bluuebunny/crossref_metadata_2025 huggingface dataset:
Calculation of Semiconductor Power Losses of a Three-Phase Quasi-Z-Source Inverter
Authors: Grgić Ivan , Vukadinović Dinko , Bašić Mateo , Bubalo Matija
Published: October 2020

This paper presents two novel algorithms for the calculation of semiconductor losses of a three-phase quasi-Z-source inverter (qZSI). The conduction and switching losses are calculated based on the output current-voltage characteristics and switching characteristics, respectively, which are provided by the semiconductor device manufacturer. The considered inverter has been operated in a stand-alone operation mode, whereby the sinusoidal pulse width modulation (SPWM) with injected 3rd harmonic has been implemented. The proposed algorithms calculate the losses of the insulated gate bipolar transistors (IGBTs) and the free-wheeling diodes in the inverter bridge, as well as the losses of the impedance network diode. The first considered algorithm requires the mean value of the inverter input voltage, the mean value of the impedance network inductor current, the peak value of the phase current, the modulation index, the duty cycle, and the phase angle between the fundamental output phase current and voltage. Its implementation is feasible only for the Z-source-related topologies with the SPWM. The second considered algorithm requires the instantaneous values of the inverter input voltage, the impedance network diode current, the impedance network inductor current, the phase current, and the duty cycle. However, it does not impose any limitations regarding the inverter topology or switching modulation strategy. The semiconductor losses calculated by the proposed algorithms were compared with the experimentally determined losses. Based on the comparison, the correction factor for the IGBT switching energies was determined so the errors of both the algorithms were reduced to less than 12%.
Varying Levels of Genetic Control and Phenotypic Plasticity in Timing of Bud Burst, Flower Opening, Leaf Senescence and Leaf Fall in Two Common Gardens of Prunus padus L.
Authors: Vander Mijnsbrugge Kristine , Moreels Stefaan
Published: October 2020

Several phenological phases mark the seasonal growth pattern in temperate woody perennials. To gain further insight into the way these phases react on an altering growth environment, we tested whether vegetative and reproductive phenophases in a shrub species respond differentially among different genetic entities and between two different planting sites. We scored leaf bud burst, flower opening, leaf senescence and leaf fall on 267 ramets of Prunus padus L. belonging to 53 genotypes that were sampled in 9 local populations, and that were planted in 2 common gardens in the northern part of Belgium. The data were processed with cumulative logistic regression. The contribution of genetic and non-genetic components to the total variability varied between the four studied seasonal phenophases. The timing of flower opening displayed the smallest relative amount of intragenotypic variance (between ramets), suggesting a stronger genetic control and a lesser need at the individual plant level for plastic fine tuning to the micro-environment. In addition, whereas leaf bud burst showed the highest relative variance at the interpopulation level among all phenophases, probably at least partly attributable to local adaptation, flower opening displayed the highest intergenotypic variance, which may have been promoted more by assortative mating. Spring phenophases were strongly correlated (r = 0.89) as well as the autumnal phenophases (r = 0.72). Flower opening was not correlated with the autumnal phenophases. Timing of leaf bud burst and leaf senescence were negatively correlated, demonstrating that the length of the growing season enlarged or diminished among the studied genotypes. Although the two planting sites were only 24 km apart, all phenophases were advanced at the less exposed site, indicating a phenotypic plastic response. Together, our results suggest that in P. padus, flowering is less sensitive to environmental variation than leaf bud break and may show a lesser impact of a changing environment on this reproductive phenophase.
In Vitro Interactions between Eutypella parasitica and Some Frequently Isolated Fungi from the Wood of the Dead Branches of Young Sycamore Maple (Acer pseudoplatanus)
Authors: Brglez Ana , Piškur Barbara , Ogris Nikica
Published: October 2020

The ten most frequently isolated fungi from the wood of the dead branches of Acer pseudoplatanus L. were tested in dual cultures to evaluate their in vitro antagonistic activity against Eutypella parasitica R.W. Davidson and R.C. Lorenz, the causative agent of a destructive disease of maples in Europe and North America. The tested fungi, treated also as challenge isolates, were Diaporthe sp., Eutypa sp., Eu. maura, E. parasitica, Fusarium avenaceum, Neocucurbitaria acerina, Neonectria sp., Peniophora incarnata, Petrakia irregularis, and Phomopsis pustulata. The antagonistic ability of each challenge isolate was evaluated by calculating an index of antagonism (AI) based on the interaction type in the dual cultures. The results of competition between the fungal isolates were quantified after re-isolations from the interaction zone (s). The dual cultures revealed two main types of competitive interactions: Deadlock, consisting of mutual inhibition after mycelial contact or at a distance, and replacement, reflecting in the inhibition of E. parasitica, followed by partial overgrowth by the replacing fungus. Statistical analysis showed significant differences in average AI and s of challenge isolates between different dual culture assays. Based on the results of the antagonism index, Eutypa sp., Eu. maura, Neonectria sp., and P. incarnata had the highest inhibitory effect on E. parasitica growth and were recognized as the most promising candidates for further biocontrol studies of E. parasitica. The mycelium of E. parasitica at the interaction zones remained mostly viable, except in dual cultures with Eutypa sp., F. avenaceum, and Neonectria sp., where re-isolations did not yield any colony of the E. parasitica isolate. Based on the results, we assume that E. parasitica is a weak competitor, which invests less energy in direct mycelial competition. We discuss the potential of the observed antagonists as a possible biocontrol of Eutypella canker of maple. Nevertheless, additional experiments should be performed for a solid conclusion about competitive ability of E. parasitica and usefulness of antagonists as biocontrol.
Valorization of European Cranberry Bush (Viburnum opulus L.) Berry Pomace Extracts Isolated with Pressurized Ethanol and Water by Assessing Their Phytochemical Composition, Antioxidant, and Antiproliferative Activities
Authors: Dienaitė Lijana , Pukalskienė Milda , Pereira Carolina V. , Matias Ana A. , Venskutonis Petras Rimantas
Published: October 2020

Defatted by supercritical CO2, Viburnum opulus berry pomace (VOP) was subjected to consecutive extraction with pressurized ethanol (E) and water (W) and yielded 23% of VOP-E and 8% of VOP-W, respectively. The major phytochemical groups covering 42 identified and quantified constituents in VOP extracts were organic and phenolic acids, iridoids, quercetin and (epi)catechin derivatives, flavalignans, procyanidins, and anthocyanins. The on-line HPLC-DPPH•-scavenging assay revealed the presence of numerous antioxidants. VOP-E had a higher total phenolic content, was a stronger antioxidant (equivalent to 0.77, 0.42, and 0.17 g trolox/g in oxygen radical absorbance capacity (ORAC), ABTS, and DPPH assays, respectively), and recovered the major part of phenolics from the pomace; however, both extracts demonstrated similar antioxidant activity in the cellular assay. VOP-E inhibited HT29 cancer cells at non-cytotoxic concentrations. The results of this study revealed that VOP contains valuable phytochemicals possessing antioxidant and antiproliferative activities. Consequently, extracts from VOP substances may be of interest in developing functional ingredients for healthy foods, nutraceuticals, and cosmeceuticals.
Regulatory Effect of Methylation of the Porcine AQP3 Gene Promoter Region on Its Expression Level and Porcine Epidemic Diarrhea Virus Resistance
Authors: Wu Jia-Yun , Wang Fang , Wu Zheng-Chang , Wu Sheng-Long , Bao Wen-Bin
Published: October 2020

As an important carrier for intestinal secretion and water absorption, aquaporin 3 (AQP3) is closely related to diarrhea. In this study, we investigated the mechanisms of AQP3 gene expression regulation in porcine epidemic diarrhea virus (PEDV)-induced diarrhea confirmed by PCR amplification and sequencing. Evaluation of intestinal pathology showed that diarrhea caused by PEDV infection destroyed the intestinal barrier of piglets. qPCR analysis showed that AQP3 expression in the small intestine of PEDV-infected piglets was extremely significantly decreased. qPCR and Bisulfite sequencing PCR revealed an increase in the methylation levels of both CpG islands in the AQP3 promoter region in the jejunum of PEDV-infected piglets. The methylation of mC-20 and mC-10 sites within the two CpG islands showed a significant negative correlation with AQP3 expression. Chromatin Co-Immunoprecipitation (ChIP)-PCR showed that the Sp1 transcription factor was bound to the AQP3 promoter region containing these two CpG sites. AQP3 expression was also extremely significantly reduced in Sp1-inhibited IPEC-J2 cells, indicating that abnormal methylation at the mC-20 site of CpG1 and the mC-10 site of CpG2 reduces its expression in PEDV-infected piglet jejunum by inhibiting the binding of Sp1 to the AQP3 promoter. These findings provide a theoretical basis for further functional studies of porcine AQP3.
BMPR2 Promoter Variants Effect Gene Expression in Pulmonary Arterial Hypertension Patients
Authors: Song Jie , Hinderhofer Katrin , Kaufmann Lilian T. , Benjamin Nicola , Fischer Christine , Grünig Ekkehard , Eichstaedt Christina A.
Published: October 2020

Pathogenic variants have been identified in 85% of heritable pulmonary arterial hypertension (PAH) patients. These variants were mainly located in the bone morphogenetic protein receptor 2 (BMPR2) gene. However, the penetrance of BMPR2 variants was reduced leading to a disease manifestation in only 30% of carriers. In these PAH patients, further modifiers such as additional pathogenic BMPR2 promoter variants could contribute to disease manifestation. Therefore, the aim of this study was to identify BMPR2 promoter variants in PAH patients and to analyze their transcriptional effect on gene expression and disease manifestation. BMPR2 promoter variants were identified in PAH patients and cloned into plasmids. These were transfected into human pulmonary artery smooth muscle cells to determine their respective transcriptional activity. Nine different BMPR2 promoter variants were identified in seven PAH families and three idiopathic PAH patients. Seven of the variants (c.-575A>T, c.-586dupT, c.-910C>T, c.-930_-928dupGGC, c.-933_-928dupGGCGGC, c.-930_-928delGGC and c.-1141C>T) led to a significantly decreased transcriptional activity. This study identified novel BMPR2 promoter variants which may affect BMPR2 gene expression in PAH patients. They could contribute to disease manifestations at least in some families. Further studies are needed to investigate the frequency of BMPR2 promoter variants and their impact on penetrance and disease manifestation.
Detargeting Lentiviral-Mediated CFTR Expression in Airway Basal Cells Using miR-106b
Authors: Choi Soon H. , Reeves Rosie E. , Romano Ibarra Guillermo S. , Lynch Thomas J. , Shahin Weam S. , Feng Zehua , Gasser Grace N. , Winter Michael C. , Evans T. Idil Apak , Liu Xiaoming , Luo Meihui , Zhang Yulong , Stoltz David A. , Devor Eric J. , Yan Ziying , Engelhardt John F.
Published: October 2020

Lentiviral-mediated integration of a CFTR transgene cassette into airway basal cells is a strategy being considered for cystic fibrosis (CF) cell-based therapies. However, CFTR expression is highly regulated in differentiated airway cell types and a subset of intermediate basal cells destined to differentiate. Since basal stem cells typically do not express CFTR, suppressing the CFTR expression from the lentiviral vector in airway basal cells may be beneficial for maintaining their proliferative capacity and multipotency. We identified miR-106b as highly expressed in proliferating airway basal cells and extinguished in differentiated columnar cells. Herein, we developed lentiviral vectors with the miR-106b-target sequence (miRT) to both study miR-106b regulation during basal cell differentiation and detarget CFTR expression in basal cells. Given that miR-106b is expressed in the 293T cells used for viral production, obstacles of viral genome integrity and titers were overcome by creating a 293T-B2 cell line that inducibly expresses the RNAi suppressor B2 protein from flock house virus. While miR-106b vectors effectively detargeted reporter gene expression in proliferating basal cells and following differentiation in the air–liquid interface and organoid cultures, the CFTR-miRT vector produced significantly less CFTR-mediated current than the non-miR-targeted CFTR vector following transduction and differentiation of CF basal cells. These findings suggest that miR-106b is expressed in certain airway cell types that contribute to the majority of CFTR anion transport in airway epithelium.
Genetic Resources of Olea europaea L. in the Garda Trentino Olive Groves Revealed by Ancient Trees Genotyping and Parentage Analysis of Drupe Embryos
Authors: Moreno-Sanz Paula , Lombardo Luca , Lorenzi Silvia , Michelotti Franco , Grando Maria Stella
Published: October 2020

The area of the Garda Lake within the Trentino province (north of Italy) is the northernmost part of Europe where the Mediterranean species Olea europaea L. is traditionally cultivated. ‘Casaliva’ is claimed as the main variety traditionally grown in the Garda Trentino area (GT) from which a world renowned niche extra virgin olive oil is produced. Since a dominant presence of ‘Casaliva’ would link the fruit set success and yield to a self-pollination compatibility system, a deep genetic survey of the olive tree population in the GT has been performed with the aim of establishing the actual varietal composition and of understanding from which pollen donor the ‘Casaliva’ olives originate. Forty-four different genetic profiles were observed among the 205 leaf samples collected from 106 ancient trees through the analysis of 20 nuclear microsatellite markers. The varietal composition in modern orchards was also explored and the vast majority of the additional 151 trees analyzed showed the same genotype as the ancient accessions of ‘Casaliva’. The results support the long historical link of ‘Casaliva’ with the GT and, besides a high varietal homogeneity, they also revealed the presence of olive genetic resources essential to fruit production. In fact, the parentage analysis of 550 embryos from drupes of ‘Casaliva’ evidenced that a cross-fertilization system is favored and a list of candidate cultivars most suitable as local pollinizers of ‘Casaliva’ was identified.
Reconstructing Boulder Deposition Histories: Extreme Wave Signatures on a Complex Rocky Shoreline of Malta
Authors: Mottershead Derek. N. , Soar Philip J. , Bray Malcolm J. , Hastewell Linley J.
Published: October 2020

The Żonqor coastline, southeast Malta, displays an exceptional range of geomorphic signatures of extreme coastal events. This paper brings together evidence acquired from a field survey, analysis of time-sequential imagery, and hydrodynamic modelling to investigate the histories of boulder groups identified by their intrinsic and contextual characteristics. Clear differences are revealed between the distribution of boulders recently moved and those of considerable age. Tracking the movement of boulders since 1957 confirms that storms of surprisingly frequent interval are capable of complex boulder movements, including lifting of megaclasts. Scrutiny of the ancient boulders, including weathering features and fascinating landward-facing (reverse) imbrication, cautiously suggests tsunami as the agent for their emplacement. A novel method is developed for depicting the velocity decay profiles of hypothetical waves, which overcomes some of the limitations of the Nott approach. Applied here, the wave run-up context further sets the ancient movers apart from their recent mover companions. The combined evidence implies a palimpsestic landscape where storm waves are regular geomorphic agents that add to and rework the distribution of boulders close to the shoreline, but over long time periods the landscape becomes reset by tsunami—a concept that is of value to agencies in Malta responsible for coastal safety, planning and management.
Sensitivity Analysis of the Rainfall–Runoff Modeling Parameters in Data-Scarce Urban Catchment
Authors: Ballinas-González Héctor A. , Alcocer-Yamanaka Víctor H. , Canto-Rios Javier J. , Simuta-Champo Roel
Published: October 2020

Rainfall–runoff phenomena are among the main processes within the hydrological cycle. In urban zones, the increases in imperviousness cause increased runoff, originating floods. It is fundamental to know the sensitivity of parameters in the modeling of an urban basin, which makes the calibration process more efficient by allowing one to focus only on the parameters for which the modeling results are sensitive. This research presents a formal sensitivity analysis of hydrological and hydraulic parameters—absolute–relative, relative–absolute, relative–relative sensitivity and R2—applied to an urban basin. The urban basin of Tuxtla Gutiérrez, Chiapas, in Mexico is an area prone to flooding caused by extreme precipitation events. The basin has little information in which the records (with the same time resolution) of precipitation and hydrometry match. The basin model representing an area of 355.07 km2 was characterized in the Stormwater Management Model (SWMM). The sensitivity analysis was performed for eight hydrological parameters and one hydraulic for two precipitation events and their impact on the depths of the Sabinal River. Based on the analysis, the parameters derived from the analysis that stand out as sensitive are the Manning coefficient of impervious surface and the minimum infiltration speed with R2 > 0.60. The results obtained demonstrate the importance of knowing the sensitivity of the parameters and their selection to perform an adequate calibration.
Assessment of the Impact of Climate Change on Snow Distribution and River Flows in a Snow-Dominated Mountainous Watershed in the Western Hindukush–Himalaya, Afghanistan
Authors: Azizi Abdul Haseeb , Asaoka Yoshihiro
Published: October 2020

Projected snow cover and river flows are important for planning and managing water resources in snow-dominated basins of the Himalayas. To quantify the impacts of climate change in the data scarce Panjshir River basin of Afghanistan, this study simulated present and future snow cover area (SCA) distributions with the snow model (SM), and river flows with the snowmelt runoff model (SRM). The SRM used the degree-day factor and precipitation gradient optimized by the SM to simulate river flows. Temperature and precipitation data from eight kinds of general circulation models (GCMs) were used for bias correction. The SM and SRM were first calibrated and validated using 2009–2015 data, and then bias-corrected future climate data were input to the models to simulate future SCA and river flows. Under both the representative concentration pathways (RCP) 4.5 and 8.5, the annual average SCA and river flow were projected to decrease in the mid and late 21st century, although seasonal increases were simulated in some instances. Uncertainty ranges in projected SCA and river flow under RCP 8.5 were small in the mid 21st century and large in the late 21st century. Therefore, climate change is projected to alter high-altitude stream sources in the Hindukush mountains and reduce the amount of water reaching downstream areas.
Simulating Urban Growth Scenarios Based on Ecological Security Pattern: A Case Study in Quanzhou, China
Authors: Liu Xiaoyang , Wei Ming , Zeng Jian
Published: October 2020

In recent decades, the ecological security pattern (ESP) has drawn increasing scientific attention against the backdrop of rapid urbanization and worsening ecological environment. Despite numerous achievements in identifying and constructing the ecological security pattern, limited attention has been paid on applying ESP to predict urban growth. To bridge the research gap, this paper took Quanzhou, China as a study case and incorporated the identified ESP into an urban growth simulation with three distinct scenarios. Following the “ecological source–ecological corridor–ecological security pattern” paradigm, the ESP identification was carried out from four single aspects (i.e., water, geology, biodiversity, and recreation) into three levels (i.e., basic ESP, intermediate ESP, and optimal ESP). Grounded in an equally weighted superposition algorithm, the four single ESPs were combined as an integrated ESP (IESP) with three levels. Taking IESP as an exclusion element, urban growth simulation in 2030 was completed with thee SLEUTH model. Drawing on the three levels of IESP, our urban growth simulation contained three scenarios. In terms of urban sprawl distribution coupled with urban growth rate, an optimal urban growth scenario is recommended in this paper to balance both urban development and eco-environment protection. We argue that our ESP-based urban growth simulation results shed new light on predicting urban sprawl and have the potential to inform planners and policymakers to contribute to more environmentally-friendly urban development.
Process Evaluation of a Participative Organizational Intervention as a Stress Preventive Intervention for Employees in Swedish Primary Health Care
Authors: Arapovic-Johansson Bozana , Jensen Irene , Wåhlin Charlotte , Björklund Christina , Kwak Lydia
Published: October 2020

This study is a process evaluation of a trial examining the effects of an organizational intervention (Productivity Measurement and Enhancement System or ProMES) on employee stress. The aims were to explore the implementation process and fidelity to the intervention guidelines, examine the influence of contextual factors (hindrances and facilitators) and explore participants’ experience of working with ProMES. We used the UK Medical Research Council (MRC) guidance to guide the process evaluation. The recruitment, reach and dose delivered were satisfactory and participation high. The employees felt ProMES clarified priorities, gave control and increased participation in decision-making. However, difficulty in obtaining statistical productivity data from the central administration office (a central feature of the intervention) hindered full implementation and regular feedback meetings. Staffing shortages interfered with the implementation process, while having seven design teams and one consultant prevented all occupational groups from working simultaneously. A detailed examination of access to necessary organizational data should be undertaken before implementing ProMES. We recommend a better introduction for new employees, more work on design and packaging and giving employees more training in how to use the software program. The study contributes to our understanding of process evaluations in research into organizational stress management interventions.
Effects of Chemotherapy Treatment on Muscle Strength, Quality of Life, Fatigue, and Anxiety in Women with Breast Cancer
Authors: Marques Vitor A. , Ferreira-Junior João B. , Lemos Thiago V. , Moraes Rafael F. , Junior José Roberto de S. , Alves Rafael R. , Silva Maria S. , Freitas-Junior Ruffo de , Vieira Carlos A.
Published: October 2020

The study aimed to evaluate the effects of chemotherapy treatment on muscle strength, quality of life, fatigue, and anxiety in women with breast cancer. Nineteen women who were undergoing a chemotherapy treatment (breast cancer treatment [BCT] group, 52.2 ± 13.1 years) and 18 women without cancer (control [CNT] group, 55.8 ± 8.4 years) answered questionnaires for evaluation of fatigue (Fatigue Scale), quality of life (Short-Form Healthy Survey [SF-36] questionnaire), and anxiety (State-Trait Anxiety Inventory [IDATE]) levels. Muscle strength was also assessed by an isometric grip test and an isokinetic knee extension test. Physical limitations, social and emotional domains of quality of life were lower in the BCT group in comparison to the CNT group (p = 0.002; p = 0.003; p = 0.0003, respectively). The other domains did not differ between groups (p > 0.05). There were no differences in fatigue and anxiety levels between both the BCT and CNT groups (p > 0.05). Additionally, isometric grip strength was higher in the CNT group when compared to the BCT group (p = 0.048). However, there were no differences between the BCT and CNT groups for peak torque and total work at both 60°.s−1 (p = 0.95 and p = 0.61, respectively) and 180°.s−1 (p = 0.94 and p = 0.72, respectively). These results suggest that three cycles of chemotherapy treatment may impair handgrip isometric strength and quality of life in women with breast cancer.
World Trade Center Health Program: First Decade of Research
Authors: Santiago-Colón Albeliz , Daniels Robert , Reissman Dori , Anderson Kristi , Calvert Geoffrey , Caplan Alexis , Carreón Tania , Katruska Alan , Kubale Travis , Liu Ruiling , Nembhard Rhonda , Robison W. Allen , Yiin James , Howard John
Published: October 2020

The terrorist attacks on 11 September 2001 placed nearly a half million people at increased risk of adverse health. Health effects research began shortly after and continues today, now mostly as a coordinated effort under the federally mandated World Trade Center (WTC) Health Program (WTCHP). Established in 2011, the WTCHP provides medical monitoring and treatment of covered health conditions for responders and survivors and maintains a research program aimed to improve the care and well-being of the affected population. By 2020, funds in excess of USD 127 M had been awarded for health effects research. This review describes research findings and provides an overview of the WTCHP and its future directions. The literature was systematically searched for relevant articles published from 11 September 2001 through 30 June 2020. Synthesis was limited to broad categories of mental health, cancer, respiratory disease, vulnerable populations, and emerging conditions. In total, 944 WTC articles were published, including peer-reviewed articles funded by the WTCHP (n = 291) and other sources. Research has focused on characterizing the burden and etiology of WTC-related health conditions. As the program moves forward, translational research that directly enhances the care of individuals with chronic mental and physical health conditions is needed.
Illicit Tobacco in Lithuania: A Cross-Sectional Survey
Authors: Liutkutė-Gumarov Vaida , Galkus Lukas , Petkevičienė Janina , Štelemėkas Mindaugas , Miščikienė Laura , Mickevičienė Aušra , Vaitkevičiūtė Justina
Published: October 2020

Taxation policies are the most cost-effective measure to reduce overall tobacco consumption. However, cigarettes in Lithuania are among the cheapest in the European Union. The threat of the illicit trade is often used to compromise evidence-based policies, pricing policies particularly. The aim of this study was to determine the extent of illicit cigarette consumption in Lithuania and identify the main characteristics of illicit cigarette smokers. The national cross-sectional survey with direct observation of the latest purchased pack of cigarettes was conducted between August and September 2019. In total, 1050 smokers aged ≥18 were interviewed face-to-face. The illicit share of the total consumption of cigarettes per year was 10.7% with 9.7% of smokers showing or describing illicit cigarette packs compared to 17% reported by industry-funded studies. Older smokers, smokers with lower education and heavy smokers were more likely to regularly purchase illicit cigarettes. The average price of an illicit pack was almost two times lower than licit. Although the illicit trade of tobacco products is a serious policy challenge, the threat of an increase in illicit trade should not delay tobacco taxation improvements.
Evaluation of Polish Wild Mushrooms as Beta-Glucan Sources
Authors: Mirończuk-Chodakowska Iwona , Witkowska Anna Maria
Published: October 2020

Mushroom beta-glucans show immunomodulatory, anticancer and antioxidant features. Numerous papers have been published in the last years on fungal polysaccharides, especially beta-glucans, demonstrating their various biological activities. However substantial data about beta-glucan contents in many mushroom species, especially wild mushrooms, are still missing. Therefore, the main objective of the study was to evaluate β-glucans in 18 species of wild mushrooms and three species of commercial mushrooms for comparison purposes. The contents of β-glucans were determined by the Megazyme method and with the Congo red method, which differ in analytical procedure. Among wild mushrooms, the highest mean β-glucan content assessed with the Megazyme method was found in Tricholoma portentosum (34.97 g/100 g DM), whereas with the Congo red method in Lactarius deliciosus (17.11 g/100 g DM) and Suillus grevillei (16.97 g/100 g DM). The β-glucans in wild mushrooms assessed with the Megazyme method were comparable to commercial mushrooms, whereas β-glucans assessed with the Congo red method were generally higher in wild mushrooms, especially in Russula vinosa, L. deliciosus and S. grevillei. This study indicates wild mushrooms as interesting material for β-glucan extraction for food industry and medicinal purposes.
Measuring Spatial Accessibility of Urban Fire Services Using Historical Fire Incidents in Nanjing, China
Authors: Mao Kainan , Chen Yuehong , Wu Guohao , Huang Junwang , Yang Wanying , Xia Zelong
Published: October 2020

The measurement of spatial accessibility of fire services is a key task in enhancing fire response efficiency and minimizing property losses and deaths. Recently, the two-step floating catchment area method and its modified versions have been widely applied. However, the circle catchment areas used in these methods are not suitable for measuring the accessibility of fire services because each fire station is often responsible for the fire incidents within its coverage. Meanwhile, most existing methods take the demographic data and their centroids of residential areas as the demands and locations, respectively, which makes it difficult to reflect the actual demands and locations of fire services. Thus, this paper proposes a fixed-coverage-based two-step floating catchment area (FC2SFCA) method that takes the fixed service coverage of fire stations as the catchment area and the locations and dispatched fire engines of historical fire incidents as the demand location and size, respectively, to measure the spatial accessibility of fire services. Using a case study area in Nanjing, China, the proposed FC2SFCA and enhanced two-step floating catchment area (E2SFCA) are employed to measure and compare the spatial accessibility of fire incidents and fire stations. The results show that (1) the spatial accessibility across Nanjing, China is unbalanced, with relatively high spatial accessibility in the areas around fire stations and the southwest and northeast at the city center area and relatively low spatial accessibility in the periphery and boundary of the service coverage areas and the core of the city center; (2) compared with E2SFCA, FC2SFCA is less influenced by other fire stations and provides greater actual fire service accessibility; (3) the spatial accessibility of fire services is more strongly affected by the number of fire incidents than firefighting capabilities, the area of service coverage, or the average number of crossroads (per kilometer). Suggestions are then made to improve the overall spatial access to fire services.
Lipid Emulsion Improves Functional Recovery in an Animal Model of Stroke
Authors: Tanioka Motomasa , Park Wyun Kon , Park Joohyun , Lee Jong Eun , Lee Bae Hwan
Published: October 2020

Stroke is a life-threatening condition that leads to the death of many people around the world. Reperfusion injury after ischemic stroke is a recurrent problem associated with various surgical procedures that involve the removal of blockages in the brain arteries. Lipid emulsion was recently shown to attenuate ischemic reperfusion injury in the heart and to protect the brain from excitotoxicity. However, investigations on the protective mechanisms of lipid emulsion against ischemia in the brain are still lacking. This study aimed to determine the neuroprotective effects of lipid emulsion in an in vivo rat model of ischemic reperfusion injury through middle cerebral artery occlusion (MCAO). Under sodium pentobarbital anesthesia, rats were subjected to MCAO surgery and were administered with lipid emulsion through intra-arterial injection during reperfusion. The experimental animals were assessed for neurological deficit wherein the brains were extracted at 24 h after reperfusion for triphenyltetrazolium chloride staining, immunoblotting and qPCR. Neuroprotection was found to be dosage-dependent and the rats treated with 20% lipid emulsion had significantly decreased infarction volumes and lower Bederson scores. Phosphorylation of Akt and glycogen synthase kinase 3-β (GSK3-β) were increased in the 20% lipid-emulsion treated group. The Wnt-associated signals showed a marked increase with a concomitant decrease in signals of inflammatory markers in the group treated with 20% lipid emulsion. The protective effects of lipid emulsion and survival-related expression of genes such as Akt, GSK-3β, Wnt1 and β-catenin were reversed by the intra-peritoneal administration of XAV939 through the inhibition of the Wnt/β-catenin signaling pathway. These results suggest that lipid emulsion has neuroprotective effects against ischemic reperfusion injury in the brain through the modulation of the Wnt signaling pathway and may provide potential insights for the development of therapeutic targets.
Assessing the Binding of Venoms from Aquatic Elapids to the Nicotinic Acetylcholine Receptor Orthosteric Site of Different Prey Models
Authors: Harris Richard J. , Youngman Nicholas J. , Zdenek Christina N. , Huynh Tam M. , Nouwens Amanda , Hodgson Wayne C. , Harrich David , Dunstan Nathan , Portes-Junior José A. , Fry Bryan G.
Published: October 2020

The evolution of an aquatic lifestyle from land dwelling venomous elapids is a radical ecological modification, bringing about many evolutionary changes from morphology to diet. Diet is an important ecological facet which can play a key role in regulating functional traits such as venom composition and prey-specific targeting of venom. In addition to predating upon novel prey (e.g., fish, fish eggs and invertebrates), the venoms of aquatic elapids also face the challenge of increased prey-escape potential in the aquatic environment. Thus, despite the independent radiation into an aquatic niche on four separate occasions, the venoms of aquatic elapids are evolving under convergent selection pressures. Utilising a biolayer interferometry binding assay, this study set out to elucidate whether crude venoms from representative aquatic elapids were target-specific to the orthosteric site of postsynaptic nicotinic acetylcholine receptor mimotopes of fish compared to other terrestrial prey types. Representatives of the four aquatic lineages were: aquatic coral snakes representative was Micrurus surinamensis;, sea kraits representative was Laticauda colubrina; sea snakes representatives were two Aipysurus spp. and eight Hydrophis spp; and water cobras representative was Naja annulata. No prey-specific differences in crude venom binding were observed from any species tested, except for Aipysurus laevis, which showed slight evidence of prey-potency differences. For Hydrophis caerulescens, H. peronii, H. schistosus and M. surinamensis, there was a lack of binding to the orthosteric site of any target lineage. Subsequent testing on the in vitro chick-biventer cervicis muscle preparation suggested that, while the venoms of these species bound postsynaptically, they bound to allosteric sites rather than orthosteric. Allosteric binding is potentially a weaker but faster-acting form of neurotoxicity and we hypothesise that the switch to allosteric binding is likely due to selection pressures related to prey-escape potential. This research has potentially opened up the possibility of a new functional class of toxins which have never been assessed previously while shedding light on the selection pressures shaping venom evolution.
Dendritic Cell and T Cell Crosstalk in Liver Fibrogenesis and Hepatocarcinogenesis: Implications for Prevention and Therapy of Liver Cancer
Authors: Lurje Isabella , Hammerich Linda , Tacke Frank
Published: October 2020

Liver fibrosis is a chronic, highly prevalent disease that may progress to cirrhosis and substantially increases the risk for development of hepatocellular carcinoma (HCC). Fibrotic livers are characterized by an inflammatory microenvironment that is composed of various immunologically active cells, including liver-resident populations (e.g., Kupffer cells, hepatic stellate cells and sinusoidal endothelium) and infiltrating leukocytes (e.g., monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). While inflammatory injury drives both fibrogenesis and carcinogenesis, the tolerogenic microenvironment of the liver conveys immunosuppressive effects that encourage tumor growth. An insufficient crosstalk between dendritic cells (DCs), the professional antigen presenting cells, and T cells, the efficient anti-tumor effector cells, is one of the main mechanisms of HCC tumor tolerance. The meticulous analysis of patient samples and mouse models of fibrosis-HCC provided in-depth insights into molecular mechanisms of immune interactions in liver cancer. The therapeutic modulation of this multifaceted immunological response, e.g., by inhibiting immune checkpoint molecules, in situ vaccination, oncolytic viruses or combinations thereof, is a rapidly evolving field that holds the potential to improve the outcome of patients with HCC. This review aims to highlight the current understanding of DC–T cell interactions in fibrogenesis and hepatocarcinogenesis and to illustrate the potentials and pitfalls of therapeutic clinical translation.
Apheresis in Neurological Disorders
Authors: Dorst Johannes
Published: October 2020

Plasma exchange (PE) and immunoadsorption (IA) constitute important options in the treatment of various autoimmune disorders across different medical disciplines [...]
PromarkerD Predicts Renal Function Decline in Type 2 Diabetes in the Canagliflozin Cardiovascular Assessment Study (CANVAS)
Authors: Peters Kirsten E. , Xu Jialin , Bringans Scott D. , Davis Wendy A. , Davis Timothy M.E. , Hansen Michael K. , Lipscombe Richard J.
Published: October 2020

The ability of current tests to predict chronic kidney disease (CKD) complicating diabetes is limited. This study investigated the prognostic utility of a novel blood test, PromarkerD, for predicting future renal function decline in individuals with type 2 diabetes from the CANagliflozin CardioVascular Assessment Study (CANVAS). PromarkerD scores were measured at baseline in 3568 CANVAS participants (n = 1195 placebo arm, n = 2373 canagliflozin arm) and used to predict incident CKD (estimated glomerular filtration rate (eGFR) <60 mL/min/1.73m2 during follow-up in those above this threshold at baseline) and eGFR decline ≥30% during the 4 years from randomization. Biomarker concentrations (apolipoprotein A-IV (apoA4), CD5 antigen-like (CD5L/AIM) and insulin-like growth factor-binding protein 3 (IGFBP3) measured by mass spectrometry were combined with clinical data (age, serum high-density lipoprotein (HDL)-cholesterol, eGFR) using a previously defined algorithm to provide PromarkerD scores categorized as low-, moderate- or high-risk. The participants (mean age 63 years, 33% females) had a median PromarkerD score of 2.9%, with 70.5% categorized as low-risk, 13.6% as moderate-risk and 15.9% as high-risk for developing incident CKD. After adjusting for treatment, baseline PromarkerD moderate-risk and high-risk scores were increasingly prognostic for incident CKD (odds ratio 5.29 and 13.52 versus low-risk, respectively; both p < 0.001). Analysis of the PromarkerD test system in CANVAS shows the test can predict clinically significant incident CKD in this multi-center clinical study but had limited utility for predicting eGFR decline ≥30%.
C. elegans Apical Extracellular Matrices Shape Epithelia
Authors: Cohen Jennifer D. , Sundaram Meera V.
Published: October 2020

Apical extracellular matrices (aECMs) coat exposed surfaces of epithelia to shape developing tissues and protect them from environmental insults. Despite their widespread importance for human health, aECMs are poorly understood compared to basal and stromal ECMs. The nematode Caenorhabditis elegans contains a variety of distinct aECMs, some of which share many of the same types of components (lipids, lipoproteins, collagens, zona pellucida domain proteins, chondroitin glycosaminoglycans and proteoglycans) with mammalian aECMs. These aECMs include the eggshell, a glycocalyx-like pre-cuticle, both collagenous and chitin-based cuticles, and other understudied aECMs of internal epithelia. C. elegans allows rapid genetic manipulations and live imaging of fluorescently-tagged aECM components, and is therefore providing new insights into aECM structure, trafficking, assembly, and functions in tissue shaping.
Absence of Azole Antifungal Resistance in Aspergillus fumigatus Isolated from Root Vegetables Harvested from UK Arable and Horticultural Soils
Authors: van der Torre Mireille H. , Whitby Cheryl , Eades Christopher P. , Moore Caroline B. , Novak-Frazer Lilyann , Richardson Malcolm D. , Rautemaa-Richardson Riina
Published: October 2020

The emergence of azole-resistant Aspergillus fumigatus (ARAf) complicates the treatment of aspergillosis and can nearly double the mortality from invasive aspergillosis (IA). ARAf has been isolated from many different environmental sites and indoor environments and thus presents a significant risk for susceptible patients. Local surveillance of environmental ARAf can guide antifungal prescribing and improve patient outcomes. In this study, seventy-four soils samples collected from the surface of a variety of root vegetables from farm shops and private gardens covering a wide geographical area of the UK, were cultured to assess the presence of A. fumigatus, and the prevalence and nature of any resistance mechanisms. A high-throughput in-house antifungal susceptibility screening method was developed and validated using the EUCAST MIC reference method, E.DEF 9.3.1. A total of 146 isolates were recovered and analysed. Even though the study premise was that soil-covered root vegetables and other fresh produce could represent a conduit for ARAf exposure in vulnerable patients, no ARAf were found in the soil samples despite 55% of samples harbouring A. fumigatus. The sample type and screening method used could be suitable for more extensive monitoring of the soil to detect trends in the prevalence of ARAf.
Inflammatory Responses in Oro-Maxillofacial Region Expanded Using Anisotropic Hydrogel Tissue Expander
Authors: Ali Salim Kholoud Mohamed , Abd Jalil Aminah , Radzi Zamri , Ismail Siti Mazlipah , Czernuszka Jan T. , Rahman Mohammad Tariqur
Published: October 2020

Objective: Reconstruction of oral and facial defects often necessitate replacement of missing soft tissue. The purpose of tissue expanders is to grow healthy supplementary tissue under a controlled force. This study investigates the inflammatory responses associated with the force generated from the use of anisotropic hydrogel tissue expanders. Methods: Sprague Dawley rats (n = 7, body weight = 300 g ± 50 g) were grouped randomly into two groups—control (n = 3) and expanded (n = 4). Anisotropic hydrogel tissue expanders were inserted into the frontal maxillofacial region of the rats in the expanded group. The rats were sacrificed, and skin samples were harvested, fixed in formalin, and embedded in paraffin wax for histological investigation. Hematoxylin and eosin staining was performed to detect histological changes between the two groups and to investigate the inflammatory response in the expanded samples. Three inflammatory markers, namely interleukin (IL)-1α, IL-6, and tumor necrosis factor-α (TNF-α), were analyzed by immunohistochemistry. Result: IL-1-α expression was only observed in the expanded tissue samples compared to the controls. In contrast, there was no significant difference in IL-6, and TNF-α production. Histological analysis showed the absence of inflammatory response in expanded tissues, and a negative non-significant correlation (Spearman’s correlation coefficient) between IL-1-α immune-positive cells and the inflammatory cells (r = −0.500). In conclusion, tissues that are expanded and stabilized using an anisotropic self-inflating hydrogel tissue expander might be useful for tissue replacement and engraftment as the expanded tissue does not show any sign of inflammatory responses. Detection of IL-1-α in the expanded tissues warrants further investigation for its involvement without any visible inflammatory response.
Effect of Thermal and Oxidative Aging on Asphalt Binders Rheology and Chemical Composition
Authors: Camargo Ingrid Gabrielle do Nascimento , Hofko Bernhard , Mirwald Johannes , Grothe Hinrich
Published: October 2020

Aging of asphalt binders is one of the main causes of its hardening, which negatively affects the cracking and fatigue resistance of asphalt binders. Understanding asphalt aging is crucial to improve the durability of asphalt pavements. In this regard, this study aims at understanding and differentiating the effect of temperature and oxygen uptake on the aging mechanisms of unmodified asphalt binders. For that, four laboratory aging procedures were employed. The two standardized procedures, rolling thin-film oven test (RTFOT) and pressure aging vessel (PAV), were considered to simulate the short-term and long-term aging of the asphalt binders, respectively. In addition, two thin-film aging test procedures, the nitrogen atmosphere oven aging test (NAAT) and ambient atmosphere oven aging test (OAAT) were employed to assess the effect of thermal and oxidative aging on unmodified asphalt binder properties. The NAAT procedure is based on the principle that the inert gas minimizes the oxidative aging. The rheological and chemical characterization showed that the high temperatures considered during the NAAT procedure did not change the properties of the unmodified asphalt binders. Therefore, it can be hypothesized that no significant thermal and oxidative aging was observed during NAAT aging procedure for the considered binders and that oxidative aging is the main cause for the hardening.