Purification of Gamma Glytamyl Transferase and Study Some Biochemical Variables in Sera Patients with Hepatitis B and C in Kirkuk City
Authors: Ibrahim Hadi Salih  , Sayran S. Saleh  
Published: March 2021

Hepatitis B and C are the most frequent causes of chronic hepatitis diseases in the world. A cross sectionalstudy was carried out in Kirkuk city from 17th of November 2019 to 25th of February 2020 . The number ofhepatitis patients under study was 80 hepatitis patients, 44 with hepatitis B (14 acute and 30 chronic) and 36with hepatitis C (11 acute and 25 chronic) Their ages were between 20-70 years old. These patients admittedto Hepatology and Gastroenterology Center of Azadi Teaching Hospital and Kirkuk General Hospital andThe control group matched the patients group included 30 individuals who were apparently healthy who wereconducted in this study to determine the level of activity of the enzyme (GGT) as well as to determine thelevel of a number of biochemical variables (ALT, AST, ALP, TSB, CRP, AFP) in both healthy subjects andpatient group with acute and chronic viral hepatitis B and C . The results showed that there was significantincrease in the activity of the GGT enzyme in patients with P-value (p? 0.01) compared to control group. Thepresent study showed that there was significant increase in the levels of (ALT, AST.ALP, TSB) in the bloodserums of patients with acute hepatitis B and C compared to chronic and the healthy group. The present studyalso included the purification and partial isolation of the enzyme (GGT) and find the approximate molecularweight for it, that was purified from the blood serum of patients with hepatitis B and C and control group byusing ammonium sulfate, Dialysis and gel filtrationchromatography with sephadex (G-150) to separate theenzyme from other proteins. The degree of purification in type C was (31.2) times, type B (27) times, andwith healthy (30.2) times. The highsingle peak were used to determine the approximate molecular weight ofthe enzyme, and using the gel filtration technique. The approximate molecular weight of the enzyme (GGT)was up to (371,000-346,000-316,000) Dalton for healthy and hepatitis C and B patients, respectively. Inacute and chronic viral hepatitis B and C, there was a positive correlation between the efficacy of (GGT) andthe efficacy of GPT, CRP and AFP. The study showed that there was significant increase in the activity of theGGT enzyme in patients compared to control group. The results showed in Table 3 that there was significantincrease in the activity of the ALT enzyme in patients compared to control group. After performing theprocess of separating and purifying the GGT enzyme from the serum of patients with viral hepatitis B andC, using 40-60% of ammonium sulfate and after obtaining the required purity separating it from the rest ofthe components of the blood serum, the activity of the GGT enzyme was measured and it was found to haveoverall effectiveness in patients with hepatitis Liver C was (0.282) and the number of purification timeswas (1.63) times and the specific effectiveness was (1843). Hepatitis B was (3.5) times, and the results fromthe dialysis process of the precipitate resulting from the saline precipitation process showed an increase inthe specific activity of the enzyme GGT, so it was (3.4) for healthy people, (3.521) for patients with HCVand (4.3) HBsAg. Then he used gel filtration chromatography using the gel (Sephadex G-150), the resultsshowed that a single protein beam appeared, which is highly effective and has a degree of purificationreaching (30.2) And (31.2) and (27) times for GGT enzyme separated by gel filtration technique when theprotein solution obtained from the blood serum of healthy subjects and patients with viral hepatitis is passedfrom the separation column containing gel (G-150).