bluuebunny/crossref_metadata_2025 huggingface dataset:
Respiratory Health among Pesticide Sprayers at Flower Farms in Ethiopia
Authors: Shentema Meaza Gezu , Bråtveit Magne , Kumie Abera , Deressa Wakgari , Moen Bente Elisabeth
Published: June 2022

Background: Pesticide use in Ethiopia has become a common practice in which large-scale flower farms are the main consumers. Workers on flower farms might be exposed to pesticides while spraying or while performing other tasks related to pesticide use and management. It is unclear whether working as a flower farm sprayer is associated with respiratory health problems. Objective: The objective of this study was to compare respiratory symptoms and lung function indices between pesticide sprayers and non-spraying workers. Method: A cross-sectional study was conducted on 15 flower farms, involving all-male sprayers as the pesticide-exposed group and all other male workers as a control group. Data were collected using a standard questionnaire for respiratory symptoms developed by the British Medical Research Council and the American Thoracic Society. Lung function tests were performed to determine forced vital capacity (FVC), forced expiratory volume at one second (FEV1), mid 50 expiratory flow, and the ratio of FEV1 to FVC. Chi-squared tests and Poisson regression analyses were used to compare respiratory symptoms between the two working groups. General linear regression models were used to compare lung function test indices between spraying and non-spraying working groups. The significance level was set to 0.05. Results: A total of 285 male workers participated (152 sprayers and 133 non-spraying workers). The mean age of the workers was 25 years for sprayers and 24 years for non-sprayers. The proportions of cough, cough with sputum, breathlessness, and wheezing were similar in the two groups, while chest tightness was significantly high in the non-spraying group. Sprayers had significantly higher FVC and FEV1 than the non-spraying group. Conclusions: Respiratory symptoms were not different between the sprayers and non-spraying workers except that the non-spraying workers had increased chest tightness. FVC and FEV1 were significantly higher among sprayers relative to non-sprayers. The results must be interpreted with caution, as the sprayers used respiratory protective equipment, which probably reduced their exposure to the pesticides. Also, the workers were young, and a healthy worker effect might be present among the sprayers.
Transcription Factor AhR, Cytokines IL-6 and IL-22 in Subjects with and without Peri-Implantitis: A Case Control-Study
Authors: Martins Luis Ricardo Linard , Grzech-Leśniak Kinga , Castro dos Santos Nidia , Suárez Lina J. , Giro Gabriela , Bastos Marta Ferreira , Shibli Jamil Awad
Published: June 2022

Peri-implantitis is a plaque-associated condition characterized by mucosal inflammation and subsequent progressive loss of supporting bone; it is caused by bacterial biofilm, but the host response triggered by bacterial stimulation promotes the release of cells and mediators that culminate in tissue destruction. The Aryl-hydrocarbon Receptor (AhR) is associated with IL-22 production by Th22 and Th17 CD4+ Th cells. The presence of IL-6 may promote the Th22 phenotype. The present case-control study evaluated the gene expression of AhR, IL-22, and IL-6 in the peri-implant tissues of healthy and peri-implantitis patients. Tissue biopsies were collected from thirty-five volunteers (15 healthy and 20 with peri-implantitis). A real-time PCR reaction was utilized to assess the AhR, IL-22, and IL-6 gene expression levels relative to the reference gene (GAPDH). The results were analyzed using the Mann–Whitney test with a significance level of 5%. Higher levels of gene expression of AhR and IL-6 were detected in peri-implantitis tissues. The IL-22 gene expression levels did not differ between groups. In conclusion, higher gene expression levels for AhR and IL-6 were detected in the soft tissues of peri-implantitis patients. IL-22 did not vary between conditions, which may indicate the loss of the immunomodulatory role of IL-22 in periimplantitis.
Is Teleaudiology Achieving Person-Centered Care: A Review
Authors: Brice Sophie , Almond Helen
Published: June 2022

Digital health and person-centered care are unquestionably linked in today’s Australian healthcare landscape. Teleaudiology is the application of digital health in the field of audiology, and it has become a popular component of standard audiological care. Behavior modification is essential in audiology intervention. Guidance on achieving behavior change, which is dependent on digitally enabled intervention, is a valuable resource when used in tandem to achieve person-centered care. The aim of this review is to determine whether teleaudiology achieves person-centered care. A qualitative review was conducted, followed by mapping and analysis. Analysis identified evidence of teleaudiology use, and ascertained guiding principles are appropriate to behavior change dependent digital intervention supported or enabled person-centered care. In conclusion, teleaudiology will continue to be a promising technology for promoting relatedness, a positive user experience, confidence and capability, and appropriate levels of autonomy for the user to choose from among the person-centered care options available.
Understanding Impacts of SNAP Fruit and Vegetable Incentive Program at Farmers’ Markets: Findings from a 13 State RCT
Authors: Karpyn Allison , Pon Julia , Grajeda Sara B. , Wang Rui , Merritt Kathryn E. , Tracy Tara , May Henry , Sawyer-Morris Ginnie , Halverson McKenna M. , Hunt Alan
Published: June 2022

Disparities in healthy food access and consumption are a major public health concern. This study reports the findings from a two-year randomized control trial conducted at 77 farmers’ markets (FMs) in 13 states and the District of Columbia that sought to understand the impact of fruit and vegetable (FV) incentive vouchers, randomly issued at varied incentive levels to Supplemental Nutrition Assistance Program (SNAP) recipients, for use at FMs. Measures included FV and overall household food purchasing; FV consumption; food insecurity; health status; market expenditure; and demographics. A repeated-measures mixed-effects analysis and the Complier Average Causal Effect (CACE) were used to examine outcomes. Despite 82% reporting food insecurity in the prior year, the findings showed that financial incentives at FMs had statistically significant, positive effects on FV consumption; market expenditures increased with added incentives. SNAP recipients receiving an incentive of USD 0.40 for every USD 1.00 in SNAP spent an average of USD 19.03 per transaction, while those receiving USD 2 for every USD 1 (2:1) spent an average of USD 36.28 per transaction. The data showed that the incentive program at the highest level (2:1) maximally increased SNAP FM expenditure and FV consumption, increasing the latter by 0.31 daily cups among those who used their incentive (CACE model).
Problematic Smartphone Use Leads to Behavioral and Cognitive Self-Control Deficits
Authors: Fabio Rosa Angela , Stracuzzi Alessia , Lo Faro Riccardo
Published: June 2022

Excessive use of smartphones has been associated with a number of negative consequences for individuals. Some of these consequences relate to many symptoms of behavioral addiction. The present study aims to investigate whether participants with high levels of smartphone usage may have difficulty with their ability to wield the self-control that is needed to restrict smartphone usage compared to participants with lower levels of smartphone addiction. Specifically, we expect that people with high levels of smartphone usage may have problems in refraining from using a smartphone. In addition, we expect people with a high level of smartphone use may show deficiencies in cognitive tasks such as memory, executive control, and visual and auditory attention. An ABA design was applied to analyze the effects of smartphone withdrawal. The first A refers to baseline measurements: Visual RT, Auditory RT, Go/No-Go RT and N-Back RT and Eriksen flanker RT. The B refers to 3 days of smartphone withdrawal, whereas the second A refers to the same measurements used in the baseline. In addition, several standardized scales were administered, among them: Smartphone addiction scale-short version (SAS-SV), Fear of missing out scale (FoMOs), Procrastination scale, and Psychological General Well-Being Index. One hundred and eleven participants took part in the study. Based on median split they were divided into two groups: high level and low level smartphone users. Moreover, thanks to an app installed on the participants’ smartphones, it was possible to measure levels of compliance with the task. Results indicate that participants with low levels of smartphone usage show less difficulty in their ability to wield the self-control needed to withdraw smartphone use and faster reaction times on cognitive tests than participants with high levels of smartphone usage. Moreover, the profile of participants with high levels of smartphone usage shows higher scores on the FoMOs and Procrastination scale, and lower scores in the Psychological General Well-Being Index. The results are discussed in light of self-regulation theory.
Prevalence of Orthodontic Malocclusions in Healthy Children and Adolescents: A Systematic Review
Authors: De Ridder Lutgart , Aleksieva Antonia , Willems Guy , Declerck Dominique , Cadenas de Llano-Pérula Maria
Published: June 2022

The purpose of this study was to systematically review the literature regarding the prevalence of malocclusion and different orthodontic features in children and adolescents. Methods: The digital databases PubMed, Cochrane, Embase, Open Grey, and Web of Science were searched from inception to November 2021. Epidemiological studies, randomized controlled trials, clinical trials, and comparative studies involving subjects ≤ 18 years old and focusing on the prevalence of malocclusion and different orthodontic features were selected. Articles written in English, Dutch, French, German, Spanish, and Portuguese were included. Three authors independently assessed the eligibility, extracted the data from, and ascertained the quality of the studies. Since all of the included articles were non-randomized, the MINORS tool was used to score the risk of bias. Results: The initial electronic database search identified a total of 6775 articles. After the removal of duplicates, 4646 articles were screened using the title and abstract. A total of 415 full-text articles were assessed, and 123 articles were finally included for qualitative analysis. The range of prevalence of Angle Class I, Class II, and Class III malocclusion was very large, with a mean prevalence of 51.9% (SD 20.7), 23.8% (SD 14.6), and 6.5% (SD 6.5), respectively. As for the prevalence of overjet, reversed overjet, overbite, and open bite, no means were calculated due to the large variation in the definitions, measurements, methodologies, and cut-off points among the studies. The prevalence of anterior crossbite, posterior crossbite, and crossbite with functional shift were 7.8% (SD 6.5), 9.0% (SD 7.34), and 12.2% (SD 7.8), respectively. The prevalence of hypodontia and hyperdontia were reported to be 6.8% (SD 4.2) and 1.8% (SD 1.3), respectively. For impacted teeth, ectopic eruption, and transposition, means of 4.9% (SD 3.7), 5.4% (SD 3.8), and 0.5% (SD 0.5) were found, respectively. Conclusions: There is an urgent need to clearly define orthodontic features and malocclusion traits as well as to reach consensus on the protocols used to quantify them. The large variety in methodological approaches found in the literature makes the data regarding prevalence of malocclusion unreliable.
Voronoi Diagrams for Senior-Friendly Cities
Authors: Figurska Marta , Dawidowicz Agnieszka , Zysk Elżbieta
Published: June 2022

Motives: Active aging places (AAP) should be identified during the COVID-19 pandemic to ensure the sanitary safety of seniors, prevent older adults from feeling excluded, and eliminate health threats that discourage seniors from being active. Aim: The aim of this study was to apply a new analytical approach with the use of Voronoi diagrams in GIS tools to spatially identify the AAP in the context of apparent social dynamics. Methods: An empirical study was conducted with the use of qualitative (literature review, questionnaire survey, AAP classification, visualization of AAP location with GIS tools) and quantitative methods (AAP ranking based on a statistical analysis of survey responses). Results: Voronoi diagrams were used to determine the accessibility of selected objects in the city of Olsztyn and identify spaces that belong to the social logic of space.
Assessment of Physical Fitness and Risk Factors for the Occurrence of the Frailty Syndrome among Social Welfare Homes’ Residents over 60 Years of Age in Poland
Authors: Kaczorowska Antonina , Szwamel Katarzyna , Fortuna Małgorzata , Mroczek Agata , Lepsy Ewelina , Katan Aleksandra
Published: June 2022

The study aimed at assessing physical fitness and occurrence of the frailty syndrome among social welfare homes’ residents as well as defining factors which determine the level of frailty and its occurrence. The examination included 198 residents (115 females and 83 males of average age 75.5 ± 10.21) and was carried out with the use of the Short Physical Performance Battery (SPPB) test with the following cut-off points: 0–6—frail, 7–9—pre-frail, 10–12—non-frail. The research additionally collected data regarding age, gender, number of chronic diseases, education level, type of prior work and current physical activity. In addition, the height and weight of the respondents were measured. The frailty syndrome was found in more than a half of the examinees (104; 52.53%), the pre-frailty state in 30.30% (n = 60) and 17.17% (n = 34) were non-frail. The average result of the SPPB test was 6.52 ± 2.73, which proves a moderate limitation of the sample group’s fitness. No significant differences were noted between female and male respondents (p = 0.27). The multifactorial linear regression model showed that independent and direct frailty syndrome predicators included age, number of chronic diseases and regular physical activity (p < 0.05). In conclusion, promoting and encouraging regular, age and interest-related forms of physical activity among seniors might foster the maintenance of their physiological reservoir and functional efficiency.
Spatiotemporal Distribution and Evolution of Digestive Tract Cancer Cases in Lujiang County, China since 2012
Authors: Ma Kang , Lin Yuesheng , Zhang Xiaopeng , Fang Fengman , Zhang Yong , Li Jiajia , Yao Youru , Ge Lei , Tan Huarong , Wang Fei
Published: June 2022

This study aims to analyze the spatiotemporal distribution and evolution of digestive tract cancer (DTC) in Lujiang County, China by using the geographic information system technology. Results of this study are expected to provide a scientific basis for effective prevention and control of DTC. The data on DTC cases in Lujiang County, China, were downloaded from the Data Center of the Center for Disease Control and Prevention in Hefei, Anhui Province, China, while the demographic data were sourced from the demographic department in China. Systematic statistical analyses, including the spatial empirical Bayes smoothing, spatial autocorrelation, hotspot statistics, and Kulldorff’s retrospective space-time scan, were used to identify the spatial and spatiotemporal clusters of DTC. GM(1,1) and standard deviation ellipses were then applied to predict the future evolution of the spatial pattern of the DTC cases in Lujiang County. The results showed that DTC in Lujiang County had obvious spatiotemporal clustering. The spatial distribution of DTC cases increases gradually from east to west in the county in a stepwise pattern. The peak of DTC cases occurred in 2012–2013, and the high-case spatial clusters were located mainly in the northwest of Lujiang County. At the 99% confidence interval, two spatiotemporal clusters were identified. From 2012 to 2017, the cases of DTC in Lujiang County gradually shifted to the high-incidence area in the northwest, and the spatial distribution range experienced a process of “dispersion-clustering”. The cases of DTC in Lujiang County will continue to move to the northwest from 2018 to 2025, and the predicted spatial clustering tends to be more obvious.
Application of EMGB to Study Impacts of Public Green Space on Active Transport Behavior: Evidence from South Korea
Authors: Kim Myung Ja , Hall C. Michael
Published: June 2022

Public green spaces (e.g., parks, green trails, greenways) and motivations to engage in active transport are essential for encouraging walking and cycling. However, how these key factors influence walker and cyclist behavior is potentially being increasingly influenced by the use of smart apps, as they become more ubiquitous in everyday practices. To fill this research gap, this work creates and tests a theoretically integrated study framework grounded in an extended model of goal-directed behavior, including public green space and motivation with perceived usefulness of smart apps. In order to accomplish the purpose of this study, we conducted an online survey of Korean walkers (n = 325) and cyclists (n = 326) between 10 and 25 July 2021 and applied partial least squares, structural equation, and multi-group analysis to validate the research model. Results revealed that active transport users’ awareness of public green space positively influences attitude toward (γ = 0.163), as well as behavioral intention of (γ = 0.159), walking and cycling. Additionally, motivation (extrinsic and intrinsic) greatly influences attitude (γ = 0.539) and behavioral intention (γ = 0.535). Subjective norms (γ = 0.137) and positive (γ = 0.466) and negative anticipated emotions (γ = 0.225) have a significant impact on the desire that leads to behavioral intention. High and low perceived smart app usefulness also significantly moderates between public green space and attitude (t-value = 25.705), public green space and behavioral intention (t-value = 25.726), motivation and attitude (t-value = −25.561), and motivation and behavioral intention (t-value = −15.812). Consequently, the findings are useful to academics and practitioners by providing new knowledge and insights.
An Audit and Survey of Informal Use of Instant Messaging for Dermatology in District Hospitals in KwaZulu-Natal, South Africa
Authors: Morris Christopher , Scott Richard E. , Mars Maurice
Published: June 2022

Background. In KwaZulu-Natal (KZ-N), South Africa, recent reports have indicated that spontaneous use of smartphones has occurred, providing access to specialist dermatological care to remote areas. This informal use has raised a number of practical, legal, regulatory, and ethical concerns. Aim. To assess the nature and content of WhatsApp messages sent to dermatologists, to determine the referring doctors’ reasons for, and satisfaction with, their interactions, as well as their knowledge of legal, regulatory, and ethical requirements. Methods. A retrospective study of WhatsApp messages between referring doctors and dermatologists, as well as a cross-sectional survey of doctors working at district hospitals in KZ-N who used IM for teledermatology. Results. Use of IM (primarily WhatsApp) for teledermatology was almost universal, but often not considered ‘telemedicine’. Few referring doctors were aware of South Africa’s ethical guidelines and their requirements, and few of those who did followed them, e.g., the stipulated and onerous consent process and existing privacy and security legislations. No secure methods for record keeping or data storage of WhatsApp content were used. A desire to formalize the service existed. Conclusions. Based upon these findings, it was proposed that a number of described steps be followed in order to formalize the use of IM for teledermatology.
An Explanatory Model of the Relationships between Physical Activity, Social Support and Screen Time among Adolescents
Authors: Sanz-Martín Daniel , Melguizo-Ibáñez Eduardo , Ruiz-Tendero Germán , Ubago-Jiménez José Luis
Published: June 2022

Effective physical activity studies are necessary to understand how factors involved in physical activity mediate behaviour. Therefore, more reliable explanatory models can be generated in order to design effective actions to promote physical activity. The study had two aims: (1) to develop an explanatory model to identify and establish the relationships between physical activity, social support and screen time among adolescents; and (2) to contrast the explanatory structural model by means of a multi-group analysis according to sex. The study design was cross-sectional with descriptive and correlational analysis. The research was carried out with a representative sample of adolescents from the province of Soria (mean age 14.06 ± 1.27 years). The instruments used were: Four by One-Day Physical Activity Questionnaire, Parent Support Scale and Peer Support Scale. The results show that social support had a negative relationship with screen time (r = −0.178; p ≤ 0.001); males had a positive relationship between physical activity and screen time (r = 0.118; p ≤ 0.05); and peer support had more influence on social support than parental support. In conclusion, the proposed model was effective in triangulating the relationships between physical activity, social support and screen time in a novel way, while allowing us to discriminate these results according to participants’ sex.
ICT as a Key Determinant of Efficiency: A Bootstrap-Censored Quantile Regression (BCQR) Analysis for Vietnamese Banks
Authors: Le Tu D. Q. , Ngo Thanh , Ho Tin H. , Nguyen Dat T.
Published: June 2022

There is evidence that ICT developments can improve bank efficiency and performance. Previous studies often employ data envelopment analysis (DEA) to first examine bank performance and then use a second-stage regression to explain the influences of other environmental factors, including ICT, on such efficiency. Since DEA efficiency scores are bounded between the (0, 1] intervals, Tobit and truncated regressions are commonly used in this stage. However, none has accounted for the skewness characteristic of DEA efficiency. This paper applied a bootstrap-censored quantile regression (BCQR) approach to triply account for the issues of a small sample (via bootstrap), bounded intervals (via censored regression), and skewness (via quantile regression) in DEA analysis. We empirically examined the efficiency and performance of 27 Vietnamese commercial banks in the 2007–2019 period. The efficiency scores derived from our first stage revealed that they are skewed and thus, justify the use of the BCQR in the second stage. The BCQR results further confirmed that ICT developments could enhance bank efficiency, which supports the recent policy to restructure the Vietnamese banking sector toward innovation and digitalization. We also examined the impacts of other factors such as bank ownership, credit risk, and bank size on efficiency.
Dynamic Factor Rotation Strategy: A Business Cycle Approach
Authors: Kwon Dohyoung
Published: June 2022

This study developed an investment framework to implement dynamic factor rotation strategies according to changes in economic conditions. I constructed a useful macro indicator that tracked real-time business cycles of the US economy and applied a trend-filtering method to the indicator to identify economic regimes based on the level and momentum change. I found that historical performance of individual equity factors greatly differed across economic regimes, and this heterogeneity can be exploited to build dynamic factor rotation strategies by shifting exposures toward effective factors according to the different regimes. The out-of-sample analysis showed that the regime-based dynamic approach outperformed the static benchmark in terms of absolute and risk-adjusted returns after accounting for transaction costs. The results have important implications for many pension funds and for institutional investors interested in factor investing and seeking to improve the long-term portfolio performance.
Applying Decision Trees to Examine the Nonlinear Effects of Multiscale Transport Accessibility on Rural Poverty in China
Authors: Cui Leibo , Li Tao , Qiu Menglong , Cao Xiaoshu
Published: June 2022

Accessibility plays an important role in alleviating rural poverty. Previous studies have explored the relationship between accessibility and rural poverty, but they offer limited evidence of the collective influence of multiscale transport accessibility (town-level, county-level, and prefecture-level accessibility) and its nonlinear effects on rural poverty. This study adopted the gradient-boosting decision tree model to explore the nonlinear association and threshold effects of multiscale transport accessibility on the rural poverty incidence (RPI). We selected Huining, a poverty-stricken county in China, as a case study. The results show that multiscale transport accessibility collectively has larger predictive power than other variables. Specifically, town-level accessibility (12.97%) plays a dominant role in predicting the RPI, followed by county-level accessibility (9.50%) and prefecture-level accessibility (7.38%). We further identified the nonlinear association and effective ranges of multiscale transport accessibility to guide poverty-alleviation policy. Our results help inform policy and planning on sustainable poverty reduction and rural vitalization.
A Novel Triple-Action Inhibitor Targeting B-Cell Receptor Signaling and BRD4 Demonstrates Preclinical Activity in Chronic Lymphocytic Leukemia
Authors: Smith Audrey L. , Eiken Alexandria P. , Skupa Sydney A. , Moore Dalia Y. , Umeta Lelisse T. , Smith Lynette M. , Lyden Elizabeth R. , D’Angelo Christopher R. , Kallam Avyakta , Vose Julie M. , Kutateladze Tatiana G. , El-Gamal Dalia
Published: June 2022

B-cell chronic lymphocytic leukemia (CLL) results from intrinsic genetic defects and complex microenvironment stimuli that fuel CLL cell growth through an array of survival signaling pathways. Novel small-molecule agents targeting the B-cell receptor pathway and anti-apoptotic proteins alone or in combination have revolutionized the management of CLL, yet combination therapy carries significant toxicity and CLL remains incurable due to residual disease and relapse. Single-molecule inhibitors that can target multiple disease-driving factors are thus an attractive approach to combat both drug resistance and combination-therapy-related toxicities. We demonstrate that SRX3305, a novel small-molecule BTK/PI3K/BRD4 inhibitor that targets three distinctive facets of CLL biology, attenuates CLL cell proliferation and promotes apoptosis in a dose-dependent fashion. SRX3305 also inhibits the activation-induced proliferation of primary CLL cells in vitro and effectively blocks microenvironment-mediated survival signals, including stromal cell contact. Furthermore, SRX3305 blocks CLL cell migration toward CXCL-12 and CXCL-13, which are major chemokines involved in CLL cell homing and retention in microenvironment niches. Importantly, SRX3305 maintains its anti-tumor effects in ibrutinib-resistant CLL cells. Collectively, this study establishes the preclinical efficacy of SRX3305 in CLL, providing significant rationale for its development as a therapeutic agent for CLL and related disorders.
Lysine Deprivation Regulates Npy Expression via GCN2 Signaling Pathway in Mandarin Fish (Siniperca chuatsi)
Authors: Zou Jia-Ming , Zhu Qiang-Sheng , Liang Hui , Lu Hai-Lin , Liang Xu-Fang , He Shan
Published: June 2022

Regulation of food intake is associated with nutrient-sensing systems and the expression of appetite neuropeptides. Nutrient-sensing systems generate the capacity to sense nutrient availability to maintain energy and metabolism homeostasis. Appetite neuropeptides are prominent factors that are essential for regulating the appetite to adapt energy status. However, the link between the expression of appetite neuropeptides and nutrient-sensing systems remains debatable in carnivorous fish. Here, with intracerebroventricular (ICV) administration of six essential amino acids (lysine, methionine, tryptophan, arginine, phenylalanine, or threonine) performed in mandarin fish (Siniperca chuatsi), we found that lysine and methionine are the feeding-stimulating amino acids other than the reported valine, and found a key appetite neuropeptide, neuropeptide Y (NPY), mainly contributes to the regulatory role of the essential amino acids on food intake. With the brain cells of mandarin fish cultured in essential amino acid deleted medium (lysine, methionine, histidine, valine, or leucine), we showed that only lysine deprivation activated the general control nonderepressible 2 (GCN2) signaling pathway, elevated α subunit of eukaryotic translation initiation factor 2 (eIF2α) phosphorylation, increased activating transcription factor 4 (ATF4) protein expression, and finally induced transcription of npy. Furthermore, pharmacological inhibition of GCN2 and eIF2α phosphorylation signaling by GCN2iB or ISRIB, effectively blocked the transcriptional induction of npy in lysine deprivation. Overall, these findings could provide a better understanding of the GCN2 signaling pathway involved in food intake control by amino acids.
Changes in Rhizosphere Soil Fungal Communities of Pinus tabuliformis Plantations at Different Development Stages on the Loess Plateau
Authors: Wang Jiaxing , Gao Jing , Zhang Haoqiang , Tang Ming
Published: June 2022

The soil fungal community is an important factor in the forest ecosystems, and a better understanding of its composition and dynamic changes will contribute to the maintenance, preservation, and sustainable development of the forest ecosystems. Pinus tabuliformis has been widely planted for local ecological restoration on the Loess Plateau in China in recent decades. However, these plantations have been degraded to different degrees with increasing stand age. Hence, we tried to find the possible causes for the plantation degradation by analyzing soil environmental changes and soil fungal community composition at different stand ages. We collected rhizosphere soil samples from young (10-year-old), middle-aged (20-year-old), and near-mature (30-year-old) P. tabuliformis plantations in this region and characterized their soil properties and soil fungal community diversity and composition. Our results showed that with increasing stand age, the contents of organic carbon, ammonium nitrogen (AN) and nitrate nitrogen (NN) in the soil increased significantly, while the content of available phosphorus (AP) decreased significantly. The main factors affecting the composition of the soil fungal community were the contents of AP, AN, and NN in the soil. In addition, the genus Suillus was the dominant ectomycorrhizal (ECM) fungus in all periods of P. tabuliformis plantations in this region. The results of structural equation modeling showed that the community composition of ECM fungi was significantly correlated with stand age, soil NN, and AP contents, and that of pathogenic (PAG) fungi was significantly correlated with soil AN and AP contents. The decrease in the relative abundance of ECM fungi and the increase in the relative abundance of PAG fungi would exacerbate the degradation of P. tabulaeformis plantation. Our results illustrated that the content of soil AP is not only an important factor limiting the development of plantations, but it also significantly affects the community composition of soil fungi in the rhizosphere of the P. tabuliformis plantation. This study provides a novel insight into the degradation of P. tabuliformis plantations and builds a solid foundation for their subsequent management, restoration, and sustainable development on the Loess Plateau of China.
Plant Biology and Biotechnology: Focus on Genomics and Bioinformatics
Authors: Orlov Yuriy L. , Ivanisenko Vladimir A. , Dobrovolskaya Oxana B. , Chen Ming
Published: June 2022

The study of molecular mechanisms of plant stress response is important for agrobiotechnology applications as it was discussed at series of recent bioinformatics conferences [...]
Host Range, Morphology and Sequence Analysis of Ten Temperate Phages Isolated from Pathogenic Yersinia enterocolitica Strains
Authors: Hammerl Jens Andre , El-Mustapha Sabrin , Bölcke Michelle , Trampert Hannah , Barac Andrea , Jäckel Claudia , Gadicherla Ashish K. , Hertwig Stefan
Published: June 2022

Yersinia enterocolitica is a heterogeneous species comprising highly pathogenic, weakly pathogenic and non-pathogenic strains. Previous data suggest that gene exchange may occur in Yersinia. Only scarce information exists about temperate phages of Y. enterocolitica, even though many prophage sequences are present in this species. We have examined 102 pathogenic Y. enterocolitica strains for the presence of inducible prophages by mitomycin C treatment. Ten phages were isolated from nine strains belonging to the bio (B)/serotypes (O) B2/O:5,27, B2/O:9 and 1B/O:8. All phages are myoviruses showing lytic activity only at room temperature. Whole-genome sequencing of the phage genomes revealed that they belong to three groups, which, however, are not closely related to known phages. Group 1 is composed of five phages (type phage: vB_YenM_06.16.1) with genome sizes of 43.8 to 44.9 kb, whereas the four group 2 phages (type phage: vB_YenM_06.16.2) possess smaller genomes of 29.5 to 33.2 kb. Group 3 contains only one phage (vB_YenM_42.18) whose genome has a size of 36.5 kb, which is moderately similar to group 2. The host range of the phages differed significantly. While group 1 phages almost exclusively lysed strains of B2/O:5,27, phages of group 2 and 3 were additionally able to lyse B4/O:3, and some of them even B2/O:9 and 1B/O:8 strains.
The Value of FET PET/CT in Recurrent Glioma with a Different IDH Mutation Status: The Relationship between Imaging and Molecular Biomarkers
Authors: Skoblar Vidmar Marija , Doma Andrej , Smrdel Uroš , Zevnik Katarina , Studen Andrej
Published: June 2022

The evaluation of treatment response remains a challenge in glioma cases because the neuro oncological therapy can lead to the development of treatment-related changes (TRC) that mimic true progression (TP). Positron emission tomography (PET) using O-(2-[18F] fluoroethyl-)-L-tyrosine (18F-FET) has been shown to be a useful tool for detecting TRC and TP. We assessed the diagnostic performance of different 18F-FET PET segmentation approaches and different imaging biomarkers for differentiation between late TRC and TP in glioma patients. Isocitrate dehydrogenase (IDH) status was evaluated as a predictor of disease outcome. In our study, the proportion of TRC in IDH wild type (IDHwt) and IDH mutant (IDHm) subgroups was without significant difference. We found that the diagnostic value of static and dynamic biomarkers of 18F-FET PET for discrimination between TRC and TP depends on the IDH mutation status of the tumor. Dynamic 18F-FET PET acquisition proved helpful in the IDH wild type (IDHwt) subgroup, as opposed to the IDH mutant (IDHm) subgroup, providing an early indication to discontinue dynamic imaging in the IDHm subgroup.
Is the COVID-19 Pandemic Over? The Current Status of Boosters, Immunosenescence, Long Haul COVID, and Systemic Complications
Authors: Ting Miriam , Suzuki Jon B.
Published: June 2022

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), appears to be diminishing in infectivity and hospitalizations in the United States and many parts of the world. This review will provide current information on the pathogenesis of SARS-CoV-2 and long haul COVID, emerging research on systemic complications, and antibody responses of vaccines and boosters.
Evaluation of the Clinical Effects of an Antiviral, Immunostimulant and Antioxidant Phytotherapy in Patients Suffering from COVID-19 Infection: An Observational Pilot Study
Authors: Ortore Massimiliano , Grazioli Elisa , Tranchita Eliana , Minganti Carlo , Manteca Alessia , Tinto Ludovico , Cerulli Claudia , Fabi Igino , Foti Antonella , Borriello Giovanna , Riondino Paolo , Parisi Attilio
Published: June 2022

Background: In the last two years, the COVID-19 pandemic has spread all over the world, affecting millions of people. The same infection can manifest in different clinical conditions, ranging from mild situations to severe patient impairment, up to their death. The COVID-19 infection can activate innate and adaptive immune systems and cause massive inflammatory responses that is important to treat as soon as possible. Methods: In the initial phase of the pandemic, a group of 240 unvaccinated subjects with COVID-19 disease was administered phytotherapy with immunostimulant and antioxidant property to evaluate the role of this phytotherapeutic preparation in counteracting the progression of the COVID-19 disease both in duration and complexity. Results: 161 patients were treated with phytotherapy alone and the prevailing symptoms in the acute phase were rhinitis, fever, cough, osteo-muscular pains; the other 79 patients were given a therapy with NSAIDs, symptomatic drugs, monoclonal antibodies, corticosteroids, antibiotics, and/or heparin. The coexistence of comorbidity (such as diabetes, hypertension, gastro-intestinal disease) was recorded in 74 out of 240 subjects, more frequently in the older subjects; there was no statistically significant correlation between the presence of comorbidity and the duration of disease. Hospitalization rate in this population was 1.6% and lethality rate was 0%. Conclusion: The use of phytotherapy can represent a valid weapon against COVID-19, since it showed no side effects and can also be used in association with other pharmacological therapies to reduce the massive inflammatory responses of this infection.
LoRaWAN Based Indoor Localization Using Random Neural Networks
Authors: Ingabire Winfred , Larijani Hadi , Gibson Ryan M. , Qureshi Ayyaz-UI-Haq
Published: June 2022

Global Positioning Systems (GPS) are frequently used as a potential solution for localization applications. However, GPS does not work indoors due to a lack of direct Line-of-Sight (LOS) satellite signals received from the End Device (ED) due to thick solid materials blocking the ultra-high frequency signals. Furthermore, fingerprint localization using Received Signal Strength Indicator (RSSI) values is typical for localization in indoor environments. Therefore, this paper develops a low-power intelligent localization system for indoor environments using Long-Range Wide-Area Networks (LoRaWAN) RSSI values with Random Neural Networks (RNN). The proposed localization system demonstrates 98.5% improvement in average localization error compared to related studies with a minimum average localization error of 0.12 m in the Line-of-Sight (LOS). The obtained results confirm LoRaWAN-RNN-based localization systems suitable for indoor environments in LOS applied in big sports halls, hospital wards, shopping malls, airports, and many more with the highest accuracy of 99.52%. Furthermore, a minimum average localization error of 13.94 m was obtained in the Non-Line-of-Sight (NLOS) scenario, and this result is appropriate for the management and control of vehicles in indoor car parks, industries, or any other fleet in a pre-defined area in the NLOS with the highest accuracy of 44.24%.
Life-History Traits from Embryonic Development to Reproduction in the American Cockroach
Authors: Lin Liangguan , Wen Jiazhen , Li Sheng , Liu Fangfang
Published: June 2022

The American cockroach, Periplaneta americana (Insecta: Blattodea: Solumblattodea: Blattidae), is an urban hygiene pest but also a model organism for physiology and neuroscience study. However, the current description of the developmental process of the American cockroach is insufficient. In this study, we provide a life table of the American cockroach in a stable environment, including embryonic development, nymphal instars and adult reproduction. Our results show that there are 14 nymphal instars of the American cockroach in groups with sufficient living materials and space. The secondary sexual characteristics are evident in last-instar nymphs and adults, namely, the complete absence of the anal stylus in females. The entire embryonic development process was divided into 20 stages on the basis of lateral-view observations of the embryos. The formation of the embryo involves the fusion of paired blastoderm regions with higher cellular density, similar to that in other insects of Polyneoptera. With respect to reproduction, the gamogenetic females produced their first ootheca earlier than the parthenogenic females, and the frequency of oviposition was higher for the former throughout adulthood. Interestingly, group living seems to improve the parthenogenesis success rate in the American cockroach.
Optimizing the Use of Basil as a Functional Plant for the Biological Control of Aphids by Chrysopa pallens (Neuroptera: Chrysopidae) in Greenhouses
Authors: Fang Yan , Li Shu , Xu Qingxuan , Wang Jie , Yang Yajie , Mi Yingying , Jin Zhenyu , Desneux Nicolas , Wang Su
Published: June 2022

Effective biological control agents that can provide sustainable pest control need to be researched in further detail; functional plants (or non-crop insectary plants), in particular, are garnering increased research interest. Much remains to be learned as to how non-crop plants can augment biological control in greenhouse systems. In this study, we combined laboratory and greenhouse assays to assess the extent to which basil (Ocimum basilicum L.) (Lamiales: Lamiaceae) affected the biological control of aphids by the predatory lacewing Chrysopa pallens (Rambur) (Neuroptera: Chrysopidae). In the presence of the target prey (peach aphid; Myzus persicae (Sulzer)), both the vegetative and flowering stages of basil enhanced C. pallens longevity and (early-age) fecundity as compared to a control treatment. When basil plants were established near aphid infested eggplants (Solanum melongena L.), the C. pallens colonization rate improved by 72–92% in the short-term. Lacewing colonization patterns were modulated by the basil planting density and spatial arrangement (i.e., perimeter planting vs. intercropping). Under high density intercrop arrangements, C. pallens colonization rates were highest, its populations persisted longer in the crop, and the aphid numbers declined more rapidly. Our work shows how basil enhanced the key fitness attributes of a generalist predatory lacewing and benefitted aphid biological control in a greenhouse setting.
Infective Endocarditis in the Elderly: Challenges and Strategies
Authors: Bea Carlos , Vela Sara , García-Blas Sergio , Perez-Rivera Jose-Angel , Díez-Villanueva Pablo , de Gracia Ana Isabel , Fuertes Eladio , Oltra Maria Rosa , Ferrer Ana , Belmonte Andreu , Santas Enrique , Pellicer Mauricio , Colomina Javier , Doménech Alberto , Bodi Vicente , Forner Maria José , Chorro Francisco Javier , Bonanad Clara
Published: June 2022

The specific management of infective endocarditis (IE) in elderly patients is not specifically addressed in recent guidelines despite its increasing incidence and high mortality in this population. The term “elderly” corresponds to different ages in the literature, but it is defined by considerable comorbidity and heterogeneity. Cancer incidence, specifically colorectal cancer, is increased in older patients with IE and impacts its outcome. Diagnosis of IE in elderly patients is challenging due to the atypical presentation of the disease and the lower performance of imaging studies. Enterococcal etiology is more frequent than in younger patients. Antibiotic treatment should prioritize diminishing adverse effects and drug interactions while maintaining the best efficacy, as surgical treatment is less commonly performed in this population due to the high surgical risk. The global assessment of elderly patients with IE, with particular attention to frailty and geriatric profiles, should be performed by multidisciplinary teams to improve disease management in this population.
Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes
Authors: Baran Jakub , Niewiara Łukasz , Podolec Jakub , Siedliński Mateusz , Józefczuk Ewelina , Bernacik Anna , Badacz Rafał , Przewłocki Tadeusz , Pieniążek Piotr , Żmudka Krzysztof , Legutko Jacek , Kabłak-Ziembicka Anna
Published: June 2022

Background: Although degenerative aortic valve stenosis (DAS) is the most prevalent growth-up congestive heart valve disease, still little known about relationships between DAS severity, vascular stiffness (VS), echocardiographic parameters, and serum biomarkers in patients undergoing transcatheter (TAVR) or surgical aortic valve replacement (SAVR). The objective of this study was to identify biomarkers associated with DAS severity, and those that are associated with cardiovascular death (CVD) and episodes of chronic heart failure (CHF) exacerbation. Methods: A total of 137 patients with initially moderate-to-severe DAS were prospectively evaluated for the relationship between DAS severity, baseline VS, and serum biomarkers (uPAR, GDF-15, Gal-3, IL-6Rα, ET-1, PCSK9, RANTES/CCL5, NT-proBNP, and hs-TnT), and were followed-up for 48 months. The prognostic significance of each variable for CVD and CHF risk was measured by hazard ratio of risk (HR), which was calculated by Cox’s proportional hazard model. Results: DAS severity showed correlations with IL-6Rα (r = 0.306, p < 0.001), uPAR (r = 0.184, p = 0.032), and NT-proBNP (r = −0.389, p < 0.001). Levels of ET-1 and Gal-3 were strongly correlated with VS parameters (r = 0.674, p < 0.001; r = 0.724, p < 0.001). Out of 137 patients, 20 were referred to TAVR, 88 to SAVR, and 29 to OMT. In TAVR patients, the highest levels of ET-1, Gal-3, and VS were found as compared to other patients. The highest incidence of CVD was observed in patients who underwent TAVR (35%), compared to SAVR (8%) and OMT (10.3%) (p = 0.004). In a multivariate analysis, ET-1 occurred predictive of CVD risk (HR 25.1, p = 0.047), while Gal-3 > 11.5 ng/mL increased the risk of CHF exacerbation episodes requiring hospital admission by 12%. Conclusions: Our study indicated that ET-1 and Gal-3 levels may be associated with the outcomes in patients with DAS.
Determinants of Prolonged Length of Hospital Stay in Patients with Severe Acute Ischemic Stroke
Authors: Lin Kuan-Hung , Lin Huey-Juan , Yeh Poh-Shiow
Published: June 2022

Objective: Long hospitalizations are associated with a high comorbidity and considerable hospital cost. Admissions of severe acute ischemic stroke are prone to longer hospitalizations. We aimed to explore the issue and method for improving the length of stay. Methods: From the prospective Stroke Registry between January 2019 and June 2020, acute ischemic strokes with an admission National Institutes of Health Stroke Scale ≥ 15 were identified. Prolonged length-of-stay was defined as in-hospital-stay ≥ 30 days. All clinical characteristics were collected, and all do-not-resuscitate documentations were categorized if the order had been written within 7 days of onset. Results: A total of 212 patients were eligible for severe stroke. Of these, 42 (19.8%) had prolonged length-of-stay and 170 had non-prolonged length-of-stay (median 43 vs. 13 days). The prolonged group was younger, mostly men, and was more likely to be in an independent state and more likely to receive reperfusion therapy, and there was a higher frequency of late do-not-resuscitate orders if signed. Although there was a lower in-hospital mortality rate in the prolonged group (12% vs. 23%), there was a higher proportion with a severe functional state (Modified Rankin Scale = 4–5) among the survivors (97% vs. 87%). Conclusions: Severe acute ischemic stroke patients with a prolonged length-of-stay were younger, mostly male, more likely to receive reperfusion therapy, less likely to have an early do-not-resuscitate order if signed, and more likely to have poor functional status at discharge, although there was a lower rate of in-hospital mortality.