bluuebunny/crossref_metadata_2025 huggingface dataset:
The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
Authors: Barlow Ida L. , Mackay Eirinn , Wheater Emily , Goel Aimee , Lim Sumi , Zimmerman Steve , Woods Ian , Prober David A. , Rihel Jason
Published: July 2023

Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene,
dreammist
(
dmist
), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed
dmist
gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na
+
,K
+
-ATPase regulator, FXYD1/Phospholemman. Disruption of either
fxyd1
or
atp1a3a
, a Na
+
,K
+
-ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since
atpa1a3a
and
dmist
mutants have elevated intracellular Na
+
levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na
+
pump function modulates neuronal excitability to maintain normal sleep behaviour.Sleep is an essential behavioral state, but the genes that regulate sleep and wake states are still being uncovered. A viral insertion screen in zebrafish identified a novel sleep mutant called
dreammist
, in which a small, highly-conserved transmembrane protein is disrupted. The discovery of
dreammist
highlights the importance of a class of small transmembrane-protein modulators of the sodium pump in setting appropriate sleep duration.
Ca2+ channel and active zone protein abundance intersects with input-specific synapse organization to shape functional synaptic diversity
Authors: Medeiros Audrey T , Gratz Scott J , Delgado Ambar , Ritt Jason T , O'Connor-Giles Kate M
Published: July 2023

Synaptic heterogeneity is a hallmark of nervous systems that enables complex and adaptable communication in neural circuits. To understand circuit function, it is thus critical to determine the factors that contribute to the functional diversity of synapses. We investigated the contributions of voltage-gated calcium channel (VGCC) abundance, spatial organization, and subunit composition to synapse diversity among and between synapses formed by two closely related Drosophila glutamatergic motor neurons with distinct neurotransmitter release probabilities (Pr). Surprisingly, VGCC levels are highly predictive of heterogeneous Pr among individual synapses of either low- or high-Pr inputs, but not between inputs. We find that the same number of VGCCs are more densely organized at high-Pr synapses, consistent with tighter VGCC-synaptic vesicle coupling. We generated endogenously tagged lines to investigate VGCC subunits in vivo and found that the α2δ–3 subunit Straightjacket along with the CAST/ELKS active zone (AZ) protein Bruchpilot, both key regulators of VGCCs, are less abundant at high-Pr inputs, yet positively correlate with Pr among synapses formed by either input. Consistently, both Straightjacket and Bruchpilot levels are dynamically increased across AZs of both inputs when neurotransmitter release is potentiated to maintain stable communication following glutamate receptor inhibition. Together, these findings suggest a model in which VGCC and AZ protein abundance intersects with input-specific spatial and molecular organization to shape the functional diversity of synapses.
Correlated signatures of social behavior in cerebellum and anterior cingulate cortex
Authors: Hur Sung Won , Safaryan Karen , Yang Long , Blair Hugh T , Masmanidis Sotiris C , Mathews Paul J , Aharoni Daniel , Golshani Peyman
Published: July 2023

The cerebellum has been implicated in the regulation of social behavior. Its influence is thought to arise from communication, via the thalamus, to forebrain regions integral in the expression of social interactions, including the anterior cingulate cortex (ACC). However, the signals encoded or the nature of the communication between the cerebellum and these brain regions is poorly understood. Here, we describe an approach that overcomes technical challenges in exploring the coordination of distant brain regions at high temporal and spatial resolution during social behavior. We developed the E-Scope, an electrophysiology-integrated miniature microscope, to synchronously measure extracellular electrical activity in the cerebellum along with calcium imaging of the ACC. This single coaxial cable device combined these data streams to provide a powerful tool to monitor the activity of distant brain regions in freely behaving animals. During social behavior, we recorded the spike timing of multiple single units in cerebellar right Crus I (RCrus I) Purkinje cells (PCs) or dentate nucleus (DN) neurons while synchronously imaging calcium transients in contralateral ACC neurons. We found that during social interactions a significant subpopulation of cerebellar PCs were robustly inhibited, while most modulated neurons in the DN were activated, and their activity was correlated with positively modulated ACC neurons. These distinctions largely disappeared when only non-social epochs were analyzed suggesting that cerebellar-cortical interactions were behaviorally specific. Our work provides new insights into the complexity of cerebellar activation and co-modulation of the ACC during social behavior and a valuable open-source tool for simultaneous, multimodal recordings in freely behaving mice.
BERT-PAGG: a Chinese relationship extraction model fusing PAGG and entity location information
Authors: Xu Bin , Li Shuai , Zhang Zhaowu , Liao Tongxin
Published: July 2023

Relationship extraction is one of the important tasks of constructing knowledge graph. In recent years, many scholars have introduced external information other than entities into relationship extraction models, which perform better than traditional relationship extraction methods. However, they ignore the importance of the relative position between entities. Considering the relative position between entity pairs and the influence of sentence level information on the performance of relationship extraction model, this article proposes a BERT-PAGG relationship extraction model. The model introduces the location information of entities, and combines the local features extracted by PAGG module with the entity vector representation output by BERT. Specifically, BERT-PAGG integrates entity location information into local features through segmented convolution neural network, uses attention mechanism to capture more effective semantic features, and finally regulates the transmission of information flow through gating mechanism. Experimental results on two open Chinese relation extraction datasets show that the proposed method achieves the best results compared with other models. At the same time, ablation experiments show that PAGG module can effectively use external information, and the introduction of this module makes the Macro-F1 value of the model increase by at least 2.82%.
Hearing Screening Alternative Using a Website-Based Application
Authors: Rahim Tety Hadiaty , Sunjaya Deni Kurniadi , Hilmanto Dany , Hasansulama Wijana , Putra Frans Zefanya
Published: July 2023

Background and Objectives: Indonesians encounter several barriers to regular functional hearing assessment. Hearing loss screening is only provided by tertiary-care hospitals that are not reachable by people in remote regions. This study aimed to develop a website-based hearing screening application that is accessible and inexpensive.Subjects andMethods: This comparative study was conducted between July and August 2022 in the Otolaryngology Clinic of Muhammadiyah Bandung Hospital with noise levels below 50 dB. The hearing screening was conducted using a website-based application (www.Screenout.id) and audiogram as the gold standard method. On ScreenOut, patients heard sounds with frequencies at 500, 1,000, 2,000, 4,000, and 8,000 Hz and sound intensity of each frequency at 35, 55, and 75 dB using earphones.Results: A total of 133 participants were enrolled in our study. ScreenOut showed high sensitivity, specificity, accuracy, positive predictive value, and negative predictive value (90.9%, 98.9%, 93.6%, 99.4%, and 84.8%, respectively). Regarding hearing threshold, a very strong correlation was found between ScreenOut and audiogram, ranging between r=0.843 and r=0.899. Aside from that, there was no significant difference in hearing threshold values between ScreenOut and audiogram.Conclusions: Many advantages of the ScreenOut were found, including low-cost, accessibility, and easy-to-use interface, making it favorably used in low–middle-income countries such as Indonesia.
E-learning Challenges Faced by the Primary School English Teachers of Taif City in Saudi Arabia
Authors: Allahbi Sara
Published: July 2023

Aims: The ultimate aim of this research project is to find out the adverse effect of COVID 19 on the learning environment in the Kingdom of Saudi Arabia (KSA). Furthermore, considering the impact of the corona virus on the education sector across the globe, especially at the primary level, this research addresses whether primary school English as foreign language (EFL) teachers faced challenges in dealing with online/virtual classes. To achieve the objectives and answer the research questions of the present study, the researcher elicited teachers’ perceptions regarding the challenges they face in the process of learning via virtual mode- the only alternative e-learning strategy available at the time of social distancing during Covid-19. The research method was quantitative, as data was collected from teachers’ questionnaires (adapted). The study relied on the primary school context of Taif region, Saudi Arabia. The study took around 7 months from conception to completion towards the last phase of the master’s level. The sample comprised 154 EFL Primary School teachers aged 25 to 35 years (104 females and 50 males) in Taif, Saudi Arabia. The adapted tool (a questionnaire) included different components such as attitude towards e-learning, readiness, challenges, infrastructure etc.

It was found that e-learning tools were more effective for more female teachers. Hence, the year of experience did not significantly affect e-learning materials with reference to two groups of teachers. The study also revealed that the age of male and female teachers does not contribute to the use of e-learning materials. Moreover, digital material exploitation is more easily done by the younger generation than the traditional teachers. The research findings are beneficial in redefining e-learning/virtual learning and redesigning compatible curricula, especially teaching strategies that would incorporate blended learning. In addition, teacher development activities will be initiated, keeping the teachers’ needs in view. Extension research at the doctoral level may be conducted to further support the findings by undertaking research with a larger sample. A tool should also be developed and employed to study online learning and associated factors, especially in the Taif region.
Effect of Amphipods (Grandierella megnae) Density on the Growth and Survival Rate of Mangrove Crab (Scylla tranquebarica)
Authors: Hasnidar  , Tamsil Andi
Published: July 2023

This study aims to determine the effect of amphipod density on the growth, survival and molting of mangrove crabs (Scylla tranquebarica).The research was conducted in the Installation of Tiger Shrimp Hatchery (ITSH), located Lawallu Village, Soppeng Riaja District, Barru Regency, South Sulawesi, from May to June 2022.  There was 45 mangrove crabs, with an average weight of 0.04 ± 0.005 g and a carapace width of 5.4 ± 0.1 mm which were obtained from local hatcheries. The maintenance container is a glass jar containing 0.5 liters of water, each jar is filled with one crablet. The treatments tested were differences in amphipods density, namely: A) 20 ind/0.5 L, B) 30 ind/0.5 L, C) 40 ind/0.5 L, feeding frequency is once a day for first 10 days and twice a day at 8:00 AM and 5:00 PM for next 20 days. Parameters observed were growth weight (GW) and growth carapace width (GC), specific growth rate-weight (SGR-W) and specific growth carapace- width (SGR-C), survival rate (SR) and number of molting (NM). Using a completely randomized design with three treatments and three replications, each replication consisted of 5 crabs. Data were analyzed for variance and Duncan's further test, physical and chemical parameters of water were analyzed descriptively. The results showed that amphipod density had an effect on GW, GC, SGR-W and SGR-C, but had no effect on SR and NM. Amphipod densities of 20 - 40 ind/0.5 L produced good survival and a large number of molting, but amphipod densities of 30 ind/0.5 L were more efficient for the growth of mangrove crabs.
A Review on Herb Utilization in Vannamei Shrimp Cultivation
Authors: Andriani Yuli , Pratama Rusky I.
Published: July 2023

Aquaculture activities play a crucial role in boosting fishery production. Among the various commodities, Vannamei shrimp (Litopenaeus vannamei) stands out as a leading choice due to its competitive pricing and the ability to conduct mass production with high stocking densities. However, shrimp farming has encountered challenges, particularly the occurrence of diseases that often lead to reduced production levels. These diseases typically arise from the shrimp's weakened condition and suboptimal rearing conditions. To address this issue, incorporating several herbal plants, such as noni fruit, ginger, and garlic, into the feed has emerged as an alternative to enhance shrimp immunity. Through an extensive literature search, optimal doses for these herbal additives were identified. Noni fruit demonstrated the best performance at a 6% dose, resulting in a feed conversion ratio (FCR) of 1.1 and a survival rate of 95%. Garlic performed well at a 5% dose, achieving an FCR of 1 and a survival rate of 98%. Similarly, ginger showed promising results at a 3% dose, with an FCR of 1.18 and a survival rate of 98%. The findings of this study emphasize that incorporating herbs into the feed can serve as a viable solution to enhance productivity in Vannamei shrimp farming.
A Simulation Based Comparative Analysis for Web Pages and Link Queries Using Web Ranking Algorithms
Authors: Choudhary Laxmi , Jain Rekha
Published: July 2023

In the realm of web information retrieval, the effectiveness of ranking algorithms plays a pivotal role in providing accurate and relevant search results. This simulation-based comparative analysis aims to explore the performance of two prominent ranking algorithms, namely PageRank and Weighted Page Ranking, in the context of web pages and link queries. By leveraging a comprehensive dataset comprising web pages and links, we conduct a meticulous simulation study to evaluate the effectiveness of these algorithms. Through iterative calculations and convergence analysis, we determine the rankings assigned to web pages based on their importance and connectivity within the web graph. The comparison is carried out using multiple evaluation metrics, including precision, recall, and mean average precision, to assess the algorithm’s performance in retrieving relevant web pages and handling link queries. The simulations provide valuable results of both PageRank and Weighted Page Ranking algorithms, shedding light on their applicability in various information retrieval scenarios. The performance of PageRank and Weighted PageRank algorithms can vary depending on the specific dataset, weighting factors and evaluation metrics used. The better algorithm in terms of results may depend on the particular goals and requirements of applications.
Bioactive Compounds and Antioxidant Capacity from Different Fruit Extracts of Aonla (Emblica officinalis Gaertn.)
Authors: Rathour Shubham Singh , Gurjar P. K. S. , Tiwari Sushma , Tripathi M. K.
Published: July 2023

Aonla (Emblica officinalis Geartn.) possess significant nutraceutical properties which is distributed throughout the semi-arid region of northern Madhya Pradesh, Rajasthan, plains of Uttar Pradesh, valley of Himalayas and tropical southern part of India. Aonla is also called as Indian Gooseberry which is nutraceutical rich and also utilized in ayurvedic preparations. The study has been done to access the nutraceutical potential of Aonla from different extracts of fruit pulp powder of a local cultivar. Different extracts of fruit pulp powder i.e., in pure methanol, 80 % ethanol and Ethanol: Ethyl Acetate: Water (4:3:3) were prepared and analyzed for their bioactive compounds and antioxidant capacities. The local cultivar contains a good amount of bioactive compounds and exhibit better antioxidant capacities. Results show that the solvent of Ethanol: Ethyl Acetate: Water in ratio of 4:3:3 showed highest quantity of bioactive compounds extraction and antioxidant activity followed by 80% Ethanol in aonla. While pure methanol was found best for extraction yield and tannin content.
Advances in Multi-Fruit and Vegetable Grading: A Comprehensive Review
Authors: Daheriya Mahendra , Verma D. K. , Kuruba Eresh Kumar , Mahajan K. C. , Joshi Neerav U.
Published: July 2023

Grading fruits and vegetables has become essential for cross-border trade. There are various methods and technologies used for grading fruits and vegetables. In India, fruit growers mainly rely on manual grading, which is costly, labour-intensive, and affected by seasonal labour shortages. Manual grading is inefficient, inconsistent, and time-consuming. Grading of fruits and vegetables in ensuring the quality of produce and meeting consumer demands. The World Trade Organization (WTO) emphasizes high-quality graded products, prompting farmers to seek agricultural produce-grading machines for labour alleviation, time savings, and improved quality. Fruit grading enhances value, packaging, handling, and overall marketing. Typically, fruits are graded based on size, making them more desirable for export. Size-based grading methods include various types of graders. Weight grading is based on density and specific gravity. Market responsiveness necessitates improved grading and sorting practices to ensure quality assessment. Size variations in vegetables like potatoes and onions serve as the basis for categorization according to each country's market requirements.
An Economic Analysis the Cost and Return per Hectare and Output Input Ratio of Different Size Farm of Group of Aloe Vera Cultivators in Jhunjhunu District of Rajasthan, India
Authors: Saini Harshit , Kumar Sanjay , Rai A. K.
Published: July 2023

This study presents a comprehensive cost and returns analysis of Aloe Vera cultivation, aiming to provide farmers and potential investors with valuable insights into the financial aspects of cultivating Aloe Vera. The study was conducted in Chirawa block of Jhunjhunu during the year 2022 as based on the fact that a large area is covered under the cultivation of Aloe Vera in Rajasthan. The present study was done by taking 80 farmers from 12 villages. The findings of this research provide an in-depth understanding of the financial viability of Aloe Vera cultivation. The total cultivation cost per hectare of marginal was rupee 97975, small (rupee 95596), semi medium (rupee 93147) and medium (rupee 90596).The analysis of the cost and return per hectare and farm profitability among different sizes of farms in a group of Aloe Vera cultivators indicated that medium farms generally exhibit higher profitability and efficiency. Input output ratio was 2.39, 2.50, 2.61 and 2.75 for marginal, small, semi medium and medium farmers. The cost and return analysis, along with the assessment of farm profitability, are essential tools for farmers, investors, and policymakers involved in Aloe Vera cultivation.
Coronavirus Disease 2019 – Dengue Fever Coinfection: A Case Report
Authors: Kamarany Mohammed Amood Al , Majam Ahmed , Suhail Khaled , Zuhairy Abdullah , Alabsi Elham
Published: July 2023

A lot of coronavirus disease 2019 (COVID-19) related deaths were reported in Yemen about what risk factors contributing to this excess death? Hodeidah governorate at Western part of Yemen exposed to COVID-19 pandemic like other governorates in Yemen, adding to the current chronic diseases’ problems and endemic of vector – borne diseases namely malaria and dengue. The aim of this case report is to explore more data about COVID-19 – dengue fever coinfection related deaths in Hodeidah, Yemen, with both infections cause high mortality rates. The case reported here shows a  25 ‐year‐old male, cough, hypoxia, fever 38°C, difficult in breathing, and bleeding from nose as major of symptoms with oxygen saturation (O2) of 70%, 30 ipm of respiratory rate (RR), 120/80 mmHg of blood pressure, and 120 bpm of heart rate (HR). The patient was diagnosed with bilateral asymmetrical consolidative change with ground glass opacification based X–ray, the  leukocytosis, lymphopenia, neutrophilia, and thrombocytopenia were reported. The level of random blood sugar (RBS) was normal, increase in liver enzyme was observed. Mild increase in serum creatinine with C – reactive protein (CRP) was high reactivity with positive COVID-19 and hemorrhagic dengue fever. The patient passed away (death) within two hours in triage. In conclusion , the co-infections with other infection like dengue is of high concern and this is the first reported case of COVID-19 and dengue coinfection presented as stroke and highlights the complex context of diagnostic and therapeutic management in tropical settings such as Hodeidah, Yemen.
Response of Different Combination Levels of NPK and FYM on Physicochemical Properties of Inceptisol Soil at Prayagraj District, Uttar Pradesh, India under Cultivation of Carrot (Daucus carota)
Authors: Kumar Nitish , Bharose Ram , Thomas Tarence
Published: July 2023

An experiment was conducted to investigate the Response of different combination level of NPK and FYM on physicochemical properties of Inceptisol soil at Prayagraj district, Uttar Pradesh, India under cultivation of carrot (Daucus carota). during the Rabi season of 2022-2023 at the Research Farm of the Department of Soil Science and Agricultural Chemistry, NAI, SHUATS, Prayagraj. The experimental having two factors with three levels of NPK at 0%, 50% and 100% ha-1, and three levels of FYM at 0%, 50% and 100% ha-1 which was layout in randomized block design (RBD). The results showed that the physicochemical properties of soil were significantly affected with the application of FYM in conjunction with NPK fertilizers. This was evident through a significant increase in organic carbon percentage, per cent pore space, and higher levels of available nitrogen, phosphorus, and potassium in the soil. Additionally, the combination of NPK and FYM led to a decrease in bulk density, particle density, and pH of soil. In conclusion, the experiment demonstrated that the combination of NPK and FYM positively influenced the soil health under carrot cultivation.
Ludwig's Angina with Evolution to Necrotizing Mediastinitis and Aorta Rupture Following to Death in a 23 Years-Old Woman
Authors: Fabri Cecy de Fátima Amiti , Vieira Sávio Luquetti Silva , Tinoco Paulo , Tinoco Marina Bandoli de Oliveira , Rezende Júlia Abreu , Guimarães Thayane de Matos , Silva Fábio França Vieira e , Freire Louise Mancen , Filho Renato Mataveli Ferreira , Norberg Antonio Neres
Published: July 2023

Ludwig's Angina is a rapidly progressing acute infectious process affecting the submandibular, sublingual, and submental spaces. If not promptly treated, it can be life-threatening and lead to sepsis and airway obstruction. Early detection, immediate and precise intervention including drainage, elimination of the causative factor, airway management, and antibiotic therapy are crucial for successful treatment. This manuscript presents a clinical case of Ludwig's Angina that evolved to mediastinitis and aorta rupture, resulting in a decease of a 23 years-old woman, despite treatment efforts.
Comparative Assessment of Biodegradability Potential of Pseudomonas putida and Bacillus amyloliquefaciens on Oil Spill Dispersant (Aquabreak and Teepol) in Freshwater
Authors: Mba Y. E. , Ugboma C. J. , Nrior R. R.
Published: July 2023

To compare and assessment of Biodegradability Potential of Pseudomonas putida and Bacillus amyloliquefaciens on oil spill dispersant (Aquabreak and Teepol) in Freshwater. Fresh water sample were collected from Biara, Gokana L.G.A, and were transported to the Microbiology Laboratory of Rivers State University, Port Harcourt, Nigeria for analyses while Oil spill dispersant (OSD/Aquabreak) was purchased from Barker and Hughes, all in Rivers state. Nine experimental set up were carried out using Bacillus and Pseudomonas species as the bio-augmenting organism. Controls were made without organisms. Its bioremediation potential on the pollutants and two types of test organisms  were monitored for 28 days at an interval of 7day period. The setup was aerated twice a week to provide more oxygen for the organisms to thrive. Analysis of samples were carried out using standard analytical procedures. The resultsphysiochemical property of the water shows that as follows: pH 6.5, Temperature 30.0 ºC,  Electric  conductivity 15 µs/cm, Total dissolved solid  7 mg/l ,Chlorine 0.1 mg/l, Bromine 0.2 mg/l, Salinity (0.01 mg/l), Dissolved oxygen 1.5 mg/l , Biological Oxygen Demand 0.3 mg/l, Nitrate 0.01 mg/l, Sulphate 4.18 mg/l Phosphate 0.10 to 1.5mg/l, Total Hydrocarbon content 24mg/l. Percentage (%) Ultimate biodegradability of the two oil spill dispersant OSD/teepol and OSD/aquabreak  revealed that control set-up recorded the 79.3 and 86.7 % , bioremediation set-up augmented with Bacillus amyloliquefaciens recorded 93.7 and 99.1% while the set-up augmented with P. putida had 98.0 and 94.7% respectively . It was observed  that P. putida degrade Teepol than Bacillus amyloliquefaciens while aquabreak is more degraded by Bacillus amyloliquefaciens than P. putida. Nevertheless both dispersant shown high level of degradability by the test organisms. Therefore It is recommended that oil companies and government parastatals carrying out remediation in the Niger Delta should be encouraged and OSD/Teepol and OSD/aqubreak due to their high biodegradation potential.
Synthesis, Structural Characterization, Hirschfeld Surface Analysis and Photocatalytic CO2 Reduction of Yb(III) Complex with 4-Aacetylphenoxyacetic Acid and 1,10-Phenanthroline Ligands
Authors: Tai Xi-Shi , Wang Yuan-Fang , Wang Li-Hua , Yan Xi-Hai
Published: July 2023

A new nine-coordinated Yb(III) complex [YbL3(Phen)] (1) (HL = 2-(4-acetylphenoxy)acetic acid (APA), Phen = 1,10-phenanthroline) was synthesized with 2-(4-acetylphenoxy)acetic acid, YbCl3.6H2O, NaOH and 1,10-phenanthroline as materials. Complex (1) was characterized by IR and X-ray single-crystal diffraction analysis. The results show that the Yb(III) ion is nine-coordinated with seven carboxylate oxygen atoms from five different deprotonated APA ligands and two nitrogen atoms from one Phen ligand. Complex (1) forms a 3D supramolecular framework through the - stacking interactions between the Phen aromatic rings. The hirschfeld surface analysis of complex (1) was also calculated. The CO2 photoconversion activity of complex (1) has been performed under UV-vis light irradiation. The complex (1) shows good photocatalytic activity with the yield of CO is closed to 146 mmol/g after three hours of UV-vis light irradiation, the CO selectivity has reached to 97.9%. Copyright © 2023 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).
Backmatter (Publication Ethics, Copyright Transfer Agreement for Publishing Form)
Authors: Istadi Istadi
Published: July 2023

Backmatter (Publication Ethics, Copyright Transfer Agreement for Publishing Form)
Scientific evidence in advanced care nursing consultation and the use of standardized terminologies / Evidências científicas na consulta de enfermagem em cuidado avançado e o emprego das terminologias padronizadas
Authors: Nogueira Letícia Waldomiro , Pereira Luana Dos Santos Dotta , Braga Cristiane Giffoni , Da Costa Ana Caroline , Vilella Débora Vitória Alexandrina Lisboa
Published: July 2023

Objetivo: identificar, pelas evidências científicas na consulta do enfermeiro em cuidado avançado, o emprego das terminologias padronizadas nas etapas do processo de enfermagem em contextos de cuidado. Método: revisão integrativa realizada em seis etapas. Resultados: foram selecionados 14 artigos. As terminologias padronizadas mais utilizadas pelo enfermeiro durante as consultas de enfermagem foram a CIPE® (65%), seguida da NNN- NANDA-I, NOC e NIC (21%); NOC (7%); CIPE®, NNN e Omaha System (7%). 43% utilizam a linguagem padronizada nas etapas de diagnóstico, resultado e intervenção. A respeito do contexto de cuidado, o uso de terminologias padronizadas de enfermagem era empregado 36% em ambiente hospitalar, 14% em Home Care, 14% em Atenção Primária à Saúde, 7% em clínica privada e 29% não foram informados. Conclusão: a terminologia padronizada mais utilizada foi a CIPE® (65%), nas etapas de diagnóstico, resultado e intervenção (43%) no ambiente hospitalar (36%).
TRPM4 contributes to cell death in prostate cancer tumor spheroids, and to extravasation and metastasis in a zebrafish xenograft model system
Authors: Bochen Florian , Subedi Saurav , La Manna Federico , Jarrin Sofia , Papapostolou Irida , Kruithof‐de Julio Marianna , Peinelt Christine
Published: January 2025

Transient receptor potential melastatin‐4 (TRPM4) ion channel expression is upregulated in prostate cancer (PCa), contributing to increased cell proliferation, migration, adhesion, epithelial‐to‐mesenchymal transition, cell cycle shift, and alterations of intracellular Ca2+ signaling. GEO2R platform analysis of messenger RNA (mRNA) expression of ~ 6350 genes in normal and malignant prostate tissue samples from 15 PCa patients demonstrates that TRPM4 expression is upregulated sixfold and is among the most significantly upregulated genes in PCa. We find that absence of TRPM4 reduced PCa tumor spheroid size and decreased PCa tumor spheroid outgrowth. In addition, lack of TRPM4 increased cell death in PCa tumor spheroids, a phenotype that is absent in two‐dimensional (2D) cancer cell systems. Lastly, absence of TRPM4 in PCa cells reduced extravasation and metastatic burden in a preclinical zebrafish cancer model. Taken together, our findings show that TRPM4 is an attractive therapeutic target in PCa and highlights the need for future development of pharmacological tools.
NIDA director touts prevention, treatment access
Authors: Knopf Alison
Published: January 2025

In her blog this month, Nora Volkow, M.D., director of the National Institute on Drug Abuse (NIDA), focused on the successes in the field commemorating the agency's 50th year, but also on the challenges currently faced.
Functional Modification of Dawson‐Type Arsenomolybdate for Enhanced Ultracapacitor Performance and Nitrate‐to‐Ammonia Production
Authors: Shi Bing‐Xue , Wang Yu‐Wen , Wang Mei‐Lin , Cui Li‐Ping , Yu Kai
Published: January 2025

Polyoxometalates (POMs) are promising electrocatalysts and pseudo‐capacitive materials due to their reversible multi‐electron redox properties. In this study, Dawson‐type mono‐arsenic‐capped arsenomolybdate are anchored into channels of {Cu(trz)2}7 metal–organic network yielding a solution‐stable host‐guest structure, [{CuI(trz)2}7{AsIIIAsV2MoV4MoVI14O62}]2·3H2O (2), which exhibits higher conductivity and specific capacity, excellent rate performance and cycle stability than (biz)9(Hbiz)3{AsIII1.5AsV2Mo18O62}2·2H2O (1) and most reported POMs, ascribing to the excellent Faraday properties of POMs, metal–organic conductive network, and the advantages of host‐guest structure in surface area and stability. The AC//2‐CPE device demonstrates energy density and power density of 25.45 Wh kg−1 and 1991.53 W kg−1, and 92.4% capacity retention after 10 000 cycles. Moreover, compound 2 as nitrate reduction reaction (NO₃RR) electrocatalyst achieves a current density of 150 mA cm−2 at −0.5 V, ammonia production rate of 15.28 mg h−1 cm−2, and Faradaic efficiency of up to 90%. Density functional theory is employed to thoroughly investigate the adsorption active sites and the detailed energetic steps corresponding to the overall reaction pathway of NO3RR regulated by compound 2. This study reveals that encapsulating POMs clusters into a metal–organic network can increase the redox active sites, improve stability, and conductivity, thereby enhancing the energy storage and catalytic activity of POMs at the molecular level.
A Paper‐Based Sensor for the Detection of Gastroesophageal Reflux Disease Utilizing a Cleavable Fluorescent Polymer
Authors: He Zejian , Wu Jun , He Zhen , Chen Jia , Fang Weipin , Zhang Yifan , Shen Xinyi , Ren Yangjing , Chen Yulong , He Jianguo , Yan Donghai , Chen Pin , Zhou Mi
Published: January 2025

Nowadays, gastroesophageal reflux disease (GERD) has emerged as one of the major hazards to the health of the upper gastrointestinal tract, and there is an urgent need for a low‐cost, user‐friendly, and non‐invasive detection method. Herein, a paper‐based sensor (CP sensor) for the non‐invasive screening of GERD is proposed. The sensor is structured as a specially shaped cellulose paper strip embedded with fluorescent colloids, which are self‐assembled from a cleavable synthetic fluorescent polymer (P4). Benefiting from the introduction of amide bonds and the unique assembled structure of the nanocolloids, the pepsin in the sample solution will hydrolyze the water‐soluble branches in the micellar shell during detection, resulting in a corresponding output of the fluorescent signal. This responsiveness, which can be observed by the naked eye, is so sensitive with a minimum detectable concentration for pepsin as low as 0.3 ng·mL−1. Clinical trials have further demonstrates that the designed paper sensor is capable of providing improved accuracy in the early diagnosis of GERD.
Engineered Age‐Mimetic Breast Cancer Models Reveal Differential Drug Responses in Young and Aged Microenvironments
Authors: Yang Jun , Hawthorne Lauren , Stack Sharon , Blagg Brian , Ali Aktar , Zorlutuna Pinar
Published: January 2025

Aging is one of the most significant risk factors for breast cancer. With the growing interest in the alterations of the aging breast tissue microenvironment, it is identified that aging is related to tumorigenesis, invasion, and drug resistance. However, current pre‐clinical disease models often neglect the impact of aging and sometimes result in worse clinical outcomes. In this study, aged animal‐generated materials are utilized to create and validate a novel age‐mimetic breast cancer model that generates an aging microenvironment for cells and alters cells toward a more invasive phenotype found in the aged environment. Furthermore, the age‐mimetic models are utilized for 3D breast cancer invasion assessment and high‐throughput screening of over 700 drugs in the FDA‐approved drug library. 36 potential effective drug targets as well as 34 potential drug targets with different drug responses in different age groups are identified, demonstrating the potential of this age‐mimetic breast cancer model for further in‐depth breast cancer studies and drug development.
Super‐Resolution Goes Viral: T4 Virus Particles as Versatile 3D‐Bio‐NanoRulers
Authors: Gallea José Ignacio , Nevskyi Oleksii , Kaźmierczak Zuzanna , Gligonov Ivan , Chen Tao , Miernikiewicz Paulina , Chizhik Anna M. , Reinkensmeier Lenny , Dąbrowska Krystyna , Bates Mark , Enderlein Jörg
Published: January 2025

In the burgeoning field of super‐resolution fluorescence microscopy, significant efforts are being dedicated to expanding its applications into the 3D domain. Various methodologies have been developed that enable isotropic resolution at the nanometer scale, facilitating the visualization of 3D subcellular structures with unprecedented clarity. Central to this progress is the need for reliable 3D structures that are biologically compatible for validating resolution capabilities. Choosing the optimal standard poses a considerable challenge, necessitating, among other attributes, precisely defined geometry and the capability for specific labeling at sub‐diffraction‐limit distances. In this context, the use of the non‐human‐infecting virus, bacteriophage T4 is introduced as an effective and straightforward bio‐ruler for 3D super‐resolution imaging. Employing DNA point accumulation for imaging in nanoscale topography (DNA‐PAINT) along with the technique of astigmatic imaging, the icosahedral capsid of the bacteriophage T4, measuring 120 nm in length and 86 nm in width, and its hollow viral tail is uncovered. This level of detail in light microscopy represents a significant advancement in T4 imaging. A simple protocol for the production and preparation of samples is further outlined. Moreover, the extensive potential of bacteriophage T4 as a multifaceted 3D bio‐ruler, proposing its application as a novel benchmark for 3D super‐resolution imaging in biological studies is explored.
Phase‐Engineered Bi‐RuO2 Single‐Atom Alloy Oxide Boosting Oxygen Evolution Electrocatalysis in Proton Exchange Membrane Water Electrolyzer
Authors: Yang Zhichao , Ding Yutian , Chen Wen , Luo Shuiping , Cao Daofan , Long Xin , Xie Lei , Zhou Xincheng , Cai Xinyi , Liu Ke , Fu Xian‐Zhu , Luo Jing‐Li
Published: January 2025

Engineering nanomaterials at single‐atomic sites can enable unprecedented catalytic properties for broad applications, yet it remains challenging to do so on RuO2‐based electrocatalysts for proton exchange membrane water electrolyzer (PEMWE). Herein, the rational design and construction of Bi‐RuO2 single‐atom alloy oxide (SAAO) are presented to boost acidic oxygen evolution reaction (OER), via phase engineering a novel hexagonal close packed (hcp) RuBi single‐atom alloy. This Bi‐RuO2 SAAO electrocatalyst exhibits a low overpotential of 192 mV and superb stability over 650 h at 10 mA cm−2, enabling a practical PEMWE that needs only 1.59 V to reach 1.0 A cm−2 under industrial conditions. Operando differential electrochemical mass spectroscopy analysis, coupled with density functional theory studies, confirmed the adsorbate‐evolving mechanism on Bi‐RuO2 SAAO and that the incorporation of Bi1 improves the activity by electronic density optimization and the stability by hindering surface Ru demetallation. This work not only introduces a new strategy to fabricate high‐performance electrocatalysts at atomic‐level, but also demonstrates their potential use in industrial electrolyzers.
Unraveling Plasmonic Field Contributions in Catalysis with Au@Al2O3‐Pd‐Au@Al2O3 Trimer Arrays
Authors: Yao Lei , Wei Yunjia , Zhao Xing , Zhu Xiangnan , Chen Dexiang , Tang Xiao , Fan Xingce , Qiu Teng , Hao Qi
Published: January 2025

Plasmonic catalysis employs nanomaterials to mediate the conversion of photon energy into localized electromagnetic fields, hot carriers, and thermal energy, facilitating molecular transformations under mild conditions. Understanding the mechanisms governing plasmonic effects is crucial for optimizing the yield and selectivity in plasmonic catalysis. However, challenges persist in elucidating the role of plasmonic field effects due to the complex interactions among various contributing mechanisms. This study introduces Au@Al2O3‐Pd‐Au@Al2O3 trimer arrays to decouple plasmonic field effects from other influences. The alumina coating on the Au particles prevents charge transfer between gold and reactants, enabling focused examination of the field effects on Pd catalysts. Employing real‐time monitoring with liquid‐state surface‐enhanced Raman scattering (SERS), this work investigates the catalytic efficiency and kinetics of the C─C coupling reactions on palladium by tuning the near‐field strengths. Polarization‐dependent studies reveal that strong near‐field intensity around the trimer structures is amplified by ≈60‐fold under optimized conditions. The findings offer insights for optimizing catalytic reaction conditions and designing more effective composite plasmonic catalysts.
Hybrid Nanoantenna‐Nanopillar Si Schottky Photodetectors for Ultrawide Wavelength‐Tunable Photodetection
Authors: Yu Yang , Li Ziyuan , Zheng Ze , Choi Duk Yong , Xu Lei , Zhang Fanlu , Vora Kaushal , Vaidya Gayatri , Jin Weiqi , Rahmani Mohsen , Hattori Haroldo T. , Tan Hark Hoe , Jagadish Chennupati , Fu Lan
Published: January 2025

Active optical antennas, a novel Schottky photodiode design, have drawn significant attention due to their advantages of near‐field enhancement and hot‐electrons generation for sub‐bandgap photodetection. However, the reported active antenna‐based Si Schottky photodetectors have shown low photoresponse in a limited wavelength range below 1650 nm. This work reports hybrid nanoantenna (NA)‐nanopillar (NP) Si Schottky photodetectors for ultrawide and wavelength‐tunable photodetection at room temperature, without using any optical filters. The designed hybrid nanostructure significantly enhances the electric dipole‐induced resonance in the NAs, leading to strong and wavelength‐tunable light absorption in a wide spectral range from 1200 to 2200 nm. Moreover, due to the strong plasmon resonance and 3D cavity effect in the hybrid nanostructure, room temperature responsivities of 0.46 A W−1 and 0.25 mA W−1, and detectivities of 2.24 × 1011 and 1.25 × 108 cm √Hz W−1 are obtained at 950 and 1700 nm, respectively. The device also features a high response speed of 7.2/7.6 ns (rise time/fall time) and 194/240 µs at 850 and 1310 nm, respectively. Such broadband, wavelength‐selective, and high‐speed hybrid NA‐NP Si Schottky photodetectors, with extended detection wavelength, are promising for Si‐based photonic systems for material identification, spectroscopy, imaging, and night vision applications.
Internalizing Psychiatric Symptoms in People With Mosaicism for Trisomy 21
Authors: Brown Ruth C. , D'Aguilar Allison , Hurshman Quinn , NailorZee Rebekah , York Timothy P. , Capone George , Amstadter Ananda B. , Jackson‐Cook Colleen
Published: January 2025

People with mosaicism for trisomy 21 have been shown to exhibit many of the same phenotypic traits present in people with non‐mosaic Down syndrome, but with varying symptom severity. However, the behavioral phenotype of people with mosaic Down syndrome (mDS) has not been well characterized. This study aimed to examine the prevalence of self‐report and caregiver‐report symptoms of depression and anxiety among a sample of 62 participants with mDS aged 12–46 and assess their association with the percentage of trisomy 21 in blood and/or buccal mucosa cells. The overall MANCOVA revealed a significant effect of trisomy on the set of internalizing scales, controlling for age and gender (p = 0.038, partial eta2 = 0.22). However, follow‐up univariate analyses showed that the initial significant effect of trisomy on fear (p = 0.049, partial eta2 = 0.08) did not survive correction for multiple comparisons (adjusted p = 0.300). No other effects were significant. This study highlights the high occurrence of depression and anxiety symptoms in individuals with mDS and the need for routine assessment to optimize their care. It also demonstrates the ability of people with mDS to complete these evaluations, thereby supporting their inclusion in research studies/clinical trials.
Role of Dimethyl Sulfoxide Concentration on Hole Transport Mechanism and Efficiency of Hybrid Solar Cells
Authors: Krishnan Akhil , Ahmed Nafis , Bhargav P. Balaji , Prakash D. Gnana , Aravinth K. , Ramasamy P.
Published: January 2025

PEDOT:PSS‐Si hybrid solar cell fabrication technique is highly cost‐effective, performed at low temperatures, and easy to fabricate compared to other solar cell fabrication techniques such as PERC, HJT, IBC, perovskite, and multijunction solar cells. In this experimental and theoretical investigation, we studied the effect of additive concentration on hybrid solar cell performance. We prepared PEDOT:PSS dispersion solutions with various weight percentages of DMSO (5 wt%, 7 wt%, 12 wt%, and 17 wt%) and 0.2 wt% of Triton X‐100. We used dimethyl sulfoxide as an additive and analyzed the variation in carrier concentration of PEDOT:PSS. Additionally, we examined the conductivity of PEDOT:PSS and the absorbance of the PEDOT:PSS layer. Hall effect measurement was used to determine the carrier concentration of PEDOT:PSS and Si. AFM analysis helped in understanding the polymer structure, distribution, and domain formation. We fabricated PEDOT:PSS‐Si hybrid cells using different weight percentages of DMSO. Solar cells with 7 wt% and 5 wt% of DMSO yielded 7.26% and 7.31% of power conversion efficiency, respectively. Numerical simulation was conducted using SCAPS 1D, and the obtained results aligned well with the experimental outcomes.
Structure and Properties of Poly(Butylene Adipate‐Co‐Terephthalate)/Soybean Isolate Protein Composites With a Special Interface Structure
Authors: Yi Yazhou , Jian Kui , Zhang Yaqing , Wei Ningning , Ren Mingtian , Wu Qiangxian
Published: January 2025

It will significantly reduce carbon dioxide emissions by incorporating natural polymers into eco‐friendly composites. Improving compatibility in eco‐friendly composites is essential for enhancing their mechanical properties for broad applications. In this study, we synthesized a unique structured polyurethane prepolymer (PBAPU) and utilized it as a compatibilizer. We then mixed varying amounts of PBAPU with PBAT and SPI to produce a series of PBAT/SPI composites and subsequently investigated their structure and properties. The PBAT/SPI composites, with the addition of 15% compatibilizer, saw their tensile elongation increase by 207% and their impact strength improve by 236%, showing that the addition of PBAPU enhanced the mechanical properties of the composites. Furthermore, the scanning electron microscopy images revealed a reduction in cracks and gaps at the PBAT/SPI interface, with a more uniform dispersion, indicating a significant enhancement in the compatibility of the composites. The strong urethane linkage interactions between the PBAPU interfacial layer and SPI, as well as the crystalline PBA‐PBA interactions between the PBAPU interfacial layer and the PBAT matrix, are the reasons for the improved compatibility of the PBAT/SPI composites. Therefore, incorporating PBAPU with a special structure enhances the compatibility and performance of the PBAT/SPI composites.