bluuebunny/crossref_metadata_2025 huggingface dataset:
Acute Effect of a Single Functional Neurology Intervention on Muscular Trigger Point
Authors: Rey-Mota Jorge , Escribano-Colmena Guillermo , Dalamitros Athanasios A. , Yáñez-Sepúlveda Rodrigo , Álvarez David Martín-Caro , Jimenez Eduardo Navarro , Clemente-Suárez Vicente Javier
Published: February 2025

Background: Myofascial trigger points (MTrPs) are hyperirritable spots in skeletal muscle associated with pain and dysfunction, often impacting individuals’ quality of life. Various interventions, such as dry needling and manual therapy, have shown limited effects in addressing these conditions. This study aimed to assess the effectiveness of a functional neurology intervention in reducing pain and improving muscle function in patients with MTrPs in the upper trapezius muscle. We hypothesized that a single session of functional neurology intervention would significantly increase the pressure pain threshold (PPT) and improve peripheral vascular response in individuals with myofascial trigger points compared to a control group. Methods: A randomized controlled trial (RCT) was conducted with 63 participants randomly assigned to an experimental (receiving functional neurology treatment) or control group. Pre- and post-treatment assessments were conducted, and both intra- and inter-group comparisons were performed using algometry to measure the PPT and infrared thermography to analyze peripheral vascular response. Data were analyzed using dependent and independent t-tests with statistical significance set at p < 0.05. Results: The experimental group demonstrated a significant 46.4% increase in PPT, while the control group showed negligible changes. Thermographic analysis indicated improved peripheral blood flow in the experimental group, reflected by increased skin temperatures and reduced thermal anomalies. No significant differences were observed between the groups at baseline. Conclusions: A single session of functional neurology intervention significantly reduced pain and improved muscle function in patients with MTrPs. These findings suggest that functional neurology offers a promising non-invasive alternative to traditional treatments, with potential implications for more rapid and sustained therapeutic outcomes.
Methodology to Determine the Stress Distribution Based on Fatigue Data with Bilinear Behavior and Its P–S–N Field and Testing Plan
Authors: Monclova-Quintana Osvaldo , Piña-Monarrez Manuel R. , Hernández-Ramos María M. , Ortiz-Yáñez Jesús F.
Published: February 2025

In this paper, based on the Weibull Inverse Power Law, we present a methodology to determine the following: (1) the failure percentiles, referred to as the P–S–N field, of an S–N curve for a 42CrMo4 steel material exhibiting bilinear (s1 and s2) behavior (e.g., a competence failure mode); (2) the Weibull family that characterizes the entire bilinear behavior; and (3) the zero-vibration test plan that meets the required vibration reliability index of Rt=0.97 with a reliability confidence level of CL=0.75. From the application, based on the formulated normal–Weibull relationship, we determine the failure percentiles for the normal (one, two, and three) sigma levels, as well as those failure percentiles corresponding to the capability (Cp) and ability (Cpk) indices. Finally, we present the formulation to determine the Rt index and the CL level associated with each normal percentile, along with their numerical values.
The Hydrodynamic Simulation of Karst Water Under Deep Coal Mining and Fault Conditions: A Case Study of the Zhuxianzhuang Mine in Northern Anhui
Authors: Xu Zhenghao , Liu Qimeng , Chen Kai , Chai Huichan , Ju Qiding
Published: February 2025

As shallow coal resources in China become increasingly depleted, deep coal mining in complex geological areas has become an inevitable trend. However, the technical challenges associated with deep mining are becoming more significant, particularly the issues related to mine water hazards. This study utilized hydrogeological data from the III3 Mining Area in the Zhuxianzhuang Coal Mine, Anhui Province, and employed GMS (Groundwater Modeling System) software to construct a numerical karst water flow model under deep mining conditions. By simulating variations in the flow field, the study verified the drainage potential of the limestone water at the base of Seam 10 and assessed the water conductivity and connectivity of the F22 fault. The following conclusions were obtained: The simulation effectively captured the formation process of the karst water drawdown cone in the study area. The observed water level variations in different monitoring wells aligned well with the engineering reality after validation. The limestone water at the base of Seam 10 in the III3 Mining Area exhibited good transmissivity, weak recharge, and high drainage potential. Although the F22 fault is a normal fault with a maximum displacement of 550 m, offsetting formations from Seam 3 to the Ordovician limestone, its connectivity and water conductivity are poor, exhibiting significant water-blocking properties. The specific capacity (q) ranges from 1.40 × 10−4 to 3.26 × 10−3 m3/(s·m), and the hydraulic conductivity (K) ranges from 2.10 × 10−5 to 6.80 × 10−5. Under deep coal mining conditions, the extraction of coal disturbs the underlying limestone, generally resulting in an increase in its permeability coefficient compared to pre-mining conditions. The permeability coefficient (K) from the measured data before mining impact ranged from 0.000067 to 0.0022, while the simulated values after mining impact ranged from 0.0021 to 0.09. Additionally, mining activities affect the hydraulic head, flow rate, and flow paths of the karst water; the floor karst water is easily drainable, effectively reducing water pressure and the inrush coefficient, thus lowering water hazard risks. Although the mining area is affected by the large F22 fault, its water-resisting properties under sufficient drainage conditions prevent direct connectivity between the coal seam and the aquifer, avoiding water hazards. As global coal resources continue to be exploited, deep mining will inevitably become a common trend in coal extraction worldwide. This study develops a hydrogeological model tailored to deep mining under fault conditions, offering a solid theoretical foundation and practical reference for the prevention and management of mine water hazards on a global scale. This advancement contributes to the development of sustainable mining practices across the global industry.
A Review on Achieving Sustainability in the Petroleum Industry Through the Integration of Lean and Green
Authors: Dibia Felister , Dibia Chinedu , Dhakal Hom Nath , Okpako Oghenovo , Radulovic Jovana , Isike Augustine
Published: February 2025

The petroleum industry has been a key driver in the development of the world economy yet continues to attract increasing criticism due to its negative environmental impact via greenhouse gas emissions, resource inefficiency, and waste. Integrating lean and green practices is fast becoming a transformative approach to tackling these issues as it integrates process optimization with sustainability principles. This review paper examined the existing literature on lean and green integration, highlighting its benefits, models, critical success factors, and a roadmap for its implementation. Also, it identified sustainability challenges and offered strategic solutions. The findings showed that integrating lean and green offers potential for both process optimization and waste and carbon footprint reduction, particularly for the petroleum industry in Sub-Saharan Africa (SSA). This can be achieved by using appropriate tools and models. Sustainable value stream mapping (Sus-VSM) is a strategic tool that highlights the importance of sustainability metrics. These sustainability metrics address the implementation challenges of the convectional value stream mapping tool (VSM). This paper is one of the few initiatives to promote lean–green integration within the petroleum industry.
Dietary Methionine Hydroxy Analog Regulates Hepatic Lipid Metabolism via SIRT1/AMPK Signaling Pathways in Largemouth Bass Micropterus salmodies
Authors: Zhao Ju , Yang Zhongjie , Liu Haifeng , Yang Chao , Chen Yujun , Cao Quanquan , Jiang Jun
Published: February 2025

This experiment was arranged to explore the impacts of dietary MHA on liver lipid metabolism in largemouth bass. A total of 480 fish (14.49 ± 0.13 g) were randomly allocated into four groups, each with three replicates. They were then given four different diets containing graded levels of MHA (0.0, 3.0, 6.0, and 9.0 g/kg) for 84 days. The results showed that dietary MHA increased hepatic lipid vacuoles and lipid content (p < 0.05). Dietary supplementation with MHA 9.0 g/kg diets increased the activities of acetyl-coA carboxylase (ACC), fatty acid synthase (FAS), and stearoyl-coA desaturase 1 (SCD-1). Dietary MHA up-regulated the mRNA expressions of liver lipid synthesis (ACC, FAS, SCD-1 and SREBP-1c) (p < 0.05). Furthermore, compared with the 0.0 g/kg diet group, the group supplemented with 9.0 g/kg MHA in the diet exhibited a significant decrease in the activities of liver lipid-oxidation-related enzymes (acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and stearoyl-CoA desaturase 1 (SCD-1), as well as HSL and CPT1) and the gene expressions of ATGL, HSLa, HSLb, CPT1a, and PPARα (p < 0.05). Additionally, the mRNA expressions and protein levels of SIRT1 and AMPK in the 9.0 g/kg MHA-supplemented group were significantly lower than those in the 0.0 g/kg diet group (p < 0.05). Overall, the present results suggested that dietary MHA could increase lipid accumulation through regulating SIRT1/AMPK signaling pathways in the livers of largemouth bass.
Pharmacological Interventions for Negative Symptoms in Schizophrenia: A Systematic Review of Randomised Control Trials
Authors: Moccia Lorenzo , Bardi Francesca , Anesini Maria Benedetta , Barbonetti Sara , Kotzalidis Georgios D. , Rossi Sara , Caso Romina , Grisoni Flavia , Mandracchia Giuseppe , Margoni Stella , Callovini Tommaso , Janiri Delfina , Mazza Marianna , Simonetti Alessio , Montanari Silvia , Autullo Gianna , Camardese Giovanni , Pepe Maria , Di Nicola Marco , Di Giorgio Vassilij , Conti Fabio , Sani Gabriele ,   on behalf of the Gemelli RePsy Study Group
Published: February 2025

Background/Objectives: While positive symptoms of schizophrenia are often satisfactorily controlled, negative symptoms are difficult to treat, persisting despite treatment. Different strategies have been devised to deal with this problem. We aimed to review drug treatment for negative symptoms of schizophrenia in controlled trials of marketed drugs. Methods: We searched the PubMed database and the resulting records’ reference lists to identify eligible trials using schizophrenia[ti] AND “negative symptom*”[ti] as a search strategy. We determined eligibility through Delphi rounds among all authors. Results: On 11 February 2025, we identified 1485 records on PubMed and 3 more from reference lists. Eligible were 95 records. Most studies were double-blind, randomized controlled trials, carried-out in add-on in patients stabilized with antipsychotics. Other antipsychotics were the most frequent comparators, followed by antidepressants, and recently, antioxidants are gaining importance in trials. Many trials, especially those conducted in the Western world, found no significant effects compared to placebo, while most Iranian studies were positive, although not with a strong effect size. Conclusions: Current research has contributed little to progress in the treatment of the negative symptoms of schizophrenia. The reason might reside in the absence of knowledge of the mechanisms whereby these symptoms are generated, which prevents us from designing possibly effective treatment strategies, and/or to the chronicity of negative symptoms, as they are the first to be established even when they do not become fully apparent.
Spatiotemporal Evolution and Influencing Factors of Residential Prices in Zhengzhou
Authors: Wang Yafei , Cui Tian , Zhong Wenyu , Liu Wenkai , Hu Qingfeng , Zhang Bing
Published: February 2025

The dynamic fluctuations in the real estate market significantly impact the development of the national economy. Investigating the spatiotemporal characteristics of housing prices can assist the government in formulating rational regulatory policies. Taking Zhengzhou City as the research subject, this study analyzed the spatiotemporal characteristics of housing prices based on housing price data and POI (Point of Interest) data from January 2022 to March 2024, utilizing a spatial scale of 500 m × 500 m grids. A hedonic price model and a geographically weighted regression (GWR) model were constructed to examine the mechanisms of 12 influencing factors on housing prices. The results indicate that housing prices in the eastern part of Zhengzhou are higher than those in the west, with an overall declining trend observed in Zhengzhou’s housing prices. Among the influencing factors, the age of the house exerts the greatest impact on housing prices, while finance has the least influence. The GWR model demonstrates superior fitting performance compared to the hedonic price model. The mechanisms of the influencing factors exhibit spatial heterogeneity. This study provides valuable insights for relevant government departments in Zhengzhou City, contributing to the optimization of urban planning and the regulation of the real estate market.
Assessment of Phoenix Sepsis Score, pSOFA, PELOD-2, and PRISM III in Pediatric Intensive Care
Authors: Hadzhieva-Hristova Adriana , Krumova Darina , Stoeva Temenuga , Georgieva Ralitza , Iotova Violeta
Published: February 2025

Background/Objectives: Early identification of pediatric sepsis complications in intensive care is challenging and requires improved diagnostic tools. This study aimed to compare the Phoenix Sepsis Score (PSS), pSOFA, PELOD-2, and PRISM III in assessing clinical complexity in children with septic and critical conditions in the PICU and to identify the most suitable scale for this patient cohort. Methods: Data were collected prospectively from 53 children between June 2022 and January 2024. Patients were categorized into septic (n = 42) and non-infectious SIRS (n = 11) and further classified by outcome—with/without complications (n = 23/30). The predictive accuracy of the scoring systems was evaluated by discrimination and calibration and by recalibration for the PSS for improved performance. Results: Respiratory (18.8%) and neurological complications (9.4%) were the most common adverse events. Clinical deterioration was observed in 43.4% of cases, including one fatality. Patients with complications stayed longer in the PICU (14 ± 10 days). In the patients with complications, all scoring systems had higher median values. Only PSS showed a significant difference (p = 0.0023). PSS demonstrated the highest overall predictive accuracy (76.2%) outperforming PRISM III (62.3%) and PELOD-2 (58.5%). The pSOFA scale showed high accuracy (88.0%) in identifying patients without complications. The strongest association was between chronic disease (hazard ratio Exp(B) = 1.718) and deteriorations, while mechanical ventilation suggested a reduced risk of complications (Exp(B) = 0.509). Conclusions: PSS showed superior predictive accuracy (76.2%) for deteriorations in pediatric patients with suspected infection and proved adaptable for further validation in larger populations.
Enhancing Strength and Ductility in the Nugget Zone of Friction Stir Welded X80 Pipeline Steel via Applying Cooling Medium
Authors: Duan Ruihai , Xie Guangming , Qi Xiaonan , Wang Zhaojie , Chen Shujin , Dong Ying
Published: February 2025

Fusion welding easily causes microstructural coarsening and tempering softening in the heat-affected zone (HAZ) of high-strength pipeline steel joints, which considerably deteriorates the strength and toughness. Here, X80 pipeline steel was subjected to friction stir welding (FSW), and external cooling was used to tailor the microstructure to optimize the strength–ductility combination of the nugget zone (NZ). Coarse granular bainite (GB) appeared at air cooling, whereas a fine ferrite/martensite microstructure was achieved at solid CO2 cooling. The highest ratio of high-angle boundaries was obtained at solid CO2 cooling because the variants were evenly distributed within the four close-packed (CP) groups. The low yield strength (YS) of 595 MPa was obtained in the NZ under air cooling, whereas a high YS of 755 MPa was achieved in the NZ under solid CO2 cooling due to dislocation strengthening and fine-grain strengthening. Furthermore, an ultra-high tensile strength of 910 MPa and utilizable elongation of 15% were obtained in the NZ under solid CO2 cooling, which was attributed to the fine effective grains and ferrite/martensite microstructure facilitating a ductile fracture.
A Framework for Communication–Compute–Control Co-Design in Cyber–Physical Systems
Authors: Grosjean Leefke , Sachs Joachim , Ansari Junaid , Reider Norbert , Hernandez Herranz Aitor , Holmberg Christer
Published: February 2025

Cyber–physical systems are created at the intersection of physical processes, networking, and computation. For applications developed to implement cyber–physical interactions, in the face of limited resources, an optimization of efficiency needs to be handled across all entities—communication, computing, and control. This gives rise to the emerging area of “co-design” that addresses the challenge of designing applications or systems at the intersection of control, communication, and compute when these domains can and should no longer be considered to be fully independent. In this article a co-design framework is presented that provides a structured way of addressing the co-design problem. Different co-design degrees are specified that group application design approaches according to their needs on criticality/dependability and relate these to the knowledge, insights, and required interactions with the communication and computation infrastructure. The applicability of the framework is illustrated for the example of autonomous mobile robots, showing how different co-design degrees exploit the relationships and permit the identification of technical solutions that achieve improved resource efficiency, increased robustness, and improved performance compared to traditional application design approaches. The framework is of relevance both for concrete near-term application implementation design as well as more futuristic concept development.
A Novel Structure of Variable Inductance High-Frequency Transformer for Power Level Enhancement in Dual-Active-Bridge Converters
Authors: Choi Cheol-Woong , Ko Jae-Sub , Yoon Keun-Yong , Park Yong-Un , Kang Yun-Soo , Kim Dae-Kyong
Published: February 2025

This study presents a novel structure proposal of a variable inductance high-frequency transformer for enhancement power level of a dual-active-bridge (DAB) converter. The DAB converter is a solid-state transformer (SST) that requires high efficiency, power density, flexibility, and stability. Variable inductance facilitates achieving and extending maximum power levels. The proposed method adjusts the inductance based on the core insertion conditions of the transformer used in the DAB converter without requiring an additional inductor. The method was verified by analyzing the variable inductance characteristics of the transformer based on core insertion conditions via finite element analysis (FEA) and simulation and experiments within the variable inductance range.
Characterization, Performance, and Efficiency Analysis of Hybrid Photovoltaic Thermal (PVT) Systems
Authors: Ahmed Md Tofael , Rashel Masud Rana , Islam Mahmudul , Hoque Tania Tanzin , Tlemçani Mouhaydine , Janeiro Fernando M.
Published: February 2025

Hybrid PVT systems simultaneously produce electrical energy using photovoltaic technology and thermal energy using a heat extraction method that collects induced heat from the module. The purpose of this work is to establish a PVT system based on characterization, efficiency study, and performance analysis for both an electrical and a thermal system. A mathematical analysis of the electrical, thermal, and optical model is performed to establish the proposed system. Three types of heat exchanger pipes, including stainless steel, aluminum, and copper, are considered for a heat transfer analysis of the system. The results include temperature profiling, a comparison of the PVT system’s different components, and an overall output and efficiency study for all of the mentioned pipes. Results show that the obtained electrical and thermal efficiency for stainless steel is 0.1653 and 0.237, respectively, for aluminum it is 0.16515 and 0.2401, respectively, and for copper it is 0.16564 and 0.24679, respectively. After comparison, it was found that the overall efficiency for stainless steel is 0.40234, for aluminum is 0.40526, and for copper is 0.41244. Thus, this study will enhance the opportunity to provide an effective hybrid PVT energy management system.
The Properties of Diesel Blends with Tire Pyrolysis Oil and Their Wear-Related Parameters
Authors: Chybowski Leszek , Szczepanek Marcin , Pusty Tomasz , Brożek Piotr , Pełech Robert , Wieczorek Andrzej
Published: February 2025

This research presents the impact of diesel blends with tire pyrolysis oil (TPO) as an additive for minimizing the wear and tear of engine components. This study investigates the blends of normative diesel oil with TPO content ranging from 5% m/m to 20% m/m. Reference measurements are made for pure diesel oil (D100) and pure TPO. This investigation included an evaluation of the corrosion effect and the effect of the fuels tested on abrasive wear. For each fuel, the sulfur content, water content, lubricity (which is defined as the corrected average diameter of the wear trace during the high-frequency reciprocating rig (HFRR) test), and impurity content are determined. Impurities are assessed using indicators such as ash residue, coking residue from 10% distillation residue, determination of wear metals and contaminants, insoluble impurity content, and total sediment by hot filtration. All parameters are determined using recognized methods described in international standards. Approximation models are built for all the analyzed parameters, which can be used in future studies. At the same time, the individual values of the analyzed factors are compared with the threshold values specified in selected standards and regulations. Consequently, it is possible to assess the usefulness of individual fuels in terms of meeting the requirements for minimum wear of engine components. The results show the suitability of pyrolysis oil and the potential for its use as an additive to fossil fuels in terms of meeting most factors. Some of the fuels tested did not meet the standards for acceptable sulfur content. However, in terms of sulfur content, all of the analyzed fuels can be used to power watercraft and land-based power and thermal power plants equipped with flue gas desulphurization systems. A second indicator for not meeting the standards is the ash residue value, which indicates the high content of non-combustible, mainly metallic, substances in the pyrolysis oil used for the tests. Post-recycled oils must, therefore, undergo appropriate purification before being used as an additive to diesel fuels for internal combustion engines. Once the post-recycling oil has been subjected to desulfurization and advanced filtration, it can be used as a fuel additive for land vehicles, which fits in with closed-loop economies and sustainable development strategies.
Detection and Classification of Abnormal Power Load Data by Combining One-Hot Encoding and GAN–Transformer
Authors: Yang Ting , Yu Hongyi , Lu Danhong , Bai Shengkui , Li Yan , Fan Wenyao , Liu Ketian
Published: February 2025

The explosive growth of power load data has led to a substantial presence of abnormal data, which significantly reduce the accuracy of power system operation planning, load forecasting, and energy usage analysis. To address this issue, a novel improved GAN–Transformer model is proposed, leveraging the adversarial structure of the generator and discriminator in Generative Adversarial Networks (GANs). To provide the model with a suitable feature dataset, One-hot encoding is introduced to label different categories of abnormal power load data, enabling staged mapping and training of the model with the labeled dataset. Experimental results demonstrate that the proposed model accurately identifies and classifies mutation anomalies, sustained extreme anomalies, spike anomalies, and transient extreme anomalies. Furthermore, it outperforms traditional methods such as LSTM-NDT, Transformer, OmniAnomaly and MAD-GAN in Overall Accuracy, Average Accuracy, and Kappa coefficient, thereby validating the effectiveness and superiority of the proposed anomaly detection and classification method.
Characterization and Anti-Aging Potency of Phenolic Compounds in Xianhu Tea Extracts
Authors: Zhang Guangwen , Jiang Wenwen , Hu Qing , Luo Jianming , Peng Xichun
Published: February 2025

The health benefits of tea are primarily attributed to its chemical composition, particularly phenolic compounds. As a renowned tea from Guangdong, China, Xianhu tea (XHT) has not been thoroughly studied in terms of its phenolic composition or health-promoting properties. This study characterized the phenolic compounds in Xianhu tea water extract (XHT) using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and evaluated its antioxidant activity in vitro. Furthermore, the effects of XHT extracts on reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), lipofuscin levels, and lifespan in Caenorhabditis elegans were assessed, alongside their modulation of aging-related genes. Compared with Xinyang Maojian tea water extract (XYMJ) and Yingde black tea water extract (YDBT), XHT exhibited a significantly higher polyphenol content, with 23 phenolic compounds identified as characteristic markers. XHT demonstrated superior 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) inhibition capacities, the greatest reductions in ROS, MDA, and lipofuscin levels, and the highest upregulation of SOD and CAT activities. The lifespan was 23.50% and 21.07% significantly longer than nematodes in the control group. Moreover, XHT modulated 13 aging-related genes, which strongly correlated with the 23 identified phenolic compounds. The research results of the above indicators were all obtained without affecting the normal feeding and reproductive capabilities of C. elegans. These findings suggest that these phenolics are the key bioactive components responsible for the anti-aging effects of XHT.
Biochemical and Morphological Traits of Wild Myrtle Populations for Horticultural Use
Authors: Shahbazian Donya , Karami Akbar , Gruda Nazim S.
Published: February 2025

Myrtle (Myrtus communis L.), an evergreen shrub belonging to the Myrtaceae family, is widely valued for its applications in the cosmetic, hygienic, and medicinal industries. This plant produces berries in two colors—white and black—with black berries receiving increasing attention due to their superior antioxidant properties. This study investigates black myrtle berries’ morphological and biochemical characteristics from eight prominent natural habitats in Fars Province, southwestern Iran. The results reveal significant variations in the morphological (such as fruit length and diameter, fruit length-to-diameter ratio, seed length and diameter, fruit weight, pulp weight, seed weight, pulp-to-seed ratio, and number of seeds) and biochemical attributes among the studied populations. The longest fruit was from the Kherqeh (KH) (8.29 mm) population, while the shortest was from the Baghnari (BN) (5.85 mm) population. The largest fruit diameter was also from KH (6.83 mm), which did not show a significant difference compared to the Zanjiran (ZF) population, while the smallest fruit diameter was from BN (5.12 mm), which did not differ significantly from the Kavar (KA), Simakan (SM), Kouhmareh Road (JK), or Atashkadeh (AT) populations. Notably, all populations exhibited high levels of phenolic compounds, ranging from 660 to 1846 mg per 100 g of fresh weight, and potent antioxidant activity, as indicated by low half-maximal inhibitory concentrations ranging from 0.018 to 0.187 mg per gram. Correlation analyses further demonstrated that altitude and specific soil properties influenced the biochemical traits of the berries to varying degrees. These findings offer valuable scientific insights for selecting and utilizing specific myrtle populations in horticulture, particularly for breeding programs to maximize antioxidant properties and phenolic content.
Knowledge and Prevalence of Latent Tuberculosis Infection: A Feasibility and Pilot Study in a Primary Healthcare Setting in Rural Eastern Cape, South Africa
Authors: Magwaza Cebo , Laguda-Akingba Oluwakemi , Apalata Teke , Faye Lindiwe Modest
Published: February 2025

Latent tuberculosis infection (LTBI) remains a significant global health concern, particularly in regions with high tuberculosis (TB) prevalence, such as South Africa. This pilot study aimed to evaluate the prevalence of LTBI and assess patient knowledge about the condition in a primary healthcare clinic in rural Eastern Cape, South Africa. A cross-sectional design was used, and convenience sampling recruited outpatients aged 18 years and older with no prior history of TB. Blood samples were analyzed using the QuantiFERON-TB Gold assay to determine LTBI status, and a survey assessed patient knowledge of LTBI. Strong positive correlations were observed between what patients understand by the term LTBI and how LTBI differs from TB (0.70), what patients understand by the term LTBI and the risk factors for developing LTBI (0.70), how LTBI differs from TB and the risk factors for developing LTBI (0.78), and how LTBI differs from TB and the recommended treatments for LTBI (0.79), indicating overlap in understanding. In contrast, there were negative correlations between if patients had ever heard of latent LTBI before and their understanding of the term LTBI (−0.25), the risk factors for developing LTBI (−0.22), LTBI progressing to active TB (−0.27), and the recommended treatments for LTBI (−0.27). This divergence points to different dimensions of patient knowledge and awareness. Age, gender, occupation, comorbidities, and HIV status showed varying LTBI positivity trends. Among younger patients aged 20–29, 15.4% tested positive, while the 30–39 group showed a nearly equal split between positive (48.1%) and negative cases. A higher positivity rate was seen in females (39.1%) compared to males (31.6%). Unemployed individuals had higher positivity rates, suggesting socioeconomic factors’ influence. Comorbidities, especially hypertension, diabetes, and asthma, correlated with higher LTBI positivity among females, but this was less evident in males. HIV-positive patients had a higher LTBI-negative rate compared to HIV-negative patients. A logistic regression model (accuracy 70%) identified demographic and health factors predicting LTBI outcomes, with comorbidities, particularly hypertension and diabetes, significantly increasing the likelihood of LTBI positivity. These findings suggest that demographic and health factors, including age, gender, occupation, comorbidities, and HIV status, may predict LTBI positivity.
Perspective to Practice: Theoretical Frameworks Explaining Intergenerational Trauma, Violence, and Maltreatment and Implications for the Therapeutic Response
Authors: Bowe Crysta , Thomas Cate , Mackey Patricia
Published: February 2025

Intergenerational trauma, violence, and maltreatment, in which symptoms or experiences of an ancestor’s trauma repeat or otherwise manifest in subsequent generations, presents a weighty societal challenge to which a multiplicity of therapeutic intervention strategies have been applied. Theoretical perspectives are antecedent to clinical and social intervention, informing decisions in both policy and practice. However, these frequently remain subliminal or imperceptible in the discourse, resulting in interventions that remain somewhat dislocated from their theoretical foundations. This narrative review seeks to summarize and discuss each of these theories as they apply to intergenerational trauma, violence, and maltreatment, and to reveal their potential association with specific intervention models or approaches. It positions flexibility between theories and the integration of theories as opportunities to reach new and enhanced understandings and to engender distinctive therapeutic interventions. An enriched understanding of the theories explaining intergenerational trauma, violence, and maltreatment, a deeper appreciation for the pertinence of theory for practice, and an incitement to blend theoretical perspectives in unique ways is, herewith, reached.
Cardiovascular Disease in HIV Patients: A Comprehensive Review of Current Knowledge and Clinical Implications
Authors: Șoldea Sorina , Iovănescu Maria , Berceanu Mihaela , Mirea Oana , Raicea Victor , Beznă Maria Cristina , Rocșoreanu Alexandru , Donoiu Ionuț
Published: February 2025

Cardiovascular involvement in patients with human immune deficiency (HIV) has gained significant attention as the improved life expectancy of individuals with HIV has changed the paradigm regarding the long-term impact of the virus on cardiovascular health. We reviewed current literature on the prevalence, diagnosis, and unique characteristics of cardiovascular disease (CVD) in HIV patients, including those treated with protease inhibitors (PIs) and complementary therapies. The incidence of infectious, immunosuppressive, and nutritionally related pathologies in HIV patients has declined, largely due to advancements in highly active antiretroviral therapies (HAART) and supportive care. However, issues related to autoimmunity and chronic inflammation persist. Elevated levels of high-sensitivity C-reactive protein, along with activated cytokines and other pro-inflammatory molecules, are common in HIV patients and contribute significantly to the increased risk for endothelial dysfunction, coagulation disorders, and accelerated atherogenesis. The advent of HAART has significantly improved the prognosis for HIV patients, leading to prolonged life expectancy and a reduction in AIDS-related complications. However, this success has also resulted in a shift in the clinical presentation, with HIV patients showing more chronic and insidious cardiovascular manifestations.
Omics Approaches in Understanding Insecticide Resistance in Mosquito Vectors
Authors: Bharadwaj Nikhil , Sharma Rohit , Subramanian Muthukumaravel , Ragini Gnanasekar , Nagarajan Shriram Ananganallur , Rahi Manju
Published: February 2025

In recent years, the emergence of insecticide resistance has been a major challenge to global public health. Understanding the molecular mechanisms of this phenomenon in mosquito vectors is paramount for the formulation of effective vector control strategies. This review explores the current knowledge of insecticide resistance mechanisms through omics approaches. Genomic, transcriptomic, proteomic, and metabolomics approaches have proven crucial to understand these resilient vectors. Genomic studies have identified multiple genes associated with insecticide resistance, while transcriptomics has revealed dynamic gene expression patterns in response to insecticide exposure and other environmental stimuli. Proteomics and metabolomics offer insights into protein expression and metabolic pathways involved in detoxification and resistance. Integrating omics data holds immense potential to expand our knowledge on the molecular basis of insecticide resistance in mosquitoes via information obtained from different omics platforms to understand regulatory mechanisms and differential expression of genes and protein, and to identify the transcription factors and novel molecules involved in the detoxification of insecticides. Eventually, these data will help construct predictive models, identify novel strategies, and develop targeted interventions to control vector-borne diseases.
Predictions of the Biological Effects of the Main Components of Tarragon Essential Oil
Authors: Pujicic Andrijana , Popescu Iuliana , Dascalu Daniela , Petreuș David Emanuel , Isvoran Adriana
Published: February 2025

Artemisia dracunculus/(tarragon) is a perennial plant used in traditional medicine and the food industry. The plant is known to have beneficial effects on health, such as antibacterial, antifungal, antiseptic, carminative, anti-inflammatory, antipyretic, anthelmintic, etc. In this study, the compounds present in the highest concentrations in the essential oils obtained by different extraction methods from tarragon found on the Romanian market were identified by gas chromatography–mass spectrometry. The biological activity of these compounds was predicted using the computational tools ADMETlab3.0, admetSAR3.0, CLC-Pred2.0, and AntiBac-Pred. Also, the main molecular target of these compounds was predicted and the interactions with this protein were evaluated using molecular docking. The compounds identified in high concentrations in the obtained essential oils are estragole, cis-β-ocimene, trans-β-ocimene, limonene, eugenol methyl ether, eugenol acetate, eugenol, caryophyllene oxide, and α-pinene. The absorption, distribution, metabolism, excretion, and toxicity profiles of these compounds show that they are generally safe, but some of them can cause skin sensitization and respiratory toxicity and are potential inhibitors of the organic anion transporters OATP1 and OATP2. Several of these compounds exert antibacterial activity against some species of Staphylococcus, Streptococcus, and Prevotella. All compounds reveal potential cytotoxicity for several types of cancer cells. These findings may guide further experimental studies to identify medical and pharmacological applications of tarragon extracts or specific compounds that can be isolated from these extracts.
Non-Uptake of Newborn Screening in Planned Homebirth Is Associated with Preventive Health Practices for Infants: A Retrospective Case-Control Study
Authors: Stein-Zamir Chen , Shoob Hanna , Katan Sandra , Verbov Gina , Almashanu Shlomo
Published: February 2025

Universal Newborn Screening (NBS) programs (for endocrine, immunologic and metabolic disorders) are effective in reducing child morbidity and mortality. Despite available health services, NBS is not carried out for some newborns. The contributing factors for this should be explored. In high-income settings, homebirth generally refers to planned birth at home, attended by skilled health professionals. We aimed to assess trends and characteristics of planned homebirths and the uptake of NBS and infant health practices. A retrospective case-control study including 3246 infants compared planned homebirth (cases) to age-matched hospital birth controls. During 2016–2023, 0.56% of livebirths (1623/290,458) in the Jerusalem District (JD), Israel, were planned homebirths. The rate has increased since 2020 (COVID-19 pandemic), 0.45% in 2016–2019 vs. 0.67% in 2020–2023. Homebirth infants had a higher birthweight, lower firstborn rate and higher socioeconomic rank. The overall NBS uptake in homebirths was significantly lower (73.7% vs. 99.5% in hospital births) and declined over time (81.1% in 2016–2019 vs. 68.7% in 2020–2023). Regarding preventive health practices for homebirth infants, the registration rate to Mother and Child Health Clinics (MCHCs) was lower (47.1% vs. 92.8% in hospital births), and routine immunization rates were decreased (DTaP-IPV-HiB3 90.7% vs. 60.1%). The NBS uptake among homebirth infants was significantly associated with MCHC registration and routine immunizations (RR = 4.15, 95%CI 3.3–5.3). NBS uptake in homebirths is considerably lower and is associated with subsequent patterns of preventive health practices. Notably, the national NBS program data also indicate a trend of increase in non-uptake rates. Barriers to NBS for homebirths should be identified and targeted interventions implemented. The trends in national NBS non-uptake necessitate further follow-up, and evidence from successful outreach programs should be reviewed and translated into guidelines for health organizations.
Morpho-Physiological and Molecular Responses to Seedling-Stage Drought Stress in Different Cowpea Cultivars
Authors: Ritte Inocent Paulin , Egnin Marceline , Bernard Gregory Christopher , Mortley Desmond , Idehen Osagie , Okoma Michelle Pamelas , Bonsi Conrad
Published: February 2025

Drought poses a significant challenge to cowpea growth and productivity, necessitating the development of drought-tolerant cultivars through detailed morpho-physiological and molecular analyses. This study evaluated drought stress responses in cowpea cultivars using polypropylene plastic boxes under greenhouse conditions. RT-qPCR was conducted to assess the relative expression of five photosynthetic and abiotic stress-related genes in a subset of seven contrasting cultivars at 7-, 14-, and 28-days post-treatment initiation (DPTI) and 24 h post-rewatering. Drought-stressed plants showed progressive wilting and a declining chlorophyll content, with plant greenness scores ranging from 2.2 (TVu11987) to 4.7 (TVu2428). California Blackeye (72.2%) and TVu11987 (69.4%) had the highest recovery rates, indicating greater drought tolerance, while TVu2428 had the lowest (2.8%). Gene expression analyses revealed significant drought-induced variation across cultivars and time points. Transcript levels were notably higher in drought-tolerant cultivars, particularly at 14 DPTI and 24 h post-rewatering, aligning with the morpho-physiological screening results. However, gene expression declined as the drought severity increased. These results suggest that California Blackeye, TVu11987, Lobia-I-Sefade, K929, and Aloomba were more drought tolerant compared to Mississippi Silver and TVu2428. Future research using transcriptomic profiling could unravel the complex molecular mechanisms of drought responses in cowpeas, providing valuable insights for breeding genotypes with improved resiliency to drought.
Optimizing Outcomes in Mangled Lower Extremity Reconstruction: Insights from a Retrospective Study of 93 Patients and Their Functional Scores
Authors: Düzgün Serdar , Özdemir Mehmet Taner , Manti Nurettin , Ülgen Nuri Koray , Akkurt Mehmet Orçun
Published: February 2025

Background/Objectives: Over the past 25 years, reconstructive techniques and patient management advancements have significantly improved outcomes in mangled lower extremity injuries. Functional results of limb salvage have been demonstrated to surpass those of primary amputations. Developments such as local fasciocutaneous flaps, vacuum-assisted closure, and hyperbaric oxygen therapy have enhanced the reconstructive ladder. Despite progress, the utility of the Mangled Extremity Severity Score (MESS) and Gustilo–Anderson classification remains debated, particularly in their prognostic value for limb salvage decisions. In the study, we aimed to evaluate the outcomes of optimizing mangled lower extremity reconstruction in 93 patients, focusing on their functional scores retrospectively. Methods: This retrospective study analyzed 93 patients treated for mangled lower extremities between January 2015 and October 2022. Patients were assessed for age, gender, injury location, MESSs, Gustilo–Anderson classifications, surgical methods, and functional outcomes using the Lower Extremity Functional Scale (LEFS). Surgical interventions included internal and external fixation, skin grafts, local flaps, muscle flaps, and free tissue transfer. LEFS scores were categorized into disability levels for functional evaluation. Correlations were drawn between LEFS and variables such as MESS, Gustilo–Anderson types, and nerve injuries. Results: Among the 93 patients, 16 had MESSs ≥ 7, and 77 had MESSs < 7. Reconstruction methods included local fasciocutaneous and muscle flaps (37 patients), free tissue transfer (29 patients), and skin grafting with vacuum-assisted closure (27 patients). Smoking was associated with delayed union and increased infection rates. LEFS scores were significantly lower in patients with MESSs ≥ 7, Gustilo grade 3C fractures, and tibial nerve injuries. Flap failures and a higher number of surgeries (>3) also correlated with poorer functional outcomes. The average soft tissue healing time was 18 days, and bone union time was 17 weeks. Conclusions: Lower extremity reconstruction demands precise surgical planning and execution, prioritizing functional restoration. MESSs and Gustilo–Anderson classifications provide practical frameworks but have limitations in predicting long-term functionality. Factors such as joint involvement, nerve injuries, and flap selection significantly influence outcomes. Smoking and delayed healing remain critical challenges. While free flaps are essential for complex defects, more straightforward methods yield better outcomes in suitable cases. LEFS emerged as a reliable tool.
Prevalence and Risk Factors of Mobile Screen Dependence in Arab Women Screened with Psychological Stress: A Cross-Talk with Demographics and Insomnia
Authors: Gammoh Omar , Alqudah Abdelrahim , Al-Ameri Mariam , Sayaheen Bilal , Alsous Mervat , Al-Tawalbeh Deniz , Alnasraween Mo’en , Muhaissen Batoul Al. , Aljabali Alaa A. A. , Shilbayeh Sireen Abdul Rahim , Qnais Esam
Published: February 2025

Background/Objectives: The current study aims to investigate the rate and the factors associated with mobile screen dependence as a coping mechanism among women residing in Jordan and screened for stress, with a focus on demographics and insomnia. Methods: This cross-sectional study with predefined inclusion criteria used validated tools to assess stress, anxiety, and insomnia. Results: The data analyzed from 431 women showed that 265 (61.5%) were ≤25 years old, 352 (81.7%) received a university education, and 201 (46.6%) were current students. In addition, 207 (48.0%) reported a dependence on mobile screens for coping, 107 (24.8%) reported severe anxiety, and 180 (41.7%) reported severe insomnia. The multivariable regression analysis revealed that mobile screen dependence—as a personal coping choice—was significantly associated with “students” (OR = 1.75, 95% CI = 1.19–2.57, p = 0.004) and “severe insomnia” (OR = 1.07, 95% CI = 1.07–2.32, p = 0.02). Conclusions: We report that a high rate of mobile dependence is associated with students and insomnia. Prompt action should be taken to raise awareness regarding the proper coping mechanisms in this population.