bluuebunny/crossref_metadata_2025 huggingface dataset:
A comparative study of semantic segmentation of omnidirectional images from a motorcycle perspective
Authors: Sekkat Ahmed Rida , Dupuis Yohan , Honeine Paul , Vasseur Pascal
Published: March 2022

The semantic segmentation of omnidirectional urban driving images is a research topic that has increasingly attracted the attention of researchers, because the use of such images in driving scenes is highly relevant. However, the case of motorized two-wheelers has not been treated yet. Since the dynamics of these vehicles are very different from those of cars, we focus our study on images acquired using a motorcycle. This paper provides a thorough comparative study to show how different deep learning approaches handle omnidirectional images with different representations, including perspective, equirectangular, spherical, and fisheye, and presents the best solution to segment road scene omnidirectional images. We use in this study real perspective images, and synthetic perspective, fisheye and equirectangular images, simulated fisheye images, as well as a test set of real fisheye images. By analyzing both qualitative and quantitative results, the conclusions of this study are multiple, as it helps understand how the networks learn to deal with omnidirectional distortions. Our main findings are that models with planar convolutions give better results than the ones with spherical convolutions, and that models trained on omnidirectional representations transfer better to standard perspective images than vice versa.
Multicolor labeling of airway neurons and analysis of parasympathetic heterogeneity
Authors: Pincus Alexandra B. , Huang Samuel J. , Lebold Katie M. , De La Torre Ubaldo , Proskocil Becky J. , Drake Matthew G. , Nakai Hiroyuki , Fryer Allison D. , Jacoby David B.
Published: March 2022

We report subpopulations of airway parasympathetic neurons expressing substance P, neuronal nitric oxide synthase, and tyrosine hydroxylase, highlighting unexplored heterogeneity in this population. These neurotransmitter-specific subpopulations did not form intraganglionic interneurons, but rather, extended outside the ganglia, into the airways, to distant innervation targets. Our experiments demonstrate the utility of multicolor labeling to characterize airway innervation, allowing us to confirm the extensive heterogeneity of postganglionic parasympathetic neurons. These methods will facilitate future investigations of neurophysiology and neural contributions to airway disease.
The influence of temperature environmental on performance of HNIW/FOX-7 based PBXs
Authors: Zhou Mengnan , Chen Shusen , Chao Hui , Wang Na , Yan Bo , Lan Guanchao , Jin Shaohua
Published: March 2022

During application, energetic materials may suffer different temperature environmental stimulation. In order to study the influence of temperature environmental on performance of HNIW/FOX-7 based PBXs, HNIW/FOX-7 based PBX modeling powders and PBX columns were treated by LT (low temperature), HT (high temperature), HLC (high-low temperature cycle) and HLS (high-low temperature shock). Then scanning electron microscope (SEM), infrared spectra (IR), X-ray diffraction (XRD) and differential scanning calorimetry (DSC) were used to study the variation of PBX modeling powders after LT, HT, HLC and HLS treatments; in addition, the mass, size and mechanical properties of PBX columns were characterized after different temperature adaptability treatments as well. The results indicate that the change ratios of mass and size of HNIW/FOX-7 based PBX columns are less than 1%, illustrating that mass and size of PBX columns are at acceptable level after different temperature adaptability treatments. The unevenness degree of the surface of PBX modeling powders followed the order of HLC > HT > LT > HLS, which agrees well with mass loss order. Moreover, IR and XRD results indicated that the molecular structure and crystal form of HNIW and FOX-7 did not change after different temperature adaptability treatments. Additionally, thermal stabilities of PBX modeling powders are decreased after different temperature adaptability treatments, among which HLS has the largest influence on HNIW/FOX-based PBX modeling powders. The compression strengths and elastic moduli of HNIW/FOX-based PBX columns are enhanced after different temperature adaptability treatments, among which the strength of PBX columns after HLC has the maximum increase, indicating that HLC has more significant effect on mechanical property.
Collective responses of bacteria to a local source of conflicting effectors
Authors: Livne Nir , Vaknin Ady
Published: March 2022

To cope in complex environments, motile bacteria have developed a chemosensory system that integrates multiple cues and directs their motion toward regions that it deems favorable. However, we have a limited understanding of the principles that govern bacterial behavior in complex stimuli fields. Here, we followed the spatial redistribution of E. coli cells in perplexing environments created by a local source of both beneficial (nutrients) and hazardous (low pH or indole) effectors. We identified two fundamentally distinct collective responses: a ‘trade-off’ response, in which bacteria sharply accumulated at a distance from the source that reflected a trade-off between the propagating effectors, and a ‘bet-hedging’ response, in which part of the bacteria accumulated away from the source, avoiding the hazardous effector, while the other part evaded the repulsive force and accumulated at the source. In addition, we demonstrate that cells lacking the Tsr sensor swim toward both repellents and, surprisingly, even toward pH values well below 7. Using a numerical analysis, we could correlate the collective bacterial responses with fundamentally distinct chemotactic force fields created along the channel by the propagation of the effectors and their unique perception by the chemosensory system.
Effects of plastic mulching on soil CO2 efflux in a cotton field in northwestern China
Authors: Zhao Zhimin , Shi Fengxia , Guan Fachun
Published: March 2022

In Northwestern China, more and more traditional cultivation system (TC) with no mulching and flood irrigation have been replaced by modern cultivation technology (MC) combining plastic film mulching with drip irrigation. Does plastic film mulching increase or reduce soil CO2 emission in arid areas? In order to study the effects of plastic mulching on soil CO2 efflux, a field study was conducted to compare soil CO2 concentration, soil CO2 efflux, soil temperature and moisture between the TC treatment and the MC treatment during a cotton growing season in Northwestern China. The seasonal patterns of soil profile temperature and soil moisture in the TC treatment were similar to that in the MC treatment. The mean value of soil profile temperature in the MC treatment was higher than that in the TC treatment. Except for soil moisture at 15 cm depth, the mean value of soil moisture at 5 cm and 10 cm depths in the MC treatment was higher than that in the TC treatment. The variation patterns of soil CO2 concentration and soil CO2 efflux in MC treatment were different to that in the TC treatment. Although the peak of soil CO2 concentration in the TC treatment was earlier than that in the MC treatment, the duration of soil CO2 concentration with high values in TC treatment was shorter than that in the MC treatment. Based on the model of Fick’s first diffusion law, soil surface CO2 efflux in the MC and TC treatments were determined. The surface CO2 efflux in the TC treatment calculated by Fick’s first diffusion law model was in good agreement with the value measured by chamber method. The seasonal curve of soil surface CO2 efflux in the MC treatment indicate the similar pattern with that in the TC treatment, and the rate of CO2 efflux was lower in the MC system. In the MC treatment, the seasonal variation of soil surface efflux was explained more by soil moisture than by soil temperature. However, in the TC treatment, the seasonal variation of soil surface efflux was explained more by soil temperature than by soil moisture. Over the completely experimental period, accumulated rates of soil CO2 efflux were 361 g C m−2 and 474 g C m−2 for the MC and TC system, respectively. We concluded that converting agricultural practices from traditional cultivation to the plastic mulching cultivation could reduce soil CO2 efflux by approximately 110 g C m−2 year−1 in agricultural land in arid areas of Northwestern China.
The gut microbiome of exudivorous marmosets in the wild and captivity
Authors: Malukiewicz Joanna , Cartwright Reed A. , Dergam Jorge A. , Igayara Claudia S. , Kessler Sharon E. , Moreira Silvia B. , Nash Leanne T. , Nicola Patricia A. , Pereira Luiz C. M. , Pissinatti Alcides , Ruiz-Miranda Carlos R. , Ozga Andrew T. , Quirino Adriana A. , Roos Christian , Silva Daniel L. , Stone Anne C. , Grativol Adriana D.
Published: March 2022

Mammalian captive dietary specialists like folivores are prone to gastrointestinal distress and primate dietary specialists suffer the greatest gut microbiome diversity losses in captivity compared to the wild. Marmosets represent another group of dietary specialists, exudivores that eat plant exudates, but whose microbiome remains relatively less studied. The common occurrence of gastrointestinal distress in captive marmosets prompted us to study the Callithrix gut microbiome composition and predictive function through bacterial 16S ribosomal RNA V4 region sequencing. We sampled 59 wild and captive Callithrix across four species and their hybrids. Host environment had a stronger effect on the gut microbiome than host taxon. Wild Callithrix gut microbiomes were enriched for Bifidobacterium, which process host-indigestible carbohydrates. Captive marmoset guts were enriched for Enterobacteriaceae, a family containing pathogenic bacteria. While gut microbiome function was similar across marmosets, Enterobacteriaceae seem to carry out most functional activities in captive host guts. More diverse bacterial taxa seem to perform gut functions in wild marmosets, with Bifidobacterium being important for carbohydrate metabolism. Captive marmosets showed gut microbiome composition aspects seen in human gastrointestinal diseases. Thus, captivity may perturb the exudivore gut microbiome, which raises implications for captive exudivore welfare and calls for husbandry modifications.
Glyoxylate protects against cyanide toxicity through metabolic modulation
Authors: Nielson Jason R. , Nath Anjali K. , Doane Kim P. , Shi Xu , Lee Jangwoen , Tippetts Emily G. , Saha Kusumika , Morningstar Jordan , Hicks Kevin G. , Chan Adriano , Zhao Yanbin , Kelly Amy , Hendry-Hofer Tara B. , Witeof Alyssa , Sips Patrick Y. , Mahon Sari , Bebarta Vikhyat S. , Davisson Vincent Jo , Boss Gerry R. , Rutter Jared , MacRae Calum A. , Brenner Matthew , Gerszten Robert E. , Peterson Randall T.
Published: March 2022

Although cyanide’s biological effects are pleiotropic, its most obvious effects are as a metabolic poison. Cyanide potently inhibits cytochrome c oxidase and potentially other metabolic enzymes, thereby unleashing a cascade of metabolic perturbations that are believed to cause lethality. From systematic screens of human metabolites using a zebrafish model of cyanide toxicity, we have identified the TCA-derived small molecule glyoxylate as a potential cyanide countermeasure. Following cyanide exposure, treatment with glyoxylate in both mammalian and non-mammalian animal models confers resistance to cyanide toxicity with greater efficacy and faster kinetics than known cyanide scavengers. Glyoxylate-mediated cyanide resistance is accompanied by rapid pyruvate consumption without an accompanying increase in lactate concentration. Lactate dehydrogenase is required for this effect which distinguishes the mechanism of glyoxylate rescue as distinct from countermeasures based solely on chemical cyanide scavenging. Our metabolic data together support the hypothesis that glyoxylate confers survival at least in part by reversing the cyanide-induced redox imbalances in the cytosol and mitochondria. The data presented herein represent the identification of a potential cyanide countermeasure operating through a novel mechanism of metabolic modulation.
Applying the perturbative integral in aeromaneuvers around Mars to calculate the cost
Authors: Piñeros Jhonathan O. Murcia , de Almeida Prado Antônio F. Bertachini , dos Santos Walter Abrahão , de Moraes Rodolpho Vilhena
Published: March 2022

The perturbative integral method was applied to quantify the contribution of external forces during a specific interval of time in trajectories of spacecraft around asteroids and under the Luni-solar influence. However, this method has not been used to quantify the contributions of drag in aerocapture and aerobraking. For this reason, the planet Mars is selected to apply this method during an aerogravity-assisted maneuver. Several trajectories are analyzed, making use of a drag device with area to mass ratios varying from 0.0 to 20.0 m2/kg, simulating solar sails or de-orbit devices. The mathematical model is based in the restricted three-body problem. The use of this maneuver makes it possible to obtain the variations of energy in the trajectory, replacing expensive maneuvers based on fuel consumption. To observe the effects of the maneuvers, different values of pericenter velocity and altitude were selected for prograde and retrograde orbits. The innovation of this research is the application of an integral method to quantify the delta-V of the aero gravity maneuver, comparing the cost of the maneuver with the traditional methods of space propulsion. The results allow the identification of orbits with conditions to capture, and the perturbative maps show the velocity variations.
A nationwide study of the risks of major mental disorders among the offspring of parents with rheumatoid arthritis
Authors: Chiu Hsien-Jane , Sun Cheuk-Kwan , Tsai Shih-Jen , Bai Ya-Mei , Hung Kuo-Chuan , Hsu Ju-Wei , Huang Kai-Lin , Su Tung-Ping , Chen Tzeng-Ji , Sun Andrew , Cheng Yu-Shian , Chen Mu-Hong
Published: March 2022

Rheumatoid arthritis (RA) may share genomic risks with certain mental disorders. This study aimed at investigating associations between parental RA and risks of mental disorders in offspring. Using the National Health Insurance Research Database (2001–2010), we conducted a matched cohort study involving two parent–child cohorts (i.e., RA-parent–child cohort and non-RA-parent–child cohort) between which risks of major mental disorders in offspring were compared. There were 23,981 parent–child pairs in the RA-parent–child cohort and 239,810 in the non-RA-parent–child cohort. Preliminary analysis demonstrated increased risks of autism spectrum disorders (ASDs) [Odds ratio (OR) 1.47; 95% confidence interval (CI) 1.05–2.07], attention-deficit/hyperactivity disorder (ADHD) [OR 1.34; (95% CI 1.17–1.54)], bipolar disorder [OR 1.41 (95% CI 1.17–1.70)], and major depressive disorder [OR 1.20 (95% CI 1.07–1.35)] associated with parental RA. Sub-group analysis further showed higher risks of the four disorders in children of mothers with RA but not those from fathers with RA. Higher risks of ASDs and ADHD were not noted in children of mothers with RA before childbirth. Maternal RA, but not paternal RA or mothers diagnosed with RA before childbirth, was associated with increased risks of multiple mental disorders in their offspring, suggesting potential contributions of maternal genetic factors to ASDs and ADHD development in offspring.
Upregulation of Cytotoxic T-cells in pediatric patients with Gaucher disease
Authors: Zahran Asmaa M. , Saad Khaled , Elsayh Khalid I. , Abdou Madleen Adel A. , Abo-Elgheet Amir M. , Eloseily Esraa M. , Khalaf Shaimaa M. , Sror Shabaan , Ahmad Faisal-Alkhateeb , Elhoufey Amira , Ghandour Aliaa , Osman Naglaa S.
Published: March 2022

Cytotoxic (CD8) T-cells and natural killer (NK) cells have a significant immune function role. The ongoing stimulation of immunity and the excessive release of proinflammatory cytokines observed in pediatric patients with Gaucher disease (GD) can affect immune cells. Few studies have looked at the proportion of cytotoxic CD8 T-cells and their subsets in children with GD. A prospective case–control study was performed involving twenty pediatric patients with type 1 GD and twenty healthy age-matched controls. All patients received regular enzyme replacement therapy (ERT) for at least 6 months before the study. Complete blood count and flow cytometric analyses of CD8 T, Tc1, Tc2, NK, and NK T-cells were performed. GD patients showed significantly increased of CD8 T, Tc1 and significantly decreased NK cells frequencies when compared to healthy controls. However, no significant difference in Tc2 and NK T-cells was found between the studied groups. GD patients on regular ERT have increased CD8+ T-cell frequencies, predominantly Tc1, together with a reduction in NK cells than in healthy controls. These crucial immunological changes may contribute to some extent to the pathogenesis and the progression of GD.
Altered proteome in translation initiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage
Authors: Ram Anup Kumar , Mallik Monalisha , Reddy R. Rajendra , Suryawanshi Amol Ratnakar , Alone Pankaj V.
Published: March 2022

The recognition of the AUG start codon and selection of an open reading frame (ORF) is fundamental to protein biosynthesis. Defect in the fidelity of start codon selection adversely affect proteome and have a pleiotropic effect on cellular function. Using proteomic techniques, we identified differential protein abundance in the translation initiation fidelity defective eIF5G31R mutant that initiates translation using UUG codon in addition to the AUG start codon. Consistently, the eIF5G31R mutant altered proteome involved in protein catabolism, nucleotide biosynthesis, lipid biosynthesis, carbohydrate metabolism, oxidation–reduction pathway, autophagy and re-programs the cellular pathways. The utilization of the upstream UUG codons by the eIF5G31R mutation caused downregulation of uridylate kinase expression, sensitivity to hydroxyurea, and DNA damage. The eIF5G31R mutant cells showed lower glutathione levels, high ROS activity, and sensitivity to H2O2.
Quinoline based thiosemicarbazones as colorimetric chemosensors for fluoride and cyanide ions and DFT studies
Authors: Basri Rabia , Ahmed Nadeem , Khalid Muhammad , Khan Muhammad Usman , Abdullah Muhammad , Syed Asad , Elgorban Abdallah M. , Al-Rejaie Salim S. , Braga Ataualpa Albert Carmo , Shafiq Zahid
Published: March 2022

High toxicity and extensive accessibility of fluoride and cyanide ions in diverse environmental media encouraged attention for scheming well-organized probes for their detection. Keeping in mind we have designed and synthesized thiosemicarbazone-based chemosensors RB-1, RB-2 and RB-3 for the detection of fluoride and cyanide ions. The structural elucidation of the synthesized chemosensors is done by employing different analytical techniques including nuclear magnetic resonance and electronic absorption specrtoscopies. Admirable detection limit, binding constant and fast response time (2 s) to F− and CN− ions enlarged the applications of these chemosensors. Additional confirmation of the sensing ability of these chemosensors is derived from DFT and TDDFT calculations with M06/6-311G(d,p) method by performing FMO, UV–Vis, QTAIM and global reactivity parameters elucidation. Overall results point out that investigated chemosensors are suitable candidates for sensing the F− ions. These chemosensors were successfully applied to detect F− ions in a commercial toothpaste sample.
An improved adaptive neuro fuzzy inference system model using conjoined metaheuristic algorithms for electrical conductivity prediction
Authors: Ahmadianfar Iman , Shirvani-Hosseini Seyedehelham , He Jianxun , Samadi-Koucheksaraee Arvin , Yaseen Zaher Mundher
Published: March 2022

Precise prediction of water quality parameters plays a significant role in making an early alert of water pollution and making better decisions for the management of water resources. As one of the influential indicative parameters, electrical conductivity (EC) has a crucial role in calculating the proportion of mineralization. In this study, the integration of an adaptive hybrid of differential evolution and particle swarm optimization (A-DEPSO) with adaptive neuro fuzzy inference system (ANFIS) model is adopted for EC prediction. The A-DEPSO method uses unique mutation and crossover processes to correspondingly boost global and local search mechanisms. It also uses a refreshing operator to prevent the solution from being caught inside the local optimal solutions. This study uses A-DEPSO optimizer for ANFIS training phase to eliminate defects and predict accurately the EC water quality parameter every month at the Maroon River in the southwest of Iran. Accordingly, the recorded dataset originated from the Tange-Takab station from 1980 to 2016 was operated to develop the ANFIS-A-DEPSO model. Besides, the wavelet analysis was jointed to the proposed algorithm in which the original time series of EC was disintegrated into the sub-time series through two mother wavelets to boost the prediction certainty. In the following, the comparison between statistical metrics of the standalone ANFIS, least-square support vector machine (LSSVM), multivariate adaptive regression spline (MARS), generalized regression neural network (GRNN), wavelet-LSSVM (WLSSVM), wavelet-MARS (W-MARS), wavelet-ANFIS (W-ANFIS) and wavelet-GRNN (W-GRNN) models was implemented. As a result, it was apparent that not only was the W-ANFIS-A-DEPSO model able to rise remarkably the EC prediction certainty, but W-ANFIS-A-DEPSO (R = 0.988, RMSE = 53.841, and PI = 0.485) also had the edge over other models with Dmey mother in terms of EC prediction. Moreover, the W-ANFIS-A-DEPSO can improve the RMSE compared to the standalone ANFIS-DEPSO model, accounting for 80%. Hence, this model can create a closer approximation of EC value through W-ANFIS-A-DEPSO model, which is likely to act as a promising procedure to simulate the prediction of EC data.
Mining human preference via self-correction causal structure learning
Authors: Sun Jian , Wu Chenye , Peng Weihua , Huang Jiayan , Han Cuiyun , Zhu Yong , Lyu Yajuan
Published: March 2022

Spurred by causal structure learning (CSL) ability to reveal the cause–effect connection, significant research efforts have been made to enhance the scalability of CSL algorithms in various artificial intelligence applications. However, less effort has been made regarding the stability and the interpretability of CSL algorithms. Thus, this work proposes a self-correction mechanism that embeds domain knowledge for CSL, improving the stability and accuracy even in low-dimensional but high-noise environments by guaranteeing a meaningful output. The suggested algorithm is challenged against multiple classic and influential CSL algorithms in synthesized and field datasets. Our algorithm achieves a superior accuracy on the synthesized dataset, while on the field dataset, our method interprets the learned causal structure as a human preference for investment, coinciding with domain expert analysis.
Synthesis and characterization of highly efficient and recoverable Cu@MCM-41-(2-hydroxy-3-propoxypropyl) metformin mesoporous catalyst and its uses in Ullmann type reactions
Authors: Robatjazi Zahra S. , Naimi-Jamal M. Reza , Tajbakhsh Mahdieh
Published: March 2022

The functionalized MCM-41-(2-hydroxy-3-propoxypropyl) metformin was prepared and anchored by copper ions to employ as a catalyst for the Ullmann C-X coupling reaction. The catalyst was characterized by Fourier-transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy measurements and, N2 adsorption–desorption isotherms. The benefits of this catalyst are the use of inexpensive and non-toxic metformin ligand, easy catalyst/product separation, and catalyst recycling. The catalyst can be reused at least for five repeated cycles without a significant loss of its catalytic activity or metal leaching.
Identification of microRNAs associated with human fragile X syndrome using next-generation sequencing
Authors: Sotoudeh Anvari Maryam , Vasei Hamed , Najmabadi Hossein , Badv Reza Shervin , Golipour Akram , Mohammadi-Yeganeh Samira , Salehi Saeede , Mohamadi Mahmood , Goodarzynejad Hamidreza , Mowla Seyed Javad
Published: March 2022

Fragile X syndrome (FXS) is caused by a mutation in the FMR1 gene which can lead to a loss or shortage of the FMR1 protein. This protein interacts with specific miRNAs and can cause a range of neurological disorders. Therefore, miRNAs could act as a novel class of biomarkers for common CNS diseases. This study aimed to test this theory by exploring the expression profiles of various miRNAs in Iranian using deep sequencing-based technologies and validating the miRNAs affecting the expression of the FMR1 gene. Blood samples were taken from 15 patients with FXS (9 males, 6 females) and 12 controls. 25 miRNAs were differentially expressed in individuals with FXS compared to controls. Levels of 9 miRNAs were found to be significantly changed (3 upregulated and 6 downregulated). In Patients, the levels of hsa-miR-532-5p, hsa-miR-652-3p and hsa-miR-4797-3p were significantly upregulated while levels of hsa-miR-191-5p, hsa-miR-181-5p, hsa-miR-26a-5p, hsa-miR-30e-5p, hsa-miR-186-5p, and hsa-miR-4797-5p exhibited significant downregulation; and these dysregulations were confirmed by RT‐qPCR. This study presents among the first evidence of altered miRNA expression in blood samples from patients with FXS, which could be used for diagnostic, prognostic, and treatment purposes. Larger studies are required to confirm these preliminary results.
Energy transfer of trapped electron turbulence in tokamak fusion plasmas
Authors: Qi Lei
Published: March 2022

The first principle gyrokinetic simulations of trapped electron turbulence in tokamak fusion plasmas demonstrate the energy transfers from the most linearly unstable modes at high $$k_\theta \rho _i\sim 1$$

k
θ

ρ
i

∼
1

to intermediate $$k_\theta$$

k
θ

via parametric decay process in a short period of linear-nonlinear transition phase. Dominant nonlinear wave-wave interactions occur near the mode rational surface $$m\simeq nq$$

m
≃
n
q

. In fully nonlinear turbulence, inverse energy cascade occurs between a cutoff wave number $$k_c$$

k
c

and $$k_\theta \rho _i\sim 1$$

k
θ

ρ
i

∼
1

with a power law scaling $$|\phi (k_\theta )|^2\propto k^{-3}$$

|
ϕ

(

k
θ

)

|

2

∝

k

-
3

, while modes with $$k<k_c$$

k
<

k
c

are suppressed. The numerical findings show fair agreement with both the weak turbulence theory and realistic experiments on Tore Supra tokamak.
A lensed fiber Bragg grating-based membrane-in-the-middle optomechanical cavity
Authors: Baraillon Joris , Taurel Boris , Labeye Pierre , Duraffourg Laurent
Published: March 2022

Optomechanical systems benefit from the coupling between an optical field and mechanical vibrations. Fiber-based devices are well suited to easily exploit this interaction. We report an alternative approach of a silicon nitride membrane-in-the-middle of a high quality factor ($$10^6$$

10
6

–$$10^7$$

10
7

) Fabry–Perot, formed by a grating inscribed within a fiber core as an input mirror in front of a dielectric back mirror. The Pound–Drever–Hall technique used to stabilize the laser frequency on the optical resonance frequency allows us to reduce the low frequency noise down to $${4}\,{{\mathrm{kHz}}/\sqrt{\mathrm{Hz}}}$$

4

kHz
/

Hz

. We present a detailed methodology for the characterization of the optical and optomechanical properties of this stabilized system, using various membrane geometries, with corresponding resonance frequencies in the range of several hundred of $${\mathrm{kHz}}$$
kHz
. The excellent long-term stability is illustrated by continuous measurements of the thermomechanical noise spectrum over several days, with the laser source maintained at optical resonance. This major result makes this system an ideal candidate for optomechanical sensing.
Comparison of soft tissue simulations between two planning software programs for orthognathic surgery
Authors: Modabber Ali , Baron Tanja , Peters Florian , Kniha Kristian , Danesh Golamreza , Hölzle Frank , Ayoub Nassim , Möhlhenrich Stephan Christian
Published: March 2022

The aim of this study was to compare the soft tissue predicative abilities of two established programs depending on the surgical technique and amount of displacement. On the basis of 50 computed tomography images, 11 orthognathic operations with differences in displacement distances and technique (maxillary advancement, MxA; maxillary impaction, MxI; mandibular setback, MnS; mandibular advancement, MnA bimaxillary displacement, MxA/MnS) as well as corresponding soft tissue predictions were simulated using the programs Dolphin (D) and ProPlan (PP). For all the soft tissue predictions by the two programs, eight linear and two angular measurements were performed and compared. The simulation of maxillary impaction showed a similar soft tissue behaviour between the two programs. However, differences or divergent behaviours were observed for other procedures. In the middle third of the face these significant differences concerned in particular the nasolabial angle (Ns-Sn-Ls)(5 mm-MA, D: 119.9 ± 8.6° vs. PP: 129.5 ± 8.4°; 7 mm-MnS: D: 128.5 ± 8.2° vs. PP: 129.6 ± 8.1°; 10 mm-MnA D: 126.0 ± 8.0° vs. PP: 124.9 ± 8.4°; 5 mm-MxA/4 mm-MnS, D: 120.2 ± 8.7° vs. PP: 129.9 ± 8.3°; all p < 0.001) and in the lower third the mentolabial angle (Pog´-B´-Li) (5 mm-MA, D: 133.2 ± 11.4° vs. PP: 126.8 ± 11.6°; 7 mm-MnS: D: 133.1 ± 11.3° vs. PP: 124.6 ± 11.9°; 10 mm-MnA D: 133.3 ± 11.5° vs. PP: 146.3 ± 11.1°; bignathic 5 mm-MxA/4 mm-MnS, D: 133.1 ± 11.4° vs. PP: 122.7 ± 11.9°; all p < 0.001) and the distance of the inferior lip to the aesthetic Line (E-Line-Li) (5 mm-MA, D: 3.7 ± 2.3 mm vs. PP: 2.8 ± 2.5 mm; 7 mm-MnS: D: 5.1 ± 3.0 mm vs. PP: 3.3 ± 2.3 mm; 10 mm-MnA D: 2.5 ± 1.6 mm vs. PP: 3.9 ± 2.8 mm; bignathic 5 mm-MxA/4 mm-MnS, D: 4.8 ± 3.0 mm vs. PP: 2.9 ± 2.0 mm; all p < 0.001). The soft tissue predictions by the tested programs differed in simulation outcome, which led to the different, even divergent, results. However, the significant differences are often below a clinically relevant level. Consequently, soft tissue prediction must be viewed critically, and its actual benefit must be clarified.
DNA methylation in Friedreich ataxia silences expression of frataxin isoform E
Authors: Rodden Layne N. , Gilliam Kaitlyn M. , Lam Christina , Rojsajjakul Teerapat , Mesaros Clementina , Dionisi Chiara , Pook Mark , Pandolfo Massimo , Lynch David R. , Blair Ian A. , Bidichandani Sanjay I.
Published: March 2022

Epigenetic silencing in Friedreich ataxia (FRDA), induced by an expanded GAA triplet-repeat in intron 1 of theFXNgene, results in deficiency of the mitochondrial protein, frataxin. A lesser known extramitochondrial isoform of frataxin detected in erythrocytes, frataxin-E, is encoded via an alternate transcript (FXN-E) originating in intron 1 that lacks a mitochondrial targeting sequence. We show thatFXN-Eis deficient in FRDA, including in patient-derived cell lines, iPS-derived proprioceptive neurons, and tissues from a humanized mouse model. In a series of FRDA patients, deficiency of frataxin-E protein correlated with the length of the expanded GAA triplet-repeat, and with repeat-induced DNA hypermethylation that occurs in close proximity to the intronic origin ofFXN-E. CRISPR-induced epimodification to mimic DNA hypermethylation seen in FRDA reproducedFXN-Etranscriptional deficiency. Deficiency of frataxin E is a consequence of FRDA-specific epigenetic silencing, and therapeutic strategies may need to address this deficiency.
Despite its sequence identity with canonical H4, Drosophila H4r product is enriched at specific chromatin regions
Authors: Ábrahám Andrea , Villányi Zoltán , Zsindely Nóra , Nagy Gábor , Szabó Áron , Bodai László , Henn László , Boros Imre M.
Published: March 2022

Histone variants are different from their canonical counterparts in structure and are encoded by solitary genes with unique regulation to fulfill tissue or differentiation specific functions. A single H4 variant gene (His4r or H4r) that is located outside of the histone cluster and gives rise to a polyA tailed messenger RNA via replication-independent expression is preserved in Drosophila strains despite that its protein product is identical with canonical H4. In order to reveal information on the possible role of this alternative H4 we epitope tagged endogenous H4r and studied its spatial and temporal expression, and revealed its genome-wide localization to chromatin at the nucleosomal level. RNA and immunohistochemistry analysis of H4r expressed under its cognate regulation indicate expression of the gene throughout zygotic and larval development and presence of the protein product is evident already in the pronuclei of fertilized eggs. In the developing nervous system a slight disequibrium in H4r distribution is observable, cholinergic neurons are the most abundant among H4r-expressing cells. ChIP-seq experiments revealed H4r association with regulatory regions of genes involved in cellular stress response. The data presented here indicate that H4r has a variant histone function.
In vitro plant tissue culture as the fifth generation of bioenergy
Authors: Norouzi Omid , Hesami Mohsen , Pepe Marco , Dutta Animesh , Jones Andrew Maxwell P.
Published: March 2022

Developing and applying a novel and sustainable energy crop is essential to reach an efficient and economically feasible technology for bioenergy production. In this study, plant tissue culture, also referred to as in vitro culture, is introduced as one of the most promising and environmentally friendly methods for the sustainable supply of biofuels. The current study investigates the potential of in vitro -grown industrial hemp calli obtained from leaf, root, and stem explants as a new generation of energy crop. For this purpose, the in vitro grown explants were first fully characterized in terms of elemental and chemical composition. Secondly, HTL experiments were designed by Design Expert 11 with a particular focus on biocrude. Finally, the chemical components, functional groups, and petroleum-like hydrocarbons present in the biocrude were identified by PY-GCMS. A 22.61 wt.% biocrude was produced for the sample grown through callogenesis of the leaf (CL). The obtained biocrude for CL consisted of 19.55% acids, 0.42% N compounds, 15.44% ketones, 16.03% aldehydes, 2.21% furans, 20.01% aromatics, 5.2% alcohols, and 19.88% hydrocarbons. To the best of the authors’ knowledge, this is the first report that in vitro -grown biomass is hydrothermally liquefied toward biocrude production; the current work paves the way for integrating plant tissue culture and thermochemical processes for the generation of biofuels and value-added chemicals.
On the durability of surgical masks after simulated handling and wear
Authors: Varanges Vincent , Caglar Baris , Lebaupin Yann , Batt Till , He Weidong , Wang Jing , Rossi René M. , Richner Gilles , Delaloye Jean-Romain , Michaud Véronique
Published: March 2022

After the spread of COVID-19, surgical masks became highly recommended to the public. They tend to be handled and used multiple times, which may impact their performance. To evaluate this risk, surgical masks of Type IIR were submitted to four simulated treatments: folding, ageing with artificial saliva or sweat and washing cycles. The air permeability, mechanical integrity, electrostatic potential, and filtration efficiency (FE) of the masks were measured to quantify possible degradation. Overall, air permeability and mechanical integrity were not affected, except after washing, which slightly degraded the filtering layers. Electrostatic potential and FE showed a strong correlation, highlighting the role of electrostatic charges on small particle filtration. A slight decrease in FE for 100 nm particles was found, from 74.4% for the reference masks to 70.6% for the mask treated in saliva for 8 h. A strong effect was observed for washed masks, resulting in FE of 46.9% (± 9.5%), comparable to that of a control group with no electrostatic charges. A dry store and reuse strategy could thus be envisaged for the public if safety in terms of viral and bacterial charge is ensured, whereas washing strongly impacts FE and is not recommended.
Children transition from simple associations to explicitly reasoned social learning strategies between age four and eight
Authors: Blakey Kirsten H. , Renner Elizabeth , Atkinson Mark , Rafetseder Eva , Caldwell Christine A.
Published: March 2022

To differentiate the use of simple associations from use of explicitly reasoned selective social learning, we can look for age-related changes in children’s behaviour that might signify a switch from one social learning strategy to the other. We presented 4- to 8-year-old children visiting a zoo in Scotland (N = 109) with a task in which the perceptual access of two informants was determined by the differing opacity of two screens of similar visual appearance during a hiding event. Initially success could be achieved by forming an association or inferring a rule based on salient visual (but causally irrelevant) cues. However, following a switch in the scenario, success required explicit reasoning about informants’ potential to provide valuable information based on their perceptual access. Following the switch, older children were more likely to select a knowledgeable informant. This suggests that some younger children who succeeded in the pre-switch trials had inferred rules or formed associations based on superficial, yet salient, visual cues, whereas older children made the link between perceptual access and the potential to inform. This late development and apparent cognitive challenge are consistent with proposals that such capacities are linked to the distinctiveness of human cumulative culture.
LCZ696 ameliorates doxorubicin-induced cardiomyocyte toxicity in rats
Authors: Miyoshi Toru , Nakamura Kazufumi , Amioka Naofumi , Hatipoglu Omer F. , Yonezawa Tomoko , Saito Yukihiro , Yoshida Masashi , Akagi Satoshi , Ito Hiroshi
Published: March 2022

Doxorubicin (DOX)-based chemotherapy induces cardiotoxicity, which is considered the main bottleneck for its clinical application. In this study, we investigated the potential benefit of LCZ696, an angiotensin receptor–neprilysin inhibitor against DOX-induced cardiotoxicity in rats and H9c2 cells and determined whether the mechanism underlying any such effects involves its antioxidant activity. Male Sprague–Dawley rats were randomly separated into four groups, each consisting of 15 rats (DOX (1.5 mg/kg/day intraperitoneally for 10 days followed by non-treatment for 8 days); DOX + valsartan (31 mg/kg/day by gavage from day 1 to day 18); DOX + LCZ696 (68 mg/kg/day by gavage from day 1 to day 18); and control (saline intraperitoneally for 10 days). DOX-induced elevation of cardiac troponin T levels on day 18 was significantly reduced by LCZ696, but not valsartan. The DOX-induced increase in myocardial reactive oxygen species (ROS) levels determined using dihydroethidium was significantly ameliorated by LCZ696, but not valsartan, and was accompanied by the suppression of DOX-induced increase in p47phox. LCZ696 recovered the DOX-induced decrease in phosphorylation of adenosine monophosphate-activated protein kinase and increased the ratio of Bax and Bcl-2. In H9c2 cardiomyocytes, LCZ696 reduced DOX-induced mitochondrial ROS generation and improved cell viability more than valsartan. Our findings indicated that LCZ696 ameliorated DOX-induced cardiotoxicity in rat hearts in vivo and in vitro, possibly by mediating a decrease in oxidative stress.
Saffron extract and crocin exert anti-inflammatory and anti-oxidative effects in a repetitive mild traumatic brain injury mouse model
Authors: Salem Marwa , Shaheen Mariam , Tabbara Abeer , Borjac Jamilah
Published: March 2022

Saffron Crocus sativus L. (C. sativus) is a flower from the iridaceous family. Crocin, saffron’s major constituent, and saffron have anti-oxidative and anti-inflammatory activities. In this work, the neuroprotective effects of saffron and crocin are being investigated in a repetitive mild traumatic brain injury (rmTBI) mouse model. A weight drop model setup was employed to induce mild brain injury in male albino BABL/c mice weighing 30–40 g. Saffron (50 mg/kg) and crocin (30 mg/kg) were administrated intraperitoneally 30 min before mTBI induction. Behavioral tests were conducted to assess behavioral deficits including the modified neurological severity score (NSS), Morris water maze (MWM), pole climb test, rotarod test, and adhesive test. The levels of TNF alpha (TNF-α), interferon-gamma (IFN-γ), myeloperoxidase activity (MPO), malonaldehyde (MDA), and reduced glutathione (GSH) were measured. Histological analysis of different brain parts was performed. Both saffron and crocin demonstrated marked improved neurological, cognitive, motor, and sensorimotor functions. Besides, both compounds significantly reduced the oxidative stress and inflammatory processes. No abnormal histological features were observed in any of the injured groups. Saffron extract and crocin provide a neuroprotective effect in a mouse model of rmTBI by decreasing oxidative stress, inflammatory responses, and behavioral deficits.
Long-term nutritional trends in the Finnish population estimated from a large laboratory database from 1987 to 2020
Authors: Tuuminen Tamara , Sorsa Mikko , Tornudd Martin , Lähteenmäki Pertti Lauri , Poussa Tuija , Suonsivu Pyry , Pitkänen Eeva Marja , Antila Erkki , Jaakkola Kaarlo
Published: March 2022

The assessments of malnutrition in adults with MUST or NRS-2002 criteria do not give a detailed insight into the sufficiency of micronutrients. Sufficiency assessment of essential micronutrients on the individual level can be achieved only with laboratory measurements. The aim of this study was to estimate long-term trends in micronutrient sufficiency in the Finnish population with regards to gender and sex covariates. We retrieved from the clinical laboratory database (n = 67,236) all results on whole blood Magnesium, (B-Mg), Manganese (B-Mn), Zinc (B-Zn), Selenium (B-Se) and Copper from erythrocytes (E-Cu) and fasting serum β-carotenes (fS-BKarot), vitamin A (fS-A-vit), coenzyme Q10 (Ubiquinone, fS-Q10) and serum vitamin D (S-D-25) from the database of clinical laboratory Mineraalilaboratorio Mila Oy from the years 1987–2020. A weak positive linear trend is seen for B-Mg, B-Zn and ln(fS-Q10) both for children and adults, but a moderate linear positive trend was observed for ln(S-D-25) based on correlation between calendar year and ln(S-D-25), R = 0.44 and 0.41, p < 0.001 for adults and children, respectively. Laboratory database is helpful to monitor the nutritional public policy to prevent hidden malnutrition in the society.
Enhancing multiple scales of seafloor biodiversity with mussel restoration
Authors: Sea Mallory A. , Hillman Jenny R. , Thrush Simon F.
Published: March 2022

Restoration projects are underway internationally in response to global declines in shellfish beds. As diverse biological assemblages underpin a variety of ecosystem services, understanding broader changes in biodiversity associated with mussel restoration becomes increasingly valuable to scientists and restoration practitioners. Studies generally show bivalve beds increase species richness and abundance, but results are scale-dependent and conditional on the mobility of specific communities observed. We examined biodiversity at multiple scales to determine how communities with varying levels of mobility are influenced by subtidal mussel restoration. Significant changes in assemblage structure were observed in both mobile fish and epifaunal communities, with enhanced species richness and total abundance of associated individuals. In contrast, we observed site-dependent effects of bivalve restoration on macrofaunal community structure and composition, with sheltered, harbour mussel bed communities numerically dominated by detritivores accustomed to organically enriched, muddy sediments. Sediment organic matter significantly increased within mussel beds, and distance-based linear models showed that sediment organic matter was an important predictor of macrofaunal assemblage structure on mussel beds, highlighting the significance of benthic-pelagic coupling and biodeposition to soft-sediment communities. This study contributes novel methods and ecological insights on the role of species mobility and site selection in structuring restoration outcomes, better informing future mussel restoration efforts aimed at emphasising functionally-driven ecosystem services.
Megapixel camera arrays enable high-resolution animal tracking in multiwell plates
Authors: Barlow Ida L. , Feriani Luigi , Minga Eleni , McDermott-Rouse Adam , O’Brien Thomas James , Liu Ziwei , Hofbauer Maximilian , Stowers John R. , Andersen Erik C. , Ding Siyu Serena , Brown André E. X.
Published: March 2022

Tracking small laboratory animals such as flies, fish, and worms is used for phenotyping in neuroscience, genetics, disease modelling, and drug discovery. An imaging system with sufficient throughput and spatiotemporal resolution would be capable of imaging a large number of animals, estimating their pose, and quantifying detailed behavioural differences at a scale where hundreds of treatments could be tested simultaneously. Here we report an array of six 12-megapixel cameras that record all the wells of a 96-well plate with sufficient resolution to estimate the pose of C. elegans worms and to extract high-dimensional phenotypic fingerprints. We use the system to study behavioural variability across wild isolates, the sensitisation of worms to repeated blue light stimulation, the phenotypes of worm disease models, and worms’ behavioural responses to drug treatment. Because the system is compatible with standard multiwell plates, it makes computational ethological approaches accessible in existing high-throughput pipelines.
Spatio-temporal patterns of multi-trophic biodiversity and food-web characteristics uncovered across a river catchment using environmental DNA
Authors: Blackman Rosetta C. , Ho Hsi-Cheng , Walser Jean-Claude , Altermatt Florian
Published: March 2022

Accurate characterisation of ecological communities with respect to their biodiversity and food-web structure is essential for conservation. However, combined empirical study of biodiversity and multi-trophic food webs at a large spatial and temporal resolution has been prohibited by the lack of appropriate access to such data from natural systems. Here, we assessed biodiversity and food-web characteristics across a 700 km2riverine network over seasons using environmental DNA. We found contrasting biodiversity patterns between major taxonomic groups. Local richness showed statistically significant, season-dependent increases and decreases towards downstream location within the catchment for fish and bacteria, respectively. Meanwhile, invertebrate richness remained spatially unchanged but varied across seasons. The structure of local food webs, such as link density and nestedness, also varied across space and time. However, these patterns did not necessarily mirror those observed for biodiversity and functional feeding characteristics. Our results suggest that biodiversity patterns and food-web dynamics are not directly scalable to each other even at the same spatial and temporal scales. In order to conserve species diversity as well as the functional trophic integrity of communities, patterns of biodiversity and food-web characteristics must thus be jointly studied.