bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of genomic regions associated with soybean responses to off-target dicamba exposure
Authors: Canella Vieira Caio , Jarquin Diego , do Nascimento Emanuel Ferrari , Lee Dongho , Zhou Jing , Smothers Scotty , Zhou Jianfeng , Diers Brian , Riechers Dean E. , Xu Dong , Shannon Grover , Chen Pengyin , Nguyen Henry T.
Published: December 2022

The widespread adoption of genetically modified (GM) dicamba-tolerant (DT) soybean was followed by numerous reports of off-target dicamba damage and yield losses across most soybean-producing states. In this study, a subset of the USDA Soybean Germplasm Collection consisting of 382 genetically diverse soybean accessions originating from 15 countries was used to identify genomic regions associated with soybean response to off-target dicamba exposure. Accessions were genotyped with the SoySNP50K BeadChip and visually screened for damage in environments with prolonged exposure to off-target dicamba. Two models were implemented to detect significant marker-trait associations: the Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) and a model that allows the inclusion of population structure in interaction with the environment (G×E) to account for variable patterns of genotype responses in different environments. Most accessions (84%) showed a moderate response, either moderately tolerant or moderately susceptible, with approximately 8% showing tolerance and susceptibility. No differences in off-target dicamba damage were observed across maturity groups and centers of origin. Both models identified significant associations in regions of chromosomes 10 and 19. The BLINK model identified additional significant marker-trait associations on chromosomes 11, 14, and 18, while the G×E model identified another significant marker-trait association on chromosome 15. The significant SNPs identified by both models are located within candidate genes possessing annotated functions involving different phases of herbicide detoxification in plants. These results entertain the possibility of developing non-GM soybean cultivars with improved tolerance to off-target dicamba exposure and potentially other synthetic auxin herbicides. Identification of genetic sources of tolerance and genomic regions conferring higher tolerance to off-target dicamba may sustain and improve the production of other non-DT herbicide soybean production systems, including the growing niche markets of organic and conventional soybean.
The effects of multiwalled carbon nanotubes and Bacillus subtilis treatments on the salt tolerance of maize seedlings
Authors: Luo Ying , Zeng Wenzhi , Lei Guoqing , Hou Yaling , Ao Chang , Chen Haorui , Gaiser Thomas , Srivastava Amit Kumar
Published: December 2022

Nanomaterials, including multiwalled carbon nanotubes (MWCNTs), have been recently applied in agriculture to improve stress resistance, leading to contradictory findings for antioxidant responses and mineral nutrient uptake. A pot experiment involving maize in low-salinity sandy loam soils was conducted with the application of different concentrations (0, 20, 50 mg/L) of MWCNTs and the growth-promoting rhizobacteriumBacillus subtilis(B. subtilis). The dose-dependent effects of MWCNTs were confirmed: 20 mg/L MWCNTs significantly promoted the accumulation of osmolytes in maize, particularly K+in the leaves and roots, increased the leaf indoleacetic acid content, decreased the leaf abscisic acid content; but the above-mentioned promoting effects decreased significantly in 50 mg/L MWCNTs-treated plants. We observed a synergistic effect of the combined application of MWCNTs andB. subtilison plant salt tolerance. The increased lipid peroxidation and antioxidant-like proline, peroxidase (POD), and catalase (CAT) activities suggested that MWCNTs induced oxidative stress in maize growing in low-salinity soils.B. subtilisreduced the oxidative stress caused by MWCNTs, as indicated by a lower content of malondialdehyde (MDA). The MWCNTs significantly increased the leaf Na+content and leaf Na+/K+ratio; however, when applied in combination withB. subtilis, the leaf Na+/K+ratio decreased sharply to 69% and 44%, respectively, compared to those of the control (CK) group, the contents of which were partially regulated by abscisic acid and nitrate, according to the results of the structural equation model (SEM). Overall, the increased osmolytes and well-regulated Na+/K+balance and transport in plants after the combined application of MWCNTs andB. subtilisreveal great potential for their use in combating abiotic stress.
Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage
Authors: Li Yutong , Ma Jizhong , Gao Xueqin , Tie Jianzhong , Wu Yue , Tang Zhongqi , Hu Linli , Yu Jihua
Published: December 2022

Tip-burn has seriously affected the yield, quality and commodity value of mini Chinese cabbage. Calcium (Ca2+) deficiency is the main cause of tip-burn. In order to investigate whether exogenous brassinosteroids (BRs) can alleviate tip-burn induced by calcium (Ca2+) deficiency and its mechanism, in this study, Ca2+ deficiency in nutrient solution was used to induced tip-burn, and then distilled water and BRs were sprayed on leaves to observe the tip-burn incidence of mini Chinese cabbage. The tip-burn incidence and disease index, leaf area, fluorescence parameters (Fv/Fm, NPQ, qP andφPSII) and gas exchange parameters (Tr, Pn, Gs and Ci), pigment contents, cell wall components, mesophyll cell ultrastructure and the expression of genes related to chlorophyll degradation were measured. The results showed that exogenous BRs reduced the tip-burn incidence rate and disease index of mini Chinese cabbage, and the tip-burn incidence rate reached the highest on the ninth day after treatment. Exogenous BRs increased the contents of cellulose, hemifiber, water-soluble pectin in Ca2+ deficiency treated leaves, maintaining the stability of cell wall structure. In addition, BRs increased photosynthetic rate by increasing the activities of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and fructose 1,6-bisphosphatase (FBPase) related to Calvin cycle, maintaining relatively complete chloroplast structure and higher chlorophyll content via down-regulating the expression of BrPPH1 and BrPAO1 genes related to chlorophyll degradation. In conclusion, exogenous BRs alleviated calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis.
Does Fintech affect the psychological traits of managers? Based on the perspective of manager overconfidence
Authors: Wang Liang , Xiao Wenyi , Huang Dequan
Published: December 2022

Overconfidence has undertaken an indispensable role in the psychology of managers and places important significance on managers’ behavior and decision-making. This study investigates the effect of Fintech on the psychological traits of managers from the perspective of overconfidence based on the panel data of Chinese A-share non-financial listed firms and the digital inclusive finance index of Chinese prefecture-level cities between 2011 and 2020. The empirical results show that (1) Fintech exerts a negative effect on manager overconfidence; (2) the main channels of the negative effect of Fintech on manager overconfidence include Fintech coverage breadth and Fintech usage depth; (3) for firms with severe financing constraints and lower power concentration, the negative effect of Fintech on manager overconfidence is more prominent; and (4) our benchmark results still hold after a series of robust tests, including IV regression, altering the measurement of Fintech and manager overconfidence, and employing logit model re-estimation. Based on the above findings, this study provides some insights into the cause for managers’ psychological traits, maintaining managers’ mental health, and empowering the firms’ sustainable development by adopting Fintech.
The role of social exclusion in the formation of suicidal ideation among the elderly in rural pension institutions
Authors: Wang Pingda , Gao Peng , Yu Zehui
Published: December 2022

Introduction

Social exclusion as well as a sense of belonging and depression have been identified as risk factors for suicide among older adults in pension institutions. In particular, the elderly living in rural pension institutions is more likely to have poor mental health and a higher incidence of suicidal ideation. This study explored the mechanism of social exclusion on suicidal ideation among the elderly in rural pension institutions, and the moderating effect of interpersonal trust.

Methods

The social exclusion experience scale, sense of belonging, depression self-rating scale (CES-D), suicidal ideation scale (BSI-CV), and interpersonal trust scale (ITS) were used to investigate the elderly in rural pension institutions. A total of 1,387 samples (53.35% female) were collected, ranging in age from 65 to 95 years (M = 72.8, SD = 6.173).

Results

The results of the study found that: (1) social exclusion increases the suicidal ideation of the elderly in rural pension institutions, and the sense of belonging and depression play a significant chain-mediated role in the relationship between social exclusion and suicidal ideation in the elderly. (2) Interpersonal trust moderates the impact of social exclusion on the sense of belonging, depression, and suicidal ideation. Specifically, interpersonal trust can alleviate the promotion effect of social isolation on suicidal ideation and depression, and can also reduce the adverse effect of social exclusion on the sense of belonging.

Discussion

This study validates that social exclusion is a risk factor for suicidal ideation in the rural elderly and identifies interpersonal trust as a protective factor against social exclusion and its negative outcomes in the elderly. This study provides a scientific basis for improving the depression status of the elderly in rural China and formulating suicide prevention measures.
Analysis of personal psychological motivation and social psychological motivation of Retaliatory Justice under moral public opinion: Chinese cases
Authors: Liu Xi , Zhao Xiaoying , Wang Baomin
Published: December 2022

From a social psychology perspective, this study explored the personal and social psychological motivation of Retaliatory Justice. This study constructed a four-dimensional model of the psychological motivation of Retaliatory Justice from the two dimensions of “Self vs. Society” and “Identity vs. Resource.” They were Identity-Based Self-Oriented Motivation (Pacifying Outrage), Resource-Based Self-Oriented Motivation (Occupying Resource), Identity-Based Society-Oriented Motivation (Value Confirmation), and Resource-Based Society-Oriented Motivation (Deterrence and Control). In this study, 497 sets of valid data were extracted from 6 universities in Xi'an, Shaanxi Province, China, using 3 sets of situational experiments and the “Questionnaire Star” online survey platform as a vehicle to explore the personal motivations and psychosocial motivations of Retaliatory Justice. The empirical results showed that the situational experiment significantly verified the existence of Pacifying Outrage and Value Confirmation, and partially verified the existence of Deterrence and Control and Occupying Resource. Multiple linear regression analysis showed that only the effect of Pacifying Outrage on Retaliatory Justice showed a main effect, and the interaction between Pacifying Outrage and Deterrence and Control was not significant. Pacifying Outrage significantly affects Retaliatory Justice, while Occupying Resource significantly affects Retaliatory Justice, but the interaction between the two was not significant. Value Confirmation significantly affects Retaliatory Justice. Value Confirmation and Deterrence and Control synergistically affect Retaliatory Justice, but Deterrence and Control had no significant effect on Retaliatory Justice. Taking emotions as clues, this paper discussed the realistic value of the rheological paths of the psychological motivation of Retaliatory Justice, which brought enlightenment to the improvement of social morality, the cultivation of judicial trust, and the construction of psychological service system.
Improving social emotional functioning in adolescents with Developmental Language Disorders: A mini review and recommendations
Authors: Arts Elke , Orobio de Castro Bram , Luteijn Ellen , Elsendoorn Ben , Vissers Constance T. W. M.
Published: December 2022

Adolescents with Developmental Language Disorders (DLD) have more difficulties in social emotional functioning than their typically developing peers (TD), such as shyness and anxiety in social situations, fewer peer relations, greater risk of victimization, social isolation and depression. In addition, they are more likely to report higher levels of hyperactivity and conduct problems. These problems derive from a complex interplay between difficulties in language, social communication, underlying cognitive deficits in Theory of Mind (ToM), Executive Functioning (EF) and self-directed speech (SDS). The aim of this mini review is to provide an overview of studies examining the effectiveness of interventions targeting the factors underlying social emotional functioning of school-aged children and adolescents with DLD. We found that studies dedicated to social emotional functioning in school-aged children and adolescents with DLD were relatively scarce. Based on this overview, we give suggestions to improve social emotional functioning in adolescents with DLD. We propose that intervention programs should target the social, linguistic and cognitive functions underlying social emotional functioning and create opportunities to practice these skills in daily, real-life situations with peers.
Effect of the COVID-19 pandemic on the proportion of physically active children and adults worldwide: A systematic review and meta-analysis
Authors: Chaabna Karima , Chaabane Sonia , Jithesh Anupama , Doraiswamy Sathyanarayanan , Mamtani Ravinder , Cheema Sohaila
Published: December 2022

Introduction

Safety measures implemented to address the COVID-19 pandemic have had a profound impact on the mobility of people worldwide We synthesized the global evidence on physical activity (PA) participation before and during the pandemic.

Methods

We conducted a systematic review, searching PubMed, Embase, WHO Global literature on coronavirus disease (between January 2020 and April 2022), and reference lists. Meta-analysis and meta-regression were conducted to quantitatively synthesize the data.

Results and discussion

Sixty-three primary studies were included. In children, the global pooled prevalence of PA was 46.4% before the pandemic, 40.6% during the pandemic before movement restriction (MR), and 19.5% during MR. A statistically significant decrease in prevalence was observed between the period before the pandemic and the period during which MR was implemented (p < 0.001). In adults, the global pooled prevalence (both sexes) decreased between the periods before the pandemic (64.7%) and during MR (57.0%). During the period of COVID-19 MR, children had significantly lower odds to meet the WHO PA recommendation than adults (19.5%, 95%CI: 15.8–23.8% vs. 57.0%, 95%CI: 43.3–62.5%; OR = 0.21; p ≤ 0.001). Patient populations were less active than the general population, and their PA levels decreased during the pandemic. Mental and physical health benefits of PA have been well-demonstrated. Prioritizing PA in health campaigns and strategies is critical to address health issues exacerbated during this pandemic.

Protocol registration

doi: 10.17605/OSF.IO/GVABX.
Health shocks, basic medical insurance and common prosperity: Based on the analysis of rural middle-aged and elderly groups
Authors: Zhang Yuan , Sun Yuquan , Xie Mingli , Chen Yuping , Cao Shouhui
Published: December 2022

Health is a major part of human welfare. The index system of common prosperity was constructed for middle-aged and elderly people in rural areas. Besides, the impart of health shocks and rural basic medical insurance on common prosperity was explored. The data for this study came from China Health and Retirement Longitudinal Survey (CHARLS) in 2013, 2015, and 2018. The finding shows that health shocks hindered the improvement of the common prosperity of the middle-aged and elderly in rural areas, among which daily activities produced the greatest negative effect. The heterogeneity analysis shows that health shocks have a stronger negative effect on the common prosperity of low-income groups than that of high-income ones. The shock of daily activity ability has the greatest influence on the middle-aged and elderly between 45 and 55 years old. However, acute health shocks have a strong negative effect on those aged above 56. The mechanism analysis shows that rural basic medical insurance can alleviate the health shocks to middle-aged and elderly people, but the effect is limited. In general, low-income groups benefit more. Therefore, China should speed up the promotion of the Healthy China Strategy and the reform of the rural basic medical insurance system, and prompt changes from an inclusive to a targeted policy to provide more precise safeguards for vulnerable groups.
The impact of access to sanitary toilets on rural adult residents' health: Evidence from the China family panel survey
Authors: Chen Baoqi , Jin Fulei , Zhu Yaxin
Published: December 2022

Toilet sanitation is related to public health and environmental protection. In the context of the toilet revolution in rural China, an ordered probit regression analysis was conducted to evaluate the impact of access to sanitary toilets on rural residents' health. Using data from the China Family Panel Study (CFPS) in 2014, we found that access to sanitary toilets in rural households significantly improved residents' health, and this finding remained robust across a series of checks. Meanwhile, results of the mechanism analysis showed that preventing feces from contaminating water sources was an important mechanism behind the positive effects of sanitary toilet use on health. We also found that the impact of access to sanitary toilets was more pronounced among female, middle-aged, and low-income people. Toilet revolution plays an important role in ensuring residents' health and protecting water sources, thereby underscoring the need for governments in developing countries to invest in sanitary toilets. In addition, the existing policies and sanitation programs in China need to be improved to promote public health.
Calf circumference has a positive correlation with physical performance among community-dwelling middle-aged, older women
Authors: Wang Po-Chun , Yeh Wei-Chung , Tsai Yi-Wen , Chen Jau-Yuan
Published: December 2022

Introduction

Sarcopenia and frailty are well-known public health problems in middle-aged and older people. Calf circumference (CC) is a representative anthropometric index that may be useful for screening sarcopenia. Physical performance, assessed by hand grip strength and gait speed, measures sarcopenia and frailty. This community-based, cross-sectional study was conducted in Guishan District, Taoyuan City, between April and October 2017 to investigate the relationship between CC and physical performance among community-dwelling middle-aged, older people in Taiwan and to evaluate potential sex differences. CC tends to be an efficient predictor of physical performance in community health screenings and outpatient clinics for community health examinations, where there is limited time for surveys.

Methods

A total of 1,308 volunteers aged 50–85 were recruited. Volunteers who declined to participate, those with recent cardiovascular disease, and those with an inability to complete an interview, physical performance examinations, and body composition measurements were excluded from the study. A total of 828 participants were enrolled in this study (237 men and 591 women). The statistical methods applied in this study were the Mann–Whitney U-test, independent two-sample t-test, Chi-square test, and multivariate logistic regression models.

Result and discussion

Significant differences were observed in age, waist circumference, appendicular skeletal mass index, calf circumference, hand grip strength, and income between men and women. No significant differences were observed between the men and women regarding body mass index, gait speed, exercise habits, or underlying disorders of diabetes mellitus, hypertension, or hyperlipidemia. Comparing across three different CC tertiles, we discovered significant differences in age, body mass index, waist circumference, appendicular skeletal muscle index, gait speed, and hand grip strength in both men and women. On multivariate logistic regression, after adjusting for age, appendicular skeletal mass index, body mass index, exercise habits, income levels, and CC were positively correlated with physical performance as measured by both gait speed (β = 0.15, p = 0.01) and hand grip strength (β = 0.25, p < 0.001) in women, compared to only hand grip strength (β = 0.41, p < 0.001) in men. Lower calf circumference is an independent risk factor for poor physical performance, especially among women.
Even Small Forest Patches Can Help Fight Climate Change!
Authors: Depauw Leen , Meeussen Camille , De Lombaerde Emiel , De Pauw Karen , Verheyen Kris , De Frenne Pieter
Published: December 2022

Climate change is caused by humans emitting polluting gases. Carbon dioxide (CO2) is one of the most important and well-known polluting gases we emit. Forests are carbon vacuum cleaners: via their leaves, trees take up carbon and store it in their wood, roots, and leaves. We found that forest edges, where it is warmer and sunnier, can store more carbon than the forest interior, where it is cooler and shadier. This is important, because the amount of forest edges has increased due to large forests being split up into small forest patches by roads, towns, or agriculture. In Europe, this extra carbon storage in forest edges is not usually considered important, but it represents the equivalent of a forest with an area of more than 1.4 million football (soccer) fields! To maximize carbon storage, we should not only protect small forests, but also plant new forests—even very small ones.
The optimal PEEP after alveolar recruitment maneuver assessed by electrical impedance tomography in healthy horses
Authors: Andrade Felipe Silveira Rego Monteiro , Ambrósio Aline Magalhães , Rodrigues Renata Ramos , Faccó Lara Lopes , Gonçalves Lucas Alaião , Garcia Filho Sérgio Grandisoli , dos Santos Rosana Thurler , Rossetto Thais Colombo , Pereira Marco Aurélio Amador , Fantoni Denise Tabacchi
Published: December 2022

Background

Electrical impedance tomography (EIT) has been an essential tool for assessing pulmonary ventilation in several situations, such as the alveolar recruitment maneuver (ARM) in PEEP titration to maintain the lungs open after atelectasis reversion. In the same way as in humans and dogs, in horses, this tool has been widely used to assess pulmonary aeration undergoing anesthesia, mechanical ventilation, recruitment maneuver, standing horses, or specific procedures.

Objectives

The present study aimed to evaluate the distribution of regional ventilation during ARM based on lung monitoring assessment by EIT, with a focus on better recruitment associated with less or no overdistention.

Methods

Fourteen horses of 306 ± 21 kg undergoing isoflurane anesthesia in dorsal recumbency were used. The animals were mechanically ventilated with a tidal volume of 14 ml kg−1 and a respiratory rate of 7–9. An alveolar recruitment maneuver was instituted, increasing the PEEP by five cmH2O every 5 min until 32 cmH2O and decreasing it by five cmH2O every 5 min to 7 cmH2O. At each step of PEEP, arterial blood samples were collected for blood gas analysis, EIT images, hemodynamic, and respiratory mechanics.

Results

Associated with the CoV-DV increase, there was a significant decrease in the DSS during the ARM and a significant increase in the NSS when PEEP was applied above 12 cmH2O compared to baseline. The ComplROI showed a significant increase in the dependent area and a significant decrease in the non-dependent area during ARM, and both were compared to their baseline values. The driving pressure decreased significantly during the ARM, and Cst, PaO2, and PaO2/FiO2 ratio increased significantly. The VD/VT decreased significantly at DEPEEP17 and DEPEEP12. There was an HR increase at INPEEP27, INPEEP 32, and DEPEEP17 (p < 0.0001; p < 0.0001; and p < 0.05, respectively), those values being above the normal reference range for the species. The SAP, MAP, DAP, CI, and DO2I significantly decreased INPEEP32 (p < 0.05).

Conclusion

The ARM by PEEP titration applied in the present study showed better ventilation distribution associated with better aeration in the dependent lung areas, with minimal overdistention between PEEP 17 and 12 cmH2O decreasing step. Those changes were also followed by improvements in static and regional compliance associated with increased oxygenation and pulmonary ventilation. ARM promoted a transitory decrease in arterial blood pressure and depression in CI with a concomitant drop in oxygen delivery, which should be best investigated before its routine use in clinical cases.
Donor Skeletal Muscle Quality Affects Graft Mortality After Living Donor Liver Transplantation- A Single Center, Retrospective Study
Authors: Tomiyama Takahiro , Harada Noboru , Toshima Takeo , Nakayama Yuki , Toshida Katsuya , Morinaga Akinari , Kosai-Fujimoto Yukiko , Tomino Takahiro , Kurihara Takeshi , Takeishi Kazuki , Nagao Yoshihiro , Morita Kazutoyo , Itoh Shinji , Yoshizumi Tomoharu
Published: December 2022

The recipient muscle status is closely associated with postoperative poor survival in recipients of living donor liver transplantation (LDLT). However, it is uncertain whether LDLT donor muscle quality and quantity affect graft quality. Hence, we analyzed the correlation between donor muscle status and graft function. We measured the skeletal muscle mass index (SMI) and intramuscular adipose tissue content (IMAC) of 380 LDLT donors. We examined the correlation between donor SMI or IMAC and graft mortality, the occurrence rates of small-for-size graft (SFSG) syndrome, and 6-month graft survival rates. The donor SMI had no effect on the occurrence of SFSG syndrome and graft survival, while a high IMAC in both male and female donors was significantly correlated with the rate of SFSG syndrome [high vs low: (male donors) 15.8% vs. 2.5%, p = 0.0003; (female donors) 12.8% vs. 3.1%, p = 0.0234] and 6-month graft survival rates [(male donors) 87.7% vs 95.9%, p = 0.02; (female donors) 83.0% vs. 99.0%, p < 0.0001]. Multivariate analysis revealed that a high donor IMAC (HR; 5.42, CI; 2.13–13.8, p = 0.0004) was an independent risk factor for 6-month graft survival, and the donor IMAC is useful for donor selection for high-risk recipients.
Force Characteristics of Centrifugal Pump as Turbine during Start-Up Process under Gas–Liquid Two-Phase Conditions
Authors: Chai Baodui , Yang Junhu , Wang Xiaohui
Published: December 2022

The start-up process of a centrifugal pump as turbine (PAT) under gas–liquid two-phase conditions was simulated based on Fluent, and the evolution mechanism of the internal flow field and the variation law of force characteristics were studied in its start-up process under gas–liquid two-phase conditions. The results show that the area with high gas phase concentration corresponds to a strong vortex at the beginning of the start-up. The vortex intensity in the impeller gradually decreases with an increase in rotational speed. The gas volume fraction of the blade suction surface is more significant than that of the blade pressure surface. The higher the inlet gas volume fraction (IGVF) is, the more severely the blade load will fluctuate during the start-up process. As the rotational speed increases, the fluctuation of the blade load gradually weakens, and the maximum load is distributed near the inner edge of the blade after the rotational speed is stable. The periodic unbalanced radial force is produced in the start-up process. From the pure liquid conditions to the gas–liquid two-phase conditions with increasing IGVF, the dominant frequency amplitude of radial force shows a similar trend of decreasing first but then increasing. After the rotational speed tends to be stable, the dominant frequency of radial force is equal to the rotational frequency of the blade. With the increase in rotational speed, the dominant frequency amplitude of axial force decreases gradually. The higher the IGVF, the greater the dominant frequency amplitude of axial force at the same time.
Special Issue “11th EASN International Conference on Innovation in Aviation & Space to the Satisfaction of the European Citizens”
Authors: Guadagno Liberata , Pantelakis Spiros , Strohmayer Andreas
Published: December 2022

This Special Issue contains selected papers from works presented at the 11th EASN International Conference on “Innovation in Aviation & Space to the Satisfaction of the European Citizens” (http://easnconference [...]
Isolation, Identification, and Characterization of Endophytic Bacillus from Walnut (Juglans sigillata) Root and Its Biocontrol Effects on Walnut Anthracnose
Authors: Feng Xiaofei , Xu Rong , Zhao Ning , Wang Dongmei , Cun Mengren , Yang Bin
Published: December 2022

Anthracnose is a major disease of walnut, which seriously reduces the yield and quality of walnut in Yunnan province. Therefore, it is necessary to explore and find a biological control agent for the prevention and control of anthracnose disease. In this study, an endophytic Bacillus WB1, with broad-spectrum antibacterial activity was isolated and screened from healthy walnut roots. The strain WB1 was identified as Bacillus siamensis WB1 based on morphological characteristics, physiological and biochemical tests, and 16S rRNA gene sequence analysis. Bacillus siamensis WB1 produces siderophores and indole-3-acetic acid and solubilizes inorganic phosphate. The strain WB1 not only showed a significant inhibition effect on fourteen phytopathogens, but also showed obvious inhibition on the spore germination of Colletotrichum acutatum. Meanwhile, strain WB1 can code genes for the production of antifungal lipopeptides and generate extracellular hydrolytic enzymes (protease, β-1, 3-glucanase, cellulase, and amylase). In addition, WB1 activated the systemic resistance of the host plant by enhancing the activity of defense enzymes, including phenylalanine ammonia lyase (PAL), peroxidase (POD), and polyphenol oxidase (PPO). The results of greenhouse assays also revealed that B. siamensis WB1 can effectively reduce the occurrence and severity of walnut anthracnose disease. These results also indicated that B. siamensis WB1 is a potential biocontrol agent for walnut anthracnose.
Optimal Design and Experiment of Critical Components of Hand-Pushing Corn Plot Precision Planter
Authors: Zhao Huihui , Zhang Dongxing , Yang Li , Cui Tao , Song Wei , He Xiantao , Wu Hailiang , Dong Jiaqi
Published: December 2022

The mechanized sowing operation of corn breeding can improve test accuracy and efficiency and speed up the test process. In view of the problems of low flexibility, high price, and poor sowing quality of the current seeder in corn breeding experiments, the critical components of a hand-pushing corn plot precision planter were optimized. Through the analysis of the relative motion between corn seed and the hole seed-metering wheel, the hole diameter range and hole depth range of the hole seed-metering wheel were determined as 13~19 mm and 6~12 mm, respectively. The chamber structure was optimized to improve the seed filling performance under the condition of a small number of seeds. The angle range between the connecting plate and the horizontal line (ACP) and the angle range between the back plate and the horizontal line (ABP) were 35~55° and 40~70°, respectively. EDEM was used to simulate the seeding circumstances of hole seed-metering wheel with different holes sizes. The optimal combination of hole diameter and depth under different seeds’ sizes and the appropriate angle combination of ACP and ABP were obtained through the orthogonal test. The results of the simulation experiment showed that the optimal hole diameter–depth combinations of the small, medium, and large corn seeds were 13–8, 15–10, and 17–8 mm, respectively. The angle ACP and the angle ABP were, respectively, 45°and 70°. The soil bin test was carried out to further verify the actual seeding effect and the influence of the rotating speed of hole seed-metering wheel on seeding. The results showed that when the rotating speed of hole seed-metering wheel was less than 16.0 r/min, the qualified seeding rates and the double seeds rates of the small, medium, and large corn seeds were higher than 90% and 85%, respectively. The missing seeding rates were 0%. The variation coefficients of plant spacing were less than 5%, indicating good stability of plant spacing, which met the design requirements. The study results can provide a theoretical reference for the optimization design of maize breeding equipment in a plot.
Comparative Fruit Morphology and Anatomy of Wild Relatives of Carrot (Daucus, Apiaceae)
Authors: Kadluczka Dariusz , Grzebelus Ewa
Published: December 2022

Fruit morphological and anatomical characteristics are essential in the taxonomy of Apiaceae. Daucus L. is one of the most important genera of the family Apiaceae, as it contains the cultivated carrot, a crop of great economic importance, and about 40 wild species that could serve as potential sources of genetic diversity for crop improvement. However, the taxonomic and phylogenetic relationships among these species have not yet been fully clarified. In this study, we comparatively investigated the fruit morphology and anatomy of 13 Daucus taxa and four closely related non-Daucus species using light and scanning electron microscopy to evaluate the taxonomic value of these characteristics. A wide range of variations was observed in the fruit morpho-anatomical characteristics across the taxa and revealed several diagnostically valuable features, thus proving to be taxonomically useful. For Daucus, the observed differences included the fruit size (2.1–8.4 mm), shape (from ellipsoid to oblong), and weight (0.079–1.349 g/100 fruits), as well as the fruit surface sculpturing and some anatomical characteristics, i.e., the presence/absence and size of vittae, the shape and size of vascular bundles, and the shape of exocarp cells. This study contributes to a better understanding of the relationships among the genus Daucus.
Assessing the Representativeness of Irrigation Adoption Studies: A Meta-Study of Global Research
Authors: Pathak Ruchie , Magliocca Nicholas R.
Published: December 2022

For decades, nations around the world have been promoting irrigation expansion as a method for improving agricultural growth, smoothing production risk, and alleviating rural poverty. Despite its apparent advantages, suboptimal adoption rates persist. According to the existing literature, determinants of irrigation adoption are often highly dependent on cultural, contextual, and/or local institutional factors. Yet, studies from diverse geographies identify a consistent set of factors. Thus, to be able to make generalizable inferences from such studies, a global geographic representativeness assessment of irrigation adoption studies was conducted to determine whether identified factors influencing irrigation were the result of geographic, epistemological, or disciplinary biases. The results indicate that multiple geographic biases exist with respect to studying farmers’ irrigation adoption decision-making. More research on this topic is being conducted in regions that have little to a high percentage of irrigation (>1%), are readily accessible, receive moderate amounts of average annual rainfall, and have moderate amounts of cropland cover. The results suggest the need to expand research efforts in areas with little to no irrigation to identify constraints and help accelerate economic growth, poverty reduction, and food and livelihood security for rural communities in these regions.
RNA-Interference-Mediated Aphid Control in Crop Plants: A Review
Authors: Zhang Jiahui , Li Huiyuan , Zhong Xue , Tian Jinfu , Segers Arnaud , Xia Lanqin , Francis Frédéric
Published: December 2022

Crop plants suffer severe yield losses due to the significant damages caused by aphids. RNA interference (RNAi) technology is a versatile and environmentally friendly method for pest management in crop protection. Transgenic plants expressing siRNA/dsRNA and non-transformative methods such as spraying, microinjection, feeding, and a nanocarrier-delivery-mediated RNAi approach have been successfully applied for agricultural insect pest management. In this review, we summarize the application of host-induced gene silencing (HIGS)-mediated RNAi, spray-induced gene silencing (SIGS)-mediated RNAi, and other delivery-method-mediated RNAi methods for aphid control. We further discuss the challenges in RNAi application and propose potential solutions to enhance RNAi efficiency.
Labour Commutation in the Agricultural Sector—An Analysis of Agricultural Workers in Chile
Authors: Mancilla Claudio , Ferrada Luz María , Soza-Amigo Sergio , Rovira Adriano
Published: December 2022

Agricultural activity is a key strategic sector for countries, particularly for food production. One way to strengthen the sector is through the use of technology. However, while integrating new technologies, challenges have emerged in the sector with regard to incorporating workers. Thus, one way to resolve this workforce shortfall could be through labour commutation, which consists of people living in one territory but working in another. Therefore, this study aims to analyse the likelihood of agricultural workers becoming worker commuters. Chile is used as a case study, drawing on the Chilean National Employment Survey of 2018 to analyse differences among the five largest regions in the country: Great North, Small North, Central Zone, South, and Chilean Patagonia. Each zone possesses different characteristics, and a propensity score matching process was carried out within the data. The results indicate that agricultural workers with a higher education level, full-time jobs, working in the formal sector, and younger workers are more likely to commute. Different impacts of variables were found across the zones. However, most of the variables have similar impact on the likelihood to commute in different areas. Thus, from a labour perspective, public policies can create general incentives to attract a new workforce to strengthen the agricultural sector.
Interactive Effects of Soil Water, Nutrients and Clonal Fragmentation on Root Growth of Xerophilic Plant Stipa breviflora
Authors: Fan Ruyue , Lv Shijie , Ding Yong , Li Qingfeng
Published: December 2022

Root traits are often used to predict the ecological adaptations of plants. Water and nutrient availability together with fragment size are likely to affect the adaptative capacity of Stipa breviflora and help plants spread and explore new sites, while the effects of water, nutrients and fragment size on S. breviflora’s root traits have rarely been studied in combination. Here, a standard Taguchi L8(27) array design was conducted with four single factors, water (W), nitrogen (N), phosphorus (P) and fragment size (C), and three interactions (N × P, N × W and P × W). Each of the four factors had two levels (1 = low level and 2 = high level). This study found that water was the most important contributor influencing S. breviflora root growth, followed by N and P, respectively. W2 and P2 additions both promoted root growth, whereas N2 addition significantly inhibited root growth. Though C2 had higher values of total root length, surface area, volume, number of tips and biomass than C1, its root growth rate was lower than C1, and its small size fragment had a higher capacity of root growth under low N addition. These findings suggest that clonal fragmentation may enhance the adaptation of S. breviflora in low nitrogen habitats, and that nitrogen is one of the limiting factors influencing their growth and distribution.
Evaluation of Carbonic Maceration Effect as a Pre-Treatment on the Drying Process of Strawberry
Authors: Ozcelik Muhammed Mustafa , Ozkan Gulcan , Karacabey Erkan
Published: December 2022

In the last decade, organic-based food materials have taken an increasing marketing share due to consumers’ interests. Strawberry is one of the world’s most important berry crops, with growing production. This study aimed to evaluate the drying process of organic strawberries and to determine the influences of process conditions on drying characteristics. To improve drying performance, carbonic maceration (CM) was investigated as a pre-treatment. The experimental design and the optimization of the drying with pre-treatment conditions were performed using statistical modeling (response surface method (RSM), central composite design (CCD)). Using the RSM, dependent variables such as drying time, total phenolic compound, antioxidant capacity, ascorbic acid concentration, and hue value were optimized as a function of operating conditions of CM pre-treatment and following the drying process. The results indicated that drying performance varied depending on drying temperature as well as process conditions of CM. Drying time was reduced by more than 30% with CM treatment compared to control. Furthermore, ascorbic acid content, antioxidant activity, and surface color of dried samples were protected better in the case of CM-pre-treated strawberries. Results showed that the optimum drying temperature, maceration pressure, maceration temperature, and maceration time parameters were 64.8 °C, 3.54 bar, 4.2 °C, and 4 h, respectively. The high potential of CM should be considered in terms of process improvement and product quality for drying processes. Thus, this study provides key outcomes in this respect. According to the obtained results, the CM was assessed as a promising technique applied before the drying of organic strawberries.
Effects of Cutting Stages and Additives on the Fermentation Quality of Triticale, Rye and Oat Silage in Qinghai-Tibet Plateau
Authors: Ma Jun , Dai Hanling , Liu Hancheng , Du Wenhua
Published: December 2022

The Qinghai–Tibet Plateau is sparsely populated and has vast grassland, which plays an important role in the development of animal husbandry. However, during the forage cutting season, frequent rainfall and low temperatures are consistently experienced, which makes it extremely difficult to produce quality hay. The best way to process hay is to produce silages. In this experiment, dry matter yield and silage fermentation quality of dominant annual forages, namely triticale, rye and oat, with Sila-Max and Sila-Mix lactic acid bacteria additives at the five cutting stages, i.e., heading, flowering, grouting, milky and dough stages, were determined. Triticale at the dough stage had the highest dry matter yield among the three forages at the five cutting stages. The optimum harvesting time for triticale, rye and oat to produce quality silage in the Qinghai–Tibet alpine area was the milky stage. Sila-Max lactic acid bacteria additives could significantly improve the fermentation qualities of triticale, rye and oat silages, but the fermentation effect of Sila-Mix on the three silages was not significant. The triticale variety ‘Gannong No.2’ is the best raw material to produce quality silages in the Qinghai–Tibet alpine area. Overall, quality silage could be made in the Qinghai–Tibet alpine area while using the triticale variety ‘Gannong No.2’ as the raw material, cutting it at the milky stage and adding Sila-Max as the lactic acid bacteria additive.
Research on a Map-Based Cooperative Navigation System for Spraying–Dosing Robot Group
Authors: Qin Jifeng , Wang Wang , Mao Wenju , Yuan Minxin , Liu Heng , Ren Zhigang , Shi Shuaiqi , Yang Fuzeng
Published: December 2022

To solve the problem encountered when the spraying robot has run out of medicine even though the spraying task on the field is not complete, we developed a spraying–dosing robot group and proposed a collaborative navigation system based on an orchard map. Firstly, we constructed a 3D orchard point cloud map and set up navigation path points on the projected map. Secondly, we developed a master–slave command-based cooperative navigation strategy, where the spraying robot was the master and the dosing robot was the slave. Finally, the spraying robot and the dosing robot completed the cooperative navigation on the constructed map by using the pure pursuit algorithm and D-A control algorithm, respectively. To validate the cooperative navigation system, we conducted field tests on the separate communication and navigation control. The results of communication experiments demonstrated that the packet loss rate was less than 5%, which satisfied communication requirements. The experimental results of the navigation control demonstrated that the maximum value of the absolute lateral error is 24.9 cm for the spraying robot and 29.7 cm for the dosing robot. The collaborative navigation system proposed in this research can meet the automatic navigation requirements of the spraying–dosing robot group for collaborative tasks in traditional orchards.
Evaluation and Identification of Stable Chickpea Lines for Yield-Contributing Traits from an Association Mapping Panel
Authors: Shimray Philanim Wungmarong , Bharadwaj C. , Patil B. S. , Sankar S. Mukesh , Kumar Neeraj , Reddy Sneha Priya Pappula , Singhal Tripti , Hegde Venkatraman , Parida Swarup K. , Roorkiwal Manish , Varshney Rajeev K. , Verma Preeti
Published: December 2022

An association mapping panel consisting of 380 genotypes of chickpea was evaluated for three different years, including 2014–2015, 2015–2016 and 2016–2017, for yield-contributing parameters, including the seed number and seed weight. The AMMI analysis presented mainly concentrated on the seed weight and seed number, which are the two most important yield-contributing traits. The genotypes contributed 93.08% of the total variance, while the interaction effect was comparatively low, with 4.1% for the two traits. AMMI biplot analysis identified IG5986, IG5982, ILC6025 and ICCV14307 as desirable genotypes for the seed weight and IG5893, ILC6891 and IG5856 for the seed number. Identifying stable genotypes would help in strategic planning for yield improvement through component trait breeding.