bluuebunny/crossref_metadata_2025 huggingface dataset:
Lactobacillus Kefiri LKF01 (Kefibios®) for Prevention of Diarrhoea in Cancer Patients Treated with Chemotherapy: A Prospective Study
Authors: Ghidini Michele , Nicoletti Mariaceleste , Ratti Margherita , Tomasello Gianluca , Lonati Veronica , Ghilardi Mara , Parati Maria Chiara , Borgonovo Karen , Cabiddu Mary , Petrelli Fausto
Published: January 2021

Diarrhoea is one of the main side effects that cancer patients face. The literature showsthat the incidence of chemotherapy (CT)-induced diarrhoea (grade 3–4) in treated patients is in the range of 10–20%, particularly after 5-fluorouracil (5-FU) bolus or some combination therapies of irinotecan and fluoropyrimidines. The aim of the present study was to evaluate the clinical effectiveness of Lactobacillus kefiri LKF01 (Kefibios®) in the prevention or treatment of CT-related diarrhoea in the cancer population. We conducted a prospective observational study. Patients enrolled were adults treated for at least four months with 5-FU-based CT. Kefibios® was administered to patients every day. The primary outcome was the evaluation of the incidence of grade 3–4 CT-induced diarrhoea. We included 76 patients in the final analysis. A 6.6% incidence of high-grade diarrhoea was found in the evaluated population (4.7% of patients treated with 5-FU-based therapy and 8.5% of patients treated with capecitabine-based CT). The overall incidence of high-grade diarrhoea observed was higher in the 1st and 2nd cycles (3.9%), with a subsequent sharp reduction from the 3rd cycle (1.3%) and negativisation from the 5th cycle. Lactobacillus kefiri LKF01 (Kefibios®) is safe and effective in preventing severe diarrhoea in cancer patients receiving 5-FU or capecitabine-based treatment.
Berry-Derived Polyphenols in Cardiovascular Pathologies: Mechanisms of Disease and the Role of Diet and Sex
Authors: Najjar Rami S. , Turner Casey G. , Wong Brett J. , Feresin Rafaela G.
Published: January 2021

Cardiovascular disease (CVD) prevalence, pathogenesis, and manifestation is differentially influenced by biological sex. Berry polyphenols target several signaling pathways pertinent to CVD development, including inflammation, oxidative stress, and cardiac and vascular remodeling, and there are innate differences in these pathways that also vary by sex. There is limited research systematically investigating sex differences in berry polyphenol effects on these pathways, but there are fundamental findings at this time that suggest a sex-specific effect. This review will detail mechanisms within these pathological pathways, how they differ by sex, and how they may be individually targeted by berry polyphenols in a sex-specific manner. Because of the substantial polyphenolic profile of berries, berry consumption represents a promising interventional tool in the treatment and prevention of CVD in both sexes, but the mechanisms in which they function within each sex may vary.
Neural Coding of Food Is a Multisensory, Sensorimotor Function
Authors: Di Lorenzo Patricia M.
Published: January 2021

This review is a curated discussion of the relationship between the gustatory system and the perception of food beginning at the earliest stage of neural processing. A brief description of the idea of taste qualities and mammalian anatomy of the taste system is presented first, followed by an overview of theories of taste coding. The case is made that food is encoded by the several senses that it stimulates beginning in the brainstem and extending throughout the entire gustatory neuraxis. In addition, the feedback from food-related movements is seamlessly melded with sensory input to create the representation of food objects in the brain.
Sex May Modulate the Effects of Combined Polyphenol Extract and L-citrulline Supplementation on Ambulatory Blood Pressure in Adults with Prehypertension: A Randomized Controlled Trial
Authors: Vors Cécile , Rancourt-Bouchard Maryka , Couillard Charles , Gigleux Iris , Couture Patrick , Lamarche Benoît
Published: January 2021

Increased blood pressure (BP), vascular dysfunction and inflammation are involved in the etiology of cardiovascular disease (CVD). Although several dietary components such as polyphenols and L-citrulline may help to control BP, their combined impact on ambulatory BP in individuals at risk of CVD remains unknown. The objective of this research was to investigate the short-term impact of supplementation with a combination of polyphenol extract and L-citrulline on ambulatory BP, endothelial function and inflammation. In a randomized double-blind parallel trial, 73 men and women with prehypertension were supplemented with a placebo (cellulose, n = 34, Plac) or 548 mg/day of polyphenols and 2 g/day of L-citrulline (n = 35, Suppl) for 6 weeks. The primary outcome of this study was the difference between groups in 24-h ambulatory diastolic BP (DBP) at week six. Secondary outcomes were a difference between groups at week six in ambulatory systolic BP (SBP), casual BP, serum lipids and high-sensitivity C-reactive protein (hs-CRP) concentrations and skin advanced glycation end products (AGEs). Potential interaction of treatment with sex was examined. Suppl had no impact on mean ambulatory SBP and DBP (p > 0.10 vs. placebo). Daytime and 24-h SBP were reduced with Suppl in women (p ≤ 0.01), but not in men (p ≥ 0.27). A non-significant reduction in AGEs was observed after Suppl compared to Plac among all participants (p = 0.07) and there was no difference in the concentrations of blood lipids (p > 0.20) or CRP (p = 0.36) between treatments at week six. Therefore, supplementation with polyphenol extract and L-citrulline for 6 weeks has no impact on ambulatory BP, blood lipids and CRP in adults with prehypertension. However, the polyphenol extract/L-citrulline supplement may reduce ambulatory SBP in women, but not in men. These preliminary results need further research efforts towards further documenting this sex-dependent BP response to supplementation with polyphenols and L-citrulline.
Evaluating Alternate Methods of Determining the Antimicrobial Efficacy of Contact Lens Care Products against Acanthamoeba Trophozoites
Authors: Campolo Allison , Shannon Paul , Crary Monica
Published: January 2021

Acanthamoeba keratitis (AK) is a serious ocular infection caused by a ubiquitous free-living amoeba, Acanthamoeba. This infection often results in extensive corneal damage and blindness, and is notoriously difficult to cure. While Acanthamoeba is an abundant organism, AK is most associated with contact lens hygiene noncompliance and inadequate contact lens care (CLC) disinfection regimens. Thus, accurate and timely antimicrobial efficacy testing of CLC solutions is paramount. Published methods for antimicrobial efficacy testing of Acanthamoeba trophozoites requires 14 days for results. Presently, alternate and/or rapid methods for evaluating CLC products rarely demonstrate equivalent results compared to commonly-reported methods. Propidium iodide is a cellular stain that can only bind to cells with damaged outer membranes. We evaluated propidium iodide staining as an alternative method for determining the relative antimicrobial efficacy of 11 different CLC products against Acanthamoeba trophozoites. Following exposure to a CLC product, the fluorescence intensity of propidium iodide in an Acanthamoeba population demonstrated a strong correlation to the log reduction determined by established, growth-based Acanthamoeba testing used to evaluate the antimicrobial efficacy of CLC products. Thus, propidium iodide was found to be an effective rapid tool for determining cell death in Acanthamoeba trophozoites following exposure to CLC solutions.
Prevalence and Phylogenetic Analysis of Microsporidium Enterocytozoon bieneusi in Diarrheal Patients
Authors: Zang Manman , Li Jinjin , Tang Chun , Ding Songtao , Huang Wei , Qin Qizhong , Liu Handeng
Published: January 2021

Enterocytozoon bieneusi can cause severe diarrhea in children and adults. However, in China, there are scant studies on E. bieneusi in diarrheal children and adults, with the exception of prevalence and genotyping data in a small number of cities including Hubei, Shanghai, and Heilongjiang. In this study, 196 fecal samples (n = 132 in Chongqing, n = 44 in Shandong, n = 20 in Hubei) were collected, including 91 from children and 105 from adults. Through microscopic examination, 19 positive samples (11 from children and 8 from adults) were detected. Using PCR examination, the internal transcriptional spacer (ITS) region was utilized by nested PCR to detect and characterize E. bieneusi. Twenty positive samples were detected, including 14 from children (≤11 years of age) and 6 from adults. According to the sequence analysis of ITS data, one known zoonotic (D) and seven novel (CQH5-11) genotypes were identified. This is the first molecular epidemiological study of E. bieneusi in diarrheal patients in different regions of China. Therefore, this study can provide useful information for the molecular epidemiology and control of E. bieneusi infection in humans in the future.
Nitroreductase Activites in Giardia lamblia: ORF 17150 Encodes a Quinone Reductase with Nitroreductase Activity
Authors: Müller Joachim , Heller Manfred , Uldry Anne-Christine , Braga Sophie , Müller Norbert
Published: January 2021

The intestinal diplomonadid Giardia lamblia is a causative agent of persistent diarrhea. Current treatments are based on nitro drugs, especially metronidazole. Nitro compounds are activated by reduction, yielding toxic intermediates. The enzymatic systems responsible for this activation are not completely understood. By fractionating cell free crude extracts by size exclusion chromatography followed by mass spectrometry, enzymes with nitroreductase (NR) activities are identified. The protein encoded by ORF 17150 found in two pools with NR activities is overexpressed and characterized. In pools of fractions with main NR activities, previously-known NRs are identified, as well as a previously uncharacterized protein encoded by ORF 17150. Recombinant protein 17150 is a flavoprotein with NADPH-dependent quinone reductase and NR activities. Besides a set of previously identified NRs, we have identified a novel enzyme with NR activity.
Extracts of the Tiger Milk Mushroom (Lignosus rhinocerus) Enhance Stress Resistance and Extend Lifespan in Caenorhabditis elegans via the DAF-16/FoxO Signaling Pathway
Authors: Kittimongkolsuk Parinee , Roxo Mariana , Li Hanmei , Chuchawankul Siriporn , Wink Michael , Tencomnao Tewin
Published: January 2021

The tiger milk mushroom, Lignosus rhinocerus (LR), exhibits antioxidant properties, as shown in a few in vitro experiments. The aim of this research was to study whether three LR extracts exhibit antioxidant activities in Caenorhabditis elegans. In wild-type N2 nematodes, we determined the survival rate under oxidative stress caused by increased intracellular ROS concentrations. Transgenic strains, including TJ356, TJ375, CF1553, CL2166, and LD1, were used to detect the expression of DAF-16, HSP-16.2, SOD-3, GST-4, and SKN-1, respectively. Lifespan, lipofuscin, and pharyngeal pumping rates were assessed. Three LR extracts (ethanol, and cold and hot water) protected the worms from oxidative stress and decreased intracellular ROS. The extracts exhibited antioxidant properties through the DAF-16/FOXO pathway, leading to SOD-3 and HSP-16.2 modification. However, the expression of SKN-1 and GST-4 was not changed. All the extracts extended the lifespan. They also reduced lipofuscin (a marker for aging) and influenced the pharyngeal pumping rate (another marker for aging). The extracts did not cause dietary restriction. This novel study provides evidence of the functional antioxidant and anti-aging properties of LR. Further studies must confirm that they are suitable for use as antioxidant supplements.
Non-Invasive Low Pulsed Electrical Fields for Inducing BBB Disruption in Mice—Feasibility Demonstration
Authors: Sharabi Shirley , Last David , Daniels Dianne , Fabian Ido Didi , Atrakchi Dana , Bresler Yael , Liraz-Zaltsman Sigal , Cooper Itzik , Mardor Yael
Published: January 2021

The blood–brain barrier (BBB) is a major hurdle for the treatment of central nervous system disorders, limiting passage of both small and large therapeutic agents from the blood stream into the brain. Thus, means for inducing BBB disruption (BBBd) are urgently needed. Here, we studied the application of low pulsed electrical fields (PEFs) for inducing BBBd in mice. Mice were treated by low PEFs using electrodes pressed against both sides of the skull (100–400 square 50 µs pulses at 4 Hz with different voltages). BBBd as a function of treatment parameters was evaluated using MRI-based treatment response assessment maps (TRAMs) and Evans blue extravasation. A 3D numerical model of the mouse brain and electrodes was constructed using finite element software, simulating the electric fields distribution in the brain and ensuring no significant temperature elevation. BBBd was demonstrated immediately after treatment and significant linear regressions were found between treatment parameters and the extent of BBBd. The maximal induced electric field in the mice brains, calculated by the numerical model, ranged between 62.4 and 187.2 V/cm for the minimal and maximal applied voltages. These results demonstrate the feasibility of inducing significant BBBd using non-invasive low PEFs, well below the threshold for electroporation.
Hyaluronidase Inhibitor-Incorporated Cross-Linked Hyaluronic Acid Hydrogels for Subcutaneous Injection
Authors: Kim Min-Hwan , Park Ju-Hwan , Nguyen Duy-Thuc , Kim Sungyun , Jeong Da In , Cho Hyun-Jong , Kim Dae-Duk
Published: January 2021

Hyaluronidase (HAase) inhibitor-incorporated hyaluronic acid (HA) hydrogel cross-linked with 1,4-butanediol diglycidyl ether (BDDE) was designed to reduce the toxicity risk induced by BDDE and its biodegradation rate in subcutaneous tissue. The formulation composition of hydrogel and its preparation method were optimized to have a high swelling ratio and drug content. Quercetin (QCT) and quetiapine (QTP), as an HAase inhibitor and model drug, respectively, were incorporated into the cross-linked hydrogel using the antisolvent precipitation method for extending their release after subcutaneous injection. The cross-linked HA (cHA)-based hydrogels displayed appropriate viscoelasticity and injectability for subcutaneous injection. The incorporation of QCT (as an HAase inhibitor) in the cHA hydrogel formulation resulted in slower in vitro and in vivo degradation profiles compared to the hydrogel without QCT. Single dosing of optimized hydrogel injected via a subcutaneous route in rats did not induce any acute toxicities in the blood chemistry and histological staining studies. In the pharmacokinetic study of rats following subcutaneous injection, the cHA hydrogel with QCT exhibited a lower maximum QTP concentration and longer half-life and mean residence time values compared to the hydrogel without QCT. All of these results support the designed HAase inhibitor-incorporated cHA hydrogel being a biocompatible subcutaneous injection formulation for sustained drug delivery.
47.5 GHz Membrane-III-V-on-Si Directly Modulated Laser for Sub-pJ/bit 100-Gbps Transmission
Authors: Diamantopoulos Nikolaos-Panteleimon (Pandelis) , Yamaoka Suguru , Fujii Takuro , Nishi Hidetaka , Takeda Koji , Tsuchizawa Tai , Kakitsuka Takaaki , Matsuo Shinji
Published: January 2021

Near-future upgrades of intra data center networks and high-performance computing systems would require optical interconnects capable of operating at beyond 100 Gbps/lane. In order for this evolution to be achieved in a sustainable way, high-speed yet energy-efficient transceivers are in need. Towards this direction we have previously demonstrated directly-modulated lasers (DMLs) capable of operating at 50 Gbps/lane with sub-pJ/bit efficiencies based on our novel membrane-III-V-on-Si technology. However, there exists an inherent tradeoff between modulation speed and power consumption due to the carrier-photon dynamics in DMLs. In this work, we alleviate this tradeoff by introducing photon–photon resonance dynamics in our energy-efficient membrane DMLs-on-Si design and demonstrate a device with a maximum 3-dB bandwidth of 47.5 GHz. This denotes a bandwidth increase of more than 2x times compared to our previous membrane DMLs-on-Si. Moreover, the DML is capable of delivering 60-GBaud PAM-4 signals under Ethernet’s KP4-FEC threshold (net data rate of 113.42 Gbps) over 2-km of standard single-mode fiber transmission. DC energy-efficiencies of 0.17 pJ/bit at 25 °C and 0.34 pJ/bit at 50 °C have been achieved for the > 100-Gbps signals. Deploying such DMLs in an integrated multichannel transceiver should ensure a smooth evolution towards Terabit-class Ethernet links and on-board optics subsystems.
Preliminary Identification of Key Genes Controlling Peach Pollen Fertility Using Genome-Wide Association Study
Authors: Huang Zhenyu , Shen Fei , Chen Yuling , Cao Ke , Wang Lirong
Published: January 2021

Previous genetic mapping helped detect a ~7.52 Mb putative genomic region for the pollen fertility trait on peach Chromosome 06 (Chr.06), which was too long for candidate gene characterization. In this study, using the whole-genome re-sequencing data of 201 peach accessions, we performed a genome-wide association study to identify key genes related to peach pollen fertility trait. The significant association peak was detected at Chr.06: 2,116,368 bp, which was in accordance with the previous genetic mapping results, but displayed largely improved precision, allowing for the identification of nine candidate genes. Among these candidates, gene PpABCG26, encoding an ATP-binding cassette G (ABCG) transporter and harboring the most significantly associated SNP (Single Nucleotide Polymorphism) marker in its coding region, was hypothesized to control peach pollen fertility/sterility based on the results of gene function comparison, gene relative expression, and nucleotide sequence analysis. The obtained results will help us to understand the genetic basis of peach pollen fertility trait, and to discover applicable markers for pre-selection in peach.
Morphological, Physiological and Molecular Markers for Salt-Stressed Plants
Authors: Soltabayeva Aigerim , Ongaltay Assel , Omondi John Okoth , Srivastava Sudhakar
Published: January 2021

Plant growth and development is adversely affected by different kind of stresses. One of the major abiotic stresses, salinity, causes complex changes in plants by influencing the interactions of genes. The modulated genetic regulation perturbs metabolic balance, which may alter plant’s physiology and eventually causing yield losses. To improve agricultural output, researchers have concentrated on identification, characterization and selection of salt tolerant varieties and genotypes, although, most of these varieties are less adopted for commercial production. Nowadays, phenotyping plants through Machine learning (deep learning) approaches that analyze the images of plant leaves to predict biotic and abiotic damage on plant leaves have increased. Here, we review salinity stress related markers on molecular, physiological and morphological levels for crops such as maize, rice, ryegrass, tomato, salicornia, wheat and model plant, Arabidopsis. The combined analysis of data from stress markers on different levels together with image data are important for understanding the impact of salt stress on plants.
Corn Starch/Chitosan Nanoparticles/Thymol Bio-Nanocomposite Films for Potential Food Packaging Applications
Authors: Othman Siti Hajar , Othman Nur Fitrah Liyana , Shapi’i Ruzanna Ahmad , Ariffin Siti Hajar , Yunos Khairul Faezah Md.
Published: January 2021

This work aims to develop corn starch/chitosan nanoparticles/thymol (CS/CNP/Thy) bio-nanocomposite films as potential food packaging materials that can enhance the shelf life of food. CS/CNP/Thy bio-nanocomposite films were prepared by the addition of different concentrations of thymol (0, 1.5, 3.0, 4.5 w/w%) using a solvent casting method. The resulting films were characterized in terms of optical, mechanical, and water vapor permeability (WVP) properties. The addition of thymol was found to reduce the tensile strength (TS), elongation at break (EAB), and Young’s modulus (YM) of the films. Generally, the increment in the concentration of thymol did not significantly affect the TS, EAB, and YM values. The addition of 1.5 w/w% thymol increased the WVP of the films but the WVP reduced with the increase in thymol concentrations. CS/CNP/Thy-3% bio-nanocomposite films demonstrated the potential to lengthen the shelf life of cherry tomatoes packed with the films, whereby the cherry tomatoes exhibited no significant changes in firmness and the lowest weight loss. In addition, no mold growth was observed on the sliced cherry tomatoes that were in direct contact with the films during 7 days of storage, proving the promising application of the films as active food packaging materials.
Dipping-Press Coating Method for Retaining Transparency and Imparting Hydrophobicity Regardless of Plastic Substrate Type
Authors: Park Jeeyin , Lim Taekyung , Yang Keun-Hyeok , Ju Sanghyun , Jeong Sang-Mi
Published: January 2021

Plastics are used in cover substrates for billboards, windows, large LED signboards, lighting devices, and solar panels because they are transparent and can be colored and shaped as desired. However, when plastic cover substrates installed in outdoor environments are constantly exposed to harsh conditions such as snow, rain, dust, and wind, their transparency deteriorates owing to watermarks and dust contamination. Herein, we investigated a simple dipping-press coating method that can impart hydrophobicity while maintaining the transparency, regardless of the plastic substrate type. A highly transparent and hydrophobic coating film was formed on a plastic substrate by a two-step process, as follows: (1) application of a polydimethylsiloxane–octadecylamine coating by a dipping process, and (2) embedding (1H,1H,2H,2H-heptadecafluorodec-1-yl) phosphonic acid–aluminum oxide nanoparticles by a thermal press process. The plastic substrates on which the highly transparent and hydrophobic coating film was formed showed 150° or higher hydrophobicity and 80% or higher visible light transparency. The coating method proposed herein can easily impart hydrophobicity and is compatible with any plastic substrate that must maintain prolonged transparency without contamination when exposed to adverse conditions.
Melt- vs. Non-Melt Blending of Complexly Processable Ultra-High Molecular Weight Polyethylene/Cellulose Nanofiber Bionanocomposite
Authors: Sharip Nur Sharmila , Ariffin Hidayah , Yasim-Anuar Tengku Arisyah Tengku , Andou Yoshito , Shirosaki Yuki , Jawaid Mohammad , Tahir Paridah Md , Ibrahim Nor Azowa
Published: January 2021

The major hurdle in melt-processing of ultra-high molecular weight polyethylene (UHMWPE) nanocomposite lies on the high melt viscosity of the UHMWPE, which may contribute to poor dispersion and distribution of the nanofiller. In this study, UHMWPE/cellulose nanofiber (UHMWPE/CNF) bionanocomposites were prepared by two different blending methods: (i) melt blending at 150 °C in a triple screw kneading extruder, and (ii) non-melt blending by ethanol mixing at room temperature. Results showed that melt-processing of UHMWPE without CNF (MB-UHMWPE/0) exhibited an increment in yield strength and Young’s modulus by 15% and 25%, respectively, compared to the Neat-UHMWPE. Tensile strength was however reduced by almost half. Ethanol mixed sample without CNF (EM-UHMWPE/0) on the other hand showed slight decrement in all mechanical properties tested. At 0.5% CNF inclusion, the mechanical properties of melt-blended bionanocomposites (MB-UHMWPE/0.5) were improved as compared to Neat-UHMWPE. It was also found that the yield strength, elongation at break, Young’s modulus, toughness and crystallinity of MB-UHMWPE/0.5 were higher by 28%, 61%, 47%, 45% and 11%, respectively, as compared to the ethanol mixing sample (EM-UHMWPE/0.5). Despite the reduction in tensile strength of MB-UHMWPE/0.5, the value i.e., 28.4 ± 1.0 MPa surpassed the minimum requirement of standard specification for fabricated UHMWPE in surgical implant application. Overall, melt-blending processing is more suitable for the preparation of UHMWPE/CNF bionanocomposites as exhibited by their characteristics presented herein. A better mechanical interlocking between UHMWPE and CNF at high temperature mixing with kneading was evident through FE-SEM observation, explains the higher mechanical properties of MB-UHMWPE/0.5 as compared to EM-UHMWPE/0.5.
Electrospinning Fabrication and Cytocompatibility Investigation of Nanodiamond Particles-Gelatin Fibrous Tubular Scaffolds for Nerve Regeneration
Authors: Olăreț Elena , Drăgușin Diana-Maria , Serafim Andrada , Lungu Adriana , Șelaru Aida , Dobranici Alexandra , Dinescu Sorina , Costache Marieta , Boerașu Iulian , Vasile Bogdan Ștefan , Steinmüller-Nethl Doris , Iovu Horia , Stancu Izabela-Cristina
Published: January 2021

This paper reports the electrospinning fabrication of flexible nanostructured tubular scaffolds, based on fish gelatin (FG) and nanodiamond nanoparticles (NDs), and their cytocompatibility with murine neural stem cells. The effects of both nanofiller and protein concentration on the scaffold morphology, aqueous affinity, size modification at rehydration, and degradation are assessed. Our findings indicate that nanostructuring with low amounts of NDs may modify the fiber properties, including a certain regional parallel orientation of fiber segments. NE-4C cells form dense clusters that strongly adhere to the surface of FG50-based scaffolds, while also increasing FG concentration and adding NDs favor cellular infiltration into the flexible fibrous FG70_NDs nanocomposite. This research illustrates the potential of nanostructured NDs-FG fibers as scaffolds for nerve repair and regeneration. We also emphasize the importance of further understanding the effect of the nanofiller-protein interphase on the microstructure and properties of electrospun fibers and on cell-interactivity.
Design, Modelling, and Experimental Validation of a Scalable Continuous-Flow Hydrothermal Liquefaction Pilot Plant
Authors: Johannsen Ib , Kilsgaard Björn , Milkevych Viktor , Moore Dale
Published: January 2021

In this study, the design and practical implementation of a novel, scalable plug-flow pilot plant for hydrothermal liquefaction of organic feedstock is presented. The overall discussion comprises the system’s design, process modelling, and simulation, as well as results for an experimental validation of the proposed design with a focus on fluid dynamics and heat transfer. The design criteria take into account the scalability of the plug-flow processing system, optimized non-isothermal flow conditions of highly viscous liquids in a tubular system at harsh process conditions, specifically high pressure and medium temperatures, and overall maintenance suitability. A novel forced flow oscillation system as well as unique heat exchange design to reduce the energy consumption during system operation, maximize local flow mixing, and minimize plugging are proposed and experimentally tested. To achieve a better understanding and optimization of Hydrothermal Liquefaction (HTL) (and other) processing systems, a mathematical model of heat transfer coupled with non-isothermal fluid flow was also developed and implemented.
A Review on Temperature Control of Proton Exchange Membrane Fuel Cells
Authors: Li Qinghe , Liu Zhiqiang , Sun Yi , Yang Sheng , Deng Chengwei
Published: January 2021

This paper provides a comprehensive review of the temperature control in proton exchange membrane fuel cells. Proton exchange membrane (PEM) fuel cells inevitably emit a certain amount of heat while generating electricity, and the fuel cell can only exert its best performance in the appropriate temperature range. At the same time, the heat generated cannot spontaneously keep its temperature uniform and stable, and temperature control is required. This part of thermal energy can be classified into two groups. On the one hand, the reaction heat is affected by the reaction process; on the other hand, due to the impedance of the battery itself to the current, the ohmic polarization loss is caused to the battery. The thermal effect of current generates Joule heat, which is manifested by an increase in temperature and a decrease in battery performance. Therefore, it is necessary to design and optimize the battery material structure to improve battery performance and adopt a suitable cooling system for heat dissipation. To make the PEM fuel cell (PEMFC) universal, some extreme situations need to be considered, and a cold start of the battery is included in the analysis. In this paper, the previous studies related to three important aspects of temperature control in proton exchange membrane fuel cells have been reviewed and analyzed to better guide thermal management of the proton exchange membrane fuel cell (PEMFC).
Exploiting the Fruitfly, Drosophila melanogaster, to Identify the Molecular Basis of Cryptochrome-Dependent Magnetosensitivity
Authors: Bradlaugh Adam , Munro Anna L. , Jones Alex R. , Baines Richard A.
Published: January 2021

The flavoprotein CRYPTOCHROME (CRY) is now generally believed to be a magnetosensor, providing geomagnetic information via a quantum effect on a light-initiated radical pair reaction. Whilst there is considerable physical and behavioural data to support this view, the precise molecular basis of animal magnetosensitivity remains frustratingly unknown. A key reason for this is the difficulty in combining molecular and behavioural biological experiments with the sciences of magnetics and spin chemistry. In this review, we highlight work that has utilised the fruit fly, Drosophila melanogaster, which provides a highly tractable genetic model system that offers many advantages for the study of magnetosensitivity. Using this “living test-tube”, significant progress has been made in elucidating the molecular basis of CRY-dependent magnetosensitivity.
Early Grain Cultivation and Starting Processes in the Japanese Archipelago
Authors: Fujio Shin’ichiro
Published: January 2021

This paper presents a specific examination of the introduction of grain cultivation and the processes of development in the Japanese Archipelago. In fact, no definitive archaeological evidence has been found that Jomon hunter–gatherers cultivated grain in the Japanese Archipelago; the earliest potential evidence of grain is a stamp mark of rice on the surface of a final late-Jomon, in about 11th century BC, pottery found at the Itaya 3 site in Shimane Prefecture. Current evidence indicates that the first grain cultivation was started by Jomon people who adopted irrigated wet rice cultivation that had arrived from the Korean Peninsula to northern parts of Kyushu, and gradually spread eastward thereafter. This study specifically examines four regions, including northern Kyushu, Kinki, southern Kanto, and northern Tohoku, in order to investigate the processes of grain cultivation initiation and spread. First, the years during which wet rice cultivation started in each region are estimated based on carbon-14 dating of earthenware types used during that period. Secondly, the timing of the spread of wet rice cultivation has been estimated based on carbon-14 dating of earthenware. Subsequently, differences in the periods between the initiation and dissemination of wet rice cultivation were estimated. Results suggest that dissemination took place over approximately 250 years in northern Kyushu, where wet rice cultivation first started. The time required for adoption decreased gradually as the trend moved eastward. It was estimated to have taken approximately 150 years in Kinki and 20–30 years in southern Kanto, taking place at about the same time. A factor, significantly contributing to such differences in timing and development processes among regions, was likely the relationship between the first farmers who introduced wet rice farming and the indigenous hunter–gatherers who lived there.
The Spatio-Temporal Variability of Frost Blisters in a Perennial Frozen Lake along the Antarctic Coast as Indicator of the Groundwater Supply
Authors: Ponti Stefano , Scipinotti Riccardo , Pierattini Samuele , Guglielmin Mauro
Published: January 2021

Remote sensing, and unmanned aerial vehicles (UAVs) in particular, can be a valid tool for assessing the dynamics of cryotic features as frost blisters and to monitor the surface changes and the sublimation rates on perennially frozen lakes that host important ecosystems. In this paper, through the use of these remote sensing techniques, we aim to understand the type of groundwater supply of an Antarctic perennial frozen lake that encompasses two frost blisters (M1 and M2) through the temporal analysis of the features’ elevation changes (frost blisters and lake ice level). The frozen lake is located at Boulder Clay (northern Victoria Land, Antarctica). We relied on several photogrammetric models, past satellite images and ground pictures to conduct differencing of digital elevation models, areal variations and pixel counting. In addition, in situ measurements of the ice sublimation or snow accumulation were carried out. The two frost blisters showed different elevation trends with M1 higher in the past (1996–2004) than recently (2014–2019), while M2 showed an opposite trend, similarly to the ice level. Indeed, the linear regression between M2 elevation changes and the ice level variation was statistically significant, as well as with the annual thawing degree days, while M1 did not show significant results. From these results we can infer that the groundwater supply of M1 can be related to a sublake open talik (hydraulic system) as confirmed also by pressurized brines found below M1, during a drilling in summer 2019. For M2 the groundwater flow is still not completely clear although the hydrostatic system seems the easiest explanation as well as for the uplift of the lake ice.
Stand Delineation of Pinus sylvestris L. Plantations Suffering Decline Processes Based on Biophysical Tree Crown Variables: A Necessary Tool for Adaptive Silviculture
Authors: Varo-Martínez Mª Ángeles , Navarro-Cerrillo Rafael M.
Published: January 2021

Many planted Pinus forests are severely affected by defoliation and mortality processes caused by pests and droughts. The mapping of forest tree crown variables (e.g., leaf area index and pigments) is particularly useful in stand delineation for the management of declining forests. This work explores the potential of integrating multispectral WorldView-2 (WV-2) and Airborne Laser Scanning (ALS) data for stand delineation based on selected tree crown variables in Pinus sylvestris plantations in southern Spain. Needle pigments (chlorophyll and carotenes) and leaf area index (LAI) were quantified. Eight vegetation indices and ALS-derived metrics were produced, and seven predictors were selected to estimate and map tree crown variables using a Random Forest method and Gini index. Chlorophylls a and b (Chla and Chlb) were significantly higher in the non-defoliated and moderately defoliated trees than in severely defoliated trees (F = 14.02, p < 0.001 for Chla; F = 13.09, p < 0.001 for Chlb). A similar response was observed for carotenoids (Car) (F = 14.13, p < 0.001). The LAI also showed significant differences among the defoliation levels (F = 26.5, p < 0.001). The model for the chlorophyll a pigment used two vegetation indices, Plant Senescence Reflectance Index (PSRI) and Carotenoid Reflectance Index (CRI); three WV-2 band metrics, and three ALS metrics. The model built to describe the tree Chlb content used similar variables. The defoliation classification model was established with a single vegetation index, Green Normalized Difference Vegetation Index (GNDVI); two metrics of the blue band, and two ALS metrics. The pigment contents models provided R2 values of 0.87 (Chla, RMSE = 12.98%), 0.74 (Chlb, RMSE = 10.39%), and 0.88 (Car, RMSE = 10.05%). The cross-validated confusion matrix achieved a high overall classification accuracy (84.05%) and Kappa index (0.76). Defoliation and Chla showed the validation values for segmentations and, therefore, in the generation of the stand delineation. A total of 104 stands were delineated, ranging from 6.96 to 54.62 ha (average stand area = 16.26 ha). The distribution map of the predicted severity values in the P. sylvestris plantations showed a mosaic of severity patterns at the stand and individual tree scales. Overall, the findings of this work underscore the potential of WV-2 and ALS data integration for the assessment of stand delineation based on tree health status. The derived cartography is a relevant tool for developing adaptive silvicultural practices to reduce Pinus sylvestris mortality in planted forests at risk due to climate change.
Consistent Long-Term Monthly Coastal Wetland Vegetation Monitoring Using a Virtual Satellite Constellation
Authors: Tahsin Subrina , Medeiros Stephen C. , Singh Arvind
Published: January 2021

Long-term monthly coastal wetland vegetation monitoring is the key to quantifying the effects of natural and anthropogenic events, such as severe storms, as well as assessing restoration efforts. Remote sensing data products such as Normalized Difference Vegetation Index (NDVI), alongside emerging data analysis techniques, have enabled broader investigations into their dynamics at monthly to decadal time scales. However, NDVI data suffer from cloud contamination making periods within the time series sparse and often unusable during meteorologically active seasons. This paper proposes a virtual constellation for NDVI consisting of the red and near-infrared bands of Landsat 8 Operational Land Imager, Sentinel-2A Multi-Spectral Instrument, and Advanced Spaceborne Thermal Emission and Reflection Radiometer. The virtual constellation uses time-space-spectrum relationships from 2014 to 2018 and a random forest to produce synthetic NDVI imagery rectified to Landsat 8 format. Over the sample coverage area near Apalachicola, Florida, USA, the synthetic NDVI showed good visual coherence with observed Landsat 8 NDVI. Comparisons between the synthetic and observed NDVI showed Root Mean Squared Error and Coefficient of Determination (R2) values of 0.0020 sr−1 and 0.88, respectively. The results suggest that the virtual constellation was able to mitigate NDVI data loss due to clouds and may have the potential to do the same for other data. The ability to participate in a virtual constellation for a useful end product such as NDVI adds value to existing satellite missions and provides economic justification for future projects.
A Novel Squeeze-and-Excitation W-Net for 2D and 3D Building Change Detection with Multi-Source and Multi-Feature Remote Sensing Data
Authors: Zhang Haiming , Wang Mingchang , Wang Fengyan , Yang Guodong , Zhang Ying , Jia Junqian , Wang Siqi
Published: January 2021

Building Change Detection (BCD) is one of the core issues in earth observation and has received extensive attention in recent years. With the rapid development of earth observation technology, the data source of remote sensing change detection is continuously enriched, which provides the possibility to describe the spatial details of the ground objects more finely and to characterize the ground objects with multiple perspectives and levels. However, due to the different physical mechanisms of multi-source remote sensing data, BCD based on heterogeneous data is a challenge. Previous studies mostly focused on the BCD of homogeneous remote sensing data, while the use of multi-source remote sensing data and considering multiple features to conduct 2D and 3D BCD research is sporadic. In this article, we propose a novel and general squeeze-and-excitation W-Net, which is developed from U-Net and SE-Net. Its unique advantage is that it can not only be used for BCD of homogeneous and heterogeneous remote sensing data respectively but also can input both homogeneous and heterogeneous remote sensing data for 2D or 3D BCD by relying on its bidirectional symmetric end-to-end network architecture. Moreover, from a unique perspective, we use image features that are stable in performance and less affected by radiation differences and temporal changes. We innovatively introduced the squeeze-and-excitation module to explicitly model the interdependence between feature channels so that the response between the feature channels is adaptively recalibrated to improve the information mining ability and detection accuracy of the model. As far as we know, this is the first proposed network architecture that can simultaneously use multi-source and multi-feature remote sensing data for 2D and 3D BCD. The experimental results in two 2D data sets and two challenging 3D data sets demonstrate that the promising performances of the squeeze-and-excitation W-Net outperform several traditional and state-of-the-art approaches. Moreover, both visual and quantitative analyses of the experimental results demonstrate competitive performance in the proposed network. This demonstrates that the proposed network and method are practical, physically justified, and have great potential application value in large-scale 2D and 3D BCD and qualitative and quantitative research.
Altitude on Cartographic Materials and Its Correction According to New Measurement Techniques
Authors: Maciuk Kamil , Apollo Michał , Mostowska Joanna , Lepeška Tomáš , Poklar Mojca , Noszczyk Tomasz , Kroh Pawel , Krawczyk Artur , Borowski Łukasz , Pavlovčič-Prešeren Polona
Published: January 2021

Determining the correct height of mountain peaks is vital for tourism, but it is also important as a reference point for devices equipped with GPS (Global Positioning System), e.g., watches or car navigation systems. The peak altitude data are part of geographic and geodetic information. As more modern technologies and equipment become available, their precisions should increase. However, verification of peak heights is usually only conducted for the highest, well-known mountains—lower peaks or mountain passes are rarely verified. Therefore, this study focuses on an investigation of 12 altitude points on a section of the longest and most famous touristic trail in Poland (the Main Beskid Trail), located in the Orava–Żywiec Beskids Mts (Mountains). The aim of this research is to measure and verify the heights of the 12 selected mountain peaks, in addition to evaluating the chosen methods based on the quality of the obtained data and determining their suitability and opportunities for use in further research. Measurements were obtained at the most specific height points—on the 12 highest points of the summits. This study compares two modern measurement techniques: the global navigation satellite system (GNSS) and light detection and ranging (LiDAR). The obtained results were later compared with those widely used on the internet and in printed materials (period covered: 1884–2015). This analysis demonstrates that lesser-known objects are rarely the subject of remeasurement and significant altitude errors may occur, primarily because the heights originated from a source in the past when modern methods were not available. Our findings indicate that the heights of the peaks presented in cartographic materials are inaccurate. The assumed heights should be corrected by direct measurements using modern techniques.
Volumetric Analysis of the Landslide in Abe Barek, Afghanistan Based on Nonlinear Mapping of Stereo Satellite Imagery-Derived DEMs
Authors: Atefi Mujeeb Rahman , Miura Hiroyuki
Published: January 2021

On 2 May 2014, a large-scale landslide in Abe Barek, Badakhshan, Afghanistan, produced extensive damage to the buildings and killed hundreds of people. Evaluations of the extent and the volume of the displaced materials are vital for post-disaster management activities. In this study, we present the applicability of a nonlinear geometric correction technique for decreasing the undesired registration errors between pre- and post-event digital elevation models (DEMs) generated from high-resolution stereo pair satellite imagery, identifying landslide affected areas, and quantifying the landslide volume from DEMs of difference (DoD) analysis. The nonlinear mapping method consists of shifting vector generation in subareas of the DEMs, consensus operations, and interpolation of the shifting vectors. The quality assessment confirmed that the method outperformed the simple DoD technique by eliminating a large-scale of geometric errors in an unaffected area. We estimated the volume of the landslide as 1.05 × 106 m3 from the DoD corrected by the nonlinear method, and discussed the relationship between the area and volume compared to those of the previous studies.
Distributed Sensing Network Enabled by High-Scattering MgO-Doped Optical Fibers for 3D Temperature Monitoring of Thermal Ablation in Liver Phantom
Authors: Beisenova Aidana , Issatayeva Aizhan , Ashikbayeva Zhannat , Jelbuldina Madina , Aitkulov Arman , Inglezakis Vassilis , Blanc Wilfried , Saccomandi Paola , Molardi Carlo , Tosi Daniele
Published: January 2021

Thermal ablation is achieved by delivering heat directly to tissue through a minimally invasive applicator. The therapy requires a temperature control between 50–100 °C since the mortality of the tumor is directly connected with the thermal dosimetry. Existing temperature monitoring techniques have limitations such as single-point monitoring, require costly equipment, and expose patients to X-ray radiation. Therefore, it is important to explore an alternative sensing solution, which can accurately monitor temperature over the whole ablated region. The work aims to propose a distributed fiber optic sensor as a potential candidate for this application due to the small size, high resolution, bio-compatibility, and temperature sensitivity of the optical fibers. The working principle is based on spatial multiplexing of optical fibers to achieve 3D temperature monitoring. The multiplexing is achieved by high-scattering, nanoparticle-doped fibers as sensing fibers, which are spatially separated by lower-scattering level of single-mode fibers. The setup, consisting of twelve sensing fibers, monitors tissue of 16 mm × 16 mm × 25 mm in size exposed to a gold nanoparticle-mediated microwave ablation. The results provide real-time 3D thermal maps of the whole ablated region with a high resolution. The setup allows for identification of the asymmetry in the temperature distribution over the tissue and adjustment of the applicator to follow the allowed temperature limits.
Integrating Cognitive Radio with Unmanned Aerial Vehicles: An Overview
Authors: Dias Santana Guilherme Marcel , Cristo Rogers Silva de , Lucas Jaquie Castelo Branco Kalinka Regina
Published: January 2021

Unmanned Aerial Vehicles (UAVs) demand technologies so they can not only fly autonomously, but also communicate with base stations, flight controllers, computers, devices, or even other UAVs. Still, UAVs usually operate within unlicensed spectrum bands, competing against the increasing number of mobile devices and other wireless networks. Combining UAVs with Cognitive Radio (CR) may increase their general communication performance, thus allowing them to execute missions where the conventional UAVs face limitations. CR provides a smart wireless communication which, instead of using a transmission frequency defined in the hardware, uses software transmission. CR smartly uses free transmission channels and/or chooses them according to application’s requirements. Moreover, CR is considered a key enabler for deploying technologies that require high connectivity, such as Smart Cities, 5G, Internet of Things (IoT), and the Internet of Flying Things (IoFT). This paper presents an overview on the field of CR for UAV communications and its state-of-the-art, testbed alternatives for real data experiments, as well as specifications to build a simple and low-cost testbed, and indicates key opportunities and future challenges in the field.