bluuebunny/crossref_metadata_2025 huggingface dataset:
Atypical Presentation of Rapidly Progressive Cutaneous Metastases of Clear Cell Renal Carcinoma: A Case Report
Authors: Iliescu Carmen Andrada , Beiu Cristina , Racoviță Andreea , Olaru Cristina-Mihaela , Tudose Irina , Vrancianu Andreea , Popa Liliana Gabriela
Published: November 2024

Cutaneous metastases from clear cell renal carcinoma (ccRC) are uncommon and often indicate a poor prognosis. These metastases typically occur on the scalp, face, and trunk, and they can be difficult to diagnose due to their resemblance to benign dermatological tumors. We report the case of a 56-year-old patient with a history of ccRC (TNM stage 4) who was referred to our dermatology department with two rapidly enlarging, painful lesions on the left jawline and scalp, which had developed one month and one week earlier, respectively. On examination, the lesions appeared as well-defined, round to oval plaques with a central ulceration and a peripheral red rim, suggestive of an inflammatory appearance. Dermoscopic examination revealed a structureless pink to orange pattern, atypical central vessels, and irregular linear vessels in a corona-like arrangement. Despite the patient’s stable oncological treatment for six months, pain management had recently included paracetamol, tramadol, and NSAIDs. The primary presumptive diagnosis was of cutaneous metastasis, considering the patient’s history of metastatic ccRC. However, given the recent initiation of new pharmacological agents, the rapid progression of the cutaneous lesions, and their clinical presentation, alternative differential diagnoses were considered, including drug-induced reactions such as erythema multiforme or fixed drug eruption. A biopsy of the facial lesion revealed immunohistochemical positivity for CD10, CAIX, and PAX8, confirming the diagnosis of metastatic ccRC with sarcomatoid differentiation. Unfortunately, despite continued targeted therapies and palliative care, the patient’s condition deteriorated rapidly, leading to death two months later. This case highlights the potential for extremely rapidly evolving cutaneous metastases from ccRC and their capacity to occasionally mimic atypical drug eruptions. Additionally, it reaffirms the poor prognosis of such metastases, as evidenced by the patient’s death within two months.
High-Risk Biliary Anastomosis During Robotic Pancreaticoduodenectomy: Initial Experience with Biodegradable Biliary Stent
Authors: González-Abós Carolina , Lorenzo Claudia , Rey Samuel , Salgado Francisco , Ausania Fabio
Published: November 2024

Background and Objectives: Biliary fistulas (BFs) occur in approximately 3–8% of patients undergoing pancreaticoduodenectomy (PD), and the bile duct diameter ≤ 5 mm is the most important risk factor. The aim of this study was to evaluate the efficacy of biodegradable biliary stents (BSs) in reducing complications in patients undergoing robotic pancreaticoduodenectomy (RPD) with a bile duct diameter of ≤5 mm. Materials and Methods: A retrospective single-centre observational study was conducted. Patients undergoing RPD after the completion of the robotic biliary anastomosis learning curve were included in this study. Only patients with a bile duct diameter ≤ 5 mm were included in the analysis. A prospectively held database was used. The intraoperative time for biliary anastomosis was extracted from surgical videos. Results: Of 30 patients, 20 received no biliary stent (nBS) and 10 received a biodegradable stent (BS). The decision to use a stent was based on product availability. The median operative time for biliary anastomosis was significantly shorter in the BS group compared to the nBS group, at 15 min versus 24 min (p < 0.001). Three patients in the nBS group developed a BF, whereas none were observed in the BS group. No stent migration was observed in any of the patients. Conclusions: The use of biodegradable biliary stents in high-risk biliary anastomosis in RPD appears to effectively reduce the incidence of BFs and may serve as a viable strategy to mitigate early biliary complications. The use of biodegradable stents facilitates a faster and easier biliary anastomosis. These findings suggest a potential benefit of using biodegradable stents in complex biliary reconstruction. However, larger studies are needed to confirm these results.
Bupropion Increased More than Five Times the Systemic Exposure to Aripiprazole: An In Vivo Study in Wistar albino Rats
Authors: Ciocotișan Iulia-Maria , Muntean Dana Maria , Vlase Laurian
Published: November 2024

Background/Objectives: In psychiatric disorders, antipsychotics and antidepressant medication are often administered together. Aripiprazole, a third-generation antipsychotic drug, is extensively metabolized by CYP2D6 and CYP3A4 isoenzymes, while bupropion, used in depressive disorders, is known as a moderate or strong CYP2D6 enzyme inhibitor. This in vivo experiment aimed to assess the presence of a pharmacokinetic drug interaction between aripiprazole and bupropion and its magnitude on the systemic exposure of aripiprazole. Methods: 24 healthy Wistar albino male rats were included in two study groups. A single dose of 8 mg/kg aripiprazole was given to rats in the reference group, while the test group received repeated doses of bupropion for 6 days, followed by a single dose of aripiprazole. An LC-MS/MS method was developed for the concomitant quantification of aripiprazole and its active metabolite, dehydroaripiprazole, and non-compartmental analysis was employed to assess their pharmacokinetic parameters. Results: The mean AUC0-∞ of aripiprazole increased 5.65-fold (1117.34 ± 931.41 vs. 6311.66 ± 2978.71 hr·ng/mL), the mean Cmax increased by 96.76% and the apparent systemic clearance decreased over 9-fold after bupropion repeated doses. The exposure to aripiprazole’s active metabolite increased as well, having a 4-fold increase in the mean AUC0–∞ (from 461.13 ± 339.82 to 1878.66 ± 1446.91 hr·ng/mL) and a 2-fold increase in the mean Cmax. Conclusions: The total exposure to the aripiprazole parent compound and active moiety significantly increased after bupropion pretreatment in this preclinical in vivo experiment. Clinical studies should further establish the significance of this interaction in humans.
Topic Editorial on Flexible Electronics
Authors: Xia Meili , Shi Qiongfeng
Published: November 2024

Fields such as the Internet of Things (IoT), smart healthcare, and intelligent manufacturing are at the forefront of technological advancement, involving the extensive deployment of numerous sophisticated electronic systems and devices [...]
Energy Efficient Multi-Active/Multi-Passive Antenna Arrays for Portable Access Points
Authors: Tariq Muhammad Haroon , Zhang Shuai , Masouros Christos , Papadias Constantinos B.
Published: November 2024

This article is about better wireless network connectivity. The main goal is to provide wireless service to several use cases and scenarios that may not be adequately covered today. Some of the considered scenarios are home connectivity, street-based infrastructure, emergency situations, disaster areas, special event areas, and remote areas that suffer from problematic/inadequate network and possibly power infrastructure. A target system that we consider for such scenarios is that of an energy-efficient self-backhauled base station (also called a “portable access point—PAP”) that is mounted on a drone to aid/expand the land-based network. For the wireless backhaul link of the PAP, as well as for the fronthaul of the street-mounted base station, we consider newly built multi-active/multi-passive parasitic antenna arrays (MAMPs). These antenna systems lead to increased range/signal strength with low hardware complexity and power needs. This is due to their reduced number of radio frequency chains, which decreases the cost and weight of the base station system. MAMPs can show a performance close to traditional multiple input/multiple output (MIMO) systems that use as many antenna elements as RF chains and to phased arrays. They can produce a directional beam in any desired direction with higher gain and narrow beamwidth by just tuning the load values of the parasitic elements. The MAMP is designed based on radiation conditions which were produced during the research to ensure that the radiation properties of the array were good.
Alteration in the Gut Microbiota of Chickens Resistant to Eimeria tenella Infection
Authors: Qiao Yu , Feng Qian , Wang Qingjie , Zhao Qiping , Zhu Shunhai , Zhao Fanghe , Wang Zhongchuang , Zhang Ruiting , Wang Jinwen , Yu Yu , Han Hongyu , Dong Hui
Published: November 2024

Avian coccidiosis, caused by several species of Eimeria, is a widespread and economically important poultry disease that inflicts severe losses in the poultry industry. Understanding the interplay between Eimeria and gut microbiota is critical for controlling coccidiosis and developing innovative treatments to ensure good poultry health. In the present study, chickens were immunized six times with a low dose of Eimeria tenella, resulting in complete immunity against Eimeria infection. The results of fecal microbiota transplantation showed that the gut microbiota of immunized chickens induced a certain degree of resistance to coccidial infection. To investigate the types of intestinal microbiota involved in the development of resistance to Eimeria, the intestinal contents and fecal samples from both immunized and unimmunized groups were collected for 16S rRNA gene sequencing. The results showed that, at the genus level, the abundance of the Eubacterium coprostanoligenes group, Erysipelatoclostridium, Shuttleworthia, and Colidextribacter was significantly increased in the intestinal content of immunized chickens, whereas the abundance of Eisenbergiella was significantly decreased. In fecal samples, the abundance of Clostridiaceae and Muribaculaceae significantly increased, whereas that of Bacillales significantly decreased. These findings will help to elucidate the interactions between E. tenella and the gut microbiota of chickens, providing a basis for isolating E. tenella-resistant strains from the gut microbiome and developing new vaccines against coccidiosis.
Prevalence of Babesia microti Co-Infection with Other Tick-Borne Pathogens in Pennsylvania
Authors: Nijjar Lovepreet S. , Schwartz Sarah , Koon Koon Destiny Sample , Marin Samantha M. , Jimenez Mollie E. , Williams Trevor , Chinnici Nicole
Published: November 2024

Babesia microti is a protozoan that infects red blood cells, causing hemolytic anemia and flu-like symptoms in humans. Understanding co-infections is crucial for the better diagnosis, treatment, and management of tick-borne diseases. This study examined the prevalence of Babesia microti co-infection with other prevalent tick-borne pathogens in Pennsylvania. The dataset acquired from the Dr. Jane Huffman Wildlife Genetics Institute included passive surveillance data from Ixodes spp. from 2021 to 2023. Submitted ticks were screened for tick-borne pathogens using species-specific TaqMan qPCR. Of the 793 B. microti-positive ticks pulled for analysis, 65.0% were co-infected with other pathogens (n = 516). Notably, 60.9% of the B. microti-positive ticks were co-infected with Borrelia burgdorferi, 10.2% with Anaplasma phagocytophilum Ap-ha, and 7.5% carried a triple co-infection with B. burgdorferi and A. phagocytophilum Ap-ha. The rates of B. microti infection and its co-infections are on the rise, with patterns observed in Pennsylvania and other regions of the USA. While other studies have collected both nymphal and adult ticks to screen for co-infections in Pennsylvania, our study stood out as a unique contribution to the field by focusing exclusively on B. microti-positive ticks. The continued monitoring of tick-borne co-infections is vital to prevent misdiagnosis and ensure effective treatment regimens.
Effect of the Reactor Material on the Reforming of Primary Syngas
Authors: Bezerra Silva Claudia , Lugo-Pimentel Michael , Ceballos Carlos M. , Lavoie Jean-Michel
Published: October 2024

Syngas, mostly hydrogen and carbon monoxide, has traditionally been produced from coal and natural gas, with biomass gasification later emerging as a renewable process. It is widely used in fuel synthesis through the Fischer–Tropsch (FT) process, where the H2/CO ratio is crucial in determining product efficiency and quality. In this sense, this study aimed to reform an emulated syngas resulting from the supercritical water gasification of biomass, tailoring it to meet the H2/CO ratio required for FT synthesis. Conditions resembling dry reforming were applied, using temperatures from 600 to 950 °C and steel wool as a catalyst. Additionally, the effects of Inconel and stainless steel as reactor materials on syngas reforming were investigated. When Inconel was used, H2/CO ratios ranged between 1.04 and 1.84 with steel wool and 1.28 and 1.67 without. When comparing reactions without steel wool performed either in the Inconel or the stainless steel reactors, those using Inconel consistently outperformed the stainless steel ones, achieving CH4 and CO2 conversions up to 95% and 76%, respectively, versus 0% and 39% with stainless steel. It was concluded that the Inconel reactor exhibited catalytic properties due to its high nickel content and specific oxides.
Roseoside Is a Bioactive Compound in Kirengeshoma koreana Nakai Extract with Potent In Vitro Antiviral Activity Against Hepatitis C Virus
Authors: Lee Jun-Kyu , Choi Ji-Wan , Park InWha , Kim Na-Eun , Kwon Hak Cheol , Kwon Jaeyoung , Song Yoon-Jae
Published: October 2024

Hepatitis C virus (HCV) is a pathogen that causes cirrhosis and hepatocellular carcinoma through chronic hepatitis C. This study focused on the anti-HCV activity of a 70% ethanol extract of Kirengeshoma koreana Nakai (KKE) and its bioactive chemical constituent(s). The KKE and its n-butanol (n-BuOH) fraction induced a significant reduction in HCV RNA levels without inducing cytotoxicity. A high-performance liquid chromatography–mass spectrometry (HPLC-MS) analysis revealed the presence of roseoside in the n-butanol fraction of the KKE, which inhibited HCV RNA replication in a concentration- and time-dependent manner without exerting cytotoxicity. Consistent with in silico molecular docking analysis data, roseoside targets and inhibits HCV NS5A/B replicase. Collectively, our findings demonstrate that roseoside is a chemical constituent in KKE that interferes with HCV replication by targeting NS5A/B replicase.
Recent Advances in Therapeutics for the Treatment of Alzheimer’s Disease
Authors: Thawabteh Amin Mahmood , Ghanem Aseel Wasel , AbuMadi Sara , Thaher Dania , Jaghama Weam , Karaman Donia , Karaman Rafik
Published: October 2024

The most prevalent chronic neurodegenerative illness in the world is Alzheimer’s disease (AD). It results in mental symptoms including behavioral abnormalities and cognitive impairment, which have a substantial financial and psychological impact on the relatives of the patients. The review discusses various pathophysiological mechanisms contributing to AD, including amyloid beta, tau protein, inflammation, and other factors, while emphasizing the need for effective disease-modifying therapeutics that alter disease progression rather than merely alleviating symptoms. This review mainly covers medications that are now being studied in clinical trials or recently approved by the FDA that fall under the disease-modifying treatment (DMT) category, which alters the progression of the disease by targeting underlying biological mechanisms rather than merely alleviating symptoms. DMTs focus on improving patient outcomes by slowing cognitive decline, enhancing neuroprotection, and supporting neurogenesis. Additionally, the review covers amyloid-targeting therapies, tau-targeting therapies, neuroprotective therapies, and others. This evaluation specifically looked at studies on FDA-approved novel DMTs in Phase II or III development that were carried out between 2021 and 2024. A thorough review of the US government database identified clinical trials of biologics and small molecule drugs for 14 agents in Phase I, 34 in Phase II, and 11 in Phase III that might be completed by 2028.
Practical Remediation of Hg-Contaminated Groundwater by MoS2: Batch and Column Tests
Authors: Wang Haifeng , Wei Shuai , Huang Shuai , Liu Wei , Wang Zongwu
Published: October 2024

Trace mercury contamination in groundwater poses a serious threat to ecological systems and human health. The kinetics and isotherms of MoS2 (MS) for Hg removal were studied in batch tests under an unfavorable high salinity and low mercury environment. Flower-like MS with nanosheets can effectively remove Hg in the groundwater matrix, with a shorter equilibrium time (3 h), superior removal efficiency (94.26%), excellent distribution coefficient (5.69 × 106 mL g−1), and higher maximum adsorption capacity (926.10 ± 165.25 mg g−1). Furthermore, the Adams-Bohart model (R2 = 0.9052–0.9416) can accurately describe the dynamic interception process of the initial stage (≤40 PVs), and the Yan model (R2 = 0.9765−0.9941) depicts the whole process (140 PVs) of MS in a fixed column well. A higher dosage of m, but lower C0 and νp facilitate the interception efficiency in column tests. Based on the characterizations of X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM), which were used to simultaneously consider the species of Hg and the groundwater matrix, surface complexation, electrostatic attraction, ion exchange, and precipitation is a plausible interfacial adsorption mechanism of MS for mercury. The excellent performance demonstrates that MS with nanosheets is a promising candidate for the PRB remediation of trace Hg in saline groundwater.
Interaction Dynamics of Plant-Specific Insert Domains from Cynara cardunculus: A Study of Homo- and Heterodimer Formation
Authors: Sampaio Miguel , Santos Sofia , Jesus Ana Marta , Pissarra José , Di Sansebastiano Gian Pietro , Alvim Jonas , Pereira Cláudia
Published: October 2024

Plant aspartic proteinases (APs) from Cynara cardunculus feature unique plant-specific insert (PSI) domains, which serve as essential vacuolar sorting determinants, mediating the transport of proteins to the vacuole. Although their role in vacuolar trafficking is well established, the exact molecular mechanisms that regulate PSI interactions and functions remain largely unknown. This study explores the ability of PSI A and PSI B to form homo- and heterodimers using a combination of pull-down assays, the mating-based split-ubiquitin system (mbSUS), and FRET-FLIM analyses. Pull-down assays provided preliminary evidence of potential PSI homo- and heterodimer formation. This was conclusively validated by the more robust in vivo mbSUS and FRET-FLIM assays, which clearly demonstrated the formation of both homo- and heterodimers between PSI A and PSI B within cellular environments. These findings suggest that PSI dimerization is related to their broader functional role, particularly in protein trafficking. Results open new avenues for future research to explore the full extent of PSI dimerization and its implications in plant cellular processes.
Impact of Button Mushroom Stem Residue as a Functional Ingredient for Improving Nutritional Characteristics of Pizza Dough
Authors: Gallardo Miguel A. , Vieira Júnior Wagner G. , Martínez-Navarro María Esther , Álvarez-Ortí Manuel , Zied Diego C. , Pardo José E.
Published: October 2024

In this study, the formulation of doughs was investigated using varying percentages of Agaricus bisporus flour, with the aim of utilizing mushroom stem fragments, typically considered production waste. The stem residues were collected from a mushroom cultivation facility, cleaned, and washed to remove impurities. The material was then subjected to two different drying methods: conventional dehydration and freeze-drying. After drying, the material was ground to produce mushroom flour. Doughs were formulated with different proportions of this flour and analyzed for texture profile, color, nutritional value, phenolic content, antioxidant activity, and sensory characteristics. The inclusion of mushroom flour resulted in darker doughs, particularly when the flour was obtained through conventional dehydration due to oxidation processes. This substitution also affected texture parameters, leading to increased hardness and reduced elasticity in most treatments compared to the control sample. In addition, cohesiveness progressively decreased from 0.35 in the control to 0.14 in the sample made with 100% dehydrated flour and 0.20 in the sample made with 100% freeze-dried flour, resulting in brittle doughs. The most significant impact on nutritional value was an increase in protein, fat, and dietary fiber levels, reaching values over 5% of crude fiber in the sample to which 50% of dehydrated mushroom flour was added. Additionally, mushroom flours exhibited a high proportion of phenolic compounds, reaching values near 700 mg gallic acid/100 g in the flour from freeze-dried samples and 320 mg gallic acid/100 g in the flour from dehydrated samples. These values reflect a higher content of phenolic compounds in products made with mushroom flours and an increased antioxidant capacity compared to the control sample. Sensory evaluation showed that the texture remained unaffected; however, flavor perception was altered at a 50% mushroom flour substitution. In terms of external appearance, only the 25% freeze-dried mushroom flour formulation was statistically similar to the control, while all other treatments were rated lower.
Discovery of Cyclic Peptide Inhibitors Targeted on TNFα-TNFR1 from Computational Design and Bioactivity Verification
Authors: Zhang Jiangnan , Zhao Huijian , Zhou Qianqian , Yang Xiaoyue , Qi Haoran , Zhao Yongxing , Yang Longhua
Published: October 2024

Activating tumor necrosis factor receptor 1 (TNFR1) with tumor necrosis factor alpha (TNFα) is one of the key pathological mechanisms resulting in the exacerbation of rheumatoid arthritis (RA) immune response. Despite various types of drugs being available for the treatment of RA, a series of shortcomings still limits their application. Therefore, developing novel peptide drugs that target TNFα-TNFR1 interaction is expected to expand therapeutic drug options. In this study, the detailed interaction mechanism between TNFα and TNFR1 was elucidated, based on which, a series of linear peptides were initially designed. To overcome its large conformational flexibility, two different head-to-tail cyclization strategies were adopted by adding a proline-glycine (GP) or cysteine-cysteine (CC) to form an amide or disulfide bond between the N-C terminal. The results indicate that two cyclic peptides, R1_CC4 and α_CC8, exhibit the strongest binding free energies. α_CC8 was selected for further optimization using virtual mutations through in vitro activity and toxicity experiments due to its optimal biological activity. The L16R mutant was screened, and its binding affinity to TNFR1 was validated using ELISA assays. This study designed a novel cyclic peptide structure with potential anti-inflammatory properties, possibly bringing an additional choice for the treatment of RA in the future.
Crystallization and Optical Behaviour of Nanocomposite Sol-Gel TiO2:Ag Films
Authors: Ivanova Tatyana , Harizanova Antoaneta , Koutzarova Tatyana , Closset Raphael
Published: October 2024

Sol-gel spin coating method was employed for depositing TiO2 and Ag-doped TiO2 films. The effects of Ag doping and the annealing temperatures (300–600 °C) were studied with respect to their structural, morphological, vibrational, and optical properties. Field Emission Scanning Electron microscopy (FESEM) investigation exhibited the grained, compact structures of TiO2-based films. Ag incorporation resulted in a rougher film surface. X-ray diffraction (XRD) results confirmed the formation of Ag nanoparticles and AgO phase, along with anatase and rutile TiO2, strongly depending on Ag concentration and technological conditions. AgO fraction diminished after high temperature annealing above 500 °C. The vibrational properties were characterized by Fourier Transform Infrared (FTIR) spectroscopy. It was found that silver presence induced changes in IR bands of TiO2 films. UV–VIS spectroscopy revealed that the embedment of Ag NPs in titania matrix resulted in higher absorbance across the visible spectral range due to local surface plasmon resonance (LSPR). Ag doping reduced the optical band gap of sol-gel TiO2 films. The optical and plasmonic modifications of TiO2:Ag thin films by the number of layers and different technological conditions (thermal and UV treatment) are discussed.
The Molecular Design of a Macrocycle Descaling Agent Based on Azacrown and the Mechanism of Barium Sulfate Scale Removal
Authors: Wu Da , Liu Dexin , Shi Minghua , Wang Jiaqiang , Zhao Han , Dong Yeliang
Published: October 2024

The formation of barium sulfate scale is a persistent and formidable challenge across various industrial processes. In order to effectively mitigate this problem, this study proposed the development of an innovative azacrown ether-based macrocycle descaling agent. Using density functional theory, an in-depth analysis of the surface energy of different barium sulfate crystal facets was carried out, together with a detailed investigation into the adsorption properties of the functional groups on the (001) surface. A further comprehensive investigation was carried out to determine how changes in the nitrogen and oxygen atoms in the crown ether framework influence its adsorption affinity to barium ions. In addition, a detailed analysis was carried out to elucidate the molecular interactions between crown ethers with pyridine carboxylic acid side chains and barium sulfate. The newly developed decalcifying macrocycle descaling agent exhibited superior adsorption performance, achieving an adsorption energy for barium ions approximately −4.1512 ev higher than that of conventional DTPA decalcifiers. This remarkable improvement is mainly attributed to the pivotal role of electrostatic forces in the coordination process between the macrocycle descaling agent and barium ions, with an electrostatic potential value reaching −143.37 kcal/mol. This discovery not only introduces a novel approach to the removal of barium sulfate scale but also highlights the significant potential of macrocycle chemistry in industrial applications.
Manuka Essential Oil Triggers Apoptosis and Activation of c-Jun N-Terminal Kinase in Fibroblasts and Fibrosarcoma Cells
Authors: Bass Noa I. , Parekh Mruga Y. , Satyal Prabodh , Soni Subah , Jacob Jive A. , Mack James P. , Lobo Dorothy E.
Published: October 2024

Manuka essential oil has long been used in traditional medicine, though the effects of the oil on cancer cells have limited studies. The goal of this project was to treat cancer cell lines with manuka essential oil at different concentrations and to ascertain the effects on the cell proliferation of normal fibroblast (CUA-4) and on fibrosarcoma (HT-1080) cells. Cell lines were grown on 24-well plates, and subconfluent cultures were treated with varying concentrations of manuka oil for 24 h. The effect of the oil on proliferation and viability was measured through direct cell counting using trypan blue dye exclusion and through the use of an MTT assay. As the concentration of oil increased, proliferation of all cell lines tested decreased with increasing dosage, concurrently with a decrease in MTT activity. To determine if the decrease in cell numbers observed from manuka oil treatment is the result of apoptosis, PARP cleavage assays were performed, confirming that the treatment caused apoptosis in both normal fibroblasts and fibrosarcoma cells. The stress-activated MAPK protein, JNK, was activated by manuka essential oil treatment, occurring synergistically with a decrease in MKP-1 expression.
Tracking Long-Lived Free Radicals in Dandelion Caused by Air Pollution Using Electron Paramagnetic Resonance Spectroscopy
Authors: Stefaniuk Ireneusz , Cieniek Bogumił , Ćwik Agata , Kluska Katarzyna , Kasprzyk Idalia
Published: November 2024

Studies on particulate air pollution indicate that a new type of pollutant should be considered from mainly fossil fuel combustion and automobile exhaust emissions, i.e., environmentally persistent free radicals. These radicals, ubiquitous in the environment, have a long life span and are capable of producing harmful reactive oxygen species. Samples of dandelion were collected in 2020 and 2021 in spring and late summer. Roots, leaves, flower stalks, and inflorescences of Taraxacum sp. were collected from six sites with three plants each, along with monitoring of particulate matter air pollution. Four sites were located at streets with heavy traffic and two were control sites in the rural part of the city. The free radical content in each part of the plant was measured by electron paramagnetic resonance. The leaf was selected as the most appropriate part of the plant for the measurement of carbon-derived free radicals. The geff value and the total number of spins were calculated. Relationships were found between location, season, and measurements. The electron paramagnetic resonance spectrum consists of at least two components, which can be attributed to C-type radicals and mixed C + O radicals. Their increase in numbers in the fall seasons, compared to the spring seasons, is also noticeable. It has also been observed that leaves collected in autumn have a higher geff value, which is probably related to the higher amount of oxygen- and carbon-derived free radicals.
Blood Cell Membrane-Coated Nanomaterials as a Versatile Biomimetic Nanoplatform for Antitumor Applications
Authors: Shen Hanchun , Ouyang Yongliang , Zhang Liang , Li Jing , Wang Shige
Published: November 2024

The application of nanomaterials in tumor therapy is increasingly widespread, offering more possibilities for enhanced tumor therapy. However, the unclear biological distribution and metabolism of nanomaterials may lead to immune rejection or inflammatory reactions, posing numerous challenges to their clinical translation. The rich diversity and multifaceted functions of blood cells offer promising biological avenues for enhancing the application of nanoparticles in cancer therapy. Blood cell membranes, being made of naturally found components in the body, exhibit significant biocompatibility, which can reduce the body’s immune rejection response, extend the drug’s residence time in the bloodstream, and enhance its bioavailability. Integrating blood cell membranes with nanomaterials enhances tumor therapy by improving targeted delivery, prolonging circulation time, and evading immune responses. This review summarizes recent advancements in the application of blood cell membrane-coated nanomaterials for antitumor therapy, with a particular focus on their use in photodynamic and photothermal treatments. Additionally, it explores their potential for synergistic effects when combined with other therapeutic modalities.
Synthesis of Composition-Tunable Ag-Cu Bimetallic Nanoparticles Through Plasma-Driven Solution Electrolysis
Authors: Xu Chi , Andaraarachchi Himashi P. , Kortshagen Uwe R.
Published: November 2024

Bimetallic nanomaterials have shown great potential across various fields of application. However, the synthesis of many bimetallic particles can be challenging due to the immiscibility of their constituent metals. In this study, we present a synthetic strategy to produce compositionally tunable silver–copper (Ag-Cu) bimetallic nanoparticles using plasma-driven liquid surface chemistry. By using a low-pressure nonthermal radiofrequency (RF) plasma that interacts with an Ag-Cu precursor solution at varying electrode distances, we identified that the reduction of Ag and Cu salts is governed by two “orthogonal” parameters. The reduction of Cu2+ is primarily influenced by plasma electrons, whereas UV photons play a key role in the reduction of Ag+. Consequently, by adjusting the electrode distance and the precursor ratios in the plasma–liquid system, we could control the composition of Ag-Cu bimetallic nanoparticles over a wide range.
Capsaicin Exerts Antitumor Activity in Mesothelioma Cells
Authors: Andretta Emanuela , Costa Aurora , Ventura Elisa , Quintiliani Massimiliano , Damiano Sara , Giordano Antonio , Morrione Andrea , Ciarcia Roberto
Published: November 2024

Background/Objectives: Mesothelioma is an aggressive cancer with limited treatment options. Mesothelioma therapy often involves a multimodal approach including surgery, radiotherapy and chemotherapy. However, the prognosis for patients remains poor. Difficult diagnosis, late symptoms when the tumor is in an advanced stage and the onset of chemotherapy resistance make mesothelioma difficult to treat. For this reason, it is essential to discover new pharmacological approaches. Capsaicin (CAPS) is the active compound of chili peppers. Based on CAPS’s anticancer properties on various tumor lines and its chemo-sensitizing action on resistant cells, in this study, we evaluated the effects of CAPS on mesothelioma cells to assess its potential use in mesothelioma therapy. Methods: To evaluate antiproliferative effects of CAPS, we performed MTS assays on various mesothelioma cells, representative of all major mesothelioma subtypes. Transwell migration and wound-healing assays were used to examine the effect of CAPS on mesothelioma cell migration. We also determined the effects of CAPS on oncogenic signaling pathways by assessing the levels of AKT and MAPK activation. Results: In this study, we show that CAPS significantly reduces proliferation of both parental and cisplatin-resistant mesothelioma cells. CAPS promotes S-phase cell cycle arrest and inhibits lateral motility and migration of mesothelioma cells. Accordingly, CAPS suppresses AKT and ERK1/2 activation in MSTO-211H and NCI-H2052 cells. Our results support an antitumor effect of CAPS on cisplatin-resistant mesothelioma cells, suggesting that it may reduce resistance to cisplatin. Conclusions: Our results could pave the way for further studies to evaluate the use of CAPS for mesothelioma treatment.
Optimizing Antitumor Effect of Triple-Negative Breast Cancer via Rosmarinic Acid–β-Cyclodextrin Inclusion Complex
Authors: Li Yuan , Inam Muhammad , Hasan Muhammad Waqqas , Chen Kaixin , Zhang Zhongqian , Zhu Yongcheng , Huang Jiayu , Wu Zhuowen , Chen Wenjie , Li Min
Published: November 2024

Background: Rosmarinic acid (ROS) has gained notable attention for its anticancer potential; however, its limited aqueous solubility hinders its effective delivery and application in pharmaceutical formulations. Methods: To overcome this limitation, an inclusion complex of ROS with β-cyclodextrin (β-CD) was prepared using the recrystallization method. The resultant ROS–β-CD complex was comprehensively characterized by powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). Results: The ROS–β-CD complex showed a significant improvement in the solubility and dissolution profile of ROS, underscoring its potential for enhanced bioavailability and therapeutic efficacy in pharmaceutical applications. In vitro assays were performed to assess the effects on cell viability, proliferation, apoptotic pathways, and 3D spheroid tumor models. Conclusions: The results demonstrated that ROS–β-CD exhibited superior anticancer properties compared to free ROS, effectively reducing the viability and proliferation of the MD-MBA-231 cell line and inducing apoptosis. This research signifies a substantial advancement in developing therapeutic strategies for TNBC, leveraging the distinct properties of the ROS–β-CD inclusion complex.
All-Optical Encryption Controlled by Multiphotonic Absorption in Carbon Nanotubes
Authors: Lopez-Bastida Alexia , Mercado-Zúñiga Cecilia , Bornacelli Jhovani , de la Rosa José Manuel , Torres-Torres Carlos
Published: November 2024

This study presents an all-optical approach based on an XOR logic gate for encryption by interference and the assistance of multiphotonic effects exhibited by carbon nanotubes. We integrate a Michelson interferometer to propose the encryption system. The key innovation lies in the use of multiwalled carbon nanotubes (MWCNT) to control the XOR operation through intensity-dependent nonlinear optical absorption. We introduce control based on nanosecond nonlinear optical absorption in MWCNT. By measuring irradiance propagation through thin-film samples of MWCNT, we demonstrate a threshold-based binary data recording system that is highly resistant to unauthorized access. The combination of interferometric response, MWCNT-based intensity control, and multicriteria decision analysis through nonlinear absorption presents a powerful and versatile approach to optical encryption. This method has the potential to be a base for secure communication systems and optical computing, with possible extensions to biological computing and microbiology. While challenges in power optimization and scaling remain, this research marks a significant step towards advanced, ultrafast encryption systems.
From Flourish to Nourish: Cultivating Soil Health for Sustainable Floriculture
Authors: Zhang Peihua , Zhou Jie , He Di , Yang Yiran , Lu Zhenhong , Yang Chunmei , Zhang Dongdong , Li Fan , Wang Jihua
Published: November 2024

Despite its rapid growth and economic success, the sustainability of the floriculture industry as it is presently conducted is debatable, due to the huge environmental impacts it initiates and incurs. Achieving sustainability requires joint efforts from all stakeholders, a fact that is often neglected in discussions that frequently focus upon economically driven management concerns. This review attempts to raise awareness and collective responsibility among the key practitioners in floriculture by discussing its sustainability in the context of soil health, as soil is the foundation of agriculture systems. Major challenges posed to soil health arise from soil acidification and salinization stimulated by the abusive use of fertilizers. The poisoning of soil biota by pesticide residues and plastic debris due to the excessive application of pesticides and disposal of plastics is another significant issue and concern. The consequence of continuous cropping obstacles are further elucidated by the concept of plant-soil feedback. Based on these challenges, we propose the adoption and implementation of several sustainable practices including breeding stress-resistant and nutrient-efficient cultivars, making sustainable soil management a goal of floriculture production, and the recycling of plastics to overcome and mitigate the decline in soil health. The problems created by flower waste materials are highlighted and efficient treatment by biochar synthesis is suggested. We acknowledge the complexity of developing and implementing the proposed practices in floriculture as there is limited collaboration among the research and operational communities, and the policymakers. Additional research examining the impacts the floriculture industry has upon soils is needed to develop more sustainable production practices that can help resolve the current threats and to bridge the understanding gap between researchers and stakeholders in floriculture.
Identification of Target Gene and Interacting Protein of Two LaSCL6 Alternative Splicing Variants Provides Novel Insights into Larch Somatic Embryogenesis
Authors: Zang Qiao-Lu , Ye Zha-Long , Qi Li-Wang , Li Wan-Feng
Published: November 2024

Somatic embryogenesis is valuable for clonal propagation and genetic improvement, and it also serves as an ideal system for studying plant development mechanisms. In Larix kaempferi, microRNA171 and its target gene L. kaempferi SCARECROW-LIKE6 (LaSCL6), which has two alternative splicing variants, can regulate somatic embryogenesis; however, the underlying molecular mechanism is still unknown. In this study, we overexpressed these two LaSCL6 variants in Oryza sativa and Arabidopsis thaliana and then used the RNA-Seq method to screen genes from O. sativa and A. thaliana, whose expression patterns are related to those of LaSCL6 variants. The screened genes were then used to search L. kaempferi proteins to identify the candidate target genes of LaSCL6. After yeast one-hybrid and dual- luciferase transcriptional activity assays, cytochrome P450, family 89, subfamily A, polypeptide 5 (CYP89A5), and wall-associated receptor kinase-like 20 (WAKL20) were confirmed to be the target genes of LaSCL6-var1; in addition, WAKL20 and UDP-glycosyltransferase 85A3 (UGT85A3) were confirmed to be the target genes of LaSCL6-var2. Moreover, APETALA2-like protein 2, a transcription factor from the AP2/ERF family, was shown to interact with LaSCL6-var1 and LaSCL6-var2. Taken together, our results suggest a regulatory network of miR171-LaSCL6. The findings presented here not only provide novel insights into the regulation of the miR171-LaSCL6 module but also explain the mechanism underlying larch somatic embryogenesis and other biological processes.
Advances in Soybean Genetic Improvement
Authors: Vargas-Almendra Adriana , Ruiz-Medrano Roberto , Núñez-Muñoz Leandro Alberto , Ramírez-Pool José Abrahán , Calderón-Pérez Berenice , Xoconostle-Cázares Beatriz
Published: November 2024

The soybean (Glycine max) is a globally important crop due to its high protein and oil content, which serves as a key resource for human and animal nutrition, as well as bioenergy production. This review assesses recent advancements in soybean genetic improvement by conducting an extensive literature analysis focusing on enhancing resistance to biotic and abiotic stresses, improving nutritional profiles, and optimizing yield. We also describe the progress in breeding techniques, including traditional approaches, marker-assisted selection, and biotechnological innovations such as genetic engineering and genome editing. The development of transgenic soybean cultivars through Agrobacterium-mediated transformation and biolistic methods aims to introduce traits such as herbicide resistance, pest tolerance, and improved oil composition. However, challenges remain, particularly with respect to genotype recalcitrance to transformation, plant regeneration, and regulatory hurdles. In addition, we examined how wild soybean germplasm and polyploidy contribute to expanding genetic diversity as well as the influence of epigenetic processes and microbiome on stress tolerance. These genetic innovations are crucial for addressing the increasing global demand for soybeans, while mitigating the effects of climate change and environmental stressors. The integration of molecular breeding strategies with sustainable agricultural practices offers a pathway for developing more resilient and productive soybean varieties, thereby contributing to global food security and agricultural sustainability.
Isolation of Lessertiosides A and B and Other Metabolites from Lessertia frutescens and Their Neuroprotection Activity
Authors: Ndjoubi Kadidiatou O. , Omoruyi Sylvester I. , Luckay Robert C. , Hussein Ahmed A.
Published: November 2024

Lessertia frutescens (synonym Sutherlandia frutescens) is an important South African medicinal plant used traditionally to treat different human pathologies and is considered an adaptogenic plant. This study sought to isolate compounds from the plant and determine their protective potentials using SH-SY5Y cells and MPP+ (1-methyl-4-phenylpyridinium) to mimic Parkinson’s disease. The phytochemical analysis of a 70% aqueous methanolic extract of L. frutescens leaves resulted in the isolation and identification of 11 pure compounds (1–11), among which compounds 1 and 2 were identified as new metabolites. The new compounds were characterised using IR, UV, NMR, and HRESIMS and were given the trivial names lessertiosides A (1) and B (2). Additionally, the flavonoids 8-methoxyvestitol (7) and mucronulatol (8) were isolated for the first time from the plant. The biological actions show that the isolated compounds had negligible toxicity on SH-SY5Y cells and improved cell viability in the cells exposed to MPP+. Furthermore, as a mechanism of action, the compounds could sustain cellular ATP generation and prevent MPP+-induced apoptotic cell death. Our findings provide evidence for the neuroprotective properties of compounds isolated from L. frutescens in MPP+-induced neuronal damage for the first time and create an avenue for these compounds to be further investigated to elucidate their molecular targets.
Diversity of Major Yield Traits and Nutritional Components Among Greenhouse Grown Chickpea (Cicer arietinum L.) Breeding Lines, Landraces, and Cultivars of Different Origins
Authors: Choi Yu-Mi , Yoon Hyemyeong , Shin Myoung-Jae , Lee Sukyeung , Yi Jungyoon , Wang Xiaohan , Desta Kebede Taye
Published: November 2024

This study analyzed the diversity of major yield traits and nutritional components across 122 chickpea breeding lines, cultivars, and landraces of different origins. All parameters showed significant variations, with a variance ranging from 4.61% in days to maturity (DM) to 43.04% in oleic acid. Six accessions, including CP021, CP022, CP026, CP037, CP066, and CP109, outperformed in yield traits and nutritional value. Origin significantly affected all phenotypic traits except total fatty acid contents, with Indian and Ukrainian accessions demonstrating contrasting performances. Most traits, except for the number of seeds per pod (SPP), palmitic acid, and total fatty acid contents, differed significantly among breeding lines, cultivars, and landraces. Breeding lines were the earliest to flower and to mature with average days to flowering (DF) of 50.23 days and DM of 101.50 days. They also had the highest average SPP, number of pods per plant (PPP), total seeds per plant (TSPP), total protein, crude fiber, dietary fiber, linoleic acid, and linolenic acid contents making them preferable for high yield and nutrition. Hierarchical cluster analysis classified the chickpea accessions into seven clusters, showing significant variations in yield traits and nutritional components. Principal component and Pearson’s correlation analyses indicated positive correlations between DM and DF, and between SPP, PPP, and TSPP. Nutritional components also displayed varying associations, with a notable negative correlation between oleic and linoleic acids, the two essential fatty acids. Overall, this study showed the diversity of key phenotypic traits in chickpea breeding lines, cultivars, and landraces of different origins. The significant effects of genotype and origin differences on these traits could be used as a basis for future metabolomics and genomics research.
Induction and Characteristics of Callus Cultures of the Medicinal Plant Tussilago farfara L.
Authors: Bojko Monika , Kędra Magdalena , Adamska Agata , Jakubowska Zuzanna , Tuleja Monika , Myśliwa-Kurdziel Beata
Published: November 2024

Tussilago farfara L. is a traditional medicinal plant valued for its potentially health-promoting metabolites. Its herbal raw material has been recognized and used since ancient times and continues to be widely used in traditional medicine. Introducing this plant species to in vitro cultivation is a challenging task, but once the protocol is developed, such cultures can provide an abundant and inexhaustible source of plant material. In this study, we report the successful induction and growth of vigorous T. farfara callus in vitro. Callus induction was achieved on MS solid media with the combination of indole-3-acetic acid (3 mg/L) and benzyl aminopurine (2 mg/L) in darkness, whereas it appeared inefficient under light conditions and in suspension culture. We present a detailed description of callus growth kinetics, morphological analysis, photosynthetic activity, and biochemical parameters (including protein content and photosynthetic pigments) supported by histological studies. Furthermore, we observed the potential for organogenesis and somatic embryogenesis. This method for the in vitro propagation of T. farfara, along with callus culture maintenance, offers a wide range of applications in pharmacy for the production of valuable metabolites. Moreover, it could benefit the environment by reducing the depletion of natural populations of this species and may serve as an alternative strategy for species conservation in light of global warming.