bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimizing faba bean cultivation: assessing varietal performance in spring and fall
Authors: Torabian Shahram , Zakeri Hossein , Farhangi-Abriz Salar
Published: November 2024

The need to identify specialty crops in Virginia has driven interest in faba beans (Vicia faba L.), which offer potential benefits for crop rotation systems. As a cool-season crop, faba beans can be planted in both fall and spring, providing flexibility in farming schedules. A field study was conducted at Randolph Farm, the Virginia State University Research and Extension Farm, using a completely randomized factorial block design. This study examines the performance of seven faba bean varieties—Ethiopia, NEB247, Aprovecho, EN3, EN47, Windsor and EN45—across three spring (late February, late March and mid April), and three fall (late September, early October and late October) planting dates. Our results demonstrate that both variety and planting date significantly influence the yield and yield components of faba beans. Among the varieties tested, Windsor and EN47 exhibited superior traits across multiple categories, making them preferable for achieving high yields. Conversely, varieties such as EN45, Aprovecho, and NEB247 showed poor performance. Fall planting dates generally resulted in superior growth, yield, and maturity characteristics, underscoring their importance for maximizing faba bean production. We observed that faba beans planted in the fall had 58% more branches, 100% more shoot dry matter, 34% higher 100-seed weight, double the grain yields, and 8% higher harvest index compared to those planted in the spring. To further enhance faba bean production, additional studies are suggested to clarify the physiological relationships between photosynthesis rates and the sink-source dynamics. Furthermore, investigating how planting dates impact the nutrient components of faba beans will provide deeper insights into optimizing their cultivation.
Immortalization of patient-derived lip cells for establishing 3D lip models
Authors: Mansour Farah , Parisi Ludovica , Rihs Silvia , Schnyder Isabelle , La Scala Giorgio C. , Aliu Nijas , Katsaros Christos , Degen Martin
Published: November 2024

Introduction

The lips fulfill various critical physiological roles besides being viewed as a fundamental aesthetic feature contributing to the perception of health and beauty. Therefore, any lip injury, abnormality, or congenital malformation, such as cleft lip, needs special attention in order to restore proper lip function and aesthetics. To achieve this goal, a better understanding of the complex lip anatomy, function, and biology is required, which can only be provided by basic research endeavors. However, the current lack of clinically relevant human lip cells and three-dimensional in vitro lip models, capable of replacing ethically questionable animal experimentations, represents a significant limitation in this area of research.

Methods

To address these limitations, we aimed to pioneer the introduction of immortalized healthy lip- and cleft lip-derived keratinocytes. Primary keratinocytes were isolated from patients’ samples and immortalized by introducing the catalytic domain of telomerase, combined with the targeted knockdown of the cell cycle inhibitor gene, p16INK4A. We then focused on validating the newly established cell lines by comparing their genetic stability and key phenotypic features with their primary keratinocyte counterparts.

Results

The newly established immortalized keratinocyte cell lines demonstrated genetic stability and preserved the main phenotypic characteristics of primary keratinocytes, such as cellular morphology and differentiation capacity. Three-dimensional lip models, generated using these cell lines, proved to be effective and convenient platforms for screening applications, including wound healing and microbial infection of the lip epithelium.

Discussion

The establishment of immortalized keratinocytes derived from healthy and cleft lips represents a significant achievement in lip research. These cell lines and the associated three-dimensional lip models are valuable tools that can be used as convenient screening platforms for various assays in a multitude of lip-related research areas, including dermatology, skin care, wound healing, tissue engineering, and craniofacial anomalies. This work opens new avenues in studying lip abnormalities and provides unique tools for personalized medicine approaches beneficial to patients.
Cdc42 improve SARS-CoV-2 spike protein-induced cellular senescence through activating of Wnt/β-Catenin signaling pathway
Authors: Nong Chunmei , Wu Zhenzhen , Yang Chan , Xu Wei , Luo Linyi , Zhou Jianping , Shen Lihan , Chen Yinghua , Yuan Yaoqin , Hu Guodong
Published: November 2024

Introduction

SARS-CoV-2 infection drove senescent cells and the senescence-associated phenotypes were reported playing roles in disease progression, which contributes to severe COVID-19 and related sequelae. Cdc42 is involved in the regulation of cellular senescence. This study, aimed to investigate the mechanism of the SARS-CoV-2 spike protein regulating cellular senescence through Cdc42.

Methods

K18-hACE2 mice were infected with SARS-CoV-2 Omicron BA.4 or stimulated with spike protein through the airway, the senescent cells and Cdc42 expression in lung tissue were detected. Overexpression of spike protein or exogenous incubation of spike protein was used to simulate the induction of cellular senescence by spike protein. Mechanistic insights into the role of Cdc42 were mainly explored using Western Blot and qRT-PCR.

Results

Spike protein, SARS-CoV-2 infection related, accelerates cell aging by upregulating Cdc42 expression, which furtherly activated the Wnt/β-catenin signaling pathway. Conversely, treatment with ML141 in animal models, a Cdc42 inhibitor, reduced cellular senescence and ameliorated lung injury and inflammation. These results suggest that the upregulation of Cdc42 by the SARS-CoV-2 spike protein induces cellular senescence and enhances β-catenin nuclear translocation.

Discussion

This study provides insights into the mechanisms underlying cellular senescence induced by the SARS-CoV-2 spike protein, offering potential strategies to mitigate the inflammatory response and complications associated with COVID-19 in both the acute and long-term phases.
Optimal scheduling study of green warehousing microgrid based on improved sparrow search algorithm
Authors: Liu Liyang , Zhang Shiyu , Zhang Hongdi , Zhang Ziyan , Liu Yudong
Published: November 2024

Combining green warehousing with wind-solar-storage systems can enhance economic power consumption, energy saving, and emission reduction in green warehousing. To achieve efficient and stable operation of the wind-solar-storage microgrid, this paper proposes an optimal microgrid scheduling strategy based on the Improved Sparrow Algorithm (ISSA). Firstly, a comprehensive benefit model is established based on the economic and environmental benefits of microgrid daily operation. Then, an innovative improved sparrow search algorithm is proposed, which aims to improve the global search and local search capability of the microgrid scheduling problem by introducing improvements such as Logistic-Circle chaotic mapping, Bottle Sea Sheath swarm optimization algorithm, dynamic inertia weights, water wave dynamic factor, and Cauchy-Gaussian variational strategy. Finally, the microgrid optimal scheduling model is solved by the improved sparrow search algorithm and compared with other algorithms. In this paper, Matlab 2016b is used for simulation, and the simulation results show that the ISSA algorithm outperforms other algorithms in terms of solution stability and optimization search capability. Under three modes of operation, ISSA improves the microgrid operation revenue by 6.29%, 5.98%, and 6.31% at least. Therefore, the optimal scheduling scheme obtained based on ISSA improves the daily operating total revenue and the system operation stability of the microgrid.
Research on the optimal scheduling strategy of the integrated energy system of electricity to hydrogen under the stepped carbon trading mechanism
Authors: Xu Minghu , Zhao Deren , Yu Changle , Zhang Su , Wan Jia , Li Wenwen , Liu Hengyu
Published: November 2024

Under the guidance of energy-saving and emission reduction goals, a low-carbon economic operation method for integrated energy systems (IES) has been proposed. This strategy aims to enhance energy utilization efficiency, bolster equipment operational flexibility, and significantly cut down on carbon emissions from the IES. Firstly, a thorough exploration of the two-stage operational framework of Power-to-Gas (P2G) technology is conducted. Electrolyzers, methane reactors, and hydrogen fuel cells (HFCs) are introduced as replacements for traditional P2G equipment, with the objective of harnessing the multiple benefits of hydrogen energy. Secondly, a cogeneration and HFC operational strategy with adjustable heat-to-electricity ratio is introduced to further enhance the IES’s low-carbon and economic performance. Finally, a step-by-step carbon trading mechanism is introduced to effectively steer the IES towards carbon emission control.
Role of circulating T follicular helper subsets following Ty21a immunization and oral challenge with wild type S. Typhi in humans
Authors: Booth Jayaum S. , Rapaka Rekha R. , McArthur Monica A. , Fresnay Stephanie , Darton Thomas C. , Blohmke Christoph J. , Jones Claire , Waddington Claire S. , Levine Myron M. , Pollard Andrew J. , Sztein Marcelo B.
Published: November 2024

Despite decades of intense research, our understanding of the correlates of protection against Salmonella Typhi (S. Typhi) infection and disease remains incomplete. T follicular helper cells (TFH), an important link between cellular and humoral immunity, play an important role in the development and production of high affinity antibodies. While traditional TFH cells reside in germinal centers, circulating TFH (cTFH) (a memory subset of TFH) are present in blood. We used specimens from a typhoid controlled human infection model whereby participants were immunized with Ty21a live attenuated S. Typhi vaccine and then challenged with virulent S. Typhi. Some participants developed typhoid disease (TD) and some did not (NoTD), which allowed us to assess the association of cTFH subsets in the development and prevention of typhoid disease. Of note, the frequencies of cTFH were higher in NoTD than in TD participants, particularly 7 days after challenge. Furthermore, the frequencies of cTFH2 and cTFH17, but not cTFH1 subsets were higher in NoTD than TD participants. However, we observed that ex-vivo expression of activation and homing markers were higher in TD than in NoTD participants, particularly after challenge. Moreover, cTFH subsets produced higher levels of S. Typhi-specific responses (cytokines/chemokines) in both the immunization and challenge phases. Interestingly, unsupervised analysis revealed unique clusters with distinct signatures for each cTFH subset that may play a role in either the development or prevention of typhoid disease. Importantly, we observed associations between frequencies of defined cTFH subsets and anti-S. Typhi antibodies. Taken together, our results suggest that circulating TFH2 and TFH17 subsets might play an important role in the development or prevention of typhoid disease. The contribution of these clusters was found to be distinct in the immunization and/or challenge phases. These results have important implications for vaccines aimed at inducing long-lived protective T cell and antibody responses.
Assessing sepsis-induced immunosuppression to predict positive blood cultures
Authors: Hernández-Jiménez Enrique , Plata-Menchaca Erika P. , Berbel Damaris , López de Egea Guillem , Dastis-Arias Macarena , García-Tejada Laura , Sbraga Fabrizio , Malchair Pierre , García Muñoz Nadia , Larrad Blasco Alejandra , Molina Ramírez Eva , Pérez Fernández Xose , Sabater Riera Joan , Ulsamer Arnau
Published: November 2024

Introduction

Bacteremia is a life-threatening condition that can progress to sepsis and septic shock, leading to significant mortality in the emergency department (ED). The standard diagnostic method, blood culture, is time-consuming and prone to false positives and false negatives. Although not widely accepted, several clinical and artificial intelligence-based algorithms have been recently developed to predict bacteremia. However, these strategies require further identification of new variables to improve their diagnostic accuracy. This study proposes a novel strategy to predict positive blood cultures by assessing sepsis-induced immunosuppression status through endotoxin tolerance assessment.

Methods

Optimal assay conditions have been explored and tested in sepsis-suspected patients meeting the Sepsis-3 criteria. Blood samples were collected at ED admission, and endotoxin (lipopolysaccharide, LPS) challenge was performed to evaluate the innate immune response through cytokine profiling.

Results

Clinical variables, immune cell population biomarkers, and cytokine levels (tumor necrosis factor [TNFα], IL-1β, IL-6, IL-8, and IL-10) were measured. Patients with positive blood cultures exhibited significantly lower TNFα production after LPS challenge than did those with negative blood cultures. The study also included a validation cohort to confirm that the response was consistent.

Discussion

The results of this study highlight the innate immune system immunosuppression state as a critical parameter for sepsis diagnosis. Notably, the present study identified a reduction in monocyte populations and specific cytokine profiles as potential predictive markers. This study showed that the LPS challenge can be used to effectively distinguish between patients with bloodstream infection leading to sepsis and those whose blood cultures are negative, providinga rapid and reliable diagnostic tool to predict positive blood cultures. The potential applicability of these findings could enhance clinical practice in terms of the accuracy and promptness of sepsis diagnosis in the ED, improving patient outcomes through timely and appropriate treatment.
Spleen tyrosine kinase: a novel pharmacological target for sepsis-induced cardiac dysfunction and multi-organ failure
Authors: Verra Chiara , Paulmann Maria Kerstin , Wegener Jamila , Marzani Enrica , Ferreira Alves Gustavo , Collino Massimo , Coldewey Sina Maren , Thiemermann Christoph
Published: November 2024

Sepsis is a systemic condition caused by a dysregulated host response to infection and often associated with excessive release of proinflammatory cytokines resulting in multi-organ failure (MOF), including cardiac dysfunction. Despite a number of effective supportive treatments (e.g. ventilation, dialysis), there are no specific interventions that prevent or reduce MOF in patients with sepsis. To identify possible intervention targets, we re-analyzed the publicly accessible Gene Expression Omnibus accession GSE131761 dataset, which revealed an increased expression of spleen tyrosine kinase (SYK) in the whole blood of septic patients compared to healthy volunteers. This result suggests a potential involvement of SYK in the pathophysiology of sepsis. Thus, we investigated the effects of the highly selective SYK inhibitor PRT062607 (15mg/kg; i.p.) on sepsis-induced cardiac dysfunction and MOF in a clinically-relevant, murine model of sepsis. PRT062607 or vehicle (saline) was administered to 10-weeks-old C57BL/6 mice at 1h after the onset of sepsis induced by cecal ligation and puncture (CLP). Antibiotics (imipenem/cilastatin; 2mg/kg; s.c.) and analgesic (buprenorphine; 0.05mg/kg; i.p.) were administered at 6h and 18h post-CLP. After 24h, cardiac function was assessed in vivo by echocardiography and, after termination of the experiments, serum and cardiac samples were collected to evaluate the effects of SYK inhibition on the systemic release of inflammatory mediators and the degree of organ injury and dysfunction. Our results show that treatment of CLP-mice with PRT062607 significantly reduces systolic and diastolic cardiac dysfunction, renal dysfunction and liver injury compared to CLP-mice treated with vehicle. In addition, the sepsis-induced systemic inflammation (measured as an increase in inflammatory cytokines and chemokines in the serum) and the cardiac activation of NF-kB (IKK) and the NLRP3 inflammasome were significantly reduced in CLP-mice treated with PRT062607. These results demonstrate, for the first time, that SYK inhibition 1h after the onset of sepsis reduces the systemic inflammation, cardiac dysfunction and MOF, suggesting a potential role of the activation of SYK in the pathophysiology of sepsis. Novel therapeutic strategies that inhibit SYK activity may be of benefit in patients with diseases associated with local or systemic inflammation including sepsis.
Germline natural killer cell receptors modulating the T cell response
Authors: Mora-Bitria Laura , Asquith Becca
Published: November 2024

In addition to their central role during innate responses, NK cells regulate adaptive immunity through various mechanisms. A wide array of innate receptors has been involved in the NK cell regulatory function. However, the clinical implications of these regulatory pathways are poorly understood. Here, we review the experimental evidence on the effects of NK cells on T cells and their positive and negative consequences for disease outcome during T cell responses in humans.
Hypoxia-adenosinergic regulation of B cell responses
Authors: Pruitt Layne , Abbott Robert K.
Published: November 2024

Hypoxic microenvironments induce widespread metabolic changes that have been shown to be critical in regulating innate and adaptive immune responses. Hypoxia-induced changes include the generation of extracellular adenosine followed by subsequent signaling through adenosine receptors on immune cells. This evolutionarily conserved “hypoxia-adenosinergic” pathway of hypoxia → extracellular adenosine → adenosine receptor signaling has been shown to be critical in limiting and redirecting T cell responses including in tumor microenvironments and the gut mucosa. However, the question of whether hypoxic microenvironments are involved in the development of B cell responses has remained unexplored until recently. The discovery that germinal centers (GC), the anatomic site in which B cells undergo secondary diversification and affinity maturation, develop a hypoxic microenvironment has sparked new interest in how this evolutionarily conserved pathway affects antibody responses. In this review we will summarize what is known about hypoxia-adenosinergic microenvironments in lymphocyte development and ongoing immune responses. Specific focus will be placed on new developments regarding the role of the hypoxia-adenosinergic pathway in regulating GC development and humoral immunity.
Research progress of the SLFN family in malignant tumors
Authors: Yu Jiale , Guo Zhijuan , Zhang Junyi
Published: November 2024

The Schlafen (SLFN) gene family has emerged as a critical subject of study in recent years, given its involvement in an array of cellular functions such as proliferation, differentiation, immune responses, viral infection inhibition, and DNA replication. Additionally, SLFN genes are linked to chemosensitivity, playing a pivotal role in treating malignant tumors. Human SLFNs comprise three domains: the N-terminal, middle (M), and C-terminal. The N- and C-terminal domains demonstrate nuclease and helicase/ATPase activities, respectively. Meanwhile, the M-domain likely functions as a linker that connects the enzymatic domains of the N- and C-terminals and may engage in interactions with other proteins. This paper aims to present a comprehensive overview of the SLFN family’s structure and sequence, examine its significance in various tumors, and explore its connection with immune infiltrating cells and immune checkpoints. The objective is to assess the potential of SLFNs as vital targets in cancer therapy and propose novel strategies for combined treatment approaches.
Radiotherapy as a metastasis directed therapy for liver oligometastases - comparative analysis between CT-guided interstitial HDR brachytherapy and two SBRT modalities performed on double-layer and single layer LINACs
Authors: Bilski Mateusz , Peszyńska-Piorun Magdalena , Konat-Bąska Katarzyna , Brzozowska Agnieszka , Korab Katarzyna , Wojtyna Ewa , Janiak Przemysław , Ponikowska Julia , Sroka Sylwia , Cisek Paweł , Fijuth Jacek , Kuncman Łukasz
Published: November 2024

Introduction

Surgical resection is gold standard for treatment of liver metastasis, locally ablative techniques including computer tomography (CT)-guided interstitial high-dose-rate (HDR) brachytherapy (CT-BRT) and stereotactic body radiotherapy (SBRT) have gained prominence as alternatives, offering comparable outcomes in selected patients. We aim to compare CT-BRT and SBRT - based on dosimetric analysis.

Material and methods

Patients who underwent CT-BRT for oligometastatic, ≤4cm liver metastases between 2018 and 2024 were eligible. SBRT plans for Halcyon (SBRTh) and TrueBeam (SBRTtb) were prepared virtually. In the CT-BRT group CTV was equal to PTV, for SBRTh and SBRTtb planning, a 5 mm margin was applied to CTV to create PTV. Dose calculation was carried out with the TG-43 algorithm for CT-BRT and Anisotropic Analytical Algorithm for SBRTh and SBRTtb group. Descriptive statistics were used to compare the data. The Wilcoxon pairwise order test was utilized to compare dependent groups.

Results

CT-BRT resulted in a more favorable dose distribution within PTVs for Dmean, D50, and D90, while SBRT showed better results for D98 and V27.5Gy. No significant differences were observed for V25Gy between CT-BRT and SBRTtb, but SBRTh favored over CT-BRT. For OARs, CT-BRT plans showed better values for V5, V10, and V11.6Gy in the uninvolved liver volume. There were no significant differences in dose distribution for the duodenum, bowel, and heart. SBRT modalities performed better in the kidney. CT-BRT had improved dose distribution in the esophagus, great vessels, ribs, skin, spinal cord, and stomach compared to SBRT.

Conclusions

CT-BRT could be a viable alternative to SBRT for certain patients with liver malignancies.
Review of BCL2 inhibitors for the treatment of Waldenström’s macroglobulinaemia and non-IgM lymphoplasmacytic lymphoma
Authors: Tomkins Oliver , D’Sa Shirley
Published: November 2024

Lymphoplasmacytic lymphoma (LPL) is a relatively rare form of indolent B-cell non-Hodgkin’s lymphoma, termed Waldenström’s macroglobulinaemia (WM) in the presence of an IgM paraprotein. Although traditionally treated with combination chemoimmunotherapy, the management is evolving in the era of targeted molecular therapies including Bruton’s tyrosine kinase inhibitors (BTKi). However, intolerance and refractoriness to BTKi mean newer agents are required, and the prognosis of so-called quadruple-refractory patients is poor. BCL2 is an anti-apoptotic, pro-survival protein that promotes lymphoma cell survival. Inhibition of BCL2 using first-in-class agent venetoclax has already altered the treatment paradigm in other conditions, including chronic lymphocytic leukaemia (CLL). In-vivo inhibition of BCL2 has been shown to lead to apoptosis of LPL/WM cells. Five studies have published results on the use of BCL2 inhibitors in WM to date, including oblimersen sodium, venetoclax, and sonrotoclax. Fixed-duration venetoclax resulted in high response rates, but many patients relapsed following the completion of therapy. The combination of venetoclax with ibrutinib resulted in higher and relatively deep response rates, but unexpected deaths due to ventricular events mean this combination cannot be explored. Two pivotal trials are currently evaluating the use of fixed-duration venetoclax, either in combination with rituximab or pirtobrutinib, whereas another multi-arm study is studying the use of continuous sonrotoclax monotherapy for R/R WM or in fixed-duration combination with Zanubrutinib for treatment-naïve patients. The potential role of BCL2 inhibitors in WM/LPL remains under study, with many hopeful that they may provide an additional chemotherapy-free oral alternative for patients requiring treatment. In an indolent condition with existing effective treatment regimens, including CIT and cBTKi, cost-effectiveness and toxicity profile will be key, although an additional treatment modality for quadruple-refractory patients with limited treatment options is urgently required.
Factors affecting the wellbeing of mid-achieving university students: a case study from China
Authors: Zou Dandan , Lin Zitong , Chen Chen , Yu Huiying
Published: November 2024

Background

The study aims to investigate the wellbeing of mid-achieving university students on campus and the factors affecting it. Given that this group represents a large yet often overlooked portion of higher education, the study endeavors to analyze the specific factors affecting their wellbeing to provide insights to foster a more comprehensive and inclusive educational environment.

Methodology

The study adopted a qualitative research method. A total of 30 mid-achieving university students from different departments at Guizhou Medical University in China were interviewed in-depth. The interviews were conducted via the online WeChat platform from 1st March 2024 to 13th September 2024. The semi-structured interviews focused on “moments in campus life that make mid-achieving university students feel happy or joyful.” All interviews were audio-recorded and transcribed into text, which was thoroughly coded and analyzed by the researcher using NVIVO 12 software to comprehensively assess the multiple factors that affect the wellbeing of students.

Results

The wellbeing of mid-achieving university students is affected by a combination of factors, including freedom and autonomy, social interactions, collective activities, campus environment and facilities, and academic achievement.

Conclusion

Enhancing the autonomy of mid-achieving university students can significantly improve their self-efficacy and overall wellbeing. While social interactions and collective activities provide emotional support, they also present interpersonal challenges. Sufficient recreational spaces and a diverse range of food options on campus can help alleviate students’ stress and increase their satisfaction with campus services. Conversely, policies banning motorized bicycles may negatively affect students’ sense of wellbeing. Theoretically, the study contributes to the literature on student wellbeing in higher education psychology, particularly by offering a deeper understanding of the large but often neglected group of mid-achieving students. Practically, the findings emphasize the importance of creating more supportive and responsive educational environments tailored to the needs of these students, thereby facilitating inclusive campus environments and fully harnessing the learning potential of mid-achieving students. The study also proposes specific improvement strategies, such as optimizing campus facilities, enhancing student autonomy, and bolstering social and academic support systems. These measures are expected to directly improve the daily experiences of mid-achieving students and enhance their overall wellbeing.
25 years into research with the Méhes Scale, a comprehensive scale of modern dysmorphology
Authors: Tényi Dalma , Csábi Györgyi , Janszky József , Herold Róbert , Tényi Tamás
Published: November 2024

It has been recognized that subtle, cosmetically insignificant anomalies tend to occur cumulatively in diseases with neurodevelopmental origin. These visible signs of morphogenesis errors are called minor physical anomalies (MPAs), serving as sensitive external markers of abnormal neurodevelopment. After the introduction of the Waldrop Scale, the studies conducted on MPAs in diseases with neurodevelopmental origin gave conflicting results. It has been debated that this discrepancy can be – at least partly – attributed to the use of the Waldrop Scale. Understanding the need of a comprehensive scale of MPAs that also differentiates according to the time of development, Hungarian pediatrician professor of University of Pécs, Károly Méhes developed a scale with 57 items, the only scale differentiating minor malformations from phenogenetic variants. With the use of the Méhes Scale, our research group has been investigating the role of abnormal neurodevelopment in different neuropsychiatric and neurologic disorders since 1997. 25 years into our research, in this review we summarize the results of our 18 research articles on MPAs in different diseases. We have found an increased number of MPAs, especially in the head and mouth region, in patients with schizophrenia, bipolar disorder, Tourette syndrome, autism and many epilepsy syndromes, fortifying the role of abnormal neurodevelopment in these diseases. Moreover, an increased number of MPAs was detected among the first-degree relatives of patients with schizophrenia and bipolar I disorder, supporting the hypothesis about MPAs being endophenotypic trait markers.
Effects of repetitive transcranial magnetic stimulation on inhibitory control in first-episode schizophrenia: behavioral and neural mechanisms
Authors: Yu Sihang , Wang Shuai , Sun Hang
Published: November 2024

Background

Inhibitory control deficits are a core feature of cognitive impairment in schizophrenia, associated with abnormal activation of key brain networks. Repetitive transcranial magnetic stimulation (rTMS) targeting the dorsolateral prefrontal cortex (DLPFC) may help improve inhibitory control, but its specific effects in schizophrenia remain uncertain.

Methods

This study involved 150 participants divided into Real-rTMS, Sham-rTMS, and healthy control groups. Inhibitory control was assessed using the dual-choice oddball task, and task-based functional magnetic resonance imaging (fMRI) was employed to examine neural activity. The Real-rTMS group received active stimulation over the DLPFC, and the Sham group received placebo stimulation.

Results

The Real-rTMS group exhibited significant improvements in both reaction times and accuracy compared to the Sham group, indicating enhanced inhibitory control. fMRI data showed that brain activity in regions such as the cerebellum, insula, thalamus, and prefrontal cortex was normalized in the Real-rTMS group, with activation patterns closely resembling those observed in healthy controls. Additionally, task-based fMRI revealed a restoration and further enhancement of negative activation in regions like the middle frontal gyrus and superior temporal gyrus, which helped reduce cognitive interference from irrelevant stimuli.

Conclusion

rTMS targeting the DLPFC improves inhibitory control in schizophrenia by modulating both positive and negative brain activation patterns. These findings highlight the dual mechanism through which rTMS enhances cognitive control, offering a promising intervention for cognitive deficits in schizophrenia. Future research should explore the long-term effects of this modulation on broader cognitive functions.
Construction and application of the emergency resilience capability evaluation scale and model for grassroots medical workers: a cross-sectional study in Guangzhou, China
Authors: Wu Qiaohui , Wu Linjian , Liang Xueqing , Xu Jun , Liu Li , Huang Zhijie , Ma Zhen
Published: November 2024

Objective

Public health emergencies pose a serious challenge to the ability of medical personnel to respond. We aimed to establish a scientific and effective resilience capability assessment scale for grassroots medical workers and explore the way to improve resilience.

Research design

Developed a resilience ability assessment scale and applied it on the medical personnel who worked in community health service centers for more than 1 year. Related indicators were identified through literature review and the Delphi method. The established scale was given weight using the attribute analytic hierarchy process, and the evaluation model was built through the grey correlation analysis.

Principal findings

A three-level indicator scale was established, which included three first-level indicators [professional quality (weight 0.346), psychological capital (weight 0.614), and emergency attitude (weight 0.040)]. The second-level indicators had 13 contents and were further subdivided into 46 three-level indicators. The overall resilience ability assessment score of 347 medical workers in Guangzhou was only 0.787. However, steady scientific-exercise habits and relative training can help improve their public health emergency resilience ability (p < 0.05).

Conclusion

The establishment of the scale can provide a strategic basis for improving the resilience strength of grass-roots medical personnel. We should encourage this group to form a good habit of exercise, strengthen their crisis awareness by actively organizing theoretical training and practical exercises on public health emergencies, and improve their psychological capital level.

Patient or public contribution

In this study, 347 medical workers from 6 basic medical institutions in Guangzhou participated in the questionnaire.
Exploring and enhancing the accessibility of children's oral health resources (called HABIT) for high risk communities
Authors: Chauhan Amrit , Staples Annalea , Forshaw Eleanor , Zoltie Timothy , Nasser Riffat , Gray-Burrows Kara A. , Day Peter F.
Published: November 2024

Background

Within the city of Bradford in West Yorkshire, South Asian and Eastern European communities have an increased risk of childhood tooth decay, especially among families with Limited English Proficiency. Tooth decay is preventable, with national guidelines advocating home-based behaviours (toothbrushing with fluoride toothpaste and reducing sugar intake). In England, Health Visitors have opportunities to undertake oral health conversations during universal visits for children aged 0–24 months. The HABIT (Health visitors delivering Advice in Britain on Infant Toothbrushing) intervention provides structured oral health conversations, underpinned by complex intervention methodology. A feasibility study found HABIT acceptable to parents, feasible to deliver and led to improvements in home-based behaviours. However, the reach of this original study was limited to those proficient in English. This new study focused on exploring and enhancing the accessibility of the HABIT intervention to parents with Limited English Proficiency.

Method

Twenty-four parents participated in interviews and focus groups, with 21 requesting support from interpreters. Community centres and WhatsApp were used to maximise inclusivity. Interviews and focus groups, followed a topic guide and the “Think Aloud” technique, were professionally transcribed, managed in NVivo, and thematically analysed. Team discussions facilitated analytical rigour. Recruitment continued until data saturation.

Results

Three themes were developed: (1) Navigating linguistic barriers; (2) Engagement through visuals; and (3) Addressing oral health challenges. Parents employed diverse strategies to interpret resources, including Google Translate, as well as family and wider community members. Consequently, the HABIT resources were modified to include simple text, subtitles, and translation tools. Parents highlighted the benefits of shorter oral health messages with clear visuals to help understanding, and this strategy was applied across all resources. Challenges surrounding children's resistance to toothbrushing, high sugar intake within their wider families and communities, and limited dental access were all raised. The HABIT resources were updated to address these challenges.

Conclusion

Collaborative community engagement has enhanced the HABIT resources, enabling access for high-risk communities to preventive oral-health programmes thereby promoting health equity.
Landfill leachate treatment process is transforming and releasing banned per- and polyfluoroalkyl substances to UK water
Authors: Neill Pippa , Megson David
Published: November 2024

Landfills are a known source of PFAS pollution. Many have environmental permits allowing the discharge of treated leachate to controlled waters. In this article we compared leachate data for 17 PFAS from 17 different landfill sites across the UK. The results show that the landfill leachate treatment process (designed to improve water quality) is generating the banned PFAS; PFOA and PFOS. Approximately 80% of locations tested showed an increase in PFOS, with an increase of 1,335% in one sample. The highest concentrations of PFOS and PFOA in treated leachate were 2,460 ng L−1 and 26,900 ng L−1, respectively. When compared against the environmental quality standard of 0.65 ng L−1 for PFOS this leachate could pose a significant concern. Landfill leachate treatment has proven effective for a wide range of different pollutants, but this research shows in several instances that it is not appropriate for PFAS remediation.
Predisposition factors and control strategies of avian pathogenic Escherichia coli in laying hens
Authors: Waliaula Paul K. , Kiarie Elijah G. , Diarra Moussa S.
Published: November 2024

Shift in laying hens housing from conventional cage-based systems to alternatives has impacted their health and performance. Microorganisms colonize young chick in the early stages of their physiological and immune development. These colonizing microbes originate from parent and the environment. Escherichia coli is among the normal gut colonizing bacteria however, some E. coli strains known as avian pathogenic E. coli (APEC), cause local or systemic infections (colibacillosis) responsible of significant economic losses to the poultry industry. Potential APEC strains and other poultry gut microbiota are influenced by several factors such as housing system, and the use of feed additives (prebiotics, probiotics, symbiotic, among others). This review will discuss the status of pullets and layers immunity, gut health, and predisposing factors of colibacillosis. Dietary interventions and some colibacillosis mitigation strategies in pullets and laying hens are reviewed and discussed. With the development of sequencing technologies and the use of feed additives as alternatives to antibiotics, future studies need to understand some of the complex associations between the feed additives, the rearing environment, and their selective pressure on gut microbiota, including E. coli, and their impacts on immune development in pullets and hens.
Case report: A CLCN1 complex variant mutation in exon 15 in a mixed-breed dog with hereditary myotonia
Authors: Eguchi Gabriel Utida , Palumbo Mariana Isa Poci , Cerri Fabrício Moreira , Basso Roberta Martins , Oliveira-Filho José Paes de , Caramalac Silvana Marques , Borges Alexandre Secorun
Published: November 2024

At 4 months of age, a male dog was presented with a complaint of a stiff gait following a startle response. Neurological examination revealed no deficits, but clinical myotonia was easily induced upon requesting the patient to jump. Additionally, myotonia of the upper lip muscles was observed upon manipulation. Hereditary myotonia was suspected, and electromyography confirmed the presence of myotonic potentials. Genetic testing of the myotonic patient identified a complex of mutations, including c.[1636_1639 delins AACGGG] and c.[1644 A>T], both located in exon 15 of the CLCN1 gene leading to the formation of a premature stop codon. Genetic investigations of the mother and four littermates revealed that, except for one littermate who was wild type, all others carried a copy of the mutated gene. To the best of the authors' knowledge, these mutations have not been previously reported.
Optimization Parameters for PLA Through Additive Manufacturing: Taking Mixed Shapes as an Example
Authors: Chen Dyi-Cheng , Chen Yu-Ting
Published: November 2024

In recent years, additive manufacturing has been widely used in industrial, medical, and educational fields. Material extrusion is used in most industries to increase development efficiency and reduce costs. This study used the material extrusion to discuss the print quality of additive manufacturing and optimized the processing parameters based on material properties. Based on the literature, this study summarized the fishbone diagram influencing printing quality. The layer height, nozzle temperature, printing speed, infill pattern, and filling spacing were selected as the control factors of the Taguchi method. An orthogonal array L16 was used for parameter design. The optimal parameters were analyzed using the variance and the response surface method. The results of the study are as follows.
Exploring Core Knowledge in Interdisciplinary Research: Insights from Topic Modeling Analysis
Authors: Wu Shuangyan , Lin Mixin , Ji Mengxiao , Wang Ting
Published: November 2024

Although interdisciplinary research has garnered extensive attention in academia, its core knowledge structure has yet to be systematically explored. To address this gap, this study aims to uncover the underlying core knowledge topics within interdisciplinary research, enabling researchers to gain a deeper understanding of the knowledge framework, improve research efficiency, and offer insights for future inquiries. Based on the Web of Science (WoS) database, this study collected 153 highly cited papers and employed the LDA topic model to identify latent topics and extract the knowledge structure within interdisciplinary research. The findings indicate that the core knowledge topics of interdisciplinary research can be categorized into four major areas: the knowledge framework and social impact of interdisciplinary research, multidisciplinary approaches in cancer treatment and patient care, Covid-19 multidisciplinary care and rehabilitation, and multidisciplinary AI and optimization in industrial applications. Moreover, the study reveals that AI-related interdisciplinary research topics are rapidly emerging. Through an in-depth analysis of these topics, the study discusses potential future directions for interdisciplinary research, including the cultivation and development of interdisciplinary talent, evaluation systems and policy support for interdisciplinary research, international cooperation and interdisciplinary globalization, and AI and interdisciplinary research optimization. This study not only uncovers the core knowledge structure of interdisciplinary research but also demonstrates the effectiveness of the LDA topic model as a data mining tool for revealing key topics and trends, providing practical tools for future research. However, this study has two main limitations: the time lag of highly cited papers and the dynamic evolution of interdisciplinary research. Future research should address these limitations to further enhance the understanding of interdisciplinary research.
A Survey System for Artificial Intelligence-Based Painting Using Generative Adversarial Network Techniques
Authors: Zhang Chaoyang , Li Xiang , Jean Ming-Der
Published: November 2024

The purpose of this paper is to construct an evaluation system for AI painting software based on generative adversarial network (GAN) technology, which optimizes the performance of the related software in terms of functionality, ease of use, system performance, and safety. The results of the questionnaires are statistically analyzed. In addition, an exploratory factor analysis was conducted to extract the data of the study, which were ultimately used to calculate the weight and importance of each index using the fuzzy hierarchical analysis method. This study constructed an evaluation system for AI painting software based on GAN technology, including 16 indicators of functionality, 16 indicators of ease of use, 7 indicators of system performance, and 8 indicators of safety, respectively, whose alpha coefficients were 0.882, 0.962, 0.932, 0.932, and 0.932, respectively. In addition, the accumulated explanatory variances of their coefficients were 84.405%, 84.897%, 84.013%, 72.606%, 73.013%, and 72.606%, respectively. It is clear that the items included in each of the indicators are homogeneous, with a high degree of internal consistency. This paper suggests that the development of AI painting software focusing on functionality, ease of use, system performance, and safety can enhance the market competitiveness of the software.
LncRNA Taurine Up-Regulated 1 Knockout Provides Neuroprotection in Ischemic Stroke Rats by Inhibiting Nuclear-Cytoplasmic Shuttling of HuR
Authors: Shi Xiaocheng , Liu Sha , Zou Yichun , Wu Hengping , Ma Jinyang , Lin Junbin , Zhang Xin
Published: November 2024

Background: Long non-coding RNA taurine-upregulated gene 1 (TUG1) is involved in various cellular processes, but its role in cerebral ischemia–reperfusion injury remains unclear. This study investigated TUG1’s role in regulating the nucleocytoplasmic shuttling of human antigen R (HuR), a key apoptosis regulator under ischemic conditions. Methods: CRISPR-Cas9 technology was used to generate TUG1 knockout Sprague Dawley rats to assess TUG1’s impact on ischemic injury. The infarct area and neuronal apoptosis were evaluated using TUNEL, hematoxylin and eosin (HE), and TTC staining, while behavioral functions were assessed. Immunofluorescence staining with confocal microscopy was employed to examine TUG1-mediated HuR translocation and expression changes in the apoptosis-related proteins COX-2 and Bax. Results: TUG1 knockout rats showed significantly reduced cerebral infarct areas, decreased neuronal apoptosis, and improved neurological functions compared to controls. Immunofluorescence staining revealed that HuR translocation from the nucleus to the cytoplasm was inhibited, leading to decreased COX-2 and Bax expression levels. Conclusions: TUG1 knockout reduces ischemic damage and neuronal apoptosis by inhibiting HuR nucleocytoplasmic shuttling, making TUG1 a potential therapeutic target for ischemic stroke.
Metformin Inhibited GSDME to Suppress M2 Macrophage Pyroptosis and Maintain M2 Phenotype to Mitigate Cisplatin-Induced Intestinal Inflammation
Authors: Jiang Ke , He Qi , Wang Chenhui , Yang Wen , Zhou Changdong , Li Jian , Li Jiangbo , Cui Yuke , Shi Jingqi , Wei Zhenqiao , Jiao Yuanyuan , Bai Ligai , Wang Shengqi , Guo Liang
Published: November 2024

Background: The continuous clinical use of cisplatin is prevented by gastrointestinal toxicity. Methods: Cisplatin was used to treat THP-1-derived macrophages to see its differential effects on different subtypes of macrophages. Wild-type and Gsdme−/− mice models were used to examine the effect of cisplatin and metformin on intestinal inflammation in vivo. The effect of GSDME on macrophage polarization was further confirmed by GSDME knockdown. Results: We found that M2 macrophages, with more cell blebbing and GSDME cleavage, were more sensitive to cisplatin-induced pyroptosis than M1 macrophages. Cisplatin was capable of enhancing the M1 phenotype, which was reversed by GSDME knockdown. GSDME contributed to M1 polarization and GSDME knockdown promoted M2 phenotype via STAT6 activation. Reduced intestinal inflammation and increased M2 macrophage numbers was detected in cisplatin-treated GSDME-knockout mice. Furthermore, metformin alleviated cisplatin-induced intestinal inflammation by reducing M2 pyroptosis and enhancing M2 phenotype through GSDME inhibition. Conclusion: This is the first study to reveal the non-pyroptotic role of GSDME in macrophage polarization, revealing that metformin could be used in combination with cisplatin to reduce intestinal toxicity.