bluuebunny/crossref_metadata_2025 huggingface dataset:
Mengurai Perempuan dan Pembangunan (Studi Kasus Kartini Kendeng)
Authors: Rajagukguk Samuel B. T.
Published: September 2022

Sudah hampir 3 (tiga) tahun, Indonesia khususnya masyarakat Pegununan Kendeng di Jawa Tengah dihadapkan pada persoalan bagaimana sebenarnya konsep yang tepat dalam merumuskan masyarakat yang dicita-citakan. Tidak lebih perdebatannya adalah siapa yang akan merumuskan ataupun mendeskripsikan masyarakat ideal. Hal ini dihadapkan pula oleh kekuasaan dan sifat mendasar dari manusia Indonesia itu sendiri. Definisi pembangunan guna mencapai masyarakat yang dicitakan menjadi program sentral pemilik modal, kuasa, dan budaya-budaya Patriarki manusia Indonesia. Masyarakat kecil termajinalkan, masyarakat adat dihilangkan, kaum minoritas dianggap tidak ada, hingga persoalan lainnya. Tebakannya adalah mungkin saja Indonesia masih proses menjadi. Tetapi, nilai fundamentalnya adalah menjadi apa, konsep kaum urbankah, pandangan kaum andropsentriskah, atau lainnya untuk mencapai masyarakat yang inginkan. Bidang pertambangan dan industrialisasi sering kali yang menjadi korban adalah perempuan. Apakah dalam pengetahuan perempuan menolak pertambangan dan/atau industrialisasi.Kata Kunci: Pembangunan, Pertambangan, Industrialisasi, Perempuan.
Hyperlipidemic pancreatitis in children: characteristics of the course and a clinical case presentation
Authors: Lembryk I. S. , Tsytsiura O. O. , Zhyliak O. V. , Dutchuk O. V. , Buiak P. Z. , Dutchak Н. M. , Krasivskyi I. Y.
Published: September 2022

The article presents data on the prevalence, clinical, diagnostic and treatment characteristics of acute hyperlipidemic pancreatitis in children, based on a literature review and a clinical case presentation.
The aim of this work is to define the nature of hyperlipidemic pancreatitis in children based on the literature review, and the clinical case presentation.
Material and methods. The analysis of the literature data of the last 10 years through the Google Scholar and PubMed databases was performed. The clinical course, blood tests assessing protein and lipid indicators, abdominal ultrasound findings typical for acute hyperlipidemic pancreatitis were examined in a patient O. S., born in 2007.
Results. The disease was diagnosis based on the study of anamnestic, clinical, laboratory and ultrasound findings. Lipid profile test revealed an increase in the level of cholesterol and triglycerides, and a decrease in the level of high-density lipoproteins. Abdominal ultrasound showed changes in the pancreatic tissues: the pancreas was significantly enlarged with a heterogeneous structure of the parenchyma, the echogenicity was significantly increased due to edema, and there was a small amount of effusion. Laparoscopic drainage of the omentum and abdominal cavity was recommended and performed. The key to the successful treatment was repeated long-term use of detoxification and hypolipidemic therapy in combination with dietary corrections (a low-fat diet).
Conclusions. As of today, acute hyperlipidemic pancreatitis continues to be one of the least studied and rare pathologies among the pediatric population. This necessitates the search for and implementation of both diagnostic and treatment algorithms into clinical practice to timely correct and prevent complications.
Comparative analysis of the effectiveness of thermal methods for the treatment of varicose veins of the lower extremities
Authors: Atamaniuk O. Yu. , Skrypko V. D. , Atamaniuk V. M.
Published: September 2022

The aim of the work is to analyze the effectiveness and safety of thermal methods (endovenous laser coagulation (EVLC) and radiofrequency ablation (RFA)) for the treatment of varicose veins of the lower extremities and the impact on the quality of patient life.
Materials and methods. An open, prospective study of the comparative efficacy of EVLC (124 patients) and RFA (112 patients) for the treatment of patients with varicose vein disease was conducted. Both groups were compared by age, sex, body mass index, clinical manifestations of the venous pathology severity and assessed the risk of potential complications. Determination of quality of life criteria was performed using the AVVQ questionnaire.
Results. The average number of cycles performed for the RFA procedure was 13.8 (7; 14), and the energy was 60.1 (8; 80) J/cm for EVLC. The average value of the VAS during the first 14 days after treatment in the EVLC group was 2.2 (sd 1.9), RFA – 0.8 (sd 0.9), P > 0.05. The majority of patients who underwent EVLC used painkillers – 82 (66.1 %), RFA – 48 (42.9 %), P < 0.001. The results of duplex monitoring in the first 48 hours recorded 100 % occlusion of the treated segments of the great saphenous vein in both study groups; after 1 and 12 months – in 98.4 % (96.0 %) of patients after EVLC and in 100.0 % (98.2 %) – after RFA, respectively. One or more adverse events during the first 2 weeks after treatment were reported by 86 patients (69.4 %) in EVLC group, 52 (46.4 %) – in RFA group. The differences between EVLC and RFA were statistically significant (P < 0.001).
VCSS and AVVQ values were significantly decreased one month after treatment, continuing the positive trend for up to 12 months. However, no significant difference between ablation methods was observed (P > 0.05).
Conclusions. Endovenous laser coagulation and radiofrequency ablation are safe and effective thermal treatments for varicose veins of the lower extremities, which lead to a statistically significant improvement in the quality of patient life. Radiofrequency ablation has the advantage of a lower risk of developing general complications.
Perception of operational environment conditions and situations as a prediction of adaptive potential in servicemen
Authors: Kalnysh V. V. , Shvets A. V. , Maltsev O. V. , Sereda I. K. , Kikh A. Yu. , Marushchenko K. Yu.
Published: September 2022

The aim of the study is to define the adaptive potential of servicemen participating in warfare based on the formation of their perceptions related to operational environment conditions and characteristics of heart rate variability (HRV).
Materials and methods. 70 combatants – men aged 22–30 years were examined. According to a specially designed questionnaire that reflected the issue of combat and deployment life factors among servicemen in the operational environment, an assessment of their perceptions related to the impact of internal and external factors was conducted. The HRV parameters of the studied servicemen were determined by standard methods using computer rhythmograph. Statistical analysis of the data was performed using the methods of parametric (Student’s t-test) and non-parametric statistics (Wilcoxon’s T-test), cluster, step-by-step multiple correlation and step-by-step discriminant analysis using software package Statistica 13.3.
Results. The studied contingent was divided into two “hypersusceptible” and “hypоsusceptible” groups based on the use of their perceptions related to the operational environment factors. It has been noted that group 1 had greater values of median profiles than group 2. The differentiation of the studied contingent into groups of “hypersusceptible” and “hyposusceptible” to harmful operational environment factors was more noticeable when applying criteria of deployment life factors. HRV values (LFnorm, HFnorm and LF/HF) differed significantly in both examined groups. It has been found that the multiple correlation coefficient value for group 1 was R = 0.61 (P < 0.007), and for group 2 – R = 0.83 (P < 0.00009), indicating the presence of a stronger link between the level of adaptability and the parameters of heart rate regulation among “hyposusceptible” servicemen. The developed decisive rules allow identifying the adaptive potential level in servicemen by assessing the effects of combat factors and deployment life.
Conclusions. Among the operational environment factors, the deployment factors have been found to be the most significant, suggesting that these characteristics were the most susceptible to harmful operational environment factors in combatants. The analysis of the HRV characteristics in servicemen with “hypersusceptibility” and “hyposusceptibility” to the operational environment factors has shown the higher adaptive potential in representatives of “hyposusceptible” serviceman group. The decision support model for individual assessment of the adaptive potential of servicemen according to the parameters of their perceptions of the operational environment factors (combat and deployment life factors) has been developed.
The study of the optical activity of some S-derivatives 4-R-5-((((3-(pyridin-4-yl)-1H-1,2,4-triazole-5-yl)thio)methyl)-4H-1, 2,4-triazole-3-thiols
Authors: Karpun Ye. O. , Borsuk S. O. , Kucherenko L. I. , Parchenko V. V.
Published: September 2022

About half of the drugs currently produced are chiral compounds, and about 90 % of these compounds are sold as racemates, consisting of an equimolar mixture of two enantiomers. Although they have the same chemical structure, most of the optical isomers of chiral substances show marked differences in biological activity. It is known that the presence of a single asymmetric atom has become almost an integral part of advanced drug design.
The aim of this work was to determine the angle of rotation of the polarization plane of solutions of some S-derivatives of 4-R-5-((((3-(pyridin-4-yl)-1H-1,2,4-triazole-5-yl)thio)methyl))-4H-1,2,4-triazole-3-thiols and the establishment of regularities between the structure of the studied molecules and their optical activity.
Materials and methods. The subject of the study was 2-[5-R1-4R2-1,2,4-triazole-3-ylthio]-1-aryletanols. The study of the angle of rotation of the plane of polarization of solutions of newly synthesized compounds was carried out using an Atago AP-300 polarimeter and the DFU 2.2.7 physical-chemical analysis method “Optical rotation”.
Results. The results of the physical-chemical analysis were carried out that the studied compounds exhibit optical activity. The compound 1-((4-methyl-5-(((3-(pyridin-4-yl)-1H-1,2,4-triazole-5-yl)thio)methyl)-4H-1,2,4-triazole-3-yl)thio)-2-phenylethan-1-ol (+43° [deg∙g/cm3∙dm]). The only levorotatory substance was 1-(4-fluorophenyl)-2-((4-methyl-5-(((3-(pyridin-4-yl)-1H-1,2,4-triazole-5-yl)thio))methyl)-4H-1,2,4-triazole-3-yl)thio)ethan-1-ol with specific rotation [α]D20 = -43° [deg∙g/cm3∙dm].
Conclusions. Studies had shown that 1-(4-fluorophenyl)-2-((4-methyl-5-(((3-(pyridin-4-yl)-1H-1,2,4-triazole-5-yl))thio)methyl)-4H-1,2,4-triazole-3-yl)thio)ethan-1-ol was able to rotate the light polarization plane to the left, which was evidence of the advantage of the S-enantiomer in the racemic mixture, and therefore this compound was considerable interest for further preclinical research. Also, all other analyzed compounds behave as optical isomers.
Determination of riboflavin in dragee “Revit” by HPLC
Authors: Varynskyi B. О. , Zeleniuk M. Yu. , Kaplaushenko A. H.
Published: September 2022

The aim of the work is development of express, selective, reproducible methods for the determination of riboflavin by HPLC in a multivitamin preparation, dragee “Revit”.
Materials and methods. Degasser, binary pump, autosampler, thermostated column compartment, diode array detector. Chromatographic column Zorbax SB-C18, 30 mm × 4.6 mm, 1.8 µm. Samples were prepared using analytical electronic scales Kern ABT 100-5M, ultrasonic bath Ultrasonic XUBA 3, nylon ultrafilters 0.12 µm.
Results. 0.1 % formic acid was selected as a buffer solution in the eluent. The maximum values of logD were observed at intervals for riboflavin at pH from 2 to 4. Therefore, the maximum retention of the riboflavin was observed when using the eluent, рН which corresponds to this interval. The pH value for 0.1 % formic acid solution was 2.7. Therefore, such a buffer was chosen by us as part of the eluent. Acetonitrile was selected as the organic modifier. The optimal content of the compound was observed at 10 % acetonitrile.
Conclusions. The chromatographic behavior of riboflavin on the basis of chemical-analytical properties was studied. The optimal conditions for chromatographic determination were determined and a specific, express method for the determination of riboflavin in “Revit” dragee was developed. The technique on a real sample of “Revit” dragee was elaborated. The method was recommended for use in control and analytical and research laboratories.
Synthesis and properties of some 3-(5-(4-methoxyphenyl)pyrazol-3-yl)-6-R-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles
Authors: Fedotov S. O. , Hotsulia A. S. , Panasenko O. I.
Published: September 2022

Pyrazole and 1,2,4-triazole derivatives play an important strategic role in modern medicine and pharmacy. This fact is due to the significant possibilities of chemical modification and significant pharmacological potential among the derivatives of these heterocycles. The introduction of 1,2,4-triazole and pyrazole fragments into the structure of new substances allows to influence the formation of a certain type of activity. The structural combination of these heterocycles in one molecule increases the likelihood of interaction with various biological targets. At the same time, the creation of condensed systems involving 1,2,4-triazole is undoubtedly scientifically attractive and promising.
The aim of the research was to study the conditions for obtaining 3-(5-(4-methoxyphenyl)pyrazol-3-yl)-6-R-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles and studying the properties of these compounds.
Materials and methods. The first stage of the synthetic part of the work involved the use of diethyl oxalate and 1-(4-methoxyphenyl)ethan-1-one with the participation of sodium hydride in toluene. The obtained ethyl 4-hydroxy-4-(4-methoxyphenyl)-2-oxobut-3-ethanoate in the next step was used in the process of conversion into ethyl 5-(4-methoxyphenyl)pyrazole-3-carboxylate with the participation of hydrazine hydrate. Further modification of the molecule was the stepwise formation of the structure of 4-amino-5-(5-(4-methoxyphenyl)pyrazol-3-yl)-1,2,4-triazole-3-thiol. The next stage of the work involved the interaction with carboxylic acids in the environment of phosphorus oxychloride. To determine the composition and identify the structure of the isolated substances, 1H NMR and infrared spectra were recorded, as well as qualitative and quantitative indicators of the elemental composition of the synthesized structures were obtained. The individual nature of the presence of substances and the degree of their purity were determined using high performance liquid chromatography.
Results. Synthesis of 3-(5-(4-methoxyphenyl)pyrazol-3-yl)-6-R-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles was performed and optimal conditions were determined the process of obtaining these substances. The structure of the products of chemical transformation was confirmed and the results of the study of physical properties were recorded. The results of docking studies allowed to confirm the prospects of the chosen direction of synthetic transformations, which ultimately allowed to determine the biological potential of the obtained compounds. The model enzyme was 14-α-demethylase lanosterol (code 3LD6), information on which was used from the database of the Protein Structures Database (PDB).
Conclusions. As a result of the molecular docking, data were obtained that form an idea of a certain level of probability of the effect of synthesized compounds on the activity of 14α-demethylase lanosterol, which justified the need for further study of antifungal activity.
Value of P-selectin in the prognosis of cardiovascular events in patients with multiple myeloma
Authors: Panasenko M. O. , Samura B. B. , Dotsenko S. Ya.
Published: September 2022

Multiple myeloma (MM) survival has greatly improved in recent decades. MM is usually diagnosed in elderlies. Patients do not necessarily die from MM, so cardiovascular health may be a key factor threatening long-term survival.
The aim of the research: to evaluate the prognostic value of circulating P-selectin for cardiovascular events in patients with multiple myeloma in full or partial remission.
Materials and methods. 97 patients with MM with complete or partial remission were enrolled in the study. None of the patients had received any lipid-modulating medications, including statins or fibrates, before enrollment. The observation period was up to 1 year. Blood samples for biomarkers measurements were collected. ELISA method for measurements of circulating level of P-selectin was used.
Results. During the observation period progression of MM was proved in 25 patients, 5 persons were excluded for poor follow-up. 67 patients were included in the statistical analysis. Thirty-six cumulative clinical events occurred in 18 patients (26.9 %) within the follow-up, with their distribution being as follows: 2 cardiovascular deaths, 16 cardiac arrhythmias, 3 cardiac ischemic events, 1 stroke, 4 chronic heart failures, and 10 hospital admissions for cardiovascular reasons. 2 deaths were not related with cardiovascular pathology. Medians of circulating levels of P-selectin in free-events subject cohort and subjects cohort with cardiovascular events were 58.24 ng/ml (95 % confidence interval (CI) = 32.27–77.48 ng/ml) and 87.57 ng/ml (95 % CI = 51.12–114.89 ng/ml) (Р < 0.01). In multivariate logistic regression circulating P-selectin independently predicted cumulative cardiovascular events (odds ratio (OR) = 1.11; 95 % CI = 1.08–1.15; Р = 0.02) in patients after sever coronavirus disease (COVID-19) within 1 year of observation period.
Conclusions. Among patients with documented multiple myeloma in remission increased circulating P-selectin was associated with increased cumulative cardiovascular events.
Folktales about The Tatras in children’s literature
Authors: Dzuriaková Jana
Published: September 2022

In my contribution I will discuss the folktales about the Tatras and their reference in children’s literature. I will focus mainly on the stories that appear in the textbook of Slovak literature for the 5th year of elementary schools. Specifically, the tale of Tatranská Kikimora, but also others are discussed. Through this and other individual stories, pupils can learn and get to know more about the environment in which they live.
Human trafficking and the African woman: A critical study of Darko’s Beyond the horizon and Adimora-Ezeigbo’s Trafficked
Authors: Nutsukpo Margaret Fafa
Published: September 2022

Globalization, gender inequality and poverty render African women vulnerable to trafficking; this is explored by Darko and Adimora-Ezeigbo in their novels. From the feminist perspective, this article analyzes the authors’ portrayal of trafficking, factors and structures that sustain it, and the significance of the construction of self-narratives during victim’s rehabilitation. It also proffers preventive strategies and effective avenues for victims’ rehabilitation and reintegration into society.
Logistic damping effect in chemotaxis models with density-suppressed motility
Authors: Lyu Wenbin , Wang Zhi-An
Published: September 2022

This paper is concerned with a parabolic-elliptic chemotaxis model with density-suppressed motility and general logistic source in an n-dimensional smooth bounded domain with Neumann boundary conditions. Under the minimal conditions for the density-suppressed motility function, we explore how strong the logistic damping can warrant the global boundedness of solutions and further establish the asymptotic behavior of solutions on top of the conditions.
Flexural strength and thermal properties of carbon black nanoparticle reinforced epoxy composites obtained from waste tires
Authors: Öner Gülşah Alar
Published: September 2022

In this study, both mechanical and thermal properties of epoxy@carbon black nanocomposite (EP@CB-NC) produced by adding 0.3, 0.6, and 1% by weight carbon black nanoparticle obtained from waste automobile tires to epoxy were investigated. The chemical and structural composition of EP@CB-NC was characterized by Fourier transform infrared spectroscopy (FTIR) in scanning electron microscopy (SEM). In addition, simultaneous thermo-gravimetric analysis (TGA), differential scanning calorimetry (DSC) experiments, and thermal conductivity measurements were performed to determine the thermal stability of the prepared EP@CB-NC. Increasing the strength of modified epoxy composites by means of nanoparticles obtained with waste may pave the way for obtaining new materials with clean environment and superior properties. The mechanical and thermal properties were improved by adding carbon black to the bare samples.
On synthesis of reversible circuits consisting of NOT, CNOT, 2-CNOT gates with small number of additional inputs
Authors: Zakablukov Dmitry V.
Published: September 2022

Reversible circuits consisting of NOT, CNOT and 2-CNOT gates with small number of additional inputs are considered. For such a circuit implementing a map

f
:

Z

2
n

→

Z

2
n

,

$f\colon \mathbb Z_2^n \to \mathbb Z_2^n,$

we study the Shannon complexity function L(n, q) under the condition that the number of additional inputs is q = O(n
2). For this range of q, it is shown that

L
(
n
,
q
)
≍
n

2
n

/

log
2

n
.

$L(n,q) \asymp n2^n \mathop / \log_2 n.$

We show that

L
(
n
,
q
)
≍
n

2
n

/

log
2

(
n
+
q
)

$L(n,q) \asymp n2^n \mathop / \log_2 (n+q)$

for all

q
≲
n

2

n
−
⌈
n

/

ϕ
(
n
)
⌉

,

$q \lesssim n2^{n-\lceil n \mathop / \phi(n)\rceil},$

where

ϕ
(
n
)
→
∞

and

n

/

ϕ
(
n
)
−

log
2

n
→
∞

as

n
→
∞
.

$\phi(n) \to \infty {\text {and}} \,n \mathop / \phi(n) - \log_2 n \to \infty \,{\text {as}}\, n \to \infty.$
Landuma: a case of radical alliterative agreement
Authors: Sumbatova Nina
Published: September 2022

The principal goal of this paper is to describe agreement in the Landuma language (Mel < Niger-Congo). Landuma shows agreement in animacy and, for inanimate nouns, radical alliterative agreement, a type of agreement conditioned by phonology: the first phoneme of the agreement prefix is conditioned by the first phoneme of the controlling noun. This type of agreement has much in common with agreement in noun class but is governed by essentially different mechanisms. Radical alliterative agreement is a challenge for linguistic theory, because it contradicts the generally adopted Principle of Phonology-Free Syntax and the understanding of phonological elements as unilateral entities. Radical alliterative agreement has been previously found in some other languages, first of all in the Kru languages (Niger-Congo) and in the Arapesh languages (New Guinea). The authors who have dealt with radical alliterative agreement have suggested a number of alternative descriptions in order to avoid theoretical problems. Some of the possible alternatives are also discussed in this paper.
Geoid model validation and topographic bias
Authors: Sjöberg Lars E.
Published: September 2022

Recently a number of geoid campaigns were performed to verify different types of geoid and quasigeoid modeling techniques. Typically, GNSS-leveling was employed as an independent method, but in some cases zenith camera astronomic deflection data were also used in astrogeodetic determinations of the geoid and/or quasigeoid. However, due to the uncertainty in the topographic density distribution data (and thereby in orthometric heights), we conclude that neither GNSS-leveling nor astrogeodetic techniques can reliably verify differences between gravimetric geoid models at several centimeter levels in rough mountainous regions. This is because much the same topographic data are used both in the gravimetric geoid models and in their verifications by geometric and/or astrogeodetic geoid models. On the contrary, this is not a problem in verifying gravimetric quasigeoid models, as they are independent of the topographic density distribution, and so is the related normal height used in GNSS-leveling.
{6,6′-((1E,1′E)-((2,2-dimethylpropane-1,3-diyl)bis(azaneylylidene))bis(methaneylylidene))bis(2-bromo-4-chlorophenolate)-κ4N,N′,O,O′}copper(II), C19H16Br2Cl2CuN2O2
Authors: Wu Qiong , Yu Guojun , Yang Jiao , Yang Qiuling
Published: September 2022

C19H16Br2Cl2CuN2O2, monoclinic, P21/n (no. 14), a = 12.4679(2)Å, b = 8.61760(10) Å, c = 19.7854(3) Å, β = 97.1610(10)°, V = 2109.23(5) Å3, Z = 4, R

gt
(F) = 0.0325, wR

ref
(F
2) = 0.0865, T = 170.0 K.
[2,2′-{Ethane-1,2-diylbis[(azanylylidene)methanylylidene]}bis(3-bromo-2-hydroxyphenyl)]iron(III) nitrate, C20H12Br2CuN2O2
Authors: Yang Jiao , Xu Jiajun , Liao Nichan , Wu Qiong
Published: September 2022

C20H12Br2CuN2O2, orthorhombic, Pbca (no. 61), a = 7.7117(2) Å, b = 18.7735(4) Å, c = 24.8518(4) Å, V = 3597.93(13) Å3, Z = 8, Rgt
(F) = 0399, wRref
(F
2) = 0.1125, T = 213 K.
The crystal structure of [(2,2′-bipyridine-κ2
N,N)-bis(6-phenylpyridine-2-carboxylato- κ2
N,O)nickel(II)] monohydrate, C34H26N4O5Ni
Authors: Tingting Zhou , Zhen Cao
Published: September 2022

C34H26N4O5Ni, monoclinic, C2/c (no. 15), a = 29.3459(13) Å, b = 10.2867(4) Å, c = 19.8780(9) Å, β = 107.243(5)°, V = 5730.9(4) Å3, Z = 8, Rgt
(F) = 0.0326, wRref
(F
2) = 0.0817, T = 200K.
Rechtliche Gestaltungsmöglichkeiten der Purpose Economy im Gesundheitswesen
Authors: Hoffmann Felix , Schumacher Helge
Published: September 2022

Seit einigen Jahren werden die Stimmen lauter, die die zunehmende Kommerzialisierung des Gesundheitswesens kritisieren. Die sogenannte Purpose Economy ist ein vielversprechender Ansatz für eine alternative privatwirtschaftliche Herangehensweise an die Art und Weise des Wirtschaftens. Anhand dieses Artikels wird zunächst eine Definition des Begriffes „Purpose Economy“ hergeleitet, um dann rechtliche Gestaltungsmöglichkeiten der Purpose Economy im Gesundheitswesen darzustellen.
Patientenpräferenzen in AMNOG-Nutzenbewertungen: Anspruch und Wirklichkeit
Authors: Hipp Wolfgang
Published: September 2022

Die Bewertung patientenrelevanter therapeutischer Effekte bildet den Kern der AMNOG-Nutzenbewertungen. Die Antworten des Gemeinsamen Bundesausschusses auf die entscheidende Frage der Patientenrelevanz klinischer Endpunkte sind bisher wenig evidenzbasiert. Es gibt vielversprechende Initiativen, das ungenutzte Potenzial der Erhebung von Patientenpräferenzen zur systematischen Bestimmung der Patientenrelevanz auszuschöpfen.
Proteomics characterization of the adenovirus VA1 non-coding RNA on the landscape of cellular proteome
Authors: Uddin Zafar , Khan Munazza , Höti Naseruddin , Khan Jabbar , Attar Roba
Published: September 2022

Objectives

Gene therapy using adenoviruses has shown tremendous promise in animal and human models in the past. The tumor responses achieved by these viruses were either because of their oncolytic properties or therapeutic genes expressed from the genome of the virus. We have previously shown enhanced viral replication in cell models that have lost or lower expression of the cyclin dependent kinases inhibitor (p21/Waf-1). Beside the early onset of many viral genes in the p21/Waf-1 knock out cell model, we observed a significantly higher copy number for the viral VA1 but non VA2 non-coding RNA transcripts. In this study we investigated the effect of adenoviral VA1 non-coding RNA on the landscape of cellular proteins.

Methods

Using a DU145 cell line as a model that was transiently transfected with a plasmid carrying the adenoviral VA1 non-coding RNA, we were able to study changes in the proteome and the cellular cascade of the cells. Using state of the art global proteomics analyses of the differentially expressed proteins between the VA1 overexpressed and control cells demonstrated how the early onset of VA1 transcript affected cellular machinery.

Results

Using a 1.5 fold cut off between the down-regulated or overexpressing proteins, we were able to demonstrate how the non-coding VA1 gene might be responsible for suppressing the proteasome degradation, the endocytic and lysosomal pathways. Similarly, overexpression of the non-coding VA1 transcript was responsible for the upregulation (1.5 fold) of approximately 40 different proteins in the spliceosome machinery.

Conclusions

These data demonstrated how the non-coding VA1 RNA functioned independently of any other viral proteins in modulating the cell signaling cascades to promote viral propagation. To our knowledge this is the first report to investigate the function of VA1 non-coding RNA on the cellular proteome.
The role of the European Union in the Brazilian foreign policy
Authors: Luiz Reis da Silva André , Volpato Vitória
Published: September 2022

This article aims to discuss the role of the European Union (EU) in Brazilian foreign policy by analyzing the Brazil-European Union Strategic Partnership. To do so, this investigation involves the use of qualitative data, literature review, and documentary research. Both Brazil and the EU do not present precise definitions for what they understand as strategic partnerships and, hence, this phenomenon must be framed through structural elements in these actors’ foreign policy. The European bloc is an important actor in Brazilian foreign policy, given the historic evolution of Brazil-EU relations, the establishment of the Strategic Partnership, and the EU’s economic relevance for the country. In turn, the current locus of the EU in Brazilian foreign policy is still unclear, due to overlapping levels of interaction between Brazil and the EU and to the difficulties in the domestic scenarios of both actors.
Sistemas eleitorais e integridade eleitoral
Authors: Rebouças Batista Ian , Lins Rodrigo
Published: September 2022

O que são sistemas eleitorais, como variam ao redor do mundo e quais as suas consequências para outros elementos da vida política de um país? Este artigo busca refletir sobre essas questões de maneira didática, percorrendo as principais famílias de sistemas eleitorais – majoritário, proporcional e misto – e variações dentro destas. Demonstramos ainda que sistemas eleitorais específicos não geram necessariamente eleições melhores ou piores. Com dados do International Institute for Democracy and Electoral Assistance e do Electoral Integrity Project mostramos que ainda que eleições com sistemas proporcionais apresentem consistentemente melhores índices de integridade eleitoral, isso não se dá em razão do sistema eleitoral em si, mas um elemento anterior influencia tanto a adoção de determinado sistema quanto na qualidade da eleição: a qualidade do regime. Autocracias, que por definição realizam eleições enviesadas, onde o incumbente não pode perder, adotam sistemas majoritários com maior frequência, enquanto democracias, que realizam eleições competitivas, adotam mais frequentemente o sistema proporcional.
Host Immune Responses to Arthritogenic Alphavirus Infection, with Emphasis on Type I IFN Responses
Authors: Hean Tang Patrick Chun , Hann Ng Wern , Liu Xiang
Published: September 2022

Arthritogenic alphaviruses, such as Ross River virus, chikungunya virus and O’nyong-nyong virus, cause endemic disease globally and are a major public health concern. The hallmarks of arthritogenic alphavirus disease are debilitating pain, and potentially chronic inflammation of the muscles, thus influencing quality of life. The type I IFN response is a major component of the innate immune response against arthritogenic alphaviruses, and is essential in inhibiting viral replication and dissemination. Type I IFNs are induced during early stages of infection and are essential for the activation of the antiviral innate immune response. They also link the innate immune response and the activation of adaptive immunity. This review focuses on the host immune response, particularly that involving type I IFN, in arthritogenic alphavirus disease.
A Gap-Junction Mutation Reveals That Outer Hair Cell Extracellular Receptor Potentials Drive High-Frequency Cochlear Amplification
Authors: Levic Snezana , Lukashkina Victoria A. , Simões Patricio , Lukashkin Andrei N. , Russell Ian J.
Published: September 2022

Cochlear amplification enables the enormous dynamic range of hearing through amplifying cochlear responses to low- to moderate-level sounds and compressing them to loud sounds. Amplification is attributed to voltage-dependent electromotility of mechanosensory outer hair cells (OHCs) driven by changing voltages developed across their cell membranes. At low frequencies, these voltage changes are dominated by intracellular receptor potentials (RPs). However, OHC membranes have electrical low-pass filter properties that attenuate high-frequency RPs, which should potentially attenuate amplification of high-frequency cochlear responses and impede high-frequency hearing. We madein vivointracellular and extracellular electrophysiological measurements from the organ of Corti of male and female mice of the CBA/J strain, with excellent high-frequency hearing, and from the CD-1 mouse strain, which has sensitive hearing below 12 kHz but loses high-frequency hearing within a few weeks postpartum. The CD-1 mouse strain was transfected with an A88V mutation of the connexin 30 gap-junction protein. By blocking the action of the GJ protein to reduce input resistance, the mutation increased the OHC extracellular RP (ERP) magnitude and rescued high-frequency hearing. However, by increasing the organ of Corti resistance, the mutation rescued high-frequency hearing through preserving the OHC extracellular RP (ERP) magnitude. We measured the voltage developed across the basolateral membranes of OHCs, which controls their electromotility, for low- to high-frequency sounds in male and female mice of the CD-1 strain that expressed the A88V mutation. We demonstrate that ERPs, not RPs, drive OHC motility and cochlear amplification at high frequencies because at high frequencies, ERPs are not frequency attenuated, exceed RPs in magnitude, and are appropriately timed to provide cochlear amplification.

SIGNIFICANCE STATEMENTCochlear amplification, which enables the enormous dynamic range of hearing, is attributed to voltage-dependent electromotility of the mechanosensory outer hair cells (OHCs) driven by sound-induced voltage changes across their membranes. OHC intracellular receptor potentials are electrically low-pass filtered, which should hinder high-frequency hearing. We measured the intracellular and extracellular voltages that control OHC electromotilityin vivoin a mouse strain with impaired high-frequency hearing. A gap-junction mutation of the strain rescued high-frequency hearing, increased organ of Corti resistance, and preserved large OHC extracellular receptor potentials but reduced OHC intracellular receptor potentials and impaired low-frequency hearing. We concluded intracellular potentials drive OHC motility at low frequencies and extracellular receptor potentials drive OHC motility and cochlear amplification at high frequencies.
Effect of intumescent coating on the charring rate of nail-laminated timber
Authors: Pang Sung-Jun , Ahn Kyung-Sun , Kim Min-Jeong , Hwang Sung-Wook , Kang Seog Goo , Kwak Hyo Won , Yeo Hwanmyeong , Oh Jung-Kwon
Published: September 2022

Intumescent coating was studied relative to the fire performance of nail-laminated timber. Three NLT specimens were coated with three different intumescent coating thicknesses (1, 2, and 3 mm) in even-numbered laminae and compared to uncoated NLT specimens. As a result of the coating, the internal temperature of the coated specimen increased more slowly than that of the uncoated specimen. The average charring rate of the intumescent coating specimen was reduced by 12.8% (1-mm thickness), 14.1% (2-mm thickness), and 15.4% (3-mm thickness) compared with the uncoated specimen. However, statistical analysis showed there was no significance between 1-, 2-, and 3-mm coating thicknesses. The combustion of wide surfaces of timber laminae between the plywood was delayed due to the coated plywood, and the timber laminae became a one-dimensional charring rate problem. Therefore, if even laminae are coated with an intumescent, then the NLT can be designed with a one-dimensional charring rate condition.
Examining the Association Between MIS-C and the Child Opportunity Index at a Single Center
Authors: Tyris Jordan , Boggs Kaitlyn , Bost James , Dixon Gabrina , Gayle Tamara , Harahsheh Ashraf S. , Sharron Matthew P. , Majumdar Suvankar , Krishnan Anita , Smith Karen , Goyal Monika K. , Parikh Kavita
Published: September 2022

OBJECTIVE

To describe associations between the Child Opportunity Index (COI) and multisystem inflammatory syndrome of childhood (MIS-C) diagnosis among hospitalized children.

METHODS

We used a retrospective case control study design to examine children ≤21 years hospitalized at a single, tertiary care children’s hospital between March 2020 and June 2021. Our study population included children diagnosed with MIS-C (n = 111) and a control group of children hospitalized for MIS-C evaluation who had an alternative diagnosis (n = 61). Census tract COI was the exposure variable, determined using the patient’s home address mapped to the census tract. Our outcome measure was MIS-C diagnosis. Odds ratios measured associations between COI and MIS-C diagnosis.

RESULTS

Our study population included 111 children diagnosed with MIS-C and 61 children evaluated but ruled out for MIS-C. The distribution of census tract overall COI differed significantly between children diagnosed with MIS-C compared with children with an alternate diagnosis (P = .03). Children residing in census tracts with very low to low overall COI (2.82, 95% confidence interval [CI]: 1.29–6.17) and very low to low health/environment COI (4.69, 95% CI 2.21–9.97) had significantly higher odds of being diagnosed with MIS-C compared with children living in moderate and high to very high COI census tracts, respectively.

CONCLUSION

Census tract child opportunity is associated with MIS-C diagnosis among hospitalized children suggesting an important contribution of place-based determinants in the development of MIS-C.
Bioproductos como sustitutos parciales de la nutrición mineral del cultivo de pimiento (Capsicum annuum L).
Authors: Terry Alfonso Elein , Ruiz Padrón Josefa , Rivera Espinosa Ramón , Falcón Rodríguez Alejandro , Carrillo Sosa Yudines
Published: September 2022

Los Bioproductos, ejercen efectos beneficiosos en las plantas como la estimulación del crecimiento y el rendimiento agrícola, así como la inducción de mecanismos defensivos, además de no ser dañinos a las plantas ni al medio ambiente. El presente trabajo se desarrolló entre los meses enero a abril de 2019 bajo condiciones de cultivo protegido, con el objetivo de evaluar la efectividad agrobiológica de diferentes Bioproductos en el cultivo del pimiento (Capsicum annuum L) como sustitutos parciales de la nutrición mineral, así como en el estímulo del crecimiento y rendimiento del cultivo. Se desarrolló en un suelo Ferralítico Rojo, bajo un diseño completamente aleatorizado con cinco tratamientos y cuatro repeticiones. Los resultados mostraron un efecto positivo de la combinación micorrizas con bioestimulantes para lograr un mayor crecimiento de las plantas en la fase de semillero, con un acortamiento de este ciclo de 10 días con respecto al tratamiento control. En la fase de trasplante, el mayor desarrollo y rendimiento del cultivo se logró con la combinación micorrizas – Quitosana o Bionutriente, combinada con el 75 % de la fertilización mineral (NPK), lo que permitió disminuir el 25 % de la misma. En el caso de las restantes combinaciones de bioproductos – fertilizante mineral, se demuestra que estos no son suficientes para lograr mantener el rendimiento del cultivo bajo este sistema.
Morphological correlation and quality of Agonandra brasiliensis seedlings in substrates and controlled release fertilizer
Authors: Smiderle Oscar José , Souza Aline das Graças , Montenegro Raiovane Araújo , Moriyama Thiago Komuro
Published: September 2022

The objective was to investigate the effect of substrate compositions with and without the addition of controlled release fertilizer (CRF) and to determine the correlations between the morphological characteristics of Agonandra brasiliensis. The variables studied were: shoot height, collet diameter, increase of height of shoot and collet diameter, shoot dry weight, root dry weight, total dry weight, the relation between the dry matter weight of the shoot and the dry matter weight of the roots, the relation between shoot height and collet diameter, the relation between shoot and root dry weight, and Dickson quality index. The results of the total dry weight of the ivory-wood plants allowed to infer a positive influence of the addition of CRF in the Sub 1 (8.3 g plant-1), Sub 2 (8.2 g plant-1), and Sub 4 (7.3 g plant-1) showing an increase of 72.2  %, 52.4 %, and 68.5 % respectively, when compared to Sub 1 (2.3 g plant-1), Sub 2 (3.9 g plant-1), and Sub 3 (2.3 g plant-1) without CRF addition. The substrates (sub 5) and (sub 1) with addition of CRF are indicated for the production of seedlings of Agonandra brasiliensis.
Evaluación de subproductos de pepino cohombro (Cucumis sativus) y granadilla (Passiflora ligularis) como fuente de obtención de pectinas.
Authors: Ramirez Alvarado Rafael Andres , Aponte Cárdenas Andrea , Benavides Barrantes Yamile , Ortiz Rojas Yamid
Published: September 2022

En el presente estudio, se empleó el exocarpio pepino cohombro (Cucumis sativus) y mesocarpio de la granadilla (Passiflora ligularis), como fuentes promisorias de pectina para la industria. Se empleó hidrolisis acida y se analizaron dos niveles de pH y dos tiempos de hidrolisis. La calidad de las pectinas obtenidas fue evaluada (Humedad, pH final, índice de metoxilo, porcentaje de esterificación, ácido galacturónico, análisis IR) y los resultados fueron comparados con un estándar comercial y literatura.Los análisis realizados mostraron que las pectinas obtenidas a partir de estas dos fuentes exhiben excelentes características en comparación a los estándares pedidos para este tipo de material. También se identificaron las condiciones que maximizan el rendimiento de extracción, generando una alternativa para el manejo de subproductos de industrias relacionadas.
Capacidad emulsificante y estabilidad de emulsiones de harinas de cascarilla de arroz (Oryza sativa) bajo diferentes condiciones de pH y fueza iónica
Authors: Arroyo Arboleda Michell Alejandra , Rendón Benjumea Alejandra Estefanía , Martínez Giron María Isabel , Serna Cock Liliana
Published: September 2022

Aunque el contenido de proteína en la cascarilla de arroz es mínimo, estas proteínas en conjunto con su contenido de fibra representado en celulosa, hemicelulosa y lignina podrían tener interesantes propiedades tecno-funcionales para la agroindustria alimentaria y no alimentaria.  Se obtiene harina  de cascarilla de arroz (Oryza sativa L.) y se evalua su capacidad emulsificante (CE) y la estabilidad de la emulsión (EE). La CE se evalua a dos tamaños de partícula (>250 y <250 μm), tres pH (5.5; 2.5 y 1.5) y tres fuerzas iónicas (0, 0.2 y 0.5 M de NaCl). En la EE se evalua a las mismas condiciones y además se evalua el efecto de la temperatura (50 y 80 °C). Se encuentra que la harina de cascarilla de arroz a tamaño de partícula >250, pH de 5.5, y fuerza iónica de 0.5 M de NaCl presenta la mejor CE (3.3 ± 0.03 mL/g). La menor CE (1.5 ± 0.0 mL/g) se obtiene a un tamaño de partícula < 250 μm, pH 1.5 y fuerza iónica de 0.5 M de NaCl. La mayor EE (47.2 ± 0.03 % ) se obtiene a 50 °C, tamaño de partícula > 250 μm, pH 5.5, y fuerza iónica de 0.2 M de NaCl. Se comprueba que la harina de cascarilla de arroz tiene propiedades emulsificantes y estas propiedades se ven influenciadas por factores extrínsecos como el tamaño de partícula, el pH, la fuerza iónica, y la temperatura. Lo anterior indica que la harina de cascarilla de arroz es una alternativa al uso de emulsificantes de origen animal, por lo cual podría utilizarse muy particularmente en preparaciones veganas, donde su contenido en sílice debe revisarse, pero además podría utilizarse en la elaboración de estructuras donde las propiedades emulsificantes son importantes, como lo son las industrias cosmética y de bloques de concreto, por ejemplo.
Aptitud combinatoria del rendimiento en líneas endogámicas de uchuva (Physalis peruviana L.)
Authors: Rosero Erazo Jhon Alexander , Garcia Davila Mario Augusto , Lagos  Burbano Tulio Cesar , Duarte Alvarado David Esteban , Lagos Santander Liz Katherine
Published: September 2022

La uchuva Physalis peruviana originaria de los valles interandinos de América del Sur, es actualmente uno de los frutos exóticos de mayor relevancia para Colombia; su demanda para exportación busca que se posea características ideales de rendimiento y calidad de fruto. El objetivo es determinar los parámetros genéticos de Aptitud Combinatoria de nueve líneas endogámicas de uchuva bajo condiciones de la región natural Andina del departamento de Nariño. Se evalúan 36 híbridos de uchuva obtenidos a partir de nueve líneas endogámicas 12U347, 12U350, 12U352, 12U357, 12U360, 12U368, 12U374, 12U377, y 12U39, en cuatro localidades del departamento de Nariño: Gualmatán, Puerres, Pasto e Ipiales, con el fin de determinar los efectos de aptitud combinatoria general (ACG) y específica (ACE) sobre el rendimiento. Se evalúa el rendimiento comercial (RTOC) y el rendimiento agronómico (RTOA), en un dialélico bajo el método IV modelo fijo de Griffing (1956). El análisis de varianza combinado indica diferencias significativas en la interacción genotipo ambiente para RTOC y RTOA a través de todas las localidades. El análisis de Aptitud Combinatoria reporta que en las RTOA y RTOC son importantes los efectos aditivos y no aditivos. Los mejores híbridos en Gualmatán son 7x8, 3x5 y 3x7; en Ipiales 2x9, 1x3 y 4x8; en Pasto 1x7, 1x3 y 7x8, y en Puerres 2x5, 2x8 y 2x3, los cuales pueden ser tenidos en cuenta para ser usados como híbridos comerciales de alto rendimiento.
Yield, quality, and nutrient uptake of stevia under continental Mediterranean climate
Authors: Matías Prieto Javier , Cruz Sobrado Verónica
Published: September 2022

Stevia (Stevia rebaudiana Bertoni) is gaining attention due to its sweetening power. The stevia crop is still relatively unknown in Europe, and very little is known about its nutrient requirements. Therefore, agronomic studies are needed.  Field trials were carried out in inland Spain in 2014 and 2015, in order to evaluate the yield, quality, and nutrient requirements of stevia according to planting density and harvest regime, under a continental Mediterranean climate. In 2014, the dynamics of growth, quality and accumulation of nutrients were studied during the vegetative period according to three planting densities (5.0, 7.5, and 10.0 plants m-2). No significant influence was found. The steviol glycosides concentration decreased sharply at flowering, while the leaf and steviol glycosides yields continued to increase for another 30 days or more. Yield, quality and nutrient uptake were studied during 2015 according to the three same planting densities, and three harvest regimes (one, two, and three cuts per year; all before flowering). Both factors had significant influence on most of the studied parameters. The best quality and the highest yield were not obtained under the same crop management. The highest yield was achieved with 10 plants m-2 and with 2 cuts per year, achieving around 6000 kg ha-1 of dry leaf and 650 kg ha-1 of steviol glycosides. The best quality was achieved with one cut just before flowering (12.2 % of steviol glycosides, 0.35 of Reb A to Stev ratio). The average uptakes of N, P, K, Ca, Mg and S were 35.6, 4.8, 59.9, 14.2, 5.2, and 1.6 kg t-1 of leaf.