Trypodendron laeve Eggers w Polsce na tle wybranych aspektów morfologicznych i genetycznych drwalników (Trypodendron spp., Coleoptera, Curculionidae, Scolytinae)
Authors:   University of Agriculture in Krakow, Bilański Piotr 
Published: November 2021

In Poland, there are 4 species of the liypodendron genus: T lineaium Oliv., T domestkum L., T signature Fakir. and 7: laeve Egg. Trypodendron laeve is the leastknown of this group. Many factors had influence on the state of research on this species, including taxonomic aspects. Taking into account the unsatisfactory state of knowledge regarding the prevalence of T iaeve in Poland, as well as scarce information on the morphology of this species, research was undertaken to I) document the presence, including new sites, of T laeve in Poland and define, if possible, the habitat and trophic conditions that may affect its occurrence, as well as II) determinate suitability of biometric and genetic methods for correct identification of t laeve against the background of other ambrosia beetle species. Research on the occurrence of T laeve in Poland, was carried out on 143 areas located throughout the country, representing various environmental conditions, primarily such as species composition of tree stands, terrain, altitude (from 16 to 929 meters above sea level) and their location in relation to zoogeographic regions. The research material was obtained mainly using various types of traps for catching ambrosia beetles baited with pheromone. Only in a few cases when attacking the wood of trees, the imagines of ambrosia beetles were obtained without luring agents. The research was conducted in 2007-2016. From the insect individuals identified on the grounds of morphological traits as T lineatum, T laeve, T domesticum and T signatum, originating from selected locations in Poland, 3-11 specimens were collected, for which genetic analyses were performed based on the COI gene fragments obtained by PCR. The research included tests for following paramcter: s sequence similarity, phylogenetic, evolutionary divergence and genetic. structure. As a result of research on the occurrence of ambrosia beetles in Poland, a total of 44207 individuals belonging to four species were collected: T lineatutn, 7: laeve, T domesticum and T signatum, whose share was respectively: 49.2%, 31.4%; 19.1% and 0.3%. In Poland, 1: laeve's imagines were found in 124 out of 143 examined sites. The presence of L reeve has been documented for the first time in 14 zoogeographic regions. This species was commonly found on study areas located from 118 to 929 m above sea level. In Poland the tree species attacked by T Mate include Pinus sylvestris L. and Picea abies (L) H. Karst. In Poland, T laeve as a host plant prefers sylvestris and reaches the highest population densities in the stands of this species. The work presents the exact morphological characteristics of T laeve and indicates the most important features that distinguish it from the other Trypodendrun spp. occurring in Poland. It has also been shown that the best results in the determination of species of the liypodendron genus, regardless of their sex, can be obtained using phylogenetic analysis based on a fragment of the COI gene.