bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Graphene Oxide on Zero Shear Viscosity, Fatigue Life and Low-Temperature Properties of Asphalt Binder
Authors: Adnan Abbas Mukhtar , Lü Chaofeng , Luo Xue , Wang Jinchang
Published: June 2021

This study has investigated the impact of graphene oxide (GO) in enhancing the performance properties of an asphalt binder. The control asphalt binder (60/70 PEN) was blended with GO in contents of 0%, 0.5%, 1%, 1.5%, 2%, and 2.5%. The permanent deformation behavior of the modified asphalt binders was evaluated based on the zero shear viscosity (ZSV) parameter through a steady shear test approach. Superpave fatigue test and the linear amplitude sweep (LAS) method were used to evaluate the fatigue behavior of the binders. A bending beam rheometer (BBR) test was conducted to evaluate the low-temperature cracking behavior. Furthermore, the storage stability of the binders was investigated using a separation test. The results of the ZSV test showed that GO considerably enhanced the steady shear viscosity and ZSV value, showing a significant contribution of the GO to the deformation resistance; moreover, GO modification changed the asphalt binder’s behavior from Newtonian to shear-thinning flow. A notable improvement in fatigue life was observed with the addition of GO to the binder based on the LAS test results and Superpave fatigue parameter. The BBR test results revealed that compared to the control asphalt, the GO-modified binders showed lower creep stiffness (S) and higher creep rate (m-value), indicating increased cracking resistance at low temperatures. Finally, the GO-modified asphalt binders exhibited good storage stability under high temperatures.
Influence of Test Conditions on Sliding Wear Performance of High Velocity Air Fuel-Sprayed WC–CoCr Coatings
Authors: Torkashvand Kaveh , Selpol Vinod Krishna , Gupta Mohit , Joshi Shrikant
Published: June 2021

Sliding wear performance of thermal spray WC-based coatings has been widely studied. However, there is no systematic investigation on the influence of test conditions on wear behaviour of these coatings. In order to have a good understanding of the effect of test parameters on sliding wear test performance of HVAF-sprayed WC–CoCr coatings, ball-on-disc tests were conducted under varying test conditions, including different angular velocities, loads and sliding distances. Under normal load of 20 N and sliding distance of 5 km (used as ‘reference’ conditions), it was shown that, despite changes in angular velocity (from 1333 rpm up to 2400 rpm), specific wear rate values experienced no major variation. No major change was observed in specific wear rate values even upon increasing the load from 20 N to 40 N and sliding distance from 5 km to 10 km, and no significant change was noted in the prevailing wear mechanism, either. Results suggest that no dramatic changes in applicable wear regime occur over the window of test parameters investigated. Consequently, the findings of this study inspire confidence in utilizing test conditions within the above range to rank different WC-based coatings.
Effects of the Interface between Inorganic and Organic Components in a Bi2Te3–Polypyrrole Bulk Composite on Its Thermoelectric Performance
Authors: Kim Cham , Lopez David Humberto
Published: June 2021

We provided a method to hybridize Bi2Te3 with polypyrrole, thus forming an inorganic/organic bulk composite (Bi2Te3–polypyrrole), in which the effects of energy band junction and phonon scattering were expected to occur at the interface of the two components. Bi2Te3–polypyrrole exhibited a considerably high Seebeck coefficient compared to pristine Bi2Te3, and thus it recorded a somewhat increased power factor despite the loss in electrical conductivity caused by the organic component, polypyrrole. Bi2Te3–polypyrrole also exhibited much lower thermal conductivity than pristine Bi2Te3 because of the phonon scattering effect at the interface. We successfully brought about the decoupling phenomenon of electrical and thermal properties by devising an inorganic/organic composite and adjusting its fabrication condition, thereby optimizing its thermoelectric performance, which is considered the predominant property for n-type binary Bi2Te3 reported so far.
Test and Numerical Simulation Investigation on Seismic Performance of Different Types of Expansive Polystyrene Granule Cement Latticed Concrete Walls
Authors: Cao Xinyu , Li Xiaojun , Tang Baizan
Published: June 2021

An expansive polystyrene granule cement (EPSC) latticed concrete wall with diagonal bracing is formed with a traditional EPSC latticed concrete wall skeleton with added diagonal bracing. It is a new model of non-demolding wall integrating insulation and structure. For the new model, the length of one EPSC panel is 1200 mm, which is 300 mm longer than that of the traditional one. The diagonal bracing is arranged in a 45° orthogonal grid in the new model. In contrast, the traditional type has only horizontal lattice beams and vertical lattice columns. Through the pseudo-static test of two new EPSC latticed concrete wall specimens with diagonal bracing and two traditional EPSC latticed concrete wall specimens, the seismic performance of latticed concrete walls was investigated in this study. The main difference between the specimens was the lattice form and the core hole diameter. Finite element simulation was carried out on the simplified models of a latticed concrete wall with diagonal bracing. The results showed that EPSC could work with post-poured concrete to withstand earthquake action together. Additionally, the lateral performance of the EPSC latticed concrete wall with diagonal bracing was significantly improved compared with the traditional type, and the overall seismic performance was improved, especially the energy dissipation capacity, which increased by more than 180%. The bearing capacity increased by more than 12%, when the amount of concrete was basically the same. The initial stiffness was improved by more than 52%. As the diameter of the core hole increased 20 mm, the bearing capacity improved more than 12%. Simplified modeling methods could be used to analyze the seismic performance of latticed concrete walls under lateral cyclic loading. The study reveals the seismic performance characteristics of latticed composite walls with different lattice forms and core hole diameters, and it provides technical support for the engineering application of different lattice forms and core hole diameter latticed composite walls.
Effective Singlet Oxygen Sensitizers Based on the Phenazine Skeleton as Efficient Light Absorbers in Dye Photoinitiating Systems for Radical Polymerization of Acrylates
Authors: Pyszka Ilona , Kucybała Zdzisław , Jędrzejewska Beata
Published: June 2021

A series of dyes based on the phenazine skeleton were synthesized. They differed in the number of conjugated double bonds, the arrangement of aromatic rings (linear and/or angular system), as well as the number and position of nitrogen atoms in the molecule. These compounds were investigated as potential singlet oxygen sensitizers and visible light absorbers in dye photoinitiating systems for radical polymerization. The quantum yield of the singlet oxygen formation was determined by the comparative method based on the 1H NMR spectra recorded for the tested dyes in the presence of 2,3-diphenyl-p-dioxene before and after irradiation. The quantum yield of the triplet state formation was estimated based on the transient absorption spectra recorded using the nanosecond flash photolysis technique. The effectiveness of the dye photoinitiating system was characterized by the initial rate of trimethylolpropane triacrylate (TMPTA) polymerization. In the investigated photoinitiating systems, the sensitizer was an electron acceptor, whereas the co-initiator was an electron donor. The effectiveness of TMPTA photoinitiated polymerization clearly depended on the arrangement of aromatic rings and the number of nitrogen atoms in the modified phenazine structure as well as the quantum yield of the triplet state formation of the photosensitizer in the visible light region.
Study on Performance Damage and Mechanism Analysis of Asphalt under Action of Chloride Salt Erosion
Authors: Zhou Peilei , Wang Wensheng , Zhu Lili , Wang Haoyun , Ai Yongming
Published: June 2021

This study aims to investigate the performance evolution and mechanism of asphalt under action of chloride salt erosion. Asphalt samples soaked with five different snow melting chloride salt concentrations were taken as the research object. Then, the high-temperature performance, low-temperature performance, temperature sensitivity and asphalt–aggregate adhesion property of asphalt samples were carried out. Additionally, Fourier transform infrared spectroscopy (FTIR) was used to explore the mechanism of chloride salt erosion on asphalt. Test results showed the linear variation relationships of high-temperature performance, low-temperature performance and temperature sensitivity with chloride salt concentrations. The high-temperature performance of asphalt would be improved by chloride snowmelt salt. With the increase in the chloride salt solution concentration, the low-temperature performance of asphalt became worse, and the temperature sensitivity increased. Moreover, after the effect of the chloride salt solution, the asphalt–aggregate adhesion property decreased with the increase in the chloride salt solution concentration. It is necessary to control the amount of chloride snowmelt salt in the actual snow removal projects. Finally, based on Fourier transform infrared spectroscopy, the mechanism of chloride salt erosion on asphalt was preliminarily explored. With the increase in the chloride salt solution concentration, the proportion of light components (saturated fraction, aromatic fraction) in asphalt decreased, and the proportion of heavy components (resin and asphaltene) with good thermal stability increased.
Outperformance in Acrylation: Supported D-Glucose-Based Ionic Liquid Phase on MWCNTs for Immobilized Lipase B from Candida antarctica as Catalytic System
Authors: Szelwicka Anna , Erfurt Karol , Jurczyk Sebastian , Boncel Slawomir , Chrobok Anna
Published: June 2021

This study presents a highly efficient method of a synthesis of n-butyl acrylate via esterification of acrylic acid and n-butanol in the presence of supported ionic liquid phase (SILP) biocatalyst consisting of the lipase B from Candida antarctica (CALB) and multi-walled carbon nanotubes (MWCNTs) modified by D-glucose-based ionic liquids. Favorable reaction conditions (acrylic acid: n-butanol molar ratio 1:2, cyclohexane as a solvent, biocatalyst 0.150 g per 1 mmol of acrylic acid, temperature 25 °C) allowed the achievement of a 99% yield of n-butyl acrylate in 24 h. Screening of various ionic liquids showed that the most promising result was obtained if N-(6-deoxy-1-O-methoxy-α-D-glucopyranosyl)-N,N,N-trimethylammonium bis-(trifluoromethylsulfonyl)imide ([N(CH3)3GlcOCH3][N(Tf)2]) was selected in order to modify the outer surface of MWCNTs. The final SILP biocatalyst–CNTs-[N(CH3)3GlcOCH3][N(Tf)2]-CALB contained 1.8 wt.% of IL and 4.2 wt.% of CALB. Application of the SILP biocatalyst led to the enhanced activity of CALB in comparison with the biocatalyst prepared via physical adsorption of CALB onto MWCNTs (CNTs-CALB), as well as with commercially available Novozyme 435. Thus, the crucial role of IL in the stabilization of biocatalysts was clearly demonstrated. In addition, a significant stability of the developed biocatalytic system was confirmed (three runs with a yield of ester over 90%).
Effect of the Chloro-Substitution on Electrochemical and Optical Properties of New Carbazole Dyes
Authors: Krawczyk Przemysław , Jędrzejewska Beata , Seklecka Klaudia , Cytarska Joanna , Łączkowski Krzysztof Z.
Published: June 2021

Carbazole derivatives are the structural key of many biologically active substances, including naturally occurring and synthetic ones. Three novel (E)-2-(2-(4-9H-carbazol-9-yl)benzylidene)hydrazinyl)triazole dyes were synthesized with different numbers of chlorine substituents attached at different locations. The presented research has shown the influence of the number and position of attachment of chlorine substituents on electrochemical, optical, nonlinear, and biological properties. The study also included the analysis of the use of the presented derivatives as potential fluorescent probes for in vivo and in vitro tests. Quantum-chemical calculations complement the conducted experiments.
A Perov Version of Fuzzy Metric Spaces and Common Fixed Points for Compatible Mappings
Authors: Martínez-Moreno Juan , Gopal Dhananjay
Published: June 2021

In this paper, we define and study the Perov fuzzy metric space and the topology induced by this space. We prove Banach contraction theorems. Moreover, we devised new results for Kramosil and Michálek fuzzy metric spaces. In the process, some results about multidimensional common fixed points as coupled/tripled common fixed point results are derived from our main results.
Identifying the Maximum Concentration of Results in Bilateral Sports Competitions
Authors: Avila-Cano Antonio , Ruiz-Sepulveda Amparo , Triguero-Ruiz Francisco
Published: June 2021

There are situations in which a monopoly solution cannot be reached. In these cases, which situation represents the maximum concentration (minimum competitive balance)? It is a relevant question, given that in sports economics, measuring the competitive balance of a league is done through normalized indices. These indices require that the maximum level of concentration be known. Until now, the distribution of results that generates the maximum level of concentration has been identified in the literature as Complete cascade distribution. However, if the scoring system used does not fulfil the stability condition, which implies that the total number of points of a championship is constant, it can be demonstrated that the Complete cascade distribution does not generate the maximum level of concentration. This is the case, for example, with major European football leagues. In this article, we constructed a perfectly unbalanced distribution, which we called a Truncated cascade distribution. This distribution generates the maximum concentration level. Therefore, if we do not use Truncated cascade distribution, there is an overestimation of the concentration measured with normalized indices. Then, the calculated competitive balance will be wrong, that is, underestimated. We provided a spreadsheet for identifying this distribution.
Masked Implementation of Format Preserving Encryption on Low-End AVR Microcontrollers and High-End ARM Processors
Authors: Kim Hyunjun , Sim Minjoo , Jang Kyoungbae , Kwon Hyeokdong , Uhm Siwoo , Seo Hwajeong
Published: June 2021

Format-Preserving Encryption (FPE) for Internet of Things (IoT) enables the data encryption while preserving the format and length of original data. With these advantages, FPE can be utilized in many IoT applications. However, FPE requires complicated computations and these are high overheads on IoT embedded devices. In this paper, we proposed an efficient implementation of Format-preserving Encryption Algorithm (FEA), which is the Korean standard of FPE, and the first-order masked implementation of FEA on both low-end (i.e., AVR microcontroller) and high-end (i.e., ARM processor) IoT devices. Firstly, we show the vulnerability of FEA when it comes to the Correlation Power Analysis (CPA) approach. Afterward, we propose an efficient implementation method and the masking technique for both low-end IoT device and high-end IoT device. The proposed method is secure against power analysis attacks but the performance degradation of masked measure is only 2.53∼3.77% than the naïve FEA implementation.
Vector Geometric Algebra in Power Systems: An Updated Formulation of Apparent Power under Non-Sinusoidal Conditions
Authors: Montoya Francisco G. , Baños Raúl , Alcayde Alfredo , Arrabal-Campos Francisco Manuel , Roldán-Pérez Javier
Published: June 2021

Traditional electrical power theories and one of their most important concepts—apparent power—are still a source of debate, because they present several flaws that misinterpret the power-transfer and energy-balance phenomena under distorted grid conditions. In recent years, advanced mathematical tools such as geometric algebra (GA) have been introduced to address these issues. However, the application of GA to electrical circuits requires more consensus, improvements and refinement. In this paper, electrical power theories for single-phase systems based on GA were revisited. Several drawbacks and inconsistencies of previous works were identified, and some amendments were introduced. An alternative expression is presented for the electric power in the geometric domain. Its norm is compatible with the traditional apparent power defined as the product of the RMS voltage and current. The use of this expression simplifies calculations such as those required for current decomposition. This proposal is valid even for distorted currents and voltages. Concepts are presented in a simple way so that a strong background on GA is not required. The paper included some examples and experimental results in which measurements from a utility supply were analysed.
Study of an Environmentally Friendly Method for the Dissolution of Precious Metal with Ionic Liquid and Iodoalkane
Authors: Feng Feng , Liu Jiamei , Zhao Mingxing , Yu Lu , Wang Haixin , Lu Chunshan , Zhang Qunfeng , Zhao Jia , Sun Yanxia , Cen Jie , Li Xiaonian
Published: June 2021

Gold as a precious metal resource has high recycling significance. However, the current extraction methods cannot achieve the both efficiency and environmental friendliness. In this paper, we propose a new gold leaching agent, which can leach gold under light condition by mixing iodoform (CHI3) with 1-butyl-3-methylimidazolium dicyanamide (BmimN(CN)2) ionic liquid. Under 25 °C and 13 W incandescent lamp irradiation, the leaching yield of gold can achieve 100 wt%, and the average leaching rate is 945 mg Au/(h·mol·CHI3) (18.9 times of that of the cyanidation method). Through the analysis of the results of radical inhibition experiment, UV-Vis and XPS, a possible leaching mechanism is proposed: the iodine radical generated by light oxidizes Au0 to Au+, and then forms AuN(CN)2 by coordinating with N(CN)2−. Subsequently, the ionic liquid and Au N(CN)2 form a stable [Bmim]·[Au(N(CN)2)2] ion pair structure, further promoting the dissolution reaction. The leaching yield of gold can reach 81.9 wt% and 100 wt%, respectively, when applied to ore and waste electrical and electronic equipment (WEEE); the leaching yield of gold can also reach 100 wt% when applied to a waste catalyst by adding a Soxhlet extraction. The results show that this method is not only efficient, mild, and environmentally friendly, but also has strong adaptability and wide application prospects.
Damage Effect of ALD-Al2O3 Based Metal-Oxide-Semiconductor Structures under Gamma-Ray Irradiation
Authors: Ding Man
Published: June 2021

The radiation response of Al2O3 on silicon substrate under gamma-rays is studied in this article. The atomic layer deposited Al2O3 based metal-oxide-semiconductor structures were irradiated under gamma-ray with the total dose of 1.2 Mrad(Si)/2.5 Mrad(Si)/4 Mrad(Si). The generation, transportation and trapping characteristics of radiation induced charges were studied by using electronic, physical and chemical methods. Firstly, the radiation induced trapped charge density in Al2O3 is up to 1012 cm−2, with the effective trapping efficiency of 7–20% under irradiation. Secondly, the leakage current through Al2O3 changes little with the increase of radiation total dose. Thirdly, oxygen vacancy in Al2O3 and O dangling bonds and Al-Si metallic bonds at Al2O3/Si interface are dominant radiation induced defects in Al2O3/Si system, and the valence band offset between Al2O3 and Si is found to decrease after irradiation. From the results we can see that Al2O3 is radiation resistant from the aspect of leakage current and crystallization characteristics, but the radiation induced charge trapping and new defects in Al2O3/Si structure cannot be ignored. This paper provides a reference for the space application of Al2O3 based MOS devices.
Gut Microbiota Dynamics in Natural Populations of Pintomyia evansi under Experimental Infection with Leishmania infantum
Authors: Vivero Rafael José , Castañeda-Monsalve Victor Alfonso , Romero Luis Roberto , D. Hurst Gregory , Cadavid-Restrepo Gloria , Moreno-Herrera Claudia Ximena
Published: June 2021

Pintomyia evansi is recognized by its vectorial competence in the transmission of parasites that cause fatal visceral leishmaniasis in rural and urban environments of the Caribbean coast of Colombia. The effect on and the variation of the gut microbiota in female P. evansi infected with Leishmania infantum were evaluated under experimental conditions using 16S rRNA Illumina MiSeq sequencing. In the coinfection assay with L. infantum, 96.8% of the midgut microbial population was composed mainly of Proteobacteria (71.0%), followed by Cyanobacteria (20.4%), Actinobacteria (2.7%), and Firmicutes (2.7%). In insect controls (uninfected with L. infantum) that were treated or not with antibiotics, Ralstonia was reported to have high relative abundance (55.1–64.8%), in contrast to guts with a high load of infection from L. infantum (23.4–35.9%). ASVs that moderately increased in guts infected with Leishmania were Bacillus and Aeromonas. Kruskal–Wallis nonparametric variance statistical inference showed statistically significant intergroup differences in the guts of P. evansi infected and uninfected with L. infantum (p < 0.05), suggesting that some individuals of the microbiota could induce or restrict Leishmania infection. This assay also showed a negative effect of the antibiotic treatment and L. infantum infection on the gut microbiota diversity. Endosymbionts, such as Microsporidia infections (<2%), were more often associated with guts without Leishmania infection, whereas Arsenophonus was only found in guts with a high load of Leishmania infection and treated with antibiotics. Finally, this is the first report that showed the potential role of intestinal microbiota in natural populations of P. evansi in susceptibility to L. infantum infection.
Cellular Immune Response Induced by DNA Immunization of Mice with Drug Resistant Integrases of HIV-1 Clade A Offers Partial Protection against Growth and Metastatic Activity of Integrase-Expressing Adenocarcinoma Cells
Authors: Isaguliants Maria , Krotova Olga , Petkov Stefan , Jansons Juris , Bayurova Ekaterina , Mezale Dzeina , Fridrihsone Ilze , Kilpelainen Athina , Podschwadt Philip , Agapkina Yulia , Smirnova Olga , Kostic Linda , Saleem Mina , Latyshev Oleg , Eliseeva Olesja , Malkova Anastasia , Gorodnicheva Tatiana , Wahren Britta , Gordeychuk Ilya , Starodubova Elizaveta , Latanova Anastasia
Published: June 2021

Therapeutic DNA-vaccination against drug-resistant HIV-1 may hinder emergence and spread of drug-resistant HIV-1, allowing for longer successful antiretroviral treatment (ART) up-to relief of ART. We designed DNA-vaccines against drug-resistant HIV-1 based on consensus clade A integrase (IN) resistant to raltegravir: IN_in_r1 (L74M/E92Q/V151I/N155H/G163R) or IN_in_r2 (E138K/G140S/Q148K) carrying D64V abrogating IN activity. INs, overexpressed in mammalian cells from synthetic genes, were assessed for stability, route of proteolytic degradation, and ability to induce oxidative stress. Both were found safe in immunotoxicity tests in mice, with no inherent carcinogenicity: their expression did not enhance tumorigenic or metastatic potential of adenocarcinoma 4T1 cells. DNA-immunization of mice with INs induced potent multicytokine T-cell response mainly against aa 209–239, and moderate IgG response cross-recognizing diverse IN variants. DNA-immunization with IN_in_r1 protected 60% of mice from challenge with 4Tlluc2 cells expressing non-mutated IN, while DNA-immunization with IN_in_r2 protected only 20% of mice, although tumor cells expressed IN matching the immunogen. Tumor size inversely correlated with IN-specific IFN-γ/IL-2 T-cell response. IN-expressing tumors displayed compromised metastatic activity restricted to lungs with reduced metastases size. Protective potential of IN immunogens relied on their immunogenicity for CD8+ T-cells, dependent on proteasomal processing and low level of oxidative stress.
Characterization and Analysis of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in Pandemic and Non-Pandemic Vibrio parahaemolyticus Isolates from Seafood Sources
Authors: Jingjit Nawaporn , Preeprem Sutima , Surachat Komwit , Mittraparp-arthorn Pimonsri
Published: June 2021

Vibrio parahaemolyticus is one of the significant seafood-borne pathogens causing gastroenteritis in humans. Clustered regularly interspaced short palindromic repeats (CRISPR) are commonly detected in the genomes of V. parahaemolyticus and the polymorphism of CRISPR patterns has been applied as a genetic marker for tracking its evolution. In this work, a total of 15 pandemic and 36 non-pandemic V. parahaemolyticus isolates obtained from seafood between 2000 and 2012 were characterized based on hemolytic activity, antimicrobial susceptibility, and CRISPR elements. The results showed that 15/17 of the V. parahaemolyticus seafood isolates carrying the thermostable direct hemolysin gene (tdh+) were Kanagawa phenomenon (KP) positive. The Multiple Antibiotic Resistance (MAR) index ranged between 0.1 and 0.4, and 45% of the isolates have an MAR index ≥ 0.2. A total of 19 isolates were positive for CRISPR detection, including all tdh+ trh− isolates, two of tdh− trh+, and each of tdh+ trh+ and tdh− trh−. Four spacer types (Sp1 to Sp4) were identified, and CRISPR-positive isolates had at least one type of spacer homolog to the region of Vibrio alginolyticus megaplasmid. It is of interest that a specific CRISPR profile and spacer sequence type was observed with correlations to the hemolysin genotype (tdh/trh). Thus, these provide essential data on the exposure of foreign genetic elements and indicate shared ancestry within different genotypes of V. parahaemolyticus isolates.
A Large Case Series of Neurocysticercosis in Kuwait, a Nonendemic Arabian Gulf Country in the Middle East Region
Authors: Iqbal Jamshaid , Ahmad Suhail , Al-Awadhi Mohammad , Masud Amir , Mohsin Zainab , Abdulrasoul Abdullah Y. , Albenwan Khalifa , Alenezi Nadia , AlFarsi Fatima
Published: June 2021

Neurocysticercosis (NCC), a leading global cause of severe progressive headache and epilepsy, in developed or affluent countries is mostly diagnosed among immigrants from poor or developing Taenia solium taeniasis-endemic countries. Taeniasis carriers in Kuwait are routinely screened by insensitive stool microscopy. In this study, enzyme-linked immunoelectrotransfer blot (EITB) was used as a confirmatory test for NCC. Screening was performed on 970 patients referred for suspected NCC on the basis of relevant history and/or ring-enhancing lesions on computed tomography and/or magnetic resonance imaging during a 14-year period in Kuwait. Demographic data and clinical details were retrieved from laboratory or hospital records. EITB was positive in 150 subjects (15.5%), including 98 expatriates mostly originating from taeniasis-endemic countries and, surprisingly, 52 Kuwaiti nationals. The clinical details of 48 of 50 NCC cases diagnosed during 2014–2019 were available. Most common symptoms included seizures, persistent headache with/without fever, and fits or loss of consciousness. Cysticercal lesions were located at various brain regions in 39 of 48 patients. Multiple members of 3 families with NCC were identified; infection was linked to domestic workers from taeniasis-endemic countries and confirmed in at least 1 family. Our data show that NCC is predominantly imported in Kuwait by expatriates originating from taeniasis-endemic countries who transmit the infection to Kuwaiti citizens.
Probiotic Potential and Cholesterol-Lowering Capabilities of Bacterial Strains Isolated from Pericarpium Citri Reticulatae ‘Chachiensis’
Authors: He Qianxian , Li Jingyu , Ma Yongkai , Chen Qi , Chen Gu
Published: June 2021

Pericarpium Citri Reticulatae ‘Chachiensis’ (PCR-Chachiensis), the pericarps of Citri Reticulatae Blanco cv. Chachiensis, is a food condiment and traditional medicine in southeast and eastern Asia. Its rich and various bacterial community awaits exploration. The present study is the first report on probiotic screening and characterization of bacteria from PCR-Chachiensis. Based on 64 culturable bacterial isolates, 8 strains were screened out to have great survival in the simulated gastrointestinal stressful condition, being nonhemolytic and without biogenic amine formation. They were identified by 16S rRNA gene sequencing as two Bacillus, three Lactobacillus, and three strains from Bacillales. Their probiotic properties, cholesterol-lowering potential and carbohydrate utilization capability were further investigated. Though these eight strains all displayed distinct cholesterol removal potential, Bacillus licheniformis N17-02 showed both remarkable cholesterol removal capability and presence of bile salt hydrolase gene, as well as possessing most of the desirable probiotic attributes. Thus, it could be a good probiotic candidate with hypocholesterolemic potential. Bacillus megaterium N17-12 displayed the widest carbohydrate utilization profile and the strongest antimicrobial activity. Hence, it was promising to be used as a probiotic in a host and as a fermentation starter in fermented food or feed.
Diversity in Chemical Structures and Biological Properties of Plant Alkaloids
Authors: Bhambhani Sweta , Kondhare Kirtikumar R. , Giri Ashok P.
Published: June 2021

Phytochemicals belonging to the group of alkaloids are signature specialized metabolites endowed with countless biological activities. Plants are armored with these naturally produced nitrogenous compounds to combat numerous challenging environmental stress conditions. Traditional and modern healthcare systems have harnessed the potential of these organic compounds for the treatment of many ailments. Various chemical entities (functional groups) attached to the central moiety are responsible for their diverse range of biological properties. The development of the characterization of these plant metabolites and the enzymes involved in their biosynthesis is of an utmost priority to deliver enhanced advantages in terms of biological properties and productivity. Further, the incorporation of whole/partial metabolic pathways in the heterologous system and/or the overexpression of biosynthetic steps in homologous systems have both become alternative and lucrative methods over chemical synthesis in recent times. Moreover, in-depth research on alkaloid biosynthetic pathways has revealed numerous chemical modifications that occur during alkaloidal conversions. These chemical reactions involve glycosylation, acylation, reduction, oxidation, and methylation steps, and they are usually responsible for conferring the biological activities possessed by alkaloids. In this review, we aim to discuss the alkaloidal group of plant specialized metabolites and their brief classification covering major categories. We also emphasize the diversity in the basic structures of plant alkaloids arising through enzymatically catalyzed structural modifications in certain plant species, as well as their emerging diverse biological activities. The role of alkaloids in plant defense and their mechanisms of action are also briefly discussed. Moreover, the commercial utilization of plant alkaloids in the marketplace displaying various applications has been enumerated.
Anticorrosive and Microbial Inhibition Performance of a Coating Loaded with Andrographis paniculata on Stainless Steel in Seawater
Authors: Kamaruzzaman Wan Mohamad Ikhmal Wan Mohamad , Fekeri Maria Fazira Mohd , Nasir Nursabrina Amirah Mohd , Hamidi Nur Aiman Syafiq Mohd , Baharom Mohamad Zahid , Adnan Azila , Shaifudin Muhamad Syaizwadi , Abdullah Wan Rafizah Wan , Wan Nik Wan Mohd Norsani , Suhailin Fariza Hanim , Matori Khamirul Amin , Kien Chen Soo , Zaid Mohd Hafiz Mohd , Ghazali Mohd Sabri Mohd
Published: June 2021

With the trend for green technology, the study focused on utilizing a forgotten herb to produce an eco-friendly coating. Andrographis paniculata or the kalmegh leaves extract (KLE) has been investigated for its abilities in retarding the corrosion process due to its excellent anti-oxidative and antimicrobial properties. Here, KLE was employed as a novel additive in coatings and formulations were made by varying its wt%: 0, 3, 6, 9, and 12. These were applied to stainless steel 316L immersed in seawater for up to 50 days. The samples were characterized and analyzed to measure effectiveness of inhibition of corrosion and microbial growth. The best concentration was revealed to be 6 wt% KLE; it exhibited the highest performance in improving the ionic resistance of the coating and reducing the growth of bacteria.
A New Chalcone Derivative with Promising Antiproliferative and Anti-Invasion Activities in Glioblastoma Cells
Authors: Mendanha Daniel , Vieira de Castro Joana , Moreira Joana , Costa Bruno M. , Cidade Honorina , Pinto Madalena , Ferreira Helena , Neves Nuno M.
Published: June 2021

Glioblastoma (GBM) is the most common and most deadly primary malignant brain tumor. Current therapies are not effective, the average survival of GBM patients after diagnosis being limited to few months. Therefore, the discovery of new treatments for this highly aggressive brain cancer is urgently needed. Chalcones are synthetic and naturally occurring compounds that have been widely investigated as anticancer agents. In this work, three chalcone derivatives were tested regarding their inhibitory activity and selectivity towards GBM cell lines (human and mouse) and a non-cancerous mouse brain cell line. The chalcone 1 showed the most potent and selective cytotoxic effects in the GBM cell lines, being further investigated regarding its ability to reduce critical hallmark features of GBM and to induce apoptosis and cell cycle arrest. This derivative showed to successfully reduce the invasion and proliferation capacity of tumor cells, both key targets for cancer treatment. Moreover, to overcome potential systemic side effects and its poor water solubility, this compound was encapsulated into liposomes. Therapeutic concentrations were incorporated retaining the potent in vitro growth inhibitory effect of the selected compound. In conclusion, our results demonstrated that this new formulation can be a promising starting point for the discovery of new and more effective drug treatments for GBM.
What about Dinner? Chemical and Microresidue Analysis Reveals the Function of Late Neolithic Ceramic Pans
Authors: Beneš Jaromír , Todoroska Valentina , Budilová Kristýna , Kovárník Jaromír , Pavelka Jaroslav , Atanasoska Nevenka , Bumerl Jiří , Florenzano Assunta , Majerovičová Tereza , Vondrovský Václav , Ptáková Michaela , Bednář Petr , Richtera Lukáš , Kučera Lukáš
Published: June 2021

The Late Neolithic palafitte site, Ustie na Drim, in the northern part of Lake Ohrid (North Macedonia), excavated in 1962, offered ceramic fragments of large, flat, elongated pans. These artifacts could be dated by relative chronology to roughly around 5200–5000 BC. According to their shape and technological traits, the ceramic pans were probably used for baking. The attached materials on the surface of studied pan fragments were sampled for consequent chemical and microscopical analyses (i.e., analyses of starch, phytoliths, and microscopic animal remains). An immunological method revealed the presence of pork proteins in samples. The presence of organic residues of animal origin was, moreover, confirmed by the detection of cholesterol using gas chromatography coupled to mass spectrometry. Analysis of detected microscopic botanical objects revealed starch grains of several plants (i.e., oak, cattail, and grasses). An interesting find was the hair of a beetle larva, which could be interpreted contextually as the khapra beetle, a pest of grain and flour. Based on our data, we suppose that the ceramic pans from Ustie na Drim were used for the preparation of meals containing meat from common livestock in combination with cereals and wild plants.
PLS-DA Model for the Evaluation of Attention Deficit and Hyperactivity Disorder in Children and Adolescents through Blood Serum FTIR Spectra
Authors: Ogruc Ildiz Gulce , Karadag Ahmet , Kaygisiz Ersin , Fausto Rui
Published: June 2021

Attention deficit and hyperactivity disorder (ADHD) is one of the most common neurodevelopmental disorders of childhood. It affects ~10% of the world’s population of children, and about 30–50% of those diagnosed in childhood continue to show ADHD symptoms later, with 2–5% of adults having the condition. Current diagnosis of ADHD is based on the clinical evaluation of the patient, and on interviews performed by clinicians with parents and teachers of the children, which, together with the fact that it shares common symptoms and frequent comorbidities with other neurodevelopmental disorders, makes the accurate and timely diagnosis of the disorder a difficult task. Despite the large effort to identify reliable biomarkers that can be used in a clinical environment to support clinical diagnosis, this goal has never been achieved hitherto. In the present study, infrared spectroscopy was used together with multivariate statistical methods (hierarchical clustering and partial least-squares discriminant analysis) to develop a model based on the spectra of blood serum samples that is able to distinguish ADHD patients from healthy individuals. The developed model used an approach where the whole infrared spectrum (in the 3700–900 cm−1 range) was taken as a holistic imprint of the biochemical blood serum environment (spectroscopic biomarker), overcoming the need for the search of any particular chemical substance associated with the disorder (molecular biomarker). The developed model is based on a sensitive and reliable technique, which is cheap and fast, thus appearing promising to use as a complementary diagnostic tool in the clinical environment.
Decaffeination and Neuraminidase Inhibitory Activity of Arabica Green Coffee (Coffea arabica) Beans: Chlorogenic Acid as a Potential Bioactive Compound
Authors: Muchtaridi Muchtaridi , Lestari Dwintha , Khairul Ikram Nur Kusaira , Gazzali Amirah Mohd , Hariono Maywan , Wahab Habibah A.
Published: June 2021

Coffee has been studied for its health benefits, including prevention of several chronic diseases, such as type 2 diabetes mellitus, cancer, Parkinson’s, and liver diseases. Chlorogenic acid (CGA), an important component in coffee beans, was shown to possess antiviral activity against viruses. However, the presence of caffeine in coffee beans may also cause insomnia and stomach irritation, and increase heart rate and respiration rate. These unwanted effects may be reduced by decaffeination of green bean Arabica coffee (GBAC) by treatment with dichloromethane, followed by solid-phase extraction using methanol. In this study, the caffeine and chlorogenic acid (CGA) level in the coffee bean from three different areas in West Java, before and after decaffeination, was determined and validated using HPLC. The results showed that the levels of caffeine were reduced significantly, with an order as follows: Tasikmalaya (2.28% to 0.097% (97 ppm), Pangalengan (1.57% to 0.049% (495 ppm), and Garut (1.45% to 0.00002% (0.2 ppm). The CGA levels in the GBAC were also reduced as follows: Tasikmalaya (0.54% to 0.001% (118 ppm), Pangalengan (0.97% to 0.0047% (388 ppm)), and Garut (0.81% to 0.029% (282 ppm). The decaffeinated samples were then subjected to the H5N1 neuraminidase (NA) binding assay to determine its bioactivity as an anti-influenza agent. The results show that samples from Tasikmalaya, Pangalengan, and Garut possess NA inhibitory activity with IC50 of 69.70, 75.23, and 55.74 μg/mL, respectively. The low level of caffeine with a higher level of CGA correlates with their higher levels of NA inhibitory, as shown in the Garut samples. Therefore, the level of caffeine and CGA influenced the level of NA inhibitory activity. This is supported by the validation of CGA-NA binding interaction via molecular docking and pharmacophore modeling; hence, CGA could potentially serve as a bioactive compound for neuraminidase activity in GBAC.
P∩N Bridged Cu(I) Dimers Featuring Both TADF and Phosphorescence. From Overview towards Detailed Case Study of the Excited Singlet and Triplet States
Authors: Hofbeck Thomas , Niehaus Thomas A. , Fleck Michel , Monkowius Uwe , Yersin Hartmut
Published: June 2021

We present an overview over eight brightly luminescent Cu(I) dimers of the type Cu2X2(P∩N)3 with X = Cl, Br, I and P∩N = 2-diphenylphosphino-pyridine (Ph2Ppy), 2-diphenylphosphino-pyrimidine (Ph2Ppym), 1-diphenylphosphino-isoquinoline (Ph2Piqn) including three new crystal structures (Cu2Br2(Ph2Ppy)3 1-Br, Cu2I2(Ph2Ppym)3 2-I and Cu2I2(Ph2Piqn)3 3-I). However, we mainly focus on their photo-luminescence properties. All compounds exhibit combined thermally activated delayed fluorescence (TADF) and phosphorescence at ambient temperature. Emission color, decay time and quantum yield vary over large ranges. For deeper characterization, we select Cu2I2(Ph2Ppy)3, 1-I, showing a quantum yield of 81%. DFT and SOC-TDDFT calculations provide insight into the electronic structures of the singlet S1 and triplet T1 states. Both stem from metal+iodide-to-ligand charge transfer transitions. Evaluation of the emission decay dynamics, measured from 1.2 ≤ T ≤ 300 K, gives ∆E(S1-T1) = 380 cm−1 (47 meV), a transition rate of k(S1→S0) = 2.25 × 106 s−1 (445 ns), T1 zero-field splittings, transition rates from the triplet substates and spin-lattice relaxation times. We also discuss the interplay of S1-TADF and T1-phosphorescence. The combined emission paths shorten the overall decay time. For OLED applications, utilization of both singlet and triplet harvesting can be highly favorable for improvement of the device performance.
Exosomes as Naturally Occurring Vehicles for Delivery of Biopharmaceuticals: Insights from Drug Delivery to Clinical Perspectives
Authors: Butreddy Arun , Kommineni Nagavendra , Dudhipala Narendar
Published: June 2021

Exosomes as nanosized vesicles are emerging as drug delivery systems for therapeutics owing to their natural origin, their ability to mediate intercellular communication, and their potential to encapsulate various biological molecules such as proteins and nucleic acids within the lipid bilayer membrane or in the lumen. Exosomes contain endogenous components (proteins, lipids, RNA) that could be used to deliver cargoes to target cells, offering an opportunity to diagnose and treat various diseases. Owing to their ability to travel safely in extracellular fluid and to transport cargoes to target cells with high efficacy, exosomes offer enhanced delivery of cargoes in vivo. However, several challenges related to the stabilization of the exosomes, the production of sufficient amounts of exosomes with safety and efficacy, the efficient loading of drugs into exosomes, the clearance of exosomes from circulation, and the transition from the bench scale to clinical production may limit their development and clinical use. For the clinical use of exosomes, it is important to understand the molecular mechanisms behind the transport and function of exosome vesicles. This review exploits techniques related to the isolation and characterization of exosomes and their drug delivery potential to enhance the therapeutic outcome and stabilization methods. Further, routes of administration, clinical trials, and regulatory aspects of exosomes will be discussed in this review.
Diagnostic Dilemma: An Atypical Case of Astrocytoma in a Patient with Relapsing–Remitting Multiple Sclerosis
Authors: Kahovec Chantal , Saini Aman , Levin Michael C.
Published: June 2021

Distinguishing between tumefactive demyelinating lesions (TDLs) and brain tumors in multiple sclerosis (MS) can be challenging. A progressive course is highly common with brain tumors in MS and no single neuroimaging technique is foolproof when distinguishing between the two. We report a case of a 41-year-old female with relapsing–remitting multiple sclerosis, who had a suspicious lesion within the left frontal hemisphere, without a progressive course. The patient experienced paresthesias primarily to her right hand but remained stable without any functional decline and new neurological symptoms over the four years she was followed. The lesion was followed with brain magnetic resonance imaging (MRI) scans, positron emission tomography–computed tomography scans, and magnetic resonance spectroscopy. Together, these scans favored the diagnosis of a TDL, but a low-grade tumor was difficult to rule out. Examination of serial brain MRI scans showed an enlarging lesion in the left middle frontal gyrus involving the deep white matter. Neurosurgery was consulted and an elective left frontal awake craniotomy was performed. Histopathology revealed a grade II astrocytoma. This case emphasizes the importance of thorough and continuous evaluation of atypical MRI lesions in MS and contributes important features to the literature for timely diagnosis and treatment of similar cases.
Nutrition and Kidney Stone Disease
Authors: Siener Roswitha
Published: June 2021

The prevalence of kidney stone disease is increasing worldwide. The recurrence rate of urinary stones is estimated to be up to 50%. Nephrolithiasis is associated with increased risk of chronic and end stage kidney disease. Diet composition is considered to play a crucial role in urinary stone formation. There is strong evidence that an inadequate fluid intake is the major dietary risk factor for urolithiasis. While the benefit of high fluid intake has been confirmed, the effect of different beverages, such as tap water, mineral water, fruit juices, soft drinks, tea and coffee, are debated. Other nutritional factors, including dietary protein, carbohydrates, oxalate, calcium and sodium chloride can also modulate the urinary risk profile and contribute to the risk of kidney stone formation. The assessment of nutritional risk factors is an essential component in the specific dietary therapy of kidney stone patients. An appropriate dietary intervention can contribute to the effective prevention of recurrent stones and reduce the burden of invasive surgical procedures for the treatment of urinary stone disease. This narrative review has intended to provide a comprehensive and updated overview on the role of nutrition and diet in kidney stone disease.