bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation of novel simian adenoviruses from macaques for development of a vector for human gene therapy and vaccines
Authors: Lan Wendong , Quan Lulu , Li Yiqiang , Ou Junxian , Duan Biyan , Mei Ting , Tan Xiao , Chen Weiwei , Feng Liqiang , Wan Chengsong , Zhao Wei , Chodosh James , Seto Donald , Zhang Qiwei
Published: September 2023

Both human and non-human simian adenoviruses (HAdVs and SAdVs, respectively) have been used as gene therapy and vaccine vectors. The high prevalence of HAdVs and the neutralizing antibodies associated with prior infection, may limit HAdV-based vector use in human subjects. To overcome this drawback, a vector derived from a newly isolated and characterized macaque adenovirus was constructed. SAdVs (33.9%) were screened from 115 SAdV fecal samples collected at a zoological park. One novel SAdV was isolated and the whole genome was sequenced and analyzed. The pre-existing neutralizing antibody levels were very low against this isolate (10%). Interestingly, SAdV vector constructs that lack E3 region could not produce infectious progeny in HEK293 cells, suggesting that the E3 region is necessary for SAdV replication. The absence of E3 region could be compensated for by replacement with HAdV-5 E4orf6; the resultant construct could replicate well in HEK293 cells. The enhanced Green Fluorescent Protein (eGFP) was inserted into SAdV E3 region and expressed at high level. One-step growth curve showed that the replication of the SAdVs with HAdV-5 E4orf6 substitution and E1/E3 deletion was similar to that of wild-type SAdVs in HEK293 cells, but the modified SAdVs were replication-deficient in A549 cells which lack HAdV-5 E1A and E1B. Finally, we demonstrated that GZ3-12 could infect cells expressing hCAR or hDSG2 receptors. The successful isolation, characterization, and modification of novel SAdVs provide a potentially important vaccine and gene therapy candidate and a new strategy for the rapid acquisition and development of non-HAdV-based alternative vectors for human health applications.

IMPORTANCE

Adenoviruses are widely used in gene therapy and vaccine delivery. Due to the high prevalence of human adenoviruses (HAdVs), the pre-existing immunity against HAdVs in humans is common, which limits the wide and repetitive use of HAdV vectors. In contrast, the pre-existing immunity against simian adenoviruses (SAdVs) is low in humans. Therefore, we performed epidemiological investigations of SAdVs in simians and found that the SAdV prevalence was as high as 33.9%. The whole-genome sequencing and sequence analysis showed SAdV diversity and possible cross species transmission. One isolate with low level of pre-existing neutralizing antibodies in humans was used to construct replication-deficient SAdV vectors with E4orf6 substitution and E1/E3 deletion. Interestingly, we found that the E3 region plays a critical role in its replication in human cells, but the absence of this region could be compensated for by the E4orf6 from HAdV-5 and the E1 expression intrinsic to HEK293 cells.
Retrospective identification of pathogenic mycobacterial species in fish:
Mycobacterium pseudoshottsii
YM-3, isolated from a yellowtail fish in 1986 in Kochi, Japan
Authors: Imajoh Masayuki , Yoshida Shiomi , Nonaka Lisa , Fukushima Yukari , Nakajima Chie , Suzuki Yasuhiko , Wada Takayuki
Published: September 2023

The complete genome sequence of mycobacterial strain YM-3, isolated from cultured yellowtail in 1986, was determined. The strain was
Mycobacterium pseudoshottsii
, a closely related subspecies of
Mycobacterium marinum
, so the strain was isolated earlier than the first report of the subspecies in 2005.
Genome sequences of key bacterial symbionts of entomopathogenic nematodes:
Xenorhabdus cabanillasii
DSM17905,
Xenorhabdus ehlersii
DSM16337,
Xenorhabdus japonica
DSM16522,
Xenorhabdus koppenhoeferii
DSM18168, and
Xenorhabdus mauleonii
DSM17908
Authors: Robertson Raegan , Conrad Katie , Ahuja Baarik , Göker Markus , Hahnke Richard L. , Spunde Alex , Ivanova Natalia N. , Seshadri Rekha , Stephens Craig
Published: September 2023

Xenorhabdus
species are bacterial symbionts of entomopathogenic
Steinernema
nematodes, in which they produce diverse secondary metabolites implicated in pathogenesis. To expand resources for natural product prospecting and exploration of host-symbiont-pathogen relationships, the genomes of
Xenorhabdus cabanillasi, Xenorhabdus ehlersii
,
Xenorhabdus japonica
,
Xenorhabdus koppenhoeferii
, and
Xenorhabdus mauleonii
were analyzed.
Draft genome sequence of biofilm-forming methicillin-resistant
Staphylococcus aureus
MTR_V1 strain isolated from a ready-to-eat food in Bangladesh
Authors: Ballah Fatimah Muhammad , Islam Md. Saiful , Ievy Samina , Ferdous Farhana Binte , Sobur Md. Abdus , Rahman AMM Taufiquer , Rahman Marzia , Hoque M. Nazmul , Hassan Jayedul , Rahman Md. Tanvir
Published: September 2023

This announcement provides the genome sequence of the biofilm-forming methicillin-resistant
Staphylococcus aureus
MTR_V1 strain isolated from a ready-to-eat food sample in Bangladesh. Our assembled genome had a length of 2.8 Mb, 27 contigs, two CRISPR arrays, 38 predicted antibiotic resistance genes, and 66 predicted virulence factor genes.
Successful delayed interval delivery of a triplet pregnancy using conservative management
Authors: Eckel Fanny , Klebermass-Schrehof Katrin , Bago-Horvath Zsuzsanna , Farr Alex
Published: September 2023

Preterm birth is a significant cause of perinatal morbidity and mortality, especially in multiple pregnancies. Delayed interval delivery can prolong pregnancy for the remaining fetus(es) in an imminent stillbirth or extremely preterm birth of the first fetus, improving the lastborn’s outcomes. We present a case of delayed interval delivery of a triplet pregnancy following preterm prelabour rupture of membranes and progressive cervical insufficiency. Following vaginal delivery of the first fetus at 24+1 gestational weeks, the patient received antibiotics and tocolysis. Cerclage was not conducted as the mother had a vaginal infection. A 15-day delivery interval for the second and third fetuses was achieved. The firstborn required mechanical ventilation and inotropic support, while the others only required continuous positive airway pressure. There is no consensus on the best way to perform delayed interval delivery. We achieved a complications-free interval of 15 days with conservative management in a triplet pregnancy.
Incidence of infections in patients with psoriatic arthritis and axial spondyloarthritis treated with biological or targeted disease-modifying agents: a systematic review and meta-analysis of randomised controlled trials, open-label studies and observational studies
Authors: Aureal Melanie , Seauve Milene , Laplane Soline , Lega Jean-Christophe , Cabrera Natalia , Coury Fabienne
Published: September 2023

Objective

To estimate the incidence of infections among patients with psoriatic arthritis (PsA) or axial spondyloarthritis (axSpA), two distinct phenotypes included in the large group of spondyloarthritis (SpA), treated with tumour necrosis-factor-inhibitors, interleukin-17-inhibitors, Janus kinase-inhibitors, IL-23 or IL-12/23-inhibitors (IL-12/23i), phosphodiesterase 4-inhibitors or cytotoxic T-lymphocyte associated protein 4-Ig.

Methods

A meta-analysis of randomised controlled trials (RCTs), open-label extension and observational studies was conducted. Serious infections were defined as infections that were life-threatening, required intravenous antibiotics and/or hospitalisation. Non-serious infections did not meet these severity criteria. The incidence rates (IR) were reported for each diagnosis by treatment class and study type using random-effect model to create a 95% CI.

Results

Among 23 333 PsA patients and 11 457 axSpA patients, there were 1.09 serious infections per 100 patient-years (PY) (95% CI 0.85 to 1.35) with similar IR in PsA (0.96 per 100 PY 95% CI 0.69 to 1.28) and axSpA (1.09 per 100 PY 95% CI 0.76 to 1.46). The IR was lower in RCTs (0.77 per 100 PY 95% CI 0.41 to 1.20) compared with observational studies (1.68 per 100 PY 95% CI 1.03 to 2.47). In PsA patients, the lowest IR value was observed with IL-12/23i (0.29 per 100 PY 95% CI 0.00 to 1.03). There were 53.0 non-serious infections per 100 PY (95% CI 43.47 to 63.55) in 7257 PsA patients and 5638 axSpA patients. The IR was higher in RCTs (69.95 per 100 PY 95% CI 61.59 to 78.84) compared with observational studies (15.37 per 100 PY 95% CI 5.11 to 30.97).

Conclusion

Serious infections were rare events in RCTs and real-life studies. Non-serious infections were common adverse events, mainly in RCTs.

PROSPERO registration number

CRD42020196711
Parents’ knowledge of the 2019 Canada’s Food Guide recommendations
Authors: Ramuscak Alyssa V. , Duncan Alison M. , Ma David W.L. , Haines Jess ,   on behalf of the Guelph Family Health Study
Published: September 2023

Little is known about Canadians’ knowledge of the 2019 Canada’s Food Guide (CFG) recommendations. Using cross-sectional survey data from the Guelph Family Health Study, our brief communication aimed to explore parents’ knowledge of the 2019 CFG recommendations through descriptive statistics and content analysis. Among a sample of 122 parents, we found that parents had a general understanding of the 2019 CFG’s recommendations but poor knowledge of specific details of the recommendations, such as the names of the three food groups and which foods contain unhealthy fats.
Evaluation of Vicia faba plant genotypes using non-destructive vibrational molecular spectroscopic technique (ATR-FT/IR): molecular structure profiles of protein in relation to nutritional characteristics
Authors: Espinosa Maria E. Rodríguez , Yu Peiqiang
Published: September 2023

This study aimed to evaluate the impact of genotypes and tannin levels on the nutritional characteristics of faba bean seeds and their relation to protein molecular structure profiles using Fourier transform infrared spectroscopy (FTIR). Eight genotypes with low tannin (LT, n = 3) and normal tannin (NT, n = 5) levels were studied. Standard methods of analysis and NRC 2001 were used to obtain chemical and energy parameters. The protein molecular spectral study was performed using a spectrometer JASCO FT/IR–4200. Molecular features were analyzed in the mid-infrared region at ~4000–800 cm−1 and protein-related functional groups were obtained using OMNIC software. Results showed higher ( P < 0.01) soluble crude protein (SCP), rapidly degradable protein fraction (PA2), and intestinal digestibility of bypass protein (dIDP) in LT than NT. The slowly degradable protein fraction (PB2) was higher in NT ( P < 0.01) compared to LT. Higher absorbance was observed in spectral peaks related to amide I, amide II, and β-sheet in NT ( P < 0.05) compared to LT. The area ratio of amide I to amide II and the height ratio of α-helix to β-sheet were higher in LT compared to NT ( P < 0.01). This study provides a better understanding of associations between structure and nutritional characteristics in faba bean genotypes with different tannin levels.
Minor minerals, major implications: using key mineral phases to unravel the formation and evolution of Earth's crust
Authors: van Schijndel Valby , Cutts Kathryn A. , Pereira Inês , Guitreau Martin , Volante Silvia , Tedeschi Mahyra
Published: September 2023

The investigation of key minerals including zircon, apatite, titanite, rutile, monazite, xenotime, allanite, baddeleyite and garnet can retain critical information about petrogenetic and geodynamic processes and may be utilized to understand complex geological histories and the dynamic evolution of the continental crust. They act as small but often robust petrochronological capsules and provide information about crustal evolution, from local processes to plate tectonics and supercontinent cycles. They offer us insights into processes of magmatism, sedimentation, metamorphism and alteration, even when the original protolith is not preserved.
In situ
techniques have enabled a more in-depth understanding of trace element behaviour in these minerals within their textural context. This has led to more meaningful ages for many stages of geological events. New developments of analytical procedures have further allowed us to expand our petrochronological toolbox while improving precision and accuracy. Combining multiple proxies with multiple minerals has contributed to new interpretations of the crustal history of our planet.
Taming Irregular Cardiac Signals for Biometric Identification
Authors: Wang Weizheng , Wang Qing , Zuniga Marco
Published: September 2023

Cardiac patterns are being used to provide hard-to-forge biometric signatures in identification applications. However, this performance is obtained undercontrolled scenarioswhere cardiac signals maintain a relatively uniform pattern, facilitating the identification process. In this work, we analyze cardiac signals collected in morerealistic (uncontrolled) scenariosand show that their high signal variability makes them harder to obtain stable and distinct features. When faced with these irregular signals, the state-of-the-art (SOTA) reduces its performance significantly. To solve these problems, we propose the CardioID framework1with two novel properties. First, we design an adaptive method that achieves stable and distinct features by tailoring the filtering process according to each user’s heart rate. Second, we show that users can have multiple cardiac morphologies, offering us a bigger pool of cardiac signals compared to the SOTA. Considering threeuncontrolleddatasets, our evaluation shows two main insights. First, while using a PPG sensor with healthy individuals, the SOTA’s balanced accuracy (BAC) reduces from 90–95% to 75–80%, while our method maintains a BAC above 90%. Second, under more challenging conditions (using smartphone cameras or monitoring unhealthy individuals), the SOTA’s BAC reduces to values between 65–75%, and our method increases the BAC to values between 75–85%.
Montgomery Multiplication Scalable Systolic Designs Optimized for DSP48E2
Authors: Noyez Louis , Mrabet Nadia El , Potin Olivier , Veron Pascal
Published: September 2023

This article describes an extensive study of the use of DSP48E2 Slices in Ultrascale FPGAs to design hardware versions of the Montgomery Multiplication algorithm for the hardware acceleration of modular multiplications. Our fully scalable systolic architectures result in parallelized, DSP48E2-optimized scheduling of operations analogous to the FIOS block variant of the Montgomery Multiplication. We explore the impacts of different pipelining strategies within DSP blocks, scheduling of operations, processing element configurations, global design structures and their tradeoffs in terms of performance and resource costs. We discuss the application of our methodology to multiple types of DSP primitives. We provide ready-to-use fast, efficient, and fully parametrizable designs, which can adapt to a wide range of requirements and applications. Implementations are scalable to any operand width. Our most efficient designs can perform 128, 256, 512, 1024, 2048, and 4096 bits Montgomery modular multiplications in 0.0992 μs, 0.2032 μs, 0.3952 μs, 0.7792μs, 1.550 μs, and 3.099 μs using 4, 6, 11, 21, 41, and 82 DSP blocks, respectively.
Electron Transfer Beyond the Outer Membrane: Putting Electrons to Rest
Authors: Gralnick J.A. , Bond D.R.
Published: September 2023

Extracellular electron transfer (EET) is the physiological process that enables the reduction or oxidation of molecules and minerals beyond the surface of a microbial cell. The first bacteria characterized with this capability were Shewanella and Geobacter, both reported to couple their growth to the reduction of iron or manganese oxide minerals located extracellularly. A key difference between EET and nearly every other respiratory activity on Earth is the need to transfer electrons beyond the cell membrane. The past decade has resolved how well-conserved strategies conduct electrons from the inner membrane to the outer surface. However, recent data suggest a much wider and less well understood collection of mechanisms enabling electron transfer to distant acceptors. This review reflects the current state of knowledge from Shewanella and Geobacter, specifically focusing on transfer across the outer membrane and beyond—an activity that enables reduction of highly variable minerals, electrodes, and even other organisms.
Raising a Bacterium to the Rank of a Model System: The Listeria Paradigm
Authors: Cossart Pascale
Published: September 2023

My scientific career has resulted from key decisions and reorientations, sometimes taken rapidly but not always, guided by discussions or collaborations with amazing individuals from whom I learnt a lot scientifically and humanly. I had never anticipated that I would accomplish so much in what appeared as terra incognita when I started to interrogate the mechanisms underlying the virulence of the bacterium Listeria monocytogenes. All this has been possible thanks to a number of talented team members who ultimately became friends.
The Relation Between Attention and Memory
Authors: Cowan Nelson , Bao Chenye , Bishop-Chrzanowski Brittney M. , Costa Amy N. , Greene Nathaniel R. , Guitard Dominic , Li Chenyuan , Musich Madison L. , Ünal Zehra E.
Published: September 2023

The relation between attention and memory has long been deemed important for understanding cognition, and it was heavily researched even in the first experimental psychology laboratory by Wilhelm Wundt and his colleagues. Since then, the importance of the relation between attention and memory has been explored in myriad subdisciplines of psychology, and we incorporate a wide range of these diverse fields. Here, we examine some of the practical consequences of this relation and summarize work with various methodologies relating attention to memory in the fields of working memory, long-term memory, individual differences, life-span development, typical brain function, and neuropsychological conditions. We point out strengths and unanswered questions for our own embedded processes view of information processing, which is used to organize a large body of evidence. Last, we briefly consider the relation of the evidence to a range of other theoretical views before drawing conclusions about the state of the field.
A Flexible Self-Supported Silver Wire with Nanoporous Skeleton for Electrochemical Sensing of Trichloroacetic Acid
Authors: Zhao Fengming , Zhao Lili , Wang Xinhang , Hu Huadong , Zhu Yinghong , Chu Youqun , Chen Wanli
Published: September 2023

The content of chloroacetic acid in water is mandatory, so rapid and accurate detection is of great significance for environmental health. Silver has a strong specific interaction with chloridion and its three-dimensional interconnected ligaments are proved to have higher activity. Herein, a self-supported nanoporous skeleton was constructed on the surface of silver wire by directly electrochemical alloying in ZnCl2–glycol solution at 140 °C and subsequently dealloying in sulfuric acid solution. AgZn and AgZn3 alloys are formed by electrodeposition process, and the nanoporous skeleton of elemental silver is formed after dealloying. The interconnected ligaments are uniform and the pore size is about 10–200 nm. The cyclic voltammetry showed that the self-supported nanoporous skeleton of the silver wire electrode exhibited high electrocatalytic activity for trichloroacetic acid. Detected by chronoamperometry, the results show that the detection limit is 70 nM (S/N = 3) in the concentration range of 0.14–549 μM, and the sensor has high sensitivity, good reproducibility and anti-interference. And by detecting the recovery rate of trichloroacetic acid in the tap water, river water and industrial wastewater, the sensor proves the practicality of the prepared electrode.
Mechanistic insights on age-related changes in heart-aorta-brain hemodynamic coupling using a pulse wave model of the entire circulatory system
Authors: Aghilinejad Arian , Amlani Faisal , Mazandarani Sohrab P. , King Kevin S. , Pahlevan Niema M.
Published: September 2023

We investigated the impact of major aging mechanisms of the arterial system and cardiac function on brain hemodynamics. Our findings suggest that aging has a significant impact on heart-aorta-brain coupling through changes in both arterial stiffening and left ventricle (LV) contractility. Understanding the underlying physical mechanisms involved here can potentially be a key step for developing more effective therapeutic strategies that can mitigate the contributions of abnormal LV-arterial coupling toward neurodegenerative diseases and dementia.
Influence of Deposition Parameters of ITO Films on the Performance of HJT Solar Cells
Authors: Huang Guoping , Zhao Guixiang , Huang Xixi , Zhuang Hao , Zhang Zhongjian , Gao Ronggang , Xie Fengxian
Published: September 2023

TCO (transparent conductive oxide) films are widely used in solar cells due to the characteristics of transparency and conductivity. In this paper, ITO (indium tin oxide) transparent conductive films are prepared on common slides by DC magnetron sputtering, and the preparation process and characteristics of ITO films are studied. The target for sputtering is ITO, with the mass ratio of In203 and Sn02 was 90% : 10%. The sheet resistance, carrier concentration, and carrier mobility of ITO films are measured and analyzed by a UV-Vis spectrophotometer, four-point probe, and Hall effect measurement system. By changing the oxygen content, deposition temperature, and sputtering power to studied the effects on the light transmittance and electrical conductivity of the ITO films, further studied the effects on the HJT (heterojunction with intrinsic thin film) solar cells, and finally determined the appropriate preparation parameters. Results show that the resistance is

6
.

4

∗

10

−
4

 
Ω
•
cm

, the light transmittance is beyond 90.6%, efficiency is 23.78%, and bifacial ratio is 84% when oxygen content is 2.2%, sputtering power is 3 kw, and deposition temperature is 190°C.
Development and Experimental Study of a Self-Aware Posttensioned Prestressed Intelligent Reinforcement Structure
Authors: Li Guodong , Jing Xiaotong , Wang Jinbo , Lan Chunguang
Published: September 2023

A self-aware posttensioned prestressing intelligent reinforcement system with force and sensors is proposed by combining the advantages of posttensioned prestressing reinforcement technology and intelligent structure. A static test study is then conducted with brick masonry members (wall pieces) as reinforcement objects. The experimental results demonstrate that the intelligent strand can be deployed using the same process, tensioning apparatus, and anchoring equipment as ordinary strand. Furthermore, the mechanical performance indexes with and without intelligent strands are very close, and the differences in percentages of cracking load, cracking displacement, yielding load, yielding displacement, ultimate load, and ultimate displacement are 20%, 9%, 9%, 10%, 2%, and 26%.
Levetiracetam-Induced Acute Psychosis in an Adolescent
Authors: Dhungel Omkar , Shrestha Amit , Pathak Pankaj , Sharma Pawan
Published: September 2023

Levetiracetam (LEV), a second-generation antiepileptic, is used as an adjunct therapy in primary generalized tonic–clonic seizures, refractory partial-onset seizures, and seizure prophylaxis after brain surgery. It is well tolerated, effective and has a convenient dosing regimen. As any other drugs, it has some adverse drug effects, including neuropsychiatric adverse effects ranging from agitation and mood symptoms to psychosis and suicide. Strong diagnostics guidelines are yet to be formulated for LEV-induced psychosis; however, complete recovery from psychotic symptoms after stopping LEV supports the possible adverse reaction from Naranjo’s algorithm and, hence, the diagnosis. This case report presents a 16 years boy with focal onset generalized tonic–clonic seizure, whose drug regimen was switched to LEV, following which he had the delusion of persecution, second-person auditory hallucination, and aggressive behavior, which decreased on the 2nd day of cessation of LEV.
Analysis and Detection of Road Traffic Accident Severity via Data Mining Techniques: Case Study Addis Ababa, Ethiopia
Authors: Endalie Demeke , Abebe Wondmagegn Taye
Published: September 2023

Around the world, road traffic accidents are the leading cause of serious injuries and deaths. Ethiopia is one of the countries that suffer the most from traffic accidents. Every government in every country wants to keep its citizens safe from accidents. To keep people safe from accidents, it is necessary to conduct a detailed analysis of the factors that contribute to high‐severity accidents and deaths. As a result, we developed a data mining algorithm‐based road traffic accident severity analysis for the Addis Ababa subcity in this study. The longest frequent factors in the dataset were generated using the Apriori algorithm. The Apriori algorithm generates the most frequent factors as sex: male, driver–vehicle relationship: employee, weather condition: normal, pedestrian movement: not a pedestrian, road surface type: asphalt, and accident severity: high severity, with 42.21% and 84.35% support and confidence, respectively. In addition, we created an accident severity level predictive model using a support vector machine. The predictive model has an accuracy of 85%. The proposed predictive model outperforms other well‐known predictive models, such as K‐nearest neighbors, decision trees, and random forests. As a result, when making decisions or policies in Ethiopia, the government or private organizations should consider the association of factors that lead to serious severe accidents.
Revisiting Gendered ICT Attitude and Self-Efficacy: A Study of Technical University Students in Ghana
Authors: Mensah Christopher , Azila-Gbettor Edem M. , Kugbonu Mawufemor A. , Tahiru Fati
Published: September 2023

The existence of gender differences in the use of information and communication technology (ICT) impedes the attainment of gender equality and female empowerment. It is therefore essential to provide up-to-date knowledge on the gender ICT divide given that insight into ICT use by gender is often limited and not regularly updated, particularly for low- and lower-middle-income countries. Consequently, this study investigated gender differences in ICT usage, self-efficacy, attitude, and anxiety among technical university students in Ghana. The study is based on primary data conveniently collected from 409 students in a 3-year Higher National Diploma awarding technical university in Ghana using a self-administered questionnaire. Data were analyzed using descriptive statistics, Mann–Whitney U tests, and ordered logistic regression. Male students, compared to their female peers, reported frequent use of ICT, have a more confident and positive attitude, and are less anxious about the use of computers and their related applications. Access to computers and students’ level of study were related to attitude and perceived ICT self-efficacy. Policymakers and administrators of higher education institutions should implement interventions to improve female self-confidence and the use of computers and their applications.
Optimal Deployment for Hybrid Sensor Networks Based on Efficient Node Configuration
Authors: Sun Qian , Peng Xiao , Wang Xiaoyi , Zhao Zhiyao , Xu Jiping , Wang Li , Zhang Huiyan , Yu Jiabin , Bai Yuting
Published: September 2023

Hybrid sensor networks, which contain mobile nodes and stationary nodes, are being used more and more widely. The second deployment of mobile nodes is a key problem to be solved, and the deployment performance of the network directly affects the monitoring effect of the network. Optimizing the configuration ratio of the two nodes can effectively reduce the network cost. In this paper, under the premise of knowing the coverage of the required monitoring area, the impact of sensor devices on node configuration is studied through parameter analysis, and the number and types of sensors that should be deployed in the hybrid sensor network are deduced, which can be conveniently and accurately used to design the actual hybrid sensor network. At the same time, for the secondary deployment of mobile nodes, this paper proposes a new mobile coverage method BS‐CCP (box search and concentric circle positioning) to improve the coverage of the hybrid sensor network and maximize the coverage of the target area with the specified sensor types and numbers. Compared with existing work, the method in this paper reduces the number of iterations and reduces the number of required nodes. Comparing BS‐CCP with the existing network mobile coverage algorithm, the experimental results show that the coverage obtained by this method is larger and more efficient.
Adolescent- and Young Adult-Reported Outcomes and Use of Continuous Glucose Monitoring Features: A Report from the CITY Trial
Authors: Messer Laurel H. , Bauza Colleen , Miller Kellee M. , Clements Mark A. , DeSalvo Daniel J. , Sherr Jennifer , Weinstock Ruth S. , Hood Korey , Laffel Lori M.
Published: September 2023

Objective. To evaluate patterns of continuous glucose monitor (CGM) use and perceptions of quality of life in adolescents/young adults with type 1 diabetes (T1D) after using CGM for up to 52 weeks in the CGM Intervention in Teens and Young (CITY) Adults randomized clinical trial (RCT). Subjects and Methods. Participants with T1D were initially randomized 1 : 1 to use of CGM or blood glucose meter (BGM) for 26 weeks. Following the RCT, participants in the BGM group initiated CGM (BGM–CGM cohort) and participants in the CGM group continued CGM (CGM–CGM cohort) for another 26 weeks. Problem Areas in Diabetes Survey-Pediatric Version (PAID-peds), Glucose Monitoring Satisfaction Survey (GMSS), Hypoglycemia Confidence Scale (HCS), Diabetes Technology Attitudes (DTA), Pittsburgh Sleep Quality Index (PSQI), Benefits of CGM, and Burdens of CGM were completed at baseline, 26 and 52 weeks. Results. In both cohorts, >70% of participants were wearing CGM > 5 days/week at 52 weeks; 5% discontinued CGM. The majority used the mobile app to receive glucose data. Adolescents (14 to <19 years) were more likely to use SHARE features than young adults (80% versus 41%). CGM–CGM participants had significantly higher scores on GMSS, DTA, and HCS at 52 weeks compared with baseline, and reported higher benefit and lower burden perceptions than at baseline. Similar results were observed for the BGM–CGM cohort. Conclusions. Improvements in self-reported measures were observed in adolescents and young adults using CGM. As CGM use is also associated with better glycemic control, utilizing CGM may contribute to improving both medical outcomes and emotional health.
Prediction of Esophageal Varices in Viral Hepatitis C Cirrhosis: Performance of Combined Ultrasonography and Clinical Predictors
Authors: Charoenchue Puwitch , Na Chiangmai Wittanee , Amantakul Amonlaya , Wanchaitanawong Wasuwit , Chitapanarux Taned , Pojchamarnwiputh Suwalee
Published: September 2023

Objectives. This study is aimed at evaluating the diagnostic performance of clinical predictors and the Doppler ultrasonography in predicting esophageal varices (EV) in patients with hepatitis C-related cirrhosis and exploring the practical predictors of EV. Methods. We conducted a prospective study from July 2020 to January 2021, enrolling 65 patients with mild hepatitis C-related cirrhosis. We obtained clinical data and performed grayscale and the Doppler ultrasound to explore the predictors of EV. Esophagogastroduodenoscopy (EGD) was performed as the reference test by the gastroenterologist within a week. Results. The prevalence of EV in the study was 41.5%. Multivariable regression analysis revealed that gender (female,

OR
=
4.04

,

p
=
0.02

), platelet count (<150000 per ml,

OR
=
3.13

,

p
=
0.09

), splenic length (>11 cm,

OR
=
3.64

,

p
=
0.02

), and absent right hepatic vein (RHV) triphasicity (

OR
=
3.15

,

p
=
0.03

) were significant predictors of EV. However, the diagnostic accuracy indices for isolated predictors were not good (

AUROC
=
0.63

–0.66). A combination of these four predictors increases the diagnostic accuracy in predicting the presence of EV (

AUROC
=
0.80

, 95% CI 0.69-0.91). Furthermore, the Doppler assessment of the right hepatic vein waveform showed good reproducibility (

κ
=
0.76

). Conclusion. Combining clinical and Doppler ultrasound features can be used as a screening test for predicting the presence of EV in patients with hepatitis C-related cirrhosis. The practical predictors identified in this study could serve as an alternative to invasive EGD in EV diagnosis. Further studies are needed to explore the diagnostic accuracy of additional noninvasive predictors, such as elastography, to improve EV screening.
Intelligent Integrated Approach for Voltage Balancing Using Particle Swarm Optimization and Predictive Models
Authors: Ghaeb Jasim , Al-Naimi Ibrahim , Alkayyali Malek
Published: September 2023

In this paper, an intelligent integrated approach is proposed to control the reactive power and restore the voltage balance in a three-phase power system using particle swarm optimization (PSO), Gaussian process regression (GPR), and support vector machine (SVM). The PSO algorithm is used in offline mode to determine the optimal set of firing angles for the thyristor-controlled-reactor (TCR) compensator according to the smallest fitness value required for voltage balancing. The optimum firing angles are then used to train the GPR and SVM regression models. The GPR and SVM models are finally used as a real-time controller to retrieve the voltage balance in online mode. A simulation model and experimental setup of the electrical power system are built. The modeled system consists of a 500 km long transmission line. The line is divided into three-pi sections to guarantee a real system response. Several simulation and practical case studies have been conducted to test and validate the capability of the proposed integrated approach in solving the voltage unbalance problem. The results have revealed the supreme ability of the proposed integrated approach to restore the voltage balance quickly (within 20 ms) and for a wide range of voltage unbalance factors (VUFs) (3.90–8.42%).
Supplementary Data from Mutant KRAS Mediates circARFGEF2 Biogenesis to Promote Lymphatic Metastasis of Pancreatic Ductal Adenocarcinoma
Authors: Kong Yao , Luo Yuming , Zheng Shangyou , Yang Jiabin , Zhang Dingwen , Zhao Yue , Zheng Hanhao , An Mingjie , Lin Yan , Ai Le , Diao Xiayao , Lin Qing , Chen Changhao , Chen Rufu
Published: September 2023

Figure S1-S10, Table S1-S5 and Supplementary methods
Table S5 from CD25high Effector Regulatory T Cells Hamper Responses to PD-1 Blockade in Triple-Negative Breast Cancer
Authors: Fattori Stéphane , Le Roy Aude , Houacine Jemila , Robert Lucie , Abes Riad , Gorvel Laurent , Granjeaud Samuel , Rouvière Marie-Sarah , Ben Amara Amira , Boucherit Nicolas , Tarpin Carole , Pakradouni Jihane , Charafe-Jauffret Emmanuelle , Houvenaeghel Gilles , Lambaudie Eric , Bertucci François , Rochigneux Philippe , Gonçalves Anthony , Foussat Arnaud , Chrétien Anne-Sophie , Olive Daniel
Published: September 2023

Differentially expressed genes between intratumoral TNFRSF9+ eTregs versus TNFRSF9- eTregs, related to Figure 1
Data from Apolipoprotein E2 Stimulates Protein Synthesis and Promotes Melanoma Progression
Authors: Adaku Nneoma , Ostendorf Benjamin N. , Mei Wenbin , Tavazoie Sohail F.
Published: September 2023

Abstract
The secreted lipid transporter apolipoprotein E (APOE) plays important roles in atherosclerosis and Alzheimer’s disease and has been implicated as a suppressor of melanoma progression. The APOE germline genotype predicts human melanoma outcomes, with APOE4 and APOE2 allele carriers exhibiting prolonged and reduced survival, respectively, relative to APOE3 homozygotes. While the APOE4 variant was recently shown to suppress melanoma progression by enhancing anti-tumor immunity, further work is needed to fully characterize the melanoma cell-intrinsic effects of APOE variants on cancer progression. Using a genetically engineered mouse model, we showed that human germline APOE genetic variants differentially modulate melanoma growth and metastasis in an APOE2>APOE3>APOE4 manner. The LRP1 receptor mediated the cell-intrinsic effects of APOE variants on melanoma progression. Protein synthesis was a tumor cell-intrinsic process differentially modulated by APOE variants, with APOE2 promoting translation via LRP1. These findings reveal a gain-of-function role for the APOE2 variant in melanoma progression, which may aid in predicting melanoma patient outcomes and understanding the protective effect of APOE2 in Alzheimer’s disease.
Data from Dual inhibition of KRASG12D and pan-ERBB is synergistic in pancreatic ductal adenocarcinoma
Authors: Gulay Kevin Christian Montecillo. , Zhang Xinlian , Pantazopoulou Vasiliki , Patel Jay , Esparza Edgar , Pran Babu Deepa Sheik. , Ogawa Satoshi , Weitz Jonathan , Ng Isabella , Mose Evangeline S. , Pu Minya , Engle Dannielle D. , Lowy Andrew M. , Tiriac Hervé
Published: September 2023

Abstract
Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with a low survival rate. Recently, new drugs that target KRASG12D, a common mutation in PDAC, have been developed. We studied one of these compounds, MRTX1133, and found it was specific and effective at low nanomolar concentrations in patient-derived organoid models and cell lines harboring KRASG12D mutations. Treatment with MRTX1133 upregulated the expression and phosphorylation of EGFR and HER2, indicating that inhibition of ERBB signaling may potentiate MRTX1133 anti-tumor activity. Indeed, the irreversible pan-ERBB inhibitor, afatinib, potently synergized with MRTX1133 in vitro, and cancer cells with acquired resistance to MRTX1133 in vitro remained sensitive to this combination therapy. Finally, the combination of MRTX1133 and afatinib led to tumor regression and longer survival in orthotopic PDAC mouse models. These results suggest that dual inhibition of ERBB and KRAS signaling may be synergistic and circumvent the rapid development of acquired resistance in patients with KRAS mutant pancreatic cancer.
Data from CD25high Effector Regulatory T Cells Hamper Responses to PD-1 Blockade in Triple-Negative Breast Cancer
Authors: Fattori Stéphane , Le Roy Aude , Houacine Jemila , Robert Lucie , Abes Riad , Gorvel Laurent , Granjeaud Samuel , Rouvière Marie-Sarah , Ben Amara Amira , Boucherit Nicolas , Tarpin Carole , Pakradouni Jihane , Charafe-Jauffret Emmanuelle , Houvenaeghel Gilles , Lambaudie Eric , Bertucci Francois , Rochigneux Philippe , Gonçalves Anthony , Foussat Arnaud , Chrétien Anne-Sophie , Olive Daniel
Published: September 2023

Abstract
Regulatory T cells (Tregs) impede effective antitumor immunity. However, the role of Tregs in clinical outcomes of patients with triple-negative breast cancer (TNBC) remains controversial. Here, we found that an immunosuppressive TNBC microenvironment is marked by an imbalance between effector αβCD8+ T cells and Tregs harboring hallmarks of highly suppressive effector Tregs (eTregs). Intratumoral eTregs strongly expressed PD-1 and persisted in patients with TNBC resistant to PD-1 blockade. Importantly, CD25 was the most selective surface marker of eTregs in primary TNBC and metastases compared with other candidate targets for eTreg depletion currently being evaluated in trials for patients with advanced TNBC. In a syngeneic TNBC model, the use of Fc-optimized, IL-2 sparing, anti-CD25 antibodies synergized with PD-1 blockade to promote systemic antitumor immunity and durable tumor growth control by increasing effector αβCD8+ T cell/Treg ratios in tumors and in the periphery. Together, this study provides the rationale for clinical translation of anti-CD25 therapy to improve PD-1 blockade responses in patients with TNBC.
Supplementary Figure S10 from Determinants of Survival with Combined HER2 and PD-1 Blockade in Metastatic Esophagogastric Cancer
Authors: Maron Steven B. , Chatila Walid , Walch Henry , Chou Joanne F. , Ceglia Nicholas , Ptashkin Ryan , Do Richard Kinh Gian , Paroder Viktoriya , Pandit-Taskar Neeta , Lewis Jason S. , Biachi De Castria Tiago , Sabwa Shalom , Socolow Fiona , Feder Lara , Thomas Jasmine , Schulze Isabell , Kim Kwanghee , Elzein Arijh , Bojilova Viktoria , Zatzman Matthew , Bhanot Umesh , Nagy Rebecca J. , Lee Jeeyun , Simmons Marc , Segal Michal , Ku Geoffrey Yuyat , Ilson David H. , Capanu Marinela , Hechtman Jaclyn F. , Merghoub Taha , Shah Sohrab , Schultz Nikolaus , Solit David B. , Janjigian Yelena Y.
Published: September 2023

Identifying intratumor clonal selection and associated resistance mechanisms. a, Inferred tumor clones paired pre-treatment and post-cycle 2 CAPOX + trastuzumab scRNA-seq demonstrates rapid clearance of highly ERBB2 expression (red) clones, while many lower expressing clones persisted and even expanded. Contraction at the on-treatment timepoint reflects their RECIST response at their initial follow up imaging timepoint, demonstrating that patient 3, who had the highest ERBB2 expression, had the deepest and most durable response. b, Pre- and on-treatment size and fold-change of clones identified in pane a ordered by pre-treatment ERBB2 expression. Clonal expansion was most commonly seen in clones with low ERBB2 expression and was associated with a resistance program derived by GeneVector. c, MT1H, MT1E, MT2A, and MSMB expression were significantly higher in clones expanding despite CAPOX + trastuzumab (TC) versus both contracting clones after TC as well as expanding clones after CAPOX (C) only, suggesting association with therapeutic resistance. d, The resistance program strongly correlated with the CAPOX+trastuzumab clones, and poorly with CAPOX-treated clones, as intended.
Figure S4 from A Novel HOXA10-Associated 5-Gene–Based Prognostic Signature for Stratification of Short-term Survivors of Pancreatic Ductal Adenocarcinoma
Authors: Kisling Sophia G. , Atri Pranita , Shah Ashu , Cox Jesse L. , Sharma Sunandini , Smith Lynette M. , Ghersi Dario , Batra Surinder K.
Published: September 2023

CIBERSORT analysis for T cell exhaustion markers
Supplementary Figure S3 from Estrogen Therapy Induces Receptor-Dependent DNA Damage Enhanced by PARP Inhibition in ER+ Breast Cancer
Authors: Traphagen Nicole A. , Schwartz Gary N. , Tau Steven , Roberts Alyssa M. , Jiang Amanda , Hosford Sarah R. , Marotti Jonathan D. , Goen Abigail E. , Romo Bianca A. , Johnson Anneka L. , Duffy Emily-Claire K. , Demidenko Eugene , Heverly Paul , Mosesson Yaron , Soucy Shannon M. , Kolling Fred , Miller Todd W.
Published: September 2023

Supplementary Figure S3- E2-induced DNA damage is dependent upon cell cycle progression.
Supplementary Figure S14 from Estrogen Therapy Induces Receptor-Dependent DNA Damage Enhanced by PARP Inhibition in ER+ Breast Cancer
Authors: Traphagen Nicole A. , Schwartz Gary N. , Tau Steven , Roberts Alyssa M. , Jiang Amanda , Hosford Sarah R. , Marotti Jonathan D. , Goen Abigail E. , Romo Bianca A. , Johnson Anneka L. , Duffy Emily-Claire K. , Demidenko Eugene , Heverly Paul , Mosesson Yaron , Soucy Shannon M. , Kolling Fred , Miller Todd W.
Published: September 2023

Supplementary Figure S14- Individual growth curves of tumors from mice treated ± E2 and/or olaparib.
Supplementary Figure S13 from Estrogen Therapy Induces Receptor-Dependent DNA Damage Enhanced by PARP Inhibition in ER+ Breast Cancer
Authors: Traphagen Nicole A. , Schwartz Gary N. , Tau Steven , Roberts Alyssa M. , Jiang Amanda , Hosford Sarah R. , Marotti Jonathan D. , Goen Abigail E. , Romo Bianca A. , Johnson Anneka L. , Duffy Emily-Claire K. , Demidenko Eugene , Heverly Paul , Mosesson Yaron , Soucy Shannon M. , Kolling Fred , Miller Todd W.
Published: September 2023

Supplementary Figure S13- E2 and olaparib combine to increase R-loop formation.
Supplemental Figure 6 from Transcriptomic Signatures of MSI-High Metastatic Colorectal Cancer Predict Efficacy of Immune Checkpoint Inhibitors
Authors: Gallois Claire , Landi Matteo , Taieb Julien , Sroussi Marine , Saberzadeh-Ardestani Bahar , Cazelles Antoine , Lonardi Sara , Bergamo Francesca , Intini Rossana , Maddalena Giulia , Pietrantonio Filippo , Corti Francesca , Ambrosini Margherita , Martinetti Antonia , Germani Marco Maria , Boccaccio Chiara , Vetere Guglielmo , Mouillet-Richard Sophie , de Reynies Aurélien , Sinicrope Frank A. , Cremolini Chiara , Laurent-Puig Pierre
Published: September 2023

Survival after recurrence in MSI patients from PETACC8 trial with available 3’RNAseq data
Supplemental Figure 4 from Transcriptomic Signatures of MSI-High Metastatic Colorectal Cancer Predict Efficacy of Immune Checkpoint Inhibitors
Authors: Gallois Claire , Landi Matteo , Taieb Julien , Sroussi Marine , Saberzadeh-Ardestani Bahar , Cazelles Antoine , Lonardi Sara , Bergamo Francesca , Intini Rossana , Maddalena Giulia , Pietrantonio Filippo , Corti Francesca , Ambrosini Margherita , Martinetti Antonia , Germani Marco Maria , Boccaccio Chiara , Vetere Guglielmo , Mouillet-Richard Sophie , de Reynies Aurélien , Sinicrope Frank A. , Cremolini Chiara , Laurent-Puig Pierre
Published: September 2023

Heatmap with unsupervised clustering in the validation cohort
Supplementary Figure S1 from A Phase I, Multicenter, Open-Label, First-in-Human Study of DS-6157a in Patients with Advanced Gastrointestinal Stromal Tumor
Authors: George Suzanne , Heinrich Michael C. , Somaiah Neeta , Oppelt Peter , McLeod Robert , Nishioka Satoshi , Kundu Madan G. , Qian Xiaozhong , Kumar Prasanna , Laadem Abderrahmane , Lau Yvonne , Tran Brittany P. , Fallon Maura , Dosunmu Ololade , Shi Julia , Naito Yoichi
Published: September 2023

Supplementary Figure S1. Swimmer plot of tumor response over time
Supplementary Table S3 from Trackable Intratumor Microdosing and Spatial Profiling Provide Early Insights into Activity of Investigational Agents in the Intact Tumor Microenvironment
Authors: Derry Jonathan M.J. , Burns Connor , Frazier Jason P. , Beirne Emily , Grenley Marc , DuFort Christopher C. , Killingbeck Emily , Leon Michael , Williams Claire , Gregory Mark , Houlton Jeffrey , Clayburgh Daniel , Swiecicki Paul , Huszar Dennis , Berger Allison , Klinghoffer Richard A.
Published: September 2023

Secondary Antibody Information
Supplementary Figure S9 from Trackable Intratumor Microdosing and Spatial Profiling Provide Early Insights into Activity of Investigational Agents in the Intact Tumor Microenvironment
Authors: Derry Jonathan M.J. , Burns Connor , Frazier Jason P. , Beirne Emily , Grenley Marc , DuFort Christopher C. , Killingbeck Emily , Leon Michael , Williams Claire , Gregory Mark , Houlton Jeffrey , Clayburgh Daniel , Swiecicki Paul , Huszar Dennis , Berger Allison , Klinghoffer Richard A.
Published: September 2023

Spatial Reconstruction of Patient 7 and Patient 8
Supplementary Methods S1 from CD49d Expression Identifies a Biologically Distinct Subtype of Chronic Lymphocytic Leukemia with Inferior Progression-Free Survival on BTK Inhibitor Therapy
Authors: Alsadhan Anfal , Chen Jonathan , Gaglione Erika M. , Underbayev Chingiz , Tuma Pamela L. , Tian Xin , Freeman Lita A. , Baskar Sivasubramanian , Nierman Pia , Soto Susan , Itsara Andy , Ahn Inhye E. , Sun Clare , Bibikova Elena , Hartmann Tanja Nicole , Mhibik Maissa , Wiestner Adrian
Published: September 2023

Supplementary Methods
Supplementary Figure S3 from CD49d Expression Identifies a Biologically Distinct Subtype of Chronic Lymphocytic Leukemia with Inferior Progression-Free Survival on BTK Inhibitor Therapy
Authors: Alsadhan Anfal , Chen Jonathan , Gaglione Erika M. , Underbayev Chingiz , Tuma Pamela L. , Tian Xin , Freeman Lita A. , Baskar Sivasubramanian , Nierman Pia , Soto Susan , Itsara Andy , Ahn Inhye E. , Sun Clare , Bibikova Elena , Hartmann Tanja Nicole , Mhibik Maissa , Wiestner Adrian
Published: September 2023

Supplementary Figure S3. In vivo effect of acalabrutinib on the VLA-4 activation upon BCR and chemokine signaling