Isolation of megakaryocytes using magnetic cell separation and adverse effects induced by diclofenac toxicity in an experiment
Authors: Bagmut Irina Yuriivna ,   Department of Clinical Pathophysiology, Topographic Anatomy and Operative Surgery, Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine,   Irina Yuriivna Bagmut, Department of Clinical Pathophysiology, Topographic Anatomy and Operative Surgery, Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine. E-mail: irina.bagmut@ukr.net, Ivanov Olexiy Sergiyovych ,   Department of Clinical Pathophysiology, Topographic Anatomy and Operative Surgery, Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine, Sheremet Michael Ivanovich ,   Surgery Department No1, Bukovinian State Medical University, Chernivtsi, Ukraine,   Michael Ivanovich Sheremet, Surgery Department No. 1, Bukovinian State Medical University, Chernivtsi, Ukraine. E-mail: mihayl71@gmail.com, Smirnov Sergiy Mykolayovych ,   Department of Medical Biology, Histology and Cytology, Lugansk State Medical University, Rubizne, Ukraine, Kolisnyk Igor Leonidovich ,   Department of Clinical Pathophysiology, Topographic Anatomy and Operative Surgery, Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine, Ivanova Julia Viktorivna ,   Zaytsev V.T. Institute of General and Urgent Surgery of National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine, Tymchenko Mykhailo Yevgenievich ,   Department of Comprehensive Programming for the Development of Urgent Surgery and Intellectual Property Protection, SI ZIGUS NAMSU, Kharkiv, Ukraine, Oleksievich Lazirskiy Vyacheslav ,   Department of Surgery No. 1, Kharkiv National Medical University, Kharkiv, Ukraine
Published: November 2022

This study investigates the response of bone marrow (particularly megakaryocytes) in mice under the influence of diclofenac sodium for 10 days using intraperitoneal injection at various doses. A fundamentally new immunomagnetic separation method was applied during the experiment, which helped obtain pure lines of bone marrow cells, particularly megakaryocytes (MC), without admixtures of other cells or their particles. The resulting cells completely retain their structure and can be used in further research. The study determined that different doses of diclofenac sodium have different effects on different groups of diabetes mellitus cells CD34-megakaryocytes. The use of 1.0 mg/ml sharply negatively affects the state of early populations of megakaryocytes (decrease by 80%, p=0.05), a dose of 0.025 mg/ml had the least effect on this population of cells (22.8%, p=0.05). The greatest number of average forms of diabetes mellitus 34 was observed when using a dose of 0.95 mg/ml (22.8%, p=0.05), with a gradual decrease in the dose, the indicator of this group of cells decreased. A dose of 0.03 mg/ml did not affect the quantitative state of megakaryocytes, and a dose of 0.025 mg/ml caused a slight decrease (16.6%, p=0.05). Indicators of mature cells of megakaryocytes CD 34- decreased in all studied groups, however, their maximum value reached a maximum decrease by 0.25 mg/ml (55.2%, p=0.05), the dose of diclofenac sodium 0.03 mg/ml, lower (18.4%, p=0.05). Diclofenac sodium in different doses has different effects on the degree of differentiation of CD 34-. Its introduction positively affects the state of intermediate forms of megakaryocytes, except for minimal doses, while the effect on early and mature forms in all cases turned out to be negative.