bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis and comparative assessment of ESIPT core containing lightfast fluorescent monoazo dyes
Authors: Ramugade Supriya H , Nethi Sekar Nagaiyan
Published: March 2022

Four fluorescent monoazo dyes were synthesized from “4-amino-2-(benzo[d]oxazol-2-yl) phenol” and “5-amino-2-(benzo[d]oxazol-2-yl) phenol.” The ESIPT core is incorporated in the dyes to enhance the lightfastness/photostability. These dispersed dyes were characterized by 1H NMR, FT-IR, mass, and CHN analysis. The various properties of fastness, XRD analysis, particle size analysis by comparing conventional method(C) versus nanoemulsion method (N) on polyester fabric with reduced liquor ratio were performed. Electrophilicity index was calculated and correlated with light fastness properties on polyester by both methods. Both methods observed higher electrophilicity index (6–7) and lightfastness properties (6–7) for all the dyes. The nanoemulsion dye "Z-2-(benzo [d] oxazol-2-yl) − 5 - (4-(diethylamino) – 2 - hydroxyphenyl) diazenyl) phenol" has the largest particle size distribution (55 nm). The HOMO-LUMO energy gap for the dye 6aA is the largest, as shown by density functional theory (DFT) investigations, further confirmed by its highest electrochemical values obtained from cyclic voltammetry.
Heat shock transcription factor 2 promotes mitophagy of intestinal epithelial cells through PARL/PINK1/Parkin pathway in ulcerative colitis
Authors: Liang Hao , Zhang Fengrui , Wang Wen , Zhao Wei , Zhou Jiao , Feng Yuran , Wu Jing , Li Maojuan , Bai Xinyu , Zeng Zhong , Niu Junkun , Miao Yinglei
Published: March 2022

Objective and backgroundThe overactivation of NLRP3 inflammasome is a critical factor for ulcerative colitis (UC). We found that heat shock transcription factor 2 (HSF2), which is highly expressed in UC, could inhibit the activation of NLRP3 inflammasome and reduce IL-1β in intestinal epithelial cells(IECs), but the mechanisms were still unclear. We aimed to elucidate the mechanisms of HSF2 inhibiting the activation of NLRP3 inflammasome of IECs.MethodsTEM was used to detect the number of damaged mitochondria and the level of mitophagy in intestinal mucosa of UC patients, healthy controls, mice and Caco-2 cells. ROS levels were detected by immunofluorescence or flow cytometry. RT-PCR, WB and immunohistochemistry were used to detect the level of PARL, PINK1 and Parkin.ResultsThe levels of mitophagy and ROS in intestinal mucosa of UC patients were positively correlated with disease activity. In IECs, after downregulation of HSF2, mitophagy reduced and number of damaged mitochondria and ROS increased, while overexpression of HSF2 showed opposite results. In addition, HSF2 might regulate mitophagy through PARL/PINK1/Parkin signaling pathway.ConclusionsHSF2 may promote mitophagy and decrease the level of mtROS through the PARL/PINK1/Parkin signaling pathway, which might be a potential mechanism of HSF2 inhibiting inflammation in UC.
Genome-wide mapping of oncogenic pathways and genetic modifiers of chemotherapy using a high-risk hepatoblastoma genetic model
Authors: Fang Jie , Singh Shivendra , Natarajan Sivaraman , Tillman Heather , Abuzaid Ahmed , Durbin Adam , Lee HaWon , Wu Qiong , Steele Jacob , Connelly Jon , Cheng Changde , Jin Hongjian , Chen Wenan , Fan Yiping , Pruett-Miller Shondra , Rehg Jerold , Emmons Joseph , Cairo Stefano , Wang Ruoning , Glazer Evan , Murphy Andrew , Chen Taosheng , Davidoff Andrew , Easton John , Chen Xiang , Yang Jun
Published: March 2022

A lack of relevant genetic models and cell lines hampers our understanding of hepatoblastoma pathogenesis and the development of new therapies for this neoplasm. We report a liver-specific MYC-driven hepatoblastoma murine model that faithfully recapitulates the pathological features of mixed fetal and embryonal hepatoblastoma, with transcriptomics resembling the high-risk gene signatures of the human disease. Single-cell RNA-sequencing and spatial transcriptomics identified distinct subpopulations of hepatoblastoma cells. After deriving cell lines from the mouse model, we mapped the cancer dependency genes using CRISPR-Cas9 screening and identified druggable targets shared with human hepatoblastoma (i.e., CDK7, CDK9, PRMT1, PRMT5). Our screen also revealed oncogenes and tumor suppressor genes in hepatoblastoma that engage multiple, druggable cancer signaling pathways. Chemotherapy is critical for human hepatoblastoma treatment. A genetic mapping of doxorubicin response by CRISPR-Cas9 screening identified modifiers whose loss-of-function synergizes with (e.g., PRKDC) or antagonizes (e.g., apoptosis genes) with the effect of chemotherapy. The combination of PRKDC inhibition and doxorubicin-based chemotherapy greatly enhances therapeutic efficacy. These studies have provided a useful set of resources including disease models suitable to identify and validate potential therapeutic targets in human high-risk hepatoblastoma.
Emotion Control Training Enhances Reappraisal Success Among Individuals With Reported ADHD Symptoms
Authors: Hamerman Revital , Cohen Noga
Published: March 2022

Previous research indicates that training individuals to recruit cognitive control before exposure to negative pictures can facilitate the propensity to use reappraisal and reappraisal success. Individuals with attention deficit hyperactivity disorder (ADHD) experience difficulties in cognitive control and emotion regulation, so they may especially benefit from such training. Individuals reporting high ADHD symptoms and controls were randomly assigned to one of two training conditions. In the high emotion control (H-EC) training condition, negative pictures were typically preceded by a stimulus that recruits cognitive control. In contrast, in the low emotion control (L-EC) training condition, negative pictures were typically preceded by a stimulus that does not recruit cognitive control. Participants were then asked to recall an adverse personal event and to reappraise the event. As predicted, instructed reappraisal was more effective in reducing negative mood in the H-EC training compared to the L-EC training. Furthermore, compared to controls, individuals with reported ADHD symptoms showed a greater propensity to use reappraisal after writing the event and a more considerable reduction in event significance and negativity following the instructed reappraisal assignment. We argue that employing cognitive control over emotional information has a causal role in reappraisal use and success among individuals with ADHD symptoms.
Diabetic foot infection caused by Burkholderia cepacia complex: Report of an unusual case and a scoping review
Authors: Leme Rodrigo Cuiabano Paes , Chaves Jéssica Reis Esteves , Gonçalves Luiz Carlos Soares , Alvim Leonardo César , de Almeida João Roberto Chaves , Renó Leonardo da Cruz
Published: March 2022

Burkholderia cepacia complex (BCC) is a widespread Gram-negative bacillus organized into more than 20 phylogenetically bacterial species. According to previous publications, BCC species are widely described pathogens in patients with cystic fibrosis (CF) but not in individuals with diabetes mellitus (DM). This study presents a case report of a diabetic foot infection (DFI) caused by BCC in a 42-year-old male patient hospitalized after an antibiotic treatment failure and the recommended drugs by the hospital. The patient improved after two surgical debridement procedures and a high-dose extended infusion (EI) of meropenem. The team of vascular surgeons and the infectologist worked fervently to solve the case. Finally, a scoping review was constructed to map the BCC infections in patients with DM.
Intimate partner violence and associated factors among reproductive age women in Liberia: Further analysis of recent Liberian demographic and health survey
Authors: Tsegaw Menen , Mulat Bezawit , Shitu Kegnie
Published: March 2022

Background: Intimate Partner Violence (IPV) is a major public health problem and a violation of women's human rights, almost one third of women aged 15-49 years who have been in a relationship have experienced to some form of physical and/or sexual violence by their intimate partner worldwide.Objective: The study aimed to assess intimate partner violence and associated factors among reproductive -age women in Liberia. Method: This study was based on a large community-based cross-sectional survey, Liberia Demographic Health Survey (LDHS), conducted From October 16, 2019, to February 12, 2020, in Liberia. The 2019–20 LDHS used a stratified two-stage cluster design. Multivariable logistic regression was used to identify independent intimate partner violence among reproductive age women in Liberia and to control confounders. Adjusted odds ratio and confidence interval (CI) were used to declare statistical significance in the final model. Those variables with p-value<0.05 was considered as statistically significant.Result: The overall prevalence of IPV was 44.74% (42.73, 46.77). Older age (AOR=0.86 (95%CI: 0.81, 0.91)), south central region (AOR=0.71(95%CI: 0.52, 0.96)), women’s primary education (AOR=1.28 (95%CI: 1.01, 1.63)), female household head (AOR=0.77 (95%CI: 0.61, 0.97)) ,husbands higher education (AOR=0.62(95%CI: 0.39, 0.99)) ,positive wife beating attitude (AOR=1.57 (95%CI: 1.29, 1.90)) ,husband drinks (AOR=2.59 (95%CI: 2.14, 3.15)) and Women’s decision making autonomy (AOR=0.75 (95%CI: 0.61, 0.93)) were significantly associated with IPV.Conclusion: The prevalence of IPV in Liberia was high. Socio-demographic characteristics of women, husbands education, sex of household head, having a positive attitude towards wife-beating, partner’s alcohol drinking habit and women empowerment was significantly associated with IPV in Liberia. Policymakers and program designers have to take into account those factors when they design interventions to reduce IPV in Liberia.
A novel splice site variant c.1183+1 G>C in DFNA5 causing autosomal dominant nonsyndromic hearing loss in a Chinese Family
Authors: Li Qiong , Wang Shujuan , Liang Pengfei , Li Wei , Wang Jian , Fan Bei , Yang Yang , An Xiaogang , Chen Jun , Zha Dingjun
Published: March 2022

BackgroundThe major clinical manifestation of autosomal dominant nonsyndromic hearing loss (ADNSHL) is bilateral, symmetrical, postlingual progressive sensorineural hearing loss, which begins with impairment at high frequencies and eventually progresses to hearing loss at all frequencies. Autosomal dominant deafness-5 (DFNA5) is a subtype of ADNSHL caused by a heterozygous variant in the gasdermin E (GSDME, also known as DFNA5) gene. MethodsTargeted genomic capture and sequencing were performed on the proband of a six-generation Chinese family with hearing loss. The co-segregation analysis between the hearing loss and the novel variant was analyzed by Sanger sequencing and pure-tone audiometry. The minigene splicing assay was performed to evaluate the potential effect of the variant on messenger RNA splicing in vitro. ResultsThe family exhibited autosomal dominant, progressive, postlingual, nonsyndromic sensorineural hearing loss, which was similar to the previously reported DFNA5 families. A novel heterozygous splice site variant in GSDME gene intron 8 was identified as “Likely pathogenic” according to the American College of Medical Genetics and Genomics (ACMG) guidelines, which co-segregated with the hearing loss phenotype of the family. The variant caused skipping of exon 8 in the mutant transcript, leading to the direct linking of exon 7 and 9.ConclusionsWe identified a novel GSDME splice site variant c.1183+1 G>C in an extended Chinese family, which led to the skipping of exon 8, through targeted genomic capture and sequencing and co-segregation analysis. The results extended the pathogenic variant spectrum of the GSDME gene, provided further support for the “gain-of-function” mechanism of DFNA5, and afforded a molecular interpretation for these patients with ADNSHL.
Monitoring The Land Surface Temperature And Its Correlation With NDVI Of Chiniot By Using GIS Technology And Remote Sensing
Authors: Yaseen Ahmed , Khan Aleeza
Published: March 2022

The unplanned and rapid increased in unban area have changed the atmospheric conditions and the non-urban area have less warm as compare to urban areas. In study area little portion of Chenab River include and the river bed contains the sand it shows high temperature value due to its specific heat energy. For this study Landsat-5(February2000), Landsat-7(February 2005,2010) and Landsat-8(Feb2015, Feb2020, Feb2022) acquired from USGS. The LST and NDVI correlation is negative and it is clearly seen that in the analysis of Landsat-8(OLI/TIRS) the Land Surface Temperature is increases 3.5408oC from 2015 to 2022. The analysis of Landsat-7(ETM+) the Land Surface Temperature is increases 3.3461oC from 2005 to 2010. In the Correlation analysis(LST&NDVI) the most of vegetation occurs between 11 oC to 28 oC.
The Application of Remote Sensing Technology in Forestry Carbon Sequestration
Authors: Tian Qimin
Published: March 2022

Forests absorb a great deal of carbon from the atmosphere every year and are strategically important for climate change mitigation. To better analyze the carbon sequestration value of forests, We established a carbon sequestration model. A net ecosystem productivity remote sensing map model was established based on the remote sensing idea of spatial informatics, which was used to measure the carbon sequestration capacity of forests. In addition, we measured the carbon sequestration of forests on each continent during the decade from 2003 to 2012, and compared the results to summarize the characteristics of good forest management. In order to better study the change of forest carbon sequestration over time and optimize the forest management plan, we selected the Greater Khingan Mountains in China as the research object. The Grey Prediction Model was established to predict the carbon sequestration model of the forest in the next 100 years. The results showed that Greater Khingan Mountains forest could sequestrate 167,996,510.3t/a carbon in the next 100 years.
The quality of life of children facing life limiting conditions and of their parents in Belgium: a cross sectional study
Authors: Friedel Marie , Aujoulat Isabelle , Brichard Bénédicte , Fonteyne Christine , Renard Marleen , Degryse Jean-Marie
Published: March 2022

Background: Paediatric palliative care (PPC) aims to improve the quality of life of children, however, this objective is rarely measured in clinical care. PPC is provided in Belgium through 6 transmural paediatric liaison teams (PLTs) ensuring continuity of care for children with life-limiting or life-threatening conditions (LLC/LTC). This study aims to measure the quality of life (QoL) of children with LLC/LTC monitored by PLT and the QoL of their parents.Methods: During interviews with children and parents, an original sociodemographic questionnaire, the Children Palliative Outcome Scale – version 2 (CPOS-2), the Fragebogen für Kinder und Jugendliche zur Erfassung der gesundheitsbezogenen Lebensqualität (KINDL) and the Quality of Life in Life-threatening Illness-Family Caregivers (QOLLTI-F) were completed by PLT members. Descriptive statistics were conducted on the sociodemographic data and the CPOS-2 and QOLLTI-F scores. The association between clinicians’ judgement of QoL and the scores on the QOLLTI-F and CPOS-2 was explored using Spearman’s correlation coefficients and boxplots. Pearson’s chi square test and Fisher’s exact test were used to investigate significant differences between scores. The results were discussed and interpreted with 6 PLTs.Results: Seventy-three children aged 1-18 years were included in the study. For items focused on emotional well-being, children reported that their QoL was higher than that of their parents. The QoL scores were not significantly associated with the severity of the child’s condition. Discrepancies were not found between health care professionals’ perceptions and parents’ perceptions of their QoL, as measured by the CPOS-2.Conclusion: This study provides, for the first time, an overview of the QoL of children and parents followed-up by PLT in Belgium.
Fully automatic landmarking of 2D photographs identifies novel genetic loci influencing facial features
Authors: Li Qing , Chen Jieyi , Faux Pierre , Bonfante Betty , Fuentes-Guajardo Macarena , Mendoza-Revilla Javier , Chacón-Duque Juan , Hurtado Malena , Villegas Valeria , Granja Vanessa , Jaramillo Claudia , Arias William , Barquera Rodrigo , Everardo-Martínez Paola , Sánchez-Quinto Mirsha , Gómez-Valdés Jorge , Villamil-Ramírez Hugo , de Cerqueira Caio C. Silva , Hünemeier Tábita , Ramallo Virginia , Wu Sijie , Du Siyuan , Gonzalez-José Rolando , Schüler-Faccini Lavinia , Bortolini Maria-Cátira , Acuña-Alonzo Victor , Canizales-Quinteros Samuel , Gallo Carla , Poletti Giovanni , Rojas Winston , Rothhammer Francisco , Navarro Nicolas , Wang Sijia , Adhikari Kaustubh , Ruiz-Linares Andrés
Published: March 2022

We report a genome-wide association study for facial features in > 6,000 Latin Americans. We placed 106 landmarks on 2D frontal photographs using the cloud service platform Face++. After Procrustes superposition, genome-wide association testing was performed for 301 inter-landmark distances. We detected nominally significant association (P-value < 5×10− 8) for 42 genome regions. Of these, 9 regions have been previously reported in GWAS of facial features. In follow-up analyses, we replicated 26 of the 33 novel regions (in East Asians or Europeans). The replicated regions include 1q32.3, 3q21.1, 8p11.21, 10p11.1, and 22q12.1, all comprising strong candidate genes involved in craniofacial development. Furthermore, the 1q32.3 region shows evidence of introgression from archaic humans. These results provide novel biological insights into facial variation and establish that automatic landmarking of standard 2D photographs is a simple and informative approach for the genetic analysis of facial variation, suitable for the rapid analysis of large population samples.
Low-density lipoprotein cholesterol is an independent risk factor associated with asymptomatic gallbladder stone disease in non-alcoholic fatty liver disease patients in northwest China: a case-control study
Authors: Lu zhengyang , Ganghua Yang , Lei Zhang , Fandi Meng , Yong Wan , Binwu Sheng , Qinhong Xu
Published: March 2022

Background
Non-Alcoholic fatty liver disease (NAFLD) has previously been reported to be related to the incidence of gallbladder stone disease (GSD). This study was developed to identify key factors associated with the incidence of new-onset GSD among patients with NAFLD.
Method
Data pertaining to 610 patients with new-onset asymptomatic GSD and 610 healthy subjects between the ages of 30 and 80 undergoing routine annual check-ups were retrospectively analyzed. Clinicopathological variables including height, weight, blood pressure, serum lipids indexes, fasting blood glucose were compared in these patients, and the risk of GSD was compared between patients with and without NAFLD. Moreover, associations between body mass index (BMI), blood lipids, and GSD were assessed through multivariate logistic regression analyses.
Results
GSD incidence was found to be significantly higher among NAFLD patients as compared to patients without NAFLD (P < 0.001). Among individuals with NAFLD, BMI, waist circumference, low-density lipoprotein cholesterol (LDL-c) levels, and triglyceride (TG) levels were all found to be significantly positively correlated with GSD (all P < 0.01), whereas a significant negative correlation between high-density lipoprotein (HDL-c) and GSD (P < 0.001) was observed in univariate analyses. In multivariate analyses, serum LDL-c was found to be significantly positively correlated with GSD incidence (P < 0.01), while BMI, serum TG, and HDL-c levels were negatively correlated with GSD incidence (all P < 0.01).
Conclusions
NAFLD is closely associated with the incidence of GSD and with related factors including obesity and dyslipidemia. These analyses suggest that serum LDL-c levels may be independently associated with the risk of GSD among individuals diagnosed with NAFLD, likely suggesting that improper drug treatment or pronounced reductions in TG levels may contribute to dysregulated cholesterol and fatty acid homeostasis in the context of hypertriglyceridemia in patients with NAFLD.
Causes and surgical management of choledocholithiasis after cholecystectomy
Authors: Xia HongTian , Xin XianLei , Zhang HangYu , Liang Bin , Yang Tao , Liu Yang , Wang Jing , Meng XiangFei
Published: March 2022

Background
The purpose of this study was to determine reasons for choledocholithiasis after cholecystectomy and the most effective treatment.
Materials and Methods
The records of patients with choledocholithiasis after cholecystectomy treated by the Department of Hepatobiliary Surgery (HS) or the Department of Gastroenterology (Gastro) at our hospital from 2007 to 2016 were retrospectively analyzed. Patients in the HS group received surgical exploration of common bile duct and lithotomy or exploration of the common bile duct, lithotomy, and cholangiojejunostomy. The Gastro group received exploration of common bile duct (endoscopic retrograde cholangiopancreatography) and lithotomy and endoscopic sphincterotomy. Complications and short- and long-term outcomes were compared.
Results
There were 86 patients in the HS group (mean age 43 ± 11 years) (56, exploration of the common bile duct and lithotomy; 30 exploration of the common bile duct, lithotomy, and biliary-enteric anastomosis) and 48 in the Gastro group (mean age 43 ± 13 years). In the HS group, 63% of patients had abnormal structure/dysfunction of the Oddis sphincter, and 91% had positive bile cultures. Exploration of the common bile duct, lithotomy and cholangiojejunostomy produced the highest excellent + good results rate (90%) and the lowest rate of recurrent choledocholithiasis (3%). Exploration of common bile duct and lithotomy (gastro group) was associated with the worst outcomes (excellent + good rate = 46%; recurrent choledocholithiasis = 46%).
Conclusion
Abnormal structure/dysfunction of the Oddis sphincter is the main cause of choledocholithiasis after cholecystectomy. Exploration of the common bile duct, lithotomy, and cholangiojejunostomy results in the best outcomes.
Orthologs of Human Circulating miRNAs Associated with Hepatocellular Carcinoma Are Elevated in Mouse Plasma Months before Tumour Detection
Authors: Ding Liang-Hao , Fallgren Christina M. , Yu Yongjia , McCarthy Maureen , Edmondson Elijah F. , Ullrich Robert L. , Weil Michael. M. , Story Michael D.
Published: March 2022

Research examining the potential for circulating miRNA as serve as markers for preneoplastic lesions or early-stage hepatocellular carcinoma (HCC) is hindered by the difficulties of obtaining samples from asymptomatic individuals. As a surrogate for human samples, we identified hub miRNAs in gene co-expression networks using HCC-bearing C3H mice. We confirmed 38 hub miRNAs as associated with HCC in F2 hybrid mice derived from radiogenic HCC susceptible and resistant founders. When compared to a panel of 12 circulating miRNAs associated with human HCC, two had no mouse ortholog and 7 of the remaining 10 miRNAs overlapped with the 38 mouse HCC hub miRNAs. Using small RNA sequencing data generated from serially collected plasma samples in F2 mice, we examined the temporal levels of these 7 circulating miRNAs and found that the levels of 4 human circulating markers, miR-122-5p, miR-100-5p, miR-34a-5p and miR-365-3p increased linearly as the time approaching HCC detection neared, suggesting a correlation of miRNA levels with oncogenic progression. Estimation of change points in the kinetics of the 4 circulating miRNAs suggested the changes started 17.5 to 6.8 months prior to HCC detection. These data establish these 4 circulating miRNAs as potential sentinels for preneoplastic lesions or early-stage HCC.
Employment Outcomes for Men and Women Following an Economic Downturn: Labour Underutilisation in Australia
Authors: Baum Scott , Mitchell William
Published: March 2022

In Australia, as elsewhere, there has been continuing interest in understanding questions regarding unequal employment opportunities. While aggregate patterns provide a useful overview, it is insightful to consider employment outcomes across segmented markets. One such segmented market is between men and women, where it is widely understood that labour market engagement opportunities will differ. This paper provides an investigation of these uneven labour market outcomes. It presents an analysis of labour underutilisation for men and women using panel data, taking account of both individual-level supply-side factors together with the strength of the local labour market (demand-side) and the performance of the broader macro-economic environment. The result is an analysis that accounts for the impact of changing macroeconomy, local labour market conditions, and men and women's employability assets.
A Comparison Study of Quantitative PCR and Conventional Culture-Based Methods for Microbial Water Quality
Authors: TAKCI Hatice Aysun MERCIMEK , KARACA Cevher
Published: March 2022

The goal of this study is to assess the microbial quality of water sources by using conventional culture-dependent microbiological methods and culture-independent molecular-based qPCR application for the monitoring of total bacteria, total coliform, and E. coli as an indicator of fecal contamination. In this experiment, a surface water sample was collected from Gaziantep, Turkey and the detection of microbial load and contamination was performed through gen-specific qPCR, plate count, and MPN quantification techniques. Total bacteria, total coliform, and E. coli levels using qPCR assay were significantly higher than that enumerated by culture-dependent microbiological methods in the water sample. Differences in the water pollution influenced the suitability of molecular method was clarified to the occurring viable but not cultivable (VBNC) bacteria and the measuring of low levels of DNA target by qPCR. The microbial quality of the Gaziantep Alleben water sample predicted by qPCR showed a positive correlation with traditional methods. These results stated that our real-time PCR approach can be suitable to estimate the relative abundances of uncultured and cultured indicator microorganisms affected by the water characteristics. The high level (100%) of resistance observed to clindamycin, erythromycin, penicillin G, tetracycline, and trimethoprim followed cefotaxime (62.5%), ceftazidime (50.0%), streptomycin (50.0%), ciprofloxacin (37.5%), and cefoperazone (25.0%) in a total of 8 strains. The high MAR index (0.33-0.67) of Gr negative strains indicated higher anthropogenic activity such as discharge points of industrial and domestic wastewaters which can contribute to microbiological contamination in surface waters.
Association of rs4711998 of IL-17A, rs2275913 of IL-17A and rs763780 IL-17F gene polymorphisms with vitiligo in a Mexican population
Authors: Ocampo-Candiani Jorge , Salazar Natalia Aranza Zapata , Kubelis-López David Emmanuel , Salinas-Santander Mauricio Andrés , Sánchez-Domínguez Celia Nohemí , Xolalpa-Rosales Ana Cecilia , Gómez-Galindo Marely Eugenia
Published: March 2022

Vitiligo is the most common depigmenting disease characterized by achromic macules due to selective loss of melanocytes. The pathogenesis remains poorly elucidated, and multiple hypotheses exists regarding its pathogenesis. Evidence suggests that stress on melanocytes can result in activation of the immune system, and involvement of both activated cluster of differentiation (CD8+) cytotoxic and CD4 + T-cells in the dysfunction, depigmentation, and apoptosis of melanocytes. Recent studies show that the interleukin 17 (IL-17) axis plays a central role in the pathogenesis of the disease. IL-17 is an important regulatory effector cytokine in this pathway. The aim of this study was to evaluate the association of IL-17A rs4711998 (-832A/G), IL-17A rs2275913 (-197G/A), and IL-17F rs763780 (7488A/G) with vitiligo in a Northeastern Mexican population. This study was a case-control study and included 116 patients with vitiligo and 116 control subjects. Genotype characterization of IL-17A rs4711998 (-832A/G), IL-17A rs2275913 (-197G/A), and IL-17F rs763780 (7488A/G) was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. A P ≤ 0.05 was considered significant. It was observed that the combination of the genotypes GG/GA for IL-17F rs763780 (7488A/G) were associated with an increased risk for the development of vitiligo (OR = 2.0943, 95% Cl 1.2375–3.5445, P = 0.0056). Regarding IL-17A genotyping rs4711998 (-832A/G) and IL-17A rs2275913 (-197G/A), no association with vitiligo development was found. In conclusion, the SNP rs763780 in the IL-17F gene appears to be a risk factor for vitiligo development in this Mexican population.
A Serotonin Story in a Toxicological Model of Parkinsonism: Real Time, In Vivo, Chemical Investigations in Mice
Authors: Buchanan Anna Marie , Mena Sergio , Choukari Iman , Vasa Aditya , Crawford Jesseca , Fadel Jim , Reagan Lawrence , Hashemi Parastoo
Published: March 2022

Loss of midbrain dopaminergic neurons is well characterized to underlie the motor symptoms of Parkinson’s Disease (PD). There are, however, a host of non-motor, and often devastating PD symptoms and less is known about how these symptoms are mediated by neurotransmitters. Serotonin neurochemistry is linked to various aspects of the disease such as PD-associated depression, levodopa (L-Dopa) induced depression and L-Dopa induced dyskinesia (LID). Depression and LID significantly reduce quality of life for PD patients so understanding serotonin neurotransmission in PD models is of great merit for reducing these symptoms. Here we use niche, fast electrochemistry paired with mathematical modeling and machine learning to, for the first time, robustly evaluate serotonin in vivo in real time in a toxicological model of Parkinsonism, 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP). Mice treated with an acute MPTP paradigm had lower concentrations of in vivo, evoked and ambient serotonin in the hippocampus, consistent with the clinical comorbidity of depression with PD. These mice did not chemically respond to an SSRI, as strongly as did control animals, following the clinical literature showing that antidepressant success during PD is highly variable. Following levodopa (L-DOPA) administration, using a novel machine learning analysis tool, we see a dynamic shift from evoked serotonin release to dopamine release. We hypothesize that this finding shows, in real time, that serotonergic neurons uptake L-DOPA and produce dopamine at the expense of serotonin, supporting the significant clinical correlation between L-DOPA and depression. Finally, we find that this post L-DOPA dopamine release is less regulated, staying in the synapse for longer. This finding, perhaps to lack of autoreceptor control, may underlie the clinical presentation of LIDS. For the first time, this work shows the chemical intricacies of serotonin and how this modulator underlies several important non-motor deficits of PD, opening up new avenues of study for ultimately alleviating these symptoms.
Acanthomatous Ameloblastoma: A Rare Presentation
Authors: Hathila R N , Shah Pinal C , Patel Archana V , Patel Kuntal , Tejani Nilam J
Published: March 2022

Ameloblastoma is a slow growing, locally aggressive neoplasm of enamel organ type tissue with a high propensity for recurrence. They occur either in maxilla or mandible at nearly any age but most frequently are discovered as a painless expansion in the mandible of patients in their 20s – 40s with equal frequencies in male and female. Histopathologically the follicular and plexiform patterns are the most common. When extensive metaplasia associated with keratin formation occurs in central portions of the epithelial islands of follicular ameloblastoma, the term Acanthomatous is applied. Here we present a case of Acanthomatous ameloblastoma in a 40 years old female patient.
O INVESTIMENTO NO “POTENCIAL DOS JOVENS” COMO UMA TECNOLOGIA BIOPOLÍTICA: REPENSANDO O PROTAGONISMO JUVENIL NO CONTEXTO DO “NOVO ENSINO MÉDIO”
Authors: Goulart Marcos Vinicius da Silva , Traversini Clarice Salete
Published: March 2022

O protagonismo juvenil ganha uma fundamentação teórica no Brasil com o trabalho do pedagogo Antonio Carlos Gomes da Costa na década de 1990, a partir da experiência de trabalho com jovens em organizações não-governamentais. Esse protagonismo é inspirado pelo que ficou conhecido como Relatório Jacques Delors, que enuncia os quatro pilares de uma “Educação para o século XXI”, a saber, aprender a ser, aprender a fazer, aprender a aprender e aprender a conviver. Com esse protagonismo o jovem formaria sua autonomia a partir de uma experiência mais voltada às suas próprias competências e habilidades. A partir de uma análise documental inspirada pelo trabalho de Foucault, buscamos analisar o discurso do protagonismo juvenil em conexão com a releitura que o filósofo desenvolve da noção de capital humano. Argumentamos neste texto que o protagonismo juvenil, para além de estar ligado a uma estratégia de investimento no “potencial da juventude” para o desenvolvimento social de uma população, também é uma estratégia biopolítica de formação de uma cultura empreendedora com vistas ao desenvolvimento econômico e social do país.
SABERES DOCENTES NA FORMAÇÃO INICIAL DE PROFESSORES DE QUÍMICA: NOVAS COMPREENSÕES À LUZ DA ANÁLISE TEXTUAL DISCURSIVA
Authors: De Lorena Stanzani Enio , Dias Broietti Fabiele Cristiane , Meneghello Passos Marinez
Published: March 2022

No presente artigo apresentamos os desdobramentos de um estudo sobre os Saberes Docentes (SD) mobilizados por futuros professores de Química, enfatizando as etapas, os resultados e as novas compreensões possibilitadas a partir de um movimento analítico fundamentado nos pressupostos da Análise Textual Discursiva (ATD). Nessa perspectiva, os relatos dos licenciandos coletados durante o processo de pesquisa foram analisados a partir de categorias construídas com base na tipologia dos SD proposta por Tardif. Desse processo de análise, dada a amplitude das categorias, emergiram subcategorias que nos possibilitaram evidenciar, de maneira mais pontual, quais aspectos os licenciandos mobilizaram ao pensar no planejamento e na ação docente em sala de aula, bem como as lacunas presentes nesse processo, relacionadas ao domínio dos conceitos científicos, à mediação pedagógica dos conhecimentos, à elaboração e execução dos planejamentos na realidade concreta das escolas e ao tempo de prática implicado para a reelaboração desses saberes. Tais considerações conclusivas apontam caminhos norteadores para os professores formadores na construção de espaços formativos que realmente deem conta da complexidade da prática docente, visando contribuir com o movimento de profissionalização defendido pelas pesquisas da área com enfoque na formação de professores.
Diseño y adaptación del serious game basado en el perfil del jugador del estudiante
Authors: García Lázaro Desiré , Sánchez Sánchez Felix
Published: March 2022

La tecnología educativa cobra especial importancia en las aulas, y sobre todo en el ciclo formativo de Formación Profesional donde la innovación parece que tarda en llegar. El trabajo que se presenta demuestra cómo la incorporación de un serious game en el proceso de enseñanza y aprendizaje tiene una repercusión positiva y directa sobre los resultados de aprendizaje. La puesta en práctica se realiza en un aula de grado medio de FP con una alta tasa de fracaso académico. Participan un total de 20 alumnos pertenecientes al curso académico 2019/2020. La metodología empleada es el Aprendizaje Basado en el Juego con la identificación del tipo de jugador que es cada alumno. Los resultados presentan un aumento de la motivación e implicación del alumnado y una mejora en el rendimiento académico.
Hybrid MAPbI3 Perovskite Growth Mechanism from Irregular Particles to Cuboid and Hopper-Type Morphologies
Authors: Bonadio Ariany , Souza Jose
Published: March 2022

Methylammonium lead iodide (MAPbI3), used as light-harvesting layer in solar cell devices, has attracted great attention from scientific community due to its excellent photovoltaic performance. In this work, we have shown a comprehensive study on the hybrid organic-inorganic MAPbI3 perovskite growth mechanism from irregular small particles to cuboid shape followed by hoppertype morphology through a systematically change on both the temperature and lead source concentration during the solution synthesis process. Indeed, we have observed very interesting hopper growth resulting in a pyramid-hollow like morphology. We have explained the morphology evolution by taking into account thermodynamics growth, which compete with kinetics growth under nonequilibrium conditions.
Exploring the potential for rainwater use for the urban poor in Bangladesh
Authors: Hasan Md Abid , Irfanullah Haseeb Md.
Published: March 2022

Under the climate crisis and rapid urbanisation, rainwater harvesting (RWH) is more pertinent than ever. In Bangladesh, the full potential of rainwater use, especially for the urban poor, has not been explored. This study reviewed the recently-executed national plans to assess the RWH provisions, explored literature to map RWH practice, showed the alignment between the plans and the practices, and assessed the provision of RWH in the currently-implemented plans, with a particular focus on the urban poor. Although the past plans recognised RWH, the study found that these did not emphasise the implementation process nor offer provisions for the urban poor. RWH was, however, widely practised in the urban, peri-urban, and rural parts of the country by utilising different catchment structures. Building on the experiences of other countries, the study assessed the feasibility of RWH for the urban poor to meet their sanitation and hygiene needs under the changing climate. The study concluded that a detailed ‘Rainwater Harvesting and Management Plan’ supporting the adoption and promotion of community-led models to mitigate RWH-related challenges in the urban slums and integrating multi-storied buildings to harvest rainwater for the poor neighbourhoods could ensure efficient and equitable rainwater management for the urban poor.
Seasonal variation of drinking water quality in urban water bodies (UWBs) of Chittagong Metropolitan City, Bangladesh: implications of higher water quality index (WQI) for the urban environment
Authors: Molla Morshed Hossan , Chowdhury Mohammad Abu Taiyeb , Bhuiyan Md. Habibur Rahman , Das Suman , Morshed A. J. M. , Das Jewel , Islam Saiful
Published: March 2022

This is an empirical study on small urban water bodies in Chittagong Metropolitan City, Bangladesh. The ultimate objective was to explore the alternative source of fresh water supply for the city dwellers–the urban poor. To determine the level of drinking water, a suitability analysis was performed in conjunction with the construction and calculation of a Water Quality Index (WQI) for two distinct seasons: Rainy and Winter. The IBM Statistical Package for the Social Sciences (SPSS) Statistics version: 20 and CAP: version: 5.0.0.465 was used as a means to an end. The study reveals that water quality in sampled UWBs of CMC was found unsuitable (WQI value 237.11) for drinking in the Rainy season and very poor (WQI value 99.62) in the Winter. The eight (8) parameters that crossed the maximum permissible limit in the Rainy and Winter seasons include electrical conductivity (EC), biological oxygen demand (BOD), chemical oxygen demand (COD), turbidity and nitrate. The two biological parameters, i.e. total coliform (TC) and fecal coliform (FC) that stood alone in crossing the admissible limit, detected measured values 1100+ MPN- 100 mL−1 in Rainy and Winter seasons, against unit recommended value 50. Awareness building on water pollutants in both public and private sectors is required to improve public health service delivery.
Antipyretic Medicinal Plants, Phytocompounds, and Green Nanoparticles:
An Updated Review
Authors: Sharma Rohit , Janmeda Pracheta , Chaudhary Priya , Rawat Sonam
Published: March 2022

Pyrexia itself is not a terminal condition. Basically, it occurs with mild to serious diseases
affecting alarge population of the world. Other than a high body temperature, pyrexia is accompanied
by several sickness behaviors, changes in physiological and metabolic characteristics of the
body system, and alterations in the immune responses. Various allopathic drugs are available to treat
pyrexia by targeting the symptom or the pathogen itself. Drug-resistance has made control and
treatment of vectors more difficult. However, many marginal people are obligated to utilize locally
available medicinal plants for the treatment of various diseases due to limited access to synthetic
drugs. Developments in the field of nanotechnology and phytochemical research towards the discovery
of new antimicrobial agents have also drawn the interest of researchers towards the synthesis of
green nanoparticles from plant extracts due to their several benefits over the other methods. Thus,
the present report discusses the use of ethnomedicinal plants, phytocompounds, and the application
of green nanoparticles synthesized from plant extracts to treat pyrexia.
Pediatric Takayasu Arteritis: A Review of the Literature
Authors: Kasapcopur Ozgur , Haslak Fatih , Yildiz Mehmet , Sahin Sezgin , Adrovic Amra , Barut Kenan
Published: March 2022

Takayasu arteritis (TA) is the third most common vasculitis of childhood and is extreme-ly rare. It is mainly characterized by chronic, autoimmune, and granulomatous inflammation of the aorta and its major branches. Women under 40 years of age are mostly affected. It occurs for the first time in childhood in about 30% of affected individuals. Initially, it presents nonspecific consti-tutional findings. Since there is no specific laboratory finding, diagnosis is challenging. The gold standard imaging method for diagnosis is conventional angiography. Delay in diagnosis can cause devastating consequences. Therefore, in cases presenting with nonspecific findings, with hyperten-sion and high acute phase reactants, the diagnosis should be suspected and confirmed with appro-priate imaging method, and treatment should be started immediately. Immunosuppressive agents are the mainstay of the treatment. Biological agents are successful in refractory cases, and endovascular revascularization methods are used in the treatment of complications.
A Case of Eosinophilic Granulomatosis with Polyangiitis Presenting with
Mononeuritis Multiplex
Authors: Alam Md. Adnanul , Hossain Md. Ismail , khan Abed Hussain , Arafat Shohael Mahmud
Published: March 2022

Background:

Eosinophilic granulomatosis with polyangiitis (EGPA), also known as Churg Strauss syndrome, is an uncommon vasculitis associated with antineutrophil cytoplasmic an-tibody (ANCA). The hallmarks of the disease are asthma, eosinophilia, and systemic vasculitis with varying degrees of neurological, cutaneous, cardiac, gastrointestinal, and renal involvement. Diag-nosis is often difficult since the symptoms are diverse, and a number of differentials need to be ex-cluded.

Case presentation:

In this report, we describe a 60-year-old patient who presented with mononeuri-tis multiplex and a painful skin rash. A history of late-onset asthma, which was poorly controlled, led us to suspect EGPA. Laboratory data showed leukocytosis, eosinophilia (>10%), elevated ESR, CRP, and IgE, normal chest Xray, positive rheumatoid factor (RA), perinuclear anti-neutrophil cy-toplasmic antibody (p-ANCA), and evidence of extravascular eosinophils in histopathology report of skin biopsy. She was treated with methylprednisolone and cyclophosphamide pulse therapy with a satisfactory response.

Conclusion:

Diagnosis of EGPA requires a combination of clinical and histopathological findings to meet the diagnostic criteria. A history of poorly controlled or late-onset asthma may guide us to the diagnosis that is frequently overlooked. Due to the wide heterogeneity of EGPA patients' pheno-types, sharp, professional judgment is needed for early disease detection and treatment in order to avoid irreversible changes and poor outcomes.
Preconception Care: Intention to Action- An Intervention Based on the
Planned Behavior Theory for Maternal and Neonatal Health: A Randomized
Clinical Trial
Authors: Aziz Maryam Valayi , Vakilian Katayon , Khorsandi Mahboobeh , Ranjbaran Mehdi
Published: March 2022

Background and Objectives:

Preconception care involves health promotion, screening,
and interventions for women and men to reduce the risk factors with the potential effect on their future
pregnancies; the present research is aimed at investigating the effect of planned behavior theory
on the knowledge, attitude, subjective norms, perceived behavior, self-control, intention, and practices
of preconception care.

Methods:

This is a controlled randomized trial study to examine the impacts on the intentions and
conductance of the preconception care among the women referring to a marriage consulting center.
To this end, 110 women referring to the marriage counseling centers of Asad Abad city in Iran were
selected and then divided into two groups. The educational sessions were held twice a week in four
60-minute sessions for the intervention group, while the control group received no intervention.
Wilcoxon and Mann-Whitney and ϰ2 were employed for data analysis at the significance level of
0.05. The reliability of the tools was evaluated using Cronbach Alpha.

Findings:

The results indicated a significant difference between the two groups in terms of awareness
(14.27 ± 1.53 vs. 5.32 ± 2.95), attitudes (45.89 ± 3.52 vs. 36.36 ± 6.77), subjective norms
(30.01 ± 3.34 vs. 26.38 ± 4.10), and perceived behavioral control (23.10 ± 2.06 vs. 18.87 ± 4.61) for
the intervention and control groups, respectively (P = 0.001). Also, 89% of the intervention group
(vs. 4% of the controls) tried to make the record for preconception care.

Conclusion:

The theory of planned behavior can effectively improve awareness, attitude, and perceived
behavioral control in women. This theory also improved the behavioral intention of women
toward preconception care. The midwives and physicians are recommended to use this educational
method in health centers to motivate the women to take preconception counseling with the help of
health providers.

Trial registration:

This study was registered in the Iranian registry of clinical trials with the code
IRCT2015121425529N1 in 2016.04.17