bluuebunny/crossref_metadata_2025 huggingface dataset:
Bilateral neck fracture in bimodular femoral stem after primary total hip arthroplasty: a case report
Authors: Fokter Samo K. , Gubeljak Nenad , Predan Jožef , Sevšek Jure , Zajc Jan , Krajnc Zmago
Published: April 2021

Background

Bi-modular stems were introduced in primary total hip arthroplasty (THA) to enable better control of the femoral offset, leg length, and hip stability. Despite numerous reports on modular femoral neck fractures, some designs are still marketed worldwide. While the risk factors for the sudden failure are multifactorial and mostly known, the timing of this new THA complication is not predictable by any means.

Case presentation

In this report, the literature regarding one of the most popular bi-modular stems with specific neck-stem coupling (oval Morse taper) is reviewed and illustrated with a case of bilateral modular neck fracture in a patient with idiopathic aseptic necrosis of femoral heads treated with primary bi-modular THA. Because of bilateral modular femoral neck fracture, which occurred 3 years on the left side and 20 years after implantation on the right side, the patient required a total of 6 revisions and 208 days of hospitalized care.

Conclusion

To our knowledge, this is the first report of bilateral modular neck fracture in a single patient. Even though the same surgeon performed both operations and used the same neck length and orientation, fractures occurred with a 17-year time difference after implantation. This shows that we cannot predict with certainty when a fracture might occur. Orthopaedic surgeons should use bi-modular stem designs for primary THA very cautiously.
Comparison between two cell collecting methods for liquid-based brush biopsies: a consecutive and retrospective study
Authors: Gaida Kristin , Deuerling Lena , Neumann Heinrich , Remmerbach Torsten W.
Published: April 2021

Background

This study compares two different cell collectors, the Orcellex Brush (rigid brush) and the Cytobrush GT (nylon brush), using liquid-based cytology. A comparison of their obtainment procedures was also considered. The aim was to determine the diagnostic accuracy for detection of malignancy in oral brush biopsies. PICO-Statement: In this consecutive and retrospective study we had as population of interests, patients with oral lesions, the intervention was the brush biopsy with two different cell collectors and the control was healthy oral mucosa. The outcome of the study was to compare both cell collectors.

Methods

From 2009 to 2018, 2018 patients with oral lesions were studied using the nylon brush (666 cases) and rigid brush (1352 cases). In the first cohort five smears per patient were taken with the nylon brush, while each patient received one smear with the rigid brush in the second cohort. These were further processed in a liquid-based procedure. Cytological evaluations were categorised into ‘negative’, which were considered as negative, whereas ‘doubtful’, ‘suspicious’ and ‘positive’ cytological results were overall considered as positive for malignancy in comparison to the final histological diagnoses. Additionally, the clinical expenditure for each collector was estimated.

Results

2018 clinically and histologically proven diagnoses were established, including 181 cases of squamous cell carcinomas, 524 lichen, 454 leukoplakias, 34 erythroplakias and 825 other benign lesions. The sensitivity and specificity of the nylon brush was 93.8% (95% CI 91.6–95.5%) and 94.2% (95% CI 91.8–95.5%) respectively, whereas it was 95.6% (95% CI 94.4–96.6%) and 84.9% (95% CI 83.8–87.5%) for the rigid brush. The temporal advantage using the plastic brushes was 4×  higher in comparison to the nylon brush. The risk suffering from a malignant oral lesion when the result of the brushes was positive, suspicious, or doubtful was significantly high for both tests (nylon brush OR: 246.3; rigid brush OR: 121.5).

Conclusions

Both systems have a similar sensitivity, although only the rigid brush achieved a satisfactory specificity. Additional methods, such as DNA image cytometry, should also be considered to improve the specificity. Furthermore, the rigid brush proved to be more effective at taking a sufficient number of cells, whilst also being quicker and presenting less stress for the patient.
An Integrated eDiagnosis Approach (IeDA) versus standard IMCI for assessing and managing childhood illness in Burkina Faso: a stepped-wedge cluster randomised trial
Authors: Sarrassat Sophie , Lewis James J. , Some Arsene S. , Somda Serge , Cousens Simon , Blanchet Karl
Published: April 2021

Background

The Integrated eDiagnosis Approach (IeDA), centred on an electronic Clinical Decision Support System (eCDSS) developed in line with national Integrated Management of Childhood Illness (IMCI) guidelines, was implemented in primary health facilities of two regions of Burkina Faso. An evaluation was performed using a stepped-wedge cluster randomised design with the aim of determining whether the IeDA intervention increased Health Care Workers’ (HCW) adherence to the IMCI guidelines.

Methods

Ten randomly selected facilities per district were visited at each step by two trained nurses: One observed under-five consultations and the second conducted a repeat consultation. The primary outcomes were: overall adherence to clinical assessment tasks; overall correct classification ignoring the severity of the classifications; and overall correct prescription according to HCWs’ classifications. Statistical comparisons between trial arms were performed on cluster/step-level summaries.

Results

On average, 54 and 79% of clinical assessment tasks were observed to be completed by HCWs in the control and intervention districts respectively (cluster-level mean difference = 29.9%; P-value = 0.002). The proportion of children for whom the validation nurses and the HCWs recorded the same classifications (ignoring the severity) was 73 and 79% in the control and intervention districts respectively (cluster-level mean difference = 10.1%; P-value = 0.004). The proportion of children who received correct prescriptions in accordance with HCWs’ classifications were similar across arms, 78% in the control arm and 77% in the intervention arm (cluster-level mean difference = − 1.1%; P-value = 0.788).

Conclusion

The IeDA intervention improved substantially HCWs’ adherence to IMCI’s clinical assessment tasks, leading to some overall increase in correct classifications but to no overall improvement in correct prescriptions. The largest improvements tended to be observed for less common conditions. For more common conditions, HCWs in the control districts performed relatively well, thus limiting the scope to detect an overall impact.

Trial registration

ClinicalTrials.gov NCT02341469; First submitted August 272,014, posted January 19, 2015.
Analysis of single nucleotide polymorphisms in chronic beryllium disease
Authors: Frye Björn C. , Gaede Karoline I. , Saltini Cesare , Rossman Milton D. , Monos Dimitri S. , Rosenman Ken D. , Schuler Christine R. , Weston Ainsley , Wegner Ralf , Noth Rainer , Zissel Gernot , Schreiber Stefan , Nothnagel Michael , Müller-Quernheim Joachim
Published: April 2021

Sarcoidosis and chronic beryllium disease (CBD) are phenocopies, however the latter one has a clear trigger factor that is beryllium exposure. This study analyses single nucleotide polymorphisms (SNPs) in a large cohort for beryllium-exposed persons. SNPs were chosen for their relevance in sarcoidosis. Even though one of largest cohorts of beryllium-exposed persons was analysed, no statistically relevant association between any SNP and CBD could be verified. Notably, some SNPs exhibit inverse OR for beryllium sensitization and CBD with nominally statistical significance, which allows hypothesizing about pathophysiological role of genes for the disease triggering and development.
Nanomaterials and hepatic disease: toxicokinetics, disease types, intrinsic mechanisms, liver susceptibility, and influencing factors
Authors: Sun Ting , Kang Yiyuan , Liu Jia , Zhang Yanli , Ou Lingling , Liu Xiangning , Lai Renfa , Shao Longquan
Published: April 2021

The widespread use of nanomaterials (NMs) has raised concerns that exposure to them may introduce potential risks to the human body and environment. The liver is the main target organ for NMs. Hepatotoxic effects caused by NMs have been observed in recent studies but have not been linked to liver disease, and the intrinsic mechanisms are poorly elucidated. Additionally, NMs exhibit varied toxicokinetics and induce enhanced toxic effects in susceptible livers; however, thus far, this issue has not been thoroughly reviewed. This review provides an overview of the toxicokinetics of NMs. We highlight the possibility that NMs induce hepatic diseases, including nonalcoholic steatohepatitis (NASH), fibrosis, liver cancer, and metabolic disorders, and explore the underlying intrinsic mechanisms. Additionally, NM toxicokinetics and the potential induced risks in the livers of susceptible individuals, including subjects with liver disease, obese individuals, aging individuals and individuals of both sexes, are summarized. To understand how NM type affect their toxicity, the influences of the physicochemical and morphological (PCM) properties of NMs on their toxicokinetics and toxicity are also explored. This review provides guidance for further toxicological studies on NMs and will be important for the further development of NMs for applications in various fields.
Addressing gender imbalance in intensive care
Authors: Vincent Jean-Louis , Juffermans Nicole P. , Burns Karen E. A. , Ranieri V. Marco , Pourzitaki Chryssa , Rubulotta Francesca
Published: April 2021

There is a large gender gap in critical care medicine with women underrepresented, particularly in positions of leadership. Yet gender diversity better reflects the current critical care community and has multiple beneficial effects at individual and societal levels. In this Viewpoint, we discuss some of the reasons for the persistent gender imbalance in critical care medicine, and suggest some possible strategies to help achieve greater equity and inclusion. An explicit and consistent focus on eliminating gender inequity is needed until gender diversity and inclusion become the norms in critical care medicine.
The association between cardiorespiratory fitness, liver fat and insulin resistance in adults with or without type 2 diabetes: a cross-sectional analysis
Authors: Sabag Angelo , Keating Shelley E. , Way Kimberley L. , Sultana Rachelle N. , Lanting Sean M. , Twigg Stephen M. , Johnson Nathan A.
Published: April 2021

Background

Exercise-induced improvements in cardiorespiratory fitness (CRF) often coincide with improvements in insulin sensitivity and reductions in liver fat content. However, there are limited data concerning the relationship between CRF and liver fat content in adults with varying degrees of metabolic dysfunction.

Methods

The aim of this study was to examine the association between CRF, liver fat content, and insulin resistance in inactive adults with obesity and with or without type 2 diabetes (T2D), via cross-sectional analysis. CRF was determined via a graded exercise test. Liver fat content was assessed via proton magnetic resonance spectroscopy and insulin resistance was assessed via homeostatic model of insulin resistance (HOMA-IR). A partial correlation analysis, controlling for age and gender, was performed to determine the association between CRF, demographic, cardiometabolic, and anthropometric variables. Independent t tests were performed to compare cardiometabolic outcomes between participants with T2D and participants without T2D.

Results

Seventy-two adults (46% male) with a mean age of 49.28 ± 10.8 years, BMI of 34.69 ± 4.87 kg/m2, liver fat content of 8.37 ± 6.90%, HOMA-IR of 3.07 ± 2.33 and CRF of 21.52 ± 3.77 mL/kg/min participated in this study. CRF was inversely associated with liver fat content (r = − 0.28, p = 0.019) and HOMA-IR (r = − 0.40, p < 0.001). Participants with T2D had significantly higher liver fat content (+ 3.66%, p = 0.024) and HOMA-IR (+ 2.44, p < 0.001) than participants without T2D. Participants with T2D tended to have lower CRF than participants without T2D (− 1.5 ml/kg/min, p = 0.094).

Conclusion

CRF was inversely associated with liver fat content and insulin resistance. Participants with T2D had lower CRF than those without T2D, however, the difference was not statistically significant. Further longitudinal studies are required to elucidate the relationship between CRF and the progression of obesity-related diseases such as T2D.

Registration: ACTRN12614001220651 (retrospectively registered on the 19th November 2014) and ACTRN12614000723684 (prospectively registered on the 8th July 2014).
Cold agglutinin-induced hemolytic anemia during room temperature fluid resuscitation: a case report
Authors: Kawai Yosuke , Deguchi Miyoshi , Mizouchi Naoko , Yoshida Satoru , Kumagai Ken , Hirose Yasuo
Published: April 2021

Background

Cold agglutinin disease can cause the agglutination of red blood cells and hemolytic anemia due to cold temperature. Herein, we report a case of progressive hemolytic anemia due to cold agglutinin disease during fluid resuscitation and in the absence of exposure to cold.

Case presentation

A 71-year-old Japanese man was admitted to the emergency department with signs of hypotension and disturbed consciousness. He was diagnosed with diabetic ketoacidosis, and treatment with fluid resuscitation and insulin infusion was initiated. Laboratory test results obtained the following day indicated hemolytic anemia. On day 5 after admission, red blood cell agglutination was detected, and the patient was diagnosed with cold agglutinin disease.

Conclusions

Cold agglutinin disease should be considered in the differential diagnosis of progressive hemolytic anemia during fluid resuscitation, even if the solution is at room temperature.
Clozapine-induced stuttering in the absence of known risk factors: a case report
Authors: Jaguga Florence
Published: April 2021

Background

Stuttering is a rare side effect of clozapine. It has been shown to occur in the presence of one or more factors such as abnormal electrophysiological findings and seizures, extrapyramidal symptoms, brain pathology, and a family history of stuttering. Few case reports have documented the occurrence of clozapine-induced stuttering in the absence of these risk factors.

Case presentation

A 29-year-old African male on clozapine for treatment-resistant schizophrenia presented with stuttering at a dosage of 400 mg/day that resolved with dose reduction. Electroencephalogram findings were normal, and there was no clinical evidence of seizures. The patient had no prior history or family history of stuttering, had a normal neurological examination, and showed no signs of extrapyramidal symptoms.

Conclusion

Clinicians ought to be aware of stuttering as a side effect of clozapine, even in the absence of known risk factors. Further research should investigate the pathophysiology of clozapine-induced stuttering.
Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis
Authors: Gondaira Satoshi , Nishi Koji , Fujiki Jumpei , Iwano Hidetomo , Watanabe Reina , Eguchi Ayako , Hirano Yuki , Higuchi Hidetoshi , Nagahata Hajime
Published: April 2021

Mycoplasma bovis(M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection withM. bovis. However, even though neutrophils accumulate inM. bovisinfection, the interaction ofM. bovisand neutrophils has not been fully elucidated. We attempted to elucidate the innate immune response of neutrophils stimulated withM. bovisand evaluate the transcriptome and functional analysis of bovine neutrophils stimulated withM. bovis. Proinflammatory cytokines, such as inducible nitric oxide (iNOS), which was the most increased gene in transcriptome analysis, were increased in quantitative polymerase chain reaction analysis of bovine neutrophils stimulated with live or heat-killedM. bovis. Nitric oxide and intracellular reactive oxygen species production of neutrophils stimulated withM. boviswas significantly increased. Neutrophils stimulated withM. bovisshowed an increased ratio of nonapoptotic cell death compared to unstimulated controls. We demonstrated that neutrophil extracellular traps (NETs) formation was not recognized in neutrophils stimulated with liveM. bovis. However, heat-killedM. bovisinduced NETs formation. We also showed the interaction withM. bovisand bovine neutrophils regarding proinflammatory cytokine gene expression and functional expression related to NETs formation. Live and killedM. bovisinduced innate immune responses in neutrophils and had the potential to induce NETs formation, but liveM. bovisescaped NETs.
Opportunities for successful de-escalation of proton pump inhibitors at a federally qualified health center
Authors: Ayoub Joelle , McGregor Jessina C. , Castner Rebecca M. , Singh Harleen
Published: April 2021

Background

A Proton Pump Inhibitor (PPI) de-escalation initiative was piloted at a Family Medicine Federally Qualified Health Center (FQHC) after a needs assessment showed that PPIs were prescribed inappropriately. The objective was to evaluate implementation of a PPI de-escalation program for an urban, underinsured patient population at a (FQHC).

Methods

Patients receiving PPI with an upcoming appointment with their primary care provider (PCP) were evaluated by a pharmacist for the appropriateness of therapy. The pharmacist administered a questionnaire to patients to assess PPI usage patterns and then evaluated for appropriate PPI therapy which included diagnoses, risk factors for gastrointestinal bleed, symptom control, and duration of PPI therapy. For consenting patients, de-escalation was implemented per pharmacist protocol.

Results

A total of 36 patients were evaluated for appropriate PPI use, among those, 21 (58%) were eligible for de-escalation, and 19 agreed to de-escalation. Fifteen patients (15/19) had successful PPI de-escalation after 4 weeks without discomfort or symptoms which disrupted daily activities.

Conclusions

This pharmacist led initiative in collaboration with PCPs resulted in successful de-escalation of PPIs in an underserved primary care setting.
On a simple, data-aided analytic description of the morphology of equatorial F-region zonal plasma drifts
Authors: Shidler Samuel A. , Rodrigues Fabiano S.
Published: April 2021

We present results of an effort to evaluate the ability of an analytic model to describe the behavior of the equatorial zonal plasma drifts given inputs provided by readily available climatological models of thermospheric and ionospheric parameters. In a data-model fusion approach, we used vertical drift measurements to drive the model and zonal drift measurements to evaluate its output. Drift measurements were made by the Jicamarca incoherent scatter radar, and model results were evaluated for different seasons and two distinct solar flux conditions. We focused, in particular, on model results for different versions of the Horizontal Wind Model (HWM 97, 07, and 14). We found that, despite its simplicity, the analytic model can reproduce fairly well most of the features in the observed zonal plasma drifts, including the vertical shear associated with the evening plasma vortex. During daytime hours the model predicts similar results for the zonal drifts independently of the HWM used to drive the model. More importantly, the modeled daytime drifts match exceptionally well the behavior and magnitude of the observed drifts for all seasons and solar flux conditions considered. The nighttime results drive the overall performance of the analytic model, and we found that a single HWM cannot provide the best results for all seasons and solar flux conditions. We also examined the main sources of zonal drift variability. Most of the morphology is controlled by the zonal wind dynamo term of the analytic model, but with non-negligible contribution from the vertical drift term. Finally, we examined the contribution from the E- and F-region to the zonal wind dynamo. The morphology of the zonal drifts in the region of observation (240–560 km altitude) is controlled mostly by the F-region winds, but with significant contributions from the daytime E-region particularly during December solstice and low solar flux conditions.
Clinical performance of narrow-diameter titanium–zirconium implants in immediately loaded fixed full-arch prostheses: a 2-year clinical study
Authors: Coskunses Fatih Mehmet , Tak Önjen
Published: April 2021

Objectives

The aim of this study was to evaluate the outcomes of immediate fixed full-arch prostheses supported by axial or tilted narrow-diameter Ti-Zr implants (3.3 mm) (Roxolid®, Institut Straumann® AG, Basel, Switzerland) (NDIs) in combination with standard-diameter implants up to 2 years’ follow-up.

Materials and methods

The study was conducted at Kocaeli University Faculty of Dentistry from 2016 to 2018. 37 jaws of 28 patients with an average age of 52 years were rehabilitated with fixed full-arch prostheses supported by 179 implants. Cumulative survival rate (CSR), implant success, marginal bone loss (MBL), and prosthetic survival rate as well as complications were analyzed.

Results

Total CSR of 99.4% and 98.5% for all and narrow implants respectively have been observed at 2 years’ follow-up. No prosthesis failures were observed, yielding a cumulative prosthetic survival rate of 100%. The NDIs achieved 0.63 mm MBL at 1 year and 1.02 mm at 2 years. The mean MBL at 1 year was 0.51 mm (mandible 0.63 mm/maxilla 0.41 mm) and 0.73 mm (mandible 0.90 mm/maxilla 0.43 mm) at 2 years. Both implant angulation and loading protocol did not influence the MBL.

Conclusions

The combination of narrow-diameter implants with standard-diameter implants in immediate fixed full-arch rehabilitation has a good prognosis to become a new standard of care for severely atrophic jaws.

Clinical relevance

The use of narrow-diameter implants in fixed full-arch rehabilitations in atrophic ridges would be a successful and predictable treatment approach.
Correction to: Establishment and effectiveness evaluation of a scoring system for exertional heat stroke by retrospective analysis
Authors: Yang Meng-Meng , Wang Lu , Zhang Yu , Yuan Rui , Zhao Yan , Hu Jie , Zhou Fei-Hu , Kang Hong-Jun
Published: April 2021

An amendment to this paper has been published and can be accessed via the original article.
A custom virtual reality training solution for ophthalmologic surgical clinical trials
Authors: Heimann Felix , Barteselli Giulio , Brand André , Dingeldey Andreas , Godard Laszlo , Hochstetter Hendrik , Schneider Michael , Rothkegel Alexander , Wagner Clemens , Horvath Joshua , Ranade Shrirang
Published: April 2021

We present a summary of the development and clinical use of two custom designed high-fidelity virtual-reality simulator training platforms. This simulator development program began in 2016 to support the phase III clinical trial Archway (ClinicalTrials.gov identifier, NCT03677934) intended to evaluate the Port Delivery System (PDS) developed by Genentech Inc. and has also been used to support additional clinical trials. The two simulators address two specific ophthalmic surgical procedures required for the successful use of PDS and provide state-of-the-art physical simulation models and graphics. The simulators incorporate customized active haptic feedback input devices that approximate different hand pieces including a custom hand piece specifically designed for PDS implantation. We further describe the specific challenges of the procedure and the development of corresponding training strategies realized within the simulation platform.
Comprehensive power swing detection by current signal modeling and prediction using the GMDH method
Authors: Hosseini Seyed Amir , Taheri Behrooz , Abyaneh Hossein Askarian , Razavi Farzad
Published: April 2021

Power swing is an undesirable variation in power flow. This can be caused by large disturbances in demand load, switching, disconnection or reclosing lines. This phenomenon may enter the zones of distance relays and cause relay malfunction leading to the disconnection of healthy lines and undermining network reliability. Accordingly, this paper presents a new power swing detection method based on the prediction of current signal with a GMDH (Group Method of Data Handling) artificial neural network. The main advantage of the proposed method over its counterparts is the immunity to noise effect in signals. In addition, the proposed method can detect stable, unstable, and multi-mode power swings and is capable of distinguishing them from the variety of permanent faults occurring simultaneously. The method is tested for different types of power swings and simultaneous faults using DIgSILENT and MATLAB, and compared with some latest power swing detection methods. The results demonstrate the superiority of the proposed method in terms of response time, the ability to detect power swings of different varieties, and the ability to detect different faults that may occur simultaneously with power swings.
Pathogenicity induced by indigenous Beauveria bassiana isolate in different life stages of the cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) under laboratory conditions
Authors: Fergani Yasmin Adel , Refaei Elsayed Abd Elhameed
Published: April 2021

Background

The invincible effects of the entomopathogenic fungi (EPF) under appropriate circumstances compensate for the flaws of chemical insecticides in the control programs. Beauveria bassiana is one of the most elaborated EPF of pest control all over the world. The potential of using the B. bassiana isolate (Y-F_ITS1) was examined against different larval instars (L2, L3, L4, and L5), pupae, and eggs of the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), using different concentrations under laboratory conditions.

Results

In bioassay, 2nd instar larvae of S. littoralis were significantly the most susceptible stage to B. bassiana isolate at P ≤ 0.05. It is apparent from the results that the cumulative mortality percentage increased with elapsing time with typical symptoms of infection and sporulation. High efficacies ranging from 85.0 to 99.0% corrected mortality rates were recorded for 2nd instar larvae at the spore concentration (1 × 109 spores ml−1). The mortality percentage reached 90.0% for the 3rd instar larvae treated with the same concentration after 5 days. The least mortality rates results were recorded at the 5th instar larvae. B. bassiana isolate showed an ovicidal effect to the eggs of S. littoralis that reached 100% mortality when treated with 1.0 × 108 and 1.0 × 109 spores ml−1, while the concentration 1 × 107 and 1.0 × 108 caused 65.0 and 87.0%, respectively. Reduction of adult emergence reached (0%) in case of treatment with the highest concentrations (1 × 108 and 1 × 109 spores ml−1). Morphogenetic abnormalities were also recorded.

Conclusion

Results of mortality rates and lethal concentration values resulted from the experiments indicated that the tested Y-F_ITS1 isolate was efficient and can be recommended as a potential biocontrol agent against S. littoralis. Further field evaluations are still needed.
Hypertrophic cardiomyopathy with paroxysmal atrial fibrillation misdiagnosed as WPW syndrome
Authors: Seol Sang-Hoon , Kim Ki-Hun , Park Jino , Song Yeo-Jeong , Kim Dong-Kie , Kim Doo-Il
Published: April 2021

Hypertrophic cardiomyopathy (HCM) is associated with an increased incidence of Wolff–Parkinson–White (WPW) syndrome and atrial fibrillation. However, a delta-like wide QRS can be observed in the hypertrophied myocardium. When considering the rarity of the paraseptal bypass tract (BT), the normal QRS axis suggests a higher possibility of HCM origin. Otherwise, there is no known electrocardiographic clue indicating a wide QRS differentiation between HCM and WPW syndrome. Moreover, the atriofascicular, nodofascicular/ventricular or fasciculoventricular BT should be differentiated. In this case, atrioventricular conduction system incidental injury revealed a wide QRS origin from the HCM, but this method should be avoided except in some selected cases.
Surgical planning in patients with brain glioma using diffusion tensor MR imaging and tractography
Authors: Shalan Mohamed E. , Soliman Ahmed Y. , Nassar Ibrahim A. , Alarabawy Reda A.
Published: April 2021

Background

Diffusion-tensor imaging (DTI) is a magnetic resonance imaging (MRI) technique that depicts the integrity of white matter (WM) tracts. This study was conducted to assess the utility of DTI tractography as an imaging technique in assessment of brain gliomas and planning of the surgical corridor.

Results

Twenty adult patients with brain gliomas were included. Neurological examination and conventional MRI and DTI scans were performed before and after surgery. Low-grade and high-grade tumors were found in 30% and 70% of patients, respectively. Preoperative DTI demonstrated five patterns of WM tract involvement: non-affected (10%), displaced (75%), edematous (55%), infiltrated (60%), and disrupted (20%). The obtained DTI scans were used for preoperative planning of the surgical corridor and extent of resection to achieve the maximum resection while preserving the WM tracts. Total resection was achieved in 40%, while 60% underwent subtotal resection. Postoperative neurological examination showed deterioration of cognitive function, motor power, and vision in 15%, 10%, and 5% of patients, respectively. Headache persisted in 15%, while motor power improved in 35% of patients. High-grade tumors were significantly associated with higher percentage of subtotal resection (p=0.018) and pattern IV (p=0.018). There was a significant association between the preoperative pattern of WM tract involvement and the postoperative DTI changes (p<0.001).

Conclusion

DTI enables assessment of displaced and infiltrated WM tracts in the vicinity of brain tumors. Preoperative planning of tumor resection and surgical corridor should include DTI scan to achieve the balance between maximum resection of tumor and maximal preservation of function.
Analysis methodology and assessment indices of vulnerability for asphalt pavement in cold regions
Authors: Wang Tongxu , Ma Xianyong , Li Huanyu , Dong Zejiao
Published: April 2021

Asphalt pavement structures in cold regions, which suffer from complicated environmental and geological conditions, such as large temperature difference and frozen soil, are prone to cracking, rutting, and moisture damage. However, most of the existing assessment methodologies focus on the vulnerability of the overall road traffic network, ignoring the impact of regional differences and pavements’ structural performance. To establish a highly targeted vulnerability analysis methodology for cold regional asphalt pavements, the concept of highway vulnerability and the assessment model composed of exposure, fragility, and resilience were proposed in this paper firstly. Meanwhile, the assessment indices and standards for exposure, fragility, and resilience were respectively discussed. Then, the calculation process for each index weight and vulnerability index was proposed based on AHP-fuzzy comprehensive assessment methodology. Consequently, the vulnerability grade of asphalt pavements in cold regions could be determined. Finally, the vulnerability assessment indices and methodology for cold regional asphalt pavements were illustrated and presented, providing a theoretical basis for asphalt pavement performance evaluation and vulnerability assessment serviced under cold regional climate.
Reverse phase-liquid chromatography assisted protocol for simultaneous determination of lamivudine and tenofovir disoproxil fumarate in combined medication used to control HIV infection: an investigative approach
Authors: Adhao Vaibhav S. , Chaudhari Suraj R. , Ambhore Jaya P. , Sangolkar Sunil , Thenge Raju R. , Cheke Rameshwar S. , Patil Amod S.
Published: April 2021

Background

Human immunodeficiency virus (HIV) causes severe life-threatening condition, i.e., AIDS. HIV destabilises an individual’s ability to prevent infection. Therefore, the combine medication lamivudine (LVD) and tenofovir disoproxil fumarate (TDF) are prescribed to suppress the amount of HIV infection in individual’s body; thus, the individual’s immune system could function properly. Consequently, the objective of present research work was to investigate robust and sensitive liquid chromatography avenue for simultaneous determination of lamivudine and tenofovir disoproxil fumarate in pure material and combined dosage form.

Results

The reversed-phase chromatographic separation has been performed through Hypersil BDS C18 column using solvent system composed of 10 mM potassium dihydrogen phosphate (pH 4.0): acetonitrile (60:40% v/v). The determination was executed at 30 oC at 1 mL/min rate for flow of solvent system through column. The eluents of column were monitored at 265 nm using Photodiode Array detector has revealed admirable retention times, i.e., 4.67 and 8.78 min for both drugs, respectively. The calibration curve demonstrated excellent linearity in the range of 10–50 μg/mL for lamivudine and tenofovir disoproxil fumarate with better determination coefficients was more than (r2 0.999).

Conclusion

The estimable method was effectively validated with respect to accuracy, precision, sensitive (limit of detection and limit of quantitation), robustness, ruggedness, and for selectivity and specificity. The value less than 2 for percentage relative standard deviation for accuracy, precision, robustness, and ruggedness satisfying the acceptance criteria as per procedure of International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use.
Behavioral changes in the brown planthopper, Nilaparvata lugens, mediated by melatonin
Authors: Shi Longqing , Zhang Junian , Qiu Liangmiao , Jiang Zhaowei , Xie Zhenxing , Zhan Zhixiong
Published: April 2021

Background

Melatonin has been proved to exist and play importance roles in rice plant, such as biosynthesis and resistance. However, little is known about the function of melatonin in its monophagous pest, the brown planthopper.

Methods

In this study, we examined the effects of melatonin on the copulatory and locomotor behaviors of brachypterous and macropterous adult planthoppers by exposing them to melatonin, luzindole (a melatonin receptor antagonist), or a combination of melatonin and luzindole.

Results

A total of 68.7% of copulation events occurred at night in the control, while 31.2% occurred at night in the melatonin treatment, which led to a decrease in offspring. Brachypterous males were involved in mating events in the melatonin treatment but not in the other two treatments or the control. The daily locomotor pattern in the melatonin treatment was markedly different from that in the luzindole and melatonin and luzindole treatments. The total locomotor activities of the macropterous and brachypterous males exposed with melatonin were suppressed compared to those in the control. Melatonin significantly decreased the daytime and nighttime locomotor activities of macropterous females. In comparison, the activity of brachypterous females decreased slightly in the daytime but was more than double that of the control females at night.

Conclusions

Our results reveal that melatonin plays a role in the behaviors of brown planthoppers.
Sedimentary system and sand bodies distribution of the second member of the Xujiahe Formation in the Xinchang area, Western Sichuan Depression, China
Authors: Shang Xiaofei , Li Meng , Duan Taizhong
Published: April 2021

The Xujiahe Formation of the Late Triassic in the Western Sichuan Depression contains abundant gas reservoirs. Influenced by the thrust tectonic movement of the foreland basin, the fluvial-delta sedimentary system supplied by multiple provenances formed the Xu2 Formation of the Xinchang area. We used detailed description of drilling wells and cores to define the sequence stratigraphic framework and sand body types. We used stratal slices through the seismic texture model regression (TMR) attribute volume to map the evolution of the sedimentary system and the sand body distribution. Our results indicate that the Xu2 Formation exhibits a complete long-term base-level cycle and that there are six sand body deposit types: distributary channel, interchannel, subaqueous distributary channel, interdistributary bay, mouth bar, and sheet sand. Stratal slices through the seismic TMR attribute volume at different levels map the spatial variation of sand and mudstone, which we use to construct a sedimentary filling evolution model. This model indicates that during the time of deposition of the lower submember, the main provenance supply came from the northwest direction, resulting in the sand bodies mainly being deposited in the west. During the time of deposition of the central submember, the sediment supply was large and came from the northwest and northeast directions, resulting in large, laterally extensive, thick sands. During the time of deposition of the upper submember, the sediment supply was from the northeast direction, with the sand bodies more developed in the east. The flow direction of the (subaqueous-) distributary channels indicate that they migrated from northwest to northeast. There are significant differences in the channel energy, sedimentary characteristics, and reservoir physical properties in the three submembers, which cause differences in oil and gas productivity in the reservoir of the Xu2 Formation. We believe that detailed mapping of the spatial distribution of sedimentary systems can provide critical guidance not only to explore but also to develop in high-quality oil and gas reservoirs such as the Xu2 Formation.
TSH Activates Macrophage Inflammation by G13- and G15-dependent Pathways
Authors: Yang Chongbo , He Zhao , Zhang Qunye , Lu Ming , Zhao Jiajun , Chen Wenbin , Gao Ling
Published: April 2021

Thyroid-stimulating hormone (TSH) treatment activates inhibitor of NF-κB/nuclear factor κB (IκB/NFκB) and extracellular signal-regulated kinase (ERK)–P38 in macrophages, but how these pathways are activated, and how they contribute to the proinflammatory effect of TSH on macrophages remain unknown. The TSH receptor (TSHR) is coupled to 4 subfamilies of G proteins (Gs, Gi/o, Gq/11, and G12/13) for its downstream signaling. This study investigated the G protein subtypes responsible for the proinflammatory effect of TSH on macrophages. qPCR showed that Gi2, Gi3, Gas, Gq, G11, G12, G13, and G15 are abundantly expressed by macrophages. The contribution of different G protein pathways to the proinflammatory effect was studied by the corresponding inhibitors or siRNA interference. While TSH-induced IκB phosphorylation was not inhibited by Gs inhibitor NF449, Gi inhibitor pertussis toxin, or Gq or G11 siRNA, it was blocked by phospholipase C inhibitor U73122 or G15 siRNA interference. TSH-induced ERK and P38 phosphorylation was blocked by G13 but not G12 siRNA interference. Interference of either G13 or G15 could block the proinflammatory effect of TSH on macrophages. The present study demonstrate that TSH activates macrophage inflammation by the G13/ERK–P38/Rho GTPase and G15/phospholipase C (PLC)/protein kinases C (PKCs)/IκB pathways.
Giant Prolactinoma Presenting With Facial Nerve Palsy and Hemiparesis
Authors: Sliwinska Aleksandra , Jalil Fatima , De La Portilla Lori , Baldwin Michael , Lorenzo Joseph , Bulsara Ketan R , Mirza Faryal S
Published: April 2021

Background

Giant prolactinomas are an exceedingly uncommon type of pituitary adenomas that usually occur in men, and cause extremely high prolactin levels and mass-related symptoms. Rarely, patients may experience neurological deficits resembling ischemic events.

Methods

We describe an unusual case of a young man who presented with stroke-like symptoms and was found to have a giant prolactinoma.

Clinical Case

A 25-year-old man presented with left facial droop and gradually progressing upper and lower extremity weakness for evaluation of stroke. He reported recent weight gain and erectile dysfunction. Physical examination revealed left homonymous hemianopsia, left VII nerve palsy, and left hemiparesis. Magnetic resonance imaging of the brain showed an enormous mass in the sella turcica, which invaded the sphenoid sinus and right side of the skull base. Prolactin level was elevated at 13 580 ng/mL, and the testosterone level was low. The patient was started on cabergoline and had marked improvement in his symptoms in a few months. Fifteen months after starting treatment, he has had more than 90% reduction in tumor volume and a 93% reduction in prolactin level.

Conclusion

Giant prolactinomas are uncommon and present with compressive symptoms that can be mistaken for a stroke. Our case is a unique report of a facial nerve palsy and hemiparesis secondary to giant prolactinoma in the absence of stroke or pituitary apoplexy.
The Distribution of Radioactive Potassium in the Uterus of Pregnant Rats and Guinea-pigs
Authors: D’silva J. L. , Harrison R. J.
Published: April 2021

The distribution of tracer amounts of 42KC1 was studied in the kidney, placenta, uterus, and foetus (a) 5 minutes to 24 hours after injection into pregnant rats (20-21 days), and (b) 5 minutes after injection into pregnant rats (11-18 days) and guinea-pigs (near term). “ The changes in activity in the kidney and foetus (per gramme of tissue) indicate that the former exchanges its potassium rapidly and the latter slowly. The activity of the labyrinthine placenta of rats near term falls from 0·53 per cent, (per gramme of tissue) of the total injected radioactivity after 5 minutes to 0·29 per cent, after 24 hours. The activity of the uterus changes similarly and suggests a rapid rate of exchange and a slow blood-flow. The radioactivity of the uterus was not significantly different in the nonpregnant uterus, the non-pregnant horn of the pregnant uterus, and the pregnant horn. The placentae in different parts of the horn do not appear to have a significant advantage or disadvantage in regard to the availability of potassium. The amniotic fluid and vascular splanchnopleur contained only small amounts of radioactivity. The labyrinthine placenta was uniformly active throughout. The potassium content of the labyrinthine placenta, pregnant and nonpregnant uterus, has been determined.
On the Significance of the Neuromeres and Similar Structures in Vertebrate Embryos
Authors: Källén Bengt
Published: April 2021

The relation between certain transverse bulges of the wall of the central nervous system (proneuromeres, neuromeres, and ‘transversal bands’ of migration areas) and proliferative processes was studied. Embryos with early migration areas were treated with colchicine and in this way the proliferative activity was changed. The number of cells in mitosis is increased by the colchicine and as a result of this the depth of the bulges is increased. The normally very shallow ventricular furrows come to resemble neuromeric furrows. The occurrence of the bulges in normal development is therefore probably the result of local increases of proliferative activity, i.e. of the formation of proliferation centres. It is suggested that all three systems of transverse bulges (proneuromeres, neuromeres, and ‘transversal bands’) represent successive activations of transverse proliferation centres.
Identification of embryonic cell lineages in histological sections ofM. musculus ↔M. carolichimaeras
Authors: Rossant J. , Vijh M. , Siracusa L. D. , Chapman V. M.
Published: April 2021

An in situ cell marker system has been developed which allows identification of Mus caroli and Mus musculus cells in interspecific chimaeras. A radioactively labelled, cloned DNA probe to M. musculus satellite DNA was hybridized in situ to sections of M. musculus and M. caroli adult tissues. Autoradiography revealed high levels of hybridization to the nuclei of M. musculus cells, but little or no label bound to M. caroli cells. The DNA probe could also distinguish M. musculus and M. caroli cells in the same tissue section. Patches of labelled and unlabelled cells were clearly identified in sections of adult chimaeric tissues and also in the embryonic ectoderm of 6-5-day embryonic chimaeras.

The ability to recognize M. musculus and M. caroli cells in sections of chimaeras should provide a powerful new tool in analyses of cell lineages in both embryonic and adult mouse chimaeras. The marker system has several advantages over other marker systems so far developed, the most important of which is its ubiquity. Since it is a nuclear marker, only cells without nuclei should be unsuited to its use. The potential of the marker system has been shown by its use in demonstrating directly for the first time the postimplantation derivatives of inner cell mass and trophectoderm in blastocysts ‘reconstituted’ with M. musculus trophectoderm and M. caroli inner cell mass.
Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems
Authors: Pickett-Heaps J. D. , Northcote D. H.
Published: April 2021

The fine-structural changes accompanying mitosis in meristematic cells of the roots and coleoptile tissue of wheat have been studied. A band of microtubules encircling the nucleus appeared in the cytoplasm before the cells entered prophase. These microtubules were oriented at right angles to the direction of the mitotic spindle and were located at the position on the mother cell wall where the future cell plate dividing the daughter cells would have joined it. During prophase the number of microtubules in this preprophase band decreased and eventually disappeared, while microtubules were found to be aligned along the spindle axis. These spindle microtubules appeared as a cone-shaped array of units radiating from the polar zones of the spindle and passing very close tangentially to the nucleus. At late prophase they penetrated the disintegrating nuclear envelope and were seen between the chromosomes. During metaphase and anaphase many microtubules were present running throughout the length of the spindle, and others were found to be attached to chromosomes. Paired sister chromosomes were found joined to microtubules from opposite poles of the spindle. The position and orientation of the lamellae of the endoplasmic reticulum which invaded the spindle from the two poles was closely related to the position and alignment of the microtubules. During the formation of the cell plate vesicles were seen to be collected between the microtubules. As the vesicles fused to form the plate the microtubules were found only at its growing edge, where the vesicles were still being aligned. At the initial stage of its formation the microtubules passed right through the plate, but as it extended they appeared to end at the plate region.

The results of the investigation are discussed in relation to the descriptions of mitosis and cytokinesis based on optical microscopy of living cells.