bluuebunny/crossref_metadata_2025 huggingface dataset:
The Flow of the Berry Curvature Vector Field
Authors: Stejskal Ondřej , Veis Martin , Hamrle Jaroslav
Published: October 2021

The concept of Berry phase and Berry curvature has become ubiquitous in solid state physics as it relates to variety of phenomena, such as topological insulators, polarization, and various Hall effects. It is well known that large Berry curvatures arise from close proximity of hybridiz-ing bands, however, the vectorial nature of the Berry curvature is not utilized in current research. On bulk bcc Fe, we demonstrate the flow of the Berry curvature vector field which features not only monopoles but also higher dimensional structures with its own topological features. They can provide a novel unique view on the electronic structure in all three dimensions. This knowledge is also used to quantify particular contributions to the intrinsic anomalous Hall effect in a simple analytical form.
H3K27M-mutant Diffuse Midline Gliomas should be Further Molecularly Stratified: An Integrated Analysis of 669 Patients
Authors: Vuong Huy Gia , Le Hieu Trong , Ngo Tam N.M. , Fung Kar-Ming , Battiste James D. , McNall-Knapp Rene , Dunn Ian F.
Published: October 2021

Introduction: H3K27M-mutated diffuse midline gliomas (H3-DMGs) are aggressive tumors with a fatal outcome. This study integrating individual patient data (IPD) from published studies aimed to investigate the prognostic impact of different genetic alterations on survival of these patients.Methods: We accessed PubMed and Web of Science to search for relevant articles. Studies were included if they have available data of follow-up and additional molecular investigation of H3-DMGs. For survival analysis, Kaplan-Meier analysis and Cox regression models were utilized, and corresponding hazard ratios (HR) and 95% confidence intervals (CI) were computed to analyze the impact of genetic events on overall survival (OS).Result: We included 30 studies with 669 H3-DMGs. TP53 mutations were the most common second alteration among these neoplasms. In univariate Cox regression model, TP53 mutation was an indicator of shortened survival (HR = 1.446; 95% CI = 1.143-1.829) whereas ACVR1 (HR = 0.712; 95% CI = 0.518-0.976) and FGFR1 mutations (HR = 0.408; 95% CI = 0.208-0.799) conferred prolonged survival. In addition, ATRX loss was also associated with a better OS (HR = 0.620; 95% CI = 0.386-0.996). Adjusted for age, gender, tumor location, and the extent of resection, the presence of TP53 mutations, the absence of ACVR1 or FGFR1 mutations remained significantly poor prognostic factors.Conclusions: We outlined the prognostic importance of additional genetic alterations in H3-DMGs and recommended that these neoplasms should be further molecularly segregated. It could help neuro-oncologists better evaluate the risk stratification of patients and consider pertinent treatments.
Effect of Soluble Amyloid Precursor Protein-alpha on Adult Hippocampal Neurogenesis in a Mouse Model of Alzheimer’s Disease
Authors: Ohline Shane M. , Chan Connie , Schoderboeck Lucia , Wicky Hollie E. , Tate Warren P. , Hughes Stephanie M. , Abraham Wickliffe
Published: October 2021

Soluble amyloid precursor protein-alpha (sAPPα) is a regulator of neuronal and memory mechanisms, while also having neurogenic and neuroprotective effects in the brain. As adult hippocampal neurogenesis is impaired in Alzheimer’s disease, we tested the hypothesis that sAPPα delivery would rescue adult hippocampal neurogenesis in an APP/PS1 mouse model of Alzheimer’s disease. An adeno-associated virus-9 (AAV9) encoding murine sAPPα was injected into the hippocampus of 8 month-old wild-type and APP/PS1 mice, and later two different thymidine analogues (XdU) were systemically injected to label adult-born cells at different time points after viral transduction. The proliferation of adult-born cells, cell survival after eight weeks, and cell differentiation into either neurons or astrocytes was studied. Proliferation was impaired in APP/PS1 mice but was restored to wild-type levels by viral expression of sAPPα. In contrast, sAPPα overexpression failed to rescue the survival of XdU+-labelled cells that was impaired in APP/PS1 mice, although it did cause a significant increase in the area density of astrocytes in the granule cell layer across both genotypes. Finally, viral expression of sAPPα reduced amyloid-beta plaque load in APP/PS1 mice in the dentate gyrus and somatosensory cortex. These data add further evidence that increased levels of sAPPα could be therapeutic for the cognitive decline in AD, in part through restoration of the proliferation of neural progenitor cells in adults.
Methanolic extract of Moringa oleifera leaves induces Cell cycle arrest and downregulates Mitochondrial membrane potential in Dalton's Lymphoma cells.
Authors: Kumar Sandeep , Shukla Alok , Verma Praveen Kumar , Singh Rishi kant , Kumar Naveen , Patel Anand kumar , Yadav Lokesh , Kumar Sanjay , Acharya Arbind
Published: October 2021

Cancer is a group of diseases characterised by abnormal and undifferentiated cell growth that has the potential to spread to other parts of the body. It is the world's second leading cause of death and morbidity. According to the GLOBOCAN 2020 report, out of 19.3 million new cancer cases and 10 million deaths reported, 544352 new cases and 259793 deaths occurred by non-Hodgkin lymphoma (NHLs). Although, numerous therapeutic approaches like, surgery, radiotherapy, chemotherapy and immunotherapy have been developed to treat cancer, limited success has been achieved, possibly due to severe side effects associated with the drugs used during chemotherapy. Therefore, deciphering the novel compound with least side effects and highly potent against cancer is urgently required. In the present study we used leaf extract of M. oleifera, well-known for its anti-cancer efficacy against different cancer cells, however, its effect on Dalton’s lymphoma, a type of spontaneously occurring T cell lymphoma originated in the thymus of DBA mice is seriously lacking. Therefore, present study was aimed to analyze the therapeutic efficacy of M. oleifera against DL cells. Our results show that leaf extract of M. oleifera (MOML) significantly induces morphological changes in DL cells followed by chromatin condensation, nuclear fragmentation, and ROS generation. We also found significant changes in mitochondrial membrane potential (ΔΨm) in a dose dependent manner. Furthermore, apoptosis of DL cells induced by cell cycle arrest at G2/M and S phase suggested that MOML could be used to treat NHL effectively
Please Let Science Talk: Presidential Sentiment and the Public’s Risk Perception in the Era of COVID-19
Authors: Guo Jie , Yang Junhong , Zeng Yeqin , Zhu Shulei
Published: October 2021

We examine whether and to what extent the presidential COVID-19 sentiment (PCS), constructed based on the former President Trump’s tweets, has influenced the public’s risk perception regarding COVID-19. We find that during the COVID-19 pandemic, PCS is negatively associated with social distancing behaviour in the Republican counties but is positively associated with social distancing behaviour in the Democratic counties. Our finding supports the view that individuals’ risk perception is influenced by the information they receive from politicians through social media, with the impact exhibiting as variations with respect to the individuals’ position on the political spectrum.
Case Report: Congenital nasal pyriform aperture stenosis with holoprosencephaly: Diagnosis and surgical management
Authors: Yilala Melcol Hailu , Dibaba Dawit Kibebew , Gebrekidan Messay , Tilahun Dessalegn
Published: October 2021

Congenital nasal pyriform aperture stenosis (CNPAS) is a rare cause of neonatal nasal obstruction that clinically mimics choanal atresia. It occurs as a result of abnormal development of primary palate and maxilla, either in isolation or in association with other anomalies. Differentiation between CNSPAS and choanal atresia is critical as management approachers are quite different from one another. Diagnosis can be made clinically and with characteristics CT findings. Management options include both conservative and surgical approaches depending on the patient’s initial condition. Here is reported a case of 33 days infant who was admitted to our NICU with a working diagnosis of CNPAS after she presented with two days of fast breathing and failure to suckle, managed surgically and discharged improved.
Anais I JASBI - I Jornada Acadêmica de Saúde Bucal Inclusiva UFPR
Authors: Mendes Pupo Yasmine , Sarot João Rodrigo , De Lima Heliton Gustavo , Lopes Jaqueline do Carmo Machado
Published: October 2021

Caro(a) Leitor(a),

A Jornada Acadêmica de Saúde Bucal Inclusiva (JASBI) é um evento científico realizado pelo projeto de extensão Saúde Bucal Inclusiva da UFPR com o objetivo proporcionar conhecimento científico diante do atendimento odontológico e multiprofissional da pessoa com deficiência, parcela muitas vezes desassistida da população, preparando os futuros e/ou profissionais da área para tratamentos de qualidade a estes pacientes. Os principais tópicos abordados no evento foram métodos minimamente invasivos de controle da doença cárie; sedação inalatória com óxido nitroso e oxigênio como opção no atendimento de pessoas com deficiência; reconhecimento do papel do fonoaudiólogo na atuação multiprofissional com o cirurgião-dentista no atendimento de pessoas com deficiência; alterações bucais presentes na cavidade bucal de pacientes idosos; percepção sobre as técnicas de manejo e importância da saúde bucal preventiva para a qualidade de vida de pessoas com deficiência; atuação do cirurgião-dentista bucomaxilofacial no tratamento de fissuras lábio palatinas. Além disso, parte do evento foi direcionado a apresentação e discussão de trabalhos científicos com mediação por professores da área de atendimento das pessoas com deficiência (PDs).

A coordenadora do projeto de extensão Saúde Bucal Inclusiva Profª. Drª. Yasmine Mendes Pupo e presidente do evento, reuniu acadêmicos de Odontologia da Universidade Federal do Paraná para compor uma comissão organizadora totalizando 22 colaboradores, internos do projeto e externos. No total o evento contou com 1200 inscritos de diversas Universidades do Brasil e 130 submissões de trabalhos científicos. O evento favoreceu a maior visibilidade das ações do projeto de extensão vinculado aumentando consideravelmente o número de seguidores no Instagram (sbi_ufpr).

A I JASBI, transmitida ao vivo pelo Youtube, aconteceu nos dias 19, 20 e 21 de maio de 2021, com temas de extrema relevância para o atendimento odontológico de PDs, o que culminou em 3700 visualizações das palestras durante a semana do evento. No dia 21, período da manhã e tarde, foram realizadas as apresentações de trabalhos selecionados de forma síncrona e os demais foram disponibilizados em playlist no Youtube de forma assíncrona. Os resumos referentes as apresentações síncronas e assíncronas encontram-se no presente anais, assim como, os trabalhos premiados no transcorrer do evento.

Comissão Organizadora

I JASBI - UFPR (Universidade Federal do Paraná)  2021
Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation
Authors: Mohd Don Siti Madihah , Abdul Hamid Nur Maziyyah , Taha Hussein , Sukri Rahayu Sukmaria , Metali Faizah
Published: October 2021

Hoya imperialis (H. imperialis) and H. coronaria (Apocynaceae) are known to have ornamental value due to their beautiful flowers; however, the feasibility of propagating these plants have not been reported despite the wild populations in Brunei Darussalam being highly threatened due to habitat loss and overcollection. Thus, the present study aimed to conduct a preliminary study of the feasibility of two alternative propagation methods, stem cutting and micropropagation, as a potential approach for their ex situ conservation. Hoya stem cuttings were treated with either indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA) (0–2000 mg/L), and then propagated onto a mixture of peat moss and perlite. For micropropagation, Hoya leaf explants were cultured onto Murashige and Skoog (MS) agar media that were supplemented with IBA and/or kinetin (KN) (0–10.0 mg/L). This present study shows that both Hoya species were successfully propagated by stem cutting even without hormone treatment. However, interestingly, in H. imperialis, when compared with control, the mean number of new leaves (6.3 ± 1.0) and the mean relative growth rate (RGR) based on stem diameter (0.004 ± 0.0007 cm cm−1 day−1) significantly increased when treated with 500 mg/L NAA and 2000 mg/L IBA, respectively. Meanwhile, in H. coronaria, significantly higher mean number of roots was achieved by treating with 1000 mg/L NAA (16.6 ± 1.4) or 2000 mg/L IBA (17.5 ± 2.7) compared with control. For micropropagation, callus induction was not promising and could only be observed at specific concentrations of both IBA and KN, with H. imperialis appearing to be more responsive towards these hormones in comparison to H. coronaria. The present study showed that stem cutting appeared more feasible in propagating both Hoya species.
Evaluation of Cellulolytic Endo-1,4-B-D-Glucanase Activity in the Digestive Fluid of Adult Phytophagous Beetle Hoplasoma unicolor
Authors: Mohammad Mosleh Uddin  , Suzana Afrin Lima  , Tanim Jabid Hossain  , Newton Kar  , Yeasmin Zahan  , Babamale AbdulKareem Olarewaju
Published: October 2021

Insects of the taxonomic order Coleoptera are recognised for considerable cellulolytic activity in their digestive fluid. The cellulolytic activity of the gut fluid in Hoplasoma unicolor, a member of Coleoptera, however, remains unexplored. In this study, we, for the first time, report the qualitative and quantitative analysis of cellulolytic activity in the digestive fluid of this insect. The cellulolytic endo-1,4-?-D-glucanase activity was confirmed in the supernatant of the insect’s digestive fluid by agar plate assay using carboxymethyl cellulose as the substrate. To determine the optimum pH, enzyme activity was further assessed in an acidic pH range of 5 to 6, and the highest activity was observed at pH 5.3. For quantitative analysis, endoglucanase activity was measured using 3,5-dinitrosalicylic acid method which revealed that the specific activity of the gut sample was 0.69 (±0.01) units per mg of protein. For further characterisation of the cellulases in the sample, SDS-PAGE and zymogram analysis were carried out. Two active cellulolytic bands were detected on the zymogram suggesting the presence of two distinct endoglucanases which completely disappeared upon heating the sample at 55°C. Our study, therefore, highlights prospect of the gut fluid of H. unicolor as an important source of cellulase enzymes that merits further investigations into their extensive characterisation for potential industrial applications.
Characterisation of Plant Growth-Promoting Endophytic Bacteria from Sugarcane and Their Antagonistic Activity against Fusarium moniliforme
Authors: Pitiwittayakul Nittaya , Wongsorn Duanpen , Tanasupawat Somboon
Published: October 2021

The use of endophytic bacteria in agriculture provides an effective way of improving crop yield and significantly reducing chemical usage, such as fungicides. This research was conducted to explore endophytic bacteria with plant growth promotion (PGP) and antifungal activities against Fusarium moniliforme AIT01. In this study, we obtained 52 isolates of endophytic bacteria associated with the roots and stems of sugarcane from Nakhon Ratchasima province, Thailand. In vitro antagonistic activity test showed that 14 out of 52 isolates had antagonistic activity against the fungal pathogen F. moniliforme AIT01. These antagonistic endophytic bacteria were identified as belonging to six different species as follows: Nguyenibacter vanlangensis, Acidomonas methanolica, Asaia bogorensis, Tanticharoenia aidae, Burkholderia gladioli and Bacillus altitudinis based on phenotypic characteristics, along with phylogenetic analysis of their 16S rRNA gene sequences. Seven isolates effectively inhibited F. moniliforme AIT01 mycelial growth by up to 40%. The volatile compounds of six isolates reduced the growth of F. moniliforme AIT01 by over 23%. Moreover, riceberry rice seedlings previously treated with B. gladioli CP28 were found to strongly reduce infection with phytopathogen by 80% in comparison to the non-treated control. Furthermore, the isolates also showed relevant PGP features, including ammonia production, zinc and phosphate solubilisation, auxin and siderophore biosynthesis. These results demonstrated that the tested endophytic bacteria could be successfully utilised as a source of PGP and biocontrol agent to manage diseases caused by F. moniliforme.
Nest Characteristics of the Sumatran Orangutan (Pongo abelii) in the Wildlife Sanctuary Soraya Station in Aceh Province, Indonesia
Authors: Andini Rita , Rahmi Erdiansyah , - Mardiana , Rasnovi Saida , - Martunis , Moulana Ryan
Published: October 2021

Orangutans (Pongo spp.) populations used to be widely distributed throughout Southeast Asia, from Java in the south to the Southern China in the north during the Pleistocene. Their populations have declined up to 75% of their original size and are now distributed only in parts of the tropical rainforests of Borneo and Sumatra. Pongo pygmaeus, Pongo tapanuliensis and Pongo abelii are the three most representative species, in this study, here we discussed the latter. Sumatran forests are generally suffering from deforestation with rates ranging from 3.74% to 49.85% between 2000 and 2012. Thus, human wildlife conflict intensity has escalated and gained more traction. Orangutans are known as arboreal great apes and need to build nests for resting. We applied the transect line method (three transects; each 1,000 m long) at different elevations in Soraya Research Station, Gelombang Village, Sultan Daulat sub-district, Subulussalam district, and assessed the nest characteristics. The characteristics are: (1) nesting position referring to the position of nest on a tree; (2) nest successional stages defining the age and leaf decay used in constructing a nest indicated with I (new) until V (almost gone); and (3) nest density to predict the density of nest per square km. Afterwards, the identified nesting trees along the transect were further identified based on their species, and assessed based on three characteristics (the tree height, diameter and the height of a nest measured from the soil). A total of 27 nests were found, and 44% were located in transect III or at the riparian. Out of 27, four orangutans’ nests were found on Moraceae (Streblus elongatus) and Myrtaceae (Syzigium spp.), while three nests were found on Dipterocarpus sp. The tree height, tree diameter and nest height were 10 m–25 m (mean = 17.5 m; SD = ± 0.25), 10 cm–30 cm (mean = 20 cm; SD = ± 0.4) and 16 m–20 m (mean = 18 m; SD = ± 0.35), respectively. Meanwhile, nest density calculated based on the form: d = [N/ (L* 2w)], and the values obtained were 8.4, 13.45, 26.9 nests/km2 located on transect I, II and III or at the riparian. The most commonly found nest successional stages and position were stage III and position 3, respectively. This study could serve as a baseline research in primate conservation and nest characterisation could be used as guidance for any future activity planning (e.g. tree reforestation) in a particular region and the existence of various tree species diversity are indispensable for maintaining orangutan habitats’ quality.
The hidden WASH needs of perimenopausal women
Authors: Bhakta Amita , Esseku Harold , Esseku Yvonne , Fisher Julie , Reed Brian
Published: October 2021

Meeting the needs of an ageing population, particularly in the global South, is increasingly an issue for WASH service provision. An ageing-related issue for women, warranting specific attention from the WASH sector, is the perimenopause, but this is often not discussed publicly. Drawing on data from the UK and Ghana, this paper provides recommendations for meeting the additional WASH needs of women as they experience the perimenopause (the transition period to menopause). Finding these ‘hidden’ needs involved a UK-based phenomenological review and mixed feminist methods in two low-income urban communities in Accra and Kumasi, Ghana. The hidden WASH needs of perimenopausal women included understanding the perimenopause, menstrual hygiene management, urine incontinence management, bathing, and laundry. Community awareness, engagement and empowerment, and ensuring good perimenopausal health can begin to address these needs. Infrastructural measures for perimenopausal women include a continuous water supply, user-friendly bathing and laundry infrastructure, and gender-sensitive, accessible toilets with discrete sanitary disposal bins. High-absorbency sanitary products are important for managing heavy menstruation. A better understanding of the needs of perimenopausal women and training on how to meet these would benefit the WASH sector in ensuring that perimenopausal women are not left behind in efforts to meet the Sustainable Development Goals.
Solar homogeneous catalysis to the removal of organic matter from slaughterhouse effluents undergone to a prior biological process
Authors: Hurtado J. A. , Valdez L. F. , Escudero C. J.
Published: October 2021

This study shows the effectiveness of the wastewater treatment from a municipal slaughterhouse that has undergone a previous biological treatment applying a sequence of stages, reaching a 75% of elimination of the chemical oxygen demand (COD) using sedimentation in combination with coagulation–flocculation, using 0.5 g/L FeCl3 which is one of the best known inorganic coagulants. Then, the elimination of COD was around 98% adding the Fenton process in which 1,000 mg/L H2O2 and FeSO4 were used. In addition to the COD, other water quality parameters were measured to evaluate the level of purification of the test samples, such as solids of different types, pH, DOC and so on. With the above process, it can be noted that the Fenton process produced a slight improvement in the effluent quality by using a solar concentrator in the now-called photo-Fenton process, reaching around 99% of COD removal (0.36 g/L), 91% of total suspended solids (0.32 g/L) and 89% of dissolved organic carbon (0.20 g/L). These results were the best achieved within a proposed treatment train for this type of complex wastewater. Moreover, this last part of the process adds an improvement by the usage of renewable energy sources such as sunlight.
Stress Testing the Impact of Esg-Factors on Companies in the Metals Industry
Authors: Emelyanova E. S. , Vasiliev L. S.
Published: October 2021

Stress testing as an effective method of risk management and crisis management can serve as an effective tool for predicting financial stability, particularly under the impact of ESG risks for companies in various industries working on the subjects’ territory of the Russian Federation. The article considers an approach to stress testing algorithm development for metals industry. At the first stage the current state of the metals industry and trends of further development observed by analysts are considered, the largest domestic representatives of metallurgy are analyzed and their key targets and costs of environmental projects are highlighted. Further, industry risk drivers for organizations doing business in the metals industry are presented. After identifying risk drivers, an approach to assessment of the impact of identified ESG risks is presented, then an algorithm for assessing the impact of ESG risks on the cash flow of the Russian metals industry company is formulated and a scheme of ESG stress testing for a metallurgical company is presented. The conclusion of the study identifies various mathematical models that can be used to improve the forecast quality of the impact of ESG factors, gives the key challenges to be resolved for the development of ESG risk management and the improvement of ESG culture in the business community as a whole, and summarizes the benefits of ESG stress testing, which can be used for a whole list of different managerial and methodological actions.
Obecność eucharystyczna a uobecnienie Chrystusa w słowie Bożym. Studium inspirowane poglądami Roberta Sokolowskiego
Authors: Zatwardnicki Sławomir
Published: October 2021

Robert Sokolowski pisał, że biblijne słowa uaktywniają obecność Boga. Czy można jednak mówić o sakramentalności słowa analogicznej do samego sakramentu? W artykule zebrano refleksje na temat „uobecnienia” słowa Bożego intra mysteriorum celebrationem. Kwestia ta wiąże się z tematem związku przeszłości z teraźniejszością w Eucharystii, którym zajmował się Sokolowski. W artykule zrekapitulowano poglądy filozofa dotyczące obecności eucharystycznej, w dialogu z którym dokonano następnie namysłu nad obecnością Chrystusa w słowie Bożym. Z „paschalnocentryczności” Pisma Świętego wynika jakiegoś rodzaju uobecnienie antycypacyjnego wydania się Boga w wydarzeniach zaświadczonych w natchnionych tekstach. Liturgia słowa musi prowadzić do kulminacyjnego wydarzenia Paschy i jego sakramentalnego uobecnienia. Nie może być mowy o „dwóch obecnościach” Pana w czasie Mszy Świętej. Słowa ustanowienia z Ostatniej Wieczerzy pełnią rolę w aktualizacji Paschy, zaś natchnione słowa nie są w taki sam sposób słowami „ustanawiającymi”. Dopiero w ich wewnętrznym ukierunkowaniu prowadzą do misterium Chrystusa obecnego w Najświętszym Sakramencie.
Copaiba oil-resin (Copaifera langsdorfii Desf.: Caesalpiniaceae) associated with laser therapy for skin wound treatment in Wistar rats
Authors: Barquete Carolina Couto , Oliveira Romulo Silva de , Neves Eduardo Cavalcante das , Pelizzari Charles , Santos Joelma de Faria , Souza Rodrigo Gomes de , Caleffo Tatiane , Viott Aline de Marco , Laskoski Luciane Maria , Souza Soraia Figueiredo de
Published: October 2021

Alternative protocols for the treatment of skin lesions have been developed with the use of techniques such as photobiomodulation and phytotherapy, aiming to optimize this process. To evaluate the effectiveness of copaiba (Copaiferalangsdorffii) oil-resin and low-level laser therapy for treating cutaneous wounds, 15 Wistar rats (Rattusnorvergicus) were used, in whom five 8-mm lesions were produced. The following protocols were applied: negative control group (T1); positive control group (T2); laser therapy with AsGa (904 nm), continuous, focal mode for 10 s, dosage of 4 J/cm² (T3); copaiba oil-resin (T4); and association group (copaiba and low-level laser) (T5). The efficacy of each technique was evaluated based on macroscopic aspects of the lesion, wound healing rate, and histopathological analysis (inflammatory infiltrate and collagen expression). The Kruskal-Wallis test was used for statistical analyses (P> 0.05). Copaiba treatment showed an advantage in type III collagen expression, whereas laser therapy demonstrated an enhanced capacity for tissue regeneration. The significant advantage obtained from the association treatment is the improvement of the macroscopic aspect of the wound, with a reduction in crust formation.
Bovine rabies in Rio Grande do Sul: positivity rates, geographical distribution, and seasonal occurrence
Authors: Cappelari Bruno Egídio , Silveira Jéssica Grace da , Rosa Julio César de Almeida , Ferreira José Carlos , Dantas Giovana
Published: October 2021

Rabies is a zoonotic disease characterized by acute viral encephalitis. It is almost 100% fatal for infected animals. While cases of human rabies have still been registered in Brazil, in the state of Rio Grande do Sul rabies is considered a controlled disease in urban areas. However, the transmission of bovine rabies – which has been largely associated with the Desmodus rotundus bat – is endemic. Additionally, there are estimates that only 10% of rabies cases are reported. Therefore, this study aimed to describe the positivity rates, geographical distribution, and seasonal occurrence of bovine rabies in RS, as well as the sampling practices in rabies surveillance. Using samples submitted for laboratory diagnosis from 2016 to 2019, we conducted a retrospective study of the sample results, positivity rates, and seasonal occurrence of rabies in cattle. Throughout these four years, less than a third of state municipalities sent samples for diagnosis, suggesting that the occurrence of rabies might still be underestimated in non-sampled areas. A higher number of rabies cases were reported in 2019, a year in which the most diagnosed samples and the highest positivity percentages were recorded. However, the case numbers registered between 2016 and 2019 were not significantly different. We found that the summer and autumn months presented statistically different positivity rates. Besides this, we did not find any positive D. rotundus samples, despite the positivity rates for bovine rabies. Active surveillance, especially of bat populations and under-sampled regions, must be strengthened to correctly estimate the impact of rabies.
Renal profile and urinary fractional excretion of calcium, phosphorus, and magnesium of lambs fed with different contents of phosphorus
Authors: Santarosa Bianca Paola , Branchini Naiana da Silva , Ferreira Danilo Otávio Laurenti , Surian Soraya Regina Sacco , Dabus Daniela Marques Maciel , Hooper Henrique Barbosa , Coelho Matheus Ribeiro , Fioratti Eduardo Gorzoni , Lopes Raimundo Souza , Gonçalves Roberto Calderon
Published: October 2021

Obstructive urolithiasis is common in confined sheep, and a predominant factor predisposing animals to this condition is nutritional management. The objective of this study was to evaluate the effect of different concentrations of phosphorus (P) on urinalysis, serum urea and creatinine, and serum and urine concentrations of calcium (Ca), P, and magnesium (Mg), as well as to establish the fractional excretion (FE) of these elements in confined lambs. Thirty male Santa Inês x Ile de France sheep at four months of age were divided into two groups according to the diet: Group 1 (G1, n = 15), with 0.43% P in their feed; and Group 2 (G2, n = 15) with 0.65% P. The diets consisting of 10% Coast-cross hay and 90% of a concentrate of soybean meal, wheat, and crushed corn were provided for 90 consecutive days, with water ad libitum. The time points (M) of blood and urine collection were determined as M0, immediately before the beginning of the diet, and at 15-day intervals: M1, M2, M3, M4, M5, and M6. Phosphorous-rich diets favored the appearance of triple phosphate crystals in both groups, causing calculogenesis in more than one third of the animals (11/30), emphasizing the relevance of conducting laboratory tests to aid in urolithiasis diagnosis. In addition, FE revealed an increased renal excretion of excess minerals in the diet, and therefore a greater predisposition for stone formation in relation to nutritional management.
The Male Reproductive System and Endocrine Disruptors
Authors: Schiesaro Mauro Giovanni , Amato Anna Maria Letizia , Maneschi Chiara , Sciabica Vincenzo , Pigatto Erika , Sanna Marta
Published: October 2021

:

The male reproductive system is exposed to a great number of chemical substances which can interfere with the normal hormonal milieu and reproductive function; these are called endocrine disruptors (EDs). Despite a growing number of studies evaluating the negative effects of EDs, their production is continuously growing although some of them have been prohibited. The prevalence of poor semen quality, hypospadias, cryptorchidism, and testicular cancer has increased in the last dec-ades, and recently, it has been postulated that these could all be part of a unique syndrome called tes-ticular dysgenesis syndrome. This syndrome could be related to exposure to a number of EDs which cause imbalances in the hormonal milieu and oestrogenic over-exposure during the foetal stage. The same EDs can also impair spermatogenesis in offspring and have epigenetic effects. Although stud-ies on animal and in vitro models have raised concerns, data are conflicting. However, these studies must be considered as the basis for future research to promote male reproductive health.
Copper and Zinc Co-doped Titanium Dioxide Nanotubes Arrays on Controlling
Nitric Oxide Releasing and Regulating the Inflammatory Responses
for Cardiovascular Biomaterials
Authors: Yang Ping , Yin Benli , Luo Xiao , Gao Peng Yu , Jiao Zhi Sha , Piao Jun Ji , Zhao An Sha
Published: October 2021

Background:

Titanium dioxide (TiO2) nanotubes arrays have shown tremendous application
foreground due to their unique characters of structure and performance. However, the single
bio-function is still the limit on cardiovascular biomaterials.

Methods:

The loadability function provides the possibility for the TiO2 nanotubes arrays to realize
composite multifunction. The copper can catalyze the release of nitric oxide to promote the proliferation
of endothelium cells and improve the anticoagulant. Also, zinc can adjust the inflammatory responses
to improve anti-inflammation.

Results:

In this work, we co-doped the copper and zinc onto TiO2 nanotubes arrays to estimate the
hemocompatibility, cytocompatibility and responses of inflammation. The results showed that copper
and zinc could introduce better multi-biofunctions to the TiO2 nanotubes arrays for the application
in cardiovascular biomaterials.

Conclusion:

In summary, the NTs@Cu/Zn sample as a new composite material in this study had
significant biocompatibility in vascular implantation and can be used as a potential material for polymer-
free drug-eluting stents.
Chemotherapy Resistance in Remutation of Epidermal Growth Factor Receptor Wild Type Becomes a Positive Type and Back Becomes a Wild Type in A Patient with Lung Adenocarcinoma
Authors: Kurniawan Kristo , Sugiri Yani Jane , Parsama Putra Ngakan Putu , Yudhanto Handy Setyo
Published: October 2021

Introduction: Lung cancer is the most common type of cancer worldwide (11.6%) and the leading cause of death due to cancer throughout the world. One type of lung cancer that is often found is Adenocarcinoma, 35-40%. Mutations in EGFR often occur in patients with pulmonary Adenocarcinoma, especially in Asia. Chemotherapy selection for pulmonary adenocarcinoma patients based on the status of their EGFR mutations. Positive EGFR mutations can get treatment with Tyrosine Kinase Inhibitors. Giving chemotherapy can affect changes in EGFR mutation status. Patients with chemotherapy treatment can experience resistance to chemotherapy either primary or acquired resistance through a variety of mechanisms.Case Description: we reported one case of a 56-year-old man with pulmonary adenocarcinoma who had a positive change in EGFR-type from wild type mutations and then returned to a wild type. Patients were initially diagnosed with wild-type pulmonary adenocarcinoma from EGFR examination of tissue biopsy and given conventional chemotherapy. During the evaluation, progression occurred so that the status of the EGFR mutation was examined using ct-DNA and the result was mutation deletion exon 19 so that the patient obtained Gefitinib. Due to progressive return, the patient again examined EGFR status from tissue biopsy obtained using pleuroscopy and obtained an EGFR wild type. Patients again get conventional chemotherapy.Discussion Changes in the status of EGFR mutation in pulmonary adenocarcinoma patients and chemotherapy resistance can occur in patients with chemotherapy treatment.Â
QUALITY OF LIFE FOR CHILDREN WITH GROWTH HORMONE DEFICIENCY: SIGNIFICANCE OF CLINICAL, PSYCHOEMOTIONAL AND SOCIO-DEMOGRAPHIC FACTORS
Authors: Aryayev Mykola , Senkivska Liudmyla
Published: October 2021

The work is devoted to assessing the quality of life (QоL) of children with growth hormone deficiency (GHD)  and  determining the correlation between the QoL and clinical, psycho-emotional and socio-demographic factors. A prospective observational cohort study was carried out at the «Odessa Regional Children's Clinical Hospital» among 46 prepubertal children with GHD and 80 healthy children matched for sex and age. The QоL was determined using Peds QL4.0 questionnaires, which were completed by children and their parents. Total psycho-emotional problems, internalizing and externalizing problems were investigated using 2-factor model of the «Strengths and Difficulties Questionaire (SDQ)», which was completed by the child's parents. The indices of the Peds QL4.0 and SDQ scale were fixed at baseline (T0) and after 12 months (T12) therapy with recombinant human growth hormone (rHGH) at an average dose of 0.033 mg/kg/day. The difference between the observed means ± SD in two independent samples were assessed using t-tests and «p» values. The relationship between clinical, psychological, socio-demographic factors and QoL of children with GHD was assessed by the calculation of Spearman’s correlation coefficient (rs).It was found that GHD was the cause for the decrease in the QoLaccording to the «total score» of the Peds QL4.0 scale for children and acccording to the subscales «physical health», «psychosocial health», «emotional functioning», «social functioning», «school functioning». The overall assessment in the SDQ «total difficulties» score increased and the conceptualization of internalizing problems occurred in children with GHD. The 12-months rHGH therapy contributes to the improvement of QoL in children with GHD, the normalization of the psycho-emotional state and the decrease in SDQ «total difficulties» and in «internalizing problems» scores.
INFLUENCE OF TYPE 2 DIABETES MELLITUS ON THE PREVENTION AND TREATMENT OF PROSTHETIC STOMATITIS
Authors: Badalov Robert , Chernyavskaya Irina
Published: October 2021

Purpose: to study the effect of type 2 diabetes mellitus on the state of the mucous membrane and tissues of the oral cavity to optimize the prevention and increase the effectiveness of dentition defects complex treatment in this contingent of patients. Materials and methods. We examined 112 patients with type 2 diabetes mellitus aged 35 to 64 years, who were further divided into three groups. The state of the oral mucosa, morphological changes, local immunity, lysozyme were determined. The work used morphological, microscopic methods, enzyme immunoassay and spectrophotometry. To test the statistical significance of intergroup differences, the nonparametric Mann- Whitney test was used. Results. Oral use in patients with diabetes mellitus type 2, applications of 1% Clotrimazole cream and Echinacea Compositum S had a positive preventive and therapeutic effect, which was accompanied by an increase in capillary resistance, a decrease in the degree of migration of leukocytes, an increase in the functional activity of immune system cells and an increase in the level of regenerative processes. In patients with defects in the dentition against the background of type 2 diabetes mellitus, violations of the oral cavity local immunity were revealed, characterized by a significant increase in the S-IgA content and a decrease in lysozyme.Orthopedic treatment with partial removable denturesled to a significant decrease in the concentration of S-IgA and an increase in the bactericidal activity of saliva due to increased lysozyme synthesis. It was concluded that type 2 diabetes mellitus affects the state of the oral mucosa, which must be taken into account when providing orthopedic and dental treatment.
THE ROLE OF HYPERLEPTINEMIA IN THE GENESIS OF GESTATIONAL HYPERTENSION AND PREECLAMPSIA
Authors: Ostafiichuk Svitlana , Drohomyretska Nataliia , Motsyuk Yuliia , Neіko Olha , Henyk Natalia
Published: October 2021

The aim of the study was to determine the association of serum leptin levels in pregnant women with the risk of hypertensive disorders depending on gestational weight gain (GWG). We have studied 190 pregnant women with a normal pre-pregnancy body mass index, who were registered at the City Clinical Perinatal Center, Ivano-Frankivsk, Ukraine. Quantitative determination of leptin in serum was performed by ELISA Kit (CAN-L-4260, Canada). Statistical analyses based on Microsoft Excel statistical analysis package and Statistica 6.0. Gestational hypertension (GH) has developed in 29 (15.3 %), preeclampsia in 39 (20.5 %) cases and 122 (64.2%) pregnant were normotensive. The recommended GWG (11.5-16.0 kg) was diagnosed in 133 (70.0 %), excessive in 57 (30.0 %) patients. The frequency of GH was 2.2-fold (p<0.05) and preeclampsia was 4.2-fold (p<0.001) higher in pregnant with excessive GWG, compared to patients with the recommended GWG. Leptin concentrations in pregnant with GH were 1.5-fold in the third trimester (p<0.01), and with preeclampsia 1.1-fold in the second (p<0.01) and 1.5-fold in the third (p<0.01) trimesters higher compared to normotensive women. Serum leptin concentrations of pregnant with GH and preeclampsia were significantly higher in the group with excessive GWG compared to the recommended, which were detected in the second trimester. A high degree of risk of hyperleptinemia in the groups of pregnant with GH (OR=3.50; 95%CI: 1.48-9.33; p<0.05), and with preeclampsia (6.01; 2.43-15.02) was found in women with excessive GWG. A positive correlation was found between leptin concentration, GWG and body fat percentage in pregnant with GH and preeclampsia in the second and third trimesters. Thus, leptin can be considered a marker of the association between pathological GWG and hypertensive disorders in pregnancy
FEATURES OF MANAGEMENT IN PATIENTS WITH OSTEOPOROSIS AND DIABETES MELLITUS
Authors: Zhuravlyova Larysa , Oliinyk Mariia , Sikalo Yulia
Published: October 2021

The number of patients with diabetes mellitus in the world has been progressively increasing in recent years, therefore, the fight against complications of diabetes mellitus is an important problem of our time. The purpose of our review is to analyze the literature data on the risks of osteoporosis in patients with diabetes mellitus, effective diagnostic methods, as well as current recommendations for the treatment and prevention of osteoporosis in this category of patients. Results. We have processed and analyzed literary sources and internationalrecommendations, which identify the main mechanisms and risk factors that contribute to the development of osteoporosis in patients with type 1 and 2 diabetes mellitus; methods for the timely diagnosis of osteoporosis are indicated, methods for correcting the condition of patients with diabetes mellitus, which can help prevent the development of osteoporosis in this group of patients, are given. The current recommendations for the treatment of osteoporosis in men and women are presented. Conclusions. Taking into account the data of the analysis of literary sources, osteoporosis can be considered one of the complications of diabetes mellitus. Today, densitometry and fracture risk assessment FRAX are quite sensitive methods for early diagnosis of osteoporosis in patients with type 1 diabetes mellitus, but not sufficient for patients with type 2 diabetes mellitus, therefore there is a need to determine the trabecular bone index during densitometry, as well as additional actions when assessing the risk of fractures on the FRAX scale. Therapy of patients with osteoporosis with concomitant diabetes mellitus should be based on the achievement of target glycemic levels and the use of bisphosphonates with target level of calcium and vitamin D.
Factors Influencing the Intention of Actors in Hospitals to Use Indoor Positioning Systems: Reasoned Action Approach (Preprint)
Authors: Wichmann Johannes , Leyer Michael
Published: October 2021

BACKGROUND

Indoor positioning systems (IPS) have become increasingly important for several branches of the economy (eg, in shopping malls) but are relatively new to hospitals and underinvestigated in that context. This research analyzes the intention of actors within a hospital to use an IPS to address this gap.

OBJECTIVE

To investigate the intentions of hospital visitors and employees (as the main actors in a hospital) to use an IPS in a hospital.

METHODS

The reasoned action approach was used, according to which the behavior of an individual is caused by behavioral intentions that are affected by (1) a persuasion that represents the individual’s attitude toward the behavior, (2) perceived norms that describe the influence of other individuals, and (3) perceived norms that reflect the possibility of the individual influencing the behavior.

RESULTS

The survey responses of 323 hospital visitors and 304 hospital employees were examined separately using SmartPLS 3.3.3. Bootstrapping procedures with 5000 subsamples were used to test the models (one-tailed test with a significance level of .05). The results show that attitude (β=.536; P<.001; f²=.381) and perceived norms (β=.236; P<.001; f²=.087) are predictors of hospital visitors’ intention to use an IPS. In addition, attitude (β=.283; P<.001; f²=.114), perceived norms (β=.301; P<.001; f²=.126), and perceived behavioral control (β=.178; P=.005; f²=.062) are predictors of hospital employees’ intention to use an IPS.

CONCLUSIONS

This study has two major implications: (1) our extended reasoned action approach model, which takes into account spatial abilities and personal innovativeness, is appropriate for determining hospital visitors’ and employees’ intention to use an IPS; and (2) hospitals should invest in implementing IPS with a focus on (a) navigational services for hospital visitors and (b) asset tracking for hospital employees.
Empowerment of Watermelon Farmer at Mojosari Village Puger District Jember in Producing Watermelon Low Pesticide Residue
Authors: Haryadi Nanang Tri , Purnomo Hari , Agusina Titin
Published: October 2021

Mojosari Village, Puger Sub-district, is the largest watermelon center in Jember Regency. In 2017, the total harvested area reached 900 Ha and productivity reached 290.72 kW / Ha. The problem that often arises in watermelon cultivation is the attack of plant pests. Pests that often attack watermelons include caterpillars, whitefly, fruit flies, and thrips. Pest control that is often used by farmers is sprayed with chemical pesticides. The spraying of chemical pesticides began to be complained about by farmers because they were not able to control pests, this was probably because the pests were already resistant to pesticides. The decline in watermelon quality is also caused by soil conditions that have begun to decrease in nutrient content, due to the use of excessive chemical fertilizers. Farmers become dependent on chemical pesticides and chemical fertilizers in watermelon cultivation. Therefore, it is necessary to educate farmers to reduce the use of excessive chemical pesticides and fertilizers. One alternative is to use environmentally friendly biological control agents. The result of this activity is that farmers can reproduce biological agents, namely the fungus Metarhizium anisopliae, Beauveria bassiana, Trichoderma, entomopathogenic nematodes, and make organic fertilizers. Farmers can produce biological agents and apply them to watermelon fields. The resulting watermelon is more durable to store and does not rot easily after harvest.