bluuebunny/crossref_metadata_2025 huggingface dataset:
Serum Albumin in Health and Disease: From Comparative Biochemistry to Translational Medicine
Authors: Belinskaia Daria A. , Jenkins Richard O. , Goncharov Nikolay V.
Published: September 2023

Being one of the main proteins in the human body and many animal species, albumin plays a decisive role in the transport of various ions, electrically neutral molecules and in maintaining the colloid osmotic pressure of the blood [...]
Humoral and Cellular Immune Response Elicited by the BNT162b2 COVID-19 Vaccine Booster in Elderly
Authors: Dalla Gasperina Daniela , Veronesi Giovanni , Castelletti Carlo M. , Varchetta Stefania , Ottolini Sabrina , Mele Dalila , Ferrari Giuseppe , Shaik Amruth K. B. , Celesti Fabrizio , Dentali Francesco , Accolla Roberto S. , Forlani Greta
Published: September 2023

Although the safety and efficacy of COVID-19 vaccines in older people are critical to their success, little is known about their immunogenicity among elderly residents of long-term care facilities (LTCFs). A single-center prospective cohort study was conducted: a total IgG antibody titer, neutralizing antibodies against Wild-type, Delta Plus, and Omicron BA.2 variants and T cell response, were measured eight months after the second dose of BNT162b2 vaccine (T0) and at least 15 days after the booster (T1). Forty-nine LTCF residents, with a median age of 84.8 ± 10.6 years, were enrolled. Previous COVID-19 infection was documented in 42.9% of the subjects one year before T0. At T1, the IgG titers increased up to 10-fold. This ratio was lower in the subjects with previous COVID-19 infection. At T1, IgG levels were similar in both groups. The neutralizing activity against Omicron BA.2 was significantly lower (65%) than that measured against Wild-type and Delta Plus (90%). A significant increase of T cell-specific immune response was observed after the booster. Frailty, older age, sex, cognitive impairment, and comorbidities did not affect antibody titers or T cell response. In the elderly sample analyzed, the BNT162b2 mRNA COVID-19 vaccine produced immunogenicity regardless of frailty.
Emerging Gene Therapy Approaches in the Management of Spinal Muscular Atrophy (SMA): An Overview of Clinical Trials and Patent Landscape
Authors: Ponomarev Aleksei S. , Chulpanova Daria S. , Yanygina Lina M. , Solovyeva Valeriya V. , Rizvanov Albert A.
Published: September 2023

Spinal muscular atrophy (SMA) is a rare autosomal recessive neuromuscular disease that is characterized by progressive muscle atrophy (degeneration), including skeletal muscles in charge of the ability to move. SMA is caused by defects in the SMN1 gene (Survival of Motor Neuron 1) which encodes a protein crucial for the survival and functionality of neuron cells called motor neurons. Decreased level of functioning SMN protein leads to progressive degeneration of alpha-motor neurons performing muscular motility. Over the past decade, many strategies directed for SMN-level-restoration emerged, such as gene replacement therapy (GRT), CRISPR/Cas9-based gene editing, usage of antisense oligonucleotides and small-molecule modulators, and all have been showing their perspectives in SMA therapy. In this review, modern SMA therapy strategies are described, making it a valuable resource for researchers, clinicians and everyone interested in the progress of therapy of this serious disorder.
Heterotypic Cell Culture from Mouse Bone Marrow under Simulated Microgravity: Lessons for Stromal Lineage Functions
Authors: Markina Elena , Tyrina Ekaterina , Ratushnyy Andrey , Andreeva Elena , Buravkova Ludmila
Published: September 2023

Muscle and skeleton structures are considered most susceptible to negative factors of spaceflights, namely microgravity. Three-dimensional clinorotation is a ground-based simulation of microgravity. It provides an opportunity to elucidate the effects of microgravity at the cellular level. The extracellular matrix (ECM) content, transcriptional profiles of genes encoding ECM and remodelling molecules, and secretory profiles were investigated in a heterotypic primary culture of bone marrow cells after 14 days of 3D clinorotation. Simulated microgravity negatively affected stromal lineage cells, responsible for bone tissue formation. This was evidenced by the reduced ECM volume and stromal cell numbers, including multipotent mesenchymal stromal cells (MSCs). ECM genes encoding proteins responsible for matrix stiffness and cell-ECM contacts were downregulated. In a heterotypic population of bone marrow cells, the upregulation of genes encoding ECM degrading molecules and the formation of a paracrine profile that can stimulate ECM degradation, may be mechanisms of osteodegenerative events that develop in real spaceflight.
Cloning of PmMYB6 in Pinus massoniana and an Analysis of Its Function
Authors: He Yuan , Hao Qingqing , Chen Peizhen , Qin Yiyun , Peng Manqing , Yao Sheng , He Xin , Yu Qiong , Agassin Romaric Hippolyte , Ji Kongshu
Published: September 2023

Phenylpropanoids are crucial for the growth and development of plants and their interaction with the environment. As key transcriptional regulators of plant growth and development, MYB-like transcription factors play a vital role in the biosynthesis of phenylpropanoid metabolites. In this study, we functionally characterized PmMYB6, a Pinus massoniana gene that encodes an R2R3-MYB transcription factor. It was confirmed by qPCR that PmMYB6 was highly expressed in the flowers, xylem, and phloem of P. massoniana. By overexpressing PmMYB6 in tobacco and poplar, we found that transgenic plants had enlarged xylem, increased content of lignin and flavonoids, and up-regulated expression of several enzyme genes of the phenylpropane metabolism pathway to different degrees. The above research results indicate that PmMYB6 is involved in the metabolic flux distribution of different branches of the phenylpropane metabolic pathway, and the results may provide clues for the regulation of metabolic fluxes between flavonoids and the lignin biosynthesis pathways of P. massoniana, as well as provide a basis for the molecular breeding of P. massoniana.
Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering
Authors: Fernandez-Lopez Laura , Roda Sergi , Robles-Martín Ana , Muñoz-Tafalla Rubén , Almendral David , Ferrer Manuel , Guallar Víctor
Published: September 2023

Lipases have valuable potential for industrial use, particularly those mostly active against water-insoluble substrates, such as triglycerides composed of long-carbon chain fatty acids. However, in most cases, engineered variants often need to be constructed to achieve optimal performance for such substrates. Protein engineering techniques have been reported as strategies for improving lipase characteristics by introducing specific mutations in the cap domain of esterases or in the lid domain of lipases or through lid domain swapping. Here, we improved the lipase activity of a lipase (WP_075743487.1, or LipMRD) retrieved from the Marine Metagenomics MarRef Database and assigned to the Actinoalloteichus genus. The improvement was achieved through site-directed mutagenesis and by substituting its lid domain (FRGTEITQIKDWLTDA) with that of Rhizopus delemar lipase (previously R. oryzae; UniProt accession number, I1BGQ3) (FRGTNSFRSAITDIVF). The results demonstrated that the redesigned mutants gain activity against bulkier triglycerides, such as glyceryl tridecanoate and tridodecanoate, olive oil, coconut oil, and palm oil. Residue W89 (LipMRD numbering) appears to be key to the increase in lipase activity, an increase that was also achieved with lid swapping. This study reinforces the importance of the lid domains and their amino acid compositions in determining the substrate specificity of lipases, but the generalization of the lid domain swapping between lipases or the introduction of specific mutations in the lid domain to improve lipase activity may require further investigation.
Preliminary Studies on the Predation of the Mite Blattisocius mali (Acari: Blattisociidae) on Various Life Stages of Spider Mite, Thrips and Fruit Fly
Authors: Michalska Katarzyna , Jena Manoj Kumar , Mrowińska Agnieszka , Nowakowski Piotr , Maciejewska Daria , Ziółkowska Klaudia , Studnicki Marcin , Wit Marcin
Published: September 2023

Research in recent years has shown that some species of predatory mites, considered to be typically associated with soil and litter, can also be found on plants. Such species include Blattisocius mali, which is an effective predator of acarid mites, nematodes and the eggs of moths and which can disperse by means of drosophilid fruit flies. Apart from soil and litter or storage, it has also been recorded on the bark of apple trees and the leaves of strawberries, thus suggesting its possible predation of/feeding on herbivorous mites and insects. Our goal was to examine whether B. mali could consume different development stages of two polyphagous herbivores, the two-spotted spider mite, Tetranychus urticae, and the western flower thrips, Frankliniella occidentalis, as well as the drosophilid fruit fly Drosophila hydei. In 24 h cage tests, single, starved B. mali females consumed all types of prey offered, i.e., the eggs, males and females of spider mites; the first-instar larvae and prepupae of thrips; and the eggs and first-instar larvae of fruit flies. The potential for B. mali to prey upon these insects and mites was confirmed. However, to estimate whether it can also effectively reduce their population, additional tests on the predator’s survival, fecundity and prey preference are needed.
The Morphological Diversity of Dragon Lacewing Larvae (Nevrorthidae, Neuroptera) Changed More over Geological Time Scales Than Anticipated
Authors: Mengel Laura , Linhart Simon , Haug Gideon T. , Weiterschan Thomas , Müller Patrick , Hoffeins Christel , Hoffeins Hans-Werner , Baranov Viktor , Haug Carolin , Haug Joachim T.
Published: September 2023

Nevrorthidae, the group of dragon lacewings, has often been considered a relic group. Today, dragon lacewings show a scattered distribution, with some species occurring in southern Europe, Japan, Australia, and one in China. The idea that this distribution is only a remnant of an originally larger distribution is further supported by fossils of the group preserved in ambers from the Baltic region (Eocene, ca. 35–40 MaBP) and Myanmar (Kachin amber, Cretaceous, ca. 100 MaBP). Larvae of the group are slender and elongated and live mostly in water. Yet, larvae are in fact very rare. So far, only slightly more than 30 larval specimens, counting all extant and fossil larvae, have been depicted in the literature. Here, we report numerous additional specimens, including extant larvae, but also fossil ones from Baltic and Kachin amber. Together with the already known ones, this sums up to over 100 specimens. We analysed quantitative aspects of the morphology of these larvae and compared them over time to identify changes in the diversity. Despite the enriched sample size, the data set is still unbalanced, with, for example, newly hatched larvae (several dozen specimens) only known from the Eocene. We expected little change in larval morphology over geological time, as indicated by earlier studies. However, on the contrary, we recognised morphologies present in fossils that are now extinct. This result is similar to those for other groups of lacewings which have a relic distribution today, as these have also suffered a loss in diversity in larval forms.
Time of Return to Work (RTW) May Not Correlate with Patient-Reported Outcomes Measurements (PROM) at Minimum One Year Post Arthroscopic Bankart Repair
Authors: Kosior Mateusz , Sibilska Aleksandra , Piwnik Marcin , Borowski Andrzej , Prusaczyk Szymon , Rogers Jason , Struzik Sławomir , Kwapisz Adam
Published: September 2023

It is widely recognized that work serves a dual role by not only ensuring financial independence but also functioning as a vital source of psychosocial well-being and contributing significantly to the attribution of meaning in life. The cost of work disability can be a multifactorial problem for both employers and workers; thus the inability to return to work (RTW) may have a destructive effect on mental health and confidence. Shoulder surgery is one of the conditions that inevitably impacts patients’ ability to work. As current data focus on restoring range of motion, strength, and the patients’ activity, to this day the data about RTW post shoulder surgery remain limited. The purpose of this study was to evaluate the return-to-work time of patients treated with an arthroscopic Bankart repair and to evaluate if patient-reported outcomes (PROM) correlate with the incapacity to work after an arthroscopic Bankart repair. We performed a retrospective review by conducting a questionnaire with patients more than 12 months after surgery and we identified 31 patients who met the criteria for the study and were able to contact 17 of them. In this paper we demonstrated that on average among groups working physically and at the office we may expect patients who underwent arthroscopic Bankart repair to return to work within 7 weeks from the surgery, with office workers tending to return significantly faster with an average of 2.5 weeks (p = 0.0239).
L-Carnitine and Male Fertility: Is Supplementation Beneficial?
Authors: Mateus Filipa G. , Moreira Silvia , Martins Ana D. , Oliveira Pedro F. , Alves Marco G. , Pereira Maria de Lourdes
Published: September 2023

L-Carnitine, a natural antioxidant found in mammals, plays a crucial role in the transport of long-chain fatty acids across the inner mitochondrial membrane. It is used as a nutritional supplement by professional athletes, improving performance and post-exercise recovery. Additionally, its therapeutic applications, including those in male infertility, have been investigated, as it may act as a defense mechanism against the excessive production of reactive oxygen species (ROS) in the testis, a process that can lead to sperm damage. This effect is achieved by enhancing the expression and activity of enzymes with antioxidant properties. Nevertheless, the mechanisms underlying the benefits of L-Carnitine remain unknown. This review aims to consolidate the current knowledge about the potential benefits of L-Carnitine and its role in male (in)fertility. Considering in vitro studies with Sertoli cells, pre-clinical studies, and investigations involving infertile men, a comprehensive understanding of the effects of L-Carnitine has been established. In vitro studies suggest that L-Carnitine has a direct influence on somatic Sertoli cells, improving the development of germ cells. Overall, evidence supports that L-Carnitine can positively impact male fertility, even at a relatively low dose of 2 g/day. This supplementation enhances sperm parameters, regulates hormone levels, reduces ROS levels, and subsequently improves fertility rates. However, further research is needed to elucidate the underlying mechanisms and establish optimal doses. In conclusion, the role of L-Carnitine in the field of male reproductive health is highlighted, with the potential to improve sperm quality and fertility.
The Correlation of Aortic Neck Angle and Length in Abdominal Aortic Aneurysm with Severe Neck Angulation for Prediction of Intraoperative Neck Complications and Postoperative Outcomes after Endovascular Aneurysm Repair
Authors: Chinsakchai Khamin , Sirivech Thana , Moll Frans L. , Tongsai Sasima , Hongku Kiattisak
Published: September 2023

Objectives: Endovascular aneurysm repair (EVAR) in a hostile neck has been associated with adverse outcomes. We aimed to determine the association of infrarenal aortic neck angle and length and establish an optimal cutoff value to predict intraoperative neck complications and postoperative outcomes. Methods: This was a retrospective review of patients with an intact infrarenal abdominal aortic aneurysm (AAA) with severe neck angulation (>60 degrees) who underwent EVAR from October 2010 to October 2018. Demographic data, aneurysm morphology, and operative details were collected. The ratio of neck angle and length was calculated as the optimal cutoff value of the aortic neck angle-length index. The patients were categorized into two distinct groups using latent profile analysis, a statistical technique employed to identify concealed subgroups within a larger population by examining a predetermined set of variables. Intraoperative neck complications, adjunct neck procedures, and early and late outcomes were compared. Results: 115 patients were included. Group 1 (G1) had 95 patients with an aortic neck angle-length index ≤ 4.8, and Group 2 (G2) had 20 patients with an aortic neck angle-length index > 4.8. Demographic data and aneurysm morphology were not significantly different between groups except for neck length (p < 0.001). G2 had more intraoperative neck complications than G1 (21.1% vs. 55%, p = 0.005). Adjunctive neck procedures were more common in G2 (18.9% vs. 60%, p < 0.001). The thirty-day mortality rate was not statistically different. G1 patients had a 5-year proximal neck re-intervention-free rate comparable to G2 patients (93.7% G1 vs. 87.5% G2, p = 0.785). The 5-year overall survival rate was not statistically different (59.9% G1 vs. 69.2% G2, p = 0.891). Conclusions: Patients with an aortic neck angle-length index > 4.8 are at greater risk of intraoperative neck complications and adjunctive neck procedures than patients with an aortic neck angle-length index ≤ 4.8. The 5-year proximal neck re-intervention-free rate and the 5-year survival rate were not statistically different. Based on our findings, this study suggests that the aortic neck angle-length index is a reliable predictor of intraoperative neck complications during EVAR in AAA with severe neck angulation.
Technological Advances in a Therapy of Primary Open-Angle Glaucoma: Insights into Current Nanotechnologies
Authors: Zembala Julita , Forma Alicja , Zembala Roksana , Januszewski Jacek , Zembala Patryk , Adamowicz Dominik , Teresiński Grzegorz , Buszewicz Grzegorz , Flieger Jolanta , Baj Jacek
Published: September 2023

Glaucoma is a leading cause of irreversible blindness and is characterized by increased intraocular pressure (IOP) and progressive optic nerve damage. The current therapeutic approaches for glaucoma management, such as eye drops and oral medications, face challenges including poor bioavailability, low patient compliance, and limited efficacy. In recent years, nanotechnology has emerged as a promising approach to overcome these limitations and revolutionize glaucoma treatment. In this narrative review, we present an overview of the novel nanotechnologies employed in the treatment of primary open-angle glaucoma. Various nanosystems, including liposomes, niosomes, nanoparticles, and other nanostructured carriers, have been developed to enhance the delivery and bioavailability of antiglaucoma drugs. They offer advantages such as a high drug loading capacity, sustained release, improved corneal permeability, and targeted drug delivery to the ocular tissues. The application of nanotechnologies in glaucoma treatment represents a transformative approach that addresses the limitations of conventional therapies. However, further research is needed to optimize the formulations, evaluate long-term safety, and implement these nanotechnologies into clinical practice. With continued advancements in nanotechnology, the future holds great potential for improving the management and outcomes of glaucoma, ultimately preserving vision and improving the lives of millions affected by this debilitating disease.
Perioperative Outcomes, Comorbidities, and Complications following Total Shoulder Arthroplasty in Wheelchair Users: A Retrospective Cohort Analysis of a Nationwide Database
Authors: Prabhu Kevin , Nasr Andrew J. , Kasitinon Donald , Cabrera Alison , Lin Yen-Sheng
Published: September 2023

Impaired shoulder function hinders the ability of wheelchair users to maintain independence. The current state of the literature delineates the risks and benefits of surgical techniques for the management of shoulder pathologies. To the best of our knowledge, there is no study that has investigated complications following total shoulder arthroplasty (TSA) in wheelchair users. Utilizing the PearlDiver Mariner national administrative database, 72,108 patients were identified who underwent TSA with a concurrent diagnosis of a rotator cuff tear. Two matched cohorts, one of wheelchair users and one of non-wheelchair users, were created. Due to limitations within PearlDiver, one-year outcomes, including comorbidity and complication rates and readmission statistics, were compared between the two cohorts. Each matched cohort of 869 patients underwent TSA with a concurrent diagnosis of a rotator cuff tear. The rate of readmission in wheelchair users was greater than in non-wheelchair users (24.05% vs. 9.55%, OR: 3.00, CI: 2.279, 3.946). Patients in the wheelchair cohort exhibited higher rates of complications and comorbidities (p < 0.001). Among the most likely to be readmitted after TSA were patients with osteoarthritis, pulmonary heart disease, rheumatoid arthritis, and hypertension (p < 0.05). Significant differences in surgical outcomes existed between wheelchair and non-wheelchair users in terms of preoperative comorbidities, postoperative complications, and readmission rates.
Vegan Diet in Dermatology: A Review
Authors: Lee Hannah , Sim Nathan , Fotouhi Audrey , Daveluy Steven
Published: September 2023

Diet is known to play a role in the development of skin disorders. While a vegan diet is frequently described as a risk factor for skin disease secondary to nutritional deficiency, this risk may be overestimated. This review aims to debunk myths and provide information on skin disorders and inflammatory skin conditions that have associations with specific nutritional deficiencies in the context of a vegan diet. A literature search was performed for each nutrient and inflammatory skin disease using the PubMed/MEDLINE database and public health website pages concerning a vegan diet. The literature has individual cases reporting skin disease due to deficiencies in vitamin B2 and vitamin A in patients following a vegan diet. The recommended daily amounts of nutrients and vitamins can be fulfilled on a vegan diet. Vegan diets also avoid food groups such as dairy and other animal-based products, which holds benefits in inflammatory skin diseases including acne, psoriasis, hidradenitis suppurativa, and atopic dermatitis. Overall, the risk of skin disease secondary to nutritional deficiency in patients following a vegan diet is very low and likely over-emphasized. A well-balanced and conscientiously planned vegan diet can adequately provide the necessary amounts of proteins, vitamins, and minerals to support skin health.
Aortic Valve Stenosis and Cancer: Problems of Management
Authors: Santangelo Gloria , Moscardelli Silvia , Barbieri Lucia , Faggiano Andrea , Carugo Stefano , Faggiano Pompilio
Published: September 2023

Aortic valve stenosis and malignancy frequently coexist and share the same risk factors as atherosclerotic disease. Data reporting the prognosis of patients with severe aortic stenosis and cancer are limited. Tailoring the correct and optimal care for cancer patients with severe aortic stenosis is complex. Cancer patients may be further disadvantaged by aortic stenosis if it interferes with their treatment by increasing the risk associated with oncologic surgery and compounding the risks associated with cardiotoxicity and heart failure (HF). Surgical valve replacement, transcatheter valve implantation, balloon valvuloplasty, and medical therapy are possible treatments for aortic valve stenosis, but when malignancy is present, the choice between these options must take into account the stage of cancer and associated treatment, expected outcome, and comorbidities. Physical examination and Doppler echocardiography are critical in the diagnosis and evaluation of aortic stenosis. The current review considers the available data on the association between aortic stenosis and cancer and the therapeutic options.
Mediating Effects of the COVID-19 Pandemic on the Associations between Physical Activity and Physical Fitness; Cross-Sectional Study among High School Adolescents
Authors: Sunda Mirela , Gilic Barbara , Vuletic Petra Rajkovic , Pavlinovic Vladimir , Zenic Natasa
Published: September 2023

The COVID-19 pandemic and the imposed social distancing measures caused negative changes in physical activity levels (PALs) and physical fitness (PF) among adolescents, but the potential mediating effect of the pandemic on the association between PAL and PF is unknown. This study aimed to evaluate gender-specific associations between objectively measured PAL and indices of PF among high school adolescents during the period of the COVID-19 pandemic. The participants were 150 adolescents (101 females) aged 14–18 years; their anthropometrics were evaluated, and they were tested on PF (cardiorespiratory fitness (CRF; beep test), power (broad jump), flexibility (sit-and-reach test), and abdominal strength (sit-ups)) and PAL (using a pedometer for 7 days) during the pandemic period. A T-test was calculated to determine differences between genders. Associations between variables were evaluated using Pearson correlations. Additionally, multivariate taxonomic classification was used to sort participants into homogenous groups (clusters) according to their PF, and then analysis of variance (ANOVA) was performed to differentiate them according to PAL. For the total sample, PAL was correlated with CRF only (R = 0.25, p < 0.05), while gender-stratified correlations showed that PAL was significantly associated with CRF among girls only (R = 0.29, p < 0.05), which was additionally confirmed with multivariate cluster analysis and subsequent ANOVA. No association between PAL and PF was found for boys. The relatively low association between PAL and PF is most likely related to the mediating effect of the change in life circumstances during the COVID-19 pandemic, and limited applicability of pedometers in evaluating high-intensity PAL. Further studies in other age groups and environments are warranted.
Address Obfuscation to Protect against Hardware Trojans in Network-on-Chips
Authors: Mountford Thomas , Dhavlle Abhijitt , Tevebaugh Andrew , Mansoor Naseef , Dinakarrao Sai Manoj Pudukotai , Ganguly Amlan
Published: September 2023

In modern computing, which relies on the interconnection of networks used in many/multi-core systems, any system can be critically subverted if the interconnection is compromised. This can be done in a multitude of ways, but the threat of a hardware Trojan (HT) being injected into a system is particularly prevalent due to the increase in third-party manufacturers for system-on-chip (SoC) designs. With a local injection of an HT in an SoC, an adversary can gain access to information about applications running on the system by revealing specific communications of the SoC, and the network-on-chip (NoC) as a whole. This heavily compromises the system and gives information to the attacker, which can lead to more tailored, compromising attacks. In this paper, we demonstrate an HT that exploits communication patterns inside an SoC to reveal applications that are running on an NoC with multi/many-core processors. This is performed by leaking packet counts, after which the attacker then uses machine learning techniques to identify applications running on processors, and the SoC as a whole. We also propose a LUT-based obfuscation technique to limit the information available to the hardware Trojan. Our results indicate that this obfuscation method can reduce the accuracy of this attack from 99% to <8% in multi/many-core systems.
Aptamers as Theranostics in Cardiovascular Diseases
Authors: Ramchandani Manish , Kumari Priyanka , Goyal Amit K.
Published: September 2023

Cardiovascular disease (particularly atherosclerosis) is a leading cause of death around the world, and there still exists a need for improved diagnostic techniques and treatments to improve patient outcomes as well as minimize the disease’s global burden. Aptamers are short, single-stranded DNA or RNA molecules that are accompanied by unique characteristics such as specificity, high binding affinity, ease of cellular internalization, and rapid tissue accumulation capabilities, offering great potential as theranostic agents in cardiovascular diseases with significantly improved sensitivity and accuracy. These theranostic agents provide a combination of therapy and diagnostics in which aptamers may diagnose and treat disease simultaneously. Therefore, this review article summarizes the role of aptamer-based probes for imaging and theranostics in cardiovascular disease. It also provides insight into current research and future treatment techniques that are very relevant for future clinical practice with the aim of improving the quality of life of cardiovascular disease patients.
Reconstruction of Gut Bacteria in Spodoptera frugiperda Infected by Beauveria bassiana Affects the Survival of Host Pest
Authors: Peng Yuejin , Wen Shaohai , Wang Guang , Zhang Xu , Di Teng , Du Guangzu , Chen Bin , Zhang Limin
Published: September 2023

Spodoptera frugiperda (Lepidoptera: Noctuidae) is a migratory agricultural pest that is devastating on a global scale. Beauveria bassiana is a filamentous entomopathogenic fungus that has a strong pathogenic effect on Lepidoptera pests but little is known about the microbial community in the host gut and the dominant populations in fungus-infected insects. B. bassiana AJS91881 was isolated and identified from the infected larvae of Spodoptera litura. The virulence of AJS91881 to the eggs, larvae, pupae and adults of S. frugiperda was measured. Moreover, the gut microbial community diversity of healthy and fungus-infected insects was analyzed. Our results showed that after treatment with B. bassiana AJS91881, the egg hatching rate, larval survival rate and adult lifespan of the insects were significantly reduced, and the pupae rigor rate was significantly increased compared to that of the control group. Additionally, the gut microbial community was reconstructed after B. bassiana infection. At the phylum and genus level, the relative abundance of the Proteobacteria and Serratia increased significantly in the B. bassiana treatment group. The KEGG function prediction results showed that fungal infection affected insect gut metabolism, environmental information processing, genetic information processing, organism systems and cellular processes. Fungal infection was closely related to the metabolism of various substances in the insect gut. Serratia marcescens was the bacterium with the highest relative abundance after infection by B. bassiana; intestinal bacteria S. marcescens inhibited the infection of insect fungi B. bassiana against the S. frugiperda. The presence of gut bacteria also significantly reduced the virulence of the fungi against the insects when compared to the group with the larvae fed antibiotics that were infected with fungal suspension (Germfree, GF) and healthy larvae that were infected with fungal suspension prepared with an antibiotic solution (+antibiotic). In conclusion, the reconstruction of the insect intestinal bacterial community is an indispensable link for understanding the pathogenicity of B. bassiana against S. frugiperda. Most importantly, in the later stage of fungal infection, the increased abundance of S. marcescens in the insect intestine inhibited the virulence of B. bassiana to some extent. The findings aid in understanding changes in the gut microbiota during the early stages of entomopathogenic fungal infection of insects and the involvement of insect gut microbes in host defense mediated by pathogenic fungal infection. This study is also conducive to understanding the interaction between entomopathogenic fungi, hosts and gut microbes, and provides a new idea for the joint use of entomopathogenic fungi and gut bacteria to control pests.
Unraveling the Impact of Lockdowns on E-commerce: An Empirical Analysis of Google Analytics Data during 2019–2022
Authors: Bâra Adela , Oprea Simona-Vasilica , Bucur Cristian , Tudorică Bogdan-George
Published: September 2023

This paper presents an empirical analysis of e-commerce data obtained through Google Analytics (GA) from two small businesses’ perspectives: an IT components company and a tourism agency website located within the same county in Romania. The objective of our study is to examine the enduring effects of the COVID-19 pandemic and seasonal variations over the last four years. The data collection spanned from January 2019, predating the onset of the COVID-19 pandemic, until mid-February 2023. To facilitate our analysis, we categorize the GA metrics into groups that encompassed website performance, site accessibility, and user behavior for the IT company. As for the tourism agency, we focus on website accessibility, user behavior, and marketing campaigns. Our goal is to empirically group or associate GA metrics according to their intrinsic meaning and check if each group reflects a certain latent concept (such as user behavior or site accessibility). Furthermore, our study aims to formulate and test five hypotheses regarding the immediate and long-lasting impact of the COVID-19 pandemic on the operations of small businesses. Our contribution consists of formulating and verifying the five hypotheses by providing descriptive data from the results of the Pearson correlation test, empirically grouping the GA metrics and verifying whether they reflect certain latent factors or topics, interpreting the results from the application of the ANOVA technique and Scarpello’s adaptation of the one factor test, respectively.
Deindustrialization, Tertiarization and Suburbanization in Central and Eastern Europe. Lessons Learned from Bucharest City, Romania
Authors: Săgeată Radu , Mitrică Bianca , Cercleux Andreea-Loreta , Grigorescu Ines , Hardi Tamás
Published: September 2023

This paper intends to delve deeply into the current understanding of the ways in which the transition from a central-based economy to an economy relying on free competition has led to changes in the big urban centers, bringing about a change in the relationships with the suburban areas. The authors take into account the high population density, the lack of space, and the elevated price of land within the big cities, which leads to urban functions migrating beyond the administrative boundaries, thus favoring the process of suburbanization. Given the context, commercial forces shift, migrating from the center to the urban peripheries or even outside them. This research is based on a comprehensive process of participative investigation (2012–2022) in Bucharest, Romania’s capital city. The research relies on field investigation, statistical and quantitative analyses and bibliographical sources. The conclusions rely primarily on the idea that political changes cannot be separated from economic, cultural and environmental ones, highlighting globalizing flows and the development of big cities. Industrial activities, strongly developed within a central-based economy, have significantly declined, which is partly compensated for by the development of the tertiary sector and, in particular, of commercial services leading to a functional reconversion of the urban peripheries and of suburban areas. The conclusions suggest that it is very important to be highly careful regarding the dilemmas and challenges ensuing from uncontrolled urban growth; therefore, several measures of urban planning should be taken with a view to achieving a better cooperation between urban stakeholders and those from the metropolitan areas so as to attain some common objectives in infrastructure in order to reach an integrated regional development.
Significance of Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) for the Monitoring of Treatment Response to Cyclosporine in Patients with Psoriasis
Authors: Frątczak Aleksandra , Miziołek Bartosz , Łupicka-Słowik Agnieszka , Sieńczyk Marcin , Polak Karina , Bergler-Czop Beata
Published: September 2023

Neutrophil gelatinase-associated lipocalin (NGAL) may promote development of inflammation in psoriasis, whereas proprotein convertase subtilisin/kexin type 9 (PCSK9) may account for dyslipidemia in some psoriatic patients. The aim of the study was to analyze the influence of cyclosporine therapy on serum levels of NGAL and PCSK9 in patients with psoriasis vulgaris. Methods: Serum samples were obtained before and after three months cyclosporine therapy. Patients were grouped into responders and non-responders to cyclosporine depending on whether they achieved at least 50% reduction of Psoriatic Activity Score Index (PASI), or not. Serum levels of PCSK9 and NGAL were assayed using commercially available ELISA tests. Lipid levels were measured with an enzymatic method. Results: There were 40 patients enrolled. A significant decrease in serum NGAL level was seen in cyclosporine responders. No similar dependance was found for PCSK9. Serum PCSK9 concentration correlated with total cholesterol (TChol) and LDL at baseline and after three month treatment. Conclusions: Cyclosporine therapy contributes to the reduction of the NGAL serum but not the PCSK9 concentration. Correlation between the PCSK9 serum level and TChol as well as LDL concentration may help to understand drug induced dyslipidemia after cyclosporine.
Influence of Cavitation on the Heat Transfer of High-Speed Mechanical Seal with Textured Side Wall
Authors: Yu Minfeng , Peng Xudong , Meng Xiangkai , Jiang Jinbo , Ma Yi
Published: September 2023

By setting textures on the side walls of a rotor, based on SST k-ω turbulence and the mixture model, the effects of depth-to-diameter ratio, shape, and rotational speed on interface temperature are analyzed. Local Nu number, flow field in textures, and gas distribution are used to verify the conclusion. When rotational speed increases, there are three different stages on the surface: liquid-dominated, mixed two-phase, and gas-dominated. This leads to a big difference in heat transfer on the side wall and causes the temperature on the seal face to increase when cavitation is considered. The distribution of the gas phase is explained through drag reduction, which has a high correlation with the velocity gradient near the surface. For several common shapes, heat transfer enhancement of textures is compared under high speed. The key influencing factor is the depth-to-diameter ratio, which causes flow stratification and reduces heat transfer. Flow stratification leads to different results of maximum temperature on the seal face when cavitation is considered. Results show that at high speed, a deep, circular texture is better when cavitation does not occur, and a shallow triangular texture is recommended when cavitation occurs; a textured side wall can reduce the maximum temperature of the seal face by about 10 °C.
Low-Cycle Fatigue Behavior of Wire and Arc Additively Manufactured Ti-6Al-4V Material
Authors: Springer Sebastian , Leitner Martin , Gruber Thomas , Oberwinkler Bernd , Lasnik Michael , Grün Florian
Published: September 2023

Additive manufacturing (AM) techniques, such as wire arc additive manufacturing (WAAM), offer unique advantages in producing large, complex structures with reduced lead time and material waste. However, their application in fatigue-critical applications requires a thorough understanding of the material properties and behavior. Due to the layered nature of the manufacturing process, WAAM structures have different microstructures and mechanical properties compared to their substrate counterparts. This study investigated the mechanical behavior and fatigue performance of Ti-6Al-4V fabricated using WAAM compared to the substrate material. Tensile and low-cycle fatigue (LCF) tests were conducted on both materials, and the microstructure was analyzed using optical microscopy and scanning electron microscopy (SEM). The results showed that the WAAM material has a coarser and more heterogeneous grain structure, an increased amount of defects, and lower ultimate tensile strength and smaller elongation at fracture. Furthermore, strain-controlled LCF tests revealed a lower fatigue strength of the WAAM material compared to the substrate, with crack initiation occurring at pores in the specimen rather than microstructural features. Experimental data were used to fit the Ramberg–Osgood model for cyclic deformation behavior and the Manson–Coffin–Basquin model for strain-life curves. The fitted models were subsequently used to compare the two material conditions with other AM processes. In general, the quasi-static properties of WAAM material were found to be lower than those of powder-based processes like selective laser melting or electron beam melting due to smaller cooling rates within the WAAM process. Finally, two simplified estimation models for the strain-life relationship were compared to the experimentally fitted Manson–Coffin–Basquin parameters. The results showed that the simple “universal material law” is applicable and can be used for a quick and simple estimation of the material behavior in cyclic loading conditions. Overall, this study highlights the importance of understanding the mechanical behavior and fatigue performance of WAAM structures compared to their substrate counterparts, as well as the need for further research to improve the understanding of the effects of WAAM process parameters on the mechanical properties and fatigue performance of the fabricated structures.
Influence of Low Temperature Plasma Oxidizing on the Bioactivity of NiTi Shape Memory Alloy for Medical Applications
Authors: Witkowska Justyna , Borowski Tomasz , Sowińska Agnieszka , Choińska Emilia , Moszczyńska Dorota , Morgiel Jerzy , Sobiecki Jerzy , Wierzchoń Tadeusz
Published: September 2023

The present study elucidates the impact of glow discharge oxidation within a low-temperature plasma environment on the bioactivity characteristics of an NiTi shape memory alloy. The properties of the produced surface layers, such as structure (TEM observations), surface morphology (SEM observations), chemical and phase composition (EDS and XRD measurements), wettability (optical gonimeter), and the biological response of osteoblasts and platelets to the oxidized surface compared with the NiTi alloy without a surface layer are presented. The presented surface modification of the NiTi shape memory alloy, achieved through oxidizing in a low-temperature plasma environment, led to the creation of a continuous surface layer composed of nanocrystalline titanium oxide TiO2 (rutile). The findings obtained from this study provide evidence that the oxidized layer augments the bioactivity of the shape memory alloy. This augmentation was substantiated through the spontaneous biomimetic deposition of apatite from a simulated body fluid (SBF) solution. Furthermore, the modified surface exhibited improved osteoblast proliferation, and enhanced platelet adhesion and activation. This proposed surface modification strategy holds promise as a prospective solution to enhance the biocompatibility and bioactivity of NiTi shape memory alloy intended for prolonged use in bone implant applications.
Microstructure and Mechanical Behavior of Quaternary Eutectic α+θ+Q+Si Clusters in As-Cast Al-Mg-Si-Cu Alloys
Authors: Li Kai , Yu Yan , Lu Qiang , Li Yuanfei , Yan Qiao , Lan Xinyue , Li Liya , Chen Baishan , Song Min
Published: September 2023

Cu additions notably strengthen Al-Mg-Si and Al-Si-Mg alloys due to the dense precipitation of quaternary nano precipitates during ageing. However, the chemical evolution and mechanical behaviors of the quaternary micro-scale Q constituent phase occurring in cast and homogenized states have rarely been studied. Meanwhile, there exists a type of AlCuMgSi cluster in the cast state, which has been regarded as Q particles. The accurate identification of phase constituents is the basis for the future design of alloys with better performance. In our work, this type of cluster was revealed to consist of α-Al, θ-Al2Cu, Q, and Si phases through micro-to-atomic scale studies using scanning and transmission electron microscopes. The skeleton of the dendrite was θ phase. The second phases in the dendritic eutectic cluster dissolved quickly during a 4 h homogenization at 550 °C. The Q phase was found to effectively absorb the Fe impurities during casting and homogenization. As a result, the formation of other harmful Fe-rich intermetallics was suppressed. These Q constituent particles were observed to break into separate pieces in an intermediately brittle manner when compressed in situ in a scanning electron microscope. These findings provide insights into the thermodynamic modeling of the Al-Mg-Si-Cu system and alloy design.
Removal of Silicon from Magnesite by Flotation: Influence of Particle Size and Mechanical Mechanism
Authors: Zhang Ruinan , Liu Zhaoyang , Yu Jingkun
Published: September 2023

The feasibility of producing high-density sintered magnesia with a one-step sintering method was investigated by utilizing finely ground magnesite as raw materials for direct flotation. The mechanism of flotation desilication of microfine grain magnesite was investigated by combining microstructure and chemical properties. The results showed that dodecylamine (DDA) has a sorting effect on magnesite reverse flotation desilication. Under the premise of 150 mg/L sodium polyacrylate (PAANa) as an inhibitor and 300 mg/L DDA as a collector, the content and recovery rate of MgO can reach 83.91% and 78.78%, respectively. When sodium oleate (NaOL) was used as a collector, the recovery rate of MgO was only 49.22%; therefore, it is unsuitable for magnesite purification. The flotation effect was affected because MgO particles and SiO2 particles agglomerated in the flotation process. The flotation agent cannot work for a single element but works for the mineral agglomerate. While collecting Si elements, the agglomerated MgO was also brought into the froth layer, making flotation impossible.
Asymptotic Behavior of Solutions to a Nonlinear Swelling Soil System with Time Delay and Variable Exponents
Authors: Kafini Mohammad M. , Al-Gharabli Mohammed M. , Al-Mahdi Adel M.
Published: September 2023

In this research work, we investigate the asymptotic behavior of a nonlinear swelling (also called expansive) soil system with a time delay and nonlinear damping of variable exponents. We should note here that swelling soils contain clay minerals that absorb water, which may lead to increases in pressure. In architectural and civil engineering, swelling soils are considered sources of problems and harm. The presence of the delay is used to create more realistic models since many processes depend on past history, and the delays are frequently added by sensors, actuators, and field networks that travel through feedback loops. The appearance of variable exponents in the delay and damping terms in this system allows for a more flexible and accurate modeling of this physical phenomenon. This can lead to more realistic and precise descriptions of the behavior of fluids in different media. In fact, with the advancements of science and technology, many physical and engineering models require more sophisticated mathematical tools to study and understand. The Lebesgue and Sobolev spaces with variable exponents proved to be efficient tools for studying such problems. By constructing a suitable Lyapunov functional, we establish exponential and polynomial decay results. We noticed that the energy decay of the system depends on the value of the variable exponent. These results improve on some existing results in the literature.