bluuebunny/crossref_metadata_2025 huggingface dataset:
Testing output variables for sensitivity study of nonlinear vibration systems
Authors: Hajdu Flóra , Molnárka Győző
Published: August 2020

In this study the detailed One-at-a-Time sensitivity analysis of nonlinear mass spring-damper systems is carried out with numerical simulation. The degree of sensitivity was measured with a sensitivity index and based on its sensitivity Fuzzy-sets were established. The sensitivity of a parameter then can be expressed by the membership to the Fuzzy-sets. In this study the root mean square of acceleration, the maximum amplitude of acceleration and the peak frequency were chosen as output variables to measure sensitivity. With this research it was proven, that the root mean square of acceleration and the peak frequency can be used for sensitivity study of nonlinear vibration systems effectively.
Linear and nonlinear dynamical analysis of a Crane model
Authors: Maourane Hmoumen , Szabó Tamás
Published: August 2020

This paper deals with the dynamical analysis of a crane model. Truss finite elements are used to discretize the suspending chains with the so called updated Lagrangian description. This nonlinear model is regarded to be the best approximation to which linear models are compared. The inertia and independent degrees of freedom are also taken into consideration by linear models. The goal is to find a linear model, which can be used as an observer in antisway control of a crane.
Molecular characterization of submergence tolerance genes and locus in the deep-water rice cultivars
Authors: Cuu Nguyen Van , Khiem Nguyen Van , Hoi Pham Xuan
Published: August 2020

Most of the rice cultivars exhibit suspension of growth when submerged to overcome the reduced availability of oxygen. When the situation continues, majority of the cultivars unable to recover after the flood recedes. However, there are fortunately some rice genotypes that can withstand such submerged condition for up to two weeks by adapting two totally opposite mechanisms. One type of cultivars elongates enormously at a very short span of time and the leaves come above the water level. In the second type, they remain under water without any growth. Cultivars of both types tolerate the submergence but the first category easily lodges when flood water recede. In those lines, yields are reduced drastically. In this study, we focus on characterize the genetic variation at the Sub1 locus and to associate its relevance, if any, to submergence tolerance among the deep water landraces. As a first step, seeds of some rice cultivars collected from North-east Indian regions were initially selected for the characterization of genetic variation. The PCR based analysis involving several genes known to be associated with submergence tolerance did not reveal much difference. However, Southern hybridization revealed certain differences between submergence tolerant and susceptible cultivars. Although we did not notice major difference with regard to Sub1 genes when tried with EcoRI and BamHI, differences were noticed with adh1 and RAmy3C genes. Representative, Southern analysis showed the genetic variation among the deep-water cultivars as compared to Swarna and Sub1-Swarna. It is possible that deep-water rice cultivars may not differ in their genome at Sub1 locus but they respond through SNORKEL genes under submergence.
Effect of plant growth regulators on growth and lipid accumulation of microalgal Haematococcus pluvialis Flotow in two-stage culture
Authors: Dong Phuong Nguyen Tran , Ai Thuy Le Huyen , Trang Viet Bui
Published: August 2020

Haematococcus pluvialis cells were cultured in aerated liquid Bold’s Basal medium in two-stage (initial stage during in 7 weeks for increased biomass growth and second stage during in 3 weeks for increased lipid accumulation) with different volumes 250 mL, 10 L, and 1,000 L. With a volume of 250 mL, the medium was supplied with benzyl adenine (BA), indole-3-acetic acid (IAA) or gibberellic acid (GA3) at concentration from 0.1 - 0.2 mg/L in initial stage and IAA or GA3 at concentration from 0.1 - 0.2 mg/L in second stage. After 10 weeks of culture, results showed that supplement of 0.1 mg/L BA in initial stage and 0.125 mg/L IAA in second stage increased cell density, and microalgal cells had green color with a spherical shape. On the contrary, supplement of 0.15 mg/L IAA in initial stage and 0.175 mg/L GA3 in second stage increased lipid accumulation, and microalgal cells had red color with a spherical shape. With a volume of 10 L, the medium was supplied with 0.1 mg/L BA in initial stage, and treated with separation or combination from 2 - 3 of these factors (nitrogen starvation, 0.5% NaCl, 4.98 mg/L FeSO4) were applied in second stage. The result showed that the cultures was treated with nitrogen starvation increased dry biomass and biofuel, but treated with 4.98 mg/L FeSO4 only increased biofuel. With a volume of 1,000 L, microalgal cells were cultured in BB liquid medium in initial stage, and treated with 4.98 mg/L FeSO4 increased fresh 78.67 mg/mL and dry biomass 2.05 mg/L and total lipid content 28.24 %/ DW.
Characterization of O-glycan binding lectin from the red alga Hydropuntia eucheumatoides
Authors: Dinh Hung Le , Hai Yen Tran Thi , Thanh Trung Dinh
Published: August 2020

The red alga, Hydropuntia eucheumatoides is one of the algal genera from which agar is commercially extracted, and is the main source of agar in the world. The lectin HEL from the red alga H. eucheumatoides was isolated by a combination of aqueous ethanol extraction, ethanol precipitation, ion exchange and filtration chromatography. Lectin gave a single band with molecular mass of 17,000 Da in both non-reducing and reducing SDS-PAGE conditions, therefore lectin exists in monomeric form. The hemagglutination activities of HEL were stable over a wide range of pH from 3 to 10, temperature up 60 oC and not affected by either the presence of EDTA or addition of divalent cations, indicating that lectin requires no metal for biological activity. The hemagglutination activities of HEL were not inhibited by monosaccharides and glycoproteins, D-glucose, D-mannose, D-galactose, D-xylose, N-acety-D-mannosamine, transferin, fetuin and yeast mannan, but strongly inhibited by monosaccharides containing  acetamido groups at equatorial C2 position, such as N-acetyl-galactosamine, N-acetyl-glucosamine, N-acetyl-neuraminic acid and glycoprotein porcine stomach mucin bearing O-glycans. Thus, lectin is specific for O-glycans and  may recognize the sequences GalNAcαSer/Thr, GalNAc(α1-3)[Fuc(α1-2)]Gal(β1-4)GlcNAc(β1-3)GalNAc- and GluNAc(α1-4)Gal- under interacting with the acetamido groups at equatorial C2 position of the terminal sugar residues in oligosaccharide structures of O-glycans. The red alga H. eucheumatoides could promise to be a source of valuable lectins for application in biochemistry and biomedicine.
Investigating the diversity of arbuscular mycorrhizal fungi from Gymnema sylvestre and Curcuma longa in Vietnam
Authors: Kim Chi Hoang , Nhu Hang Tran Thi , Hong Ha Tran Thi , Huu Cuong Le , Ho Quang Tran , Anh Van Bui , Hoang Yen Le Thi , Mai Huong Le
Published: August 2020

Arbuscular mycorrhizal (AM) fungi are soil eukaryotes that belong to phylum Glomeromycota and have symbiosis with the vast majority of higher plants’ roots. AM fungi are believed to be coevolved with terrestrial plants, the abundance and diversity of AM fungal communities as a result are host plant dependent. A survey of AM fungi from the rhizospheres of medicinal plants in Northern Vietnam including gurma Gymnema sylvestre and turmeric Curcuma longa was carried out. From the extracted total DNAs of the medicinal plants’ rhizosphere soil samples, 35 mycorrhizal fungal species were identified by analyzing small subunit rRNA gene sequences. Result revealed that genus Glomus is the most abundant in the AM communities of G. sylvestre and C. longa, followed by Gigaspora and Acaulospora. Besides, AM species belonging to genera Scutellospora, Diversispora and Rhizophagus were observed in almost all rhizosphere soil samples. The spore counting by wet sieving and decanting method uncovered a variation in AM spore density of gurma and turmeric rhizosphere. In general, AM species were found more abundantly and more diverse in collected rhizome soil samples of C. longa (27 species belonging to 10 genera) than of G. sylvestre (17 species found belonging to 7 genera). The observed difference in AM communities of G. sylvestre and C. longa supports evidence for the dependence of AM fungal species on host plants, and indicates that AM fungi may have relation to the host plants’ secondary metabolite production.
Evaluation of genetic diversity and DNA fingerprinting of 19 standard reference rice varieties using SSR markers
Authors: Long Tran , Minh Cuc Luu , Quang Sang Nguyen , Xuan Hoi Pham
Published: August 2020

Molecular markers are advanced-tools for identifying new varieties at DNA levels. According to the International Union for the Protection of New Varieties ofPlants,  new breeded varieties need to be tested for the Distinctness, Uniformity and Stability (DUS), before being recognized as the new ones. Traditional DUS criteria based on 62 - 65 morphological and biochemical characteristics, which evaluated on comparison of new varieties with 19 standard reference varieties for traits of interest.  Study on the genotypic polymorphism of 19 standard reference rice varieties provides genotypic information of these varieties for the evaluation of new rice varieties based on genotyping analysis.  The reference marker set (30 markers) was used to evaluate the genetic diversity and DNA fingerprinting of 19 standard reference rice varieties. The results showed the similarity coefficient of 19 varieties varied from 0.04 to 0.548. At the genetic similarity coefficient of 0.1, the 19 rice varieties divided into two main groups. Group one included 3 varieties: DH1, DH5, DH13. Group 2 included the remaining 16 varieties. Inside group two, phylogenetic tree divided into two main branches at the genetic similarity coefficient of 0.3. Branch 1 includes 5 varieties including DH2, DH6, DH10, DH11 and DH7. The 11 remaining varieties were in the branch 2. The most closely varieties were DH6 and DH10 with the genetic similarity coefficient of 0.548. This study shows that, the standard reference varieties have high uniformity and high genotypic polymorphism, could used for testing new varieties based on genotyping by DNA fingerprinting combining with phenotype.
Antimicrobial activity of natural compounds from sponge – derived fungus Aspergillus flocculosus 01NT.1.1.5
Authors: Trinh Phan Thi Hoai , Thanh Van Tran Thi , Minh Ly Bui , Kyu Choi Byeoung , Jae Shin Hee , Seok Lee Jong , Seung Lee Hyi , Quyet Tien Phi
Published: August 2020

The Aspergillus fungi have been an important source of natural products that are useful for exploration in medicine, agriculture and industry. In our continuous investigation to search for new antimicrobial agents from marine-derived fungi, one new phomaligol A2 (1), together with three known compounds, wasabidienone E (2), aspertetranone D (3) and mactanamide (4), were obtained from the EtOAc extract of the culture medium of the marine-derived fungus Aspergillus flocculosus (A. flocculosus) 01NT.1.1.5 isolated from the sponge Stylissa sp. at Nhatrang Bay, Vietnam. Their chemical structures were elucidated by analysis of 1D and 2D NMR and mass spectroscopic data, as well as by comparison of the corresponding data to those previously reported in the literature. Furthermore, the aim of this study was also to evaluate the antimicrobial activity of these compounds against pathogenic microbes including Escherichia coli (E. coli) ATCC 25922, Pseudomonas aeruginosa (P. aeruginosa) ATCC 27853, Staphylococcus aureus (S. aureus) ATCC 25923, Bacillus cereus (B. cereus) ATCC 11778, Streptococcus faecalis (S. faecalis) ATCC 19433, Listeria monocytogenes (L. monocytogenes) ATCC 19111, and Candida albicans (C. albicans) ATCC 10231. Among the compounds, 1-3 were inhibitory on the growth of the yeast C. albicans with minimum inhibitory concentration (MIC) value of 16 μg/mL, which was more potent than amoxicillin and cefotaxime (MIC > 256 μg/mL), antimicrobial drugs as positive references. Moreover, compounds 1-4 were also found to be active against other pathogens including P. aeruginosa and S. faecalis with MIC values of 16 μg/mL and 32 μg/mL, respectively. Compound 4 had no inhibitory activity against L. monocytogenes, whereas compounds 1-3 had ability to against this strain with MICs of 32 to 64 μg/mL. Four of tested compounds exhibited antibacterial activity against B. cereus and E. coli with MIC values of 64-128 μg/mL. This is the first report about these compounds with antimicrobial activity obtained from marine fungus A. flocculosus isolated in Vietnam.
Current status of healthcare-associated enteroviral (non-polio) infections
Authors: Alimov A. V. , Igonina E. P. , Feldblyum I. V. , Chalapa V. I. , Zakharova Yu. A.
Published: August 2020

Here we present the data on foreign research publications describing healthcare-associated enteroviral (nonpolio) infections (HAI) sought in the Worldwide Database for Nosocomial Outbreaks (Institut für Hygiene und Umweltmedizin, Universitȁtmedizincomplex “Charite”, Germany) as well as PubMed search engine (The United States National Library), covering 1936–2017 timeframe. The publications retrieved contained the data on 28 nosocomial outbreaks caused by Enterovirus A (EV-A71), В (Echoviruses 11, 17, 18, 30, 31, 33, Coxsackie viruses А9, В2, В5) and D (EV-D68). It was discovered that the majority of the nosocomial enteroviral (non-polio) outbreaks occurred in obstetric hospitals and neonatal units so that children were mainly maternally infected. In addition, a case associated with intrauterine infection was described. It was shown that outbreaks might be started by an infected child at the incubation period. Single publications reported nosocomial outbreaks in geriatric hospitals. Generally, nosocomial enteroviral (non-polio) outbreaks were characterized by polymorphic clinical picture caused by any certain pathogen serotype and within a single site of the infection. Few lethal outcomes were recorded. Enterovirus B species dominated among identified etiological agents. Violated hospital hygiene and infection control contributing to spread of infection were among those found in neonatal units: putting used diapers out on baby bed prior disposal, sharing bathtub, toys and household objects as well as poor hand hygiene in medical workers. One of the measures recommended to improve diagnostics of enteroviral (non-polio) infections was virology screening of children with suspected sepsis in case of unidentified etiology. It was established that etiological decoding of nosocomial outbreaks was impossible without applying pathogen-specific diagnostic tools, mainly nested RT-PCR and direct sequencing of followed by subsequent phylogenetic analysis.
Balance of CD4+IFNγ+ and CD4+CD25hiT cells as early predictor of a 3-month outcome in ischemic stroke patients
Authors: Morozov S. A. , Tikhonova M. A. , Pronkina N. V. , Shtobbe A. A. , Leplina O. Yu. , Shevela E. Ya. , Ostanin A. A. , Chernykh E. R.
Published: August 2020

Early prediction for ischemic stroke (IS) outcome is a major challenge since it may help to optimize treatment program and  to make  it more  personalized. Since  T cells with  regulatory activity  are involved  in different  pathophysiological processes  in brain  stroke,  including inflammation, immune suppression, brain damage  and  repair, the  study  of T cells as potential biomarkers has essential  importance. The  present  work aimed to study the circulating T cell subsets with phenotype of type 1 T helper cells (Th1) and regulatory T cells (Treg), and their  ratio during  the acute  phase of IS, depending on stroke severity, inflammatory response  and 3-month outcome (according to modified Rankin scale, mRs).  Patients and methods. The study included 61 patients with a newly diagnosed IS (severity according to NIHSS ≥ 5), in the first 24-48 h after stroke onset, and 20 age/sex-related healthy  donors. Laboratory examination included assessment of leukocytosis, neutrophillymphocyte ratio  (NLR) and CRP  concentration. Mononuclear cells were isolated  from peripheral blood  to study T cell subsets. Th1 and Tregs were measured by FACS  analysis as CD4+IFNγ+  and CD4+CD25hiT cells, respectively. During the first 24-48 h after stroke, the patients had elevated values of leukocyte counts, NLR and CRP. Higher  levels of these parameters in severe stroke compared with mild stroke, as well as direct correlation of NIHSS with  NLR  and  CRP  evidenced that  the  stroke  severity  was associated with  more  pronounced inflammatory response. Patients were also characterized by a significant  decrease in CD4+IFNγ+Th1  cells, an  increase  in CD4+CD25hiTreg, and  a marked  decrease in Th1/Treg ratio.  Furthermore, in patients with NIHSS ≥ 8 (moderate and severe stroke), the percentage of CD4+IFNγ+T cells was in direct  correlation, and the number of CD4+CD25hiT cells was inversely related to CRP  and NLR  values. The changes of T cell subsets were more  pronounced in patients with  a favorable  3-month outcome (mRs  > 3). As a result,  the  patients with poor outcome (mRs  ≤ 3) had higher  CD4+IFNγ+T cell proportion, lower CD4+CD25hiT cell percentage and  4-fold  higher  CD4+IFNγ+/CD4+CD25hi  ratio  compared with opposing  group.  ROC  analysis  revealed  a “good” quality of prognosis  based on evaluation of the CD4+IFNγ+/CD4+CD25hi  ratio as a monopredictor of adverse outcome (AUC  = 0.75) and “very good”  quality  of prognosis  when the indicated ratio was combined with  NIHSS scale  (AUC  = 0.82).  The  data  obtained suggest  that  a decrease of Th1/Тreg ratio,  due  to  a decrease in CD4+IFNγ+  and increased CD4+CD25hiT cell counts  during the acute  phase of ischemic stroke is a compensatory reaction directed at inhibition of inflammatory response, and has a prognostic significance as early predictor of the outcome at 3 months.
Development of ELISA test for the quality control of Pseudomonas aeruginosa recombinant vaccine based on the hybrid recombinant protein
Authors: Soldatenkova A. V. , Kudryashova A. M. , Gavrilova N. F. , Yakovleva I. V. , Borisova O. V. , Sviridov V. V. , Mikhailova N. A.
Published: August 2020

A hybrid recombinant protein containing the amino  acid sequences of the three  most significant Pseudomonas aeruginosa antigens  (membrane proteins OprF, OprI  and toxoid  aTox)  was incorporated into a vaccine against Pseudomonas infection. Quality control of a hybrid recombinant protein and appropriate vaccine includes  determination of authentity and completeness of adsorption upon aluminum hydroxide adjuvant. The aim of our study was to develop  techniques of quality  control for a vaccine  based on the hybrid  OprF-aToxOprI  recombinant protein specific  to  P. aeruginosa.  Hybridomas secreting  specific  monoclonal antibodies for OprF-aTox-OprI were derived  from the fusion of myeloma cells and murine spleen  cells immunized with recombinant proteins P. aeruginosa. To  produce sufficient  quantities of antibodies, the  hybrid  cells were in vivo cultured in BALB/c mice.  Supernates and ascite liquids were chromatographically purified  with immune sorbent. Conjugation of antibodies with  horseradish peroxidase was carried  out  according to  P.K.Nakane. The  hybrid  OprF-aTox-OprI recombinant protein was detected by the  solid-phase ELISA, using a panel  of monoclonal antibodies and  conjugates of monoclonal antibodies with  horseradish peroxidase. Monoclonal antibodies were specific for different  OprF-aTox-OprI epitopes. Titration assays containing OprF-aTox-OprI protein at 78 ng/ml to 5000 ng/ml were used as quantitative standards for calibration curves.

To identify  the recombinant protein OprF-aTox-OprI, 55 variants  of of MAb pairs were tested.  Limits  of quantitative detection served  for selection of most  sensitive  and  specific  ELISA  variants.  The  quantitative detection limit was calculated for all 11 ELISA  variants.  Two ELISA  variants  with the highest  sensitivity were selected  for  quality  control of the  hybrid  recombinant protein. The  limits  of quantitative detection were, respectively, 2.9 and 13.6 ng/ml (0.0058  and 0.027% of the estimated antigen  content in the vaccine)  for the first and  second  ELISA  variants.  The  first variant  included a pair  of monoclonal antibodies specific  for the OprF  and OprI  epitopes, the second  variant  represented aTox and OprI  epitopes. Two variants  of ELISA  were developed to detect  the hybrid recombinant OprF-aTox-OprI protein. The first variant allows to determine the protein amount and to evaluate completeness of its adsorption on aluminum hydroxide. To confirm authenticity of the protein, both methods must be used, since they can detect all three antigens (OprF, aTox and OprI) which are present  in the fusion protein.
Polymorbidity, ageing of immune system and low-grade systemic inflammation: a challenge for modern medicine
Authors: Shirinsky V. S. , Shirinsky I. V.
Published: August 2020

The  review  article  considers the  data  from  literature that  concern polymorbidity aspects,  its interrelations with ageing of immune system and lo-grade immune ageing, mechanisms of genesis, approaches to its prevention and treatment. Evolution of “comorbidity” and “polymorbidity” terms is traced, an updated definition of polymorbidity is proposed. The  world-wide incidence of polymorbidity is increased and  now it reaches  23-25%  in general  population, and up to 98%, in elderly people  (> 65 years old).  The risk factors  of polymorbidity are considered, like as its social burden due to high costs for healthcare, high mortality rates, excessive treatment provided by multidisciplinary specialists.  We present  evidence  for common molecular and cellular  mechanisms involved  in ageing and polymorbidity, being unified  by the term  “inflammaging” which represents a low-grade chronic systemic  inflammation associated with  ageing.  The  data  are  presented that concern the “inflammaging” development with involvement of ageing cells from innate and adaptive immunity systems,  different   pro and  anti-inflammatory mediators, lifelong  antigenic load.  The  data  are  analyzed concerning functional and  structural changes  in the  inborn and  adaptive  immune system  in ageing,  role of these changes  in “inflammaging” persistence and development of polymorbid conditions. There  are complex interactions shown between  the bodily senescence and immune ageing, with similar underlying mechanisms in some cases, however, being quite different  in other  instances. With age, upon  existing risk factors,  the changed adaptive  immunity in most people  is not able to full-scale  coping  with chronic antigenic load,  thus increasing the risk of diseases. Moreover, in many elderly people these changes are compensated by steady activation of the innate immunity cells. It is noted  that the aging events and development of disease (polymorbidity) cannot be considered distinct entities, since they can interact, being, however, basically different in their nature. In future, one should concentrate our efforts on elucidation of molecular and cellular mechanisms of these interactions, solution of the  tasks  oriented for development of such  interventions that  could  be able  to  reduce  harmful consequences of ageing and to use useful effects for health  maintenance and reaching maximal longevity.
Features of the endoscopic picture in paediatric gastroduodenal diseases caused by Helicobacter pylori
Authors: Turdieva Sh. T. , Shamansurova E. A.
Published: August 2020

The aim of the study was to examine the features of the endoscopic picture in the upper digestive tract mucosa in paediatric chronic gastroduodenal pathology (CGDP) associated with Helicobacter pylori. Materials and methods. There were examined 286 patients, aged 6–15 years. Diagnostic criteria for chronic gastroduodenal pathology were anamnestic as well as instrumental and functional studies data: gastric fractional intubation, esophagogastroduodenoscopy (EPGDS) with endoscopic pH-metry without biopsy, and ultrasound examination of abdominal organs. H. pylori testing was carried out by two unrelated methods such as respiratory test and a immunochromatographic fecal test. Results. Detection of H. pylori in children with CGDP peaked in patients with peptic gastric and duodenal ulcer (up to 87.5%, p 0.05). The main endoscopic signs were edema, hyperaemia and contact bleeding, as well as local haemorrhage were the major endoscopic signs of inflammation in the stomach and duodenum mucosa. Atrophic mucosal lesions were characterized by thinning, pale colour together with transilluminated submucosal vessels. Non-atrophic antral gastritis was featured with delayed gastric emptying, antral stasis and pyloric spasm. In contrast, hypotension of the gastric wall, duodenogastric reflux and decreased motility were more typical to chronic atrophic gastritis. Major endoscopic feature in patients with H. pylori infection was presented by dominant atrophic changes combined with gastroduodenal reflux (77.6%, p 0.05) compared to patients without H. pylori infection. Conclusion. Detection of HP infection was peaked in children with CGDP coupled to peptic ulcer disease compared to patients with inflammatory diseases (p 0.05). Endoscopic examination in HP-positive patients showed that atrophic changes were found by 4-fold more frequently together with gastroduodenal reflux compared to patients without HP infection (p 0.05).
Lessons to learn: COVID-19 epidemic in Italy
Authors: Semenov A. V. , Pshenichnaya N. Y.
Published: August 2020

Here we provide the assessment of COVID-19 epidemic in Italy, which scale has led to serious challenges both for society and national health care system. Despite timely information regarding the pandemic spread of the novel coronavirus infection, the country’s health care was not prepared to dramatically increased rate of patients with viral pneumonia at the first stage of the epidemic, infection control measures were not fully implemented that also led to spread of infection among health care workers. Socially vulnerable population groups did not seek timely medical care due to the lack of hospital facilities as well as well-trained medical personnel. At the second stage of developing epidemic, were also delayed and executed at varying timepoints in neighbor regions, with sustained insufficient management after implementing them that was reflected as ongoing rise of epidemic curve over long time. Delayed execution of anti-epidemic restrictive measures aimed at fighting against ongoing epidemic resulted in substantially increased morbidity and mortality among vulnerable population groups and retarded rate of decreasing COVID-19 epidemic curve. Analyzing response measures taken in Italy against COVID-19 epidemic should be appreciated by other countries while dealing with the current pandemic and preparing to react to novel biological threats in the future.
Some opportunities for immunotherapy in coronavirus infection
Authors: Smirnov V. S. , Totolian Areg A.
Published: August 2020

Here we review means of immunomodulatory therapy for coronavirus infection caused by SARS-CoV-2 (COVID-19). It has been appreciated that highly limited arsenal of relatively effective means and methods of prevention and treatment of the COVID-19 pandemic is available. The goal of our study was to analyze some therapeutic approaches based on available publications for COVID-19 treatment viewed from acting via innate immunity system. Convalescent plasma serotherapy represents one of the means with verified therapeutic efficacy that was accompanied with decreased viral load and relief of the disease symptoms. The drawback of serotherapy results from limited number of potential plasma donors and profound variety in amount of SARS-CoV-2-specific antibodies found in donor plasma. Another approach to COVID-19 therapy is based on using monoclonal antibodies engineered to target specific virus antigenic determinants, most often surface spike antigen. Antibodies blocking such antigen are able to prevent virus entrance into target cells and development of overt infection. On the other hand, there are monoclonal antibodies abrogating production or binding of excessive amounts of pro-inflammatory cytokines, such as IL-6, TNFα, etc., some of which (tocilizumab) have been already tested in COVID-19 therapy, whereas the remaining preparations are being currently investigated and tested. A certain breakthrough in COVID-19 therapy was provided by the well-known drugs chloroquine and dihydrochloroquine, which have proven to be effective as antiviral, anti-inflammatory and immunomodulatory means. Finally, a new multicomponent immunomodulatory preparation Cytovir-3 has been proposed already passed clinical trials and recommended for use in prevention and treatment of influenza and SARS and might have found its own niche in preventing COVID-19, as SARS-CoV-2 also belongs to the group of acute respiratory viruses. Thus, the arsenal of means for COVID-19 prevention and treatment contains the drugs for immunomodulatory therapy and prevention of immune-related disorders developing in response to invasion pathogenic viruses and lowering a risk of possible damage. Hence, correct and scientifically justified use of such remedies will increase overall effectiveness of fight against the coronavirus pandemic.
Quality of life and spirituality of patients with chronic kidney disease: pre- and post-transplant analysis
Authors: Olivera Letícia Meazzini de , Okuno Meiry Fernanda Pinto , Barbosa Dulce Aparecida , Sesso Ricardo de Castro Cintra , Scherrer Júnior Gerson , Pessoa João Luis Erbs , Fonseca Cassiane Dezoti da , Belasco Angélica Gonçalves Silva
Published: August 2020

ABSTRACT Objective: to compare the quality of life (QoL) of patients under dialysis and after kidney transplant; correlate the QoL of transplant patients to sociodemographic, morbid and spirituality/religiosity variables. Method: prospective study with a quantitative approach, with a sample of 27 patients who underwent peritoneal dialysis or dialysis undergoing kidney transplant. QoL and spirituality were assessed by the KDQOL-SF and WHOQOL-SRPB tools, being correlated with sociodemographic and economic variables. Results: the dimensions of total mental component, kidney disease effects and kidney disease burden showed significant improvement in the post-transplant period, with p <0.0004. There was a significant correlation between four dimensions of spirituality and seven dimensions of QoL; p ranged from 0.04 to 0.006. Conclusion: there was a significant improvement in QoL in the post-transplant period. The dimensions of spirituality: wholeness and integration, spiritual connection, wonder and inner peace were positively correlated with seven dimensions of QoL.
CARTOGRAFIA DA IMPLANTAÇÃO E EXECUÇÃO DO PROGRAMA SAÚDE NA ESCOLA (PSE): IMPLICAÇÕES PARA O PROCESSO DE DESMEDICALIZAÇÃO
Authors: BRAMBILLA DAIANE KUTSZEPA , KLEBA MARIA ELISABETH , MAGRO MÁRCIA LUÍZA PIT DAL
Published: August 2020

RESUMO: O presente artigo objetiva apresentar uma cartografia da implantação e execução do Programa Saúde na Escola (PSE) e seus efeitos para o processo de desmedicalização em um município de pequeno porte no sul do Brasil. Trata-se de um estudo cartográfico que contou com participação de 51 profissionais dos setores envolvidos no Programa. Para a produção dos dados foram utilizadas rodas de conversa, oficina de grupo, análise documental e observação participante. As análises indicam que as ações realizadas por meio do PSE explicitaram o processo de medicalização no cuidado a educandos, especialmente com foco na prescrição e uso de psicotrópicos. Por meio da articulação intersetorial promovida pelo Programa, aproximaram-se os setores Saúde, Educação e Assistência Social, ampliando-se a compreensão das problemáticas que envolviam os alunos, bem como o leque de ações promovidas para enfrenta-las. Isso indica o potencial do Programa para o processo de desmedicalização para o que a intersetorialidade mostrou-se determinante.
PROJETOS DE FORMAÇÃO HUMANA EUGENISTA E HIGIENISTA: PRESCRIÇÕES PARA A EDUCAÇÃO FÍSICA EM PERIÓDICOS (1932-1960)
Authors: CASSANI JULIANA MARTINS , FERREIRA NETO AMARÍLIO , SANTOS WAGNER DOS
Published: August 2020

RESUMO: O artigo objetiva compreender as orientações para o ensino da Educação Física, fundamentadas nos projetos de formação humana eugenista e higienista. Possui, como fontes, a Revista de Educação Física (230 matérias) e a Revista Educação Physica (405 matérias). Utiliza, como pressupostos teóricos, os conceitos de análise pela materialidade e práticas de apropriações. Os resultados sinalizam que o diálogo dos articulistas com os princípios da eugenia e do higienismo estava relacionado com os perfis editoriais das revistas, em um cenário de negociações em relação às perspectivas de formação assumidas por eles. Esse teorizar conferiu cientificidade à Educação Física e ofereceu as bases para a publicação de prescrições que orientassem a formação e a prática docente. Nas fontes, teoria e prática imbricavam-se, conformando as revistas como dispositivos de uso didático-pedagógico e contribuindo com a inserção e a consolidação da Educação Física nos currículos escolares.
METODOLOGIAS ATIVAS COMO SIGNIFICADO TRANSCENDENTAL DE CURRÍCULOS DE FORMAÇÃO MÉDICA
Authors: FRANÇA JUNIOR RAIMUNDO RODRIGUES DE , MAKNAMARA MARLÉCIO
Published: August 2020

RESUMO: O objetivo deste trabalho é mostrar como um currículo é significado pelas teorias pós-críticas e, a partir desse modo específico de interpretação, buscar problematizar os discursos que se sobressaem no currículo oficial médico de uma Instituição de Ensino Superior pública, que está baseado no uso das Metodologias Ativas. Para o alcance desse objetivo, fundamentamos nosso referencial nos estudos pós-críticos sobre currículo e analisamos documentos que o orientam. O exercício de problematização foi realizado mediante o emprego da análise de discurso de inspiração foucaultiana. O resultado alcançado foi a possibilidade de se enxergar o currículo analisado para além de um instrumento envolvido em um processo de ‘revelação’ de metodologias e de sujeitos considerados ideais. Significamos o(a) médico(a) como efeito da disputa entre diferentes discursos presentes nesse currículo e mostramos como esse(a) profissional é posicionado(a). Concluímos este trabalho explicitando pontos-chave de estudos sobre currículo na perspectiva pós-crítica, visando subsidiar futuras investigações.
POVOAMENTOS E RESISTÊNCIAS ENTRE DOCÊNCIA E CRIAÇÃO NO ENSINO DAS ARTES
Authors: MOSSI CRISTIAN POLETTI
Published: August 2020

RESUMO: O artigo surge como parte dos resultados, do aporte teórico e das ações desenvolvidas pela pesquisa intitulada Docência e criação em educação das artes visuais: povoamentos entre visualidades, leituras e escritas, coordenada pelo autor. Apresenta, assim, um arranjo elaborado com as noções presentes em seu título - especialmente criação, resistência/re-existência, povoamento e arte - para convidar o leitor a pensar concepções que envolvam uma possível perspectiva de formação de docências, a fim de atuar na educação das artes na contemporaneidade. Desse modo, o texto apresenta um modo de se relacionar com conceitos e imagens, tanto no que tange à própria investigação, quanto ao que se refere àquilo que acaba respingando no ambiente formativo de professores de artes visuais. No fim, apresenta e discute brevemente a proposta artístico-educacional Postais para o Futuro, idealizada e conduzida também pelo autor, como modo de evidenciar formas de ler e escrever com imagens em um horizonte que concebe palavras e visualidades como dispositivos de invenção, para além de anteparos de significação.
O GINÁSIO “INCONFIDÊNCIA” DE ALFENAS: VESTÍGIOS DA CULTURA ESCOLAR E DO ENSINO DE HISTÓRIA
Authors: SILVA GUSTAVO FELIPE COSTA E , FILGUEIRAS JULIANA MIRANDA , VIEIRA JULIANA SILVA
Published: August 2020

RESUMO: O artigo apresenta uma análise da criação do primeiro ginásio público da cidade de Alfenas e examina algumas práticas escolares e do ensino de História na escola. Por meio de documentos encontrados no arquivo morto da instituição e de entrevista com uma de suas professoras, foi possível identificar especificidades das práticas escolares que se configuraram no cotidiano da escola, situando-a num contexto nacional de intensas transformações educacionais que se deram entre os anos 1960 e 1990. O estudo revelou que as aulas de História se intercruzavam com as de Geografia, Educação Moral e Cívica e Organização Social e Política do Brasil. As experiências da professora entrevistada evidenciaram um movimento de acomodação e apropriação das prescrições, o que imprimia uma característica própria em seu fazer profissional. Os rituais constituídos pela escola colaboraram para sedimentar modos de agir e de pensar, introjetar comportamentos e hábitos. Contudo, as resistências e enfrentamentos dos pais, dos professores e, sobretudo, dos estudantes, indicaram tentativas de mudar os ritos e confrontar as regras escolares.
A TRANSMISSÃO DO CONHECIMENTO EM DEBATE: ESTARIA A PEDAGOGIA HISTÓRICO-CRÍTICA REABILITANDO O ENSINO TRADICIONAL?
Authors: PASQUALINI JULIANA CAMPREGHER , LAVOURA TIAGO NICOLA
Published: August 2020

RESUMO: O artigo se propõe a aprofundar o debate acerca da defesa da transmissão dos conhecimentos científicos, artísticos e filosóficos reivindicada pela pedagogia histórico-crítica no atual contexto político-cultural de obscurantismo e ataques à razão. Com base em investigação teórico-conceitual de tipo bibliográfica, explora as diferenças entre a proposição histórico-crítica e os métodos tradicionais de ensino, sustentando que o combate às pedagogias não-diretivas não outorga um movimento de reabilitação da pedagogia tradicional, marcada pela prevalência do método indutivo, pelo verbalismo e pela apreensão acrítica da relação escola-sociedade. Conclui-se que a pedagogia histórico-crítica conserva o princípio da transmissão do conhecimento para todos, mas, uma vez que baseia em outra concepção de conhecimento, realiza-se a partir de outro método e subordina-se a outra finalidade, tal princípio é requalificado, vislumbrando a práxis. Postula-se, enfim, a compreensão da transmissão de conhecimento como princípio de esfera ontológica - e não didática ou operacional - do processo educativo.
EDUCAR CORPOS, COSTUMES E SENSIBILIDADES: PRÁTICAS CULTURAIS ESTADUNIDENSES E INTERPRETAÇÕES SOBRE O BRASIL EM CIRCULAÇÃO PELA ASSOCIAÇÃO CRISTÃ DE MOÇOS
Authors: SILVA GIOVANNA CAMILA DA
Published: August 2020

RESUMO: A inauguração da Associação Cristã de Moços no Brasil e a expansão do seu projeto formativo contaram com um importante elemento atrativo: o protestantismo. Em finais do século XIX já circulavam no país ideias, proposições e práticas afinadas com a estrutura religiosa da Associação. Com a chegada de instituições e sujeitos estadunidenses no Brasil, aportavam no país formas de viver sintonizadas com padrões estadunidenses de operar no mundo. De forma sutil ou de modo explícito, é possível identificar elementos mobilizados também pela ACM para aproximação dos brasileiros com práticas culturais, com valores, com o modo de vida “americano”. Nas representações produzidas sobre o Brasil, os acemistas articularam um mecanismo que ora indicava as condições desfavoráveis do país, ora as tomava como mote para a ressonância das iniciativas da Associação. Indicar limitações, mas também apresentar alternativas para novos costumes, novas práticas, novas sensibilidades, essa parecia a dinâmica acemista.
Transmission of exchange rate variation to Brazilian pulp export prices
Authors: Copetti Leonardo Sangoi , Coronel Daniel Arruda
Published: August 2020

ABSTRACT: The aim of this research was to examine the relationship between exchange rate variations and Brazilian export prices for cellulose, which is defined as the exchange rate pass-through, taking the period from January 2000 to March 2019 as a reference. The data were collected on the websites of the Ministry of Development, Industry and Foreign Trade - MDIC, the Institute of Applied Economics - IPEA and the Federal Reserve Bank of St. Louis - FRED. Thus, time series instruments were used, especially the Error Correction Vector Model. Results provided indications that the pass-through degree of exchange rate for cellulose export prices occurred incompletely in the total period and in the second sub-period, and was null in the first sub-period, representing that depreciation of rate exchange rates did not translate into significant competitiveness gains, as they did not significantly reduced export prices.
Animalidad, comunidad e imaginarios de lo trans en la obra visual de Nicola Costantino
Authors: Francica Cynthia
Published: August 2020

Resumen: En el contexto actual de cuestionamiento radical de la categoría de lo ‘humano’, un sector de la producción visual contemporánea investiga los modos en que las subjetividades de género toman forma no sólo en relación con entornos culturales humanos, sino también a geografías materiales, naturales e inanimadas. Enfocándome en el caso de la artista argentina Nicola Costantino (Rosario, 1964), propongo que mediante la recuperación de superficies corporales sensuales, táctiles y afectivamente cargadas, su obra Peletería humana (1996-2003) negocia articulaciones hegemónicas de la sexualidad y el género e historias comunales de violencia, al tiempo que revisita imaginarios simbólicos nacionales para explorar y delimitar nuevas corporalidades y subjetividades potenciales en torno tanto a lo femenino como a lo queer y lo viviente.
Escribir la violencia hacia las mujeres. Feminismo, afectos y hospitalidad
Authors: Peller Mariela , Oberti Alejandra
Published: August 2020

Resumen: El artículo examina tres obras literarias contemporáneas argentinas que intervienen en las discusiones sobre el estatuto social de la violencia sexista y sus políticas de visibilización. Desde una perspectiva enmarcada en el movimiento feminista y sus redes afectivas, se analizan Beya (le viste la cara a Dios), de Gabriela Cabezón Cámara e Iñaki Echeverría (2013), Chicas muertas, de Selva Almada (2014) y Por qué volvías cada verano, de Belén López Peiró (2018). Son obras que abordan temas clásicos del feminismo -prostitución, femicidio y violación-, produciendo torsiones en los lugares sociales, en las posibilidades de toma de la palabra y en los archivos. No solo marcan la trama social que produce las violencias -y sus responsables- sino que también despliegan políticas de resistencia que permiten a las mujeres imaginarse más allá de victimizaciones en un colectivo amoroso.
MR. CARVALHO AND THE POST-KEYNESIANS: SCHOLAR, THEORIST, WRITER
Authors: Dymski Gary
Published: August 2020

ABSTRACT Fernando Cardim de Carvalho’s monumental intellectual legacy encompasses many elements - his teaching, his mentoring, his scholarship, and his policy activism. This essay explores Cardim’s contributions to Post-Keynesian economics through the lens of his 31 publications in refereed English-language journals. After applying some bibliometric analysis to these articles, we undertake a chronological review of the themes developed in Cardim’s publications on theoretical topics in Keynesian economics. This is followed by a clarification and defense of Cardim’s distinctive methodology. We then undertake a chronological review of the articles containing his critical analyses of development policy. This side-by-side comparison of Cardim’s theoretical and policy pre-occupations through time clarifies their close interconnection. We conclude by examining the craft of writing in Cardim’s work, as he was an economist whose impact stemmed not just what he said, but from how he said it. Seeing Fernando Cardim de Carvalho’s scholarship from these different angles - his multidimensional contributions as a scholar, as a theorist, a critic, and as a crafter of prose - permits a full appreciation of both the range and depth of his work as a Keynesian economist.
RACIONALIDADE E MÉTODO: DUAS CONTRIBUIÇÕES DE FERNANDO CARDIM DE CARVALHO À TEORIA PÓS-KEYNESIANA
Authors: Terra Fábio Henrique Bittes
Published: August 2020

RESUMO Em meio à vasta produção do Professor Cardim de Carvalho, encontram-se duas contribuições que ele ofereceu à teoria pós-keynesiana, quais sejam, as discussões sobre a racionalidade sob incerteza e método. Mais do que apenas dois conceitos estanques, Professor Cardim de Carvalho os relaciona ao debater o método de Keynes na Teoria Geral do Emprego, do Juro e da Moeda, argumentando que a compreensão da racionalidade humana coloca em contato o agente observador e o agente observado, fazendo com que o sujeito teórico apreenda o sujeito teorizado - o consumidor, o empresário, o especulador - não como um pressuposto, mas um dado da realidade. O que aproxima o analista do analisado é aquele compreender a racionalidade deste e isto é essencial para que Keynes, o sujeito, teorize sua economia monetária da produção, o objeto. Apresentar estas contribuições é o objetivo deste artigo.
CARDIM, KEYNES E MARX: UM DIÁLOGO INTERROMPIDO?
Authors: Corazza Gentil
Published: August 2020

RESUMO O objetivo deste texto é analisar o diálogo entre Keynes e Marx, em textos de Fernando Cardim de Carvalho. Embora o tema não seja novo, o diálogo tentado por Cardim apresenta particularidades. Ele se propõe analizar a teoria monetária de Marx a partir de uma perspectiva pós-keynesiana, mas outros temas também são objeto de comparação entre Marx e Keynes. A pergunta, que procuramos responder, é a seguinte: o diálogo buscado por Cardim teria sido realmente interrompido durante de seu mestrado ou estaria presente de forma implícita ao longo de sua carreira acadêmica? Buscando analisar essas questões, além da introdução e da conclusão, o texto aborda ainda os encontros e desencontros de Cardim com Marx, a teoria monetária de Marx, numa perspectiva pós-keynesiana e os pontos de convergência e divergência entre Marx e Keynes em textos de Fernando Cardim de Carvalho.
PREFERÊNCIA PELA LIQUIDEZ, FINANCEIRIZAÇÃO E EFEITOS DE PROPAGAÇÃO: DA NÃO NEUTRALIDADE DA MOEDA À NÃO NEUTRALIDADE DA FINANÇA
Authors: Herscovici Alain
Published: August 2020

RESUMO A crítica radical que Keynes faz à teoria dos fundos de empréstimos (TFE) revela a ruptura epistemológica entre a economia keynesiana e a economia neoclássica. Neste trabalho, estudarei a natureza dessa ruptura, mais especificamente no que diz respeito aos diferentes componentes da preferência pela liquidez e aos mecanismos a partir dos quais a incerteza se propaga nas diferentes esferas da economia. A partir dos trabalhos de Kahn e de Cardim de Carvalho, demonstrarei porque à não neutralidade da moeda é preciso acrescentar a não neutralidade do sistema financeiro. Na medida em que a atuação dos agentes se explica por meio de uma teoria da escolha dos ativos, mostrarei como a finança especulativa constitui um ativo específico e como ela exerce seus efeitos de propagação sobre a determinação da taxa de juros e, consequentemente, sobre o setor “real”. Em uma primeira parte, analisarei os fundamentos da crítica que Keynes faz à teoria dos fundos de empréstimos. Numa segunda parte, estudarei, a partir dos trabalhos de Cardim de Carvalho, as relações entre a preferência pela liquidez, a taxa de juros e a finança especulativa.