bluuebunny/crossref_metadata_2025 huggingface dataset:
PERSEPSI MAHASISWA BAHASA ASING TERAPAN UNIVERSITAS DIPONEGORO MENGENAI PJJ DARING
Authors: Kamah Audrey Sophia Rekka , Febrianti Yulia , Maulida Wildania Gadis , Alfarisy Fitri
Published: June 2022

Indonesia has implemented number of regulations in response to COVID-19, one of which is the temporary suspension of crowd-attracting activities, including in the education sector. It also effected to the learning process transition from offline to online learning system. The purpose of this research was to ascertain Applied Foreign Language students' perceptions of online learning. This study collects data through descriptive quantitative methods as main data, study literature as secondary data, and survey as data collecting method. The results indicated that some students of Applied Foreign Languages believed that online learning was still ineffective in its application, owing to a number of impediments, including unstable networks, a lack of comprehension of the provided material, a lack of space and time, and an unsupportive learning environment. Therefore, this research is supposed to serve as a tool for related parties in evaluating and seeking solutions over the issues that occured
Testing the Two-Step Model of Marketing Krishna Consciousness in Europe
Authors: Bence-Kiss Krisztina
Published: June 2022

Krishna Consciousness is a new religious movement, which has gained higher and higher importance in the past decades in Europe. During multiple researches between 2013 and 2020 (Bence, 2014; Bence-Kiss, 2020) a two-step model was identified, in which Krishna-conscious communities of Europe attract people outside the religion as tourists to rural communities in the different countries, where they gain more knowledge about the religion in informal, non-pressing ways. The Transtheoretical Model of Behavior Change (TTM) (Prochaska & DiClemente, 1983) may be applied to analyze the behavior through which people transform from tourists to actual members of the religious community via a set of behavior changes. The objective of this study was to test the applicability of this concept previously found (Bence-Kiss, 2020) on two countries, which have not been part of the initial research: France and Italy. Online content analysis, observations and in-depth interviews were applied to identify the general structure of the religious community in each countries; and the results were compared to the previous findings, confirming that the two-step model and TTM may also be applied to analyze the involvement into Krishna Consciousness in these countries as well.
Health Awareness Toward Dairy Functional Foods in Hungary
Authors: Mohammad Mohammad
Published: June 2022

Recently, the development of functional food products has played an essential role in healthy behavior. The increase in knowledge of consumers spurs concerns about health and how diet directly impacts health status. Functional foods are a new type of product that provide consumers with improvements in targeted physiological functions and a healthy lifestyle. Twenty-first-century consumers are seeking foods that have beneficial effects on the body beyond the conventional known nutritional role of the food. However, many consumers are still unclear about what functionality a given represents and the intended purpose of a given food product. In the Hungarian dairy sector, most dairy products stand out as the largest segment of functional foods giving the diversity of Hungarian’s functional dairy market. Several studies have looked at the trends of individual perception and consumer behavior regarding new foods in the market and their functional role in health and disease. The objective of this review is to highlight how health awareness influences the acceptance and consumption of dairy functional foods in Hungary.
A Study on the Development of Educational Content for Korean Studies based on Minjung Culture
Authors:   The Korean Society of Culture and Convergence, Cha Bongjun
Published: June 2022

The development of Korean studies educational content in various academic fields is necessary for considering the ever-increasing demand for Korean studies. In response to this reality, this study proposes the need to utilize the elements of minjung(people) culture, which form the basis of Korean culture, as content for developing practical Korean studies educational content to be provided to overseas Korean studies learners. In other words, developing a wide range of elements related to the concept of minjung culture in history, literature, language, ideology, and religion as educational content for Korean studies will be possible to approach the diverse needs of consumers of Korean studies more deeply. To this end, this study examines the necessity and methodology of educational content for Korean studies using the characteristics of the people(minjung) inherent in language and culture. Also, this study delves into the development of interdisciplinary educational content based on history and ideas. Analysis of the current status of Korean studies and the current demand for Korean studies were also briefly reviewed. In addition, as an example of the development of Korean educational content based on minjung culture, a lecture structure was presented for the subject ‘Minjung culture and Korean proverbs.’ In particular, this course can be helpful as a model for developing K-MOOC courses for overseas Korean studies consumers by developing a structure on the premise of MOOC courses.
Community Awareness and Place of Practice of Personality Education for Ego Identity
Authors:   The Korean Society of Culture and Convergence, Choi Hyeran
Published: June 2022

The spread of COVID-19, a new infectious disease with strong contagious and contagious power, which occurred in a defenseless state without a cure or vaccine at the end of December 2019, drove the world into anxiety and fear. The WHO(World Health Organization) has officially declared COVID-19 a pandemic. In order to reduce the spread of the virus and the risk of group infection, the Korean government had implemented various quarantine policies such as mask recommendations, high-intensity social distancing, group restrictions, diagnostic tests for symptomatic patients, and self-isolation. However, there were difficulties in quarantine activities due to people who did not recognize the seriousness of the infection and acted self-centered. These behaviors can be said to be related to personality. Humanity means the right mind, which is the character of a human being, and the right mind can be cultivated through character education. Ultimately, through character education, one can break free from self-centeredness and form a sense of community with others. In this study, as a new personality education method, a venue for the establishment of self-identity and community consciousness was proposed for college students. Establishing a self-identity is very important for the formation of a holistic personality because it is to maintain self-consciousness and meaning consistently. In addition, I think that the establishment of self-identity will affect the cultivation of a sense of community to live well with others. Therefore, in this study, we tried to propose a method to cultivate self-identity and sense of community by using cooperative learning teaching method.
The Effect of e-learning Readiness on Perceived Learner’s Achievements in Online Korean Language Classes
Authors:   The Korean Society of Culture and Convergence, Cho Yunkyoung
Published: June 2022

The purpose of this study was to analyze the factor affecting their relationships with e-learning readiness and academic achievement of Korean learners in online Korean language classes. To do this, e-learning readiness consisting of five areas and 22 question such as self-directed learning, online relationship, video learning contents, collaboration learning and success factor for class were used to investigate tool. In this study, e-learning readiness survey response data were analyze for 66 students, the correlation and multiple regression analysis of Pearson was conducted. Main results are as follows. First, E-learning readiness survey results across the five areas of Korean learner recognized an average 3.768 and the majority of learners have a more normal level competence. Second, the results of between E-learning readiness and learner’s achievement had correlation with academic achievement. Third, the result that E-learning readiness factor of Korean learner does the effect on on-line relationship and collaboration learning. The result of this study can be utilized in an effort to develop high-qualified on-line course in Korean education, and it is suggested that non-face-to-face class will be establish as a new course for learners, not only offline class.
Korean Teacher’s Ability to Manage Non-face-to-face Classes A Study on Job Stress and Job Challenge
Authors:   The Korean Society of Culture and Convergence, Jo Ahra , Kim Hyeeun
Published: June 2022

The purpose of this study is to empirically identify the job stress and job challenge perceived by Korean teachers in the non-face-to-face classroom environment caused by COVID-19. Today’s evolving technological advances and the variable COVID-19 have had a direct impact on the change in the educational field. The introduction of the non-face-to-face class method changed the educational paradigm and had an effect on the job of the professor. In particular, in the case of Korean classes for foreigners, there was a lot of trouble from designing the class method. In order to achieve the purpose of this study, qualitative data were collected and analyzed from 5 Korean language teachers at metropolitan universities. The research method was a semistructured interview based on a questionnaire, and took three forms: face-to-face, telephone, and video. The results of interview data analysis were able to draw contradictory results in job stress and job challenge. Job stress increased due to non-face-to-face classes, but on the other hand, job challenge also increased. The factors contributing to job stress were the application of the online job environment, the difficulty of interaction between teachers and learners, and the phenomenon of work overload. Nevertheless, the professor had the characteristic of being able to demonstrate job challenge by producing high-quality educational contents. Factors affecting job challenge include competency development and expansion, creative idea creation, and job prospect search. Based on the analysis results, conclusions and implications for improving job stress and job challenge of Korean teachers were presented.
Artists’ Mental Health Research: Trend and Future Directions
Authors:   The Korean Society of Culture and Convergence, Jang Yeonjoo , Choi Nayeun
Published: June 2022

This research aims to define artists by examining relevant laws and previous research related to the mental health of Korean artists. Further, this study aims to present the direction for future research on mental health of artists by systematically analyzing research trends. The research questions to be examined in this study are, first, what is the current status of research related to artists' mental health? Second, what research topics are related to artists' mental health? Third, what is the future of research on artist's mental health? A total of 26 articles and dissertations published in Korean journals from 1998 to 2021 were analyzed in this study. By year, research field, research method, presence of term definition, academic field of the researcher, and subject trend were used as criteria for analysis. As a result, research on artist’s mental health has increased since 2014. However, there is a lack of research on various aspects of arts by not having a clear definition of the artist. Furthermore, the rate of research done by non-mental health related fields was higher than mental health-related fields. The research on artists’ mental health such as depression, suicide, and anxiety were the most frequently studied. As future research directions, ‘defining of terms in the art field’, ‘activating interdisciplinary research’, ‘needing for qualitative data’, ‘studying the occupational gap and creative hardships of artists’, as well as ‘expanding on positive variables in research affecting the quality of life of artists’ were proposed. Finally, the limitations and significance of this study were discussed.
Expansion of Community-Based Art Therapy by Exploring Intersections in Public Art and Therapeutic Implications
Authors:   The Korean Society of Culture and Convergence, Lee Hyunjung , Park Yunjuo , Park Sojung
Published: June 2022

The purpose of this study is to explore intersections between art therapy and public art and to reveal therapeutic implications in order to expand community-based art therapy to establish psychoemotional safety network for the community. This study attempted to analyze and examine the cases of community-based art therapy and community-based public art with similar categorical characteristics, focusing on overseas cases. As a result of the study, the similarities and differences between the two fields were revealed in goals, awareness of social issues, use of communities, roles of art, and therapeutic properties. Based on this, this study proposed a point that can be introduced in community-based art therapy by referring to the precedent of public art, and expect collaboration in the two fields based on the safety of art therapy and the dynamics of public art. It is hoped that this study will be used as basic data for the establishment of the concept of community-based art therapy and specific methodology that is insufficient in Korea.
A Comparative Study on Writing Style of Chinese Korean Learners for Academic purposes in Korean and Writing Style in Chinese
Authors:   The Korean Society of Culture and Convergence, Cho Eunyoung , Cong Ni
Published: June 2022

The purpose of this study was to compare the Korean writing style and Chinese writing style on the same subject, with a focus on Chinese Korean learners for academic purposes. A total of 28 Chinese international students performed the writing task on the same subject. As a result, the difference in syntactic proficiency was not significant between the Korean essays and the Chinese. However, it confirmed that the pattern of errors in Korean writing was influenced from the native language. In addition, the writer's stance and voice expression patterns can often enhance the genre characteristics of the essay, and it was found that more expressions revealing the writer's stance and voice appeared in the Chinese essays. In addition, the expressions in the Korean essays and the expressions in the Chinese essays were often different. As writing activities using digital devices increase, it is expected that sentences and awkward expressions of translation will increase. Therefore, applying the understanding of error patterns based on the characteristics of the native language to the teaching field will greatly help Korean learners write for academic purposes.
Utilization of Oral Records and Urban Regeneration Projects in Gunsan: From the Perspective of Village History Reconstruction into Cultural Contents
Authors:   The Korean Society of Culture and Convergence, Cho Sungsil , Lee Jungwoo
Published: June 2022

The purpose of this study is to reconstruct the history of the village based on the oral records of villagers carried out as part of the cultural urban regeneration project in Gunsan City. In addition, it intends to review the use of the oral records into content. The study area is Sinheung-dong located in the old downtown of Gunsan. This neighborhood is one of Gunsan’s representative tourist destinations, in which there are various modern cultural heritages that represent the modern era. The historical background of tourist destinations centered on Sinheung-dong is the Japanese colonial period, in particular the 1930s. This study raises questions about the fact that the identity of Sinheung-dong is limited to one period of Japanese colonial rule and becomes a tourism resource. Therefore, this study tried to explore the various modern experiences of the local residents of Gunsan based on the oral records of residents currently living in Sinheung-dong, and to reconstruct the history and local identity of the village based on them. Finally, this paper explored ways the collected oral records could function as exhibition contents for major cultural facilities in Sinheung-dong village, which were created as a result of the urban regeneration project.
1950s The World of Health and Tuberculosis-enlightenment of Masan
Authors:   The Korean Society of Culture and Convergence, Han Jeongho
Published: June 2022

The study examined the TB enlightenment phenomenon and the meaning and characteristics of he World of Health in Masan in the early 1950s, targeting The World of Health(1-8) published at the National Masan Tuberculosis Care Center. In this study, as the primary work, The World of Health’s bibliography, media tendencies, editorial direction and media characteristics were introduced in the dimension of media introduction. First, the background of the publication of the Korean Tuberculosis Association bronchial The World of Health started from the magazine You, which was published at the National Masan Tuberculosis Nursing Home in the early 1950s. Kim Daekyu, who played a pivotal role in the work, followed the first issue of TB enlightenment The World of Health on July 1, 1953, followed by the second(1953. 8) and third(1953. 9), 4(1953. 12), 5(1954. 1), 6(1954. 3), 7(1954. 4), and 8(1954. 5) were published in Masan. Since then, The World of Health has been transferred to the Tuberculosis Association without any conditions in December 1954. Next, looking at the bibliography and editorial characteristics of The World of Health(1-8), This is the oriented ideology of the magazine media, science, humanism and warm affection, and kept the system unchanged until the eighth even in difficult circumstances. And this included doctors’ columns on tuberculosis, patient's battling period, literary poetry and prose, introduction of medical facilities and current affairs. In particular, the fact that the emphasis on each book is on the comments of specialists has given meaning as a tuberculosis enlightenment magazine. At that time, The World of Health was used as a forum for information exchange among patients along with the delivery of knowledge about diseases, and played a role as a cultural magazine leading the discourse of the time on tuberculosis. In the future, discussions on The World of Health overall should be further deepened. For example, the study on the status and meaning of the cultural center for tuberculosis prevention in the bibliographical dimension, the characteristics of tuberculosis literature or medical literature in the literary dimension, and the role and prospect of tuberculosis enlightenment in the social dimension should follow. In conclusion, The World of Health has its value and status as a tuberculosis enlightenment magazine through medical knowledge and discourse on tuberculosis treatment and treatment.In this article, the meaning and characteristics of Masan’s TB discourse, which had a reputation as a city of recreation and healing, and media The World of Health(1-8), which played a pivotal role in TB enlightenment, were introduced in the dimension of local research. However, in order to understand the TB history in Korea, it is necessary to encourage Masan’s multifaceted and in-depth approach to TB enlightenment and TB literature.
A Trend in Research on the Psychological Healing Effects of Travel
Authors:   The Korean Society of Culture and Convergence, Du Kyunghee , Lee Eunjoo , Lee Yoonjung
Published: June 2022

This study examines the psychological healing effects of travel since 2010. Healing tourism remains popular, as the healing craze has spread throughout society. In COVID-19, where travel is not free, the importance of positive traveler psychology may increase. 52 papers published since 2010 were evaluated using publication years, academic journal titles, independent variables, dependent variables, and research methods in order to assess research trends. As a result of the study, healing mania began in 2011, but research accelerated in 2014, with the greatest number of papers published between 2016 and 2020, when COVID-19 commenced. Tourism and leisure periodicals, as well as environmental and rural periodicals, discussed the therapeutic effects of travel. 78.8 percent of studies on the healing effects of travel employed quantitative research, including t-test analysis. The primary instruments for measuring healing effects were life satisfaction, positive/negative emotion, stress, and depression, while the independent variables included travel experience, travel destination, and interaction between destination and experience. In addition to quantitative research, this study concluded that research on travel's psychological healing effect should be conducted, and the source of the measurement instrument should be described. To represent the healing effect of travel, it appears necessary to treat the dependent variable as a differentiated variable when establishing its value. This study could lead to additional research on the psychological benefits of travel.
Kant and Burke’s Sublime in Werner Herzog’s Films: The Quest for an Ecstatic Truth
Authors: Castello Branco Patrícia
Published: June 2022

The German filmmaker Werner Herzog controversially associates “truth” and “reality” in film with Kant’s notion of the sublime by explicitly treating the sublime as a key element in developing his notion of ecstatic truth. I critically examine Herzog’s interpretation of Kant’s sublime and the relations he establishes between the sublime and his own key aesthetic notion of ecstatic truth. I examine how the sublime in Herzog’s films arises from encounters with the overwhelming force and power of nature experienced by his characters in the feature films and participants in the documentaries. I question whether Kant’s conception of the sublime is the one that best aligns with Herzog’s aesthetic aims and I contrast Kant’s transcendental sublime with what I describe as the physiological sublime of Edmund Burke. I draw some conclusions about the moral dimension of the sublime in the context of the relationship between humans and nature within the frame of contemporary ecological concerns.
The Disrobing of Aphrodite: Brigitte Bardot inLe Mépris
Authors: Keohane Oisín
Published: June 2022

This article examines a number of philosophical concepts that are at stake in the visual culture of the nude. It particularly focuses on Aphrodite’s appearance, or rather, what I call her exposed concealment, in Jean-Luc Godard’s 1963 Le Mépris. A film, I argue, which is not only concerned with Aphrodite and the figure of the female nude via Brigitte Bardot, but which also explores the very idea of the sex goddess in cinema. In the first section I introduce arguments from T.J. Clark about the changing status of the nude in nineteenth-century France. In the second section, having introduced Kenneth Clark’s work on Aphrodite, I outline Michael Williams’ work on the archaeology of divine stardom and discuss my disagreement with the way Ginette Vincendeau and Colin Gardner interpret Bardot in the film. In the longest section, the third, I examine several shots in Le Mépris in conversation with Stanley Cavell and argue that the nude scenes both invoke and rework the pictorial language of nudity found in the history of painting and sculpture, as well as Bardot’s film back catalogue, and I conclude by suggesting the film provides an indirect critique of what Hegel says about female nudity.
Henrician Homer: English Verse Translations from the Iliad and Odyssey, 1531–1545
Authors: Colley John
Published: June 2022

Verse translations from the Iliad and Odyssey embedded in Thomas Elyot’s Gouernour, Roger Ascham’s Toxophilus, and Nicholas Udall’s Apophthegmes might seem the poor cousins of longer and better-known Homer translations by poets such as George Chapman. But this article, which pays close literary-critical attention to Elyot’s, Ascham’s, and Udall’s Homer translations, argues that they play an important and mostly untold part in a larger story concerning the translation of Homer into English, not to mention the vernacular translation of ancient Greek literature in England in the sixteenth century. These fragmentary translations reveal that early Tudor writers had a wider array of options in their methods of classical translation than has hitherto been appreciated. They also call for more nuanced consideration of the diverse intellectual, political, and literary contexts that spurred poetic innovation in late Henrician England.
Testing for price transmission in Thailand’s oil palm and palm oil markets: an empirical study using time series analysis
Authors: Charoenrit Pacharapon P. , Jatuporn Chalermpon , Pantavisid Sombat , Suvanvihok Vasu , Rueangrit Paisan
Published: June 2022

This study aims to analyze the price transmission within the supply chains of Thailand’s oil palm and palm oil markets. The study employs time-series data from January 2012 to December 2019, which comprises 96 monthly price samples, using econometric analysis, namely, the ADF unit root, Granger causality, co-integration, and error correction model. The empirical results show that (1) there are four causal relationships from crude palm oil price running to palm fruit price, the wholesale price of bottle-refined palm oil, and the retail price of bottle-refined palm oil, and from the wholesale price of bottle refined palm oil running to the wholesale price of gallon refined palm oil. The results further reveal that (2) the palm fruit price has the highest price transmission efficiency, followed by the wholesale price of bottle-refined palm oil, the retail price of bottle-refined palm oil and the wholesale price of gallon refined palm oil. The findings conclude that the crude palm oil price influences the pricing in Thailand’s oil palm and palm oil markets.
Application of socially-oriented and legal innovative practices in the urban transport flow logistics and tourism
Authors: Sushchenko Roman , Zapara Yaroslav , Saienko Volodymyr , Kostiushko Viktor , Lytvynenko Larysa , Pron Svitlana
Published: June 2022

The relevance of the study is determined by the public interest in logistic support of urban transport flows and tourism. The aim of the article is to determine the best innovative practices of providing transport and tourist flows by different urban transport modes, and also to identify prospects for their use in conditions of domestic markets. The study found that according to the German experience as one of the best practices, the priority of the development should be given to the rail transport as a key component of urban transport and logistics infrastructure, including the tourism sector. It was determined that the integrated urban intelligent transport system includes the traffic management system, the public transport management system, the parking management system and the logistics flow management system, considering the impact of tourism. The regularities of the influence of factors on the dynamics of urban transport flows were determined based on a critical analysis of a number of specific cases. The prospects for the development of rural areas adjacent to such cities were determined, given the development of transport and logistics infrastructure of the country. The materials of the study can be useful for employees of the transport and logistics sector and the sphere of tourist services, researchers, graduate students, doctoral students, and government officials.
Logistics approaches to stabilization and development of tourism activities during the pandemic
Authors: Aloshynskyi Yevhen , Remzina Natalia , Tregubov Oleksandr , Shevchenko Valentyna , Britchenko Igor
Published: June 2022

The relevance of the problem under study is stipulated by the need to stabilize the market for tourist services in existing restrictions caused by the SARS-CoV-2 pandemic. The purpose of the research: The purpose of the article is to develop integrated measures for the formation of transport and logistics clusters to increase the synergetic effect of the proposed activities aimed at raising the mobility level of potential consumers of tourist services. Methods of the research: The main research methods include predicting and simulating; they make it possible to consider the problem of forming the package of tourist and transport services as a process of targeted monitoring and optimization of tourist routes. Results of the research: The article presents the concept of the creation of the network of transport and tourist clusters in Poland and its functioning; large rail carriers are the core of the clusters. Practical significance: The proposed models for the formation of a package of tourist services are aimed at increasing the synergetic effect for all cluster participants, as well as at the development of tourist activities during the pandemic.
Evaluation of differnt sowing methods for enhancing productivity and water use efficiency of wheat under limited water condition
Authors: Riaz Atif , Aziz Mudassir , Ghaffar Abdul , Ahmed Wazir , Mubeen Khuram , Usman Muhammad
Published: June 2022

Drought is a serious threat for food security worldwide. Wheat is most popular staple crop of world severely affected by drought at water sensitive crop growth stages. Selection of appropriate sowing method is very important for improving water use efficiency. A field trial was conducted in winter season of 2018-19 to investigate the impact of different sowing methods on water use efficiency of spring wheat exposed to water deficit at anthesis. Wheat was sown with three different sowing methods viz. broadcast sowing, drill sowing and augmented furrow sowing and two different irrigation regimes i.e. normal irrigation and water deficit at anthesis stage. All agronomic practices were kept uniform throughout the experiment. Different sowing methods and irrigation regimes significantly affected grain and straw yield of wheat. Among different sowing methods, wheat crop sown with augmented furrow method produced maximum plant height (111 cm), no. of tillers per m2 (427),  1000 grain weight (37.93 g), grain yield (4.73 t ha-1) and straw yield (4.24 t ha-1). Maximum irrigation water use efficiency (17.72 kg ha-1 mm-1) and benefit cost ratio (1.34) was recorded in augmented furrow method of sowing of wheat exposed to water deficit at anthesis stage.
Improving Business Processes by Applying the Kaizen Philosophy in a Macedonian Textile Company
Authors: Mitreva Elizabeta , Janeva Aneta
Published: June 2022

This paper presents the research aimed at developing a solution for advancing of business processes in a Macedonian textile company, by implementing techniques and methods of the Kaizen philosophy. The main objective of the paper is to make a comprehensive analysis of the factors that enable the improvement of the overall production process, thus achieving greater effectiveness and efficiency in the production operations, as well as greater market competitiveness. The solutions were based on the concept of improving the efficiency of operations, especially in the area of organization of production operations, in a way that enabled complete elimination of errors, greater productivity and increased quality of the final product, within a precisely defined time frame. Additionally, the paper also shows the key elements from the implementation of the Kaizen philosophy in the process of competitiveness improvement of the company.
THE EFFECT OF SEMENDO COFFEE ON THE NUMBER OF FIBROBLAST CELLS IN GINGIVA WOUND OF RATS
Authors: Sulistiawati Sulistiawati , Awalia Hema , Yasmin Ulfa , Dwi Rosada Sintya , Namira Alya
Published: June 2022

Introduction: Gingiva is a keratinized epithelium tissue that surrounds the tooth and protects the tissue beneath it, which is often wounded. Sumatran robusta coffee, semendo coffee, consists of active compounds such as polyphenol, alkaloid, and saponin that can increase fibroblast amount during the wound healing process. This study aims to determine the effect of semendo coffee (Coffea canephora) extract on fibroblast amount on gingiva wound of Rattus norvegicus. Methods: The true experimental study with a post-test-only control group design was confirmed in this study. This study used twenty-four rats (Rattus norvegicus) divided into 4 groups. The gingival injury was performed on mandible gingiva using a punch biopsy method with a 2mm diameter. The gel was given according to the treatment group 2 times a day for 7 days. Semendo coffee extract at 5%, 20%, and 40%, was applied to the wound treatment groups, while placebo gel was applied to the control group. Euthanasia was delivered on the 8th day, then histological preparation was made. The amount of fibroblast was analyzed by Olympus software. Result: The results showed that the Semendo coffee extract could significantly improve the number of fibroblast cells compared to the control groups. The highest fibroblast amount was found in the group with Semendo coffee extract at a concentration of 40%. Conclusion: Semendo coffee extract at 5%, 20%, and 40% increased the amount of fibroblast on the gingival wound in Rattus norvegicus.
Integrins Cooperate With the EGFR/Ras Pathway to Preserve Epithelia Survival and Architecture in Development and Oncogenesis
Authors: Valencia-Expósito Andrea , Gómez-Lamarca M. Jesús , Widmann Thomas J. , Martín-Bermudo María D.
Published: June 2022

Adhesion to the extracellular matrix (ECM) is required for normal epithelial cell survival. Disruption of this interaction leads to a specific type of apoptosis known as anoikis. Yet, there are physiological and pathological situations in which cells not connected to the ECM are protected from anoikis, such as during cell migration or metastasis. The main receptors transmitting signals from the ECM are members of the integrin family. However, although integrin-mediated cell-ECM anchorage has been long recognized as crucial for epithelial cell survival, the in vivo significance of this interaction remains to be weighed. In this work, we have used the Drosophila wing imaginal disc epithelium to analyze the importance of integrins as survival factors during epithelia morphogenesis. We show that reducing integrin expression in the wing disc induces caspase-dependent cell death and basal extrusion of the dead cells. In this case, anoikis is mediated by the activation of the JNK pathway, which in turn triggers expression of the proapoptotic protein Hid. In addition, our results strongly suggest that, during wing disc morphogenesis, the EGFR pathway protects cells undergoing cell shape changes upon ECM detachment from anoikis. Furthermore, we show that oncogenic activation of the EGFR/Ras pathway in integrin mutant cells rescues them from apoptosis while promoting their extrusion from the epithelium. Altogether, our results support the idea that integrins promote cell survival during normal tissue morphogenesis and prevent the extrusion of transformed cells.
Diabetes and SARS-CoV-2–Is There a Mutual Connection?
Authors: Jedrzejak Anna P. , Urbaniak Edyta K. , Wasko Jadwiga A. , Ziojla Natalia , Borowiak Malgorzata
Published: June 2022

SARS-CoV-2, a newly emerged virus described for the first time in late 2019, affects multiple organs in humans, including the pancreas. Here, we present the bilateral link between the pathophysiology of diabetes and COVID-19, with diabetes being COVID-19 comorbidity, and a complication of SARS-CoV-2 infection. Analysis of clinical data indicates that patients with chronic conditions like diabetes are at increased risk of severe COVID-19, hospitalization, ICU admission, and death compared to the healthy subjects. Further, we show that SARS-CoV-2 infection might be also associated with the development of new-onset diabetes and diabetic ketoacidosis. We then discuss the options for studying SARS-CoV-2 infection in pancreatic settings, including the use of human pluripotent stem cell-derived pancreatic organoids. Further, we review the presence of SARS-CoV-2 receptors in different pancreatic cell types and the infection efficiency based on pancreatic sections from COVID-19 patients and primary human islet in vitro studies. Finally, we discuss the impact of SARS-CoV-2 infection on human pancreatic cell homeostasis, focusing on β-cells.
Tandem Mass Tagging Based Identification of Proteome Signatures for Reductive Stress Cardiomyopathy
Authors: Sunny Sini , Jyothidasan Arun , David Cynthia L. , Parsawar Krishna , Veerappan Arul , Jones Dean P. , Pogwizd Steven , Rajasekaran Namakkal S.
Published: June 2022

Nuclear factor erythroid 2-related factor 2 (NRF2), a redox sensor, is vital for cellular redox homeostasis. We reported that transgenic mice expressing constitutively active Nrf2 (CaNrf2-TG) exhibit reductive stress (RS). In this study, we identified novel protein signature for RS-induced cardiomyopathy using Tandem Mass Tag (TMT) proteomic analysis in heart tissues of TG (CaNrf2-TG) mice at 6–7 months of age. A total of 1,105 proteins were extracted from 22,544 spectra. About 560 proteins were differentially expressed in TG vs. NTg hearts, indicating a global impact of RS on the myocardial proteome. Over 32 proteins were significantly altered in response to RS -20 were upregulated and 12 were downregulated in the hearts of TG vs. NTg mice, suggesting that these proteins could be putative signatures of RS. Scaffold analysis revealed a clear distinction between TG vs. NTg hearts. The majority of the differentially expressed proteins (DEPs) that were significantly altered in RS mice were found to be involved in stress related pathways such as antioxidants, NADPH, protein quality control, etc. Interestingly, proteins that were involved in mitochondrial respiration, lipophagy and cardiac rhythm were dramatically decreased in TG hearts. Of note, we identified the glutathione family of proteins as the significantly changed subset of the proteome in TG heart. Surprisingly, our comparative analysis of NGS based transcriptome and TMT-proteome indicated that ~50% of the altered proteins in TG myocardium was found to be negatively correlated with their transcript levels. In association with the altered proteome the TG mice displayed pathological cardiac remodeling.
Atrial Mitral and Tricuspid Regurgitation: Sex Matters. A Call for Action to Unravel the Differences Between Women and Men
Authors: Gual-Capllonch Francisco , Sáenz de Ibarra José Ignacio , Bayés-Genís Antoni , Delgado Victoria
Published: June 2022

Atrial functional regurgitation is caused by atrioventricular annulus dilation, with normal leaflets and ventricular dimensions and function within the normal range. Its occurrence, in both mitral and tricuspid valves, implies a worse prognosis due to the hemodynamic derangement they produce, but also constitutes a marker of greater comorbidity and more advanced disease. Predisposing conditions for these heart valve dysfunctions are mainly atrial fibrillation and heart failure with preserved ejection fraction. However, other factors like female sex also may be involved and influence their incidence, especially for atrial tricuspid regurgitation. In the present review, we analyze sex differences in the reported prevalence of atrial mitral and tricuspid regurgitation, and suggest possible mechanisms involved. Finally, we underline potential therapeutic and preventive strategies to reduce the burden of these heart valve disorders and discuss research gaps.
Plasma Homocysteine Level Is Independently Associated With Conventional Atherogenic Lipid Profile and Remnant Cholesterol in Adults
Authors: Zhou Liyuan , Liu Jia , An Yu , Wang Ying , Wang Guang
Published: June 2022

Background

Homocysteine (Hcy) is an independent risk factor for cardiovascular disease, while mechanisms are unclear. Despite inconsistent and limited, epidemiological and experimental studies indicated that hyperhomocysteinemia (HHcy) affected lipid metabolism. This study aims to investigate the association of plasma Hcy with traditional lipid profiles and remnant cholesterol (RC) in Chinese adults.

Methods

In total, 7,898 subjects aged 20–79 years who underwent a physical examination at Beijing Chao-Yang Hospital in Beijing were included in this study. Fasting plasma total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), lipoprotein (a) [Lp(a)], Hcy, and other metabolic risk factors were measured by routine automated laboratory methods. RC was calculated as TC minus HDL-C and LDL-C. The linear regression model and logistic regression model were used to assess the relationship between Hcy and lipids after adjusting potential confounders.

Results

Of the subjects, the median level of plasma Hcy was 13.0 μmol/L and 32.3% had HHcy. Plasma Hcy was negatively associated with HDL-C, ApoA1, and Lp(a) and positively associated with TG levels after adjusting age, sex, body mass index, blood pressure, alanine transaminase, aspartate transaminase, creatinine, uric acid, and glucose. HHcy significantly increased the risk of low HDL-C [odds ratio (OR) 1.26; 95%CI (1.11–1.44); p < 0.001]. The net mediation effects of ApoA1 on the relationship between Hcy and HDL-C before and after adjusting confounders were 46.9 and 30.6%, respectively. More interestingly, the RC level was significantly elevated in subjects with HHcy after adjusting other influencing factors (p = 0.025). Hcy presented a positive correlation with RC levels after adjusting the above confounding factors (β = 0.073, p = 0.004), and the correlation was still significant even after controlling other lipids, including TG, LDL-C, HDL-C, ApoA1, ApoB, and Lp(a).

Conclusion

Our study showed that plasma Hcy was not only significantly associated with conventional atherogenic lipids but also independently correlated with RC levels beyond other lipids after controlling potential confounders. This finding proposes that identifying Hcy-related dyslipidemia risk, both traditional lipids and RC residual risk, is clinically relevant as we usher in a new era of targeting Hcy-lowering therapies to fight against dyslipidemia or even cardiovascular disease.
A 3-Year Single Center Experience With Left Atrial Pressure Remote Monitoring: The Long and Winding Road
Authors: Restivo Attilio , D'Amario Domenico , Paglianiti Donato Antonio , Laborante Renzo , Princi Giuseppe , Cappannoli Luigi , Iaconelli Antonio , Galli Mattia , Aspromonte Nadia , Locorotondo Gabriella , Burzotta Francesco , Trani Carlo , Crea Filippo
Published: June 2022

Background

Despite continuous advancement in the field, heart failure (HF) remains the leading cause of hospitalization among the elderly and the overall first cause of hospital readmission in developed countries. Implantable hemodynamic monitoring is being tested to anticipate the clinical exacerbation onset, potentially preventing an emergent acute decompensation. To date, only pulmonary artery pressure (PAP) sensor received the approval to be implanted in symptomatic heart failure patients with reduced ejection fraction. However, PAP's indirect estimation of left ventricular filling pressure can be inaccurate in some contexts.

Methods

The VECTOR-HF study (NCT03775161) is examining the safety, usability and performance of the V-LAP system, a latest-generation device capable of continuously monitoring left atrial pressure (LAP). In our center, five advanced HF patients have been enrolled. After confirmation of the transmitted data reliability, LAP trends and waveforms have guided therapy optimization. The aim of this work is to share clinical insights from our center preliminary experience with V-LAP application.

Results

Over a median follow-up time of 18 months, LAP–based therapy optimization managed to reduce intracardiac pressure over time and no hospital readmission occurred. This result was paralleled by an improvement in both functional capacity (6MWT distance 352.5 ± 86.2 meters at baseline to 441.2 ± 125.2 meters at last follow-up) and quality of life indicators (KCCQ overall score 63.82 ± 16.36 vs. 81.92 ± 9.63; clinical score 68.47 ± 19.48 vs. 83.70 ± 15.58).

Conclusion

Preliminary evidence from V-LAP application at our institution support a promising efficacy. However, further study is needed to confirm the technical reliability of the device and to exploit the clinical benefit of left-sided hemodynamic remote monitoring.
Ross Procedure in the era of Handmade-Valved Conduits for Right Ventricular Outflow Tract Reconstruction in Children: Short-Term Surgical Outcomes
Authors: Dong Wei , Chen Dian , Jiang Qi , Hu Renjie , Qiu Lisheng , Zhu Hongbin , Zhang Wen , Zhang Haibo
Published: June 2022

Objective

Ross procedure is considered as the “gold standard” for aortic valve replacement, but the conduits used for right ventricular outflow tract (RVOT) reconstruction, such as homografts and bovine jugular vein (BJV) conduits, are of limited availability in China. Handmade expanded polytetrafluoroethylene-valved conduits (HVCs) have been used recently as the alternative for RVOT reconstruction, but their specific experience in Ross procedure is limited in the literature.

Methods

This was a retrospective review of 27 children who underwent Ross procedure in our center from January 2018 to January 2022.

Results

Mean age at surgery was 8.0 ± 3.8 years. During the study period, BJV conduits were used for RVOT reconstruction in 6 patients (22%), and HVCs were used in 21 patients (78%). Median conduit size was 20 mm (range, 16–24 mm), and mean conduit Z-score was +0.8 ± 0.9. Median time for cardiopulmonary bypass was 158 min (range, 109–275 min), and mean time for aortic crossclamping was 110 ± 21 min. There was no early mortality. During a median follow-up time of 1.4 years (range, 0.1–3.7 years), 3 patients (11%) with BJV conduits had peak conduit velocity of > 3.5 m/s; 3 patients (11%) with HVCs developed moderate conduit insufficiency; no patients had more than moderate conduit insufficiency. Three patients with BJV conduits had 5 reinterventions, and all received conduit replacement with HVCs.

Conclusion

HVC is an appealing alternative to BJV conduit for RVOT construction for children undergoing Ross procedure, with favorable short-term outcomes.
Waveform Energy Focusing Tomography With Passive Seismic Sources
Authors: Hu Yueqiao , Li Junlun , Zhang Haijiang
Published: June 2022

By taking advantage of the information carried by the entire seismic wavefield, Full Waveform Inversion (FWI) is able to yield higher resolution subsurface velocity models than seismic traveltime tomography. However, FWI heavily relies on the knowledge of source information and good initial models, and could be easily trapped into local minima caused by cycle skipping issue because of its high nonlinearity. To mitigate these issues in FWI, we propose a novel method called Waveform Energy Focusing Tomography (WEFT) for passive seismic sources. Unlike conventional FWI, WEFT back-propagates the seismic records directly instead of the data residuals, and updates the velocity models by maximizing the stacking energy for all the moment tensor components from back-propagated wavefields around the sources. Therefore, except for source locations and origin times, WEFT does not require other source attributes in advance for the inversion. Since WEFT does not aim at fitting synthetic and observed waveforms, it has lower nonlinearity and is less prone to the cycle skipping issue compared to FWI. For the proof of concept, we have validated WEFT using several 2D synthetic tests to show it is less affected by inaccurate source locations and data noise. These advantages render WEFT more applicable for tomography using passive seismic sources when the source information is generally not accurately known. Although the inverted model from WEFT is inevitably influenced by the source distribution as well as its radiation patterns, and its resolution is likely lower than that of FWI, it can act as an intermediate step between traveltime tomography and FWI by providing a more reliable and accurate velocity model for the latter.