bluuebunny/crossref_metadata_2025 huggingface dataset:
A reformulação do sentido da vida a partir do iluminismo segundo Charles Taylor
Authors: Heldt Michele Borges , Helfer Inácio
Published: April 2018

Nos capítulos 18 e 19 do livro “As fontes do Self”, Charles Taylor busca entender as origens dos principais elementos formadores da identidade contemporânea, o que lhe conduz a uma análise histórica voltada para o fim da idade média e o início da era moderna. Apesar da diversidade dos elementos formadores da identidade moral encontrados nestes períodos, uma questão aparece incomum a todos eles, qual seja: o sentido da vida.  O presente artigo visa discorrer sobre este tema e mostrar, com Taylor, que essa questão ultrapassará a compreensão sobre os desígnios divinos, naturais, racionais e de cunho utilitário, – mas não sem arrastar consigo características herdadas de cada um desses períodos – e culminará em um novo modo de pensar. Esta nova concepção, na verdade, estaria diretamente relacionada com o desenvolvimento da capacidade dos indivíduos em aceitarem suas limitações e, com isso, buscarem entender sua irremediável tendência a atribuir sentido a todas as coisas.
MAPEAMENTO, PROCESSO, CONEXÕES: A CARTOGRAFIA COMO METODOLOGIA DE PESQUISA
Authors: Lemos Cássio Fernandes , Machado Oliveira Andréia
Published: April 2018

O seguinte artigo discorre a respeito da cartografia como metodologia de pesquisa, conforme apresentada por Deleuze e Guattari. O objetivo é explorar suas possibilidades de investigação e formas de construir a pesquisa, pois o mesmo não apresenta etapas ou fases rigidamente estabelecidas para seguir. Assim, através do que é apresentado por autores que estudam a metodologia, como Kastrup, alguns pontos são explorados e comentados. MAPPING, PROCESS, CONNECTIONS: CARTOGRAPHY AS METHODOLOGY FOR RESEARCHAbstract: The following article is about cartography as a research methodology, as presented by Deleuze and Guattari. The objective is to explore its investigation possibilities and means to build research, as there are no steps or phases established to be followed rigidly. Thus, through studying what has been presented by authors who study this methodology, such as Kastrup, some points are explored and commented.
OS TEMPOS DA ARTE FOTOGRÁFICA – UM ENSAIO EM PARIS
Authors: Remião de Macedo Daniela , Vieira da Cunha Eduardo
Published: April 2018

Este trabalho visa um registro da pesquisa em andamento junto ao PPG em Artes Visuais e propõe uma reflexão sobre questões que envolvem a fotografia e o tempo. São abordadas possibilidades da fotografia artística que permitem expandir o tempo do fazer fotográfico, incorporando temporalidades de todo o processo de criação, desde a teatralização fotográfica na pré-produção, a representação do tempo na fotografia, até a seleção e edição das imagens na pós-produção. Por fim, é apresentado o resultado de um ensaio inspirado em Edgar Degas, em uma série de fotografias que compõe uma narrativa, envolvendo a fotografia, a pintura e a dança, onde questões do tempo foram consideradas.THE TIMES OF PHOTOGRAPHIC ART - AN ESSAY IN PARISAbstract: This paper aims to record ongoing Masters research at the Postgraduate Program  in Visual Art/UFRGS, RS, Brazil and proposes reflection on issues involving photography and time. Possibilities of art photography are explored that allow expanding the time of making photography, incorporating temporalities into the whole creative process, from pre-production photographic theatralization, to  representing time in photography, up to the selection and editing of images in post-production. In the end, we present the result as an essay inspired by Edgar Degas, a series of photographs that compose a narrative, involving photography, painting and dance, about which issues of time have been considered.
Testing Significance Testing
Authors: Krueger Joachim I. , Heck Patrick R.
Published: April 2018

The practice of Significance Testing (ST) remains widespread in psychological science despite continual criticism of its flaws and abuses. Using simulation experiments, we address four concerns about ST and for two of these we compare ST’s performance with prominent alternatives. We find the following: First, the p values delivered by ST predict the posterior probability of the tested hypothesis well under many research conditions. Second, low p values support inductive inferences because they are most likely to occur when the tested hypothesis is false. Third, p values track likelihood ratios without raising the uncertainties of relative inference. Fourth, p values predict the replicability of research findings better than confidence intervals do. Given these results, we conclude that p values may be used judiciously as a heuristic tool for inductive inference. Yet, p values cannot bear the full burden of inference. We encourage researchers to be flexible in their selection and use of statistical methods.
Moral Framing and Charitable Donation: Integrating Exploratory Social Media Analyses and Confirmatory Experimentation
Authors: Hoover Joe , Johnson Kate , Boghrati Reihane , Graham Jesse , Dehghani Morteza
Published: April 2018

Do appeals to moral values promote charitable donation during natural disasters? Using Distributed Dictionary Representation, we analyze tweets posted during Hurricane Sandy to explore associations between moral values and charitable donation sentiment. We then derive hypotheses from the observed associations and test these hypotheses across a series of preregistered experiments that investigate the effects of moral framing on perceived donation motivation (Studies 2 & 3), hypothetical donation (Study 4), and real donation behavior (Study 5). Overall, we find consistent positive associations between moral care and loyalty framing with donation sentiment and donation motivation. However, in contrast with people’s perceptions, we also find that moral frames may not actually have reliable effects on charitable donation, as measured by hypothetical indications of donation and real donation behavior. Overall, this work demonstrates that theoretically constrained, exploratory social media analyses can be used to generate viable hypotheses, but also that such approaches should be paired with rigorous controlled experiments.
High prevalence of iodine deficiency in pregnant women living in adequate iodine area
Authors: Mioto Verônica Carneiro Borges , Monteiro Ana Carolina de Castro Nassif Gomes , de Camargo Rosalinda Yossie Asato , Borel Andréia Rodrigues , Catarino Regina Maria , Kobayashi Sergio , Chammas Maria Cristina , Marui Suemi
Published: April 2018

Objectives

Iodine deficiency during pregnancy is associated with obstetric and neonatal adverse outcomes. Serum thyroglobulin (sTg) and thyroid volume (TV) are optional tools to urinary iodine concentration (UIC) for defining iodine status. This cross-sectional study aims to evaluate the iodine status of pregnant women living in iodine-adequate area by spot UIC and correlation with sTg, TV and thyroid function.

Methods

Two hundred and seventy-three pregnant women were evaluated at three trimesters. All had no previous thyroid disease, no iodine supplementation and negative thyroperoxidase and thyroglobulin antibodies. Thyroid function and sTg were measured using electrochemiluminescence immunoassays. TV was determined by ultrasonography; UIC was determined using a modified Sandell–Kolthoff method.

Results

Median UIC was 146 µg/L, being 52% iodine deficient and only 4% excessive. TSH values were 1.50 ± 0.92, 1.50 ± 0.92 and 1.91 ± 0.96 mIU/L, respectively, in each trimester (P = 0.001). sTg did not change significantly during trimesters with median 11.2 ng/mL and only 3.3% had above 40 ng/mL. Mean TV was 9.3 ± 3.4 mL, which positively correlated with body mass index, but not with sTg. Only 4.5% presented with goitre.

When pregnant women were categorized as iodine deficient (UIC < 150 µg/L), adequate (≥150 and <250 µg/L) and excessive (≥250 µg/L), sTg, thyroid hormones and TV at each trimester showed no statistical differences.

Conclusions

Iodine deficiency was detected frequently in pregnant women living in iodine-adequate area. sTg concentration and TV did not correlate to UIC. Our observation also demonstrated that the Brazilian salt-iodization programme prevents deficiency, but does not maintain iodine status within adequate and recommended ranges for pregnant women.
Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo
Authors: Serna Vanida A , Wu Xin , Qiang Wenan , Thomas Justin , Blumenfeld Michael L , Kurita Takeshi
Published: April 2018

Cellular mechanisms of uterine leiomyoma (LM) formation have been studied primarily utilizingin vitromodels. However, recent studies established that the cells growing in the primary cultures ofMED12-mutant LM (MED12-LM) do not carry causal mutations. To improve the accuracy of LM research, we addressed the cellular mechanisms of LM growth and regression utilizing a patient-derived xenograft (PDX) model, which faithfully replicates the patient tumorsin situ. The growth and maintenance of MED12-LMs depend on 17β-estradiol (E2) and progesterone (P4). We determined E2 and P4-activated MAPK and PI3K pathways in PDXs with upregulation of IGF1 and IGF2, suggesting that the hormone actions on MED12-LM are mediated by the IGF pathway. When hormones were removed, MED12-LM PDXs lost approximately 60% of volume within 3 days through reduction in cell size. However, in contrast to general belief, the survival of LM cells was independent of E2 and/or P4, and apoptosis was not involved in the tumor regression. Furthermore, it was postulated that abnormal collagen fibers promote the growth of LMs. However, collagen fibers of actively growing PDXs were well aligned. The disruption of collagen fibers, as found in human LM specimens, occurred only when the volume of PDXs had grown to over 20 times the volume of unstimulated PDXs, indicating disruption is the result of growth not the cause. Hence, this study revises generally accepted theories on the growth and regression of LMs.
Comportamientos proactivos en el trabajo: adaptación y análisis psicométrico de una escala
Authors: Salessi Solana Magalí , Omar Alicia
Published: April 2018

Objetivo: adaptar la Escala de Comportamientos Proactivos de Belschak y Den Hartog, y analizar sus propiedades psicométricas. Método: se realizó un estudio instrumental sobre una muestra no probabilística de 461 trabajadores argentinos. Resultados: análisis factoriales indicaron la pertinencia de un modelo oblicuo de tres factores (SBχ2= 1.79; GFI = .95; CFI = .94; RMSEA = .03) rotulados como comportamientos proactivos orientados a la organización, a los demás, y a uno mismo, respectivamente; con 10 variables observables, invariante según sexo y con adecuada confiabilidad (α = .78; CR = .77; ω= .72). Se obtuvo evidencia de validez convergentediscriminante y concurrente a partir del cómputo del índice AVE (AVE > .50; √AVE < rs ), y de sus correlaciones con medidas de personalidad proactiva (CP orientados a la organización: rs = .34, p < .01; CP orientados a los demás: rs = .30, p < .01; CP orientados a uno mismo: rs = .36, p < .01), satisfacción (CP orientados a la organización: rs = .40, p < .01; CP orientados a los demás: rs = 31, p < .01; CP orientados a uno mismo: rs = .44, p < .01) e implicación laboral (CP orientados a la organización: rs = .36, p < .01; CP orientados a los demás: rs= .25, p < .05; CP orientados a uno mismo: rs = .42, p < .01). Conclusión: se han obtenido evidencias de validez, invarianza factorial y confiabilidad de la versión argentina del instrumento.
Nota sobre un ejemplar inmaduro de Gomphotherium sp. del Mioceno Tardío de Chiapas, México
Authors: Gómez-Pérez Luis E. , Carbot-Chanona Gerardo
Published: April 2018

Se describe un premolar y un fragmento de incisivo superior perteneciente a un gonfoterio longirrostrino, descubiertos en la localidad Puente Ixcán (Mioceno tardío), municipio de Maravilla Tenejapa, en el sureño estado de Chiapas, México. Con base en la morfología, medidas y alcance geográfico del ejemplar lo asignamos al género Gomphotherium. Este registro es el más sureño de Gomphotherium en México y por su cercanía con América Central contribuye al conocimiento de la distribución paleobiogeográfica de los gonfoterios longirrostrinos en esta región.
Comparing Web 2.0 applications for peer feedback in language teaching
Authors: Canham Niki
Published: April 2018

The use of technology for collaborative writing and the delivery of electronic corrective feedback is becoming widespread in educational settings. However, to date, little research has focused on comparing the suitability of different Web 2.0 applications for technology-enhanced peer feedback. This study explores three free-to-use applications: Google Docs, the Sakai Virtual Learning Environment (VLE), and the Sakai Wiki. The study reports on the practicalities and peer reviewers’ experiences of these applications and, most importantly, compares the electronic feedback comments across applications. Seventy-eight English as a Foreign Language (EFL) students at an Austrian university participated in this counterbalanced, repeated-measures study. Over a ten-week period, participants wrote three draft essays, reviewed a peer’s unique draft using each application, and received peer feedback on their own drafts using each application. Comments were coded for the quantity, area, nature, location, and type of feedback. An entrance questionnaire elicited background information, and an exit questionnaire elicited quantitative and qualitative insights into the participants’ comparative experiences. The findings show that each application supports the exchange of peer feedback and that the applications’ feedback modes influence the reviewers’ comment foci. This study should help teachers make more informed decisions about which application is most appropriate for their desired peer feedback learning objectives.
Spoilt for choice
Authors: Satar H. Müge , Strobl Carola
Published: April 2018

Spoilt for choice: A plethora of modes for electronic feedback on second language writing
EFFECTS OF DEPTH AND CONCENTRATION OF DIGESTATE ON GROWTH OF THREE MICROALGAL SPECIES
Authors: Khanh Nguyen Tran Thien
Published: April 2018

In order to design a culture system for microalgal biomass production with a low cost and convenient cell collection, growth performance of mixtures of microalgal cells, including Euglena gracilis, Chlorella vulgaris, and Dunaliella tertiolecta cultured in a volume of 1 L were investigated at a PPFD of 300 µmol m-2 s-1 at the surface of the solution with continuous illumination at 30 °C. Each culture container contained diluted digestate at concentrations of 5, 10, 15, 20, and 50 %. Sample cells for counting cell number were collected daily at three depths: 0–50 mm (the surface layer), 10–15 mm (the middle layer), and 25–30 mm (the bottom layer). Pseudo-specific growth rates (ms) of each species at each depth were calculated as cellular multiplication rates using number of cells per time. In each layer, the average ms of each species was highest in 5 % digestate. The average ms of all three microalgal species (0.035 h-1) was observed in all layers in 5 % digestate solution. The ms of each species was highest in the bottom layer in 5% digestate (0.048 h-1, 0.041 h-1, and 0.022 h-1, respectively for C. vulgaris, E. gracilis, and D. tertiolecta). In conclusion, E. gracilis, C. vulgaris, and D. tertiolecta showed the highest specific growth rate in 5 % digestate in all layer.
Broadband GaAs pHemt LNA design for T/R module application
Authors: Le Phong Dai , Thong Vu Duy , Binh Pham Le
Published: April 2018

In this paper, a three stages monolithic low noise amplifier (LNA) for T/R module application is presented. This LNA is fully integrated on 0.15-um pHEMT GaAs technology and achieves a wide bandwidth from 6 GHz to 11 GHz. Within this band, the LNA has the minimum of 1.3 dB noise figure and over 25 dB small signal gain. The output third order interception point (OIP3) is over 30 dBm and the 1 dB compression point (P1 dB) is 16 dBm at the output.
Crystallization in Couette-Taylor Crystallizer: Effect of Taylor Vortices Flow on the Nucleation and Reconstruction of L-Glutamic Acid in Cooling Crystallization
Authors: Trinh Huyen Thi Thanh , Nguyen Tuan Anh , Phan Thao Thanh , Khuu Quang Chau , Le Nhan Thi Hong , Dang Giang Truong , Nguyen Tuyet Dinh
Published: April 2018

The effect of Taylor vortices flow of Couette-Taylor (CT) crystallizer on the nucleation and reconstruction of L-glutamic acid was firstly investigated in cooling crystallization. Generally, L-glutamic acid has two kinds of polymorphic crystal including metastable α-form and stable β-form, where the α-form is initially crystallized and then slowly transformed into the β-form, which is called the phase transformation. The present study explored that there was a significant difference between CT and the conventional ST crystallizer as regards the nucleation and reconstruction of L-glutamic acid. Here, the phase transformation determined by the nucleation and reconstruction was 40h in ST crystallizer, yet it was only 20h as using the CT crystallizer, implying that the nucleation and reconstruction of L-glutamic acid was facilitated 2.0 times as using the Taylor vortices flow. The advantage of Taylor vortices flow in CT crystallizer over the random fluid motion in ST crystallizer with regard to the nucleation and reconstruction was explained in terms of the high energy dissipation of Taylor vortices flow.
STUDY ON CHARACTERIZATION OF Ni/BIOCHAR CATALYST DERIVED FROM MICROALGAL BIOMASS
Authors: Pham Vuong Van , Nguyen Hong Khanh Dieu
Published: April 2018

Biochar supported nickel (Ni/biochar) catalyst was prepared by incipient wetness method and characterized by using a series of techniques such as XRD, SEM, TEM, FT-IR, H2-TPR and BET. These charaterizations indicated the catalyst structure and demonstrated its potential for applications in reduction – oxidation reactions in particular the HDO process.
STUDY ON IMPACT FORCES OF THE UNDERWATER CAVITY PROJECTILE
Authors: Dung Nguyen Thai , Thuyen Nguyen Duc
Published: April 2018

The motion of the underwater projectile with cavity effect including two motions: the projectile moves in the forward direction, center of mass of the projectile rotation around its nose makes tail of the projectile impacts on the cavity wall. According to, the impact forces occur, they include the drag force at its none, the impact force at impact point. The paper studies the forces occur on during motion of the underwater cavity projectile. Added, this paper considers the effect of the length and distributive projectile to the magnitude of impact force and the drag force of the underwater cavity projectile.
COMBINED MULTIVARIATE DATA ANALYSIS OF GC/MS AND SENSORY EVALUATION FOR AROMA QUALITY PREDICTION MODEL OF ORTHODOX BLACK TEA GRADES
Authors: Tuan Hoang Quoc , Thinh Nguyen Duy , Minh Tu Nguyen Thi
Published: April 2018

Relationships between sensory aroma and the volatile composition of 04 black tea grades produced from Northern Vietnam were studied. Consumer preference test on the aroma was carried out by 80 consumers to evaluate the aroma quality of these samples. Aroma concentrate was prepared by Brewed Extraction Method (BEM) method and analyzed using GC/MS. Partial Least Squares Regression (PLSR) was used to determine the relationship between preference scores and peak area percentage data of 39 detected volatile compounds. Among these compounds, 20 identified compounds were determined to contribute significantly to the perceived aroma quality of OTD black teas. On the basis of these 20 compounds, the PLSR model was constructed to predict the aroma quality of OTD black teas. The result showed that the volatile composition by GC/MS in the profiling with sensory and multivariate data analysis should be a useful reference for aroma quality prediction of OTD black tea grades.
FREE VIBRATION ANALYSIS OF LAMINATED STIFFENED CYLINDRICAL PANELS USING FINITE ELEMENT METHOD
Authors: Tuan Trinh Anh , Quoc Tran Huu , Tu Tran Minh
Published: April 2018

A study on the free vibration analysis of stiffened laminated composite cylindrical shell is described in this paper. The eight-noded isoparametric degenerated shell element is developed to model both shell panel and stiffeners by using the degenerated solid concept based on Reissner-Mindlin assumptions which taking to account the shear deformation and rotatory effect. Numerical results are presented and comparison is made with the published results from the literature and the good agreement is found. Parametric studies considering different geometrical variables of shell and stiffeners have also been carried out.
Evaluation of Average Directional Effective Emissivities of Isothermal Cylindrical-inner-cone Cavitties Using Monte-Carlo Method
Authors: Nguyen Quang Minh , Nguyen Van Binh
Published: April 2018

We have used the Monte Carlo method based on theory of successive reflections and ray tracing to calculate the average normal directional effective emissivities of isothermal cylindrical-inner-cone cavities for various geometrical parameters. A simplified specular-directional diffuse reflection model was applied in our calculations for cavities working in the infrared spectral range. Our results are in good agreement comparing with what obtained by other authors. The algorithm developed by us has an advantage in simplicity and time saving of calculations. It can be used in blackbody cavity design considerations, especially in the cylindrical-inner-cone cases.
Study of Mos Structures Using Nuclear Analytical Methods
Authors: Phuc Tran Van , Kulik M. , Kobzev A. P. , Khiem Le Hong
Published: April 2018

The atomic concentrations and depth distribution of elements in MOS (metal oxide semiconductor) structures have been investigated using two nuclear analytical methods: Rutherford Backscattering Spectrometry (RBS) and Elastic Recoil Detection (ERD). The elements with atomic masses in range from hydrogen up to copper were identified. Their depth profiles show that a MOS structure consists from metal (Al) layer, silicon oxide layer and a silicon substrate. The heavy elements Cu, Ti were found at near-surface area of one sample with low concentrations. The transitional area between the silicon substrate and the oxide layer as well as between the metal and oxide layers was noticed. The obtained results provide valuable information about MOS structures and concurrently demonstrate possibilities of both RBS and ERD methods in material analysis.
Depolarization Property of Cirrus Clouds Over Hanoi
Authors: Pham Minh Tien , Nguyen Xuan Tuan , Dinh Van Trung , Le Duy Manh , Bui Van Hai
Published: April 2018

Cirrus cloud is one of the most commonly occurring cloud types globally. The clouds are found mainly to consist of floating ice crystals, which can reflect strongly incoming radiation. Lidar measurements provide an opportunity to study the microphysics and ice compositions of cirrus clouds. We have developed a polarization lidar system to measure the depolarization characteristics of the lower atmosphere over Hanoi. From our measurements of cirrus clouds over the period from 2010 to 2012, the depolarization ratio of cirrus clouds is found to be very high ranging from 20% up to 80{\%}, indicating large fraction of backscattering from ice crystals. We find a trend of increasing depolarization ratio with height and decreasing temperature. We also compare our lidar measurements with other results obtained by lidar technique in other regions of the world.
Two Mode - (De)muxer Based on a Symmetric Y Junction Coupler, a \(2\times 2\) MMI Coupler and a Ridge Phase Shifter Using Silicon Waveguides for WDM Applications
Authors: Truong Dung Cao , Vu Dao Anh , Hoang Chung Vu
Published: April 2018

In this paper, we introduce a new two-mode (de)multiplexer based on the silicon-on-insulator (SOI) platform. The device is built on a symmetric Y-junction, a 2×2 multimode interference (MMI) waveguide and a phaseshifter in the form of a ridge waveguide which is designed using 3D scalar beam propagation method (BPM). The phase evolution in the structure is discussed in details. Simulation results show that the device can operate in a wide wavelength range (150 nm) with a low insertion loss and small crosstalk. Large fabrication tolerance to the width of the input waveguide up to 100 nm is achieved, which is compatible to the current CMOS manufacturing technologies for the photonic integrated circuits. Furthermore, the small footprint (4µm×286µm) makes the device suitable for applications in high bitrate and compact on-chip silicon photonic integrated circuits.
The role of aryl hydrocarbon receptor (AhR) in carcinogenesis in people with high level of dioxin
Authors: Dung Lê Thị Kim , Liên Nguyễn Thị Kim , Hoàng Nguyễn Huy , Hải Nông Văn
Published: April 2018

The Aryl hydrocarbon receptor (AhR) is a ligand activated transcription factor that is best known for mediating the toxicity and tumor promoting properties of the carcinogen, including of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD - dioxin). Numerous studies for harmful effects on human health of dioxin have been studied. The results showed that dioxin had effects on many of tissues and organs of nerve system, immune system, reproductive system leading to birth defects and causing development of cancers. The AhR is also now known to be involved in a variety of cellular processes, such as the cell cycle, epithelial barrier function, cell migration,and immune function. Through the AhR, dioxin influences the major stages of tumorigenesis- initiation, promotion, progression, and metastasis. Studies of aggressive tumors and tumor cell lines have revealed that AhR levels are elevated and can be found constitutively in the nucleus. A high degree of complexity has emerged regarding the role of AhR in cancer, with clear discrepancies between pro- and anti-tumorigenic activities when utilizing cell culture versus in vivo models of malignancy. Furthermore, various classes of AhR ligands and indeed ligands within the same class can differentially modulate AhR to influence tumorigenic outcomes. This leads to the hypothesis that the AhR is chronically activated in tumors, thus facilitating tumor progression. In this article, we review the results of studies in the literature about the role of AhR in carcinogenesis in humans with high level of dioxin. Information of the influence of AhR on the formation and development of tumors will be useful understanding to develop potential therapeutic modulation through AhR activities in tumors and to provide effective treatment for cancer patients.
Level of serum matrix metalloproteinase-9 in patients with acute coronary syndrome in Vietnam
Authors: Chí Đỗ Hữu , Phương Nguyễn Thị Minh , Huyền Bùi Thị , Dũng Nguyễn Tiến , Minh Phạm Đình , Lợi Đỗ Doãn , Huyền Nguyễn Thúy , Nhi Nguyễn Bích , Chi Phan Văn , Thảo Lê Thị Bích
Published: April 2018

Acute Coronary Syndrome (ACS) is one of the foremost causes of mortality and morbidity worldwide as well as Vietnam. ACS was evoked by rupture or erosion of atherosclerotic plaques that was divided into Non–ST-segment elevation Acute Coronary Syndrome (NSTEACS) and ST-segment elevation myocardial infarction groups (STEMI). The matrix metalloproteinase (MMPs) is an enzyme family with fucntioning in the degradation of extracellular matrix and disruption of basement membranes. Of the MMPs, MMP-9 is expressed in the atherosclerotic plaques and plays a key role in the rupture of vulnerable atherosclerotic plaques. In this work, we measured the serum MMP-9 level in 205 patients with acute coronary syndrome (including 103 of NSTE-ACS and 102 of STEMI patients) and 101 healthy participants by sandwich Elisa method. The results showed that the MMP-9 level was significantly higher in NSTE-ACS and STEMI compared to the control group (208.59 ± 100.47 ng/mL, 189.98 ± 112.81 ng/mL vs 168.50 ± 79.52 ng/mL, respectively, P=0.014). However, a logistic regression analysis indicated that the MMP-9 level was only significantly higher in patients with NSTE ACS (OR= 1.0048, CI 95%= 1.000-1.009; P= 0.018). The serum MMP-9 level is correlated with traditional risk factors such as glucose, Cholesterol and Triglyceride by multiple logistic regression analysis. Conclusion: Our study suggests that the serum MMP-9 level is significantly associated with the NSTEACS and is a potential marker for dianogsis of ACS in the Vietnamese patients
Study on creating the hybridoma for producing a monoclonal antibody that causes agglutination of the red blood cell B group
Authors: Phan Lê Văn , Trung Nguyễn Thị , Hải Trương Nam
Published: April 2018

ABO incompatibility is a potential lethal barrier in tranfusion therapy. ABO blood cell grouping for all of the donors and potential recipients is the unique way for ensuring the highest safety for potential recipients in blood transfusion. ABO blood cell grouping must be performed by both of the serum sample method and erythrocytes sample method. Nowadays, when applying the serum sample method, the monoclonal antibodies are commonly used to identify the antigens on the surface of the red blood cells. In this study, for the first time in Vietnam, the hybridoma technology was successefully developed and screened hybrid cells for producing monoclonal antibody specifically agglutinated with red blood cells B group. After being isolated from spleen and iliac lympho node of the human red blood cells B group immuned BALB/c mice, the lymphocytes B was fused with myeloma sp2/0. As the results, 10 single hybrid cell lines B4D6C6, B4D6E2, B4D10C9, B4D10B6, B4D10D5, B4D10D6, B4D10E4, B4H6C5, B8F6B6, B8F6D4 have been screened by a specific agglutination with sample red blood cells group B. Among them, the hybrid cell line B4D10C9 was the best secreting anti-B monoclonal antibody into culture, that presented by antibody titer reached 1/256, hence it was selected for further studies. The ELISA method helped to isotype the anti-B monoclonal antibody which was produced from B4D10C9 hybridoma. As a result, the anti-B monoclonal antibody contained IgM heavy chains and kappa light chains. The IgM antibody could be able to agglutinate red blood cells 25 times more than IgG antibody. The success of screening B4D10C9 hybridoma producing IgM monoclonal antibody is the most significant result of this study.
Genotypic, phylogenetic and epidemiological characteristics of porcine reproductive and respiratory syndrome virus strains (PRRSV) in Vietnam
Authors: Hòa Lê Thanh , Roan Đỗ Thị , Khuê Nguyễn Thị , Hương Đoàn Thị Thanh , Nga Nguyễn Thị Bích
Published: April 2018

Nucleotide sequence of ORF5 encoding the antigenic GP5 for 11 strains collected from different geographic localities in the country during 2008-2011 were obtained. These nucleotide sequences were analyzed for molecular properties (nucleotides and amino acids) to determine genotype, phylogenetic and molecular epidemiological characteristics compared with PRRSV circulating in Vietnam and worldwide. Analysis of nucleotides and deduced amino acids showed that there was very high level of nucleotide identity and amino acid homology (98 – 100%) between the Vietnamese and Chinese PRRSV strains. Phylogenetic analysis based on ORF5 nucleotide sequences revealed two large groups, one derived from the North American lineage of genotype 2, including 11 PRRSV isolates in this study and another of the European lineage of genotype 1. In the genotype 2, further subgroups were found among which there were strains of Asia (Vietnam, China, India), strains of the European/North American origin (Austria, US, Denmark) and a 2012-isolated strain from Canada, in a separate subgroup. Regarding to the epidemiological analysis, our isolates collected during 2008-2011, completely followed the endemic peaks occurred in Viet Nam, such as those in 2007-2008 and in 2010-2011. They all were determined as highly virulent strains due to high homology to the highly virulent strains of China occurred in those periods. We have some conclusions to be made that the outbreaks of highly pathogenic PRRSV in Vietnam were epidemiologically associated with the endemics occurred in China and originated from China.
Sequence analysis of thegene encoding polyhedrin of monodon baculovirus (MBV) infected in penaeid shrimp (Penaeus monodon) in Vietnam
Authors: An Nguyễn Thị Giang , Quyền Đồng Văn , Kháng Đinh Duy
Published: April 2018

Monodon Baculovirus (MBV) is a causative agent in farmed shrimp. It is a double-stranded DNA virus with genome size of 119.638 bp. MBV virions are free in the nuclei or covered by the occlusion bodies(Obs) constructed by polyhedrin matrix proteins of MBV. OBs serve to protect the occluded virions from the outside environment for extended periods until they are ingested by insect larvae. Polyhedrin is a type of protein covering the viruswith size from 17 to 23 nm, as well as a surface antigen used for Baculovirus detection. Sequence analysis of the gene coding for polyhedrin (polhed) showed that their open reading frame (ORF) was 1,371 bp long and coding for a polypeptide of 457 amino acids.The alignment of nucleotide sequencesof all polyhedrin genes including those of MBV isolated in Vietnam and from GenBank showed that polhed from Vietnam has 97%, 97% and 94% sequence identity compared to that of Thailand, Taiwan and India, respectively. Amino acid analysis revealed that the insertions occur at the sites 72-73 with 4 aa (p.72_73insNEPM) and at 81-82 with 7 aa (p.81_82ins PYHNSPN) in the MBV isolated in Vietnam in comparison with that of Thailand. In addition, we also observed other amino acid substitutions in the protein sequence as: p.Thr55Gly, p.Glu63Asp, p.Asn72Asp, p.Glu92Gly, p.Met93Leu, p.Lys108Asnand p.Lys113Asn. These amino acid changes may affect the protein structure and reduce the specificity of the antigen. Therefore, the foreign kits for MBV diagnosis may have less specificity when using for inland MBV strains detection. The result of our research suggests that MBV which cause diseases in Vietnamese shrimps have notable genetic deviation compared to that of Thailand’s and other countries’ in the world.
Effect of led light on in vitro growth and developmentof Anoectochilus roxburghii
Authors: Gấm Đỗ Thị , Chu Hoàng Hà Chu Hoàng Hà Chu Hoàng Hà , Phạm Bích Ngọc Phạm Bích Ngọc Phạm Bích Ngọc , Hưng Nguyễn Khắc , Khôi Phan Hồng , Bình Hà Thị Thanh , Chương Nguyễn Như , Nam Lường Tú , Bình Nguyễn Thị Thúy
Published: April 2018

Recently, LED lighting technology has been developed fast and strongly. It has been widely used in many new fields. LEDs have been tested as an artificial light source during micropagation and tissue culture of various plants species as an alternative to traditional light sources to save energy and improve the efficiency of the culture process. In this study, effects of LED light at different wavelengths and luminous intensities on growth of Anoectochilus roxburghii's buds in vitro were investigated. Anoectochilus's buds were cultured under the different light conditions, such as LED in monochromatic red (R), LED in monochromatic green (B) , LED in the green and red light combining with warm white light (W) in different ratios (BRW 1, BRW2, BRW3 and BR). After 3 months of culture, the results showed LED B at strong luminous intensity (79 ± 3 μmol.m-2.s-1) inhibited the growth and development of Anoectochilus roxburghii. In contrast, BR LED with low luminous intensity (30 ± 1μmol.m-2.s-1) had made a positive effect on growth and development of Anoectochilus roxburghii. The plant height (5,88 cm), root length (1,33 cm), fresh plant weight (0,169 g/plant), leaf area (0,82 cm2), fresh leaf weight (18,33 mg/leaves) of plants in vitro were found higher than those of plants grown under T5 fluorescent lighting conditions (as control). Besides, chlorophyll a, chlorophyll b and total chlorophyll content of leaves (285,40 µg/g, 196,40 µg/g, 481,80 µg/g respectively) were higher than in the control and other versions of LED combine. The results also showed that the LED light with combined ratio of BR = 1: 4 at luminous intensity of 30 μmol.m-2.s-1 were suitable for the growth of Anoectochilus roxburghii. Therefore, BR LED (1:4) light condition should be replaced fluorescent light sources in vitro culture of Anoectochilus roxburghii in future.
Biomass and lipid production of marine diatom Chaetoceros ChTA at different nutrient concentrations
Authors: Liên Nguyễn Thị Thu , Sơn Nguyễn Hồng , Nhân Lê Thị Tuyết
Published: April 2018

Microalgae, especially diatom, are an optimal resource of polyunsaturated fatty acids (PUFAs). Recently, the new industrial scale culture systems are being developed for the purpose of producing the large amounts of biomass for biofuel, medicinal, cosmetical production. However, an economic utilization system needs to be standardized for the optimal production of the resources. In this way, manipulation of culture conditions (physicochemical factors) represents a solution to increase the lipid yield. The strain Chaetoceros ChTA is one of the high potential lipid strains, isolated from Thuan An coastal zone, Thua Thien Hue province. This paper deals with the growth and total lipid content of the marine diatom strain, Chaetoceros ChTA at different concentrations of sodium nitrate, sodium phosphate and sodium silicate under the laboratory condition. In addition, the fatty acid composition of the strain Chaetoceros ChTA growing in f/2 medium (control formula) was also analyzed. The result showed that this diatom strain successfully growed with maximum biomass production of 0.482g/L at the 112.50 mg/L of sodium nitrate meanwhile maximum total lipid content of 25.62% dry cell weight was recorded in the medium amended with 2.13 mg/L sodium phosphate. The fatty acid composition of this strain in f/2 medium (control) was analysed through GC-MS and the result showed that the saturated fatty acid content was 32.3% while the content of unsaturated fatty acid was 65.9% of the total fatty acid (TFA). The fatty acid composition also contains a significant amount of EPA (14.33%) and DHA is suitable for navigation use them as functional foods, pharmaceuticals and feed supplements for animals in aquaculture.
Isolation, characterilzation and identification of phosphate solubilizing bacteria from rice-shrimp rotational farming system in salt affected soil areas of the mekong delta of Vietnam
Authors: Nghĩa Nguyễn Khởi , Oanh Nguyễn Thị Kiều
Published: April 2018

Phosphorus is the least mobile element in plant and soil contrary to other macronutrients. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants. Aim of study was to deal with isolation, characterization and identification of phosphate solubilizing bacteria (PSBs) from 15 paddy rice soil samples collected in rice–shrimp rotational farming system in salt affected soil areas of the Mekong Delta of Vietnam including Bac Lieu, Ca Mau, Soc Trang, Kien Giang and Ben Tre by using NBRIP media containing 1% NaCl. Concentration of phosphate was determined by method of colometric determination of molybdate. Results showed that 95 isolates indicating potentially as PSBs through a exhibiting of a halo zone happening around bacterial colonies on NBRIP media. Twenty out of ninety five isolates showed their highly phosphate solubilizing ability (>1000 mg.L-1) in the liquid culture. Bacterial strain coded as BL1-10 solubilized maximumly 2044 mg.L-1 phosphate in liquid media after 5 incubation days. The cell number and phosphate solubilizing ability of Bl-10 strain were till good and not affected under environmental coditions like 40oC, salinity range between 0.5 and 5 % NaC and pH range between 3 and 5. Three kinds of trade name fungicides like DA roral, Topsin M và Antracol 70WP did not affect on cell numbers and phosphate solubilizing ability of Bl-10 strain. However, cell numbers and function of phosphate solubilizing ability of this bacterial strain was completely inhibited by Ridomil Gold fungicide. This strain was genetically identified as species of Burkholderia sp. BL1-10 based on results of the 16S rRNA gene sequence analysis. In conclusion, the study suggests the most promising phosphate solubilizing bacteria, Burkholderia sp. BL1-10 can be used as efficient biofertilizer inoculant to promote plant growth.
Study on the transient expression of hemagglutinin antigen of influenza A/H7N9 virus in Nicotiana benthamiana using agro-infiltration
Authors: Thủy Lê Thị , Ngọc Lê Thu , Thương Hồ Thị , Ngọc Phạm Bích , Nhân Lâm Đại , Hà Chu Hoàng
Published: April 2018

Avian influenza A/H7N9 is a subtype of influenza viruses that has been detected in birds in the past. This

subtype has not been previously seen in either animals or human until it was found in March 2013 in China. However, since then, infections of modified H7N9 in both human and birds have been observed such as H7N2, H7N3 and H7N5 subtypes. Hemagglutinin (HA) is the most important antigen for induction of immunity, and is a target to develop influenza vaccines. In this study, the gene encoding HA protein was sucessfully inserted into pRTRA vectors to generate 35S-HA-histag-cmyc-ELP-KDEL and 35S-HApII-histag-cmyc-ELP-KDEL cassettes in the absence and presence of trimer motif GCN4, respectively in order to enhance accumulation of recombinant proteins in plants. These cassettes were inserted into pCB301 binary vectors and transformed into Agrobacterium tumefaciens in order to carry out the transient expression in Nicotiana benthamiana leaves. Five days after infiltration, the total soluble protein was extracted and analysed in SDS-PAGE. The HA-ELP and (HApII-ELP)3 recombinant proteins were detected by Western blot using anti-cmyc monoclonal antibody. These results showed that HA protein fused ELP was observed in monomeric and trimeric forms. The plant extract containing hemagglutinin protein in monomeric and trimeric forms were characterized bio-function using hemagglutination assay. The plant extract containing hemagglutinin protein in trimeric form agglutinated chicken red blood cells at concentration of 94 µg protein/ml whereas the plant extract containing hemagglutinin protein in monomeric form did not agglutinate chicken red blood cells at the same of protein concentration.
Preparation of the oligochitosan-silver nanoparticles with a potential application as an antigungal fertilizer against Fusarium oxysporum causing fusarium wilt disease on tomato
Authors: Vũ Nguyễn Thanh , Quyền Huỳnh , Xô Dương Hoa , Luân Lê Quang
Published: April 2018

Chitosan with molecular weight (Mw) approx. 573,2 kDa and deacetylation degree about 80% was irradiated by gamma rays at swollen state in the present of 1 - 3% hydrogen peroxide for degradation to prepare low Mw products. The solution of 5% low Mw chitosan in 0.5% acetic acid was further irradiated for induction of oligochitosan. The obtained results indicated that the foliar application by 100 ppm oligochitosan with Mw ~ 19.5 kDa prepared by 4kGy-irradiation of the low Mw chitosan with Mw ~ 48.4 kDa had a strongly growth promotion effect on tomato plants after 30 days cultivation. The oligochitosan silver nanoparticles (AgNPs) was also synthesized by gamma irradiation of solution contained 5% low Mw chitosan and 1 mM silver nitrate in order to prepared antifungal fertilizer for tomato plants. The results on in vitro test for the antifungal activity oligochitosan AgNPs against Fusarium oxysporum causing Fusarium wilt disease pointed out that the addition of this product into cultivated media inhibited strongly the growth of the Fusarium oxysporum pathogen fungus on carrot agar media. The antifungal efficiency values were found from 50.5 to 94.9% by the supplementions of silver concentrations from 20 to 30 ppm, respectively. The supplementation with a silver concentration of 40 ppm inhibited completely the growth of F. oxysporum on the cultivated media. Thus the oligochitosan AgNPs product had not only the growth promotion activity for tomato, but also high antifungal activity against F. oxysporum. This product is promising to be used as an antifungal fertilizer for tomato with safety and efficiency.
Transformation of glycosyltransferase dnrs and auxiliary DNRQ genes into the Streptomyces peucetius MH9.2 for the enhance of doxorubicin production
Authors: Thuy Ta Thi Thu , Hoang Nguyen Huy
Published: April 2018

Doxorubicin antibiotic produced by Streptomyces peucetius is a very crucial antibiotic with a large spectrum used as an anticancer drug and in clinical treatment. Production of doxorubicin from wild type strain by microbiological fermentation process is nomally very low yield and limited. The enhancement of doxorubicin production (DXR) by the genetically engineering of the auxiliary genes involved in the pathway of doxorubicin biosynthesis from Streptomyces peucetius has being focused by many research groups. The gene cluster responsible for biosynthesis of doxorubicin had been isolated, sequenced and characterized. Among them, the dnrQ and dnrS genes belong to the gene cluster encoded for the glycosyltransferase and helper enzymes, which are responsible for attachment of sugar moiety to -rhodomycinon intermediate and regulation of doxorubicin production, respectively. In this research, the dnrQ and dnrS genes were isolated and cloned in the p25.1 vector containing the strong promoter (ermE*) to generate the p25.1QS as a recombinant vector. The obtained plasmid was transferred into the host strain Streptomyces peucetius MH9.2 by the protoplast transformation method to generate the S. peucetius MH9.2SQ strain. Doxorubicin production from the recombinant S. peucetius MH9.2SQ and the parent strain were extracted and purified by using chlorofrom and methanol solvents. The products were confirmed by HPLC, LC-mass analysis and antibacterial bioassay. The results showed that the over production of doxorubicin was accumulated by the recombinant strain from the fermentation broth. In the S. peucetius MH9.2SQ, the doxorubicin productivity was 3.1 to 3.6 folds higher than in the parent strain. This recombinant strain could be a potential candidate for further research in enhancement and larger biomass scale of DXR production.
Biosynthesis of morin-3-O-rhamnopyranoside in a genetically engineered Escherichia coli
Authors: Thuần Nguyễn Huy , Trung Nguyễn Thành , Quyền Đồng Văn , Hằng Vũ Thị Thu , Sohng Jae Kyung
Published: April 2018

Flavonoids are significant secondary metabolites of vascular plants. They have been proven to possess numerous types of anti-bacterial, anti-tumor, anti-oxidant and anti-inflammatory bioactivities etc. Thereby, there are many flavonoids extracted and purified from plants and have been used for biological tests. Nevertheless, the traditional extraction methods require a large amount of initial sample, tedious and professional techniques and low yield of target compound. Until present, the advanced development of genetic engineerings and recombinant protein as platform allow synthesis of high amount of different types of flavoid in short time. Escherichia coli is one of the most popular host for whole-cell biotransformation of flavonoids and their derivatives due to its simple genetical, physiological system and fast growth. In this paper, we described the biosynthetic method of morin-3-O-rhamnopyranoside from a genetically engineered E. coli using morin as substrate. In particular, the E. coli harboring biosynthetic gene cluster of activated TDP-L-rhamnose and gene encoding for glycosyltransferase was used as host for biotransformation. Morin was fed into the culture broth of recombinant E. coli for rhamnosylation. The formation of morin-3-O-rhamnopyranoside was then quantitied and qualified using Thin Layer Chromatography (TLC), Reverse Phase High Performance Liquid Chromatography (RP-HPLC) and Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometric (LC-ESI-MS/MS) assays. The highest yield of product (56.5 µM) was achieved after 48h, 32 oC and pH = 7.2-8.0. This result showed that morin-3-O-rhamnopyranoside was succesfully synthesized in the genetically engineered E. coli. Furthermore, metabolic engineering of intra-cellular system for improving absorption of substrate as well as excretion of product or enhancement of glycosyltransferase activity may increase the final yield of biotransformation process.