucial for preserving lean tissues and promoting cellular regeneration. Interaction effects between caloric/protein intake and time revealed dynamic changes in body composition, underscoring the need for adaptive strategies. Conclusions: This study highlights the importance of a dynamic, marker-based approach to nutritional rehabilitation in AN. Integrating caloric and protein intake with advanced body composition and hydration markers enables personalized interventions and balanced recovery, shifting AN treatment toward a focus on qualitative improvements overweight restoration alone.
Impact of Different Low-Volume Concurrent Training Regimens on Cardiometabolic Health, Inflammation, and Fitness in Obese Metabolic Syndrome Patients
Authors: Reljic Dejan , Herrmann Hans Joachim , Neurath Markus Friedrich , Zopf Yurdagül
Published: February 2025

Background/Objectives: Evidence supports the benefits of concurrent training (CT), which combines endurance and resistance exercises, for enhancing health and physical fitness. Recently, low-volume, time-efficient exercise approaches such as low-volume high-intensity interval training (LOW-HIIT), whole-body electromyostimulation (WB-EMS), and single-set resistance training (1-RT) have gained popularity for their feasibility and efficacy in improving various health outcomes. This study investigated the effects of low-volume CT, focusing on (1) whether exercise order affects cardiometabolic health, inflammation, and fitness adaptations and (2) which combination, LOW-HIIT plus WB-EMS or LOW-HIIT plus 1-RT, yields better results. Methods: Ninety-three obese metabolic syndrome (MetS) patients undergoing caloric restriction were randomly assigned to four groups performing the different low-volume CT protocols over 12 weeks. Outcomes included cardiometabolic, inflammatory, and fitness parameters. Results: In both combinations, no significant differences were found regarding exercise order. However, the pooled LOW-HIIT and 1-RT group achieved superior improvements in blood pressure, blood lipids, inflammation markers (CRP, hsCRP), the MetS severity score, and overall fitness compared to the LOW-HIIT and WB-EMS combination. Compared to previous studies using these modalities individually, LOW-HIIT plus 1-RT appeared to further reduce inflammation, whereas LOW-HIIT combined with WB-EMS was less effective for cardiometabolic health, potentially due to interference effects between modalities. Conclusions: While LOW-HIIT plus WB-EMS appears to be a viable option for individuals unable to perform traditional resistance training, the findings suggest prioritizing LOW-HIIT plus 1-RT to maximize health outcomes. These findings highlight the importance of tailored exercise prescriptions and the need for further research into optimizing CT protocols for diverse populations.
The Effect of Flavonoids and Topiramate on Glucose Carbon Metabolism in a HepG2 Steatosis Cell Culture Model: A Stable Isotope Study
Authors: Ma Li , Lu Qing-Yi , Lim Shu , Han Guang , Boros Laszlo G. , Desai Mina , Yee Jennifer K.
Published: February 2025

Background: Insufficient treatment options are available for metabolic dysfunction-associated steatotic liver disease (MASLD). Flavonoids and topiramate have been studied for weight loss but need investigation into their effects on liver metabolism. This study’s aim was to examine the effects of flavonoids or topiramate on glucose metabolic carbon flux in a cell culture model of steatosis. Methods: Steatosis was induced in HepG2 cells through exposure to oleic acid (OA, 0.5 mml/L) conjugated to bovine serum albumin (2:1). Additionally, 50% U13C-glucose was supplied in the medium as a stable isotope tracer. Cells were treated with DMSO, 10 μM of naringenin, morin, silibinin, or topiramate (44 μM) for 72 h. A non-steatotic, untreated HepG2 cell control was included. Cell extracts were analyzed by gas chromatography/mass spectrometry and mass isotopomer distribution analysis for glycogen synthesis, de novo fatty acid synthesis, tricarboxylic acid (TCA) cycle activity, and ribose synthesis. Groups were compared by ANOVA with Tukey’s pair-wise testing. Results: Compared to untreated HepG2 controls, OA-exposed steatotic cells exhibited increased lipid accumulation by ORO staining (1.6-fold), enhanced palmitate de novo synthesis, reduced pyruvate carboxylase/pyruvate dehydrogenase (PC/PDH) ratio, and reduced ribose synthesis. Treatment with topiramate or silibinin ameliorated the lipid accumulation (1.3-fold) and mitigated enhancement of de novo synthesis. Morin-treated cells exhibited enhanced de novo synthesis but suppressed ribose synthesis. Conclusions: Potential mechanisms of reduced lipid accumulation by topiramate and silibinin may include suppression of palmitate de novo synthesis and a relative decrease in carbon flux through the PDH pathway. Further studies are needed on potential utility in MASLD based on their specific metabolic effects.
Role of Ginseng and L-Carnitine in Modulating Exercise Endurance and Oxidative Stress in Rats
Authors: Posridee Kakanang , Kupittayanant Sajeera , Rachnavy Pornthep , Oonsivilai Anant , Oonsivilai Ratchadaporn
Published: February 2025

Ginseng and L-carnitine are natural compounds often used as dietary supplements to enhance athletic performance. However, their combined effects on exercise endurance remain unclear. Objectives: This study aimed to investigate the effects of ginseng extract and L-carnitine supplementation on exercise endurance in a rat model. Methods: Male Wistar rats were divided into 10 groups (n = 5 per group): control, ginseng extract (250 and 500 mg/kg/day), L-carnitine (250 and 500 mg/kg/day), and combined treatment. Half of the groups underwent a 16-day exercise training program of swimming without loading. Exercise endurance was assessed using a tail-suspended forced swimming test. Relative organ weight, glycogen content, blood biochemistry, and gene expression were analyzed. Results: Both ginseng extract and L-carnitine supplementation significantly increased exercise endurance, particularly in the exercise group. Ginseng extract and L-carnitine also increased liver glycogen content and upregulated the expression of AMPKα1 and PGC-1α genes in the liver and muscle. In addition, both supplements reduced oxidative stress by decreasing MDA levels and increasing SOD activity. Conclusions: Ginseng extract and L-carnitine supplementation may enhance exercise endurance by improving energy metabolism, reducing oxidative stress, and upregulating key genes involved in mitochondrial biogenesis.
Patient and Family Involvement in Nursing Bedside Handover: A Qualitative Descriptive Study of Consumer Perceptions of Nursing Care
Authors: Ghosh Manonita , O’Connell Beverly , Nguyen Hien Thi , Coventry Linda , Towell-Barnard Amanda , Gallagher Olivia , Gullick Karen , Gent Lucy , Saunders Rosemary
Published: February 2025

Background/Objectives: Patient and family involvement in bedside handover is a requirement of the national standards on patient safety and quality in Australia. To ensure patient-and-family-centred care, it is essential to understand how patients and families perceive their involvement in nursing bedside handover and what difficulties they face when participating. This study aimed to explore patient and family perceptions of their involvement in nursing bedside handover. Methods: We employed a qualitative descriptive study design with in-depth and semi-structured interviews. Using purposive and convenience sampling, 24 patients and family members were recruited from two adult hospitals in Western Australia between November 2021 and February 2022. The data were thematically analysed. Results: Participants had mixed experiences that overlapped with their individual perceptions, needs, and experiences. Their responses were grouped into three major themes with sub-themes: (1) discovering new nursing care approaches; (2) seeing the value of involvement in bedside handover; and (3) barriers hindering patient and family involvement in bedside handover. The findings revealed that patients and families valued their involvement in nursing bedside handover. However, several factors challenged their participation, including a lack of awareness about their right to participate, the timing of handovers, the nurse’s approach, and fear of asking questions. Conclusions: The findings serve as a guide for evidence-based practice and may significantly influence policy and practice in nursing bedside handover, potentially enhancing patient-and-family-centred care. While considered best practice, the consistent involvement of patients and their families in nursing bedside handover is not routinely achieved and is implemented to varying extents.
Dietary Intake and Body Composition of Fixed-Shift Workers During the Climacteric: An Intervention Study with Exogenous Melatonin
Authors: Luz Cristina S. S. , Nehme Patricia X. S. A. , Saraiva Susy , D’Aurea Carolina V. R. , Amaral Fernanda G. , Cipolla-Neto Jose , Marqueze Elaine C. , Moreno Claudia R. C.
Published: February 2025

Poor sleep quality is associated with unhealthy dietary choices and worse body composition among night-shift workers and women during the climacteric period. This study aimed to evaluate the effect of exogenous melatonin administration on the dietary intake and body composition in healthcare workers exposed to fixed-shift work during the climacteric period. A phase II randomized, double-blind, placebo-controlled clinical trial of climacteric women working fixed morning, afternoon, or night shifts was conducted. Baseline data collection included sociodemographic information, sleep parameters, dietary intake, and body composition using bioelectrical impedance. Participants received either 0.3 mg of melatonin or a placebo for three months. Baseline total caloric and carbohydrate intake was higher among night-shift workers than afternoon-shift workers, though afternoon-shift workers a had higher body weight and fat percentage. Post-intervention, caloric intake differences dissipated due to reduced intake among night-shift workers and increased intake among afternoon-shift workers, independent of melatonin or placebo administration. However, differences in body composition persisted. Postmenopausal participants showed reduced fat mass, while premenopausal participants experienced an increase, regardless of intervention. The results suggest that physiological nighttime melatonin doses, administered on non-consecutive nights for three months, were ineffective for changing dietary intake or body composition.
Disparities in Immunotherapy Use Prior to First-Line Approval for Metastatic Hepatocellular Carcinoma in the United States
Authors: Shah Manav , Khalid Abdullah , Standring Oliver , Amini Neda , Demyan Lyudmyla , Koti Shruti , Gazzara Emma , Wu Grace , Vithlani Nandan , DePeralta Danielle , Gholami Sepideh , Weiss Matthew
Published: February 2025

Background/Objectives: Treatment options for advanced HCC are limited. Immunotherapy, a promising new therapeutic, has recently been incorporated as a first-line systemic treatment. This study evaluates immunotherapy uptake and disparities in its adoption prior to first-line approval, which are currently unknown. Methods: Patients diagnosed with stage IV HCC between 2014 and 2019 were identified in the National Cancer Database (NCDB). Multivariable logistic regression analysis was performed for demographic and clinical variables to determine their association with the receipt of immunotherapy. Results: A total of 18,248 patients were diagnosed with stage IV HCC, of which 977 (5.35%) received immunotherapy. From 2014 to 2019, the rate of immunotherapy uptake increased each year, with an approximate 25-fold increase overall. On multivariable analysis, patients with a more recent period of diagnosis (OR: 30.19, 95% CI: 16.47–55.33), with private insurance (OR: 2.56, 95% CI: 1.62–4.04), with treatment at an academic or research center (OR: 1.97, 95% CI: 1.37–2.82), or with an income ≥ USD 63,333 (OR: 1.27, 95% CI: 1.01–1.59) were more likely to receive immunotherapy. Conclusions: Various demographic factors impact the receipt of immunotherapy in the pre-approval setting, such as income, insurance status, and treatment center. As immunotherapy use will likely further expand and these disparities may potentially persist following first-line approval, strategies to mitigate them are especially important.
Harnessing Epigenetics: Innovative Approaches in Diagnosing and Combating Viral Acute Respiratory Infections
Authors: Saha Ankita , Ganguly Anirban , Kumar Anoop , Srivastava Nityanand , Pathak Rajiv
Published: February 2025

Acute respiratory infections (ARIs) caused by viruses such as SARS-CoV-2, influenza viruses, and respiratory syncytial virus (RSV), pose significant global health challenges, particularly for the elderly and immunocompromised individuals. Substantial evidence indicates that acute viral infections can manipulate the host’s epigenome through mechanisms like DNA methylation and histone modifications as part of the immune response. These epigenetic alterations can persist beyond the acute phase, influencing long-term immunity and susceptibility to subsequent infections. Post-infection modulation of the host epigenome may help distinguish infected from uninfected individuals and predict disease severity. Understanding these interactions is crucial for developing effective treatments and preventive strategies for viral ARIs. This review highlights the critical role of epigenetic modifications following viral ARIs in regulating the host’s innate immune defense mechanisms. We discuss the implications of these modifications for diagnosing, preventing, and treating viral infections, contributing to the advancement of precision medicine. Recent studies have identified specific epigenetic changes, such as hypermethylation of interferon-stimulated genes in severe COVID-19 cases, which could serve as biomarkers for early detection and disease progression. Additionally, epigenetic therapies, including inhibitors of DNA methyltransferases and histone deacetylases, show promise in modulating the immune response and improving patient outcomes. Overall, this review provides valuable insights into the epigenetic landscape of viral ARIs, extending beyond traditional genetic perspectives. These insights are essential for advancing diagnostic techniques and developing innovative treatments to address the growing threat of emerging viruses causing ARIs globally.
Dynamic Modulation of IRE1α-XBP1 Signaling by Adenovirus
Authors: Jang Yumi , Bunz Fred
Published: February 2025

The abundant production of foreign proteins and nucleic acids during viral infection elicits a variety of stress responses in host cells. Viral proteins that accumulate in the endoplasmic reticulum (ER) can trigger the unfolded protein response (UPR), a coordinated signaling program that culminates in the expression of downstream genes that collectively restore protein homeostasis. The model pathogen adenovirus serotype 5 (HAdV5) activates the UPR via the signaling axis formed by inositol-requiring enzyme type 1 (IRE1α) and the X-box binding protein 1 (XBP1), a transcription factor required for immune function. Recent studies have suggested that IRE1α-XBP1 activity supports adenovirus replication. Here, we show that HAdV5 exerted opposing effects on IRE1α and XBP1. IRE1α was activated in response to HAdV5, but the production of the XBP1 isoform, XBP1s, was post-transcriptionally blocked. The tumor suppressor p53, which is eliminated by HAdV5 after infection, inhibited IRE1α activation. The de-repression of IRE1α following the degradation of p53 conceivably reflects a novel antiviral mechanism, which HAdV5 ultimately evades by co-opting IRE1α and suppressing XBP1s. Our findings illustrate the opposing mechanisms used by adenoviruses and their host cells to exert control over the UPR, a critical determinant of cell fate.
Oncolytic Viruses in Ovarian Cancer: Where Do We Stand? A Narrative Review
Authors: Borella Fulvio , Carosso Marco , Chiparo Maria Pia , Ferraioli Domenico , Bertero Luca , Gallio Niccolò , Preti Mario , Cusato Jessica , Valabrega Giorgio , Revelli Alberto , Marozio Luca , Cosma Stefano
Published: February 2025

Ovarian cancer (OC) remains the most lethal gynecologic malignancy with limited effective treatment options. Oncolytic viruses (OVs) have emerged as a promising therapeutic approach for cancer treatment, capable of selectively infecting and lysing cancer cells while stimulating anti-tumor immune responses. Preclinical studies have demonstrated significant tumor regression and prolonged survival in OC models using various OVs, such as herpes simplex. Early-phase clinical trials have shown a favorable safety profile, though the impact on patient survival has been modest. Current research focuses on combining OVs with other treatments like immune checkpoint inhibitors to enhance their efficacy. We provide a comprehensive overview of the current understanding and future directions for utilizing OVs in the management of OC.
A New Trick of Old Dogs: Can Kappa Opioid Receptor Antagonist Properties of Antidepressants Assist in Treating Treatment-Resistant Depression (TRD)?
Authors: Schreiber Shaul , Keidan Lee , Pick Chaim G.
Published: February 2025

Background/Objectives: Approximately one in five individuals will experience major depressive disorder (MDD), and 30% exhibit resistance to standard antidepressant treatments, resulting in a diagnosis of treatment-resistant depression (TRD). Historically, opium was used effectively to treat depression; however, when other medications were introduced, its use was discontinued due to addiction and other hazards. Recently, kappa opioid receptor (KOR) antagonism has been proposed as a potential mechanism for treating TRD. The main research question is whether commonly used psychotropic medications possess KOR antagonist properties and whether this characteristic could contribute to their efficacy in TRD. Methods: We investigated the antinociceptive effects of many psychotropic medications and their interactions with the opioid system. Mice were tested with a hotplate or tail-flick after being injected with different doses of these agents. Results: The antidepressants mianserin and mirtazapine (separately) induced dose-dependent antinociception, each yielding a biphasic dose–response curve. Similarly, the antidepressant venlafaxine produced a potent effect and reboxetine produced a weak effect. The antipsychotics risperidone and amisulpride exhibited a dose-dependent antinociceptive effect. The sedative–hypnotic zolpidem induced a weak bi-phasic dose-dependent antinociceptive effect. All seven psychotropic medications elicited antinociception, which was reversed by the non-selective opiate antagonist naloxone and, separately, by the kappa-selective antagonist Nor-BNI. Conclusions: Clinical studies are mandatory to establish the potential efficacy of augmentation of the treatment with antidepressants with these drugs in persons with treatment-resistant depression and the optimal dosage of medications prescribed. We suggest a possible beneficial effect of antidepressants with kappa antagonistic properties.
Antimicrobial and Antibiofilm Activity of Chitosan Nanoparticles Against Staphylococcus aureus Strains Isolated from Bovine Mastitis Milk
Authors: Godoy Carlos Alarcón , Balic Iván , Moreno Adrián A. , Diaz Oscar , Arenas Colarte Carla , Bruna Larenas Tamara , Gamboa Alexander , Caro Fuentes Nelson
Published: February 2025

Background/Objectives: Bovine mastitis (BM), a prevalent and economically burdensome bacterial infection affecting dairy cattle, poses a significant challenge to the dairy industry. The traditional approach to combating BM, relying heavily on antibiotics, faces growing concerns due to the increasing antibiotic resistance exhibited by pathogens. The objective of this study was to evaluate and determine the antimicrobial and anti-biofilm potential of chitosan nanoparticles (NQo) on S. aureus strains isolated from milk samples obtained from dairy areas in southern Chile from cows diagnosed with BM. Methods: NQo were synthesized using the ionotropic gelation method and thoroughly characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Results: The NQo exhibit a robust positive charge (Z-potential of +55.4 ± 2.5 mV) and an exceptionally small size (20.3 ± 3.2 nm). This unique combination of properties makes NQo particularly well-suited for targeting and interacting with bacterial pathogens. To assess the effectiveness of NQo against BM, we conducted a series of experiments using a Staphylococcus aureus strain isolated from milk samples of cows diagnosed with BM in southern Chile. NQo demonstrated a remarkable ability to inhibit bacterial proliferation and effectively modulate biofilm formation in the S. aureus strains. Furthermore, the performance of NQo in comparison to established antibiotics like ampicillin and gentamicin strongly suggests that these nanoparticles hold immense potential as an attractive alternative for the control, prevention, and/or treatment of BM. Conclusions: NQo exhibit both antimicrobial and antibiofilm activity against a clinically relevant BM pathogen. Further investigations are necessary to develop a hydrogel formulation optimized for effective delivery to the target diseased tissue.
Time, Risk and Control in Musical Performance Practices
Authors: Garda Michela
Published: February 2025

Time, control and risk are interrelated concepts that provide a valuable framework for exploring the connections among various performative practices and their cultural functions. By referencing sports, circus arts, and musical performance, this article examines the similarities and differences between musical reproductive performance and improvisation. It focuses on the concept of transformational processes through analogies.
SVM-Based Optical Detection of Retinal Ganglion Cell Apoptosis
Authors: Kulmaganbetov Mukhit , Bevan Ryan , Want Andrew , Anantrasirichai Nantheera , Achim Alin , Albon Julie , Morgan James
Published: February 2025

Background: Retinal ganglion cell (RGC) loss is crucial in eye diseases like glaucoma. Axon damage and dendritic degeneration precede cell death, detectable within optical coherence tomography (OCT) resolution, indicating their correlation with neuronal degeneration. The purpose of this study is to evaluate the optical changes of early retinal degeneration. Methods: The detection of optical changes in the axotomised retinal explants was completed in six C57BL/6J mice. OCT images were acquired up to 120 min from enucleation. A grey-level co-occurrence-based texture analysis was performed on the inner plexiform layer (IPL) to monitor changes in the optical speckles using a principal component analysis (PCA) and a support vector machine (SVM). In parallel tests, retinal transparency was confirmed by a comparison of the modulation transfer functions (MTFs) at 0 and 120 min. Results: Quantitative confirmation by analysis of the MTFs confirmed the non-degradation of optical transparency during the imaging period: MTF (fx) = 0.267 ± 0.02. Textural features in the IPL could discriminate between the optical signals of RGC degeneration. The mean accuracy of the SVM classification was 86.3%; discrimination was not enhanced by the combination of the SVM and PCA (81.9%). Conclusions: Optical changes in the IPL can be detected using OCT following RGC axotomy. High-resolution OCT can provide an index of retinal neuronal integrity and its degeneration.
Puccinia triticina and Salicylic Acid Stimulate Resistance Responses in Triticum aestivum Against Diuraphis noxia Infestation
Authors: Bilal Huzaifa , Boshoff Willem Hendrik Petrus , Mohase Lintle
Published: February 2025

Wheat plants encounter both biotic and abiotic pressure in their surroundings. Among the biotic stress factors, the Russian wheat aphid (RWA: Diuraphis noxia Kurdjumov) decreases grain yield and quality. The current RWA control strategies, including resistance breeding and the application of aphicides, are outpaced and potentially environmentally harmful. Alternatively, priming can stimulate defence responses to RWA infestation. This study investigated the priming potential of two priming agents, avirulent Puccinia triticina (Pt) isolates and salicylic acid (SA), against RWA infestation. The priming effect of Pt isolates and SA in reducing RWA-induced leaf damage and increased antioxidant activities is an indication of defence responses. Selected South African wheat cultivars and Lesotho landraces, grown under greenhouse conditions, were inoculated with Pt isolates (UVPt13: avirulent, UVPt26: virulent) and treated with SA at the seedling or booting stages. The leaf damage rating score was used for phenotyping. The antioxidant-mediated defence responses were evaluated in three selected cultivars for further priming investigation. Our results revealed that the priming agents significantly reduced the leaf damage in most cultivars at both growth stages, and UVPt13 and SA priming significantly (p ≤ 0.05) increased superoxide dismutase, peroxidase, and ascorbate peroxidase activities. However, catalase activity exhibited a more pronounced decline in plants treated with the UVPt13 isolate. The Pt isolate priming was more efficient than the SA application. However, it is crucial to investigate the potential of effectors from the avirulent Pt isolate to prime wheat plants for resistance against RWA infestation. This could contribute to developing strategies to enhance crop protection and relieve pest pressure in wheat production.
Establishment of a Highly Efficient In Vitro Regeneration System for Nandina domestica ‘Firepower’
Authors: Zhao Xin , Dong Hao , Li Yanhua , Zhang Xinxin , Ning Yajing , Cui Chengpeng , Li Shujuan
Published: February 2025

Nandina domestica ‘Firepower’ is one of the most popular colorful foliage species in landscaping. However, it is currently propagated mainly by seeding and cuttings, with a low reproduction coefficient, hindering the cultivation of this species. Therefore, establishing an in vitro regeneration system would be beneficial for the industrialized production of Nandina domestica ‘Firepower’. In this study, an ex vivo regeneration system was established using the direct organogenesis pathway. In early April, the new shoots of Nandina domestica ‘Firepower’ were selected, and the stem segments of 1~2 cm were cut as the disinfection materials for the explants. The optimal formulation for inducing axillary shoots was 1/2 MS + 1.5 mg L−1 6-benzylaminopurine (BA) + 0.3 mg L−1 indole-3-butric acid (IBA). The optimal formulation for the differentiation and proliferation of axillary shoots was 1/2 MS + 1.5 mg L−1 BA + 0.01 mg L−1 IBA with a multiplicity of proliferation of 9.22. We determined that the rooting of axillary shoots required a combination of IBA, naphthalene acetic acid (NAA), and activated carbon (AC). The optimal formulation for rooting was 1/2 MS + 0.2 mg L−1 NAA + 0.3 mg L−1 IBA + 0.2 mg L−1 AC. After a two-day hardening period for tissue-cultured plantlets, a substrate consisting of peat soil, vermiculite, and perlite at a ratio of 2:2:1 was determined to be the optimal cultivation formulation. This system provides a framework for the industrialized production of Nandina domestica ‘Firepower’.
Comparative Proteomic Analysis of Popcorn Genotypes Identifies Differentially Accumulated Proteins Associated with Resistance Pathways to Southern Leaf Blight Disease
Authors: Corrêa Caio Cézar Guedes , Barroso Tatiana Santos , Xavier Lucas Rodrigues , Pinto Vitor Batista , Reis Ricardo Souza , Pena Guilherme Ferreira , Santa-Catarina Claudete , Vivas Marcelo , do Amaral Júnior Antonio Teixeira , Silveira Vanildo
Published: February 2025

Southern leaf blight (SLB), caused by Bipolaris maydis, poses a significant threat to maize and popcorn production. To understand the molecular mechanisms underlying SLB resistance, we conducted a high-throughput proteomic analysis comparing SLB-resistant (L66) and SLB-susceptible (L51) popcorn genotypes at four and ten days after inoculation (DAI). A total of 717 proteins were identified, with 151 differentially accumulated proteins (DAPs) between the genotypes. Eighteen DAPs exhibited the same regulatory pattern in both the SLB-resistant and SLB-susceptible genotypes at four (R4/S4) and ten (R10/S10) DAI. The protein-protein interaction (PPI) network of differentially accumulated proteins (DAPs) linked to SLB resistance and susceptibility enriched specific metabolic pathways in the SLB response, including photosynthesis, ribosome, ascorbate and aldarate metabolism, glutathione metabolism, and carbon metabolism. Proteins such as photosystem II 11 kD protein (B4FRJ4, PSB27-1), which was up-regulated at both time points (R4/S4 and R10/S10), and 60S acidic ribosomal protein P0 (A0A1D6LEZ7, RPP0B), which was unique to the resistant genotype at both time points (R4 and R10), highlighted the importance of maintaining photosynthetic efficiency and protein synthesis during pathogen attack. Additionally, dehydroascorbate reductase like-3 (B4F817, DHAR3) was consistently up-regulated at both time points in resistant genotypes, emphasizing its role in redox balance and ROS detoxification. In contrast, glyceraldehyde-3-phosphate dehydrogenase (K7UGF5, GAPC2), a glycolytic enzyme, was unique to the susceptible genotype, suggesting its involvement in managing energy metabolism under stress conditions. Our findings suggest that resistance to SLB in popcorn involves a combination of enhanced photosynthetic repair, redox homeostasis, and ribosomal protein activity, providing new potential molecular targets, such as DHAR3 and RPP0B, for genetic improvement in SLB resistance. These results offer valuable insights into breeding programs aimed at developing SLB-resistant popcorn varieties.
The Deubiquitinating Enzyme AMSH1 Contributes to Plant Immunity Through Regulating the Stability of BDA1
Authors: Wang Yiran , Huang Weijie , Li Xin , Zhang Yuelin
Published: February 2025

Plants utilize plasma membrane localized receptors like kinases (RLKs) or receptor-like proteins (RLPs) to recognize pathogens and activate pattern-triggered immunity (PTI) responses. A gain-of-function mutation in the Arabidopsis RLP SNC2 (SUPPRESSOR OF NPR1-1, CONSTITUTIVE 2) leads to constitutive activation of defense responses in snc2-1D mutant plants. Transcription factors, SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) and CALMODULIN-BINDING PROTEIN 60g (CBP60g), define two parallel pathways downstream of SNC2. The autoimmunity of snc2-1D was partially affected by single mutations in SARD1 or CBP60g but completely suppressed by the sard1 cbp60g double mutant. From a suppressor screen using sard1-1 snc2-1D, we identified a deubiquitinating enzyme ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM 1 (AMSH1) as a key component in SNC2-mediated plant immunity. A loss-of-function mutation in AMSH1 can suppress the autoimmune responses of sard1-1 snc2-1D. In eukaryotes, selective protein degradation often occurs through the ubiquitination/deubiquitination system. The deubiquitinating enzymes that remove ubiquitin from target proteins play essential roles in controlling the level of target protein ubiquitination and degradation. As loss of AMSH1 results in decreased BDA1 abundance and BDA1 is a transmembrane protein required for SNC2-mediated immunity, AMSH1 likely contributes to immunity regulation through controlling BDA1 stability.
Grain Weight and Taste Quality in Japonica Rice Are Regulated by Starch Synthesis and Grain Filling Under Nitrogen–Phosphorus Interactions
Authors: Jiang Hongfang , Zhao Yanze , Chen Liqiang , Wan Xue , Yan Bingchun , Liu Yuzhuo , Liu Yuqi , Zhang Wenzhong , Gao Jiping
Published: February 2025

To reveal the regulatory effects of nitrogen and phosphorus interactions on grain-filling- and starch-synthesis-related enzymes, and grain weight of superior grains (SGs) and inferior grains (IGs) and taste quality, the japonica rice cultivar Shennong 265 was grown under field conditions with three nitrogen levels (210, 178.5, and 147 kg N ha−1; N3, N2, and N1) and two phosphorus levels (105 and 73.5 kg P ha−1; P2 and P1). At the N3 level, the yield of P1 was significantly lower (by 19.26%) compared to P2; at the N2 and N1 levels, P1 yielded higher than P2, peaking at N2P1. Spikelets per panicle showed P2 exceeding P1 at the same nitrogen level, with the highest for both SGs and IGs observed at N2P2, followed by N2P1. Reductions in nitrogen and phosphorus decreased the grain-filling rate but prolonged the duration for grain-filling. N2P1 maintained grain weight by extending the grain-filling duration across the early, middle, and late stages of IGs, and the middle and late stages of SGs. Increased nitrogen enhanced the activities of soluble starch synthase (SSS) and starch branching enzyme (SBE), whereas increased phosphorus inhibited these activities in SGs but enhanced them in IGs. Reduced nitrogen and phosphorus fertilizer diminished ADP glucose pyrophosphorylase (AGPP) and granule-bound starch synthase (GBSS) activities in SGs and IGs, inhibiting amylose accumulation while enhancing taste value. Compared with N3P2, the taste value of N2P1 increased significantly by 6.93%, attributed to a higher amylopectin/amylose ratio. N2P1 (178.5 kg N ha−1 and 73.5 kg P ha−1) optimized enzyme activity, starch composition, and grain filling, balancing both yield and taste, and thus demonstrated an effective fertilization strategy for stable rice production.
Using Klebsiella sp. and Pseudomonas sp. to Study the Mechanism of Improving Maize Seedling Growth Under Saline Stress
Authors: Zhao Xiaoyu , Yu Xiaofang , Gao Julin , Qu Jiawei , Borjigin Qinggeer , Meng Tiantian , Li Dongbo
Published: February 2025

The increasing salinization of cultivated soil worldwide has led to a significant reduction in maize production. Using saline–alkaline-tolerant growth-promoting bacteria (PGPR) in the rhizosphere can significantly improve the saline tolerance of maize and ensure the stability of maize yields, which has become a global research hotspot. This study screened salt-tolerant microorganisms Klebsiella sp. (GF2) and Pseudomonas sp. (GF7) from saline soil to clarify the mechanism in improving the saline tolerance of maize. In this study, different application treatments (GF2, GF7, and GF2 + GF7) and no application (CK) were set up to explore the potential ecological relationships between the saline tolerance of maize seedlings, soil characteristics, and microorganisms. The results showed that co-occurrence network and Zi-Pi analysis identified Klebsiella and Pseudomonas as core microbial communities in the rhizosphere soil of maize seedlings grown in saline soil. The deterministic process of microbial assembly mainly controlled the bacterial community, whereas bacteria and fungi were governed by random processes. The application of saline–alkaline-resistant PGPR under saline stress significantly promoted maize seedling growth, increased the activity of soil growth-promoting enzymes, and enhanced total nitrogen, soil organic carbon, and microbial carbon and nitrogen contents. Additionally, it reduced soil salt and alkali ion concentrations [electrical conductivity (EC) and exchangeable Na+]. Among them, GF2 + GF7 treatment had the best effect, indicating that saline–alkaline-tolerant PGPR could directly or indirectly improve the saline tolerance of maize seedlings by improving the rhizosphere soil ecological environment. EC was the determining factor to promote maize seedling growth under saline–alkaline stress (5.56%; p < 0.01). The results provided an important theoretical reference that deciphers the role of soil factors and microecology in enhancing the saline tolerance of maize.
Factors Influencing Orchid Species Richness in the Central Balkans: The Importance of Belowground Organ Types
Authors: Djordjević Vladan , Lakušić Dmitar , Novković Ivan , Stevanović Vladimir , Tsiftsis Spyros
Published: February 2025

The Balkan Peninsula is considered one of the most important centres of orchid diversity in Europe. However, the patterns of orchid species richness in the Central Balkans have not been sufficiently studied so far. The aim of this study was, therefore, to identify the centres of orchid diversity and the factors that influence the spatial variation in orchid species richness in the Central Balkans. For the analyses, the area of the Central Balkans was divided into 10 × 10 km grid cells. The environmental variables determined for each grid cell and used in the analyses were altitude, bioclimatic variables, geological substrates and habitat types. A random forest (RF) analysis was used to identify the environmental predictors most strongly associated with species richness. In addition to the total number of taxa, orchids with three belowground organ types were analysed separately: (a) rhizomatous orchids, (b) orchids with palmately lobed and fusiform tubers (“palmate tuberous orchids”) and (c) orchids with spherical or ovoid tubers (“ovoid tuberous orchids”). In the Central Balkans, 54 orchid species and subspecies have been recorded, and the most important centres of diversity are the Tara, Zvijezda, Jadovnik and Zlatar Mountains and the Ovčar-Kablar Gorge. In general, two groups of grid cells with the largest number of orchid taxa, i.e., hotspots, stood out: (1) grid cells with a large altitudinal range and (2) grid cells occupied by gorges and ravines. The most important gradients influencing orchid species richness are specific habitat types and altitudinal ranges, while climatic factors and geological substrates are less important. The most important factors affecting the richness of total and rhizomatous orchids are altitudinal range and habitat types (Abieti-Fagenion, Ostryo-Carpinion orientalis and Pinion nigrae forests), highlighting the important role of habitat heterogeneity. The maximum altitude, percentage of Abieti-Fagenion and Vaccinio-Picetea forests and the minimum value of the mean temperature of the driest quarter are the most important factors for determining the richness of palmate tuberous orchids, whereas the percentage of xero-thermophilous habitat types (Ostryo-Carpinion orientalis, Asplenietea trichomanis and Pinion nigrae) has the greatest influence on the richness of ovoid tuberous orchids. These results confirm the hypothesis concerning the origin and development of underground organs in orchids, emphasising that palmate tuberous orchids are best adapted to cold and humid habitat conditions, whereas ovoid tuberous orchids have the ability to grow in habitats with very warm and dry conditions. This study provides a good basis for better orchid conservation planning and underlines the importance of belowground strategies as a feature of orchid life history that should be considered when studying patterns of orchid diversity.
The MADS-Box Transcription Factor CaRIN Positively Regulates Chlorophyll Degradation During Pepper (Capsicum annuum L.) Fruit Ripening by Repressing the Expression of CaLhcb-P4
Authors: Song Yingying , Cheng Qing , Li Xingzhe , Ma Shijie , Shen Huolin , Sun Liang
Published: February 2025

Pepper (Capsicum spp.) is an important global vegetable and spice, with fruit color being a key determinant of its commercial quality. However, the regulatory mechanisms underlying pepper fruit color are still not fully understood. This study focuses on the MADS-RIPENING INHIBITOR (MADS-RIN), a MADS-box transcription factor that regulates various aspects of fruit ripening, including pigmentation. We identified CaRIN, a homolog of tomato’s SlRIN, whose expression is closely associated with fruit ripening in pepper. Silencing CaRIN through virus-induced gene silencing (VIGS) resulted in increased chlorophyll and chlorophyll a content, reduced carotenoid accumulation, and uneven fruit coloration. Integrative analysis of the RNA-seq and DAP-seq data identified 77 target genes regulated by CaRIN, which was involved in processes such as chlorophyll metabolism and plant hormone signaling. Yeast one-hybrid (Y1H) and dual-luciferase (LUC) assays demonstrated that CaRIN directly bound to the promoter of CaLhcb-P4, repressing its expression. Downregulation of CaLhcb-P4 in pepper fruits via VIGS accelerated chlorophyll degradation. Additionally, CaRIN indirectly regulated multiple genes associated with chlorophyll and carotenoid metabolism, sugar transport, and cell wall degradation. These findings provide novel insights into the regulatory mechanisms of chlorophyll degradation during pepper fruit ripening, offering a foundation for further research and potential genetic improvement strategies.