bluuebunny/crossref_metadata_2025 huggingface dataset:
Thermal Comfort Conditions and Mortality in Brazil
Authors: Requia Weeberb J. , Damasceno da Silva Reizane Maria , Hoinaski Leonardo , Amini Heresh
Published: September 2024

Conventional temperature-based approaches often overlook the intricate nature of thermal stress experienced by individuals. To address this limitation, climatologists have developed thermal indices—composite measures designed to reflect the complex interaction of meteorological factors influencing human perception of temperature. Our study focuses on Brazil, estimating the association between thermal comfort conditions and mortality related to respiratory and circulatory diseases. We examined four distinct thermal indices: the discomfort index (DI), net effective temperature (NET), humidex (H), and heat index (HI). Analyzing a comprehensive dataset of 2,872,084 deaths from 2003 to 2017, we found significant variation in relative risk (RR) based on health outcomes, exposure lag, percentile of exposure, sex/age groups, and specific thermal indices. For example, under high exposure conditions (99th percentile), we observed that the shorter lags (3, 5, 7, and 10) had the most robust effects on all-cause mortality. For example, under lag 3, the pooled national results for the overall population (all ages and sexes) indicate an increased risk of all-cause mortality, with an RR of 1.17 (95% CI: 1.13; 1.122) for DI, 1.15 (95% CI: 1.12; 1.17) for H, 1.15 (95% CI: 1.09; 1.21) for HI, and 1.18 (95% CI: 1.13; 1.22) for NET. At low exposure levels (1st percentile), all four distinct thermal indices were linked to an increase in all-cause mortality across most sex and age subgroups. Specifically, for lag 20, we observed an estimated RR of 1.19 (95% CI: 1.14; 1.23) for DI, 1.12 (95% CI: 1.08; 1.16) for H, 1.17 (95% CI: 1.12; 1.22) for HI, and 1.18 (95% CI: 1.14; 1.23) for NET. These findings have important implications for policymakers, guiding the development of measures to minimize climate change’s impact on public health in Brazil.
Technology for Young Adults with Stroke: An Australian Environmental Scan
Authors: Amoah Dinah , Prior Sarah , Schmidt Matthew , Mather Carey , Bird Marie-Louise
Published: September 2024

Technology has the potential to address the unique needs of young stroke survivors. Despite this, little is known about the technological resources available to support young adults with stroke. This study aimed to identify and compile available technological resources that cater to the specific needs of young adults (18–30 years) with stroke in Australia. An environmental scan was conducted from December 2023 to March 2024. Sources included websites, app stores, rehabilitation centres, hospitals, organisations, technology developers, and healthcare professionals. Of the 114 resources identified, 11% were for re-training limb movement, 40% for speech rehabilitation, 20% for medication reminders, and 29% were social media posts offering peer mentoring and support. Most limb movement (75%) and medication reminder (87%) apps were free. However, most speech therapy apps (78%) had associated costs. Social media posts were hosted on Facebook (64%), Instagram (21%), TikTok (9%), YouTube (3%), and other websites (3%). Forty-six percent of the social media posts targeting young stroke survivors did not specify the age group. These resources were identified as available to young people with stroke. Although the resources found focused on young stroke survivors, it was difficult to ascertain the specific age group that was being targeted.
Urban–Rural Exposure to Flood Hazard and Social Vulnerability in the Conterminous United States
Authors: Dhungana Bishal , Liu Weibo
Published: September 2024

This study investigates the spatial disparities in flood risk and social vulnerability across 66,543 census tracts in the Conterminous United States (CONUS), emphasizing urban–rural differences. Utilizing the American Community Survey (ACS) 2016–2020 data, we focused on 16 social factors representing socioeconomic status, household composition, racial and ethnic minority status, and housing and transportation access. Principal Component Analysis (PCA) reduced these variables into five principal components: Socioeconomic Disadvantage, Elderly and Disability, Housing Density and Vehicle Access, Youth and Mobile Housing, and Group Quarters and Unemployment. An additive model created a comprehensive Social Vulnerability Index (SVI). Statistical analysis, including the Mann–Whitney U test, indicated significant differences in flood risk and social vulnerability between urban and rural areas. Spatial cluster analysis using Local Indicators of Spatial Association (LISA) revealed significant high flood risk and social vulnerability clusters, particularly in urban regions along the Gulf Coast, Atlantic Seaboard, and Mississippi River. Global and local regression models, including Ordinary Least Squares (OLS) and Geographically Weighted Regression (GWR), highlighted social vulnerability’s spatial variability and localized impacts on flood risk. The results showed substantial regional disparities, with urban areas exhibiting higher flood risks and social vulnerability, especially in southeastern urban centers. The analysis also revealed that Socioeconomic Disadvantage, Group Quarters and Unemployment, and Housing Density and Vehicle Access are closely related to flood risk in urban areas, while in rural areas, the relationship between flood risk and factors such as Elderly and Disability and Youth and Mobile Housing is more pronounced. This study underscores the necessity for targeted, region-specific strategies to mitigate flood risks and enhance resilience, particularly in areas where high flood risk and social vulnerability converge. These findings provide critical insights for policymakers and planners aiming to address environmental justice and promote equitable flood risk management across diverse geographic settings.
miR-10a/b-5p-NCOR2 Regulates Insulin-Resistant Diabetes in Female Mice
Authors: Ha Se Eun , Singh Rajan , Jin Byungchang , Baek Gain , Jorgensen Brian G. , Zogg Hannah , Debnath Sushmita , Park Hahn Sung , Cho Hayeong , Watkins Claudia Marie , Cho Sumin , Kim Min-Seob , Lee Moon Young , Yu Tae Yang , Jeong Jin Woo , Ro Seungil
Published: September 2024

Gender and biological sex have distinct impacts on the pathogenesis of type 2 diabetes (T2D). Estrogen deficiency is known to predispose female mice to T2D. In our previous study, we found that a high-fat, high-sucrose diet (HFHSD) induces T2D in male mice through the miR-10b-5p/KLF11/KIT pathway, but not in females, highlighting hormonal disparities in T2D susceptibility. However, the underlying molecular mechanisms of this hormonal protection in females remain elusive. To address this knowledge gap, we utilized ovariectomized, estrogen-deficient female mice, fed them a HFHSD to induce T2D, and investigated the molecular mechanisms involved in estrogen-deficient diabetic female mice, relevant cell lines, and female T2D patients. Initially, female mice fed a HFHSD exhibited a delayed onset of T2D, but ovariectomy-induced estrogen deficiency promptly precipitated T2D without delay. Intriguingly, insulin (INS) was upregulated, while insulin receptor (INSR) and protein kinase B (AKT) were downregulated in these estrogen-deficient diabetic female mice, indicating insulin-resistant T2D. These dysregulations of INS, INSR, and AKT were mediated by a miR-10a/b-5p-NCOR2 axis. Treatment with miR-10a/b-5p effectively alleviated hyperglycemia in estrogen-deficient T2D female mice, while β-estradiol temporarily reduced hyperglycemia. Consistent with the murine findings, plasma samples from female T2D patients exhibited significant reductions in miR-10a/b-5p, estrogen, and INSR, but increased insulin levels. Our findings suggest that estrogen protects against insulin-resistant T2D in females through miR-10a/b-5p/NCOR2 pathway, indicating the potential therapeutic benefits of miR-10a/b-5p restoration in female T2D management.
Synthesis, Biological Activity, and Molecular-Docking Studies of New Brassinosteroid Analogs
Authors: Nuñez María , Wang Yaowei , Russinova Eugenia , Estévez-Braun Ana , Amesty Angel , Olea Andrés F. , Mellado Marco , Díaz Katy , Espinoza Catalán Luis
Published: September 2024

Much work has been dedicated to the quest to determine the structure–activity relationship in synthetic brassinosteroid (BR) analogs. Recently, it has been reported that analogs with phenyl or benzoate groups in the alkyl chain present activities comparable to those shown by natural BRs, depending on the nature of the substituent in the aromatic ring. However, as it is well known that the activity depends on the structure of the whole molecule, in this work, we have synthesized a series of compounds with the same substituted benzoate in the alkyl chain and a hydroxyl group at C3. The main goal was to compare the activities with analogs with -OH at C2 and C3. Additionally, a molecular-docking study and molecular dynamics simulations were performed to establish a correlation between the experimental and theoretical results. The synthesis of eight new BR analogs was described. All the analogs were fully characterized by spectroscopical methods. The bioactivity of these analogs was assessed using the rice lamina inclination test (RLIT) and the inhibition of the root and hypocotyl elongation of Arabidopsis thaliana. The results of the RLIT indicate that at the lowest tested concentration (1 × 10−8 M), in the BR analogs in which the aromatic ring was substituted at the para position with methoxy, the I and CN substituents were more active than brassinolide (50–72%) and 2–3 times more active than those analogs in which the substituent group was F, Cl or Br atoms. However, at the highest concentrations, brassinolide was the most active compound, and the structure–activity relationship changed. On the other hand, the results of the A. thaliana root sensitivity assay show that brassinolide and the analogs with I and CN as substituents on the benzoyl group were the most active compounds. These results are in line with those obtained via the RLIT. A comparison of these results with those obtained for similar analogs that had a hydroxyl group at C2 indicates the importance of considering the whole structure. The molecular-docking results indicate that all the analogs adopted a brassinolide-like orientation, while the stabilizing effect of the benzoate group on the interactions with the receptor complex provided energy binding values ranging between −10.17 and −13.17 kcal mol−1, where the analog with a nitrile group was the compound that achieved better contact with the amino acids present in the active site.
MAIT Cells in the Bone Marrow of Patients with Aplastic Anemia
Authors: Vu Lam Quang , Espinoza J. Luis , Nguyen Hoang Thao Giang , Mizuno Shohei , Takami Akiyoshi
Published: September 2024

Mucosal-associated invariant T cells (MAIT cells) are a subset of T cells with innate, effector-like properties that play an essential role in the immune response to microbial infections. In humans, MAIT cells are detectable in the blood, liver, and lungs, but little is known about the frequency of these cells in the bone marrow. Also, the pathogenic role, if any, of MAIT cells in the development of aplastic anemia, a disease with an exquisite origin in the bone marrow, is currently unknown. We investigated the frequency and clinical relevance of bone marrow MAIT cells in a cohort of 14 patients (60.6 ± 23 and 57% women) with aplastic anemia. MAIT cells in the bone marrow samples obtained at diagnosis were evaluated by flow cytometry, and their association with various blood cell parameters and the patients’ clinical features was analyzed. MAIT cells were detectable in the bone marrow of all patients, with considerable variations among them. Bone marrow MAIT cells expressing the activator receptor natural killer group 2D - NKG2D (NKG2D+ MAIT cells) were significantly more abundant in the specimens of the aplastic anemia patients than in patients with bone marrow failure distinct from aplastic anemia. In addition, the NKG2D+ MAIT cells positively correlated with whole blood cell counts (WBC), platelet counts, and neutrophil counts, as well as with various inflammatory markers, including neutrophil-to-lymphocyte rate (NLR), platelet-to-lymphocyte rate (PLR), and systemic inflammatory index (SII). In functional studies, bone marrow CD34+ hematopoietic cells exposed to phytohemagglutinin or bacterial-derived lipopolysaccharide and acetyl-6-formylpterin upregulated MR1 (major histocompatibility complex, class I-related, known to interact with MAIT cells) and MICA/B (MHC class I chain-related gene A, a ligand of NKG2D) proteins on their cell surface, suggesting that under stress conditions, CD34+ hematopoietic cells are more likely to interact with NKG2D+ MAIT cells. In addition, NKG2D+ MAIT cells upregulated perforin and granzyme B in response to their interaction with recombinant MICA protein in vitro. This study reports for the first time the frequency of MAIT cells in the bone marrow of patients with aplastic anemia and assesses the potential implications of these cells in the pathogenesis or progression of aplastic anemia.
Aerobic Exercise Training Protects Against Insulin Resistance, Despite Low-Sodium Diet-Induced Increased Inflammation and Visceral Adiposity
Authors: Del Bianco Vanessa , Ferreira Guilherme da Silva , Bochi Ana Paula Garcia , Pinto Paula Ramos , Rodrigues Letícia Gomes , Furukawa Luzia Naoko Shinohara , Okamoto Maristela Mitiko , Almeida Jaíne Alves , da Silveira Lizandre Keren Ramos , Santos Aritania Sousa , Bispo Kely Cristina Soares , Capelozzi Vera Luiza , Correa-Giannella Maria Lucia , da Silva Alexandre Alves , Velosa Ana Paula Pereira , Nakandakare Edna Regina , Machado Ubiratan Fabres , Teodoro Walcy Paganelli Rosolia , Passarelli Marisa , Catanozi Sergio
Published: September 2024

Dietary sodium restriction increases plasma triglycerides (TG) and total cholesterol (TC) concentrations as well as causing insulin resistance and stimulation of the renin-angiotensin-aldosterone system (RAAS) and the sympathetic nervous system. Stimulation of the angiotensin II type-1 receptor (AT1) is associated with insulin resistance, inflammation, and the inhibition of adipogenesis. The current study investigated whether aerobic exercise training (AET) mitigates or inhibits the adverse effects of dietary sodium restriction on adiposity, inflammation, and insulin sensitivity in periepididymal adipose tissue. LDL receptor knockout mice were fed either a normal-sodium (NS; 1.27% NaCl) or a low-sodium (LS; 0.15% NaCl) diet and were either subjected to AET for 90 days or kept sedentary. Body mass, blood pressure (BP), hematocrit, plasma TC, TG, glucose and 24-hour urinary sodium (UNa) concentrations, insulin sensitivity, lipoprotein profile, histopathological analyses, and gene and protein expression were determined. The results were evaluated using two-way ANOVA. Differences were not observed in BP, hematocrit, diet consumption, and TC. The LS diet was found to enhance body mass, insulin resistance, plasma glucose, TG, LDL-C, and VLDL-TG and reduce UNa, HDL-C, and HDL-TG, showing a pro-atherogenic lipid profile. In periepididymal adipose tissue, the LS diet increased tissue mass, TG, TC, AT1 receptor, pro-inflammatory macro-phages contents, and the area of adipocytes; contrarily, the LS diet decreased anti-inflammatory macrophages, protein contents and the transcription of genes related to insulin sensitivity. The AET prevented insulin resistance, but did not protect against dyslipidemia, adipose tissue pro-inflammatory profile, increased tissue mass, AT1 receptor expression, TG, and TC induced by the LS diet.
Histologic Evaluation of Thyroid Nodules Treated with Thermal Ablation: An Institutional Experience
Authors: Russotto Fernanda , Fiorentino Vincenzo , Pizzimenti Cristina , Micali Marina Gloria , Franchina Mariausilia , Pepe Ludovica , Riganati Giuseppe , Giordano Walter , Magliolo Emilia , Ristagno Serenella , Rossi Esther Diana , Tuccari Giovanni , Martini Maurizio , Ieni Antonio , Fadda Guido
Published: September 2024

Thyroid nodules are a common, benign condition with a higher prevalence in women, individuals with iodine deficiency, and radiation exposure. Treatment options for benign thyroid nodules include pharmaceutical therapy, thyroidectomy, and thermal ablation (TA). TA, including laser ablation (LA), radiofrequency ablation (RFA), and microwave ablation (MWA), is a procedure that uses heat to cause tissue necrosis. It is commonly used for large, firm, benign, non-functioning thyroid nodules that cause severe symptoms or pain when surgery is not recommended or desired. When thyroid nodules do not respond to TA, they undergo surgery to resolve the symptoms and clarify the diagnosis. This study aims to analyze the histological alterations found in surgically excised TA-treated thyroid nodules and to evaluate the morphological criteria of differential diagnosis between benign and malignant nodules, establishing whether the alterations observed on the histological sample are a consequence of TA or indicative of neoplastic disease. For this purpose, the adoption of ancillary methods, such as immunohistochemistry, is fundamental to distinguish the artifacts induced by TA from the typical morphological characteristics of malignant neoplasms.
Galectin-7 Expression in the Placentas of Women with Gestational Diabetes Mellitus
Authors: Seifert Christina Teresa , Unverdorben Laura , Knabl Julia , Hutter Stefan , Keckstein Simon , Schmoeckel Elisa , Kessler Mirjana , Jeschke Udo , Mahner Sven , Kolben Thomas , Ganster Franziska
Published: September 2024

Gestational diabetes mellitus (GDM) is a common condition during pregnancy. The prevalence of GDM is continuously increasing worldwide. Due to accessible diagnostic methods and a clear understanding of risk factors, GDM can be effectively diagnosed and managed. Galectins may influence immunomodulatory and inflammatory processes. This study examines the expression of galectin-7 in the placentas of women with gestational diabetes (GDM), compares it to its expression in healthy pregnancies, and evaluates the associated clinical outcomes. The placentas of 40 healthy women and 40 GDM placentas were included in the cohort. The expression level of galecin-7 was measured in the syncytiotrophoblast (SCT) and in the decidua of the placenta by immunohistochemistry and double immunofluorescence staining. The evaluation was performed by an immunoreactivity score (IRS). The study results show an increased expression of galectin-7 in the SCT and the decidua of GDM placentas as compared to the placentas of the control group. Elevated levels of galectin-7 were observed in both the nucleus and the cytoplasm. This study investigated the hypothesis that galectins are involved in pathophysiological processes of gestational diabetes. Statistical analysis of gene expression patterns confirmed that galectin-7 is indeed upregulated in GDM placentas. Further studies are needed to show the correlation of galectin-7 and the development and maintenance of gestational diabetes mellitus.
Markers of Metabolic Abnormalities in Vitiligo Patients
Authors: Papaccio Federica , Ottaviani Monica , Truglio Mauro , D’Arino Andrea , Caputo Silvia , Pacifico Alessia , Iacovelli Paolo , Di Nardo Anna , Picardo Mauro , Bellei Barbara
Published: September 2024

While vitiligo is primarily caused by melanocyte deficiency or dysfunction, recent studies have revealed a notable prevalence of metabolic syndrome (MetS) among patients with vitiligo. This suggests shared pathogenic features between the two conditions. Individuals with vitiligo often exhibit variations in triglyceride levels, cholesterol, and blood pressure, which are also affected in MetS. Given the similarities in their underlying mechanisms, genetic factors, pro-inflammatory signalling pathways, and increased oxidative stress, this study aims to highlight the common traits between vitiligo and metabolic systemic disorders. Serum analyses confirmed increased low-density lipoprotein (LDL) levels in patients with vitiligo, compared to physiological values. In addition, we reported significant decreases in folate and vitamin D (Vit D) levels. Oxidative stress is one of the underlying causes of the development of metabolic syndromes and is related to the advancement of skin diseases. This study found high levels of inflammatory cytokines, such as interleukin-6 (IL-6) and chemokine 10 (CXCL10), which are markers of inflammation and disease progression. The accumulation of insulin growth factor binding proteins 5 (IGFBP5) and advanced glycation end products (AGEs) entailed in atherosclerosis and diabetes onset, respectively, were also disclosed in vitiligo. In addition, the blood-associated activity of the antioxidant enzymes catalase (Cat) and superoxide dismutase (SOD) was impaired. Moreover, the plasma fatty acid (FAs) profile analysis showed an alteration in composition and specific estimated activities of FAs biosynthetic enzymes resembling MetS development, resulting in an imbalance towards pro-inflammatory n6-series FAs. These results revealed a systemic metabolic alteration in vitiligo patients that could be considered a new target for developing a more effective therapeutic approach.
Hypoxia-Induced Adaptations of N-Glycomes and Proteomes in Breast Cancer Cells and Their Secreted Extracellular Vesicles
Authors: Peng Bojia , Bartkowiak Kai , Song Feizhi , Nissen Paula , Schlüter Hartmut , Siebels Bente
Published: September 2024

The hypoxic tumor microenvironment significantly impacts cellular behavior and intercellular communication, with extracellular vesicles (EVs) playing a crucial role in promoting angiogenesis, metastasis, and host immunosuppression, and presumed cancer progression and metastasis are closely associated with the aberrant surface N-glycan expression in EVs. We hypothesize that hypoxic tumors synthesize specific hypoxia-induced N-glycans in response to or as a consequence of hypoxia. This study utilized nano-LC–MS/MS to integrate quantitative proteomic and N-glycomic analyses of both cells and EVs derived from the MDA-MB-231 breast cancer cell line cultured under normoxic and hypoxic conditions. Whole N-glycome and proteome profiling revealed that hypoxia has an impact on the asparagine N-linked glycosylation patterns and on the glycolysis/gluconeogenesis proteins in cells in terms of altered N-glycosylation for their adaptation to low-oxygen conditions. Distinct N-glycan types, high-mannose glycans like Man3 and Man9, were highly abundant in the hypoxic cells. On the other hand, alterations in the sialylation and fucosylation patterns were observed in the hypoxic cells. Furthermore, hypoxia-induced EVs exhibit a signature consisting of mono-antennary structures and specific N-glycans (H4N3F1S2, H3N3F1S0, and H7N4F3S2; H8N4F1S0 and H8N6F1S2), which are significantly associated with poor prognoses for breast tumors, presumably altering the interactions within the tumor microenvironment to promote tumorigenesis and metastasis. Our findings provide an overview of the N-glycan profiles, particularly under hypoxic conditions, and offer insights into the potential biomarkers for tracking tumor microenvironment dynamics and for developing precision medicine approaches in oncology.
Multistimuli Luminescence and Anthelmintic Activity of Zn(II) Complexes Based on 1H-Benzimidazole-2-yl Hydrazone Ligands
Authors: Gusev Alexey , Braga Elena , Kaleukh Alexandr , Baevsky Michail , Kiskin Mikhail , Linert Wolfgang
Published: September 2024

Three novel Zn(II) mononuclear complexes with the general formula ZnL2Cl2 (L = 2-(4-R-phenylmethylene)benzimidazol-2-hydrazines; R-H (1), R-CH3 (2), and R-OCH3 (3)) were synthesized and fully characterized by various means. These complexes demonstrate excitation-dependent emission, which is detected by a change in the emission color (from blue to green) upon an increase in the excitation wavelength. Moreover complex 1 shows reversible mechanochromic luminescence behavior due to the reversible loss of solvated methanol molecules upon the intense grinding of crystals. In addition, 1 exhibits vapochromic properties, which originate from the adsorption methanol vapor on the crystal surface. The strengthening of anthelmintic activity at the transition from free hydrazones to zinc-based complexes is shown.
Investigation of Mechanical and Corrosion Properties of New Mg-Zn-Ga Amorphous Alloys for Biomedical Applications
Authors: Bazhenov Viacheslav E. , Gorobinskiy Mikhail V. , Bazlov Andrey I. , Bautin Vasiliy A. , Koltygin Andrey V. , Komissarov Alexander A. , Ten Denis V. , Li Anna V. , Drobyshev Alexey Yu. , Kang Yoongu , Jung In-Ho , Shin Kwang Seon
Published: September 2024

Magnesium alloys are considered as promising materials for use as biodegradable implants due to their biocompatibility and similarity to human bone properties. However, their high corrosion rate in bodily fluids limits their use. To address this issue, amorphization can be used to inhibit microgalvanic corrosion and increase corrosion resistance. The Mg-Zn-Ga metallic glass system was investigated in this study, which shows potential for improving the corrosion resistance of magnesium alloys for biodegradable implants. According to clinical tests, it has been demonstrated that Ga ions are effective in the regeneration of bone tissue. The microstructure, phase composition, and phase transition temperatures of sixteen Mg-Zn-Ga alloys were analyzed. In addition, a liquidus projection of the Mg-Zn-Ga system was constructed and validated through the thermodynamic calculations based on the CALPHAD-type database. Furthermore, amorphous ribbons were prepared by rapid solidification of the melt for prospective alloys. XRD and DSC analysis indicate that the alloys with the most potential possess an amorphous structure. The ribbons exhibit an ultimate tensile strength of up to 524 MPa and a low corrosion rate of 0.1–0.3 mm/year in Hanks’ solution. Therefore, it appears that Mg-Zn-Ga metallic glass alloys could be suitable for biodegradable applications.
Efficient End-to-End Convolutional Architecture for Point-of-Gaze Estimation
Authors: Miron Casian , Ciubotariu George , Păsărică Alexandru , Timofte Radu
Published: September 2024

Point-of-gaze estimation is part of a larger set of tasks aimed at improving user experience, providing business insights, or facilitating interactions with different devices. There has been a growing interest in this task, particularly due to the need for upgrades in e-meeting platforms during the pandemic when on-site activities were no longer possible for educational institutions, corporations, and other organizations. Current research advancements are focusing on more complex methodologies for data collection and task implementation, creating a gap that we intend to address with our contributions. Thus, we introduce a methodology for data acquisition that shows promise due to its nonrestrictive and straightforward nature, notably increasing the yield of collected data without compromising diversity or quality. Additionally, we present a novel and efficient convolutional neural network specifically tailored for calibration-free point-of-gaze estimation that outperforms current state-of-the-art methods on the MPIIFaceGaze dataset by a substantial margin, and sets a strong baseline on our own data.
Double Anterior Interventricular Arteries: Prevalence and Morphological Types—A Dissection Study
Authors: Daescu Ecaterina , Enache Alexandra , Stan Emanuela , Bolintineanu Sorin Lucian , Ghenciu Laura Andreea , Faur Alexandra Corina , Pusztai Agneta Maria , Zahoi Delia Elena
Published: September 2024

Background: This study aimed to evaluate the prevalence of double anterior interventricular artery using the dissection method. Metode: A retrospective study was conducted between 2010 and 2024 at the Anatomy and Embryology Laboratory of the Victor Babes University of Medicine and Pharmacy in Timisoara. Eighty cases were analyzed for morphological variants of the coronary arteries, especially the anterior interventricular artery. Results: Two cases of double anterior interventricular arteries were identified. In the first case, the two anterior interventricular arteries originated from the anterior interventricular branch of the left coronary artery. In the second case, an additional anterior interventricular artery with an aortic origin was found running along the lower third of the two interventricular grooves. This shape has not been described before in the specialized literature. Conclusions: Knowing the potential variations of the double left anterior descending artery is critical for interpreting cardiac imaging and choosing and planning percutaneous and surgical reperfusion strategies.
Melatonin Receptors and Serotonin: Age-Related Changes in the Ovaries
Authors: Polyakova Victoria , Medvedev Dmitrii , Linkova Natalia , Mushkin Mikhail , Muraviev Alexander , Krasichkov Alexander , Dyatlova Anastasiia , Ivanova Yanina , Gullo Giuseppe , Gorelova Anna Andreevna
Published: September 2024

Introduction. Melatonin and serotonin can influence certain aging processes in the ovaries. The main melatonin receptors are represented by types MT1 and MT2. The goal of investigation. Here, we evaluated the expression of genes and synthesis of MT1 and MT2 receptors, as well as serotonin synthesis in the ovaries during ontogenesis. Methods. We analyzed histological material obtained from the ovaries of infants, women of younger and older reproductive age, premenopausal, menopausal, and postmenopausal women. For the analysis of MT1 and MT2 receptors and serotonin expression and synthesis, RT-PCR and immunohistochemistry were used. Results. We found that the synthesis of serotonin, as well as MT1 and MT2 receptors in the ovaries significantly decrease in ontogenesis. The sharpest drop in these molecules was observed in samples obtained from one-year-old infants, as well as from pubescent girls and menopausal women. A statistically significant 2.3–7.6-fold decrease in the expression of MTNR1A and MTNR1B genes in the ovaries was also observed in one-year-old infants, in adolescents, and in middle-aged women. Conclusions. These data are crucial to understanding the fundamental mechanisms of aging of the female reproductive system and the search for molecules predicting its aging.
Clinical and Laboratory Parameters Associated with PICU Admission in Children with Multisystem Inflammatory Syndrome Associated with COVID-19 (MIS-C)
Authors: Dourdouna Maria-Myrto , Mpourazani Evdoxia , Tatsi Elizabeth-Barbara , Tsirogianni Chrysanthi , Barbaressou Charikleia , Dessypris Nick , Michos Athanasios
Published: September 2024

Background/Objectives: Multisystem Inflammatory Syndrome in children (MIS-C) is a rare but severe post-infectious complication of COVID-19 that often requires admission to the Pediatric Intensive Care Unit (PICU). The present study aimed to compare the demographic, clinical, and laboratory characteristics of children diagnosed with MIS-C who were admitted to the PICU and those who did not require PICU admission. Methods: Children diagnosed with MIS-C from September 2020 to April 2023 were included in this case-control study. Demographic, clinical, and laboratory data were collected from medical records. Results: Fifty children with MIS-C were included in the study [median (IQR) age: 7.5 (4.3, 11.4) years, 28/50 (56%) males]. Twenty-two (22/50, 44%) children required admission to the PICU. In the multivariate regression analysis, hepatic (OR: 12.89, 95%CI: 1.35–123.41, p-value = 0.03) and cardiological involvement (OR: 34.55, 95%CI: 2.2–541.91, p-value = 0.01) were significantly associated with hospitalization at the PICU. Regarding the laboratory and imaging parameters during the first 48 h from admission, D-dimer levels higher than 4 μg/mL and decreased Left Ventricular Ejection Fraction (LVEF) were associated with an increased risk of PICU admission (OR: 7.95, 95%CI: 1.48–42.78, p-value = 0.02 and OR = 1.28, 95%CI: 1.07–1.53, p-value = 0.01). Children who were admitted to the PICU were more likely to develop complications during their hospitalization (10/22, 45.5% vs. 3/28, 10.7%, p-value = 0.005) and were hospitalized for more days than children in the pediatric ward (median length of stay (IQR): 20 (15, 28) days vs. 8.5 (6, 14) days, p-value < 0.001). Conclusions: The findings of this study indicate that cardiovascular and hepatic involvement and increased D-dimer levels in children with MIS-C might be associated with admission to the PICU.
The Impact of Urbanization on Surface Runoff and Flood Prevention Strategies: A Case Study of a Traditional Village
Authors: Li Jiaxin , Zhou Wuzhong , Tao Cong
Published: September 2024

Increasingly severe flooding disasters have caused heavy casualties and property losses worldwide. Traditional Chinese villages that rarely experienced flooding disasters in the past have begun to frequently suffer from floods due to unreasonable reconstruction activities such as ground hardening and pond filling caused by urbanization. However, previous studies on hydrological changes and flood disasters caused by reconstruction activities in rural areas are scarce, especially lacking in quantitative analysis and research. In this study, the Storm Water Management Model (SWMM) is used to construct two hydrological models before and after the reconstruction of Hezhai Village, a traditional Chinese village. By simulating and comparing the changes in hydrological indicators of the two models, this study quantitatively analyzes how reconstruction activities caused changes in surface runoff and flooding disasters in Hezhai Village. The results show that the increase in the impervious ratio in the village has obvious effects on the total runoff, peak runoff, and runoff coefficient. And the reconstruction of ponds and canals has a notable impact on flooding. This study further delves into the logic of flooding at ponds and ordinary nodes and analyzes the specific reasons for flooding in Hezhai Village. Based on this, the paper provides recommendations for the optimization of the reconstruction of Hezhai Village.
Leveraging Greenspace to Manage Urban Flooding: An Investigation of Nature-Based Solutions Implementation in U.S. Public Parks
Authors: Li Jiayang , Guo Ziyi
Published: September 2024

Many cities are looking to adopt nature-based solutions (NBS) in greenspace to manage urban flooding and provide diverse co-benefits. Yet little research exists to inform the planning and design of park NBS. This study investigated NBS adoption in 58 public parks across major U.S. cities, using a 2022 survey by the Trust for Public Lands and other secondary datasets. We developed a typology to conceptualize a wide range of park NBS into five high-level categories by size/capacity, location of the gray–green spectrum, and design objectives. We then employed this typology to explore how a park’s adopted NBS types may relate to its landscape and sociodemographic contexts. We found that the most used type of NBS in the studied parks was ECO (a typology we defined as conserving, restoring, or creating ecosystems to mitigate flooding through ecological processes and functions), while the least used NBS type was ENG (a typology we defined as imitating natural infiltration processes but having no living elements). Further, parks that adopted ECO had significantly higher percentages of greenspace in the surrounding, as well as higher flood risks. We also found notable—though not statistically significant—evidence of potential associations between the type of NBS implementation in a park and its nearby neighborhoods’ income level, poverty, and population racial and age compositions. Moreover, our findings indicated that park visitors were more privileged compared to residents living near a park. We concluded that park contextual factors deserve more explicit consideration in the planning and design of NBS and discussed key implications of this study for practice and future research around park NBS for urban flooding.
Response of Hydrothermal Conditions to the Saturation Values of Forest Aboveground Biomass Estimation by Remote Sensing in Yunnan Province, China
Authors: Wu Yong , Guo Binbing , Zhang Xiaoli , Luo Hongbin , Yu Zhibo , Li Huipeng , Shi Kaize , Wang Leiguang , Xu Weiheng , Ou Guanglong
Published: September 2024

Identifying the key climate variables affecting optical saturation values (OSVs) in forest aboveground biomass (AGB) estimation using optical remote sensing is crucial for analyzing OSV changes. This can improve AGB estimation accuracy by addressing the uncertainties associated with optical saturation. In this study, Pinus yunnanensis forests and Landsat 8 OLI imagery from Yunnan were used as case studies to explain this issue. The spherical model was applied to determine the OSVs using specific spectral bands (Blue, Green, Red, Near-Infrared (NIR), and Short-Wave Infrared Band 2 (SWIR2)) derived from Landsat 8 OLI imagery. Canonical correlation analysis (CCA) uncovered the intricate relationships between climatic variables and OSV variations. The results reveal the following: (1) All Landsat 8 OLI spectral bands showed a negative correlation with the Pinus yunnanensis forest AGB, with OSVs ranging from 104.42 t/ha to 209.11 t/ha, peaking in the southwestern region and declining to the lowest levels in the southeastern region. (2) CCA effectively explained 93.2% of the OSV variations, identifying annual mean temperature (AMT) as the most influential climatic factor. Additionally, the mean temperature of the wettest quarter (MTQ) and annual precipitation (ANP) were significant secondary determinants, with higher OSV values observed in warmer, more humid areas. These findings offer important insights into climate-driven OSV variations, reducing uncertainty in forest AGB estimation and enhancing the precision of AGB estimations in future research.
Analysis Methods for Landscapes and Features of Traditional Villages Based on Digital Technology—The Example of Puping Village in Zhangzhou
Authors: Wang Liangliang , Wang Yixin , Huang Wencan , Han Jie
Published: September 2024

Many traditional villages have been degraded to a certain extent due to urbanization and out-of-control management. In addition, due to the lack of recognition and continuation of spatial texture in some village conservation and planning that, in turn, resulting in the gradual disappearance of their distinctive landscape feature. Studying the spatial form of traditional villages helps preserve the authenticity of traditional villages as cultural landscape and inherits traditional historical characteristics. Using Puping Village in Zhangzhou City, Fujian Province as an example, this paper obtains the integrated information data of the village through UAV oblique photography, classifies and extracts the spatial constitution of the traditional village using digital technology, quantitatively analyses it from macroscopic to microscopic, and summarizes the spatial morphology analysis method of the traditional village. The results demonstrate that digital technology can effectively and accurately complete data collection and can provide an objective basis for zoning conservation of traditional villages based on the distinction between new and historic buildings. In addition, digital information collection on the traditional villages landscape features will prepare for the establishment of a database and comparative analysis in the future. We further suggest that digital technology analysis needs to be combined with traditional methods to have a deeper understanding of the formation process of village spatial morphology. The results of the practice in Puping Village show that the use of digital technology can provide a scientific basis for the protection and planning of traditional villages, and that this method is adaptable, which can help to efficiently collect and analyze data on landscape characteristics of other similar villages in China, and support innovative methodologies and technologies for China’s rural revitalization efforts.
Comparative Discourse Strategies in Environmental Advocacy: Analysing the Rhetoric of Greta Thunberg and Chris Packham
Authors: Ponton Douglas Mark , Raimo Anna
Published: September 2024

This paper examines the rhetoric and argumentation of two prominent environmental activists, Greta Thunberg and Chris Packham. From the perspective of Ecolinguistics, Thunberg has given voice to a generational movement for change, galvanising young people everywhere through high-profile protests and speeches. Packham represents British mainstream environmentalism, notably as the presenter of the acclaimed nature documentary ‘Springwatch’. We argue that their influence partially stems from their alignment with dominant cultural narratives: Thunberg’s emphasis on intergenerational discord and Packham’s connection to the natural world. We analyse both figures via the lens of the ‘emotionalisation of media discourse’, highlighting argumentation strategies that feature expressions of negative emotions of which anger is a type. Thunberg’s famous ‘How dare you?’ outburst at the 2019 UN Climate Action Summit and Packham’s statement to Novara Media, ‘I am more angry now than at any point since my 20s’, exemplify this trend. We explore the pragmatic implications of their argumentative and discursive strategies, suggesting that while both have significantly elevated the profile of ecological activism, their discourse may also have a potentially divisive aspect.
The Prevalence of Demodex spp. Infestation in Dermatological Patients in Northern Poland
Authors: Rychlik Katarzyna , Sternicka Julia , Zabłotna Monika , Nowicki Roman J. , Bieniaszewski Leszek , Purzycka-Bohdan Dorota
Published: September 2024

The presence of Demodex spp. mites on the skin is a common phenomenon in the human population. In most people, it is an asymptomatic infestation, but in some patients, it can contribute to the occurrence of diseases such as demodicosis, rosacea, or blepharitis, as well as non-specific symptoms. In this study, the results of tests assessing the presence of Demodex spp. by direct microscopic examination (DME) in 2508 patients from northern Poland with the suspicion of demodicosis were retrospectively analyzed. A total of 3684 tests were performed. Among them, only 1585 (43.02%) revealed the presence of Demodex spp., while 2099 (56.98%) were negative. It was shown that in the analyzed population of patients with clinical suspicion of demodicosis, the degree of confirmation of the presence of Demodex spp. positively correlated with the patient’s age (p = 0.001) and the mite was mainly found on the edges of eyelids and on the facial skin. Additionally, in men, the presence of Demodex was more often confirmed than in women (p = 0.004). In conclusion, the proper diagnosis of demodicosis still constitutes an important clinical problem due to the non-specificity of symptoms and the low confirmation of clinical suspicions of infestation by DME, especially in lower age groups.
The Role of Digital Dermoscopy and Follow-Up in the Detection of Amelanotic/Hypomelanotic Melanoma in a Group of High-Risk Patients—Is It Useful?
Authors: Jurakić Tončić Ružica , Vasari Lara , Štulhofer Buzina Daška , Ledić Drvar Daniela , Petković Mikela , Čeović Romana
Published: September 2024

The prognosis, outcome, and overall survival of melanoma patients improve with early diagnosis which has been facilitated in the past few decades with the introduction of dermoscopy. Further advancements in dermoscopic research, coupled with skilled, educated dermatologists in dermoscopy, have contributed to timely diagnoses. However, detecting amelanotic and hypomelanotic melanoma remains a challenge even to the most skilled experts because these melanomas can mimic inflammatory diseases, numerous benign lesions, and non-melanoma skin cancers. The list of the possible differential diagnoses can be long. Melanoma prediction without the pigment relies only on vascular criteria, and all classic dermoscopic algorithms have failed to fulfill our expectations. In fact, the diagnosis of amelanotic and hypomelanotic melanomas is very challenging, which is why every tool in detecting these lesions is of significance. This review aims to explore the current knowledge and the literature on the possibility of detecting amelanotic/hypomelanotic melanomas using sequential monitoring with digital dermoscopy and total body skin photography.
Avoided and Avoidable Deaths with the Use of COVID-19 Convalescent Plasma in Italy during the First Two Years of Pandemic
Authors: Franchini Massimo , Casadevall Arturo , Dragotakes Quigly , Focosi Daniele
Published: September 2024

Italy was the first western country to be hit by the COVID-19 pandemic and has suffered nearly 200,000 deaths so far during the four years of the pandemic. In March 2020, Italy first deployed COVID-19 convalescent plasma (CCP) to treat hospitalized patients. Despite this initial effort, the proportion of COVID-19 patients treated with CCP during the first two years of the pandemic (2020–2021) was very low (approximately 2% of individuals hospitalized for COVID-19). In this study, we estimated the number of actual inpatient lives saved by CCP treatment in Italy using national mortality data, and CCP mortality reduction data from meta-analyses of randomized controlled trials and real-world data. We also estimated the potential number of lives saved if CCP had been deployed to 100% of hospitalized patients or used in 15% to 75% of outpatients. According to these models, CCP usage in 2020–2021 saved between 385–1304 lives, but this number would have increased to 17,751–60,079 if 100% of inpatients had been transfused with CCP. Similarly, broader (15–75%) usage in outpatients could have prevented 21,187–190,689 hospitalizations (desaturating hospitals) and 6144–81,926 deaths. These data have important implications for convalescent plasma use in future infectious disease emergencies.
Effect of Substrate Bias on the Structure and Tribological Performance of (AlTiVCrNb)CxNy Coatings Deposited via Graphite Co-Sputtering
Authors: Cai Haichao , Guo Pengge , Xue Yujun , Pei Lulu , Zhang Yinghao , Ye Jun
Published: September 2024

In the existing literature, there are few studies on the effect of deposition bias on the tribological properties of carbon-doped high-entropy alloy coatings. In order to further study the effect of the deposition bias on the properties of coatings, (AlTiVCrNb)CxNy coatings were deposited via unbalanced RF magnetron sputtering. The microstructure and tribological properties of carbon-doped high-entropy alloy ceramic coatings under different deposition biases were studied. The composition, morphology, crystal structure, and chemical morphology of each element of the coating were analyzed using scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The hardness, elastic modulus, friction, and wear properties of the coating were further characterized using a nanoindentation instrument, reciprocating sliding friction, a wear tester, and a white light interferometer. The coating density reached the optimal level when the deposition bias value was 90 V. The hardness and elastic modulus of the (AlTiVCrNb)CxNy coating increased first and then decreased with an increase in deposition bias, and the maximum hardness was 23.98 GPa. When the deposition bias was 90 V, the coating formed a good-quality carbon transfer film on the surface of the counterbody due to sp2 clusters during the friction and wear process. The average friction coefficient and wear rate of the (AlTiVCrNb)CxNy coating were the lowest, 0.185 and 1.6 × 10−7 mm3/N·m, respectively. The microstructure, mechanical properties, and tribological performance of the (AlTiVCrNb)CxNy coating were greatly affected by the change in deposition bias, and an (AlTiVCrNb)CxNy coating with excellent structure and friction properties could be prepared using graphite co-sputtering.