bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Transcriptomic Profiling of Brain and Liver in Phoenix Barbs (Spinibarbus denticulatus denticulatus) with Differential Growth Rates
Authors: Xie Xi , Zhuang Jiamiao , Liao Xianping , Xu Zhengsheng , Liang Wenlang , Su Yilin , Lin Li , Xie Jungang , Lin Weiqiang
Published: October 2024

Phoenix barb (Spinibarbus denticulatus denticulatus) is a notable fish species in South China and is valued for its ecological and economic importance. To elucidate the molecular basis underlying its growth, we collected transcriptome profiles from the brains and livers of individual fish with different growth rates and compared differentially expressed genes (DEGs) at 3 and 9 months after hatching (MAH). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the pathways associated with growth were predominantly enriched in fatty acid biosynthesis, AMPK signaling, PI3K-Akt signaling, estrogen signaling, and protein metabolism. Notably, a greater number of DEGs from the fast-growing group were associated with these pathways at the early growth stage (3 MAH) than at the later stage (9 MAH). Real-time quantitative PCR results further validated that the genes involved in these pathways exhibited higher expression levels in fast-growing samples. This study enhances our understanding of the genetic mechanisms underlying growth rate differences and provides valuable genetic resources for future growth-related molecular breeding programs of phoenix barbs.
Okra (Abelmoschus esculentus L.) Flour Integration in Wheat-Based Sourdough: Effect on Nutritional and Technological Quality of Bread
Authors: Valerio Francesca , Di Biase Mariaelena , Cifarelli Valentina , Lonigro Stella Lisa , Maalej Amina , Plazzotta Stella , Manzocco Lara , Calligaris Sonia , Maalej Hana
Published: October 2024

The aim of this study was to develop an innovative sourdough using dehydrated okra (Abelmoschus esculentus L.) pod flour and to use it in the production of bread. Three different flours (sun-dried S, freeze-dried F, oven-dried O) were individually mixed at 9% with wheat flour (Dough Yield 300) and fermented (N0: 8.0 log10 CFU/g) for 14 h, using Lactiplantibacillus plantarum ITM21B, Weissella cibaria C43-11 or Leuconostoc mesenteroides C43-2M. The results showed that after fermentation, the content of organic acids (lactic, acetic and propionic), exopolysaccharides (EPS), l-glutamic acid and total free amino acids (TFAA) increased and the high molecular weight proteins were converted into smaller proteins. Sourdough based on Leuc. mesenteroides and O flour (O_LeuMes) was selected to evaluate its applicability in bread making. It was included in the yeast-leavened bread formulation at 20 or 40% (0.6% and 1.21% w/w O flour replacement). The results showed that fermentation limited the negative effects of unfermented O flour on bread quality attributes, mainly the specific volume and firmness. Bread with O_LeuMes at 40% was improved in TFAA, EPS and l-glutamic acid content and showed a higher specific volume and lower moisture and firmness compared to bread with the unfermented O flour.
Qualitative and Quantitative Sex-Related Differences in the Perception of Single Molecules from Coffee Headspace
Authors: Sollai Giorgia , Solari Paolo , Crnjar Roberto
Published: October 2024

One of the still-debated topics regarding the olfactory function concerns the presence or absence of sex-related differences in individuals. In this study, we checked for a relationship between the olfactory function of females and males and their ability to perceive single molecules, and researched how this can influence the intensity with which the complex odor formed by a set of single molecules is perceived. First, females and males were classified as normosmic or hyposmic based on the TDI olfactory score obtained using the Sniffin’ Sticks test. Subsequently, the headspace of roasted coffee beans, as a complex olfactory stimulus, was broken down into single molecules by means of a chromatographic column; these were simultaneously conveyed to a mass spectrometer (for their subsequent classification) and to the human nose, which acts as a chemical sensor by means of an olfactometer port. The results obtained with this gas chromatography–olfactometry approach show both qualitative and quantitative differences between females and males, with females performing better than males. In addition, the odor intensity reported by females when sniffing pen #10, containing coffee aroma, is significantly higher than that reported by males. In conclusion, these data highlight that the human ability to perceive both single compounds and complex odors is strongly conditioned, not only by the olfactory function of individuals, but also by their sex.
Quantitative Analysis of High-Price Rice Adulteration Based on Near-Infrared Spectroscopy Combined with Chemometrics
Authors: Chen Mengting , Song Jiahui , He Haiyan , Yu Yue , Wang Ruoni , Huang Yue , Li Zhanming
Published: October 2024

Near-infrared spectroscopy (NIRS) holds significant promise in detecting food adulteration due to its non-destructive, simple, and user-friendly properties. This study employed NIRS in conjunction with chemometrics to estimate the content of low-price rice flours (Nanjing, Songjing, Jiangxi silk, Yunhui) blended with high-price rice (Wuchang and Thai fragrant). Partial least squares regression (PLSR), support vector regression (SVR), and back-propagation neural network (BPNN) models were deployed to analyze the spectral data of adulterated samples and assess the degree of contamination. Various preprocessing techniques, parameter optimization strategies, and wavelength selection methods were employed to enhance model accuracy. With correlation coefficients exceeding 87%, the BPNN models exhibited high accuracy in estimating adulteration levels in high-price rice. The SPXY-SG-BPNN, SPXY-MMN-BPNN, KS-SNV-BPNN, and SPXY-SG-BPNN models showcased exceptional performance in discerning mixed Wuchang japonica, Thai fragrant indica, and Thai fragrant Yunhui rice. As shown above, NIRS demonstrated its potential as a rapid, non-destructive method for detecting low-price rice in premium rice blends. Future studies should be performed to concentrate on enhancing the models’ versatility and practical applicability.
Characteristics and Bioactivities of Protein Hydrolysate from Cricket (Acheta domesticus) Powder Defatted Using Ethanol with Aid of Vacuum Impregnation
Authors: Chotphruethipong Lalita , Senphan Theeraphol , Sigh Avtar , Hutamekalin Pilaiwanwadee , Nuthong Pornpot , Benjakul Soottawat
Published: October 2024

Cricket is a potential proteinaceous source used for protein hydrolysate (PH) preparation, having several biological activities. Nevertheless, cricket has high lipid contents, which are susceptible to oxidation during PH preparation. Thus, ethanol was used together with vacuum impregnation (VI) to enhance defatting efficacy before PH preparation. Also, bioavailability of the digest of PH after gastrointestinal tract (GIT) digestion via the Caco-2 monolayer was assessed. Cricket powder was defatted using ethanol for 1–4 h. Lipid contents were decreased with enhancing time until 2 h. Additionally, the defatting efficacy was augmented when ethanol combined with VI at 4 cycles for 2 h (VI-E-2) was implemented. Lowered mono- and polyunsaturated fatty acid contents were also observed in the VI-E-2 sample. The VI-E-2 sample was used to prepare PH using Alcalase and Flavourzyme (0.2–0.4 units/g dry sample). PH prepared by Alcalase hydrolysis at 0.2 units/g dry sample (A-0.2) showed the higher ABTS radical-scavenging activity and FRAP, compared to that prepared by Flavourzyme hydrolysis (p < 0.05). Thus, the A-0.2 sample was selected for digestion via the GIT system. The obtained digest (500–1000 μg/mL) had bioavailability of peptides, depending on the levels used. Therefore, PH from defatted cricket powder could be a promising ingredient for food applications.
Antibacterial Effects of Thermosonication Technology on Salmonella typhimurium Strains Identified from Swine Food Chain: An In Vitro Study
Authors: Pennisi Luca , Ferri Gianluigi , Lauteri Carlotta , Di Clerico Daniele , Vergara Alberto
Published: October 2024

Among innovative food technologies, ultrasounds have demonstrated physical damages (provided by frequency and intensity factors) on bacterial structures while determining the microbiological stabilization of many foodstuffs. This study tested the efficacy of the thermosonication process on 16 Salmonella typhimurium strains belonging to the academic biobank (isolated from swine slaughterhouses). All strains were exposed to focused ultrasounds, generated by the Waveco® system (Milan, Italy), with the following settings: 40 KHz coupled with 80 W at different 5 min intervals starting from 5 to 15 ones, and focusing on two different temperatures: 40 °C and 50 °C. After each treatment, all strains were directly plated onto count agars immediately (t0) and after 24 h (t24) of storage at refrigerated temperature. The results showed bacterial reductions by prolonging the sonication treatments until 15 min (i.e., 50 °C for 15 min reduced of 2.16 log CFU/gr the initial loads). In the present in vitro study, the most considerable decrease was observed after 24 h. It meant that Salmonella strains were lethally damaged at the wall level, confirming the ultrasound bactericidal effect on loads. The present in vitro scientific investigation demonstrates the practical bactericidal effects of thermosonication, highlighting promising applications at the industry level for food microbial stabilization and shelf-life prolongation.
Fractional Consistent Riccati Expansion Method and Soliton-Cnoidal Solutions for the Time-Fractional Extended Shallow Water Wave Equation in (2 + 1)-Dimension
Authors: Zhang Lihua , Shen Bo , Jia Meizhi , Wang Zhenli , Wang Gangwei
Published: October 2024

In this work, a fractional consistent Riccati expansion (FCRE) method is proposed to seek soliton and soliton-cnoidal solutions for fractional nonlinear evolutional equations. The method is illustrated by the time-fractional extended shallow water wave equation in the (2 + 1)-dimension, which includes a lot of KdV-type equations as particular cases, such as the KdV equation, potential KdV equation, Boiti–Leon–Manna–Pempinelli (BLMP) equation, and so on. A rich variety of exact solutions, including soliton solutions, soliton-cnoidal solutions, and three-wave interaction solutions, have been obtained. Comparing with the fractional sub-equation method, G′/G-expansion method, and exp-function method, the proposed method gives new results. The method presented here can also be applied to other fractional nonlinear evolutional equations.
Fractal Dimension Analysis of the Tumor Microenvironment in Cutaneous Squamous Cell Carcinoma: Insights into Angiogenesis and Immune Cell Infiltration
Authors: Buruiană Alexandra , Șerbănescu Mircea-Sebastian , Pop Bogdan , Gheban Bogdan-Alexandru , Gheban-Roșca Ioana-Andreea , Hendea Raluca Maria , Georgiu Carmen , Crișan Doinița , Crișan Maria
Published: October 2024

The global incidence of cutaneous squamous cell carcinoma (cSCC), a prevalent and aggressive skin cancer, has risen significantly, posing a substantial public health challenge. This study investigates the tumor microenvironment (TME) of cSCC by focusing on the spatial distribution patterns of immune and vascular markers (CD31, CD20, CD4, and CD8) using fractal dimension (FD) analysis. Our analysis encompassed 141 cases, including 100 invasive cSCCs and 41 specimens with pre-invasive lesions exclusively, and the rest were peripheral pre-invasive lesions from the invasive cSCC class. The FD values for each marker were computed and compared between pre-invasive and invasive lesion classes. The results revealed significant differences in FD values between the two classes for CD20 and CD31 markers, suggesting distinct alterations in B cell distribution and angiogenic activity during cSCC progression. However, CD4 and CD8 markers did not exhibit significant changes individually. Still, the CD4/CD8 ratio showed a significant difference, suggesting a potential shift in the balance between T helper and cytotoxic T cell responses, impacting the immune landscape as lesions progressed from pre-invasive to invasive stages. These findings underscore the complexity and heterogeneity of the TME in cSCC and highlight the potential of FD analysis as a quantitative tool for characterizing tumor progression. Further research is needed to elucidate the implications of these differences in the clinical management of cSCC.
Metagenomic Sequencing Elucidated the Microbial Diversity of Rearing Water Environments for Sichuan Taimen (Hucho bleekeri)
Authors: Wei Qinyao , Song Zhaobin , Chen Yeyu , Yang Huanchao , Chen Yanling , Liu Zhao , Yu Yi , Tu Quanyu , Du Jun , Li Hua
Published: October 2024

Background: Sichuan taimen (Hucho bleekeri) is a fish species endemic to China’s upper Yangtze River drainage and has significant value as an aquatic resource. It was listed as a first-class state-protected wild animal by the Chinese government due to its very limited distribution and wild population at present. Methods: To elucidate the diversity of microorganisms in rearing water environments for H. bleekeri, metagenomic sequencing was applied to water samples from the Maerkang and Jiguanshan fish farms, where H. bleekeri were reared. Results: The results revealed that Pseudomonadota was the dominant phylum in the microbial communities of the water samples. Among the shared bacterial groups, Cyanobacteriota, Actinomycetota, Planctomycetota, Nitrospirota, and Verrucomicrobiota were significantly enriched in the water environment of Jiguanshan (p < 0.01), while Bacteroidota was more enriched in that of Maerkang (p < 0.01). Additionally, the Shannon diversity and Simpson index of the microbial community in the water environment of Maerkang were lower than in that of Jiguanshan. Conclusions: The present study demonstrated the similarities and differences in the microbial compositions of rearing water environments for H. bleekeri, which are expected to benefit the artificial breeding of H. bleekeri in the future.
Simple Sequence Repeat Marker-Based Genetic Diversity and Chemical Composition Analysis of Ancient Camellia sinensis in Jiulong County, Sichuan Province, China
Authors: Huang Haitao , He Shuwen , Zheng Xuxia , Shi Daliang , Bai Peixian , Zhao Yun , Yu Jizhong , Niu Xiaojun
Published: October 2024

Background/Objectives: The ancient tea plant germplasm resources are rich in genetic diversity and provide an important basis for the genetic diversity in tea germplasm resources. To explore the genetic diversity of ancient tea plant germplasm resources in Jiulong County, Sichuan Province. Methods: 59 ancient tea tree germplasm resources were analyzed using simple sequence repeat (SSR) molecular markers and chemical composition analysis. Results: The results showed that a total of 83 alleles were amplified by 23 pairs of SSR primers, with an average observed allele number (Na) of 3.6 and an effective allele number (Ne) of 2.335. The average Shannon information index (I) and the polymorphic information content (PIC) of the primers were 0.896 and 0.446, respectively. The results of the UPGMA cluster analysis showed that 59 ancient tea tree samples could be classified into five different subgroups. Based on the results of chemical composition analysis, two specific tea germplasm resources with high amino acid content, 10 excellent germplasm resources with tea polyphenol content over 20% and some other tea germplasm resources were identified. Conclusions: This study reveals that Jiulong’s ancient tea tree germplasm exhibits significant genetic diversity and includes valuable tea tree planting resources. These findings provide a foundational framework for the conservation, detailed exploration and sustainable utilization of these resources.
Characterization of Nuclear Mitochondrial Insertions in Canine Genome Assemblies
Authors: Schall Peter Z. , Meadows Jennifer R. S. , Ramos-Almodovar Fabian , Kidd Jeffrey M.
Published: October 2024

Background: The presence of mitochondrial sequences in the nuclear genome (Numts) confounds analyses of mitochondrial sequence variation, and is a potential source of false positives in disease studies. To improve the analysis of mitochondrial variation in canines, we completed a systematic assessment of Numt content across genome assemblies, canine populations and the carnivore lineage. Results: Centering our analysis on the UU_Cfam_GSD_1.0/canFam4/Mischka assembly, a commonly used reference in dog genetic variation studies, we found a total of 321 Numts located throughout the nuclear genome and encompassing the entire sequence of the mitochondria. A comparison with 14 canine genome assemblies identified 63 Numts with presence–absence dimorphism among dogs, wolves, and a coyote. Furthermore, a subset of Numts were maintained across carnivore evolutionary time (arctic fox, polar bear, cat), with eight sequences likely more than 10 million years old, and shared with the domestic cat. On a population level, using structural variant data from the Dog10K Consortium for 1879 dogs and wolves, we identified 11 Numts that are absent in at least one sample, as well as 53 Numts that are absent from the Mischka assembly. Conclusions: We highlight scenarios where the presence of Numts is a potentially confounding factor and provide an annotation of these sequences in canine genome assemblies. This resource will aid the identification and interpretation of polymorphisms in both somatic and germline mitochondrial studies in canines.
Advancements in the Impact of Insect Gut Microbiota on Host Feeding Behaviors
Authors: Wang Yikang , Wang Liang , Li Di , Chen Zhenfu , Luo Yang , Zhou Juan , Luo Bo , Yan Rong , Liu Hui , Wang Lingjun
Published: October 2024

With the application and development of high-throughput sequencing technology, the structure and function of insect gut microbiota have been analysed, which lays a foundation for further exploring the intricate relationships between gut microbiota and host feeding behaviour. The microbial community in the insect gut, as an important ecological factor, affects the host’s food selection and nutritional metabolic processes through various mechanisms, which play a key role in population dynamics and ecosystems. The implications of these interactions are profound, affecting agricultural practices, biodiversity, and the broader environment, such as pollination and pest control. In-depth exploration of the molecular mechanism of the interaction between gut microbiota and hosts contributes to the grasp of insect biology and evolution and offers novel avenues for manipulating insect behaviour for practical applications in agriculture and environmental management. This paper focuses on the possible mechanisms of insect gut microbiota regulating host feeding behaviour. It inspires further research on the interaction between gut microbiota and insects affecting host behaviour.
Cross-Species Susceptibility of Emerging Variants of SARS-CoV-2 Spike
Authors: Li Meng , Lv Fei , Li Zihao , Zhao Chenyu , Wang Xiao , Zhu Pingfen , Zhou Xuming
Published: October 2024

Background: The continuous evolution of SARS-CoV-2 and the emergence of novel variants with numerous mutations have heightened concerns surrounding the possibility of cross-species transmission and the establishment of natural animal reservoirs for the virus, but the host range of emerging SARS-CoV-2 variants has not been fully explored yet. Methods: We employed an in vitro model comprising VSV∆G* pseudotyped viruses bearing SARS-CoV-2 spike proteins to explore the plausible host range of SARS-CoV-2 emerging variants. Results: The overall host tropism of emerging SARS-CoV-2 variants are consistent with that of the SARS-CoV-2 wuhan-hu-1 strain with minor difference. Pseudotyped viruses bearing spike protein from RaTG13 and RmYN02 can enter cell cultures from a broad range of mammalian species, revealing that mink and hamsters may act as potential intermediate hosts. We further investigated 95 potential site-specific mutations in the SARS-CoV-2 spike protein that could impact viral infectivity across different species. The results showed that 13 of these mutations notably increased the transduction rates by more than two-fold when compared to the wild-type spike protein. Further examination of these 13 mutations within cell cultures from 31 different species revealed heightened sensitivity in cells derived from palm civets, minks, and Chinese horseshoe bats to the VSV∆G*-SARS2-S mutants. Specific mutations, such as L24F, R158G, and L212I, were seen to significantly enhance the capacity for SARS-CoV-2 of cross-species transmission. Conclusions: This study offers critical insights for the ongoing surveillance and monitoring efforts of SARS-CoV-2 evolution, emphasizing the need for the vigilant monitoring of specific mutations in both human and animal populations.
Comment on Park et al. Lessons Learned from Translating Genome Sequencing to Clinical Routine: Understanding the Accuracy of a Diagnostic Pipeline. Genes 2024, 15, 136
Authors: Battke Florian , Schulze Martin , Schulte Björn , Biskup Saskia
Published: October 2024

In their article, “Lessons Learned from Translating Genome Sequencing to Clinical Routine: Understanding the Accuracy of a Diagnostic Pipeline”, Haack et al [...]
APOBR Is Downregulated in EBV+ Tonsils of Children with Obstructive Sleep-Disordered Breathing
Authors: Santos-Cortez Regie Lyn P. , Gomez Helen Z. , Elling Christina L. , Mayher Landen , Diala Obinna R. , Gardner Colin , Willford Kiera , Zamora Valerie C. , Agyepong Ashley , Lee Nam K. , Green Katherine K. , Darr Owen A. , Wine Todd M. , Francom Christian R. , Larson Eric D. , Gitomer Sarah A. , Schell Amy E. , Frank Daniel N. , Friedman Norman R. , Herrmann Brian W.
Published: October 2024

Background: Obstructive sleep-disordered breathing (oSDB) is a heterogeneous phenotype that is increasing in prevalence worldwide and has many potential comorbidities that could severely affect quality of life. There is a need to identify biomarkers for oSDB and its comorbidities to improve clinical management, particularly in children. Methods: We performed bulk mRNA-sequencing, differential expression analysis, and qPCR replication of selected differentially expressed genes (DEGs) using RNA samples extracted from tonsils of children with oSDB. Two variables were used as classifier, namely, detection of Epstein–Barr virus (EBV) in tonsils and need for continuous positive airway pressure (CPAP) treatment. Standard statistical tests were used to determine associations across clinical, EBV, and DEG variables. Results: Nineteen genes were dysregulated in tonsils that are EBV+ or from children needing CPAP. Of these genes, APOBR was downregulated in both EBV+ and CPAP+ tonsils, and this downregulation was replicated by qPCR in an independent set of pediatric samples. In the tonsils of adult patients with oSDB, APOBR was positively correlated with age, and potentially with diastolic blood pressure. Conclusions: Taken together, APOBR and DEGs in tonsillar tissues may be useful as potential biomarkers of oSDB severity and comorbidity across the lifespan, with APOBR levels being dependent on latent EBV infection.
Simulating Compaction and Cementation of Clay Grain Coated Sands in a Modern Marginal Marine Sedimentary System
Authors: Houghton James E. , Nichols Thomas E. , Worden Richard H.
Published: October 2024

Reservoir quality prediction in deeply buried reservoirs represents a complex challenge to geoscientists. In sandstones, reservoir quality is determined by the extent of compaction and cementation during burial. During compaction, porosity is lost through the rearrangement and fracture of rigid grains and the deformation of ductile grains. During cementation, porosity is predominantly lost through the growth of quartz cement, although carbonate and clay mineral growth can be locally important. The degree of quartz cementation is influenced by the surface area of quartz available for overgrowth nucleation and thermal history. Clay grain coats can significantly reduce the surface area of quartz available for overgrowth nucleation, preventing extensive cementation. Using a coupled-effect compaction and cementation model, we have forward-modelled porosity evolution of surface sediments from the modern Ravenglass Estuary under different maximum burial conditions, between 2000 and 5000 m depth, to aid the understanding of reservoir quality distribution in a marginal marine setting. Seven sand-dominated sub-depositional environments were subject to five burial models to assess porosity-preservation in sedimentary facies. Under relatively shallow burial conditions (<3000 m), modelled porosity is highest (34 to 36%) in medium to coarse-grained outer-estuary sediments due to moderate sorting and minimal fine-grained matrix material. Fine-grained tidal flat sediments (mixed flats) experience a higher degree of porosity loss due to elevated matrix volumes (20 to 31%). Sediments subjected to deep burial (>4000 m) experience a significant reduction in porosity due to extensive quartz cementation. Porosity is reduced to 1% in outer estuary sediments that lack grain-coating clays. However, in tidal flat sediments with continuous clay grain coats, porosity values of up to 30% are maintained due to quartz cement inhibition. The modelling approach powerfully emphasises the value of collecting quantitative data from modern analogue sedimentary environments to reveal how optimum reservoir quality is not always in the coarsest or cleanest clastic sediments.
Electromagnetic and Radon Earthquake Precursors
Authors: Nikolopoulos Dimitrios , Cantzos Demetrios , Alam Aftab , Dimopoulos Stavros , Petraki Ermioni
Published: October 2024

Earthquake forecasting is arguably one of the most challenging tasks in Earth sciences owing to the high complexity of the earthquake process. Over the past 40 years, there has been a plethora of work on finding credible, consistent and accurate earthquake precursors. This paper is a cumulative survey on earthquake precursor research, arranged into two broad categories: electromagnetic precursors and radon precursors. In the first category, methods related to measuring electromagnetic radiation in a wide frequency range, i.e., from a few Hz to several MHz, are presented. Precursors based on optical and radar imaging acquired by spaceborne sensors are also considered, in the broad sense, as electromagnetic. In the second category, concentration measurements of radon gas found in soil and air, or even in ground water after being dissolved, form the basis of radon activity precursors. Well-established mathematical techniques for analysing data derived from electromagnetic radiation and radon concentration measurements are also described with an emphasis on fractal methods. Finally, physical models of earthquake generation and propagation aiming at interpreting the foundation of the aforementioned seismic precursors, are investigated.
Gender-Specific Impacts of the COVID-19 Pandemic on Orthopedic and Traumatology Care: An Analysis of Hospital Admissions and Length of Stay
Authors: Bancsik Karoly , Daina Lucia Georgeta , Lorenzovici László , Rossu György , Bancsik Raluca , Ghitea Timea Claudia , Ilea Codrin Dan Nicolae , Daina Mădălina Diana
Published: October 2024

Background: Understanding gender disparities in healthcare outcomes is crucial, especially during crises like the COVID-19 pandemic. The absence of gender-specific data on hospital admissions and lengths of stay for orthopedic and traumatology patients limits the precision of current analyses, making conclusions speculative. Objective: This study aims to highlight the potential insights that could be gained from gender-disaggregated data, illustrating how a more precise gender-based analysis could reveal healthcare disparities in orthopedic and trauma care during the pandemic. Materials and Methods: A robust analysis would require gender-disaggregated data, including variables such as admission rates, lengths of stay, injury types, and access to care, along with factors like age and socioeconomic status. In the absence of such data, a hypothetical framework was developed based on known healthcare disparities, using general trends to project possible gender-specific impacts. Results: Without gender-disaggregated data, it remains challenging to identify specific gender differences in outcomes accurately. Hypothetical scenarios suggest that disparities in admission rates, hospital stay durations, and access to care could be influenced by factors such as increased caregiving responsibilities for women or differential access to healthcare based on socioeconomic status. Conclusions: To accurately assess gender disparities in orthopedic and trauma care during the pandemic, future studies must prioritize the collection and use of gender-disaggregated data. This approach is essential for drawing reliable conclusions and developing targeted interventions to address healthcare inequities effectively.
High-Sensitivity Cardiac Troponin [hs-cTn] as a Valuable Biomarker for Pulmonary Hypertension Risk Stratification: A Contemporary Review of the Literature
Authors: Ganipineni Vijay Durga Pradeep , Jitta Sahas Reddy , Gudiwada Mohan Chandra Vinay Bharadwaj , Jasti Jaswanth Rao , Janga Chaitra , Merugu Bhavyasri , Bandaru Revanth Reddy , Puli Srikanth , Venkata Vikramaditya Samala , Vasavada Advait , Desai Rupak
Published: October 2024

Background: Pulmonary hypertension (PH) can lead to cardiac failure, thereby significantly affecting life expectancy and quality of life. Due to inadequate disease surveillance and risk assessment, clinical challenges persist despite advances in diagnosis and treatment. We aimed to review the potential of high-sensitivity cardiac troponin (hs-cTn) as a biomarker for predicting outcomes in PH patients. Methods: A thorough examination of the PubMed and Google Scholar databases was conducted through March 2023. Studies involving adult PH patients and hsTn as a prognostic indicator of outcomes such as mortality, hospitalization, and disease progression were included, after screening their titles and abstracts. Two independent evaluators extracted data, with the quality assessed using the JBI critical appraisal tool. Results: This review uncovered eight studies that examined the prognostic value of hs-cTn in PH patients. Higher hs-cTn levels were associated with increased mortality and hospitalization rates, according to the studies. The severity of PH, cardiac dysfunction, right ventricular function, and systolic dysfunction were associated with hs-cTn. Multiple studies have demonstrated that hsTn has the potential to identify high-risk PH patients who could benefit from targeted therapies and increased clinical monitoring. Conclusions: This review suggests that hsTn may be a biomarker for PH risk stratification and prognosis. Across PH subtypes, elevated hsTn levels predict poor outcomes. However, large-scale prospective studies are needed to confirm hs-cTn’s function in diagnosing pulmonary hypertension and determine its potential value in treatment.
Perceived Importance of Types and Characteristics of Support to Informal Caregivers among Spouse Caregivers of Persons with Dementia in Sweden: A Cross-Sectional Questionnaire-Based Study
Authors: Johansson Marcus F. , McKee Kevin J. , Dahlberg Lena , Williams Christine L. , Marmstål Hammar Lena
Published: October 2024

Informal caregivers play a crucial role in the care of individuals with dementia, and their caregiving may significantly impact their own health and well-being. This cross-sectional survey study focuses on the perceived importance of various types and characteristics of formal support in a convenience sample of caregivers aged 65 years or older (N = 175) caring for a spouse with dementia. Participants completed a questionnaire containing 17 items describing different types of support and 12 items describing different characteristics of support, rating their importance. The questionnaire also contained questions on various caregiving-related factors. Principle components analysis (PCA) was carried out on the importance ratings, separately, on the types of support items and the characteristics of support items. Each PCA produced three components. For types of support, they were Proficiency and Opportunity, Supportive Structures, Flexible Counselling. For characteristics of support, they included Respectful and Competent, Timely Support, and Accessible and Acceptable. The three characteristics of the support components all had higher mean importance ratings than the three types of support components. The content of some components indicated that while spouse caregivers rate support for their caregiving needs as important, they may not always differentiate their own needs from those of their partner with dementia. The negative impact of caregiving was the factor most strongly and consistently associated with the components’ importance ratings. This study emphasizes the need for health and social care providers to address the unique needs of spouse caregivers while simultaneously ensuring the delivery of quality care for individuals with dementia.
The Relationship between Components of Postural Control and Locomotive Syndrome in Older Adults
Authors: Lektip Charupa , Rungruangbaiyok Chadapa , Nawarat Jiraphat , Miyake Eiji , Aoki Keiichiro , Ohtsuka Hiroyuki , Inaba Yasuko , Kagaya Yoshinori , Chaovalit Sirawee
Published: October 2024

Locomotive Syndrome (LS), a condition related to impaired mobility, is influenced by balance control, which comprises six components. Deficiencies in these components can lead to reduced mobility and decreased quality of life. This study aimed to evaluate the relationship between the components of postural control and LS in older adults using the Brief-BESTest. Therefore, this cross-sectional study involved 122 elderly participants from Tha Sala District, Nakhon Si Thammarat Province, both with and without LS. Participants underwent assessments using the Instrumental Activities of Daily Living (IADL) assessment, the Thai Mental State Examination (TMSE), the Two-Step Test, and the Brief-BESTest. The Brief-BESTest covers six balance components: Biomechanical Constraints, Stability Limits, Anticipatory Postural Adjustments, Postural Responses, Sensory Orientation, and Stability in Gait. Descriptive statistics were used to summarize participant characteristics, and Chi-square tests were conducted to examine the relationship between each balance component and LS. Cramer’s V was used to assess the strength of the relationships. The results showed the average age of the sample was 67.67 ± 6.01 years with 85.20 percent female and 14.80 percent male. There were significant relationships between LS and three balance components: Biomechanical Constraints (Chi-square = 5.35, p = 0.021, Cramer’s V = 0.209), Stability Limits (Chi-square = 5.00, p = 0.025, Cramer’s V = 0.204), and Anticipatory Postural Adjustments (left: Chi-square = 4.12, p = 0.042, Cramer’s V = 0.213; right: Chi-square = 5.50, p = 0.019, Cramer’s V = 0.213). No significant associations were found for Reactive Postural Response, Sensory Orientation, and Stability in Gait. These findings suggest that targeted interventions focusing on specific balance components consist of Biomechanical Constraints, Stability Limits, and Anticipatory Postural Adjustments could help reduce the risk of LS in older adults.
Financial Innovation and Crowdfunding: Influencing Investment Decisions in Tech Startups
Authors: Panitkulpong Kaimuk , Saengnoree Amnuay , Teerawatananond Thapong
Published: October 2024

This study investigates the financial behavior of Thai investors in equity crowdfunding (ECF), focusing on the factors that influence their investment intentions. Drawing upon the Information System Success Model (ISSM), the Theory of Diffusion of Innovations, and the Technology Acceptance Model 3 (TAM3), the research examines the platform quality (PQ), platform characteristics (PC), and social influence (SI) as independent variables, with the perceived usefulness (PU) and perceived ease of use (PEOU) acting as mediators. Data were gathered from 275 Thai investors and analyzed using Partial Least Squares Structural Equation Modeling (PLS-SEM). The findings reveal that the PU significantly influences investment decisions both directly and indirectly through the PEOU, which also directly affects investment intention. Furthermore, SI, PC, and PQ have indirect effects on investment decisions via the PU and PEOU, with SI being the most influential factor. This study provides valuable insights into optimizing ECF platform design, fostering investor trust, and enhancing regulatory frameworks to facilitate financial inclusion and innovation in the Thai crowdfunding landscape.
Comment on Ioannidou, S.; Pantazis, G. Helmert Transformation Problem. From Euler Angles Method to Quaternion Algebra. ISPRS Int. J. Geo-Inf. 2020, 9, 494
Authors: Bektaş Sebahattin
Published: October 2024

I have read the article by Ioannidou and Pantazis [...]
A Study on a Spatiotemporal Entity-Based Event Data Model
Authors: Wang Mingming , Zhang Jiangshui , Cao Yibing , Li Shenghui , Chen Minjie
Published: October 2024

An event is an important medium for recording, expressing, and understanding the real world. Additionally, a data model can provide a digital and structured description method for the real world. Therefore, studying event data models is highly important for describing the real world. By analyzing the representational categories of the existing event data models, the representation of existing event models was found to have different emphases and not be sufficiently balanced, and the universality and comprehensiveness need to be improved. Therefore, based on the advantages of the ontological event model in expressing semantic information and the advantages of the object-event-based spatiotemporal data model in expressing entity multidimensional characteristics and dynamic processes, a spatiotemporal entity-based event data model and the modeling method were designed to provide model support for event organization and processing. Additionally, the Long March and its important battles were selected as case studies to validate the proposed model. The validation shows that the proposed model performs well in terms of event dynamics, hierarchical structure, and complex interrelationships.
A New Framework for Integrating DNN-Based Geographic Simulation Models within GISystems
Authors: Zhang Peng , Wu Wenzhou , Xue Cunjin , Shi Shaochen , Su Fenzhen
Published: October 2024

As a crucial spatial decision support tool, Geographic Information Systems (GISystems) are widely used in fields such as digital watersheds, resource management, environmental assessment, and regional governance, with their core strength lying in the integration of geographic simulation models from various disciplines, enabling the analysis of complex geographical phenomena and the resolution of comprehensive spatial problems. With the rapid advancement of artificial intelligence, deep neural network-based geographic simulation models (DNN-GSMs) have increasingly replaced traditional models, offering significant advantages in simulation accuracy and inference speed, and have become indispensable components in GISystems. However, existing integration methods do not adequately account for the specific characteristics of DNN-GSMs, such as their formats and input/output data types. To address this gap, we propose a novel tight integration framework for DNN-GSMs, comprising four key interfaces: the data representation interface, the model representation interface, the data conversion interface, and the model application interface. These interfaces are designed to describe spatial data, the simulation model, the adaptation between spatial data and the model, and the model’s application process within the GISystem, respectively. To validate the proposed method, we construct a spatial morphology simulation model based on CNN-LSTM, integrate it into a GISystem using the proposed interfaces, and conduct a series of predictive experiments on island morphology evolution. The results demonstrate the effectiveness of the proposed integration framework for DNN-GSMs.
B Cell Lymphoma 6 (BCL6): A Conserved Regulator of Immunity and Beyond
Authors: Liongue Clifford , Almohaisen Farooq L. J. , Ward Alister C.
Published: October 2024

B cell lymphoma 6 (BCL6) is a conserved multi-domain protein that functions principally as a transcriptional repressor. This protein regulates many pivotal aspects of immune cell development and function. BCL6 is critical for germinal center (GC) formation and the development of high-affinity antibodies, with key roles in the generation and function of GC B cells, follicular helper T (Tfh) cells, follicular regulatory T (Tfr) cells, and various immune memory cells. BCL6 also controls macrophage production and function as well as performing a myriad of additional roles outside of the immune system. Many of these regulatory functions are conserved throughout evolution. The BCL6 gene is also important in human oncology, particularly in diffuse large B cell lymphoma (DLBCL) and follicular lymphoma (FL), but also extending to many in other cancers, including a unique role in resistance to a variety of therapies, which collectively make BCL6 inhibitors highly sought-after.
Effect of Sprouting, Fermentation and Cooking on Antioxidant Content and Total Antioxidant Activity in Quinoa and Amaranth
Authors: Vento Martina , Della Croce Clara Maria , Bellani Lorenza , Tassi Eliana Lanfranca , Echeverria Maria Cristina , Giorgetti Lucia
Published: October 2024

The study of different processing techniques, such as sprouting, cooking and fermentation, can help to develop new products for human health. In this work, raw, cooked and fermented seeds and germinated seeds of Chenopodium quinoa Willd. var. Tunkahuan and Amaranthus caudatus L. var. Alegrìa were compared for the content of antioxidant molecules, total antioxidant capacity and mineral elements. Fermentation was induced spontaneously, with the yeast Saccharomyces cerevisiae, with the bacterium Lactobacillus plantarum and with both microorganisms, for 24 and 48 h. The increase in antioxidant molecules and antioxidant activity was induced by germination, by 24 h of spontaneous fermentation (polyphenols and flavonoids) and by 24 h of L. plantarum fermentation (total antioxidant activity) for both species. Germinated seeds of the two plants showed higher values in respect to seeds of macroelements and microelements. No genotoxic but rather protective effects were determined for seed and germinated seed extracts using the D7 strain of S. cerevisiae, a good tool for the evaluation of protection from oxidative damage induced by radical oxygen species (ROS) in cells and tissues. Therefore, the two varieties could be very suitable for their use in human diet and in supplements, especially as germinated seeds or as fermented foods.
Mitochondrial Dysfunction as a Potential Mechanism Mediating Cardiac Comorbidities in Parkinson’s Disease
Authors: Salis Torres Agustina , Lee Ji-Eun , Caporali Andrea , Semple Robert K. , Horrocks Mathew H. , MacRae Vicky E.
Published: October 2024

Individuals diagnosed with Parkinson’s disease (PD) often exhibit heightened susceptibility to cardiac dysfunction, reflecting a complex interaction between these conditions. The involvement of mitochondrial dysfunction in the development and progression of cardiac dysfunction and PD suggests a plausible commonality in some aspects of their molecular pathogenesis, potentially contributing to the prevalence of cardiac issues in PD. Mitochondria, crucial organelles responsible for energy production and cellular regulation, play important roles in tissues with high energetic demands, such as neurons and cardiac cells. Mitochondrial dysfunction can occur in different and non-mutually exclusive ways; however, some mechanisms include alterations in mitochondrial dynamics, compromised bioenergetics, biogenesis deficits, oxidative stress, impaired mitophagy, and disrupted calcium balance. It is plausible that these factors contribute to the increased prevalence of cardiac dysfunction in PD, suggesting mitochondrial health as a potential target for therapeutic intervention. This review provides an overview of the physiological mechanisms underlying mitochondrial quality control systems. It summarises the diverse roles of mitochondria in brain and heart function, highlighting shared pathways potentially exhibiting dysfunction and driving cardiac comorbidities in PD. By highlighting strategies to mitigate dysfunction associated with mitochondrial impairment in cardiac and neural tissues, our review aims to provide new perspectives on therapeutic approaches.
Cardioprotection by Preconditioning with Intralipid Is Sustained in a Model of Endothelial Dysfunction for Isolated-Perfused Hearts
Authors: Stroethoff Martin , Schneider Natalie , Sung Lea , Wübbolt Jan , Heinen André , Raupach Annika
Published: October 2024

Endothelial dysfunction (ED) is closely associated with most cardiovascular diseases. Experimental models are needed to analyze the potential impact of ED on cardioprotection in constant pressure Langendorff systems (CPLS). One cardioprotective strategy against ischemia/reperfusion injury (I/RI) is conditioning with the lipid emulsion Intralipid (IL). Whether ED modulates the cardioprotective effect of IL remains unknown. The aim of the study was to transfer a protocol using a constant flow Langendorff system for the induction of ED into a CPLS, without the loss of smooth muscle cell functionality, and to analyze the cardioprotective effect of IL against I/RI under ED. In isolated hearts of male Wistar rats, ED was induced by 10 min perfusion of a Krebs–Henseleit buffer containing 60 mM KCl (K+), and the vasodilatory response to the vasodilators histamine (endothelial-dependent) and sodium–nitroprusside (SNP, endothelial-independent) was measured. A CPLS was employed to determine cardioprotection of pre- or postconditioning with 1% IL against I/RI. The constant flow perfusion of K+ reduced endothelial response to histamine but not to SNP, indicating reduced vasodilatory functionality of endothelial cells but not smooth muscle cells. Preconditioning with IL reduced infarct size and improved cardiac function while postconditioning with IL had no effect. The induction of ED neither influenced infarct size nor affected the cardioprotective effect by preconditioning with IL. This protocol allows for studies of cardioprotective strategies under ED in CLPS. The protection by preconditioning with IL seems to be mediated independently of a functional endothelium.
Encapsulation of β-Galactosidase into Polyallylamine/Polystyrene Sulphonate Polyelectrolyte Microcapsules
Authors: Chebykin Yuri S. , Musin Egor V. , Kim Aleksandr L. , Tikhonenko Sergey A.
Published: October 2024

More than half of the global population is unable to consume dairy products due to lactose intolerance (hypolactasia). Current enzyme replacement therapy methods are insufficiently effective as a therapeutic approach to treating lactose intolerance. The encapsulation of β-galactosidase in polyelectrolyte microcapsules by using the layer-by-layer method could be a possible solution to this problem. In this study, adsorption and co-precipitation methods were employed for encapsulating β-galactosidase in polyelectrolyte microcapsules composed of (polyallylamine /polystyrene sulphonate)₃. As a result, the co-precipitation method was chosen for β-galactosidase encapsulation. The adsorption method permits to encapsulate six times less enzyme compared with the co-precipitation method; the β-galactosidase encapsulated via the co-precipitation method released no more than 20% of the initially encapsulated enzyme in pH 2 or 1 M NaCl solutions. In contrast, when using the sorption method, about 100% of the initially encapsulated enzyme was released from the microcapsules under the conditions described above. The co-precipitation method effectively prevents the complete loss of enzyme activity after 2 h of incubation in a solution with pH 2 while also alleviating the adverse effects of ionic strength. Consequently, the encapsulated form of β-galactosidase shows promise as a potential therapeutic agent for enzyme replacement therapy in the treatment of hypolactasia.