bluuebunny/crossref_metadata_2025 huggingface dataset:
Movement patterns in Florida pine snakes are shaped by landscape heterogeneity, ambient air temperature and reproductive ethology
Authors: Orton Richard W , Wallrichs Megan , Bankovich Brittany , Bartolotti Glenn , Hayman Blair
Published: April 2023

Although variation in space use has been investigated extensively in terrestrial vertebrates, there have been recent criticisms that there are more data for mammals than reptiles. This may be an important consideration because the factors that shape movement patterns in reptiles could differ from those of mammals leading to misleading generalizations if data for mammals are extrapolated to reptiles. Here, we quantified the relationships between space use size, movement velocity and turn angle in 24 adult Florida pine snakes (Pituophis melanoleucus mugitus). We tested the effects of site, season, sex, the distribution of vegetation cover, and ambient air temperature on movement patterns. We also assessed the use of underground refugia across seasons, because underground refugia are thought to play an important role in the ecology and evolution of Florida pine snakes. Our results indicate that the movement patterns that shape space use variation in Florida pine snakes are affected by the heterogeneity of suitable microhabitats across landscapes, ambient air temperature and reproductive ethology. This study strengthens our collective understanding of movement patterns in Florida pine snakes and contributes to a growing body of knowledge of the movement ecology of snakes. Broadly, our results highlight general differences in the movement ecologies of mammals and Florida pine snakes that could potentially extend to our understanding of other ectothermic lineages, being predictably linked to metabolic theory.
Widespread coexistence of genetically distinct morphotypes in theSatyrium longicaudacomplex (Orchidaceae)
Authors: Castañeda-Zárate Miguel , Johnson Steven D , van der Niet Timotheüs
Published: April 2023

Species-level taxonomy is traditionally based on herbarium collections that typically include few, or even single, representatives per site. This can lead to underestimation of diversity when there are sympatric populations of superficially similar plants belonging to different lineages. Satyrium longicauda (Orchidaceae) represents a taxonomic challenge for the delimitation of species boundaries due to the high degree of morphological variation detected within and among populations. Currently, just two varieties are accepted based mainly on length differences of the lateral sepal and nectar spur. However, there is extensive morphological variation within South African populations and evidence for several pollination ecotypes, indicating that this taxon represents an actively diverging species complex. Here, we evaluate intraspecific morphological variation through uni- and multivariate morphometrics and analyse internal transcribed spacer sequences for individuals sampled from 36 sites, including 14 sites where divergent morphotypes occur sympatrically. Morphometric analyses of 1802 individuals revealed the presence of eight morphotypes based on vegetative and floral characters. Up to six morphologically and genetically distinct morphotypes can coexist in sympatry. Morphological and genetic distances among populations were significantly correlated. Phylogenetic analyses of 120 accessions indicated that neither of the two varieties nor S. longicauda as a species is monophyletic, and provided evidence for the monophyly of some of the morphotypes including the newly described S. cernuiflorum. The presence of distinct morphological and genetic sympatric variants, which in several cases scale up to distinct evolutionary lineages, is consistent with the existence of different taxa according to morphological and biological species concepts. Our results therefore confirm that taxonomy based mainly on herbarium collections can grossly under-estimate actual diversity of disparate lineages, although further work is required to finalize taxonomic decisions. These findings have implications for efforts to estimate species diversity in groups that are in the process of diversifying and for conservation practice.
Human stem cell-derived ventral midbrain astrocytes exhibit a region-specific secretory profile
Authors: Crompton Lucy A , McComish Sarah F , Steward Tom G J , Whitcomb Daniel J , Lane Jon D , Caldwell Maeve A
Published: April 2023

This scientific commentary refers to ‘Human stem cell-derived astrocytes exhibit region-specific heterogeneity in their secretory profiles’, by Clarke et al. (https://doi.org/10.1093/brain/awaa258) in Brain.
Role and Impact of a Clinical Pharmacy Team at an Inflammatory Bowel Disease Center
Authors: Choi David K , Rubin David T , Puangampai Archariya , Lach Monika
Published: April 2023

Background

There is limited literature describing the role of a clinical pharmacy team within a tertiary academic inflammatory bowel disease (IBD) center. The goal of this paper is to describe and showcase the clinical and operational impact of an integrated clinical pharmacy team.

Methods

This was a retrospective study evaluating the referral outcomes for all patients referred to University of Chicago Medicine Specialty Pharmacy for self-administered advanced IBD therapies covered by prescription insurance from October 1, 2020 to October 31, 2021.

Results

A total of 1800 referrals were received for advanced IBD therapies. Prior authorizations (PAs) were required and submitted for 1700 referrals. Of those 1700 PA submissions, 297 (17%) were denied by insurance. To overturn the denials, 344 appeals, including second-level appeals and external reviews, were submitted. Manufacturer patient assistance programs were obtained for 69 patients. From the 1800 referrals, 98% of patients were successfully started on the intended therapy. Clinically, there were 2141 pharmacist-initiated interventions by 2 IBD pharmacists. The most common interventions were prevention in interruption of therapy and providing patient education.

Conclusions

Clinical pharmacy teams are well positioned to streamline care within a tertiary academic IBD center. Their unique skillset and ability to provide high yield medication access supports the use of this model as a best practice in IBD centers.
Effects of supplemental nutrients on ovarian development and oviposition in Conogethes punctiferalis (Lepidoptera: Crambidae)
Authors: Zhang Ganyu , Wu Haibin , Jiang Lili , Wen Junbao , Zhang Wensheng , Sun Ruihong
Published: April 2023

Conogethes punctiferalis is a serious pest in China affecting a wide variety of field crops, fruits, and forest trees. Many insects require supplemental nutrients after emergence to compensate for insufficient nutrients at the larval stage. In this study, to better understand the determinants of C. punctiferalis survival and reproduction, the impact of supplemental nutrition was examined. In particular, the effects of 11 treatments (5% sucrose, 10% sucrose, 15% sucrose, 5% sophora honey, 10% sophora honey, 15% sophora honey, 5% wild honey, 10% wild honey, 15% wild honey, distilled water, and blank control) on adult longevity and oviposition were evaluated, with detailed morphological analyses of ovarian development in the 10% sucrose, distilled water, and blank control groups. Conogethes punctiferalis required supplementary nutrition after emergence. Supplementary nutrition improved fecundity (number of eggs laid) and longevity, and 10% sucrose water had the greatest effects. The preoviposition period and oviposition period of C. punctiferalis females were longer, and the numbers of eggs in female ovarian tubes and eggs laid by females were higher in the 10% sucrose water group than in other groups. Females supplemented with distilled water laid a small number of eggs. Without nutrient supplementation, females did not lay eggs. In conclusion, supplemental nutrition was beneficial for ovarian development in female moths, prolonging the oviposition period and lifespan, and was an important factor affecting population dynamics. These results lay a foundation for further analyses of the nutritional requirements for C. punctiferalis in the field and provide a reference for indoor population feeding.
Rice Farming and the Origins of Cooperative Behaviour
Authors: Zhou Xiaoyu , Alysandratos Theodore , Naef Michael
Published: April 2023

This paper provides novel evidence for links between historic farming practices and current norms of cooperative behaviour. We hypothesise that the cooperation required in wetland rice farming gives rise to strong cultural norms of cooperativeness. We compare participants from prefecture cities that predominately practice wetland rice cultivation to those from non-rice regions. A public goods game with and without punishment is the main measure for cooperative behaviour. Results indicate a strong and robust positive effect of wetland rice farming on cooperative behaviour and pro-social punishment. Complementary, consistent evidence from a natural field experiment and a survey further enriches our data.
What buffered the impact of the COVID-19 pandemic on depression? A longitudinal study of caregivers of school aged children in Ireland
Authors: Laurence James , Russell Helen , Smyth Emer
Published: April 2023

The COVID-19 pandemic has wrought acute harm to global mental health, especially among vulnerable populations. We explore what factors in people’s lives buffered the impact of the pandemic on depression; in particular, the role of social resources, economic resources, religiosity, and quality of their local environment. Drawing on three waves of longitudinal cohort data (two pre-pandemic waves and one pandemic-period wave) from primary caregivers of school-aged children in Ireland, we demonstrate that symptoms of depression increased sharply during the pandemic. However, depression symptomology increased less steeply among caregivers who, pre-pandemic, had greater economic resources and lived in higher quality environments, but especially among those with greater social resources and those who exhibited greater religiosity. Path analysis suggests that different sources of buffering might mitigate harm via different pathways. While most buffering factors appear to cushion mental well-being by reducing stresses from increased care work, improving familial relations, and helping caregivers manage the closure of/return to schools, other drivers appear to cushion mental well-being by reducing health anxieties around COVID-19, increasing opportunities for outdoor exercise, and protecting household incomes. This study highlights how crisis-preparedness should invest in social infrastructure alongside medical infrastructure to protect societies from future pandemics.
Impact of early vs. delayed atrial fibrillation catheter ablation on atrial arrhythmia recurrences
Authors: Kalman Jonathan M , Al-Kaisey Ahmed M , Parameswaran Ramanathan , Hawson Joshua , Anderson Robert D , Lim Michael , Chieng David , Joseph Stephen A , McLellan Alex , Morton Joseph B , Sparks Paul B , Lee Geoffrey , Sanders Prashanthan , Kistler Peter M
Published: April 2023

Background

Catheter ablation is an effective strategy in atrial fibrillation (AF). However, its timing in the course of management remains unclear. The aim of this study was to determine if an early vs. delayed AF ablation strategy is associated with differences in arrhythmia outcomes during 12-month follow-up.

Methods and results

One hundred patients with symptomatic AF referred to a tertiary centre for management were randomized in a 1:1 ratio to either an early ablation strategy (within 1 month of recruitment) or a delayed ablation strategy (optimized medical therapy followed by catheter ablation at 12 months post recruitment). The primary endpoint was atrial arrhythmia free survival at 12 months post-ablation. Secondary outcomes included: (i) AF burden, (ii) AF burden by AF phenotype, and (iii) antiarrhythmic drug (AAD) use at 12 months. Overall, 89 patients completed the study protocol (Early vs. Delayed: 48 vs. 41). Mean age was 59 ± 12.9 years (29% women). Pulmonary vein isolation was achieved in 100% of patients. At 12 months, 56.3% of patients in the early ablation group were free from recurrent arrhythmia, compared with 58.6% in the delayed ablation group (HR 1.12, 95% CI 0.59–2.13, P = 0.7). All secondary outcomes showed no significant difference including median AF burden (Early vs. Delayed: 0% [IQR 3.2] vs. 0% [5], P = 0.66), median AF burden amongst paroxysmal AF patients (0% [IQR 1.1] vs. 0% [4.5], P = 0.78), or persistent AF patients (0% [IQR 22.8] vs. 0% [5.6], P = 0.45) or AAD use (33% vs. 37%, P = 0.8).

Conclusion

Compared with an early ablation strategy, delaying AF ablation by 12 months for AAD management did not result in reduced ablation efficacy.
EHRA clinical consensus statement on conduction system pacing implantation: endorsed by the Asia Pacific Heart Rhythm Society (APHRS), Canadian Heart Rhythm Society (CHRS), and Latin American Heart Rhythm Society (LAHRS)
Authors: Burri Haran , Jastrzebski Marek , Cano Óscar , Čurila Karol , de Pooter Jan , Huang Weijian , Israel Carsten , Joza Jacqueline , Romero Jorge , Vernooy Kevin , Vijayaraman Pugazhendhi , Whinnett Zachary , Zanon Francesco
Published: April 2023

Conduction system pacing (CSP) has emerged as a more physiological alternative to right ventricular pacing and is also being used in selected cases for cardiac resynchronization therapy. His bundle pacing was first introduced over two decades ago and its use has risen over the last five years with the advent of tools which have facilitated implantation. Left bundle branch area pacing is more recent but its adoption is growing fast due to a wider target area and excellent electrical parameters. Nevertheless, as with any intervention, proper technique is a prerequisite for safe and effective delivery of therapy. This document aims to standardize the procedure and to provide a framework for physicians who wish to start CSP implantation, or who wish to improve their technique.
EHRA clinical consensus statement on conduction system pacing implantation: executive summary. Endorsed by the Asia-Pacific Heart Rhythm Society (APHRS), Canadian Heart Rhythm Society (CHRS) and Latin-American Heart Rhythm Society (LAHRS)
Authors: Burri Haran , Jastrzebski Marek , Cano Óscar , Čurila Karol , de Pooter Jan , Huang Weijian , Israel Carsten , Joza Jacqueline , Romero Jorge , Vernooy Kevin , Vijayaraman Pugazhendhi , Whinnett Zachary , Zanon Francesco
Published: April 2023

Conduction system pacing (CSP) has emerged as a more physiological alternative to right ventricular pacing and is also being used in selected cases for cardiac resynchronization therapy. His bundle pacing was first introduced over two decades ago and its use has risen over the last years with the advent of tools which have facilitated implantation. Left bundle branch area pacing is more recent but its adoption is growing fast due to a wider target area and excellent electrical parameters. Nevertheless, as with any intervention, proper technique is a prerequisite for safe and effective delivery of therapy. This document aims to standardize the procedure and to provide a framework for physicians who wish to start CSP implantation, or who wish to improve their technique. A synopsis is provided in this print edition of EP-Europace. The full document may be consulted online, and a ‘Key Messages’ App can be downloaded from the EHRA website.
Genetic testing in children with Brugada syndrome: results from a large prospective registry
Authors: Pannone Luigi , Bisignani Antonio , Osei Randy , Gauthey Anaïs , Sorgente Antonio , Vergara Pasquale , Monaco Cinzia , Della Rocca Domenico Giovanni , Del Monte Alvise , Strazdas Antanas , Mojica Joerelle , Al Housari Maysam , Miraglia Vincenzo , Mouram Sahar , Paparella Gaetano , Ramak Robbert , Overeinder Ingrid , Bala Gezim , Almorad Alexandre , Ströker Erwin , Pappaert Gudrun , Sieira Juan , de Ravel Thomy , La Meir Mark , Brugada Pedro , Chierchia Gian Battista , Van Dooren Sonia , de Asmundis Carlo
Published: April 2023

Aims

A pathogenic/likely pathogenic (P/LP) variant in SCN5A is found in 20–25% of patients with Brugada syndrome (BrS). However, the diagnostic yield and prognosis of gene panel testing in paediatric BrS is unclear. The aim of this study is to define the diagnostic yield and outcomes of SCN5A gene testing with ACMG variant classification in paediatric BrS patients compared with adults.

Methods and results

All consecutive patients diagnosed with BrS, between 1992 and 2022, were prospectively enrolled in the UZ Brussel BrS registry. Inclusion criteria were: (i) BrS diagnosis; (ii) genetic analysis performed with a large gene panel; and (iii) classification of gene variants following ACMG guidelines. Paediatric patients were defined as ≤16 years of age. The primary endpoint was ventricular arrhythmias (VAs). A total of 500 BrS patients were included, with 63 paediatric patients and 437 adult patients. Among children with BrS, 29 patients (46%) had a P/LP variant (P+) in SCN5A and no variants were found in 34 (54%) patients (P−). After a mean follow-up of 125.9 months, 8 children (12.7%) experienced a VA, treated with implanted cardioverter defibrillator shock. At survival analysis, P− paediatric patients had higher VA-free survival during the follow-up, compared with P+ paediatric patients. P+ status was an independent predictor of VA. There was no difference in VA-free survival between paediatric and adult BrS patients for both P− and P+.

Conclusion

In a large BrS cohort, the diagnostic yield for P/LP variants in the paediatric population is 46%. P+ children with BrS have a worse arrhythmic prognosis.
Incorporation, fate, and turnover of free fatty acids in cyanobacteria
Authors: Kahn Amaranta , Oliveira Paulo , Cuau Marine , Leão Pedro N
Published: April 2023

Fatty acids are important molecules in bioenergetics and also in industry. The phylum cyanobacteria consists of a group of prokaryotes that typically carry out oxygenic photosynthesis with water as an electron donor and use carbon dioxide as a carbon source to generate a range of biomolecules, including fatty acids. They are also able to import exogenous free fatty acids and direct them to biosynthetic pathways. Here, we review current knowledge on mechanisms and regulation of free fatty acid transport into cyanobacterial cells, their subsequent activation and use in the synthesis of fatty acid-containing biomolecules such as glycolipids and alka(e)nes, as well as recycling of free fatty acids derived from such molecules. This review also covers efforts in the engineering of such cyanobacterial fatty acid-associated pathways en route to optimized biofuel production.
Allometric Models and Biomass Conversion and Expansion Factors to Predict Total Tree-level Aboveground Biomass for Three Conifers Species in Iran
Authors: Ali Hassan , Mohammadi Jahangir , Shataee Jouibary Shaban
Published: April 2023

Accurate estimation of total aboveground biomass (TAGB) is an important challenge in evaluating and monitoring tree biomass. Thus, developing species-specific allometric models is essential. This study aimed to predict tree-level TAGB for Pinus brutia, Pinus pinea, Cupressus sempervirens, and the species-independent case using the most accurate allometric models, biomass conversion and expansion factor (BCEF), and mixed effect models in Golestan Province, Iran. The mean BCEFs for three species were 0.46, 0.47, and 0.86, respectively, and there was no significant difference (p>0.05) between TAGB predictions based on BCEF estimates for this study and observations of TAGB. The results revealed that compared with relative root mean square error (RMSE%) for the Intergovernmental Panel on Climate Change (IPCC) report–based BCEFs, the RMSE% for BCEFs estimated for this study were reduced by 46.91%. The results showed that a diameter at breast height (DBH), height (H), and wood density (ρ)-based model were the most accurate predictors for P. brutia (R2=0.98, RMSE%=14.11), whereas the DBH-based model and the DBH and H-based model were most accurate for P. pinea (R2=0.99, RMSE%=9.04) and C. sempervirens (R2=0.96, RMSE%=17.77), respectively. Compared to the allometric models, mixed-effect models using DBH, H, and ρ improved TAGB prediction for the species-independent case (3% increase in R2 and 6.81% decrease in RMSE%), but not for models for P. brutia, P. pinea, and C. sempervirens.

Study Implications: Accurate prediction of total tree-level aboveground biomass (TAGB) requires the most accurate allometric models plus accurate biomass conversion and expansion factors (BCEFs). The relevance of this study is that few allometric models have been developed to predict tree-level TAGB for Pinus brutia, Pinus pinea, and Cupressus sempervirens. We developed allometric models and estimated BCEFs for predicting TAGB for P. brutia, P. pinea, and C. sempervirens in Golestan Province, Iran. We provide accurate allometric models and BCEFs based on available field data. Also, we provide tools to help forest managers predict TAGB.
Predicting Impacts of Climate Change on Teak and Sal Forests in Central India Using Maximum Entropy Modeling: an Approach for Future Conservation and Silvicultural Strategies
Authors: Patasaraiya Maneesh Kumar , Devi Rinku Moni , Sinha Bhaskar , Bisaria Jigyasa
Published: April 2023

Climate change impacts on tropical forests and tree species have been documented as changes in distribution, growing period, phenology, habitat, productivity, species composition, and migration. This study attempts to assess the current and future habitat suitability distribution of two dominant species of Central India, teak (Tectona grandis Linn. f.) and sal (Shorea robusta Gaertn. f.) using a maximum entropy (MaxEnt) model to predict species occurrences by finding the distribution that has the most spread. The future suitable habitat ranges of the species were modeled for two time periods (2050 and 2070) and two representative concentration pathways (RCP 2.6 and 8.5). Representative concentration pathways are scenarios that describe alternative trajectories for carbon dioxide emissions and the resulting atmospheric concentration from 2000 to 2100. We collected and modeled the spatially well-dispersed species occurrence points of teak and sal. The results suggested an increase in suitable habitat range for teak and a decrease for sal for both time periods and RCPs. Furthermore, the jackknife analysis identified temperature and precipitation seasonality as the major variables that influence the distribution of teak. In the case of sal, minimum temperature makes the maximum contribution to distribution changes. The suitable silvicultural strategies for forest management are proposed based on the future distribution of species in relation to the climate variables responsible for the change in their distribution range. These findings and strategies will help forest departments build future management plans for teak and sal forest with a focus on minimizing the impact of climate change.

Study Implications: Our study used maximum entropy (MaxEnt) modeling to understand the impact of changing climate on the distribution of teak and sal forests of central India and to propose future silvicultural strategies. The study used MaxEnt model for two time periods and two climate change scenarios at highest resolution. An increase in future suitable habitat for teak and a decrease for sal is predicted by the model. Temperature for teak and precipitation for sal were identified as the major influencing climatic variables. We recommend forest and other related government departments to commission focused research to understand the changing patterns of species with climate change and design appropriate silviculture strategies for effective management.
Spatially Explicit Assessment of the USDA Forest Service as a Representative Bureaucracy
Authors: Locke Dexter H , Sachdeva Sonya S , Westphal Lynne M , Kenefic Laura S , Dockry Michael J , Fisher Cherie LeBlanc
Published: April 2023

Diverse workforce representation helps organizations achieve their goals and is important for government agencies that seek to gain public trust. Prior research has examined patterns of representation in the USDA Forest Service (Forest Service) and found an overall lack of representation despite advances at leadership levels. Federal agencies are required to report total workforce demographics; however, representation relative to local communities is rarely known. Using data from the US Census Bureau, we examined demographic profiles of populations within a 1-hour drive time around Forest Service workplaces relative to workforce demographics at each workplace. The Forest Service had a greater proportion of white employees than the US population as a whole, and racial/ethnicity diversity was lower than surrounding communities at 99.7% of Forest Service workplaces. Region-level summaries reinforce this pattern, suggesting the Forest Service is not a representative bureaucracy in any region or at any geographic scale. Given the Forest Service’s size, role in natural resources management, and geographic distribution of its workforce, disparities between demographic composition of the agency’s workforce and surrounding communities are striking. These results set a standard for spatially explicit assessments of workforce diversity as it relates to representative bureaucracy in geographically dispersed organizations like the Forest Service.

Study Implications: Prior research showed that the USDA Forest Service had proportionally fewer Black/African American (~3 times fewer) and Asian (~2 times fewer) employees than the US civilian labor force as a whole. However, aggregate agency-level statistics and national comparisons may conceal local and regional level variation. To understand how the racial and ethnic diversity at each workplace compared with its surrounding geographic area’s local population, 690 workplaces were examined. Results were consistent across multiple drive times and levels of aggregation; the Forest Service’s workforce is less diverse than surrounding communities, which may potentially affect its ability to serve its many stakeholders.
Determinants of Softwood Lumber Prices in the US Northwest
Authors: Reimer Jeffrey J , Annan Kenneth
Published: April 2023

Lumber prices can be volatile and hard to predict from month to month yet are important for many sectors of the economy, ranging from forestry and construction. An economic model of lumber prices was developed and applied to data representing multiple supply and demand determinants of lumber. Using a suite of econometric models, monthly lumber prices were related back to variables including construction permits, US reserve bank credit, tariffs with Canada, exchange rates with Canada, and variables representing shocks associated with the COVID-19 pandemic. Preferred models use relatively small amounts of publicly available information, making them more accessible to industry participants who want to make their own price predictions. Such information can help guide decisions about whether to expand or scale back an operation in preparation for likely future price movements.
Comparative analysis of fruit firmness and genes associated with cell wall metabolisms in three cultivated strawberries during ripening and postharvest
Authors: Ren Yibo , Li Baijun , Jia Haoran , Yang Xiaofang , Sun Yunfan , Shou Jiahan , Jiang Guihua , Shi Yanna , Chen Kunsong
Published: April 2023

Cultivated strawberry (Fragaria × ananassa), a world-famous fruit, is subjected to rapid softening during ripening, resulting in a shorter shelf life and severe economic losses during storage and transportation. However, there is limited understanding of the molecular mechanism underlying differences in fruit firmness during ripening and postharvest among cultivated strawberries. Here, we explored this molecular mechanism by comparing three cultivated strawberries via firmness measurement, transcriptome analysis, quantitative real-time polymerase chain reaction, and correlation analysis, and revealed FaEXP7, FaPG2, FaPLA, and Faβ-Gal4 as potential softening activators expressed before harvest to determine fruit with more softened texture and shorter shelf life, and that extremely high expression levels of FaCEL1-1 and FaCEL1-3 during ripening might be accelerators to intensify this situation. Additionally, both the enzyme activities of FaCEL and the expression pattern of FaCEL1-3 showed a significantly negative correlation with fruit firmness after harvest, suggesting that FaCEL1-3 might play a key role in promoting strawberry fruit softening not only during ripening but also postharvest. These results showed that the difference in fruit firmness and shelf life among cultivated strawberries was controlled by the temporal expression pattern of a legion of cell wall-associated genes during ripening and postharvest.
Evolution Under Dietary Restriction Increases Reproduction at the Cost of Decreased Somatic Growth
Authors: Huang Zhi-Yu , Xi Yi-Long , Wang Qiao , Li Zi-Ai , Shi Bao-Chun , Ge Ya-Li
Published: April 2023

Dietary restriction (DR) is cited as the most reliable means of extending life span in a wide range of taxa, yet the evolutionary basis of the DR effect on life span remains unclear. The resource reallocation hypothesis proposes that the longevity-extending response of DR is adaptive and stems from the reallocation of resources from reproduction to somatic maintenance under food-limited conditions. However, if DR continues for multiple generations, such a response becomes maladaptive, and genotypes with higher fecundity should be selectively favored over genotypes with longer longevity. To test this prediction, we exposed replicate populations of the rotifer Brachionus dorcas, a model organism for aging and experimental evolution studies, to DR and ad-libitum (AL) diets for 100 days. During the selection experiment, AL-selected populations showed higher growth rates and mictic ratios than DR-selected populations. After approximately 27 asexual generations of selection, populations with a DR selection history had a higher net reproductive rate but lower body volume and ingestion rate in the absence of survival costs than populations with an AL selection history when they were assayed on an AL diet. Our results are inconsistent with the prediction that evolution on sustained DR increases reproduction and reduces life span, and show for the first time that sustained DR selects for clones with higher energy investment in reproduction but lower investment in somatic growth.
Internalization or Dissociation? Negative Age Stereotypes Make You Feel Younger Now But Make You Feel Older Later
Authors: Kornadt Anna E , Weiss David , de Paula Couto Maria Clara , Rothermund Klaus
Published: April 2023

Objectives

Negative age stereotypes have negative, assimilative effects on the subjective aging experience due to internalization processes, but sometimes positive contrast effects are reported as well, reflecting dissociation and downward comparisons. Our aim was thus to compare short-term and long-term consequences of age stereotypes on the subjective aging experience, to test the hypothesis that contrast effects are visible cross-sectionally, whereas internalization processes are observed when considering long-term changes.

Methods

We assessed age stereotypes and subjective age in a core sample of N = 459 participants (initial age range 30–80 years) from the Ageing as Future project across 3 consecutive measurement occasions spanning a longitudinal interval of 10 years. Short-term and long-term effects were estimated with latent growth models by assessing the effects of age stereotypes on the intercepts (cross-sectional) and on the slopes (longitudinal) of subjective age, respectively, while controlling for current self-views.

Results

Age stereotypes had opposite effects on subjective age depending on the time frame. A cross-sectional contrast effect was found, whereas longitudinal effects were assimilative in nature.

Discussion

Our findings support the time-dependent nature of the effects of age stereotypes on the subjective aging experience. Negative age stereotypes temporarily lead to a significantly younger subjective age, indicating dissociation from one’s age group and downward comparison. In the long run, however, negative (positive) age stereotypes become internalized into the self-views of older people and are linked to a relatively older (younger) subjective age.
Community-Engaged Recommendations for Empowering People Living With Dementia to be Research Collaborators
Authors: Griffith Eric E , Monin Joan K , Lepore Michael
Published: April 2023

As cases of Alzheimer’s disease and related dementias (ADRD) increase worldwide, research design has placed additional emphasis on social and behavioral factors that affect ADRD symptomatology and quality of life. Despite this, few studies have incorporated people living with ADRD as research partners. We propose 5 community-engaged recommendations for incorporating people living with ADRD into future research as full collaborators. The proposed recommendations center the experiences of people living with ADRD as crucial contributions to scientific inquiry. The guidelines are based on experiences at a 2-day “Empowering Partnerships” workshop in 2019; post workshop activity continued through 2021 with ongoing collaborations, analysis, and reflective practice. The workshop and subsequent conversations engaged a network of people living with ADRD, informal carepartners, and researchers to collectively build their capacities to partner in all aspects of person-centered research. To empower people living with ADRD as research partners, we recommend that research teams (a) create a flexible schedule of communication and/or meetings to accommodate a wide range of ADRD symptoms, (b) generate team-specific communication strategies/guidelines, (c) incorporate lived experiences of people living with ADRD into research protocols, (d) involve people living with ADRD in all aspects of a project, beginning in the developmental stages, and (e) incorporate skilled facilitators to facilitate communication between stakeholder groups. This multi-vocal approach to research will diversify ADRD research and ensure that projects align with the priorities and capacities of principal stakeholders by incorporating individuals with a wide range of cognitive capabilities that more fully represent the diversity of ADRD experiences.
Estimating the occurrence of slow slip events and earthquakes with an ensemble Kalman filter
Authors: Diab-Montero Hamed Ali , Li Meng , van Dinther Ylona , Vossepoel Femke C
Published: April 2023

Our ability to forecast earthquakes and slow slip events is hampered by limited information on the current state of stress on faults. Ensemble data assimilation methods permit estimating the state by combining physics-based models and observations, while considering their uncertainties. We use an ensemble Kalman filter (EnKF) to estimate shear stresses, slip rates and the state θ acting on a fault point governed by rate-and-state friction embedded in a 1-D elastic medium. We test the effectiveness of data assimilation by conducting perfect model experiments. We assimilate noised shear-stress and velocity synthetic values acquired at a small distance to the fault. The assimilation of uncertain shear stress observations improves in particular the estimates of shear stress on fault segments hosting slow slip events, while assimilating observations of velocity improves their slip-rate estimation. Both types of observations help equally well to better estimate the state θ. For earthquakes, the shear stress observations improve the estimation of shear stress, slip rates and the state θ, whereas the velocity observations improve in particular the slip-rate estimation. Data assimilation significantly improves the estimates of the temporal occurrence of slow slip events and to a large extent also of earthquakes. Rapid and abrupt changes in velocity and shear stress during earthquakes lead to non-Gaussian priors for subsequent assimilation steps, which breaks the assumption of Gaussian priors of the EnKF. In spite of this, the EnKF still provides estimates that are unexpectedly close to the true evolution. In fact, the forecastability for earthquakes for the same alarm duration is very similar to slow slip events, having a very low miss rate with an alarm duration of just 10 per cent of the recurrence interval of the events. These results confirm that data assimilation is a promising approach for the combination of uncertain physics and indirect, noisy observations for the forecasting of both slow slip events and earthquakes.
Machine learning prediction of groundwater heights from passive seismic wavefield
Authors: Abi Nader A , Albaric J , Steinmann M , Hibert C , Malet J-P , Sue C , Fores B , Marchand A , Gros M , Celle H , Pohl B , Stefani V , Boetsch A
Published: April 2023

Most of water reservoirs are underground and therefore challenging to monitor. This is particularly the case of karst aquifers which knowledge is mostly based on sparse spatial and temporal observations. In this study, we propose a new approach, based on a supervised machine learning algorithm, the Random Forests, and continuous seismic noise records, that allows the prediction of the underground river water height. The study site is a karst aquifer in the Jura Mountains (France). An underground river is accessible through an artificial shaft and is instrumented by a hydrological probe. The seismic noise generated by the river is recorded by two broadband seismometers, located underground (20 m depth) and at the surface. The algorithm succeeds in predicting water height thanks to signal energy features. Even weak river-induced noise such as recorded at the surface can be detected and used by the algorithm. Its efficiency, expressed by the Nash–Sutcliffe criterion, is above 95 per cent and 53 per cent for data from the underground and surface seismic stations, respectively.
Laboratory investigation of hydraulic fracturing in granitic rocks using active and passive seismic monitoring
Authors: Butt A , Hedayat A , Moradian O
Published: April 2023

Knowledge of the fracturing processes can be important for the optimization of pressurized fluid injection operations in the deep underground rock mass. Active and passive seismic monitoring techniques have been used in the field for tracking or mapping the propagating hydraulic fracture. Although both these monitoring techniques provide valuable information about the generated fracture network, it is difficult for either technique to comprehensibly identify the different processes associated with hydraulic fracturing. The combined active and passive monitoring has the potential for better characterization of the complex hydraulic fracturing phenomena. In this study, laboratory hydraulic fracturing experiments with combined active and passive seismic monitoring were conducted on true triaxially loaded Barre granite cubes with different fluid injection rates. The seismic inelastic fracturing was detected by 16 passive acoustic emission sensors, where 3678 and 2370 seismic source events were detected for the high and low injection rate experiments, respectively. For active monitoring, strong variations in the attributes of signals were observed which were transmitted through four source–receiver pairs, placed both perpendicular and parallel to the generated hydraulic fracture. Positive velocity changes were observed for active sensor pairs with ray paths passing through the generated hydraulic fracture indicating fluid permeation, whereas isolated dry deformation was characterized by a slight but permanent velocity decrease. Compared to velocity, the energy of the active signals was 1–2 orders of magnitude more sensitive to different hydraulic fracturing processes. However, the sensitivity and signatures of the active signal attributes were found to be dependent on the frequency range and direction of ray path with respect to the location of the generated fracture network. Using the coupled evaluation of the active and passive signals we were able to systematically identify various hydraulic fracturing processes including: (1) aseismic deformation, (2) fracture initiation and fluid permeation, (3) pressure build-up, (4) fracture propagation and (5) pressure release and leak-off. The results of this study showed that combining the respective advantages of active and passive seismic techniques and using both of them to monitor the failure processes can facilitate a more comprehensive understanding and better control of the hydraulic stimulations in subsurface operations.
An appraisal using magnetic data of the continent-to-ocean transition structure west of Iberia
Authors: Neres Marta , Ranero César R
Published: April 2023

About half of the rifted margins purportedly formed by extension with minor magmatism. The conceptual models of those magma-poor systems are greatly influenced by the continent-to-ocean transition structure of the archetypal magma-poor West Iberia Margin. In the past, interpretation of magnetic data of West Iberia has been used to constrain conceptual rifting models, including the structure of the transition from the exhumed mantle domain to the oceanic crust formed at a spreading centre. However, uncertainties on geophysical data were generally not considered leading to overdetailed interpretations. We use synthetic magnetic modelling to show that magnetic data acquired at sea level cannot resolve subhorizontal lithological layering in deep-water continental margins. We then present new magnetic modelling guided by a refined velocity model of the wide-angle seismic IAM-9 profile that shows that the magnetic J-anomaly correlates with oceanic crust that abuts exhumed mantle across a vertical boundary. This well-constrained observation supports that seafloor spreading initiated abruptly, terminating mantle exhumation. Conventional wisdom dictates that the sudden efficient melt extraction relates to a mechanical threshold during lithospheric thinning and concomitant asthenospheric upwelling under which melt can migrate toward the surface. However, our results support that mantle melting creating oceanic crust was probably not driven by gradual lithospheric thinning and asthenospheric upwelling, but by seafloor spreading centre propagation that cut across the lithosphere, creating the abrupt structure.
Preoperative Use of Multiple Advanced Therapies Is Not Associated With Endoscopic Inflammatory Pouch Diseases
Authors: Powers Joseph Carter , Cohen Benjamin L , Rieder Florian , Click Benjamin H , Lyu Ruishen , Westbrook Katherine , Hull Tracy , Holubar Stefan , Regueiro Miguel D , Qazi Taha
Published: April 2023

Background

Patients with an ileal pouch-anal anastomosis (IPAA) can experience pouch inflammation postoperatively. The use of antitumor necrosis factor (anti-TNF) biologics may be associated with pouch inflammation, but limited data exist on the impact of multiple advanced therapies on development of subsequent pouch inflammation. The aim of this study was to assess for an association between preoperative use of multiple advanced therapies and risk of endoscopically detected inflammatory pouch diseases (EIPDs).

Methods

We performed a retrospective analysis of ulcerative colitis (UC) and indeterminate colitis (IBDU) patients who underwent an IPAA at a quaternary care center from January 2015 to December 2019. Patients were grouped based on number and type of preoperative drug exposures. The primary outcome was EIPD within 5 years of IPAA.

Results

Two hundred ninety-eight patients were included in this analysis. Most of these patients had UC (95.0%) and demonstrated pancolonic disease distribution (86.1%). The majority of patients were male (57.4%) and underwent surgery for medically refractory disease (79.2%). The overall median age at surgery was 38.6 years. Preoperatively, 68 patients were biologic/small molecule-naïve, 125 received anti-TNF agents only, and 105 received non-anti-TNF agents only or multiple classes. Ninety-one patients developed EIPD. There was no significant association between type (P = .38) or number (P = .58) of exposures and EIPD, but older individuals had a lower risk of EIPD (P = .001; hazard ratio, 0.972; 95% confidence interval, 0.956-0.989).

Conclusion

Development of EIPD was not associated with number or type of preoperative advanced therapies.
Beyond Dimorphism: Body Size Variation Among Adult Orangutans Is Not Dichotomous by Sex
Authors: Kralick Alexandra E , O'Connell Caitlin A , Bastian Meredith L , Hoke Morgan K , Zemel Babette S , Schurr Theodore G , Tocheri Matthew W
Published: April 2023

Synopsis

Among extant great apes, orangutans are considered the most sexually dimorphic in body size. However, the expression of sexual dimorphism in orangutans is more complex than simply males being larger than females. At sexual maturity, some male orangutans develop cheek pads (flanges), while other males remain unflanged even after becoming reproductively capable. Sometimes flange development is delayed in otherwise sexually mature males for a few years. In other cases, flange development is delayed for many years or decades, with some males even spending their entire lifespan as unflanged adults. Thus, unflanged males of various chronological ages can be mistakenly identified as “subadults.” Unflanged adult males are typically described as “female-sized,” but this may simply reflect the fact that unflanged male body size has only ever been measured in peri-pubescent individuals. In this study, we measured the skeletons of 111 wild adult orangutans (Pongo spp.), including 20 unflanged males, 45 flanged males, and 46 females, resulting in the largest skeletal sample of unflanged males yet studied. We assessed long bone lengths (as a proxy for stature) for all 111 individuals and recorded weights-at-death, femoral head diameters, bi-iliac breadths, and long bone cross-sectional areas (CSA) (as proxies for mass) for 27 of these individuals, including seven flanged males, three adult confirmed-unflanged males, and three young adult likely-unflanged males. ANOVA and Kruskal–Wallis tests with Tukey and Dunn post-hoc pairwise comparisons, respectively, showed that body sizes for young adult unflanged males are similar to those of the adult females in the sample (all P ≥ 0.09 except bi-iliac breadth), whereas body sizes for adult unflanged males ranged between those of adult flanged males and adult females for several measurements (all P < 0.001). Thus, sexually mature male orangutans exhibit body sizes that range from the female end of the spectrum to the flanged male end of the spectrum. These results exemplify that the term “sexual dimorphism” fails to capture the full range of variation in adult orangutan body size. By including adult unflanged males in analyses of body size and other aspects of morphology, not as aberrations but as an expected part of orangutan variation, we may begin to shift the way that we think about features typically considered dichotomous according to biological sex.