bluuebunny/crossref_metadata_2025 huggingface dataset:
Doctor of Science (D.Sc.): Time to move towards Higher Doctorate Degrees
Authors: Meo Sultan Ayoub , Jawaid Shaukat Ali , Naseem Nadia
Published: September 2021

The present most modern and highly advanced 21’st century is the era of science and technology. In human history, universities are the basic birthplace of higher education, research, and innovation and play a significant role in the countries’ performance, prosperity, and economic progress. Worldwide, there is a swift shift in the pattern of biological, environmental, economic, and educational systems. This broader change is rotating around the higher academia and its allied innovative research impact. The leading universities develop a culture and curricula as per need and demand and produce knowledge and skills-based professional graduates. The universities prepare graduates to keep in view their country’s requirements and compete with their peers at international levels.Moreover, worldwide, universities are transforming towards higher doctorate degrees (D.Sc / S.Dc) to provide an elevated helipad to the applicant to compete in this modern and highly advanced era. The higher doctoral degree, D.Sc, is earned 6-8 years after the post Ph.D. The candidates with higher academic titles, professional skills, and innovative research could compete and achieve top-ranked positions worldwide. Many universities worldwide, including the United States of America, the United Kingdom, Australia, and New Zealand, promote D.Sc degree programs in various science disciplines, including medical sciences. This manuscript explores the dynamics of a higher doctorate and its significance, need, and demand in academia to compete globally.

doi: https://doi.org/10.12669/pjms.37.7.5119

How to cite this:Meo SA, Jawaid SA, Naseem N. Doctor of Science (D.Sc.): Time to move towards Higher Doctorate Degrees. Pak J Med Sci. 2021;37(7):---------. doi: https://doi.org/10.12669/pjms.37.7.5119

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ranking medical & dental institutions based on accreditation standards: Using thermometer to measure atmospheric pressure
Authors: Chaudhry Saima
Published: September 2021

doi: https://doi.org/10.12669/pjms.37.7.5229

How to cite this:Chaudhry S. Ranking medical & dental institutions based on accreditation standards: Using thermometer to measure atmospheric pressure. Pak J Med Sci. 2021;37(7):---------. doi: https://doi.org/10.12669/pjms.37.7.5229

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Results of Formation Monitoring Professional Competencies Geography Teachers
Authors: Sabel'nikova-Begashvili N. , Damianova E. , Khudoverdova S.
Published: September 2021

The article deals with the results of diagnostics of the level of formation of professional competencies of geography teachers. In the course of the study professional deficits in the subject, methodological, psychological, pedagogical and communicative training of teachers were revealed, which should be paid special attention to when building individual educational trajectories and professional development system.
Motivation of Students of Petroleum Universities to Exercise Physical Culture
Authors: Zubarev S. , Bragina A. , Aleksandrova N.
Published: September 2021

The article analyzes the motivation to engage in physical culture of students of non-physical culture universities. The statistical conclusions of the results of the questioning of students are presented. Priority and uninteresting motives have been determined, the dynamics of changes in students priorities in the learning process has been investigated. Recommendations for activating students to physical culture and sports are formulated.
Method for Assessing the Complexity of Mathematical Reasoning in Educational Texts (on the Example of a Physics Textbook)
Authors: Mayer Robert
Published: September 2021

The article offers the method for assessing the complexity of mathematical reasoning in various educational texts. It provides for counting the number of mathematical statements (or other elementary judgments) in the conclusions being drawn and taking into account the semantic complexity of the terms denoting the quantities included in the formulas. This allows to calculate the heuristic complexity of the text, depending on the number and complexity of mathematical conclusions, and find the heuristic indicator. To determine the semantic complexity of the entire text and the mathematical reasoning presented in it, a computer is used, which refers to a dictionary-thesaurus – a special file containing terms with an indication of their complexity. This takes into account the volumetric complexity of the formulas, the number of elementary sentences (equations) in mathematical reasoning, as well as the fact that some premises (formulas) are absent, and the pupil must guess before them on his own. Several examples of using the proposed method for analyzing text fragments in physics are considered.
On the Experience of Teaching the Discipline “Engineering and Computer Graphics” Under Conditions of Coronavirus Pandemic and Post-Covid
Authors: Polikarpov Yuriy , Hudyakova L. , Semashko M.
Published: September 2021

In connection with the coronavirus pandemic, all higher education institutions were forced from March 2020 to switch to a distance form of student education and complete the academic year in this format. The current 2020-21 academic year, universities began in a mixed format, but since October, the vast majority of higher education institutions have again returned to the distance form of study. In fact, for a whole year, the faculty of universities taught students in the conditions of distance learning. For most departments of universities, such a transition was a complete surprise. The departments were not ready for this turn of events, and they urgently had to develop methods for distance education of students of the taught educational disciplines. The article describes the experience of teachers of the section "Engineering and Computer Graphics" of the department "Fundamentals of Mechanism and Machine Design" of the UGATU in conducting remote classes in the discipline "Engineering and Computer Graphics" in 2019-20 and 2020-21 academic years.
Difficulties in Mastering Universal Educational Cognitive Actions of Students in the 6th and 8th grade (Based on the Results of Diagnostic Work)
Authors: Ambarcumova E.
Published: September 2021

The author characterizes the problems of sixth-and eighth-graders in mastering universal educational cognitive skills in geography lessons; reveals typical mistakes and the reasons for their admission, describes methodological recommendations for their elimination.
Computing Approximate Equilibria in Weighted Congestion Games via Best-Responses
Authors: Giannakopoulos Yiannis , Noarov Georgy , Schulz Andreas S.
Published: September 2021

We present a deterministic polynomial-time algorithm for computing [Formula: see text]-approximate (pure) Nash equilibria in (proportional sharing) weighted congestion games with polynomial cost functions of degree at most [Formula: see text]. This is an exponential improvement of the approximation factor with respect to the previously best deterministic algorithm. An appealing additional feature of the algorithm is that it only uses best-improvement steps in the actual game, as opposed to the previously best algorithms, that first had to transform the game itself. Our algorithm is an adaptation of the seminal algorithm by Caragiannis at al. [Caragiannis I, Fanelli A, Gravin N, Skopalik A (2011) Efficient computation of approximate pure Nash equilibria in congestion games. Ostrovsky R, ed. Proc. 52nd Annual Symp. Foundations Comput. Sci. (FOCS) (IEEE Computer Society, Los Alamitos, CA), 532–541; Caragiannis I, Fanelli A, Gravin N, Skopalik A (2015) Approximate pure Nash equilibria in weighted congestion games: Existence, efficient computation, and structure. ACM Trans. Econom. Comput. 3(1):2:1–2:32.], but we utilize an approximate potential function directly on the original game instead of an exact one on a modified game. A critical component of our analysis, which is of independent interest, is the derivation of a novel bound of [Formula: see text] for the price of anarchy (PoA) of [Formula: see text]-approximate equilibria in weighted congestion games, where [Formula: see text] is the Lambert-W function. More specifically, we show that this PoA is exactly equal to [Formula: see text], where [Formula: see text] is the unique positive solution of the equation [Formula: see text]. Our upper bound is derived via a smoothness-like argument, and thus holds even for mixed Nash and correlated equilibria, whereas our lower bound is simple enough to apply even to singleton congestion games.
On the Impact of Treatment Restrictions for the Indigent Suffering from a Chronic Disease: The Case of Compassionate Dialysis
Authors: Bountali Olga , Çetinkaya Sila , Ahuja Vishal
Published: September 2021

We analyze a congested healthcare delivery setting resulting from emergency treatment of a chronic disease on a regular basis. A prominent example of the problem of interest is congestion in the emergency room (ER) at a publicly funded safety net hospital resulting from recurrent arrivals of uninsured end-stage renal disease patients needing dialysis (a.k.a. compassionate dialysis). Unfortunately, this is the only treatment option for un/under-funded patients (e.g., undocumented immigrants) with ESRD, and it is available only when the patient’s clinical condition is deemed as life-threatening after a mandatory protocol, including an initial screening assessment in the ER as dictated and communicated by hospital administration and county policy. After the screening assessment, the so-called treatment restrictions are in place, and a certain percentage of patients are sent back home; the ER, thus, serves as a screening stage. The intention here is to control system load and, hence, overcrowding via restricting service (i.e., dialysis) for recurrent arrivals as a result of the chronic nature of the underlying disease. In order to develop a deeper understanding of potential unintended consequences, we model the problem setting as a stylized queueing network with recurrent arrivals and restricted service subject to the mandatory screening assessment in the ER. We obtain analytical expressions of fundamental quantitative metrics related to network characteristics along with more sophisticated performance measures. The performance measures of interest include both traditional and new problem-specific metrics, such as those that are indicative of deterioration in patient welfare because of rejections and treatment delays. We identify cases for which treatment restrictions alone may alleviate or lead to severe congestion and treatment delays, thereby impacting both the system operation and patient welfare. The fundamental insight we offer is centered around the finding that the impact of mandatory protocol on network characteristics as well as traditional and problem-specific performance measures is nontrivial and counterintuitive. However, impact is analytically and/or numerically quantifiable via our approach. Overall, our quantitative results demonstrate that the thinking behind the mandatory protocol is potentially naive. This is because the approach does not necessarily serve its intended purpose of controlling system-load and overcrowding.
Ultrafast machine vision with artificial neural network devices based on a GaN-based micro-LED array
Authors: Wang Zhenpeng , Lin Runze , Qu Daopeng , Cui Xugao , Tian Pengfei
Published: September 2021

GaN-based micro-LED is an emerging display and communication device, which can work as well as a photodetector, enabling possible applications in machine vision. In this work, we measured the characteristics of micro-LED based photodetector experimentally and proposed a feasible simulation of a novel artificial neural network (ANN) device for the first time based on a micro-LED based photodetector array, providing ultrafast imaging (∼133 million bins per second) and a high image recognition rate. The array itself constitutes a neural network, in which the synaptic weights are tunable by the bias voltage. It has the potentials to be integrated with novel machine vision and reconfigurable computing applications, acting as a role of acceleration and similar functionality expansion. Also, the multi-functionality of micro-LED broadens its application potentials of combining ANN with display and communication.
Phase gradient protection of stored spatially multimode perfect optical vortex beams in a diffused rubidium vapor
Authors: Chen Yun , Wang Jinwen , Wang Chengyuan , Zhang Shougang , Cao Mingtao , Franke-Arnold Sonja , Gao Hong , Li Fuli
Published: September 2021

We experimentally investigate the optical storage of perfect optical vortex (POV) and spatially multimode perfect optical vortex (MPOV) beams via electromagnetically induced transparency (EIT) in a hot vapor cell. In particular, we study the role that phase gradients and phase singularities play in reducing the blurring of the retrieved images due to atomic diffusion. Three kinds of manifestations are enumerated to demonstrate such effect. Firstly, the suppression of the ring width broadening is more prominent for POVs with larger orbital angular momentum (OAM). Secondly, the retrieved double-ring MPOV beams’ profiles present regular dark singularity distributions that are related to their vortex charge difference. Thirdly, the storage fidelities of the triple-ring MPOVs are substantially improved by designing line phase singularities between multi-ring MPOVs with the same OAM number but π offset phases between adjacent rings. Our experimental demonstration of MPOV storage opens new opportunities for increasing data capacity in quantum memories by spatial multiplexing, as well as the generation and manipulation of complex optical vortex arrays.
Blockchain-basierte Betriebsprüfung am Beispiel der deutschen Finanzverwaltung
Authors: Benke Alessandro , Müller Robert , Houy Constantin , Fettke Peter
Published: September 2021

Die Blockchain-Technologie bietet vielfältige Anwendungspotenziale in den Bereichen des Steuerrechts und der Finanzverwaltung. Der Beitrag analysiert am Beispiel einer Prüfung von steuerlichen Informationen den Einsatz der Blockchain-Technologie in der deutschen Finanzverwaltung. Für die Überprüfung von steuerlichen Sachverhalten ist die Integrität der steuerlichen Informationen des Steuerpflichtigen sowie deren strukturierte Übermittlung an die Finanzverwaltung entscheidend. Aus diesem Grund wird eine Blockchain-basierte Überprüfung von Buchhaltungsdaten mittels Standard Audit Files und der Referenzierung notwendiger Belege entwickelt. Die zunehmende Automatisierung des Informationsmanagements birgt die Gefahr, dass das Steuergeheimnis des Steuerpflichtigen kompromittiert wird. Die Blockchain-Technologie kann durch die manipulationssichere und unveränderliche Dokumentation von Zugriffen auf die steuerlichen Daten einen Geheimnisschutz gewährleisten. Über ein Blockchain-basiertes Berechtigungsmanagement können Zugriffe überwacht und kontrolliert werden.
The importance of non-pharmaceutical interventions during the COVID-19 vaccine rollout
Authors: Gozzi Nicolò , Bajardi Paolo , Perra Nicola
Published: September 2021

The promise of efficacious vaccines against SARS-CoV-2 is fulfilled and vaccination campaigns have started worldwide. However, the fight against the pandemic is far from over. Here, we propose an age-structured compartmental model to study the interplay of disease transmission, vaccines rollout, and behavioural dynamics. We investigate, via in-silico simulations, individual and societal behavioural changes, possibly induced by the start of the vaccination campaigns, and manifested as a relaxation in the adoption of non-pharmaceutical interventions. We explore different vaccination rollout speeds, prioritization strategies, vaccine efficacy, as well as multiple behavioural responses. We apply our model to six countries worldwide (Egypt, Peru, Serbia, Ukraine, Canada, and Italy), selected to sample diverse socio-demographic and socio-economic contexts. To isolate the effects of age-structures and contacts patterns from the particular pandemic history of each location, we first study the model considering the same hypothetical initial epidemic scenario in all countries. We then calibrate the model using real epidemiological and mobility data for the different countries. Our findings suggest that early relaxation of safe behaviours can jeopardize the benefits brought by the vaccine in the short term: a fast vaccine distribution and policies aimed at keeping high compliance of individual safe behaviours are key to mitigate disease resurgence.
Characteristic changes in EEG spectral powers of patients with opioid-use disorder as compared with those with methamphetamine- and alcohol-use disorders
Authors: Minnerly Christopher , Shokry Ibrahim M. , To William , Callanan John J. , Tao Rui
Published: September 2021

Electroencephalography (EEG) likely reflects activity of cortical neurocircuits, making it an insightful estimation for mental health in patients with substance use disorder (SUD). EEG signals are recorded as sinusoidal waves, containing spectral amplitudes across several frequency bands with high spatio-temporal resolution. Prior work on EEG signal analysis has been made mainly at individual electrodes. These signals can be evaluated from advanced aspects, including sub-regional and hemispheric analyses. Due to limitation of computational techniques, few studies in earlier work could conduct data analyses from these aspects. Therefore, EEG in patients with SUD is not fully understood. In the present retrospective study, spectral powers from a data house containing opioid (OUD), methamphetamine/stimulants (MUD), and alcohol use disorder (AUD) were extracted, and then converted into five distinct topographic data (i.e., electrode-based, cortical subregion-based, left-right hemispheric, anterior-posterior based, and total cortex-based analyses). We found that data conversion and reorganization in the topographic way had an impact on EEG spectral powers in patients with OUD significantly different from those with MUD or AUD. Differential changes were observed from multiple perspectives, including individual electrodes, subregions, hemispheres, anterior-posterior cortices, and across the cortex as a whole. Understanding the differential changes in EEG signals may be useful for future work with machine learning and artificial intelligence (AI), not only for diagnostic but also for prognostic purposes in patients with SUD.
Accelerating whole-heart 3D T2 mapping: Impact of undersampling strategies and reconstruction techniques
Authors: Zhu Dan , Ding Haiyan , Zviman M. Muz , Halperin Henry , Schär Michael , Herzka Daniel A.
Published: September 2021

Purpose

We aim to determine an advantageous approach for the acceleration of high spatial resolution 3D cardiac T2 relaxometry data by comparing the performance of different undersampling patterns and reconstruction methods over a range of acceleration rates.

Methods

Multi-volume 3D high-resolution cardiac images were acquired fully and undersampled retrospectively using 1) optimal CAIPIRINHA and 2) a variable density random (VDR) sampling. Data were reconstructed using 1) multi-volume sensitivity encoding (SENSE), 2) joint-sparsity SENSE and 3) model-based SENSE. Four metrics were calculated on 3 naïve swine and 8 normal human subjects over a whole left-ventricular region of interest: root-mean-square error (RMSE) of image signal intensity, RMSE of T2, the bias of mean T2, and standard deviation (SD) of T2. Fully sampled data and volume-by-volume SENSE with standard equally spaced undersampling were used as references. The Jaccard index calculated from one swine with acute myocardial infarction (MI) was used to demonstrate preservation of segmentation of edematous tissues with elevated T2.

Results

In naïve swine and normal human subjects, all methods had similar performance when the net reduction factor (Rnet) <2.5. VDR sampling with model-based SENSE showed the lowest RMSEs (10.5%-14.2%) and SDs (+1.7–2.4 ms) of T2 when Rnet>2.5, while VDR sampling with the joint-sparsity SENSE had the lowest bias of mean T2 (0.0–1.1ms) when Rnet>3. The RMSEs of parametric T2 values (9.2%-24.6%) were larger than for image signal intensities (5.2%-18.4%). In the swine with MI, VDR sampling with either joint-sparsity or model-based SENSE showed consistently higher Jaccard index for all Rnet (0.71–0.50) than volume-by-volume SENSE (0.68–0.30).

Conclusions

Retrospective exploration of undersampling and reconstruction in 3D whole-heart T2 parametric mapping revealed that maps were more sensitive to undersampling than images, presenting a more stringent limiting factor on Rnet. The combination of VDR sampling patterns with model-based or joint-sparsity SENSE reconstructions were more robust for Rnet>3.
Adherence to community versus facility-based delivery of monthly malaria chemoprevention with dihydroartemisinin-piperaquine for the post-discharge management of severe anemia in Malawian children: A cluster randomized trial
Authors: Nkosi-Gondwe Thandile , Robberstad Bjarne , Mukaka Mavuto , Idro Richard , Opoka Robert O. , Banda Saidon , Kühl Melf-Jakob , O. Ter Kuile Feiko , Blomberg Bjorn , Phiri Kamija S.
Published: September 2021

Background

The provision of post-discharge malaria chemoprevention (PMC) in children recently admitted with severe anemia reduces the risk of death and re-admissions in malaria endemic countries. The main objective of this trial was to identify the most effective method of delivering dihydroartemesinin-piperaquine to children recovering from severe anemia.

Methods

This was a 5-arm, cluster-randomized trial among under-5 children hospitalized with severe anemia at Zomba Central Hospital in Southern Malawi. Children were randomized to receive three day treatment doses of dihydroartemesinin-piperaquine monthly either; 1) in the community without a short text reminder; 2) in the community with a short message reminder; 3) in the community with a community health worker reminder; 4) at the facility without a short text reminder; or 5) at the facility with a short message reminder. The primary outcome measure was adherence to all treatment doses of dihydroartemesinin-piperaquine and this was assessed by pill-counts done by field workers during home visits. Poisson regression was utilized for analysis.

Results

Between March 2016 and October 2018, 1460 clusters were randomized. A total of 667 children were screened and 375 from 329 clusters were eligible and enrolled from the hospital. Adherence was higher in all three community-based compared to the two facility-based delivery (156/221 [70·6%] vs. 78/150 [52·0%], IRR = 1·24,95%CI 1·06–1·44, p = 0·006). This was observed in both the SMS group (IRR = 1·41,1·21–1·64, p<0·001) and in the non-SMS group (IRR = 1·37,1·18–1·61, p<0·001). Although adherence was higher among SMS recipients (98/148 66·2%] vs. non-SMS 82/144 (56·9%), there was no statistical evidence that SMS reminders resulted in greater adherence ([IRR = 1·03,0·88–1·21, p = 0·68). When compared to the facility-based non-SMS arm (control arm), community-based delivery utilizing CHWs resulted in higher adherence [39/76 (51·3%) vs. 54/79 (68·4%), IRR = 1·32, 1·14–1·54, p<0·001].

Interpretation

Community-based delivery of dihydroartemesinin-piperaquine for post-discharge malaria chemoprevention in children recovering from severe anemia resulted in higher adherence compared to facility-based methods.

Trial registration

NCT02721420; ClinicalTrials.gov.
Serum chemerin levels: A potential biomarker of joint inflammation in women with rheumatoid arthritis
Authors: Gonzalez-Ponce Fabiola , Gamez-Nava Jorge I. , Perez-Guerrero Emilio E. , Saldaña-Cruz Ana M. , Vazquez-Villegas Maria L. , Ponce-Guarneros Juan M. , Huerta Miguel , Trujillo Xochitl , Contreras-Haro Betsabe , Rocha-Muñoz Alberto D. , Carrillo-Escalante Maria O. , Sanchez-Rodriguez Esther N. , Gomez-Ramirez Eli E. , Nava-Valdivia Cesar A. , Cardona-Muñoz Ernesto G. , Gonzalez-Lopez Laura ,   on behalf of the Research Group for the Assessment of Prognosis Biomarkers in Autoimmune Disorders
Published: September 2021

Background

Chemerin has a potential role in perpetuating inflammation in autoimmune diseases. Nevertheless, to date, there is no conclusive information on whether high chemerin levels increase the severity of rheumatoid arthritis (RA). Therefore, this study evaluated whether serum chemerin is a biomarker of disease activity in RA patients.

Methods

Study design: cross-sectional. The assessment included clinical and laboratory characteristics, body mass index (BMI) and fat mass. The severity of the disease activity was identified according to the DAS28-CRP index as follows: A) RA with a DAS28-CRP≤2.9 (remission/mild activity) and B) RA with a DAS28-CRP>2.9 (moderate/severe activity). Serum chemerin concentrations were measured by ELISA, and ≥103 ng/mL was considered a high level. Logistic regression analysis was applied to determine whether high chemerin levels were associated with disease activity in RA after adjusting for confounders. Multiple regression analysis was performed to identify variables associated with chemerin levels.

Results

Of 210 RA patients, 89 (42%) subjects had moderate/severe disease activity and had higher serum chemerin levels than patients with low disease activity or remission (86 ± 34 vs 73± 27; p = 0.003). Serum chemerin correlated with the number of swollen joints (r = 0.15; p = 0.03), DAS28-CRP (r = 0.22; p = 0.002), and C-reactive protein levels (r = 0.14; p = 0.04), but no correlation was observed with BMI and fat mass. In the adjusted logistic regression analysis, high chemerin levels (≥103 ng/mL) were associated with an increased risk of moderate/severe disease activity (OR: 2.76, 95% CI 1.35–5.62; p = 0.005). In the multiple regression analysis, after adjusting for potential confounders, serum chemerin levels were associated with higher DAS28-CRP (p = 0.002).

Conclusions

Higher chemerin levels increased the risk of moderate and severe disease activity in RA. These results support the role of chemerin as a marker of inflammation in RA. Follow-up studies will identify if maintaining low chemerin levels can be used as a therapeutic target.
Health workers and Sub Saharan African women’s understanding of equal access to healthcare in Norway
Authors: Lien Inger-Lise
Published: September 2021

This article describes and analyzes conceptions of equal access to healthcare by health workers and Sub Saharan African women living in Norway. The main objective of the study was to find out if there is equal access to healthcare as understood by both the provider and receiver side of healthcare. The two sides have different positions from where to observe and judge the services given, which can give a broader understanding of the healthcare system. Do Sub Saharan African women find healthcare services unjust and discriminating? Do health workers share conceptions of access held by these women? This study used a qualitative fieldwork research design. One hundred interviews were done with health workers and 55 interviews were done with Somali, Gambian and Eritrean women who all had experienced female genital mutilation/cutting (FGM/C). The study found a mismatch in the conceptions of access to healthcare between health workers and the women. Health workers did not believe there was equal access to healthcare and were critical of how the system functioned, whereas the women trusted the system and believed there was equal access. However, both sides had corresponding views on the following challenges facing the healthcare system: little time available to identify symptoms, difficulties in navigating the system, difficulties in getting referrals, and some negative adjudication by some health workers. Bourdieu’s concepts of field, habitus and hysteresis, and candidacy theory were used to analyze the collected data. It was concluded that health workers and the women based their experiences of healthcare on differing cultural frames and expectations. The women seemed to base their assessments of healthcare on previous experiences from their home country, while health workers based their understanding from experiences within the system.
Testing for goodness rather than lack of fit of continuous probability distributions
Authors: Wellek Stefan
Published: September 2021

The vast majority of testing procedures presented in the literature as goodness-of-fit tests fail to accomplish what the term is promising. Actually, a significant result of such a test indicates that the true distribution underlying the data differs substantially from the assumed model, whereas the true objective is usually to establish that the model fits the data sufficiently well. Meeting that objective requires to carry out a testing procedure for a problem in which the statement that the deviations between model and true distribution are small, plays the role of the alternative hypothesis. Testing procedures of this kind, for which the term tests for equivalence has been coined in statistical usage, are available for establishing goodness-of-fit of discrete distributions. We show how this methodology can be extended to settings where interest is in establishing goodness-of-fit of distributions of the continuous type.
Medial prefrontal area reductions, altered expressions of cholecystokinin, parvalbumin, and activating transcription factor 4 in the corticolimbic system, and altered emotional behavior in a progressive rat model of type 2 diabetes
Authors: Ochi Ryosuke , Fujita Naoto , Goto Natsuki , Takaishi Kaho , Oshima Takaya , Nguyen Son Tien , Nishijo Hisao , Urakawa Susumu
Published: September 2021

Metabolic disorders are associated with a higher risk of psychiatric disorders. We previously reported that 20-week-old Otsuka Long-Evans Tokushima fatty (OLETF) rats, a model of progressive type 2 diabetes, showed increased anxiety-like behavior and regional area reductions and increased cholecystokinin-positive neurons in the corticolimbic system. However, in which stages of diabetes these alterations in OLETF rats occur remains unclear. We aimed to investigate anxiety-like behavior and its possible mechanisms at different stages of type 2 diabetes in OLETF rats. Eight- and 30-week-old OLETF rats were used as diabetic animal models at the prediabetic and progressive stages of type 2 diabetes respectively, and age-matched Long-Evans Tokushima Otsuka rats served as non-diabetic controls. In the open-field test, OLETF rats showed less locomotion in the center zone and longer latency to leave the center zone at 8 and 30 weeks old, respectively. The areas of the medial prefrontal cortex were smaller in the OLETF rats, regardless of age. The densities of cholecystokinin-positive neurons in OLETF rats were higher in the lateral and basolateral amygdala only at 8 weeks old and in the anterior cingulate and infralimbic cortices and hippocampal cornu ammonis area 3 at both ages. The densities of parvalbumin-positive neurons of OLETF rats were lower in the cornu ammonis area 2 at 8 weeks old and in the prelimbic and infralimbic cortices at both ages. No apoptotic cell death was detected in OLETF rats, but the percentage of neurons co-expressing activating transcription factor 4 and cholecystokinin and parvalbumin was higher in OLETF rats at both ages in the anterior cingulate cortex and basolateral amygdala, respectively. These results suggest that altered emotional behavior and related neurological changes in the corticolimbic system are already present in the prediabetic stage of OLETF rats.
iPLA2-VIA is required for healthy aging of neurons, muscle, and the female germline in Drosophila melanogaster
Authors: Banerjee Surya Jyoti , Schonbrun Adina , Eizadshenass Sogol , Benji Shimshon , Cantor Yaakov Tzvi , Eliach Liam , Lubin Matthew , Narrowe Zev , Purow Jeremy , Shulman Benjamin , Wiener Leib , Steinhauer Josefa
Published: September 2021

Neurodegenerative disease (ND) is a growing health burden worldwide, but its causes and treatments remain elusive. Although most cases of ND are sporadic, rare familial cases have been attributed to single genes, which can be investigated in animal models. We have generated a new mutation in the calcium-independent phospholipase A2(iPLA2) VIA geneCG6718, theDrosophila melanogasterortholog of humanPLA2G6/PARK14, mutations in which cause a suite of NDs collectively calledPLA2G6-associated neurodegeneration (PLAN). Our mutants display age-related loss of climbing ability, a symptom of neurodegeneration in flies. Although phospholipase activity commonly is presumed to underlie iPLA2-VIA function, locomotor decline in our mutants is rescued by a transgene carrying a serine-to-alanine mutation in the catalytic residue, suggesting that important functional aspects are independent of phospholipase activity. Additionally, we find that iPLA2-VIA knockdown in either muscle or neurons phenocopies locomotor decline with age, demonstrating its necessity in both neuronal and non-neuronal tissues. Furthermore, RNA in situ hybridization shows high endogenousiPLA2-VIAmRNA expression in adult germ cells, and transgenic HA-tagged iPLA2-VIA colocalizes with mitochondria there. Mutant males are fertile with normal spermatogenesis, while fertility is reduced in mutant females. Mutant female germ cells display age-related mitochondrial aggregation, loss of mitochondrial potential, and elevated cell death. These results suggest that iPLA2-VIA is critical for mitochondrial integrity in theDrosophilafemale germline, which may provide a novel context to investigate its functions with parallels to PLAN.
Clinical outcomes of salvage treatment in lymph node-positive prostate cancer patients after radical prostatectomy
Authors: Kim Dowook , Kim Dong-Yun , Kim Jae-Sung , Hong Sung Kyu , Byun Seok-Soo , Lee Sang Eun
Published: September 2021

Introduction

The optimal salvage treatment strategies for lymph node-positive (LNP) patients after radical surgery have not been clearly defined in prostate cancer with biochemical recurrence or persistence of elevated prostate-specific antigen (PSA). In this study, we compared the clinical outcomes of two different salvage treatments, androgen deprivation therapy (ADT) alone versus ADT with radiotherapy (RT). We also investigated prognostic factors that could support the use of ADT with RT in LNP prostate cancer.

Materials and methods

We retrospectively reviewed 94 LNP prostate cancer patients who underwent radical prostatectomy (RP) followed by salvage treatment between 2004 and 2018. Salvage treatments involved either ADT alone or ADT with RT according to the clinical judgment of the physician. We analyzed clinicopathological and treatment factors related to 2nd biochemical failure (2nd BCF), clinical progression (CP), and progression-free survival (PFS). The cumulative failure after salvage treatment was defined as including both 2nd BCF and CP.

Results

The median duration of follow-up was 55 months (interquartile range, 35–97 months). Thirty-seven (39.4%) patients were treated with ADT alone, and 57 patients (60.6%) were treated with a combination of ADT with RT. During follow-up period, the incidence of failure after salvage treatment in the ADT alone group and the combined treatment group was 89.2% and 45.6%, respectively (HR, 22.4; 95% CI 5.43–92.1; P < 0.001). The combination of ADT with RT was associated with better 2nd BCF and PFS than ADT alone (P = 0.007 and P = 0.015, respectively). In multivariate analyses, number of positive LN ≥ 2 and PSA nadir ≥ 0.005 ng/ml after RP were associated with poor 2nd BCF, CP, and PFS after salvage treatment. Salvage by combined ADT plus RT showed better 2nd BCF and PFS than ADT alone. Specifically, patients with number of positive LN ≥ 2 or PSA nadir ≥ 0.005 ng/ml after RP showed better 2nd BCF (P = 0.004) or PFS (P = 0.011) when treated with ADT plus RT rather than ADT alone.

Conclusions

In patients with LNP prostate cancer, salvage ADT plus RT improved 2nd BCF and PFS compared to ADT alone. In particular, when the patients had more than two positive lymph nodes or PSA nadir ≥ 0.005 ng/ml after RP, ADT with RT seems to be a more beneficial salvage treatment resulting in better 2nd BCF and PFS.
Preparations of Dutch emergency departments for the COVID-19 pandemic: A questionnaire-based study
Authors: O’Connor Rory D. , Barten Dennis G. , Latten Gideon H. P.
Published: September 2021

Background

The onset of the COVID-19 pandemic was characterized by rapid increases in Emergency department (ED) patient visits. EDs required an appropriate transformation. The main challenges were: adapting capacity to respond to surges in the number of patient visits, protection of high risk (frontline) staff and the segregation of suspect-COVID-19 patients. To date, only a few studies have assessed the nation-wide response of EDs to the COVID-19 pandemic. This study was designed to review the preparations of Dutch EDs during the initial phase of this public health crisis.

Methods

The study was designed as a nation-wide, cross-sectional, questionnaire-based study of Dutch hospital organizations having one or more EDs. One respondent completed the questionnaire for each hospital. The questionnaire was conducted between the first and the second COVID-19 wave in the Netherlands. It contained close-ended and open-ended questions on changes in ED infrastructure, ED workforce adaptions and the role of emergency physicians (EPs) in each hospital crisis management team.

Results

The questionnaire was completed by 58 respondents. This represented 80% of the total number of EDs. All respondents had made preparations in anticipation of a COVID-19 patient surge. Treatment capacity was expanded in 70% of EDs, with a median increase of 49% (IQR 33–73%). Suspect-COVID-19 was segregated from non-COVID-19 patients in 89% of EDs. Alternative locations (such as outpatient departments) were more often used to assess non-COVID-19 patients, than for suspect-COVID-19 patients. Staff was expanded in 82% of EDs. This largely concerned nursing staff. A formal role for Emergency Physicians (EPs) in the hospital’s crisis management team was reported by 94% of hospital organizations employing EPs.

Conclusion

All Dutch EDs responded to the COVID-19 pandemic in a very short time span despite much uncertainty. Preparations predominantly concerned expansion of treatment capacity and segregation of COVID-19 ED care. EPs played a prominent role, both in direct COVID-19 care and in the hospital crises management team. It is vital for EDs to adapt to community needs swiftly. The ability of EDs to respond to the pandemic varied considerably.
An improved efficient anonymous authentication with conditional privacy-preserving scheme for VANETs
Authors: Cahyadi Eko Fajar , Hwang Min-Shiang
Published: September 2021

The study of security and privacy in vehicular ad hoc networks (VANETs) has become a hot topic that is wide open to discussion. As the quintessence of this aspect, authentication schemes deployed in VANETs play a substantial role in providing secure communication among vehicles and the surrounding infrastructures. Many researchers have proposed a variety of schemes related to information verification and computation efficiency in VANETs. In 2018, Kazemi et al. proposed an evaluation and improvement work towards Azees et al.’s efficient anonymous authentication with conditional privacy-preserving (EAAP) scheme for VANETs. They claimed that the EAAP suffered from replaying attacks, impersonation attacks, modification attacks, and cannot provide unlinkability. However, we also found out if Kazemi et al.’s scheme suffered from the unlinkability issue that leads to a forgery attack. An adversary can link two or more messages sent by the same user by applying Euclid’s algorithm and derives the user’s authentication key. To remedy the issue, in this paper, we proposed an improvement by encrypting the message using a shared secret key between sender and receiver and apply a Nonce in the final message to guarantee the unlinkability between disseminated messages.
Screening plans for SARS-CoV-2 based on sampling and rotation: An example in a European school setting
Authors: Baccini Michela , Cereda Giulia
Published: September 2021

Screening plans for prevention and containment of SARS-CoV-2 infection should take into account the epidemic context, the fact that undetected infected individuals may transmit the disease and that the infection spreads through outbreaks, creating clusters in the population. In this paper, we compare through simulations the performance of six screening plans based on poorly sensitive individual tests, in detecting infection outbreaks at the level of single classes in a typical European school context. The performance evaluation is done by simulating different epidemic dynamics within the class during the four weeks following the day of the initial infection. The plans have different costs in terms of number of individual tests required for the screening and are based on recurrent evaluations on all students or subgroups of students in rotation. Especially in scenarios where the rate of contagion is high, at an equal cost, testing half of the class in rotation every week appears to be better in terms of sensitivity than testing all students every two weeks. Similarly, testing one-fourth of the students every week is comparable with testing all students every two weeks, despite the first one is a much cheaper strategy. In conclusion, we show that in the presence of natural clusters in the population, testing subgroups of individuals belonging to the same cluster in rotation may have a better performance than testing all the individuals less frequently. The proposed simulations approach can be extended to evaluate more complex screening plans than those presented in the paper.
Impact of short-term change of adiposity on risk of high blood pressure in children: Results from a follow-up study in China
Authors: Yang Yi-de , Xie Ming , Zeng Yuan , Yuan Shuqian , Tang Haokai , Dong Yanhui , Zou Zhiyong , Dong Bin , Wang Zhenghe , Ye Xiangli , Hong Xiuqin , Xiao Qiu , Ma Jun
Published: September 2021

This study aimed to examine the impact of short-term adiposity change on risk of high blood pressure (HBP), and to assess the low limit range of body mass index (BMI) and waist-to-height ratio (WHtR) reduction proposed to decrease the HBP risk in children. Children were longitudinally surveyed at baseline and after a short-term follow-up. General obesity (GOB) is categorized by age and gender-specific BMI cut-off points, abdominal obesity (AOB) by WHtR. Logistic regression model was used to estimate relations between adiposity change and HBP risk with adjustment of covariates. A total of 28,288 children (median of baseline age:10 years) were involved with follow-up of 6.88±1.20 months. After the follow-up, 9.4% of the children had persistent general obesity (GOB), 2.8% converted from GOB to non-GOB, 0.9% had newly developed GOB. When compared with children remained non-GOB, children with continuous GOB status, newly developed GOB, converting from GOB to non-GOB had 5.03-fold (95%CI: 4.32~5.86), 3.35-fold (95%CI: 1.99~5.65), 2.72-fold (2.03~3.63) HBP risk, respectively. Similar findings were observed for abdominal obesity (AOB). Reduction of 0.21–0.88 kg/m2of baseline BMI (0.86–3.59%) or 0.009–0.024 of baseline WHtR (1.66–4.42%) in GOB or AOB children, respectively, was associated with significant decrease in HBP risk. Children with persistent obesity, newly developed obesity, or converting from obese to non-obese had significantly higher HBP risk. For children with GOB or AOB, reduction of <3.6% in BMI or <4.5% in WHtR could decrease the HBP risk.
Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)
Authors: Watanabe Yugo , Spangenberg German C. , Shinozuka Hiroshi
Published: September 2021

Recent studies have revealed presence of fungus-originated genes in genomes of cool-season grasses, suggesting occurrence of multiple ancestral gene transfer events between the two distant lineages. The current article describes identification of glucanase-like and monooxygenase-like genes from creeping bent grass, as lateral gene transfer candidates. An in silico analysis suggested presence of the glucanase-like gene in Agrostis, Deyeuxia, and Polypogon genera, but not in other species belonging to the clade 1 of the Poeae tribe. Similarly, the monooxygenase-like gene was confined to Agrostis and Deyeuxia genera. A consistent result was obtained from PCR-based screening. The glucanase-like gene was revealed to be ubiquitously expressed in young seedlings of creeping bent grass. Although expression of the monooxygenase-like gene was suggested in plant tissues, the levels were considerably lower than those of the glucanase-like gene. A phylogenetic analysis revealed close relationships of the two genes between the corresponding genes in fungal endophyte species of the Epichloë genus, suggesting that the genes originated from the Epichloë lineage.
Dengue pre-vaccination screening test evaluation for the use of dengue vaccine in an endemic area
Authors: Limothai Umaporn , Tachaboon Sasipha , Dinhuzen Janejira , Hunsawong Taweewun , Ong-ajchaowlerd Prapapun , Thaisomboonsuk Butsaya , Fernandez Stefan , Trongkamolchai Supachoke , Wanpaisitkul Mananya , Chulapornsiri Chatchai , Tiawilai Anongrat , Tiawilai Thawat , Tantawichien Terapong , Thisyakorn Usa , Srisawat Nattachai
Published: September 2021

Background

The dengue vaccine (Dengvaxia) is only recommended for individuals with prior dengue infection (PDI). This study aimed to perform a serosurvey to inform decision-making for vaccine introduction and identify appropriate target populations. We also evaluated the performance of the serological tests using plaque reduction neutralization test (PRNT) as a reference test in identifying PDI to determine suitability for pre-vaccination screening.

Methods

We enrolled 115 healthy individuals between 10 and 22 years of age living in the Ratchaburi province of Thailand. The serum samples were tested by PRNT to measure the prevalence and concentration of serotype-specific neutralizing antibodies. The performance of the IgG rapid diagnostic test (RDT, SD Bioline, Korea) and IgG enzyme-linked immunosorbent assay (ELISA, EUROIMMUN, Germany) in identifying PDI were evaluated by using PRNT as a reference method.

Results

Ninety-four (81.7%) individuals neutralized one or more dengue serotypes at a titer threshold greater than or equal to 10. Multitypic profiles were observed in 70.4% of the samples which increased to 91.9% in subjects aged 19–22. Among monotypic samples, the highest proportion was reactive against DENV-1 followed by DENV-2, DENV-3, and DENV-4. The highest anti-dengue antibody titers were recorded against DENV-1 and increased with age to a geometric mean NT50 titer (GMT) of 188.6 in the 19–22 age group. While both RDT and ELISA exhibited 100% specificity, RDT demonstrated low sensitivity (35%) with ELISA displaying much greater sensitivity (87%).

Conclusions

Almost 80% of adolescents and youth in Ratchaburi province had already been exposed to one or more of the dengue virus serotypes. The dengue IgG RDT displayed low sensitivity and is likely not be suitable for dengue pre-vaccination screening. These results support the use of IgG ELISA test for dengue vaccination in endemic areas.