bluuebunny/crossref_metadata_2025 huggingface dataset:
Trends of Surgical Service Utilization for Lumbar Spinal Stenosis in South Korea: A 10-Year (2010–2019) Cross-Sectional Analysis of the Health Insurance Review and Assessment Service—National Patient Sample Data
Authors: Ji HyungWook , Shin Seungwon , Kim Yongjoo , Ha In-Hyuk , Kim Doori , Lee Yoon Jae
Published: August 2023

Background and Objectives: This retrospective, cross-sectional, and descriptive study used claims data from the Korean Health Insurance Review and Assessment Service (HIRA) between 2010 and 2019 to analyze the trend of surgical service utilization in patients with lumbar spinal stenosis (LSS). Materials and Methods: The national patient sample data provided by the HIRA, which consisted of a 2% sample of the entire Korean population, was used to assess all patients who underwent decompression or fusion surgery at least once in Korea, with LSS as the main diagnosis from January 2010 to December 2019. An in-depth analysis was conducted to examine the utilization of surgical services, taking into account various demographic characteristics of patients, the frequency of claims for different types of surgeries, reoperation rates, the specific types of inpatient care associated with each surgery type, prescribed medications, and the overall expense of healthcare services. Results: A total of 6194 claims and 6074 patients were analyzed. The number of HIRA claims for patients increased from 393 (2010) to 417 (2019) for decompression, and from 230 (2010) to 244 (2019) for fusion. As for the medical expenses of surgery, there was an increase from United States dollar (USD) 867,549.31 (2010) to USD 1,153,078.94 (2019) for decompression and from USD 1,330,440.37 (2010) to USD 1,780,026.48 (2019) for fusion. Decompression accounted for the highest proportion (65.8%) of the first surgeries, but more patients underwent fusion (50.6%) than decompression (49.4%) in the second surgery. Across all sex and age groups, patients who underwent fusion procedures experienced longer hospital stays and incurred higher medical expenses for their inpatient care. Conclusion: The surgical service utilization of patients with LSS and the prescribing rate of opioids and non-opioid analgesics for surgical patients increased in 2019 compared to 2010. From mid-2010 onward, claims for fusion showed a gradual decrease, whereas those for decompression showed a continuously increasing trend. The findings of this study are expected to provide basic research data for clinicians, researchers, and policymakers.
Effects of Building Directions on Microstructure, Impurity Elements and Mechanical Properties of NiTi Alloys Fabricated by Laser Powder Bed Fusion
Authors: Wang Shuo , Yang Xiao , Chen Jieming , Pan Hengpei , Zhang Xiaolong , Zhang Congyi , Li Chunhui , Liu Pan , Zhang Xinyao , Gao Lingqing , Wang Zhenzhong
Published: August 2023

For NiTi alloys prepared by the Laser Powder Bed Fusion (LPBF), changes in the building directions will directly change the preferred orientation and thus directly affect the smart properties, such as superelasticity, as well as change the distribution state of defects and impurity elements to affect the phase transformation behaviour, which in turn affects the smart properties at different temperatures. In this study, the relationship between impurity elements, the building directions, and functional properties; the effects of building directions on the crystallographic anisotropy; phase composition; superelastic properties; microhardness; geometrically necessary dislocation (GND) density; and impurity element content of NiTi SMAs fabricated by LPBF were systematically studied. Three building directions measured from the substrate, namely, 0°, 45° and 90°, were selected, and three sets of cylindrical samples were fabricated with the same process parameters. Along the building direction, a strong <100>//vertical direction (VD) texture was formed for all the samples. Because of the difference in transformation temperature, when tested at 15 °C, the sample with the 45° orientation possessed the highest strain recovery of 3.2%. When tested at the austenite phase transformation finish temperature (Af)+10 °C, the 90° sample had the highest strain recovery of 5.83% and a strain recovery rate of 83.3%. The sample with the 90° orientation presented the highest microhardness, which was attributed to its high dislocation density. Meanwhile, different building directions had an effect on the contents of O, C, and N impurity elements, which affected the transformation temperature by changing the Ni/Ti ratio. This study innovatively studied the impurity element content and GND densities of compressive samples with three building directions, providing theoretical guidance for LPBFed NiTi SMA structural parts.
Ecological Effect of Differently Treated Wooden Materials on Microalgal Biofilm Formation in the Grado Lagoon (Northern Adriatic Sea)
Authors: Natali Vanessa , Malfatti Francesca , Cibic Tamara
Published: August 2023

Within the framework of the Interreg Italy–Slovenia programme, the project DuraSoft aimed at testing innovative technologies to improve the durability of traditional wooden structures in socio-ecologically sensitive environments. We focused on the impact of different wood treatments (i.e., copper-based coatings and thermal modification) on microbial biofilm formation in the Grado Lagoon. Wooden samples were placed in 2 areas with diverse hydrodynamic conditions and retrieved after 6, 20, and 40 days. Light, confocal and scanning electron microscopy were employed to assess the treatment effects on the microalgal community abundance and composition. Lower hydrodynamics accelerated the colonisation, leading to higher algal biofilm abundances, regardless of the treatment. The Cu-based agents induced modifications to the microalgal community, leading to lower densities, small-sized diatoms and frequent deformities (e.g., bent apices, frustule malformation) in the genera Cylindrotheca and Cocconeis. After 20 days, taxa forming 3D mucilaginous structures, such as Licmophora and Synedra, were present on chemically treated panels compared to natural ones. While in the short term, the treatments were effective as antifouling agents, in the long term, neither the copper-based coatings nor the thermal modification successfully slowed down the biofouling colonisation, likely due to the stimulating effect of nutrients and other substances released from these solutions. The need to develop more ecosystem friendly technologies to preserve wooden structures remains urgent.
Engineering the Metabolic Landscape of Microorganisms for Lignocellulosic Conversion
Authors: Peña-Castro Julián Mario , Muñoz-Páez Karla M. , Robledo-Narvaez Paula N. , Vázquez-Núñez Edgar
Published: August 2023

Bacteria and yeast are being intensively used to produce biofuels and high-added-value products by using plant biomass derivatives as substrates. The number of microorganisms available for industrial processes is increasing thanks to biotechnological improvements to enhance their productivity and yield through microbial metabolic engineering and laboratory evolution. This is allowing the traditional industrial processes for biofuel production, which included multiple steps, to be improved through the consolidation of single-step processes, reducing the time of the global process, and increasing the yield and operational conditions in terms of the desired products. Engineered microorganisms are now capable of using feedstocks that they were unable to process before their modification, opening broader possibilities for establishing new markets in places where biomass is available. This review discusses metabolic engineering approaches that have been used to improve the microbial processing of biomass to convert the plant feedstock into fuels. Metabolically engineered microorganisms (MEMs) such as bacteria, yeasts, and microalgae are described, highlighting their performance and the biotechnological tools that were used to modify them. Finally, some examples of patents related to the MEMs are mentioned in order to contextualize their current industrial use.
Macrophage Activation in Follicular Conjunctivitis during the COVID-19 Pandemic
Authors: Gallenga Carla Enrica , Maritati Martina , Mura Marco , Di Virgilio Francesco , Conti Pio , Contini Carlo
Published: August 2023

Among the symptoms of SARS-CoV-2, follicular conjunctivitis has become relevant. The conjunctiva acts as an open lymph node, reacting to the viral antigen that binds the epithelial cells, forming follicles of B cells with activated T cells and NK cells on its surface, which, in turn, talk to monocyte-derived inflammatory infected macrophages. Here, the NLRP3 inflammasome is a major driver in releasing pro-inflammatory factors such as IL-6 and caspase-1, leading to follicular conjunctivitis and bulbar congestion, even as isolated signs in the ‘asymptomatic’ patient.
Evaluation of Different Activity of Lactobacillus spp. against Two Proteus mirabilis Isolated Clinical Strains in Different Anatomical Sites In Vitro: An Explorative Study to Improve the Therapeutic Approach
Authors: Fusco Alessandra , Savio Vittoria , Chiaromonte Adriana , Alfano Alberto , D’Ambrosio Sergio , Cimini Donatella , Donnarumma Giovanna
Published: August 2023

Urinary tract infections (UTIs) and catheter-associated UTIs (CAUTIs) are the principal hospital-acquired infections. Between these, bacterial prostatitis is believed to be the leading cause of recurrent UTIs in men under 50 years of age and is often unresponsive to antibiotic treatment. Proteus mirabilis is more commonly associated with UTIs in these abnormalities, especially in patients undergoing catheterization. Lactobacillus spp. are an important component of the human microbiota and occur in large quantities in foods. Probiotics are proposed as an alternative to antibiotic therapy in the treatment of urinary tract infections. In addition to their ability to produce antimicrobial metabolites, they have immunomodulatory activity and do not cause side effects. For this reason, the combination of probiotic microorganisms and conventional drugs was considered. The aim of this work was to select the most active Lactobacillus strains against two clinical isolates of P. mirabilis on bladder and prostatic epithelium, potentially exploitable to improve the clinical management of UTIs.
Mechanisms of Lung and Intestinal Microbiota and Innate Immune Changes Caused by Pathogenic Enterococcus Faecalis Promoting the Development of Pediatric Pneumonia
Authors: Tian Zhiying , Deng Ting , Gui Xuwen , Wang Leilei , Yan Qiulong , Wang Liang
Published: August 2023

Bacterial pneumonia is the main cause of illness and death in children under 5 years old. We isolated and cultured pathogenic bacteria LE from the intestines of children with pneumonia and replicated the pediatric pneumonia model using an oral gavage bacterial animal model. Interestingly, based on 16srRNA sequencing, we found that the gut and lung microbiota showed the same imbalance trend, which weakened the natural resistance of this area. Further exploration of its mechanism revealed that the disruption of the intestinal mechanical barrier led to the activation of inflammatory factors IL-6 and IL-17, which promoted the recruitment of ILC-3 and the release of IL-17 and IL-22, leading to lung inflammation. The focus of this study is on the premise that the gut and lung microbiota exhibit similar destructive changes, mediating the innate immune response to promote the occurrence of pneumonia and providing a basis for the development and treatment of new drugs for pediatric pneumonia.
LPS-Induced Mortality in Zebrafish: Preliminary Characterisation of Common Fish Pathogens
Authors: Santos Rafaela A. , Cardoso Cláudia , Pedrosa Neide , Gonçalves Gabriela , Matinha-Cardoso Jorge , Coutinho Filipe , Carvalho António P. , Tamagnini Paula , Oliva-Teles Aires , Oliveira Paulo , Serra Cláudia R.
Published: August 2023

Disease outbreaks are a common problem in aquaculture, with serious economic consequences to the sector. Some of the most important bacterial diseases affecting aquaculture are caused by Gram-negative bacteria including Vibrio spp. (vibriosis), Photobacterium damselae (photobacteriosis), Aeromonas spp. (furunculosis; haemorrhagic septicaemia) or Tenacibaculum maritimum (tenacibaculosis). Lipopolysaccharides (LPS) are important components of the outer membrane of Gram-negative bacteria and have been linked to strong immunogenic responses in terrestrial vertebrates, playing a role in disease development. To evaluate LPS effects in fish, we used a hot-phenol procedure to extract LPS from common fish pathogens. A. hydrophila, V. harveyi, T. maritimum and P. damselae purified LPS were tested at different concentrations (50, 100, 250 and 500 µg mL−1) at 3 days post-fertilisation (dpf) Danio rerio larvae, for 5 days. While P. damselae LPS did not cause any mortality under all concentrations tested, A. hydrophila LPS induced 15.5% and V. harveyi LPS induced 58.3% of zebrafish larvae mortality at 500 µg mL−1. LPS from T. maritimum was revealed to be the deadliest, with a zebrafish larvae mortality percentage of 80.6%. Analysis of LPS separated by gel electrophoresis revealed differences in the overall LPS structure between the bacterial species analysed that might be the basis for the different mortalities observed.
A Series of Novel 3D Coordination Polymers Based on the Quinoline-2,4-dicarboxylate Building Block and Lanthanide(III) Ions—Temperature Dependence Investigations
Authors: Vlasyuk Dmytro , Łyszczek Renata , Mazur Liliana , Pladzyk Agnieszka , Hnatejko Zbigniew , Woźny Przemysław
Published: August 2023

A series of novel 3D coordination polymers [Ln2(Qdca)3(H2O)x]·yH2O (x = 3 or 4, y = 0–4) assembled from selected lanthanide ions (Ln(III) = Nd, Eu, Tb, and Er) and a non-explored quinoline-2,4-dicarboxylate building block (Qdca2− = C11H5NO42−) were prepared under hydrothermal conditions at temperatures of 100, 120, and 150 °C. Generally, an increase in synthesis temperature resulted in structural transformations and the formation of more hydrated compounds. The metal complexes were characterized by elemental analysis, single-crystal and powder X-ray diffraction methods, thermal analysis (TG-DSC), ATR/FTIR, UV/Vis, and luminescence spectroscopy. The structural variety of three-dimensional coordination polymers can be ascribed to the temperature effect, which enforces the diversity of quinoline-2,4-dicarboxylate ligand denticity and conformation. The Qdca2− ligand only behaves as a bridging or bridging–chelating building block binding two to five metal centers with seven different coordination modes arising mainly from different carboxylate group coordination types. The presence of water molecules in the structures of complexes is crucial for their stability. The removal of both coordinated and non-coordinated water molecules leads to the disintegration and combustion of metal–organic frameworks to the appropriate lanthanide oxides. The luminescence features of complexes, quantum yield, and luminescent lifetimes were measured and analyzed. Only the Eu complexes show emission in the VIS region, whereas Nd and Er complexes emit in the NIR range. The luminescence properties of complexes were correlated with the crystal structures of the investigated complexes.
Determination of Fluoxetine in Weight Loss Herbal Medicine Using an Electrochemical Sensor Based on rGO-CuNPs
Authors: Melaré Aline Giuli , Barreto Francisco Contini , Silva Martin Kassio Leme , Simões Rafael Plana , Cesarino Ivana
Published: August 2023

The rising popularity of herbal medicine as a weight loss remedy, fueled by misleading propaganda, raises concerns about the manufacturing processes and potential inclusion of controlled substances such as fluoxetine (FLU). The objective of this work is to develop and evaluate the performance of an electrochemical device by modifying a glassy carbon electrode (GC) with a nanocomposite based on reduced graphene oxide (rGO) and copper nanoparticles (CuNPs) for detecting FLU in manipulated herbal medicines. Scanning electron microscopy (FEG-SEM) and cyclic voltammetry (CV) were applied for morphological and electrochemical characterization and analysis of the composite’s electrochemical behavior. Under optimized conditions, the proposed sensor successfully detected FLU within the range of 0.6 to 1.6 µmol L−1, showing a limit of detection (LOD) of 0.14 µmol L−1. To determine the presence of FLU in herbal samples, known amounts of the analytical standard were added to the sample, and the analyses were performed using the standard addition method, yielding recoveries between −2.13 and 2.0%.
Synthesis and Biological Activities of Luminescent 5,6-Membered Bis(Metallacyclic) Platinum(II) Complexes
Authors: Jing Jing , Yu Miao , Pan Lei , Zhao Yang , Xu Guo , Zhang Hua-Hong , Li Chen , Zhang Xiao-Peng
Published: August 2023

Four couples of 5,6-membered bis(metallacyclic) Pt(II) complexes with acetylide and isocyanide auxiliary ligands have been prepared and characterized. The structures of (−)-2 and (−)-3 are confirmed by single-crystal X-ray diffraction, showing a distorted square-planar coordination environment around the Pt(II) nucleus. Both solutions and solid samples of all complexes are emissive at RT. Acetylide-coordinated Pt(II) complexes have a lower energy emission than those isocyanide-coordinated ones. The emission spectra of N^N′*C-coordinated Pt(II) derivatives show a lower energy emission maximum relative to N^C*N′-coordinated complexes with the same auxiliary ligand. Moreover, the difference between cyclometalated N^N′*C and N^C*N′ ligands exerts a more remarkable effect on the emission than the auxiliary ligands acetylide and isocyanide. Cytotoxicity and cell imaging of luminescent 5,6-membered bis(metallacyclic) Pt(II) complexes have been evaluated.
In Silico Modeling and Structural Analysis of Soluble Epoxide Hydrolase Inhibitors for Enhanced Therapeutic Design
Authors: Sar Shuvam , Mitra Soumya , Panda Parthasarathi , Mandal Subhash C. , Ghosh Nilanjan , Halder Amit Kumar , Cordeiro Maria Natalia D. S.
Published: August 2023

Human soluble epoxide hydrolase (sEH), a dual-functioning homodimeric enzyme with hydrolase and phosphatase activities, is known for its pivotal role in the hydrolysis of epoxyeicosatrienoic acids. Inhibitors targeting sEH have shown promising potential in the treatment of various life-threatening diseases. In this study, we employed a range of in silico modeling approaches to investigate a diverse dataset of structurally distinct sEH inhibitors. Our primary aim was to develop predictive and validated models while gaining insights into the structural requirements necessary for achieving higher inhibitory potential. To accomplish this, we initially calculated molecular descriptors using nine different descriptor-calculating tools, coupled with stochastic and non-stochastic feature selection strategies, to identify the most statistically significant linear 2D-QSAR model. The resulting model highlighted the critical roles played by topological characteristics, 2D pharmacophore features, and specific physicochemical properties in enhancing inhibitory potential. In addition to conventional 2D-QSAR modeling, we implemented the Transformer-CNN methodology to develop QSAR models, enabling us to obtain structural interpretations based on the Layer-wise Relevance Propagation (LRP) algorithm. Moreover, a comprehensive 3D-QSAR analysis provided additional insights into the structural requirements of these compounds as potent sEH inhibitors. To validate the findings from the QSAR modeling studies, we performed molecular dynamics (MD) simulations using selected compounds from the dataset. The simulation results offered crucial insights into receptor–ligand interactions, supporting the predictions obtained from the QSAR models. Collectively, our work serves as an essential guideline for the rational design of novel sEH inhibitors with enhanced therapeutic potential. Importantly, all the in silico studies were performed using open-access tools to ensure reproducibility and accessibility.
Unprecedented Neoverrucosane and Cyathane Diterpenoids with Anti-Neuroinflammatory Activity from Cultures of the Culinary-Medicinal Mushroom Hericium erinaceus
Authors: Wei Jing , Li Jia-yao , Feng Xi-long , Zhang Yilin , Hu Xuansheng , Hui Heping , Xue Xiaodong , Qi Jianzhao
Published: August 2023

The culinary medicinal mushroom Hericium erinaceus holds significant global esteem and has garnered heightened interest within increasingly ageing societies due to its pronounced neuroprotective and anti-neuroinflammatory properties. Within this study, two novel diterpenes, 16-carboxy-13-epi-neoverrucosane (1) and Erinacine L (2); three known xylosyl cyathane diterpenoids, Erinacine A (3), Erinacine C (4), and Erinacine F (5); and four lanostane-type triterpenoids, and three cyclic dipeptides (10–12), in addition to orcinol (13), were isolated from the rice-based cultivation medium of H. erinaceus. Their structures were determined by NMR, HR-ESI-MS, ECD, and calculated NMR. Compound 1 marks a pioneering discovery as the first verrucosane diterpene originating from basidiomycetes, amplifying the scope of fungal natural product chemistry, and the intricate stereochemistry of Compound 5 has been comprehensively assessed for the first time. Compounds 2–5 not only showed encouraging neurotrophic activity in rat adrenal pheochromocytoma PC-12 cells, but also significantly inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV2 microglia cell cultures with IC50 values as low as 5.82 ± 0.18 μM. To elucidate the mechanistic underpinnings of these bioactivities, molecular docking simulation was used to analyze and support the interaction of 1 and 2 with inducible NO synthase (iNOS), respectively. In particular, compound 2, a cyathane-xyloside containing an unconventional hemiacetal moiety, is a compelling candidate for the prevention of neurodegenerative diseases. In summation, this investigation contributes substantively to the panorama of fungal diterpene structural diversity, concurrently furnishing additional empirical substantiation for the role of cyathane diterpenes in the amelioration of neurodegenerative afflictions.
Exploring the Effects of Various Two-Dimensional Supporting Materials on the Water Electrolysis of Co-Mo Sulfide/Oxide Heterostructure
Authors: Huynh Ngoc-Diem , Choi Won Mook , Hur Seung Hyun
Published: August 2023

In this study, various two-dimensional (2D) materials were used as supporting materials for the bimetallic Co and Mo sulfide/oxide (CMSO) heterostructure. The water electrolysis activity of CMSO supported on reduced graphene oxide (rGO), graphite carbon nitride (gC3N4), and siloxene (SiSh) was better than that of pristine CMSO. In particular, rGO-supported CMSO (CMSO@rGO) exhibited a large surface area and a low interface charge-transfer resistance, leading to a low overpotential and a Tafel slope of 259 mV (10 mA/cm2) and 85 mV/dec, respectively, with excellent long-term stability over 40 h of continuous operation in the oxygen evolution reaction.
Multi-Charge Storage Layer Model of High-Charge-Density Triboelectric Nanogenerator
Authors: Cui Xin , Zhang Yaming , Zhang Yan
Published: August 2023

Triboelectric nanogenerators (TENGs) are key technologies for the Internet of Things with energy harvesting. To improve energy conversion efficiency and convert mechanical energy into electrical energy, high charge density in TENGs plays a crucial role in the design of triboelectric materials and device structures. This paper proposes mechanisms and strategies to increase TENGs’ charge density through multi-charge storage layers. We also discuss the realization of higher charge densities through material and structure design. The implementation of novel charge storage strategies holds the potential for significant improvements in charge density.
Barriers and Facilitators to Cardiovascular Disease Prevention Following Hypertensive Disorders of Pregnancy in Primary Care: Cross-Sectional Surveys
Authors: Slater Kaylee , Taylor Rachael , McLaughlin Karen , Pennell Craig , Collins Clare , Hutchesson Melinda
Published: August 2023

Women with a history of hypertensive disorders of pregnancy (HDP) have an increased risk of cardiovascular disease (CVD). Guidelines recommend that women diagnosed with HDP should be advised of their increased CVD risk, have regular blood pressure monitoring by their general practitioner (GP), and adopt healthy lifestyle behaviours. However, within Australia, the current practice in primary health care is unknown. The aim of this study was to describe current practices, barriers, and facilitators to the provision of CVD preventative services for women after HDP in the primary care setting and to identify potential strategies to support GPs in providing recommended care. Separate cross-sectional online surveys were undertaken with 35 GPs and 105 women with a history of HDP. Surveys included both closed- and open-ended questions. Closed-ended questions were analysed using basic descriptive statistics, and open-ended questions were themed and tallied. The survey of GPs revealed that GPs are more likely to assess traditional CVD risk markers than lifestyle risk factors or HDP history. GPs identified a lack of resources and skills as barriers to providing CVD preventative care post-HDP. The survey with women after HDP revealed that women with a history of HDP are more likely to be assessed for blood pressure than lifestyle CVD risk factors, and that the women’s barriers to obtaining care included difficulty obtaining an appointment and time required for attending appointments. Strategies to improve CVD preventative care were consistent between surveys, where 70% of GPs and 59% of women chose ‘increasing women’s awareness of increased CVD risk’ and 67% of GPs and 55% of women chose ‘improving communication between hospitals and primary care’ as their preferred strategies. While the findings suggest that women with a history of HDP are receiving advice consistent with guidelines for traditional CVD risk markers, such as blood pressure, they are less likely to receive CVD preventative care for lifestyle or female-specific CVD risk factors.
Effect of Moderate-Intense Training and Detraining on Glucose Metabolism, Lipid Profile, and Liver Enzymes in Male Wistar Rats: A Preclinical Randomized Study
Authors: Shakoor Hira , Kizhakkayil Jaleel , Khalid Mariyam , Mahgoub Amar , Platat Carine
Published: August 2023

Exercise training positively regulates glucose metabolism. This study investigated the impact of training and detraining on glucose metabolism, lipid profiles, and liver enzymes. Twenty-six rats completed an initial 4-week moderate-intense training (T0–T4). Then, the animals were randomly assigned to two groups at the end of week 4: AT4: detraining for 8 weeks; AT8: training for 8 weeks and 4-week detraining. Six animals were sacrificed at T0 and T4, four animals/group at T8, and three/group at T12. The study continued for 12 weeks, and all parameters were assessed at T0, T4, T8, and T12. IPGTT significantly improved after 4 weeks of training (p < 0.01) and was further reduced in AT8 at T8. In AT8, 8-week training significantly reduced total cholesterol at T4 and T12 vs. T0 (p < 0.05), LDL at T4, T8, and T12 vs. T0 (p < 0.01), ALP at T8, T12 vs. T0 (p < 0.01), and increased HDL at T8 and ALT at T8 and T12 vs. T0 (p < 0.05). Triglycerides and hexokinase activity increased significantly at T4 and T8 (p < 0.05) and then decreased at T12 in AT8. Pyruvate and glycogen increased at T12 in AT8 vs. AT4. Eight-week training improved LPL and ATGL expressions. Training positively modulated insulin, glucose metabolism, and lipid profiles, but detraining reduced the benefits associated with the initial training.
Special Issue “Osteosarcomas: Treatment Strategies”
Authors: Park See-Hyoung
Published: August 2023

This Special Issue, titled “Osteosarcomas: Treatment Strategies”, aims to overview the recent and future research trends related to the treatment of osteosarcoma [...]
Fe2O3 Nanoparticle-Based Q-Switched Pulse Fiber Laser
Authors: Muhammad Farah Diana , Chyi Josephine Liew Ying , Mohd Asran Anom Nazirah , Alresheedi Mohammed Thamer , Ng Eng Khoon , Mahdi Mohd Adzir
Published: August 2023

We demonstrate the utilization of iron oxide (Fe2O3) as light-absorbing material in an erbium-doped fiber laser (EDFL) for the generation of Q-switched pulses. A sandwich-type saturable absorber (SA) with Fe2O3 nanoparticles between fiber ferrules is proposed. A fiber ferrule tip is tapped onto a cap of index-matching gel, which is then dipped into Fe2O3 nanoparticle powder to allow its deposition through the adhesion effect. By incorporating Fe2O3–SA in an EDFL, self-started and stable Q-switched pulses are attained at a threshold power of 50.1 mW. The pulse repetition rate is tunable from 9.92 kHz to 22.47 kHz, whereas the pulse duration reduces from 38.4 µs to 13.8 µs with the pump power increment. The maximum pulse energy achieved is 36.9 nJ. This work offers a simple integration method of Fe2O3 nanoparticles as potential SAs for the generation of Q-switched pulses.
Laser Output Performance and Temporal Quality Enhancement at the J-KAREN-P Petawatt Laser Facility
Authors: Kiriyama Hiromitsu , Miyasaka Yasuhiro , Kon Akira , Nishiuchi Mamiko , Sagisaka Akito , Sasao Hajime , Pirozhkov Alexander S. , Fukuda Yuji , Ogura Koichi , Kondo Kotaro , Nakanii Nobuhiko , Mashiba Yuji , Dover Nicholas P. , Chang Liu , Kando Masaki , Bock Stefan , Ziegler Tim , Püschel Thomas , Schlenvoigt Hans-Peter , Zeil Karl , Schramm Ulrich
Published: August 2023

We described the output performance and temporal quality enhancement of the J-KAREN-P petawatt laser facility. After wavefront correction using a deformable mirror, focusing with an f/1.3 off-axis parabolic mirror delivered a peak intensity of 1022 W/cm2 at 0.3 PW power levels. Technologies to improve the temporal contrast were investigated and tested. The origins of pre-pulses generated by post-pulses were identified and the elimination of most pre-pulses by removal of the post-pulses with wedged optics was achieved. A cascaded femtosecond optical parametric amplifier based on the utilization of the idler pulse rather than the signal pulse was developed for the complete elimination of the remaining pre-pulses. The orders of magnitude enhancement of the pedestal before the main pulse were obtained by using a higher surface quality of the convex mirror in the Öffner stretcher. A single plasma mirror was installed in the J-KAREN-P laser beam line for further contrast improvement of three orders of magnitude. The above developments indicate, although it has not been directly measured, the contrast can be as high as approximately 1015 up to 40 ps before the main pulse. We also showed an overview of the digital transformation (DX) of the system, enabling remote and automated operation of the J-KAREN-P laser facility.
Lasers in Medicine: The Changing Role of Therapeutic Laser-Induced Retinal Damage—From de rigeuer to Nevermore
Authors: Luttrull Jeffrey K.
Published: August 2023

For over five decades, laser-induced retinal damage (LIRD) was thought to be the necessary cost of all therapeutic effects of laser treatment for the most important causes of irreversible visual loss, the chronic progressive retinopathies (CPRs). The development of modern retinal laser therapy with the discovery of “low-intensity/high-density subthreshold micropulse” laser (SDM) showed that the supposed need for LIRD represented a case of confusing association with causation. This revealed that LIRD was unnecessary and detrimental to clinical outcomes, and thus, contraindicated as the most severe complication of retinal laser treatment. SDM allowed for an understanding of the mechanism of retinal laser treatment as a physiologic reset effect, triggered by heat-shock protein (HSP) activation upregulating the unfolded protein response and restoring proteostasis by increasing protein repair by 35% in dysfunctional cells via a thermally sensitive conformational change in the K10 step of HSP activation kinetics. Because HSP activation kinetics are catalytic, even low levels (the “reset” threshold) of HSP activation result in a maximal treatment response. SDM and the study of HSP activation kinetics in the retina show that the therapeutic effects of retinal laser treatment can be fully realized without any degree of LIRD. Besides LIRD, all effects of retinal laser treatment are restorative and therapeutic, without any known adverse treatment effects. Without LIRD, the benefits of retinal laser treatment are infinitely renewable and direct treatment of the fovea is possible. Elimination of LIRD from retinal laser treatment has revolutionized the clinical potential of retinal laser treatment to broaden treatment indications to permit, for the first time, effective early and preventive treatment to reduce visual loss from the most frequent causes of irreversible visual loss worldwide, the CPRs.
Reconstructed Global Invasion and Spatio-Temporal Distribution Pattern Dynamics of Sorghum halepense under Climate and Land-Use Change
Authors: Yang Ming , Zhao Haoxiang , Xian Xiaoqing , Qi Yuhan , Li Qiao , Guo Jianying , Chen Li , Liu Wanxue
Published: August 2023

Sorghum halepense competes with crops and grass species in cropland, grassland, and urban environments, increasing invasion risk. However, the invasive historical dynamics and distribution patterns of S. halepense associated with current and future climate change and land-use change (LUC) remain unknown. We first analyzed the invasive historical dynamics of S. halepense to explore its invasion status and expansion trends. We then used a species distribution model to examine how future climate change and LUC will facilitate the invasion of S. halepense. We reconstructed the countries that have historically been invaded by S. halepense based on databases with detailed records of countries and occurrences. We ran biomod2 based on climate data and land-use data at 5′ resolution, assessing the significance of environmental variables and LUC. Sorghum halepense was widely distributed worldwide through grain trade and forage introduction, except in Africa. Europe and North America provided most potential global suitable habitats (PGSHs) for S. halepense in cropland, grassland, and urban environments, representing 48.69%, 20.79%, and 84.82%, respectively. The future PGSHs of S. halepense increased continuously in the Northern Hemisphere, transferring to higher latitudes. Environmental variables were more significant than LUC in predicting the PGSHs of S. halepense. Future PGSHs of S. halepense are expected to increase, exacerbating the invasion risk through agricultural LUC. These results provide a basis for the early warning and prevention of S. halepense worldwide.
Water Deficit at Vegetative Stage Induces Tolerance to High Temperature during Anthesis in Rice
Authors: Feijó Anderson da Rosa , Viana Vívian Ebeling , Balbinot Andrisa , Fipke Marcus Vinicius , Souza Gustavo Maia , do Amarante Luciano , Avila Luis Antonio de
Published: August 2023

Background: Crop yields have been affected by many different biotic and abiotic factors. Generally, plants experience more than one stress during their life cycle, and plants can tolerate multiple stresses and develop cross-tolerance. The expected rise in atmospheric CO2 concentration ([CO2]) can contribute to cross-tolerance. Priming is a strategy to increase yield or to maintain yield under stress conditions. Thus, our objective was to evaluate if priming the rice plants with water deficit during the vegetative stage can induce tolerance to heat stress at anthesis and to evaluate the contribution of e[CO2]. Methods: The experiment was arranged in a completely randomized design in a factorial arrangement. Factor A consisted of the following treatments: water deficit at four-leaf stage (no-stress, and drought stress), heat at anthesis (normal temperature, high temperature), and priming with water deficit at four-leaf stage and heat stress at anthesis; and Factor B was two [CO2] treatments: a[CO2] = 400 ± 40 μmol mol−1 and e[CO2] = 700 ± 40 μmol mol−1. We assessed the effect of the treatments on plant growth, yield, biochemical, and transcriptome alterations. Results: Although e[CO2] affected rice growth parameters, it did not affect the priming effect. Primed plants showed an increase in yield and number of panicles per plant. Primed plants showed upregulation of OsHSP16.9A, OsHSP70.1, and OsHSP70.6. These results showed induced cross-tolerance. Conclusions: Water deficit at the rice vegetative stage reduces the effect of heat stress at the reproductive stage. Water deficit at the vegetative stage can be used, after further testing in field conditions, to reduce the effect of heat stress during flowering in rice.
Selection of Soybean and Cowpea Cultivars with Superior Performance under Drought Using Growth and Biochemical Aspects
Authors: Miranda Rafael de Souza , Fonseca Bruno Sousa Figueiredo da , Pinho Davielson Silva , Batista Jennyfer Yara Nunes , Brito Ramilos Rodrigues de , Silva Everaldo Moreira da , Ferreira Wesley Santos , Costa José Hélio , Lopes Marcos dos Santos , Sousa Renan Henrique Beserra de , Neves Larissa Fonseca , Penha José Antônio Freitas , Santos Amanda Soares , Lima Juliana Joice Pereira , Paula-Marinho Stelamaris de Oliveira , Neto Francisco de Alcântara , Aguiar Évelyn Silva de , Santos Clesivan Pereira dos , Gomes-Filho Enéas
Published: August 2023

Identifying cultivars of leguminous crops exhibiting drought resistance has become crucial in addressing water scarcity issues. This investigative study aimed to select soybean and cowpea cultivars with enhanced potential to grow under water restriction during the vegetative stage. Two parallel trials were conducted using seven soybean (AS3810IPRO, M8644IPRO, TMG1180RR, NS 8338IPRO, BMX81I81IPRO, M8808IPRO, and BÔNUS8579IPRO) and cowpea cultivars (Aracê, Novaera, Pajeú, Pitiúba, Tumucumaque, TVU, and Xique-xique) under four water levels (75, 60, 45, and 30% field capacity—FC) over 21 days. Growth, water content, membrane damage, photosynthetic pigments, organic compounds, and proline levels were analyzed. Drought stress significantly impacted the growth of both crops, particularly at 45 and 30% FC for soybean and 60 and 45% FC for cowpea plants. The BÔNUS8579IPRO and TMG1180RR soybean cultivars demonstrated the highest performance under drought, a response attributed to increased amino acids and proline contents, which likely help to mitigate membrane damage. For cowpea, the superior performance of the drought-stressed Xique-xique cultivar was associated with the maintenance of water content and elevated photosynthetic pigments, which contributed to the preservation of the photosynthetic efficiency and carbohydrate levels. Our findings clearly indicate promising leguminous cultivars that grow under water restriction, serving as viable alternatives for cultivating in water-limited environments.
Tropical Trees Will Need to Acclimate to Rising Temperatures—But Can They?
Authors: Feeley Kenneth J. , Bernal-Escobar Manuel , Fortier Riley , Kullberg Alyssa T.
Published: August 2023

For tropical forests to survive anthropogenic global warming, trees will need to avoid rising temperatures through range shifts and “species migrations” or tolerate the newly emerging conditions through adaptation and/or acclimation. In this literature review, we synthesize the available knowledge to show that although many tropical tree species are shifting their distributions to higher, cooler elevations, the rates of these migrations are too slow to offset ongoing changes in temperatures, especially in lowland tropical rainforests where thermal gradients are shallow or nonexistent. We also show that the rapidity and severity of global warming make it unlikely that tropical tree species can adapt (with some possible exceptions). We argue that the best hope for tropical tree species to avoid becoming “committed to extinction” is individual-level acclimation. Although several new methods are being used to test for acclimation, we unfortunately still do not know if tropical tree species can acclimate, how acclimation abilities vary between species, or what factors may prevent or facilitate acclimation. Until all of these questions are answered, our ability to predict the fate of tropical species and tropical forests—and the many services that they provide to humanity—remains critically impaired.
Removal of Nano-Zinc Oxide (nZnO) from Simulated Waters by C/F/S—Focusing on the Role of Synthetic Coating, Organic Ligand, and Solution Chemistry
Authors: Khan Rizwan , Inam Muhammad Ali , Yeom Ick Tae , Lee Kang Hoon , Mangi Kashif Hussain
Published: August 2023

Increased usage of nano-zinc oxide (nZnO) in different commercial fields has raised serious concerns regarding their discharge into the water streams containing natural and synthetic coating agents. Moreover, utilization of ground and surface water for drinking purposes is a common approach in many countries. Therefore, the removal of nZnO particles from water is essential to minimize the risk to the environment. The present research investigated the removal of nZnO from complex water matrices by conventional coagulation-flocculation-sedimentation (C/F/S) process using polyaluminum chloride (PACl) as coagulants. The result showed that removal of uncoated nZnO through sedimentation was efficient in waters containing divalent cations in the absence of dissolved organic matter (DOM). For the water containing higher salt concentration, PACl coagulant showed better removal performance with increasing coagulant dosage; however, synthetic organic coating agent and DOM significantly decreased the removal up to 75%. The surface potential of studied waters indicated that the addition of PACl affects the charge potential of nZnO particles resulting in charge neutralization. The result of the particle size analyzer revealed the presence of smaller particles with size of 430 nm even after C/F/S process, which may increase the possibility of particles release into aquatic environment. The results of the present study may help in understating the removal behavior of other coated nanoparticles during conventional water treatment.
Bayesian Inference for the Loss Models via Mixture Priors
Authors: Deng Min , Aminzadeh Mostafa S.
Published: August 2023

Constructing an accurate model for insurance losses is a challenging task. Researchers have developed various methods to model insurance losses, such as composite models. Composite models combine two distributions: one for part of the data with small and high frequencies and the other for large values with low frequencies. The purpose of this article is to consider a mixture of prior distributions for exponential–Pareto and inverse-gamma–Pareto composite models. The general formulas for the posterior distribution and the Bayes estimator of the support parameter θ are derived. It is shown that the posterior distribution is a mixture of individual posterior distributions. Analytic results and Bayesian inference based on the proposed mixture prior distribution approach are provided. Simulation studies reveal that the Bayes estimator with a mixture distribution outperforms the Bayes estimator without a mixture distribution and the ML estimator regarding their accuracies. Based on the proposed method, the insurance losses from natural events, such as floods from 2000 to 2019 in the USA, are considered. As a measure of goodness-of-fit, the Bayes factor is used to choose the best-fitted model.
Tree-Structured Parzan Estimator–Machine Learning–Ordinary Kriging: An Integration Method for Soil Ammonia Spatial Prediction in the Typical Cropland of Chinese Yellow River Delta with Sentinel-2 Remote Sensing Image and Air Quality Data
Authors: Song Yingqiang , Ye Mingzhu , Zheng Zhao , Zhan Dexi , Duan Wenxu , Lu Miao , Song Zhenqi , Sun Dengkuo , Yao Kaizhong , Ding Ziqi
Published: August 2023

Spatial prediction of soil ammonia (NH3) plays an important role in monitoring climate warming and soil ecological health. However, traditional machine learning (ML) models do not consider optimal parameter selection and spatial autocorrelation. Here, we present an integration method (tree-structured Parzen estimator–machine learning–ordinary kriging (TPE–ML–OK)) to predict spatial variability of soil NH3 from Sentinel-2 remote sensing image and air quality data. In TPE–ML–OK, we designed the TPE search algorithm, which encourages gradient boosting decision tree (GBDT), random forest (RF), and extreme gradient boosting (XGB) models to pay more attention to the optimal hyperparameters’ high-possibility range, and then the residual ordinary kriging model is used to further improve the prediction accuracy of soil NH3 flux. We found a weak linear correlation between soil NH3 flux and environmental variables using scatter matrix correlation analysis. The optimal hyperparameters from the TPE search algorithm existed in the densest iteration region, and the TPE–XGB–OK method exhibited the highest predicted accuracy (R2 = 85.97%) for soil NH3 flux in comparison with other models. The spatial mapping results based on TPE–ML–OK methods showed that the high fluxes of soil NH3 were concentrated in the central and northeast areas, which may be influenced by rivers or soil water. The analysis result of the SHapley additive explanation (SHAP) algorithm found that the variables with the highest contribution to soil NH3 were O3, SO2, PM10, CO, and NDWI. The above results demonstrate the powerful linear–nonlinear interpretation ability between soil NH3 and environmental variables using the integration method, which can reduce the impact on agricultural nitrogen deposition and regional air quality.
High Spatial Resolution Nighttime PM2.5 Datasets in the Beijing–Tianjin–Hebei Region from 2015 to 2021 Using VIIRS/DNB and Deep Learning Model
Authors: Ma Yu , Zhang Wenhao , Chen Xiaoyang , Zhang Lili , Liu Qiyue
Published: August 2023

The concentration of particulate matter (PM2.5) can be estimated using satellite data collected during the daytime. However, there are currently no long-term evening PM2.5 datasets, and the application of low-light satellite data to analyze nighttime PM2.5 concentrations is limited. The Visible Infrared Imaging Radiometer Suite Day/Night Band (VIIRS/DNB), meteorology, Digital Elevation Model, moon phase angle, and Normalized Digital Vegetation Index were used in this study to develop a Deep Neural Network model (DNN) for estimating the nighttime concentrations of PM2.5 in the Beijing–Tianjin–Hebei (BTH) region from 2015 to 2021. To evaluate the model’s performance from 2015 to 2021, a ten-fold cross-validation coefficient of determination was utilized (CV − R2 = 0.51 − 0.68). Using a high spatial resolution of 500 m, we successfully generated a PM2.5 concentration map for the BTH region. This finer resolution enabled a detailed representation of the PM2.5 distribution over the area. Interannual and seasonal trends in nighttime PM2.5 concentrations were analyzed. Winter had the highest seasonal spatial PM2.5, followed by spring and autumn, whereas summer had the lowest. The annual concentration of PM2.5 at night steadily decreased. Finally, the estimation of nighttime PM2.5 was applied in scenarios such as continuous day–night changes, rapid short-term changes, and single-point monitoring. A deeper understanding of PM2.5, enabled by nightly PM2.5, will serve as an invaluable resource for future research.