bluuebunny/crossref_metadata_2025 huggingface dataset:
How COVID-19 Have Changed Crowdfunding: Evidence From GoFundMe
Authors: Wang Junda , Zhang Xupin , Luo Jiebo
Published: July 2021

While the long-term effects of COVID-19 are yet to be determined, its immediate impact on crowdfunding is nonetheless significant. This study takes a computational approach to more deeply comprehend this change. Using a unique data set of all the campaigns published over the past two years on GoFundMe, we explore the factors that have led to the successful funding of a crowdfunding project. In particular, we study a corpus of crowdfunded projects, analyzing cover images and other variables commonly present on crowdfunding sites. Furthermore, we construct a classifier and a regression model to assess the significance of features based on XGBoost. In addition, we employ counterfactual analysis to investigate the causality between features and the success of crowdfunding. More importantly, sentiment analysis and the paired sample t-test are performed to examine the differences in crowdfunding campaigns before and after the COVID-19 outbreak that started in March 2020. First, we note that there is significant racial disparity in crowdfunding success. Second, we find that sad emotion expressed through the campaign's description became significant after the COVID-19 outbreak. Considering all these factors, our findings shed light on the impact of COVID-19 on crowdfunding campaigns.
Paradigm Shift: an Overview on Nutrigenetics and Nutrigenomics
Authors: Ciaurelli Stefano , Origlia Caterina , Adamo Ariela , Giannattasio Silvia , González Michael , Quinto Liborio , Biava Pier Mario
Published: July 2021

Nutrigenetics concerns individual differences in the reaction to food based on the genetic factors. Nutrigenomics analyses direct influences of nutrients on gene expression. Both terms are explained in our review article. Nutrigenetics and nutrigenomics require a deep understanding of nutrition, genetics and biochemistry and of new ‘omic’ technologies. It is often difficult to appreciate their relevance in the practice of preventive approaches for optimizing health, delaying onset of disease and diminishing its severity. This paper represents a review about the current research on Nutrigenomics and Nutrigenetics, and provide scientific data for a new integrated clinical approach.
Jasmonic Acid-dependent MYC Transcription Factors Bind to a Tandem G-box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses
Authors: Pérez-Alonso Marta-Marina , Sánchez-Parra Beatriz , Ortiz-García Paloma , Santamaría Estrella , Díaz Isabel , Pollmann Stephan
Published: July 2021

The indole-3-pyruvic acid pathway is the major route for auxin biosynthesis in higher plants. Tryptophan aminotransferases (TAA1/TAR) and members of the YUCCA family of flavin-containing monooxygenases catalyze the conversion of L-tryptophan via indole-3-pyruvic acid into indole-3-acetic acid (IAA). It has been described that locally produced jasmonic acid (JA) in response to mechanical wounding, triggers de novo-formation of IAA through the induction of two YUCCA genes, YUC8 and YUC9. Here, we report the direct involvement of a small number of basic helix-loop-helix transcription factors of the MYC family in this process. We show that the JA-mediated regulation of YUC8 and YUC9 gene expression depends on the abundance of MYC2, MYC3, and MYC4. In support of this observation, seedlings of myc knockout mutants displayed a strongly reduced response to JA-mediated IAA formation. In addition, transactivation assays provided experimental evidence for the binding of the MYC transcription factors to a particular tandem G-box motif abundant in the promoter regions of YUC8 and YUC9, but not in those of the other YUCCA genes. Moreover, we clearly demonstrate that YUC8ox and YUC9ox overexpressing plants show less damage after spider mite infestation, thereby underlining a role of auxin in plant responses toward biotic stress cues.
In Vitro and In Vivo Evaluation of Human Adenovirus Type 49 as a Vector for Therapeutic Applications
Authors: Bates Emily A. , Counsell John R. , Alizert Sophie , Baker Alexander T. , Suff Natalie , Bradshaw Angela C. , Waddington Simon N. , Nicklin Stuart A. , Baker Andrew H. , Parker Alan L.
Published: July 2021

The human adenovirus phylogenetic tree is split across seven species (A-G). Species D adenoviruses offer potential advantages for gene therapy applications, with low rates of preexisting immunity detected across screened populations. However, many aspects of the basic virology of species D, such as their cellular tropism, receptor usage and in vivo biodistribution profile, remain unknown. Here, we have characterized human adenovirus type 49 (HAdV-D49), a relatively understudied species D member. We report that HAdV-D49 does not appear to use a single pathway to gain cell entry but appears able to interact with various surface molecules for entry. As such, HAdV-D49 can transduce a broad range of cell types in vitro, with variable engagement of blood coagulation FX. Interestingly, when comparing in vivo biodistribution to adenovirus type 5, HAdV-D49 vectors show reduced liver targeting whilst maintaining transduction of lung and spleen. Overall, this presents HAdV-D49 as a robust viral vector platform for ex vivo manipulation of human cells and for in vivo applications where the therapeutic goal is to target the lung or gain access to immune cells in the spleen whilst avoiding liver interactions, such as intravascular vaccine applications.
ACTonFood. Acceptance and Commitment Therapy-Based Group Treatment Compared to Cognitive Behavioral Therapy-Based Group Treatment for Weight Maintenance: An Individually Randomized Group Treatment Trial
Authors: Cattivelli Roberto , Guerrini Usubini Anna , Manzoni Gian Mauro , Vailati Riboni Francesco , Pietrabissa Giada , Musetti Alessandro , Franceschini Christian , Varallo Giorgia , Spatola Chiara A.M. , Giusti Emanuele , Castelnuovo Gianluca , Molinari Enrico
Published: July 2021

The purpose of this Individually Randomized Group Treatment Trial was to compare an Acceptance and Commitment Therapy-based (ACT) group intervention and a Cog-nitive Behavioral Therapy-based (CBT) group intervention for weight loss maintenance in a sample of adult patients with obesity seeking treatment for weight loss. 155 over-weight adults (BMI: Kg/m2= 43.8[6.8]) attending a multidisciplinary rehabilitation program for weight loss were randomized into two conditions: ACT and CBT. Demo-graphical, physical, and clinical data were assessed at the beginning of the program (t0), at discharge (t1), and at 6-month follow-up (t2). The following measures were ad-ministered: The Acceptance and Action Questionnaire-II (AAQ-II) and the Clinical Outcome in Routine Evaluation-Outcome Measure (CORE-OM). Generalized linear mixed models were performed to assess differences between groups. Moderation ef-fects for gender and eating disorders (ED) have been considered. From baseline to dis-charge no significant differences between interventions were found, with the only ex-ception of an improvement in the CORE-OM total score and in the CORE-OM subjective well-being subscale for those in the CBT condition. From discharge to follow-up ACT group participants showed significant results in terms of weight loss maintenance, CORE-OM total score, and CORE-OM and AAQ-II’s wellbeing, symptoms, and psy-chological problems subscales. Gender moderated the effects of time and intervention on the CORE-OM’ subscale reporting the risk for self-harm or harm others. The pres-ence of an eating disorder moderated the effect of time and intervention on the CORE-OM total score, on the CORE-Om’ symptoms and psychological problems sub-scales, and on the AAQ-II. Patients who received the ACT intervention were more likely to achieve a ≥5% weight loss from baseline to follow-up and to maintain the weight loss after discharge. The ACT intervention was thus effective in maintaining weight loss over time.
An Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication Through Incorporating Mx1 Protein
Authors: Guo Changjun , He Jian , Li Zhi-Min , Wang Yuanyuan , nan Chen nan , Weng Shao-Ping , He Jian-Guo
Published: July 2021

Exosomes are associated with cancer progression, pregnancy, cardiovascular diseases, central nervous system–related diseases, immune responses and viral pathogenicity. However, study on the role of exosomes in the immune response of teleost fish, especially antiviral immunity, is limited. Herein, serum-derived exosomes from mandarin fish were used to investigate antiviral effect for the exosomes of teleost fish. Exosomes were isolated from mandarin fish serum by ultracentrifugation could internalize into Mandarin fish fry (MFF-1) cells and inhibited Infectious spleen and kidney necrosis virus (ISKNV) infection. To further investigated the underlying mechanisms of exosomes in inhibiting ISKNV infection. The protein composition of serum-derived exosomes was by analysis mass spectrometry and found that myxovirus resistance 1 (Mx1) was incorporated in the exosomes. Furthermore, the scMx1 protein was proved transferred to the recipient cells though the exosomes. Our results found that the serum-derived exosomes from mandarin fish could inhibit ISKNV replication and suggested an underlying mechanism of the serum-derived exosomes antivirus is that serum-derived exosomes incorporation of the Mx1 protein into exosomes and delivery into recipient cells. This study provided an evidence for the important antiviral role of exosomes in the immune system of teleost fish.
Effects of Caudal Autotomy on the Locomotor Performance of Micrablepharus Atticolus (Squamata, Gymnophthalmidae)
Authors: Silva Naiane Arantes , Caetano Gabriel Henrique O. , Campelo Pedro Henrique , Cavalcante Vitor Hugo Gomes Lacerda , Godinho Leandro Braga , Miles Donald Bailey , Paulino Henrique Monteiro , da Silva Júlio Miguel Alvarenga , de Souza Bruno Araújo , Silva Hosmano Batista Ferreira , Colli Guarino Rinaldi
Published: July 2021

Caudal autotomy is a dramatic adaptation used by many lizard species to evade predators. Most studies to date indicate that caudal autotomy impairs lizard locomotor performance. Surprisingly, some species bearing the longest tails show negligible impacts of caudal autotomy on sprint speed. Part of this variation has been attributed to lineage effects. For the first time, we model the effects of caudal autotomy on the locomotor performance of a gymnophthalmid lizard, Micrablepharus atticolus, characterized by a long and bright blue tail. To improve model accuracy, we incorporated the effects of several covariates. We found that body temperature, pregnancy, mass, collection site, and the length of the regenerated portion of the tail were the most important predictors of locomotor performance in Micrablepharus atticolus. However, sprint speed was unaffected by tail loss. Apparently, the long tail of M. atticolus is more useful when using undulation amidst the leaf litter and not when using quadrupedal locomotion on a flat surface. Our findings highlight the intricate relationships among physiological, morphological, and behavioral traits. We suggest that future studies about the impacts of caudal autotomy among long-tailed lizards should consider the role of different microhabitats/substrates on locomotor performance, using laboratory conditions that closely mimic their natural environments.
Monitoring of serum levels of infliximab and anti-infliximab antibodies and its role in optimizing therapy of inflammatory bowel disease in children
Authors:   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Illarionov A.S. , Petrichuk S.V. ,   National Medical Research Center of Children's Health, Moscow, Russian Federation, Fisenko A.P. ,   National Medical Research Center of Children's Health, Moscow, Russian Federation, Radygina T.V. ,   National Medical Research Center of Children's Health, Moscow, Russian Federation, Potapov A.S. ,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation,   National Medical Research Center of Children's Health, Moscow, Russian Federation, Kuptsova D.G. ,   National Medical Research Center of Children's Health, Moscow, Russian Federation, Surkov A.N. ,   National Medical Research Center of Children's Health, Moscow, Russian Federation, Konopleva T.N. ,   National Medical Research Center of Children's Health, Moscow, Russian Federation
Published: July 2021

Objective. To evaluate the prognostic value of serum levels of infliximab (IFX) and anti-IFX antibodies for predicting treatment efficacy in children with inflammatory bowel disease (IBD). Patients and methods. This study included 123 children with IBD (65 patients with ulcerative colitis (UC) and 58 patients with Crohn's disease (CD)) aged between 3 and 18 years. Children were examined upon the completion of an induction course of therapy and then after 1 year of therapy and between 1 and 3 years of treatment. The induction course was administered according to the scheme of 0–2–6 weeks; maintenance therapy was administered with 6-week and 8-week intervals. The residual level of IFX and the level of anti-IFX antibodies in serum were measured using enzyme-linked immunosorbent assay. Results. We observed a significant increase in the IFX level during clinical remission of CD (remission – 5.21 [3.32; 7.43] μg/mL; exacerbation – 2.42 [0.42; 4.51] μg/mL, p = 0.001) and UC (remission – 4.57 [3.4; 6.74] μg/mL; exacerbation – 0.63 [0.4; 3.27] μg/mL, p = 0.000). ROC analysis demonstrated high accuracy of our model based on measuring residual IFX level for distinguishing between exacerbation and remission in both CD patients (AUC = 0.812) and UC patients (AUC = 0.851). The optimal minimum level of IFX for maintaining clinical remission was 3.7 μg/mL in children with CD and 3.4 μg/mL in children with UC. Anti-IFX antibodies were detected in 18% of patients; children with exacerbation were 4.7 times more likely to have detectable level of anti-IFX antibodies than those in clinical remission. Conclusion. Monitoring of serum levels of IFX and anti-IFX antibodies is a promising method for predicting treatment efficacy and its optimization in children with IBD. Key words: inflammatory bowel disease, tumor necrosis factor-α, infliximab, residual level of infliximab, anti-infliximab antibodies
Candida-associated stomatitis in children with rheumatic diseases
Authors:   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Skakodub A.A. , Geppe N.A. ,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Admakin O.I. ,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Mamedov Ad.A. ,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Chebysheva S.N. ,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Dudnik O.V. ,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation
Published: July 2021

Objective. To improve diagnostics and treatment of candidiasis in children with rheumatic diseases. Patients and methods. We performed clinical examination of the oral cavity in 316 children with rheumatic diseases and assessed the frequency and clinical manifestations of oral candidiasis in them. Oral swabs were taken from patients with any complaints or clinical symptoms of candidiasis. Conclusion. We have developed and implemented an algorithm of treatment of oral candidiasis in children with rheumatic diseases, depending on the form of candidiasis, course of the main disease, and basic therapy used. Key words: oral candidiasis, rheumatic diseases, children
Respiratory syncytial viral infection in a child with genetic pathology
Authors:   N.N.Burdenko Voronezh State Medical University, Voronezh, Russian Federation, Kokoreva S.P. , Kotlova V.B. ,   N.N.Burdenko Voronezh State Medical University, Voronezh, Russian Federation, Makarova A.V. ,   N.N.Burdenko Voronezh State Medical University, Voronezh, Russian Federation, Razuvaev O.A. ,   N.N.Burdenko Voronezh State Medical University, Voronezh, Russian Federation, Moreplavtseva Ya.D. ,   N.N.Burdenko Voronezh State Medical University, Voronezh, Russian Federation
Published: July 2021

The relevance of respiratory syncytial virus infection is due to its widespread, severity of the course in young children with a predominant lesion of the lower respiratory tract with acute respiratory failure and absence of etiotropic therapy with proven efficacy. The risk group for severe course and mortality is made up of premature babies, children with bronchopulmonary dysplasia, hemodynamically significant congenital heart disease. In addition, according to individual indications, by the decision of the council, specific prophylaxis is carried out for newborns and premature infants with severe neuromuscular pathology, CNS injury with impaired respiratory function, patients with congenital anomalies and genetic pathology of the respiratory system, as well as congenital immunodeficiency. The article presents a description and analysis of the severe course of respiratory syncytial infection with the development of obstructive bronchitis with respiratory failure of the 2nd degree in a child aged 1 year 4 months with Charge syndrome – a severe genetic pathology, including combined congenital heart disease, choanal artesia, protein-energy malnutrition, congenital immunodeficiency. The child had bronchopulmonary dysplasia, chronic paralytic laryngeal stenosis. The disease required oxygen support and the patient's stay in the intensive care unit. The described clinical example demonstrates a severe course of respiratory syncytial virus infection with the damage of the lower respiratory tract in a child over one year old from the risk group. Key words: respiratory syncytial virus infection, children, obstructive bronchitis, bronchiolitis
THE EFFECT OF COMPANY CHARACTERISTICS TOWARDS CARBON EMISSION DISCLOSURE AND ITS IMPACT ON ECONOMIC CONSEQUENCES IN NON-FINANCIAL REGISTERED COMPANIES IN INDONESIA, MALAYSIA, THAILAND AND THE PHILIPPINES PERIOD FOR 2008-2017
Authors: Gatot Nazir Ahmad  , Roh Ajiasri  , Ari Warokka
Published: July 2021

This research was conducted to know the company's characteristics, the determining factors for the disclosure of carbon emissions, and the impact of the disclosure of carbon emissions on economic consequences. The financial result in this study is the decision-making behavior of businesses, governments, and creditors as a result of accounting reporting, in this case, environmental disclosures contained in annual reports and sustainable reports. This study's sample amounted to 45 companies registered in Indonesia, Malaysia, Thailand, and the Philippines, with an observation period of 2008 to 2017. To measure the disclosure of carbon emissions by creating a checklist based on information from the CDP. Company characteristics are proxied by profitability, leverage, size, and sales growth, while economic consequences are proxied by the bid-ask spread, trading volume, and stock price volatility. The analytical method used in this study is the Partial Least Square (PLS) method using the WARP PLS version 5.0 application. From the test results, it was found that Profitability and Size had a positive effect on disclosure of carbon emissions, growth sales had a negative effect, and leverage had no effect. Meanwhile, the impact of disclosing carbon emissions on the bid-ask spread, trading volume, and stock price volatility has a positive effect.
THE EFFECT OF TRAINING AND COMPENSATION ON PERFORMANCE EMPLOYEES WITH MOTIVATION AS A MEDIATION VARIABLE
Authors: Abdul Hakim  , I Ketut Sudiardhita  , Henry Eryanto
Published: July 2021

Training and compensation programs are provided by the company to motivate employees and improve employee performance. This study aims to analyze the effect of training and compensation on employee performance using motivation as mediation at PT. Servistama Pacific Sedana. This research uses a quantitative approach with the observation unit of all employees of PT. Sedana Pasifik Servistama and total sampling technique with a total of 110 respondents. The method used is a survey using a questionnaire and descriptive research type explanatory research. This research's data analysis method is the validity test, reliability test, and hypothesis test using SEM (Structural Equation Modeling). The results showed that training and compensation had a positive and significant effect on employee performance. Motivation has a positive and significant effect on employee performance. Motivation can mediate the effect of training on employee performance and motivation to mediate the effect of compensation on employee performance at PT. Servistama Pacific Sedana.
Procesos de deforestación asociados a la minería legal en la Amazonía colombiana
Authors: Nieves-Solarte Fabio Andrés , Ruiz-Fajardo Johan Esteban
Published: July 2021

La Amazonía colombiana es un ecosistema de gran importancia debido a la gran riqueza natural que posee — fauna, flora, oferta hídrica y cultura ancestral—, lo que la convierte en una zona de especial cuidado por parte del Estado. Comprende los departamentos del Amazonas, Caquetá, Cauca, Guainía, Guaviare, Meta, una parte del noroccidente de Nariño, Putumayo y Vichada. El propósito de este trabajo de investigación es evaluar los cambios en la cobertura de bosque en la Amazonía colombiana por actividades mineras legales relacionadas con la extracción de metales, no metales, materiales de construcción y demás concesibles, en el periodo 2002-2014, mediante tecnología SIG —sistemas de información geográfica—. La información cartográfica fue obtenida de entidades gubernamentales como el Sistema de Información Ambiental de Colombia (SIAC) y el Instituto Amazónico de Investigaciones Científicas (Sinchi). Luego se procesaron los datos mediante herramientas de georreferenciación y análisis de atributos. Esta información se cotejó con datos disponibles en el catastro minero, en el cual se verificó el estado actual y el historial de extracción de cada uno de los títulos mineros otorgados entre el 2002 y el 2014. Finalmente, la organización y el análisis de los resultados obtenidos se realizó con herramientas estadísticas que permitieron determinar las zonas con mayor cambio de cobertura de bosque, además de determinar el material de mayor extracción en la zona de estudio y su relación con el anillo poblacional amazónico.
Implementing a Canadian Shared-Care ADHD Program in Beijing: Barriers and Facilitators To Consider Prior To Start-Up.
Authors: Bahraini Sayna , Maisonneuve Alexander , Liu Yirong , Samson André , Ying Qian , Li Fei , Yang Li , Robaey Philippe
Published: July 2021

Background

The ADHD Shared Care Pathways is a program that has been developed in Canada with two main strategies: (a) to implement shared care between general practitioners (GPs) and specialists, and (b) to implement stepped care in which the patient is treated at the most appropriate level of care, depending on complexity or outcome of their illness. The current study aims to identify challenges and facilitators in implementing this program in a Chinese context.
Methods

Two focus groups were conducted using semi-structured interviews with a total of 7 healthcare providers in Beijing. A grounded theory approach using open, axial and selective coding provided three main themes pertaining to the barriers and facilitators faced at: (1) a Social-level from of the perspectives of patients and healthcare providers; (2) at a structural-level related to both internal and external organizational environments; (3) and at the intervention-level.
Results

Results reveal multilayered challenges in implementing an ADHD Shared Care Pathways program for children in China.
Conclusion

Our study highlights the importance of consultation in a new implementation context in order to get a “lay of the land”. By extension, our results demonstrate areas for service development and further research.
Examination of the Illness Representations among Children with T1DM in Relation to Mental Health Factors
Authors: Nagy Beáta Erika , Munkácsi Brigitta , Felszeghy Enikő Noémi , Kenyhercz Flóra , Szőllősi Gergő József , Kovács Karolina Eszter
Published: July 2021

Background: This study aimed to investigate the association of the mental health and diabetes-specific variables with the illness representations in children with type 1 diabetes mellitus (T1DM).Methods: A total of 115 children with T1DM were recruited through the outpatient clinic in Debrecen, Hungary. Our measures were The Pictorial Representation of Illness and Self Measure Drawing Test (PRISM-D), Child Depression Inventory (CDI), Cantril Self-Anchoring Striving Scale, and Self-Rated Health (SRH). Glycosylated haemoglobin (HbAlc) values were obtained from medical reports. Results: We found significant mild correlations amongst the mental health factors. Those children who can be characterized with clinical depressive symptoms drew significantly fewer (r = - 0.253; p < 0.05) circles with significantly less area (r = - 0.253; p < 0.05) at the PRISM-D test. Furthermore, those children who did not draw any important relationships possessed significantly more depressive symptoms than those who drew at least one important person. Those children who were diagnosed at a younger age, reported significantly better health condition (r = 0.215; p= 0.024), and displayed smaller distance (r = -0.261; p= 0.006) between the Self-circle and the Illness-circle. Conclusions: The PRISM-D test provides a more complex picture of the subjective effect of the disease, and thus appears a promising additional tool to analyse the emotional and cognitive representations and the psychological burden of T1DM. Beside the medical treatment of the T1DM-children, the earliest screening and psychological interventions for mental health problems are of high priority.
Characterization of Copper Tailings In Murgul Copper Plant (Artvin/Turkey) And Utilization Potential In Cement Mortar With Nano And Micro Silica
Authors: KARA İlknur BEKEM
Published: July 2021

Due to the increasing demand for copper day by day, copper tailings (CT) are the wastes that mining and human-induced activities caused have become a problem all over the world due to the increasing demand for copper. This study evaluates the effect of using CT together with nano silica (NS) and micro silica (MS) in mortars as a partial substitution for cement on mechanical strength properties. Physical, morphological, chemical, mineralogical thermo gravimetric analyzes of CT have been made. In addition, heavy metal concentrations were determined. The mechanical features of the mortars produced by replacing by weight with different proportions of (5%, 10%, 15%) CT, 2% NS and 10% MS cement were determined. As a result, it has been observed that the sum of SiO2, Al2O3 and Fe2O3 of the CT, which has a mostly crystalline structure, is 91.40% and its ignition loss is 4.04%. An improvement in compressive strength (compared to the reference mixture) was observed with the use of 5% CT. Up to 10% of CT has provided standard compressive strength values in both NS and MS combinations.
Carbon Footprint Evaluation of Hazardous Waste Based Solid Fuel: Application in a Cement Kiln
Authors: Karpan Balasubramaniam , Raman Abdul Aziz Abdul , Rahim Razuana , Aroua Kheireddine Taieb , Buthiyappan Archina
Published: July 2021

This study aimed to evaluate industrial wastes-based solid fuel (IWSF) carbon footprint from the boundary of the cradle-to-grave life cycle. It includes emissions released from the transportation, manufacturing of IWSF, waste disposal, utilization of IWSF in the cement manufacturing plant, and end of life of IWSF. The quantification of total IWSF carbon footprint measures greenhouse gas emissions, carbon dioxide, nitrous oxide, and methane and is expressed as carbon dioxide equivalent (CO2-eq). The CO2-eq emission factors are calculated based on Intergovernmental Panel on Climate Change (IPCC) guideline, and the information used in this study is obtained from the actual operation. The study confirmed that the total carbon footprint of IWSF is approximately 0.17 kg CO2-Eq. MJ-1 energy generated. The results show that the utilization of IWSF at a cement manufacturing plant is the key contributor to carbon footprint, contributing to 94.3% of the total percentage, with a quantitative value of 27,000.7 MT CO2-eq per year IWSF manufacturing stage with 2.6 %. Subsequently, CO2-eq emission reduction initiatives have been implemented by the IWSF manufacturer, able to reduce approximately 333 MT of CO2-eq emission and total cost saving of USD50 000 annually. This study proves that industrial hazardous waste can be a source of fuel with positive economic and environmental returns. Besides, it was noted from the study that while direct combustion of solid-derived fuels can efficiently produce heat, it can also lead to the generation of greenhouse gases during the production and use phases. In summary, to estimate GHG emissions from IWSF production, a Life Cycle Assessment- Carbon Footprint (LCA-CF) should be considered.
Bagging Based Machine Learning Algorithms for Landslide Susceptibility Modeling
Authors: Zhang Tingyu , Fu Quan , Wang Hao , Liu Fangfang , Wang Huanyuan , Han Ling
Published: July 2021

Landslide hazards have attracted increasing public attention over the past decades due to a series of catastrophic consequences of landslide occurrence. Thus, the mitigation and prevention of landslide hazards have been the topical issues. Thereinto, numerous research achievements on landslide susceptibility assessment have been springing up in recent years. In this paper, four benchmark models including best-first decision tree (BFTree), functional tree (FT), support vector machine (SVM) and classification regression tree (CART) and were integrated with bagging strategy. Then these bagging-based models were applied to map regional landslide susceptibility in Jiange County, Sichuan Province, China. Fifteen conditioning factors were employed in establishing landslide susceptibility models, respectively, slope aspect, slope angle, elevation, plan curvature, profile curvature, TWI, SPI, STI, lithology, soil, land use, NDVI, distance to rivers, distance to roads and distance to lineaments. Then utilize correlation attribute evaluation (CAE) method to weigh the contribution of each factor. Finally, the comprehensive performance of various bagging-based models and corresponding benchmark models was evaluated and systematically compared applying receiver operating characteristic curve (ROC) and area under curve (AUC) values. Results demonstrated that bagging-based ensemble models significantly outperformed their corresponding benchmark models with validation dataset. among them the Bag-CART model has the highest AUC value of 0.874, however the AUC value of CART model is only 0.766, which reflected satisfying predictive capacity of integrated models in some degree. The achievements obtained in this study have some reference values for landslides prevention and land resource planning in Jiange County.
miR-4319 Reduces Tumorigenicity of Cervical Cancer Cells by Targeting TUFT1
Authors: Zheng Lijun , Ren Qiongzhen , Zhu Weipei , Tao Xiaomin , Guo Liangsheng
Published: July 2021

Background: In the present study, a new tumor suppressor function of miR-4319 was disclosed in CC. Methods: Up-regulation of miR-4319 suppressed cell viability, proliferation, migration, invasion, and induced cell apoptosis in CC cells were measured by cell transfection, CCK-8, colony formation, EdU, flow cytometer, wound healing, transwell migration and invasion and western blot assays. Moreover, Tuftelin 1 (TUFT1) was verified as a direct target of miR-4319 by binding its 3’-UTR, confirmed by dual-luciferase reporter assay. Result: The expression of miR-4319 was obviously decreased in clinical CC tissues and CC cell lines.TUFT1 was remarkably increased in clinical CC tissues and CC cell lines, and was negatively associated with miR-4319 expression. Furthermore, over-expression of TUFT1 partially restored the effects of miR-4319 mimic on cell viability, proliferation, migration, invasion, and cell apoptosis in CC cells. Conclusion: miR-4319 played an anti-cancer role in the occurrence and development of CC, which might be achieved by targeting TUFT1.
Seroprevalence Canine Survey For Selected Vector-Borne Pathogens of and Its Relationship With Poverty in Metropolitan Pereira, Colombia, 2020*
Authors: Bonilla-Aldana D. Katterine , Gutiérrez-Grajales Erwin J. , Martínez-Arboleda J. Paola , Reina-Mora María Angelica , Trejos-Mendoza Adrián E. , Pérez-Vargas Soffia , Valencia-Mejía Lorenzo , Marín-Arboleda Luisa F. , Osorio-Navia Daniela , Chacón-Peña Mariana , González-Colonia Luz Victoria , Cardona-Ospina Jaime A. , Jiménez-Posada Erika Vanessa , Diaz Andrés , Salazar Jean Carlos , Sierra Manuel , Muñoz Fausto , Zambrano Lysien I. , Ramírez-Vallejo Eduardo , Álvarez Juan Camilo , Jaramillo-Delgado Ingrid Lorena , Pecho-Silva Samuel , Paniz-Mondolfi Alberto , Faccini-Martínez Álvaro A. , Rodriguez-Morales Alfonso J.
Published: July 2021

Background
Tick-borne diseases (TBD) and dirofilariasis are currently not under surveillance in most Latin American countries, and there is a significant lack of studies describing the current situation in most endemic areas, including Colombia. Seroprevalence studies are crucial for understanding the epidemiology of these vector-borne diseases.
Methods
A serosurvey for TBD and dirofilariasis amongst 100 dogs was carried out in the municipality of Pereira, located in the Coffee-Triangle region, Colombia. Samples were tested using a rapid assay test system (SNAP® 4Dx®); based on an enzyme immunoassay technique‚ screening for antibodies to Anaplasma phagocytophilum/platys (sensitivity 99.1%)‚ Borrelia burgdorferi s.l. (98.8%), and Ehrlichia canis/ewingii (96.2%) by using specific antigens, and checking for Dirofilaria immitis antigen based on specific antibodies (99.2%). Bivariate analyses were performed on Stata®14, significant p < 0.05.
Results
Global seroprevalence to the selected vector-borne pathogens was 74% (95%CI 65–83%). The highest seroprevalence was found for Ehrlichia canis/ewingii (74%), followed by Anaplasma phagocytophilum/platys (16%). Seropositivity for Borrelia spp. and Dirofilaria spp. was 0%. All Anaplasma spp. seropositive dogs showed co-detection of Ehrlichia spp. (16%). Seroprevalence was significantly higher among dogs from families of lower socioeconomic status / level (I, 86%), followed by level II (74%), and III (36%) (p = 0.001); no seropositive dogs were found in for levels IV and V (higher socioeconomic status). All dogs exhibiting anorexia (12%) were invariably seropositive (100%) (p = 0.029). Seroprevalence was higher amongst those showing mucocutaneous paleness (95%) compared to those without paleness (68%) (p = 0.013) (OR = 9.3; 95%CI 1.18–72.9). There was high variability in seroprevalence through the studied areas, ranging from 0% (La Libertad Park) up to Combia, Cesar Nader, Las Brisas and Saturno localities (100%) (p = 0.033).
Conclusions
Given the high seroprevalence obtained in an area with documented presence of ticks, there is a potential risk for zoonotic transmission to humans. Further seroprevalence studies in humans are needed to assess the prevalence of infections. Poverty is highly associated with these tick-borne pathogens in Pereira, as shown in the present study.
The Diagnostic Value of Preoperative Hematology Makers in the Classification and Molecular Subtypes of Newly Diagnosed High-Grade Gliomas
Authors: Jia Xin , Zhai Yixuan , Yang Fengdong , Wang Yiming , Wei Shuxin , Wei Xinting
Published: July 2021

Objective

The purpose of our study is to explore the diagnostic value of the single and combined hematological maker for the classification and isocitrate dehydrogenase (IDH)-1/2 mutations molecular subtypes of high-grade gliomas (HGGs).
Methods

A total of 354 newly diagnosed HGGs patients were included in this study. Firstly, we compared the levels of hematology indicators in the classification and molecular subtypes of HGGs. Next, the correlation between the levels of hematology indicators with basic clinical features was analyzed. Finally, the diagnostic value of the single and combined hematology indicator for identifying the classification and molecular subtypes from HGGs was performed.
Results

The level of fibrinogen (FIB) was higher in higher grade gliomas and glioblastoma multiforme IDH wild type (GBM IDH-wt). Nutrition-related indicators such as serum albumin (ALB), albumin/globulin ratio (AGR), and prognostic nutrition index (PNI) were negatively correlated with age, whereas FIB was positively associated with age. Compared with women, men with GBM had significantly higher AGR and lower serum globulin (GLOB). We found that the best single and combined indicator for identifying GBM and GBM IDH-wt from HGGs were FIB [0.595 (0.519–0.672) and 0.615 (0.546–0.684)] and age + FIB [0.712 (0.642–0.783) and 0.726 (0.662–0.791)], respectively.
Conclusions

Preoperative hematological indicators have high diagnostic value for GBM and GBM IDH-wt from HGGs, especially FIB combined age.
Effect of Peridontal Interventions on Characteristics of the Periodontal Microbiota: A Systematic Review and Meta-analysis
Authors: Nath Sonia , Pulikkotil Shaju , Weyrich Laura , Zilm Peter , Kapellas Kostas , Ketagoda Kevin , Jamieson Lisa
Published: July 2021

Background: The aim of the systematic review was to evaluate the effect of periodontal interventions on the diversity and composition of periodontal microbiota assessed by high throughput sequencing (HTS) metagenomics analysis. Methods: An electronic search of all relevant databases was conducted from database inception to May 2021 by two independent reviewers. All clinical trials that evaluated the effect of periodontal interventions on the gingival microbiota through HTS were selected. The measures of alpha diversity was used as primary outcome; richness, Shannon diversity index, and the Chao1 index, whereas relative abundances of bacterial genera was considered as secondary outcome. The Robvis tool was used to assess the risk of bias. Results: Overall, 19 studies were eligible for the systematic review, of which 10 studies were included in the meta-analysis. Periodontal intervention for test group resulted in decrease in Shannon’s diversity, richness and Chao I index as observed from baseline to post-treatment. The most common genera found to increase after periodontal therapy were Rothia, Actinomyces, Streptococcus, Veillonella and Hemophilus and Porphyromonas, Tannerella, Fusobacterium, and Treponema decreased after periodontal therapy. Conclusion: Periodontal interventions may decrease the bacterial diversity and richness and alter the composition of oral microbiota in the short-term. Periodontal microbiota signatures could potentially be used for the assessment of periodontal intervention in the future. Further studies should implement methodological consistency to provide a more quantitative and mechanistic understanding of these approaches.
Comparative Transcriptome and DNA Methylation Analysis in Temperature-Sensitive Genic Male Sterile Wheat BS366
Authors: Liu Yong-jie , Li Dan , Gong Jie , Wang Yong-bo , Chen Zhao-bo , Pang Bin-shuang , Chen Xian-chao , Gao Jian-gang , Yang Wei-bing , Zhang Feng-ting , Tang Yi-miao , Zhao Chang-ping , Gao Shi-qing
Published: July 2021

Background: Known as the prerequisite component for the heterosis breeding system, the male sterile line determined the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, were male sterile under cold condition (12℃ with 12 h daylight) but fertile under normal temperature (20℃ with 12 h daylight). Results: During the meiosis, BS366 was defective in forming tetrad and dyad due to the abnormal cell plate. During the pollen development, unusual vacuolated pollen that cannot accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process like sister chromatid segregation and microtubule-based movement were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold condition. MethylRAD was used for a reduced DNA methylation sequencing for BS366 under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Genes differentially and methylated were mainly involved in cell division. Conclusions: These results indicated that methylation of genes involved in carbon metabolism or fatty acid metabolism would contribute to the male sterility in BS366, which will provide a novel insight into the molecular mechanism of wheat male sterility.
Effect of Gas Holdup on CO2 Biofixation by Spirulina sp. in the 20-liter Airlift Bioreactor
Authors: Zarei Zahra , Malekshahi Peyman , Morowvat Mohammad Hossein , Rahimi Rahbar , Niknezhad Seyyed Vahid
Published: July 2021

The rise of CO2 concentration in the Earth is a major environmental problem, which cause global warming. To solve this issue, several methods have been applied, but among these solutions using microalgae is an eco-friendly and cost-effective way of reducing carbon dioxide, as they can efficiently sequestrate CO2 and produce biomass as valuable products. In this study, hydrodynamic parameters, bubble sizes and carbon dioxide uptake were investigated in an airlift bioreactor. Experiments were studied at two different superficial gas velocities (0.185 and 0.524 cm/s) for Spirulina sp. microalgae into a 20-liter airlift bioreactor to find out the amount of carbon dioxide sequestration and cyanobacterial biomass. The highest efficiency of carbon dioxide removal and maximum dry weight of Spirulina sp. were achieved 55.48% and 0.86 g/L respectively at 5% CO2 (v/v) and superficial velocity of 0.185 cm/s. This experiment was conducted in 7 days, light intensity (2600 lux/m2), temperature (30\(\pm\)2 °C) and a light-dark cycle (12–12), which all were constant. The hydrodynamic parameters studied by Spirulina sp. demonstrated a capability of CO2 sequestration in this airlift photobioreactor.
A Nonreciprocal Angular Selective Absorber with the Special Multilayer Structure
Authors: Wan Baofei , Ma Yu , Zhou Ziwei , Zhang Dan , Zhang Hai-Feng
Published: July 2021

Due to the orderly design of the special anti-reflection structure and the absorption structure, the one-dimensional layered periodic structure has a good impedance matching in a certain frequency band and a large-angle range, providing for the realization of an angular selective absorber (ASA) with a high rectangle coefficient. For the sake of obtaining excellent absorptivity, the indium tin oxide (ITO) film is used, and it also acts as a function of tuning the absorption angle range (AAR) of the ASA by adjusting the plasma frequency. The proportional relationship between the thickness of the dielectric layers is also discussed to satisfy a good absorption function. At the same time, the ASA also possesses productive nonreciprocal performance (NP) and can also be controlled by the plasma frequency. The transfer matrix method is used for numerical simulation. Our special tunable ASA with the NP is relatively rare in previous studies, which can be applied to optical communications and military fields. Furthermore, we hope that the design we proposed can provide new possibilities for the development of the ASAs.
Engaging citizens in the co-production of a health system performance assessment framework: a case study in Ireland
Authors: Fernandes Óscar Brito , Barbazza Erica , Ivanković Damir , Jansen Tessa , Klazinga Niek , Kringos Dionne
Published: July 2021

Background

The launch in 2017 of the Irish 10-year reform programme Sláintecare represents a key commitment in the future of the health system. An important component of the programme was the development of a health system performance assessment (HSPA) framework. In 2019, the Department of Health of Ireland (DoH) and Health Service Executive (HSE) commissioned the technical support of researchers to develop an outcome-oriented HSPA framework, which should reflect the shared priorities of multiple stakeholders, including citizens. This study describes the method applied in the Irish context and reflects on the added value of using a citizen panel in the co-production of an HSPA framework.

Methods

A panel of 15 citizens was convened, recruited by a third-party company using a sampling strategy to achieve a balanced mix representing the Irish society. Panelists received lay-language preparatory materials prior to the meeting. Panelists used a three-color scheme to signal the inclusion and importance of performance measures. An exit questionnaire was administered to understand how participants experienced being part of the panel. The citizen panel was the first in a series of three panels towards the development of the HSPA framework, followed by panels including representatives of the DoH and HSE, and representatives from professional associations and special interest groups.

Results

The citizen panel generated 249 health performance measures ranging across 13 domains. Domains assessed as the most important included people-centeredness, coordination of care, and coverage. Prioritization of domains differed between panels. Citizen panelists shared a similar understanding of what a citizen panel involves and described their experience at the panel as enjoyable, interesting, and informative.

Conclusions

The engagement of citizens early on in the co-production process of the HSPA framework shaped the processes that followed, with the restating of priorities of the citizen panel informing decision-making throughout. Citizen engagement in HSPA development is essential for realizing value-based people-centered health systems and assuring an inclusive process that helps to generate trust and ownership of performance intelligence. Future research could expand on how citizen panels could be further engaged in co-creating mechanisms to assess, monitor, and report on the performance of health care systems.
Wildfire Alters the Linkage Between Total and Available Soil C:N:P Ratios and the Stoichiometric Effects on Fine Root Growth in a Chinese Boreal Larch Forest
Authors: Kong Jianjian , Xiang Xing jia , Yang Jian
Published: July 2021

Background and aimsWildfire is a primary driver of ecosystem functioning, and the fire-induced changes in the cycling and balance of multiple nutrients may influence the response of plant growth to burning. However, the relationships between total and available soil stoichiometry and stoichiometric effects on the growth of fine roots following fire in forests remain unclear.MethodsWe measured the total and available soil C, N and P concentrations, their ratios and fine root biomass (FRB) at an unburned control, 1-year-postfire and 11-year-postfire sites in a Chinese boreal larch forest. The relationships between soil stoichiometry and FRB were analyzed.ResultsWildfire significantly reduced the total and available soil C:N:P ratios and FRB immediately postfire. Eleven years postfire, most indicators recovered to the pre-fire levels except total soil C:P and N:P ratios, and available C:N ratio. Wildfire immediately increased the correlations between total and available soil C:N:P ratios, as well as between FRB and soil C:N:P ratios, but reduced the correlations between FRB and soil nutrient supply. These effects became weaker over time.ConclusionsThe effects of wildfire on biogeochemical processes in boreal ecosystems extend to the relationships between total and available soil stoichiometry. Wildfire strengthens the linkage between fine roots and soil stoichiometry, but weakens the effects of soil nutrient supply in the Great Xing’an Mountains. Therefore, the effects of wildfire on the coupling of soil C, N and P cycling can produce a more complex soil-plant interaction in the postfire early succession stage of boreal larch forest.