Characteristics of tick-borne encephalitis in vaccinated patients and criteria for specific laboratory diagnostics
Authors:   M.P.Chumakov Federal Research Center for the Development of Immune and Biological Products, Russian Academy of Sciences, Moscow, Russian Federation, Pogodina V.V. , Shcherbinina M.S. ,   Research Center for Expert Evaluation of Medicinal Product, Moscow, Russian Federation, Semenov V.A. ,   Kemerovo State Medical University, Kemerovo, Russian Federation, Bochkova N.G. , Ishmukhametov A.A. ,   M.P.Chumakov Federal Research Center for the Development of Immune and Biological Products, Russian Academy of Sciences, Moscow, Russian Federation, Maleev V.V. ,   Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russian Federation, Gerasimov S.G. ,   M.P.Chumakov Federal Research Center for the Development of Immune and Biological Products, Russian Academy of Sciences, Moscow, Russian Federation,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Kolyasnikova N.M. ,   M.P.Chumakov Federal Research Center for the Development of Immune and Biological Products, Russian Academy of Sciences, Moscow, Russian Federation,   Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russian Federation
Published: March 2021

Currently available vaccines against tick-borne encephalitis (TBE) are highly effective; however, they do not ensure 100% protection. Laboratory diagnostics of TBE in vaccinated patients remains poorly developed. Objective. To compare the dynamics of humoral immunity in unvaccinated and vaccinated patients and to develop an algorithm of specific laboratory diagnostics of TBE. Patients and methods. We have tested 276 unvaccinated and 59 vaccinated patients using enzyme-linked immunosorbent assay for the detection of IgG and IgM against antigens of tick-borne encephalitis virus (TBEV). In hemagglutination inhibition assay, we used dry TBEV reagent. TBEV RNA was detected using reverse transcription polymerase chain reaction (RT-PCR). Results. We have identified three types of humoral immunity dynamics in vaccinated and unvaccinated patients: seroconversion, stable antibody titers, and negative (reverse) antibody dynamics with an at least 4-fold decrease in antibody levels. Among 39 vaccinated individuals, 18 patients demonstrated seroconversion, while 16 patients had stable antibody titers and 4 patients had negative dynamics. Patients with chronic TBE demonstrated different variants of humoral immunity dynamics. Discussion. We discuss the variants of antibody dynamics described above and diagnostic methods on the example of vaccinated patients. In individuals with stable antibody titers, we used an original method for detecting the dynamics of antibodies against Siberian strain of TBEV. Conclusion. In case of seroconversion, the level of specific immunity in vaccinated patients was higher than that in unvaccinated patients and healthy individuals vaccinated against TBE. Negative antibody dynamics is the rarest variant of humoral immunity development. The diagnosis of TBE in patients with stable antibody titers is confirmed by the detection of TBEV antigen, increasing serum levels of IgM, detection of TBEV RNA in the cerebrospinal fluid, and isolation of the virus. Keywords: vaccinated patients, humoral immunity, diagnosis, tick-borne encephalitis