bluuebunny/crossref_metadata_2025 huggingface dataset:
Scalable Visible Light Indoor Positioning System Using RSS
Authors: Avendaño-Lopez Carlos M. , Castro-Sanchez Rogelio , Almanza-Ojeda Dora L. , Avina-Cervantes Juan Gabriel , Gomez-Martinez Miguel A. , Ibarra-Manzano Mario A.
Published: May 2022

This paper proposes a visible light positioning system that utilizes commercial Light-Emitting Diode (LED) lamps as transmitters and Silicon PIN photodiodes as receivers. The light signals are transmitted and received using Intensity Modulation and Direct Detection (IMDD). The lamps are modulated using On–Off Keying (OOK) with the Manchester code, and the medium access control is achieved by Time-Division Multiplexing (TDM). The position is estimated using trilateration based on the Received Signal Strength (RSS). The system’s scalability is accomplished by replicating primary localization cells composed of seven lamps and drawing on the neighborhood synchrony, exploiting the spatial multiplexing property of the light. A basic unit in the cell comprises three lamps forming a localization triangle; then, one primary localization cell shall consist of six triangles sharing lights among basic neighbor units. The cell prototype was implemented to prove the working principle of the system. Three estimation methods were used to compute the position: a deterministic approach based on least-squares regression, an Artificial Neural Network (ANN) per lamp, and an ANN for the complete system. The best per lamp estimator was the ANN, computing positions that reached an experimental accuracy of 2.5 cm under indoor conditions.
Software Release Assessment under Multiple Alternatives with Consideration of Debuggers’ Learning Rate and Imperfect Debugging Environment
Authors: Tian Qing , Fang Chih-Chiang , Yeh Chun-Wu
Published: May 2022

In the software development life cycle, the quality and reliability of software are critical to software developers. Poor quality and reliability not only cause the loss of customers and sales but also increase the operational risk due to unreliable codes. Therefore, software developers should try their best to reduce such potential software defects by undertaking a software testing project. However, to pursue perfect and faultless software is unrealistic since the budget, time, and testing resources are limited, and the software developers need to reach a compromise that balances software reliability and the testing cost. Using the model presented in this study, software developers can devise multiple alternatives for a software testing project, and each alternative has its distinct allocation of human resources. The best alternative can therefore be selected. Furthermore, the allocation incorporates debuggers’ learning and negligent factors, both of which influence the efficiency of software testing in practice. Accordingly, the study considers both human factors and the nature of errors during the debugging process to develop a software reliability growth model to estimate the related costs and the reliability indicator. Additionally, the issue of error classification is also extended by considering the impacts of errors on the system, and the expected time required to remove simple or complex errors can be estimated based on different truncated exponential distributions. Finally, numerical examples are presented and sensitivity analyses are performed to provide managerial insights and useful directions to inform software release strategies.
Analytic Expressions for Debye Functions and the Heat Capacity of a Solid
Authors: Gonzalez Ivan , Kondrashuk Igor , Moll Victor H. , Vega Alfredo
Published: May 2022

Analytic expressions for the N-dimensional Debye function are obtained by the method of brackets. The new expressions are suitable for the study of heat capacity of solids and the analysis of the asymptotic behavior of this function, both in the high and low temperature limits.
Back Analysis of Surrounding Rock Parameters in Pingdingshan Mine Based on BP Neural Network Integrated Mind Evolutionary Algorithm
Authors: Zhang Jianguo , Li Peitao , Yin Xin , Wang Sheng , Zhu Yuanguang
Published: May 2022

The mechanical parameters of surrounding rock are an essential basis for roadway excavation and support design. Aiming at the difficulty in obtaining the mechanical parameters of surrounding rock and large experimental errors, the optimized BP neural network model is proposed in this paper. The mind evolutionary algorithm can adequately search the optimal initial weights and thresholds, while the neural network has the advantage of strong nonlinear prediction ability. So, the optimized BP neural network model (MEA-BP model) takes advantage of the two models. It can not only avoid the local extreme value problem but also improve the accuracy and reliability of the prediction results. Based on the orthogonal test method and finite element analysis method, training samples and test samples are established. The nonlinear relationship between rock mechanical parameters and roadway deformation is established by the BP model and MEA-BP model, respectively. The importance analysis of the three input variables shows that the ∆D is the most important input variable, while ∆BC has the smallest impact. The comparison of prediction performance between the MEA-BP model and BP model demonstrates that the optimized initial weights and thresholds can improve the accuracy of prediction value. Finally, the MEA-BP model has been well applied to predicting the mechanical parameter for the surrounding rock in the Pingdingshan mine area, which proves the accuracy and reliability of the optimized model.
Epistemic Configurations and Holistic Meaning of Binomial Distribution
Authors: Fernández Coronado Nicolás Alonso , García-García Jaime I. , Arredondo Elizabeth H. , Araya Naveas Ismael Andrés
Published: May 2022

The competencies that today’s citizen must possess have led to a transformation of the teaching of probability, which has been repositioned on the school curriculum from an algorithmic view to a practical one based on the understanding of the concepts and their application in daily life. In this task, the understanding of the binomial distribution is essential as it allows the analysis of discrete data, the modeling of random situations, and the learning of other notions. However, weaknesses are identified in teachers and students with respect to the binomial distribution attributed to the lack of knowledge of its origin and meaning throughout history. For this reason, our work consists of the identification of its partial meanings and essential components as well as its relationships from a historical epistemological study and its analysis, based on the notions of the Ontosemiotic Approach (OSA) to Mathematical Knowledge and Instruction and the specialized literature on statistics and probability. As a result of our work, we present a reconstruction of the holistic meaning of the binomial distribution from the elements that compose it, which are essential for didactic purposes such as the identification and resolution of learning conflicts, the design or evaluation criteria, and teacher education.
Design of Aquila Optimization Heuristic for Identification of Control Autoregressive Systems
Authors: Mehmood Khizer , Chaudhary Naveed Ishtiaq , Khan Zeshan Aslam , Raja Muhammad Asif Zahoor , Cheema Khalid Mehmood , Milyani Ahmad H.
Published: May 2022

Swarm intelligence-based metaheuristic algorithms have attracted the attention of the research community and have been exploited for effectively solving different optimization problems of engineering, science, and technology. This paper considers the parameter estimation of the control autoregressive (CAR) model by applying a novel swarm intelligence-based optimization algorithm called the Aquila optimizer (AO). The parameter tuning of AO is performed statistically on different generations and population sizes. The performance of the AO is investigated statistically in various noise levels for the parameters with the best tuning. The robustness and reliability of the AO are carefully examined under various scenarios for CAR identification. The experimental results indicate that the AO is accurate, convergent, and robust for parameter estimation of CAR systems. The comparison of the AO heuristics with recent state of the art counterparts through nonparametric statistical tests established the efficacy of the proposed scheme for CAR estimation.
Array of Graphene Variable Capacitors on 100 mm Silicon Wafers for Vibration-Based Applications
Authors: Gikunda Millicent N. , Harerimana Ferdinand , Mangum James M. , Rahman Sumaya , Thompson Joshua P. , Harris Charles Thomas , Churchill Hugh O. H. , Thibado Paul M.
Published: May 2022

Highly flexible, electrically conductive freestanding graphene membranes hold great promise for vibration-based applications. This study focuses on their integration into mainstream semiconductor manufacturing methods. We designed a two-mask lithography process that creates an array of freestanding graphene-based variable capacitors on 100 mm silicon wafers. The first mask forms long trenches terminated by square wells featuring cone-shaped tips at their centers. The second mask fabricates metal traces from each tip to its contact pad along the trench and a second contact pad opposite the square well. A graphene membrane is then suspended over the square well to form a variable capacitor. The same capacitor structures were also built on 5 mm by 5 mm bare dies containing an integrated circuit underneath. We used atomic force microscopy, optical microscopy, and capacitance measurements in time to characterize the samples.
Sustainable Wax Coatings Made from Pine Needle Extraction Waste for Nanopaper Hydrophobization
Authors: Beluns Sergejs , Platnieks Oskars , Sevcenko Jekaterina , Jure Mara , Gaidukova Gerda , Grase Liga , Gaidukovs Sergejs
Published: May 2022

We combine renewable and waste materials to produce hydrophobic membranes in the present work. Cellulose nanopaper prepared from paper waste was used as a structural component for the membrane. The pine wax was reclaimed from pine needle extraction waste and can be regarded as a byproduct. The dip-coating and spray-coating methods were comprehensively compared. In addition, the solubility of wax in different solvents is reported, and the concentration impact on coating quality is presented as the change in the contact angle value. The sensile drop method was used for wetting measurements. Spray-coating yielded the highest contact angle with an average of 114°, while dip-coating reached an average value of 107°. Scanning electron microscopy (SEM) was used for an in-depth comparison of surface morphology. It was observed that coating methods yield significantly different microstructures on the surface of cellulose fibers. The wax is characterized by nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC). Pine wax has a melting temperature of around 80 °C and excellent thermal stability in oxygen, with a degradation peak above 290 °C. Fourier transform infrared spectroscopy (FTIR) was used to identify characteristic groups of components and show the changes on coated nanopaper. Overall, the results of this work yield important insight into wax-coated cellulose nanopapers and a comparison of spray- and dip-coating methods. The prepared materials have a potential application as membranes and packaging materials.
Oral Adelmidrol Administration Up-Regulates Palmitoylethanolamide Production in Mice Colon and Duodenum through a PPAR-γ Independent Action
Authors: Del Re Alessandro , Palenca Irene , Seguella Luisa , Pesce Marcella , Corpetti Chiara , Steardo Luca , Rurgo Sara , Sarnelli Giovanni , Esposito Giuseppe
Published: May 2022

Adelmidrol is a promising palmitoylethanolamide (PEA) analog which displayed up-and-coming anti-inflammatory properties in several inflammatory conditions. Recent studies demonstrated that Adelmidrol is an in vitro enhancer of PEA endogenous production, through the so called “entourage” effect. The present study investigated the ability of Adelmidrol (1 and 10 mg/Kg per os) to increase the endogenous level of PEA in the duodenum and colon of mice after 21-day oral administration in the presence and absence of PPAR-γ inhibitor (1 mg/kg). The level of PEA was analyzed by HPLC-MS. The expression of PEA-related enzymatic machinery was evaluated by western blot and RT-PCR analysis. Our findings demonstrated that Adelmidrol significantly increased PEA levels in the duodenum and colon in a dose/time-dependent manner. We also revealed that Adelmidrol up regulated the enzymatic machinery responsible for PEA metabolism and catabolism. Interestingly, the use of the selective irreversible PPAR-γ antagonist did not affect either PEA intestinal levels or expression/transcription of PEA metabolic enzymes following Adelmidrol administration. The “entourage effect” with Adelmidrol as an enhancer of PEA was thus PPAR-γ-independent. The findings suggest that Adelmidrol can maximize a PEA therapeutic-based approach in several intestinal morbidities.
Distinct Elevational Patterns and Their Linkages of Soil Bacteria and Plant Community in An Alpine Meadow of the Qinghai–Tibetan Plateau
Authors: Cong Jing , Cong Wei , Lu Hui , Zhang Yuguang
Published: May 2022

Soil microbes play important roles in determining plant community composition and terrestrial ecosystem functions, as well as the direction and extent of terrestrial ecosystem feedback to environmental changes. Understanding the distribution patterns of plant and soil microbiota along elevation gradients is necessary to shed light on important ecosystem functions. In this study, soil bacteria along an elevation gradient in an alpine meadow ecosystem of the Qinghai–Tibetan Plateau were investigated using Illumina sequencing and GeoChip technologies. The community structure of the soil bacteria and plants presented a continuous trend along the elevation gradient, and their alpha diversity displayed different distribution patterns; however, there were no linkages between them. Beta diversity of the soil bacteria and plants was significantly influenced by elevational distance changes (p < 0.05). Functional gene categories involved in nitrogen and phosphorus cycling had faster changes than those involved in carbon degradation, and functional genes involved in labile carbon degradation also had faster variations than those involved in recalcitrant carbon degradation with elevational changes. According to Pearson’s correlation, partial Mantel test analysis, and canonical correspondence analysis, soil pH and mean annual precipitation were important environmental variables in influencing soil bacterial diversity. Soil bacterial diversity and plant diversity had different distribution patterns along the elevation gradient.
SARS CoV-2-Induced Viral Sepsis: The Role of Gut Barrier Dysfunction
Authors: Assimakopoulos Stelios F. , Eleftheriotis Gerasimos , Lagadinou Maria , Karamouzos Vassilios , Dousdampanis Periklis , Siakallis Georgios , Marangos Markos
Published: May 2022

A considerable proportion of patients with severe COVID-19 meet Sepsis-3 criteria and share common pathophysiological mechanisms of multiorgan injury with bacterial sepsis, in absence of secondary bacterial infections, a process characterized as “viral sepsis”. The intestinal barrier exerts a central role in the pathophysiological sequence of events that lead from SARS-CoV-2 infection to severe systemic complications. Accumulating evidence suggests that SARS-CoV-2 disrupts the integrity of the biological, mechanical and immunological gut barrier. Specifically, microbiota diversity and beneficial bacteria population are reduced, concurrently with overgrowth of pathogenic bacteria (dysbiosis). Enterocytes’ tight junctions (TJs) are disrupted, and the apoptotic death of intestinal epithelial cells is increased leading to increased gut permeability. In addition, mucosal CD4(+) and CD8(+) T cells, Th17 cells, neutrophils, dendritic cells and macrophages are activated, and T-regulatory cells are decreased, thus promoting an overactivated immune response, which further injures the intestinal epithelium. This dysfunctional gut barrier in SARS-CoV-2 infection permits the escape of luminal bacteria, fungi and endotoxin to normally sterile extraintestinal sites and the systemic circulation. Pre-existing gut barrier dysfunction and endotoxemia in patients with comorbidities including cardiovascular disease, obesity, diabetes and immunosuppression predisposes to aggravated endotoxemia. Bacterial and endotoxin translocation promote the systemic inflammation and immune activation, which characterize the SARS-CoV-2 induced “viral sepsis” syndrome associated with multisystemic complications of severe COVID-19.
The Gut Microbiota May Affect Personality in Mongolian Gerbils
Authors: Gan Lin , Bo Tingbei , Liu Wei , Wang Dehua
Published: May 2022

The “gut–microbiota–brain axis” reveals that gut microbiota plays a critical role in the orchestrating behavior of the host. However, the correlation between the host personalities and the gut microbiota is still rarely known. To investigate whether the gut microbiota of Mongolian gerbils (Meriones unguiculatus) differs between bold and shy personalities, we compared the gut microbiota of bold and shy gerbils, and then we transplanted the gut microbiota of bold and shy gerbils into middle group gerbils (individuals with less bold and shy personalities). We found a significant overall correlation between host boldness and gut microbiota. Even though there were no significant differences in alpha diversity and beta diversity of gut microbiota between bold and shy gerbils, the Firmicutes/Bacteroidetes phyla and Odoribacter and Blautia genus were higher in bold gerbils, and Escherichia_shigella genus was lower. Furthermore, the fecal microbiota transplantation showed that changes in gut microbiota could not evidently cause the increase or decrease in the gerbil’s boldness score, but it increased the part of boldness behaviors by gavaging the “bold fecal microbiota”. Overall, these data demonstrated that gut microbiota were significantly correlated with the personalities of the hosts, and alteration of microbiota could alter host boldness to a certain extent.
Azospirillum spp. from Plant Growth-Promoting Bacteria to Their Use in Bioremediation
Authors: Cruz-Hernández María Antonia , Mendoza-Herrera Alberto , Bocanegra-García Virgilio , Rivera Gildardo
Published: May 2022

Xenobiotic contamination, a worldwide environmental concern, poses risks for humans, animals, microbe health, and agriculture. Hydrocarbons and heavy metals top the list of toxins that represent a risk to nature. This review deals with the study of Azospirillum sp., widely reported as plant growth-promoting bacteria in various cultures. However, its adaptation properties in adverse environments make it a good candidate for studying remediation processes in environments polluted with hydrocarbons and heavy metals. This review includes studies that address its properties as a plant growth promoter, its genomics, and that evaluate its potential use in the remediation of hydrocarbons and heavy metals.
Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation
Authors: Härer Luca , Hilgarth Maik , Ehrmann Matthias A.
Published: May 2022

It is known that the bacterial microbiota in beehives is essential for keeping bees healthy. Acetic acid bacteria of the genus Bombella colonize several niches in beehives and are associated with larvae protection against microbial pathogens. We have analyzed the genomes of 22 Bombella strains of different species isolated in eight different countries for taxonomic affiliation, central metabolism, prophages, bacteriocins and tetracycline resistance to further elucidate the symbiotic lifestyle and to identify typical traits of acetic acid bacteria. The genomes can be assigned to four different species. Three genomes show ANIb values and DDH values below species demarcation values to any validly described species, which identifies them as two potentially new species. All Bombella spp. lack genes in the Embden–Meyerhof–Parnas pathway and the tricarboxylic acid cycle, indicating a focus of intracellular carbohydrate metabolism on the pentose phosphate pathway or the Entner–Doudoroff pathway for which all genes were identified within the genomes. Five membrane-bound dehydrogenases were identified that catalyze oxidative fermentation reactions in the periplasm, yielding oxidative energy. Several complete prophages, but no bacteriocins, were identified. Resistance to tetracycline, used to prevent bacterial infections in beehives, was only found in Bombella apis MRM1T. Bombella strains exhibit increased osmotolerance in high glucose concentrations compared to Gluconobacter oxydans, indicating adaption to high sugar environments such as beehives.
Coculture of Trichoderma harzianum and Bacillus velezensis Based on Metabolic Cross-Feeding Modulates Lipopeptide Production
Authors: Fifani Barbara , Steels Sebastien , Helmus Catherine , Delacuvellerie Alice , Deracinois Barbara , Phalip Vincent , Delvigne Frank , Jacques Philippe
Published: May 2022

Cocultures have been widely explored for their use in deciphering microbial interaction and its impact on the metabolisms of the interacting microorganisms. In this work, we investigate, in different liquid coculture conditions, the compatibility of two microorganisms with the potential for the biocontrol of plant diseases: the fungus Trichoderma harzianum IHEM5437 and the bacterium Bacillus velezensis GA1 (a strong antifungal lipopeptide producing strain). While the Bacillus overgrew the Trichoderma in a rich medium due to its antifungal lipopeptide production, a drastically different trend was observed in a medium in which a nitrogen nutritional dependency was imposed. Indeed, in this minimum medium containing nitrate as the sole nitrogen source, cooperation between the bacterium and the fungus was established. This is reflected by the growth of both species as well as the inhibition of the expression of Bacillus genes encoding lipopeptide synthetases. Interestingly, the growth of the bacterium in the minimum medium was enabled by the amendment of the culture by the fungal supernatant, which, in this case, ensures a high production yield of lipopeptides. These results highlight, for the first time, that Trichoderma harzianum and Bacillus velezensis are able, in specific environmental conditions, to adapt their metabolisms in order to grow together.
Effect of Input C/N Ratio on Bacterial Community of Water Biofloc and Shrimp Gut in a Commercial Zero-Exchange System with Intensive Production of Penaeus vannamei
Authors: Xu Wujie , Wen Guoliang , Su Haochang , Xu Yu , Hu Xiaojuan , Cao Yucheng
Published: May 2022

Although increasing attention has been attracted to the study and application of biofloc technology (BFT) in aquaculture, few details have been reported about the bacterial community of biofloc and its manipulation strategy for commercial shrimp production. An 8-week trial was conducted to investigate the effects of three input C/N ratios (8:1, 12:1 and 16:1) on the bacterial community of water biofloc and shrimp gut in a commercial BFT tank system with intensive aquaculture of P. vannamei. Each C/N ratio group had three randomly assigned replicate tanks (culture water volume of 30 m3), and each tank was stocked with juvenile shrimp at a density of 300 shrimp m−3. The tank systems were operated with zero-water exchange, pH maintenance and biofloc control. During the trial, the microbial biomass and bacterial density of water biofloc showed similar variation trends, with no significant difference under respective biofloc control measures for the three C/N ratio groups. Significant changes were found in the alpha diversity, composition and relative abundance of bacterial communities across the stages of the trial, and they showed differences in water biofloc and shrimp gut among the three C/N ratio groups. Meanwhile, high similarity could be found in the composition of the bacterial community between water biofloc and shrimp gut. Additionally, nitrogen dynamics in culture water showed some differences while shrimp performance showed no significant difference among the three C/N ratio groups. Together, these results confirm that the manipulation of input C/N ratio could affect the bacterial community of both water biofloc and shrimp gut in the environment of a commercial BFT system with intensive production of P. vannamei. Moreover, there should be different operations for the nitrogen dynamics and biofloc management during shrimp production process under different C/N ratios.
Fluid Inclusion and Chemical Composition Characteristics of Emeralds from Rajasthan Area, India
Authors: Qin Li-Jie , Yu Xiao-Yan , Guo Hong-Shu
Published: May 2022

Emerald is among the most valuable gems in the world. Over the past decade, its commercial value and geographic origin have been the focus of gemological and geological research. In this study, emerald samples from India were examined by UV-Vis-NIR, FTIR, Raman spectra analysis, EPMA, and LA-ICP-MS. Hexagonal three- and multi-phase inclusions are first reported in Indian emeralds, containing gas bubbles (CO2 or CO2 + CH4), water or liquid mixtures of H2O + CO2, and solid phases inclusions (rounded crystals of siderite and dolomite, platelets of phlogopite, and magnesite). Mineral inclusions in Indian emeralds typically included phlogopite, quartz, talc, aragonite, and albite. The representative UV-Vis-NIR spectra show a distinct Fe absorption band, and one of the more typical characteristics of Indian emeralds is that the absorption strength of Fe3+ (369 nm) and Fe2+ (851 nm) is greater than that of Cr3+ (426, 606, 635, and 680 nm). Infrared spectra show that the absorption of type II H2O is stronger than that of type I H2O. LA-ICP-MS results show that Indian emeralds contain high alkali metals (10,503–16,964 ppmw; avg. 13,942 ppmw), moderate Fe (2451–4153 ppmw; avg. 3468 ppmw), low V (37–122 ppmw; avg. 90 ppmw), and the content of Cr (106–6310 ppmw) varies in a wide range. From a greenish-white core to a medium-green rim, the content of Fe, V, Cr, Sc, Cs, Rb, and Ga gradually increases in emerald with color band.
Temperature Variation Characteristics and Model Optimization of Flocculation Sedimentation of Overflow Ultra-Fine Iron Tailings
Authors: Niu Fusheng , Zhang Hongmei , Zhang Jinxia , Yu Xiaodong
Published: May 2022

In order to study the effect of temperature on the settling characteristics of overflow ultra-fine iron tailings, the settling velocity of overflow ultra-fine iron tailings at eight different temperatures at 10–80 °C was experimentally studied. The results show that, with the increase in slurry temperature, the flocculation settling velocity of overflow ultra-fine iron tailings increases first and then decreases. That is, when the temperature is less than 60 °C, the settling velocity of flocs increases with the increase in temperature. When the temperature is 60 °C, the settling velocity reaches the maximum 5.66 mm/s. When the temperature is more than 60 °C, the settling velocity of tailings flocs gradually decreases. In addition, with the increase in the test temperature, when the temperature is less than 60 °C, the particle size, fractal dimension, and density of tailings flocculant gradually increase, the gap of flocculant structure gradually decreases, and the floc structure becomes denser. When the temperature is higher than 60 °C, the particle size, fractal dimension, and density of flocs gradually decrease, and the gap between flocs is larger than that at 60 °C. On this basis, the temperature model of overflow ultra-fine iron tailings is established according to the analysis of particle settling process, and the settling model was optimized according to different settling areas. The mean absolute error between the optimized settling velocity and the actual velocity is 0.007, the root mean square error is 0.002, and the error is small. The theoretical calculation results are in good agreement with the experimental data, and the optimized flocculation settling model has an important role in promoting the theoretical study of the flocculation settling of such ultra-fine iron mineral particles, and can be used to guide the sedimentation and separation system to achieve good sedimentation treatment effect under the best working conditions as required.
Mineral Paragenesis Precipitating in Salt Flat Pools of Continental Environments Replicated in Microbial Mat Microcosms without Evaporation
Authors: Cabestrero Óscar , Tebes-Cayo Cinthya , Hinman Nancy W. , Demergasso Cecilia
Published: May 2022

Mineral precipitation can be observed in natural environments, such as lagoons, rivers, springs, and soils. The primary precipitation process has long been believed to be abiotic due to evaporation, leading to phase supersaturation. However, biotic interactions of microbial metabolism, organic compounds, and dissolved ions leading to mineral precipitation has been shown in laboratory studies using single-organism culture. The increase in pH inducing calcium carbonate precipitation due to oxygenic photosynthesis by Cyanobacteria and the release of ions due to organic matter decomposition by Firmicutes-inducing magnesium carbonate precipitation are recognized examples. As microbes do not live as pure cultures in natural environments but form complex communities, such pure culture lab studies do not reflect natural conditions. In this study, we grew natural complex microbial communities in microcosm conditions using filtered brine as water column and two types of natural gypsum substrates, and we replenished incubations to avoid evaporation. We monitored microbial communities through optical microscopy and analyzed mineral paragenesis in association with and without microbes, using different analytical techniques, such X-ray diffraction, and optical and field emission scanning electron microscopies. To detect changes throughout the experiment, small amounts of water column brine were extracted for physicochemial determinations. We were able to detect mineral paragenesis, avoiding evaporation, including major phases of chemical sedimentary rocks, such as gypsum, calcium carbonate, and some silicates in association to microbes. In addition, we evidenced that the use of natural substrates positively impacts growth of microbial communities, promoting the development of more biomass. This study can be seen as the first attempt and proof of concept of differentiating biotic and abiotic participation in evaporitic deposits, as they can form mineral paragenesis without evaporation. Future studies with microcosm experiments using microbial mats will be needed to establish mineral precipitation induced by micro-organisms and their extracellular polymeric substances (EPS), specifically to replicate mineral paragenesis sedimented from natural brines.
Microwave Dewatering of Gibbsite-Type Bauxite Ores: Permittivities, Heating Behavior and Strength Indices
Authors: Pickles Christopher , Lu Ting
Published: May 2022

Microwave radiation is a relatively new energy source that is being considered for several applications in mineral processing and extractive metallurgy. In the present research, various gibbsite-type bauxite ores were subjected to microwave radiation. The main objective was to assess the effect of microwave dewatering on the compressive strength indices of the ores and to compare the results obtained to those for conventional heating. Firstly, the fundamental interactions of the microwaves with the ores were evaluated by determining both the real and the imaginary permittivities as a function of temperature, and these were related to the water content. Secondly, the microwave heating behavior was modeled using a 24 factorial statistical analysis. Thirdly, the effect of dewatering by microwave heating on the compressive strength indices of roughly spherical bauxite ore pisoids was studied, and these results were compared to those obtained using conventional heating. Fourthly, the effect of particle size on the compressive strengths of irregular-shaped single particles of bauxite ore was investigated using both heating techniques. Finally, the energy requirements for dewatering of the ores, and hence reducing their compressive strengths, were compared for both processes. On the laboratory scale, the results showed that in comparison to conventional dewatering, microwave dewatering resulted in lower strength indices at both lower moisture removals and energy inputs.
Using Process Mineralogy as a Tool to Investigate Blending Potential of the Pentlandite-Bearing Ores at the Nkomati Ni Mine in South Africa
Authors: Dzvinamurungu Thomas , Rose Derek Hugh , Chimwani Ngonidzashe , Viljoen Fanus
Published: May 2022

The mineralogy and texture of Ni-sulfide ores at the Nkomati nickel mine are highly variable, and this results in often erratic nickel recovery at the mine. The variability of the ore presents an opportunity to study the influence of grind size on the flotation-based recovery of Ni in highly heterogeneous sulfide ores, which would be applicable to this ore type at many other mines worldwide. In view of this, a process mineralogy investigation was conducted on thirteen mineralogically and texturally different nickel-sulfide ores from the Nkomati Nickel Mine, with a view on the influence of grind size on the flotation performance of pentlandite. Ore types presented include medium- and high-grade variants of the bleb, disseminated, massive, semi-massive, and net-textured sulfide ores of the Main Mineralized Zone (MMZ), as well as disseminated chromite-rich nickel sulfide ore and massive chromitite ore of the Peridotitic Chromitite Mineralized Zone (PCMZ). Laboratory scale metallurgical test work, comprising of sequential grinding and bench-top flotation testing of the ores, was conducted in combination with quantitative mineralogical investigation of the flotation feed and associated flotation products, using a FEI 600F Mineral Liberation Analyzer. The ore types under consideration require a variety of grind sizes (i.e., milling times) in order to attain optimal recovery of nickel through flotation. This is predominantly controlled by ore texture, and also partly by the abundance of the major constituent minerals in the ore, being pyroxenes, base metal sulfides, and chromite. Liberation of pentlandite is directly correlated with grind size (milling time), which is also positively correlated with the level of nickel recovery through flotation. A grind size of P80 at 75 µm results in the highest concentrate nickel grades of 7.5–8.1% in the PCMZ ores’ types which is the current grind for the PCMZ ores at Nkomati. A grind size of P77 at 75 µm yields the best overall pentlandite liberation, Ni recoveries of 84–88% and grades of 5.3–5.6% in the MMZ ores. This holds the potential to produce the best overall pentlandite liberation, nickel grades, recoveries from blending the MMZ and PCMZ ore types, and milling the composite ore at a target grind of P80 at 75 µm.
Peculiarities of Dynamics of Hypergenic Mineral Transformation of Nickel Weathering Crusts of Ultramafic Rocks of the Kempirsay Group of Deposits in Western Kazakhstan
Authors: Korobkin Valeriy , Samatov Iskander , Chaklikov Akhan , Tulemissova Zhamal
Published: May 2022

Nickel weathering ores are used to produce metallic nickel, stainless steels, and nickel sulfate, the main component of batteries. The global production of nickel from weathering ores is increasing and has surpassed production from sulfide magmatic deposits. The efficiency of the mining and processing of nickel ores from weathering rocks is determined by their mineralogical composition. The weathering crust profile of the Kempirsay ultramafite massif is divided into three zones—leached (kerolitized) serpentinites, nontronites, and final hydrolysis minerals (later referred to as “ochers”). The kerolitized zone consists of a mixture of Ni-bearing talc and saponites (later referred to as “kerolite”). During the geological mapping of the Donskoye, Buranovskoye, and Shelektinskoye deposits, the products of ultramafite hypergenic transformation into disintegrated and leached serpentinites, kerolites, nontronites, and ochers were selected and studied. For this purpose, 44 rock samples were studied via X-ray diffractometric and thermal analyses, supplemented with data from chemical, microscopic, and granulometric determinations. Based on the obtained numerical parameters of the crystalline structure of the weathering products, the thermochemical values were obtained. The hypergenic transformation of the initial minerals and their subsequent transformation were traced. The trace element distribution along the profile of the serpentinite weathering ores is related to the initial material composition of the ultramafites. The accumulation of nickel in industrial concentrations is associated with the nontronite–kerolite zone. X-ray diffractometric analysis can be used as a fast and reliable method for controlling the nickel content of ores and monitoring their mineralogical composition.
Pharmacokinetics, Prostate Distribution and Metabolic Characteristics of Four Representative Flavones after Oral Administration of the Aerial Part of Glycyrrhiza uralensis in Rats
Authors: Liu Haifan , Chang Guanhua , Wang Wenquan , Ji Zuen , Cui Jie , Peng Yifeng
Published: May 2022

(1) Background: The aerial part of G. uralensis had pharmacological effects against chronic non-bacterial prostatitis (CNP), and flavonoids are the main efficacy components. The purpose of this study was to obtain the pharmacokinetics, prostate distribution and metabolic characteristics of some flavonoids in rats. (2) Methods: The prototype flavones and the metabolites of four representative flavonoids, namely puerarin, luteolin, kaempferol and pinocembrin in plasma, prostate, urine and feces of rats were analyzed by UPLC-Q-Exactive Orbitrap-MS. In addition, the pharmacokinetic parameters in plasma and distribution of prostate of four components were analyzed by HPLC-MS/MS. (3) Results: In total, 22, 17, 22 and 11 prototype flavones were detected in the prostate, plasma, urine and feces, respectively. The metabolites of puerarin in the prostate are hydrolysis and glucose-conjugated products, the metabolites of kaempferol and luteolin in the prostate are methylation and glucuronidation, and the metabolites of pinocembrin in the prostate are naringenin, oxidation, sulfation, methylation and glucuronidation products. The t1/2 of puerarin, luteolin, kaempferol and pinocembrin was 6.43 ± 0.20, 31.08 ± 1.17, 18.98 ± 1.46 and 13.18 ± 0.72 h, respectively. The concentrations of the four flavonoids in prostate were ranked as kaempferol > pinocembrin > luteolin > puerarin. (4) Conclusions: Methylation and glucuronidation metabolites were the main metabolites detected in the prostate. A sensitive and validated HPLC–MS/MS method for simultaneous determination of puerarin, luteolin, kaempferol and pinocembrin in rat plasma and prostate was described, and it was successfully applied to the pharmacokinetic and prostate distribution studies.
Anion Binding by Fluorescent Ureido-Hexahomotrioxacalix[3]arene Receptors: An NMR, Absorption and Emission Spectroscopic Study
Authors: Miranda Alexandre S. , Marcos Paula M. , Ascenso José R. , Berberan-Santos Mário N. , Menezes Filipe
Published: May 2022

Fluorescent receptors (4a–4c) based on (thio)ureido-functionalized hexahomotrioxacalix[3]arenes were synthesised and obtained in the partial cone conformation in solution. Naphthyl or pyrenyl fluorogenic units were introduced at the lower rim of the calixarene skeleton via a butyl spacer. The binding of biologically and environmentally relevant anions was studied with NMR, UV–vis absorption, and fluorescence titrations. Fluorescence of the pyrenyl receptor 4c displays both monomer and excimer fluorescence. The thermodynamics of complexation was determined in acetonitrile and was entropy-driven. Computational studies were also performed to bring further insight into the binding process. The data showed that association constants increase with the anion basicity, and AcO−, BzO− and F− were the best bound anions for all receptors. Pyrenylurea 4c is a slightly better receptor than naphthylurea 4a, and both are more efficient than naphthyl thiourea 4b. In addition, ureas 4a and 4c were also tested as ditopic receptors in the recognition of alkylammonium salts.
Microarray Analysis of the Genomic Effect of Eugenol on Methicillin-Resistant Staphylococcus aureus
Authors: Buru Ayuba Sunday , Neela Vasantha Kumari , Mohandas Kavitha , Pichika Mallikarjuna Rao
Published: May 2022

Staphylococcus aureus is a highly adaptive human pathogen responsible for serious hospital- and community-acquired infectious diseases, ranging from skin and soft tissue infections, to complicated and life-threatening conditions such as endocarditis and toxic shock syndrome (TSS). The rapid development of resistance of this organism to available antibiotics over the last few decades has necessitated a constant search for more efficacious antibacterial agents. Eugenol (4-allyl-2-methoxyphenol) belongs to the class of chemical compounds called phenylpropanoids. It is a pure-to-pale yellow, oily liquid substance, mostly extracted as an essential oil from natural products such as clove, cinnamon, nutmeg, basil, and bay leaf. Eugenol has previously been shown to have antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). However, the mechanism of action of eugenol against MRSA has not, as yet, been elucidated; hence, the necessity of this study. Global gene expression patterns in response to challenge from subinhibitory concentrations of eugenol were analysed using the Agilent DNA microarray system to identify genes that can be used as drug targets—most importantly, essential genes involved in unique metabolic pathways elicited for bacterial survival. Transcriptomic analysis of fluctuating genes revealed those involved in amino acid metabolism, fatty acid metabolism, translational, and ribosomal pathways. In amino acid metabolism, for instance, the argC gene encodes for N-acetyl-gamma-glutamyl-phosphate reductase. The argC gene plays an important role in the biosynthesis of arginine from glutamate in the amino acid metabolic pathway. It is the enzyme that catalyses the third step in the latter reaction, and without this process the production of N-acetylglutamate 5-semialdehyde cannot be completed from the NADP-dependent reduction of N-acetyl-5-glutamyl phosphate, which is essential for the survival of some microorganisms and plants. This study enables us to examine complete global transcriptomic responses in MRSA when challenged with eugenol. It reveals novel information with the potential to further benefit the exploratory quest for novel targets against this pathogen, with a view to the development of efficacious antimicrobial agents for the treatment of associated infections.
Effects of Spiro-Cyclohexane Substitution of Nitroxyl Biradicals on Dynamic Nuclear Polarization
Authors: Asanbaeva Nargiz B. , Gurskaya Larisa Yu. , Polienko Yuliya F. , Rybalova Tatyana V. , Kazantsev Maxim S. , Dmitriev Alexey A. , Gritsan Nina P. , Haro-Mares Nadia , Gutmann Torsten , Buntkowsky Gerd , Tretyakov Evgeny V. , Bagryanskaya Elena G.
Published: May 2022

Spiro-substituted nitroxyl biradicals are widely used as reagents for dynamic nuclear polarization (DNP), which is especially important for biopolymer research. The main criterion for their applicability as polarizing agents is the value of the spin–spin exchange interaction parameter (J), which can vary considerably when different couplers are employed that link the radical moieties. This paper describes a study on biradicals, with a ferrocene-1,1′-diyl-substituted 1,3-diazetidine-2,4-diimine coupler, that have never been used before as DNP agents. We observed a substantial difference in the temperature dependence between Electron Paramagnetic Resonance (EPR) spectra of biradicals carrying either methyl or spirocyclohexane substituents and explain the difference using Density Functional Theory (DFT) calculation results. It was shown that the replacement of methyl groups by spirocycles near the N-O group leads to an increase in the contribution of conformers having J ≈ 0. The DNP gain observed for the biradicals with methyl substituents is three times higher than that for the spiro-substituted nitroxyl biradicals and is inversely proportional to the contribution of biradicals manifesting the negligible exchange interaction. The effects of nucleophiles and substituents in the nitroxide biradicals on the ring-opening reaction of 1,3-diazetidine and the influence of the ring opening on the exchange interaction were also investigated. It was found that in contrast to the methyl-substituted nitroxide biradical (where we observed the ring-opening reaction upon the addition of amines), the ring opening does not occur in the spiro-substituted biradical owing to a steric barrier created by the bulky cyclohexyl substituents.
Development of a Solid-Phase Extraction Method Based on Biocompatible Starch Polyurethane Polymers for GC-MS Analysis of Polybrominated Diphenyl Ethers in Ambient Water Samples
Authors: Zhang Qian , Okoli Chukwunonso P.
Published: May 2022

A new solid-phase extraction (SPE) method for the extraction, enrichment, and analysis of eight polybrominated diphenyl ethers (PBDEs) in water was developed. The current approach involves using a cross-linked starch-based polymer as an extraction adsorbent and determining the PBDE analytes of interest using gas chromatography-mass spectrometry in negative chemical ionization mode (GC-NCI-MS). The starch-based polymer was synthesized by the reaction of soluble starch with 4,4′-methylene-bis-phenyldiisocyanate as a cross-linking agent in dry dimethylformamide. Various parameters impacting extraction efficiencies, such as adsorbent quantity, sample volumes, elution solvents and volumes, and methanol content, were carefully optimized. The 500 mg of starch-based polymer as an adsorbent used to extract 1000 mL of spiked water, presented high extraction recoveries of eight PBDEs. The linearity of the extraction process was investigated in the range of 1–200 ng L−1 for BDE-28, 47, 99, 100, and 5–200 ng L−1 for BDE-153, 154, 183, and 209, with coefficients of determination (r2) exceeding 0.990 for all PBDEs. The limits of detection (LODs) ranged from 0.06 to 1.42 ng L−1 (S/N = 3) and the relative standard deviation values (RSD) were between 3.6 and 9.5 percent (n = 5) under optimum conditions. The method was successfully used to analyze river and lake water samples, where it exhibited acceptable recovery values of 71.3 to 104.2%. Considering the excellent analytical performance and comparative cost advantage, we recommend the developed starch-based SPE method for routine extraction and analysis of PBDEs in water media.
Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin
Authors: Marcantonini Giada , Bartolini Desirée , Zatini Linda , Costa Stefania , Passerini Massimiliano , Rende Mario , Luca Giovanni , Basta Giuseppe , Murdolo Giuseppe , Calafiore Riccardo , Galli Francesco
Published: May 2022

Cryoprotective and cytoprotective agents (Cytoprotective Agents) are fundamental components of the cryopreservation process. This review presents the essentials of the cryopreservation process by examining its drawbacks and the role of cytoprotective agents in protecting cell physiology. Natural cryoprotective and cytoprotective agents, such as antifreeze proteins, sugars and natural deep eutectic systems, have been compared with synthetic ones, addressing their mechanisms of action and efficacy of protection. The final part of this article focuses melatonin, a hormonal substance with antioxidant properties, and its emerging role as a cytoprotective agent for somatic cells and gametes, including ovarian tissue, spermatozoa and spermatogonial stem cells.