bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting and Understanding the Pathology of Single Nucleotide Variants in Human COQ Genes
Authors: Wang Sining , Jain Akash , Novales Noelle Alexa , Nashner Audrey N. , Tran Fiona , Clarke Catherine F.
Published: November 2022

Coenzyme Q (CoQ) is a vital lipid that functions as an electron carrier in the mitochondrial electron transport chain and as a membrane-soluble antioxidant. Deficiencies in CoQ lead to metabolic diseases with a wide range of clinical manifestations. There are currently few treatments that can slow or stop disease progression. Primary CoQ10 deficiency can arise from mutations in any of the COQ genes responsible for CoQ biosynthesis. While many mutations in these genes have been identified, the clinical significance of most of them remains unclear. Here we analyzed the structural and functional impact of 429 human missense single nucleotide variants (SNVs) that give rise to amino acid substitutions in the conserved and functional regions of human genes encoding a high molecular weight complex known as the CoQ synthome (or Complex Q), consisting of the COQ3–COQ7 and COQ9 gene products. Using structures of COQ polypeptides, close homologs, and AlphaFold models, we identified 115 SNVs that are potentially pathogenic. Further biochemical characterizations in model organisms such as Saccharomyces cerevisiae are required to validate the pathogenicity of the identified SNVs. Collectively, our results will provide a resource for clinicians during patient diagnosis and guide therapeutic efforts toward combating primary CoQ10 deficiency.
Bio-Fermented Malic Acid Facilitates the Production of High-Quality Chicken via Enhancing Muscle Antioxidant Capacity of Broilers
Authors: Qiu Kai , He Weizhen , Zhang Haijun , Wang Jing , Qi Guanghai , Guo Naiwei , Zhang Xin , Wu Shugeng
Published: November 2022

Malic acid, an intermediate of the tricarboxylic acid (TCA) cycle, is a promising acidifier with strong antioxidant capacity. This study aimed to evaluate the effects of bio-fermented malic acid (BFMA) on promoting the body health, performance and meat quality of broilers. A total of 288 one-day-old Arbor Acres male broiler chicks were randomly divided into four treatments with six replicates in each. Every replicate had 12 chicks. Four experimental diets contained 0, 4, 8, and 12 g/kg BFMA, respectively. During the 42-day trial, mortality was recorded daily, feed intake and body weight of each replicate being recorded every week. Blood samples were collected on days 21 and 42 for chemical analysis. After slaughter at the age of 42 days, the carcass traits and meat quality of the broilers were measured, breast muscle samples were collected for the determination of antioxidant capacity, and cecal digesta were pretreated for microbiota analysis. Dietary BFMA significantly increased feed intake and daily gain, and decreased feed conversion ratio and death and culling ratio of the broilers at the earlier stage. The water-holding capacity of breast muscle indicated by the indexes of dripping loss and cooking loss was significantly increased by BFMA, especially at the addition level of 8 g/kg. Dietary BFMA significantly decreased the activity of superoxide dismutase and contents of immunoglobulin A and glutathione, and increased contents of immunoglobulin G and M in serum of the broilers. The contents of glutathione, inosinic acid, and total antioxidant capacity and the activities of glutathione-Px and superoxide dismutase were significantly increased by dietary BFMA, with the level of 8 g/kg best. The diversity of cecal microbiota of broilers was obviously altered by BFMA. In conclusion, as one of several acidifiers, addition of BFMA in diets could improve the performance and body health of broilers, probably by reinforcing immunity and perfecting cecal microbiota structure. As one of the intermediates of the TCA cycle, BFMA increases the water-holding capacity of breast muscle of broilers, probably through reducing lactate accumulates and enhancing antioxidant capacity.
Defect Detection for Wear Debris Based on Few-Shot Contrastive Learning
Authors: Li Hang , Li Li , Wang Hongbing
Published: November 2022

In industrial defect detection tasks, the low probability of occurrence of severe industrial defects under normal production conditions has brought a great challenge for data-driven deep learning models that have just a few samples. Contrastive learning based on a sample pair makes it possible to obtain a great number of training samples and learn effective features quickly. In the field of industrial defect detection, the features of some defect instances have small category variance, and the scale of some defect instances has a great diversity. We propose a few-shot object detection network based on contrastive learning and multi-scale feature fusion. Aligned contrastive loss is adopted to increase the instance-level intra-class compactness and inter-class variance, and the misalignment problem is alleviated to a certain extent. A multi-scale fusion module is designed to recognize multi-scale defects by adaptively fusing features from different resolutions with the idea of exploiting the support branch’s information. The robustness and efficiency of the proposed method were evaluated on an industrial wear debris defect dataset and the MS COCO dataset.
Differentiating Human Pluripotent Stem Cells to Cardiomyocytes Using Purified Extracellular Matrix Proteins
Authors: Barnes Ashlynn M. , Holmstoen Tessa B. , Bonham Andrew J. , Rowland Teisha J.
Published: November 2022

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be differentiated into cardiomyocytes (hESC-CMs and iPSC-CMs, respectively), which hold great promise for cardiac regenerative medicine and disease modeling efforts. However, the most widely employed differentiation protocols require undefined substrates that are derived from xenogeneic (animal) products, contaminating resultant hESC- and iPSC-CM cultures with xenogeneic proteins and limiting their clinical applicability. Additionally, typical hESC- and iPSC-CM protocols produce CMs that are significantly contaminated by non-CMs and that are immature, requiring lengthy maturation procedures. In this review, we will summarize recent studies that have investigated the ability of purified extracellular matrix (ECM) proteins to support hESC- and iPSC-CM differentiation, with a focus on commercially available ECM proteins and coatings to make such protocols widely available to researchers. The most promising of the substrates reviewed here include laminin-521 with laminin-221 together or Synthemax (a synthetic vitronectin-based peptide coating), which both resulted in highly pure CM cultures. Future efforts are needed to determine whether combinations of specific purified ECM proteins or derived peptides could further improve CM maturation and culture times, and significantly improve hESC- and iPSC-CM differentiation protocols.
Integrating Armchair, Bench, and Bedside Research for Behavioral Neurology and Neuropsychiatry: Editorial
Authors: Tanaka Masaru , Szabó Ágnes , Vécsei László
Published: November 2022

“To learning much inclined, who went to see the Elephant (though all of them were blind) that each by observation might satisfy the mind” [...]
Characteristics of Placental Histopathology in Women with Uncomplicated Pregnancies Affected by SARS-CoV-2 Infection at the Time of Delivery: A Single-Center Experience
Authors: Sarno Laura , Locci Mariavittoria , Fulgione Caterina , Perillo Francesca , Dell’Isola Angela , Mantelli Dalila , Sibillo Cristina , Saccone Gabriele , Maruotti Giuseppe Maria , Terracciano Daniela , Bifulco Giuseppe , Guida Maurizio , D’Armiento Maria
Published: November 2022

The aim of this study was, firstly, to analyze the histopathological characteristics of placentas in women with uneventful pregnancies and affected by COVID-19 at the time of delivery; and secondly, to correlate histological findings to maternal and neonatal characteristics. In our single-center prospective observational study, 46 placentas from term uncomplicated singleton pregnancies of patients with a documented SARS-CoV-2 infection at the time of delivery underwent histological examination. Despite a normal feto-maternal outcome, most of the placentas (82.6%) presented signs of maternal vascular malperfusion, while features of fetal vascular malperfusion were found in 54% of cases. No correlation was detected between maternal and neonatal characteristics and the severity of blood circulation disease, and abnormal findings were also described in asymptomatic patients. Moreover, we did not find any maternal symptoms or clinical details allowing for the prediction of abnormal placental findings in pregnancy complicated by COVID-19 infection. Our results suggest that SARS-CoV-2 infection during pregnancy could lead to acute placental dysfunction.
Quantification of the Transversal Fiber Strand Stiffness of Textiles Used in Textile-Reinforced Concrete via Shore Hardness
Authors: Beßling Markus , Manko Leonie , Orlowsky Jeanette
Published: November 2022

Textile-reinforced concrete is characterized by its high-performance load-bearing behavior. The basis of these properties is largely determined by the characteristics of the textile used. The textile in turn consists of fibers that are bonded together by means of a matrix (impregnation). Both the fiber material and the impregnation significantly influence the tensile and bonding properties of the textile. The performance of the impregnation depends largely on its stiffness. In this publication, the fiber strand stiffness is quantified by means of shore hardness measurements, and the influence of the fiber strand stiffness on the tensile and composite properties is presented. The Shore hardness is a kind of Young’s modulus. The tests can be performed on the end product (manufactured fiber strand) with little effort. The test setup was adapted to determine the Shore hardness on the fiber strand. A comparison between the hardness and tensile strength shows a direct correlation. A dependency can also be identified and described of the bond between the textile and the concrete and the hardness. The investigations shown make quantifying the fiber strand stiffness based on hardness appear reasonable.
Knowledge of Chinese Pediatric Professionals Regarding Pediatric Pain Management
Authors: Li Zhen-Zhen , Chang Yue-Cune , Gu Lin , Zhou Jian-Fu , Wei Bi-Rong , Peng Niang-Huei
Published: November 2022

Many healthcare professionals base their perceptions of pediatric pain on their knowledge of the subject. Therefore, knowledge deficits in this area may yield negative attitudes toward pain management and add to the complexity of pain management in hospitalized children. This study evaluated the knowledge of pediatric clinicians in China regarding pediatric pain management. Adopting a cross-sectional descriptive comparative design, we surveyed pediatric clinicians using a structured questionnaire. Inclusive criteria were pediatric clinicians, both pediatricians and nurses, with professional pediatric experience of over one year. A total of 507 pediatric clinicians participated. Most were aware of the importance of pain management in sick children but misunderstood pediatric pain, lacked knowledge for performing pediatric pain assessments and lacked knowledge for providing pain relief interventions. Background factors including differing professions (pediatricians and nurses; p = 0.012), age (p < 0.05) and hospital setting of employment (p = 0.003) were significantly related to clinicians’ knowledge regarding pain management. Participating pediatricians had higher levels of knowledge of pediatric pain management than nurses. Research revealed four barriers affecting clinicians’ knowledge, including misconception of pain in children, lack of professional knowledge and confidence in the practice of pediatric pain assessment, lack of professional knowledge to provide pain relief interventions, and a significant knowledge gap between pediatricians and nurses. The results point out a crucial need for multidisciplinary education to remedy these deficiencies. Further study is needed to explore strategies to strengthen clinicians’ knowledge of this vital area of practice.
Management of Juvenile Otosclerosis: A Systematic Review
Authors: Fancello Virginia , Sacchetto Luca , Bianchini Chiara , Ciorba Andrea , Monzani Daniele , Palma Silvia
Published: November 2022

Background. Otosclerosis can occur during childhood, resulting in the early onset of conductive hearing loss. The approach to a child with otosclerosis can present some difficulties in terms of diagnosis and treatment, and the literature on juvenile otosclerosis (JO) is still relatively limited. Aim. To explore the current approaches to JO, in order to clear the management of this condition and evaluate the outcomes and the possible complications of surgical treatment. Methods. A systematic review was performed according to PRISMA guidelines, searching Medline and Embase from January 2002 through to 30 September 2022. A total of 759 papers were identified but based on specified criteria, nine were included in this study. Results. There were 94 children affected by JO and treated by stapes surgery. According to the available data, Male: Female ratio was 1:3–4, whilst the mean ages ranged from 10 to 16.3 years at the time of stapes surgery. After stapes surgery, the target of ABG < 10 dB was achieved in most of the patients. Overall, the 4 complications were reported (4/94= 4%): stenosis of the external ear canal, deterioration of hearing, anacusis with vertigo, tinnitus. Conclusions. The heterogeneity of the available studies does not allow us to draw straight conclusions on this topic, currently. More data about the natural history of the disease in children could help in approaching the treatment correctly, and possibly in drawing guidelines. Studies with a prolonged follow-up could be helpful for assisting clinicians and families in taking the most favorable decision about treatment.
Divergent Trends in the Prevalence of Children’s Asthma, Rhinitis and Atopic Dermatitis and Environmental Influences in the Urban Setting of Zagreb, Croatia
Authors: Topalušić Iva , Stipić Marković Asja , Artuković Marinko , Dodig Slavica , Bucić Lovro , Lugović Mihić Liborija
Published: November 2022

Background: Previous studies have reported that the allergy epidemic in developed countries has reached its plateau, while a rise is expected in developing ones. Our aim was to compare the prevalence of allergic diseases among schoolchildren from the city of Zagreb, Croatia after sixteen years. Methods: Symptoms of asthma, allergic rhinitis (AR) and atopic dermatitis (AD) and risk factors were assessed using the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire. An allergic profile was determined by a skin prick test. Results: The prevalence of current, ever-in-a-lifetime and diagnosed AR of 35.7%, 42.5% and 14.9% and AD of 18.1%, 37.1% and 31.1% demonstrated a significant increase. The asthma prevalence has remained unchanged. The allergen sensitivity rate has remained similar, but pollens have become dominant. Mould and dog exposure are risks for asthma (OR 14.505, OR 2.033). Exposure to cat allergens is protective in AR (OR 0.277). Parental history of allergies is a risk factor in all conditions. Conclusion: Over sixteen years, the prevalence of AR and AD, but not of asthma, have increased. The proportion of atopy has remained high. The AR/AD symptom rise is probably a consequence of increased pollen sensitisation united with high particulate matter concentrations. The stable asthma trend could be a result of decreasing exposures to indoor allergens.
Understanding Italian Consumers’ Perception of Safety in Animal Food Products
Authors: Piochi Maria , Fontefrancesco Michele Filippo , Torri Luisa
Published: November 2022

The concept of food safety is still underexplored among consumers, especially in relationship with the perception of food technology. Through an online survey (n = 489), this study explored: I, how perceived safety is related to products obtained with different technological treatments and described with different commercial information; II, the role of food technology neophobia (FTN) in consumers’ safety perception of animal food products. The technological transformation and commercial information significantly affected the perceived safety in all product categories. Milk and eggs were associated with a high number of perceived hazards (with similar patterns), while honey to the lowest. The certification ‘organic’ positively affected the safety perception of eggs and honey. With the increase of the distance in product origin (local/regional vs. Extra-European) the perceived safety consistently decreased. FTN affected the perceived safety of milk and eggs, depending on the degree of familiarity with the technologies of production. Highly FT neophobic people are perceived as less safe than low FT neophobic people with few familiar products with a higher technological degree of transformation. Results expand the knowledge in people’s attitude towards animal products, particularly considering the technology perception. The outputs may interest policy-makers and food companies, in rethinking the communication strategy concerning food safety.
IUP-BERT: Identification of Umami Peptides Based on BERT Features
Authors: Jiang Liangzhen , Jiang Jici , Wang Xiao , Zhang Yin , Zheng Bowen , Liu Shuqi , Zhang Yiting , Liu Changying , Wan Yan , Xiang Dabing , Lv Zhibin
Published: November 2022

Umami is an important widely-used taste component of food seasoning. Umami peptides are specific structural peptides endowing foods with a favorable umami taste. Laboratory approaches used to identify umami peptides are time-consuming and labor-intensive, which are not feasible for rapid screening. Here, we developed a novel peptide sequence-based umami peptide predictor, namely iUP-BERT, which was based on the deep learning pretrained neural network feature extraction method. After optimization, a single deep representation learning feature encoding method (BERT: bidirectional encoder representations from transformer) in conjugation with the synthetic minority over-sampling technique (SMOTE) and support vector machine (SVM) methods was adopted for model creation to generate predicted probabilistic scores of potential umami peptides. Further extensive empirical experiments on cross-validation and an independent test showed that iUP-BERT outperformed the existing methods with improvements, highlighting its effectiveness and robustness. Finally, an open-access iUP-BERT web server was built. To our knowledge, this is the first efficient sequence-based umami predictor created based on a single deep-learning pretrained neural network feature extraction method. By predicting umami peptides, iUP-BERT can help in further research to improve the palatability of dietary supplements in the future.
Comparative Analysis of Mannans Extraction Processes from Spent Yeast Saccharomyces cerevisiae
Authors: Faustino Margarida , Durão Joana , Pereira Carla F. , Oliveira Ana Sofia , Pereira Joana Odila , Pereira Ana M. , Ferreira Carlos , Pintado Manuela E. , Carvalho Ana P.
Published: November 2022

Mannans are outstanding polysaccharides that have gained exponential interest over the years. These polysaccharides may be extracted from the cell wall of Saccharomyces cerevisiae, and recovered from the brewing or synthetic biology industries, among others. In this work, several extraction processes—physical, chemical and enzymatic—were studied, all aiming to obtain mannans from spent yeast S. cerevisiae. Their performance was evaluated in terms of yield, mannose content and cost. The resultant extracts were characterized in terms of their structure (FT-IR, PXRD and SEM), physicochemical properties (color, molecular weight distribution, sugars, protein, ash and water content) and thermal stability (DSC). The biological properties were assessed through the screening of prebiotic activity in Lactobacillus plantarum and Bifidobacterium animalis. The highest yield (58.82%) was achieved by using an alkaline thermal process, though the correspondent mannose content was low. The extract obtained by autolysis followed by a hydrothermal step resulted in the highest mannose content (59.19%). On the other hand, the extract obtained through the enzymatic hydrolysis displayed the highest prebiotic activity. This comparative study is expected to lay the scientific foundation for the obtention of well-characterized mannans from yeast, which will pave the way for their application in various fields.
Sexual Dimorphism of the Human Scapula: A Geometric Morphometrics Study in Two Portuguese Reference Skeletal Samples
Authors: Maranho Rúben , Ferreira Maria Teresa , Curate Francisco
Published: November 2022

The estimation of biological sex is of paramount relevance in the analysis of skeletal remains recovered in forensic contexts. This study aims to assess sexual dimorphism for identification purposes, from two reference samples of the Portuguese population, and a depiction of the size- and shape-related sexual dimorphism of the human scapula using geometric morphometrics approaches. The sample comprised 211 individuals (100 males and 111 females). A generalized Procrustes analysis (GPA) was performed for shape analyses, a principal component analysis (PCA) and a Procrustes ANOVA were implemented on the GPA transformed variables, and a discriminant analysis was used to assess the cross-validated accuracy of sex estimates. The data showed that male scapulae were larger, with medial and lateral curves more pronounced and an inferior angle more acute than females. The males and females were classified with low accuracy (66.82% and 65.88% for landmarks and semi-landmarks data) based on shape. Combining size and shape variables improved the accuracy of the prediction using landmarks data (80.09%). A combination of both variables might improve the chances of the geometric morphometrics methodology in correctly estimating the sex of unidentified individuals, especially if the skeletal elements show low sexual shape dimorphism.
Changes in the Suicide Rate of Older Adults According to Gender, Age, and Region in South Korea from 2010 to 2017
Authors: Jeong Kyu-Hyoung , Yoon Ji-Yeon , Lee Seoyoon , Cho Sunghwan , Woo Hyun-Jae , Kim Sunghee
Published: November 2022

Background: South Korea’s suicide rates are the highest among Organization for Economic Co-operation and Development (OECD) countries, making it one of the most important societal issues in South Korea. Methods: the statistics on causes of death and resident registration central population (RRCP) provided by the National Statistical Office were used to calculate the suicide rate among older adults in Korea. We examined gender (male, female), age (young–old, old–old), and region (urban, rural) by conducting latent growth modeling to estimate changes in the overall older adult suicide rate and verify its relationship. Results: over a period of 8 years, the older adult suicide rate was 104.232 on average in 2010 and it decreased by approximately 10.317 every year, and the rate of decrease gradually slowed down. The initial value of the older adult suicide rate was found to be higher among males, the old–old group, and those living in rural regions. In the quadratic function change rate, only males and the old–old group were statistically significant. Conclusion: in this study, the direction of and the rate of change in the older adult suicide rates and the relationship between gender, age, and region were examined. It is expected that this study will provide basic data to assist in establishing older adult suicide prevention policies, considering the gender, age, and region of the aging population.
Development of the Self-Perceived Safety of Orthopedic Post-Surgery Inpatients (SPSOPSI) Scale
Authors: Yu Pei-Jung , Tsao Lee-Ing , Liu Chieh-Yu
Published: November 2022

In recent decades, patient safety in orthopedics has gained increasing importance and has been regarded as a core concept of medical care quality. However, according to currently published studies, measurement instruments used to evaluate post-surgery orthopedic patient’s perceptions are still very rare. This study aimed to develop a new measurement instrument, the self-perceived safety of orthopedic post-surgery inpatients (SPSOPSI) scale, which can provide healthcare workers with a better understanding of orthopedic patients’ self-perceived safety and give more precise clinical suggestions. Item analysis and exploratory factor analysis (EFA) were used, and the results showed that the six-factor model is good-fit: root mean square residual (RMR) 0.00, root mean square error of approximation (RMSEA) 0.06, goodness-of-fit index (GFI) 0.90, comparative fit index (CFI) 0.98, incremental fit index (IFI) 0.98. The results showed the SPSOPSI scale is a valid and reliable tool for health care providers can use to evaluate orthopedic post-surgery patients’ perceived safety.
Safety and Efficacy of Tirofiban in Severe Ischemic Stroke Patients Undergoing Mechanical Thrombectomy
Authors: Cai Lingxin , Wang Tingting , Chen Aiqing , Ling Chenhan , Xu Jing , Qian Cong , Chen Gao
Published: November 2022

Tirofiban has recently shown encouraging efficacy and safety among acute ischemic stroke (AIS) patients with mechanical thrombectomy (MT). However, the benefits of tirofiban varied among studies depending on the patient’s condition, which was often not well analyzed. This study aimed to identify the characteristics of patients who may obtain the largest benefits from tirofiban. The efficacy endpoint was a favorable outcome defined as a modified Rankin Scale (mRS) score of 0~2 at 90 days. The safety endpoints were intracranial hemorrhage (ICH), symptomatic intracranial hemorrhage (sICH) and mortality at 90 days. Adjusted logistic regression analysis and subgroup analyses were utilized to investigate the factors associated with tirofiban and the outcome. All of 285 patients fit the inclusion criteria. Tirofiban was associated with a higher rate of favorable outcome (aOR 2.033, 95% CI, 1.002~4.123, p = 0.043) but not with an increased risk of ICH, sICH or mortality (p > 0.05). Moreover, subgroup analyses revealed that tirofiban was associated with favorable outcomes in patients with NIHSS > 14 (aOR 2.778, 95% CI 1.056~7.356, p = 0.038) but not in patients with NIHSS ≤ 14 (aOR 1.719, 95% CI 0.646~4.578, p = 0.278). No significant heterogeneity was found in the effect of tirofiban across the subgroups of age, sex, ASPECTS, time from onset to puncture, use of t-PA or stroke etiology (p for interaction > 0.05). The administration of tirofiban was associated with favorable outcomes in severe ischemic stroke patients, and further studies are needed to confirm this finding.
A Review of Synthetic Image Data and Its Use in Computer Vision
Authors: Man Keith , Chahl Javaan
Published: November 2022

Development of computer vision algorithms using convolutional neural networks and deep learning has necessitated ever greater amounts of annotated and labelled data to produce high performance models. Large, public data sets have been instrumental in pushing forward computer vision by providing the data necessary for training. However, many computer vision applications cannot rely on general image data provided in the available public datasets to train models, instead requiring labelled image data that is not readily available in the public domain on a large scale. At the same time, acquiring such data from the real world can be difficult, costly to obtain, and manual labour intensive to label in large quantities. Because of this, synthetic image data has been pushed to the forefront as a potentially faster and cheaper alternative to collecting and annotating real data. This review provides general overview of types of synthetic image data, as categorised by synthesised output, common methods of synthesising different types of image data, existing applications and logical extensions, performance of synthetic image data in different applications and the associated difficulties in assessing data performance, and areas for further research.
Perception and Quantization Model for Periodic Contour Modifications
Authors: Presnov Dmitri , Kolb Andreas
Published: November 2022

Periodic, wave-like modifications of 2D shape contours are often applied to convey quantitative data via images. However, to the best of our knowledge, there has been no in-depth investigation of the perceptual uniformity and legibility of these kind of approaches. In this paper, we design and perform a user study to evaluate the perception of periodic contour modifications related to their geometry and colour. Based on the study results, we statistically derive a perceptual model, which demonstrates a mainly linear stimulus-to-perception relationship for geometric and colour amplitude and a close-to-quadratic relationship for the respective frequencies, with a rather negligible dependency on the waveform. Furthermore, analyzing the distribution of perceived magnitudes and the overlapping of the respective 50% confidence intervals, we extract distinguishable, visually equidistant quantization levels for each contour-related visual variable. Moreover, we give first insights into the perceptual dependency between amplitude and frequency, and propose a scheme for transferring our model to glyphs with different size, which preserves the distinguishability and the visual equidistance. This work is seen as a first step towards a comprehensive understanding of the perception of periodic contour modifications in image-based visualizations.
The Toxicity of Salicylhydroxamic Acid and Its Effect on the Sensitivity of Ustilaginoidea virens to Azoxystrobin and Pyraclostrobin
Authors: Song Jiehui , Wang Zhiying , Zhang Sijie , Wang Yan , Liang You , Dai Qigen , Huo Zhongyang , Xu Ke
Published: November 2022

Rice false smut (RFS) caused by Ustilaginoidea virens has been one of the most severe rice diseases. Fungicide-based chemical control is a significant measure to control RFS. In the sensitivity determination of quinone outside inhibitor (QoI) fungicide in vitro, salicylhydroxamic acid (SHAM) has been commonly added to artificial culture media in order to inhibit alternative oxidase of phytopathogenic fungi. However, some studies showed that artificial media should not include SHAM due to its toxicity. Whether SHAM should be added in the assay of U. virens sensitivity to QoI fungicide remains unknown. In this study, two appropriate media, potato sucrose agar (PSA) and minimal medium (MM), were selected to test SHAM toxicity and sensitivity of U. virens to azoxystrobin and pyraclostrobin. The mycelial growth and sensitivity to azoxystrobin and pyraclostrobin had no significant difference between on PSA and MM. SHAM could significantly inhibit mycelial growth, conidial germination, peroxidase (POD) and esterase activity of U. virens. Average effective concentration for inhibiting 50% (EC50) values of SHAM against mycelial growth of ten U. virens were 27.41 and 12.75 μg/mL on PSA and MM, respectively. The EC50 values of SHAM against conidial germination of isolates HWD and JS60 were 70.36 and 44.69 μg/mL, respectively. SHAM at 30 μg/mL significantly inhibited POD and esterase activity of isolates HWD and JS60, and even SHAM at 10 μg/mL significantly inhibited POD activity of isolate HWD. In addition, SHAM significantly reduced EC50 values and EC90 values of azoxystrobin and pyraclostrobin on both PSA and MM. Even in the presence of SHAM at 10 μg/mL, average EC50 values of ten U. virens isolates for azoxystrobin decreased 1.7-fold on PSA and 4.8-fold on MM, and for pyraclostrobin that decreased 2.8-fold on PSA and 4.8-fold on MM. Therefore, these results suggest that SHAM should not be included in artificial media in the assay of U. virens sensitivity to QoI fungicides.
Digital Technologies and Public Policies Applied to Green Cities
Authors: Sousa Maria José
Published: November 2022

Digital technologies and public policies are fundamental for cities in defining their urban greening strategies, and the main goal of this research is to identify the applied digital technologies and the public policy dimensions implemented at the national level by the member states to promote urban greening in the literature and official documents. The methodology used is a systematic literature review (based on international studies), a Delphi study with experts, and a policy analysis, aiming to understand how the Portuguese government has implemented policies and identify the main technologies applied to urban greening. The main findings regard (i) the focus on the interaction between actors in policymaking; (ii) interpretive approaches used to examine the application of technologies in urban greening problems; and (iii) how policies reflect the social construction of ‘problems’. The research focuses on how policy analysis provides a powerful tool that can be used to understand the technologies, actions, interests, and political contexts underpinning policy decisions. The main lessons learned from this research are that urban greening can benefit urban centers together with the non-urban environment on which they have a functional impact, such as agricultural hinterland areas, forest spaces around the cities, and the rural–urban interfaces. Initiatives for urban greening are designed to enhance cross-border coordination, complementarities, flexibility, productivity, and access to the main international markets and territories.
Climate Change and Natural Resource Scarcity: A Literature Review on Dry Farming
Authors: di Santo Naomi , Russo Ilaria , Sisto Roberta
Published: November 2022

The agricultural sector is facing the challenge of climate change, which is increasing difficulties to the activity and the economic sustainability of the primary sector, also affecting farmers’ revenues. There is a growing need to support policy makers’ decisions and help them develop cross-sectional strategies to support farmers. To this aim and to collect useful information for policy makers and stakeholders for the development of efficient strategies for the management of dryland farming, the paper examines how this issue has been analysed in the literature. A mixed method, based on a systematic literature review and a bibliometric analysis of 79 Scopus documents using VOSviewer software, was applied. Major results highlight the need to implement participatory policy interventions so as to include farmers. It was possible to summarise the main adaptive and technical interventions implemented by farmers. The results indicated the importance of the concept of the resilience of territories and the need to analyse agricultural systems by considering their multifunctionality. The innovativeness of this study relies on its relationships with several policy aspects and not only with purely technical and agronomical features, analysing thus the issue from the under-investigated perspective of the global challenge, contributing to filling this literature gap.
Mixed-Type Skyrmions in Symmetric Pt/Co/Pt Multilayers at Room Temperature
Authors: He Min , Xu Tiankuo , Gao Yang , Hu Chaoqun , Cai Jianwang , Zhang Ying
Published: November 2022

We demonstrate the generation of mixed-type skyrmions (all are about 200 nm) that are primarily Bloch-type, hybrid-type, and a negligible amount of Néel-type in symmetric Pt/Co(1.55)/Pt multilayers at room temperature. The magnetic field dependence of skyrmion evolution is reversible. Brillouin light-scattering is used to quantitatively quantify the Dzyaloshinskii-Moriya interaction constant D in order to comprehend the mechanism. Interestingly, the D value is high enough to generate skyrmions in a symmetric sandwich structure. Micromagnetic simulations show that Néel-type skyrmions transform into Bloch-type skyrmions as the D value decreases. The interface-induced non-uniform D may be the cause to generate mixed-type skyrmions. This work broadens the flexibility to generate skyrmions by engineering skyrmion-based devices with nominally symmetric multilayers without the requirement of very large DMI.
Fabrication of Ga2O3 Schottky Barrier Diode and Heterojunction Diode by MOCVD
Authors: Jiao Teng , Chen Wei , Li Zhengda , Diao Zhaoti , Dang Xinming , Chen Peiran , Dong Xin , Zhang Yuantao , Zhang Baolin
Published: November 2022

In this article, we reported on a Ga2O3-based Schottky barrier diode and heterojunction diode from MOCVD. The Si-doped n-type Ga2O3 drift layer, grown by MOCVD, exhibited high crystal quality, flat surfaces, and uniform doping. The distribution of unintentional impurities in the films was studied. Then nickel Schottky barrier diode and p-NiO/n-Ga2O3 heterojunction diode were fabricated and measured. Without any electric field management structure, the Schottky barrier diode and heterojunction diode have specific resistances of 3.0 mΩ·cm2 and 6.2 mΩ·cm2, breakdown voltages of 380 V and 740 V, thus yielding power figures of merit of 48 MW·cm−2 and 88 MW·cm−2, respectively. Besides, both devices exhibit a current on/off ratio of more than 1010. This shows the prospect of MOCVD in power device manufacture.
A Survey on Data-Driven Scenario Generation for Automated Vehicle Testing
Authors: Cai Jinkang , Deng Weiwen , Guang Haoran , Wang Ying , Li Jiangkun , Ding Juan
Published: November 2022

Automated driving is a promising tool for reducing traffic accidents. While some companies claim that many cutting-edge automated driving functions have been developed, how to evaluate the safety of automated vehicles remains an open question, which has become a crucial bottleneck. Scenario-based testing has been introduced to test automated vehicles, and much progress has been achieved. While data-driven and knowledge-based approaches are hot research topics, this survey is mainly about Data-Driven Scenario Generation (DDSG) for automated vehicle testing. Rather than describe the contributions of every study respectively, in this survey, methodologies from various studies are anatomized as solutions for several significant problems and compared with each other. This way, scholars and engineers can quickly find state-of-the-art approaches to the issues they might encounter. Furthermore, several critical challenges that might hinder DDSG are described, and responding solutions are presented at the end of this survey.
The Halogen Effect on the Magnetic Behaviour of Dimethylformamide Solvates in [Fe(halide-salEen)2]BPh4
Authors: Marques Rafaela T. , Martins Frederico F. , Bekiş Deniz F. , Vicente Ana I. , Ferreira Liliana P. , Gomes Clara S. B. , Barroso Sónia , Kumar Varun , Garcia Yann , Bandeira Nuno A. G. , Calhorda Maria José , Martinho Paulo N.
Published: November 2022

Complexes [Fe(X-salEen)2]BPh4·DMF, with X = Br (1), Cl (2), and F (3), were crystallised from N,N′-dimethylformamide with the aim of understanding the role of a high boiling point N,N′-dimethylformamide solvate in the spin crossover phenomenon. The counter ion was chosen for only being able to participate in weak intermolecular interactions. The compounds were structurally characterised by single crystal X-ray diffraction. Complex 1 crystallised in the orthorhombic space group P212121, and complexes 2 and 3 in the monoclinic space group P21/n. Even at room temperature, low spin was the predominant form, although complex 2 exhibited the largest proportion of the high-spin species according to both the magnetisation measurements and the Mössbauer spectra. Density Functional Theory calculations were performed both on the periodic solids and on molecular models for complexes 1–3 and the iodide analogue 4. While all approaches reproduced the experimental structures very well, the energy balance between the high-spin and low-spin forms was harder to reproduce, though some calculations pointed to the easier spin crossover of complex 2, as observed. Periodic calculations with the functional PBE led to very similar ΔEHS-LS values for all complexes but showed a preference for the low-spin form. However, the single-point calculations with B3LYP* showed, for the model without solvate, that the Cl complex should undergo spin crossover more easily. The molecular calculations also reflected this fact, which was more clearly defined when the cation–anion–solvate model was used. In the other models there was not much difference between the Cl, Br, and I complexes.
Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)
Authors: Behrendt Undine , Spanner Tobias , Augustin Jürgen , Zak Dominik H. , Horn Marcus A. , Kolb Steffen , Ulrich Andreas
Published: November 2022

Microorganisms acting as sinks for the greenhouse gas nitrous oxide (N2O) are gaining increasing attention in the development of strategies to control N2O emissions. Non-denitrifying N2O reducers are of particular interest because they can provide a real sink without contributing to N2O release. The bacterial strain under investigation (IGB 4-14T), isolated in a mesocosm experiment to study the litter decomposition of Phragmites australis (Cav.), is such an organism. It carries only a nos gene cluster with the sec-dependent Clade II nosZ and is able to consume significant amounts of N2O under anoxic conditions. However, consumption activity is considerably affected by the O2 level. The reduction of N2O was not associated with cell growth, suggesting that no energy is conserved by anaerobic respiration. Therefore, the N2O consumption of strain IGB 4-14T rather serves as an electron sink for metabolism to sustain viability during transient anoxia and/or to detoxify high N2O concentrations. Phylogenetic analysis of 16S rRNA gene similarity revealed that the strain belongs to the genus Flavobacterium. It shares a high similarity in the nos gene cluster composition and the amino acid similarity of the nosZ gene with various type strains of the genus. However, phylogenomic analysis and comparison of overall genome relatedness indices clearly demonstrated a novel species status of strain IGB 4-14T, with Flavobacterium lacus being the most closely related species. Various phenotypic differences supported a demarcation from this species. Based on these results, we proposed a novel species Flavobacterium azooxidireducens sp. nov. (type strain IGB 4-14T = LMG 29709T = DSM 103580T).
Dual Action of Beauveria bassiana (Hypocreales; Cordycipitaceae) Endophytic Stains as Biocontrol Agents against Sucking Pests and Plant Growth Biostimulants on Melon and Strawberry Field Plants
Authors: Mantzoukas Spiridon , Daskalaki Eufrosini , Kitsiou Foteini , Papantzikos Vasileios , Servis Dimitrios , Bitivanos Stergios , Patakioutas George , Eliopoulos Panagiotis A.
Published: November 2022

Entomopathogenic fungi (EPF) can colonize plant tissues and serve crops not only as biopesticides but also as biostimulants that promote plant growth and trigger defense mechanisms. In this context, field trials were conducted evaluating two commercial strains of the entomopathogen Beauveria bassiana (Hypocreales: Cordycipitaceae), GHA (Botanigard) and PPRI 5339 (Velifer® ES) and a wild strain (AP0101) isolated from Achaia, Greece. The three strains were investigated in the field for their endophytic effects on melon Cucumis melo (Cucurbitales: Cucurbitaceae) and strawberry Fragaria sp. (Rosales: Rosaceae) plants and in particular for their ability to colonize plant tissues, control infestations of sucking insects Aphis gossypii (Hemiptera: Aphididae), Chaetosiphon fragaefolii (Hemiptera: Aphididae) and Frankliniella occidentalis (Thysanoptera: Thripidae), and improve plant growth parameters (plant height, number of flowers and fruits). All experimental fungal strains successfully colonized both plants. A significant decrease in the aphid and thrip populations was observed in the treated plants compared to the untreated control. As for plant growth, the number of flowers and fruits was significantly increased in plants treated with B. bassiana strains AP0101 and PPRI 5339. Our results clearly indicate that fungal endophytes can efficiently act as dual action agents demonstrating both insecticidal and growth-promoting effects.
Pollution Dispersion and Predicting Infection Risks in Mobile Public Toilets Based on Measurement and Simulation Data of Indoor Environment
Authors: Li Ruixin , Liu Gaoyi , Xia Yuanli , Bantserova Olga L. , Li Weilin , Zhu Jiayin
Published: November 2022

Since the 21st century, in several public health emergencies that have occurred across the world, the humid enclosed environment of the toilet has become one of the places where bacteria, viruses, and microorganisms breed and spread. Mobile public toilets, as a supplement of urban fixed public toilets, are also widely used in densely populated areas. According to statistics, since the outbreak of COVID-19 in 2019, multiple incidents of people being infected by the COVID-19 virus due to aerosol proliferation in public toilets have been confirmed. It is an urgent issue to resolve the internal environmental pollution of mobile public health and reduce the risk of virus transmission in public spaces under the global epidemic prevention. This paper utilized a typical combined mobile public toilet as the research object and measured and evaluated the indoor thermal environment in real time over a short period of time. The diffusion mode and concentration change law of pollutants in mobile public toilets were predicted and analyzed based on CFD. Regression analysis was also used to clarify the relationship between indoor thermal environment variables and aerosol diffusion paths, and a ventilation optimization scheme was proposed to reduce the risk of virus transmission.
Dynamic Assessment of Cyber Threats in the Field of Insurance
Authors: Pavlík Lukáš , Ficek Martin , Rak Jakub
Published: November 2022

The area of digital technologies is currently the subject of many cyber threats, the frequency of which is increasing. One of the areas of cyber security is also the creation of models and estimates of the process of cyber threats and their possible financial impacts. However, some studies show that cyber-threat assessment to identify potential financial impacts for organizations is a very challenging process. A relatively large problem here is the detection of scenarios of cyber threats and their expression in time. This paper focuses on the design of an algorithm that can be applied to the field of cyber-threat assessment in order to express the financial impacts. The study is based on an in-depth analysis of the insurance industry. The results obtained in our research show the importance of the time perspective for determining the potential financial impacts of cyber threats for the field of insurance.
A Review of Methods Used to Detect Methamphetamine from Indoor Air and Textiles in Confined Spaces
Authors: Kerry Gemma L. , Ross Kirstin E. , Wright Jackie L. , Walker G. Stewart
Published: November 2022

Methamphetamine manufacture, use, and the resulting contamination is a significant issue that affects public health, the environment, and the economy. Third-hand exposure to methamphetamine can result in adverse health risks for individuals and first responders. Such exposures can result from the inhalation of airborne residues or from contact with contaminated objects. This review was conducted to determine the current methods used for methamphetamine extraction from indoor air and porous fabric materials. Dynamic solid phase microextraction (SPME) and sorbent sampling tubes have been applied to extract airborne methamphetamine residues from contaminated properties. SPME and solvent extraction have been applied to sample clothing and textiles for methamphetamine detection. This review demonstrates that there is limited literature on the detection of methamphetamine from indoor air and clothing. Supplementary and consistent methods to detect methamphetamine from air and porous surfaces should be developed and published to allow better assessment of the environmental risk to public health caused by third-hand exposure to methamphetamine.