bluuebunny/crossref_metadata_2025 huggingface dataset:
#42 : Comparison of Fertilization, Blastocyst Utilization, Clinical Pregnancy Rate (cPR), Implantation Rate (IR) and On-Going Pregnancy Rate Using Fresh and Frozen-Thawed Micro-Epididymal Sperm Aspiration (MESA) Sperm
Authors: Yong Kai Boon , Heong Chee San , Chew Mee Fong , Tee Sze Tian
Published: January 2024

Background and Aim: To compare the fertilization, blastocyst utilization, clinical pregnancy rate (cPR), implantation rate (IR) and on-going clinical pregnancy rate between fresh versus frozen-thawed micro-epididymal aspirated sperm (MESA) in intra-cytoplasmic sperm injection (ICSI) cycle.

Methods: A retrospective study of a total of 27 MESA-ICSI cycles done from the year 2019 to 2022 was carried out. Patients were categorized into fresh MESA-ICSI group (n=16) and frozen-thawed MESA-ICSI group (n=11). The maternal mean age of both groups was 33.0 and 32.5 respectively. For fresh MESA-ICSI group, retrieved sperm were being injected on the same day while frozen-thawed MESA-ICSI group, sperm sample were being frozen after the MESA procedure and utilized in the future. Sample was processed using sperm medium (COOK MEDICALⓇ). All embryos were cultured in time-lapse monitoring incubator and assessed according to modified Gardner’s grading system. On day 5/6, blastocyst range from grade A to C will be vitrified for frozen embryo transfer (FET). A total of 22 FET procedures were done, and statistical analysis was performed using Fisher’s exact test, p<0.05. The mean number of embryos transferred for MESA-ICSI group and frozen-thawed MESA-ICSI group are 1.43 and 1.13 respectively.

Results: There is no significant difference in the fertilization rate between fresh MESA-ICSI, 71.1% (106/149) and frozen-thawed MESA-ICSI group, 65.4% (70/107). The blastocyst utilization rate between fresh and frozen-thawed MESA-ICSI group are also similar, 45.3% (48/106) and 51.4% (36/70) respectively. Besides, the cPR shown higher in the fresh MESA-ICSI group, 64.3% (9/14) compared to frozen-thawed MESA-ICSI group, 37.5% (3/8). The IR, 50.0% (10/20) and on-going pregnancy rate, 57.1% (8/14) were higher in the fresh MESA-ICSI group compared to frozen-thawed MESA-ICSI group, 33.3% (3/9) and 12.5% (1/8) respectively, p<0.05.

Conclusion: The results show the trend of higher cPR, IR and on-going pregnancy rate in fresh MESA-ICSI group compared to frozen-thawed MESA-ICSI group. However, further study is required to conduct in a larger population in order to obtain a significant result.
#103 : The Clinical Outcomes of Lasered Assisted Hatching on Single Frozen-Thawed Blastocyst Cycle: A Retrospective, Observational Study
Authors: Chong May Kee , Ng Sue Hwee , Sim Poo Keen
Published: January 2024

Background and Aims: The ultimate goal in assisted reproductive field is to achieve a pregnancy. For embryo to implant, embryo must be hatched from zona pellucida. Therefore, pregnancy began. Lasered assisted hatching (LAH) has been proposed as one of the interventions to increase the success rate, yet the efficacy remains uncertain. Hence, the aim of this study was to evaluate the effect of LAH toward clinical outcomes including clinical pregnancy, miscarriage, and ectopic pregnancy rate on single frozen-thawed blastocyst cycle.

Method: A total of 1553 single frozen-thawed blastocyst cycles (LAH, n=116; non-LAH, n=1417) with patient’s mean age of 35 ± 0.06 were recruited from 2018 to 2022. The exclusion criteria were as follows: (a) gamete/embryo donation cycle and (b) PGT cases. Assisted hatching procedure was performed on LAH group with 1.46-[Formula: see text]m noncontact diode laser (LYKOSⓇ Laser) prior to embryo transfer.

Result: The clinical pregnancy, miscarriage and ectopic pregnancy rates for LAH and non-LAH groups were 44.8% versus 48.7%, p = 0.35, 17.3% versus 16.2%, p = 0.86 and 0% versus 0.7%, respectively. No statistically significant difference was found on these clinical outcomes.

Conclusion: This study demonstrated the use of LAH has no impact on the clinical outcomes and did not increase miscarriage and ectopic pregnancy. Therefore, it can be a useful tool for patients with recurrent implantation failure or embryo with thick zona pellucida.
#302 : Shorter Centrifugation Time Leads to Better Sperm Quality in Swim-Up Processing Technique
Authors: Novia Diana , Halim Binarwan
Published: January 2024

Background and Aims: Centrifugation is one of the many factors that cause sperm DNA damage in IVF semen preparation. This can occur due to the formation of reactive oxygen species (ROS) that are uncontrollable, so that the quality of sperm DNA can be damaged. This study aims to compare sperm quality results with swim-up technique under centrifugation times of 5 and 10 minutes.

Method: This study involved 50 patients at the Halim Fertility Center clinic from June 2020 to October 2020. The samples obtained were processed using the swim-up method. Samples were divided into 3 groups, namely control, 5 and 10 minutes of centrifugation. Sperm quality recorded was concentration, total motility, progressive motility and DNA fragmentation index (DFI).

Results: The sperm concentration after 5- and 10-min centrifugation before swim-up (27.78-39.79 and 35.36–51.09, respectively; p [Formula: see text] 0.05) was significantly higher compared to control (24.85–32.33). The total motility before and after 5- and 10-min centrifugation were 43.78–51.08, 97.66–98.20, and 97.86–98.20, respectively. The progressive motility after 5- and 10-min centrifugation (0–41 and 0–54, respectively) was significantly higher than control (0–24; p < 0.05). The DFI was significantly better after 5 min centrifugation (3.82–6.98) compared to control and after 10 min centrifugation (13.48–19.04 and 1–25, respectively; p < 0.05).

Conclusion: Shorter centrifugation times indicate lower progressive motility than long centrifugation times, but considering the lower DFI level, shorter centrifugation times must be used in sperm preparation for IVF to produce better sperm quality.
#178 : Sexual Function in Women from Migrant and Refugee Backgrounds
Authors: Damabi Negin Mirzaei , Avery Jodie , Begum Mumtaz , Padhani Zahra Ali , Lassi Zohra
Published: January 2024

Background and Aims: Women from migrant and refugee backgrounds are poorly studied when it comes to sexual function. These women face multiple barriers to accessing sexual and reproductive health services, including language barriers, socio-cultural beliefs, limited socioeconomic resources, and inadequate knowledge. This study conducted a scoping review of existing literature on sexual function and related knowledge in this population.

Methods: This scoping review adhered to the 2020 PRISMA guidelines and systematically gathered information from online databases such as Medline, Embase, CINAHL, and Cochrane Library. Without applying any search limitations, the review included all studies that examined the sexual function of migrant and refugee women. Based on the eligibility criteria, two authors independently screened and extracted data from the included studies using the Covidence software. Afterwards, descriptive and thematic analyses were conducted.

Results: The findings suggest that the sexual function status of migrants is a complex issue influenced by multiple factors, yet it remains understudied, with the majority of research focusing on sexual and reproductive health (SRH). Studies have shown that migrants may experience higher levels of sexual dysfunction; they tend to have less sexual knowledge, liberal attitudes, and lower rates of desire, arousal, and pleasure compared to non-migrant women. Additionally, factors such as acculturation, education, gender role ideology, and satisfaction with partner relationships can impact their sexual function.

Conclusions: This study underscores the limitations of research on sexual function in migrants. It emphasises the need for culturally sensitive counselling and psychosocially focused programs aimed at addressing the sexual dysfunction inequities experienced by migrant and refugee women and catering to their unique needs in SRH health care sessions. Future research should focus on neglected populations and tailor effective care to enhance their sexual function and overall well-being.
#177 : Pregnancy in Partial Empty Sella Secondary to Infarction of Pituitary Microadenoma
Authors: Raguindin Erica Joy , Manzano Dianne
Published: January 2024

Background and Aims: Hyperprolactinemia results from overstimulation of prolactin production which presents as gonadal dysfunction, cranial symptoms and maybe of different etiologies. The hormonal and somatic changes in pregnancy are predictable and affects all body systems, but the presence of pathologic condition in this altered state may require special attention to account for these changes and the life of the child being carried.

Method: This is a case of a 34-year-old, G4P2(1122) with oligomenorrhea, difficulty in conception and headache. Her first pregnancy last December 2016 ended with an abortion. She was diagnosed with polycystic ovarian syndrome along with hyperprolactinemia. Cranial magnetic resonance imaging (MRI) revealed pituitary microadenoma and was treated with bromocriptine. A successful pregnancy was achieved 2 months after treatment with uneventful course and was lost to follow-up. A spontaneous abortion last December 2020 prompted work-up.

Results: The patient underwent work-up and a repeat laboratory showed a return of hyperprolactinemia but with a partial empty sella on imaging. Resumption of Bromocriptine resulted in remission of prolactin after 2 months. Another successful pregnancy was achieved, and she delivered last 2021 with no complications. She was able to breastfeed postpartum with no subjective complaints. As of writing, the patient is asymptomatic.

Conclusion: A thorough history is a critical step in identifying possible causes which may guide us in further investigations and treatment options for Hyperprolactinemia. A resolution of pituitary adenoma is caused by various pathological processes resulting in partial empty sella. Routine hormonal status assessment and regular follow-up are indicated since the prevalence of pituitary dysfunction is significant.
#332 : A Rare Case Report of Mixed Gonadal Dysgenesis with MOS 46, XY, 47,XY+21, 45,X Karyotype
Authors: Dafhonsa Meycha , Budihastuti Uki Retno , Melinawati Eriana
Published: January 2024

Background and Aims: Mixed gonadal dysgenesis is a condition of unusual and asymmetrical gonadal development leading to an unassigned sex differentiation. A number of differences have been reported in the karyotype, most commonly a mosaicism 45, X/46, XY. We herein report an interesting case of mixed gonadal dysgenesis.

Method: We present a rare patient case with mixed gonadal dysgenesis as a disorder of sex development (DSD) and a new pattern of chromosome in the karyotype, undergoing laparoscopic procedure for sex correction.

Results: A 20-year-old person, reared as female presented with complaints of genital ambiguity and primary amenorrhoea along with lack of secondary sexual characters, but without short stature and Turner’s stigmata.Karyotype analysis revealed Mos 46, XY, 47,XY+21, 45,X karyotypeLuteinizing hormone (LH) was 18.3 mIU/ml and follicle-stimulating hormone (FSH) was 70,07 mIU/ml. Serum estradiol level was 35,9 pg/ml and prolactin was 25,8 ng/ml). Magnetic resonance imaging (MRI) pelvis with contrass (with ultrasonogrphy correlation) revealed uterus hypoplasia with right ovarian-like structures. View of corpus uteri measuring 1× 1.5× 0.8 cm with cervix building length 1 × 0.5 cm were visualized laparoscopically. The right tube and left tube (include bilateral fimbare) appear to be normal in shape and size. Right gonad with base in corpus uteri, white color, elongated ligament-like shape 0.5 × 0.3 × 0.8 cm, no follicle visible. The left gonad was seen with its base at the corpus uteri, white in color and elongated like a ligament uk 0.5 × 0.4 × 0.8cm, no follicles visible. Both gonads biopsied.

Conclusion: Histopathology is still awaiting results. The patient was managed with a multidisciplinary approach and fertility issues discussed with the patient’s family.
#130 : Food Intolerance and Conception in Women Suffering Repeated Implantation Failure
Authors: Lee Li-Hsuan , Hsu Chao Chin
Published: January 2024

Background and Aims: Food-specific immunoglobulins, in combination with the elevated reactive oxygen species and other inflammatory components, have been indicated to represent immunological response to food consumed. This study aimed to investigate the relationship of food intolerance and conception in women receiving IVF treatments.

Method: Infertile women who had experienced at least two failed embryo transfer treatments were investigated between June 2017 and August 2021 in TUBE Fertility Clinic. The whole blood 20 mL was taken from cubital vein and complete food panel examination employing ELISA analyses were designed to assay food-specific IgG and IgE antibodies. The levels of IgG antibodies were presented through spectrophotometric analysis (Table 1). The IgG tests included 5 dairy components, 25 grains/legumes/nuts, 18 vegetables, 9 egg/meat/poultry, 21 fruits, 9 herbs/spices, 18 fish/crustacea/mollusk, and 9 miscellaneous foods including baker’s yeast. Infertile women were asked to refrain from those foods according to the level of hypersensitivity, six months were required for those with reaction class III to IV, and three months for those with reaction class II. Further IVF and embryo transfer cycles were then carried out afterwards.

Results: In total, 67 infertile women enrolled in this study. Their mean age was 38.2 ± 4.3 years old. They had received 2.7 ± 1.6 previous IVF/ET cycles. All 67 women showed positive IgG and 14 (21%) women with positive IgE investigated. Grains/legumes/nuts (90%) and dairy products (86.6%) were the most common hypersensitive foods (Table 2). Thirty-one (46.3%) women conceived in their successive ET cycles after refraining from hypersensitive foods. Lower pregnancy rates were noted in those with hypersensitivity to fish/crustacea/mollusk and eggs/meat/poultry, even after refraining from those foods.

Conclusion: High percentage of infertile women suffering repeated failed IVF/ET cycles presented with different levels of hypersensitivity to foods. Avoidance of hypersensitive foods may help them achieve future conception.
#348 : Primary Broad Ligament Endometrioid Carcinoma – A Case Report of a Rare Entity
Authors: Hamid Habibah Abdul , Rom Maryam Sakinah Mohamad , Jamil Amilia Afzan Mohd , Yahaya Zainab
Published: January 2024

Background: Primary broad ligament epithelial malignancies are rare occurrence. Hence, the treatment options are uncertain. Therefore, an accurate diagnosis is warranted to manage this condition.

Case report: We present a 61-year-old female with abdominal distension associated with significant weight loss for six months. A computed tomography (CT) scan of the abdomen and pelvis revealed a pelvic mass measuring 10.7 × 14.6 × 13.5 cm with omental caking.

Result: An exploratory laparotomy and tumour debulking was performed. Histological examination revealed moderately differentiated endometrioid carcinoma of the broad ligament stage IC and an endometrioid carcinoma of endometrium, stage 1A. She received external beam radiotherapy; brachytherapy followed by adjuvant chemotherapy with a 6 monthly surveillance. Currently, she is healthy with no recurrence at 24 months postoperatively.

Conclusion: The rarity of primary broad ligament endometrioid carcinoma may incline clinicians to suspect more common malignancies such as ovarian. However, with an accurate diagnosis, the treatment will allow a better prognosis to the patient.
#231 : Association Between Reduced Muscle Mass and Obesity on Various Metabolic Risk Factors and Fracture Risk in Mid-Aged Korean Women
Authors: Kim In Hye , Kim So Hee , Yu Eun Hee , Ju Jong Kil
Published: January 2024

Background and Aims: The aim of this study is to investigate effects of reduced muscle mass and obesity on various metabolic risk factors and fractures risk in middle age Korean women.

Method: Retrospectively, the study reviewed medical records of 1,775 female patients who had visited Pusan National University Hospital for routine health screenings from 2010 to 2016. The patients were divided into four groups: group 1, non-sarcopenic, non-obese (NS-NO); group 2, non-sarcopenic, obese (NS-O); group 3, sarcopenic, non-obese (S-NO); and group 4, sarcopenic, obese (S-O) population. Each of the patients was assessed through self-report questionnaires and individual interview with a healthcare provider. The Fracture Risk Algoritham (FRAX) tool was used for bone fracture risk.

Results: Postmenopausal women composed 68.0%, 78.4%, 60.1% and 67.1% in groups 1, 2, 3 and 4, respectively, compared to 68.5% in the overall patients ([Formula: see text]<0.001). Statistical analysis of various parameters for metabolic and cardiovascular risks further showed that more patients tended to have hypertension in S-O group (patient % with hypertension in NS-NO, NS-O, S-NO and S-O groups as 15.2%, 36.7%, 10.1% and 46.3%, respectively; [Formula: see text]<0.001). Metabolic syndrome, type 2 diabetes and hyperlipidemia were more prevalent in NS-O and S-O groups, which was statistically higher in the remaining two groups. FRAX score was also significantly higher in NS-O and S-O groups than NS-NO and S-NO groups (4.2 and 3.9 versus 3.8 and 3.6, respectively; [Formula: see text]=0.001).

Conclusion: When combined with obesity and menopause, patients with reduced muscle mass showed dramatically increased risks to hypertension and diabetes; especially, patients with comorbid obesity and sarcopenia had the highest prevalence of hypertension. Regarding fracture risk, individual cardio-metabolic risks and menopause were more significant than the presence or absence of obesity or sarcopenia.
Market Manipulation of Cryptocurrencies: Evidence from Social Media and Transaction Data
Authors: Li Wen , Bao Lingfeng , Chen Jiachi , Grundy John , Xia Xin , Yang Xiaohu
Published: January 2024

The cryptocurrency market cap has experienced a great increase in recent years. However, large price fluctuations demonstrate the need for governance structures and identify whether there are market manipulations. In this article, we conduct three analyses—social media data analysis, blockchain data analysis, and price bubble analysis—to investigate whether market manipulation exists on Bitcoin, Ethereum, and Dogecoin platforms. Social media data analysis aims to find the reasons for price fluctuations. Blockchain data analysis is used to find detailed behavior of the manipulators. Price bubble analysis is used to investigate the relation between price fluctuation and manipulators’ behavior. By using the three analyses, we show that market manipulation exists on Bitcoin, Ethereum, and Dogecoin. However, market manipulation of Bitcoin is limited, and for most of Bitcoin’s price fluctuations, we found other explanations. The price for Ethereum is the most sensitive to technical updates. Technical companies/teams usually hype some new concepts (e.g., ICO, DeFi), which causes a price spike. The price of Dogecoin has a high correlation with Elon Musk’s X (formerly known as Twitter) activity, showing that influential individuals have the ability to manipulate its prices. In addition, the poor monetary liquidity of Dogecoin allows some users to manipulate its price.
X-FSPMiner: A Novel Algorithm for Frequent Similar Pattern Mining
Authors: Rodríguez-González Ansel Y. , Aranda Ramón , Álvarez-Carmona Miguel Á. , Díaz-Pacheco Angel , Rosas Rosa María Valdovinos
Published: January 2024

Frequent similar pattern mining (FSP mining) allows for finding frequent patterns hidden from the classical approach. However, the use of similarity functions implies more computational effort, necessitating the development of more efficient algorithms for FSP mining. This work aims to improve the efficiency of mining all FSPs when using Boolean and non-increasing monotonic similarity functions. A data structure to condense an object description collection, named
FV-Tree
, and an algorithm for mining all FSPs from the
FV-Tree
, named
X-FSPMiner
, are proposed. The experimental results reveal that the novel algorithm
X-FSPMiner
vastly outperforms the state-of-the-art algorithms for mining all FSPs using Boolean and non-increasing monotonic similarity functions.
Early-life pulmonary viral infection leads to long-term functional and lower airway structural changes in the lungs
Authors: Malinczak Carrie-Anne , Fonseca Wendy , Hrycaj Steven M. , Morris Susan B. , Rasky Andrew J. , Yagi Kazuma , Wellik Deneen M. , Ziegler Steven F. , Zemans Rachel L. , Lukacs Nicholas W.
Published: January 2024

These studies outline a novel finding that early-life respiratory virus infection can alter lung structure and function long-term. Importantly, the data also indicate that there are critical links between inflammatory responses and subsequent events that produce a more severe pathogenic response later in life. The findings provide additional data to support that early-life infections during lung development can alter the trajectory of airway function.
The Effect of a Loading Dose Regimen in the Switch to Brolucizumab for Patients with Aflibercept-Resistant nAMD
Authors: Kamao Hiroyuki , Mitsui Erika , Date Yuto , Goto Katsutoshi , Mizukawa Kenichi , Miki Atsushi
Published: January 2024

Purpose. To evaluate the one-year outcomes of switching to brolucizumab with and without a loading dose regimen (three monthly injections) in eyes with aflibercept-resistant neovascular age-related macular degeneration (nAMD). Methods. We retrospectively studied nAMD patients who had retinal exudate under bimonthly injections of aflibercept and were switched to brolucizumab from aflibercept. Patients were grouped into intravitreal brolucizumab injection (IVBr) with a loading dose regimen (loading group) and without a loading dose regimen (nonloading group). We assessed the best-corrected visual acuity (BCVA), central retinal thickness (CRT) at the fovea, subfoveal choroidal thickness (SFCT), IVBr status (number of injections and last injection interval), and retinal exudate status on optical coherence tomography. Results. Overall, 52 eyes received ≥1 IVBr; 26 eyes received ≥3 IVBr with 12-month follow-up. A total of 13 eyes in the loading group and 13 eyes in the nonloading group were reviewed. One year after switching, BCVA changed from 0.28 ± 0.25 to 0.19 ± 0.28 in the loading group (P=0.28) and from 0.25 ± 0.20 to 0.23 ± 0.25 in the nonloading group (P=0.92). The mean CRT decreased from 263.6 ± 40.7 µm to 221.7 ± 54.6 µm in the loading group (P=0.03), while it only changed from 244.9 ± 77.2 µm to 221.0 ± 78.7 µm in the nonloading group (P=0.26). Both the loading and nonloading groups achieved 69% dry macula. The number of injections received was significantly higher in the loading group (7.6 ± 0.6 vs. 6.8 ± 0.4, P < 0.001). Two patients (4.2%) developed intraocular inflammation. Conclusion. Switching to brolucizumab from aflibercept for eyes with nAMD with resistance to bimonthly injections of aflibercept is a valuable treatment option with and without the loading regimen. This trial is registered with UMIN000023676.
Evaluation of Worker Radiation Exposure during the Kori Unit 1 Steam Generator Dismantling Process
Authors: Kim Hyeon-Ki , Shin Sang-Hwa , Kong Chang-Sig , Kim Chang-Lak
Published: January 2024

Kori Unit 1 was permanently shut down on June 18, 2017. Since then, Korea is actively preparing for the decommissioning of the nuclear power plant. Because decommissioning work is performed in a radioactive environment, worker radiation exposure is a significant consideration. In this study, worker radiation exposure is evaluated during the steam generator, one of the heavy components of nuclear power plant, dismantling process. A radiation evaluation for the dismantling process is performed using the code RESRAD‐BUILD. A steam generator dismantling scenario and optimal cutting method are designed to evaluate worker radiation exposure, considering pipe dimensions, cutting tool speed, and experience in steam generator replacement. The evaluation results are derived for each work type and year. As a result of the evaluation, worker radiation exposure is 7.5 man‐mSv at the year of planned decommissioning.
Machine Learning-Based Integrated Analysis of PANoptosis Patterns in Acute Myeloid Leukemia Reveals a Signature Predicting Survival and Immunotherapy
Authors: Tang Lanlan , Zhang Wei , Zhang Yang , Deng Wenjun , Zhao Mingyi
Published: January 2024

Objective. We conducted a meticulous bioinformatics analysis leveraging expression data of 226 PANRGs obtained from previous studies, as well as clinical data from AML patients derived from the HOVON database. Methods. Through meticulous data analysis and manipulation, we were able to categorize AML cases into two distinct PANRG clusters and subsequently identify differentially expressed genes (PRDEGs) with prognostic significance. Furthermore, we organized the patient data into two corresponding gene clusters, allowing us to investigate the intricate relationship between the risk score, patient prognosis, and the immune landscape. Results. Our findings disclosed significant associations between the identified PANRGs, gene clusters, patient survival, immune system, and cancer-related biological processes and pathways. Importantly, we successfully constructed a prognostic signature comprising nineteen genes, enabling the stratification of patients into high-risk and low-risk groups based on individually calculated risk scores. Furthermore, we developed a robust and practical nomogram model, integrating the risk score and other pertinent clinical features, to facilitate accurate patient survival prediction. Our comprehensive analysis demonstrated that the high-risk group exhibited notably worse prognosis, with the risk score proving to be significantly correlated with infiltration of most immune cells. The qRT-PCR results revealed significant differential expression patterns of LGR5 and VSIG4 in normal and human leukemia cell lines (HL-60 and MV-4-11). Conclusions. Our findings underscore the potential utility of PANoptosis-based molecular clustering and prognostic signatures as predictive tools for assessing patient survival in AML.
Cryptotanshinone Inhibits Bladder Cancer Cell Malignant Progression in a Lipopolysaccharide-Induced Inflammatory Microenvironment through NLRP3 Inhibition
Authors: Tang Chenye , Guo Xiao , Li Yu , Yi Yongxiang , Tang Zhiling , Zhang Qihui , Luo Bairu , Chen Kean , Liang Ke , Li Gang
Published: January 2024

Background. Bladder cancer (BC) is one of the most common malignancies of the urogenital system. This study assessed the nucleotide-binding oligomerization domain and leucine-rich repeat and pyrin domain-containing protein 3 (NLRP3) in BC as well as the effects of cryptotanshinone on changes in BC malignant behaviors and NLRP3 expression under a lipopolysaccharide (LPS)-induced inflammatory microenvironment. Methods. BC tissue specimens from 62 patients were collected for immunohistochemical detection of NLRP3 protein. BC and normal urothelial cell lines were cultured for the detection of NLRP3 mRNA and protein. Then, BC cells were pretreated with LPS to mimic the inflammatory tumor microenvironment. Next, these cells were incubated with a low or high dose of cryptotanshinone to assess its effects on tumor cell malignant behaviors as well as transfected with NLRP3 cDNA to confirm the role of NLRP3 in BC cells in vitro. Results. High NLRP3 expression was associated with larger tumor diameters (>2 cm), muscle invasion, and metastasis. The levels of NLRP3 mRNA and protein were greater in BC cells than in normal urothelial cells. LPS pretreatment significantly promoted NLRP3 and inflammatory cytokine expression in BC cells, and induced cell viability, migration, and invasion. However, cryptotanshinone was able to reduce the LPS-induced increase of NLRP3 and inflammatory cytokine expression as well as the BC cell malignant progression. NLRP3 overexpression using NLRP3 cDNA further promoted BC cell malignant progression after LPS stimulation and reversed cryptotanshinone-reduced LPS-induced BC cell malignant behaviors. Conclusion. NLRP3 might possess oncogenic activity in BC, and the antitumor activity of cryptotanshinone in BC in vitro might be related to its inhibition of NLRP3 expression.
Supplementary Table 2 from Neoadjuvant Selicrelumab, an Agonist CD40 Antibody, Induces Changes in the Tumor Microenvironment in Patients with Resectable Pancreatic Cancer
Authors: Byrne Katelyn T. , Betts Courtney B. , Mick Rosemarie , Sivagnanam Shamilene , Bajor David L. , Laheru Daniel A. , Chiorean E. Gabriela , O'Hara Mark H. , Liudahl Shannon M. , Newcomb Craig , Alanio Cécile , Ferreira Ana P. , Park Byung S. , Ohtani Takuya , Huffman Austin P. , Väyrynen Sara A. , Dias Costa Andressa , Kaiser Judith C. , Lacroix Andreanne M. , Redlinger Colleen , Stern Martin , Nowak Jonathan A. , Wherry E. John , Cheever Martin A. , Wolpin Brian M. , Furth Emma E. , Jaffee Elizabeth M. , Coussens Lisa M. , Vonderheide Robert H.
Published: January 2024

Table S2 summarizes adverse events associated with chemotherapy administration.
Identification of a Biomarker Panel from Genome-Wide Methylation to Detect Early HPV-Associated Oropharyngeal Cancer
Authors: Dickey Brittney L. , Putney Ryan M. , Schell Michael J. , Berglund Anders E. , Amelio Antonio L. , Caudell Jimmy J. , Chung Christine H. , Giuliano Anna R.
Published: January 2024

As oropharyngeal cancer (OPC) associated with human papillomavirus (HPV) increases in men, the need for a screening test to diagnose OPC early is crucial. This study agnostically identified differentially methylated CpG sites to identify additional biomarkers to improve screening for early OPC.

DNA was extracted from oral gargles of 89 early cases and 108 frequency matched healthy controls, and processed for genome-wide methylation using the Illumina Infinium MethylationEPIC BeadChip. Selected sites were combined with our prior methylation data in the EPB41L3 gene (CpG sites 438, 427, and 425) and oral HPV16 and HPV18 status were considered as binary variables (positive/negative). Lasso regression identified CpG sites strongly associated with early OPC. ROC curves with AUC were generated. The panel was validated utilizing bootstrap resampling.

Machine learning analyses identified 14 markers that are significantly associated with early OPC, including one EPB41L3 CpG site (438) and oral HPV16 status. A final model was trained on all available samples using the discovered panel and was able to predict early OPC compared with controls with an AUC of 0.970 on the training set. In the bootstrap validation sets, the average AUC was 0.935, indicating adequate internal validity.

Our data suggest that this panel can detect OPC early, however external validation of this panel is needed. Further refinement of a panel of biomarkers to diagnose OPC earlier is urgently needed to prevent complex treatment of OPC and associated comorbidities, while reducing risk of recurrence.

Prevention Relevance:

This study identified biomarkers using genome-wide methylation to create a panel capable of discerning early oropharyngeal cancer (OPC) from those without OPC. Such a biomarker panel would be an effective tool to detect OPC early and prevent complications of treatment associated with later diagnosis.
Data from The ncBAF Complex Regulates Transcription in AML Through H3K27ac Sensing by BRD9
Authors: Klein David C. , Lardo Santana M. , Hainer Sarah J.
Published: January 2024

Abstract
The non-canonical BAF complex (ncBAF) subunit BRD9 is essential for acute myeloid leukemia (AML) cell viability but has an unclear role in leukemogenesis. Because BRD9 is required for ncBAF complex assembly through its DUF3512 domain, precise bromodomain inhibition is necessary to parse the role of BRD9 as a transcriptional regulator from that of a scaffolding protein. To understand the role of BRD9 bromodomain function in regulating AML, we selected a panel of five AML cell lines with distinct driver mutations, disease classifications, and genomic aberrations and subjected these cells to short-term BRD9 bromodomain inhibition. We examined the bromodomain-dependent growth of these cell lines, identifying a dependency in AML cell lines but not HEK293T cells. To define a mechanism through which BRD9 maintains AML cell survival, we examined nascent transcription, chromatin accessibility, and ncBAF complex binding genome-wide after bromodomain inhibition. We identified extensive regulation of transcription by BRD9 bromodomain activity, including repression of myeloid maturation factors and tumor suppressor genes, while standard AML chemotherapy targets were repressed by inhibition of the BRD9 bromodomain. BRD9 bromodomain activity maintained accessible chromatin at both gene promoters and gene-distal putative enhancer regions, in a manner that qualitatively correlated with enrichment of BRD9 binding. Furthermore, we identified reduced chromatin accessibility at GATA, ETS, and AP-1 motifs and increased chromatin accessibility at SNAIL-, HIC-, and TP53-recognized motifs after BRD9 inhibition. These data suggest a role for BRD9 in regulating AML cell differentiation through modulation of accessibility at hematopoietic transcription factor binding sites.
Significance:
The bromodomain-containing protein BRD9 is essential for AML cell viability, but it is unclear whether this requirement is due to the protein's role as an epigenetic reader. We inhibited this activity and identified altered gene-distal chromatin regulation and transcription consistent with a more mature myeloid cell state.
Is Older Age an Independent Prognostic Factor of Survival in Metastatic Colorectal Cancer?
Authors: Toquero Patricia , Mondéjar Rebeca , Romero-Laorden Nuria , Méndez Elena , Castillo Lucía , Hernández Marín Berta , Donnay Olga , Colomer Ramon
Published: January 2024

Introduction: Older patients (≤75 years) with advanced colorectal cancer (CRC) may have worse survival than non-older patients. We hypothesized that, rather than age alone, concurrent factors may be more relevant for real-world survival. Methods: Patients diagnosed with CRC in a 5-year period (2014–2018) were analyzed to determine which factors influenced in overall survival (OS). Kaplan-Meier method was used to estimate OS. Univariate and multivariate analysis was conducted by Cox regression analysis. The study was approved by Ethics Committee. Results: Out of 477 patients diagnosed with CRC, 231 had advanced disease. Ninety-two patients (40%) were older than 75 years; median OS (mOS) was 17.1 m (95% CI: 14.3–23.3), p < 0.001. In non-older patients, mOS was 26.7 m (95% CI: 21.9–32.6), p < 0.001. We evaluated eighteen concurrent factors that included characteristics related to the patient (age, sex, comorbidities, polypharmacy, Eastern Cooperative Oncology Group (ECOG), and nutritional status), to the tumor (stage at diagnosis, tumor side, molecular profile, tumor burden, location, and number of metastasis), and to the treatment administered (systemic treatment for advanced disease, chemotherapy schedule and number of lines, severe adverse events and dose reductions, and surgery of liver metastasis). In the univariate analysis, age at diagnosis, ECOG, nutritional status, tumor side, molecular profile, tumor burden, systemic treatment for advanced disease, and surgery of liver metastases had significant impact on survival. However, multivariate analysis revealed that only four factors (tumor burden, nutritional status, systemic treatment for advanced disease, and surgery of liver metastases) were independently associated with OS but not older age at diagnosis. Conclusion: Older age is not an independent survival prognostic factor for advanced CRC. Tumor burden, nutritional status, systemic treatment for advanced disease, and surgery of liver metastasis were significant factors associated with OS. These findings suggest that older patients should not be excluded from cancer treatment based on age alone.
Sex-Specific Appetite Regulation of Lipocalin-2 in High-Fat-Diet-Induced Obese Mice
Authors: Miao Linfeng , Tian Chenying , Xiong Qingqing , Zhao Jing , Feng Yunfei , Yu Hong , Du Huahua
Published: January 2024

Introduction: Lipocalin 2 (Lcn2) is a key factor in appetite suppression. However, the effect of Lcn2 on appetite in terms of sex differences has not been thoroughly studied. Methods: Young (3-month-old) whole-body Lcn2 knockout (Lcn2−/−) mice were fed a normal diet (ND) or high-fat diet (HFD) for 8 weeks to investigate obesity, food intake, serum metabolism, hepatic lipid metabolism, and regulation of gastrointestinal hormones. Results: Lcn2 deficiency significantly increased the body weight and food intake of male mice when fed ND instead of HFD and females when fed HFD but not ND. Compared to wild-type (WT) male mice, the adiponectin level and phosphorylated form of adenosine 5′-monophosphate-activated protein kinase (AMPK) in the hypothalamus were both increased in ND-fed Lcn2−/− male mice but decreased in HFD-fed Lcn2−/− male mice. However, in female mice, adiponectin and its energy metabolism pathway were not altered. Instead, estradiol was found to be substantially higher in ND-fed Lcn2−/− female mice and substantially lower in HFD-fed Lcn2−/− female mice compared with WT female mice. Estradiol alteration also caused similar changes in ERα in the hypothalamus, leading to changes in the PI3K/AKT energy metabolism pathway. It suggested that the increased appetite caused by Lcn2 deficiency in male mice may be due to increased adiponectin expression and promotion of AMPK phosphorylation, while in female mice it may be related to the decrease of circulating estradiol and the inhibition of the hypothalamic ERα/PI3K/AKT energy metabolism pathway. Conclusion: Lcn2 plays in a highly sex-specific manner in the regulation of appetite in young mice.
Recent Advances in the Therapeutic Potential of Sinomenine for Cancer Treatment
Authors: Jiang PanZhen , Zahra Aqeela , Guo Xi , Wu Jianping
Published: January 2024

Background: Cancer is a major cause of death worldwide. Although modern medicine has made strides in treatment, a complete cure for cancer remains elusive. Summary: Utilization of medicinal plants in traditional medicine for the treatment of multiple diseases, including cancer, is a well-established practice. Sinomenine is an alkaloid extracted from a medicinal plant and has a diverse range of biological properties, including anti-oxidative, anti-inflammatory, and antibacterial effects. Sinomenine exhibits inhibitory effects on various types of tumor cells, including breast, lung, and liver cancers. The anticancer properties of sinomenine are believed to involve stimulation of apoptosis and autophagy as well as suppression of cell proliferation, invasion, and metastasis. Key Message: This review summarizes the current research on sinomenine’s potential as an anticancer agent, which may contribute to the discovery of more effective cancer treatments.
Os trajes da Igreja Católica: um breve manual de conservação têxtil
Authors:   Universidade de São Paulo. Escola de Comunicações e Artes, Viana Fausto
Published: January 2024

Os trajes da Igreja Católica - um breve manual de conservação têxtil é muito mais do que um livro com sugestões de preservação de trajes, paramentos e linhos de altar usados no rito religioso da Igreja. O livro ensina a higienizar, a guardar, a fazer caixas para proteção dos trajes e peças mais sensíveis que vão garantir a maior duração do patrimônio têxtil - importante do ponto de vista histórico, mas também do ponto afetivo e sentimental dos frequentadores de milhares de paróquias, igrejas e outras associações espalhadas pelo Brasil.
Incidence and Outcomes of New-Onset Right Bundle Branch Block Following Transcatheter Aortic Valve Replacement
Authors: Tan Nicholas Y. , Adedinsewo Demilade , El Sabbagh Abdallah , Sayed Ahmed Ahmed F. , Carolina Morales-Lara Andrea , Wieczorek Mikolaj , Madhavan Malini , Mulpuru Siva K. , Deshmukh Abhishek J. , Asirvatham Samuel J. , Eleid Mackram F. , Friedman Paul A. , Cha Yong-Mei , Killu Ammar M.
Published: January 2024

BACKGROUND:

The incidence and prognosis of right bundle branch block (RBBB) following transcatheter aortic valve replacement (TAVR) are unknown. Hence, we sought to characterize the incidence of post-TAVR RBBB and determine associated risks of permanent pacemaker (PPM) implantation and mortality.

METHODS:

All patients 18 years and above without preexisting RBBB or PPM who underwent TAVR at US Mayo Clinic sites and Mayo Clinic Health Systems from June 2010 to May 2021 were evaluated. Post-TAVR RBBB was defined as new-onset RBBB in the postimplantation period. The risks of PPM implantation (within 90 days) and mortality following TAVR were compared for patients with and without post-TAVR RBBB using Kaplan-Meier analysis and Cox proportional hazards modeling. The risks of PPM implantation (within 90 days) and mortality following TAVR were compared for patients with and without post-TAVR RBBB using Kaplan-Meier analysis and Cox proportional hazards modeling.

RESULTS:

Of 1992 patients, 15 (0.75%) experienced new RBBB post-TAVR. There was a higher degree of valve oversizing among patients with new RBBB post-TAVR versus those without (17.9% versus 10.0%;
P
=0.034). Ten patients (66.7%) with post-TAVR RBBB experienced high-grade atrioventricular block and underwent PPM implantation (median 1 day; Q1, 0.2 and Q3, 4), compared with 268/1977 (13.6%) without RBBB. Following propensity score adjustment for covariates (age, sex, balloon-expandable valve, annulus diameter, and valve oversizing), post-TAVR RBBB was significantly associated with PPM implantation (hazard ratio, 8.36 [95% CI, 4.19–16.7];
P
<0.001). No statistically significant increase in mortality was seen with post-TAVR RBBB (hazard ratio, 0.83 [95% CI, 0.33–2.11];
P
=0.69), adjusting for age and sex.

CONCLUSIONS:

Although infrequent, post-TAVR RBBB was associated with elevated PPM implantation risk. The mechanisms for its development and its clinical prognosis require further study.
A Preservation from Oral and Practiced Rites of Passage in an African Tradition
Authors: Kwakye-Nuako Kwasi
Published: January 2024

This is an originally observed rites of passages in a traditional African context. Through empirical observation and cultural anthropological esoteric engagement, traditions that have been traditionally preserved and kept in the oral or memory have been preserved in the written for the first time. An overview of the Sub-Saharan African context is followed by birthing and naming of the Meru peoples of Kenya. Additional traditions of rites for Meru boys, education, and integration lead to the conclusion.
Public Prayer as Serious Play: Coming-of-Age in Hip Hop Cultures
Authors: Crump Julius
Published: January 2024

Aggrieved religious practitioners often speak to God in public. Their demands of God elicit critical examination not least because they are irreverent and apathetic. The first part of this article explains the importance of hip hop culture to a democratic society through an analysis of Cornel West’s “danceable education”. The second part describes such an education as pious and playful. The next two parts examine how public prayers from a hip hop (part three) and gospel (part four) artist show how questioning God is a socially valuable way to come-of-age. Artists whose vocation is an act of protest against God-forsakenness and poor governance are exemplified in the invocations of those for whom respectability and redemption remain essential. The vocation of invocations in public stress the social value of loss and negation for reasons—maturation and playing—that make public God-talk good for groups that stubbornly need or actively ignore religious practices.
A new subspecies of the subterranean beetle Speoplanes biocovensis Müller, 1934 (Coleoptera: Leiodidae: Cholevinae: Leptodirini) from Bosnia and Herzegovina: the first occurrence of the genus outside Croatia
Authors: VRBICA MAJA , VESOVIĆ NIKOLA , RAĐA TONĆI , ĆURČIĆ SREĆKO
Published: January 2024

A new subterranean leptodirine leiodid beetle, Speoplanes biocovensis bosnicus Vrbica, Vesović, Rađa & Ćurčić, ssp. n., is described and diagnosed from a pit in southern Bosnia and Herzegovina. This is the first discovery of the genus Speoplanes Müller, 1911 in this country and the first discovery outside Croatia. Important morphological features of the new taxon are presented, including aspects of the male and female genitalia. The subspecies is endemic to the Dinaric mountain range of Bosnia and Herzegovina. A key to the identification of taxa of the genus Speoplanes is added.
4. Until George Floyd
Authors: Cuyler Antonio C.
Published: January 2024

The previous chapter advanced discourses about practical solutions to classical music’s exclusion problem relative to women, neurodiverse, and cultural workers from low socioeconomic backgrounds. In Moving Beyond @operaisracist: Exploring Blacktivism as a Pathway to Antiracism and Creative Justice in Opera and (Un)Silencing Blacktivism in Opera: A Conversation about the Letter to the Opera Field from Black Administrators, I document how the Black Opera Alliance and Black Administrators of Opera have compelled opera companies to sign a pledge for racial equity as the first step towards racial justice in classical music post George Floyd’s state-sanctioned murder in May of 2020. The pledge compels opera companies to (1) hire Black artists, (2) require staff, orchestra members, and independent contractors to reflect the racial demographics of our most diverse communities, (3) program and prioritize works by Black composers, (4) hire more Black creatives and production personnel, (5) require that visual artists undergo training in successfully preparing Black artists for the stage, (6) review the organisation’s hiring practices and policies for racism, (7) review the board’s recruitment culture, (7) and include within the company’s official code of conduct a commitment to anti-racism, and anti- oppression. Furthermore, the Black Administrators of Opera suggested that opera companies (1) commit to equity in salaries and promotion opportunities, (2) commit to company-wide racial equity education and professional development, (3) commit to equitable hiring and recruitment practices, (4) commit to company-wide intentional inclusion in the execution of mission and programs, and (5) commit to adequately funding company diversity, equity, and inclusion initiatives and working groups.
7. The Global Conservatoire
Authors: Salazar Diana , Guillaumier Christina
Published: January 2024

This chapter looks beyond surface evaluations of online teaching during the pandemic and considers a new model for online teaching in the conservatoire. We start by recognising recent and significant changes in the external environment for all artists, notably the focus on generating a more sustainable future, the importance (and fragility) of global communities and the role of technology in the arts. While the significance of these aspects for the musician-in-training may not be immediately obvious, we argue that deep engagement with these issues is vital to develop global artistic citizens for the 21st Century. We propose that online teaching in the conservatoire has the potential to become an important vehicle for equipping students to respond to these changes in society. In recognising the possibilities for online teaching, we move from a position of online ‘replacing’ or ‘replicating’ traditional conservatoire teaching to ‘complementing’ practice-led experiences. We examine how core learning and teaching values can be embedded in the online environment to promote synergy between different learning experiences in the conservatoire. Furthermore, we propose that a transnational model of design and delivery could be most effective in developing culturally responsive artists.