bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Variation at the Sulfonylurea Receptor, Type 2 Diabetes, and Coronary Heart Disease
Authors: Emdin Connor A. , Klarin Derek , Natarajan Pradeep ,   CARDIOGRAM Exome Consortium, Florez Jose C. , Kathiresan Sekar , Khera Amit V.
Published: April 2017

Despite widespread clinical use in the treatment of type 2 diabetes, the impact of sulfonylurea therapy on cardiovascular outcomes remains uncertain. Studies of naturally occurring genetic variation can be used to anticipate the expected clinical consequences of a pharmacological therapy. A common missense variant in the gene encoding a component of the sulfonylurea receptor (ABCC8 p.A1369S) promotes closure of the target channel of sulfonylurea therapy and is associated with increased insulin secretion, thus mimicking the effects of sulfonylurea therapy. Using individual-level data from 120,286 participants in the UK Biobank and summary association results from four large-scale genome-wide association studies, we examined the impact of this variant on cardiometabolic traits, type 2 diabetes, and coronary heart disease. The p.A1369S variant was associated with a significantly lower risk of type 2 diabetes (odds ratio [OR] 0.93; 95% CI 0.91, 0.95; P = 1.2 × 10−11). The variant was associated with increased BMI (+0.062 kg/m2; 95% CI 0.037, 0.086; P = 8.1 × 10−7) but lower waist-to-hip ratio adjusted for BMI, a marker of abdominal fat distribution. Furthermore, p.A1369S was associated with a reduced risk of coronary heart disease (OR 0.98; 95% CI 0.96, 0.99; P = 5.9 × 10−4). These results suggest that, despite a known association with increased weight, long-term sulfonylurea therapy may reduce the risk of coronary heart disease.
INDUKSI 2-METHOXYETHANOL PADA MASA PRENATAL SEBAGAI PENYEBAB KELAINAN OTAK PADA MENCIT
Authors: Darmanto Win , Prihiyantoro Eko , Harmonis Ria
Published: April 2017

2-Methoxyethanol (2-ME) and glycol esters are widely used as a solvents in the industry and plasticizers, and have becomes ubiquitous environmental pollutants. This study were designed to evaluate the malformation of brain development as an impact of 2-ME. Four groups of pregnant mice were injected to 2-ME dose 10 mmol/kg body weight at 7, 13, 15, and 17 days gestation respectively. Control group were injected with sterile water. At 18 days of gestation samples in groups 7 and 13 days gestation were sacrificed. Brain were remove and weight, morfologically and histologically were examined. Samples were divided into five groups, i.e. control group, 7th, 13rd, 15th and 17th days of gestation groups. Samples in 7th and 15th days of gestation groups killed in 18th days of gestation to observed fetal brain defect and decreased of fetal brain weight but some samples to allow delivery of fetuses. Furthermore, the fetuses killed on 0, 5, 10, and 20 days after birth, to take their brain. The result of these study showed that there are decreasing total weight of fetal brains and there are cerebral malformation as impact of 2-ME exposure.
PENGARUH INFUS RIMPANG TEMULAWAK (Curcuma xanthorrhiza) TERHADAP KADAR HEMOGLOBIN DAN JUMLAH ERITROSIT TIKUS PUTIH YANG DIBERI LARUTAN TIMBAL NITRAT [(PbNO3)2]
Authors: Sugiharto Sugiharto
Published: April 2017

The purpose of this experiment was to study the effect of turmeric (Curcuma xanthorrhiza) rhizome infuse to hemoglobin concentration and number of erythrocyte of (PbNO3)2 treated rats. Pb was presumed to be link at sulphyhydril groups that may caused an inhibition to several enzymatic process, such as d-ALAD and heme sintetase which is caused the inhibits of Hb synthesis and erythropoesis. Twenty female rats was used in this experiment. They were divided into five groups, i.e. (A) control (treated with 1 ml aquadest), (B) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml aquadest, (C) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml of 20 percent turmeric rhizome infuse, (D) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml aquadest, (E) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml of 20 percent turmeric rhizome infuse. The treatment was given orally every day (30 days) using a modified syringe. After 30 days of treatment, the blood sample were taken about 2 ml by heart puncture. The Hb concentration was determined using by Cyanmethemoglobin method and number of erythrocyte was counting on Haemocytometer Improved Neubauer. Data were analyzed by Anova and LSD test (a = 0.05). The results of this study shows that 20 percent of turmeric rhizome infuse statistically has a significant effect (P less than 0.05) to increasing of hemoglobin concentration but hasn’t significant to prevent the number of erythrocyte decreasing of [(PbNO3)2] treated.
PENGARUH PEMBERIAN KADMIUM TERHADAP TINGKAT KELANGSUNGAN HIDUP DAN KERUSAKAN STRUKTUR INSANG DAN HEPATOPANKREAS PADA UDANG REGANG [Macrobrachium sintangense (de Man)]
Authors: Soegianto Agoes , Primarastri Nia Adiani , Winarni Dwi
Published: April 2017

The purpose of this research was to find out the impact of cadmium on the structure of gills, hepatopancreas and on survival of shrimp. The shrimp was divided into four groups. Group I (control) was exposed to 0 ppb of cadmium, group II exposed to10 ppb of Cd, group III exposed to 20 ppb of Cd and group IV exposed to 30 ppb of Cd. Three replications were applied in each treatment. Survival rate data were collected everyday, and every two days the water was substituted with new water. The experiment was stoped when 50 percent of shrimps dead. The rest of shrimps were prepared for histological study. All data were subjected on Kolmogorov-Smirnov (distribution test) then continued with ANOVA test and Kruscal-Wallis tests. The result of this experiment showed that structural damage on gills and hepatopancreas increase with increasing cadmium concentration in medium. The lamella of shrimp from control group did not show hyperplasia and necrosis; in second group: 24.02 percent of lamella showed hyperplasia, 2.77 percent necrosis and 28.02 percent vacuolization; third group: 70.01 percent of lamella showed hyperplasia, 20.60 percent necrosis and 48.79 percent vacuolization; fourth group: 32.60 percent of lamella demonstrated hyperplasia, 57.35 percent necrosis and 97.50 percent vacuolization. Increasing the structural damage of gills and hepatopancreas, it cause the decrease on survival rate of shrimp.
INVENTARISASI TUMBUHAN PAKU DI DAERAH MALANG
Authors: Sunarmi Sunarmi , Sarwono Sarwono
Published: April 2017

Inventory is a way to collect the data of plants in a region which usually started by activity of collection, description, identification and ended with classification. The variety of Indonesia flora until today have been discovered totally . This research aimed to know the species of fern plants which grow in Malang area. Research had done in March 2001–February 2002 with descriptive method, explores technique, and documentation. Research subject were all of species of fern plants grow in Malang area. Identification to be conducted by comparing entire characteristic exist in found plant with key identify loaded by species of fern plants in Flora Malesiana and some relevant book. Result of research shows that in Malang found 50 species, 28 genus, 15 family, and 4 class from species of fern plants. Suggested to conduct research of inventory of other plants which exist in Malang area to equip flora in Indonesia
PERAN KOMUNITAS MIKROBA LUMPUR AKTIF DALAM PEROMBAKAN DETERGEN ALKIL SULFONAT LINEAR DAN BENZENA ALKIL SULFONAT
Authors: Sudiana I Made
Published: April 2017

The study was conducted to evaluate the biodegradation of detergent contained Alkyl Sulfonate (LAS) and Benzene Alkyl Sulfonate (BAS) occur under aerobic condition by complex microbial community in activated sludge process. A 1.8 L of sequential batch reactor, and batch experiments were set up to study the characters LAS and BAS biodegradation. Microbial community in Activated Sludge actively brook down LAS and BAS. Biodegradation rate of LAS was higher than that of BAS. The complex chemical molecule structure of ABS could be the reason for slower degradation of BAS. The value of LAS degradation under aerobic condition were indicated by μmax of 0.26–h, Ks = 15.5 mg/L, Vmax = 11.04 mg/L. hour–1and Km = 8.19 mg/L. Whereas for BAS were μmax of 0.22–h, Ks = 25.1 mg/L, Vmax = 12.74 mg/L. hour–1and Km = 8.119 mg/L. Activated sludge process is appropriate technology for removal of removal of LAS and BAS.
PENGOBATAN INFEKSI PARVOVIRUS PADA ANJING
Authors: Sardjana IKW , Kusumawati D.
Published: April 2017

Treatments of canine Parvovirus have already done to 22 dogs. There were 16 dogs, one month to one years of age and 6 dogs two years to seven years of age. The results of the theraphy, were ten dogs survived and twelve dogs died due to Parvovirus infection. Fluid theraphy supported by antibiotic, antiemetic or antacids administrations were essential for Parvovirus infection in dogs. The recovery rate of this dogs was 45 percent.
EFEK EKSTRAK TESTIS TERHADAP JUMLAH IMPLANTASI DAN JUMLAH ANAK PADA MENCIT (Mus musculus)
Authors: Wahyuningsih Sri Puji Astuti , Suhargo Listijani , Kushendarsasi Kushendarsasi
Published: April 2017

Extract of testis contains the testis specific proteins. The testis proteins may raise immune responses. The immune responses are the antibodies against testis specific anti-protein. The reaction between the antibodies and the sperms can inhibit fertilization. This process has contraception effects. This research used 24 female and 20 male mice strain Balb/c. The experiment used the complete random design with 4 concentration treatments (0, 500, 1000, and 2000 μg extracts of testis) and 3 replications. Mice were injected 3 times with time interval 21 days. After 15 days from the rising of vaginal plug, 3 mice were dissected and counted the implantation. The others mice were waited until delivered of the children. The datas were analyzed by ANOVA and LSD (a = 5 percent). The results showed that extract of testis did not influence the amount of implantation at the uterus endometrial, but it decreased the amount of children. The effective concentration of extract testis is 2000 μg. So, this study showed that the intraperitoneum injection of the extract of testis had contraception effects.
EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus)
Authors: Hayati Alifah , Yunaida Binti , Pidada I.B. Rai , Darmanto Win , Winarni Dwi
Published: April 2017

This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.
KAJIAN PENDAHULUAN UJI TOKSISITAS EKSTRAK AIR MISELIA DAN TUBUH BUAH JAMUR SHIITAKE (Lentinus edodes) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)
Authors: NS Noor Erma , Sundari Tri , Susanty Arie Ika , Palupi Dwi Riani Oktavia , Isnaeni Isnaeni , Sukardiman Sukardiman
Published: April 2017

Shiitake mushroom (Lentinus edodes) is one of the wood mushroom types that can be consumed as a food as well as for a medical purpose. Lentinan, a polysaccharide contained in shiitake, is well known for its use on cancer medication. Mycelium of Shiitake mushroom contains lentinan the same as other part of the mushroom like fruity body. Toxicity of the lentinan in mycelium compare to the fruity body has been first conducted by using Brine Shrimp Lethality Test (BST). Using Potato Dextrose Broth media with the growth rate of 3.88 percent did mycelium multiplications. Probit analysis showed that the toxicity of the mushroom’s cap, stem, and mycelium of Shiitake mushrooms is LC50 = 648.76507 mg/ml LC50 = 489.39444 mg/ml, and LC50 = 481.16941 mg/ml respectively.
BIOAKUMULASI LOGAM BERAT Cu OLEH Bacillus sp
Authors: Primaharinastiti Riesta , Poernomo A. Toto , S. Noor Erma
Published: April 2017

The research was conducted to investigate the ability of Bacillus sp in accumulating Cu and how much it can be acumulated. The medium used to growth the bacterium was Nutrient Broth and Atomic Absorption Spectrophotometry methods was used to assay the Cu, both in the cells and medium. The result of this study showed that Bacillus sp incubated in the Nutrient Broth medium containing 10 ppm of Cu, with continuous stirring in the room temperature was able to reduce Cu in the medium 8.912–12.623 percent and accumulate Cu in the cell 0.1149–0.1400 percent/mg cells. Based on this result, it is necessary to develop more studies to find out what factors that influence the accumulation process and to optimize the bioprocess.
BIOTRANSFORMASI ADIPONITRIL OLEH Bacillus licheniformis BA2
Authors: Thontowi Ahmad , Pamuji Eko W. , Sunarko Bambang
Published: April 2017

Adipic acid represents one of the especial materials which used for the synthesis of nylon 6,6,- is a very important material results from polyamide industry. Adiponitrile biotransformation become adipic acid represent an alternative synthesis besides chemically. The purpose of this research was to determine optimum conditions for Bacillus licheniformis BA2 growth for adiponitrile degradation, and also know its pattern. The obtained information, to be expected can be used as reference for scaling up of adipic acid production. B. licheniformis BA2 was able to utilize acetonitrile and adiponitrile as the sole source of carbon and nitrogen. The growth on adiponitrile 120 mM mixture with acetonitrile 30 mM gave higher growth rate and biomass yield than growth on another subsrates. B. licheniformis BA2 have lag phase during 68 hours, logaritmic phase passed by during 104 hours, while stasioner phase just reached by after 172 hours. High-performance liquid chromatography of adiponitrile degradation by crude bacterial revealed a decrease in adiponitrile with the sequential formation of adipamide and adipic acid. Ammonia was also detected by colorimetric procedures. As for adipic acid rendemen at 420 minutes equal to 19.35 percent.
CHARACTERISTICS OF THE PEAT SOIL Bacilllus sp.
Authors: Hidayat Iman
Published: April 2017

Twenty two strains of Bacillus species have been isolated from the peat soil of Tanjung Puting, Central Kalimantan. All strains devided into seventeen groups which characterized using morphology and biochemical tests. All strains have been tested for lipase, amylase, protease, cellulase, and phosphatase activity. Thirteen Bacillus isolates tested gave positif reaction for lipase activity. Bacillus AR 018 was the best isolate for amylase activity, Bacillus AR 001 and Bacillus AR 002 for protease activity, Bacillus AR 009 for cellulase activity. Only one isolate shows positive reaction for phosphatase activity, notably Bacillus AR 007.
IDENTIFIKASI BERAT, DIAMETER, DAN TEBAL DAGING BUAH MELON (Cucumis melo, L.) KULTIVAR ACTION 434 TETRAPLOID AKIBAT PERLAKUAN KOLKISIN
Authors: Anggraito Y. Ulung
Published: April 2017

Indonesian farmers are very dependence on certificated seed from another countries. In the other side the natural resources and men powers very abundance. For these reason it is properly developed the research in agriculture sector, especially on plants breeding. It can be hoped that in the future the dependence on certificated seed from another countries can be minimized. The objective of this research were: (1) to find out the concentration and dipping period which is effective to induce polyploid in musk melon plant, (2) identify the weight, diameter, dan flesh thickness of tetraploid musk melon as result of colchicines treatment. The sample of this research was Action 434 musk melon cultivar, product of Chia-Thai Seed, Thailand. The number of sample was 480 plants, which plants on field randomly. There were four colchicines concentration as an independent variable: 0.0 percent, 0.05 percent, 0.10 percent and 0.2 percent. The dipping period were 12, 16, 20, and 24 hours for each concentration respectively. Completely Random Design was used in three replications. Data measurement were analyzed with Two Way ANOVA, DMRT, and LSD. From this research can be concluded that: (1) 0.2 percent colchicine is the most effective concentration to induce polyploid on musk melon, with dipping period effective varied from 16–24 hours, (2) there are changes in weight, diameter, and flesh thickness characters, with the increased tendency of each character in definite norm.
8-HIDROKSI-DEOKSIGUANOSIN SEBAGAI SALAH SATU INDIKATOR INFERTILITAS PRIA
Authors: Sudjarwo Sudjarwo
Published: April 2017

DNA in human’s sperm is located in nucleus (nDNA) and mitochondria (mtDNA). DNA oxidation is indicated from high concentration of 8-OH-dG. It is very important to identify factors that can cause genetic dysfunction risk responsible for genetic changes. 8-OH-dG compound are a promutagenic of DNA dysfunction of deoxyguanosine oxidation by oxygen radical. ROS is an oxidation, which in high concentration can oxidize DNA. Guanine is a nucleotide, which prone to oxidation, with 8-OH-dG as the end product. Detection of 8-OH-dG can be done by HPLC method. These results indicated that there were significant differences (p less than 0.01) in detection of 8-OH-dG in sperm, between normospermia with asthenospermia and normospermia with oligospermia. Correlation (r) of 8-OH-dG with motile spermatozoa is – 0.7111. Immotile spermatozoa caused by oxidation or DNA damage are shown by high concentration of 8-OH-dG. In normal physical condition, sperm produces oxidant. High oxidant is toxic to sperm, which influence their motility. Sperms motility is one of factors causing male infertility.
PERBANDINGAN PENINGKATAN BERAT BADAN ANAK MENCIT YANG DIINDUKSI OLEH PEMBERIAN INFUS DAUN PEPAYA DAN DAUN KATU
Authors: Pidada IB. Rai
Published: April 2017

A comparative study of increasing on body weight of mice ,s offsprings caused by leaf of papaya (Carica pepaya L.) and katu (Sauropus androgynus (L). Merr) were carried out under laboratory conditions. The study was designed using totally 50 mice post partum (PP) which devided into five groups of treatment consistent 10 females mice post partum of each group. The groups were: The control group, group gave orally in 0.5 ml aquadest, 10 percent/0.5 ml, and 20 percent/0.5 ml leaf infuse of papaya and 10 percent/0.5 ml and 20 percent/0.5 ml leaf infuse of katu respectively. The treatment were started on day 3rd to 16th of lactation period. The sample were observed on day 4th, 6th , 8th, 10th, 12nd, 14th, and 16th of lactation period. The data were analyzed by ANOVA and LSD test (α = 0.05). The result of study showed that the increasing of body weight of offsprings were significantly different amongs of five groups. Between the control groups and katu groups was not occurred significantly different. The conclusion of this study is papaya groups more right than katu groups to induce an increasing on body weight of mice,s offsprings.
EXISTENCE OF Stelechocarpus burahol (Bl.) Hook.F. & Th. IN WILDERNESS ZONE, BANDE ALIT RESORT, MERU BETIRI NATIONAL PARK
Authors: Umiyah Umiyah
Published: April 2017

Many researchers (Botanists, Systematists, Taxonomists, etc.), especially who concern in plant conservation are afraid about the existence of Stelechocarpus burahol (Bl.) Hook.f. and Th., family Annonaceae. Because, the plant is becoming rare and facing extinction. Information about the species is so limited and difficult to get. Fortunately, based on the preliminary study of the population shows that it can be found in Wilderness Zone, Bande Alit Resort, Meru Betiri National Park. The mapping of the species disperse in this location was also done.
PENGARUH PEMAPARAN SINAR GAMMA ISOTOP COBALT-60 DOSIS 0,25–1 kGy TERHADAP DAYA ANTAGONISTIK Trichoderma harzianum PADA Fusarium oxysporum
Authors: Wahyudi Priyo , Suwahyono Untung , Harsoyo Harsoyo , Mumpuni Aris , Wahyuningsih Dwi
Published: April 2017

Trichoderma harzianum is a well-known mycoparasitic fungus that has been used as biocontrol agent of many phytopathogenic fungi. One of the effort to improve the ability of wild strain of T. harzianum in its antagonistic activity is by exposed them in gamma ray iradiation. In this experiment wild strain of T. harzianum iradiated gamma ray of Cobalt-60 (0.25 kGy, 0.5 kGy, 0.75 kGy, and 1 kGy), then assess the effect of the iradiation on its growth, the antagonistic activity and chitinase activity toward Fusarium oxysporum. Results showed that iradiation of gamma ray 0.25–1 kGy has no effect on the growth of T. harzianum and its antagonistic activity, but it significantly influence the chitinase activity. Probably the fungi have repaired the damage of DNA caused by iradiation, so that the growth and even the enzymatic function has no longer affected.
EFEK ESTROGENIK EKSTRAK DAUN HANDEULEUM {Graptophyllum pictum (L.) Griff)} PADA HISTOLOGI UTERUS MENCIT BETINA OVARIEKTOMI
Authors: Suhargo Listijani
Published: April 2017

Estrogenic effects of handeuleum leaf {Graptophyllum pictum (L.)Griff)} were showed by uterus histology examinations of ovariectomy female mice. Female mice aged 3 months, were treated by gavage with handeuleum leaf extracts, ethinyl estradiol, aqua dest, and olive oil. The results showed that the treatment of handeuleum leaf extracts (with concentration 0.5 mg/0.05 ml olive oil) could increase uterus diameter, mucous layer thickness, lumen epithelial cell height, and glandular epithelial cell height if it was compared with aquadest treatments. Compared with ethinyl estradiol as standard estrogen, handeuleum leaf extracts have lower effects than ethinyl estradiol.
STUDI KANDUNGAN LOGAM BERAT DALAM DAGING IKAN DARI TAMBAK YANG DEKAT DAN YANG JAUH DARI DAERAH INDUSTRI
Authors: Kohar Indrajati , Budiono Ryanto , Indriany Diana , Wilujeng Nanik S.
Published: April 2017

Water pollution lately is often becomes an important topic due to its correlation with health. Heavy metals that accumulated in human body mostly come from food. One kind of food that used to be contaminated by heavy metals is fish. Therefore it is interesting to study the heavy metals content in fish, whether it comes from industrial contaminated water. Study on the content of heavy metals Cr, Cu, Pb and Zn in ponds near and far from industrial areas has been conducted. The chosen areas were one from Sidoarjo area and one from Babat area, both are located in East Java. Samples were the meat of fish called tilapia (from Sidoarjo area), and milkfish and goldfish (from Babat area), also the water and mud from the ponds. Analytical method was wet destruction. Measurement of heavy metals content was done by Inductively Coupled Plasma Spectrometer (ICPS). In Babat area’s pond water was not found neither Cr, Cu, Pb nor Zn, while Zn was detected in Sidoarjo area’s, however, it was under the limit of detection. The mud of both ponds contained Cr, Cu, Pb and Zn. The goldfish contained only Cu and Zn, whereas the tilapia and milkfish contained Cu, Pb and Zn, however the metals content were not exceeded the maximum limit stated in the PPOM regulation No. 03725/B/SK/VII/89.
Medicago truncatula Rop GTPases expression in young nodules
Authors: Kusumawati Lucia , Mirabella Rossana , Geurts René
Published: April 2017

Small GTPases are monomeric guanine nucleotide binding proteins with molecular weight of 21 to 30 kD. Plants have plant-specific small GTPases, termed Rop (Rho GTPases from plants) which serve as signaling proteins in plant signal transduction. To have better understanding on nodulation process in legume, which Rop proteins that play a dominant role in nodule formation would be determined. Therefore, the expression of five Medicago truncatula Rop GTPase genes (MtRops) in young nodules were studied in this experiment. RT-PCR method was used to examine the expression of MtRops. The MtRops expression in inoculated M. truncatula plants were compared with those in uninoculated plants. Results of this studies showed that the expression of MtRop5 and MtRop6 were significantly induced in young nodules, it was suggesting that these Rops may have important role during nodule formation.
CACING PARASIT PADA Nycticebus coucang
Authors: Setyorini L.E. , Wirdateti Wirdateti
Published: April 2017

Seventeen slow loris, Nycticebus coucang were examined for the endoparasitic occurrence, four are alive and the others are cadavers. Eggs, embryonated eggs and larvae of Enterobius sp. (Nematode: Oxyuridae ) were found on the fecal examination. The adult and mature helminth of Syphacia sp. (Nematode: Oxyuridae) and Rictularia sp. (Nematode: Rictularidae), a species of the tape worm and species of the hook worm were found on the necropsy of thirteen cadavers.
PRODUKSI, ISOLASI DAN KARAKTERISASI ENZIM DEKSTRANASE dari Arthrobacter sp. B7
Authors: Baktir Afaf
Published: April 2017

Dextranase enzyme has been purified and characterized from Arthrobacter sp. B7. This enzyme was purified from the culture supernatant of Arthrobacter sp. B7 by procedure of native PAGE. The molecular size of the enzyme was estimated 72,5 kDa by SDS-PAGE. The N-terminal amino acid sequence of this enzyme determined using Edman degradation techniques were APVTADVGNLHT. SDS-PAGE and native-PAGE analysis revealed that the enzyme molecule consisted of one sub-unit.
PENENTUAN STABILITAS SEDIAAN KRIM TABIR SURYA DARI BAHAN EKSTRAK RIMPANG KENCUR (Kaempferia galanga L.)
Authors: Soeratri Widji , Ifansyah Noor , Fitrianingrum Diana
Published: April 2017

The objective of this research was to know the chemical stability of the etil para-metoksi sinamat (EPMS) formulated in sunscreen cream of oil in water base. The EPMS was isolated from Kaempferia galanga L. tuber pulvis with maceration-percolation method using ethanol 96 percent. The chemical stability of the EPMS formulated in sunscreen cream of the oil in water base was investigated by keeping the cream at room temperature. At appropriate interval the concentration of EPMS in cream was measured. The results showed that the EPMS formulated in sunscreen cream of oil in water base was unstable at room temperature for 70 days. The concentration of EPMS in cream significantly decreased after 70 days (p = 0,01) during keeping at room temperature with the coefficient degradation value (k)= 4.4.10-3/day.
PENINGKATAN KADAR PATCHOULI ALCOHOL PADA MINYAK NILAM (Pogostemon cablin Benth) DENGAN METODE DISTILASI FRAKSINASI VAKUM
Authors: Isfaroiny Rahma , Mitarlis Mitarlis
Published: April 2017

The quality of nilam (Pogostemon cablin Benth) oil depend on it patchouli alcohol value. At there time nilam farmers just has produced nilam oil with patchouli alcohol value about 26–28 percent. To increase this value, fractional vacuum distillation had been done in this study. Nilam oil has been isolated from drying leaf of nilam plant. Patchouli alcohol in this oil was analyzed by gas chromatography, next the oil was distillated by fractional vacuum and the patchouli alcohol was determined from each fraction. This study showed that fractional vacuum distillation of nilam oil gives a higher patchouli alcohol value. The increasing was 23.3387 percent; from 35.022 percent at nilam oil before fractination vacuum distillation to 58.3515 percent at the third fraction from fractional vacuum distillation. In fact, the fourth fraction had the highest patchouli alcohol, but it had dark colour so isn’t fill the standard quality.
KONSUMSI DAN EFISIENSI PAKAN PADA IKAN KERAPU BEBEK (Cromileptes altivelis) YANG DIPUASAKAN SECARA PERIODIK
Authors: Yuwono Edy , Sukardi Purnama , Sulistyo Isdy
Published: April 2017

The objective of the research was to find out the effect of repeating cycle of feed deprivation followed by fixed refeeding on the food consumption and feed efficiency of grouper Cromileptes altivelis. The experimental fish weight was 10.86 1.42 gram. The treatments included: (P1) group received repeating cycles of 1 day food deprivation followed by 2 days refeeding in 14 cycles, (P2) group received repeating cycle of 1 day food deprivation followed by 3 days refeeding in 10 cycles, (P3) group received repeating cycle of 1 day food deprivation followed by alternating 2 and 3 days refeeding in 12 cycles and, (K) group fed daily as control. Each treatment was assigned to 3 aquaria containing 150 l recirculated sea water according to completely randomized design. Twenty individuals were stocked in each aquaria. The fishes were fed with floating pellet twice a day at 09.00 and 15.00 to satiation for four weeks. Water quality was monitored. The results showed that repeating cycle of food deprivation followed by fixed refeeding affected food consumption and feed efficiency in grouper Cromileptes altivelis. Food consumption of fishes from treatment groups showed lower food consumption than control, but they showed higher daily food consumption and feed efficiency. This phenomenon could be applied in practical feeding management for application to mariculture.
PERBEDAAN KEBERHASILAN TINGKAT POLIPLOIDISASI IKAN MAS (Cyprinus carpio Linn.) MELALUI KEJUTAN PANAS
Authors: Mukti Akhmad Taufiq
Published: April 2017

The easiest, cheapest, simpliest and efficient polyploidization method was heat shock treatment. Each fish species has different tolerance either initial time, temperature or duration of heat shock. The aim of this study were to show and determine the heat shock effects on differentiation of hatching rate, abnormality, survival rate, and successful polyploidization of common carp. The method that used in this study was experiment. Treatment that used was heat shock 40° C during 1.5 minutes of common carp eggs to 3 and 29 minutes after fertilization. Ten replicates were carried out for each treatment. Parameters test were hatching rate, abnormality, survival rate and polyploidy induction by counting of nucleolus number. Data analysis that used was descriptive. The result of this study indicated that heat shock treatment influenced on hatching rate, abnormality, survival rate and polyploidy induction of the common carp polyploidization. Hatching rate of common carp, triploidy and tetraploidy were 22.63 ± 8.36 percent and 11.10 ± 8.60 percent, abnormality 13.81 ± 4.67 percent and 24.86 ± 8.37 percent, survival rate 52.64 ± 8.46 percent and 55.04 ± 8.15 percent and polyploidy induction 70 ± 7.07 percent and 60 ± 7.07 percent, respectively.
THE ISOLATION OF Aeromonas hydrophila AND Escherichia coli FROM LOU HAN Cichlasoma synspilum AND STUDIES OF THEIR HISTOPATHOLOGY CHANGES
Authors: Suprapto Hari , Sumartiwi L , Prawesthirini S. , Handiyatno D. , Azmijah Ajik
Published: April 2017

Penyebab kematian lou han Cichlasoma synspilum adalah Aeromonas hydrophila dan Escherichia coli, dengan ciri adanya pendarahan dan luka pada sirip dada. E. coli kemungkinan besar tidak menyebabkan kematian ikan sebab sampai sekarang belum pernah ada laporan bahwa bakteri tersebut menyebabkan sakit pada ikan di mana pun. Gejala klinis utama yang terlihat dari luar adalah pendarahan di sirip dada dan menimbulkan luka yang cukup dalam. Cairan ascites menyebabkan membengkaknya perut, pembengkakan liver, dan kosongnya usus dari ikan yang sakit. Gambaran histopatologi menunjukkan nekrosis dan adanya vacuole pada hepatocyte. Sedangkan pada usus adalah rusaknya mucosal ephitelium, lamina propia stratum compactum, stratum granulosum dan muscularis. Ginjal mengalami kerusakan berat, hampir semua organela ginjal berantakan. Ikan ini dipelihara di aquarium sehingga menurunnya kualitas air atau kontaminasi lewat pakan dan penanganan yang buruk diduga sebagai portal of entry dari penyakit.
PEMANFAATAN RIMPANG KUNIR PUTIH (Curcuma zedoaria) SEBAGAI PENGURANG KERUSAKAN STRUKTUR MIKROANATOMI HEPAR MENCIT AKIBAT ALKOHOL
Authors: Lisdiana Lisdiana
Published: April 2017

Curcuma zedoaria, familia Zingiberaceae, has been used for along time as an alternative healing. active compounds within it are coneol, comphene, borneol, camphor, zedoarin, resin, gum, and curcumin. Curcumin is an antioxidan that can slow cell damages caused by toxicant. The purpose of this research was to find out the role of Curcuma zedoaria’s rhizome for minimizing damage of microanatomical structure of liver caused by alcoholic toxicant. Samples in the research were 24 mice (Mus musculus) Swiss Webster. They were grouped randomly into 8 groups. Each group was treated as follow. Group A (control group) was given fresh water. Group B was given whisky. Group C was given 500 mg Curcuma zedoaria/kg body weight. Group D was given 750 mg Curcuma zedoaria/kg body weight. Group E was given Curcuma zedoaria/kg body weight. Group F was given whisky and, 2 hours later, 500 mg Curcuma zedoaria/kg body weight. Group G was given whisky and, 2 hours later, 750 mg Curcuma zedoaria/kg body weight. The last group was given whisky and, 2 hours later, 1000 mg Curcuma zedoaria/kg body weight. The treatment was carried out during 8 days. A microanatomy slide of liver was made by using parafin method and Hematoxylin-Eosin dying. Data was analyzed by using descriptive method, comparing between the control group and the experiment groups. The result of the research showed that the powder of the Curcuma zedoaria’s rhizome has been useful in minimizing damage of hepar microanatomy structure of the mice affected by alcohol. The effective dosage was 1000 mg/kg body weight.
POTENSI KAPANG PELAPUK PUTIH Phanerochaete chrysosporium DALAM PENGOLAHAN LIMBAH INDUSTRI TEKSTIL
Authors: Trihadiningrum Yulinah
Published: April 2017

Phanerochaete chrysosporium was known as white rot mold which could biodegrade persistent organic pollutants. In this research the ability of the mold in biodegrading textile dye direct orange S and rhemazol yellow, which contained naphtol functional group, was studied. This research included characterization of the waste water and determination of optimum concentration of the waste water for biodegradation. The optimum concentration for biodegradation was determined by measuring the radial growth of the mold in agar medium containing various concentrations of textile dye. The final stage was the application of the mold for biodegrading the textile dye in aerobic batch reactor using the selected concentration. This research showed that P. chrysosporium could grow satisfactorily in minimum medium containing synthetic textile wastewater in various concentrations. The highest colour removal efficiency of 93 percent was achieved in 3 days in the waste water with highest color intensity (absorbance value l520 = 4.00), and 48 percent in the waste water with absorbance value l520 = 1.22. COD removal efficiency of 87 percent was achieved within 12 days in the wastewater of 30 percent concentration, and 83 percent in the waste water of 100 percent concentration. The pH values decreased to 3.23 in the waste water with lower dye concentration and to 3.42 in the higher color intensity, from the initial values of 5.0-6.0. This research concluded that P. chrysosporium was capable to biodegrade naphtol textile dye with reasonably high efficiency.
SUKSESI SEKUNDER PADA LAHAN TAMBAK TERLANTAR DI KAWASAN HUTAN MANGROVE SEGARA ANAKAN CILACAP JAWA TENGAH
Authors: Kartijono Nugroho Edi
Published: April 2017

The study of secondary succession was carried out on Segara Anakan mangrove forest, since the secondary succession has been the significant problem in the mangrove forest Segara Anakan as the impact of illegal cutting and the neglected fishpond activities. Side-by-side comparisons method and quadrat sampling method were employed in this study. There were 3 areas of abandoned fishpond with different levels of succession. The sample stands were determinated subyectievely and the quadrat sample were put at random. Ordination method was used to see the correlation between vegetation pattern using environment parameters observed. The result of this study indicated that the important value on the stratum of mangrove herb was succession process. The ordination pattern of mangrove was not related to environment factors. In general, some spesies: Avicennia alba, Acanthus ilicifolius, Paspalum sp., Fimbristylis alboviridis, Bulbostylis puberula had the important role in the process of secondary succession.
PENGARUH PEMBERIAN PERASAN Sechium edule (Jacq.) Swartz TERHADAP KADAR KOLESTEROL TOTAL DAN TRIGLISERIDA SERA MENCIT QUECKERBUS
Authors: Dyatmiko Wahjo , Maat Suprapto , Kusumawati Idha , Santoso Anang Teguh Eko
Published: April 2017

This research was an effort to reveal the effect of fruit juice of labu siam (Sechium edule (Jacq.) Swartz) on total cholesterol and total glyceride of mice serum and also to reveal the secondary metabolites content of the juice. In this research, four groups of mice each consist of 8 was used. First group ws given normal food, the second group was given hyperlipidemic inducer. The third group was given hyperlipidemic inducer and fruit juice at dosage 0.546 mg/kg b.w. The third group was given hyperlipidemic inducer and fruit juice at dosage 1.092 mg/g b.w. The exogenic and endogenic hyperlipidemic inducers used were cholesterol suspension and propyl thiouracyl 0.01 percent, espectively. Both the inducer and the juice were given every day orally for three weeks. At the last day, the mice were fasted for 12-24 hours and the blood was collected intracardially. Assay of cholesterol and triglyceride in the sera was carried out using the reagent kits. Statistical analysis shows that the administration of the fruit juice of ‘labu siam’ at dosage 0.546 mg/g and 1.092 mg/g b.w. can decrease the triglyceride concentration in the sera. The dose 0.546 mg/g decreases the total cholesterol concentration, but the dose 1.092 mg/g did not show the sera cholesterol decreasing activity. Phytochemical screening for the constituents of the juice indicated that the juice contains saponin glycosides.
LAJU PENINGKATAN KONSENTRASI TIMBAL (Pb) DALAM DARAH HEWAN UJI MENCIT (Mus musculus) DI BENGKEL OTOMOTIF
Authors: Razif Mohammad , Sukatma Sukatma
Published: April 2017

This research was conducted in automotive workshop of VEDC Malang and the samples were taken at the automotive machine and automotive electric workshops. The parameters which examined were the Pb concentration in the mouse blood (Mus musculus) in 2 and 3 months years old. The measurement was run for 5 times every 10 days, and the result was mean value of the increasing rate of Pb concentration in the mouse blood. The aim of this research was to determine the increasing rate of Pb concentration in the mouse blood which has the relation with different working area, different age, and also duration of exposure. Based on the data of laboratory test, research continued by T-test analysis, Simple regression test, and correlation, and also General Linear Model. By doing statistic analysis with the different working area, duration of explanation, and different age. Result of this research showed that there were the differences of rapid increasing of Pb concentration in both workshop. Generally the age which influence so much for that (in the mouse body) and have the relation with the body stamina. If the mouse body absorb amount of Pb constantly, so the Pb concentration in it will increase more and more and it cause the decreasing of the body height.
UJI EFEK ANTIINFLAMASI EKSTRAK HERBA SURUHAN (Peperomia pellucida L. KUNTH) PADA TIKUS PUTIH JANTAN
Authors: Wijaya Sumi , S.W Monica
Published: April 2017

Peperomia pellucida L. Kunth herbs (Herba Suruhan) is one of the alternative for inflamation therapy. In this study, albino rats were devided into seven groups. The first three groups was administered orally the extract of Suruhan herbs 1500 mg/Kg body weight, 2000 mg/Kg body weight and 2500 mg/Kg body weight. The next three groups were given Indomethacine solutions with the doses of 13.5 mg/Kg body weight, 15.8 mg/Kg body weight and 15 mg/Kg body weight, and the last groups (control) were given 1 percent of PGA suspension alone. Fifty microliter of Carrageen solution of 1.5 percent was injected subcutaneously to all rats in those group. The volume of the foot paw of albino male rat injected with carrageen, was measured and recorded for 4 hours. The number of leucocyte and the number of differential count were observed every two hour, namely at the fourth, sixth and eight hours following carrageen injection. There were significant differences in the antiinflamation effect using plethysmometer and the number of leucocyte counts between control and all the treatment group. For the differential count, not all the results showed significant differences.
Injuries in recreational curling include head injuries and may be prevented by using proper footwear
Authors: Ting DK , Brison RJ
Published: April 2017

Introduction

Our study examines a recreational curling population to describe patterns
of injury occurrence, estimate risk of injury and to gauge attitudes towards equipmentbased
prevention strategies.

Methods

In a retrospective case series, we queried the Canadian Hospitals Injury
Reporting and Prevention Program (CHIRPP), a national injury surveillance database,
for curling injuries entered between 1993 and 2011. Kingston General Hospital and Hotel
Dieu Hospital provide the two Kingston, Ontario, sites for emergency department (ED)
care and participate in CHIRPP. Each retrieved entry underwent a chart review. A
secondary survey was mailed to select individuals who had experienced curling injuries
to solicit details on their injury and attitudes towards equipment to prevent injury. We
used descriptive statistics for rates and proportions.

Results

Over 90% of acute curling injuries resulted from a fall, and 31.7% were head
impacts. We found that acute injuries requiring ED presentation occur at a rate of
approximately 0.17 per 1000 athlete-exposures (95% CI: 0.12–0.22). The secondary
survey was completed by 54% of potential respondents. Of survey respondents, 41.3%
attributed their fall to a lack of proper footwear and 73.5% of respondents agreed with
mandatory sport-specific footwear as a prevention strategy, but only 8% agreed with
mandatory helmet wear.

Conclusion

Although curling injuries requiring medical care are not common, head injuries
make up a large proportion. Mandated use of appropriate footwear appears to be the most
effective prevention strategy, as well as the measure deemed most acceptable by players.
Customer Relations Management sebagai Salah Satu Upaya Public Relations Perusahaan Jasa Perbankan Menciptakan Good Image
Authors: Mahanani Prima Ayu Rizqi
Published: April 2017

This article deals with marketing trends in the 21st century is shifting from a transactional approach to the relational approach with focus on the fulfillment of needs, satisfaction, and pleasure affect business banking customers are very dependent on the customer. Map business services banking services fickle demands creativity public relations in providing the best service to its customers get a good image. Service concept using the service paradigm leading to cultivate the power of the customer based on the customer satisfaction-oriented, widely used by business banking services at this time. Paradigms that focus on how to provide services to the customer so that the customer was satisfied, beyond what can be given to something that is important and not to be underestimated. Skills to understand and fulfill customer expectations should be every company’s business philosophy of banking services and customer relationship management is a strategy
Model Komunikasi Kelompok Tentang Makna Pernikahan Antargenerasi di Kalangan Kelas Menengah Jakarta
Authors: Kholisoh Nur , Primayanti Primayanti
Published: April 2017

Nowadays the meaning of marriage has changed as found in various phenomena of exiting from state regulations and laws, religion, customs and norms in society. The research aims at finding interaction model and group communication leading to changes of meaning of marriage among inter-generation of middle-class groups in in DKI Jakarta. This research refers to Alfred Schutz teories of symbolic interactionism, group-communication, and social phenomenology. Qualitative research method is used to analyze data completed by depth interview and FGD as data collection technique. The results shows that interaction model on mariage meaning is influenced by the way people grow, interact, and communicate within their group. The meaning of marriage symbols which is exchanged in the interaction of a group has changed from one generation to the next generation. Marriage is perceived as destiny, the unity between man and woman as a way of preserving human beings, justifying sexual intercourse and holding religious order. The research also fond contradictive phenomenon of marriage meaning between marriage meaning with the sacred law of marriage, whether it is in state regulations, customs and norms of society, such as same-sex marriage phenomenon (gay/lesbian), swinging behavior, samen leven, and free sex.
Literasi Digital pada Perempuan Pelaku Usaha Produktif di Daerah Istimewa Yogyakarta
Authors: Widyastuti Dhyah Ayu Retno , Nuswantoro Ranggabumi , Sidhi Thomas Adi Purnomo
Published: April 2017

This research aims at identifying the reality of women activities in using digital media. This research was conducted at Usaha Mikro Kecil dan Menengah (UMKM) in Daerah Istimewa Yogyakarta. Using qualitative research approach to observe information among informants, this resaerch found that women have limited access to information and communication technology. Women used digital media to get information to support their activities. They used mobile phone and personal computer to access the internet. The use of mobile phone was more than personal computer. Based on the result, this research recommends that digital literacy played important role to sustain women activities. The process of adoption of innovative technologies enhances women capability to improve UMKM activities. In addition, internet access enhances women’s economic sustainability.