Surveillance of HIV-1 primary infections in France from 2014 to 2016: toward stable resistance, but higher diversity, clustering and virulence?
Authors: Visseaux Benoit , Assoumou Lambert , Mahjoub Nadia , Grude Maxime , Trabaud Mary-Anne , Raymond Stéphanie , Wirden Marc , Morand-Joubert Laurence , Roussel Catherine , Montes Brigitte , Bocket Laurence , Fafi-Kremer Samira , Amiel Corinne , De Monte Anne , Stefic Karl , Pallier Coralie , Tumiotto Camille , Maillard Anne , Vallet Sophie , Ferre Virginie , Bouvier-Alias Magali , Dina Julia , Signori-Schmuck Anne , Carles Marie-Josée , Plantier Jean-Christophe , Meyer Laurence , Descamps Diane , Chaix Marie-Laure ,   AC43 ANRS Resistance Group, Roussel C , Le Guillou-Guillemette H , Ducancelle A , Courdavault L , Alloui C , Honore P , Lepiller Q , Bettinger D , Bellecave P , Pinson-Recordon P , Tumiotto C , Vallet S , Payan C , Duthe J C , Leroux M , Dina J , Vabret A , Mirand A , Henquell C , Bouvier-Alias M , Simohamed A , Dos Santos G , Yerly S , Gaille C , Caveng W , Chapalay S , Calmy A , Signori-Schmuck A , Morand P , Pallier C , Raho-Moussa M , Mole M , Dulucq M-J , Bocket L , Alidjinou K , Ranger-Rogez S , Trabaud M A , Icard V , Tardy J C , Tamalet C , Delamare C , Montes B , Schvoerer E , Fenaux H , Rodallec A , André-Garnier E , Ferré V , De Monte A , Guigon A , Guinard J , Descamps D , Charpentier C , Visseaux B , Peytavin G , Tremaux P , Avettand-Fenoel V , Soulié C , Malet I , Wirden M , Marcelin A G , Calvez V , Flandre P , Assoumou L , Costagliola D , Morand-Joubert L , Lambert-Niclot S , Fofana D , Boukli N , Delaugerre C , Chaix M L , Mahjoub N , Amiel C , Giraudeau G , Beby-Defaux A , Plainchamp D , Maillard A , Alessandri-Gradt E , Leoz M , Plantier J C , Gantner P , Delagreverie H , Fafi-Kremer S , Fischer P , Raymond S , Izopet J , Chiabrando J , Stefic K , Barin F , Fajole G , Burgault O , Marque-Juillet S 
Published: August 2019

Objectives

Patients with primary HIV-1 infection (PHI) are a particular population, giving important insight about ongoing evolution of transmitted drug resistance-associated mutation (TDRAM) prevalence, HIV diversity and clustering patterns. We describe these evolutions of PHI patients diagnosed in France from 2014 to 2016.

Methods

A total of 1121 PHI patients were included. TDRAMs were characterized using the 2009 Stanford list and the French ANRS algorithm. Viral subtypes and recent transmission clusters (RTCs) were also determined.

Results

Patients were mainly MSM (70%) living in the Paris area (42%). TDRAMs were identified among 10.8% of patients and rose to 18.6% when including etravirine and rilpivirine TDRAMs. Prevalences of PI-, NRTI-, first-generation NNRTI-, second-generation NNRTI- and integrase inhibitor-associated TDRAMs were 2.9%, 5.0%, 4.0%, 9.4% and 5.4%, respectively. In a multivariable analysis, age >40 years and non-R5 tropic viruses were associated with a >2-fold increased risk of TDRAMs. Regarding HIV diversity, subtype B and CRF02_AG (where CRF stands for circulating recombinant form) were the two main lineages (56% and 20%, respectively). CRF02_AG was associated with higher viral load than subtype B (5.83 versus 5.40 log10 copies/mL, P = 0.004). We identified 138 RTCs ranging from 2 to 14 patients and including overall 41% from the global population. Patients in RTCs were younger, more frequently born in France and more frequently MSM.

Conclusions

Since 2007, the proportion of TDRAMs has been stable among French PHI patients. Non-B lineages are increasing and may be associated with more virulent CRF02_AG strains. The presence of large RTCs highlights the need for real-time cluster identification to trigger specific prevention action to achieve better control of the epidemic.