bluuebunny/crossref_metadata_2025 huggingface dataset:
Expression of Integrin β1, Focal Adhesion Kinase, and PDZ-Binding Motif in Human Liver Cirrhosis and Simple Steatosis
Authors: Goli Zahra , Khodadadi Iraj , Karimi Jamshid , Mohagheghi Sina , Tavilani Heidar
Published: January 2023

Background: Integrins are transmembrane mechanosensitive proteins that negatively contribute to the pathogenesis of different types of chronic liver disease and can activate focal adhesion kinase (FAK). Objectives: This study aimed to determine the hepatic integrin β1 and FAK mRNA as well as the transcriptional coactivator with PDZ-binding motif (TAZ) protein expressions in cirrhotic patients and simple steatosis. Methods: In this case–control study, liver tissues were collected from 30 cirrhotic patients with various etiologies (i.e., nonalcoholic steatohepatitis-, primary sclerosing cholangitis-, alcoholic-, autoimmune hepatitis [AIH]- and hepatitis B virus [HBV]/hepatitis C virus [HCV]-related cirrhosis [six per group]), liver samples with simple steatosis (n=6), and control liver tissues (n=9). Results: Integrin β1 gene expression was significantly up-regulated in all cirrhotic groups compared to control group (P<0.05), with the exception of AIH cirrhosis. However, hepatic FAK gene expression and TAZ protein level in the cirrhotic groups were not significantly different than those in the control group. Furthermore, hepatic integrin β1 and FAK gene expressions as well as TAZ protein level in simple steatosis were significantly lower than those in nonalcoholic steatohepatitis (NASH) cirrhosis and control (P<0.05). Conclusion: Integrin β1 was up-regulated in cirrhotic liver tissues. In addition, FAK, integrin β1, and TAZ were concordantly down-regulated in simple steatosis, and may have been involve in the steatosis development.
Angiotensinogen Gene M235T and T174M Polymorphisms in Diabetic Nephropathy in a Bangladeshi Population
Authors: Rahman Md. Mizanur , Salim Shahidul Islam , Khan Imran , Hossain Khondoker Moazzem , Ali Liaquat , Hassan Zahid
Published: January 2023

Background: Marker gene polymorphisms linked with the renin-angiotensin-aldosterone system (RAAS) have been broadly studied in diabetic nephropathy (DN) patients considering that RAAS is a potential drug target to slow down kidney disease progression. Objectives: The aim of the present study was to determine the link between M235T and T174M variants of angiotensinogen (AGT) gene and DN. Methods: A total of 93 patients with DN, mean age of 56±8 years, systolic blood pressure (SBP) of 141±14, and diastolic blood pressure (DBP) of 84±7 mm Hg (mean±SD) were investigated, among whom 59 patients had a family history of type 2 diabetes mellitus. A total of 96 healthy subjects served as the control group with no family history of diabetic nephropathy (FHDN) and type 2 diabetes mellitus, a mean age of 47±10 years, SBP of 126±11, and DBP of 76±6 mm Hg. PCR–restriction fragment length polymorphism was employed for genotyping M235T and T174M molecular variants. Results: Genotype frequencies of the variants M235T (χ2=2.038, P=0.361) and T174M (χ2=2.952, P=0.229) did not show any statistically significant association with type 2 diabetic nephropathy (T2DN) compared to the control. Based on FHDN and family history of diabetes mellitus (FHDM), the frequency of genotypes of M235T marker (P=0.360) in FHDN, and (P=0.886) FHDM; T174M marker (P=0.641) in FHDN, and (P=0.425) FHDM also did not show any statistically significant association with T2DN compared to the controls. Conclusion: M235T and T174M variants were not associated with DN in a Bangladeshi population.
A Flippase-Like Domain-Containing Protein From Deltaproteobacteria Bacterium (Marine Sediment Metagenome) Has Anti-prostate Cancer Effects
Authors: Danaeifar Mohsen
Published: January 2023

Background: Prostate cancer is the second prevalent cancer and the fifth cause of deaths related to the men malignancies. The cancerous cell can travel from prostate to other parts of the body and cause metastasis in other organs such as bones and lymph nodes. For the treatment of the prostate cancer, chemotherapy and hormone therapy are being used. Hormone therapy in this disease is primarily based on the androgen suppression to prevent the growth and division of the cancerous cells. Different classes of drugs are used for hormone therapy. One group of these drugs is reducers of androgen production in adrenal glands. These drugs prevent the androgen production in adrenal glands and cancerous cells. Leuprorelin which is also known as leuprolide is a synthetic peptide that is used for treatment of prostate cancer. Objectives: The aim of this study is to explore novel anti-prostate cancer compounds using proteomics of microorganisms. Methods: Leuprorelin synthetic peptide was chosen as the template for amino acid sequence homology search using blast, and the resulting protein of the best candidate sequences were employed as a query to perform docking test against the gonadotropin-releasing hormone receptor (GRHR) using HDOCK web server. Results: By employing an in silico approach, natural products with structure and function similar to leuprorelin were explored, and their features were characterized. A flippase-like domain-containing protein from Deltaproteobacteria bacterium showed strong binding to gonadotropin-releasing hormone receptor GRHR with docking score of -425. The absorption, distribution, metabolism, and excretion (ADME) tests showed no potential toxicity of this natural product to the body. Conclusion: The present study demonstrated that the proteomics of living organisms contains natural compounds that can be considered a valuable source of medically important resources.
The Effect of Aqueous Extract of Gum Arabic on Hepato-renal Function During Ethanol Withdrawal Induced Stress in Wistar Rats
Authors: Omoruyi Jeffrey I. , Eriyamremu George E. , Ebhohimen Israel E. , Emuedo Oke. A. , Uzunuigbe Edwina O.
Published: January 2023

Background: Alcohol withdrawal syndrome (AWS) is a life-threatening condition affecting alcoholics who ceased or decreased their alcohol consumption. The synthetic drugs used to manage these consequences are not without undesirable effects; hence, the need for a natural and affordable approach is raised. Objectives: This study aimed at investigating the effect of aqueous extract of gum arabic (GA) on hepato-renal functions during ethanol withdrawal syndrome in Wistar rats. Methods: In phase I, dose-response for GA and alcohol for the study were determined. In phase II, the effect of GA on biomarkers during AWS was studied. A total of 60 male Wistar rats were used for the study. Blood and tissue samples were obtained at the end of stipulated periods of oral administration for biochemical and histological analysis, and biochemical parameters were analyzed by spectrophotometry. Results: In the dose-response study, there were no significant differences (P≥0.05) in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities as well as in total bilirubin (TBIL), malondialdehyde (MDA), sodium ion (Na+), potassium ion (K+), and creatinine concentrations in groups treated with 200 mg/kg body weight (bw) and 400 mg/kg bw GA aqueous extract compared to the control group. However, significant alterations were observed in groups treated with 600 and 800 mg/kg bw GA extract. Furthermore, rats that received 5.5 mL/kg bw alcohol showed marked changes in biochemical parameters compared to the group that received 4.5 mL/kg bw and the control group. The results obtained in Phase II exhibited the hepato-renal protective effect of GA during ethanol withdrawal. Statistical analysis of the obtained results indicated a better response from the study groups that were pre-treated or co-administered with GA compared to the group that was post-treated. Conclusion: The result of this study suggests that GA aqueous extract offered better protection prophylactically than curatively.
Investigating Preventive Effect of Vitamin D and N-acetylcysteine Against Kidney Injury in Rats Versus the Promotive Effect of Paraquat
Authors: Felehgari Hamed , Khiripour Nejat , Mehri Fereshteh , Soleimani Asl Sara , Ahmadimoghaddam Davoud , Ranjbar Akram
Published: January 2023

Background: Paraquat (PQ) is one of the most important herbicides used in agriculture. Objectives: This study was conducted to compare the preventive effect of vitamin D (Vit D) and N-acetylcysteine (NAC) against kidney injury in rats versus the promotive effect of PQ. Methods: In this study, rats were divided into six groups. The control group (group 1) received normal saline, Vit D group (group 2) received intraperitoneal (IP) injections of+Vit D (2 μg/kg), NAC group (group 3) received NAC (6.25 mg/kg, IP), PQ group (group 4) received PQ (5 mg/kg/d, IP), PQ+Vit D group (group 5) received PQ+Vit D (5 mg/kg/d+2 μg/kg/d, IP) and PQ+NAC group (group 6) received PQ+NAC (5 mg/kg/d+6.25 mg/kg/d, IP). The animals were treated for 7 consecutive days as a sub-chronic exposure. After the collection of urea and serum creatinine, biomarkers of oxidative stress and kidney histopathology were investigated. Results: PQ increased lipid peroxidation (LPO), urea, and serum creatinine, but it significantly decreased total antioxidant capacity (TAC) and thiol groups. In the groups treated with Vit D and NAC, it was observed that LPO, urea, and creatinine significantly decrease compared with the PQ group, and TAC, thiol groups, and Vit D levels increased in kidney tissue. Conclusion: The obtained findings revealed that both Vit D and NAC used as preventive compound were able to reduce oxidative stress and tissue damage caused by PQ toxicity in the kidney.
Evaluation of Acute and Subacute Toxicity of Fumaria officinalis Alkaloids in Mice
Authors: Yahiaoui Sonia , Khamtache-Abderrahim Sabiha , Otmani Amar , Bachir-bey Mostapha , Kati Djamel-Edine , Lequart-Pillon Michelle , Gontier Eric , Maiza-Benabdesselam Fadila
Published: January 2023

Background: Fumaria officinalis is largely used in traditional medicine due to its efficiency in the treatment and prevention of numerous diseases and its large spectrum of therapeutic effects. Its multiple beneficial properties are due to its richness in bioactive substances, particularly isoquinoline alkaloids. However, few studies have addressed the toxicity of this plant. Objectives: The present work aimed to study acute and subacute toxicity of alkaloids extracted from F. officinalis using Swiss albino mice as the in vivo model. Methods: Alkaloids from the aerial parts of F. officinalis were extracted and administered to male and female Swiss albino mice. The acute and subacute toxicities were studied by monitoring the weight and histopathological study of animal bodies and organs (e.g., liver, heart, spleen, and kidneys). Results: The results revealed that mice treated with increasing doses developed serious symptoms of toxicity (i.e., respiratory problems, tremors, coma, and paralysis leading the death) and lost weight. The LD50 was estimated at 1341.11 mg/kg permitting its classification as a low-toxic plant. The microscopic observations demonstrated disturbances in the kidney and liver, but not the heart and spleen. Conclusion: The alkaloids of the aerial parts of F. officinalis expressed severe toxicity in mice, particularly at high doses. Nevertheless, the neutral fraction of alkaloids is more indicated.
The Relationship Between Some Laboratory Findings and the Prognostic Significance of Phosphorus and 25-(OH) Vitamin D3 Values in SARS-CoV-2 Cases
Authors: Keskin Adem , Aci Recai
Published: January 2023

Background: There are many articles in the literature about the importance of 25-(OH) vitamin D3 in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases. However, there is no consensus on 25-(OH) vitamin D3 to predict the prognosis of SARS-CoV-2 cases. Additionally, phosphorus, which has an important role in the cytokine storm, contributes to poor prognosis in SARS-CoV-2 cases. Objectives: This study aimed to examine phosphorus and 25-(OH) vitamin D3 levels to predict the prognosis of COVID-19. Methods: A total of 230 SARS-CoV-2 cases and 230 healthy people were included in this study. ROC curve analysis was performed for combined evaluation of phosphorus and 25-(OH) vitamin D3 levels. Results: In the ROC curve analysis, the area under curve (AUC) was 0.9282, the sensitivity was 92.70%, and the specificity was 92.80%. Phosphorus and 25-(OH) vitamin D3 levels showed negative correlations with procalcitonin, ferritin, D-dimer, C-reactive protein, neutrophil-lymphocyte ratio, and monocytelymphocyte ratio. Conclusion: Low phosphorus and 25-(OH) vitamin D3 levels may be indicators for poor prognosis in SARS-CoV-2 cases. Conversely, normal phosphorus and 25-(OH) vitamin D3 levels may be indicators for a good prognosis in SARS-CoV-2 cases.
Designing Potent Anticancer Peptides by Aurein 1.2 Key Residues Mutation and Catenate Cell-Penetrating Peptide
Authors: Salarpour Garnaie Hamta , Shahabi Arman , Geranmayeh Mohammad Hossein , Barzegar Abolfazel , Yari Khosroushahi Ahmad
Published: January 2023

Purpose: Aurein 1.2 (Aur) peptide is known for possessing anticancer characteristics devoid of conventional therapeutics side effects. For improving Aur peptide anticancer functionality, different anticancer peptides were constructed based on Aur peptide through targeting two separate strategies, including (1) sequence-based mutations and (2) adding a cell-penetrating peptide linker. Methods: The study was approached by designing three different analogs of Aur, including (a) Aur mutant (Aurm), (b) Aur with N-terminal polyarginine linker (R5-Aur), and (c) Aurm with R5 (R5-Aurm). Computational molecular dynamics simulations clearly showed higher structural stability of R5-Aur and R5-Aurm compared to Aur, solely. The α-helical properties of R5-Aur and R5-Aurm were protected during 500 ns simulations in water solution while no such structural conservation was seen for Aur in silico. Results: The results of the current study highlight response to one of the main challenges of cancer therapy through selective invasion of Aur to cancer cells without significant involvement of normal cells. This issue was confirmed by different assays, including: MTT assay, flow cytometry, qPCR, and nuclei morphological observations. Furthermore, this study intensifies exploiting in silico approaches for adjusting drug delivery. The results of different assessments on designed peptides reveal an anticancer activity pattern rising from Aur toward Aurm, and R5- Aur, consecutively. Conclusion: The designed structure of Aur shows improved anticancer activity through molecular changes which makes it suggestable for anticancer therapies.
Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam
Authors: Valizadeh Hadi , Mahdinloo Somayeh , Zakeri Negin , Sarfraz Muhammad , Nezafat Saeed , Zakeri-Milani Parvin
Published: January 2023

Purpose: Poor aqueous solubility hampers the development of several compounds as pharmacological agents. Hence, preparing novel formulations with augmented absorption is a challenge in pharmaceutical industries. In this paper, we have examined the effect of basic amino acids including arginine (ARG), lysine (LYS), and glucosamine (GlucN) on the solubility of ibuprofen (IBU) and piroxicam (PXM) as drugs with limited solubility. We have also studied the effect of the dissolution media with the pH values 1.2 to 7.4. Methods: The saturation shake-flask method was used for solubility studies in the presence of amino acids. Briefly, buffer solutions containing different concentrations of amino acids were prepared. Then, an excess amount of each drug with these buffers was shaken to reach equilibrium. After 48 hours, the upper phase was separated, and solubility was calculated by reading their UV-Vis absorbance. Results: The results illustrated that amino acids increased solubility of both drugs with different ratios, which were pH and concentration-dependent. Solubility improved as the amount of amino acids went up, and this upward pattern was more robust with ARG than LYS. The presence of GlucN in citrate buffer significantly enhanced IBU solubility. The solubility of PXM in accompany of GlucN in water did not change significantly while in citrate buffer solubility enhanced specially at pH 6. Conclusion: Overall, GlucN in citrate buffer and ARG in phosphate buffer could be introduced as the most suitable media for IBU and PXM solubility improvement, respectively.
Inhibitory effects of mixed flavonoid supplements on unraveled DSS-induced ulcerative colitis and arthritis
Authors: Panda Siva Prasad , Mahamat Mahamat  Sami Adam , Rasool Malikyahia Abdul , Prasanth DSNBK , Ismail Idris Adam , Abasher Moyed Abasher Ahmed , Jena Bikash Ranjan
Published: January 2023

Introduction: The mixed flavonoid supplement (MFS) [Trimethoxy Flavones (TMF) + epigallocatechin-3-gallate (EGCG)] can be used to suppress inflammatory ulcers as an ethical medicine in Ayurveda. The inflammation of the rectum and anal regions is mostly attributed to nuclear factor kappa beta (NF-κB) signaling. NF-κB stimulates the expression of matrix metalloproteinase (MMP9), inflammatory cytokines tumor necrosis factor (TNF-α), and interleukin-1β (IL-1β). Although much research targeted the NF-κB and MMP9 signaling pathways, a subsequent investigation of target mediators in the inflammatory ulcer healing and NF-κB pathway has not been done. Methods: The docking studies of compounds TMF and EGCG were performed by applying PyRx and available software to understand ligand binding properties with the target proteins. The synergistic ulcer healing and anti-arthritic effects of MFS were elucidated using dextran sulfate sodium (DSS)-induced colon ulcer in Swiss albino rats. The colon mucosal injury was analyzed by colon ulcer index (CUI) and anorectic tissue microscopy. The IL-1β, tumor necrosis factor (TNF-α), and the pERK, MMP9, and NF-κB expressions in the colon tissue were determined by ELISA and Western blotting. RT-PCR determined the mRNA expression for inflammatory marker enzymes. Results: The docking studies revealed that EGCG and TMF had a good binding affinity with MMP9 (i.e., -6.8 and -6.0 Kcal/mol) and NF-kB (-9.4 and 8.3 kcal/mol). The high dose MFS better suppressed ulcerative colitis (UC) and associated arthritis with marked low-density pERK, MMP9, and NF-κB proteins. The CUI score and inflammatory mediator levels were suppressed with endogenous antioxidant levels in MFS treated rats. Conclusion: The MFS effectively unraveled anorectic tissue inflammation and associated arthritis by suppressing NF-κB-mediated MMP9 and cytokines.
Multi spectroscopic and molecular simulation studies of propyl acridone binding to calf thymus DNA in the presence of electromagnetic force
Authors: Sharifi-Rad Atena , Amiri-Tehranizadeh Zeinab , Talebi Atiye , Nosrati Niknaz , Medalian Morvarid , Pejhan Mahtab , Hamzkanloo Nazanin , Saberi Mohammad Reza , Mokaberi Parisa , Chamani Jamshidkhan
Published: January 2023

Introduction: Here, the interaction behavior between propyl acridones (PA) and calf thymus DNA (ct-DNA) has been investigated to attain the features of the binding behavior of PA with ct-DNA, which includes specific binding sites, modes, and constants. Furthermore, the effects of PA on the conformation of ct-DNA seem to be quite significant for comprehending the medicine’s mechanism of action and pharmacokinetics. Methods: The project was accomplished through means of absorbance studies, fluorescence spectroscopy, circular dichroism, viscosity measurement, thermal melting, and molecular modeling techniques. Results: The intercalation of PA has been suggested by fluorescence quenching and viscosity measurements results while the thermal melting and circular dichroism studies have confirmed the thermal stabilization and conformational changes that seem to be associated with the binding. The binding constants of ct-DNA-PA complex, in the absence and presence of EMF, have been evaluated to be 6.19 × 104 M-1 and 2.95 × 104 M-1 at 298 K, respectively. In the absence of EMF, the ∆H0 and ∆S0 values that occur in the interaction process of PA with ct-DNA have been measured to be -11.81 kJ.mol-1 and 51.01 J.mol-1K-1, while in the presence of EMF they were observed to be -23.34 kJ.mol-1 and 7.49 J.mol-1K-1, respectively. These numbers indicate the involvement of multiple non-covalent interactions in the binding procedure. In a parallel study, DNA-PA interactions have been monitored by molecular dynamics simulations; their results have demonstrated DNA stability with increasing concentrations of PA, as well as calculated bindings of theoretical ΔG0. Conclusion: The complex formation between PA and ct-DNA has been investigated in the presence and absence of EMF through the multi spectroscopic techniques and MD simulation. These findings have been observed to be parallel to the results of KI and NaCl quenching studies, as well as the competitive displacement with EB and AO. According to thermodynamic parameters, electrostatic interactions stand as the main energy that binds PA to ct-DNA. Regarding the cases that involve the Tm of ct-DNA, EMF has proved to increase the stability of binding between PA and ct-DNA.
Dual-targeting of brain tumors with nanovesicles
Authors: Kianinejad Nazanin , Kwon Young Min
Published: January 2023

The delivery of chemotherapies to brain tumors faces the difficult task of crossing the blood-brain barrier (BBB).1-4 The brain capillary endothelial cells (BCECs) along with other cell lines, such as astrocytes and pericytes, form the BBB. This highly selective semipermeable barrier separates the blood from the brain parenchyma. The BBB controls the movement of drug molecules in a selective manner5 and maintains central nervous system (CNS) homeostasis. Depending on the properties of drugs such as their hydrophilic-lipophilic balance (HLB), some can cross the BBB through passive diffusion.6 However, this approach alone has not led to successful drug developments due to low net diffusion rates and systemic toxicity. Although the use of nanomedicine has been proposed to overcome these drawbacks, many recent studies still rely on the so-called ‘enhanced permeability and retention (EPR)’ effect though there is a realization in the field of drug delivery that EPR effect may not be sufficient for successful drug delivery to brain tumors. Since, compared to many other solid tumors, brain tumors pose additional challenges such as more restrictive blood-tumor barrier as well as the well-developed lymphatic drainage, the selection of functional moieties on the nanocarriers under consideration must be carried out with care to propose better solutions to this challenge.
Establishing the Mortality Rate of under Five-Year-Old Children by Capture-Recapture Method
Authors: Pezhhan Ali , Jahanmehr Safiyeh
Published: January 2023

Background. Under count is a common phenomenon in health systems. One of the methods used for sensitivity identification of care system or identification of cases in registry is capture recapture method. Using two resources, this study aimed to estimate the actual mortality rates of under five-year-old children. Methods. This study was conducted by capture-recapture method (two resources), using the recorded data in death cause systems of the Iranian Ministry of Health and Medical Education (MOHME) and Treatment and Registry Office. The population included all children aged five years in Tabriz, Iran. We also considered all the death cases in the target population from 2020.03.20 to 2021.03.20. The death cases were extracted from two resources and entered into the Microsoft Excel software. After removing duplicate cases with a special formula, we obtained the actual mortality rates using capture-recapture method with 95% confidence interval (CI) and estimated the actual mortality rates and death cases. Results. In 2020, the death rate of under five-year-old children was calculated to be 344 cases (338 to 350 with 95% CI). The sensitivity rate of causal registry system for all death cases in the target population was 43.17%. Also, the mortality rate of under one-year-old children was calculated as 275 cases (271 to 280 with 95% CI). While the sensitivity rate of causal death for the mortality of under one-year-old children was 92.72%, it was 82.28% for the Registry Office. Conclusion. A combination of different software for death information registry and the registration of information in the occurrence location could be efficient techniques to reduce under count registrations. It is believed that better and more reliable information could be obtained on death case numbers through using two lists and capture-recapture method compared to using one of the two resources.
Medical Students’ Self-Assessment of Communication Skills During COVID-19 Pandemic at Tabriz University of Medical Sciences
Authors: Baradaran Binazir Maryam , Heidari Fariba
Published: January 2023

Background. In addition to patient satisfaction and treatment compliance by patients, appropriate physician-patient communication leads to development of a relationship between physicians and patients based on trust. The purpose of this study was self-assessment of medical interns and stagers of Tabriz University of Medical Sciences in communicating with the patients during COVID-19 pandemic. Methods. The population of this cross-sectional study included all medical stagers and interns at Tabriz University of Medical Sciences, Iran from November to December, 2020. The research tool was a standard questionnaire of Interpersonal Communication Skills Scale developed by Vakili et al. to assess interpersonal communication skills. After the online questionnaires were filled by the students, we calculated and compared the mean score of self-assessment of communication skills for the medical stagers and interns. Additionally, we compared the mean scores of students’ self-assessment in term of educational level, gender, wards and on duty hours during a week. Results. In this study, a total of 152 medical stagers and interns participated. More than half of the participants were female (55.92%). The mean self-assessment score of the participants was 93.83±9.78. The mean self-assessment score of medical stagers and interns was 93.15±8.13 and 94.51±12.26, respectively. There was no statistically significant difference in self-assessment score of patient communication based on educational level and ward (surgical and non-surgical), but the mean score of self-assessment was significantly higher in females (P=0.044). Conclusion. Most medical interns and stagers had an average self-assessment score regarding patient communication during COVID-19 pandemic. So, appropriate training can improve medical students’ communication skills. However, the communication skills required for medical students may vary depending on whether they are dealing with a pandemic or non-pandemic situation.
A Review of Critical Thinking Training in Librarianship, Knowledge, and Information Science
Authors: Esmaeilzad Hojbi Sima , Gavgani Vahideh Zarea , Zarei Atefeh , Familrouhany Seyed Aliakbar
Published: January 2023

Critical thinking, an important construct in the educational dimension, is an active process which uses a specific set of skills and enables one to make appropriate decisions and discriminate between information received. Therefore, critical thinking is considered as an important aspect of information performance in library education. In the field of library and information science, so far, no coherent study has investigated the possible aspects of teaching critical thinking. Hence, this narrative study aimed to review the available literature to determine if critical thinking is considered in the librarianship education and research. To this end, four electronic databases, including Medline (PubMed), ERIC, ProQuest, and Scopus were searched using the following keywords: Critical Thinking; Thinking; Education; Curriculum, Teaching; Librarianship; Library and Information Science; Medical Library and Information Science; and Knowledge and Information Science. A total of 439 articles were identified. After the initial review of the titles and abstracts and the removal of irrelevant and repetitive articles, 67 articles entered the evaluation stage. After reviewing the full text of the remaining articles, 32 articles were eligible to enter the study. Based on our review, the literature on the critical thinking in library science could be categorized into four classes. Moreover, critical thinking should be improved through using different educational strategies and be integrated into education curricula.
Evaluation of Flipped Classroom Teaching Methods in Improving the Knowledge and Readiness of Medical Students in Disasters
Authors: Mohammadinia Leila , Amini Mitra , Marzieh Alamolhoda , Faeze Bashiirii
Published: January 2023

Background. Flipped classroom, as one of the new educational techniques, can lead to effective clinical practice. Applying this method to basic lessons provides for students’ more in-depth learning. Therefore, this study examines flipped classroom teaching method in improving the knowledge and readiness of medical students in disaster management. Methods. This quasi-experimental single-group pretest-posttest study included a total of 106 medical students at Shiraz University of Medical Sciences, Iran. The participants were selected from the students admitted to the course in two consecutive years 2018 and 2019. Different teaching methods were used to cover the topics based on PowerPoint, clip showing, small group work, discussion, and question sessions. Data analysis was performed using paired t-test and Wilcoxon non-parametric test. Results. Based on the results, out of 106 participants (65 or 61.3% females vs. 41 or 38.7% males), 85 students completed the course. The age group of students was 19-39 years (mean age: 20.29±3.27). There was no significant relationship between gender and readiness in terms of knowledge and attitude (P≥0.05). There was a significant difference between attitude and level of preparation before and after the test. The average level of knowledge, attitude, and readiness in response to risks in the post-test compared to the pre-test (after teaching upside down) increased by 1.93%, 3.82%, and 3.35%, respectively. Conclusion. The importance of disasters requires the growth of awareness and preparedness. Teaching flipped classroom for the concepts of disasters can create more dynamism in students and improve their knowledge and preparedness to deal with disasters.
Effect of Online Training of Emotion Regulation in Terms of Locus of Control on Medical Students’ Anger Control and Impulsiveness: A Quasi-Experimental Study
Authors: Khonya Yousef , Narimani Mohammad , Aghajani Seyfullah , Hajloo Nader
Published: January 2023

Background. Lack of anger control and impulsiveness can lead to different personal and social harms. This study aimed to investigate the effect of emotion regulation training according to the source of control on anger control and impulsiveness of medical students. Methods. This quasi-experimental research was conducted with pre-test post-test design, including a control group with follow-up. The population consisted of general medicine students studying basic sciences and physiopathology. After completing three questionnaires of anger control, impulsivity, and locus of control, 60 students were randomly selected and assigned to experimental and control groups. The participants in the experimental group received eight sessions of emotion regulation training. The questionnaires were administered to all participants after training in two sessions of post-test and follow-up post-test. The data were analyzed using multivariate analysis of variance (MANOVA). The data were analyzed through two-factorial multivariate analysis of covariance (MANCOVA) using the Statistical Package for the Social Sciences (SPSS) v. 24. Results. Emotion regulation training and its interaction with the locus of control had a positive effect on anger and impulsiveness control. Conclusion. Training emotion regulation and other social skills to university students can help them face the challenges during their studies.
Investigating and Presenting the Optimal Alternative for Managing and Disposing Landfill Leachate in Iran Using the Analytical Hierarchy Process
Authors: Taghipour Hassan , Seyed Mousavi Seyedeh Maryam
Published: January 2023

Background. In most developing countries, concern about leachate pollution is serious. This study aimed to determine the best alternative for leachate management and disposal using the Analytic Hierarchy Process (AHP) method. Methods. In this descriptive-applied research, the criteria and alternatives for leachate management and disposal were selected, including evaporation pond (natural with sunlight), recirculation/evaporation, and treatment (physical and chemical, biological/advanced). Then, three criteria and 11 sub-criteria were selected. The criteria and sub-criteria were weighted, using the Expert Choice 11 software and compared two by two to determine the relative importance of each. Then, each of the alternatives was compared and prioritized two by two in relation to the criteria and sub-criteria. Results. The results of the study showed that the evaporation pond (natural with sunlight) was the most preferable alternative with an importance coefficient of 0.569. The alternative of recycling/evaporation and treatment (physical and chemical, biological/advanced) with an importance factor equal to 0.216 was placed in the next priority. The consistency ratio (CR) of all the paired matrices of the alternatives was equal and less than 0.1. Conclusion. By considering the economic, technical, operating, health, and environmental criteria, the evaporation pond method (natural with sunlight) was chosen as the best method for managing and disposing landfill leachate in Iran. Therefore, it is recommended to consider the alternative of leachate management in landfills, especially considering the climatic conditions of the country (90% dry and semi-arid climate).
The Experiences of Health Transformation Plan in Southeast Iran: A Qualitative Study
Authors: Zanganeh Baygi Mehdi , Peyvand Mostafa
Published: January 2023

Background. Considering the need for new health programs, the Health Transformation Plan, as one of the largest health system reform programs, has been implemented in Iran since 2014. Methods. This cross-sectional, applied study was conducted qualitatively on 27 participants in 2019 (19 service providers and 8 service recipients). The data collection tool was a semi-structured interview guide that was validated through a comprehensive literature review, and consultation with health strategy transformation experts. The research plan was approved by the ethics committee and the research work commenced accordingly. The research work was carried out at the research participants’ workplace (employees) via interviewing process. All the interviews were recorded daily. The data interpretation and analysis were conducted through coding and content analysis approach. Results. The data analysis for participants’ groups (i.e., service givers, human resources, infrastructure, education, and service recipients), using the open coding stage and the initial concept in the sub-coding stage, indicated 10 minor and 5 major categories. Conclusion. Appropriate education, quality of services, financial support, and specialized manpower can be considered as possible ways to cope with the challenges in the health transformation plan.
An Overview on Electrochemical Approaches for the Detection of Alpha-Fetoprotein
Authors: Golsanamlu Zahra , Soleymani Jafar
Published: January 2023

Cancer biomarkers have a remarkable role for not only early-stage detection of cancer but also in the monitoring therapy efficacy. Alpha-fetoprotein (AFP) is one of the biomarkers which is highly expressed in hepatocellular carcinoma (HCC) patients. Therefore, a high level of AFP can be considered for early diagnosis of HCC. Electrochemical assays can be used as the miniaturized biosensors for real-time detection of the analytes which are mostly categorized into three groups for AFP detection including immunosensors, aptasensors, and molecularly imprinted polymer-based sensors. All of these techniques provide beneficial detection features of fast and cost-effective compared with other analytical methods. This review describes the most recent electrochemical platforms for AFP detection to provide a perspective for future studies.
Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples
Authors: Mohammadzadeh Sedigheh , Karimzadeh Zahra
Published: January 2023

Background: Fentanyl is an opioid analgesic drug extensively used to alleviate pain with no consciousness loss. To address the fentanyl crisis from the criminal and medical perspectives,the development of a free drug determination methodology for this drug is crucial. Methods: A fluorescent nanoprobe based on copper sulfide nanoparticles (CuS NPs) is developed for the determination of fentanyl in exhaled breath condensate (EBC). NPs are synthesized according to a hydrothermal method and their size and morphology are characterized via X-ray diffraction (XRD), Fourier-transformed infrared (FTIR), and TEM. The fluorescence intensity of the nanoprobe is enhanced in the presence of fentanyl. The affinity of CuS NPs to complex formation with fentanyl results in blocking non-radiative e−/h+recombination defect sites on the surface of NPs and consequently enhancing the signal intensity. One at a time optimization method was used for the optimization of reaction conditions. Results: Under the optimized conditions, a low limit of detection (LOD) of 0.008 µg mL-1 was obtained for fentanyl determination. Furthermore, a linear relationship is found between the analytical response and the concentration of fentanyl in the range of 0.01-2.0 µg mL-1 with a relative standard deviation of<2.5%. Conclusion: The validated method is applied for the determination of fentanyl in the EBC of patients receiving fentanyl treatment.
Multi-drug Resistant Escherichia Coli from Poultry: A Potential Source of Antimicrobial Resistance Spread in Poultry Farm Environment
Authors: Ugwu Ifeoma Chinyere , Wilson Francis , Ugwu Chidozie Clifford
Published: January 2023

Background: Escherichia coli is known to inhabit the gastrointestinal tract of poultry and other animals. E. coli infection can cause major economic losses in poultry production. The development of resistance to the commonly used antimicrobials in the treatment of such infection can be a setback in the poultry sector. Objective: This study was carried out to determine the antibiotic resistance profile of E. coli isolated from chickens in Imo State, Nigeria. Materials and Methods: Twelve poultry farms were selected across the 3 senatorial zones of the state using purposive random sampling. A total of 120 cloacal samples were collected from chickens using sterile swabs. The samples were streaked onto MacConkey agar (MCA) and incubated at 37o C overnight. Pink colonies on the MCA were streaked onto eosin-methylene blue agar, incubated for 24 hours at 37o C, and confirmed by indole, methyl red, Voges-Proskauer, and citrate (IMVIC) tests. Antimicrobial susceptibility test was carried out using disc diffusion technique, and the inhibition zone diameter was measured and recorded as resistant or susceptible. Results: Twenty isolates out of the 120 samples were identified as E. coli. Twenty isolates were highly resistant to 5 antibiotics out of 10 antibiotics used. Antibiotic resistance patterns were as follows: amoxicillin (AMP) (95%), cephalothin (CEP) (90%), nalidixic acid (NA) (85%), trimethoprim (SXT) (85%), cefoperazone (PEF) (80%), ampicillin (AMP) (70%) ofloxacin (OFX) (15%), ciprofloxacin (CIP) (10%), gentamicin (CN) (10%), and streptomycin (S) (10%). Sixteen resistance patterns were recorded with AMC-SXT-AMP-CEP-NA-PEF being the most prevalent. Conclusion: This study shows that multi-drug resistant E. coli strains are present in poultry farms in Imo State.
Detection of Campylobacter coli and Campylobacter jejuni in Fecal Samples From Domestics and Livestock Employees
Authors: Yaslianifard Somayeh , Shahtaheri Zahrasadat , Qorbani Mostafa , Tavakoli Mahnaz , Mohammadzadeh Mohammad
Published: January 2023

Background: Campylobacter jejuni and Campylobacter coli are two zoonotic pathogens that commonly colonize the digestive tract of animal sources, including poultry, dogs, cats, and other various domestic farm animals. Colonization can lead to acute bacterial gastroenteritis in humans after close contact with animals or through the consumption of contaminated food or water. Objective: The present study aimed to detect and identify these bacteria in the fecal samples of domestic and livestock employees. Materials and Methods: A total of 96 samples were collected, including 48 sheep faces and 48 livestock workers’ stool samples. DNA was extracted from the samples, and the presence of Campylobacter species was investigated by the polymerase chain reaction due to the detection of specific marker genes. Results: Based on the PCR on hippuricase gene (hipO) and aspartokinase gene (asp), C. jejuni was found in 7 (7.3%) and C. coli in 4 (4.2%) of 96 isolates. Analysis of nucleotide sequences of PCR amolicons showed the genetic similarity of isolates from a common livestock center. Conclusion: Direct animal-to-human contamination seems unlikely. However, the contamination of farm water sources and sheep products with Campylobacter species can be a potential risk to human health.
Detection of Autotransporter Genes and Evaluation of the Relationship Between the Autotransporters and Biofilm Formation in Uropathogenic Escherichia Coli Isolated From Urinary Tract Infections
Authors: Asadi Karam Mohammad Reza , Habibi Mehri
Published: January 2023

Background: Uropathogenic Escherichia coli (UPEC) are among the pathogens causing urinary tract infections (UTIs). UPEC strains utilize autotransporter (AT) proteins for pathogenesis, especially the development of biofilm in the urinary tract. Objective: There is no general data about the frequency of ATs among the UPECs, thus we decided to assess the frequency and AT patterns in patients with UPEC recovered from UTI, as well as to find the possible correlation between the ATs and biofilm. Materials and Methods: Totally, 200 Escherichia coli were recovered from the urine of outpatients and inpatients with UTIs in Iran. Biofilm production was assessed by a phenotypic method. Extraction of DNA was done using the boiling method, and the extracted products were used for polymerase chain reaction (PCR). Amplification of AT genes was done by PCR with an optimized program in a thermal cycler apparatus. Then, the amplified genes were evaluated by electrophoresis. Results: Among the UPEC isolates, 63.5% and 36.5% were isolated from cystitis and pyelonephritis patients, respectively. In addition, the majority of UPECs (66.5%) were associated with hospitalized patients. Totally, 92% of isolates produced biofilm, of which 53.5%, 21.5%, and 17% were weak, moderate, and strong biofilm producers, respectively. Among investigated ATs, the most frequent genes were upaH (89%), upaB (87%), upaG (79%), vat (78%), sat (69%), upaC (67%), and ag43 (37%). There were 37 AT patterns among UPECs, and pattern 1 with the presence of all AT genes was the most common. It was found that the prevalence of upaB and upaG was significantly higher among the cystitis isolates compared to pyelonephritis isolates. Further, no significant difference was observed between the AT genes, except for upaC (P=0.003), and the intensity of biofilm formation in UPEC isolates. Conclusion: According to the high prevalence of AT genes among UPECs isolated from inpatients and outpatients, these factors can be ideal vaccine candidates for designing effective vaccines against the isolates. Evaluation of the efficacy of these ATs against UPECs is under investigation.
Diabetes and Herpes Zoster Association: A systematic Review and Meta-analysis
Authors: Razizadeh Mohammad Hossein , Khatami Alireza , Pourrostami Kumars , Bokharaei-Salim Farah , Zibaei Mohammad
Published: January 2023

Background: Diabetes mellitus is known to be a potential risk factor for herpes zoster (HZ). The aim of the present study was to investigate the relationship between diabetes and HZ statistically. What is the status of diabetes in people with HZ, and are diabetes and HZ associations statistically significant? Answering these questions is the main purpose of this study. Methods: Systematic search was carried out in four major international databases, including PubMed, Scopus, Web of Science, and Google scholar for eligible records between January 2000 and December 2020. The overall prevalence of diabetes in HZ people, studies heterogeneity, as well as geographical distribution were estimated by a random-effect model applied in comprehensive meta-analysis (V2, BioStat) software. Results: Ultimately, 28 studies (29 datasets) were included in the present meta-analysis. The overall prevalence of diabetes in HZ people was estimated to be 12.7% (95% CI: 11.5%- 14%), the highest and lowest prevalence rates were 17.4% and 4% in Southeast Asian and American regions, respectively. Additionally, the odds ratio (OR) results suggested a significant association between HZ and diabetes (OR: 1.28, 95% CI: 1.18-1.38). Conclusion: The results indicate a significant association between HZ and diabetes, and this association should not be neglected. Future studies may reflect the effect of vaccination more seriously by considering this association.
Evaluation of Ocimum bascilicum Oil and Diethyltoluamide Cream as Repellent Against Female Anopheles Mosquitoes in Sinnar State of Sudan (2022)
Authors: Ibrahim Shadia Ahmed M. , Ishag Gawaher Gabl A. , Gasim Mohamed Awn Elsharif , Alhaj Halima Abd Elgader , Ahmed Hafiz Hassan Shebeir , Hamad Mosab Nouraldein Mohammed
Published: January 2023

Introduction: Female Anopheles mosquitoes are the exclusive vectors of malaria parasites in Sudan, along with several metazoan parasites such as filarial worms and viruses. The sense of smell is one of the most important senses that mosquitoes use for long-range host-seeking. Carbon dioxide from the breath of humans and animals is a strong attractant of female mosquitoes. Methods: This correlational study was performed in Al-Qaddal Center for Malaria Research in Sinnar State, Sudan. In this study, individual volunteers were topically applied Ocimum bascilicum oil to test its repellency activity. O. bascilicum oil was tested at four different concentrations (30%, 50%, 70%, and 100% v/v), respectively. Results: Based on the obtained results, the oil exhibited the highest repellency activity at 100% concentration, and the average repellency rate was 100%. However, the lowest repellency activity was at 30% concentration, and the average repellency rate was 45%. Diethyltoluamide 13% (Soffell cream) was tested as a reference repellent cream and showed the same effect as O. bascilicum oil at 70% concentration, and the average repellency rate was 94%. Conclusion: The results demonstrated that O. bascilicum oil had high repellency activity against female Anopheles mosquitoes, and thus can be used as mosquito natural repellent.