bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in the Left Ventricular Eicosanoid Profile in Human Dilated Cardiomyopathy
Authors: Sosnowski Deanna K. , Jamieson K. Lockhart , Darwesh Ahmed M. , Zhang Hao , Keshavarz-Bahaghighat Hedieh , Valencia Robert , Viveiros Anissa , Edin Matthew L. , Zeldin Darryl C. , Oudit Gavin Y. , Seubert John M.
Published: May 2022

Objective

Metabolites derived from N−3 and N−6 polyunsaturated fatty acids (PUFAs) have both beneficial and detrimental effects on the heart. However, contribution of these lipid mediators to dilated cardiomyopathy (DCM)-associated mitochondrial dysfunction remains unknown. This study aimed to characterize DCM-specific alterations in the PUFA metabolome in conjunction with cardiac mitochondrial quality in human explanted heart tissues.

Methods

Left ventricular tissues obtained from non-failing control (NFC) or DCM explanted hearts, were assessed for N−3 and N−6 PUFA metabolite levels using LC-MS/MS. mRNA and protein expression of CYP2J2, CYP2C8 and epoxide hydrolase enzymes involved in N−3 and N−6 PUFA metabolism were quantified. Cardiac mitochondrial quality was assessed by transmission electron microscopy, measurement of respiratory chain complex activities and oxygen consumption (respiratory control ratio, RCR) during ADP-stimulated ATP production.

Results

Formation of cardioprotective CYP-derived lipid mediators, epoxy fatty acids (EpFAs), and their corresponding diols were enhanced in DCM hearts. These findings were corroborated by increased expression of CYP2J2 and CYP2C8 enzymes, as well as microsomal and soluble epoxide hydrolase enzymes, suggesting enhanced metabolic flux and EpFA substrate turnover. DCM hearts demonstrated marked damage to mitochondrial ultrastructure and attenuated mitochondrial function. Incubation of fresh DCM cardiac fibers with the protective EpFA, 19,20-EDP, significantly improved mitochondrial function.

Conclusions

The current study demonstrates that increased expressions of CYP-epoxygenase enzymes and epoxide hydrolases in the DCM heart correspond with enhanced PUFA-derived EpFA turnover. This is accompanied by severe mitochondrial functional impairment which can be rescued by the administration of exogenous EpFAs.
Perioperative and Short-Term Outcomes of Sinus Replacement and Conservative Repair for Aortic Root in Acute Type A Aortic Dissection: A Prospective Cohort Study
Authors: Chang Yi , Qian Xiangyang , Guo Hongwei , Wei Yizhen , Yu Cuntao , Sun Xiaogang , Wei Bo , Ma Qiong , Shi Yi
Published: May 2022

Background

To compare outcomes between sinus replacement (SR) and conservative repair (CR) for dissected roots with normal size.

Methods

From October 2018 to April 2021, a prospective cohort study was carried out. Patients were assigned to two groups (SR group and CR group) according to whether they underwent sinus replacement. Propensity score matching was applied to adjust preoperative variables and Kaplan–Meier method was used for survival analysis.

Results

Three hundred and eighty-seven patients were enrolled. In the whole cohort, 18 patients (4.7%) died postoperatively. The operative mortality of SR group was comparable to CR group (3.2% vs. 6.0%, p = 0.192 before matching; 3.5% vs. 7.0%, p = 0.267 after matching) and the incidence of hemostasis management under restarted cardiopulmonary bypass for root bleeding was lower in SR group (1.6% vs. 7.0%, p = 0.002 before matching; 2.1% vs. 8.5%, p = 0.03 after matching). The median follow-up duration was 12 months. There were 3 reoperations in the CR group. The estimated cumulative event rate of reoperation was 1.1 % at 12 months and 1.6% at 24 months in CR group, with a trend of a lower rate in the SR group (log-rank p = 0.089 before matching, p = 0.075 after matching). There was one late death in each group. The estimated cumulative event rate of death was 3.8% at 12 months and 24 months in the SR group, and was 6.6% in the CR group with no significant difference (log-rank p = 0.218 before matching, p = 0.120 after matching). Aortic regurgitation significantly improved postoperatively and remained stable during follow-up.

Conclusions

Sinus replacement is a simple, safe, and effective technique for repairing severely dissected sinus with a comparable time spent in operation and excellent immediate and short-term results. It had the advantages of eliminating false lumen and avoiding aortic root bleeding.
Elastin Insufficiency Confers Proximal and Distal Pulmonary Vasculopathy in Mice, Partially Remedied by the KATP Channel Opener Minoxidil: Considerations and Cautions for the Treatment of People With Williams-Beuren Syndrome
Authors: Knutsen Russell H. , Gober Leah M. , Kronquist Elise K. , Kaur Maninder , Donahue Danielle R. , Springer Danielle , Yu Zu Xi , Chen Marcus Y. , Fu Yi-Ping , Choobdar Feri , Nguyen My-Le , Osgood Sharon , Freeman Joy L. , Raja Neelam , Levin Mark D. , Kozel Beth A.
Published: May 2022

Background

Williams Beuren syndrome (WBS) is a recurrent microdeletion disorder that removes one copy of elastin (ELN), resulting in large artery vasculopathy. Early stenosis of the pulmonary vascular tree is common, but few data are available on longer-term implications of the condition.

Methods

Computed tomography (CT) angiogram (n = 11) and echocardiogram (n = 20) were performed in children with WBS aged 3.4–17.8 years. Controls (n = 11, aged 4.4–16.8 years) also underwent echocardiogram. Eln+/− mice were analyzed by invasive catheter, echocardiogram, micro-CT (μCT), histology, and pressure myography. We subsequently tested whether minoxidil resulted in improved pulmonary vascular endpoints.

Results

WBS participants with a history of main or branch pulmonary artery (PA) stenosis requiring intervention continued to exhibit increased right ventricular systolic pressure (RVSP, echocardiogram) relative to their peers without intervention (p < 0.01), with no clear difference in PA size. Untreated Eln+/− mice also show elevated RVSP by invasive catheterization (p < 0.0001), increased normalized right heart mass (p < 0.01) and reduced caliber branch PAs by pressure myography (p < 0.0001). Eln+/− main PA medias are thickened histologically relative to Eln+/+ (p < 0.0001). Most Eln+/− phenotypes are shared by both sexes, but PA medial thickness is substantially greater in Eln+/− males (p < 0.001). Eln+/− mice showed more acute proximal branching angles (p < 0.0001) and longer vascular segment lengths (p < 0.0001) (μCT), with genotype differences emerging by P7. Diminished PA acceleration time (p < 0.001) and systolic notching (p < 0.0001) were also observed in Eln+/− echocardiography. Vascular casting plus μCT revealed longer generation-specific PA arcade length (p < 0.0001), with increased PA branching detectable by P90 (p < 0.0001). Post-weaning minoxidil decreased RVSP (p < 0.01) and normalized PA caliber (p < 0.0001) but not early-onset proximal branching angle or segment length, nor later-developing peripheral branch number.

Conclusions

Vascular deficiencies beyond arterial caliber persist in individuals with WBS who have undergone PA stenosis intervention. Evaluation of Eln+/− mice reveals complex vascular changes that affect the proximal and distal vasculatures. Minoxidil, given post-weaning, decreases RVSP and improves lumen diameter, but does not alter other earlier-onset vascular patterns. Our data suggest additional therapies including minoxidil could be a useful adjunct to surgical therapy, and future trials should be considered.
Development of a Consensus-Based Cross-Domain Protocol for the Management of Elastic Compression Stocking Therapy in Patients With Deep Venous Thrombosis and Chronic Venous Disease: A Modified Delphi Study
Authors: Schreurs Rachel H. P. , Joore Manuela A. , ten Cate Hugo , ten Cate-Hoek Arina J.
Published: May 2022

Objective

Elastic compression stocking (ECS) therapy is commonly used in patients with deep venous thrombosis (DVT) and chronic venous disease (CVD). The provision of ECS therapy is complex, and studies indicate a lack of practical guidance and suboptimal collaboration among health care professionals. We aimed to reach consensus on critical issues of ECS therapy among the involved health care professionals and patients.

Methods

A three-round modified Delphi analysis was performed in the Netherlands in which 56 health care professionals (internists, dermatologists, general practitioners, emergency room nurses, home care nurses, medical stocking suppliers, and occupational therapists) and seven patients were invited. The 21 statements included in this analysis were based on information collected from a previously conducted Functional Resonance Analysis Method and Realist Evaluation. We used 7-point Likert scale questions and a 75% threshold for consensus.

Results

Of the 63 persons invited for this study, 59 (94%) agreed to participate and responded in the first questionnaire round; of whom 52 were health care professionals and seven were patients (five DVT and two CVD). The overall response rate for the three questionnaire rounds was 91%. After completion of the rounds, full consensus was achieved on 19 out of 21 statements. No consensus was reached on the need for a follow-up appointment for CVD patients and who should be responsible to determine the ECS type (custom-made or standard).

Conclusion

We identified 19 consensus-driven recommendations on treatment decisions and collaboration in ECS therapy among an interdisciplinary panel of health care professionals and patients. These recommendations form a basis for consensus-driven optimization of ECS therapy and should ideally be incorporated in a general cross-domain protocol for ECS therapy in patients with DVT and CVD.
Physical Exercise in Resistant Hypertension: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Saco-Ledo Gonzalo , Valenzuela Pedro L. , Ruilope Luis M. , Lucia Alejandro
Published: May 2022

Physical exercise reduces blood pressure (BP) in patients with hypertension in general but more evidence is needed specifically for a high-risk phenotype associated with intensive medication, resistant hypertension (RH). In this systematic review and meta-analysis, we aimed to summarize current evidence of the exercise effects on BP in patients with RH. A systematic search was conducted in PubMed, Web of Science and Cochrane Library (from inception to 3rd November, 2021). A random effects meta-analysis was performed when at least two trials assessed the effect of either acute or regular exercise (vs. a control condition) on the same outcome. Ten studies (N = 380 participants; 51% female; mean age 52 to 67 years) were included in the review, of which four (N = 58) and six (N = 322) assessed the effects of acute and regular exercise, respectively. Evidence overall suggests that a single bout of acute exercise results in a short-term (≤ 24 h) reduction of BP, although no meta-analysis could be performed. As for regular exercise, three randomized controlled trials (N = 144, 50% female) could be meta-analyzed, which showed that exercise training intervention (8–12 weeks, 3 sessions/week) significantly reduces 24-h (−9.9 mmHg, 95% confidence interval −15.4−4.4 for systolic BP; and −5 mmHg, −7.0−3.0 for diastolic BP) and daytime ambulatory BP (−11.7 mmHg, −17.8−5.7; and −7.4 mmHg, −11.9−2.9). In summary, physical exercise appears as an effective option to reduce BP in patients with RH, although more research is needed to confirm these findings as well as to determine the most effective exercise characteristics.
Brown Adipose Tissue and Novel Management Strategies for Polycystic Ovary Syndrome Therapy
Authors: Zhang Qiaoli , Ye Rongcai , Zhang Yuan-Yuan , Fan Chen-Chen , Wang Jun , Wang Shuyu , Chen Suwen , Liu Xiaowei
Published: May 2022

Brown adipose tissue (BAT), a unique tissue, plays a key role in metabolism and energy expenditure through adaptive nonshivering thermogenesis. It has recently become a therapeutic target in the treatment of obesity and metabolic diseases. The thermogenic effect of BAT occurs through uncoupling protein-1 by uncoupling adenosine triphosphate (ATP) synthesis from energy substrate oxidation. The review discusses the recent developments and progress associated with the biology, function, and activation of BAT, with a focus on its therapeutic potential for the treatment of polycystic ovary syndrome (PCOS). The endocrine activity of brown adipocytes affects the energy balance and homeostasis of glucose and lipids, thereby affecting the association of BAT activity and the metabolic profile. PCOS is a complex reproductive and metabolic disorder of reproductive-age women. Functional abnormalities of adipose tissue (AT) have been reported in patients with PCOS. Numerous studies have shown that BAT could regulate the features of PCOS and that increases in BAT mass or activity were effective in the treatment of PCOS through approaches including cold stimulation, BAT transplantation and compound activation in various animal models. Therefore, BAT may be used as a novel management strategy for the patients with PCOS to improve women’s health clinically. It is highly important to identify key brown adipokines for the discovery and development of novel candidates to establish an efficacious therapeutic strategy for patients with PCOS in the future.
An Adaptive Voltage Control Using Local Voltage Profile Mode and Similarity Ranking
Authors: Ma Haomin , Wang Genping , Gao Xiang , Zou Zhouyiao , Dong Yanfei
Published: May 2022

Emergency voltage control provides a real-time online response to maintain the long-term voltage stability of a power system. Searching for an optimal emergency control solution is a hard combinatorial optimization problem because of the highly dynamic and nonlinear characteristics of power systems with discrete control variables. Additionally, real-time response is required. A new mode-matching–based adaptive voltage control is proposed in this article to achieve a fast response by exploring local voltage profile modes and the technique of similarity ranking. First, some typical emergencies are studied in advance, and their control knowledge is collected for preparation. The set of local voltage profile modes and their corresponding optimal control solutions are stored as knowledge for each emergency. Second, fast online control is realized and the control solution is configured according to a similarity ranking among local voltage profile modes. Furthermore, a learning process is applied to accumulate knowledge in case of unprepared situations. Thus, improved control performances in the future are ensured. This article depends on a new concept of local voltage profile mode with which knowledge preparation, emergency identification, configuration of control, and knowledge accumulation work together to perform an effective response. Simulations of the IEEE 39-bus system verify the outcomes of this voltage control scheme.
RETRACTED: Assisting Smart Construction With Reliable Edge Computing Technology
Authors: Yue Qiang , Mu Song , Zhang Longguan , Wang Zhun , Zhang Zhonghua , Zhang Xing , Wang Yongge , Miao Zhuang
Published: May 2022

Although smart construction tools are already assisting the construction sector, still major reforms are considerably seen in the coming years with Artificial Intelligence of Things (AIoT). The purpose of this work was to develop a down-to-earth reliable edge-computing design and plan, which can be utilized to bolster savvy development situations with high quality of service. The number of devices connected to industrial processes is growing in tandem with the advancement of sophisticated technologies and the use of the AIoT. Big data are created as gadgets grow more integrated and need more computing power. However, as the amount of big data generated grows, processing and analytical issues arise. Cloud computing technologies are now being used to solve processing and analytical challenges. However, by using edge computing technologies, greater emphasis is placed on executing calculations as near to the device as feasible. As a result of these facts, this article suggests the arrangement plan, which depends on the most recent cloud and computer program designing approaches and advances, and gives flexibility, interoperability, and adjustment to construction companies' particular needs.
The Water, Energy, and Food Nexus: Health is yet Another Resource
Authors: Nuwayhid Iman , Mohtar Rabi
Published: May 2022

This perspective highlights a place for Health (H) in the Water, Energy, and Water (WEF) nexus. It reviews the reference to health in the WEF nexus literature and makes the case for its inclusion into the WEF Nexus. We argue that although the nexus concept of water, energy, and food is relatively recent, it has been adopted by several UN agencies and international organizations and it will continue to draw emphasis in research, politics and communications of the scientific community. Now is the time to integrate health.
How to Improve Farmers’ Green Production Level in a Targeted Manner?
Authors: Yu YanLi , Lu Ting , Hu Ya Guan , Meng KaiWen , Li Hua
Published: May 2022

Improving farmers’ green production behavior can guarantee food safety at the source. In recent years, a rising number of studies have focused on food safety management and have provided general regulation recommendations. Unlike many studies, this study aimed to find targeted policy recommendations according to different spatial aggregations of non-green production behavior. In the current study, more than 800 tea farmers located in the Qinba and Huangshan Mountain regions of China were investigated. An order logit model was employed to evaluate the impact of government regulation and community governance on ignorant or unkind non-green tea production behavior. Furthermore, a multi-valued treatment effect model was also recruited to demonstrate the average treatment effect of government regulation and community governance. The results show that the difference in farmers’ ignorant or unkind non-green production behavior between regions is substantial but is similar in the same region. Farmers’ non-green production behavior is negatively impacted by community governance but not significantly affected by government regulation. Government regulation can effectively inhibit the occurrence of farmers’ unkind behavior, while community governance can effectively cause farmers’ unkind behavior. Thus, some measures to reduce local farmers’ non-green production behavior should be put forward according to the differences in the spatial distribution of non-green production behavior and the differences in the effect of government regulation and community governance.
Can Industrial Relocation Reduce Air Pollution? Evidence From a Quasi-Natural Experiment in China
Authors: Ge Tao , Lv Xuchen , Ma Li , Shen Xiaoyan
Published: May 2022

To reduce the increasingly severe air pollution caused by rapid industrialization, China has introduced a National Industrial Relocation Demonstration Zones (NIRDZs) policy. This paper takes the NIRDZs as a quasi-natural experiment and employs the difference-in-differences (DID) method to test the effects of industrial relocation on air pollution based on panel data of 285 prefecture-level cities from 2003 to 2018. Results show that the NIRDZs have an inhibitory effect on SO2 emissions, although their local effect is significant in the first 5 years and their spillover effect only occurs within 50–100 km. Mechanism analysis reveals that the NIRDZs reduce air emissions by rationalizing and upgrading the industrial structure. Additionally, further discussions suggest that cities with moderate administrative areas and abundant natural resources should be prioritized as pilot cities, and industries including nonferrous metals, steel, automotive, new energy, new materials, and producer services should be designated as priority industries.
Do Freshwater Turtles Use Rainfall to Increase Nest Success?
Authors: Geller Gregory A. , Doody J. Sean , Clulow Simon , Duncan Richard P.
Published: May 2022

Rainfall following turtle nest construction has long been believed to increase nest survival by its effects on reducing the location cues used by nest predators. However, it is unclear if this is generally the case and if nesting turtles actively use this mechanism to increase their reproductive fitness by deliberately timing nesting to occur before or during rainfall. To address this question, we reviewed studies that examined freshwater turtle nesting behavior and nest predation rates in relation to rainfall. We supplemented our review with data on rainfall and nesting patterns from a 12-year study of two nesting populations of Ouachita Map Turtles (Graptemys ouachitensis). Our review revealed a diversity of responses in rainfall effects on predation and in the propensity for turtles to nest in association with rain. Our mixed findings could reflect a diversity of species- or population-specific responses, local adaptations, species composition of predator community, confounding abiotic factors (e.g., temperature decreases after rainfall) or methodology (e.g., most studies did not quantify rainfall amounts). Our case study on map turtles found very high yearly predation rates (75–100%), precluding our ability to rigorously analyze the association between nest predation and rainfall. However, close examination of the exact timing of both rainfall and predation revealed significantly lower predation rates when rain fell within 24 h after nesting, indicating that rainfall during or after nesting may reduce nest predation. Despite this effect, the best fitted model explaining the propensity to nest found that map turtles were more likely to nest after dry days than after days with rainfall, suggesting that rainfall was not a major factor driving turtles to nest in our populations. In both our review and in our map turtle populations there was little evidence that turtles can anticipate rainfall and nest prior to it occurring (e.g., in response to falling barometric pressure).
Development of Therapeutic RNA Manipulation for Muscular Dystrophy
Authors: Saifullah  , Motohashi Norio , Tsukahara Toshifumi , Aoki Yoshitsugu
Published: May 2022

Graphical Abstract
Pharmacokinetics and Genetic Factors of Atorvastatin in Healthy Korean Subjects
Authors: Kim Serim , Seo Jong Do , Yun Yeo-Min , Kim Hanah , Kim Tae-Eun , Lee Taeheon , Lee Tae-Rim , Lee Jun Hyung , Cho Eun-Hae , Ki Chang-Seok
Published: May 2022

Background: Statins are the most popular agents for the primary and secondary prevention of cardiovascular disease; however, the pharmacokinetic parameters and associated genetic factors in the Korean population have not been fully elucidated. This study explored the pharmacokinetic properties of atorvastatin and the association between genetic variations and atorvastatin pharmacokinetics in healthy Korean subjects.

Methods: Atorvastatin (80 mg) was administered to 35 healthy Korean volunteers. Plasma levels of atorvastatin and its metabolites were measured sequentially using liquid chromatography-tandem mass spectrometry from 0 to 24 h after atorvastatin administration. Customized next-generation sequencing analysis was performed covering all coding exons of 15 genes, as well as 46 single-nucleotide variants in 29 genes related to statin pharmacokinetics.

Results: The mean area under the concentration-time (AUC) and Cmax (maximum peak concentration) were 269.0 ng/ml∙h and 84.3 ng/ml, respectively, which were approximately two times higher than those reported in Caucasians. Genetic analysis revealed that eight genetic variants in ABCB1, ABCG2, APOA5, CETP, and CYP7A1 contributed to the AUC of atorvastatin. The atorvastatin AUC0–24 h prediction model was developed based on age and eight genetic variants using multivariate linear regression (adjusted R2 = 0.878, p < 0.0001).

Conclusion: This study shows that the pharmacokinetic properties of atorvastatin in Koreans are different from those in Caucasians and that atorvastatin AUC0–24 h could be predicted based on age and eight genetic variants of ABCB1, ABCG2, APOA5, CETP, and CYP7A1.
Implication of Macroalgal Bloom to Soil Organic Carbon Stock in Seagrass Meadows - A Case Study in South Hainan, China
Authors: Chen Shunyang , Chen Shiquan , Chen Bin , Wu Zhongjie , An Wenshuo , Luo Lizhen , Wang Jing , Xie Limei , Zhang Jing , Chen Guangcheng
Published: May 2022

The adverse impact of macroalgal blooms associated with nutrient enrichment on seagrass habitats and carbon storage potential is an ecological concern. In the present study, the soil carbon stock and sources were compared between a seagrass meadow where a serious macroalgal bloom occurred (site M) and an adjacent site without apparent macroalgae (site R) in a nutrient-enriched lagoon in South Hainan Island, China, to test whether macroalgal blooms associated with nutrient enrichment would impact the soil carbon in seagrass meadows. The soil organic carbon (OC) and total nitrogen contents in the top 30 cm at site M were significantly lower than those at site R. The soil OC stocks (top 30 cm) were 3.4 and 5.4 Mg C ha-1 at site M and site R, respectively, and no difference was observed between sampling stations with different distances offshore at either site. Soil δ13C was more enriched and closer to the δ13C of seagrass tissues at site R than at site M. Bayesian stable isotope mixing model analyses suggested that seagrass-derived material contributed ~50% to soil OC at site R, while at site M, the contribution was reduced to ~25%. The results suggested that macroalgal blooms associated with nutrient enrichment could drive the loss of seagrass-derived OC and the OC stock in the soil, which is worthy of full attention for blue carbon conservation.
Numerical and Experimental Study on Reasonable Coverage of Shot Peening on ZGMn13 High Manganese Steel
Authors: Yuan Haiyang , You Zhangping , Zhuo Yaobin , Ye Xiaoping , Zhu Liangliang , Yang Weibo
Published: May 2022

Shot peening technology is usually employed to improve the ability of mechanical parts to resist failure due to fatigue and wear. It is often used to strengthen the surface of a target, but the induced residual stress and its distribution with respect to the coverage can affect the performance of the shot peening process. In this study, a comprehensive numerical and experimental study was conducted to overcome these issues. Using numerical simulation we found that both the surface and subsurface residual stress increases with the increase of the coverage before stabilizing. Quantitative analysis using the Entropy Method indicates that under the shot peening parameters considered in the simulation coverage of 200% is best for the shot peening of ZGMn13 High Manganese Steel. The following experimental study agreed with the corresponding numerical data for the residual stresses at varied depths from surface to subsurface with errors of less than 25%. Thus, the related research outcomes can guide the shot peening process to obtain the optimized surface strengthening of the target.
Biometric and Structural Ocular Manifestations of Anterior Megalophthalmos
Authors: Chen Tian-Hui , Chen Ze-Xu , Zhang Min , Chen Jia-Hui , Lan Li-Na , Jiang Yongxiang
Published: May 2022

Objective

The aim of this study was to examine the biometric ocular manifestations and structural ocular features of anterior megalophthalmos (AM).

Methods

Fifteen patients with AM (30 eyes) from the Eye & ENT Hospital of Fudan University were included. The age-matched control group consisted of 30 participants (30 eyes) who underwent Pentacam HR and IOLMaster 700 measurements for one normal eye. Data on demographics, biometric manifestations, and genotypes were carefully compared.

Results

A total of 15 patients with AM and 30 control patients were enrolled. There were no differences in age (37.27 ± 19.1 vs. 31.43 ± 19.69 years, P = 0.249) between these two groups. AM eyes were characterized by premature cataracts (11/30, 36.67%) and zonular weakness with lens subluxation (22/30, 73.33%) compared with the control group. Notably, 20 of the 30 AM eyes (66.67%) had significant posterior iris bowing, and 16 of the 30 AM eyes (53.33%) showed an enlarged ciliary ring on ultrasound biomicroscopy (UBM). Mean corneal curvature was lower in the AM eyes (42.01 ± 2.06 D vs. 43.14 ± 1.38 D, P = 0.023). There was no significant difference in corneal pachymetry and central endothelial cell count between the AM and control groups. Significant differences were found in terms of the anterior chamber and white-to-white (WTW) among the Pentacam HR and IOLMaster 700 in patients with AM (P < 0.05). The difference was 0.53 ± 0.48 mm and 0.36 ± 0.14 mm, respectively (P < 0.001).

Conclusion

The results of this cohort study conclude the biometric and structural ocular manifestations in Chinese cohorts. Posterior iris bowing (66.67%) and lens subluxation (73.33%) are the most characteristic findings in patients with AM with anatomical abnormalities of megalocornea and a deep anterior chamber, although corneal biometric manifestations of AM included flatter cornea and lower total corneal astigmatism. The knowledge of ocular manifestations of AM is important for diagnosis and preparation for the operation in advance to avoid intraoperative and postoperative complications. Significant differences were found in the anterior chamber and WTW values between the Pentacam HR and IOLMaster 700. Thus, we suggest that various examinations should be carefully considered before determining an AM diagnosis.
Differentiating Phenotypes of Coronavirus Disease-2019 Pneumonia by Electric Impedance Tomography
Authors: Lovas András , Chen Rongqing , Molnár Tamás , Benyó Balázs , Szlávecz Ákos , Hawchar Fatime , Krüger-Ziolek Sabine , Möller Knut
Published: May 2022

Introduction

Coronavirus disease-2019 (COVID-19) pneumonia has different phenotypes. Selecting the patient individualized and optimal respirator settings for the ventilated patient is a challenging process. Electric impedance tomography (EIT) is a real-time, radiation-free functional imaging technique that can aid clinicians in differentiating the “low” (L-) and “high” (H-) phenotypes of COVID-19 pneumonia described previously.

Methods

Two patients (“A” and “B”) underwent a stepwise positive end-expiratory pressure (PEEP) recruitment by 3 cmH2O of steps from PEEP 10 to 25 and back to 10 cmH2O during a pressure control ventilation of 15 cmH2O. Recruitment maneuvers were performed under continuous EIT recording on a daily basis until patients required controlled ventilation mode.

Results

Patients “A” and “B” had a 7- and 12-day long trial, respectively. At the daily baseline, patient “A” had significantly higher compliance: mean ± SD = 53 ± 7 vs. 38 ± 5 ml/cmH2O (p < 0.001) and a significantly higher physiological dead space according to the Bohr–Enghoff equation than patient “B”: mean ± SD = 52 ± 4 vs. 45 ± 6% (p = 0.018). Following recruitment maneuvers, patient “A” had a significantly higher cumulative collapse ratio detected by EIT than patient “B”: mean ± SD = 0.40 ± 0.08 vs. 0.29 ± 0.08 (p = 0.007). In patient “A,” there was a significant linear regression between the cumulative collapse ratios at the end of the recruitment maneuvers (R2 = 0.824, p = 0.005) by moving forward in days, while not for patient “B” (R2 = 0.329, p = 0.5).

Conclusion

Patient “B” was recognized as H-phenotype with high elastance, low compliance, higher recruitability, and low ventilation-to-perfusion ratio; meanwhile patient “A” was identified as the L-phenotype with low elastance, high compliance, and lower recruitability. Observation by EIT was not just able to differentiate the two phenotypes, but it also could follow the transition from L- to H-type within patient “A.”

Clinical Trial Registration

www.ClinicalTrials.gov, identifier: NCT04360837.
Increased Cardiovascular Risk in Psoriatic Arthritis: Results From a Case-Control Monocentric Study
Authors: Degboé Yannick , Koch Richard , Zabraniecki Laurent , Jamard Bénédicte , Couture Guillaume , Ruidavets Jean Bernard , Ferrieres Jean , Ruyssen-Witrand Adeline , Constantin Arnaud
Published: May 2022

Background

Psoriatic arthritis (PsA) is associated with increased cardiovascular morbidity and mortality. The aims of our real-life study were to compare the prevalence of cardiovascular risk factors (CVRFs) and cardiovascular events (CVEs) among patients with PsA with a control population, to evaluate the impact of correcting factors in equations that assess cardiovascular risk (CVR) in PsA, and to determine the percentage of patients who reach the LDLc target as indicated by the European guidelines.

Methods

In this observational cross-sectional monocentric case-control study, we used a standardized procedure to systematically assess patients with PsA aged 25–85 years who met the Classification for Psoriatic Arthritis (CASPAR) criteria. Controls were extracted from the MOnitoring NAtionaL du rISque Artériel (MONALISA) study. We compared the prevalence of CVRFs, CVEs, the CVR, and the percentage of patients reaching recommended LDLc target in both populations. The CVR was first assessed using SCORE and QRISK2 equations. Then, the SCORE equation was corrected by applying a 1.5 multiplication factor, as recommended by EULAR for rheumatoid arthritis (SCORE-PsA), and the QRISK2 was corrected using the “rheumatoid arthritis” item (QRISK2-PsA).

Results

A total of 207 PsA and 414 controls were included. CVRFs and CVEs were more frequent in the PsA group. After controlling for age and gender, atherothrombotic disease was increased in the PsA population (SCORE p = 0.002, QRISK2 p = 0.001). Using the SCORE-PsA increased the percentage of patients with a high or very high CVR from 39.3 to 45.3% in the PsA group. Similarly, using the QRISK2-PsA increased the percentage of patients with a CVR ≥ 10% from 44.9 to 53.2%. The percentages of patients with PsA with high LDLc in the high and very high CVR groups were not significantly different from controls, despite a trend in favor of patients with PsA. Of the 83 PsA with a QRISK2 ≥ 10%, only 22.9% were treated with statin vs. 35.8% of the 134 controls. The QRISK2-PsA score did not alter these results.

Conclusion

In real-life, patients with PsA have a higher prevalence of CVRFs, as well as a higher prevalence of CVEs compared to the general population. The CVR is higher in the PsA population than in the controls either using the SCORE and QRISK2 equations or using the corrected SCORE- PsA and QRISK2-PsA equations.
Associations of Gut Microbiota and Fatty Metabolism With Immune Thrombocytopenia
Authors: Yu Xiaomin , Zheng Qingyun , He Yun , Yu Dandan , Chang Guolin , Chen Cheng , Bi Laixi , Lv Jia , Zhao Misheng , Lin Xiangyang , Zhu Liqing
Published: May 2022

Objective

To determine whether gut microbiota, fatty metabolism and cytokines were associated with immune thrombocytopenia (ITP).

Methods

In total, 29 preliminarily diagnosed ITP patients and 33 healthy volunteers were enrolled. Fecal bacterial were analyzed based on 16S rRNA sequencing. Plasma cytokines and motabolites were analyzed using flow cytometry and liquid chromatography-mass spectrometry (LC-MS), respectively.

Results

Bacteroides, Phascolarctobacterium, and Lactobacillus were enriched at the genus level in ITP patients, while Ruminococcaceae UCG-002, Eubacterium coprostanoligeues, Megamonas, and Lachnospiraceae NC2004 were depleted. At the phylum level, the relative abundance of Proteobacteria and Chloroflexi increased in ITP patients, while Firmicutes, Actinobacteria, and the Firmicutes/Bacteroidetes ratio decreased. Plasma levels of 5-hydroxyeicosatetraenoic acid (5-HETE), 6-trans-12-epi-leukotriene B4 (6t,12e-LTB4), and resolvin D2 (RvD2) were upregulated, and stachydrine, dowicide A, dodecanoylcarnitine were downregulated in ITP patients. Furthermore, RvD2 is positively correlated with order Bacteroidetes VC2.1 Bac22, 5-HETE is positively correlated with genus Azospirillum, and 6t,12e-LTB4 is positively correlated with genus Cupriavidus. In addition, stachydrine is positively correlated with family Planococcaceae, dowicide A is positively correlated with class MVP-15, and dodecanoylcarnitine is positively correlated with order WCHB1-41. Plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were upregulated in ITP patients.

Conclusion

Our study revealed a relationship between microbiota and fatty metabolism in ITP. Gut microbiota may participate in the pathogenesis of ITP through affecting cytokine secretion, interfering with fatty metabolism.
Clinical, Laboratory, and Electrocardiographic Findings in Colchicine Toxicity: 10 Years of Experience
Authors: Sheibani Mehdi , Zamani Nasim , Gerami Amir Hushang , Akhondi Hossein , Hassanian-Moghaddam Hossein
Published: May 2022

Background:

We aimed to investigate the clinical, laboratory, and electrocardiographic (ECG) findings of colchicine poisoning and to evaluate if there is a correlation between them and the two major outcomes of this toxicity which are respiratory/cardiovascular failure and death.

Materials and Methods

Medical records of 34 colchicine-intoxicated patients that were treated in our center during the past 10 years were retrospectively evaluated. The patient's clinical presentation, vital signs, laboratory tests, ECGs, and outcomes were reviewed.

Results

Abdominal pain, and hypotension at presentation had significant correlation with mortality (p = 0.003, OR: 2.2 [4.1, 7.9], p = 0.029, OR: 13.0 [1.5, 111.8]). Mortality significantly occurred in those with sinus tachycardia, hypokalemia, metabolic acidosis, and impaired liver and kidney function tests (p-values = 0.025, 0.007, 0.04, and 0.008, respectively). All the patients had some ECG abnormalities. Most frequent ECG abnormalities were pathologic ST segment elevation and depression (70%), left atrial enlargement (48%), and sinus tachycardia (37%), PR elevation in aVR lead (37%), and T wave inversion (37%).

Conclusions

Colchicine toxicity is a dangerous entity regarding the cardiovascular events and requires close general and cardiac monitoring.
Relationship Between Tamsulosin Use and Surgical Complications of Cataract Surgery in Elderly Patients: Population-Based Cohort Study
Authors: Kwak Jiehoon , Han Jung Yeob , Moon Su Young , Nam Sanghyu , Kim Jae Yong , Tchah Hungwon , Lee Hun
Published: May 2022

Purpose

Although several previous studies have investigated the relationship between tamsulosin use and surgical complications of cataract surgery, no population-based cohort study has been conducted for the Asian population. We aimed to investigate the relationship between tamsulosin use and surgical complications of cataract surgery in the Korean elderly population.

Methods

This nationwide population-based retrospective cohort study included elderly patients (≥60 years) who had undergone cataract surgery in the period from 2003 to 2015. Baseline characteristics were age, sex, income, residence, and systemic, and ocular comorbidities (glaucoma, myopia, eye trauma, diabetes mellitus with ophthalmic manifestations, severe cataract, age-related macular degeneration). The exposure of interest was tamsulosin use within 1 year before cataract surgery. Logistic regression model was used to evaluate the relationship of tamsulosin use with surgical complications of cataract surgery.

Results

The rate of surgical complications of cataract surgery was 0.88% (375/42,539) in the non-tamsulosin group and 0.83% (71/8,510) in the tamsulosin group. The groups showed no significant difference in the risk of surgical complications of cataract surgery in the unadjusted model [odds ratio (OR) = 0.946; 95% confidence interval (CI):0.733–1.220; P = 0.669]. Additionally, tamsulosin use was not significantly associated with surgical complications of cataract surgery in the fully adjusted model accounting for age, income, residence, and systemic and ocular comorbidities (OR = 0.997; 95% CI: 0.749–1.325; P = 0.981).

Conclusions

The rate or risk of surgical complications of cataract surgery does not change with tamsulosin use. We suggest that better surgical techniques and surgeons' cognizance of the patient's tamsulosin use could improve surgical outcomes, without increasing surgical complications.
CO2 Laser-Assisted Sclerectomy vs. Microcatheter-Assisted Trabeculotomy in the Management of a Bilateral Congenital Ectropion Uveae With Glaucoma: A Case Report and Literature Review
Authors: Chen Min , Li Yuhang , Cheng Bo , Zhang Qi , Liu Xin , Wang Kaijun
Published: May 2022

Introduction

Bilateral congenital ectropion uveae (CEU) is rare syndrome, usually accompanied by refractory glaucoma. Proper and timely treatment was very important for the prognosis. The report aims to compare the long-term outcomes and complications between the two eyes after different approaches of surgery in a case of bilateral CEU with advanced glaucoma.

Case Presentation

The patient was a 20-year-old male with bilateral CEU and glaucoma. The intraocular pressure (IOP) was 48 mm Hg in the right eye (OD) and 52 mm Hg in the left eye (OS). The vertical cup-to-disc (C/D) ratio was nearly 1.0 in both eyes. Despite maximum medical therapy, the target IOP could not be achieved. Therefore, CO2 laser-assisted sclerectomy surgery (CLASS) was performed in OS, and the IOP was remarkably decreased. 1 month after the surgery, the IOP rebounded slightly and was controlled with a fixed-combination anti-glaucoma medicine. 3-month postoperatively, a YAG laser goniopuncture (LGP) was performed to enhance the IOP-lowing effect and the anti-glaucoma agent was discontinued. An ab externo microcatheter-assisted trabeculotomy (MAT) was performed in OD, and the IOP was also significantly decreased. During the follow-up period, the IOP was well controlled for both eyes without any medication. Shallow anterior chamber and complicated cataract developed in OS after CLASS, and there was no obvious late complication in OD after MAT.

Conclusions

To our knowledge, this was the first attempt to perform two different surgeries, CLASS and MAT, in both eyes of a single patient presented with bilateral CEU with glaucoma. Our results showed that the IOP was lower after CLASS, but there were potential complications such as shallow anterior chamber and complicated cataract. MAT could achieve a moderate IOP-lowing effect but had a higher safety. CLASS and MAT may be considered effective surgical options for the management of such patients.
SARS-CoV-2 Within-Host and in vitro Genomic Variability and Sub-Genomic RNA Levels Indicate Differences in Viral Expression Between Clinical Cohorts and in vitro Culture
Authors: Agius Jessica E. , Johnson-Mackinnon Jessica C. , Fong Winkie , Gall Mailie , Lam Connie , Basile Kerri , Kok Jen , Arnott Alicia , Sintchenko Vitali , Rockett Rebecca J.
Published: May 2022

Background

Low frequency intrahost single nucleotide variants (iSNVs) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) have been increasingly recognised as predictive indicators of positive selection. Particularly as growing numbers of SARS-CoV-2 variants of interest (VOI) and concern (VOC) emerge. However, the dynamics of subgenomic RNA (sgRNA) expression and its impact on genomic diversity and infection outcome remain poorly understood. This study aims to investigate and quantify iSNVs and sgRNA expression in single and longitudinally sampled cohorts over the course of mild and severe SARS-CoV-2 infection, benchmarked against an in vitro infection model.

Methods

Two clinical cohorts of SARS-CoV-2 positive cases in New South Wales, Australia collected between March 2020 and August 2021 were sequenced. Longitudinal samples from cases hospitalised due to SARS-CoV-2 infection (severe) (n = 16) were analysed and compared with cases that presented with SARS-CoV-2 symptoms but were not hospitalised (mild) (n = 23). SARS-CoV-2 genomic diversity profiles were also examined from daily sampling of culture experiments for three SARS-CoV-2 variants (Lineage A, B.1.351, and B.1.617.2) cultured in VeroE6 C1008 cells (n = 33).

Results

Intrahost single nucleotide variants were detected in 83% (19/23) of the mild cohort cases and 100% (16/16) of the severe cohort cases. SNP profiles remained relatively fixed over time, with an average of 1.66 SNPs gained or lost, and an average of 4.2 and 5.9 low frequency variants per patient were detected in severe and mild infection, respectively. sgRNA was detected in 100% (25/25) of the mild genomes and 92% (24/26) of the severe genomes. Total sgRNA expressed across all genes in the mild cohort was significantly higher than that of the severe cohort. Significantly higher expression levels were detected in the spike and the nucleocapsid genes. There was significantly less sgRNA detected in the culture dilutions than the clinical cohorts.

Discussion and Conclusion

The positions and frequencies of iSNVs in the severe and mild infection cohorts were dynamic overtime, highlighting the importance of continual monitoring, particularly during community outbreaks where multiple SARS-CoV-2 variants may co-circulate. sgRNA levels can vary across patients and the overall level of sgRNA reads compared to genomic RNA can be less than 1%. The relative contribution of sgRNA to the severity of illness warrants further investigation given the level of variation between genomes. Further monitoring of sgRNAs will improve the understanding of SARS-CoV-2 evolution and the effectiveness of therapeutic and public health containment measures during the pandemic.
The Origin, Epidemiology, and Phylodynamics of Human Immunodeficiency Virus Type 1 CRF47_BF
Authors: Hill Gracelyn , Pérez-Losada Marcos , Delgado Elena , Benito Sonia , Montero Vanessa , Gil Horacio , Sánchez Mónica , Cañada-García Javier E. , García-Bodas Elena , Crandall Keith A. , Thomson Michael M. ,   the Spanish Group for the Study of New HIV Diagnoses
Published: May 2022

CRF47_BF is a circulating recombinant form (CRF) of the human immunodeficiency virus type 1 (HIV-1), the etiological agent of AIDS. CRF47_BF represents one of 19 CRFx_BFs and has a geographic focus in Spain, where it was first identified in 2010. Since its discovery, CRF47_BF has expanded considerably in Spain, predominantly through heterosexual contact (∼56% of the infections). Little is known, however, about the origin and diversity of this CRF or its epidemiological correlates, as very few samples have been available so far. This study conducts a phylogenetic analysis with representatives of all CRFx_BF sequence types along with HIV-1 M Group subtypes to validate that the CRF47_BF sequences share a unique evolutionary history. The CRFx_BF sequences cluster into a single, not well supported, clade that includes their dominant parent subtypes (B and F). This clade also includes subtype D and excludes sub-subtype F2. However, the CRF47_BF sequences all share a most recent common ancestor. Further analysis of this clade couples CRF47_BF protease-reverse transcriptase sequences and epidemiological data from an additional 87 samples collected throughout Spain, as well as additional CRF47_BF database sequences from Brazil and Spain to investigate the origin and phylodynamics of CRF47_BF. The Spanish region with the highest proportion of CRF47_BF samples in the data set was the Basque Country (43.7%) with Navarre next highest at 19.5%. We include in our analysis epidemiological data on host sex, mode of transmission, time of collection, and geographic region. The phylodynamic analysis indicates that CRF47_BF originated in Brazil around 1999–2000 and spread to Spain from Brazil in 2002–2003. The virus spread rapidly throughout Spain with an increase in population size from 2011 to 2015 and leveling off more recently. Three strongly supported clusters associated with Spanish regions (Basque Country, Navarre, and Aragon), together comprising 60.8% of the Spanish samples, were identified, one of which was also associated with transmission among men who have sex with men. The expansion in Spain of CRF47_BF, together with that of other CRFs and subtype variants of South American origin, previously reported, reflects the increasing relationship between the South American and European HIV-1 epidemics.