bluuebunny/crossref_metadata_2025 huggingface dataset:
Similar but Not Identical Resuscitation Trajectories of the Soil Microbial Community Based on Either DNA or RNA after Flooding
Authors: Zhu Yizu , Li Yaying , Zheng Ningguo , Chapman Stephen James , Yao Huaiying
Published: April 2020

Both drought and flooding are unfavorable for soil microorganisms, but nevertheless, are highly relevant to the extreme weather events that have been predicted to increase in the future. The switch of soil water status from drought to flooding can happen rapidly and microbial activity might be either stimulated or further inhibited, but we have insufficient understanding of the underlying microbial processes. Here, we tracked the changes in soil bacterial and fungal abundance and their community structures, assaying the total (DNA-based) and potentially active (RNA-based) communities in response to abrupt flooding of dry soil. Also, rates of soil respiration and enzyme activity were measured after flooding. Results showed that the bacterial community was found to be more responsive than the fungal community to flooding. The bacterial community responses were clearly classified into three distinct patterns in which the intermediate pattern displayed highly phylogenetic clustering. A transient flourish of Bacilli which belongs to Firmicutes was detected at 8–48 h of flooding, suggesting its potential importance in the microbial assemblage and subsequent ecosystem functioning. Finally, the accumulative amount of CO2 released was more closely related than enzyme activity to the change in structure of the bacterial community after flooding. In conclusion, these findings extended our understanding of the underlying soil microbial processes following abrupt water condition changes.
The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination
Authors: Martínez-Ballesta María del Carmen , Egea-Gilabert Catalina , Conesa Encarnación , Ochoa Jesús , Vicente María José , Franco Jose A. , Bañon Sebastián , Martínez Juan J. , Fernández Juan A.
Published: April 2020

Seed is the dissemination unit of plants initiating an important stage in the life cycle of plants. Seed development, comprising two phases: embryogenesis and seed maturation, may define the quality of sown seed, especially under abiotic stress. In this review we have focused on the recent advances in the molecular mechanisms underlying these complex processes and how they are controlled by distinct environmental factors regulating ion homeostasis into the seed tissues. The role of transporters affecting seed embryogenesis and first stages of germination as imbibition and subsequent radicle protrusion and extension were revised from a molecular point of view. Seed formation depends on the loading of nutrients from the maternal seed coat to the filial endosperm, a process of which the efflux is not clear and where different ions and transporters are involved. The clear interrelation between soil nutrients, presence of heavy metals and the ion capacity of penetration through the seed are discussed in terms of ion effect during different germination stages. Results concerning seed priming techniques used in the improvement of seed vigor and radicle emergence are shown, where the use of nutrients as a novel way of osmopriming to alleviate abiotic stress effects and improve seedlings yield is discussed. Novel approaches to know the re-translocation from source leaves to developing seeds are considered, as an essential mechanism to understand the biofortification process of certain grains in order to cope with nutrient deficiencies, especially in arid and semiarid areas. Finally, the role of new genes involved in hormone-dependent processes, oxidative response and water uptake into the seeds during their development or germination, have been described as plant mechanisms to deal with abiotic stresses.
Fusarium Head Blight, Mycotoxins and Strategies for Their Reduction
Authors: Mielniczuk Elżbieta , Skwaryło-Bednarz Barbara
Published: April 2020

Mycotoxins are secondary metabolites of microscopic fungi, which commonly contaminate cereal grains. Contamination of small-grain cereals and maize with toxic metabolites of fungi, both pathogenic and saprotrophic, is one of the particularly important problems in global agriculture. Fusarium species are among the dangerous cereal pathogens with a high toxicity potential. Secondary metabolites of these fungi, such as deoxynivalenol, zearalenone and fumonisin B1 are among five most important mycotoxins on a European and world scale. The use of various methods to limit the development of Fusarium cereal head diseases and grain contamination with mycotoxins, before and after harvest, is an important element of sustainable agriculture and production of safe food. The applied strategies utilize chemical and non-chemical methods, including agronomic, physical and biological treatments. Biological methods now occupy a special place in plant protection as an element of biocontrol of fungal pathogens by inhibiting their development and reducing mycotoxins in grain. According to the literature, Good Agricultural Practices are the best line of defense for controlling Fusarium toxin contamination of cereal and maize grains. However, fluctuations in weather conditions can significantly reduce the effectiveness of plants protection methods against infection with Fusarium spp. and grain accumulation of mycotoxins.
Genotype-Dependent Differences between Cereals in Response to Manganese Excess in the Environment
Authors: Skórka Magdalena , Sieprawska Apolonia , Bednarska-Kozakiewicz Elżbieta , Gawrońska Katarzyna , Kornaś Andrzej , Telk Anna
Published: April 2020

Industrial and agronomic activities lead to oversupply and accumulation of elements in the environment. Relatively little is known about mechanisms of manganese (Mn) triggered stress. In this study, different cultivars of popular cereals wheat, oat, and barley were investigated for their response to excessive Mn. Manganese ions (MnCl2) at 5 and 10 mM were applied to the grains and then to the media on which the plants grew until they developed their first leaf. It was performed ICP MS aiming to understand the mechanism of manganese stress in susceptible and resistant cultivar. Under Mn-stress a decrease in fresh weight of plants was observed, also differences in water content in first leaves, an increase in superoxide dismutases (SOD) and peroxidases (POX) activity, and a significant rise in catalase (CAT) was only characteristic for barley. Increasing Mn concentration resulted in enhancing of manganese superoxide dismutase (Mn-SOD) and copper, zinc superoxide dismutase (Cu/Zn-SOD) bands intensity. The increase in proline content, depending on a balance between pyrroline-5-carboxylate synthase (P5CS), ornithine-d-aminotransferase (OAT), and proline dehydrogenases (PHD) activities, indicated osmotic disorders in all plants and differentiated the studied cereals. Microscopic observations of changes in the structure of plastids and starch accumulation in Mn presence were particularly visible in sensitive cultivars. The study ranked the tested cereals in terms of their tolerance to Mn from the most tolerant wheat through barley and the least tolerant oats.
Deep Functional Profiling Facilitates the Evaluation of the Antibacterial Potential of the Antibiotic Amicoumacin
Authors: Terekhov Stanislav S. , Nazarov Anton S. , Mokrushina Yuliana A. , Baranova Margarita N. , Potapova Nadezhda A. , Malakhova Maja V. , Ilina Elena N. , Smirnov Ivan V. , Gabibov Alexander G.
Published: April 2020

The global spread of antibiotic resistance is forcing the scientific community to find new molecular strategies to counteract it. Deep functional profiling of microbiomes provides an alternative source for the discovery of novel antibiotic producers and probiotics. Recently, we implemented this ultrahigh-throughput screening approach for the isolation of Bacillus pumilus strains efficiently producing the ribosome-targeting antibiotic amicoumacin A (Ami). Proteomics and metabolomics revealed essential insight into the activation of Ami biosynthesis. Here, we applied omics to boost Ami biosynthesis, providing the optimized cultivation conditions for high-scale production of Ami. Ami displayed a pronounced activity against Lactobacillales and Staphylococcaceae, including methicillin-resistant Staphylococcus aureus (MRSA) strains, which was determined using both classical and massive single-cell microfluidic assays. However, the practical application of Ami is limited by its high cytotoxicity and particularly low stability. The former is associated with its self-lactonization, serving as an improvised intermediate state of Ami hydrolysis. This intramolecular reaction decreases Ami half-life at physiological conditions to less than 2 h, which is unprecedented for a terminal amide. While we speculate that the instability of Ami is essential for Bacillus ecology, we believe that its stable analogs represent attractive lead compounds both for antibiotic discovery and for anticancer drug development.
What Resources Do NHS Commissioning Organisations Use to Support Antimicrobial Stewardship in Primary Care in England?
Authors: Allison Rosalie , Lecky Donna M. , Beech Elizabeth , Ashiru-Oredope Diane , Costelloe Céire , Owens Rebecca , McNulty Cliodna A.M.
Published: April 2020

Professional education and public engagement are fundamental components of any antimicrobial stewardship (AMS) strategy. The National Institute for Health and Care Excellence (NICE), Public Health England (PHE), Health Education England (HEE) and other professional organisations, develop and publish resources to support AMS activity in primary care settings. The aim of this study was to explore the adoption and use of education/training and supporting AMS resources within NHS primary care in England. Questionnaires were sent to the medicines management teams of all 209 Clinical Commissioning Groups (CCGs) in England, in 2017. Primary care practitioners in 168/175 (96%) CCGs received AMS education in the last two years. Respondents in 184/186 (99%) CCGs reported actively promoting the TARGET Toolkit to their primary care practitioners; although 137/176 (78%) did not know what percentage of primary care practitioners used the TARGET toolkit. All respondents were aware of Antibiotic Guardian and 132/167 (79%) reported promoting the campaign. Promotion of AMS resources to general practices is currently excellent, but as evaluation of uptake or effect is poor, this should be encouraged by resource providers and through quality improvement programmes. Trainers should be encouraged to promote and highlight the importance of action planning within their AMS training. AMS resources, such as leaflets and education, should be promoted across the whole health economy, including Out of Hours and care homes. Primary care practitioners should continue to be encouraged to display a signed Antibiotic Guardian poster as well as general AMS posters and videos in practice, as patients find them useful and noticeable.
PCSK9 Regulates Nox2-Mediated Platelet Activation via CD36 Receptor in Patients with Atrial Fibrillation
Authors: Cammisotto Vittoria , Pastori Daniele , Nocella Cristina , Bartimoccia Simona , Castellani Valentina , Marchese Cinzia , Sili Scavalli Antonio , Ettorre Evaristo , Viceconte Nicola , Violi Francesco , Pignatelli Pasquale , Carnevale Roberto
Published: April 2020

Background: High levels of proprotein convertase subtilisin/kexin 9 (PCSK9) is predictive of cardiovascular events (CVEs) in atrial fibrillation (AF). We hypothesized that PCSK9 may directly induce platelet activation (PA). Methods: We measured platelet aggregation, recruitment, Thromboxane B2 (TxB2) formation and soluble P-selectin levels as markers of PA and soluble Nox2-derived peptide (sNox2-dp), H2O2, isoprostanes and oxidized Low-Density-Lipoprotein (oxLDL) to analyze oxidative stress (OS) in 88 patients having PCSK9 values < (n = 44) or > (n = 44) 1.2 ng/mL, balanced for age, sex and cardiovascular risk factors. Furthermore, we investigated if normal (n = 5) platelets incubated with PCSK9 (1.0–2.0 ng/mL) alone or with LDL (50 µg/mL) displayed changes of PA, OS and down-stream signaling. Results: PA and OS markers were significantly higher in patients with PCSK9 levels > 1.2 ng/mL compared to those with values < 1.2 ng/mL (p < 0.001). Levels of PCSK9 significantly correlated with markers of PA and OS. Platelets incubation with PCSK9 increased PA, OS and p38, p47 and Phospholipase A2 (PLA2) phosphorylation. These changes were amplified by adding LDL and blunted by CD36 or Nox2 inhibitors. Co-immunoprecipitation analysis revealed an immune complex of PCSK9 with CD36. Conclusions: We provide the first evidence that PCSK9, at concentration found in the circulation of AF patients, directly interacts with platelets via CD36 receptor and activating Nox2: this effect is amplified in presence of LDL.
Graph Dilated Network with Rejection Mechanism
Authors: Yan Bencheng , Wang Chaokun , Guo Gaoyang
Published: April 2020

Recently, graph neural networks (GNNs) have achieved great success in dealing with graph-based data. The basic idea of GNNs is iteratively aggregating the information from neighbors, which is a special form of Laplacian smoothing. However, most of GNNs fall into the over-smoothing problem, i.e., when the model goes deeper, the learned representations become indistinguishable. This reflects the inability of the current GNNs to explore the global graph structure. In this paper, we propose a novel graph neural network to address this problem. A rejection mechanism is designed to address the over-smoothing problem, and a dilated graph convolution kernel is presented to capture the high-level graph structure. A number of experimental results demonstrate that the proposed model outperforms the state-of-the-art GNNs, and can effectively overcome the over-smoothing problem.
A Hip Active Assisted Exoskeleton That Assists the Semi-Squat Lifting
Authors: Wei Wei , Zha Shijia , Xia Yuxuan , Gu Jihua , Lin Xichuan
Published: April 2020

(1) Background: In the case of quick picking and heavy lifting, the carrying action results in a much more active myoelectric signal in the lower back than in an upright stationary one, and there is a high risk of back muscle injury without proper handling skills and equipment. (2) Methods: To reduce the risk of LBP during manual handing tasks, a hip active exoskeleton is designed to assist human manual lifting. A power control method is introduced into the control loop in the process of assisting human transportation. The power curve imitates the semi-squat movement of the human body as the output power of the hip joint. (3) Results: According to the test, the torque can be output according to the wearer’s movement. During the semi-squat lifting process, the EMG (electromyogram) signal of the vertical spine at L5/S1 was reduced by 30–48% and the metabolic cost of energy was reduced by 18% compared the situation of without EXO. (4) Conclusion: The exoskeleton joint output torque can change in an adaptive manner according to the angular velocity of the wearer’s joint. The exoskeleton can assist the waist muscles and the hip joint in the case of the reciprocating semi-squat lifting movement.
Blind Image Deconvolution Algorithm Based on Sparse Optimization with an Adaptive Blur Kernel Estimation
Authors: Yang Haoyuan , Su Xiuqin , Chen Songmao
Published: April 2020

Image blurs are a major source of degradation in an imaging system. There are various blur types, such as motion blur and defocus blur, which reduce image quality significantly. Therefore, it is essential to develop methods for recovering approximated latent images from blurry ones to increase the performance of the imaging system. In this paper, an image blur removal technique based on sparse optimization is proposed. Most existing methods use different image priors to estimate the blur kernel but are unable to fully exploit local image information. The proposed method adopts an image prior based on nonzero measurement in the image gradient domain and introduces an analytical solution, which converges quickly without additional searching iterations during the optimization. First, a blur kernel is accurately estimated from a single input image with an alternating scheme and a half-quadratic optimization algorithm. Subsequently, the latent sharp image is revealed by a non-blind deconvolution algorithm with the hyper-Laplacian distribution-based priors. Additionally, we analyze and discuss its solutions for different prior parameters. According to the tests we conducted, our method outperforms similar methods and could be suitable for dealing with image blurs in real-life applications.
Tropospheric Dust and Associated Atmospheric Circulations over the Mediterranean Region with Focus on Romania’s Territory
Authors: Țîmpu Simona , Sfîcă Lucian , Dobri Radu-Vlad , Cazacu Marius-Mihai , Nita Andrei-Ion , Birsan Marius-Victor
Published: April 2020

The aim of this study is to assess the distribution of dust over the Mediterranean region, with a special focus on the territory of Romania. Two parameters are analyzed—Dust Load (DL) and Aerosol Optical Depth (AOD), the data is obtained from the dust forecast model BSC-DREAM8b v2.0, for the period between December 2015 and February 2019. The main geographical features of dust occurrence in the Mediterranean region are presented at the monthly and annual scale. The results show that, for Romania, the dust load is high from February to June, when it reaches its annual maximum. The atmospheric circulation inducing intense dust events over Romania have also been assessed using an objective classification method. A key element for the dust transport from the Sahara toward South-Eastern Europe is represented by the development of a deep cyclone South of Italy, following thereafter a North-East path towards the Balkan peninsula. The results at the regional scale are analyzed in connection with the aerosol optical properties at the local scale (e.g., aerosol optical depth at 440 nm, Absorption Ångström Exponent and Scattering Ångström Exponent at 440 nm and 675 nm, respectively) retrieved from the Aerosol Robotic Network (AERONET-NASA) for Romania, using data from ACTRIS-RO monitoring sites from Iași, Cluj–Napoca, and Bucharest. The differences between the forecast model and the observational data are also explored. Our results also show that the contribution of the natural mineral dust to air pollution in Romania is small, representing not more than 10% of all kinds of aerosols detected over the observation points from the ACTRIS-RO network.
Isolation and Identification of Fusarium spp., the Causal Agents of Onion (Allium cepa) Basal Rot in Northeastern Israel
Authors: Kalman Ben , Abraham Dekel , Graph Shaul , Perl-Treves Rafael , Meller Harel Yael , Degani Ofir
Published: April 2020

Over the past decade, there have been accumulating reports from farmers and field extension personnel on the increasing incidence and spread of onion (Allium cepa) bulb basal rot in northern Israel. The disease is caused mainly by Fusarium species. Rotting onion bulbs were sampled from fields in the Golan Heights in northeastern Israel during the summers of 2017 and 2018. Tissue from the sampled onion bulbs was used for the isolation and identification of the infecting fungal species using colony and microscopic morphology characterization. Final confirmation of the pathogens was performed with PCR amplification and sequencing using fungi-specific and Fusarium species-specific primers. Four Fusarium spp. isolates were identified in onion bulbs samples collected from the contaminated field: F. proliferatum, F. oxysporum f. sp. cepae, and two species less familiar as causative agents of this disease, F. acutatum and F. anthophilium. Phylogenetic analysis revealed that these species subdivided into two populations, a northern group isolated from white (Riverside cv.) onion bulbs, and a southern group isolated from red (565/505 cv.) bulbs. Pathogenicity tests conducted with seedlings and bulbs under moist conditions proved that all species could cause the disease symptoms, but with different degrees of virulence. Inoculating seeds with spore suspensions of the four species, in vitro, significantly reduced seedlings’ germination rate, hypocotyl elongation, and fresh biomass. Mature onion bulbs infected with the fungal isolates produced typical rot symptoms 14 days post-inoculation, and the fungus from each infected bulb was re-isolated and identified to satisfy Koch’s postulates. The onion bulb assay also reflected the degree of sensitivity of different onion cultivars to the disease. This work is the first confirmed report of the direct and primary cause of Fusarium onion basal rot disease in northeastern Israel. These findings are a necessary step towards uncovering the mycoflora of the diseased onion plants and developing a preventive program that would reduce the disease damage.
Catalysis for the Production of Sustainable Fuels and Chemicals
Authors: Ng Flora T. T. , Dalai Ajay K.
Published: April 2020

The emission of green-house gases and environmental concerns have led to recent research in the use of renewable feedstocks derived from biomass, waste oils and fats as a source for fuels and chemicals [...]
Dental Caries Preventive Considerations: Awareness of Undergraduate Dental Students
Authors: Nassar Hani M.
Published: April 2020

The aim of this study was to assess awareness and knowledge of undergraduate dental students of common caries-related preventive considerations and to highlight these factors in a concise manner to act as a guide for dental practitioners. A sample of 118 undergraduate students at a local government dental school was included. An interactive survey that contains questions related to common preventive strategies against dental caries was presented to the students. The survey contained 22 questions concerning dietary and therapeutic strategies. Students casted their votes using their mobile cellphones. The correct answer for each question was shown to the students, and further discussion was held. Data was collected, and the statistical analysis was conducted using one-sample z- and chi-squared tests at 0.05 significance level. The students answered the questions related to oral hygiene practices, xylitol, and the common knowledge regarding fluoride. The questions related to the use of chlorhexidine, dietary factors, and fluoride formulations were answered mostly incorrectly. The students seemed to grasp details of important concepts of flossing, brushing, reducing frequency of sugar exposure, and the use of fluoride products. Still, more emphasis should be given to increase students’ awareness of dietary guidelines for caries prevention, since adequate knowledge of these modalities is paramount for graduating dentists.
“Helping Nemo!”—Using Augmented Reality and Alternate Reality Games in the Context of Universal Design for Learning
Authors: Stylianidou Nayia , Sofianidis Angelos , Manoli Elpiniki , Meletiou-Mavrotheris Maria
Published: April 2020

This article reports on the main experiences gained from a teaching intervention which utilised the alternate reality game ‘Helping Nemo’ in an augmented reality environment for formative assessment. The purpose of the study was to explore the ways in which the affordances arising from the combination of alternate reality games and augmented reality, situated in the context of Universal Design for Learning, might facilitate students’ learning amongst the aspects of engagement, participation, and response to students’ variability. The study took place in a public primary school located in a rural area of Cyprus. A second-grade class consisting of 24 students aged 7–8 years old was selected to comprise the sample. A qualitative research approach was adopted. The data collection methods included classroom observations and focus groups with the students. Findings gained from the teaching intervention suggest that the creation of a multimodal environment that draws on the principles of Universal Design for Learning and combines the affordances of alternate reality games and augmented reality for formative assessment contributes towards higher levels of engagement and participation in learning of all students, including bilingual students, students with learning disabilities, and students who are currently disengaged.
A Novel Approach towards the Design and Implementation of Virtual Network Based on Controller in Future IoT Applications
Authors: Mehmood Faisal , Ullah Israr , Ahmad Shabir , Kim Do-Hyeun
Published: April 2020

The Internet of Things refers (IoT) to the billions of physical devices around the globe that are connected to the Internet, collecting and sharing data. The overall Internet of Things market is projected to be worth more than 50.6 billion U.S. dollars in 2020. IoT devices possess low processing capabilities, limited memory, limited storage, and minimal network protocol support. With the help of cloud computing technology, we can overcome the limited resources of IoT devices. A lot of research has been conducted on IoT device virtualization to facilitate remote access and control. The concept of virtualization in IoT is to provide a virtual representation of physical devices in the form of virtual objects. IoT devices are more likely to be accessed and communicate through virtual objects in the near future. In this paper, we present the design and implementation of building a virtual IoT network for a smart home. The virtual network is based on virtual objects and IoT controller. We derived the concept from Software Defined Network (SDN) and separated the control plane and data plane in the virtual IoT network. This enhanced the rapid development of diverse applications on top of the virtualization layer by establishing a dynamic end-to-end connection between IoT devices. This article briefly explains the design and development of the virtual network. Results achieved during experiments and performance analysis show that IoT controller enhances the capabilities of a virtual network by dynamically controlling the traffic congestion, handling mapping requests, and routing mechanisms.
Development of Real-Time Implementation of a Wind Power Generation System with Modular Multilevel Converters for Hardware in the Loop Simulation Using MATLAB/Simulink
Authors: Shin Dong-Cheol , Lee Dong-Myung
Published: April 2020

In this study, we propose a wind power generation system model for operating modular multilevel converter (MMC) in a hardware-in-the-loop simulation (HILS) application. The application of the MMC is a system that connects wind power to a grid through high-voltage direct current (HVDC) in the form of back-to-back connected MMCs, whereas a HILS is a system used to test or develop hardware or a software algorithm with real time. A real-time operation model of the MMC is required to conduct a HILS experiment. Although some studies have introduced the HILS model of MMCs for grid connection using PSCAD/EMTDC, it is difficult to find a study in the literature on the model using Matlab/Simulink, which is widely used for power electronic simulation. Hence, in this paper, we propose a real-time implementation model employing a detailed equivalent model (DEM) using MATLAB/Simulink. The equivalent model of both wind power generation system and MMC are presented in this paper. In addition, we describe how to implement components such as a variable resistor that is not provided in the Simulink’s library. The feasibility of the proposed model is demonstrated with real-time operation of a wind power generation system.
A New Adaptive Mass Estimation Approach of Heavy Truck Based on Engine Torque Local Convex Minimum Characteristic at Low Speeds
Authors: Jung DaeYi , Choi Gyoojae
Published: April 2020

This paper proposes a new mass estimation for a vehicle system, utilizing the characteristics of engine torque local convex minimum, where the mass can be estimated based on the driving forces and the longitudinal accelerations only. Fundamentally, this approach generally requires no other information about an aerodynamic effect, a road grade, or a rolling friction, which is usually demanded by the existing well-known longitudinal dynamics and adaptive filter-based estimation methods. The effectiveness of the proposed approach was evaluated and validated by both TruckSim/Simulink co-simulation and actual field test data. It is found that the proposed estimation technique is more favorable for a situation where the vehicle is exposed to low-speed regions. In addition to this new mass estimation strategy, other new and current existing methods were explored and are reviewed here. Moreover, this study suggested a guideline for a hybrid-type mass estimation strategy to predict a mass by combining a new method with an existing one for every speed.
Study of Xoconostle (Opuntia spp.) Powder as Source of Dietary Fiber and Antioxidants
Authors: Arias-Rico José , Cruz-Cansino Nelly del Socorro , Cámara-Hurtado Montaña , López-Froilán Rebeca , Pérez-Rodríguez María Luisa , Sánchez-Mata María de Cortes , Jaramillo-Morales Osmar Antonio , Barrera-Gálvez Rosario , Ramírez-Moreno Esther
Published: April 2020

The objective of this study is to evaluate the nutritional composition, antioxidant properties, and functional characteristics of two cultivars of xoconostle Opuntia xoconostle F.A.C. Weber in Diguet cv. Cuaresmeño (XC) and Opuntia matudae Scheinvar cv. Rosa (XR). The samples were frozen (−32 °C, 48 h), lyophilized (96 h, −55 ± 1 °C, vacuum of 0.040 Mbar), and homogenized (size particle 500 μm) to get the xoconostle powder. Both cultivars (XC and XR) had a high content of carbohydrates characterized by soluble sugars (9.8 ± 0.7 and 29.9 ± 0.5 g/100 g dm) and dietary fiber (30.8 ± 0.7 and 36.8 ± 0.9 g/100 g dm), as well as lower proportions of organic acids, mainly citric acid (18.8 ± 0.0 and 13.6 ± 0.0 mg/100 g dm). These samples also had a high content of phenolic compounds (1580.3 ± 33.1 and 1068.5 ± 70.8 mg GAE/100 g dm), vitamin C (723.1 ± 16 and 320.2 ± 7.5 mg/100 g dm), and antioxidant activity ABTS·+ and DPPH· (between 1348.1 ± 74.0 and 3318.7 ± 178.8 µmol TE/100 g dm). Since xoconostle samples had a high content of dietary fiber, they were characterized by the capacity of water retention (water holding capacity 6.00 ± 0.1 and 5.5 ± 0.2 g H2O/g dm) and gel formation (swelling 5.2 ± 0.0 and 5.5 ± 0.0 g H2O/g dm), related with the retention of lipids and glucose in the food matrix similar to other foods. XR was characterized by a higher amount of dietary fiber, sugars and organic acids, while XC had higher phenols content and antioxidant properties, with higher values of functional properties. Then, our data suggest that both xoconostle cultivars in powder can be used as a functional ingredient for its fiber content and antioxidant properties, contributing with sensorial aspects as flavor and color. Therefore, these highly valued products can be used in the pharmaceutical and food industries.
Flaxseed Enriched Pasta—Chemical Composition and Cooking Quality
Authors: Zarzycki Piotr , Sykut-Domańska Emilia , Sobota Aldona , Teterycz Dorota , Krawęcka Ada , Blicharz-Kania Agata , Andrejko Dariusz , Zdybel Beata
Published: April 2020

Pasta production is a good opportunity for product innovation in different forms. The aim of this work was to assess the use of flaxseed components for pasta production. We examined the chemical composition and cooking quality, at different contents of flaxseed flour (FF) and flaxseed cake (FC), added for pasta processing. The analysis showed that the addition of flaxseed components to the dough caused a substantial difference in the International Commission on Illumination color model (CIE) parameter, compared to control samples. The samples of pasta with FF and FC were darker, redder, and less yellow than the control. The minimum cooking time for the enriched pasta was longer than that for the control pasta, although cooking losses were lower. The increasing content of flaxseed components did not significantly change the weight and volume increase index. The enrichment of pasta with 23% FF and 17% FC yielded good quality pasta. The results of the chemical composition of the flaxseed-enriched pasta indicate considerably enhanced nutritional quality, particularly the levels of protein, fat, and dietary fiber of the pasta, without affecting its quality. Moreover, flaxseed cake can be an important source of nutritional ingredients for pasta production, although it is a by-product of the oil cold pressing technology.
Optimizing High Pressure Processing Parameters to Produce Milkshakes Using Chokeberry Pomace
Authors: Diez-Sánchez Elena , Martínez Antonio , Rodrigo Dolores , Quiles Amparo , Hernando Isabel
Published: April 2020

High hydrostatic pressure is a non-thermal treatment of great interest because of its importance for producing food with additional or enhanced benefits above their nutritional value. In the present study, the effect of high hydrostatic pressure processing parameters (200–500 MPa; 1–10 min) is investigated through response surface methodology (RSM) to optimize the treatment conditions, maximizing the phenol content and antioxidant capacity while minimizing microbiological survival, in milkshakes prepared with chokeberry pomace (2.5–10%). The measurement of fluorescence intensity of the samples was used as an indicator of total phenolic content and antioxidant capacity. The results showed that the intensity of the treatments had different effects on the milkshakes. The RSM described that the greatest retention of phenolic compounds and antioxidant capacity with minimum microbiological survival were found at 500 MPa for 10 min and 10% (w/v) chokeberry pomace. Therefore, this study offers the opportunity to develop microbiologically safe novel dairy products of high nutritional quality.
Pulsed Electric Field Processing of Red Grapes (cv. Rondinella): Modifications of Phenolic Fraction and Effects on Wine Evolution
Authors: Comuzzo Piergiorgio , Voce Sabrina , Grazioli Cristian , Tubaro Franco , Marconi Marco , Zanella Gianmaria , Querzè Marco
Published: April 2020

Pulsed electric field (PEF) is a non-thermal technology able to promote color and polyphenols extraction from grape skins. Most of the publications about PEF in winemaking report data concerning international varieties, poorly considering minor cultivars and the medium/long-term effects of the treatment on wine composition during storage. PEF was applied at different specific energies (2, 10, and 20 kJ kg−1) on grapes of the low-color red cv. Rondinella, after crushing-destemming. Pressing yield, the evolution of color, and total phenolic index (TPI) were measured during skin maceration. Moreover, the wines were characterized for basic compositional parameters, color, anthocyanin profile, phenolic composition (glories indices), metal content (Fe, Cr, and Ni), and sensory characters, two and twelve months after the processing, in comparison with untreated samples and pectolytic enzymes (PE). PEF did not affect fermentation evolution, nor did it modify wine basic composition or metal content. Treatments at 10 and 20 kJ kg−1 led to higher color and TPI in wines, in comparison to PE, because of increased content of anthocyanins and tannins. The sensory evaluation confirmed these findings. Modifications remained stable in wines after twelve months. Glories indices and vitisin A content highlighted greater potential stability of wine color in PEF-treated wines.
Liquid Chromatography Mass Spectrometry Quantification of α-solanine, α-chaconine, and Solanidine in Potato Protein Isolates
Authors: Nielsen Søren D. , Schmidt Jesper M. , Kristiansen Gitte H. , Dalsgaard Trine K. , Larsen Lotte B.
Published: April 2020

For potato proteins to be used as a food ingredient, the level of natural potato defense substances, the glycoalkaloids (GAs), should be limited. In this work, a method is developed for quantification of the two major potato GAs, α-solanine and α-chaconine, as well as for their aglycon form, solanidine, using liquid chromatography–mass spectrometry single quadrupole in single ion monitoring mode. Standard solutions of GA and a food-grade potato protein powder was used to validate the method. A linear correlation between GA concentration and the ion intensity of >0.995 was obtained for all standard solutions. Recovery of GA in spiked samples was within the range 82%–106%. The method for GA quantification was applied to a variety of potato protein isolates. The results showed that total GA increased during the storage of the potatoes. Washing the potato protein isolates using water at a sufficient level was shown to be able to reduce the amount of GA below the threshold of 150 µg g−1, as needed for human consumption.
Perception of the Attributes of Sherry Wine and Its Consumption in Young People in the South of Spain
Authors: Cruces-Montes Serafín J. , Merchán-Clavellino Ana , Romero-Moreno Antonio , Paramio Alberto
Published: April 2020

The wine with the designation of origin “Jerez-Xerez-Sherry” is one of the most produced in Spain and with a greater volume of exports abroad. This study analyzes the preferences of Sherry Wine and its different varieties, based on gender and knowledge and interest in the world of wine. Similarly, the influence of the attributes of Sherry Wine on its choice and consumption is investigated. For this purpose, 1502 participants (1407 wine consumers) were recruited; among the consumers, 58.5% are women, and 74.3% have little knowledge of wine (Mean age 22.6; SD = 3.07; range 18–30). Data collection was done through an online survey. The results reveal that among the types of wines from Sherry, the Fino and the Manzanilla are the most chosen. The young people who have the highest consumption of wine are those who have the most prior knowledge of wine. Also, these young people attribute their choice of wine to intrinsic factors, and even women and connoisseurs are more important in this type of dimension. But the importance of the attributes differs according to the types of wines (Fino/Cream—flavor, Palo Cortado/Pedro Ximénez—color, Amontillado/Manzanilla—price and Oloroso—prizes). These findings will allow establishing measures for their promotion, as well as for the design and specific sales strategies for each type of wine.
Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation
Authors: Uchida Ryota , Kato Michiko , Hattori Yuka , Kikuchi Hiroko , Watanabe Emi , Kobayashi Katsuumi , Nishida Keigo
Published: April 2020

Jabara (Citrus jabara Hort. ex Y. Tanaka) is a type of citrus fruit known for its beneficial effect against seasonal allergies. Jabara is rich in the antioxidant narirutin whose anti-allergy effect has been demonstrated. One of the disadvantages in consuming Jabara is its bitter flavor. Therefore, we fermented the fruit to reduce the bitterness and make Jabara easy to consume. Here, we examined whether fermentation alters the anti-allergic property of Jabara. Suppression of degranulation and cytokine production was observed in mast cells treated with fermented Jabara and the effect was dependent on the length of fermentation. We also showed that 5-hydroxymethylfurfural (5-HMF) increases as fermentation progresses and was identified as an active component of fermented Jabara, which inhibited mast cell degranulation. Mast cells treated with 5-HMF also exhibited reduced degranulation and cytokine production. In addition, we showed that the expression levels of phospho-PLCγ1 and phospho-ERK1/2 were markedly reduced upon FcεRI stimulation. These results indicate that 5-HMF is one of the active components of fermented Jabara that is involved in the inhibition of mast cell activation.
An Envenoming Syndrome from Massive Vespa Stings Induces Multiple Organ Failure
Authors: Chen Tse-Hao , Liao Wan-Ting , Chen Chien-Sheng , Lin Po-Chen , Wu Meng-Yu
Published: April 2020

Envenoming syndrome is a systemic reaction induced by inoculation of large volumes of Hymenoptera venom. The clinical manifestations range from skin allergic reactions to multiple organ failure. Vespid venom-induced toxic reactions and anaphylaxis are the most common lethal mechanism of death, involving acute respiratory failure, acute liver failure, rhabdomyolysis, acute kidney injury, and severe coagulopathy. Multiple organ failure as a consequence of severe venom toxicity is a rare but dangerous complication in victims. Delay of intervention to correct vespid venom-induced toxic reactions may cause catastrophic complications. Here, we describe a case presenting a rare vespid venom-induced multiple organ failure with systemic coagulopathy after massive Vespa attack.
Low Plasma Sphingomyelin Levels Show a Weak Association with Poor Neurological Outcome in Cardiac Arrest Patients: Results from the Prospective, Observational COMMUNICATE Trial
Authors: Boerlin Aurelio , Luescher Tanja , Becker Christoph , Perrig Sebastian , Thommen Emanuel , Widmer Madlaina , Beck Katharina , Vincent Alessia , Tisljar Kai , Bernasconi Luca , Neyer Peter , Schuetz Philipp , Sutter Raoul , Marsch Stephan , Hunziker Sabina
Published: April 2020

There is interest in novel blood markers to improve risk stratification in patients presenting with cardiac arrest. We assessed associations of different plasma sphingomyelin concentrations and neurological outcome in patients with cardiac arrest. In this prospective observational study, adult patients with cardiac arrest were included upon admission to the intensive care unit (ICU). We studied associations of admission plasma levels of 15 different sphingomyelin species with neurological outcome at hospital discharge (primary endpoint) defined by the modified Rankin Scale by the calculation of univariable and multivariable logistic regression models adjusted for age, gender, and clinical shock markers. We included 290 patients (72% males, median age 65 years) with 162 (56%) having poor neurological outcome at hospital discharge. The three sphingomyelin species SM C24:0, SM(OH) C22:1, and SM(OH) C24:1 were significantly lower in patients with poor neurological outcome compared to patients with favorable outcome with areas under the curve (AUC) of 0.58, 0.59, and 0.59. SM(OH) C24:1 was independently associated with poor neurological outcome in a fully-adjusted regression model (adjusted odds ratio per log-transformed unit increase in SM(OH) C24:1 blood level 0.18, 95% CI 0.04 to 0.87, p = 0.033). Results were similar for 1-year mortality. Low admission sphingomyelin levels showed a weak association with poor neurological outcome in patients after cardiac arrest. If validated in future studies, a better understanding of biological sphingomyelin function during cardiac arrest may help to further advance the therapeutic approach and risk stratification in this vulnerable patient group.
Sleep Disordered Breathing in Hypertrophic Cardiomyopathy—Current State and Future Directions
Authors: Venkataraman Shreyas , Karim Shahid , Rajendran Aiswarya , Chahal C. Anwar A. , Somers Virend K.
Published: April 2020

Hypertrophic cardiomyopathy (HCM) is the most common inherited cardiomyopathy and sleep disordered breathing (SDB) is a treatable risk factor that has been seen to occur concurrently, and is known to propagate mortality and morbidity in a number of cardiovascular disease states including heart failure, and indeed hypertrophic cardiomyopathy. In this review, we summarize past studies that explored the simultaneous occurrence of HCM and SDB, and the pathophysiology of SDB in relation to heart failure, arrhythmias, cardiac ischemia and pulmonary hypertension in HCM. The current therapeutic modalities, with the effect of obstructive sleep apnea (OSA) treatment on HCM, are then discussed along with potential future directions.
Upstroke Time Per Cardiac Cycle as A Novel Parameter for Mortality Prediction in Patients with Acute Myocardial Infarction
Authors: Hsu Po-Chao , Lee Wen-Hsien , Tsai Wei-Chung , Chen Ying-Chih , Chi Nai-Yu , Chang Ching-Tang , Chu Chun-Yuan , Lin Tsung-Hsien , Lee Chee-Siong , Lai Wen-Ter , Sheu Sheng-Hsiung , Su Ho-Ming
Published: April 2020

Background: Acute myocardial infarction (AMI) is one of the leading causes of death in the world. How to simply predict mortality for AMI patients is important because the appropriate treatment should be done for the patients with higher risk. Recently, a novel parameter of upstroke time per cardiac cycle (UTCC) in lower extremities was reported to be a good predictor of peripheral artery disease and mortality in elderly. However, there was no literature discussing the usefulness of UTCC for prediction of cardiovascular (CV) and overall mortality in AMI patients. Methods: 184 AMI patients admitted to the cardiac care unit were enrolled. Ankle-brachial index (ABI) and UTCC were measured by an ABI-form device in the same day of admission. Results: The median follow-up to mortality was 71 months. There were 36 CV and 124 overall mortality. Higher UTCC was associated with increased CV and overall mortality after multivariable analysis (P = 0.033 and P < 0.001, respectively). However, ABI was only associated with CV mortality and overall mortality in the univariable analysis but became insignificant after the multivariable analysis. In addition, after adding UTCC into a basic model including important clinical parameters, left ventricular ejection fraction, Charlson comorbidity index, and ABI, we found the basic model + UTCC had a better predictive value for overall mortality than the basic model itself (P < 0.001). Conclusions: Our study is the first one to evaluate the usefulness of UTCC in AMI patients for prediction of long-term mortality. Our study showed UTCC was an independent predictor of long-term CV and overall mortality and had an additive predictive value for overall mortality beyond conventional parameters. Therefore, screening AMI patients by UTCC might help physicians to identify the high-risk group with increased mortality.
Methotrexate Decreases the Level of PCSK9—A Novel Indicator of the Risk of Proatherogenic Lipid Profile in Psoriasis. The Preliminary Data
Authors: Krahel Julita Anna , Baran Anna , Kamiński Tomasz W. , Maciaszek Magdalena , Flisiak Iwona
Published: April 2020

Background: Proprotein convertase subtilisin/kexin type 9 (PCSK9) exerts an important role in inflammatory processes, lipids homeostasis, and cardiometabolic disorders that are closely associated with psoriasis. The aim of the study was to analyze the clinical and diagnostic value of serum PCSK9 concentrations and their connections with disease severity, inflammation, metabolic syndrome, and impact of systemic therapies in psoriatic patients. The study enrolled thirty-five patients with active plaque-type psoriasis and eighteen healthy volunteers served as controls. Blood samples were obtained before and after 12 weeks of treatment with methotrexate or acitretin. Serum PCSK9 concentrations were measured by the ELISA (Enzyme-Linked Immunosorbent Assay) commercial kits. Morphological and biochemical parameters were assayed using routine laboratory techniques. Psoriatic patients showed significantly elevated levels of PCSK9 compared to controls (p < 0.01), mostly in patients with a mild and moderate course of psoriasis. PCSK9 concentrations correlated positively with BMI and triglyceride levels (p < 0.05). Interestingly, PCSK9 had a strong negative correlation with low-density lipoprotein levels and total cholesterol (p < 0.05). Three months of monotherapy with methotrexate significantly reduced PCSK9 level (p < 0.05), on the contrary, the acitretin group showed a further increase of PCSK9 levels (p < 0.05). PCSK9 seems to be a novel marker of psoriasis and a putative explanation of lipid disturbances, which are common in patients with psoriasis and are vital for the further developing of metabolic syndrome. Methotrexate should be considered as a treatment of choice in patients with an elevated PCSK9 concentration.