bluuebunny/crossref_metadata_2025 huggingface dataset:
PAC1 Agonist Maxadilan Reduces Atherosclerotic Lesions in Hypercholesterolemic ApoE-Deficient Mice
Authors: Mey Lilli , Bonaterra Gabriel A. , Hoffmann Joy , Schwarzbach Hans , Schwarz Anja , Eiden Lee E. , Weihe Eberhard , Kinscherf Ralf
Published: December 2024

A possible involvement of immune- and vasoregulatory PACAP signaling at the PAC1 receptor in atherogenesis and plaque-associated vascular inflammation has been suggested. Therefore, we tested the PAC1 receptor agonist Maxadilan and the PAC1 selective antagonist M65 on plaque development and lumen stenosis in the ApoE−/− atherosclerosis model for possible effects on atherogenesis. Adult male ApoE−/− mice were fed a cholesterol-enriched diet (CED) or standard chow (SC) treated with Maxadilan, M65 or Sham. Effects of treatment on atherosclerotic plaques, lumen stenosis, apoptosis and pro-inflammatory signatures were analyzed in the brachiocephalic trunk (BT). The percentage of Maxadilan treated mice exhibiting plaques under SC and CED was lower than that of Sham or M65 treatment indicating opposite effects of Maxadilan and M65. Maxadilan application inhibited lumen stenosis in SC and CED mice compared to the Sham mice. In spite of increased cholesterol levels, lumen stenosis of Maxadilan-treated mice was similar under CED and SC. In contrast, M65 under SC or CED did not reveal a significant influence on lumen stenosis. Maxadilan significantly reduced the TNF-α-immunoreactive (TNF-α+) area in the plaques under CED, but not under SC. In contrast, the IL-1β+ area was reduced after Maxadilan treatment in SC mice but remained unchanged in CED mice compared to Sham mice. Maxadilan reduced caspase-3 immunoreactive (caspase-3+) in the tunica media under both, SC and CED without affecting lipid content in plaques. Despite persistent hypercholesterolemia, Maxadilan reduces lumen stenosis, apoptosis and TNF-α driven inflammation. Our data suggest that Maxadilan provides atheroprotection by acting downstream of hypercholesterolemia-induced vascular inflammation. This implicates the potential of PAC1-specific agonist drugs against atherosclerosis even beyond statins and PCSK9 (proprotein convertase subtilisin/kexin type 9) inhibitors.
Extracellular PKM2 Preserves Cardiomyocytes and Reduces Cardiac Fibrosis During Myocardial Infarction
Authors: Huang Yang , Li Bin , Gui Zongxiang , Gao Erhe , Yuan Yi , Yang Jenny , Hekmatyar Khan , Mishra Falguni , Chan Payton , Liu Zhiren
Published: December 2024

Substantial loss of cardiomyocytes during heart attacks and onset of other cardiovascular diseases is a major cause of mortality. Preservation of cardiomyocytes during cardiac injury would be the most effective strategy to manage these diseases in clinic. However, there is no effective treatment strategy that is able to prevent cardiomyocyte loss. We demonstrate here that the systemic administration of a recombinant PKM2 mutant (G415R) preserves cardiomyocytes and reduces cardiac fibrosis during myocardial infarction. G415R preserves cardiomyocytes by protecting the cardiomyocytes from dying and by promoting cardiomyocyte proliferation. Preservation of cardiomyocytes by extracellular PKM2 (EcPKM2) reduces cardiac fibrosis because of the decreased activation of cardiac fibroblasts. Our experiments show that EcPKM2 (G415R) exerts its action by interacting with integrin avb3 on cardiomyocytes. EcPKM2(G415R) activates the integrin–FAK–PI3K signaling axis, which subsequently suppresses PTEN expression and consequently regulates cardiomyocyte apoptosis resistance and proliferation under hypoxia and oxidative stress conditions. Our studies uncover an important cardiomyocyte protection mechanism. More importantly, the activity/action of EcPKM2 (G415R) in preserving cardiomyocyte suggesting a possible therapeutic strategy and target for the treatment of heart attacks and other cardiovascular diseases.
Challenges and Future Directions in Lipoprotein Measurement for Atherosclerosis Prevention and Treatment
Authors: Konishi Tomokazu
Published: December 2024

Atherosclerosis can cause severe damage to the heart, brain, and other vital organs [...]
SLC7A11 Expression Is Up-Regulated in HPV- and Tobacco-Associated Lung Cancer
Authors: Osorio Julio C. , Andrade-Madrigal Cristian , Gheit Tarik , Corvalán Alejandro H. , Aguayo Francisco
Published: December 2024

High-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical, anogenital, and a subset of oropharyngeal cancers. In addition, HR-HPVs have been detected in lung carcinomas worldwide, even though the role of these viruses in this type of cancer is not fully understood. This study evaluated the presence of HPV in a cohort of 204 lung cancer cases by multiplex polymerase chain reaction (PCR)-Luminex. In addition, we used transcriptomic approaches to characterize the HPV-associated gene expression profile in the context of tobacco-smoke-associated lung cancer. HPV16 was detected in 8/204 lung carcinomas (4.0%). Through a significance analysis of microarrays (SAM) analysis, we found that the solute carrier family 7-member 11 (SLC7A11/xCT) gene (an antiporter that mediates the uptake of extracellular cystine) is up-regulated in tobacco-smoke- and HPV-associated lung cancers. In addition, SLC7A11 up-regulation correlates with both HR-HPV16 E6/E7 expression and tobacco smoke exposure in lung epithelial cells. Furthermore, we found decreased survival in HPV/SLC7A11-positive patients with lung cancer when compared to HPV/SLC7A11-negative cases. Thus, this study suggests that SLC7A11 up-regulation is associated with both HPV-positive and tobacco-smoke-associated lung carcinomas, with a potential association with clinical prognosis.
Young Goji Fruit Volatiles Regulate the Oviposition Behavior and Chemosensory Gene Expression of Gravid Female Neoceratitis asiatica
Authors: Wei Hongshuang , Liu Kexin , Zhang Jingyi , Guo Kun , Liu Sai , Xu Changqing , Qiao Haili , Tan Shuqian
Published: December 2024

The goji fruit fly, Neoceratitis asiatica, is a major pest on the well-known medicinal plant Lycium barbarum. Dissecting the molecular mechanisms of the oviposition selection of N. asiatica regarding the host plant will help to identify new strategies for pest fly control. However, the molecular mechanism of chemical communication between the goji fruit fly and the host goji remains unclear. Hence, our study found that young goji fruit volatiles induced the oviposition response of gravid female N. asiatica. After N. asiatica was exposed to young goji fruit volatiles, the expression of six chemosensory genes (NasiOBP56h3 and OBP99a1 in the antennae; OBP99a2, OBP99a3 and CSP2 in the legs; and OBP56a in the ovipositor) was significantly upregulated in different organs of female N. asiatica compared with the group without odor treatment according to transcriptome data. Further results of qPCR verification show that the expression levels of the six selected upregulated genes after the flies were exposed to host plant volatiles were mostly consistent with the results of transcriptome data. We concluded that six upregulated genes may be involved in the recognition of young goji fruit volatiles by gravid female N. asiatica. Our study preliminarily identifies young goji fruit volatiles as a key factor in the oviposition behavior of N. asiatica, which will facilitate further studies on the mechanisms of host oviposition selection in N. asiatica.
Enhancing Clinical Applications by Evaluation of Sensitivity and Specificity in Whole Exome Sequencing
Authors: Moon Youngbeen , Hong Chung Hwan , Kim Young-Ho , Kim Jong-Kwang , Ye Seo-Hyeon , Kang Eun-Kyung , Choi Hye Won , Cho Hyeri , Choi Hana , Lee Dong-eun , Choi Yongdoo , Kim Tae-Min , Heo Seong Gu , Han Namshik , Hong Kyeong-Man
Published: December 2024

The cost-effectiveness of whole exome sequencing (WES) remains controversial due to variant call variability, necessitating sensitivity and specificity evaluation. WES was performed by three companies (AA, BB, and CC) using reference standards composed of DNA from hydatidiform mole and individual blood at various ratios. Sensitivity was assessed by the detection rate of null–homozygote (N–H) alleles at expected variant allelic fractions, while false positive (FP) errors were counted for unexpected alleles. Sensitivity was approximately 20% for in-house results from BB and CC and around 5% for AA. Dynamic Read Analysis for GENomics (DRAGEN) analyses identified 1.34 to 1.71 times more variants, detecting over 96% of in-house variants, with sensitivity for common variants increasing to 5%. In-house FP errors varied significantly among companies (up to 13.97 times), while DRAGEN minimized this variation. Despite DRAGEN showing higher FP errors for BB and CC, the increased sensitivity highlights the importance of effective bioinformatic conditions. We also assessed the potential effects of target enrichment and proposed optimal cutoff values for the read depth and variant allele fraction in WES. Optimizing bioinformatic analysis based on sensitivity and specificity from reference standards can enhance variant detection and improve the clinical utility of WES.
DEAD/H Box 5 (DDX5) Augments E2F1-Induced Cell Death Independent of the Tumor Suppressor p53
Authors: Nakajima Rinka , Zhou Yaxuan , Shirasawa Mashiro , Nishimura Naoyasu , Zhao Lin , Fikriyanti Mariana , Kamiya Yuki , Iwanaga Ritsuko , Bradford Andrew P. , Shinmyozu Kaori , Nishibuchi Gohei , Nakayama Jun-ichi , Kurayoshi Kenta , Araki Keigo , Ohtani Kiyoshi
Published: December 2024

In almost all cancers, the p53 pathway is disabled and cancer cells survive. Hence, it is crucially important to induce cell death independent of p53 in the treatment of cancers. The transcription factor E2F1 is controlled by binding of the tumor suppressor pRB, and induces apoptosis by activating the ARF gene, an upstream activator of p53, when deregulated from pRB by loss of pRB function. Deregulated E2F1 can also induce apoptosis, independent of p53, via other targets such as TAp73 and BIM. We searched for novel E2F1-interacting proteins and identified the RNA helicase DEAD/H box 5 (DDX5), which also functions as a transcriptional coactivator. In contrast to the reported growth-promoting roles of DDX5, we show that DDX5 suppresses cell growth and survival by augmentation of deregulated E2F1 activity. Over-expression of DDX5 enhanced E2F1 induction of tumor suppressor gene expression and cell death. Conversely, shRNA-mediated knockdown of DDX5 compromised both. Moreover, DDX5 modulated E2F1-mediated cell death independent of p53, for which DDX5 also functions as a coactivator. Since p53 function is disabled in almost all cancers, these results underscore the roles of DDX5 in E2F1-mediated induction of cell death, independent of p53, and represent novel aspects for the treatment of p53-disabled cancer cells.
Research Progress and Prospects of Molecular Breeding in Bermudagrass (Cynodon dactylon)
Authors: Sun Xiaoyang , Fu Qiang , Song Yuxiao , Deng Xinjie , Li Yinruizhi , Wu Ke , Li Shuning , Fu Jinmin
Published: December 2024

Bermudagrass (Cynodon dactylon L.) is a warm-season grass species of significant ecological and economic importance. It is widely utilized in turf management and forage production due to its resilience to drought, salt, and other environmental stresses. Recent advancements in molecular breeding, particularly through genomics technology and gene editing, have enabled the efficient identification of key genes associated with stress tolerance and turf quality. The use of techniques such as overexpression and CRISPR/Cas has enhanced resistance to drought, salt, cold, and heat, while the application of molecular markers has accelerated the development of superior varieties. The integration of multi-omics, such as genomics, transcriptomics, and proteomics, provides deeper insights into the molecular mechanisms of bermudagrass, thereby improving breeding efficiency and precision. Additionally, artificial intelligence is emerging as a powerful tool for analyzing genomic data, predicting optimal trait combinations, and accelerating breeding processes. These technologies, when combined with traditional breeding methods, hold great potential for optimizing bermudagrass varieties for both turf and forage use. Future research will focus on further integrating these tools to address the challenges of breeding posed by climate change to breeding climate-resilient turf and forage crops.
Single-Cell and Spatial Multi-Omics Analysis Reveal That Targeting JAG1 in Epithelial Cells Reduces Periodontal Inflammation and Alveolar Bone Loss
Authors: Kuang Shuhong , Yang Jiayu , Shen Zongshan , Xia Juan , Lin Zhengmei
Published: December 2024

Mucosal immunity plays a critical role in the pathogenesis of inflammatory immune diseases. This study leverages single-cell RNA sequencing, spatial transcriptomics, and spatial proteomics to compare the cellular mechanisms involved in periodontitis between humans and mice, aiming to develop precise strategies to protect the gingival mucosal barrier. We identified key conserved and divergent features in cellular landscapes and transcriptional profiles across the two species, underscoring the complexity of inflammatory responses and immune dynamics in periodontitis. Additionally, we revealed a novel regulatory mechanism by which epithelial cells modulate macrophage behavior and inflammation through the JAG1–Notch pathway. Validation through animal experiments revealed that JAG1 inhibition reduces inflammation in epithelial cells, mitigating periodontitis. Our findings advance the understanding of periodontal disease pathogenesis and highlight the importance of integrating human and animal model data to develop treatments aligned with human physiology, offering potential therapeutic targets for controlling inflammation and enhancing tissue regeneration.
Alterations in the Rice Coleoptile Metabolome During Elongation Under Submergence Stress
Authors: Yemelyanov Vladislav V. , Puzanskiy Roman K. , Bogdanova Ekaterina M. , Vanisov Sergey A. , Kirpichnikova Anastasia A. , Biktasheva Maria O. , Mukhina Zhanna M. , Shavarda Alexey L. , Shishova Maria F.
Published: December 2024

Plants known as obligate aerobes developed different mechanisms to overcome the damage incurred under oxygen limitation. One of the survival strategies to have commonly appeared in hydrophytic plants is the escape strategy, which accelerates plant axial organs’ growth in order to escape hypoxic conditions as soon as possible. The present study aimed to distinguish the alterations in coleoptile elongation, viability and metabolic profiles in coleoptiles of slow- and fast-growing rice varieties. All the parameters were tested at 3, 5 and 7 days after sowing, to highlight changes during seedling development in normal and submerged conditions. The obtained results indicated that coleoptile elongation correlated with higher resistance to oxygen deprivation. GS-MS-based metabolic profiling indicated that coleoptiles of the fast-growing cultivar accumulated higher amounts of sugar phosphates, disaccharides, fatty acid derivatives and sterols, which are important for maintaining growth, membrane stability and viability. The slow-growing variety was characterized by a greater abundance of carboxylates, including lactate and phosphoric acid, indicating an energy crisis and cytosol acidification, leading to cell damage and low tolerance. Therefore, a metabolomics approach could be used for phenotyping (chemotyping) in the large-scale screening of newly developed varieties with higher tolerance to oxygen deprivation.
Genome-Wide Identification of the Trihelix Transcription Factor Family and Functional Analysis of ZmTHX15 in Maize
Authors: Cao Yanyong , Cheng Zeqiang , Sun Xinyan , Zhu Meichen , Yue Ling , Liu Hui , Wu Xiaolin , Zhang Jinghua , Duan Canxing
Published: December 2024

The trihelix transcription factor, which is a plant-specific family, play a critical role in plant growth and development and stress responses. Drought is the main limiting factor affecting yield of maize (Zea mays). However, the identification and characterization of this gene family in maize and its biological functions in response to drought stress have not been reported. Here, 46 Zea mays trihelix genes (ZmTHXs) were identified in the genome. Phylogenetic analysis of the ZmTHXs revealed that the genes were clustered into five subfamilies: GT-1, GT-2, GTγ, SH4, and SIP1. Chromosomal localization analysis showed that the 46 ZmTHXs were unevenly distributed across 10 chromosomes in maize. Cis-acting elements related to abiotic stress in ZmTHXs were found. Most ZmTHXs genes showed significant changes in expression levels under drought treatment. In addition, ZmTHX15-overexpressing Arabidopsis exhibited stronger drought tolerance with less secondary oxidative damage and higher photosynthetic rate. These findings could serve as a basis for future studies on the roles of ZmTHXs and the potential genetic markers for breeding stress-resistant and high-yielding maize varieties.
Ion Channels in Odor Information Processing of Neural Circuits of the Vertebrate Olfactory Bulb
Authors: Yu Yunqing , Liao Ping , Jiang Ruotian
Published: December 2024

Olfactory disorders and their associated complications present a considerable challenge to an individual’s quality of life and emotional wellbeing. The current range of treatments, including surgical procedures, pharmacological interventions, and behavioral training, frequently proves ineffective in restoring olfactory function. The olfactory bulb (OB) is essential for odor processing and plays a pivotal role in the development of these disorders. Despite the acknowledged significance of ion channels in sensory functions and related pathologies, their specific involvement in OB remains unexplored. This review presents an overview of the functions of various ion channel families in regulating neuronal excitability, synaptic transmission, and the complex processes of olfactory perception. The objective of this review was to elucidate the role of ion channels in olfactory function, providing new insights into the diagnosis and treatment of olfactory dysfunction.
Biological Function Analysis of MicroRNAs and Proteins in the Cerebrospinal Fluid of Patients with Parkinson’s Disease
Authors: Hwang Ji Su , Kim Seok Gi , George Nimisha Pradeep , Kwon Minjun , Jang Yong Eun , Lee Sang Seop , Lee Gwang
Published: December 2024

Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by alpha-synuclein aggregation into Lewy bodies in the neurons. Cerebrospinal fluid (CSF) is considered the most suited source for investigating PD pathogenesis and identifying biomarkers. While microRNA (miRNA) profiling can aid in the investigation of post-transcriptional regulation in neurodegenerative diseases, information on miRNAs in the CSF of patients with PD remains limited. This review combines miRNA analysis with proteomic profiling to explore the collective impact of CSF miRNAs on the neurodegenerative mechanisms in PD. We constructed separate networks for altered miRNAs and proteomes using a bioinformatics method. Altered miRNAs were poorly linked to biological functions owing to limited information; however, changes in protein expression were strongly associated with biological functions. Subsequently, the networks were integrated for further analysis. In silico prediction from the integrated network revealed relationships between miRNAs and proteins, highlighting increased reactive oxygen species generation, neuronal loss, and neurodegeneration and suppressed ATP synthesis, mitochondrial function, and neurotransmitter release in PD. The approach suggests the potential of miRNAs as biomarkers for critical mechanisms underlying PD. The combined strategy could enhance our understanding of the complex biochemical networks of miRNAs in PD and support the development of diagnostic and therapeutic strategies for precision medicine.
Comparative Transcriptome Analysis Revealing the Potential Salt Tolerance Mechanism of Exogenous Abscisic Acid Application in Melilotus albus
Authors: Chen Lijun , Wu Fan , Duan Zhen , Wang Shengsheng , Qu Yuncan , Ao Bao , Sun Xiaojuan , Zhang Jiyu
Published: December 2024

Melilotus albus, which contains abundant pharmacologically active coumarins, is usually used as a rotation crop and green manure worldwide. Abscisic acid (ABA) is a crucial plant hormone that plays an important role in plant stress responses. There is a paucity of information about the ABA signaling pathway and its regulatory network in M. albus. Here, we performed a comparative physiological and transcriptomic analysis to assess the response of M. albus to exogenous ABA. Physiological analysis revealed that proline (Pro), soluble protein and H2O2 content after ABA treatment 3 h significantly increased by 14.0%, 12.0% and 32.4% compared with 0 h in M. albus. A total of 19,855 differentially expressed genes (DEGs) were identified under ABA treatment, including 13,392 in shoots and 15,471 in roots. We obtained two modules that were significantly correlated with the ABA treatment (the darkorange module was positively correlated at 24 h in the shoot, brown2 module positively correlated at 3 h in the root) by weighted correlation network analysis (WGCNA). KEGG enrichment analysis showed that genes within two modules were primarily enriched in protein synthesis and metabolism, secondary metabolites, purine and pyrimidine metabolism, and phenylalanine, tyrosine and tryptophan biosynthesis. GO enrichment analysis indicated that genes within two modules were primarily enriched in energy substance metabolism. These pathways were mainly associated with abiothic stress, which indicated that exogenous application of ABA activated the stress resistance system of M. albus. The hub gene 4CL1 (4-Coumarate: CoA ligase 1) was translated and expressed in yeast, resulting in enhanced salt and ABA tolerance in the transgenic yeast. Overexpression of Ma4CL1 in M. albus improved the salt resistance of the transgenic plants. Profiling ABA-responsive genes offers valuable insights into the molecular functions of regulatory genes and will facilitate future molecular breeding efforts in M. albus.
Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture
Authors: Sun Zhidan , Ding Chenglong , Wang Yuhan , Lu Tingting , Song Wencheng
Published: December 2024

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) is an innovative cancer treatment method that has received considerable scientific attention. The objective of this study is to evaluate the effects of PAM on the anti-tumor characteristics of non-small cell lung cancer (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures. The effects of PAM treatment on the proliferative and migratory capabilities of A549 cells in 2D and 3D cultures were assessed using MTT, migration, invasion assays, and cell cycle, respectively. The study also investigated the impact of PAM treatment on the changes in the content of intracellular and extracellular reactive species and analyzed protein expression using the Western Blot method. PAM treatment inhibited the viability, migration, and invasion abilities of A549 cells in both 2D and 3D cultures, suppressed the epithelial–mesenchymal transition (EMT) process, and downregulated the expression of the RAS/ERK signaling pathway, which effectively inhibited tumor spheroid formation. Additionally, the effect of PAM on A549 cells was mediated through ROS-induced oxidative reactions, and PAM treatment exhibited greater cytotoxicity in 2D culture compared to 3D culture. As compared to 2D, the 3D cell culture model provides a viable in vitro cell model for studying the mechanisms of PAM treatment in lung cancer. PAM represents an effective new treatment for NSCLC.
Synergistic Effects of the Combination of Alpelisib (PI3K Inhibitor) and Ribociclib (CDK4/6 Inhibitor) in Preclinical Colorectal Cancer Models
Authors: Aslam Razia , Richards Cathy E. , Fay Joanna , Hudson Lance , Workman Julie , Lee Cha Len , Murphy Adrian , O’Neill Brian , Toomey Sinead , Hennessy Bryan T.
Published: December 2024

The CDK4/6 inhibitor Ribociclib has shown limited efficacy as a monotherapy in colorectal cancer (CRC). However, combining Ribociclib with targeted therapies could present a viable strategy for treating CRC. This study evaluated the combination of Ribociclib and the PI3K inhibitor Alpelisib across four distinct cell lines representing different mutational statuses (PIK3CA/KRAS wild-type, KRAS-mutated, PIK3CA-mutated, and PIK3CA/KRAS-mutated). We analyzed the drugs’ impact on key proteins involved in the PI3K pathway, cell cycle regulation, and apoptosis. The combination of Alpelisib and Ribociclib demonstrated a synergistic anti-proliferative effect across all cell lines, leading to a simultaneous decrease in pRB, pAKT, and p-S6 levels, and a more comprehensive suppression of the PI3K/AKT/mTOR pathway. Additionally, there was an upregulation of the apoptotic marker, p-BCL2, in cells treated with the combination compared to controls. In vivo studies using Caco-2, LS1034, and SNUC4 xenografts revealed a significant reduction in tumour growth with the combination therapy compared to single-agent treatments. These findings suggest that combining Alpelisib and Ribociclib could be a promising therapeutic approach for CRC, warranting further clinical exploration.
HMGA1 Plays a Role in Counteracting DNA Damage Induced by BoHV-1 Productive Infection
Authors: Zhao Heci , Fu Xiaotian , Ding Xiuyan , Zhu Liqian
Published: December 2024

Bovine herpesvirus 1 (BoHV-1) productive infection induces the generation of DNA double-strand breaks (DSBs), which may consequently lead to cell apoptosis. In response to DSBs, the DNA damage repair-related protein 53BP1 is recruited to the sites of DSBs, leading to the formation of 53BP1foci, which are crucial for the repair of damaged DNA and maintaining genomic integrity by repairing DSBs. In this study, we discovered that HMGA1 may play a significant role in counteracting virus infection-induced DNA damage, as the siRNA-mediated knockdown of HMGA1 protein expression or inhibition of HMGA1 activity by the chemical inhibitor Netropsin uniformly exacerbates the DNA damage induced by BoHV-1 productive infection. Interestingly, HMGA1 may positively regulate 53BP1 expression, and treatment with Netropsin reduced the accumulation of 53BP1 protein in the nucleus, suggesting that HMGA1 may potentially influence 53BP1’s nuclear localization. However, this effect was reversed in the context of virus infection. Furthermore, Netropsin treatment restored the disruption of 53BP1 foci caused by virus infection, which is consistent with our findings that Netropsin enhances the nuclear accumulation of 53BP1. Collectively, these results indicate that HMGA1 is involved in countering DNA damage induced by virus infection. HMGA1 does indeed modulate the nuclear accumulation of 53BP1 protein, but this effect is counteracted by virus infection. Therefore, the biological function of HMGA1 in countering virus infection-induced DNA damage may be independent of its regulation of 53BP1 signaling. This is the first report suggesting that HMGA1 may be implicated in virus infection-induced DNA damage, although the precise mechanism remains to be elucidated. Furthermore, we report for the first time an interaction between HMGA1 and 53BP1, which is disrupted following virus infection.
A Novel TiO2-Cuttlebone Photocatalyst for Highly Efficient Catalytic Degradation of Tetracycline Hydrochloride
Authors: Li Qing , Liu Penghui , Lin Huizhen , Xue Hun , Mao Jingyun
Published: December 2024

The harmful effects of antibiotics on aquatic environments have become a growing concern of modern society. Developing high-performance photocatalysts capable of degrading antibiotics under solar light is, therefore, crucial. In this study, TiO2-cuttlebone composites are prepared via the sol–gel method, to produce carbonate radicals (•CO3−) under solar light irradiation. The •CO3− radicals exhibit high selectivity for the degradation of tetracycline hydrochloride (TC). Compared to TiO2 alone, the TiO2-cuttlebone composite demonstrates excellent solar-driven photocatalytic activity for TC degradation in both freshwater and seawater. The reaction pathways of TC degradation in seawater are elucidated using HPLC-MS/MS analysis. Moreover, a TiO2-cuttlebone self-suspending photocatalyst device is fabricated using 3D printing technology and low-temperature deposition methods, with aluminum–plastic (AP) as a substrate. This innovative device is easily recyclable from photocatalytic solutions while maintaining high stability, making it highly desirable for practical applications.
Online Purchase Behavior Prediction Model Based on Recurrent Neural Network and Naive Bayes
Authors: Zhang Chaohui , Liu Jiyuan , Zhang Shichen
Published: December 2024

In the current competition process of e-commerce platforms, the technical and algorithmic wars that can quickly grasp user needs and accurately recommend target commodities are the core tools of platform competition. At the same time, the existing online purchase behavior prediction models lack consideration of time series features. This paper combines the Recurrent Neural Network, which is more suitable for the commodity recommendation scenario of the e-commerce platform, with Naive Bayes, which is simple in logic and efficient in operation and constructs the online purchase behavior prediction model RNN-NB, which can consider the features of time series. The RNN-NB model is trained and tested using 3 million time series data with purchase behavior provided by the Ali Tianchi big data platform. The prediction effect of the RNN-NB model and Naive Bayes model is evaluated and compared respectively under the same experimental conditions. The results show that the overall prediction effect of the RNN-NB model is better and more stable. In addition, through the analysis of user time series features, it can be found that the possibility of user purchase is negatively correlated with the length of time series, and merchants should pay more attention to those users with shorter time series in commodity recommendation and targeted offers. The contributions of this paper are as follows: (1) By constructing an online purchasing behavior model RNN-NB, which integrates the N vs 1 structure Recurrent Neural Network and naive Bayesian model, the validity limitations of some single-architecture recommendation algorithms are solved. (2) Based on the existing naive Bayesian model, the prediction accuracy of online purchasing behavior is further improved. (3) The analysis based on the features of the time series provides new ideas for the research of later scholars and new guidance for the marketing of platform merchants.
Synthesis of (5Z)-3-Allyl-5-{[5-(4-methoxyphenyl)thiophen-2-yl]methylidene}-2-sulfanylidene-1,3-thiazolidin-4-one in L-Proline-Based Deep Eutectic Solvent
Authors: Hesse Stéphanie , Jourdain Isabelle , Khatyr Abderrahim , Knorr Michael
Published: December 2024

3-N-allylrhodanine was condensed with 5-(4-methoxyphenyl)-thiophene-2-carbaldehyde in an L-proline-based deep eutectic solvent (DES) to obtain the π-conjugated heterocyclic rhodanine compound (5Z)-3-allyl-5-{[5-(4-methoxyphenyl)thiophen-2-yl]methylidene}-2-sulfanylidene-1,3-thiazolidin-4-one (2). Compound 2 was characterized by NMR spectroscopy, and its UV-vis spectrum was compared with that of the related derivative 3-allyl-5-(4-methoxybenzylidene)-2-sulfanylidene-1,3-thiazolidin-4-one (1). Preliminary results revealed that compound 2 is emissive at room temperature in solution.
Influence of Magnetic Stirring and Eutectic Front Velocity on the Solidified Microstructure of Al-18 wt.% Si Alloy
Authors: Zakaraia Dimah , Roósz András , Rónaföldi Arnold , Veres Zsolt
Published: December 2024

The microstructure of hypereutectic Al-Si alloys is crucial in determining their mechanical properties and overall performance in engineering applications. This paper investigates the effect of a rotating magnetic field (RMF) and eutectic front velocity on the microstructure of hypereutectic Al-18 wt.% Si alloy. The hypereutectic samples were solidified using five different front velocities (0.02, 0.05, 0.09, 0.2, and 0.4 mm/s) with an average temperature gradient (G) of 8 K/mm in a crystallizer equipped with an RMF inductor. The samples were solidified into two sections. The first section solidified without stirring, while the second section solidified with stirring using RMF at an induction (B) of 7.2 mT. The length, angular orientation of eutectic Si lamellas, and interlamellar distances were measured in both the non-stirred and the stirred sections to evaluate the impact of RMF and front velocity on the eutectic structure. The results revealed that the application of RMF and the increase in front velocity during solidification led to the significant refinement of the eutectic structure. These findings highlight the potential of RMF and front velocity manipulation to enhance the microstructure of hypereutectic Al-Si alloys, with practical implications for the development of high-performance materials.
Fabrication of ZrO2 Armor Ceramics by 3D Printing Accompanied with Microwave Sintering
Authors: Liang Zhengang , Zhang Dongjiang , Chen Xin , Pang Chunxu , Guo Xuncheng , Feng Yanfei , Xu Xiqing
Published: December 2024

Ceramic armor protection with complex shapes is limited by the difficult molding or machining processing, and 3D printing technology provides a feasible method for complex-shaped ceramics. In this study, ZrO2 ceramics were manufactured by 3D printing accompanied with microwave sintering. In 3D printing, the formula of photosensitive resin was optimized by controlling the content of polyurethane acrylic (PUA) as oligomer, and the photosensitive resin with 50% PUA showed excellent curing performance with a small volume shrinkage of 4.05%, media viscosity of 550 mPa·s, and low critical exposure of 20 mJ/cm2. Compared to conventional sintering, microwave sintering was beneficial to dense microstructures with fine grain size, and microwave sintering at 1500 °C was confirmed as an optimized sintering process for the 3D-printed ZrO2 ceramics, and the obtained ceramics showed a relative density of 98.2% and mean grain size of 2.1 μm. The PUA content further affected the microstructure and mechanical property of the ZrO2 ceramics. The sample with 10%~40% PUA showed some pores due to the low viscosity and large volume shrinkage of photosensitive resins, and the sample with 60% PUA exhibited an inhomogeneous microstructure with agglomeration, attributed to the high viscosity of photosensitive resins. Finally, the ZrO2 ceramics via 3D printing with 50% PUA showed superior mechanical properties, whose Vickers hardness was 3.4 GPa, fracture toughness was 7.4 MPa·m1/2, flexure strength was 1038 MPa, and dynamic strength at 1200 s−1 was 4.9 GPa, conducive to the material’s employment as armor protection ceramics.
Electret Nonwoven Structures for High-Efficiency Air Filtration, Produced Using the Blow Spinning Technique
Authors: Penconek Agata , Bąkała Natalia , Jackiewicz-Zagórska Anna , Małolepszy Artur , Przekop Rafał , Moskal Arkadiusz
Published: December 2024

This study explores the fabrication of electret nonwoven structures for high-efficiency air filtration, utilizing the blow spinning technique. In response to the growing need for effective filtration systems, we aimed to develop biodegradable materials capable of capturing fine particulate matter (PM2.5) without compromising environmental sustainability. Polylactic acid (PLA) was used as the primary polymer, with the addition of SiO2 and MoS2 to enhance the fibers’ charge retention and filtration performance. The fibers were charged electrostatically to improve particle capture efficiency. The experimental results showed that fibers containing 5% MoS2 exhibited the highest filtration efficiency, surpassing those with SiO2, despite MoS2 being a semiconductor and SiO2 a dielectric. Furthermore, the addition of MoS2 improved the filtration efficiency across a range of particle sizes (0.2–1 µm) while maintaining a manageable pressure drop. These findings suggest that incorporating MoS2 in electret nonwoven structures can significantly improve filtration performance, making it a promising material for advanced air filtration applications. This study contributes to the development of eco-friendly filtration materials with high performance, essential in reducing exposure to airborne pollutants.
Magnetic Properties of All-d Metallic Heusler Compounds: A First-Principles Study
Authors: Tas Murat , Şaşıoğlu Ersoy , Galanakis Iosif
Published: December 2024

All-d metallic Heusler compounds are promising materials for nanoelectronic applications. Such materials combining 3d, 4d, and 5d atoms have not yet been studied. In this respect, we perform ab initio electronic structure calculations and focus on Co2MnZ, Rh2MnZ, and Ru2MnZ compounds, where Z represents transition metal atoms from groups 3B, 4B, 5B, and 6B of the periodic table. Our results demonstrate that most of these compounds exhibit a distinctive region of very low minority-spin state density at the Fermi level when crystallized in the L21 lattice structure. The Co-based compounds follow a Slater–Pauling behavior for their total spin magnetic moments, while the Ru-based compounds consistently deviate from the predicted Slater–Pauling values. Rh-based compounds show similarities to Co-based compounds for lighter Z atoms and to Ru-based compounds for heavier Z atoms. We find that the choice of the Z element within the same periodic table column has only a minor effect on the results, except for the Rh2Mn(Cr, Mo, W) compounds. Our findings suggest that these compounds hold significant promise for applications in spintronics and magnetoelectronics.
Continual Semi-Supervised Malware Detection
Authors: Chin Matthew , Corizzo Roberto
Published: December 2024

Detecting malware has become extremely important with the increasing exposure of computational systems and mobile devices to online services. However, the rapidly evolving nature of malicious software makes this task particularly challenging. Despite the significant number of machine learning works for malware detection proposed in the last few years, limited interest has been devoted to continual learning approaches, which could allow models to showcase effective performance in challenging and dynamic scenarios while being computationally efficient. Moreover, most of the research works proposed thus far adopt a fully supervised setting, which relies on fully labelled data and appears to be impractical in a rapidly evolving malware landscape. In this paper, we address malware detection from a continual semi-supervised one-class learning perspective, which only requires normal/benign data and empowers models with a greater degree of flexibility, allowing them to detect multiple malware types with different morphology. Specifically, we assess the effectiveness of two replay strategies on anomaly detection models and analyze their performance in continual learning scenarios with three popular malware detection datasets (CIC-AndMal2017, CIC-MalMem-2022, and CIC-Evasive-PDFMal2022). Our evaluation shows that replay-based strategies can achieve competitive performance in terms of continual ROC-AUC with respect to the considered baselines and bring new perspectives and insights on this topic.
Two New Diterpenoids Formed by Transannular Diels–Alder Cycloaddition from the Soft Coral Sarcophyton tortuosum, and Their Antibacterial and PPAR-β Agonist Activities
Authors: Sun Min , Li Songwei , Zeng Jianang , Guo Yuewei , Wang Changyun , Su Mingzhi
Published: December 2024

Two new cembrane-derived tricyclic diterpenes belonging to the sarcophytin family, namely 4a-hydroxy-chatancin (1) and sarcotoroid (2), together with two known related ones (3 and 4), were isolated from the soft coral Sarcophyton tortuosum collected off Ximao Island in the South China Sea. The structures of the new compounds were elucidated by extensive spectroscopic analysis, a quantum mechanical nuclear magnetic resonance (QM-NMR) method, a time-dependent density functional theory electronic circular dichroism (TDDFT-ECD) calculation, X-ray diffraction analysis, and comparison with the reported data in the literature. A plausible biosynthetic pathway of compounds 1–4 was proposed, involving undergoing a transannular Diels–Alder cycloaddition. In the bioassay, the new compound 1 displayed significant inhibitory activities against the fish pathogens Streptococcus parauberis KSP28, oxytetracycline-resistant Streptococcus parauberis SPOF3K, and Photobacterium damselae FP2244, with MIC values of 9.1, 9.1, and 18.2 μg/mL, respectively. Furthermore, by conducting a luciferase reporter assay on rat liver Ac2F cells, compounds 1, 3, and 4 were evaluated for peroxisome proliferator-activated receptor (PPAR) transcriptional activity, and compound 3 showed selective PPAR-β agonist activity at a concentration of 10 μΜ.
Detection of Total Aflatoxins in Herbal Medicines Based on Lateral Flow Assay with Contamination Ratio Model
Authors: Qin Xiao-Ya , Feng Rui , Zhou Heng , Pan Hui-Qin , Wang Hao , Huang Xiao-Jing , Shen Jian-Ying , Hu Qing , Ji Shen
Published: December 2024

In this study, we developed a colloidal gold immunochromatographic strip (CGIS) method that used the matrix-matched calibration curves of contamination ratio models to quantitatively determine the total aflatoxin in five herbal medicines. This approach addresses issues related to false results and poor accuracy associated with conventional methods. The CGIS was analyzed using a Vertu touch reader, and the matrix-matched calibration was established based on the absorbance ratios of the T and C lines, as well as the logarithmic values of the total aflatoxin concentrations. The total aflatoxins could be accurately and digitally detected from 2.5 to 40 μg/kg, and the LOD of total aflatoxins was 1 μg/kg in the five herbal medicines. The recovery rates from the spiked samples ranged from 65.1% to 98.6%, and the RSD was less than 16.9%. A total of 229 samples were analyzed by both CGIS and HPLC-FLD, with agreement ranging from 78.4% to 132.6% (Arecae semen), 82.6% to 133.0% (Nelumbinis semen), 79.9% to 117.9% (Coicis semen), 78.1% to 119.0% (Platycladi semen), and 76.1% to 123.0% (Ziziphi spinosae semen). This process for the discrimination of the CGIS results was established to assess if samples met the requirement of aflatoxin limits, which could save approximately 75% in time and reduce the workload of retesting by a designated confirmatory reference method to less than 10%. This study demonstrated that the application of matrix-matched calibration curves based on contamination ratio models to CGIS can effectively enhance the rapid quantitative determination capability of total aflatoxins in herbal medicine matrices.
Relationship Between the Structure of the Flavone C-Glycosides of Linseed (Linum usitatissimum L.) and Their Antioxidant Activity
Authors: Ghozzi Imen , Fontaine Jean-Xavier , Molinié Roland , Elboutachfaiti Redouan , Akkouche Lylia , Sebei Khaled , Mathiron David , Hano Christophe , Garros Laurine , Choque Elodie , Roulard Romain , Petit Laurent , Delattre Cédric , Petit Emmanuel , Quéro Anthony
Published: December 2024

Flavonoids have been documented to have good antioxidant activities in vitro. In recent years, reports on the antioxidant activities of flavone C-glycosides, a subclass of flavonoids, have attracted great attention. Despite the wealth of information on this subject, the correlation between structure and function is not well understood. In this work, the relationship between the structure and the antioxidant activity of 12 flavone C-glycosides extracted from the aerial part of winter linseed (Linum usitatissimum L.) was studied to fill the current gaps. Orientin, isoorientin, vitexin, isovitexin, swertisin, swertiajaponin, carlinoside, schaftoside, lucenin-1, lucenin-2, vicenin-1, and vicenin-2 were purified by preparative HPLC and by the drowning-out crystallization method. Then, the control of the purity and the confirmation of the chemical structures were assessed by LC-MS and NMR analyses. The antioxidant activity was evaluated using ABTS, CUPRAC, FRAP, and iron chelating activity in vitro assays. Luteolin and its flavone C-glycoside derivatives exhibited higher antioxidant activity than apigenin and its flavone C-glycosides derivatives. This could be attributed to the ortho-dihydroxyl groups at C-3′ and C-4′ of the B ring in the flavonoid skeleton, which seemed to play an important role in antioxidant behavior. These results indicate that the antioxidant activity of these compounds, derived from apigenin and luteolin, can be closely related to their structural characteristics, including the position and nature of the sugars, the number of hydroxyl groups, and the presence of methyl group.
Seimatosporium chinense, a Novel Pestalotioid Fungus Associated with Yellow Rose Branch Canker Disease
Authors: Yang Haoran , Cheng Jing , Dili Nu , Jiang Ning , Ma Rong
Published: December 2024

Yellow rose (Rosa xanthina) is a common ornamental shrub species widely cultivated in China. However, canker disease symptoms were discovered during our investigations in Beijing and Xinjiang, China. The fungal isolates were obtained from diseased barks and identified using combined methods of morphology and phylogeny based on a partial region of ITS, LSU, rpb2, tef1, and tub2 sequences. As a result, a new species of Seimatosporium named S. chinense was proposed and described herein. The new species is distinguished from its phylogenetic sister species, S. gracile and S. nonappendiculatum, by conidial characters. The present study improves the species concept in Seimatosporium and provides fundamental data for the yellow rose canker disease control in the future.
Boronic Acid-Containing 3H- pyrazolo[4,3-f]quinoline Compounds as Dual CLK/ROCK Inhibitors with Anticancer Properties
Authors: Dayal Neetu , Chaudhuri Riddhi , Yeboah Kofi Simpa , Brauer Nickolas R. , Sintim Herman O.
Published: December 2024

Background: The protein kinases CLK and ROCK play key roles in cell growth and migration, respectively, and are potential anticancer targets. ROCK inhibitors have been approved by the FDA for various diseases and CLK inhibitors are currently being trialed in the clinic as anticancer agents. Compounds with polypharmacology are desired, especially in oncology, due to the potential for high efficacy as well as addressing resistance issues. In this report, we have identified and characterized novel, boron-containing dual CLK/ROCK inhibitors with promising anticancer properties. Methods: A library of boronic acid-based CLK/ROCKi was synthesized via Povarov/Doebner-type multicomponent reactions. Kinase inhibition screening and cancer cell viability assays were performed to identify the hit compounds. To gain insights into the probable binding modes of the compounds to the kinases, docking studies were performed. Cell cycle analysis, qPCR and immunoblotting were carried out to further characterize the mode(s) of action of the lead candidates. Results: At 25 nM, the top compounds HSD1400 and HSD1791 inhibited CLK1 and 2 and ROCK2 at greater than 70%. While HSD1400 also inhibited CLK4, the C1 methylated analog HSD1791 did not inhibit CLK4. Antitumor effects of the top compounds were evaluated and dose–response analysis indicated potent inhibition of renal cancer and leukemia cell growth. Immunoblotting results indicated that the top compounds induce DNA damage via upregulation of p-H2AX. Moreover, flow cytometry results demonstrated that the top compounds promote cell cycle arrest in the renal cancer cell line, Caki-1. qPCR and immunoblotting analysis upon HSD1791 dosing indicated suppression of cyclin D/Rb oncogenic pathway upon compound treatment. Conclusions: Novel boronic acid-containing pyrazolo[4,3-f]quinoline-based dual CLK/ROCK inhibitors were identified. The so-called “magic methylation” design approach was used to tune CLK selectivity. Additionally, the findings demonstrate potent in vitro anticancer activity of the lead candidates against renal cancer and leukemia. This adds to the growing list of boron-containing compounds that display biological activities.