bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Factors for Endometriosis Among Egyptian Infertile Women with Different Disease Stages
Authors: Abd el-Kader Azza Ibrahim , Gonied Amina Saad , Mohamed Mohamed Lotfy , Mohamed Sabah Lotfy
Published: July 2022

Background

Endometriosis is regarded as a benign condition, despite the potential for recurrence and metastasis,. It might lead to secondary ovarian cancer from endometrioma.

Objective

The aim of this current study was to determine the relationship between risk factors for endometriosis and disease stages.

Methods

This current cross-sectional study was performed at the endoscopic unit at Zagazig University Hospitals, Egypt. The participants included 85 women who were candidates for laparoscopy and diagnosed with endometriosis in the period from November 2019 to November 2020. The tools used for data collection were a structured interviewing form and the American Society of Reproductive Medicine scoring tool (ASRM) for determining stages of endometriosis.

Results

The high percentages of studied women were nulliparous (74.1%) and (51.8%) have secondary infertility. Endometriosis was staged as 20.0% minimal (ASRM stage I), 34.1% mild (ASRM stage II), 29.4% moderate (ASRM stage III), and 16.5% severe (ASRM stage IV). Congestive dysmenorrhea (78.8%), dyspareunia (77.6%), and secondary infertility (51.8%) were more reported symptoms in all stages of endometriosis.

Conclusion

This study concluded that women within reproductive age, nulliparity, lower body weight, urban residence, and past surgery of the pelvic were considered as risk factors in all disease stages.
Water and Dalits: A Historical Study of United Provinces (1900Ð1950)
Authors: Swaroop Arvind
Published: July 2022

Water has been a critical resource for the survival of the biotic world. This paper explores the relationship between Dalits and access to water in the United Provinces. It documents the social condition and economic status of Dalits and their impact on livelihood structure. The fact is that the Dalit community is dependent critically on water and other natural resources due to its involvement with labour and agriculture. This paper seeks to explore the historical dimension of Dalit’s struggle to gain access to water in the United Provinces. The history of the relationship between water and Dalits in the United Provinces shows the way social exclusion manifested in the domain of access to water. This paper has been divided into four sections, the first section provides general information about Dalits, and the second section proposes a brief review of the literature. The third section attempts to trace the relationship between water and Dalits in the process of exclusion and inclusion of Dalits from natural resources in North India and the final section provides an illation of this paper.
Synergistic Effects of Natural Product Combinations in Protecting the Endothelium Against Cardiovascular Risk Factors
Authors: Yousaf Muhammad , Razmovski-Naumovski Valentina , Zubair Muhammad , Chang Dennis , Zhou Xian
Published: July 2022

Endothelial dysfunction is an early hallmark of cardiovascular diseases (CVDs). Monotherapies are limited due to the complex, multifactorial pathways. The multi-component and multi-targeted approach of natural products have the potential to manage CVDs.

This review aims to provide a comprehensive insight into the synergistic mechanism of natural product combinations in protecting the endothelium against various cardiovascular risk factors.

Databases (PubMed, MEDLINE and EMBASE) and Google Scholar were searched, and studies in English published between January 2000 and February 2022 were collated. Clinical and pre-clinical studies of natural product combinations with or without pharmaceutical medicines, compared with monotherapy and/or proposing the underlying mechanism in protecting endothelial function, were included.

Four clinical studies demonstrated that natural product combinations or natural product-pharmaceutical combinations improved endothelial function. This was associated with multi-targeted effects or improved absorption of the active substances in the body. Seventeen preclinical studies showed that natural product combinations produced synergistic (demonstrated by combination index or Bliss independence model) or enhanced effects in protecting the endothelium against hyperlipidemia, hypertension, diabetes mellitus, platelet activation, oxidative stress and hyperhomocysteinemia. The molecular targets included reactive oxygen species, Nrf2-HO-1, p38MAPK, P13K/Akt and NF-κB.

Thus, the current available evidence of natural product combinations in targeting endothelial dysfunction is predominantly from preclinical studies. These have demonstrated synergistic/enhanced pharmacological activities and proposed associated mechanisms. However, evidence from larger, well-designed clinical trials remains weak. More cohesion is required between preclinical and clinical data to support natural product combinations in preventing or slowing the progression of CVDs.
The effects of loading ranibizumab on vision-related quality of life in the treatment of low-risk neovascular age-related macular degeneration
Authors: Daldal Hatice
Published: July 2022

Background:

The loss of central vision plays a major role in the quality of life (QoL). This study evaluates the changes in QoL in the treatment of low-risk neovascular age-related macular degeneration (AMD).

Objectives:

The aim of this study was to evaluate the changes in vision-related QoL in patients receiving intravitreal ranibizumab loading dose for low-risk neovascular AMD.

Design:

A prospective study.

Methods:

Forty-two eyes of 42 patients receiving ranibizumab injections for neovascular AMD were included in this prospective study. The changes in best-corrected visual acuity (BCVA), central macular thickness (CMT) of the patients before the treatment, and 1 month after three loading doses were evaluated. Turkish version of the National Eye Institute 25-Item Vision Function Questionnaire (NEI VFQ-25 TR) was conducted. The changes of QoL scores through the NEI VFQ-25 TR questionnaire were compared with visual acuity (VA) and CMT measurements before the injections and 1 month after the loading dose.

Results:

Forty-two patients (19 females and 23 males) were included in the study, and the mean age was 72.69 ± 7.12 years. After the treatment, a statistically significant improvement in BCVA (0.98 ± 0.44, 0.76 ± 0.42 logMAR, p < 0.001) and a significant decrease in CMT (357.90 ± 71.71, 274.50 ± 58.35 μm, p < 0.001) was observed. The QoL composite score was found to be statistically significantly higher after the treatment (64.27 ± 11.47, 68.56 ± 11.39, p < 0.001). General vision ( p < 0.001), ocular pain ( p = 0.025), near activities ( p < 0.001), distance activities ( p = 0.027), vision-specific mental health ( p = 0.014), vision-specific role difficulties ( p < 0.001), and peripheral vision ( p = 0.046) were significantly higher after the treatment.

Conclusion:

NEI VFQ-25 TR is a useful questionnaire for evaluating changes in visual functions and psychosocial characteristics of low-risk neovascular AMD patients before and after the injections.
Evaluation of Pediatric Facial Fracture Patients during the COVID-19 Pandemic
Authors: Irgebay Zhazira , Beiriger Justin W. , Mak Allison K. , Kueper Janina K. , Smetona John , Losee Joseph E. , Goldstein Jesse A.
Published: July 2022

Background:

The COVID-19 pandemic has significantly impacted the provision of health care, including the suspension of elective and nonessential surgeries. This study presents an early effect of the COVID-19 pandemic on the surgical care of pediatric patients with facial fractures.

Methods:

This study presents a retrospective cohort study of pediatric patients with facial fractures who presented to a pediatric level I trauma center before and during the pandemic. Patients were included regardless of treating specialty, treatment modality, or inpatient status.

Results:

One hundred nine patients met the inclusion criteria. There were 70 patients in the pre-pandemic cohort and 39 in the pandemic cohort. Mean age in the pre-pandemic cohort was 11.6 ± 4.5 years old and 11.1 ± 4.7 in the pandemic cohort ( P = .623). Majority of patients during the pandemic were female (59%), compared to the predominantly male pre-pandemic cohort (25.7%). Motor vehicle accidents were the most common cause of injury during the pandemic (n = 16, 41.0%), compared to sports in pre-pandemic (n = 21, 30.0%). The most common fracture during the pandemic was nasal fracture (n = 26, 66.7%), which was also the most common fracture in pre-pandemic cohort (n = 59, 84.3%). There were significantly more skull fractures (17.9%, P = .02) and orbital fractures (23.1%, P = .02) during the pandemic, as well as more hospital (33.0%, P = .004) and ICU admissions (17.9%, P = .018). There was no difference in operative rate during the pandemic (41.0%) compared to pre-pandemic (48.6%, P = .449).

Conclusions:

The COVID-19 pandemic caused a global stoppage of sports and outdoor activities. As a result, more of the fractures that presented to the hospital were due to motor vehicle accidents during the pandemic, leading to proportionally greater rates of admissions and neurological associated injuries.
A Symbolic Interactionist Approach to Interpreter’s Identity Management
Authors: Yuan Xiaohui
Published: July 2022

A lacuna in interpreting studies pertains to what constitutes an interpreter’s identities in social and sociopsychological contexts and how they operate, influencing the interpreter’s decision-making and behaviours at interpreting-facilitated events. In this research, two key symbolic interactionism frameworks, structural symbolic interactionism (SSI) and perceptual control theory, are drawn on to formulate a symbolic interactionist model of interpreter’s identity management (SIMIIM) with a view to investigating higher socio-structural and individual psychological influence on an interpreter’s identity management. Interpretative Phenomenological Analysis (IPA) of a professional public service interpreter’s perceptions of her lived experience of interpreting and the sense of self is undertaken to illustrate the key elements of the model. Issues regarding moral identity, professional identity, social and communicative factors challenging the interpreter’s identity management, and impacts of identity nonverification on the interpreter’s wellbeing are discussed. Introducing symbolic interactionism to interpreting studies for the first time, it is hoped that this research will encourage further efforts to explore an interpreter’s identity management at social and individual levels, which is key to an informed understanding of the interpreter’s behaviour and of the issues of interpreting ethics in public service interpreting.
Modelling connection trips to long-distance travel
Authors: Kristoffersson Ida , Berglund Svante
Published: July 2022

Connection trips is often an important part of long-distance travel, especially for air travel. Models of long-distance travel would therefore benefit from a more detailed representation of the connection part. In this paper it is however shown that most models of connection trips are stand-alone models not integrated with the model for main mode. Only a handful models that integrate connection trip modelling into a large-scale transport model for long-distance travel are found. The connection trip models are classified into different types using a typology developed within the paper. The typology identifies nine model types that differ in how access/egress mode choice and terminal choice are handled. The scarce literature on connection trip modelling within large-scale transport modelling systems call for more research regarding detailed representation of access/egress mode choice and terminal choice, especially regarding the trade-off between model complexity and detailed representation, as well as whether the detailed representation of connection trips should primarily be conducted within the public transport network assignment or on the demand modelling side.
MicroRNA-mediated host defense mechanisms against pathogens and herbivores in rice: balancing gains from genetic resistance with trade-offs to productivity potential
Authors: Kumar Kishor , Mandal Swarupa Nanda , Neelam Kumari , de los Reyes Benildo G.
Published: July 2022

Background

Rice (Oryza sativa L.) is the major source of daily caloric intake for more than 30% of the human population. However, the sustained productivity of this staple food crop is continuously threatened by various pathogens and herbivores. Breeding has been successful in utilizing various mechanisms of defense by gene pyramiding in elite cultivars, but the continuous resurgence of highly resistant races of pathogens and herbivores often overcomes the inherent capacity of host plant immunity. MicroRNAs (miRNAs) are endogenous, short, single-stranded, non-coding RNA molecules that regulate gene expression by sequence-specific cleavage of target mRNA or suppressing target mRNA translation. While miRNAs function as upstream regulators of plant growth, development, and host immunity, their direct effects on growth and development in the context of balancing defenses with agronomic potential have not been extensively discussed and explored as a more viable strategy in breeding for disease and pest resistant cultivars of rice with optimal agronomic potentials.

Results

Using the available knowledge in rice and other model plants, this review examines the important roles of miRNAs in regulating host responses to various fungal, bacterial, and viral pathogens, and insect pests, in the context of gains and trade-offs to crop yield. Gains from R-gene-mediated resistance deployed in modern rice cultivars are often undermined by the rapid breakdown of resistance, negative pleiotropic effects, and linkage drags with undesirable traits. In stark contrast, several classes of miRNAs are known to efficiently balance the positive gains from host immunity without significant costs in terms of losses in agronomic potentials (i.e., yield penalty) in rice. Defense-related miRNAs such as Osa-miR156, Osa-miR159, Osa-miR162, Osa-miR396, Osa-530, Osa-miR1432, Osa-miR1871, and Osa-miR1873 are critical in fine-tuning and integrating immune responses with physiological processes that are necessary to the maintenance of grain yield. Recent research has shown that many defense-related miRNAs regulate complex and agronomically important traits.

Conclusions

Identification of novel immune-responsive miRNAs that orchestrate physiological processes critical to the full expression of agronomic potential will facilitate the stacking of optimal combinations of miRNA-encoding genes to develop high-yielding cultivars with durable resistance to disease and insect pests with minimal penalties to yield.
Ginkgolide B improved postoperative cognitive dysfunction by inhibiting microgliosis-mediated neuroinflammation in the hippocampus of mice
Authors: Luo Ting , Hao Ya-Nan , Lin Dan-Dan , Huang Xiao , Wu An-Shi
Published: July 2022

Background

Postoperative cognitive dysfunction (POCD) are a common complication of the central nervous system following surgery and anesthesia. The specific pathogenesis and effective therapeutics of POCD need to be further studied. Ginkgolide B (GB), a platelet-activating factor receptor-specific antagonist, has been suggested to have strong anti-inflammatory effects. Here we tested the effects and mechanism of GB on POCD of aged rats.

Methods

Neurobehavioral tests were used to investigate the effect of GB pretreatment on POCD. The hippocampus were harvested to test the expression of proinflammatory cytokines by ELISA. The expression of the microglial marker ionized calcium-binding adaptor molecule-1 (Iba-1) in the hippocampus was evaluated by western blot assay and immunohistochemistry. A Nissl staining experiment was used to detect the neuronal numbers in the hippocampus.

Results

Surgery might result in the overexpression of platelet activating factor (PAF) in the plasma and hippocampus and might cause hippocampus-dependent memory impairment. GB pretreatment, inhibited the activation of microglia, reduced the levels of IL-1β and TNF-α, decreased the loss of neurons after surgery, and prevented POCD in aged rats.

Conclusion

Our findings suggested that PAF was involved in the development of POCD. Improvement of POCD by PAF antagonist GB was associated with the inhibition of microgliosis-mediated neuroinflammation and neuronal apoptosis in aged rats.
Exploration of dilated cardiomyopathy for biomarkers and immune microenvironment: evidence from RNA-seq
Authors: Fang Chenggang , Lv Zhan , Yu Zhimin , Wang Kexin , Xu Chengkai , Li Yixuan , Wang Yanggan
Published: July 2022

Background

The pathogenic mechanism of dilated cardiomyopathy (DCM) remains to be defined. This study aimed to identify hub genes and immune cells that could serve as potential therapeutic targets for DCM.

Methods

We downloaded four datasets from the Gene Expression Omnibus (GEO) database: GSE141910, GSE3585, GSE42955 and GSE79962. Weighted gene coexpression network analysis (WGCNA) and differential expression analysis were performed to identify gene panels related to DCM. Meanwhile, the CIBERSORT algorithm was used to estimate the immune cells in DCM tissues. Multiple machine learning approaches were used to screen the hub genes and immune cells. Finally, the diagnostic value of the hub genes was assessed by receiver operating characteristic (ROC) analysis. An experimental mouse model of dilated cardiomyopathy was used to validate the bioinformatics results.

Results

FRZB and EXT1 were identified as hub biomarkers, and the ROC curves suggested an excellent diagnostic ability of the above genes for DCM. In addition, naive B cells were upregulated in DCM tissues, while eosinophils, M2 macrophages, and memory CD4 T cells were downregulated in DCM tissues. The increase in two hub genes and naive B cells was validated in animal experiments.

Conclusion

These results indicated that FRZB and EXT1 could be used as promising biomarkers, and eosinophils, M2 macrophages, resting memory CD4 T cells and naive B cells may also affect the occurrence of DCM.
Challenges in abdominal re-exploration for war casualties following on-site abdominal trauma surgery and subsequent delayed arrival to definitive medical care abroad – an unusual scenario
Authors: Bickel Amitai , Akinichev Konstantin , Weiss Michael , Ganam Samer , Biswas Seema , Waksman Igor , Kakiashvilli Eli
Published: July 2022

Background

During the Syrian civil war, patients were initially treated on-site in Syria and later transferred to medical centers in Israel. Relevant details concerning the exact nature of injury and medical/surgical care received in Syria were unavailable to clinicians in Israel. Many of these patients required abdominal re-exploration for obvious or suspected problems related to their injury. Our aim is to present our approach to abdominal trauma patients who survived initial on-site surgery and needed subsequent abdominal re-exploration abroad, in our medical center.

Methods

Clinical data from all medical records were retrospectively analyzed. Each patient underwent total body computerized tomography on arrival, revealing diverse multi-organ trauma. We divided the patient population who had abdominal trauma into 4 sub-groups according to the location in which abdominal surgical intervention was performed (abdominal surgery performed only in Syria, surgery in Syria and subsequent re-laparotomy in Israel, abdominal surgery only in Israel, and management of patients without abdominal surgical intervention). We focused on missed injuries and post-operative complications in the re-laparotomy sub-group.

Results

By July 2018, 1331 trauma patients had been admitted to our hospital, of whom 236 had suffered abdominal trauma. Life-saving abdominal intervention was performed in 138 patients in Syria before arrival to our medical center.

A total of 79 patients underwent abdominal surgery in Israel, of whom 46 (33%) required re-laparotomy. The absence of any communication between the surgical teams across the border markedly affected our medical approach. Indications for re-exploration included severe peritoneal inflammation, neglected or overlooked abdominal foreign bodies, hemodynamic instability and intestinal fistula. Mortality occurred in 37/236 patients, with severe abdominal trauma as the main cause of fatality in 10 of them (4.2%), usually following urgent re-laparotomy.

Conclusions

Lack of information about the circumstances of injury in an environment of catastrophe in Syria at the time and the absence of professional communication between the surgical teams across the border markedly dictated our medical approach. Our concerns were that some patients looked deceptively stable while others had potentially hidden injuries. We had no information on who had had definitive versus damage control surgery in Syria. The fact that re-operation was not performed by the same team responsible for initial abdominal intervention also posed major diagnostic challenges and warranted increased clinical suspicion and a change in our standard medical approach.
The effect of high prevalence of missing data on estimation of the coefficients of a logistic regression model when using multiple imputation
Authors: Austin Peter C. , van Buuren Stef
Published: July 2022

Background

Multiple imputation is frequently used to address missing data when conducting statistical analyses. There is a paucity of research into the performance of multiple imputation when the prevalence of missing data is very high. Our objective was to assess the performance of multiple imputation when estimating a logistic regression model when the prevalence of missing data for predictor variables is very high.

Methods

Monte Carlo simulations were used to examine the performance of multiple imputation when estimating a multivariable logistic regression model. We varied the size of the analysis samples (N = 500, 1,000, 5,000, 10,000, and 25,000) and the prevalence of missing data (5–95% in increments of 5%).

Results

In general, multiple imputation performed well across the range of scenarios. The exceptions were in scenarios when the sample size was 500 or 1,000 and the prevalence of missing data was at least 90%. In these scenarios, the estimated standard errors of the log-odds ratios were very large and did not accurately estimate the standard deviation of the sampling distribution of the log-odds ratio. Furthermore, in these settings, estimated confidence intervals tended to be conservative. In all other settings (i.e., sample sizes > 1,000 or when the prevalence of missing data was less than 90%), then multiple imputation allowed for accurate estimation of a logistic regression model.

Conclusions

Multiple imputation can be used in many scenarios with a very high prevalence of missing data.
Can the WHO ‘s recommendations of physical activity volume decrease the risk of heart disease in middle and older aged Chinese People: the evidence from a seven year longitudinal survey
Authors: Ding Meng , Zhou Yanan , Li Chengxiang , Li Weipeng , Jia Ningxin , Dong Xiaosheng
Published: July 2022

Background

At present, there is a lack of direct evidence to confirm whether the recommendations of the World Health Organization can play a role in fitness and disease prevention in the Chinese population. Therefore, we aimed to analyse 7-year longitudinal survey data to explore whether the physical activity volume recommended by the World Health Organization can help Chinese middle-aged and elderly people reduce the risk of heart disease.

Methods

Data for the 8327 participants who were finally included in this study were derived from the 7-year data of the China Health and Retirement Longitudinal Study (CHARLS) from 2011 to 2018. The physical activity volume is expressed by the product of physical frequency and duration, and heart disease is screened according to self-reported diagnosis and related treatment. The relationship between different physical activity volume groups and the incidence rate of heart disease was determined by a multivariate Cox proportional hazards regression model.

Results

After adjusting for all covariates, participants meeting the WHO’s recommendations had a 20% lower risk of heart disease than those who did not meet the WHO’s recommendations (HR = 0.80, 95% = 0.68–0.96). Subgroup analysis showed that among the participants meeting the WHO’s recommendations, men (HR = 0.71) had a lower risk of heart disease than women (HR = 0.74); in addition, the risk of heart disease was significantly reduced in participants who were middle-aged (26%), had a normal BMI range (49%), did not have hypertension (24%), did not have hyperlipidaemia (21%) and did not have lung disease (21%). It should be noted that the risk of heart disease was reduced by 72 and 67% in participants with untreated hyperlipidaemia and untreated lung disease, respectively.

Conclusions

This study revealed that meeting the WHO’s recommendations for physical activity volume can reduce the risk of heart disease in middle-aged and older people in China and can also effectively prevent heart disease for people with some common chronic diseases, such as hyperlipidaemia and lung disease. The results showed that physical activity for leisure and exercise had a lower preventive effect on heart disease than physical activity for a job, which may be related to the inappropriate leisure and exercise methods of the participants.

Trial registration

IRB00001052–11015.
The importin beta superfamily member RanBP17 exhibits a role in cell proliferation and is associated with improved survival of patients with HPV+ HNSCC
Authors: Mandic Robert , Marquardt André , Terhorst Philip , Ali Uzma , Nowak-Rossmann Annette , Cai Chengzhong , Rodepeter Fiona R. , Stiewe Thorsten , Wezorke Bernadette , Wanzel Michael , Neff Andreas , Stuck Boris A. , Bette Michael
Published: July 2022

Background

More than twenty years after its discovery, the role of the importin beta superfamily member Ran GTP-binding protein (RanBP) 17 is still ill defined. Previously, we observed notable RanBP17 RNA expression levels in head and neck squamous cell carcinoma (HNSCC) cell lines with disruptive TP53 mutations.

Methods

We deployed HNSCC cell lines as well as cell lines from other tumor entities such as HCT116, MDA-MB-231 and H460, which were derived from colon, breast and lung cancers respectively. RNAi was used to evaluate the effect of RanBP17 on cell proliferation. FACS analysis was used for cell sorting according to their respective cell cycle phase and for BrdU assays. Immunocytochemistry was deployed for colocalization studies of RanBP17 with Nucleolin and SC35 (nuclear speckles) domains. TCGA analysis was performed for prognostic assessment and correlation analysis of RanBP17 in HNSCC patients.

Results

RNAi knockdown of RanBP17, significantly reduced cell proliferation in HNSCC cell lines. This effect was also seen in the HNSCC unrelated cell lines HCT116 and MDA-MB-231. Similarly, inhibiting cell proliferation with cisplatin reduced RanBP17 in keratinocytes but lead to induction in tumor cell lines. A similar observation was made in tumor cell lines after treatment with the EGFR kinase inhibitor AG1478. In addition to previous reports, showing colocalization of RanBP17 with SC35 domains, we observed colocalization of RanBP17 to nuclear bodies that are distinct from nucleoli and SC35 domains. Interestingly, for HPV positive but not HPV negative HNSCC, TCGA data base analysis revealed a strong positive correlation of RanBP17 RNA with patient survival and CDKN2A.

Conclusions

Our data point to a role of RanBP17 in proliferation of HNSCC and other epithelial cells. Furthermore, RanBP17 could potentially serve as a novel prognostic marker for HNSCC patients. However, we noted a major discrepancy between RanBP17 RNA and protein expression levels with the used antibodies. These observations could be explained by the presence of additional RanBP17 splice isoforms and more so of non-coding circular RanBP17 RNA species. These aspects need to be addressed in more detail by future studies.
Incidence trends of childhood central nervous system tumors in Finland 1990–2017
Authors: Abuhamed Jad , Nikkilä Atte , Raitanen Jani , Alimam Wafa , Lohi Olli , Pitkäniemi Janne , Haapasalo Hannu , Auvinen Anssi
Published: July 2022

Introduction

Central nervous system (CNS) tumors are a leading cause of cancer-related morbidity and mortality in children. Our aim is to characterize incidence trends of pediatric CNS tumors in Finland over the last three decades.

Methods

Data on all benign and malignant incident CNS tumors diagnosed in children aged 0–14 years in 1990–2017 were extracted from the Finnish Cancer Registry and classified according to the 2016 WHO classification of CNS tumors. We analyzed age-standardized incidence rates (ASR) for pediatric CNS tumors overall and by sex, age, tumor histology, grade, and location using Poisson regression. We used joinpoint regression to evaluate changes in trends.

Results

Overall, 1117 pediatric CNS tumor cases were registered in Finland with a 1.2:1 male to female ratio. The average annual ASR was 4.3 per 100,000 person-years (95% CI 4.26, 4.34). The most common tumor type was pilocytic astrocytoma (30% of tumors), followed by medulloblastoma (10%) with incidence rates of 1.30 and 0.45 per 100,000 person-years, respectively. The overall incidence of pediatric CNS tumors increased by an annual percentage change (APC) of 0.8% (95% CI 0.2, 1.4). We observed no major changes in incidence trends of tumor histology groups or tumor location groups. The ASR of benign tumors increased by an APC of 1.0 (95% CI 0.1, 2.0).

Conclusions

Utilizing the high-quality and completeness of data in the Finnish Cancer registry, we found that the incidence of pediatric CNS tumors in Finland has increased slightly from 1990 until 2017. Although variations in diagnostic and registration practices over time might have affected the rates, the trend may also reflect a true increase in incidence.
Choroidal and retinal thickness in patients with vitamin C deficiency using swept-source optical coherence tomography
Authors: Qian Yiwen , Wang Luoziyi , Qiang Xinfang , Weng Huan , Jiang Jing , Che Xin , Li Qingjian , Wang Zhiliang
Published: July 2022

Background

To investigate the effects of vitamin C on central retinal thickness and choroidal thickness.

Methods

A total of 69 patients diagnosed with vitamin C deficiency and 1:1 age- and gender-matched 69 healthy individuals with normal serum vitamin C were included in this study. Demographic characteristics of the individuals were collected. All patients underwent a comprehensive ophthalmic examination. Subfoveal choroidal thickness and retinal thickness were measured using a swept-source optical coherence tomography (SS-OCT).

Results

The average retinal thickness was 269.07 ± 13.51 μm in the vitamin C deficiency group and 276.92 ± 13.51 μm in the control group. The average choroidal thickness was 195.62 ± 66.40 μm in the in the vitamin C deficiency group and 238.86 ± 55.08 μm in the control group. There was a significant decrease in both average choroidal thickness and retinal thickness in vitamin C deficiency group compared with normal individuals (p < 0.001, and = 0.001 respectively).

Conclusion

The central retinal and choroidal thickness were thinner in vitamin C deficiency group compared with normal individuals. These findings suggested that vitamin C deficiency might play an important role in retinal and choroidal diseases.
Fundamental motor skills of kindergarten children in different environments and ethnic groups in Northwest China
Authors: Xia Xiaohui , Chao Liang , Nan Chen , Yin Xuejuan , Zheng Huifang , Zhang Sheping
Published: July 2022

Background

The status of children’s early motor skills play an important role during childhood and across lifetime. This study described FMS proficiency among boys (n = 189) and girls (n = 179) kindergarten children from 3 to 6 years old (4.4 s 0.7, mean ± SD) in northwest China. The differences in FMS proficiency of boys and girls from different environments, ethnic groups were analyzed respectively.

Methods

TGMD-3 was used to assess FMS. FMS mastery level was defined according to the correct performance of all criteria over two trials. The correlation between BMI and FMS and the interaction of environmental and ethnic on FMS were analyzed. The general linear model was used to evaluate the differences of boys and girls among environment groups (urban/suburban/county), and ethnic groups (Han/Hui/Tibetan) on the FMS subsets respectively.

Results

FMS proficiency was assessed in 368 3- to 6-year-old children (n = 156 urban, n = 101 suburban, n = 111 county)/(n = 208 Han, n = 107 Hui, n = 53 Tibetan). Overall, the highest skill performance was the run, with 86% achieving mastery level, and the poorest performance was the FH strike, at only 19%. Correlation between BMI and FMS is minimal. According to TGMD-3 scores, there was no significant difference between boys and girls in total FMS (p = 0.38). In terms of locomotor skills, boys performed better than girls in the hop, skip and slide (p < 0.05). County children performed significantly difference than urban and suburban children. Some skills performed less proficiently, (boys in 6 of 13 skills: run, HJ, slide, TH strike, FH strike and kick; girls in 4 of 13 skills: run, slide, TH strike and kick) and some skills performed more proficiently (boys in dribble; girls in hop and dribble). Tibetan children performed significantly difference than Han and Hui children. Some skills performed less proficiently, (boys in 6 of 13 skills: run, HJ, slide, TH strike, FH strike and kick; girls in TH strike) and some skills performed more proficiently (boys and girls were all in dribble).

Conclusion

Children in northwest China showed certain characteristics in FMS, the county/Tibetan boys and girls performed poorer than others in ability to execute particular process characteristics of some skills and performed more outstanding in other skills. It suggests that a certain group population may need specific focus on interventions to improve their FMS level.
Can preoperative ureteral stents reduce the incidence of ureteral stricture after radiotherapy in patients with cervical cancer?
Authors: Liu Liang , Yu Chunhong , Sun Fuzhen , Yang Tao , Wei Dong , Wang Gang , Li Shoubin , Liu Junjiang
Published: July 2022

Objective

To determine the impact of preoperative stent placement on postradiotherapy stricture rate in patients with cervical cancer after radical resection.

Methods

This study was a retrospective analysis of data collected from 55 cervical cancer patients treated with radiotherapy between June 2016 and June 2020. Patients were divided into the stent and control groups. After 3 months, the stricture rate and the complications related to stent placement between the two groups were compared.

Results

There were 12 (46.2%) and 10 (34.5%) cases of ureteral stricture in the stent (n = 26) and control (n = 29) groups, respectively, three months after the end of radiotherapy. The incidence rates of ureter stricture in the two groups were not significantly different (P = 0.378). Moreover, there were 20 units (38.5%) and 15 units (25.9%) ureteral strictures in the stent and control groups, respectively. No significant difference in the incidence rates of ureteral strictures was found between the two groups (P = 0.157). There were 13 (50.0%) and 10 (34.5%) cases of ureteral stricture in the stent (n = 26) and control (n = 29) groups, respectively, six months after the end of the radiotherapy. The incidence rates of ureter stricture in the two groups were not significantly different (P = 0.244). Moreover, there were 21 units (40.4%) and 15 units (25.9%) ureteral strictures in the stent and control groups, respectively. No significant difference in the incidence rates of ureteral strictures was found between the two groups (P = 0.105). Complications related to stent placement such as urinary tract infections and bladder irritation were statistically significant (P = 0.006 and P = 0.036) between the two groups; while the other complications were not significantly different (P = 0.070, P = 0.092 and P = 0.586).

Conclusions

Ureteral stents may not reduce the incidence of ureteral stricture after radiotherapy in patients with cervical cancer. The stent needs to be replaced regularly, and the complications related to stent placement may occur at any time. Thus, preoperative stent placement should be cautious for the clinical management of cervical cancer patients treated with postoperative radiotherapy.
Efficient production of a cyclic dipeptide (cyclo-TA) using heterologous expression system of filamentous fungus Aspergillus oryzae
Authors: Qi Jianzhao , Han Haiyan , Sui Dan , Tan Shengnan , Liu Changli , Wang Pengchao , Xie Chunliang , Xia Xuekui , Gao Jin-ming , Liu Chengwei
Published: July 2022

Background

Cyclic dipeptides are an important class of natural products owing to their structural diversity and biological activities. In fungi, the cyclo-ring system is formed through the condensation of two α-amino acids via non-ribosomal peptide synthetase (NRPS). However, there are few investigations on the functional identification of this enzyme. Additionally, information on how to increase the production of cyclic dipeptide molecules is relatively scarce.

Results

We isolated the Eurotium cristatum NWAFU-1 fungus from Jing-Wei Fu brick tea, whose fermentation metabolites contain echinulin-related cyclic dipeptide molecules. We cloned the cirC gene, encoding an NRPS, from E. Cristatum NWAFU-1 and transferred it into the heterologous host Aspergillus oryzae. This transformant produced a novel metabolite possessing an l-tryptophan-l-alanine cyclic dipeptide backbone (Cyclo-TA). Based on the results of heterologous expression and microsomal catalysis, CriC is the first NRPS characterized in fungi that catalyzes the formation of a cyclic dipeptide from l-tryptophan and l-alanine. After substrate feeding, the final yield reached 34 mg/L. In this study, we have characterized a novel NRPS and developed a new method for cyclic dipeptide production.

Conclusions

In this study we successfully expressed the E. Cristatum NWAFU-1 criC gene in A. oryzae to efficiently produce cyclic dipeptide compounds. Our findings indicate that the A. oryzae heterologous expression system constitutes an efficient method for the biosynthesis of fungal Cyclic dipeptides.
Adherence to a healthy and potentially sustainable Nordic diet is associated with child development in The Norwegian Mother, Father and Child Cohort Study (MoBa)
Authors: Vejrup Kristine , Agnihotri Neha , Bere Elling , Schjølberg Synnve , LeBlanc Marissa , Hillesund Elisabet Rudjord , Øverby Nina Cecilie
Published: July 2022

Background

The rapid neurodevelopment that occurs during the first years of life hinges on adequate nutrition throughout fetal life and early childhood. Therefore, adhering to a dietary pattern based on healthy foods during pregnancy and the first years of life may be beneficial for future development. The aim of this paper was to investigate the relationship between adherence to a healthy and potentially sustainable Nordic diet during pregnancy and in early childhood and child development.

Methods

This study is based on the Norwegian Mother, Father and Child Cohort Study (MoBa) and uses data from the Medical Birth Registry of Norway (MBRN). In 83,800 mother-child pairs, maternal pregnancy diet and child diet at 6 months, 18 months and 3 years were scored according to adherence to the New Nordic Diet (NND). NND scores were calculated both as a total score and categorized into low, medium, or high adherence. Child communication and motor development skills were reported by parents at 6 months, 18 months, 3 and 5 years, using short forms of the Ages and Stages Questionnaire and the Child Development Inventory. Associations of NND adherence with child development were estimated with linear and logistic regression in crude and adjusted models.

Results

When examining the NND and child developmental scores as percentages of the total scores, we found positive associations between the NND scores (both maternal pregnancy diet and child diet) and higher scoring on child development (adjusted$$\hat{\beta}$$β^s [95% confidence intervals] ranging from 0.007 [0.004, 0.009] to 0.045 [0.040, 0.050]). We further found that low and medium adherence to NND were associated with higher odds of later emerging developmental skills compared to high NND adherence at nearly all measured timepoints (odds ratios [95% CI] ranging from significant values 1.15 [1.03–1.29] to 1.79 [1.55, 2.06] in adjusted analyses).

Conclusions

Our findings support that adherence to a healthy and potentially sustainable diet early in life is important for child development every step of the way from pregnancy until age 5 years.
Effects of macrophages on the proliferation and cardiac differentiation of human induced pluripotent stem cells
Authors: Long Canling , Guo Rui , Han Ruijuan , Li Kang , Wan Yanbing , Xu Jiqing , Gong Xiaoyu , Zhao Yanqiu , Yao Xinhuang , Liu Jia
Published: July 2022

Background

Macrophage phenotypes switch from proinflammatory (M1) to anti-inflammatory (M2) following myocardial injury. Implanted stem cells (e.g., induced pluripotent stem cells (iPSCs)) for cardiomyogenesis will inevitably contact the inflammatory environment at the myocardial infarction site. To understand how the macrophages affect the behavior of iPSCs, therefore, improve the therapeutic efficacy, we generated three macrophage subtypes and assessed their effects on the proliferation, cardiac differentiation, and maturation of iPSCs.

Methods

M0, M1, and M2 macrophages were polarized using cytokines, and their properties were confirmed by the expression of specific markers using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence. The effects of macrophages on iPSCs were studied using Transwell co-culture models. The proliferative ability of iPSCs was investigated by cell counting and CCK-8 assays. The cardiac differentiation ability of iPSCs was determined by the cardiomyocyte (CM) yield. The maturation of CM was analyzed by the expression of cardiac-specific genes using RT-qPCR, the sarcomere organization using immunofluorescence, and the mitochondrial function using oxidative respiration analysis.

Results

The data showed that the co-culture of iPSCs with M0, M1, or M2 macrophages significantly decreased iPSCs’ proliferative ability. M2 macrophages did not affect the CM yield during the cardiac differentiation of iPSCs. Still, they promoted the maturation of CM by improving sarcomeric structures, increasing contractile- and ion transport-associated gene expression, and enhancing mitochondrial respiration. M0 macrophages did not significantly affect the cardiomyogenesis ability of iPSCs during co-culture. In contrast, co-culture with M1 macrophages significantly reduced the cardiac differentiation and maturation of iPSCs.

Conclusions

M1- or M2-polarized macrophages play critical roles in the proliferation, cardiac differentiation, and maturation of iPSCs, providing knowledge to improve the outcomes of stem cell regeneration therapy.
Phagocytosis converts infiltrated monocytes to microglia-like phenotype in experimental brain ischemia
Authors: Ju Hyunwoo , Park Keun Woo , Kim Il-doo , Cave John W. , Cho Sunghee
Published: July 2022

Background

Monocyte-derived macrophages (MDMs) and microglia elicit neural inflammation and clear debris for subsequent tissue repair and remodeling. The role of infiltrating MDMs in the injured brain, however, has been controversial due to overlapping antigen expression with microglia. In this study, we define the origin and function of MDMs in cerebral ischemia.

Methods

Using adoptive transfer of GFP+ splenocytes into adult asplenic mice subjected to transient middle cerebral artery occlusion, we compared the role of CD11b+/CD45+/NK1.1−/Ly6G− MDMs and microglia in the ischemic brain. The phagocytic activities of MDMs and microglia were measured by the uptake of fluorescent beads both in vivo with mice infused with GFP+ splenocytes and ex vivo with cultures of isolated brain immune cells.

Results

Stroke induced an infiltration of MDMs [GFP+] into the ipsilateral hemisphere at acute (3 days) and sub-acute phases (7 days) of post-stroke. At 7 days, the infiltrating MDMs contained both CD45High and CD45Low subsets. The CD45High MDMs in the injured hemisphere exhibited a significantly higher proliferation capacity (Ki-67 expression levels) as well as higher expression levels of CD11c when compared to CD45Low MDMs. The CD45High and CD45Low MDM subsets in the injured hemisphere were approximately equal populations, indicating that CD45High MDMs infiltrating the ischemic brain changes their phenotype to CD45Low microglia-like phenotype. Studies with fluorescent beads reveal high levels of MDM phagocytic activity in the post-stroke brain, but this phagocytic activity was exclusive to post-ischemic brain tissue and was not detected in circulating monocytes. By contrast, CD45Low microglia-like cells had low levels of phagocytic activity when compared to CD45High cells. Both in vivo and ex vivo studies also show that the phagocytic activity in CD45High MDMs is associated with an increase in the CD45Low/CD45High ratio, indicating that phagocytosis promotes MDM phenotype conversion.

Conclusions

This study demonstrates that MDMs are the predominant phagocytes in the post-ischemic brain, with the CD45High subset having the highest phagocytic activity levels. Upon phagocytosis, CD45High MDMs in the post-ischemic brain adopt a CD45Low phenotype that is microglia-like. Together, these studies reveal key roles for MDMs and their phagocytic function in tissue repair and remodeling following cerebral ischemia.
Subacute and chronic proteomic and phosphoproteomic analyses of a mouse model of traumatic brain injury at two timepoints and comparison with chronic traumatic encephalopathy in human samples
Authors: Morin Alexander , Davis Roderick , Darcey Teresa , Mullan Michael , Mouzon Benoit , Crawford Fiona
Published: July 2022

Repetitive mild traumatic brain injury (r-mTBI) is the most widespread type of brain trauma worldwide. The cumulative injury effect triggers long-lasting pathological and molecular changes that may increase risk of chronic neurodegenerative diseases. R-mTBI is also characterized by changes in the brain proteome, where the majority of molecules altered early post-TBI are different from those altered at more chronic phases. This differentiation may contribute to the heterogeneity of available data on potential therapeutic targets and may present an obstacle in developing effective treatments. Here, we aimed to characterize a proteome profile of r-mTBI in a mouse model at two time points – 3 and 24 weeks post last TBI, as this may be a more relevant therapeutic window for individuals suffering negative consequences of r-mTBI. We identified a great number of proteins and phosphoproteins that remain continuously dysregulated from 3 to 24 weeks. These proteins may serve as effective therapeutic targets for sub-acute and chronic stages of post r-mTBI. We also compared canonical pathway activation associated with either total proteins or phosphoproteins and revealed that they both are upregulated at 24 weeks. However, at 3 weeks post-TBI, only pathways associated with total proteins are upregulated, while pathways driven by phosphoproteins are downregulated. Finally, to assess the translatability of our data, we compared proteomic changes in our mouse model with those reported in autopsied human samples of Chronic Traumatic Encephalopathy (CTE) patients compared to controls. We observed 39 common proteins that were upregulated in both species and 24 common pathways associated with these proteins. These findings support the translational relevance of our mouse model of r-mTBI for successful identification and translation of therapeutic targets.