bluuebunny/crossref_metadata_2025 huggingface dataset:
Technique Advances in Enteroatmospheric Fistula Isolation After Open Abdomen: A Review and Outlook
Authors: Huang Jinjian , Ren Huajian , Jiang Yungang , Wu Xiuwen , Ren Jianan
Published: January 2021

Enteroatmospheric fistula (EAF) after open abdomen adds difficulties to the management and increases the morbidity and mortality of patients. As an effective measurement, reconstructing gastrointestinal tract integrity not only reduces digestive juice wasting and wound contamination, but also allows expedient restoration of enteral nutrition and intestinal homeostasis. In this review, we introduce several technologies for the temporary isolation of EAF, including negative pressure wound therapy, fistuloclysis, fistula patch, surgical covered stent, three-dimensional (3D) printing stent, and injection molding stent. The manufacture and implantation procedures of each technique with their pros and cons are described in detail. Moreover, the approach in combination with finger measurement, x-ray imaging, and computerized tomography is used to measure anatomic parameters of fistula and design appropriate 3D printer-recognizable stereolithography files for production of isolation devices. Given the active roles that engineers playing in the technology development, we call on the cooperation between clinicians and engineers and the organization of clinical trials on these techniques.
Smartband Use During Enhanced Recovery After Surgery Facilitates Inpatient Recuperation Following Minimally Invasive Colorectal Surgery
Authors: Yin Tzu-Chieh , Huang Ching-Wen , Tsai Hsiang-Lin , Su Wei-Chih , Ma Cheng-Jen , Chang Tsung-Kun , Wang Jaw-Yuan
Published: January 2021

Background: Enhanced recovery after surgery (ERAS) is valuable in perioperative care for its ability to improve short-term surgical outcomes and facilitate patient recuperation after major surgery. Early postoperative mobilization is a vital component of the integrated care pathway and is a factor strongly associated with successful outcomes. However, early mobilization still has various definitions and lacks specific strategies.

Methods: Patients who underwent minimally invasive surgery for colorectal cancer followed our perioperative ERAS program, including mobilization from the first postoperative day. After perioperative care skills were improved in our well-established program, compliance, inpatient surgical outcomes, and complications associated with adding smartband use were evaluated and compared with the outcomes for standard protocol. Quality of recovery was evaluated using patient-rated QoR-40 questionnaires the day before surgery, on postoperative days 1 and 3, and on the day of discharge.

Results: Smartband use after minimally invasive colorectal surgery failed to increase compliance with early mobilization or reduce the occurrence of postoperative complications significantly compared with standard ERAS protocol. However, when smartbands were utilized, quality of recovery was optimized and patients returned to their preoperative status earlier, at postoperative day 3. The length of hospital stay, as defined by discharge criteria, and hospital stay of patients without complications was reduced by 1.1 and 0.9 days, respectively (P = 0.009 and 0.049, respectively).

Conclusions: Smartbands enable enhanced communication between patients and surgical teams and strengthen self-management in patients undergoing minimally invasive colorectal resection surgery. Accelerated recovery to preoperative functional status can be facilitated by integrating smartbands into the process of early mobilization during ERAS.
Modeling Thermal Suitability for Reindeer (Rangifer tarandus ssp.) Brainworm (Elaphostrongylus rangiferi) Transmission in Fennoscandia
Authors: Rose Vineer Hannah , Mørk Torill , Williams Diana J. , Davidson Rebecca K.
Published: January 2021

The brainworm, Elaphostrongylus rangiferi, is a nematode which causes neurological disorders (elaphostrongylosis) in reindeer (Rangifer tarandusssp.). Favorable climatic conditions have been inferred as the cause of sporadic outbreaks of elaphostrongylosis in Norway, supported by positive associations between observed outbreaks/intensity of infection and summer temperatures in the previous years. Climate warming which results in increased transmission ofE. rangiferitherefore presents a risk to the health of semi-domesticated and wild reindeer in Fennoscandia (Norway, Sweden, and Finland), the health of co-grazing small ruminants, and the livelihoods of indigenous Sámi herders. As a first step toward developing climate change impact assessments forE. rangiferi, a degree-day model was developed for larval development in a range of gastropod hosts and applied to historic weather data. Predictions were validated by statistical and qualitative comparison against historic parasitological and outbreak records. The model predicted an overall increase in thermal suitability forE. rangiferi, which was statistically significant in the north and along the Scandinavian mountain ranges, where reindeer density is highest. In these regions annual cumulative temperature conditions are suitable for larval development within a single year, potentially changingE. rangiferiepidemiology from a 2-year transmission cycle to a 1-year transmission cycle. This is the first mechanistic model developed forE. rangiferiand could be used to inform veterinary risk assessments on a broad spatial scale. Limitations and further developments are discussed.
Environmental Recovery of Nosocomial Bacteria in a Companion Animal Shelter Before and After Infection Control Procedures
Authors: Horsman Sara , Rynhoud Hester , Zhou Xiaoyan , Soares Magalhães Ricardo J. , Gibson Justine S. , Meler Erika
Published: January 2021

While the effects of cleaning and disinfection practices on the reduction of environmental nosocomial bacteria are well-established in human and large animal veterinary hospitals, how animal movements within animal health care facilities influence environmental bacterial recovery is poorly understood. During three consecutive weeks, 155 electrostatic wipes were collected from the environment pre- and post-cleaning only or following disinfection from seven target locations within an animal shelter. All samples were cultured, and isolates were identified using a matrix-assisted laser desorption ionization—time of flight mass spectrometry. Social network analysis of animal movements during the sampling period was performed to estimate the level of connectivity of the seven target locations. The relationship between bacterial levels and connectivity estimates of the target locations were investigated using a negative binomial regression model with a random effect of sampling areas. Overall, our results indicate a significant reduction in the total bacterial contamination with disinfection when compared to cleaning only [Coefficient (Coef.) = −1.72, 95% Confidence Interval (CI) = −3.09, −0.34, P = 0.015]. Higher total bacterial contamination was significantly more likely in sampled areas with less animal movement connectivity (Coef. = −0.32, 95% CI = −0.49, −0.15, P ≤ 0.001). Pseudomonas aeruginosa and ampicillin resistant Enterobacteriaceae (Escherichia coli, Enterobacter spp. and Klebsiella spp.) were present in the animal holdings and in the shelters' veterinary clinic environment at all sampling times. Our findings demonstrate that cleaning followed by disinfection practices are effective at reducing environmental bacterial levels. Areas with less animal connectivity are more likely to have a higher bacterial contamination. These areas could represent environmental reservoirs for bacterial infection and should be targeted with effective cleaning and disinfection protocols.
Acknowledgment to Reviewers of Actuators in 2020
Authors:   Actuators Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Actuators maintains its standards for the high quality of its published papers [...]
Panicle Nitrogen Strategies for Nitrogen-Efficient Rice Varieties at a Moderate Nitrogen Application Rate in the Lower Reaches of the Yangtze River, China
Authors: Ju Chengxin , Liu Tao , Sun Chengming
Published: January 2021

Nitrogen (N) management is of great importance in rice production, but most previous studies have focused on high N rates and there is a lack of research on management plans under a moderate N rate. This study aimed to explore the agronomic and physiological traits of N-efficient rice varieties (NEVs) and to optimize the management strategy at an N rate below the inflection point of the parabolic curve between N rate and grain yield. Two NEVs and two N-inefficient rice varieties (NIVs) were planted, and three treatments were designed according to the panicle N application method. A larger amount of N applied at panicle initiation (PI) led to higher rice yield and N-use efficiency (NUE). This was mainly due to increases in the total number of spikelets per unit area, root oxidation activity, leaf area duration, and leaf photosynthesis rate as well as to the increased carbon (C) and N utilization rates. Compared with NIVs, NEVs exhibited improved root and shoot functions and higher C and N transport characteristics at the moderate N rate. We suggest that increasing the application of N at PI and that planting of NEVs are important ways to increase rice yield and NUE when adopting moderate N rates.
Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
Authors: Viejobueno Josefina , Albornoz Patricia Liliana , Camacho María , de los Santos Berta , Martínez-Zamora Martín Gustavo , Salazar Sergio Miguel
Published: January 2021

Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.
Divergent Seasonal Reproductive Patterns in Syntopic Populations of Two Murine Species in Southern Spain, Mus spretus and Apodemus sylvaticus
Authors: Massoud Diaa , Lao-Pérez Miguel , Ortega Esperanza , Burgos Miguel , Jiménez Rafael , Barrionuevo Francisco J.
Published: January 2021

In most mammals with seasonal reproduction, males undergo testis regression during the non-breeding period. We performed a morphological, hormonal, functional, and molecular study of the testes of sexually inactive males of two species of murine rodents, the wood mouse, Apodemus sylvaticus, and the Algerian mouse, Mus spretus, in syntopic populations of southern Iberian peninsula. Both species reproduce during most of the year, but wood mice stop breeding in the summer whereas Algerian mice do it in winter. Sexually inactive males of A. sylvaticus show complete testis regression with reduced levels of serum testosterone and abnormal distribution of cell-adhesion molecules. Contrarily, inactive males of M. spretus maintain almost normal spermotogenesis despite a significant reduction of androgenic function. The lack of an evident explanation for the divergent seasonal breeding patterns found in southern populations of A. sylvaticus and M. spretus, compared with northern ones, implies that very subtle species/population-specific features and/or non-conspicuous environmental cues probably operate to determine their seasonal breeding pattern. These results also support the notion that multiple models of circannual testis variation are possible for different populations of the same species, showing that the mechanisms controlling seasonal reproduction are in fact very plastic and fast evolving.
Impact of Dietary Supplementation with Moringa oleifera Leaves on Performance, Meat Characteristics, Oxidative Stability, and Fatty Acid Profile in Growing Rabbits
Authors: Selim Shaimaa , Seleiman Mahmoud , Hassan Mohamed , Saleh Ahmed , Mousa Mohamed
Published: January 2021

Moringa oleifera leaves (MOL) have gained great interest as a non-traditional feed ingredient due to their unique nutritional value. Therefore, the objective of the current study was to evaluate the effects of graded dietary supplementation levels with MOL on performance, carcass characteristics, antioxidant capacity, blood biochemical constituents, meat quality, and fatty acids profile of growing rabbits. A total of 120 weaned New Zealand white rabbits (6 weeks old) were randomly allotted into 4 dietary groups with 5 replicates each (n = 6), which were fed for 42 days with a basal diet as control or 3 experimental diets supplemented with 5, 10, or 15 g/kg MOL. The results showed that, compared to the control group, the dietary inclusion of MOL at a level of 10 and 15 g/kg DM linearly increased (p < 0.01) final live weight (2403.3 and 2498.2 vs. 2166.6) and average daily weight gain (36.5 and 35.51 g/d vs. 28.72 g/d), and enhanced feed conversion ratio (2.49 and 2.50 vs. 3.14). The dietary supplementation with MOL linearly increased dressing out percentage, spleen index, intestinal length, and decreased abdominal fat index (p < 0.01). Greater serum levels of total protein and globulin, but lower alanine aminotransferase and aspartate aminotransferase were observed in the MOL-fed rabbits (p < 0.01). Serum levels of total triglycerides, cholesterol, and low-density lipoprotein (p < 0.05) were decreased linearly and quadratically in the MOL groups compared with the control. Glutathione peroxidase activity increased (p < 0.01), whereas malondialdehyde decreased (p < 0.01) linearly and quadratically in both serum and meat, in response to dietary MOL supplementation. Dietary MOL supplementation increased the meat crude protein content but lowered the relative content of ether extract in the meat (p < 0.05). The relative content of the meat n-3 PUFA was increased by about 33.71%, 29.46%, and 24.36% for the MOL0.5%, MOL1%, and MOL1.5% groups compared to control. In conclusion, MOL could be used at a level of 1.5g/kg of the growing rabbits’ diets with beneficial impacts on performance, antioxidant capacity, and the nutritional value of the meat.
Convergent Evolution of Himalayan Marmot with Some High-Altitude Animals through ND3 Protein
Authors: Bao Ziqiang , Li Cheng , Guo Cheng , Xiang Zuofu
Published: January 2021

The Himalayan marmot (Marmota himalayana) mainly lives on the Qinghai-Tibet Plateau and it adopts multiple strategies to adapt to high-altitude environments. According to the principle of convergent evolution as expressed in genes and traits, the Himalayan marmot might display similar changes to other local species at the molecular level. In this study, we obtained high-quality sequences of the CYTB gene, CYTB protein, ND3 gene, and ND3 protein of representative species (n = 20) from NCBI, and divided them into the marmot group (n = 11), the plateau group (n = 8), and the Himalayan marmot (n = 1). To explore whether plateau species have convergent evolution on the microscale level, we built a phylogenetic tree, calculated genetic distance, and analyzed the conservation and space structure of Himalayan marmot ND3 protein. The marmot group and Himalayan marmots were in the same branch of the phylogenetic tree for the CYTB gene and CYTB protein, and mean genetic distance was 0.106 and 0.055, respectively, which was significantly lower than the plateau group. However, the plateau group and the Himalayan marmot were in the same branch of the phylogenetic tree, and the genetic distance was only 10% of the marmot group for the ND3 protein, except Marmota flaviventris. In addition, some sites of the ND3 amino acid sequence of Himalayan marmots were conserved from the plateau group, but not the marmot group. This could lead to different structures and functional diversifications. These findings indicate that Himalayan marmots have adapted to the plateau environment partly through convergent evolution of the ND3 protein with other plateau animals, however, this protein is not the only strategy to adapt to high altitudes, as there may have other methods to adapt to this environment.
Incorporation of Hybrid Nanomaterial in Dental Porcelains: Antimicrobial, Chemical, and Mechanical Properties
Authors: Vidal Carla L. , Ferreira Izabela , Ferreira Paulo S. , Valente Mariana L. C. , Teixeira Ana B. V. , Reis Andréa C.
Published: January 2021

Biofilm formation on biomaterials is a challenge in the health area. Antimicrobial substances based on nanomaterials have been proposed to solve this problem. The aim was to incorporate nanostructured silver vanadate decorated with silver nanoparticles (β-AgVO3) into dental porcelains (IPS Inline and Ex-3 Noritake), at concentrations of 2.5% and 5%, and evaluate the surface characteristics (by SEM/EDS), antimicrobial activity (against Streptococcus mutans, Streptococcus sobrinus, Aggregatibacter actinomycetemcomitans, and Pseudomonas aeruginosa), silver (Ag+) and vanadium (V4+/V5+) ions release, and mechanical properties (microhardness, roughness, and fracture toughness). The β-AgVO3 incorporation did not alter the porcelain’s components, reduced the S. mutans, S. sobrinus and A. actinomycetemcomitans viability, increased the fracture toughness of IPS Inline, the roughness for all groups, and did not affect the microhardness of the 5% group. Among all groups, IPS Inline 5% released more Ag+, and Ex-3 Noritake 2.5% released more V4+/V5+. It was concluded that the incorporation of β-AgVO3 into dental porcelains promoted antimicrobial activity against S. mutans, S. sobrinus, and A. actinomycetemcomitans (preventing biofilm formation), caused a higher release of vanadium than silver ions, and an adequate mechanical behavior was observed. However, the incorporation of β-AgVO3 did not reduce P. aeruginosa viability and increased the surface roughness of dental porcelains.
Revealing Social Media Phenomenon in Time of COVID-19 Pandemic for Boosting Start-Up Businesses through Digital Ecosystem
Authors: Susanto Heru , Fang Yie Leu , Mohiddin Fadzliwati , Rahman Setiawan Arief Amier , Haghi Parastou Khodaparast , Setiana Desi
Published: January 2021

When the world was engulfed by a COVID-19 pandemic crisis, various activities could not be carried out normally. Activities may continue from home during a crisis by the use of a smartphone through the internet, because almost all people have their own smartphone, without requiring additional purchase of hardware. For formal learning, people can take the opportunity to use social media platforms to undertake conversations easily by using smart phones, even tablets. Teenagers or adults who are still studying in higher education institutions can undertake continuous learning for their work assignments. For example, they can use these tools to communicate through social media such as the WhatsApp, Telegram, Zoom, Microsoft Team, and Edmodo apps to connect with friends and lecturers. This research reveals trends of digital social media security and usability in the time of the COVID-19 pandemic situation, such as working from home, establishing a start-up, improving business processes and conducting online business within the digital ecosystem. The result is interesting and there are many uses of social media which have been addressed in this study during the COVID-19 pandemic for business purposes. The various social media platforms have different features that are available for use by subscribers, and also make it easier for people to do business. This is mainly due to the fact that they open up the global market and also make it cheaper to advertise. The government as well as the private sector has been in the forefront when it comes to the use of social media. Maintaining a good online presence is one of the key aspects that determine the success of start-up companies. This is due to the fact that most customers usually rely on the customer reviews in determining the ability of a company to meet the needs of clients. The main reason why most companies set up a customer relations department that is mandated with the responsibility of responding to customer feedback on various online platforms. On the other hand, the increased use of social media has brought new challenges when it comes to the security of information. Users must, therefore, secure their servers and technology from external and internal threats. One of the strategies used is the use of passwords to log into a portal where each person authorized to access the portal is provided with a password that is unique and known only by the user. The study has covered all these areas in detail including the use of database management systems in an organization or individual.
Valley Winds at the Local Scale: Correcting Routine Weather Forecast Using Artificial Neural Networks
Authors: Dupuy Florian , Duine Gert-Jan , Durand Pierre , Hedde Thierry , Pardyjak Eric , Roubin Pierre
Published: January 2021

In regions of complex topography, local flows are difficult to forecast on a routine basis, especially in stable conditions, due to the coarse resolution of operational models. The Cadarache valley (southeastern France) features this sort of complex topography. The Weather Research and Forecasting (WRF) model is run daily to forecast the weather in this region with a horizontal resolution of 3 km. Such a resolution cannot resolve all topography details of the small Cadarache valley, and therefore its local wind patterns. Other variables, however, that are less dependent on the subgrid topography, are satisfactorily forecasted, and used as inputs to an artificial neural network (ANN) designed to reproduce wind observations inside the valley from WRF forecasts. A variable selection procedure identified 5 key input variables that best drive the ANN. With respect to the WRF output, the ANN significantly improves forecasted low-level winds, both for speed and direction. This study demonstrates the potential for the ANN technique to be used as a correcting tool to forecast weather conditions at the local scale when numerical modeling is performed at a resolution too coarse to take into account the effect of local topography.
Different Relationships between Arctic Oscillation and Ozone in the Stratosphere over the Arctic in January and February
Authors: Liu Meichen , Hu Dingzhu
Published: January 2021

We compare the relationship between the Arctic Oscillation (AO) and ozone concentration in the lower stratosphere over the Arctic during 1980–1994 (P1) and 2007–2019 (P2) in January and February using reanalysis datasets. The out-of-phase relationship between the AO and ozone in the lower stratosphere is significant in January during P1 and February during P2, but it is insignificant in January during P2 and February during P1. The variable links between the AO and ozone in the lower stratosphere over the Arctic in January and February are not caused by changes in the spatial pattern of AO but are related to the anomalies in the planetary wave propagation between the troposphere and stratosphere. The upward propagation of the planetary wave in the stratosphere related to the positive phase of AO significantly weakens in January during P1 and in February during P2, which may be related to negative buoyancy frequency anomalies over the Arctic. When the AO is in the positive phase, the anomalies of planetary wave further contribute to the negative ozone anomalies via weakening the Brewer–Dobson circulation and decreasing the temperature in the lower stratosphere over the Arctic in January during P1 and in February during P2.
Acknowledgment to Reviewers of Bioengineering in 2020
Authors:   Bioengineering Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Bioengineering maintains its standards for the high quality of its published papers [...]
Cell Proliferation Is Strongly Associated with the Treatment Conditions of an ER Stress Inducer New Anti-Melanoma Drug in Melanoma Cell Lines
Authors: Szász István , Koroknai Viktória , Patel Vikas , Hajdú Tibor , Kiss Tímea , Ádány Róza , Balázs Margit
Published: January 2021

HA15 is a new anti-melanoma drug that triggers endoplasmic reticulum (ER) stress and causes deleterious effects on melanoma cell viability due to autophagy and apoptosis, regardless of driver mutations or drug resistance. In this study, we investigated the effect of HA15 on the viability/proliferation of BRAFV600E-mutant melanoma cells using different culture conditions. In contrast to the published data, we did not detect significant melanoma cell death under normal culture conditions using HA15 treatment. Indeed, only cells that were cultured under long-term starvation conditions were sensitive to the drug. Quantitative measurements of ER stress and autophagy markers showed that the compound HA15 does not trigger stress alone but synergistically enhances ER stress under starvation conditions. Importantly, we observed that the viability of normal melanocytes decreased significantly with treatment, even at low HA15 concentrations. Finally yet importantly, we were able to generate HA15-resistant cell lines, which failed by Cerezo et al. In summary, HA15 only influences the viability of cells that are starved for several hours before and during treatment. However, this in vitro setting is far from the in vivo conditions. In addition, our data clearly show that melanoma cells can acquire HA15 resistance. Further studies are needed to prove that HA15 is an effective anti-cancer agent.
The Sarcoma Immune Landscape: Emerging Challenges, Prognostic Significance and Prospective Impact for Immunotherapy Approaches
Authors: Koumarianou Anna , Duran-Moreno Jose
Published: January 2021

Despite significant advances in multidisciplinary treatment strategies including surgery, radiotherapy, targeted therapy and chemotherapy there are yet no substantial improvements in the clinical benefit of patients with sarcomas. Current understanding of the underlying cellular and molecular pathways which govern the dynamic interactions between the tumor stroma, tumor cells and immune infiltrates in sarcoma tissues, led to the clinical development of new therapeutic options based on immunotherapies. Moreover, progress of the treatment of sarcomas also depends on the identification of biomarkers with prognostic and predictive values for selecting patients most likely to benefit from these new therapeutic treatments and also serving as potent therapeutic targets. Novel combinations with radiotherapy, chemotherapy, targeted therapy, vaccines, CAR-T cells and treatments targeting other immune components of the tumor microenvironment are underway aiming to bypass known resistance mechanisms. This review focuses on the role of tumor microenvironment in sarcoma, prognosis and response to novel immunotherapies.
Decision-making in Surgery or Active Surveillance for Low Risk Papillary Thyroid Cancer During the COVID-19 Pandemic
Authors: Sawka Anna , Ghai Sangeet , Ihekire Ogemdi , Jones Jennifer , Gafni Amiram , Baxter Nancy , Goldstein David ,   on behalf of the Canadian Thyroid Cancer Active Surveillance Study Group
Published: January 2021

We describe our experience conducting a prospective observational cohort study on the management of small, low risk papillary thyroid cancer during the COVID-19 pandemic. Our study participants are given the choice of active surveillance (AS) or surgery, and those in the AS arm are followed at the study center, whereas surgical patients undergo usual care. During the pandemic we have transitioned from in-person research patient visits to largely virtual care of patients under AS. As of 30 October 2020, we had enrolled 181 patients enrolled in our study (including 25 during the pandemic), of which 92.3% (167/181) consented to telephone communication and 79.0% (143/181) consented to secure videoconferencing communication. Prior to the pandemic, 74.5% (117/157) of our patients chose AS over surgery, whereas during the pandemic, 96.0% (24/25) chose AS. Of the 133 study patients who were under AS within the timeframe from 12 March 2020, to 30 October 2020, the percentage of patients who missed appointments was 8.3% (11/133, for neck ultrasound and physician visits, respectively) and delayed appointments was 23.3% (31/133). This preliminary data suggests that prospective observational research on AS of thyroid cancer can safely continue during the pandemic.
High-Content Analysis-Based Sensitivity Prediction and Novel Therapeutics Screening for c-Met-Addicted Glioblastoma
Authors: Oh Jeong-Woo , Oh Yun Jeong , Han Suji , Her Nam-Gu , Nam Do-Hyun
Published: January 2021

(1) Background: Recent advances in precision oncology research rely on indicating specific genetic alterations associated with treatment sensitivity. Developing ex vivo systems to identify cancer patients who will respond to a specific drug remains important. (2) Methods: cells from 12 patients with glioblastoma were isolated, cultured, and subjected to high-content screening. Multi-parameter analyses assessed the c-Met level, cell viability, apoptosis, cell motility, and migration. A drug repurposing screen and large-scale drug sensitivity screening data across 59 cancer cell lines and patient-derived cells were obtained from 125 glioblastoma samples. (3) Results: High-content analysis of patient-derived cells provided robust and accurate drug responses to c-Met-targeted agents. Only the cells of one glioblastoma patient (PDC6) showed elevated c-Met level and high susceptibility to the c-Met inhibitors. Multi-parameter image analysis also reflected a decreased c-Met expression and reduced cell growth and motility by a c-Met-targeting antibody. In addition, a drug repurposing screen identified Abemaciclib as a distinct CDK4/6 inhibitor with a potent c-Met-inhibitory function. Consistent with this, we present large-scale drug sensitivity screening data showing that the Abemaciclib response correlates with the response to c-Met inhibitors. (4) Conclusions: Our study provides a new insight into high-content screening platforms supporting drug sensitivity prediction and novel therapeutics screening.
Prognostic Value of Plasma hPG80 (Circulating Progastrin) in Metastatic Renal Cell Carcinoma
Authors: Kohli Manish , Tan Winston , Vire Bérengère , Liaud Pierre , Blairvacq Mélina , Berthier Frederic , Rouison Daniel , Garnier George , Payen Léa , Cousin Thierry , Joubert Dominique , Prieur Alexandre
Published: January 2021

Precise management of kidney cancer requires the identification of prognostic factors. hPG80 (circulating progastrin) is a tumor promoting peptide present in the blood of patients with various cancers, including renal cell carcinoma (RCC). In this study, we evaluated the prognostic value of plasma hPG80 in 143 prospectively collected patients with metastatic RCC (mRCC). The prognostic impact of hPG80 levels on overall survival (OS) in mRCC patients after controlling for hPG80 levels in non-cancer age matched controls was determined and compared to the International Metastatic Database Consortium (IMDC) risk model (good, intermediate, poor). ROC curves were used to evaluate the diagnostic accuracy of hPG80 using the area under the curve (AUC). Our results showed that plasma hPG80 was detected in 94% of mRCC patients. hPG80 levels displayed high predictive accuracy with an AUC of 0.93 and 0.84 when compared to 18–25 year old controls and 50–80 year old controls, respectively. mRCC patients with high hPG80 levels (>4.5 pM) had significantly lower OS compared to patients with low hPG80 levels (<4.5 pM) (12 versus 31.2 months, respectively; p = 0.0031). Adding hPG80 levels (score of 1 for patients having hPG80 levels > 4.5 pM) to the six variables of the IMDC risk model showed a greater and significant difference in OS between the newly defined good-, intermediate- and poor-risk groups (p = 0.0003 compared to p = 0.0076). Finally, when patients with IMDC intermediate-risk group were further divided into two groups based on hPG80 levels within these subgroups, increased OS were observed in patients with low hPG80 levels (<4.5 pM). In conclusion, our data suggest that hPG80 could be used for prognosticating survival in mRCC alone or integrated to the IMDC score (by adding a variable to the IMDC score or by substratifying the IMDC risk groups), be a prognostic biomarker in mRCC patients.
A Process Evaluation of A UK Classroom-Based Physical Activity Intervention—‘Busy Brain Breaks’
Authors: Cline Alice , Knox Gareth , De Martin Silva Luciana , Draper Stephen
Published: January 2021

The gap between development of effective physical activity interventions and the wide-scale adoption of these interventions in real-world settings has been reported since the early 2000s. Evaluations have been criticised for failing to report details of context, implementation, adoption and maintenance. ‘Busy Brain Breaks’ was an intervention designed to improve fundamental movement patterns whilst increasing physical activity within the classroom. This evaluation study used a mixed-methods approach including questionnaires, observations, semi-structured interviews and quantification of class-level dose. Findings suggest that giving teachers flexibility and autonomy over the way in which they implement physical activity interventions may increase the likelihood of adoption. Time was frequently perceived as a significant barrier to the intervention, giving the teachers flexibility to implement the intervention when they thought most suitable allowed teaching staff to retain their autonomy and make the intervention work with their schedule. Children’s behaviour appeared to be both a facilitator and barrier to implementing physical activity interventions within the classroom. Whilst misbehaviour can pose as a barrier, children’s enjoyment acts as a key facilitator to implementation for teaching practitioners. Teachers interviewed (n = 17) observed that movement ability had developed as a result of the intervention and recognised co-ordination, balance and stability as areas that had noticeably improved. Conducting an in-depth process evaluation has allowed for greater insight and understanding as to how, and to what extent, the intervention was implemented within the school-based setting.
Update on the Role of High-Flow Nasal Cannula in Infants with Bronchiolitis
Authors: Fainardi Valentina , Abelli Lara , Muscarà Maria , Pisi Giovanna , Principi Nicola , Esposito Susanna
Published: January 2021

Bronchiolitis (BR), a lower respiratory tract infection mainly caused by respiratory syncytial virus (RSV), can be very severe. Presently, adequate nutritional support and oxygen therapy remain the only interventions recommended to treat patients with BR. For years, mild BR cases were treated with noninvasive standard oxygen therapy (SOT), i.e., with cold and poorly or totally non-humidified oxygen delivered by an ambient headbox or low-flow nasal cannula. Children with severe disease were intubated and treated with invasive mechanical ventilation (IMV). To improve SOT and overcome the disadvantages of IMV, new measures of noninvasive and more efficient oxygen administration have been studied. Bi-level positive air way pressure (BiPAP), continuous positive airway pressure (CPAP), and high-flow nasal cannula (HFNC) are among them. For its simplicity, good tolerability and safety, and the good results reported in clinical studies, HFNC has become increasingly popular and is now widely used. However, consistent guidelines for initiation and discontinuation of HFNC are lacking. In this narrative review, the role of HFNC to treat infants with BR is discussed. An analysis of the literature showed that, despite its widespread use, the role of HFNC in preventing respiratory failure in children with BR is not precisely defined. It is not established whether it can offer greater benefits compared to SOT and when and in which infants it can replace CPAP or BiPAP. The analysis of the results clearly indicates the need for multicenter studies and official guidelines. In the meantime, HFNC can be considered a safe and effective method to treat children with mild to moderate BR who do not respond to SOT.
A Survey Instrument to Measure Skeptics’ (Dis)Trust in Climate Science
Authors: Sarathchandra Dilshani , Haltinner Kristin
Published: January 2021

Existing survey instruments of trust in science and scientists that focus on the general public are potentially insufficient to assess climate skeptics’ perspectives towards climate science. They may miss important aspects of climate science about which skeptics raise concerns, and may not accurately measure climate skeptics’ distrust in climatology. We introduce a new survey instrument developed using data gathered from interviewing 33 self-identified climate change skeptics in Idaho. The survey items capture skeptics’ beliefs regarding climate scientists’ trustworthiness and credibility, their deference to scientific authority, and their perceptions of alienation from the climate science community. We validate our survey instrument using data from an online survey administered to 1000 residents in the U.S. Pacific Northwest who are skeptical of climate change. By employing standard survey design principles, we demonstrate how our new (dis)trust in climate science instrument performs in tandem with well-known predictors of science attitudes and pro-environmentalism.
Acknowledgment to Reviewers of Diagnostics in 2020
Authors:   Diagnostics Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Diagnostics maintains its standards for the high quality of its published papers [...]
Pre-Clerkship Medical Students’ Experiences and Perspectives of System 1 and System 2 Thinking: A Qualitative Study
Authors: Sanders William , McHugh Douglas
Published: January 2021

Dual-process theories may be invoked to explain how physicians interact with, interpret, and draw inferences from clinical information. Stanovich and West’s model articulates two kinds of thinking—intuitive-based System 1 and analytical-based System 2—which have been under-investigated with physicians in training. This qualitative study explored pre-clerkship medical students’ retrospective perspectives and experiences of System 1 and System 2 thinking via 12 semi-structured interviews and abductive, progressive focusing. Participants identified patient interactions, clinical note writing, knowledge synthesis, problem list and differential diagnosis generation, evaluating evidence, and critical appraisal of literature as pre-clerkship opportunities to engage in System 1 or System 2 thinking. Six major themes emerged from analysis of participants’ interview transcripts: cognitive processes, deliberate practice, learning environment: being alone or being together, stickiness factor, biases and heuristics, and prior experience of attaining competence. Participants valued the anticipated role that System 1 and System 2 thinking will play in their future practice, and experienced nascent, self-regulated development of these cognitive processes during the pre-clerkship phase of their education without formal feedback or coaching from clinician preceptors. Pre-clerkship curricula should further embrace low-stakes, incremental teaching on metacognition and continuous monitoring of knowledge processing as a key competency for physician learners.
Real-Time Autonomous Residential Demand Response Management Based on Twin Delayed Deep Deterministic Policy Gradient Learning
Authors: Ye Yujian , Qiu Dawei , Wang Huiyu , Tang Yi , Strbac Goran
Published: January 2021

With the roll-out of smart meters and the increasing prevalence of distributed energy resources (DERs) at the residential level, end-users rely on home energy management systems (HEMSs) that can harness real-time data and employ artificial intelligence techniques to optimally manage the operation of different DERs, which are targeted toward minimizing the end-user’s energy bill. In this respect, the performance of the conventional model-based demand response (DR) management approach may deteriorate due to the inaccuracy of the employed DER operating models and the probabilistic modeling of uncertain parameters. To overcome the above drawbacks, this paper develops a novel real-time DR management strategy for a residential household based on the twin delayed deep deterministic policy gradient (TD3) learning approach. This approach is model-free, and thus does not rely on knowledge of the distribution of uncertainties or the operating models and parameters of the DERs. It also enables learning of neural-network-based and fine-grained DR management policies in a multi-dimensional action space by exploiting high-dimensional sensory data that encapsulate the uncertainties associated with the renewable generation, appliances’ operating states, utility prices, and outdoor temperature. The proposed method is applied to the energy management problem for a household with a portfolio of the most prominent types of DERs. Case studies involving a real-world scenario are used to validate the superior performance of the proposed method in reducing the household’s energy costs while coping with the multi-source uncertainties through comprehensive comparisons with the state-of-the-art deep reinforcement learning (DRL) methods.
Insecticidal Activity of 25 Essential Oils on the Stored Product Pest, Sitophilus granarius
Authors: Demeter Sébastien , Lebbe Olivier , Hecq Florence , Nicolis Stamatios C. , Kenne Kemene Tierry , Martin Henri , Fauconnier Marie-Laure , Hance Thierry
Published: January 2021

The granary weevil Sitophilus granarius is a stored product pest found worldwide. Environmental damages, human health issues and the emergence of resistance are driving scientists to seeks alternatives to synthetic insecticides for its control. With low mammal toxicity and low persistence, essential oils are more and more being considered a potential alternative. In this study, we compare the toxicity of 25 essential oils, representing a large array of chemical compositions, on adult granary weevils. Bioassays indicated that Allium sativum was the most toxic essential oil, with the lowest calculated lethal concentration 90 (LC90) both after 24 h and 7 days. Gaultheria procumbens, Mentha arvensis and Eucalyptus dives oils appeared to have a good potential in terms of toxicity/cost ratio for further development of a plant-derived biocide. Low influence of exposure time was observed for most of essential oils. The methodology developed here offers the possibility to test a large array of essential oils in the same experimental bioassay and in a standardized way. It is a first step to the development of new biocide for alternative management strategies of stored product pests.
Late-Maturity Alpha-Amylase in Wheat (Triticum aestivum) and Its Impact on Fresh White Sauce Qualities
Authors: Neoh Galex K. S. , Dieters Mark J. , Tao Keyu , Fox Glen P. , Nguyen Phuong T. M. , Gilbert Robert G.
Published: January 2021

When wheat experiences a cold-temperature ‘shock’ during the late stage of grain filling, it triggers the abnormal synthesis of late-maturity α-amylase (LMA). This increases the enzyme content in affected grain, which can lead to a drastic reduction in falling number (FN). By commercial standards, a low FN is taken as an indication of inferior quality, deemed unsuitable for end-product usage. Hence, LMA-affected grains are either rejected or downgraded to feed grade at the grain receiving point. However, previous studies have found no substantial correlation between low FN-LMA and bread quality. The present study extends previous investigations to semi-solid food, evaluating the physical quality of fresh white sauce processed from LMA-affected flour. Results show that high-LMA flours had low FNs and exhibited poor pasting characteristics. However, gelation occurred in the presence of other components during fresh white sauce processing. This demonstrates that LMA-affected flours may have new applications in low-viscosity products.
Rapid Screening of Mentha spicata Essential Oil and L-Menthol in Mentha piperita Essential Oil by ATR-FTIR Spectroscopy Coupled with Multivariate Analyses
Authors: Taylan Osman , Cebi Nur , Sagdic Osman
Published: January 2021

Mentha piperita essential oil (EO) has high economic importance because of its wide usage area and health-beneficial properties. Besides health-beneficial properties, Mentha piperita EO has great importance in the flavor and food industries because of its unique sensory and quality properties. High-valued essential oils are prone to being adulterated with economic motivations. This kind of adulteration deteriorates the quality of authentic essential oil, injures the consumers, and causes negative effects on the whole supply chain from producer to the consumer. The current research used fast, economic, robust, reliable, and effective ATR-FTIR spectroscopy coupled chemometrics of hierarchical cluster analysis(HCA), principal component analysis (PCA), partial least squares regression (PLSR) and principal component regression (PCR) for monitoring of Mentha spicata EO and L-menthol adulteration in Mentha piperita EOs. Adulterant contents (Mentha spicata and L-menthol) were successfully calculated using PLSR and PCR models. Standard error of the cross-validation SECV values changed between 0.06 and 2.14. Additionally, bias and press values showed alteration between 0.06 and1.43 and 0.03 and 41.15, respectively. Authentic Mentha piperita was successfully distinguished from adulterated samples, Mentha spicata and L-menthol, by HCA and PCA analysis. The results showed that attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, coupled with chemometrics could be effectively used for monitoring various adulterants in essential oils.