bluuebunny/crossref_metadata_2025 huggingface dataset:
PSXV-7 The mixture of herbal extracts that change the microbiome of the cattle rumen
Authors: Tuzikov Roman , Nurzhanov Baer , Rysaeff Albert , Duskaev Galimzhan , Rakhmatullin Shamil , Kvan Olga , Shejjda Elena
Published: October 2021

Today, the search for new antibacterial natural agents based on plant raw materials is relevant. These raw materials have promising secondary metabolites that reduce the formation of methane in the ruminant scar. The aim of this study was to evaluate the effect of the following extracts: oak bark+birch leaves (I group) (250 ml/head per day), oak bark + St. John’s wort grass (250 ml / head per day; II group) and birch leaves + St. John’s wort grass (III group; 250 ml/head per day) on the rumen microbiota of large cattle, against the background of control (C). Extracts added to the water of calves with rumen fistula (dairy breed, age 12 months, diet -70 % of hay, 30 % of grain feed, duration is 14 days). The microflora analyzed using high efficiency 16S rRNA (Illumina) gene sequencing. Plant extracts prepared by purification, grinding (1–2 mm) and extraction in a water bath (for 30 min, 70 ° C) and filtration. According to the study results, the inclusion of extracts led to increase of the Bacteroidetes phylum for 1.11–3.69 % in the practical groups in comparison with C. A decrease was obtained for Firmicutes for 2.06–3.55 % (p 0.05) and Proteobacteria. In the I group bacteria of the class Bacteroides increased by 0.9–5.64% and Candidatus Saccharibacteria increased by 0.25% (P = 0.05). In the III group the value increased by 0.64 % (P = 0.05). Compared to C at the level of the family Prevotellaceae dominated in all experimental groups by 1.04–9.11 % (P ≤ 0.05), in group III Bacteroidaceae dominated by 0.27 % and Rikenellaceae by 0.14 %. This contributes to the regulation of opportunistic microorganisms without harmful effects on the rumen microbiota. This research was performed with financial support from the project 0761-2019-0005.
PSIX-19 Effect of substituting groundnut cake with wheat bran mixed with cattle blood meal at ratio 1:1 on performance of broilers
Authors: Aderemi Foluke A
Published: October 2021

This study investigated substituting groundnut cake with wheat bran mixed with cattle blood at ratio1:1 in broilers and it lasted for eight weeks. Seventy-five broiler chicks were allotted randomly to five treatment replicated thrice. Control had 15% GNC, 0% blood meal wheat bran (BMWB) in diets II -V GNC was replaced with BMWB at graded levels of 25,50,75 and 100%. Results revealed that body weight gain and feed intake of diets II and III were similar and (P = 0.05) higher than other diets at both phases. Feed efficiency of diet II was better than others at the starter phase while diet III was better at finisher phase. Haematology and serum chemistry at the finisher phase showed that heamoglobin, white blood cell lymphocyte heterophil and plasma of the birds fed diets II, III, IV and V were (P < 0.05) higher than those on control. The kidney liver and spleen histopathology revealed hepatic and renal degeneration, necrosis and inflammatory responses in diets IV and V. Carcass characteristics showed the wings shanks drumstick leg heart and spleen of birds on BMWB were higher than control. Conclusively it seems BMWB could replace GNC without adverse effect on the broilers at 25% inclusion level
PSX-B-24 Influence of the immunomodulatory drug Azoxivet on the post-vaccination immune response in laying hens
Authors: Sabrekova Valentina , Korenyuga Maxim , Konovalova Elena , Rodionova Natalia
Published: October 2021

Vaccination is a primary way to prevent infectious disease in poultry. The quality of immune response depends on the immune status of the body which, in turn, depends on many endogenous and exogenous factors. This study analyzed the effect of the immunomodulatory drug Azoxivet on the immune response in laying hens after vaccination against Newcastle Disease and Infectious Bursal Disease. There were 4 groups of Loman White cross laying hens (n=20). At age 120 days, all hens habitated in individual cages with an area of 0,15 m2. The conditions of keeping and feeding matched breed requirements. All groups were vaccinated against Newcastle Disease (NDV) (LaSota strain) and Infectious Bursal Disease (IBD) (Winterfield 2512 strain). All groups received Azoxivet (Az) at a dose of 0,3 mg/kg in water three times daily. Blood sera were collected weekly for serological studies using BioChek IBD and NDV test systems. The antibody level of all hens before and after vaccination: 1378 (IBD+Az) vs. 1674,93 (IBD) (P < 0,05), 5194,75 (NDV+Az) vs. 5612,87 (NDV) (P < 0,05). After one week of vaccination: 5931,25 (IBD+Az) vs. 5006,14 (IBD) (P < 0,05), 6207,58 (NDV+Az) vs. 5765,21 (NDV) (P < 0,05). Two weeks: 11086,00 (IBD+Az) vs. 10485,83 (IBD) (P < 0,05), 11255,25 (NDV+Az) vs. 8478,75 (NDV) (P < 0,05). Three weeks: 11478 (IBD+Az) vs. 8286 (IBD) (P < 0,05), 14725 (NDV+Az) vs. 12677 (NDV) (P < 0,05). Four weeks: 12999 (IBD+Az) vs. 1009,67 (IBD) (P < 0,05), 17399 (NDV+Az) vs. 16373,17 (NDV) (P < 0,05). Five weeks: 13601,15 (IBD+Az) vs. 9021,30 (IBD) (P < 0,05), 19671 (NDV+Az) vs. 16309 (NDV) (P < 0,05). Thus, Azaxul had a positive influence on the post-vaccination immune response in laying hens when used after Newcastle Disease and Infectious Bursal Disease vaccines. The results of the study can be used to improve disease prevention in poultry farming.
PSX-B-21 Diagnosis of early mastitis in dairy cows: Somatic cells and bacterial pathogen measuring
Authors: Artemieva Olga , Nikanova Daria A , Kositsin Alexander , Lashneva Irina , Ignatieva Larisa P , Sermyagin Alexander A , Zinovieva Natalia A
Published: October 2021

Mastitis is an inflammatory condition of the breast that can be caused by chemical, physical, traumatic injury or bacteria. In recent years, biomarkers for the mastitis diagnosis have been actively studied. The aim of the study was to assess the relationship between SCC and pathogenic bacteria in milk occurrence. The study carried out by milk samples collected in the experimental herd (Krasnodar region, Russia) from 85 Holsteinized Black-and-White cows. Healthy cows (HC; n = 4), cows at risk for clinical mastitis (RCM, n = 22), subclinical (SCM; n = 25) and clinical mastitis (CM; n = 34) groups were divided by a bacteriological cultivation and SCC level (Fossomatic7 DC). SCC results were logarithmically converted to SCS. Consequent groups were included animals with SCS level: HC (SCS < 4.1), RCM (4.2≤SCS≤5.3), SCM (5.4≤SCS≤6.3), CM (SCS >6.4). HC group of animals showed SCS equal to 3.11±0.28, 4.85±0.44 for RCM, 5.8±0.28 for SCM and 7.43±0.87 for CM. Identification of isolated bacteria species was carried out by conventional biochemical methods using the API20E, APIStaph, API20Strep tests (bioMerieux SA,France). Number of isolated pathogenic bacteria in CM group was 57 strains of which 33.3% were attributed to Enterobacteriaceae, 45.6% to coagulase-negative staphylococci (CONS), 12.3% to S.aureus and 8.8% to Ps.aeruginosa. In SCM group there were assigned 48 strains: 32.5% to Enterobacteriaceae strains, 42.5% to CONS, 20.0% to S.aureus, 5.0% to Ps.aeruginosa. The antibiotic susceptibility was determined according to NCCLS and EUCAST. S.aureus isolates showed the highest sensitivity to erythromycin (4.3% of resistant strains) and the highest resistance to ciprofloxacin (100%), tetracycline (95.0%), rifampicin (88.5%), benzylpenicillin (79.3%), novobiocin (69.2%) and fusidin (65.5%). The primary analysis of SCC and milk microbiological profile can be able to increase the accuracy of mastitis occurrence diagnosing that contributes to taking the right decision for choosing an antibiotic to preserve the cows’ health in Russian cattle population. The study was funded by RSF (project No.21-76-20046)
PSI-4 Animal and agronomic performance while grazing winter stockpiled novel endophyte tall fescue established with three pasture renovation strategies
Authors: Talbott Charlotte L , Harmon Deidre D , Poore Matt H , Franluebbers Alan J , Young Carolyn A , Griffith Emily H
Published: October 2021

Comparing animal and agronomic performance following renovation of toxic-infected tall fescue (TF) to novel endophyte tall fescue (NE) is fundamental to evaluating economic returns and increasing adoption of NE. In 2018, three NE renovation strategies were implemented in a randomized complete block design in Bahama, NC. Strategies included: 1) control (C), 2) renovation to NE after one season of a single specie cover crop (1-SM), 3) renovation to NE after three seasons of a single specie cover crop (3-SM), and 4) renovation to NE after three seasons of a multi-specie cover crop (3-CM). Sixty-four Angus heifers (initial BW 290 kg ± 88) were randomly assigned to treatments and strip-grazed stockpiled fescue for 56-d, two years after NE establishment. Forage yield and nutritive value was measured every 14-d. Data were analyzed using proc GLIMMIX of SAS v9.4. Average daily gain was greatest (P = 0.0024) for cattle grazing 1-SM (0.67 kg/d), and least for 3-CM (0.54 kg/d) and C (0.44 kg/d), which did not differ. 3-SM (0.57 kg/d) did not differ from other treatments. Within the grazing period, forage yield was greatest (P = 0.0001) in 3-CM, 3-SM, and C compared to 1-SM (3053, 2941, 2856 and 2465 kg/ha, respectively). Prior to the initiation of grazing, crude protein concentration was greater (P = 0.0089) for 3-CM (17.5%) compared to C, 1-SM, and 3-SM (15.8%, 15.8% and 15.3%, respectively). Neutral detergent fiber was greater (P = 0.0135) for 3-SM (57%) than C (55.1%) and 3-CM (54.5%), but did not differ from 1-SM (54.6%). Concentration of total digestible nutrients was greater (P = 0.0187) for 3-CM (73.2%) compared to 1-SM (72.1%) and 3-SM (71.9%), but did not differ from C (72.5%). Data suggest animal and agronomic performance for NE was improved compared to TF during winter stockpile grazing in years following renovation.
PSI-15 Embryo viability based upon energy
Authors: Hickerson Sydney L , Wells Cara W , Jesko Noah P , Perkins Thomas L , Killingsworth Russell
Published: October 2021

The objective of this study was to analyze embryo energy release and whether or not it is indicative of survivability upon successful implantation. In both bovine and equine reproduction, fertility is a lowly heritable trait that has an exceedingly large financial impact on the ability to grow genetic lines. In order to increase pregnancy rates, producers must have access to accurate decision-making tools. Nineteen (n = 19) fertilized bovine embryos collected via flushing by licensed veterinarian, Dr. Russell Killingsworth, DVM, in Shamrock, TX, were assessed for energy release using amplification of video with a Nikon Camera and Iolight Microscope. Once embryos were flushed, they were filmed for a thirty-five (35) second observation period. Embryo dimensions were then taken using Image J software. Embryo release of energy was humanly perceptible and measurable by changes in their morphological dimensions. Measurements of significance were taken on the embryo intercellular mass area (ICM), total embryo area, and on the vertical, diagonal, and horizontal axis of the sub-zonal distance, or the distance between the zona pellucida and outer edge of the ICM. All measurements were taken in microns. Once embryos were successfully measured, all were transferred into recipients. Significant findings presented that embryos with a greater ICM, total embryo area, and sub-zonal vertical, diagonal, and horizontal distance established pregnancies more frequently than embryos with less momentous differences in distance (P < 0.05). This research has shown the relationship between embryo energy and pregnancy had a significance of determining pregnancy viability. This has therefore resulted in enhanced prediction of embryo transfer techniques based on embryo quality making pregnancy rates for producers more predictable and consistent.
PSI-11 Effects of a supplemental water source and trace-mineral based electrolyte drinking solution on intake and blood cell count of newly received feedlot calves
Authors: Smithyman Mackenzie M , Gouvêa Vinícius N , Campbell Dayna L , Duff Glenn C , Branine Mark E
Published: October 2021

Oral hydration therapy has been used to improve performance and health of newly received feedlot calves; however, little is known regarding water intake (WI) following arrival at the feedlot. Our objective was to evaluate WI of newly received feedlot calves provided a supplemental water source or a novel nutritional rehydration solution during initial 3 days following arrival. Crossbred heifers (n=180; initial BW = 237 ± 23 kg) were individually weighed after 16 h fasting and sorted into 12 pens (4 pens/treatment). Treatments were: 1) Control (CON): water provided through standard in-pen automatic waterer only (Richie CM480; one waterer/pen); 2) Supplemental water (SUPW): CON + water provided with one additional stock tank/pen; 3) Novel nutritional rehydration solution (NRS): trace-mineral based drinking solution provided with one stock tank/pen as the only water source. Treatments were provided from days 0 to 3 after which supplemental tanks were removed. From days 4 to 14 all heifers had access to the standard in-pen automatic waterer only. The WI was measured daily throughout the trial and BW was recorded at days 0 and 14. Whole blood was collected (5 heifers/pen) on days 0, 3, and 14. Treatments had no effect on DMI or ADG (P ≥ 0.15). SUPW and NRS had greater WI than CON from days 0 to d 3 (P ≤ 0.001), but not from days 4 to 14 nor from days 0 to 14 (P = 0.69). No treatment effect or treatment × day interactions were observed for total red (RBC) or white blood cell counts (WBC; P ≥ 0.19); however, a day effect was present (P < 0.001) and RBC and WBC linearly decreased from day 0 to 14 (P < 0.05). Our preliminary results indicate that providing a supplemental source of water during the initial 3 d after arrival increased total WI and may facilitate rehydration in stressed calves following transit.
PSI-5 Changes in the expression of MyD88, TRAF6, NFKB1, and NFKB1a on digestive tracts of 0 to 42 days-old calves from the tropics of Mexico
Authors: Pérez Mariana Álvarez , Robles-Rodriguez Carolina , Dávalos Laura González , Shimada Armando , Varela Alfredo , Izaguirre Ofelia Mira
Published: October 2021

During the first and second weeks of life, calves are extremely susceptible to enteric diseases, their digestive tract is developing and their thermoregulation is not adequate; these being some factors that can contribute to the lack of their weight gain (Hulbert & Moisá, 2016). In addition, they have a reduced capacity to generate an innate and adaptive immune response, in other words, they are still not immunocompetent (Costa et al., 2017; Hulbert & Moisá, 2016), since they are born with all the essential immune components but many of them are not functional until calves are at least 2 to 4 weeks old (Chase et al., 2008). With the aim to identify the development of the innate immune response through the expression of MyD88, TRAF6, NFKB1, and NFKB1a mRNA, in different ages and regions of the digestive tract, qPCR tests were held. Twelve Zebu crossbred calves of 0, 7, 28, and 42 days of age were sampled (three animals of each age). Tissue samples included: rumen, duodenum, and ileum. Gene expression was determined by quantitative PCR (qPCR). Changes in the expression of TRAF6, NFKB1 and NFKB1a mRNA were different in rumen and ileum (P < 0.05) at day 28 of age. MYD88 mRNA expression was different only in ileum (P < 0.05). MyD88, TRAF6, NFKB1, and NFKB1a mRNA expression of rumen tended to decrease and in the case of ileum to increase with age; however, this was until day 28 and not in the first week of life, as mentioned by some other authors.
PSIII-13 The Effect of the inclusion of liquid brewers yeast during gestation on colostrum quality and passive transfer of immunity in lambs
Authors: Navarrete Julissa , Earing Jennifer E , Holder Mieke , Rickard Justin W , Lugar Drew W W
Published: October 2021

Fourteen Suffolk/Suffolk x Hampshire pregnant ewes were utilized to evaluate the effects of liquid brewers yeast on colostrum quality and the transfer of passive immunity in their offspring. Parainfluenza type 3 (PI3) vaccination was used elicit an immune response. Ewes were vaccinated with PI3 at 33 and 18 d prior to expected lambing day. Ewes were randomly assigned to one of two treatments: late gestation diet (CON) or late gestation diet supplemented with liquid brewers yeast at an average rate of 60 g DM/d (LBY). Ewes were fed treatment diets from 45 d prior to expected lambing to 3 d post lambing. Blood was collected from ewes’ via jugular venipuncture prior to vaccination and within 6 hours postpartum. Blood was collected also from the jugular vein of lambs (n = 22) at 3 d of age. Ewe and lamb blood serum was tested for Bovine Parainfluenza 3 Virus and data on anti-PI3 antibody titers was evaluated. Colostrum was collected within 4 hours postpartum and analyzed for colostral IgG concentrations using a refractometer in % Brix. A PROC GLM model was performed in SAS 9.4, where the model for ewe colostrum quality and lamb anti-PI3 titers analyses included the main effect of treatment diet. All vaccinated ewes were positive for PI3 antibodies (>64 titer) immediately prior to and post lambing. All lambs tested positive for antibody titers for PI3 with no differences between treatment (197.3 for CON and 160.8 for LBY; P = 0.43). The colostral IgG concentrations did not differ between CON (30.4% Brix) and LBY (24.6% Brix) ewes(P = 0.21). These results indicate that, although there were numerical differences, the addition of LBY to the ewes’ diet during late gestation did not significantly improve colostrum quality or passive transfer of immunity in lambs.
PSIII-1 Influence of prenatal or postnatal exposure to gossypol from cottonseed meal on semen quality in commercial boars: a preliminary study on feral hog control
Authors: Benefield Randi F , Mudarra Richard A , Tsai Tsung-Cheng Cheng , Hansen Christopher R , Maxwell Charles V , Rorie Rick W , Littlejohn Brittni P
Published: October 2021

The objective was to examine the influence of prenatal (Experiment 1) or postnatal (Experiment 2) exposure to gossypol from cottonseed meal (CSM) on semen quality in commercial boars. In Experiment 1, pregnant sows (n = 5) were fed a diet containing 0% (n = 1), 0.04% (n = 2), or 0.08% (n = 2) gossypol between d 56 and 86 of gestation. Boars (n = 11) born to sows in each treatment group (0% gossypol n = 3; 0.04% gossypol n = 4; 0.08% gossypol n = 4) were fed a common diet without CSM, and semen was collected at 269±2 d of age using a live sow in estrus. In Experiment 2, boars (n = 21) were fed a diet containing 0%, 0.02%, or 0.04% gossypol between 63±1 and 105±1 d of age (Initial BW: 19.85±0.43 kg). After the treatment period, boars were fed a common diet without CSM, and semen was collected at 238±7 d of age using a breeding dummy. Sperm cell concentration, percentage of motile sperm cells, and percentage of progressively motile sperm cells were analyzed using the MIXED procedure of SAS with treatment as a fixed effect in Experiment 1 and 2 and dam as a random effect in Experiment 1. In Experiment 2, semen was not successfully collected from every boar; therefore, chi-square analysis was used to assess semen collection status between treatment groups using the FREQ procedure of SAS. In Experiment 1, there was no difference in sperm concentration (P = 0.45), percent motility (P = 0.71), or percent progressive motility (P = 0.27) between treatment groups. In Experiment 2, there was no difference in sperm concentration (P = 0.72), percent motility (P = 0.17), or percent progressive motility (P = 0.87) between treatment groups. No difference was observed in boar collection status between treatment groups (P = 0.77). In conclusion, prenatal or postnatal exposure to gossypol from CSM did not influence semen quality in commercial boars.
PSIII-14 The influence of dietary barley supplementation on American Wagyu feedlot performance
Authors: Tangredi Briana V , Thorndyke Meghan P , Guimaraes Octavio , Barrington Rebecca K , Borgerding Shaniece M , Thomas Tyler A , Hallmark Harrison , Yi Loh Huey , Gifford Ryan J , Levenson Jonah R , Engle Terry E
Published: October 2021

Eighty-nine American Wagyu steers were used to evaluate the effects of dietary barley supplementation on feedlot performance. We hypothesized that barley supplementation would increase ADG compared to non-supplemented control animals. The experimental design was a randomized complete block design. Steers were blocked by initial body weight (BW) and randomly assigned within block to one of two treatments. Treatments consisted of 1) Control (no supplemental barley) and 2) Control diet + 0.9 kg∙animal-1∙d-1 of supplemental barley. Steers were housed in feedlot pens (all pens contained n = 4 steers/pen with the exception of one Control pen that contained n=5 steers; 11 replicates/treatment; experimental unit = pen) and fed a traditional American Wagyu finishing diet (DM basis: 68.42% DM, 14.33% CP; TDN: 74.76%, NEg: 1.16 Mcal/kg, 5.25% EE) for 270d. The basal diet consisted of grass hay, corn silage, cracked corn grain, soybean meal, corn distillers grain, white salt, ground limestone, and olive byproduct. Diets were fed once daily in the morning and barely was top dressed to the appropriate pens, immediately after the basal diet was delivered. Steers were individually weighed on d -1 and 0, and approximately every 28 days throughout the 270d experiment. Data were analyzed using a mixed effects model (PROC MIXED, SAS) for a completely randomized block design. Initial pen BW was used as a covariate in the statistical analysis of all performance data and significance was determined at P ≤ 0.05. Initial and final BW, ADG, DMI, and feed:gain were similar across treatments. Therefore, under the conditions of this experiment, these data suggest that barley supplementation for 270d to American Wagyu cattle did not impact overall animal performance.
PSIII-12 The effect of maternal dietary isoflavone supplementation and infection with porcine reproductive and respiratory syndrome virus on the growth performance of offspring
Authors: Chen Xuenan
Published: October 2021

The objective of this experiment was to evaluate the effects of maternal infection with porcine reproductive and respiratory syndrome virus (PRRSV) on piglet prenatal development and pre-weaning growth, and the potential for isoflavone supplementation to alleviate these effects. First parity gilts (n = 24) at gestational day (GD) 65 were allotted to one of three treatments: uninfected and fed a diet devoid of isoflavones (CON), infected with PRRSV and fed the control diet (POS), or infected with PRRSV and fed a diet supplemented with 1,500 ppm soy isoflavones (ISF). Gilts were inoculated intranasally with saline or 2.5×104 TCID50/mL of suspended live PRRSV (NADC20 strain) on GD 70. To determine the effects of PRRSV infection in sows, feed intake (GD 70–114) and rectal temperatures (GD 70–91) were recorded daily, while serum TNF-α concentrations were measured weekly. After farrowing (GD 114 ± 2), two piglets closest to the average litter weight were culled either at birth or weaning (21 d of age) to determine body, muscle, and organ weights of offspring pigs. Hematoxylin and eosin staining was performed on left semitendinosus muscles for determination of muscle cell number and size at each time-point. At birth, whole body, muscle, and organ weights were not different (P > 0.22) between groups except that relative liver weight was increased (P < 0.05) in POS compared with CON piglets. At weaning, ISF reduced (P ≤ 0.05) whole body and muscle weights, but greater (P < 0.05) kidney weight compared with CON, and greater (P < 0.05) relative liver weight compared with CON or POS. However, muscle fiber number and size were not different (P > 0.39) between groups at either time-point. These results suggest maternal PRRSV infection altered offspring organ growth, but dietary ISF did not alleviate the detrimental effects of PRRSV infection in sows or offspring.
PSV-11 The effects of pelleting and extrusion on nutrient composition and protein quality measurements in a variety Canadian pulses
Authors: Cargo-Froom Cara , Shoveller Anna-Kate , Columbus Daniel A , Marinangeli Chris , Kiarie Elijah , Newkirk Rex N , Smilie John , Ai Yongfeng
Published: October 2021

Alternative forms of protein are an important focus in nutrition. This study sought to compare the effects of pelleting and extrusion on nutrient composition and protein quality measurements of Canadian pulses. Pulses used for the study included: 2 pea variety (Amarillo and dunn), lentils, chickpeas, and faba bean. Ingredients were ground through a 10/64” or a 2/64” screen to create a coarse and fine ground product, respectively. Both coarse and fine ground ingredients were pelleted at 60–65, 70–75, and 80–85 C0. Fine ground ingredients were extruded at three different temperatures (110, 130, 150 C0) and two moisture levels (18 and 22%). Samples were collected for all runs at the beginning, middle, and end of each run for both pelleted and extruded samples. Samples were analyzed for proximate analysis, amino acids including lysinoalanine, total and damaged starch, and total dietary fibre (including insoluble and soluble). Data were analyzed using a mixed model via proc glimmix in SAS, where ingredient, process, grind, temperature, and extrusion moisture were treated as fixed effects with different interactions selected based on model investigated. Crude protein content of whole pulses was highest in faba bean and lowest in the Amarillo pea, with faba bean protein content significantly higher than all other pulses, and lentil protein content significantly higher than Amarillo peas (P < 0.05). All pelleting temperatures, nested within grind, significantly increased crude protein content of all pulses compared to whole pulses (P < 0.05). All extrusion moistures significantly increased crude protein content of all pulses compared to whole pulses (P < 0.05) and moisture/temperature interactions were significantly higher for all pulses compared to whole pulses (P < 0.05). Amino acid comparisons produced similar significant results. This suggests that pelleting and extrusion processing can have a positive impact on protein content of pulses and protein quality measurements in pulses.
PSV-15 Using near infrared reflectance spectroscopy to predict lab scoured yield in Rambouillet sheep
Authors: Moody Jordan N , Redden Reid , Pfeiffer Faron A , Pope Ronald , Walker John W
Published: October 2021

Lab scoured yield (LSY) is a major indicator of wool quality. LSY is used for the valuation of wool in commercial settings and can be used by growers as selection criteria for breeding stock. Current laboratory methods for LSY are costly and labor intensive. Evaluation of fleece core samples using Near-Infrared Reflectance Spectroscopy (NIR) may present an efficient, cost-effective alternative to predict LSY. Lamb and yearling fleece core samples from flocks originating from Texas were scanned on a FOSS 6500 spectrometer. Constituent data were obtained from the Bill Sims Wool and Mohair Laboratory using ASTM methodology. LSY ranged from 48–68%. Spectral data were pretreated with a 14 nm moving average and Savitsky-Golay 2nd derivative. Eight outlier spectra were removed. Samples were parsed from the center of the distribution to minimize the Dunn effect creating calibration (n = 108) and test (n = 41) sets. Calibrations were executed using a partial least squares regression on spectra from 1100 to 2492 nm. Test set calibration statistics for LSY were: r2=0.64, RMSE=3.39, and slope=0.91. Independent validation statistics for LSY using spectra for different years were: r2=0.33, RMSE=3.69, and slope=0.29. RMSE for independent validation and lab methods on side samples are similar. Between flock independent validations were less promising. Accuracy of laboratory methods for estimating yield is 2 percentage units. NIRS calibrations can be improved by developing calibration sets with a uniform distribution, which can be difficult within flocks because of the small number of fleeces in the tails of the distribution. These data demonstrate that when calibration and test sets are developed such that test samples are drawn from the calibration population, NIR is a reliable predictor of LSY. However, when test samples are drawn from populations dissimilar to the calibration set, reliability of NIR predictions are greatly reduced.
Mapping Consumers’ Context-Dependent Consumption Preferences: A Multidimensional Unfolding Approach
Authors: Park Junghyun , Kim Minki , Chintagunta Pradeep K
Published: October 2021

How, and to what extent, consumer choices are influenced by the context in which the product is consumed remain important marketing questions. In this article, the authors develop a parsimonious context-dependent multidimensional unfolding model that can accommodate consumers’ context-specific behaviors via ideal points in multiattribute space along with brand locations in that space while accounting for unobserved heterogeneity in consumer behavior. The authors provide an empirical illustration using panel data on beer brand choices in different contexts from U.S. beer consumers. They find more heterogeneity in behavior across social versus nonsocial contexts than across in-home and out-of-home consumption. The authors then show how the model can be used to derive a firm’s optimal direction of brand repositioning given its competitive landscape in the various consumption contexts. Since consumer preferences can be correlated across contexts, they show that a movement toward the ideal point in one context does not necessarily improve the firm’s market competitiveness in other contexts; thereby hurting the brand’s overall performance.
Are Genomic Updates of Well-Studied Species Worth the Investment for Conservation? A Case Study of the Critically Endangered Magdalena River Turtle
Authors: Gallego-García Natalia , Caballero Susana , Shaffer H Bradley
Published: October 2021

As genomic-scale data sets become economically feasible for most organisms, a key question for conservation biology is whether the increased resolution offered by new genomic approaches justifies repeating earlier studies based on traditional markers, rather than investing those same time and monetary resources in less-known species. Genomic studies offer clear advantages when the objective is to identify adaptive loci that may be critical to conservation policy-makers. However, the answer is far less certain for the population and landscape studies based on neutral loci that dominate the conservation genetics research agenda. We used Restriction-site Associated DNA sequencing (RADseq) to revisit earlier molecular studies of the IUCN Critically Endangered Magdalena River turtle (Podocnemis lewyana), documenting the conservation insights gained by increasing the number of neutral markers by several orders of magnitude. Earlier research indicated that P. lewyana has the lowest genetic diversity known for any chelonian, and little or no population differentiation among independent rivers. In contrast, the RADseq data revealed discrete population structure with isolation-by-distance within river segments and identified precise population breaks clearly delineating management units. It also confirmed that the species does not have extremely low heterozygosity and that effective population sizes are probably sufficient to maintain long-term evolutionary potential. Contrary to earlier inferences from more limited population genetic markers, our genomic data suggest that management strategies should shift from active genetic rescue to more passive protection without extreme interventions. We conclude with a list of examples of conservation studies in other vertebrates indicating that for many systems a genomic update is worth the investment.
Assembling Atrocity Archives for Syria
Authors: Burgis-Kasthala Michelle
Published: October 2021

This article provides an ethnographically informed comparative case study of the atrocity archiving work being undertaken by two entities: the not-for-profit, civil society organization, the Commission for International Justice and Accountability (CIJA), and the United Nations’ International, Impartial, and Independent Mechanism for Syria (IIIM). Insights from data collected are read alongside debates within the fields of international criminal law, transitional justice, and archival studies. The article argues that this archival work is of significance in exploring how international lawyers respond to the Syrian tragedy, how they understand the possibilities and limitations of criminal trials, as well as their ethical responsibilities in possessing so much sensitive material. The example of the Syrian atrocity archive, and the innovative technological approaches it requires, provides new ways of thinking through the relationship between evidence, custodianship, and the legitimacy of (possible future) criminal trials.
Using our newest VLT-KMOS HII galaxies and other cosmic tracers to test the Lambda cold dark matter tension
Authors: Mehrabi Ahmad , Basilakos Spyros , Tsiapi Pavlina , Plionis Manolis , Terlevich Roberto , Terlevich Elena , Gonzalez Moran Ana Luisa , Chavez Ricardo , Bresolin Fabio , Arenas David Fernandez , Telles Eduardo
Published: October 2021

We place novel constraints on the cosmokinetic parameters by using a joint analysis of the newest Very Large Telescope (VLT)-K-band Multi Object Spectrograph (KMOS) HII galaxies (HIIG) with the Type Ia Supernovae (SNIa) Pantheon sample. We combine the latter data sets in order to reconstruct, in a model-independent way, the Hubble diagram to as high redshifts as possible. Using a Gaussian process, we derive the basic cosmokinetic parameters and compare them with those of Lambda cold dark matter (ΛCDM). In the case of SNIa, we find that the extracted values of the cosmokinetic parameters are in agreement with the predictions of ΛCDM model. Combining SNIa with high-redshift tracers of the Hubble relation, namely HIIG data, we obtain consistent results with those based on ΛCDM as far as the present values of the cosmokinetic parameters are concerned, but find significant deviations in the evolution of the cosmokinetic parameters with respect to the expectations of the concordance ΛCDM model.
Forecasting seeing for the Maunakea observatories with machine learning
Authors: Cherubini Tiziana , Lyman Ryan , Businger Steven
Published: October 2021

The staff at the Maunakea Weather Center (MKWC) has provided daily forecasts of optical turbulence for the summit of Maunakea for more than 20 yr. Observational measures of optical turbulence at Maunakea with which to validate official MKWC forecasts have been available since mid-2009. This paper presents a machine-learning approach to translate the MKWC experience into a forecast of the nightly average optical turbulent state of the atmosphere. Maunakea observational and forecast data were collected to build a predictive model of the total and free atmospheric seeing for the following five nights. The motivation for this work is two-fold: to provide a tool/guidance to the MKWC forecaster and allow for a dynamic calibration of the optical turbulence algorithm implemented in the MKWC Weather Research and Forecasting (WRF) model.
Radiative energy from a reconnection region around massive black hole
Authors: Zhao Tian-Le , Kumar Rajiv
Published: October 2021

In the previous numerical study, we find the blob formation and ejection in the presence of magnetic reconnection in the environment of the hot flow of the accretion disc. Based on those encouraging results, in the present work, we calculate the energy and the spectrum of the emission in the different bands around sagittarius A* (Sgr A*). We assume the electrons in the magnetic reconnection region are non-thermal and emit synchrotron radiation. The electrons in the other region are thermal, which follows the thermal distribution, and the thermal electron emission mechanism is thermal synchrotron radiation. During the whole process of the magnetic evolution and reconnection, we find two peaks in the temporal light curve in the recently observed radio frequencies (230 and 43 GHz) and near-infrared (NIR) wavelengths (3.8 and 2.2 μm). Although the light curve of the NIR band is most prominent in a single peak. The first peak appears because of the blob in the plasma flow, which is formed due to the magnetic reconnection. The second peak appears due to the production of the non-thermal electrons with the evolution of the magnetic flux. Both peaks reach luminosity of more than 1026 erg s−1 for a single plasmoid/blob. For the NIR band, the highest luminosity can reach more than 1028 erg s−1. These luminosities can be high for the large simulation area and the stronger magnetic field with the multiple blobs. We infer that the observed flares are a group of magnetic reconnection phenomena, not a single one.
International peritoneal dialysis training practices and the risk of peritonitis
Authors: Cheetham Melissa S , Zhao Junhui , McCullough Keith , Fuller Douglas S , Cho Yeoungjee , Krishnasamy Rathika , Boudville Neil , Figueiredo Ana E , Ito Yasuhiko , Kanjanabuch Talerngsak , Perl Jeffrey , Piraino Beth M , Pisoni Ronald L , Szeto Cheuk C , Teitelbaum Isaac , Woodrow Graham , Johnson David W
Published: October 2021

Background

The effects of training practices on outcomes of patients receiving peritoneal dialysis (PD) are poorly understood and there is a lack of evidence informing best training practices. This prospective cohort study aims to describe and compare international PD training practices and their association with peritonitis.

Methods

Adult patients on PD <3 months participating in the Peritoneal Dialysis Outcomes and Practice Patterns Study (PDOPPS) were included. Training characteristics (including duration, location, nurse affiliation, modality, training of family members, use of individual/group training and use of written/oral competency assessments) were reported at patient and facility levels. The hazard ratio (HR) for time to first peritonitis was estimated using Cox models, adjusted for selected patient and facility case-mix variables.

Results

A total of 1376 PD patients from 120 facilities across seven countries were included. Training was most commonly performed at the facility (81%) by facility-affiliated nurses (87%) in a 1:1 setting (79%). In the UK, being trained by both facility and third-party nurses was associated with a reduced peritonitis risk [adjusted HR 0.31 (95% confidence interval 0.15–0.62) versus facility nurses only]. However, this training practice was utilized in only 5 of 14 UK facilities. No other training characteristics were convincingly associated with peritonitis risk.

Conclusions

There was no evidence to support that peritonitis risk was associated with when, where, how or how long PD patients are trained.
Designing More Effective Cigar Warnings: An Experiment Among Adult Cigar Smokers
Authors: Kowitt Sarah D , Jarman Kristen L , Cornacchione Ross Jennifer , Ranney Leah M , Smith Caleb A , Kistler Christine E , Lazard Allison J , Sheeran Paschal , Thrasher James F , Goldstein Adam O
Published: October 2021

Introduction

Little systematic evidence exists about the effectiveness of cigar warnings. This study examined the perceived message effectiveness (PME) of warning statements about different health consequences caused by cigars. PME is a validated self-report scale of how effectively a health message discourages smoking.

Aims and Methods

We conducted an online study from April to May 2020 with adults in the United States who used cigars in the past 30 days (n = 777). Participants were randomly assigned to view and rate PME (three items, range 1–5) for seven out of 37 text warning statements about different health consequences from cigar use. Linear mixed effects models evaluated the most effective warning characteristics (eg, type of health consequence), controlling for repeated measures and participant demographics.

Results

Analyses showed that health consequences about the cardiovascular system (B = 0.38), mouth (B = 0.40), other digestive (B = 0.45), respiratory system (B = 0.36), and early death (B = 0.36) were associated with higher PME scores than reproductive health consequences (all p values <.001). Similar results were found for these health consequences compared with addiction (all p values p < .001). We also observed that awareness of the health consequence was associated with higher PME scores (B = 0.19, p < .001) and length of the warning message (number of characters) was associated with lower PME scores (B = −0.007, p = .03). No differences were observed between cancer and noncancer health consequences (p = .27) or health consequences that used plain language versus medical jargon (p = .94).

Conclusions

Our study provides new evidence about the perceived effectiveness of different cigar health warning statements and identifies features that may strengthen statements.

Implications

Our study with cigar smokers from across the United States provides much-needed evidence concerning the perceived effectiveness of different cigar health warning statements and features that may strengthen such statements. Mandated cigar warnings in the United States could be strengthened by including health consequences that were perceived as more effective in our study (eg, early death), using health consequences that participants were aware of, and using short warning statements.
Recently Abstinent Smokers Exhibit Mood-Associated Dopamine Dysfunction in the Ventral Striatum Compared to Nonsmokers: A [11C]-(+)-PHNO PET Study
Authors: Calakos Katina C , Hillmer Ansel T , Angarita Gustavo A , Baldassarri Stephen R , Najafzadeh Soheila , Emery Paul R , Matuskey David , Huang Yiyun , Cosgrove Kelly P
Published: October 2021

Introduction

Chronic nicotine exposure desensitizes dopamine responses in animals, but it is not known if this occurs in human tobacco smokers. Deficits in dopamine function are likely to make smoking cessation difficult. We used positron emission tomography (PET) brain imaging with the dopamine D2/3 receptor agonist radioligand [11C]-(+)-PHNO to determine if abstinent smokers exhibit less amphetamine-induced dopamine release in the ventral striatum than nonsmokers, and whether this was associated with clinical correlates of smoking cessation.

Methods

Baseline [11C]-(+)-PHNO scans were acquired from smokers (n = 22, 7 female, abstinent 11 ± 9 days) and nonsmokers (n = 20, 7 female). A subset of thirty-seven participants (18 smokers) received oral amphetamine (0.5 mg/kg) three hours before a second [11C]-(+)-PHNO scan. Binding potential (BPND) (i.e., D2/3 receptor availability) was estimated at baseline and postamphetamine in the ventral striatum. Amphetamine-induced percent change in BPND was calculated to reflect dopamine release. Subjects also completed the Center for Epidemiologic Studies Depression Scale (CES-D).

Results

There were no group differences in baseline BPND. Amphetamine-induced percent change in BPND in the ventral striatum was significantly lower in abstinent smokers compared to nonsmokers (p=0.019; d=0.82). Higher CES-D scores were significantly associated with lower ventral striatal percent change in BPND for abstinent smokers (rs=-0.627, p=0.025).

Conclusions

In conclusion, abstinent smokers exhibited significantly less amphetamine-induced dopamine release in the ventral striatum than nonsmokers. In abstinent smokers, worse mood was significantly associated with less striatal dopamine release. Our findings highlight a potential neural mechanism that may underlie negative mood symptoms during early abstinence.

Implications

This study combined quantitative PET imaging and an amphetamine challenge to examine striatal dopamine function during early smoking cessation attempts. The findings demonstrate that recently abstinent tobacco smokers exhibit significant, mood-associated striatal dopamine dysfunction compared to nonsmokers. This study advances our knowledge of the neurobiology underlying early quit attempts, and bridges novel neural findings with clinically relevant symptoms of smoking cessation. These results may explain the challenge of maintaining long-term abstinence from smoking, and can lend insight into the development of treatment strategies for smoking cessation.
Kretek Cigarettes and Particulate Matter Emissions—An Aerosol Spectrometric Study on Typical Indonesian Brands Flavored With Cloves
Authors: Braun Markus , Marsidi Laura Lian , Klingelhöfer Doris , Groneberg David A
Published: October 2021

Introduction

In Indonesia, smoking is widespread. The same applies to second-hand smoke (SHS) exposure. More than 90% of Indonesian smokers smoke clove cigarettes, so-called Kreteks. They often contain more tar and nicotine than conventional cigarettes, but little is known about particulate matter (PM) emissions. In smokers’ homes, SHS is the main source of PM that is hazardous to health. This study presents data of PM emissions from Kreteks.

Methods

SHS was generated in a closed 2.88 m³ measuring chamber. PM fractions (PM10, PM2.5, PM1) of three popular Kretek brands (two king-sized and one slim-sized) in comparison to conventional cigarettes and a reference cigarette were measured by laser aerosol spectroscopy in real-time.

Results

The king-sized Kreteks reached PM10 and PM2.5 mean concentrations of up to about 3000 µg/m³, and PM1 mean concentrations of up to about 2700 µg/m³. Consequently, these Kreteks emitted up to about 100% more PM10 and PM2.5, respectively 84% more PM1 than the reference cigarette, and up to about 260% more PM10 and PM2.5, respectively 234% more PM1 than the other investigated cigarettes.

Conclusions

PM emissions from Kreteks can be very high. The Indonesian public should be informed and warned about it, considering the high prevalence of smoking and SHS exposure, in particular. The Indonesian tobacco control regulation is limited, and it is overdue to start with a serious and effective tobacco control policy. PM declaration to government authorities could be useful in smoking prevention and tobacco control.

Implications

Research data are limited for Kretek cigarettes, including data on particulate matter (PM). The clove buds in Kretek cigarettes are for flavoring the smoke and shall soften the smoke feeling. That makes them very popular in Indonesia, in particular. The declaration of PM data of Kreteks and other tobacco products can play a key role in a more effective tobacco policy to reduce consumption of tobacco products, not only in Indonesia. To provide data on PM emissions, this study ascertained PM amounts in passive smoke from Indonesian Kreteks.