bluuebunny/crossref_metadata_2025 huggingface dataset:
Light use in the plant factory: changes in chlorphyll fluorescence and gas exchange parameters of two lettuce cultivars at LED spectra in floating hydroponics
Authors: Roosta Hamid Reza , Soufi Hamid Reza , Kalaji Hazem M. , Malekzadeh Khalil , Hamidpour Mohsen
Published: November 2022

Artificial light source is one of the most important factors for high quality and quantity vegetable production in plant factory. With the aim of investigating the role of light spectra on chlorophyll fluorescence, photosynthesis, and stomata parameters in lettuce plants grown in plant factory, a factorial experiment was conducted based on a completely randomized design with two lettuce cultivars (Rossa and Bionda) and four spectra LED illumination red (656 nm), red/blue (3:1) (656 nm), blue (450 nm), and white (449 nm). The results showed that blue and white LED light had the most significant effect on chlorophyll fluorescence parameters Fo, Fm, Fv, Area and PIabs in both lettuce cultivars compared to the other light treatments. The results also showed that the combination of red and blue LED light had the highest effect on stomatal conductance (gs), number of stomata, length and width of stomata in both lettuce cultivars. On the other hand, the maximum substomatal CO2 concentration (Ci) was observed in both lettuce cultivars when treated with red LED light. Also, transpiration rate (E) increased with the combination of red and blue LED in Rossa cultivar and red LED in Bionda cultivar. The results also showed that the maximum CO2 assimilation rate (A) was observed in Rossa cultivar under white LED and in Bionda cultivar under blue LED light. It is concluded that chlorophyll fluorescence characteristics and photosynthetic and stomatal properties can be affected by different spectra and cultivars of lettuce.
Intraoral Occlusal Adjustment Time and Volume Required for CAD/CAM Crowns Fabricated Using Different Virtual Mounting Methods (A Randomized Clinical Trial)
Authors: Elkady Aly , Ameen Shereen , Sami Rasha
Published: November 2022

OBJECTIVE: To measure the required clinical time and volume of occlusal adjustment when the maxillary cast in a virtual articulator is set using one of three methods: a face scan, average value alignment and digitization of a conventionally mounted articulator.
MATERIALS AND METHODS: In this randomised, triple blind, crossover trial; 11 molars requiring a single crown restoration were enrolled. Three crowns were designed and milled for every participant molar. Each of the three crowns was fabricated with the participant’s casts virtually mounted utilizing a different virtual mounting method. An impression was taken of the crown in place prior to occlusal adjustment. Occlusal adjustment was then performed and timed. After the occlusal adjustment, an impression of the adjusted crown was taken. The pre-adjustment and post-adjustment impression were superimposed and volumetrically analyzed to determine the volume of occlusal adjustment performed. Kruskal-Wallis test was used to compare between the groups.
RESULTS: No statistically significant difference in occlusal adjustment time or volume was found between crowns fabricated with different virtual mounting methods.
CONCLUSIONS: A simplified approach in virtual articulator mounting appears to be justified in construction of single full coverage prosthesis. Added labor, time and cost of more elaborate virtual mounting methods seem to be counterproductive.
Targeting epigenetic features in clear cell sarcomas based on patient-derived cell lines
Authors: Karner Christina , Anders Ines , Vejzovic Djenana , Szkandera Joanna , Scheipl Susanne , Deutsch Alexander J A , Weiss Larissa , Vierlinger Klemens , Kolb Dagmar , Kühberger Stefan , Heitzer Ellen , Habisch Hansjörg , Zhang Fangrong , Madl Tobias , Reininger-Gutmann Birgit , Liegl-Atzwanger Bernadette , Rinner Beate
Published: November 2022

Background: Clear cell sarcomas (CCSs) are translocated aggressive malignancies, most commonly affecting young adults with a high incidence of metastasis and poor prognosis. Research into the disease is more feasible when adequate models are available. By establishing CCS cell lines from a primary and metastatic lesion and isolating healthy fibroblasts from the same patient, the in vivo process is accurately reflected and aspects of clinical multistep carcinogenesis recapitulated.
Methods: Isolated tumor cells and normal healthy skin fibroblasts from the same patient were compared in terms of growth behavior and morphological characteristics using light and electron microscopy. Tumorigenicity potential was determined by soft agar colony formation assay and in vivo xenograft applications. While genetic differences between the two lineages were examined by copy number variation profiles, nuclear magnetic resonance spectroscopy determined arginine methylation as epigenetic features. Potential anti-tumor effects of a protein arginine N-methyltransferase type I (PRMT1) inhibitor were elicited in 2D and 3D cell culture experiments using cell viability and apoptosis assays. Statistical significance was calculated by one-way ANOVA and unpaired t-test.
Results: The two established CCS cell lines named MUG Lucifer prim and MUG Lucifer met showed differences in morphology, genetic and epigenetic data, reflecting the respective original tissue. The detailed cell line characterization especially in regards to the epigenetic domain allows investigation of new innovative therapies. Based on the epigenetic data, a PRMT1 inhibitor was used to demonstrate the targeted antitumor effect; normal tissue cells isolated and immortalized from the same patient were not affected with the IC50 used.
Conclusions: MUG Lucifer prim, MUG Lucifer met and isolated and immortalized fibroblasts from the same patient represent an ideal in vitro model to explore the biology of CCS. Based on this cell culture model, novel therapies could be tested in the form of PRMT1 inhibitors, which drive tumor cells into apoptosis, but show no effect on fibroblasts, further supporting their potential as promising treatment options in the combat against CCS. The data substantiate the importance of tailored therapies in advanced metastatic stage of CCS.
Longitudinal surveillance of serum titanium ion levels in patients with indigenous 3D printed total temporomandibular joint replacement
Authors: Khandelwal Garima , Roychoudhury Ajoy , Bhutia Ongkila , Shariff A.
Published: November 2022

Objective: The purpose of this longitudinal study was to surveil the serum titanium ion levels at various time intervals in patients with indigenous 3D-printed total temporomandibular joint replacement (TMJ TJR).
Study design: The study was conducted on 11 patients (male: 8; female: 3) who had undergone unilateral or bilateral TMJ TJR. Blood samples were drawn preoperatively (T0), 3 months (T1), 6 months (T2), and 1 year (T3) postoperatively. Data were analyzed and a p value of <0.05 was considered statistically significant.
Results: The mean serum titanium ion levels at T0, T1, T2, and T3 was 9.34 ± 8.70 microgram/L (mcg/L), 35.97 ± 20.27 mcg/L, 31.68 ± 17.03 mcg/L, and 47.91 ± 15.47 mcg/L respectively. The mean serum titanium ion levels increased significantly at T1 (p= 0.009), T2 (p= 0.032), and T3 (p=0.00) interval. There was no significant difference between unilateral and bilateral groups.
Conclusion: Serum titanium ion continued to show increased levels till the last follow-up of 1 year. These initial serum titanium ion levels increase is due to the initial wear phase of the prosthesis which manifests over 1 year. Further studies with large sample sizes and long-term follow-ups are required to see the deleterious effect if any on the TMJ TJR.
The Effect of Student Fatigue on Teaching Quality: A Prospective Cohort Study Based on One University
Authors: xiaoqun Qin , ye Chen
Published: November 2022

Students' fatigue is a serious problem which affects the quality of teaching and the physical and mental health of students and must be paid attention to by the whole society and medical workers. This study was a prospective cohort study of fatigue related problems among computer science majors at a university. With reference to "Fatigue scale-14", combining with the learning characteristics of students in Chinese education system and applying the statistical principle, the "Fatigue Scale-18" was developed. In spring, summer, autumn and winter, 319 and 1276 college students were monitored for fatigue incidence, fatigue degree and gender differences. The incidence of fatigue was 64.97%, 70.73% in boys and 61.46% in girls. Psychological fatigue accounted for 79.33% and physiological fatigue accounted for 20.67%. The main causes of fatigue were analyzed. The influence of fatigue on teaching effect is empirically studied, and the influence of fatigue on learning of students with different learning foundations is studied. Effects of fatigue incidence in different climates on learning outcomes. Based on the above research, the quantitative relationship between academic performance and fatigue degree and gender was listed as follows: academic performance =90.208—2.906× fatigue grade—4.380× gender (boy 1, girl 2). Corresponding preventive measures are put forward in view of the causes of fatigue among college students, and it is suggested that the incidence of fatigue should be controlled below 30%; it is proposed that college education should pay attention to student fatigue, monitor students' fatigue status regularly or irregularly, and take corresponding preventive measures for the causes of fatigue. Reduce the impact of student fatigue on learning and health. It provides theoretical and empirical basis for preventing students' fatigue, improving teaching quality and ensuring students' health.
Endoscopic papillary balloon dilation can be safely performed in patients with dual antiplatelet therapy: A pilot study
Authors: Masuda Sakue , Jinushi Ryuhei , Koizumi Kazuya , Makazu Makomo , Nishino Takashi , Shionoya Kento , Kimura Karen , Sumida Chihiro , Kubota Jun , Ichita Chikamasa , Sasaki Akiko , Kobayashi Masahiro , Kako Makoto , Uojima Haruki , Sugitani Ayumu
Published: November 2022

Endoscopic papillary balloon dilation (EPBD)—a low-risk procedure for bleeding—has been suggested as an alternative to endoscopic sphincterotomy (EST) for papillary dilatation in patients undergoing choledocholithotomy and at a high risk of bleeding. Several guidelines recommend dual antiplatelet therapy (DAPT) be reduced to single antiplatelet therapy (SAPT) when EST is performed. However, there is no evidence that EPBD increases the risk of bleeding in patients receiving DAPT. Thus, we aimed to address this problem. We included 31 patients who underwent EPBD for CBD stones and received DAPT or SAPT prior to EPBD (9 in the DAPT group and 22 in the SAPT group) treated at our hospital from May 2014 to August 2022. The DAPT group included patients who underwent EPBD without antiplatelet therapy withdrawal or with a shorter withdrawal period than those recommended by the guidelines. In the DAPT group, one of the two antiplatelet agents used was thienopyridine. No bleeding was observed after EPBD in this study. We did not find any significant between-group differences in the change in hemoglobin levels and post-ERCP pancreatitis. Thus, we propose that EPBD does not increase the bleedingrisk in patients with DAPT.
Novel green fluorescent probe stem from carbon quantum dots for specific recognition of tyrosinase in serum and living cells
Authors: Wang Wenxian , Yang Dan , Zhou Yufei , Zhang Yuding , Guan Lijiao , Zhang Xianfen , Xue Weiming , Huang Saipeng
Published: November 2022

Tyrosinase (TYR), an important biomarker for melanoma, offered significant information early detection of melanoma and may decrease the likelihood of mortality. Therefore, this article constructed a highly sensitive and selective green fluorescent functionalized carbon quantum dots (TYR-CQDs) for tyrosinase (TYR) activity detection by one-step hydrothermal protocol utilizing catechol, citric acid and urea as precursors. The prepared TYR-CQDs illustrated excellent linear relationship and broad linear range with a low detection limit, which exhibited high accuracy and recovery in quantitative determination of TYR in human serum samples. Furthermore, the TYR-CQDs had successfully realized intracellular TYR detection owing to excellent biocompatibility, high anti-interference ability and good cellular imaging capability, suggesting the potential biomedical applications in early diagnosis of melanoma and other tyrosinase-related diseases.
A primate-specific (CCG) repeat in DISP2 is subject to natural selection in human and harbors unambiguous genotypes in late-onset neurocognitive disorder
Authors: Khamse S , Alizadeh S , SH Bernhart , Afshar H , Delbari A , Ohadi M
Published: November 2022

Intact blocks of (CCG)-repeats are among the top short tandem repeats (STRs), which have undergone natural selection. The above stems from the facts that these STRs are mutation hotspots for C to T truncating substitutions, and are predominantly enriched in the exons. The humanDISP2(dispatched RND transporter family member 2) has the highest level of expression in the brain, and contains a (CCG)-repeat at the interval between + 1 and + 60 of the transcription start site (ENST00000267889.5 DISP2-201), which ranks in the top 1 percent of (CCG) STRs in respect of length. Here we sequenced this STR in a sample of 448 Iranian individuals, consisting of late-onset NCDs (N = 203) and controls (N = 245). While the region spanning the (CCG)-repeat was highly mutated and contained several C to T transitions, which resulted in several (CCG)-residues, a 8-repeat of the (CCG)-STR was the predominantly abundant allele (frequency = 0.92) across the two groups. The overall distribution of alleles was not different between the two groups (p > 0.05). However, we detected four genotypes that belonged to the NCD group only (2% of the NCD genotypes, Mid-p = 0.02), and consisted of allele lengths that were not detected in the control group. We also found six genotypes that were detected in the control group only (2.5% of the control genotypes, Mid p = 0.01). While the group-specific genotypes formed a small percentage of the overall genotypes, they unveil an underappreciated feature, in which complex disorders such as late-onset NCDs may be linked with unambiguous genotypes.
High-harmonic generation in liquids with few-cycle pulses: effect of laser-pulse duration on the cut-off energy
Authors: Wörner Hans Jakob , Mondal Angana , Waser Benedikt , Balciunas Tadas , Yin Zhong , Neufeld Ofer , Tancogne-Dejean Nicolas , Rubio Angel
Published: November 2022

High-harmonic generation (HHG) in liquids is opening new opportunities for attosecond light sources and attosecond time-resolved studies of dynamics in the liquid phase. In gas-phase HHG, few-cycle pulses are routinely used to create isolated attosecond pulses and to extend the cut-off energy. Here, we study the properties of HHG in liquids, including water and several alcohols, by continuously tuning the pulse duration of a mid-infrared driver from the multi- to the sub-two-cycle regime. Similar to the gas phase, we observe the transition from discrete odd-order harmonics to continuous extreme-ultraviolet emission. However, the cut-off energy is shown to be entirely independent of the pulse duration. This observation is confirmed by ab-initio simulations of HHG in large clusters. Our results support the notion that the cut-off energy is a fundamental property of the liquid, independent of the driving-pulse properties. Combined with the recently reported wavelength-independence of the cutoff, these results confirm the direct sensitivity of HHG to the mean-free paths of slow electrons in liquids. Our results additionally imply that few-cycle mid-infrared laser pulses are suitable drivers for generating isolated attosecond pulses from liquids.
Nimbolide targets multiple signalling pathways to reduce neuroinflammation in BV2 microglia
Authors: Katola Folashade O , Olajide Olumayokun A
Published: November 2022

Nimbolide, a limonoid compound found in the neem plant, was investigated for effects on neuroinflammation in BV2 microglia activated with LPS. Cultured BV2 cells were stimulated with LPS (100 ng/ml) followed by treatment with nimbolide (125, 250 and 500 nM). Results showed that nimbolide caused significant reduction in the levels of TNFα, IL-6, IFNγ, NO/iNOS, PGE2/COX-2 in LPS-activated BV2 cells. Further experiments revealed that LPS-induced increased expression of phospho-p65 and phospho-IκBα proteins were reduced in the presence of nimbolide. Also, LPS-induced NF-κB acetylation, increased binding to consensus sites, and transactivation, as well as phosphorylation of p38 and JNK MAPKs were reduced by nimbolide. Reduction of cellular ROS generation by nimbolide was accompanied by reduction in gp91phox protein levels, while antioxidant effects were observed through elevation in protein levels of HO-1 and NQO-1. It was observed that treatment of BV2 microglia with nimbolide resulted in reduced levels of cytoplasmic Nrf2 which was accompanied by increased levels in the nucleus. Furthermore, treatment with this compound resulted in increased binding of Nrf2 to ARE consensus sites accompanied by enhanced ARE luciferase activity. Knockdown experiments revealed a loss of anti-inflammatory activity by nimbolide in cells transfected with Nrf2 siRNA. Treatment with nimbolide resulted in nuclear accumulation of SIRT-1, while siRNA knockdown of SIRT-1 resulted in reversal of anti-inflammatory activity of nimbolide. It is proposed that nimbolide reduces neuroinflammation in BV2 microglia through mechanisms resulting in dual inhibition of NF-κB and MAPK pathways. It is also proposed that activation of Nrf2 antioxidant as well as SIRT-1 deacetylation mechanisms may be contributing to its anti-inflammatory activity.
Epidemiological and phylogenetic characterization of unusual P[14] rotavirus strains in hospitalized children with acute gastroenteritis
Authors: Tatsi Elizabeth Barbara , Koukou Dimitra-Maria , Dellis Charilaos , Dourdouna Maria-Myrto , Michos Athanasios , Syriopoulou Vasiliki
Published: November 2022

Rotavirus A (RVA) is characterized by molecular and epidemiological diversity. To date, 42G and 58P RVA genotypes have been identified, some of which have zoonotic origin, like P[14]. This study aims at the epidemiological and molecular characterization of human P[14] RVA. Fecal samples from children ≤ 16 years with acute gastroenteritis (AGE), hospitalized during 01/2007-12/2021, were tested for RVA by chromatographic immunoassay. Demographic, clinical and laboratory data were recorded. Positive RVA samples were G and P typed performing Sanger sequencing. Phylogenetic analysis of P[14] was performed using the Mega X software. Sixty (1.36%) out of 4427 children with RVA AGE, were infected with an unusual G (G6/G8/G10) or P (P[6]/P[9]/P[10]/P[11]/P[14]) genotype. P[9] (27/60; 45%) was the most common unusual genotype followed by P[14] (11/60; 18.3%). Median age of children with P[14] was 37.8 months (IQR:17.6–77.1), 6/11 were males and 4/11 resided to rural areas. Their symptoms were diarrhea (9/11; 81.8%), vomiting (7/11; 63.6%), fever (7/11; 63.6%) and moderate dehydration (6/11; 54.5%). All children were unvaccinated for RVA. Seasonal peak of P[14] was during spring (91%). The combinations G8P[14] (45.5%), G6P[14] (36.4%) and G4P[14] (18.1%) were detected. Phylogenetic analysis showed potential evolutionary relationship of three human RVA P[14] with a fox strain from Croatia. These findings enhance the potential zoonotic origin of P[14] and the interspecies transmission between nondomestic animals and humans, which may lead to new RVA genotypes with unknown severity.
An integrated high-density genetic linkage map of tobacco (Nicotiana tabacum L.)
Authors: Shen Yang , Wu Chenglin , Cheng Junqi , Li Yapei , Lin Zhongxu , Cao Jinglin
Published: November 2022

Tobacco is an important non-food crop with many applications, which is widely cultivated worldwide. Genetic linkage maps play an important role in QTL mapping, gene targeting, etc. However, due to the narrow genetic background and large genome of tobacco, the research on the tobacco genetic map lags behind. At present, although several linkage maps have been constructed based on SSR (simple sequence repeat) markers, different SSR markers limit their widespread applications. In order to construct an integrated linkage map, we identified the redundancy of tobacco SSR markers, and constructed a high-quality genetic map by using the integration method based on four tobacco genetic maps. Finally, 20 redundant markers were found from 3,354 SSR markers, and the integrated high-density genetic linkage map (IHD) had a total of 3,377 loci with a length of 2,489.82 cM and a density of 1.36 locus/cM. The high-density integrated genetic map will have great application in the field of QTL mapping and marker-assisted breeding in tobacco.
Micro-RNA-93-5p and micro-RNA-3613-5p in exosomes released from vascular endothelial cells regulate medial arterial calcification in hemodialysis patients
Authors: Dongliang Zhang , Chen Fu , Chengai Wu , Ziye Chen
Published: November 2022

Objective:
To determine the underlying mechanism for how circulating exosomes with micro-RNA (Exo-miRNA) cargos promote medial arterial calcification (MAC) in maintenance hemodialysis (MHD) patients.
Methods and Results:
Plasma samples and fistular vascular tissues were collected from 12 MHD patients. Histological examinations and Exo-miRNA aberrant expression assays were conducted on the samples. The Exo-miRNAs in supernatants of human umbilical vein endothelial cells (HUVECs) treated with asymmetric dimethylarginine (ADMA) were compared with those in control HUVECs. Two similar miRNAs between patient plasma samples and cell culture supernatants, miR-93-5p and miR-3613-5p, were selected for cell culture experiments in vitro. Human aortic smooth muscle cells (HASMCs) were incubated with mimics of these miRNAs or cocultured with ADMA-treated HUVECs using a transwell system. Target proteins in the cell lysates were detected by western blotting. The histological examinations provided images of MAC Compared with control HASMCs, up-regulation of S100A11a, PI3K p110α, NF-κB, p38 MAPK, Rab11a, and F-actin was observed in the miR-93-5p mimic group, while up-regulation of Rab11a and F-actin was noted in the miR-3613-5p mimic group.
Conclusion:
MiRNA-93-5p and miRNA-3613-5p in exosomes released from vascular endothelial cells enter into vascular smooth muscle cells and regulate MAC in MHD patients.
Vulnerability of rice and maize yield in response to the climate and aerosol variability over India
Authors: Gupta Dilip Kumar , Pramanick Subhajit , Singh Abhay Kumar
Published: November 2022

The progressive accumulation of anthropogenic aerosol pollution alters the physical and chemical properties of the atmosphere, causes extreme variations in weather variables, and has severe negative repercussions on agriculture. The present study evaluates the time series analysis of crop production, crop cultivation area, weather, and aerosol variations over the time period of the years 1998 to 2019 for rice and maize crops in India. The impact of weather and aerosol variations is also evaluated on rice and maize yield. The increasing trend of rice and maize yield is found with a rate of 0.888 million tons/year and 0.561 million tons/year over the time period of 1998 to 2019, respectively, due to the use of more sophisticated agricultural practices, optimum fertiliser, and irrigation, climate-resilient varieties, etc. The time series increasing trend is observed for the average value of weather variables (TMAX, TMIN, rainfall, relative humidity) and aerosol optical depth (AOD). While the average value of surface soil moisture (SM) and a fraction of absorbed photosynthetically active radiation (FAPAR) is found to decresing the trend over the time period 1998 to 2019. The main findings of this study are that the rice and maize yield is reduced by about (2.55%, 2.92%, 2.778%, 4.84%, 2.90%, 2.82%) and (5.12%, 6.57%, 6.93%, 6.54%, 4.97%, and 5.84%) due to the unexceptional variations in weather variables, namely maximum and minimum temperature, rainfall, relative humidity, soil moisture, and FAPAR during the time period of 1998 to 2019, respectively. However, the rice and maize yields are reduced by 7.9% and 8.8%, respectively, due to the increment of aerosol pollution. Overall, the study brings out conclusive evidence of the negative impact on rice and maize yield due to the variability in weather/FAPAR/AOD.
A modified sequential kalman asynchronous track fusion based on sound propagation time
Authors: Sheng xueli , Wang Yan , Mu Mengfei , Song Lai , Wu Zeyi
Published: November 2022

Abstract： This paper presents a modified sequential kalman asynchronous track fusion method based on the sound propagation time. The proposed method deals with the asynchronous issue, which is caused by different acoustic signal propagation times, for the distributed underwater acoustic detection systems. When the bearing of the target is estimated by various sensors distributed system, we adopt the the effective sound velocity approach to calculate the sound propagation time from the target signals to the receving sensors respectively. We also update the tracked target information asynchronously for distributed detection system, using the multi-sensor asynchronous sampling information. The sequential kalman filtering algorithm is employed here to achieve the asynchronous track fusion. Simulation results demonstrate that the proposed algorithm can significantly improve the location accuracy compared with the original algorithm. The adaptability of the proposed method to the addition and deletion of the fusion sensor is proved robust and has a certain application value.
The influence of Surgical Apgar Score for clinical outcomes and complications in the patients treated with single- level posterior lumbar interbody fusion
Authors: Shinohara Kensuke , Yokoo Suguru , Yamane Kentaro , Takao Shinichiro , Nakahara Shinnosuke , Takeuchi Kazuhiro
Published: November 2022

Background.
The Surgical Apgar Score (SAS) has effectively prognosticated major complications in several surgeries including spine surgery. However, whether SAS can predict complications and patient reported outcomes after posterior lumbar interbody fusion (PLIF) has not been evaluated. The aim of this study was to analyze the relationship between SAS and clinical outcomes after PLIF.
Methods.
This research was retrospective clinical study. All patients who underwent a PLIF with singe-level, planned surgery from 2010 to 2022 were enrolled. Multilevel, revision, and unplanned PLIFs were excluded. Operative notes and anesthesia records were reviewed for estimated blood loss, lowest mean arterial pressure, and lowest heart rate. Japanese Orthopaedic Association (JOA) lumbar score and JOA improvement rate were recorded. Patients were divided into four groups by SAS (0–4, 5–6, 7–8, 9–10) and associated complications in each group were reviewed. A correlation coefficient between SAS and JOA improvement rate was also calculated. The receiver operating characteristic (ROC) analysis was used to evaluate the discriminatory power of SAS.
Results.
A total of 815 patients met study criteria. Major complications were reported for 33 patients. The incident rate of complications was 53.8% with SAS 0–4, 7% with SAS 5–6, 2.6% with SAS 7–8 and 0% with SAS 9–10, respectively. There were no reported deaths. Peripheral vascular disease (p = 0.01), moderate or severe renal disease (p = < 0.0001), diabetes with end organ damage (p = 0.01) and lymphoma (p = 0.02) were significantly more common in patients with major complications. The SAS and complications ROC reported an area under the curve of 0.73. The correlation coefficient between SAS and JOA improvement rate was weakly positive (r = 0.25).
Conclusion.
The data on this study shows SAS demonstrates good discriminatory power to predict post-operative complications. The relationship between SAS and JOA improvement rate is weakly positive.
Causal relationships between DNA methylation、Golgi membrane proteins、endoplasmic reticulum aminopeptidase and abnormal spermatozoa: a two sample Mendelian randomization study
Authors: Pang Lei , Ding Zijun , Chai Hongqiang , Li Fei , Wu Ming , Shuang Weibing
Published: November 2022

To explore the causal relationship between DNA methylation, Golgi membrane protein, endoplasmic reticulum aminopeptidase, ADP-ribose pyrophosphatase, mitochondrialon abnormal spermatozoa by two-sample Mendelian randomization (TSMR) method.Genetic loci closely related to DNA methylation PhenoAge acceleration, Golgi membrane protein 1, endoplasmic reticulum aminopeptidase 1, endoplasmic reticulum aminopeptidase 2, ADP-ribose pyrophosphatase, mitochondrial were selected as instrumental variables, andTSMR was performed by the inverse variance weighted method, MREgger regression, and weighted median method respectively. Sensitivity analysis was conducted to evaluate the robustness of the MR results.IVW showed that the DNA methylation PhenoAge acceleration(OR = 1.12, 95% CI: 1.01–1.23), Golgi membrane protein 1(OR = 1.22, 95% CI: 1.04–1.44), and endoplasmic reticulum aminopeptidase 2(OR = 1.13, 95% CI: 1.04–1.24) were associated with an increased risk of abnormal spermatozoa. However, there was no evidence of the association between ADP-ribose pyrophosphatase, mitochondrial (IVW OR = 1.25, 95% CI: 0.95–1.63), endoplasmic reticulum aminopeptidase 1 (IVW OR = 1.01, 95% CI: 0.90–1.12) and abnormal spermatozoa. Sensitivity analysis detected little evidence of pleiotropy in the current study.There is a positive causal relationship between DNA methylation PhenoAge acceleration, Golgi membrane protein 1, and endoplasmic reticulum aminopeptidase 2 on abnormal spermatozoa.
The therapeutic effect of larval saliva and hemolymph of Lucilia sericata on the treatment of Leishmania major lesion in BALB/c mice
Authors: Rahimi Sara , Rafinejad Javad , Akhavan Amir Ahmad , Ahmadkhaniha Reza , Bakhtiyari Mahmood , khamesipour Ali , Akbarzadeh Kamran
Published: November 2022

Background
Treatment of Cutaneous Leishmaniasis (CL) remains a major challenge for the public health and medical community. Natural compounds such as larval products of Lucilia sericata flies have Leishmanicidal effects against some species of Leishmania. The present study aimed at assessing the in vitro effects larval products against the promastigote and intracellular amastigote forms of Leishmania major. Also, the therapeutic effect of larval products on lesions induced by L. major infection was evaluated in BALB/c mice models.
Methods
The parasites and cells were treated with concentration of larval products ranging from 75-750ug/mL for 24-120h. Lesion progression and parasite load were investigated in the models for assessing the therapeutic effects of the products.
Results
The larval saliva and hemolymph showed highly cytotoxic effect against L. major promastigotes with IC50 values of 100.6 and 37.96ug/mL, respectively compared to Glucantime with 9.480ug/mL. Also, the results indicated that the larval products of L. sericata had greater cytotoxic effects on the promastigotes of L. major than on the macrophage cells (P < 0.05). Treatment with larval products decreased the infection rate and the number of amastigotes per host cell at all concentrations. The in vivo results showed a significantly (p < 0.0001) smaller lesion size in the groups of treated mice compared with the untreated control group. The average parasite burden groups of treated mice (1.81 ± 0.74, 1.03 ± 0.45 and 3.37 ± 0.41) were significantly lower (p = 0.0001) than untreated control group (6.72 ± 2.37), and was similar to the group treated with daily injection of Glucantime (1.77 ± 0.99).
Conclusion
The results suggested that L. sericata larval products are effective L. major in vivo and in vitro. However, more clinical trial studies are recommended to appraise the effects of these larval products in human subjects.
Fast Long-Term Tracking via Probabilistic Regression and Target Center Regression
Authors: Han Yongbo , Liang Yitao , Li Lei
Published: November 2022

The difficulty of long-term tracking mainly lies in the tracking failure caused by out-of-view, occlusion, and intra-class distractors. To deal with these difficulties, existing methods divide the tracking process into two parts, local tracking and global re-detection, and focus on designing robust distractor awareness mechanisms and global re-detection strategies. However, most state-of-the-art long-term trackers ignore the efficient design of the model and therefore may either be inefficiency or time-consuming. In this work, a real-time Long-term Tracking framework based on a local-global switching strategy is proposed, which utilizes Probabilistic regression and Target center regression to accelerate the long-term tracking process (named LTPT). To achieve reliable interaction between the local tracker and the global re-detector, LTPT introduces a probabilistic perspective to interpret the output of the local tracker. Additionally, this probabilistic regression approach benefits from the complementary use of attention mechanisms and deep cross-correlation to emphasize multiple hypotheses in the search region. To achieve fast re-detection, LTPT uses a rough target center regression to obtain a search region that may contain the target, and then refines the target position through a local tracker. Experiments on several datasets demonstrate that the method achieves comparable performance to the state-of-the-art algorithms.
Recurrence rates following ileo-colic resection in pediatric patients with Crohn’s disease
Authors: Dreznik Yael , Samuk Inbal , Shouval Dror S. , Paran Maya , Matar Manar , Shamir Raanan , Totah Moussa , Kravarusic Dragan
Published: November 2022

Background: Ileo-colic resection (ICR) is an important therapeutic option for Crohn’s disease (CD) patients. There is limited updated data of clinical and endoscopic post-operative recurrence (POR) in pediatric patients with CD for the long run. We aimed to determine recurrence rates following ICR over an extended period of time and asses its risk factors.
Methods: This is a single-center retrospective review of 35 patients with CD between the ages of 6 to 17.9 years who required ICR between 2003 and 2021at Schneider Children Medical Center of Israel. Medical charts were reviewed at different time-points post-ICR.
Results:
Clinical recurrence following ICR was demonstrated in only 11.4% and 28.6% (n=4, n=10) in the first two and five years- much lower rates than what was reported so far. We found no specific risk factor that correlated with clinical recurrence, although patients that were treated with early prophylaxis of anti TNF medications following ICR tend to have less recurrence.
Conclusions:
We found lower POR following ICR, especially in the first years after surgery- which can be attributed to close surveillance and early medical treatment. Such surveillance seem to improve recurrence rates in the first years following ICR.
Cryogenic Treatment to tailor the Interface of Impact Modified PET/PBT Blends
Authors: Pande Kavita , Chopra Swamini , Deshmukh Abhay , Kumar Anupama , Peshwe Dilip
Published: November 2022

Cryogenic treatment of polymers is a well-known area of research these days. Many reports confirm the fact that similar to metals, polymeric materials also undergo some structural changes which ultimately enhances mechanical properties like wear performance, tensile strength, impact strength, elongation, hardness, etc. The cryogenic treatment has proven an advantage for improving the properties of both virgin polymers and polymer micro-/nano-composites. The present study, first of its kind, reports the effect of cryogenic treatment on polymeric blends. An attempt is made to explore the effect of the treatment parameters, like temperature and time, on impact modified and unmodified PET/PBT blends. Similar to the polymers and composites, the blend systems studied here reflect significant improvement by ~ 50% in wear performance, ~ 16–19% in tensile strength and ~ 9% in impact strength after cryogenic treatment. Thus, after extensive analysis of the material properties, structure and thermal behavior, cryogenic treatment of PET/PBT blends, both impact modified and unmodified, is advocated as an effective tool for improving the performance.
CAPTAIN JOHN SMITH IN HUNGARY AND TRANSYLVANIA
Authors: Серегина А.Ю.
Published: November 2022

Английский путешественник Джон Смит прославился как основатель первого английского поселения в Новом Свете и исследователь Америки. Однако большую часть жизни он провел в Старом Свете. Предлагаемая читателю публикация представляет комментированный русский перевод глав 1–17 автобиографического памфлета Джона Смита, посвященного его юности (1593–1604). В то время Смит был солдатом и воевал во Франции, Нидерландах, Венгрии и Трансильвании. Центральную часть его повествования занимают события Долгой войны Габсбургов с Османской империей. В статье показано, что венгерские события стали основой представления Смита о себе как о христианском рыцаре, достойном дворянского звания.

The name of John Smith, an English traveler and pamphleteer, is usually associated with the propaganda of European exploration of the New World. However, his reputation of a soldier and a man of the world was based on his fighting in European military campaigns, and especially — those that took part in Hungary and Transylvania in the course of the Long Turkish War (1593–1606). In his autobiographical pamphlet, extracts from which are published in Russian translation here, John Smith focused the major part of the text on a relatively short period (1600–1603) that he spent in Central and Eastern Europe. It will be argued that his Hungarian experience provided a basis for Smith’s self-image of a Christian soldier worthy of noble status.
Modern breast cancer therapy: from tamoxifen to T-cell engineering
Authors: Shevchenko Yu. A. , Kuznetsova M. S. , Khristin A. A. , Sidorov S. V. , Sennikov S. V.
Published: November 2022

The purpose of the study was to conduct a systematic literature review of high-technology methods in breast cancer treatment. Material and methods. To select information sources, a global search was used using the Web of Science, Scopus, PubMed, and RSCI databases. The search included the analysis of metadata by keywords, and relevant publications were used for full-text search. The review used 55 publications from 2001 to 2021. Most of the articles were published over the past 7 years. Results. Modern literature data presented in this review prove that long-term studies based on histological and immunological features of tumor development are very important for improving survival in breast cancer. Clinical treatment protocols that were based primarily on the anatomical characteristics of the disease are now switching to the biological mechanisms underlying carcinogenesis. Drugs targeting estrogen receptors play an important role in systemic therapy and make it possible to correct the mechanisms responsible for endocrine resistance. Targeted therapy targeting the HER2 receptor, especially in an antibody-drug conjugate combination, has associated cytotoxic therapy with anti-HER2 antibodies. Modern methods of biological therapy and cell engineering make it possible to develop methods for treating triple-negative breast cancer based on the regulation of the microenvironment, mechanisms of repair, immunosuppression, and the creation of a target from a larger repertoire of both surface and intracellular antigens. Conclusion. Promising strategies based on the use of signaling and metabolic pathways, cell surface molecules, and cell engineering increase the effectiveness of treatment and improve the progression-free and overall survival in breast cancer patients.
The state of cancer care in Russia: cancer of the small intestine (C17). Survival of patients, median survival, survival of patients according to tumor localization, histological pattern and stage of the disease (Population study at the federal district level). Part II
Authors: Merabishvili V. M.
Published: November 2022

Background. Small intestine cancer is extremely rare cancer worldwide with an incidence of less than 1.0 per 100,000 population. In 2020, 1,711 cases of small intestine cancer were recorded in Russia, including 781 cases among the male population, and 930 among the female population. It should be noted that in Russia, despite the decrease in the total number of new cancer cases associated with the coronavirus epidemic, the number of patients with small intestine cancer increased by 4.14 % from 2019 to 2020. In the Northwestern Federal district of the Russian Federation, 216 patients with newly diagnosed small intestine cancer were registered in 2020, (29 more patients than in the previous year). The purpose of the study was to analyze the efficiency of small intestine cancer care provision based on of the database of the population cancer Registry (db pcr) of the Northwestern Federal district of the Russian Federation, with an assessment of one- and five-year survival rates. Material and methods. To calculate the survival rates for patients with small intestine cancer, we selected 1922 patients from the database of the pcr of the Northwestern Federal district of the Russian Federation, for the period from 2000 to 2018. Standard methods for calculating survival rates according to the eurocare program were used. Results. The one-year survival rate of small intestine cancer patients increased from 50.0 % to 61.1 % from 2000 to 2018, and the five-year survival rate remained almost unchanged. The relative one-year survival rate of patients was 2.0 % higher. The five-year survival rate for five-year cohorts indicates defects in staging of small intestine cancer rather than an improvement in patient care; although this rate increased from 31.5 to 32.9 %. The histological detail of small intestine cancer according to the icd-10 was investigated. Conclusion. The study confirmed the high mortality rates and modest survival benefits in survival rates in patients with small intestine cancer. Defects in the distribution of patients by disease stages were revealed. The most common histological types of small intestine cancer with calculations of patient survival were identified.
Pengaruh Latar Belakang Pendidikan terhadap Kompetensi Pedagogik Guru Taman Kanak-kanak di Kabupaten Bengkalis
Authors: Selian Nurhaida , Khodijah Siti
Published: November 2022

Penelitian ini bertujuan untuk mengetahui pengaruh latarbelakang pendidikan terhadap kompetensi pedagogik guru di TK  gugus 3 kenanga kecamatan bantan kabupaten bengkalis.  Hasil penilaian menunjukkan bahwa ada pengaruh latar belakang pendidikan terhadap  kompetensi pedagogik guru TK. Besarnya kontribusi latar belakang pendidikan terhadap kompetensi pedagogik guru TK sebesar 19,8% sisanya 80,2% dipengaruhi oleh faktor lain yang tidak dibahas dalam penelitian ini.  Dengan demikian analisis pengaruhnya adalah positif dan dibuktikan dengan nilai fhitung 7,657 f tabel4,16. Sehingga dapat disimpulkan bahwa Ha diterima dan Ho ditolak.
Simões Network 2 (SN2): A special model for special needing
Authors: Almeida Cardoso Dalton Humberto , Valério Patrícia
Published: November 2022

Functional Orthopedics appliances must follow strict rules of construction and respect the correct indications, to achieve the proposed goals. The approach must be individualized for each patient according to each needing. Dr Wilma Alexandre Simões created a network of appliances, with no similarity with other appliances created by other authors. Her appliances respect rigid scientific fundamentals and provide for the clinician special tools for solving different types of occlusopathies. Here we present some rules, developed, and scientifically supported by the authors, in order to optimize the use of one of Simões Network called SN2.
The interface between dental topography and functional appliances accessories
Authors: Almeida Cardoso Dalton Humberto , Valério Patrícia
Published: November 2022

Dental topography can be defined as the division of receptive fields that we have in the crown of the tooth. It is necessary that we understand this concept very well because it makes all the difference in clinical practice. We need to understand the receptive fields of the tooth, and we must use this knowledge when prescribing accessories for a functional appliance, understanding its function and where these accessories should touch to generate the expected effect. In this paper we will discuss the fundamentals of dental topography and show some accessories used on the therapeutic arsenal of Jaw Functional Orthopedics.
Cephalometric components and their implications in mandibular positioning
Authors: Rubio Terán Daniela , Rivera Pérez Iván Yamir , Núñez Hernández Elia Irene , Terán Alcocer Aidé
Published: November 2022

The mandibular position is determined by several physiological factors, which should be considered for diagnosis, therapy and stability. Sixty-eight lateral skull radiographs were taken and plotted to determine the relationship of the occlusal plane with the mandibular position among others. A Spearman statistical analysis was performed to determine the relationship between these variables. The occlusal plane showed a statistically significant correlation with mandibular position.
Methodology of Regional Strategising in Central Asia
Authors: Mirziyoeva Saida
Published: November 2022

Central Asia is currently undergoing some major transformations on its way to a new active sub-region with a wide system of international relations. The article features various strategies that the countries of Central Asia design for their socio-economic development. It introduces some objective prerequisites that shaped the idea of socio-economic integration of the peoples of Central Asia. Uzbekistan encourages this integration by creating and promoting a new image of Central Asia as a steadily developing and prosperous region with a shared economic and political environment. The author analyzed the approaches that the countries of Central Asia employ to develop and implement their own socio-economic strategies. Following the methodology of strategizing developed by Professor V.L. Kvint, the strategies are harmonized at various levels within the common Central Asian agenda. The current global transformation of the international order makes it difficult to make any reliable forecasts. However, Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, and Uzbekistan present a role model for other regions as they seek to come up with consolidated responses to the new challenges while using their potential and competitive advantages as part of regional and national strategies.
Location of an Industrial Enterprise: Strategic Analysis
Authors: Vlasyuk Lyudmila
Published: November 2022

The article deals with issues related to the development of a strategy for the location of an industrial enterprise. The location strategy is part of the corporate strategy, which defines its values and interests. A corporate location strategy is often linked to an entry strategy. The article introduces a strategic analysis of the factors that determine the location of an industrial enterprise, depending on the evaluation criteria and the industry specifics. The study is based on the theory of strategy and the methodology of strategizing developed by Professor V.L. Kvint. This approach helps to choose the location of an industrial enterprise, consider its global, national, and sectoral long-term development, and take into account its external and internal environment. The strategic analysis of placement factors and alternative options was based on an OTSW-analysis and special decision-making methods. The paper also reviews the relevance of location factors from the first theoretical works to modern empirical studies based on surveys of company managers and econometric analyses. The author designed a general algorithm for developing a strategy for locating an industrial enterprise. The theory of strategy and methodology of strategizing made it possible to neutralize the negative consequences of subjective decisions that still interfere with the location strategizing.