bluuebunny/crossref_metadata_2025 huggingface dataset:
FIGURE 2 from Identification and Characterization of a Small Molecule Bcl-2 Functional Converter
Authors: Kopparapu Prasad R. , Pearce Martin C. , Löhr Christiane V. , Duong Cathy , Jang Hyo Sang , Tyavanagimatt Shanthakumar , O'Donnell Edmond F. , Nakshatri Harikrishna , Kolluri Siva K.
Published: March 2024

BFC1108 suppresses viability of multiple cancer cell types. A, Bcl-2–selective inhibition of clonogenic survival of MDA-MB-231 cells. MDA-MB-231/Vector and MDA-MB-231/Bcl-2 cells were exposed to 10 µmol/L concentration in medium containing 10% FBS for 48 hours and the colony formation was determined after 2 weeks. B, Quantification of colony formation data shown in A. ***, P < 0.001. C, Bcl-2–dependent induction of apoptosis by BFC1108. MDA-MB-231 cells with high or low Bcl-2 expression were treated with 10 µmol/L BFC1108 in a medium containing 10% FBS for 48 hours and apoptosis was determined by annexin V staining. Data from one representative experiment is shown. Refer to Supplementary Fig. S1 for additional independent experiments. D, BFC1108 inhibits viability of breast cancer cells with Bcl-2 expression. The indicated breast cancer cells were treated for 48 hours, and viability was measured using CellTiter-Glo assay. Data were analyzed by two-way ANOVA and Dunnett multiple comparison post hoc test, **, P < 0.001; ****, P < 0.00001. Bottom panel indicates Bcl-2 RMA-normalized mRNA expression from the Cancer Cell Line Encyclopedia (50). E, BFC1108 suppresses viability of multiple cancer cell types with minimal effects on MCF10A nontransformed breast epithelial cells. Cells were treated for 48 hours in 10% serum containing medium and cell viability was determined. ***, P < 0.001.
Supplementary Table 8 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma
Authors: Chai Annie Wai Yeeng , Yee Shi Mun , Lee Hui Mei , Abdul Aziz Norazlin , Yee Pei San , Marzuki Marini , Wong Ka Wo , Chiang Alan K.S. , Chow Larry Ka-Yue , Dai Wei , Liu Teng Fei , Tan Lu Ping , Khoo Alan Soo Beng , Lo Kwok Wai , Lim Paul V.H. , Rajadurai Pathmanathan , Lightfoot Howard , Barthorpe Syd , Garnett Mathew J. , Cheong Sok Ching
Published: March 2024

Genes affected by substantial copy number variation in NPC268
Supplementary Table 2 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma
Authors: Chai Annie Wai Yeeng , Yee Shi Mun , Lee Hui Mei , Abdul Aziz Norazlin , Yee Pei San , Marzuki Marini , Wong Ka Wo , Chiang Alan K.S. , Chow Larry Ka-Yue , Dai Wei , Liu Teng Fei , Tan Lu Ping , Khoo Alan Soo Beng , Lo Kwok Wai , Lim Paul V.H. , Rajadurai Pathmanathan , Lightfoot Howard , Barthorpe Syd , Garnett Mathew J. , Cheong Sok Ching
Published: March 2024

List of 211 published EBV genomes used in this study
Supplementary Figure 6 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma
Authors: Chai Annie Wai Yeeng , Yee Shi Mun , Lee Hui Mei , Abdul Aziz Norazlin , Yee Pei San , Marzuki Marini , Wong Ka Wo , Chiang Alan K.S. , Chow Larry Ka-Yue , Dai Wei , Liu Teng Fei , Tan Lu Ping , Khoo Alan Soo Beng , Lo Kwok Wai , Lim Paul V.H. , Rajadurai Pathmanathan , Lightfoot Howard , Barthorpe Syd , Garnett Mathew J. , Cheong Sok Ching
Published: March 2024

Correlation analyses of BCL2L2 gene expression with its inhibitors.
Supplementary Figure 2 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma
Authors: Chai Annie Wai Yeeng , Yee Shi Mun , Lee Hui Mei , Abdul Aziz Norazlin , Yee Pei San , Marzuki Marini , Wong Ka Wo , Chiang Alan K.S. , Chow Larry Ka-Yue , Dai Wei , Liu Teng Fei , Tan Lu Ping , Khoo Alan Soo Beng , Lo Kwok Wai , Lim Paul V.H. , Rajadurai Pathmanathan , Lightfoot Howard , Barthorpe Syd , Garnett Mathew J. , Cheong Sok Ching
Published: March 2024

EBV in NPC268 can be induced into lytic phase using various chemical inducers.
Comprehensive Treatment of a Rare Case of Complete Primary Pachydermoperiostosis with Large Facial Keloid Scars: A Case Report and Literature Review
Authors: Zhao Haibo , Zhang Jianglin , He Renliang , Bao Linlin
Published: March 2024

Introduction: Pachydermoperiostosis (PDP), or primary hypertrophic osteoarthropathy, is a rare autosomal dominant disease with primary clinical features of pachydermia (thickening of skin) and periostosis (new bone formation). Keloid scar formation is also rather obscure, and some scientists have claimed that keloid scars contain an excessive amount of fibroblasts compared with normal skin as well as a dense mass of irregularly deposited connective tissues. Case Presentation: A 25-year-old man exhibited extensive skin folding on his face, a gyrus-like scalp, depressed nasolabial folds, and keloids. Symptoms began at 18 years of age, progressing insidiously. Additionally, he experienced clubbing of fingers and toes, joint pain, muscle soreness, and hyperhidrosis. Radiographic examinations revealed thickened bone and cystic regions. Diagnosed with complete primary PDP and facial keloid scars, he underwent skin dermabrasion, biopsies, and a comprehensive treatment involving, botulinum toxin injections, 5-fluorouracil, and a carbon dioxide lattice laser. Conclusion: PDP presents challenges due to its unclear etiology but stabilizes over time in most cases. Comprehensive treatment strategies, including dermabrasion and a combination of intralesional therapies, are effective in managing keloids in PDP patients. This case contributes to the understanding of managing rare diseases and underscores the importance of personalized approaches to improve therapeutic outcomes in patients with complete primary PDP and concurrent keloids.
A Review of the Evolving Role of Radiotherapy in the Treatment of Neuroendocrine Neoplasms
Authors: Yit Ling Fung Nelson , Li Youquan
Published: March 2024

Background: Neuroendocrine neoplasms (NENs) are rare tumours that develop from neuroendocrine cells in various parts of the body. The management of this disease poses a significant challenge because of the heterogeneous clinical presentation and varying degrees of aggressiveness. A multidisciplinary approach is often required in complex clinical situations. Radiotherapy (RT) plays a key role in managing NETs in both curative and palliative settings. Summary: In this review, we summarize and discuss recent developments in the field of advanced RT in early-stage, locally advanced, and metastatic NENs. We highlight limitations in current approaches and discuss future potential treatment strategies for patients with NENs.
Influence of Implementing an Opioid Stewardship Team in the Primary Care Setting
Authors: Kelsh Shelby , de Voest Margaret , Stout Michael
Published: March 2024

Background: The Updated 2022 Centers for Disease Control (CDC) Clinical Practice Guideline for Prescribing Opioids for Pain highlights the importance of shared decision making and provider-patient relationships. Interprofessional teams may be helpful in supporting providers and patients. A large, multi-site primary care department created an interprofessional primary care opioid stewardship team to target high-risk opioid prescribing and improve practice alignment with CDC recommendations through dashboard reporting and education. Objective: The primary objective was to assess reduction in morphine milligram equivalents (MME) from baseline to 6 months in patients on opioid doses ≥90 MME daily. The secondary objective assessed change in number of naloxone prescriptions from baseline to 6 months after education. Methods: The study was conducted across 30 primary care sites of one health system within Michigan from 2021 to 2022. The opioid stewardship team included 2 physicians, 3 pharmacists, a project operations manager, and IT support. Interventions included creation of a dashboard, provider education, dissemination of policy, and chart audits. Using the electronic health record (EHR) dashboard, patients on chronic opioid doses ≥90 MME daily or missing an active naloxone prescription were identified. Primary care providers (PCP) were provided with an individual list of patients for whom to consider intervention. Support was provided for prescribers, but the team did not interact with patients directly. Results: Baseline analysis identified 290 patients on doses ≥ 90 MME daily. There was reduction in median daily MME from baseline to 6 months in the overall study population (140 [105 240] vs 120 [90 240], P < .001). At 6 months 181 (62.4%) of patients had been given a prescription for naloxone versus 108 (37.2%) who had one at baseline, P < .001. Conclusion: The initiatives implemented by the opioid stewardship team resulted in statistically significant reductions in MME and an increase in naloxone prescribing from baseline to 6 months post-education.
Effects of silver nanowires and their surface modification on electromagnetic interference, transport and mechanical properties of an aerospace grade epoxy
Authors: Özkutlu Demirel Merve , Öztürkmen Mahide B , Savaş Müzeyyen , Mutlugün Evren , Erdem Talha , Öz Yahya
Published: March 2024

The aerospace industry has progressively grown its use of composites. Electrically conductive nanocomposites are among important modern materials for this sector. We report on a bulk composite containing silver nanowires (AgNW) and an aerospace grade epoxy for use in carbon fiber reinforced polymers (CFRPs). AgNWs’ surfaces were also modified to enhance their ability to be dispersed in epoxy. Composites were obtained by use of three-roll milling which is of major interest for industrial applications, especially for the aerospace sector, since the process is scalable and works for aerospace grade resins with high curing temperatures. Our main objective is to improve the electromagnetic interference (EMI) shielding performance of CFRPs via improving the properties of the resin material. The addition of AgNWs did not considerably alter the flexural strength of the epoxy, however the composite with surface-modified AgNWs has a 46 % higher flexural strength. Adding AgNWs over a low threshold concentration of 0.05 wt% significantly enhanced the electrical conductivity. Conductivities above the percolation threshold lie around 102 S/m. At a concentration of 5 wt% AgNW, the EMI shielding efficiency (SE) of epoxy increased from 3.49 to 12.31 dB. Moreover, the thermal stability of the epoxy was unaffected by AgNWs. As a result, it was discovered that (surface modified) AgNWs improved the (multifunctional) capabilities of the aerospace grade epoxy resin which might be used in CFRPs to further enhance properties of composites parts, demonstrating suitability of AgNWs’ as a reinforcement material in aerospace applications.
A Hijab-Effect Too? Clients’ Reflections on Professionalism and Empathy Toward Hijab-Wearing Public Servants
Authors: Dinhof Katharina , Willems Jurgen , de Boer Noortje
Published: March 2024

Religious symbols, such as the hijab, are often deemed undesirable or banned in public employment. We test if clients’ perceptions and their performance are influenced by a hijab-wearing public servant, and further test if clients’ reflections on empathy or professionalism about the public servant mitigate potential negative effects. We preregistered and conducted a two-step 2 × 3 between-subjects experiment ( n = 2,680; representative sample in Austria). We find no evidence that the wearing of a hijab by a public servant negatively influences clients’ perceptions, nor their performance during a public service process. The reflection answer with respect to professionalism or empathy, however, is related to clients’ performance: Clients’ positive reflection on public servants’ empathy or professionalism—independent of whether the public servant wears a hijab or not—positively relates to their performance in terms of task correctness. We discuss the relevance of these results regarding religious stereotyping and public employment policies.
Association Between Augmentation Index and Total Sleep Time in Night Shift Workers
Authors: Gusmão Waléria D. P. , Silva Victor M. , Paiva Annelise M. G. , Mota-Gomes Marco Antonio , Nadruz Wilson , Moreno Claudia R. C.
Published: March 2024

Augmentation index and pulse wave velocity are markers of vascular compromise and independent predictors of cardiovascular risk and mortality. While the link between shift work and heightened cardiovascular risk is established, the intricate genesis of early cardiovascular outcomes in shift workers remains incompletely understood. However, there is evidence that sleep duration plays a role in this regard. Here we evaluate the association of total sleep time with pulse wave velocity, augmentation index, and central blood pressure in night shift workers. This study cross-sectionally evaluated the association of total sleep time evaluated by 10-day monitoring actigraphy with augmentation index, pulse wave velocity, and brachial and central blood pressure evaluated by oscillometry in nursing professionals, 63 shift workers (89% women; age = 45.0 ± 10.5 years), and 17 (100% women; age = 41.8 ± 15.6) day workers. There were no differences in the studied variables between shift workers and day workers. Results of correlation analysis demonstrated that pulse wave velocity, central systolic blood pressure, central diastolic blood pressure, brachial systolic blood pressure, and brachial diastolic blood pressure tended to have significant correlation with each other, while these measures did not have a significant relationship with augmentation index in both groups. However, results of adjusted restricted cubic spline analysis showed a U-shaped-curve association between total sleep time and augmentation index ( p < 0.001 for trend) with a nadir at 300-360 min of total sleep time in shift workers. The present study showed that total sleep time, assessed by actigraphy, had a U-shaped association with augmentation index in shift workers, which indicated better characteristics of vascular functionality when sleep time was 5-6 h in the workers studied.
Economic Abuse by An Intimate Partner and Its Associations with Women’s Socioeconomic Status and Mental Health
Authors: Mellar Brooklyn M. , Fanslow Janet Lynn , Gulliver Pauline J. , McIntosh Tracey K. D.
Published: March 2024

What is the prevalence and impact of economic abuse by an intimate partner in the population? Does experience of economic abuse compound the effects of other intimate partner violence (IPV) types on women’s mental health and financial wellbeing? This study used a population-based and representative sample of 1,431 ever-partnered New Zealand women to explore associations between their experience of economic abuse and a range of mental health and financial outcomes. Logistic regression was conducted, and Adjusted Odds Ratios (AORs) were reported. Overall, 15% of ever-partnered women experienced any economic abuse, with the most prevalent act “refused to give money for household expenses,” reported by 8.8% of the sample. Women who experienced economic abuse presented increased risk for poor mental health (AORs ranging from 2.59 for poor mental health to 4.89 for having a diagnosed health mental health condition) and financial insecurity outcomes (AORs ranging from 3.09 for receiving government benefits to 4.72 for experiencing food insecurity) compared with women who experienced no IPV or women who had experienced any IPV (physical, sexual, psychological or controlling behavior) excluding economic abuse. Findings suggest that economic abuse may compound effects of IPV and highlight the importance of acknowledging and addressing economically abusive behaviors and their long-term detrimental impact on women’s mental health and financial security. Implementing wider forms of safety planning that address issues of economic independence and security, and social support are needed to augment plans that focus on physical safety.
Fatigue life and transmission efficiency prediction of marine timing gears under 3D mixed lubrication state
Authors: Sun Wen , Shi Xiujiang , Lu Xiqun , Hua Deliang , Li Renze , Liu He , Wu Gang
Published: March 2024

The timing gears are the core transmission components of marine diesel engine, whose working conditions are complex and harsh, transient and coupled with the microscopic lubrication state, meshing in the mixed lubrication environment with lubrication-contact coexistence, local asperity contact brings stress concentration, further affects the fatigue life and friction loss. In this study, based on a three-dimensional (3D) line-contact mixed lubrication model, dynamic subsurface stress calculation, fatigue life prediction, and friction loss power study were carried out on timing gears, taking into account transient operating conditions and actual surface roughness. For the established simulation model, an equivalent simulation test of line-contact friction of timing gears was carried out, which can realize the measurement of friction coefficient under the condition of different slip-roll ratios, and make up for the deficiency that the traditional test equipment can only carry out the test under the fixed slip-roll ratio. Although the gear meshing process can only be simulated by the disc specimen due to the limitation of the specimen form, this limitation is similar to the simplified form of the simulation model, which can meet the needs of the verification test. The influence of structure and material on the lubrication state, fatigue life and friction power consumption are studied, which provide theoretical guidance for the tribological optimization design and reliability analysis for the marine timing gears.
Controlling recast layer and material removal in reverse µEDM for sustainable fabrication of microelectrode arrays
Authors: Pratap Suresh , Datta Somak , Sharma Vinay
Published: March 2024

Microelectrode arrays (MEAs) are essential for researching neurons and heart tissue because they record and stimulate neuronal activity. For manufacturing MEAs on conductive materials, reverse microelectrical discharge machining, or reverse µEDM, presents a viable method. However, achieving precise MEAs requires control over recast layers generated during micromachining. This study focuses on recast layer thickness (RLT) and material removal rate (MRR) as key performance indicators for sustainable reverse µEDM fabrication of MEAs. RLT affects surface stability, while MRR influences energy consumption and cost. Optimising these responses depends on three input factors: feed rate, capacitance, and voltage. ANOVA analysis reveals that capacitance is crucial in both MRR and recast layer thickness (RLT) in reverse µEDM, contributing to 88.71% and 94.04% simultaneously. A hybrid artificial neural network-genetic algorithm for global optimisation was employed. The optimised parameters 46.5nF capacitance, 110 V voltage, and 5 µm/sec feed yielded a minimum RLT of 1.58 µm and a maximum MRR of 0.3 mm³/min. These advancements signify significant improvements in the precision and efficiency of MEAs fabrication through reverse µEDM, contributing to its sustainable implementation.
Investigation of micro-electro-discharge machining process parameters during machining of M2 hardened die-steel
Authors: Pradhan B B , Tiwary A P , Masanta M , Bhattacharyya B
Published: March 2024

Micro-electro-discharge machining (micro-EDM) is a well-known micromachining method to create microstructures on hard-to-cut materials. To further exploit the capability of micro-EDM, the present research work investigates the effect of various important micro-EDM process parameters on the material removal rate (MRR), tool wear rate (TWR), and overcut (OC) during micro-cavity machining of M2-hardened die-steel. A Taguchi experimental design was used to study the effects of three process parameters namely pulse-on-time (Ton), peak current (Ip), and gap voltage (Vg). The experimental results showed that the most influencing process parameter in the present experimental investigation is peak current, Ip followed by pulse-on-time, Ton and gap voltage, Vg. Furthermore, a recent and effective method i.e. Measurement of Alternatives and Ranking according to COmpromise Solution (MARCOS) has been successfully employed to determine optimal process parametric combination for micro-EDM process to achieve maximum MRR and minimum TWR and OC during machining of micro-cavity on M2-hardened die-steel. From the present research investigation, the optimal micro-EDM process parametric combinations obtained for higher MRR, lower TWR and OC are found as 1 A of peak current, 30 μs pulse-on time and gap voltage of 60 V. Furthermore, through various scanning electron microscope micrographs of micro-cavities and micro-machined surfaces, analysis has been done to find out the micro-cavity with best geometrical accuracy and surface feature to validate the best and most ideal combinations of micro-EDM input parameters for micromachining operations. The results of this study show that micro-EDM is a promising process for machining M2-hardened die-steel. The optimization of process parameters using the MARCOS methodology can further improve the machining efficiency and accuracy of micro-EDM.
Experimental verification of a translation model of linear transfer function of ultrasonic vibrations with nonlinear wave modulation for detection of contact-type failures
Authors: Tanaka Takashi , Tamura Takato , Oura Yasunori
Published: March 2024

Contact-type failures, such as the crack closure at the tip of a fatigue crack, closed delamination of composite materials, and loosening of bolted joints, are difficult to detect through nondestructive inspections using linear ultrasonic waves or vibrations. When low-frequency vibrations, such as environmental disturbances, operating vibrations, and vibrations excited by actuators, are applied to a structure with contact-type failure, the contact condition of the failure surface fluctuates in synchronization with the low-frequency vibration. The amplitude and phase of ultrasonic waves or vibrations through contact-type failure with fluctuating contact conditions are modulated in synchronization with low-frequency vibrations, that is, realizing nonlinear wave modulation. Previous reports have presented evaluation and localization methods of contact-type failure using amplitude and phase demodulation signals. In this study, amplitude and phase demodulations were affected by the change in the viscous damping of a structure. Therefore, the essence of nonlinear wave modulation was considered to be the fluctuation of the natural frequencies. In this study, a visualization experiment of the fluctuation of natural frequencies caused by nonlinear wave modulation was conducted. The time-varying transfer function was reconstructed by measuring the amplitude and phase demodulation signals with the change in the excitation frequency. Moreover, the translation model of the transfer function of the ultrasonic vibration with nonlinear wave modulation was evaluated.
Safety and efficacy of the yellow sub-threshold micropulse laser for uveitic macular edema: A pilot study
Authors: Bellucci Carlo , Bruni Francesco , Radice Lorenzo Marco , Tedesco Salvatore Antonio , Rossi Maurizio , Gandolfi Stefano , Mora Paolo
Published: March 2024

Purpose

To assess the safety and efficacy of the yellow sub-threshold micropulse laser (YSML) in eyes with uveitic macula edema (UME).

Methods

A prospective interventional study. Eyes with non-infectious UME and an unsatisfactory response to prior conventional treatments underwent navigated YSML (NAVILAS®). The treatment was planned on an imported macular OCT scan. The central macular thickness (CMT) and best-corrected visual acuity (BCVA) were recorded immediately before the treatment (baseline) and again after 1 and 3 months. The end of the follow up (FU) was the last available evaluation in the case of no UME recurrence; or when the CMT increase required rescue therapy.

Results

Eight eyes of as many patients were included. The mean duration of the FU was 252 days (range: 170–398 days). No adverse events were observed. In the whole cohort the mean values improvement over time was significant for both CMT ( p = 0.011) and BCVA ( p < 0.001). The indication for a new treatment was stated in 4 eyes (50%) after a mean FU of 277 ± 43 days. This time was statistically comparable with the mean FU of eyes without UME recurrence. At the baseline the non-UME recurrence eyes had a significantly lower mean CMT (326 ± 80 vs 485 ± 112 μm, p = 0.03).

Conclusions

The application of YSLM for UME has not previously been mentioned in literature. Our results were promising in terms of both safety and efficacy. This would authorize further studies on the procedure, also as a first line treatment in naif UME cases.
Crashworthiness design of novel CFRP functionally-graded conical square structures with variable circumscribed diameters
Authors: Xiong Feng , Wen Jiaxin , Wang Dengfeng , Gui Liangjin , Jiang Yanjun , Wu Hang
Published: March 2024

Carbon fiber reinforced plastic (CFRP) thin-walled structures have been widely acknowledged as superior energy absorbers due to comprehensive advantages on crashworthiness and light weight. However, few studies have been reported concerning CFRP thin-walled structures with nonlinearly variable sectional dimensions along the longitudinal direction. Hence, in the present study, a novel CFRP functionally-graded conical square thin-walled structure with variable circumscribed diameter (functionally-graded conical square tube, FGCST) is proposed and comprehensively investigated. Specifically, finite element (FE) models of FGCST under multi-angle crushing loading are first built and validated. Second, a comprehensive crashworthiness comparison among the FGCST, the conventional CFRP conical square tube (CST) and the CFRP straight square tube (SST) under multi-angle crushing loading is executed to explore the relative merits of FGCST over others. Third, a parameter study is implemented on FGCST to explore the action laws of its structural parameters on its overall crashworthiness under multi-angle crushing loading. Finally, the FGCST is multi-objective crashworthiness optimized for maximizing overall specific energy absorption (SEAθ) and minimizing overall peak crushing force (PCF0) simultaneously through Taguchi method coupled with grey relational analysis (GRA). The optimized FGCST obtains 6.06% higher of SEAθ and meanwhile 40.58% lower of PCF0 compared with the baseline design, thus demonstrating good potential as a superior candidate for vehicle collision energy absorbers.
Systemic immune-inflammation index as a novel hematological marker for predicting the recurrence of deep venous thrombosis
Authors: Donmez Ibrahim , Muduroglu Ayhan
Published: March 2024

Objective

To determine whether there was a possible predictive relationship between systemic immune-inflammation index (SII) and recurrence of deep venous thrombosis (DVT).

Methods

A total of 231 patients with DVT who met the study criteria and whose data could be accessed were enrolled to this retrospective single-centered cross-sectional study. Of them 26 patients with DVT recurrence consisted of the study group (Group 1) while remaining 205 cases without recurrence were considered as the control population (Group 2). The patients’ basic clinical features and laboratory results from the complete blood count (CBC) test were recorded and compared between groups. Following univariate analyses, a multivariate logistic regression analysis was performed to identify the independent predictors of the recurrence of DVT. Additionally, a receiver-operating characteristic (ROC) curve analysis was performed to detect the cut-off values of the predictors with sensitivity and specificity rates.

Results

There were no significant differences between the groups for basic clinical features, except for diabetes mellitus, pulmonary embolism, and atrial fibrillation. Although the univariate analysis revealed that the median values of NLR, PLR, and SII were significantly higher in the DVT recurrence group, only SII was determined to be a significant and independent predictor of DVT recurrence in the multivariate logistic regression analysis. According to ROC curve analysis, SII of 1685 × 103/mm3 constituted the cut-off value for predicting DVT recurrence with 61.5% sensitivity and 76.6% specificity (AUC = 0.686, p = .001).

Conclusion

The present study demonstrated for the first time in the literature that SII significantly predicted the recurrence of DVT.
Trismus as the Initial Presentation of Anti-Hu Paraneoplastic Neurological Syndrome
Authors: Mitarnun Witoon , Apiwattanakul Metha , Chindavech Narin , Sombat Benyapha , Jantapatsakun Suttanon , Sornnuwat Pheeracha
Published: March 2024

A 73-year-old man presented with subacute trismus and pancerebellar dysfunction. Brain imaging and routine blood test results were unremarkable. Chest computed tomography revealed an indistinctly enhancing 4.7 × 2.5 × 1.8-cm3 pulmonary mass in the right upper lung, with enlarged right paratracheal and hilar lymph nodes. Biopsy of the right supraclavicular lymph node confirmed metastatic carcinoma, with differential diagnoses of small cell carcinoma and poorly differentiated carcinoma, indicating lung cancer as the primary source. Paraneoplastic immunohistochemistry screening revealed anti-Hu antibodies in the serum at a titer of 1:7680 (normal range <1:240) and in the cerebrospinal fluid (CSF) at a titer of 1:256 (normal range <1:2). The line blot method yielded positive results for anti-Zic4 antibodies in serum, with a titer of >1:10 (normal range <1:10), whereas CSF anti-Zic4 was negative (normal range <1:2). The patient developed non-responsive hospital-acquired pneumonia and respiratory failure, and discharged himself against medical advice. This rare case indicates that trismus can be an initial manifestation of anti-Hu paraneoplastic neurological syndrome, and emphasizes the importance of clinical awareness.
The Analysis of Intermolar Width and Skeletal Base Class as a Predictor of Potential Maxillary Canine Impaction in Permanent Dentition: A Cross-Sectional Study
Authors: Dost Hawa , Ehsan Ambreen Afzal , Sakrani Hasnain , Munir Sundus , Lal Abhishek , Ahmed Naseer , Marya Anand , Heboyan Artak
Published: March 2024

Objectives. This study aimed to analyze the inter-molar width and skeletal base class to predict maxillary canine impaction in permanent dentition. Methods. This cross-sectional study was carried out between September 2021 to March 2022. Patients aged between 13 and 20 years were recruited. These patients’ cone-beam computer tomography(CBCT), lateral cephalogram, and dental casts were collected and analyzed. For statistical analysis, T-test and Chi-square tests were applied. Results. A total of 92 patients were enrolled in this study. Inter-molar width was significantly decreased in patients with impacted canine( P < .001). The skeletal base classification was statistically significant when compare with intermolar width( P < .05). The location of impaction ( P = .060), type of impaction( P = .435), and side of impaction( P = .239) were determined to be statistically insignificant. Conclusion. The Inter-molar width was decreased in patients with impacted canine; therefore, it may be a potential risk factor. The skeletal classification was found to affect inter-molar width in impacted canine patients.
Beyond Failure and Success: A Process View on Imperfect Projects as Common Practice
Authors: Marrewijk Alfons van , Stjerne Iben , Sydow Jörg
Published: March 2024

This editorial scrutinizes the dichotomy of a project’s success and failure, which, in our opinion is too rigid, inflexible, and unnuanced. The aim of this special issue is to nuance this dichotomy by moving toward a process view on how imperfection is brought about in projects. We introduce and discuss five topics important for such a process view: (1) improvisation, (2) temporality, (3) power and politics, (4) transition, and (5) intentionality. We argue that a holistic, processual view of imperfections premises emergence and continuous learning and judgments of the project both in and over time. All five articles in this special issue deal with at least one of the discussed themes of our proposed process view on imperfect projects.
Inhibition of Drp1- Fis1 interaction alleviates aberrant mitochondrial fragmentation and acute kidney injury
Authors: Song Zhixia , Xia Yao , Shi Lang , Zha Hongchu , Huang Jing , Xiang Xiaohong , Li Huiming , Huang Hua , Yue Ruchi , Wang Hongtao , Zhu Jiefu
Published: March 2024

Background

Acute kidney injury (AKI) is a common clinical disorder with complex etiology and poor prognosis, and currently lacks specific and effective treatment options. Mitochondrial dynamics dysfunction is a prominent feature in AKI, and modulation of mitochondrial morphology may serve as a potential therapeutic approach for AKI.

Methods

We induced ischemia–reperfusion injury (IRI) in mice (bilateral) and Bama pigs (unilateral) by occluding the renal arteries. ATP depletion and recovery (ATP-DR) was performed on proximal renal tubular cells to simulate in vitro IRI. Renal function was evaluated using creatinine and urea nitrogen levels, while renal structural damage was assessed through histopathological staining. The role of Drp1 was investigated using immunoblotting, immunohistochemistry, immunofluorescence, and immunoprecipitation techniques. Mitochondrial morphology was evaluated using confocal microscopy.

Results

Renal IRI induced significant mitochondrial fragmentation, accompanied by Dynamin-related protein 1 (Drp1) translocation to the mitochondria and Drp1 phosphorylation at Ser616 in the early stages (30 min after reperfusion), when there was no apparent structural damage to the kidney. The use of the Drp1 inhibitor P110 significantly improved kidney function and structural damage. P110 reduced Drp1 mitochondrial translocation, disrupted the interaction between Drp1 and Fis1, without affecting the binding of Drp1 to other mitochondrial receptors such as MFF and Mid51. High-dose administration had no apparent toxic side effects. Furthermore, ATP-DR induced mitochondrial fission in renal tubular cells, accompanied by a decrease in mitochondrial membrane potential and an increase in the translocation of the pro-apoptotic protein Bax. This process facilitated the release of dsDNA, triggering the activation of the cGAS-STING pathway and promoting inflammation. P110 attenuated mitochondrial fission, suppressed Bax mitochondrial translocation, prevented dsDNA release, and reduced the activation of the cGAS-STING pathway. Furthermore, these protective effects of P110 were also observed renal IRI model in the Bama pig and folic acid-induced nephropathy in mice.

Conclusions

Dysfunction of mitochondrial dynamics mediated by Drp1 contributes to renal IRI. The specific inhibitor of Drp1, P110, demonstrated protective effects in both in vivo and in vitro models of AKI.
Draft genome of Brasenia schreberi, a worldwide distributed and endangered aquatic plant
Authors: Wu Lin-Fang , Zhu Wei-Guang , Yu En-Ping , Cao Hong-Lin , Wang Zheng-Feng
Published: March 2024

Objectives

Brasenia is a monotypic genus in the family of Cabombaceae. The only species, B. schreberi, is a macrophyte distributed worldwide. Because it requires good water quality, it is endangered in China and other countries due to the deterioration of aquatic habitats. The young leaves and stems of B. schreberi are covered by thick mucilage, which has high medical value. As an allelopathic aquatic plant, it can also be used in the management of aquatic weeds. Here, we present its assembled and annotated genome to help shed light on medial and allelopathic substrates and facilitate their conservation.

Data description

Genomic DNA and RNA extracted from B. schreberi leaf tissues were used for whole genome and RNA sequencing using a Nanopore and/or MGI sequencer. The assembly was 1,055,148,839 bp in length, with 92 contigs and an N50 of 22,379,495 bp. The repetitive elements in the assembly were 555,442,205 bp. A completeness assessment of the assembly with BUSCO and compleasm indicated 88.4 and 90.9% completeness in the Eudicots database and 95.4 and 96.6% completeness in the Embryphyta database. Gene annotation revealed 67,747 genes that coded for 73,344 proteins.
De novo genome assembly of a high-protein soybean variety HJ117
Authors: Liu Zhi , Yang Qing , Liu Bingqiang , Li Chenhui , Shi Xiaolei , Wei Yu , Guan Yuefeng , Yang Chunyan , Zhang Mengchen , Yan Long
Published: March 2024

Objectives

Soybean is an important feed and oil crop in the world due to its high protein and oil content. China has a collection of more than 43,000 soybean germplasm resources, which provides a rich genetic diversity for soybean breeding. However, the rich genetic diversity poses great challenges to the genetic improvement of soybean. This study reports on the de novo genome assembly of HJ117, a soybean variety with high protein content of 52.99%. These data will prove to be valuable resources for further soybean quality improvement research, and will aid in the elucidation of regulatory mechanisms underlying soybean protein content.

Data description

We generated a contiguous reference genome of 1041.94 Mb for HJ117 using a combination of Illumina short reads (23.38 Gb) and PacBio long reads (25.58 Gb), with high-quality sequence coverage of approximately 22.44× and 24.55×, respectively. HJ117 was developed through backcross breeding, using Jidou 12 as the recurrent parent and Chamoshidou as the donor parent. The assembly was further assisted by 114.5 Gb Hi-C data (109.9×), resulting in a contig N50 of 19.32 Mb and scaffold N50 of 51.43 Mb. Notably, Core Eukaryotic Genes Mapping Approach (CEGMA) assessment and Benchmarking Universal Single-Copy Orthologs (BUSCO) assessment results indicated that most core eukaryotic genes (97.18%) and genes in the BUSCO dataset (99.4%) were identified, and 96.44% of the genomic sequences were anchored onto twenty pseudochromosomes.
Resistance training modifies of serum levels of matrix metalloproteinase 2 and tissue inhibitor of matrix metalloproteinases in multiple sclerosis women - a randomized controlled trail
Authors: Nezhad Nasrin Niazi , Parnow Abdolhossein , Khamoushian Kianoosh , Eslami Rasoul , Baker Julien S
Published: March 2024

The objectives of the present study was to investigate the effects of resistance training (RT) on serum levels of controlling blood-brain barrier (BBB) permeability indices and cognitive performance in MS women (MS-W). In this randomized control trail study (IRCT registration code: IRCT20120912010824N3, 07.09.2023), twenty-five MS-W were randomly divided into sedentary (MS) and resistance exercise (12 weeks/3 times per week/ 60–80% of 1RM) (MS + RT) groups. Fifteen healthy aged-matched women participated as a control group (HCON). The serum level of matrix metalloproteinase-2 (MMP-2), matrix metallopeptidase-9 (MMP-9), tissue metalloproteinase inhibitors-1 (TIMP-1), tissue metalloproteinase inhibitors-2 (TIMP-2), and S100 calcium-binding protein B (S100B) were assessed. In addition, cognitive performance was assessed pre- and post- intervention with the Brief International Cognitive Assessment for MS (BICAMS). A significant reduction in MMP-2, TIMP-2 serum levels, and MMP-2/TIMP-2 ratio were observed in post-test for MS + RT group (p < 0.01) in comparison to the HCON and MS groups; however, no changes were observed in MMP-9, TIMP-1, S100B and MMP-9/TIMP-1 ratio after RT (p > 0.05). The verbal learning was improved in post-test for MS + RT group (p < 0.01), although no change were observed for visuospatial memory and information processing speed (p > 0.05). These findings suggest that resistance training can modify some indices of BBB permeability and improve verbal learning in MS-W. The findings may also be beneficial as a non-pharmacological intervention to reduce inflammation.
Effectiveness of tranexamic acid in burn patients undergoing surgery – a systematic review and meta-analysis
Authors: Slob Joeri , Gigengack Rolf K. , van Baar Margriet E. , Loer Stephan A. , Koopman Seppe S. H. A. , van der Vlies Cornelis H.
Published: March 2024

Background

Reducing blood loss during excisional surgery in burn patients remains a challenge. Tranexamic acid during surgery can potentially reduce blood loss. The use of tranexamic acid during excisional surgery in burn patients has recently been described in a review and meta-analysis. However, quality assessment on studies included was not performed and this review did not apply independent reviewers. Quality assessment of studies investigating the effectiveness of tranexamic acid in burn patients is crucial before concusions can be drawn. Therefore, we conducted a systematic review and meta-analysis of the literature investigating the effectiveness of tranexamic acid in burn patients undergoing surgery.

Methods

A systematic review and meta-analysis of the literature was conducted. The study was pre-registered in PROSPERO database (CRD42023396183).

Results

Five studies including two randomised controlled trials (RCTs) with a total of 303 patients were included. Risk of bias of the included studies was moderate to high. Individual results of the studies were heterogeneous. In three studies of moderate quality the administration of tranexamic acid resulted in a reduction of blood loss per unit excised area, accounting as moderate level of evidence. In two low-quality studies and one moderate quality study the administration of tranexamic acid resulted in a reduction of transfused packed Red Blood Cells (pRBC’s), accounting for moderate level of evidence. Postoperative haemoglobin levels were higher after tranexamic acid administration in one study, accounting for insufficient evidence. Meta-analysis pooling overall blood loss from two separate RCTs failed to detect a statistically significant reduction. Substantial heterogeneity was observed.

Conclusions

Moderate level of evidence indicates that tranexamic acid reduces blood loss per unit of excised area and transfusion of packed Red Blood Cells. Results indicate that tranexamic acid can be beneficial in burn patients undergoing surgery. More high-quality research is needed to confirm these results. Future studies should focus on the dosing of tranexamic acid, the administration approaches, and even consider combining these approaches.

Trial Registration

PROSPERO: CRD42023396183
Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect hepatocellular carcinoma cells from oxidative stress
Authors: Liu Baowang , Dou Jian , Cao Jinglin
Published: March 2024

Background

Cellular response to oxidative stress plays significant roles in hepatocellular carcinoma (HCC) development, yet the exact mechanism by which HCC cells respond to oxidative stress remains poorly understood. This study aimed to investigate the role and mechanism of super enhancer (SE)-controlled genes in oxidative stress response of HCC cells.

Methods

The GSE112221 dataset was used to identify SEs by HOMER. Functional enrichment of SE-controlled genes was performed by Metascape. Transcription factors were predicted using HOMER. Prognosis analysis was conducted using the Kaplan-Meier Plotter website. Expression correlation analysis was performed using the Tumor Immune Estimation Resource web server. NRF1 and SPIDR expression in HCC and normal liver tissues was analyzed based on the TCGA-LIHC dataset. ChIP-qPCR was used to detect acetylation of lysine 27 on histone 3 (H3K27ac) levels of SE regions of genes, and the binding of NRF1 to the SE of SPIDR. To mimic oxidative stress, HepG2 and Hep3B cells were stimulated with H2O2. The effects of NRF1 and SPIDR on the oxidative stress response of HCC cells were determined by the functional assays.

Results

A total of 318 HCC-specific SE-controlled genes were identified. The functions of these genes was significant association with oxidative stress response. SPIDR and RHOB were enriched in the “response to oxidative stress” term and were chosen for validation. SE regions of SPIDR and RHOB exhibited strong H3K27ac modification, which was significantly inhibited by JQ1. JQ1 treatment suppressed the expression of SPIDR and RHOB, and increased reactive oxygen species (ROS) levels in HCC cells. TEAD2, TEAD3, NRF1, HINFP and TCFL5 were identified as potential transcription factors for HCC-specific SE-controlled genes related to oxidative stress response. The five transcription factors were positively correlated with SPIDR expression, with the highest correlation coefficient for NRF1. NRF1 and SPIDR expression was up-regulated in HCC tissues and cells. NRF1 activated SPIDR transcription by binding to its SE. Silencing SPIDR or NRF1 significantly promoted ROS accumulation in HCC cells. Under oxidative stress, silencing SPIDR or NRF1 increased ROS, malondialdehyde (MDA) and γH2AX levels, and decreased superoxide dismutase (SOD) levels and cell proliferation of HCC cells. Furthermore, overexpression of SPIDR partially offset the effects of NRF1 silencing on ROS, MDA, SOD, γH2AX levels and cell proliferation of HCC cells.

Conclusion

NRF1 driven SPIDR transcription by occupying its SE, protecting HCC cells from oxidative stress-induced damage. NRF1 and SPIDR are promising biomarkers for targeting oxidative stress in the treatment of HCC.
Antimicrobial resistance patterns, virulence genes, and biofilm formation in enterococci strains collected from different sources
Authors: Ghazvinian Maryam , Asgharzadeh Marghmalek Saba , Gholami Mehrdad , Amir Gholami Sanaz , Amiri Elham , Goli Hamid Reza
Published: March 2024

Background

Currently, antibiotic-resistant strains of Enterococcus are considered to be one of the critical health challenges globally. This study aimed to investigate the antibiotic susceptibility pattern, biofilm formation capacity, and virulence genes of enterococci isolated from different sources.

Methods

In this cross-sectional study, environmental and fecal samples were collected from the hospital environment, volunteers, and hospital staff from October 2018 to August 2019. The isolates were identified by morphological and biochemical tests (gram staining, catalase, bile resistance, esculin hydrolysis, carbohydrate fermentation, growth in 6.5% NaCl, Pyrrolidonyl arylamidase, arginine dehydrolase), and PCR for ddl gene. An antimicrobial susceptibility test was performed by the standard disk agar diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Quantitative microplate assays were used to assess biofilm production. The bacterial DNAs were extracted by alkaline lysis method and polymerase chain reaction technique was used detect the esp, ace, and efaA virulence genes.

Results

Out of 145 isolates, 84 (57.9%) were identified as E. faecalis and 61 (42.1%) as E. faecium. Resistance to kanamycin and quinupristin-dalfopristin was 82.1% (69/84) and 85.7% (72/84), respectively, in E. faecalis isolates. Out of 61 E. faecalis isolates, 38 (62.4%) were resistant to kanamycin. Among the E. faecalis isolates, esp was the most dominant virulence gene (73.80%), followed by efaA, and ace, which were detected in 60.71%, and 30.95% isolates, respectively. In total, 68.27% of the strains were biofilm producers. Further, esp and efaA genes were more frequently found among E. faecalis strains with moderate and strong biofilm biomass.

Conclusions

According to the findings of our study, enterococci strains isolated from different samples possess distinctive patterns of virulence genes. The esp, ace, and efaA genes were more prevalent among E. faecalis than E. faecium. Besides, the high level antibiotic resistance of normal flora and environmental enterococci strains is alarming the researchers.