bluuebunny/crossref_metadata_2025 huggingface dataset:
The interaction between Xanthan Gum and Bovine Serum Albumin was studied by Multispectral Method and Molecular Docking Simulation
Authors: sun jisheng , wang xiaoxia , nie zhihua , ma litong , sai huazheng , cheng jianguo , liu yunying , duan jianguo
Published: October 2023

Xanthan gum (XG) is a kind of biological macromolecular drug, which is widely used in the field of biomedicine. Bovine serum albumin is very similar to human serum albumin, and it is easy to extract and cheap, so it is often used to study the interaction with drugs. However, due to the structural complexity of xanthan gum, the interaction of xanthan gum with bovine serum albumin (BSA) and its effects on its secondary structure and mechanism are less studied.In this paper, a variety of spectroscopy and molecular docking will be used to systematically study the interaction mechanism of XG and BSA. On this basis, the interaction and quenching mode between XG and BSA were determined. The intrinsic fluorescence which can effectively quench BSA is obtained by fluorescence spectrum analysis, and the dynamic quenching rate at different temperatures is much higher than the maximum diffusion collision quenching constant, so it can be known that XG interacts with BSA. The quenching constant is solved by Stern-Volmer equation. When the temperature is different, With the increase of temperature, the quenching constant of XG and BSA system decreases, so it can be judged that the quenching method of XG on BSA is static quenching. According to the Vant's Hoff equation, the thermodynamic parameters Δ H < 0 and Δ S < 0 between XG and BSA are mainly hydrogen bond and van der Waals force. If Δ G < 0 and Δ H < 0, it is proved that the quenching process of XG and BSA is spontaneous and exothermic. The molecular docking simulation results show that hydrogen bond and van der Waals force are the main forces between XG and BSA, which is consistent with thermodynamic results, indicating that XG changes the secondary structure of BSA.Through the multi-spectral analysis, it is known that due to the influence of the microenvironment, the polarity and hydrophilicity of XG and BSA are enhanced, and the hydrophobicity is weakened, resulting in changes in the secondary structure of BSA. In the binding distance between XG and BSA, there is an overlap between the fluorescence emission spectrum of BSA and the UV-vis absorption spectrum of XG, which proves that there is energy transfer between BSA and XG, and then the overlap integral is calculated that r is significantly smaller than 7nm, this indicates that there is non-radiative energy transfer between XG and BSA. The results of circular dichroism spectra show that BSA is mainly β-fold. indicating that XG interacts with BSA, and some proteins are loosened, resulting in the decrease of α-Helix structure and the increase of β-sheet structure, which further proves that the secondary structure of BSA has changed. Through the study of the interaction between XG and BSA, the interaction mechanism of both is analyzed by spectroscopy and molecular docking simulation technology, which provides data support for their future discussion and research.
Detection of genes mutations in cerebrospinal fluid circulating tumor DNA from central nervous system lymphomas patients using next generation sequencing
Authors: Fu Haiyin , Wang TaiQin , Yang Yong , Qiu Chenxi , Wang He , Qiu Yanyan , Liu Jianzhi , Liu Tingbo
Published: October 2023

Purpose
To evaluate the potential clinical value of cerebrospinal fluid (CSF) circulating tumor DNA (ctDNA) in the diagnosis and monitors the central nervous system (CNS) lymphomas.
Methods
This was a prospective study of 17 consecutive patients with B-cell lymphoma: 10 patients with CNS lymphomas and 7 patients with B-cell lymphomas at high clinical risk of CNS relapse. Genomic profiles were performed on the CSF and plasma samples of patients by next generation sequencing (NGS).
Results
In patients with CNS lymphomas, ctDNA was detected in 70.0% of CSF and 60.0% of plasma. The detection rate and gene mutation abundance of cerebrospinal fluid were higher than plasma (P=0.016). CSF had a unique genetic profile. Furthermore, we newly found that gene mutations consistent with plasma or lymphoma-related were also detected in the CSF of the high-risk group without CNS involvement. Analysis of paired plasma and CSF samples from three patients at different time points, changes of CSF ctDNA abundance occurred at the same time or earlier than clinical disease changes, which could timely monitor the therapeutic response and relapse trend.
Conclusions
The detection rate of ctDNA in CSF is higher than that in plasma. The dynamic monitoring of ctDNA in CSF has hint significance for therapeutic response of CNS lymphoma patients.
Bioinformatics analysis of differentially expressed genes and pathways in the development of Juvenile- Onset Recurrent Respiratory Papillomatosis
Authors: Nuerbolati Meili , Qukuerhan Ayiheng
Published: October 2023

Background. Juvenile-Onset Recurrent Respiratory Papillomatosis (JORRP) is the most common form of benign respiratory tract tumor in children that characterized by high recurrence and aggressiveness. The purpose of this study was to screen the key genes and pathways of JORRP by biological information analysis and to investigate the molecular mechanisms of JORRP progression.
Methods. RNA sequencing (RNA-seq) was used to identify differentially expressed genes (DEGs) in three JORRP tissues and two adjacent non-tumor tissues, which were first analyzed for gene ontology (GO) and Kyoto Gene and Genome Encyclopedia (KEGG) pathway enrichment.The protein-protein interaction (PPI) network of these DEGs was next constructed with STRING and visualized with Cytoscape software. The central genes were subsequently screened using CytoHubba, a plug-in in Cytoscape software, and finally evaluated using gene function enrichment analysis.
Results. A total of 1795 DEGs were screened, including 876 up-regulated genes and 919 down-regulated genes. The up-regulated DEGs were mainly enriched in cornification, cell cycle, steroid biosynthesis and HIF-1 signaling pathway, and the down-regulated DEGs were associated with cilium movement, cell projection organization, metabolism of xenobiotics by cytochrome P450 and chemical carcinogenesis. VEGFA, CDH1, CDK1, IL1B, KIF11, HIF1A, ASPM, AURKA, CENPE, MKI67 were identified as hub genes from the PPI network, and these genes were mainly associated with spindle organization, cancers-related pathways, Th17 cell differentiation and HIF-1 signaling pathway.
Conclusion. The key genes and pathways screened in this study may play a pivotal role in the development of JORRP and provide a scientific basis for future related studies.
A time-dependent momentum-resolved scattering approach to core-level spectroscopies
Authors: Zawadzki Krissia , Nocera Alberto , Feiguin Adrian
Published: October 2023

While new light sources allow for unprecedented resolution in experiments with X-rays, a theoretical understanding of the scattering cross-section is lacking. In the particular case of strongly correlated electron systems, numerical techniques are quite limited, since conventional approaches rely on calculating a response function (Kramers-Heisenberg formula) that is obtained from a perturbative analysis of scattering processes in the frequency domain. This requires a knowledge of a full set of eigenstates in order to account for all intermediate processes away from equilibrium, limiting the applicability to small tractable systems. In this work, we present an alternative paradigm, recasting the problem in the time domain and explicitly solving the time-dependent Schrödinger equation without the limitations of perturbation theory: a faithful simulation of the scattering processes taking place in actual experiments, including photons and core electrons. We show how this approach can yield the full time and momentum resolved Resonant Inelastic X-Ray Scattering (RIXS) spectrum of strongly interacting many-body systems. We demonstrate the formalism with an application to Mott insulating Hubbard chains using the time-dependent density matrix renormalization group method, which does not require a priory knowledge of the eigenstates and can solve very large systems with dozens of orbitals. This approach can readily be applied to systems out of equilibrium without modification and generalized to other spectroscopies.
Remarks on Berry connection in QFT, anomalies, and applications
Authors: Dedushenko Mykola
Published: October 2023

Berry connection has been recently generalized to higher-dimensional QFT, where it can be thought of as a topological term in the effective action for background couplings. Via the inflow, this term corresponds to the boundary anomaly in the space of couplings, another notion recently introduced in the literature. In this note we address the question of whether the old-fashioned Berry connection (for time-dependent couplings) still makes sense in a QFT on \Sigma^{(d)}× \mathbb{R}Σ(d)×ℝ, where \Sigma^{(d)}Σ(d) is a dd-dimensional compact space and \mathbb{R}ℝ is time. Compactness of \Sigma^{(d)}Σ(d) relieves us of the IR divergences, so we only have to address the UV issues. We describe a number of cases when the Berry connection is well defined (which includes the tt^*tt* equations), and when it is not. We also mention a relation to the boundary anomalies and boundary states on the Euclidean \Sigma^{(d)} × \mathbb{R}_{≥ 0}Σ(d)×ℝ≥0. We then work out the examples of a free 3D Dirac fermion and a 3D \mathcal{N}=2𝒩=2 chiral multiplet. Finally, we consider 3D theories on \mathbb{T}^2× \mathbb{R}𝕋2×ℝ, where the space \mathbb{T}^2𝕋2 is a two-torus, and apply our machinery to clarify some aspects of the relation between 3D SUSY vacua and elliptic cohomology. We also comment on the generalization to higher genus.
What's anomalous in LHC jets?
Authors: Buss Thorsten , Dillon Barry M. , Finke Thorben , Krämer Michael , Morandini Alessandro , Mück Alexander , Oleksiyuk Ivan , Plehn Tilman
Published: October 2023

Searches for anomalies are a significant motivation for the LHC and help define key analysis steps, including triggers. We discuss specific examples how LHC anomalies can be defined through probability density estimates, evaluated in a physics space or in an appropriate neural network latent space, and discuss the model-dependence in choosing an appropriate data parameterisation. We illustrate this for classical k-means clustering, a Dirichlet variational autoencoder, and invertible neural networks. For two especially challenging scenarios of jets from a dark sector we evaluate the strengths and limitations of each method.
Whey permeate powder: microbiological points during different stages of production
Authors: Paveleva D. , Bogdanova Ekaterina , Rudnichenko E. , Melnikova E.
Published: October 2023

Changes in the qualitative and quantitative composition of the microflora of demineralized whey permeate at various stages of the technological process after ultrafiltration, nanofiltration, electrodialysis and spray drying have been investigated. The experiments were conducted in accordance with arbitration and generally accepted methods in research practice. The chemical composition and microbiological parameters of the initial whey, its concentrate and permeate have been established. The technological process of obtaining permeate made it possible to reduce the number of microorganisms by more than 90 %. Bacterial contamination decreases mainly at the stages of whey pasteurization (92 %) and ultrafiltration fractionation (80 %). This technology makes it possible to obtain a standard product with quality and safety indicators corresponding to the Codex Alimentarius CXS 331–2017.
Development of dairy farming in the leading regions of the Siberian Federal District
Authors: Kudinova Margarita , Udovik E. , Suray N. , Nosov V.
Published: October 2023

A set of indicators of milk production in the Siberian Federal District is considered. Data are given for the leading regions of the district (Altai Territory, Novosibirsk Region, Omsk Region, Krasnoyarsk Territory), as well as Irkutsk Region. A comparative analysis of the development of dairy farming in certain regions of the Siberian Federal District showed that dairy farming in Siberia is one of the most important elements in the structure of the agro-industrial complex and makes a significant contribution to the development of Russian agriculture. Despite the reduction in the number of cows, there is an increase in the productivity of the dairy herd.
Concentrated milk model systems: quality changes during low-temperature storage All-Russian Dairy Research Institute
Authors: Tolmachev V. , Ryabova A.
Published: October 2023

One of the ways to solve the problem of low storability of raw milk is the use of frozen milk concentrates. A comprehensive understanding of the impact of negative temperatures on the stability and properties of dairy systems during long-term storage and further processing is necessary to assess the potential of their use. The effect of long-term low-temperature storage on the organoleptic, physico-chemical and technological properties of concentrated model milk systems has been studied. Stability of milk systems samples was kept for 3 months, further significant stratification of consistence and formation of protein flakes were registered. Mass fractions of protein, fat, lactose, as well as titratable and active acidity did not change significantly. On the alcohol and heat samples significant changes were noted only after 6 months of storage. Based on the data obtained, the duration of storage after freezing milk systems should not exceed 3 months.
Development of enriched thick yogurt
Authors: Dunchenko N. , Rashed V. , Yankovskaya V.
Published: October 2023

The possibility of producing thick yoghurts by adding skimmed milk powder in various concentrations and different types of garlic to fermented milk has been studied. The dose of skimmed milk powder was determined, which is necessary to ensure the structural-mechanical and organoleptic characteristics of thick yogurt. The choice of garlic as a source of functional food ingredients for the production of thick yoghurt with flavorings is substantiated. The structural-mechanical, physico-chemical and organoleptic properties most characteristic of thick yogurt were achieved with the addition of 0,5 % garlic powder.
Influence of whey protein hydrolysate on surface- active and foaming properties of curd whey
Authors: Novokshanova A.
Published: October 2023

The surface-active and foaming properties of systems containing 100 cm3 of curd whey and from 1 to 3 g of milk whey protein hydrolysate with a degree of hydrolysis of 60 % were studied. The control was whey samples without hydrolysate. The mass fractions of protein, fat, lactose and solids, active acidity, viscosity and surface tension of the samples were studied by instrument methods. The parameters of serum foaming with hydrolysate were studied by whipping 100 cm3 samples at a speed of 10,000 rpm for 10 seconds in a cylinder. When adding hydrolysate, a tendency was observed to decrease the active acidity and increase the viscosity of the whey without a noticeable change in the surface tension of the prototypes. The addition of up to 3 % hydrolysate of whey proteins to the curd whey of a deep degree of hydrolysis of peptide bonds (60 %) had a positive effect on the surfactant and foaming properties of the serum. The observed effects are primarily due to an increase in the mass fraction of whey proteins that perform the function of surfactants in these systems.
Research of methods of DNA extraction from raw milk
Authors: Lazareva E. , Khan A. , Fomenko O.
Published: October 2023

This article presents the results of work on the study of the effectiveness of various methods of DNA isolation for use in the analysis of biosafety of milk and dairy products by PCR. Some components of milk, such as proteases and calcium ions, can significantly reduce the effectiveness of PCR and lead to distortion of the results. We have given quantitative (DNA concentration) and qualitative assessments (amplification followed by gel electrophoresis) to commercial DNA purification kits «DNA-sorb-S-M» and «DNA-Extran-2». The results obtained indicate the applicability of the selected kits for the isolation of deoxyribonucleic acids from raw milk, the quality and quantity of which allows them to be used for the successful subsequent molecular genetic analysis of the safety and authenticity of milk.
Biological properties of L. helveticus strains
Authors: Leonova V. , Begunova A.
Published: October 2023

The biological properties of three strains of L. helvetcus were studied. All studied strains metabolize D-galactose. The L. helveticus AB strain utilizes D-glucose, D-fructose, N-acetylglucosamine, and D-trehalose; L. helveticus 20T — D-mannose, N-acetylglucosamine; L. helveticus H-9 — D-glucose, D-lactose, D-trehalose. All strains showed high β-galactosidase activity, as well as the activity of acid phosphatase, naphthol-AS-BI hydrolase, leucine arylamidase, and cystine arylamidase. For L. helveticus 20T and L. helveticus AB strains, valine arylamidase activity was found. All strains are sensitive to gentamicin, ampicillin, tetracycline, azithromycin, lincomycin, chloramphenicol. The data obtained indicate the safety of using strains as part of starter cultures and as producers of metabolite additives in terms of reducing the risk of spreading antibiotic resistance.
Overcoming Crises of 2008 and 2020 by Developed and Developing Countries: Comparative Analysis
Authors: Alekhina Olga , Plyasova Svetlana , Yazykova Svetlana , Fedotova Elena
Published: October 2023

Today the socio-economic development suffers from a high level of uncertainty and a large number of global changes. The purpose of the study is to compare how modern crises affected on the economies of developed and developing countries and to assess economies’ weak points and capabilities to recover. The authors analyzed the dynamics of developed and developing countries’ real gross domestic ptoduct in 2001–2021 and identified the 2 most significant crisis periods. A comparative analysis of inflation rates shows that during the pandemic inflation growth was more gradual than in 2008; more restrained in developed countries than in developing countries. A comparative analysis of gross domestic ptoduct level indicators in 2007–2021 in the developed countries, G7 countries and developing countries substantiates that the COVID-19 pandemic crisis led to less serious consequences for the world economy than the global economic crisis of 2008 due to the population and business support programs, implementated by state institutions on an unprecedented scale. According to the analyzed indicators, developing countries’ reaction to crises is more restrained, recovery in the post-crisis period is faster. However, the primary reason for this is the implementation of the global rescue program for developing economies by developed countries. The article presents recommendations on ensuring the post-pandemic recovery of the economies of developed and developing countries.
Analysis of urban water dissipation characteristics considering anthropogenic impacts: a case study in Beijing
Authors: Liu Chuang , Liu Jiahong , Ding Xiangyi , Shao Weiwei , Su Xin
Published: October 2023

Urban water dissipation is a significant part of the urban hydrologic cycle and has a typical natural–social dualistic attribute. Besides natural evaporation, the water dissipation in people's daily life and production process cannot be ignored. This study developed an urban water dissipation model based on different land uses and applied it in urban-built areas in Beijing. The results showed that the water dissipation of buildings and green spaces occupied the dominant position, and the water dissipation intensity of each district exceeded 500 mm, among which the six core districts were 700–1,100 mm. Comparing the water dissipation contribution rate and area rate of each underlying surface, it showed that the water dissipation intensity from strong to weak was building, water surface, green spaces, and hardened ground. According to the dualistic analysis of urban water dissipation, the contribution rates of social water dissipation in the six core districts were 45.3–69.1%, which was higher than the 17.8–36.1% of other suburbs obviously. This study reflected that the higher the degree of regional urbanization, the greater the water dissipation intensity, and artificial water dissipation was the main influencing factor.
Assessment of neurophysiological parameters of the nervous system in non-ferrous foundry workers
Authors:   Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov St., Yekaterinburg, 620014, Russian Federation, Bakhtereva E.V. , Leiderman E.L. ,   Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov St., Yekaterinburg, 620014, Russian Federation, Plotko E.G. ,   Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov St., Yekaterinburg, 620014, Russian Federation, Riabkova T.A. ,   Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov St., Yekaterinburg, 620014, Russian Federation
Published: October 2023

A comprehensive assessment of the health status includes the functional exploration of the central and peripheral nervous systems. Results of the neurophysiological examination allow elaboration of effective personified therapeutic and preventive programs for the core personnel of non-ferrous metal industry. Our objective was to study functional disorders of the central and peripheral nervous systems in smelter operators for further development of risk-based rehabilitation programs for workers engaged in production of non-ferrous metals. Two cohorts of male workers were examined. The case cohort included 60 smelter operators of a large metallurgical plant situated in the Sverdlovsk Region and the control cohort consisted of 50 unexposed employees. The cohorts were matched by age and years of work experience. The mean age of smelter operators was 37.8 ± 7.9 years and their mean length of current employment was 4.1 ± 4.6 years, while the total length of work under hazardous occupational conditions was 7.1 ± 6.0 years. The case cohort included 39 operators of refinery boilers (mean age: 35.6 ± 7.2 years, mean length of employment: 4.2 ± 4.7 years) and 21 operators of the ore thermal furnace (mean age: 41.9 ± 7.6 years, mean length of current employment: 3.9 ± 4.4 years). All subjects underwent a neurocognitive examination (higher brain function testing), electroneuromyography, the somatosensory evoked response test, and electroencephalography. The results of examining the higher brain function enabled us to form the neurocognitive profile of the workers. We revealed signs of mild cognitive impairment in 30 % and a decrease in the cognitive reserve in 35 % of the cases. The diagnosed peripheral nervous system disorders included distal sensory polyneuropathy of the upper and lower extremities, carpal and cubital tunnel syndromes, cervical and lumbar radiculopathy. The comprehensive neurophysiological examination helps detect early changes in the central and peripheral nervous systems. The findings should be taken into account when developing personal medical rehabilitation programs.
Statistics as an Instrument of Ideological Confrontation
Authors: Davletshina L. A. , Karmanov M. V.
Published: October 2023

The relevance of the study lies in the fact that only with a competent comprehensive assessment of quantitative parameters, their qualitative interpretation is possible. Statistics acts as such a tool.

The purpose of the study. The purpose of this paper is to study opinions and approaches in the use of statistics as a tool for assessing phenomena and processes.

Materials and methods. In the course of the study, the main directions of statistics as a tool for working with data were studied. The most common and practically justified is the use of statistics as a tool of cognition, management, propaganda and control. The result of the interpretation of statistical data primarily depends on the degree of objectivity and the level of literacy of those who work with the data: both at the initial stage of the formation of the initial data set, and at the stage of explaining the resulting values.

Results. It is revealed that the same parameters (proportions) can be interpreted in completely different ways. Such differences are formed both from the subjectivity of the perception of these data, and from the degree of study of the material. As a result, there is a field for discrepancies and discussion.

Conclusion. In order to obtain a qualitative analysis and interpretation of phenomena and processes, serious knowledge is needed, backed up by a substantial study of the subject area – only in this case statistical data can become a “mirror” reflecting the realities.
BRD4 Protein as a Target for Lung Cancer and Hematological Cancer
Therapy: A Review
Authors: Zhang Mengmeng , Li Yingbo , Zhang Zilong , Zhang Xin , Wang Wei , Song Xiaomei , Zhang Dongdong
Published: October 2023

The BET protein family plays a crucial role in regulating the epigenetic landscape of
the genome. Their role in regulating tumor-related gene expression and its impact on the survival
of tumor cells is widely acknowledged. Among the BET family constituents, BRD4 is a significant
protein. It is a bromodomain-containing protein located at the outer terminal that recognizes
histones that have undergone acetylation. It is present in the promoter or enhancer region of the target
gene and is responsible for initiating and sustaining the expression of genes associated with tumorigenesis.
BRD4 expression is significantly elevated in various tumor types. Research has indicated
that BRD4 plays a significant role in regulating various transcription factors and chromatin
modification, as well as in repairing DNA damage and preserving telomere function, ultimately
contributing to the survival of cancerous cells. The protein BRD4 has a significant impact on antitumor
therapy, particularly in the management of lung cancer and hematological malignancies,
and the promising potential of BRD4 inhibitors in the realm of cancer prevention and treatment is
a topic of great interest. Therefore, BRD4 is considered a promising candidate for prophylaxis and
therapy of neoplastic diseases. However, further research is required to fully comprehend the significance
and indispensability of BRD4 in cancer and its potential as a therapeutic target.
Multifaceted Understandings of Cancer: A Review of Disease Mechanisms and Therapies
Authors: Pal Rashmi Saxena , Pal Yogendra , Chaitanya Motamarri Venkata Naga Lalitha , Babu Ravindra , Mazumder Rupa , Kumar Arvind
Published: October 2023

Cancer is a condition in which aberrant cells continue to divide uncontrollably, causing
tissue to be destroyed. Different cancer stages have been identified by researchers, suggesting that a
number of gene alterations are involved in the etiology of cancer. Unusual cell proliferation is
brought on by these gene alterations. A crucial role in the acceleration of cell proliferation is played
by genetic diseases brought on by heredity or hereditary factors. Its diagnosis and treatment have historically
been regarded as two of the most important and significant clinical concerns. Depending on
the nature and stage of the tumor, numerous ways to its corrective therapy have been established.
Numerous biomolecules derived from plants are crucial for the treatment of the disease. In this review,
we focus on specific aspects to give a brief overview of the major problems, including their
etiology, therapies that are available, herbal alternates, available vaccines and relevant biomarkers. It
was felt that much work needs to be done on combination therapy development and platform optimization.
Does financial digitalization affect macroeconomic stability in Indonesia? An application of the autoregressive distributed lag (ARDL) model
Authors: Sudrajad Oktofa Yudha , Wiryono Sudarso Kaderi , Kitri Mandra Lazuardi , Rahadi Raden Aswin , Saputra Jumadil , Adhinugroho Triasto
Published: October 2023

Digitalization has transformed the monetary system more radical for many years. This study aims to investigate the effect of digital payments on macroeconomic stability. Electronic money is used as a proxy for digital payment. The macroeconomic stability is calibrated using exchange rate volatility and inflation rate. This study uses monthly data ranging from January 2009 to March 2020. Macroeconomic data were collected from the Indonesian Central Bureau of Statistics and the Organisation for Economic Co-operation and Development. Industry and market data from the Central Bank of Indonesia (Statistic of Bank Indonesia) and the Indonesian Stock Exchange (IDX). The data were analyzed using the Autoregressive Distributed Lag (ARDL) to examine the long-run and short-run relationship between the studied variables. This study found that digital payments affect Indonesian macroeconomic stability. Electronic money as a proxy of digitalization has a positive and significant relationship with exchange rate volatility and inflation. Cross-border e-commerce might induce exchange rate volatility due to its convenience as a one-stop shopping service and its lower switching cost of currency. The driver of higher inflation is electronic money, which increases people's spending, thus increasing the velocity of circulation and total consumption.
Trial of the Pluslife SARS-CoV-2 Nucleic Acid Rapid Test Kit: Prospective Cohort Study
Authors: Zhu Dandan , Huang Jing , Hu Bei , Cao Donglin , Chen Dingqiang , Song Xinqiang , Chen Jialing , Zhou Hao , Cen Aiqun , Hou Tieying
Published: October 2023

Background

In response to the SARS-CoV-2 epidemic, a convenient, rapid, and sensitive diagnostic method for detecting COVID-19 is crucial for patient control and timely treatment.

Objective

This study aimed to validate the detection of SARS-CoV-2 with the Pluslife SARS-CoV-2 rapid test kit developed based on a novel thermostatic amplification technique called RNase hybridization-assisted amplification.

Methods

From November 25 to December 8, 2022, patients with suspected or confirmed COVID-19, close contacts, and health care workers at high risk of exposure were recruited from 3 hospitals and 1 university. Respiratory specimens were collected for testing with the Pluslife SARS-CoV-2 rapid test kit and compared with reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and a commercial antigen assay kit. Samples from 1447 cases were obtained from 3 “ready-to-test” scenarios in which samples were collected on site and tested immediately, and samples from 503 cases were obtained from a “freeze-thaw test” scenario in which samples were collected, frozen, and thawed for testing.

Results

Pluslife SARS-CoV-2 rapid testing of samples from the “ready-to-test” scenario was found to be accurate (overall sensitivity and specificity of 98.3% and 99.3%, respectively) and diagnostically useful (positive and negative likelihood ratios of 145.45 and 0.02, respectively). Pluslife SARS-CoV-2 rapid testing of samples from the “freeze-thaw test” scenario was also found to be accurate (overall sensitivity and specificity of 71.2% and 98.6%, respectively) and diagnostically useful (positive and negative likelihood ratios of 51.01 and 0.67, respectively). Our findings demonstrated that the time efficiency and accuracy of the results in a “ready-to-test” scenario were better. The time required from sample preparation to the seeing the result of the Pluslife SARS-CoV-2 rapid test was 10 to 38 minutes, which was substantially shorter than that of RT-qPCR (at least 90 minutes). In addition, the diagnostic efficacy of the Pluslife SARS-CoV-2 rapid test was better than that of a commercial antigen assay kit.

Conclusions

The developed RNase hybridization-assisted amplification assay provided rapid, sensitive, and convenient detection of SARS-CoV-2 infection and may be useful for enhanced detection of COVID-19 in homes, high-risk industries, and hospitals.
O IMPACTO DO TRANSTORNO OBSESSIVO-COMPULSIVO NAS RELAÇÕES FAMILIARES: uma replicação sistemática
Authors: Gonçalves Lariane , Ferreira André Luíz
Published: October 2023

Obsessive-compulsive Disorder (OCD) is characterized by the presence of obsessions and/or compulsions that arise in a way that consumes excessive time for affected individuals, causing functional impairment and significant suffering. Considering that the family has played the role of main caregiver in the current psychosocial project, the present study aimed to investigate the overload of family members of patients previously with OCD, aiming to be a systematic replication of the study by Soares Neto et al. (2011), entitled “Overburden in family members of individuals with obsessive-compulsive disorder”. For data collection the Family Burden Assessment Scale (FBIS-BR) and a questionnaire that collected sociodemographic data and related to family life were used. Seven family members of subjects with OCD participated in this research, selected by degree of proximity, over 18 years old and without ongoing mental disorders. The research carried out a survey study, with quantitative and qualitative data analysis. Its purpose was to analyze and understand the degree of objective and subjective overload of these family members. As main results high levels of overload were found, especially in the items covered by the subjective dimension of the scale, although the overall objective score was higher. Subscale A (referring to the frequency of performing daily activities) recorded the greatest impact on objective burden; while subscale E (referring to concern for psychiatric patients), compared to all subscales, had the highest mean score. In view of this, it is essential that there is greater support from health services and professionals to families, considering that they have occupied the position of main caregiver.
PRÁTICAS PSICOLÓGICAS NO CONTEXTO HOSPITALAR PELA ABORDAGEM CENTRADA NA PESSOA: uma revisão sistemática de literatura
Authors: Fiorotti Mayara Aparecida , Pereira Leihge Roselle Rondon , Pecora Calhao Ana Rafaela
Published: October 2023

From a personal experience added to the construction of academic knowledge, the desire to expand knowledge about the work of the hospital psychologist based on the Person-Centered Approach (PCA), in Brazil. In this sense, this research, with a qualitative approach, aims to understand the ways of acting of these professionals, as well as to analyze the discussions and notes they make during their practices. For this, an exploratory documentary research was carried out, which used as methodology the Systematic Literature Review (RSL). Data were collected in the databases SciELO, Periódicos CAPES, BVS Brasil, Revista Gestáltica, NUFEN, Redalyc. Grey Literature was also used to map other data sources, such as the electronic portal Encontro ACP and to include references indicated by researchers of this approach, who are appointed as experts. We used the qualitative descriptive analysis, as well as the theoretical framework of the PCA to understand the results. These outline the conduct of the psychologist based on the PCA in the health/hospital context, where he sees it being possible to act in different spaces within the institution, with different audiences and psychotherapeutic formats, making it possible to assist those involved in the context to ensure a humanized performance, based on the assumptions of the PCA.
FATORES QUE INFLUENCIAM NA LONGEVIDADE DE RESINAS COMPOSTAS: revisão narrativa de literatura
Authors: Oliveira Thays Valleska Rodrigues , Lopes Vitória Rafaella Pereira , Andrade Cláudia Maria de Oliveira
Published: October 2023

Currently, there is an increasing demand for procedures focused on aesthetic rehabilitation and minimally invasive interventions in dental clinical settings. These interventions coexist with restorative procedures, which already hold a notable presence in daily clinical routines. Contrary to the widely held assumption, the success of a restoration is not solely determined by the selection of high-quality resin, but is also contingent upon other criteria of significant relevance. These encompass pre-existing clinical conditions, the operator's skill, the patient's profile, and the materials employed. In this vein, this study aims to present the key factors influencing the longevity of composite resin restorations, as well as to provide guidance to the dentist regarding the importance of correctly adhering to the clinical sequence during the restorative protocol, and ultimately, to elucidate the influence of external factors: the patient's acidic diet, gastric reflux, poor oral hygiene, alcohol consumption, and smoking. To this end, qualitative research was conducted through narrative literature review. Furthermore, regular polishing of the restoration during maintenance visits is essential to preserve its properties and enhance its durability.
Nuevos antecedentes de parásitos gastrointestinales presentes en razas ovinas de carne en Magallanes
Authors:   Instituto de Investigaciones Agropecuarias. INIA, Sales Francisco , Sandoval Camila , Muñoz Pamela ,   Instituto de Investigaciones Agropecuarias. INIA,   FACULTAD DE CS. VETERINARIAS, UNIVERSIDAD AUSTRAL DE CHILE, CAMPUS ISLA TEJA S/N° VALDIVIA
Published: October 2023

Con el objeto de describir la situación parasitaria gastrointestinal de las razas carniceras ovinas introducidas a Magallanes y su efecto sobre su crecimiento, corderos de las razas Polled Dorset, Texel y Suffolk fueron seleccionados post destete. Se controlaron 35 machos y 35 hembras por raza. A los 3 meses fueron destetados y se obtuvo tanto los pesos mensuales, así como muestras de fecas para recuento y cultivo. A los 3 meses de edad, no se evidencia diferencia entre las razas para el recuento total de parásitos. Se observó una interacción raza*sexo*parto para estróngilos (P=0,0056), donde machos mellizos de la raza Polled Dorset mostraron una carga parasitaria superior a machos únicos, hembras mellizas de la misma raza y de la Suffolk, machos y hembras únicos Suffolk y Texel. A los 6 meses de edad, la totalidad de animales se encuentra parasitado y se observó una interacción raza*sexo*parto (P=0,044) para Nematodirus, donde hembras de parto único, de la raza Suffolk, presentaron menores recuentos que machos mellizos de la misma raza y de la raza Texel. No se observó diferencia al cultivo larvario entre las razas de ovinos evaluadas. Existió una correlación negativa entre los pesos a los 5 meses (r2=-0,28; P=0,025), 6 (r2=-0,32; P=0,01) y 7 (r2=-0,37; P=0,004), con el recuento de Nematodirus a los 6 meses. En conclusión, existiría parasitosis en animales de razas carniceras, si bien estos recuentos son bajos, pero la raza Suffolk aparece más sensible que Texel y Polled Dorset, lo que debe ser considerado en los manejos sanitarios.
Konsep Uslub Amr Bagi Konteks Akidah dalam Surah Al-Mulk Berdasarkan Pendapat Al-Awsi
Authors: @ Mohd Rifain Syakirah Rifa’in , Pa Mat Taib , Hussin Mohamad , Latif Faizuri Abd.
Published: October 2023

Al-Qur’an has its own beauty secret with various uses of uslub amr. This study aims to identify the implicit or secret meaning of the use of fi‘il amr in the field related to Islamic Creed. This study is a qualitative study that uses al-Awsi’s opinion and text analysis techniques based on the context of the verse by using related interpretations from past and modern scholars. Overall it is found that the use of amr for the context of Islamic Creed in surah al-Mulk is not used in any other form except fi‘il amr only. The study found that there are ten verses that use fi‘il amr for the context of Islamic Creed in surah al-Mulk. The study summarizes that fi‘il amr is used as a very firm and strong instruction on basic matters in the context of faith for matters of al-Ilahiyyat and al-Sam’iyyat because it involves faith in Allah, Angels, the Qur’an, the Messenger and the Day of Judgment which is obligatory believed by Muslims. The uslub amr used in the Qur’an has its own secrets and techniques because it has an implicit meaning for each form of amr. Therefore, the use of amr is planned and not without a specific arrangement. This needs to be learned so that it can be used as a guide and lesson in everyday life.
Developing Islamic Psychospiritual Guidelines for Covid-19 Task Counsellors
Authors: S.M.H.S.A. Rahman , Mohd Azhar Muhammad Hazim , Ramli Mohd Anuar , Ahmad Sabki Zuraida , Nasiruddin Nabil
Published: October 2023

The Covid-19 pandemic has left significant implications on the emotional and psychological health as well as their personal well-being. As an effort in managing the problem, many individuals undergo counselling sessions with counsellors in the hope of gaining emotional and psychological support. However, the continuous dumping of client narratives during the Covid-19 pandemic has led to repercussions on emotional stress such as Secondary Traumatic Stress (STS) problems for these counsellors. By considering the solution to this problem from the Islamic perspective that states that human beings are also made up of the spiritual aspect, this study is aimed at developing an Islamic psychospiritual guideline for counsellors on duty during the Covid-19 pandemic. The study is conducted in three phases; the Needs Analysis Phase, Guideline Development Phase and Evaluation Phase. In the Needs Analysis Phase, literature review and interviews are employed to identify the items needed in the development of the guidelines. The guideline development phase is the process of completing the draft guidelines using the psychospiritual counselling theory as the foundation. This theory is an approach that combines the spiritual and religious aspects derived from the Qur’an and Hadith. Next is the evaluation phase where the guidelines developed are evaluated and validated by experts in the field of Islamic counselling, psychology and psychospiritual. This study presents the Islamic psychospiritual guidelines as preparation for counsellors in facing clients in an effort to avoid psychological disorders. The guidelines consist of two classifications namely; 1) mental and emotional preparation as a daily practice, before, during and after a session; and 2) spiritual preparation as a daily practice, before, during and after a session. It is hoped that these guidelines can be applied in the practice among Muslim counsellors as a holistic preparation that embraces the psychological and spiritual balance based on Islamic teachings.
Outcomes of children with newly diagnosed clear cell sarcoma of kidney following NWTS strategies in Shanghai China (2003–2021)
Authors: Zhang Anan , Yuan Xiaojun , Jiang Shayi , Xu Dongqing , Huang Can , Tang Jingyan , Gao Yijin
Published: October 2023

Background: Although clear cell sarcoma of kidney (CCSK) is
rare, it is the second most common renal tumor in children after Wilms’
tumor. Here we aimed to evaluate the characteristics and outcomes of
CCSK in China. Methods: For this multicenter retrospective
study, a total of 54 patients were enrolled from three children’s
hospitals, between April 2003 and December 2021. Treatment comprised
upfront radical nephrectomy, followed by radiotherapy and intensive
chemotherapy. Clinical records were regularly updated. Prognostic
factors and survival rates were evaluated. Results: The 54
enrolled patients had a median age of 37 months (range, 4 months to 11.4
years). The stage distribution was 16% stage I (n=9), 30% stage II
(n=16), 39% stage III (n=21), and 15% stage IV (n=8). After a median
follow-up of 5.6 years, the 5-year event-free survival (EFS) was
82.4±5.4%, and overall survival was 88.1±4.6%. The EFS was 100% for
stage I, 93.8 ±6.1% for stage II, 71.1±10.0% for stage III, and
68.6±18.6% for stage IV. Univariate analysis revealed that staging,
tumor rupture, and inferior vena cava tumor thrombus were inferior
prognostic factors. Among relapsed patients, relapse occurred a median
of 11 months after diagnosis (range, 4–41 months), and 50% (4/8)
achieved a second complete remission. Conclusions: The outcomes
of patients with stage I–III CCSK in China were comparable to findings
in other developed countries. Better outcomes were achieved in stage IV
CCSK by using an intensive chemotherapy regimen including
carboplatin，which require confirmation by AREN0321. Treatment should be
encouraged even in CCSK cases with metastasis and relapse. This clinical
trial is registered with ChiCTR-PRCH-14004372.
Fatty Acids Increase GDF15 and Reduce Food Intake Through a GFRAL Signaling Axis
Authors: Wang Dongdong , Joy Therese Jabile Maria , Lu Junfeng , K. Townsend Logan , M. Valvano Celina , Gautam Jaya , Batchuluun Battsetseg , E. Tsakiridis Evangelia , S.V. Lally James , R. Steinberg Gregory
Published: October 2023

Abstract: In contrast to the well-defined biological feedback loops controlling glucose, the mechanisms by which the body responds to changes in fatty acid availability are less clearly defined. GDF15 suppresses the consumption of diets high in fat but is paradoxically increased in obese mice fed a high-fat diet. Given this interrelationship, we investigated whether diets high in fat could directly increase GDF15 independently of obesity. We found that fatty acids increase GDF15 levels dose-dependently with the greatest response observed with linolenic acid. GDF15 mRNA expression was modestly increased in the gastrointestinal tract, however, kidney GDF15 mRNA was ~1000-fold higher and was increased by over 3-fold with subsequent RNAscope analysis showing elevated expression within the cortex and outer medulla. Treatment of wildtype mice with linolenic acid reduced food intake and body mass, however, this effect was disappeared in mice lacking the GDF15 receptor GFRAL. An equal caloric load of glucose did not suppress food intake or reduce body mass in either WT or GFRAL KO mice. These data indicate that fatty acids such as linolenic acid increases GDF15 and suppresses food intake through a mechanism requiring GFRAL. These data suggest that a primary physiological function of GDF15 may be as a fatty acid sensor designed to protect cells from fatty acid overload.

Article Highlights:

· The mechanisms by which the body responds to changes in fatty acid availability are less clearly defined.

· We investigated whether diets high in fat could directly increase GDF15 independently of obesity.

· Fatty acids increase GDF15 and reduce food intake through a GFRAL signaling axis.

· GDF15 is a sensor of fatty acids that may have important implications for explaining increased satiety following consumption of diets high in fat.
Pemanfaatan Kelapa dan Pemberdayaan Potensi Desa sebagai Desa Wisata Berbasis Kearifan Lokal
Authors: Anindyajati Adhi
Published: October 2023

Giripeni Village is one of the villages in Wates District, Kulon Progo Regency. Giripeni village is on the outskirts of the city, the area is mostly rice fields. Most of the people work as farmers and breeders. The availability of abundant foodstuffs such as grass and secondary crops is used to raise livestock. Giripeni Village has a lot of potential that can be developed, so that it becomes a tourist village to attract the wider community, both nationwide and worldwide from Giripeni Village. The development of this tourism village is one of the rural tourism developments which is expected to become a sustainable tourism development in accordance with government policies in the tourism sector. On the other hand, during the Covid-19 epidemic, many workers lost their jobs and some required workers to do their work from home. Group 88 provides video on using coconut fruit, so that people in Giripeni village whose jobs are affected by the Covid-19 pandemic can remain productive even at home. The method provided to process the coconut involves a simple steps and efficient use of materials. The research was carried out through online observation. The data in this study are secondary data obtained from the Giripeni village website and secondary data obtained based on previous research