bluuebunny/crossref_metadata_2025 huggingface dataset:
Abnormal Calcium Handling in Duchenne Muscular Dystrophy: Mechanisms and Potential Therapies
Authors: Mareedu Satvik , Million Emily D. , Duan Dongsheng , Babu Gopal J.
Published: April 2021

Duchenne muscular dystrophy (DMD) is an X-linked muscle-wasting disease caused by the loss of dystrophin. DMD is associated with muscle degeneration, necrosis, inflammation, fatty replacement, and fibrosis, resulting in muscle weakness, respiratory and cardiac failure, and premature death. There is no curative treatment. Investigations on disease-causing mechanisms offer an opportunity to identify new therapeutic targets to treat DMD. An abnormal elevation of the intracellular calcium (Cai2+) concentration in the dystrophin-deficient muscle is a major secondary event, which contributes to disease progression in DMD. Emerging studies have suggested that targeting Ca2+-handling proteins and/or mechanisms could be a promising therapeutic strategy for DMD. Here, we provide an updated overview of the mechanistic roles the sarcolemma, sarcoplasmic/endoplasmic reticulum, and mitochondria play in the abnormal and sustained elevation ofCai2+levels and their involvement in DMD pathogenesis. We also discuss current approaches aimed at restoring Ca2+homeostasis as potential therapies for DMD.
Locust Hemolymph Conveys Erythropoietin-Like Cytoprotection via Activation of the Cytokine Receptor CRLF3
Authors: Knorr Debbra Y. , Hartung Denise , Schneider Kristin , Hintz Luzia , Pies Hanna S. , Heinrich Ralf
Published: April 2021

The cytokine receptor-like factor 3 (CRLF3) is an evolutionary conserved class 1 cytokine receptor present in all major eumetazoan groups. Endogenous CRLF3 ligands have not been identified and the physiological responses mediated by mammalian CRLF3 are poorly characterized. Insect CRLF3 is activated by erythropoietin (Epo) and several related molecules that protect mammalian neurons from stress-induced apoptosis. However, insects neither express Epo nor “classical” Epo receptor. Cell-protective effects of insect hemolymph have been described for several species. In this study, we explored the possibility that the endogenous CRLF3 ligand is contained in locust hemolymph. PCR analyses confirmed expression ofcrfl3-transcripts in neurons and hemocytes ofLocusta migratoriaandTribolium castaneum. Survival of locust hemocytes in primary cultures was significantly increased by supplementation of culture medium with locust hemolymph serum. Locust primary neuron cultures were also protected by locust hemolymph, though preceding exposure to fetal bovine serum changed the hemolymph dose-dependency of neuroprotection. Direct comparison of 10% hemolymph serum with recombinant human Epo in its optimal neuroprotective concentration revealed equivalent anti-apoptotic effects on hypoxia-exposed locust neurons. The same concentration of locust hemolymph serum also protected hypoxia-exposedT. castaneumneurons. This indicates that the neuroprotective factor in locust hemolymph is sufficiently conserved in insects to allow activation of neuroprotective receptors in different species. Locust hemolymph-induced neuroprotection in bothL. migratoriaandT. castaneumwas abolished after RNAi-mediated suppression ofcrlf3-expression. In summary, we report the presence of a conserved endogenous cytokine in locust hemolymph that activates CRLF3 and connected anti-apoptotic processes in hemocytes and neurons. Identification and characterization of the CRLF3 ligand will promote knowledge about cytokine evolution and may unravel cell-protective agents with potential clinical application.
A Computational Study of the Effects of Tachycardia-Induced Remodeling on Calcium Wave Propagation in Rabbit Atrial Myocytes
Authors: Vagos Márcia R. , Arevalo Hermenegild , Heijman Jordi , Schotten Ulrich , Sundnes Joakim
Published: April 2021

In atrial cardiomyocytes without a well-developed T-tubule system, calcium diffuses from the periphery toward the center creating a centripetal wave pattern. During atrial fibrillation, rapid activation of atrial myocytes induces complex remodeling in diffusion properties that result in failure of calcium to propagate in a fully regenerative manner toward the center; a phenomenon termed “calcium silencing.” This has been observed in rabbit atrial myocytes after exposure to prolonged rapid pacing. Although experimental studies have pointed to possible mechanisms underlying calcium silencing, their individual effects and relative importance remain largely unknown. In this study we used computational modeling of the rabbit atrial cardiomyocyte to query the individual and combined effects of the proposed mechanisms leading to calcium silencing and abnormal calcium wave propagation. We employed a population of models obtained from a newly developed model of the rabbit atrial myocyte with spatial representation of intracellular calcium handling. We selected parameters in the model that represent experimentally observed cellular remodeling which have been implicated in calcium silencing, and scaled their values in the population to match experimental observations. In particular, we changed the maximum conductances of ICaL, INCX, and INaK, RyR open probability, RyR density, Serca2a density, and calcium buffering strength. We incorporated remodeling in a population of 16 models by independently varying parameters that reproduce experimentally observed cellular remodeling, and quantified the resulting alterations in calcium dynamics and wave propagation patterns. The results show a strong effect of ICaL in driving calcium silencing, with INCX, INaK, and RyR density also resulting in calcium silencing in some models. Calcium alternans was observed in some models where INCX and Serca2a density had been changed. Simultaneously incorporating changes in all remodeled parameters resulted in calcium silencing in all models, indicating the predominant role of decreasing ICaL in the population phenotype.
Dietary Exposure to Low Levels of Crude Oil Affects Physiological and Morphological Phenotype in Adults and Their Eggs and Hatchlings of the King Quail (Coturnix chinensis)
Authors: Bautista Naim M. , do Amaral-Silva Lara , Dzialowski Edward , Burggren Warren W.
Published: April 2021

Despite the current knowledge of the devastating effects of external exposure to crude oil on animal mortality, the study of developmental, transgenerational effects of such exposure has received little attention. We used the king quail as an animal model to determine if chronic dietary exposure to crude oil in a parental population would affect morpho-physiological phenotypic variables in their immediate offspring generation. Adult quail were separated into three groups: (1) Control, and two experimental groups dietarily exposed for at least 3 weeks to (2) Low (800 PAH ng/g food), or (3) High (2,400 PAH ng/g food) levels of crude oil. To determine the parental influence on their offspring, we measured metabolic and respiratory physiology in exposed parents and in their non-exposed eggs and hatchlings. Body mass and numerous metabolic (e.g., O2consumption, CO2production) and respiratory (e.g., ventilation frequency and volume) variables did not vary between control and oil exposed parental groups. In contrast, blood PO2, PCO2, and SO2varied among parental groups. Notably, water loss though the eggshell was increased in eggs from High oil level exposed parents. Respiratory variables of hatchlings did not vary between populations, but hatchlings obtained from High oil-exposed parents exhibited lower capacities to maintain body temperature while exposed to a cooling protocol in comparison to hatchlings from Low- and Control-derived parents. The present study demonstrates that parental exposure to crude oil via diet impacts some aspects of physiological performance of the subsequent first (F1) generation.
Cotranscriptional and Posttranscriptional Features of the Transcriptome in Soybean Shoot Apex and Leaf
Authors: Zhu Jiafu , Zhao Han , Kong Fanjiang , Liu Baohui , Liu Min , Dong Zhicheng
Published: April 2021

Transcription is the first step of central dogma, in which the genetic information stored in DNA is copied into RNA. In addition to mature RNA sequencing (RNA-seq), high-throughput nascent RNA assays have been established and applied to provide detailed transcriptional information. Here, we present the profiling of nascent RNA from trifoliate leaves and shoot apices of soybean. In combination with nascent RNA (chromatin-bound RNA, CB RNA) and RNA-seq, we found that introns were largely spliced cotranscriptionally. Although alternative splicing (AS) was mainly determined at nascent RNA biogenesis, differential AS between the leaf and shoot apex at the mature RNA level did not correlate well with cotranscriptional differential AS. Overall, RNA abundance was moderately correlated between nascent RNA and mature RNA within each tissue, but the fold changes between the leaf and shoot apex were highly correlated. Thousands of novel transcripts (mainly non-coding RNA) were detected by CB RNA-seq, including the overlap of natural antisense RNA with two important genes controlling soybean reproductive development, FT2a and Dt1. Taken together, we demonstrated the adoption of CB RNA-seq in soybean, which may shed light on gene expression regulation of important agronomic traits in leguminous crops.
Biopesticide Trunk Injection Into Apple Trees: A Proof of Concept for the Systemic Movement of Mint and Cinnamon Essential Oils
Authors: Werrie Pierre-Yves , Burgeon Clément , Le Goff Guillaume Jean , Hance Thierry , Fauconnier Marie-Laure
Published: April 2021

The use of conventional pesticides is debated because of their multiple potential adverse effects on non-target organisms, human health, pest resistance development and environmental contaminations. In this setting, this study focused on developing alternatives, such as trunk-injected essential oil (EO)-based biopesticides. We analysed the ecophysiology of apple trees (Malus domestica) following the injection ofCinnamomum cassiaandMentha spicatananoemulsions in the tree’s vascular system. Targeted and untargeted volatile organic compounds (VOCs) analyses were performed on leaf-contained and leaf-emitted VOCs and analysed through dynamic headspace–gas chromatography–mass spectrometry (DHS-GC-MS) and thermal desorption unit (TDU)-GC-MS. Our results showed that carvone, as a major constituent of theM. spicataEO, was contained in the leaves (mean concentrations ranging from 3.39 to 19.7 ng gDW–1) and emitted at a constant rate of approximately 0.2 ng gDW–1h–1.Trans-cinnamaldehyde,C. cassia’s major component, accumulated in the leaves (mean concentrations of 83.46 and 350.54 ng gDW–1) without being emitted. Furthermore, our results highlighted the increase in various VOCs following EO injection, both in terms of leaf-contained VOCs, such as methyl salicylate, and in terms of leaf-emitted VOCs, such as caryophyllene. Principal component analysis (PCA) highlighted differences in terms of VOC profiles. In addition, an analysis of similarity (ANOSIM) and permutational multivariate analysis of variance (PERMANOVA) revealed that the VOC profiles were significantly impacted by the treatment. Maximum yields of photosystem II (Fv/Fm) were within the range of 0.80–0.85, indicating that the trees remained healthy throughout the experiment. Our targeted analysis demonstrated the systemic translocation of EOs through the plant’s vascular system. The untargeted analysis, on the other hand, highlighted the potential systemic acquired resistance (SAR) induction by these EOs. Lastly,C. cassiaandM. spicataEOs did not appear phytotoxic to the treated trees, as demonstrated through chlorophyll fluorescence measurements. Hence, this work can be seen as a proof of concept for the use of trunk-injected EOs given the systemic translocation, increased production and release of biogenic VOCs (BVOCs) and absence of phytotoxicity. Further works should focus on the ecological impact of such treatments in orchards, as well as apple quality and production yields.
An R2R3-MYB Transcription Factor Positively Regulates the Glandular Secretory Trichome Initiation in Artemisia annua L.
Authors: Qin Wei , Xie Lihui , Li Yongpeng , Liu Hang , Fu Xueqing , Chen Tiantian , Hassani Danial , Li Ling , Sun Xiaofen , Tang Kexuan
Published: April 2021

Artemisia annuaL. is known for its specific product “artemisinin” which is an active ingredient for curing malaria. Artemisinin is secreted and accumulated in the glandular secretory trichomes (GSTs) onA. annualeaves. Earlier studies have shown that increasing GST density is effective in increasing artemisinin content. However, the mechanism of GST initiation is not fully understood. To this end, we isolated and characterized an R2R3-MYB gene,AaMYB17, which is expressed specifically in the GSTs of shoot tips. Overexpression ofAaMYB17inA. annuaincreased GST density and enhanced the artemisinin content, whereas RNA interference ofAaMYB17resulted in the reduction of GST density and artemisinin content. Additionally, neither overexpression lines nor RNAi lines showed an abnormal phenotype in plant growth and the morphology of GSTs. Our study demonstrates that AaMYB17 is a positive regulator of GSTs’ initiation, without influencing the trichome morphology.
You May Have My Help but Not Necessarily My Care: The Effect of Social Class and Empathy on Prosociality
Authors: Jiménez-Moya Gloria , Luengo Kanacri Bernadette Paula , Cumsille Patricio , Martínez M. Loreto , Berger Christian
Published: April 2021

Previous research has focused on the relation between social class and prosocial behavior. However, this relation is yet unclear. In this work, we shed light on this issue by considering the effect of the level of empathy and the social class of the recipient of help on two types of prosociality, namely helping and caring. In one experimental study, we found that for high-class participants, empathy had a positive effect on helping, regardless of the recipient’s social class. However, empathy had no effect for low-class participants. When it comes to caring, empathy had a positive effect for both high and low-class participants, but only when the recipient of help belonged to the same social class. This highlights that empathy by itself is not sufficient to promote cooperative relations and that the social class of the recipient of help should be taken into account to shed light on this issue.
Toddlers’ Ability to Leverage Statistical Information to Support Word Learning
Authors: Ellis Erica M. , Borovsky Arielle , Elman Jeffrey L. , Evans Julia L.
Published: April 2021

Purpose

This study investigated whether the ability to utilize statistical regularities from fluent speech and map potential words to meaning at 18-months predicts vocabulary at 18- and again at 24-months.

Method

Eighteen-month-olds (N = 47) were exposed to an artificial language with statistical regularities within the speech stream, then participated in an object-label learning task. Learning was measured using a modified looking-while-listening eye-tracking design. Parents completed vocabulary questionnaires when their child was 18-and 24-months old.

Results

Ability to learn the object-label pairing for words after exposure to the artificial language predicted productive vocabulary at 24-months and amount of vocabulary change from 18- to 24 months, independent of non-verbal cognitive ability, socio-economic status (SES) and/or object-label association performance.

Conclusion

Eighteen-month-olds’ ability to use statistical information derived from fluent speech to identify words within the stream of speech and then to map the “words” to meaning directly predicts vocabulary size at 24-months and vocabulary change from 18 to 24 months. The findings support the hypothesis that statistical word segmentation is one of the important aspects of word learning and vocabulary acquisition in toddlers.
Spatial and Motor Aspects in the “Action-Sentence Compatibility Effect”
Authors: Greco Alberto
Published: April 2021

The Action-sentence Compatibility Effect (ACE) is often taken as supporting the fundamental role of the motor system in understanding sentences that describe actions. This effect would be related to an internal “simulation,” i.e., the reactivation of past perceptual and motor experiences. However, it is not easy to establish whether this simulation predominantly involves spatial imagery or motor anticipation. In the classical ACE experiments, where a real motor response is required, the direction and motor representations are mixed. In order to disentangle spatial and motor aspects involved in the ACE, we performed six experiments in different conditions, where the motor component was always reduced, asking participants to judge the sensibility of sentences by moving a mouse, thus requiring a purely spatial representation, compatible with nonmotor interpretations. In addition, our experiments had the purpose of taking into account the possible confusion of effects of practice and of compatibility (i.e., differences in reaction times simultaneously coming from block order and opposite motion conditions). Also, in contrast to the usual paradigm, we included no-transfer filler sentences in the analysis. The ACE was not found in any experiment, a result that failed to support the idea that the ACE could be related to a simulation where spatial aspects rather than motor ones prevail. Strong practice effects were always found and were carved out from results. A surprising effect was that no-transfer sentences were processed much slower than others, perhaps revealing a sort of participants’ awareness of the structure of stimuli, i.e., their finding that some of them involved motion and others did not. The relevance of these outcomes for the embodiment theory is discussed.
Behavioral Restriction Determines Left Attentional Bias: Preliminary Evidences From COVID-19 Lockdown
Authors: Lardone Anna , Turriziani Patrizia , Sorrentino Pierpaolo , Gigliotta Onofrio , Chirico Andrea , Lucidi Fabio , Mandolesi Laura
Published: April 2021

During the COVID-19 lockdown, individuals were forced to remain at home, hence severely limiting the interaction within environmental stimuli, reducing the cognitive load placed on spatial competences. The effects of the behavioral restriction on cognition have been little examined. The present study is aimed at analyzing the effects of lockdown on executive function prominently involved in adapting behavior to new environmental demands. We analyze non-verbal fluency abilities, as indirectly providing a measure of cognitive flexibility to react to spatial changes. Sixteen students (mean age 20.75; SD 1.34), evaluated before the start of the lockdown (T1) in a battery of psychological tasks exploring different cognitive domains, have been reassessed during lockdown (T2). The assessment included the modified Five-Point Test (m-FPT) to analyze non-verbal fluency abilities. At T2, the students were also administered the Toronto Alexithymia Scale (TAS-20). The restriction of behaviors following a lockdown determines increased non-verbal fluency, evidenced by the significant increase of the number of new drawings. We found worsened verbal span, while phonemic verbal fluency remained unchanged. Interestingly, we observed a significant tendency to use the left part of each box in the m-FPT correlated with TAS-20 and with the subscales that assess difficulty in describing and identifying feelings. Although our data were collected from a small sample, they evidence that the restriction of behaviors determines a leftward bias, suggesting a greater activation of the right hemisphere, intrinsically connected with the processing of non-verbal information and with the need to manage an emotional situation.
The Role of Language Proficiency in False Memory: A Mini Review
Authors: Suarez Mar , Beato Maria Soledad
Published: April 2021

Memory errors and, specifically, false memories in the Deese/Roediger–McDermott paradigm have been extensively studied in the past decades. Most studies have investigated false memory in monolinguals’ native or first language (L1), but interest has also grown in examining false memories in participants’ second language (L2) with different proficiency levels. The main purpose of this manuscript is to review the current state of knowledge on the role of language proficiency on false memories when participants encode and retrieve information in the same language. To do so, a systematic literature search was conducted, and the available studies were reviewed. These studies differed in, for example, age, language proficiency, or material characteristics, including both high and low associative strength lists, and they reported different results. In this review, we attempted to make sense of the apparently contradictory results by carefully identifying participants’ language dominance and L2 proficiency. Specifically, the results indicated that, first, people are more prone to produce false memories in their dominant than in their non-dominant language. This result generalizes to lists with high and low associative strength, as well as to participants of different ages. Second, false memories do not differ between two languages when speakers are equally proficient in both languages. Finally, highly proficient L2 speakers produce more false memories in their L2 than speakers with lower L2 proficiency. The results of this review will be considered in the light of the theoretical frameworks of false memories and bilingual language processing.
Empathy and Theory of Mind in Multiple Sclerosis: A Meta-Analysis
Authors: Lin XiaoGuang , Zhang XueLing , Liu QinQin , Zhao PanWen , Zhong JianGuo , Pan PingLei , Wang GenDi , Yi ZhongQuan
Published: April 2021

Multiple sclerosis (MS) is an immune-mediated demyelinating disease of the central nervous system. Studies have shown that MS disrupts several social cognitive abilities [including empathy and theory of mind (ToM)]. Overall ToM deficits in MS are well documented, but how the specific ToM subcomponents and empathic capacity are affected remains unclear. For this meta-analysis, we searched PubMed, Web of Science, and Embase from inception to July 2020. Effect sizes were calculated using Hedges g with a random-effects model. Thirty-three studies were included. Relative to healthy controls (HCs), patients with MS were moderately impaired in overall empathy (g = −0.67), overall ToM (g = −74), cognitive ToM (g = −0.72), and the overlapping domains of cognitive empathy/affective ToM (g = −0.79); no group differences were identified for affective empathy (g = −0.19). Compared with HCs, patients with relapsing-remitting MS (RRMS) and progressive MS were impaired in overall empathy, overall ToM, cognitive ToM, and cognitive empathy/affective ToM, without significant RRMS–progressive MS differences in impairment degree. We conducted the first meta-analytic review investigating the empathy and ToM functioning patterns in patients with MS and examined the overlapping and distinct subcomponents of these constructs. The findings suggest differential impairment of the core aspects of social cognitive processing in patients with MS, which may importantly inform the development of structured social cognitive MS interventions.
NMDAR Neurotransmission Needed for Persistent Neuronal Firing: Potential Roles in Mental Disorders
Authors: Yang Shengtao , Seo Hyojung , Wang Min , Arnsten Amy F. T.
Published: April 2021

The dorsolateral prefrontal cortex (dlPFC) generates the mental representations that are the foundation of abstract thought, and provides top-down regulation of emotion through projections to the medial PFC and cingulate cortices. Physiological recordings from dlPFC Delay cells have shown that the generation of mental representations during working memory relies on NMDAR neurotransmission, with surprisingly little contribution from AMPAR. Systemic administration of low “antidepressant” doses of the NMDAR antagonist, ketamine, erodes these representations and reduces dlPFC Delay cell firing. In contrast to the dlPFC, V1 neuronal firing to visual stimuli depends on AMPAR, with much less contribution from NMDAR. Similarly, neurons in the dlPFC that respond to sensory events (cue cells, response feedback cells) rely on AMPAR, and systemic ketamine increases their firing. Insults to NMDAR transmission, and the impaired ability for dlPFC to generate mental representations, may contribute to cognitive deficits in schizophrenia, e.g., from genetic insults that weaken NMDAR transmission, or from blockade of NMDAR by kynurenic acid. Elevated levels of kynurenic acid in dlPFC may also contribute to cognitive deficits in other disorders with pronounced neuroinflammation (e.g., Alzheimer's disease), or peripheral infections where kynurenine can enter brain (e.g., delirium from sepsis, “brain fog” in COVID19). Much less is known about NMDAR actions in the primate cingulate cortices. However, NMDAR neurotransmission appears to process the affective and visceral responses to pain and other aversive experiences mediated by the cingulate cortices, which may contribute to sustained alterations in mood state. We hypothesize that the very rapid, antidepressant effects of intranasal ketamine may involve the disruption of NMDAR-generated aversive mood states by the anterior and subgenual cingulate cortices, providing a “foot in the door” to allow the subsequent return of top-down regulation by higher PFC areas. Thus, the detrimental vs. therapeutic effects of NMDAR blockade may be circuit dependent.
Health Literacy Mediates the Association Between Socioeconomic Status and Productive Aging Among Elderly Chinese Adults in a Newly Urbanized Community
Authors: Ma Tianpei , Meng Hongdao , Ye Zhiqiu , Jia Chaoyong , Sun Min , Liu Danping
Published: April 2021

Productive aging, or older adults engaging in paid or unpaid activities that produce socially valued goods or services, has been suggested to have the beneficial impact on older adults' health and well-being. We performed a cross-sectional study to examine the influence of health literacy on the relationship between socioeconomic status (SES) and productive aging among older Chinese adults in a newly urbanized community. Data was collected from 995 older adults from a newly urbanized community between June and August 2013 in Chengdu, China. We used structural equation modeling (SEM) to test the hypothesized relationship among SES, health literacy and productive aging. Results showed that education attainment and income had a direct positive effect on health literacy (β = 0.47and β = 0.15, respectively). Education had a partial indirect effect on productive aging through health literacy (β = 0.27). And health literacy was an important factor in improving the productive aging of the elderly. Interventions targeting health education and health promotion should be taken to improve health literacy of older adults under the background of urbanization, especially for those with lower SES.
Can Robots Earn Our Trust the Same Way Humans Do? A Systematic Exploration of Competence, Warmth, and Anthropomorphism as Determinants of Trust Development in HRI
Authors: Christoforakos Lara , Gallucci Alessio , Surmava-Große Tinatini , Ullrich Daniel , Diefenbach Sarah
Published: April 2021

Robots increasingly act as our social counterparts in domains such as healthcare and retail. For these human-robot interactions (HRI) to be effective, a question arises on whether we trust robots the same way we trust humans. We investigated whether the determinants competence and warmth, known to influence interpersonal trust development, influence trust development in HRI, and what role anthropomorphism plays in this interrelation. In two online studies with 2 × 2 between-subjects design, we investigated the role of robot competence (Study 1) and robot warmth (Study 2) in trust development in HRI. Each study explored the role of robot anthropomorphism in the respective interrelation. Videos showing an HRI were used for manipulations of robot competence (through varying gameplay competence) and robot anthropomorphism (through verbal and non-verbal design cues and the robot's presentation within the study introduction) in Study 1 (n= 155) as well as robot warmth (through varying compatibility of intentions with the human player) and robot anthropomorphism (same as Study 1) in Study 2 (n= 157). Results show a positive effect of robot competence (Study 1) and robot warmth (Study 2) on trust development in robots regarding anticipated trust and attributed trustworthiness. Subjective perceptions of competence (Study 1) and warmth (Study 2) mediated the interrelations in question. Considering applied manipulations, robot anthropomorphism neither moderated interrelations of robot competence and trust (Study 1) nor robot warmth and trust (Study 2). Considering subjective perceptions, perceived anthropomorphism moderated the effect of perceived competence (Study 1) and perceived warmth (Study 2) on trust on an attributional level. Overall results support the importance of robot competence and warmth for trust development in HRI and imply transferability regarding determinants of trust development in interpersonal interaction to HRI. Results indicate a possible role of perceived anthropomorphism in these interrelations and support a combined consideration of these variables in future studies. Insights deepen the understanding of key variables and their interaction in trust dynamics in HRI and suggest possibly relevant design factors to enable appropriate trust levels and a resulting desirable HRI. Methodological and conceptual limitations underline benefits of a rather robot-specific approach for future research.
Modeling of Thermal-Hydrological-Chemical (THC) Processes During Waste Rock Weathering Under Permafrost Conditions
Authors: Yi Xueying , Su Danyang , Seigneur Nicolas , Mayer Klaus Ulrich
Published: April 2021

The oxidation of sulfide minerals such as pyrite present in waste rock results in elevated sulfate, enhanced metal loadings and in many cases low pH conditions. Recently, many mines have opened in remote areas, including regions subject to permafrost conditions. In these regions, freeze-thaw cycles and the possible development of permafrost in mine waste add to the complexity of weathering processes, drainage volumes and mass loadings. To assess weathering in these waste rock piles, the reactive transport code MIN3P-HPC has been enhanced by implementing constitutive relationships related to freeze-thaw cycles that control flow patterns, solute transport, generation and transport of heat, as well as geochemical reactions and their rates. Simulations of a hypothetical pyrite-rich waste rock pile placed onto natural permafrost were conducted under reference climate conditions. Additionally, the effect of a warming climate was also studied through a sensitivity analysis. The simulation results indicate a potentially strong coupled effect of sulfide mineral weathering rates and a warming climate on the evolution and persistence of permafrost within waste rock piles and the release of acidic drainage. For relatively low sulfide mineral oxidation rates, the simulations indicate that permafrost can develop within waste rock piles, even under warming climate conditions. However, the results for low reactivity also show that mass loadings can increase by >50% in response to a slight warming of climate (3°C), relative to reference climate conditions. For the chosen reference reaction rates, permafrost develops under reference climate conditions in the simulated waste rock pile; however, permafrost cannot be maintained for a marginally warmer climate, leading to internal heating of the pile and substantially increased production of acidic drainage (>550%). For high reaction rates, the simulations suggest that internal heating takes place irrespective of climate conditions. Evaluation of thermal covers indicates that significant reductions of mass loadings can be achieved for piles with low and reference reactivity (91–99% in comparison to uncovered piles), but also suggest that thermal covers can be ineffective for piles with high sulfide content and reactivity. Together, these simulations provide insights into the complex interactions controlling waste rock weathering in cold-region climates.
Hoof Pressure Distribution Pattern of Blue Sheep During Walking on Different Slopes: A Subject-Specific Analysis
Authors: Liu Xiangyu , Kui Hailin , Qian Zhihui , Ren Lei
Published: April 2021

The purpose of this study was to quantitatively assess the vertical force distribution (VFD) of subject-specific healthy blue sheep while walking on different slopes using a pressure-sensing walkway. The blue sheep was trained to walk over the pressure-sensing walkway by choosing a comfortable walking speed, and the slope angle increased from 0° to 25°. The sheep's hooves were divided into four quadrants, namely, the cranio-lateral, cranio-medial, caudo-lateral, and caudo-medial quadrants, to investigate the VFD of the peak vertical force (PVF), vertical impulse (VI) and occurrence time of the PVF during the stance phase (TPVF). This study demonstrates that the main stressed quadrant of the front hoof changes from the caudo-medial quadrant to the cranio-medial quadrant with increasing slope. The main stressed quadrant of the rear hoof is the cranio-medial quadrant and does not change with the increasing slope. For all the slopes, the vertical force shifted from the lateral quadrant to the medial quadrant and from the caudal quadrant to the cranial quadrant. All the results obtained in the study suggest the feasibility of detecting gait changes in blue sheep, which has potential for the diagnosis of lower limb musculoskeletal diseases in quadrupeds.
Comparison of the Ability of High and Low Virulence Strains of Non-cytopathic Bovine Viral Diarrhea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium
Authors: Wang Kai , Thomas Carole , Zhang Shujun , Wathes D. Claire , Cheng Zhangrui
Published: April 2021

Bovine Viral Diarrhea virus (BVDV) is a pestivirus with a single-stranded, positive sense RNA genome. It is endemic in many cattle populations, causing major economic losses in part due to reduced fertility. BVDV exhibits great genetic diversity and is classified as type 1 or 2 (BVDV-1, BVDV-2) with either non-cytopathogenic (ncp) or cytopathogenic (cp) biotypes. Differing strains of ncpBVDV differ in virulence, affecting clinical outcome. BVDV replicates in the reproductive tract, affecting host immunity and embryo survival. This study used an in vitro model of primary bovine endometrial cell cultures to compare the effects of two BVDV ncp type 1a strains of differing virulence (termed HO and KY) on endometrial transcription of candidate interferon stimulated genes (ISG) using qPCR. Half the cultures were stimulated with interferon tau (IFNT, the conceptus produced pregnancy recognition factor) in the presence or absence of viral infection. Cultures were replicated on cells from 10 BVDV-free cows. IFNT treatment stimulated transcription of 10 candidate ISGs, whereas both ncpBVDV-1 strains alone inhibited transcription of 8/10 ISGs. In combined BVDV-1+IFNT cultures, the stimulatory effect of IFNT on expression of GBP4, ISG15, HERC5, RSAD2, IFIH1, IFIT3, and MX1 was significantly inhibited by HO, but only ISG15, RSAD2, IFI27, and IFIT3 were decreased by KY. Inhibition by HO was generally greater. The IFNT-induced expression of TRIM56 was, however, increased by HO. These data show that HO, the more virulent ncpBVDV-1 strain, has a greater capacity to inhibit key antiviral pathways. These differences need confirmation at the protein level but may influence immune tolerance of the host. They could also reduce fertility by increasing uterine susceptibility to bacterial infection and disrupting IFNT-mediated pregnancy recognition.
The Dessau Grassland Experiment—Impact of Fertilization on Forage Quality and Species Assembly in a Species-Rich Alluvial Meadow
Authors: Dullau Sandra , Rydgren Knut , Kirmer Anita , Jäger Urs Georg , Meyer Maren Helen , Tischew Sabine
Published: April 2021

Since alluvial meadows of river valleys of the Cnidion dubii are protected by the EU Habitats Directive, reconciling farmers’ demands for forage quality with the objective of maintaining them in good conservation status is an important issue in grassland research. In a long-term experiment from 2010 to 2018, we investigated the impact of fertilizing on forage quality and species assembly on a species-rich and twice-mown alluvial grassland in the Dessau Elbe floodplain (Germany). The experiment was composed of an unfertilized control, PK, N60, N60PK and N120PK applications. A significant improvement in forage quality was achieved by nitrogen fertilization only for crude protein, with higher feeding requirements for sheep met only in individual years. The legume cycle was inhibited by the application of nitrogen and high grass cover was maintained, but not increased, at the highest nitrogen application after an exceptional summer flood. The target forbs persisted in numbers over the study period in all treatments. For cover, the low-competitive target forbs responded neutrally to nitrogen fertilization, whereas detrimental effects were demonstrated for the competitive ones. Thus, we recommend not applying more than 60 kg year−1 of nitrogen and only in combination with phosphorus and potassium.
CHAP: Cotton-Harvesting Autonomous Platform
Authors: Maja Joe Mari , Polak Matthew , Burce Marlowe Edgar , Barnes Edward
Published: April 2021

The US cotton industry provided over 190,000 jobs and more than $28 billion total economic contributions to the United States in 2012. The US is the third-largest cotton-producing country in the world, following India and China. US cotton producers have been able to stay competitive with countries like India and China by adopting the latest technologies. Despite the success of technology adoption, there are still many challenges, e.g., increased pest resistance, mainly glyphosate resistant weeds, and early indications of bollworm resistance to Bt cotton (genetically modified cotton that contains genes for an insecticide). Commercial small unmanned ground vehicle (UGV) or mobile ground robots with navigation-sensing modality provide a platform to increase farm management efficiency. The platform can be retrofitted with different implements that perform a specific task, e.g., spraying, scouting (having multiple sensors), phenotyping, harvesting, etc. This paper presents a proof-of-concept cotton harvesting robot. The robot was retrofitted with a vacuum-type system with a small storage bin. A single harvesting nozzle was used and positioned based on where most cotton bolls were expected. The idea is to create a simplified system where cotton bolls′ localization was undertaken as a posteriori information, rather than a real-time cotton boll detection. Performance evaluation for the cotton harvesting was performed in terms of how effective the harvester suctions the cotton bolls and the effective distance of the suction to the cotton bolls. Preliminary results on field test showed an average of 57.4% success rate in harvesting locks about 12 mm from the harvester nozzle. The results showed that 40.7% was harvested on Row A while 74.1% in Row B for the two-row test. Although both results were promising, further improvements are needed in the design of the harvesting module to make it suitable for farm applications.
The Most Important Fungal Diseases of Cereals—Problems and Possible Solutions
Authors: Różewicz Marcin , Wyzińska Marta , Grabiński Jerzy
Published: April 2021

The level of cereal yields and the quality of these yields depend, to a large extent, on a crop management system, the genetic potential of a given cultivar, but also on factors that may cause damage to plants or a reduction in yield. Such factors include fungal diseases of cereals, which may cause a reduction in yield by 15–20%, and in extreme cases even by 60%. The main factors determining the occurrence of these pathogens are the weather conditions during the growing season of plants, crop rotation, the previous crop, the soil tillage system, and nitrogen fertilisation. Fungal diseases of cereals limit plant growth and development, as well as reduce grain yield and quality. This paper reviews the literature on fungal diseases of cereals.
Sowing Date and Genotype Influence on Yield and Quality of Dual-Purpose Barley in a Salt-Affected Arid Region
Authors: Moustafa Ehab S. A. , El-Sobky El-Sayed E. A. , Farag Hossam I. A. , Yasin Mohamed A. T. , Attia Ahmed , Rady Mohamed O. A. , Awad Mohamed F. , Mansour Elsayed
Published: April 2021

Dual-purpose barley is an alternative approach to producing high-quality forage yield plus an acceptable grain yield in marginal environments of arid regions that are characterized by lack of forage. Field experiment was performed in two consecutive growing seasons at an arid region affected by salinity in irrigation water and soil at Western Sinai Peninsula in Egypt. The study aimed to optimize sowing date and screen salt-tolerant barley genotypes that perform better in terms of forage yield and quality as well as grain and biomass yield production in salt-affected environment. Sowing dates, genotypes, and their interaction significantly impacted most of the studied variables such as forage yield, crude protein yield, and grain and biomass yields. The early sowing in late October yielded higher than intermediate sowing in mid-November and late sowing in early December. Some of the tested genotypes performed better than others as indicated by about 50% higher forage yield, 6% crude protein content, 39% grain and 21% biological yields (total aboveground dry matter), suggesting higher adaptation capacity. Interestingly, grain and biological yields did not differ significantly between dual-purpose approach and grain-only pattern. In conclusion, dual-purpose barley was found favorable for producing grain and forage production in similar environments under early sowing date.
Precrop Effect of Red Clover (Trifolium pratense L.) and Alfalfa (Medicago sativa L.) on the Population Development of the Northern Root-Knot Nematode Meloidogyne hapla Chitwood, 1949 and on Succeeding Crops—A Pot Study
Authors: Dobosz Renata , Krawczyk Roman
Published: April 2021

The northern root-knot nematode, Meloidogyne hapla, is a major pest of many crop species. The objective of the study was to determine how M. hapla population dynamics is affected by two precrops, i.e., Trifolium pratense and Medicago sativa, in three crop durations: one, two and three years of continuous cultivation. Moreover, we set ourselves the task of evaluating the effect of the legume precrop soil on the growth of the succeeding tomato plant (Solanum lycopersicum) and on the nematode population. The experiment was performed outdoors in pots with naturally infected soil. Both precrop species investigated were found to modify the J2 nematode population density in the soil. The galls and nematode females with egg masses were observed on the roots of both studied plant species at the end of each growing season. They appeared to be more abundant on the red clover roots than on those of the alfalfa. The obtained data indicate that the spring soil sampling is more appropriate for the estimation of the M. hapla population density in the red clover precrop soil. The legume precrop soil had a limiting effect on tomato growth and fruit yield. The nematode population negatively influenced tomato growth. The experiment revealed that tomato plants could be planted in alfalfa precrop soil following at least three years of continuous alfalfa cultivation. The same cannot be said of the cultivation of red clover as a precrop for tomatoes.
Influence of Tillage and Crop Rotations in Organic and Conventional Farming Systems on Soil Organic Matter, Bulk Density and Enzymatic Activities in a Short-Term Field Experiment
Authors: Pittarello Marco , Dal Ferro Nicola , Chiarini Francesca , Morari Francesco , Carletti Paolo
Published: April 2021

Intensive agricultural practices are leading to loss of soil fertility and overexploitation of natural resources which cause nutrients imbalance and further impair ecosystem services. Organic farming (OF), also coupled with minimum tillage and crop rotations, represents one of the strategies to limit this process and maintain soil functions. In a two-year field trial, organic farming practices, including a set of fertilizations combined with crop rotations and association with nitrogen fixing cover crops, were compared. The aim of this research was to assess in the short-term the effects on soil organic carbon, aggregate stability, and soil enzymes activities of using a combination of promising management practices in the delta region of the Po river. Results did not show improvements in organic carbon content and soil aggregate stability. Conversely, enzymatic activities were always significantly higher in OF treatments than the conventional one. Crop rotation and associated legumes were effective in enhancing β-glucosidase and P fixation through phosphatases activities. The present work suggests that an effective choice of crop species coupled with legumes can enhance biological activity re-starting main mechanisms of microbial development even without a contemporary increase of organic matter.
Seed Priming and Coating by Nano-Scale Zinc Oxide Particles Improved Vegetative Growth, Yield and Quality of Fodder Maize (Zea mays)
Authors: Tondey Manisha , Kalia Anu , Singh Alla , Dheri Gurmeet Singh , Taggar Monica Sachdeva , Nepovimova Eugenie , Krejcar Ondrej , Kuca Kamil
Published: April 2021

Nano-fertilizers of essential plant nutrients, including micronutrients, have the potential to improve nutrient use efficiency and productivity of field crops in deficient soils. The present study reports the comparative influence of zinc oxide nanoparticles (ZnONPs) and bulk Zn salt (ZnSO4) on the growth, yield, and quality of fodder maize (Zea mays) (var. J-1006) cultivated under field conditions in the year 2019. Three levels (0, 20, and 40 mg L−1) of Zn fertilizers were used for seed priming and coating in triplicate following the randomized complete block design model. An increase in vegetative and yield parameters (number of plants, plant height, stover yield, plant biomass), acid detergent fiber (ADF%), and hemicellulose contents and shoot zinc (Zn) content on treatment of seeds with ZnONPs (20 mg L−1) concentration as compared to bulk ZnSO4 and control treatments was observed. The application of ZnONPs (40 mg L−1) significantly enhanced the total chlorophyll content, available soil nitrogen and phosphorus, neutral detergent fiber (NDF%), and cellulose contents and improved the total soil microbial counts and soil enzyme activities (dehydrogenase, acid and alkaline phosphatase enzyme activities), whereas a significant increase in available soil potassium and zinc contents was recorded under ZnONPs (20 mg L−1) treatments. These findings suggest an encouraging effect on the growth and yield attributing characteristics of fodder maize after ZnONPs seed coating at low concentration. Furthermore, ZnONPs seed coating can also be considered an effective tool for the delivery of Zn micronutrient to fodder maize crop.