bluuebunny/crossref_metadata_2025 huggingface dataset:
Genomic study of Acinetobacter baumannii strains co-harboring blaOXA-58 and blaNDM-1 reveals a large multidrug-resistant plasmid encoding these carbapenemases in Brazil
Authors: Rodrigues Daiana Cristina Silva , Silveira Melise Chaves , Pribul Bruno Rocha , Karam Bruna Ribeiro Sued , Picão Renata Cristina , Kraychete Gabriela Bergiante , Pereira Felicidade Mota , de Lima Rildo Mendes , de Souza Antonio Kleber Gomes , Leão Robson Souza , Marques Elizabeth Andrade , Rocha-de-Souza Cláudio Marcos , Carvalho-Assef Ana Paula D'Alincourt
Published: July 2024

Introduction

Acinetobacter baumannii contributes significantly to the global issue of multidrug-resistant (MDR) nosocomial infections. Often, these strains demonstrate resistance to carbapenems (MDR-CRAB), the first-line treatment for infections instigated by MDR A. baumannii. Our study focused on the antimicrobial susceptibility and genomic sequences related to plasmids from 12 clinical isolates of A. baumannii that carry both the blaOXA-58 and blaNDM-1 carbapenemase genes.

Methods

Whole-genome sequencing with long-read technology was employed for the characterization of an A. baumannii plasmid that harbors the blaOXA-58 and blaNDM-1 genes. The location of the blaOXA-58 and blaNDM-1 genes was confirmed through Southern blot hybridization assays. Antimicrobial susceptibility tests were conducted, and molecular characterization was performed using PCR and PFGE.

Results

Multilocus Sequence Typing analysis revealed considerable genetic diversity among blaOXA-58 and blaNDM-1 positive strains in Brazil. It was confirmed that these genes were located on a plasmid larger than 300 kb in isolates from the same hospital, which also carry other antimicrobial resistance genes. Different genetic contexts were observed for the co-occurrence of these carbapenemase-encoding genes in Brazilian strains.

Discussion

The propagation of blaOXA-58 and blaNDM-1 genes on the same plasmid, which also carries other resistance determinants, could potentially lead to the emergence of bacterial strains resistant to multiple classes of antimicrobials. Therefore, the characterization of these strains is of paramount importance for monitoring resistance evolution, curbing their rapid global dissemination, averting outbreaks, and optimizing therapy.
Duchenne muscular dystrophy in Saudi Arabia: a review of the current literature
Authors: Aldharee Hitham
Published: July 2024

In the past three decades, significant improvements have occurred in the study of Duchenne muscular dystrophy (DMD). DMD is a rare, severe neuromuscular disease that causes death due to cardiovascular and respiratory complications among affected boys. Since the 1980s, ongoing preclinical and clinical studies have been conducted to explore the disease in depth and discover potential therapeutic strategies. In Saudi Arabia, it is unclear whether health services and research efforts are keeping pace with global achievements. Therefore, this review aims to explore the diagnostic and management strategies and research efforts in Saudi Arabia over the past three decades. I searched the PubMed/Medline, Scopus, and Web of Science databases and included all published articles on the epidemiology, genetics, diagnosis, and management of DMD/BMD in this review. The findings suggest a lack of local standardized diagnostic strategies, a poor understanding of epidemiology and common pathogenic variants, and a critical need for preclinical and clinical research. At the time of writing, no such comprehensive review has been published. Challenges, limitations, and future perspectives are also discussed in this article.
Co-occurrence of tethered cord syndrome and cervical spine instability in hypermobile Ehlers-Danlos syndrome
Authors: Gensemer Cortney , Daylor Victoria , Nix Jared , Norris Russell A. , Patel Sunil
Published: July 2024

The Ehlers-Danlos Syndromes (EDS) represent a group of hereditary connective tissue disorders, with the hypermobile subtype (hEDS) being the most prevalent. hEDS manifests with a diverse array of clinical symptoms and associated comorbidities spanning the musculoskeletal, neurological, gastrointestinal, cardiovascular, and immunological systems. hEDS patients may experience spinal neurological complications, including cervico-medullary symptoms arising from cranio-cervical and/or cervical instability/hypermobility, as well as tethered cord syndrome (TCS). TCS is often radiographically occult in nature, not always detectable on standard imaging and presents with lower back pain, balance issues, weakness in the lower extremities, sensory loss, and bowel or bladder dysfunction. Cervical instability due to ligament laxity can lead to headaches, vertigo, tinnitus, vision changes, syncope, radiculopathy, pain, and dysphagia. TCS and cervical instability not only share clinical features but can also co-occur in hEDS patients, posing challenges in diagnostics and clinical management. We present a review of the literature and a case study of a 20-year-old female with hEDS, who underwent surgical interventions for these conditions, highlighting the challenges in diagnosing and managing these complexities and underscoring the importance of tailored treatment strategies to improve patient outcomes.
Case report: A case with atypical presentation oftesticular choriocarcinoma
Authors: Zhong Bin , Zhang Tao , Dong Yi , Yin Wei , Zhang Jian-Xin , Jin Wei-Dong
Published: July 2024

Testicular choriocarcinoma is a relatively rare malignancy with a highly aggressive nature. Timely diagnosis and treatment can help prolong the survival of patients and even cure them. This case reports a 29-year-old male who presented to the clinic for a month with epigastric pain. On examination, a massive mass of approximately 9*10 cm could be palpated in the upper abdomen. When asked about his previous history, the patient only described a history of a right inguinal hernia that had been repaired 12 years earlier. The admission diagnosis was considered the retroperitoneal tumor, which was found to have metastasized to the liver and lungs after the completion of relevant tests. We then performed a CT-guided lune puncture biopsy on day 8 of admission. The biopsy pathology suggested metastatic cancer was considered. As the symptoms of tumor compression gradually worsened, we performed surgical treatment (retroperitoneal tumor resection + partial duodenal resection + enteroanastomosis) on day 13 of admission. The postoperative pathology was choriocarcinoma. We subsequently conducted a detailed inquiry with the patient’s family about his medical history and found a history of inguinal testicle. Through testicular ultrasound examination, it was preliminarily determined to be testicular choriocarcinoma (not yet pathologically confirmed). We wanted to start salvage chemotherapy as soon as possible after surgery. However, the patient’s postoperative condition was poor, with rapid progression of hepatopulmonary metastases and gradually increased thyrotoxicosis, and we started salvage chemotherapy (EP regimen: etoposide and cisplatin) on postoperative day 12. However, the patient was forced to stop due to a severe chemotherapy reaction and died of respiratory and cardiac arrest in the hospital. For male patients with retroperitoneal mass, the possibility of germ-cell neoplasm should first be excluded. By inquiring in detail about a history of cryptorchidism and in the initial days of hospitalization, testicular exploration, ultrasounds, and serum tumor markers (AFP, β-HCG) tests can be conducted to rule out the possibility of germ-cell neoplasm, thereby preventing misdiagnosis and treatment delays. If the clinical diagnosis is metastatic germ-cell tumor with severe symptoms of metastatic disease, surgery should never be used as the initial treatment.
Associations between oxygen saturation Index and oxygenation index in neonates with congenital diaphragmatic hernia
Authors: Ali Kamal , Algarni Saleh S. , Alotaibi Abdullah M. , Aljuaid Nemer , Ghazwani Abadi , Alshreedah Saad , Alotaibi Naif , Alanazi Ibrahim , Almutairi Mashael , Althubaiti Manal , Alsehli Faisal , Alwatban Ahmed , Alsaif Saif
Published: July 2024

Objective

To explore the relationship between Oxygenation Index (OI) and Oxygen Saturation Index (OSI) among infants with Congenital Diaphragmatic Hernia (CDH), both within the first 24 h after birth and in extended observations in those who survived until their surgical intervention.

Methods

Seven- years retrospective review of CDH cases at a single Level III neonatal intensive care unit. The correlations of various combinations of OI-OSI pairs were assessed using the Spearman's rho Correlation Coefficient. Additionally, during the initial 24 h, the correlations between admission (first), best (lowest), highest, and mean OI and OSI values were determined. The predictive ability of the first 24 h oxygen and oxygen saturation indices for mortality and other adverse outcomes were assessed using the Area Under the Curve (AUC) analysis.

Results

Thirty-seven infants with CDH were included in the analysis. A strong correlation was observed between all pairs of OI/OSI (2,289) (Spearman's rho = 0.843), matched pairs of Postductal OI/OSI (1,232 pairs) (Spearman's rho = 0.835) and the unmatched pairs of Postductal OI and Preductal OSI (1,057 pairs) (Spearman's rho = 0.852). Using the regression equations for all pairs, matched and unmatched OI/OSI pairs, we deduced that for clinically pertinent OI thresholds of 10, 15, 20 and 40, the corresponding OSI values were 5, 8, 11, and 23, respectively. Furthermore, in the first 24 h, strong correlations were evident between OI/OSI: at admission (Spearman's rho = 0.783), best OI/OSI (Spearman's rho = 0.848), and highest OI/OSI (Spearman's rho = 0.921). The most robust correlation was observed between the mean OI/OSI with a Spearman's rho of 0.928. First (AUC = 0.849), best (AUC = 0.927), highest (AUC = 0.942) and mean day 1 OI (AUC = 0.946) were all predictive of mortality. Similarly, first (AUC = 1.00), best (AUC = 0.989), highest (AUC = 1.00) and the mean OSI in day 1 (AUC = 0.978) were all predictive of mortality. All of the OIs and OSIs in day 1 except for the admission OSI (AUC = 0.683) were predictive of pulmonary hypertension. Additionally, all of OI and OSI indices in the first 24-hour except for the best day 1 OI (AUC = 0.674) were predictive of the need for rescue HFOV.

Conclusion

There were a strong correlation between the OI and OSI in infants with CDH. Oxygenation indices and OSI in the first 24 h were predictive of mortality and other adverse outcomes in infants with CDH.
Development and validation of a prognostic nomogram to predict 30-day all-cause mortality in patients with CRO infection treated with colistin sulfate
Authors: Li Wei , Liu Yu , Xiao Lu , Cai Xuezhou , Gao Weixi , Xu Dong , Han Shishi , He Yan
Published: July 2024

Background

Carbapenem-resistant Gram-negative organism (CRO) infection is a critical clinical disease with high mortality rates. The 30-day mortality rate following antibiotic treatment serves as a benchmark for assessing the quality of care. Colistin sulfate is currently considered the last resort therapy against infections caused by CRO. Nevertheless, there is a scarcity of reliable tools for personalized prognosis of CRO infections. This study aimed to develop and validate a nomogram to predict the 30-day all-cause mortality in patients with CRO infection who underwent colistin sulfate treatment.

Methods

A prediction model was developed and preliminarily validated using CRO-infected patients treated with colistin sulfate at Tongji Hospital in Wuhan, China, who were hospitalized between May 2018 and May 2023, forming the study cohort. Patients admitted to Xianning Central Hospital in Xianning, China, between May 2018 and May 2023 were considered for external validation. Multivariate logistic regression was performed to identify independent predictors and establish a nomogram to predict the occurrence of 30-day all-cause mortality. The receiver operating characteristic (ROC) curve, the area under the ROC curve (AUC), and the calibration curve were used to evaluate model performance. The decision curve analysis (DCA) was used to assess the model clinical utility.

Results

A total of 170 patients in the study cohort and 65 patients in the external validation cohort were included. Factors such as age, duration of combination therapy, nasogastric tube placement, history of previous surgery, presence of polymicrobial infections, and occurrence of septic shock were independently associated with 30-day all-cause mortality and were used to construct the nomogram. The AUC of the nomogram constructed from the above six factors was 0.888 in the training set. The Hosmer-Lemeshow test showed that the model was a good fit (p = 0.944). The calibration curve of the nomogram was close to the ideal diagonal line. Furthermore, the decision curve analysis demonstrated significantly better net benefit in the model. The external validation proved the reliability of the prediction nomogram.

Conclusion

A nomogram was developed and validated to predict the occurrence of 30-day all-cause mortality in patients with CRO infection treated with colistin sulfate. This nomogram offers healthcare providers a precise and efficient means for early prediction, treatment management, and patient notification in cases of CRO infection treated with colistin sulfate.
THSD7A-associated membranous nephropathy involves both complement-mediated and autonomous podocyte injury
Authors: Liu Jing , Malhotra Deepak , Ge Yan , Gunning William , Dworkin Lance , Gong Rujun
Published: July 2024

Membranous nephropathy (MN) continues to be a leading cause of nephrotic syndrome in non-diabetic adults. As a unique subtype in the serology-based classification of MN, thrombospondin type 1 domain containing 7A (THSD7A)-associated MN has attracted increasing interest, because, unlike other autoantigens, THSD7A is also expressed in preclinical species, facilitating the study of its role in MN. A heterologous mouse model of THSD7A-associated MN was previously established using a proprietary in-house antibody that was unfortunately not available to the research community. Here, we developed a mouse model of THSD7A-associated MN by administering a commercially available antibody targeting the most N-terminal part of THSD7A. Our model was characterized by heavy proteinuria and pathological features of human MN without sex differences. Complement depletion with cobra venom factor only partially attenuated proteinuria and glomerular injury in this model, entailing that complement-independent pathomechanisms also contribute. Consistently, in vitro in primary podocytes, exposure to the anti-THSD7A antibody caused evident podocytopathic changes, including disruption of actin cytoskeleton integrity, podocyte hypermobility, oxidative stress, and apoptotic cell death. These signs of podocytopathy were preserved, albeit to a lesser extent, after complement inactivation, indicating autonomous podocyte injury. Furthermore, as the first FDA-approved treatment for primary MN, adrenocorticotropic hormone therapy with repository corticotropin injection (Purified Cortrophin Gel®) appeared to be beneficial and significantly attenuated proteinuria and glomerular injury, suggesting that this model may be useful for developing novel treatments or understanding the pathogenesis of MN. Collectively, our model, based on the use of a commercially available anti-THSD7A antibody, will be an important tool for MN research.
Study on the change of global ecological distribution of Nicotiana tabacum L. based on MaxEnt model
Authors: Jia Linxi , Sun Mingming , He Mingrui , Yang Mingfeng , Zhang Meng , Yu Hua
Published: July 2024

Nicotiana tabacum L. (tobacco) has extremely high economic value, medicinal value, scientific research value and some other uses. Though it has been widely cultivated throughout the world, classification and change of its suitable habitats is not that clear, especially in the context of global warming. In order to achieve rational cultivation and sustainable development of tobacco, current (average from 1970-2000) and future (2070, average from 2061-2080) potential suitable habitats of Nicotiana tabacum L. were forecasted with MaxEnt model and ArcGIS platform based on 854 occurrence data and 22 environmental factors in this study. The results revealed that mean temperature of warmest quarter (bio10), annual precipitation (bio12), solar radiation in September (Srad9), and clay content (CLAY) were the four decisive environment variables for the distribution of Nicotiana tabacum L. Under current climate conditions, suitable habitats of Nicotiana tabacum L. were mainly distributed in south-central Europe, south-central North America, most parts of South America, central Africa, south and southeast Asia, and southeast coast of Australia, and only 13.7% of these areas were highly suitable. By the year 2070, suitable habitats under SSP1-2.6, SSP3-7.0, and SSP5-8.5 climate scenarios would all increase with the largest increase found under SSP3-7.0 scenario, while suitable habitats would reduce under SSP2-4.5 climate scenario. Globally, the center of mass of suitable habitats would migrate to southeast to varying degrees within Libya under four different climate scenarios. The emergence of new habitats and the disappearance of old habitats would all occur simultaneously under each climate scenario, and the specific changes in each area, combined with the prediction results under current climate conditions, will provide an important reference for the adjustment of agronomic practices and rational cultivation of Nicotiana tabacum L. both currently and in the future.
Potassium silica nanostructure improved growth and nutrient uptake of sorghum plants subjected to drought stress
Authors: Alharbi Khadiga , Alnusairi Ghalia S. H. , Alnusaire Taghreed S. , Alghanem Suliman M. S. , Alsudays Ibtisam Mohammed , Alaklabi Abdullah , Soliman Mona H.
Published: July 2024

Introduction

Recent advancements in nanotechnology present promising opportunities for enhancing crop resilience in adverse environmental conditions.

Methods

In this study, we conducted a factorial experiment to investigate the influence of potassium nanosilicate (PNS) on sorghum plants exposed to varying degrees of drought stress A randomized complete block design with three replications was employed to subject the sorghum plants to different drought conditions. The three levels of stress were designated as non-stress (NS at -0.03 MPa), moderate stress (MD at -0.6 MPa), and severe stress (SD at -1.2 MPa). The plants were administered PNS at concentrations of 0 mM (control), 3.6 mM Si, and 7.2 mM Si.

Results and discussion

As drought stress intensified, we observed significant reductions in multiple plant parameters, including height, fresh weight, dry weight, leaf number, stem diameter, cluster length, seed weight, and nutrient uptake, with the most pronounced effects observed under SD conditions. Interestingly, nitrogen (N) and potassium (K) levels exhibited an increase under drought stress and PNS application, peaking at MD, alongside Si concentrations. Notably, PNS application facilitated enhanced nutrient uptake, particularly evident in the significant increase in nitrogen concentration observed at 3.6 mM PNS. Furthermore, the application of PNS significantly enhanced the fresh weight and nutrient concentrations (notably K and Si) in sorghum seeds under drought stress, despite varying statistical significance for other nutrients. These findings shed light on the mechanisms through which PNS exerts beneficial effects on plant performance under drought stress. By elucidating the complex interactions between PNS application, drought stress, and plant physiology, this study contributes significantly to the development of sustainable agricultural practices aimed at bolstering crop resilience and productivity in water-limited environments.
Overcoming treatment-resistant depression with machine-learning based tools: a study protocol combining EEG and clinical data to personalize glutamatergic and brain stimulation interventions (SelecTool Project)
Authors: Pettorruso Mauro , Di Lorenzo Giorgio , Benatti Beatrice , d’Andrea Giacomo , Cavallotto Clara , Carullo Rosalba , Mancusi Gianluca , Di Marco Ornella , Mammarella Giovanna , D’Attilio Antonio , Barlocci Elisabetta , Rosa Ilenia , Cocco Alessio , Padula Lorenzo Pio , Bubbico Giovanna , Perrucci Mauro Gianni , Guidotti Roberto , D’Andrea Antea , Marzetti Laura , Zoratto Francesca , Dell’Osso Bernardo Maria , Martinotti Giovanni
Published: July 2024

Treatment-Resistant Depression (TRD) poses a substantial health and economic challenge, persisting as a major concern despite decades of extensive research into novel treatment modalities. The considerable heterogeneity in TRD’s clinical manifestations and neurobiological bases has complicated efforts toward effective interventions. Recognizing the need for precise biomarkers to guide treatment choices in TRD, herein we introduce the SelecTool Project. This initiative focuses on developing (WorkPlane 1/WP1) and conducting preliminary validation (WorkPlane 2/WP2) of a computational tool (SelecTool) that integrates clinical data, neurophysiological (EEG) and peripheral (blood sample) biomarkers through a machine-learning framework designed to optimize TRD treatment protocols. The SelecTool project aims to enhance clinical decision-making by enabling the selection of personalized interventions. It leverages multi-modal data analysis to navigate treatment choices towards two validated therapeutic options for TRD: esketamine nasal spray (ESK-NS) and accelerated repetitive Transcranial Magnetic Stimulation (arTMS). In WP1, 100 subjects with TRD will be randomized to receive either ESK-NS or arTMS, with comprehensive evaluations encompassing neurophysiological (EEG), clinical (psychometric scales), and peripheral (blood samples) assessments both at baseline (T0) and one month post-treatment initiation (T1). WP2 will utilize the data collected in WP1 to train the SelecTool algorithm, followed by its application in a second, out-of-sample cohort of 20 TRD subjects, assigning treatments based on the tool’s recommendations. Ultimately, this research seeks to revolutionize the treatment of TRD by employing advanced machine learning strategies and thorough data analysis, aimed at unraveling the complex neurobiological landscape of depression. This effort is expected to provide pivotal insights that will promote the development of more effective and individually tailored treatment strategies, thus addressing a significant void in current TRD management and potentially reducing its profound societal and economic burdens.
Fostering cultural resilience: assessing the success of a locally engaged and adapted mental health intervention in Gaza
Authors: Rockowitz Sarah , Bayoumi Rasha , Parr Nora , Awad Abdullah , Altawil Mohamed , Elmusharaf Khalifa
Published: July 2024

In ongoing-conflict-affected regions like Gaza, the prevalence of complex and intersecting post-traumatic stress disorders (PTSDs) necessitates innovative interventions. Our study explores a mental health care approach that has been culturally adapted for 15 years to address the complex landscape of PTSD in the Gaza Strip. Tarkiz was initially developed as ‘Focusing’, a metacognitive approach founded by Eugene Gendlin in 1950s Chicago. Tarkiz has been iteratively adapted and implemented for over a decade in Gaza by a team of local practitioners. The program’s unique emphasis lies in its engagement approach, which relies on community participation and partnership building. The aim of this study was to qualitatively explore the perceived success of the program from the perspectives of the practitioners who adapted and delivered the program and the clients who participated in it. Data collection was driven by a multicultural research team, and prioritized capacity-building opportunities for Palestinian practitioners who lead on the development of research questions defining success. This exemplifies a successful science diplomacy model, emphasizing a collaborative approach, cultural sensitivity, and adaptable partnerships essential in global public health.
The mediating effect of e-health literacy on social support and behavioral decision-making on glycemic management in pregnant women with gestational diabetes: a cross-sectional study
Authors: Yumei Peng , Huiying Ke , Liqin Shen , Xiaoshan Zhao , Meijing Zhao , Yaping Xie , Huifen Zhao
Published: July 2024

Background

Social support and e-health literacy are closely related to individual health behaviors, while behavior is premised on decision-making. Few studies have identified the relationships among social support, e-health literacy, and behavioral decision-making, and the nature of these relationships among pregnant women with gestational diabetes remains unclear. Therefore, this study aimed to investigate relationships among social support, e-health literacy, and glycemic management behavioral decisions in pregnant women with gestational diabetes.

Methods

Using continuous sampling, an online cross-sectional survey was conducted among pregnant women with gestational diabetes who met the inclusion and exclusion criteria at four Class 3 hospitals in Fujian Province from October to December 2023. A structured questionnaire was used to collect data on general characteristics, socioeconomic status, social support, e-health literacy, and behavioral decision-making regarding glycemic management. Descriptive statistical analyses, correlation analyses, and mediation effects were used to assess associations.

Results

A total of 219 pregnant women with gestational diabetes participated, and 217 valid results were obtained. The level of glycemic management behavior decision-making in women with gestational diabetes was positively correlated with e-health literacy (r = 0.741, p < 0.01) and with perceived social support (r = 0.755, p < 0.01). E-health literacy was positively correlated with perceived social support (r = 0.694, p < 0.01). The indirect effect of perceived social support on glycemic management behavior decisions through e-health literacy (a*b) was 0.153, accounting for 38% of the total effect.

Conclusion

Social support and e-health literacy in pregnant women with gestational diabetes are related to behavioral decision-making in glycemic management. The results of this study provide a reference for developing targeted measures to improve glycemic management behaviors in pregnant women with gestational diabetes, which is crucial for achieving sustainable glycemic management.
Facilitators and barriers in interprofessional collaboration around physical activity on prescription—a focus group study in a Swedish school setting
Authors: Wiklund Emelie , Vikman Jenny , Wiklund Maria , Hedenborg Susanna
Published: July 2024

In Swedish school health services, local initiatives have been taken to use physical activity on prescription (PAP) to encourage physically inactive children to become more active. Previous research shows that interprofessional collaboration plays a crucial role in promoting physical activity in children, as well as in promoting health in schools. However, there is a lack of knowledge about PAP for children in the school setting, including how medical and educational staff can work together to encourage children who have been recommended PAP. Therefore, this study aims to explore the perceived facilitators and barriers concerning interprofessional collaboration regarding physical activity on prescription in the school setting, as viewed from the professionals’ perspectives. Semi-structured interviews were conducted with 21 professionals who work with the method in school settings. The data were analyzed using Reflexive Thematic Analysis. The results reveal both barriers and facilitators for interprofessional collaboration on PAP in the school setting, as perceived by professionals. Organizational and structural obstacles within school institutions hinder collaboration, while a shared commitment to PAP, characterized by consensus-building, acts as a facilitating factor. PAP for children in a school setting is still an unexplored area and further research is required.
Kansas agriculture in 2050: a pathway for climate-resilient crop production
Authors: Suttles Kelly M. , Smoliak Brian V. , Ranade Aditya P. , Potter Samuel F. , Jaeger Marika , McLellan Eileen L.
Published: July 2024

Introduction

Advances in farming technologies and practices have helped farmers to increase crop yields, but continued production increases – while needed to feed a growing global population – are being hindered by climate change and other environmental challenges. Kansas, a globally important region for wheat production, is already experiencing threats to staple crop production. This study explores one possible alternative future for Kansas crop production in which farmers could, by crop switching, continue to grow nutrient-rich crops while responding to hotter temperatures and increasing water demand.

Methods

We used a combination of climate and crop modeling with simple water budgets to identify optimal crop mixes under anticipated climatic and water constraints. Interviews with Kansas farmers helped identify feasible crop switching options: sorghum instead of corn (maize), winter rye and winter oats instead of winter wheat, and millet instead of soybeans.

Results

Our analysis suggests that a sizeable proportion of current Kansas cropland would need to shift to these alternative crops by 2050 to meet anticipated water constraints and produce equivalent nutritional value under projected climate conditions. Alternative crops could increase from 16% of Kansas' crop area in 2021 to 43% of the area in 2050, resulting in a reduction in 2050 crop water demand of 12% relative to that of the current crop mix. This crop water demand reduction would be concentrated in parts of the state that will experience the greatest change in water needs between today and mid-century due to changing climate conditions.

Discussion

Our analysis shows that, by changing (diversifying) the mix of crops grown, it is biophysically possible for crop production in Kansas to be both sustainable and resilient under future climate conditions. However, achieving a more climate-resilient crop mix on the ground, in Kansas and elsewhere, will require major shifts in the broader agricultural system. Food companies, agricultural lenders, and policymakers can play a key role in enabling farmers to adapt cropping systems in the face of climate and environmental challenges.
Unleashing the potential of cell painting assays for compound activities and hazards prediction
Authors: Odje Floriane , Meijer David , von Coburg Elena , van der Hooft Justin J. J. , Dunst Sebastian , Medema Marnix H. , Volkamer Andrea
Published: July 2024

The cell painting (CP) assay has emerged as a potent imaging-based high-throughput phenotypic profiling (HTPP) tool that provides comprehensive input data for in silico prediction of compound activities and potential hazards in drug discovery and toxicology. CP enables the rapid, multiplexed investigation of various molecular mechanisms for thousands of compounds at the single-cell level. The resulting large volumes of image data provide great opportunities but also pose challenges to image and data analysis routines as well as property prediction models. This review addresses the integration of CP-based phenotypic data together with or in substitute of structural information from compounds into machine (ML) and deep learning (DL) models to predict compound activities for various human-relevant disease endpoints and to identify the underlying modes-of-action (MoA) while avoiding unnecessary animal testing. The successful application of CP in combination with powerful ML/DL models promises further advances in understanding compound responses of cells guiding therapeutic development and risk assessment. Therefore, this review highlights the importance of unlocking the potential of CP assays when combined with molecular fingerprints for compound evaluation and discusses the current challenges that are associated with this approach.
Implications of hypocobalaminemia as a negative prognostic marker in juvenile dogs with parvovirus enteritis
Authors: Luckschander-Zeller Nicole , Giani Bettina , Doulidis Pavlos G. , Plickert Hanna D. , Tichy Alexander , Marculescu Rodrig , Schwendenwein Ilse , Burgener Iwan A.
Published: July 2024

Introduction

Canine Parvovirus 2 (CPV-2) infection poses a significant global health risk to susceptible dogs. Hypocobalaminemia, defined as reduced serum cobalamin (CBL) concentrations, is a recognized complication in chronic enteropathies in adult dogs but remains poorly understood in the context of acute enteropathies, especially in young dogs. The aim of this study was to investigate the frequency and severity of hypocobalaminemia in young dogs with parvovirus enteritis and evaluation of CBL as a predictor of outcome.

Materials and methods

Thirty client-owned dogs diagnosed with parvovirus infection and thirty healthy controls were enrolled. Clinical, hematological, and biochemical tests, including CBL and serum methylmalonic acid (MMA) concentrations, were assessed.

Results

Results indicated a significantly higher prevalence of hypocobalaminemia in dogs with parvovirus enteritis compared to healthy controls, as well as a significant correlation with a disease severity score. Moreover, survivors demonstrated higher CBL concentrations than non-survivors, suggesting an eventual prognostic value of CBL status. However, parenteral CBL supplementation showed no significant effect on serum CBL or MMA concentrations, highlighting potential challenges in CBL uptake at the cellular level.

Discussion

Hypocobalaminemia in this population is caused by multiple factors such as reduced nutritional absorption, gastrointestinal losses, and increased metabolic demands. Further research is needed to develop tailored management strategies, evaluate the effectiveness of CBL supplementation, and understand the mechanisms behind hypocobalaminemia in parvovirus infection.
Development and characterization of an immortalized nasopharyngeal epithelial cell line to explore airway physiology and pathology in yak (Bos grunniens)
Authors: Qi Jiancheng , Zhang Jizong , Huang Fangyuan , Xie Yue , Guo Hongrui , Gou Liping , Zuo Zhicai , Fang Jing
Published: July 2024

Airway epithelial cells play a crucial role in investigating the physiological and pathological mechanisms of the respiratory tract in yaks, a species whose unique respiratory system has garnered extensive interest. Despite this growing interest, there currently are no available airway epithelial cell lines from yaks, underscoring the crucial need to establish a yak respiratory epithelial cell line. Therefore, our objective was to isolate a population of primary yak nasopharyngeal epithelial cells (pYNE) and transform them into immortalized yak nasopharyngeal epithelial cells (iYNE), assessing their suitability as an in vitro model. Employing a combined method of physical elimination and differential adhesion, we successfully isolated a population of high-purity pYNE, and developed an iYNE line through pCI-neo-hTERT plasmid transfection. Karyotype and transmission electron microscopy analyses confirmed that pYNE and iYNE share identical morphologies and structures. Gel electrophoresis and real-time PCR analyses demonstrated that pYNE and iYNE expressed similar levels of KRT18 and CDH1 genes (p ≥ 0.541). Notably, iYNE expressed a significantly high level of TERT gene expression (p < 0.001). Immunofluorescence analysis demonstrated that both cell types expressed Pan-Cytokeratin, ZO-1, and E-cadherin proteins. Furthermore, immunoblotting analysis indicated significantly higher levels of hTERT and Ki67 proteins in iYNE (p < 0.001), and similar levels of Cluadin-3 and Occludin proteins (p ≥ 0.103). Proliferation curve analysis highlighted iYNE’s serum-dependency and significantly enhanced proliferation capacities (p < 0.001). Additionally, pYNE and iYNE cells demonstrated comparable susceptibilities to infectious bovine rhinotracheitis virus (IBRV). These findings collectively suggest that the developed iYNE retains the evaluated physiological characteristics of pYNE, making it an appropriate in vitro model. This advancement will facilitate further investigation into the respiratory physiological and pathological mechanisms in yaks.
Dietary supplementation with a novel acidifier sodium diformate improves growth performance by increasing growth-related hormones levels and prevents Salmonella enterica serovar Pullorum infection in chickens
Authors: Sun Yufan , Zhang Xiaofen , Han Weiyao , Liao Weilian , Huang Jing , Chen Yong , Li Hengzhang , Chen Xiabing , Huang Qi , Zhou Rui , Li Lu
Published: July 2024

Since the use of antibiotics as growth promoters in animal feed has been restricted or banned in several countries, finding suitable alternatives is crucial for maintaining animal health. In this study, a novel formate acidifier named sodium diformate (NaDF) was synthesized, and the effects on growth performance and the prevention effects against Salmonella enterica serovar Pullorum infections in chickens were assessed. In broilers, NaDF supplementation improved growth performance, as evidenced by increased body weights and reduced feed conversion ratios. At 38 days of age, NaDF supplementation increased the levels of growth-hormone and ghrelin in the serum, lowered pH values in the gut, improved duodenal morphology, as shown by increased villus length/crypt depth ratios. NaDF also modulated the abundance of beneficial and harmful bacteria without changing the general microbiota diversity and short-chain fatty acids levels, which would be beneficial for maintaining gut homeostasis during its use. NaDF exhibited a broad spectrum of antibacterial activity in vitro. Supplementation with NaDF effectively decreased S. Pullorum colonization in the cecum, liver and spleen in chickens, and mitigated pathological changes in the tissues. Therefore, as a novel acidifier, NaDF can improve chicken growth performance by increasing growth-related hormones levels while maintaining the diversity of gut microbiota, and also resist intestinal bacterial infection. These results provided evidences for the application of NaDF as an effective and safe animal feed in poultry farming.
The Sliding Mode Control for Piezoelectric Tip/Tilt Platform on Precision Motion Tracking
Authors: Zeng Xianfeng , Zhang Xiaozhi , Nan Feng
Published: July 2024

This paper presents the design of a sliding mode controller to compensate hysteresis nonlinearity and achieve precision motion tracking for a novel piezoelectric tip/tilt platform driven by a PZT actuator. The sliding mode control scheme is based on the unique physical characteristics of the piezoelectric tip/tilt platform. The proposed scheme effectively guides the platform state onto a predefined sliding surface and ensures its sustained movement along this manifold. This approach reduces tracking errors compared to conventional methodologies. The stability of the sliding mode control scheme is demonstrated by the Lyapunov theory framework. It achieves precise motion control with minimal tracking error on a piezoelectric tip/tilt platform. The properties of the controller have been confirmed through experimental tests. The proposed control scheme enhances the robust tracking and stability performance on the piezoelectric tip/tilt platform, outperforming traditional control schemes. Compared with the P562.6CD produced by PI Germany, the proposed innovative approach not only boosts the platform’s resolution by 32% but also implements a 33% reduction in cost.
Air Traffic Control Speech Enhancement Method Based on Improved DNN-IRM
Authors: Wu Yuezhou , Li Pengfei , Zhang Siling
Published: July 2024

The quality of air traffic control speech is crucial. However, internal and external noise can impact air traffic control speech quality. Clear speech instructions and feedback help optimize flight processes and responses to emergencies. The traditional speech enhancement method based on a deep neural network and ideal ratio mask (DNN-IRM) is prone to distortion of the target speech in a strong noise environment. This paper introduces an air traffic control speech enhancement method based on an improved DNN-IRM. It employs LeakyReLU as an activation function to alleviate the gradient vanishing problem, improves the DNN network structure to enhance the IRM estimation capability, and adjusts the IRM weights to reduce noise interference in the target speech. The experimental results show that, compared with other methods, this method improves the perceptual evaluation of speech quality (PESQ), short-term objective intelligibility (STOI), scale-invariant signal-to-noise ratio (SI-SNR), and speech spectrogram clarity. In addition, we use this method to enhance real air traffic control speech, and the speech quality is also improved.
A Method for Air Route Network Planning of Urban Air Mobility
Authors: Li Jie , Shen Di , Yu Fuping , Qi Duo
Published: July 2024

Urban air mobility is an effective solution to address the current issue of ground traffic congestion in future cities. However, as the user scale continues to expand, the current civil aviation flight scheduling and control methods are becoming inadequate to meet the high-volume flight guarantee demands of future urban air transportation. In order to effectively handle and resolve potential issues in this field in the future, this paper proposes a method for planning urban air mobility route networks. The planning process is divided into two stages: construction and optimization. Methods for constructing urban air mobility route networks based on flight routes and global optimization methods based on node movement are proposed in each stage. In the construction stage, a complete construction process is designed to generate routes based on existing flight routes, in line with the trend of urban air transportation development. In the optimization stage, inspired by the ant colony algorithm, node transfer rules and information transfer rules are incorporated to design a global optimization process and algorithm for route networks. Experimental results demonstrate the effectiveness and advancement of the proposed planning method.
Airfoil Design Optimization of Blended Wing Body for Various Aerodynamic and Stealth Stations
Authors: Zhang Wei , Zhou Lin , Zhao Ke , Zhang Ruibin , Gao Zhenghong , Shu Bowen
Published: July 2024

The airfoil is the foundation of an aircraft, and its characteristics have a significant impact on those of the aircraft. Conventional airfoil design mainly focuses on improving aerodynamic performance, while flying wing airfoil designs should also consider layout stability and stealth performance. The design requirements for an airfoil vary with its position on the flying wing layout aircraft based on corresponding spanwise flow field characteristics. By analyzing the spanwise flow characteristics of the flying wing, partition design models for flying wing airfoils were established in this study, and a series of flying wing airfoil designs that consider aerodynamics and aerodynamic/stealth were implemented. Then, the designed airfoils were configured on a three-dimensional X-47B layout for testing and verification. The results showed that the aerodynamic design and the aerodynamic/stealth design exhibited significant improvements in terms for aerodynamic and longitudinal trimming characteristics. However, the cruise drag performance of the aerodynamic/stealth design was slightly worse than that of the aerodynamic design, although the longitudinal moment trimming characteristics were basically the same. The stealth characteristics of the aerodynamic/stealth design had significant advantages, indicating that there were weak contradictions between the aerodynamic, stealth, and trimming requirements in the design of the flying wing.
Effect of Magnetic Field and UV-C Radiation on Postharvest Fruit Properties
Authors: Gąstoł Maciej , Błaszczyk Urszula
Published: July 2024

This review focuses on the recent information on the effect of different types of magnetic fields (MFs) and ultraviolet radiation (UV-C) on the processes that may finally affect fruit quality and its storage potential. Firstly, the biological effect of MFs on every plant’s growth and development level is described. The magnetic field interacts with a plant’s metabolism and changes the permeability of membranes affecting cells’ homeostasis. It also could affect early seedling development, stimulating enzyme activity and protein synthesis, and later on nutrient and water uptake of adult plants. In some cases, it makes plants more resilient, increasing their tolerance to environmental stresses. Also, MF treatment could lower the disease index of plants, thus improving the internal and external fruit quality indices. The second part of this review focuses on interesting perspectives of using UV-C radiation to reduce postharvest fruit diseases, but also to delay fruit ripening and senescence. The application of UV-C light to combat postharvest infections is associated with two mechanisms of action, such as direct elimination of microorganisms located on the fruit surface and indirect triggering of the plant’s defense reaction. Moreover, the use of hormetic doses of UV-C can additionally increase the nutritional properties of fresh fruit, lead to the accumulation of desired phytochemicals such as polyphenols, for example, to increase anthocyanin or resveratrol content, or elevate antioxidant activity.
Evidence of Cooperative Interactions between Rhizobacteria and Wood-Decaying Fungi and Their Effects on Maize Germination and Growth
Authors: Rocha Ricardo , Venâncio Cátia , Cardoso Paulo , Lourenço João , Figueira Etelvina
Published: July 2024

Advances in soil microbial communities are driving agricultural practices towards ecological sustainability and productivity, with engineering microbial communities significantly contributing to sustainable agriculture. This study explored the combined effects of two white-rot fungi (Trametes sp. and Pleurotus sp.) and six rhizobacterial strains belonging to four genera (Acinetobacter sp., Enterobacter sp., Flavobacterium sp., and Pseudomonas sp.) on maize growth and soil enzymatic activity over a 14-day period. At the plant level, germination, fresh and dry mass of the aerial and root parts, length, and stage of development of the stem, as well as the chlorophyll content, were evaluated. Furthermore, soil dehydrogenase, acid and alkaline phosphatases, pH, and electrical conductivity were evaluated. Rot fungi induced distinct effects on maize germination, with Pleurotus sp. strongly suppressing maize germination by 40% relative to that of the control. The isolated bacterial strains, except Enterobacter sp. O8, and 8 of the 12 fungus + bacterial strain combinations induced germination rates higher than those of the control (≥40%). Combinations of Flavobacterium sp. I57 and Pseudomonas sp. O81 with the rot fungus Pleurotus sp. significantly improved plant shoot length (from 28.0 to 37.0 cm) and developmental stage (fourth leaf length increase from 10.0 to 16.8 cm), respectively, compared with the same bacteria alone or in combination with the rot fungus Trametes sp. In the soil, the presence of both fungi appeared to stabilize phosphatase activity compared to their activity when only bacteria were present, while also promoting overall dehydrogenase enzymatic activity in the soil. Integrating all parameters, Trametes sp. rot fungus + Enterobacter sp. O8 may be a potential combination to be explored in the context of agricultural production, and future studies should focus on the consistency of this combination’s performance over time and its effectiveness in the field.