bluuebunny/crossref_metadata_2025 huggingface dataset:
Genotype-specific human papillomavirus detection in cervical smears.
Authors: Szostek Slawa , Klimek Malgorzata , Zawilinska Barbara , Kosz-Vnenchak Magdalena
Published: November 2019

Human papillomavirus (HPV) is widely accepted as a causative agent of cervical cancer. The distribution and prevalence of HPV types depend on geographic region and demographic factors. The aim of this study was to investigate the relationship between the presence of various HPV types and the outcome of cytological examination. Cervical smears were obtained from 125 women from southern Poland: low grade squamous intraepithelial lesions (LSIL) - 44, high grade squamous intraepithelial lesions (HSIL) - 12, cervical carcinoma - 27 and 42 women without abnormality in cytology as a control group. DNA was extracted from the smears and broad-spectrum HPV DNA amplification and genotyping was performed with the SPF 10 primer set and reverse hybridisation line probe assay (INNO-LiPA HPV Genotyping, Innogenetics). HPV DNA was detected in approximately 72% cases, more frequently in women with squamous intraepithelial lesions and cervical carcinoma than in the control group (P < 0.0005). The most frequent type found was HPV 16 (37%), followed by HPV 51 (28%) and HPV 52 (17%). A single HPV type was detected in 51% positive cases, more frequently in cervical cancer specimens. Multiple HPV infection was dominant in women with LSIL and normal cytology. Prevalence of HPV 16 increased with the severity of cervical smear abnormality. For women HPV 16 positive, the relative risk (odds ratio) of the occurrence of HSIL and cervical cancer versus LSIL was 14.4 (95% CI, 3.0-69.2; P=0.001) and 49.4 (95% CI, 6.5-372.8; P < 0.001), respectively. Genotyping of HPV will allow better classification of women with cervical abnormalities into different risk groups and could be useful in therapy.
Detection of specific lytic and latent transcripts can help to predict the status of Epstein-Barr virus infection in transplant recipients with high virus load.
Authors: Zawilinska Barbara , Kosinska Anna , Lenart Marzena , Kopec Jolanta , Piatkowska-Jakubas Beata , Skotnicki Aleksander , Kosz-Vnenchak Magdalena
Published: November 2019

Epstein-Barr virus (EBV), a member of the family Herpesviridae, is widely spread in the human population and has the ability to establish lifelong latent infection. In immunocompetent individuals the virus reactivation is usually harmless and unnoticeable. In immunocompromised patients productive infection or type III latency may lead to EBV-associated post-transplant lymphoproliferative disorder (PTLD). The aim of our research was to investigate the utility of PCR-based methods in the diagnosis and monitoring of EBV infections in bone marrow transplant recipients. Thirty-eight peripheral blood leukocyte samples obtained from 16 patients were analysed, in which EBV DNA was confirmed by PCR. We used semi-quantitative PCR to estimate the viral load and reverse-transcription PCR (RT-PCR) to differentiate between latent and productive EBV infection. In 14 patients we confirmed productive viral infection. We observed a correlation between higher number of EBV genome copies and the presence of transcripts specific for type III latency as well as clinical symptoms.
Specific binding sites for progesterone and 17beta-estradiol in cells of Triticum aestivum L.
Authors: Janeczko Anna , Budziszewska Bogusława , Skoczowski Andrzej , Dybała Małgorzata
Published: November 2019

The presence and location of specific binding sites for progesterone and 17beta-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [(3)H]progesterone and [(3)H]17beta-estradiol occurs in wheat cells. The binding sites are located in membranes and in the cytosol. Specific binding of [(3)H]17beta-estradiol is higher in the membranes than in the cytosol. Specific binding of both ligands in the cytosolic fraction is higher in vernalized plants than in non-vernalized ones. The possibility of the occurrence of steroid binding proteins specific for progesterone and 17beta-estradiol, putative steroid receptors for these steroids in Triticum aestivum L., is discussed.
Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes.
Authors: Piao Xianyu , Sun Liancheng , Zhang Tianbiao , Gan Youlin , Guan Yifu
Published: November 2019

Effective discrimination of non-complementary nucleotides is an important factor to ensure the accuracy of hybridization-based nucleic acid analyses. The current study investigates the effects of the chemical nature, the positions, the numbers, and the cooperative behavior of mismatches as well as insertions on 20-mer and 30-mer duplexes. We observed the hybridization stability trend affected by mismatches: G:T approximately G:G > G:A > A:A approximately T:T > A:C approximately T:C > C:C. The experimental data show that mismatches at the center of the oligonucleotide probes have a more profound destabilizing effect on the hybridization stability than those at either ends. Insertions also demonstrate a similar destabilizing effect as mismatches. These results provide useful information for designing DNA microarray nucleotide probes and for improving the discrimination accuracy of hybridization-based detections.
Heterologous expression and initial characterization of recombinant RbcX protein from Thermosynechococcus elongatus BP-1 and the role of RbcX in RuBisCO assembly.
Authors: Tarnawski Miroslaw , Gubernator Beata , Kolesinski Piotr , Szczepaniak Andrzej
Published: November 2019

In the cyanobacterial RuBisCO operon from Thermosynechococcus elongatus the rbcX gene is juxtaposed and cotranscribed with the rbcL and rbcS genes which encode large and small RuBisCO subunits, respectively. It has been suggested that the rbcX position is not random and that the RbcX protein could be a chaperone for RuBisCO. In this study, the RbcX protein from T. elongatus was overexpressed, purified and preliminary functional studies were conducted. The recombinant protein purified from Escherichia coli extracts was predominantly present in a soluble fraction in a dimeric form. Coexpression experiments have demonstrated that RbcX can mediate RbcL dimer (L(2)) formation, and that it is essential for the L(8) core complex assembly. This is the first characterization of the RbcX protein from a thermophilic organism.
Conserved Cys residue influences catalytic properties of potato endo-(1-->3)-beta-glucanase GLUB20-2.
Authors: Witek Agnieszka I , Witek Kamil , Hennig Jacek
Published: November 2019

The synthesis and degradation of (1-->3)-beta-glycosidic bonds between glucose moieties are essential metabolic processes in plant cell architecture and function. We have found that a unique, conserved cysteine residue, positioned outside the catalytic centre of potato endo-(1-->3)-beta-glucanase - product of the gluB20-2 gene, participates in determining the substrate specificity of the enzyme. The same residue is largely responsible for endo-(1-->3)-beta-glucanase inhibition by mercury ions. Our results confirm that the spatial adjustment between an enzyme and its substrate is one of the essential factors contributing to the specificity and accuracy of enzymatic reactions.
Does Diabetic Microvascular Complications Affect Gastrointestinal Symptoms?
Authors: Ghadiri-Anari Akram , Gholami Somayeh , Sheyda Elham , Kharazmi Shadab , Namiranian Nasim
Published: November 2019

Abstract- Due to high prevalence of diabetes in our region (16.3%) and no data on the frequency of gastrointestinal (GI) symptoms in this population, we performed a cross-sectional study to evaluate the frequency of GIsymptoms in diabetic patients and its association between microvascular complications (retinopathy and nephropathy) and gastrointestinal symptoms in diabetic subjects.This analytical crosssectional study was conducted from 2014 to 2016 on 233 patients with type 2 diabetes mellitus (T2DM), 3065-year, referred to Yazd diabetic research center. They were selected by convenient sample method. A questionnaire according to Rome III Criteria was used to collect digestive information related to diabetes. Last HbA1c (Since 2-3 months ago) was available in the patient's medical folder. Diabetic nephropathy defines to increased excretion rate of albumin in the urine in the range of above 30 mg/g creatinine. Diabetic retinopathy was examined by an expert ophthalmologist (retinal specialist). For the current study, 233 patients (age 30-65 years with mean age of 57.43±10.49 years, 102 (43.8%) males and 131 (56.2%) females) were included. Among 233 patients, 91 cases (39.1%) had nephropathy,and 111(47.6%) subjects had different degrees of retinopathy. Bloating and early satiety and upper GI symptoms were higher in the subjects with retinopathy than another group. In summary, this study provides evidence that GI symptoms in diabetic subjects are independently linked to diabetic complications, particularly to retinopathy.
Dermatologic Manifestations in HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis
Authors: Boostani Reza , Layegh Pouran , Yazdanpanah Mohammad Javad , Vosoughi Elham , Mohammadi Azadeh , Joneidi Noseibe , Bazargani Reyhaneh , Sayadpour Zanjani Delaram , Ebrahimzadeh Saeed
Published: November 2019

Abstract- The human T cell lymphotropic virus type-1 (HTLV-1) is associated with adult T cell leukemia/lymphoma (ATL) and other disorders, including a slowly progressive demyelinating paraparesis, known as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Dermatologic manifestations are common in patients infected with HTLV-1 infection. In this study, we evaluated the dermatologic lesions associated with HAM/TSP patients in Mashhad, Iran. Dermatological findings of 37 patients with HAM/TSP were investigated and compared with those of an HTLV-1 negative control group. A cutaneous biopsy was performed as needed, and the results were statistically analyzed. Results of the present study showed that 34 cases with HAM/TSP (91.9%) and 24 cases in the control group (64.9%) had at least one skin lesion or history of skin lesion before (P=0.010). Xerosis was found in 22 persons (59.5%) in the case group and 4 persons in the control group (10.8%) (P=0.000). Only xerosis was significantly associated with HAM/TSP. Skin manifestations were quite frequent in patients with HAM/TSP. Xerosis was significantly associated with HAM/TSP
Epidemiology and Risk Factors of Cancer Among Children and Adolescents in Sulaimani City: A Case-Control Study
Authors: Mustafa Kamal Niaz , Othman Abdul Qadir Mardian , Ibrahim Hama Rahim Bestun , M. Ibrahem Abdulrahman , Karadagh Burhan
Published: November 2019

Abstract- Cancer can be defined as an abnormal growth of cells that can spread inside the body and affect many tissues and organs in different forms. It is a group of diseases that happen when abnormal cells grow and spread quickly. The purpose of the study was to determine the prevalent types of cancer and the risk factors associated with disease among children in Sulaimani city. The main part of this research was a hospital-based case-control study. The sample included 100 children aged 0-16 years (50 cases and 50 controls). The study was conducted in Sulaimani Hiwa Hospital and Pediatric Teaching Hospital from 1st January to 1st May 2018. Data on the exposed risk factors were obtained from face to face interview with the mother's cases and controls. Data was entered into Epidat version 3. Data were analyzed using STATA 11. Descriptive and percentages were used for Socio-demographic variables, odds ratios were used to determining potential risk factors, and P less and equal to 0.05 was estimated as statistically significant. The mean age of children was 46.2 (SD 42.7) months and the mean age of the mothers at the time of pregnancy was 28.4 (SD 6.7) years. Overall, there were 61 males and 39 females in the study. The commonest types were ALL (30%) while the less common types were osteosarcoma (4%). The risk factors linked to cancer were cesarean section (odds 2.7, P=0.02), family history (odds 10.8, P=0.008), mothers exposed to pesticides and chemicals during pregnancy (odds 3.0, P=0.01), (odds 6, P=0.0006) respectively, children exposed to pesticide (odds 5.4, P=0.02), exposed to passive smoke have similar odds (odds 3.0, P=0.01). Children's caffeine consumption (odds 2.3, P=0.04) and fast food (odds 3.0, P=0.01). In general, to control and prevent cancer among children, it is highlighting the need for communication and education mothers to aware about potential risk factors that lead to cancer. Furthermore, studies with larger sample size are required to determine other risk factors for cancer
Pleomorphic Ductal Carcinoma of Breast: Two Cases and Review Literature
Authors: Moghimi Mansour , Aryanfar Amir , Kargar Saeed , Joukar Fatemeh
Published: November 2019

Abstract- We report two cases of pleomorphic breast carcinoma, a rare variant of high-grade invasive ductal breast carcinoma of no special type, which is a combination of pleomorphic and bizarre giant cells in an adenocarcinoma background. For gaining better insights, available literature is also reviewed. © 2019 Tehran University of Medical Sciences. All rights reserved.
Two Rare Variants of Kaposi Sarcoma: Case Report
Authors: Darjani Abbas , Azimi Seyyede Zeinab , Mesbah Seyed Alireza
Published: November 2019

bstract- We reported two cases of immunocompetent patients with a rare form of AIDS-associated Kaposi sarcoma (KS), without visceral involvement, presenting with an unusual clinical and histopathological picture called telangiectatic and lymphangioma-like KS, respectively. Dermatologists and pathologists need to be aware of this uncommon described variant to avoid the potential for misdiagnosis.
A multi-centered study of Pneumocystis jirovecii colonization in patients with respiratory disorders: Is there a colonization trend in the elderly?
Authors: Abastabar Mahdi , Mosayebi Elham , Shokohi Tahereh , Hedayati1 Mohammad , Jabari Amiri Mohammad R. , Seifi Zahra , Haghani Iman , Aliyali Masoud , Saber Sassan , Sheikholeslami Maryam-Fatemeh
Published: December 2019

Background and Purpose: Pneumocystis jirovecii colonization plays a key role in the progression of pulmonary infection. However, there are limited data regarding the colonization of these fungi in the patients residing in different regions of Iran. Regarding this, the present study was conducted to evaluate the prevalence of P. jirovecii colonization in non-HIV-infected patients with respiratory failure introduced by physicians using nested polymerase chain reaction (PCR).
Materials and Methods: This study was conducted on 136 samples obtained from 136 patients with respiratory disorders referring to different hospitals in the capital and north of Iran during 2013-2015. The samples were collected using bronchoalveolar lavage (BAL; n=121) and sputum induction (n=15). Nested PCR method targeting mtLSU rRNA gene was used for the detection of P. jirovecii DNA in the specimens.
Results: The nested PCR analysis resulted in the detection of P. jirovecii DNA in 32 (23.5%) patients. The mean age of the participants was 49.04±11.94 years (age range: 14-90 years). The results revealed no correlation between Pneumocystis colonization and gender. The studied patients were divided into two groups of immunocompromised and immunocompetent patients. In the regard, 25.4% of the patients with detectable P. jirovecii DNA were immunocompromised and had cancer, organ transplantation, asthma, sarcoidosis, dermatomyositis, chronic obstructive pulmonary disease, bronchiectasis, and pulmonary vasculitis. On the other hand, Pneumocystis DNA was detected in 21.8% of the immunocompetent patients. Frequencies of P. jirovecii DNA detection in the patients with tuberculosis, hydatid cyst, and unknown underlying diseases were obtained as 20.8%, 25%, and 22%, respectively. The prevalence of Pneumocystis colonization varied based on age. In this regard, P. jirovecii colonization was more prevalent in patients aged above 70 years.
Conclusion: As the findings indicated, non-HIV-infected patients, especially the elderly, had a high prevalence of P. jirovecii colonization. Therefore, these patients are probably a potential source of infection for others. Regarding this, it is of paramount importance to adopt monitoring and prophylactic measures to reduce this infection.
The Synergistic Effect of Fluvastatin and IFN-λ on Peripheral Blood Mononuclear Cells of Chronic Hepatitis C Virus (HCV) Patients with IL-28B rs12979860 CC Genotype
Authors: Farzanegan Gharabolagh Amin , Bamdad Taravat , Hedayati Mehdi , Dehghan Manshadi Seyed Ali
Published: December 2019

There is a relationship between the life cycle of the hepatitis C virus (HCV) and the synthesis and hemostasis of lipids as well as lipid metabolism and interferon (IFN) regulatory system. This study aimed to examine the effect of fluvastatin and IFN-ƛ in the expression of mediators involved in lipid metabolism and HCV proliferation in patients with rs12979860 CC polymorphism.
Thirteen patients with HCV and five controls with rs1297986CC polymorphism were included in this study. Peripheral blood mononuclear cells (PBMCs) of patients and controls were treated by fluvastatin, IFN-λ or fluvastatin+IFN-λ. Assessment of IL-28B polymorphism, RNA extraction, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed. The mRNA expression of sterol regulatory element-binding protein 1 c (SREBP1c), ATP-binding cassette transporter A1 (ABCA1), diacylglycerol acyltransferase 1 (DGAT1), and HCV core as well as measurement of ABCA1 protein level were evaluated before and after treatment.
The results indicated that IFN-λ +fluvastatin acted as an inhibitor in mRNA expression of SREBP1c; while acting as an inducer in the expression of ABCA-1. The results of ABCA1 assay showed a significant increase of this protein after treatment with fluvastatin and IFN-λ compared with untreated cells (p=0.02). Moreover, the mRNA expression of HCV core was suppressed in all experimental groups treated with fluvastatin, IFN-λ or their combination which was more significant after treatment with fluvastatin+IFN-λ (p<0.001).
The results of this study demonstrated the significant effect of treatment with fluvastatin+IFN-λ in PBMCs of HCV patients with rs12979860 CC polymorphism. According to the drug resistance of viruses and prevention of virus-induced steatosis in patients with HCV, using regulatory agents of lipid mediators in parallel with current medications could be considered as an effective therapeutic strategy.
Anti-Varicella Zoster Virus IgG and hsCRP Levels Correlate with Progression of Coronary Artery Atherosclerosis
Authors: Fouladseresht Hamed , Ghamar Talepoor Atefe , Farjadian Shirin , Khosropanah Shahdad , Doroudchi Mehrnoosh
Published: December 2019

The relationship between high levels of anti-Varicella Zoster Virus (VZV) IgG in cerebrospinal fluid (CSF) and cerebrovascular atherosclerosis commends a possible similar association in other vessels. We aimed to investigate the association of VZV-seropositivity with coronary artery atherosclerosis.
We recruited 88 newly diagnosed patients with more than 50% stenosis in at least one of the main coronary arteries. As the control group, 99 age-matched individuals with normal/insignificant coronary artery findings were included. Clinical, paraclinical, and demographical data were gathered at the time of sampling. High‐sensitivity C‐reactive protein (hsCRP) levels were measured by nephelometry. VZV-seropositivity was determined by measuring of anti-VZV IgG level in plasma. Multivariable logistic regression was used to evaluate the correlation of data with coronary vascular atherosclerosis.
The frequency of VZV-seropositivity was significantly higher in the atherosclerosis group compared to the controls (OR=1.88; 95%CI=1.03-3.44). The plasma levels of anti-VZV IgG were significantly higher in patients with atherosclerosis (Median=2.70, IQR=1.53-4.30 AU/mL) than in the controls (Median=2.10, IQR=1.70-3.10 AU/mL, p=0.034). The hsCRP levels in patients and controls were 5.19±2.00 and 1.51±1.07 mg/L, respectively. The correlation between hsCRP and anti-VZV IgG level in plasma was observed (r=0.40, p<0.001). The levels of hsCRP and anti-VZV IgG increased based on the number of diseased vessels but only the difference in hsCRP levels reached a significant level (p<0.001 and p=0.168, respectively).
Our data suggest that VZV-seropositivity and hsCRP elevation jointly increase the risk of atherosclerosis. The multifactorial nature of atherosclerosis; however, leaves more options for the inflammatory milieu to be generated.
Outcome of Acute Deep Venous Thrombosis Using Standard Treatment versus Thrombolytics: A Literature Review
Authors: Birhan Abdella , Assefa Tamrat , Beyene Alemseged , Ndayishimiye Pacifique , Alebachew Woldu Minyahil
Published: December 2019

Deep vein thrombosis (DVT) is a major health problem affectinga significant portion of population. Primary complications are Pulmonary Embolism (PE) in the short term and Post-Thrombotic Syndrome (PTS) in the long term. Thrombolytic drugs act by activating plasminogen which in turn forms the enzyme plasmin. Plasmin consequently degrades blood clots by breaking down the fibrin molecules which make up the clots help to degrade the already formed clot. They can be used using different route of administration, doses and durations. The purpose of this systematic review was to assess the outcome of thrombolytic therapy in terms of the efficacy, safety and effectiveness of the medicines. Electronic searches of databases (MEDLINE and Google Scholar) were queried for articles written in English since 2000 GC. A total of 760 results were obtained using the search keys, and after excluding duplicates, 275 articles were selected. Finally, 9 randomized controlled trials (RCTs) which met the language of publication, study design and exclusion criteria were included in this systematic review. The data were obtained from nine trials (6 countries), providing a study-level data of 1309 participants. Almost all studies revealed that thrombolytic treatment was effective in the management of acute DVT. In most of the studies, the rate of rethrombosis was lower in case of thrombolytic than standard management. Hence, addition of thrombolytic results in persistence and increases the clinical benefits. Thrombolytic therapy was very effective in reversing closed veins, in boosting the patency rate,whilereflux was higher in patients treated with anticoagulants. Thrombolytic offers potential advantages over the standard treatment of DVT by reducing the proportion of patients with chronic disabling leg symptoms (such as PTS) by triple in the longer term. However, the incident of major bleeding was higher in patients receiving thrombolytics than anticoagulants.
A Two-Parameter Pranav Distribution with Properties and Its Application.
Authors: Umeh Edith , Ibenegbu Amuche
Published: December 2019

Introduction: Lifetime distribution has drawn so much attention in recent research, and this has lead to the development of new lifetime distribution. Addition of parameters to the existing distribution makes the distribution more flexible and reliable and applicable model has become the focus of the recent search. This paper proposed a two-parameter Pranav distribution which has its base from a one-parameter Pranav and Ishita distribution. Methods Two parameter Pranav distribution was proposed. Mathematical and statistical properties of the distribution which includes; moments, coefficient of variation, skewness, kurtosis, index of dispersion, hazard rate function, mean residual life function, stochastic ordering, mean deviation, Bonferroni and Lorenz curves were developed. Other lifetime distributions such as Ishita, Akash, Sujatha, Shanker, Lindley, and Exponential distributions were considered in the study. Results: This new distribution was compared with two-parameter Akash, Lindley, one parameter Pranav, Ishita, Akash, Sujatha, Shanker, Lindley, and Exponential distributions to determine the efficiency of the new model. The estimation of parameters has been X-rayed using the method of moments and maximum likelihood. Also, AIC and BIC were used to test for the goodness of fit of the model which was applied to a real-life data of hypertensive patients. The results show that the new two-parameter Pranav distribution has the lowest value of AIC and BIC Conclusion: Based on the AIC and BIC values we can conclude that the two-parameter Pranav is more efficient than the other distribution for modeling survival of hypertensive patients. Hence two-parameter Pranav can be seen as an important distribution in modeling lifetime data.
Potentiation Effect Of 5FU by Fragaceatoxin C Pore-Forming Toxin in MCF-7 Cell Line
Authors: Kheirandish Mohammad Hassan , Zarei Jaliani Hossein , Imani Mehdi
Published: December 2019

Introduction: Chemotherapy has been restricted due to the high-dose side effects. In the present study, acceleration of the chemotherapeutic drug (5FU) entrance into MCF-7 cells has been explored by using a recombinant form of Fragaceatoxin C (FraC) pore-forming toxin.
Methods: In this experimental study, the gene for FraC toxin was order from a commercial source and was sub-cloned into pET28a vector using NcoI and XhoI restriction enzymes. Expression of the protein was induced by IPTG and purification was performed using Ni-NTA affinity chromatography. Hemolysis was tested using a turbidimetric assay. Cytotoxic and potentiation effects of the recombinant toxin on 5FU chemotherapeutic drug were analyzed using trypan blue and MTT assays. T-student test was used to analyze the data using the SPSS v16 software. Significance was defined as P≤0.05
Results: Recombinant FraC toxin was produced in E. coli with 86% purity. Cytotoxic effects of the FraC toxin in MCF-7 cells were dose and time-dependent. the IC50 of FraC toxin on MCF-7 cells was 3.3 mg/ml. Non-toxic concentrations of recombinant FraC toxin enhanced cytotoxic effects of 5FU by a factor of 4 to 6 in different exposure times (24-72 h).
Conclusion: Potentiation of the 5FU chemotherapeutic effect by acceleration of its entrance into cancer target cells using FraC pore-forming toxin can reduce its side effects of the chemotherapy.
Renal Hydatid Cyst in a 50-year-old woman: A Case Report
Authors: Abdal Khadijeh , HafeziAhmadi Mohammad Reza
Published: December 2019

Introduction: Hydatidosis (hydatid cyst) is one of the most common infectious diseases between humans and animals that it is created by parasite (tapeworm) the echinococcusgranulosus larvae, which randomly infects an individual to one or more cysts. The hydatid cyst can affect all organs. The most common sites of involvement is liver and lung and rarely other organs, such as the kidneys, the brain, the heart, the bone and the spleen, can also be involved.
Due to the rare occurrence of renal hydatid cyst in this article, we report a 50 year old patient with renal hydatid cyst
THE SYMBOLIC SYSTEM IN TATAR POETRY OF THE FIRST HALF OF THE TWENTIETH CENTURY: THE TRANSFORMATION OF NOMINATIVE MEANINGS
Authors: F. Yusupov Ayrat , M. Yusupova Nurfiya , T. Sibgatullina Alfina
Published: November 2019

verifies the term of “nomination” in relation to poetic images-symbols. The interconnection of literary and cultural linguistics methods of analysis through the concept of nominations to the images-symbols made it possible to apply a new theoretical approach to the study of the symbolic system.

Methodology: in our study, we rely on the methodology of considering the symbol in the aspect of a system of nominations, which, in our opinion, expands the views on the process of symbolization.

Result: Thus, symbols characterize the national peculiarities of poets’ creativity and the artistic world of poetic works, the principles of organizing the subject level and the ideological and semantic potential of works. As a result, symbols have become a kind of “codes” that defines the unique and universal features of Tatar poetry.

Applications: This research can be used for universities, teachers, and students.

Novelty/Originality: In this research, the model of The Symbolic System in Tatar Poetry of the First Half of the Twentieth Century: The Transformation of Nominative Meanings is presented in a comprehensive and complete manner.
Configuración de subjetivaciones en pospenados en Colombia
Authors: Mendoza Molina Mónica , Bustos Benítez Paola
Published: December 2019

La configuración de subjetivaciones en el pospenado está atrave­sada de una u otra forma por su experiencia de prisión. Esta última varía en cada caso, al igual que los aspectos personales, culturales y sociales previos a la reclusión; sin embargo, se presentan sentimientos, actitudes y situaciones que les son comunes a los sujetos con esta condición. Los aspectos laboral, de familia y contextual, son escenarios siempre complejos e inciertos, que favorecen posibilidades de integración o reincidencia. El estigma se conso­lida como una barrera muy difícil de superar, considerando no solo aspectos sociales o familiares, sino también de tipo personal. Se busca con este artículo analizar algunos procesos de objetivación-subje­tivación que configuran al pospenado, tomando como base la información obtenida en campo a través de grupos focales, charlas y entrevistas, la cual fue contrastada con literatura especializada. Igualmente, se pretende ofrecer una concepción de un tipo particular de sujeto que busca ubicarse en ámbitos sociales, políticos y económicos, que de manera casi general, le marginan e invisibilizan.
The development and study of common wheat introgression lines derived from the synthetic form RS7
Authors: Davoyan R. O. , Bebyakina I. V. , Davoyan E. R. , Mikov D. S. , Zubanova Yu. S. , Boldakov D. M. , Badaeva E. D. , Adonina I. G. , Salina E. A. , Zinchenko A. N.
Published: November 2019

Synthetic recombination form RS7 (BBAAUS), in which the first two genomes, A and B, originate from common wheat, and the third recombinant genome consists of Aegilops speltoides (S) and Ae. umbellulata (U) chromosomes, was obtained from crossing synthetic forms Avrodes (BBAASS) and Avrolata (BBAAUU). Resistant to leaf rust, yellow rust and powdery mildew, introgression lines have been obtained from backcrosses with the susceptible varieties of common wheat Krasnodarskaya 99, Fisht and Rostislav. PCR analysis showed the presence of amplification fragments with marker SCS421 specific for the Lr28 gene in the line 4991n17. The cytological study (С-banding and FISH) of 14 lines has revealed chromosomal modifications in 12 of them. In most cases, the lines carry translocations from Ae. speltoides, which were identified in chromosomes 1D, 2D, 3D, 2B, 4B, 5B and 7B. Also, lines with the substituted chromosomes 1S (1B), 4D (4S), 5D (5S) and 7D (7S) were identified. Lines that have genetic material from Ae. speltoides and Ae umbellulata at once were revealed. In the line 3379n14, translocations in the short arm of chromosome 7D from Ae. umbellulata and chromosomes 5BL, 1DL, 2DL from Ae. speltoides were revealed. The line 4626p16 presumably has a translocation on the long arm of chromosome 2D from Ae. umbellulata and the T7SS.7SL-7DL translocation from Ae. speltoides. The T1DS.1DL-1SL and T3DS.3DL-3SL translocations from Ae. speltoides, and T2DS.2DL-2UL and T7DL.7DS-7US from Ae. umbellulata have been obtained for the first time. These lines may carry previously unidentified disease resistance genes and, in particular, leaf rust resistance genes from Ae. speltoides and Ae. umbellulata.
Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers
Authors: Sanamyan M. F. , Bobokhujayev Sh. U.
Published: November 2019

The lack of clear morphological markers of cotton chromosomes contributed to the development of an unconventional method for marking chromosomes using translocations. Today, tester translocation cotton lines represent the most complete set of cytological markers. The results of cytogenetic analysis of F1 hybrids obtained from crosses of monosomic cotton lines with translocation lines with identified chromosomes are presented. Cytogenetic identification and numbering of univalent chromosomes in 25 monosomic lines of the cytogenetic collection of the National University of Uzbekistan allowed us to establish the following univalent occurrences: chromosome 2 in four monosomic lines, chromosome 4 in 15 lines, chromosome 6 in four lines, chromosome 7 of the At-subgenome in one line and chromosome 18 of the Dt-subgenome in one line. The remaining 21 lines were duplicates of three non-homologous chromosomes. All monosomic lines identified were characterized by differences in univalent sizes, meiotic index, number of tetrads with micronuclei, pollen fertility, frequency of monosomy in the progeny, and a complex of morphological characters associated with the monosomy of the chromosome identified. Despite differences in the genotypic environment and methods for producing monosomics in the two cotton collections, there is a surprising coincidence of data suggesting a higher frequency of chromosomes 2, 4 and 6 occurring as monosomics, while the other chromosomes of the set occur as monosomics at a much lower frequency, and eight nonhomologous chromosomes (5, 8, 13 of the At-subgenome and 14, 15, 19, 22 and 24 of the Dt-subgenome of cotton) never do.
Endophytic Bacillus bacteria with RNase activity in the resistance of potato plants to viruses
Authors: Burkhanova G. F. , Sorokan A. V. , Cherepanova E. A. , Sarvarova E. R. , Khairullin R. M. , Maksimov I. V.
Published: November 2019

Viral diseases annually cause significant crop losses and significantly reduce the quality of products, including potatoes, some of the most important crops. Currently, viruses cannot be controlled with chemical pesticides, since known antiviral compounds are teratogenic and hazardous to people’s health. Biocontrol agents based on endophytic microorganisms may be an alternative to them. Many strains of Bacillus produce ribonucleases (RNases). Our laboratory possesses a collection of bacteria that produce various metabolites and have RNase activity. The results showed that the inoculation of potato with B. subtilis 26D and B. thuringiensis increased the grain yield by 32–43 %. In addition, the treatment of potato plants with Bacillus spp. significantly reduced the infection of potato plants with virus M. The prevalence of the disease in potato plants was significantly reduced from 60 % in the control to 18 % (B. subtillis 26D) and 25–33 % (B. thuringiensis) in the inoculated plants. Similarly, the infection index decreased from 14 in the control to 1 in the inoculated plants. The further study of molecular mechanisms related to bacterial induction of plant defense reactions in response to viral infections will lead to a better understanding of stress resistance problems. The endophytic microorganisms studied in this report may become the basis for the creation of biological agents for plant protection.
Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot
Authors: Kokhmetova A. M. , Atishova M. N. , Kumarbayeva M. T. , Leonova I. N.
Published: November 2019

Tan spot caused by the fungus Pyrenophora tritici-repentis is an important leaf spot disease in wheat growing areas throughout the world. The study aims to identify wheat germplasm resistant to tan spot based on phytopathological screening and molecular marker analysis. A collection of 64 common wheat germplasms, including cultivars and breeding lines from Kazakhstan and CIMMYT, was assessed for tan spot resistance in greenhouse conditions and characterized using the Xfcp623 molecular marker, diagnostic for the Tsn1 gene. All wheat cultivars/lines varied in their reaction to tan spot isolate race 1, ranging from susceptible to resistant. Most accessions studied (53 %) were susceptible to Ptr race 1. Spring wheat cultivars were more susceptible to race 1 than winter wheat cultivars. As a result of genotyping, an insensitive reaction to Ptr ToxA was predicted in 41 wheat cultivars (64 %). The tsn1 gene carriers identified included 27 Kazakhstani and 14 CIMMYT cultivars/lines, demonstrating insensitivity to Ptr ToxA. The majority of the Tsn1 genotype were sensitive to race 1 and showed susceptibility to the pathogen in the field. Disease scores from seedling stage positively correlated with field disease ratings. Of particular interest are 27 wheat accessions that demonstrated resistance to spore inoculation by Ptr race 1, were characterized by insensitivity to ToxA and showed field resistance to the pathogen. The results of this study will contribute to wheat breeding programs for tan spot resistance with Marker Assisted Selection using the closely flanking markers.
Identification of QTLs for resistance to leaf and stem rusts in bread wheat (Triticum aestivum L.) using a mapping population of ‘Pamyati Azieva × Paragon’
Authors: Genievskaya Y. , Fedorenko Y. , Sarbayev A. , Amalova A. , Abugalieva S. , Griffiths S. , Turuspekov Y.
Published: November 2019

Leaf rust (LR) and stem rust (SR) are harmful fungal diseases of bread wheat (Triticum aestivum L.). The purpose of this study was to identify QTLs for resistance to LR and SR that are effective in two wheat-growing regions of Kazakhstan. To accomplish this task, a population of recombinant inbred lines (RILs) of ‘Pamyati Azieva × Paragon’ was grown in the northern and southeastern parts of Kazakhstan, phenotyped for LR/SR severities, and analyzed for key yield components. The study revealed a negative correlation between disease severity and plant productivity in both areas. The mapping population was genotyped using a 20,000 Illumina SNP array. A total of 4595 polymorphic SNP markers were further selected for linkage analysis after filtering based on missing data percentage and segregation distortion. Windows QTL Cartographer was applied to identify QTLs associated with LR and SR resistances in the RIL mapping population studied. Two QTLs for LR resistance and eight for SR resistance were found in the north, and the genetic positions of eight of them have matched the positions of the known Lr and Sr genes, while two QTLs for SR were novel. In the southeast, eight QTLs for LR and one for SR were identified in total. The study is an initial step of the genetic mapping of LR and SR resistance loci of bread wheat in Kazakhstan. Field trials in two areas of the country and the genotyping of the selected mapping population have allowed identification of key QTLs that will be effective in regional breeding projects for better bread wheat productivity.
The formation and the study of a collection of the Miscanthus resource species gene pool in the conditions of the West Siberian forest steppe
Authors: Dorogina O. V. , Vasilyeva O. Yu. , Nuzhdina N. S. , Buglova I. V. , Zhmud E. V. , Zueva G. A. , Kominа O. V. , Kuban I. S. , Gusar A. S. , Dudkin R. V.
Published: November 2019

Several species of the genus Miscanthus Anderss. (elephant grass) characterized by a high rate of growth of the aboveground vegetative mass are currently in the focus of attention due to their high practical application as a source of bioethanol and cellulose. The main goals of this study were: (1) molecular genetic identification and (2) histochemical analysis of the genus Miscanthus Anderss. species in the collection of Central Siberian Botanical Garden SB RAS in order to identify the most perspective and technically valuable individuals. To study the collection of Miscanthus samples, a multi-disciplinary approach was applied. To collect the samples of different species from native habitats, traditional systematic and geobotanical methods (comparative morphological and phytocenological) were used. According to the results of the ISSR-analysis, 16 samples of three Miscanthus species were divided into two clades: Sinensis and Sacchariflorus, the former including two subclades. For the samples of M. purpurascens_I and II, a hybrid origin of this species was confirmed by ISSR data. The molecular data obtained from the study allowed us to hypothesize that the samples involved in the subclade I of the Sinensis clade could be used as donors of resistance to adverse environments, and the samples of the subclade II, as donors of high biomass productivity. Based on histochemical analysis, sclerenchyma cells were characterized by the most lignin-rich thickened membranes, so the most appropriate direction in Miscanthus selection should be based on identification and using less lignin-containing samples.
Erratum to: “The use of whole genome amplification for genomic evaluation of bovine embryos”
Authors: Pantiukh K. S. , Rukin I. V. , Portnov S. M. , Khatib A. , Panteleev S. L. , Mazur A. M.
Published: November 2019

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ANALISIS KESALAHAN MAHASISWA PENDIDIKAN MATEMATIKA DALAM MENYELESAIKAN SOAL GEOMETRI PADA TOPIK BANGUN RUANG [ERROR ANALYSIS OF STUDENTS IN THE MATHEMATICS DEPARTMENT IN SOLVING GEOMETRY PROBLEMS ON THE TOPIC OF SOLID FIGURES]
Authors: Listiani Tanti , Dirgantoro Kurnia P. S. , Saragih Melda J. , Tamba Kimura Patar
Published: December 2019

Geometry is a branch of science in mathematics and a course taken by students of mathematics education. Based on students' final exam scores, results were not optimal with several types of errors detected. This study aimed at identify student mistakes in solving geometry problems on the topic of solid figures. The subjects of this study were mathematics education students in the even semester of the 2018/2019 academic year at Universitas Pelita Harapan in Tangerang. The type of research was a qualitative descriptive study. The data collection technique used was a test. The results showed that students' errors in solving geometry problems were concept errors, calculation errors, and a lack of accuracy because they were in a hurry.

BAHASA INDONESIA ABSTRACT: Geometri merupakan salah satu cabang ilmu dalam matematika dan merupakan matakuliah yang wajib diikuti oleh mahasiswa program studi pendidikan matematika. Berdasarkan nilai ujian akhir semester mahasiswa didapatkan hasilnya masih kurang maksimal, hal ini memungkinkan terdapat beberapa tipe kesalahan jawaban dari mahasiswa. Penelitian ini bertujuan untuk mengidentifikasi kesalahan mahasiswa dalam menyelesaikan soal UAS geometri pada topik bangun ruang. Subjek penelitian ini adalah mahasiswa pendidikan matematika semester genap Universitas Pelita Harapan Tangerang tahun akademik 2018/2019. Jenis penelitian ini adalah penelitian deskriptif kualitatif. Teknik pengumpulan data yang digunakan adalah tes. Hasil penelitian menunjukkan bahwa kesalahan mahasiswa dalam menyelesaikan soal geometri adalah kesalahan konsep, kesalahan hitung, dan kurang teliti karena terburu-buru.
Letter to the Editor: Clinical Signs of Impending Death, A Retrospective Descriptive Analysis
Authors: Sobral Maria Ana , Runa Daniela , Julião Miguel
Published: December 2019

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"Museu Simeão Cananeia" - um espaço de história e educação em Bananeiras
Authors: Andrade Vivian Galdino de , Nascimento Rayane Cristina Ilário
Published: December 2019

Este artigo discute o Museu Simeão Cananéia como um lugar de educação em Bananeiras/PB. Fruto de uma pesquisa fundamentada na metodologia da educação patrimonial, expressa o desejo de registrar uma história, produzida a partir da relação do museu com a cidade. As memórias talhadas no acervo, inscritas no patrimônio arquitetônico e dadas a perceber pelas imagens e sons que ecoam durante as visitas que recebe foram elementos significativos para a produção desta escrita. O regimento da instituição, o decreto de criação, o inventário do acervo e o livro de assinaturas foram também consultados. Ao final desta investigação, constatamos que o museu carece de ações que potencializem suas possibilidades pedagógicas nas mais diversas áreas do saber, tanto como um veículo divulgador da história local, quanto como instituição que eduque e estimule o sentimento de preservação das coisas da cidade.
Habitat Fragmentation Effects on Community Composition of Carabid in Steppes of Northwest China
Authors: Wei Shu-hua , Huang Wen-guang , Zhu Meng-meng , Gao Li-yuan , Wang Ying , Zhang Rong , Li Zhi-hong , Zhao Zi-hua
Published: December 2019

It is well known that human activities and climate change have increased steppe habitat loss and fragmentation in Northwest China. Carabid beetles are often used as bioindicators of environmental change because they are extremely sensitive to disturbance. We chose 42 landscapes (18 fragmented and 24 continuous) in both desert and typical steppes of Northwest China to examine the influence of habitat loss and fragmentation on carabid beetle communities. The results showed the largest correlation coefficient between carabid communities and landscape compositions within a 7-km spatial scale in both desert and typical steppes. Further, the response of carabid communities to habitat fragmentation was species-specific in both desert and typical steppes. Habitat fragmentation in the desert steppe had positive effects on the richness and abundance of carabid communities, while in the typical steppe, the effects were negative. Additionally, habitat fragmentation significantly decreased the abundance of two common carabid species in the desert steppe. Therefore, the effects of habitat fragmentation on carabid biodiversity differ with species characteristics and habitat traits, where plant communities, soil structure, and microclimate vary in the different steppe types. The results of this study provide experimental evidence and technical support for biodiversity conservation management in the steppes of Northwest China.
PERANCANGAN MEDIA PEMBELAJARAN MENGGAMBAR TEKNIK DENGAN MENGGUNAKAN MACROMEDIA FLASH
Authors: Udin Muhhamad Nur , Setiawan Abdul Haris , Siswanto Budi
Published: November 2019

Penelitian ini betujuan untuk: (1) Merancang media pembelajaran menggambar teknik dengan menggunakan Macromedia Flash. (2) Mengetahui tingkat kelayakan media pembelajaran menggambar teknik dengan menggunakan Macromedia Flash. Metode penelitian yang digunakan dalam penelitian ini adalah metode riset dan pengembangan, dengan dilakukan perancangan media pembelajaran. Prosedur perancangan media pembelajaran dilakukan melalui: (1) Tahap studi pendahuluan dilakukan dengan studi literatur dan studi lapangan / observasi. (2) Tahap pengembangan dengan dilakukan pembuatan draft desain model / perancangan yang dibagi menjadi 3 bagian diantaranya: dilakukan penyusunan materi bersama dosen mata kuliah menggambar teknik, pembuatan naskah dan
storyboard, pengambilan video, editing video dan penyusunan dalam macromedia flash. Selanjutnya dilakukan validasi penilaian kepada para ahli / pakar materi, media dan pembelajaran untuk menilai kelayakan media pembelajaran yang dibuat. Selanjutnya dilakukan uji coba terbatas dengan 5 mahasiswa yang pernah menempuh mata kuliah menggambar teknik dan uji coba luas kepada 30 mahasiswa PTB FKIP UNS yang pernah menempuh mata kuliah menggambar teknik. (3) Tahap evaluasi merupakan tahap akhir dari proses perancangan terkait model final media pembelajaran menggambar teknik teknik.
 Hasil penelitian dari penilaian ahli materi menyatakan bahwa media pembelajaran menggambar teknik layak digunakan dengan persentase sebesar 73%. Penilaian ahli media dengan persentase 91 % menyatakan bahwa perancangan media pembelajaran menggambar teknik termasuk kategori sangat layak. Penilaian ahli pembelajaran menyatakan bahwa perancangan media pembelajaran menggambar teknik layak dengan persentase sebesar 67 % sedangkan uji coba terbatas hasil yang diperoleh sebesar 84 % menyatakan bahwa media pembelajaran yang dirancang sangat layak kemudian pada uji coba luas menyatakan dengan
persentase 81% bahwa media pembelajaran termasuk kategori sangat layak.