bluuebunny/crossref_metadata_2025 huggingface dataset:
Ajania pacifica (Nakai) K. Bremer and Humphries Extract Limits MYC Expression to Induce Apoptosis in Diffuse Large B Cell Lymphoma
Authors: Woo Ye-Rin , Kwon Chan-Seong , Lee Ji-Eun , Jeon Byeol-Eun , Kim Tae-Jin , Choo Joy , Seo Young-Seob , Kim Sang-Woo
Published: May 2024

The proto-oncogene MYC is frequently dysregulated in patients with diffuse large B-cell lymphoma (DLBCL) and plays a critical role in disease progression. To improve the clinical outcomes of patients with DLBCL, the development of strategies to target MYC is crucial. The use of medicinal plants for developing anticancer drugs has garnered considerable attention owing to their diverse mechanisms of action. In this study, 100 plant extracts of flora from the Republic of Korea were screened to search for novel agents with anti-DLBCL effects. Among them, Ajania pacifica (Nakai) K. Bremer and Humphries extract (APKH) efficiently suppressed the survival of DLBCL cells, while showing minimal toxicity toward normal murine bone marrow cells. APKH suppressed the expression of anti-apoptotic BCL2 family members, causing an imbalance between the pro-apoptotic and anti-apoptotic BCL2 members. This disrupted mitochondrial membrane potential, cytochrome c release, and pro-caspase-3 activation and eventually led to DLBCL cell death. Importantly, MYC expression was markedly downregulated by APKH and ectopic expression of MYC in DLBCL cells abolished the pro-apoptotic effects of APKH. These results demonstrate that APKH exerts anti-DLBCL effects by inhibiting MYC expression. Moreover, when combined with doxorubicin, an essential component of the CHOP regimen (cyclophosphamide, doxorubicin, vincristine, and prednisone), APKH synergistically enhanced the therapeutic effect of doxorubicin. This indicates that APKH may overcome drug resistance, which is common in patients with refractory/relapsed DLBCL. To identify compounds with anti-DLBCL activities in APKH, the chemical profile analysis of APKH was performed using UPLC-QTOF/MSe analysis and assessed for its anticancer activity. Based on the UPLC-QTOF/MSe chemical profiling, it is conceivable that APKH may serve as a novel agent targeting MYC and sensitizing drug-resistant DLBCL cells to CHOP chemotherapy. Further studies to elucidate how the compounds in APKH exert tumor-suppressive role in DLBCL are warranted.
CDDO, an Anti-Inflammatory and Antioxidant Compound, Attenuates Vasospasm and Neuronal Cell Apoptosis in Rats Subjected to Experimental Subarachnoid Hemorrhage
Authors: Winardi William , Lo Yun-Ping , Tsai Hung-Pei , Huang Yu-Hua , Tseng Tzu-Ting , Chung Chia-Li
Published: May 2024

Subarachnoid hemorrhage (SAH) is a type of stroke caused by bleeding into the subarachnoid space. SAH is a medical emergency and requires prompt treatment to prevent complications such as seizures, stroke, or other brain damage. Treatment options may include surgery, medication, or a combination of both. 2-Cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO), a compound with anti-inflammatory and antioxidant properties, is currently being investigated as a potential treatment for various diseases, including chronic kidney disease and pulmonary arterial hypertension. In this study, the effects of CDDO on rats subjected to SAH were evaluated. Male Sprague-Dawley rats were divided into four groups (n = 6/group): (1) control group, (2) SAH group, (3) SAH + low-dose CDDO (10 mg/kg injected into the subarachnoid space at 24 h after SAH) group, and (4) SAH + high-dose CDDO (20 mg/kg) group. CDDO improved SAH-induced poor neurological outcomes and reduced vasospasm in the basal artery following SAH. It also decreased the SAH-induced expression of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6 in both the cerebrospinal fluid and serum samples as determined by ELISA. A Western blot analysis confirmed an increase in the p-NF-κB protein level after SAH, but it was significantly decreased with CDDO intervention. Immunofluorescence staining highlighted the proliferation of microglia and astrocytes as well as apoptosis of the neuronal cells after SAH, and treatment with CDDO markedly reduced the proliferation of these glial cells and apoptosis of the neuronal cells. The early administration of CDDO after SAH may effectively mitigate neuronal apoptosis and vasospasm by suppressing inflammation.
The Emerging Role of Human Gut Bacteria Extracellular Vesicles in Mental Disorders and Developing New Pharmaceuticals
Authors: Louka Effrosyni , Koumandou Vassiliki Lila
Published: May 2024

In recent years, further evidence has emerged regarding the involvement of extracellular vesicles in various human physiopathological conditions such as Alzheimer’s disease, Parkinson’s disease, irritable bowel syndrome, and mental disorders. The biogenesis and cargo of such vesicles may reveal their impact on human health nd disease and set the underpinnings for the development of novel chemical compounds and pharmaceuticals. In this review, we examine the link between bacteria-derived exosomes in the gastrointestinal tract and mental disorders, such as depression and anxiety disorders. Crucially, we focus on whether changes in the gut environment affect the human mental state or the other way around. Furthermore, the possibility of handling bacteria-derived exosomes as vectors of chemicals to treat such conditions is examined.
A Perfect Storm: The Convergence of Aging, Human Immunodeficiency Virus Infection, and Inflammasome Dysregulation
Authors: Thirugnanam Siva , Rout Namita
Published: May 2024

The emergence of combination antiretroviral therapy (cART) has greatly transformed the life expectancy of people living with HIV (PWH). Today, over 76% of the individuals with HIV have access to this life-saving therapy. However, this progress has come with a new challenge: an increase in age-related non-AIDS conditions among patients with HIV. These conditions manifest earlier in PWH than in uninfected individuals, accelerating the aging process. Like PWH, the uninfected aging population experiences immunosenescence marked by an increased proinflammatory environment. This phenomenon is linked to chronic inflammation, driven in part by cellular structures called inflammasomes. Inflammatory signaling pathways activated by HIV-1 infection play a key role in inflammasome formation, suggesting a crucial link between HIV and a chronic inflammatory state. This review outlines the inflammatory processes triggered by HIV-1 infection and aging, with a focus on the inflammasomes. This review also explores current research regarding inflammasomes and potential strategies for targeting inflammasomes to mitigate inflammation. Further research on inflammasome signaling presents a unique opportunity to develop targeted interventions and innovative therapeutic modalities for combating HIV and aging-associated inflammatory processes.
Genetic Susceptibility in Endothelial Injury Syndromes after Hematopoietic Cell Transplantation and Other Cellular Therapies: Climbing a Steep Hill
Authors: Evangelidis Paschalis , Evangelidis Nikolaos , Kalmoukos Panagiotis , Kourti Maria , Tragiannidis Athanasios , Gavriilaki Eleni
Published: May 2024

Hematopoietic stem cell transplantation (HSCT) remains a cornerstone in the management of patients with hematological malignancies. Endothelial injury syndromes, such as HSCT-associated thrombotic microangiopathy (HSCT-TMA), veno-occlusive disease/sinusoidal obstruction syndrome (SOS/VOD), and capillary leak syndrome (CLS), constitute complications after HSCT. Moreover, endothelial damage is prevalent after immunotherapy with chimeric antigen receptor-T (CAR-T) and can be manifested with cytokine release syndrome (CRS) or immune effector cell-associated neurotoxicity syndrome (ICANS). Our literature review aims to investigate the genetic susceptibility in endothelial injury syndromes after HSCT and CAR-T cell therapy. Variations in complement pathway- and endothelial function-related genes have been associated with the development of HSCT-TMA. In these genes, CFHR5, CFHR1, CFHR3, CFI, ADAMTS13, CFB, C3, C4, C5, and MASP1 are included. Thus, patients with these variations might have a predisposition to complement activation, which is also exaggerated by other factors (such as acute graft-versus-host disease, infections, and calcineurin inhibitors). Few studies have examined the genetic susceptibility to SOS/VOD syndrome, and the implicated genes include CFH, methylenetetrahydrofolate reductase, and heparinase. Finally, specific mutations have been associated with the onset of CRS (PFKFB4, CX3CR1) and ICANS (PPM1D, DNMT3A, TE2, ASXL1). More research is essential in this field to achieve better outcomes for our patients.
The Biological Impact of Some Phosphonic and Phosphinic Acid Derivatives on Human Osteosarcoma
Authors: Khaled Zakzak , Ilia Gheorghe , Watz Claudia , Macașoi Ioana , Drăghici George , Simulescu Vasile , Merghes Petru Eugen , Varan Narcis Ion , Dehelean Cristina Adriana , Vlaia Lavinia , Sima Laurențiu
Published: May 2024

Osteosarcoma malignancy currently represents a major health problem; therefore, the need for new therapy approaches is of great interest. In this regard, the current study aims to evaluate the anti-neoplastic potential of a newly developed phosphinic acid derivative (2-carboxyethylphenylphosphinic acid) and, subsequently, to outline its pharmaco-toxicological profile by employing two different in vitro human cell cultures (keratinocytes—HaCaT—and osteosarcoma SAOS-2 cells), employing different techniques (MTT assay, cell morphology assessment, LDH assay, Hoechst staining and RT-PCR). Additionally, the results obtained are compared with three commercially available phosphorus-containing compounds (P1, P2, P3). The results recorded for the newly developed compound (P4) revealed good biocompatibility (cell viability of 77%) when concentrations up to 5 mM were used on HaCaT cells for 24 h. Also, the HaCaT cultures showed no significant morphological alterations or gene modulation, thus achieving a biosafety profile even superior to some of the commercial products tested herein. Moreover, in terms of anti-osteosarcoma activity, 2-carboxyethylphenylphosphinic acid expressed promising activity on SAOS-2 monolayers, the cells showing viability of only 55%, as well as apoptosis features and important gene expression modulation, especially Bid downregulation. Therefore, the newly developed compound should be considered a promising candidate for further in vitro and in vivo research related to osteosarcoma therapy.
Contribution of Endothelial Dysfunction to Cancer Susceptibility and Progression: A Comprehensive Narrative Review on the Genetic Risk Component
Authors: de Melo Inês Guerra , Tavares Valéria , Pereira Deolinda , Medeiros Rui
Published: May 2024

Venous thromboembolism (VTE) is a challenging clinical obstacle in oncological settings, marked by elevated incidence rates and resulting morbidity and mortality. In the context of cancer-associated thrombosis (CAT), endothelial dysfunction (ED) plays a crucial role in promoting a pro-thrombotic environment as endothelial cells lose their ability to regulate blood flow and coagulation. Moreover, emerging research suggests that this disorder may not only contribute to CAT but also impact tumorigenesis itself. Indeed, a dysfunctional endothelium may promote resistance to therapy and favour tumour progression and dissemination. While extensive research has elucidated the multifaceted mechanisms of ED pathogenesis, the genetic component remains a focal point of investigation. This comprehensive narrative review thus delves into the genetic landscape of ED and its potential ramifications on cancer progression. A thorough examination of genetic variants, specifically polymorphisms, within key genes involved in ED pathogenesis, namely eNOS, EDN1, ACE, AGT, F2, SELP, SELE, VWF, ICAM1, and VCAM1, was conducted. Overall, these polymorphisms seem to play a context-dependent role, exerting both oncogenic and tumour suppressor effects depending on the tumour and other environmental factors. In-depth studies are needed to uncover the mechanisms connecting these DNA variations to the pathogenesis of malignant diseases.
A Review of Investigations and Applications of Biocides in Nanomaterials and Nanotechnologies
Authors: Issayeva Assem , Sharipova Altynay , Aidarova Saule , Madybekova Galiya , Katona Jaroslav , Turganbay Seitzhan , Miller Reinhard
Published: May 2024

In recent years, the development of nanomaterials with biocidal properties has received considerable attention due to their potential applications in various industries, including food, medicine, and cultural heritage preservation. The growing demand for coatings with antibacterial properties has sparked interest from industrial sectors in exploring the incorporation of biocides into these materials. Coatings are prone to microbial growth, which can cause damage such as cracking, discoloration, and staining. To combat these problems, the integration of biocides into coatings is a crucial strategy. Biocide-embedded nanomaterials offer numerous advantages, including high efficiency in small quantities, ease of application, good chemical stability, low toxicity, and non-bioaccumulation. Encapsulated nanobiocides are particularly attractive to the agro-industry, because they can be less toxic than traditional biocides while still effectively controlling microbial contamination. To fully exploit the benefits of nanobiocides, future research should focus on optimizing their synthesis, formulation, and delivery methods. The purpose of this review is to summarize the current status of biocide nanomaterials, discuss potential future research directions, and highlight research methods, the development of new forms of nanomaterials, and studies of their physico-chemical properties. Biocide nanocapsules of DCOIT (4,5-Dichloro-2-octyl-2H-isothiazol-3-one) are chosen as an example to illustrate the research pathways.
Trends in Early-Stage Cervical Cancer Management in the US: A National Cancer Database Analysis
Authors: Wolf Jennifer , Wu Yiyuan , Hayek Judy , Zhang Qi , Alagkiozidis Ioannis
Published: May 2024

The Laparoscopic Approach to Cervical Cancer (LACC) trial was published in 2018 and demonstrated that minimally invasive surgery (MIS) yields inferior survival outcomes in early-stage cervical cancer compared to open surgery. This study investigates how the results of the LACC trial have impacted the selection of the primary treatment modality and adjuvant radiation utilization in early-stage cervical cancer. Using the National Cancer Database (NCDB), we compared patients with stage IA2-IB1 cervical cancer before (1/2016–12/2017) and after (1/2019–12/2020) the LACC trial. A total of 7930 patients were included: 4609 before and 3321 after the LACC trial. There was a decline in MIS usage from 67% pre-LACC to 35% thereafter (p < 0.001). In both the pre- and post-LACC periods, patients undergoing radical MIS more frequently had small volume disease (pre-LACC tumors ≤ 2 cm, 48% MIS vs. 41% open, p = 0.023; post-LACC stage IA2, 22% vs. 15%, p = 0.002). Pre-LACC, MIS radical hysterectomy was associated with White race (82% vs. 77%, p = 0.001) and private insurance (63% vs. 54%, p = 0.004), while there was no difference in socioeconomic factors in the post-LACC period. Although the proportion of patients treated with primary chemoradiation remained stable, the post-LACC cohort had a younger median age (52.47 vs. 56.37, p = 0.005) and more microscopic disease cases (13% vs. 5.4%, p = 0.002). There was no difference in the rate of radiation after radical hysterectomy before and after the trial (26% vs. 24%, p = 0.3). Conclusions: Post-LACC, patients were less likely to undergo MIS but received adjuvant radiation at similar rates, and primary chemoradiation patients were younger and more likely to have microscopic disease.
Pharmacy Workload in Clinical Trial Management: A Preliminary Complexity Assessment Tool for Sponsored Oncology and Haematology Trials
Authors: Gasperoni Lorenzo , Masini Carla , Toscano Giada , Cafaro Alessandro , Zani Chiara , Andrianò Cristina , Silimbani Paolo , Donati Caterina , Bortolin Giorgia , Cecco Sara
Published: May 2024

Investigational drug services need to be organised in a structured approach, especially for sites with a large number of ongoing clinical trials. The aim of this study was to develop a tool to assess the complexity of pharmacy involvement in a sponsored oncology clinical trial. Categorisation into ordinal complexity categories was used to assess the complexity of the clinical trials for consistent pharmacy grant applications. The 15 items of the tool were divided into three sections, and individual item scores were agreed upon among four pharmacists with experience in the conduct of clinical trials at two different centres. A final version of the tool, named Pharm-CAT, was approved. The pharmacists were instructed to use Pharm-CAT to assign a score to each new sponsored trial. To determine the cut-offs for the complexity categories, the scores were sorted in ascending order and the cut-offs corresponding to the first and third tertiles of the score distribution were selected. To verify the reproducibility of the results, Pharm-CAT was applied by two pharmacists independently for each trial. Pharm-CAT proved to be user-friendly. Sixty clinical trials were evaluated and a total of 120 scores were recorded. Low-complexity scores ranged from 0 to 19, medium-complexity scores ranged from 20 to 25, and high-complexity scores were 26 or higher. The average score recorded was 22.88 points. Prospective multicentre validation of Pharm-CAT is needed to confirm its applicability.
Good Handling Practices Have Positive Impacts on Dairy Calf Welfare
Authors: Silva-Antunes Lívia C. M. , Ceballos Maria Camila , Negrão João A. , Paranhos da Costa Mateus J. R.
Published: May 2024

The objective was to evaluate the effects of good handling practices on dairy calf welfare. Forty-eight crossbred dairy calves were assigned to two treatments: conventional handling (CH): calves kept in individual pens, fed milk replacer in buckets without nipples and abruptly weaned; or good handling practices (GHP): calves kept in group pens, fed milk replacer in buckets with nipples, given daily tactile stimulation during feeding, and progressive weaning. Calf welfare was assessed from birth to 120 days of age, based on: health (plasma concentrations of glucose and IgG, and occurrences of diarrhea, pneumonia, tick-borne disease, or death); physiology (heart rate [HR], respiratory rate [RR], and rectal temperature [RT]); behavior (flight distance [FD], latencies for first movement [LM] and to hold the calf in a pen corner [LH], and total time a calf allowed touching [TTT]); and performance indicators (body weight, average daily gain, and weaning success at 70 days of age). Calves in the GHP treatment had a lower HR at 30 days of age, shorter FD and LH, longer TTT, and lower RR and RT than CH (p < 0.05). However, health, deaths and performance indicators did not differ (p > 0.05) between treatments. Based on various indicators, GHP improved dairy calf welfare.
The Incidence of Thyroid Cancer in Bethesda III Thyroid Nodules: A Retrospective Analysis at a Single Endocrine Surgery Center
Authors: Hassan Iyad , Hassan Lina , Balalaa Nahed , Askar Mohamad , Alshehhi Hussa , Almarzooqi Mohamad
Published: May 2024

Background: Fine-needle aspiration cytology (FNAC) is widely used to diagnose and monitor thyroid nodules. The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) is the standard for interpreting FNAC specimens. The risk of malignancy in Bethesda III nodules, also known as Atypia of Undetermined Significance (AUS), varies significantly throughout several studies published worldwide. This retrospective study examines the risk of cancer in thyroid FNAC categorized as Bethesda III as identified in the final histopathology of thyroidectomy specimens at a single endocrine surgery center. Methods: This retrospective cohort analysis included 1038 consecutive patients who underwent elective thyroid surgery with complete follow-up data between January 2020 and March 2024. Preoperative data on clinical and pathological characteristics have been collected. The final histopathology report from the thyroidectomy specimen was compared to the results of the preoperative FNAC on nodules that were judged to be Bethesda category III. Statistical methods were performed using SPSS version 29. Results: A total of 670 ultrasound-guided FNACs (64.5%) performed during the study period were included in the final analysis. The study population was predominantly female, represented by 79.6% of patients with a mean age of 42.5 (SD 12.1), while 20.4% were male and significantly older with mean age of 45.13 years (p = 0.02). The FNAC inadequacy rate was 5.1%, which was associated with a high risk of malignancy (6 out of 34; 17.6%). Out of the total sample size of 170 patients classified as group III, 57 were found to have malignancies in final surgical histopathology, representing 33.5% of the cases within this category. The secondary gender-related outcome analysis showed that female patients classified under the Bethesda II category had a significantly higher risk of malignancy, with a rate of 21.2%, compared to males who had a malignancy rate of 3.4% in the same Bethesda category (p = 0.001, chi-square test). However, the female patients exhibited prognostically superior non-invasive tumors compared to male individuals (p = 0.02, chi-square test). Conclusion: This study’s results indicate that Bethesda categories II and III are associated with a higher risk of malignancy in comparison to the reports of the first and third editions of the TBSRTC, particularly for female patients classified under category II.
Development of an Antigen Capture Lateral Flow Immunoassay for the Detection of Burkholderia pseudomallei
Authors: Nualnoi Teerapat , Wongwitwichot Paweena , Kaewmanee Siriluk , Chanchay Pornchanan , Wongpanti Nattapong , Ueangsuwan Tossapol , Siangsanor Rattikarn , Chotirouangnapa Wannittaya , Saechin Tanatchaporn , Thungtin Suwanna , Szekely Jidapa , Wattanachant Chaiyawan , Saechan Vannarat
Published: May 2024

Early diagnosis is essential for the successful management of Burkholderia pseudomallei infection, but it cannot be achieved by the current gold standard culture technique. Therefore, this study aimed to develop a lateral flow immunoassay (LFIA) targeting B. pseudomallei capsular polysaccharide. The development was performed by varying nitrocellulose membrane reaction pads and chase buffers. The prototype LFIA is composed of Unisart CN95 and chase buffer containing tris-base, casein, and Surfactant 10G. The assay showed no cross-reactivity with E. coli, S. aureus, P. aeruginosa, and P. acne. The limit of detections (LODs) of the prototype LFIA was 107 and 106 CFU/mL B. pseudomallei in hemoculture medium and artificial urine, respectively. These LODs suggest that this prototype can detect melioidosis from positive hemoculture bottles but not straight from urine. Additionally, these LODs are still inferior compared to Active Melioidosis Detect (AMDTM). Overall, this prototype holds the potential to be used clinically with hemoculture bottles. However, further improvements should be considered, especially for use with urine samples.
A Method for Solving Problems in Acquiring Communication Logs on End Hosts
Authors: Fukuta Youji , Shiraishi Yoshiaki , Hirotomo Masanori , Mohri Masami
Published: May 2024

In the process of collecting evidence of activities and events in network devices, there are problems with content and storage, and we aim to solve the problems faced by network devices in network forensics. In this paper, we propose a simple method for solving the problems with content and storage in acquiring communication logs on end hosts, implement a sniffing tool that captures raw packets with communication event control, compare it with existing tools, and conduct experiments and considerations. Through these experiments and considerations, we confirmed that the proposed communication log acquisition method can be implemented on the end host, and that the problem can be solved by using a tool that implements the proposed method. Also, we confirmed that it can be applied to real-world communication log collection scenarios, and that it can coexist with existing systems and tools that collect communication logs.
Evaluating an Adapted Physical Activity Program for University Students and Staff Living with a Physical Disability and/or Chronic Condition through a Self-Determination Theory Lens
Authors: Liska Tayah M. , Pastore Olivia L. , Bedard Gabrielle D. , Ceh Crystal , Freilich Leah , Desjourdy Rachel , Sweet Shane N.
Published: May 2024

The purpose of this mixed-method study was to (1) examine the effect of an adapted physical activity program, Fitness Access McGill (FAM), on leisure-time physical activity (LTPA), autonomous and controlled motivation, and the basic psychological needs of self-determination theory among university students/staff with a physical disability and/or chronic conditions, and (2) explore participants’ experiences after completing FAM. Nineteen participants completed validated questionnaires for all study outcomes pre- and post-FAM. Nine participants partook in a 30–60 min semi-structured interview conducted within three months of completing FAM. Quantitative data were analyzed using repeated measures effect size calculations. Qualitative data were analyzed using directed content analysis. Participants reported an increase in total LTPA (dRMpooled = 0.58), with the greatest positive change on strenuous intensity (dRMpooled = 0.81). Large effects were found for changes in autonomous motivation (dRMpooled = 0.52), autonomy (dRMpooled = 0.79), competence (dRMpooled = 0.79), and relatedness (dRMpooled = 0.89). Participants reported FAM being supportive towards their psychological needs, the development of a LTPA routine, and enhanced overall well-being. Future research can be built upon this study to develop a robust understanding as to how need-supportive, adapted LTPA programs could be implemented within community settings or out-patient rehabilitation to support exercise engagement, physical health and overall well-being among adults with disabilities.
The Use of Anabolic Steroids by Bodybuilders in the State of Sergipe, Brazil
Authors: dos Santos Josué Cruz , Souza Erivaldo de , Meneses-Santos Daniela , Carvalho Carla Roberta de Oliveira , dos Santos Jymmys Lopes , Aidar Felipe J. , Marçal Anderson Carlos
Published: May 2024

Bodybuilding, as a high-performance sport, requires regular strength and resistance exercises with the principal objective of increasing muscle hypertrophy. However, many bodybuilders resort to the use of anabolic-androgenic steroids (AASs) to improve their performance in a short period of time. This study employs a survey-type, cross-sectional, descriptive–analytical method to evaluate the profile of bodybuilding athletes in the State of Sergipe, Brazil, and verify the level of knowledge/awareness about the health risks and impacts resulting from the use of such substances. Finite- and convenience-type populations are assessed, including individuals of both sexes, aged older than 18 years, self-declared bodybuilding athletes residing in the State of Sergipe, Brazil, and participating in regional and/or state competitions. As a result, no significant relationships were determined between sex (p = 0.492), age (p = 0.460), family income (p = 0.141), and medical follow-up sessions. For the variables level of education and medical follow-up vs. no follow-up sessions, a significant result was achieved (p = 0.01), with 74.3% of individuals reporting having follow-up treatment and 25.7% responding that they had no follow-up treatment, a percentage representing the group that completed their higher education. The substances most used by the athletes were Sustanon 250 or Durateston, Nandrolone Decanoate (Deca or Deca-Durabolin), and Testosterone. The most-reported acute side effects were acne at 33.8% (n = 20), irritability at 32.1% (n = 19), alopecia (hair loss), and nervousness at 23.7% (n = 14). The most-reported chronic side effects were arterial hypertension at 36.0% (n = 9), liver disease at 28.0% (n = 7), and cancer (non-specific) at 8.0% (n = 2). We concluded that, regardless of the athletes’ socioeconomic profiles, the use of AASs was high, with two or more substances being used in combination and for a prolonged period. Thus, it is necessary to promote awareness campaigns regarding the use of AASs and their effects on high-performance and recreational athletes.
Effect of Biochar on Anaerobic Co-Digestion of Untreated Sewage Sludge with Municipal Organic Waste under Mesophilic Conditions
Authors: Ramírez Joisleen , Deago Euclides , James Rivas Arthur Mc Carty
Published: May 2024

Anaerobic digestion (AD) is a biological process that occurs in the limited presence of oxygen. This process involves various difficulties during the operation, such as acidification and increased concentration of volatile fatty acids, which can inhibit methane production. Therefore, in this work, the impact of biochar on the co-digestion of untreated sludge and residual biomass under mesophilic conditions was studied. For the production of biochar, the gasification process was used at different temperatures: 759 °C (BL), 798 °C (BM), and 888 °C (BH). This biochar was added in concentrations of 0 g/L, 3.33 g/L, and 6.67 g/L at the beginning of the co-digestion process. The results showed that a concentration of 6.67 g/L with BH biochar increased the PBM by 18% compared to the control sample and reduced the chemical oxygen demand (COD) by 88%. In addition, there was a reduction of volatile fatty acids (VFA) of 42.75%. Furthermore, FTIR analysis demonstrated that biochar has appropriate functional groups for this process. These data suggest a good interaction of biochar with the mixture of sludge and municipal waste, indicating that biochar can improve the anaerobic co-digestion of untreated sludge and municipal waste.
Analysis of the Flow Behavior and Pressure Fluctuation of a Pump Turbine with Splitter Blades in Part-Load Pump Mode
Authors: Xiao Wei , Ren Shaocheng , Chen Liu , Yan Bin , Zhu Yilin , Xiao Yexiang
Published: May 2024

The internal flow of a pump turbine is unstable in part-load pump mode for small guide-vane openings, and the strong vibration caused by pressure pulsation is related to the safe and stable operation of the unit. A pump turbine with a six-splitter-blade runner was chosen for unsteady simulation analyses. A standard k-epsilon turbulence model was adopted to study the unsteady flow and pressure pulsation in part-load pump mode. The predicted results show that the flow in the draft tube and the runner with splitter blades was relatively stable and the flow of the blade-to-blade channel was symmetrical. When the inlet and outlet velocity distribution of the vanes was not uniform, a vortex began to form in the stay-vane domain. The reason for this vortex formation is explained, and it is pointed out that the existence of the vortex and backflow leads to uneven velocity distribution. The unsteady calculation results showed that the pressure-pulsation peak-to-peak amplitudes in the vaneless area and guide vanes were much higher than those of other monitor points because of rotor–stator interference between the rotating runner and the vanes. In addition, the pulsation characteristics of the monitor points at different circumferential positions in the vaneless region were quite different. In the vaneless area, the velocity gradient along the circumferential direction was very large, and there was a phenomenon of backflow. Also, the pressure pulsation was 0.2 times that of the runner rotational frequency, and the blade-passing frequency was a third-order frequency. At the outlet of the guide vane, the pressure pulsation was mainly of a low frequency with a complex vortex flow. Finally, the pressure pulsation began to decrease rapidly in the stay-vane region.
Spray Characterization of Direct Hydrogen Injection as a Green Fuel with Lower Emissions
Authors: Payri Raul , Novella Ricardo , Nasser Khodor I. , Bori-Fabra Oscar
Published: May 2024

A viable green energy source for heavy industries and transportation is hydrogen. The internal combustion engine (ICE), when powered by hydrogen, offers an economical and adaptable way to quickly decarbonize the transportation industry. In general, two techniques are used to inject hydrogen into the ICE combustion chamber: port injection and direct injection. The present work examined direct injection technology, highlighting the need to understand and manage hydrogen mixing within an ICE’s combustion chamber. Before combusting hydrogen, it is critical to study its propagation and mixture behavior just immediately before burning. For this purpose, the DI-CHG.2 direct injector model by BorgWarner was used. This injector operated at 35 barG and 20 barG as maximum and minimum upstream pressures, respectively; a 5.8 g/s flow rate; and a maximum tip nozzle temperature of 250 °C. Experiments were performed using a high-pressure and high-temperature visualization vessel available at our facility. The combustion mixture prior to burning (spray) was visually controlled by the single-pass high-speed Schlieren technique. Images were used to study the spray penetration (S) and spray volume (V). Several parameters were considered to perform the experiments, such as the injection pressure (Pinj), chamber temperature (Tch), and the injection energizing time (Tinj). With pressure ratio and injection time being the parameters commonly used in jet characterization, the addition of temperature formed a more comprehensive group of parameters that should generally aid in the characterization of this type of gas jets as well as the understanding of the combined effect of the rate of injection on the overall outcome. It was observed that the increase in injection pressure (Pinj) increased the spray penetration depth and its calculated volume, as well as the amount of mass injected inside the chamber according to the ROI results; furthermore, it was also observed that with a pressure difference of 20 bar (the minimum required for the proper functioning of the injector used), cyclic variability increased. The variation in temperature inside the chamber had less of an impact on the spray shape and its penetration; instead, it determined the velocity at which the spray reached its maximum length. In addition, the injection energizing time had no effect on the spray penetration.
The Ecumene: A Research Program for Future Knowledge and Governance
Authors: Seixas Paulo Castro , Lobner Nadine
Published: May 2024

The ecumene defines a beyond-border space of strong cultural encounters, flows, and merging, grounded within the traditions of world-systems, globalization, transnationalism, and cosmopolitanism discourses. Furthermore, the ecumene links directly with international regions as core political platforms in the making. As such, there are several ecumenes on the forefront, as evidenced by the literature, which can be clustered into ideal types. Epistemologically, it is a relevant concept and tool for a science-of-the-future that focuses on conviviality and transformation for the yet-to-come. Analytically, the ecumene has a descriptive, normative, and critical dimension, and can be empirically accessed through operational concepts such as triggers, hubs, and types of beyond-border conviviality. To apply the ecumene as a research program means to detect convivial common-sense spaces within the global context.
Displacement Micropump with Check Valves for Diabetes Care—The Challenge of Pumping Insulin at Negative Pressure
Authors: Chappel Eric
Published: May 2024

The displacement micropump with passive check valves is an attractive solution for precise insulin infusion in patients with type I diabetes. Unlike most insulin pumps that push insulin from a cartridge using a piston, a displacement micropump will first pull insulin from the reservoir before infusing it into the patient. This dual sequence introduces new challenges in terms of insulin stability, notably if the reservoir is not pressurized. After an introduction to displacement micropumps and a brief review of the insulin degradation mechanism, micropump design rules are discussed in light of microfluidic theory.
Cushion Gas Consideration for Underground Hydrogen Storage
Authors: Prigmore Sadie , Okon-Akan Omolabake Abiodun , Egharevba Imuentinyan P. , Ogbaga Chukwuma C. , Okoye Patrick U. , Epelle Emmanuel , Okolie Jude A.
Published: May 2024

Due to the increasing world population and environmental considerations, there has been a tremendous interest in alternative energy sources. Hydrogen plays a major role as an energy carrier due to its environmentally benign nature. The combustion of hydrogen releases water vapor while it also has a vast industrial application in aerospace, pharmaceutical, and metallurgical industries. Although promising, hydrogen faces storage challenges. Underground hydrogen storage (UHS) presents a promising method of safely storing hydrogen. The selection of the appropriate cushion gas for UHS is a critical aspect of ensuring the safety, efficiency, and reliability of the storage system. Cushion gas plays a pivotal role in maintaining the necessary pressure within the storage reservoir, thereby enabling consistent injection and withdrawal rates of hydrogen. One of the key functions of the cushion gas is to act as a buffer, ensuring that the storage pressure remains within the desired range despite fluctuations in hydrogen demand or supply. This is achieved by alternately expanding and compressing the cushion gas during the injection and withdrawal cycles, thereby effectively regulating the overall pressure dynamics within the storage facility. Furthermore, the choice of cushion gas can have significant implications on the performance and long-term stability of the UHS system. Factors such as compatibility with hydrogen, cost-effectiveness, availability, and environmental impact must be carefully considered when selecting the most suitable cushion gas. The present study provides a comprehensive review of different types of cushion gases commonly used in UHS, including nitrogen, methane, and carbon dioxide. By examining the advantages, limitations, and practical considerations associated with each option, the study aims to offer valuable insights into optimizing the performance and reliability of UHS systems. Ultimately, the successful implementation of UHS hinges not only on technological innovation but also on strategic decisions regarding cushion gas selection and management. By addressing these challenges proactively, stakeholders can unlock the full potential of hydrogen as a clean and sustainable energy carrier, thereby contributing to the global transition towards a low-carbon future.
Influence of a Shaft Shoulder on the Torsional Load-Bearing Behaviour of Trochoidal Profile Contours as Positive Shaft–Hub Connections
Authors: Ziaei Masoud , Selzer Marcus , Sommer Heiko
Published: May 2024

Shafts with a stepped shoulder are particularly well known in the field of drive technology. In combination with a form-fit shaft–hub connection, the shaft shoulder fixes the hub on the shaft as well as being responsible for the absorption of the axial forces. With profiled shafts, there is a notch overlay in the shaft shoulder, involving the shaft shoulder and profile. If the hub is also connected with the profiled shaft, the hub edge acts as an additional notch in the shaft shoulder area. The multiple resulting notches have not previously been part of research activities in the field of innovative trochoidal profile connections. Compared to conventional positive-locking connections, such as the keyway connection or the involute splined shaft profile, the favourable features of trochoidal profiles have only been based on connections with stepless shafts without a shoulder in previous studies. Accordingly, this article addresses numerical and experimental investigations of trochoidal profile connections with offset shafts for pure torsional loading. Focusing on a hybrid trochoid with four eccentricities and six drivers, a well-founded numerical and experimental investigation was carried out with numerous fatigue tests. In addition, the influence of a shaft shoulder was also demonstrated on simple epitrochoidal and hypotrochoidal profiles.
Continuous Leaf Area Index (LAI) Observation in Forests: Validation, Application, and Improvement of LAI-NOS
Authors: Gao Zhentao , Chen Yunping , Zhang Zhengjian , Duan Tianxin , Chen Juncheng , Li Ainong
Published: May 2024

The leaf area index (LAI) is one of the core parameters reflecting the growth status of vegetation. The continuous long-term observation of the LAI is key when assessing the dynamic changes in the energy exchange of ecosystems and the vegetation’s response indicators to climate change. The errors brought about by non-standard operations in manual LAI measurements hinder the further research utilization of this parameter. The long-term automatic LAI observation network is helpful in reducing errors from manual measurements. To further test the applicability of automatic LAI observation instruments in forest environments, this study carried out comparative validation research of the LAI-NOS (LAI automatic network observation system) at the Wanglang Mountain Ecological Remote Sensing Comprehensive Observation Station, China, comparing it with the results measured by the LAI-2200 Plant Canopy Analyzer (LI-COR, Lincoln, NE, USA), the LAI-probe handheld instrument, and a fisheye lens digital camera (DHP method). Instead of using the original “smoothest window” method, a new method, the “sunrise–sunset” method, is used to extract daily LAI-NOS LAI, and the corresponding confidence level is used to filter the data. The results of the data analysis indicate the following: LAI-NOS has a high data stability. The automatically acquired daily data between two consecutive days has a small deviation and significant correlations. Single-angle/multi-angle LAI measurement results of the LAI-NOS have good correlations with the LAI-2200 (R2 = 0.512/R2 = 0.652), the LAI-probe (R2 = 0.692/R2 = 0.619), and the DHP method (R2 = 0.501/R2 = 0.394). The daily LAI obtained from the improved method, when compared to the original method, both show the same vegetation growth trend. However, the improved method has a smaller dispersion. This study confirms the stability and accuracy of automatic observation instruments in mountainous forests, demonstrating the distinct advantages of automatic measurement instruments in the long-term ground observation of LAIs.
Detection of the Pine Wilt Disease Using a Joint Deep Object Detection Model Based on Drone Remote Sensing Data
Authors: Wu Youping , Yang Honglei , Mao Yunlei
Published: May 2024

Disease and detection is crucial for the protection of forest growth, reproduction, and biodiversity. Traditional detection methods face challenges such as limited coverage, excessive time and resource consumption, and poor accuracy, diminishing the effectiveness of forest disease prevention and control. By addressing these challenges, this study leverages drone remote sensing data combined with deep object detection models, specifically employing the YOLO-v3 algorithm based on loss function optimization, for the efficient and accurate detection of tree diseases and pests. Utilizing drone-mounted cameras, the study captures insect pest image information in pine forest areas, followed by segmentation, merging, and feature extraction processing. The computing system of airborne embedded devices is designed to ensure detection efficiency and accuracy. The improved YOLO-v3 algorithm combined with the CIoU loss function was used to detect forest pests and diseases. Compared to the traditional IoU loss function, CIoU takes into account the overlap area, the distance between the center of the predicted frame and the actual frame, and the consistency of the aspect ratio. The experimental results demonstrate the proposed model’s capability to process pest and disease images at a slightly faster speed, with an average processing time of less than 0.5 s per image, while achieving an accuracy surpassing 95%. The model’s effectiveness in identifying tree pests and diseases with high accuracy and comprehensiveness offers significant potential for developing forest inspection protection and prevention plans. However, limitations exist in the model’s performance in complex forest environments, necessitating further research to improve model universality and adaptability across diverse forest regions. Future directions include exploring advanced deep object detection models to minimize computing resource demands and enhance practical application support for forest protection and pest control.
A Variational Surface-Evolution Approach to Optimal Transport over Transitioning Compact Supports with Domain Constraints
Authors: Yezzi Anthony
Published: May 2024

We examine the optimal mass transport problem in Rn between densities with transitioning compact support by considering the geometry of a continuous interpolating support boundary Γ in space-time within which the mass density evolves according to the fluid dynamical framework of Benamou and Brenier. We treat the geometry of this space-time embedding in terms of points, vectors, and sets in Rn+1=R×Rn and blend the mass density and velocity as well into a space-time solenoidal vector field W|Ω→Rn+1 over a compact set Ω⊂Rn+1. We then formulate a joint optimization for W and its support using the shaped gradient of the space-time surface Γ outlining the support boundary ∂Ω. This easily accommodates spatiotemporal constraints, including obstacles or mandatory regions to visit.
Existence Results Related to a Singular Fractional Double-Phase Problem in the Whole Space
Authors: Alsaedi Ramzi
Published: May 2024

In this paper, we will study a singular problem involving the fractional (q1(x,.)-q2(x,.))-Laplacian operator in the whole space RN,(N≥2). More precisely, we combine the variational method with monotonicity arguments to prove that the associated functional energy admits a critical point, which is a weak solution for such a problem.
Dynamics for a Nonlinear Stochastic Cholera Epidemic Model under Lévy Noise
Authors: Ain Qura Tul , Din Anwarud , Qiang Xiaoli , Kou Zheng
Published: May 2024

In this study, we develop a comprehensive mathematical model to analyze the dynamics of epidemic cholera, characterized by acute diarrhea due to pathogen overabundance in the human body. The model is first developed from a deterministic point of view, and then it is modified to include the randomness by stochastic differential equations. The study selected Lévy noise above other well-known types of noise, emphasizing its importance in epidemic modeling. Besides presenting a biological justification for the stochastic system, we demonstrate that the equivalent deterministic model exhibits possible equilibria. The introduction is followed by theoretical analysis of the model. Through rigorous analysis, we establish that the stochastic model ensures a unique global solution. Lyapunov function theory is applied to construct necessary conditions, which on average, guarantee the model’s stability for R0s>1. Our findings suggest the likelihood of eradicating the disease when Rs is below one, a significant insight supported by graphical simulations of the model. Graphical illustrations were generated from simulating the model in order to increase the analytical results’ robustness. This work provides a strong theoretical framework for a thorough comprehension of a range of such diseases. This research not only provides a deeper understanding of cholera dynamics but also offers a robust theoretical framework applicable to a range of similar diseases, alongside a novel approach for constructing Lyapunov functions for nonlinear models with random disturbances.