bluuebunny/crossref_metadata_2025 huggingface dataset:
Zinc transporter ZIP13 is involved in myogenic differentiation: establishment of Ehlers– Danlos syndrome spondylodysplastic type 3 induced pluripotent stem cells
Authors: Shoji Masaki , Ohashi Takuto , Nagase Saki , Ichihashi Kenta , Takagishi Teruhisa , Nagata Yuji , Nomura Yuki , Hara Takafumi , Yoshigai Emi , Fukunaka Ayako , Fujitani Yoshio , Sakurai Hidetoshi , Santos Heloísa G. dos , Fukada Toshiyuki , Kuzuhara Takashi
Published: December 2022

Ehlers–Danlos syndrome spondylodysplastic type 3 (EDSSPD3, OMIM 612350) is an inherited recessive connective tissues disease caused by loss of function of SLC39A13/ZIP13, a zinc transporter belonging to the Slc39a/ZIP family. Patients with EDSSPD3 suffer from impaired development of bone and connective tissues, and muscular hypotonia, or myopathy. However, whether ZIP13 participates in the early differentiation process of these cell types remains unclear. In this study, we investigated the role of ZIP13 in myogenic differentiation using murine myoblast cell line (C2C12) as well as human patient-derived induced pluripotent stem cells (iPSCs). We found that ZIP13 expression was upregulated by myogenic stimulation in C2C12 cells, and its knockdown disrupted myotubular differentiation. Myocytes differentiated from iPSCs of patients with EDSSPD3 (EDSSPD3-iPSCs) exhibited incomplete myogenic differentiation. Moreover, the phenotypic abnormalities of EDSSPD3-iPSC-derived myocytes were corrected by genomic editing of the pathogenic ZIP13 mutation, suggesting the indispensable role of ZIP13 in myogenic differentiation. These results clearly indicate that ZIP13 is required for proper myogenic differentiation and that the study of EDSSPD3-iPSCs may help shed light on the molecular basis underlying various clinical features caused by the loss of ZIP13.
Electronic Optimization and Modification of Efficient Ir Clusters Embedded onto Ni-Mo-P for Electrocatalytic Oxygen Evolution Reaction
Authors: Wei Hehe , Li Jing , Du Peng , Zhang Longtao , Su Zixiang , Li Hui , Lei Ming , Huang Kai , Gong Xueqing
Published: December 2022

The implementation of low-cost and efficient electrocatalysts for water oxidation is crucial for the development of industrial water electrolysis, however, they often suffer from inferior activity or poor stability. Herein, we demonstrated 0.7-nm iridium clusters embedded onto Ni-Mo-P (Ir-NMP) that exhibited an ultralow overpotential of 290 mV vs reversible hydrogen electrode (RHE) to reach the current density of 50 mA cm− 2 in 1 M KOH, together with low Tafel slop of 35.3 mV dec− 1, high mass activity of 2604 A gIr−1 and excellent catalytic stability with almost complete retention of activity within more than 30 hours. According to characterizations and analysis, the interlaced crystalline and amorphous structure of Ni-Mo-P made homogeneous embedment of ultrathin Ir clusters onto NMP substrate, and the introduction of Ir clusters enabled the optimization of electronic structure for Ni and Mo species in NMP, which were available to highly efficient and durable Ir-NMP electrocatalyst for OER.
Quasi-seamless stitching for large-area micropatterned surfaces enabled by Fourier spectral analysis of moiré patterns
Authors: Kim Woo Young , Seo Bo Wook , Lee Sang Hoon , Lee Tae Gyung , Kwon Sin , Chang Won Seok , Nam Sang , Fang Nicholas , Kim Seok , Cho Young Tae
Published: December 2022

The main challenge in preparing a flexible mold stamp using roll-to-roll nanoimprint lithography is to simultaneously increase the imprintable area with a minimized perceptible seam. However, the current methods for stitching multiple small molds to fabricate large-area molds and functional surfaces typically rely on the alignment mark, which inevitably produces a clear alignment mark and stitched seam. In this study, a self-pattern alignment (SPA) method inspired by moiré engineering is proposed to fabricate scalable functional surfaces and flexible imprint molds with quasi-seamless and alignment mark-free patterning, which is based on the Fourier spectral analysis of moiré patterns formed by superposed identical patterns. By harnessing the rotational invariance property in the Fourier transform, SPA was confirmed to be a simple and efficient method for extracting the rotational and translational offsets in overlapped periodic or nonperiodic patterns with a minimized stitched region, thereby allowing for the large-area and quasi-seamless fabrication of imprinting molds and functional surfaces, such as liquid-repellent film and micro-optical sheets, that surpass the conventional alignment and stitching limits and potentially expand their application in producing large-area metasurfaces.
A Conceptual framework of Cognitive-Affective Theory of Mind: Towards a Precision Identification of Mental Disorders
Authors: Zhou Peng , Ma Huimin , Zou Bochao , Zhang Xiaowen , Zhao Shuyan , Lin Yuxin , Wang Yidong , Feng Lei , Wang Gang
Published: December 2022

To explore the minds of others, which is traditionally referred to as Theory of Mind (ToM), is perhaps the most fundamental ability of humans as social beings. Impairments in ToM could lead to difficulties or even deficits in social interaction. The present study focused on two core components of ToM, the ability to infer others’ beliefs and the ability to infer others’ emotions, which we refer to as cognitive and affective ToM respectively. Charting both typical and atypical trajectories underlying the cognitive-affective ToM promises to shed light on the precision identification of mental disorders, such as depressive disorders (DD) and autism spectrum disorder (ASD). However, most prior studies failed to capture the underlying processes involved in the cognitive-affective ToM in a fine-grained manner. To address this problem, we propose an innovative conceptual framework, referred to as visual theory of mind (v-ToM), by constructing visual scenes with emotional and cognitive meanings and by depicting explicitly a four-stage process of how humans make inferences about the beliefs and emotions of others. Through recording individuals’ eye movements while looking at the visual scenes, our model enables us to accurately measure each stage involved in the computation of cognitive-affective ToM, thereby allowing us to infer about potential difficulties that might occur in each stage. Our model is based on a large sample size (n > 700) and a novel audio-visual paradigm using visual scenes containing cognitive-emotional meanings. The obtained differential features among normal people, DD and ASD individuals overcome the subjectivity of conventional questionnaire-based assessment, and therefore could serve as valuable references for mental health applications based on AI-aided digital medicine.
Extraction of normalized Digital Surface Model (nDSM) from LiDAR Data in Forest Inventory Mapping
Authors: Faisal Abdullah-Al- , Afroz Farzana , Kafy Abdulla Al
Published: December 2022

The retrieval of Light Detection and Ranging (LiDAR) data is a complex procedure that necessitates extensive processing in order to develop terrain and surface models and forest structure applications. The gradual acquisition of LiDAR information is required to create Digital Elevation Models (DEM) and Digital Surface Models (DSM). The purpose of the study was to generate topographic DEM and normalized DSM (nDSM) data from LiDAR point cloud and to outline the canopy height extraction procedure in the New Forest region of the United Kingdom. Later, under 21 random enclosures, a demonstration of how the nDSM can be used in forest inventory mapping was discussed. The results show that, of the various interpolation techniques used to generate DEM, IDW had the lowest RMSE value of 0.382. The Digital Terrain Model (DTM) was created using two neighborhood settings (3×3) and (30×30), with the last one showing higher accuracy. In the comparison of different interpolation techniques, Inverse Distance Weighting (IDW) was found to have the lowest RMSE value of 0.382. Finally, within the enclosures, the percentage of no trees (mostly shrubs), canopy height ranged 2-10m, 10-15m, and > 15 was mapped. Each enclosure with 40% of its area covered by trees taller than 15 m was assumed to be harvestable. The study demonstrated detailed algorithm-based LiDAR data extraction and processing, which can be used to explore and forecast terrestrial ecosystems with advanced longitudinal orientation potentialities.
Soil CO2, CH4 and N2O fluxes in urban forests, treed and open lawns in Angers, France
Authors: Künnemann Tom , Cannavo Patrice , Guérin Vincent , Guénon René
Published: December 2022

Urban green spaces (UGSs) are mostly represented by lawns and forests. These UGSs can store carbon in soil and above-ground biomass, potentially modulated by management intensity and vegetation cover (shading, rainfall intercept, litterfall, …). Trees in lawns can create a local microclimate modifying soil biogeochemical cycles affecting in turn greenhouse gas (GHG) emissions. The objective of this study was to assess the effects of trees on microclimate (temperature and moisture) influencing GHG in contrasted UGS types. We monthly monitored (from March to November 2021) and compared soil CO2, CH4 and N2O fluxes simultaneously with surface temperature and moisture in treed lawns, open lawns and urban forests. Lawns included 4 different management intensities including mowing, irrigation and fertilization practices. Temperature was the best predictor of soil respiration in all UGS types studied and was the highest in open lawns. We showed that moisture reflected by the water filled pore space (WFPS) significantly added on variation explanation. The shading of trees strongly decreased soil respiration in treed lawns while soil properties were similar indicating a straightforward effect of lowering temperature. On the contrary, forests deeply changed soil properties as well as decreased soil temperature resulting in the lowest rates of soil respiration. Urban forests are a sink for CH4 throughout the year. Lawns were weak to mitigate CH4 and a source of CH4 in irrigated parks where WFPS overpassed 75%. N2O fluxes were weak probably reflecting the transition already made from mineral to organic fertilization limiting N availability.
Telephone outreach by volunteer navigators: a theory-based evaluation of an intervention to improve access to appropriate primary care
Authors: Haggerty Jeannie , Smithman Mélanie-Ann , Beaulieu Christine , Breton Mylaine , Dionne Émilie , Lewis Virginia
Published: December 2022

Background: Pilot interventions were implemented in five different regions within a Canada-Australia participatory research programme to improve access to primary healthcare for vulnerable populations. In Québec, Canada, a telephone outreach program used volunteer patient navigators to help unattached persons from deprived neighbourhoods attach successfully to a family doctor newly-assigned to them from a centralized waiting list. According to our theory-based program logic model we evaluated the extent to which the volunteer navigator outreach helped patients reach and engage with their newly-assigned primary care team, have a positive healthcare experience, develop an enduring doctor-patient relationship, and reduce forgone care and emergency room use.
Method: For the mixed-method evaluation, indicators were developed for all domains in the logic model and measured in a telephone-administered survey at baseline and three months later to determine if there was a significant difference. Semi-structured with a subsample of 13 survey respondents explored the mechanisms and nuances of intended effects.
Results: Five active volunteers provided the service to 108 persons, of whom 60 agreed to participate in the evaluation. All surveyed participants attended the first visit, where 90% attached successfully to the new doctor. Indicators of abilities to access healthcare increased statistically significantly as did ability to explain health needs to professionals. The telephone outreach predisposed patient to have a positive first visit and have trust in their new care team, establishing a basis for an enduring relationship. Access difficulties, foregone care and use of hospital emergency rooms decreased dramatically after patients attached to their new doctors.
Conclusions: As per the logic model, telephone outreach by volunteer navigators significantly increased patients’ abilities to seek, reach and engage with care and helped them attach successfully to newly-assigned family doctors. This light-touch intervention led to achievement of the intended policy goals for the centralized waiting list to increase population access to appropriate primary care and reducing forgone care and use of emergency rooms.
Leakage reduction of Water Distribution Networks with Clustering
Authors: Huzsvár Tamás , Wéber Richárd , Szabó Marcell , Hős Csaba
Published: December 2022

Nowadays, the rapid growth of operational costs and human resource shortages increase the importance of the leakage reduction, and the pressure management. This paper presents an optimisation procedure placing pressure-reducing valves (PRVs) to minimise the water losses of real-life water distribution networks (WDNs). The number and placement of the valves are optimised from clustering perspective with the Leiden algorithm. The optimal settings of the valves are determined with a differential evolution algorithm, where a compound fitness function is applied. On the one hand, it minimises the leakages, on the other hand, evades the pressure-dependent consumer outages, i.e. it keeps the pressure above the desired. Seven real-life water distribution networks were analysed from the region of Western Hungary using the techniques. Moreover, three of them are analysed in details. The robustness of the achieved leakage reduction with the optimised PRV placement is analysed to the uncertainty of the leakage exponent.
Advancing positive social determinants of health through collective impact
Authors: McCrae Julie S. , Spain Angeline K.
Published: December 2022

Background
Communities across the U.S. and globally confront the challenge of transforming negative social determinants of health (SDOH) into positive ones. To address this complex social problem, collective impact (CI) has promise but has been critiqued for insufficiently challenging structural inequities. Research applying CI to SDOH is limited. This mixed-methods study examined early adoption of CI in the 100% New Mexico initiative that aims to address SDOH population-wide in a state with strong cultural identity and assets but also persistent socio-economic inequality. |
Methods. A web-based survey, interviews and focus groups were conducted with initiative participants in June and July 2021. Survey participants rated agreement with six items assessing CI foundation from the Collective Impact Community Assessment Scale. Interviews and focus groups centered on motivation to engage, progress in model components, CI core conditions, and contextual factors influencing experiences. Surveys were analyzed using descriptive means. Qualitative data were analyzed using thematic analysis, an inductive approach, stratified analyses, and co-interpretation. Results. Fifty-eight participants completed the survey, and 21 participated in interviews (n = 12) and focus groups (n = 9). Survey means were highest related to initiative commitment, and lower regarding shared ownership, having multiple perspectives and voices, and adequate resources. Qualitative results indicated the framework’s cross-sector emphasis helped motivate participation. Participants embraced the focus on leveraging community assets that characterizes CI and the current framework. Counties used unique engagement and visibility strategies including mural projects and book clubs. Participants reported communication challenges across sector teams which influenced feelings of accountability and ownership. Participants did not report challenges lacking relevant, available, and timely data or tension between funder-driven and community-driven desired outcomes, contrasting previous CI research.
Conclusion. Multiple foundational conditions of CI were supported in 100% New Mexico, including evidence for support of the common agenda addressing SDOH, shared measurement framework, and mutually reinforcing activities. Results suggest that efforts to launch CI to address SDOH, which are by nature multi-sector, should include robust communication support for local teams. The use of community-administered surveys to identify gaps in SDOH resource access contributed to ownership and collective efficacy that may portend sustainability; however, relying on volunteers threatens sustainability.
Partial trisomy 21 with or without highly restricted-Down syndrome critical region (HR- DSCR). Report of two new cases and reanalysis of the genotype-phenotype association
Authors: Pelleri Maria Chiara , Locatelli Chiara , Mattina Teresa , Bonaglia Maria Clara , Piazza Francesca , Magini Pamela , Antonaros Francesca , Ramacieri Giuseppe , Vione Beatrice , Vitale Lorenza , Seri Marco , Strippoli Pierluigi , Cocchi Guido , Piovesan Allison , Caracausi Maria
Published: December 2022

Background
Down syndrome (DS) is caused by the presence of an extra copy of full or partial human chromosome 21 (Hsa21). Partial (segmental) trisomy 21 (PT21) is the duplication of only a delimited region of Hsa21 and can be associated or not to DS: the study of PT21 cases is an invaluable model for addressing genotype-phenotype correlation in DS. Previous works reported systematic reanalyses of 132 subjects with PT21 and allowed the identification of a 34-kb highly restricted DS critical region (HR-DSCR) as the minimal region whose duplication is shared by all PT21 subjects diagnosed with DS.
Methods
We report clinical data and cytogenetic analysis of two children with PT21, one with DS and the other without DS. Moreover, we performed a systematic bibliographic search for any new PT21 report.
Results
Clinical and cytogenetic analyses of the two PT21 children have been reported: in Case 1 the duplication involves the whole long arm of Hsa21, except for the last 2.7 Mb, which are deleted as a consequence of an isodicentric 21: the HR-DSCR is within the duplicated regions and the child is diagnosed with DS. In Case 2 the duplication involves 7.1 Mb of distal 21q22, with a deletion of 2.1 Mb of proximal 20p, as a consequence of an unbalanced translocation: the HR-DSCR is not duplicated and the child presents with psychomotor development delay but no clinical signs of DS. Furthermore, two PT21 reports recently published (named Case 3 and 4) have been discussed: Case 3 has DS diagnosis, nearly full trisomy for Hsa21 and a monosomy for the 21q22.3 region. Case 4 is a baby without DS and a 0.56-Mb duplication of 21q22.3. Genotype-phenotype correlation confirmed the presence of three copies of the HR-DSCR in all DS subjects and two copies in all non-DS individuals.
Conclusions
The results presented here are fully consistent with the hypothesis that the HR-DSCR is critically associated with DS diagnosis. No exception to this pathogenetic model was found. Further studies are needed to detect genetic determinants likely located in the HR-DSCR and possibly responsible for core DS features, in particular intellectual disability.
Molecular Characterization of Metal Tolerant Bacteria Isolated from Contaminated Soil
Authors: Mairaj Asma , Saeed Muhammad Faran , Sarwar Qurat-ul-Ain
Published: December 2022

Soil pollution with toxic metals is an alarming threat to the agricultural sector as these are non-biodegradable. The problem is getting worse in developing countries like Pakistan. Several methods remove the toxic metals from the environment including physical, chemical, and biological processes. Physical and chemical methods are not only expensive but also produce secondary by-products that may damage the ecosystem. Bioremediation is a cost-effective method that reduced environmental pollution by using biodegradation processes. Therefore, this study was carried out for the isolation and characterization of metal tolerant bacteria from wastewater irrigated soil in Multan. Contaminated soil samples were collected from wastewater irrigated soil, and were inoculated on Nutrient agar poured with hexavalent chromium. Positive cultures were freshed on Nutrient agar on basis of colony morphology, and pure colonies were subjected to varying concentrations of metal to determine the Minimum Inhibitory Concentration (MIC). The broth dilution method was used for the determination of minimum inhibitory concentration. Out of four soil specimens, a total of six bacteria belonging to the two different species were isolated and identified on basis of gram staining and biochemical characterization. Differentiation of gram-positive rods on basis of colony morphology and biochemical tests were identified as Bacillus subtilis (n = 4) and Bacillus cereus (n = 2). Gene amplification through specific primers for 16S rDNA was performed according to the protocol. For further evaluation and sequencing, isolates have been sent to Macrogen (Korea).
The effects of high plasma levels of Aβ 1-42 on mononuclear macrophage in mouse models of Alzheimer’s disease
Authors: Li Chunrong , Liu Kangding , Zhu Jie , Zhu Feiqi
Published: December 2022

Microglia plays a crucial role in the pathogenesis of Alzheimer’s disease (AD). Plasma Aβ1−42 levels significantly increased 15 years before the onset of dominantly inherited AD. The effects of high plasma levels of Aβ1−42 on mononuclear macrophage, the peripheral counterparts of microglia, remains unclear. Herein, we used a parabiosis model to investigate the effects of plasma Aβ1−42 on peripheral mononuclear macrophage and the possible mechanism. We found the proportions of pro-inflammatory macrophages in peritoneal cavity, myeloid derived suppressor cells (MDSCs) in spleen, granulocyte-monocyte progenitors (GMP) in bone marrow, and the plasma levels of interleukin-6 (IL-6) were significantly decreased after 4 months sustaining stimulation with high plasma levels of Aβ1−42. The proportions of pro-inflammatory macrophages, MDSCs, GMPs, the plasma levels of IL-6 and TNF-α, as well as the numbers of bone marrow-derived macrophages (BMDMs) in mice brain were increased after 8 months sustaining stimulation with high plasma levels of Aβ1−42. In addition, the proportions of monocytes in spleen and the proliferation of bone marrow cells (BMCs) were increased, however, the phagocytic function of macrophages had no obviously alteration after high plasma levels of Aβ1−42 sustaining stimulation. These results demonstrated that high plasma levels of Aβ1−42 had a biphasic regulating effects on the amounts of pro-inflammatory macrophages, MDSCs, and bone marrow myeloid progenitor cells, as well as the secretory function of macrophage. Herein, we suggest that the alterations of the amounts and proliferations of myeloid progenitor cells in bone marrow may be involved in the regulation of peripheral mononuclear macrophages and MDSCs, intervening the effects of plasma Aβ1−42 on monocytes/macrophages might offer a new therapeutic approach to AD.
A Case of Atypical Rabies with Adams-Stokes Syndrome ： a case report
Authors: Song Yuwei , Wang Miaomiao , Jiang Hongyu , Wang Yujie , Zhu Lin , Liu Ning , Zhu Bin
Published: December 2022

Background
Rabies is an infectious disease of animal origin caused by Rabies lyssavirus, commonly known as the Rabies virus (RABV), and leads to acute encephalitis or meningoencephalitis. However, no single test is sufficient for a precise diagnosis. Moreover, the diagnosis of rabies is also difficult for individuals with atypical symptoms or multizonal failures, which lead to a higher rate of clinical misdiagnosis. Therefore, there is an urgent need to develop a sensitive method to diagnose such patients.
Case presentation:
We report a case of atypical rabies with Adams-Stokes syndrome. A 70-year-old male was admitted to hospital with vomiting for five consecutive days and recurrent loss of consciousness for three days. Electrocardiography monitoring showed frequent sinus arrest and third-degree atrioventricular block, suggesting Adams-Stokes syndrome. Percutaneous external noninvasive pacing therapy was immediately administered. On the third day of admission, the patient tested positive for RABV in bronchoalveolar lavage fluid (BALF) using metagenomic next-generation sequencing (mNGS). The patient then developed psychiatric symptoms such as convulsions and eventually deteriorated to multiple organ failure on the fifth day of admission. The patient’s disease developed rapidly after showing typical symptoms of rabies and the patient died on the same day that he voluntarily discharged.
Conclusion
We demonstrated that mNGS could be used as a complementary tool for diagnosing rabies, especially for individuals with unclear exposure histories or atypical symptoms.
The secondary metabolites of Bacillus subtilis strain Z15 Induce Apoptosis in Hepatocellular Carcinoma Cells
Authors: Aimaier Reyihanguli , Li Haoran , Cao Wenzhi , Cao Xiyuan , Zhang Hui , You Jia , Zhao Jingjing , Zhang Qi , Yin Li , Mei Qinshuang , Zhang Xiaorong , Wang Weiquan , Zhao Huixin , Li Jinyu , Zhao Heping
Published: December 2022

The lipopeptides produced by Bacillus subtilis have anti-cancer potential. We had previously identified a secondary metabolite of B. subtilis strain Z15 (BS-Z15), which has an operon that regulates lipopeptide synthesis, and also demonstrated that the fermentation products of this strain exerted antioxidant and pro-immune effects. The purpose of this study was to investigate in vitro and in vivo the anticancer effects of BS-Z15 secondary metabolites (BS-Z15 SMs) on hepatocellular carcinoma (HCC) cells. BS-Z15 SMs significantly inhibited H22 cell-derived murine xenograft tumor growth without any systemic toxicity. In addition, BS-Z15 SMs decreased the viability of H22 cells and BEL-7404 cells in vitro with respective IC50 values of 33.83µg/mL and 27.26 µg/mL. Consistent with this, BS-Z15 SMs induced apoptosis and G0/G1 phase arrest in the BEL-7404 cells, and the mitochondrial membrane potential was also significantly reduced in a dose-dependent manner. Mechanistically, BS-Z15 SMs upregulated the pro-apoptotic p53, Bax, cytochrome C and cleaved-caspase-3/9 proteins, and downregulated the anti-apoptotic Bcl-2.These findings suggest that the induction of apoptosis in HCC cells by BS-Z15 SMs may be related to the mitochondrial pathway. Thus, the secondary metabolites of B. subtilis strain Z15 are promising to become new anti-cancer drugs for the clinical treatment of liver cancer.
The use of an individual-based FDG-PET volume of interest approach in mild cognitive impairment: a multi-modality longitudinal follow-up study
Authors: Huang Shu-Hua , Hsiao Wen-Chiu , Huang Chi-Wei , Chang Hsin-I , Ma Mi-Chia , Hsu Shih-Wei , Lee Chen-Chang , Chen Hong-Jie , Lin Ching-Heng , Chang Chiung-Chih
Published: December 2022

Background:
Based on a longitudinal cohort design, the aim of this study was to investigate whether individual-based 18F fluorodeoxyglucose positron emission tomography (18F-FDG-PET) regional signals can predict dementia conversion in patients with mild cognitive impairment (MCI).
Methods:
We included 44 MCI converters (MCI-C), 38 non-converters (MCI-NC), 42 patients with amyloid-positive Alzheimer disease, and 40 cognitively normal controls. Data from annual cognitive measurements, 3D T1 magnetic resonance imaging (MRI) scans, and 18F-FDG-PET scans were used for outcome analysis. An individual-based FDG-PET approach was defined using seven volumes of interest (VOIs), Z transformed using a normal FDG-PET template. Hypometabolism was defined as a Z score < -2 of regional standard uptake value ratio. For the longitudinal cognitive test scores, generalized estimating equations were used. A linear mixed effect model was used to compare the time effect of cortical hypometabolism and cortical thickness degeneration.
Results
The clinical follow-up period was 6.6 ± 3.8 years (range 3.1 to 16.0 years). The cognitive decline trends could differentiate MCI-C from MCI-NC after 3 years of follow-up. In MCI at the first 18F-FDG-PET scan, medial temporal lobe (94.7% sensitivity, 80.5% specificity) and posterior cingulate cortex (89.5% sensitivity, 73.1% specificity) hypometabolism predicted conversion with high accuracy. 18F-FDG-PET hypometabolism preceded dementia conversion at an interval of 3.70 ± 1.68 years and was earlier than volumetric changes.
Conclusions
Our analysis support the use of individual-based 18F-FDG-PET analysis to predict MCI conversion. Changes in 18F-FDG-PET occurred 1 to 8 years prior to dementia conversion, and hypometabolism occurred 2 years before MRI findings.
Investigation of AIDS-related high-risk behaviors and self-testing services among students from traditional Chinese medicine university
Authors: Ji Jingmin , Zhang Zhiqin , Yu Kun , Ye Jianya , Xu Lijun , Kong Jing , Shi Xinli
Published: December 2022

ObjectiveTo investigate the awareness of AIDS self-testing services and associated factors, as well as the cognition of AIDS-related high-risk behaviors in students of traditional Chinese medical universities.Methods: 716 sophomores participated in a questionnaire survey concerning their understanding of AIDS-related high-risk behaviors.Results: Although there had been a decline in accuracy, university students continued to have the best knowledge of HIV blood transfer, mother-to-child transmission, and sexual transmission of AIDS. More than half of the students misbelieved that the transmission was caused by an HIV-positive person sneezing or coughing (57.54%) or by a mosquito or other blood-sucking bug bites (68.44%). Ritual kissing with HIV-infected persons (52.93%) and sharing a bathroom or swimming pool with them (53.91%) were thought to be the ways that HIV spread. Male and female students had different cognition of men who have sex with men risk factors (P < 0.05). Monthly expenses (> 2000 CNY) [AOR = 5.34 (2.92–9.76)] had a higher odds of a good level of AIDS voluntary counseling and self-testing services.Conclusion: Sophomore university students need to learn professional AIDS high-risk behavior knowledge. Targeted and timely HIV risk education should be taught in college courses, focusing on AIDS self-testing education among students.
System design of student status pre-warning management based on big data
Authors: gao qianqian , Pan Dan , Xu Yurui , Li Wenjuan , Song Guangyuan
Published: December 2022

Based on the idea of Big Data and technical framework, filtered out the specific condition data displayed in a visual way to pre-warn to specific groups of students after collating and cleaning the collected data of score of students of various majors in various colleges. This paper analyzed the status and significance of school students' performance management and school status early warning. Then, we introduced big data technology, designed Hadoop-based pre-warning system to standardize the management process of intelligent digital teaching service and pre-warning system based on a large number of students' achievements. Finally, the paper puts forward the key technology of big data and the concrete implementation path of system design.
Mapping Part Characteristics to Machining Capabilities using a Matching Algorithm.
Authors: Peck William Jordan , Finke Daniel A.
Published: December 2022

The service composition problem is a major obstacle that cloud manufacturing platforms must overcome in order to match service providers to consumers such that the requirements of the consumer are met by the service provider. Previous research focuses on supplier selection, quality of service, and semantic web service approaches as a solution to the service composition problem, but rely on subjective aggregations of high-level concepts that provide a distorted perception of the service providers capabilities. Our matching algorithm methodology uses detailed information regarding operation, part, and tooling specific data to create a granular and realistic representation of the service provider. The matching algorithm is validated using a real-world part drawn from the literature and manufacturers in central Pennsylvania. Our findings indicate that the matching algorithm scales linearly as the number of inputs for a parameter increases. The research contribution
of our matching algorithm is significant as its use of detailed machine specific information is novel. Additionally, its inclusion in a cloud manufacturing platform will decrease machine idleness, while providing the customer with an on-demand service that will aid downstream production and design.
SRSF2-P95H decreases JAK/STAT signaling in hematopoietic cells and delays myelofibrosis development in mice
Authors: Solary Eric , Willekens Christophe , Laplane Lucie , Dragher Tracy , Benlabiod Camelia , Papadopoulos Nicolas , LAcout Catherine , Rameau Philippe , Catelain Cyril , Alfaro Alexia , Edmond Valerie , Signolle Nicolas , Marchand Valentine , Droin Nathalie , Hoogenboezem Remco , Schneider Rebekka , Penson Alex , Abdel-Wahab Omar , GIRAUDIER Stéphane , Pasquier Florence , Marty Caroline , Plo Isabelle , Villeval Jean Luc , Constantinescu Stefan N. , Porteu Francoise , Vainchenker William
Published: December 2022

Heterozygous mutation targeting proline 95 in Serine/Arginine-rich Splicing Factor 2 (SRSF2), associates with V617F mutation in Janus Activated Kinase 2 (JAK2) in some myeloproliferative neoplasms (MPNs), most commonly primary myelofibrosis. To explore Srsf2P95H interaction with Jak2V617F, we generated Cre-inducible knock-in mice expressing these mutants under control of the stem cell leukemia (Scl) gene promoter. In transplantation experiments, Srsf2P95H unexpectedly delayed myelofibrosis induced by Jak2V617F and decreased TGFβ1 serum level. Srsf2P95H reduced the competitiveness of transplanted Jak2V617F hematopoietic stem cells while preventing their exhaustion. RNA sequencing of sorted megakaryocytes identified an increased number of splicing events when the two mutations were combined. Focusing on JAK/STAT pathway, Jak2 exon 14 skipping was promoted by Srsf2P95H, an event detected in patients with JAK2V617F and SRSF2P95 co-mutation. The skipping event generates a truncated inactive JAK2 protein. Accordingly, Srsf2P95H delays myelofibrosis induced by the thrombopoietin receptor agonist Romiplostim in Jak2 wildtype animals. These results unveil JAK2 exon 14 skipping promotion as a strategy to reduce JAK/STAT signaling in pathological conditions.
Swyer-James-MacLeod Syndrome in a Patient With Eisenmenger Syndrome
Authors: Gürses Dolunay , Oğuz Merve , Yüksel Doğangün , Ufuk Furkan , Yılmaz Münevver
Published: December 2022

Swyer-James-MacLeod syndrome is a condition characterized radiologically by hyperlucency in a single lung lobe associated with reduced vascularity, alveolar hyperdistention, and air trapping in the absence of bronchial airway obstruction. Ventricular septal defect is the most common congenital heart defect in childhood and causes irreversible pulmonary hypertension and Eisenmenger syndrome if not treated in a timely manner. In this case report, we present a 25-year-old patient with Swyer-James-MacLeod syndrome and Eisenmenger syndrome. Including Swyer-James-MacLeod syndrome in the differential diagnosis of patients with atypically distributed pulmonary emphysema and unilateral hyperlucency is important for early diagnosis and timely treatment.
Fuzzy Radon Hazard Index Assessment for Stochastic Environmental Health Risk Evaluation of Urban Scale Building
Authors: Sarkheil Hamid , Shirkhani Dariush , Azimi Yousef , Talebi Ahmad , Rahbari Shahrokh
Published: December 2022

Radon gas emission is an emerging phenomenon that modern lifestyles have become a potential danger to humans. So, stochastic evaluation of the amount of this hazardous gas in urban scale and residential buildings can be critical in identifying environmental health risks. A novel fuzzy Radon Hazard Index is proposed in this research to assess statistical radon environmental health risks. The output index FRHI ranges from 0 (No Hazard) to 100 (The highest degree of hazard). The approach can serve as a circumstantially integrated standard for stochastic radon risk assessment and management because innovation fuzzifies this field's most important stochastic standards. In this study, first, Radon was measured in the urban scale building, and then, Arc GIS software prepared natural Radon emission zoning maps at urban and geological scales. A residential building unit in one of the critical areas has been selected. Some corrective actions have been adopted to reduce Radon in urban building units. The FRHI Assessment for stochastic environmental health risk evaluation shows that the initial fuzzy level for the mean value of FRHI is Hazardous (for FRHI value equal to 60.1) determined by Red color. This is while the Maximum FRHI level for 48 hours after installation is Rather Hazardous (for FRHI value equal to 44.8) determined with orange color, and the maximum statistical environmental health risk after steady-state installation would fall into an improved category. So identifying critical areas can provide exceptional control at the urban scale building that reduces the risks of natural Radon.
A dynamic epibiont community associated with the bone eating worm Osedax
Authors: Goffredi Shana , Panossian Balig , Brzechffa Camille , Field Naomi , King Chad , Moggioli Giacomo , Rouse Greg W. , Martín-Durán José M. , Henry Lee
Published: December 2022

BackgroundOsedax,the deep-sea annelid found at sunken whalefalls, is known to host bacterial endosymbionts intracellularly in specialized roots, that help it feed exclusively on vertebrate bones. Past studies, however, have also made mention of external bacteria on their trunks. Here, we present an examination of the bacterial communities associated with the external surfaces of seven species ofOsedaxworms. Using molecular, metagenomic, and microscopy analyses we reveal a dynamic community of Campylobacterales epibionts associated withOsedaxthat are unique from close relatives and metabolically suited to different successional stages of whale decomposition.ResultsDuring this 14-year study, we reveal a dynamic, yet persistent, succession of Campylobacterales epibionts integrated into the epidermis ofOsedax, that change over time as the whale carcass degrades on the sea floor. The epibionts associated with seven species ofOsedax, which comprise 67% of the bacterial community on the trunk, are initially dominated by the genusArcobacter(at early time points < 24 months), theSulfurospirillumat intermediate stages (~ 50 months), and theSulfurimonasat later stages (>140 months) of whale carcass decomposition. Metagenome analysis of the epibiont metabolic capabilities suggests a transition from heterotrophy to autotrophy along the successional gradient, and differences in their capacity to metabolize oxygen, carbon, nitrogen, and sulfur. Compared to free living relatives, theOsedaxepibionts were highly enriched in transposable elements, implicating genetic exchange on the host surface, and contained numerous secretions systems with enriched effector proteins having eukaryotic-like domains.ConclusionsDiverse bacteria form non-transient associations with the external surfaces of eukaryotes and can contribute to the health and physiology of their hosts. The recurrence of three Campylobacterales associated with diverseOsedaxspecies collected from multiple deep-sea locations suggests they are specific epibionts that share a long-evolutionary history with these enigmatic, yet widely distributed deep-sea worms. All three epibionts have an affinity for organic-rich and sulfide-rich habitats, however a successional shift in their composition reveals that they are a dynamic community that changes over time. These results provide evidence of a persistent yet dynamic relationship betweenOsedaxand specific Campylobacterales epibionts that possess unique genomic features.
Post-harvest protective applications of rhamnolipid producing phyllospheric Pseudomonas sp. MP 11 isolated from Musa acuminata
Authors: George Maria Liz , Theresa Mary , Geevarghese Amala , Anilkumar Athira , K Radhakrishnan E
Published: December 2022

Microorganisms residing within the diverse parts of plants play a significant role in the plant growth and defence response. Hence, the current study emphasized the isolation and screening of plant-beneficial traits of isolates obtained from leaves of Musa acuminata cultivated in Kottayam, Kerala. Among the many isolates obtained during screening, MP 11 was found to have promising activity against the tested phytopathogens viz. Fusarium rolfsii, Pythium aphanidermatum, Phytophthora infestans, Sclerotium rolfsii. This isolate was further identified as Pseudomonas sp. by the 16S rRNA-based sequence analysis. Additionally, the isolate was analysed for various plant growth promoting traits, whereby it showed its capability to solubilise zinc and phosphate, IAA, HCN and ACC deaminase production. MP 11 also showed potential to provide post-harvest protection to the rice seeds from P. aphanidermatum and S. rolfsii. Based on all these properties, the isolated PGPB could be developed into a formulation which would induce multiple beneficial impacts in the production of rice.
Can habitat preferences of ground-dwelling insects be a good indicator for terrestrial ecosystem recovery after an oil-spill?
Authors: Segev Nitzan , Groner Elli , Bouskila Amos , Berger-Tal Oded
Published: December 2022

Although oil spills are a major source of global pollution, little is known about the effects of oil spills on animals in terrestrial ecosystems. We investigated two oil spills that occurred in 1975 and 2014 in a nature reserve within a hyper-arid desert, aiming at determining the long- and short-term effects of oil spills on the habitat selection behavior of desert insects, as a possible behavioral indicator for restoration success. We performed habitat selection experiments under lab conditions, giving ground-dwelling Mesostena angustata beetles and Myrmeleon hyalinus antlion larvae choice trials between various combinations of clean and oil-contaminated soils from the new and old oil spills sites. When given a choice between clean and 2014 contaminated soil, beetles and antlions selected the clean soil. Moreover, antlions in contaminated soil from 2014 reduced their movements and did not dig pit-traps. Surprisingly, both beetles and antlions selected the 1975 contaminated soil over other soil types, and antlions dug many pit-traps in the 1975 contaminated soil.
Implications for insect conservation: The antlions' behavior reflects the status of its population in the field and can be used as an indicator for habitat restoration. In contrast, the beetle's behavior may not be a reliable behavioral indicator. Beetles were scarce in 1975 contaminated areas in the field. The disparity between individual behavior of beetles in the lab and field population distribution raised the question: Can old oil spills become an ecological trap for these species? This remains an open question for further research.
SARS-CoV2 mutations and impact on mortality: observational study in a sub- Saharan Africa hospital
Authors: Makulo Jean-Robert , Mbala Placide , Nlandu Yannick , Kabwe Benjanmin , Wumba Roger , Aziza Adrienne Amuri , Mandina Madone Ndona , Mangala Donatien , Bepouka Ben Izizag , Odio Jerome Ossam , Longokolo Murielle , Mukenge Eric K , Kamwiziku Guyguy , Kingand Eddy Lusamaki , Bashengezi Constantin , Kabanda Gilbert , Longo-Mbenza Benjamin
Published: December 2022

Background
One year after the COVID-19 pandemic, the focus of attention has shifted to the emergence and spread of SARS-CoV-2 variants of concern (VOCs). The aim of the study was to assess the frequency of VOCs in patients followed for COVID-19 at Kinshasa university hospital (KUH) during the 3rd and 4th waves of the pandemic in Kinshasa. Hospital mortality was compared to that of the first two waves.
Method
The present study was conducted from June 2021 to January 2022 and included all patients in whom the diagnosis of SARS-CoV-2 infection was confirmed by the polymerase chain reaction (PCR). The laboratory team sequenced a subset of all SARS-CoV-2 positive samples with high viral loads define as Ct < 25 to ensure the chances to generate complete genome sequence. RNA extraction was performed using the Viral RNA Mini Kit (Qiagen). Depending on the platform, we used the iVar bioinformatics or artic environments to generate consensus genomes from the raw sequencing output in FASTQ format.
Results
During the study period, the original strain of the virus was no longer circulating. The Delta VOC was predominant from June (92%) until November 2021 (3rd wave). The Omicron VOC, which appeared in December 2021, became largely predominant one month later (96%) corresponding the 4th wave. In-hospital mortality associated with COVID-19 fell during the 2nd wave (7% vs 21% 1st wave), had risen during the 3rd (16%) wave before falling again during the 4th wave (7%) (p < 0.001).
Conclusion
The Delta (during the 3rd wave) and Omicron VOCs (during the 4th wave) were very predominant among patients followed for Covid-19 in our hospital. Contrary to data in the general population, hospital mortality associated with severe and critical forms of COVID-19 had increased during the 3rd wave of the pandemic in Kinshasa.
RRCN: A Reinforced Random Convolutional Network based Reciprocal Recommendation Approach for Online Dating
Authors: Luo Linhao , Yang Liqi , Ju Xin , Fang Yixiang , Zhang Xiaofeng , Liu Kai
Published: December 2022

Recently, reciprocal recommendation, especially for online dating applications, has attracted increasing research attention. Different from conventional recommendation problems, reciprocal recommendation aims to simultaneously best match users' mutual interests. Lots of existing methods adopt user attributes and consider the interactions between attributes to capture such interest. However, these methods failed to notice that the interactions might imply both users' preference and repulsiveness to other users, which contributes opposite to the mutual interest. Moreover, the interactions grow exponentially with user attributes, posing a great challenge for distinguishing them. To address aforementioned issues, in this paper, we propose a novel reinforced random convolutional network (RRCN) approach for reciprocal recommendation. In particular, we first separately consider the preferred and repulsive interactions, where their contributions can be modeled individually. Then, we technically propose a novel random CNN component that can randomly convolute non-adjacent features and learn representations of different interaction subsets. Furthermore, we design a reinforcement learning-based strategy to integrate with the random CNN component to select important interactions for recommendation, so that the number of attribute interactions can be reduced. We evaluate the proposed RRCN against a number of both baselines and state-of-the-art approaches on two real-world datasets, and the promising results have demonstrated the superiority of RRCN against the compared approaches in terms of a number of evaluation criteria.