bluuebunny/crossref_metadata_2025 huggingface dataset:
Biogenic catalysis in sulphide minerals' weathering processes and acid mine drainage genesis.
Authors: Kušnierová Mária , Praščáková Mária , Nowak Anna K , Gorazda Katarzyna , Wzorek Zbigniew
Published: February 2019

Bioleaching and biogenesis are the main outputs from a large group of environmental processes participating in the natural material cycle, used in raw materials processing. Bio-oxidation reactions are the main basis for bioleaching procedures, often participating in parallel leaching processes. During the leaching processes of polycomponent sulphide substrates, the factor of process selection also plays an important role, being in direct relation to the electric properties and galvanic effect occurring between the individual components of the leaching substrate. This work gives a summary of the results of a research focused on the possibilities of using biotechnological procedures for treatment of Slovak sulphide ores. The object of the research is extraction of valuable metals, undesirable admixtures and degradation of crystal lattice of sulphides for subsequent chemical leaching processing of precious metals. The results of experiments on the existence of biogenic processes in situ on waste dumps from exploitation containing residual sulphides are also presented. The processes result in acid mine drainage water generation. These waters are strongly mineralised (over 48 g/L) and of low pH; that is why they are very caustic. The arsenic content (2.558 mg/L) in outflowing waters from old mines is high and over the limits set by the law.
The correlation analysis of WWOX expression and cancer related genes in neuroblastoma- a real time RT-PCR study.
Authors: Nowakowska Magdalena , Płuciennik Elżbieta , Wujcicka Wioletta I , Sitkiewicz Anna , Kazanowska Bernarda , Zielińska Elżbieta , Bednarek Andrzej K
Published: February 2019

Neuroblastoma is one of the most common paediatric cancers, described as unpredictable due to diverse patterns of behaviour. WWOX is a tumour suppressor gene whose expression is reduced in many tumour types. Loss of its expression was shown to correlate with more aggressive disease stage and mortality rate. The aim of this study was to investigate the role of the WWOX tumour suppressor gene in neuroblastoma formation. We performed real-time RT-PCR to analyse levels of WWOX expression in 22 neuroblastic tumour samples in correlation with genes involved in cell cycle regulation (CCNE1, CCND1), proliferation (MKI67), apoptosis (BCL2, BIRC5, BAX) and signal transduction (EGFR, ERBB4). We also evaluated two potential mechanisms - promoter methylation (MethylScreen method) and loss of heterozygosity (LOH) status, which could be connected with regulation of WWOX gene expression. We found a positive correlation between WWOX gene and BCL2 and HER4 JM-a and negative with cyclin D1 and E1. Our observations are consistent with previous findings and emphasise the role of WWOX in cell cycle and apoptosis regulation. Moreover, strong positive association with HER4 JM-a in this tumour type may indicate a role for WWOX in neuroblastoma cell differentiation. The presented results indicate that LOH in locus D16S3096 (located in intron 8) may be involved in the regulation of WWOX mRNAexpression. However, no association between methylation status of WWOX promoter and its expression was observed.
Small intestinal bacterial overgrowth in patients with progressive familial intrahepatic cholestasis.
Authors: Lisowska Aleksandra , Kobelska-Dubiel Natalia , Jankowska Irena , Pawłowska Joanna , Moczko Jerzy , Walkowiak Jarosław
Published: February 2019

To date, no studies concerning the presence of small intestinal bacterial overgrowth in patients with progressive familial intrahepatic cholestasis were published. Based upon characteristic of progressive familial intrahepatic cholestasis one can expect the coexistence of small intestinal bacterial overgrowth. The aim of the study was to assess the incidence of small intestinal bacterial overgrowth in patients with progressive familial intrahepatic cholestasis.
26 patients aged 8 to 25 years with progressive familial intrahepatic cholestasis were included in the study. Molecular analysis of ABCB11 gene was performed in the vast majority of patients. In all patients Z-score for body weight and height, biochemical tests (bilirubin, bile acid concentration, fecal fat excretion) were assessed. In all patients hydrogen-methane breath test was performed.
On the basis of first hydrogen-methane breath test, diagnosis of small intestinal bacterial overgrowth was confirmed in 9 patients (35%), 5 patients (19%) had borderline results. The second breath test was performed in 10 patients: in 3 patients results were still positive and 2 patients had a borderline result. The third breath test was conducted in 2 patients and positive results were still observed. Statistical analysis did not reveal any significant correlations between clinical, biochemical and therapeutic parameters in patients with progressive familial intrahepatic cholestasis and coexistence of small intestinal bacterial overgrowth.
Our results suggest that small intestinal bacterial overgrowth is frequent in patients with progressive familial intrahepatic cholestasis. Moreover, it seems that this condition has the tendency to persist or recur, despite the treatment.
Enzymatic profile, adhesive and invasive properties of Candida albicans under the influence of selected plant essential oils.
Authors: Budzyńska Aleksandra , Sadowska Beata , Więckowska-Szakiel Marzena , Różalska Barbara
Published: February 2019

The influence of essential oils (EOs) used at sublethal level, on the presence and intensity of Candida albicans virulence factors was evaluated. Minimal inhibitory concentrations (MICs) of Lemon balm, Citronella, Geranium and Clove oils were established as 0.097% (v/v). Using the agar plates with substrates for proteases, phospholipases and hemolysins it was shown that C. albicans ATCC 10231 and C. albicans ATCC 90028 strains differed in the type and amount of enzymes produced. No significant difference in their total amount could be detected after pretreatment for 24 h with EOs at ½ MIC. However, the short-term (1 h) acting oils at MIC caused a statistically significant reduction in this activity. In the API ZYM test it was demonstrated that both strains exhibited activity of the same 9 out of 19 enzyme types and that EOs caused a significant decrease in the release of some of them. In the presence of subMIC of EOs, or when the fungus had previously been exposed to the MIC of oil, germ tubes formation was significantly and irreversibly reduced. Such C. albicans spotted on the Spider agar containing EOs at subMICs were unable to penetrate the agar. A significant decrease in the C. albicans adhesion to the fibroblast monolayer with respect to controls was also demonstrated when yeasts had been exposed to EOs at MIC (1 h) in liquid medium. Thus, it has been shown that tested oils, used even at subMIC, exhibit significant activity reducing the presence/quantity of important C. albicans virulence factors.
The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii.
Authors: Chmiel Elżbieta , Palusinska-Szysz Marta , Zdybicka-Barabas Agnieszka , Cytryńska Małgorzata , Mak Paweł
Published: February 2019

Among Legionella species, which are recognized to be pathogenic for humans, L. gormanii is the second prevalent causative agent of community-acquired pneumonia after L. pneumophila. Anti-L. gormanii activity of Galleria mellonella hemolymph extract and apolipophorin III (apoLp-III) was examined. The extract and apoLp-III at the concentration 0.025 mg/ml caused 75% and 10% decrease of the bacteria survival rate, respectively. The apoLp-III-induced changes of the bacteria cell surface were analyzed for the first time by atomic force microscopy. Our studies demonstrated the powerful anti-Legionella effects of the insect defence polypeptides, which could be exploited in drugs design against these pathogens.
The amide of galacturonic acid with lysine as an immunodominant component of the lipopolysaccharide core region from Proteus penneri 42 strain.
Authors: Palusiak Agata , Maciejewska Anna , Ługowski Czesław , Różalski Antoni
Published: February 2019

Most Proteus lipopolysaccharides (LPSs) contain uronic acids or their amides with different amino acids, which together with other negatively charged components account for the acidic character of such LPS molecules. Previous studies have shown the significance of an amide of galacturonic acid with lysine [D-GalA(L-Lys)] for serological specificity of O-antigens from few P. mirabilis strains. In this work, the immunodominant role of GalALys was indicated for the P. penneri 42 LPS core region. The studies also showed the serological identity of core oligosaccharides from P. penneri 42 (O71), P. mirabilis 51/57 (O28) and R14/S1959 strains.
Type II thioesterase ScoT is required for coelimycin production by the modular polyketide synthase Cpk of Streptomyces coelicolor A3(2).
Authors: Kotowska Magdalena , Ciekot Jarosław , Pawlik Krzysztof
Published: February 2019

Type II thioesterases were shown to maintain efficiency of modular type I polyketide synthases and nonribosomal peptide synthetases by removing acyl residues blocking extension modules. We found that thioesterase ScoT from Streptomyces coelicolor A3(2) is required for the production of the yellow-pigmented coelimycin by the modular polyketide synthase Cpk. No production of coelimycin was observed in cultures of scoT disruption mutant. Polyketide production was restored upon complementation with an intact copy of the scoT gene. An enzymatic assay showed that ScoT thioesterase can hydrolyse a 12-carbon acyl chain but the activity is too low to play a role in product release from the polyketide synthase. We conclude that ScoT is an editing enzyme necessary to maintain the activity of polyketide synthase Cpk. We provide a HPLC based method to measure the amount of coelimycin P2 in a culture medium.
Vaccinium myrtillus leaves and Frangula alnus bark derived extracts as potential antistaphylococcal agents.
Authors: Sadowska Beata , Paszkiewicz Małgorzata , Podsędek Anna , Redzynia Małgorzata , Różalska Barbara
Published: February 2019

Due to constantly increasing antibiotic resistance of pathogens and participation of the biofilms they make in various types of infections, a development of alternative therapeutic strategies becomes an urgent need. Taking advantage of the biological activity of plant-derived compounds can solve this problem. In this study antimicrobial, including those synergistic with classic antibiotics, and cytotoxic properties of newly-obtained extracts from Vaccinium myrtillus leaves (VLE) and Frangula alnus bark (FBE) were evaluated. Both tested extracts exhibited relevant antistaphylococcal activity (MIC range 0.75-1.5 mg/mL) accompanied by a relativly low cytotoxic effect on mammalian cells (BI > 1). Phytochemical analysis of the extracts tested showed a high total content of phenolic compounds with the predominance of hydroxycinnamic acids in VLE and hydroxybenzoic acids and flavanols in FBE. Widely described in the literature antimicrobial properties of phenolics were probably connected with the biological activity of the extracts tested. We also report that the presence of VLE or FBE at sub-MIC concentrations enhances biocidal potential of vancomycin and linezolid. Therefore, we are considering a possibility of an alternative therapy for local infections caused by S. aureus by combining classic antibiotics with plant-derived extracts.
Frequencies of HLA-DQ2 and HLA-DQ8 haplotypes in Czech and Slovak coeliac patients and the healthy population.
Authors: Wroblova Katerina , Kolorz Michal , Pav Igor , Horakova Zuzana , Filipova Petra , Bartos Milan , Bartosova Ladislava
Published: February 2019

Coeliac disease is an autoimmune disorder with genetic predisposition. The aim was to determine the frequency of HLA-DQ2 and HLA-DQ8 in Czech and Slovak patients and the healthy population. The study included 127 patients and 66 healthy volunteers. HLA-DQ2 was identified in 85.03% patients, and 24.24% healthy individuals (P=0.0001; OR17.7632; CI=8.4347-37.4088). HLA-DQ8 was identified in 11.81% patients and 15.5% healthy individuals. HLA-DQ8 occurred more often in HLA-DQ2-negative patients compared to HLA-DQ2-positive patients (P=0.0494; OR3.5; CI 1.0428-11.7468). At least one of the studied HLA-variants was found more often in patients than in healthy individuals (P=0.0001; OR58.8; CI 7.6856-449.8602).
Wolf Parkinson White sendromu (WPW) ablasyonu yapılan hastaların özellikleri: Tek merkez vaka serisi
Authors: KANAT Selcuk , TÜTÜNCÜ Ahmet
Published: February 2019

Amaç:
Aksesuar yol varlığı ile oluşan atriyoventriküler reentran taşikardiler (AVRT)
paroksismal supraventriküler taşikardilerin yaklaşık dörtte birini oluşturur.
Bizim retrospektif çalışmamızın amacı katater ablasyon işlemi yapılan yüksek
volümlü bir merkezde, AVRT ablasyon yapılan
hastaların demografik özellikleri, etiyolojik faktörler ve komplikasyon
oranları hakkında bilgi vermektir. Gereç ve Yöntemler:
Hastanemizin kardiyoloji kliniğinde Ocak 2014 ve Ocak 2018 yılları arasında SVT ablasyonu uygulanan 1107 hasta tarandı ve katater
ablasyon yapılmış 232 WPW ’li hasta çalışmaya dahil edildi. Bu hastalar
demografik özellikleri, etiyolojik faktörleri, aksesuar yol lokalizasyonları,
işlem başarısı ve komplikasyon gelişimi açısından değerlendirilmiştir.     Bulgular:
Hastaların yaş ortalaması 38 ±16,1 olup ve kadın cinsiyet oranı %37.1 dir. En
sık görülen lokalizasyonun sol aksesuar kaynaklı yolak olduğu görüldü(%53,1).
Tüm lokalizasyonlar için akut dönemde işlem başarısı %93,1 saptandı. Tüm
hastalarda karşılaşılan komplikasyon oranı % 3,3 iken, en sık karşılaşılan
komplikasyon girişim yeri hematomu olarak karşımıza çıkmıştır(% 2,1).  

Tartışma: Tüm
yaş gruplarını etkileyebilen ve yaşam kalitesini oldukça kötüleştirebilen bir
hastalık grubu supraventriküler taşikardiler (SVT) dir. Bu grubun ikinci
sıklıkta görülen üyesi AVRT’nin bilinen küratif tedavisi radyofrekans katater
(RF) ablasyondur. Ablasyon tedavisi yapan hekim sayısı ve işlemin yapıldığı
merkez sayısı giderek artış göstermektedir. Özellikle gelişebilecek komplikasyonlar
açısından dikkat çekmek amaçlı AVRT ablasyon tecrübelerimiz bu çalışma ile sunulmuştur.
Effect of lethal yellow (AY) mutation and photoperiod alterations on mouse behavior
Authors: Bazhenova E. Y. , Fursenko D. V. , Khotskin N. V. , Sorokin I. E. , Kulikov A. V.
Published: February 2019

Decrease in natural illumination in fall/winter months causes depressive-like seasonal affective disorders in vulnerable individuals. Obesity is another risk factor of depression. The lethal yellow (AY) mutation causes ectopic expression of agouti protein in the brain. Mice heterozygous for AY mutation (AY/a) are obese compared to their wild-type littermates (a/a). The main aims of the study were to investigate the effects of AY mutation, photoperiod and the interaction between these factors on daily activity dynamics, feeding, locomotor and exploratory activities, anxiety-related and depressive-like behaviors in mild stress condition. Six weeks old mouse males of AY/a and a/a lines were divided into four groups eight animals each and exposed to long- (14 h light and 10 h darkness) or short- (4 h light and 20 h darkness) day conditions for 28 days. Then the behavior of these mice was successively investigated in the home cage, open field, elevated plus-maze and forced swim tests. We did not observed any effect of AY mutation on the general activity, water and food consumption in the home cage; locomotion and exploration in the open field test; anxiety-related behavior in the open field and elevated plus-maze tests. At the same time, AY mutation increased depressive-like immobility time in the forced swim test (F1.28 = 20.03, p = 0.00012). Shortday conditions decreased nocturnal activity in the home cage, as well as locomotion (F1.28 = 16.33, p = 0.0004) and exploration (F1.28 = 16.24, p < 0.0004) in the open field test. Moreover, short-day exposition decreased time spent in the center of the open field (F1.28 = 6.57, p = 0.016) and in the open arms of the elevated plus-maze (F1.28 = 12.08, p = 0.0017) tests and increased immobility time in the forced swim test (F1.28 = 9.95, p = 0.0038). However, no effect of the interaction between AY mutation and photoperiod on immobility time in the forced swim test was observed. Therefore, short-day photoperiod and AY mutation increased depressive-like behavior in the forced swim test by means of different mechanisms.
Gökhöyük Tarım İşletmesi Arazilerinin İdaresinde Jeoistatistik ve Coğrafi Bilgi Sistemleri Tekniklerinin Kullanımı
Authors: BUDAK Mesut , ACİR Nurullah
Published: February 2019

Arazi kullanımı, ana materyal ve topoğrafyaya bağlı olarak büyük
değişkenlik gösteren toprak özelliklerinin mesafeye bağlı değişkenlikleri;
toprağın verimliliği, kalitesi ve genel olarak sürdürülebilirliğini önemli
düzeyde etkilemektedir. Bu çalışma; yaklaşık 1900 hektar genişliğindeki
Gökhöyük Tarım İşletmesi arazilerinin bir kısım toprak özelliklerinin mesafeye
bağlı değişkenliklerini belirlemek, haritalamak ve işletme arazilerinin
sürdürülebilir kullanımlarını etkileyecek sorunların tespit edilerek çözüm
önerilerini ortaya koymak amacı ile gerçekleştirilmiştir. Bu amaçla, çalışma
alanını temsil edecek şekilde 63 noktadan ve 4 farklı derinlikten (0-30, 30-60,
60-90 ve 90-120 cm) toprak ve 19 noktadan da taban suyu örnekleri alınmıştır.
Toprak örneklerinde elektriksel iletkenlik (EC), pH, kil, kum ve silt
içerikleri ile hidrolik iletkenlik değerleri; taban suyu örneklerinde ise pH ve
EC değerleri belirlenmiştir. Klasik istatistik ve jeoistatistik yöntemler ile
çalışılan özelliklerin, arazideki genel durumu ve mesafeye bağlı
değişkenlikleri karakterize edilmiştir. Araştırma sonucuna göre, yüksek kil
içeriğine sahip olan hem yüzey hem de yüzey altı topraklarında hidrolik
iletkenlik değerleri (<20 mm h-1) oldukça düşük bulunmuştur. Bitki
besin elementi alımı üzerine önemli bir etkisi olan pH değerlerinin tüm
derinliklerde ve arazinin önemli bir kısmında 8.5’in üzerinde; çalışma alanının
orta kısmında bir hatta yer alan toprakların 60-120 cm derinliğinde EC
değerleri (>4 dS m-1) sorun oluşturabilecek düzeylerde olduğu
tespit edilmiştir. Bu bölgedeki su örneklerinin de EC değerleri 20 dS m-1’nin
üzerinde olduğu görülmüştür. Kurak ve sıcak dönemlerde tuz içeriği yüksek taban
suyunun kapilarite ile yüzeye taşınması, toprağın üretkenlik fonksiyonuna zarar
verebilir. Toprak özelliklerinin mesafeye bağlı değişimini gösteren haritaların
kullanımı ile çiftlik arazisinde bitkisel üretimin geliştirilmesine ve toprak
kalitesinin iyileştirilmesine katkı sağlayacak kararların doğru bir şekilde
alınması mümkün olabilecektir.
Syrian Refugee Families with Young Children: An Examination of Strengths and Challenges During Early Resettlement
Authors: Yohani Sophie , Brosinsky Larissa , Kirova Anna
Published: February 2019

With the arrival of a large number of Syrian families to Canada, educators and other service providers are reflecting on best practices to support the psychosocial adaption of refugees from conflict settings. This article draws on a study that examined the psychosocial adaptation of Syrian refugee families with young children in Western Canada, and uses the RAISED Between Cultures framework to discuss their strengths and identified barriers during early resettlement. Using a community-based participatory research approach and critical incident method, the study involved focus groups and semi-structured interviews with ten Arabic-speaking cultural brokers who were working with Syrian refugee families using holistic supports during early resettlement. Data were analyzed thematically both across and within 10 cases, then examined in light of six factors that contribute to refugee children’s outcomes as identified in the RAISED Between Cultures framework. As key figures in refugee children and families’ adaptation to their host country, educators can draw on these findings to identify families’ and children’s’ strengths and challenges during early resettlement to ensure positive child outcomes.
Language and Identity Development Among Syrian Adult Refugees in Canada: A Bourdieusian Analysis
Authors: Ghadi Needal , Massing Christine , Kikulwe Daniel , Giesbrecht Crystal
Published: February 2019

Framed by Bourdieu’s work, this article focuses on the intersections between language learning experiences, capital, and identities of Syrian refugees now living in Regina, Saskatchewan. In this qualitative study, data were collected during a series of focus groups with Syrian women and men. Based on the study findings, we contend that the participants’ multiple identities as hard-working, employed, independent, Muslim mothers or fathers, and wives or husbands developed in Syria were gradually eroded or altered by the realities they experienced in Canada, yet they had a strong desire to re-establish their identity constructions from back home in the new context. We assert that the loss of their linguistic capital from back home limited their employment prospects, impacted their abilities to form social relationships with native English speakers, and led to a shift in traditional gender roles. It is imperative to adapt language training programs in order to support refugees in re-establishing themselves in their professional fields and daily living activities.
Label Identification And Bacteriological Quality Assessment Of Sambel Pecel (Peanuts Dressing Sauce) Product In Madiun City
Authors: Fadhilah Richa Dwi
Published: February 2019

Food product requirements before distribution to consumers are including of the proper food labeling, and zero count of Eschericia coli bacteria. This study aims to identify the labeling process  and bacteriological examination (Eschericia coli) on javanese peanut dressing sauce (sambel pecel) product in Madiun city. The research is an observational research whereas data is analyzeddescriptively by cross sectional study design. This study used observations andinterview in obtaining data regarding the completeness of labeling. bacteriological examination (Eschericia coli) was performed by Environmental Health Laboratory of Health Polytechnic of Ministry of Health Surabaya for the total sample of 15 sambel pecel products. All of sambel pecel products in Madiun are without a complete label andwere inconsistent with the standards of the Governmental Regulations of food label and advertisement regulation.Each product is required to implement eleven label component such as product name, composition, net weight, expiration date, name and address of producer, label of halal, and consument information for disability occurs. Five out of 15 products were positively contain Eschericia coli bacteria while the products based on regulation about food, that it must be 0 (zero). Thus it can be suggested for Trade and Industry Service of Madiun toimplement stringent selection and monitoring process before distribute the product to general population.
Comparison of the effect of sambiloto (AS 201-01) tablet and dihydroartemisinin-piperaquine on macrophage MIF expression in mice placenta infected with Plasmodium berghei
Authors: Aryani Desak Ketut Ayu , Prasetyo Budi , Widyawaruyanti Aty , Widjiati Widjiati
Published: February 2019

Objective: To compare the administration of sambiloto tablets (AS201-01) and dihydroartemisinin-piperaquine phosphate tablets in pregnant mice infected by P. berghei on the expression of MIF in the placenta.Materials and Methods: Experimental laboratory research, 24 pregnant mice were divided into 4 groups with randomization, ie. non-infected, placebo, sambiloto (AS201-01) and DHP groups. On day 9 P. berghei was infected, on day 11 the treatment was given, day 15 the surgery was performed, placental samples were taken, immunohistochemical staining was given, and MIF expression was assessed.Results: The expression of MIF in the group not infected with P. berghei had the lowest mean, while the highest mean was found in the placebo group. Uninfected groups were not significantly different from sambiloto (AS201-01) tablet group. Sambiloto tablet group (AS201-01) had lower MIF expression than DHP group, but it was not significantly different. Sambiloto tablet group (AS201-01) showed lower MIF expression than placebo. MIF expression in DHP group was lower than that in placebo group. From the lowest, the MIF expressions were as follows: group not infected with P. berghei, group receiving sambiloto (AS201-01) tablet, DHP group and placebo group.Conclusion: MIF expression in the placenta of pregnant mice infected with P. berghei and receiving sambiloto (AS20-01) tablets was not different from those receiving DHP tablets.
Role of aspirin dose in reducing uterine artery resistance in 16-24 weeks pregnant women with abnormal uterine artery resistance
Authors: Adibrata Muhammad Arief , Sulistyono Agus , Ernawati Ernawati
Published: February 2019

Objectives: To compare the decrease of resistance index (RI) from uterine artery in pregnant woman receiving low dose aspirin therapy between 80 mg/day and 125 mg/day who had abnormal doppler velocimetry (DV) ultrasound examination at 16-24 weeks.Materials and Methods: An experimental study using double blind randomized clinical trial design. Subjects were from Mulyorejo and Kalijudan public health service in Surabaya, that included pregnant women with 16-24 weeks of pregnancy with abnormal uterine artery velocimetry ultrasound. The results of ultrasound Doppler examination were divided into four levels; normal (RI<0.58; (-) diastolic notching), level I (RI> 0.58; (-) diastolic notching), level II (RI<0.58; (+) disatolic notching) and level III (RI> 0.58; (+) diastolic notching). Uterine doppler ultrasound examination was performed at Fetomaternal Division, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Hospital, Surabaya, Indonesia, by fetomaternal consultants before and after the admin-istration of low-dose aspirin of 125 mg/day and 80 mg/day for four weeks.Results: Ninety subjects were obtained and randomized into 2 groups, with 45 subject in each group. Before treatment, in 125 mg/day group those with level I were 34 subjects and level III 11 subjects. In 80 mg/day group, level I 41 subjects, level II 2 subjects and level III 2 subjects. After 4 weeks of treatment, a second DV USG was performed in Aspirin 125 mg/day group. Normal were 40 subjects, level I 4 subjects, and level III 1 subject. In aspirin group 80 mg/day, normal 22 subjects, level I 19 subjects and level III 4 subjects. The analysis was performed with Wilcoxon test before and after treatment in both aspirin treatment group 125 mg/day and 80 mg/day with p value respectively, p=0.001 and p=0.005.Conclusion: Compared with aspirin of 80 mg/day, aspirin of 125 mg/day is more superior to decrease uterine arterial resistance in pregnant women with ultrasound uterine arterial doppler velocimetry at 16-24 weeks gestational age.
Validasi Metode Bioanalisis Vankomisin HCl dalam Spiked-plasma Manusia Menggunakan KCKT-UV untuk Aplikasi PKOD
Authors: Wibowo Ari , Maulidina Damas Inggil , Fitri Wahyuni Shalatan , Ningrum Vitarani
Published: February 2019

As the first-line antibiotic for the treatment of infections caused by methicillin-resistant Staphylococcus aureus  (MRSA), vancomycin has  a narrow therapeutic index with high pharmacokinetic   variability. Therefore, it is deemed necessary to examine its concentration in the blood as a strategy to monitor the fulfillment of therapeutic levels  in patients receiving vancomycin. This study aimed to validate vancomycin bioanalysis  in  spiked-human  plasma  for  the  applications  of  therapeutic  drug  monitoring  (TDM).
PENGARUH KUALITAS PRODUK, PERSEPSI HARGA, DAN CITRA MEREK TERHADAP KEPUASAN DAN LOYALITAS PELANGGAN BETON SIAP PAKAI HOLCIM DI JAKARTA
Authors: Budiastari Sita
Published: February 2019

ABSTRAK

 

Tujuan penelitian ini adalah untuk mengetahui gambaran atas pengaruh kualitas produk, persepsi harga dan citra merek terhadap kepuasan dan loyalitas pelanggan beton siap pakai di Jakarta. Penelitian ini adalah penelitian kuantitatif dengan metode deskriptif dan kausal yang di lakukan di Holcim Beton pada tahun 2013 dengan sampel sebanyak 100 pelanggan di Jakarta dan sekitarnya pada segmen Industrial dan komersial proyek. Pengumpulan data responden dengan kuesioner dan dianalis dengan menggunakan analisa jalur (path analysis) dengan bantuan program SPSS versi 20.0. terhadap kepuasan pelanggan (2) Persepsi Harga berpengaruh positif dan signifikan tehadap kepuasan pelanggan, (3) Citra Merek tidan memiliki pengaruh terhadap Hasil penelitian disimpulkan bahwa (1) Kualitas Produk tidak berpengaruh kepuasan pelanggan, (4) semua variabel bebas, kualitas produk, Persepsi harga dan Citra Merek berpengaruhi positif dan signifikan terhadap variabel terikat Kepuasan Pelanggan, dengan nilai determinasi sebesar 50 %, sedangkan 50% lainnya di pengaruhi oleh variabel lainnya (5) Kualitas Produk berpengaruh dan signifikan terhadap Loyalitas Pelanggan melalui kepuasan pelanggan, (6) Persepsi Harga tidak berpengaruh terhadap loyalitas melalui kepuasan pelanggan, (7) Citra Merek berpengaruh dan siginikan terhadap loyalitas melalui kepuasan pelanggan, (8) Semua varibel bebas kualitas produk, persepsi harga dan citra merek secara bersama-sama berpengaruh dan signifikan terhadap loyalitas melalui kepuasan pelanggan, dengan nilai determinasi sebesar 77.4 % dan 22.6 % lainnya di pengaruhi oleh faktor lainnya.

 

Hasil temuan mengarahkan untuk merekomendasikan agar Holcim  meningkatkan kualitas produk, persepsi harga dan citra merek untuk meningkatkan loyalitas melalui kepuasan pelanggan. Hal ini juga merekomendasikan kepada Holcim  untuk meneningkatakan layanan dan informasi kepada pelanggan guna meningkatkan kepuasan dan loyalitas pelanggannya.

 

Kata kunci: kualitas produk, persepsi harga,citra merek, kepuasan, loyalitas pelanggan, beton siap pakai Holcim di Jakarta.
Pengaruh Persepsi Public Relation, Kepercayaan dan Citra Merek terhadap Loyalitas Pelanggan
Authors: Putri Birgitta Amanda , Hidayati Tetra
Published: February 2019

Abstrak: Penelitian ini dilakukan untuk menguji pengaruh persepsi PR Kepercayaan dan Citra Merek terhadap loyalitas pelanggan. Penelitian ini menggunakan persamaan regresi berganda dimana data didapat dari konsumen atau pelanggan Brow Studio (perusahaan sulam alis milik Anggie Rassly). Berdasarkan kriteria yang telah ditentukan, peneliti mendapatkan 225 data yang dianalisis dengan menggunakan SPSS sebagai software pengolah datanya. Hasil penelitian ini menunjukkan bahwa persepsi PR, kepercayaan dan citra merek berpengaruh signifikan terhadap loyalitas pelanggan.

Kata kunci: persepsi PR, Kepercayaan, citra merek, loyalitas pelanggan
Safevi Kroniklerinde Çaldıran Savaşı
Authors: GENÇ Vural
Published: February 2019

Üzerinden beş yüz geçmesine rağmen Çaldıran
Savaşı (1514) sonuçları itibarıyla hala tartışılmaktadır. Osmanlı kaynakları bu
savaştan kesin bir galibiyet olarak bahsederken Safevi kaynakları bunu bir
mağlubiyet olarak görmezler. Taktik icabı geri çekiliş olarak telakki eden
Safevi kaynakları, buna uygun çeşitli gerekçeler öne sürerler. Bu makalede
modern tarihyazımında oldukça ihmal edilen Safevi kaynaklarından hareketle
Safevilerin bu savaşı nasıl algıladıklarına değinilecektir. Her iki tarafın
kaynaklarının okunması meselenin daha iyi anlaşılmasını sağlayacaktır.
KURUMSAL AHLÂK PERSPEKTİFİNDEN HASTANE AHLÂKI
Authors: ERDEM İzzet , ERDEM Ramazan
Published: February 2019

Ahlâk insanın yaratılıştan gelen özellikleri ile sonradan benimsediği huy, alışkanlık ve davranışların bütünü ifade etmektedir. Tek başına kullanımda güzel huy ve niteliklere işaret etmektedir. Kurumsal bazda ahlâka dair incelenmesi gereken kurumların başında hastaneler gelmektedir. Çünkü hastaneler birebir insan sağlığına yönelik hizmet veren kurumlardır. Hem sağlık hizmeti veren personel ile hizmet alan hastalar arasındaki etkileşim, hem de hastane personelinin kendi içinde ve dış paydaşlarla olan etkileşimi ahlâksızlık mahiyetinde bir takım sonuçlar doğurmaktadır. Türkçe literatürde kurumsal anlamda “hastane ahlâkı” kavramının yeni kullanılacak olması çalışmanın önemi açısından önemlidir. Bu çalışmada ahlâk, meslek ahlâkı ve iş ahlâkı ile ilgili kavramlar ışığında kurumsal olarak hastane ahlâkının mahiyetine ilişkin bir değerlendirme yapılması hedeflenmiştir. Buna göre hastane personelinin ve hastaların temel alındığı çift yönlü bir hastane ahlâkı yapısının mevcut olduğu söylenebilir.
FARKLI BAKIŞ AÇISI KAZANMAK İÇİN BEYİN FIRTINASI MODELLERİ
Authors: AKIN Mustafa Şeref
Published: February 2019

Einstein’ın sorunların çözümüyle ilgili önemli bir tespiti, problemlerin onları oluşturan aynı düşünce düzeyiyle çözülemeyeceği yönündedir. Öyleyse başka bir bakış açısı nasıl yakalanacak? Bunun için farklı bakış açısını çağrıştıracak metodolojik beyin fırtınası metotları mevcut mu? Bu makalede “alternatif dünyalar, zıtlıklar, 10’un gücü” beyin fırtınası metotları ile mevcut unsurlar farklı bakış açılarıyla birleştirilip yeni çözümler üretme yolları gösterilecek. Video kiralama, eğitim, bankacılık, hastane, catering, dondurulmuş gıda, ATM, birimlerin yeniden organizasyonu üzerine farklı sektörlerden örnek çalışmalar ele alınacak. İnsanları farklı düşünmeye zorlamak satrancı hiç bilmeyen bir kişiye “Rakibini mat et.” demektir. Çerçeve dışına bakılmadan önce çerçevede (ve içinde) sonrasında çerçevenin dışında nereye bakılacağının araştırılması gerekir. Beyin fırtınası öncesi ana husus problemin ve unsurların tespitidir. Bu unsurların çözümü için kaynak mekânların arayışına geçilir. Sonrasında beyin fırtınası yapılır. Rastgele iki farklı kavramın birleştirilmesi ile aşılama yapılmaya çalışılmaz.
KURUMSAL SOSYAL SORUMLULUK VE AKTİVİZM: İLİŞKİSEL DÜZLEMDE EPİSTEMOLOJİK BİR YÖNELİM
Authors: ARKLAN Ümit , KARTAL Nurullah Zafer
Published: February 2019

Yaşadığımız dönemde kurumsal vatandaşlığın gereği ve emaresi olarak önem taşıyan kurumsal sosyal sorumluluk; ekonomik, yasal, etik ve sosyal (hayırseverlik)  boyutlarıyla, sadece toplumdaki bireylerin hassasiyetlerinden ötürü değil, aynı zamanda kurumsal yapıların kazanımlar sağlayabilmek veya olumsuzlukları bertaraf edebilmek adına da girişimlerde bulunduğu bir uygulama alanıdır. Bahsi geçen kazanımlar ve olumsuzluklar noktasında temel motivasyon kaynağı niteliğindeki olgulardan biri ise, aktivizmdir. Kurumların varoluş nedenleri olan faaliyetleri gerçekleştirirken hedef kitlenin istek, beklenti ve şikayetlerine gereken ilgiyi göstermemesi, sorumluluklarını yerine getirme noktasında yeterli hassasiyeti taşımaması, onları aktivizm hareketleriyle karşı karşıya bırakabilmektedir. Hal böyle olunca, aktivizm, tüm kurumsal yapıların göz önünde bulundurarak hareket etmesi gereken bir olgu olarak gündeme gelmektedir. Bu bağlamda, çalışmada, kurumsal sosyal sorumluk boyutları ve aktivizm ilişkisini epistemolojik bir yaklaşımla ortaya koyma amacı taşınmaktadır. Kuramsal tartışmalarda betimlenen kurumsal sosyal sorumluluk ve aktivizm ilişkisinin, örnek durum çalışması olarak alınan ilk halkla ilişkiler örneklerinden Colorado kömür grevi ve son örneklerden Deepwater Horizon patlaması olaylarıyla da gerçek hayattaki tezahürü gözler önüne serilmektedir. Değerlendirmeler sonucunda, geçmişten bugüne kurumların aktivizm hareketlerini ortadan kaldırmak için, yerine getirmedikleri kurumsal sosyal sorumluluk boyutunu uygulamaya koyma ya da sorumluluk boyutlarının en gözde ve en kabul edilebilir olan sosyal (hayırseverlik) boyutuna ağırlık verme yoluna gidebildikleri bulgulanmakta ve bu durum gerekçeleriyle birlikte tartışılmaktadır.
Novel AGR-EOR Compression Integration for Process Optimization
Authors: Amhamed Abdukarem , Abotaleb Ahmed
Published: February 2019

In this work we aim to enhance the sour-gas loading in acid-gas removal (AGR) systems, maximizing oil-production rate at the tertiary phase and enhanced oil recovery (EOR), and mitigating vented carbon dioxide (CO2) with minimal modification to the existing systems. We conducted a simulation study on the basis of a real natural-gas liquids (NGLs) plant and Qatari oil wells with a 390-MMscf/D feed of sour gas using HYSYS and ProMax process simulation tools to evaluate the novel configurations compared with a conventional AGR system.

The results show that the acid-gas loading improved from 0.48 to 0.81, and the amine circulation rate decreased by 40%, while maintaining the treated-gas quality specifications (4 ppm H2S, 1 mol% CO2). The required CO2 compression power for CO2-EOR decreased by 15.49%, and the oil production was enhanced by 1,360 B/D. In addition, 13.6 MMscf/D of CO2 is mitigated and used rather than vented.
Propofol attenuated the effect of TNF-α on occludin expression by inhibiting HIF-1α/VEGF/VEGFR-2/ERK signaling pathway in hCMEC/D3 cells
Authors: Zhang Yue , Ding Xiaowei , Miao Changhong , Chen Jiawei
Published: February 2019

Purpose

The levels of tight junction proteins (TJs), especially occludin correlate with blood-brain barrier (BBB) disruption caused by central nervous system (CNS) inflammation during perioperative period. It has been reported that propofol, the most commonly used anesthetic, could inhibit inflammation response in CNS. In this study, we investigated the effects of TNF-α and propofol on occludin expression in human cerebral microvascular endothelial cell line, D3 clone (hCMEC/D3 cells), and explored the underlying mechanisms.

Methods

The hCMEC/D3 cells were treated with propofol, followed by TNF-α. The expression and phosphorylation of HIF-1α, VEGF, VEGFR-2, ERK and occludin were measured by Western blot analysis.

Results

TNF-α (10 ng/ml, 4 h) significantly decreased the expression of occludin, which was attenuated by propofol (25 μM). TNF-α induced HIF-1α/VEGF/VEGFR-2/ERK signaling pathway, while propofol could inhibit it. In addition, the inhibitors of HIF-1α, VEGF, VEGFR-2, and ERK could reduce the effect of TNF-α on occludin expression.

Conclusion

TNF-α could decrease the expression of occludin via HIF-1α/VEGF/VEGFR-2/ERK signaling pathway, which was attenuated by propofol.
Electric vagal nerve stimulation inhibits inflammation and improves postoperation cognitive dysfunction in aged rats
Authors: Xiong Jun , Wang Huijun , Sun Yongxing
Published: February 2019

Objective This study aimed to evaluate effects of vagal nerve stimulation on postoperation cognitive dysfunction in aged rats.
Material and Methods: A total of 33 male SD rats were ssigned randomly to three groups, the control group, the s group and sv group. Behavior and memory were evaluated by Open Field Test and Morris Water Maze. The cytokines level were measured by ELISA. The TNF-α protein in hippocampal zone were assessed by Western blotting. qPCR was used to detected the mRNA expression of NF-κB in hippocampus.
Results: During the anesthesia/operation, the vital life signs of rats were stable. In SV group, vagal nerve stimulation could decrease heart rate lower than 10% of basic level and kept it at a stable range by regulation of stimulation intensity. After stimulation stop, the heart rate would return to the basic level again. This indicated that the model of vagal nerve stimulation was successful. The serum inflammatory cytokines of TNF-α and IL-6 increased by the operation/anesthesia, but these cytokines could be decreased by the vagal nerve stimulation (all P<0.05). The level of TNF-α protein and mRNA expression of NF-κB in hippocampus were also eliminated by the vagal nerve stimulation compared to S group (P<0.05). The Morris Water Maze results showed the escape latency of postoperation in the S group was significantly longer than the C group (P<0.05), and the times of crossing platform in the S group was lower than that of the C group (P<0.05). Although the escape latency of postopration in the SV group was shorter than that of the S group, there was no significant difference between two groups. There were no significant differences in behavior test by Open Field test between three groups.
Conclusion The inflammation caused by the operation and general anesthesia was an important reason of postoperation cognitive dysfunction, and electric vagal nerve stimulation could inhibit this inflammation. Meanwhile, vagal nerve stimulation could ameliorate postoperation cognitive dysfunction partly, but the protective effects were not enough and should be studied and improved in the future.
Dexamethasone for the prevention of postoperative nausea and vomiting in children undergoing non-cardiac surgery: a systematic review and meta-analysis
Authors: Shen Qihong , Li Hui-fang , Zhou Xu-yan , Yuan Xiao-zong
Published: February 2019

BACKGROUND: Postoperative nausea and vomiting (PONV) is a frequent and distressing complication of general anaesthesia in paediatric patients. Dexamethasone was reported to prevent PONV in previous randomized controlled trials (RCTs). The purpose of this meta-analysis was to evaluate the role of dexamethasone in the prevention of PONV in children undergoing non-cardiac surgery.
METHODS: PubMed, EMBASE, the Cochrane Library, and Web of Science were searched to identify randomized trials that reported the efﬁcacy of perioperative dexamethasone in paediatric non-cardiac surgical patients.
RESULTS: Fourteen randomized controlled trials that included 1700 children were included. Compared with placebo, dexamethasone showed a lower incidence of PONV by intravenous injection (RR 0.38, 95% confidence interval (CI) 0.30–0.49) and by local infiltration (RR 0.58, 95% CI 0.34–0.99). The combination of dexamethasone and a 5-HT3 receptor antagonist was more effective than the 5-HT3 antagonist or dexamethasone alone in preventing PONV.
CONCLUSION: Perioperative dexamethasone was an effective measure with few complications for preventing PONV in children after non-cardiac surgery.
Influence of proinflamatory macrophages (M-1) on proliferative activity and PPAR-γ expression in neoplastic rat hepatocytes in vitro
Authors: WÓJCIK MARTA , KOSIOR-KORZECKA URSZULA , PLEWIK MICHAŁ , BOBOWIEC RYSZARD
Published: February 2019

We sought to analyse the proliferative activity and PPARγ expression in neoplastic and non-neoplastic rat hepatocytes, exposed to immunologically trained macrophages M1 (Mf-M1). Ten-week-old female Wistar rats were divided into two groups: I - control (n=5) and II neoplstic (n=5). To induce HCC in the neoplastic group, genotoxic diethylnitrosamine (DEN) was administered after a partial hepatectomy (PH). Hepatocytes were isolated by liver perfusion method and the mononuclear blood cells were isolated using Lymphoprep density-gradient centrifugation. After differentiation, blood cells were treated with barley-derived β-glucan (BBG) (10 μg/ml). Adhered heaptocytes and Mf were cultured in 3D Quasi-Vivo System during 24 h. Then, hepatic proliferation and PPAR γ expression were analysed after 72 h and the 1st ,the 2nd and the 3rd week of incubation. The proliferation index of control hepatocytes ranged between 0.41±0.04 – 0.43±0.03 after 72 h and after the 3rd week of incubation respectively. Exposure of these cells to Mf resulted in marked increase of IP after the 1st, the 2nd and the 3rd week of incubation. When hepatocytes were influenced by proinflammatory Mf- M1, their proliferation was maintained at control stage. DEN-obtained hepatocytes, not influenced by macrophages, exhibit enhanced proliferation. When these cells where co-cultured with Mf-M1, marked (P≤0.05) inhibition of cell proliferation was observed. In such condition, a high negative relationship (r= -0.93) between proliferative activity of the hepatocytes and the PPARγ concentration was observed. We conclude that immunologically trained macrophages M1, are capable to activation of PPARγ in rat neoplastic hepatocytes derived from experimentally induced HCC. In turn intensified expression of PPARγ may inhibited proliferation of neoplastic cell in vitro.
Inhibition effect of dissolved organic matter in secondary-treated municipal wastewater on corrosion of N80 carbon steel
Authors: Shen Zhanhui , Zhang Shuzhan , Dong Xingyi , Shi Jialu , Fan Jing , Li JianSheng
Published: February 2019

Secondary-treated municipal wastewater (MWW) is the alternative water source in recirculating cooling water systems. Municipal wastewater treatment plant (WWTP) effluent was collected and four fractions including hydrophobic acidic (HPOA), hydrophobic alkaline (HPOB), weakly hydrophobic (WHPO) and hydrophilic (HPI) organics were isolated from the effluent by using DAX-8/XAD-4 resin. We used potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) to investigate corrosion characteristics of N80 carbon steel in the different water samples and control samples. In contrast with control samples, the corrosion rate of N80 carbon steel decreased 41%, 23%, 18% and 4% in the presence of HPOA, HPOB, WHPO and HPI, respectively. The results were further confirmed by the EIS test. In the presence of the four fractions (especially HPOA), the radius of the semicircle in the Nyquist plot was much larger than that for the control samples, which indicated that the impedance in the EIS test of the HPOA sample was much higher than that of the control sample. The dissolved organic matter (DOM) could adsorb onto the surface of the electrode and form a protective film, which could inhibit electron transfer and increase the electrochemical impedance of the electrochemical test system.
Article Reviews: How Safe Is Surgery for PAH Patients?
Authors: Preston Ioana
Published: February 2019

Summaries and commentaries from the section editors and invited reviewers present a clinical context for practitioners' application of the latest published research relevant to the care of patients with pulmonary hypertension. In this issue Ioana Preston, MD, discusses a recently published international, prospective survey of patients with PAH undergoing noncardiac and nonobstetric major surgery.
Preoperative Considerations in Patients With Pulmonary Hypertension: Your Patient Needs Surgical Clearance
Authors: Studer Sean M.
Published: February 2019

Patients with pulmonary arterial hypertension (PAH) are at increased morbidity and mortality risk when facing the need to undergo surgical interventions. The most common complications include those arising from right ventricular (RV) failure and respiratory failure—not surprising given the complex cardiopulmonary pathophysiology of this disease. While data are limited regarding the optimal preoperative approach to these patients, it is imperative to focus on the following key components: ensuring or establishing the patient's World Health Organization (WHO) classification, pulmonary hypertension (PH) group or subgroup; assessing the status and stability of RV function; optimizing the treatment regimen; and communicating a management plan for intra- and perioperative management to all members of the interdisciplinary clinical team. This article will focus on each of these steps in the preoperative algorithm, highlighting the need for further studies in this area.
PHPN: The Many Faces of the PH Professional Network
Authors: Bartlett Mary , Stewart Traci , Wilson Melisa
Published: February 2019

Section Editor's Note: Our authors for the following Pulmonary Hypertension Professional Network (PHPN) article are both leaders within our organization as well as veterans to the field of pulmonary hypertension. Traci Stewart is a nurse clinician specialist in pulmonary hypertension and cardiomyopathy. Traci is the Immediate Past Chair of the PHPN. Melisa Wilson is a nurse practitioner specializing in pulmonary hypertension and heart failure. Melisa is the current PHPN Chair.

The design of this article gives Traci and Melisa a platform to speak about the history of the PHPN and the benefits of a PHPN membership. They will also touch on the importance of being involved and taking advantage of the opportunity to help shape our dynamic organization.