bluuebunny/crossref_metadata_2025 huggingface dataset:
Academic Self-Realization of Researchers in Higher Education: Phenomenological Research-Based Evidence
Authors: Daugela Marius , Zydziunaite Vilma
Published: July 2024

In the dynamic context of higher education, the academic self-realization of a researcher is significant not only for the growth of the dedicated researcher but also for the well-being of the entire academic community. The purpose of this study was to reveal the academic self-realization experience of researchers in higher education and answer the research question: “What does academic self-realization mean for the researcher in higher education?” This study used a phenomenological-hermeneutic approach, which allowed researchers to delve into the academic self-realization experience of researchers in higher education. The study was conducted from November 2022 to December 2023 and represents the social sciences and the science of education. Individuals who have obtained a PhD, work as lecturers and/or researchers in Lithuanian universities, participate in the training of doctoral students, and who obtained bachelor’s, master’s, or doctoral degrees in more than one field of science took part in this study. This means that these researchers represent scientific multidisciplinarity. The research participants were from Lithuania and the United Kingdom. A total sample of seventeen researchers participated in the study. This article presents results from semi-structured interviews with six researchers, where the theme of “academic self-realization” emerged. The study results showed that the academic self-realization of the researcher in higher education is a process through which the researcher finds, develops, and represents personal interests and talents in the university’s academic community, thereby contributing to the personal, scientific, and academic development of this community. The conducted research reveals the complexity of the academic self-realization of researchers, which includes a significant integration of academic and personal components.
Quality of Experience That Matters in Gaming Graphics: How to Blend Image Processing and Virtual Reality
Authors: Jumani Awais Khan , Shi Jinglun , Laghari Asif Ali , Estrela Vania V. , Sampedro Gabriel Avelino , Almadhor Ahmad , Kryvinska Natalia , ul Nabi Aftab
Published: July 2024

This paper investigates virtual reality (VR) technology which can increase the quality of experience (QoE) on the graphics quality within the gaming environment. The graphics quality affects the VR environment and user experience. To gather relevant data, we conduct a live user experience and compare games with high- and low-quality graphics. The qualitative feedback obtained through questionnaires prove the importance ofcontextualizing users’ experiences playing both games. Furthermore, our findings prove the crucial role of graphics quality in adopting user engagement and enjoyment during gaming sessions. Users consistently reported their feeling more connected when interacting with games and receiving high-quality graphics. If the graphics quality received is low, the user rating for a particular game is low. Further examination of VR technology reveals its potential to revolutionize graphics quality within game play.
Software-Defined Wide Area Networks (SD-WANs): A Survey
Authors: Fu Chunle , Wang Bailing , Wang Wei
Published: July 2024

SD-WANs are an innovative software-defined network (SDN) technology used to reinvent networks, services, and applications in wide area network (WANs). The development of SD-WANs ranges from network optimization in the past to service provision platforms at present and distributed computing systems in the future. The existing surveys on SD-WANs are fragmented, covering specific problems only, and are not comprehensive with detailed research directions. This paper seeks to provide a systematic survey on SD-WANs by introducing major research directions and stating specific problems. Therefore, four major research directions related to traffic engineering, network optimization and systems, service orchestration, and the security issues of SD-WANs are sequentially introduced, along with detailed statements relating to specific problems and the classification of state-of-the-art research. Finally, the trends and challenges regarding SD-WANs are summarized and our future work is described.
Optimizing the Size of a Moving Annular Hollow Laser Heat Source
Authors: Ding Yongqi , Zhang Xiaohui
Published: July 2024

The physical phenomenon of the annular hollow laser surface treatment process is complex, and the internal mechanism involves multiple disciplines and fields. In addition to the general parameters of laser beams, such as laser power and scanning speed, an annular hollow laser beam exhibits unique physical characteristics, including hollow ratio and hollow area. The selection of the inner and outer annular radii of the laser plays a critical role in the study of metal surface heat treatment. From the point of view of heat transfer, the entransy dissipation theory is introduced in the metal surface treatment process with an annular hollow heat source. Firstly, using the principle of the extreme value of the entransy dissipation rate, under a constant heat flux boundary condition, the entransy dissipation rate is obtained through the temperature field distribution in the calculation area by numerical simulation. Secondly, the selection of the inner and outer ring radii of the annular laser is explored, and the average temperature difference of the heating surface is minimized to reduce the thermal stresses of the material. This paper seeks new insights into the geometric parameters of the inner and outer radii of the annular heat source.
Analysis of the Relationships between Variables and Their Applications in the Energy Saving Field
Authors: Zhu Yongqiang , Li Xinyi , Mu Xizhen , Zhao Yue
Published: July 2024

Energy saving is an important measure to promote social green transformation. The traditional energy-saving ideas usually only focus on a specific loss, and seldom consider the possible relationship and influence among various losses. In relatively complex energy-using systems, there are often many kinds of losses, and each loss may have many influencing factors. There may be some relationship between these losses and the influencing factors. To solve this problem, this paper presents an analysis method of the variable association in multi-variable systems. First, the basic relationships between variables and the representation methods are discussed. The basic concept of a path between variables is given, and the analysis method of variable association based on path statistics is provided. This paper focuses on the analysis of the influencing factors and paths of the observed variables, as well as which observed variables will be affected by a control variable. Then, based on the correlation matrix, the quantitative analysis method of the influence between variables is given. Variable correlation analysis is innovatively applied in the field of energy saving to determine the correlation of losses through variable associations, guiding the preliminary screening of energy-saving measures and analyzing the collateral effects of these measures. Based on the correlations between energy losses, a scientific process for formulating energy-saving measures is proposed. The variable correlation analysis method proposed in this paper is a generalized method, which can judge the correlation between variables from the perspective of theoretical analysis and avoid the dependence on data. In addition to good applications in the field of energy conservation, it can also be widely used in construction, transportation, climate change, and other fields. The proposed energy-saving ideas take into account the intensity of influencing factors on loss and the correlation between loss, which improves the effectiveness of energy saving measures.
Strategic Siting of Direct Air Capture Facilities in the United States
Authors: Boerst Jason , Pena Cabra Ivonne , Sharma Smriti , Zaremsky Connie , Iyengar Arun K. S.
Published: July 2024

Direct air capture (DAC) systems that capture carbon dioxide (CO2) directly from the atmosphere are garnering considerable attention for their potential role as negative emission technologies in achieving net-zero CO2 emission goals. Common DAC technologies are based either on liquid–solvent (L-DAC) or solid–sorbent (S-DAC) to capture CO2. A comprehensive multi-factor comparative economic analysis of the deployment of L-DAC and S-DAC facilities across various United States (U.S.) cities is presented in this paper. The analysis considers the influence of various factors on the favorability of DAC deployment, including local climatic conditions such as temperature, humidity, and CO2 concentrations; the availability of energy sources to power the DAC system; and costs for the transport and storage of the captured CO2 along with the consideration of the regional market and policy drivers. The deployment analysis in over 70 continental U.S. cities shows that L-DAC and S-DAC complement each other spatially, as their performance and operational costs vary in different climates. L-DAC is more suited to the hot, humid Southeast, while S-DAC is preferrable in the colder, drier Rocky Mountain region. Strategic deployment based on regional conditions and economics is essential for promoting the commercial adoptability of DAC, which is a critical technology to meet the CO2 reduction targets.
Monthly Hydropower Scheduling of Cascaded Reservoirs Using a Genetic Algorithm with a Simulation Procedure
Authors: Baima Deji , Qian Guoyuan , Luo Jingzhen , Wang Pengcheng , Zheng Hao , Wang Jinwen
Published: July 2024

This study integrates genetic algorithms with simulation programs, applying the genetic algorithm’s (GA) fitness calculation within the simulation to reduce complexity and significantly improve the efficiency of the optimization process. Additionally, the simulation introduces the concept of “Field Leveling” (FL), utilizing a push–pull strategy to explore more space for absorbing and utilizing unnecessary spillage for energy generation, thereby maximizing electricity production and ensuring optimal reservoir scheduling. Two methods are provided, namely the field-leveling genetic algorithms GAFL1 and GAFL2. GAFL1 involves only pushing and does not include a push–pull process; thus, it cannot optimize spillage. On the other hand, GAFL2 implements a complete push–pull strategy, continuously exploring additional space to absorb and utilize unnecessary spillage. Both GAFL1 and GAFL2 achieved reasonable results; specifically, compared to SQP, GAFL1 improved firm yield by 8.3%, spillage increased by 2.2 times, and total energy decreased by 1.2%. GAFL2, building on the basis of GAFL1, effectively reduces spillage under all hydrological conditions without affecting the highest priority of stable output. However, the impact of reducing spillage on energy generation is not consistent; in wet and dry years, reducing spillage increases energy generation. However, in normal years, a reduction in spillage corresponds with decreased energy generation.
Potential of Cation Exchange Resin as a Carrier for Anaerobic Consortia in Biohydrogen Fermentation
Authors: Geng Hui , Xu Ying , Liu Rui , Yang Dianhai , Dai Xiaohu
Published: July 2024

Cation exchange resin (CER) has been reported to promote sludge fermentation. However, previous studies have typically focused on the effects of CER on sludge properties to enhance fermentation, and the role of CER as a biocarrier for anaerobic consortia during fermentation has been overlooked. Thus, in this study, the potential of gel-type and macro-reticular type CERs to serve as biocarriers in fermentation was investigated. A significant number of anaerobes appeared to be attached to the surfaces of CER during 2-day fermentation. However, an extended fermentation time negatively affected the attachment of anaerobic consortia, suggesting that CER may be a suitable carrier for short-term fermentation processes, such as biohydrogen fermentation. Electrochemical analyses revealed that the electron transfer capacities of CER with attached anaerobes were enhanced after both 2-day and 28-day fermentation periods, with the macro-reticular type CER exhibiting higher electron transfer capacity than the gel-type CER. Fermentation experiments using mixing model substrates with macro-reticular and gel-type CERs with attached anaerobes showed that the macro-reticular type CER was more beneficial for biohydrogen fermentation than the gel-type CER. Further analyses of microbial communities revealed that hydrogen-producing bacteria (i.e., Caloramator, unclassified_f_Caloramatoraceace, and Sporanaerobacter) were more likely to adhere to the macro-reticular type CERs. This outcome confirmed that macro-reticular type CERs have significant potential as a carrier for anaerobic consortia to promote the generation of hydrogen and volatile fatty acids. These findings are expected to provide a reference for using materials as biocarriers to enhance the biohydrogen fermentation of sludge.
Characteristics and Functions of Dominant Yeasts Together with Their Applications during Strong-Flavor Baijiu Brewing
Authors: Dong Weiwei , Zeng Yulun , Ma Jiyuan , Cai Kaiyun , Guo Tingting , Tan Guangxun , Yu Xiang , Hu Yuanliang , Peng Nan , Zhao Shumiao
Published: July 2024

Yeasts are pivotal brewing microbes that are associated with the flavor and quality of Chinese baijiu, yet research on dominant yeasts in strong-flavor baijiu brewing remains limited. In this study, Saccharomyces cerevisiae, Pichia kudriavzevii, and Kazachstania bulderi were identified as predominated yeasts in strong-flavor baijiu. Each strain showed distinct characteristics in ethanol resistance, thermal tolerance, and lactic acid tolerance, severally. S. cerevisiae FJ1-2 excelled in ethanol and ethyl ester production, P. kudriavzevii FJ1-1 in ethyl acetate, and K. bulderi FJ1-3 in lactic acid generation. Subsequently, the reinforced Fuqu of each yeast were severally prepared for application in baijiu brewing to verify their functions. Results revealed that the relative abundance of fortified yeast in each group rose. Pichia, Kazachstania, and Saccharomyces emerged as the core microbe for each group, respectively, by co-occurrence network analysis, influencing the microbiota to regulate flavor substances. In short, P. kudriavzevii FJ1-1 enhanced ethyl acetate. K. bulderi FJ1-3 improved ethyl caproate production and decreased levels of ethyl acetate and higher alcohols by modulating yeast community between Pichia and Saccharomyces. This is a systematic endeavor to study the functions of yeasts of strong-flavor baijiu, providing a solid basis for improving baijiu quality.
Nitrogen Gas-Assisted Extrusion for Improving the Physical Quality of Pea Protein-Enriched Corn Puffs with a Wide Range of Protein Contents
Authors: Luo Siwen , Paliwal Jitendra , Koksel Filiz
Published: July 2024

Blowing agent-assisted extrusion cooking is a novel processing technique that can alter the expansion of extruded snacks and, thus, enhance their physical appeal, such as texture. However, to this day, this technique has only been studied for ingredients with limited protein contents (<30%). In this study, protein-enriched snacks were extruded using nitrogen gas as a blowing agent at a wide protein range (0–50%) to better explore the potential of this technique in manufacturing high-protein snacks. The results showed that, with nitrogen gas injection, extrudate radial expansion was significantly (p < 0.05) improved at 10% and 40% protein, while extrudate density was significantly reduced at 30% and 50% protein. Nitrogen gas-injected extrudates, especially at 50% protein, exhibited improvements in texture, including a reduction in hardness and an increase in crispness. Collectively, this study showed the promising potential of nitrogen gas-assisted extrusion in improving the physical appeal of innovative healthy snacks at a high protein level (i.e., 50%).
Protein Hydrolysates from Salmon Heads and Cape Hake By-Products: Comparing Enzymatic Method with Subcritical Water Extraction on Bioactivity Properties
Authors: Pires Carla , Leitão Matilde , Sapatinha Maria , Gonçalves Amparo , Oliveira Helena , Nunes Maria Leonor , Teixeira Bárbara , Mendes Rogério , Camacho Carolina , Machado Manuela , Pintado Manuela , Ribeiro Ana Rita , Vieira Elsa F. , Delerue-Matos Cristina , Lourenço Helena Maria , Marques António
Published: July 2024

Fish by-products can be converted into high-value-added products like fish protein hydrolysates (FPHs), which have high nutritional value and are rich in bioactive peptides with health benefits. This study aims to characterise FPHs derived from salmon heads (HPSs) and Cape hake trimmings (HPHs) using Alcalase for enzymatic hydrolysis and Subcritical Water Hydrolysis (SWH) as an alternative method. All hydrolysates demonstrated high protein content (70.4–88.7%), with the degree of hydrolysis (DH) ranging from 10.7 to 36.4%. The peptide profile of FPHs indicated the breakdown of proteins into small peptides. HPSs showed higher levels of glycine and proline, while HPHs had higher concentrations of glutamic acid, leucine, threonine, and phenylalanine. Similar elemental profiles were observed in both HPHs and HPSs, and the levels of Cd, Pb, and Hg were well below the legislated limits. Hydrolysates do not have a negative effect on cell metabolism and contribute to cell growth. HPSs and HPHs exhibited high 2,2′–azino-bis(3 ethylbenzthiazoline-6)-sulfonic acid (ABTS) radical scavenging activity, Cu2+ and Fe2+ chelating activities, and angiotensin-converting enzyme (ACE) inhibitory activity, with HPHs generally displaying higher activities. The α-amylase inhibition of both FPHs was relatively low. These results indicate that HPHs are a promising natural source of nutritional compounds and bioactive peptides, making them potential candidates for use as an ingredient in new food products or nutraceuticals. SWH at 250 °C is a viable alternative to enzymatic methods for producing FPHs from salmon heads with high antioxidant and chelating properties.
Factors Associated with Uptake of Patient Portals at a Federally Qualified Health Care Center
Authors: Matthews Alicia K. , Steffen Alana D. , Akufo Jennifer , Burke Larisa , Diaz Hilda , Dodd Darcy , Hughes Ashley , Madrid Samantha , Onyiapat Enuma , Opuada Hope , Sejo Jessica , Vilona Brittany , Williams Barbara J. , Donenberg Geri
Published: July 2024

Federally qualified health centers (FQHC) aim to improve cancer prevention by providing screening options and efforts to prevent harmful behavior. Patient portals are increasingly being used to deliver health promotion initiatives. However, little is known about patient portal activation rates in FQHC settings and the factors associated with activation. This study examined patient portal activation among FQHC patients and assessed correlations with demographic, clinical, and health service use variables. We analyzed electronic health record data from adults >18 years old with at least one appointment. Data were accessed from the electronic health records for patients seen between 1 September 2018 and 31 August 2022 (n = 40,852 patients). We used multivariate logistic regression models to examine the correlates of having an activated EPIC-supported MyChart patient portal account. One-third of patients had an activated MyChart portal account. Overall, 35% of patients with an activated account had read at least one portal message, 69% used the portal to schedule an appointment, and 90% viewed lab results. Demographic and clinical factors associated with activation included younger age, female sex, white race, English language, being partnered, privately insured, non-smoking, and diagnosed with a chronic disease. More frequent healthcare visits were also associated with an activated account. Whether or not a patient had an email address in the EHR yielded the strongest association with patient portal activation. Overall, 39% of patients did not have an email address; only 2% of those patients had activated their accounts, compared to 54% of those with an email address. Patient portal activation rates were modest and associated with demographic, clinical, and healthcare utilization factors. Patient portal usage to manage one’s healthcare needs is increasing nationally. As such, FQHC clinics should enhance efforts to improve the uptake and usage of patient portals, including educational campaigns and eliminating email requirements for portal activation, to reinforce cancer prevention efforts.
Computer Simulation of Catheter Cryoablation for Pulmonary Vein Isolation
Authors: Rivera Solange I. , Bernal Clara P. , Martínez-Peláez Rafael , Robledo-Nolasco Rogelio , De León-Larios Gerardo , Félix Vanessa G. , Ostos Rodolfo , Maestre Gladys E. , Melgarejo Jesús D. , Mena Luis J.
Published: July 2024

Cryoablation is a well-established medical procedure for surgically treating atrial fibrillation. Cryothermal catheter therapy induces cellular necrosis by freezing the insides of pulmonary veins, with the goal of disrupting abnormal electrical heart signals. Nevertheless, tissue damage induced by cold temperatures may also lead to other complications after cardiac surgery. In this sense, the simulation of catheter ablation can provide safer environments for training and the performance of cryotherapy interventions. Therefore, in this paper, we propose a novel approach to help better understand how temperature rates can affect this procedure by using computer tools to develop a simulation framework to predict lesion size and determine optimal temperature conditions for reducing the risk of major complications. The results showed that a temperature profile of around −40 °C caused less penetration, reduced necrotic damage, and smaller lesion size in the tissue. Instead, cryotherapy close to −60 °C achieved a greater depth of temperature flow inside the tissue and a larger cross-section area of the lesion. With further development and validation, the framework could represent a cost-effective strategy for providing personalized modeling, better planning of cryocatheter-based treatment, and preventing surgical complications.
Challenges to Viticulture in Montenegro under Climate Change
Authors: Fernandes António , Kovač Nataša , Fraga Hélder , Fonseca André , Šućur Radonjić Sanja , Simeunović Marko , Ratković Kruna , Menz Christoph , Costafreda-Aumedes Sergi , Santos João A.
Published: July 2024

The Montenegrin climate is characterised as very heterogeneous due to its complex topography. The viticultural heritage, dating back to before the Roman empire, is settled in a Mediterranean climate region, located south of the capital Podgorica, where climate conditions favour red wine production. However, an overall increase in warmer and drier periods affects traditional viticulture. The present study aims to discuss climate change impacts on Montenegrin viticulture. Bioclimatic indices, ensembled from five climate models, were analysed for both historical (1981–2010) and future (2041–2070) periods upon three socio-economic pathways: SSP1-2.6, SSP3-7.0 and SSP5-8.5. CHELSA (≈1 km) was the selected dataset for this analysis. Obtained results for all scenarios have shown the suppression of baseline conditions for viticulture. The average summer temperature might reach around 29.5 °C, and the growing season average temperature could become higher than 23.5 °C, advancing phenological events. The Winkler index is estimated to range from 2900 °C up to 3100 °C, which is too hot for viticulture. Montenegrin viticulture requires the application of adaptation measures focused on reducing temperature-increase impacts. The implementation of adaptation measures shall start in the coming years, to assure the lasting productivity and sustainability of viticulture.
Insulin-Activated Signaling Pathway and GLUT4 Membrane Translocation in hiPSC-Derived Cardiomyocytes
Authors: Querio Giulia , Antoniotti Susanna , Levi Renzo , Fleischmann Bernd K. , Gallo Maria Pia , Malan Daniela
Published: July 2024

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are a cell model now widely used to investigate pathophysiological features of cardiac tissue. Given the invaluable contribution hiPSC-CM could make for studies on cardio-metabolic disorders by defining a postnatal metabolic phenotype, our work herein focused on monitoring the insulin response in CM derived from the hiPSC line UKBi015-B. Western blot analysis on total cell lysates obtained from hiPSC-CM showed increased phosphorylation of both AKT and AS160 following insulin treatment, but failed to highlight any changes in the expression dynamics of the glucose transporter GLUT4. By contrast, the Western blot analysis of membrane fractions, rather than total lysates, revealed insulin-induced plasma membrane translocation of GLUT4, which is known to also occur in postnatal CM. Thus, these findings suggest that hiPSC-derived CMs exhibit an insulin response reminiscent to that of adult CMs regarding intracellular signaling and GLUT4 translocation to the plasma membrane, representing a suitable cellular model in the cardio-metabolic research field. Moreover, our studies also demonstrate the relevance of analyzing membrane fractions rather than total lysates in order to monitor GLUT4 dynamics in response to metabolic regulators in hiPSC-CMs.
Allergenic Biomarkers in the Molecular Diagnosis of IgE-Mediated Wheat Allergy
Authors: Preda Mariana , Popescu Florin-Dan , Vassilopoulou Emilia , Smolinska Sylwia
Published: July 2024

IgE-mediated wheat allergy can take on various forms, including childhood food allergy to wheat, wheat-dependent exercise-induced anaphylaxis in young adults, baker’s respiratory allergy/asthma in workers exposed to wheat flour inhalation, and contact urticaria that is caused by hydrolyzed wheat proteins in some cosmetics, and that is sometimes associated with a food allergy. Singleplex and multiplex immunoassays detect specific IgE antibodies to wheat allergenic molecular biomarkers such as omega-5 gliadin Tri a 19, lipid transfer protein Tri a 14, and alpha-amylase inhibitors. The fluorescence enzyme immunoassay with capsulated cellulose polymer solid-phase coupled allergens is a commonly used singleplex assay. Multiplex methods include the ELISA-based macroarray immunoassay using nano-bead technology and a microarray immunoassay on polymer-coated slides. Another promising diagnostic tool is the basophil activation test performed with omega-5 gliadin and other wheat protein types. Detailed comprehension of the structural and immunological features of the numerous wheat allergens significant in clinical settings is imperative for advancing diagnostic biomarkers for IgE-mediated wheat allergies.
Ferroptosis in Arthritis: Driver of the Disease or Therapeutic Option?
Authors: Bieri Shania , Möller Burkhard , Amsler Jennifer
Published: July 2024

Ferroptosis is a form of iron-dependent regulated cell death caused by the accumulation of lipid peroxides. In this review, we summarize research on the impact of ferroptosis on disease models and isolated cells in various types of arthritis. While most studies have focused on rheumatoid arthritis (RA) and osteoarthritis (OA), there is limited research on spondylarthritis and crystal arthropathies. The effects of inducing or inhibiting ferroptosis on the disease strongly depend on the studied cell type. In the search for new therapeutic targets, inhibiting ferroptosis in chondrocytes might have promising effects for any type of arthritis. On the other hand, ferroptosis induction may also lead to a desired decrease of synovial fibroblasts in RA. Thus, ferroptosis research must consider the cell-type-specific effects on arthritis. Further investigation is needed to clarify these complexities.
Perspectives and Possibilities for New Antimicrobial Agents in the Treatment and Control of Mastitis Induced by Algae of the Genus Prototheca spp.: A Review
Authors: Jabłońska Weronika , Gołębiewski Marcin , Kot Magdalena , Mardan Henadzi , Pawliński Bartosz , Kalińska Aleksandra
Published: July 2024

Innovative approaches in nanotechnology provide a potentially promising alternative to untreatable cases of mastitis caused by genus Prototheca spp. algae infections. Drying of the teats of the affected animals or culling are typically the outcomes of mastitis in dairy cattle caused by these pathogens. A major issue in both veterinary medicine and animal breeding is the Prototheca species’ widespread resistance to the current methods of managing infections and the available drugs, including antibiotics. Commercial antifungal preparations are also ineffective. Nanotechnology, an emerging discipline, has the potential to create an effective alternative treatment for protothecal mastitis. The aim of the paper is to combine the literature data on the use of nanotechnology in the control of mastitis, taking into account data on combating mastitis caused by Prototheca spp. infections. The databases employed were PubMed, Google Scholar, and Scopus, focusing on literature from the last 20 years to ensure relevance and currency. Studies conducted in vitro have demonstrated that nanomaterials have significant biocidal activity against mastitis infections of different etiologies. Analyzed research papers show that (NPs), such as AgNPs, CuNPs, AuNPs, etc., may not negatively impact various cell lines and may be effective agents in reducing the pathogens’ viability. However, it is also critical to assess the risks involved in using nanomaterials.
VDR Gene Polymorphisms (BsmI, FokI, TaqI, ApaI) in Total Hip Arthroplasty Outcome Patients
Authors: Rozmus Dominika , Fiedorowicz Ewa , Grzybowski Roman , Płomiński Janusz , Cieślińska Anna
Published: July 2024

A total hip arthroplasty (THA) can improve quality of life, but loosening of the hip prosthesis is a complex problem in which vitamin D may also play a role. The Vitamin D Receptor (VDR) is involved in the response of cells to the action of vitamin D, and its genetic variability raises the question of whether individual differences could influence the risk of prosthesis loosening. The aim of this study was to investigate the relationship between VDR single nucleotide polymorphisms (SNPs) (ApaI, BsmI, FokI and TaqI) and the serum VDR and 25(OH)D levels in three groups of patients: (1) arthroscopy patients after THA without loosening of the prosthesis (CA—Control Arthroplasty), (2) patients after THA with loosened hip prostheses (L—Loosening) and (3) the control group (C—Control). Our results suggest that the genotypes tt of TaqI, BB of BsmI, and FF of FokI may influence the VDR effect in patients with loosened protheses. Our results showed that the ACAC haplotype (AtBF) was over two times more frequent in the L group than in CA + C: OR =2.35 [95% CI 1.44–3.83; p = 0.001]. There was no significant correlation between the VDR and serum 25(OH)D levels, but there were differences between studied groups.
Computational Methods for the Discovery and Optimization of TAAR1 and TAAR5 Ligands
Authors: Scarano Naomi , Espinoza Stefano , Brullo Chiara , Cichero Elena
Published: July 2024

G-protein-coupled receptors (GPCRs) represent a family of druggable targets when treating several diseases and continue to be a leading part of the drug discovery process. Trace amine-associated receptors (TAARs) are GPCRs involved in many physiological functions with TAAR1 having important roles within the central nervous system (CNS). By using homology modeling methods, the responsiveness of TAAR1 to endogenous and synthetic ligands has been explored. In addition, the discovery of different chemo-types as selective murine and/or human TAAR1 ligands has helped in the understanding of the species-specificity preferences. The availability of TAAR1–ligand complexes sheds light on how different ligands bind TAAR1. TAAR5 is considered an olfactory receptor but has specific involvement in some brain functions. In this case, the drug discovery effort has been limited. Here, we review the successful computational efforts developed in the search for novel TAAR1 and TAAR5 ligands. A specific focus on applying structure-based and/or ligand-based methods has been done. We also give a perspective of the experimental data available to guide the future drug design of new ligands, probing species-specificity preferences towards more selective ligands. Hints for applying repositioning approaches are also discussed.
Dynamics of Replication-Associated Protein Levels through the Cell Cycle
Authors: Atemin Aleksandar , Ivanova Aneliya , Kanev Petar-Bogomil , Uzunova Sonya , Nedelcheva-Veleva Marina , Stoynov Stoyno
Published: July 2024

The measurement of dynamic changes in protein level and localization throughout the cell cycle is of major relevance to studies of cellular processes tightly coordinated with the cycle, such as replication, transcription, DNA repair, and checkpoint control. Currently available methods include biochemical assays of cells in bulk following synchronization, which determine protein levels with poor temporal and no spatial resolution. Taking advantage of genetic engineering and live-cell microscopy, we performed time-lapse imaging of cells expressing fluorescently tagged proteins under the control of their endogenous regulatory elements in order to follow their levels throughout the cell cycle. We effectively discern between cell cycle phases and S subphases based on fluorescence intensity and distribution of co-expressed proliferating cell nuclear antigen (PCNA)-mCherry. This allowed us to precisely determine and compare the levels and distribution of multiple replication-associated factors, including Rap1-interacting factor 1 (RIF1), minichromosome maintenance complex component 6 (MCM6), origin recognition complex subunit 1 (ORC1, and Claspin, with high spatiotemporal resolution in HeLa Kyoto cells. Combining these data with available mass spectrometry-based measurements of protein concentrations reveals the changes in the concentration of these proteins throughout the cell cycle. Our approach provides a practical basis for a detailed interrogation of protein dynamics in the context of the cell cycle.
An Overview on MADS Box Members in Plants: A Meta-Review
Authors: Adhikari Prakash Babu , Kasahara Ryushiro Dora
Published: July 2024

Most of the studied MADS box members are linked to flowering and fruit traits. However, higher volumes of studies on type II of the two types so far suggest that the florigenic effect of the gene members could just be the tip of the iceberg. In the current study, we used a systematic approach to obtain a general overview of the MADS box members’ cross-trait and multifactor associations, and their pleiotropic potentials, based on a manually curated local reference database. While doing so, we screened for the co-occurrence of terms of interest within the title or abstract of each reference, with a threshold of three hits. The analysis results showed that our approach can retrieve multi-faceted information on the subject of study (MADS box gene members in the current case), which could otherwise have been skewed depending on the authors’ expertise and/or volume of the literature reference base. Overall, our study discusses the roles of MADS box members in association with plant organs and trait-linked factors among plant species. Our assessment showed that plants with most of the MADS box member studies included tomato, apple, and rice after Arabidopsis. Furthermore, based on the degree of their multi-trait associations, FLC, SVP, and SOC1 are suggested to have relatively higher pleiotropic potential among others in plant growth, development, and flowering processes. The approach devised in this study is expected to be applicable for a basic understanding of any study subject of interest, regardless of the depth of prior knowledge.
Interactions between Dietary Antioxidants, Dietary Fiber and the Gut Microbiome: Their Putative Role in Inflammation and Cancer
Authors: Munteanu Camelia , Schwartz Betty
Published: July 2024

The intricate relationship between the gastrointestinal (GI) microbiome and the progression of chronic non-communicable diseases underscores the significance of developing strategies to modulate the GI microbiota for promoting human health. The administration of probiotics and prebiotics represents a good strategy that enhances the population of beneficial bacteria in the intestinal lumen post-consumption, which has a positive impact on human health. In addition, dietary fibers serve as a significant energy source for bacteria inhabiting the cecum and colon. Research articles and reviews sourced from various global databases were systematically analyzed using specific phrases and keywords to investigate these relationships. There is a clear association between dietary fiber intake and improved colon function, gut motility, and reduced colorectal cancer (CRC) risk. Moreover, the state of health is reflected in the reciprocal and bidirectional relationships among food, dietary antioxidants, inflammation, and body composition. They are known for their antioxidant properties and their ability to inhibit angiogenesis, metastasis, and cell proliferation. Additionally, they promote cell survival, modulate immune and inflammatory responses, and inactivate pro-carcinogens. These actions collectively contribute to their role in cancer prevention. In different investigations, antioxidant supplements containing vitamins have been shown to lower the risk of specific cancer types. In contrast, some evidence suggests that taking antioxidant supplements can increase the risk of developing cancer. Ultimately, collaborative efforts among immunologists, clinicians, nutritionists, and dietitians are imperative for designing well-structured nutritional trials to corroborate the clinical efficacy of dietary therapy in managing inflammation and preventing carcinogenesis. This review seeks to explore the interrelationships among dietary antioxidants, dietary fiber, and the gut microbiome, with a particular focus on their potential implications in inflammation and cancer.
Advancements in Non-Addictive Analgesic Diterpenoid Alkaloid Lappaconitine: A Review
Authors: Zhang Wen , Mi Shujuan , He Xinxin , Cui Jiajia , Zhi Kangkang , Zhang Ji
Published: July 2024

The perennial herb Aconitum sinomontanum Nakai (Ranunculaceae) has been utilized as a traditional oriental medicine in China for numerous years. The principal pharmacological constituent of A. sinomontanum, lappaconitine (LA), exhibits analgesic, anti-inflammatory, anti-tumor, anti-arrhythmic, and anti-epileptic activities. Due to its potent efficacy and non-addictive nature, LA is widely utilized in the management of cancer pain and postoperative analgesia. This review encompasses the research advancements pertaining to LA including extraction methods, separation techniques, pharmacological properties, chemical modifications, and clinical applications. Additionally, it offers insights into the potential applications and current challenges associated with LA to facilitate future research endeavors.
Genome-Wide Analysis and Expression Profiling of Lectin Receptor-like Kinase Genes in Watermelon (Citrullus lanatus)
Authors: Lv Duo , Wang Gang , You Jiaqi , Zhu Lihua , Yang Hongjuan , Cao Biting , Gu Weihong , Li Chaohan
Published: July 2024

Watermelon is one of the most important edible plants worldwide. Owing to its special cultivation conditions, watermelon is exposed to many biological and abiotic stresses during its development. Lectin receptor-like kinases (LecRLKs) are plant-specific membrane proteins that play important roles in sensing and responding to environmental stimuli. Although the LecRLK gene family has been identified in a variety of plants, a comprehensive analysis has not yet been undertaken in watermelon. In this study, 61 putative LecRLK genes were identified in watermelon, consisting of 36 G-type, 24 L-type, and 1 C-type LecRLK genes. They were distributed in clusters on chromosomes, and members from the same subfamily were mostly clustered together. The analysis of the phylogenetic tree and conserved motif indicated that there were obvious differences among three ClaLecRLK subfamilies, and there was also rich diversity in the C-terminal within subfamilies. A collinear analysis revealed that the evolution of the ClaLecRLK gene family in different Cucurbitaceae crops was asynchronous. Furthermore, the analysis of the ClaLecRLK protein structure showed that not all proteins contained signal peptides and a single transmembrane domain. A subcellular localization assay confirmed that the number and position of transmembrane domains did not affect ClaLecRLK protein localization in cells. Transcriptome data revealed distinct expression patterns of LecRLK genes of watermelon in various tissues, and their responses to different fungi infection were also significantly different. Finally, the potential binding sites of the ClaLecRLK genes targeted by miRNA were predicted. This study enhances the understanding of the characteristics and functions of the LecRLK gene family in watermelon and opens up the possibility of exploring the roles that LecRLK genes may play in the life cycle of Cucurbitaceae plants.
Molecular Basis of Influence of A501X Mutations in Penicillin-Binding Protein 2 of Neisseria gonorrhoeae Strain 35/02 on Ceftriaxone Resistance
Authors: Krivitskaya Alexandra V. , Kuryshkina Maria S. , Eremina Maria Y. , Smirnov Ivan V. , Khrenova Maria G.
Published: July 2024

The increase in the resistance of mutant strains of Neisseria gonorrhoeae to the antibiotic ceftriaxone is pronounced in the decrease in the second-order acylation rate constant, k2/KS, by penicillin-binding protein 2 (PBP2). These changes can be caused by both the decrease in the acylation rate constant, k2, and the weakening of the binding affinity, i.e., an increase in the substrate constant, KS. A501X mutations in PBP2 affect second-order acylation rate constants. The PBP2A501V variant exhibits a higher k2/KS value, whereas for PBP2A501R and PBP2A501P variants, these values are lower. We performed molecular dynamic simulations with both classical and QM/MM potentials to model both acylation energy profiles and conformational dynamics of four PBP2 variants to explain the origin of k2/KS changes. The acylation reaction occurs in two elementary steps, specifically, a nucleophilic attack by the oxygen atom of the Ser310 residue and C–N bond cleavage in the β-lactam ring accompanied by the elimination of the leaving group of ceftriaxone. The energy barrier of the first step increases for PBP2 variants with a decrease in the observed k2/KS value. Submicrosecond classic molecular dynamic trajectories with subsequent cluster analysis reveal that the conformation of the β3–β4 loop switches from open to closed and its flexibility decreases for PBP2 variants with a lower k2/KS value. Thus, the experimentally observed decrease in the k2/KS in A501X variants of PBP2 occurs due to both the decrease in the acylation rate constant, k2, and the increase in KS.
Investigation of 2,4-Dihydroxylaryl-Substituted Heterocycles as Inhibitors of the Growth and Development of Biotrophic Fungal Pathogens Associated with the Most Common Cereal Diseases
Authors: Rząd Klaudia , Nucia Aleksandra , Grzelak Weronika , Matysiak Joanna , Kowalczyk Krzysztof , Okoń Sylwia , Matwijczuk Arkadiusz
Published: July 2024

Climate change forces agriculture to face the rapidly growing virulence of biotrophic fungal pathogens, which in turn drives researchers to seek new ways of combatting or limiting the spread of diseases caused by the same. While the use of agrochemicals may be the most efficient strategy in this context, it is important to ensure that such chemicals are safe for the natural environment. Heterocyclic compounds have enormous biological potential. A series of heterocyclic scaffolds (1,3,4-thiadiazole, 1,3-thiazole, 1,2,4-triazole, benzothiazine, benzothiadiazine, and quinazoline) containing 2,4-dihydroxylaryl substituents were investigated for their ability to inhibit the growth and development of biotrophic fungal pathogens associated with several important cereal diseases. Of the 33 analysed compounds, 3 were identified as having high inhibitory potential against Blumeria and Puccinia fungi. The conducted research indicated that the analysed compounds can be used to reduce the incidence of fungal diseases in cereals; however, further thorough research is required to investigate their effects on plant–pathogen systems, including molecular studies to determine the exact mechanism of their activity.
Metabolic Insights into Neuropsychiatric Illnesses and Ketogenic Therapies: A Transcriptomic View
Authors: Sahay Smita , Pulvender Priyanka , Rami Reddy Madhu Vishnu Sankar Reddy , McCullumsmith Robert E. , O’Donovan Sinead M.
Published: July 2024

The disruption of brain energy metabolism, leading to alterations in synaptic signaling, neural circuitry, and neuroplasticity, has been implicated in severe mental illnesses such as schizophrenia, bipolar disorder, and major depressive disorder. The therapeutic potential of ketogenic interventions in these disorders suggests a link between metabolic disturbances and disease pathology; however, the precise mechanisms underlying these metabolic disturbances, and the therapeutic effects of metabolic ketogenic therapy, remain poorly understood. In this study, we conducted an in silico analysis of transcriptomic data to investigate perturbations in metabolic pathways in the brain across severe mental illnesses via gene expression profiling. We also examined dysregulation of the same pathways in rodent or cell culture models of ketosis, comparing these expression profiles to those observed in the disease states. Our analysis revealed significant perturbations across all metabolic pathways, with the greatest perturbations in glycolysis, the tricarboxylic acid (TCA) cycle, and the electron transport chain (ETC) across all three disorders. Additionally, we observed some discordant gene expression patterns between disease states and ketogenic intervention studies, suggesting a potential role for ketone bodies in modulating pathogenic metabolic changes. Our findings highlight the importance of understanding metabolic dysregulation in severe mental illnesses and the potential therapeutic benefits of ketogenic interventions in restoring metabolic homeostasis. This study provides insights into the complex relationship between metabolism and neuropsychiatric disorders and lays the foundation for further experimental investigations aimed at appreciating the implications of the present transcriptomic findings as well as developing targeted therapeutic strategies.