bluuebunny/crossref_metadata_2025 huggingface dataset:
A New Cembrane, from Soft Coral Genus Sarcophyton in Borneo
Authors: Kamada Takashi , Zanil Intan Irna , Phan Chin-Soon , Vairappan Charles Santhanaraju
Published: March 2019

One new cembrane diterpene, (1 S, 2 E, 4 R, 6 E, 8 S, 11 S, 12 S)-11,12-epoxy-8-hydroperoxy-4-hydroxy-2,6-cembradiene (1) was isolated from a population of Bornean soft coral genus Sarcophyton collected from the coastal waters Bohey Dulang, Sabah, Malaysia. Its structure was elucidated based on extensive spectroscopic analysis including NMR and HRESIMS spectra. Cytotoxic, anti-inflammatory and antibacterial activities were tested.
Methylobamine, a UVA-Absorbing Compound from the Plant-Associated Bacteria Methylobacterium sp
Authors: Kamo Tsunashi , Hiradate Syuntaro , Suzuki Ken , Fujita Ichiro , Yamaki Shinji , Yoneda Tadashi , Koitabashi Motoo , Yoshida Shigenobu
Published: March 2019

To survive in harsh environments, phyllosphere microorganisms have developed numerous adaptive strategies against stressors. One such strategy is the synthesis of compounds that absorb ultraviolet A radiation (UVA). The chemical structure of a UVA-absorbing compound from Methylobacterium sp. strain W-213 was elucidated by spectroscopic analyses. 1H, 13C, and two-dimensional nuclear magnetic resonance spectra indicated the presence of N -methyl-α-galactosamine. The entire structure was revealed by interpretation of INADEQUATE and high-resolution liquid chromatography/electrospray ionization mass spectra. The absolute configuration was established by X-ray analysis and the compound was identified as 2-{(4a R, 6 R, 7 R, 8 R, 8a R)-7,8-dihydroxy-6-hydroxymethyl-1,2-dimethyl-4a H, 6 H, 7 H, 8 H, 8a H -pyrano[2,3- b][1,4]oxazin-3-yl}-2-iminoacetic acid and named ‘methylobamine'. Methylobamine shows promise for pharmaceutical and cosmetic applications due to its high polarity, UVA absorption properties, and stability under UVA exposure.
Chemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)
Authors: Pereira Kassia M. F. , Grecco Simone S. , Figueiredo Carlos R. , Hosomi Jorge K. , Nakamura Mari U. , Lago João Henrique G.
Published: March 2019

Kalanchoe pinnata (Crassulaceae) is a medicinal plant distributed in several areas of the World. Their leaves have been used, in folk medicine, for anxiety and sleep disorders among other proposes. Chemically, this plant is composed by organic acids, flavonoids, bufadienolides, triterpenoids and some ubiquitous compounds. In this study, the fresh leaves of K. pinnata were extracted in an accelerated solvent extraction (ASE) system using H2O (cold and hot) as well as with EtOH:H2O 1:1. The obtained crude extracts were analyzed by HPLC-UV-MS. As a result, were identified organic acids and flavonoids, these being quercetin-3- O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside, kaempferol-3- O-α-L-arabinopiranosyl-(1→2)-α-L-rhamnopyranoside, quercitrin, and trans- p-coumaryl glutaric acid the main compounds in the studied extracts. Obtained results revealed the predominance of flavonoids in extracts prepared using hot H2O (70 26 α 2.19%) or EtOH:H2O 1:1 (74.65 α 5.39%) while a reduction in the amount of these compounds was observed in the extracts prepared using cold H2O (54.41 α 2.93%). Additionally, crude extracts from leaves of K. pinnata extracted with hot H2O and EtOH:H2O 1:1 showed in vitro cytotoxicity on five tested cancer cell lines: murine melanoma (B16F10), human melanoma (A2058), human cervical carcinoma (HeLa), human leukemia (HL-60), and human glioblastoma (U87-MG), with IC50 values in the range of 10.6 α 2.5-91.3 α 7.2 μg/mL. On the other hand, the extract prepared using cold H2O displayed reduced potential. These crude extracts were also tested against human foreskin fibroblasts (non-tumorigenic cells) showing IC50 values ranging from 79.5 to 90.2 μg/mL. In conclusion, our results showed the impact that different extraction methods have on the chemical composition and pharmacological activity, which are notable for the anticancer activity associated to flavonoids.
Cinobufacini from the Skin of Bufo bufo gargarizans Induces Apoptosis, Possibly via Activation of the Wnt/β-Catenin Pathway, in Human Osteosarcoma Cells
Authors: Ma Xiu-cai , Ding Hui-qiang , Shi Jian-dang , Hei Long , Niu Ning-kui , Suo Zhi-gang , Shang Yan-bing , Lin Song , Pu Fei-fei , Shao Zeng-wu
Published: March 2019

Cinobufacini (huachansu) is a traditional Chinese medicine extracted from the skin of Bufo bufo gargarizans, which is used in clinical cancer therapy. The purpose of this study was to investigate the signaling pathways regulating cinobufacini-induced apoptosis in the osteosarcoma cell line, U2OS. We used 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate the effects of cinobufacini on cell proliferation in U2OS cells. Changes in cell morphology and apoptosis were detected by TUNEL staining. The expression of apoptosis-related and Wnt/β-catenin pathway proteins was detected by immunofluorescence, RT-PCR, and western blot analysis. Our data indicated that cinobufacini significantly inhibited cell proliferation in a dose- and time-dependent manner. Marked changes in cell morphology and apoptosis rate were clearly observed after cinobufacini treatment. The Wnt/β-catenin pathway was activated, and β-catenin expression was positive in cells after treatment. Further, protein expression of bax was increased, whereas bcl-2 was decreased, resulting in an increased bax/bcl-2 ratio. Moreover, after cinobufacini treatment, the expression of Wnt/β-catenin pathway-related proteins was similar to controls. Taken together, our study indicates that cinobufacini can induce apoptosis in U2OS cells, likely through activating the Wnt/β-catenin pathway.
Cytotoxic Effect of Aeruginosin-865, Resveratrol and Capsaicin on Mouse Fibroblasts and Cells Derived from Fallow Deer
Authors: Veselá Ivana , Kolísková Petra Celá , Kuchařová Vendula , Tomenendálová Jaroslava , Kováčová Veronika , Pikula Jiří , Repková Barbora , Rapekta Polina , Hrouzek Pavel , Cheel José , Doubek Jaroslav
Published: March 2019

Natural substances offer interesting bioactivity patterns including antiproliferative, antioxidant or cytotoxic effects. However, the safety profile of many of them has not been extensively determined. In this study, the cytotoxic effect of Aeruginosin-865, resveratrol and capsaicin at different concentrations was tested on normal mouse cells (NIH/3T3) and tumour fibroblasts (WEHI-13VAR) as well as on liver- and kidney-derived cells from fallow deer. A lactate dehydrogenase cytotoxicity assay kit was used to measure cell death in response to treatment with the test substances. It was found that NIH/3T3 cells tolerated Aeruginosin-865 (10-200 μM) and resveratrol (5-100 μM) treatment without any cytotoxic effect, while capsaicin exerted a cytotoxic effect only at the highest tested concentration (200 μ M). Mouse fibrosarcoma cells were more sensitive to the cytotoxic effect of all three compounds where Aeruginosin-865 (100-200 μM) and resveratrol (50–100 μM) showed high-dose cytotoxicity and capsaicin showed low- and high-dose cytotoxicity (25 μM and 200 μ M). The three tested compounds at the highest concentrations were found to be cytotoxic to both liver- and kidney-derived cells from fallow deer. Overall, the results indicate that the cytotoxic effects of the three tested natural substances on cells derived from fallow deer and mouse tumour fibroblasts differ significantly from those exerted on normal fibroblasts. The results demonstrate the potential of these natural compounds as therapeutic agents and pave the way for future in vivo toxicological investigations.
Chemical Composition and Variability of Leaf and Berry oils from Corsican Juniperus macrocarpa
Authors: Ottavioli Joséphine , Bighelli Ange , Casanova Joseph , Tomi Félix
Published: March 2019

The chemical composition of five leaf oil samples and eighteen berry oil samples from Corsican Juniperus macrocarpa have been investigated by GC(RI), GC-MS and 13C NMR. The composition of berry oils was dominated by monoterpene hydrocarbons with α-pinene (56.4-78.9%) as main component followed by myrcene (2.2-11.9%). Germacrene D (4.5-103%) was the major sesquiterpene. The contents of the main components of leaf oils varied drastically from sample to sample: α-pinene (28.7-76.4%), δ3-carene (up to 17.3%), β-phellandrene (up to 12.3%), manoyl oxide (up to 8.1%). The occurrence of the unusual ( Z)-pentadec-6-en-2-one (0.1-1.2%) should be pointed out. Statistical analysis (Principal Component Analysis and k- means partition) suggested a unique group with atypical samples.
Chemical Composition, Antioxidant, Antimicrobial, and α-Glucosidase Activities of Essential Oils of Hornstedtia scyphifera (Zingiberaceae)
Authors: Hashim Siti Ernieyanti , Sirat Hasnah Mohd
Published: March 2019

The essential oil compositions of the rhizomes, flowers, leaves, and pseudostems of Hornstedtia scyphifera (J. Koenig) Steud were studied for the first time. A total of forty three constituents were identified from the rhizome, flower, leaf, and pseudostem oils, representing 90.9%, 82.4%, 79.7%, and 82.6%, respectively. α-Selinene was found dominant in the flower (10.8%) and pseudostem (12.4%) oils, while the rhizome and leaf oils contained germacrene D (15.5%) and camphor (12.6%) as the main constituents, respectively. The rhizome, flower, and leaf oils showed moderate activity against Gram positive bacteria with MIC values of 225 to 450 μg/mL and inhibition zones of 8.4 to 10.0 mm. The leaf oil showed good β-carotene bleaching inhibition activity (64.8%) and a low IC50 value for DPPH radical scavenging activity (650.4 μg/mL). The flower oil showed potential α-glucosidase inhibitory activity with an IC50 value of 21.44 μg/mL.
Anti-inflammatory Activity of Melampyrum barbatum and Isolation of Iridoid and Flavonoid Compounds
Authors: Háznagy-Radnai Erzsébet , Fási Laura , Wéber Edit , Pinke Gyula , Király Gergely , Sztojkov-Ivanov Anita , Gáspár Róbert , Hohmann Judit
Published: March 2019

Melampyrum barbatum Waldst. & Kit. ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M. barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 %, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M. barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.
Allelopathy for Parasitic Plant Management
Authors: Cimmino Alessio , Masi Marco , Rubiales Diego , Evidente Antonio , Fernández-Aparicio Monica
Published: March 2019

A number of parasitic plants have adapted to agricultural environments becoming weedy and posing a serious threat to important crops. Available control measures rely heavily on use of synthetic herbicides. The side effects on environmental pollution and food health of chemical control prompted studies to find alternative strategies based on the use of natural products. This article reviews plant and fungal metabolites with potential for the development of specific and efficient methods for the control of parasitic plants.
Approach to Determination a Structure – Antioxidant Activity Relationship of Selected Common Terpenoids Evaluated by ABTS•+ Radical Cation Assay
Authors: Wojtunik-Kulesza Karolina A. , Cieśla Łukasz M. , Waksmundzka-Hajnos Monika
Published: March 2019

Data available in the literature indicated various biological activities of terpenes. One of the most frequently investigated activities is the ability to scavenge free radicals. To date, studies based on the DPPH• method indicated conjugated double bonds as moieties responsible for antioxidant activity of selected terpenes. In order to verify this theory, studies based on 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) were performed. The spectrophotometric investigations explicitly revealed relatively high antioxidant activity of terpenes possessing conjugated double bonds in comparison with substances without this structural element. It has been shown that the chain-breaking antioxidant activity of terpenes strictly depends on π bonds. TEAC values and percentage scavenging of the free radicals revealed that the most promising antioxidants are α-terpinene, ocimene, pulegone and farnesene. In order to explain the reaction between terpenes and ABTS•+ free radical cation, a possible antioxidant mechanism has been proposed.
Expression of Carotenoid Biosynthetic Genes and Carotenoid Biosynthesis during Seedling Development of Momordica charantia
Authors: Cuong Do Manh , Kim Jae Kwang , Jeon Jin , Kim Tae Jin , Park Jong Seok , Park Sang Un
Published: March 2019

Carotenoids belong to a large group of secondary metabolites, and have pivotal roles in plants, including photosynthesis and phytohormone synthesis, pigmentation, and membrane stabilization. Additionally, carotenoids are potent antioxidants, and their health benefits are becoming increasingly prominent. In recent years, carotenoids have been studied in many plants. Furthermore, gene expression, as well as carotenoid accumulation in different parts of the bitter melon, has been investigated; however, it has not been studied in bitter melon seedlings. In this study, carotenoid accumulation and transcript levels of McGGPPS1, McGGPPS2, McPSY, McPDS, McZDS, McLCYB, McLCYE1, McLCYE2, McCXHB, and McZEP, involved in carotenoid biosynthesis, were analyzed during seedling development using HPLC and qRT-PCR. The major carotenoids that accumulated in the bitter melon seedlings were lutein and E-β-carotene. The expression of most carotenoid biosynthetic genes increased during seedling development, consistent with the accumulation of violaxanthin, lutein, zeaxanthin, β-cryptoxanthin, 13Z-β-carotene, E-β-carotene, and 9Z-β-carotene in bitter melon seedlings. The results of this study provide a firm basis for comprehending the link between gene expression and carotenoid concentration in bitter melon seedlings.
Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics
Authors: Milenković Marina , Stošović Jelena , Slavkovska Violeta
Published: March 2019

The subject of the study was the investigation of the chemical composition and antimicrobial activity of the essential oils (EOs) isolated from Calamintha sylvatica, C. vardarensis, C. nepeta and C. glandulosa, as well as their antibacterial activity in combination with antibiotics. The quantitative and qualitative analysis of EOs was performed using the GC/FID and GC/MS methods. The antimicrobial activity of EOs against six standard bacterial strains and one strain of yeast was tested using the broth microdilution method, while the antimicrobial activity of a combination of essential oils and gentamicin/ciprofloxacin was tested by the checkerboard method. The dominant components (> 10%) of the essential oils were: cis-piperitone epoxide and menthone ( C. sylvatica), pulegone and menthone ( C. vardarensis), pulegone and piperitenone ( C. nepeta), pulegone, piperitenone, menthone and piperitone ( C. glandulosa). EOs did not exhibit significant antimicrobial activity except the essential oil of C. vardarensis which was selectively active against Staphylococcus aureus (MIC - 21.25 μg/mL). The overall effect of essential oil-antibiotic combinations varied from synergistic (FICI ≤ 0.5) to antagonistic (FICI ≥ 2) depending on the bacterial strain tested.
Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene
Authors: Sadyrbekov Daniyar , Saliev Timur , Gatilov Yuri , Kulakov Ivan , Seidakhmetova Roza , Seilkhanov Tulegen , Askarova Sholpan
Published: March 2019

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.
Chemical and Genetic Identity of Ligularia tsangchanensis and L. muliensis. Isolation of a Cacalol Precursor from a Hybrid of L. tsangchanensis and L. vellerea
Authors: Kuroda Chiaki , Itoh Taketo , Suzuki Yurika , Suzuki Yuko , Okamoto Yasuko , Tori Motoo , Hanai Ryo , Gong Xun
Published: March 2019

The chemical composition and the DNA sequence of an evolutionarily neutral region were studied for three samples of L. tsangchanensis, three samples of L. muliensis, and one sample of a hybrid between L. tsangchanensis and L. vellerea. L. tsangchanensis and L. muliensis were indistinguishable in the chemical composition and the ITS sequence, supporting a recent taxonomic study that merged the two species into L. tsangchanensis. Cacalol (1) was isolated as the most major component from both L. tsangchanensis and L. muliensis, while 6-acetoxy-9-oxofuranoeremophilane (10), a putative cacalol precursor, was isolated from the hybrid.
Isolation, and Structural Characterization of a New Hexa β-D-Glucopyranosyl Diterpene from Stevia rebaudiana
Authors: Chaturvedula Venkata Sai Prakash , Meneni Srinivasa Rao
Published: March 2019

A new diterpene glycoside with six β-D-glucopyranosyl units was isolated from the extract of the leaves of Stevia rebaudiana Bertoni, which was identified as 13-[(2- O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[2- O-β-D-glucopyranosyl-3- O-(2- O-β-D-glucopyranosyl)-β-D-glucopyrano-syl -β-D-glucopyranosyl) ester (1) based on the extensive NMR (1H and 13C, COSY, HSQC, and HMBC) and mass spectral data as well as hydrolysis studies.
Phytochemistry of Weigela x “kosteriana variegata” (Caprifoliaceae)
Authors: Andriamisaina Nampoina , Mitaine-Offer Anne-Claire , Pruvot Benoist , Chluba Johanna , Miyamoto Tomofumi , Tanaka Chiaki , Lacaille-Dubois Marie-Aleth
Published: March 2019

One new triterpene glycoside 3- O-β-D-xylopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→3)]-β-D-xylopyranosyl-(1→4)-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyloleanolic acid, was isolated from Weigela x “kosteriana variegata” (Caprifoliaceae), with three known ones. Their structures were characterized by a combination of mass spectrometry and 1D and 2D NMR spectrocopic techniques including 1H- and 13C NMR, COSY, TOCSY, NOESY, HSQC, and HMBC experiments. The toxicological properties of some glycosides were determined with a zebrafish-based assay. The results show that the most active compounds were toxic to the larvae in the range of 1 μM.
Characteristic Aromas of Quercus crispula (Japanese Oak)
Authors: Hasegawa Toshio , Mamada Ryosuke , Yamanaka Takahisa , Shimazaki Bujyuro , Fujihara Takashi
Published: March 2019

Nature is important in Japanese culture. Many tree species produce diverse fragrances, making Japan's forests a botanical treasure. However, there have been no detailed investigations of the aroma characteristics of trees, a vital factor in forest bathing. Previously, we applied our analytical method to three tree species found mainly in Japan: Chamaecyparis pisifera, Lindera praecox, and Lindera obtusiloba Blume. In this paper, we report investigations of the aroma of Quercus crispula Blume (Japanese oak) using this analysis method. Japanese oak grows mainly in Japan. The odor compounds of Japanese oak were obtained by hexane extraction and monolithic material sorptive extraction and each extracted compound was identified by gas chromatography olfactometry and gas chromatography-mass spectrometry. Comparison of the aromas and compounds in the extracts resulted in the identification of important aroma compounds in Japanese oak: 10 (including eugenol) in the leaves and 15 (including 3-methyl-4-octanolide) in the wood.
Hairy Root Cultures of Eurycoma longifolia and Production of Anti-inflammatory 9-Methoxycanthin-6-one
Authors: Tran Trang Thu , Nguyen Nam Trung , Pham Ngoc Bich , Chu Huy Nhat , Nguyen Trong Dinh , Kishimoto Tadamitsu , Van Chau Minh , Chu Ha Hoang
Published: March 2019

The content of bioactive alkaloid 9-methoxycanthin-6-one in the roots of Eurycoma longifolia is rather small. In the current study, a high yield of 9-methoxycanthin-6-one was obtained from transgenic hairy roots (1.139 ± 0.20% dry weight, DW) compared to wild roots (0.164 ± 0.25% DW) of E. longifolia. Three liquid basal media supplemented with 3% sucrose were tested for the growth and accumulation of 9-methoxycanthin-6-one. The fastest growth was obtained in the Shenck and Hildebrandt (SH) medium while the highest 9-methoxycanthin-6-one content was observed in McCown's woody plant (WP) medium. In this study, compared to controls, the production of 9-methoxycanthin-6-one was increased by the maximum of 2.6 and 4.0 times after the addition of jasmonic acid and yeast extract, respectively, into hairy root cultures. In addition, pro-inflammatory cytokines IL-6 and TNF-α were significantly inhibited by 9-methoxycanthin-6-one in lipopolysaccharide-stimulated murine RAW264.7 cells, peritoneal macrophages and human THP-1 macrophages These results suggest that the elicited hairy root culture of E. longifolia is an alternative system for the production of an abundant source of anti-inflammatory 9-methoxycanthin-6-one.
Bioactive Metabolites from a Hydrothermal Vent Fungus Aspergillus sp. YQ-13
Authors: Tao Qiannan , Ding Chihong , Auckloo Bibi Nazia , Wu Bin
Published: March 2019

To study fermentation from strain Aspergillus sp. YQ-13, dwelling in sediment Kueishantao hydrothermal vents off Taiwan. Compounds were separated and purified by silica gel column, preparative HPLC techniques. Their structures were identified by the physicochemical properties and spectral analysis. The isolates were identified as 3-hydroxy-2-(2-hydroxy-6-methoxy-4-methylbenzoyl)-5-methoxy-benzoic acid methyl ester (1), myristic acid (2), orcinol (3), 1,2-seco-trypacidin (4), leporin A (5), chaetominine (6), 5-hydroxy-2-hydroxymethyl-4H-pyran-4one (7) and N-methyl-2-pyrolidinone (8). Compound 1 was identified as a new natural product belonging to diphenyl ketone family. Known compounds 5 and 6 exhibited antibiotic activity with MIC value of around 1 to 25 μg mL−1 against Bacillus subtilis, Klebsiella pneumoniae, methicillin resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Acinetobacter Bauman. Compounds 1, 4, 5 and 6 were tested by the methods of DPPH and FRAP assay, showing moderate antioxidant activities.
A New Cytotoxic Tetrahydroxanthene-1,3(2H)-dione Derivative from Uvaria cordata and Structure Revision of Valderramenol A
Authors: Ho Duc Viet , Vo Hung Quoc , Nguyen Tho Huu , Do Thao Thi , Nguyen Hoai Thi
Published: March 2019

A new tetrahydroxanthene-1,3(2H)-dione derivative (1) was isolated from the leaves of Uvaria cordata collected in Viet Nam. Its structure was elucidated to be a mixture of four tautomers (1a–1d) by a combination of extensive spectroscopic analyses and the theoretical calculation of Gibbs free energies. Compound 1 exhibited moderate cytotoxicity against KB, LNCaP, Hep-G2, MKN-7, SW-480, HL-60, and SK-Mel-2 cancer cell lines with IC50 values ranging from 25.92 ± 2.33 to 44.29 ± 4.36 μg/mL. In addition, the previously reported structure of valderramenol A has been revised to 1a/1b.
Synthesis of Novel 2-Thioxothiazolidin-4-one and Thiazolidine-2, 4-dione Derivatives as Potential Anticancer Agents
Authors: Kumar Alleni Suman , Kumar Rathod Aravind , Reddy Elala Pravardhan , Satyanarayana Vavilapalli , Kashanna Jajula , Reddy Boggu Jagan Mohan , Reddy Basireddy Venkata Subba , Yadav Jhillu Singh
Published: March 2019

A variety of novel thiazolidine derivatives (2-thioxothiazolidin-4-one and thiazolidine-2, 4-dione derivatives) have been prepared by using 2,4-diphenyl-2 H-chromene-3-carbaldehyde and its derivatives as starting materials. This is the first example of the preparation of thiazolidine derivatives through this novel method. Structure evolution of the resulting thiazolidine derivatives leads to anticancer agents. Our preliminary data for some model compounds on three cancer cell lines (MCF7, A549 and B-16) suggested reasonable anticancer activity against the A549 and B-16 cell lines, with IC50 values of 20.7 and 20.4 μM, respectively. This method is operationally simple and works with a diverse range of substrates.
A Short Step Conversion of Alkynyl Propargyl Sulfones into Six-Membered Cyclic β-Ketosulfones via an Amine-Induced Novel Ring Closure
Authors: Alam Md. Ashraful , Shimada Kazuaki , Kamoto Hironobu , Shingo Kasumi , Korenaga Toshinobu
Published: March 2019

Alkynyl propargyl sulfones underwent secondary amine-induced cyclization to give six-membered cyclic enaminosulfones, and the products were subjected to acidic hydrolysis to afford the corresponding cyclic β-ketosulfones in high yields.
Oleuropein-Rich Olive Leaf Extracts May Ameliorate Consequences of Glucose-Induced Oxidative Stress in Hep G2 Cells
Authors: Amidžić Mirjana , Marić Patricia , Fumić Barbara , Petlevski Roberta , Bljajić Kristina , Končić Marijana Zovko
Published: March 2019

Olive leaf is reputed to have many beneficial health-related properties, often attributed to the iridoid glycoside oleuropein. In this work, the composition and antioxidant activity of oleuropein-rich olive leaf extracts prepared by ultrasonication were investigated. In addition, the influence of the extracts on Hep G2 cells subjected to glucose-induced oxidative stress was examined. The prepared extracts acted as antioxidants rich in oleuropein and other polyphenols. Among them, the extract prepared at 20°C using 100% ethanol was the most effective radical scavenger, antioxidant and metal chelator. It also contained the largest amount of oleuropein and other phenolic constituents. The extract prepared at 20°C using 70% ethanol, on the other hand, showed the best yield among the investigated extracts. Furthermore, it was able to preserve the cell-membrane integrity of Hep G2 cells subjected to glucose-induced oxidative stress, as well as to maintain intracellular concentration of glutathione and the activity of glutathione S-transferase on the levels comparable to those observed in non-stressed cells. The presented results demonstrate that oleuropein-rich olive leaf extracts can effectively ameliorate the consequences of glucose-induced oxidative stress in liver cells.
Manumycin-type Derivatives from a Streptomyces sp. Associated with Meson Bee Osmia cornifrons
Authors: Wang Zhe , Peng Ai-Hong , Lu Dan-Dan , Song Ya-Jie , Wang Cheng-Lei , Xie Wei-Dong
Published: March 2019

Two new manumycin-type derivatives named as cornifronin A and cornifronin B, and one known analogue antibiotic SW-B were isolated from the liquid fermentation of Streptomyces sp. OC1401 obtained from body surface of a meson bee Osmia cornifrons. The structures of all isolates were identified by spectroscopic methods (HRESIMS, IR, 1D and 2D NMR) and comparison with literature data. The antibacterial and antifungal activities of these manumycin-type derivatives were evaluated by disc diffusion method and more accurate data on the antibacterial activity were obtained through the MIC (Minimum Inhibitory Concentration) and MBC (Minimal bactericidal concentration) values.
The Effects of Aldose Reductase Inhibitor Quercetin and Monochloropivaloylquercetin in Amyloid β Peptide (1–42) Induced Neuroinflammation in Microglial Cells
Authors: Cumaoğlu Ahmet , Yerer Mükerrem Betül
Published: March 2019

Microglial over-activation plays a crucial roles during neuroinflammation. Aldose reductase (AR) is one of the enzymes that has been linked to inflammatory processes in several diseases. Therefore, inhibition of AR is considered as an important strategy to reduce inflammation. In the present study, Quercetin (Q) and monochloropivaloylquercetin (MCPQ) showed potent inhibition on AR expression and anti-neuroinflammatory effects in Amyloid β (Aβ) peptide (1–42) induced inflammatory process by inhibiting expression of inflammatory mediators from microglial cells. Furthermore, ablation of AR caused a significant reduction on COX2 expression in Aβ-induced neuroinflammation. Q and MCPQ suppressed COX2 mRNA and protein expression, which further resulted in downstream inhibition of prostaglandin E2 (PGE2) release in Aβ-induced neuroinflammatory process. Additionally, Aβ treatment resulted in activation of Mitogen Activated Protein Kinase (MAPK) and increased translocation of Nuclear Factor Kappa B (NFκB). Q and Sorbinil significantly reduced the activation of MAPK, at the same time Q, MCPQ and sorbinil decreased nuclear translocation of NFκB and diminished tumor necrosis factor (TNF)-α release in Aβ-induced neuroinflammation. The results suggested that AR is a probable target for treatment of neuroinflammation as well as Q and MCPQ could be effective agents for treating or preventing inflammation-related neurodegenerative diseases by AR inhibition.
Chemical Constituents from the Root Bark of Calophyllum inophyllum
Authors: Sichaem Jirapast , Tip-Pyang Santi , Siripong Pongpun
Published: March 2019

A new chromanone derivative, namely caloinophyllin B (1), along with seventeen known compounds 2–18 were isolated from the root bark of Calophyllum inophyllum. The structures of all isolated compounds (1–18) were determined by spectroscopic methods as well as comparison with previous reports in the literature. To the best of our knowledge, compounds 1, 3, 6, 8, 11–14 and 16 were isolated from this plant for the first time. Moreover, all isolated compounds (1–18) were evaluated for their cytotoxicity against KB and Hela S-3 cell lines. Compound 8 exhibited moderate activity toward KB and Hela S-3cells with the IC50 values of 25.8 and 18.1μM, respectively.
The Extract of Edible Alga Petalonia binghamiae Suppresses TGF-β1-or H2O2-Induced Liver Fibrogenesis in LX-2 and HepG2 Cells
Authors: Choi Young-Ji , Yu Kang-Yeol , Kim Mi-Hee , Kwon Bora , Park In-Sun , Choo Young-Moo , Kim Seon-Young , Jeong Seung-Il , Kim Jiyoung , Kim Ju
Published: March 2019

Although the edible alga Petalonia binghamiae is extensively consumed as a health-promoting food by Northeast Asians, little is known about the biomedical efficacies of P. binghamiae. In this report, we investigated the novel efficacy of P. binghamiae extract ( Pb-E01) using LX-2 and HepG2 cells. Pb-E01 inhibited TGF-β1-induced cell proliferation and gene expression in LX-2 cells. In addition, Pb-E01 reduced H2O2–induced reactive oxygen species (ROS) and alanine aminotransferase (ALT) activity in HepG2 cells. According to these results, we suggest that Pb-E01 plays a functional role in suppressing liver fibrogenesis.
Composition and Chemical Variability of Root Bark oil from Ivoirian Cleistopholis patens
Authors: Ouattara Zana A. , Yapi Thierry A. , Békro Yves-Alain , Mamyrbékova-Békro Akhanovna J. , Paoli Mathieu , Tomi Pierre , Casanova Joseph , Bighelli Ange , Tomi Félix
Published: March 2019

The chemical composition of root bark oil of Cleistopholis patens (Benth.) Engl. & Diels from Côte d'Ivoire was determined by GC (in combination with retention indices), GC-MS and 13C NMR. The contents of the major compounds varied drastically from sample to sample: patchoulenone (0–70.5%), β-pinene (0–51.9%), bornyl acetate (0.5–31.2%), α-pinene (0.2–25.7%), juvenile hormone III (0.3–22.2%) and β-elemol (0–18.8%). Three chemical compositions may be differentiated, dominated by i) patchoulenone, ii) β-pinene and α-pinene, iii) juvenile hormone III, accompanied by bornyl acetate or β-elemol. 13C NMR data of patchoulenol (3-patchoulen-5- exo-ol) are reported.
Chemical Compositions and Tyrosinase Activity of the Essential Oils of Alpinia aquatica
Authors: Romes Nissha Bharrathi , Basar Norazah , Sirat Hasnah M. , Hashim Siti Ernieyanti , Asim Zaini
Published: March 2019

The essential oils obtained from the fresh rhizome, leaf, and pseudostem of Alpinia aquatica Roscoe. were hydrodistillated and analyzed using capillary gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS). β-Pinene (11.7%), α-humulene (8.9%), aromadendrene (8.7%), and sabinene (7.7%) were the major components in the rhizome oil. The most abundant components of the leaf oil were germacrene D (21.3%), β-pinene (15.6%) and sabinene (12.1%), while α-humulene (19.8%), germacrene D (15.2%) and β-caryophyllene (8.7%) were the main constituents in the pseudostem oil. Antityrosinase assay revealed that all the three essential oils exhibited weak tyrosinase inhibition activities. The rhizome oil showed the highest inhibition activity with the value of 9.5% for the L-DOPA oxidation.
Antibacterial Activity of Geraniol in Combination with Standard Antibiotics against Staphylococcus aureus, Escherichia coli and Helicobacter pylori
Authors: Bhattamisra Subrat Kumar , Kuean Chew Hui , Chieh Lee Boon , Yan Vivian Lee Yean , Lee Chin Koh , Hooi Lee Peng , Shyan Lai Pei , Liew Yun Khoon , Candasamy Mayuren , Sahu Priyadarshi Soumyaranjan
Published: March 2019

The antibacterial activity of geraniol and its effect in combination with ampicillin, amoxicillin and clarithromycin against Staphylococcus aureus, Escherichia coli and Helicobacter pylori was tested. The minimum inhibitory concentrations (MICs) and combinatory effects of geraniol against the bacteria were assessed by using the modified broth microdilution and checkerboard assay, respectively. The combinatory effect is expressed as fractional inhibitory concentration index (FICI). The MIC of geraniol against S. aureus, E. coli and H. pylori was found to be 11200, 5600, and 7325 μg/mL, respectively. A significant synergistic effect was observed with geraniol and ampicillin against S. aureus with FICI in the range 0.19 to 0.32. Geraniol and ampicillin exhibited a partial synergistic effect against E. coli. A similar effect was observed with geraniol and clarithromycin against S. aureus. A partial synergistic effect was observed with clarithromycin and geraniol against H. pylori with the FICI value in the range 0.86 to 0.89. An additive effect was observed with geraniol and amoxicillin combination against H. pylori. However, the amoxicillin and clarithromycin dose was reduced by thirty-two fold when combined with geraniol against H. pylori. The anti- H. pylori effect of geraniol with clarithromycin and amoxicillin could be of potential interest in the treatment of H. pylori infection and associated ulcers in humans. Further, geraniol, in combination with other antibiotics, has substantial therapeutic potential against S. aureus and E.coli infection.
Eremophilane Sesquiterpenoids and Nor- and Dinorsesquiterpenoids from Ligularia virgaurea Collected in China
Authors: Nakashima Katsuyuki , Hoshiyama Kie , Hayami Chika , Sakaoku Misato , Okamoto Yasuko , Kuroda Chiaki , Gong Xun , Tori Motoo
Published: March 2019

Four samples of Ligularia virgaurea collected in China afforded three new eremophilane sesquiterpenoids, one new norsesquiterpenoid, and two new dinorsesquiterpenoids as well as 28 known compounds. All the four samples belonged to the ligularol type (L type), one of the five chemotypes we have described for this species. The configuration of the previously reported eremophila-7(11),9-dien-12,8-olide is revised to be H-8α. The absolute configuration of ethyl 8-oxoeremophila-7(11),9-dien-12-oate was established to be 4 S,5 R by a DFT calculation.
A New Flavanone from Seeds of Psoralea corylifolia with α-Glucosidase Inhibitory Activity
Authors: Liu Ying , Dong Jia-Yue , Ren Bo
Published: March 2019

A new flavanone (2 S)-7,4'-hydroxyl-6-(2″,3″-epoxy-3″-methylbutyl)flavanone (1), together with five known compounds (2–6) were isolated from EtOAc-soluble extract of seeds of Psoralea corylifolia. Their structures were elucidated on the basis of spectroscopic and physico-chemical analyses. All compounds were evaluated for in vitro inhibitory activity against α-glucosidase. Among them, new compound 1 was found to exhibit inhibitory activity on α-glucosidase with IC50 value of 29.2 μM.
Phenolic Acid LC/MS Profile of Chenopodium rubrum and Evaluation of Cytotoxic Activity
Authors: Mynarski Adam , Pietrzak Wioleta , Galanty Agnieszka , Dawiec Emilia , Nowak Renata , Podolak Irma
Published: March 2019

The phenolic acids content of methanolic extracts before and after acidic hydrolysis of different morphological parts of Chenopodium rubrum was determined by LC-ESI-MS/MS. Overall eleven phenolic acids were detected and quantified. The most common were p-coumaric, ferulic and salicylic acids, which were present in all analyzed extracts. Gallic, caffeic, vanillic, syringic and sinapic acids were found in bound forms. Gentisic acid was most abundant in roots (3.7 mg/100g d.w.), syringic acid in stalks (2.9 mg/100 g d.w.), ferulic acid in leaves (6.0 mg/100g d.w.), and protocatechuic acid in seeds (2.3 mg/100g d.w.). The highest concentration of examined acids was found in leaves (19.1 mg/100g d.w.) and roots (10.0 mg/100g d.w.). Cytotoxic activity of extracts was tested against human cancer cell lines HTB 140, WM 793, Du 145 and normal PNT2 cells. Only the seed extract showed activity against malignant melanoma WM 793 cell line (69.6 ± 0.7% of dead cells at 100 μg/mL).
Stereoselective Total Synthesis of (6S)-5,6-Dihydro-6-[(2R)-2-hydroxy-6-phenylhexyl]-2H-pyran-2-one from L-Malic Acid
Authors: Chandrakanth Dandekar , Rao Yerragorla Gopala , Swamy Tallapally , Biradar Dhanraj O , Vishnupriya Lonavath , Reddy Basi V Subba
Published: March 2019

A stereoselective total synthesis of an antiproliferative and antifungal natural product, (6 S)-5,6-dihydro-6-[(2 R)-2-hydroxy-6-phenylhexyl]-2 H-pyran-2-one has been accomplished using the Barbier allylation, LiAlH4/LiI mediated syn-stereoselective reduction and olefin ring-closing metathesis (RCM) as the key steps. L-Malic acid was used as a chiral precursor for the construction of syn-1,3-diol moiety of the molecule.
Chemical Composition and Antifungal Activity of Lavender (Lavandula stoechas) Oil
Authors: Özcan Mehmet Musa , Starovic Mira , Aleksic Goran , Figueredo Gilles , Juhaimi Fahad Al , Chalchat Jean-Claude
Published: March 2019

The essential oil of Lavandula stoechas was examined by GC and GC-MS. Discs (5 mmi.d.) of the tested fungi ( Alternaria alternata, Fusarium oxysporum and Botritys cinerea) were inoculated separately onto each assay plate and incubated at 25oC for 7 days. The oil yield of dried parts (v/dw) obtained by hydro-distillation was 2.9%. Thirty-two compounds representing 98.3% of the essential oil were determined. Linalool (49.9%), linalyl acetate (14.4%), lavandulyl acetate (5.7%), α-terpineol (5.6%), terpinene-4-ol (5.1%), lavandulol (3.7%), ( E)-β-ocimene (2.6%) and ( Z)-β-ocimene (2.4%) were identified as the main constituents of the oil. In addition, both doses of the lavender oil showed varying levels of inhibitory effects on the mycelial growth of tested fungi used in the experiment. The results demonstrated the strongest effect on B.cinerea, followed by A.alternata and F.oxysporum. The inhibitory effect is probably dependent on the concentration of essential oils.
Volatile Components of Some Selected Turkish Liverworts
Authors: Asakawa Yoshinori , Baser K. Husnu Can , Erol Bercu , Von Reuß Stephan , Konig Wilfried A. , Ozenoglu Hatice , Gokler Isa
Published: March 2019

The distribution of liverworts and hornworts in Turkey has been described. The volatile components of the ether extracts of some selected Turkish, Spanish and French liverwort species, Corsinia coriandrina, Lejeunea lamacerina, Lunularia cruciata, Marchantia polymorpha, Metzgeria furcata, Pellia neesiana, Porella cordaeana, Porella platyphylla and Scapania undulata were analysed by GC-MS. C. coriandrina produces isothiocyanates and S,S-dimethyliminodithiocarbonates similar to those isolated from the marine hydroid, Tridentata marginata. The species also produces the characteristic 2-arylbenzofurans. L. lamacerina is closely related chemically to the Japanese Lejeunea aquatica, L. flava and L. japonica since it elaborates the same cuparene sesquiterpenoids as those found in the latter three species.
Biotransformation of Bicyclic Sesqui- and Diterpene 1,2-dials and Their Derivatives by the Fungus, Aspergillus niger
Authors: Asakawa Yoshinori , Sekita Masako , Hashimoto Toshihiro
Published: March 2019

Microbial biotransformation of naturally occurring pungent sesquiterpene 1,2-dials, polygodial and cinnamodial, and a diterpene 1,2-dial, sacculatal as well as their tetrahydro derivatives was carried out by using Aspergillus niger. The pungent polygodial and sacculatal are toxic against A. niger not to produce any metabolites while A. niger biotransformed cinnamodial to the lactonized products in small amount. On the other hands, the dihydroxy derivatives of the former two dialdehydes were bioconverted by the same fungus to give hydroxy-, oxo-, carboxylic- and epoxy-products. The stereostructures of each metabolite and their metabolic pathways were described.
New Oxygenated Steroid from the Marine-Derived Fungus Aspergillus flavus
Authors: Yang Meng-Yue , Yang Jian-Kun , Yang Jin-Kai , Hu Lian-Dong , Zhu Hua-Jie , Cao Fei
Published: March 2019

One new oxygenated steroid, named aspersteroid A (1), and three known analogues (2–4) were isolated from the marine-derived fungus Aspergillus flavus collected from the Bohai Sea. Their structures were elucidated by spectroscopic analyses and by comparison with previously reported data. The absolute configuration of tetracyclic nucleus in 1 was assigned by quantum chemical calculation of the electronic circular dichroism (ECD) spectrum. All the compounds were evaluated for their cytotoxic and antibacterial activities.
Sulfated Glycosides from the Sea Cucumbers Block Ca2+ Flow in Murine Neuroblastoma Cells
Authors: Pislyagin Evgeny A. , Menchinskaya Ekaterina S. , Aminin Dmitry L. , Avilov Sergey A. , Silchenko Alexandra S.
Published: March 2019

Recently the ability of triterpene glycosides from sea cucumbers to interact with purinergic receptors of P2X family on the membranes of immune cells enhancing the reversible ATP-dependent Ca2+ intake and therefore modulating (stimulating) the receptor's activity was discovered. As a result the activation of cellular immunity on the organism level was observed. Purinergic receptors are associated with a series of important functions in normal and pathophysiology, thus the searching of its new modulators (both stimulants and blockers) seems to be very important medical and scientific task. The cytotoxic activity of four triterpene glycosides isolated from different species of sea cucumbers: liouvilloside A (1) from Pseudocolochirus violaceus, fallaxoside D1 (2) from Cucumaria fallax and magnumosides C3 (3) and C4 (4) from Massinium (=Neothynidium) magnum against neuroblastoma Neuro 2A cells was tested. The glycosides 1 and 4 were the most cytotoxic in the series (EC50 5.0 μM and 21.5 μM, correspondingly). Moreover, non-cytotoxic concentration (1 μM) of these compounds inhibited ATP-dependent Ca2+ intake into the Neuro 2A cells, containing P2X7 receptors on the surface, by 53 and 47%, correspondingly. The ability of sea cucumbers triterpene glycosides to block the purinergic receptors in vitro is first discovered.
Leaves of Eugenia brasiliensis Used as a Folk Medicine Contain Cyclooxygenase Enzyme and Lipid Peroxidation Inhibitory Compounds
Authors: Dametto Alessandra C. , Boralle Nivaldo , Zhang Chuan-Rui , Silva Dulce H. S. , Nair Muraleedharan G.
Published: March 2019

Tea prepared from leaves of Eugenia brasiliensis has been used as a folk medicine in Brazil to alleviate inflammation and pain. In this study, tea and methanolic extract inhibited cyclooxygenase (COX-1 and -2) enzymes and lipid peroxidation (LPO). Therefore, bioassay-guided purification of methanolic extract was carried out and afforded a new lignan ( aR,8S,8'R-7,8,7’,8'-tetrahydro-8,8'-dimethyl-3,3'-dimethoxy-4,4'-dihydroxy-dibenzocyclooctadiene) and three known C-methylated flavonoids (stropopinin; demethoxymatteucinol; myrigalone G). All isolates at 37.5 μg/mL inhibited COX enzymes, similar to aspirin and ibuprofen used as controls at 108 and 12 μg/mL, respectively. Most of the isolates also showed strong LPO inhibition at 5μg/mL.
The Chaenomeles sinensis Extract has the Potential to Exhibit Antioxidant Activity or Attenuate Liver Damage
Authors: Choi Young-Ji , Choo Young-Moo , Jeong Seung-Il , Yu Kang-Yeol , Kim Jiyoung
Published: March 2019

Even though Chaenomeles sinensis is extensively used as a medicinal product to cure coughs, little is known about the biomedical efficacies of C. sinensis. In this study, we report the novel efficacy of C. sinensis extract ( Cs-E01). Our 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and superoxide anion scavenging assays revealed that Cs-E01 exhibits strong antioxidant activity in vitro. In addition, Cs-E01 played a negative role in transforming growth factor (TGF-β1)-induced gene expression in LX-2 cells, suggesting that Cs-E01 is related to the cellular pathway suppressing liver damage.
An Analysis of Volatile Components of the Liverworts Dumortiera hirsuta subsp. hirsuta and Dumortiera hirsuta subsp. nepalensis (Dumortieraceae) from Panama and Taxonomic Observations on the Species
Authors: Durant-Archibold Armando A. , Allen Noris Salazar , Garrido Anette , Ledezma Jose Gudiño , Gupta Mahabir P.
Published: March 2019

We report for the first time the chemical composition of volatile components (VOCs) of two subspecies, D. hirsuta subsp. hirsuta and D. hirsuta subsp. nepalensis, of the liverwort Dumortiera hirsuta from Panama by using headspace-solid phase microextraction-gas chromatography-mass spectrometry in order to assess distinguishing markers between the two species. Forty VOCs were identified in total for both subspecies. Of these, 34 are reported for the first time in D. hirsuta. Furthermore, both subspecies showed clear differences in the type and amount of VOCs. The major compounds in D. hirsuta subsp. hirsuta were α-gurjunene, β-selinene, α-guaiene, α-humulene and β-caryophyllene; while in D. hirsuta subsp. nepalensis were ledene, α-gurjunene, β-caryophyllene and α-guaiene, respectively. Two oxygenated sesquiterpenes, globulol and nerolidol, could be considered as possible distinguishing chemical markers between these two subspecies. We conclude that both morphotypes of D. hirsuta are chemically different.
Seasonal Study of Methyleugenol Chemotype of Ocimum campechianum Essential Oil and Its Fungicidal and Antioxidant Activities
Authors: Figueiredo Pablo Luis B. , Silva Sebastião G. , Nascimento Lidiane D. , Ramos Alessandra R. , Setzer William N. , da Silva Joyce Kelly R. , Andrade Eloisa Helena A.
Published: March 2019

Ocimum species (Lamiaceae) have aroused great interest to the scientific community with respect to different biological properties attributed to their essential oils. The seasonal variation and antioxidant and fungical activities were carried out for the essential oil of Ocimum campechianum. Its essential oil showed an excellent yield (0.5–5.3%) throughout the season. The essential oils of leaves/stems and inflorescences were analyzed by GC and GC-MS to identify their volatile constituents and associate them with the antioxidant and antifungal activities. Methyleugenol was the main component in the leaves/stems (80.0-87.0%) and inflorescences (75.3–83.5%). The essential oil and the methyleugenol standard showed low antioxidant activity (<40%) against DPPH radical, but high antifungal activity against Fusarium oxysporum and Colletotrichum gossypii phytopatogens as well as the mycelial growth and spore germination of the fungi. The high levels of methyleugenol coupled with good fungicidal activity give great agroindustrial potential to this Ocimum species.