bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding intergroup relationships in public space through systematic observation / Comprensión de las relaciones intergrupales en el espacio público mediante la observación sistemática
Authors: Dionísio Tiago , Bernardo Fátima , Loupa-Ramos Isabel , Van den Driessche Jef , Van Eetvelde Veerle
Published: October 2024

This research focuses on exploring the intergroup relationship between migrants and natives through their use of public spaces. To comprehend these intergroup dynamics in a place, two case studies were analysed: an urban context in Belgium and Portugal. Through a systematic observation method, behavioural maps were collected to comprehend the distribution of natives and migrants in those areas, at different times of the day and their types of behaviours. Results showed that in the Belgian urban area, there is a sharing of the public space but with different behaviours, while in Portugal, we found a distribution of apparently migrant groups in space. In sum, the systematic observation of people in the public space provides a first assessment of the intergroup relations between migrants and natives in the urban context. It presents evidence to plan and design public space, promote social cohesion and comprehend what elements are relevant to maintain and adjust these groups’ place identity.
Surgical Specialty Outcome Differences for Major Spinal Procedures in Low-Acuity Patients
Authors: Price Anthony , File Christopher , LeBlanc Alvin , Fredricks Nathan , Ju Rylie , Pratt Nathan , Lall Rishi , Jupiter Daniel
Published: October 2024

Study Design

Retrospective Cohort Study.

Objectives

There is an ongoing debate as to the influence of specialty training on spine surgery. Alomari et al. indicated the influence of specialty on ACDF procedures. However, deeper analysis into other spine procedures and lower-acuity procedures has yet to occur. In this study, we aim to determine if the outcomes of the low American Society of Anesthesiologists (ASA) classification (ASA 1&2) patients undergoing spine surgery vary based on whether the operating surgeon was an orthopedic surgeon or a neurosurgeon.

Methods

The NSQIP databases from 2015 to 2021 were queried based on the CPT code for nine common spine procedures. Indicators of surgical course and successful outcomes were documented and compared between specialties.

Results

Neurosurgeons had minimally shorter operative times in the ASA 1&2 combined classification (ASA-C) group for cervical, lumbar, and combined spinal procedural groups. Neurosurgeons had a slightly lower percentage of perioperative transfusions in select ASA-C classes. Orthopedic surgeons had shorter lengths of stay for the cervical groups in ASA-C and ASA-1 classes (ASA-1). However, many specialty differences found in spine patients become less pronounced when considering only ASA-1 patients. Finally, postoperative complication outcomes and re-admission were similar between orthopedic and neurological surgeons in all cases.

Conclusions

These results, while statistically significant, are very likely clinically insignificant. They demonstrate that both orthopedic surgeons and neurosurgeons perform spinal surgery exceedingly safely with similarly low complication rates. This lays the groundwork for future exploration and benchmarking of performance in spine surgeries across neurosurgery and orthopedics.
Prisma Model-Driven Systematic Literature Review on the Effectiveness of Organisational Cultural Intelligence and Communication for Global Leadership Readiness
Authors: Resho  , Singh N. P.
Published: October 2024

Cultural intelligence and its associations with leadership (Earley & Ang, 2003, Cultural Intelligence: Individual Interactions Across Cultures, Stanford University Press, pp. 1–4) have been learned in developed countries. The occurrence, though, has not been widely learned in Indian organisations functioning in the worldwide setting. There is a shortage of analyses on cultural and communication challenges faced by Indian managers working in a multicultural setting. This reading examines the four major constructs: cultural intelligence, communication effectiveness, global leadership readiness and organisational effectiveness. The interface of the three constructs have also been discussed along with the frameworks. Studies published between 2010 and 2021 were identified from a systematic search of the PubMed, ResearchGate, ScienceDirect, Semantic Scholar, Scopus and Web of Science databases using the keywords ‘cultural intelligence’, ‘global leadership’, ‘business communication’ and ‘organisational effectiveness’. A total of 13 eligible studies were identified from 332 potentially relevant articles. The findings of the study were organised under four construct areas as mentioned in the objective. More research is needed in this area to gain knowledge so that global managers can work efficiently to achieve organisational effectiveness.
H-Loop Knotless Double-Row Repair Versus Krackow Repair for Achilles Tendon Rupture: A Biomechanical Study in a Cadaveric Model
Authors: Hou Jingyi , Li Yuxiang , Zheng Zhenze , Long Yi , Zhou Min , Yang Yitao , Yang Rui
Published: October 2024

Background:

Surgery is widely recognized as an effective treatment for Achilles tendon rupture; however, there remains debate regarding the optimal surgical approach.

Purpose:

To compare the biomechanical properties of 2 techniques, the H-loop knotless double-row (HLDR) suture repair and the Krackow suture repair, for Achilles tendon rupture in a cadaveric model.

Study Design:

Controlled laboratory study.

Methods:

Ten matched Achilles tendon specimens from 5 male and 5 female donors were obtained. Each specimen from a matched pair was randomly distributed to 1 of 2 repair groups, the HLDR group or the Krackow group. Tendon elongation was recorded after exposure to 10, 100, 200, 400, 600, 800, and 1000 load cycles. The gap distance after application of a 100-N force, the force needed to produce a 2-mm gap, and the load to failure were measured. All biomechanical properties were compared between the HLDR and Krackow groups using the paired t test.

Results:

The HLDR group consistently exhibited significantly less elongation than the Krackow group after exposure to the 7 load cycles ( P < .01 for all). In addition, the HLDR group exhibited a significantly smaller gap distance after applying a 100-N force (0.30 ± 0.02 vs 8.10 ± 0.46 mm for Krackow group), required significantly more force to generate a 2-mm gap (419.68 ± 39.48 vs 22.29 ± 3.40 N for the Krackow group), and had a significantly higher ultimate failure load (519.91 ± 57.29 vs 220.30 ± 19.27 N for the Krackow group) ( P < .01 for all).

Conclusion:

The study findings demonstrated that the HLDR technique had more advantages compared with the Krackow technique with regard to elongation after different cyclic loadings, gap distance after a 100-N load, force needed to produce a 2-mm gap, and load to failure in a cadaveric model.

Clinical Relevance:

The HLDR technique could be a viable option for Achilles tendon rupture repair.
Navigating US–China Rivalry in a Multi-aligned Middle East: Understanding India’s Shifting Strategy Towards the Region
Authors: Blarel Nicolas
Published: October 2024

Indian economic and strategic involvement in the Middle East has seen a transformation over the past two decades. This development has happened as many observers remark a gradual decline of US influence juxtaposed with China’s growing presence in the region. This article aims to assess how India has managed its bilateral relations with China within this region of increasing economic and strategic interest. Despite a growing literature on the China-India rivalry in Southeast Asia, South Asia or Central Asia, there is a notable dearth of scholarship on the China–India rivalry and its implications in the Middle East. Filling this gap, this article focuses on explaining how India has adapted its policies towards China’s rising influence in Middle Eastern politics. The core argument is that India’s strategy has been shaped by both a perception of declining US’ engagement in the Middle East and by opportunities created by new strategic hedging strategies from key regional players such as Israel, Saudi Arabia, the UAE and Iran. The article determines that India moved over the last decade from a strategy of buck-passing on US’ security provision to one of partial soft balancing of China’s strategic presence in the Middle East. However, the article also notes the limitations of standard theories of balance-of-power and strategic hedging to account for India’s multifaceted approach towards a regional order in flux.
Psychometric Validation of the Digital Competence Questionnaire for Nurses
Authors: Golz Christoph , Hahn Sabine , Zwakhalen Sandra M.G.
Published: October 2024

Introduction

No brief questionnaire comprising knowledge, skills, and attitudes is available to measure digital competence among clinical practice nurses.

Objective

The aim was to evaluate the structural validity and internal consistency of the Digital Competence Questionnaire (DCQ) for Clinical Practice Nurses.

Methods

A cross-sectional study was conducted with English-speaking clinical practice nurses. Twenty-six items from an initial item pool, developed in a prior conducted Delphi Study, were included. Exploratory factor analysis for structural validity with “oblimin” rotation and a two-factor solution as well as internal consistency test using Cronbach's alpha were conducted.

Results

Data from 185 nurses was obtained. The final questionnaire comprised of 12 items allocated to two factors: knowledge & skills and attitude. Factor “attitude” explained 33% of the variance and factor “knowledge & skills” 24%, resulting in a cumulative explanation of the variance of 57% by both factors. Internal consistency per factor was satisfactory, with 0.81 and 0.91, respectively.

Conclusion

The DCQ for clinical practice nurses is valid and has satisfactory internal consistency. Researchers and nurse managers can use it to assess the level of digital competence among clinical practice nurses. Future psychometric validation of the DCQ for clinical practice nurses is required to allow a conclusion on the goodness of fit and reliability.
Perceptions of Adult Obesity Education: A Pilot Study
Authors: Williams Seleda Ann , Sandholdt Cara Marie , Fine Jeffrey Robert , Mbe Kougang Anne
Published: October 2024

Objectives

This pilot research study, conducted at a large academic healthcare facility, used mixed methodology to (1) administer a survey to a group of primary care trainees and faculty and (2) conduct key informant interviews with the program directors, or their delegates of these primary care training programs, so as to gain insight into respondents’ perceptions about their training on adult obesity. To maintain confidentiality of the key informants, they were defined as “Administrators.” Faculty and trainees were from family medicine and internal medicine residency programs, as well as family nurse practitioner and physician assistant training programs.

Methods

This study used a quantitative survey and four qualitative key informant (Administrator) interviews. Descriptive statistics, χ2, or Fisher exact tests were used to analyze select survey responses. Administrator interviews were analyzed with thematic analysis.

Results

Survey respondents (n = 75) included primary care trainees (n=34), faculty (n=30), other (n=2), did not answer (n=9). Surveys indicated that additional training is needed for bariatric surgery, weight loss medications, and clinical nutrition. The three highest ranked topics in the surveys on adult obesity were basic nutrition, behavioral weight management, and a rotation on adult obesity. Most agreed on the need for interprofessional collaboration, a centralized obesity treatment center, and an introductory obesity course. Key themes from the four Administrator interviews revealed the need: for more training; to build upon current curriculum; use innovative technology; fiscal challenges; and time management.

Conclusions

Both faculty and trainees perceive that academic and clinical training on adult obesity is inadequate, and that trainees need more education on such topics as nutrition, physical activity, behavioral health, antiobesity medications, and bariatric surgery. Competency to treat varied by topic. It also showed that more interprofessional collaboration and a centralized obesity treatment center are needed. Recommendations included integrating modular units about obesity into already established primary care training programs and providing additional resources.
Association of Lesbian, Gay, Bisexual, and Transgender (LGBT) Cultural Competency Training With Provider Practice Characteristics and Perceptions of Patient Care
Authors: Hsiang Elaine , Ney John P. , Weathers Allison L. , Rosendale Nicole
Published: October 2024

BACKGROUND

While issues related to lesbian, gay, bisexual, and transgender (LGBT) health are increasingly incorporated into medical training, there remains limited guidance and opportunities for continuing medical education in LGBT health. It is unclear how participation in LGBT-specific training is distributed across physician specialties and practice regions. Additionally, national data assessing cultural competency training for physicians are scarce and do not delineate LGBT-specific training or training completed prior to, during, or after graduate medical education.

METHODS

Using data from the 2016 National Culturally and Linguistically Appropriate Services Physician Survey, this study evaluated patterns of post-residency cultural competency training, as well as associations between LGBT-specific training and provider perceptions of patient care outcomes.

RESULTS

Provider specialty, practice region, and receiving cultural competency training as a trainee were associated with post-residency LGBT-specific training. Surgical providers (odds ratio [OR]: 0.42; confidence interval [CI] 0.25–0.73; p = .002) and those practicing in the South (OR: 0.49; CI: 0.26–0.92; p = .025) had lower odds of completing LGBT-specific cultural competency training while in independent practice. Post-residency LGBT-specific training was associated with provider agreement that cultural competency training improves the quality of care (OR: 2.76; CI: 1.44–5.28; p = .002), patient satisfaction (OR: 2.55; CI: 1.32–4.93; p = .005), and patient comprehension (OR: 2.03; CI: 1.05–3.90; p = .034).

CONCLUSIONS

Our findings provide disaggregated analyses that nuance the assessment of cultural competency interventions and support a broader effort to increase attention to LGBT health in continuing medical education.
Efficacy assessment of intravenous immunoglobulin for gastrointestinal involvement in systemic sclerosis using UCLA SCTC GIT: Case-based review
Authors: Neto Marcelo , Albuquerque Fernando , Oliveira João , Cadório Maria João , Salvador Maria João , Santiago Tânia
Published: October 2024

Objectives:

To summarize the published evidence in the literature on the use of intravenous immunoglobulin in gastrointestinal tract involvement in systemic sclerosis patients and report the experience of our department.

Methods:

A systematic literature review was performed; and a literature search was conducted in MEDLINE and Embase until 1/5/2024, using the participants, intervention, comparator and outcomes framework. Only full-text articles involving systemic sclerosis adults, submitted to intravenous immunoglobulin (at least one administration) to treat primary gastrointestinal tract manifestations. The outcome was the University of California at Los Angeles Scleroderma Clinical Trial Consortium Gastrointestinal Tract 2.0 score to evaluate response to treatment. Two reviewers performed the assessment of data extraction and synthesis, independently.

Results:

Four papers (two case reports and two retrospective studies) out of 35 references were included. In addition, we added two systemic sclerosis patients from our department in this review. In 25 systemic sclerosis patients, with various gastrointestinal tract manifestations, the intravenous immunoglobulin therapy was found to improve digestive tract symptoms in SSc patients, as shown by the decrease in the scores of University of California at Los Angeles Scleroderma Clinical Trial Consortium Gastrointestinal Tract 2.0. No adverse events were reported, except for one case of low-grade fever post-administration.

Conclusion:

The results from this systematic literature review based on case series suggest that intravenous immunoglobulin may improve gastrointestinal tract symptoms assessed by the University of California at Los Angeles Scleroderma Clinical Trial Consortium Gastrointestinal Tract 2.0 scale, with only minor reported adverse events, suggestive of an acceptable safety profile. We believe that this systematic literature review will contribute to shed light on the efficacy and safety aspects of intravenous immunoglobulin treatment in the management of gastrointestinal tract symptoms; and multicenter randomized placebo-controlled trials are urgently needed to foster progress in this field.
An unusual case of thrombotic microangiopathy following autologous stem cell transplantation: A case report
Authors: Matarneh Ahmad , Sardar Sundus , Rivera Lidys , Abendroth Catherine , Karasinski Amanda , Abdulbasit Muhammad , Evans Mathew , Trivedi Naman , Verma Navin , Ghahramani Nasrollah
Published: October 2024

Thrombotic microangiopathy (TMA) is a rare but potentially life-threatening complication following autologous stem cell transplantation (ASCT). The incidence is extremely low. Here, we present the case of a 54-year-old male with relapsed diffuse large B-cell lymphoma (DLBCL) who developed TMA within 92 days after ASCT. The patient exhibited signs of hemolysis, thrombocytopenia and rapid renal function deterioration, and necessitating initiation of hemodialysis. Kidney biopsy confirmed TMA and Genetic testing revealed Complement H factor deficiency. Treatment with plasma exchange and corticosteroids resulted in no significant response. The patient was ultimately managed with ravulizumab. Transplant associated TMA (TA-TMA) is a an entity in which TMA occurs following stem cell transplant. This case highlights the importance of considering TMA in the differential diagnosis of renal failure following ASCT and initiating prompt treatment as delayed management can lead to devastating outcomes.
Digital twin for supporting decision-making and stakeholder collaboration in urban decarbonization processes. A participatory development in Gothenburg
Authors: Maiullari Daniela , Nageli Claudio , Rudena Andreas , Isacson Åsa , Dokter Giliam , Ellenbroek Ilse , Wallbaum Holger , Thuvander Liane
Published: October 2024

City Digital Twins have emerged as pivotal tools for representing and modelling urban systems. While existing literature emphasizes technological and framework development, limited attention has been given to the practical usability of digital twins in urban planning and decision-making processes. This paper addresses this gap by presenting a participatory approach for a city digital twin (CDTE) development, specifically tailored for supporting stakeholder communication and decision-making in the urban energy transition domain. The study, conducted in three phases, utilizes participatory methods, involving local stakeholders in the development process. The focus is on the Swedish city of Gothenburg, which is actively pursuing climate-neutral goals by 2030. The research integrates quantitative energy modelling and the creation of a web-based interface for the CDTE. The scenarios, grounded in local needs and challenges, explore the impacts of urban development models, climate warming and renovation measures on the building stock. The CDTE, developed and tested through workshops with diverse stakeholders, proves to be effective for the visualization and the discussion of various decarbonization scenarios. Key findings from users’ assessments underscore the significance of clarity and readability in scenario content and user interface for fostering interactions among different administrative spheres. This research contributes to the broader discourse on leveraging City Digital Twins for informed decision-making in urban contexts, providing insights into the practical application of digital twins in addressing the complex challenges of urban energy transition.
A process mining model to detect adverse patient safety events: Blood products watch
Authors: Sumer Ahmet Murat , Ceylan Cemil
Published: October 2024

Introduction

Patient safety incidents and adverse events are reported voluntarily by healthcare staff. The Global Trigger Tool, developed by the Institute for Healthcare Improvement, is used to capture unreported events. The objective of the new model is to use process mining in the context of adverse event detection to increase reporting efficiency.

Methods

In the first phase, the process mining model is used to discover the actual process map by querying inpatient processes using the provided log data. Transfusions of blood or blood products within 24 h after surgery, a subset of The Transfusion of Blood or Use of Blood Products trigger of Institute for Healthcare Improvement (GTT-C1) was deployed as a process pattern within the actual process map. Only detected patients were reviewed in the last phase.

Results

The model ran on a single hospital data set, which included 2870 patients, 1048 of which included surgeries. The model reduced the analyzed data to 57 patients. The expert group analyzed these 57 patients and defined 12 adverse events. The calculated adverse event ratio was 1.1%, comparable with other manual Global Trigger Tool studies. In addition, the reviewers’ time required decreased by 94.6%, from 138.28 to 7.52 h. Two reviewers validated the model by reviewing a sample of patients.

Conclusions

Process mining can be used in healthcare institutions to conduct routine surveillance and detect unreported safety events. This allows healthcare providers to allocate financial and human resources to more urgent needs and increase efficiency. Further research is needed to evaluate the model with other triggers.
Virtual Reality Surgical Planning of a Complex Two-Jaw Defect
Authors: Bou Zeid Naji , Moradian Simon , Micallef Christopher J. , Purnell Chad A. , Patel Pravin K. , Schierle Clark , Alkureishi Lee
Published: October 2024

Head and neck reconstruction poses challenges requiring stable coverage and optimal appearance and functionality. In complex cases, and beyond the right choice of flap, Virtual Reality (VR) surgical software and preoperative planning, are needed to optimize outcomes. Our patient is a 16-year-old with a history of rhabdomyosarcoma necessitating maxillary-mandibular defect reconstruction. VR-based preoperative planning facilitated the intervention by creating 3D-VR models of facial anatomy and fibula, simulating resection margins, and creating surgical guides for intraoperative use. Our 2-team surgeons opted for a single-staged reconstruction using a vascularized fibula free flap. This case demonstrates successful single-flap reconstruction of a maxillary-mandibular defect, addressing challenges of recipient vessel scarcity, fibrotic scarring, and poor tissue quality. It also underscores the efficacy of a 2-team approach and VR-based preoperative planning in achieving successful outcomes in complex maxillary-mandibular reconstruction. The use of a single fibula flap minimized possible complications and operative time, and the 2-team approach helped by combining the expertise and training of craniofacial and microsurgical trained surgeons. Acknowledging limitations in imaging quality and real-time adaptability, we advocate for continued exploration of VR technologies in enhancing surgical precision in challenging cases.
Life cycle assessment of a virtual power plant: Evaluating the environmental performance of a system utilising solar photovoltaic generation and batteries
Authors: Pimentel Pincelli Isabella , Hinkley Jim , Brent Alan
Published: October 2024

As the world shifts to renewable energy sources to mitigate climate change, virtual power plants (VPPs) have emerged as an innovative solution for integrating distributed renewable generation. This study conducted a comprehensive Life Cycle Assessment (LCA) for a 40 MW VPP in operation in Aotearoa New Zealand, comprising residential solar photovoltaic systems with battery storage. Unlike traditional LCA studies that focus on individual components, this study evaluates the full life cycle impacts of a VPP, offering a holistic view of its environmental implications. Additionally, the study considered electricity fed back to the grid, which avoids grid electricity, or electricity generation from natural gas. The findings reveal an estimated life cycle greenhouse gas (GHG) emissions of 45.3–78.9 gCO2eq/kWh for the VPP, depending on what the surplus electricity replaces. Notably, avoiding natural gas electricity generation by returning surplus electricity to the grid yields a significant credit of −47.3 gCO2eq/kWh. The life cycle GHG emissions of the VPP are highly sensitive to PV generation. If the systems are operated at their maximum potential, the overall emissions reduce by 17.6%. Conversely, when operated at minimum potential, the emissions increase by 23.2%. Additionally, uncertainties in the energy demand to manufacture the battery cells can alter GHG emissions from a 6.7% reduction to a 14.3% increase. This study underscores the complexity of evaluating environmental performances of VPPs and fills a gap in the literature by presenting the potential environmental impacts and benefits of VPPs, shedding light on their role in fostering sustainability with the energy transition.
Large-scale education in respiratory medicine: contentversusdelivery
Authors: Kelly Emer , Costello Richard W.
Published: October 2024

The respiratory literature, both written and in online formats, is growing exponentially. Capturing quality content, to meet the learning needs of those working in all fields of respiratory medicine and delivering it in a palatable, accessible format is challenging but paramount. In this article we discuss ways to determine the information content and review different methods of delivering this content to those who need it.
Emergency Knowledge Translation, COVID-19 and indoor air: evaluating a virtual ventilation and filtration consultation program for community spaces in Ontario
Authors: Katz Amy , Li Tianyuan , James LLana , Buhariwala Pearl , Osei-Twum Jo-Ann , Siegel Jeffrey , O’Campo Patricia
Published: October 2024

Background

An October, 2021 review of Public Health Ontario's COVID-19 guidance for congregate settings such as shelters and long-term care homes demonstrated that this guidance did not include references to ventilation or filtration. In April 2022, an interdisciplinary team with expertise in indoor air quality (IAQ), engineering, epidemiology, community programming and knowledge translation launched a virtual ventilation and filtration consultation program for community spaces in Toronto, Ontario. The program gives people working in community spaces direct access to IAQ experts through 25-min online appointments. The program aims to help reduce the risk of COVID-19 transmission in community spaces, and was designed to help compensate for gaps in public health guidance and action.

Methods

Representatives from participating organizations (n. 27) received a link to an online survey via email in April 2023. Survey questions explored the impacts of the program on topics such as: purchase and use of portable air filters; maintenance and use of bathroom fans; and, maintenance and modification of HVAC systems. Survey participation was anonymous, and no demographic information was collected from participants.

Results

Representatives from 11 organizations completed the survey (40%). Of those who responded, nine (82%) made changes as a result of the program, with eight (73%) making two or more changes such as purchasing portable air filters and increasing routine maintenance of HVAC systems.

Conclusions

When presented with brief access to expert support and tailored plain language guidance, people working in community spaces increased their use of ventilation and filtration strategies for COVID-19 infection prevention and control.
Of the article: evaluation of the masticatory efficiency of children with stainless steel crowns: a pilot cross-sectional split-mouth study
Authors: Sen Madhura , Yarmunja Mahabala Karuna , Natarajan Srikant , Gupta Deveshi , Shekhar Shrehya , Rao Ashwin , Nayak P Anupama
Published: October 2024

Background

Stainless steel crowns (SSCs) are commonly employed to restore the posterior teeth of children and over the years, they are available with improved anatomical shapes. This study was conducted to evaluate and assess the effect of the placement of SSCs on the masticatory efficiency of children.

Methods

This pilot cross-sectional split-mouth study assessed masticatory efficiency in children aged 6–11 years. Fifteen participants, each with stainless-steel crowns placed unilaterally at least one month prior, were included. This study utilized two flavours of trident chewing gum (red and green) to measure masticatory efficiency. The child was asked to chew half a strip of red and green chewing gum placed one on top of the other using either the noncrown or crown side 15 times. Another set of chewing gum was given to the child to chew 20 times on the same side. The chewing exercise was repeated using teeth on the other side. Chewed gum samples were collected, photographed, and analysed via ImageJ software to determine the red and green areas. Masticatory efficiency was subsequently calculated with the help of a formula in which the red areas and green areas were calculated via ImageJ software. The data were analysed with paired t tests via SPSS (version 20.0).

Results

When the mean values of the crown side were compared with those of the noncrown side following 15 chewing cycles, the chewing efficiency on the noncrown side was greater, with a difference of 0.303, whereas the chewing efficiency following 20 chewing cycles was greater on the crown side, with a difference of 0.814. However, both differences were statistically nonsignificant, with t values of -0.07 and 0.26, respectively, and p values of 0.94 and 0.8, respectively.

Conclusion

The presence of SSCs on the molars of children did not affect masticatory efficiency.
Recommended recumbency to avoid insertional complications during small-bore wire-guided thoracostomy tube placement in cats—a cadaver study
Authors: Siegelmayer Desiree , Schnabl-Feichter Eva , Tichy Alexander , Gradner Gabriele , Degasperi Brigitte , Liehmann Lea
Published: October 2024

Background

Small-bore wire-guided thoracostomy tubes (SBWGTT) are commonly used in cats to manage pleural disease and generally have a low complication rate. Our study aimed to explore the correlation between recumbency of cats, placement method, and the occurrence of insertional complications to identify risk factors during SBWGTT placement.

In this experimental cadaveric study, SBWGTT placement using a modified Seldinger technique was conducted in 24 feline cadavers. Cats, euthanized for reasons unrelated to the study, were randomly assigned to pleural effusion (EFF; n = 12) and pneumothorax (PNEU; n = 12) groups. Each cadaver was intubated and ventilated with a peak inspiratory pressure (PIP) of 10 mmHg, and sterile saline or air was instilled into the thorax over a 5 mm thoracoscopic trocar in the fourth intercostal space (ICS). Instillation was stopped when the lateral thoracic wall to lung distance (TWLD) reached 10 to 12 mm, measured with ultrasound in the favorable position. Sternal recumbency was the favorable position for the EFF group, and lateral recumbency for the PNEU group. Following the placement of the first SBWGTT in each group, the cadavers were positioned unfavorably (lateral recumbency for EFF group, sternal recumbency for PNEU group), and a second drain was introduced contralaterally. A bilateral 8th ICS thoracotomy was then performed to visually assess intrathoracic structures and drain integrity.

A binary logistic regression mixed model was conducted to determine interaction between the induced condition and body position.

Results

A total of 48 SBWGTTs were placed, with complications observed in 33.3% (8/24) of cases. Five of these were major complications consisting of lung lacerations. Complications were more common in the unfavorable position, accounting for 75% of cases, although this result was not statistically significant. The odds of complication rates were > 70% in the unfavorable position and decreased with an increase in TWLD (< 30%).

Conclusion

Complications associated with SBWGTT placement are influenced by recumbency, although the data did not reach statistical significance. Placing cats in lateral recumbency for pneumothorax treatment and sternal recumbency for pleural effusion treatment may reduce insertional complications.
A single-center, double-blind, randomized, placebo-controlled, two-arm study to evaluate the safety and efficacy of once-weekly sirolimus (rapamycin) on muscle strength and endurance in older adults following a 13-week exercise program
Authors: Stanfield Brad , Kaeberlein Matt , Leroux Brian , Jones Julie , Lucas Ruth , Arroll Bruce
Published: October 2024

Background

Aging leads to a decline in muscle mass and strength, contributing to frailty and decreased quality of life. Sirolimus (rapamycin) , an mTOR inhibitor, has shown potential in preclinical studies to extend lifespan and improve health span. This study evaluates the safety and efficacy of once-weekly sirolimus (rapamycin) administration on muscle strength and endurance in older adults engaged in a 13-week exercise program.

Methods

This randomized, double-blind, placebo-controlled trial will enroll 40 participants aged 65–85. Participants will be randomly assigned to receive either sirolimus (rapamycin) 6 mg/week or placebo for 13 weeks, in conjunction with an at-home exercise program. The primary outcome measure is the change in muscle strength and endurance, assessed by the 30-Second Chair-Stand Test. Secondary outcome measures include adverse events, changes in muscle strength and endurance as measured by the 6-min walk test, handgrip strength, and participant-reported outcomes using the SF-36 survey. Assessments will be conducted at baseline, mid-intervention (week 6), and post-intervention (week 13). Blood samples will be collected for hematology and biochemistry analyses, including full blood count, urea and electrolytes, liver function tests, HbA1c, lipids, serum IGF-1, and hs-CRP. DNA methylation will be analyzed using TruDiagnostic™ to explore changes in biological age.

Discussion

This study aims to provide insights into the potential benefits of intermittent sirolimus (rapamycin) administration on muscle performance in older adults. By alternating periods of mTOR inhibition through rapamycin and activation via exercise, this study will explore a novel approach to enhancing muscle strength and endurance in the aging population. The results could have significant implications for developing interventions to improve physical function and overall health outcomes in older adults. Safety and tolerability will also be closely monitored to ensure the feasibility of this regimen for wider application.

Trial registration

Australia New Zealand Clinical Trials Registry, ACTRN12624000790549. Registered on 26 June 2024 https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12624000790549.
Contamination of trichlorobenzene isomers in food: toxicity, analytical methods, occurrence in food, and risk assessments
Authors: Lee Hyegyeong , Kim Kiyun , Park Junhyeong , Lee Joon-Goo
Published: October 2024

Trichlorobenzenes (TCBs), comprising the isomers 1,2,3-, 1,2,4-, and 1,3,5-TCB, disrupt metabolic processes by inducing liver enzymes involved in xenobiotic metabolism, suggesting a broad toxicological impact. Specifically, exposure to TCBs is associated with significant organ-specific toxicities, such as increased liver and kidney weights in rodents and cytotoxic effects in mammalian cells, which include DNA damage without metabolic activation. Used extensively in industrial and agricultural sectors, TCBs are prevalent pollutants in various ecosystems, including air, food, surface water, groundwater, sediment, soil, and sewage. This is a concern because of their tendency to accumulate in lipid-containing tissues of animals and humans and potentially serious risks to human health and ecosystems. Information showing the presence of TCBs in food, drinking water, and even human breast milk underscores the need for ongoing assessment of the extent of these contaminants in food to measure the potential exposure to these chemicals. TCBs are extracted from various food sample matrices, and then instrumental analysis is performed, typically gas chromatography (GC) coupled with a variety of detectors. This review discusses the occurrence and risk assessment of TCBs in foods, as well as the toxicology and analytical methods related to TCBs.
Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation
Authors: Jia Lei , Zhang Wenying , Luo Tao , Li Yongtao , Shu Jianhong , Strand Julie , Yue Yuan , Purup Stig , Liu Jianxin , Shi Hengbo
Published: October 2024

Background

Although several cell culture systems have been developed to investigate the function of the mammary gland in dairy livestock, they have potential limitations, such as the loss of alveolar structure or genetic and phenotypic differences from their native counterparts. Overcoming these challenges is crucial for lactation research. Development of protocols to establish lactating organoid of livestock represents a promising goal for the future. In this study, we developed a protocol to establish a culture system for mammary organoids in dairy goats to model the mammary gland development and lactation process.

Results

The organoids cultured within an extracellular matrix gel maintained a bilayer structure that closely resembled the native architecture of mammary tissue. The expansion of mammary organoids was significantly promoted by growth factors containing epidermal growth factor and fibroblast growth factor 2 whereas the proliferative index of the organoids was significantly inhibited by the treatment with WNT inhibitors. Upon stimulation with a lactogenic medium containing prolactin, the mammary organoids exhibited efficient lactation, characterized by the accumulation of lipid droplets in the lumen space. The lactation could be sustained for more than 3 weeks. Importantly, the expression patterns of genes related to fatty acid synthesis and milk proteins in lactating organoids closely mirrored those observed in mammary tissues. These observations were confirmed by data from proteomic analysis that the bulk of milk proteins was produced in the lactating organoids.

Conclusion

This study is the first to establish a mammary organoid culture system modeling the mammary gland development and lactation process in ruminants. The efficient induction of lactation in ruminant mammary organoids holds promises for advancing the field of cell-based milk bio-manufacture in the food industry.
Combined inhibition of MET and VEGF enhances therapeutic efficacy of EGFR TKIs in EGFR-mutant non-small cell lung cancer with concomitant aberrant MET activation
Authors: Huang Shanshan , Long Yaling , Gao Yuan , Lin Wanling , Wang Lei , Jiang Jizong , Yuan Xun , Chen Yuan , Zhang Peng , Chu Qian
Published: October 2024

Background

Aberrant activation of mesenchymal epithelial transition (MET) has been considered to mediate primary and acquired resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) in EGFR-mutant non-small cell lung cancer (NSCLC). However, mechanisms underlying this process are not wholly clear and the effective therapeutic strategy remains to be determined.

Methods

The gefitinib-resistant NSCLC cell lines were induced by concentration increase method in vitro. Western blot and qPCR were used to investigate the relationship between MET and vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) signaling pathway. Double luciferase reporter gene and co-immunoprecipitation were used to further reveal the regulation mechanism between MET and VEGF/VEGFR2. The effect of combined inhibition of MET and VEGF/VEGFR2 signaling pathway on the therapeutic sensitivity of EGFR-TKI in gefitinib resistant cell lines with MET aberration was verified ex vivo and in vivo.

Results

We successfully obtained two gefitinib-resistant NSCLC cell lines with EGFR mutation and abnormal activation of MET. We observed that MET formed a positive feedback loop with the VEGF/VEGFR2 signaling, leading to persistent downstream signaling activation. Specifically, MET up-regulated VEGFR2 expression in a MAPK/ERK/ETS1-dependent manner, while VEGF promoted physical interaction between VEGFR2 and MET, thereby facilitating MET phosphorylation. A MET inhibitor, crizotinib, combined with an anti-VEGF antibody, bevacizumab, enhanced the sensitivity of NSCLC cells to gefitinib and synergistically inhibited the activation of downstream signaling in vitro. Dual inhibition of MET and VEGF combined with EGFR TKIs markedly restrained tumor growth in both human NSCLC xenograft models and in an EGFR/MET co-altered case.

Conclusions

Our work reveals a positive feedback loop between MET and VEGF/VEGFR2, resulting in continuous downstream signal activation. Combined inhibition of MET and VEGF/VEGFR2 signaling pathway may be beneficial for reversing EGFR TKIs resistance.
The relationship between co-occurring traumatic experiences and co-occurring mental health domains for veterans resident in Northern Ireland
Authors: Hitch Catherine , Spikol Erik , Toner Paul , Armour Cherie
Published: October 2024

Introduction

Veterans residing in Northern Ireland (NI) likely experience higher levels of co-occurring lifetime trauma exposure and associated co-occurring mental health symptoms, due to the Troubles. As NI veterans have been subject to little exploration it is difficult to know how to support them. This exploratory study explored the co-occurrence of mental health symptoms as a function of co-occurring traumatic experiences.

Methods

Two latent class analyses (LCA) were conducted on NI veteran data (n = 609). One LCA factored endorsements of 16-lifetime traumatic events, with a separate LCA exploring the co-occurrence of symptoms across six mental health domains. Mental health symptom classes were considered as a function of trauma classes, military-specific variables, gender and age.

Results

Three trauma classes were identified: High Multi Trauma (10.84%); High Combat/Conflict (47.62%); Moderate Combat Conflict (41.54%), and three mental health symptom classes: High Co-occurring Mental Health (19.38%); High Depression Moderate Anxiety/Alcohol (24.63%); Moderate Alcohol/Normative (55.99%). Significant predictors of High Co-occurring Mental Health class membership were UDR service, ‘Worst’ military trauma and High Multi Trauma class membership. Both combat classes had a negative relationship with the High Co-occurring Mental Health. Predictors of the High Depression Moderate Anxiety/Alcohol class were High Multi Trauma class membership and UDR service, with Age and Moderate Combat Class membership having a negative relationship.

Discussion

NI veterans could be labelled as ‘traumatised’ due to high levels of combat/conflict exposure, yet the two combat classes seemed unrelated to membership of poorer mental health classes. UDR membership indicated that living in one’s theatre of war could have mental health implications. It was concerning that 45% experienced some co-occurring mental health symptoms with 19% experiencing the poorest symptoms. Hazardous alcohol use appeared unrelated to poor mental health. Further research is needed, utilising robust methods. No clinical inferences are to be made from this exploratory study.
An early and stable mouse model of polymyxin-induced acute kidney injury
Authors: Liu Linqiong , Liu Yuxi , Xin Yu , Liu Yanqi , Gao Yan , Yu Kaijiang , Wang Changsong
Published: October 2024

Background

Polymyxins have been revived as a last-line therapeutic option for multi-drug resistant bacteria and continue to account for a significant proportion of global antibiotic usage. However, kidney injury is often a treatment limiting event with kidney failure rates ranging from 5 to 13%. The mechanisms underlying polymyxin-induced nephrotoxicity are currently unclear. Researches of polymyxin-associated acute kidney injury (AKI) models need to be more standardized, which is crucial for obtaining consistent and robust mechanistic results.

Methods

In this study, male C57BL/6 mice received different doses of polymyxin B (PB) and polymyxin E (PE, also known as colistin) by different routes once daily (QD), twice daily (BID), and thrice daily (TID) for 3 days. We continuously monitored the glomerular filtration rate (GFR) and the AKI biomarkers, including serum creatinine (Scr), blood urea nitrogen (BUN), neutrophil gelatinase-associated lipocalin (NGAL), and kidney injury molecule-1 (KIM-1). We also performed histopathological examinations to assess the extent of kidney injury.

Results

Mice receiving PB (35 mg/kg/day subcutaneously) once daily exhibited a significant decrease in GFR and a notable increase in KIM-1 two hours after the first dose. Changes in GFR and KIM-1 at 24, 48 and 72 h were consistent and demonstrated the occurrence of kidney injury. Histopathological assessments showed a positive correlation between the severity of kidney injury and the changes in GFR and KIM-1 (Spearman’s rho = 0.3167, P = 0.0264). The other groups of mice injected with PB and PE did not show significant changes in GFR and AKI biomarkers compared to the control group.

Conclusion

The group receiving PB (35 mg/kg/day subcutaneously) once daily consistently developed AKI at 2 h after the first dose. Establishing an early and stable AKI model facilitates researches into the mechanisms of early-stage kidney injury. In addition, our results indicated that PE had less toxicity than PB and mice receiving the same dose of PB in the QD group exhibited more severe kidney injury than the BID and TID groups.