bluuebunny/crossref_metadata_2025 huggingface dataset:
Simulating the Effects of Drought Stress Timing and the Amount Irrigation on Cotton Yield Using the CSM-CROPGRO-Cotton Model
Authors: Wang Lei , Lin Meiwei , Han Zhenxiang , Han Lianjin , He Liang , Sun Weihong
Published: December 2023

Drought stress disrupts the molecular-level water balance in plants, and severe water deficiency can be fatal for cotton plants. However, mild water deficits or short-term drought stress may enhance crop resilience, increasing yields. The present study aims to determine the optimal watering time and irrigation amount to induce drought tolerance in cotton seedlings during drought training. Specifically, the investigation focuses on identifying the ideal day for watering and the corresponding irrigation volume that effectively triggers the transition of cotton plants into a state of enhanced resistance to drought stress during the seedling stage. In this study, the CSM-CROPGRO-Cotton model was utilized, and our objectives were to (i) evaluate the predictive capability of CSM-CROPGRO-Cotton for yield estimation in field experiments in Xinjiang and (ii) simulate and assess the range of time during the seedling stage when cotton plants can withstand drought stress without reducing yields, identifying irrigation strategies that induce drought training while maintaining yield under mild water deficiency. The model was validated using yield data from field experiments conducted in 2023. The validation criteria included a normalized root mean square error (nRMSE)>10% and a coefficient of determination (r2)>85% for yield; for the leaf area index (LAI), the criterion was (r2)>90%, with a degree of agreement of (d)>75%. The results demonstrated the accuracy of the CSM-CROPGRO-Cotton model in predicting cotton yield. Based on the validated CSM-CROPGRO-Cotton model, this study employed the LINUX crop model batch-processing technique to efficiently simulate 357 different irrigation strategies by adjusting the amount of “first irrigation” and timing. The findings revealed that in the irrigation scheme for cotton during the seedling stage, when the amount of first irrigation was in the lower range of 10 mm to 15 mm, the cotton plants underwent drought training during the early growth stage, and their yields did not exhibit drastic fluctuations due to reduced amounts of first irrigation. The suitable period for first irrigation for drought training was from 25 June to 6 July, and the amount of first irrigation could save approximately 57.14% in irrigation water. This implies that subjecting cotton plants to a certain level of drought training can enhance their stress tolerance and increase yields. This finding holds great significance for cotton cultivation in drought-prone regions.
Dosage Optimisation of Trimethoprim and Sulfamethoxazole for the Treatment of an Avian Pathogenic Strain of Escherichia coli in Broiler Chickens
Authors: Stastny Kamil , Hodkovicova Nikola , Jerabek Martin , Petren Michal , Viskova Michaela , Papouskova Aneta , Bartejsova Iva , Putecova-Tosnerova Kristina , Charvatova Michaela , Zouharova Monika , Matiaskova Katarina , Nedbalcova Katerina
Published: December 2023

Based on pharmacokinetic studies carried out according to the methodologies defined by the European Medicines Agency (EMA) using mass spectrometry analysis, a new formulation of a veterinary drug for the treatment of broiler chickens is proposed. Currently, the traditional trimethoprim–sulfamethoxazole drug used for broilers is applied in a 1:5 ratio, and the recommended dose is 45 mg kg−1 of live weight administered at 24 h intervals for 3 to 5 days. In this study, we propose a novel combination containing similar active substances in a newly established ratio of 1:4, with a recommended dosage of 20 mg kg−1 of live weight administered at 24 h intervals for 3 to 5 days. With this method, the currently recommended dose of the traditional trimethoprim–sulfamethoxazole drug used for broilers can be reduced by more than half. The efficacy of the newly designed formulation and dosage of the drug was verified in a bioassay for the treatment of broilers experimentally infected with an avian pathogenic strain of Escherichia coli. In the experiment, we compared the newly designed dosage with the traditional dosage in terms of efficacy and dosage. There were no statistically significant differences between the two drugs in efficacy regarding the survival of chickens after experimental infection or changes in their health status. The experimental results suggest that a significant reduction in the recommended daily dose of drugs containing trimethoprim and sulfamethoxazole for the treatment of bacterial infections in broilers is possible and can support the prudent use of antimicrobials, including the limitation of their overuse.
Development of Acridone Derivatives: Targeting c-MYC Transcription in Triple-Negative Breast Cancer with Inhibitory Potential
Authors: Liang Jing-Wei , Gao Zhi-Chao , Yang Lu-Lu , Zhang Wei , Chen Ming-Zhe , Meng Fan-Hao
Published: December 2023

Breast cancer, especially the aggressive triple-negative subtype, poses a serious health threat to women. Unfortunately, effective targets are lacking, leading to a grim prognosis. Research highlights the crucial role of c-MYC overexpression in this form of cancer. Current inhibitors targeting c-MYC focus on stabilizing its G-quadruplex (G4) structure in the promoter region. They can inhibit the expression of c-MYC, which is highly expressed in triple-negative breast cancer (TNBC), and then regulate the apoptosis of breast cancer cells induced by intracellular ROS. However, the clinical prospects for the application of such inhibitors are not promising. In this research, we designed and synthesized 29 acridone derivatives. These compounds were assessed for their impact on intracellular ROS levels and cell activity, followed by comprehensive QSAR analysis and molecular docking. Compound N8 stood out, significantly increasing ROS levels and demonstrating potent anti-tumor activity in the TNBC cell line, with excellent selectivity shown in the docking results. This study suggests that acridone derivatives could stabilize the c-MYC G4 structure. Among these compounds, the small molecule N8 shows promising effects and deserves further investigation.
Accumulation Patterns of Metabolites Responsible for the Functional Quality of Virgin Olive Oil during Olive Fruit Ontogeny
Authors: Luaces Pilar , Expósito Jesús , Benabal Paula , Pascual Mar , Sanz Carlos , Pérez Ana G.
Published: December 2023

The health-promoting antioxidant properties of virgin olive oil (VOO) are today considered priority targets in the new olive breeding programs. Given that these properties depend mainly on its phenolic fraction, whose origin lies in the phenolic compounds present in olive fruit, the objective of this study was to provide further insight into the accumulation dynamics of the main antioxidant compounds, including both polar phenolics and lipophilic tocopherols, during the ontogeny of the olive fruit. Data obtained show that, albeit with significant differences, all the studied genotypes share just after fruit set an intense increase in the synthesis of tyrosol and hydroxytyrosol derivatives, by far the main phenolic compounds of the olive fruit, and a subsequent steady decrease along fruit development and ripening. The accumulation dynamics of flavonoids and tocopherols were different from those of tyrosol and hydroxytyrosol derivatives, presenting a peak of synthesis just before the onset of fruit ripening, and then in general, their content decreases throughout the ripening phase. In the case of flavonoids, all genotypes also share a strong increase in the accumulation of anthocyanins in the final stages of fruit ripening, coinciding with the change in fruit color. Furthermore, the results during the fruit ripening process evidenced that the content of tyrosol and hydroxytyrosol derivatives and tocopherols in the fruit largely determines the content of these groups of compounds in the oil. The information acquired could be useful for the selection of the most suitable moment in the ontogeny of the olive fruit for the search for key genes in the biosynthesis of phenolic compounds.
Research on the Multi-Angle Seismic Spectrum Response Law of Auxiliary Feedwater Electric Pumps
Authors: Tian Zhongxu , Chen Chang , Yuan Junting , Li Genpei
Published: December 2023

The auxiliary feedwater electric pump, which is vital in nuclear power units, demands exceptional seismic reliability. The impact of the seismic excitation direction on the seismic response of the complex auxiliary feedwater electric pump structure remains inadequately understood, thereby introducing uncertainties into seismic analysis and equipment installation procedures. To address this challenge, this study centers on a representative horizontal multi-stage centrifugal pump based on the finite element model and modal analysis. Utilizing the response spectrum method and the square root of the sum of the squares (SRSS) vibration pattern superposition principle, the research comprehensively explores the seismic response characteristics of individual pressure-bearing components and key rotor positions under seismic excitation from various angles. Findings reveal intricate variations in maximum stress and displacement responses for each pressure-bearing component under safe shutdown earthquake (SSE) level seismic excitation, corresponding to different input angles of seismic spectra. In assessing the seismic operability of the clearance between the impeller and the stator, the direction near 45° exhibits the maximum displacement response, highlighting the need for focused attention in testing and verification checks. The methodologies and conclusions presented in this paper offer valuable insights for designing, optimizing, and installing horizontal multi-stage centrifugal pumps, including auxiliary feedwater electric pumps, providing valuable guidance for future applications in the field.
The Electromagnetic Exposure Level of a Pure Electric Vehicle Inverter Based on a Real Human Body
Authors: Dong Xuwei , Gao Yunshan , Lu Mai
Published: December 2023

In order to quantitatively analyze the electromagnetic exposure dose of an inverter in a pure electric vehicle to the driver’s body and assess the safety of the electromagnetic exposure, based on a real human anatomy model in the virtual home project, a real human model with several organs and tissues, including muscles, bones, a heart, lungs, a liver, kidneys, a bladder, a skull, a scalp, white matter, and a cerebellum, was constructed. The inverter of a pure electric vehicle is considered to be the electromagnetic exposure source; for this study, an equivalent electromagnetic environment model composed of a real human body, an inverter, and a vehicle body was built. The distribution of induced fields in the driver’s tissues and organs was calculated and analyzed using the finite element method. The results show that the distribution of the magnetic flux density, induced electric field, and induced current density in the driver’s body was affected by the spatial distance of the inverter. The farther the distance was, the weaker the value was. Specifically, due to the different dielectric properties of the different tissues, the induced field in the different tissues was significantly different. However, the maximum magnetic flux density over the space occupied by the driver’s body and induced electric field in the driver’s trunk and central nervous system satisfied the exposure limits of the International Commission on Non-Ionization Radiation Protection, indicating that the electromagnetic environments generated by the inverter proposed in this paper are safe for the vehicle driver’s health. The numerical results of this study could also effectively supplement the study of the electromagnetic environments of pure electric vehicles and provide some references for protecting the drivers of pure electric vehicles from electromagnetic radiation.
Experimental Study on the Desalination of Waste Leachate Using the Combined Freezing Method
Authors: Wang Xiaokai , Zhao Changsheng , Guo Beibei , Zhang Bowei , Liu Xuzhen , Guo Yanke , Dong Yanan
Published: December 2023

The current high-salinity wastewater treatment technology is complex, costly, and carries the risk of secondary contamination. As a traditional desalination technology, the combined method using frozen technology has broad development prospects in wastewater treatment. This study investigates the desalination effects of waste leachate using three different methods: the frozen–gravity method (FGM), frozen–centrifugal method (FCM), and frozen–blowing methods (FBMs), under various experimental conditions. The results showed that the salt rejection of all three methods could reach more than 75% under the conditions of a freezing time of 12 h, freezing temperature of −15 °C, and ice production rate of 40%; the salt rejection of FGM increased at higher ambient temperatures, but it was not conducive to the removal of organic pollutants; the salt rejection of FCM was sensitive to the centrifugal time and centrifugal speed, with a significant correlation (p < 0.05), the increase in centrifugal time and centrifugal speed can help to improve the salt rejection, and the increase in centrifugal speed in the range of 1000–2000 rpm can accelerate the discharge of concentrated brine more effectively; the frozen–crushed–blowing method (FCBM) in FBM has a salt rejection as high as 93.86% at an ice production rate of 25.80%, which reduces the salinity of the effluent from 4.07% to 0.25%, speeds up the desalination process, and improves the salt rejection compared to the other methods. This study provides a new perspective and reference for the treatment of high-saline wastewater.
An Optimized Hardware Implementation of a Non-Adjacent Form Algorithm Using Radix-4 Multiplier for Binary Edwards Curves
Authors: Sajid Asher , Sonbul Omar S. , Rashid Muhammad , Arif Muhammad , Jaffar Amar Y.
Published: December 2023

Binary Edwards Curves (BEC) play a pivotal role in modern cryptographic processes and applications, offering a combination of robust security as well as computational efficiency. For robust security, this article harnesses the inherent strengths of BEC for the cryptographic point multiplication process by utilizing the Non-Adjacent Form (NAF) algorithm. For computational efficiency, a hardware architecture for the NAF algorithm is proposed. Central to this architecture is an Arithmetic Logic Unit (ALU) designed for streamlined execution of essential operations, including addition, squaring, and multiplication. One notable innovation in our ALU design is the integration of multiplexers, which maximize ALU efficiency with minimal additional hardware requirements. Complementing the optimized ALU, the proposed architecture incorporates a radix-4 multiplier, renowned for its efficiency in both multiplication and reduction. It eliminates resource-intensive divisions, resulting in a substantial boost to overall computational speed. The architecture is implemented on Xilinx Virtex series Field-Programmable Gate Arrays (FPGAs). It achieves throughput-to-area ratios of 14.819 (Virtex-4), 25.5 (Virtex-5), 34.58 (Virtex-6), and 37.07 (Virtex-7). These outcomes underscore the efficacy of our optimizations, emphasizing an equilibrium between computational performance and area utilization.
Integration of Organic Waste for Soil Stabilization through MICP
Authors: Golovkina Darya A. , Zhurishkina Elena V. , Filippova Arina D. , Baranchikov Alexander E. , Lapina Irina M. , Kulminskaya Anna A.
Published: December 2023

Microbial-induced calcite precipitation (MICP) is an innovative technology in civil engineering. However, the high cost of components and the fragility of the treated soil limit its wide use. One of the possible solutions is organic waste incorporation at different stages of the technology. In the present study, we consider the use of spent brewer’s yeast (BSY) to produce bacterial inoculates and wastepaper, flax shives and sawdust as reinforcing additives into the soil. We showed that the replacement of expensive components of LB medium by BSY extract increased biomass growth characteristics of Bacillus subtilis K51, B. cereus 4b and Micrococcus luteus 6 strains by 1.4, 1.5 and 1.8 times, respectively, while for B. subtilis 168, they were comparable to LB medium. The urease activities of all strains were not reduced compared to the control. Among the three kinds of cellulose-containing waste, wastepaper incorporation into MICP-treated soil samples led to an increase in compressive strength by 2.1 times and precipitated calcite percentage by almost 1.5 times compared to a sample without additives. Thus, we showed the potential for soil stabilization through MICP using organic waste.
Seismic Resilience Evolution of Shield Tunnel with Structure Degradation
Authors: Cui Hongzhi , Tao Ran , Bao Xiaohua , Wu Xianlong , Qiu Tong , Shen Jun , Han Zhen , Chen Xiangsheng
Published: December 2023

The structural performance of shield tunnel structures is highly susceptible to degradation under complex environmental loads, with the most common manifestation being bolt preload loss. In this study, a shield tunnel numerical simulation model was established to analyze the seismic response of shield tunnels with varying degrees of bolt preload loss. Firstly, the deformation patterns of shield tunnel structures under seismic loads were analyzed. Subsequently, ellipticity and joint opening were selected as seismic resilience assessment indicators based on the mechanical response. A seismic resilience assessment model was then established, including three states: normal state, affected state, and recovered state. The results show a direct relationship between the recovery capacity of tunnel structures and the initial performance of the lining structure, as well as the magnitude of the load. The lower the degree of structure degradation, the greater the structural recovery capacity. Additionally, there is a positive correlation between residual deformation and the initial performance loss of shield tunnel structures, as well as the intensity of seismic loads. This study contributes to enriching the theoretical framework for the seismic resilience assessment of shield tunnels, which have significant implications and provide valuable references for engineering safety.
Switch-Independent 3A: An Epigenetic Regulator in Cancer with New Implications for Pulmonary Arterial Hypertension
Authors: Jankowski Katherine , Jagana Vineeta , Bisserier Malik , Hadri Lahouaria
Published: December 2023

Epigenetic mechanisms, including DNA methylation, histone modifications, and non-coding RNA, play a crucial role in the regulation of gene expression and are pivotal in biological processes like apoptosis, cell proliferation, and differentiation. SIN3a serves as a scaffold protein and facilitates interactions with transcriptional epigenetic partners and specific DNA-binding transcription factors to modulate gene expression by adding or removing epigenetic marks. However, the activation or repression of gene expression depends on the factors that interact with SIN3a, as it can recruit both transcriptional activators and repressors. The role of SIN3a has been extensively investigated in the context of cancer, including melanoma, lung, and breast cancer. Our group is interested in defining the roles of SIN3a and its partners in pulmonary vascular disease. Pulmonary arterial hypertension (PAH) is a multifactorial disease often described as a cancer-like disease and characterized by disrupted cellular metabolism, sustained vascular cell proliferation, and resistance to apoptosis. Molecularly, PAH shares many common signaling pathways with cancer cells, offering the opportunity to further consider therapeutic strategies used for cancer. As a result, many signaling pathways observed in cancer were studied in PAH and have encouraged new research studying SIN3a’s role in PAH due to its impact on cancer growth. This comparison offers new therapeutic options. In this review, we delineate the SIN3a-associated epigenetic mechanisms in cancer and PAH cells and highlight their impact on cell survival and proliferation. Furthermore, we explore in detail the role of SIN3a in cancer to provide new insights into its emerging role in PAH pathogenesis.
Redox-Active Cerium Fluoride Nanoparticles Selectively Modulate Cellular Response against X-ray Irradiation In Vitro
Authors: Chukavin Nikita N. , Filippova Kristina O. , Ermakov Artem M. , Karmanova Ekaterina E. , Popova Nelli R. , Anikina Viktoriia A. , Ivanova Olga S. , Ivanov Vladimir K. , Popov Anton L.
Published: December 2023

Ionizing radiation-induced damage in cancer and normal cells leads to apoptosis and cell death, through the intracellular oxidative stress, DNA damage and disorders of their metabolism. Irradiation doses that do not lead to the death of tumor cells can result in the emergence of radioresistant clones of these cells due to the rearrangement of metabolism and the emergence of new mutations, including those in the genes responsible for DNA repair. The search for the substances capable of modulating the functioning of the tumor cell repair system is an urgent task. Here we analyzed the effect of cerium(III) fluoride nanoparticles (CeF3 NPs) on normal (human mesenchymal stem cells–hMSC) and cancer (MCF-7 line) human cells after X-ray radiation. CeF3 NPs effectively prevent the formation of hydrogen peroxide and hydroxyl radicals in an irradiated aqueous solution, showing pronounced antioxidant properties. CeF3 NPs are able to protect hMSC from radiation-induced proliferation arrest, increasing their viability and mitochondrial membrane potential, and, conversely, inducing the cell death of MCF-7 cancer cells, causing radiation-induced mitochondrial hyperpolarization. CeF3 NPs provided a significant decrease in the number of double-strand breaks (DSBs) in hMSC, while in MCF-7 cells the number of γ-H2AX foci dramatically increased in the presence of CeF3 4 h after irradiation. In the presence of CeF3 NPs, there was a tendency to modulate the expression of most analyzed genes associated with the development of intracellular oxidative stress, cell redox status and the DNA-repair system after X-ray irradiation. Cerium-containing nanoparticles are capable of providing selective protection of hMSC from radiation-induced injuries and are considered as a platform for the development of promising clinical radioprotectors.
Changes in Cognitive Functions after Carotid Endarterectomy and Carotid Stenting: A Decade-Apart Comparison
Authors: Václavík Daniel , Pakizer David , Hrbáč Tomáš , Roubec Martin , Procházka Václav , Jonszta Tomáš , Herzig Roman , Školoudík David
Published: December 2023

Background: This study investigates changes in cognitive function in patients with severe carotid stenosis who underwent carotid endarterectomy (CEA) and carotid stenting (CAS) over two decades. Methods: We compared cognitive function within 30 days after the procedure in 267 patients (first 100 each for CEA and CAS in two periods: 2008–2012 and 2018–2022) in a single institution. Assessments used Adenbrooke’s Cognitive Examination–Revised (ACE-R), the Mini-Mental State Examination (MMSE), Speech Fluency Test (SFT), and Clock Drawing Test (CDT), conducted before and 30 ± 2 days after surgery. Results: Patients (mean age 67.2 years, 70%+ carotid stenosis) exhibited different cognitive changes over periods. In 2008–2012, significant declines in MMSE (CEA, p = 0.049) and CDT (CAS, p = 0.015) were observed among asymptomatic patients. On the contrary, in 2018–2022, improvements were observed in ACE-R and MMSE for symptomatic and asymptomatic patients undergoing CEA and CAS. Conclusion: Over a decade, advances in interventional techniques and patient management have reduced risks of cognitive decline in patients with asymptomatic carotid stenosis and also have improved cognitive functions in both symptomatic and asymptomatic individuals.
The Dynamic SUMOylation Changes and Their Potential Role in the Senescence of APOE4 Mice
Authors: Xu Yangqi , Cai Wenwen , Sang Shaoming , Cheng Xiaoqin , Jin Boru , Zhao Xiangteng , Zhong Chunjiu
Published: December 2023

The ε4 allele of apolipoprotein E (APOE4) and aging are the major risk factors for Alzheimer’s disease (AD). SUMOylation is intimately linked to the development of AD and the aging process. However, the SUMOylation status in APOE4 mice has not been uncovered. In this study, we investigated SENP1 and SUMOylation changes in the brains of aged APOE3 and APOE4 mice, aiming to understand their potential impact on mitochondrial metabolism and their contribution to cellular senescence in APOE4 mice. Concurrently, SUMO1-conjugated protein levels decreased, while SUMO2/3-conjugated protein levels increased relatively with the aging of APOE4 mice. This suggests that the equilibrium between the SUMOylation and deSUMOylation processes may be associated with senescence and longevity. Our findings highlight the significant roles of SENP1 and SUMOylation changes in APOE4-driven pathology and the aging process.
Global Neuropsychopharmacological Prescription Trends in Adults with Schizophrenia, Clinical Correlates and Implications for Practice: A Scoping Review
Authors: Ying Jiangbo , Chew Qian Hui , Wang Yuxi , Sim Kang
Published: December 2023

It is important to examine the psychotropic prescription practices in schizophrenia, as it can inform regarding changing treatment choices and related patient profiles. No recent reviews have evaluated the global neuropsychopharmacological prescription patterns in adults with schizophrenia. A systematic search of the literature published from 2002 to 2023 found 88 empirical papers pertinent to the utilization of psychotropic agents. Globally, there were wide inter-country and inter-regional variations in the prescription of psychotropic agents. Overall, over time there was an absolute increase in the prescription rate of second-generation antipsychotics (up to 50%), mood stabilizers (up to 15%), and antidepressants (up to 17%), with an observed absolute decrease in the rate of antipsychotic polypharmacy (up to 15%), use of high dose antipsychotic (up to 12% in Asia), clozapine (up to 9%) and antipsychotic long-acting injectables (up to 10%). Prescription patterns were mainly associated with specific socio-demographic (such as age), illness (such as illness duration), and treatment factors (such as adherence). Further work, including more evidence in adjunctive neuropsychopharmacological treatments, pharmaco-economic considerations, and examination of cohorts in prospective studies, can proffer insights into changing prescription trends relevant to different treatment settings and predictors of such trends for enhancement of clinical management in schizophrenia.
The Adaptability of Residential Planning and Design to World-Changing Events
Authors: Duarte Carlos C. , Cortiços Nuno D. , Stefańska Anna , Mateus Daniel , Monticelli Carol
Published: December 2023

History has witnessed several events with different magnitudes that have served as catalysts for drastic changes in society, impacting people’s lifestyles and reshaping the prevailing civilizational paradigm [...]
A Systematic Review of Climate Change Implications on Building Energy Consumption: Impacts and Adaptation Measures in Hot Urban Desert Climates
Authors: Kutty Najeeba Abdulla , Barakat Dua , Darsaleh Abeer Othman , Kim Young Ki
Published: December 2023

The climate change–built environment nexus is complex and intertwined. Recognizing the rising air temperatures and solar radiations owing to climate-induced global warming, it is critical to manage the increased building energy and cooling loads in the Middle East Gulf states’ hot desert climates (Bwh). One of the top climate priorities is to promote climate resilience by reducing risks and enhancing adaptation options. This study aims to systematically review the existing literature to document building energy performances in and the associated adaptation measures of the Middle East Gulf states, regarding the implications of climate change. It is accomplished by answering the following questions: ‘How well do we understand the effects of climate change on building energy use in hot urban deserts?’ and ‘What are the most appropriate adaptation strategies to reduce energy use in hot urban deserts?’. Using the Preferred Reporting Items for Systematic review and Meta-Analysis protocols (PRISMA), 17 studies on the influence of present and future weather scenarios on building performance are examined, considering variations in typology, methods, and input variables. Finally, the paper identifies the preferred methods and input variables for modelling building energy performance under predicted climatic changes. Passive design considerations are considered highly effective in mitigating and adapting to climate change implications. Thermal insulation and efficient window glazing are identified as the best-performing strategies, while the use of solar Photovoltaic (PV) is considered efficient in meeting the primary energy demands. The study’s findings can assist planners and designers in projecting future climatic influences on the energy usage of existing buildings.
Nitrogen-Rich Tetrazole–Amide-Functionalised Zn Metal–Organic Framework as Catalyst for Efficient Catalytic CO2 Cycloaddition with Epoxides
Authors: Paul Anup , Garazade Ismayil M. , Karmakar Anirban , Khan Rais Ahmad , Guedes da Silva Maria Fátima C. , Nunes Ana V. M. , Pombeiro Armando J. L.
Published: December 2023

In this study, we report the design, synthesis, and catalytic application of the novel nitrogen-rich Zn(II) MOF [Zn2(μ3-1κN,2κN′,3κO-HL)2(DMF)2]n·nH2O (HL2− = 4-((4-(1H-tetrazol-5-yl)phenyl)carbamoyl)benzoate), denoted as ZnMOF, for the efficient conversion of carbon dioxide (CO2) to cyclic carbonates via cycloaddition with epoxides. It was synthesised from a tetrazole appended amide-functionalised pro-ligand (H3L) and Zn(NO3)2·6H2O under hydrothermal conditions. The synthesised material was characterised namely by elemental analysis, infrared spectroscopy, powder X-ray diffraction (PXRD), and single-crystal X-ray diffraction analyses. The catalytic potential of ZnMOF was investigated in the CO2 cycloaddition reaction with various epoxides, with conversions ranging from 17% to 99%. The catalyst retained its activity across multiple reaction cycles, demonstrating its stability and reusability. The influence of co-catalysts on the reaction was explored, with tetrabutylammonium bromide (TBABr) emerging as the most effective one. A plausible reaction mechanism is proposed.
Density Functional Theory Study of CuAg Bimetal Electrocatalyst for CO2RR to Produce CH3OH
Authors: Xue Sensen , Liang Xingyou , Zhang Qing , Ren Xuefeng , Gao Liguo , Ma Tingli , Liu Anmin
Published: December 2023

Converting superfluous CO2 into value-added chemicals is regarded as a practical approach for alleviating the global warming problem. Powered by renewable electricity, CO2 reduction reactions (CO2RR) have attracted intense interest owing to their favorable efficiency. Metal catalysts exhibit high catalytic efficiency for CO2 reduction. However, the reaction mechanisms have yet to be investigated. In this study, CO2RR to CH3OH catalyzed by CuAg bimetal is theoretically investigated. The configurations and stability of the catalysts and the reaction pathway are studied. The results unveil the mechanisms of the catalysis process and prove the feasibility of CuAg clusters as efficient CO2RR catalysts, serving as guidance for further experimental exploration. This study provides guidance and a reference for future work in the design of mixed-metal catalysts with high CO2RR performance.
Ultrafiltration to Increase the Consistency of Fruit Pulps: The Role of Permeate Flux
Authors: Chiampo Fulvia
Published: December 2023

Ultrafiltration is a well-known operation, widely used in food processing, especially to concentrate selectively liquid compounds. However, so far, it has been mainly used to change concentration and/or clarify liquids with low viscosity. Ultrafiltration has seldomly been applied to viscous fluids. In this study, it was used to increase the consistency of fruit pulps, without changing their taste and organoleptic properties. This paper reports the findings achieved in experimental runs carried out on a pilot plant, equipped with four ultrafiltration tubular membranes (total surface area = 0.8 m2). Raw fruit pulps, namely, apple, apricot, and pear, were used to study the influence of the operative parameters on the permeate flux and organoleptic properties of the final products (retentate and permeate). The flow rate was in the range of 3.0–5.1 m3/h, at 50 °C. The influence of temperature on the permeate flux was checked, with one run with apple pulp at 20 °C. As expected, the findings show that high flow rate and temperature improve the permeate flux. Membranes show different performance in permeate flux for the tested pulps. This is probably due to their different chemical and physical composition, which could be responsible for different fouling of the membrane and, as a consequence, a different resistance to the permeate flow. The final products have the same taste as the raw ones, and each of them can be used as it is or as an ingredient. These results have a technological relevance, and, besides, the study shows a methodology for future applications of ultrafiltration.
Excess BAFF May Impact HIV-1-Specific Antibodies and May Promote Polyclonal Responses Including Those from First-Line Marginal Zone B-Cell Populations
Authors: Doyon-Laliberté Kim , Aranguren Matheus , Chagnon-Choquet Josiane , Batraville Laurie-Anne , Dagher Olina , Richard Jonathan , Paniconi Matteo , Routy Jean-Pierre , Tremblay Cécile , Quintal Marie-Claude , Brassard Nathalie , Kaufmann Daniel E. , Finzi Andrés , Poudrier Johanne , Roger Michel
Published: December 2023

We have previously shown that blood levels of B-cell Activating Factor (BAFF) rise relatively to disease progression status in the context of HIV-1 infection. Excess BAFF was concomitant with hyperglobulinemia and the deregulation of blood B-cell populations, notably with increased frequencies of a population sharing characteristics of transitional immature and marginal zone (MZ) B-cells, which we defined as marginal zone precursor-like” (MZp). In HIV-uninfected individuals, MZp present a B-cell regulatory (Breg) profile and function, which are lost in classic-progressors. Moreover, RNASeq analyses of blood MZp from classic-progressors depict a hyperactive state and signs of exhaustion, as well as an interferon signature similar to that observed in autoimmune disorders such as Systemic Lupus Erythematosus (SLE) and Sjögren Syndrome (SS), in which excess BAFF and deregulated MZ populations have also been documented. Based on the above, we hypothesize that excess BAFF may preclude the generation of HIV-1-specific IgG responses and drive polyclonal responses, including those from MZ populations, endowed with polyreactivity/autoreactivity. As such, we show that the quantity of HIV-1-specific IgG varies with disease progression status. In vitro, excess BAFF promotes polyclonal IgM and IgG responses, including those from MZp. RNASeq analyses reveal that blood MZp from classic-progressors are prone to Ig production and preferentially make usage of IGHV genes associated with some HIV broadly neutralizing antibodies (bNAbs), but also with autoantibodies, and whose impact in the battle against HIV-1 has yet to be determined.
Using the Essential Oils of Sage and Anise to Enhance the Shelf Life of the Williams (sin. Bartlett) Pear
Authors: Ionica Mira Elena , Bita Andrei , Tutulescu Felicia
Published: December 2023

The effects of post-harvest spray treatments with essential oils (EOs) obtained from sage and aniseed on maintaining the quality of pears of the ‘Williams’ variety during storage was studied. Harvested pears were picked when they had reached their optimal maturity and underwent a treatment involving the application of aqueous solutions of glycerin, with varying amounts of sage essential oil (SEO) and aniseed essential oil (AEO). Weight loss during storage varied according to the treatment applied with the lowest values recorded for sage essential oil at concentrations of 300 ppm (6.24%) and 250 ppm (6.60%), respectively. Aniseed essential oil had a smaller effect on weight loss compared to sage essential oil. Fruit firmness was better maintained under the influence of the essential oil treatments, with those treated with sage essential oil standing out. The concentration of the essential oils that is used influences the antimicrobial activity of the post-harvest treatment that is applied, with higher essential oil concentrations leading to more pronounced decreases in the total number of mesophilic aerobic bacteria immediately after treatment (4.05 for SEO 200; 3.00 for SEO 300, respectively). The use of post-harvest techniques involving the application of aqueous solutions containing glycerol and essential plant oils by spraying can extend the shelf life of pear fruits.
Performance and Application Analysis of a New Optimization Algorithm
Authors: Zheng Junlong , Jettanasen Chaiyan , Chiradeja Pathomthat
Published: December 2023

Our research focused on an optimization algorithm. Our work makes three contributions. First, a new optimization algorithm, the Maritime Search and Rescue Algorithm (MSRA), is creatively proposed. The algorithm not only has better optimization performance, but also has the ability to plan the path to the best site. For other existing intelligent optimization algorithms, it has never been found that they have both of these performances. Second, the mathematical model of the MSRA was established, and the computer program pseudo-code was created. Third, the MSRA was verified by experiments.
SPHYNCS: The Use of the Swiss Narcolepsy Scale in a New Cohort of Patients with Narcolepsy and Its Borderland and Review of the Literature
Authors: Zub Kseniia , Warncke Jan D. , van der Meer Julia , Wenz Elena S. , Fregolente Livia G. , Bargiotas Panagiotis , Benbir Şenel Gülçin , Sturzenegger Christian , Lammers Gert Jan , Kallweit Ulf , Schmidt Markus H. , Khatami Ramin , Zhang Zhongxing , von Manitius Sigrid , Miano Silvia , Acker Jens G. , Strub Matthias , Bassetti Claudio L. A.
Published: December 2023

Introduction and aims: Narcolepsy type 1 (NT1) is a central disorder of hypersomnolence (CDH) characterized by excessive daytime sleepiness and cataplexy. The Swiss Narcolepsy Scale (SNS), which includes the updated and short (sSNS) versions, has recently been introduced as a reliable diagnostic tool for identifying NT1. This study aims to assess the validity of the SNS scales in a new cohort of patients with CDH, while also introducing the French and Italian versions of the SNS and providing a summary of the existing literature on SNS. Materials and methods: The current study is based on the international Swiss Primary Hypersomnolence and Narcolepsy Cohort Study (iSPHYNCS) which aims to identify new biomarkers for CDH. Diagnostic accuracy of the SNS was assessed by calculating sensitivity, specificity, positive predictive value, and negative predictive value. Results: In our population, 108 participants with suspected CDH (including 28 NT1 patients) and 14 healthy controls completed the scale. Original SNS, updated SNS and sSNS scores showed a high sensitivity (86%, 89% and 79%, respectively) and high specificity (96%, 90% and 95%, respectively) for diagnosing NT1 compared to other CDH. The French version was completed by 5 participants, and the Italian version by 8 participants. Regarding previous studies, the SNS has now been assessed in six different populations, involving a total of 1247 subjects (including 326 with narcolepsy with cataplexy/NT1), suggesting high sensitivity (85–100%) and specificity (86–100%) of the SNS for the diagnosis of NT1. Conclusion: The SNS is a simple screening tool validated in seven languages (German, English, French, Italian, Dutch, Turkish and Japanese), demonstrating high sensitivity and specificity for the diagnosis of NT1.
Estimating Local Diagnostic Reference Levels for Mammography in Dubai
Authors: Abdulwahid Noor Kaltham , Mohd Norsuddin Norhashimah , Abdul Karim Muhammad Khalis , Che Isa Iza Nurzawani , Alshamsi Wadha
Published: December 2023

As the total volume of mammograms in Dubai is increasing consistently, it is crucial to focus on the process of dose optimization by determining dose reference levels for such sensitive radiographic examinations as mammography. This work aimed to determine local diagnostic reference levels (DRLs) for mammography procedures in Dubai at different ranges of breast thickness. A total of 2599 anonymized mammograms were randomly retrieved from a central dose survey database. Mammographic cases for screening women aged from 40 to 69 years were included, while cases of breast implants and breast thickness outside the range of 20–100 mm were excluded. Mean, median, and 75 percentiles were obtained for the mean glandular dose (MGD) distribution of each mammography projection for all compressed breast thickness (CBT) ranges. The local DRLs for mammography in Dubai were found to be between 0.80 mGy and 0.82 mGy for the craniocaudal (CC) projection and between 0.89 mGy and 0.971.8 mGy for the mediolateral oblique (MLO) projection. Local DRLs were proposed according to different breast thicknesses, starting from 20 to 100 mm. All groups of CBT showed a slight difference in MGD values, with higher values in MLO views rather than CC views. The local DRLs in this study were lower than some other Middle Eastern countries and lower than the standard reference levels reported by the International Atomic Energy Agency (IAEA) at 3 mGy/view.
Advancements in Endoscopic Resection for Colitis-Associated Colorectal Neoplasia in Inflammatory Bowel Disease: Turning Visible into Resectable
Authors: Maselli Roberta , de Sire Roberto , Massimi Davide , Franchellucci Gianluca , Busacca Anita , Castiglione Fabiana , Rispo Antonio , Hassan Cesare , Armuzzi Alessandro , Repici Alessandro
Published: December 2023

Patients suffering from inflammatory bowel disease (IBD) face a two to three-fold higher risk of developing colorectal cancer (CRC) compared to the general population. In recent years, significant progress has been made in comprehending the natural history of IBD-associated CRC (IBD-CRC) and refining its treatment strategies. The decreased incidence of IBD-CRC can be attributed to improved therapeutic management of inflammation, advancements in endoscopy, and early detection of precancerous lesions via surveillance programs. Advanced imaging technologies have made previously undetectable dysplasia visible in most cases, allowing for a much more precise and detailed examination of the mucosa. Additionally, new tools have facilitated the endoscopic resection (ER) of visible lesions in IBD. Particularly, the key to effectively manage colitis-associated colorectal neoplasia (CAN) is to first identify it and subsequently guarantee a complete ER in order to avoid surgery and opt for continuing surveillance. Advanced ER techniques for CAN include endoscopic mucosal resection (EMR), endoscopic submucosal dissection (ESD), and hybrid ESD-EMR (h-ESD). This narrative review aims to consolidate the current literature on IBD-CRC, providing an overview of advanced techniques for ER of CAN in IBD, with a particular emphasis on the impact of ESD on the long-term outcomes of IBD patients.
Fascial Signal Change on the Cervical MRI of a Patient with Systemic Lupus Erythematosus
Authors: Kim Hyun-Je , Lee Dong Gyu
Published: December 2023

Here, we present a case of a 53-year-old female patient with chronic neck pain and systemic inflammation who was ultimately diagnosed with systemic lupus erythematosus. Notably, applying fat-suppressed T2-weighted MRI sequences was pivotal in detecting structural fascial changes commonly associated with systemic inflammatory diseases. PET-CT scans further revealed systemic inflammation around multiple joints, providing valuable insights into MRI signal alterations. This case underscores the importance of considering systemic autoimmune pathology as a potential underlying cause of chronic musculoskeletal pain. It also highlights the clinical utility of MRI with fat suppression sequences in identifying inflammation-related fascial changes. This case emphasizes the significance of a comprehensive evaluation, particularly in situations where clinical features overlap between autoimmune and degenerative skeletal pathologies. Fat-suppressed MRI can provide information about fascial pathology related to systemic inflammatory diseases. In this context, it is worth noting that PET-CT and fat suppression MRI complement each other by providing complementary information about inflammation and the underlying causes of a patient’s pain.
Exploring the Role of Intraoperative Positive Culture of Allograft Bone in Subsequent Postoperative Infections among Donors and Recipients in Bone Bank Processing
Authors: Tsai Yao-Hung , Chen Hung-Yen , Huang Tsung-Yu , Chen Jiun-Liang , Kuo Liang-Tseng , Huang Kuo-Chin
Published: December 2023

Background: Allografts have been frequently used in orthopedic procedures. The purposes of this study were to evaluate the discard rates and bacterial contamination of a bone bank, and to assess the clinical outcomes of recipients with bacterial culture-positive donor allografts. Methods: We retrospectively reviewed 1764 allografts which were harvested from living donors and stored in a bone bank from 2018 to 2022. The donors whose allografts displayed bacterial contamination at retrieval of the primary hip or knee arthroplasty were followed for microbiology and subsequent prosthetic joint infection analysis. The infected pathogens, antibiotic treatment and subsequent infection were reviewed for the intraoperative positive culture group. Results: The discard rate was 17%, and the bacterial contamination rate of bone retrieval was 2.15%. Thirty-eight allografts at retrieval displayed confirmed bacterial growth, and 37 patients did not reveal infective signs at 6 months follow-up. A total of 1464 allografts were stored and implanted, among which 28 allografts (1.91%) were confirmed to be positive for bacterial growth and 13 cases (0.89%) were confirmed as surgical site infections. Conclusions: Our results validate the suggestion that our bone bank system performs good quality monitoring to eliminate the risk of dissemination of viral and bacterial diseases and to decrease surgical site infection after allograft implantation. By ensuring aseptic conditions and contamination-reducing strategies during harvesting and thawing, the allografts can be safely stored and implanted while limiting bacterial contamination. Our findings confirm that the intraoperative positive cultures of allografts did not contribute to subsequent postoperative surgical site infection in donors and recipients.