bluuebunny/crossref_metadata_2025 huggingface dataset:
Urine metabolomics phenotyping and urinary biomarker exploratory in mild cognitive impairment and Alzheimer’s disease
Authors: Wang Yuye , Sun Yu , Wang Yu , Jia Shuhong , Qiao Yanan , Zhou Zhi , Shao Wen , Zhang Xiangfei , Guo Jing , Song Xincheng , Niu Xiaoqian , Peng Dantao
Published: December 2023

Introduction

Alzheimer’s disease is a prevalent disease with a heavy global burden and is suggested to be a metabolic disease in the brain in recent years. The metabolome is considered to be the most promising phenotype which reflects changes in genetic, transcript, and protein profiles as well as environmental effects. Aiming to obtain a comprehensive understanding and convenient diagnosis of MCI and AD from another perspective, researchers are working on AD metabolomics. Urine is more convenient which could reflect the change of disease at an earlier stage. Thus, we conducted a cross-sectional study to investigate novel diagnostic panels.

Methods

We first enrolled participants from China-Japan Friendship Hospital from April 2022 to November 2022, collected urine samples and conducted an LC–MS/MS analysis. In parallel, clinical data were collected and clinical examinations were performed. After statistical and bioinformatics analyzes, significant risk factors and differential urinary metabolites were determined. We attempt to investigate diagnostic panels based on machine learning including LASSO and SVM.

Results

Fifty-seven AD patients, 43 MCI patients and 62 CN subjects were enrolled. A total of 2,140 metabolites were identified among which 125 significantly differed between the AD and CN groups, including 46 upregulated ones and 79 downregulated ones. In parallel, there were 93 significant differential metabolites between the MCI and CN groups, including 23 upregulated ones and 70 downregulated ones. AD diagnostic panel (30 metabolites+ age + APOE) achieved an AUC of 0.9575 in the test set while MCI diagnostic panel (45 metabolites+ age + APOE) achieved an AUC of 0.7333 in the test set. Atropine, S-Methyl-L-cysteine-S-oxide, D-Mannose 6-phosphate (M6P), Spiculisporic Acid, N-Acetyl-L-methionine, 13,14-dihydro-15-keto-tetranor Prostaglandin D2, Pyridoxal 5’-Phosphate (PLP) and 17(S)-HpDHA were considered valuable for both AD and MCI diagnosis and defined as hub metabolites. Besides, diagnostic metabolites were weakly correlated with cognitive functions.

Discussion

In conclusion, the procedure is convenient, non-invasive, and useful for diagnosis, which could assist physicians in differentiating AD and MCI from CN. Atropine, M6P and PLP were evidence-based hub metabolites in AD.
The origins and dynamic changes of C3- and S100A10-positive reactive astrocytes after spinal cord injury
Authors: Zhao Qing , Ren Yi-long , Zhu Yan-jing , Huang Rui-qi , Zhu Rong-rong , Cheng Li-ming , Xie Ning
Published: December 2023

Accaumulating studies focus on the effects of C3-positive A1-like phenotypes and S100A10-positive A2-like phenotypes of reactive astrocytes on spinal cord injury (SCI), however the origins and dynamic changes of C3- and S100A10-positive reactive astrocytes after SCI remain poorly understood. Through transgenic mice and lineage tracing, we aimed to determine the origins of C3- and S100A10-positive reactive astrocytes. Meanwhile, the distribution and dynamic changes in C3- and S100A10-positive reactive astrocytes were also detected in juvenile and adult SCI mice models and cultured astrocytes. Combing with bulk RNA sequencing (RNA-seq), single-cell RNA sequencing (scRNA-seq) and bioinformatic analysis, we further explored the dynamic transcripts changes of C3- and S100A10-positive reactive astrocytes after SCI. We confirmed that resident astrocytes produced both C3- and S100A10-positive reactive astrocytes, whereas ependymal cells regenerated only S100A10-positive reactive astrocytes in lesion area. Importantly, C3-positive reactive astrocytes were predominantly activated in adult SCI mice, while S100A10-positive reactive astrocytes were hyperactivated in juvenile mice. Furthermore, we observed that C3- and S100A10-positive reactive astrocytes had a dynamic transformation process at different time in vitro and vivo, and a majority of intermediate states of C3- and S100A10-positive reactive astrocytes were found during transformation. RNA-seq and scRNA-seq results further confirmed that the transcripts of C3-positive reactive astrocytes and their lipid toxicity were gradually increased with time and age. In contrast, S100A10-positive reactive astrocytes transcripts increased at early time and then gradually decreased after SCI. Our results provide insight into the origins and dynamic changes of C3- and S100A10-positive reactive astrocytes after SCI, which would be valuable resources to further target C3- and S100A10-positive reactive astrocytes after SCI.
Astrocytes are involved in the formation of corpora amylacea-like structures from neuronal debris in the CA1 region of the rat hippocampus after ischemia
Authors: Riew Tae-Ryong , Hwang Ji-Won , Jin Xuyan , Kim Hong Lim , Jung Sharon Jiyoon , Lee Mun-Yong
Published: December 2023

Recently, we demonstrated that the corpora amylacea (CA), a glycoprotein-rich aggregate frequently found in aged brains, accumulates in the ischemic hippocampus and that osteopontin (OPN) mediates the entire process of CA formation. Therefore, this study aimed to elucidate the mechanisms by which astrocytes and microglia participate in CA formation during the late phase (4–12 weeks) of brain ischemia. Based on various morphological analyses, including immunohistochemistry, in situ hybridization, immunoelectron microscopy, and correlative light and electron microscopy, we propose that astrocytes are the primary cells responsible for CA formation after ischemia. During the subacute phase after ischemia, astrocytes, rather than microglia, express Opn messenger ribonucleic acid and OPN protein, a surrogate marker and key component of CA. Furthermore, the specific localization of OPN in the Golgi complex suggests that it is synthesized and secreted by astrocytes. Astrocytes were in close proximity to type I OPN deposits, which accumulated in the mitochondria of degenerating neurons before fully forming the CA (type III OPN deposits). Throughout CA formation, astrocytes remained closely attached to OPN deposits, with their processes exhibiting well-developed gap junctions. Astrocytic cytoplasmic protein S100β, a calcium-binding protein, was detected within the fully formed CA. Additionally, ultrastructural analysis revealed direct contact between astroglial fibrils and the forming facets of the CA. Overall, we demonstrated that astrocytes play a central role in mediating CA formation from the initial stages of OPN deposit accumulation to the evolution of fully formed CA following transient ischemia in the hippocampus.
Temporal trends of migraine and tension-type headache burden across the BRICS: implications from the Global Burden of Disease study 2019
Authors: Zhang Yuan-jie , Li Xin-yu , Guo Zhi-lin
Published: December 2023

Background

Headache disorders have become a significant global public health issue, with a notably high prevalence observed in developing countries. However, few studies have assessed headache disorders trends in Brazil, Russia, India, China and South Africa (BRICS). This study aimed to assess the prevalence of headache disorders in individuals across the BRICS, spanning the years 1990 to 2019.

Methods

We obtained headache disorders data from the Global Burden of Disease 2019 study (GBD2019). This evaluation examined incidence rates, prevalence, and disability-adjusted life-years (DALYs) for migraine and tension-type headache (TTH) across demographic factors like age, gender, year, and country. Migraine and TTH were diagnosed based on the International Classification of Headache Disorders (ICHD-3) criteria. We used disease codes from the International Classification of Diseases, 10th revision to identify migraine and TTH cases. Statistical analyzes included calculating age-standardized rates and estimated annual percentage changes. Future disease burden was projected using a log-linear age-period-cohort model.

Results

In 2019, India had the highest prevalence of migraine (213890207.93 cases) and TTH (374,453,700 cases). Brazil had the highest migraine age-standardized prevalence rate (18,331 per 100,000) and incidence rate (1,489 per 100,000). For TTH, India had the highest prevalence (26,160 per 100,000) while Russia had the highest incidence (11,512 per 100,000). From 1990 to 2019, China showed the greatest increase in migraine and TTH prevalence. India had the highest migraine (7,687,692) and TTH (741,392) DALYs in 2019.

Conclusion

Migraine and TTH remain highly prevalent in BRICS nations, inflicting considerable disability burden. While India and China face mounting disease prevalence, Brazil contends with high incidence rates. Tailored interventions based on country-specific epidemiological profiles are warranted to mitigate the public health impact.
AngoraPy: A Python toolkit for modeling anthropomorphic goal-driven sensorimotor systems
Authors: Weidler Tonio , Goebel Rainer , Senden Mario
Published: December 2023

Goal-driven deep learning increasingly supplements classical modeling approaches in computational neuroscience. The strength of deep neural networks as models of the brain lies in their ability to autonomously learn the connectivity required to solve complex and ecologically valid tasks, obviating the need for hand-engineered or hypothesis-driven connectivity patterns. Consequently, goal-driven models can generate hypotheses about the neurocomputations underlying cortical processing that are grounded in macro- and mesoscopic anatomical properties of the network's biological counterpart. Whereas, goal-driven modeling is already becoming prevalent in the neuroscience of perception, its application to the sensorimotor domain is currently hampered by the complexity of the methods required to train models comprising the closed sensation-action loop. This paper describes AngoraPy, a Python library that mitigates this obstacle by providing researchers with the tools necessary to train complex recurrent convolutional neural networks that model the human sensorimotor system. To make the technical details of this toolkit more approachable, an illustrative example that trains a recurrent toy model on in-hand object manipulation accompanies the theoretical remarks. An extensive benchmark on various classical, 3D robotic, and anthropomorphic control tasks demonstrates AngoraPy's general applicability to a wide range of tasks. Together with its ability to adaptively handle custom architectures, the flexibility of this toolkit demonstrates its power for goal-driven sensorimotor modeling.
Blood lipids, lipid-regulatory medications, and risk of bladder cancer: a Mendelian randomization study
Authors: Cheng Zhang , Ye Fangdie , Liang Yingchun , Xu Chenyang , Zhang Zheyu , Ou Yuxi , Chen Xinan , Dai Xiyu , Mou Zezhong , Li Weijian , Chen Yiling , Zhou Quan , Zou Lujia , Mao Shanhua , Jiang Haowen
Published: December 2023

Background

The influences of blood lipids and lipid-regulatory medications on the risk of bladder cancer have long been suspected, and previous findings remain controversial. We aimed to assess the causality between blood lipids or lipid-regulatory medications and bladder cancer susceptibility by means of a comprehensive Mendelian Randomization (MR) study.

Methods

Genetic proxies from genome-wide association studies (GWAS) of four blood lipid traits and lipid-lowering variants in genes encoding the targets of lipid-regulatory medications were employed. The largest ever GWAS data of blood lipids and bladder cancer involving up to 440,546 and 205,771 individuals of European ancestry were extracted from UK Biobank and FinnGen Project Round 6, respectively. A two-sample bidirectional MR study was performed using the inverse variance weighted as the main method. The heterogeneity, horizontal pleiotropy, MR Steiger, and leave-one-out analyses were also conducted as sensitivity tests.

Results

There was indicative evidence that genetically predicted low-density lipoprotein cholesterol (LDL-C) affected bladder cancer susceptibility based on 146 single nucleotide polymorphisms (SNPs) with an odds ratio (OR) of 0.776 (95% confidence interval [CI] = 0.625–0.965, p = 0.022). However, this result became non-significant after two SNPs that possibly drove the effect were removed as demonstrated by leave-one-out analysis. The reversed MR analysis suggested that bladder cancer could not affect serum lipid levels. No causal relationship was found between the lipid-lowering effect of lipid-regulatory medications (fibrates, probucol, statins, ezetimibe, proprotein convertase subtilisin/kexin type 9 [PCSK9] inhibitors, and evinacumab) and the risk of bladder cancer. No heterogeneity or pleiotropy was found (all p > 0.05).

Conclusion

This MR study revealed for the first time, using the most recent and comprehensive GWAS data to date, that genetically predicted total cholesterol (TC) and the lipid-lowering effect of lipid-regulatory medications had no causal association with bladder cancer susceptibility. We also verified claims from early studies that low-density lipoprotein cholesterol (HDL-C), LDL-C, and triglyceride (TG) are not related to bladder cancer susceptibility either. The current study indicated that lipid metabolism may not be as important in the tumorigenesis of bladder cancer as previously believed.
Sequential PET/CT and pathological biomarker crosstalk predict response to PD-1 blockers alone or combined with sunitinib in propensity score-matched cohorts of cancer of unknown primary treatment
Authors: Youlong Wang , Huang Qi , Zhong Guangqing , Lv Jun , Guo Qinzhi , Ma Yifei , Wang Xinjia , Zeng Jiling
Published: December 2023

Introduction

The efficacy of immune checkpoint inhibitors (ICIs), including toripalimab and pembrolizumab, has not been confirmed in the treatment of cancer of unknown primary (CUP), which has a very poor prognosis. Combined with anti-angiogenic therapies, ICIs are hypothesized to be effective in prolonging overall survival. The study aims to give evidence on the treatment effects of sunitinib combined with ICIs, find pathological biomarkers associated with changes in volumetric 18F FDG PET/CT parameters, and investigate inner associations among these markers associated with response on PET/CT.

Methods

The study recruited patients receiving combined treatment (ICIs + sunitinib), compared the effects of combined treatment with those of separate treatment and age-matched negative controls, and analyzed propensity score-matched (PSM) pairs. Markers associated with survival were identified, and their inner associations were tested using structural equation modeling.

Results

A total of 292 patients were enrolled in the final analysis, with 53 patients receiving combined treatment. Survival analysis demonstrated significantly prolonged survival in either combined or separate treatment, with the combined arm showing better response when PSM-paired using pre-treatment whole-body PET/CT parameters. The angiogenic markers KDR and VEGF mediate the PD-1 blockade impact on volumetric value changes in positive and negative manners.

Conclusion

The anti-angiogenic agent sunitinib may potentiate PD-1 blockade by diminishing angiogenesis or its downstream effects. The combined separate treatment increased the survival of CUP patients, and the responses could be evaluated using volumetric PET/CT parameters.
Aromatase inhibitors: the journey from the state of the art to clinical open questions
Authors: Generali Daniele , Berardi Rossana , Caruso Michele , Cazzaniga Marina , Garrone Ornella , Minchella Ida , Paris Ida , Pinto Carmine , De Placido Sabino
Published: December 2023

Breast cancer is a major cause of death among females. Great advances have been made in treating this disease, and aromatase inhibitors (AIs) have been recognized as the cornerstone. They are characterized by high efficacy and low toxicity. The authors reviewed the available literature and defined state-of-the-art AI management. This study was designed to assist clinicians in addressing the need to equally weigh patients’ needs and disease control rates in their everyday clinical practice. Today, AIs play a central role in the treatment of hormone receptor-positive breast cancer. In this study, an expert panel reviewed the literature on the use of AIs, discussing the evolution of their use in various aspects of breast cancer, from pre- and postmenopausal early breast cancer to metastatic breast cancer, along with their management regarding efficacy and toxicity. Given the brilliant results that have been achieved in improving survival in everyday clinical practice, clinicians need to address their concerns about therapy duration and the adverse effects they exert on bone health, the cardiovascular system, and metabolism. Currently, in addition to cancer treatment, patient engagement is crucial for improving adherence to therapy and supporting patients’ quality of life, especially in a selected subset of patients, such as those receiving an extended adjuvant or combination with targeted therapies. A description of modern technologies that contribute to this important goal is provided.
Prognostic evaluation of preoperative systemic immune inflammatory index in patients with colorectal cancer
Authors: Zhang Tao , Miao Yong chang
Published: December 2023

Objective

To investigate the impact of preoperative systemic immune inflammatory index (SII) on the clinical prognosis of patients undergoing colorectal cancer (CRC) surgery.

Methods

One hundred and sixty CRC patients who underwent surgical treatment in our gastrointestinal surgery department from January 2019 to May 2023 were collected. ROC curves were applied to determine the sensitivity and specificity of SII, determine the optimal cut-off value into low SII and high SII groups, compare the clinicopathological data of SII patients in the two groups, and analyze the postoperative survival of patients in the two groups using Kaplan-Meier and Log-rank methods. Univariate and multifactor COX proportional risk regression models were used to analyze clinical prognostic factors.

Results

The ROC curve showed that the area under the curve of SII for the evaluation of OS in CRC patients was 0.859, and the best cut-off value was 513.53. There was statistical significance (P < 0.05) in terms of tissue grading and diabetes mellitus in both groups. The Kaplan-Meier survival curves showed that the overall survival rates of the SII<513.53 group and the SII≥513.53 group were 50.88% (29/57) and 32.04% (33/103), and the overall survival rate of the SII<513.53 group was significantly higher than that of the SII≥513.53 group, and the difference was statistically significance (χ2 = 8.375, P=0.004). COX proportional risk regression showed that TNM stage, lymph node metastases, anastomotic fistula and SII were independent risk factors affecting postoperative survival in patients with CRC.

Conclusion

Preoperative SII is an independent prognostic factor for CRC, which is simple, convenient, and non-invasive, and can be used to predict the prognosis of CRC patients.
Enhancing oral squamous cell carcinoma prediction: the prognostic power of the worst pattern of invasion and the limited impact of molecular resection margins
Authors: Hurník Pavel , Režnarová Jana , Chyra Zuzana , Motyka Oldřich , Putnová Barbora Moldovan , Čermáková Zuzana , Blažek Tomáš , Fománek Martin , Gaykalova Daria , Buchtová Marcela , Ševčíková Tereza , Štembírek Jan
Published: December 2023

Objective

Oral squamous cell carcinoma (OSCC) originates from the mucosal lining of the oral cavity. Almost half of newly diagnosed cases are classified as advanced stage IV disease, which makes resection difficult. In this study, we investigated the pathological features and mutation profiles of tumor margins in OSCC.

Methods

We performed hierarchical clustering of principal components to identify distinct patterns of tumor growth and their association with patient prognosis. We also used next-generation sequencing to analyze somatic mutations in tumor and marginal tissue samples.

Results

Our analyses uncovered that the grade of worst pattern of invasion (WPOI) is strongly associated with depth of invasion and patient survival in multivariable analysis. Mutations were primarily detected in the DNA isolated from tumors, but several mutations were also identified in marginal tissue. In total, we uncovered 29 mutated genes, mainly tumor suppressor genes involved in DNA repair including BRCA genes; however none of these mutations significantly correlated with a higher chance of relapse in our medium-size cohort. Some resection margins that appeared histologically normal harbored tumorigenic mutations in TP53 and CDKN2A genes.

Conclusion

Even histologically normal margins may contain molecular alterations that are not detectable by conventional histopathological methods, but NCCN classification system still outperforms other methods in the prediction of the probability of disease relapse.
Mechanism of action and therapeutic targeting of CD30 molecule in lymphomas
Authors: Li Zhaoxia , Guo Wei , Bai Ou
Published: December 2023

At present, the treatment of lymphoma has entered the era of precision medicine, and CD30, as a transmembrane protein, has become an important marker to help the diagnosis and formulation of treatment plans for lymphomas. This protein is widely expressed in various types of lymphomas and can play a role through nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), and other pathways, and ultimately lead to the up-regulation of CD30 expression to give tumor cells a survival advantage. Brentuximab vedotin (BV), as an antibody-drug conjugate (ADC) targeting CD30, is one of the first new drugs to significantly improve survival in patients with CD30+lymphomas. However, the biological function of CD30 has not been fully elucidated. Therefore, this review highlights the CD30-mediated tumor-promoting mechanisms and the molecular factors that regulate CD30 expression. We hope that a better understanding of CD30 biology will provide new insights into clinical treatment and improve the survival and quality of life of lymphoma patients.
The MANGO study: a prospective investigation of oxygen enhanced and blood-oxygen level dependent MRI as imaging biomarkers of hypoxia in glioblastoma
Authors: Brighi Caterina , Waddington David E. J. , Keall Paul J. , Booth Jeremy , O’Brien Kieran , Silvester Shona , Parkinson Jonathon , Mueller Marco , Yim Jackie , Bailey Dale L. , Back Michael , Drummond James
Published: December 2023

Background

Glioblastoma (GBM) is the most aggressive type of brain cancer, with a 5-year survival rate of ~5% and most tumours recurring locally within months of first-line treatment. Hypoxia is associated with worse clinical outcomes in GBM, as it leads to localized resistance to radiotherapy and subsequent tumour recurrence. Current standard of care treatment does not account for tumour hypoxia, due to the challenges of mapping tumour hypoxia in routine clinical practice. In this clinical study, we aim to investigate the role of oxygen enhanced (OE) and blood-oxygen level dependent (BOLD) MRI as non-invasive imaging biomarkers of hypoxia in GBM, and to evaluate their potential role in dose-painting radiotherapy planning and treatment response assessment.

Methods

The primary endpoint is to evaluate the quantitative and spatial correlation between OE and BOLD MRI measurements and [18F]MISO values of uptake in the tumour. The secondary endpoints are to evaluate the repeatability of MRI biomarkers of hypoxia in a test-retest study, to estimate the potential clinical benefits of using MRI biomarkers of hypoxia to guide dose-painting radiotherapy, and to evaluate the ability of MRI biomarkers of hypoxia to assess treatment response. Twenty newly diagnosed GBM patients will be enrolled in this study. Patients will undergo standard of care treatment while receiving additional OE/BOLD MRI and [18F]MISO PET scans at several timepoints during treatment. The ability of OE/BOLD MRI to map hypoxic tumour regions will be evaluated by assessing spatial and quantitative correlations with areas of hypoxic tumour identified via [18F]MISO PET imaging.

Discussion

MANGO (Magnetic resonance imaging of hypoxia for radiation treatment guidance in glioblastoma multiforme) is a diagnostic/prognostic study investigating the role of imaging biomarkers of hypoxia in GBM management. The study will generate a large amount of longitudinal multimodal MRI and PET imaging data that could be used to unveil dynamic changes in tumour physiology that currently limit treatment efficacy, thereby providing a means to develop more effective and personalised treatments.
Application of non-thermal plasma in medicine: a bibliometric and visualization analysis
Authors: Guo Linna , Yan Miao , Gong Hui , Zou Ziang , Henningsen Anders
Published: December 2023

Non-thermal plasma has been widely studied in medicine due to its beneficial effect with low thermal or other damages. Numerous medical research studies have been conducted using non-thermal plasma devices; yet, to date, there is no report summarizing this field as a whole. In this study, we aim to perform a bibliometric analysis to assess the state of research, current research priorities, and emerging trends in non-thermal plasma medicine over the last two decades. Publications related to non-thermal plasma medicine (2002–2022) were searched in Web of Science core collection. Bibliometric analysis and visualization was then performed using R-Bibliometrix and CiteSpace. A total of 725 publications related to non-thermal plasma were identified. The annual number of publications has increased continuously over the past two decades. n the field of non-thermal plasma medicine, Germany, China and the United States dominated. Of all institutions, Leibniz Institute for Plasma Science and Technology published the most papers. The journal with highest citation was JOURNAL OF PHYSICS D-APPLIED PHYSICS. Bekeschus Sander published the highest H-value of articles. “Inactivation”, “Apoptosis”, “Chronic Wound” is the primary focus area of non-thermal plasma medicine, “Differentiation”, “Water”, “Gene Expression” and “Cell Death” were the main keywords of the new research hotspots. In this study, bibliometric methods were used to analyze current research priorities and trends in non-thermal plasma medicine and to identify the countries, institutions, authors, and journals with the greatest influence in the field to enhance collaboration and learning.
Elusive physiological role of prostatic acid phosphatase (PAP): generation of choline for sperm motility via auto-and paracrine cholinergic signaling
Authors: Hanley Peter J.
Published: December 2023

Prostatic acid phosphatase (PAP) exists as two splice variants, secreted PAP and transmembrane PAP, the latter of which is implicated in antinociceptive signaling in dorsal root ganglia. However, PAP is predominantly expressed in the prostate gland and the physiological role of seminal PAP, first identified in 1938, is largely unknown. Here, the author proposes that PAP, following ejaculation, functions to hydrolyze phosphocholine (PC) in seminal fluid and generate choline, which is imported by sperm via a choline transporter and converted to acetylcholine (ACh) by choline acetyltransferase. Auto- and paracrine cholinergic signaling, or choline directly, may subsequently stimulate sperm motility via α7 nicotinic ACh receptors (nAChRs) and contractility of the female reproductive tract through muscarinic ACh receptors (mAChRs). Consistent with a role of PAP in cholinergic signaling, 1) seminal vesicles secrete PC, 2) the prostate gland secretes PAP, 3) PAP specifically catalyzes the hydrolysis of PC into inorganic phosphate and choline, 4) seminal choline levels increase post-ejaculation, 5) pharmacological inhibition of choline acetyltransferase inhibits sperm motility, 6) inhibition or genetic deletion of α7 nAChRs impairs sperm motility, and 7) mAChRs are expressed in the uterus and oviduct (fallopian tube). Notably, PAP does not degrade glycerophosphocholine (GPC), the predominant choline source in the semen of rats and other mammals. Instead, uterine GPC phosphodiesterases may liberate choline from seminal GPC. In summary, the author deduces that PAP in humans, and uterine GPC phosphodiesterases in other mammals, function to generate choline for sperm cholinergic signaling, which promotes sperm motility and possibly contractility of the female reproductive tract.
Effect of grazing and climatic factors on biodiversity-ecosystem functioning relationships in grassland ecosystems - a case study of typical steppe in Inner Mongolia, China
Authors: Zhang Yiran , Wuriliga  , Liu Pengtao , Fan Ruyue , Guo Jing , Liu Li , Ding Yong
Published: December 2023

Biodiversity underpins grassland ecological functions and productive capacities. By studying the mechanisms for the maintenance of species diversity in animal communities, we can provide important theoretical guidance for the optimization of grazing management and biodiversity protection. The typical grassland of Xilingol in Inner Mongolia, China, was used as the experimental area, and a grazing intensity experiment was set up. This consisted of four gradient levels that were grazed by sheep, which were available for continuous monitoring, namely control standard sheep unit·day·hectare-1·year-1 (CK, 0 SSU·d·hm-2y-1), light grazing (LG, 170 SSU·d·hm-2·y-1), moderate grazing (MG, 340 SSU·d·hm-2·y-1), and high grazing (HG, 510 SSU·d·hm-2·y-1). Nine consecutive years of multi-indicator monitoring of vegetation was carried out from 2014–2022, using monitoring data coupled with time series and inter-annual climatic (relative moisture index, RMI) fluctuations. This was done to analyze the impacts of disturbances, such as grazing use and climatic fluctuations, on the diversity of species and above-ground productivity of the community, thereby exploring the relationship between diversity and productivity, and provide possible explanations for the emergence of a range of ecological responses. The statistical analysis methods used were One-way Analysis of Variance (ANOVA), general linear regression and mixed-effects models. The main conclusions of this study are as follows: (1) The grassland in the experimental area under CK had the highest diversity and productivity and the ecosystem was better able to buffer the negative impacts of climatic drought. Furthermore, the effect of climate on productivity and diversity weakened as the intensity of grazing increased. (2) LG to MG had a constant diversity. (3) Grazing utilization changed the relationship between community species diversity and aboveground productivity by releasing spatial community resources, altering the structure of plant communities, weakening competitive exclusion, and strengthening complementary effects. However, under all of the conditions there is a brief stage in the time series when diversity is stimulated to increase, and the higher the grazing intensity, the earlier this occurs.
Plant elicitor peptide induces endocytosis of plasma membrane proteins in Arabidopsis
Authors: Jing Yanping , Zheng Xiaojiang , Sharifi Rouhallah , Chen Jian
Published: December 2023

In plants, the regulation of plasma membrane (PM) dynamics through endocytosis plays a crucial role in responding to external environmental cues and defending against pathogens. The Arabidopsis plant elicitor peptides (Peps), originating from precursor proteins called PROPEPs, have been implicated in various aspects of plant immunity. This study delves into the signaling pathway of Peps, particularly Pep1, and its effect on PM protein internalization. Using PIN2 and BRI1 as PM markers, we demonstrated that Pep1 stimulates the endocytosis of these PM-localized proteins through clathrin-mediated endocytosis (CME). CLC2 and CLC3, two light chains of clathrin, are vital for Pep1-induced PIN2-GFP and BRI1-GFP internalization.The internalized PIN2 and BRI1 are subsequently transported to the vacuole via the trans-Golgi network/early endosome (TGN/EE) and prevacuolar compartment (PVC) pathways. Intriguingly, salicylic acid (SA) negatively regulates the effect of Pep1 on PM endocytosis. This study sheds light on a previously unknown signaling pathway by which danger peptides like Pep1 influence PM dynamics, contributing to a deeper understanding of the function of plant elicitor peptide.
Individual and context-evoked antecedents of exploration-exploitation performance
Authors: Richner Jan , Zagorac-Uremović Zorica , Laureiro-Martínez Daniella
Published: December 2023

A central issue within the Carnegie approach is the exploration-exploitation tension that lies behind organizational adaptation. After decades of research, there is still little understanding of how the combination of individual and context-evoked differences affects exploration-exploitation performance. To address that issue, we build on recent psychological and neuroscientific studies to develop and test an integrative model. The model considers two individual antecedents (personality and cognitive flexibility) and three context-evoked antecedents that take place along different time horizons (recent stress, present emotional states, and present task motivation). We rely on a lab-in-the-field study of 282 leaders within the Swiss Armed Forces—an organization that exhibits the exploration-exploitation tension in an accentuated form. Using structural equation modeling, we conduct a multiple-mediation path analysis aimed at testing complex interactions between multiple variables. Our findings highlight the need to take an integrative approach; cognitive flexibility mediates the positive effect of the personality trait of emotional stability on exploration-exploitation performance, however, both cognitive flexibility and emotional stability play unique, underlying roles in explaining how organizational leaders interpret the context. Emotional stability decreases the negative effect of recent stress on a leader’s cognitive flexibility. Cognitive flexibility, in turn, mediates the effect of the present positive affective signals of task motivation on exploration-exploitation performance. These findings shed new light on our understanding of how adaptive leaders leverage positive and negative context-evoked antecedents that, in turn, affect cognitive flexibility and exploration-exploitation.
A systematic review of cognitive behavioral therapy-based interventions for comorbid chronic pain and clinically relevant psychological distress
Authors: Sanabria-Mazo Juan P. , Colomer-Carbonell Ariadna , Fernández-Vázquez Óscar , Noboa-Rocamora Georgina , Cardona-Ros Gemma , McCracken Lance M. , Montes-Pérez Antonio , Castaño-Asins Juan R. , Edo Sílvia , Borràs Xavier , Sanz Antoni , Feliu-Soler Albert , Luciano Juan V.
Published: December 2023

Objective

Chronic pain frequently co-occurs with clinically relevant psychological distress. A systematic review was conducted to identify the efficacy of cognitive behavioral therapy-based interventions for patients with these comorbid conditions.

Methods

The systematic search was carried out in Medline, PsycINFO, Web of Science, and Scopus up to March 18th, 2023. Four reviewers independently conducted screenings, extraction, and quality assessment.

Results

Twelve randomized controlled trials and one non-randomized controlled trial involving 1,661 participants that examined the efficacy of Cognitive Behavioral Therapy (nine studies), Mindfulness-based Interventions (three studies), Acceptance and Commitment Therapy (one study), and Behavioral Activation Therapy for Depression (one study) were included. Compared to treatment as usual, six out of eight studies of traditional Cognitive Behavioral Therapy reported significant differences in the reduction of depressive symptoms at post-treatment (d from 1.31 to 0.18) and four out of six at follow-up (d from 0.75 to 0.26); similarly, five out of six reported significant differences in the reduction of anxiety symptoms at post-treatment (d from 1.08 to 0.19) and three out of four at follow-up (d from 1.07 to 0.27). Overall, no significant differences between traditional Cognitive Behavioral Therapy and treatment as usual were reported at post-treatment and follow-up in the studies exploring pain intensity and pain catastrophizing.

Conclusion

The available evidence suggests that traditional Cognitive Behavioral Therapy may produce significant benefits for the improvement of depression, anxiety, and quality of life, but not for pain intensity and pain catastrophizing. More evidence is needed to determine the effects of MBI, ACT, and BATD.

Systematic review registration

PROSPERO, CRD42021219921.
Body motion of choral singers
Authors: D'Amario Sara , Ternström Sten , Goebl Werner , Bishop Laura
Published: December 2023

Recent investigations on music performances have shown the relevance of singers' body motion for pedagogical as well as performance purposes. However, little is known about how the perception of voice-matching or task complexity affects choristers' body motion during ensemble singing. This study focussed on the body motion of choral singers who perform in duo along with a pre-recorded tune presented over a loudspeaker. Specifically, we examined the effects of the perception of voice-matching, operationalized in terms of sound spectral envelope, and task complexity on choristers' body motion. Fifteen singers with advanced choral experience first manipulated the spectral components of a pre-recorded short tune composed for the study, by choosing the settings they felt most and least together with. Then, they performed the tune in unison (i.e., singing the same melody simultaneously) and in canon (i.e., singing the same melody but at a temporal delay) with the chosen filter settings. Motion data of the choristers' upper body and audio of the repeated performances were collected and analyzed. Results show that the settings perceived as least together relate to extreme differences between the spectral components of the sound. The singers' wrists and torso motion was more periodic, their upper body posture was more open, and their bodies were more distant from the music stand when singing in unison than in canon. These findings suggest that unison singing promotes an expressive-periodic motion of the upper body.
The effect of animal-assisted intervention on undergraduate students’ perception of momentary stress
Authors: Chute Andrea , Vihos Jill , Johnston Sharon , Buro Karen , Velupillai Nirudika
Published: December 2023

Background

Student mental wellness is a priority in higher education. Animal Assisted Interventions (AAIs’) are gaining momentum in universities across North America (Dell et al., 2015).

Aims

This study explored the relationships between AAIs’, demographic variables, and perceived momentary stress among university students.

Methods

Using a descriptive correlational design, students completed a Perceived Momentary Stress questionnaire that included the Stress Numerical Rating Scale-11 (Stress NRS-11) and the Visual Analog Scale (VAS) to measure perceived stress before and after AAIs’. Data were analyzed using R (4.1.2) (R Core Team, Vienna, Austria) to identify relationships between students’ perceptions of momentary stress, AAIs’ and sociodemographic and demographic variables.

Results

First-year students, female students, and students identifying as sexual minorities were found to benefit the most from AAIs’.

Conclusion

Results from this study reflect relationships between exposure to animal-assisted interventions and student demographic variables.
The nudging effect of AIGC labeling on users’ perceptions of automated news: evidence from EEG
Authors: Liu Yuhan , Wang Shuining , Yu Guoming
Published: December 2023

Introduction

In the context of generative AI intervention in news production, this study primarily focuses on the impact of AI-generated content (AIGC) labeling cues on users’ perceptions of automated news based on nudge theory.

Methods

A 2 (authorship disclosure nudge cues: with vs. without AIGC label) × 2 (automated news type: descriptive vs. evaluative news) within-subject experiment was carried out. Thirty-two participants were recruited to read automated news, evaluate the perceived content trustworthiness, and record with an EEG device.

Results

The results demonstrated that disclosure of AIGC labeling significantly reduced the trustworthiness perception of both fact-based descriptive and opinion-based evaluative news. In EEG, the delta PSD, theta PSD, alpha PSD, and beta PSD with disclosure of AIGC labeling were significantly higher than those without AIGC labeling. Meanwhile, in descriptive news conditions, TAR with AIGC labeling was higher than without AIGC labeling.

Discussion

These results suggested that AIGC labeling significantly improves the degree of attention concentration in reading and deepens the degree of cognitive processing. Users are nudged by AIGC labeling to shift their limited attention and cognitive resources to re-evaluate the information quality to obtain more prudent judgment results. This helps to supplement the theoretical perspective on transparent disclosure nudging in the Internet content governance research field, and it can offer practical guidance to use content labeling to regulate the media industry landscape in the face of AI’s pervasive presence.
Parent–child attachment and mental health in young adolescents: a moderated mediation analysis
Authors: Tan Rong , Yang Yizhi , Huang Tao , Lin Xuanxuan , Gao Hua
Published: December 2023

Introduction

The parent–child attachment has a significant impact on adolescents’ mental health. However, the influence of psychological quality and coping styles on this connection remains unknown. This study examined the relationship between parent–child attachment and adolescent mental health, by exploring the mediating role of psychological quality and the moderating role of coping styles.

Methods

A total of 633 young adolescents participated in this study after signing informed consent. They anonymously completed questionnaires including the Parent and Peer Attachment Scale (Parent Attachment Section), the Coping Styles Inventory for Middle School Students, the Brief Version of the Psychological Quality Inventory for Middle School Students, and the Chinese Middle School Students’Psychological Quality Inventory. After controlling for gender, grade, left-behind category, only-child status, and family structure.

Results

The moderated mediation model yielded the following findings: (a) parent–child attachment significantly and positively predicted adolescents’mental health; (b) psychological quality partially mediated the relationship between parent–child attachment and adolescents’ mental health; (c) the association between psychological quality and mental health was moderated by task-focused coping.

Discussion

This moderation effect was more substantial for students with low task-focused coping behaviors, which aligns with the “exclusionary hypothesis” model. Therefore, our results indicate that parent–child attachment indirectly impacts mental health, influenced by internal and external factors. These findings carry significant implications for safeguarding and promoting adolescents’ mental well-being.
Evaluation of cognitive and psychomotor faculties in relation to mood-related symptoms under the conditions of sleep deprivation
Authors: Sochal Marcin , Ditmer Marta , Białasiewicz Piotr , Turkiewicz Szymon , Karuga Filip Franciszek , Gabryelska Agata
Published: December 2023

Introduction

Deprivation of sleep (DS) has been associated with changes in mood and cognitive function, rapidly but transiently improving the severity of depression symptoms. However, it remains unclear whether there are differences in performance between DS responders and non-responders. The relationship between DS, mood, cognitive, and psychomotor function is also poorly understood.

Methods

Participants (n = 77) underwent a baseline assessment of sleep under the control of polysomnography (PSG). Later they were subjected to DS with actigraphy monitoring. Evaluation of mood as well as completing a battery of tests assessing cognitive functions and eye-hand coordination was conducted four times, pre/post PSG and DS. Participants were further divided into respondents (RE, n = 48) and non-respondents (NR, n = 29) depending on alleviation of depression symptoms severity following DS.

Results

All participants exhibited increased response speed to visual triggers after DS compared to baseline (p = 0.024). Psychomotor vigilance test (PVT) results remained intact in the RE, whereas it was increased in the NR (p = 0.008). Exposure time in the eye-hand coordination test improved in both groups, but total error duration was reduced only in RE individuals (p < 0.001, p = 0.009 for RE and NR, respectively). All subjects were more proficient at trail-making test (p ≤ 0.001 for Part 1 and 2 in all, NR, RE). Stroop test also improved regardless of mood changes after DS (p = 0.007, p = 0.008 for Part 1 and 2, respectively); cognitive interference remained at a similar level within groups (p = 0.059, p = 0.057 for NR and RE, respectively). A positive correlation was observed between the difference in PSG morning/DS morning depression severity and vigilance (R = 0.37, p = 0.001, R = 0.33, p = 0.005, for error duration eye-hand coordination test and PVT total average score, respectively).

Conclusion

RE tend to maintain or improve cognitive function after DS, oppositely to NR. Vigilance in particular might be tightly associated with changes in depression symptoms after DS. Future studies should examine the biological basis behind the response to sleep loss.
What affects the direct economic burden of non-communicable diseases on middle-aged and older adult people in Shaanxi Province?
Authors: Yang Xiaowei , Yan Ju’e , Lai Sha , Shen Chi , Duan Ning
Published: December 2023

Non-communicable diseases (NCDs) are the leading cause of death worldwide. NCDs affect the health status and the quality of life. In addition, continuous NCDs treatment expenses place a heavy economic burden on families and cause huge economic losses to the society. The prevention and treatment of NCDs and reduction of their economic burden are key public health issues. Considering middle-aged and older adult people as the focus, their basic socio-demographic characteristics and health behavior status of this group, and a pooled cross-sections regression model was then used to analyze the main factors affecting the direct economic burden. The results showed that from 2013 to 2018, the prevalence of NCDs among the middle-aged and older adult people in Shaanxi province as well as the direct economic burden of NCDs increased. The effect factors primarily included sex, age, employment status, income level, type of medical insurance, urban or rural residency, level of the health care-providing institutions, visiting times of 2-week, and length of hospital stay. Several measures can be taken to control the onset of NCDs and reduce their direct economic burden.