bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Comorbidity and Obesity on the Occurrence of Vascular Events in Obstructive Apnoea Treated with CPAP
Authors: Jurado-Robles Inmaculada , Jurado-Gámez Bernabé , Feu Collado Nuria , Molina-Luque Rafael , Molina-Recio Guillermo
Published: September 2024

Background: Obesity has increased cardiovascular morbidity and mortality. It is the leading risk factor for obstructive sleep apnoea (OSA). The relationship between obesity-OSA and vascular disease seems clear. There is no consensus on whether CPAP (continuous positive airway pressure) treatment prevents vascular events. Objective: The aim of this study was to determine the effect of comorbidity and obesity on the risk of vascular events in patients with OSA treated with CPAP. Method: This study was a prospective study of historical cohorts of adult patients with OSA and CPAP. The sample was 3017 patients. Descriptive, survival (Kaplan–Meier) and Cox regression analyses were performed, calculating crude and adjusted association relationships to explain the risk of vascular events. Results: A total of 1726 patients were obese, 782 were diabetics, and 1800 were hypertensive. The mean adherence was 6.2 (±1.8 h/day), and the mean follow-up time was 2603 days (±953.3). In the COX regression analysis, the event-related variables were baseline age (HR: 1.025: 1.012–1.037; p < 0.001), pre-treatment vascular event (HR; 2.530: 1.959–3.266; p < 0.001), hypertension (HR; 1.871: 1.187–2.672; p = 0.005) and abbreviated Charlson comorbidity index (HR; 1.289: 1.100–1.510; p = 0.002). Conclusions: The occurrence of vascular events in OSA patients on CPAP treatment is related to hypertension, having a vascular event before treatment, age at the start of CPAP use and abbreviated Charlson comorbidity index.
Akkermansia muciniphila as a Potential Guardian against Oral Health Diseases: A Narrative Review
Authors: Anderson Molly H. , Ait-Aissa Karima , Sahyoun Amal M. , Abidi Ammaar H. , Kassan Modar
Published: September 2024

The oral microbiome is a diverse ecosystem containing a community of symbiotic, commensal, and pathogenic microorganisms. One key microorganism linked to periodontal disease (PD) is Porphyromonas gingivalis (P. gingivalis), a Gram-negative anaerobic bacterium known to have several virulence factors that trigger inflammation and immune evasion. On the other hand, Akkermansia muciniphila (A. muciniphila), a symbiotic bacterium, has been recently shown to play an important role in mitigating inflammation and reducing periodontal damage. In vivo and in vitro studies have shown that A. muciniphila decreases inflammatory mediators and improves immune responses, suggesting its role in mitigating PD and related inflammatory systemic conditions such as diabetes, hypertension, and obesity. This review discusses the anti-inflammatory effects of A. muciniphila, its impact on periodontal health, and its potential role in managing systemic diseases. The overall aim is to elucidate how this bacterium might help reduce inflammation, improve oral health, and influence broader health outcomes.
Sex-Specific Adherence to the Mediterranean Diet in Obese Individuals
Authors: Di Renzo Laura , Gualtieri Paola , Frank Giulia , Cianci Rossella , Raffaelli Glauco , Peluso Daniele , Bigioni Giulia , De Lorenzo Antonino
Published: September 2024

Adherence to the Mediterranean diet (MedDiet) has long been associated with several health benefits, including a reduced risk of heart disease, diabetes, and obesity. The MedDiet is characterized by a high consumption of foods such as fruits, vegetables, whole grains, fish, and olive oil, along with a moderate intake of red meat and red wine with meals. Some studies report significant differences between men and women in susceptibility to obesity, with women at a higher prevalence of obesity than men. One unexplored aspect, however, concerns the sex difference in MedDiet adherence, which could be influenced by various factors, such as health perceptions, food preferences, and cultural influences. The aim of this study is to assess the effectiveness and impact of MedDiet adherence in men and women, with a focus on its influence on health and well-being, as well as its ability to promote sex equity in healthcare outcomes. Moreover, we aim to measure the overall health improvements in men and women participating in a MedDiet program, including changes in body composition and overall quality of life. This study highlights that the MedDiet is associated with more significant body weight loss in women, although their increase in MedDiet adherence was lower than in men. Trial registration: NCT01890070. Registered 24 June 2013.
Effects of Maltodextrin–Fructose Supplementation on Inflammatory Biomarkers and Lipidomic Profile Following Endurance Running: A Randomized Placebo-Controlled Cross-Over Trial
Authors: Righetti Stefano , Medoro Alessandro , Graziano Francesca , Mondazzi Luca , Martegani Serena , Chiappero Francesco , Casiraghi Elena , Petroni Paolo , Corbi Graziamaria , Pina Riccardo , Scapagnini Giovanni , Davinelli Sergio , Ricordi Camillo
Published: September 2024

Background: Managing metabolism for optimal training, performance, and recovery in medium-to-high-level endurance runners involves enhancing energy systems through strategic nutrient intake. Optimal carbohydrate intake before, during, and after endurance running can enhance glycogen stores and maintain optimal blood glucose levels, influencing various physiological responses and adaptations, including transitory post-endurance inflammation. This randomized trial investigates the impact of a high-dose 2:1 maltodextrin–fructose supplementation to medium-to-high-level endurance runners immediately before, during, and after a 15 km run at 90% VO2max intensity on post-exercise inflammatory stress. Methods: We evaluated inflammatory biomarkers and lipidomic profiles before the endurance tests and up to 24 h after. We focused on the effects of high-dose 2:1 maltodextrin–fructose supplementation on white blood cell count, neutrophil number, IL-6, cortisol, and CRP levels, as well as polyunsaturated fatty acids, ω-3 index, and AA/EPA ratio. Results: This supplementation significantly reduced inflammatory markers and metabolic stress. Additionally, it may enhance the post-activity increase in blood ω-3 fatty acid levels and reduce the increase in ω-6 levels, resulting in a lower trend of AA/EPA ratio at 24 h in the treated arm. Conclusions: Adequate carbohydrate supplementation may acutely mitigate inflammation during a one-hour endurance activity of moderate-to-high intensity. These effects could be beneficial for athletes engaging in frequent, high-intensity activities.
“One Health” Perspective on Prevalence of ESKAPE Pathogens in Africa: A Systematic Review and Meta-Analysis
Authors: Khasapane Ntelekwane George , Nkhebenyane Sebolelo Jane , Lekota Kgaugelo , Thekisoe Oriel , Ramatla Tsepo
Published: September 2024

The leading cause of hospital-acquired infections worldwide includes Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) infections. These bacteria are commonly isolated from clinical settings and linked to a number of potentially fatal diseases associated with hospitals. The objective of this study was to review the prevalence of ESKAPE pathogens in Africa. We gathered and systematically reviewed the literature concerning the prevalence of ESKAPE pathogens, published in the English language from January 2014 to February 2024, from three databases (PubMed, Web of Science and ScienceDirect). Our overall results revealed that S. aureus was the most prevalent species (79.5%), followed by A. baumannii (27.6%), K. pneumoniae (24.2%), Enterobacter spp. (20%), P. aeruginosa (9.0%), and E. faecium (5.1%). Moreover, stool samples had the highest Pooled Prevalence Estimates (PPEs) of 44.0%, followed by urine, nasal, and blood samples with 37.3%, 26.9%, and 22.9%, respectively. For the diagnostic method used to identify these ESKAPE pathogens, VITEK-MS had the highest PPE of 55.2%, followed by whole genome sequencing and PCR with 37.1% and 33.2%, respectively. The highest PPE of ESKAPE pathogens was recorded in West Africa with 77.3%, followed by Central/Middle Africa and East Africa with 43.5% and 25.1%, respectively. The overall PPE of ESKAPE pathogens from humans, animals, the environment (water, soil, and surfaces) and food sources was 35.8%, 37.3%, 47.7%, and 34.2%, respectively. Despite their prevalence in nosocomial settings, studies have shown that the ESKAPE pathogens may be isolated from a range of environmental reservoirs, including soil, dumping sites, beach sand, wastewater, food, and fish farms, among others. This wide source of ESKAPE pathogens substrates indicates the need for a multidisciplinary collaborative partnership for epidemiological studies and intervention efforts by the human, veterinary, and environmental health sectors in Africa.
Metagenomics Insight into Veterinary and Zoonotic Pathogens Identified in Urban Wetlands of Los Lagos, Chile
Authors: Opitz-Ríos Catherine , Burgos-Pacheco Alvaro , Paredes-Cárcamo Francisca , Campanini-Salinas Javier , Medina Daniel A.
Published: September 2024

Wetlands are ecosystems that are essential to ecological balance and biodiversity; nevertheless, human activity is a constant threat to them. Excess nutrients are caused by intensive livestock and agricultural operations, pollution, and population growth, which in turn leads to uncontrolled microbiological development. This impairment in water quality can constitute a risk to animal, human, and environmental health. To thoroughly characterize the microbial communities, shotgun metagenomics was used to characterize the taxonomic and functional pattern of microorganisms that inhabit urban wetlands in the Los Lagos Region of Chile. The main objective was to identify microorganisms of veterinary relevance, assess their potential antibiotic resistance, and characterize the main virulence mechanism. As expected, a high diversity of microorganisms was identified, including bacteria described as animal or human pathogens, such as Pasteurella multocida, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Also, a diverse repertory of antimicrobial-resistant genes (ARGs) was detected in metagenomic assembled sequences and inside the sequence of mobile genetic elements, genes that confer mainly resistance to beta-lactams, consistent with the families of antibiotics most used in Chile. In addition, a diverse collection of virulence mechanisms was also identified. Given the significance of the relationship between environmental, animal, and human health—a concept known as One Health—there is a need to establish molecular surveillance programs that monitor the environmental biohazard elements using molecular tools. This work is the first report of the presence of these harmful biological elements in urban wetlands subjected to anthropogenic pressure, located in the south of Chile.
Global Research Trends in the Links between Periodontal Disease and Cancer: A Bibliometric Analysis
Authors: Hu Suh-Woan , Yang Jaw-Ji , Lin Yuh-Yih
Published: September 2024

Both periodontal disease and cancer are prevalent conditions with significant impacts on individuals and society. Extensive research has suggested a potential link between these two diseases. This study conducted a bibliometric analysis using the Thomson Reuters Web of Science Core Collection database, focusing on publications from 2014 to 2023. The analysis included data extraction and examination of authors, affiliations, publication dates, journals, countries, citation counts, keywords, and the H-index. A total of 253 relevant articles were identified, showing an increasing trend in both publications and citations over the years. The analysis highlighted the most productive authors, institutions, and countries/regions, with Michaud DS and Abnet CC leading in the number of publications. Highly cited articles emphasized the role of specific oral microbiota, particularly F. nucleatum and P. gingivalis, in various cancers, suggesting their potential as diagnostic markers and therapeutic targets. Four key thematic clusters emerged from the keyword analysis: the broader health implications of periodontal disease, the microbiome’s role in carcinogenesis, inflammation, and specific bacteria in cancer, and epidemiological methods in studying the disease–cancer association. This bibliometric analysis underscores the growing interest in the connection between periodontal disease and cancer. Future research should adopt interdisciplinary approaches, focus on large-scale microbiome studies and longitudinal research to understand the systemic effects of periodontal disease, identify cancer-associated bacterial profiles, and investigate the molecular mechanisms of bacterial carcinogenesis. Additionally, public health interventions aimed at improving oral hygiene and reducing cancer risk factors are recommended.
Diagnostic Pathways of Leptospira spp. in Dogs with Kidney Injury
Authors: Hutu Ioan , Boldura Oana Maria , Luca Iasmina , Pasca Sorin Aurelian , Dragoescu Alina Andreea , Gros Radu Valentin , Lungu Bianca Cornelia , Călugăriță Andrei , Baltă Cornel , Mircu Călin , Stancu Adrian Constantin
Published: September 2024

Pathogenic Leptospira spp. causes leptospirosis in animals and humans globally, leading to systemic infections that can impact vital organs in affected animals. The purpose of this study was to evaluate kidney injury and to perform a retrospective analysis of leptospirosis infection in follow-up dog samples. The retrospective study collected epidemiological information obtained through paraclinical exams, immunohistochemistry (IHC), and molecular biology (qPCR) of cases from the Faculty of Veterinary Medicine from Timisoara between September 2016 and May 2023. No correlations were found between Leptospira infection and breed (p = 0.714), gender or castration status (p = 0.890), and anatomic pathology exam results (p = 0.608). Significant associations were found in cases with high levels of azotemia (p = 0.000) and immunological status (vaccinated vs. unvaccinated, p = 0.000), with the leptospirosis risk in unvaccinated animals calculated at OR = 85.500 (95%CI, 6.82–1071.26, p = 0.000). Retrospectively, leptospirosis was diagnosed in 27/65 cases (42%) using the IHC method, while the qPCR assay detected 29/65 cases (45%). This study demonstrates that qPCR is a robust and specific method for postmortem diagnosis of Leptospira spp. infection in dogs, offering higher specificity and reliability compared to traditional IHC methods, which showed 94.74% specificity in our study.
High-Risk HPV Detection in Paraffin-Embedded Tissue from Cervical Lesions
Authors: Almeida Micaela , Caeiro Vitor , Costa Diana , Silva Lara , Sousa Cíntia , Pestana Paula , Campelos Sofia , Vale João , Ramalhinho Ana Cristina , Fonseca-Moutinho José , Breitenfeld Luiza
Published: September 2024

Background: Human papillomavirus (HPV), a leading cause of cervical cancer, is present in most cases of the disease and ranks as the fourth most common cancer in women globally. Among the HPV types, fourteen (HPV 16/18/31/33/35/39/45/51/52/56/58/59/66/68) are recognized as high-risk (hrHPV), each with varying levels of oncogenic potential. Detecting and genotyping these hrHPV types in cervical lesions is crucial, requiring the development of new diagnostic methods. Methods: This study focuses on a retrospective analysis conducted on 44 women from the Cova da Beira Local Health Unit. We used the Anyplex™ II hrHPV Detection kit for hrHPV genotyping from paraffin-embedded cervical tissue samples. Results: hrHPV types were identified in 38 out of the 44 women. Genotyping revealed HPV-16 (55.3%), HPV-18/39/56/58/59 (5.3%), HPV-31 (21.1%), HPV-35 (7.9%), HPV-51/66 (2.6%), and HPV-52 (10.5%). Conclusions: This study demonstrates that the Anyplex™ II hrHPV Detection kit, originally designed for cervical cancer screening, is also effective for hrHPV genotyping in histological analyses. This methodology offers a simpler and more cost-effective approach for cervical cancer risk stratification. Its implementation in clinical practice could enhance the detection of hrHPV in cervical lesions, thereby contributing to more precise diagnoses and potentially more informed treatment strategies.
Bixin Combined with Metformin Ameliorates Insulin Resistance and Antioxidant Defenses in Obese Mice
Authors: Pinheiro Camila Graça , Motta Bruno Pereira , Oliveira Juliana Oriel , Cardoso Felipe Nunes , Figueiredo Ingrid Delbone , Machado Rachel Temperani Amaral , da Silva Patrícia Bento , Chorilli Marlus , Brunetti Iguatemy Lourenço , Baviera Amanda Martins
Published: September 2024

Bixin (C25H30O4; 394.51 g/mol) is the main apocarotenoid found in annatto seeds. It has a 25-carbon open chain structure with a methyl ester group and carboxylic acid. Bixin increases the expression of antioxidant enzymes, which may be interesting for counteracting oxidative stress. This study investigated whether bixin-rich annatto extract combined with metformin was able to improve the disturbances observed in high-fat diet (HFD)-induced obesity in mice, with an emphasis on markers of oxidative damage and antioxidant defenses. HFD-fed mice were treated for 8 weeks with metformin (50 mg/kg) plus bixin-rich annatto extract (5.5 and 11 mg/kg). This study assessed glucose tolerance, insulin sensitivity, lipid profile and paraoxonase 1 (PON-1) activity in plasma, fluorescent AGEs (advanced glycation end products), TBARSs (thiobarbituric acid-reactive substances), and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver and kidneys. Treatment with bixin plus metformin decreased body weight gain, improved insulin sensitivity, and decreased AGEs and TBARSs in the plasma, liver, and kidneys. Bixin plus metformin increased the activities of PON-1, SOD, CAT, and GSH-Px. Bixin combined with metformin improved the endogenous antioxidant defenses in the obese mice, showing that this combined therapy may have the potential to contrast the metabolic complications resulting from oxidative stress.
Inhibition of Nitric Oxide Synthesis Prevents the Effects of Intermittent Social Defeat on Cocaine-Induced Conditioned Place Preference in Male Mice
Authors: Martínez-Caballero María Ángeles , García-Pardo María Pilar , Calpe-López Claudia , Arenas María Carmen , Manzanedo Carmen , Aguilar María Asuncion
Published: September 2024

We have previously observed that mice exposed to social defeat stress are more sensitive to cocaine in the conditioned place preference (CPP) paradigm. In this context, it has been suggested that the nitric oxide (NO) pathway plays a role in the effects of stress. The present study evaluates the role of a neuronal NO synthase (nNOS) inhibitor (7-nitroindazole, 7-NI) in the short- and long-term behavioural effects of intermittent social defeat (ISD). Four groups of mice were employed for the study: a control group and three stressed groups, one treated with vehicle and two treated with 7-NI (7.25 or 12.5 mg/kg). After the last episode of defeat, mice were tested in the elevated plus maze (EPM), social interaction, object recognition and tail suspension tests. Three weeks later, mice were conditioned with cocaine (1 mg/kg). Stressed mice, irrespective of the treatment received, showed anxiety in the EPM, presented a deficit of social interaction and spent less time immobile in the tail suspension test. However, only stressed mice treated with vehicle developed CPP. Thus, although 7-NI did not modify the short-term behavioural effects of ISD, it prevented ISD-induced potentiation of the rewarding properties of cocaine in adulthood. These results support a specific role of nNOS in the effects of social stress on drug reward.
Transmission Dynamics and Novel Treatments of High Risk Carbapenem-Resistant Klebsiella pneumoniae: The Lens of One Health
Authors: Zhu Jiaying , Chen Taoyu , Ju Yanmin , Dai Jianjun , Zhuge Xiangkai
Published: September 2024

The rise of antibiotic resistance and the dwindling antimicrobial pipeline have emerged as significant threats to public health. The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a global threat, with limited options available for targeted therapy. The CRKP has experienced various changes and discoveries in recent years regarding its frequency, transmission traits, and mechanisms of resistance. In this comprehensive review, we present an in-depth analysis of the global epidemiology of K. pneumoniae, elucidate resistance mechanisms underlying its spread, explore evolutionary dynamics concerning carbapenem-resistant hypervirulent strains as well as KL64 strains of K. pneumoniae, and discuss recent therapeutic advancements and effective control strategies while providing insights into future directions. By going through up-to-date reports, we found that the ST11 KL64 CRKP subclone with high risk demonstrated significant potential for expansion and survival benefits, likely due to genetic influences. In addition, it should be noted that phage and nanoparticle treatments still pose significant risks for resistance development; hence, innovative infection prevention and control initiatives rooted in One Health principles are advocated as effective measures against K. pneumoniae transmission. In the future, further imperative research is warranted to comprehend bacterial resistance mechanisms by focusing particularly on microbiome studies’ application and implementation of the One Health strategy.
Assessing α-Bisabolol as a Transmucosal Permeation Enhancer of Buccal Local Anesthetics
Authors: do Couto Renê Oliveira , Thomaz Douglas Vieira , Duarte Maira Perez Ferreira , Lopez Renata Fonseca Vianna , Pedrazzi Vinícius , de Freitas Osvaldo , Tartaglia Gianluca Martino
Published: September 2024

Needle-free buccal anesthesia improves dental treatment outcomes for both patients and dentists. In this study, we report on an assessment of the enhancement effects of α-bisabolol on the in vitro transmucosal permeation of prilocaine hydrochloride (PCl) and lidocaine hydrochloride (LCl) from needleless buccal films. We also evaluated the mechanical properties of the film, which consisted of Methocel™ K100 LV as the film-forming polymer (3% m·m−1), PEG 400 as a cosolvent (15% m·m−1 based on drug loading), α-bisabolol (15 and 30% m·m−1 based on drug loading), and the drugs combined at a 1:1 ratio (15 mg·unit−1). The porcine esophageal epithelium was used as a membrane barrier, and artificial saliva was the release medium. After a 1 h experiment at 25 ± 2 °C, α-bisabolol significantly decreased, rather than enhanced, the permeation fluxes (five-fold), permeability coefficients (seven-fold), and retentions (two-fold) of both PCl and LCl through the epithelium, regardless of the concentration. Moreover, the resistance and flexibility of the films markedly decreased compared to those without α-bisabolol. Therefore, under the experimental conditions, using α-bisabolol as a buccal permeation enhancer for the hydrophilic local anesthetics PCl and LCl from buccal films is not feasible.
Exploring Italian Autochthonous Punica granatum L. Accessions: Pomological, Physicochemical, and Aromatic Investigations
Authors: Beghè Deborah , Cirlini Martina , Beneventi Elisa , Dall’Asta Chiara , Marchioni Ilaria , Petruccelli Raffaella
Published: September 2024

Autochthonous Italian pomegranate accessions are still underexplored, although they could be an important resource for fresh consumption, processing, and nutraceutical uses. Therefore, it is necessary to characterize the local germplasm to identify genotypes with desirable traits. In this study, six old Italian pomegranate landraces and a commercial cultivar (Dente di Cavallo) were investigated, evaluating their fruit pomological parameters, physicochemical (TSS, pH, TA, and color) characteristics, sugar content, and aromatic profiles (HeadSpace Solid-Phase MicroExtraction (HS-SPME)) coupled with Gas Chromatographyass Spectrometry (GC–MS) of pomegranate juices. Significant differences were observed in the size and weight of the seed and fruits (127.50–525.1 g), as well as the sugar content (100–133.6 gL−1), the sweetness (12.9–17.6 °Brix), and the aroma profiles. Over 56 volatile compounds, predominantly alcohols (56%), aldehydes (24%), and terpenes (9%), were simultaneously quantified. Large variability among the genotypes was also statistically confirmed. The results indicate a strong potential for commercial exploitation of this germplasm, both as fresh and processed fruit, and highlight its versatility for diverse applications. The genetic diversity of the autochthonous pomegranate accessions represents a precious heritage to be preserved and enhanced. This work represents a preliminary step toward a more comprehensive characterization and qualitative valorization of the Italian pomegranate germplasm.
Current Insights into Weak Seed Dormancy and Pre-Harvest Sprouting in Crop Species
Authors: Matilla Angel J.
Published: September 2024

During the domestication of crops, seed dormancy has been reduced or eliminated to encourage faster and more consistent germination. This alteration makes cultivated crops particularly vulnerable to pre-harvest sprouting, which occurs when mature crops are subjected to adverse environmental conditions, such as excessive rainfall or high humidity. Consequently, some seeds may bypass the normal dormancy period and begin to germinate while still attached to the mother plant before harvest. Grains affected by pre-harvest sprouting are characterized by increased levels of α-amylase activity, resulting in poor processing quality and immediate grain downgrading. In the agriculture industry, pre-harvest sprouting causes annual economic losses exceeding USD 1 billion worldwide. This premature germination is influenced by a complex interplay of genetic, biochemical, and molecular factors closely linked to environmental conditions like rainfall. However, the exact mechanism behind this process is still unclear. Unlike pre-harvest sprouting, vivipary refers to the germination process and the activation of α-amylase during the soft dough stage, when the grains are still immature. Mature seeds with reduced levels of ABA or impaired ABA signaling (weak dormancy) are more susceptible to pre-harvest sprouting. While high seed dormancy can enhance resistance to pre-harvest sprouting, it can lead to undesirable outcomes for most crops, such as non-uniform seedling establishment after sowing. Thus, resistance to pre-harvest sprouting is crucial to ensuring productivity and sustainability and is an agronomically important trait affecting yield and grain quality. On the other hand, seed color is linked to sprouting resistance; however, the genetic relationship between both characteristics remains unresolved. The identification of mitogen-activated protein kinase kinase-3 (MKK3) as the gene responsible for pre-harvest sprouting-1 (Phs-1) represents a significant advancement in our understanding of how sprouting in wheat is controlled at the molecular and genetic levels. In seed maturation, Viviparous-1 (Vp-1) plays a crucial role in managing pre-harvest sprouting by regulating seed maturation and inhibiting germination through the suppression of α-amylase and proteases. Vp-1 is a key player in ABA signaling and is essential for the activation of the seed maturation program. Mutants of Vp-1 exhibit an unpigmented aleurone cell layer and exhibit precocious germination due to decreased sensitivity to ABA. Recent research has also revealed that TaSRO-1 interacts with TaVp-1, contributing to the regulation of seed dormancy and resistance to pre-harvest sprouting in wheat. The goal of this review is to emphasize the latest research on pre-harvest sprouting in crops and to suggest possible directions for future studies.
Optimization of Ultrasonic-Assisted Extraction of Phenolic Compounds and Antioxidant Activity from Araticum Peel Using Response Surface Methodology
Authors: Andrade Amanda Cristina , Borsoi Felipe Tecchio , Saliba Ana Sofia Martelli Chaib , de Alencar Severino Matias , Pastore Glaucia Maria , Arruda Henrique Silvano
Published: September 2024

The peel represents a significant portion of the araticum fruit (about 40%), which becomes waste after its consumption or processing. Previous studies have shown that the araticum peel is rich in phenolic compounds; however, little is known about the ideal conditions for recovering these compounds. Therefore, response surface methodology, using a central composite rotatable design, was employed to optimize the extraction process to maximize the total phenolic compounds (TPCs) and enhance the Trolox equivalent antioxidant capacity (TEAC) from araticum peel. The variables optimized were ethanol concentration (EC; 20–80%, v/v), extraction time (ET; 5–45 min), and solid–solvent ratio (SSR; 10–100 mg/mL). Additionally, condensed tannins, antioxidant capacity against synthetic free radicals (TEAC and FRAP) and reactive oxygen species (ROS), and the phenolic compounds profile, were evaluated. Optimum extraction conditions were 50% (v/v) ethanol concentration, 5 min of extraction time, and 10 mg/mL solid–solvent ratio. Under these conditions, experimental TPCs and TEAC values were 70.16 mg GAE/g dw and 667.22 µmol TE/g dw, respectively, comparable with predicted models (68.47 mg GAE/g dw for TPCs and 677.04 µmol TE/g dw for TEAC). A high condensed tannins content (76.49 mg CE/g dw) was also observed and 12 phenolic compounds were identified, predominantly flavonoids (97.77%), including procyanidin B2, epicatechin, and catechin as the major compounds. Moreover, a potent antioxidant activity was observed against synthetic free radicals and ROS, especially in scavenging peroxyl and hydroxyl radicals. From this study, we obtained the ideal conditions for recovering phenolic compounds from araticum peel using a simple, fast, sustainable, and effective method, offering a promising opportunity for the management of this plant byproduct.
Genome-Wide Analysis of Fruit Color and Carotenoid Content in Capsicum Core Collection
Authors: Ro Nayoung , Oh Hyeonseok , Ko Ho-Cheol , Yi Jungyoon , Na Young-Wang , Haile Mesfin
Published: September 2024

This study investigated carotenoid content and fruit color variation in 306 pepper accessions from diverse Capsicum species. Red-fruited accessions were predominant (245 accessions), followed by orange (35) and yellow (20). Carotenoid profiles varied significantly across accessions, with capsanthin showing the highest mean concentration (239.12 μg/g), followed by β-cryptoxanthin (63.70 μg/g) and zeaxanthin (63.25 μg/g). Total carotenoid content ranged from 7.09 to 2566.67 μg/g, emphasizing the diversity within the dataset. Correlation analysis revealed complex relationships between carotenoids, with strong positive correlations observed between total carotenoids and capsanthin (r = 0.94 ***), β-cryptoxanthin (r = 0.87 ***), and zeaxanthin (r = 0.84 ***). Principal component analysis (PCA) identified two distinct carotenoid groups, accounting for 67.6% of the total variance. A genome-wide association study (GWAS) identified 91 significant single nucleotide polymorphisms (SNPs) associated with fruit color (15 SNPs) and carotenoid content (76 SNPs). These SNPs were distributed across all chromosomes, with varying numbers on each. Among individual carotenoids, α-carotene was associated with 28 SNPs, while other carotenoids showed different numbers of associated SNPs. Candidate genes encoding diverse proteins were identified near significant SNPs, potentially contributing to fruit color variation and carotenoid accumulation. These included pentatricopeptide repeat-containing proteins, mitochondrial proton/calcium exchangers, E3 ubiquitin-protein ligase SINAT2, histone–lysine N-methyltransferase, sucrose synthase, and various enzymes involved in metabolic processes. Seven SNPs exhibited pleiotropic effects on multiple carotenoids, particularly β-cryptoxanthin and capsanthin. The findings of this study provide insights into the genetic architecture of carotenoid biosynthesis and fruit color in peppers, offering valuable resources for targeted breeding programs aimed at enhancing the nutritional and sensory attributes of pepper varieties.
Genome-Wide Identification and Characterization of Alternative Oxidase (AOX) Genes in Foxtail Millet (Setaria italica): Insights into Their Abiotic Stress Response
Authors: Zhang Hui , Luo Yidan , Wang Yujing , Zhao Juan , Wang Yueyue , Li Yajun , Pu Yihao , Wang Xingchun , Ren Xuemei , Zhao Bo
Published: September 2024

Alternative oxidase (AOX) serves as a critical terminal oxidase within the plant respiratory pathway, playing a significant role in cellular responses to various stresses. Foxtail millet (Setaria italica), a crop extensively cultivated across Asia, is renowned for its remarkable tolerance to abiotic stresses and minimal requirement for fertilizer. In this study, we conducted a comprehensive genome-wide identification of AOX genes in foxtail millet genome, discovering a total of five SiAOX genes. Phylogenetic analysis categorized these SiAOX members into two subgroups. Prediction of cis-elements within the promoter regions, coupled with co-expression network analysis, intimated that SiAOX proteins are likely involved in the plant’s adaptive response to abiotic stresses. Employing RNA sequencing (RNA-seq) and real-time quantitative PCR (RT-qPCR), we scrutinized the expression patterns of the SiAOX genes across a variety of tissues and under multiple abiotic stress conditions. Specifically, our analysis uncovered that SiAOX1, SiAOX2, SiAOX4, and SiAOX5 display distinct tissue-specific expression profiles. Furthermore, SiAOX2, SiAOX3, SiAOX4, and SiAOX5 exhibit responsive expression patterns under abiotic stress conditions, with significant differences in expression levels observed between the shoot and root tissues of foxtail millet seedlings. Haplotype analysis of SiAOX4 and SiAOX5 revealed that these genes are in linkage disequilibrium, with Hap_2 being the superior haplotype for both, potentially conferring enhanced cold stress tolerance in the cultivar group. These findings suggest that both SiAOX4 and SiAOX5 may be targeted for selection in future breeding programs aimed at improving foxtail millet’s resilience to cold stress.
Preparation of N-Halamine Gelatin Sponge and Its Application in the Treatment of Skin Infection
Authors: Zhu Jiahao , Xue Jiageng , Qin Huaiying , Wang Yiqing , Wang Yefan , Cheng Yidan , Ma Yingxia , Zhang Xiaoyun , Gong Chenliang , Zhao Guanghui
Published: September 2024

Nowadays, there has been an increasing research interest into N-halamine compounds due to their wide antimicrobial properties and no drug resistance. Most of the research mainly focuses on small molecular N-halamines, while few studies are on macromolecule N-halamines. In this work, antibacterial N-halamine polymer materials based on proteins (GS-Cl) were synthesized with an antibacterial component of oxidative chlorine, a support component of a gelatin sponge. After carrying out systematic characterization, the GS-Cls exhibited well-defined porous morphology and had a high efficiency in the killing of Gram-positive bacteria (E. coli) and Gram-negative bacteria (S. aureus). The loading of oxidative chlorine (Cl+%) could be controlled by changing the NaClO concentrations and chlorination times. The biocompatibility was confirmed as well. In vivo experiments suggested that the GS-Cl sample could effectively promote the healing of skin wounds in mice E. coli and S. aureus infection models. These studies show that proteins can be chlorinated and endowed with antimicrobial properties, which has great application potential in the treatment of bacteria-infected wounds.
Characterization of Exopolysaccharides Isolated from Donkey Milk and Its Biological Safety for Skincare Applications
Authors: La Torre Chiara , Plastina Pierluigi , Abrego-Guandique Diana Marisol , Caputo Paolino , Oliviero Rossi Cesare , Saraceno Giorgia Francesca , Caroleo Maria Cristina , Cione Erika , Fazio Alessia
Published: September 2024

Kefiran is a heteropolysaccharide that is considered a postbiotic and is obtained by kefir grains fermented in cow’s milk, while little is known about the donkey milk (DM) variety. Postbiotics are recognised as having important human health benefits that are very similar to probiotics but without the negative effects associated with their ingestion. Donkey is a monogastric animal, as are humans, and when used as an alternative food for infants who suffer from cow milk protein allergies, DM could therefore display more biocompatibility. In this study, the DM kefiran was extracted by ultrasound from kefir grains cultured in donkey milk and fully characterized for its structural and physicochemical properties by Fourier-transform infrared spectroscopy (FT-IR), High-Performance Liquid Chromatography- Refractive Index (HPLC-RI), Scanning electron microscope (SEM), Differential Scanning Calorimeters (DSC) and rheological analyses. In addition, tests were conducted on keratinocytes cell lines and human red blood cells to assess the nontoxicity and haemolysis degree of the polymer. The extraction yield of the DM kefiran was 6.5 ± 0.15%. The FT-IR analysis confirmed the structure of the polysaccharide by showing that the stretching of the C-O-C and C-O bonds in the ring, which formed two bands at 1157 and 1071 cm−1, respectively, and the anomeric band at 896 cm−1 indicates the β configuration and vibrational modes of glucose and galactose. Results were confirmed by HPLC-RI analysis indicating that the ratio glucose/galactose was 1:0.87. Furthermore, the SEM analysis showed a porous and homogeneous structure. The rheological analysis confirmed the pseudoplastic nature of the polymer, while the DSC analysis highlighted excellent thermal resistance (324 °C). Finally, DM kefiran was revealed to have biologically acceptable toxicity, showing a haemolytic activity of less than 2% when using fresh human red blood cells and showing no cytotoxicity on human keratinocytes. Therefore, kefiran obtained by DM shows an excellent biocompatibility, establishing it as a promising polymer for bioengineering human tissue for regenerative applications.
How Important Are Optimism and Coping Strategies for Mental Health? Effect in Reducing Depression in Young People
Authors: Palacios-Delgado Jorge , Acosta-Beltrán Delia Brenda , Acevedo-Ibarra Jessica Noemí
Published: September 2024

Depression is one of the most common disorders worldwide, including in Mexico. Engagement coping and optimism promote mental health, so understanding the mediation of optimism between coping strategies with depression helps to identify the psychological resources that young people can use to mitigate depression. This study aims to test the direct and indirect effects of four coping strategies through three optimism factors on affective depression in a mediator path model in young people. In a cross-sectional study, 848 young Mexican people completed the evaluation instruments of the Trait Depression Inventory, Mexican Optimism Scale (MOS), and Coping Strategies (CSI-SF). Moderate mediation analysis was performed. Optimism and coping strategies significantly predict depression. Furthermore, optimism mediated the association between problem-focused engagement coping and depressive symptoms. Problem- and emotion-focused coping and optimism protect young people from depression. Likewise, young people with greater optimism use problem-focused engagement coping, which reduces depressive symptoms. Psychosocial interventions that promote positive outlook, affective resources, and hope combined with engagement coping strategies are suggested for the management of depression.
Hospitable Education—Interreligious Education Revisited
Authors: Avest Ina ter
Published: September 2024

In the past, the Netherlands took on the form of a pillarized society, with three ‘pillars’ (Catholic, Protestant and liberal) ‘living apart together’. Each pillar came with its own education system, health care, and newspapers. In the 1980s, a fourth ‘pillar’ was added: the Islamic pillar. During the same period at the end of the 20th century, a model was developed at one of the country’s primary schools—the Juliana van Stolberg school—for the inclusive interreligious education of all pupils, irrespective of the (religious or secular) life orientation of their parents. This innovative educational process became the object of research. Literature reviews on (inter)religious education were complemented by qualitative interviews with the school’s principal and supplemented with historical research of the school’s filing cabinet kept in the city archives. I conclude that by revisiting the process and the developed model of inclusive interreligious education, its implementation in all schools is possible, provided that the latest insights are taken into account about leadership, biblical and qur’anic hermeneutics and the position of parents in the pedagogic civil society. For such an adapted model, I introduce the concept of hospitable education—hospitality as a competence, which connects knowledge, affective attitudes and skills.
Comparison of Soil Water Content from SCATSAR-SWI and Cosmic Ray Neutron Sensing at Four Agricultural Sites in Northern Italy: Insights from Spatial Variability and Representativeness
Authors: Emamalizadeh Sadra , Pirola Alessandro , Alessandrini Cinzia , Balenzano Anna , Baroni Gabriele
Published: September 2024

Monitoring soil water content (SWC) is vital for various applications, particularly in agriculture. This study compares SWC estimated by means of SCATSAR-SWI remote sensing (RS) at different depths (T-values) with Cosmic Ray Neutron Sensing (CRNS) across four agricultural sites in northern Italy. Additionally, it examines the spatial mismatch and representativeness of SWC products’ footprints based on different factors within the following areas: the Normalized Difference Vegetation Index (NDVI), soil properties (sand, silt, clay, Soil Organic Carbon (SOC)), and irrigation information. The results reveal that RS-derived SWC, particularly at T = 2 depth, exhibits moderate positive linear correlation (mean Pearson correlation coefficient, R = 0.6) and a mean unbiased Root–Mean–Square Difference (ubRMSD) of 14.90%SR. However, lower agreement is observed during summer and autumn, attributed to factors such as high biomass growth. Sites with less variation in vegetation and soil properties within RS pixels rank better in comparing SWC products. Although a weak correlation (mean R = 0.35) exists between median NDVI differences of footprints and disparities in SWC product performance metrics, the influence of vegetation greenness on the results is clearly identified. Additionally, RS pixels with a lower percentage of sand and SOC and silt loam soil type correlate to decreased agreement between SWC products. Finally, localized irrigation practices also partially explain some differences in the SWC products. Overall, the results highlight how RS pixel variability of the different factors can explain differences between SWC products and how this information should be considered when selecting optimal ground-based measurement locations for remote sensing comparison.
Identifying Determinants of Spatiotemporal Disparities in Ecological Quality of Mongolian Plateau
Authors: Wang Zhengtong , Song Yongze , Zhang Zehua , Lin Gang , Luo Peng , Zhang Xueyuan , Chai Zhengyuan
Published: September 2024

Vegetation quality is crucial for maintaining ecological health, and remote sensing techniques offer precise assessments of vegetation’s environmental quality. Although existing indicators and remote sensing approaches provide extensive spatial coverage, challenges remain in effectively integrating diverse indicators for a comprehensive evaluation. This study introduces a comprehensive ecological quality index (EQI) to assess vegetation quality on the Mongolian Plateau from 2001 to 2020 and to identify the determinants of EQI variations over space and time. We developed the EQI using remotely sensed normalized difference vegetation index (NDVI) data and the net primary productivity (NPP). Our analysis revealed distinct spatial patterns, with high ecological quality concentrated in northern Mongolia and eastern Inner Mongolia. Temporal fluctuations, indicative of ecological shifts, were primarily observed in eastern Mongolia and specific zones of Inner Mongolia. We employed a Geographically Optimal Zones-based Heterogeneity (GOZH) model to analyze the spatial scales and interactions influencing EQI patterns. This study found that precipitation, with an Omega value of 0.770, was the dominant factor affecting the EQI, particularly at spatial scales of 40–50 km. The GOZH model provided deeper insights into the spatial determinants of the EQI compared with previous models, highlighting the importance of climatic variables and their interactions in driving ecological quality. This research enhanced our understanding of vegetation quality dynamics and established a foundation for ecosystem conservation and informed management strategies, emphasizing the critical role of climate, especially precipitation, in shaping ecological landscapes.
Multilevel Geometric Feature Embedding in Transformer Network for ALS Point Cloud Semantic Segmentation
Authors: Liang Zhuanxin , Lai Xudong
Published: September 2024

Effective semantic segmentation of Airborne Laser Scanning (ALS) point clouds is a crucial field of study and influences subsequent point cloud application tasks. Transformer networks have made significant progress in 2D/3D computer vision tasks, exhibiting superior performance. We propose a multilevel geometric feature embedding transformer network (MGFE-T), which aims to fully utilize the three-dimensional structural information carried by point clouds and enhance transformer performance in ALS point cloud semantic segmentation. In the encoding stage, compute the geometric features surrounding tee sampling points at each layer and embed them into the transformer workflow. To ensure that the receptive field of the self-attention mechanism and the geometric computation domain can maintain a consistent scale at each layer, we propose a fixed-radius dilated KNN (FR-DKNN) search method to address the limitation of traditional KNN search methods in considering domain radius. In the decoding stage, we aggregate prediction deviations at each level into a unified loss value, enabling multilevel supervision to improve the network’s feature learning ability at different levels. The MGFE-T network can predict the class label of each point in an end-to-end manner. Experiments were conducted on three widely used benchmark datasets. The results indicate that the MGFE-T network achieves superior OA and mF1 scores on the LASDU and DFC2019 datasets and performs well on the ISPRS dataset with imbalanced classes.