bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation, selection and characterization of salt and temperature tolerant plant growth promoting rhizobacteria.
Authors: SATYAM SATYAM
Published: August 2023

The present study was focused on isolation, selection and characterization salt and temperature tolerant plant growth promoting rhizobacteria from rhizosphere soil of tomato. Out of one hundred thirty eight samples total forty eight isolates grown on Ashby’s Mannitol agar media. For assessment of salt and temperature tolerancy the medium was supplemented with different NaCl concentrations, MgCl2 concentration and different pH level. All the forty eight isolates showed promising salt tolerance. Out of forty eight isolates nine isolates were extremely (6% NaCl) salt tolerant (more than 5.4% salt tolerance limit). Two isolate were tolerant to NaCl concentration (2.10–3.6% salt). Nine isolates were moderately tolerant to NaCl concentration (0.09–1.50% salt). Twenty six isolates were moderately sensitive to NaCl concentration (0.15–0.60% salt). Classification of salt and temperature tolerant plant growth promoting rhizobacteria was done on the basis of salt tolerance limit. Out of forty eight isolates, eight isolates showed growth at MgCl2 concentration of 4.0%, two isolates showed growth at 3.0% MgCl2 concentration, thirteen isolates showed growth at 2.0% MgCl2, fifteen isolates showed growth at 1.0% MgCl2 and eight isolates showed growth on 0.5% MgCl2 concentration. Among forty eight isolates, eight isolates showed growth at 9.0 and remaining thirty eight isolates showed growth at pH 7.0. Out of forty eight isolates, four isolates (STTA8- Pseudomonas furukawaii, STTA12- Achromobacter sp, STTA24- Agrobacterium pusense and STTA39- Agrobacterium pusense) were showed plant growth promoting activity test positive. STTA8- Pseudomonas furukawaii, STTA12- Achromobacter sp. Shown temperature tolerance limit 600c and STTA24- Agrobacterium pusense and STTA39- Agrobacterium pusense shown temperature tolerance limit 500c.The work was carried out for the isolation, selection and characterization of salt and temperature tolerant plant growth promoting rhizobacteria which would be highly important inoculum to improve the growth and development of the tomato plant under saline environment. There is currently need to develope highly salt and temperature tolerant plant growth promoting rhizobacterial consortium.
Autonomous Navigation of Wheelchairs in Indoor Environments using Deep Reinforcement Learning and Computer Vision
Authors: Afonso Paulo de Almeida , Ferreira Paulo R.
Published: August 2023

We introduce an innovative approach to autonomous navigation for motorized wheelchairs in shared indoor spaces with human presence, combining Deep Reinforcement Learning and Computer Vision. The central aim of this study is to enhance the well-being of individuals with disabilities who rely on such assistance for their mobility needs. Our methodology merges the Deep Deterministic Policy Gradient (DDPG) algorithm with advanced computer vision techniques, empowering motorized wheelchairs to navigate through environments that encompass both stationary and moving people. Comparative tests were conducted between the DDPG and Deep Q-Network (DQN) algorithms, spanning four distinct stages. Each stage represented two different scenarios from the training environment, characterized by complexity levels exceeding those to which the robot had been trained.
The DDPG algorithm showcased its superior efficiency and stability compared to DQN. Across all analyzed stages, DDPG exhibited higher average success rates: 98\% (Stage 01), 89\% (Stage 02), 86\% (Stage 03), and 86\% (Stage 04), demonstrating exceptional generalization capabilities and consistently outperforming the training environment in diverse settings. In contrast, DQN struggled to avoid collisions, resulting in significantly lower average success rates: 3\% (Stage 02), 14\% (Stage 03), and 29\% (Stage 04). Our findings underscore the promising potential of our proposed solution, thereby contributing to the progress of research in this particular domain.
Constructing Origin-Destination Matrix using Wi-Fi and AFC Data
Authors: Silva Diego Da , Elsaid Feras , Shalaby Amer
Published: August 2023

Transportation systems planning and management rely heavily on Origin-Destination (OD) demand matrices. However, the traditional approach of creating these matrices using household travel survey data is not only time-consuming but also expensive, making it challenging to apply them to detailed and time-sensitive analyses. Automated fare collection (AFC) systems can provide a solution to these challenges. However, many transit fare systems are entry-only with no requirement to tap at the exit station, which makes it challenging to determine the location of alighting stations and analyze spatial demand patterns. This study proposes a framework that uses Wi-Fi traces and passenger counts at AFC entry/exit gates to construct OD matrices for entry-only Urban Rail Transit (URT) systems. The City of Toronto's subway system was used as a case study, and the framework was compared to 2016 Transportation Tomorrow Survey (TTS), which is the primary source of OD-matrix estimation in the city. The generated OD-matrices were found to be very close to the OD-matrix from the household travel survey, with a cosine similarity close to one for most subway regions. Our estimated OD-matrices offer several advantages over traditional methods. They have low matrix sparsity and fast computational time and convergence, and they exhibit strong capability of recognizing demand patterns at the station-level.
The effect and mechanisms of fructose exposure on amino acid metabolism among Chinese community residents
Authors: Rang Ouyan , Qin Xinru , Tang Yonghong , Li Guojuan , Liu Xiaocheng , Wang Mu , Zhong Jing
Published: August 2023

The consumption of fructose has increased dramaticly during the last few decades, inducing a great increase in the risk of intrahepatic lipid accumulation, hypertriglyceridemia, hyperuricemia and cancer. However, the underlying mechanism has not yet been fully elucidated. Amino acid metabolism may play an important role in the process of the diseases caused by fructose, but there is still a lack of corresponding evidence. In present study, we provide an evidence of how fructose affects amino acids metabolism in 1895 ordinary residents in Chinese community using UPLC-QqQMS based amino acid targeted metabolomics and the underlying mechanism of fructose exposure how interferes with amino acid metabolism related genes and acetylated modification of proteome in the liver of rats model. We found people with high fructose exposure had higher levels of Asa, EtN, Asp, and Glu, and lower levels of 1MHis, PEtN, Arg, Gln, GABA, Aad, Hyl and Cys. The further mechanism study displayed amino acid metabolic genes ofAspa,Cndp1,Dbt,Dmgdh,and toxic metabolites such as N-acetylethanolamines accumulation, interference of urea cycle, as well as acetylated modification of key enzymes in glutamine metabolic network and glutamine derived NEAAs synthesis pathway in liver may play important roles in fructose caused reprogramming in amino acid metabolism. This research provides novel insights of the mechanism of amino acid metabolic disorder caused by fructose and supplies new targets for clinical therapy.
Exploring of the shared gene signatures and molecular mechanism in COVID-19 and tuberculosis
Authors: Guo Jiwei , Li Jing , Liu Mingyue , Gao Chengpeng , Fang Jianwei , Wang Zhiliang , Xu Hao , Bi Hengtai , Wang Yousen , Jiang Junjie
Published: August 2023

Background
After the Coronavirus Disease 2019 (COVID-19) pandemic, tuberculosis (TB) incidence has demonstrated a noticeable upswing, with the causative linkage and mechanistic crosstalk between these conditions remaining uncharted. This study endeavours to decipher the communal genetic elements and underlying molecular interplayunderlying COVID-19 and TB.
Methods
The Gene Expression Omnibus (GEO) served as the repository for sourcing RNA sequence datasets pertinent to COVID-19 and TB. Leveraging the R software, the Weighted Gene Co-expression Network Analysis (WGCNA) and limma package facilitated the uncovering of a co-expression network intertwined with both COVID-19 and TB. Shared genes underwent enrichment analysis via ClueGO, while hub genes within the COVID-19 and TB context were identified through MCODE based on Cytoscape software. An array of machine learning algorithms – Random Forests (RF), Least Absolute Shrinkage and Selection Operator (LASSO) Logistic Regression, and Support Vector Machine-Recursive Feature Elimination (SVM-RFE) – guided the further isolation of key genes. We also constructed the nomograms, and assessed the predictive prowess by evaluating the Area under the curve (AUC), calibration curves, decision curve analysis (DCA) and clinical impact curves. The immune microenvironment (TIME) in TB was analyzed using CIBERSORT, allowing for the assessment of correlation between key genes and immune cells.
Results
WGCNA analyses and gene expression differences analysis based on the Limma divulged a set of 281 shared differential genes between TB and COVID-19. Enrichment analysis elucidated their association with a variety of biological functions and signaling pathways, such as response to interferon-γ, NOD-like receptor signaling pathway, and influenza A. Machine learning facilitated the identification of GBP5 and IFITM3 genes, which were subsequently fashioned into nomograms, exhibiting solid clinical relevance (AUC = 0.9854, Mean Absolute Error = 0.009). CIBERSORT analysis uncovered substantial shifts in multiple immune cells in TB, notably Macrophages.M1, Dendritic.cells.activated, and Neutrophils cells, which revealed strong correlation with the expression of GBP5 and IFITM3 in TB.
Conclusion
In this study, we obtained the shared gene between COVID-19 and TB and preliminarily revealed its function. In addition, GBP5 and IFITM3 could serve as key genes among the shared genes and were associated with a variety of immune cells.
Development of efficient genetic-transformation- and genome-editing systems, and the isolation of a CRISPR/Cas9-mediated high-oil mutant in the unicellular green alga Parachlorella kessleri strain NIES-2152
Authors: Kasai Yuki , Takagi Satsuki , Ota Shuhei , Ishii Kotaro , Takeshita Tsuyoshi , Kawano Shigeyuki , Harayama Shigeaki
Published: August 2023

Background
Parachlorella kessleri strain NIES-2152, a unicellular green alga, has the characteristics of rapid growth and high lipid productivity; thus this strain has been considered to be a candidate for use in biofuel production. However, the commercialization of biofuels requires significant cost reduction, and strain improvements may be needed to achieve this objective.
Results
In the first part of this study, we developed a genetic transformation method applicable to P. kessleri as follows. During the cell cycle of P. kessleri, daughter cells develop within the mother cell wall. Therefore, P. kessleri cells are encompassed by two cell-wall layers: a daughter cell wall and a mother cell wall, during most of its cell cycle except for a brief period after the cleavage of the mother cell wall. We found that cells just hatched out of the mother cell wall exhibited high efficiency in taking up DNA through electroporation. After optimizing the electroporation conditions, we achieved a transformation efficiency of 3.2 to 4.6 × 10−5 transformants per input cell.
Previously, we had isolated a mutant of P. kessleri named strain PK4 that accumulated higher concentrations of lipids than the wild-type strain. Resequencing of the PK4 genome had identified three genes with potential defects associated with the high lipid phenotype. In the second part of this study, we delivered Cas9-gRNA ribonucleoproteins (RNPs) targeting each of the three genes into P. kessleri cells using the same protocol as the genetic transformation, and successfully disrupted these three genes separately. The disruptants of one gene encoding a plastidic ATP/ADP translocase exhibited >30% higher lipid productivity than that of the wild-type strain under diurnal conditions.
Conclusions
We established an effective gene-editing method applicable to P. kessleri. Using this method, we succeeded in creating strains showing significantly higher lipid productivity than the wild-type strain, especially under diurnal rhythms.
Interactions of socioecological factors on the supply-demand balance of ecosystem services in the Yangtze River Economic Belt, China
Authors: Zhang Zhiming , Fang Fengman , Cheng Xiaojing
Published: August 2023

A comprehensive understanding of how socioecological factors can influence multiple ecosystem services (ES) may provide stakeholders with a management tool to coordinate economic development and environmental protection. However, previous studies have focused more on the supply side than on the demand of ESs. Furthermore, the underlying mechanisms for the changes in the supply-demand balance of ESs and their multiple drivers remain unclear. Therefore, in this study, an integrated theoretical framework was developed to assess the interactions of socioecological factors, including land use and cover, social economy, climate, and topography, on the variation in the ES supply, demand, and balance, which were mapped at the city scale in the Yangtze River Economic Belt (YREB). A variance inflation factor (VIF) was employed to detect the multicollinearity of the factors, and the sum of Akaike weights were used to simplify driving factors and identify essential driving factors. We employed variation partitioning analysis (VPA) to reveal the effects of unique and combined drivers on the ES supply, demand and balance. The results showed that undersupply cities (11.93% of the area) were concentrated within the three national urban agglomerations and increased in area from upstream to downstream, whereas oversupply cities (88.07%) were mainly distributed in the surroundings of the urban agglomerations. According to the VPA, the essential driving factors could effectively explain the variation in the ES supply, demand, and balance in the YREB. More importantly, these driving factors were simplified with no significant decrease in explanatory power. In the oversupply cities, the ES changes were determined by socioeconomic factors (urbanization rate and population density), land use/land cover (cropland, woodland, and unused land), and their interactions. In the undersupply cities, population density (PD) could explain the majority of the variation in ESs. However, the effects of climate and topography on ESs were more prominent at the scale of all cities in the YREB. Besides, PD displayed a significant negative correlation with ES supply and balance, whereas it was positively related to ES demand in the undersupply cities. These findings contribute to a comprehensive understanding of the interactions of socioecological factors on the supply-demand balance of ESs. This study is informative for human well-being and sustainable socio-economic development of the region.
Headaches in children after transcatheter device closure of atrial septal defects: a single centre experience
Authors: Griesman Joshua D , Marshall Audrey C
Published: August 2023

Background
Transcatheter device closure (TDC) is the most common treatment for isolated atrial septal defects (ASD) in children. In the adult population, the incidence of new-onset migraine headache after TDC is well-recognized, and is estimated at 15%. New-onset headache has not been well-described in an exclusively pediatric population. We reviewed our center’s experience to estimate the rate of headache complaints among pediatric patients undergoing TDC.
Methods
We performed a single-center retrospective review of all children undergoing TDC between January 1, 2018 and December 31, 2021. Among included patients, we comprehensively reviewed the electronic medical record (EMR), to identify patients reporting headache.
Results
165 consecutive patients underwent TDC during the study period. Of these, 134 met inclusion criteria. 20 (14.9%) patients had headache documented in the EMR. Of those, 7 (7/134, 5%) had persistent headaches (lasting greater than four weeks) or required further investigations. Two patients had headaches that were investigated with a brain MRI, which demonstrated normal or non-specific findings. One patient with a prior history of migraine required admission to hospital for migraine exacerbation. Another required emergency room management for status migrainosus. In addition to symptomatic pain management, 3 patients had a change in their anti-platelet regimen changed to clopidogrel. All patients with persistent headache were female (p < 0.1).
Conclusions
Our study demonstrated persistent headaches in 5% of children who underwent TDC. It is important for practitioners to discuss this association prior to TDC. Determination of the true incidence will require focused prospective data collection.
An evaluation of the replacement of animal-derived biomaterials in human primary tissue culture
Authors: Bramwell Laura , Gould Samantha , Davies Merlin , Trusler Emily , McMullan Conor , Harries Lorna
Published: August 2023

The ability of newly developed drugs to navigate the current translational pipeline is extremely poor, with less than 10% of drugs making this transition even after entry into clinical trials. There are many reasons for this, but interspecies differences in functional and physiological parameters contribute to the problem. Improving the humanrelevance of early pre-clinical in vitro models may help translatability, especially when targeting more nuanced species-specific cell processes. We aimed to define a set of guidelines for effective transition of human primary cells of multiple lineages into a more physiologically relevant, translatable, animal-free culture environment by systematic replacement of animal-derived biomaterials in in vitro culture systems, followed by assessment of effects on cell kinetics and phenotype. We successfully eliminated animalderived biomaterial from primary human dermal fibroblast, uterine fibroblast, pulmonary fibroblast, retinal endothelial cell, and peripheral blood mononuclear cell culture systems and defined the individual requirements of each cell subtype for transition to animal-component free culture conditions. We therefore demonstrate that it is possible to transition (“humanise”) a diverse set of human primary cell types by following a set of simple overarching principals that inform the selection, and guide the evaluation of new, improved, human-relevant culture conditions.
Complex Fermatean Fuzzy Partitioned Maclaurin Symmetric Mean operators and their Application to Hostel Site Selection
Authors: Azeem Muhammad , ali jawad , ali jawad
Published: August 2023

In relation to multi-criteria group decision making (MCGDM) problems where the evaluated value of each criteria is represented as complex Fermatean fuzzy sets, a novel approach to MCGDM is formulated. Firstly, a new aggregation operator, termed the partitioned Maclaurin symmetric mean (PMSM) operator, is introduced to address situations where criteria are partitioned into differ-ent parts, and there exist interrelationships among multiple criteria within the same part, while criteria in different parts are independent. The desirable properties of the PMSM operator are thoroughly investigated. To mitigate the adverse effects of unreasonable evaluation values of criteria on the aggregated result, complex Fermatean fuzzy power partitioned Maclaurin symmetric mean (CFFPPMSM) operators are further proposed. These operators combine the PMSM operator with the power average (PA) operator within complex Fermatean fuzzy sets. The weight of the crieria are taken unknown and and using the statistical method. In conclusion, a numerical instance is furnished to exemplify the application of the proposed approach in determining the optimal location for a university hostel, and a comparative analysis is conducted to demonstrate the advantages of the proposed methodology in handling complex Fermatean fuzzy MCGDM problems.
Cooperative H-arq Employing Powerful Totally Decomposed Cumulative Goppa Codes
Authors: Waweru Daniel Kariuki , Yang Fengfan , Zhao Chunli , Paul Lawrence Muthama
Published: August 2023

This paper presents a multi-relay Coded-Cooperative system that integrates Hybrid Automatic Repeat Request and Coded Cooperative Cooperation. It utilizes powerful cyclic Totally Decomposed Cumulative Goppa codes (TDCG codes) known for exceptional performance as best-known linear codes. The destination code is jointly designed by combining TDCG codes from relay and source codes. Optimized relay channel codes improve overall system bit-error-rate performance, aided by a proposed optimization technique. Numerical simulations assess BER performance under various channel conditions with BPSK modulation and coherence detection over flat Rayleigh fading channels. Results show that the TDCG coded-cooperative scheme outperforms non-cooperative approaches and similar coded cooperation techniques with comparable code rates. The integration of HARQ and Coded Cooperative Cooperation enhances wireless communication system reliability and efficiency.
Kaposi’s sarcoma-associated herpes virus (KSHV) do not imply the vast majority of Sri Lankan oral cancer patients
Authors: Perera Manosha Lakmali , Perera Irosha , Gunathilake Sharika
Published: August 2023

Human Herpes virus 8 (HHV 8) better known as Kaposi’s sarcoma-associated herpes virus (KSHV) frequently relates to malignancies in acquired immune deficiency syndrome (AIDS) patients. Interestingly, one incident reported in a head and neck cancer patient who is also a HIV patient. In the light of these findings, this original research aimed to detect the HHV-8 status in oral squamous cell carcinoma tissues of Sri Lankan male patients. Incisional biopsies of cases were collected, transported, stored, and dispatched as frozen tissues at -800 C. A sub case sample selected from a large case control study to represent the vast majority of the oral cancer patients in Sri Lankan context as oral cancer remains number 1 among Sri Lankan males. The DNA was extracted from frozen specimens using Gentra Puregene Tissue kit (Qiagen, Germany), solid tissue protocol according to the manufacturer’s instructions. The real-time PCR was performed with the specific primers to find out the possible implication of this virus on the vast majority of Sri Lankan oral cancer patients with established socio-demographic and risk factor profiles. Data belonged to Sociodemographic, oral hygiene practices, oral hygiene status, clinical and oral risk habit categories were obtained by a pre tested interviewer administered questioner were entered and analyzed using SPSS-21 Statistical Package. However, the HHV-8 DNA did not find in 22 OSCC cases. Kaposi’s sarcoma-associated herpes virus (KSHV) does not imply the vast majority of Sri Lankan oral cancer patients. Powered studies on minority [the high risk human papilloma virus (HPV) associated] oral cancer patients are recommended as transmission of both HPV and KSHV seems same among homosexuals.
Statistical Study of Ectopic Pregnancy in Al-Qutaifah National Hospital
Authors: Mushaweh Aghiad Anas , Alfawares Maamoun , Al-Sakka Kanaan Salah Al-Din
Published: August 2023

Background Ectopic pregnancy is one of the most dangerous conditions that significantly impact a woman's health and fertility, necessitating swift diagnosis and early effective intervention, and aim of this study is to determine the incidence rate of ectopic pregnancy among patients at Al-Qutaifah National Hospital, as well as to investigate the localization, symptoms, risk factors, and treatment methods associated with ectopic pregnancy.
Methods and materials: A retrospective study design was employed, involving the collection of patient-related information, detailed clinical histories, procedures undertaken, and outcomes achieved, all documented in a specialized form, the study was conducted in the Obstetrics and Gynecology Department at Al-Qutaifah National Hospital, the study spanned a period of 9 months, from December 1, 2022, to August 1, 2023.
Results: The study revealed an ectopic pregnancy incidence rate of 2.5%. The most common location for ectopic pregnancy was the fallopian tube, accounting for 80% of cases. A common risk factor for ectopic pregnancy occurrence was a history of prior surgical procedures or cesarean section. As for symptoms, all patients exhibited amenorrhea accompanied by vaginal bleeding and discharge of bloody material
Conclusion: The study was conducted on ectopic pregnancy, its placement and risk factors, as the study included 800 patients, 20 of whom suffered from ectopic pregnancy, with a rate of 2.5%, and the placement in the fallopian tubes was the most frequently seen, One of the most important risk factors associated with an ectopic pregnancy is having a previous cesarean section or pelvic surgery, Patients with ectopic pregnancy suffer from amenorrhea and bloody vaginal discharge with changes in the value of the human reproductive development.
Integration and Validation of a Numerical Pcm Model for Building Energy Programs.
Authors: BENACHIR NOUHAILA , Farida Bendriaa , MOHIB TAOUFIQ
Published: August 2023

The envelope of buildings has remained for a long time a passive means of limiting heat loss or heat gain on the one hand, to ensure a certain air conditioning by its thermal inertia. The increase in peak electricity demand in recent years has stressed the importance of peak electricity demand shifting technologies. The overall objective of the project is to improve the living conditions of the local population. The specific objective is to set up a pilot unit using local building materials, with low environmental impact, provided with organic phase change materials also the main objective here is to model, quantify and optimize the impact of the presence of PCMs in a thermal zone subjected to the climatic conditions of Morocco with the ultimate goal of developing low energy buildings and crated the comfort zone . First, a complete numerical model of the thermal behavior of a zone will be developed using the Trnsys, with the possibility of including phase change materials. The achievement of this objective will allow to have available a customized code, fully scalable, able to calculate all the thermal variables of interest in a building interacting with the environment. Such a complex wall technology integrating PCMs must be properly taken into account in order to simulate the energetic behavior of buildings and to evaluate their impact in several domains (environmental, thermal behavior of buildings). Once the thermal characterization of the building has been performed.
Phase Change Materials (PCMs) have a potential to improve the building envelope by increasing the thermal mass as well as contribute to a signify cant peak shift in whole building power demand. Therefore, special attention is given to properly capture the thermal behavior of PCMs in advanced building energy modeling software. Design of effective PCM thermal storage systems requires accurate energy modeling. There are analytical and numerical models developed during last few decades for this purpose, many have not been fully validated. Based on the current status of literature, the study identifies the limitations and drawbacks of existing methods. A parametric study is conducted to identify the optimum PCM thermo-physical properties,
Entwicklung eines ELISAs zur spezifischen Detektion von S100A12 Hexamer
Authors:   , Weber Jonas
Published: August 2023

S100A12 ist ein Entzündungsmarker, der inflammatorische Prozesse präzise anzeigt. Entzündungsprozesse mit erhöhten S100A12 Konzentrationen spielen vor allem bei Autoimmunerkrankungen wie der der rheumatischen Arthritis (RA), autoinflammatorischen Erkrankungen wie der juvenilen idiopathische Arthritis (JIA) oder weiteren Erkrankungen wie dem familiären Mittelmeerfieber (FMF) eine wichtige Rolle. Das S100A12 Protein besitzt drei verschiedene Konformationen: das Dimer, das Tetramer und das Hexamer. In verschiedenen Studien konnte gezeigt werde, dass das Hexamer an proinflammatorische Rezeptoren wie dem Toll-like Rezeptor-4 (TLR-4) und dem „receptor for the advanced glycation end products“ (RAGE) bindet und so die Produktion von weiteren Entzündungsmediatoren stimuliert. Daher besitzt die S100A12 Hexamerkonformation eine entscheidende Rolle in Entzündungsprozessen. Das Ziel bestand somit in der Selektion von Peptiden oder „single chain variable fragment“ (scFv)-Konstrukten, die exklusiv an die hexamere Konformation von S100A12 binden. Mittels Biopanning von Peptid- und scFv-Phagen Bibliotheken konnten Peptide und scFvs selektiert werden. Die selektierten Peptide und die selektierten scFvs wurden in ELISAs weiter auf ihre Bindungseigenschaften charakterisiert. Durch Umklonierung in einen Fc-Konstrukt Vektor konnten die scFvs als vollständige scFv-Fc-Konstrukte exprimiert werden. Die Bindung der selektierten Peptide bestätigte sich als Biotin-Fusion im anschließenden ELISA. Es zeigte sich eine sehr hohe Bindungsspezifität der Peptide und der produzierten scFv-Fc-Konstrukte an das S100A12 Hexamer. Mit den selektierten Liganden ist es gelungen einen Test zu entwickeln: an Streptavidin immobilisierte Peptide binden spezifisch das S100A12 Hexamer aus dem Testmedium und mittels selektiertem scFv-Fc-Konstrukten lassen sich die gebundenen S100A12 Proteine detektieren. Ein Detektionsantikörper ermöglichte die Visualisierung der gebundenen scFv-Fc-Konstrukte mittels Farbreaktion. Das S100A12 Hexamer konnte durch den Testaufbau auch im Plasma spezifisch detekiert werden. Dieser Test könnte es ermöglichen, die exakte Diagnose und vor allem das Überwachung von Patienten mit steigenden Entzündungsmarkern, wie im Rahmen der autoinflammtorischen Erkrankung JIA oder einer Erkrankung wie dem FMF, zu verbessern. Mit einem verbessertem Krankheitsmonitoring könnte ebenfalls die Therapie im frühen Stadium optimiert werden. Zusätzlich könnte ein mögliches therapeutische Potential der S100A12 Hexamer Liganden getestet werden. Sollten die hexamerspezifischen Liganden die Interaktion von S100A12 mit ihren Rezeptoren wie TLR-4 oder RAGE blockieren, ist eine therapeutische Verwendung in der Behandlung von Autoimmun- und autoinflammatorischen Erkrankungen möglich.
Endocrowns: uma Revisão de Literatura
Authors: Silva Jadson Mathyas Domingos da , Souza Karina Barbosa , León Génesis Sulay Alfonzo , Nogueira Júnior Lafayette
Published: August 2023

Introdução: Visando introduzir restaurações mais conservadoras, surgiram as endocrowns, coroas monolíticas cujo preparo envolve às margens da cavidade, retidas pela câmara pulpar sem necessitar do envolvimento dos canais radiculares. Objetivo: Revisar a literatura acerca das endocrowns, de forma a esclarecer suas indicações, vantagens, desvantagens e como elas podem ser utilizadas como alternativa na prática clínica. Métodos: Trata-se de uma revisão integrativa da literatura que consultou à base de dados National Library of Medicine (PubMed), usando-se como descritores: “Endocrowns”, “Endodonticallly treated teeth” e “monoblock”. Os critérios de inclusão para a seleção da amostra foram: artigos publicados e indexados de forma gratuita no período de janeiro de 2012 a janeiro de 2022 (últimos 10 anos). Resultados: Incluiu-se vinte artigos que se enquadraram nos critérios previamente estabelecidos. Os estudos revisados apontam que esta é uma restauração com estética satisfatória e mais conservadora para dentes tratados endodonticamente, sendo uma opção com menor custo e rapidez em suas etapas clínicas, fortemente indicadas para molares, nos casos em que há espaço interoclusal mínimo, bem como a presença de canais radiculares curvos ou estreitos, o que impossibilita a realização do tratamento convencional utilizando pinos radiculares. Conclusões: Endocrowns apresentam-se como opção restauradora viável para molares tratados endodonticamente. Contudo, para pré-molares e dentes anteriores, não há estudos que demonstram efetividade que justifique sua indicação. Estas restaurações podem ser confeccionadas em polímeros e cerâmicas, principalmente as cerâmicas vítreas que favorecem a adesão. Entretanto, mais estudos clínicos precisam ser realizados para melhor compreensão da longevidade desse tipo de restauração
PENGARUH ELECTRONIC WORD OF MOUTH DAN PANIC BUYING TERHADAP KEPUTUSAN PEMBELIAN DI YOGYAKARTA PADA MASA PANDEMI COVID-19
Authors: Setiawan Wahyu , Istriani Ety
Published: August 2023

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh dari electronic word of mouth dan panic buying terhadap keputusan pembelian di Yogyakarta. Teknik pengumpulan data dengan purposive sampling menggunakan google form. Analisis dilakukan menggunakan regresi linear berganda, koefisien regresi, uji t, dan uji f. Responden merupakan masyarakat di Yogyakarta yang melakukan keputusan pembelian pada masa pandemi covid-19. Hasil menunjukkan electronic word of mouth dan panic buying berpengaruh terhadap keputusan pembelian konsumen secara signifikan dan positif, baik secara parsial maupun simultan sehingga seluruh hipotesis terdukung.Kata Kunci: Electronic Word of Mouth, Panic Buying, Keputusan Pembelian, Pandemi Covid-19ABSTRACTThis study aims to determine the effect of electronic word of mouth and panic buying on purchasing decisions in Yogyakarta. The data collection technique was purposive sampling using google form. The analysis was performed using multiple linear regression, regression coefficient, t test, and f test. Respondents are people in Yogyakarta who make purchasing decisions during the COVID-19 pandemic. The results show that electronic word of mouth and panic buying have a significant and positive effect on consumer purchasing decisions, both partially and simultaneously so that all hypotheses are supported. Keyword: Electronic Word of Mouth, Panic Buying, Purchase Decision, Covid-19 Pandemic
ANALISIS PENGARUH KEBIJAKAN MONETER DAN MAKROPRUDENSIAL TERHADAP RISIKO BANK DI INDONESIA
Authors: Silalahi Pristanto , Falianty Telisa Aulia
Published: August 2023

ABSTRAK Tujuan dari penelitian ini adalah untuk menganalisis pengaruh kebijakan moneter dan kebijakan makroprudensial dalam stabilitas perekonomian dengan memitigasi risiko bank di Indonesia. Penelitian ini menggunakan pendekatan z-score untuk mengidentifikasi sekaligus sebagai proksi dari risiko bank. Model data yang digunakan adalah panel data dari tahun 2012 - 2019 dengan sampel 30 Bank. Hasil dari penelitian ini menunjukkan bahwa hampir setiap tahunnya risiko bank meningkat selama periode penelitian dengan metode pengukuran z-score. Hasil dari analisis regresi data panel menunjukkan bahwa kebijakan moneter dengan instrument variabel interest rate (IR) berpengaruh signifikan negatif terhadap risiko bank. Kemudian kebijakan makroprudensial dengan instrument variabel GWM berpengaruh signifikan negatif terhadap risiko bank, hal ini sesuai dengan adanya teori prosiklikalitas di Indonesia. Kata kunci: kebijakan moneter : kebijakan makroprudensial; risiko bank  ABSTRACT The purpose of this study is to analyze the effect of monetary policy and macroprudential policy on economic stability by mitigating bank risk in Indonesia. This study uses a z-score approach to identify as well as a proxy for bank risk. The data model used is panel data from 2012 - to 2019 with a sample of 30 banks. The results of this study indicate that almost every year bank risk increases during the study period using the z-score measurement method. The results of the panel data regression analysis show that monetary policy with the interest rate (IR) variable instrument has a significant negative effect on bank risk. Then macroprudential policy with the variable reserve requirement instrument has a significant negative effect on bank risk, this is under the procyclicality theory in Indonesia. Keywords: monetary policy: macroprudential policy; bank risk
PENGARUH PERSEPSI BUDAYA ORGANISASI DAN MOTIVASI KERJA TERHADAP KINERJA KARYAWAN TOKO LABUAN INDAH NTT
Authors: Subin Klotilda Kurnia , Respati Agustini Dyah
Published: August 2023

ABSTRAK Penelitian ini dilakukan untuk menguji pengaruh budaya organisasi dan motivasi kerja terhadap kinerja karyawan Toko Labuan Indah, Labuan Bajo, Nusa Tenggara Timur. Jumlah responden penelitian 32 karyawan. Metode pengumpulan data yang digunakan adalah kuesioner dan Teknik pengujian data yang dipakai menggunakan analisis regresi linier berganda. Hasil pengujian hipotesis yang dilakukan, didapatkan bahwa budaya organisasi berpengaruh secara signifikan terhadap kinerja karyawan, dan motivasi kerja juga memiliki pengaruh secara signifikan terhadap kinerja karyawan.Kata kunci: budaya organisasi, motivasi kerja, kinerja karyawan ABSTRACT This research was conducted to determine the effect of organizational culture and work motivation on the performance of employees at Labuan Indah Shop, Labuan Bajo, East Nusa Tenggara. The number of research respondents 32 employees. The data collection method used is a questionnaire and the data testing technique used is multiple linear regression analysis. The results of hypothesis testing were carried out, it was found that organizational culture had a significant effect on employee performance, and work motivation also had a significant influence on employee performance.Keywords: organizational culture, work motivation, employee performance
Changes in organic fractions, cations, and stabilization from feces to fecal sludge: implications for dewatering performance and management solutions
Authors: Sam Stanley Bortse , Morgenroth Eberhard , Strande Linda
Published: August 2023

Reliable dewatering performance remains a key challenge in fecal sludge management, and the controlling factors or mechanisms are not well understood. There remain limited studies on constituents in feces and fecal sludge and how they affect the dewaterability of fecal sludge. This study aimed at evaluating a range of constituents in feces, and to gain empirical knowledge toward a mechanistic understanding of how they influence dewaterability. In this study, cellulose reduced capillary suction time, decreased supernatant turbidity, and increased cake solids. While hemicellulose decreased supernatant turbidity, lignin increased supernatant turbidity, capillary suction time, and cake solids. Extracellular polymeric substances (EPS) increased both capillary suction time and supernatant turbidity and decreased cake solids, whereas lipids increased turbidity. Cations had no significant effect on dewatering properties. Overall, fecal sludge stored in containments had better dewatering performance than ‘fresh’ fecal sludge, which was attributed to stabilization. Field fecal sludge had a higher relative abundance of Pseudomonas, which is associated with better aggregation, and fewer small particles (<10 μm) that clog filters to reduce dewatering performance. Further understanding of stabilization and developing an agreed-upon metrics of stabilization are essential for predicting fecal sludge dewatering performance, and developing smaller footprint dewatering treatment technologies.
The Effect of Individual and Regional Characteristics on Commuting (Study on Gerbangkertosusila Area, East Java)
Authors: Muara Setyanti Axellina , Ashar Khusnul , Susilo Susilo
Published: August 2023

Differences in economic opportunity, such as the difference between interregional wage gaps triggered the migration of workers to areas with higher wage rates, as happened in Gerbangkertosusila Area, East Java. In addition to economic factors such as wages, educational factor, age, marital status, unemployment and population number in many theories are also expressed to influence the tendency to migrate. On the other hand the denser urban area gives rise to commuting. Therefore, this study aims to determine the effect of individual and regional characteristics on labor migration in Gerbangkertosusila Area. Using multilevel binary logistic regression, the findings show that the variables significantly affecting commuting decision are age, marital status, secondary and higher education, wage gap and open unemployment rate. Furthermore, workers with a hingher tendency to commute are workers with individual characteristics of male, young, unmarried, highly educated, and self-employed, and with regional characteristics of smaller wage gap, higher open unemployment rate in the origin, and lower population on destination. Keywords Commuting; Gerbangkertosusila Area; Multilevel Logistic; Regional Wage Gap
The Effect of Inflationary Process of Government Revenue and Debt on Inflation in Indonesia: An Ardl Bounds Test Approach
Authors: Kafabih An'im , Maskie Gozalie , Hoetoro Arif
Published: August 2023

The objective of this paper is to examine the cause of inflation based on fiscal policy perspective where government fiscal policy on government revenue and debt is such an inflationary process, meaning that if these two variables do not controlled properly, they will lead to higher level of inflation. Using annual data from 1972 to 2016 and ARDL bounds test approach to process these data, the result of co integration shows that government revenue and debt are simulaneously have long run relationship. This result is supported with the long run and short run relation for each independent variable individually. In the short run and long run, government revenue has positive impact on inflation with lower impact in the long-run compared to the short-run. Furthermore government debt also shows positive impact on inflation both in the long run and short run with the impact in the long run is higher rather that in the short run. As local governments depend heavily on national government on their fiscal resources, this study is also important to local government because any decision of national government will affect to them.
The Nadzir’s Role in Waqf Empowerment for General Welfare (Case Study in the Nadzir of Universitas Islam Malang Foundation)
Authors: Fitria Utami Anisa , Suman Agus , Manzilati Asfi
Published: August 2023

The purpose of this research is to find out the role of the nadzir in developing waqf assets for general welfare. The management of productive waqf for general welfare that conducted by The Nadzir of Univesitas Islam Malang Foundation. The Nadzir of Universitas Islam Malang Foundation received 2 bilion Rupiahs funds from Kementerian Agama that acted as the waqif. The waqf fund are from Anggaran Belanja dan Pendapatan Negara Perubahan with purpose as the pilot program of productive waqf fund management. In order to run the role, The Nadzir of Universitas Islam Malang Foundation is stick with the sharia law and UU No. 41 Tahun 2004. The role of Universitas Islam Malang’s nadzir is suitable with the duties and roles that listed on UU No 41 Tahun 2004. The duties and roles are first, accepting and administrating the waqf asset. Second, managing and developing waqf asset through build VIP Rooms, Al-Khaibar Minimarket and Go Fish Restaurant. Third, keep the waqf asset through pledging the waqf asset to Bank Muamalat and fourth, reporting the waqf asset management to Kementerian Agama as waqif.
A Study on the Vocabulary of Haenyeo Settled in Wando-gun
Authors:   Korean Language & Literature, Kim Gyoung-pyo
Published: August 2023

This study was conducted on a lexical analysis of the vocabulary of Haenyeo settled in Wando-gun. Wando’s settlement Haenyeo used ‘Haenyeo, Jamsu, Jeomnyeo’ like the Jeju Haenyeo, but they also used ‘Bojaegi’, which reveals a negative perception of Haenyeo. And although the names of Haenyeo are ranked as upper, middle, and lower, the hierarchy is not as strict as that of Jeju Haenyeo because there are not many active Haenyeo and they are older. Unlike Jeju Haenyeo, Wando’s settlement Haeyeo used the standard language to name the sea. The vocabulary related to the sea space is named as the name or space of the island that works, unlike Jeju Haenyeo. Wando’s settlement Haeyeo called the work they did in the morning ‘Achim-mule, Achim-mulji’. The vocabulary related to the distance of work was not as subdivided like Jeju Haenyeo and in relation to the space, Wando’s settlement Haeyeo did not use the vocabulary of Jeju Haenyeo when referring to the space. Regarding the act, Wando’s settlement Haenyeo used the ‘Hombes-soli, Sonbi-soli’, which is associated with the ‘Sumbi-soli’ of Jeju Haenyeo. As a collection tool, Wando’s settlement Haenyeo used ‘Mangsali, Mang-ali’ like Jeju Haenyeo and Wando’s settlement Haenyeo used ‘Galgoli, Kkakkuli, Homeng-i’ as a tool for catching octopu and sea urchin, but there was no difference from Jeju Haenyeo’s term. Haenyeo uses ‘Dasdol, Dasjul’ as a secondary tool, but Shinji-myeon and Yaksan-myeon Haenyeo did not use ‘Dasdol, Dasjul’ by changing the method of collecting seafood. Haenyeo also uses ‘Tewag, Duleumbag’ as a secondary tool, and Wando’s settlement Haenyeo said ‘Duleumbag, Duleongbag and Duleombag.’ Wando’s settlement Haenyeo collected ‘Miyeog, Dasima, Mojaban, Tos’ among brown algae. However, unlike Jeju Haenyeo, they used standard language a lot. Shinji-myeon Haenyeo, used ‘Boli-miyeog’ regarding the time of seaweed harvesting. There are also ‘Cheong-gag, Palae’ among green algae, but they did not collect much. Among the red algae were ‘Umu, Cheoncho and Cheonchu.’ Among the mollusks, ‘Sola, Godung, Gunbeos, Gunso, Mun-eo, Jeonbog’ were collected, and among echinoderms, ‘sea urchin and sea cucumber’ were collected. However, unlike Jeju Haenyeo, Wando’s settlement Haenyeo used the standard language. Wando’s settlement Haenyeo were influenced by Jeju Haenyeo and used Jeju Haenyeo’s vocabulary, but also used the Jeonnam dialect in relation to space. However, standard language was used more when talking about the marine environment or harvested products. The reason seems that they used standard language rather than the Jeonnam dialect due to the negative perception of the Haenyeo’s job or the difficulty of settling in the local area. In addition, it seems that standard language has been used more due to various factors such as the influence of broadcasting media, changes in collection methods, and changes in sales methods.
Programa de mejoramiento genético de cacao en Colombia: una propuesta para aunar esfuerzos a nivel nacional en beneficio del productor de cacao colombiano
Authors: Rodríguez-Medina Caren , Sounigo Olivier , Yockteng Benalcázar Roxana , Romero Guerrero Gladys Alejandra , Monsalve García Danilo Augusto
Published: August 2023

Colombia es uno de los países más biodiversos a nivel mundial, cuenta con una amplia diversidad de recursos genéticos de flora y fauna que hacen del país un lugar estratégico, con gran potencial para el desarrollo de programas de mejoramiento genético. En cacao, una especie perenne, el mejoramiento genético es un proceso a largo plazo, que requiere ciclos de evaluación y selección de más de una década; la continuidad de este trabajo por parte de las instituciones involucradas y la intermitencia de las fuentes de financiación, hacen imperante establecer una alianza a nivel nacional que incentive la suma coordinada de esfuerzos del sector público y privado, para garantizar el uso eficiente de los recursos genéticos con los que cuenta el país, reconociendo diferencias regionales que pueden resultar de condiciones socioeconómicas y edafoclimáticas distintas, reflejadas en las diferentes limitantes del sistema productivo en cada región. El objetivo de este libro es socializar el programa actual de mejoramiento genético de cacao propuesto por AGROSAVIA, extendiendo una invitación a otras instituciones nacionales que se encuentran en la actualidad desarrollando actividades de mejoramiento genético, a aunar esfuerzos para construir juntos un programa de mejoramiento genético nacional de cacao que beneficie al productor con materiales de siembra productivos, con resistencia a limitantes fitosanitarias y tolerancia a factores de estrés abiótico. Esta iniciativa responde a la actual política de integración nacional, la cual reconoce diferencias regionales que se capitalizan en la creación de estrategias de articulación, fortaleciendo componentes sociales, económicos y productivos en el país.