bluuebunny/crossref_metadata_2025 huggingface dataset:
Nurturing Family Environments for Children: Compassion-Focused Parenting as a Form of Parenting Intervention
Authors: Kirby James N.
Published: December 2019

The style of parenting a child receives has profound long-term impacts on that child’s life. Yet, the rates of child maltreatment globally are high (in both developing and developed countries), indicating that many children around the world are being raised in toxic environments. Evidence-based parenting programs (EBPPs) have been demonstrated to have positive impacts on improving parenting style, whilst reducing childhood social, emotional and behavioural problems. EBPPs originated out of a need to address externalizing behaviour problems and to address conduct problems, and compliance became a key target of these parenting models. Thus, many EBPPs were developed in an era where operant and social learning theory-based approaches to parenting were most prominent and these parenting models still prevail today. This paper has one major aim—to demonstrate how the next generation of EBPPs need to be grounded in evolved caring motivational systems and affiliative emotion processing, which requires an understanding of the evolved processes involved in parent-offspring caring. This new approach to parenting is called, ‘compassion-focused parenting’, and this new approach to parenting will be described.
Research on Model Predictive Control of IPMSM Based on Adaline Neural Network Parameter Identification
Authors: Wang Lihui , Tan Guojun , Meng Jie
Published: December 2019

This paper reports the optimal control problem on the interior permanent magnet synchronous motor (IPMSM) systems. The control performance of the traditional model predictive control (MPC) controller is ruined due to the parameter uncertainty and mismatching. In order to solve the problem that the MPC algorithm has a large dependence on system parameters, a method which integrates MPC control method and parameter identification for IPMSM is proposed. In this method, the d-q axis inductances and rotor permanent magnet flux of IPMSM motor are identified by the Adaline neural network algorithm, and then, the identification results are applied to the predictive controller and maximum torque per ampere (MTPA) module. The experimental results show that the optimized MPC control proposed in this paper has a good steady state and robust performance.
Analysis of Local Measurement-Based Algorithms for Fault Detection in a Multi-Terminal HVDC Grid
Authors: Pérez Molina María José , Larruskain Dunixe Marene , Eguía López Pablo , Etxegarai Agurtzane
Published: December 2019

One of the most important challenges of developing multi-terminal (MT) high voltage direct current (HVDC) grids is the system performance under fault conditions. It must be highlighted that the operating time of the protection system needs to be shorter than a few milliseconds. Due to this restrictive requirement of speed, local measurement based algorithms are mostly used as primary protection since they present an appropriate operation speed. This paper focuses on the analysis of local measurement based algorithms, specifically overcurrent, undervoltage, rate-of-change-of-current, and rate-of-change-of-voltage algorithms. A review of these fault detection algorithms is presented. Furthermore, these algorithms are applied to a multi-terminal grid, where the influence of fault location and fault resistance is assessed. Then, their performances are compared in terms of detection speed and maximum current interrupted by the HVDC circuit breakers. This analysis aims to enhance the protection systems by facilitating the selection of the most suitable algorithm for primary or backup protection systems. In addition, two new fault type identification algorithms based on the rate-of-change-of-voltage and rate-of-change-of-current are proposed and analyzed. The paper finally includes a comparison between the previously reviewed local measurement based algorithms found in the literature and the simulation results of the present work.
Prediction of the Energy Consumption Variation Trend in South Africa based on ARIMA, NGM and NGM-ARIMA Models
Authors: Ma Minglu , Wang Zhuangzhuang
Published: December 2019

South Africa’s energy consumption takes up about one-third of that in the whole African continent, ranking the first place in Africa. However, there are few researches on the prediction of energy consumption in South Africa. In this study, based on the data of South Africa’s energy consumption during 1998–2016, Autoregressive Integrated Moving Average (ARIMA) model, nonlinear grey model (NGM) and nonlinear grey model–autoregressive integrated moving average (NGM-ARIMA) model are adopted to predict South Africa’s energy consumption during 2017–2030. After using these NGM, ARIMA and NGM-ARIMA, the mean absolute percent errors (MAPE) are 2.827%, 2.655% and 1.772%, respectively, which indicates that the predicted result has very high reliability. The prediction results show that the energy consumption in South Africa will keep increasing with the growth rate of about 7.49% in the next 14 years. This research result will provide scientific basis for the policy adjustment of energy supply and demand in South Africa and the prediction techniques used in the research will have reference function for the energy consumption study in other African countries.
Causes of Delay in Power Transmission Projects: An Empirical Study
Authors: Pall Goutom K. , Bridge Adrian J. , Gray Jason , Skitmore Martin
Published: December 2019

Power transmission (PT) projects are vital for the power sector. However, worldwide PT projects experience delay. There is an urgent need to understand the unique causes of delays in PT projects. This paper presents the first empirical study on causes of delays in PT projects via a comprehensive literature review. Based on this literature review, 63 potential delay factors are identified and divided into ten major groups. These include two new groups of delay attributes, comprising sector-specific factors (SSF) and general factors (GF), where SSF pertains solely to PT projects and GF contributes to minimizing the bias of project participants. A questionnaire survey of 311 PT stakeholders is conducted to determine the overall ranking of the delay factors using the relative importance index. The results indicate that SSF, GF, and external/unavoidable factors are the most critical groups of delay factors, with the top-ranked factors being right of way problems of transmission line (TL), frequent changes in TL routes, and accessibility to the TL tower locations. Finally, recommendations are made to help minimize time overruns in PT projects, as well as in other linear power and non-power construction projects in general.
Occupational Exposure on Board Fishing Vessels: Risk Assessments of Biomechanical Overload, Noise and Vibrations among Worker on Fishing Vessels in Southern Italy
Authors: Mansi Francesca , Cannone Enza Sabrina Silvana , Caputi Antonio , De Maria Luigi , Lella Leonardo , Cavone Domenica , Vimercati Luigi
Published: December 2019

Sea fishing is one of the sectors with the highest risk of illness and work-related accidents. The purpose of the study is to evaluate the exposure of fishing workers to three major risks: biomechanical overload, noise, and whole-body vibrations. We used common methods and measurement tools in the field: observational video analysis, questionnaires, and direct measurement. Noise and vibrations levels were measured aboard five boats belonging to the main fishing communities of Southern Italy. The random sample consisted of 310 workers, of whom 108 agreed to complete a questionnaire to collect data on the perception of occupational risk and self-perception of health conditions. We found that fishermen had a high prevalence of osteoarticular pathologies (42%) and that the biomechanical overload risk is mainly related to handling manual loads. Furthermore, the results indicate that the levels of weekly noise exposure exceed the exposure limit value of 87 decibel A (dBA) for fishing workers, and that the most noisiest area is the engine room. Exposure levels to whole-body vibrations were below <0.5 m/s2. Knowledge on occupational hazards and health effects in the fisheries sector should be used to develop ship technology, raise awareness of the correct use of personal protective equipment, and improve health surveillance of these workers.
Visualization and Localization of Submicron-Sized Ammonium Sulfate Particles on Needles of Japanese Larch (Larix kaempferi) and Japanese Cedar (Cryptomeria japonica) and Leaves of Japanese Beech (Fagus crenata) and Japanese Chinquapin (Castanopsis sieboldii) after Artificial Exposure
Authors: Yamane Kenichi , Nakaba Satoshi , Yamaguchi Masahiro , Kuroda Katsushi , Sano Yuzou , Lenggoro I. Wuled , Izuta Takeshi , Funada Ryo
Published: December 2019

We applied a method combining field-emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectrometry (EDX) to visualize the deposition and localization of the submicron-sized ammonium sulfate (AS) particles. The AS particles emitted from an aerosol generator in the laboratory were spherical in shape and individually deposited without aggregation on the surface of a silicon substrate. We determined the AS particles on the surfaces of the needles of Japanese larch (Larix kaempferi) and Japanese cedar (Cryptomeria japonica), and the leaves of Japanese beech (Fagus crenata) and Japanese chinquapin (Castanopsis sieboldii), using EDX. The particles were deposited on either the adaxial or abaxial side of the leaves and needles. The AS particles deposited on the surfaces of the leaves and needles did not aggregate, and they were deposited on the surfaces of the leaves and needles in the same manner, regardless of leaf structure. These results, using a new method, highlight the early stages of the deposition and localization of submicron-sized AS particles on the surfaces of the leaves and needles of forest trees.
Biological Durability of Sapling-Wood Products Used for Gardening and Outdoor Decoration
Authors: Brischke Christian , Emmerich Lukas , Nienaber Dirk G.B. , Bollmus Susanne
Published: December 2019

Sapling-wood products from different wood species such as willow (Salix spp. L.) and Common hazel (Corylus avellana L.) are frequently used for gardening and outdoor decoration purposes. Remaining bark is suggested to provide additional biological durability. Even for temporary outdoor use it seemed questionable that durability of juvenile sapwood can provide acceptably long service lives of horticultural products. Therefore, sapling-wood from seven European-grown wood species was submitted to laboratory and field durability tests. In field tests, specimens with and without bark were tested in comparison and submitted to differently severe exposure situations, i.e., in-ground contact, and above-ground situations with and without water trapping. All materials under test were classified ‘not durable’ independently from any potential protective effect of remaining bark, which contradicted their suitability for outdoor applications if multi-annual use is desired.
Accumulation and Translocation of Phosphorus, Calcium, Magnesium, and Aluminum in Pinus massoniana Lamb. Seedlings Inoculated with Laccaria bicolor Growing in an Acidic Yellow Soil
Authors: Gu Xirong , Wang Xiaohe , Li Jie , He Xinhua
Published: December 2019

Research Highlights: We demonstrate that ectomycorrhizal (ECM) fungi improve plant aluminum (Al)-tolerance in the field and Laccaria bicolor S238A is a promising ECM isolate. Furthermore, we interpret the underlying nutritional mechanism that ECM inoculation facilitates aboveground biomass production as well as nutrients accumulation and translocation. Background and Objectives: Al toxicity is a primary limiting factor for plants growing in acidic soils. Hydroponic/sand culture studies have shown that some ECM fungi could enhance plant Al-tolerance. However, the underlying mechanisms of ECM fungi in improving plant Al-tolerance in the field are still unknown. To fill this knowledge gap, the present study aimed to examine roles of ECM inoculation in biomass production, accumulation and translocation of nutrients and Al in the host plant grown in the field under Al treatment. Materials and Methods: 4-week-old Pinus massoniana seedlings were inoculated with three Laccaria bicolor isolates (L. bicolor 270, L. bicolor S238A or L. bicolor S238N) and grown in an acidic yellow soil under 1.0 mM Al treatment for 12 weeks in the field. Biomass production, accumulation and translocation of P, Ca, Mg, and Al were investigated in these 16-week-old P. massoniana seedlings. Results: All three of these L. bicolor isolates improved biomass production as well as P, Ca and Mg accumulation in P. massoniana seedlings. Moreover, the three ECM isolates facilitated the translocation of P, Ca, and Mg to aboveground in response to Al treatment, particularly when seedlings were inoculated with L. bicolor S238A. In addition, both L. bicolor 270 and L. bicolor S238A had no apparent effects on Al accumulation, while enhanced Al translocation to aboveground. In contrast, L. bicolor S238N decreased Al accumulation but had no significant effect on Al translocation. Conclusions: ECM fungi in the field improved plant Al-resistance by increasing nutrient uptake, and this was mostly due to translocation of P, Ca, and Mg to aboveground, not by decreasing the uptake and translocation of Al.
Lidar-Derived Tree Crown Parameters: Are They New Variables Explaining Local Birch (Betula sp.) Pollen Concentrations?
Authors: Bogawski Paweł , Grewling Łukasz , Dziób Katarzyna , Sobieraj Kacper , Dalc Marta , Dylawerska Barbara , Pupkowski Dominik , Nalej Artur , Nowak Małgorzata , Szymańska Agata , Kostecki Łukasz , Nowak Maciej M. , Jackowiak Bogdan
Published: December 2019

Birch trees are abundant in central and northern Europe and are dominant trees in broadleaved forests. Birches are pioneer trees that produce large quantities of allergenic pollen efficiently dispersed by wind. The pollen load level depends on the sizes and locations of pollen sources, which are important for pollen forecasting models; however, very limited work has been done on this topic in comparison to research on anthropogenic air pollutants. Therefore, we used highly accurate aerial laser scanning (Light Detection and Ranging—LiDAR) data to estimate the size and location of birch pollen sources in 3-dimensional space and to determine their influence on the pollen concentration in Poznań, Poland. LiDAR data were acquired in May 2012. LiDAR point clouds were clipped to birch individuals (mapped in 2012–2014 and in 2019), normalised, filtered, and individual tree crowns higher than 5 m were delineated. Then, the crown surface and volume were calculated and aggregated according to wind direction up to 2 km from the pollen trap. Consistent with LIDAR data, hourly airborne pollen measurements (performed using a Hirst-type, 7-day volumetric trap), wind speed and direction data were obtained in April 2012. We delineated 18,740 birch trees, with an average density of 14.9/0.01 km2, in the study area. The total birch crown surface in the 500–1500 m buffer from the pollen trap was significantly correlated with the pollen concentration aggregated by the wind direction (r = 0.728, p = 0.04). The individual tree crown delineation performed well (r2 ≥ 0.89), but overestimations were observed at high birch densities (> 30 trees/plot). We showed that trees outside forests substantially contribute to the total pollen pool. We suggest that including the vertical dimension and the trees outside the forest in pollen source maps have the potential to improve the quality of pollen forecasting models.
Genome-Wide Identification and Characterization of Heat-Shock Transcription Factors in Rubber Tree
Authors: Li Yan , Yu Wencai , Chen Yueyi , Yang Shuguang , Wu Shaohua , Chao Jinquan , Wang Xinlong , Tian Wei-Min
Published: December 2019

Heat-shock transcription factors (Hsfs) play a pivotal role in the response of plants to various stresses. The present study aimed to characterize the Hsf genes in the rubber tree, a primary global source of natural rubber. In this study, 30 Hsf genes were identified in the rubber tree using genome-wide analysis. They possessed a structurally conserved DNA-binding domain and an oligomerization domain. On the basis of the length of the insert region between HR-A and HR-B in the oligomerization domain, the 30 members were clustered into three classes, Classes A (18), B (10), and C (2). Members within the same class shared highly conserved gene structures and protein motifs. The background expression levels of 11 genes in cold-tolerant rubber-tree clone 93-14 were significantly higher than those in cold-sensitive rubber-tree clone Reken501, while four genes exhibited inverse expression patterns. Upon cold stress, 20 genes were significantly upregulated in 93-114. Of the upregulated genes, HbHsfA2b, HbHsfA3a, and HbHsfA7a were also significantly upregulated in three other cold-tolerant rubber-tree clones at one or more time intervals upon cold stress. Their nuclear localization was verified, and the protein–protein interaction network was predicted. This study provides a basis for dissecting Hsf function in the enhanced cold tolerance of the rubber tree.
Pine Pitch Canker (PPC): Pathways of Pathogen Spread and Preventive Measures
Authors: Zamora-Ballesteros Cristina , Diez Julio J. , Martín-García Jorge , Witzell Johanna , Solla Alejandro , Ahumada Rodrigo , Capretti Paolo , Cleary Michelle , Drenkhan Rein , Dvořák Miloň , Elvira-Recuenco Margarita , Fernández-Fernández Mercedes , Ghelardini Luisa , Gonthier Paolo , Hernández-Escribano Laura , Ioos Renaud , Markovskaja Svetlana , Martínez-Álvarez Pablo , Muñoz-Adalia E. Jordán , Nowakowska Justyna Anna , Oszako Tomasz , Raposo Rosa , Santini Alberto , Hantula Jarkko
Published: December 2019

Fusarium circinatum (Nirenberg and O’ Donnell) is the causal agent of pine pitch canker (PPC) disease, one of the most devastating forest diseases worldwide. Long-distance spread occurs mainly through the movement of infected seeds whereas at regional level, the movement of seedlings, substrates, or containers may play an important role in fungal dispersal. Invasion of nurseries takes place via infected seeds and further spread can occur by planting contaminated seedlings, especially due to the possibility of infected plants remaining symptomless. Once established, F. circinatum spreads by rain, wind, and insects. The natural spread of the pathogen is limited due to the short dispersal distances of the spores and the fairly short flight distances of disseminating insects. In this review, we summarize the currently known dispersal pathways of the pathogen, discussing both natural and human-assisted processes. With the purpose of understanding how to best intervene in the disease’s development in nurseries and forests, we outline the epidemiology of the pathogen describing the key factors influencing its spread. Preventive measures to control the spread of F. circinatum locally and globally are described with special emphasis on the challenges in implementing them.
Is Phylogeny More Useful than Functional Traits for Assessing Diversity Patterns Under Community Assembly Processes?
Authors: Xu Jinshi , Dang Han , Wang Mao , Chai Yongfu , Guo Yaoxin , Chen Yu , Zhang Chenguang , Yue Ming
Published: December 2019

Phylogenetic and functional diversities and their relationship are important for understanding community assembly, which relates to forest sustainability. Thus, both diversities have been used in ecological studies evaluating community responses to environmental changes. However, it is unclear whether these diversity measures can uncover the actual community assembly processes. Herein, we examined their utility to assess such assembly processes by analyzing similarities in phylogenetic, functional, and taxonomic α- and β-diversities along an elevational gradient. Additionally, we examined the relationships among environment, phylogeny, and functional traits within the community. Based on our results, we evaluated whether phylogenetic or functional diversity could better reveal the actual community assembly processes. We found that taxonomic, phylogenetic, and functional α-diversities were correlated with one another. Although the functional α-diversity showed a linear correlation with the elevational gradient, taxonomic and phylogenetic α-diversities showed unimodal patterns. Both phylogenetic and functional β-diversities correlated with taxonomic β-diversity, but there was no significant relationship between the former. Overall, our results evidenced that phylogenetic diversity and taxonomic diversity showed similar patterns, whereas functional diversity showed a relatively independent pattern, which may be due to limitations in the functional trait dimensions used in the present study. Although it is difficult to unravel whether the environment shapes phylogeny or functional traits within a community, phylogenetic diversity is a good proxy for assessing the assembly processes, whereas functional diversity may improve knowledge on the community by maximizing information about the functional trait dimensions.
Simulation-Based Cost Analysis of Industrial Supply of Chips from Logging Residues and Small-Diameter Trees
Authors: Fernandez-Lacruz Raul , Eriksson Anders , Bergström Dan
Published: December 2019

Research Highlights: The use of terminals can increase supply costs by 5–11% (when compared to direct supply), but terminals help secure supply during peak demand and cope with operational problems in the supply fleet in cases where direct supply chains would be unable to meet demand on time. Background and Objectives: This work analyses the supply cost of chipped logging residues and small-diameter trees, from chipping at roadside storages to delivery to the end-user. Factors considered include demand curves (based on the requirements of a theoretical combined heat and power plant or biorefinery); demand volume; and mode of supply (direct or combined via terminal). The impact of longer trucking distances from the sites, and supply integration between forest and other land (varying relocation distances) was also assessed. Materials and Methods: The operational environment and work of a theoretical chip supplier in northern Sweden were modelled and simulated in ExtendSim®. Results: The mean supply cost of chips was 9% higher on average for combined chains than for direct chains. Given a high demand, 8% of the annual demand could not be delivered on time without using a terminal. High supply integration of forest and other land reduced supply costs by 2%. Contractors’ annual workloads were evened out by direct supply to the biorefinery (which has a relatively steady demand) or supply via-terminal independently of the end-user. Keeping distinct chips from different sites (implying that trucks were not always fully loaded) instead of mixing chips from different sites until the trucks were fully loaded increased supply costs by 12%. Conclusions: Terminals increase supply costs, but can enable demand to be met on time when direct supply chains alone might fail. Integrated supply planning could reduce supply costs by increasing the utilization of residual biomass from other land.
Tree Height Increment Models for National Forest Inventory Data in the Pacific Northwest, USA
Authors: Woo Hyeyoung , Eskelson Bianca N. I. , Monleon Vicente J.
Published: December 2019

The United States national inventory program measures a subset of tree heights in each plot in the Pacific Northwest. Unmeasured tree heights are predicted by adding the difference between modeled tree heights at two measurements to the height observed at the first measurement. This study compared different approaches for directly modeling 10-year height increment of red alder (RA) and ponderosa pine (PP) in Washington and Oregon using national inventory data from 2001–2015. In addition to the current approach, five models were implemented: nonlinear exponential, log-transformed linear, gamma, quasi-Poisson, and zero-inflated Poisson models using both tree-level (e.g., height, diameter at breast height, and compacted crown ratio) and plot-level (e.g., basal area, elevation, and slope) measurements as predictor variables. To account for negative height increment observations in the modeling process, a constant was added to shift all response values to greater than zero (log-transformed linear and gamma models), the negative increment was set to zero (quasi-Poisson and zero-inflated Poisson models), or a nonlinear model, which allows negative observations, was used. Random plot effects were included to account for the hierarchical data structure of the inventory data. Predictive model performance was examined through cross-validation. Among the implemented models, the gamma model performed best for both species, showing the smallest root mean square error (RSME) of 2.61 and 1.33 m for RA and PP, respectively (current method: RA—3.33 m, PP—1.40 m). Among the models that did not add the constant to the response, the quasi-Poisson model exhibited the smallest RMSE of 2.74 and 1.38 m for RA and PP, respectively. Our study showed that the prediction of tree height increment in Oregon and Washington can be improved by accounting for the negative and zero height increment values that are present in inventory data, and by including random plot effects in the models.
Modelling Post-Disturbance Successional Dynamics of the Canadian Boreal Mixedwoods
Authors: Maleki Kobra , Gueye Mohamadou Alpha , Lafleur Benoit , Leduc Alain , Bergeron Yves
Published: December 2019

Natural disturbances, such as fire and insect outbreaks, play important roles in natural forest dynamics, which are characterized over long time scales by changes in stand composition and structure. Individual-based forest simulators could help explain and predict the response of forest ecosystems to different disturbances, silvicultural treatments, or environmental stressors. This study evaluated the ability of the SORTIE-ND simulator to reproduce post-disturbance dynamics of the boreal mixedwoods of eastern Canada. In 1991 and 2009, we sampled all trees (including seedlings and saplings) in 431 (256 m2) plots located in the Lake Duparquet Research and Teaching Forest (western Quebec). These plots were distributed in stands originating from seven wildfires that occurred between 1760 and 1944, and which represented a chronosequence of post-disturbance stand development. We used the 1991 inventory data to parameterize the model, and simulated short- to long-term natural dynamics of post-fire stands in both the absence and presence of a spruce budworm outbreak. We compared short-term simulated stand composition and structure with those observed in 2009 using a chronosequence approach. The model successfully generated the composition and structure of empirical observations. In long-term simulations, species dominance of old-growth forests was not accurately estimated, due to possible differences in stand compositions following wildfires and to differences in stand disturbance histories. Mid- to long-term simulations showed that the secondary disturbance incurred by spruce budworm did not cause substantial changes in early successional stages while setting back the successional dynamics of middle-aged stands and accelerating the dominance of white cedar in late-successional post-fire stands. We conclude that constructing a model with appropriate information regarding stand composition and disturbance history considerably increases the strength and accuracy of the model to reproduce the natural dynamics of post-disturbance boreal mixedwoods.
Transcriptomic Identification of Floral Transition and Development-Associated Genes in Styrax japonicus
Authors: Li Wei , Xu Zhengzhao , Zhang Cuiping , Jiang Xinqiang , Wang Kuiling
Published: December 2019

Styrax japonicus (S. japonicus) is an important flowering tree species in temperate regions, and it is regarded as a nectariferous plant. However, there have been few studies to date analyzing floral development in this species. In order to understand gene expression dynamics during S. japonicus flower development, we; therefore, prepared cDNA libraries from three distinct stages of S. japonicus. Illumina sequencing generated 31,471 differentially expressed unigenes during flower development. We additionally conducted pathway enrichment analyses using the GO and KEGG database in order to assess the functions of genes differentially expressed during different stages of the floral development process, revealing these genes to be associated with pathways including phytohormone signaling, Transcription factor, protein kinase, and circadian rhythms. In total, 4828 TF genes, 8402 protein kinase genes, and 78 DEGs related to hormone pathways were identified in flower development stages. Six genes were selected for confirmation of expression levels using quantitative real-time PCR. The gene expression data presented herein represent the most comprehensive dataset available regarding the flowering of S. japonicus, thus offering a reference for future studies of the flowering of this and other Styracaceae species.
Modeling of Aboveground Biomass with Landsat 8 OLI and Machine Learning in Temperate Forests
Authors: López-Serrano Pablito M. , Cárdenas Domínguez José Luis , Corral-Rivas José Javier , Jiménez Enrique , López-Sánchez Carlos A. , Vega-Nieva Daniel José
Published: December 2019

An accurate estimation of forests’ aboveground biomass (AGB) is required because of its relevance to the carbon cycle, and because of its economic and ecological importance. The selection of appropriate variables from satellite information and physical variables is important for precise AGB prediction mapping. Because of the complex relationships for AGB prediction, non-parametric machine-learning techniques represent potentially useful techniques for AGB estimation, but their use and comparison in forest remote-sensing applications is still relatively limited. The objective of the present study was to evaluate the performance of automatic learning techniques, support vector regression (SVR) and random forest (RF), to predict the observed AGB (from 318 permanent sampling plots) from the Landsat 8 Landsat 8 Operational Land Imager (OLI) sensor, spectral indexes, texture indexes and physical variables the Sierra Madre Occidental in Mexico. The result showed that the best SVR model explained 80% of the total variance (root mean square error (RMSE) = 8.20 Mg ha−1). The variables that best predicted AGB, in order of importance, were the bands that belong to the region of red and near and middle infrared, and the average temperature. The results show that the SVR technique has a good potential for the estimation of the AGB and that the selection of the model hyperparameters has important implications for optimizing the goodness of fit.
How Do Stakeholders Working on the Forest–Water Nexus Perceive Payments for Ecosystem Services?
Authors: Báliková Klára , Červená Tereza , De Meo Isabella , De Vreese Rik , Deniz Tuğba , El Mokaddem Abdelmohssin , Kayacan Bekir , Larabi Fadila , Lībiete Zane , Lyubenova Mariyana , Pezdevšek Malovrh Špela , Potočki Kristina , Pelyukh Oksana , Rugani Benedetto , Sarvasova Zuzana , Šálka Jaroslav , Stevanov Mirjana , Stojnic Srdjan , Jarský Vilém , Vuletić Dijana , Zahvoyska Lyudmyla , Paletto Alessandro
Published: December 2019

Nowadays, great emphasis is placed on the relationship between forest and water because forests are considered as substantial sources of many water ecosystem services. The aim of this paper is to analyze the stakeholder opinions towards the relationship between forests and water and the potential development of water-related payments for ecosystem services (PES) schemes. The study is developed in the context of COST Action CA15206–PESFOR-W (Forests for Water) aimed at synthesizing current knowledge about the PES schemes across Europe. The stakeholder opinions were mapped out using a structured questionnaire consisting of 20 questions divided into four thematic sections. The data were collected through an online survey. The results showed opinions of 142 stakeholders from 23 countries, mainly from Eastern Europe and the Mediterranean Basin. In order to analyze the collected data, the stakeholders were grouped in buyers, sellers, intermediaries, and knowledge providers. The survey results indicated that the most important category of water ecosystem services according to our sample of stakeholders is regulating services followed by provisioning services. Further findings pointed out the highest importance that shared values and direct changes in land management can have when designing water-related PES schemes. The role of public authorities and collective collaboration of different stakeholders, with emphasis on local and expert knowledge, are also identified as of crucial importance. The results show that stakeholder opinions can serve as a starting point when designing PES schemes.
Genome-Wide Identification of WOX Gene Family and Expression Analysis during Rejuvenational Rhizogenesis in Walnut (Juglans regia L.)
Authors: Chang Yingying , Song Xiaobo , Zhang Qixiang , Liu Hao , Bai Yongchao , Lei Xiashuo , Pei Dong
Published: December 2019

Rejuvenation is an efficient approach used in the cuttings of trees and horticultural crops, to improve their rooting ability, especially in difficult-to-root trees. WOX gene family members are involved in cell-fate transformation through balancing the maintenance and proliferation of the stem cells. However, there are no reports about the WOX gene family in Walnut (Juglans regia L.) and its relationship between rejuvenation and adventitious roots formation (ARF). Here, a genome-wide identification of JrWOX genes and their physical and chemical properties, phylogeny, and expression profiles in different organs and during rejuvenation-induced ARF is reported. The phenotype and histology characteristics of mature and rejuvenated cuttings (Mc and Rc) are also observed. In this study, 12 genes were identified and clustered into three groups based on phylogenetics, special domains, and conserved motifs. The gene structures and conserved motifs were relatively conserved, while the 12 sequences of the JrWOXs domain were diversified. Gene expression in root, stem, leaf, female flower, immature fruit, and zygotic embryo revealed that the expression levels of JrWOX4a, JrWOX4b, JrWOX5, JrWOX11, and JrWOX13 in the root were significantly higher than those of other JrWOXs, while only the expression of JrWOX11 was exclusive to the root organ. Additionally, rejuvenation treatment significantly induced almost all JrWOX genes, except JrWOX4a, JrWOX4b, and JrWOX13 (Rc 0 vs. Mc 0). During the ARF process, the transcripts of JrWOX11 and JrWOX5 were consecutively increased on a significance level; in contrast, the transcription levels of the other JrWOXs decreased or changed insignificantly. The phenotype and histology observation indicate that rejuvenation treatment made the base of the stem expand and reduced the thickness and density of sclerenchyma between the cortex and phloem. This might provide the conditions for the formation of new meristem niches. The results provided insight into the JrWOX genes’ general characteristics and their roles in rejuvenation-induced ARF.
The Effect of the Ultra-High-Pressure Homogenization of Protein Encapsulants on the Survivability of Probiotic Cultures after Spray Drying
Authors: Mis-Solval Kevin , Jiang Nan , Yuan Meilan , Joo Kay , Cavender George
Published: December 2019

Interest in probiotic foods and ingredients is increasing as consumers become more aware of their potential health benefits. The production of these products often involves the use of dry culture powders, and the techniques used to produce such powders often suffer from significant losses of viable cells during drying or require the use of expensive drying technologies with limited throughput (e.g., freeze drying). In this study, the authors examined whether culture survivability during spray drying could be increased via the treatment of two common protein encapsulants with ultra-high-pressure homogenization (UHPH). Lactobacillus plantarum NRRL B-1927 (also known as ATCC 10241), a probiotic strain, was suspended in either soy protein isolate (SPI) or whey protein isolate (WPI) which had been either treated with UHPH at 150 Mpa or left untreated as a control. The suspensions were then dried using either concurrent-flow spray drying (CCSD), mixed-flow spray drying (MFSD) or freeze drying (FD) and evaluated for cell survivability, particle size, moisture content and water activity. In all cases, UHPH resulted in equal or greater survivability among spray dried cultures, showed reductions in particle size measures and, except for one marginal case (CCFD SPI), significantly reduced the moisture content of the dried powders. The combination of these findings strongly suggests that UHPH could allow probiotic powder manufacturers to replace freeze drying with spray drying while maintaining or increasing product quality.
Using Content Analysis to Characterise the Sensory Typicity and Quality Judgements of Australian Cabernet Sauvignon Wines
Authors: Souza Gonzaga Lira , Capone Dimitra L. , Bastian Susan E.P. , Danner Lukas , Jeffery David W.
Published: December 2019

Understanding the sensory attributes that explain the typicity of Australian Cabernet Sauvignon wines is essential for increasing value and growth of Australia’s reputation as a fine wine producer. Content analysis of 2598 web-based wine reviews from well-known wine writers, including tasting notes and scores, was used to gather information about the regional profiles of Australian Cabernet Sauvignon wines and to create selection criteria for further wine studies. In addition, a wine expert panel evaluated 84 commercial Cabernet Sauvignon wines from Coonawarra, Margaret River, Yarra Valley and Bordeaux, using freely chosen descriptions and overall quality scores. Using content analysis software, a sensory lexicon of descriptor categories was built and frequencies of each category for each region were computed. Distinction between the sensory profiles of the regions was achieved by correspondence analysis (CA) using online review and expert panellist data. Wine quality scores obtained from reviews and experts were converted into Australian wine show medal categories. CA of assigned medal and descriptor frequencies revealed the sensory attributes that appeared to drive medal-winning wines. Multiple factor analysis of frequencies from the reviews and expert panellists indicated agreement about descriptors that were associated with wines of low and high quality, with greater alignment at the lower end of the wine quality assessment scale.
Evaluation of Dosidicus gigas Skin Extract as An Antioxidant and Preservative in Tuna Pâté
Authors: Chan-Higuera Jesús Enrique , Ezquerra-Brauer Josafat Marina , Lipan Leontina , Cano-Lamadrid Marina , Rizzitano Roberta , Carbonell-Barrachina Angel Antonio
Published: December 2019

A strategy for food preservation, based on a methanol–HCl squid skin extract (Dosidicus gigas) (JSSE), was evaluated at two concentrations in yellowfin tuna fish pâtés, which were stored at 4 and 8 °C for 20 day. The JSSE was characterized by determining its antioxidant and mutagenic activities. A yellowfin tuna pâté was elaborated, with and without the addition of the JSSE. An affective sensory analysis was performed to establish consumers’ preferences. During a 20-day storage period, the water activity (aw), pH, color difference (ΔE*ab), microbiological analysis, lipid oxidation and sensory quality attributes were evaluated, and the results were compared with the results of the butylated hydroxyanisole (BHA) and control treatments. The JSSE showed antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH●+) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS●) radicals and did not induce mutation, according to the Ames’ Salmonella test, nor chromosomal abnormalities, according to the onion root-tip cell assay. The consumer analysis demonstrated a higher preference for the pâté with the added JSSE in seven out of the eight evaluated attributes. During storage, the JSSE neither had an impact on aw nor pH, maintained lower ΔE*ab values, inhibited the microbial activity and lipid oxidation (unlike the control pâté), and preserved the sensory quality attributes, unlike the BHA and control treatments. This study showed that the JSSE has biologically active pigments that can act as antioxidants and antimicrobials in yellowfin tuna fish pâtés.
Exploring Mammalian Genome within Phase-Separated Nuclear Bodies: Experimental Methods and Implications for Gene Expression
Authors: Lesne Annick , Baudement Marie-Odile , Rebouissou Cosette , Forné Thierry
Published: December 2019

The importance of genome organization at the supranucleosomal scale in the control of gene expression is increasingly recognized today. In mammals, Topologically Associating Domains (TADs) and the active/inactive chromosomal compartments are two of the main nuclear structures that contribute to this organization level. However, recent works reviewed here indicate that, at specific loci, chromatin interactions with nuclear bodies could also be crucial to regulate genome functions, in particular transcription. They moreover suggest that these nuclear bodies are membrane-less organelles dynamically self-assembled and disassembled through mechanisms of phase separation. We have recently developed a novel genome-wide experimental method, High-salt Recovered Sequences sequencing (HRS-seq), which allows the identification of chromatin regions associated with large ribonucleoprotein (RNP) complexes and nuclear bodies. We argue that the physical nature of such RNP complexes and nuclear bodies appears to be central in their ability to promote efficient interactions between distant genomic regions. The development of novel experimental approaches, including our HRS-seq method, is opening new avenues to understand how self-assembly of phase-separated nuclear bodies possibly contributes to mammalian genome organization and gene expression.
A 24-Month Follow-Up Study of the Effect of Intra-Articular Injection of Autologous Microfragmented Fat Tissue on Proteoglycan Synthesis in Patients with Knee Osteoarthritis
Authors: Borić Igor , Hudetz Damir , Rod Eduard , Jeleč Željko , Vrdoljak Trpimir , Skelin Andrea , Polašek Ozren , Plečko Mihovil , Trbojević-Akmačić Irena , Lauc Gordan , Primorac Dragan
Published: December 2019

Osteoarthritis (OA) is a widely prevalent disease worldwide, and with an increasingly ageing society, it has become a challenge for the field of regenerative medicine. OA is a disease process involving multiple joint tissues, including those not visible on radiography, and is a complex disease process with multiple phenotypes that require evaluation by a multimodality imaging assessment. The purpose of this study was to evaluate the effect of micro-fragmented fat tissue intra-articular injection 24 months after application in two ways: Indirectly using functional magnetic resonance imaging (MRI) assessment analyzing the glycosaminoglycans (GAG) content in cartilage by means of delayed gadolinium (Gd)-enhanced magnetic resonance imaging of cartilage (dGEMRIC), as well as clinical outcome on observed level of GAG using standard orthopedic physical examination including VAS assessment. In our previous study assessing comprehensive results after 12 months, the dGEMRIC results have drawn attention. The present study explores the long-term effect of intra-articular injection of autologous microfragmented adipose tissue to host chondrocytes and cartilage proteoglycans in patients with knee OA. A prospective, non-randomized, interventional, single-center, open-label clinical trial was conducted from January 2016 to April 2018. A total of 17 patients were enrolled in the study, and 32 knees were assessed in a 12-month follow-up, but only 10 patients of them with 18 knees are included in a 24-month follow-up. The rest of the seven patients dropped out of the study 12 months after follow-up: three patients underwent knee arthroplasty, and the remaining four did not fulfil the basic criteria of 24 months involvement in the study. Surgical intervention (lipoaspiration), followed by tissue processing and intra-articular injection of the final microfragmented adipose tissue product into the affected knee(s), was performed in all patients. Patients were assessed for a visual analog scale (VAS), dGEMRIC at the baseline, three, six, 12 and 24 months after the treatment. A magnetic resonance sequence in dGEMRIC due to infiltration of the anionic, negatively-charged contrast gadopentetate dimeglumine (Gd-DTPA2) into the cartilage indicated that the contents of cartilage glycosaminoglycans significantly increased in specific areas of the treated knee joint. Our results suggest that this method of single intra-articular injection of autologous microfragmented adipose tissue improves GAG content on a significant scale, with over half of the measurements suggesting relevant improvement 24 months after intra-articular injection opposed to the expected GAG decrease over the natural course of the disease.
Autism in Fragile X Syndrome; A Functional MRI Study of Facial Emotion-Processing
Authors: McKechanie Andrew G. , Campbell Sonya , Eley Sarah E. A. , Stanfield Andrew C.
Published: December 2019

Fragile X syndrome (FXS) is the most common inherited cause of intellectual disability and autism spectrum disorder, and among those with fragile X syndrome, approximately 1/3rd meet a threshold for an autism spectrum disorder (ASD) diagnosis. Previous functional imaging studies of fragile X syndrome have typically focused on those with fragile X syndrome compared to either neurotypical or autism spectrum disorder control groups. Further, the majority of previous studies have tended to focus on those who are more intellectually able than is typical for fragile X syndrome. In this study, we examine the impact of autistic traits in individuals with fragile X syndrome on a paradigm looking at facial emotion processing. The study included 17 individuals with fragile X syndrome, of whom 10 met criteria for autism as measured by the Autism Diagnostic Observation Schedule (ADOS). Prior to the scan, participants rehearsed on a mock scanner to help acclimatize to the scanner environment and thus allow more severely affected individuals to participate. The task examined the blood-oxygen-level-dependent (BOLD) response to fearful and neutral faces taken from the Ekman faces series. Individuals in the autism group had a region of significantly reduced activity centered on the left superior temporal gyrus, compared to those with FXS alone, in response to the fearful faces. We suggest that autism in individuals with fragile X syndrome is associated with similar changes in the neurobiology of facial emotion processing as seen in idiopathic autism.
Danio Rerio as Model Organism for Adenoviral Vector Evaluation
Authors: Gulías Paola , Guerra-Varela Jorge , Gonzalez-Aparicio Manuela , Ricobaraza Ana , Vales Africa , Gonzalez-Aseguinolaza Gloria , Hernandez-Alcoceba Rubén , Sánchez Laura
Published: December 2019

Viral vector use is wide-spread in the field of gene therapy, with new clinical trials starting every year for different human pathologies and a growing number of agents being approved by regulatory agencies. However, preclinical testing is long and expensive, especially during the early stages of development. Nowadays, the model organism par excellence is the mouse (Mus musculus), and there are few investigations in which alternative models are used. Here, we assess the possibility of using zebrafish (Danio rerio) as an in vivo model for adenoviral vectors. We describe how E1/E3-deleted adenoviral vectors achieve efficient transduction when they are administered to zebrafish embryos via intracranial injection. In addition, helper-dependent (high-capacity) adenoviral vectors allow sustained transgene expression in this organism. Taking into account the wide repertoire of genetically modified zebrafish lines, the ethical aspects, and the affordability of this model, we conclude that zebrafish could be an efficient alternative for the early-stage preclinical evaluation of adenoviral vectors.