bluuebunny/crossref_metadata_2025 huggingface dataset:
Hypoxia-Inducible Factor-1: A Novel Therapeutic Target for the Management of Cancer, Drug Resistance, and Cancer-Related Pain
Authors: Bui Bich Phuong , Nguyen Phuong Linh , Lee Kyeong , Cho Jungsook
Published: December 2022

Hypoxia-inducible factor-1 (HIF-1) is a key transcription factor that regulates the transcription of many genes that are responsible for the adaptation and survival of tumor cells in hypoxic environments. Over the past few decades, tremendous efforts have been made to comprehensively understand the role of HIF-1 in tumor progression. Based on the pivotal roles of HIF-1 in tumor biology, many HIF-1 inhibitors interrupting expression, stabilization, DNA binding properties, or transcriptional activity have been identified as potential therapeutic agents for various cancers, yet none of these inhibitors have yet been successfully translated into clinically available cancer treatments. In this review, we briefly introduce the regulation of the HIF-1 pathway and summarize its roles in tumor cell proliferation, angiogenesis, and metastasis. In addition, we explore the implications of HIF-1 in the development of drug resistance and cancer-related pain: the most commonly encountered obstacles during conventional anticancer therapies. Finally, the current status of HIF-1 inhibitors in clinical trials and their perspectives are highlighted, along with their modes of action. This review provides new insights into novel anticancer drug development targeting HIF-1. HIF-1 inhibitors may be promising combinational therapeutic interventions to improve the efficacy of current cancer treatments and reduce drug resistance and cancer-related pain.
T-Cell Mediated Immunity in Merkel Cell Carcinoma
Authors: Ouyang Kelsey , Zheng David X. , Agak George W.
Published: December 2022

Merkel cell carcinoma (MCC) is a rare and frequently lethal skin cancer with neuroendocrine characteristics. MCC can originate from either the presence of MCC polyomavirus (MCPyV) DNA or chronic ultraviolet (UV) exposure that can cause DNA mutations. MCC is predominant in sun-exposed regions of the body and can metastasize to regional lymph nodes, liver, lungs, bone, and brain. Older, light-skinned individuals with a history of significant sun exposure are at the highest risk. Previous studies have shown that tumors containing a high number of tumor-infiltrating T-cells have favorable survival, even in the absence of MCPyV DNA, suggesting that MCPyV infection enhances T-cell infiltration. However, other factors may also play a role in the host antitumor response. Herein, we review the impact of tumor infiltrating lymphocytes (TILs), mainly the CD4+, CD8+, and regulatory T-cell (Tregs) responses on the course of MCC, including their role in initiating MCPyV-specific immune responses. Furthermore, potential research avenues related to T-cell biology in MCC, as well as relevant immunotherapies are discussed.
1-Methylimidazole as an Organic Catalyst for [3+3]-Cyclodimerization of Acylethynylpyrroles to Bis(acylmethylidene)dipyrrolo[1,2-a:1′,2′-d]pyrazines
Authors: Belyaeva Kseniya V. , Nikitina Lina P. , Gen’ Veronika S. , Tomilin Denis N. , Sobenina Lyubov N. , Afonin Andrei V. , Oparina Ludmila A. , Trofimov Boris A.
Published: December 2022

Acylethynylpyrroles, now readily available by the cross-coupling of pyrroles with acylbromoacetylenes in solid Al2O3 media, in the presence of 1-methylimidazole underwent unprecedentedly easy (40–45 °C) cyclodimerization into bis(acylmethylidene)dipyrrolo[1,2-a:1′,2′-d]pyrazines in up to 51% yield. Some other organic and inorganic basic catalysts can also trigger this cyclodimerization, but less efficiently.
Zeolitic Imidazolate Framework Decorated Molybdenum Carbide Catalysts for Hydrodeoxygenation of Guaiacol to Phenol
Authors: Kurisingal Jintu Francis , Lee Shinjae , Lee Jun Gyeong , An Kwangjin
Published: December 2022

Bimetallic zeolitic imidazolate framework (BMZIF)-decorated Mo carbide catalysts were designed for the catalytic hydrodeoxygenation of guaiacol to produce phenol with high selectivity. A uniform layer of BMZIF was systematically coated onto the surface of the MoO3 nanorods. During carbonization at 700 °C for 4 h, BMZIF generated active species (ZnO, CoO) on highly dispersed N-doped carbons, creating a porous shell structure. Simultaneously, the MoO3 nanorod was transformed into the Mo2C phase. The resulting core@shell type Mo2C@BMZIF-700 °C (4 h) catalyst promoted a 97% guaiacol conversion and 70% phenol selectivity under 4 MPa of H2 at 330 °C for 4 h, which was not achieved by other supported catalysts. The catalyst also showed excellent selective cleavage of the methoxy group of lignin derivatives (syringol and vanillin), which makes it suitable for selective demethoxylation in future biomass catalysis. Moreover, it exhibits excellent recyclability and stability without changing the structure or active species.
Catalytic Ozonation of Norfloxacin Using Co-Mn/CeO2 as a Multi-Component Composite Catalyst
Authors: Li Ruicheng , Xiong Jianhua , Zhang Yuanyuan , Wang Shuangfei , Zhu Hongxiang , Lu Lihai
Published: December 2022

In this study, a Co-Mn/CeO2 composite was prepared through a facile sol-gel method and used as an efficient catalyst for the ozonation of norfloxacin (NOR). The Co-Mn/CeO2 composite was characterized via XRD, SEM, BET and XPS analysis. The catalytic ozonation of NOR by Co-Mn/CeO2 under different conditions was systematically investigated, including the effect of the initial solution’s pH, Co-Mn/CeO2 composite dose, O3 dose and NOR concentration on degradation kinetics. Only about 3.33% of total organic carbon (TOC) and 72.17% of NOR could be removed within 150 min by single ozonation under the conditions of 60 mg/L of NOR and 200 mL/min of O3 at pH= 7 and room temperature, whereas in the presence of 0.60 g/L of the Co-Mn/CeO2 composite under the same conditions, 87.24% NOR removal was obtained through the catalytic ozonation process. The results showed that catalytic ozonation with the Co-Mn/CeO2 composite could effectively enhance the degradation and mineralization of NOR compared to a single ozonation system alone. The catalytic performance of CeO2 was significantly improved by the modification with Mn and Co. Co-Mn/CeO2 represents a promising way to prepare efficient catalysts for the catalytic ozonation of organic polluted water. The removal efficiency of NOR in five cycles indicates that Co-Mn/CeO2 is stable and recyclable for catalytic ozonation in water treatment.
Human In Vitro Models of Epilepsy Using Embryonic and Induced Pluripotent Stem Cells
Authors: Javaid Muhammad Shahid , Tan Tracie , Dvir Naomi , Anderson Alison , J. O’Brien Terence , Kwan Patrick , Antonic-Baker Ana
Published: December 2022

The challenges in making animal models of complex human epilepsy phenotypes with varied aetiology highlights the need to develop alternative disease models that can address the limitations of animal models by effectively recapitulating human pathophysiology. The advances in stem cell technology provide an opportunity to use human iPSCs to make disease-in-a-dish models. The focus of this review is to report the current information and progress in the generation of epileptic patient-specific iPSCs lines, isogenic control cell lines, and neuronal models. These in vitro models can be used to study the underlying pathological mechanisms of epilepsies, anti-seizure medication resistance, and can also be used for drug testing and drug screening with their isogenic control cell lines.
In Skeletal Muscle Fibers, Protein Kinase Subunit CSNK2A1/CK2α Is Required for Proper Muscle Homeostasis and Structure and Function of Neuromuscular Junctions
Authors: Merholz Mira , Jian Yongzhi , Wimberg Johannes , Gessler Lea , Hashemolhosseini Said
Published: December 2022

CSNK2 tetrameric holoenzyme is composed of two subunits with catalytic activity (CSNK2A1 and/or CSNK2A2) and two regulatory subunits (CSNK2B) and is involved in skeletal muscle homeostasis. Up-to-date, constitutive Csnk2a2 knockout mice demonstrated mild regenerative impairments in skeletal muscles, while conditional Csnk2b mice were linked to muscle weakness, impaired neuromuscular transmission, and metabolic and autophagic compromises. Here, for the first time, skeletal muscle-specific conditional Csnk2a1 mice were generated and characterized. The ablation of Csnk2a1 expression was ensured using a human skeletal actin-driven Cre reporter. In comparison with control mice, first, conditional knockout of CSNK2A1 resulted in age-dependent reduced grip strength. Muscle weakness was accompanied by impaired neuromuscular transmission. Second, the protein amount of other CSNK2 subunits was aberrantly changed. Third, the number of central nuclei in muscle fibers indicative of regeneration increased. Fourth, oxidative metabolism was impaired, reflected by an increase in cytochrome oxidase and accumulation of mitochondrial enzyme activity underneath the sarcolemma. Fifth, autophagic processes were stimulated. Sixth, NMJs were fragmented and accompanied by increased synaptic gene expression levels. Altogether, knockout of Csnk2a1 or Csnk2b results in diverse impairments of skeletal muscle biology.
Protective Biomolecular Mechanisms of Glutathione Sodium Salt in Ischemia-Reperfusion Injury in Patients with Acute Coronary Syndrome-ST-Elevation Myocardial Infarction
Authors: Arrivi Alessio , Barillà Francesco , Carnevale Roberto , Sordi Martina , Pucci Giacomo , Tanzilli Gaetano , Prandi Francesca Romana , Mangieri Enrico
Published: December 2022

Ischemia-Reperfusion Injury (IRI) is responsible for adverse outcomes in patients with ST-Elevation Myocardial Infarction (STEMI). Oxidative stress, resulting from the production of Reactive Oxygen Species (ROS) and low availability of Glutathione (GSH), are the two main mediators of IRI. The effectiveness of exogenous antioxidant therapy in this scenario is still debated, since the encouraging results obtained in animal models have not been fully reproduced in clinical studies. In this review we focus on the role of GSH, specifically on the biomolecular mechanisms that preserve myocardial cells from damage due to reperfusion. In this regard, we provide an extensive discussion about GSH intrinsic antioxidant properties, its current applications in clinical practice, and the future perspectives.
Artificial Intelligence and Advanced Melanoma: Treatment Management Implications
Authors: Guerrisi Antonino , Falcone Italia , Valenti Fabio , Rao Marco , Gallo Enzo , Ungania Sara , Maccallini Maria Teresa , Fanciulli Maurizio , Frascione Pasquale , Morrone Aldo , Caterino Mauro
Published: December 2022

Artificial intelligence (AI), a field of research in which computers are applied to mimic humans, is continuously expanding and influencing many aspects of our lives. From electric cars to search motors, AI helps us manage our daily lives by simplifying functions and activities that would be more complex otherwise. Even in the medical field, and specifically in oncology, many studies in recent years have highlighted the possible helping role that AI could play in clinical and therapeutic patient management. In specific contexts, clinical decisions are supported by “intelligent” machines and the development of specific softwares that assist the specialist in the management of the oncology patient. Melanoma, a highly heterogeneous disease influenced by several genetic and environmental factors, to date is still difficult to manage clinically in its advanced stages. Therapies often fail, due to the establishment of intrinsic or secondary resistance, making clinical decisions complex. In this sense, although much work still needs to be conducted, numerous evidence shows that AI (through the processing of large available data) could positively influence the management of the patient with advanced melanoma, helping the clinician in the most favorable therapeutic choice and avoiding unnecessary treatments that are sure to fail. In this review, the most recent applications of AI in melanoma will be described, focusing especially on the possible finding of this field in the management of drug treatments.
Deep Diversity: Extensive Variation in the Components of Complex Visual Systems across Animals
Authors: Vöcking Oliver , Macias-Muñoz Aide , Jaeger Stuart J. , Oakley Todd H.
Published: December 2022

Understanding the molecular underpinnings of the evolution of complex (multi-part) systems is a fundamental topic in biology. One unanswered question is to what the extent do similar or different genes and regulatory interactions underlie similar complex systems across species? Animal eyes and phototransduction (light detection) are outstanding systems to investigate this question because some of the genetics underlying these traits are well characterized in model organisms. However, comparative studies using non-model organisms are also necessary to understand the diversity and evolution of these traits. Here, we compare the characteristics of photoreceptor cells, opsins, and phototransduction cascades in diverse taxa, with a particular focus on cnidarians. In contrast to the common theme of deep homology, whereby similar traits develop mainly using homologous genes, comparisons of visual systems, especially in non-model organisms, are beginning to highlight a “deep diversity” of underlying components, illustrating how variation can underlie similar complex systems across taxa. Although using candidate genes from model organisms across diversity was a good starting point to understand the evolution of complex systems, unbiased genome-wide comparisons and subsequent functional validation will be necessary to uncover unique genes that comprise the complex systems of non-model groups to better understand biodiversity and its evolution.
Synergistic Effect of Static Magnetic Fields and 3D-Printed Iron-Oxide-Nanoparticle-Containing Calcium Silicate/Poly-ε-Caprolactone Scaffolds for Bone Tissue Engineering
Authors: Kao Chuan-Yi , Lin Tsung-Li , Lin Yen-Hong , Lee Alvin Kai-Xing , Ng Sing Yee , Huang Tsui-Hsien , Hsu Tuan-Ti
Published: December 2022

In scaffold-regulated bone regeneration, most three-dimensional (3D)-printed scaffolds do not provide physical stimulation to stem cells. In this study, a magnetic scaffold was fabricated using fused deposition modeling with calcium silicate (CS), iron oxide nanoparticles (Fe3O4), and poly-ε-caprolactone (PCL) as the matrix for internal magnetic sources. A static magnetic field was used as an external magnetic source. It was observed that 5% Fe3O4 provided a favorable combination of compressive strength (9.6 ± 0.9 MPa) and degradation rate (21.6 ± 1.9% for four weeks). Furthermore, the Fe3O4-containing scaffold increased in vitro bioactivity and Wharton’s jelly mesenchymal stem cells’ (WJMSCs) adhesion. Moreover, it was shown that the Fe3O4-containing scaffold enhanced WJMSCs’ proliferation, alkaline phosphatase activity, and the osteogenic-related proteins of the scaffold. Under the synergistic effect of the static magnetic field, the CS scaffold containing Fe3O4 can not only enhance cell activity but also stimulate the simultaneous secretion of collagen I and osteocalcin. Overall, our results demonstrated that Fe3O4-containing CS/PCL scaffolds could be fabricated three dimensionally and combined with a static magnetic field to affect cell behaviors, potentially increasing the likelihood of clinical applications for bone tissue engineering.
In Adult Skeletal Muscles, the Co-Receptors of Canonical Wnt Signaling, Lrp5 and Lrp6, Determine the Distribution and Size of Fiber Types, and Structure and Function of Neuromuscular Junctions
Authors: Gessler Lea , Kurtek Christopher , Merholz Mira , Jian Yongzhi , Hashemolhosseini Said
Published: December 2022

Canonical Wnt signaling is involved in skeletal muscle cell biology. The exact way in which this pathway exerts its contribution to myogenesis or neuromuscular junctions (NMJ) is a matter of debate. Next to the common co-receptors of canonical Wnt signaling, Lrp5 and Lrp6, the receptor tyrosine kinase MuSK was reported to bind at NMJs WNT glycoproteins by its extracellular cysteine-rich domain. Previously, we reported canonical Wnt signaling being active in fast muscle fiber types. Here, we used conditional Lrp5 or Lrp6 knockout mice to investigate the role of these receptors in muscle cells. Conditional double knockout mice died around E13 likely due to ectopic expression of the Cre recombinase. Phenotypes of single conditional knockout mice point to a very divergent role for the two receptors. First, muscle fiber type distribution and size were changed. Second, canonical Wnt signaling reporter mice suggested less signaling activity in the absence of Lrps. Third, expression of several myogenic marker genes was changed. Fourth, NMJs were of fragmented phenotype. Fifth, recordings revealed impaired neuromuscular transmission. In sum, our data show fundamental differences in absence of each of the Lrp co-receptors and suggest a differentiated view of canonical Wnt signaling pathway involvement in adult skeletal muscle cells.
A New Method to Sort Differentiating Osteoclasts into Defined Homogeneous Subgroups
Authors: Hulley Philippa A. , Knowles Helen J.
Published: December 2022

Osteoclasts regulate skeletal development but also drive pathological osteolysis, making them prime therapeutic targets. Osteoclast research is limited by the heterogeneity of osteoclast populations generated in vitro, where the mixture of undifferentiated monocytes, binuclear pre-osteoclasts and multinucleated osteoclasts has by necessity been considered a single osteoclast population. This study describes the differentiation of primary human CD14+ monocyte-derived osteoclasts in 3D collagen gels. These osteoclasts remained small (>95% with ≤5 nuclei) but were viable and active; when released from the gel with collagenase, they fused rapidly when reseeded onto solid substrates and resorbed dentine for 2–3 weeks. 3D-generated osteoclasts expressed cell surface markers of osteoclast differentiation (e.g., CD9, RANK, OSCAR, CD63, CD51/61) which, with their small size, enabled live cell sorting of highly enriched viable subpopulations of human osteoclasts that retained full functional resorption capacity. Low-yield osteoclast preparations were strongly enriched to remove undifferentiated cells (e.g., 13.3% CD51/61+ to 84.2% CD51/61+), and subpopulations of CD9+CD51/61− early osteoclasts and CD9+CD51/61+ mature cells were distinguished. This novel approach allows the study of selected populations of differentiating osteoclasts in vitro and opens the door to in-depth transcriptomic and proteomic analysis of these cells, increasing our ability to study human osteoclast molecular mechanisms relevant to development, aging and disease.
Effect of Mechanical Loading of Senescent Myoblasts on Their Myogenic Lineage Progression and Survival
Authors: Moustogiannis Athanasios , Philippou Anastassios , Zevolis Evangelos , Taso Orjona S. , Giannopoulos Antonios , Chatzigeorgiou Antonios , Koutsilieris Michael
Published: December 2022

Background: During aging, muscle cell apoptosis increases and myogenesis gradually declines. The impaired myogenic and survival potential of the aged skeletal muscle can be ameliorated by its mechanical loading. However, the molecular responses of aged muscle cells to mechanical loading remain unclear. This study examined the effect of mechanical loading of aged, proliferating, and differentiated myoblasts on the gene expression and signaling responses associated with their myogenic lineage progression and survival. Methods: Control and aged C2C12 cells were cultured on elastic membranes and underwent passive stretching for 12 h at a low frequency (0.25 Hz) and different elongations, varying the strain on days 0 and 10 of myoblast differentiation. Activation of ERK1/2 and Akt, and the expression of focal adhesion kinase (FAK) and key myogenic regulatory factors (MRFs), MyoD and Myogenin, were determined by immunoblotting of the cell lysates derived from stretched and non-stretched myoblasts. Changes in the expression levels of the MRFs, muscle growth, atrophy, and pro-apoptotic factors in response to mechanical loading of the aged and control cells were quantified by real-time qRT-PCR. Results: Mechanical stretching applied on myoblasts resulted in the upregulation of FAK both in proliferating (day 0) and differentiated (day 10) cells, as well as in increased phosphorylation of ERK1/2 in both control and aged cells. Moreover, Akt activation and the expression of early differentiation factor MyoD increased significantly after stretching only in the control myoblasts, while the late differentiation factor Myogenin was upregulated in both the control and aged myoblasts. At the transcriptional level, mechanical loading of the proliferating myoblasts led to an increased expression of IGF-1 isoforms and MRFs, and to downregulation of muscle atrophy factors mainly in control cells, as well as in the upregulation of pro-apoptotic factors both in control and aged cells. In differentiated cells, mechanical loading resulted in an increased expression of the IGF-1Ea isoform and Myogenin, and in the downregulation of atrophy and pro-apoptotic factors in both the control and aged cells. Conclusions: This study revealed a diminished beneficial effect of mechanical loading on the myogenic and survival ability of the senescent muscle cells compared with the controls, with a low strain (2%) loading being most effective in upregulating myogenic/anabolic factors and downregulating atrophy and pro-apoptotic genes mainly in the aged myotubes.
Independent Responses of Photosynthesis and Plant Morphology to Alterations of PIF Proteins and Light-Dependent MicroRNA Contents in Arabidopsis thaliana pif Mutants Grown under Lights of Different Spectral Compositions
Authors: Pashkovskiy Pavel , Kreslavski Vladimir , Khudyakova Alexandra , Pojidaeva Elena S. , Kosobryukhov Anatoliy , Kuznetsov Vladimir , Allakhverdiev Suleyman I.
Published: December 2022

The effects of the quality of light on the content of phytochrome interacting factors (PIFs) such as PIF3, PIF4 and PIF5, as well as the expression of various light-dependent microRNAs, in adult Arabidopsis thaliana pif mutant plants (pif4, pif5, pif3pif5, pif4pif5, pif3pif4pif5) were studied. We demonstrate that under blue light, the pif4 mutant had maximal expression of most of the studied microRNAs (miR163, miR319, miR398, miR408, miR833) when the PIF4 protein in plants was reduced. This finding indicates that the PIF4 protein is involved in the downregulation of this group of microRNAs. This assumption is additionally confirmed by the fact that under the RL spectrum in pif5 mutants, practically the same miRNAs decrease expression against the background of an increase in the amount of PIF4 protein. Unlike the WT and other mutants, the pif4 mutant responded to the BL spectrum not only by activating the expression of light-dependent miRNAs, but also by a significant increase in the expression of transcription factors and key light signalling genes. These molecular reactions do not affect the activity of photosynthesis but may be involved in the formation of a light quality-dependent phenotype.
Loss of Pex1 in Inner Ear Hair Cells Contributes to Cochlear Synaptopathy and Hearing Loss
Authors: Mauriac Stephanie A. , Peineau Thibault , Zuberi Aamir , Lutz Cathleen , Géléoc Gwénaëlle S. G.
Published: December 2022

Peroxisome Biogenesis Disorders (PBD) and Zellweger syndrome spectrum disorders (ZSD) are rare genetic multisystem disorders that include hearing impairment and are associated with defects in peroxisome assembly, function, or both. Mutations in 13 peroxin (PEX) genes have been found to cause PBD-ZSD with ~70% of patients harboring mutations in PEX1. Limited research has focused on the impact of peroxisomal disorders on auditory function. As sensory hair cells are particularly vulnerable to metabolic changes, we hypothesize that mutations in PEX1 lead to oxidative stress affecting hair cells of the inner ear, subsequently resulting in hair cell degeneration and hearing loss. Global deletion of the Pex1 gene is neonatal lethal in mice, impairing any postnatal studies. To overcome this limitation, we created conditional knockout mice (cKO) using Gfi1Creor VGlut3Cre expressing mice crossed to floxed Pex1 mice to allow for selective deletion of Pex1 in the hair cells of the inner ear. We find that Pex1 excision in inner hair cells (IHCs) leads to progressive hearing loss associated with significant decrease in auditory brainstem responses (ABR), specifically ABR wave I amplitude, indicative of synaptic defects. Analysis of IHC synapses in cKO mice reveals a decrease in ribbon synapse volume and functional alterations in exocytosis. Concomitantly, we observe a decrease in peroxisomal number, indicative of oxidative stress imbalance. Taken together, these results suggest a critical function of Pex1 in development and maturation of IHC-spiral ganglion synapses and auditory function.
Impact of Liver Inflammation on Bile Acid Side Chain Shortening and Amidation
Authors: Alonso-Peña Marta , Espinosa-Escudero Ricardo , Hermanns Heike M. , Briz Oscar , Herranz Jose M. , Garcia-Ruiz Carmen , Fernandez-Checa Jose C. , Juamperez Javier , Avila Matias , Argemi Josepmaria , Bataller Ramon , Crespo Javier , Monte Maria J. , Geier Andreas , Herraez Elisa , Marin Jose J. G.
Published: December 2022

Bile acid (BA) synthesis from cholesterol by hepatocytes is inhibited by inflammatory cytokines. Whether liver inflammation also affects BA side chain shortening and conjugation was investigated. In human liver cell lines (IHH, HepG2, and HepaRG), agonists of nuclear receptors including the farnesoid X receptor (FXR), liver X receptor (LXR), and peroxisome proliferator-activated receptors (PPARs) did not affect the expression of BA-related peroxisomal enzymes. In contrast, hepatocyte nuclear factor 4α (HNF4α) inhibition down-regulated acyl-CoA oxidase 2 (ACOX2). ACOX2 was repressed by fibroblast growth factor 19 (FGF19), which was prevented by extracellular signal-regulated kinase (ERK) pathway inhibition. These changes were paralleled by altered BA synthesis (HPLC-MS/MS). Cytokines able to down-regulate cholesterol-7α-hydroxylase (CYP7A1) had little effect on peroxisomal enzymes involved in BA synthesis except for ACOX2 and bile acid-CoA:amino acid N-acyltransferase (BAAT), which were down-regulated, mainly by oncostatin M (OSM). This effect was prevented by Janus kinase (JAK) inhibition, which restored BA side chain shortening and conjugation. The binding of OSM to the extracellular matrix accounted for a persistent effect after culture medium replacement. In silico analysis of four databases (n = 201) and a validation cohort (n = 90) revealed an inverse relationship between liver inflammation and ACOX2/BAAT expression which was associated with changes in HNF4α levels. In conclusion, BA side chain shortening and conjugation are inhibited by inflammatory effectors. However, other mechanisms involved in BA homeostasis counterbalance any significant impact on the serum BA profile.
Prediction Model for Identifying Factors Associated with Epilepsy in Children with Cerebral Palsy
Authors: Bertoncelli Carlo Mario , Dehan Nathalie , Bertoncelli Domenico , Bagui Sikha , Bagui Subhash C. , Costantini Stefania , Solla Federico
Published: December 2022

(1) Background: Cerebral palsy (CP) is associated with a higher incidence of epileptic seizures. This study uses a prediction model to identify the factors associated with epilepsy in children with CP. (2) Methods: This is a retrospective longitudinal study of the clinical characteristics of 102 children with CP. In the study, there were 58 males and 44 females, 65 inpatients and 37 outpatients, 72 had epilepsy, and 22 had intractable epilepsy. The mean age was 16.6 ± 1.2 years, and the age range for this study was 12–18 years. Data were collected on the CP etiology, diagnosis, type of epilepsy and spasticity, clinical history, communication abilities, behaviors, intellectual disability, motor function, and feeding abilities from 2005 to 2020. A prediction model, Epi-PredictMed, was implemented to forecast the factors associated with epilepsy. We used the guidelines of “Transparent Reporting of a multivariable prediction model for Individual Prognosis or Diagnosis” (TRIPOD). (3) Results: CP etiology [(prenatal > perinatal > postnatal causes) p = 0.036], scoliosis (p = 0.048), communication (p = 0.018), feeding disorders (p = 0.002), poor motor function (p < 0.001), intellectual disabilities (p = 0.007), and the type of spasticity [(quadriplegia/triplegia > diplegia > hemiplegia), p = 0.002)] were associated with having epilepsy. The model scored an average of 82% for accuracy, sensitivity, and specificity. (4) Conclusion: Prenatal CP etiology, spasticity, scoliosis, severe intellectual disabilities, poor motor skills, and communication and feeding disorders were associated with epilepsy in children with CP. To implement preventive and/or management measures, caregivers and families of children with CP and epilepsy should be aware of the likelihood that these children will develop these conditions.
How COVID-19 Phases Have Impacted Psychiatric Risk: A Retrospective Study in an Emergency Care Unit for Adolescents
Authors: Mucci Maria , Lenzi Francesca , D'Acunto Giulia Maria , Gazzillo Marisa , Accorinti Ilaria , Boldrini Silvia , Distefano Giacomo , Falcone Francesca , Fossati Beatrice , Giurdanella Annina Roberto , Paese Silvia , Salluce Carmen , Troiano Irene , Fratoni Cinzia , Fabiani Deborah , Liboni Francesca , Masi Gabriele
Published: December 2022

Dramatic events during the COVID-19 pandemic have acutely impacted the psychosocial environment worldwide, with negative implications for mental health, particularly for more vulnerable children and adolescents with severe psychiatric illnesses. Some data suggest that the pandemic waves may have produced different psychopathological consequences, further worsening in the second phase of the pandemic, compared to those in the first lockdown, soon after March 2020. To test the hypothesis of a further worsening of psychiatric consequences of COVID-19 in the second lockdown compared to the first lockdown, we focused our analysis on a consecutive sample of youth referred to a psychiatric emergency unit for acute mental disorders in the time period between March 2019–March 2021. The sample, consisting of 241 subjects (123 males and 118 females, ranging in age from 11 to 17 years), was divided into three groups: Pre-Lockdown Group (PLG, 115 patients); First Lockdown Group (FLG, 65 patients); and Second Lockdown Group (SLG, 61 patients). Patients in the SLG presented more frequently with non-suicidal self-injuries (NSSIs), suicidal ideation, and suicidal behavior, while no significant differences in self-harm were found between PLG and FLG. Eating disorders were more frequent in both the FLG and SLG, compared to the PLG, while sleep problems were higher only in the SLG. Furthermore, patients in the SLG presented with more frequent psychological maltreatments and neglect, as well as with psychiatric disorders in the parents. Adverse traumatic experiences and internalizing disorders were significantly associated with an increased risk of suicidality. Intellectual disability was less represented from the PLG to SLG, and similarly, the rate of ADHD was lower in the SLG. No differences were found for the other psychiatric diagnoses. This information may be helpful for a better understanding and management of adolescents with severe emotional and behavioral disorders after the exposure to long-lasting collective traumas.
Sudden Unexpected Postnatal Collapse and Therapeutic Hypothermia: What’s Going On?
Authors: Bedetti Luca , Lugli Licia , Garetti Elisabetta , Guidotti Isotta , Roversi Maria Federica , Della Casa Elisa , Miselli Francesca , Bariola Maria Carolina , Di Caprio Antonella , Pugliese Marisa , Ferrari Fabrizio , Berardi Alberto
Published: December 2022

Sudden unexpected postnatal collapse (SUPC) is a rare event, potentially associated with catastrophic consequences. Since the beginning of the 2000s, therapeutic hypothermia (TH) has been proposed as a treatment for asphyxiated neonates after SUPC. However, only a few studies have reported the outcome of SUPC after TH. The current study presents the long-term neurodevelopmental outcome of four cases of SUPC treated with TH in a single Italian center. Furthermore, we reviewed the previous literature concerning 49 cases of SUPC treated with TH. Among 53 total cases (of whom four occurred in our center), 15 (28.3%) died before discharge from the NICU. A neurodevelopmental follow-up was available only for 21 (55.3%) out of the 38 surviving cases, and seven infants developed neurodevelopmental sequelae. TH should be considered in neonates with asphyxia after SUPC. However, SUPC is a rare event, and there is a lack of comparative clinical data to establish the risk/benefit of TH after SUPC with different degrees of asphyxia. Analysis of large cohorts of newborns with SUPC, whether treated with TH or untreated, are needed in order to better identify infants who should undergo TH.
Systemic Lidocaine Infusions for Pediatric Patients with Cancer-Related Pain
Authors: Rav Emily , Sheth Rachna , Ahmad Ali H.
Published: December 2022

Pediatric patients with cancer experience significant distress from both treatment and cancer-related pain. Careful selection of an analgesic regimen should be based upon individual patient factors, including the level of pain, response to therapy, and physiologic profile. Refractory pain is a therapeutic dilemma frequently encountered in the pediatric cancer setting. Systemic lidocaine infusions have been described as both safe and efficacious, as prior studies show patients reporting decreased pain scores and improved quality of life after lidocaine treatment. Given the favorable side effect profile of lidocaine, it has the potential to be considered for analgesia in the setting of refractory pain. This review discusses the use of systemic lidocaine infusions for analgesia in pediatric oncology patients with cancer-related pain.
Predicting VO2max in Children and Adolescents Aged between 6 and 17 Using Physiological Characteristics and Participation in Sport Activities: A Cross-Sectional Study Comparing Different Regression Models Stratified by Gender
Authors: Carayanni Vilelmine , Bogdanis Gregory C. , Vlachopapadopoulou Elpis , Koutsouki Dimitra , Manios Yannis , Karachaliou Feneli , Psaltopoulou Theodora , Michalacos Stefanos
Published: December 2022

Background: The aim of this study is to use different regression models to capture the association between cardiorespiratory fitness VO2max (measured in mL/kg/min) and somatometric characteristics and sports activities and making better predictions. Methods: multiple linear regression (MLR), quantile regression (QR), ridge regression (RR), support vector regression (SVR) with three different kernels, artificial neural networks (ANNs), and boosted regression trees (RTs) were compared to explain and predict VO2max and to choose the best performance model. The sample consisted of 4908 children (2314 males and 2594 females) aged between 6 and 17. Cardiorespiratory fitness was assessed by the 20 m maximal multistage shuttle run test and maximal oxygen uptake (VO2max) was calculated. Welch t-tests, Mann–Whitney-U tests, X2 tests, and ANOVA tests were performed. The performance measures were root mean square error (RMSE), mean absolute error (MAE), and coefficient of determination (R2). All analyses were stratified by gender. Results: A comparison of the statistical indices for both the predicted and actual data indicated that in boys, the MLR model outperformed all other models in all indices, followed by the linear SVR model. In girls, the MLR model performed better than the other models in R2 but was outperformed by SVR-RBF in terms of RMSE and MAE. The overweight and obesity categories in both sexes (p < 0.001) and maternal prepregnancy obesity in girls had a significant negative effect on VO2max. Age, weekly football training, track and field, basketball, and swimming had different positive effects based on gender. Conclusion: The MLR model showed remarkable performance against all other models and was competitive with the SVR models. In addition, this study’s data showed that changes in cardiorespiratory fitness were dependent, to a different extent based on gender, on BMI category, weight, height, age, and participation in some organized sports activities. Predictors that are not considered modifiable, such as gender, can be used to guide targeted interventions and policies.
Baicalein Relieves Ferroptosis-Mediated Phagocytosis Inhibition of Macrophages in Ovarian Endometriosis
Authors: Yi Zhi-Hui , Li Shu-Qing , Ke Jun-Ya , Wang Yun , Zhao Ming-Zhi , Li Jing , Li Ming-Qing , Zhu Zhi-Ling
Published: December 2022

Iron overload and oxidative stress have been reported to contribute to ferroptosis in endometriotic lesions. However, the possible roles of iron overload on macrophages in endometriosis (EMs) remain unknown. Based on recent reports by single-cell sequencing data of endometriosis, here we found significant upregulations of ferroptosis-associated genes in the macrophage of the endometriotic lesion. Additionally, there was an elevated expression of HMOX1, FTH1, and FTL in macrophages of peritoneal fluid in EMs, as well as iron accumulation in the endometriotic lesions. Notably, cyst fluid significantly up-regulated levels of intracellular iron and ferroptosis in Phorbol-12-myristate-13-acetate (PMA)-stimulated THP-1 cells. Additionally, high iron-induced ferroptosis obviously reduced PMA-stimulated THP-1 cells’ phagocytosis and increased the expression of angiogenic cytokines, such as vascular endothelial growth factor A (VEGFA) and interleukin 8 (IL8). Baicalein, a potential anti-ferroptosis compound, increased GPX4 expression, significantly inhibited ferroptosis, and restored phagocytosis of THP-1 cells in vitro. Collectively, our study reveals that ferroptosis triggered by high iron in cyst fluid promotes the development of EMs by impairing macrophage phagocytosis and producing more angiogenic cytokines (e.g., IL8 and VEGFA). Baicalein displays the potential for the treatment of EMs, especially in patients with high ferroptosis and low phagocytosis of macrophages.
Stabilization of Sandy Soils by Bentonite Clay Slurry at Laboratory Bench and Pilot Scales
Authors: Bani Baker Mousa , Abendeh Raed , Sharo Abdulla , Hanna Adel
Published: December 2022

Sand is one of the most abundant, naturally occurring materials in many parts of the world, which is used in local rural areas in infrastructure projects such as in the construction of low volume paved and unpaved road layers due to their availability at low cost and scarcity of other suitable construction materials. Several geotechnical solutions for sand stabilization have been undertaken to improve their properties in order to overcome erosion, failure of pavements under traffic loading, embankments, cuts and excavations caused by failures of sand structure. In this investigation, bentonite clay–water slurry was used due to its cohesive and eco-friendly nature to improve sand strength by the means of manual injection in the laboratory and pilot scales. Sand was stabilized using variation of bentonite clay contents, 0%, 1%, 2%, 3%, and 4% (by weight of dry sand), at different curing times: 0 days, 1 day, 2 days, and 3 days. Direct shear tests were conducted to determine shear strength parameters for sand before and after stabilization process. Furthermore, a transparent polypropylene box (60 cm × 40 cm × 30 cm) was used in this study as a larger scale for sand stabilization technique by applying manual grouting of bentonite clay–water slurry to the sand mass. A mechanical shaker was used at 100, 200, 300, and 400 rpm for 10 min at each stage to test the stability of sand in addition to using a Scanning Electron Microscope (SEM) to obtain images for stabilized sand and Ground Penetrating Radar (GPR) to scan soil mass before and after stabilization. The test results showed that a slurry composed of 3% of bentonite clay additive with 10.3% added water by weight of dry sand mass are the optimum amounts for the stabilization process, which provides a substantial resistance to shear forces.
A Novel Simple In Vitro System Mimicking Natural Environment for the Biofilm Cultivation of Cutaneous Bacteria
Authors: Mart’yanov Sergey , Gannesen Andrei , Plakunov Vladimir
Published: December 2022

Biofilms are microbial communities of cells embedded in extracellular matrix, and they are regarded as a major form of the natural and laboratory occurrence of bacteria. Cutaneous microbiota is represented by prokaryotic and eukaryotic organisms, which form biofilms in the different niches including the skin surface, glands, and hair follicles. Despite of a large number of in vitro studies dedicated to the biofilms of cutaneous bacteria, the methods used usually do not closely take into account the specific surroundings of certain skin parts. In this study, we introduce a new simple method of biofilm cultivation on the solid keratine/agarose pellets embedded in polyacrylamide gel. In such a model system, we tried to minimize the amount of liquid phase, which makes a model close to a human comedo, and provide a prominent biofilm formation of selected cutaneous bacteria.
Coatings in Industry Research Methods—A Short Review
Authors: Borawski Andrzej
Published: December 2022

Coatings of various types are very willingly and widely used in all industries [...]
Effect of Heat Sealing and Storage on Mechanical and Barrier Properties of Maillard Modified Fish Skin Gelatin/L-arabinose Composite Films
Authors: Zheng Qiankun , Zhang Qiang , Chen Fusheng , Yin Lijun
Published: December 2022

The increasing accumulation of non-degradable plastic food packaging is a global concern. In this study, we aimed to optimize the heat-sealing process of fish skin gelatin/L-arabinose (FG-Ara) composite films modified by the Maillard reaction. The effects of storage temperature, humidity, and time on the mechanical and barrier properties of the modified FG-Ara composite films were investigated. The optimal heat-sealing parameters were 24 V, with vacuum, heat-sealing, and cooling times of 7.0, 3.0, and 3.0 s, respectively. After 120 days of storage at low temperatures (4, −17 °C), the tensile strength of the composite films decreased to 11.15 ± 1.64 and 13.14 ± 1.68 MPa, respectively, and the elongation at break increased by 39.07% and 18.29% compared with the initial value, respectively. Moreover, the water vapor permeability in the low-temperature treatment groups remained relatively stable at the initial stages of storage (0–40 days) and reached 39.57 ± 3.09 and 26.95 ± 18.50 g·mm/m2·d·kPa after 120 days, respectively. The milk powder packed in the FG-Ara composite films had good quality and no hardening in low-temperature environments. After 120 days of storage, the peroxide value of the soybean oil packed in the films still met the quality standard of first-grade soybean oil. Furthermore, the Arrhenius equation was used to fit the peroxide values of soybean oil at different temperatures. The maximum shelf life of soybean oil coated by the composite film was predicted to be 250 days. The experimental results demonstrated that the optimized films might serve as effective food packaging materials.
The Effect of Multi-Walled Carbon Nanotubes on the Compressive Strength of Cement Mortars
Authors: Muradyan Nelli G. , Gyulasaryan Harutyun , Arzumanyan Avetik A. , Badalyan Maria M. , Kalantaryan Marine A. , Vardanyan Yeghiazar V. , Laroze David , Manukyan Aram , Barseghyan Manuk G.
Published: December 2022

In this work, multi-walled carbon nanotubes (MWCNTs) have been synthesized using a modified method of solid-phase pyrolysis. The MWCNTs are effectively dispersed using a simple and facile method such as ultrasonic energy without and with surfactant for two different sonication times (15 min and 40 min). In the present study, the effect of MWCNT concentration (0.001, 0.01, 0.05, 0.1 wt.%) on the compressive strengths of cement mortars has been investigated. Compressive tests were carried out on an automatic pressure machine (C089) with a loading rate of 0.5 kN/s at the age of 7 days and 28 days. It is shown that the optimal value of the nanotubes’ concentration does not exist in the case of 15 min of sonication time, whereas the optimal value for 40 min of sonication time without and with surfactant is 0.01%. Moreover, in the absence of surfactants, the strength of the specimen over 7 days of hardening increased by 13%, and by 19.5% in the presence of surfactants. The compressive strength for a curing period of 28 days increased by 6.3% and 13.8%, respectively.
Electrochemical Behavior of Nickel Aluminide Coatings Produced by CAFSY Method in Aqueous NaCl Solution
Authors: Marinou Amalia , Lekatou Angeliki G. , Xanthopoulou Galina , Vekinis George
Published: December 2022

Combustion-assisted flame spraying (CAFSY) is a novel method that allows in-flight synthesis of alloys during flame spraying. The in-flight synthesis of alloys by the CAFSY method during flame spraying combines two different methods: the self-propagating high-temperature synthesis (SHS) and flame spraying (FS). The present work studies the corrosion performance (by cyclic polarization and chronoamperometry in aerated 3.5 wt.% NaCl) of NiAl coatings fabricated by the CAFSY technique in relation to main process parameters (composition of the initial feedstock, spraying distance, substrate temperature, postdeposition heat treatment) and their effect on the microstructure and porosity of the coatings. Most of the coatings exhibited limited susceptibility to localized corrosion. In all cases, the steel substrate remained intact despite corrosion. Interconnected porosity was the main parameter accelerating uniform corrosion. Localized corrosion had the form of pitting and/or crevice corrosion in the coating that propagated dissolving Al and Al-rich nickel aluminides along coating defects. Substrate preheating and postdeposition heat treatment negatively affected the corrosion resistance. A short spraying distance (1.5 inch) increased the corrosion resistance of the coatings.
Chemical Agglomeration to Enhance Blast Furnace Dust Capture Efficiency in Wet Electrostatic Precipitators
Authors: Han Yingying , Xiao Lichun , Chen Hongrui
Published: December 2022

Wet electrostatic precipitators (WESPs) are increasingly used in iron and steel plants and coal-fired power plants due to their higher efficiency at capturing fine particles than conventional dry-type precipitators. In order to achieve ultra-pure purification of dust in steel plant gas, we propose an improved method that involves adding a chemical coagulant and a surfactant to a WESP. The effects of the type and concentration of chemical coagulant and surfactant on the agglomeration effect and dust removal efficiency of blast furnace dust were investigated. The results show that the addition of a chemical coagulant could promote the agglomeration of blast furnace dust particles, and the D50 of dust particles increased from 5.8 to 15.0 μm after the addition of xanthan gum (XTG). The best increase in the blast furnace’s dust particle removal occurred at a concentration of 10 mg/L of XTG, and the dust removal efficiency reached 97.59%. The surfactant dodecyl trimethyl ammonium chloride (DTAC) improved the dust removal efficiency of blast furnace dust when added alone. The dust removal efficiency reached 97.82% when 10 mg/L of XTG and 9 mg/L of DTAC were added synergistically. The addition of a chemical coagulant and surfactant promoted the agglomeration of blast furnace dust and enhanced the dust capture effect of a WESP. We thus demonstrated that we can improve the efficiency of WESP in the future via chemical coagulation. The authors will further study the effect of multi-factor synergistic coupling on the chemical coagulation method in WESPs.