bluuebunny/crossref_metadata_2025 huggingface dataset:
APLIKASI MONITORING SISTEM ABSENSI SIDIK JARI SEBAGAI PENDUKUNG PEMBAYARAN BIAYA PEGAWAI TERPUSAT DENGAN SAP
Authors: Rafika Ageng Setiani , Budiarto Mukti , Budianto Wahyu
Published: May 2020

Monitoring Data on the presence of Usig Fingerprints is a strategic approach towards improving the discipline of employees, to achieve improved discipline such officials then needed an application monitoring system using Fingerprint time & attendance data are not effectively separated the human, infrastructure and overall management system for centralized employee fee payment process with SAP/ERP as expected and did not experience any delay in payment. Attendance System Monitoring application is capable of helping the PA (Payrool Administration) in favour of an increase in the accuracy of the data centralized employee fee payments with SAP can be realized should be supported by a reliable Technology Informatics infrastructure. Attendance Monitoring System application using the Fingerprint are created in this journal is an application programming examples that use the programming language visual basic 6.0 and SQL Server database, which is expected to help resolve problems existing in HUMAN RESOURCES in General and the ADM. Personnel in particular. The design of monitoring data on the presence of using fingerprints only handles input from someone who has been registered in the database, whereas the output that is displayed showing the reports relating to the attendance reports to be used as the supporter of the accuracy of the data centralized employee fee payments with SAP.
CONTENT MANAGEMENT SYSTEM ZPRENEUR IN SUPPORT OF ENTREPRENEURSHIP ILEARNING AT PERGURUAN TINGGI RAHARJA
Authors: Aini Qurotul , Handayani Indri , Seto Cahyo Anggoro
Published: May 2020

Students are not only provided a knowledge of soft skills for studying in lecture bench, but also trained in how to build the entrepreneurial spirits to face the competition of globalization. Each college also has a different method in building students entrepreneurial spirits. In the practice of entrepreneurship courses in Perguruan Tinggi Raharja, the students perform entrepreneurial activity in a way that is still very conventional. Where they are assigned to sell a product within the scope of the campus. Seeing how the conventional sales process and marketing reach is limited, then digagaskanlah an alternative solution to the entrepreneurial activities by utilizing the development of information and communication technology. Zpreneur content management system is a good solution for students perform entrepreneurial activities. Where zpreneur provide a forum for students to sell their products online. Each student can have their own online store on zpreneur system. That way, they can market their products online, and of course develop their market share not only in the scope of the campus.
WIRELESS SURVEILLANCE CAMERA WITH AMC (AUTOMATIC MOTION CAPTURES) SYSTEM
Authors: Rafika Ageng Setiani , Saefullah Asep , Gozali Andri Ahmad
Published: May 2020

Nowadays, utilizing surviellence cameras was very popular because is user friendly and it has many usefull as a monitoring and as a part of security system at home, institution, office, etc. The existing surviellence camera using computer as a control system and as a media storage so it’s  need a energy and high cost  for implementation. Surveillance cameras are designed using the Raspberry Pi B, PIR sensor, webcam Logitech C 170, SD Card, Twitter, Raspbian operating system, and python programming. Automatic input data is performed by PIR sensor that will be detected human around of range area frequently. Output 3.3 - 5 volt (High) will be generated when sensor detected the human around of range area. Otherwise 0 volts output ( Low) will be generated when sensor not detected the human around of range area frequently. Then, The input will be processing by Raspberry Pi using python programming that has made to a command to capture or not.implemetation outcome  is a surveillance camera that will capture image in (.jpg) format when the input system detected human around of range frequently. Capture result will save to SD Card that have integrated system. Then the sistem will provide immediately report the situation in a real-time notification through a twitter application device.
Para onde Caminha a Democracia Brasileira: Após a Primavera de 2013 e o Impeachment da ex-Presidente Dilma Rousseff
Authors: Aquino Vicente
Published: May 2020

Democracy is one of the most fascinating themes in the political grounds of the contemporary society. Globalization and the progress of the capitalist system were majorly responsible for the deliberation about the genuine characteristics that this concept should reach. Whether as a civilization model or a form of government, democracy is remembered when values such as equality and freedom are jeopardized. Since Ancient Greece, democracy has been exalted and worshiped as a government and civilization regime, but the concepts of nation, its representation and the state, as they evolved, impacted on the meaning of the term in the societies. Some challenges peculiar to Latin America were responsible for the semantic move of a democratic system, that, beyond representing the people’s will, shall rule guided by mutual interest. The linking of the concept of democracy solely with a political regime meant that the exercise of this value was placed in the hands of political representatives. In Brazil, the crisis of representativeness culminates in the manifestoes that occurred throughout the country in 2013, which apparently reveal a new period of Brazilian democracy. In this sense, the present article analyzes how the Brazilian Spring of 2013 influenced the impeachment of the former president Dilma Rousseff and tries to address the paths that the Brazilian democracy intends to follow.
O Movimento Sem Terra e os Governos Lula: Da Utopia e do Pragmatismo
Authors: Bernardes Bruno
Published: May 2020

The article explains the relationship between the Landless Rural Workers Movement (MST) and the Workers’ Party (PT) in Brazil. It is argued that the strategy of the MST within the PT’s political party struggle was built in a pragmatic way, being a significant partner in the victory of Lula da Silva in the presidential elections of 2002. In the Brazilian context the relation between parties and social movements has been quoted as one of dependence. However, by analyzing the relationship of the MST with the governments of Lula da Silva, it can be concluded that the latter managed its relationship with the PT through a pragmatic-ideological insertion.
Analisis Kadar Siklamat Dengan Metode Kromatografi Cair Kinerja Tinggi Pada Minuman Jajanan Sekolah Di Kota Palu
Authors: Maudu Rusli , Hafid Fahmi , Dewi Susetiyany Ichsan
Published: May 2020

Siklamat adalah pemanis buatan yang diproses secara kimiawi. Siklamat tidak boleh dipakai pada hasil pangan untuk bayi, anak batita, ibu hamil serta ibu menyusui. Batas maksimum penggunaan Siklamat adalah 0-11 mg/kg berat badan. Tujuan penelitian untuk mengetahui kadar siklamat pada minuman jajanan sekolah di Kota Palu, Penelitian ini menggunakan metode observasi di 8 (delapan) lokasi di Kota Palu pada tanggal 8-24 Agustus 2018. Analisis kadar Siklamat di Balai Pengawas Obat dan Makanan di Palu. Sampel sebanyak 20 minuman jajajan diperoleh dengan cara accidental sampling. Metode analisis dengan kromatografi cair kinerja tinggi (KCKT). Hasil penelitian menunjukkan bahwa kadar siklamat pada minuman jajanan sekolah di Kota Palu yang melebihi ambang batas perka BPOM < 350 mg/Kg sebanyak 35% dengan kadar 514,63 hingg 2963,43 mg/Kg. Disarankan agar melakukan penelitan lebih lanjut tentang pengetahuan, sikap dan perilaku penjaja minuman dilokasi yang melebihi ambang batas perka BPOM <350 mg/Kg serta edukasi tentang gerakan masyarakat sadar pangan
Parenting Skill Meningkatkan Pengetahuan Orangtua tentang Pembentukan Karakter Disiplin Anak Prasekolah
Authors: Setiadi Rizky , Gandini Andi Lis Arming , Kalsum Umi
Published: May 2020

Karakter disiplin anak merupakan hal yang harus dibentuk sejak dini. Orang tua berperan utama dalam membentuk karakter disiplin pada anak, karenanya orang tua harus memiliki pengetahuan dan keterampilan yang memadai untuk melakukannya. Parenting skill merupakan salah satu upaya yang bisa dilakukan untuk dapat meningkatkan pengetahuan orang tua tentang pembentukan karakter disiplin pada anak. Penelitian ini merupakan penelitian analitik eksperimental dengan desain pretest and posttest without control group. Sampel yang diteliti sejumlah 41 ibu yang memiliki anak uia prasekolah terpilih menjadi responden dengan pengumpulan data sebanyak dua kali untuk mengetahui pengetahuan orang tua tentang pembentukan karakter disiplin pada anak preschool. Hasil uji wilcoxon rank test didapatkan adanya peningkatan nilai pengetahuan orang tua tentang pembentukan karakter disiplin pada anak sebelum dan sesudah diberikan pelatihan parenting skill (nilai p: 0.0001). perlu dilakukan upaya-upaya pemberian edukasi teknik parenting skill kepada orang tua untuk lebih meningkatkan pemahaman orang tua tentang pembentukan karakter disiplin pada anak, sehingga dapat tumbuh dan berkembang secara optimal dan memiliki karakter disiplin yang kuat.
Change-Point Detection in Autoregressive Processes via the Cross-Entropy Method
Authors: Ma Lijing , Sofronov Georgy
Published: May 2020

It is very often the case that at some moment a time series process abruptly changes its underlying structure and, therefore, it is very important to accurately detect such change-points. In this problem, which is called a change-point (or break-point) detection problem, we need to find a method that divides the original nonstationary time series into a piecewise stationary segments. In this paper, we develop a flexible method to estimate the unknown number and the locations of change-points in autoregressive time series. In order to find the optimal value of a performance function, which is based on the Minimum Description Length principle, we develop a Cross-Entropy algorithm for the combinatorial optimization problem. Our numerical experiments show that the proposed approach is very efficient in detecting multiple change-points when the underlying process has moderate to substantial variations in the mean and the autocorrelation coefficient. We also apply the proposed method to real data of daily AUD/CNY exchange rate series from 2 January 2018 to 24 March 2020.
Indicators of Efficiency in the Pharmaceutical Management of a Public Health System
Authors: Martínez-Blanes Manuel , Rondán-Cataluña Francisco Javier , Velarde-Gestera José Manuel
Published: May 2020

This practical research arises from a growing interest in offering models that provide a measure of the efficiency of pharmaceutical management in the Public Health System, beyond spending per inhabitant or other similar indices that do not incorporate the effects of the environment. The main objective of the paper is to design a tool that can measure the relative efficiency of health systems, with respect to the rational use of medicament based on its regional socioeconomic context. The first step was to check if it is possible to apply the efficiency and productivity analysis models, widely used in the economy and companies. We have carefully chosen the context factors that are pertinent and influential in the final index: demographic, sanitary, economic, and social. After selecting and ordering the context variables of the different regions of Spain, they are normalized using the index number transformation or ‘distance’ to a reference. The weighted sum method is used to build the synthetic indicators. The main contribution of this paper is to propose a relative efficiency indicator that assumes the context of performance and allows a comparison between health organizations. The methodology offered in this manuscript could assist policymakers to make better decisions in order to enhance the productivity of the public pharmaceutical system, and it makes available feedback about past decisions.
Design of a Real-Time Gas-Exchange Measurement System for Crop Stands in Environmental Scenarios
Authors: Kläring Hans-Peter , Körner Oliver
Published: May 2020

In contrast to conducting measurements on single plants, canopy gas exchange monitored continuously and for large batches of plants can give high-value data for crop physiological models. To this end, a system including eight airtight greenhouse cabins with a ground area of 28.8 m2 and a volume of 107.8 m3 each was designed for measuring the CO2 and H2O gas exchange of crop stands following the general principle of semi-open chambers. The measuring facility consists of a set of mass flow meters allowing air exchange rates between 0.5 h−1 and 19 h−1 (i.e., m3 gas per m3 greenhouse air per hour) and CO2 supply rates up to 4 L min−1 (i.e., ca. 14.9 g m−2 greenhouse h−1) and sensors for measuring the concentrations of CO2 and H2O. There are four separated belowground troughs per cabin for the root environment that can be operated as individual gas exchange chambers measuring the belowground gas exchange for example root zone respiration. This paper outlines a demonstration of the possibilities and constraints for measuring crop gas exchange in combination with crop model validation for larger crop stands under various conditions and discusses them along with examples.
Early-Life Intake of an Isotonic Protein Drink Improves the Gut Microbial Profile of Piglets
Authors: Buzoianu Stefan G. , Firth Ava M. , Putrino CallaBria , Vannucci Fabio
Published: May 2020

A healthy microbial community in the gut of piglets is critical to minimize the negative performance consequences associated with dietary and environmental changes that occur at weaning. Tonisity Px, an isotonic protein drink, is a potential alternative to balance the gut microbiota as it contains key ingredients for nourishing the small intestine. In the present study, 16 litters comprising 161 piglets were randomly allocated to a group to which Tonisity Px was provided from days 2 to 8 of age (TPX group) or to a control group, to which no Tonisity Px was provided. The TPX group also received Tonisity Px in the 3 days before and after weaning. At days 9, 17, and 30 of age, fecal and ileum samples were collected from piglets belonging to both groups and analyzed using 16S rRNA gene sequencing, semiquantitative PCR of Rotavirus serogroups, and semiquantitative Escherichia coli culture. Overall, Tonisity Px increased the abundance of beneficial bacterial populations (Lactobacillus and Bacteroides species) and reduced potentially pathogenic bacterial populations (E. coli and Prevotellaceae), in both the pre-weaning and post-weaning periods.
Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid
Authors: Podstawski Przemysław , Witarski Wojciech , Szmatoła Tomasz , Bugno-Poniewierska Monika , Ropka-Molik Katarzyna
Published: May 2020

Sarcoids are the most common skin neoplasm in the Equidae family. Sarcoids are benign, but may cause severe damage in affected animals. Due to the high risk of post-treatment recurrence and the lack of an effective method of treatment, it is reasonable to perform studies on the molecular aspects of this neoplasm. Therefore, the present studies analyzed five genes (cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3 and vimentin) related to cell mobility and invasion traits. Primary healthy fibroblasts and sarcoid cells were obtained from skin biopsies. Cell lines were cultured in two different medium types with different concentrations of foetal bovine serum (10% and 0.5% FBS) to study its influence on the analyzed genes. Gene expression was measured using the real-time PCR method. The results showed significant differences in two genes (coronin and vimentin) depending on culture conditions. In conclusion, the results enabled finding two new genes, related to cell motility and invasion traits, in which gene expression is deregulated. Results of the study may put new knowledge into the complexity of the genetic background of this disease and show the importance of further analysis on this subject.
Fallow Deer (Dama dama) as a Reservoir of Shiga Toxin-Producing Escherichia coli (STEC)
Authors: Szczerba-Turek Anna , Kordas Bernard
Published: May 2020

Shiga toxin-producing Escherichia (E.) coli (STEC) are responsible for the outbreaks of serious diseases in humans. Only a few reports on fallow deer as a reservoir of foodborne pathogens have been published to date. The purpose of this study was to determine the occurrence of STEC strains in the fallow deer population in Poland. In all, 94 fallow deer swabs were tested. Polymerase chain reaction (PCR) was performed to detect the virulence profile of stx1, stx2 and eae or aggR genes, to identify the subtypes of stx1 and stx2 genes and to perform O and H serotyping. STEC and attaching and effacing (AE)-STEC were identified in 13 isolates (13.83%). The most hazardous virulence profile was detected in three strains, namely stx2d serotype O103:HNM, eae/stx1a serotype O26:HNM and eae/stx1a serotype O157:H7. The predominant stx gene was stx2, which was identified in 76.92% of isolates. E. coli O157 was detected in 4/94 (4.26%). Other E. coli serogroups, O26, O103, O111 and O145, were identified in 14/94 fallow deer (14.89%). The present findings suggest that fallow deer are carriers of STEC/AE-STEC that are potentially pathogenic to humans.
Understanding Temporal Social Dynamics in Zoo Animal Management: An Elephant Case Study
Authors: Williams Ellen , Bremner-Harrison Samantha , Hall Carol , Carter Anne
Published: May 2020

Zoo animal management procedures which lead to changes to social groups can cause disruption in social hierarchies and the temporary breakdown of social relationships. Animals have different roles in social networks. Understanding individual positions in social networks is important for effective management and ensuring positive welfare for all animals. Using elephants as a case study, the aim of this research was to investigate temporal social dynamics in zoo animals. Behavioural data were collected between January 2016 and February 2017 from 10 African and 22 Asian elephants housed at seven zoos and safari parks in the UK and Ireland. Social interactions were defined as positive physical, positive non-physical, negative physical or negative non-physical. Social network analysis explored social relationships including the fluidity of networks over time and dyadic reciprocity. Social interaction networks were found to be fluid but did not follow a seasonal pattern. Positive interaction networks tended to include the entire social group whereas negative interactions were restricted to specific individuals. Unbalanced ties were observed within dyads, suggesting potential inequalities in relationships. This could impact on individual experiences and welfare. This research highlights subtle temporal dynamics in zoo elephants with the potential for species-level differences. Similar temporal dynamics may also be present in other socially housed zoo species. This research thus provides evidence for the importance of understanding the social networks of zoo animals over longer periods of time. Understanding social networks enables pro-active and evidence-based management approaches. Further research should seek to identify the minimum sampling efforts for social networks in a range of species, to enable the implementation of regular monitoring of social networks and thus improve the welfare of social species under human care.
Molecular Characterization, Expression Profiling, and SNP Analysis of the Porcine RNF20 Gene
Authors: Zhao Ying , Yang Shulin , Wang Yanfang , Tao Cong
Published: May 2020

Fat deposition is considered an economically important trait in pig breeding programs. Ring finger protein 20 (RNF20), an E3 ubiquitin protein ligase, has been shown to be closely involved in adipogenesis in mice, suggesting its conserved role in pigs. In this study, we obtained the exon sequences of the porcine RNF20 gene and characterized its molecular sequence. The porcine RNF20 gene contains 20 exons that encode 975 amino acids, and its RING domain is highly conserved across different species. Western blot analysis revealed that RNF20 was widely expressed, especially in various fat depots, and the level of H2B monoubiquitination (H2Bub) was highly consistent. Eight potential SNPs were detected by sequencing pooled PCR fragments. PCR–RFLP was developed to detect a single nucleotide polymorphism (A-1027G) in exon 1, and the allele frequency differences were examined in four pig breeds. The G allele was predominant in these pigs. Association analysis between (A-1027G) and the backfat thickness of three commercial pig breeds was performed, but no significant association was found. Taken together, these results enabled us to undertake the molecular characterization, expression profiling, and SNP analysis of the porcine RNF20 gene.
Combination of Bacillus licheniformis and Salinomycin: Effect on the Growth Performance and GIT Microbial Populations of Broiler Chickens
Authors: Trela Jacek , Kierończyk Bartosz , Hautekiet Veerle , Józefiak Damian
Published: May 2020

The aim of the study was to investigate the effect of Bacillus licheniformis and salinomycin supplementation in broiler diets as individual factors or in combination on the growth performance, GIT morphometry, and microbiota populations. Four hundred one-day-old Ross 308 chicks were randomly distributed to four dietary treatments (10 replicates, 10 birds each). The following treatments were applied: NC—no additives; NC + SAL—salinomycin addition (60 mg/kg diet), NC + PRO—B. licheniformis DSM 28710 preparation (1.6 × 109 CFU/kg; 500 mg/kg diet), and NC + SAL + PRO—combination of salinomycin and B. licheniformis. Probiotic administration resulted in improvement (p < 0.05) of the performance parameters, including body weight gain (1–10 d, and 11–22 d) and feed conversion ratio (11–22 d, 1–36 d). An interaction (p < 0.05) between experimental factors was observed in terms of lower pH values in the crop (tendency, p = 0.053) and ceca. Both factors lowered the alpha diversity and Enterobacteriaceae and promoted Bacillaceae communities in the jejunum (p < 0.05). Interactions were also observed in terms of reducing Clostridiaceae in the ceca. In conclusion, the combined use of B. licheniformis and salinomycin in broilers’ diets had beneficial effects.
Animal-Based Measures for the On-Farm Welfare Assessment of Geese
Authors: Tremolada Carlo , Bielińska Halina , Minero Michela , Ferrante Valentina , Canali Elisabetta , Barbieri Sara
Published: May 2020

Currently, no specific animal-based measures (ABMs) protocols are available for geese in commercial meat production systems. Following a critical review of the literature and consultation of experts, seven ABMs, potentially valid and feasible for the on-farm welfare assessment of geese, were identified and then tested in 12 farms in Poland to assess their inter-observer reliability. Two observers conducted the assessment, which was divided into two phases. First, a handling test assessed the human–animal relationship (HAR), and a 100% inter-observer reliability was achieved by the observers when evaluating the attitudes of stockpeople and the reactions of geese to humans. Next, an animal inspection was conducted, and the observers simultaneously and independently visually evaluated 100 randomly selected geese per farm and assessed whether the selected ABMs could be identified. In terms of inter-observer reliability, high correlation coefficients were found for plumage dirtiness (ρ = 0.745; p < 0.01), twisted wings (ρ = 0.890; p < 0.001), and broken/twisted wings (ρ = 0.858; p < 0.001). The results showed that plumage dirtiness, twisted wings, and broken/twisted wings are valid and reliable measures. Further research should address the reliability of ABMs of geese in other types of production systems.
Influence of the Casein Composite Genotype on Milk Quality and Coagulation Properties in the Endangered Agerolese Cattle Breed
Authors: Albarella Sara , Selvaggi Maria , D’Anza Emanuele , Cosenza Gianfranco , Caira Simonetta , Scaloni Andrea , Fontana Annunziata , Peretti Vincenzo , Ciotola Francesca
Published: May 2020

The aim of this study was the characterization of CSN1S1, CSN2 and CSN3 genetic variability in Agerolese cattle, and the investigation of the effect of casein composite genotypes (CSN1S1, CSN2 and CSN3) on quality and coagulation traits of the corresponding milk. To these purposes, blood and milk from 84 cows were sampled and analysed. Allele frequencies at CSN2 and CSN3 revealed no Hardy–Weinberg equilibrium in the population with a prevalence of allele A2 for CSN2 and allele B for CSN3. BBA1A2AB and BBA2A2AB composite genotypes were the most common in the population. BBA1A2AB showed a higher total solids and fat content (12.70 ± 0.16 and 3.93 ± 0.10, respectively), while BBA2A2BB showed the best coagulation properties (RCT 12.62 ± 0.81; k20 5.84 ± 0.37; a30 23.72 ± 1.10). Interestingly, the A2 allele of CSN2 was very widespread in the population; thus, it will be intriguing to verify if A2A2 Agerolese cattle milk and the derived cheese may have better nutraceutical characteristics.
An Advanced Decision Support Platform in Energy Management to Increase Energy Efficiency for Small and Medium Enterprises
Authors: Grigoraș Gheorghe , Neagu Bogdan-Constantin
Published: May 2020

The paper presents a new vision on the energy consumption management in the case of the small and medium enterprises (SMEs), integrated into an advanced decision support platform, with technical and economic benefits on increasing the energy efficiency, with four modules for database management, profiling, forecasting, and production scheduling. Inside each module, artificial intelligence and data mining techniques were proposed to remove the uncertainties regarding the dynamic of technological flows. Thus, the data management module includes the data mining techniques, that extract the technical details on the energy consumption needed in the development of production scheduling strategies, the profiling module uses an original approach based on clustering techniques to determine the typical energy consumption profiles required in the optimal planning of the activities, the forecasting module contains a new approach based on an expert system to forecast the total energy consumption of the SMEs, and production scheduling module integrates a heuristic optimization method to obtain the optimal solutions in flattening the energy consumption profile. The testing was done for a small enterprise from Romania, belonging to the domain of trade and repair of vehicles. The obtained results highlighted the advantages of the proposed decision support platform on the decrease in the intensity of energy consumption per unit of product, reduction of the purchase costs, and modification of the impact for which energy bills have on the operational costs.
Trapping Entomopathogenic Fungi from Vine Terroir Soil Samples with Insect Baits for Controlling Serious Pests
Authors: Mantzoukas Spiridon , Lagogiannis Ioannis , Ntoukas Aristeidis , Eliopoulos Panagiotis A. , Kouretas Demetrios , Karpouzas Dimitrios G. , Poulas Konstantinos
Published: May 2020

Fungi are eukaryotic microorganisms with many forms and certain demands about the soil they colonize, which reveal the conditions that prevail therein. Although they can often kill pests without damaging plants or humans, they are not widely used as pesticides as they are not easy to handle and require much time before they act. Nevertheless, insects can be used as baits to trap these entomopathogenic fungi, giving information about their distribution within the terroir. The purpose of this study was to investigate the soil distribution of entomopathogenic fungi species in soil samples from two vine soil terroirs. Nine fungi were identified in total, but M. anisopliae and B. bassiana showed the greatest frequency, which was expressed by difference in adult bait mortality among species. These fungi had not been previously documented in vine terroirs.
i-Yard 2.0: Integration of Sustainability into a Net-Zero Energy House
Authors: Jin Yichun , Li Junjie , Wu Wei
Published: May 2020

This research introduces a residential net-zero energy house named i-Yard 2.0, which was built by a team from Beijing Jiaotong University for the 2018 Solar Decathlon China competition. The concept was based on the needs of an aging population and achieves energy self-sufficiency through both active (i.e., solar energy) and passive design strategies. With the growing recognition of the need for better environmental protection, green building strategies have become mainstream in building development. A building’s energy balance is one of the most important indexes for assessing green buildings. The i-Yard 2.0 adopts an integrated design strategy with a sustainable development background. It takes a senior citizen-oriented design as the starting point and innovates in aspects such as community modeling, building strategies, passive spatial planning, the energy and building environment, and intelligent building control. The community comprises a new residential model called “cooperative living.” The building strategy adopts a modular assembly approach in order to achieve rapid construction suitable for this type of competition. The passive spatial plan uses the notion of the courtyard as a green core to regulate the microclimate. The building environment achieves net-zero energy by improving active energy access and reducing passive energy consumption. The internet control model was designed to incorporate intelligent building control. The i-Yard 2.0 provides not only a new form of senior residential housing for developing areas, it also provides a novel and worthy reference for net-zero energy housing in China.
Mechanical Characteristics and Failure Mode of Asphalt Concrete for Ballastless Track Substructure Based on In Situ Tests
Authors: Fu Qinghong , Chen Xianhua , Cai Degou , Lou Liangwei
Published: May 2020

Asphalt concrete paved on the surface of a roadbed as a ballastless track substructure has an excellent waterproofing and vibration attenuation performance. However, the mechanical characteristics and the failure mode of this structure under the actions of a cyclic train load and ambient air temperature changes are still unclear. Therefore, a test section of an asphalt concrete substructure was constructed based on a high-speed railway ballastless track project in north China. In situ forced vibration tests and temperature-induced deformation monitoring tests were performed to investigate the mechanical responses of the asphalt concrete, respectively. Test results show that the bottom of the asphalt concrete layer is in the tensile state under the action of the cyclic train load. The surface of the asphalt concrete in contact with the base plate is subjected to tensile stress near the expansion joint under the action of the negative temperature gradient. Changes in the ambient temperature lead to more significant mechanical responses of the asphalt concrete substructure than the cyclic train load, especially near the expansion joint of the base plate. Therefore, the passive tensile failure mode may occur near the expansion joint of the base plate. However, it has also proved that setting isolation layers under the base plate near the expansion joint is an effective method to significantly reduce responses near the expansion joint in this research.
Disappearance on Display: Vanished Torah Arks in Medieval Synagogues and Their Presentation
Authors: Stürzebecher Maria
Published: May 2020

In planning the museum in the medieval Synagogue in Erfurt more than 10 years ago, there was one big problem: the building had gone through many changes over the centuries, which wiped out almost all traces of the synagogue’s use. The Bimah was destroyed, presumably during the pogrom on 21 March 1349. By converting the former synagogue into a warehouse (following the pogrom), a big gateway was inserted into the eastern wall, at the place of the former Torah Ark. Only the light cornice is still recognizable on the synagogue’s walls. To find an adequate solution for displaying the vanished Torah Ark in the Old Synagogue in Erfurt, we compared examples in other locations in Europe, suggesting the presentation eventually chosen for the Old Synagogue of Erfurt.
The Record-Breaking High Temperature over Europe in June of 2019
Authors: Zhao Wei , Zhou Ningfang , Chen Shangfeng
Published: May 2020

Observational and reanalysis data show that the surface air temperature (SAT) over most parts of Europe in June of 2019 broke the highest temperature on record. In this study, we investigate the factors for the formation of this record-breaking high temperature over Europe, focusing on the role of atmospheric circulation anomalies. A strong anomalous anticyclone appeared over Europe, with a quasi-barotropic vertical structure. On one hand, the downward motion anomalies associated with this anomalous anticyclone led to less cloud cover and an increase in downward shortwave radiation, which contributed to the SAT warming over Europe. On the other hand, southerly wind anomalies to the west side of the anomalous anticyclone also resulted in SAT warming via carrying warmer and wetter air northward from lower latitudes. The formation of the anticyclonic anomaly over Europe in June of 2019 was closely related to an atmospheric wave train propagating eastward from the mid-high latitudes of the North Atlantic to Eurasia. The atmospheric wave train over the North Atlantic–Eurasia region is suggested to be mainly related to the Atlantic–Eurasia teleconnection pattern. Further analysis indicates that a decrease in the local soil moisture over Europe may also have escalated the surface temperature warming through a positive land–atmosphere feedback.
SSH-Aerosol v1.1: A Modular Box Model to Simulate the Evolution of Primary and Secondary Aerosols
Authors: Sartelet Karine , Couvidat Florian , Wang Zhizhao , Flageul Cédric , Kim Youngseob
Published: May 2020

Particles are emitted by different sources and are also formed in the atmosphere. Despite the large impact of atmospheric particles on health and climate, large uncertainties remain concerning their representation in models. To reduce these uncertainties as much as possible, a representation of the main processes involved in aerosol dynamics and chemistry is necessary. For that purpose, SSH-aerosol was developed to represent the evolution of the mass and number concentrations of primary and secondary particles, across different scales, using state-of-the-art modules, taking into account processes that are usually not considered in air-quality or climate modelling. For example, the particle mixing state and the growth of ultra-fine particles are taken into account in the aerosol dynamics, the affinity of semi-volatile organic compounds with water and viscosity are taken into account in the partitioning between the gas and particle phases of organics and the formation of extremely low-volatility organic compounds from biogenic precursors is represented. SSH-aerosol is modular and can be used with different levels of complexity. It may be used as standalone to analyse chamber measurements. It is also designed to be easily coupled to 3D models, adapting the level of complexity to the spatial scale studied.
Seasonal Asian Dust Forecasting Using GloSea5-ADAM
Authors: Ryoo Sang-Boom , Lim Yun-Kyu , Park Young-San
Published: May 2020

The springtime dust events in Northeast Asia pose many economic, social, and health-related risks. Statistical models in the forecasting of seasonal dust events do not fully account for environmental variations in dust sources due to climate change. The Korea Meteorological Administration (KMA) recently developed the GloSea5-ADAM, a numerically based seasonal dust forecasting model, by incorporating the Asian Dust and Aerosol Model (ADAM)’s emission algorithm into Global Seasonal Forecasting Model version 5 (GloSea5). The performance of GloSea5 and GloSea5-ADAM in forecasting seasonal Asian dust events in source (China) and leeward (South Korea) regions was compared. The GloSea5-ADAM solved the limitations of GloSea5, which were mainly attributable to GloSea5′s low bare-soil fraction, and successfully simulated 2017 springtime dust emissions over Northeast Asia. The results show that GloSea5-ADAM’s 2017 and 2018 forecasts were consistent with surface PM10 mass concentrations observed in China and South Korea, while there was a large gap in 2019. This study shows that the geographical distribution and physical properties of soil in dust source regions are important. The GloSea5-ADAM model is only a temporary solution and is limited in its applicability to Northeast Asia; therefore, a globally applicable dust emission algorithm that considers a wide variety of soil properties must be developed.
Transcriptomics of Mature Rice (Oryza Sativa L. Koshihikari) Seed under Hot Conditions by DNA Microarray Analyses
Authors: Bakku Ranjith Kumar , Rakwal Randeep , Shibato Junko , Cho Kyoungwon , Kikuchi Soshi , Yonekura Masami , Sarkar Abhijit , Shioda Seiji , Agrawal Ganesh Kumar
Published: May 2020

Higher temperature conditions during the final stages of rice seed development (seed filling and maturation) are known to cause damage to both rice yield and rice kernel quality. The western and central parts of Japan especially have seen record high temperatures during the past decade, resulting in the decrease of rice kernel quality. In this study, we looked at the rice harvested from a town in the central Kanto-plains (Japan) in 2010. The daytime temperatures were above the critical limits ranging from 34 to 38 °C at the final stages of seed development and maturity allowing us to investigate high-temperature effects in the actual field condition. Three sets of dry mature rice seeds (commercial), each with specific quality standards, were obtained from Japan Agriculture (JA Zen-Noh) branch in Ami-town of Ibaraki Prefecture in September 2010: grade 1 (top quality, labeled as Y1), grade 2 (medium quality, labeled as Y2), and grade 3 (out-of-grade or low quality, labeled as Y3). The research objective was to examine particular alterations in genome-wide gene expression in grade 2 (Y2) and grade 3 (Y3) seeds compared to grade 1 (Y1). We followed the high-temperature spike using a high-throughput omics-approach DNA microarray (Agilent 4 × 44 K rice oligo DNA chip) in conjunction with MapMan bioinformatics analysis. As expected, rice seed quality analysis revealed low quality in Y3 > Y2 over Y1 in taste, amylose, protein, and fatty acid degree, but not in water content. Differentially expressed gene (DEG) analysis from the transcriptomic profiling data revealed that there are more than one hundred upregulated (124 and 373) and downregulated (106 and 129) genes in Y2 (grade 2 rice seed) and Y3 (grade 3 rice seed), respectively. Bioinformatic analysis of DEGs selected as highly regulated differentially expressed (HRDE) genes revealed changes in function of genes related to metabolism, defense/stress response, fatty acid biosynthesis, and hormones. This research provides, for the first time, the seed transcriptome profile for the classified low grades (grade 2, and out-of-grade; i.e., grade 3) of rice under high-temperature stress condition.
Porous Carbon Monoliths Made from Cellulose and Starch
Authors: Klepel Olaf , Danneberg Nina
Published: May 2020

Porous carbon monoliths can be used as key components in a variety of applications, such as energy storage, adsorption and catalysis. The preparation of porous carbon monoliths suffers from several limitations, e.g., time-consuming synthesis steps, the use of hazardous chemicals, limited porosity or mechanical stability. This paper describes the investigation of a simple synthesis route to produce porous carbon monoliths from sustainable carbon precursors. Mixtures from different kinds of cellulose and starch, respectively, have been used as the carbon precursor. Fundamental features of porous monoliths, i.e., the porosity and the mechanic stability, respectively, have been investigated in dependence on the composition of the precursor mixtures. First attempts to explain the observed behavior have already been made.
Cold-Atmospheric Plasma Induces Tumor Cell Death in Preclinical In Vivo and In Vitro Models of Human Cholangiocarcinoma
Authors: Vaquero Javier , Judée Florian , Vallette Marie , Decauchy Henri , Arbelaiz Ander , Aoudjehane Lynda , Scatton Olivier , Gonzalez-Sanchez Ester , Merabtene Fatiha , Augustin Jérémy , Housset Chantal , Dufour Thierry , Fouassier Laura
Published: May 2020

Through the last decade, cold atmospheric plasma (CAP) has emerged as an innovative therapeutic option for cancer treatment. Recently, we have set up a potentially safe atmospheric pressure plasma jet device that displays antitumoral properties in a preclinical model of cholangiocarcinoma (CCA), a rare and very aggressive cancer emerging from the biliary tree with few efficient treatments. In the present study, we aimed at deciphering the molecular mechanisms underlying the antitumor effects of CAP towards CCA in both an in vivo and in vitro context. In vivo, using subcutaneous xenografts into immunocompromised mice, CAP treatment of CCA induced DNA lesions and tumor cell apoptosis, as evaluated by 8-oxoguanine and cleaved caspase-3 immunohistochemistry, respectively. The analysis of the tumor microenvironment showed changes in markers related to macrophage polarization. In vitro, the incubation of CCA cells with CAP-treated culture media (i.e., plasma-activated media, PAM) led to a dose response decrease in cell survival. At molecular level, CAP treatment induced double-strand DNA breaks, followed by an increased phosphorylation and activation of the cell cycle master regulators CHK1 and p53, leading to cell cycle arrest and cell death by apoptosis. In conclusion, CAP is a novel therapeutic option to consider for CCA in the future.
Tetraploidization of Immortalized Myoblasts Induced by Cell Fusion Drives Myogenic Sarcoma Development with DMD Deletion
Authors: Merle Candice , Thébault Noémie , LeGuellec Sophie , Baud Jessica , Pérot Gaëlle , Lesluyes Tom , Delespaul Lucile , Lartigue Lydia , Chibon Frédéric
Published: May 2020

Whole-genome doubling is the second most frequent genomic event, after TP53 alterations, in advanced solid tumors and is associated with poor prognosis. Tetraploidization step will lead to aneuploidy and chromosomic rearrangements. The mechanism leading to tetraploid cells is important since endoreplication, abortive cytokinesis and cell fusion could have distinct consequences. Unlike processes based on duplication, cell fusion involves the merging of two different genomes, epigenomes and cellular states. Since it is involved in muscle differentiation, we hypothesized that it could play a role in the oncogenesis of myogenic cancers. Spontaneous hybrids, but not their non-fused immortalized myoblast counterparts they are generated from, induced tumors in mice. Unstable upon fusion, the hybrid genome evolved from initial mitosis to tumors with a highly rearranged genome. This genome remodeling finally produced targeted DMD deletions associated with replicative stress, isoform relocalization and metastatic spreading, exactly as observed in human myogenic sarcomas. In conclusion, these results draw a model of myogenic oncogenesis in which cell fusion and oncogene activation combine to produce pleomorphic aggressive sarcomas.
Acidosis-Induced TGF-β2 Production Promotes Lipid Droplet Formation in Dendritic Cells and Alters Their Potential to Support Anti-Mesothelioma T Cell Response
Authors: Trempolec Natalia , Degavre Charline , Doix Bastien , Brusa Davide , Corbet Cyril , Feron Olivier
Published: May 2020

For poorly immunogenic tumors such as mesothelioma there is an imperious need to understand why antigen-presenting cells such as dendritic cells (DCs) are not prone to supporting the anticancer T cell response. The tumor microenvironment (TME) is thought to be a major contributor to this DC dysfunction. We have reported that the acidic TME component promotes lipid droplet (LD) formation together with epithelial-to-mesenchymal transition in cancer cells through autocrine transforming growth factor-β2 (TGF-β2) signaling. Since TGF-β is also a master regulator of immune tolerance, we have here examined whether acidosis can impede immunostimulatory DC activity. We have found that exposure of mesothelioma cells to acidosis promotes TGF-β2 secretion, which in turn leads to LD accumulation and profound metabolic rewiring in DCs. We have further documented how DCs exposed to the mesothelioma acidic milieu make the anticancer vaccine less efficient in vivo, with a reduced extent of both DC migratory potential and T cell activation. Interestingly, inhibition of TGF-β2 signaling and diacylglycerol O-acyltransferase (DGAT), the last enzyme involved in triglyceride synthesis, led to a significant restoration of DC activity and anticancer immune response. In conclusion, our study has identified that acidic mesothelioma milieu drives DC dysfunction and altered T cell response through pharmacologically reversible TGF-β2-dependent mechanisms.
Genome-Wide Association Meta-Analysis of Single-Nucleotide Polymorphisms and Symptomatic Venous Thromboembolism during Therapy for Acute Lymphoblastic Leukemia and Lymphoma in Caucasian Children
Authors: Mateos Marion K. , Tulstrup Morten , Quinn Michael CJ , Tuckuviene Ruta , Marshall Glenn M. , Gupta Ramneek , Mayoh Chelsea , Wolthers Benjamin O. , Barbaro Pasquale M. , Ruud Ellen , Sutton Rosemary , Huttunen Pasi , Revesz Tamas , Trakymiene Sonata S. , Barbaric Draga , Tedgård Ulf , Giles Jodie E. , Alvaro Frank , Jonsson Olafur G. , Mechinaud Françoise , Saks Kadri , Catchpoole Daniel , Kotecha Rishi S. , Dalla-Pozza Luciano , Chenevix-Trench Georgia , Trahair Toby N. , MacGregor Stuart , Schmiegelow Kjeld
Published: May 2020

Symptomatic venous thromboembolism (VTE) occurs in five percent of children treated for acute lymphoblastic leukemia (ALL), but whether a genetic predisposition exists across different ALL treatment regimens has not been well studied. Methods: We undertook a genome-wide association study (GWAS) meta-analysis for VTE in consecutively treated children in the Nordic/Baltic acute lymphoblastic leukemia 2008 (ALL2008) cohort and the Australian Evaluation of Risk of ALL Treatment-Related Side-Effects (ERASE) cohort. A total of 92 cases and 1481 controls of European ancestry were included. Results: No SNPs reached genome-wide significance (p < 5 × 10−8) in either cohort. Among the top 34 single-nucleotide polymorphisms (SNPs) (p < 1 × 10−6), two loci had concordant effects in both cohorts: ALOX15B (rs1804772) (MAF: 1%; p = 3.95 × 10−7) that influences arachidonic acid metabolism and thus platelet aggregation, and KALRN (rs570684) (MAF: 1%; p = 4.34 × 10−7) that has been previously associated with risk of ischemic stroke, atherosclerosis, and early-onset coronary artery disease. Conclusion: This represents the largest GWAS meta-analysis conducted to date associating SNPs to VTE in children and adolescents treated on childhood ALL protocols. Validation of these findings is needed and may then lead to patient stratification for VTE preventive interventions. As VTE hemostasis involves multiple pathways, a more powerful GWAS is needed to detect combination of variants associated with VTE.