bluuebunny/crossref_metadata_2025 huggingface dataset:
Zirconium-Based Metal Organic Frameworks for the Capture of Carbon Dioxide and Ethanol Vapour. A Comparative Study
Authors: Saidi Meryem , Ho Phuoc Hoang , Yadav Pankaj , Salles Fabrice , Charnay Clarence , Girard Luc , Boukli-Hacene Leila , Trens Philippe
Published: December 2021

This paper reports on the comparison of three zirconium-based metal organic frameworks (MOFs) for the capture of carbon dioxide and ethanol vapour at ambient conditions. In terms of efficiency, two parameters were evaluated by experimental and modeling means, namely the nature of the ligands and the size of the cavities. We demonstrated that amongst three Zr-based MOFs, MIP-202 has the highest affinity for CO2 (−50 kJ·mol−1 at low coverage against around −20 kJ·mol−1 for MOF-801 and Muc Zr MOF), which could be related to the presence of amino functions borne by its aspartic acid ligands as well as the presence of extra-framework anions. On the other side, regardless of the ligand size, these three materials were able to adsorb similar amounts of carbon dioxide at 1 atm (between 2 and 2.5 µmol·m−2 at 298 K). These experimental findings were consistent with modeling studies, despite chemisorption effects, which could not be taken into consideration by classical Monte Carlo simulations. Ethanol adsorption confirmed these results, higher enthalpies being found at low coverage for the three materials because of stronger van der Waals interactions. Two distinct sorption processes were proposed in the case of MIP-202 to explain the shape of the enthalpic profiles.
SiO2 Coated Up-Conversion Nanomaterial Doped with Ag Nanoparticles for Micro-CT Imaging
Authors: Zhang Wei , Lu Yanli , Zang Yang , Han Jinhui , Xiong Qingyun , Xiong Jinping
Published: December 2021

In this study, a new method for synthesizing Ag-NaYF4:Yb3+/Er3+ @ SiO2 nanocomposites was introduced. Using a hydrothermal method, the synthesized Yb3+- and Er3+-codoped NaYF4 up-conversion luminescent materials and Ag nanoparticles were doped into up-conversion nanomaterials and coated with SiO2 up-conversion nanomaterials. This material is known as Ag-UCNPs@SiO2, it improves both the luminous intensity because of the doped Ag nanoparticles and has low cytotoxicity because of the SiO2 coating. The morphology of UCNPs was observed using scanning electron microscopy (SEM), and the mapping confirmed the successful doping of Ag nanoparticles. Successful coating of SiO2 was confirmed using transmission electron microscopy (TEM). Fluorescence spectra were used to compare changes in luminescence intensity before and after doping Ag nanoparticles. The reason for the increase in luminescence intensity after doping with Ag nanoparticles was simulated using first-principles calculations. The cytotoxicity of Ag-UCNPs@SiO2 was tested via the cell counting kit-8 (CCK-8) method, and its imaging ability was characterized using the micro-CT method.
Modelling of Dynamic Behaviour in Magnetic Nanoparticles
Authors: Rietberg Max Tigo , Waanders Sebastiaan , Horstman-van de Loosdrecht Melissa Mathilde , Wildeboer Rogier R. , ten Haken Bennie , Alic Lejla
Published: December 2021

The efficient development and utilisation of magnetic nanoparticles (MNPs) for applications in enhanced biosensing relies on the use of magnetisation dynamics, which are primarily governed by the time-dependent motion of the magnetisation due to externally applied magnetic fields. An accurate description of the physics involved is complex and not yet fully understood, especially in the frequency range where Néel and Brownian relaxation processes compete. However, even though it is well known that non-zero, non-static local fields significantly influence these magnetisation dynamics, the modelling of magnetic dynamics for MNPs often uses zero-field dynamics or a static Langevin approach. In this paper, we developed an approximation to model and evaluate its performance for MNPs exposed to a magnetic field with varying amplitude and frequency. This model was initially developed to predict superparamagnetic nanoparticle behaviour in differential magnetometry applications but it can also be applied to similar techniques such as magnetic particle imaging and frequency mixing. Our model was based upon the Fokker–Planck equations for the two relaxation mechanisms. The equations were solved through numerical approximation and they were then combined, while taking into account the particle size distribution and the respective anisotropy distribution. Our model was evaluated for Synomag®-D70, Synomag®-D50 and SHP-15, which resulted in an overall good agreement between measurement and simulation.
Lithium Diffusion in Silicon Encapsulated with Graphene
Authors: Qin Wei , Lu Wen-Cai , Xue Xu-Yan , Ho Kai-Ming , Wang Cai-Zhuang
Published: December 2021

The model of a graphene (Gr) sheet putting on a silicon (Si) substrate is used to simulate the structures of Si microparticles wrapped up in a graphene cage, which may be the anode of lithium-ion batteries (LIBS) to improve the high-volume expansion of Si anode materials. The common low-energy defective graphene (d–Gr) structures of DV5–8–5, DV555–777 and SV are studied and compared with perfect graphene (p–Gr). First-principles calculations are performed to confirm the stable structures before and after Li penetrating through the Gr sheet or graphene/Si-substrate (Gr/Si) slab. The climbing image nudged elastic band (CI-NEB) method is performed to evaluate the diffusion barrier and seek the saddle point. The calculation results reveal that the d–Gr greatly reduces the energy barriers for Li diffusion in Gr or Gr/Si. The energy stability, structural configuration, bond length between the atoms and layer distances of these structures are also discussed in detail.
Hybrid Sol–Gel Matrices Doped with Colorimetric/Fluorimetric Imidazole Derivatives
Authors: Sousa Rui P. C. L. , Figueira Rita B. , Gomes Bárbara R. , Sousa Sara , Ferreira R. Cristina M. , Costa Susana P. G. , Raposo M. Manuela M.
Published: December 2021

Organic–inorganic hybrids (OIH) are materials that can be easily synthesized by the sol–gel method and combine the advantages of organic and inorganic moieties within a single polymeric matrix. Imidazole derivatives are versatile organic compounds that can change their optical properties with the variation of pH due to the protonation or deprotonation of the nitrogen atoms. This work reports the preparation of different OIHs doped with different contents of two imidazole compounds (3a,b). The obtained materials were characterized structurally by FTIR, and the dielectric properties were studied by electrochemical impedance spectroscopy. The optical properties were studied by UV-Vis absorption and fluorescence spectroscopies. The FTIR analysis showed that the presence of the imidazole does not change the structural properties of the matrices. The normalized resistance values obtained for the doped matrices ranged between 8.57 and 9.32 Ω cm2, all being higher than the undoped matrix. The σ ranged between 9.49 and 10.28 S cm−1, being all higher than the pure OIH samples. Compound 3a showed a maximum absorption peak at 390 nm, which is present in the OIH spectra, proving the presence of the compound. In the case of compound 3b, a maximum absorption wavelength at 412 nm was found, and the compound peak was not clear, which may indicate that an interaction between the compound and the matrix occurred. A synergetic effect between the intrinsic emission of the matrix and the fluorescence of 3a is found on the OIH-doped matrices.
Magnetic Nanomaterials for Arterial Embolization and Hyperthermia of Parenchymal Organs Tumors: A Review
Authors: Kazantseva Natalia E. , Smolkova Ilona S. , Babayan Vladimir , Vilčáková Jarmila , Smolka Petr , Saha Petr
Published: December 2021

Magnetic hyperthermia (MH), proposed by R. K. Gilchrist in the middle of the last century as local hyperthermia, has nowadays become a recognized method for minimally invasive treatment of oncological diseases in combination with chemotherapy (ChT) and radiotherapy (RT). One type of MH is arterial embolization hyperthermia (AEH), intended for the presurgical treatment of primary inoperable and metastasized solid tumors of parenchymal organs. This method is based on hyperthermia after transcatheter arterial embolization of the tumor’s vascular system with a mixture of magnetic particles and embolic agents. An important advantage of AEH lies in the double effect of embolotherapy, which blocks blood flow in the tumor, and MH, which eradicates cancer cells. Consequently, only the tumor undergoes thermal destruction. This review introduces the progress in the development of polymeric magnetic materials for application in AEH.
Quality of Nutrition Environments, Menus and Foods Served, and Food Program Achievement in Oklahoma Family Child Care Homes
Authors: Williams Bethany D. , Sisson Susan B. , Stinner Emily L. , Hetrick Hope N. , Dunlap Marny , Graef-Downard Jennifer , Eliot Kathrin , Finnell Karla , Salvatore Alicia L.
Published: December 2021

Child care environments foster children’s healthy eating habits by providing exposure to healthy foods and feeding practices. We assessed the healthfulness of nutrition environments, menu/meal quality, and the achievement of Child and Adult Care Food Program (CACFP) guidelines and best practices in Oklahoma CACFP-enrolled family child care homes (FCCHs) (n = 51). Two-day classroom observations were conducted. Healthfulness of classroom nutrition environments was assessed using the Environment and Policy Assessment and Observation (EPAO). Foods served to and consumed by children were quantified using the Dietary Observations in Child Care (DOCC) tool. Nutrient analysis was performed to determine total energy for foods listed on menus, served to, and consumed by children. Menu and meal food variety and CACFP Guideline Achievement Scores were determined. Average nutrition environment score was 11.7 ± 1.2 (61.5% of maximum possible score). Energy (kcals) from menus and consumed by children was insufficient to meet two-thirds of their daily reference intake. Children were exposed to 1.7 vegetables and 1.3 fruits per meal. CACFP Guideline Achievement Scores were 66.3% ± 7.8 for menus and 59.3% ± 7.6 for mealtimes. Similar to previous research, our findings indicate a need for improved FCCH nutrition practices. Tailored interventions for FCCHs are needed.
Associations between Avocado Consumption and Diet Quality, Dietary Intake, Measures of Obesity and Body Composition in Adolescents: The Teen Food and Development Study
Authors: Segovia-Siapco Gina , Paalani Michael , Oda Keiji , Pribis Peter , Sabaté Joan
Published: December 2021

Avocado is a nutrient-rich food that has been shown to benefit the health and diet quality of adults. In this paper, we examined if habitual intake of avocado among adolescents is associated with their diet quality, food and nutrient intake, and measures of obesity and body composition. Participants aged 12–18 years old (n = 534) from selected public and Adventist middle-high schools reported their dietary intake in a web-based food frequency questionnaire (FFQ); anthropometrics were measured during school visits. Diet quality (DQI-I) and avocado intake were calculated from the FFQ; BMI z-scores (BMIz), waist-to-height ratio (WHtR), and fat mass (FM), fat-free mass (FFM), and %body fat (%BF) were determined from the anthropometric data. Compared to non-consumers, avocado consumers had significantly higher covariate-adjusted mean scores on total DQI-I (68.3 vs. 64.6) and energy-adjusted mean scores on variety (18.8 vs. 18.0) and adequacy (36.4 vs. 33.4). Avocado consumption was significantly associated with DQI-I components adequacy (β [SE] = 0.11 [0.03]) and moderation (β [SE] = 0.06 [0.03]) but not with BMIz, WHtR, FM, FFM, and %BF. Mean intakes of fruits, vegetables, and plant protein foods, total and vegetable proteins, dietary fiber, retinol, vitamin C, calcium, magnesium, iron, and potassium were significantly higher for avocado consumers; saturated and trans fats intakes were significantly higher for non-consumers. In our adolescent population, avocado consumption was associated with higher diet quality and intake of plant-based foods and shortfall nutrients, but not with measures of obesity and body composition. Studies are needed to determine the optimal amount of avocado that would confer health benefits during adolescence.
“Five Keys to Safer Food” and COVID-19
Authors: San Onofre Nadia , Soler Carla , Merino-Torres J. Francisco , Soriano Jose M.
Published: December 2021

On 11 March 2020, coronavirus disease 2019 (COVID-19) was declared a pandemic by the World Health Organization (WHO) and, up to 18:37 a.m. on 9 December 2021, it has produced 268,440,530 cases and 5,299,511 deaths. This disease, in some patients, included pneumonia and shortness of breath, being transmitted through droplets and aerosols. To date, there is no scientific literature to justify transmission directly from foods. In this review, we applied the precautionary principle for the home and the food industry using the known “Five Keys to Safer Food” manual developed by the World Health Organization (WHO) and extended punctually in its core information from five keys, in the light of new COVID-19 evidence, to guarantee a possible food safety tool.
Effects of Different Types of Carbohydrates on Arterial Stiffness: A Comparison of Isomaltulose and Sucrose
Authors: Kobayashi Ryota , Sakazaki Miki , Nagai Yukie , Asaki Kenji , Hashiguchi Takeo , Negoro Hideyuki
Published: December 2021

Increased arterial stiffness during acute hyperglycemia is a risk factor for cardiovascular disease, but the type of carbohydrate that inhibits it is unknown. The purpose of this study was to determine the efficacy of low-glycemic-index isomaltulose on arterial stiffness during hyperglycemia in middle-aged and older adults. Ten healthy middle-aged and older adult subjects orally ingested a solution containing 25 g of isomaltulose (ISI trial) and sucrose (SSI trial) in a crossover study. In the SSI trial, the brachial–ankle (ba) pulse wave velocity (PWV) increased 30, 60, and 90 min after ingestion compared with that before ingestion (p < 0.01); however, in the ISI trial, the baPWV did not change after ingestion compared with that before ingestion. Blood glucose levels 30 min after intake were lower in the ISI trial than in the SSI trial (p < 0.01). The baPWV and systolic blood pressure were positively correlated 90 min after isomaltulose and sucrose ingestion (r = 0.640, p < 0.05). These results indicate that isomaltulose intake inhibits an acute increase in arterial stiffness. The results of the present study may have significant clinical implications on the implementation of dietary programs for middle-aged and elderly patients.
Trends in Taxonomy of Chagas Disease Vectors (Hemiptera, Reduviidae, Triatominae): From Linnaean to Integrative Taxonomy
Authors: Alevi Kaio Cesar Chaboli , de Oliveira Jader , da Silva Rocha Dayse , Galvão Cleber
Published: December 2021

Chagas disease is a neglected tropical disease caused by the protozoan Trypanosoma cruzi and transmitted mainly by members of the subfamily Triatominae. There are currently 157 species, grouped into 18 genera and five tribes. Most descriptions of triatomine species are based on classical taxonomy. Facing evolutionary (cryptic speciation and phenotypic plasticity) and taxonomic (more than 190 synonymizations) problems, it is evident that integrative taxonomy studies are an important and necessary trend for this group of vectors. Almost two-and-a-half centuries after the description of the first species, we present for the first time the state-of-the-art taxonomy of the whole subfamily, covering from the initial classic studies to the use of integrative taxonomy.
Secreted MbovP0145 Promotes IL-8 Expression through Its Interactive β-Actin and MAPK Activation and Contributes to Neutrophil Migration
Authors: Lu Doukun , Zhang Hui , Zhang Yiqiu , Zhao Gang , Anwar Khan Farhan , Chen Yingyu , Hu Changmin , Yang Liguo , Chen Huanchun , Guo Aizhen
Published: December 2021

Mycoplasma bovis (M. bovis) is an important pathogen of cattle responsible for huge economic losses in the dairy and beef industries worldwide. The proteins secreted by M. bovis are mainly related to its adhesion, invasion, virulence, and intracellular survival and play a role in mycoplasma–host interactions. In our previous study, we found MbovP0145, a secreted protein present in the M. bovis secretome, but little is known about its function. In this study, we assessed the inflammatory characteristics and underlined mechanism of this inflammation of recombinant MbovP0145 (rMbovP0145). For this, bovine lung epithelial cells (EBL) were stimulated by rMbovP0145 to see the IL-8 production in a time- and dose-dependent manner. We observed that rMbovP0145 increased the production of IL-8 via ERK1/2 and P38 pathway activation. Further, the effect of the M. bovis ΔMbov_0145 mutant and its complementary strain on IL-8 mRNA expression was also confirmed. A pulldown assay of the GST-tagged MbovP0145 protein with mass spectrometry demonstrated that β-actin could specifically interact with rMbovP0145 to mediate the IL-8 signaling. As knockdown of β-actin expression with RNA interference in EBL cells decreased the mRNA expression of IL-8 and the phosphorylated ERK1/2 and P38 proteins, whereas disrupted actin polymerization by cytochalasin D led to a significantly higher IL-8 expression and MAPK phosphorylation in rMbovP0145-stimulated cells. Compared to M. bovis HB0801 and its complementary strain, the culture supernatant of EBL cells infected with the M. bovis ΔMbov_0145 mutant induced less neutrophil migration to the lower chamber in a transwell system. In conclusion, MbovP0145 promoted IL-8 expression by interacting with β-actin through activation of the MAPK pathway, thus contributing to neutrophil migration.
Comparative Diagnostic Performance of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Kit for the Rapid Detection of SARS-CoV-2
Authors: Domnich Alexander , Orsi Andrea , Panatto Donatella , De Pace Vanessa , Ricucci Valentina , Caligiuri Patrizia , Guarona Giulia , Chessa Valerio , Ferone Diego , Boccotti Simona , Bruzzone Bianca , Icardi Giancarlo
Published: December 2021

Although the reverse transcription-polymerase chain reaction (RT-PCR) is considered a standard-of-care assay for the laboratory diagnosis of SARS-CoV-2, several limitations of this method have been described. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is an alternative molecular assay and is potentially able to overcome some intrinsic shortcomings of RT-PCR. In this study, we evaluated the diagnostic performance of the novel HG COVID-19 RT-LAMP assay. In this retrospective analysis, a total of 400 routinely collected leftover nasopharyngeal samples with a known RT-PCR result were tested by means of the HG COVID-19 RT-LAMP assay. The overall sensitivity and specificity values of HG COVID-19 RT-LAMP versus RT-PCR were 97.0% (95% CI: 93.6–98.9%) and 98.5% (95% CI: 95.7–99.7%), respectively. Inter-assay agreement was almost perfect (κ = 0.96). Concordance was perfect in samples with high viral loads (cycle threshold < 30). The average time to a positive result on RT-LAMP was 17 min. HG COVID-19 RT-LAMP is a reliable molecular diagnostic kit for detecting SARS-CoV-2, and its performance is comparable to that of RT-PCR. Shorter turnaround times and the possibility of performing molecular diagnostics in the point-of-care setting make it a valuable option for facilities without sophisticated laboratory equipment.
6′-O-Lactose Ester Surfactants as an Innovative Opportunity in the Pharmaceutical Field: From Synthetic Methods to Biological Applications
Authors: Verboni Michele , Lucarini Simone , Duranti Andrea
Published: December 2021

Glycolipid surfactants are biocompatible and biodegradable compounds characterized by potential applications in various sectors including pharmaceuticals, cosmetics, agriculture, and food production. A specific overview regarding synthetic methodologies and properties of 6′-lactose-based surfactants is presented herein, particularly all the synthetic approaches to this class of lactose esters, such as enzymatic and traditional organic syntheses. Moreover, detailed descriptions of physicochemical data and biocompatibility properties of these molecules, that is, surface tension, critical micelle concentration, emulsifying ability, foaming, particle size distribution, biocompatibility, and safety, are described. Biological applications with a focus on permeability enhancing, antimicrobial activity, and antibiofilm properties of 6′-lactose-based esters are also reported.
Design and Synthesis of Hybrid Compounds as Epigenetic Modifiers
Authors: Lopes Juliana Romano , Prokopczyk Igor Muccilo , Gerlack Max , Man Chin Chung , Santos Jean Leandro Dos
Published: December 2021

Epigenetic modifiers acting through polypharmacology mechanisms are promising compounds with which to treat several infectious diseases. Histone deacetylase (HDAC) enzymes, mainly class I, and extra-terminal bromodomains (BET) are involved in viral replication and the host response. In the present study, 10 compounds were designed, assisted by molecular docking, to act against HDAC class I and bromodomain-4 (BRD4). All the compounds were synthesized and characterized by analytical methods. Enzymatic assays were performed using HDAC-1, -4, and -11 and BRD4. Compounds (2–10) inhibited both HDAC class I, mainly HDAC-1 and -2, and reduced BRD4 activity. For HDAC-1, the inhibitory effect ranged from 8 to 95%, and for HDAC-2, these values ranged from 10 to 91%. Compounds (2–10) decreased the BRD4 activity by up to 25%. The multi-target effects of these compounds show desirable properties that could help to combat viral infections by acting through epigenetic mechanisms.
Therapeutic Response of CCKBR-Positive Tumors to Combinatory Treatment with Everolimus and the Radiolabeled Minigastrin Analogue [177Lu]Lu-PP-F11N
Authors: Grzmil Michal , Imobersteg Stefan , Blanc Alain , Frank Stephan , Schibli Roger , Béhé Martin P.
Published: December 2021

The inhibition of the mammalian target of rapamycin complex 1 (mTORC1) by everolimus (RAD001) was recently shown to enhance the tumor uptake of radiolabeled minigastrin. In this paper, we investigate if this finding can improve the in vivo therapeutic response to [177Lu]Lu-PP-F11N treatment. The N-terminal DOTA-conjugated gastrin analogue PP-F11N (DOTA-(DGlu)6-Ala-Tyr-Gly-Trp-Nle-Asp-Phe) was used to evaluate treatment efficacy in the human A431/CCKBR xenograft nude mouse model in combination with RAD001. Both RAD001 and [177Lu]Lu-PP-F11N single treatments as well as their combination inhibited tumor growth and increased survival. In concomitantly treated mice, the average tumor size and median survival time were significantly reduced and extended, respectively, as compared to the monotherapies. The histological analysis of kidney and stomach dissected after treatment with RAD001 and [177Lu]Lu-PP-F11N did not indicate significant adverse effects. In conclusion, our study data demonstrate the potential of mTORC1 inhibition to substantially improve the therapeutic efficacy of radiolabeled minigastrin analogues in CCKBR-positive cancers.
Intra-Articular Drug Delivery for Osteoarthritis Treatment
Authors: Cao Yifeng , Ma Yifeng , Tao Yi , Lin Weifeng , Wang Ping
Published: December 2021

Osteoarthritis (OA) is the most prevalent degenerative joint disease affecting millions of people worldwide. Currently, clinical nonsurgical treatments of OA are only limited to pain relief, anti-inflammation, and viscosupplementation. Developing disease-modifying OA drugs (DMOADs) is highly demanded for the efficient treatment of OA. As OA is a local disease, intra-articular (IA) injection directly delivers drugs to synovial joints, resulting in high-concentration drugs in the joint and reduced side effects, accompanied with traditional oral or topical administrations. However, the injected drugs are rapidly cleaved. By properly designing the drug delivery systems, prolonged retention time and targeting could be obtained. In this review, we summarize the drugs investigated for OA treatment and recent advances in the IA drug delivery systems, including micro- and nano-particles, liposomes, and hydrogels, hoping to provide some information for designing the IA injected formulations.
Multi-Lane Mirror for Broadband Applications of the Betatron X-ray Source
Authors: Raclavský Marek , Khakurel Krishna P. , Chaulagain Uddhab , Lamač Marcel , Nejdl Jaroslav
Published: December 2021

A new generation of small-scale ultrafast X-ray sources is rapidly emerging. Laser-driven betatron radiation represents an important class of such ultrafast X-ray sources. With the sources driving towards maturity, many important applications in material and biological sciences are expected to be carried out. While the last decade mainly focused on the optimization of the source properties, the development of such sources into user-oriented beamlines in order to explore the potential applications has recently taken off and is expected to grow rapidly. An important aspect in the realization of such beamlines will be the implementation of proper X-ray optics. Here, we present the design of a multi-lane X-ray mirror as a versatile focusing device covering a wide spectral range of betatron X-rays. The expected photon flux in the focal plane of such optics was also estimated through geometrical simulations.
New Insights into Interspecific Hybridization in Lemna L. Sect. Lemna (Lemnaceae Martinov)
Authors: Braglia Luca , Breviario Diego , Gianì Silvia , Gavazzi Floriana , De Gregori Jacopo , Morello Laura
Published: December 2021

Duckweeds have been increasingly studied in recent years, both as model plants and in view of their potential applications as a new crop in a circular bioeconomy perspective. In order to select species and clones with the desired attributes, the correct identification of the species is fundamental. Molecular methods have recently provided a more solid base for taxonomy and yielded a consensus phylogenetic tree, although some points remain to be elucidated. The duckweed genus Lemna L. comprises twelve species, grouped in four sections, which include very similar sister species. The least taxonomically resolved is sect. Lemna, presenting difficulties in species delimitation using morphological and even barcoding molecular markers. Ambiguous species boundaries between Lemna minor L. and Lemna japonica Landolt have been clarified by Tubulin Based Polymorphism (TBP), with the discovery of interspecific hybrids. In the present work, we extended TBP profiling to a larger number of clones in sect. Lemna, previously classified using only morphological features, in order to test that classification, and to investigate the possible existence of other hybrids in this section. The analysis revealed several misidentifications of clones, in particular among the species L. minor, L. japonica and Lemna gibba L., and identified six putative ‘L. gibba’ clones as interspecific hybrids between L. minor and L. gibba.
Secretory Patterns in Colleters of Apocynaceae
Authors: Ribeiro Josiana Cristina , Tölke Elisabeth Dantas , Demarco Diego
Published: December 2021

Colleters of Apocynaceae are glands related to different types of protection of vegetative and floral meristems through the production of mucilage or a mixture of many different compounds. Although several anatomical papers have shown histological and histochemical aspects of colleters of the family, almost nothing is known about their secretory process. In this study, we analyzed two types of colleters in Apocynaceae: one produces mucilage and lipophilic compounds, while the other produces an exclusively mucilaginous secretion. The secretory epidermis of the colleters of Allamanda schottii and Blepharodon bicuspidatum has a dense cytoplasm with organelles responsible for the production of mucilage and lipids. This heterogeneous secretion is released through granulocrine and eccrine mechanisms and is temporarily stored in a subcuticular space before crossing the cuticle. Conversely, colleters of Mandevilla splendens and Peplonia axillaris produce only mucilage and have a very different secretory apparatus. The mechanism of secretion is granulocrine, and the exudate is firstly accumulated in a large periplasmic space and later in an intramural space before crossing the cuticle. Notably, the structure of the cuticle varies according to the secretion composition. Although the colleters of the family are histologically similar, this study demonstrates a metabolic and subcellular variability previously unknown for Apocynaceae.
The Amino-Proximal Region of the Coat Protein of Cucumber Vein Yellowing Virus (Family Potyviridae) Affects the Infection Process and Whitefly Transmission
Authors: Lindenau Svenja , Winter Stephan , Margaria Paolo
Published: December 2021

Most plant viruses rely on vector transmission for their spread and specific interactions between vector and virus have evolved to regulate this relationship. The whitefly Bemisia tabaci- transmitted cucumber vein yellowing virus (CVYV; genus Ipomovirus, family Potyviridae) is endemic in the Mediterranean Basin, where it causes significant losses in cucurbit crops. In this study, the role of the coat protein (CP) of CVYV for B. tabaci transmission and plant infection was investigated using a cloned and infectious CVYV cDNA and a collection of point and deletion mutants derived from this clone. Whitefly transmission of CVYV was abolished in a deletion mutant lacking amino acids in position 93–105 of the CP. This deletion mutant caused more severe disease symptoms compared to the cDNA clone representing the wild-type (wt) virus and movement efficiency was likewise affected. Two virus mutants carrying a partially restored CP were transmissible and showed symptoms comparable to the wt virus. Collectively, our data demonstrate that the N-terminus of the CVYV CP is a determinant for transmission by the whitefly vector and is involved in plant infection and symptom expression.
Short-Term Waterlogging in Citrus Rootstocks
Authors: Pérez-Jiménez Margarita , Pérez-Tornero Olaya
Published: December 2021

Changes in climate are provoking flooding events that cause waterlogging in the fields. Citrus are mainly cultivated in areas with a high susceptibility to climate change. Therefore, it is vital to explore their responses to these events to anticipate future challenges by means of genetic improvement of the commercial rootstocks. In this experiment, three popular commercial rootstocks, namely ‘Cleopatra’ (C. reshni Hort. Ex Tanaka), C. macrophylla, and ‘Forner Alcaide no. 5′ (Citrus reshni Hort. Ex Tanaka × Poncirus trifoliata), were evaluated after being submitted to short-term waterlogging and a period of recovery of 7 days in each case. Photosynthesis rate and stomatal conductance decreased in ‘Cleopatra’, while in the other two genotypes they were maintained (C. macrophylla) or restored after recovery (‘Forner Alcaide no. 5′’). Relative water content and chlorophylls also decreased in ‘Cleopatra’. This indicates a deeper effect of flooding in ‘Cleopatra’, which suffered changes during flooding that were also sustained during the recovery phase. This did not occur in the other two rootstocks, since they showed signs of recovery for those parameters that decreased during waterlogging.
Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling
Authors: Zuo Guanglei , Je Kang-Hoon , Guillen Quispe Yanymee N. , Shin Kyong-Oh , Kim Hyun Yong , Kim Kang Hyuk , Arce Paul H. Gonzales , Lim Soon Sung
Published: December 2021

We previously reported that Lepechinia meyenii (Walp.) Epling has antioxidant and aldose reductase (AR) inhibitory activities. In this study, L. meyenii was extracted in a 50% MeOH and CH2Cl2/MeOH system. The active extracts of MeOH and 50% MeOH were subjected to fractionation, followed by separation using high-speed counter-current chromatography (HSCCC) and preparative HPLC. Separation and identification revealed the presence of caffeic acid, hesperidin, rosmarinic acid, diosmin, methyl rosmarinate, diosmetin, and butyl rosmarinate. Of these, rosmarinic acid, methyl rosmarinate, and butyl rosmarinate possessed remarkable antioxidant and AR inhibitory activities. The other compounds were less active. In particular, rosmarinic acid is the key contributor to the antioxidant and AR inhibitory activities of L. meyenii; it is rich in the MeOH extract (333.84 mg/g) and 50% MeOH extract (135.41 mg/g) of L. meyenii and is especially abundant in the EtOAc and n-BuOH fractions (373.71–804.07 mg/g) of the MeOH and 50% MeOH extracts. The results clarified the basis of antioxidant and AR inhibitory activity of L. meyenii, adding scientific evidence supporting its traditional use as an anti-diabetic herbal medicine. The HSCCC separation method established in this study can be used for the preparative separation of rosmarinic acid from natural products.
Chiral Nematic Cellulose Nanocrystal Films Cooperated with Amino Acids for Tunable Optical Properties
Authors: Xiao Xiao , Chen Jie , Ling Zhe , Guo Jiaqi , Huang Jianbin , Ma Jianfeng , Jin Zhi
Published: December 2021

The exploration of functional materials relies greatly on the understanding of material structures and nanotechnologies. In the present work, chiral nematic cellulose nanocrystal (CNC) films were prepared by incorporation with four types of amino acids (AAs, glycine, histidine, phenylalanine, and serine) via evaporation-induced self-assembly. The films present ideal iridescence and birefringence that can be tuned by the amount of AAs added. The intercalation of AAs enlarged the pitch values, contributing to the red-shift trend of the reflective wavelength. Among the AAs, serine presented the most compatible intercalation into cellulose crystals. Interestingly, histidine and phenylalanine composite films showed high shielding capabilities of UV light in diverse wavelength regions, exhibiting multi-optical functions. The sustainable preparation of chiral nematic CNC films may provide new strategies for materials production from biocompatible lignocellulose.
Chitosan Reinforced with Kenaf Nanocrystalline Cellulose as an Effective Carrier for the Delivery of Platelet Lysate in the Acceleration of Wound Healing
Authors: Bhatnagar Payal , Law Jia Xian , Ng Shiow-Fern
Published: December 2021

The clinical use of platelet lysate (PL) in the treatment of wounds is limited by its rapid degradation by proteases at the tissue site. This research aims to develop a chitosan (CS) and kenaf nanocrystalline cellulose (NCC) hydrogel composite, which intend to stabilize PL and control its release onto the wound site for prolonged action. NCC was synthesized from raw kenaf bast fibers and incorporated into the CS hydrogel. The physicochemical properties, in vitro cytocompatibility, cell proliferation, wound scratch assay, PL release, and CS stabilizing effect of the hydrogel composites were analyzed. The study of swelling ratio (>1000%) and moisture loss (60–90%) showed the excellent water retention capacity of the CS-NCC-PL hydrogels as compared with the commercial product. In vitro release PL study (flux = 0.165 mg/cm2/h) indicated that NCC act as a nanofiller and provided the sustained release of PL compared with the CS hydrogel alone. The CS also showed the protective effect of growth factor (GF) present in PL, thereby promoting fast wound healing via the formulation. The CS-NCC hydrogels also augmented fibroblast proliferation in vitro and enhanced wound closures over 72 h. This study provides a new insight on CS with renewable source kenaf NCC as a nanofiller as a potential autologous PL wound therapy.
Effect of Low Molecular Weight Oxidized Materials and Nitrogen Groups on Adhesive Joints of Polypropylene Treated by a Cold Atmospheric Plasma Jet
Authors: Polášková Kateřina , Klíma Miloš , Jeníková Zdeňka , Blahová Lucie , Zajíčková Lenka
Published: December 2021

Polypropylene is a typical representative of synthetic polymers that, for many applications including adhesive joints, requires an increase in wettability and chemical surface reactivity. Plasma processing offers efficient methods for such surface modifications. A particular disadvantage of the plasma jets can be the small plasma area. Here, we present a cold atmospheric plasma radio-frequency slit jet developed with a width of 150 mm applied to polypropylene plasma treatment in Ar, Ar/O2 and Ar/N2 We identified two main parameters influencing the tensile strength of adhesive joints mediated by epoxy adhesive DP 190, nitrogen content, and the amount of low molecular weight oxidized materials (LMWOMs). Nitrogen functional groups promoted adhesion between epoxy adhesive DP 190 and the PP by taking part in the curing process. LMWOMs formed a weak boundary layer, inhibiting adhesion by inducing a cohesive failure of the joint. A trade off between these two parameters determined the optimized conditions at which the strength of the adhesive joint increased 4.5 times. Higher adhesion strength was previously observed when using a translational plasma gliding arc plasma jet with higher plasma gas temperatures, resulting in better cross linking of polymer chains caused by local PP melting.
Esterification of Cellulose with Long Fatty Acid Chain through Mechanochemical Method
Authors: Lease Jacqueline , Kawano Tessei , Andou Yoshito
Published: December 2021

Mechanochemical reaction, a green synthetic esterification route was utilized to prepare long-chain cellulose esters from microcrystalline cellulose. The influence of reaction conditions such as reaction temperature and time were elucidated. Only low dosage of oleic acid, 1-butyl-3-metylimidazolium acetate, and p-toluenesulfonyl chloride were required. The success of modification reaction was confirmed by Fourier transforms infrared spectroscopy as a new absorbance peak at 1731 cm−1 was observed, which indicated the formation of carbonyl group (C=O). Solid-state nuclear magnetic resonance was also performed to determine the structural property and degree of substitution (DS) of the cellulose oleate. Based on the results, increasing reaction temperature and reaction time promoted the esterification reaction and DS. DS values of cellulose oleates slightly decreased after 12 h reaction time. Besides, X-ray diffraction analysis showed the broadening of the diffraction peaks and thermal stability decreased after esterification. Hence, the findings suggested that grafting of oleic acid’s aliphatic chain onto the cellulose backbone lowered the crystallinity and thermal stability.
Rapid Ultrasound-Assisted Starch Extraction from Sago Pith Waste (SPW) for the Fabrication of Sustainable Bioplastic Film
Authors: Tan Shiou Xuan , Andriyana Andri , Lim Steven , Ong Hwai Chyuan , Pang Yean Ling , Ngoh Gek Cheng
Published: December 2021

The present study was conducted to optimize the extraction yield of starch from sago (Metroxylon sagu) pith waste (SPW) with the assistance of ultrasound ensued by the transformation of extracted starch into a higher value-added bioplastic film. Sago starch with extraction yield of 71.4% was successfully obtained using the ultrasound-assisted extraction, with the following conditions: particle size <250 µm, solid loading of 10 wt.%, ultrasonic amplitude of 70% and duty cycle of 83% in 5 min. The rapid ultrasound approach was proven to be more effective than the conventional extraction with 60.9% extraction yield in 30 min. Ultrasound-extracted starch was found to exhibit higher starch purity than the control starch as indicated by the presence of lower protein and ash contents. The starch granules were found to have irregular and disrupted surfaces after ultrasonication. The disrupted starch granules reduced the particle size and increased the swelling power of starch which was beneficial in producing a film-forming solution. The ultrasound-extracted sago starch was subsequently used to prepare a bioplastic film via solution casting method. A brownish bioplastic film with tensile strength of 0.9 ± 0.1 MPa, Young’s modulus of 22 ± 0.8 MPa, elongation at break of 13.6 ± 2.0% and water vapour permeability (WVP) of 1.11 ± 0.1 × 10−8 g m−1 s−1 Pa−1 was obtained, suggesting its feasibility as bioplastic material. These findings provide a means of utilization for SPW which is in line with the contemporary trend towards greener and sustainable products and processes.
Effects of the Washing Time and Washing Solution on the Biocompatibility and Mechanical Properties of 3D Printed Dental Resin Materials
Authors: Hwangbo Na-Kyung , Nam Na-Eun , Choi Jong-Hoon , Kim Jong-Eun
Published: December 2021

Three-dimensional (3D) printing technology is highly regarded in the field of dentistry. Three-dimensional printed resin restorations must undergo a washing process to remove residual resin on the surface after they have been manufactured. However, the effect of the use of different washing solutions and washing times on the biocompatibility of the resulting resin restorations is unclear. Therefore, we prepared 3D-printed denture teeth and crown and bridge resin, and then washed them with two washing solutions (isopropyl alcohol and tripropylene glycol monomethyl ether) using different time points (3, 5, 10, 15, 30, 60, and 90 min). After this, the cell viability, cytotoxicity, and status of human gingival fibroblasts were evaluated using confocal laser scanning. We also analyzed the flexural strength, flexural modulus, and surface SEM imaging. Increasing the washing time increased the cell viability and decreased the cytotoxicity (p < 0.001). Confocal laser scanning showed distinct differences in the morphology and number of fibroblasts. Increasing the washing time did not significantly affect the flexural strength and surface, but the flexural modulus of the 90 min washing group was 1.01 ± 0.21 GPa (mean ± standard deviation), which was lower than that of all the other groups and decreased as the washing time increased. This study confirmed that the washing time affected the biocompatibility and mechanical properties of 3D printed dental resins.
Optical and Thermal Remote Sensing for Monitoring Agricultural Drought
Authors: Qin Qiming , Wu Zihua , Zhang Tianyuan , Sagan Vasit , Zhang Zhaoxu , Zhang Yao , Zhang Chengye , Ren Huazhong , Sun Yuanheng , Xu Wei , Zhao Cong
Published: December 2021

By effectively observing the land surface and obtaining farmland conditions, satellite remote sensing has played an essential role in agricultural drought monitoring over past decades. Among all remote sensing techniques, optical and thermal remote sensing have the most extended history of being utilized in drought monitoring. The primary goal of this paper is to illustrate how optical and thermal remote sensing have been and will be applied in the monitoring, assessment, and prediction of agricultural drought. We group the methods into four categories: optical, thermal, optical and thermal, and multi-source. For each category, a concise explanation is given to show the inherent mechanisms. We pay special attention to solar-induced chlorophyll fluorescence, which has great potential in early drought detection. Finally, we look at the future directions of agricultural drought monitoring, including (1) early detection; (2) spatio-temporal resolution; (3) organic combination of multi-source data; and (4) smart prediction and assessment based on deep learning and cloud computing.
S2Looking: A Satellite Side-Looking Dataset for Building Change Detection
Authors: Shen Li , Lu Yao , Chen Hao , Wei Hao , Xie Donghai , Yue Jiabao , Chen Rui , Lv Shouye , Jiang Bitao
Published: December 2021

Building-change detection underpins many important applications, especially in the military and crisis-management domains. Recent methods used for change detection have shifted towards deep learning, which depends on the quality of its training data. The assembly of large-scale annotated satellite imagery datasets is therefore essential for global building-change surveillance. Existing datasets almost exclusively offer near-nadir viewing angles. This limits the range of changes that can be detected. By offering larger observation ranges, the scroll imaging mode of optical satellites presents an opportunity to overcome this restriction. This paper therefore introduces S2Looking, a building-change-detection dataset that contains large-scale side-looking satellite images captured at various off-nadir angles. The dataset consists of 5000 bitemporal image pairs of rural areas and more than 65,920 annotated instances of changes throughout the world. The dataset can be used to train deep-learning-based change-detection algorithms. It expands upon existing datasets by providing (1) larger viewing angles; (2) large illumination variances; and (3) the added complexity of rural images. To facilitate the use of the dataset, a benchmark task has been established, and preliminary tests suggest that deep-learning algorithms find the dataset significantly more challenging than the closest-competing near-nadir dataset, LEVIR-CD+. S2Looking may therefore promote important advances in existing building-change-detection algorithms.
Evaluations of Remote Sensing-Based Global Evapotranspiration Datasets at Catchment Scale in Mountain Regions
Authors: Jiang Yongshan , Liu Zhaofei
Published: December 2021

Evapotranspiration (ET) is essential for connecting ecosystems and directly affects the water consumption of forests, grasslands, and farmlands. Eight global remote sensing-based ET (RS_ET) datasets generated using satellite imagery and ground-based observations were comprehensively assessed using monthly ET time series simulated by the water balance (WB) method at the catchment scale in the Hengduan Mountain (HDM) region, including the Nu River, Lancang River, and Jinsha River basins. The complementary relationship (CR) model, which derives ET from meteorological data, was also evaluated against WB-based ET (WB_ET). In addition, WB_ET, RS_ET, and CR-based ET (CR_ET) data were used to investigate ET spatial and temporal variations at the catchment, grid, and site scale, respectively. Most RS_ET datasets accurately simulated monthly ET with an average index of agreement ranging from 0.71–0.91. The Operational Simplified Surface Energy Balance dataset outperformed other RS_ET datasets, with Nash–Sutcliffe efficiency coefficient (NSE) and Kling–Gupta efficiency values of 0.80 and 0.90, respectively. RS_ET datasets generally performed better in northern semiarid areas than in humid southern areas. The monthly ET simulation by the CR model was consistent with that of the WB_ET in the HDM region, with mean values of correlation coefficient (cc) and NSE at each site of 0.89 and 0.68, respectively. The model showed better performance in simulating monthly ET in the Lancang River Basin than in the Nu River and Lancang River basins, with mean cc and NSE of 0.92 and 0.83, respectively. Generally, annual ET trends were consistent at the catchment, grid, and site scale, as estimated by the WB method, RS_ET datasets, and CR model. It showed a significant decreasing trend in the northern semiarid region of the HDM while exhibiting an increasing trend in the humid southern region.