bluuebunny/crossref_metadata_2025 huggingface dataset:
Business IT Alignment and Technology Adoption; The Case of RFID in the Logistics Domain
Authors: van de Wijngaert Lidwien , Versendaal Johan , Matla René
Published: November 2020

Radio Frequency Identification (RFID) is increasingly applied in the logistics domain. As with other emerging technologies it is complex whether, when and how an organization should invest in RFID. In this paper the decision to adopt RFID in the logistic domain is explored. The authors take a business/IT-alignment approach and investigate the relation between business/IT-alignment principles and preference for RFID applications. A comparison of thirteen organizations that operate one or more warehouses shows that managers and decision makers in companies are not necessarily in sync with the business/IT-alignment principle which implies to further mature in the areas/domains that are least developed. Organizations were more prone to invest in business/IT areas that are already well developed. We conclude that other factors influence decisions to apply this type of new technology. Yet, the framework offers the opportunity to analyze and prepare a technology decision; it provides insight in and arguments for possible RFID adoption.
Enhancing Information Flow in a Retail Supply Chain Using RFID and the EPC Network: A Proof-of-Concept Approach
Authors: Wamba Samuel Fosso , Boeck Harold
Published: November 2020

RFID technology and the Electronic Product Code (EPC) network have attracted considerable interest from businesses and academics in recent years. The interest is even stronger in the retail industry where firms such as Best Buy, Wal-Mart, Tesco, Target and Metro AG are capitalizing on the potential of these technologies. Based on a field study conducted in a three-layer retail supply chain, this paper tests several scenarios integrating Radio Frequency Identification (RFID) technology and the EPC network and evaluates, in a laboratory setting, their potential as enablers of information flow within a retail supply chain. Using an “openloop” adoption strategy, our preliminary results indicate that RFID technology and the EPC network (i) hold some potential that can be grasped through Business Process Management (BPM), (ii) enable the synchronization of information flow with product flow in a given supply chain, and thus, (iii) provide a better level of information integration between supply chain members. The results suggest that these “new waves” of information technology (IT) could in fact provide end-to-end information flow between supply chain members.
Green Transition of Cultivated Land Use in the Yellow River Basin: A Perspective of Green Utilization Efficiency Evaluation
Authors: Lu Xiao , Qu Yi , Sun Piling , Yu Wei , Peng Wenlong
Published: November 2020

Exploring the green transition of cultivated land use from the perspective of green utilization efficiency evaluation has become an important content of deepening the study of cultivated land use transition, which is of great significance to promote food security and ecological civilization construction. At present, there are few studies on the green utilization efficiency of cultivated land (GUECL), which covers the comprehensive benefits of economy, ecology and society, combined with the requirements of ecological civilization and green development. Taking 65 cities (regions and autonomous prefectures) of the Yellow River Basin as the basic evaluation unit, the GUECL of the Yellow River Basin is evaluated with a Super-SBM model. In general, the GUECL of the Yellow River Basin was not high at four time points of 2000, 2006, 2012 and 2018, which presents a trend of “rising first and then falling”. Analyzing its temporal and spatial evolution pattern, the GUECL in the upper, middle and lower reaches presented an order of the upper reaches area > the lower reaches area > the middle reaches area; and the spatial variation trend showed a decrease from west to east, and a U-shaped change in the south-north direction. Using spatial correlation analysis, except for the year 2000, the GUECL in the Yellow River Basin presents a general distribution characteristic of spatial agglomeration, which is positively correlated in 2006, 2012 and 2018. The change of spatio-temporal pattern is the result of internal and external factors. The former mainly displays in the main characteristics of farmers, family characteristics and farmers’ cognition, while the latter is reflected in natural, social and policy factors.
Establishment of Permafrost Thermal Monitoring Sites in East Siberia
Authors: Konstantinov Pavel , Zhelezniak Mikhail , Basharin Nikolay , Misailov Ivan , Andreeva Varvara
Published: November 2020

Permafrost lies close to the surface of the day, therefore, it is able to quickly respond to modern climatic changes. Under these conditions, the goal of understanding the evolution of permafrost in the near future requires monitoring studies of the current state of permafrost and, first of all, its thermal conditions. In this work, based on the experience of many years of research at the Melnikov Permafrost Institute of Siberian Branch of the Russian Academy of Science (MPI SB RAS), methodological and technical issues of equipping experimental sites for monitoring the thermal state of permafrost in Eastern Siberia are considered. It is demonstrated that the reliability of permafrost thermal monitoring depends not only on measurement devices used but also on proper borehole system design and adequate choice of a method for active-layer thickness measurement depending on soil composition and properties. The use of protective tubes significantly lengthens the life of sensors in soils. A method of protecting the loggers from surface waters is recommended.
Comparative Proteomic Analysis of Walnut (Juglans regia L.) Pellicle Tissues Reveals the Regulation of Nut Quality Attributes
Authors: Zaini Paulo A. , Feinberg Noah G. , Grilo Filipa S. , Saxe Houston J. , Salemi Michelle R. , Phinney Brett S. , Crisosto Carlos H. , Dandekar Abhaya M.
Published: November 2020

Walnuts (Juglans regia L.) are a valuable dietary source of polyphenols and lipids, with increasing worldwide consumption. California is a major producer, with ’Chandler’ and ’Tulare’ among the cultivars more widely grown. ’Chandler’ produces kernels with extra light color at a higher frequency than other cultivars, gaining preference by growers and consumers. Here we performed a deep comparative proteome analysis of kernel pellicle tissue from these two valued genotypes at three harvest maturities, detecting a total of 4937 J. regia proteins. Late and early maturity stages were compared for each cultivar, revealing many developmental responses common or specific for each cultivar. Top protein biomarkers for each developmental stage were also selected based on larger fold-change differences and lower variance among replicates, including proteins for biosynthesis of lipids and phenols, defense-related proteins and desiccation stress-related proteins. Comparison between the genotypes also revealed the common and specific protein repertoires, totaling 321 pellicle proteins with differential abundance at harvest stage. The proteomics data provides clues on antioxidant, secondary, and hormonal metabolism that could be involved in the loss of quality in the pellicles during processing for commercialization.
Effect of Initial State and Deformation Conditions on the Hot Deformation Behavior of M50NiL Steel
Authors: Zhang Yan , Yang Ming , Long Shaolei , Li Bo , Liang Yilong , Ma Shaowei
Published: November 2020

M50NiL steel, which belongs to a new generation of case-hardening steels used in aerospace bearing applications, is used mainly for the manufacturing of aerospace transmission components that operate under high temperatures. In this study, the effects of the hot deformation parameters and the initial microstructure on the hot deformation behavior of M50NiL steel were investigated through Gleeble-3500 isothermal hot compression tests. The experimental results demonstrated that the critical stain of dynamic recrystallization and the deformation activation energy of the coarse-grained samples were higher than those of the fine-grained samples. This is attributed to the difficulty of deformation and the dynamic recrystallization behavior of coarse-grained samples. Moreover, fine-grained samples contain a large number of dispersed phases, which can pin the grain boundaries and inhibit the growth of recrystallized grains. Such phenomena are beneficial for obtaining finer and more uniform microstructures in M50NiL steel. The experimental results can provide a useful reference for preparing M50NiL steel with excellent mechanical properties.
Realization of a Dental Framework by 3D Printing in Material Cobalt-Chromium with Superior Precision and Fitting Accuracy
Authors: Edelmann André , Riedel Lisa , Hellmann Ralf
Published: November 2020

We report on the generation of a cobalt-chromium dental framework with superior precision and fitting accuracy using selective laser melting. The objective of this study is the reduction of surface roughness and the possibility to manufacture a dental framework with high precision for passive fit with attachments, in particular a round tack. After selective laser melting, the dental framework is thermally post processed at 750 °C, shot-blasted with glass and highly polished. Nominal to actual 3D form deviation is analyzed by stripe light projection, revealing deviations being less than 250 μm, i.e., warpage is as low as to permit dental application and accurate passive fit. In particular, the critical area of the dental framework, the fixture to the implant (overdenture) shows negligible deviations. This superior fitting accuracy is confirmed by joining the bar with a testing stylus.
Characteristics and Transformation Mechanism of Nonmetallic Inclusions in 304 Stainless Steel during Heat Treatment at 1250 °C
Authors: Yang Wen-Sheng , Liu Shuai , Han Shao-Wei , Wang Jia-Wei , Guo Jing , Yan Yan , Guo Han-Jie
Published: November 2020

Evolutions of two typical types of nonmetallic inclusions, i.e., inclusions based on CaO-SiO2-Al2O3 and MnO-SiO2-Al2O3 of 304 stainless steel were investigated in laboratory-scale experiments under isothermal heat treatment at 1250 °C for 0, 30, 60 and 120 min. Results show inclusion population density increases at the first stage and then decreases while their average size decreases and then increases. Moreover, almost no Cr2O3 content within the inclusion before the heat treatment, but Cr2O3 content increases gradually along with increasing heat treatment time. Furthermore, the increasing of Cr2O3 content in the inclusions would increase their melting points and reduce their plasticities. The experimental results and thermodynamic analysis indicate that there are three steps for inclusion evolution during the heat treatment process, in which Ostwald ripening plays an important role in inclusion evolution, i.e., inclusions grow by absorbing the newly formed small-size MnO-Cr2O3 inclusions.
Flexural Response of Degraded Polyurethane Foam Core Sandwich Beam with Initial Crack between Facesheet and Core
Authors: Dhaliwal Gurpinder Singh , Newaz Golam M.
Published: November 2020

Structural systems developed from novel materials that are more durable and less prone to maintenance during the service lifetime are in great demand. Due to many advantages such as being lightweight as well as having high strength, corrosion resistance, and durability, the sandwich composites structures, in particular, have attracted attention as favorable materials for speedy and durable structural constructions. In the present research, an experimental investigation is carried out to investigate the flexural response of sandwich beams with a pre-cracked core-upper facesheet interface located at one end of the beam. During the development of the sandwich beams, an initial pre-cracked debond was created between the core and facesheet by placing a Teflon sheet at the interface. Both three-point and four-point flexural tests were conducted to characterize the flexural behavior of the sandwich beams. The effects of the loading rate, core thickness, and placement of the initial interfacial crack under a compressive or tensile stress state on the response and failure mechanism of Carbon Fiber-Reinforced Polymer (CFRP)/Polyurethane (PU) foam sandwich beams were investigated. It was found that the crack tip of the initial debonding between the upper facesheet and the core served as a damage initiation trigger followed by the fracture failure of the core due to the growth of the initial crack into the core in an out-of-plane mode. Finally, this leads to facesheet damage and rupture under flexural loadings. An increase in the core thickness resulted in a higher peak load, but the failure of the sandwich beam was observed to occur at significantly lower displacement values. It was found that the behavior of sandwich beams with higher core thickness was loading rate-sensitive, resulting in stiffer response as the loading rate was increased from 0.05 to 1.5 mm/s. This change in stiffness (10–15%) could be related to the squeezing of all pore space, resulting in the collapse of cell walls and thereby making the cell behave as a solid material. As a result, the occurrence of the densification phase in thick core beams occurs at a faster rate, which in turn makes the thick cored sandwich beams exhibit loading rate-sensitive behavior.
The Stability of Two-Community Replicator Dynamics with Discrete Multi-Delays
Authors: Pi Jinxiu , Yang Hui , Shu Yadong , Zhong Chongyi , Yang Guanghui
Published: November 2020

This article investigates the stability of evolutionarily stable strategy in replicator dynamics of two-community with multi-delays. In the real environment, players interact simultaneously while the return of their choices may not be observed immediately, which implies one or more time-delays exists. In addition to using the method of classic characteristic equations, we also apply linear matrix inequality (i.e., LMI) to discuss the stability of the mixed evolutionarily stable strategy in replicator dynamics of two-community with multi-delays. We derive a delay-dependent stability and a delay-independent stability sufficient conditions of the evolutionarily stable strategy in the two-community replicator dynamics with two delays, and manage to extend the sufficient condition to n time delays. Lastly, numerical trials of the Hawk–Dove game are given to verify the effectiveness of the theoretical consequences.
Ordinary Differential Equation with Left and Right Fractional Derivatives and Modeling of Oscillatory Systems
Authors: Eneeva Liana , Pskhu Arsen , Rekhviashvili Sergo
Published: November 2020

We consider the principle of least action in the context of fractional calculus. Namely, we derive the fractional Euler–Lagrange equation and the general equation of motion with the composition of the left and right fractional derivatives defined on infinite intervals. In addition, we construct an explicit representation of solutions to a model fractional oscillator equation containing the left and right Gerasimov–Caputo fractional derivatives with origins at plus and minus infinity. We derive a representation for the composition of the left and right derivatives with origins at plus and minus infinity in terms of the Riesz potential, and introduce special functions with which we give solutions to the model fractional oscillator equation with a complex coefficient. This approach can be useful for describing dissipative dynamical systems with the property of heredity.
Some Remarks on Fuzzy sb-Metric Spaces
Authors: Öner Tarkan , Šostak Alexander
Published: November 2020

Fuzzy strong b-metrics called here by fuzzy sb-metrics, were introduced recently as a fuzzy version of strong b-metrics. It was shown that open balls in fuzzy sb-metric spaces are open in the induced topology (as different from the case of fuzzy b-metric spaces) and thanks to this fact fuzzy sb-metrics have many useful properties common with fuzzy metric spaces which generally may fail to be in the case of fuzzy b-metric spaces. In the present paper, we go further in the research of fuzzy sb-metric spaces. It is shown that the class of fuzzy sb-metric spaces lies strictly between the classes of fuzzy metric and fuzzy b-metric spaces. We prove that the topology induced by a fuzzy sb-metric is metrizable. A characterization of completeness in terms of diameter zero sets in these structures is given. We investigate products and coproducts in the naturally defined category of fuzzy sb-metric spaces.
Enhancing Computational Thinking through Interdisciplinary STEAM Activities Using Tablets
Authors: Valovičová Ľubomíra , Ondruška Ján , Zelenický Ľubomír , Chytrý Vlastimil , Medová Janka
Published: November 2020

Computational thinking is a highly appreciated skill by mathematicians. It was forecasted that, in the next few years, half of the jobs in science, mathematics, technology and engineering (abbreviated as STEM, including arts as STEAM) will use some kind of computation. It is therefore necessary to enhance the learning of mathematics by collaborative problem-solving activities focused on both learning mathematics and developing computational thinking. The problems in science offer a reasonable context in which to investigate the common overarching concepts (e.g., measuring the length). An interdisciplinary STEAM collaborative problem-solving activity was designed and piloted with 27 lower secondary students aged 13.07 ± 1.21 years. Different levels of willingness to use the technology were observed and the factors influencing it were identified. We found that strong background knowledge implies high demands when controlling the used device. On the other hand, when a nice and user-friendly application was used, students did not need to perceive any control over it. After the intervention, the students’ views on the tablet changed and they reported more STEAM-related functions of the device.
Formulation of Orally Disintegrating Films as an Amorphous Solid Solution of a Poorly Water-Soluble Drug
Authors: Panraksa Pattaraporn , Tipduangta Pratchaya , Jantanasakulwong Kittisak , Jantrawut Pensak
Published: November 2020

The objective of the present study was to develop an orally disintegrating film (ODF) for a poorly water-soluble drug, phenytoin (PHT), using the cosolvent solubilization technique to achieve the amorphization of the drug, followed by the preparation of ODFs. Eleven formulations were prepared with different polymers, such as polyvinyl alcohol (PVA) and high methoxyl pectin (HMP) by the solvent casting method. The prepared films were subjected to characterization for weight variations, thickness, surface pH, disintegration time and mechanical strength properties. Then, differential scanning calorimetry, X-ray diffraction analysis and the drug release patterns of the selected films were evaluated. Among the prepared formulations, the formulation composed of 1% w/w of PVA, 0.04% w/w of sodium starch glycolate with polyethylene glycol 400, glycerin and water as cosolvents (PVA-S4) showed promising results. The physical appearance and mechanical strength properties were found to be good. The PVA-S4 film was clear and colorless with a smooth surface. The surface pH was found to be around 7.47 and the in vitro disintegration time was around 1.44 min. The drug content of the PVA-S4 film was 100.27%. X-ray diffractometry and thermal analysis confirmed the transition of phenytoin in the PVA-S4 film into a partially amorphous state during film preparation using the cosolvent solubilization approach. The resulting PVA-S4 film showed a higher dissolution rate in comparison to the film without a cosolvent. Overall, this study indicated the influence of cosolvents on enhancing the solubility of a poorly water-soluble drug and its film dissolution.
Computer-Aided Engineering Environment for Designing Tailored Forming Components
Authors: Brockmöller Tim , Siqueira Renan , Gembarski Paul C. , Mozgova Iryna , Lachmayer Roland
Published: November 2020

The use of multi-material forming components makes it possible to produce components adapted to the respective requirements, which have advantages over mono-material components. The necessary consideration of an additional material increases the possible degrees of freedom in product and manufacturing process development. As a result, development becomes more complex and special expert knowledge is required. To counteract this, computer-aided engineering environments with knowledge-based tools are increasingly used. This article describes a computer-aided engineering environment (CAEE) that can be used to design hybrid forming components that are produced by tailored forming, a process chain developed in the Collaborative Research Center (CRC) 1153. The CAEE consists of a knowledge base, in which the knowledge necessary for the design of tailored forming parts, including manufacturer restrictions, is stored and made available. For the generation of rough and detailed design and for elaboration the following methods are used. The topology optimization method, Interfacial Zone Evolutionary Optimization (IZEO), which determines the material distribution. The design of optimized joining zone geometries, by robust design. The elaboration of the components by means of highly flexible computer-aided design (CAD) models, which are built according to the generative parametric design approach (GPDA).
Effect of Surface Modification of a Titanium Alloy by Copper Ions on the Structure and Properties of the Substrate-Coating Composition
Authors: Fedorischeva Marina , Kalashnikov Mark , Bozhko Irina , Perevalova Olga , Sergeev Victor
Published: November 2020

To improve the strength properties, adhesion, and the thermal cycling resistance of ceramic coatings, the titanium alloy surface was modified with copper ions under different processing times. It is found that at the maximum processing time, the thickness of the alloyed layer reaches 12 μm. It is shown that the modified layer has a multiphase structure in addition to the main α and β–titanium phases with the intermetallic compounds of the Ti-Cu system. The parameters of the fine structure of the material are investigated by the X-ray diffraction analysis. It has been found that when the surface of the titanium alloy is modified, depletion occurs in the main alloying elements, such as aluminum and vanadium, the crystal lattice parameter increases, the root-mean-square (rms) displacements of the atoms decrease, and the macrostresses of compression arise. A multilevel micro- and nanoporous nanocrystalline structure occurs, which leads to an increase in the adhesion and the thermal cyclic resistance of the ceramic coating based on Si-Al-N.
Metabolomic Analysis of Plasma from GABAB(1) Knock-Out Mice Reveals Decreased Levels of Elaidic Trans-Fatty Acid
Authors: Fattuoni Claudia , Barberini Luigi , Noto Antonio , Follesa Paolo
Published: November 2020

Mice lacking the GABAB(1) subunit of gamma-aminobutyric acid (GABA) type B receptors exhibit spontaneous seizures, hyperalgesia, hyperlocomotor activity, and memory impairment. Although mice lacking the GABAB(1) subunit are viable, they are sterile, and to generate knockout (KO) mice, it is necessary to cross heterozygous (HZ) mice. The aim of our study was to detect the metabolic differences between the three genotypes of GABAB(1) KO mice in order to further characterize this experimental animal model. Plasma samples were collected from wild-type (WT), HZ, and KO mice. Samples were analyzed by means of a gas chromatography-mass spectrometry (GC-MS) platform. Univariate t-test, and partial least square discriminant analysis (PLS-DA) were performed to compare the metabolic pattern of different genotypes. The metabolomic analysis highlighted differences between the three genotypes and identified some metabolites less abundant in KO mice, namely elaidic acid and other fatty acids, and chiro-inositol.
Good Cop, Bad Cop: The Opposing Effects of Macrophage Activation State on Maintaining or Damaging Functional β-Cell Mass
Authors: Jensen Daelin M. , Hendricks Kyle V. , Mason Austin T. , Tessem Jeffery S.
Published: November 2020

Loss of functional β-cell mass is a hallmark of Type 1 and Type 2 Diabetes. Macrophages play an integral role in the maintenance or destruction of pancreatic β-cells. The effect of the macrophage β-cell interaction is dependent on the activation state of the macrophage. Macrophages can be activated across a spectrum, from pro-inflammatory to anti-inflammatory and tissue remodeling. The factors secreted by these differentially activated macrophages and their effect on β-cells define the effect on functional β-cell mass. In this review, the spectrum of macrophage activation is discussed, as are the positive and negative effects on β-cell survival, expansion, and function as well as the defined factors released from macrophages that impinge on functional β-cell mass.
Dynamic Analysis of an Underwater Cable-Driven Manipulator with a Fluid-Power Buoyancy Regulation System
Authors: Wang Tong , You Zihao , Song Wei , Zhu Shiqiang
Published: November 2020

This article presents an underwater cable-driven manipulator (UCDM) with a buoyancy regulation system (BRS), which is controlled by a fluid-power system. The manipulator consists of five sections, and each section is embedded with a buoyancy adjustment unit. By regulating buoyancy at each section, the static and dynamic states of the manipulator will be changed, promising a new operating mode of an underwater manipulator driven by buoyancy. In this article, a dynamic model of the manipulator is established by the Newton-Euler equation, considering cable tension, inter-joint force, buoyancy, water resistance and other variables. With a numerical method, the dynamic model is solved and the values of cable tension are obtained, which are used to evaluate the buoyancy-driven operating mode of underwater manipulator. This research will be useful for manipulator operating in fluid environments, such as underwater manipulator in the ocean, micro-manipulator in a blood vessel, and so on.
Fiber Raman Amplifiers and Fiber Raman Lasers
Authors: Sirleto Luigi
Published: November 2020

Stimulated Raman scattering (SRS) is a nonlinear optical effect, observed for the first time in 1962, which lies at the heart of fiber Raman amplifiers and fiber Raman lasers [...]
A Review of Asbestos Bioweathering by Siderophore-Producing Pseudomonas: A Potential Strategy of Bioremediation
Authors: David Sébastien R. , Geoffroy Valérie A.
Published: November 2020

Asbestos, silicate minerals present in soil and used for building constructions for many years, are highly toxic due primarily to the presence of high concentrations of the transition metal iron. Microbial weathering of asbestos occurs through various alteration mechanisms. Siderophores, complex agents specialized in metal chelation, are common mechanisms described in mineral alteration. Solubilized metals from the fiber can serve as micronutrients for telluric microorganisms. The review focuses on the bioweathering of asbestos fibers, found in soil or manufactured by humans with gypsum (asbestos flocking) or cement, by siderophore-producing Pseudomonas. A better understanding of the interactions between asbestos and bacteria will give a perspective of a detoxification process inhibiting asbestos toxicity.
Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis
Authors: Bhavanam Sudha , Freedman Stephen , Lee Bonita , Zhuo Ran , Qiu Yuanyuan , Chui Linda , Xie Jianling , Ali Samina , Vanderkooi Otto , Pang Xiaoli ,   on behalf of the Alberta Provincial Pediatric Enteric Infection Team (APPETITE)
Published: November 2020

Norovirus is a major pathogen identified in children with acute gastroenteritis (AGE), little is known about the strain’s diversity and their clinical severity. Stool and/or rectal swabs were collected from children ≤18 years of age recruited at emergency departments (ED), and a provincial nursing advice phone line due to AGE symptoms in the province of Alberta, Canada between December 2014 and August 2018. Specimens were tested using a reverse transcription real time PCR and genotyped by Sanger sequencing. The Modified Vesikari Scale score (MVS) was used to evaluate the disease severity. The objectives are to identify the Genogroup and Genotype distribution and to compare illness severity between the GI and GII genogroups and to complete further analyses comparing the GII genotypes identified. GII.4 was the genotype most commonly identified. Children with GII.4 had higher MVS scores (12.0 (10.0, 14.0; p = 0.002)) and more prolonged diarrheal (5 days (3.0, 7.8)) and vomiting (3.2 days (1.7, 5.3; p < 0.001)) durations compared to other non GII.4 strains. The predominant strain varied by year with GII.4 Sydney[P31] predominant in 2014/15, GII.4 Sydney[P16] in 2015/16 and 2017/18, and GII.3[P12] in 2016/17. Genogroup II norovirus strains predominated in children with AGE with variance between years; clinical severity associated with different strains varied with episodes being most severe among GII.4 infected children.
Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine
Authors: Zhu Fang-Chao , Lian Chun-Ang , He Li-Sheng
Published: November 2020

Intestinal bacterial communities are highly relevant to the digestion, nutrition, growth, reproduction, and immunity of animals, but little is known about the composition and function of intestinal microbiota in deep-sea invertebrates. In this study, the intestinal microbiota of six holothurian Molpadia musculus were investigated, showing that their midguts were predominantly occupied by Izemoplasmatales bacteria. Using metagenomic sequencing, a draft genome of 1,822,181 bp was successfully recovered. After comparison with phylogenetically related bacteria, genes involved in saccharide usage and de novo nucleotide biosynthesis were reduced. However, a set of genes responsible for extracellular nucleoside utilization and 14 of 20 amino acid synthesis pathways were completely retained. Under oligotrophic condition, the gut-associated bacterium may make use of extracellular DNA for carbon and energy supplement, and may provide essential amino acids to the host. The clustered regularly interspaced short palindromic repeat (CRISPR) and restriction–modification (RM) systems presented in the genome may provide protection against invading viruses. A linear azol(in)e-containing peptide gene cluster for bacteriocin synthesize was also identified, which may inhibit the colonization and growth of harmful bacteria. Known virulence factors were not found by database searching. On the basis of its phylogenetic position and metabolic characteristics, we proposed that the bacterium represented a novel genus and a novel family within the Izemoplasmatales order and suggested it be named “Candidatus Bathyoplasma sp. NZ”. This was the first time describing host-associated Izemoplasmatales.
Dichloromethane Degradation Pathway from Unsequenced Hyphomicrobium sp. MC8b Rapidly Explored by Pan-Proteomics
Authors: Hayoun Karim , Geersens Emilie , Laczny Cédric C. , Halder Rashi , Lázaro Sánchez Carmen , Manna Abhijit , Bringel Françoise , Ryckelynck Michaël , Wilmes Paul , Muller Emilie E. L. , Alpha-Bazin Béatrice , Armengaud Jean , Vuilleumier Stéphane
Published: November 2020

Several bacteria are able to degrade the major industrial solvent dichloromethane (DCM) by using the conserved dehalogenase DcmA, the only system for DCM degradation characterised at the sequence level so far. Using differential proteomics, we rapidly identified key determinants of DCM degradation for Hyphomicrobium sp. MC8b, an unsequenced facultative methylotrophic DCM-degrading strain. For this, we designed a pan-proteomics database comprising the annotated genome sequences of 13 distinct Hyphomicrobium strains. Compared to growth with methanol, growth with DCM induces drastic changes in the proteome of strain MC8b. Dichloromethane dehalogenase DcmA was detected by differential pan-proteomics, but only with poor sequence coverage, suggesting atypical characteristics of the DCM dehalogenation system in this strain. More peptides were assigned to DcmA by error-tolerant search, warranting subsequent sequencing of the genome of strain MC8b, which revealed a highly divergent set of dcm genes in this strain. This suggests that the dcm enzymatic system is less strongly conserved than previously believed, and that substantial molecular evolution of dcm genes has occurred beyond their horizontal transfer in the bacterial domain. Our study showed the power of pan-proteomics for quick characterization of new strains belonging to branches of the Tree of Life that are densely genome-sequenced.
Importance of the Farm Environment and Wildlife for Transmission of Campylobacter jejuni in A Pasture-Based Dairy Herd
Authors: Rapp Delphine , Ross Colleen , Hea Shen-Yan , Brightwell Gale
Published: November 2020

Cattle are an established reservoir of the foodborne bacterial pathogen Campylobacter jejuni. Our six-month study aimed to evaluate sources and pathways governing long-term presence of C. jejuni in a pasture-based dairy herd. C. jejuni was detected in all sample types (soil, pasture, stock drinking water, bird, rodents and cow faeces). It was persistently detected from cow (54%; 49/90 samples) and bird (36%; 77/211) faeces. Genetic comparison of 252 C. jejuni isolates identified 30 Multi-Locus Sequence Types (ST). ST-61 and ST-42 were persistent in the herd and accounted for 43% of the cow isolates. They were also detected on pasture collected from fields both recently and not recently grazed, indicating that grazed pasture is an important pathway and reservoir for horizontal transmission among cows. ST-61 accounted for 9% of the bird isolates and was detected at four of the six sampling events, suggesting that bird populations might contribute to the cycling of ruminant-adapted genotypes on-farm. Overall, the results indicated that management of grazed pasture and supplementary feed contaminated by bird droppings could be targeted to effectively reduce transmission of C. jejuni to dairy herds, the farm environment and ultimately to humans.
Resolved Influenza A Virus Infection Has Extended Effects on Lung Homeostasis and Attenuates Allergic Airway Inflammation in a Mouse Model
Authors: Wu Qingyu , Jorde Ilka , Kershaw Olivia , Jeron Andreas , Bruder Dunja , Schreiber Jens , Stegemann-Koniszewski Sabine
Published: November 2020

Allergic airway inflammation (AAI) involves T helper cell type 2 (Th2) and pro-inflammatory responses to aeroallergens and many predisposing factors remain elusive. Influenza A virus (IAV) is a major human pathogen that causes acute respiratory infections and induces specific immune responses essential for viral clearance and resolution of the infection. Beyond acute infection, IAV has been shown to persistently affect lung homeostasis and respiratory immunity. Here we asked how resolved IAV infection affects subsequently induced AAI. Mice infected with a sublethal dose of IAV were sensitized and challenged in an ovalbumin mediated mouse model for AAI after resolution of the acute viral infection. Histological changes, respiratory leukocytes, cytokines and airway hyperreactivity were analyzed in resolved IAV infection alone and in AAI with and without previous IAV infection. More than five weeks after infection, we detected persistent pneumonia with increased activated CD4+ and CD8+ lymphocytes as well as dendritic cells and MHCII expressing macrophages in the lung. Resolved IAV infection significantly affected subsequently induced AAI on different levels including morphological changes, respiratory leukocytes and lymphocytes as well as the pro-inflammatory cytokine responses, which was clearly diminished. We conclude that IAV has exceptional persisting effects on respiratory immunity with substantial consequences for subsequently induced AAI.
Petrogenesis of the Microgranular Enclaves and Their Host Granites from the Xitian Intrusion in South China: Implications for Geodynamic Setting and Mineralization
Authors: He Miao , Liu Qing , Hou Quanlin , Sun Jinfeng , Yan Quanren
Published: November 2020

The South China Block had experienced a significant tectonic transition during the Mesozoic in response to the subduction of the Paleo- and the Pacific Ocean. Large-scale granitic intrusions with massive mineralization are widespread in South China, and their tectonic settings are not defined. The Xitian intrusion is ideal for probing the geodynamic setting and mineralization in South China because they comprise an abundance of microgranular enclaves (MEs) and diverse types of granite associated with mineralization. Age determined by zircon U-Pb dating suggests that the MEs and their host granites are coeval within error, of ca. 152 Ma. The MEs have a similar initial Hf-O isotopic composition as host granites, and the rapid cooling mineral textures indicate that they are autoliths. Geochemical data show that the host granites are high-K, calc-alkaline, and transitional from metaluminous to peraluminous, slightly enriched in light rare earth elements (LREEs) relative to heavy rare earth elements (HREEs), with obvious negative Eu anomalies, belonging to I-type. The Nb/Ta and Zr/Hf ratios indicate the volatile penetrates the magmatic-forming process, and the fluid with abundant volatile could extract metal element effectively from the mantle.
Odikhinchaite, Na9Sr3[(H2O)2Na]Ca6Mn3Zr3NbSi (Si24O72)O(OH)3(CO3)·H2O, a New Eudialyte-Group Mineral from the Odikhincha Intrusion, Taimyr Peninsula, Russia
Authors: Gritsenko Yuliya D. , Chukanov Nikita V. , Aksenov Sergey M. , Pekov Igor V. , Varlamov Dmitry A. , Pautov Leonid A. , Vozchikova Svetlana A. , Ksenofontov Dmitry A. , Britvin Sergey N.
Published: November 2020

The new eudialyte-group mineral, odikhinchaite, was discovered in a peralkaline pegmatite vein hosted by melteigite at the Odikhincha ultrabasic alkaline–carbonatite intrusion, Taimyr Peninsula, Krasnoyarsk Krai, Russia. Associated minerals are orthoclase, albite, aegirine, cancrinite, ancylite-(Ce), catapleiite, and wadeite. Odikhinchaite occurs as dense rosette-like aggregates up to 11 mm across, consisting of split lamellar individuals. The mineral is translucent to transparent, deep purple, with vitreous luster. Odikhinchaite is brittle, with uneven fracture; distinct cleavage on (001) is observed. Hardness determined by the micro-indentation method is equal to 430 kgf/mm2; the Mohs hardness is 5. D(meas.) is 2.97(1) g·cm−3, D(calc.) is 3.04 g·cm–3. Odikhinchaite is optically uniaxial (–), ω = 1.638(2), ε = 1.630(2). The IR spectrum shows the presence of the IVMn2+O4 polyhedra, H2O molecules and CO32– anions. The chemical composition is (electron microprobe, H2O determined by the modified Penfield method, CO2 determined by selection sorption of gaseous annealing products; wt%): Na2O 9.25, K2O 0.59, CaO 12.77, MnO 5.49, FeO 0.75, MgO 0.24, La2O3 0.38, Ce2O3 0.39, Nd2O3 0.15, Al2O3 0.07, SiO2 44.80, ZrO2 11.13, TiO2 0.07, Nb2O5 4.17, Cl 0.69, CO2 0.90, H2O 2.22, –O = Cl –0.16, total 99.72. The crystal structure was solved using single-crystal X-ray diffraction data. Odikhinchaite is trigonal, space group R3m; the unit-cell parameters are: a = 14.2837(2) Å, c = 30.0697(3) Å, V = 5313.04(12) Å3. The new mineral is isostructural with other 12-layered members of the eudialyte group with the space group R3m. Its crystal chemical formula is (Z = 3): {N1(Na2.58Ca0.42)N2[Na2.37Ca0.51(H2O)0.12]N3(Sr2.00K0.45Na0.35REE0.20)N4Na3N5[(H2O)1.8Na1.2]}{ZZr3M1Ca6M2(Mn2.49Fe2+0.51)[M3Nb(OH)1.82O1.18](M4SiOH)[Si3O9]2[Si9O27]2X1[(CO3)0.53Cl0.47]X2[(H2O)0.6(O,F)0.4]XM4(CO3)0.15. The strongest lines of the powder X-ray diffraction pattern [d, Å (I, %) (hkl)] are: 11.42 (64) (101), 4.309 (41) (205), 3.405 (53) (131), 3.208 (45) (208, 036), 3.167 (44) (217), 2.978 (100) (315), 2.858 (86) (404).
Egyptian Blue Pellets from the First Century BCE Workshop of Kos (Greece): Microanalytical Investigation by Optical Microscopy, Scanning Electron Microscopy-X-ray Energy Dispersive Spectroscopy and Micro-Raman Spectroscopy
Authors: Kostomitsopoulou Marketou Ariadne , Andriulo Fabrizio , Steindal Calin , Handberg Søren
Published: November 2020

This paper aims to expand our understanding of the processes involved in the production of the artificial pigment Egyptian blue through the scientific examination of pigments found in the first century BCE workshop of the Greek island of Kos. There, 136 Egyptian blue pellets were brought to light, including successfully produced pellets, as well as partially successful and unsuccessful products. This study is based on the examination of eighteen samples obtained from pellets of various textures and tones of blue, including light and dark blue pigments, coarse and fine-grained materials, and one unsuccessful pellet of dark green/grey colour. The samples were examined by optical microscopy, scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDS), and micro-Raman spectroscopy. These complementary microanalytical techniques provide localised information about the chemical and mineralogical composition of this multicomponent material, at a single-grain level. The results shed light on the firing procedure and indicate possible sources for raw materials (beach sand, copper alloys), as well as demonstrating the use of a low-alkali starting mixture. Moreover, two different process for the production of light blue pigments were identified: (a) decreased firing time and (b) grinding of the initially produced pellet and mixing with cobalt-containing material.
Prebiotic Potential of Oligosaccharides Obtained by Acid Hydrolysis of α-(1→3)-Glucan from Laetiporus sulphureus: A Pilot Study
Authors: Wiater Adrian , Waśko Adam , Adamczyk Paulina , Gustaw Klaudia , Pleszczyńska Małgorzata , Wlizło Kamila , Skowronek Marcin , Tomczyk Michał , Szczodrak Janusz
Published: November 2020

Increasing knowledge of the role of the intestinal microbiome in human health and well-being has resulted in increased interest in prebiotics, mainly oligosaccharides of various origins. To date, there are no reports in the literature on the prebiotic properties of oligosaccharides produced by the hydrolysis of pure fungal α-(1→3)-glucan. The aim of this study was to prepare α-(1→3)-glucooligosaccharides (α-(1→3)-GOS) and to perform initial evaluation of their prebiotic potential. The oligosaccharides were obtained by acid hydrolysis of α-(1→3)-glucan isolated from the fruiting bodies of Laetiporus sulphureus and then, characterized by HPLC. Fermentation of α-(1→3)-GOS and reference prebiotics was compared in in vitro pure cultures of Lactobacillus, Bifidobacterium, and enteric bacterial strains. A mixture of α-(1→3)-GOS, notably with a degree of polymerization of 2 to 9, was obtained. The hydrolysate was utilized for growth by most of the Lactobacillus strains tested and showed a strong bifidogenic effect, but did not promote the growth of Escherichia coli and Enterococcus faecalis. α-(1→3)-GOS proved to be effective in the selective stimulation of beneficial bacteria and can be further tested to determine their prebiotic functionality.
Deciphering the Role of Filamin B Calponin-Homology Domain in Causing the Larsen Syndrome, Boomerang Dysplasia, and Atelosteogenesis Type I Spectrum Disorders via a Computational Approach
Authors: S. Udhaya Kumar , Sankar Srivarshini , Younes Salma , D. Thirumal Kumar , Ahmad Muneera Naseer , Okashah Sarah Samer , Kamaraj Balu , Al-Subaie Abeer Mohammed , C. George Priya Doss , Zayed Hatem
Published: November 2020

Filamins (FLN) are a family of actin-binding proteins involved in regulating the cytoskeleton and signaling phenomenon by developing a network with F-actin and FLN-binding partners. The FLN family comprises three conserved isoforms in mammals: FLNA, FLNB, and FLNC. FLNB is a multidomain monomer protein with domains containing an actin-binding N-terminal domain (ABD 1–242), encompassing two calponin-homology domains (assigned CH1 and CH2). Primary variants in FLNB mostly occur in the domain (CH2) and surrounding the hinge-1 region. The four autosomal dominant disorders that are associated with FLNB variants are Larsen syndrome, atelosteogenesis type I (AOI), atelosteogenesis type III (AOIII), and boomerang dysplasia (BD). Despite the intense clustering of FLNB variants contributing to the LS-AO-BD disorders, the genotype-phenotype correlation is still enigmatic. In silico prediction tools and molecular dynamics simulation (MDS) approaches have offered the potential for variant classification and pathogenicity predictions. We retrieved 285 FLNB missense variants from the UniProt, ClinVar, and HGMD databases in the current study. Of these, five and 39 variants were located in the CH1 and CH2 domains, respectively. These variants were subjected to various pathogenicity and stability prediction tools, evolutionary and conservation analyses, and biophysical and physicochemical properties analyses. Molecular dynamics simulation (MDS) was performed on the three candidate variants in the CH2 domain (W148R, F161C, and L171R) that were predicted to be the most pathogenic. The MDS analysis results showed that these three variants are highly compact compared to the native protein, suggesting that they could affect the protein on the structural and functional levels. The computational approach demonstrates the differences between the FLNB mutants and the wild type in a structural and functional context. Our findings expand our knowledge on the genotype-phenotype correlation in FLNB-related LS-AO-BD disorders on the molecular level, which may pave the way for optimizing drug therapy by integrating precision medicine.
Formulation of Tioconazole and Melaleuca alternifolia Essential Oil Pickering Emulsions for Onychomycosis Topical Treatment
Authors: Vörös-Horváth Barbara , Das Sourav , Salem Ala’ , Nagy Sándor , Böszörményi Andrea , Kőszegi Tamás , Pál Szilárd , Széchenyi Aleksandar
Published: November 2020

Onychomycosis is a disease that affects many adults, whose treatment includes both oral and topical therapies with low cure rates. The topical therapy is less effective but causes fewer side effects. This is why the development of an effective, easy to apply formulation for topical treatment is of high importance. We have used a nanotechnological approach to formulate Pickering emulsions (PEs) with well-defined properties to achieve site-specific delivery for antifungal drug combination of tioconazole and Melaleuca alternifolia essential oil. Silica nanoparticles with tailored size and partially hydrophobic surface have been synthesized and used for the stabilization of PEs. In vitro diffusion studies have been performed to evaluate the drug delivery properties of PEs. Ethanolic solution (ES) and conventional emulsions (CE) have been used as reference drug formulations. The examination of the antifungal effect of PEs has been performed on Candida albicans and Trichophyton rubrum as main pathogens. In vitro microbiological experimental results suggest that PEs are better candidates for onychomycosis topical treatment than CE or ES of the examined drugs. The used drugs have shown a significant synergistic effect, and the combination with an effective drug delivery system can result in a promising drug form for the topical treatment of onychomycosis.
Effects of Lipid-Based Encapsulation on the Bioaccessibility and Bioavailability of Phenolic Compounds
Authors: Ozkan Gulay , Kostka Tina , Esatbeyoglu Tuba , Capanoglu Esra
Published: November 2020

Phenolic compounds (quercetin, rutin, cyanidin, tangeretin, hesperetin, curcumin, resveratrol, etc.) are known to have health-promoting effects and they are accepted as one of the main proposed nutraceutical group. However, their application is limited owing to the problems related with their stability and water solubility as well as their low bioaccessibility and bioavailability. These limitations can be overcome by encapsulating phenolic compounds by physical, physicochemical and chemical encapsulation techniques. This review focuses on the effects of encapsulation, especially lipid-based techniques (emulsion/nanoemulsion, solid lipid nanoparticles, liposomes/nanoliposomes, etc.), on the digestibility characteristics of phenolic compounds in terms of bioaccessibility and bioavailability.