bluuebunny/crossref_metadata_2025 huggingface dataset:
Koyunlarda Postpartum Dönemde Vaginal Svap Örneklerinden Elde Edilen Bakteri İzolasyonu ve Antibiyogram
Authors: ÜLKER Ufuk , DEMİREL M.ayşe
Published: May 2020

Koyunlarda
uterus, doğum sonrası mikroorganizmaların invazyonuna karşı diğer türlere göre
daha dayanıklı olup bakteriyel eliminasyon hızla gerçekleşebilmektedir.
Uterusun bu özelliğine karşın koyunlarda güç doğum, doğuma yardım girişimleri,
ölü doğum, enfeksiyon kaynaklı abortuslar, uterus prolapsusu ve doğum esnasında
hijyenik olmayan çevresel koşullar postpartum uterus enfeksiyonları için birer
predispoze faktör olmaktadır. Koyunlarda postpartum dönemde uterus enfeksiyonu
ile ilişkili birçok aerob ve anaerob mikroorganizma izole edilebilmektedir.
Araştırmamızda, postpartum 15-30. günlerde 40 adet koyunun vaginal svap
örneklerinden izole edilen bakteri türleri ve bu bakterilere karşı
duyarlı/dirençli antibiyotiklerin belirlenmesi amaçlandı. Steril şartlar
altında alınan svap örnekleri soğuk zincir altında Etlik Veteriner Kontrol
Merkez Araştırma Enstitüsü Mikrobiyoloji Laboratuvarına ulaştırıldı. Yapılan
mikrobiyolojik ekimler sonrası 27 örnekte Escherichia
coli (%71,2), 6 örnekte Stafilokokus
equorum (%15,7) ve 5 örnekte mikst (Escherichia
coli ve Stafilokokus equorum;
%13,1) mikroorganizma izole ve identifiye edilmiş olup izolatların disk
difüzyon yöntemi ile antibiyotik duyarlılıkları belirlenmiştir. Antibiyogram
sonucuna göre Escherichia coli
etkenin ampirik başlanan antibiyotiklere karşı dirençli, dördüncü kuşak
sefalosporin grubu seftazidime ise duyarlı olduğu görüldü. Stafilokokus equorum izole edilen kültürlerde ise geleneksel
tedavide kullanılan antibiyotiklere duyarlı olduğu dikkati çekti. Koyunlarda
postpartum dönemde vaginal mikroorganizma identifikasyonu ve doğru antibiyotik
seçimi reprodüksiyonun devamlılığı için oldukça önemlidir. Bu çalışma ile
koyunlarda postpartum dönemde vaginal svap örneklerinde Stafilokokus equorum izole edildiği ilk kez ortaya konulmuştur.
PENERAPAN METODE PEMBELAJARAN PROBLEM POSING UNTUK MENINGKATKAN KEMAMPUAN SISWA DALAM MEMECAHKAN SOAL CERITA DI SD NEGERI 200010 SIMARDONA
Authors: Siregar Parulian
Published: May 2020

Penelitian ini bertujuan  untuk: (1) mengetahui penerapan metode problem posing untuk memecahkan soal cerita yang mengandung operasi, (2) mengetahui apakah metode problem posing berpengaruh positif terhadap kemampuan siswa dalam memecahkan soal cerita yang mengandung operasi bilangan di SD Negeri 200010 Simardona. Penelitian ini menggunakan pendekatan deskriptif kualitatif  dan kuantitatif. Pengumpulan data dilakukan dengan observasi,  wawancara yang mendalam dan analisis data dengan editing data, reduksi data, deskripsi data, dan penarikan kesimpulan. Sedangkan analisi statistik adalah dengan menggunakan program  windows SPSS 16. Hasil penelitian menunjukkan bahwa: (1) Penerapan metode problem posing dengan tipe presolution posing dalam proses pembelajaran di SD Negeri 200010 Simardona sebagai berikut: (a) membuka pembelajaran dengan baik, (b) menjelaskan langkah-langkah metode problem posing dalam memecahkan soal cerita dengan memberikan contoh, (c) memberikan kesempatan kepada siswa menanyakan hal-hal yang belum dipahami, (d) memfasilitasi kegiatan belajar siswa dengan memberikan Lembar Kerja Pengajuan Masalah (LKPM), (e) mengarahkan dan membimbing siswa dalam menyusun kalimat sederhana menjadi kalimat tanya, pengajuan soal dan cara penyelesaiannya, (f) mengarahkan siswa untuk mengajukan soal yang dibentuknya sendiri untuk dijawab siswa lain, (g) mengarahkan dan membimbing beberapa siswa untuk menanggapi dan menjawab pertanyaan yang diajukan siswa, (h) melakukan umpan balik dan tanggapan terhadap pengajuan masalah yang telah dilakukan, (i) melakukan tanya jawab tentang kesulitan-kesulitan siswa dalam mengajukan masalah, (j) menutup pembelajaran dengan baik. (2) Metode problem posing berpengaruh positif terhadap kemampuan siswa dalam memecahkan soal cerita yang mengandung operasi penjumlahan dan pengurangan bilangan di SD Negeri 200010 Simardona dipengaruhi metode problem posing adalah sebesar 28% dan sisanya dipengaruhi oleh faktor lainnya sebesar 72%.
KONSEP PENDIDIKAN ANAK PADA KELUARGA JAMA’AH TABLIGH
Authors: Zubaidillah Muh. Haris , Nuruddaroini M. Ahim Sulthan
Published: May 2020

Tujuan penelitian ini untuk mengetahui bagaimana konsep pendidikan anak pada keluarga jama’ah tabligh. Metode penelitian ini adalah penelitian kepustakaan (Library Research). Hasil penelitian ini adalah bahwa konsep pendidikan anak pada keluarga jama’ah tabligh meliputi; dasar-dasar pendidikan agama Islam yaitu 13 sifat da’i dan 28 asas dakwah. Program-program meliputi shalat berjama’ah lima waktu di masjid, pendidikan atau pembelajaran Alquran, birrul waa lidain, puasa, taklim dan puasa sunnah. Metode pendidikan agama Islam meliputi, metode kebiasaan, metode keteladanan, metode nasihat, metode monitoring, metode punishment (memberikan hukuman), metode keluar (khuruj). Tujuan pendidikan agama Islam meliputi, untuk mencapai sifat-sifat di antaranya, iman dan yakin seperti iman dan yakinnya Rasulullah saw. Fikir dan risau seperti fikir dan risau Rasulullah saw. Maksud hidup seperti maksud hidup Rasulullah saw. Kecintaan seperti kecintaan Rasulullah saw. Tertib hidup seperti tertib hidup Rasulullah saw, mampu berinteraksi dengan masyarakat, dapat memberikan manfaat kepada orang lain, mampu berdakwah, dan mencari ridha Allah swt dan selalu menjalankan sunnah Nabi. Sedangkan materi pendidikan agama Islam meliputi, ta’lim wa ta’allum berisi beberapa materi, di antaranya adalah materi Alquran, anak-anak mereka di ajarkan cara membaca Alquran, menghafal surah-surah pendek, selain itu juga membacakan kitab fadhilah amal yang materinya tentang fadhilah-fadhilah amal, seperti fadhilah puasa, sedekah, zikir, membaca Alquran dan di bacakan rutin setiap selesai shalat. Selain itu juga materi mengenai enam sifat sahabat, yaitu yakin pada kalimah Thoyyibah, shalat khusu’ dan kudhu’, ilmu dengan zikir, ikramul muslimin, meluruskan niat, dakwah dan tabligh.
POLA ASUH ORANG TUA DALAM MEMBINA KEMAMPUAN MEMBACA AL-QURAN PADA ANAK DI DESA PADANG TANGGUL KECAMATAN AMUNTAI SELATAN
Authors: Mahmud Mahmud
Published: May 2020

Permasalahan yang akan dibahas dalam penelitian ini adalah bagaimana pola asuh orang tua dalam membina kemampuan membaca al-Quran pada anak di Desa Padang Tanggul Kecamatan Amuntai Selatan. Penelitian ini bertujuan untuk mengetahui gambaran yang jelas mengenai bagaimana pola asuh orang tua dalam membina kemampuan membaca al-Quran pada anak di Desa Padang Tanggul Kecamatan Amuntai Selatan. Penelitian ini dilandasi asumsi dasar bahwa orang tua mempunyai peran dan tanggung jawab yang besar dalam membimbing, memberikan perhatian, dan pengawasan bagi anak-anaknya dalam pendidikan membaca al-Quran. Namun sepertinya mereka beranggapan bahwa hal tersebut cukup diserahkan kepada lembaga pendidikan saja. Padahal orang tua seharusnya harus memberikan perhatian, peraturan, pendisiplinan, hadiah dan hukuman agar anak-anaknya mampu membaca al-Quran dengan baik dan benar. Subjek dalam penelitian ini adalah seluruh kepala keluarga yang mempunyai anak usia 5-9 tahun yang berjumlah 58 kepala keluarga dan diambil sampel 8 kepala keluarga secara acak (random sampling) untuk diteliti. Sedangkan objeknya adalah pola asuh orang tua dalam membina kemampuan membaca al-Quran pada anak di Desa Padang Tanggul Kecamatan Amuntai Selatan. Teknik pengumpulan data menggunakan observasi, wawancara dan dokumenter. Data diolah dengan menggunakan teknik reduksi data, display data dan verifikasi data, kemudian diadakan analisis data dengan teknik diskriptif kualitatif dan untuk mengambil kesimpulan digunakan teknik induktif. Hasil penelitian adalah pola asuh orang tua dalam membina kemampuan membaca al-Quran pada anak sudah maksimal. Hal ini disebabkan oleh adanya perhatian yang diberikan orang tua terhadap kemampuan membaca al-Quran kepada anak-anaknya dengan mendidik langsung belajar membaca al-Quran di rumah, adanya peraturan dalam belajar membaca al-Quran yang membuat anak rutin dan disiplin dalam belajar membaca al-Quran, adanya  upaya pendisiplinan dengan memberikan keteladanan kepada anak sehingga menjadikan anak istiqamah dalam belajar membaca al-Quran, adanya pemberian hadiah untuk menambah semangat anak dalam belajar membaca al-Quran dan adanya pemberian hukuman yang bersifat mendidik untuk meluruskan kesalahan anak.
On the origin and evolution of Bacillus anthracis
Authors: Bіlokonov I. I.
Published: May 2020

The paper presents data on the monitoring of the origin and evolution of B. anthracis, which show that the microbe became virulent for humans and animals through the initial transformation into B. cereus group containing a number of closely related species of many spore forming microorganisms inhabiting soil. This was followed by the divergence of B. anthracis from the rest of B. cereus group as a result of obtaining virulence factors such as plasmids рХО1 and рХО2, which determine synthesis of the main virulence factors — the toxin and the capsule. The evolution of Bac. anthracis and the disease caused by the pathogen occurs at the present time as well, possibly even in a reversed direction, as suggested by multiple observations on the circulation of capsule devoid, avirulent forms in animals and in the environment. The new stage in the evolution of Bac. anthracis has started in conjunction with the mass vaccination of animals against anthrax with spore vaccines. In these conditions of vaccination and the presence of active immunity the anthrax bacillus is incapable of infecting an animal, subsequent multiplication, passage to the environment and conversion to the spore form. According to several authors, vegetation of the anthrax microbe in the environmental conditions different from a living organism where the reproduction occurs leads to the loss of virulent properties because they are not required to live in the soil. Other mechanisms of Bac. anthracis evolution cannot be excluded when it resides in the soil, especially at old burial sites where the anthrax bacillus can vegetate during the warm season and to be influenced by action of bacteriophages in the form of genetic transduction, transformation and conjugation. At present, the «anthrax-like» diseases of animals and humans caused by virulent strains of Bac. cereus and Bac. thuringiensis are being registered at increased rate. Diagnosing infectious diseases with clinical and gross-pathological findings of anthrax it is necessary to account the possibility of detection of unusual strains of Bac. anthracis or other bacilli. The correct diagnosis of anthrax can be made only with a complex approach including bacteriological and serological examination, biological assays in laboratory animals, and, essentially, molecular-genetic methods
Research of acute toxicity, allergizing and local-irritative action of the veterinary drug “Yodozol”
Authors: Sachuk R. M. , Zhyhalyuk S. V. , Lukyanik I. M. , Mandyhra M. S. , Stravsky Ya. S. , Katsaraba O. A.
Published: May 2020

The purpose of the work was to determine, in experiments on rodents, the parameters of acute toxicity, allergenic and locally irritative effects of iodine-containing uterine drug for the treatment and prevention of intrauterine infections of animals. Materials and methods. Preclinical studies of acute toxicity of “Yodosol” containing iodine and potassium iodide were performed on 90 white mice, 30 white outbred rats and 6 rabbits. Clinical, pharmacotoxicological and statistical methods were used. Results of work. It has been found that at intragastric administration in experimental rats and mice, DL50 values exceed 8,000 mg/kg body weight and have no effect on the behavioral responses and physiological parameters of laboratory animals. It has been investigated that “Yodosol” aerosol has no local toxic and irritant effects on the skin and mucous membranes of laboratory animals (rabbits). Conclusions. The use of the drug «Yodosol», in doses above 8,000 mg/kg body weight, does not affect the behavioral responses and physiological parameters of laboratory animals. The drug has no local toxic and irritant effects on the skin and mucous membranes. According to the requirements of SOU 85.2-37-736:2011 and GOST 12.1.007-76, the newly developed drug “Yodosol” belongs to low-toxic substances — 4 toxicity classes
Status of metabolic processes in horses during spring period
Authors: Kravchenko N. O. , Kovalenko L. V. , Rudenko O. P. , Boiko V. S.
Published: May 2020

The purpose of these studies was to determine status of metabolic processes in clinically healthy horses of sport breeds in spring period. Blood samples for biochemical studies were collected from 12 clinically healthy 7–9 month-old stallions of Ukrainian horse breed at Dnipropetrovsk region equestrian club. Protein (albumin, globulin, urea and creatinine) and mineral (common calcium and inorganic phosphorus) metabolic statuses, level of glucose, vitamins A and E and acid, as well as activity of hepatospecific enzymes (ALT, AST and AP) were determined using common techniques. It has been found that common protein level was within the limits of physiologic norm, although the level of albumins was decreased at the average rate of 12.0%, and the level of β- and γ-globulins was increased at the average rates of 5.2 and 11.3% respectively. AST activity was decreased at the rate of 38.0% regarding to physiological norm. Thereby, urea and creatinine concentrations were within the referent levels. Hyperglycaemia was observed in 50.0% of tested animals with maximal excess at the rate of 44.0%. Also, decreasing of common calcium and inorganic phosphorus levels was determined at the rate of 16.0 and 58.6%, vitamins А and Е — at the rate of 64.0 and 48,6% respectively, in comparison to lower level of physiological norm. The average index of acid capacity reached maximal referent levels. At the same time, it was increased in 33.3% of animals. Therefore, detected changes in biochemical indices in horse blood evidence that various metabolic disorders progress in clinically healthy stallions at spring and may furtherly lead to the appearance of metabolic syndrome
Stress state and immunological status of young pigs
Authors: Chornyi M. V.
Published: May 2020

The paper presents the results of studies on the effect of regrouping of piglets at different ages on the resistance of their organisms, immunological status, productive qualities and safety of young pigs. The work was performed at “Stas” LLC on large white breed pigs and their crossbreeds. For the experiment, three groups of piglets were formed from suckling sows — analogues. In the experiment 60 animals from birth up to two months of age were used, 20 animals in each experimental group. The control group of piglets was raised from birth up to 60 days of age in nests, and then they were moved to the rearing workshop; Experimental 1 group was kept up to 10 days old in nests, and then a one-time rearrangement was performed according to live weight. Piglets from experimental group 2 were regrouped according to live weight at 5, 10, 15, 21, 30 days of age. To assess the natural resistance of piglets due to the above groups, hematological, biochemical (total protein, protein fractions) methods, immunological (immunoglobulins of classes IgA, IgM, IgG), and natural resistance (bactericidal activity of blood serum) were used, serum lysozyme activity (LASK), phagocytic neutrophil activity (FAN), phagocytic index (FI), ethological, zootechnical, mathematical. To assess the natural resistance of piglets the following research methods were used: generally accepted zoo veterinary methods (live weight, morbidity, safety), hygienic (microclimate conditions, sanitary regime), hematological (morphological blood composition), biochemical (total protein, protein fractions), immunological (immunoglobulins of classes IgA, IgM, IgG), natural resistance (bactericidal activity of blood serum), ethological and mathematical methods. Results of work. It was found that when growing piglets, nesting, without moving, caused their growth and development without any noticeable physiological disturbances, both from the blood and ethology. In animals (Experimental-1 groups) which underwent a single regrouping in a 10 day reward, their lag in live weight by 12.65% was recorded, compared with the control, with two and three-time movement (Experimental-2) — by 16.10%. Their SSPs were 28% less and 14.7% lower than in the control piglets. Patients with symptoms of diarrhea were identified in the control: in 3–4 daily reimbursement — 1%, in Experimental-1 — 5%, Experimental-2 — 10–15%. The resistance of young animals to gastrointestinal diseases according to the Melenberg coefficient in the Experimental-2 group was 6.13–7.48, in control group it was 0.34, and the safety did not exceed 80.2%. According to the level of immune status, animals from Experimental-2 group were inferior: by BASK — by 25.15% (28 days of age), by LASK — by 7.56%, by FAN — by 10.7% compared with peers from Experimental-1 groups. By the number of eosinophils (Experimental-2 group), characterizing the stress state of piglets, their decrease was observed within 10–12 days, not more than 5 days — in animals from Experimental-1 group and up to 3 days — from control
Influence of “Brovadez-plus” disinfectant in different concentrations on Eimeria oocysts in calves
Authors: Bogach N. V. , Skalchuk V. V. , Kushak I. A.
Published: May 2020

The purpose of work was to determine the effect of different concentrations of “Brovadez-plus” disinfectant solution on the sporulation process of mixed culture of eimerias oocysts of calves. Disinvasive activity of the drug “Brovadez-plus” was determined at concentrations of 1.5, 2, 3 and 3.5% for exposures of 3, 5 and 8 hours by irrigation of oocysts. The drug “Brovadez-plus’ at 2% concentration for 3 hours exposure showed an eimeriostatic effect at the level of 29.9 ± 1.4%. At 5 hours exposure, sporogonia did not undergo 51.1 ± 0.5% oocysts, and at 8 hours exposure 64.4 ± 2.2% eimerias oocysts of calves. When using 3% concentration of “Brovadez-plus” solution for 3 hours, sporulation process did not take place in 57.8 ± 0.6% eimerias oocysts, 2.7 ± 0.9% oocysts completed sporulation and 19.6 ± 0.9 % — the sporulation was delayed. The best results were obtained with the use of a 3% solution at an exposure of 5 and 8 hours. The sporogony process did not occur in 96.6 ± 1.2% and 98.2 ± 0.8% of oocysts of eimerias. When using “Brovadez-plus” disinfectant at a concentration of 3.5% at exposure for 3 hours sporogonia did not pass in 78.5 ± 1.1% of eimerias oocysts, at exposure for 5 hours in 96.8 ± 2.1% of eimerias oocysts at exposure 8 hours in 99.1 ± 2.4% of eimerias oocysts of calves. Thus, experimental studies have shown that the drug “Brovadez-plus”, when used in 3% and 3.5% concentrations, has a high level of disinvasive properties relative to eimerias oocysts of calves. Experimental studies have established that the detrimental effect on eimerias oocysts is directly proportional to the duration of their contact and the concentration of the working solution of the studied drug. When using 3% and 3.5% concentrations of “Brovades-plus” disinfectant at an exposure of 5 and 8 hours in 99.1 ± 2.4% of eimerias oocysts of calves, the process of sporogony did not occur
Spread of Pigeon eimeriosis in the conditions of individual farms of the eastern region of Ukraine
Authors: Lyulin P. V.
Published: May 2020

Compliance with veterinary sanitary norms and rules for keeping pigeons does not completely solve the problem of eimeriosis due to the high reproductive ability of the parasite and the long (about a year) preservation of oocysts in the external environment. The aim of the work was to study the epizootic situation regarding the spread of pigeon eimeriosis in individual farms in the eastern region of Ukraine and to determine the species composition of pathogens. Feces of pigeons from individual farms of the eastern region of Ukraine (Kharkiv, Donetsk, Sumy and Poltava regions) served as material for research. 926 samples of pigeon feces were investigated by flotation methods. The species belonging of the causative agents of eimeriosis was determined by the results of own studies of oocyst morphologies and by data from L. P. Pellerdy tables (1974). As a result of studies, it was found that pigeon eimeriosis is widespread in individual farms in the eastern region of Ukraine (prevalence — 52.3%). The smallest eimeriosis invasion was detected in pigeons in Donetsk and Poltava regions, 46.9% and 47.2% respectively, in Kharkov region — 50.2%, and the largest in Sumy region — 65.9%. At the same time, a weak degree of invasion (1–10 oocysts in 1 g of feces) was recorded in 43–64%, medium (11–100 oocysts in 1 g of feces) — 26–45% and strong (> 100 oocysts in 1 g feces) — 10–12% of the number of invaded birds. In the eastern region of Ukraine, three species of Eimeria that cause eimeriosis of pigeons (Eimeria columbae, Eimeria columbarum, Eimeria labbeana) were identified, the ratio of which in the total number of oocysts ranged: Eimeria labbeana — 71.0–81.5%, Eimeria columbarum — 14.5–26.5%, Eimeria columbae — 2.5–4.0%
To the 100th anniversary of Professor Ivan Oleksiyovych Kalashnik — rector of the Kharkiv Zooveterinary Institute (1962–1970)
Authors: Stegniy B. T. , Golovko  , Lyulin P. V.
Published: May 2020

The basic stages of life, scientific and state activity on the way of development and establishment of the Kharkov School of Veterinary Surgeons, the development of the Kharkiv Zooveterinary Institute, the main direction of scientific activity in the development of methods of tissue therapy and biogenic stimulation in animals are highlighted
Analysis and forecasts of Salmonella spp. antibiotic resistance in Dnipropetrovsk region (Ukraine)
Authors: Martynenko H. A.
Published: May 2020

The paper presents data of the research aimed at studying the species composition of major pathogens circulating in the region and the resistance to antibacterial drugs in pathogens of salmonella, one of the most common zoonoses. Within the period 2014–2018 the qualitative composition of microflora from biological and pathological materials from different groups of animals was studied in Dnipropetrovsk region. Own research results and the results of the regional veterinary statistical reporting were analyzed. Within the short period of five years, 237 cultures of Gram-negative bacteria were detected and studied. The dominant agent in the studied region was E. coli (56.7%) from the Enterobacteriaceae family. The second most frequent agent in the pathology was Salmonella spp. (10.5%). In total, 29 cultures of salmonella infection were isolated from six species of animals with a predominance of bird isolates. Thus, for different age groups of poultry the most common were S. Gallinarum-Pullorum (56%) and S. Enteritidis (32%). An antibiotic resistance increase in pathogenic salmonella was observed for β-lactam antibiotics (cefazolin, ceftriaxone), aminoglycosides (gentamicin, streptomycin, kanamycin), as well as for tetracycline and polymyxin. Taking into consideration the high level of resistance against norfloxacin in the region’s dominant pathogens of the Enterobacteriaceae family, we performed a forecast in MS Excel graphically and added a trend line. In the course of work it was proved that the Dnipropetrovsk region is a geographic zone with a stable high (86 ± 3.7%) allocation from different groups of animals of Gram-negative microorganisms. It was found that local dominant pathogens are representatives of the Enterobacteriaceae family (E. coli, Salmonella spp.). This data can be used as surrogate resistance markers. The epizootological patterns of animal salmonellosis are determined. It is shown the possibility of forecasting the distribution of antibiotic resistant strains in MS Excel in graphical form by adding a trend line, using quantitative information on the sensitivity of bacteria. Prospects for further research are the prevention and control of the emergence of resistance to antibiotics in veterinary medicine and agriculture in the region and in the country
Detection of genetic material of Porcine Circovirus type II by loop-mediated isothermal amplification (LAMP)
Authors: Rudova N. G. , Solodiankin O. S. , Gerilovych A. P.
Published: May 2020

There is a sufficient number of molecular-genetic methods for the Porcine Circovirus type II (PCV-II) detection, based on conventional or real-time PCR. However, these methods require expensive equipment, heat cycles for amplification, and considerable time to perform the study. The aim of our work was to develop an alternative method of the PCV-II detection based on isothermal amplification (LAMP), which characterized by cost-effectiveness and short time of study performing. By this reaction a few copies of DNA to 109 molecules might be amplified in about one hour at a constant temperature which is suitable for the field conditions. We designed a set of primers using the target cap gene sequence with the further parameters optimizing of the amplification protocol. Amplification was performed for 60 minutes in a water bath, and the result was observed in UV light using a transilluminator by the adding SYBR green I to the reaction mixture. The elaborated set of primers for LAMP showed high sensitivity and specificity. The set of primers was designed to take into account the molecular genetic features of PCV, and it can significantly expand the range of existing molecular genetic screening techniques for PCV –II detection
Commission testing of the test system “A kit for the detection of antibodies to Newcastle disease virus in hemagglutination inhibition test”
Authors: Stegniy B. T. , Muzyka D. V. , Tkachenko S. V. , Rula O. M. , Stegniy A. B. , Kolesnyk O. S. , Vovk S. I. , Napnenko O. O.
Published: May 2020

Newcastle disease or pseudo-plague is a highly contagious viral bird disease, characterized by damage to the respiratory and digestive organs, as well as impaired central nervous system function. Since the middle of the last century, this disease has become widespread in many European countries. Newcastle disease is common in all continents and is especially dangerous. The article provides information on conducting a round of commission tests of the components of the test system “A Kit for Detection of Antibodies to Newcastle Disease Virus in Hemagglutination Inhibition Test”. When testing encrypted antigens and sera with previously characterized and referent samples, they were active and specific, meeting the requirements of the technical specifications of Ukraine for the specified drug. Thus, the positive antigen had activity in the hemagglutination test of 1:256, reacted only with positive to the Newcastle disease virus serum, and did not delay agglutination in the presence of referent to avian influenza virus subtypes H5 and H7 sera. In the presence of positive sera to the Newcastle disease virus in encrypted form, the positive antigen of the corresponding virus delayed the agglutination of the cock erythrocytes in dilutions 1:256–1:512. The results obtained allowed to make a positive decision on the registration of this preparation in the territory of Ukraine
The study of epizootic sera obtained from ruminant animals in complement fixation test (CFT) with the use of Paratuberculous antigen
Authors: Zavgorodniy A. I. , Pozmogova S. A. , Goncharova N. V. , Kalashnyk M. V. , Bilushko V. V.
Published: May 2020

The paper presents results of the study of epizootic blood sera in the complement fixation test (CFT) with paratuberculous antigen. Blood sera were sampled from the cattle and goats. The antigen was produced from the culture filtrate of Mycobacterium avium subspecies paratuberculosis (MAP) in the laboratory for tuberculosis study. The aim of the present study was to clarify the epizootic situation concerning Johne’s disease among the dairy cattle in different regions of Ukraine. To achieve this aim the blood sera from cattle and goats were collected from farms in different regions of Ukraine. Those sera samples were studied in the complement fixation test with the use of paratuberculous antigen that was produced from the culture filtrate of MAP. The above mentioned blood sera were collected from the cattle that had positive allergic reactions on the use of tuberculin (PPD) for mammals. Those animals belonged to the free from tuberculosis and paratuberulosis milk farms. The study of obtained samples of blood sera was conducted in the accordance with the methodological guidelines “Laboratory diagnostics of paratuberculosis” (shutter. NMR FEFU pr. No. 1, dated December 19, 2014). There were studied 1098 blood sera samples from cattle. In addition to this, investigation was conducted on 24 samples of blood sera from goats. As the result of conducted study it was found that 17 samples of blood sera contained specific antibodies against MAP (serum solution 1:10). These blood sera collected from the cattle belonging to 4 farms in Poltava, Donetsk and Khmelnitsky regions. Along with this it was obtained 9 uncertain results in compliment fixation test that was conducted between paratuberculous antigen (ACF) and blood sera from those 4 farms. The results of monitoring studies indicate that M. avium subsp. paratuberculosis pathogen circulates in studied farms. This can lead to the complication of the epizootic situation regarding paratuberculosis and contribute to the spreading of this pathogen to other free from MAP infection farms. There are no anti-paratuberculosis antibodies in blood serum from goats. It is necessary to conduct annual monitoring serological studies of productive dairy cattle and imported animals in order to clarify and control epizootic situation concerning paratuberculosis on the territory of Ukraine
CLUSTER ANALYSIS OF FACTORS INFLUENCING THE RATIONALITY OF ENTERPRISE POTENTIAL MANAGEMENT
Authors: Shchebel A.
Published: May 2020

The article proves that the management of the potential of the enterprise in market conditions is a complex dynamic phenomenon that can lead to the development of this potential, its reduction or complete disappearance. Capacity development should be understood as its growth and implementation to achieve the goals set by managers. In order for capacity development to take place and have the necessary dynamics for managers, management decisions on the formation and use of enterprise capacity must take into account factors of internal and external environment of the enterprise, in particular those that affect the rationality of management. Factor analysis must be performed permanently. Its results should help business leaders choose sound management decisions. To accomplish this task, factors need to be identified, classified, and evaluated. Identifying factors means identifying them and establishing the relevance of these factors. The classification of factors is necessary for their division into constants and variables, interconnected linearly or indirectly, into significant and insignificant, and so on. The construction of the classification of factors is the result of their evaluation. The study performed a clustering of factors. This made it possible to identify the factors grouped into one group, as well as to identify the nature of the relationships between these factors. The practical application of the proposed analytical and information model of factor consideration in the formation of management decisions to manage the potential of the enterprise will increase the objectivity of the approved decisions in the management of the potential of the enterprise.
Resampling Neural Network Untuk Penanganan Class Imbalance Pada Prediksi Klaim Asuransi
Authors: Hudori Hudori
Published: May 2020

Algoritma Neural Network memiliki kelebihan akan tingkat akurasi perhitungan yang lebih baik dari algoritma lain karena proses perhitungan dilakukan berulang-ulang sehingga membutuhkan waktu yang lebih lama dalam memproses atau men-training data. Namun algoritma ini juga sangat sensitive terhadap dataset yang memiliki class yang sangat tidak seimbang. Penanganan ketidakseimbangan kelas (class imbalance) yang terjadi pada dataset dapat diatasi dengan teknik resampling. Tulisan ini membandingkan tiga metode resampling untuk menangani ketidak-seimbangan class yang diterapkan pada algoritma Neural Network sebagai salah satu algoritma data mining untuk prediksi Claim Kecelakaan Diri Penumpang Berdasarkan Karakteristik Kendaraan Tertanggung. Metode ini dibangun dan diuji menggunakan data transaksi yang real dari sebuah perusahaan asuransi terkemuka di dunia yang meminta sebuah perusahaan crowdsourching untuk menyelenggarakan kompetisi pembangunan model prediksi Claim Kecelakaan Diri Penumpang Berdasarkan Karakteristik Kendaraan Tertanggung. Pada umumnya data transaksi, data ini juga masih memiliki sifat berdimensi tinggi, heterogen dan nilai kosong pada beberapa variable.
PERGURUAN THAWALIB PADANG PANJANG IN THE PERSPECTIVE OF EDUCATIONAL HISTORY 1912 - 1926
Authors: Harmonedi Harmonedi
Published: May 2020

Perguruan Thawalib Padang Panjang has contributed greatly to the nation. The history of its establishment cannot be separated from Surau Jembatan Besi. To uncover this problem the authors conducted research under the title " Perguruan Thawalib Padang Panjang in the Perspective of Educational History 1912-1926". This research aims at revealing the history of Perguruan Thawalib Padang Panjang, and its work in education. it is qualitative research through library studies. After conducting research, it was revealed that Surau Jembatan Besi, is used to implement the traditional education system, turned into Thawalib Padang Panjang, It implements a modern education system. The modernization of education is motivated by the demands of the people who need a noble, intelligent, critical, skilled generation. The renewal efforts carried out is to encourage the students with critical thinking, independent in opinion and skilled the organization, implementing classical system education, establishing teacher handbooks, and developing curriculum. The main figure in the modernization of education in Thawalib Padang Panjang is Sheikh Abdul Karim Amrullah, a charismatic cleric who has been in touch with modernization movements in the Middle East.
TRADISI SURAT MENYURAT SULTAN INDRAPURA DENGAN DEPATI KERINCI
Authors: ZE DEKI SYAPUTRA
Published: May 2020

Salah satu manuskrip yang banyak menjadi pusat perhatian para peneliti/pengkaji adalah surat kerajaan/kesultanan, selain karena surat merupakan manuskrip terawal yang dihasilkan oleh masyarakat masa lalu juga dikarenakan surat memiliki struktur tetentu dalam penulisannya. Banyak peneliti/pengkaji yang pernah menjadikan surat sebagai objek penelitian/pengkjiannya seperti halnya Gallop membahas tentang struktur surat menyurat di dunia Melayu mulai dari reka bentuk dan hiasan sampai dengan adat penggiring surat. Oleh karena itu,  penulis tertarik dengan hal ini untuk melihat tradisi yang diterapkan oleh pihak Kesultanan Indrapura dalam surat menyurat khususnya dalam mengirim surat ke para depati di Kerinci. Metode yang digunakan dalam penelitian ini adalah metode kualitatif yang bertujuan untuk mengumpulkan data, menganalisis data dan perumusan. Sedangkan untuk memahami isi naskah surat, pemaknaan terhadap teks dan konten naskah menggunakan pendekatan filologi dan kodikologi. Dari naskah surat keterangan Marah Muhammad Baki gelar Tunku Sultan Firmansyah kepada Kyai Depati Empat Pemangku Lima Nan Selapan Helai Kain di dalam Alam Kerinci pada tanggal 29 Mei 1888 M, dapat diketahui tradisi surat menyurat baik struktur surat maupun adat laluan dari surat tersebut. Hal yang menarik dari tradisi tersebut adalah waktu rentang waktu pembacaan surat dari waktu surat tersebut sampai sangat lama sekali hingga sekitar tiga hari tiga malam, karena harus mengumpulkan seluruh depati di Alam Kerinci yang sesuai dengan adat purbakala.
PERKEMBANGAN TARI MERAWAI DI PULAU LIPAN KABUPATEN LINGGA
Authors: ARMAN DEDI
Published: May 2020

Tari merawai merupakan tarian yang hampir punah milik Orang Laut yang ada di Pulau Lipan, Desa Penuba, Kecamatan Selayar, Kabupaten Lingga. Tarian ini seakan hilang di Pulau Lipan dan baru kembali ditampilkan tahun 2018 lalu.  Fokus tulisan ini dua hal, yakni perkembangan tari  merawai di Pulau Lipan, Lingga dan  faktor-faktor yang menyebabkan tari merawai terancam punah. Penelitian ini adalah penelitian sejarah.  Teknik pengumpulan data adalah studi pustaka, observasi dan wawancara. Temuan tulisan ini menunjukkan tarian merawai berasal dari Pulau Lipan dan tidak ditemukan di daerah lainnya di Kabupaten Lingga. Pada periode tahun 1950-an sampai periode tahun 1990-an, tari merawai sering ditampilkan Orang Laut dalam acara  keramaian. Setelah era reformasi, tari merawai makin jarang ditampilkan Orang Laut. Dalam perkembangannya, tari merawai ditampilkan sanggar-sanggar seni yang ada di Kabupaten Lingga dalam event kesenian, tetapi personilnya bukan Orang Laut. Tari merawai yang ditampikan juga sudah tari kreasi. Sejumlah pelaku tari merawai di Pulau Lipan masih ada namun pewarisan tari merawai juga tidak berjalan. Generasi muda Orang Laut lebih tertarik dengan kesenian modern.
REPRESENTASI KUCING DALAM FOKLOR SUNDA
Authors: ROSTIYATI ANI
Published: May 2020

Penggambaran kedekatan kucing terdapat dalam berbagai folklor di berbagai belahan dunia. Pada masyarakat Sunda, kedekatan tersebut direpresentasikan dalam folklor lisan Nini Anteh  dan permainan tradisional anak. Namun, kajian mendalam tentang keterkaitan keberadaan kucing dalam folklor lisan cerita rakyat Nini Anteh dan permainan tradisional anak belum pernah dilakukan. Oleh sebab itu, tujuan kajian ini adalah mengungkap representasi kucing dalam foklor lisan tersebut. Adapun metode penelitian adalah deskriptif-kualitatif dengan pengambilan data melalui studi pustaka serta wawancara mendalam pada informan yang dianggap memiliki enkulturasi penuh. Data yang telah diperoleh  kemudian dianalisis meliputi alur, makna, dan fungsinya. Hasil dari analisa tersebut diperoleh bahwa  kata  kucing dalam permainan tradisional anak merepresentasikan identitas budaya lokal dan kolektif bagi masyarakat Sunda serta media pendidikan bagi anak. Sedangkan kucing dalam cerita rakyat Nini Anteh dipandang sebagai subjek yang penting sebagai representasi dari domestikasi Nini Anteh yang menempatkan perempuan sebagai subordinat dari laki-laki dan menimbulkan ketimpangan sosial berbasis identitas gender.
DAMPAK LINGKUNGAN TERHADAP PERKEMBANGAN KEPRIBADIAN TOKOH AKU DALAM NOVEL SEMUSIM, DAN SEMUSIM LAGI KARYA ANDINA DWIFATMA: PERSPEKTIF URI BRONFENBRENNER
Authors: Fibiani Mufadila , Andalas Eggy Fajar
Published: May 2020

ABSTRAKOrang tua merupakan lingkungan pertama yang berperan besar terhadap perkembangan kepribadian anak sehingga kepribadian anak merupakan cerminan dari pola asuh orang tuanya. Penelitian ini bertujuan untuk mendeskripsikan dampak lingkungan terhadap perkembangan kepribadian tokoh aku dalam novel Semusim, dan Semusim Lagi karya Andina Dwifatma. Penelitian ini merupakan penelitian kualitatif deskriptif analitik dengan menggunakan pendekatan psikologi sastra. Sumber data penelitian berupa novel Semusim, dan Semusim Lagi karya Andina Dwifatma. Proses pengumpulan data dilakukan dengan membaca dan mengoding. Analisis data dilakukan dengan tahap 1) display, 2) reduksi, dan 3) penarikan simpulan (Milles dan Huberman). Hasil penelitian menunjukkan bahwa lingkungan berdampak besar terhadap perkembangan kepribadian anak. Dampak tersebut dibagi menjadi empat, yaitu mikrosistem, mesosistem, eksosistem, dan makrosistem.Kata kunci: dampak lingkungan, perkembangan kepribadian, psikologi sastraABSTRACTParents are the first environment that plays a major role in the development of a child's personality so that the child's personality is a reflection of his parents' parenting. This research aimed to describe the environmental impact towards the personality development of I in Semusim, dan Semusim Lagi written by Andina Dwifatma. This is a descriptive qualitative analytic research with psychology of literature approach. The data source is the Semusim, dan Semusim Lagi, a novel written by Andina Dwifatma. The data collection is done through close reading and coding. The data analysis is done through 1) display, 2) reduction, and 3) conclusion (Milles and Huberman). The research result showed that the parenting style has a big impact into the childrenâ€™s personality development. The impact is divided into 4 environments is microsystem, mesosystem, ecosystem, and macrosystem. Keywords: environmental impact, personality development, psychology of literature
ANALISIS NILAI-NILAI RELIGIUS DALAM NOVEL MENCINTAIMU SEPERTI KUCINTAI QURâ€™AN KARYA WAHYU SUJANI
Authors: Mulyati Mulyati
Published: May 2020

ABSTRAKNovel merupakan karya fiksi imajinatifÂ  ekspresi pengarang tentang fenomena kehidupan manusia Â Â Â yang mengandung unsur ekstrinsik. Salah satu unsur ekstrinsik tersebut adalah nilai-nilai religius. Novel Mencintaimu Seperti Kucintai Qurâ€™an adalah salah satu ekspresi Â Wahyu Sujani yang memiliki nilai-nilai religius yang kental. Â Penelitian ini bertujuan mendeskripsikan nilai-nilai religius tersebut melalui teknik dokumentasi dan analisis karya melalui pendekatan pragmatik. Data yang dianalisis bersumber dari novel Â Mencintaimu Seperti Kucintai Qurâ€™an karya Wahyu Sujani yang menjadi pusat kajian. Hasil analisis nilai religius yang ditemukan dalam novel Mencintaimu Seperti Kucintai Qurâ€™an Â karya Wahyu Sujani adalah: 1) Hubungan manusia dengan Allah (beribadah kepada-Nya, mensyukuri nikmat-Nya, bersabar menerima cobaan Allah, dan memohon ampun dan taubat kepada Allah);Â  2) Hubungan manusia dengan hati nurani atau diri sendiri (sabar, memegang amanah, pemaaf,Â  ikhlas); 3) Hubungan manusia dengan sesama manusia (tolong menolong, menegakkan keadilan); dan 4) Hubungan manusia dengan lingkungan hidup (tumbuh-tumbuhan).Kata kunci: analisis, nilai religius, novelABSTRACTThe novel is an imaginative work of fiction author expression about the phenomenon of human life that contains extrinsic elements. One of the extrinsic elements is religious values. The Novel Mencintaimu Seperti Kucintai Qurâ€™an is one of the revelations of Wahyu Sujani which has thick religious values. This study aims to describe these religious values through documentation techniques and analysis of works through a pragmatic approach. The data analyzed were sourced from the novel Mencintaimu Seperti Kucintai Qurâ€™an by Wahyu Sujani, which became the center of the study. The results of the analysis of religious values found in this novel are: 1) relationship with God (worshiping Him, giving thanks for His blessings, being patient in accepting God's trials, and asking God for forgiveness and repentance); 2) Human relationship with conscience or self (patient, holding trust, forgiving, sincere); 3) Human relations with fellow human beings (please help, uphold justice); and 4) The relationship between humans and the environment (plants).Keywords: analysis,, religious values, novel
MANIPULASI TANDA DALAM CERPEN â€œBERTENGKAR BERBISIKâ€ KARYA M. KASIM: TINJAUAN SIMULAKRA JEAN BAUDRILLARD
Authors: Himmah Aliyatul
Published: May 2020

ABSTRAKArtikel ini mengkaji cerpen â€œBertengkar Berbisikâ€ yang ditulis oleh M. Kasim dengan menerapkan konsep simulakra oleh Jean Baudrillard. Simulakra merupakan sebuah upaya untuk memanipulasi tanda agar makna yang dimanipulasi tersebut tampak nyata dan dianggap sebuah realitas sejati oleh masyarakat. Simulakra dicirikan dengan adanya dua hal, yakni hiperrealitas dan simulasi. Berdasarkan hal itu, ditemukan sebuah upaya manipulasi tanda yang dilakukan oleh tiga tokoh yang berperan sebagai musafir dalam cerpen. Motif yang dilakukan melalui tindakan ini adalah keinginan tiga tokoh akan kemudahan akses kepada kepala kampung agar mereka dapat diterima dengan baik. Semula hiperrealitas serta simulasi sebagai komponen dari simulakra, yang dibangun oleh ketiga tokoh ini bekerja dengan apik hingga mereka sendiri menghancurkan bangunan tersebut.Kata kunci: bertengkar berbisik, hiperrealitas, simulakra, manipulasi tanda, konsumsiÂ ABSTRACTThis article examines â€œBertengkar Berbisikâ€ short story by M. Kasim applying the concept of simulcrum by Jean Baudrillard. Simulacrum is an attempt to manipulate a sign so that the manipulated meaning appears real and is considered a true reality by the community. Simulacrum is characterized by two components, hyperreality and simulation. Through this theory, it is seen that an attempt was made to manipulate the signs carried out by the three figures who acted as travelers in â€œBertengkar Berbisikâ€ short story. The motive carried out by the three figures is their desire to have easy access to the head of the village in order to be well accepted. In the beginning, hyperreality and simulation, as components of simulacrum, built by these three figures worked successfully. However, they temselves consciously destroy the building so that their manipulated sign is known by the society.Keywords: â€œBertengkar Berbisikâ€, hyperreality, simulacrum, manipulation of signs, consumption
METAFORA SEKSUAL DALAM SERAT CENTHINI SEBAGAI WUJUD REPRESENTASI AJARAN KESEMPURNAAN HIDUP MASYARAKAT JAWA
Authors: Nurnaningsih Nurnaningsih
Published: May 2020

ABSTRAKMetafora seks dalam Serat Centhini termasuk bahasa figuratif. Bahasa sebenarnya sebuah tanda yang mencerminkan pola pikir, pandangan hidup atau pandangan dunia masyarakatnya. Tujuan penelitian ini adalah menemukan ajaran kesempurnaan hidup di balik metafora seks dalam Serat Centhini. Jenis penelitian ini adalah penelitian kualitatif. Metode penelitian kualitatif deskriptif. Teori yang digunakan dalam penelitian ini adalah teori mitologi Roland Barthes tentang signifikasi makna. Sumber data dalam penelitian ini adalah Serat Centhini berupa tulisan Latin terdiri dari 12 jilid diterbitkan oleh Karkono Kamajaya melalui Yayasan Centhini tahun 1986 dan narasumber. Datanya berupa data kebahasaan yang berwujud larik yang ada berikut metaforiknya, terutama yang mengandung metafora seksual dan transliterasi hasil wawancara. Hasil penelitian ditemukan bahwa ajaran seks dalam SC yang diwujudkan dengan gaya bahasa metafora sebenarnya mencerminkan pandangan hidup masyarakat Jawa. Manusia hendaknya selalu sadar akan bibit kawite 'asal mulanya' dan harus berupaya memaknai makna hidup ini untuk menuju kesempurnaan hidup atau manunggaling kawula Gusti. Konsep ini dalam SC sering diungkapkan dengan bahasa yang arkais, indah, dan santun.Kata kunci: metafora seksual, Serat Centhini, ajaran hidupÂ ABSTRACTÂ Sex metaphor in Serat Centhini (SC) is included inÂ  figurative language. Language is actually a sign that reflects the mindset, outlook on life or world view of the people. The purpose of this study is to find the teachings of the perfection of life behind the sex metaphor in SC. This type of research is qualitative research and the method used is descriptive qualitative. The theory used in this research is Roland Barthes's mythology about the significance of meaning. The data source in this research is SC in the Latin writing consists of 12 volumes published by Karkono Kamajaya through Centhini Foundation in 1986 and information from some Â informants. The data is linguistic data in the form of an array and its metaphorics, especially that is containing the metaphors of sexual tools and the results of interviews. Sexual intercourse taught in SC with metaphorical language style reminds that humans should always be aware of bibit kawite â€˜the origin of marriage'Â  and must try to interpret the meaning of life in order to achieve the perfection of life â€˜manunggaling kawula Gusti.â€™ This concept in SC is often expressed in an archaic, beautiful, and polite language.Keywords: sexual metaphors, Serat Centhini, teachings of life