bluuebunny/crossref_metadata_2025 huggingface dataset:
Yin Huo Tang, a traditional Chinese herbal formula, relives ovariectomy and empty bottle stimulation-induced menopause-like symptoms in mice
Authors: Ye Yang , Zhang Bo , Li Yan , Xu Hong-Dan , Liu Xiu-Min , Huang Shu-Ming , Wang Rui , Li Dong
Published: October 2022

Background

Yin Huo Tang (YHT), a traditional Chinese herbal formula, is effectively used for the clinical treatment of menopause-like symptoms in China. This study aimed to investigate its efficacy on menopause-like symptoms in mice using behavioral tests and histopathological assessment, and to determine its possible mechanism of action based on network pharmacology.

Methods

Liquid chromatography-mass spectrometry (LC-MS) technology was used to identify the potential active ingredients of YHT. In mice, menopause-like symptoms were induced by combination of bilateral ovariectomy and empty bottle stimulation. The mice were then treated with the YHT aqueous extract for three weeks. Behavior, sleep state, body weight, organ index, and histomorphology were analyzed separately. Additionally, network pharmacology and molecular docking were used to predict the mechanisms underlying the action of YHT. Finally, serum estradiol was quantified to preliminarily verify the results of network pharmacology.

Results

YHT not only improved the behavior of mice (attack and explore behavior reduced; modify behavior increased) but also ameliorated the sleep state (sleep time increased and incubation time reduced). YHT reduced body weight, increased uterine weight, and improved the histomorphology of some organs. Network pharmacology and molecular docking analyses revealed that the estrogen signaling pathway might play a key role in attenuating menopause-like symptoms. Furthermore, YHT treatment reversed the reduction in serum estradiol levels.

Conclusions

YHT alleviates menopause-like symptoms in a mouse model, providing a rationale for using it as a potential therapeutic strategy.
Microbial biomarkers to identify areas of wetland sediments affected by massive fish farming
Authors: Lian Yanqing , Zhen Lisha , Fang Yan , Li Hao , Li Xiaona , Li Yang , Xu Xinhan
Published: October 2022

Massive fish farming for more than two decades, combined with external sources of pollution, deteriorated the quality of water and sediment and damaged the ecosystem in Honghu Lake. Although water quality has been improving, great challenges remain in restoring the healthy function of ecosystems for self-mitigation. With concerns about contaminants in the sediment that might be the source of pollution, water and sediment samples were collected systematically across the wetland. With the 16s rDNA gene sequence and multiple statistical analyses, this study was able to identify locations where sediments were mostly impacted by non-point source pollution from the surrounding agricultural areas and fish farming inside the lake. The clustering analysis identified two distinct clusters coincide with areas with and without fish farming. The LEfSe algorithm successfully distinguished the differentially abundant biomarkers between the two clusters. By combining the GIS techniques with microbial analysis, hotspots with hazardous pathogenic bacteria such as Rokubacteria, Elusimicrobia, and Fusobacteria in sediments were located. This study showed that comprehensive biodiversity analysis combined with spatial analysis could make the microbial information serve well as biomarkers and indicators for environmental and ecosystem restoration.
Environmental degradation in terms of health expenditure, education and economic growth. Evidence of novel approach
Authors: Bu Junqin , Ali Kishwar
Published: October 2022

Physical education benefits health and the environment because the world takes long-term steps to stop environmental degradation and its effects. Therefore, the present study examined the impact of health expenditure, education, economic growth, and population on environmental degradation in seven emerging economies from 2000 to 2019. The cross-sectional dependency (CSD) reflected the panel nations’ CSD, whereas the second-generation panel unit root test confirmed all indicators’ stationarity at first difference. Thus, the second-generation cointegration approach identified a long-term equation among the CO2, health expenditure, education, economic growth and population. The long-run empirical estimations derived from the PFMLOS and PDOLS method emphasized that education increases the region’s environmental sustainability and decreases CO2 emissions. Conversely, health expenditure, economic growth, and population increase CO2 emissions and reduce environmental quality in the E-7 bloc. Moreover, our findings are resilient to alternative measures by AMG and CCEMG, which might help policymakers develop long- and short-term initiatives for environmental protection. The study suggests adopting physical education and physical health activities to curb environmental degradation in the panel region.
Adsorption of Phosphate by Surface Precipitation on Lanthanum Carbonate Through In Situ Anion Substitution Reactions
Authors: Zhang Shaoyang , Xie Fazhi , Tang Zhi , Zhao Tianhui , Fang Mengyuan , Giesy John P.
Published: October 2022

Graphical Abstract
Development of bamboo- (Bambusa bambos) based bio-fence to protect field crops: Insights from a study in India's Bundelkhand region
Authors: Kumar Dinesh , Yadav Ram Swaroop , Kadam Darshan Manikrao , Ahirwar Lakhan Lal , Dohare Anil Kumar , Singh Gautam
Published: October 2022

Stray/wild animals can cause serious damages to crops, leading to accusations and counter accusations among villagers. In the Bundelkhand region of India, this problem is more severe due to “Anna Pratha,” that is, letting loose animals to open graze. Protective measures employed by farmers (barbed wire fencing, conventional fencing, etc.) are not fully effective and also require periodical maintenance, which involves additional costs and manpower. This necessitates the evolution of a cost-effective and long-term solution to minimize the problem. The current study seeks to evaluate the potential of thorny bamboo (Bambusa bambos) as a bio-fence creating deterrence to stray/wild animals at the ICAR—IISWC RC, the research farm of Datia, Madhya Pradesh, India. After 21 months of planting, bamboo plants attained the maximum plant height up to 4.47 m with the highest clump spread diameter of 30.50 cm. However, the growth of the bamboo bio-fence to be effective depends on the edaphic and management conditions. The findings revealed that planting bamboo at a distance of 80.00 cm in the continuous trench can be an effective bio-fence to avoid man–animal conflict. Huge crop losses were reported before the establishment of a bio-fence. In 2021–2022 (when bamboo plants turned into a closely spaced thicket, making a bio-fence), only two incidents of the invasion of animals were reported with no crop damage. The initial cost to develop a bamboo bio-fence was estimated at INR 5,796 for a length of 100 m. Therefore, the present study suggests that bamboo bio-fencing is an economical and effective crop protection measure against damage by wild/stray animals.
Managing nature-based solutions in fire-prone ecosystems: Competing management objectives in California forests evaluated at a landscape scale
Authors: Herbert Claudia , Haya Barbara K. , Stephens Scott L. , Butsic Van
Published: October 2022

California’s cap-and-trade compliance offset market incentivizes forest managers to maintain elevated carbon stocks. It provides these incentives without enforcing standardized fire mitigation practices despite many projects being located in fire prone regions. Here, we evaluated the difference between management actions in California forests that participated in the carbon offset market versus those that engaged with state programs to reduce wildfire risk via fuel reduction treatments. Using remotely sensed data from the California Forest Observatory and the Moderate Resolution Imaging Spectroradiometer, we compared the vertical forest structure and vegetation canopy trends on forest offsets with forests that are receiving fuel treatment. We found California forests managed for carbon under the Improved Forest Management (IFM) program by the California Air Resources Board had higher levels of biomass than forests managed for fire risk reduction as indicated by 2016 lidar-estimated fuel loads. In addition, IFM-participating forests did not reduce their fuel loads between 2016 and 2020, whereas lands receiving grants for fuel management did, indicating that on average, the IFM projects were not engaging in fuel reduction efforts. However, despite the differences in fuel management between IFM projects and active fuel treatments, we found that both types of management saw a declining trend in vegetation greenness between 2015 and 2021. While declining greenness is expected of active fuel treatments associated with vegetation removal, such a trend in the case of IFM indicates additional wildfire risk. Managing forests for long-term carbon storage and sequestration requires consideration of fire risk mitigation. Given the little evidence of fuel reduction in the first decade of IFM projects implementation we question whether the century-long duration of carbon stocks in these offsets is realistic. We recommend that policymakers reevaluate the incentives directed at carbon stock preservation or expansion to better encompass the growing wildfire risk in California.
Single-cell sequencing technologies in bladder cancer research: Applications and challenges
Authors: Lyu Tianqi , Lin Yuanbin , Wu Kerong , Cao Zhanglei , Zhang Qian , Zheng Jianping
Published: October 2022

Bladder cancer is among the most common malignant tumors with highly heterogeneous molecular characteristics. Despite advancements of the available therapeutic options, several bladder cancer patients exhibit unsatisfactory clinical outcomes. The lack of specific biomarkers for effective targeted therapy or immunotherapy remains a major obstacle in treating bladder cancer. The rapid development of single-cell techniques is transforming our understanding of the intra-tumoral heterogeneity, thereby providing us with a powerful high-throughput sequencing tool that can reveal tumorigenesis, progression, and invasion in bladder tumors. In this review, we summarise and discuss how single-cell sequencing technologies have been applied in bladder cancer research, to advance our collective knowledge on the heterogeneity of bladder tumor cells, as well as to provide new insights into the complex ecosystem of the tumor microenvironment. The application of single-cell approaches also uncovers the therapeutic resistance mechanism in bladder cancer and facilitates the detection of urinary-exfoliated tumor cells. Moreover, benefiting from the powerful technical advantages of single-cell techniques, several key therapeutic targets and prognostic models of bladder cancer have been identified. It is hoped that this paper can provide novel insights into the precision medicine of bladder cancer.
Transcriptional data analysis reveals the association between infantile hemangiomas and venous malformations
Authors: Huang Biao , Zhang Ping , Zhong Yuan-Yuan , Wang Kuan , Chen Xiao-Ming , Yu Dao-Jiang
Published: October 2022

Background: Infantile hemangiomas (IH) and venous malformations (VM) are the most common types of vascular abnormalities that seriously affect the health of children. Although there is evidence that these two diseases share some common genetic changes, the underlying mechanisms need to be further studied.

Methods: The microarray datasets of IH (GSE127487) and VM (GSE7190) were downloaded from GEO database. Extensive bioinformatics methods were used to investigate the common differentially expressed genes (DEGs) of IH and VM, and to estimate their Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Trough the constructing of protein-protein interaction (PPI) network, gene models and hub genes were obtained by using Cytoscape and STRING. Finally, we analyzed the co-expression and the TF-mRNA-microRNA regulatory network of hub genes.

Results: A total of 144 common DEGs were identified between IH and VM. Functional analysis indicated their important role in cell growth, regulation of vasculature development and regulation of angiogenesis. Five hub genes (CTNNB1, IL6, CD34, IGF2, MAPK11) and two microRNA (has-miR-141-3p, has-miR-150-5p) were significantly differentially expressed between IH and normal control (p < 0.05).

Conclusion: In conclusion, our study investigated the common DEGs and molecular mechanism in IH and VM. Identified hub genes and signaling pathways can regulate both diseases simultaneously. This study provides insight into the crosstalk of IH and VM and obtains several biomarkers relevant to the diagnosis and pathophysiology of vascular abnormalities.
Complement-targeting therapeutics for ischemia-reperfusion injury in transplantation and the potential for ex vivo delivery
Authors: Delaura Isabel F. , Gao Qimeng , Anwar Imran J. , Abraham Nader , Kahan Riley , Hartwig Matthew G. , Barbas Andrew S.
Published: October 2022

Organ shortages and an expanding waitlist have led to increased utilization of marginal organs. All donor organs are subject to varying degrees of IRI during the transplant process. Extended criteria organs, including those from older donors and organs donated after circulatory death are especially vulnerable to ischemia-reperfusion injury (IRI). Involvement of the complement cascade in mediating IRI has been studied extensively. Complement plays a vital role in the propagation of IRI and subsequent recruitment of the adaptive immune elements. Complement inhibition at various points of the pathway has been shown to mitigate IRI and minimize future immune-mediated injury in preclinical models. The recent introduction ofex vivomachine perfusion platforms provides an ideal window for therapeutic interventions. Here we review the role of complement in IRI by organ system and highlight potential therapeutic targets for intervention duringex vivomachine preservation of donor organs.
Pre-existing humoral immunity to low pathogenic human coronaviruses exhibits limited cross-reactive antibodies response against SARS-CoV-2 in children
Authors: Li Nina , Li XueYun , Wu Jiani , Zhang Shengze , Zhu Lin , Chen Qiqi , Fan Ying , Wu Zhengyu , Xie Sidian , Chen Qi , Wang Ning , Wu Nan , Luo Chuming , Shu Yuelong , Luo Huanle
Published: October 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes asymptomatic or mild symptoms, even rare hospitalization in children. A major concern is whether the pre-existing antibodies induced by low pathogenic human coronaviruses (LPH-CoVs) in children can cross-react with SARS-CoV-2. To address this unresolved question, we analyzed the pre-existing spike (S)-specific immunoglobin (Ig) G antibodies against LPH-CoVs and the cross-reactive antibodies against SARS-CoV-2 in 658 serum samples collected from children prior to SARS-CoV-2 outbreak. We found that the seroprevalence of these four LPH-CoVs reached 75.84%, and about 24.64% of the seropositive samples had cross-reactive IgG antibodies against the nucleocapsid, S, and receptor binding domain antigens of SARS-CoV-2. Additionally, the re-infections with different LPH-CoVs occurred frequently in children and tended to increase the cross-reactive antibodies against SARS-CoV-2. From the forty-nine serum samples with cross-reactive anti-S IgG antibodies against SARS-CoV-2, we found that seven samples with a median age of 1.4 years old had detected neutralizing activity for the wild-type or mutant SARS-CoV-2 S pseudotypes. Interestingly, all of the seven samples contained anti-S IgG antibodies against HCoV-OC43. Together, these data suggest that children’s pre-existing antibodies to LPH-CoVs have limited cross-reactive neutralizing antibodies against SRAS-CoV-2.
Characteristics of clinical trials for non-small cell lung cancer therapeutic vaccines registered on ClinicalTrials.gov
Authors: Gu Wenyue , Xu Yangjie , Chen Xiaohong , Jiang Hao
Published: October 2022

Background

Even after complete surgical treatment or chemotherapy, Non-Small Cell Lung Cancer (NSCLC) patients are also at substantial risk for recurrence and spread trend. Therapeutic cancer vaccination could increase the anti-tumor immune response and prevent tumor relapse. This study aimed to assess the characteristics of NSCLC therapeutic vaccines registered on ClinicalTrials.gov.

Methods

We conducted a cross-sectional, descriptive study of clinical trials for Non-Small Cell Lung Cancer Therapeutic Vaccines Registered on ClinicalTrials.gov (https://clinicaltrials.gov/) through March 17, 2022.

Results

This study encompassed 117 registered trials included for data analysis. The number of trials was significantly correlated with a beginning year (r = 0.504, P < 0.010). Of these trials, 45.30% were completed, 12.82% were terminated, and 8.55% were withdrawn. More than half of trials (52.99%) were funded by industry, and more than half of trials (52.14%) were located in economically developed North America. Regarding study designs of these trials, 27.35% were randomized, 52.14% were single group assignment, 83.76% were without masking, 35.90% were phase 1, and more than half of the trials (56.41%) recruited less than 50 participants. The highest proportion of vaccine types was protein/peptide vaccines (41.88%). Regarding TNM staging, the highest proportion of the trials is stage III-IV (26.50%).

Conclusion

The number of clinical trials about the cancer therapeutic vaccines was sustained an increase in recent years. The main characteristic of clinical trials for NSCLC therapeutic vaccines is lack of randomized control, lack of mask, and recruiting less than 50 participants. In recent years, the protein/peptide vaccines for NSCLC active immunotherapy have been well studied.
Dietary phytate primes epithelial antibacterial immunity in the intestine
Authors: Hashimoto-Hill Seika , Colapietro Luisa , Woo Vivienne , Antonacci Simona , Whitt Jordan , Engleman Laura , Alenghat Theresa
Published: October 2022

Although diet has long been associated with susceptibility to infection, the dietary components that regulate host defense remain poorly understood. Here, we demonstrate that consuming rice bran decreases susceptibility to intestinal infection with Citrobacter rodentium, a murine pathogen that is similar to enteropathogenic E. coli infection in humans. Rice bran naturally contains high levels of the substance phytate. Interestingly, phytate supplementation also protected against intestinal infection, and enzymatic metabolism of phytate by commensal bacteria was necessary for phytate-induced host defense. Mechanistically, phytate consumption induced mammalian intestinal epithelial expression of STAT3-regulated antimicrobial pathways and increased phosphorylated STAT3, suggesting that dietary phytate promotes innate defense through epithelial STAT3 activation. Further, phytate regulation of epithelial STAT3 was mediated by the microbiota-sensitive enzyme histone deacetylase 3 (HDAC3). Collectively, these data demonstrate that metabolism of dietary phytate by microbiota decreases intestinal infection and suggests that consuming bran and other phytate-enriched foods may represent an effective dietary strategy for priming host immunity.
Autoantibodies - enemies, and/or potential allies?
Authors: Ma Hui , Murphy Caroline , Loscher Christine E. , O’Kennedy Richard
Published: October 2022

Autoantibodies are well known as potentially highly harmful antibodies which attack the host via binding to self-antigens, thus causing severe associated diseases and symptoms (e.g. autoimmune diseases). However, detection of autoantibodies to a range of disease-associated antigens has enabled their successful usage as important tools in disease diagnosis, prognosis and treatment. There are several advantages of using such autoantibodies. These include the capacity to measure their presence very early in disease development, their stability, which is often much better than their related antigen, and the capacity to use an array of such autoantibodies for enhanced diagnostics and to better predict prognosis. They may also possess capacity for utilization in therapy, in vivo. In this review both the positive and negative aspects of autoantibodies are critically assessed, including their role in autoimmune diseases, cancers and the global pandemic caused by COVID-19. Important issues related to their detection are also highlighted.
Comparison of the macrofilaricidal efficacy of oxfendazole and its isomers against the rodent filaria Litomosoides sigmodontis
Authors: Risch Frederic , Koschel Marianne , Lenz Benjamin , Specht Sabine , Hoerauf Achim , Hübner Marc P. , Scandale Ivan
Published: October 2022

Oxfendazole is one of the lead macrofilaricidal candidates for the treatment of onchocerciasis and lymphatic filariasis. Originally, oxfendazole was developed for the veterinary market, where it is mainly used to treat intestinal helminth infections. In humans, oxfendazole was proven to be safe in multiple ascending dose studies. Furthermore, previous experimental studies demonstrated that the benzimidazoles class is active in animals and humans against filarial nematodes. In the present study, we have compared the efficacy of oxfendazole isomers with the commercially available racemic mixture Dolthene against the rodent filaria Litomosoides sigmodontis in female BALB/c mice. Treatment with either the isomers or Dolthene led to a reduction of the adult worm burden by 94-98% following the ten-day treatment and by 72% (oxfendazole (-)), 85% (oxfendazole (+)) and 91% (Dolthene) following the five-day treatment. No statistically significant differences in the macrofilaricidal efficacy against L. sigmodontis were observed for both isomers and Dolthene. Metabolites of oxfendazole are fenbendazole and fenbendazole sulfone. Two hours after treatment with Dolthene and both oxfendazole isomers, fenbendazole sulfone, but rarely fenbendazole, was detected. The oxfendazole (-) isomer was metabolised at the highest rate to fenbendazole sulfone. Furthermore, oxfendazole isomers have a comparable pharmacokinetic profile in dogs. In conclusion, our data does not point at the development of a single isomer for future use in humans.
Fishermen’s perceptions of constraints on adaptive capacity in the California market squid and California spiny lobster fisheries
Authors: Powell Farrah , Levine Arielle , Ordonez-Gauger Lucia
Published: October 2022

Planning for resiliency in the face of unprecedented environmental change requires understanding the factors that constrain fishermen’s capacity to adapt. Scholars have highlighted numerous factors that may facilitate or constrain fishermen’s adaptive capacity, but ultimately fishermen act based on their own perceptions of their ability to respond and adapt to change within their broader social, environmental, and governance context. Subjective assessments of adaptive capacity are limited, yet critical, given that in the face of stressors, people’s actions are facilitated or constrained by their subjective perceptions of their capacity to take action. Using fishermen interviews and feedback sessions, we explored fishermen’s perceptions of constraints on their ability to adapt to change in two fisheries in the California Current System: California spiny lobster and California market squid. Our comparative assessment revealed important similarities and differences with regard to the likelihood that fishermen would perceive a given factor as a constraint, as well as the extent to which different domains of adaptive capacity, including diversity and flexibility in livelihood options, knowledge, and access to physical and financial capital, influence fishermen’s perceptions of constraints. Constraints relating to fishery governance, including permit access, fishery regulations, and broader concerns with fishery management were the most commonly perceived constraints in both fisheries. Individual-level constraints including mobility and knowledge of other fisheries and fishing locations were less frequently cited and significantly more likely to be perceived as constraints by spiny lobster fishermen than market squid fishermen. Our results highlight the importance of considering interactions between factors constraining different elements of adaptive capacity given that the broader governance context of fisheries can inhibit individual-level adaptive strategies. Overcoming barriers to adaptation necessitates planned and participatory governance processes that strengthen fishermen’s individual agency and ability to take meaningful action in the face of change.
Probabilistic extreme SST and marine heatwave forecasts in Chesapeake Bay: A forecast model, skill assessment, and potential value
Authors: Ross Andrew C. , Stock Charles A.
Published: October 2022

We test whether skillful 35-day probabilistic forecasts of estuarine sea surface temperature (SST) are possible and whether these forecasts could potentially be used to reduce the economic damages associated with extreme SST events. Using an ensemble of 35-day retrospective forecasts of atmospheric temperature and a simple model that predicts daily mean SST from past SST and forecast atmospheric temperature, we create an equivalent ensemble of retrospective SST forecasts. We compare these SST forecasts with reference forecasts of climatology and damped persistence and find that the SST forecasts are skillful for up to two weeks in the summer. Then, we post-process the forecasts using nonhomogeneous Gaussian regression and assess whether the resulting calibrated probabilistic forecasts are more accurate than the probability implied by the raw model ensemble. Finally, we use an idealized framework to assess whether these probabilistic forecasts can valuably inform decisions to take protective action to mitigate the effects of extreme temperatures and heatwaves. We find that the probabilistic forecasts provide value relative to a naive climatological forecast for 1-2 weeks of lead time, and the value is particularly high in cases where the cost of protection is small relative to the preventable losses suffered when a heatwave occurs. In most cases, the calibrated probabilistic forecasts are also more valuable than deterministic forecasts based on the ensemble mean and naive probabilistic forecasts based on damped persistence. Probabilistic SST forecasts could provide substantial value if applied to adaptively manage the rapid impacts of extreme SSTs, including managing the risks of catch-and-release mortality in fish and Vibrio bacteria in oysters.
Ultrasonic inspection of near surface defects with additive manufactured metasurface lens
Authors: Zhu Qi , Zhang Yuanjun , Zhang Haiyan , Memoli Gianluca
Published: October 2022

Acoustic metasurfaces have been increasingly explored in the past 10 years because of their compact structure and wave manipulation capabilities. In this work, we explore the feasibility of using an acoustic metasurface lens (MSL) for the characterization of near surface defects in NDT. First, we delimited the design space for a MSL operating at 100 kHz, in terms of spatial footprint and materials, and then realized a library of labyrinthine cells optimized for transmission. Then, we assembled the unit cells into lens and simulated its performance in a hole inspection process. The time-domain simulations predicted an increase in scattering and interface effects but, thanks to the focusing behavior, they also showed a much lower drop in the peak amplitude (20%, compared to 70% without MSL). Finally, we validated the simulations using a UTR9000-based MSL on an acrylic sample. Experiments with the MSL showed a converging energy profile, reduced to half of its width without the lens, and the presence of additional transmission peaks allowed to determine the defect diameter with excellent precision, thus circumventing near surface interference. Our study may be the first step towards flexible beam control systems based on a single transducer.
Establishment of a model for predicting delayed post-polypectomy bleeding: A real-world retrospective study
Authors: Lu Yu , Zhou Xiaoying , Chen Han , Ding Chao , Si Xinmin
Published: October 2022

Background

Delayed post-polypectomy bleeding (DPPB) is the most common complication which occurs within 30 days after polypectomy, it has become rather common with the widespread of colorectal cancer screening. It is important to clarified predictors of DPPB and identify patients at high risk.

Materials and methods

This was a real-world retrospective study based on medical records from The First Affiliated Hospital of Nanjing Medical University. Cases of patients who underwent colonoscopic polypectomy between January 2016 and December 2020 were reviewed to identify risk factors of DPPB. We use the LASSO-Logistic regression analysis model to identify independent predictors and create a predictive model. The model finally got visualized by developing a nomogram.

Results

Colonoscopic polypectomy was done on 16,925 patients in our study. DPPB occurred in 125 (0.74%) of these instances. In multivariate analysis, age, sex, hypertension, polyp location, polyp size, and operative modality were found to be independent risk factors and were integrated for the construction of a nomogram. The model’s C-index is 0.801 (95%CI: 0.761–0.846). We also found polyps located at the right semicolon and polyp ≥ 1 cm associated with active bleeding under the therapeutic colonoscopy.

Conclusion

Young age, male, hypertension, polyp ≥ 1 cm, proximal colon location and operative modality were finally identified as significant predictors of DPPB. We developed and validated a nomogram which performs well in predicting the incidence of DPPB, the model we established can be used as a valuable screening tool to identify patients who are at high risk of bleeding.
Persistence of plasmid and tet(X4) in an Escherichia coli isolate coharboring blaNDM-5 and mcr-1 after acquiring an IncFII tet(X4)-positive plasmid
Authors: Xiao Xia , Liu Ziyi , Chen Xiaojun , Peng Kai , Li Ruichao , Liu Yuan , Wang Zhiqiang
Published: October 2022

The prevalence of plasmid-mediated tigecycline resistance gene tet(X4) is presenting an increasing trend. Once tet(X4)-bearing plasmids are captured by multidrug-resistant bacteria, such as blaNDM and mcr-coharboring bacteria, it will promote bacteria to develop an ultra-broad resistance spectrum, limiting clinical treatment options. However, little is known about the destiny of such bacteria or how they will evolve in the future. Herein, we constructed a multidrug-resistant bacteria coharboring tet(X4), blaNDM-5, and mcr-1 by introducing a tet(X4)-bearing plasmid into a blaNDM-5 and mcr-1 positive E. coli strain. Subsequently, the stability of tet(X4) and the plasmid was measured after being evolved under tigecycline or antibiotic-free circumstance. Interestingly, we observed both tet(X4)-bearing plasmids in tigecycline treated strains and non-tigecycline treated strains were stable, which might be jointly affected by the increased conjugation frequency and the structural alterations of the tet(X4)-positive plasmid. However, the stability of tet(X4) gene showed different scenarios in the two types of evolved strains. The tet(X4) gene in non-tigecycline treated strains was stable whereas the tet(X4) gene was discarded rapidly in tigecycline treated strains. Accordingly, we found the expression levels of tet(X4) gene in tigecycline-treated strains were several times higher than in non-tigecycline treated strains and ancestral strains, which might in turn impose a stronger burden on the host bacteria. SNPs analysis revealed that a myriad of mutations occurred in genes involving in conjugation transfer, and the missense mutation of marR gene in chromosome of tigecycline treated strains might account for the completely different stability of tet(X4)-bearing plasmid and tet(X4) gene. Collectively, these findings shed a light on the possibility of the emergence of multidrug resistant bacteria due to the transmission of tet(X4)-bearing plasmid, and highlighted that the antibiotic residues may be critical to the development of such bacteria.
Dietary rosemary extract modulated gut microbiota and influenced the growth, meat quality, serum biochemistry, antioxidant, and immune capacities of broilers
Authors: Liu Yang , Li Chuang , Huang Xuan , Zhang Xu , Deng Ping , Jiang Guitao , Dai Qiuzhong
Published: October 2022

After the legislative ban on the utilization of antibiotics in animal feed, phytochemical substances gained increasing attention as alternatives to antibiotics because of their bioactivities and safety for animals. The present study aimed to investigate the influence of dietary rosemary extract (RE) on growth performance, meat quality, serum biochemistry, antioxidant and immune capacities, and gut microbiota composition of broilers. By exploring connections among RE, physiological characteristics of broilers, and key microbiota, we sought to provide evidence for the utilization of RE in poultry feed. A total of 280 1-d-old female AA broilers were randomly separated into five groups, and were fed a basal diet supplemented with 0, 250, 500, 750, and 1,000 mg/kg of RE, respectively. Results showed that with regard to growth performance, both 500 and 750 mg/kg RE reduced the broiler feed-to-gain ratio from 1 to 21 d (P = 0.018). Regarding meat quality, all compositions of dietary RE reduced cooking loss of breast muscle (P < 0.01), and 500 and 1,000 mg/kg RE reduced the cooking loss of thigh muscle (P = 0.045). Regarding serum biochemical indexes, 500 mg/kg RE reduced ALB, TCHO, HDL-C, and LDL-C, and 750 mg/kg RE reduced GLU, TP, ALB, UA, TG, TCHO, HDL-C, and LDL-C (P < 0.01). Regarding antioxidant and immune capacities, 250, 500, 750, and 1,000 mg/kg RE increased T-AOC, GSH-Px, SOD, CAT, IL-2, IgA, IgG, and IgM levels (P < 0.01), and decreased serum MDA level (P < 0.01). RE at 750 mg/kg showed similar effects on growth performance, meat quality, and antioxidative and immune capacities, but a better influence on serum biochemical indexes of broilers compared with 500 mg/kg. Further analysis was conducted to investigate the effect of 750 mg/kg dietary RE on the gut microbial composition of broilers, and the results showed that 750 mg/kg RE reduced the relative abundance of g_Lachnoclostridium, g_Escherichia_Shigella, and g_Marvinbryantia (P <0.05, LDA score >2), which were negatively correlated to antioxidative and immune-associated parameters (P < 0.05). In conclusion, 750 mg/kg dietary RE was shown to have certain beneficial effects on growth performance and meat quality, and hypolipidemic and hypoglycemic effects on broilers. Furthermore, dietary RE improved antioxidant and immune capacities, which was partially attributed to the reduced abundance of certain pathogenic bacteria in broilers.
Dissemination of blaNDM-5 and mcr-8.1 in carbapenem-resistant Klebsiella pneumoniae and Klebsiella quasipneumoniae in an animal breeding area in Eastern China
Authors: Yang Chengxia , Han Jingyi , Berglund Björn , Zou Huiyun , Gu Congcong , Zhao Ling , Meng Chen , Zhang Hui , Ma Xianjun , Li Xuewen
Published: October 2022

Animal farms have become one of the most important reservoirs of carbapenem-resistant Klebsiella spp. (CRK) owing to the wide usage of veterinary antibiotics. “One Health”-studies observing animals, the environment, and humans are necessary to understand the dissemination of CRK in animal breeding areas. Based on the concept of “One-Health,” 263 samples of animal feces, wastewater, well water, and human feces from 60 livestock and poultry farms in Shandong province, China were screened for CRK. Five carbapenem-resistant Klebsiella pneumoniae (CRKP) and three carbapenem-resistant Klebsiella quasipneumoniae (CRKQ) strains were isolated from animal feces, human feces, and well water. The eight strains were characterized by antimicrobial susceptibility testing, plasmid conjugation assays, whole-genome sequencing, and bioinformatics analysis. All strains carried the carbapenemase-encoding gene blaNDM-5, which was flanked by the same core genetic structure (IS5-blaNDM-5-bleMBL-trpF-dsbD-IS26-ISKox3) and was located on highly related conjugative IncX3 plasmids. The colistin resistance gene mcr-8.1 was carried by three CRKP and located on self-transmissible IncFII(K)/IncFIA(HI1) and IncFII(pKP91)/IncFIA(HI1) plasmids. The genetic context of mcr-8.1 consisted of IS903-orf-mcr-8.1-copR-baeS-dgkA-orf-IS903 in three strains. Single nucleotide polymorphism (SNP) analysis confirmed the clonal spread of CRKP carrying-blaNDM-5 and mcr-8.1 between two human workers in the same chicken farm. Additionally, the SNP analysis showed clonal expansion of CRKP and CRKQ strains from well water in different farms, and the clonal CRKP was clonally related to isolates from animal farms and a wastewater treatment plant collected in other studies in the same province. These findings suggest that CRKP and CRKQ are capable of disseminating via horizontal gene transfer and clonal expansion and may pose a significant threat to public health unless preventative measures are taken.
Degradation potential of alkanes by diverse oil-degrading bacteria from deep-sea sediments of Haima cold seep areas, South China Sea
Authors: Lyu Lina , Li Jie , Chen Yu , Mai Zhimao , Wang Lin , Li Qiqi , Zhang Si
Published: October 2022

Marine oil spills are a significant concern worldwide, destroying the ecological environment and threatening the survival of marine life. Various oil-degrading bacteria have been widely reported in marine environments in response to marine oil pollution. However, little information is known about culturable oil-degrading bacteria in cold seep of the deep-sea environments, which are rich in hydrocarbons. This study enriched five oil-degrading consortia from sediments collected from the Haima cold seep areas of the South China Sea. Parvibaculum, Erythrobacter, Acinetobacter, Alcanivorax, Pseudomonas, Marinobacter, Halomonas, and Idiomarina were the dominant genera. Further results of bacterial growth and degradation ability tests indicated seven efficient alkane-degrading bacteria belonging to Acinetobacter, Alcanivorax, Kangiella, Limimaricola, Marinobacter, Flavobacterium, and Paracoccus, whose degradation rates were higher in crude oil (70.3–78.0%) than that in diesel oil (62.7–66.3%). From the view of carbon chain length, alkane degradation rates were medium chains > long chains > short chains. In addition, Kangiella aquimarina F7, Acinetobacter venetianus F1, Limimaricola variabilis F8, Marinobacter nauticus J5, Flavobacterium sediminis N3, and Paracoccus sediminilitoris N6 were first identified as oil-degrading bacteria from deep-sea environments. This study will provide insight into the bacterial community structures and oil-degrading bacterial diversity in the Haima cold seep areas, South China Sea, and offer bacterial resources to oil bioremediation applications.
Population analysis of heavy metal and biocide resistance genes in Salmonella enterica from human clinical cases in New Hampshire, United States
Authors: Souza Stephanie S. R. , Turcotte Madison R. , Li Jinfeng , Zhang Xinglu , Wolfe Kristin L. , Gao Fengxiang , Benton Christopher S. , Andam Cheryl P.
Published: October 2022

Microbes frequently encounter heavy metals and other toxic compounds generated from natural biogeochemical processes and anthropogenic activities. Here, we analyzed the prevalence and association of genes conferring resistance to heavy metals, biocides, and antimicrobial compounds in 394 genome sequences of clinical human-derived S. enterica from New Hampshire, USA. The most prevalent was the gold operon (gesABC-golTSB), which was present in 99.2% of the genomes. In contrast, the other five heavy metal operons (arsenic, copper, mercury, silver, tellurite) were present in 0.76% (3/394)–5.58% (22/394) of the total population. The heavy metal operons and three biocide resistance genes were differentially distributed across 15 sequence types (STs) and 16 serotypes. The number of heavy metal operons and biocide resistance genes per genome was significantly associated with high number of antimicrobial resistance (AMR) genes per genome. Notable is the mercury operon which exhibited significant association with genes conferring resistance to aminoglycosides, cephalosporins, diaminopyrimidine, sulfonamide, and fosfomycin. The mercury operon was co-located with the AMR genes aac(3)-IV, ant(3”)-IIa, aph(3’)-Ia, and aph(4)-Ia, CTX-M-65, dfrA14, sul1, and fosA3 genes within the same plasmid types. Lastly, we found evidence for negative selection of individual genes of each heavy metal operon and the biocide resistance genes (dN/dS < 1). Our study highlights the need for continued surveillance of S. enterica serotypes that carry those genes that confer resistance to heavy metals and biocides that are often associated with mobile AMR genes. The selective pressures imposed by heavy metals and biocides on S. enterica may contribute to the co-selection and spread of AMR in human infections.
ADAMTS6: Emerging roles in cardiovascular, musculoskeletal and cancer biology
Authors: Mead Timothy J.
Published: October 2022

ADAMTS family members control mammalian development and disease, primarily through their function as proteases, by regulation of extracellular matrix composition. Until recently, ADAMTS6 was known as one of the orphan proteinases of the nineteen-member family with a relatively unknown expression pattern and function. Emerging focus on this enzyme has started to uncover these unknowns and revealed a vast importance and requirement of ADAMTS6 in cardiovascular and musculoskeletal development. In addition, ADAMTS6 has been linked to numerous disease settings including several types of cancer. This review summarizes the necessity of ADAMTS6 during development, its role in disease and requirement for essential prospective studies to fully realize its biological implications and potential for therapeutic intervention.
Exploring therapeutic potential of mitophagy modulators using Drosophila models of Parkinson’s disease
Authors: Asthana Jyotsna , Shravage Bhupendra V.
Published: October 2022

Parkinson’s disease (PD) is the second most popular age-associated neurodegenerative disorder after Alzheimer’s disease. The degeneration of dopaminergic neurons, aggregation of α-synuclein (α-syn), and locomotor defects are the main characteristic features of PD. The main cause of a familial form of PD is associated with a mutation in genes such as SNCA, PINK1, Parkin, DJ-1, LRKK2, and others. Recent advances have uncovered the different underlying mechanisms of PD but the treatment of PD is still unknown due to the unavailability of effective therapies and preventive medicines in the current scenario. The pathophysiology and genetics of PD have been strongly associated with mitochondria in disease etiology. Several studies have investigated a complex molecular mechanism governing the identification and clearance of dysfunctional mitochondria from the cell, a mitochondrial quality control mechanism called mitophagy. Reduced mitophagy and mitochondrial impairment are found in both sporadic and familial PD. Pharmacologically modulating mitophagy and accelerating the removal of defective mitochondria are of common interest in developing a therapy for PD. However, despite the extensive understanding of the mitochondrial quality control pathway and its underlying mechanism, the therapeutic potential of targeting mitophagy modulation and its role in PD remains to be explored. Thus, targeting mitophagy using chemical agents and naturally occurring phytochemicals could be an emerging therapeutic strategy in PD prevention and treatment. We discuss the current research on understanding the role of mitophagy modulators in PD using Drosophila melanogaster as a model. We further explore the contribution of Drosophila in the pathophysiology of PD, and discuss comprehensive genetic analysis in flies and pharmacological drug screening to develop potential therapeutic molecules for PD.
Neurochemical atlas of the cat spinal cord
Authors: Veshchitskii Aleksandr , Shkorbatova Polina , Merkulyeva Natalia
Published: October 2022

The spinal cord is a complex heterogeneous structure, which provides multiple vital functions. The precise surgical access to the spinal regions of interest requires precise schemes for the spinal cord structure and the spatial relation between the spinal cord and the vertebrae. One way to obtain such information is a combined anatomical and morphological spinal cord atlas. One of the widely used models for the investigation of spinal cord functions is a cat. We create a single cell-resolution spinal cord atlas of the cat using a variety of neurochemical markers [antibodies to NeuN, choline acetyltransferase, calbindin 28 kDa, calretinin, parvalbumin, and non-phosphorylated heavy-chain neurofilaments (SMI-32 antibody)] allowing to visualize several spinal neuronal populations. In parallel, we present a map of the spatial relation between the spinal cord and the vertebrae for the entire length of the spinal cord.
Is there an epilepsy belt of high prevalence rate in China?
Authors: Wu Li , Ye Zeming , Li Youfu , Luo Ming , Li Shaojun , Mi Hongyan , Qiu Zhihua , Jiang Yongjun
Published: October 2022

Background

Epilepsy is one of the leading neurological diseases. Our study is aimed to determine whether there is a focal region of high epilepsy prevalence in China.

Methods

All studies published between 1981 and 2020 investigating the prevalence of epilepsy in China were systematically reviewed. The geographical location, sample size, number of cases, urbanization rate, gross domestic product (GDP) per capita, percentage of <15 years old, and medical insurance per capita were derived and analyzed. Criteria for a provincial region of high prevalence was defined as with higher epilepsy prevalence than the average prevalence of epilepsy in China.

Results

A total of 60 studies provided data on the prevalence of epilepsy in 29 of 33 provincial regions of China. The average prevalence in China was 1.68 per 1,000, and 12 provincial regions met our criteria for a region of high epilepsy prevalence and constitute an epilepsy belt ranging along the division between the second step and the third step of China. The prevalence in the epilepsy belt was 331.9 per 100,000 population compared with 125.3 per 100,000 in regions outside the belt (P < 0.05). Surprisingly, there was no significant difference in sample size, number of cases, urbanization rate, GDP per capita, percentage of <15 years old, or medical insurance per capita between the regions in and outside the epilepsy belt.

Conclusions

An epilepsy belt of high prevalence exists in 12 provincial regions locating along the division between the second step and the third step of China.