bluuebunny/crossref_metadata_2025 huggingface dataset:
Overexpression of pathogenic tau in astrocytes causes a reduction in AQP4 and GLT1, an immunosuppressed phenotype and unique transcriptional responses to repetitive mild TBI without appreciable changes in tauopathy
Authors: Ortiz Camila , Pearson Andrew , McCartan Robyn , Roche Shawn , Carothers Nolan , Browning Mackenzie , Perez Sylvia , He Bin , Ginsberg Stephen D. , Mullan Michael , Mufson Elliott J. , Crawford Fiona , Ojo Joseph
Published: May 2024

Epidemiological studies have unveiled a robust link between exposure to repetitive mild traumatic brain injury (r-mTBI) and elevated susceptibility to develop neurodegenerative disorders, notably chronic traumatic encephalopathy (CTE). The pathogenic lesion in CTE cases is characterized by the accumulation of hyperphosphorylated tau in neurons around small cerebral blood vessels which can be accompanied by astrocytes that contain phosphorylated tau, the latter termed tau astrogliopathy. However, the contribution of tau astrogliopathy to the pathobiology and functional consequences of r-mTBI/CTE or whether it is merely a consequence of aging remains unclear. We addressed these pivotal questions by utilizing a mouse model harboring tau-bearing astrocytes, GFAPP301L mice, subjected to our r-mTBI paradigm. Despite the fact that r-mTBI did not exacerbate tau astrogliopathy or general tauopathy, it increased phosphorylated tau in the area underneath the impact site. Additionally, gene ontology analysis of tau-bearing astrocytes following r-mTBI revealed profound alterations in key biological processes including immunological and mitochondrial bioenergetics. Moreover, gene array analysis of microdissected astrocytes accrued from stage IV CTE human brains revealed an immunosuppressed astroglial phenotype similar to tau-bearing astrocytes in the GFAPP301L model. Additionally, hippocampal reduction of proteins involved in water transport (AQP4) and glutamate homeostasis (GLT1) was found in the mouse model of tau astrogliopathy. Collectively, these findings reveal the importance of understanding tau astrogliopathy and its role in astroglial pathobiology under normal circumstances and following r-mTBI. The identified mechanisms using this GFAPP301L model may suggest targets for therapeutic interventions in r-mTBI pathogenesis in the context of CTE.
The recovery processes among paramedics who encountered violence during work—a narrative interview study
Authors: Kamaja Veera , Nordquist Hilla
Published: May 2024

Background

Almost all paramedics encounter workplace violence (WPV) during their careers. The most common form of WPV is verbal, and the perpetrator is usually the patient. It is known that paramedics suffer from post-traumatic stress disorder and other mental health problems, and WPV is one of the reasons behind that. Nevertheless, little is known about the recovery processes paramedics have had after encountering WPV. The research question was: What kind of recovery processes have paramedics had after encountering WPV?

Methods

A qualitative, narrative interview study was done. Data was collected in individual interviews with Finnish paramedics (n = 18). Paramedics were from different parts of Finland, and their ages varied from 24 to 49 years. They had been working in EMS for an average of 10.5 years (range 1.5 to 25 years). Interviews were conducted with a narrative approach, which enabled paramedics to narrate their experiences and speak on their own terms about the subject to the extent of their choosing. The data was analyzed using thematic analysis.

Results

Ten recovery process themes were identified: Strong psychological and physical reactions in a short time frame, Questioning one’s profession and actions, Various support structures aided in recovery, Dysfunctional processes hindered recovery, Personal resources provided support, The support of the workcommunity as a lifeline, Left to cope alone, Permanent changes to work routines, Resulting in professional growth and Eternal crack in the shell.

Conclusions

Many internal and external factors affect paramedics’ recovery processes. While some receive adequate help, others struggle to get appropriate support, especially from their organization and supervisors. The findings of this study suggest that clear protocols should be established to help paramedics recover after encountering WPV and that an individual aspect should be kept in mind, as not everybody reacts in the same way.
Assessment of subjective well-being of healthcare workers in response to heat and personal protective equipment under controlled conditions using a standardized protocol
Authors: Quartucci Caroline , Wibowo Razan , Do Viet , Bose-O`Reilly Stephan , Nowak Dennis , Weilnhammer Veronika , Weinmann Tobias , Rakete Stefan
Published: May 2024

Background

Due to climate change, the increasing frequency of hot summer days and heat waves can result in occupational heat strain, especially in non-air-conditioned workplaces. Healthcare workers (HCW) engaged in patient care are particularly affected, as they are additionally exposed to physical stress. The use of personal protective equipment (PPE) can aggravate heat strain in HCW. This study aimed to examine the subjective well-being of HCW when exposed to heat and PPE under controlled conditions.

Methods

This study was designed as a randomized crossover trial. Participants performed standardized healthcare tasks in a climatic chamber for approximately 3.5 h at different indoor temperatures (22 °C and 27 °C) and varied working conditions (with or without PPE). The effects on participants’ subjective well-being, encompassing thermal, physiological and psychological stress were assessed using a customized questionnaire.

Results

Heat had a greater effect than PPE on thermal, physical and psychological stress. Conversely, PPE had a greater effect on physical demand and effort. For the majority of outcomes, combined exposure to heat and PPE resulted in the highest perceived discomfort. Furthermore, the participants reported increased sweating and other discomforts when working at elevated temperatures or with PPE.

Conclusions

In this study, heat and PPE, but particularly the combination of both factors, were identified as unfavorable working environments. Although the trials were conducted in a controlled environment, the outcomes provide valuable information about the effect of heat and PPE on HCW in a real-life setting. Furthermore, the design used in this study can be beneficial in evaluating the effect of mitigation strategies.
Halitosis in young patients with chronic kidney disease: findings from a randomized controlled trial
Authors: Hoefer Karolin Charlotte , Barbe Anna Greta , Adams Anne , Schoppmeier Christoph , Wicht Michael Jochen , Weber Lutz T , Noack Michael J , Graf Isabelle
Published: May 2024

Background

Chronic kidney disease (CKD) directly affects oral health. Yet data about halitosis in young CKD patients and the impact of dental prophylaxis is limited. Therefore, as part of this randomized clinical trial, halitosis in young CKD patients undergoing intensive or standard oral preventive procedures was to be explored.

Methods

Three volatile sulfur compounds (hydrogen sulfide, methyl mercaptan and dimethyl sulfide) were measured in 30 young patients with CKD (mean age 14.2 years; 16 males, 14 females). Breath samples were taken after 3 and 6 months and analyzed with selective gas chromatography (OralChroma). Tongue coating (Winkel Index) and clinical indices to determine local inflammation or oral hygiene (Papillary Bleeding Index and Quigley-Hein Index) were assessed. Within an extended anamnesis, patients and their mothers and nurses were questioned about the perceived halitosis. Corresponding quotes were noted verbatim. Patients were randomized to either intensive need-related oral health care measures (oral preventative program, OPP) or a one-stage standard prevention (treatment as usual, TAU).

Results

While there were no differences in volatile sulfur compound levels between TAU and OPP at the three time points of measurements (p > 0.05), there was a tendency towards a reduction in dimethyl sulfide and hydrogen sulfide of affected patients within the OPP group over time. Looking at potential differences between both groups with regard to tongue coating, significant differences were observed between baseline and 3 months after study start in the OPP group, and between baseline and 6 months after study start in the TAU group (p < 0.05). The burden of halitosis was frequently reported by patients’ mothers and nurses.

Conclusions

Young CKD patients regularly suffered from halitosis and dimethyl sulfide was its main source. Preventive measures mainly resulted in a reduction of tongue coating. Trial registration: The German Clinical Trial Register (# DRKS00010580).
Moxibustion ameliorates chronic inflammatory visceral pain via spinal circRNA-miRNA-mRNA networks: a central mechanism study
Authors: Zhang Dan , Dong Xiaoqing , Li Xiaoying , Yang Yanting , Li Hongna , Hong Yue , Yang Guang , Kong Xiehe , Wang Xuejun , Ma Xiaopeng
Published: May 2024

This study aimed to unveil the central mechanism of moxibustion treating chronic inflammatory visceral pain (CIVP) from the angle of circRNA-miRNA-mRNA networks in the spinal cord. The rat CIVP model was established using a mixture of 5% (w/v) 2,4,6-trinitrobenzene sulfonic acid and 50% ethanol at a volume ratio of 2:1 via enema. Rats in the moxibustion group received herb-partitioned moxibustion at Tianshu (ST25, bilateral) and Qihai (CV6) points. The abdominal withdrawal reflex (AWR), mechanical withdrawal threshold (MWT), and thermal withdrawal latency (TWL) were adopted for pain behavior observation and pain sensitivity assessment. The circRNA, miRNA, and mRNA expression profiles were detected using the high-throughput sequencing technique. Relevant databases and bioinformatics analysis methods were used to screen for differentially expressed (DE) RNAs and build a circRNA-miRNA-mRNA (competing endogenous RNA) ceRNA regulatory network. The real-time quantitative PCR was employed to verify the sequencing result. CIVP rat models had a significantly higher AWR and lower TWL and MWT than normal rats. Between normal and model rats, there were 103 DE-circRNAs, 16 DE-miRNAs, and 397 DE-mRNAs in the spinal cord. Compared with the model group, the moxibustion group had a lower AWR and higher TWL and MWT; between these two groups, there were 118 DE-circRNAs, 15 DE-miRNAs, and 804 DE-mRNAs in the spinal cord. Two ceRNA networks were chosen to be verified. As a result, moxibustion’s analgesic effect on visceral pain in CIVP rats may be associated with regulating the circRNA_02767/rno-miR-483-3p/Gfap network in the spinal cord and improving central sensitization.
PAX6 promotes neuroendocrine phenotypes of prostate cancer via enhancing MET/STAT5A-mediated chromatin accessibility
Authors: Jing Nan , Du Xinxing , Liang Yu , Tao ZhenKeke , Bao Shijia , Xiao Huixiang , Dong Baijun , Gao Wei-Qiang , Fang Yu-Xiang
Published: May 2024

Background

Neuroendocrine prostate cancer (NEPC) is a lethal subset of prostate cancer which is characterized by neuroendocrine differentiation and loss of androgen receptor (AR) signaling. Growing evidence reveals that cell lineage plasticity is crucial in the failure of NEPC therapies. Although studies suggest the involvement of the neural transcription factor PAX6 in drug resistance, its specific role in NEPC remains unclear.

Methods

The expression of PAX6 in NEPC was identified via bioinformatics and immunohistochemistry. CCK8 assay, colony formation assay, tumorsphere formation assay and apoptosis assay were used to illustrate the key role of PAX6 in the progression of in vitro. ChIP and Dual-luciferase reporter assays were conducted to confirm the binding sequences of AR in the promoter region of PAX6, as well as the binding sequences of PAX6 in the promoter regions of STAT5A and MET. For in vivo validation, the xenograft model representing NEPC subtype underwent pathological analysis to verify the significant role of PAX6 in disease progression. Complementary diagnoses were established through public clinical datasets and transcriptome sequencing of specific cell lines. ATAC-seq was used to detect the chromatin accessibility of specific cell lines.

Results

PAX6 expression was significantly elevated in NEPC and negatively regulated by AR signaling. Activation of PAX6 in non-NEPC cells led to NE trans-differentiation, while knock-down of PAX6 in NEPC cells inhibited the development and progression of NEPC. Importantly, loss of AR resulted in an enhanced expression of PAX6, which reprogramed the lineage plasticity of prostate cancer cells to develop NE phenotypes through the MET/STAT5A signaling pathway. Through ATAC-seq, we found that a high expression level of PAX6 elicited enhanced chromatin accessibility, mainly through attenuation of H4K20me3, which typically causes chromatin silence in cancer cells.

Conclusion

This study reveals a novel neural transcription factor PAX6 could drive NEPC progression and suggest that it might serve as a potential therapeutic target for the management of NEPC.
Improved biological methanation using tubular foam-bed reactor
Authors: Khesali Aghtaei Hoda , Heyer Robert , Reichl Udo , Benndorf Dirk
Published: May 2024

Background

Power-to-gas is the pivotal link between electricity and gas infrastructure, enabling the broader integration of renewable energy. Yet, enhancements are necessary for its full potential. In the biomethanation process, transferring H2 into the liquid phase is a rate-limiting step. To address this, we developed a novel tubular foam-bed reactor (TFBR) and investigated its performance at laboratory scale.

Results

A non-ionic polymeric surfactant (Pluronic® F-68) at 1.5% w/v was added to the TFBR’s culture medium to generate a stabilized liquid foam structure. This increased both the gas–liquid surface area and the bubble retention time. Within the tubing, cells predominantly traveled evenly suspended in the liquid phase or were entrapped in the thin liquid film of bubbles flowing inside the tube. Phase (I) of the experiment focused primarily on mesophilic (40 °C) operation of the tubular reactor, followed by phase (II), when Pluronic® F-68 was added. In phase (II), the TFBR exhibited 6.5-fold increase in biomethane production rate (MPR) to 15.1 $$({\text{L}}_{{\text{CH}}_{4}}\text{/}{\text{L}}_{\text{R}}\text{/d)}$$

(

L

CH
4

/L
R

/d)

, with a CH4 concentration exceeding 90% (grid quality), suggesting improved H2 transfer. Transitioning to phase (III) with continuous operation at 55 °C, the MPR reached 29.7 $${\text{L}}_{{\text{CH}}_{4}}\text{/}{\text{L}}_{\text{R}}\text{/d}$$

L

CH
4

/L
R

/d

while maintaining the grid quality CH4. Despite, reduced gas–liquid solubility and gas–liquid mass transfer at higher temperatures, the twofold increase in MPR compared to phase (II) might be attributed to other factors, i.e., higher metabolic activity of the methanogenic archaea.

To assess process robustness for phase (II) conditions, a partial H2 feeding regime (12 h 100% and 12 h 10% of the nominal feeding rate) was implemented. Results demonstrated a resilient MPR of approximately 14.8 $${\text{L}}_{{\text{CH}}_{4}}\text{/}{\text{L}}_{\text{R}}\text{/d}$$

L

CH
4

/L
R

/d

even with intermittent, low H2 concentration.

Conclusions

Overall, the TFBR’s performance plant sets the course for an accelerated introduction of biomethanation technology for the storage of volatile renewable energy. Robust process performance, even under H2 starvation, underscores its reliability. Further steps towards an optimum operation regime and scale-up should be initiated. Additionally, the use of TFBR systems should be considered for biotechnological processes in which gas–liquid mass transfer is a limiting factor for achieving higher reaction rates.
MiR-320 inhibits PRRSV replication by targeting PRRSV ORF6 and porcine CEBPB
Authors: Gao Xiaoxiao , You Xiangbin , Wang Guowei , Liu Mengtian , Ye Longlong , Meng Yufeng , Luo Gan , Xu Dequan , Liu Min
Published: May 2024

Porcine reproductive and respiratory syndrome (PRRS), a highly contagious disease caused by Porcine reproductive and respiratory syndrome virus (PRRSV), results in huge economic losses to the world pig industry. MiRNAs have been reported to be involved in regulation of viral infection. In our study, miR-320 was one of 21 common differentially expressed miRNAs of Meishan, Pietrain, and Landrace pig breeds at 9-h post-infection (hpi). Bioinformatics and experiments found that PRRSV replication was inhibited by miR-320 through directly targeting PRRSV ORF6. In addition, the expression of CCAAT enhancer binding protein beta (CEBPB) was also inhibited by miR-320 by targeting the 3ʹ UTR of CEBPB, which significantly promotes PRRSV replication. Intramuscular injection of pEGFP-N1-miR-320 verified that miR-320 significantly inhibited the replication of PRRSV and alleviated the symptoms caused by PRRSV in piglets. Taken together, miR-320 have significant roles in the infection and may be promising therapeutic target for PRRS.
Classical BSE dismissed as the cause of CWD in Norwegian red deer despite strain similarities between both prion agents
Authors: Marín-Moreno Alba , Benestad Sylvie L. , Barrio Tomas , Pirisinu Laura , Espinosa Juan Carlos , Tran Linh , Huor Alvina , Di Bari Michele Angelo , Eraña Hasier , Maddison Ben C. , D’Agostino Claudia , Fernández-Borges Natalia , Canoyra Sara , Jerez-Garrido Nuria , Castilla Joaquín , Spiropoulos John , Bishop Keith , Gough Kevin C. , Nonno Romolo , Våge Jorn , Andréoletti Olivier , Torres Juan María
Published: May 2024

The first case of CWD in a Norwegian red deer was detected by a routine ELISA test and confirmed by western blotting and immunohistochemistry in the brain stem of the animal. Two different western blotting tests were conducted independently in two different laboratories, showing that the red deer glycoprofile was different from the Norwegian CWD reindeer and CWD moose and from North American CWD. The isolate showed nevertheless features similar to the classical BSE (BSE-C) strain. Furthermore, BSE-C could not be excluded based on the PrPSc immunohistochemistry staining in the brainstem and the absence of detectable PrPSc in the lymphoid tissues. Because of the known ability of BSE-C to cross species barriers as well as its zoonotic potential, the CWD red deer isolate was submitted to the EURL Strain Typing Expert Group (STEG) as a BSE-C suspect for further investigation. In addition, different strain typing in vivo and in vitro strategies aiming at identifying the BSE-C strain in the red deer isolate were performed independently in three research groups and BSE-C was not found in it. These results suggest that the Norwegian CWD red deer case was infected with a previously unknown CWD type and further investigation is needed to determine the characteristics of this potential new CWD strain.
Microbiota-derived 3-phenylpropionic acid promotes myotube hypertrophy by Foxo3/NAD+ signaling pathway
Authors: Li Penglin , Feng Xiaohua , Ma Zewei , Yuan Yexian , Jiang Hongfeng , Xu Guli , Zhu Yunlong , Yang Xue , Wang Yujun , Zhu Canjun , Wang Songbo , Gao Ping , Jiang Qingyan , Shu Gang
Published: May 2024

Background

Gut microbiota and their metabolites play a regulatory role in skeletal muscle growth and development, which be known as gut-muscle axis. 3-phenylpropionic acid (3-PPA), a metabolite produced by colonic microorganisms from phenylalanine in the gut, presents in large quantities in the blood circulation. But few study revealed its function in skeletal muscle development.

Results

Here, we demonstrated the beneficial effects of 3-PPA on muscle mass increase and myotubes hypertrophy both in vivo and vitro. Further, we discovered the 3-PPA effectively inhibited protein degradation and promoted protein acetylation in C2C12 and chick embryo primary skeletal muscle myotubes. Mechanistically, we supported that 3-PPA reduced NAD+ synthesis and subsequently suppressed tricarboxylic acid cycle and the mRNA expression of SIRT1/3, thus promoting the acetylation of total protein and Foxo3. Moreover, 3-PPA may inhibit Foxo3 activity by directly binding.

Conclusions

This study firstly revealed the effect of 3-PPA on skeletal muscle growth and development, and newly discovered the interaction between 3-PPA and Foxo3/NAD+ which mechanically promote myotubes hypertrophy. These results expand new understanding for the regulation of gut microbiota metabolites on skeletal muscle growth and development.
Deep video-based person re-identification (Deep Vid-ReID): comprehensive survey
Authors: Saad Rana S. M. , Moussa Mona M. , Abdel-Kader Nemat S. , Farouk Hesham , Mashaly Samia
Published: May 2024

Person re-identification (ReID) aims to find the person of interest across multiple non-overlapping cameras. It is considered an essential step for person tracking applications which is vital for surveillance. Person ReID could be investigated either using image-based or video-based. Video-based person ReID is considered more discriminating and realistic than image-based ReID due to the massive information extracted for each person. Different deep-learning techniques have been used for video-based ReID. In this survey, recently published articles are reviewed according to video-based ReID system pipeline: deep features learning, deep metric learning, and deep learning approaches. The deep feature learning approaches are categorized into spatial and temporal approaches, while deep metric learning is divided into metric and metric learning approaches. The deep learning approaches are differentiated into: supervised, unsupervised, weakly-supervised, and one-shot learning. A detailed analysis is held for the architectures of the state-of-the-art deep learning approaches. And their performance on four benchmark datasets is compared.
The adsorption effect of aquaculture residual feed on heavy metals and its impact on microbial metabolism of feed
Authors: Wang Sufeng , Wang Zijng , Zhang Wen , Li Jingshan , Lin Mingyang , Chen Li
Published: May 2024

Background

Intensive and large-scale aquaculture has a high biological density, and a large amount of artificial feed must be provided. As a result, residual feed would be discharged into natural water bodies with water exchange. Due to heavy metal pollution in surface water, residual feed may adsorb heavy metals in the water, affecting the subsequent microbial degradation of feed and the restoration of the water environment. The combined ecological impacts of these two types of pollution urgently need to be explored.

Results

This study explored the adsorption effect of residual feed on heavy metals and its impact on microbial degradation of residual feed. The results showed that both the insoluble component (mainly fiber, ash, and ether extract, abbreviated as FAE) and soluble component (soluble protein and starch) of the feed had adsorption effects on heavy metals. As the salinity increased, the adsorption capacity of Cu2+ and Cd2+ decreased, while the adsorption effect on Pb2+ was not changed significantly. The adsorption of heavy metals by residual feed could affect the biological degradation of residual feed. The residual feed could adsorb heavy metals and bacterial cells simultaneously, forming FAE-B(bacteria)-S(starch)-Pr(protein)-metal adsorption complex. This adsorption effect could reduce the negative impact of heavy metals on microbial degradation of residual feed. The simulated degradation experiments in actual water bodies also confirmed this. However, the accumulation of heavy metals in residual feed may bring negative effects, such as being consumed by aquatic organisms and entering the food chain.

Conclusions

The results of this study provide a basis for the collection and centralized treatment of residual feed in aquaculture, as well as the control and remediation of residual feed pollution in natural water bodies.

Graphical Abstract
Lithium-discontinuation-induced treatment refractoriness revisited
Authors: Kupka Ralph , Regeer Eline , van Bergen Annet , Tondo Leonardo , Bauer Michael
Published: May 2024

Background

Lithium is effective in the long-term treatment of bipolar disorder. Concerns have been raised about non-responsiveness after discontinuation and resuming previously effective lithium prophylaxis. We reviewed the available literature on this so-called lithium-discontinuation-induced treatment refractoriness (LDITR).

Results

We found 11 case reports and six cohort studies including 403 patients addressing LDITR, and one nation-wide register study providing some additional data on LDITR. Pooling all cohort studies, the percentages of non-responders during re-treatment with lithium ranged from 3.6 to 27.7%, with an average of 17.3%. Non-responsiveness was associated with longer duration of lithium treatment before discontinuation, longer duration of bipolar disorder before start of lithium, faster tapering off lithium, and longer duration of discontinuation.

Conclusions

There may be a subgroup in whom lithium discontinuation-induced treatment refractoriness exists. However, the vast majority of people respond when lithium is restarted. Moreover, it may be necessary to continue lithium beyond the first relapses to restore long-term prophylactic efficacy.
Risk factors of substance use treatment gaps among a nationally representative sample of black American adults in relation to sexual minority status and health insurance coverage
Authors: Rice Josiah K. , Anderson-Carpenter Kaston D. , Ellis Javon D.
Published: May 2024

Background

Little research has investigated predictors of specialty substance use treatment gaps among Black adults. This study examined differential odds of experiencing self-reported, past-year treatment gaps among Black adults with respect to sexual minority status and health insurance coverage, accounting for social cofactors.

Method

This cross-sectional study comprised 36,098 Black Americans aged 18 and older who completed the 2015–2019 National Survey on Drug Use and Health (NSDUH) and provided responses for all selected survey items. Design-based multivariable logistic regression models were used to examine predictors of drug and alcohol treatment gaps.

Results

Sexual minority Black adults reported greater odds of experiencing treatment gaps to specialty treatment (i.e., inpatient hospital, inpatient/outpatient rehabilitation facility, or mental health center) compared to Black heterosexuals in adjusted models (Gay or lesbian: AOR = 2.01, 95% CI = 1.39–2.89; Bisexual: AOR = 2.35, 95% CI = 1.77–3.12), with bisexual Black women experiencing the greatest odds (AOR = 3.10, 95% CI = 2.33–4.14). Black adults with no health insurance were significantly more likely to report substance use treatment gaps relative to their peers with health insurance coverage (AOR = 50, 95% CI = 1.26–1.78).

Conclusion

The results suggest a critical need for more investigations into patterns of specialty substance use treatment gaps within Black populations and for developing sexual identity-affirming mechanisms for closing the disparity gap, particularly for Black sexual minorities and those who lack health insurance coverage.
Application of 3D laser scanning technology for mapping and accuracy assessment of the point cloud model for the Great Achievement Palace heritage building
Authors: Liu Sha , Bin Mamat Mohd Jaki
Published: May 2024

Heritage buildings represent history and act as vessels of human civilisation. The significance of these architectural phenomena increases as society increases, which renders their preservation and ethical use increasingly crucial. Hence, preserving heritage buildings is necessary for societal benefit. The accuracy of three-dimensional (3D) point cloud models of heritage buildings is important to their digital conservation. Traditional mapping methods typically require more time and human resources. Furthermore, the outcome is subject to measurement omissions, errors, and other issues, which are contrary to the protection of the object measured. The complex elements of ancient Chinese architecture render it challenging for traditional measuring techniques to accurately capture spatial structural information. The 3D laser scanning technology is a novel technology to obtain 3D data rapidly. In this paper, the Great Achievement Palace of the Confucian Temple in Yuci District, Jinzhong City, Shanxi Province, was used as an example to examine the application of 3D laser scanning technology to acquire point cloud models of heritage buildings. Field data collection for architectural heritage using 3D laser scanning technology requires measuring station setting analysis to ensure effect accuracy while considering the elements of target location and quantity. For the 3D point cloud model of the Great Achievement Palace, error analysis and accuracy assessment were conducted on the quality elements of alignment accuracy between scanning stations, point cloud model reliability, point cloud data noise condition, and whether the point cloud data were stratified. The findings demonstrated that the improved method quickly and accurately acquired 3D point cloud model data and obtained realistic spatial data models of the heritage building.
Mechanism of vertical displacement beneath shallow compression zone in coastal area
Authors: Tran Duc-Huy , Wang Shih-Jung , Chen Jian-Yu
Published: May 2024

Previous studies showed that there is a discrepancy between total subsidence (measured on the ground surface) and shallow compression (measured within several hundred meters beneath the ground surface) in Taiwan. This difference is referred to as deep displacement in this study. The variations of deep displacement are opposite to those of ground surface displacement and groundwater level within the depth of several hundred meters. The mechanism is unknown and requires further investigation. This study adopts two kinds of geodetic observation data and separates land subsidence into shallow compression and deep displacement to investigate the mechanism of deep displacement. A tectonically active coastal area in Taiwan is selected as the study area. The assessment results show that the associated variations are likely due to cyclic hydraulic loading and unloading. The variations of deep displacement are opposite to those of ground surface displacement. This study proposes that these variations are due to hydraulic expansion and contraction. The mechanism is demonstrated using a hydromechanical model. The results of a cross-correlation analysis show that hydraulic expansion and contraction occur at certain depths. The study results provide important information on the mechanism of deep displacement that can be used in tectonophysical and land subsidence investigations.

Graphical Abstract
A CT based radiomics analysis to predict the CN0 status of thyroid papillary carcinoma: a two- center study
Authors: Li Zongbao , Zhong Yifan , Lv Yan , Zheng Jianzhong , Hu Yu , Yang Yanyan , Li Yunxi , Sun Meng , Liu Siqian , Guo Yan , Zhang Mengchao , Zhou Le
Published: May 2024

Objectives

To develop and validate radiomics model based on computed tomography (CT) for preoperative prediction of CN0 status in patients with papillary thyroid carcinoma (PTC).

Methods

A total of 548 pathologically confirmed LNs (243 non-metastatic and 305 metastatic) two distinct hospitals were retrospectively assessed. A total of 396 radiomics features were extracted from arterial-phase CT images, where the strongest features containing the most predictive potential were further selected using the least absolute shrinkage and selection operator (LASSO) regression method. Delong test was used to compare the AUC values of training set, test sets and cN0 group.

Results

The Rad-score showed good discriminating performance with Area Under the ROC Curve (AUC) of 0.917(95% CI, 0.884 to 0.950), 0.892 (95% CI, 0.833 to 0.950) and 0.921 (95% CI, 868 to 0.973) in the training, internal validation cohort and external validation cohort, respectively. The test group of CN0 with a AUC of 0.892 (95% CI, 0.805 to 0.979). The accuracy was 85.4% (sensitivity = 81.3%; specificity = 88.9%) in the training cohort, 82.9% (sensitivity = 79.0%; specificity = 88.7%) in the internal validation cohort, 85.4% (sensitivity = 89.7%; specificity = 83.8%) in the external validation cohort, 86.7% (sensitivity = 83.8%; specificity = 91.3%) in the CN0 test group.The calibration curve demonstrated a significant Rad-score (P-value in H-L test > 0.05). The decision curve analysis indicated that the rad-score was clinically useful.

Conclusions

Radiomics has shown great diagnostic potential to preoperatively predict the status of cN0 in PTC.
Uncoupled: investigating the lack of correlation between the transcription of putative plastic-degrading genes in the global ocean microbiome and marine plastic pollution
Authors: Gambarini Victor , Drost Cornelis J. , Kingsbury Joanne M. , Weaver Louise , Pantos Olga , Handley Kim M. , Lear Gavin
Published: May 2024

Background

Plastic pollution is a severe threat to marine ecosystems. While some microbial enzymes can degrade certain plastics, the ability of the global ocean microbiome to break down diverse environmental plastics remains limited. We employed metatranscriptomic data from an international ocean survey to explore global and regional patterns in microbial plastic degradation potential.

Results

On a global oceanic scale, we found no significant correlation between levels of plastic pollution and the expression of genes encoding enzymes putatively identified as capable of plastic degradation. Even when looking at different regional scales, ocean depth layers, or plastic types, we found no strong or even moderate correlation between plastic pollution and relative abundances of transcripts for enzymes with presumed plastic biodegradation potential. Our data, however, indicate that microorganisms in the Southern Ocean show a higher potential for plastic degradation, making them more appealing candidates for bioprospecting novel plastic-degrading enzymes.

Conclusion

Our research contributes to understanding the complex global relationship between plastic pollution and microbial plastic degradation potential. We reveal that the transcription of putative plastic-degrading genes in the global ocean microbiome does not correlate to marine plastic pollution, highlighting the ongoing danger that plastic poses to marine environments threatened by plastic pollution.
Promoting equality, diversity and inclusion in research and funding: reflections from a digital manufacturing research network
Authors: Fisher Oliver J. , Fearnshaw Debra , Watson Nicholas J. , Green Peter , Charnley Fiona , McFarlane Duncan , Sharples Sarah
Published: May 2024

Background

Equal, diverse, and inclusive teams lead to higher productivity, creativity, and greater problem-solving ability resulting in more impactful research. However, there is a gap between equality, diversity, and inclusion (EDI) research and practices to create an inclusive research culture. Research networks are vital to the research ecosystem, creating valuable opportunities for researchers to develop their partnerships with both academics and industrialists, progress their careers, and enable new areas of scientific discovery. A feature of a network is the provision of funding to support feasibility studies – an opportunity to develop new concepts or ideas, as well as to ‘fail fast’ in a supportive environment. The work of networks can address inequalities through equitable allocation of funding and proactive consideration of inclusion in all of their activities.

Methods

This study proposes a strategy to embed EDI within research network activities and funding review processes. This paper evaluates 21 planned mitigations introduced to address known inequalities within research events and how funding is awarded. EDI data were collected from researchers engaging in a digital manufacturing network activities and funding calls to measure the impact of the proposed method.

Results

Quantitative analysis indicates that the network’s approach was successful in creating a more ethnically diverse network, engaging with early career researchers, and supporting researchers with care responsibilities. However, more work is required to create a gender balance across the network activities and ensure the representation of academics who declare a disability. Preliminary findings suggest the network’s anonymous funding review process has helped address inequalities in funding award rates for women and those with care responsibilities, more data are required to validate these observations and understand the impact of different interventions individually and in combination.

Conclusions

In summary, this study offers compelling evidence regarding the efficacy of a research network's approach in advancing EDI within research and funding. The network hopes that these findings will inform broader efforts to promote EDI in research and funding and that researchers, funders, and other stakeholders will be encouraged to adopt evidence-based strategies for advancing this important goal.
[68Ga]Ga-NODAGA-TriGalactan, a low molecular weight tracer for the non-invasive imaging of the functional liver reserve
Authors: Zierke Maximilian A. , Rangger Christine , Samadikhah Kimia , Panzer Marlene , Dichtl Stefanie , Hörmann Nikolas , Wilflingseder Doris , Schmid Andreas M. , Haubner Roland
Published: May 2024

Background

Determination of the functional liver mass is important in a variety of clinical settings including liver surgery and transplantation. [99mTc]Tc-diethylenetriamine-pentaacetic acid galactosyl human serum albumin (99mTc-GSA) is a radiotracer targeting the asialoglycoprotein receptor (ASGR) and is routinely used in Japan for this purpose. Here we describe the development and evaluation of [68Ga]Ga-NODAGA-TriGalactan a low molecular weight PET-tracer targeting this structure.

Results

For synthesis TRIS as branching unit and NODAGA as chelator for labelling with [68Ga]Ga are included. Three galactose moieties are conjugated via a click chemistry approach resulting in the desired labelling precursor.68Ga-labelling could be accomplished in high radiochemical yield and purity. [68Ga]Ga-NODAGA-TriGalactan is very hydrophilic and revealed high plasma stability and low plasma protein binding. Fluorescence imaging showed binding on ASGR-positive organoids and the IC50-value was in the nanomolar range. Most importantly, both biodistribution as well as animal imaging studies using normal mice demonstrated high liver uptake with rapid elimination from all other organs leading to even higher liver-to-background ratios as found for 99mTc-GSA.

Conclusion

[68Ga]Ga-NODAGA-TriGalactan shows high in vitro stability and selectively binds to the ASGR allowing imaging of the functional liver mass with high contrast. Thus, our first generation compound resulted already in an alternative to 99mTc-GSA for imaging the functional liver reserve and might allow the broader use of this imaging technique.
Cardiac repolarization abnormalities and neurally mediated syncope: overlooked aspects in the diagnosis of pheochromocytoma
Authors: Cotias Elisama Pimentel Zamian , Neto Jorge Elias , Gomes Luiz Fernando Mazzini , Merçon Erick Sessa , Kuniyoshi Ricardo , Silva Márcio Augusto
Published: May 2024

Background

Pheochromocytoma is a rare catecholamine-producing tumour originating from adrenal chromaffin cells. Classic clinical presentation includes headache, diaphoresis and palpitation. Syncope is uncommon and may result from hypotension or arrhythmia. We report a case of neurally mediated syncope in pheochromocytoma associated with cardiac repolarization abnormalities, a unique presentation of the disease.

Case presentation

This case report presents a 28 year-old woman, describing recurrent syncope usually preceded by prodromes such as headache, visual blurring, heart palpitations and psychomotor agitation. Firstly she was diagnosed with anxiety and depression, but there was no improvement of symptoms with psychiatric treatment. Previous Holter revealed long QT interval. Echocardiogram showed normal dimensions, preserved left ventricular function and no valvopathies. The patient was referred for a Head-up tilt test (HUTT) in order to investigate reflex syncope. The electrocardiogram (ECG) exhibited normal sinus rhythm and diffuse cardiac repolarisation abnormalities. HUTT was positive for vasodepressor type neurally mediated syncope and ECG showed U waves after a syncope episode. This electrocardiographic finding motivated further clinical investigation. The patient also described abdominal pain, increased abdominal volume, weight loss and blood pressure instability. Based on clinical history and ventricular repolarisation abnormalities uncovered by HUTT, pheochromocytoma was suspected. Urinary test was positive for catecholamines and the abdominal imaging revelead expansive lesion. She underwent surgery to remove the pheochromocytoma, confirmed by immunohistochemistry.

Conclusions

After surgery she had no recurrency of symptoms, remaining asymptomatic after six years of follow-up, suggesting a cause-effect relationship between neurally mediated syncope and pheochromocytoma.
V4RIN: visual analysis of regional industry network with domain knowledge
Authors: Xiong Wenli , Yu Chenjie , Shi Chen , Zheng Yaxuan , Wang Xiping , Hu Yanpeng , Yin Hong , Li Chenhui , Wang Changbo
Published: May 2024

The regional industry network (RIN) is a type of financial network derived from industry networks that possess the capability to describe the connections between specific industries within a particular region. For most investors and financial analysts lacking extensive experience, the decision-support information provided by industry networks may be too vague. Conversely, RINs express more detailed and specific industry connections both within and outside the region. As RIN analysis is domain-specific and current financial network analysis tools are designed for generalized analytical tasks and cannot be directly applied to RINs, new visual analysis approaches are needed to enhance information exploration efficiency. In this study, we collaborated with domain experts and proposed V4RIN, an interactive visualization analysis system that integrates predefined domain knowledge and data processing methods to support users in uploading custom data. Through multiple views in the system panel, users can comprehensively explore the structure, geographical distribution, and spatiotemporal variations of the RIN. Two case studies were conducted and a set of expert interviews with five domain experts to validate the usability and reliability of our system.
Sustainable supply chain management and organizational performance: the mediating role of competitive advantage in Ethiopian manufacturing industry
Authors: Shebeshe Ephrem Negash , Sharma Dhiraj
Published: May 2024

Purpose

This research aims to study the impact of sustainable supply chain management on both competitive advantage and organizational performance in the Ethiopian manufacturing industry.

Design/methodology/approach

The objectives are achieved through collecting and analyzing data from 221 Ethiopian manufacturing industries. This research employs a quantitative approach, specifically descriptive and causal research methods. The data are collected by questionnaires administered directly to a sample of 221 respondents who are managers and supervisors in the manufacturing industry. In addition, data analysis was performed using structural equation modeling in the Smart-PLS Software version (SmartPLS 4.0).

Findings

The research reveals that SSCM substantially and positively impacts competitive advantage and organizational performance. Furthermore, statistical findings prove the connection between competitive advantage and organizational performance. Moreover, competitive advantage indirectly influences the relationship between SSCM and OP. The results suggest that successfully implementing SSCM can improve competitive advantage and OP.

Originality/value

Considering the triple-bottom-line approach and the mediating effects of competitive advantage, this study is the first to analyze the relationship between SSCM and manufacturing performance in Ethiopia. This study adds to the existing literature by providing empirical evidence on the impact of sustainable supply chain management (SSCM) on competitive advantage and organizational performance in the manufacturing industry of emerging markets.

Research limitations/implications

The research is based on a cross-sectional study, which may prevent the generalization of findings derived from the current study. The analyzed variable in this study quantified OP, which is widely recognized as a very dynamic concept.
Streaming prediction-error filters
Authors: Fomel Sergey , Claerbout Jon
Published: May 2024

Prediction-error filters (PEFs) are essential for seismic deconvolution and other geophysical estimation problems. We find that nonstationary multidimensional PEFs can be computed in a “streaming” manner, wherein the filter is updated incrementally by accepting one new data point at a time. The computational cost of estimating a streaming PEF is reduced to the cost of a single convolution. In other words, the cost of the PEF design while filtering is equivalent to the cost of applying the filter. Moreover, the nonlinear operation of finding and applying a streaming PEF is invertible at a similar cost, which enables a fast approach for missing data interpolation.
Expert evidence: dangers and the enhancement of reasoning
Authors: Charleton Peter , Rakhmanin Ivan
Published: May 2024

This article considers the role of experts and their interaction with the legal system to better understand the benefits and potential dangers of expert evidence to fact-finders in trials. Medical experts are indispensable to the administration of justice as litigation ranges beyond what judges or juries comfortably deal with as facts of everyday life. This would render courts, absent expert evidence, vastly under-equipped in making decisions of fact. However, the dangers of surrendering authority to experts or of misunderstanding their role must be considered to ensure that expert evidence is used to benefit the administration of justice.
Modified gravity theories from the Barrow hypothesis
Authors: Anand Ankit , Campos Delgado Ruben
Published: May 2024

Barrow proposed that quantum gravity effects might introduce fractal corrections to the area of the event horizon of black holes. The area law gets modified as

, with

. It was so far unclear whether this assumption could lead to meaningful quantum gravity theories beyond general relativity. In this paper, we argue that this is indeed the case. In particular, assuming Δ to be a radial function, we show that the Barrow hypothesis, together with Jacobson's approach can generate non-trivial modified gravity theories.
Dynamics of the nonlinear optical second- and third-harmonic generation in
quantum well due to hydrostatic pressure and temperature
Authors: Yan Xiaolong , Li Xuechao , Cai Yawen
Published: May 2024

We studied the effects of hydrostatic pressure and temperature on the second- and third-harmonic generation (SHG and THG) in semi-parabolic GaAs/Al0.3Ga0.7As quantum wells. By solving the Schrödinger equation numerically, we can determine the system's sub-band energy levels and wave functions, and then calculate its optical gain. The numerical simulation results show that both temperature and hydrostatic pressure can effectively improve the nonlinear optical performance of the system. Notably, we explore the mechanisms underlying these interactions. These findings suggest a viable approach to adjust the nonlinear optical performance of GaAs/Al0.3Ga0.7As quantum system through the subtle interaction between hydrostatic pressure, temperature and structural parameters.