bluuebunny/crossref_metadata_2025 huggingface dataset:
The hybrid bio-robotic swarm as a powerful tool for collective motion research: a perspective
Authors: Ayali Amir , Kaminka Gal A.
Published: July 2023

Swarming or collective motion is ubiquitous in natural systems, and instrumental in many technological applications. Accordingly, research interest in this phenomenon is crossing discipline boundaries. A common major question is that of the intricate interactions between the individual, the group, and the environment. There are, however, major gaps in our understanding of swarming systems, very often due to the theoretical difficulty of relating embodied properties to the physical agents—individual animals or robots. Recently, there has been much progress in exploiting the complementary nature of the two disciplines: biology and robotics. This, unfortunately, is still uncommon in swarm research. Specifically, there are very few examples of joint research programs that investigate multiple biological and synthetic agents concomitantly. Here we present a novel research tool, enabling a unique, tightly integrated, bio-inspired, and robot-assisted study of major questions in swarm collective motion. Utilizing a quintessential model of collective behavior—locust nymphs and our recently developed Nymbots (locust-inspired robots)—we focus on fundamental questions and gaps in the scientific understanding of swarms, providing novel interdisciplinary insights and sharing ideas disciplines. The Nymbot-Locust bio-hybrid swarm enables the investigation of biology hypotheses that would be otherwise difficult, or even impossible to test, and to discover technological insights that might otherwise remain hidden from view.
Modifying functional brain networks in focal epilepsy by manual visceral-osteopathic stimulation of the vagus nerve at the abdomen
Authors: Lehnertz Hendrik , Broehl Timo , Rings Thorsten , von Wrede Randi , Lehnertz Klaus
Published: July 2023

Non-invasive transcutaneous vagus nerve stimulation elicits similar therapeutic effects as invasive vagus nerve stimulation, offering a potential treatment alternative for a wide range of diseases, including epilepsy. Here, we present a novel, non-invasive stimulation of the vagus nerve, which is performed manually viscero-osteopathically on the abdomen (voVNS). We explore the impact of short-term voVNS on various local and global characteristics of EEG-derived, large-scale evolving functional brain networks from a group of 20 subjects with and without epilepsy. We observe differential voVNS-mediated alterations of these characteristics that can be interpreted as a reconfiguration and modification of networks and their stability and robustness properties. Clearly, future studies are necessary to assess the impact of such a non-pharmaceutical intervention on clinical decision-making in the treatment of epilepsy. However, our findings may add to the current discussion on the importance of the gut-brain axis in health and disease.

Clinical Trial Registration: https://drks.de/search/en/trial/DRKS00029914, identifier DRKS00029914
Models and methods: a perspective of the impact of six IMI translational data-centric initiatives for Alzheimer’s disease and other neuropsychiatric disorders
Authors: North Hilary , Hofmann-Apitius Martin , Kas Martien J. H. , Marston Hugh , Haas Magali
Published: July 2023

The Innovative Medicines Initiative (IMI), was a European public–private partnership (PPP) undertaking intended to improve the drug development process, facilitate biomarker development, accelerate clinical trial timelines, improve success rates, and generally increase the competitiveness of European pharmaceutical sector research. Through the IMI, pharmaceutical research interests and the research agenda of the EU are supported by academic partnership and financed by both the pharmaceutical companies and public funds. Since its inception, the IMI has funded dozens of research partnerships focused on solving the core problems that have consistently obstructed the translation of research into clinical success. In this post-mortem review paper, we focus on six research initiatives that tackled foundational challenges of this nature: Aetionomy, EMIF, EPAD, EQIPD, eTRIKS, and PRISM. Several of these initiatives focused on neurodegenerative diseases; we therefore discuss the state of neurodegenerative research both at the start of the IMI and now, and the contributions that IMI partnerships made to progress in the field. Many of the initiatives we review had goals including, but not limited to, the establishment of translational, data-centric initiatives and the implementation of trans-diagnostic approaches that move beyond the candidate disease approach to assess symptom etiology without bias, challenging the construct of disease diagnosis. We discuss the successes of these initiatives, the challenges faced, and the merits and shortcomings of the IMI approach with participating senior scientists for each. Here, we distill their perspectives on the lessons learned, with an aim to positively impact funding policy and approaches in the future.
Case report: “Proust phenomenon” after right posterior cerebral artery occlusion
Authors: De Beukelaer Sophie , Sokolov A. A. , Müri R. M.
Published: July 2023

Odors evoking vivid and intensely felt autobiographical memories are known as the “Proust phenomenon,” delineating the particularity of olfaction in being more effective with eliciting emotional memories than other sensory modalities. The phenomenon has been described extensively in healthy participants as well as in patients during pre-epilepsy surgery evaluation after focal stimulation of the amygdalae and post-traumatic stress disorder (PTSD). In this study, we provide the inaugural description of aversive odor-evoked autobiographical memories after stroke in the right hippocampal, parahippocampal, and thalamic nuclei. As potential underlying neural signatures of the phenomenon, we discuss the disinhibition of limbic circuits and impaired communication between the major networks, such as saliency, central executive, and default mode network.
Case report: Self-restraint in a patient with alien hand syndrome following cerebral infarction involving the anterior cerebral artery territory
Authors: Sugawara Kazuhiro , Takeuchi Toshiki , Harada Kuniaki , Taki Marina , Fujimura Ikumi , Kogami Yuichi , Furuta Ryoichi
Published: July 2023

Frontal alien hand syndrome (AHS) presents as impulsive grasping and groping and compulsive manipulation of environmental objects that can affect the dominant or nondominant hand. A few reports have shown improvements in neuropsychological scores over time when self-restraint of the right hand AHS was enforced. A 72-year-old woman presented with right-handed involuntary instinctive grasping reactions and compulsive manipulation of tools after an infarction of the frontal lobe and corpus callosum (CC). She was diagnosed with cerebral infarction involving the anterior cerebral artery territory and a frontal variant of AHS. At AHS onset, the patient was unaware that her right hand was moving against her will; she was only aware that her right hand was moving when the therapist pointed it out to her. Later, she began to recognize that her right hand was involuntarily moving, and she could restrain the movement of her right hand with her left hand. Approximately 5 months following AHS onset, the patient could voluntarily restrain her AHS symptoms by telling her right hand not to move against her will in her head. Most neuropsychological scores improved by 5 months following AHS onset. However, the patient showed disruptions in the genu and midbody of the left cingulate cortex, as shown via diffusion tensor imaging (DTI), and the sensation of the “right hand moving by itself” remained even 5 months after AHS onset. Although damage to the CC fibers was evident on DTI at 5 months following onset, the patient exhibited no sensory deficits and demonstrated good hand ownership as well as early improvement in attention and cognitive dysfunction. Therefore, the patient recognized her AHS symptoms, which included her hand moving against her will, and was able to consciously restrain her hand movement.
Would frontal midline theta indicate cognitive changes induced by non-invasive brain stimulation? A mini review
Authors: Nakamura-Palacios Ester Miyuki , Falçoni Júnior Aldren Thomazini , Anders Quézia Silva , de Paula Lucas dos Santos Pereira , Zottele Mariana Zamprogno , Ronchete Christiane Furlan , Lirio Pedro Henrique Cassaro
Published: July 2023

To the best of our knowledge, neurophysiological markers indicating changes induced by non-invasive brain stimulation (NIBS) on cognitive performance, especially one of the most investigated under these procedures, working memory (WM), are little known. Here, we will briefly introduce frontal midline theta (FM-theta) oscillation (4–8 Hz) as a possible indicator for NIBS effects on WM processing. Electrophysiological recordings of FM-theta oscillation seem to originate in the medial frontal cortex and the anterior cingulate cortex, but they may be driven more subcortically. FM-theta has been acknowledged to occur during memory and emotion processing, and it has been related to WM and sustained attention. It mainly occurs in the frontal region during a delay period, in which specific information previously shown is no longer perceived and must be manipulated to allow a later (delayed) response and observed in posterior regions during information maintenance. Most NIBS studies investigating effects on cognitive performance have used n-back tasks that mix manipulation and maintenance processes. Thus, if considering FM-theta as a potential neurophysiological indicator for NIBS effects on different WM components, adequate cognitive tasks should be considered to better address the complexity of WM processing. Future research should also evaluate the potential use of FM-theta as an index of the therapeutic effects of NIBS intervention on neuropsychiatric disorders, especially those involving the ventral medial prefrontal cortex and cognitive dysfunctions.
Major phenolic compounds, antioxidant, antimicrobial, and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India
Authors: Srivastava Ravi Prakash , Kumar Sachin , Singh Lav , Madhukar Mayank , Singh Nitesh , Saxena Gauri , Pandey Shivaraman , Singh Arpit , Devkota Hari Prasad , Verma Praveen C. , Shiva Shatrughan , Malik Sumira , Rustagi Sarvesh
Published: July 2023

Antibiotic resistance poses a serious threat to public health, raising the number of diseases in the community. Recent research has shown that plant-derived phenolic compounds have strong antimicrobial, antifungal, and cytotoxic properties against a variety of microorganisms and work as great antioxidants in such treatments. The goal of the current work is to evaluate the anticancerous, antibacterial, antifungal, antioxidant, and cytotoxicity activities in the extracts of the different plant parts (leaves, stems, and roots) ofS. carvifolia(L.) L. This is a medicinally important plant and has been used for different kinds of diseases and ailments such as hysteria and seizures. The phenolic compounds from the different plant parts were analyzed using HPLC and the following were found to be present: chlorogenic acid, gallic acid, rutin, syringic acid, vanillic acid, cinnamic acid, caffeic acid, and protocatechuic acid. Gallic acid was found to have the highest concentration (13.93 mg/g), while chlorogenic acid (0.25 mg/g) had the lowest. The maximum TPC value, which ranged from 33.79 to 57.95 mg GAE/g dry extract weight, was found in the stem. Root extract with 9.4 mg RE/g had the greatest TFC level. In the leaf and stem extracts, the RSC ranged from 0.747 mg/mL to 0.734 mg/1 mL GE/g dry extract weight, respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to measurein vitroantioxidant activity. In a concentration-dependent way, promising antioxidant activity was reported. Moreover, 3,5-dinitrosalicylic acid (DNSA) and the Folin–Ciocalteu phenol reagent technique were used to determine reducing sugar content and total phenolic content, respectively. Antibacterial activity against eight strains (MIC: 250–1,000 μg/mL) was analyzed, and the stem extract exhibited maximum activity. Antifungal activity was also assessed, and potent activity was reported especially in the extract obtained from the stem. Cytotoxicity was evaluated using an MTT assay in the A549 cell line, where different doses (0.0625, 0.125, 0.25, 0.5, and 1 mg/mL) of leaf, root, and stem extracts were used. Treatment with these extracts reduced the cell viability, indicating thatS. carvifoliamay possess anticancer potential, which can be of great therapeutic value.
Arsenic in brown rice: do the benefits outweigh the risks?
Authors: Su Lihchyun Joseph , Chiang Tung-Chin , O’Connor Sarah N.
Published: July 2023

Brown rice has been advocated for as a healthier alternative to white rice. However, the concentration of arsenic and other pesticide contaminants is greater in brown rice than in white. The potential health risks and benefits of consuming more brown rice than white rice remain unclear; thus, mainstream nutritional messaging should not advocate for brown rice over white rice. This mini-review aims to summarize the most salient concepts related to dietary arsenic exposure with emphasis on more recent findings and provide consumers with evidence of both risks and benefits of consuming more brown rice than white rice. Despite risk-benefit assessments being a challenging new frontier in nutrition, researchers should pursue an assessment to validate findings and solidify evidence. In the interim, consumers should be cognizant that the dose of arsenic exposure determines its toxicity, and brown rice contains a greater concentration of arsenic than white rice.
Ginger: a representative material of herb-derived exosome-like nanoparticles
Authors: Zhu He , He Wenxi
Published: July 2023

Edible plant-derived exosome-like nanoparticles (PELNs) provide numerous benefits, including high yield, low cost, ethical compatibility, and multiple health benefits, which enable them to address technical constraints associated with mammalian nanoparticles. Herbs, known for their abundant bioactive components, are considered the primary source of natural medicines within the plant kingdom. Recently, a number of herbaceous sources have been investigated for the isolation and functionality of exosome-like nanoparticles (ELNs). However, they are commonly referred to as PELNs, and their distinct pharmacological properties are overlooked. In this review, these herb-derived ELNs are designated as HELNs, a novel herbal product that may also exhibit superior pharmacological activity compared to other types of PELNs. Among the documented HELNs, ginger-derived exosome-like nanoparticles (GELNs) are the most extensively studied. This review employs GELNs as an exemplar to delineate the process of extraction and purification, together with their physical and biochemical characteristics and therapeutic potential. The aim of this review is to promote the development and application of HELNs, and future research is encouraged to uncover their additional properties, extending beyond those of GELNs.
Prognostic scoring system based on eosinophil- and basophil-related markers for predicting the prognosis of patients with stage II and stage III colorectal cancer: a retrospective cohort study
Authors: Gao Lijing , Yuan Chao , Fu Jinming , Tian Tian , Huang Hao , Zhang Lei , Li Dapeng , Liu Yupeng , Meng Shuhan , Liu Ying , Zhang Yuanyuan , Xu Jing , Jia Chenyang , Zhang Ding , Zheng Ting , Fu Qingzhen , Tan Shiheng , Lan Li , Yang Chao , Zhao Yashuang , Liu Yanlong
Published: July 2023

Background

Systemic inflammation is associated with the prognosis of colorectal cancer (CRC). The current study aimed to construct a comprehensively inflammatory prognostic scoring system named risk score (RS) based on eosinophil- and basophil-related markers and assess its prognostic value in patients with stage II and stage III CRC.

Patients and methods

A total of 3,986 patients were enrolled from January 2007 to December 2013. The last follow-up time was January 2019. They were randomly assigned to the training set and testing set in a 3:2 split ratio. Least absolute shrinkage and selection operator (LASSO)–Cox regression analysis was performed to select the optimal prognostic factors in the construction of RS. The Kaplan–Meier curve, time-dependent receiver operating characteristic (ROC), and Cox analysis were used to evaluate the association between RS and overall survival (OS).

Results

In the training set, all inflammatory markers showed certain prognostic values. Based on LASSO-Cox analysis, nine markers were integrated to construct RS. The Kaplan–Meier curve showed that a higher RS (RS > 0) had a significantly worse prognosis (log-rank p< 0.0001). RS (>0) remained an independent prognostic factor for OS (hazard ratio (HR): 1.70, 95% confidence interval (CI), 1.43–2.03, p< 0.001). The prognostic value of RS was validated in the entire cohort. Time-dependent ROC analysis showed that RS had a stable prognostic effect throughout the follow-up times and could enhance the prognostic ability of the stage by combination. Nomogram was established based on RS and clinicopathological factors for predicting OS in the training set and validated in the testing set. The area under the curve (AUC) values of the 3-year OS in the training and testing sets were 0.748 and 0.720, respectively. The nomogram had a satisfactory predictive accuracy and had better clinical application value than the tumor stage alone.

Conclusions

RS might be an independent prognostic factor for OS in patients with stage II and III CRC, which is helpful for risk stratification of patients. Additionally, the nomogram might be used for personalized prediction and might contribute to formulating a better clinical treatment plan.
The mediating effect of allostatic load on the association between life course socioeconomic disadvantage and chronic pain: a prospective finding from the National Survey of Midlife Development in the United States
Authors: Liang Yunlong
Published: July 2023

Background

Socioeconomic disadvantages (SEDs) are associated with chronic pain (CP) and allostatic load (AL). Few prospective population-based studies have examined the relationship between life course SED, CP interference, and CP widespreadness, and there is no prospective population-based study on whether AL mediates the association between SED and CP.

Objective

In this study, we investigated whether the prospective effect of SED on CP at Midlife in the United States (MIDUS) 3 is consistent with the accumulation of risk model and social mobility model, using the National Survey of MIDUS (n = 593). To prepare for the mediation analysis, we tested (1) whether SED would be prospectively associated with AL in the MIDUS 2 biomarker project, (2) whether AL would be prospectively associated with CP, and (3) whether childhood, as a critical period, moderated the association between AL and CP. In addition, the mediating effect of AL on the association between SED and CP was examined.

Method

SED was measured using cumulative scores and disadvantage trajectories derived from latent class trajectory modeling (LCTM). After multiple imputations, analyses were conducted using multinomial logistic regression for CP and negative binomial regression for AL, respectively. Finally, mediation analyses and moderated mediation analyses were performed.

Results

LCTM identified three SED trajectories, namely, constant low, high to low, and medium to high. The results showed that proximal cumulative SED was associated with high-interference CP. Furthermore, compared with the group with constant low SED, the group with medium-to-high SED was significantly associated with high-interference pain and experienced pain in at least three different sites. Cumulative SED and deteriorating SED trajectories were associated with higher AL, consistent with previous studies. Furthermore, childhood SED moderated the effect of AL on CP widespreadness and unexpectedly demonstrated a protective effect, while other associations between AL and CP were not significant. Subsequent mediation analysis did not yield statistically significant evidence.

Conclusions

People who experienced more recent SED or increasing disadvantage throughout their lives were more likely to suffer from CP, and this association was not mediated by physiological system dysregulation caused by chronic stress. Therefore, measures to alleviate AL may not be effective in protecting socioeconomically disadvantaged populations from CP.
Cleft palate in fetuses: feasibility of early diagnosis by Crystal and Realistic Vue rendering 3D ultrasound technology in the first trimester
Authors: Shi Zhihong , Wen Huaxuan , Leng Junhong , Wang Junjun , Wang Yuemei , Luo Dandan , Chen Zhixuan , Qin Yue , Liang Meiling , Tan Ying , Li Shengli
Published: July 2023

Objectives

This study aimed to evaluate the feasibility of direct visualization of a normal fetal palate and detect cleft palate in the first trimester with a novel three-dimensional ultrasound (3D US) technique, Crystal and Realistic Vue (CRV) rendering technology.

Methods

Two-dimensional (2D) images and 3D volumes of healthy and cleft palate fetuses at 11–13+6 weeks were obtained prospectively. 2D ultrasound views included the coronal view of the retronasal triangle and the midsagittal view of the face. 3D-CRV views were analyzed by multiplanar mode display. The pregnancy outcomes of all fetuses were determined during the follow-up period.

Results

In our study, 124 fetuses were recruited, including 100 healthy fetuses and 24 cleft palate fetuses. The cleft palate with lip was observed in 23 fetuses (bilateral in 15, unilateral in 6, median in 2), and one cleft palate was only found in the abnormal group. The bilateral (n = 12) and median (n = 2) cleft palates with lips and the cleft palate alone (n = 1) were associated with other anatomical or chromosomal abnormalities, and one unilateral cleft palate with cleft lip had concomitant NT thickening. In the cleft palate fetus group, 16 fetuses suffered intrauterine death, which was associated with other structural or chromosomal abnormalities in 14 fetuses, seven cases were terminated after consultation, and one was delivered at term. The coronal view of the retronasal triangle and the midsagittal view was easily obtained in all fetuses. 3D-CRV images of palatal parts were clearly obtained in all cases. Unilateral, bilateral, and median cleft palates with cleft lips were visually demonstrated and classified by the 3D-CRV technique.

Conclusion

It is feasible to identify the palate by 3D-CRV in the first trimester in both healthy and cleft palate fetuses. Together with 2D ultrasonography as a complementary diagnostic tool, 3D-CRV is helpful in classifying the cleft palate with a reasonable degree of certainty.
Case report: Aseptic splenic abscesses in childhood-onset systemic lupus erythematosus
Authors: Sritharan Shobashenee , Lau Peter Sie-Teck , Manan Kamilah , Mohan Anand
Published: July 2023

Background

Systemic lupus erythematosus (SLE) can involve any organ system and cause a wide range of manifestations. Noninfectious inflammatory lesions termed aseptic abscesses have been reported in some autoimmune and autoinflammatory conditions but not in childhood-onset SLE. In this report, we highlight the unusual finding of occult splenic abscesses in two children diagnosed with SLE who had no evidence of concomitant infection.

Case presentation

An 8-year-old and an 11-year-old were admitted separately to the hospital with fever for 7 and 14 days, respectively. In the younger child, a generalized rash preceded the fever. Both had been well, with no significant past medical history prior to the onset of the illness. In both girls, abdominal ultrasonography showed multiple small hypoechoic lesions suggestive of abscesses scattered throughout the spleen. Their C-reactive protein and blood cultures were negative, and symptoms persisted despite intravenous antibiotics. Fulfilling the clinical and immunologic criteria for diagnosis, both were ultimately diagnosed with childhood-onset SLE. Rapid recovery of symptoms and complete resolution of the abscesses ensued with corticosteroids and immunosuppressive therapy.

Conclusions

These two cases suggest that aseptic splenic abscesses may occur in childhood-onset SLE. Autoimmune conditions such as SLE should be included in the differential diagnosis of children with occult splenic abscesses.
Six years’ experience and trends of serum 25-hydroxy vitamin D concentration and the effect of vitamin D3 consumption on these trends
Authors: Horváth László , Mirani Sara , Girgis Michael Magdy Fahmy , Rácz Szilvia , Bácskay Ildikó , Bhattoa Harjit Pal , Tóth Béla E.
Published: July 2023

Introduction: Vitamin D (vitD) deficiency may have importance in some diseases, but there is a lack of data in our country to clarify the current situation. Our aim was to examine the basic characteristics of patients’ vitD status, and the ratio of vitD deficiency and its relation to certain diseases, assess seasonality and trends, and reveal the indirect impact of the COVID-19 pandemic on vitD3 supplementation at the patient population level.

Methods: Anonymized data on 25(OH)D test results were obtained from the clinical data registry of a tertiary teaching hospital covering the period between 1 January 2015 and 30 June 2021. VitD consumption (pharmacy sale) data were retrieved from the database of the National Health Insurance Fund of Hungary in order to calculate the defined daily dose (DDD)/1,000 inhabitants/day. Descriptive statistics and odds ratios with their 95% confidence intervals were calculated. The two-sample t-test and F-test were used to analyze our patients’ data. Significant differences were considered if p <0.05.

Results: Altogether, 45,567 samples were investigated; the mean age was 49 ± 19.1 years and 68.4% of them were female subjects. Overall, 20% of all patients had hypovitaminosis D, and just over 7% of patients had vitD deficiency. Male subjects had higher odds for hypovitaminosis or vitD deficiency (65.4 ± 28.2 nmol/L vs. 68.4 ± 28.4 nmol/L; p <0.0001). The mean 25(OH)D concentration has changed during the year, reaching a peak in September and a minimum in February. Patients with diseases of the circulatory system, genitourinary system, certain conditions originating in the perinatal period, and “sine morbo” (i.e., without a disease; such as those aged over 45 years and female teenagers) had statistically higher odds for lower 25(OH)D concentrations (p <0.00001). VitD consumption showed seasonality, being higher in autumn and winter. A slight increase started in the season of 2017/18, and two huge peaks were detected at the beginning of 2020 and 2021 in association with the COVID-19 waves.

Conclusion: Our data are the first to describe data concerning vitD in our region. It reinforces the notion of vitD3 supplementation for some risk groups and also in healthy individuals. To prevent the winter decline, vitD3 supplementation should be started in September. This and the results during the COVID-19 pandemic highlight the importance of health education encouraging vitamin D3 supplementation.
Abnormal balance control mechanisms during dynamic reaching forward and quiet standing in patients with anterior cruciate ligament reconstruction
Authors: Wang Wei , Li Xudong , Shi Runxiu , Wang Cheng , Zhang Ke , Ren Xiaomin , Wei Hui
Published: July 2023

Purpose: Postural instability and decreased balance control ability have been observed in patients after anterior cruciate ligament (ACL) reconstruction. Herein, we examined the abnormal balance control mechanisms of these patients during dynamic reaching forward and quiet standing, providing a quantitative index for rehabilitation assessment.

Methods: We enrolled ACL reconstruction patients 6–8 months after surgery, and 14 gender- and age-matched healthy volunteers. The IKDC and Lysholm were applied in each patient after ACL reconstruction. All participants conducted the quiet standing and reaching forward (RF) tests at the specified locations on force plates. The ground reaction force, center of pressure (COP), and kinematics signals were recorded. The maximal reach distance (MRD), speed of RF, length of COP, peak speed of COP in anterior-posterior direction (AP-COP), and weight bearing ratio (WBR) of the affected limb were calculated in the RF test. The COP speed, COP amplitude, frequency components of COP and WBR were extracted during quiet standing.

Results: We observed that the speed of RF in the patients after ACL reconstruction was significantly lower than that of controls (p < 0.05). The COP length during RF was positively correlated with the Lysholm scale in the affected limb of patients (r = 0.604, p < 0.05). The peak of AP-COP speed during RF in the affected limb of patients was significantly lower than that of the healthy controls (p < 0.05), and positively correlated with the IKDC scale (r = 0.651, p < 0.05). WBR on the affected limb of patients during RF were significantly lower than that of controls (p < 0.05). The mean (r = −0.633, p < 0.05) and peak (r = −0.643, p < 0.05) speeds of COP during quiet standing were negatively correlated with the IKDC scale value. The amplitude of AP-COP on the contralateral side of patients was significantly higher than that of controls during quiet standing (p < 0.05).

Conclusion: Patients after ACL reconstruction performed decreased postural control capacity, especially in dynamic balance, and were accompanied by deficiencies in proprioception. The COP length, peak speed of COP during RF and COP speed during quiet standing could be considered as quantitative index of balance function assessment after ACL reconstruction.
Preterm pigs for preterm birth research: reasonably feasible
Authors: Sun Jing , Chong Jie , Zhang Jinwei , Ge Liangpeng
Published: July 2023

Preterm birth will disrupt the pattern and course of organ development, which may result in morbidity and mortality of newborn infants. Large animal models are crucial resources for developing novel, credible, and effective treatments for preterm infants. This review summarizes the classification, definition, and prevalence of preterm birth, and analyzes the relationship between the predicted animal days and one human year in the most widely used animal models (mice, rats, rabbits, sheep, and pigs) for preterm birth studies. After that, the physiological characteristics of preterm pig models at different gestational ages are described in more detail, including birth weight, body temperature, brain development, cardiovascular system development, respiratory, digestive, and immune system development, kidney development, and blood constituents. Studies on postnatal development and adaptation of preterm pig models of different gestational ages will help to determine the physiological basis for survival and development of very preterm, middle preterm, and late preterm newborns, and will also aid in the study and accurate optimization of feeding conditions, diet- or drug-related interventions for preterm neonates. Finally, this review summarizes several accepted pediatric applications of preterm pig models in nutritional fortification, necrotizing enterocolitis, neonatal encephalopathy and hypothermia intervention, mechanical ventilation, and oxygen therapy for preterm infants.
Higher education students perceptions of NNESTs’ language proficiency: will it affect their learning effectiveness?
Authors: Bing Xiaoyi , Gao Xiaoqing , Yang Zhen
Published: July 2023

Increasingly, higher education institutions are giving more attention to the language proficiency of non-native English-speaking teachers (NNESTs) due to their growing numbers. Despite a recent surge in the literature on NNESTs in the global discourse of English language teaching (ELT), the impacts of NNESTs’ language competency within the higher education systems of their countries remain woefully under-examined. Of particular concern is the absence of students’ voices. Therefore, this study explores higher education students’ perception of NNESTs’ language proficiency. Data was collected through class observations of five NNESTs and followed-up semi-structured interviews with five student focus groups recruited randomly from each class. Our results show that while students concur that NNESTs’ language proficiency contributes to their learning performance in class, other factors (e.g., the teacher’s effective teaching style and charming personality, relaxed class atmosphere, the difficulty level of the teaching materials, and the learners’ proficiency level) also perceived to play key roles in boosting students’ class learning effectiveness. The findings highlight the need to include students in the design of teaching approaches, course design, and curricula, as well as the reflection process about NNESTs’ language proficiency.
The influence of a color themed HMI on trust and take-over performance in automated vehicles
Authors: el Jouhri Aboubakr , el Sharkawy Ashraf , Paksoy Hakan , Youssif Omar , He Xiaolin , Kim Soyeon , Happee Riender
Published: July 2023

Introduction

SAE Level 3 is known as conditional driving automation. As long as certain conditions are met, there is no need to supervise the technology and the driver can engage in non-driving related tasks (NDRTs). However, a human driver must be present and alert to take over when the automation is facing its system limits. When such an emergency takes place, the automation uses the human machine interface (HMI) to send a take-over request (TOR) to the driver.

Methods

We investigated the influence of a color themed HMI on the trust and take-over performance in automated vehicles. Using a driving simulator, we tested 45 participants divided in three groups with a baseline auditory HMI and two advanced color themed HMIs consisting of a display and ambient lighting with the colors red and blue. Trust in automation was assessed using questionnaires while take-over performance was assessed through response time and success rate.

Results

Compared to the baseline HMI, the color themed HMI is more trustworthy, and participants understood their driving tasks better. Results show that the color themed HMI is perceived as more pleasant compared to the baseline HMI and leads to shorter reaction times. Red ambient lighting is seen as more urging than blue, but HMI color did not significantly affect the general HMI perception and TOR performance.

Discussion

Further research can explore the use of color and other modalities to express varying urgency levels and validate findings in complex on road driving conditions.
Comparison of false positive and false negative rates of two indices of individual reliable change: Jacobson-Truax and Hageman-Arrindell methods
Authors: Ferrer-Urbina Rodrigo , Pardo Antonio , Arrindell Willem A. , Puddu-Gallardo Giannina
Published: July 2023

Background

Quantification of change is crucial for correctly estimating the effect of a treatment and for distinguishing random or non-systematic changes from substantive changes. The objective of the present study was to learn about the performance of two distribution-based methods [the Jacobson-Truax Reliable Change Index (RCI) and the Hageman-Arrindell (HA) approach] that were designed for evaluating individual reliable change.

Methods

A pre-post design was simulated with the purpose to evaluate the false positive and false negative rates of RCI and HA methods. In this design, a first measurement is obtained before treatment and a second measurement is obtained after treatment, in the same group of subjects.

Results

In relation to the rate of false positives, only the HA statistic provided acceptable results. Regarding the rate of false negatives, both statistics offered similar results, and both could claim to offer acceptable rates when Ferguson’s stringent criteria were used to define effect sizes as opposed to when the conventional criteria advanced by Cohen were employed.

Conclusion

Since the HA statistic appeared to be a better option than the RCI statistic, we have developed and presented an Excel macro so that the greater complexity of calculating HA would not represent an obstacle for the non-expert user.
Dynamics of organizational climate and job satisfaction in healthcare service practice and research: a protocol for a systematic review
Authors: Santana Silvina , Pérez-Rico Cristina
Published: July 2023

Organizational climate and job satisfaction have been established as fundamental pillars of research and practice in organizational behavior and organizational psychology, inspiring many explanations and operationalizations over time. In most sectors, global trends such as labor shortages, high rates of turnover and absenteeism, the need to increase productivity, and the interest in new work models concur to keep climate and job satisfaction on top of the research agenda. The situation is particularly acute in the healthcare sector, where related factors have the capacity to influence all aspects of care provision, including patient safety and the physical and mental health of care providers. Nevertheless, a gap in knowledge persists regarding climate, job satisfaction, and their relationships in healthcare services. This protocol describes a study that aims to examine the dynamics of climate and job satisfaction in healthcare organizations from the practice and research perspectives. The protocol complies with PRISMA-P. PRISMA will be used to report the results of the study. Databases will be searched for published studies in May 2023, and we expect to complete the study by December 2024. A framework based on a multi-dimensional concept of quality in research will be used to examine the quality of any studies before inclusion. The results will be disseminated in two systematic reviews. We will describe proposed models depicting the dynamics of climate and job satisfaction in healthcare organizations. We will systematize and discuss available evidence regarding the outcomes of climate and job satisfaction in healthcare work environments. We will synthesize information on research designs and methodological options of included studies. We will identify measures of climate and job satisfaction used in healthcare settings, assess their psychometric properties, and appraise the overall quality of underlying studies. Finally, we expect to identify areas in need of further research.
The use of whole-genome sequencing and development of bioinformatics to monitor overlapping outbreaks of Candida auris in southern Nevada
Authors: Gorzalski Andrew , Ambrosio Frank J. , Massic Lauryn , Scribner Michelle R. , Siao Danielle Denise , Hua Chi , Dykema Phillip , Schneider Emily , Njoku Chidinma , Libuit Kevin , Sevinsky Joel R. , Van Hooser Stephanie , Pandori Mark , Hess David
Published: July 2023

ACandida aurisoutbreak has been ongoing in Southern Nevada since August 2021. In this manuscript we describe the sequencing of over 200C. aurisisolates from patients at several facilities. Genetically distinct subgroups ofC. auriswere detected from Clade I (3 distinct lineages) and III (1 lineage). Open-source bioinformatic tools were developed and implemented to aid in the epidemiological investigation. The work herein compares three methods forC. auriswhole genome analysis: Nullarbor, MycoSNP and a new pipeline TheiaEuk. We also describe a novel analysis method focused on elucidating phylogenetic linkages between isolates within an ongoing outbreak. Moreover, this study places the ongoing outbreaks in a global context utilizing existing sequences provided worldwide. Lastly, we describe how the generated results were communicated to the epidemiologists and infection control to generate public health interventions.
Effects of ozone exposure on lipid metabolism in Huh-7 human hepatoma cells
Authors: Peng Jianhao , Wang Siyuan , Wang Yunlong , Yu Wanchao , Zha Yejun , Gao Shuxin
Published: July 2023

Ozone pollution is a major environmental concern. According to recent epidemiological studies, ozone exposure increases the risk of metabolic liver disease. However, studies on the mechanisms underlying the effects of ozone exposure on hepatic oxidative damage, lipid synthesis, and catabolism are limited. In this study, Huh-7 human hepatocellular carcinoma cells were randomly divided into five groups and exposed to 200 ppb O3 for 0, 1, 2, 4, and 8 h. We measured the levels of oxidative stress and analyzed the changes in molecules related to lipid metabolism. The levels of oxidative stress were found to be significantly elevated in Huh-7 hepatocellular carcinoma cells after O3 exposure. Moreover, the expression levels of intracellular lipid synthases, including SREBP1, FASN, SCD1, and ACC1, were enhanced. Lipolytic enzymes, including ATGL and HSL, and the mitochondrial fatty acid oxidase, CPT1α, were inhibited after O3 exposure. In addition, short O3 exposure enhanced the expression of the intracellular peroxisomal fatty acid β-oxidase, ACOX1; however, its expression decreased adaptively with longer exposure times. Overall, O3 exposure induces an increase in intracellular oxidative stress and disrupts the normal metabolism of lipids in hepatocytes, leading to intracellular lipid accumulation.
Development and validation of a prediction model for mechanical ventilation based on comorbidities in hospitalized patients with COVID-19
Authors: Zhang Yi , Zhu Yang-Jie , Zhu Dao-Jun , Yu Bo-Yang , Liu Tong-Tong , Wang Lu-Yao , Zhang Lu-Lu
Published: July 2023

Background

Timely recognition of respiratory failure and the need for mechanical ventilation is crucial in managing patients with coronavirus disease 2019 (COVID-19) and reducing hospital mortality rate. A risk stratification tool could assist to avoid clinical deterioration of patients with COVID-19 and optimize allocation of scarce resources. Therefore, we aimed to develop a prediction model for early identification of patients with COVID-19 who may require mechanical ventilation.

Methods

We included patients with COVID-19 hospitalized in United States. Demographic and clinical data were extracted from the records of the Healthcare Cost and Utilization Project State Inpatient Database in 2020. Model construction involved the use of the least absolute shrinkage and selection operator and multivariable logistic regression. The model’s performance was evaluated based on discrimination, calibration, and clinical utility.

Results

The training set comprised 73,957 patients (5,971 requiring mechanical ventilation), whereas the validation set included 10,428 (887 requiring mechanical ventilation). The prediction model incorporating age, sex, and 11 other comorbidities (deficiency anemias, congestive heart failure, coagulopathy, dementia, diabetes with chronic complications, complicated hypertension, neurological disorders unaffecting movement, obesity, pulmonary circulation disease, severe renal failure, and weight loss) demonstrated moderate discrimination (area under the curve, 0.715; 95% confidence interval, 0.709–0.722), good calibration (Brier score = 0.070, slope = 1, intercept = 0) and a clinical net benefit with a threshold probability ranged from 2 to 34% in the training set. Similar model’s performances were observed in the validation set.

Conclusion

A robust prognostic model utilizing readily available predictors at hospital admission was developed for the early identification of patients with COVID-19 who may require mechanical ventilation. Application of this model could support clinical decision-making to optimize patient management and resource allocation.
Applying soft matter techniques to solve challenges in cryopreservation
Authors: Bryant Saffron J. , Elbourne Aaron , Greaves Tamar L. , Bryant Gary
Published: July 2023

Cryopreservation allows the long-term storage of biological material, and has become integral for numerous applications including assisted reproductive technologies, stem cell therapies, blood banking, and species preservation. However, there are still hundreds of cell types that cannot be cryopreserved, and no organs at all. The biggest challenge facing cryopreservation is the ongoing reliance on predominantly just two cryoprotectants (dimethylsulfoxide and glycerol) as well as physical limitations to heating and cooling rates. The field of soft matter can accelerate cryopreservation research by providing insights into the underlying mechanisms and interactions of cells, cryoprotectants, and solvents including the role of temperature. With techniques as far ranging as differential scanning calorimetry, atomic force microscopy, and neutron and X-ray scattering, it is possible to probe multiple length and time scales in order to identify and characterise important interactions. This in turn can lead to the development of new cryoprotectants, and new methods of heating/cooling which could overcome some of the current challenges of cryopreservation.
AI-ready data in space science and solar physics: problems, mitigation and action plan
Authors: Poduval Bala , McPherron R. L. , Walker R. , Himes M. D. , Pitman K. M. , Azari A. R. , Shneider C. , Tiwari A. K. , Kapali S. , Bruno G. , Georgoulis M. K. , Verkhoglyadova O. , Borovsky J. E. , Lapenta G. , Liu J. , Alberti T. , Wintoft P. , Wing S.
Published: July 2023

In the domain of space science, numerous ground-based and space-borne data of various phenomena have been accumulating rapidly, making analysis and scientific interpretation challenging. However, recent trends in the application of artificial intelligence (AI) have been shown to be promising in the extraction of information or knowledge discovery from these extensive data sets. Coincidentally, preparing these data for use as inputs to the AI algorithms, referred to as AI-readiness, is one of the outstanding challenges in leveraging AI in space science. Preparation of AI-ready data includes, among other aspects: 1) collection (accessing and downloading) of appropriate data representing the various physical parameters associated with the phenomena under study from different repositories; 2) addressing data formats such as conversion from one format to another, data gaps, quality flags and labeling; 3) standardizing metadata and keywords in accordance with NASA archive requirements or other defined standards; 4) processing of raw data such as data normalization, detrending, and data modeling; and 5) documentation of technical aspects such as processing steps, operational assumptions, uncertainties, and instrument profiles. Making all existing data AI-ready within a decade is impractical and data from future missions and investigations exacerbates this. This reveals the urgency to set the standards and start implementing them now. This article presents our perspective on the AI-readiness of space science data and mitigation strategies including definition of AI-readiness for AI applications; prioritization of data sets, storage, and accessibility; and identifying the responsible entity (agencies, private sector, or funded individuals) to undertake the task.