bluuebunny/crossref_metadata_2025 huggingface dataset:
Inference of Breed Structure in Farm Animals: Empirical Comparison between SNP and Microsatellite Performance
Authors: Laoun Abbas , Harkat Sahraoui , Lafri Mohamed , Gaouar Semir Bechir Suheil , Belabdi Ibrahim , Ciani Elena , De Groot Maarten , Blanquet Véronique , Leroy Gregoire , Rognon Xavier , Da Silva Anne
Published: January 2020

Knowledge of population structure is essential to improve the management and conservation of farm animal genetic resources. Microsatellites, which have long been popular for this type of analysis, are more and more neglected in favor of whole-genome single nucleotide polymorphism (SNP) chips that are now available for the main farmed animal species. In this study, we compared genetic patterns derived from microsatellites to that inferred by SNPs, considering three pairs of datasets of sheep and cattle. Population genetic differentiation analyses (Fixation index, FST), as well as STRUCTURE analyses showed a very strong consistency between the two types of markers. Microsatellites gave pictures that were largely concordant with SNPs, although less accurate. The best concordance was found in the most complex dataset, which included 17 French sheep breeds (with a Pearson correlation coefficient of 0.95 considering the 136 values of pairwise FST, obtained with both types of markers). The use of microsatellites reduces the cost and the related analyses do not require specific computer equipment (i.e., information technology (IT) infrastructure able to provide adequate computing and storage capacity). Therefore, this tool may still be a very appropriate solution to evaluate, in a first stage, the general state of livestock at national scales. At a time when local breeds are disappearing at an alarming rate, it is urgent to improve our knowledge of them, in particular by promoting tools accessible to the greatest number.
Biodosimetry of Low Dose Ionizing Radiation Using DNA Repair Foci in Human Lymphocytes
Authors: Jakl Lukáš , Marková Eva , Koláriková Lucia , Belyaev Igor
Published: January 2020

Purpose: Ionizing radiation induced foci (IRIF) known also as DNA repair foci represent most sensitive endpoint for assessing DNA double strand breaks (DSB). IRIF are usually visualized and enumerated with the aid of fluorescence microscopy using antibodies to γH2AX and 53BP1. This study analyzed effect of low dose ionizing radiation on residual IRIF in human lymphocytes to the aim of potential biodosimetry and possible extrapolation of high-dose γH2AX/53BP1 effects to low doses and compared kinetics of DSB and IRIF. We also analyzed whether DNaseI, which is used for reducing of clumps, affects the IRIF level. Materials and Methods: The cryopreserved human lymphocytes from umbilical cord blood (UCB) were thawed with/without DNaseI, γ-irradiated at doses of 0, 5, 10, and 50 cGy and γH2AX/53BP1 foci were analyzed 30 min, 2 h, and 22 h post-irradiation using appropriate antibodies. We also analyzed kinetics of DSB using PFGE. Results: No significant difference was observed between data obtained by γH2AX foci evaluation in cells that were irradiated by low doses and data obtained by extrapolation from higher doses. Residual 53BP1 foci induced by low doses significantly outreached the data extrapolated from irradiation by higher doses. 53BP1 foci induced by low dose-radiation remain longer at DSB loci than foci induced by higher doses. There was no significant effect of DNaseI on DNA repair foci. Conclusions: Primary γH2AX, 53BP1 foci and their co-localization represent valuable markers for biodosimetry of low doses, but their usefulness is limited by short time window. Residual γH2AX and 53BP1 foci are more useful markers for biodosimetry in vitro. Effects of low doses can be extrapolated from high dose using γH2AX residual foci while γH2AX/53BP1 foci are valuable markers for evaluation of initial DSB induced by ionizing radiation. Residual IRIF induced by low doses persist longer time than those induced by higher doses.
A Clinical Diagnostic System for Late-Stage Neuropsychiatric Lyme Borreliosis Based upon an Analysis of 100 Patients
Authors: Bransfield Robert C. , Aidlen Dylan M. , Cook Michael J. , Javia Sagar
Published: January 2020

Many late-stage chronic Lyme disease clinical findings are neuropsychiatric. A total clinical assessment is critical in diagnosis, especially since controversy surrounds the reliability of laboratory testing. The clinical findings of one hundred Lyme disease patients with chronic neuropsychiatric symptoms were entered into a database. The prevalence of each clinical finding pre-infection and post-infection was compared and calculated within the 95% confidence interval. Patients had minimal symptoms pre-infection, but a high post-infection prevalence of a broad spectrum of acquired multisystem symptoms. These findings included impairments of attention span, memory, processing, executive functioning, emotional functioning, behavior, psychiatric syndromes, vegetative functioning, neurological, musculoskeletal, cardiovascular, upper respiratory, dental, pulmonary, gastrointestinal, genitourinary, and other symptoms. The most prevalent symptoms included sustained attention impairments, brain fog, unfocused concentration, joint symptoms, distraction by frustration, depression, working memory impairments, decreased school/job performance, recent memory impairments, difficulty prioritizing multiple tasks, fatigue, non-restorative sleep, multitasking difficulties, sudden mood swings, hypersomnia, mental apathy, decreased social functioning, insomnia, tingling, word finding difficulties, name retrieval, headaches, sound hypersensitivity, paresis, anhedonia, depersonalization, cold intolerance, body temperature fluctuations, light sensitivity and dysfluent speech. The average patient had five symptoms pre-infection and 82 post-infection. Pattern recognition is critical in making a diagnosis. This study was used to develop three clinical assessment forms.
Dynamic Correlation Analysis Method of Air Pollutants in Spatio-Temporal Analysis
Authors: Bai Yu-ting , Jin Xue-bo , Wang Xiao-yi , Wang Xiao-kai , Xu Ji-ping
Published: January 2020

Pollutant analysis and pollution source tracing are critical issues in air quality management, in which correlation analysis is important for pollutant relation modeling. A dynamic correlation analysis method was proposed to meet the real-time requirement in atmospheric management. Firstly, the spatio-temporal analysis framework was designed, in which the process of data monitoring, correlation calculation, and result presentation were defined. Secondly, the core correlation calculation method was improved with an adaptive data truncation and grey relational analysis. Thirdly, based on the general framework and correlation calculation, the whole algorithm was proposed for various analysis tasks in time and space, providing the data basis for ranking and decision on pollutant effects. Finally, experiments were conducted with the practical data monitored in an industrial park of Hebei Province, China. The different pollutants in multiple monitoring stations were analyzed crosswise. The dynamic features of the results were obtained to present the variational correlation degrees from the proposed and contrast methods. The results proved that the proposed dynamic correlation analysis could quickly acquire atmospheric pollution information. Moreover, it can help to deduce the influence relation of pollutants in multiple locations.
Personality Traits or Genetic Determinants—Which Strongly Influences E-Cigarette Users?
Authors: Grzywacz Anna , Suchanecka Aleksandra , Chmielowiec Jolanta , Chmielowiec Krzysztof , Szumilas Kamila , Masiak Jolanta , Balwicki Łukasz , Michałowska-Sawczyn Monika , Trybek Grzegorz
Published: January 2020

Presently, a growing popularity of electronic cigarettes may be observed. Used as a means of obtaining nicotine they allow to substitute traditional cigarettes. The origins of substance use disorders are conditioned by dopaminergic signaling which influences motivational processes being elementary factors conditioning the process of learning and exhibiting goal-directed behaviors. The study concentrated on analysis of three polymorphisms located in the dopamine receptor 2 (DRD2) gene—rs1076560, rs1799732 and rs1079597 using the PCR method, personality traits determined with the Big Five Questionnaire, and anxiety measured with the State Trait Anxiety Inventory. The study was conducted on a group of 394 volunteers, consisting e-cigarette users (n = 144) and controls (n = 250). Compared to the controls the case group subjects achieved significantly higher scores in regard to the STAI state and the trait scale, as well as the NEO-FFI Neuroticism and Openness scale. Likewise, in the case of the STAI state for DRD2 rs1076560 significant differences were found. Furthermore, while comparing the groups (e-cigarette users vs. controls) we noticed interactions for the NEO FFI Neuroticism and DRD2 rs1076560. The same was observed in the case of interactions significance while comparing groups (e-cigarette users vs. controls) for the STAI trait/scale and DRD2 rs1799732. Findings from this study demonstrate that psychological factors and genetic determinants should be analyzed simultaneously and comprehensively while considering groups of addicted patients. Since the use, and rapid increase in popularity, of electronic cigarettes has implications for public health, e-cigarette users should be studied holistically, especially younger groups of addicted and experimenting users.
Why Are Women Prone to Restless Legs Syndrome?
Authors: Seeman Mary V.
Published: January 2020

Restless legs syndrome is a relatively common neurologic disorder considerably more prevalent in women than in men. It is characterized by an inactivity-induced, mostly nocturnal, uncomfortable sensation in the legs and an urge to move them to make the disagreeable sensation disappear. Some known genes contribute to this disorder and the same genes contribute to an overlapping condition—periodic leg movements that occur during sleep and result in insomnia. Dopamine and glutamate transmission in the central nervous system are involved in the pathophysiology, and an iron deficiency has been shown in region-specific areas of the brain. A review of the literature shows that pregnant women are at particular risk and that increased parity is a predisposing factor. Paradoxically, menopause increases the prevalence and severity of symptoms. This implies a complex role for reproductive hormones. It suggests that changes rather than absolute levels of estrogen may be responsible for the initiation of symptoms. Both iron (at relatively low levels in women) and estrogen (at relatively high oscillating levels in women) influence dopamine and glutamate transmission, which may help to explain women’s vulnerability to this condition. The syndrome is comorbid with several disorders (such as migraine, depression, and anxiety) to which women are particularly prone. This implies that the comorbid condition or its treatment, or both, contribute to the much higher prevalence in women than in men of restless legs syndrome.
SHMP-Amended Ca-Bentonite/Sand Backfill Barrier for Containment of Lead Contamination in Groundwater
Authors: Yang Yu-Ling , Reddy Krishna R. , Zhang Wen-Jie , Fan Ri-Dong , Du Yan-Jun
Published: January 2020

This study investigated the feasibility of using sodium hexametaphosphate (SHMP)- amended calcium (Ca) bentonite in backfills for slurry trench cutoff walls for the containment of lead (Pb) contamination in groundwater. Backfills composed of 80 wt% sand and 20 wt% either Ca-bentonite or SHMP-amended Ca-bentonite were tested for hydraulic conductivity and sorption properties by conducting laboratory flexible-wall hydraulic conductivity tests and batch isothermal sorption experiments, respectively. The results showed that the SHMP amendment causes a one order of magnitude decrease in hydraulic conductivity of the backfill using tap water (1.9 to 3.0 × 10−10 m/s). Testing using 1000 mg/L Pb solution resulted insignificant variation in hydraulic conductivity of the amended backfill. Moreover, SHMP-amendment induced favorable conditions for increased sorption capacity of the backfill, with 1.5 times higher retardation factor relative to the unamended backfill. The Pb transport modeling through an hypothetical 1-m-thick slurry wall composed of amended backfill revealed 12 to 24 times of longer breakthrough time for Pb migration as compared to results obtained for the same thickness slurry wall with unamended backfill, which is attributed to decrease in seepage velocity combined with increase in retardation factor of the backfill with SHMP amendment. Overall, SHMP is shown to be a promising Ca-bentontie modifier for use in backfill for slurry trench cutoff wall for effective containment of Pb-contaminated groundwater.
Medication Adherence, Burden and Health-Related Quality of Life in Adults with Predialysis Chronic Kidney Disease: A Prospective Cohort Study
Authors: Tesfaye Wubshet H. , McKercher Charlotte , Peterson Gregory M. , Castelino Ronald L. , Jose Matthew , Zaidi Syed Tabish R. , Wimmer Barbara C.
Published: January 2020

This study examines the associations between medication adherence and burden, and health-related quality of life (HRQOL) in predialysis chronic kidney disease (CKD). A prospective study targeting adults with advanced CKD (estimated glomerular filtration rate (eGFR) < 30 mL/min/1.73 m2) and not receiving renal replacement therapy was conducted in Tasmania, Australia. The actual medication burden was assessed using the 65-item Medication Regimen Complexity Index, whereas perceived burden was self-reported using a brief validated questionnaire. Medication adherence was assessed using a four-item Morisky-Green-Levine Scale (MGLS) and the Tool for Adherence Behaviour Screening (TABS). The Kidney Disease and Quality of Life Short-Form was used to assess HRQOL. Of 464 eligible adults, 101 participated in the baseline interview and 63 completed a follow-up interview at around 14 months. Participants were predominantly men (67%), with a mean age of 72 (SD 11) years and eGFR of 21 (SD 6) mL/min/1.73 m2. Overall, 43% and 60% of participants reported medication nonadherence based on MGLS and TABS, respectively. Higher perceived medication burden and desire for decision-making were associated with nonadherent behaviour. Poorer HRQOL was associated with higher regimen complexity, whereas nonadherence was associated with a decline in physical HRQOL over time. Medication nonadherence, driven by perceived medication burden, was prevalent in this cohort, and was associated with a decline in physical HRQOL over time.
Evaluation on Early Drought Warning System in the Jinghui Channel Irrigation Area
Authors: Lu Shibao , Shang Yizi , Zhang Hongbo
Published: January 2020

With the economic growth, continuous global environment deterioration, and increasingly serious water resources shortage, droughts have become more and more serious and produced great impacts on both the regional ecology and sustainable economic development. This paper has established the “green, blue, yellow, orange, and red lights” as the early warning grades for agricultural droughts. By using the two influencing factors, namely precipitation and soil moisture, this paper has established the drought assessment index evaluation model using weighted coupling method. It has carried out the analogue simulation of the early drought warning based on the Jinghui Channel’s 2013 water source situations. The soil moisture in January and February is relatively ideal, and the actual early drought warning is expressed by the “green light”. The soil moisture deficit is comparatively serious in March, but the situation concerning water inflow is ideal with the “green light”. Actually, the early warning signal is basically consistent with the soil moisture drought degree between April and August. The actual early warning is expressed by the “green light” as well, but the soil moisture is not so ideal, however, this is the seeding time of the winter wheat so the lack of soil moisture has no impact on the crops output. In November and December, the winter wheat is at the growth and development stage and does not need much moisture. At this stage, the soil moisture is relatively poor. By integrating the time effects into the early drought warning system, this paper provides administrators of irrigation areas with a scientific decision-making based on the drought control measures.
Presentation and Verification of an Optimal Operating Scheme Aiming at Reducing the Ground Vibration Induced by High Dam Flood Discharge
Authors: Lian Jijian , Chen Lin , Liang Chao , Liu Fang
Published: January 2020

Ground and environmental vibrations induced by high dam flood discharge from the Xiangjiaba hydropower station (XHS) has significant adverse effects on nearby building safety and the physical and mental health of surrounding residents. As an effective approach to simulate the flow-induced vibration of hydraulic structures, the hydro-elastic experiment approach has been extensively applied and researched by Chinese scholars, but the relevant systematic research is rarely reported in international journals. Firstly, the hydraulic and structural dynamic similarity conditions that should be satisfied by the hydro-elastic model are briefly reviewed and derived. A hydro-elastic model of the XHS was further constructed using self-developed high-density rubber, and the vibration isolation system (including open trenches and flexible connects) was applied to avoid the external disturbances of pump operation, vehicle vibration and other experiments in the laboratory. Based on the data of model and prototype dynamic tests, a back propagation (BP) neural network was established to map the acceleration of the physical model to the ground in the prototype. In order to reduce the ground vibration, experiments were carried out to meticulously evaluate the ground vibration intensity under more than 600 working conditions, and the optimal operation scheme under different discharge volumes is presented here in detail. According to the prototype test data in 2013, 2014, and 2015, ground vibrations were significantly reduced by applying the presented optimal operation principle which indicates that the presented hydro-elastic approach and the vibration attenuation operation scheme were effective and feasible.
Curcumin Can Decrease Tissue Inflammation and the Severity of HSV-2 Infection in the Female Reproductive Mucosa
Authors: Vitali Danielle , Bagri Puja , Wessels Jocelyn M. , Arora Meenakshi , Ganugula Raghu , Parikh Ankit , Mandur Talveer , Felker Allison , Garg Sanjay , Kumar M.N.V. Ravi , Kaushic Charu
Published: January 2020

Herpes Simplex Virus Type 2 (HSV-2) is one of the most prevalent sexually transmitted viruses and is a known risk factor for HIV acquisition in the Female Genital Tract (FGT). Previously, we found that curcumin can block HSV-2 infection and abrogate the production of inflammatory cytokines and chemokines by genital epithelial cells in vitro. In this study, we investigated whether curcumin, encapsulated in nanoparticles and delivered by various in vivo routes, could minimize inflammation and prevent or reduce HSV-2 infection in the FGT. Female mice were pre-treated with curcumin nanoparticles through oral, intraperitoneal and intravaginal routes, and then exposed intravaginally to the tissue inflammation stimulant CpG-oligodeoxynucleotide (ODN). Local intravaginal delivery of curcumin nanoparticles, but not intraperitoneal or oral delivery, reduced CpG-mediated inflammatory histopathology and decreased production of pro-inflammatory cytokines Interleukin (IL)-6, Tumor Necrosis Factor Alpha (TNF-α) and Monocyte Chemoattractant Protein-1 (MCP-1) in the FGT. However, curcumin nanoparticles did not demonstrate anti-viral activity nor reduce tissue pathology when administered prior to intravaginal HSV-2 infection. In an alternative approach, intravaginal pre-treatment with crude curcumin or solid dispersion formulations of curcumin demonstrated increased survival and delayed pathology following HSV-2 infection. Our results suggest that curcumin nanoparticle delivery in the vaginal tract could reduce local tissue inflammation. The anti-inflammatory properties of curcumin delivered to the vaginal tract could potentially reduce the severity of HSV-2 infection and decrease the risk of HIV acquisition in the FGT of women.
Exploring the Brassinosteroid Signaling in Monocots Reveals Novel Components of the Pathway and Implications for Plant Breeding
Authors: Gruszka Damian
Published: January 2020

Brassinosteroids (BRs) are a class of steroidal phytohormones which are key regulators of diverse processes during whole life cycle of plants. Studies conducted in the dicot model species Arabidopsis thaliana have allowed identification and characterization of various components of the BR signaling. It is currently known that the BR signaling is interconnected at various stages with other phytohormonal and stress signaling pathways. It enables a rapid and efficient adaptation of plant metabolism to constantly changing environmental conditions. However, our knowledge about mechanism of the BR signaling in the monocot species is rather limited. Thus, identification of new components of the BR signaling in monocots, including cereals, is an ongoing process and has already led to identification of some monocot-specific components of the BR signaling. It is of great importance as disturbances in the BR signaling influence architecture of mutant plants, and as a consequence, the reaction to environmental conditions. Currently, the modulation of the BR signaling is considered as a target to enhance yield and stress tolerance in cereals, which is of particular importance in the face of global climate change.
Oxidative Stress-Responsive MicroRNAs in Heart Injury
Authors: Kura Branislav , Szeiffova Bacova Barbara , Kalocayova Barbora , Sykora Matus , Slezak Jan
Published: January 2020

Reactive oxygen species (ROS) are important molecules in the living organisms as a part of many signaling pathways. However, if overproduced, they also play a significant role in the development of cardiovascular diseases, such as arrhythmia, cardiomyopathy, ischemia/reperfusion injury (e.g., myocardial infarction and heart transplantation), and heart failure. As a result of oxidative stress action, apoptosis, hypertrophy, and fibrosis may occur. MicroRNAs (miRNAs) represent important endogenous nucleotides that regulate many biological processes, including those involved in heart damage caused by oxidative stress. Oxidative stress can alter the expression level of many miRNAs. These changes in miRNA expression occur mainly via modulation of nuclear factor erythroid 2-related factor 2 (Nrf2), sirtuins, calcineurin/nuclear factor of activated T cell (NFAT), or nuclear factor kappa B (NF-κB) pathways. Up until now, several circulating miRNAs have been reported to be potential biomarkers of ROS-related cardiac diseases, including myocardial infarction, hypertrophy, ischemia/reperfusion, and heart failure, such as miRNA-499, miRNA-199, miRNA-21, miRNA-144, miRNA-208a, miRNA-34a, etc. On the other hand, a lot of studies are aimed at using miRNAs for therapeutic purposes. This review points to the need for studying the role of redox-sensitive miRNAs, to identify more effective biomarkers and develop better therapeutic targets for oxidative-stress-related heart diseases.
Exosomes for Diagnosis and Therapy in Gastrointestinal Cancers
Authors: Scavo Maria Principia , Depalo Nicoletta , Tutino Valeria , De Nunzio Valentina , Ingrosso Chiara , Rizzi Federica , Notarnicola Maria , Curri Maria Lucia , Giannelli Gianluigi
Published: January 2020

Exosomes are membrane-bound extracellular vesicles (EVs) released by most cells, having a size ranging from 30 to 150 nm, and are involved in mechanisms of cell-cell communication in physiological and pathological tissues. Exosomes are engaged in the transport of biomolecules, such as lipids, proteins, messenger RNAs, and microRNA, and in signal transmission through the intercellular transfer of components. In the context of proteins and nucleic acids transported from exosomes, our interest is focused on the Frizzled proteins family and related messenger RNA. Exosomes can regenerate stem cell phenotypes and convert them into cancer stem cells by regulating the Wnt pathway receptor family, namely Frizzled proteins. In particular, for gastrointestinal cancers, the Frizzled protein involved in those mechanisms is Frizzled-10 (FZD-10). Currently, increasing attention is being devoted to the protein and lipid composition of exosomes interior and membranes, representing profound knowledge of specific exosomes composition fundamental for their application as new delivering drug tools for cancer therapy. This review intends to cover the most recent literature on the use of exosome vesicles for early diagnosis, follow-up, and the use of these physiological nanovectors as drug delivery systems for gastrointestinal cancer therapy.
Profiling of O-acetylated Gangliosides Expressed in Neuroectoderm Derived Cells
Authors: Cavdarli Sumeyye , Yamakawa Nao , Clarisse Charlotte , Aoki Kazuhiro , Brysbaert Guillaume , Le Doussal Jean-Marc , Delannoy Philippe , Guérardel Yann , Groux-Degroote Sophie
Published: January 2020

The expression and biological functions of oncofetal markers GD2 and GD3 were extensively studied in neuroectoderm-derived cancers in order to characterize their potential as therapeutic targets. Using immunological approaches, we previously identified GD3, GD2, and OAcGD2 expression in breast cancer (BC) cell lines. However, antibodies specific for O-acetylated gangliosides are not exempt of limitations, as they only provide information on the expression of a limited set of O-acetylated ganglioside species. Consequently, the aim of the present study was to use structural approaches in order to apprehend ganglioside diversity in melanoma, neuroblastoma, and breast cancer cells, focusing on O-acetylated species that are usually lost under alkaline conditions and require specific analytical procedures. We used purification and extraction methods that preserve the O-acetyl modification for the analysis of native gangliosides by MALDI-TOF. We identified the expression of GM1, GM2, GM3, GD2, GD3, GT2, and GT3 in SK-Mel28 (melanoma), LAN-1 (neuroblastoma), Hs 578T, SUM 159PT, MDA-MB-231, MCF-7 (BC), and BC cell lines over-expressing GD3 synthase. Among O-acetylated gangliosides, we characterized the expression of OAcGM1, OAcGD3, OAcGD2, OAcGT2, and OAcGT3. Furthermore, the experimental procedure allowed us to clearly identify the position of the sialic acid residue that carries the O-acetyl group on b- and c-series gangliosides by MS/MS fragmentation. These results show that ganglioside O-acetylation occurs on both inner and terminal sialic acid residue in a cell type-dependent manner, suggesting different O-acetylation pathways for gangliosides. They also highlight the limitation of immuno-detection for the complete identification of O-acetylated ganglioside profiles in cancer cells.
ColEval: Honeybee COLony Structure EVALuation for Field Surveys
Authors: Hernandez Julie , Maisonnasse Alban , Cousin Marianne , Beri Constance , Le Quintrec Corentin , Bouetard Anthony , Castex David , Decante Damien , Servel Eloïs , Buchwalder Gerald , Brunet François , Feschet-Destrella Estelle , de Bellescize Kiliana , Kairo Guillaume , Frontero Léa , Pédehontaa-Hiaa Miren , Buisson Robin , Pouderoux Theo , Aebi Alexandre , Kretzschmar André
Published: January 2020

Methods for the evaluation and comparison of the structure of numerous honeybee colonies are needed for the development of applied and fundamental field research, as well as to evaluate how the structure and activity of honeybee colonies evolve over time. ColEval complements existing methods, as it uses an online reference image bank for (human) learning and training purposes. ColEval is based on the evaluation of the surface area percentage occupied by different components of a honeybee colony: adult worker bees, open and capped brood, honey, nectar, and pollen. This method is an essential tool for the description of the evolution in the size of honeybee colonies. The procedure makes allowances for tendencies between different observers and uses them to calculate accurate measurements of honeybee colony evaluation. ColEval thus allows for a posteriori comparison of under- or over-evaluation made by different observers working on the same project; it is thus possible to eliminate observer bias in the measurements and to conduct large surveys.
Comparative Effectiveness of Chuna Manipulative Therapy for Non-Acute Lower Back Pain: A Multi-Center, Pragmatic, Randomized Controlled Trial
Authors: Park Sun-Young , Hwang Eui-Hyoung , Cho Jae-Heung , Kim Koh-Woon , Ha In-Hyuk , Kim Me-riong , Nam Kibong , Lee Min ho , Lee Jun-Hwan , Kim Namkwen , Shin Byung-Cheul
Published: January 2020

Current evidence on the effectiveness and safety of Chuna manipulative therapy (CMT) for managing non-acute lower back pain (LBP) is insufficient. We investigated the comparative effectiveness and safety of CMT, a Korean style of manipulation, plus usual care (UC) compared to UC alone for non-acute LBP. We conducted a parallel, two-armed, multi-centered, assessor blinded, pragmatic, randomized controlled trial at four major Korean medical hospitals. Overall, 194 patients were randomly allocated to either CMT plus UC (n = 97) or UC alone (n = 97), for six weeks of treatment and six months follow-up. The primary outcome was measured using the numerical rating scale (NRS) of LBP intensity at 7 weeks. Secondary outcomes included NRS of leg pain, Oswestry Disability Index (ODI) for functional disability, patient global impression of change (PGIC) scale, and safety. A total of 194 patients were included in the intention-to-treat analysis, and 174 patients provided complete data for the primary outcome. At 7 weeks, clinically significant differences between groups were observed in the NRS of LBP (CMT + UC: −3.02 ± 1.72, UC: −1.36 ± 1.75, p < 0.001), ODI scores (CMT + UC: −5.65 ± 4.29, UC: −3.72 ± 4.63, p = 0.003), NRS of leg pain (CMT + UC: −2.00 ± 2.33, UC: −0.44 ± 1.86, p < 0.0001), and PGIC (CMT + UC: −0.28 ± 0.85, UC: 0.01 ± 0.66, p = 0.0119). Mild to moderate safety concerns were reported in 21 subjects. CMT plus UC showed higher effectiveness compared to UC alone in patients with non-acute LBP in reducing LBP and leg pain and in improving function with good safety results using a powered sample size and including mid-term follow-up.
Admission Hyperglycemia as a Predictor of Mortality in Acute Heart Failure: Comparison between the Diabetics and Non-Diabetics
Authors: Cho Jae Yeong , Kim Kye Hun , Lee Sang Eun , Cho Hyun-Jai , Lee Hae-Young , Choi Jin-Oh , Jeon Eun-Seok , Kim Min-Seok , Kim Jae-Joong , Hwang Kyung-Kuk , Chae Shung Chull , Baek Sang Hong , Kang Seok-Min , Choi Dong-Ju , Yoo Byung-Su , Ahn Youngkeun , Park Hyun-Young , Cho Myeong-Chan , Oh Byung-Hee
Published: January 2020

Background: To investigate the impact of admission hyperglycemia (HGL) on in-hospital death (IHD) and 1-year mortality in acute heart failure (AHF) patients with or without diabetes mellitus (DM). Methods: Among 5625 AHF patients enrolled in a nationwide registry, 5541 patients were divided into four groups based on the presence of admission HGL and diabetes mellitus (DM). Admission HGL was defined as admission glucose level > 200 mg/dL. IHD and 1-year mortality were compared. Results: IHD developed in 269 patients (4.9%), and 1-year death developed in 1220 patients (22.2%). DM was a significant predictor of 1-year death (24.8% in DM vs. 20.5% in non-DM, p < 0.001), but not for IHD. Interestingly, admission HGL was a significant predictor of both IHD (7.6% vs. 4.2%, p < 0.001) and 1-year death (26.2% vs. 21.3%, p = 0.001). Admission HGL was a significant predictor of IHD in both DM and non-DM group, whereas admission HGL was a significant predictor of 1-year death only in non-DM (27.8% vs. 19.9%, p = 0.003), but not in DM group. In multivariate analysis, admission HGL was an independent predictor of 1-year mortality in non-DM patients (HR 1.32, 95% CI 1.03–1.69, p = 0.030). Conclusion: Admission HGL was a significant predictor of IHD and 1-year death in patients with AHF, whereas DM was only a predictor of 1-year death. Admission HGL was an independent predictor of 1-year mortality in non-DM patients with AHF, but not in DM patients. Careful monitoring and intensive medical therapy should be considered in AHF patients with admission HGL, regardless of DM.
Hemodynamic Predictors for Sepsis-Induced Acute Kidney Injury: A Preliminary Study
Authors: Antal Oana , Ștefănescu Elena , Mleșnițe Monica , Bălan Andrei Mihai , Caziuc Alexandra , Hagău Natalia
Published: January 2020

The aim of our study was to assess the association between the macrohemodynamic profile and sepsis induced acute kidney injury (AKI). We also investigated which minimally invasive hemodynamic parameters may help identify patients at risk for sepsis-AKI. We included 71 patients with sepsis and septic shock. We performed the initial fluid resuscitation using local protocols and continued to give fluids guided by the minimally invasive hemodynamic parameters. We assessed the hemodynamic status by transpulmonary thermodilution technique. Sequential organ failure assessment (SOFA score) (AUC 0.74, 95% CI 0.61–0.83, p < 0.01) and cardiovascular SOFA (AUC 0.73, 95% CI 0.61–0.83, p < 0.01) were found to be predictors for sepsis-induced AKI, with cut-off values of 9 and 3 points respectively. Persistent low stroke volume index (SVI) ≤ 32 mL/m2/beat (AUC 0.67, 95% CI 0.54–0.78, p < 0.05) and global end-diastolic index (GEDI) < 583 mL/m2 (AUC 0.67, 95% CI 0.54–0.78, p < 0.05) after the initial fluid resuscitation are predictive for oliguria/anuria at 24 h after study inclusion. The combination of higher vasopressor dependency index (VDI, calculated as the (dobutamine dose × 1 + dopamine dose × 1 + norepinephrine dose × 100 + vasopressin × 100 + epinephrine × 100)/MAP) and norepinephrine, lower systemic vascular resistance index (SVRI), and mean arterial blood pressure (MAP) levels, in the setting of normal preload parameters, showed a more severe vasoplegia. Severe vasoplegia in the first 24 h of sepsis is associated with a higher risk of sepsis induced AKI. The SOFA and cardiovascular SOFA scores may identify patients at risk for sepsis AKI. Persistent low SVI and GEDI values after the initial fluid resuscitation may predict renal outcome.
Temperature Compensation Circuit for ISFET Sensor
Authors: Gaddour Ahmed , Dghais Wael , Hamdi Belgacem , Ben Ali Mounir
Published: January 2020

PH measurements are widely used in agriculture, biomedical engineering, the food industry, environmental studies, etc. Several healthcare and biomedical research studies have reported that all aqueous samples have their pH tested at some point in their lifecycle for evaluation of the diagnosis of diseases or susceptibility, wound healing, cellular internalization, etc. The ion-sensitive field effect transistor (ISFET) is capable of pH measurements. Such use of the ISFET has become popular, as it allows sensing, preprocessing, and computational circuitry to be encapsulated on a single chip, enabling miniaturization and portability. However, the extracted data from the sensor have been affected by the variation of the temperature. This paper presents a new integrated circuit that can enhance the immunity of ion-sensitive field effect transistors (ISFET) against the temperature. To achieve this purpose, the considered ISFET macro model is analyzed and validated with experimental data. Moreover, we investigate the temperature dependency on the voltage-current (I-V). Accordingly, an improved conditioning circuit is designed in order to reduce the temperature sensitivity on the measured pH values of the ISFET sensor. The numerical validation results show that the developed solution accurately compensates the temperature variation on the measured pH values at low power consumption.
Marine Application of Fiber Reinforced Composites: A Review
Authors: Rubino Felice , Nisticò Antonio , Tucci Fausto , Carlone Pierpaolo
Published: January 2020

Components and structures working in the marine environment are exposed to high stresses attributable to the action of wind, waves, and tides. Moreover, they have to face hostile and severe environmental conditions during their lifetime, being placed in the splash zone if not even submerged in saltwater. The application of polymer composites in marine systems has been the focus of intensive studies in the last decades, highlighting potential benefits given by the replacement of several components, such as ship hulls, propeller blades, wind, and tidal turbine blades, to cite but a few. The present paper reports the latest advances in this area, addressing the applications of advanced composites in ships and ship components, offshore oil and gas composites, marine renewable energy and underwater repairing.
Ultradeep Microbial Communities at 4.4 km within Crystalline Bedrock: Implications for Habitability in a Planetary Context
Authors: Purkamo Lotta , Kietäväinen Riikka , Nuppunen-Puputti Maija , Bomberg Malin , Cousins Claire
Published: January 2020

The deep bedrock surroundings are an analog for extraterrestrial habitats for life. In this study, we investigated microbial life within anoxic ultradeep boreholes in Precambrian bedrock, including the adaptation to environmental conditions and lifestyle of these organisms. Samples were collected from Pyhäsalmi mine environment in central Finland and from geothermal drilling wells in Otaniemi, Espoo, in southern Finland. Microbial communities inhabiting the up to 4.4 km deep bedrock were characterized with phylogenetic marker gene (16S rRNA genes and fungal ITS region) amplicon and DNA and cDNA metagenomic sequencing. Functional marker genes (dsrB, mcrA, narG) were quantified with qPCR. Results showed that although crystalline bedrock provides very limited substrates for life, the microbial communities are diverse. Gammaproteobacterial phylotypes were most dominant in both studied sites. Alkanindiges -affiliating OTU was dominating in Pyhäsalmi fluids, while different depths of Otaniemi samples were dominated by Pseudomonas. One of the most common OTUs detected from Otaniemi could only be classified to phylum level, highlighting the uncharacterized nature of the deep biosphere in bedrock. Chemoheterotrophy, fermentation and nitrogen cycling are potentially significant metabolisms in these ultradeep environments. To conclude, this study provides information on microbial ecology of low biomass, carbon-depleted and energy-deprived deep subsurface environment. This information is useful in the prospect of finding life in other planetary bodies.
Compliant Micron-Sized Patterned InGaN Pseudo-Substrates Utilizing Porous GaN
Authors: Pasayat Shubhra S. , Gupta Chirag , Wang Yifan , DenBaars Steven P. , Nakamura Shuji , Keller Stacia , Mishra Umesh K.
Published: January 2020

The compliant behavior of densely packed 10 × 10 µm2 square patterned InGaN layers on top of porous GaN is demonstrated. The elastic relaxation of the InGaN layers is enabled by the low stiffness of the porous GaN under layer. High resolution X-ray diffraction measurements show that upon InGaN re-growths on these InGaN-on-porous GaN pseudo-substrates, not only was the regrown layer partially relaxed, but the degree of relaxation of the InGaN pseudo-substrate layer on top of the porous GaN also showed an increase in the a-lattice constant. Furthermore, methods to improve the surface morphology of the InGaN layers grown by metal-organic chemical vapor deposition (MOCVD) were explored in order to fabricate InGaN pseudo-substrates for future optoelectronic and electronic devices. The largest a-lattice constant demonstrated in this study using this improved method was 3.209 Å, corresponding to a fully relaxed InGaN film with an indium composition of 0.056.
The Effect of the ZrO2 Loading in SiO2@ZrO2-CaO Catalysts for Transesterification Reaction
Authors: Salinas Daniela , Guerrero Sichem , Campos Cristian H. , Bustamante Tatiana M. , Pecchi Gina
Published: January 2020

The effect of the ZrO2 loading was studied on spherical SiO2@ZrO2-CaO structures synthetized by a simple route that combines the Stöber and sol-gel methods. The texture of these materials was determined using SBET by N2 adsorption, where the increment in SiO2 spheres’ surface areas was reached with the incorporation of ZrO2. Combined the characterization techniques of using different alcoholic dissolutions of zirconium (VI) butoxide 0.04 M, 0.06 M, and 0.08 M, we obtained SiO2@ZrO2 materials with 5.7, 20.2, and 25.2 wt % of Zr. Transmission electron microscopy (TEM) analysis also uncovered the shape and reproducibility of the SiO2 spheres. The presence of Zr and Ca in the core–shell was also determined by TEM. X-ray diffraction (XRD) profiles showed that the c-ZrO2 phase changed in to m-ZrO2 by incorporating calcium, which was confirmed by Raman spectroscopy. The purity of the SiO2 spheres, as well as the presence of Zr and Ca in the core–shell, was assessed by the Fourier transform infrared (FTIR) method. CO2 temperature programmed desorption (TPD-CO2) measurements confirmed the increment in the amount of the basic sites and strength of these basic sites due to calcium incorporation. The catalyst reuse in FAME production from canola oil transesterification allowed confirmation that these calcium core@shell catalysts turn out to be actives and stables for this reaction.
Interface-Dominated Time-Dependent Behavior of Poled Poly(Vinylidene Fluoride–Trifluoroethylene)/Barium Titanate Composites
Authors: Dalle Vacche Sara , Damjanovic Dragan , Michaud Véronique , Leterrier Yves
Published: January 2020

Composites in which particles of ferroelectric ceramic phase are randomly dispersed in a polymeric matrix are of interest because of flexibility, conformability, and ease of processing. However, their piezoelectric properties are rather low, unless very high volume fractions of ceramics are used. This brings agglomeration and porosity issues due to the large mismatch between the surface energies of the ceramics and of the polymer. Particle surface modification is a common approach for better dispersion; however, it may bring other effects on the properties of the composites, which are usually concealed by the huge improvement in performance due to the more homogenous microstructure. In this work, we compared poly(vinylidene fluoride–trifluoroethylene)/barium titanate composites containing 15 vol.% and 60 vol.% of pristine ceramic particles or particles modified with an aminosilane or a fluorosilane. Similar morphology, with good particle dispersion and low porosity, was achieved for all composites, owing to an efficient dispersion method. The materials were poled with two different poling procedures, and the piezoelectric coefficient d33, the relative permittivity, and the poling degree of barium titanate were followed in time. We highlighted that, although similar d33 were obtained with all types of particles, the nature of the particles surface and the poling procedure were associated with different charge trapping and influenced the evolution of d33 with time.
Properties of Concrete Containing Recycled Glass Aggregates Produced of Exploded Lighting Materials
Authors: Drzymała Tomasz , Zegardło Bartosz , Tofilo Piotr
Published: January 2020

The paper presents an analysis of the possibilities of using glass waste from recycled lighting materials as aggregates for cement concrete. The research material was obtained from a company that utilizes electrical waste. Glass from pre-sorted elements was transported to the laboratory and crushed in a drum crusher. In this way, the aggregate obtained was subjected to the basic tests that are carried out for aggregates traditionally used in construction. The specific density of aggregate, bulk density, absorbability, crushing index, grain shape, texture type and aggregate flatness index were examined. In the next stage of research work, concrete mixtures were made in which crushed aggregate from crushed fluorescent lamps was used as a substitute for gravel aggregate. Mixtures containing 10%, 30%, 50% and 100% aggregate were made. A mixture containing only sand and gravel aggregate was made as a comparative mixture. Basic tests of both fresh concrete mix and hardened concrete were carried out for all concrete made. The consistency of the fresh concrete mix, the air content in the concrete mix, the density of hardened concrete, absorbability, water permeability under pressure and the basic compressive and tensile (flexular) strength tests were performed. The test results showed that the greater the addition of recycled glass aggregate, the less advantageous are the features of the concrete obtained with its participation. Microscopic analyses carried out in order to explain this phenomenon indicated an unfavorable influence of the grain shape of the aggregate thus obtained. Despite this fact, recycling of lighting waste in concrete composites is recommended as a pro-ecology measure; however, attention was paid to the benefits of using only 30% by mass of said waste in relation to the weight of the traditional aggregate used. Composite with such a quantity of waste retained the characteristics of cement concrete, which qualified its use for construction concrete.
Titania-Coated Alumina Foam Photocatalyst for Memantine Degradation Derived by Replica Method and Sol-Gel Reaction
Authors: Švagelj Zrinka , Mandić Vilko , Ćurković Lidija , Biošić Martina , Žmak Irena , Gaborardi Mattia
Published: January 2020

In the present work, alumina (Al2O3) foam was prepared by the replica method where a polyurethane (PU) foam (30 pores per inch (ppi)) template was impregnated with a 60 wt.% Al2O3 suspension. Sintered Al2O3 foam was used as substrate for the deposition of sol-gel derived titania (TiO2) film using dip coating. For the preparation of TiO2 sol, titanium(IV) isopropoxide (Ti-iPrOH) was used as the precursor. The common problem of qualification and quantification of a crystalline coating on a highly porous 3D substrate with an uneven surface was addressed using a combination of different structural characterization methods. Using Powder X-ray Diffraction (PXRD) and synchrotron Grazing Incidence X-ray Diffraction (GIXRD) on bulk and powdered Al2O3 foam and TiO2-coated Al2O3 foam samples, it was determined Al2O3 foam crystallizes to corundum and coating to anatase, which was also confirmed by Fourier Transformed Infrared Spectroscopy (FTIR). Scanning Electron Microscopy with Energy Dispersive X-ray Spectroscopy (SEM/EDS) revealed the structural and microstructural properties of the substrate and coating. Differential Thermal Analysis (DTA) and Thermogravimetric Analysis (TGA) were used to clarify the evolution of the porous microstructure. The Al2O3-TiO2 composite was evaluated as a photocatalyst candidate for the degradation of the micropollutant medication memantine. The degradation rate was monitored using a light-emitting diode (LED) lamp operating at electromagnetic (EM) wavelength of 365 nm. The photocatalytic activity of sol-gel-derived TiO2 film immobilized on the Al2O3 foam was compared with commercially available TiO2 nanoparticles, P25-Degussa, in the form of a suspension. The levels of memantine were monitored by High-Performance Liquid Chromatography–Tandem Mass Spectrometry (HPLC–MS/MS). The efficiency and rate of the memantine photodegradation by suspended TiO2 nanoparticles is higher than the TiO2-coated Al2O3 foam. But, from the practical point of view, TiO2-coated Al2O3 foam is more appropriate as a valuable photocatalytic composite material.