bluuebunny/crossref_metadata_2025 huggingface dataset:
Land Use and Soil Organic Carbon Stocks—Change Detection over Time Using Digital Soil Assessment: A Case Study from Kamyaran Region, Iran (1988–2018)
Authors: Nabiollahi Kamal , Shahlaee Shadi , Zahedi Salahudin , Taghizadeh-Mehrjardi Ruhollah , Kerry Ruth , Scholten Thomas
Published: March 2021

Land use change and soil organic carbon stock (SOCS) depletion over time is one of the predominant worldwide environmental problems related to global warming and the need to secure food production for an increasing world population. In our research, satellite images from 1988 and 2018 were analyzed for a 177.48 km2 region in Kurdistan Province, Iran. Across the study area. 186 disturbed and undisturbed soil samples were collected at two depths (0–20 cm and 20–50 cm). Bulk density (BD), soil organic carbon (SOC), rock fragments (RockF) and SOCS were measured. Random forest was used to model the spatial variability of SOCS. Land use was mapped with supervised classification and maximum likelihood approaches. The Kappa index and overall accuracy of the supervised classification and maximum likelihood land use maps varied between 83% and 88% and 78% and 85%, respectively. The area of forest and high-quality rangeland covered 5286 ha in 1988 and decreased by almost 30% by 2018. Most of the decrease was due to the establishment of cropland and orchards, and due to overgrazing of high-quality rangeland. As expected, the results of the analysis of variance showed that mean values of SOCS for the high-quality rangeland and forest were significantly higher compared to other land use classes. Thus, transformation of land with natural vegetation like forest and high-quality rangeland led to a loss of 15,494 Mg C in the topsoil, 15,475 Mg C in the subsoil and 15,489 Mg C−1 in total. We concluded that the predominant causes of natural vegetation degradation in the study area were mostly due to the increasing need for food, anthropogenic activities such as cultivation and over grazing, lack of government landuse legislation and the results of this study are useful for land use monitoring, decision making, natural vegetation planning and other areas of research and development in Kurdistan province.
Chemical Properties and Bacterial Community Reaction to Acidified Cattle Slurry Fertilization in Soil from Maize Cultivation
Authors: Wierzchowski Paweł Stanisław , Dobrzyński Jakub , Mazur Kamila , Kierończyk Marek , Wardal Witold Jan , Sakowski Tomasz , Barszczewski Jerzy
Published: March 2021

Acidified slurry is a novel organic fertilizer that limits gaseous ammonia emissions and reduces nitrogen losses. Our research aimed to determine the effects of short-term fertilization with acidified slurry on the chemical properties and bacterial community of soil used for maize cultivation. In the months after spreading, raw slurry fertilization had a significant impact on the increase in values of N-NO3. In contrast, soil fertilized with acidified slurry had lower N-NO3 values when compared to raw slurry fertilization treatments. Bacterial sequencing using Illumina MiSeq showed no differences in the genetic diversity of bacterial communities. In all tested soil samples, dominants at the phylum level were Actinobacteria, Proteobacteria, and Acidobacteria, while dominants at the class level were Actinobacteria, Alphaproteobacteria, Thermoleophilia, Gammaproteobacteria, and Acidimicrobiia. The values of biodiversity indices (Shannon index, Simpson index) in tested samples were similar. Our results suggest that short-term fertilization with acidified slurry does not adversely affect the biodiversity and structure of the bacterial communities and has a slight impact on soil chemical properties.
Enhancement to Salt Stress Tolerance in Strawberry Plants by Iodine Products Application
Authors: Medrano Macías Julia , López Caltzontzit María Guadalupe , Rivas  Martínez Erika Nohemi , Narváez Ortiz Willian Alfredo , Benavides Mendoza Adalberto , Martínez Lagunes Paulino
Published: March 2021

Iodine is a non-essential element for land plants, but is considered as a beneficial element, related to antioxidant capacity, environmental adaptations and improvement of plant growth. Salinity is one of the more recurrent abiotic stresses worldwide, seriously affecting vegetal production. The aim of this work was to evaluate iodine application (Q products® and KIO3, Quimcasa de México, Naucalpan, Mexico) in strawberry plants under normal and salt stress conditions. Growth, antioxidant content, essential minerals, iodine accumulation and fruit quality were evaluated. The results showed that, under stress conditions, the application of Q products increased ascorbate peroxidase (APX) and catalase (CAT) activity as well as glutathione (GSH) content and yield in fruit, without avoiding biomass loss; with the application of KIO3 an increase in GSH and APX activity as well as P and K concentrations were obtained. In leaves an increase in P, Ca, Mn and iodine accumulation was evidenced with the application of Q products, and an increased concentration of ascorbic acid and iodine with KIO3 treatments. Under normal conditions in fruits, the application of Q products increased phenolic compounds synthesis; additionally, an increase in Ca and Mn concentrations was shown. KIO3 application increased the firmness and Mn. In leaves, the application of Q products increased chlorophyll a, b and calcium. In conclusion, the application of iodine improves the quality value of strawberries under normal conditions and provides an enhancement of salt stress tolerance.
Systematic Review: Comparison of the Main Variables of Interest in Publications of Canine Bite Accidents in the Written Press, Gray and Scientific Literature in Chile and Spain, between the Years 2013 and 2017
Authors: Barrios Carmen Luz , Aguirre Valentina , Parra Alonso , Pavletic Carlos , Bustos-López Carlos , Perez Sandra , Urrutia Carla , Ramirez Josefa , Fatjó Jaume
Published: March 2021

Dog bites are a major public health problem, with consequences such as physical injury, psychological trauma, transmission of zoonoses, infections, and economic costs. For this reason, it is necessary to develop preventive programs, which require quality information to support the authorities’ decision-making and to raise public awareness about the application of the proposed measures. The objective of this review was to analyze the press, indexed and gray dog bite literature published during the 2013–2017 period. During that period, 385 articles from three sources of information were analyzed: Press literature, scientific literature, and gray literature. Of these, the greatest amount of information corresponding to the context and the aggressor animal was found in the press literature, where it was recorded that the greatest number of records reported in the Chilean articles were caused by potentially dangerous breeds (87.50%), having significant differences with the gray literature (p = 0.030), and in Spain, the greatest number of attacks was also made by potentially dangerous dogs 91.30% (21/23), statistically significant differences with the gray literature (p = 0.002) and with the indexed (p < 0.001). In the case of the scientific and gray literature, the greatest amount of information was found about the victim of the attack and the treatments applied to them. In these cases, the highest percentage of victims included in the reports contained both sexes for the two literatures (44.62% and 87.71%, respectively). Regarding the treatment applied, in the scientific literature in most of the reports, the patients received washings, rabies vaccine, and tetanus vaccine (46.26%) and presented significant differences in Chile with the information contained in the gray literature (p = 0.023), in Spain with the gray (p = 0.017) and with the press (p = 0.023). In conclusion, the press literature differs in multiple variables with the information reported in the scientific literature and, in some cases, with the gray literature. The reason why the material that is being distributed to the population would not coincide in multiple relevant variables in other literature and the representative reality of the problem is the basis for this topic.
Validation of Reference Genes via qRT-PCR in Multiple Conditions in Brandt’s Voles, Lasiopodomys brandtii
Authors: Tian Lin , Chen Yan , Wang Da-Wei , Liu Xiao-Hui
Published: March 2021

The choice of optimal reference gene is challenging owing to the varied expression of reference genes in different organs, development stages, and experimental treatments. Brandt’s vole (Lasiopodomys brandtii) is an ideal animal to explore the regulatory mechanism of seasonal breeding, and many studies on this vole involve gene expression analysis using quantitative real-time polymerase chain reaction (qRT-PCR). In this study, we used the method of the coefficient of variation and the NormFinder algorithm to evaluate the performance of nine commonly used reference genes Gapdh, Hprt1, β-actin, PPIA, Rpl13a, Tbp, Sdha, Hmbs, and B2M using qRT-PCR in eight different tissues, five developmental stages, and three different photoperiods. We found that all nine genes were not uniformly expressed among different tissues. B2M and Rpl13a were the optimal reference genes for different postnatal development stages in the hypothalamus for males and females, respectively. Under different photoperiods in the hypothalamus, none of the selected genes were suitable as reference genes at 6 weeks postnatal; β-actin and PPIA were the optimal reference genes at 12 weeks postnatal; Hprt1, β-actin, PPIA, Hmbs, and B2M were excellent reference genes at 24 weeks postnatal. The present study provides a useful basis for selecting the appropriate reference gene in Lasiopodomys brandtii.
Effect of Hydrogen Ion Presence in Adsorbent and Solution to Enhance Phosphate Adsorption
Authors: Kim Taehoon , An Byungryul
Published: March 2021

In this paper, the effect of hydrogen ions on the adsorption onto granular activated carbon (GAC) with the inorganic contaminant phosphate, which exists as a form of four species depending on the solution pH, is investigated. Various batch isotherm and kinetic experiments were conducted in an initial pH 4 as an acid, a pH 7 as neutral, and a pH 9 solution as a base for the GAC conditioned with deionized water and hydrochloric acid, referred to as GAC and GACA, respectively. The physical properties, such as the total surface area, pore volume, pore size distribution, and weight of the element, obtained from Brunauer–Emmett–Teller (BET) and scanning electron microscopy coupled with energy-dispersive X-ray spectrometry (SEM–EDX) represent no significant differences. However, the hydrochloric acid (HCl) condition results in an alteration of the pH of the point of zero charge from 4.5 to 6.0. The optimized initial pH was determined as being acid for the GAC and as being neutral for the GACA. According to the Langmuir isotherm, the relatively high Qm was obtained as being acid for the GAC and clearly distinguishes the pH effect as being the base for the GACA. An attempt was made to assess the adsorption mechanism using the pseudo-first-order (PFO), the pseudo-second-order (PSO), and the intraparticle diffusion models. The higher R2 for the PSO in the entire pH range indicated that chemisorption was predominant for phosphate adsorption, and the pH did not change the adsorption mechanism. A prolonged Bed Volume (BV) for the GACA demonstrated that the hydrogen ions on the surface of the GAC enhanced phosphate adsorption.
Cognitive Model of the Closed Environment of a Mobile Robot Based on Measurements
Authors: Pavlic Tomislav , Kušec Krunoslav , Radočaj Danijel , Britvić Alen , Lukas Marin , Milić Vladimir , Crneković Mladen
Published: March 2021

In recent years in mobile robotics, the focus has been on methods, in which the fusion of measurement data from various systems leads to models of the environment that are of a probabilistic type. The cognitive model of the environment is less accurate than the exact mathematical one, but it is unavoidable in the robot collaborative interaction with a human. The subject of the research proposed in this paper is the development of a model for learning and planning robot operations. The task of operations and mapping the unknown environment, similar to how humans do the same tasks in the same conditions has been explored. The learning process is based on a virtual dynamic model of a mobile robot, identical to a real mobile robot. The mobile robot’s motion with developed artificial neural networks and genetic algorithms is defined. The transfer method of obtained knowledge from simulated to a real system (Sim-To-Real; STR) is proposed. This method includes a training step, a simultaneous reasoning step, and an application step of trained and learned knowledge to control a real robot’s motion. Use of the basic cognitive elements language, a robot’s environment, and its correlation to that environment is described. Based on that description, a higher level of information about the mobile robot’s environment is obtained. The information is directly generated by the fusion of measurement data obtained from various systems.
New Tillage System with Additional Renovation of Soil Properties in Tramlines
Authors: Damanauskas Vidas , Jablonskytė-Raščė Danutė
Published: March 2021

Fertilizing and spraying techniques are repeatedly driven in tramlines for crop care with products whose demand increases for higher yields. The soil in tramlines is so compacted by heavy crop care machinery what leads breakdown to lifelessness. Such damaged state of the soil continues all season and leads to dejected soil productivity. The aim of this study is to find an optimal process of soil renovation in tramlines on loam and clay loam soil and to determine the required fuel consumption. The soil renovation was investigated by specially made Tiller on the dependence of tine shape types (narrow and winged) and working speed 1.6, 2.2 and 3.6 m∙s−1 in loam and clay loam soil. The beneficial relationships of soil aggregate fraction ratio, which varied from 0.54 to 4.08 and fuel consumption, which varied from 1.04 to 1.82 L·km−1 independence on tine shape and soil type was established. Based on research results, the sufficient soil loosening to suitable proportion of soil aggregates fraction ratio on loam soil was accomplished at working speed 3.6 m∙s−1 with winged tine type and fuel consumption was 1.04 L·km−1. The suitable soil aggregate fraction ratio on clay loam was achieved at working speed 3.6 m∙s−1 with narrow tine shape type and fuel consumption was 1.31 L·km−1. The volume of loosed soil in tramlines was particularly influenced by variation of tines shapes but was not influenced by the working speed majorly. The aligning of soil surface was influenced by the working speed majorly and particularly by variation of tines shapes.
Online Phase Measurement Profilometry for a Fast-Moving Object
Authors: Gao Jie , Cao Yiping , Chen Jin , Huang Xiuzhang
Published: March 2021

When the measured object is fast moving online, the captured deformed pattern may appear as motion blur, and some phase information will be lost. Therefore, the frame rate has to be improved by adjusting the image acquisition mode of the camera to adapt to a fast-moving object, but the resolution of the captured deformed pattern will be sacrificed. So a super-resolution image reconstruction method based on maximum a posteriori (MAP) estimation is adopted to obtain high-resolution deformed patterns, and in this way, the reconstructed high-resolution deformed patterns also have a good effect on noise suppression. Finally, all the reconstructed high-resolution equivalent phase shifting deformed patterns are used for online three-dimensional (3D) reconstruction. Experimental results prove the effectiveness of the proposed method. The proposed method has a good application prospect in high-precision and fast online 3D measurement.
Fracturing Fluids and Their Application in the Republic of Croatia
Authors: Gaurina-Međimurec Nediljka , Brkić Vladislav , Topolovec Matko , Mijić Petar
Published: March 2021

Hydraulic fracturing operations are performed to enhance well performance and to achieve economic success from improved production rates and the ultimate reserve recovery. To achieve these goals, fracturing fluid is pumped into the well at rates and pressures that result in the creation of a hydraulic fracture. Fracturing fluid selection presents the main requirement for the successful performance of hydraulic fracturing. The selected fracturing fluid should create a fracture with sufficient width and length for proppant placement and should carry the proppant from the surface to the created fracture. To accomplish all those demands, additives are added in fluids to adjust their properties. This paper describes the classification of fracturing fluids, additives for the adjustment of fluid properties and the requirements for fluid selection. Furthermore, laboratory tests of fracturing fluid, fracture stimulation design steps are presented in the paper, as well as a few examples of fracturing fluids used in Croatia with case studies and finally, hydraulic fracturing performance and post-frac well production results. The total gas production was increased by 43% and condensate production by 106% in selected wells including wellhead pressure, which allowed for a longer production well life.
Substitution of Material Solutions in the Operating Phase of a Building
Authors: Sobotka Anna , Linczowski Kazimierz , Radziejowska Aleksandra
Published: March 2021

During the operation of buildings, repairs, modernizations, adaptations, renovations, and reconstructions of parts of historic objects are performed. There is often the problem of using a different material or construction technology than was originally used, for a variety of reasons. For example, these are materials not currently manufactured, with necessary higher performance values (insulation, strength). The aim of the article was to analyze and evaluate the possibility of material substitution in repair works and to analyze the cause and effect analysis of its application in the context of different conditions. The article analyzes the causes and conditions of the substitution of materials in various stages of the exploitation phase of buildings, including historic buildings. A SWOT (Strengths, Weaknesses, Opportunities, Threats) matrix was developed for the phenomenon of material substitution during the operational phase. With aid from the DEMATEL (Decision Making Trial and Evaluation Laboratory) method, identification of cause–effect relationships regarding the issue of the possibility of applying the substitution of material solutions in building objects was carried out. The analysis carried out by the authors allows us to conclude that the use of substitution in the construction sector is justified and shows great opportunities in its implementation and development.
Ontology-Based Semantic Conceptualisation of Historical Built Heritage to Generate Parametric Structured Models from Point Clouds
Authors: Colucci Elisabetta , Xing Xufeng , Kokla Margarita , Mostafavi Mir Abolfazl , Noardo Francesca , Spanò Antonia
Published: March 2021

Nowadays, cultural and historical built heritage can be more effectively preserved, valorised and documented using advanced geospatial technologies. In such a context, there is a major issue concerning the automation of the process and the extraction of useful information from a huge amount of spatial information acquired by means of advanced survey techniques (i.e., highly detailed LiDAR point clouds). In particular, in the case of historical built heritage (HBH) there are very few effective efforts. Therefore, in this paper, the focus is on establishing the connections between semantic and geometrical information in order to generate a parametric, structured model from point clouds using ontology as an effective approach for the formal conceptualisation of application domains. Hence, in this paper, an ontological schema is proposed to structure HBH representations, starting with international standards, vocabularies, and ontologies (CityGML-Geography Markup Language, International Committee for Documentation conceptual reference model (CIDOC-CRM), Industry Foundation Classes (IFC), Getty Art and Architecture Thesaurus (AAT), as well as reasoning about morphology of historical centres by analysis of real case studies) to represent the built and architecture domain. The validation of such schema is carried out by means of its use to guide the segmentation of a LiDAR point cloud from a castle, which is later used to generate parametric geometries to be used in a historical building information model (HBIM).
The Importance of Annual Plants and Multi-Scalar Analysis for Understanding Coastal Dune Stabilization Process in the Mediterranean
Authors: Bar Kutiel Pua , Dorman Michael
Published: March 2021

Since ecological phenomena and patterns vary with scale, scalar analysis is a developing practice in ecology. Scalar analysis is most valuable in heterogeneous environments, since habitat heterogeneity is a key factor in determining biodiversity. One such case can be seen in the changes in annual vegetation in coastal sand dune systems. Most studies in these environments are carried out at the dune scale, comparing dunes at different stabilization states. However, a broader understanding of dune stabilization processes requires analyses at the finer scales of dune slope aspects (directions of exposure to wind) and patches (under and between woody perennial species). Here, we present the results of a study that combines the three scales (dune, slope, and patch) in the Mediterranean coastal dune systems in Israel. Through this multi-scalar analysis, we are able to describe processes at the finer patch and aspect scale and explain how they shape patterns at the dune scale. The results indicate that the dune scale exposes the differences in annual plant characteristics between mobile and fixed dunes, their slopes and patches and the reorganization and spatial distribution of annual plants within mobile and fixed dunes during the stabilization process.
An Assessment of Indoor Air Quality in the Arrivals Hall of Beirut–Rafic Hariri International Airport: Monitoring of VOCs and NO2
Authors: Mokalled Tharwat , Adjizian Gérard Jocelyne , Abboud Maher , Liaud Céline , Nasreddine Rouba , Le Calvé Stéphane
Published: March 2021

In Beirut–Rafic Hariri International Airport (RHIA), airport employees stay at least 12 h inside the airport’s buildings and suffer from respiratory symptoms. Additionally, direct openings exist between the apron and the arrivals hall providing a pathway for contaminated air to enter the buildings. Hence, we study the impact of Beirut–RHIA’s activities on the indoor air of the arrivals hall (impact on employees and passengers) during June, November, and October 2014. Due to their impacts on air quality and human health, assessing of the concentrations of nitrogen dioxide (NO2) and Volatile Organic Compounds (VOCs) was the target of our study by using gas chromatographic techniques (GC-MS and GC-FID) for VOCs and calorimetric methods for NO2 concentrations. NO2 levels indicated a probable hazard to the health of passengers and employees, while measured VOC levels did not present any risks except for acrolein. This is the first study to assess the speciation of a large number of VOCs (46 VOCs) for airport indoor air while revealing a very interesting correlation between aircraft number and the concentrations of VOC groups (namely heavy alkanes, aldehydes and ketones, and monoaromatics). Moreover, this is the first study in Lebanon to assess the speciation of a large number of VOCs in indoor air.
High Energy Parametric Laser Source and Frequency-Comb-Based Wavelength Reference for CO2 and Water Vapor DIAL in the 2 µm Region: Design and Pre-Development Experimentations
Authors: Hamperl Jonas , Geus Jan Fabian , Mølster Kjell M. , Zukauskas Andrius , Dherbecourt Jean-Baptiste , Pasiskevicius Valdas , Nagy Lukas , Pitz Oliver , Fehrenbacher David , Schaefer Hanjo , Heinecke Dirk , Strotkamp Michael , Rapp Stephan , Denk Paul , Graf Norbert , Dalin Marine , Lebat Vincent , Santagata Rosa , Melkonian Jean-Michel , Godard Antoine , Raybaut Myriam , Flamant Cyrille
Published: March 2021

We present a differential absorption lidar (DIAL) laser transmitter concept designed around a Nested Cavity Optical Parametric Oscillator (NesCOPO) based Master Oscillator Power Amplifier (MOPA). The spectral bands are located around 2051 nm for CO2 probing and 1982 nm for H216O and HD16O water vapor isotopes. This laser is aimed at being integrated into an airborne lidar, intended to demonstrate future spaceborne instrument characteristics: high-energy (several tens of mJ nanosecond pulses) and high optical frequency stability (less than a few hundreds of kHz long term drift). For integration and efficiency purposes, the proposed design is oriented toward the use of state-of-the-art high aperture periodically poled nonlinear materials. This approach is supported by numerical calculations and preliminary experimental validations, showing that it is possible to achieve energies in the 40–50 mJ range, reaching the requirement levels for spaceborne Integrated Path Differential Absorption (IPDA) measurements. We also propose a frequency referencing technique based on beat note measurement of the laser signal with a self-stabilized optical frequency comb, which is expected to enable frequency measurement precisions better than a few 100 kHz over tens of seconds integration time, and will then be used to feed the cavity locking of the NesCOPO.
Silymarin-Enriched Biostimulant Foliar Application Minimizes the Toxicity of Cadmium in Maize by Suppressing Oxidative Stress and Elevating Antioxidant Gene Expression
Authors: Alharby Hesham F. , Al-Zahrani Hassan S. , Hakeem Khalid R. , Alsamadany Hameed , Desoky El-Sayed M. , Rady Mostafa M.
Published: March 2021

For maize, the potential preventive role of foliar spraying with an extract derived from maize grain (MEg, 2%), silymarin (Sm, 0.5 mM), or silymarin-enriched MEg (MEg-Sm) in attenuating the stress effects of cadmium (Cd, 0.5 mM) was examined using a completely randomized design layout. Under normal conditions, foliar spraying with MEg, Sm, or MEg-Sm was beneficial (with MEg-Sm preferred) for maize plants, whereas the benefit was more pronounced under Cd stress. The use of Cd through irrigation water decreased plant growth traits, photosynthetic efficiency, including instantaneous carboxylation efficiency, Fv/Fm, and pigment contents, and hormonal contents (e.g., auxin, gibberellins, cytokinins including trans-zeatin, and salicylic acid). These undesired findings were due to an increase in Cd content, leading to increased levels of oxidative stress (O2•− and H2O2), ionic leakage, and lipid peroxidation. Therefore, this damage resulted in an increase in the activities of nonenzymatic antioxidants, Sm, antioxidative enzymes, and enzyme gene expression. However, under Cd stress, although foliar spray with MEg or Sm had better findings than control, MEg-Sm had better findings than MEg or Sm. Application of MEg-Sm greatly increased photosynthesis efficiency, restored hormonal homeostasis, and further increased the activities of various antioxidants, Sm, antioxidative enzymes, and enzyme gene expression. These desired findings were due to the suppression of the Cd content, and thus the levels of O2•−, H2O2, ionic leakage, and lipid peroxidation, which were positively reflected in the growth and accumulation of dry matter in maize plants. The data obtained in this study recommend applying silymarin-enriched maize grain extract (MEg-Sm at 0.24 g Sm L−1 of MEg) as a spray solution to maize plants when exposed to excess Cd in soil or irrigation water.
Biophysica—A New International Open Access Journal to Integrate Across the Modern Biophysical Sciences and Engineering
Authors: Buck Matthias , Muñoz Victor
Published: March 2021

“What do Biophysicists talk about [...]
Parental Education and Left Lateral Orbitofrontal Cortical Activity during N-Back Task: An fMRI Study of American Adolescents
Authors: Assari Shervin , Boyce Shanika , Saqib Mohammed , Bazargan Mohsen , Caldwell Cleopatra H.
Published: March 2021

Introduction. The Orbitofrontal Cortex (OFC) is a cortical structure that has implications in cognition, memory, reward anticipation, outcome evaluation, decision making, and learning. As such, OFC activity correlates with these cognitive brain abilities. Despite research suggesting race and socioeconomic status (SES) indicators such as parental education may be associated with OFC activity, limited knowledge exists on multiplicative effects of race and parental education on OFC activity and associated cognitive ability. Purpose. Using functional brain imaging data from the Adolescent Brain Cognitive Development (ABCD) study, we tested the multiplicative effects of race and parental education on left lateral OFC activity during an N-Back task. In our study, we used a sociological rather than biological theory that conceptualizes race and SES as proxies of access to the opportunity structure and exposure to social adversities rather than innate and non-modifiable brain differences. We explored racial variation in the effect of parental educational attainment, a primary indicator of SES, on left lateral OFC activity during an N-Back task between Black and White 9–10 years old adolescents. Methods. The ABCD study is a national, landmark, multi-center brain imaging investigation of American adolescents. The total sample was 4290 9–10 years old Black or White adolescents. The independent variables were SES indicators, namely family income, parental education, and neighborhood income. The primary outcome was the average beta weight for N-Back (2 back versus 0 back contrast) in ASEG ROI left OFC activity, measured by functional Magnetic Resonance Imaging (fMRI) during an N-Back task. Ethnicity, age, sex, subjective SES, and family structure were the study covariates. For data analysis, we used linear regression models. Results. In White but not Black adolescents, parental education was associated with higher left lateral OFC activity during the N-Back task. In the pooled sample, we found a significant interaction between race and parental education on the outcome, suggesting that high parental education is associated with a larger increase in left OFC activity of White than Black adolescents. Conclusions. For American adolescents, race and SES jointly influence left lateral OFC activity correlated with cognition, memory, decision making, and learning. Given the central role of left lateral OFC activity in learning and memory, our finding calls for additional research on contextual factors that reduce the gain of SES for Black adolescents. Cognitive inequalities are not merely due to the additive effects of race and SES but also its multiplicative effects.
Design Science and Co-Designing of Hybrid Workplaces
Authors: Lahti Marko , Nenonen Suvi
Published: March 2021

Background: Future places for learning and working are digitally and physically integrated hybrid environments. The archetypical context of learning is the classroom, and context of working is the office; especially in knowledge work. New information and communication technologies enable the spatial reconfiguration of work opening possibilities for work to take place across multiple locations. This paper aims to explore how the conceptual framework of design-science research in Information Systems can be applied when the design object is a hybrid working environment. Methods: The case study method as a qualitative approach was chosen; because it involves an empirical investigation of a particular contemporary phenomenon within its real life context using multiple sources of evidence. The empirical analysis of two hybrid working environments is based on Action Design Research (ADR)-entry points; where one analyzes two case studies stage by stage. By analyzing various stages in both case studies; one can identify co-designing challenges of hybrid working environments. Results: The results present four recommendations for co-designing of hybrid working environments. The use of hybrid working environment; the design of spatial solution; the identification of iterative processes; and the user experiences of presence and distance are significant. The Entry Point Analysis-tool can be used and further developed in analyzing and developing hybrid working environments. Conclusion: The results contribute to the tradition of usability studies. The usability briefing approach can be further developed by identifying the iterative processes inside the linear project management models. Additionally, design science research can find new insights from identification of the large stakeholder iterations more precisely.
Development and Validation of Building Control Algorithm Energy Management
Authors: Han Yerim , Kim Woohyun
Published: March 2021

In this paper, a building control algorithm is proposed to reduce the electricity consumption of a building with a variable refrigerant flow (VRF) system. The algorithm uses sequence-to-sequence long short-term memory (seq2seq LSTM) to set target electricity consumption, and uses a VRF air conditioner system to reduce electricity consumption. After setting target electricity consumption, the algorithm is applied as a method of updating target electricity consumption. In addition, we propose two methods to increase the performance of the seq2seq LSTM model. First, among the feature selection methods, random forest is used to select, among the numerous features of the data, only those features that are most relevant to the predicted value. Second, we use Bayesian optimization, which selects the optimal hyperparameter that shows the best model performance. In order to control the air conditioners, the priority of air conditioners is designated, the method of prioritization being the analytical hierarchy process (AHP). In this study, comparison of the performance of seq2seq LSTM model with and without Bayesian optimization proved that the use of Bayesian optimization achieved good performance. Simulation and demonstration experiments using the algorithm were also conducted, and showed that building electricity consumption decreased in a similar manner to the reduction rate by means of the algorithm.
Genomic, Transcriptomic, and Functional Alterations in DNA Damage Response Pathways as Putative Biomarkers of Chemotherapy Response in Ovarian Cancer
Authors: Sharma Saha Sweta , Gentles Lucy , Bradbury Alice , Brecht Dominik , Robinson Rebecca , O’Donnell Rachel , Curtin Nicola J. , Drew Yvette
Published: March 2021

Defective DNA damage response (DDR) pathways are enabling characteristics of cancers that not only can be exploited to specifically target cancer cells but also can predict chemotherapy response. Defective Homologous Recombination Repair (HRR) function, e.g., due to BRCA1/2 loss, is a determinant of response to platinum agents and PARP inhibitors in ovarian cancers. Most chemotherapies function by either inducing DNA damage or impacting on its repair but are generally used in the clinic unselectively. The significance of HRR and other DDR pathways in determining response to several other chemotherapy drugs is not well understood. In this study, the genomic, transcriptomic and functional analysis of DDR pathways in a panel of 14 ovarian cancer cell lines identified that defects in DDR pathways could determine response to several chemotherapy drugs. Carboplatin, rucaparib, and topotecan sensitivity were associated with functional loss of HRR (validated in 10 patient-derived primary cultures) and mismatch repair. Two DDR gene expression clusters correlating with treatment response were identified, with PARP10 identified as a novel marker of platinum response, which was confirmed in The Cancer Genome Atlas (TCGA) ovarian cancer cohort. Reduced non-homologous end-joining function correlated with increased sensitivity to doxorubicin, while cells with high intrinsic oxidative stress showed sensitivity to gemcitabine. In this era of personalised medicine, molecular/functional characterisation of DDR pathways could guide chemotherapy choices in the clinic allowing specific targeting of ovarian cancers.
Role of bFGF in Acquired Resistance upon Anti-VEGF Therapy in Cancer
Authors: Zahra Fatema Tuz , Sajib Md. Sanaullah , Mikelis Constantinos M.
Published: March 2021

Anti-angiogenic approaches targeting the vascular endothelial growth factor (VEGF) signaling pathway have been a significant research focus during the past decades and are well established in clinical practice. Despite the expectations, their benefit is ephemeral in several diseases, including specific cancers. One of the most prominent side effects of the current, VEGF-based, anti-angiogenic treatments remains the development of resistance, mostly due to the upregulation and compensatory mechanisms of other growth factors, with the basic fibroblast growth factor (bFGF) being at the top of the list. Over the past decade, several anti-angiogenic approaches targeting simultaneously different growth factors and their signaling pathways have been developed and some have reached the clinical practice. In the present review, we summarize the knowledge regarding resistance mechanisms upon anti-angiogenic treatment, mainly focusing on bFGF. We discuss its role in acquired resistance upon prolonged anti-angiogenic treatment in different tumor settings, outline the reported resistance mechanisms leading to bFGF upregulation, and summarize the efforts and outcome of combined anti-angiogenic approaches to date.
Redirecting the Immune Microenvironment in Acute Myeloid Leukemia
Authors: Sendker Stephanie , Reinhardt Dirk , Niktoreh Naghmeh
Published: March 2021

Acute myeloid leukemia is a life-threatening malignant disorder arising in a complex and dysregulated microenvironment that, in part, promotes the leukemogenesis. Treatment of relapsed and refractory AML, despite the current overall success rates in management of pediatric AML, remains a challenge with limited options considering the heavy but unsuccessful pretreatments in these patients. For relapsed/refractory (R/R) patients, hematopoietic stem cell transplantation (HSCT) following ablative chemotherapy presents the only opportunity to cure AML. Even though in some cases immune-mediated graft-versus-leukemia (GvL) effect has been proven to efficiently eradicate leukemic blasts, the immune- and chemotherapy-related toxicities and adverse effects considerably restrict the feasibility and therapeutic power. Thus, immunotherapy presents a potent tool against acute leukemia but needs to be engineered to function more specifically and with decreased toxicity. To identify innovative immunotherapeutic approaches, sound knowledge concerning immune-evasive strategies of AML blasts and the clinical impact of an immune-privileged microenvironment is indispensable. Based on our knowledge to date, several promising immunotherapies are under clinical evaluation and further innovative approaches are on their way. In this review, we first focus on immunological dysregulations contributing to leukemogenesis and progression in AML. Second, we highlight the most promising therapeutic targets for redirecting the leukemic immunosuppressive microenvironment into a highly immunogenic environment again capable of anti-leukemic immune surveillance.
Neutrophil-to-Lymphocyte Ratio Is a Predictive Biomarker in Patients with Epidermal Growth Factor Receptor (EGFR) Mutated Advanced Non-Small Cell Lung Cancer (NSCLC) Treated with Tyrosine Kinase Inhibitor (TKI) Therapy
Authors: Yun Nicole K. , Rouhani Sherin J. , Bestvina Christine M. , Ritz Ethan M. , Gilmore Brendan A. , Tarhoni Imad , Borgia Jeffrey A. , Batus Marta , Bonomi Philip D. , Fidler Mary Jo
Published: March 2021

Background: First-line treatment for patients with non-small cell lung cancer (NSCLC) with a sensitizing epidermal growth factor receptor (EGFR) mutation is a tyrosine kinase inhibitor (TKI). Despite higher response rates and prolonged progression free survival (PFS) compared with platinum doublet chemotherapy, a subset of these patients do not receive prolonged benefit from these agents. We investigate if the neutrophil-to-lymphocyte ratio (NLR) and other markers of cachexia and chronic inflammation correlate with worse outcomes in these patients. Methods: This study is a retrospective review of 137 patients with advanced EGFR-mutated NSCLC treated with TKIs at Rush University Medical Center and University of Chicago Medicine from August 2011 to July 2019, with outcomes followed through July 2020. The predictive value of NLR and body mass index (BMI) was assessed at the start of therapy, and after 6 and 12 weeks of treatment by univariable and multivariable analyses. Results: On univariable analysis, NLR ≥ 5 or higher NLR on a continuous scale were both associated with significantly worse PFS and overall survival (OS) at treatment initiation, and after 6 or 12 weeks of treatment. On multivariable analysis, NLR ≥ 5 was associated with increased risk of death at 12 weeks of therapy (HR 3.002, 95% CI 1.282–7.029, p = 0.011), as was higher NLR on a continuous scale (HR 1.231, 95% CI 1.063–1.425, p = 0.0054). There was no difference in PFS and OS and amongst BMI categories though number of disease sites and Eastern Cooperative Oncology Group (ECOG) performance status was associated with worse PFS and OS. Conclusions: Patients with NLR ≥ 5 have a worse median PFS and median OS than patients with NLR < 5. NLR may have value as a predictive biomarker and may be useful for selecting patients for therapy intensification in the front-line setting either at diagnosis or after 12 weeks on therapy. NLR needs to be validated prospectively.
Epigenetic Mechanisms Are Involved in the Oncogenic Properties of ZNF518B in Colorectal Cancer
Authors: Gimeno-Valiente Francisco , Riffo-Campos Ángela L. , Torres Luis , Tarazona Noelia , Gambardella Valentina , Cervantes Andrés , López-Rodas Gerardo , Franco Luis , Castillo Josefa
Published: March 2021

The ZNF518B gene, which is up-regulated in colorectal cancer, plays a role in cell dissemination and metastasis. It encodes a zinc-finger protein, which interacts with histone methyltransferases G9A and EZH2. The expression of the two major mRNA isoforms 1 (coding for the full protein) and 2 was quantified by RT-qPCR in a cohort of 66 patients. The effects of silencing ZNF518B on the transcriptome of DLD1 and HCT116 cells were analysed by Clariom-S assays and validated by RT-qPCR. The recruitment of methyltransferases and the presence of H3K27me3 were studied by chromatin immunoprecipitation (ChIP). The ratio (isoform 2)/(isoform 1) negatively correlated with the relapsing of disease. The study of the transcriptome of DLD1 and HCT116 cells revealed that many genes affected by silencing ZNF518B are related to cancer. After crossing these results with the list of genes affected by silencing the histone methyltransferases (retrieved in silico), five genes were selected. ChIP analysis revealed that the recruitment of EZH2 is ZNF518B-dependent in KAT2B, RGS4 and EFNA5; the level of H3K27me3 changes in accordance. G9A also binds RGS4 and PADI3 in a ZNF518B-dependent manner. The results highlight the importance of epigenetics in cancer and open a novel therapeutic possibility, as inhibition of histone methyltransferases may reverse the disease-linked histone marks.
Prediction of Unplanned Hospitalizations in Older Patients Treated with Chemotherapy
Authors: Feliu Jaime , Espinosa Enrique , Basterretxea Laura , Paredero Irene , Llabrés Elisenda , Jiménez-Munárriz Beatriz , Losada Beatriz , Pinto Alvaro , Custodio Ana Belén , Muñoz María del Mar , Gómez-Mediavilla Jeniffer , Torregrosa María Dolores , Cruz Patricia , Higuera Oliver , Molina-Garrido María José
Published: March 2021

Purpose: To determine the incidence of unplanned hospitalization (UH) and to identify risk factors for UH in elderly patients with cancer who start chemotherapy. Methods: In all, 493 patients over 70 years starting new chemotherapy regimens were prospectively included. A pre-chemotherapy geriatric assessment was performed, and tumor and treatment variables were collected. The association between these factors and UH was examined by using multivariable logistic regression. Score points were assigned to each risk factor. Results: During the first 6 months of treatment, 37% of patients had at least one episode of UH. Risk factors were the use of combination chemotherapy at standard doses, a MAX2 index ≥1, a Charlson comorbidity score ≥2, albumin level <3.5 g/dL, falls in the past 6 months ≥1, and weight loss >5%. Three risk groups for UH were established according to the score in all patients: 0–1: 17.5%; 2: 34%; and 3–7: 57% (p < 0.001). The area under receiver operation characteristic (ROC) curve was 0.72 (95% CI: 0.67–0.77). Conclusion: This simple tool can help to reduce the incidence of UH in elderly patients with cancer who are scheduled to initiate chemotherapy treatment.
Deacetylase Plus Bromodomain Inhibition Downregulates ERCC2 and Suppresses the Growth of Metastatic Colon Cancer Cells
Authors: Kapoor Sabeeta , Gustafson Trace , Zhang Mutian , Chen Ying-Shiuan , Li Jia , Nguyen Nhung , Perez Jorge Enrique Tovar , Dashwood Wan Mohaiza , Rajendran Praveen , Dashwood Roderick H.
Published: March 2021

There is growing evidence that DNA repair factors have clinical value for cancer treatment. Nucleotide excision repair (NER) proteins, including excision repair cross-complementation group 2 (ERCC2), play a critical role in maintaining genome integrity. Here, we examined ERCC2 expression following epigenetic combination drug treatment. Attention was drawn to ERCC2 for three reasons. First, from online databases, colorectal cancer (CRC) patients exhibited significantly reduced survival when ERCC2 was overexpressed in colon tumors. Second, ERCC2 was the most highly downregulated RNA transcript in human colon cancer cells, plus Ercc2 in rat tumors, after treatment with the histone deacetylase 3 (HDAC3) inhibitor sulforaphane (SFN) plus JQ1, which is an inhibitor of the bromodomain and extraterminal domain (BET) family. Third, as reported here, RNA-sequencing of polyposis in rat colon (Pirc) polyps following treatment of rats with JQ1 plus 6-methylsulfinylhexyl isothiocyanate (6-SFN) identified Ercc2 as the most highly downregulated gene. The current work also defined promising second-generation epigenetic drug combinations with enhanced synergy and efficacy, especially in metastasis-lineage colon cancer cells cultured as 3D spheroids and xenografts. This investigation adds to the growing interest in combination approaches that target epigenetic ‘readers’, ‘writers’, and ‘erasers’ that are deregulated in cancer and other pathologies, providing new avenues for precision oncology and cancer interception.
How Does Endometriosis Lead to Ovarian Cancer? The Molecular Mechanism of Endometriosis-Associated Ovarian Cancer Development
Authors: Yachida Nozomi , Yoshihara Kosuke , Yamaguchi Manako , Suda Kazuaki , Tamura Ryo , Enomoto Takayuki
Published: March 2021

Numerous epidemiological and histopathological studies support the notion that clear cell and endometrioid carcinomas derive from ovarian endometriosis. Accordingly, these histologic types are referred to as “endometriosis-associated ovarian cancer” (EAOC). Although the uterine endometrium is also considered an origin of endometriosis, the molecular mechanism involved in transformation of the uterine endometrium to EAOC via ovarian endometriosis has not yet been clarified. Recent studies based on high-throughput sequencing technology have revealed that cancer-associated gene mutations frequently identified in EAOC may exist in the normal uterine endometrial epithelium and ovarian endometriotic epithelium. The continuum of genomic alterations from the uterine endometrium to endometriosis and EAOC has been described, though the significance of cancer-associated gene mutations in the uterine endometrium or endometriosis remains unclear. In this review, we summarize current knowledge regarding the molecular characteristics of the uterine endometrium, endometriosis, and EAOC and discuss the molecular mechanism of cancer development from the normal endometrium through endometriosis in an effort to prevent EAOC.