bluuebunny/crossref_metadata_2025 huggingface dataset:
Survival of brain tumour patients with epilepsy
Authors: Mastall Maximilian , Wolpert Fabian , Gramatzki Dorothee , Imbach Lukas , Becker Denise , Schmick Anton , Hertler Caroline , Roth Patrick , Weller Michael , Wirsching Hans-Georg
Published: May 2021

Pro-tumorigenic electrochemical synapses between neurons and brain tumour cells in preclinical studies suggest unfavourable effects of epilepsy on patient survival. We investigated associations of epilepsy and survival in three cohorts of brain tumour patients (meningioma, glioblastoma and brain metastases). Cohorts were segregated into three groups for comparative analyses: (i) no epilepsy; (ii) epilepsy without status epilepticus; and (iii) status epilepticus. Status epilepticus was considered a surrogate of extensive neuronal hyperexcitability. The main outcome was progression-free survival (meningioma) and overall survival (glioblastoma and brain metastases), adjusted for established prognostic factors and onset of epilepsy by time-dependent multivariate Cox modelling. The primary analysis population comprised 1792 patients (742 meningioma, 249 glioblastoma, 801 brain metastases). Epilepsy was associated with favourable prognostic factors. However, on multivariate analyses, status epilepticus was associated with inferior overall survival of patients with glioblastoma [status epilepticus versus no epilepsy multivariate hazard ratio (HR) 3.72, confidence interval (CI) 1.78–7.76, P < 0.001] and brain metastases (status epilepticus versus no epilepsy HR 2.30, CI 1.10–4.79, P = 0.026). Among brain metastases patients, but not among patients with meningioma or glioblastoma, epilepsy was similarly associated with inferior overall survival (epilepsy versus no epilepsy HR 2.16, CI 1.60–2.93, P < 0.001). We conclude that epilepsy may convey inferior survival of patients with malignant brain tumours.
ID4 predicts poor prognosis and promotes BDNF-mediated oncogenesis of colorectal cancer
Authors: Ha Cam-Thu , Cheng Chih-Yuan , Zheng Ming-Yi , Hsu Tang-Hui , Miao Chia-Cheng , Lee Chang-Jung , Wang Horng-Dar , Pan Shien-Tung , Chou Yu-Ting
Published: May 2021

Inhibitors of DNA binding and cell differentiation (ID) proteins regulate cellular differentiation and tumor progression. Whether ID family proteins serve as a linkage between pathological differentiation and cancer stemness in colorectal cancer is largely unknown. Here, the expression of ID4, but not other ID family proteins, was enriched in LGR5-high colon cancer stem cells. Its high expression was associated with poor pathological differentiation of colorectal tumors and shorter survival in patients. Knockdown of ID4 inhibited the growth and dissemination of colon cancer cells, while enhancing chemosensitivity. Through gene expression profiling analysis, brain-derived neurotrophic factor (BDNF) was identified as a downstream target of ID4 expression in colorectal cancer. BDNF knockdown decreased the growth and migration of colon cancer cells, and its expression enhanced dissemination, anoikis resistance and chemoresistance. ID4 silencing attenuated the epithelial-to-mesenchymal transition pattern in colon cancer cells. Gene cluster analysis revealed that ID4 and BDNF expression was clustered with mesenchymal markers and distant from epithelial genes. BDNF silencing decreased the expression of mesenchymal markers Vimentin, CDH2 and SNAI1. These findings demonstrated that ID4–BDNF signaling regulates colorectal cancer survival, with the potential to serve as a prognostic marker in colorectal cancer.
Sustainable Surveillance of Neglected Tropical Diseases for the Post-Elimination Era
Authors: Hatherell Hollie-Ann , Simpson Hope , Baggaley Rebecca F , Hollingsworth T Déirdre , Pullan Rachel L
Published: May 2021

The World Health Organization’s (WHO’s) 2030 road map for neglected tropical diseases (NTDs) emphasizes the importance of strengthened, institutionalized “post-elimination” surveillance. The required shift from disease-siloed, campaign-based programming to routine, integrated surveillance and response activities presents epidemiological, logistical, and financial challenges, yet practical guidance on implementation is lacking. Nationally representative survey programs, such as demographic and health surveys (DHS), may offer a platform for the integration of NTD surveillance within national health systems and health information systems. Here, we describe characteristics of DHS and other surveys conducted within the WHO Africa region in terms of frequency, target populations, and sample types and discuss applicability for post-validation and post-elimination surveillance. Maximizing utility depends not only on the availability of improved diagnostics but also on better understanding of the spatial and temporal dynamics of transmission at low prevalence. To this end, we outline priorities for obtaining additional data to better characterize optimal post-elimination surveillance platforms.
Risk Factors Associated With Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae Positive Cultures in a Cohort of US Veterans
Authors: Wilson Geneva M , Suda Katie J , Fitzpatrick Margaret A , Bartle Brian , Pfeiffer Christopher D , Jones Makoto , Rubin Michael A , Perencevich Eli , Evans Martin , Evans Charlesnika T ,   QUERI CARRIAGE Program
Published: May 2021

Background

Carbapenem-resistant Enterobacteriaceae (CRE) cause approximately 13 100 infections, with an 8% mortality rate in the United States annually. Carbapenemase-producing CRE (CP-CRE) a subset of CRE infections infections have much higher mortality rates (40%–50%). There has been little research on characteristics unique to CP-CRE. The goal of the current study was to assess differences between US veterans with non-CP-CRE and those with CP-CRE cultures.

Methods

A retrospective cohort of veterans with CRE cultures from 2013–2018 and their demographic, medical, and facility level covariates were collected. Clustered multiple logistic regression models were used to assess independent factors associated with CP-CRE.

Results

The study included 3096 unique patients with cultures positive for either non-CP-CRE or CP-CRE. Being African American (odds ratio, 1.44 [95% confidence interval, 1.15–1.80]), diagnosis in 2017 (3.11 [2.13–4.54]) or 2018 (3.93 [2.64–5.84]), congestive heart failure (1.35 [1.11–1.64]), and gastroesophageal reflux disease (1.39 [1.03–1.87]) were associated with CP-CRE cultures. There was no known antibiotic exposure in the previous year for 752 patients (24.3% of the included patients). Those with no known antibiotic exposure had increased frequency of prolonged proton pump inhibitor use (17.3%) compared to those with known antibiotic exposure (5.6%).

Discussion

Among a cohort of patients with CRE, African Americans, patients with congestive heart failure, and those with gastroesophageal reflux disease had greater odds of having a CP-CRE culture. Roughly 1 in 4 patients with CP-CRE had no known antibiotic exposure in the year before their positive culture.
Trends Over Time in the Risk of Adverse Outcomes Among Patients With Severe Acute Respiratory Syndrome Coronavirus 2 Infection
Authors: Ioannou George N , O’Hare Ann M , Berry Kristin , Fan Vincent S , Crothers Kristina , Eastment McKenna C , Locke Emily , Green Pamela , Shah Javeed A , Dominitz Jason A
Published: May 2021

Background

We aimed to describe trends in adverse outcomes among patients who tested positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) between February and September 2020 within a national healthcare system.

Methods

We identified enrollees in the national United States Veterans Affairs healthcare system who tested positive for SARS-CoV-2 between 28 February 2020 and 30 September 2020 (n = 55 952), with follow-up extending to 19 November 2020. We determined trends over time in incidence of the following outcomes that occurred within 30 days of testing positive: hospitalization, intensive care unit (ICU) admission, mechanical ventilation, and death.

Results

Between February and July 2020, there were marked downward trends in the 30-day incidence of hospitalization (44.2% to 15.8%), ICU admission (20.3% to 5.3%), mechanical ventilation (12.7% to 2.2%), and death (12.5% to 4.4%), which subsequently plateaued between July and September 2020. These trends persisted after adjustment for sociodemographic characteristics, comorbid conditions, documented symptoms, and laboratory tests, including among subgroups of patients hospitalized, admitted to the ICU, or treated with mechanical ventilation. From February to September, there were decreases in the use of hydroxychloroquine (56.5% to 0%), azithromycin (48.3% to 16.6%), vasopressors (20.6% to 8.7%), and dialysis (11.6% to 3.8%) and increases in the use of dexamethasone (3.4% to 53.1%), other corticosteroids (4.9% to 29.0%), and remdesivir (1.7% to 45.4%) among hospitalized patients.

Conclusions

The risk of adverse outcomes in SARS-CoV-2–positive patients decreased markedly between February and July, with subsequent stabilization from July to September. These trends were not explained by changes in measured baseline patient characteristics and may reflect changing treatment practices or viral pathogenicity.
Machine learning-based risk profile classification of patients undergoing elective heart valve surgery
Authors: Bodenhofer Ulrich , Haslinger-Eisterer Bettina , Minichmayer Alexander , Hermanutz Georg , Meier Jens
Published: May 2021

OBJECTIVES

Machine learning methods potentially provide a highly accurate and detailed assessment of expected individual patient risk before elective cardiac surgery. Correct anticipation of this risk allows for the improved counselling of patients and avoidance of possible complications. We therefore investigated the benefit of modern machine learning methods in personalized risk prediction for patients undergoing elective heart valve surgery.

METHODS

We performed a monocentric retrospective study in patients who underwent elective heart valve surgery between 1 January 2008 and 31 December 2014 at our centre. We used random forests, artificial neural networks and support vector machines to predict the 30-day mortality from a subset of 129 available demographic and preoperative parameters. Exclusion criteria were reoperation of the same patient, patients who needed anterograde cerebral perfusion due to aortic arch surgery and patients with grown-up congenital heart disease. Finally, the cohort consisted of 2229 patients with a 30-day mortality of 3.86% (86 of 2229 cases). This trial has been registered at clinicaltrials.gov (NCT03724123).

RESULTS

The final random forest model trained on the entire data set provided an out-of-bag area under the receiver operator characteristics curve (AUC) of 0.839, which significantly outperformed the European System for Cardiac Operative Risk Evaluation (EuroSCORE) (AUC = 0.704) and a model trained only on the subset of features EuroSCORE uses (AUC = 0.745).

CONCLUSIONS

Advanced machine learning methods can predict outcomes of valve surgery procedures with higher accuracy than established risk scores based on logistic regression on pre-selected parameters. This approach is generalizable to other elective high-risk interventions and allows for training models to the cohorts of specific institutions
Fluorescence microscopy datasets for training deep neural networks
Authors: Hagen Guy M , Bendesky Justin , Machado Rosa , Nguyen Tram-Anh , Kumar Tanmay , Ventura Jonathan
Published: May 2021

Background

Fluorescence microscopy is an important technique in many areas of biological research. Two factors that limit the usefulness and performance of fluorescence microscopy are photobleaching of fluorescent probes during imaging and, when imaging live cells, phototoxicity caused by light exposure. Recently developed methods in machine learning are able to greatly improve the signal-to-noise ratio of acquired images. This allows researchers to record images with much shorter exposure times, which in turn minimizes photobleaching and phototoxicity by reducing the dose of light reaching the sample.

Findings

To use deep learning methods, a large amount of data is needed to train the underlying convolutional neural network. One way to do this involves use of pairs of fluorescence microscopy images acquired with long and short exposure times. We provide high-quality datasets that can be used to train and evaluate deep learning methods under development.

Conclusion

The availability of high-quality data is vital for training convolutional neural networks that are used in current machine learning approaches.
Novel Dent disease 1 cellular models reveal biological processes underlying ClC-5 loss-of-function
Authors: Durán Mónica , Burballa Carla , Cantero-Recasens Gerard , Butnaru Cristian M , Malhotra Vivek , Ariceta Gema , Sarró Eduard , Meseguer Anna
Published: May 2021

Dent disease 1 (DD1) is a rare X-linked renal proximal tubulopathy characterized by low molecular weight proteinuria and variable degree of hypercalciuria, nephrocalcinosis and/or nephrolithiasis, progressing to chronic kidney disease. Although mutations in the electrogenic Cl−/H+ antiporter ClC-5, which impair endocytic uptake in proximal tubule cells, cause the disease, there is poor genotype–phenotype correlation and their contribution to proximal tubule dysfunction remains unclear. To further discover the mechanisms linking ClC-5 loss-of-function to proximal tubule dysfunction, we have generated novel DD1 cellular models depleted of ClC-5 and carrying ClC-5 mutants p.(Val523del), p.(Glu527Asp) and p.(Ile524Lys) using the human proximal tubule-derived RPTEC/TERT1 cell line. Our DD1 cellular models exhibit impaired albumin endocytosis, increased substrate adhesion and decreased collective migration, correlating with a less differentiated epithelial phenotype. Despite sharing functional features, these DD1 cell models exhibit different gene expression profiles, being p.(Val523del) ClC-5 the mutation showing the largest differences. Gene set enrichment analysis pointed to kidney development, anion homeostasis, organic acid transport, extracellular matrix organization and cell-migration biological processes as the most likely involved in DD1 pathophysiology. In conclusion, our results revealed the pathways linking ClC-5 mutations with tubular dysfunction and, importantly, provide new cellular models to further study DD1 pathophysiology.
Feather, But Not Plasma, Glucocorticoid Response to Artificial Light at Night Differs between Urban and Forest Blue Tit Nestlings
Authors: Dominoni Davide M , Teo Dylon , Branston Claire J , Jakhar Aryan , Albalawi Bedur Faleh A , Evans Neil P
Published: May 2021

Urbanization drives phenotypic variation in many animal species. This includes behavioral and physiological traits such as activity patterns, aggression, and hormone levels. A current challenge of urban evolutionary ecology is to understand the environmental drivers of phenotypic variation in cities. Moreover, do individuals develop tolerance to urban environmental factors, which underlie adaptative responses and contribute to the evolution of urban populations? Most available evidence comes from correlative studies and rare experiments where a single urban-related environmental factor has been manipulated in the field. Here we present the results of an experiment in which we tested for differences in the glucocorticoid (CORT) response of urban and rural blue tits nestlings (Cyanistes caeruleus) to artificial light at night (ALAN). ALAN has been suggested to alter CORT response in several animal species, but to date no study has investigated whether this effect of ALAN differs between urban and rural populations. Immediately after hatching, urban and forest broods were either exposed to 2 lux of ALAN (using an LED source mounted inside the nestbox) or received no treatment (dark control). The experiment lasted until the chicks fledged. When the chicks were 13 days old plasma samples were collected to measure baseline CORT concentrations, and feather samples to provide an integrative measure of CORT during growth. Forest birds had higher plasma CORT (pCORT) concentrations than their urban counterparts, irrespective of whether they were exposed to ALAN or not. Conversely, we found population-specific responses of feather CORT to ALAN. Specifically, urban birds that received ALAN had increased feather CORT compared with the urban dark controls, while the opposite was true for the forest birds. pCORT concentrations were negatively associated to fledging success, irrespective of population and treatment, while feather CORT was positively associated to fledging success in broods exposed to ALAN, but negatively in the dark control ones. Our results demonstrate that ALAN can play a role in determination of the glucocorticoid phenotype of wild animals, and may thus contribute to phenotypic differences between urban and rural animals.
Plasma and soft tissue pharmacokinetics of ceftolozane/tazobactam in healthy volunteers after single and multiple intravenous infusion: a microdialysis study
Authors: Al Jalali V. , Wölfl-Duchek M. , Taubert M. , Matzneller P. , Lackner E. , Dorn C. , Kratzer A. , Wulkersdorfer B. , Österreicher Z. , Zeitlinger M.
Published: May 2021

Objectives

To investigate ceftolozane/tazobactam pharmacokinetics (PK) in plasma and interstitial space fluid (ISF) of muscle and subcutaneous tissue and establish a population PK model.

Methods

Eight healthy volunteers received four IV doses of 1000/500 mg ceftolozane/tazobactam q8h in a prospective, open-labelled PK study. ISF concentration–time profiles were determined via in vivo microdialysis up to 8 h post-dose and efficacy of unbound ceftolozane and tazobactam was estimated using the time above MIC (%ƒT>MIC) and time above threshold concentration (%T>CT), respectively. A population PK model was established by merging derived plasma and soft tissue PK data.

Results

Ceftolozane reached %ƒT>MIC values of 100% in plasma, muscle and subcutaneous ISF for Enterobacteriaceae and 87%, 89% and 87%, respectively, for Pseudomonas aeruginosa. Tazobactam %T>CT was 21%, 22% and 21% in plasma, muscle and subcutaneous ISF, respectively. Plasma protein binding was 6.3% for ceftolozane and 8.0% for tazobactam. Multiple-dose ceftolozane AUC0–8 ISF/plasma ratios were 0.92 ± 0.17 in muscle and 0.88 ± 0.18 in subcutis, and tazobactam ratios were 0.89 ± 0.25 in muscle and 0.87 ± 0.21 in subcutis, suggesting substantial soft tissue penetration.

Conclusions

Tazobactam %T>CT values were distinctly below proposed target values, indicating that tazobactam might be underdosed in the investigated drug combination. However, ISF/unbound plasma ratios of ceftolozane and tazobactam support their use in soft tissue infections. A plasma and soft tissue PK model adds important information on the PK profile of ceftolozane/tazobactam. Further investigations in patients suffering from wound infections are needed to confirm these findings.
Paternal Stress and Child Outcomes in Youth with Sickle Cell Disease
Authors: Moody Kendall L
Published: May 2021

Objective

The objective of this study was to investigate the relationship between parental stress and health-related quality of life (HRQOL) among children with sickle cell disease (SCD).

Methods

A cross-sectional correlational survey research design was used for this quantitative study. One hundred-fifty patients between the ages of 8–17 years old and their caregivers were enrolled from an outpatient comprehensive sickle cell program within a hospital setting. Patients completed the Pediatric Quality of Life Scale 3.0 SCD Module, whereas parents completed the Parental Stress Scale and demographic information questionnaire. Multiple regression analysis was used to determine if parental stress scores predicted the HRQOL of children diagnosed with SCD after controlling for demographic variables.

Results

The sample included 150 patients (median age: 12 years old; female: 52%) who were diagnosed with SCD along with 150 of their caregivers. Higher levels of parental stress predicted lower HRQOL scores (p < .001).

Conclusion

As parents reported elevated levels of stress related to caring for their child with SCD, patients reported worsening HRQOL. More evidence is needed in order to determine, which constructs of parental stress had a significant impact on HRQOL. It may be helpful for healthcare workers to be aware of familial influences of reduced HRQOL among children diagnosed with SCD. Parents reporting increased stress related to raising their child with SCD may benefit from additional support and resources.
Clinical and economic impact of comprehensive medication management implementation by clinical pharmacists in an intensive care unit: a cost–benefit analysis
Authors: Rezazadeh Amir , Hajimiri Seyed Hossein , Kebriaeezadeh Abbas , Gholami Kheirollah , Hashemian Farshad , Khoshnevisan Alireza , Mohebbi Niayesh
Published: May 2021

Objectives

Critically ill patients are at risk of drug-related problems (DRPs) and healthcare-related cost. Clinical pharmacists are specifically trained in pharmacotherapy evaluation; they can identify and prevent DRPs. We aim to evaluate clinical and economic impact of clinical pharmacist by cost–benefit analysis in intensive care unit.

Methods

This was a prospective, interventional study from healthcare provider perspective which conducted over 6 months in a neurosurgery intensive care unit (ICU) of a university hospital on 162 patients. A clinical pharmacist was dedicated to implement comprehensive medication management. All pharmacotherapy problems were categorized and economic impact of clinical pharmacist’s interventions including cost–benefit ratio and net benefit in the ICU was assessed.

Key findings

A total of 1524 interventions were done. The top five pharmacotherapy-related problems were defined as, drug selection (33.3%), dose adjustment (17.32%), fluid and electrolyte management (12.99%), drug discontinuation (12.07%) and therapeutic drug monitoring (6.75%). The minimum and maximum benefit–cost ratio was 8.4:1 to 12.7:1 and net benefit was $169,205 to $266,633, respectively over the 6-month period.

Conclusions

The participation of a clinical pharmacist in a multidisciplinary ICU team by implementation of comprehensive medication management may reduce healthcare expenditures and improve drug safety.
Acute bolus obstruction following surgical treatment of paraesophageal herniation of the greater omentum
Authors: Rassam S , Steffen T , Folie P
Published: May 2021

Hiatal herniations are most commonly diagnosed during work-up for gastroesophageal reflux disease. Here, we present a patient with retrosternal pain for whom the computed tomography scan showed a lipomatous formation in the lower posterior mediastinum, and further examination indicated the origin to be paraesophageal herniation (PEH) of the greater omentum. This was confirmed by laparoscopy, the herniated part of the greater omentum was repositioned and the hiatal hernia was repaired. During recovery the patient complained of dysphagia, a common and transient postoperative occurrence, but which later proved to be a mechanical obstruction caused by a bolus. This case raises awareness of potential differential diagnoses pre- and postoperatively in conjunction with PEH, and the management of such differential diagnoses is discussed.
Multifocal neuroendocrine tumour of the small bowel presenting as an incarcerated incisional hernia: a surgical challenge in a high-risk patient
Authors: Steinkraus Kira , Andresen Julian R , Clift Ashley K , Liedke Marc O , Frilling Andrea
Published: May 2021

Neuroendocrine tumours (NET) of the small bowel present significant clinical challenges, such as their rate of metastasis at initial presentation, common multifocality and understaging even with gold standard imaging. Here, we present a case of a high-risk surgical patient with a complex medical history initially presenting as an acute abdomen due to an incarcerated incisional hernia. He was found at emergency laparotomy to have three small NET deposits in a 30-cm segment of incarcerated ileum which was resected. Postoperative morphological and functional imaging and biochemical markers were unremarkable, but due to clinical suspicion for undetected residual tumour bulk given the non-systematic palpation of the entire small bowel at initial operation, underwent re-operation where a further 70 cm of ileum was found to harbour multiple tumour deposits (n = 25) and was resected. There was no surgical morbidity and the patient remains tumour-free at 9-month follow-up.
Constraints on the sub-pc environment of the nearby Type Iax SN 2014dt from deep X-ray and radio observations
Authors: Stauffer C M , Margutti R , Linford J D , Chomiuk L , Coppejans D L , Demarchi L , Jacobson-Galán W , Bright J , Foley R J , Horesh A , Baldeschi A
Published: May 2021

We present X-ray and radio observations of what may be the closest Type Iax supernova (SN) to date, SN 2014dt (d = 12.3–19.3 Mpc), and provide tight constraints on the radio and X-ray emission. We infer a specific radio luminosity $L_R\lt (1.0\!-\!2.4)\times 10^{25}\, \rm {erg\, s^{-1}\, Hz^{-1}}$ at a frequency of 7.5 GHz and a X-ray luminosity $L_X\lt 1.4\times 10^{38}\, \rm {erg\, s^{-1}}$ (0.3–10 keV) at ∼38–48 d post-explosion. We interpret these limits in the context of Inverse Compton (IC) emission and synchrotron emission from a population of electrons accelerated at the forward shock of the explosion in a power-law distribution $N_e(\gamma _e)\propto \gamma _e^{-p}$ with p = 3. Our analysis constrains the progenitor system mass-loss rate to be $\dot{M}\lt 5.0 \times 10^{-6} \rm {M_{\odot }\, yr^{-1}}$ at distances $r\lesssim 10^{16}\, \rm {cm}$ for an assumed wind velocity $v_w=100\, \rm {km\, s^{-1}}$, and a fraction of post-shock energy into magnetic fields and relativistic electrons of ϵB = 0.01 and ϵe = 0.1, respectively. This result rules out some of the parameter space of symbiotic giant star companions, and it is consistent with the low mass-loss rates expected from He-star companions. Our calculations also show that the improved sensitivity of the next-generation Very Large Array (ngVLA) is needed to probe the very low-density media characteristic of He stars that are the leading model for binary stellar companions of white dwarfs giving origin to Type Iax SNe.
Grid of pseudo-2D chemistry models for tidally locked exoplanets – I. The role of vertical and horizontal mixing
Authors: Baeyens Robin , Decin Leen , Carone Ludmila , Venot Olivia , Agúndez Marcelino , Mollière Paul
Published: May 2021

The atmospheres of synchronously rotating exoplanets are intrinsically 3D, and fast vertical and horizontal winds are expected to mix the atmosphere, driving the chemical composition out of equilibrium. Due to the longer computation times associated with multidimensional forward models, horizontal mixing has only been investigated for a few case studies. In this paper, we aim to generalize the impact of horizontal and vertical mixing on the chemistry of exoplanet atmospheres over a large parameter space. We do this by applying a sequence of post-processed forward models for a large grid of synchronously rotating gaseous exoplanets, where we vary the effective temperature (between 400 and 2600 K), surface gravity, and rotation rate. We find that there is a dichotomy in the horizontal homogeneity of the chemical abundances. Planets with effective temperatures below 1400 K tend to have horizontally homogeneous, vertically quenched chemical compositions, while planets hotter than 1400 K exhibit large compositional day-night differences for molecules such as CH4. Furthermore, we find that the planet’s rotation rate impacts the planetary climate, and thus also the molecular abundances and transmission spectrum. By employing a hierarchical modelling approach, we assess the relative importance of disequilibrium chemistry on the exoplanet transmission spectrum, and conclude that the temperature has the most profound impact. Temperature differences are also the main cause of limb asymmetries, which we estimate could be observable with the James Webb Space Telescope. This work highlights the value of applying a consistent modelling setup to a broad parameter space in exploratory theoretical research.
Estimage: a webserver hub for the computation of methylation age
Authors: Di Lena Pietro , Sala Claudia , Nardini Christine
Published: May 2021

Methylage is an epigenetic marker of biological age that exploits the correlation between the methylation state of specific CG dinucleotides (CpGs) and chronological age (in years), gestational age (in weeks), cellular age (in cell cycles or as telomere length, in kilobases). Using DNA methylation data, methylage is measurable via the so called epigenetic clocks. Importantly, alterations of the correlation between methylage and age (age acceleration or deceleration) have been stably associated with pathological states and occur long before clinical signs of diseases become overt, making epigenetic clocks a potentially disruptive tool in preventive, diagnostic and also in forensic applications. Nevertheless, methylage dependency from CpGs selection, mathematical modelling, tissue specificity and age range, still makes the potential of this biomarker limited. In order to enhance model comparisons, interchange, availability, robustness and standardization, we organized a selected set of clocks within a hub webservice, EstimAge (Estimate of methylation Age, http://estimage.iac.rm.cnr.it), which intuitively and informatively enables quick identification, computation and comparison of available clocks, with the support of standard statistics.
New DNA-hydrolyzing DNAs isolated from an ssDNA library carrying a terminal hybridization stem
Authors: Zhang Canyu , Li Qingting , Xu Tianbin , Li Wei , He Yungang , Gu Hongzhou
Published: May 2021

DNA-hydrolyzing DNAs represent an attractive type of DNA-processing catalysts distinctive from the protein-based restriction enzymes. The innate DNA property has enabled them to readily join DNA-based manipulations to promote the development of DNA biotechnology. A major in vitro selection strategy to identify these DNA catalysts relies tightly on the isolation of linear DNAs processed from a circular single-stranded (ss) DNA sequence library by self-hydrolysis. Herein, we report that by programming a terminal hybridization stem in the library, other than the previously reported classes (I & II) of deoxyribozymes, two new classes (III & IV) were identified with the old selection strategy to site-specifically hydrolyze DNA in the presence of Zn2+. Their representatives own a catalytic core consisting of ∼20 conserved nucleotides and a half-life of ∼15 min at neutral pH. In a bimolecular construct, class III exhibits unique broad generality on the enzyme strand, which can be potentially harnessed to engineer DNA-responsive DNA hydrolyzers for detection of any target ssDNA sequence. Besides the new findings, this work should also provide an improved approach to select for DNA-hydrolyzing deoxyribozymes that use various molecules and ions as cofactors.
Experimental evidence that social information affects habitat selection in Marbled Murrelets
Authors: Valente Jonathon J , Nelson S Kim , Rivers James W , Roby Daniel D , Betts Matthew G
Published: May 2021

Habitat selection decisions can impact individual fitness and ultimately scale up to mediate population dynamics. Understanding how birds select habitat is thus critical for discerning the biological processes structuring populations and for developing conservation strategies, particularly for species in decline. Marbled Murrelet (Brachyramphus marmoratus; hereafter murrelet) populations have declined in recent decades due to loss of late-successional forest nesting habitat and changing ocean conditions that impact foraging success. Most other seabirds in the family Alcidae nest colonially and evidence suggests nesting murrelets may aggregate in stands, yet no studies have examined murrelet use of social information in nest-site selection. In 2016, we experimentally simulated the presence of murrelets at 14 randomly chosen potential breeding sites by broadcasting murrelet calls throughout the breeding period. Between broadcasting bouts, we recorded calls of wild murrelets and compared call rates with those recorded at 14 control sites (no broadcast). One year after playbacks ceased (2017) we conducted breeding season surveys to document behaviors indicative of murrelet breeding activity. Broadcasting murrelet calls in 2016 increased daily odds of wild murrelets vocalizing during the treatment period by up to 15.4× (95% CI: 2.3, 125.4) relative to control sites. During the 2017 breeding season, the odds of occupancy were 10.0× (CI: 1.2, 81.4) greater at treatment sites than control sites. These results indicate that social information influences murrelet breeding site selection because the simulated conspecific presence in potential nesting habitat appeared to attract prospectors in 2016 that continued occupying treatment sites the following year. This conspecific attraction implies murrelet nesting sites are likely to remain occupied over time and that large tracts of nesting habitat may be important for supporting murrelet populations. Murrelets may also be susceptible to information-mediated Allee effects whereby a lack of conspecific information about nesting habitat could exacerbate long-term population declines.
Ecological and evolutionary drivers of geographic variation in songs of a Neotropical suboscine bird: The Drab-breasted Bamboo Tyrant (Hemitriccus diops, Rhynchocyclidae)
Authors: Acero-Murcia Adriana Carolina , Raposo do Amaral Fábio , de Barros Fábio C , da Silva Ribeiro Tiago , Miyaki Cristina Y , Maldonado-Coelho Marcos
Published: May 2021

Understanding the evolutionary and ecological mechanisms that shape the spatial divergence of signals involved in reproductive isolation is a central goal in studies of speciation. For birds with innate songs, such as the suboscine passerine birds, the integration and comparison of both genetic and ecological factors in explaining song variation at the microevolutionary scale are rare. Here, we evaluated the evolutionary and ecological processes underlying the variation in the songs of the Atlantic Forest endemic Drab-breasted Bamboo Tyrant (Hemitriccus diops), testing the effects of both stochastic and adaptive processes, namely the stochastic and acoustic adaptation hypotheses, respectively. We combined vocal, genetic, and ecological (climate and forest cover) data across the species’ range. To this end, we analyzed 89 samples of long and short songs. We performed analyses on raw and synthetic data song variables with linear mixed models and multivariate statistics. Our results show that both song types differ in spectral features between the 2 extant phylogeographic lineages of this species, but such vocal divergence is weak and subtle in both song types. Overall, there is a positive relationship of acoustic distances with the amount of forest cover in long songs. Our results suggest that there is cryptic geographical variation in both song types and that this variation is associated with low levels of genetic divergence in both songs and with ecological factors in long songs.
From leisure to isolation: a call to explore hotel sectors’ role during COVID-19 pandemic
Authors: Aquino Jay A , Banal Jeffrey T , Pablo Felipe B , David Joy D , Sarmiento Philip Joseph D
Published: May 2021

In a recent correspondence, authors discussed the role of private companies in fulfilling their corporate social responsibility (CSR) by coming up with their own vaccination program for their employees during the COVID-19 pandemic. This paper supports the invitation for companies to act in accordance with their CSR and by emphasizing the various roles of companies just like what selected hotels do as isolation and quarantine facilities during the pandemic. However, certain considerations and issues must also be addressed by hotel sectors in accomplishing their CSR especially in time of public health crisis.
Fossil-Informed Models Reveal a Boreotropical Origin and Divergent Evolutionary Trajectories in the Walnut Family (Juglandaceae)
Authors: Zhang Qiuyue , Ree Richard H , Salamin Nicolas , Xing Yaowu , Silvestro Daniele
Published: May 2021

Temperate woody plants in the Northern Hemisphere have long been known to exhibit high species richness in East Asia and North America and significantly lower diversity in Europe, but the causes of this pattern remain debated. Here, we quantify the roles of dispersal, niche evolution, and extinction in shaping the geographic diversity of the temperate woody plant family Juglandaceae (walnuts and their relatives). Integrating evidence from molecular, morphological, fossil, and (paleo)environmental data, we find strong support for a Boreotropical origin of the family with contrasting evolutionary trajectories between the temperate subfamily Juglandoideae and the tropical subfamily Engelhardioideae. Juglandoideae rapidly evolved frost tolerance when the global climate shifted to ice-house conditions from the Oligocene, with diversification at high latitudes especially in Europe and Asia during the Miocene. Subsequent range contraction at high latitudes and high levels of extinction in Europe driven by global cooling led to the current regional disparity in species diversity. Engelhardioideae showed temperature conservatism while adapting to increased humidity, tracking tropical climates to low latitudes since the middle Eocene with comparatively little diversification, perhaps due to high competition in the tropical zone. The biogeographic history of Juglandaceae shows that the North Atlantic land bridge and Europe played more critical roles than previously thought in linking the floras of East Asia and North America, and showcases the complex interplay among climate change, niche evolution, dispersal, and extinction that shaped the modern disjunct pattern of species richness in temperate woody plants. [Boreotropical origin; climatic niche evolution; disjunct distribution; dispersal; diversity anomaly; extinction; Juglandaceae.]
Musculoskeletal Injuries in US Air Force Security Forces, January 2009 to December 2018
Authors: Sundstrom Julia N. , Webber Bryant J. , Delclos George L. , Herbold John R. , Gimeno Ruiz de Porras David
Published: May 2021

Objective:

To determine the incidence, types, and risk factors of musculoskeletal injuries in a military security forces population.

Methods:

Demographic and diagnostic data were retrieved on enlisted US Air Force security forces personnel who served on active duty between January 2009 and December 2018. Incidence rates and ratios were calculated using Poisson regression.

Results:

During 251,787 person-years of exposure, 62,489 personnel served on active duty. Of these, 40,771 (65.2%) were diagnosed with at least one musculoskeletal injury. The majority (60.1%) of the 164,078 unique musculoskeletal injuries were inflammation and pain secondary to overuse. After adjusting for other factors, women had a 31% higher injury rate than men, and those who were overweight and obese had 15% and 30% higher rates, respectively, than normal-weight peers.

Conclusions:

Modifiable and non-modifiable factors contribute to musculoskeletal injuries in the security forces career field.
Associations Between Personal Values and Work Engagement
Authors: Sato Nana , Watanabe Kazuhiro , Nishi Daisuke , Kawakami Norito
Published: May 2021

Objective:

This study aimed to investigate associations between personal values and work engagement among workers.

Methods:

This cross-sectional study used data from the Japanese Study on Stratification, Health, Income, and Neighborhood (J-SHINE) in 2010 and 2017. Personal values were measured by 11 value priorities and by commitment to these values. Multivariate linear regression analyses were conducted to determine the associations between personal values and work engagement.

Results:

A total of 1946 workers were analyzed. Four personal values (eg, “Improving society”) and commitment to values were significantly positively associated with work engagement, while “Graduating from a famous school” was significantly negatively associated.

Conclusions:

Some personal values relate to openness to change, self-transcendence, and self-enhancement, and commitment to values showed associations with work engagement. It could be beneficial to consider personal values to enhance work engagement.
Platelet count patterns and patient outcomes in sepsis at a tertiary care center
Authors: Al Saleh Khalid , AlQahtani Rakan M.
Published: May 2021

Acute physiology and chronic health evaluation II (APACHE-II) scoring system is used to classify disease severity of patients in the intensive care unit. However, several limitations render the scoring system inadequate in identifying risk factors associated with outcomes. Little is known about the association of platelet count patterns, and the timing of platelet count and other hematologic parameters in predicting mortality in patients with sepsis.

This retrospective observational study included 205 septic shock patients, with an overall mortality of 47.8%, enrolled at a tertiary care hospital in Riyadh, Kingdom of Saudi Arabia between 2018 and 2020. Bivariate and multivariate regression analyses were used to identify hematologic risk factors associated with mortality. We used the bivariate Pearson Correlation test to determine correlations between the tested variables and APACHE-II score.

Two platelet count patterns emerged: patients with a decline in platelet count after admission (group A pattern, 93.7%) and those with their lowest platelet count at admission (group B pattern, 6.3%). The lowest mean platelet count was significantly lower in nonsurvivors (105.62 ± 10.67 × 103/μL) than in survivors (185.52 ± 10.81 × 103/μL), P < .001. Bivariate Pearson correlation revealed that the lowest platelet count and platelet count decline were significantly correlated with APACHE-II score (r = −0.250, P < .01), (r = 0.326, P < .001), respectively. In multiple logistic regression analysis, the independent mortality risk factors were degree of platelet count decline in group A (odds ratio, 1.028 [95% confidence interval: 1.012–1.045], P = .001) and platelet pattern in group B (odds ratio, 6.901 [95% confidence interval: 1.446–32.932], P = .015). The patterns, values, subsets, and ratios of white blood cell count were not significantly associated with mortality.

Nadir platelet count and timing, and degree of platelet count decline are useful markers to predict mortality in early septic shock. Therefore, platelet count patterns might enhance the performance of severity scoring systems in the intensive care unit.
Ectopic tooth in maxillary sinus compressing the nasolacrimal canal
Authors: Yang Peng , Liang Hao , Zou Bo , Liu Jianlin , Yuan Daoying , Meng Zhen , Xu Kai
Published: May 2021

Rationale:

Ectopic tooth is tooth erupting out of normal anatomical position. Ectopic tooth can occur in different positions, such as maxillary sinus and nasal cavity. In this article, we present a rare case of an ectopic tooth with a dentigerous cyst in the maxillary sinus compressing the nasolacrimal canal.

Patient concerns:

An 8-year-old girl presented with a 2-month history of spontaneous lacrimation in her right eye. When she wept, more tear shed from her right eye than that from the left one. Computed tomographic (CT) imaging showed a huge low-density image containing a tooth in the maxillary sinus in her right maxilla; the right nasolacrimal canal vanished due to the compression of the ectopic tooth.

Diagnoses:

Ectopic tooth with dentigerous cyst of right maxilla, and obstruction of nasolacrimal duct

Interventions:

The patient underwent nasal endoscopic maxillary sinus cystectomy.

Outcomes:

The patient recovered well after cystectomy and has been symptom-free.

Lessons:

The unique finding is that this is the first report about ectopic tooth compressing the nasolacrimal canal and inducing spontaneous lacrimation. Treatment: aspect: surgery under endoscope is a minimally invasive approach to ectopic tooth.
Prognostic value of red blood cell distribution width in patients with acute pulmonary embolism
Authors: Liao Ye , Yang Chunsheng , Bakeer Banu
Published: May 2021

Background:

Prior reports have suggested that the red blood cell distribution width (RDW) parameter could be measured as a prognostic indicator in pulmonary embolism (PE) patients, thereby helping to guide their care. However, no systematic analyses on this topic have been completed to date, and the exact relationship between RDW and PE remains to be fully clarified. We will therefore conduct a systematic literature review with the goal of defining the correlation between RDW and mortality in acute PE cases.

Methods:

The EMBASE, Web of Knowledge, PubMed, ClinicalTrials.gov, and Cochrane Library databases will be searched for all relevant studies published from inception through March 2021 using the following search strategy: (“red blood cell distribution width”) AND (“pulmonary embolism”). Two authors will independently identify eligible studies and extract data. The Q and I
2 statistics will be used to judge heterogeneity among studies.

Results:

This study will establish the relative efficacy of RDW as a metric for predicting PE patient mortality.

Conclusions:

This study will offer a reliable, evidence-based foundation for the clinical utilization of RDW as a tool for gauging mortality risk in acute PE patients.

Ethics and dissemination:

As this is a protocol for a systematic review of previously published data, no ethical approval is required. Electronic dissemination of study results will be done through a peer-review publication or represented at a related conference.
Risk factors associated with the need for oxygen therapy in patients with COVID-19
Authors: Noh Chang Suk , Kim Won-Young , Baek Moon Seong
Published: May 2021

Respiratory failure is the major cause of death in patients with coronavirus disease (COVID-19). Data on factors affecting the need for oxygen therapy in early-stage COVID-19 are limited. This study aimed to evaluate the factors associated with the need for oxygen therapy in patients with COVID-19.

This is a retrospective study of consecutive COVID-19 patients who were hospitalized between February 27 and June 28, 2020, in South Korea. Logistic regression analyses were performed to identify the factors associated with the need for oxygen therapy.

Of the 265 patients included in the study, 26 (9.8%) received oxygen therapy, and 7 of these patients (29.2%) were transferred to a step-up facility, and 3 (11.5%) died. The median age of all patients was 46 years (IQR, 30–60 years), and the median modified early warning score at admission was 1 (IQR, 1–2). In a multivariate logistic regression analysis, being a current smoker (odds ratio [OR] 7.641, 95% confidence interval [CI] 1.686–34.630, P = .008), heart rate (OR 1.053, 95% CI 1.010–1.097, P = .014), aspartate aminotransferase values (OR 1.049, 95% CI 1.008–1.092, P = .020), blood urea nitrogen levels (OR 1.171, 95% CI 1.073–1.278, P < .001), and chest radiographic findings (OR 3.173, 95% CI 1.870–5.382, P < .001) were associated with oxygen therapy.

In patients with less severe COVID-19, the need for oxygen therapy is affected by smoking and elevated values of aspartate aminotransferase and blood urea nitrogen. Further research is warranted on the risk factors for deterioration in COVID-19 to efficiently allocate medical resources.