bluuebunny/crossref_metadata_2025 huggingface dataset:
TEHIC, un Erasmus para que la interpretación del patrimonio esté presente en la la Educación Superior Europea
Authors: Madrid Díaz María Victoria
Published: June 2024

La interpretación de patrimonio cumple más de un siglo de existencia y sigue siendo una asignatura pendiente en la educación superior en Europa. La disciplina, que actualmente cuenta con un corpus teórico robusto a nivel internacional y ha demostrado ampliamente su inmensa aportación a la conservación del patrimonio, continúa sin tener un reflejo en el ámbito de la educación formal universitaria europea, salvo honrosas excepciones.
El proyecto TEHIC se propone desarrollar un programa formativo común y estandarizado sobre interpretación de patrimonio en educación superior, que contribuya al reconocimiento de la profesión del intérprete de patrimonio, nexo clave entre la preservación del patrimonio y el público, y al mismo tiempo impulse su empleabilidad. En una segunda instancia, el proyecto revertirá en la sensibilización y concienciación de la ciudadanía europea sobre el valor de un patrimonio cultural y natural común.
Para lograrlo, TEHIC ha empezado por desarrollar un manual de buenas prácticas en interpretación de patrimonio que propone, al mismo tiempo, una metodología para la creación de actividades interpretativas de calidad y nuevas líneas de investigación en este campo.
Más que un clic: acceso directo al conocimiento desde los servicios documentales del IAPH
Authors: Loza Azuaga Luisa , Guzmán Guzmán Isabel , Sánchez Galiano Carmen , Acosta Ibáñez Pilar , Viñuales Meléndez Olga , Santos Madrid José Manuel , Engelhardt Pintiado Ruth , Márquez Carmona Lourdes
Published: June 2024

En este número de la revista especial dedicado al Instituto Andaluz de Patrimonio Histórico (IAPH), se ha considerado la oportunidad de dedicar un apartado a los servicios documentales de la institución que están integrados por tres unidades de información: archivo, biblioteca y mediateca, y dos unidades de apoyo, el tesauro y una unidad de fotografía especializada en patrimonio. Todas estas unidades de trabajo se dedican a la gestión, conservación y servicio de la documentación generada o adquirida por la institución en el desarrollo de sus funciones, teniendo como uno de sus objetivos principales poner la misma a disposición de la comunidad usuaria conformada por estudiantes, investigadores, gestores, profesores o personas interesadas por cualquier aspecto relacionado con el patrimonio. A través de un sólo clic se puede acceder a una amplia tipología de fuentes documentales, desde los documentos resultados de los proyectos hasta artículos, monografías y tesis, pasando por el gran conjunto de imágenes del patrimonio cultural de Andalucía, todo disponible en acceso abierto. Cualquier persona puede acceder a los recursos que sobre un determinado objeto o contenido tienen las tres unidades de información lo que supone un conocimiento más inmersivo e integrador. Todos estos recursos, además, podrán ser descargados para su mejor lectura, comprensión y tratamiento. Nuestro repositorio supone una ventana directa a nuestros depósitos, librerías y servidores, haciendo realidad el acceso abierto al conocimiento que generamos y tras esta ventana estamos las personas encargadas de organizar, seleccionar, describir y difundir toda esa cantidad de fuentes documentales relacionadas con el patrimonio cultural.
Programa de seguimiento y mantenimiento de bienes muebles tras la intervención del IAPH
Authors: López Rey María
Published: June 2024

La conservación del patrimonio cultural no consiste únicamente en la intervención directa de conservación y restauración, sino que es indispensable acompañar dicha intervención de un programa de conservación preventiva que elimine o minimice las causas de deterioro, además de establecer un plan de seguimiento y control que incluya tareas de mantenimiento de forma periódica.
Entendiendo que la conservación y tutela del patrimonio cultural no termina con la intervención de conservación y restauración, el IAPH en su carta de servicios incluye un servicio de seguimiento y control de bienes muebles restaurados. Un servicio que sigue un método de trabajo sistemático y ordenado, basado en el seguimiento y control de los riesgos de deterioro que afectan o puedan afectar a los bienes, y que permite la priorización de actuaciones y la toma de decisiones más adecuadas en cada momento para su conservación y mantenimiento.
El seguimiento y mantenimiento de los bienes culturales permite, además, la conservación de sus valores culturales, pues consigue alcanzar un equilibrio entre la conservación de los materiales constitutivos y uso de los bienes.
Peña Cervantes, Y. (2023) De tornos y tornillos. Tecnologías de prensado de la uva y la aceituna en el mundo romano y tardoantiguo
Authors: Trapero Fernández Pedro
Published: June 2024

Reseña del libro De tornos y tornillos. Tecnologías de prensado de la uva y la aceituna en el mundo romano y tardoantiguo, de Peña Cervantes, Y. (2023).
Núñez González, M. Vocabulario arquitectónico ilustrado la casa sevillana del siglo XVI
Authors: Engelhardt Pintiado Ruth
Published: June 2024

Resumen del libro Vocabulario arquitectónico ilustrado la casa sevillana del siglo XVI, de Núñez González, M. (2023)
PELATIHAN PENERAPAN KIMIA TERAPAN BERBASIS PEMANFAATAN SUMBER DAYA ALAM KEPADA IBU RUMAH TANGGA DI DESA PADANG NIBUNG
Authors: Nugroho Setyo Eko Nugroho
Published: June 2024

Pengabdian ini memberikan gambaran kepada masyarakat Desa Padang Nibung Khususnya ibu-ibu rumah tangga terkait penerapan teknologi kimia terapan berbasis pemanfaatan sumber daya alam yang ada di Desa Padang Nibung melalui kegiatan pelatihan pembuatan virgin coconut oil (VCO) dengan metode enzimatis dan selai dari buah-buahan lokal. Kegiatan pengabdian ini menggunakan metode ceramah, diskusi dan pelatihan langsung kepada ibu rumah tangga Desa Padang Nibung tentang pembuatan VCO dengan metode enzimatis dan selai dari buah-buahan lokal. Dari kegiatan pengabdian ini didapatkan hasil bahwasanya ibu-ibu rumah tangga Desa Padang Nibung memiliki antusias yang positif terhadap kegiatan ini dilihat dari aktifnya ruang diskusi dan pemahaman yang diperoleh dari kegiatan pengabdian. Disamping iu melalui kegiatan ini diharapkan dapat menjadi peluang usaha untuk ibu rumah tangga di Desa Padang Nibung dan meningkatkan perekonomian masyarakat desa
EDUKASI MAKANAN PENDAMPING ASI (MPASI) DI DESA PADANG NIBUNG
Authors: Amiltom Epri Wahyuni
Published: June 2024

Pemeriksaan kesehatan anak seperti posyandu di desa Padang Nibung dapat dikatakan kurang optimal, karena hanya berbatas dengan satu kali pelaksanaan dalam sebulan. Hal ini menjadi kekhawatiran kami akan kurang optimalnya penyampaian informasi tentang kesehatan pada anak, khususnya tentang bahaya stunting pada anak. Kekhawatiran kami juga semakin bertambah melihat banyaknya balita dan ibu menyusui di desa Padang Nibung. Sangat diperlukan pendampingan terhadap ibu yang masih menyusui, dikarenakan masih sering terjadinya kematian bayi yang disebabkan oleh kurangnya pemahaman ibu mengenai makanan yang cocok dan baik untuk pencernaan anak yang masih menyusui. Salah satu cara untuk meminimalisir terjadinya stunting pada anak adalah dengan mengonsumsi makanan pendamping ASI (MPASI). MPASI bertujuan untuk memenuhi kebutuhan nutrisi dari bayi berumur 6 bulan ke atas dimana pada usia tersebut ASI tidak lagi cukup untuk memenuhi kebutuhan nurisi si bayi. Selain itu MPASI juga bertujuan untuk memperkenalkan bayi dengan makanan baru selain ASI. Metode yang digunakan menggunakan metode ceramah dan diskusi. Pemateri menyampaikan materi mengenai pentingnya edukasi mengenai pentingnya makanan pendamping ASI (MPASI). Dengan dilakukannya penyuluhan masyarakat dapat mengetahui edukasi makanan pendamping asi.
Permanfaatan Teknologi Informasi dan Komunikasi (TIK) yang Maksimal Untuk Mendukung Pelaksanaan Tugas Administrasi Pemerintahan di Kelurahan Sawah Lebar Baru
Authors: Andreswari Desi , Farady Coastera Funny , Yusa Mochammad
Published: June 2024

Teknologi merupakan sebuah alat yang dipakai seseorang sebagai alat bantu untuk penyelesaian tugas maupun pekerjaan. Perkembangan teknologi semakin cepat diikuti dengan perkembangan sistem informasi berbasis teknologi. Pemanfaatan Teknologi informasi dan komunikasi (TIK) dalam sistem e-government mempunyai  tugas untuk melaksanakan pelayanan administrasi kepada masyarakat dapat lebih baik, efektivitas internal organisasi pemerintahan semakin meningkat dan akses masyarakat terhadap informasi dalam lingkungan pemerintah semakin mudah. Aparat kelurahan menduduki posisi yang sangat penting dibidang administrasi karena sebagai organ pemerintahan yang paling bawah mereka mengetahui secara pasti segala kondisi dan permasalahan yang ada di wilayahnya. Oleh karena itu oleh karena itu, pelatihan ini bertujuan meningkatkan Kapasitas Diri Aparat Kelurahan dibidang Teknologi Informasi dan Komunikasi secara optimal guna memperlancar pelaksanaan tugas pemerintahan.
Kata kunci: Teknologi Informasi dan Komunikasi, Aparat kelurahan, E-government.
PENINGKATAN MUTU SDM DESA BELITAR SEBERANG MELALUI PENERAPAN E-TOURISM MENUJU OBYEK WISATA INTERNASIONAL
Authors: Amdhi Fadlul Amdhi Yul , Mesterjon  , Jumiati Siska  , Yenni Fitria  , Tiara Lestari  , Yocia Deana Putri
Published: June 2024

To realize the development of Belitar Village towards international tourism objects, active participation of the community is needed in developing the tourism sector by utilizing technology. Smart tourism-based tourism is a new ecosystem that can support and facilitate the creation of new innovations related to the use of technology and the development of smart tourism experiences. The purpose of this community service activity is to improve community skills in promoting, managing tourism and enlarging a wider industrial scale. PKM activities are carried out by providing training to tourism managers in implementing E-Tourism. The results of PKM are known to increase skills and knowledge in using Information Technology such as the use of social media, E-Commerce to support tourism management activities.
Pembuatan Pupuk Kompos Mandiri dari Limbah Organik untuk Tanaman Pekarangan Rumah di RT. 18 Kelurahan Pematang Gubernur
Authors: Supriani Fepy , Gunawan Agustin , Afrizal Yuzuar
Published: June 2024

Sampah organik adalah sisa sayur, kulit pisang, buah yang busuk, kulit bawang yang didapat dari aktifitas rumah tangga. Sampah organik kering yang memiliki air lebih sedikit yaitu kayu, ranting pohon, kayu dan daun–daun kering. Sampah organik yang tidak diolah akan menimbulkan bau busuk dan penyakit. Permasalahan sampah limbah rumah tangga juga terjadi di Perumahan RT. 18/2 kelurahan Pematang Gubernur.  Setiap rumah  di RT. 18 masih menyisahkan tanah untuk pekarangan yang oleh masyarakat ditanami berbagai macam tanaman. Jenis tanah yang kurang baik menyebabkan tanaman tidak tumbuh subur. Pengelolahan sampah organik dapat diupayakan agar menjadi budaya di tingkat lingkungan terkecil. Sampah organik berguna untuk bahan kompos pencampur media tanam. Pembuatan sampah organik dapat dibuat secara mandiri sehingga dapat digunakan langsung untuk tanaman di pekarangan rumah, dan jika dikembangkan dapat menambah nilai ekonomis pendapatan rumah tangga. Tujuan dari kegiatan pengabdian ini adalah membangkitkan kesadaran masyarakat tentang pengolahan sampah dengan sasaran ibu rumah tangga untuk memanfaatkan sampah organik dalam pembuatan kompos mandiri yang dapat digunakan langsung sebagai media tanam dipekarangan rumah. Metode yang digunakan adalah melakukan sosialisasi dan melakukan praktik langsung pembuatan pupuk kompos dari sampah organic , tanah dan bioaktivator (cairan EM4) dalam wadah compost bag. Untuk menggerakkan ibu rumah tangga untuk langsung mempraktekkan diserahkan compost bag dan cairan EM4 sebagai sarana penunjang.Hasil dari kegiatan ini adalah meningkatnya kesadaran masyarakat terhadap kebersihan lingkungan, memberikan ketrampilan dalam pembuatan kompos yang dapat digunakan langsung untuk tanaman di pekarangan rumah dan jangka panjang dapat memberi nilai ekonomis untuk masyarakat.
Kata kunci: pengelolahan sampah, pekarangan rumah, sampah organic, kompos, cairan EM4
PEMBERDAYAAN MASYARAKAT MELALUI PILOT PROJECT TEKNOLOGI PYROLISIS PEMANFAATAN BIOCHAR PADI DI KEMUMU KABUPATEN BENGKULU UTARA
Authors: Putri Elsa Lolita , Ramadani Niska , Handayani Yanolanda Suzantry , Adiyatma Novrial
Published: June 2024

Pengabdian untuk meningkatkan produktivitas dan kesejahteraan petani di Kelurahan Kemumu Kecamatan Armaja, Kabupaten Bengkulu Utara dengan menggunakan biochar berbahan limbah sekam padi. Tujuannya untuk mengkoordinir dan mensosialisasikan petani padi sawah di Kemumu dalam peningkatan produksi dan kualitas tanah pada sawah dengan memanfaatkan limbah hasil panen yang berbahan organik. Metode yang digunakan dalam kegiatan nirlaba ini adalah Participatory Rural Appraisal (PRA) untuk berbagi informasi dalam kelompok petani. Kegiatan bakti sosial dilakukan di Kelurahan Kemumu, Kecamatan Armajaya,, Provinsi Bengkulu. Kelompok sasaran adalah Gabungan Kelompok Tani dan Bebebrapa Kelompok Tani di Kemumu. Pengabdian dilaksanakan dalam bentuk observasi dan sekolah lapangan untuk melakukan bio-talk limbah sekam padi bagi anggota kelompok tani. Capaian tujuan dari kegiatan yang dilaksanakan adalah untuk meningkatkan pemahaman kelompok tani dan gabungan kelompok tani Kelurahan Kemumu tentang pemanfaatan limbah sekam padi dan pemanfaatan biochar padi untuk meningkatkan produksi dan kesuburan tanah di Kelurahan Kemumu, Kecamatan Armajaya, Kabupaten Bengkulu Utara
SOSIALISASI PENGGUNAAN SISTEM INFORMASI GURU BIMBINGAN KONSELING(BK) BERBASIS WEBSITE
Authors: AKHMAD SAYUTI AKHMAD
Published: June 2024

Kebutuhan sistem informasi meliputi hampir semua bidang kehidupan. Informasi yang akurat, cepat, serta relevan sangat dibutuhkan dalam organisasi. Namun pada kenyataannya, karena kurang atau terbatasnya penggunaan sistem informasi terkadang tidak sinkron dengan keinginan serta harapan yang ingin diwujudkan. SMK Bina Sriwijaya merupakan sekolah yang memiliki jurusan Administrasi Perkantoran, Akuntansi Komputer, Teknik Komputer Jaringan, Akomodasi Perhotelan yang perlu mengembangkan teknologi informasi yang dapat menggunakan Website untuk komunikasi antara siswa, Guru Bimbingan Konseling dan orang tua. Sistem Pelaporan pelanggaran siswa pada sekolah saat ini masih secara manual atau offline sehingga orang tua dan guru Bimbingan Konseling masih kesulitan unutk menindaklanjutik setiap pelangganran siswa. Kegiatan pengabdian masyarakat dilakukan untuk memberikan pendampingan kepada siswa, operator website, guru Bimbingan Konseling dan perwakilan dari orang tua untuk penggunaan Sisterm Informasi Berbasis Website sehingga bisa di gunakan kapan saja dan dimana saja.
Computational Thinking in Mathematics Education : A Systematic Literature Review on its Implementation and Impact on Students' Learning
Authors: Fauzi Ahmad Lutfi , Kusumah Y S , Nurlaelah Elah , Juandi Dadang
Published: June 2024

This study aims to analyze the impact of mathematics learning using Computational Thinking (CT) integration and to enlighten educators, researchers, and policy makers on the tactics, benefits, and challenges involved in incorporating CT into mathematics education by synthesizing existing research and educational initiatives. Using the systematic literature review (SLR) method with a qualitative approach, the results of this research were in the form of descriptive analysis. The database used was Scopus to filter relevant material about CT and mathematics education. Based on the search, nine publications from 2019 – 2023 were selected systematically based on the PRISMA protocol. This study's results indicated a relationship between CT and several students' mathematical abilities, such as problem-solving and improving problem-solving skills. Not only can it improve the cognitive side of students, but CT can also improve students' affective side, such as increasing creativity, confidence, and involvement in the learning process. The impact felt is not only in the micro scope (in the classroom), but can also be macro. CT can improve the quality of higher learning, so CT research can be used to determine a country's curriculum.
The XIV. European Magnetic Sensors and Actuators Conference - Book of Abstracts
Authors:   Pavol Jozef Šafárik University in Košice, Bednarčík Jozef
Published: June 2024

The European Magnetic Sensors and Actuators Conference (EMSA) is a consolidated European forum that serves to assess the status, recent progress, and development in the field of magnetic sensor technology and magnetic actuators. It was first held in 1996 in Iasi (Romania) and since then has continued every two-three years in different European cities. The aim of the conference is to generate an overview of research in magnetic sensors and actuators, to recognize their relevance in modern industry and to identify potential future collaborations. EMSA 2024 will provide an excellent opportunity to bring together scientists and engineers from universities, research institutes and industry to present and discuss their most recent results covering both fundamental and applied aspects of magnetic sensors and actuators.
Critical thinking and artificial intelligence in tandem: A nursing perspective
Authors: Prasetyo Yunus Adhy , Dedi Blacius , Ngadiran Antonius
Published: June 2024

The human race is forced to engage in a very rapid adaptation process whenever it is confronted with technological change in any sphere of life. The unabated progress of artificial intelligence (AI) has also impacted the field of critical thinking. It is fascinating that critical thinking, an essential component of intellectual intelligence in nursing, seems to be disrupted by an artificial brain-alike machine that can automatically analyze and synthesize a series of contexts. This has been an improvisation of ideas of intellectual intelligence for a very long time. Perspectives on both sides of the coin bring up interesting questions about the role that AI will play in the future, such as whether it will disrupt the critical thinking skills of nurses or whether it may be engaged as a tool to increase the critical thinking skills capability, especially in the fields of nursing care.
‘From his Fellow-countryman’: Keats's Letters Transcribed and Annotated by Benjamin Robert Haydon
Authors: Iwamoto Hiroki
Published: June 2024

This essay examines Benjamin Robert Haydon's neglected transcripts of John Keats's letters (1845–46). Haydon copied these letters to aid the writing of Richard Monckton Milnes's first biography of the poet, Life, Letters, and Literary Remains, of John Keats (1848). For reasons unknown, however, subsequent critics and scholars have consigned the transcript copies to oblivion without making available their full text (some of which is omitted in Milnes's Life). In addition to the transcripts themselves, Haydon's annotations offer a new insight into his close relationship with Keats. The material will be of interest to many Keats scholars, especially those who wish to explore the complexities of his reception in the mid-nineteenth century and beyond, as well as the friendship between the poet and the painter.
Keats and The Stranger at Inveraray
Authors: Liu Winifred
Published: June 2024

On Friday 17 July 1818, Keats and Charles Armitage Brown arrived at Inveraray in Scotland, midway through their Scottish walking tour. Here Keats, a passionate theatre-goer, saw for the first time Augustus von Kotzebue’s play The Stranger at a makeshift playhouse inside a barn. Although Keats dedicated a long letter and a poem to this experience the precise location of the barn has never been located, nor has there been discussion of how this performance of The Stranger contributed to Keats’s theatrical experience. In this article I show that the barn-theatre at Inveraray still survives, and suggest how the Stranger performance at Inveraray, heavily ridden with Scottish inflections not present in the playscript, left an impression on Keats. I also point to traces of The Stranger found in later poems such as ‘La Belle Dame sans Merci’.
A Review Study on the Sources of Biosynthesis of Nanoparticles from Different Organisms and Their Applications in Nanomedicine
Authors: Farhat Hafiza , Iqra Kainat , Ullah Shahid
Published: June 2024

Nowadays nanoparticles is gaining much attention across the world among the researchers. From last few decades, green nanotechnology also emerging as a significant approach in order to synthesize and fabricate the nanoparticles from different organisms. This green route stabilizing the agents from biological agent for the formation of nanoparticles. The present review article aims is to elaborate the ongoing emergence of nanoparticle formulation from different microbiota. The microbe based research often used for the generation of nanoparticles which include algae, yeast, fungi, bacteria, and plants. This study theoretically based on the different mechanisms involved in the formation of most commonly studied nanoparticles. The synthesized nanoparticles from different microbes widely used in pharmaceutical products as anti-biofilm and antimicrobial agents, targeted the delivery of anticancer drugs, biosensors, water electrolytes, treatment of wastewater, degradation of dyes, biocatalysis and protection of crops against various phytopathogens. This review will also discuss in detail study about different aspects of microbial modes of nanoparticle synthesis and the mechanism behind their synthesis by utilizing of various bioreducing agents. For a large scale production, thorough study of the molecular mechanism is the need of the hour in order to develop a technology. This study’s aim is to discusses the advantages of various approaches in nanoparticle synthesis and to highlights the recent milestones achieved with future perspectives of nanoparticles. A detail study is also required in order to acknowledge the researcher that constantly focus on this green revolution.
Human Biting Plant Bug Campyloneura virgula (Hemiptera: Miridae): First Case Report in Türkiye
Authors: Zarrabi Ahrabi Salar , Şakacı Zafer , Akyıldız Gürkan , Gargılı Ayşen , Talay Şengül , Kar Sırrı
Published: June 2024

Campyloneura virgula is a plant bug found in many parts of the world. While information about the human biting of this insect is available in the literature, this is the first case report of such an incident in Türkiye. The patient was a 44-year-old man who was bitten by Campyloneura virgula in June of 2021. Clinical signs were recorded, and the bite's effects were reduced within a few days. Possible reasons and outcomes of the incident were discussed.
Extremely high spatiotemporal resolution microscopy for live cell imaging by single photon counting, noise elimination, and a novel restoration algorithm based on probability calculation
Authors: Miyashiro Daisuke , Tojima Takuro , Nakano Akihiko
Published: June 2024

Optical microscopy is essential for direct observation of dynamic phenomena in living cells. According to the classic optical theories, the images obtained through light microscopes are blurred for about half the wavelength of light, and therefore small structures below this “diffraction limit” were thought unresolvable by conventional optical microscopy. In reality, accurately obtained optical images contain complete information about the observed objects. Temporal resolution is also important for the observation of dynamic phenomena. A challenge exists here to overcome the trade-off between the time required for measurement and the accuracy of the measurement. The present paper describes a concrete methodology for reconstructing the structure of an observed object, based on the information contained in the image obtained by optical microscopy. It is realized by accurate single photon counting, complete noise elimination, and a novel restoration algorithm based on probability calculation. This method has been implemented in the Super-resolution Confocal Live Imaging Microscopy (SCLIM) we developed. The new system named SCLIM2M achieves unprecedented high spatiotemporal resolution. We have succeeded in capturing sub-diffraction-limit structures with millisecond-level dynamics of organelles and vesicles in living cells, which were never observed by conventional optical microscopy. Actual examples of the high-speed and high-resolution 4D observation of living cells are presented.
Possible involvement of sialidase and sialyltransferase activities in a stage-dependent recycling of sialic acid in some organs of type 1 and type 2 diabetic rats
Authors: Erhabor Osas Graham , Obochi Peter , Isah Murtala Bindawa , Usman Mohammed Aliyu , Umar Ismaila Alhaji , Simelane Mthokozisi B. C. , Shuaibu Mohammed Nasir , Islam Md. Shahidul , Ibrahim Mohammed Auwal
Published: June 2024

Background

Type 1 (T1D) and type 2 (T2D) diabetes lead to an aberrant metabolism of sialoglycoconjugates and elevated free serum sialic acid (FSSA) level. The present study evaluated sialidase and sialyltranferase activities in serum and some organs relevant to diabetes at early and late stages of T1D and T2D.

Methods

Sialic acid level with sialidase and sialyltransferase activities were monitored in the serum, liver, pancreas, skeletal muscle and kidney of diabetic animals at early and late stages of the diseases.

Results

The FSSA and activity of sialidase in the serum were significantly increased at late stage of both T1D and T2D while sialic acid level in the liver was significantly decreased in the early and late stages of T1D and T2D, respectively. Furthermore, the activity of sialidase was significantly elevated in most of the diabetes-relevant organs while the activity of sialyltransferase remained largely unchanged. A multiple regression analysis revealed the contribution of the liver to the FSSA while pancreas and kidney contributed to the activity of sialidase in the serum.

Conclusions

We concluded that the release of hepatic sialic acid in addition to pancreatic and renal sialidase might (in)directly contribute to the increased FSSA during both types of diabetes mellitus.
Prevalence and risk factors predisposing low bone mineral density in patients with thalassemia
Authors: Ananvutisombat Natnicha , Tantiworawit Adisak , Punnachet Teerachat , Hantrakun Nonthakorn , Piriyakhuntorn Pokpong , Rattanathammethee Thanawat , Hantrakool Sasinee , Chai-Adisaksopha Chatree , Rattarittamrong Ekarat , Norasetthada Lalita , Fanhchaksai Kanda , Charoenkwan Pimlak
Published: June 2024

Background

A common complication of thalassemia is secondary osteoporosis. This study aimed to assess the prevalence and factors associated with low BMD in thalassemic patients.

Method

This is a cross-sectional study. Eligible patients were males aged within 18–49 years or premenopausal women diagnosed with thalassemia in Chiang Mai University Hospital between July 2021 and July 2022. The diagnosis of low BMD by dual-energy x-ray absorptiometry (DXA) was defined as a Z-score of -2.0 SD or lower in either the lumbar spine or femoral neck. Clinical factors associated with low BMD were analyzed using a logistic regression model.

Results

Prevalence of low BMD was 62.4% from 210 patients with a mean age of 29.7 ± 7.6 years. The predominant clinical characteristics of low BMD thalassemia patients were being female, transfusion-dependent (TDT) and a history of splenectomy. From multivariable analysis, the independent variables associated with low BMD were transfusion dependency (odds ratio, OR 2.36; 95%CI 1.28 to 4.38; p=0.006) and body mass index (BMI) (OR 0.71; 95%CI 0.61 to 0.82; p<0.001). Among patients with low BMD, we observed a correlation between a Z-score with low IGF-1 levels (β=-0.42; 95% CI -0.83 to -0.01; p=0.040), serum phosphate levels (β=0.40; 95% CI 0.07 to 0.73; p=0.016) and hypogonadism (β=-0.48, 95% CI -0.91 to -0.04, p=0.031).

Conclusion

This study found a prevalence of low BMD in 62.4% of subjects. Factors associated with low BMD were TDT and BMI. Within the low BMD subgroup, hypogonadism, serum phosphate and low serum IGF-1 levels were associated with a lower Z-score.
A rare missense p.C125Y mutation in the TNFRSF1A gene identified in a Chinese family with tumor necrosis factor receptor-associated periodic fever syndrome
Authors: Qian Mengqing , Zhou Jingyu , Wu Jing , Zhang Haocheng , Yu Shenglei , Xu Haoxin , Yang Yixuan , Zhou Feiran , Yang Qingluan , Shao Lingyun , Zhang Wenhong , Jiang Ning , Ruan Qiaoling
Published: June 2024

Background

Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) is a rare autosomal dominant disorder with a low incidence in Asia. The most frequent clinical manifestations include fever, rash, myalgia, joint pain and abdominal pain. Misdiagnosis rates are high because of the clinical and genetic variability of the disease. The pathogenesis of TRAPS is complex and yet to be fully defined. Early genetic diagnosis is the key to precise treatment.

Methods

In this study, a Chinese family with suspected TRAPS were analyzed by genome-wide SNP genotyping, linkage analysis and targeted sequencing for identification of mutations in causative genes. To study the pathogenicity of the identified gene mutation, we performed a conservation analysis of the mutation site and protein structure analysis. Flow cytometry was used to detect TNFRSF1A shedding and quantitative real-time PCR were used to assess the activation of unfolded protein response (UPR) in the mutation carriers and healthy individuals.

Results

A typical TRAPS family history, with a pattern of autosomal dominant inheritance, led to the identification of a rare mutation in the TNFRSF1A gene (c.G374A [p.Cys125Tyr]) with unknown significance. The patient responded well to corticosteroids, and long-term therapy with colchicine effectively reduced the inflammatory attacks. No amyloid complications occurred during the 6-year follow-up. In silico protein analysis showed that the mutation site is highly conversed and the mutation prevents the formation of intrachain disulfide bonds in the protein. Despite a normal shedding of the TNFRSF1A protein from stimulated monocytes in the TRAPS patients with p.C125Y mutation, the expression of CHOP and the splicing of XBP1 was significantly higher than healthy controls, suggesting the presence of an activation UPR.

Conclusion

This is the first report of a Chinese family with the rare p.C125Y mutation in TNFRSF1A. The p.C125Y mutation does not result in aberrant receptor shedding, but instead is associated with an activated UPR in these TRAPS patients, which may provide new insights into the pathogenesis of this rare mutation in TRAPS.
Application of whole exome sequencing in carrier screening for high-risk families without probands
Authors: Huang Qinlin , Wang Zhongjie , Teng Yanling , Zhang Wen , Wen Juan , Zhu Huimin , Liang Desheng , Wu Lingqian , Li Zhuo
Published: June 2024

Purpose

This study aimed to screen the genetic etiology for the high-risk families including those with an adverse pregnancy history, a history of consanguineous marriages, or a history of genetic diseases, but lack of proband via whole exome sequencing (WES).

Methods

128 individuals from high-risk family were tested by WES. The candidate variants were analyzed according to the ACMG criteria to screen the potential carriers. At-risk couples (ARCs) who harbored the same causative gene were provided with precise fertility guidance to avoid the birth of children with birth defects.

Results

The total detection rate was 36.72%, with pathogenic/likely pathogenic (P/LP) variants found in 47 individuals, and variants of uncertain significance (VUS) were found in 34. Among couples with adverse pregnancy history: P/LP variants were found in 38 individuals, and VUS were found in 26, for a detection rate of 34.55%; among members of family history of genetic disease or consanguineous marriages: P/LP variants were found in nine individuals, and VUS were found in 8, for a detection rate of 50.00%. Otherwise, we detected 19 ARCs who both carried P/LP variants in the same gene, with a theoretical offspring prevalence of up to 7.42%.

Conclusion

In the absence of probands, carrier screening using WES can provide an efficient tool for screening the molecular etiology of high-risk families.
The impact of interleukin-6 (IL-6) and mesenchymal stem cell-derived IL-6 on neurological conditions
Authors: Kerkis Irina , Silva Álvaro Prieto da , Araldi Rodrigo Pinheiro
Published: June 2024

Interleukin-6 (IL-6) is a versatile cytokine crucial for immune response modulation, inflammation regulation, and various physiological processes in the body. Its wide-ranging functions underscore its importance in maintaining health. Dysregulated IL-6 is closely associated with many diseases, making it a key research and therapeutic target. Elevated IL-6 levels in the central nervous system worsen neuroinflammation in neurodegenerative diseases by activating microglia and astrocytes and releasing pro-inflammatory cytokines and neurotoxic molecules. Moreover, dysregulated IL-6 weakens the blood-brain barrier, exacerbating neuroinflammation and neuronal damage by allowing peripheral immune cells and inflammatory mediators to enter the brain. Mesenchymal stem cells (MSCs) show promise in modulating neuroinflammation by regulating IL-6 levels. They effectively suppress pro-inflammatory cytokines, including IL-6, while promoting anti-inflammatory factors. This therapeutic approach highlights the importance of targeting IL-6 and other inflammatory mediators to alleviate neuroinflammation and its adverse effects on neurological disorders. This review provides a comprehensive overview of IL-6’s involvement in neurological disorders, examining endogenous IL-6 and IL-6 derived from MSCs. We explore IL-6’s mechanisms affecting neuronal function, survival, and immune modulation in the central nervous system. Additionally, we discuss the potential of MSC-derived IL-6 in neuroregeneration and neuroprotection. By elucidating IL-6’s interplay with neurological pathologies, this review offers insights into novel therapeutic strategies targeting IL-6 signaling pathways for neurological disorders.
Visualization techniques of grease fluidity
Authors: Sakai Kazumi
Published: June 2024

Energy-saving technology has become increasingly significant as one of the carbon-neutral options for suppressing recent global warming. Grease-lubricated bearings have been used in various automotive and industrial machinery, requiring low torque and long service life for energy-saving performance, which is greatly influenced by grease fluidity. A numerical approach for understanding grease fluidity is very complex since grease is a non-Newtonian fluid with thixotropic properties. Visualization technique is one of the helpful methods to understand the complex grease fluidity and apply it to practical use. This paper describes state-of-the-art visualization techniques, such as fluorescence method, particle imaging velocimetry, infrared spectroscopy, X-rays, and neutron beams.
Brain organoid as a model to study the role of mitochondria in neurodevelopmental disorders: achievements and weaknesses
Authors: Coronel Raquel , García-Moreno Enrique , Siendones Emilio , Barrero Maria J. , Martínez-Delgado Beatriz , Santos-Ocaña Carlos , Liste Isabel , Cascajo-Almenara M. V.
Published: June 2024

Mitochondrial diseases are a group of severe pathologies that cause complex neurodegenerative disorders for which, in most cases, no therapy or treatment is available. These organelles are critical regulators of both neurogenesis and homeostasis of the neurological system. Consequently, mitochondrial damage or dysfunction can occur as a cause or consequence of neurodevelopmental or neurodegenerative diseases. As genetic knowledge of neurodevelopmental disorders advances, associations have been identified between genes that encode mitochondrial proteins and neurological symptoms, such as neuropathy, encephalomyopathy, ataxia, seizures, and developmental delays, among others. Understanding how mitochondrial dysfunction can alter these processes is essential in researching rare diseases. Three-dimensional (3D) cell cultures, which self-assemble to form specialized structures composed of different cell types, represent an accessible manner to model organogenesis and neurodevelopmental disorders. In particular, brain organoids are revolutionizing the study of mitochondrial-based neurological diseases since they are organ-specific and model-generated from a patient’s cell, thereby overcoming some of the limitations of traditional animal and cell models. In this review, we have collected which neurological structures and functions recapitulate in the different types of reported brain organoids, focusing on those generated as models of mitochondrial diseases. In addition to advancements in the generation of brain organoids, techniques, and approaches for studying neuronal structures and physiology, drug screening and drug repositioning studies performed in brain organoids with mitochondrial damage and neurodevelopmental disorders have also been reviewed. This scope review will summarize the evidence on limitations in studying the function and dynamics of mitochondria in brain organoids.
Early versus delayed enteral nutrition in ICU patients with sepsis: a propensity score-matched analysis based on the MIMIC-IV database
Authors: Xu Fuchao , Xu Jianxin , Ma Jinjin , Xu Wenbo , Gu Shuangshuang , Lu Geng , Wang Jun
Published: June 2024

Background

Early enteral nutrition (EN) is recommended for sepsis management, but its optimal timing and clinical benefits remain uncertain. This study evaluates whether early EN improves outcomes compared to delayed EN in patients with sepsis.

Methods

We analyzed data of septic patients from the MIMIC-IV 2.2 database, focusing on those in the Medical Intensive Care Unit (MICU) and Surgical Intensive Care Unit (SICU). Patients who initiated EN within 3 days were classified into the early EN group, while those who started EN between 3 and 7 days were classified into the delayed EN group. Propensity score matching was used to compare outcomes between the groups.

Results

Among 1,111 patients, 786 (70.7%) were in the early EN group and 325 (29.3%) were in the delayed EN group. Before propensity score matching, the early EN group demonstrated lower mortality (crude OR = 0.694; 95% CI: 0.514–0.936; p = 0.018) and shorter ICU stays (8.3 [5.2, 12.3] vs. 10.0 [7.5, 14.2] days; p < 0.001). After matching, no significant difference in mortality was observed. However, the early EN group had shorter ICU stays (8.3 [5.2, 12.4] vs. 10.1 [7.5, 14.2] days; p < 0.001) and a lower incidence of AKI stage 3 (49.3% vs. 55.5%; p = 0.030). Subgroup analysis revealed that early EN significantly reduced the 28-day mortality rate in sepsis patients with lactate levels ≤4 mmol/L, with an adjusted odds ratio (aOR) of 0.579 (95% CI: 0.361, 0.930; p = 0.024).

Conclusion

Early enteral nutrition may not significantly reduce overall mortality in sepsis patients but may shorten ICU stays and decrease the incidence of AKI stage 3. Further research is needed to identify specific patient characteristics that benefit most from early EN.
Sodium and potassium excretion and its association with cardiovascular disorders in Mexican adults
Authors: Campos Nonato Ismael , Mendoza Kenny , Vargas Meza Jorge , Flores Aldana Mario , Barquera Simón
Published: June 2024

Worldwide, as well as in Mexico, the leading cause of death is cardiovascular disease (CVD). Hypertension is the main risk factor for CVD; about 50% of the adult population suffers from this condition. High sodium (Na) intake combined with low potassium (K) intake can trigger cardiovascular disorders such as high blood pressure (BP). The aim of this study was to estimate the mean excretion of Na and K in Mexican adults using a spot urine sample, and its association with cardiovascular disorders. Information on 2,778 adults, 20–59 years of age, who participated in ENSANUT-2016 was analyzed. Na and K were estimated using Tanaka formulae. Biomarkers such as glucose, total cholesterol, triglycerides, HDL cholesterol and LDL cholesterol, and anthropometry were measured. Mean Na was 3,354 mg/day (95%CI: 3,278, 3,429), 1,440 mg/day of K (95%CI: 1,412, 1,469), and the Na-K ratio was 2.4. The excretion of Na was greater in adults with high BP (3,542 mg/day) compared to those with normal BP (3,296 mg/day). In adults with hypertension, excretion of K was 10% greater (1,534 mg/day) than in adults with normal BP (1,357 mg/day). In adults with moderate reduction of renal function, Na excretion was 22% less (2,772 mg/day) than in adults with normal kidney function (3,382 mg/day). The results of this study show that the cardiovascular health of Mexican adults is at risk, as they showed high Na excretion and low K excretion.
Development and validation of a nomogram to predict poor efficacy of imatinib in the treatment of newly diagnosed chronic phase chronic myeloid leukemia patients
Authors: Li Yuxin , Zhang Yilin , Wang Jin , He Aili , Zhang Wanggang , Cao Xingmei , Chen Yinxia , Liu Jie , Zhang Pengyu , Wang Jianli , Zhao Wanhong , Yang Yun , Meng Xin , Chen Sheping , Zhang Longjin , Wang Ting , Wang Xugeng , Ma Xiaorong
Published: June 2024

Background

Imatinib is the most widely used tyrosine kinase inhibitor (TKI) in patients with newly diagnosed chronic-phase chronic myeloid leukemia(CML-CP). However, failure to achieve optimal response after imatinib administration, and subsequent switch to second-generation TKI therapy results in poor efficacy and induces drug resistance. In the present study, we developed and validated a nomogram to predict the efficacy of imatinib in the treatment of patients newly diagnosed with CML-CP in order to help clinicians truly select patients who need 2nd generation TKI during initial therapy and to supplement the risk score system.

Methods

We retrospectively analyzed 156 patients newly diagnosed with CML-CP who met the inclusion criteria and were treated with imatinib at the Second Affiliated Hospital of Xi’an Jiao Tong University from January 2012 to June 2022. The patients were divided into a poor-response cohort (N = 60)and an optimal-response cohort (N = 43) based on whether they achieved major molecular remission (MMR) after 12 months of imatinib treatment. Using univariate and multivariate logistic regression analyses, we developed a chronic myeloid leukemia imatinib-poor treatment (CML-IMP) prognostic model using a nomogram considering characteristics like age, sex, HBG, splenic size, and ALP. The CML-IMP model was internally validated and compared with Sokal, Euro, EUTOS, and ELTS scores.

Results

The area under the curve of the receiver operator characteristic curve (AUC)of 0.851 (95% CI 0.778–0.925) indicated satisfactory discriminatory ability of the nomogram. The calibration plot shows good consistency between the predicted and actual observations. The net reclassification index (NRI), continuous NRI value, and the integrated discrimination improvement (IDI) showed that the nomogram exhibited superior predictive performance compared to the Sokal, EUTOS, Euro, and ELTS scores (P < 0.05). In addition, the clinical decision curve analysis (DCA) showed that the nomogram was useful for clinical decision-making. In predicting treatment response, only Sokal and CML-IMP risk stratification can effectively predict the cumulative acquisition rates of CCyR, MMR, and DMR (P<0.05).

Conclusion

We constructed a nomogram that can be effectively used to predict the efficacy of imatinib in patients with newly diagnosed CML-CP based on a single center, 10-year retrospective cohort study.
Case report: Precise NGS and combined bevacizumab promote durable response in ALK-positive lung adenocarcinoma with multiple-line ALK-TKI resistance
Authors: Xiong Jin , Xia Lei
Published: June 2024

Liquid biopsies including pleural fluid or plasma are commonly applied for patients with advanced non-small cell lung cancer (NSCLC) and pleural effusion (PE) to guide the treatment. ALK-TKIs are the first options for patients with ALK-positive mutations and combining ALK-TKIs with angiogenic agents may improve survival. We report here one case with ALK-positive lung adenocarcinoma in which the patient achieved a prolonged progression-free survival (PFS) of 97 months after undergoing precise pleural effusion NGS and receiving combined bevacizumab treatment following multiple-line ALK-TKI resistance.
Tropine exacerbates the ventilatory depressant actions of fentanyl in freely-moving rats
Authors: Getsy Paulina M. , May Walter J. , Young Alex P. , Baby Santhosh M. , Coffee Gregory A. , Bates James N. , Hsieh Yee-Hsee , Lewis Stephen J.
Published: June 2024

Our lab is investigating the efficacy profiles of tropine analogs against opioid-induced respiratory depression. The companion manuscript reports that the cell-permeant tropeine, tropine ester (Ibutropin), produces a rapid and sustained reversal of the deleterious actions of fentanyl on breathing, alveolar-arterial (A-a) gradient (i.e., index of alveolar gas exchange), and arterial blood-gas (ABG) chemistry in freely-moving male Sprague Dawley rats, while not compromising fentanyl analgesia. We report here that in contrast to Ibutropin, the injection of the parent molecule, tropine (200 μmol/kg, IV), worsens the adverse actions of fentanyl (75 μg/kg, IV) on ventilatory parameters (e.g., frequency of breathing, tidal volume, minute ventilation, peak inspiratory and expiratory flows, and inspiratory and expiratory drives), A-a gradient, ABG chemistry (e.g., pH, pCO2, pO2, and sO2), and sedation (i.e., the righting reflex), while not affecting fentanyl antinociception (i.e., the tail-flick latency) in freely-moving male Sprague Dawley rats. These data suggest that tropine augments opioid receptor-induced signaling events that mediate the actions of fentanyl on breathing and alveolar gas exchange. The opposite effects of Ibutropin and tropine may result from the ability of Ibutropin to readily enter peripheral and central cells. Of direct relevance is that tropine, resulting from the hydrolysis of Ibutropin, would combat the Ibutropin-induced reversal of the adverse effects of fentanyl. Because numerous drug classes, such as cocaine, atropine, and neuromuscular blocking drugs contain a tropine moiety, it is possible that their hydrolysis to tropine has unexpected/unintended consequences. Indeed, others have found that tropine exerts the same behavioral profile as cocaine upon central administration. Together, these data add valuable information about the pharmacological properties of tropine.