bluuebunny/crossref_metadata_2025 huggingface dataset:
Photosynthetic Carbon Uptake Correlates with Cell Protein Content during Lipid Accumulation in the Microalga Chlorella vulgaris NIES 227
Authors: Chambonniere Paul , Ramírez-Romero Adriana , Dimitriades-Lemaire Alexandra , Sassi Jean-François , Delrue Florian
Published: November 2022

Large-scale microalgae cultivation for biofuel production is currently limited by the possibility of maintaining high microalgae yield and high lipid content, concomitantly. In this study, the physiological changes of Chlorella vulgaris NIES 227 during lipid accumulation under nutrient limitation was monitored in parallel with the photosynthetic capacity of the microalgae to fix carbon from the proxy of oxygen productivity. In the exponential growth phase, as the biomass composition did not vary significantly (approx. 53.6 ± 7.8% protein, 6.64 ± 3.73% total lipids, and 26.0 ± 9.2% total carbohydrates of the total biomass dry-weight), the growth capacity of the microalgae was preserved (with net O2 productivity remaining above (4.44 ± 0.93) × 10−7 g O2·µmol PAR−1). Under nutrient limitation, protein content decreased (minimum of approx. 18.6 ± 6.0%), and lipid content increased (lipid content up to 56.0 ± 0.8%). The physiological change of the microalgae was associated with a loss of photosynthetic activity, down to a minimum (1.27 ± 0.26) × 10−7 g O2·µmol PAR−1. The decrease in photosynthetic O2 productivity was evidenced to correlate to the cell internal-protein content (R2 = 0.632, p = 2.04 × 10−6, N = 25). This approach could serve to develop productivity models, with the aim of optimizing industrial processes.
Plant Antioxidants in Dry Fermented Meat Products with a Healthier Lipid Profile
Authors: Selani Miriam M. , Herrero Ana M. , Ruiz-Capillas Claudia
Published: November 2022

Consumers’ perception of meat products has changed in recent years, which has led to an increased interest in healthier meat products. In response to this demand, academia and industry have made efforts to reformulate meat products, especially dry fermented meat products, which are known for their high fat contents, mainly saturated fat. The use of plant or marine oils stabilized in emulsion gels (EGs) or oil-bulking agents (OBAs) as animal fat replacers has been one of the most advantageous strategies to reformulate dry fermented meat products with a healthier lipid content (quality and quantity), but an increase in their polyunsaturated fatty acid content can trigger a significant increase in lipid oxidation, negatively affecting sensory and nutritional quality. The use of antioxidants is the main strategy to delay this deteriorative reaction, but the controversy around the safety and toxicity of synthetic antioxidants has driven consumers and industry toward the use of plant antioxidants, such as phenolic compounds, carotenoids, and some vitamins and minerals. This review provides information about the use of plant antioxidants to control lipid oxidation of dry fermented meat products with healthier lipids.
Cobalt- and Copper-Based Chemiresistors for Low Concentration Methane Detection, a Comparison Study
Authors: Chesler Paul , Hornoiu Cristian , Anastasescu Mihai , Calderon-Moreno Jose Maria , Gheorghe Marin , Gartner Mariuca
Published: November 2022

Methane is a colorless/odorless major greenhouse effect gas, which can explode when it accumulates at concentrations above 50,000 ppm. Its detection cannot be performed without specialized equipment, namely sensing devices. A series of MOX sensors (chemiresistors type), with CoO and CuO sensitive films were obtained using an eco-friendly and low-cost deposition technique (sol–gel). The sensing films were characterized using AFM and SEM as thin film. The transducers are based on an alumina wafer, with Au or Pt interdigital electrodes (IDE) printed onto the alumina surface. The sensor response was recorded upon sensor exposure to different methane concentrations (target gas) under lab conditions (dried target and carrier gas from gas cylinders), in a constant gas flow, with target gas concentrations in the 5–2000 ppm domain and a direct current (DC) applied to the IDE as sensor operating voltage. Humidity and cross-sensitivity (CO2) measurements were performed, along with sensor stability measurements, to better characterize the obtained sensors. The obtained results emphasize good 3-S sensor parameters (sensitivity, partial selectivity and stability) and also short response time and complete sensor recovery, completed by a low working temperature (220 °C), which are key factors for further development of a new commercial chemiresistor for methane detection.
Thermosensitive In Situ Gels for Joint Disorders: Pharmaceutical Considerations in Intra-Articular Delivery
Authors: Koland Marina , Narayanan Vadakkepushpakath Anoop , John Anish , Tharamelveliyil Rajendran Arunraj , Raghunath Indu
Published: November 2022

The intra-articular administration of conventional drug solutions or dispersions in joint diseases such as osteoarthritis has a relatively short retention time and, therefore, limited therapeutic effect. Thermosensitive polymer solutions that exhibit a sol–gel phase transition near body temperature after injection can prolong drug retention by providing a depot from which the drug release is sustained while relieving inflammation and preventing degradation of the joint complex. Thermosensitive hydrogels have in recent times garnered considerable attention in the intra-articular therapeutics of joint diseases such as osteoarthritis. Among the stimuli-responsive gelling systems, most research has focused on thermosensitive hydrogels. These gels are preferred over other stimuli-sensitive hydrogels since they have well-controlled in situ gelling properties and are also easier to load with drugs. Temperature-sensitive polymers, such as block copolymers or poloxamers, are frequently used to modify their gelation properties, usually in combination with other polymers. They are compatible with most drugs but may pose formulation challenges in terms of their low-response time, highly fragile nature, and low biocompatibility. The stability and biodegradability of implant hydrogels can control the drug release rate and treatment efficacy. This review stresses the application of thermosensitive gels in joint disorders and summarizes recent developments for intra-articular application, including the incorporation of nanoparticles. The hydrogel composition, drug release mechanisms, and the challenges involved in their formulation and storage are also discussed.
The Study of the Influence of ZrO2 Precursor Type and the Temperature of Annealing on the Crystallization of the Tetragonal Polymorph of ZrO2 in Zirconia-Silica Gels
Authors: Adamczyk Anna
Published: November 2022

Materials of the ZrO2-SiO2 system were obtained by the sol-gel method applying two different types of ZrO2 precursors: zirconium (IV) n-propoxide Zr(OC3H7)4 and zirconium (IV) acetate Zr(OOC2H3)4 (organic acetic acid salt) while commonly used tetraethoxysilane TEOS was selected as SiO2 introducing one. ZrO2 concentration in synthesized samples varied from 20% to 50% (mol.). After drying for 28 days, all gels were annealed at 500 °C, 1000 °C, and 1200 °C in air. FTIR spectroscopy together with XRD diffraction was selected as the two main structure research methods. SEM microscopy was applied to analyze the local chemical compositions of samples and to observe the morphology of gels’ surfaces. The analysis of FTIR spectra and XRD diffraction patterns allowed us to recognize different ZrO2 polymorphs which appeared in the samples depending strongly as well on ZrO2 precursor type as on the temperature of annealing. Samples synthesized by using the zirconium (IV) n-propoxide contained both cubic and tetragonal zirconia phases in general but showed the tendency of the increasing t-ZrO2 content in gels richer in ZrO2 and heated up to 1200 °C. However, in materials obtained applying zirconium (IV) acetate, the first detected at 500 °C phase was t-ZrO2 which was then conversing to m-ZrO2 form with the increasing temperature in case of samples rich in ZrO2. Meanwhile, t-ZrO2 was the predominant phase in samples of the lower content of ZrO2 but annealed at higher temperatures. By the analysis of changes in band profiles and positions, one can draw conclusions that the structure of studied samples is mostly built up of an amorphous silica matrix, in which different types of zirconia polymorphs create their own crystal lattice. The presence of the particular polymorph depends strongly on the type of zirconia precursor and the temperature of annealing.
Task-Specific Phosphonium Iongels by Fast UV-Photopolymerization for Solid-State Sodium Metal Batteries
Authors: Porcarelli Luca , Olmedo-Martínez Jorge L. , Sutton Preston , Bocharova Vera , Fdz De Anastro Asier , Galceran Montserrat , Sokolov Alexei P. , Howlett Patrick C. , Forsyth Maria , Mecerreyes David
Published: November 2022

Sodium metal batteries are an emerging technology that shows promise in terms of materials availability with respect to lithium batteries. Solid electrolytes are needed to tackle the safety issues related to sodium metal. In this work, a simple method to prepare a mechanically robust and efficient soft solid electrolyte for sodium batteries is demonstrated. A task-specific iongel electrolyte was prepared by combining in a simple process the excellent performance of sodium metal electrodes of an ionic liquid electrolyte and the mechanical properties of polymers. The iongel was synthesized by fast (<1 min) UV photopolymerization of poly(ethylene glycol) diacrylate (PEGDA) in the presence of a saturated 42%mol solution of sodium bis(fluorosulfonyl)imide (NaFSI) in trimethyl iso-butyl phosphonium bis(fluorosulfonyl)imide (P111i4FSI). The resulting soft solid electrolytes showed high ionic conductivity at room temperature (≥10−3 S cm−1) and tunable storage modulus (104–107 Pa). Iongel with the best ionic conductivity and good mechanical properties (Iongel10) showed excellent battery performance: Na/iongel/NaFePO4 full cells delivered a high specific capacity of 140 mAh g−1 at 0.1 C and 120 mAh g−1 at 1 C with good capacity retention after 30 cycles.
DNA Gyrase Inhibitors Increase the Frequency of Bacteriophage-like RcGTA-Mediated Gene Transfer in Rhodobacter capsulatus
Authors: Bernelot-Moens Rachel , Beatty J. Thomas
Published: November 2022

Rhodobacter capsulatus produces a bacteriophage-like particle called the gene transfer agent (RcGTA) that mediates horizontal gene transfer. RcGTA particles transfer random ~4.5-kb fragments of genomic DNA that integrate into recipient genomes by allelic replacement. This work addresses the effect of sub-inhibitory concentrations of antibiotics on gene transfer by RcGTA. A transduction assay was developed to test the effects of various substances on gene transfer. Using this assay, low concentrations of DNA gyrase inhibitors were found to increase the frequency of gene transfer. Novobiocin was studied in more detail, and it was found that this antibiotic did not influence the production or release of RcGTA but instead appeared to act on the recipient cells. The target of novobiocin in other species has been shown to be the GyrB subunit of DNA gyrase (a heterotetramer of 2GyrA and 2GyrB). R. capsulatus encodes GyrA and GyrB homologues, and a GyrB overexpression plasmid was created and found to confer resistance to novobiocin. The presence of the overexpression plasmid in recipient cells greatly diminished the novobiocin-mediated increase in gene transfer, confirming that this effect is due to the binding of novobiocin by GyrB. The results of this work show that antibiotics affect gene transfer in R. capsulatus and may be relevant to microbial genetic exchange in natural ecosystems.
Pantoea agglomerans-Induced Dieback in Pistachio in Chile
Authors: Zamorano Alan , Zuñiga Tania , Córdova Pamela , Higuera Gastón , Bertaccini Assunta , Fiore Nicola
Published: November 2022

Pistachio crops have a great economic potential, as their global production has increased dramatically over the past few decades. Therefore, it is important to maintain the healthy phytosanitary status of pistachio crops. In a Chilean pistachio orchard, a dieback of the trees was observed, with blighting of twigs and severe necrosis in the trunk and twigs. Bacterial isolation, pathogenicity tests and molecular characterization were conducted to determine the causal agent of the observed disease. The bacterial isolation and analysis of 16S rRNA gene led to the identification of Pantoea genus bacteria. Pathogenicity tests carried out on fruits inoculated with Pantoea isolates induced large necrosis seven days post-inoculation. Further inoculations were carried out on pruning cuttings and on the trunk of 18-month-old pistachio plants. Thirty-one weeks after inoculation, necrotic lesions were observed in the wood of pistachio plants. Sequence analysis of housekeeping genes enabled the isolated bacterium to be identified as Pantoa agglomerans, and to verify its role as the causal agent of the pistachio dieback with necrotic lesions. This is the first report of an occurrence of P. agglomerans inducing dieback in pistachio.
Acute Effects of Augmented Reality Exergames versus Cycle Ergometer on Reaction Time, Visual Attention, and Verbal Fluency in Community Older Adults
Authors: Ferreira Soraia , Marmeleira José , del Pozo-Cruz Jesus , Bernardino Alexandre , Leite Nilton , Brandão Mafalda , Raimundo Armando
Published: November 2022

Background: This study aims to investigate the acute effects of an augmented reality session and a cycle ergometer session compared to no exercise on the reaction times, cognitive flexibility, and verbal fluency of older adults. Methods: Each participant did a familiarization with cognitive tests and the following three sessions: cycle ergometer, no exercise (control group), and augmented reality exergame (Portable Exergame Platform for Elderly) sessions. The participants were randomized in a within-group design into one of six possible combinations. Each moment had a 30 min duration, and after the session, the participants performed a Trail Making Test, a verbal fluency test, and a Deary–Liewald reaction time task. The data were analyzed with a one-way ANOVA with a Bonferroni adjustment. Results: The analysis between the no exercise, cycle ergometer, and augmented reality sessions showed no significant differences in the cognitive measurements. Conclusions: One session of the cycle ergometer exercise or the augmented reality exergames does not acutely improve the reaction times, cognitive flexibility, or verbal fluency in the elderly.
Best Educational Techniques in High-Fidelity Simulation According to Nursing Students—Adaptation and Validation of the Educational Practices Questionnaire (EPQ)
Authors: Zalewska Katarzyna , Zarzycka Danuta
Published: November 2022

The purpose of this study was to evaluate the best educational techniques used during high-fidelity simulations in training nursing students and to introduce the Polish version of the Educational Practices Questionnaire (EPQ) scale after its cultural adaptation and determination of its psychometric properties. The research group was composed of 361 second- and third-year nursing students in the licentiate program. The Cronbach’s alpha reliability coefficients for the adapted tool were 0.90 for the EPQ-PO (presence of educational techniques) subscale and 0.93 for the EPQ-IO subscale (importance of educational techniques). Additionally, the model fit rates in the CFA and EFA (as indicators of theoretical validity) proved to be high enough for the tool to be successfully used in scientific research. Preliminary results are also presented; the mean value of the response for the entire EPQ scale for both the PO and IO sections was M = 4.3, SD ± 0.90. The students in the study rated the opportunity for collaboration with other students and for working jointly on a given clinical situation very highly at M = 4.5, SD ± 0.70. The analysis of the scores of the individual scales and subscales of the EPQ showed statistically significant differences in results obtained for such variables as gender, place of residence, and year of studies.
Mass Spectrometry-Based Proteomic and Metabolomic Profiling of Serum Samples for Discovery and Validation of Tuberculosis Diagnostic Biomarker Signature
Authors: Fernandes Ana Filipa , Gonçalves Luís Gafeira , Bento Maria , Anjo Sandra I. , Manadas Bruno , Barroso Clara , Villar Miguel , Macedo Rita , Simões Maria João , Coelho Ana Varela
Published: November 2022

Tuberculosis (TB) is a transmissible disease listed as one of the 10 leading causes of death worldwide (10 million infected in 2019). A swift and precise diagnosis is essential to forestall its transmission, for which the discovery of effective diagnostic biomarkers is crucial. In this study, we aimed to discover molecular biomarkers for the early diagnosis of tuberculosis. Two independent cohorts comprising 29 and 34 subjects were assayed by proteomics, and 49 were included for metabolomic analysis. All subjects were arranged into three experimental groups—healthy controls (controls), latent TB infection (LTBI), and TB patients. LC-MS/MS blood serum protein and metabolite levels were submitted to univariate, multivariate, and ROC analysis. From the 149 proteins quantified in the discovery set, 25 were found to be differentially abundant between controls and TB patients. The AUC, specificity, and sensitivity, determined by ROC statistical analysis of the model composed of four of these proteins considering both proteomic sets, were 0.96, 93%, and 91%, respectively. The five metabolites (9-methyluric acid, indole-3-lactic acid, trans-3-indoleacrylic acid, hexanoylglycine, and N-acetyl-L-leucine) that better discriminate the control and TB patient groups (VIP > 1.75) from a total of 92 metabolites quantified in both ionization modes were submitted to ROC analysis. An AUC = 1 was determined, with all samples being correctly assigned to the respective experimental group. An integrated ROC analysis enrolling one protein and four metabolites was also performed for the common control and TB patients in the proteomic and metabolomic groups. This combined signature correctly assigned the 12 controls and 12 patients used only for prediction (AUC = 1, specificity = 100%, and sensitivity = 100%). This multiomics approach revealed a biomarker signature for tuberculosis diagnosis that could be potentially used for developing a point-of-care diagnosis clinical test.
Immune Response Gaps Linked to SARS-CoV-2 Infection: Cellular Exhaustion, Senescence, or Both?
Authors: Barbosa Leonardo Vinicius , Prá Daniele Margarita Marani , Nagashima Seigo , Pereira Marcos Roberto Curcio , Stocco Rebecca Benicio , da Silva Francys de Luca Fernandes , Cruz Milena Rueda , Dallagassa Djessyka , Stupak Thiago João , da Rosa Götz George Willian Xavier , Nasimoto Georgia Garofani , Cracco Luiz Augusto Fanhani , Silva Isabela Busto , de Moura Karen Fernandes , Deus Marina de Castro , Martins Ana Paula Camargo , Spitzenbergen Beatriz Akemi Kondo Van , Amaral Andréa Novais Moreno , de Paula Caroline Busatta Vaz , Machado-Souza Cleber , de Noronha Lucia
Published: November 2022

The COVID-19 pandemic, promoted by the SARS-CoV-2 respiratory virus, has resulted in widespread global morbidity and mortality. The immune response against this pathogen has shown a thin line between protective effects and pathological reactions resulting from the massive release of cytokines and poor viral clearance. The latter is possibly caused by exhaustion, senescence, or both of TCD8+ cells and reduced activity of natural killer (NK) cells. The imbalance between innate and adaptive responses during the early stages of infection caused by SARS-CoV-2 contributes to the ineffective control of viral spread. The present study evaluated the tissue immunoexpression of the tissue biomarkers (Arginase-1, CCR4, CD3, CD4, CD8, CD20, CD57, CD68, CD138, IL-4, INF-α, INF-γ, iNOS, PD-1, Perforin and Sphingosine-1) to understand the cellular immune response triggered in patients who died of COVID-19. We evaluated twenty-four paraffin-embedded lung tissue samples from patients who died of COVID-19 (COVID-19 group) and compared them with ten lung tissue samples from patients who died of H1N1pdm09 (H1N1 group) with the immunohistochemical markers mentioned above. In addition, polymorphisms in the Perforin gene were genotyped through Real-Time PCR. Significantly increased tissue immunoexpression of Arginase, CD4, CD68, CD138, Perforin, Sphingosine-1, and IL-4 markers were observed in the COVID-19 group. A significantly lower immunoexpression of CD8 and CD57 was also found in this group. It is suggested that patients who died from COVID-19 had a poor cellular response concerning viral clearance and adaptive response going through tissue repair.
Review on Catalytic Oxidation of VOCs at Ambient Temperature
Authors: Zhao Rui , Wang Han , Zhao Dan , Liu Rui , Liu Shejiang , Fu Jianfeng , Zhang Yuxin , Ding Hui
Published: November 2022

As an important air pollutant, volatile organic compounds (VOCs) pose a serious threat to the ecological environment and human health. To achieve energy saving, carbon reduction, and safe and efficient degradation of VOCs, ambient temperature catalytic oxidation has become a hot topic for researchers. Firstly, this review systematically summarizes recent progress on the catalytic oxidation of VOCs with different types. Secondly, based on nanoparticle catalysts, cluster catalysts, and single-atom catalysts, we discuss the influence of structural regulation, such as adjustment of size and configuration, metal doping, defect engineering, and acid/base modification, on the structure–activity relationship in the process of catalytic oxidation at ambient temperature. Then, the effects of process conditions, such as initial concentration, space velocity, oxidation atmosphere, and humidity adjustment on catalytic activity, are summarized. It is further found that nanoparticle catalysts are most commonly used in ambient temperature catalytic oxidation. Additionally, ambient temperature catalytic oxidation is mainly applied in the removal of easily degradable pollutants, and focuses on ambient temperature catalytic ozonation. The activity, selectivity, and stability of catalysts need to be improved. Finally, according to the existing problems and limitations in the application of ambient temperature catalytic oxidation technology, new prospects and challenges are proposed.
Development and Comprehensive Characteristics of Thermosensitive Liquid Suppositories of Metoprolol Based on Poly(lactide-co-glycolide) Nanoparticles
Authors: Bialik Maria , Proc Joanna , Zgadzaj Anna , Mulas Karolina , Kuras Marzena , Sobczak Marcin , Oledzka Ewa
Published: November 2022

Thermosensitive liquid suppositories (LSs) carrying the model antihypertensive drug metoprolol tartrate (MT) were developed and evaluated. The fundamental purpose of this work was to produce, for the first time, liquid MT suppositories based on biodegradable nanoparticles and optimize their rheological and mechanical properties for prospective rectal administration. The nanoparticle system was based on a biodegradable copolymer synthesized by ring opening polymerization (ROP) of glycolide (GL) and L,L-lactide (LLA). Biodegradable nanoparticles loaded with the model drug were produced by the o/o method at the first stage of the investigation. Depending on the concentration of the drug in the sample, from 66 to 91% of MT was released over 12 h, according to first-order kinetics. Then, thermosensitive LSs with MT-loaded biodegradable nanoparticles were obtained by a cold method and their mechanical and rheological properties were evaluated. To adjust the thermogelling and mucoadhesive properties for rectal administration, the amounts of major formulation components such as poloxamers (P407, P188), Tween 80, hydroxypropylcellulose (HPC), polyvinylpyrrolidone (PVP), and sodium alginate were optimized. The in vitro release results revealed that more than 80% of the MT was released after 12 h, following also first-order kinetics. It was discovered that the diffusion process was dominant. The drug release profile was mainly governed by the rheological and mechanical properties of the developed formulation. Such a novel, thermosensitive formulation might be an effective alternative to hypertension treatment, particularly for unconscious patients, patients with mental illnesses, geriatric patients, and children.
The Reentry Helix Is Potentially Involved in Cholesterol Sensing of the ABCG1 Transporter Protein
Authors: Hegyi Zoltán , Hegedűs Tamás , Homolya László
Published: November 2022

ABCG1 has been proposed to play a role in HDL-dependent cellular sterol regulation; however, details of the interaction between the transporter and its potential sterol substrates have not been revealed. In the present work, we explored the effect of numerous sterol compounds on the two isoforms of ABCG1 and ABCG4 and made efforts to identify the molecular motifs in ABCG1 that are involved in the interaction with cholesterol. The functional readouts used include ABCG1-mediated ATPase activity and ABCG1-induced apoptosis. We found that both ABCG1 isoforms and ABCG4 interact with several sterol compounds; however, they have selective sensitivities to sterols. Mutational analysis of potential cholesterol-interacting motifs in ABCG1 revealed altered ABCG1 functions when F571, L626, or Y586 were mutated. L430A and Y660A substitutions had no functional consequence, whereas Y655A completely abolished the ABCG1-mediated functions. Detailed structural analysis of ABCG1 demonstrated that the mutations modulating ABCG1 functions are positioned either in the so-called reentry helix (G-loop/TM5b,c) (Y586) or in its close proximity (F571 and L626). Cholesterol molecules resolved in the structure of ABCG1 are also located close to Y586. Based on the experimental observations and structural considerations, we propose an essential role for the reentry helix in cholesterol sensing in ABCG1.
Molecular Tuning of IR-786 for Improved Brown Adipose Tissue Imaging
Authors: Jo Gayoung , Kim Eun Jeong , Song Juhyun , Hyun Hoon
Published: November 2022

To overcome the limitations of brown adipose tissue (BAT) imaging with MRI and PET/CT, near-infrared (NIR) fluorescence imaging has been utilized in living animals because it is highly sensitive, noninvasive, nonradioactive, and cost-effective. To date, only a few NIR fluorescent dyes for detecting BAT have been reported based on the structure-inherent targeting strategy. Among them, IR-786, a commercial cyanine dye, was used firstly for quantitative NIR imaging of BAT perfusion in 2003. Owing to the high cytotoxicity, poor water solubility, and strong nonspecific background uptake of IR-786, the chemical structure of IR-786 should be redesigned to be more hydrophilic and less toxic so that it can show more BAT-specific accumulation. Here, we developed a BAT-specific NIR dye, BF800-AM, by incorporating the tyramine linker in the original structure of IR-786. After modifying the physicochemical properties of IR-786, in vivo results showed significant uptake of the newly designed BF800-AM in the BAT with improved signal-to-background ratio. Additional in vivo studies using mouse tumor models revealed that BF800-AM targeting to BAT is independent of tumor tissues, as distinct from IR-786 showing uptake in both tissues. Therefore, BF800-AM can be used for improved noninvasive visualization of BAT mass and activity in living animals.
Knockdown of Tet2 Inhibits the Myogenic Differentiation of Chicken Myoblasts Induced by Ascorbic Acid
Authors: Lu Yinglin , Shi Kai , Wang Haobin , Cao Heng , Li Fan , Zhou Jing , Yu Minli , Yu Debing
Published: November 2022

Ascorbic acid (also called Vitamin C, VC) strengthens the function of Tets families and directly increases DNA demethylation level to affect myogenic differentiation. However, the precise regulatory mechanism of DNA methylation in chicken myogenesis remains unclear. Results of present study showed that the mRNA expression of MyoD significantly decreased and MyoG and MyHC increased in myoblasts treated with 5 μM 5-azacytidine (5-AZA) and 5 μM VC (p < 0.05). Results also indicated the formation of myotubes was induced by 5-AZA or VC, but this effect was attenuated after knockdown of Tet2. In addition, the protein expression of TET2, DESMIN and MyHC was remarkable increased by the addition of 5-AZA or VC, and the upregulation was inhibited after knockdown of Tet2 (p < 0.05). DNA dot blot and immunofluorescence staining results suggested that the level of 5hmC was significantly increased when treated with 5-AZA or VC, even by Tet2 knockdown (p < 0.05). Moreover, 5-AZA and VC reduced the level of dimethylation of lysine 9 (H3K9me2) and trimethylation of lysine 27 of histone 3 (H3K27me3), and this inhibitory effect was eliminated after Tet2 knockdown (p < 0.05). These data indicated that Tet2 knockdown antagonized the increased levels of 5hmC and H3K27me3 induced by 5-AZA and VC, and eventually reduced myotube formation by modulating the expression of genes involved in myogenic differentiation. This study provides insights that epigenetic regulators play essential roles in mediating the myogenic program of chicken myoblasts.
iPLA2β-Null Mice Show HCC Protection by an Induction of Cell-Cycle Arrest after Diethylnitrosamine Treatment
Authors: Andrade Adriana , Poth Tanja , Brobeil Alexander , Merle Uta , Chamulitrat Walee
Published: November 2022

Group VIA phospholipase A2 (iPLA2β) play diverse biological functions in epithelial cells and macrophages. Global deletion in iPLA2β-null (KO) mice leads to protection against hepatic steatosis in non-alcoholic fatty liver disease, in part, due to the replenishment of the loss of hepatocellular phospholipids. As the loss of phospholipids also occurs in hepatocellular carcinoma (HCC), we hypothesized that global deletion in KO mice may lead to protection against HCC. Here, HCC induced by diethylnitrosamine (DEN) was chosen because DEN causes direct injury to the hepatocytes. Male wild-type (WT) and KO mice at 3–5 weeks of age (12–13 mice/group) were subjected to a single intraperitoneal treatment with 10 mg/kg DEN, and mice were killed 12 months later. Analyses of histology, plasma cytokines, and gene expression were performed. Due to the low-dose DEN used, we observed a liver nodule in 3 of 13 WT and 2 of 12 KO mice. Only one DEN-treated WT mouse was confirmed to have HCC. DEN-treated KO mice did not show any HCC but showed suppressed hepatic expression of cell-cycle cyclinD2 and BCL2 as well as inflammatory markers IL-1β, IL-10, and VCAM-1. Notably, DEN-treated KO mice showed increased hepatic necrosis and elevated levels of plasma lactate dehydrogenase suggesting an exacerbation of liver injury. Thus, global iPLA2β deficiency in DEN-treated mice rendered HCC protection by an induction of cell-cycle arrest. Our results suggest the role of iPLA2β inhibition in HCC treatment.
Evaluation of the Allelopathic Activity of Albizia procera (Roxb.) Benth. as a Potential Source of Bioherbicide to Control Weeds
Authors: Hossen Kawsar , Kato-Noguchi Hisashi
Published: November 2022

Agricultural production depends heavily on the application of synthetic herbicides. Using these herbicides results in the development of herbicide-resistant weeds, poses hazards to human and animal health, and pollutes the environment. To solve these problems, developing and using bioherbicides must be increased. Although different uses of Albizia procera have been well reported, its allelopathic activity against weeds and crop species has not. Hence, we evaluated the allelopathic activity of the A. procera plant and isolated its allelopathic compounds. Extracts of A. procera significantly suppressed the seedling growth of the tested species (cabbage, alfalfa, lettuce, barnyard grass, timothy, and Italian ryegrass). The seedling growth decreased with increasing extract concentrations. The concentrations required for 50% growth inhibition (I50 value) of the tested plants were 0.0225–0.4935 mg/mL. The A. procera extracts were separated using different column chromatography, and two active fractions (AP-5 and AP-7) were isolated. Cress seedling growth was completely restricted by fraction AP-5, and fraction AP-7 restricted the cress shoots to 83.10% and roots to 85.65% of the control treatment. The findings of this study indicate that A. procera extracts have allelopathic activity and these fractions might contribute to the activity.
Dose Optimization of Meropenem in Patients on Veno-Arterial Extracorporeal Membrane Oxygenation in Critically Ill Cardiac Patients: Pharmacokinetic/Pharmacodynamic Modeling
Authors: Kang Soyoung , Yang Seungwon , Hahn Jongsung , Jang June Young , Min Kyoung Lok , Wi Jin , Chang Min Jung
Published: November 2022

Background: Our objective was to determine an optimal dosage regimen of meropenem in patients receiving veno-arterial extracorporeal membrane oxygenation (V-A ECMO) by developing a pharmacokinetic/pharmacodynamic (PK/PD) model. Methods: This was a prospective cohort study. Blood samples were collected during ECMO (ECMO-ON) and after ECMO (ECMO-OFF). The population pharmacokinetic model was developed using nonlinear mixed-effects modeling. A Monte Carlo simulation was used (n = 10,000) to assess the probability of target attainment. Results: Thirteen adult patients on ECMO receiving meropenem were included. Meropenem pharmacokinetics was best fitted by a two-compartment model. The final pharmacokinetic model was: CL (L/h) = 3.79 × 0.44CRRT, central volume of distribution (L) = 2.4, peripheral volume of distribution (L) = 8.56, and intercompartmental clearance (L/h) = 21.3. According to the simulation results, if more aggressive treatment is needed (100% fT > MIC target), dose increment or extended infusion is recommended. Conclusions: We established a population pharmacokinetic model for meropenem in patients receiving V-A ECMO and revealed that it is not necessary to adjust the dosage depending on V-A ECMO. Instead, more aggressive treatment is needed than that of standard treatment, and higher dosage is required without continuous renal replacement therapy (CRRT). Also, extended infusion could lead to better target attainment, and we could provide updated nomograms of the meropenem dosage regimen.
Temporomandibular Disorders and Bruxism among Sex Workers—A Cross Sectional Study
Authors: Eli Ilana , Zigler-Garburg Adi , Winocur Efraim , Friedman-Rubin Pessia , Shalev-Antsel Tamar , Levartovsky Shifra , Emodi-Perlman Alona
Published: November 2022

Sex workers are a highly underprivileged population which is present all around the world. Sex work is associated with negative social stigma which affects all aspects of the sex workers’ lives including healthcare, service providers and police. The stigma may result in increased stress, mental health problems, feelings of isolation and social exclusion. In the present study, 36 sex workers (SW) and 304 subjects from the general population in Israel (GP) were evaluated for the presence of bruxism and Temporomandibular disorders (TMD), with the use of Diagnostic Criteria for Temporomandibular Disorders (DC/TMD- Axis I). When compared to the general population, sex workers presented larger maximal assisted mouth opening and higher prevalence of the following TMD diagnoses: Disc displacement with reduction, Myalgia, Myofascial pain with referral, Arthralgia (left and right) and Headache attributed to TMD. The odds of sex workers suffering from one of these diagnoses were twice to five times higher than those of the general population. The study shows that health problems of sex workers go beyond venereal diseases, HIV and mental disorders which are commonly studied. Oral health, TMD and oral parafunctions are some of the additional health issues that should be addressed and explored in this population.
Point-of-Care Virtual Surgical Planning and 3D Printing in Oral and Cranio-Maxillofacial Surgery: A Narrative Review
Authors: Ostaș Daniel , Almășan Oana , Ileșan Robert R. , Andrei Vlad , Thieringer Florian M. , Hedeșiu Mihaela , Rotar Horațiu
Published: November 2022

This paper provides an overview on the use of virtual surgical planning (VSP) and point-of-care 3D printing (POC 3DP) in oral and cranio-maxillofacial (CMF) surgery based on a literature review. The authors searched PubMed, Web of Science, and Embase to find papers published between January 2015 and February 2022 in English, which describe human applications of POC 3DP in CMF surgery, resulting in 63 articles being included. The main review findings were as follows: most used clinical applications were anatomical models and cutting guides; production took place in-house or as “in-house—outsourced” workflows; the surgeon alone was involved in POC 3DP in 36 papers; the use of free versus paid planning software was balanced (50.72% vs. 49.27%); average planning time was 4.44 h; overall operating time decreased and outcomes were favorable, though evidence-based studies were limited; and finally, the heterogenous cost reports made a comprehensive financial analysis difficult. Overall, the development of in-house 3D printed devices supports CMF surgery, and encouraging results indicate that the technology has matured considerably.
Buccolingual Inclination of Second Molars in Untreated Adolescents and Adults with Near Normal Occlusion: A CBCT Study
Authors: Li Chenshuang , Dimitrova Boryana , Boucher Normand S. , Chung Chun-Hsi
Published: November 2022

The mandibular second molars are lingually positioned relative to the alveolar ridge and have a limited amount of lingual alveolar bony support. As the maxillary second molars are articulated with the mandibular second molars, maintaining the normal buccolingual inclination of both maxillary and mandibular second molars would potentially help to not only optimize the masticatory function, but also avoid dehiscence and fenestration. The current study evaluated the buccolingual inclination of second molars in untreated adolescents and adults. One hundred and two Caucasian subjects with skeletal class I and minimum dental arch crowding/spacing were selected and divided into two groups: (1) adolescent group: age 12–18 years, N = 51 (21 females, 30 males); (2) adult group: age 19–65 years, N = 51 (40 females, 11 males). For each subject, the inclination for each second molar was measured as the angle between the long axis of each tooth and a vertical line on cone beam computed tomography images. The Mann–Whitney U test was used for intergroup comparisons. Maxillary second molars exhibited a buccal inclination of 15.30° in the adolescent group and 15.70° in the adult group. Mandibular second molars exhibited a lingual inclination of 17.05° in the adolescent group and 15.20° in the adult group. No statistically significant differences were detected between the age groups. In addition, a statistically significant difference was only found between genders in the adolescent group for the maxillary second molar inclination. In summary, maxillary second molars exhibited buccal inclination and mandibular second molars exhibited lingual inclination. The amount of buccolingual inclination of the second molars was similar in the adolescent and adult groups.
Prevalent Technique and Results of Hemorrhoidal Embolization
Authors: Buso Gil Silvia , Ferrer Puchol María , Solaz Solaz Jorge , Esteban Hernández Enrique
Published: November 2022

Hemorrhoids are blood cushions located in the anus and lower rectum, acknowledged as a common cause of bleeding, which can reduce quality of life. The development of minimally invasive techniques such as endovascular embolization of superior rectal artery, “Emborrhoid technique”, is an effective treatment, with no pain or ischemic complications, and allows quick patient recovery. Our purpose is to describe the general technique and discuss the results of the current literature.
RNA-Based Next-Generation Sequencing in the Somatic Molecular Testing of Non-Small-Cell Lung Cancer (NSCLC) in a Centralized Model: Real-World Data to Suggest It Is Time to Reconsider Testing Options
Authors: Finall Alison
Published: November 2022

Best practice in the management of non-squamous, non-small-cell lung cancer patients involves somatic testing for a range of molecular markers. Actionable oncogenic drivers of malignancy are increasingly being detected using RNA-based next-generation sequencing in the UK by referral to centralized genomic laboratory hubs. Recent audit data from the author’s case work have demonstrated an RNA sequencing failure rate of 35%. This article examines the real-world context, which may account for this failure rate, and discusses alternative options for patient care pathways.
Spinal Arachnoiditis in Patients with Coccidioidomycosis Meningitis—Analysis of Clinical and Imaging Features
Authors: Sivasubramanian Geetha , Kadakia Saurin , Kim Jani M. , Pervaiz Sarah , Yan Yueqi , Libke Robert
Published: November 2022

Background: Coccidioidomycosis meningitis (CM) is the most aggressive form of coccidioidomycosis, requiring lifelong antifungal treatment and often cerebrospinal fluid (CSF) diversion. Long-standing CM can be associated with spinal complications such as arachnoiditis. However, studies describing the frequency, clinical, and imaging characteristics of arachnoiditis in patients with CM are limited. Methods: We identified 133 patients with CM based on CSF culture, PCR, or serology between January 2010 and December 2020. Of these, 37 patients underwent spinal imaging. Data on demographics, risk factors, symptoms, antifungal therapy, surgical management, follow-up visits, adherence, serological trends, and imaging findings were reviewed. Results: Abnormal findings were observed in 30 of the 37 patients with CM who underwent spinal imaging. The imaging abnormalities noted in our study included leptomeningeal enhancement (53%), arachnoiditis (53%), syringomyelia (23%), cord signal abnormalities (10%), and osteomyelitis (7%). Of the 30 patients, 90% had symptoms, such as weakness, numbness, or urinary retention. The incidence of arachnoiditis in the present study was 12%. Higher initial CSF protein levels and intra cranial pressure were associated with a higher risk of developing arachnoiditis/syringomyelia. Management of CM was challenging, as evidenced by shunt failure (46%), medication non-compliance (57%), and lack of adequate follow-up (60%). Persistent disabilities were noted in 62% of the patients. Conclusions: Patients with CM develop spinal complications such as arachnoiditis, or syringomyelia. Many cases may go undetected due to lack of symptoms in early stages. CM management challenges such as shunt failure, lack of follow-up care, and medication noncompliance, were frequent.
Nontraumatic Spinal Cord Injury: Epidemiology, Etiology and Management
Authors: Molinares Diana M. , Gater David R. , Daniel Scott , Pontee Nicole L.
Published: November 2022

The spinal cord is a conduit within the central nervous system (CNS) that provides ongoing communication between the brain and the rest of the body, conveying complex sensory and motor information necessary for safety, movement, reflexes, and optimization of autonomic function. After a traumatic spinal cord injury (SCI), supraspinal influences on the peripheral nervous system and autonomic nervous system (ANS) are disrupted, leading to spastic paralysis, sympathetic blunting, and parasympathetic dominance, resulting in cardiac dysrhythmias, systemic hypotension, bronchoconstriction, copious respiratory secretions, and uncontrolled bowel, bladder, and sexual dysfunction. This article outlines the pathophysiology of the less reported nontraumatic SCI (NTSCI), its classification, its influence on sensory/motor function, and introduces the probable comorbidities associated with SCI that will be discussed in more detail in the accompanying manuscripts of this special issue. Finally, management strategies for NTSCI will be provided.
Unlocking Romania’s Forest-Based Bioeconomy Potential: Knowledge-Action-Gaps and the Way Forward
Authors: Giurca Alexandru , Nichiforel Liviu , Stăncioiu Petru Tudor , Drăgoi Marian , Dima Daniel-Paul
Published: November 2022

As national governments continue developing bioeconomy strategies, the forest-based sector becomes increasingly important. Romania’s forest sector can be at the heart of a sustainable circular-bioeconomy transition. However, despite recently launching its national forest strategy, the country has yet to produce a comprehensive bioeconomy strategy where the forest sector is properly acknowledged and integrated. Here, we discuss the potential opportunities and challenges for developing a national circular–bioeconomy strategy that builds around the forest-based sector. Methodologically, we build on qualitative insights from two foresight workshops conducted with 16 international experts. Conceptually, we draw on recent forest-based circular-bioeconomy literature, which we synthesize and use to complement the insights provided by workshop participants. Three main key findings emerged from this analysis: (i) Several knowledge gaps related to biomass availability, carbon storage, biodiversity status, ecosystem services, or governance arrangements persist. (ii) A circular forest bioeconomy must focus on regional and rural development, including both traditional wood use, as well as new wood-based products. Finally, (iii) the transition to a forest-based bioeconomy requires substantial investments in areas such as forest infrastructure, education, and labor force. Forward-looking policies can address these challenges by fostering new ways of thinking, collaborating and researching the bioeconomy. We anticipate our article to be a starting point for more informed discussions around the role of forests and the forest-based sector in Romania’s future bioeconomy strategy. Furthermore, as work around the implementation of the national forest strategy has recently commenced, the ideas discussed here could help decision-makers better integrate and coordinate national and European forest policies with bioeconomy ambitions.
Micro-Contact in Southern Italy: Language Change in Southern Lazio under Pressure from Italian
Authors: Colasanti Valentina
Published: November 2022

This paper explores a novel case of contact-induced change due to micro-contact within Italy, where various Italo-Romance languages coexist (Standard Italian, Italiano Regionale ‘regional Italian’, and numerous local languages). Although morphosyntactic change due to micro-contact is probably widespread across Italy, it has received almost no attention in the literature. This case study involves the complementizer system of the local language Ferentinese (Southern Lazio), which underwent restructuring over a very brief period. I claim that this change is a case of downward reanalysis from Force to Fin within the split CP, triggered by the regression of the subjunctive and its subsequent replacement by a new complementation strategy. In turn, I argue that this change was the by-product of an increase in the number of complementizers in the language, from two to three, due to micro-contact between Ferentinese and Italiano Regionale. Crucially, the latter furnished a complementizer form (che) identical to one already present in the Ferentinese system, leading to reanalysis. Thus, in addition to reporting on a novel case of micro-contact in Italy, this paper illustrates one pathway to the genesis of a rare three-way complementizer system and sketches an initial typology of how related complementizer systems have changed in diachrony.