bluuebunny/crossref_metadata_2025 huggingface dataset:
Perception of carbon capture and utilization - a framing analysis of German-speaking media
Authors: Fürst Kristina , Strunge Till
Published: September 2024

Carbon dioxide capture and utilization (CCU) technologies are one building block in Germany’s industrial decarbonization strategy. With CCU technologies, carbon dioxide emissions are captured from an industrial point source or the ambient air (direct air capture, DAC) and either used directly as an industrial feedstock or transformed and used as a carbon resource in industry. Despite the potential benefits of CCU in decreasing industrial dependency on fossil fuels and decreasing global CO2 emissions, robust empirical evidence of the general public opinion and societal acceptance of carbon capture and utilization technologies is lacking. Here, we studied the German-speaking media discourse as a proxy for the public discussion of carbon capture and utilization (CCU) technologies. We show that CCU technologies are overall framed more positively than negatively. Responsible for the optimistic framing are the two dominant media frames: “climate protection-frame” and “benefit-frame,” which are mainly used by scientists and policy actors or representatives from the industry sector respectively.
Thermodynamic analysis of a cascade organic Rankine cycle power generation system driven by hybrid geothermal energy and liquefied natural gas
Authors: Pan Zilin , Fu Yufei , Chen Hongwei , Song Yangfan
Published: September 2024

The combination of renewable energy and liquefied natural gas (LNG) cold energy can effectively improve energy utilization efficiency and achieve the goal of energy conservation and emission reduction, which is one of the important directions of future development. This work proposed a cascade organic Rankine cycle (ORC) driven by a geothermal heat source and an LNG heat sink. Seven organic fluids are chosen as candidates to form different working fluid pairs. The effects of the main design parameters on system performance are carried out through the thermodynamic analysis. Then, the optimal design conditions and fluid selection schemes are searched based on the single-objective optimization results. Finally, the exergy destruction study is conducted under the optimal design conditions and working fluid pair. Results showed that the cascade ORC system using the working fluid pair of R601/R290 had the highest exergy efficiency, which could reach 20.02%. At the same time, under the optimal design conditions, the secondary cycle condenser and LNG direct expansion brought high exergy destruction, which was respectively 29.3% and 25.8%, and followed by the two turbines in the cascade ORC system, which were 16.1%, 11.2% and 7.7%.
Assessing the potential of diverse pastures for reducing nitrogen leaching
Authors: Graham Scott L. , Pronger Jack , Laubach Johannes , Hunt John E. , Rogers Graeme N. D. , Carrick Sam , Whitehead David , McLeod Malcolm , Mitchell Grace , Mudge Paul
Published: September 2024

Mitigation practices for nitrogen leaching losses from livestock agriculture are needed to protect freshwater quality and increase the efficiency of agricultural production. Within New Zealand, the most common pasture type is a two-species mix of perennial ryegrass (Lolium perenne) and white clover (Trifolium repens). Ecological theory suggests that increasing species and functional diversity improves ecosystem function, including nitrogen (N) retention. Use of more diverse pasture types, including a mix of pasture grasses, legumes and other forbs, particularly plantain (Plantago lanceolata), with functional traits, including winter activity, deep-rooting, N fixation, and biological inhibition of nitrification in the soil, is a potential mitigation practice that requires further verification with long-term field measurements. Here we utilize a network of large lysimeters to make field-based measurements of N leaching from 5–8 species diverse pasture, including plantain, under a range of soil, climate and management conditions, for comparison with losses from traditional ryegrass-clover pasture. Over 3 years of measurements, leaching from fully established diverse pasture was 2–80 kg N ha−1 y−1. No differences were observed in dry matter production or N leaching of diverse pasture compared to ryegrass-clover lysimeters. Large losses, up to 120 kg N ha−1, were observed during periods when pasture was not fully established, including cultivation and sowing of new pasture, depending on season. Timing of management activities could be optimized to minimize these losses. These data provide critical assessment of diverse pasture as a mitigation approach for reducing N losses. Further work on diverse pastures should include higher diversity mixes as well as consideration of animal mediated effects of diverse pasture diets on N inputs.
Co-regulation of innate and adaptive immune responses induced by ID93+GLA-SE vaccination in humans
Authors: Fiore-Gartland Andrew , Srivastava Himangi , Seese Aaron , Day Tracey , Penn-Nicholson Adam , Luabeya Angelique Kany Kany , Du Plessis Nelita , Loxton Andre G. , Bekker Linda-Gail , Diacon Andreas , Walzl Gerhard , Sagawa Zachary K. , Reed Steven G. , Scriba Thomas J. , Hatherill Mark , Coler Rhea
Published: September 2024

Introduction

Development of an effective vaccine against tuberculosis is a critical step towards reducing the global burden of disease. A therapeutic vaccine might also reduce the high rate of TB recurrence and help address the challenges of drug-resistant strains. ID93+GLA-SE is a candidate subunit vaccine that will soon be evaluated in a phase 2b efficacy trial for prevention of recurrent TB among patients undergoing TB treatment. ID93+GLA-SE vaccination was shown to elicit robust CD4+ T cell and IgG antibody responses among recently treated TB patients in the TBVPX-203 Phase 2a study (NCT02465216), but the mechanisms underlying these responses are not well understood.

Methods

In this study we used specimens from TBVPX-203 participants to describe the changes in peripheral blood gene expression that occur after ID93+GLA-SE vaccination.

Results

Analyses revealed several distinct modules of co-varying genes that were either up- or down-regulated after vaccination, including genes associated with innate immune pathways at 3 days post-vaccination and genes associated with lymphocyte expansion and B cell activation at 7 days post-vaccination. Notably, the regulation of these gene modules was affected by the dose schedule and by participant sex, and early innate gene signatures were correlated with the ID93-specific CD4+ T cell response.

Discussion

The results provide insight into the complex interplay of the innate and adaptive arms of the immune system in developing responses to vaccination with ID93+GLA-SE and demonstrate how dosing and schedule can affect vaccine responses.
Trends and research foci in immunoregulatory mechanisms of allergic rhinitis: a bibliometric analysis (2014-2024)
Authors: Wang Yandan , Zhang Liangran , Shi Baoyuan , Luo Junpeng
Published: September 2024

Background

This study aims to provide a comprehensive bibliometric analysis of research trends, hotspots, and future directions in the immunoregulatory mechanisms of allergic rhinitis (AR) from 2014 to 2024.

Methods

Data were sourced from the Web of Science Core Collection (WoSCC), covering articles and reviews published between April 1, 2014, and March 31, 2024. The search terms included “Allergic Rhinitis,” “AR,” and related terms along with specific keywords related to immune cells and inflammatory mediators. Bibliometric tools such as CiteSpace, VOSviewer, and SCImago Graphica were used to analyze institutional cooperation networks, keyword co-occurrence, citation bursts, and research topic evolution. Microsoft Excel 2019 was employed to display annual publication trends.

Results

A total of 2200 papers met the inclusion and exclusion criteria. The number of publications showed an upward trend over the past decade, with a significant peak in 2021. China (583 papers) and the United States (454 papers) were the major contributing countries. Imperial College London emerged as the leading institution. Key research frontiers identified include the roles of NF kappa B and air pollution in AR. Keyword burst analysis revealed emerging topics such as respiratory allergy and personalized treatment strategies. Notable limitations include the exclusive use of the WoSCC database and the restriction to English-language publications.

Conclusion

The field of immunoregulatory mechanisms in allergic rhinitis has seen significant growth, with China and the United States leading the research. Future research should focus on developing personalized treatment plans and understanding the comprehensive impact of environmental factors. Continued interdisciplinary collaboration and international cooperation will be essential for advancing therapeutic strategies in AR.
Silicon balance in an integrated multi-tropical aquaculture ecosystem, Sanggou Bay, China
Authors: Li Ruihuan , Jiang Zengjie , Lin Fan , Zhang Yitao , Wang Weixin , Zhang Kai , Li Weiwei , Wan Dongjie , Shi Yazhou
Published: September 2024

Farmed aquaculture species play an important role in regulating nutrient cycles in farming systems. Compared with nitrogen and phosphorus, the role of farmed species in the silicon (Si) cycle remains poorly understood. To help reduce this uncertainty, we clarified the sources and sinks of silicate and quantified the Si pools in an aquaculture system in Sanggou Bay (SGB). The results showed that dissolved inorganic nutrient levels were significantly lower during the dry season than during the wet. Dissolved silicate (DSi) is a potential limiting factor for phytoplankton growth during spring, and phosphorus limitation occurs during summer. The budget results indicated that large amounts of nitrogen, phosphate (DIP), and DSi were buried in the sediment or transformed into other forms during both the wet and dry seasons. The nitrogen and DIP cycles were strongly influenced by bivalve excretion and farmed species harvesting; however, these processes had little impact on the Si cycle. Si availability depends on both external inputs and internal recycling. DSi was primarily supplied from the Yellow Sea, with a minor contribution from the river due to river discharge during spring. However, during summer, riverine inflow (accounting for 83% of the total influx) was the major DSi source followed by benthic flux (12%). Biogenic silica (BSi) burial efficiency in the sediment was estimated to be 78% during spring and 23% during summer. The BSi preservation efficiency in bivalves during spring was high (53%), leading to a higher Si retention than in river discharge. Bivalves biodeposition plays an important role in the Si burial process. We suggest that this high retention is essentially controlled by the biodeposition mechanism, which is directly controlled by the exotic suspension feeders. Bivalves have the potential to alter Si retention in the bay by producing large amounts of biodeposits and accelerating the silica cycle, which may lead to more carbon dioxide being absorbed by diatoms.
Regional diversity in drug-induced lung diseases among the USA, European Union, and Japan
Authors: Sato Jun , Sadachi Ryo , Koyama Takafumi , Katsuya Yuki , Okada Mao , Yamamoto Noboru
Published: September 2024

Background

Drug-induced lung disease (DILD) is a considerable and potentially fatal adverse event with poorly understood risk factors. Large-scale, data-driven analyses investigating regional discrepancies in DILD incidence are lacking. The aim of this study was to investigate the potential association among DILD prevalence, regional differences and other factors based on large-scale data base.

Methods

This retrospective observational study analyzed spontaneous adverse event reports from the FDA Adverse Event Reporting System (FAERS) database between January 2010 and December 2020. Regional disparities in DILD incidence were assessed among reports from the United States of America (USA), the European Union (EU), and Japan (JP). Using multivariate logistic regression accounting for age, sex, and reporting years, we calculated the reporting odds ratios (RORs) with 95% confidence intervals. Subgroup analyses were performed for different types of anticancer agents, including tyrosine kinase inhibitors (TKIs), immune checkpoint inhibitors (ICIs), antibody–drug conjugates (ADCs), and cytotoxic agents.

Results

Regional differences in RORs were observed for anticancer drugs in reports from JP and the EU compared with those from the USA (JP, ROR 4.432; EU, ROR 1.291) and for non-anticancer drugs (JP, ROR 3.481; EU, ROR 1.086). Significantly higher RORs were observed for all anticancer drug regimens reported in JP than in the USA (TKIs, ROR 3.274; ICIs, ROR 2.170; ADCs, ROR 2.335; cytotoxic agents, ROR 3.989). The EU reports exhibited higher RORs for TKIs and cytotoxic agents than the USA reports, with no significant differences in ICIs or ADCs (TKIs, ROR 1.679; ICIs, ROR 1.041; ADCs, ROR 1.046; cytotoxic agents, ROR 1.418).

Conclusion

The prevalence of DILD in JP, the EU, and the USA differed. These findings have important implications in evaluating the safety profiles of drugs and patient safety in drug development and clinical practice. This study is the first to identify regional differences in DILDs using a large global database.
Unveiling the microbial diversity across the northern Ninety East Ridge in the Indian Ocean
Authors: Li Ding , Wang Liping , Jiang Fan , Zeng Xiang , Xu Qinzeng , Zhang Xuelei , Zheng Qiang , Shao Zongze
Published: September 2024

Prokaryotes play a crucial role in marine ecosystem health and drive biogeochemical processes. The northern Ninety East Ridge (NER) of the Indian Ocean, a pivotal yet understudied area for these cycles, has been the focus of our study. We employed high-throughput 16S rRNA gene sequencing to analyze 35 water samples from five stations along the ridge, categorized into three depth- and dissolved oxygen-level-based groups. Our approach uncovered a clear stratification of microbial communities, with key bioindicators such as Prochlorococcus MIT9313, Sva0996 marine group, and Candidatus Actinomarina in the upper layer; Ketobacter, Pseudophaeobacter, Nitrospina, and SAR324 clade in the middle layer; and Methylobacterium-Methylorubrum, Sphingomonas, Sphingobium, and Erythrobacter in the deep layer. Methylobacterium-Methylorubrum emerged as the most abundant bacterial genus, while Nitrosopumilaceae predominated among archaeal communities. The spatial and depth-wise distribution patterns revealed that Ketobacter was unique to the northern NER, whereas Methylobacterium-Methylorubrum, UBA10353, SAR324 clade, SAR406, Sva0996_marine_group, Candidatus Actinomarina were ubiquitous across various marine regions, exhibiting niche differentiation at the OTU level. Environmental factors, especially dissolved oxygen (DO), silicate, nitrate, and salinity, significantly influence community structure. These findings not only reveal the novelty and adaptability of the microbial ecosystem in the northern NER but also contribute to the broader understanding of marine microbial diversity and its response to environmental heterogeneity.
Human in the collaborative loop: a strategy for integrating human activity recognition and non-invasive brain-machine interfaces to control collaborative robots
Authors: Pilacinski Artur , Christ Lukas , Boshoff Marius , Iossifidis Ioannis , Adler Patrick , Miro Michael , Kuhlenkötter Bernd , Klaes Christian
Published: September 2024

Human activity recognition (HAR) and brain-machine interface (BMI) are two emerging technologies that can enhance human-robot collaboration (HRC) in domains such as industry or healthcare. HAR uses sensors or cameras to capture and analyze the movements and actions of humans, while BMI uses human brain signals to decode action intentions. Both technologies face challenges impacting accuracy, reliability, and usability. In this article, we review the state-of-the-art techniques and methods for HAR and BMI and highlight their strengths and limitations. We then propose a hybrid framework that fuses HAR and BMI data, which can integrate the complementary information from the brain and body motion signals and improve the performance of human state decoding. We also discuss our hybrid method’s potential benefits and implications for HRC.
Activation of arginine vasopressin receptor 1a reduces inhibitory synaptic currents at reciprocal synapses in the mouse accessory olfactory bulb
Authors: Taniguchi Mutsuo , Murata Yoshihiro , Yamaguchi Masahiro , Kaba Hideto
Published: September 2024

Central arginine vasopressin (AVP) facilitates social recognition and modulates many complex social behaviors in mammals that, in many cases, recognize each other based on olfactory and/or pheromonal signals. AVP neurons are present in the accessory olfactory bulb (AOB), which is the first relay in the vomeronasal system and has been demonstrated to be a critical site for mating-induced mate recognition (olfactory memory) in female mice. The transmission of information from the AOB to higher centers is controlled by the dendrodendritic recurrent inhibition, i.e., inhibitory postsynaptic currents (IPSCs) generated in mitral cells by recurrent dendrodendritic inhibitory inputs from granule cells. These reports suggest that AVP might play an important role in regulating dendrodendritic inhibition in the AOB. To test this hypothesis, we examined the effects of extracellularly applied AVP on synaptic responses measured from mitral and granule cells in slice preparations from 23-–36-day-old Balb/c mice. To evoke dendrodendritic inhibition in a mitral cell, depolarizing voltages of −70 to 0 mV (10 ms duration) were applied to a mitral cell using a conventional whole-cell configuration. We found that AVP significantly reduced the IPSCs. The suppressive effects of AVP on the IPSCs was diminished by an antagonist for vasopressin receptor 1a (V1aR) (Manning compound), but not by an antagonist for vasopressin receptor 1b (SSR149415). An agonist for V1aRs [(Phe2)OVT] mimicked the action of AVP on IPSCs. Additionally, AVP significantly suppressed voltage-activated currents in granule cells without affecting the magnitude of the response of mitral cells to gamma-aminobutyric acid (GABA). The present results suggest that V1aRs play a role in reciprocal transmission between mitral cells and granule cells in the mouse AOB by reducing GABAergic transmission through a presynaptic mechanism in granule cells.
Junior athletes’ nutritional demands: a narrative review of consumption and prevalence of eating disorders
Authors: Amawi Adam , Khataybeh Batool , Al Aqaili Raghad , Ababneh Nour , Alnimer Lana , Qoqazeh Ali , Oukal Farah , Jahrami Haitham , Mousa Ay Khitam , Al Saoud Hassan , Ghazzawi Hadeel
Published: September 2024

Information regarding the dietary requirements and consumption of young athletes is limited. Hence, the aim of this narrative review is to provide a comprehensive combination of research and review papers on the nutritional status of young athletes aged 5–18 years old, as well as quantitative, qualitative, wholesome foods, food choices, and eating disordered data concerning the dietary requirements for growing young athletes. This study involved systematic searches of electronic databases, including Google Scholar, PubMed, Science Direct, Scopus, and Web of Science. The specific criteria for identifying research papers published in English from July 1980 until May 2024 were included. Only 48 studies out of 1,262 were included in this narrative review. The findings of this study suggest that, compared with adults, junior athletes need a unique approach to meet their dietary needs. Growth, development, and general athletic performance depend on macronutrients, as they are vital nutrients for young active athletes. However, research on enhancing junior athletes’ performance is still in progress, and studies on hydration status, and eating disorders are limited.
Reduction in Lp(a) after a medically supervised, prolonged water-only fast followed by a whole-plant-food diet free of added salt, oil, and sugar: a case report
Authors: Thompson Natasha , Streutker Anthony , Goldhamer Alan C. , Myers Toshia R.
Published: September 2024

Lipoprotein(a) [Lp(a)] is a low-density lipoprotein (LDL) associated with increased cardiovascular disease (CVD) risk. High Lp(a) levels are genetically determined and lack effective pharmacotherapy. This case report describes a 67-year-old, vegan male with elevated blood pressure (BP), total cholesterol (TC), LDL, and Lp(a) who underwent a 10-day, medically supervised water-only fast followed by a 6-week SOS-free diet (free of added salt, oil, and sugar). At the 6-week-follow-up visit, he experienced significant reductions in several CVD risk markers, including blood pressure, total cholesterol, LDL, and high-sensitivity C-reactive protein. He also experienced an unexpected decrease in Lp(a), from 236.3 nmol/L to 143.4 nmol/L (39%). This decrease is comparable to reductions achieved with proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors. These findings suggest that prolonged water-only fasting and/or an SOS-free diet may be an effective alternative approach for managing high Lp(a) levels and reducing CVD risk in a vegan population, warranting further research.
Radiomics in distinguishing between lung adenocarcinoma and lung squamous cell carcinoma: a systematic review and meta-analysis
Authors: Shi Lili , Zhao Jinli , Wei Zhichao , Wu Huiqun , Sheng Meihong
Published: September 2024

Objectives

The aim of this study was to systematically review the studies on radiomics models in distinguishing between lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) and evaluate the classification performance of radiomics models using images from various imaging techniques.

Materials and methods

PubMed, Embase and Web of Science Core Collection were utilized to search for radiomics studies that differentiate between LUAD and LUSC. The assessment of the quality of studies included utilized the improved Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) and Radiomics Quality Score (RQS). Meta-analysis was conducted to assess the classification performance of radiomics models using various imaging techniques.

Results

The qualitative analysis included 40 studies, while the quantitative synthesis included 21 studies. Median RQS for 40 studies was 12 (range -5~19). Sixteen studies were deemed to have a low risk of bias and low concerns regarding applicability. The radiomics model based on CT images had a pooled sensitivity of 0.78 (95%CI: 0.71~0.83), specificity of 0.85 (95%CI:0.73~0.92), and the area under summary receiver operating characteristic curve (SROC-AUC) of 0.86 (95%CI:0.82~0.89). As for PET images, the pooled sensitivity was 0.80 (95%CI: 0.61~0.91), specificity was 0.77 (95%CI: 0.60~0.88), and the SROC-AUC was 0.85 (95%CI: 0.82~0.88). PET/CT images had a pooled sensitivity of 0.87 (95%CI: 0.72~0.94), specificity of 0.88 (95%CI: 0.80~0.93), and an SROC-AUC of 0.93 (95%CI: 0.91~0.95). MRI images had a pooled sensitivity of 0.73 (95%CI: 0.61~0.82), specificity of 0.80 (95%CI: 0.65~0.90), and an SROC-AUC of 0.79 (95%CI: 0.75~0.82).

Conclusion

Radiomics models demonstrate potential in distinguishing between LUAD and LUSC. Nevertheless, it is crucial to conduct a well-designed and powered prospective radiomics studies to establish their credibility in clinical application.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=412851, identifier CRD42023412851.
A complete benchmark for polyp detection, segmentation and classification in colonoscopy images
Authors: Tudela Yael , Majó Mireia , de la Fuente Neil , Galdran Adrian , Krenzer Adrian , Puppe Frank , Yamlahi Amine , Tran Thuy Nuong , Matuszewski Bogdan J. , Fitzgerald Kerr , Bian Cheng , Pan Junwen , Liu Shijle , Fernández-Esparrach Gloria , Histace Aymeric , Bernal Jorge
Published: September 2024

Introduction

Colorectal cancer (CRC) is one of the main causes of deaths worldwide. Early detection and diagnosis of its precursor lesion, the polyp, is key to reduce its mortality and to improve procedure efficiency. During the last two decades, several computational methods have been proposed to assist clinicians in detection, segmentation and classification tasks but the lack of a common public validation framework makes it difficult to determine which of them is ready to be deployed in the exploration room.

Methods

This study presents a complete validation framework and we compare several methodologies for each of the polyp characterization tasks.

Results

Results show that the majority of the approaches are able to provide good performance for the detection and segmentation task, but that there is room for improvement regarding polyp classification.

Discussion

While studied show promising results in the assistance of polyp detection and segmentation tasks, further research should be done in classification task to obtain reliable results to assist the clinicians during the procedure. The presented framework provides a standarized method for evaluating and comparing different approaches, which could facilitate the identification of clinically prepared assisting methods.
Epidemiological investigation of allergic rhinitis in children aged 6–12 years in Bayannur City, China
Authors: Yan Xiaobo , Li Limin
Published: September 2024

Background

Allergic rhinitis (AR) is an inflammatory condition of the nasal mucosa triggered by exposure to non-harmful substances. Over the past decade, the prevalence of AR in Chinese children has been steadily increasing. However, detailed epidemiological data on AR in children from Bayannur City are lacking.

Methods

This study randomly selected six primary schools in Bayannur City. Electronic questionnaires were distributed via the web, and parents and children completed the questionnaires by scanning the two-dimensional code within a designated timeframe. Statistical analysis was performed on the collected data.

Results

A total of 4,754 valid responses were obtained. The self-reported prevalence of AR among children in Bayannur city was 39.79%. Multivariate analysis revealed that male gender, belonging to an ethnic minority, a history of food or drug allergies, frequent antibiotic use (≥3 times per year in the past two years, with each course lasting ≥3 days), and residence in urban or pastoral areas was associated with an increased prevalence of AR in children. The proportion of children experiencing moderate to severe AR hat impacted their studies or daily life was 48.78%. Chronic AR was reported in 56.71% of cases. Among AR patients with other allergic conditions, the incidence rates were as follows: bronchial asthma 35.99%, upper airway cough syndrome (UACS) 64.32%, secretory otitis media (SOM) 22.41%, obstructive sleep apnea hypopnea-syndrome (OSAHS) 49.58%, allergic dermatitis (AD) 48.72%, and allergic conjunctivitis (AC) 85.20%. The prevalence of AR was 50.30% in urban areas, 13.733% in rural areas and 20.90% in pastoral areas. Seasonal effects on AR prevalence were notably significant in urban and pastoral regions.

Conclusions

The prevalence of AR among children in Bayannur city was 39.80%. Of those with AR, 48.72% experienced significant impacts on their learning or daily life, while only 14.80% had no other allergic conditions. There were significant variations in the prevalence and onset of AR among children between urban, agricultural and pastoral areas.
Case Report: Primary ciliary dyskinesia due to CCNO mutations: a Chinese pediatric case series and literature review
Authors: Tong Lejun , Li Li , Wang Wenjian , Chen Jiehua
Published: September 2024

Primary ciliary dyskinesia (PCD) is a hereditary disorder characterized by defects in cilia that impair mucociliary clearance. This study focuses on PCD caused by mutations in the Cyclin O (CCNO) gene and reports on three cases involving Chinese children. Case 1 was an 8-year-and-3-month-old boy who presented with respiratory distress after birth and later developed a recurrent productive cough and purulent nasal discharge. He was initially diagnosed with diffuse panbronchiolitis (DPB) due to the presence of diffuse micronodules in lung CT scans. Case 2 was the younger sister of case 1. She also presented with respiratory distress after birth, with a chest radiograph revealing atelectasis. She required oxygen supplementation until the age of 2 months. Case 3 was a 4-year-and-4-month-old girl with a history of neonatal pneumonia, persistent pulmonary atelectasis, and recurrent lower respiratory tract infections. Her chest radiograph also showed diffuse micronodules. In all three cases, the final diagnosis of PCD was confirmed by genetic testing. Cases 1 and 2 exhibited homozygous c.248_252dup TGCCC (p.G85Cfs*11) mutations in the CCNO gene, while case 3 harbored a homozygous c.258_262dup GGCCC (p.Q88Rfs*8) mutation. A literature review indicated that the common clinical features of CCNO-PCD include neonatal respiratory distress (40/49, 81.6%), chronic cough (31/33, 93.9%), rhinosinusitis (30/35, 85.7%), bronchiectasis (26/35, 74.3%), and low nasal nitric oxide (nNO, 40/43, 93.0%). Notably, situs inversus has not been reported. In CCNO-PCD patients, cilia may appear structurally normal but were severely reduced in number or entirely absent. Lung CT scans in these patients may exhibit diffuse micronodules and “tree-in-bud” signs, which can lead to a clinical misdiagnosis of DPB. nNO screening combined with genetic testing is an optimized diagnostic strategy. Treatment options include the use of anti-infective and anti-inflammatory agent, along with daily airway clearance. This study underscores the importance of genetic testing in neonates and children with suspected PCD or those clinically diagnosed with DPB to enable an early diagnosis and prompt intervention, thereby enhancing the prognosis for these patients.
Potential mechanisms underlying podophyllotoxin-induced cardiotoxicity in male rats: toxicological evidence chain (TEC) concept
Authors: Ma Kaiyue , Sun Lu , Jia Chunxue , Kui Hongqian , Xie Jiaqi , Zang Shidan , Huang Shixin , Que Jinfeng , Liu Chuanxin , Huang Jianmei
Published: September 2024

Introduction

Podophyllotoxin (PPT) is a high-content and high-activity compound extracted from the traditional Chinese medicinal plant Dysosma versipellis (DV) which exhibits various biological activities. However, its severe toxicity limits its use. In clinical settings, patients with DV poisoning often experience adverse reactions when taking large doses in a short period. The heart is an important toxic target organ, so it is necessary to conduct 24-h acute cardiac toxicity studies on PPT to understand its underlying toxicity mechanism.

Methods

Based on the concept of the toxicological evidence chain (TEC), we utilized targeted metabolomic and transcriptomic analyses to reveal the mechanism of the acute cardiotoxicity of PPT. The manifestation of toxicity in Sprague-Dawley rats, including changes in weight and behavior, served as Injury Phenotype Evidence (IPE). To determine Adverse Outcomes Evidence (AOE), the hearts of the rats were evaluated through histopathological examination and by measuring myocardial enzyme and cardiac injury markers levels. Additionally, transcriptome analysis, metabolome analysis, myocardial enzymes, and cardiac injury markers were integrated to obtain Toxic Event Evidence (TEE) using correlation analysis.

Results

The experiment showed significant epistaxis, hypokinesia, and hunched posture in PPT group rats within 24 h after exposure to 120 mg/kg PPT. It is found that PPT induced cardiac injury in rats within 24 h, as evidenced by increased serum myocardial enzyme levels, elevated concentrations of cardiac injury biomarkers, and altered cardiac cell morphology, all indicating some degree of cardiac toxicity. Transcriptome analysis revealed that primary altered metabolic pathway was arachidonic acid metabolism after PPT exposure. Cyp2e1, Aldob were positively correlated with differential metabolites, while DHA showed positive correlation with differential genes Fmo2 and Timd2, as well as with heart injury markers BNP and Mb.

Conclusion

This study comprehensively evaluated cardiac toxicity of PPT and initially revealed the mechanism of PPT-induced acute cardiotoxicity, which involved oxidative stress, apoptosis, inflammatory response, and energy metabolism disorder.
Mining and evaluation of adverse event signals for capmatinib based on the FAERS database
Authors: Chen Xinnan , Jiang Ying , Zhu Haohao , Tian Man
Published: September 2024

Objective

To conduct a comprehensive data analysis based on the FDA’s Adverse Event Reporting System (FAERS) to mine possible adverse event (AE) signals of Capmatinib, providing valuable references for its clinical application.

Methods

Capmatinib was the primary suspected drug in the search of FAERS database from the second quarter of 2020 to the fourth quarter of 2023. Data processing, screening, and classification were performed using methods such as the Reporting Odds Ratio (ROR), Proportional Reporting Ratio (PRR), Bayesian Confidence Propagation Neural Network (BCPNN), and Multi-item Gamma Poisson Shrinker (MGPS).

Results

A total of 1,991 AE reports directly related to Capmatinib were screened, identifying 269 Preferred Terms (PTs) involving 26 System Organ Classes (SOCs). Besides the AEs recorded in the drug label (such as edema, nausea, fatigue, and dyspnea), the study unearthed other high-risk AEs not listed in the label, including Renal and urinary disorders, Vocal cord paralysis, and Ear and labyrinth disorders. Among these, renal and urinary disorders, and ear and labyrinth disorders had a higher frequency and intensity of signals, suggesting that their mechanisms of occurrence could be a future research direction.

Conclusion

This study uncovered new potential AEs of Capmatinib based on the FAERS database, providing reference for its safe clinical use. Special attention should be given to the occurrence of ear and labyrinth disorders and renal and urinary disorders, primarily presenting as pseudo-acute kidney injury, during treatment.
Population pharmacokinetic study in children with vascular anomalies: body weight as a key variable in predicting the initial dose and dosing frequency of sirolimus
Authors: Fan Lin , Guo Hong-Li , Zhao Yue-Tao , Li Yue , Wang Wei-Jun , Huang Jian , Hu Ya-Hui , Zou Ji-Jun , Chen Feng
Published: September 2024

Background

The main challenges faced when using sirolimus in children with vascular anomalies (VAs) still include significant pharmacokinetic (PK) variability, uncertainty in the target concentration range, as well as inconsistencies in initial dosing and dosing frequency. The aim of this study is to establish a new population pharmacokinetic (PPK) model for children with VAs to guide the individualized use of sirolimus.

Methods

A PPK study was performed using data from children with VAs who received sirolimus between July 2017 and April 2022. A nonlinear mixed-effect modeling with a one-compartment model structure was applied. Monte Carlo simulation was employed to propose specific dosing recommendations to achieve the target trough concentrations (Ctrough) of 5–15 ng/mL.

Results

In total, 134 blood concentrations from 49 pediatric patients were used to characterize the sirolimus pharmacokinetics. Covariate analysis identified body weight (BW) as a significant factor affecting clearance (CL) in the final PPK model. The typical clearance rate and distribution volume, standardized to a BW of 16 kg, were 4.06 L/h (4% relative standard error, RSE) and 155 L (26% RSE), respectively. Optimal dosing regimens were simulated for different BWs. For a twice-daily regimen, the recommended doses were 0.05, 0.06, 0.07, and 0.08 mg/kg/day for BW of <10, 10–20, 20–40, and ≥40 kg, respectively; for a once-daily regimen, the recommended doses were 0.06, 0.07, 0.08, and 0.09 mg/kg/day for BW of <10, 10–30, 30–50, and ≥50 kg, respectively. Notably, sirolimus Ctrough could be maintained between 5–15 ng/mL across various dosing frequencies based on the recommended dosing regimen.

Conclusion

We established a PPK model of sirolimus for children with VAs and proposed an initial dosing strategy. Integrating initial dose and medication frequency recommendations into sirolimus’ guidelines will broaden its clinical options and simplify the clinical management for childhood VAs.
Edible cannabis for chronic low back pain: associations with pain, mood, and intoxication
Authors: Melendez Samantha N. , Ortiz Torres Marco , Lisano Jonathan K. , Giordano Gregory , Skrzynski Carillon , Hutchison Kent E. , Bryan Angela D. , Bidwell L. Cinnamon
Published: September 2024

Introduction

Cannabis, commonly known for both therapeutic and intoxicating effects, is gaining accessibility on legal markets and traction as a potential alternative therapy for pain mediation, particularly in those suffering from chronic low back pain. However, the effectiveness in this population of legal market forms of cannabis, particularly commonly used edibles, is unknown.

Methods

Therefore, this study utilized a naturalistic prospective design where participants with chronic low back pain with intentions to initiate cannabis use for treatment were recruited and self-selected edible cannabis products containing varying amounts of delta- 9 tetrahydrocannabinol (THC) and cannabidiol (CBD). Products were categorized as CBD-dominant, THC-dominant, or combined THC and CBD (THC + CBD).

Results

249 participants [140 female (56.62%), mean (SD) age of 46.30 (16.02), 90% White] were tracked over 2 weeks of ad libitum use and assessed during a naturalistic acute cannabis administration session on changes in pain, mood, and subjective drug effects. During acute administration, a significant correlation between THC dose and short-term pain relief was found, suggesting that higher THC doses were associated with greater pain reduction (p < .05). In addition, THC was associated with higher levels of subjective cannabis drug effects (p < .001), regardless of whether CBD was also in the edible product. Acute CBD dose was primarily associated with short-term tension relief (p < .05); however, there were no associations between CBD dose and acute pain. Over the 2-week ad libitum administration period results suggested pain reductions across participants using all forms of cannabis. However, trends suggested that more frequent use of CBD-dominant edible cannabis may be associated with greater reductions in perceived pain over the 2-week observation period (p = .07).

Discussion

These findings support the short-term analgesic effects of THC and anxiolytic effects of CBD and further suggest that orally-administered THC and CBD should continue to be evaluated for the potential to provide both acute and extended relief from chronic low back pain.

Clinical Trial Registration:

https://clinicaltrials.gov/study/NCT03522324?locStr=Boulder,%20CO&country=United%20States&state=Colorado&city=Boulder&cond=chronic%20low%20back%20pain&intr=Cannabis&rank=1, identifier NCT03522324.
Machine learning approaches for improving atomic force microscopy instrumentation and data analytics
Authors: Masud Nabila , Rade Jaydeep , Hasib Md. Hasibul Hasan , Krishnamurthy Adarsh , Sarkar Anwesha
Published: September 2024

Atomic force microscopy (AFM) is a part of the scanning probe microscopy family. It provides a platform for high-resolution topographical imaging, surface analysis as well as nanomechanical property mapping for stiff and soft samples (live cells, proteins, and other biomolecules). AFM is also crucial for measuring single-molecule interaction forces and important parameters of binding dynamics for receptor-ligand interactions or protein-protein interactions on live cells. However, performing AFM measurements and the associated data analytics are tedious, laborious experimental procedures requiring specific skill sets and continuous user supervision. Significant progress has been made recently in artificial intelligence (AI) and deep learning (DL), extending into microscopy. In this review, we summarize how researchers have implemented machine learning approaches so far to improve the performance of atomic force microscopy (AFM), make AFM data analytics faster, and make data measurement procedures high-throughput. We also shed some light on the different application areas of AFM that have significantly benefited from applications of machine learning frameworks and discuss the scope and future possibilities of these crucial approaches.
LiDAR point cloud simplification strategy utilizing probabilistic membership
Authors: Hu Ao , Xu Kaijie , Yin Xukun , Wang Di
Published: September 2024

With the continuous progress of information acquisition technology, the volume of LiDAR point cloud data is also expanding rapidly, which greatly hinders the subsequent point cloud processing and engineering applications. In this study, we propose a point cloud simplification strategy utilizing probabilistic membership to address this challenge. The methodology initially develops a feature extraction scheme based on curvature to identify the set of feature points. Subsequently, a combination of k-means clustering and Possibilistic C-Means is employed to partition the point cloud into subsets, and to simultaneously acquire the probabilistic membership information of the point cloud. This information is then utilized to establish a rational and efficient simplification scheme. Finally, the simplification results of the feature point set and the remaining point set are merged to obtain the ultimate simplification outcome. This simplification method not only effectively preserves the features of the point cloud while maintaining uniformity in the simplified results but also offers flexibility in balancing feature retention and the degree of simplification. Through comprehensive comparative analysis across multiple point cloud models and benchmarking against various simplification methods, the proposed approach demonstrates superior performance. Finally, the proposed algorithm was critically discussed in light of the experimental results.
An integrated targeted metabolome of phytohormones and transcriptomics analysis provides insight into the new generation of crops: Polygonatum kingianum var. grandifolium and Polygonatum kingianum
Authors: Ning Luyun , Xiao Qian , Tan Chensi , Gong Limin , Liu Yeman , Wang Zhi , He Shujin , He Chengdong , Yuan Hanwen , Wang Wei
Published: September 2024

Huangjing is becoming a new generation of crop. Polygonatum kingianum var. grandifolium (XHJ) is a variant of P. kingianum (DHJ), and they are treated as Huangjing. Unlike other Polygonatum species, the rhizome bud of XHJ can germinate both in spring and autumn, which contributes to its high rhizome yield. However, the molecular mechanism of the autumn shooting of XHJ was still unknown. In the present study, cellular observation, comparative targeted metabolome of phytohormones, and transcriptome analysis between XHJ and DHJ in autumn were conducted. Interestingly, ‘Diterpenoid biosynthesis’ (ko00904) and ‘Plant hormone signal transduction’ (ko04075) were commonly enriched by differentially accumulated phytohormones (DAPs) and differentially expressed genes (DEGs) in all tissues, which indicated the high auxin content, low cytokinin (CTK) content, and low abscisic acid/gibberellin (ABA/GA) ratio might contribute to the XHJ rhizome buds’ differentiation and germination in autumn. Moreover, according to the weighted gene co-expression network analysis (WCGNA), transcript factors (TFs) related to auxin, CTK, GA, and jasmonic acid (JA) metabolism were screened, such as AP2/ERFs, WRKY, and NAC, which deserve further research. In conclusion, we comprehensively illustrated the mechanism of XHJ natural autumn shooting through cytological, metabolic, and transcriptomic analysis, which improves our understanding of the high yield of XHJ rhizomes and the diversity of shooting mechanisms in Polygonatum to lay the foundation for the further development of the Huangjing industry.
Social support, network, and relationships among coaches in different sports: a systematic review
Authors: Ferreira João Gonçalo , Rodrigues Filipe , Sobreiro Pedro , Silva Mário , Santos Fernando Jorge , Carvalho Gonçalo , Hernández Mendo António , Rodrigues José
Published: September 2024

Introduction

The study aims to analyze scientific publications on the association between social networks, social relationships, and social support for sports coaches. It seeks to identify the types and levels of social support provided by various agents, and to understand the impact of this support on coaches’ wellbeing. The goal is to help coaches better utilize social support, thereby enhancing their quality of life, work, and performance.

Methods

This study systematically reviewed 11 scientific articles to investigate the association between social support, social networks, and social relationships in sports coaches. It aimed to identify the types and levels of social support offered to coaches by family members, peers, and friends. Our research utilized the PRISMA guidelines for systematic reviews and assessed study quality using the STROBE Statement. Eligibility was determined by the PECOS criterion based on the search strategy terms.

Results

Our findings indicate that social support has significant positive effects on sports coaches. It enhances selfcompassion, prevents burnout symptoms, boosts job and life satisfaction, and reduces stress levels. Organizational support, characterized by clear guidelines, guidance, and autonomy, yielded positive outcomes. Conversely, the absence of social support correlated with negative outcomes for coaches, including lower self-compassion, increased stress and burnout symptoms, reduced job and life satisfaction, and heightened work–family conflict. Coaches’ social networks encompassed family members, peers, friends, and other sources, with friends perceived as the most influential. Maintaining an effective social support network is crucial for coaches’ performance and psychological wellbeing.

Discussion

This systematic review emphasizes the importance of social support for coaches in both their personal and professional lives, noting its positive effects and the negative consequences of its absence. Given the demanding nature of coaching, improving social support systems can enhance coaches’ wellbeing and the success of sports activities.
Testing the validity of the broaden-and build theory of positive emotions: a network analytic approach
Authors: Roth Leopold Helmut Otto , Bencker Celine , Lorenz Johanna , Laireiter Anton-Rupert
Published: September 2024

Introduction

The Broaden-and-Build Theory of positive emotions is one of the best known and applied theories in Positive Psychology. It argues that positive emotions initiate an upward movement by opening up the mind and broadening thoughts and thus represents a counter model to the vicious circle-models of clinical psychology. The number of studies directly testing this theory in all components is scarce, ambiguities in the model impede clear inference.

Method

To draw a conclusive picture on within-model processes, we applied network modeling on the components of the theory across two studies (N1 = 312; N2 = 302).

Results

In both studies, the positive relationship between positive emotions, resources and life outcomes is well-supported, yet the role of broadening, as an intermediary component within these is questioned.

Discussion

As the broadening component consistently deviated from the model’s predictions and thus did not contribute to the model as expected in either study, the validity of the Broaden-and-Build Theory in its current conceptualization is challenged, and our results point to the need to reassess the role of broadening.