bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of parturition time and climatic conditions on milk productivity, milk quality and udder morphometry in Saanen goats in a semi-intensive system
Authors: Erduran Hakan
Published: December 2022

This study is the first report to investigate the relationships between time of parturition and milk productivity and quality, as well as indices related to udder measurements and meteorological variables, in Saanen goats raised under semi-intensive conditions. Goats giving birth in the hours of darkness had higher milk production than those that gave birth in the hours of daylight, while those giving birth during the evening hours had lower somatic cell count (SCC) than those with parturition during the daylight and night hours (P < 0.05). In addition, the time of parturition was associated with rear udder depth, udder circumference, and udder volume traits (P < 0.01). Parity and time of parturition × parity interaction had significant effects on lactation milk yield and lactation length, as well as milk fat, protein, lactose, total solids content and electrical conductivity (P < 0.05 to P < 0.01). The lactation stage, daily milk yield level and parity affected milk SCC (P < 0.05). Ambient temperature and daylight length had strong effects on daily milk yield (P < 0.05). These findings have practical implications for productivity, quality and health promotion efforts aimed at increasing Saanen goat dairy productivity consistently in the face of climatic changes in a semi-intensive system.
Measuring and Improving Stakeholder Welfare Is Easier Said than Done
Authors: Gurun Umit G. , Nickerson Jordan , Solomon David H.
Published: December 2022

While corporate social responsibility by firms aims at improving welfare for different social groups, whether it achieves this is often difficult to measure. After Apr. 2018 protests, Starbucks enacted policies that anybody could sit in their stores and use the bathroom without making a purchase. Using anonymized cellphone location data, we estimate this led to a 7.0% decline in attendance relative to other nearby coffee shops. The effect is 84% larger near homeless shelters and larger for Starbucks’ wealthier customers. The average time spent per visit declined by 4.1%. Public urination citations decreased near Starbucks locations, but other minor crimes were unchanged.
A Liberalization Spillover: From Equities to Loans
Authors: Liu Xin , Wei Shang-Jin , Zhou Yifan
Published: December 2022

The opening of equity markets to foreign investment by developing countries appears to generate an enormously large positive growth effect (see Bekaert, Harvey, and Lundblad (2005), Journal of Financial Economics 77, 3–55) in spite of a relatively small role of such markets for financing investment in most economies. We propose a spillover channel from equity market opening to lower costs of bank loans, which helps to explain this puzzle. From analyzing bank loan data associated with China’s introduction of the Qualified Foreign Institutional Investors program, we find significant support for this channel. Furthermore, we show that a reduction in the risk premium in loans is an important mechanism.
Anthropogenic pressure on large carnivores and their prey in the highly threatened forests of Tanintharyi, southern Myanmar
Authors: Shwe Nay Myo , Grainger Matthew , Ngoprasert Dusit , Aung Saw Soe , Grindley Mark , Savini Tommaso
Published: December 2022

The Tanintharyi Region in southern Myanmar is rich in biodiversity yet is facing threats from varying degrees of anthropogenic pressure. In this research we examine how anthropogenic pressures are influencing large carnivores (tiger Panthera tigris, leopard Panthera pardus and dhole Cuon alpinus) and their major prey species (wild pig Sus scrofa, muntjac Muntiacus spp., sambar Rusa unicolor, gaur Bos gaurus and banteng Bos javanicus) in the Lenya Reserved Forest and adjacent areas of Sundaic forest. We used data from camera-trap surveys during May 2016–March 2018 and logistic regression to analyse the relationships between the presence of large carnivores and explanatory variables such as human disturbance, landscape variability and changes in prey distribution. Tiger presence was positively associated with the occurrence of gaur and distance to villages. The occurrence of prey did not explain the detection of leopards in the study area. We suspect this was because leopards have a broad diet, including arboreal primates, and their prey was not fully recorded in our camera-trap survey. Dholes were positively associated with wild pigs and the total number of prey but not associated with forest type and landscape variables. To restore the carnivore population and conserve the biodiversity of this area, effective protection of predators and habitat management for large ungulates are crucial.
A rare case of human taeniasis caused by Taenia saginata with species undetermined cysticercosis
Authors: Hou Jie , Chen Weilin , Chen Rong , He Chunlei , Ma Ying , Qu Junyan
Published: December 2022

Taeniasis and cysticercosis, which are caused by Taenia saginata, Taenia solium and Taenia asiatica, are zoonotic parasitic infections with a significant disease burden worldwide. There is consensus amongst experts that T. saginata is a common tapeworm that causes taeniasis in humans as opposed to cysticercosis. This case study of a middle-aged Tibetan man conducted in 2021 challenges the prevailing notion that T. saginata exclusively causes taeniasis and not cysticercosis by documenting symptoms and laboratory studies related to both taeniasis and multiple cysticercosis. The patient's medical record with the symptoms of taeniasis and cysticercosis was reviewed, and the tapeworm's proglottids and cyst were identified from the patient by morphological evaluation, DNA amplification and sequencing. The patient frequently experienced severe headaches and vomiting. Both routine blood screenings and testing for antibodies against the most common parasites were normal. After anthelmintic treatment, an adult tapeworm was found in feces, and medical imaging examinations suggested multiple focal nodules in the brain and muscles of the patient. The morphological and molecular diagnosis of the proglottids revealed the Cestoda was T. saginata. Despite the challenges presented by the cyst's morphology, the molecular analysis suggested that it was most likely T. saginata. This case study suggests that T. saginata infection in humans has the potential to cause human cysticercosis. However, such a conclusion needs to be vetted by accurate genome-wide analysis in patients with T. saginata taeniasis associated with cysts. Such studies shall provide new insights into the pathogenicity of T. saginata.
To what extent does income explain the effect of unemployment on mental health? Mediation analysis in the UK Household Longitudinal Study
Authors: Thomson Rachel M. , Kopasker Daniel , Leyland Alastair , Pearce Anna , Katikireddi S. Vittal
Published: December 2022

Background

Employment and income are important determinants of mental health (MH), but the extent that unemployment effects are mediated by reduced income is unclear. We estimated the total effect (TE) of unemployment on MH and the controlled direct effect (CDE) not acting via income.

Methods

We included adults 25–64 years from nine waves of the UK Household Longitudinal Study (n = 45 497/obs = 202 297). Unemployment was defined as not being in paid employment; common mental disorder (CMD) was defined as General Health Questionnaire-12 score ≥4. We conducted causal mediation analysis using double-robust marginal structural modelling, estimating odds ratios (OR) and absolute differences for effects of unemployment on CMD in the same year, before (TE) and after (CDE) blocking the income pathway. We calculated percentage mediation by income, with bootstrapped standard errors.

Results

The TE of unemployment on CMD risk was OR 1.66 (95% CI 1.57–1.76), with 7.09% (6.21–7.97) absolute difference in prevalence; equivalent CDEs were OR 1.55 (1.46–1.66) and 6.08% (5.13–7.03). Income mediated 14.22% (8.04–20.40) of the TE. Percentage mediation was higher for job losses [15.10% (6.81–23.39)] than gains [8.77% (0.36–17.19)]; it was lowest for those 25–40 years [7.99% (−2.57 to 18.51)] and in poverty [2.63% (−2.22 to 7.49)].

Conclusions

A high proportion of the short-term effect of unemployment on MH is not explained by income, particularly for younger people and those in poverty. Population attributable fractions suggested 16.49% of CMD burden was due to unemployment, with 13.90% directly attributable to job loss rather than resultant income changes. Similar analytical approaches could explore how this differs across contexts, by other factors, and consider longer-term effects.
ZP3 and AIPL1 participate in GVBD of mouse oocytes by affecting the nuclear membrane localization and maturation of farnesylated prelamin A
Authors: Jin Zhen , Yang Qing-Mei , Xiao Chen-Yu , Gao Lei-Lei
Published: December 2022

The low maturation rate of oocytes is an important reason for female infertility and failure of assisted pregnancy. The germinal vesicle breakdown (GVBD) is a landmark event of oocyte maturation. In our previous studies, we found that zona pellucida 3 (ZP3) was strongly concentrated in the nuclear region of germinal vesicle (GV) oocytes and interacted with aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) and lamin A to promote GVBD. In the current study, we found that lamin A is mainly concentrated in the nuclear membrane. When ZP3 is knocked down, lamin A will be partially transferred to the nucleus of oocytes. The prelamin A is increased in both the nuclear membrane and nucleus, while phosphorylated lamin A (p-lamin A) is significantly reduced. AIPL1 was also proved to accumulate in the GV region of oocytes, and ZP3 deletion can significantly inhibit the aggregation of AIPL1 in the nuclear region. Similar to ZP3 knockdown, the absence of AIPL1 resulted in a decrease in the occurrence of GVBD, an increase in the amount of prelamin A, and a significant decrease in p-lamin A in oocytes developed in vitro. Finally, we propose the hypothesis that ZP3 can stabilize farnesylated prelamin A on the nuclear membrane of AIPL1, and promote its further processing into mature lamin A, therefore promoting the occurrence of GVBD. This study may be an important supplement for the mechanism of oocyte meiotic resumption and provide new diagnostic targets and treatment clues for infertility patients with oocyte maturation disorder.
Impact of the number of retrieved oocytes on IVF outcomes: oocyte maturation, fertilization, embryo quality and implantation rate
Authors: Jamil M. , Debbarh H. , Kabit A. , Ennaji M. , Zarqaoui M. , Senhaji W. R. , Hissane M. , Saadani B. , Louanjli N. , Cadi R.
Published: December 2022

The process of oocyte retrieval represents a key phase during the cycles of in vitro fertilization (IVF). It involves controlled ovarian stimulation to retrieve the highest number of oocytes possible. According to many previous studies, the higher the number of oocytes the higher the chances of obtaining embryos for multiple transfers. In this study, in total, 1987 patients were retrospectively reviewed to investigate the correlations between the number of retrieved oocytes and the subsequent IVF outcomes. Patients were divided into three groups according to the number of retrieved oocytes (Group 1: ≤5 oocytes; Group 2: 6–15 oocytes; Group 3: ≥15 oocytes). The results showed a significant negative correlation between oocyte number and maturation rate as well as fertilization rate. However, a significant positive correlation was found between oocyte number and the blastulation rate. The implantation rate after fresh embryo transfers was higher in group 2 (6–15 oocytes) compared with group 1 (≤5 oocytes). According to our findings, we conclude that oocyte numbers between 6 and 15 oocytes can result in the highest chances of positive IVF outcomes in terms of embryo quality and fresh embryo transfers with lower risks of ovarian hyperstimulation.
From free-for-all to self-governing monopoly: market organization and price information at the Amsterdam Stock Exchange, 1796–1940
Authors: de Jong Abe , Jonker Joost , Poukens Johan
Published: December 2022

For most of its history Amsterdam securities trading was entirely unregulated and spread over various venues frequented by different social groups, handicapping price transparency. A public price current emerged only in 1796 and then with wide bid-ask spreads to protect margins. To combat the confusion a curious pricing method, the mid-price system, emerged during the nineteenth century. Tied to a market microstructure centring onhoekmannen(market makers), this system transited effortlessly from a public market into a monopoly by 1913, self-governing, still without any government regulation, and offering wide rent-seeking opportunities.
Amateur Lunatics: Investigative Journalism, Asylum Reform, and the Undercover Authorship of Lewis Wingfield
Authors: Donovan Stephen , Rubery Matthew
Published: December 2022

This essay examines the literary career of Anglo-Irish peer Lewis Strange Wingfield (1842-1891) in relation to the rise of investigative journalism and Victorian debates over the treatment of mental illness. Situating his work in the context of the first covert investigations into asylums in Britain and the United States, it focuses on Wingfield's use of disguise to infiltrate a private London asylum for the purpose of researching his novel Gehenna; or, Havens of Unrest (1882). Wingfield's pioneering experiment in undercover authorship, we argue, sheds new light on investigative journalism's impact on both the form and thematics of the nineteenth-century realist novel.
Commercial urban agriculture in Florida: a qualitative needs assessment
Authors: Campbell Catherine G. , DeLong Alia N. , Diaz John M.
Published: December 2022

The global trend of urbanization coupled with an increasing awareness of the importance of food systems resilience, has led to an increasing interest in urban agriculture to sustainably feed the rapidly growing urban population and mitigate against food supply chain disruptions. While home and community gardens have been long studied, there has been relatively little empirical research focused specifically on commercial urban agriculture (CUA) operations. The purpose of this study was to characterize commercial urban farms, and to identify their primary barriers to business development and expansion, their perceptions of future opportunities, and their specific informational needs. Because CUA has received relatively less attention in previous empirical research, a qualitative approach was used for this needs assessment to collect rich, contextualized information to help differentiate the specific barriers, opportunities and needs of CUA operations as opposed to their rural counterparts. We conducted semi-structured interviews (n= 29) of CUA producers in Florida. These interviews revealed that CUA operations face many of the same barriers that are common to establishing and growing small farms, with additional barriers due to local government regulations and tensions associated with farming on land that is not traditionally used for agriculture. Despite these difficulties, CUA operators believe their urban location is a key benefit to their operation and they see a variety of opportunities for future business and market expansion.
Potential spring canola yield losses due to weeds in Canada and the United States
Authors: Geddes Charles M. , Tidemann Breanne D. , Ikley Joseph T. , Dille J. Anita , Soltani Nader , Sikkema Peter H.
Published: December 2022

Weeds represent one of the most important biotic threats to agricultural plant health, and the potential global impact of weeds on crop yields is similar to that of all other pests (animal pests and pathogens) combined. Canola is the most-grown crop in Canada based on seeded area and generates on average Can$29.9 billion in economic activity each year. The objective of this report, sponsored by the Weed Science Society of America Weed Loss Committee, was to provide an updated estimate of potential yield and monetary losses due to weed interference in spring canola grown in Canada and the United States. Quantitative yield data from field experiments were provided by researchers and weed science professionals in the northern Great Plains region; the major canola-producing area of North America. Overall, 89 yield loss estimates were compiled, covering the 18-yr period from 2003 to 2020. Average canola yield losses due to weed interference in Alberta, Saskatchewan, Manitoba, and North Dakota were 35%, 30%, 18%, and 28%, respectively. Potential yield losses weighted by canola harvested area averaged 30%, 28%, and 30% for Canada, the United States, and both countries combined, respectively. Therefore, unfettered weed interference in spring canola represents a potential monetary loss of Can$2.21 billion, $0.16 billion, and $2.37 billion for farmers in Canada, the United States, and both countries combined. The realization of such losses could manifest through continued selection for herbicide-resistant weeds, indicating the critical need for canola farmers to diversify resistance selection pressures by implementing proactive integrated weed management programs.
Simulated Impact of Ocean Alkalinity Enhancement on Atmospheric CO2 Removal in the Bering Sea
Authors: Wang Hongjie , Pilcher Darren J. , Kearney Kelly A. , Cross Jessica N. , Shugart O. Melissa , Eisaman Matthew D. , Carter Brendan R.
Published: December 2022

Ocean alkalinity enhancement (OAE) has the potential to mitigate ocean acidification (OA) and induce atmospheric carbon dioxide (CO2) removal (CDR). We evaluate the CDR and OA mitigation impacts of a sustained point‐source OAE of 1.67 × 1010 mol total alkalinity (TA) yr−1 (equivalent to 667,950 metric tons NaOH yr−1) in Unimak Pass, Alaska. We find the alkalinity elevation initially mitigates OA by decreasing pCO2 and increasing aragonite saturation state and pH. Then, enhanced air‐to‐sea CO2 exchange follows with an approximate e‐folding time scale of 5 weeks. Meaningful modeled OA mitigation with reductions of >10 μatm pCO2 (or just under 0.02 pH units) extends 100–100,000 km2 around the TA addition site. The CDR efficiency (i.e., the experimental seawater dissolved inorganic carbon (DIC) increase divided by the maximum DIC increase expected from the added TA) after the first 3 years is 0.96 ± 0.01, reflecting essentially complete air‐sea CO2 adjustment to the additional TA. This high efficiency is potentially a unique feature of the Bering Sea related to the shallow depths and mixed layer depths. The ratio of DIC increase to the TA added is also high (≥0.85) due to the high dissolved carbon content of seawater in the Bering Sea. The air‐sea gas exchange adjustment requires 3.6 months to become (>95%) complete, so the signal in dissolved carbon concentrations will likely be undetectable amid natural variability after dilution by ocean mixing. We therefore argue that modeling, on a range of scales, will need to play a major role in assessing the impacts of OAE interventions.
Seasonal Modulation of the Eddy Kinetic Energy and Subtropical Countercurrent Near the Western North Pacific Boundary
Authors: Wu Baolan , Gan Jianping
Published: December 2022

Seasonality of the subtropical countercurrent (STCC) and associated surface eddy kinetic energy (EKE) near the western North Pacific boundary is investigated. Unlike the interior STCC region where the EKE peaks in late spring (May) interpreted by the vertical velocity shear between the STCC and underlying North Equatorial Current in early spring (March), the high EKE near the western boundary lasts from May to August with unclear mechanism. Other studies suggest that the wind, heat flux and mode water could contribute to the EKE seasonality along the STCC band. But all these mechanisms can barely explain the observed EKE seasonality near the western boundary comparing to the interior STCC region, since there are no seasonal phase changes in the above forcings. We found that the eddies provide significant contribution to the EKE and STCC seasonal variation near the western boundary. The westward propagating eddies are intensified by the background shear instability near the western boundary and then further induce the high EKE in summer through the EKE convergence. At the same time, the strong surface current by the enhanced eddy activity changes the seasonality of the STCC. The dynamics is different near the western boundary from that in the interior ocean, with eddies playing more important role in modulating the EKE and STCC seasonal variation. The finding of this study will contribute to a better understanding of the dynamics of eddy‐current interaction and ocean variability near the western boundary.
A New Sea Surface Roughness Parameterization and Its Application in Tropical Cyclone Modeling
Authors: Lan Y. , Sun D. , Leng H. , Song J. , Cao X. , Dong R.
Published: December 2022

The intensity simulation of tropical cyclones (TCs) has been a long‐standing challenge for numerical models, and an accurate sea surface roughness () parameterization scheme is the key to enhance the intensity prediction. In our study, a new parameterization scheme (SD21) is proposed and applied in the Coupled Ocean‐Atmosphere‐Wave‐Sediment Transport model to simulate two super typhoons. The SD21 takes into account both the wave state and sea foam, and it is suitable for low to extreme wind conditions. The results of the eight numerical experiments show that the TC intensity and structure are sensitive to the choice of parameterization schemes. Compared with the widely used parameterization schemes, the SD21 scheme presents much better results in the simulation of the intensity and intensification speed of strong TCs. Notably, the simulation of the wind speeds generated by the SD21 is more compatible with the best track data and significantly better than that of the other schemes. Furthermore, we find that the wave state and sea foam remarkably affect the magnitude and spatial distribution of , the following two conclusions are obtained: (a) The parameterization that takes into account the wave state can reduce the excessive roughness at the TC periphery and restrict the high‐value area of the roughness to the TC‐core region. (b) The sea foam significantly decreases the roughness value in areas with 10 m wind speeds above 40 m/s.
Long‐Term Characteristics of the Meteor Radar Winds Observed at King Sejong Station, Antarctica
Authors: Song B.‐G. , Chun H.‐Y. , Song I.‐S. , Lee C. , Kim J.‐H. , Jee G.
Published: December 2022

The characteristics of the horizontal wind in the mesosphere and lower thermosphere (MLT) at King Sejong Station (KSS) in the Antarctic Peninsula are investigated using approximately 15‐year (March 2007–November 2021) meteor radar (MR) observations. The 15‐year climatology and variability of the MLT winds at KSS are analyzed for the first time, and comparisons with the observations at other Antarctic stations are performed. The short‐period oscillations (periods between 2 hr and 10 days) of the winds, including tides and several planetary wave components, are examined for each month. Temporal and altitudinal variations in the amplitudes of the semidiurnal and diurnal tides, the two largest short‐period components, are analyzed. Annual and semiannual oscillations of the wind are distinct below and above z = 90 km, respectively. The long‐term trend of the zonal wind is estimated through a multiple linear regression analysis. Statistically significant trends of the zonal wind of ∼0.5–0.8 m s−1 yr−1 appear above z = 90 km in July. To understand observed MLT wind trends, the gravity‐wave momentum flux and gravity‐wave drag (GWD) in the MLT region are estimated from the MR observations at KSS for the 15 years. Similar seasonal variations are found between the trends of the zonal winds and zonal GWDs, especially above z = 94 km. In July where a strong positive zonal wind trend appears, positive correlation between the zonal wind and zonal GWDs is found to be statistically significant above z = 94 km.
Atypically Intense and Delayed Response of the Martian Ionosphere to the Regional Dust Storm of 2016: A Study Using MAVEN Observations and Models
Authors: Mukundan Vrinda , Withers Paul , González‐Galindo Francisco , Thampi Smitha V. , Bhardwaj Anil , Felici Marianna
Published: December 2022

During Mars dust storms, atmospheric heating and expansion moves the ionospheric peak upward. Typically, peak altitude increases by no more than 10 km, and this increase occurs simultaneously with the expansion of the dust storm. However, Felici et al. (2020), https://doi.org/10.1029/2019JA027083, using the Mars Atmosphere Volatile EvolutioN (MAVEN) Radio Occultation Science Experiment (ROSE), reported an unusually large increase of ∼20 km at southern latitudes in early October 2016 during a modest dust storm. Here, we investigate why the ionospheric peak altitude increased so much in these observations. We extend the time series of ionospheric peak altitude values beyond the limited extent of the ROSE observations by applying a one‐dimensional photochemical model, in which neutral atmospheric conditions are based on in situ MAVEN Neutral Gas Ion Mass Spectrometer observations at similar latitudes and solar zenith angles to those observed by ROSE. We find that the ionospheric peak altitude was highest throughout October 2016 yet both the local and global atmospheric dust loading were greatest 1 month earlier. We hypothesize that (a) a portion of the unusually large 20 km enhancement in peak altitude and (b) the unusual delay between the greatest dust loading and the highest peak altitude were both associated with the occurrence of perihelion, which maximizes solar heating of the atmosphere, in late October 2016.
An Energetic Diagnostic of Tropical Cyclone Size in f‐Plane Simulations
Authors: Zhang Jishi , Lin Yanluan
Published: December 2022

As a major feature of tropical cyclones (TCs), controlling factors of TC outer size or size scaling remains a fundamental scientific question. The Rossby deformation radius and a natural extent associated with potential intensity have been proposed as two scalings of TC size. But neither of them satisfactorily captures the sensitivity of TC size to sea surface temperature (SST) in idealized f‐plane simulations. Inspired by the studies of the Hadley circulation, here we proposed a new TC scaling based on an energetic diagnostic scaling. TC size is primarily a ratio of the secondary circulation strength to subsidence velocity, further determined by the total atmospheric heating in the ascending area, the gross moist stability, the diabatic cooling, and the dry static stability. The former two is based on the moist energetic budget applied to the whole storm structure, while the latter two is based on the dry thermodynamic budget applied to the subsidence areas. The new scaling well captured the sensitivity of TC size to SST in idealized f‐plane simulations, partly resulted from expanded ascending area, increased surface moisture deficit, and weakened subsidence with increased SST.
Deep Learning‐Based Rapid Flood Inundation Modeling for Flat Floodplains With Complex Flow Paths
Authors: Zhou Yuerong , Wu Wenyan , Nathan Rory , Wang Q. J.
Published: December 2022

Flood inundation emulation models based on deep neural networks have been developed to overcome the computational burden of two‐dimensional (2D) hydrodynamic models. Challenges remain for flat and complex floodplains where many anabranches form during flood events. In this study, we propose a new approach to simulate the temporal and spatial variation of flood inundation for a floodplain with complex flow paths. A U‐Net‐based spatial reduction and reconstruction method (USRR) is used to find representative locations on the floodplain with complex flow paths. The water depths at these locations are simulated using one‐dimensional convolutional neural network (1D‐CNN) models, which are well‐suited to handling multivariate timeseries inputs. The flood surface is then reconstructed using the USRR method and the simulated flood depths at the representative locations. The combined 1D‐CNN and USRR method is compared with a previously developed approach based on the long short‐term memory recurrent neural network (LSTM) models and a 2D linear interpolation‐based SRR method. Compared to the LSTM model, the 1D‐CNN model is not only more accurate, but also takes less time to develop. Although both surface reconstruction methods take <1 s to produce an inundation map for a specific point in time, the USRR method is more accurate than the SRR method, leading to an increase of 5.6% in the proportion of correctly detected inundation area. The combination of 1D‐CNN and USRR can detect over 95% of the inundated area simulated using a 2D hydrodynamic model but is 98 times faster.
Analogous comparison unravels heightened antiviral defense and boosted viral infection upon immunosuppression in bat organoids
Authors: Liu Xiaojuan , Li Cun , Wan Zhixin , Chiu Man Chun , Huang Jingjing , Yu Yifei , Zhu Lin , Cai Jian-Piao , Rong Lei , Song You-qiang , Chu Hin , Cai Zongwei , Jiang Shibo , Yuen Kwok-yung , Zhou Jie
Published: December 2022

Horseshoe bats host numerous SARS-related coronaviruses without overt disease signs. Bat intestinal organoids, a unique model of bat intestinal epithelium, allow direct comparison with human intestinal organoids. We sought to unravel the cellular mechanism(s) underlying bat tolerance of coronaviruses by comparing the innate immunity in bat and human organoids. We optimized the culture medium, which enabled a consecutive passage of bat intestinal organoids for over one year. Basal expression levels of IFNs and IFN-stimulated genes were higher in bat organoids than in their human counterparts. Notably, bat organoids mounted a more rapid, robust and prolonged antiviral defense than human organoids upon Poly(I:C) stimulation. TLR3 and RLR might be the conserved pathways mediating antiviral response in bat and human intestinal organoids. The susceptibility of bat organoids to a bat coronavirus CoV-HKU4, but resistance to EV-71, an enterovirus of exclusive human origin, indicated that bat organoids adequately recapitulated the authentic susceptibility of bats to certain viruses. Importantly, TLR3/RLR inhibition in bat organoids significantly boosted viral growth in the early phase after SARS-CoV-2 or CoV-HKU4 infection. Collectively, the higher basal expression of antiviral genes, especially more rapid and robust induction of innate immune response, empowered bat cells to curtail virus propagation in the early phase of infection.
Prognostic impact of the AML ELN2022 risk classification in patients undergoing allogeneic stem cell transplantation
Authors: Jentzsch Madlen , Bischof Lara , Ussmann Jule , Backhaus Donata , Brauer Dominic , Metzeler Klaus H. , Merz Maximilian , Vucinic Vladan , Franke Georg-Nikolaus , Herling Marco , Platzbecker Uwe , Schwind Sebastian
Published: December 2022

For most patients with acute myeloid leukemia (AML), an allogeneic hematopoietic stem cell transplantation (HSCT) offers the highest chance of cure. Recently, the European LeukemiaNet (ELN) published updated recommendations on the diagnosis and risk classification in AML based on genetic factors at diagnosis as well as a dynamic adjustment (reclassification) according to the measurable residual disease (MRD) status for the favorable and intermediate risk groups. Validation of the ELN2022 risk classification has not been reported. We retrospectively analyzed 522 AML patients who received an HSCT at a median age of 59 (range 16–76) years. For patients with adequate material available and in remission prior to HSCT (n = 229), the MRD status was evaluated. Median follow-up after HSCT was 3.0 years. ELN2022 risk at diagnosis was in 22% favorable, in 26% intermediate, and in 52% adverse. ELN2022 risk at diagnosis is associated with the cumulative incidence of relapse/progression (CIR), event-free survival (EFS), and overall survival (OS) in the whole patient cohort, as well as the subgroup of patients transplanted in first remission. However, the risk stratification based on the ELN2022 classification did not significantly improve outcome prognostication in comparison to the ELN2017 classification. In our study, the newly added group of patients with myelodysplasia-related gene mutations did not have adverse outcomes. Re-classifying these patients into the intermediate risk group and adjusting the grouping for all AML patients by MRD at HSCT, led to a refined and improved risk stratification, which should be validated in independent studies.
Glucocorticoid-induced microRNA-378 signaling mediates the progression of pancreatic cancer by enhancing autophagy
Authors: Liu Li , Han Shanshan , Xiao Xi , An Xuefeng , Gladkich Jury , Hinz Ulf , Hillmer Stefan , Hoppe-Tichy Torsten , Xu Yi , Schaefer Michael , Strobel Oliver , Herr Ingrid
Published: December 2022

Glucocorticoids (GCs) are widely used in tumor therapy to reduce tumor growth, inflammation, edema, and other side effects. Controversially, GCs may also cause the progression of highly aggressive pancreatic ductal adenocarcinoma (PDAC). Because microRNA (miR) and autophagy signaling support the invasive growth of PDAC, we asked whether these mechanisms may be targeted by GCs. Six established human PDAC cell lines, tissue from patients who received GC medication (n = 35) prior to surgery, or not (n = 35), and tumor xenografts were examined by RT‒qPCR, transmission electron microscopy (TEM), monodansylcadaverine (MDC) staining, immunohistochemistry, in situ hybridization, gene array and Kaplan‒Meier analysis with bioinformatics, and MTT, western blot, colony, spheroid, migration, and invasion assays. We found that various GCs, including dexamethasone (DEX), induced typical features of macroautophagy with the appearance of autolysosomes, enhanced LC3-II, decreased SQSTM1/p62 expression and induced epithelial-mesenchymal transition (EMT) and gemcitabine resistance. The GC receptor (GR) antagonist mifepristone (RU486) counteracted DEX-induced autophagy features, suggesting that the GC-GR complex is involved in the induction of autophagy. The autophagy-related miR-378i and miR-378a-3p were selected as the top upregulated candidates, and their high expression in PDAC patient tissue correlated with low survival. siRNA-mediated downregulation of miR-378 inhibited DEX-induced autophagy, and tumor progression. Bioinformatics confirmed the contribution of miR-378 to the regulation of signaling networks involved in GC-induced autophagy and tumor progression. The construction of a molecular docking model revealed stable binding of miR-378 to the DEX-GR complex, suggesting direct regulation. These substantial, novel, in-depth data reveal that GCs favor autophagy-mediated cancer progression by inducing miR-378 and GR binding and implicate GR and miR-378 as new therapeutic targets.
Ultrasensitive rapid cytokine sensors based on asymmetric geometry two-dimensional MoS2 diodes
Authors: De Silva Thushani , Fawzy Mirette , Hasani Amirhossein , Ghanbari Hamidreza , Abnavi Amin , Askar Abdelrahman , Ling Yue , Mohammadzadeh Mohammad Reza , Kabir Fahmid , Ahmadi Ribwar , Rosin Miriam , Kavanagh Karen L. , Adachi Michael M.
Published: December 2022

The elevation of cytokine levels in body fluids has been associated with numerous health conditions. The detection of these cytokine biomarkers at low concentrations may help clinicians diagnose diseases at an early stage. Here, we report an asymmetric geometry MoS2 diode-based biosensor for rapid, label-free, highly sensitive, and specific detection of tumor necrosis factor-α (TNF-α), a proinflammatory cytokine. This sensor is functionalized with TNF-α binding aptamers to detect TNF-α at concentrations as low as 10 fM, well below the typical concentrations found in healthy blood. Interactions between aptamers and TNF-α at the sensor surface induce a change in surface energy that alters the current-voltage rectification behavior of the MoS2 diode, which can be read out using a two-electrode configuration. The key advantages of this diode sensor are the simple fabrication process and electrical readout, and therefore, the potential to be applied in a rapid and easy-to-use, point-of-care, diagnostic tool.
Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles
Authors: Vettkötter Dennis , Schneider Martin , Goulden Brady D. , Dill Holger , Liewald Jana , Zeiler Sandra , Guldan Julia , Ateş Yilmaz Arda , Watanabe Shigeki , Gottschalk Alexander
Published: December 2022

Acutely silencing specific neurons informs about their functional roles in circuits and behavior. Existing optogenetic silencers include ion pumps, channels, metabotropic receptors, and tools that damage the neurotransmitter release machinery. While the former hyperpolarize the cell, alter ionic gradients or cellular biochemistry, the latter allow only slow recovery, requiring de novo synthesis. Thus, tools combining fast activation and reversibility are needed. Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs). We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons. optoSynC clusters SVs, observable by electron microscopy. Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off. optoSynC can inhibit exocytosis for several hours, at very low light intensities, does not affect ion currents, biochemistry or synaptic proteins, and may further allow manipulating different SV pools and the transfer of SVs between them.
Rational enzyme design for enabling biocatalytic Baldwin cyclization and asymmetric synthesis of chiral heterocycles
Authors: Li Jun-Kuan , Qu Ge , Li Xu , Tian Yuchen , Cui Chengsen , Zhang Fa-Guang , Zhang Wuyuan , Ma Jun-An , Reetz Manfred T. , Sun Zhoutong
Published: December 2022

Chiral heterocyclic compounds are needed for important medicinal applications. We report an in silico strategy for the biocatalytic synthesis of chiral N- and O-heterocycles via Baldwin cyclization modes of hydroxy- and amino-substituted epoxides and oxetanes using the limonene epoxide hydrolase from Rhodococcus erythropolis. This enzyme normally catalyzes hydrolysis with formation of vicinal diols. Firstly, the required shutdown of the undesired natural water-mediated ring-opening is achieved by rational mutagenesis of the active site. In silico enzyme design is then continued with generation of the improved mutants. These variants prove to be versatile catalysts for preparing chiral N- and O-heterocycles with up to 99% conversion, and enantiomeric ratios up to 99:1. Crystal structural data and computational modeling reveal that Baldwin-type cyclizations, catalyzed by the reprogrammed enzyme, are enabled by reshaping the active-site environment that directs the distal RHN and HO-substituents to be intramolecular nucleophiles.
Number of simultaneously acting global change factors affects composition, diversity and productivity of grassland plant communities
Authors: Speißer Benedikt , Wilschut Rutger A. , van Kleunen Mark
Published: December 2022

Plant communities experience impacts of increasing numbers of global change factors (e.g., warming, eutrophication, pollution). Consequently, unpredictable global change effects could arise. However, information about multi-factor effects on plant communities is scarce. To test plant-community responses to multiple global change factors (GCFs), we subjected sown and transplanted-seedling communities to increasing numbers (0, 1, 2, 4, 6) of co-acting GCFs, and assessed effects of individual factors and increasing numbers of GCFs on community composition and productivity. GCF number reduced species diversity and evenness of both community types, whereas none of the individual factors alone affected these measures. In contrast, GCF number positively affected the productivity of the transplanted-seedling community. Our findings show that simultaneously acting GCFs can affect plant communities in ways differing from those expected from single factor effects, which may be due to biological effects, sampling effects, or both. Consequently, exploring the multifactorial nature of global change is crucial to better understand ecological impacts of global change.
Bioenergetics of pollen tube growth in Arabidopsis thaliana revealed by ratiometric genetically encoded biosensors
Authors: Liu Jinhong , Lim Shey-Li , Zhong Jia Yi , Lim Boon Leong
Published: December 2022

Pollen tube is the fastest-growing plant cell. Its polarized growth process consumes a tremendous amount of energy, which involves coordinated energy fluxes between plastids, the cytosol, and mitochondria. However, how the pollen tube obtains energy and what the biological roles of pollen plastids are in this process remain obscure. To investigate this energy-demanding process, we developed second-generation ratiometric biosensors for pyridine nucleotides which are pH insensitive between pH 7.0 to pH 8.5. By monitoring dynamic changes in ATP and NADPH concentrations and the NADH/NAD+ ratio at the subcellular level in Arabidopsis (Arabidopsis thaliana) pollen tubes, we delineate the energy metabolism that underpins pollen tube growth and illustrate how pollen plastids obtain ATP, NADPH, NADH, and acetyl-CoA for fatty acid biosynthesis. We also show that fermentation and pyruvate dehydrogenase bypass are not essential for pollen tube growth in Arabidopsis, in contrast to other plant species like tobacco and lily.
Self-rolling of vanadium dioxide nanomembranes for enhanced multi-level solar modulation
Authors: Li Xing , Cao Cuicui , Liu Chang , He Wenhao , Wu Kaibo , Wang Yang , Xu Borui , Tian Ziao , Song Enming , Cui Jizhai , Huang Gaoshan , Zheng Changlin , Di Zengfeng , Cao Xun , Mei Yongfeng
Published: December 2022

Thermochromic window develops as a competitive solution for carbon emissions due to comprehensive advantages of its passivity and effective utilization of energy. How to further enhance the solar modulation ($${\triangle T}_{{{{{{{\rm{sol}}}}}}}}$$

△
T

sol

) of thermochromic windows while ensuring high luminous transmittance ($${T}_{{{{{{{\rm{lum}}}}}}}}$$

T

lum

) becomes the latest challenge to touch the limit of energy efficiency. Here, we show a smart window combining mechanochromism with thermochromism by self-rolling of vanadium dioxide (VO2) nanomembranes to enhance multi-level solar modulation. The mechanochromism is introduced by the temperature-controlled regulation of curvature of rolled-up smart window, which benefits from effective strain adjustment in VO2 nanomembranes upon the phase transition. Under geometry design and optimization, the rolled-up smart window with high $${\triangle T}_{{{{\rm{sol}}}}}$$

△
T

sol

and $${T}_{{{{{{\rm{lum}}}}}}}$$

T

lum

is achieved for the modulation of indoor temperature self-adapted to seasons and climate. Furthermore, such rolled-up smart window enables high infrared reflectance after triggered phase transition and acts as a smart lens protective cover for strong radiation. This work supports the feasibility of self-rolling technology in smart windows and lens protection, which promises broad interest and practical applications of self-adapting devices and systems for smart building, intelligent sensors and actuators with the perspective of energy efficiency.
Hedgehog-mediated gut-taste neuron axis controls sweet perception in Drosophila
Authors: Zhao Yunpo , Khallaf Mohammed A. , Johansson Emilia , Dzaki Najat , Bhat Shreelatha , Alfredsson Johannes , Duan Jianli , Hansson Bill S. , Knaden Markus , Alenius Mattias
Published: December 2022

Dietary composition affects food preference in animals. High sugar intake suppresses sweet sensation from insects to humans, but the molecular basis of this suppression is largely unknown. Here, we reveal that sugar intake in Drosophila induces the gut to express and secrete Hedgehog (Hh) into the circulation. We show that the midgut secreted Hh localize to taste sensilla and suppresses sweet sensation, perception, and preference. We further find that the midgut Hh inhibits Hh signalling in the sweet taste neurons. Our electrophysiology studies demonstrate that the midgut Hh signal also suppresses bitter taste and some odour responses, affecting overall food perception and preference. We further show that the level of sugar intake during a critical window early in life, sets the adult gut Hh expression and sugar perception. Our results together reveal a bottom-up feedback mechanism involving a “gut-taste neuron axis” that regulates food sensation and preference.
A robust pipeline for ranking carrier frequencies of autosomal recessive and X-linked Mendelian disorders
Authors: Zhu Wenjuan , Wang Chen , Mullapudi Nandita , Cao Yanan , Li Lin , Lo Ivan Fai Man , Tsui Stephen Kwok-Wing , Chen Xiao , Lei Yong , Gu Shen
Published: December 2022

Single gene disorders are individually rare but collectively common leading causes of neonatal and pediatric morbidity and mortality. Both parents or the mothers of affected individuals with autosomal recessive or X-linked recessive diseases, respectively, are carrier(s). Carrier frequencies of recessive diseases can vary drastically among different ethnicities. This study established a robust pipeline for estimating and ranking carrier frequencies of all known 2699 recessive genes based on genome-wide sequencing data in healthy individuals. The discovery gnomAD cohort contained sequencing data on 76,156 genomes and 125,748 exomes from individuals with seven ethnicity backgrounds. The three validation cohorts composed of the SG10K Project with 4810 genomes on East Asian and South Asian, the ChinaMAP project with 10,588 Chinese genomes, and the WBBC pilot project with 4480 Chinese genomes. Within each cohort, comprehensive selection criteria for various kinds of deleterious variants were instituted, including known pathogenic variants (Type 1), presumably loss-of-function changes (Type 2), predicted deleterious missense variants (Type 3), and potentially harmful in-frame INDELs (Type 4). Subsequently, carrier frequencies of the 2699 genes were calculated and ranked based on ethnicity-specific carrier rates of Type 1 to Type 4 variants. Comparison of results from different cohorts with similar ethnicity background exhibited high degree of correlation, particularly between the ChinaMAP and the WBBC cohorts (Pearson correlation coefficient R = 0.92), confirming the validity of our variant selection criteria and the overall analysis pipeline.
Measuring magic on a quantum processor
Authors: Oliviero Salvatore F. E. , Leone Lorenzo , Hamma Alioscia , Lloyd Seth
Published: December 2022

Magic states are the resource that allows quantum computers to attain an advantage over classical computers. This resource consists in the deviation from a property called stabilizerness which in turn implies that stabilizer circuits can be efficiently simulated on a classical computer. Without magic, no quantum computer can do anything that a classical computer cannot do. Given the importance of magic for quantum computation, it would be useful to have a method for measuring the amount of magic in a quantum state. In this work, we propose and experimentally demonstrate a protocol for measuring magic based on randomized measurements. Our experiments are carried out on two IBM Quantum Falcon processors. This protocol can provide a characterization of the effectiveness of a quantum hardware in producing states that cannot be effectively simulated on a classical computer. We show how from these measurements one can construct realistic noise models affecting the hardware.