bluuebunny/crossref_metadata_2025 huggingface dataset:
Instability and Stasis Among the Microbiome of Seagrass Leaves, Roots and Rhizomes, and Nearby Sediments Within a Natural pH Gradient
Authors: Banister Raymond B. , Schwarz Melbert T. , Fine Maoz , Ritchie Kim B. , Muller Erinn M.
Published: October 2021

Seagrass meadows are hotspots of biodiversity with considerable economic and ecological value. The health of seagrass ecosystems is influenced in part by the makeup and stability of their microbiome, but microbiome composition can be sensitive to environmental change such as nutrient availability, elevated temperatures, and reduced pH. The objective of the present study was to characterize the bacterial community of the leaves, bulk samples of roots and rhizomes, and proximal sediment of the seagrass species Cymodocea nodosa along the natural pH gradient of Levante Bay, Vulcano Island, Italy. The bacterial community was determined by characterizing the 16S rRNA amplicon sequencing and analyzing the operational taxonomic unit classification of bacterial DNA within samples. Statistical analyses were used to explore how life-long exposure to different pH/pCO2 conditions may be associated with significant differences in microbial communities, dominant bacterial classes, and microbial diversity within each plant section and sediment. The microbiome of C. nodosa significantly differed among all sample types and site-specific differences were detected within sediment and root/rhizome microbial communities, but not the leaves. These results show that C. nodosa leaves have a consistent microbial community even across a pH range of 8.15 to 6.05. The ability for C. nodosa to regulate and maintain microbial structure may indicate a semblance of resilience within these vital ecosystems under projected changes in environmental conditions such as ocean acidification.
Individual values and spirituality and their meaning for affective well-being and engagement with life in very old age
Authors: Reissmann Marcella , Storms Anna , Woopen Christiane
Published: October 2021

Background

Individuals’ ideals and aspirations are considered to constitute a central reference frame for subjective evaluations of their perceived reality, and, thus, to be crucial for individual quality of life (QoL) outcomes. By examining individual values and spirituality in very old people, the aim of this study was to describe two constructs representing the aspirations of the individual, as well as the relation of these constructs to both hedonic and eudaimonic QoL outcomes in very old age (VOA).

Material and methods

Cross-sectional data from a representative survey of people in VOA (NRW80+, n = 1863) were used. Individual values were assessed based on the Portrait Value Questionnaire. A revised questionnaire was developed drawing on the Spiritual Health and Life-Orientation Measure. Individual values and spirituality were studied using descriptive statistics, and hierarchical linear regression models were used to analyze their predictive value for two QoL outcomes: 1) affective well-being as an indicator of hedonic QoL, which was assessed using the positive affect subscale of the short form of the Positive and Negative Affect Schedule, and 2) engagement with life, which captures eudaimonic aspects and which was measured with a subscale of the Valuation of Life Scale.

Results

The most important values were both protection and growth-oriented values with a social focus. However, only values representing strivings for growth had a positive association with QoL outcomes. Spirituality was of high relevance to very old people, although not in the sense of religious institutions or practices. Rather, it predominantly consisted in environmental, interpersonal, and transcendental connectedness, all of which were positively connected to QoL outcomes.

Conclusion

Individual values and spirituality can be an important resource for hedonic as well as eudaimonic QoL; however, age-related losses may lead to an emphasis of protective values that are not beneficial in terms of QoL. To support older people on their spiritual journey, a broad concept of spirituality needs to be established among researchers as well as practitioners.
Fetal ciliopathies: a retrospective observational single-center study
Authors: Simonini Corinna , Floeck Anne , Strizek Brigitte , Mueller Andreas , Gembruch Ulrich , Geipel Annegret
Published: October 2021

Purpose

Report on the diagnosis of prenatally suspected multisystem ciliopathies in a single center between 2002 and 2020.

Methods

Retrospective observational single-center study including pregnancies with prenatal ultrasound features of multisystem ciliopathies, such as hyperechogenic kidneys together with polydactyly and/or other skeletal and extraskeletal findings. Cases were compared according to their prenatal findings and outcomes.

Results

36 cases of multisystem ciliopathies were diagnosed. Meckel-Gruber syndrome (MKS) was the most common ciliopathy (n = 19/36, 52.8%), followed by disorders that belong to the group of short-rib thoracic dysplasia (SRTD, n = 10/36, 27.8%) McKusick–Kaufmann syndrome (MKKS, n = 4/36, 11.1%), Bardet–Biedl syndrome (BBS, n = 2/36, 5.5%) and Joubert syndrome (n = 1/36, 2.8%). All cases showed abnormalities of the kidneys, most often hyperechogenic parenchyma (n = 26/36, 72.2%), cystic dysplasia (n = 24/36, 66.7%), and/or bilateral kidney enlargement (n = 22/36, 61.1%). Oligohydramnios was mainly present in fetuses with MKS. Polydactyly (n = 18/36), abnormalities of the CNS (n = 25/36), and heart defects (n = 10/36) were associated in 50%, 69.4%, and 27.8%, respectively.

Conclusion

Prenatal detection of renal abnormalities associated with skeletal or brain abnormalities should raise the suspicion for multisystem ciliopathies. Prenatal ultrasound can help to differentiate between different diseases and pave the way for subsequent targeted genetic testing.
Music perception and speech intelligibility in noise performance by Italian-speaking cochlear implant users
Authors: Dincer D’Alessandro Hilal , Boyle Patrick J. , Portanova Ginevra , Mancini Patrizia
Published: October 2021

Objective

The goal of this study was to investigate the performance correlations between music perception and speech intelligibility in noise by Italian-speaking cochlear implant (CI) users.

Materials and methods

Twenty postlingually deafened adults with unilateral CIs (mean age 65 years, range 46–92 years) were tested with a music quality questionnaire using three passages of music from Classical Music, Jazz, and Soul. Speech recognition in noise was assessed using two newly developed adaptive tests in Italian: The Sentence Test with Adaptive Randomized Roving levels (STARR) and Matrix tests.

Results

Median quality ratings for Classical, Jazz and Soul music were 63%, 58% and 58%, respectively. Median SRTs for the STARR and Matrix tests were 14.3 dB and 7.6 dB, respectively. STARR performance was significantly correlated with Classical music ratings (rs = − 0.49, p = 0.029), whereas Matrix performance was significantly correlated with both Classical (rs = − 0.48, p = 0.031) and Jazz music ratings (rs = − 0.56, p = 0.011).

Conclusion

Speech with competitive noise and music are naturally present in everyday listening environments. Recent speech perception tests based on an adaptive paradigm and sentence materials in relation with music quality measures might be representative of everyday performance in CI users. The present data contribute to cross-language studies and suggest that improving music perception in CI users may yield everyday benefit in speech perception in noise and may hence enhance the quality of listening for CI users.
Tympanal bone fracture in forensic practice
Authors: Evain France , Lovblad Karl-Olof , Fracasso Tony
Published: October 2021

A tympanal bone fracture is an uncommon complication of head trauma and is mostly associated with a mandibular or petrous bone fracture. Upon reviewing the medicolegal literature, we could not find any publications on this topic. Tympanal bone fracture may lead to chronic complications (including external auditory canal stenosis and conductive hearing loss), with an important impact in both the medical and judiciary fields (e.g., chronic disabilities with loss of income). We decided to investigate the prevalence and mechanisms of tympanal bone fractures by means of a retrospective observational study on living victims who underwent head computed tomography after blunt head trauma and clinical forensic investigation at our center. We selected 159 cases of living victims with blunt head trauma (following an assault, traffic accident, or work accident) between January 2016 and December 2020. Re-examination of head imaging revealed 12 cases of tympanal bone fracture. Seven individuals showed cranial fractures involving the petrous bone (on the same side as the tympanal bone fracture). Three individuals had a temporomandibular fracture after a fall with chin impact. Only two victims exhibited an isolated tympanal bone fracture.
Surgery for chronic pancreatitis: the comparison of two high-volume centers reveals lack of a uniform operative management
Authors: Surci Niccolò , Bassi Claudio , Salvia Roberto , Marchegiani Giovanni , Casetti Luca , Deiro Giacomo , Bergmann Christina , Tamandl Dietmar , Schindl Martin , Mühlbacher Jakob , Sahora Klaus
Published: October 2021

Purpose

Many aspects of surgical therapy for chronic pancreatitis (CP), including the correct indication and timing, as well as the most appropriate operative techniques, are still a matter of debate in the surgical community and vary widely across different centers. The aim of the present study was to uncover and analyze these differences by comparing the experiences of two specialized surgical units in Italy and Austria.

Methods

All patients operated for CP between 2000 and 2018 at the two centers involved were included in this retrospective analysis. Data regarding the clinical history and the pre- and perioperative surgical course were analyzed and compared between the two institutions.

Results

Our analysis showed a progressive decrease in the annual rate of pancreatic surgical procedures performed for CP in Verona (no. = 91) over the last two decades (from 3% to less than 1%); by contrast, this percentage increased from 3 to 9% in Vienna (no. = 77) during the same time frame. Considerable differences were also detected with regard to the timing of surgery from the first diagnosis of CP — 4 years (IQR 5.5) in the Austrian series vs two (IQR 4.0) in the Italian series -, and of indications for surgery, with a 12% higher prevalence of groove pancreatitis among patients in the Verona cohort.

Conclusion

The comparison of the surgical attitude towards CP between two surgical centers proved that a consistent approach to this pathology still is lacking. The identification of common guidelines and labels of surgical eligibility is advisable in order to avoid interinstitutional treatment disparities.
Attitudes and opinions towards suicidality in professionals working with oncology patients: results from an online survey
Authors: Senf Bianca , Maiwurm Paula , Fettel Jens
Published: October 2021

Objective

To explore and describe attitudes and opinions towards suicidality in healthcare professionals (HCPs) working with oncological patients.

Methods

A 48-item online questionnaire was developed and distributed to HCPs working with cancer patients. Three hundred fifty-four answered questionnaires were analyzed.

Results

The majority of HCPs reported that they were able to understand why a cancer patient would commit suicide (87.8%) or would seek help from an assisted suicide organization (ASO; 83.9%). The understandable reasons were pain and physical impairments (51.4%), social isolation (19.8%), loss of control and autonomy (18.1%), terminal disease (17.2%), loss of meaning (15.3%), desperation (14.7%), and psychic distress (9.3%). Personal experiences with suicidality lead only 44.8% of HCPs to believe that thereby they would be better able to understand a patients’ wish for suicide. Religion was negatively associated with understanding of suicide and why a cancer patient would seek help from an ASO. Knowledge of suicidality was positively associated with why a cancer patient would seek help from an ASO.

Conclusions

There is still little knowledge in oncology about the relation of HCPs’ attitudes toward suicidality in their patients and how those attitudes influence their behavior, especially care and treatment of patients. More research on this topic is needed. It stands to reason that more education about suicidality in cancer patients seems likely to improve understanding and attitudes and thereby influence care for cancer patients.
Implicit assumptions and interpretation bias in youth with severe, chronic social phobia
Authors: Krömer Lisa , Jarczok Tomasz A. , Althen Heike , Mühlherr Andreas M. , Howland Vanessa , Jungmann Stefanie M. , Freitag Christine M.
Published: October 2021

Interpretation bias and dysfunctional social assumptions are proposed to play a pivotal role in the development and maintenance of social phobia (SP), especially in youth. In this study, we aimed to investigate disorder-specific implicit assumptions of rejection and implicit interpretation bias in youth with severe, chronic SP and healthy controls (CG). Twenty-seven youth with SP in inpatient/day-care treatment (M age = 15.6 years, 74% female) and 24 healthy controls (M age = 15.7 years, 54% female) were included. The Implicit Association Test (IAT) and the Affect Misattribution Procedure (AMP) were completed to assess implicit assumptions and interpretation bias related to the processing of social and affective stimuli. No group differences were observed for the IAT controlling for depressive symptoms in the analyses. However, group differences were found regarding interpretation bias (p = .017, η2p = .137). Correlations between implicit scores and explicit questionnaire results were medium to large in the SP group (r =|.28| to |.54|, pall ≤ .05), but lower in the control group (r =|.04| to |.46|, pall ≤ .05). Our results confirm the finding of an interpretation bias in youth SP, especially regarding the implicit processing of faces, whereas implicit dysfunctional social assumptions of being rejected do not seem to be specific for SP. Future research should investigate the causal relationship of assumptions/interpretation bias and SP.
The role of clay content and mineral surface area for soil organic carbon storage in an arable toposequence
Authors: Schweizer Steffen A. , Mueller Carsten W. , Höschen Carmen , Ivanov Pavel , Kögel-Knabner Ingrid
Published: October 2021

Correlations between organic carbon (OC) and fine mineral particles corroborate the important role of the abundance of soil minerals with reactive surfaces to bind and increase the persistence of organic matter (OM). The storage of OM broadly consists of particulate and mineral-associated forms. Correlative studies on the impact of fine mineral soil particles on OM storage mostly combined data from differing sites potentially confounded by other environmental factors. Here, we analyzed OM storage in a soil clay content gradient of 5–37% with similar farm management and mineral composition. Throughout the clay gradient, soils contained 14 mg OC g−1 on average in the bulk soil without showing any systematic increase. Density fractionation revealed that a greater proportion of OC was stored as occluded particulate OM in the high clay soils (18–37% clay). In low clay soils (5–18% clay), the fine mineral-associated fractions had up to two times higher OC contents than high clay soils. Specific surface area measurements revealed that more mineral-associated OM was related to higher OC loading. This suggests that there is a potentially thicker accrual of more OM at the same mineral surface area within fine fractions of the low clay soils. With increasing clay content, OM storage forms contained more particulate OC and mineral-associated OC with a lower surface loading. This implies that fine mineral-associated OC storage in the studied agricultural soils was driven by thicker accrual of OM and decoupled from clay content limitations.
Locust bean gum adsorption onto softwood kraft pulp fibres: isotherms, kinetics and paper strength
Authors: Chen Jingqian , Beatson Rodger P. , Trajano Heather L.
Published: October 2021

The adsorption of locust bean gum (LBG) onto Northern Bleached Softwood Kraft (NBSK) pulp improved paper tensile and burst strength and lowered refining energy by strengthening inter-fibre bonding. Adsorption kinetics and isotherms were investigated to develop a fundamental understanding of the adsorption mechanism. The adsorption rate followed pseudo-second-order kinetics and the activation energy was 99.34 kJ·mol−1, suggesting chemisorption. The adsorption rate constant increased rapidly with temperature from 25 to 45 °C (k = 1.93 to 24.03 g·mg−1·min−1), but the amount adsorbed at equilibrium decreased (qe = 1.91 to 0.48 mg·g−1 o.d. fibre). LBG adsorption to NBSK at 25 °C was consistent with the Langmuir adsorption model for LBG < 2.1 wt% of o.d. fibre, suggesting reversible, homogenous adsorption to a finite number of sites on the fibre surface. Refining to 3000 rev increased the heterogeneity of the NBSK pulp surface leading to multi-layer Freundlich adsorption with adsorption constant n = 5.00, and the equilibrium constant Kf = 2.57 mg·g−1·(mg·L−1)−1/n at 25 °C. Favorable adsorption conditions for negatively charged LBG were identified: 25 °C for 10 min, low dosage level (< 2 wt%), lightly refined (< 3000 rev) NBSK pulp at low fibre consistency (< 0.5 wt%), high agitation rate (> 150 r.p.m.), acidic or neutral conditions (pH 2–7) without salt addition.

Graphic abstract
Synergistic cytotoxicity of the CDK4 inhibitor Fascaplysin in combination with EGFR inhibitor Afatinib against Non-small Cell Lung Cancer
Authors: Plangger Adelina , Rath Barbara , Hochmair Maximilian , Funovics Martin , Neumayer Christoph , Zeillinger Robert , Hamilton Gerhard
Published: October 2021

In the absence of suitable molecular markers, non-small cell lung cancer (NSCLC) patients have to be treated with chemotherapy with poor results at advanced stages. Therefore, the activity of the anticancer marine drug fascaplysin was tested against primary NSCLC cell lines established from pleural effusions. Cytotoxicity of the drug or combinations were determined using MTT assays and changes in intracellular phosphorylation by Western blot arrays. Fascaplysin revealed high cytotoxicity against NSCLC cells and exhibit an activity pattern different of the standard drug cisplatin. Furthermore, fascaplysin synergizes with the EGFR tyrosine kinase inhibitor (TKI) afatinib to yield a twofold increased antitumor effect. Interaction with the Chk1/2 inhibitor AZD7762 confirm the differential effects of fascplysin and cisplatin. Protein phosphorylation assays showed hypophosphorylation of Akt1/2/3 and ERK1/2 as well as hyperphosphorylation of stress response mediators of H1299 NSCLC cells. In conclusion, fascaplysin shows high cytotoxicity against pleural primary NSCLC lines that could be further boosted when combined with the EGFR TKI afatinib.
The indolent lambdification of Java
Authors: Petrulio Fernando , Sawant Anand Ashok , Bacchelli Alberto
Published: October 2021

As Java 8 introduced functional interfaces and lambda expressions to the Java programming language, the JDK API was changed to introduce support for lambda expressions, thus allowing consumers to define lambda functions when using Java’s collections. While the JDK API allows for a functional paradigm, for API consumers to be able to completely embrace Java’s new functional features, third-party APIs must also support lambda expressions. To understand the current state of the Java ecosystem, we investigate (i) the extent to which third-party Java APIs have changed their interfaces, (ii) why or why not they introduce functional interface support and (iii) in the case the API has changed its interface how it does so. We also investigate the consumers’ perspective, particularly their ease in using lambda expressions in Java with APIs. We perform our investigation by manually analyzing the top 50 popular Java APIs, conducting in-person and email interviews with 23 API producers, and surveying 110 developers. We find that only a minority of the top 50 APIs support functional interfaces, the rest does not support them, predominantly in the interest of backward compatibility. Java 7 support is still greatly desirable due to enterprise projects not migrating to newer versions of Java. This suggests that the Java ecosystem is stagnant and that the introduction of new language features will not be enough to save it from the advent of new languages such as Kotlin (JVM based) and Rust (non-JVM based).
Collaborative process design for waste management: co-constructing strategies with stakeholders
Authors: Vasconcelos Lia T. , Silva Flávia Z. , Ferreira Filipa G. , Martinho Graça , Pires Ana , Ferreira José Carlos
Published: October 2021

Waste management is currently facing multiple challenges worldwide. The population growth in urban areas and related environmental concerns have increased the need to promote urban sustainability, namely where urban waste is a challenge. Thus, research on efficient waste management to reduce resources overload and to develop more sustainable production and consumption is key. Having this in mind, modern society is now seeking for an active voice and to be part of the decision-making processes that directly affect citizens. Citizens need to play an active role on decision-making to co-construct action guidelines, aiming a higher implementation success for an effective and inclusive management of urban waste. In this sense, UrbanWINS project was developed to promote co-construction and implementation of pilot actions involving citizens. In this paper the authors described the framework developed, and reflect critically on the results achieved.
Sediment underneath charophyte meadows is enriched in viable ephippia and enhances the benthic periphytic biofilm
Authors: Rodrigo María A. , Puche Eric , Segura Matilde , Arnal Adriana , Rojo Carmen
Published: October 2021

We contribute to the knowledge of charophyte meadows as key components of aquatic systems by analysing how they affect wetland sediments. We performed a factorial-design experiment with limnocorrals (outdoor mesocosms) in a Mediterranean protected wetland with presence or absence of charophytes [Chara vulgaris (CV) and Chara hispida (CH), planted from cultures or recruited in situ from germination of their fructifications]. The first 1 cm-surficial and 2 cm-bottom sediment layers were analysed for cladoceran ephippia, ostracods valves, benthic community of bacteria and periphytic biofilm, and charophyte fructifications. In the surficial sediment, the ephippia density was fourfold higher in the conditions with charophytes than in sites with no-charophytes and higher apparent viability was found. The surficial sediment periphyton biofilm was composed mainly of diatoms, with tenfold higher biomass underneath charophytes, and a much diverse community. The specific microhabitat generated by each charophyte species was reflected in the different abundances and relationships between the analysed components, firstly establishing a divergence with the sediment without meadows and, secondly, a distinction between the meadows of CH and CV that exhibit particular morphology-architecture, might exudate different metabolites and might have different allelopathic capacities over microalgae and microinvertebrates. Thus, the charophyte–sediment tandem is relevant for biodiversity and habitat conservation.
Educational leaders’ problem-solving for educational improvement: Belief validity testing in conversations
Authors: Sinnema Claire , Meyer Frauke , Le Fevre Deidre , Chalmers Hamish , Robinson Viviane
Published: October 2021

Educational leaders’ effectiveness in solving problems is vital to school and system-level efforts to address macrosystem problems of educational inequity and social injustice. Leaders’ problem-solving conversation attempts are typically influenced by three types of beliefs—beliefs about the nature of the problem, about what causes it, and about how to solve it. Effective problem solving demands testing the validity of these beliefs—the focus of our investigation. We analyzed 43 conversations between leaders and staff about equity related problems including teaching effectiveness. We first determined the types of beliefs held and the validity testing behaviors employed drawing on fine-grained coding frameworks. The quantification of these allowed us to use cross tabs and chi-square tests of independence to explore the relationship between leaders’ use of validity testing behaviors (those identified as more routine or more robust, and those relating to both advocacy and inquiry) and belief type. Leaders tended to avoid discussion of problem causes, advocate more than inquire, bypass disagreements, and rarely explore logic between solutions and problem causes. There was a significant relationship between belief type and the likelihood that leaders will test the validity of those beliefs—beliefs about problem causes were the least likely to be tested. The patterns found here are likely to impact whether micro and mesosystem problems, and ultimately exo and macrosystem problems, are solved. Capability building in belief validity testing is vital for leadership professional learning to ensure curriculum, social justice and equity policy aspirations are realized in practice.
Enhanced detection of atrial tachyarrhythmias with pacing devices by using more accurate atrial sensing
Authors: Pakarinen Sami , Lehto Mika , Ruiter Jaap , de Voogt Willem G.
Published: October 2021

Purpose

Cardiac pacing devices can detect and monitor atrial tachyarrhythmias (ATA) which increase the risk of thromboembolic complications. The aim of this study was to compare (1) two different atrial leads and (2) standard and optimized settings to detect ATA and reject far-field R-wave signal (FFRW).

Methods

This was a prospective, randomized multi-center trial comparing St. Jude Medical OptiSense lead (tip-to-ring spacing 1.1 mm) and Tendril lead (tip-to-ring spacing 10.0 mm), having programmed atrial sensitivity at 0.2 mV and post-ventricular atrial blanking at 60 ms. We measured intra-atrial amplitudes of FFRW, intrinsic atrial signals, the amount of FFRW oversensing, and other inappropriate mode switching.

Results

One hundred and ten patients were enrolled. The mean amplitude of sensed and paced FFRW bipolar signal was 0.13 mV vs. 0.21 mV (p < 0.001) and 0.13 mV vs. 0.26 mV (p < 0.001) with OptiSense and Tendril lead, respectively. The mean amplitude of the atrial bipolar signal was 2.84 mV with OptiSense and 3.48 mV with Tendril lead, p = 0.014. With the optimized settings with OptiSense lead, one patient out of 20 (5%) had FFRW oversensing, none had undersensing of ATAs due to 2:1-blanking of atrial depolarizations, and the concordance of the ATAs by Holter and pacemaker memory was high (Spearman’s rank correlation coefficient = 0.90). In the Tendril group, 12 out of 25 patients (48%) had oversensing and 4 had atrial undersensing (p < 0.001).

Conclusions

The technique with an atrial lead with short tip-to-ring spacing combined with optimized pacemaker programming resulted in reliable and accurate atrial arrhythmia detection.

Trial registration

ClinicalTrials.gov number NCT01074749.
Bowel Histology of CVID Patients Reveals Distinct Patterns of Mucosal Inflammation
Authors: van Schewick Cornelia M. , Lowe David M. , Burns Siobhan O. , Workman Sarita , Symes Andrew , Guzman David , Moreira Fernando , Watkins Jennifer , Clark Ian , Grimbacher Bodo
Published: October 2021

Diarrhea is the commonest gastrointestinal symptom in patients with common variable immunodeficiency (CVID). Different pathologies in patients’ bowel biopsies have been described and links with infections have been demonstrated. The aim of this study was to analyze the bowel histology of CVID patients in the Royal-Free-Hospital (RFH) London CVID cohort. Ninety-five bowel histology samples from 44 adult CVID patients were reviewed and grouped by histological patterns. Reasons for endoscopy and possible causative infections were recorded. Lymphocyte phenotyping results were compared between patients with different histological features. There was no distinctive feature that occurred in most diarrhea patients. Out of 44 patients (95 biopsies), 38 lacked plasma cells. In 14 of 21 patients with nodular lymphoid hyperplasia (NLH), this was the only visible pathology. In two patients, an infection with Giardia lamblia was associated with NLH. An IBD-like picture was seen in two patients. A coeliac-like picture was found in six patients, four of these had norovirus. NLH as well as inflammation often occurred as single features. There was no difference in blood lymphocyte phenotyping results comparing groups of histological features. We suggest that bowel histology in CVID patients with abdominal symptoms falls into three major histological patterns: (i) a coeliac-like histology, (ii) IBD-like changes, and (iii) NLH. Most patients, but remarkably not all, lacked plasma cells. CVID patients with diarrhea may have an altered bowel histology due to poorly understood and likely diverse immune-mediated mechanisms, occasionally driven by infections.
Column generation for minimizing total completion time in a parallel-batching environment
Authors: Alfieri A. , Druetto A. , Grosso A. , Salassa F.
Published: October 2021

This paper deals with the $$1|{p-\text {batch}, s_j\le b}|\sum C_j$$

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scheduling problem, where jobs are scheduled in batches on a single machine in order to minimize the total completion time. A size is given for each job, such that the total size of each batch cannot exceed a fixed capacity b. A graph-based model is proposed for computing a very effective lower bound based on linear programming; the model, with an exponential number of variables, is solved by column generation and embedded into both a heuristic price and branch algorithm and an exact branch and price algorithm. The same model is able to handle parallel-machine problems like $$Pm|{p-\text {batch}, s_j\le b}|\sum C_j$$

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very efficiently. Computational results show that the new lower bound strongly dominates the bounds currently available in the literature, and the proposed heuristic algorithm is able to achieve high-quality solutions on large problems in a reasonable computation time. For the single-machine case, the exact branch and price algorithm is able to solve all the tested instances with 30 jobs and a good amount of 40-job examples.
Dimensions of Fractional Brownian Images
Authors: Burrell Stuart A.
Published: October 2021

This paper concerns the intermediate dimensions, a spectrum of dimensions that interpolate between the Hausdorff and box dimensions. Potential-theoretic methods are used to produce dimension bounds for images of sets under Hölder maps and certain stochastic processes. We apply this to compute the almost-sure value of the dimension of Borel sets under index-$$\alpha $$
α
fractional Brownian motion in terms of dimension profiles defined using capacities. As a corollary, this establishes continuity of the profiles for Borel sets and allows us to obtain an explicit condition showing how the Hausdorff dimension of a set may influence the typical box dimension of Hölder images such as projections. The methods used propose a general strategy for related problems; dimensional information about a set may be learned from analysing particular fractional Brownian images of that set. To conclude, we obtain bounds on the Hausdorff dimension of exceptional sets, with respect to intermediate dimensions, in the setting of projections.
Treatment trials in Niemann-Pick type C disease
Authors: Sitarska Dominika , Tylki-Szymańska Anna , Ługowska Agnieszka
Published: October 2021

Niemann-Pick type C (NPC) disease is a genetically determined neurodegenerative metabolic disease. It belongs to the lysosomal storage diseases and its main cause is impaired cholesterol transport in late endosomes or lysosomes. It is an autosomal recessive inherited disease that results from mutations in the NPC1 or NPC2 genes. The treatment efforts are focused on the slowing its progression. The only registered drug, devoted for NPC patients is Miglustat. Effective treatment is still under development. NPC disease mainly affects the nervous system, and the crossing of the blood–brain barrier by medicines is still a challenge, therefore the combination therapies of several compounds are increasingly being worked on. The aim of this paper is to present the possibilities in treatment of Niemann-Pick type C disease. The discussed research results relate to animal studies.
Transient gas pipeline flow: analytical examples, numerical simulation and a comparison to the quasi-static approach
Authors: Gugat Martin , Krug Richard , Martin Alexander
Published: October 2021

The operation of gas pipeline flow with high pressure and small Mach numbers allows to model the flow by a semilinear hyperbolic system of partial differential equations. In this paper we present a number of transient and stationary analytical solutions of this model. They are used to discuss and clarify why a PDE model is necessary to handle certain dynamic situations in the operation of gas transportation networks. We show that adequate numerical discretizations can capture the dynamical behavior sufficiently accurate. We also present examples that show that in certain cases an optimization approach that is based on multi-period optimization of steady states does not lead to approximations that converge to the optimal state.
Process-Dependent Solute Transport in Porous Media
Authors: Erfani Hamidreza , Karadimitriou Nikolaos , Nissan Alon , Walczak Monika S. , An Senyou , Berkowitz Brian , Niasar Vahid
Published: October 2021

Solute transport under single-phase flow conditions in porous micromodels was studied using high-resolution optical imaging. Experiments examined loading (injection of ink-water solution into a clear water-filled micromodel) and unloading (injection of clear water into an ink-water filled micromodel). Statistically homogeneous and fine-coarse porous micromodels patterns were used. It is shown that the transport time scale during unloading is larger than that under loading, even in a micromodel with a homogeneous structure, so that larger values of the dispersion coefficient were obtained for transport during unloading. The difference between the dispersion values for unloading and loading cases decreased with an increase in the flow rate. This implies that diffusion is the key factor controlling the degree of difference between loading and unloading transport time scales, in the cases considered here. Moreover, the patterned heterogeneity micromodel, containing distinct sections of fine and coarse porous media, increased the difference between the transport time scales during loading and unloading processes. These results raise the question of whether this discrepancy in transport time scales for the same hydrodynamic conditions is observable at larger length and time scales.
Benthic macroinvertebrates as reference indicators for monitoring of anthropogenic isotope 137Cs contamination in the marine environment
Authors: Saniewski Michał , Zalewska Tamara , Kraśniewski Wojciech
Published: October 2021

Due to the limited studies dealing with isotopes levels in benthic macroinvertebrates other than Mytilus sp. in period 2011–2018, macroinvertebrates and seawater samples from the southern Baltic Sea were collected. Activity of 137Cs was measured in most common benthic macroinvertebrates inhabiting southern Baltic Sea in the context of choosing the best bioindicators. Taking into account the narrow range of CR coefficients, statistically significant correlation between 137Cs activities in seawater and species, a high frequency of occurrence and thus high biomass and the common occurrence, only Limecola balthica could potentially serve as indicators for shallow and deep-sea areas accordingly. Widely spread Mytillus trossulus was characterised by large size variability in samples and shows no significant correlation between activities in species and seawater and appears not so common as L. balthica.
Preventing Violence through Participation in Community Building in Youth
Authors: Cheung Chau-kiu
Published: October 2021

Youth’s participation in community service is a proposed but uncharted way to prevent their violent perpetration. To clarify the preventive function, this study analyzes two-wave panel survey data on 1,710 Chinese youths in Hong Kong according to empowerment theory. Specifically, the theory posits that empowerment functions when it targets youth plagued by powerlessness. Two hypothesized conditions of relative powerlessness are being female and living in poor housing. Results support the hypotheses when participation in community service appeared to prevent violent perpetration, and the prevention was greater under the two powerless conditions. These results importantly emerged with the control for prior violent perpetration and adjustment for selectivity into the participation. The results thus imply the value of inviting youth to participate in community service to prevent their violent perpetration. The invitation can target youth who are female or residing in poor housing.
A New Method for a Shorter and Valid Assessment of Olfactory Threshold in Repeated Measurement Designs Based on the Sniffin’ Sticks Test
Authors: Beutler Sarah , Ladner Liliana R. , Hummel Thomas , Croy Ilona
Published: October 2021

Introduction

The Sniffin’ Sticks threshold test is widely used to assess olfactory threshold due to its high reliability and validity. Nevertheless, this test procedure is quite long with an average duration of 10 to 20 min. In study designs that require multiple olfactory threshold tests on short intervals, this could exhaust participants. To counteract this limitation, we developed the informed-four-reversal (INFOUR) short version of the Sniffin’ Sticks threshold test for repeated measurement designs and piloted it in a sample of normosmic participants.

Methods

Forty-two participants performed the original Sniffin’ Sticks version before being assigned either to the control group that repeated the original version or to the test group that conducted the INFOUR short version.

Results

The correlation between the original version at T1 and INFOUR at T2 was r = .75 and did not differ significantly from the retest reliability of the original version. Compared to the original version, the INFOUR took 42% less time to perform.

Conclusion

The INFOUR leads to a significant time saving, while maintaining good validity.

Implications

Therefore, this approach has the potential to be a useful tool for study protocols with repeated olfactory threshold measurements. In particular, when research protocols are time intensive or testing needs to the shortened, because the interventional effects are short or subtle.
Characterization of hydrothermal alteration along geothermal wells using unsupervised machine-learning analysis of X-ray powder diffraction data
Authors: Ishitsuka Kazuya , Ojima Hiroki , Mogi Toru , Kajiwara Tatsuya , Sugimoto Takeshi , Asanuma Hiroshi
Published: October 2021

Zonal distribution of hydrothermal alteration in and around geothermal fields is important for understanding the hydrothermal environment. In this study, we assessed the performance of three unsupervised classification algorithms—K-mean clustering, the Gaussian mixture model, and agglomerative clustering—in automated categorization of alteration minerals along wells. As quantitative data for classification, we focused on the quartz indices of alteration minerals obtained from rock cuttings, which were calculated from X-ray powder diffraction measurements. The classification algorithms were first examined by applying synthetic data and then applied to data on rock cuttings obtained from two wells in the Hachimantai geothermal field in Japan. Of the three algorithms, our results showed that the Gaussian mixture model provides classes that are reliable and relatively easy to interpret. Furthermore, an integrated interpretation of different classification results provided more detailed features buried within the quartz indices. Application to the Hachimantai geothermal field data showed that lithological boundaries underpin the data and revealed the lateral connection between wells. The method’s performance is underscored by its ability to interpret multi-component data related to quartz indices.
Recombinant Avian β-Defensin Produced by Food-Grade Lactococcus as a Novel and Potent Immunological Enhancer Adjuvant for Avian Vaccine
Authors: Wang Tingting , Wang Zhihao , Mi Jielan , Wang Wenqian , Li Kai , Qi Xiaole , Gao Yulong , Gao Li , Liu Changjun , Zhang Yanping , Pan Qing , Wang Xiaomei , Cui Hongyu
Published: October 2021

In this study, we expressed rAvBD1-2–6-13 protein throughLactococcus lactisNZ3900, and the effects of the recombinantL. lactisNZ3900 as an immune enhancer and immune adjuvant were verified using in vivo and in vitro tests. In vitro tests revealed that recombinantL. lactisNZ3900 significantly activated the NF-κB signaling pathway and IRF signaling pathway in J774-Dual™ report cells and significantly increased the transcript levels of IL-10, IL-12p70, CD80, and CD86 in chicken PBMCs and chicken HD11 cells. In vivo experiments revealed that the immunized group supplemented with recombinantL. lactisNZ3900 as an adjuvant had significantly higher serum antibody titers and higher proliferative activity of PBMCs in the blood of the chickens immunized with NDV live and inactivated vaccines. Our study shows that the recombinantL. lactisNZ3900 has strong immunomodulatory activity both in vivo and in vitro and is a potential immune enhancer. Our work lays the foundation for the research and development of new animal immune enhancers for application in the poultry industry.
The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response
Authors: Jarosz Łukasz , Ciszewski Artur , Marek Agnieszka , Grądzki Zbigniew , Kaczmarek Beata , Rysiak Anna
Published: October 2021

The aim of the study was to determine the effect of EM Bokashi® on selected parameters of the specific and nonspecific immune response of sows by in colostrum and milk samples. The percentage of cells with expression of CD19+, CD5+CD19+, CD21+, SWC3a (macrophage/monocyte), and CD11b+molecules on the monocytes and granulocytes as well as the concentrations of lysozyme and acute phase proteins — serum amyloid-A (SAA) and haptoglobin (Hp) were evaluated. The study was carried out on a commercial pig farm, including 150 sows (Polish Large White × Polish Landrace) at the age of 2–4 years. Sixty female sows were divided into two groups: I — control and II — experimental. For the experimental group, a probiotic in the form of the preparation EM Bokashi® in the amount of 10 kg/tonne of feed was added to the basal feed from mating to weaning. The material for the study consisted of colostrum and milk. The samples were collected from all sows at 0, 24, 48, 72, 96, 120, 144, and 168 h after parturition. The study showed that exposure of the pregnant sow to the probiotic microbes contained in EM Bokashi® significantly affects the immunological quality of the colostrum and milk and caused an increase in the percentage of the subpopulations of B cells with CD19+, CD21+, and CD5+CD19+expression in the colostrum and milk, which demonstrates an increase in the protective potential of colostrum and indicates stimulation of humoral immune mechanisms that protect the sow and the piglets against infections.
Molecular markers associated with the outcome of tamoxifen treatment in estrogen receptor-positive breast cancer patients: scoping review and in silico analysis
Authors: Dal Berto Maiquidieli , dos Santos Giovana Tavares , dos Santos Aniúsca Vieira , Silva Andrew Oliveira , Vargas José Eduardo , Alves Rafael José Vargas , Barbisan Fernanda , da Cruz Ivana Beatrice Mânica , Bica Claudia Giuliano
Published: October 2021

Tamoxifen (TMX) is used as adjuvant therapy for estrogen receptor-positive (ER+) breast cancer cases due to its affinity and inhibitory effects. However, about 30% of cases show drug resistance, resulting in recurrence and metastasis, the leading causes of death. A literature review can help to elucidate the main cellular processes involved in TMX resistance. A scoping review was performed to find clinical studies investigating the association of expression of molecular markers profiles with long-term outcomes in ER+ patients treated with TMX. In silico analysis was performed to assess the interrelationship among the selected markers, evaluating the joint involvement with the biological processes. Forty-five studies were selected according to the inclusion and exclusion criteria. After clustering and gene ontology analysis, 23 molecular markers were significantly associated, forming three clusters of strong correlation with cell cycle regulation, signal transduction of proliferative stimuli, and hormone response involved in morphogenesis and differentiation of mammary gland. Also, it was found that overexpression of markers in selected clusters is a significant indicator of poor overall survival. The proposed review offered a better understanding of independent data from the literature, revealing an integrative network of markers involved in cellular processes that could modulate the response of TMX. Analysis of these mechanisms and their molecular components could improve the effectiveness of TMX.
It is really time to retire laparoscopic gastric banding? Positive outcomes after long-term follow-up: the management is the key
Authors: Lucido Francesco Saverio , Scognamiglio Giuseppe , Nesta Giusiana , del Genio Gianmattia , Cristiano Stefano , Pizza Francesco , Tolone Salvatore , Brusciano Luigi , Parisi Simona , Pagnotta Stefano , Gambardella Claudio
Published: October 2021

After the initial widespread diffusion, laparoscopic adjustable gastric banding (LAGB) has been progressively abandoned and laparoscopic sleeve gastrectomy (LSG) has become the worldwide most adopted procedure. Nevertheless, recent reports raised concerns about the long-term weight regain after different bariatric techniques. Considering the large LAGB series recorded in our multicentric bariatric database, we analysed the anthropometric and surgical outcomes of obese patients underwent LAGB at a long-term follow-up, focusing on LAGB management. Between January 2008 to January 2018, demographics, anthropometric and post-operative data of obese patients undergone LAGB were retrospectively evaluated. To compare the postoperative outcomes, the cohort was divided in two groups according to the quantity of band filling (QBF): low band filling group (Group 1) with at most 3 ml of QBF, and patients in the high band filling group (Group 2) with at least 4 ml. 699 obese patients were considered in the analysis (351 in Group 1 and 348 in Group 2). Patients in Group 1 resulted significantly associated (p < 0.05) to higher % EWL and quality of life score (BAROS Score), 49.1 ± 11.3 vs 38.2 ± 14.2 and 5.9 ± 1.8 vs 3.8 ± 2.5, respectively. Moreover, patients with lower band filling (Group 1) complained less episodes of vomiting, epigastric pain and post-prandial reflux and significantly decreased slippage and migration rate (p < 0.001 for all parameters). LAGB is a safe and reversible procedure, whose efficacy is primarily related to correct postoperative handling. Low band filling and strict follow-up seem the success’ key of this technique, which deserves full consideration among bariatric procedures.