bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparison of Dobutamine, Norepinephrine, Vasopressin, and Hetastarch for the Treatment of Isoflurane-Induced Hypotension in Healthy, Normovolemic Dogs
Authors: Henao-Guerrero Natalia , Ricco-Pereira Carolina H. , Paranjape Vaidehi V.
Published: August 2023

Isoflurane is a commonly used inhalation anesthetic in species undergoing veterinary care that induces hypotension, impacting organ perfusion, making it imperative to minimize its occurrence or identify effective strategies for treating it. This study evaluated and compared the hemodynamic effects of DOB, NEP, VAS, and HES in twelve isoflurane-anesthetized Beagle dogs. The order of the first three treatments was randomized. HES was administered last. Data were collected before treatments (baseline) and after 10 min of a sustained MAP of <45 mmHg induced by a high end-tidal isoflurane concentration (T0). Once treatment was initiated and the target MAP was achieved (65 to 80 mmHg) or the maximum dose reached, data were collected after 15 min of stabilization (T1) and 15 min after (T2). A 15 min washout period with a MAP of ≥65 mmHg was allowed between treatments. The intravenous dosage regimens started and were increased by 50% every five minutes until the target MAP or maximum dose was reached. The dosages were as follows: DOB, 5–15 μg/kg/min; NEP, 0.1–2 μg/kg/min; VAS, 0.5–5 mU/kg/min; and HET, 6% 1–20 mL/kg/min. DOB improved CO, DO2, and VO2, but reduced SVR. VAS elevated SVR, but decreased CO, DO2, and VO2. HES minimally changed BP and mildly augmented CO, DO2, and VO2. These treatments failed to reach the target MAP. NEP increased the arterial BP, CO, MPAP, and PAWP, but reduced HR. Norepinephrine infusion at 0.44 ± 0.19 μg/kg/min was the most efficient therapy for correcting isoflurane-induced hypotension.
Elevated Interleukin-31 Levels in Serum, but Not CSF of Dogs with Steroid-Responsive Meningitis-Arteritis Suggest an Involvement in Its Pathogenesis
Authors: Lemke Laura , Carlson Regina , Warzecha Katrin , Volk Andrea V. , Tipold Andrea , Nessler Jasmin
Published: August 2023

Steroid-responsive meningitis-arteritis (SRMA) is a predominantly Th-2 immune-mediated disease, but the exact pathomechanism remains unclear. Interleukin-31 (IL-31) is predominantly produced by T cells with a Th-2 phenotype during proinflammatory conditions. We hypothesize that IL-31 might be involved in the pathogenesis of SRMA. IL-31 was measured in archived samples (49 serum and 52 CSF samples) of dogs with SRMA, meningoencephalitis of unknown origin (MUO), infectious meningoencephalitis, and atopic dermatitis, and of healthy control dogs using a competitive canine IL-31 ELISA. The mean serum IL-31 level in dogs with SRMA (n = 18) was mildly higher compared to dogs with atopic dermatitis (n = 3, p = 0.8135) and MUO (n = 15, p = 0.7618) and markedly higher than in healthy controls (n = 10, p = 0.1327) and dogs with infectious meningoencephalitis (n = 3, no statistics). Dogs with SRMA in the acute stage of the disease and without any pre-treatment had the highest IL-31 levels. The mean CSF IL-31 value for dogs with SRMA (n = 23) was quite similar to that for healthy controls (n = 8, p = 0.4454) and did not differ markedly from dogs with MUO (n = 19, p = 0.8724) and infectious meningoencephalitis. Based on this study, an involvement of IL-31 in the pathogenesis of the systemic Th-2 immune-mediated immune response in SRMA can be assumed as a further component leading to an aberrant immune reaction.
Predictive Factors for Two-Year Survival in Dogs with Hepatobiliary Diseases: Importance of Clinical and Laboratory Monitoring
Authors: Assawarachan Sathidpak Nantasanti , Ongvisespaibool Thodsapol , Hakhen Benjang , Chuchalermporn Piyathip , Maneesaay Phudit , Thengchaisri Naris
Published: August 2023

Long-term outcomes and survival predictors for different clinicopathologies (idiopathic chronic hepatitis, liver fibrosis, vacuolar hepatopathy) in dogs with hepatobiliary diseases are poorly described. In this study, ninety dogs were followed up for up to five years to investigate clinical factors that predict two-year survival in canine patients after liver biopsy. Univariate and multivariate analyses were performed based on clinical and laboratory data to determine the association between clinical and laboratory data and mortality rates. Overall, the one-, two-, and five-year mortality rates were 28.9%, 45.6%, and 78.9%, respectively. Univariate analysis indicated that male gender, ascites, elevated serum gamma-glutamyl transpeptidase (GGT), hypercholesterolemia, hypoalbuminemia, prolonged activated partial thromboplastin clotting time (aPTT), and prolonged thrombin clotting time (TT) were associated with an increased two-year mortality rate. Results from multivariate analysis demonstrated a significant association between male gender (p = 0.022), elevated serum GGT (p < 0.001), hypoalbuminemia (p < 0.001), and prolonged aPTT (p < 0.001) and an increased two-year mortality rate, regardless of the specific type of liver pathology. Elevated GGT was associated with the highest risk for increased two-year mortality (95% CI: hazard ratio 6.02–41.21). In conclusion, various clinical factors in dogs with liver diseases are useful for prognosis prediction.
Home Sweet Home: The Impact of Lifestyle on a Cat’s Approach to Impossible Tasks in the Home Environment
Authors: Scandurra Anna , Di Lucrezia Alfredo , D’Aniello Biagio , Pinelli Claudia
Published: August 2023

Cat welfare is a topic of growing interest in the scientific literature. Although previous studies have focused on the effects of living style (i.e., indoor/outdoor) on cat welfare, there has been a noticeable dearth of analysis regarding the impact of lifestyle on cats’ inclination and mode of communication with humans. Our research aimed to analyze the possible effect of lifestyle (e.g., living indoors only or indoor/outdoor) on cat–human communication. The cats were tested using the impossible task paradigm test, which consists of some solvable trials in which the subject learns to obtain a reward from an apparatus, followed by an impossible trial through blocking the apparatus. This procedure triggers a violation of expectations and is considered a useful tool for assessing both the decision-making process and the tendency to engage in social behaviors towards humans. A specific ethogram was followed to record the behavioral responses of the cats during the unsolvable trial. Our results show the effects of lifestyle and age on domestic cats, providing valuable insights into the factors that influence their social behaviors. Cats that can roam freely outdoors spent less time interacting with the apparatus compared to indoor-only cats. Additionally, roaming cats showed stress behaviors sooner following the expectancy of violation compared to indoor cats. The lifestyle of cats can influence their problem-solving approach while not affecting their willingness to interact with humans or their overall welfare. Future studies on this topic can be useful for improving the welfare of domestic cats.
Using Recombinant Superoxide Dismutase to Control Oxidative Stress in the Gastrointestinal Tract of Cyclic Heat-Stressed Pigs
Authors: Le Hieu Huu , Zhao Weicheng , Furness John Barton , Shakeri Majid , DiGiacomo Kristy , Roura Eugeni , Renaudeau David , Gabler Nicolas Kurt , Leury Brian Joseph , Dunshea Frank Rowland , Wijffels Gene , Cottrell Jeremy James
Published: August 2023

Climate change is associated with an increased frequency and intensity of heat waves, posing a threat of heat stress to pig production. Heat stress compromises the efficiency of pig production partly due to causing oxidative stress, intestinal dysfunction, and inflammatory responses. Superoxide dismutase is an antioxidant enzyme reported to reduce oxidative stress and inflammation. Therefore, this experiment aimed to investigate whether recombinant superoxide dismutase (rSOD) could ameliorate oxidative stress and inflammatory responses in heat-stressed grower pigs. Sixty-four female pigs (Large White × Landrace, 27.8 ± 1.65 kg, mean ± SD) were randomly allocated to a control diet (standard grower feed, CON) or the control diet supplemented with 50 IU recombinant superoxide dismutase (rSOD) for 14 days. After acclimation to the diet, pigs were then housed under thermoneutral (TN, 20 °C, 35–50% relative humidity) or cyclic heat stress conditions (CHS, at 35 °C: 9 a.m. to 5 p.m. and 28 °C: 5 p.m. to 9 a.m., 35–50% relative humidity) for 3 days. Heat stress increased respiration rate (RR), skin and rectal temperature (RR and RT) (p < 0.001 for all), and reduced plasma thyroid hormone concentration (p < 0.001). The amount of oxidized glutathione (GSH:GSSG) was increased in the jejunum and ileum of CHS pigs. In the jejunum, rSOD also increased the amount of oxidized glutathione in both TN and CHS pigs, without any change in endogenous SOD activity. In the ileum, rSOD prevented increases in oxidized glutathione formation in the CHS pigs only. Taken together, this may reflect increased oxidative stress in both the jejunum and ileum in CHS pigs. Alternatively, rSOD increased the conversion of reduced to oxidized glutathione independently of CHS, possibly reflecting an increased overall SOD activity due to the addition of exogenous SOD. In conclusion, the use of in-feed SOD enzymes at a dose of 50 IU/kg may be a useful strategy for preventing oxidative stress in pigs.
Effects and Mechanisms of Cage versus Floor Rearing System on Goose Growth Performance and Immune Status
Authors: Tang Bincheng , Hu Shenqiang , Zhang Xin , Ouyang Qingyuan , Qing Enhua , Wang Wanxia , Hu Jiwei , Li Liang , Wang Jiwen
Published: August 2023

Currently, FRS and CRS are the two predominant dryland rearing systems in the goose industry. However, the effects of these two systems on goose growth performance and health, as well as the underlying mechanisms, have not been fully clarified. Thus, this study aimed to compare growth performance and immune status, as well as investigate the genome-wide transcriptomic profiles of spleen in geese, between CRS and FRS at 270 d of age. Phenotypically, the body weight and body size traits were higher in geese under FRS, while the weight and organ index of spleen were higher in geese under CRS (p < 0.05). Noticeably, the bursa of Fabricius of geese under FRS was degenerated, while that under CRS was retained. At the serum level, the immune globulin-G (IgG) and interleukin-6 (IL-6) levels were higher in geese under CRS (p < 0.05). At the transcriptomic level, we identified 251 differentially expressed genes (DEGs) in the spleen between CRS and FRS, which were mainly enriched in scavenger receptor activity, inflammatory response, immune response, neuroactive ligand–receptor interaction, phenylalanine metabolism, ECM receptor interaction, calcium signaling pathway, phenylalanine, tyrosine, and tryptophan biosynthesis, regulation of actin cytoskeleton, and MAPK signaling pathways. Furthermore, through protein–protein interaction (PPI) network analysis, ten candidate genes were identified, namely, VEGFA, FGF2, NGF, GPC1, NKX2-5, FGFR1, FGF1, MEIS1, CD36, and PAH. Further analysis demonstrated that geese in CRS could improve their immune ability through the “phenylalanine metabolism” pathway. Our results revealed that the FRS improved growth performance, whereas the CRS improved goose immune function by increasing levels of IL-6 and IgG in serum. Moreover, the phenylalanine metabolism pathway could exert positive effects on immune function of geese under CRS. These results can provide reliable references for understanding how floor and cage rearing systems affect goose growth performance and immune capacity.
Browsers or Grazers? New Insights into Feral Burro Diet Using a Non-Invasive Sampling and Plant DNA Metabarcoding Approach
Authors: Esmaeili Saeideh , King Sarah R. B. , Schoenecker Kathryn A.
Published: August 2023

Ungulates play a large role in shaping ecosystems and communities by influencing plant composition, structure, and productivity. We investigated the summer diets of feral burros in two ecosystems in which they are found in the United States: a subtropical desert in Arizona and a temperate juniper shrubland in Utah. Between 24 June and 16 July of 2019, we gathered 50 burro fecal samples from each location and used plant DNA metabarcoding to determine the burros’ diets. We found that during our sampling period the burros in the Sonoran Desert consumed a higher proportion of woody browse and had a narrower dietary niche breadth and lower degree of diet diversity compared to the burros in the juniper shrubland ecosystem, where the burros consumed higher proportions of graminoids and forbs and had a higher diet diversity index and broader dietary niche breadth. The burros in the Sonoran Desert relied primarily on Prosopis spp. (mesquite) and Poaceae grasses, whereas the burros in the juniper shrubland relied on a wider variety of forb and grass species, likely due to the greater variability in the forage species temporally and spatially available in that temperate ecosystem. We found that feral burros are highly adaptable with respect to diet and appear to be employing a mixed feeding strategy, similar to their ancestor, the African wild ass, to meet their nutritional needs in whichever ecosystem they are found.
Correction: Messele et al. Longitudinal Analysis of Antimicrobial Resistance among Enterococcus Species Isolated from Australian Beef Cattle Faeces at Feedlot Entry and Exit. Animals 2022, 12, 2690
Authors: Messele Yohannes E. , Hasoon Mauida F. , Trott Darren J. , Veltman Tania , McMeniman Joe P. , Kidd Stephen P. , Low Wai Y. , Petrovski Kiro R.
Published: August 2023

There was an error in the original publication [...]
Experimental Investigation of the Desalination Process for Direct Contact Membrane Distillation Using Plate and Frame Membrane Module
Authors: Zhou Yukang , Chen Long , Huang Mengtao , Hu Weilian , Chen Guicai , Wu Binxin
Published: August 2023

Through experiments, the effect of membrane material selection and operating conditions on permeate fluxes in direct contact membrane distillation (DCMD) desalination was investigated. The experiment used a plate and frame membrane module, and with nine different hydrophobic porous membranes, a comparative analysis of the desalination performance of 3 wt% NaCl solution was performed. The results of this experiment were compared to find out the effect of different materials, pore sizes and membrane thicknesses on the permeate flux under same operating conditions. Further, a three-factor, three-level orthogonal experiment was designed. The effects of hot-side temperature, hot-side inlet flow and cold-side inlet flow on the permeate flux of PTFE membranes with a pore size of 0.22 μm were investigated when the temperature on the cold side was set at 20 °C. The results showed that in the DCMD experiments, both PTFE and PVDF membranes performed well, and that hot-side inlet temperatures and cold-side inlet flow rates had significant effects on the permeate flux.
Characteristics of Advection Fog at Qingdao Liuting International Airport
Authors: Zhang Zhiwei , Li Yunying , Li Laurent , Zhang Chao , Sun Guorong
Published: August 2023

The advection fog characteristics at Qingdao Liuting International Airport during 2000–2022 are studied based on surface observation, sounding and reanalysis data. Surface observation data show that there were two types of fog: evaporation fog (EF) dominated by northwesterly wind in winter and cooling fog (CF) dominated by southeasterly wind in spring and summer. CF is thicker than EF due to different planetary boundary layer (PBL) structures. For EF, the middle and low troposphere are affected by dry and cold air, while CF is affected by warm and moist air below 850 hPa. When EF formed, downdrafts and a positive vertical gradient of the pseudo-equivalent potential temperature indicate stable PBL, surface heat flux is upward from sea to atmosphere and surface wind diverges near the air–sea interface. When CF formed, these characteristics are reversed. Fog is significantly affected by sea–land–atmosphere interactions. The moisture source is mainly from surface fluxes released by the Yellow Sea in the case of EF, while it is from moist air at low latitudes and local land transpiration in the case of CF. The difference in temperature between the sea surface and surface air changes from the range of 0–8 K for EF but from −4–0 K for CF.
Advances of Phase-Field Model in the Numerical Simulation of Multiphase Flows: A Review
Authors: Li Jingfa , Zheng Dukui , Zhang Wei
Published: August 2023

The phase-field model (PFM) is gaining increasing attention in the application of multiphase flows due to its advantages, in which the phase interface is treated as a narrow layer and phase parameters change smoothly and continually at this thin layer. Thus, the construction or tracking of the phase interface can be avoided, and the bulk phase and phase interface can be simulated integrally. PFM provides a useful alternative that does not suffer from problems with either the mass conservation or the accurate computation of surface tension. In this paper, the state of the art of PFM in the numerical modeling and simulation of multiphase flows is comprehensively reviewed. Starting with a brief description of historical developments in the PFM, we continue to take a tour into the basic concepts, fundamental theory, and mathematical models. Then, the commonly used numerical schemes and algorithms for solving the governing systems of PFM in the application of multiphase flows are presented. The various applications and representative results, especially in non-match density scenarios of multiphase flows, are reviewed. The primary challenges and research focus of PFM are analyzed and summarized as well. This review is expected to provide a valuable reference for PFM in the application of multiphase flows.
Shipping Emissions and Air Pollution: Latest Methodological Developments and Applications
Authors: Zhu Yuanqing , Liu Long
Published: August 2023

Shipping, which accounts for over 80% of international trade transportation, is the most cost-effective and efficient mode of transportation [...]
Exact Intermittent Solutions in a Turbulence Multi-Branch Shell Model
Authors: Ajzner Ben , Alexakis Alexandros
Published: August 2023

Reproducing complex phenomena with simple models marks our understanding of the phenomena themselves, and this is what Jack Herring’s work demonstrated multiple times. In that spirit, this work studies a turbulence shell model consisting of a hierarchy of structures of different scales ℓn such that each structure transfers its energy to two substructures of scale ℓn+1=ℓn/λ. For this model, we construct exact inertial range solutions that display intermittency, i.e., absence of self-similarity. Using a large ensemble of these solutions, we investigate how the probability distributions of the velocity modes change with scale. It is demonstrated that, while velocity amplitudes are not scale-invariant, their ratios are. Furthermore, using large deviation theory, we show how the probability distributions of the velocity modes can be re-scaled to collapse in a scale-independent form. Finally, we discuss the implications the present results have for real turbulent flows.
Thermospheric Density Response to the QBO Signal
Authors: Li Bo , Cui Ruifei , Weng Libin
Published: August 2023

In this study, we focused on the periodic variations of global average thermospheric density, derived from orbital decay measurements of about 5000 space objects from 1967 to 2013, by using the wavelet power spectrum method. The results demonstrated that the thermospheric density showed an ~11-year period, with semiannual and annual variations, while the seasonal variation was usually more significant under high solar activity conditions. Importantly, we investigated the possible link between the thermospheric density and the QBO, with the aid of the Global Average Mass Density Model (GAMDM) and the different density residuals method. The difference between the measured density and the GAMDM empirical model seemingly had QBO signal, but the ratio of them revealed that the QBO signal could not detect in the thermospheric density. Comprehensively, we found that the stratospheric QBO cannot impact on the thermosphere, and more data and numerical modeling are needed for further validation.
Kalman Filter and Its Application in Data Assimilation
Authors: Wang Bowen , Sun Zhibin , Jiang Xinyue , Zeng Jun , Liu Runqing
Published: August 2023

In 1960, R.E. Kalman published his famous paper describing a recursive solution, the Kalman filter, to the discrete-data linear filtering problem. In the following decades, thanks to the continuous progress of numerical computing, as well as the increasing demand for weather prediction, target tracking, and many other problems, the Kalman filter has gradually become one of the most important tools in science and engineering. With the continuous improvement of its theory, the Kalman filter and its derivative algorithms have become one of the core algorithms in optimal estimation. This paper attempts to systematically collect and sort out the basic principles of the Kalman filter and some of its important derivative algorithms (mainly including the Extended Kalman filter (EKF), the Unscented Kalman filter (UKF), the Ensemble Kalman filter (EnKF)), as well as the scope of their application, and also to compare their advantages and limitations. In addition, because there are a large number of applications based on the Kalman filter in data assimilation, this paper also provides examples and classifies the applications of both the Kalman filter and its derivative algorithms in the field of data assimilation.
Functional Changes of White Matter Are Related to Human Pain Sensitivity during Sustained Nociception
Authors: He Hui , Hu Lan , Tan Saiying , Tang Yingjie , Duan Mingjun , Yao Dezhong , Zhao Guocheng , Luo Cheng
Published: August 2023

Pain is considered an unpleasant perceptual experience associated with actual or potential somatic and visceral harm. Human subjects have different sensitivity to painful stimulation, which may be related to different painful response pattern. Excellent studies using functional magnetic resonance imaging (fMRI) have found the effect of the functional organization of white matter (WM) on the descending pain modulatory system, which suggests that WM function is feasible during pain modulation. In this study, 26 pain sensitive (PS) subjects and 27 pain insensitive (PIS) subjects were recruited based on cold pressor test. Then, all subjects underwent the cold bottle test (CBT) in normal (26 degrees temperature stimulating) and cold (8 degrees temperature stimulating) conditions during fMRI scan, respectively. WM functional networks were obtained using K-means clustering, and the functional connectivity (FC) was assessed among WM networks, as well as gray matter (GM)–WM networks. Through repeated measures ANOVA, decreased FC was observed between the GM–cerebellum network and the WM–superior temporal network, as well as the WM–sensorimotor network in the PS group under the cold condition, while this difference was not found in PIS group. Importantly, the changed FC was positively correlated with the state and trait anxiety scores, respectively. This study highlighted that the WM functional network might play an integral part in pain processing, and an altered FC may be related to the descending pain modulatory system.
Effect of Mesenchymal Stem Cells Overexpressing BMP-9 Primed with Hypoxia on BMP Targets, Osteoblast Differentiation and Bone Repair
Authors: Paz Jessica Emanuella Rocha Moura , Adolpho Leticia Faustino , Ramos Jaqueline Isadora Reis , Bighetti-Trevisan Rayana Longo , Calixto Robson Diego , Oliveira Fabiola Singaretti , Almeida Adriana Luisa Gonçalves , Beloti Marcio Mateus , Rosa Adalberto Luiz
Published: August 2023

Bone formation is driven by many signaling molecules including bone morphogenetic protein 9 (BMP-9) and hypoxia-inducible factor 1-alpha (HIF-1α). We demonstrated that cell therapy using mesenchymal stem cells (MSCs) overexpressing BMP-9 (MSCs+BMP-9) enhances bone formation in calvarial defects. Here, the effect of hypoxia on BMP components and targets of MSCs+BMP-9 and of these hypoxia-primed cells on osteoblast differentiation and bone repair was evaluated. Hypoxia was induced with cobalt chloride (CoCl2) in MSCs+BMP-9, and the expression of BMP components and targets was evaluated. The paracrine effects of hypoxia-primed MSCs+BMP-9 on cell viability and migration and osteoblast differentiation were evaluated using conditioned medium. The bone formation induced by hypoxia-primed MSCs+BMP-9 directly injected into rat calvarial defects was also evaluated. The results demonstrated that hypoxia regulated BMP components and targets without affecting BMP-9 amount and that the conditioned medium generated under hypoxia favored cell migration and osteoblast differentiation. Hypoxia-primed MSCs+BMP-9 did not increase bone repair compared with control MSCs+BMP-9. Thus, despite the lack of effect of hypoxia on bone formation, the enhancement of cell migration and osteoblast differentiation opens windows for further investigations on approaches to modulate the BMP-9-HIF-1α circuit in the context of cell-based therapies to induce bone regeneration.
In Silico Simulations Reveal Molecular Mechanism of Uranyl Ion Toxicity towards DNA-Binding Domain of PARP-1 Protein
Authors: Bulavko Egor S. , Pak Marina A. , Ivankov Dmitry N.
Published: August 2023

The molecular toxicity of the uranyl ion (UO22+) in living cells is primarily determined by its high affinity to both native and potential metal-binding sites that commonly occur in the structure of biomolecules. Recent advances in computational and experimental research have shed light on the structural properties and functional impacts of uranyl binding to proteins, organic ligands, nucleic acids, and their complexes. In the present work, we report the results of the computational investigation of the uranyl-mediated loss of DNA-binding activity of PARP-1, a eukaryotic enzyme that participates in DNA repair, cell differentiation, and the induction of inflammation. The latest experimental studies have shown that the uranyl ion directly interacts with its DNA-binding subdomains, zinc fingers Zn1 and Zn2, and alters their tertiary structure. Here, we propose an atomistic mechanism underlying this process and compute the free energy change along the suggested pathway. Our Quantum Mechanics/Molecular Mechanics (QM/MM) simulations of the Zn2-UO22+ complex indicate that the uranyl ion replaces zinc in its native binding site. However, the resulting state is destroyed due to the spontaneous internal hydrolysis of the U-Cys162 coordination bond. Despite the enthalpy of hydrolysis being +2.8 kcal/mol, the overall reaction free energy change is −0.6 kcal/mol, which is attributed to the loss of domain’s native tertiary structure originally maintained by a zinc ion. The subsequent reorganization of the binding site includes the association of the uranyl ion with the Glu190/Asp191 acidic cluster and significant perturbations in the domain’s tertiary structure driven by a further decrease in the free energy by 6.8 kcal/mol. The disruption of the DNA-binding interface revealed in our study is consistent with previous experimental findings and explains the loss of PARP-like zinc fingers’ affinity for nucleic acids.
Trigeminal Stimulation and Visuospatial Performance: The Struggle between Chewing and Trigeminal Asymmetries
Authors: Tramonti Fantozzi Maria Paola , De Cicco Vincenzo , d’Ascanio Paola , Cataldo Enrico , De Cicco Davide , Bruschini Luca , Barresi Massimo , Faraguna Ugo , Manzoni Diego
Published: August 2023

Chewing improves visuospatial performance through locus coeruleus (LC) activation. The effects of bilateral and unilateral mastication were investigated in subjects showing different degrees of asymmetry in masseter electromyographic (EMG) activity during clenching and in pupil size at rest (anisocoria), which is a proxy of LC imbalance. Correlations between performance changes and asymmetry values were found in males, but not in females. Among males, subjects with low asymmetry values (balanced-BAL) were more sensitive than those with high asymmetry values (imbalanced-IMB) to bilateral and unilateral chewing on the side with higher EMG activity (hypertonic). The opposite was true for hypotonic side chewing. BAL subjects were sensitive to unilateral chewing on both sides, while in IMB subjects, hypertonic side chewing did not influence performance in either males or females. Bilateral chewing elicited larger effects in BAL subjects than in IMB subjects, exceeding the values predicted from unilateral chewing in both groups. Finally, pupil size and anisocoria changes elicited by chewing were correlated with asymmetry values, independent of sex. Data confirmed the facilitation of visuospatial performance exerted by chewing. Trigeminal asymmetries modulate the chewing effects, making occlusal rebalancing an appropriate strategy to improve performance.
Catfish Egg Lectin Enhances the Cytotoxicity of Sunitinib on Gb3-Expressing Renal Cancer Cells
Authors: Ito Jun , Sugawara Shigeki , Tatsuta Takeo , Hosono Masahiro , Sato Makoto
Published: August 2023

Metastatic renal cell carcinoma (RCC) is not sufficiently responsive to anticancer drugs, and thus, developing new drugs for advanced RCC remains vital. We previously reported that the treatment of globotriaosylceramide (Gb3)-expressing cells with catfish (Silurus asotus) egg lectin (SAL) increased the intracellular uptake of propidium iodide (PI) and sunitinib (SU). Herein, we investigated whether SAL pretreatment affects the intracellular uptake and cytotoxic effects of molecular-targeted drugs in RCC cells. We analyzed Gb3 expression in TOS1, TOS3, TOS3LN, and ACHN human RCC cells. Surface Gb3 expression was higher in TOS1 and TOS3 cells than in TOS3LN and ACHN cells. In the PI uptake assay, 41.5% of TOS1 cells and 21.1% of TOS3 cells treated with SAL were positive for PI. TOS1 cell viability decreased to 70% after treatment with 25 µM SU alone and to 48% after pretreatment with SAL (50 µg/mL). Time-series measurements of the intracellular fluorescence of SU revealed significantly enhanced SU uptake in SAL-treated TOS1 cells compared to control cells. SAL treatment did not increase PI uptake in normal renal cells. Our findings suggest that adequate cytotoxic activity may be achieved even when SU is administered at a sufficiently low dose not to cause side effects in combination with SAL.
Sensory Modulation Abilities in Healthy Preterm-Born Children: An Observational Study Using the Sensory Processing and Self-Regulation Checklist (SPSRC)
Authors: Previtali Giulia , Lai Cynthia Y. Y. , Valvassori Bolgè Maria , Cavallini Anna , Nacinovich Renata , Piscitelli Daniele , Purpura Giulia
Published: August 2023

This study aimed to investigate prematurity as a risk factor for sensory processing disorders, using the Italian Version of Sensory Processing and Self-Regulation Checklist (SPSRC-IT), based on a sample of healthy Italian children born preterm in comparison with a sample of typical full-term children. Two groups of caregivers of Italian healthy preschooler children were recruited. The first group comprised 37 caregivers of full-term children (FT), while the second group consisted of 37 caregivers of preterm children (PT) (gestational age < 37 weeks). Significant differences between the groups in several subsections and factors of the SPSRC-IT were found, specifically in the Physiological Conditions section, in the Gustatory and Olfactory Sense section, in the Vestibular Sense section, and in the Proprioceptive Sense section, with lower scores in the PT group. Moreover, children born at a lower gestational age or with lower weights had a higher risk of dysfunctions in processing gustatory and olfactory, vestibular, and proprioceptive stimuli. In conclusion, the SPSRC-IT suggested a potential link between prematurity and challenges in the development of sensory processing and self-regulation skills, especially in children with a very low birth weight and very low gestational age.
Induction of Skin Cancer by Long-Term Blue Light Irradiation
Authors: Hiramoto Keiichi , Kubo Sayaka , Tsuji Keiko , Sugiyama Daijiro , Hamano Hideo
Published: August 2023

Presently, people are not only exposed to sunlight but also to a large amount of blue light from personal computers and smartphones. This blue light has various effects on the living body. However, its effect on the induction of skin cancer is unknown. In this study, we investigated the induction of skin cancer by long-term blue light irradiation. Hairless mice were irradiated with blue light (LED; peak emission 479 nm) every day for one year, and a control was irradiated with white light (LED), green light (LED; peak emission 538 nm), and red light (LED; peak emission 629 nm) for one year, respectively. Skin cancer was induced only in the mice exposed to blue light. Long-term blue light irradiation also increased the migration of neutrophils and macrophages involved in carcinogenesis in the skin. In neutrophils, an increased expression of citH3 and PAD4 was observed, suggesting the possibility of NETosis. Conversely, in macrophages, inflammatory macrophages (type 1 macrophages) increased and anti-inflammatory macrophages (type 2 macrophages) decreased due to continuous blue light irradiation. These findings suggest that long-term continuous irradiation with blue light induces neutrophil NETosis and an increase in type 1 macrophages, resulting in skin cancer.
Kinematic Behavior of an Untethered, Small-Scale Hydrogel-Based Soft Robot in Response to Magneto-Thermal Stimuli
Authors: Pan Wenlong , Gao Chongyi , Zhu Chen , Yang Yabing , Xu Lin
Published: August 2023

Fruit fly larvae, which exist widely in nature, achieve peristaltic motion via the contraction and elongation of their bodies and the asymmetric friction generated by the front and rear parts of their bodies when they are in contact with the ground. Herein, we report the development of an untethered, magnetic, temperature-sensitive hydrogel-based soft robot that mimics the asymmetric micro-patterns of fruit-fly-larvae gastropods and utilizes cyclic deformation to achieve directional peristaltic locomotion. Due to Néel relaxation losses of nanomagnetic Fe3O4 particles, the hydrogel-based soft robot is capable of converting changes in external alternating magnetic stimuli into contracting and expanding deformation responses which can be remotely controlled via a high-frequency alternating magnetic field (AMF) to realize periodic actuation. Furthermore, the Fe3O4 particles included in the hydrogel-based soft robot cause it to follow a gradient magnetic field in confined liquid environments and can be coupled with AMFs for the targeted release of water-soluble drugs or targeted magnetic hyperthermia therapy (MHT). We believe that such a controlled motion can enable highly targeted drug delivery, as well as vascular disease detection and thrombus removal tasks, without the use of invasive procedures.
Magnetic Molecularly Imprinted Chitosan Combined with a Paper-Based Analytical Device for the Smartphone Discrimination of Tryptophan Enantiomers
Authors: Karrat Abdelhafid , García-Guzmán Juan José , Palacios-Santander José María , Amine Aziz , Cubillana-Aguilera Laura
Published: August 2023

The separation of enantiomers plays a critical role in pharmaceutical development, ensuring therapeutic efficacy, safety, and patent protection. It enables the production of enantiopure drugs and enhances our understanding of the properties of chiral compounds. In this study, a straightforward and effective chiral detection strategy was developed for distinguishing between tryptophan (TRP) enantiomers. The approach involved the preparation of a magnetic molecularly imprinted chitosan (MMIC) for preparation of the sample, which was combined with a nitrocellulose membrane (a paper-based analytical device, PAD) integrated with D-TRP covalently grafted with polymethacrylic acid (PAD-PMA_D-TRP). Discriminating between the TRP enantiomers was achieved using AuNPs as a colorimetric probe. Indeed, the presence of D-TRP rapidly induced the aggregation of AuNPs due to its strong affinity to PAD-PMA_D-TRP, resulting in a noticeable change in the color of the AuNPs from red to purple. On the other hand, L-TRP did not induce any color changes. The chiral analysis could be easily performed with the naked eye and/or a smartphone. The developed method exhibited a detection limit of 3.3 µM, and it was successfully applied to detect TRP in serum samples, demonstrating good recovery rates. The proposed procedure is characterized by its simplicity, cost-effectiveness, rapidity, and ease of operation.
A Miniature Biomedical Sensor for Rapid Detection of Schistosoma japonicum Antibodies
Authors: Hu Shengjie , Jiang Xuecheng , Yang Liang , Tang Xue , Yang Guofeng , Hu Yuanyuan , Wang Jie , Lu Naiyan
Published: August 2023

Schistosomiasis, typically characterized by chronic infection in endemic regions, has the potential to affect liver tissue and pose a serious threat to human health. Detecting and screening for this disease early on is crucial for its prevention and control. However, existing methods encounter challenges such as low sensitivity, time-consuming processes, and complex sample handling. To address these challenges, we report a soluble egg antigen (SEA)-based functionalized gridless and meander-type AlGaN/GaN high electron mobility transistors (HEMT) sensor for the highly sensitive detection of antibodies to Schistosoma japonicum. Immobilization of the self-assembled membrane on the gate surface was verified using a semiconductor parameter analyzer, scanning electron microscope (SEM), and atomic force microscopy (AFM). The developed biosensor demonstrates remarkable performance in detecting anti-SEA, exhibiting a linear concentration range of 10 ng/mL to 100 μg/mL and a sensitivity of 0.058 mA/log (ng/mL). It also exhibits similar excellent performance in serum systems. With advantages such as rapid detection, high sensitivity, miniaturization, and label-free operation, this biosensor can fulfill the requirements for blood defense.
Coupling Capillary-Driven Microfluidics with Lateral Flow Immunoassay for Signal Enhancement
Authors: Azizian Pooya , Casals-Terré Jasmina , Guerrero-SanVicente Elena , Grinyte Ruta , Ricart Jordi , Cabot Joan M.
Published: August 2023

Microfluidics has emerged as a versatile technology that is applied to enhance the performance of analytical techniques, among others. Pursuing this, we present a capillary-driven microfluidic device that improves the sensitivity of lateral flow immunoassay rapid tests thanks to offering an automated washing step. A novel multilevel microfluidic chip was 3D-printed with a photocurable black resin, sealed by an optically clear pressure-sensitive adhesive, and linked to the lateral flow strip. To depict the efficacy of microfluidics and the washing step, cortisol was measured quantitatively within the proposed device. Measuring cortisol levels is a way to capture physiological stress responses. Among biofluids, saliva is less infectious and easier to sample than others. However, higher sensitivity is demanded because the salivary cortisol concentrations are much lower than in blood. We carried out a competitive lateral flow immunoassay protocol with the difference that the microfluidic device applies an automated washing step after the sample is drained downstream. It washes the trapped quantum-dot-labeled antibodies out from nitrocellulose, diminishing background noise as these are bonded to cortisols and not to the immobilized receptors. Fluorescence spectroscopy, as a high-precision analysis, was successfully applied to determine clinically relevant salivary cortisol concentrations within a buffer quantitatively. The microfluidic design relied on a 3D valve that avoids reagent cross-contamination. This cross-contamination could make the washing buffer impure and undesirably dilute the sample. The proposed device is cost-effective, self-powered, robust, and ideal for non-expert users.
Can Brain-Derived Neurotrophic Factor Be Considered a Biomarker for Bipolar Disorder? An Analysis of the Current Evidence
Authors: De Felice Gianmarco , Luciano Mario , Boiano Alessia , Colangelo Giulia , Catapano Pierluigi , Della Rocca Bianca , Lapadula Maria Vita , Piegari Elena , Toni Claudia , Fiorillo Andrea
Published: August 2023

Brain-derived neurotrophic factor (BDNF) plays a key role in brain development, contributing to neuronal survival and neuroplasticity. Previous works have found that BDNF is involved in several neurological or psychiatric diseases. In this review, we aimed to collect all available data on BDNF and bipolar disorder (BD) and assess if BDNF could be considered a biomarker for BD. We searched the most relevant medical databases and included studies reporting original data on BDNF circulating levels or Val66Met polymorphism. Only articles including a direct comparison with healthy controls (HC) and patients diagnosed with BD according to international classification systems were included. Of the 2430 identified articles, 29 were included in the present review. Results of the present review show a reduction in BDNF circulating levels during acute phases of BD compared to HC, which increase after effective therapy of the disorders. The Val66Met polymorphism was related to features usually associated with worse outcomes. High heterogeneity has been observed regarding sample size, clinical differences of included patients, and data analysis approaches, reducing comparisons among studies. Although more studies are needed, BDNF seems to be a promising biomarker for BD.
Elevated Plasma Levels of Mature Brain-Derived Neurotrophic Factor in Major Depressive Disorder Patients with Higher Suicidal Ideation
Authors: Li Haimei , Zhao Miaomiao , Jiang Chaonan , Zhao Haoyang , Wu Congchong , Li Ying , Zhang Shiyi , Xu Pengfeng , Mou Tingting , Xu Yi , Huang Manli
Published: August 2023

Several pieces of evidence show that signaling via brain-derived neurotrophic factor (BDNF) and its receptor, tropomycin receptor kinase B (TrkB), as well as inflammation, play a crucial part in the pathophysiology of depression. The purpose of our study was to evaluate plasma levels of BDNF-TrkB signaling, which are inflammatory factors in major depressive disorder (MDD) patients, and assess their associations with clinical performance. This study recruited a total sample of 83 MDD patients and 93 healthy controls (CON). All the participants were tested with the Hamilton Depression Scale (HAMD), the Beck Scale for Suicide Ideation, and the NEO Five-Factor Inventory. The plasma level of selected BDNF-TrkB signaling components (mature BDNF (mBDNF), precursor BDNF (proBDNF), tyrosine kinase B (TrkB), and tissue plasminogen activator (tPA)) and selected inflammatory factors (interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α)) were measured using an enzyme-linked immunosorbent assay (ELISA). Further, we performed correlation analysis to indicate the relationship between the plasma levels of the factors and clinical characteristics. Results: (i) A higher level of mBDNF and lower openness were observed in MDD patients with higher suicidal ideation than patients with lower suicidal ideation. (ii) In MDD patients, mBDNF was positively correlated with the sum score of the Beck Scale for Suicide Ideation (BSS). (iii) The levels of mBDNF, tPA, IL-1 β and IL-6 were significantly higher in all MDD subjects compared to the healthy controls, while the levels of TrkB and proBDNF were lower in MDD subjects. Conclusion: Our study provides novel insights regarding the potential role of mBDNF in the neurobiology of the association between depression and suicidal ideation and, in particular, the relationship between BDNF-TrkB signaling, inflammatory factors, and clinical characteristics in MDD.
Metabolomics Analysis of DRG and Serum in the CCI Model of Mice
Authors: Lu Kaimei , Fang Bin , Liu Yuqi , Xu Fangxia , Zhou Chengcheng , Wang Lijuan , Chen Lianhua , Huang Lina
Published: August 2023

Neuropathic pain (NP) is a chronic and intractable disease that is widely present in the general population. It causes painful behavior and even mood changes such as anxiety and depression by altering the metabolism of substances. However, there have been limited metabolomics studies conducted in relation to neuropathic pain. Therefore, in this study, the effects of NP on metabolites in serum and the dorsal root ganglion (DRG) were investigated using a non-targeted metabolomics approach detected by gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) to uncover differential metabolites and affected metabolic pathways associated with NP. Sixty mice were divided into the following two groups: a chronic constriction injury (CCI) of the sciatic nerve group and a sham group (n = 30, each). After 7 days of CCI modeling, the metabolite profiles of serum and the DRG were analyzed using GC/LC-MS for both the CCI and sham groups of mice. Multivariate analysis revealed differential metabolites and altered metabolic pathways between the CCI and sham groups. In the CCI group, our findings provided insights into the complex phospholipid, amino acid and acylcarnitine metabolic perturbations of DRG metabolism. In addition, phospholipid metabolic disorders and impaired glucose metabolism were observed in the serum. Moreover, the metabolic differences in the DRG and serum were correlated with each other. The results from this untargeted metabolomics study provide a perspective on the metabolic impact of NP on serum and the DRG.
The Influence of Transcranial Alternating Current Stimulation on Fatigue Resistance
Authors: De Guzman Kayla A. , Young Richard J. , Contini Valentino , Clinton Eliza , Hitchcock Ashley , Riley Zachary A. , Poston Brach
Published: August 2023

Previous research has shown that some forms of non-invasive brain stimulation can increase fatigue resistance. The purpose of this study is to determine the influence of transcranial alternating current stimulation (tACS) on the time to task failure (TTF) of a precision grip task. The study utilized a randomized, double-blind, SHAM-controlled, within-subjects design. Twenty-six young adults completed two experimental sessions (tACS and SHAM) with a 7-day washout period between sessions. Each session involved a fatiguing isometric contraction of the right hand with a precision grip with either a tACS or SHAM stimulation applied to the primary motor cortex (M1) simultaneously. For the fatiguing contraction, the participants matched an isometric target force of 20% of the maximum voluntary contraction (MVC) force until task failure. Pre- and post-MVCs were performed to quantify the force decline due to fatigue. Accordingly, the dependent variables were the TTF and MVC force decline as well as the average EMG activity, force error, and standard deviation (SD) of force during the fatiguing contractions. The results indicate that there were no significant differences in any of the dependent variables between the tACS and SHAM conditions (p value range: 0.256–0.820). These findings suggest that tACS does not increase the TTF during fatiguing contractions in young adults.