bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116
Authors: Wu Yajiao , Chen Xiaoying , Bao Wenqiang , Hong Xinyu , Li Chutao , Lu Jiatong , Zhang Dongcheng , Zhu An
Published: April 2022

Humantenine, an alkaloid isolated from the medicinal herb Gelsemium elegans (Gardner & Chapm.) Benth., has been reported to induce intestinal irritation, but the underlying toxicological mechanisms remain unclear. The object of the present study was to investigate the RNA N6-methyladenosine (m6A) modification and distinct mRNA transcriptome profiles in humantenine-treated HCT116 human colon cancer cells. High-throughput MeRIP-seq and mRNA-seq were performed, and bioinformatic analysis was performed to reveal the role of abnormal RNA m6A modification and mRNA expression in humantenine-induced intestinal cell toxicity. After humantenine treatment of HCT116 cells, 1401 genes were in the overlap of differentially m6A-modified mRNA and differentially expressed mRNA. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology annotation terms for actin cytoskeleton, tight junctions, and adherens junctions were enriched. A total of 11 kinds of RNA m6A methylation regulators were differentially expressed. The m6A methylation levels of target genes were disordered in the humantenine group. In conclusion, this study suggested that the HCT116 cell injury induced by humantenine was associated with the abnormal mRNA expression of m6A regulators, as well as disordered m6A methylation levels of target genes.
New Variant in Placophilin-2 Gene Causing Arrhythmogenic Myocardiopathy
Authors: Caimi-Martinez Fiama , Antoniutti Guido , Blanco Rocio , García de la Villa Bernardo , Alvarenga Nelson , Govea-Callizo Nancy , Torres-Juan Laura , Heine-Suñer Damián , Rosell-Andreo Jordi , Luengos David Crémer , Alvarez-Rubio Jorge , Ripoll-Vera Tomás
Published: April 2022

Introduction: Arrhythmogenic cardiomyopathy (ACM) is an inherited disease characterized by progressive fibroadipose replacement of cardiomyocytes. Its diagnosis is based on imaging, electrocardiographic, histological and genetic/familial criteria. The development of the disease is based mainly on desmosomal genes. Knowledge of the phenotypic expression of each of these genes will help in both diagnosis and prognosis. The objective of this study is to describe the genotype–phenotype association of an unknown PKP2 gene variant in a family diagnosed with ACM. Methods: Clinical and genetic study of a big family carrying the p.Tyr168* variant in the PKP2 gene, in order to demonstrate pathogenicity of this variant, causing ACM. Results: Twenty-two patients (proband and relatives) were evaluated. This variant presented with high arrhythmic load at an early age, but without evidence of structural heart disease after 20 years of follow-up, with low risk in predictive scores. We demonstrate evidence of its pathogenicity. Conclusions: The p.Tyr168* variant in the PKP2 gene causes ACM with a high arrhythmic load and with an absence of structural heart disease. This fact emphasizes the value of knowing the phenotypic expression of each variant.
Validation of a Modified Version of the German Sedentary Behavior Questionnaire
Authors: Kalisch Tobias , Theil Christoph , Gosheger Georg , Schwarze Jan , Voss Katharina , Schoenhals Isabell , Moellenbeck Burkhard
Published: April 2022

Background: Physical inactivity and excessive sedentary behavior (SB) are growing public health issues that require surveillance, guidelines, and targeted interventions. In addition to a variety of sophisticated technical methods, questionnaires are still an attractive method for quick, easy, comprehensive, and cost-effective estimation of SB. The aim of this study was to validate a modified version of the widely used Sedentary Behavior Questionnaire (SBQ) compared to waist-worn accelerometers as an objective measurement. Contemporary explanations covering the use of smart devices have been added to the original instrument, and sitting while handwriting was explicated in more detail. Methods: Cross-sectional data from an adult sample (n = 64, 20–85 y, 25 m, 39 f) were used in this first validation study. Based on prior investigations of the SBQ, analyses were conducted in a gender-specific manner. Criterion validity was assessed using Spearman’s Rho coefficients. The Bland–Altman method was used to test the agreement between self-reported and accelerometer-measured SB time. Results: Using the modified SBQ (mSBQ), a significant gender difference in weekly sedentary time was found. Women estimated their sedentary time to be almost 50% higher than men (median 74.5 h vs. 51.0 h). No correlation was found between the questionnaire and accelerometer data for both subgroups (rho ≤ 0.281, p ≥ 0.174). Individual differences in daily SB estimation between both methods (in relation to accelerometry) were +3.82 h ± 4.36 h for women and +0.48 h ± 2.58 h for men (p < 0.001). Conclusion: The modifications to the SBQ did not improve the correlation between self-assessment of SB and objective determination. The reasons for the presented gender-specific overestimation of the participants’ own sedentary time, which contradicts the findings of other studies, remain unclear and need to be investigated further.
Clinical Factors Associated with COVID-19 Severity in Chronic Hospitalized Infants and Toddlers: Data from a Center in the West Part of Romania
Authors: Domnicu Alina , Mogoi Mirela , Manea Aniko , Boia Eugen Radu , Boia Marioara
Published: April 2022

Background: The risk factors for developing a severe form of COVID-19 in young children are poorly understood. Methods: A single-center retrospective study was conducted to quantify and analyze the clinical risk profile of children admitted to the Pediatric Clinic for Nutritional Recovery. Results: Overall, 51.5% (n = 17) of children were infected with SARS-CoV-2, all symptomatic, and five of them (29.4%) developed a severe form. A positive clinical pulmonary exam was only associated with the severe outcome (OR: 2.00; 95% CI, 0.33–5.66; p = 0.02). Other factors such as age under 3 months, prematurity, birth weight, malnutrition or positive history of congenital cardiac, neurodevelopmental, or genetic diseases, fever, temperature, cough, and digestive symptoms were not found to be significant risk factors. Conclusions: Clinical guidelines based on risk stratification for SARS-CoV-2 infection in children are needed in order to manage, monitor and establish priority access for some groups to high medical care.
SOFA Score Plus Impedance Ratio Predicts Mortality in Critically Ill Patients Admitted to the Emergency Department: Retrospective Observational Study
Authors: Kammar-García Ashuin , Castillo-Martínez Lilia , Mancilla-Galindo Javier , Villanueva-Juárez José Luis , Pérez-Pérez Anayeli , Rocha-González Héctor Isaac , Arrieta-Valencia Jesús , Remolina-Schlig Miguel , Hernández-Gilsoul Thierry
Published: April 2022

Background: The Sequential Organ Failure Assessment (SOFA) is a scoring system used for the evaluation of disease severity and prognosis of critically ill patients. The impedance ratio (Imp-R) is a novel mortality predictor. Aims: This study aimed to evaluate the combination of the SOFA + Imp-R in the prediction of mortality in critically ill patients admitted to the Emergency Department (ED). Methods: A retrospective cohort study was performed in adult patients with acute illness admitted to the ED of a tertiary-care referral center. Baseline SOFA score and bioelectrical impedance analysis to obtain the Imp-R were performed within the first 24 h after admission to the ED. A Cox regression analysis was performed to evaluate the mortality risk of the initial SOFA score plus the Imp-R. Harrell’s C-statistic and decision curve analyses (DCA) were performed. Results: Out of 325 patients, 240 were included for analysis. Overall mortality was 31.3%. Only 21.3% of non-surviving patients died after hospital discharge, and 78.4% died during their hospital stay. Of the latter, 40.6% died in the ED. The SOFA and Imp-R values were higher in non-survivors and were significantly associated with mortality in all models. The combination of the SOFA + Imp-R significantly predicted 30-day mortality, in-hospital mortality, and ED mortality with an area under the curve (AUC) of 0.80 (95% CI: 74–0.86), 0.79 (95% CI: 0.74–0.86) and 0.75 (95% CI: 0.66–0.84), respectively. The DCA showed that combining the SOFA + Imp-R improved the prediction of mortality through the lower risk thresholds. Conclusions: The addition of the Imp-R to the baseline SOFA score on admission to the ED improves mortality prediction in severely acutely ill patients admitted to the ED.
On the Accuracy of Particle Image Velocimetry with Citizen Videos—Five Typical Case Studies
Authors: Rozos Evangelos , Mazi Katerina , Lykoudis Spyridon
Published: April 2022

The application of image velocimetry to measure surface streamflow velocities requires meticulous preparation, including surveying and securing both the existence of floating features on the water surface, and, as in every hydrometry method, appropriate hydraulic conditions (e.g., uniform flow, turbulent velocity profile, etc.). Though these requirements can be easily satisfied when all stages involved in image velocimetry are prepared and executed by specialists, this is not guaranteed when the video footage is recorded by citizens. This kind of spontaneously obtained data are frequently the only available information of extreme flood events; therefore, and despite their non-scientific origin and standardization, these data are very important for hydrology. In this study, we evaluate image velocimetry under a variety of conditions, including conditions resembling citizen videos. Furthermore, we conclude on the manual analysis as a means of verification of the accuracy of the velocity estimations. An interesting finding from the case study with non-uniform flow conditions was that the surface velocities occurring at the middle section of the river, estimated using large-scale particle image velocimetry algorithms, exhibited a significant error, whereas the manual estimation was more accurate. This finding calls for further investigation and a more careful approach in similar conditions.
A New Approach to the Optimization of Looped Water Distribution Networks with Velocity Constraints
Authors: Manolis Antonis , Sidiropoulos Epaminondas
Published: April 2022

The optimal design of a looped water distribution system is a problem that is addressed frequently in the literature. Usually, the flow velocity in the pipes is not taken into account. Nevertheless, in real-life applications, there are velocity restrictions that must be considered for the proper function of water distribution systems. An algorithm has been presented recently for the optimal design of such systems, relying entirely on the hydraulic characteristics of the system, and not involving any parameters to be adjusted. This paper presents a new suitably designed algorithm that retained the features of the original algorithm and handled the problem of velocity restrictions without recourse to penalty functions. The new algorithm was tested and compared with others that used penalty functions to handle the velocity constraints. The results demonstrated its efficiency, reliability, and better performance.
Sexually Transmitted Diseases: Diagnosis and Control
Authors: Fasciana Teresa , Capra Giuseppina , Lipari Dario , Firenze Alberto , Giammanco Anna
Published: April 2022

Sexually transmitted diseases (STDs)—or sexually transmitted infections (STIs)—are generally acquired through sexual contact [...]
A Cross-Sectional Study of Ocular Changes in Children and Adolescents with Diabetes Mellitus in Selected Health Facilities in Ghana
Authors: Essuman Vera A. , Tagoe Naa N. , Essuman Akye , Abaidoo Benjamin , Akpalu Josephine , Sackey Harold A. , Hayfron-Benjamin Charles F. , Asare George , Amoah Albert G. B. , Ndanu Thomas A. , Ofori-Adjei Imelda D. B. , Barnes Nana A. , Appiah-Thompson Benedicta L. , Amoaku Winfried M.
Published: April 2022

Background: The main objective of the study was to determine the prevalence of diabetic retinopathy (DR), other diabetes-related ocular changes (e.g., cataracts, corneal ulceration), and non-diabetic ocular disease in Ghanaian children and adolescents. The second objective was to evaluate the relationship between these conditions and age at diagnosis, current age, diabetes mellitus (DM) duration, and participant’s sex. Methods: A cross-sectional study, undertaken by a multidisciplinary team, included a cohort of children and adolescents (4–19 years) with DM recruited from selected health facilities in Ghana, from March 2016 to September 2019, after written informed consent or assent. The cohort will be followed up for 3 years to determine the natural course of the ocular changes, reported later. Participants were examined for all microvascular and macrovascular complications, non-diabetic ocular disease, anthropometric measurements, laboratory characteristics and quality of life issues. Full ocular examination was also undertaken. Statistical Package for Social Sciences (SPSS Version 25.0) was used for the data analysis. Continuous and categorical variables were presented as mean and standard deviation (SD), median (interquartile range) and as percentages (%), respectively. T-test and Mann–Whitney U test were used in establishing associations. Results: A total of 58 participants were recruited. DR was detected in only 1 out of 58 (1.7%) participants at baseline. Cataracts were the most common ocular finding, detected in 42 (72%) at baseline. Other anterior segment changes observed included blepharitis 46 (79.3%) and tear film instability 38 (65.5%). There was a significant positive association between duration of the DM and the risk of cataract (p = 0.027). Participants’ age at diagnosis was significantly associated with the presence of prominent corneal nerves (p = 0.004). Conclusions: DR was uncommon in this cohort of young persons with DM in Ghana. Cataracts, blepharitis and refractive errors were ocular changes commonly observed. All young persons with diabetes should undergo regular eye examination in all clinics where follow-up care is provided.
Association between Diet, Physical Activity and Nutritional Status of Male Border Guard Officers
Authors: Anyżewska Anna , Łakomy Roman , Lepionka Tomasz , Maculewicz Ewelina , Szarska Ewa , Tomczak Andrzej , Bolczyk Izabela , Bertrandt Jerzy
Published: April 2022

The main factors that determine the effectiveness and reliability of duties and tasks performed by border guard officers, are very good health and maintaining a high level of psychophysical fitness that depend mainly on adequate diet and physical activity and thus, nutritional status. The aim of the study was to verify the correlations between dietary habits, physical activity level and selected nutritional status indicators. One hundred and sixty-nine male border guard officers participated in the study. A 61-item food frequency questionnaire (FFQ) was used to assess dietary habits and a long-form International Physical Activity Questionnaire (IPAQ) was used to assess physical activity. Fat mass was determined by bioelectrical impedance analysis (BIA) and bone calcification was assessed by the dual energy X-ray absorptiometry (DXA). Many correlations between dietary habits, as well as the physical activity of officers and body mass index (BMI), fat mass index (FMI) and visceral fat level (VFL) were found, while bone mineral density (BMD T-score) negatively correlated only with two food groups and 6 out of 61 products but did not correlate with physical activity. The results also confirmed many poor dietary habits and abnormalities in nutritional status. Thus, there is a need for nutritional education and further monitoring of health-related behaviors, as well as monitoring the nutritional status of border guard officers.
Human Development Index Is Associated with COVID-19 Case Fatality Rate in Brazil: An Ecological Study
Authors: Palamim Camila Vantini Capasso , Boschiero Matheus Negri , Valencise Felipe Eduardo , Marson Fernando Augusto Lima
Published: April 2022

The Human Development Index measures a region’s development and is a step for development debate beyond the traditional, economic perspective. It can also determine the success of a country’s response to the COVID-19 pandemic, mainly affecting the case fatality rate among severe cases of SARS-CoV-2 infection. We aimed to associate the Human Development Index with the case fatality rate due to COVID-19 in each Brazilian state and the Federal District, taking into account comorbidities and the need for invasive mechanical ventilation. We also evaluated the influence of the GINI index, number of intensive care unit beds, and occupied households in subnormal clusters on the case fatality rate. We performed an ecological study including two populations: COVID-19 individuals that did not require the mechanical ventilation protocol; and COVID-19 individuals under invasive mechanical ventilation. We performed a Pearson correlation test and a univariate linear regression analysis on the relationship between Human Development Index, Human Development Index—Education Level, Human Development Index—Life Expectancy, and Human Development Index—Gross National Income per capita and COVID-19 deaths. The same analyses were performed using the other markers. We grouped the patients with COVID-19 according to comorbidities and the need for invasive mechanical ventilation. Alpha = 0.05. We included 848,501 COVID-19 individuals, out of which 153,710 needed invasive mechanical ventilation and 314,164 died, and 280,533 COVID-19 individuals without comorbidity, out of which 33,312 needed invasive mechanical ventilation and 73,723 died. We observed a low negative Pearson correlation between the Human Development Index and death and a moderate negative Pearson correlation between the Human Development Index and deaths of individuals on invasive mechanical ventilation, with or without comorbidity. The univariate linear analysis showed the case fatality rate depends on at least 20–40% of the Human Development Index. In Brazil, regions with a low Human Development Index demonstrated a higher case fatality rate due to COVID-19, mainly in individuals who needed invasive mechanical ventilation, than regions with a higher Human Development Index. Although other indexes studied, such as intensive care unit beds and GINI, were also associated with the COVID-19 case fatality rate, they were not as relevant as the Human Development Index. Brazil is a vast territory comprising cultural, social, and economic diversity, which mirrors the diversity of the Human Development Index. Brazil is a model nation for the study of the Human Development Index’s influence on aspects of the COVID-19 pandemic, such as its impact on the case fatality rate.
Endometriosis and Sexual Functioning: How Much Do Cognitive and Psycho-Emotional Factors Matter?
Authors: Rossi Valentina , Galizia Roberta , Tripodi Francesca , Simonelli Chiara , Porpora Maria , Nimbi Filippo
Published: April 2022

Women with endometriosis often suffer from genito-pelvic pain. The objective of the present study was to analyze the relationship between cognitive and psycho-emotional factors and sexual functioning of women with endometriosis, comparing women with and without endometriosis. A total of 87 women with endometriosis (EG) and 100 women without endometriosis (CG) completed a socio-demographic questionnaire; the short-form of McGill Pain Questionnaire (SF-MPQ); the Female Sexual Functioning Index (FSFI); the Sexual Distress Scale (SDS); the Symptoms Checklist (SCL-90-R); the Toronto Alexithymia Scale (TAS-20); the Positive and Negative Affects Scale (PANAS); the Sexual Dysfunctional Belief Questionnaire (SDBQ); the Sexual Modes Questionnaire (SMQ); and the Questionnaire of Cognitive Schema Activation in Sexual Context (QCSASC). EG obtained worse scores than CG in SF-MPQ, Pain subscale of FSFI, and SDS. EG reached higher scores than CG on almost all scales of SCL-90-R and lower scores in the “Identifying Feelings” scale of TAS-20. Furthermore, EG reported more negative emotions toward sexuality than CG (PANAS) higher scores on the “Affection Primacy” scale of SDBQ and the “Helpless” sexual cognitive schema of QCSASC than CG. No significant differences were found in SMQ. Sexual health professionals should address psychological, emotional, and cognitive factors since they seem involved in patients’ sexual experiences.
At-Home Orthodontic Treatment for Severe Teeth Arch Malalignment and Severe Obstructive Sleep Apnea Syndrome in a Child with Cerebral Palsy
Authors: Tamura Atsuko , Yamaguchi Kohei , Yanagida Ryosuke , Miyata Rie , Tohara Haruka
Published: April 2022

Children with cerebral palsy typically have severe teeth arch malalignment, causing swallowing and respiration dysfunction. Malalignment in cerebral palsy, especially in children, worsens dysphagia and respiratory disorders; sometimes, it is also noted with obstructive sleep apnea. However, no study has reported on the improvement in obstructive sleep apnea after at-home orthodontic treatment in children with cerebral palsy. We herein present a pediatric case of cerebral palsy wherein obstructive sleep apnea improved with at-home orthodontic treatment for malalignment. We administered at-home orthodontic treatment to a 15-year-old boy with quadriplegia, due to spastic-type cerebral palsy, having no oral intake, obstructive sleep apnea, and teeth arch malalignment. After treatment, a decline in the severity of sleep apnea was observed. Perioral muscle hypertension and oral intake difficulties cause maxillary protrusion, narrowed teeth arch, and tilting of teeth in children with cerebral palsy. We expanded the oral cavity volume by orthodontic treatment to relieve muscle hypertension and correct the tongue position, thereby remarkably improving obstructive sleep apnea. Our findings suggest that at-home orthodontic treatment for malalignment effectively improves perioral muscle hypertension, glossoptosis, and obstructive sleep apnea.
The Prognostic Role of miR-31 in Head and Neck Squamous Cell Carcinoma: Systematic Review and Meta-Analysis with Trial Sequential Analysis
Authors: Dioguardi Mario , Spirito Francesca , Sovereto Diego , Alovisi Mario , Aiuto Riccardo , Garcovich Daniele , Crincoli Vito , Laino Luigi , Cazzolla Angela Pia , Caloro Giorgia Apollonia , Di Cosola Michele , Ballini Andrea , Lo Muzio Lorenzo , Troiano Giuseppe
Published: April 2022

Background: Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide with high recurrence, metastasis, and poor treatment outcome. Prognostic survival biomarkers can be a valid tool for assessing a patient’s life expectancy and directing therapy toward specific targets. Recent studies have reported microRNA (miR) might play a critical role in regulating different types of cancer. The main miR used as a diagnostic and prognostic biomarker and reported in the scientific literature for HNSCC is miR-21. Other miRs have been investigated to a lesser extent (miR-99a, miR-99b, miR-100, miR-143, miR-155, miR-7, miR-424, miR-183), but among these, the one that has attracted major interest is the miR-31. Methods: The systematic review was conducted following the PRISMA guidelines using electronic databases, such as PubMed, Scopus, and the Cochrane Central Register of Controlled Trials, with the use of combinations of keywords, such as miR-31 AND HNSCC, microRNA AND HNSCC, and miR-31. The meta-analysis was performed using the RevMan 5.41 software (Cochrane Collaboration, Copenhagen, Denmark). Results: This search produced 721 records, which, after the elimination of duplicates and the application of the inclusion and exclusion criteria, led to 4 articles. The meta-analysis was conducted by applying fixed-effects models, given the low rate of heterogeneity (I2 = 40%). The results of the meta-analysis report an aggregate hazard ratio (HR) for the overall survival (OS), between the highest and lowest miR-31 expression, of 1.59, with the relative intervals of confidence (1.22 2.07). Heterogeneity was evaluated through Chi2 = 5.04 df = 3 (p = 0.17) and the Higgins index I2 = 40; testing for the overall effect was Z = 3.44 (p = 0.00006). The forest plot shows us a worsening HR value of OS, in relation to the elevated expression of miR-31. Conclusions: In conclusion, the data resulting from the current meta-analysis suggest that miR-31 is associated with the prognosis of patients with HNSCC and that elevated miR-31 expression could predict a poor prognosis in patients with this type of neoplasm.
Environmental Risk and Credit Ratings, and the Moderating Effect of Market Competition
Authors: Kim Yujin , Kim Su-In
Published: April 2022

This study examines the relationship between environmental risk and corporate bond credit ratings, and the moderating effect of market competition. We focus on Korean firms that are facing increasing risk of environmental crisis after the COVID-19 pandemic. Recently, the Korean government has been controlling businesses while promoting policies to transform the economy into a low-energy, low-carbon economy. We find that a firm’s greenhouse gas emission and energy consumption, which are direct indicators of environmental risk, are negatively associated with bond credit ratings. We also report that the negative effect of environmental risk on credit ratings is stronger in firms with low market competition. This study contributes to prior research by improving the understanding of the effect of environmental risk on credit ratings. In particular, it is significant to examine the effect of environmental risk, measured as direct environmental performance not affected by green washing, on credit rating. Therefore, we shed light on environment-oriented management beyond the determinants of credit ratings, which have been discussed in previous studies. We also suggest that policymakers need to manage market competition in terms of environmental justice, given that market competition has a significant moderating effect on the relationship between environmental risk and credit ratings.
The Effects of the Opioid Crisis on Agricultural Industries
Authors: Radunovich Heidi Liss , Younker Terasa , Rung Jillian M. , Berry Meredith S.
Published: April 2022

Opioid use remains a significant public health crisis. However, few quantitative or qualitative data exist on the prevalence of opioid use and associated mental health conditions in agricultural industries and how it affects the industries themselves. Data on opioid use and associated consequences were collected among agricultural business owners and workers using both quantitative (n = 129) and qualitative assessment (n = 7). The prevalence of opioid use, pain, stress, and depressive symptoms as well as associated hazards were characterized among individuals who work in horticulture (nursery and landscape) and those who work in food production (livestock and crops). Qualitative interviews were also conducted to better understand individual experiences with opioid use. Opioid use was significantly higher among horticultural industries compared to food production. Pain and depressive scores were higher among those who had used opioids although stress did not differ. Importantly, substantial percentages of participants who reported opioid use also reported consequences associated with their use, including missing work, being injured at work while using, and having difficulty in completing daily tasks. These results provide initial evidence that opioid use is substantially affecting agricultural industries in terms of mental health, personal health, labor availability, and productivity.
Effect of an Electronic Alert System on Hepatitis B Virus Reactivation in Patients Receiving Immunosuppressive Drug Therapy
Authors: Asai Akira , Hirai Saho , Yokohama Keisuke , Nishikawa Tomohiro , Nishikawa Hiroki , Higuchi Kazuhide
Published: April 2022

Hepatitis B virus (HBV) reactivation (HBVr) can occur in patients receiving immunosuppressive drug therapies, causing significant morbidity and mortality. Although the guidelines for HBVr have been proposed by several academic societies, some providers do not follow them, resulting in HBVr and death. As HBV-DNA levels increase before liver enzyme levels do, we previously constructed an electronic alert system that recommends the measurement of HBV-DNA. Here, we investigated whether this alert system improves the HBV-DNA measurement rate and elicits responses according to guidelines. A total of 5329 patients were divided into two groups, before and after the introduction of the alert system, and the HBV-DNA measurement rates in both groups were compared. Because of the introduction of the alert system, the HBV-DNA measurement rate among HBsAg-negative patients with anti-HBs and/or anti-HBc before immunosuppressive drug therapy improved significantly. The HBV-DNA monitoring rate within 3 months also improved significantly (p = 0.0034) in HBV-remission phase patients. HBVr was detected immediately, and the affected patients were treated with nucleotide analogs before severe hepatitis onset. The introduction of the alert system for HBVr improved the HBV-DNA measurement rates in patients receiving immunosuppressive drug therapy, leading to the rapid treatment of patients with HBVr.
Impact of the COVID-19 Pandemic on the Clinical Profile of Candidemia and the Incidence of Fungemia Due to Fluconazole-Resistant Candida parapsilosis
Authors: Ramos-Martínez Antonio , Pintos-Pascual Ilduara , Guinea Jesús , Gutiérrez-Villanueva Andrea , Gutiérrez-Abreu Edith , Díaz-García Judith , Asensio Ángel , Iranzo Reyes , Sánchez-Romero Isabel , Muñoz-Algarra María , Moreno-Torres Víctor , Calderón-Parra Jorge , Múñez Elena , Fernández-Cruz Ana
Published: April 2022

Severely ill COVID-19 patients are at high risk of nosocomial infections. The aim of the study was to describe the characteristics of candidemia during the pre-pandemic period (January 2019–February 2020) compared to the pandemic period (March 2020–September 2021). Antifungal susceptibilities were assessed using the EUCAST E.Def 7.3.2 broth dilution method. Fluconazole-resistant C. parapsilosis isolates (FRCP) were studied for sequencing of the ERG11 gene. The incidence of candidemia and C. parapsilosis bloodstream infection increased significantly in the pandemic period (p = 0.021). ICU admission, mechanical ventilation, parenteral nutrition and corticosteroids administration were more frequent in patients with candidemia who had been admitted due to COVID-19. Fifteen cases of FRCP fungemia were detected. The first case was recorded 10 months before the pandemic in a patient transferred from another hospital. The incidence of FRCP in patients admitted for COVID-19 was 1.34 and 0.16 in all other patients (p < 0.001). ICU admission, previous Candida spp. colonization, arterial catheter use, parenteral nutrition and renal function replacement therapy were more frequent in patients with candidemia due to FRCP. All FRCP isolates showed the Y132F mutation. In conclusion, the incidence of candidemia experienced an increase during the COVID-19 pandemic and FRCP fungemia was more frequent in patients admitted due to COVID-19.
Correlation Assessment of the Performance of Associative Classifiers on Credit Datasets Based on Data Complexity Measures
Authors: Camacho-Urriolagoitia Francisco J. , Villuendas-Rey Yenny , López-Yáñez Itzamá , Camacho-Nieto Oscar , Yáñez-Márquez Cornelio
Published: April 2022

One of the four basic machine learning tasks is pattern classification. The selection of the proper learning algorithm for a given problem is a challenging task, formally known as the algorithm selection problem (ASP). In particular, we are interested in the behavior of the associative classifiers derived from Alpha-Beta models applied to the financial field. In this paper, the behavior of four associative classifiers was studied: the One-Hot version of the Hybrid Associative Classifier with Translation (CHAT-OHM), the Extended Gamma (EG), the Naïve Associative Classifier (NAC), and the Assisted Classification for Imbalanced Datasets (ACID). To establish the performance, we used the area under the curve (AUC), F-score, and geometric mean measures. The four classifiers were applied over 11 datasets from the financial area. Then, the performance of each one was analyzed, considering their correlation with the measures of data complexity, corresponding to six categories based on specific aspects of the datasets: feature, linearity, neighborhood, network, dimensionality, and class imbalance. The correlations that arise between the measures of complexity of the datasets and the measures of performance of the associative classifiers are established; these results are expressed with Spearman’s Rho coefficient. The experimental results correctly indicated correlations between data complexity measures and the performance of the associative classifiers.
1H-Pyrazolo[3,4-b]quinolines: Synthesis and Properties over 100 Years of Research
Authors: Danel Andrzej , Gondek Ewa , Kucharek Mateusz , Szlachcic Paweł , Gut Arkadiusz
Published: April 2022

This paper summarises a little over 100 years of research on the synthesis and the photophysical and biological properties of 1H-pyrazolo[3,4-b]quinolines that was published in the years 1911–2021. The main methods of synthesis are described, which include Friedländer condensation, synthesis from anthranilic acid derivatives, multicomponent synthesis and others. The use of this class of compounds as potential fluorescent sensors and biologically active compounds is shown. This review intends to summarize the abovementioned aspects of 1H-pyrazolo[3,4-b]quinoline chemistry. Some of the results that are presented in this publication come from the laboratories of the authors of this review.
Cryptosporidium: Still Open Scenarios
Authors: Pane Stefania , Putignani Lorenza
Published: April 2022

Cryptosporidiosis is increasingly identified as a leading cause of childhood diarrhea and malnutrition in both low-income and high-income countries. The strong impact on public health in epidemic scenarios makes it increasingly essential to identify the sources of infection and understand the transmission routes in order to apply the right prevention or treatment protocols. The objective of this literature review was to present an overview of the current state of human cryptosporidiosis, reviewing risk factors, discussing advances in the drug treatment and epidemiology, and emphasizing the need to identify a government system for reporting diagnosed cases, hitherto undervalued.
Improved Characteristics of RANKL Immuno-PET Imaging Using Radiolabeled Antibody Fab Fragments
Authors: Dewulf Jonatan , Hrynchak Ivanna , Geudens Sarah , Pintelon Isabel , Vangestel Christel , Sereno José , van Dam Peter A. , Abrunhosa Antero J. , Elvas Filipe , Van den Wyngaert Tim
Published: April 2022

Purpose: RANKL expression in the tumor microenvironment has been identified as a biomarker of immune suppression, negating the effect of some cancer immunotherapies. Previously we had developed a radiotracer based on the FDA-approved RANKL-specific antibody denosumab, [89Zr]Zr-DFO-denosumab, enabling successful immuno-PET imaging. Radiolabeled denosumab, however, showed long blood circulation and delayed tumor uptake, potentially limiting its applications. Here we aimed to develop a smaller radiolabeled denosumab fragment, [64Cu]Cu-NOTA-denos-Fab, that would ideally show faster tumor accumulation and better diffusion into the tumor for the visualization of RANKL. Experimental design: Fab fragments were prepared from denosumab using papain and conjugated to a NOTA chelator for radiolabeling with 64Cu. The bioconjugates were characterized in vitro using SDS-PAGE analysis, and the binding affinity was assessed using a radiotracer cell binding assay. Small animal PET imaging evaluated tumor targeting and biodistribution in transduced RANKL-ME-180 xenografts. Results: The radiolabeling yield of [64Cu]Cu-NOTA-denos-Fab was 58 ± 9.2%, with a specific activity of 0.79 ± 0.11 MBq/µg (n = 3). A radiotracer binding assay proved specific targeting of RANKL in vitro. PET imaging showed fast blood clearance and high tumor accumulation as early as 1 h p.i. (2.14 ± 0.21% ID/mL), which peaked at 5 h p.i. (2.72 ± 0.61% ID/mL). In contrast, [64Cu]Cu-NOTA-denosumab reached its highest tumor uptake at 24 h p.i. (6.88 ± 1.12% ID/mL). [64Cu]Cu-NOTA-denos-Fab specifically targeted human RANKL in transduced ME-180 xenografts compared with the blocking group and negative ME-180 xenograft model. Histological analysis confirmed RANKL expression in RANKL-ME-180 xenografts. Conclusions: Here, we report on a novel RANKL PET imaging agent, [64Cu]Cu-NOTA-denos-Fab, that allows for fast tumor imaging with improved imaging contrast when compared with its antibody counterpart, showing promise as a potential PET RANKL imaging tool for future clinical applications.
Use of Gamma Radiation for the Genetic Improvement of Underutilized Plant Varieties
Authors: Riviello-Flores María de la Luz , Cadena-Iñiguez Jorge , Ruiz-Posadas Lucero del Mar , Arévalo-Galarza Ma. de Lourdes , Castillo-Juárez Israel , Soto Hernández Marcos , Castillo-Martínez Carlos Roman
Published: April 2022

Agricultural biodiversity includes many species that have biological variants (natives, ecotypes, races, morphotypes). Their use is restricted to local areas because they do not fulfill the commercial requirements; however, it is well documented that these species are a source of metabolites, proteins, enzymes, and genes. Rescuing and harnessing them through traditional genetic breeding is time-consuming and expensive. Inducing mutagenesis may be a short-time option for its genetic improvement. A review of outstanding research was carried out, in order to become familiar with gene breeding using gamma radiation and its relevance to obtain outstanding agronomic characteristics for underutilized species. An approach was made to the global panorama of the application of gamma radiation in different conventional crop species and in vitro cultivated species, in order to obtain secondary metabolites, as well as molecular tools used for mutation screening. The varied effects of gamma radiation are essentially the result of the individual responses and phenotypic plasticity of each organism. However, even implicit chance can be reduced with specific genetic breeding, environmental adaptation, or conservation objectives.
Photosynthetic Parameters and Oxidative Stress during Acclimation of Crepe-Myrtle (Lagerstroemia speciosa (L.) Pers.) in a meta-Topolin-Based Micropropagation System and Genetic Fidelity of Regenerated Plants
Authors: Ahmad Naseem , Fatima Nigar , Faisal Mohammad , Alatar Abdulrahman A. , Pathirana Ranjith
Published: April 2022

An improved and stable micropropagation system using the cytokinin, meta-Topolin (N6 (3-hydroxybenzylamino purine—mT), with nodal explants in Lagerstroemia speciosa L. was established. Among the different doses of mT, the maximum number of shoots with the highest shoot length was obtained using Murashige and Skoog’s (MS) medium supplemented with 5.0 µM mT. The results were consistent throughout the proliferation period, when recorded at week 4, 8, and 12 of being cultured, with an average of 16.4 shoots per nodal explant, and having a mean length of 4.10 cm at week 8. Shoot proliferation rates could be further improved by a combination of 5.0 µM mT with 0.5 µM α-naphthalene acetic acid in MS medium; nodal explants produced an average of 24.3 shoots with a mean length of 5.74 cm after 8 weeks of being cultured. Among the five different concentrations of three auxins tested for the rooting of microshoots in MS medium, a 1.0 µM indole-3-butyric acid treatment was the best, with an average of 10.3 roots per microshoot at an average length of 3.56 cm in 93% of microshoots within 4 weeks of being transferred to this medium. A significant reduction of both chlorophyll a and b in leaves during the first week of acclimation corresponded with a high accumulation of malondialdehyde (MDH), indicating that lipid peroxidation affected chlorophyll pigments. From the second week of acclimation, photosynthetic pigment content significantly increased and MDH content decreased. The net photosynthetic rate and leaf carotenoid content showed almost linear increases throughout the acclimation period. Activity of antioxidant enzymes, namely, superoxide dismutase, catalase, and peroxidases, consistently increased throughout the acclimation period, corresponding with the accumulation of photosynthetic pigments, thus demonstrating the role of the improved antioxidant enzymatic defense system during acclimation. A comparison of parent plant DNA with that of the greenhouse acclimated plants using random amplified polymorphic DNA and inter-simple sequence repeat markers showed a monomorphic pattern indicating genetic stability and the suitability of the method for micropropagation of L. speciosa.
Silver Birch (Betula pendula Roth.) on Dry Mineral Rather than on Deep Peat Soils Is More Dependent on Frozen Conditions in Terms of Wind Damage in the Eastern Baltic Region
Authors: Krišāns Oskars , Matisons Roberts , Vuguls Jānis , Rust Steffen , Elferts Didzis , Seipulis Andris , Saleniece Renāte , Jansons Āris
Published: April 2022

In Northern Europe, the ongoing winter warming along with increasing precipitation shortens the periods for which soil is frozen, which aggravates the susceptibility of forest stands to wind damage under an increasing frequency of severe wind events via the reduction in soil–root anchorage. Such processes are recognized to be explicit in moist and loose soils, such as deep peat, while stands on dry mineral soils are considered more stable. In the hemiboreal forest zone in the Eastern Baltics, silver birch (Betula pendula Roth.) is an economically important species widespread on mineral and peat soils. Although birch is considered to be less prone to wind loading during dormant periods, wind damage arises under moist and non-frozen soil conditions. Static tree-pulling tests were applied to compare the mechanical stability of silver birch on frozen and non-frozen freely draining mineral and drained deep peat soils. Basal bending moment, stem strength, and soil–root plate volume were used as stability proxies. Under frozen soil conditions, the mechanical stability of silver birch was substantially improved on both soils due to boosted soil–root anchorage and a concomitant increase in stem strength. However, a relative improvement in soil–root anchorage by frozen conditions was estimated on mineral soil, which might be attributed to root distribution. The soil–root plates on the mineral soil were narrower, providing lower leverage, and thus freezing conditions had a higher effect on stability. Accordingly, silver birch on peat soil had an overall higher estimated loading resistance, which suggested its suitability for forest regeneration on loose and moist soils within the Eastern Baltic region. Nevertheless, adaptive forest management supporting individual tree stability is still encouraged.
Diagnostics of Air Purification Plasma Device by Spatially Resolved Emission Spectroscopy
Authors: Zhao Wanxia , Alwahabi Zeyad T.
Published: April 2022

A non-thermal plasma, air purification device (PlasmaShield®, MD250, Keswick, SA, Australia), was investigated using spatially resolved optical emission spectroscopy. The emission spectra were measured with two spatial dimensions to analyze and identify the transition lines of excited NO–γ (A2Σ–X2Π), N2 (C3Π–B3Π), and N2+ (B2Σ–X2Σ) systems. The N2 emission band at 337 and 316 nm were used to determine the spatially resolved vibrational temperature of N2 molecules, TvibN2. It was found that the average N2 vibrational temperatures in the x and y directions are almost the same. Two key operating parameters, supplied power and air flow, influence the N2 vibrational temperature. The results demonstrate that applying higher supplied power increases the vibrational temperature, while changes in air flow velocity do not affect the vibrational temperature values. The phenomenological plasma temperature (PPT) was also estimated from the N2 vibrational temperature. It was observed that PlasmaShield® generates excited N2 and NO only within a narrow region around the discharge electrode tip (with peak intensity below 100 µm from the tip). The study also shows no presence of excited OH*, O*, and other radicals.
Effect of Conical Spiral Flow Channel and Impeller Parameters on Flow Field and Hemolysis Performance of an Axial Magnetic Blood Pump
Authors: Yang Wei-Bo , Zhou Jian , Xiao Wei-Hu , Peng Si-Jie , Hu Ye-Fa , Li Ming , Wu Hua-Chun
Published: April 2022

For a blood pump, the blood flow channel and impeller parameters directly affect the performance of the pump and the resulting blood circulation. The flow channel in particular has a great impact on the hydraulic performance of the pump (e.g., flow and pressure), which directly determines the overall performance of the blood pump. Traditional bearing-supported blood pumps can cause mechanical damage to blood cells, leading to hemolysis and thrombosis. In this study, therefore, we designed a conical spiral axial blood pump with magnetic levitation. The blood pump was supported by electrodynamic bearings in the radial direction and electromagnetic bearings in the axial direction. The impeller and the front and rear hubs were integrated to minimize blood stagnation and reduce the formation of thrombosis. The hub had a conical spiral flow channel design, which not only reduced the size of the impeller but also increased blood flow and pressure while meeting the design requirements. Computational fluid dynamics (CFD) analysis was used to analyze the flow field of the axial blood pump, a power function model was used to establish a hemolysis prediction model, and the particle tracking method was used to obtain the flow trajectories of individual blood cells, thereby predicting hemolysis-related performance of the blood pump. The simulation results showed that the main high shear stress area in the blood pump was located in the impeller inlet and the clearance between the top of the impeller and the inner chamber of the blood pump. When the hub taper angle of the blood pump was 0.72° and the clearance was 0.3 mm, the average hemolysis prediction value was 0.00216. This prediction value was smaller than that of traditional axial blood pumps. These findings can provide an important reference for the structural design of axial blood pumps and for reducing the hemolysis prediction value.
Managerial Issues Regarding the Role of Natural Gas in the Transition of Energy and the Impact of Natural Gas Consumption on the GDP of Selected Countries
Authors: Thalassinos Eleftherios , Kadłubek Marta , Thong Le Minh , Hiep Tran Van , Ugurlu Erginbay
Published: April 2022

Natural gas is considered an important bridge in the transition of energy in the world. However, the development and management of unconventional gas now face many challenges. This paper aims to provide an overview of what would be required to formulate and implement the trend of energy transition and natural gas use in the world. Selected managerial issues regarding the role of natural gas are presented, including chosen statistical data on natural gas consumption, forecasts for the demand for natural gas, and the potential of unconventional gas. The empirical part of the study examines the impact of natural gas consumption on the GDP of 14 G20 countries during the period of 1994 to 2018. The pooled mean group model (PMG) is employed in this study. Based on the cointegration test results, it was found that natural gas consumption and population have a long-run relationship with CO2 emissions. Consistent with other studies, we found a positive relationship between CO2 emissions and natural gas consumption, GDP, and population.