bluuebunny/crossref_metadata_2025 huggingface dataset:
Agroforest diversity and ethnobotanical aspects in two villages of Berau, East Kalimantan, Indonesia
Authors: HARTOYO ADISTI PERMATASARI PUTRI , SUPRIYANTO SUPRIYANTO , SIREGAR ISKANDAR Z. , THEILADE IDA , PRASETYO LILIK B.
Published: October 2018

Hartoyo APP, Supriyanto, Siregar IZ, Theilade I, Prasetyo LB. 2018. Agroforest diversity and ethnobotanical aspects in two villages of Berau, East Kalimantan, Indonesia. Biodiversitas 19: 387-398. REDD+ aims to reduce emissions from deforestation and forest degradation, conservation, enhancement of forest carbon stocks, and sustainable forest management (SFM). An assessment of agroforest is important for REDD+ implementation in Indonesia. In this context, evaluation of the existing agroforests and their uses is an important issue. The objectives of this research were to characterize agroforest diversity with respect to habitat, regulation, production and information functions in Kampung Birang and Kampung Merabu, Berau, East Kalimantan. This research focused on two size classes of trees, namely medium trees (10≤dbh≤20 cm) and large trees (dbh>20 cm). Local community members were trained in plot establishment (60 plots, 50x50 m), tree diameter measurement, trees tagging, and herbarium collection. Local community members identified tree species by local names and listed the species uses. Results showed that agroforests as habitat obtained diversity indices (H’) of moderate category for Kampung Birang and high category for Kampung Merabu. Otherwise, Margalef’s index (Dmg) for the medium and large tree classes in Kampung Birang and Kampung Merabu were classified as high species richness category. This finding implies that enrichment planting using high economic value species is still necessary in Kampung Birang. Kampung Merabu was found to be more diverse than Kampung Birang, although soil quality in Kampung Birang was better than that in Kampung Merabu. Disturbances due to human activities that potentially impact agroforest stability were also pre-identified. The top ten tree species were classified based on importance value index (IVI) across both villages consisting of 80% shade intolerant and 20% semi-tolerant species. Agroforest showing production and information function as produces various edible fruits that are considered as key component for maintaining biodiversity sustainability and ecosystem functioning.
Alarming call from Mursala Island, North Sumatra, Indonesia: The urgent task of conserving the previously reported extinct of Dipterocarpus cinereus
Authors: RACHMAT HENTI HENDALASTUTI , SUBIAKTO ATOK , WIJAYA KESUMA , SUSILOWATI ARIDA
Published: October 2018

Rachmat HH, Subiakto A, Wijaya K, Susilowati A. 2018. Alarming call from Mursala Island, North Sumatra, Indonesia: The urgent task for conserving the previously reported extinct of Dipterocarpus cinereus. Biodiversitas 19: 399-405. IUCN Red List (1998) declared that the Mursalan endemic Dipterocarpus cinereus was extinct in the wild, but brief exploration conducted in the first quarter of 2013 found that there were few numbers of D. cinereus still growing in the island. The objectives of this research were: to describe the current condition of the forest in Mursala island; and to determine the vegetation composition and association between D. cinereus and other major tree species in site. Identifying forest condition and the potential value of the non-timber product was done descriptively through direct observation and interview with local inhabitants. The vegetation analysis was carried out by a purposive stratified technique in three lines each consisting of 4-8 plots (quadrats). The size of each sampling plot was 1,000 m2 (r= 17.8 m) with the total of 20 plots. The result showed that D. cinereus is still growing in the middle part until the top of the ridges. Importance Value Index (IVI) for the top five species were 32.37, 39.47, 17.43, 15.18 and 11.04 respectively for D. cinereus, Shorea acuminata, Arenga pinnata, Shorea falcifera and Hopea sangal. Among those five dominant species, only D. cinereus with S. falcifera and D. cinereus with A. pinnata had a significant association. Our study also determined that calling for species conservation is alarming rate and those not only needed for D. cinereus alone but island ecosystem as a whole.
Autecology and morphological properties of sandalwood (Santalum album) in Pidie District, Aceh, Indonesia
Authors: NUROCHMAN DEDEN , MATANGARAN JUANG RATA , SANTOSA GUNAWAN , SUHARJITO DIDIK , SARI RITA KARTIKA
Published: October 2018

Nurochman D, Matangaran JR, Santosa G, Suharjito D, Sari RK. 2018. Autecology and morphological properties of sandalwood (Santalum album) in Pidie District, Aceh, Indonesia. Biodiversitas 19: 406-412. IUCN Red List (1998) reported that Santalum album L. as an endangered species. Studies on autecology, association, and the utilization of sandalwood on its natural habitat becomes important to perform conservation of the species. This research was conducted in Teungku Dilaweung Village, Pidie District, Province of Aceh. Vegetation analysis was conducted to determine the population structures. Distribution pattern was analyzed using Morisita Index (Id) and Chi-square test, while interspecific association pattern was determined through contingency table, Jaccard Index (JI) and variance-ratio (VR) test. Observation and analysis of physical and chemical properties of soil, topography, and microclimate were done to identify ecological factors affecting the presence of sandalwood. The study showed that the highest abundance at tree level (IVI>100%) was obtained by Syzygium cumini with a density of 15 trees/ha. S. album was found to have clumped distribution pattern with an Id value of 2.258 and χ2compute of 62.571. S. Album has an interspecific association with two species i.e.: Syzygium cumini (χ2compute of 5.104 and JI of 0.846) and Ziziphus oenopolia (χ2compute of 6.516 and JI of 0.833) with VR of 0.474. Interspecific association indicated that S. album has strongly associated with Syzygium cumini and Ziziphus oenopolia. Morphological characters and habitat properties of sandalwood in research site has a similarity to the Province of NTT.
Traditional markets and diversity of edible plant trading: Case study in Ujung Berung, Bandung, West Java, Indonesia
Authors: ISKANDAR BUDIAWATI SUPANGKAT , ISKANDAR JOHAN , IRAWAN BUDI , PARTASASMITA RUHYAT
Published: October 2018

Iskandar BS, Iskandar J, Irawan B, Partasasmita R. 2018. Traditional markets and diversity of edible plant trading: Case study in Ujung Berung, Bandung, West Java, Indonesia. Biodiversitas 19: 437-452. Traditional markets are where traders and buyers meet; places where the supply and demand activities of selling and buying between traders and buyers occur. Buying and selling activities are realized based on the practice of bargaining, made possible by a negotiated willingness to slide a price. In bargaining, social relationships are activated. Traditional markets are managed by local companies called PD Pasar. The traders are generally small business groups. A particular feature of traditional markets is that they are primarily places to trade various foodstuffs that are needed by urban dwellers, including products such as rice and other additional staple foods, vegetables, spices and fruits. The aim of the study reported here was to detail various edible plant species and their variations (landraces) that are the source of products traded in a typical traditional market of West Java, Indonesia. The products of interest to us included carbohydrate staple foods, vegetables, spices, and fruits, produced by village farmers. We investigated the trading network for these edible plant commodities; and the role of traditional markets in supporting the conservation of biodiversity in the edible plants traded. The method used in this study was qualitative, applying an ethnobotanical approach. Field techniques of direct observation, participant observation and deep interview were applied. The results of the study showed that the traditional market of Ujung Berung, in Bandung, West Java, plays an important role in trading various edible plants produced by village farmers. Altogether, 120 plant species were recorded in the market, out of a total of 188 variants (species, and intra-species landraces), representing 44 families. There were 103 species that provided vegetables, 58 species used as spices, 39 species used for their fruits, and 10 that provided carbohydrate staple foods. In general, these plants commodities traded in the Ujung Berung traditional market are supplied by village traders or are bought from the central market in Bandung. The traditional market of Ujung Berung, Bandung has an important role as a place of economic activity for small businesses Furthermore, it is a factor supporting biodiversity in the edible plants traded.
Strategy of the Outer Baduy community of South Banten (Indonesia) to sustain their swidden farming traditions by temporary migration to non-Baduy areas
Authors: ISKANDAR BUDIAWATI SUPANGKAT , ISKANDAR JOHAN , PARTASASMITA RUHYAT
Published: October 2018

Iskandar BS, Iskandar J, Partasasmita R. 2018. Strategy of the Outer Baduy community of South Banten (Indonesia) to sustain their swidden farming traditions by temporary migration to non-Baduy areas. Biodiversitas 19: 453-464. The Baduy people live in the forest village of Kanekes, in Leuwidamar sub-district, Lebak district, Banten Province, Indonesia. They have long practiced a form of swidden farming based on traditional ecological knowledge and beliefs about the cosmos. Unlike the rest of the surrounding Sundanese society, the Baduy have prohibited wet rice field cultivation on their lands. Moreover, in conserving their traditional swidden farming system, the Baduy people have resisted the use of modern rice varieties, inorganic fertilizers, and synthetic pesticides. On the basis of Baduy culture, their swidden-produced rice is not sold, and for at least the past 50 years the harvested rice has not been stored away in barns but instead has been mainly used for daily home consumption and various traditional rituals. Nowadays, Baduy swidden farming is affected by many new pressures – particularly by population increase, and increasing food demand – but the forest land used for practicing their swidden farming is limited. Therefore, in order to maintain the sustainability of their swidden farming system, the Baduy people, particularly the Outer Baduy, have developed cultural strategies that include temporary outmigration (nganjor) to neighboring, non-Baduy areas where Muslim value-systems provide the main cultural dynamic. The aim of the study reported in this paper was to elucidate the cultural strategy of the Outer Baduy to maintain their sustainable swidden farming by temporary out-migration to non-Baduy areas in territory of the Muslim majority. The method used in this study was qualitative based on an ethno ecological approach. Several techniques were used, namely direct observation, participant observation, and deep interviews with competent informants who were purposively selected. The results of the study confirmed that up until now the Outer Baduy have maintained the sustainability of their swidden farming, by various means, especially by conducting contemporary outmigration to non-Baduy areas in Muslim majority territory. They plant swidden rice in a way that fulfills various traditional rituals, and by diversifying their non-rice-trading options that include growing a variety of mainly tree crops in their swidden land (huma) and in other anthropogenic land. This involves occasional negotiated out-migration to non-Baduy area for temporary swidden farming in surrounding Muslim majority territory. This has enabled the Outer Baduy swidden farming system to be maintained on a long-term basis.
Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia
Authors: HARDI ESTI HANDAYANI , NUGROHO RUDY AGUNG , SAPTIANI GINA , SARINAH RIA , AGRIANDINI MAULINA , MAWARDI MIRA
Published: October 2018

Hardi EH, Nugroho RA, Saptiani G, Sarinah R, Anggraidini M, Mawardi M. 2018. Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia. Biodiversitas 19: 480-488. This research was conducted to isolate, identify, and characterize pathogenic bacteria from feces and water of tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture from two selected locations in Samarinda, East Kalimantan, Indonesia. Bacteria were cultured and isolated on NA, TSA, and BHIA media at 30oC for 24 h. Colonies of the isolated bacteria were characterized morphologically in terms of their shape, chromogenesis, edge, and size. Antibiotic sensitivity test on each bacterial isolate was completed using inhibition zone tests. Commercial antibiotics used in this research were nitrofurantoin, ciprofloxacin, oxytetracycline, chloramphenicol, nalidixic acid, gentamicin, and norfloxacin. Koch’s postulates test was done by intraperitoneal injection of bacterial suspension to tilapia (15 g weight) at 103-109 CFU mL-1 in triplicates to determine the pathogenicity of each bacterium. Overall, there were 37 isolates obtained from different sources and growth media that belonged to 14 species: Acinetobacter calcoaceticus (1 isolate), Aerococcus urinae (2 isolates), Aerococcus viridans (1 isolate), Aeromonas hydrophila (1 isolate), Citrobacter freundii (5 isolates), Enterobacter amnigenus (2 isolate), Enterobacter cloacae (4 isolates), Escherichia coli (3 isolates), Listeria sp. (1 isolate), Niseria sp. (4 isolates), Pantoea spp. (1 isolate), Pseudomonas aeruginosa (1 isolate), Staphylococcus aureus (9 isolates), and Streptococcus iniae (2 isolates). Sixteen of the isolates were grown in BHIA medium, 12 isolates in TSA medium and 9 isolates in NA medium. The highest mortality was found in tilapia injected with Enterobacter sp., Listeria sp. and Streptococcus sp. at a density of 109 CFU mL-1. However, the number of bacteria causing mortality in fish was approximately 104-108 CFU mL-1. All bacteria detected in the tilapia and channel catfish cultures were also known as putative pathogens in human.
Short Communication: New records of aphid species Subfamily Aphidinae (Hemiptera: Aphididae) in West Java, Indonesia
Authors: MAHARANI YANI , HIDAYAT PURNAMA , RAUF AUNU , MARYANA NINA
Published: October 2018

Maharani Y, Hidayat P, Rauf A, Maryana N. 2018. Short Communication: New records of aphid species Subfamily Aphidinae (Hemiptera: Aphididae) in West Java, Indonesia. Biodiversitas 19: 510-515. Aphids (Hemiptera: Aphididae) are an economically important group of insects. Their feeding not only damages crops directly but also transmits viruses that cause plant disease. The total number of aphid species recorded in West Java, Indonesia between 1917 and 2013 was 184 species. The aim of the study reported here was to identify and record any aphid species and their host plants in West Java Province, Indonesia that had not previously been recorded. The study was conducted in thirteen districts in West Java. Fourteen species of aphid were newly recorded as found in West Java; five species from the tribe Aphidini, and nine species from the tribe Macrosiphini.
Shallow water sponges along the south coast of Java, Indonesia
Authors: HADI TRI ARYONO , HAFIZT MUHAMMAD , HADIYANTO HADIYANTO , BUDIYANTO AGUS , SIRINGORINGO RIKOH MANOGAR
Published: October 2018

Hadi TA, Hafizt M, Hadiyanto, Budiyanto A, Siringoringo RM. 2018. Shallow water sponges along the south coast of Java, Indonesia. Biodiversitas 19: 535-543. Sponges are the most diverse benthic filter feeders, occupying many different types of marine habitat. The south coast of Java is one such marine habitat, very exposed to the open sea. This study investigated the sponge diversity as well as their morphological characters across the south coast of Java. The observations were carried out from 2011 to 2016 in four different locations, including Pamang Peuk, Gunungkidul, Prigi Bay and Bayuwangi. The study found 96 sponge species, from 15 orders, and described them in terms of nine morphological characters. The most common species included Spheciospongia inconstans, Stylissa massa, Callyspongia sp. and Cinachyrella australiensis, while the most common growth forms were massive and encrusting, accounting respectively for 34.4% and 28.1% of the total number of species. There was a significant difference in the number of species found between sub-tidal and intertidal habitats; subtidal sponges were approximately 50% more diverse than intertidal sponges. Apart from the habitat types, the number of sponges varied in relation to the longitude; east Java had more sponge species with more variations in morphology compared to central and west Java. Encrusting and globular growth forms were the most common characteristics of intertidal sponges in west and central Java, while other growth forms comprised the diverse characteristics of the subtidal sponges in the east Java. This baseline information is essential for management of marine biodiversity hotspots in taking decisions for marine life conservation, because the global trajectory of marine habitat degradation is predicted to rise.
Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia
Authors: HASMIWATI HASMIWATI , SUPARGIYONO SUPARGIYONO
Published: October 2018

Hasmiwati, Supargiyono. 2018. Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia. Biodiversitas 19: 552-558. Aedes aegypti, the primary vector of Dengue fever, is widely distributed in Indonesia including in West Sumatra. Chemical insecticide is an effective way of shedding the chain of Dengue fever transmission. Long-term applications of insecticides have resulted in the development of resistance in Ae. aegypti populations. Knockdown resistance (kdr) allele as a result of point mutations has been found in Voltage-Gated Sodium Channel (VGSC) gene. This study aimed to design allele-specific primers to detect the kdr allele mutations. Ae aegypti samples were collected from several dengue endemic areas in West Sumatra. The susceptibility of adult mosquitos to insecticides was determined by bioassays using impregnation paper test with 0.18% malathion and 0.75% permethrin. In this study, we successfully confirmed that the A. populations have point mutations in the VGSC gene corresponding to S989P and V1016G amino acid substitutions. To genotype S989P and V1016G alleles, we designed kdr allele-specific primers based on the sequence of VGSC gene (NCBI Accession number No. EU399179.1. PCR analysis using the kdr allele-specific primers successfully identified the genotype of Ae. aegypti populations resistant to malathion and permethrin in West Sumatra.
Diversity, potential and conservation of Annonaceae in Bogor Botanic Gardens, Indonesia
Authors: HANDAYANI TRI
Published: October 2018

Handayani T. 2018. Diversity, potential and conservation of Annonaceae in Bogor Botanic Gardens, Indonesia. Biodiversitas 19: 591-603. The Annonaceae is a large family of trees, shrubs and climbers consisting of about 109 genera and 2440 species. This family has important economic value for fresh fruit, cosmetics and perfumes, timber, traditional medicines, processed foods and beverages, insecticides, and ornamental plants. It also has an important role in forest ecosystems and in environmental management, as a key habitat organism. Despite its many uses, the family has not received special attention, and many of its species have been forgotten. Therefore, efforts are required to conserve the Annonaceae. Conservation of the Annonaceae depends upon ex-situ as well as in-situ methods. Conservation by ex situ approaches is considered effective because it can save from extinction species that are rare, endemic or of as yet recognized, unknown usefulness.The research reported in this paper aimed to determine Annonaceae diversity in Bogor Botanic Gardens (BBG), West Java, Indonesia as well as its current and potential utilization, with a view to developing ways of ensuring preservation of the collection within the Gardens. The study used direct observation of the Garden’s collection, compilation and scrutiny of relevant data from the long-term BBG registration database, analysis of past research data, and a literature study. Currently, BBG holds in its collection about 28 genera and 83 species of the Annonaceae family. The collection is estimated to cover 80% of the total species scattered across the Malesian region. Within the collection there are 49 tree species (59.04%), 14 shrub species (16.87%) and 20 liana species (24.09%). Most of the collections are from Java and Sumatra, while the fewest are from Maluku Islands and Papua. Among 40 species observed, 7 species are used for their fruit, 7 species have cosmetic or perfume uses, 22 species are a source of timber, 34 species have uses in traditional medicine, 6 species are used in the food or beverage industry, 19 species can be used as ornamental plants, 8 species for insecticides, and 4 species for other uses.The Annonaceae collections consist of young and old plants.Plants within the Gardens that are more than 100 years old include trees (7 species), shrubs (3 species) and a liana (1 species).The most widely used parts for traditional medicine are roots and leaves. This paper discusses the efforts taken by BBG to conserve the Annonaceae, include maintaining the diversity of the collection, researching the potential usefulness of the collection and exploring different approaches to its conservation.
The effect of understory plants on pollinators visitation in coffee plantations: Case study of coffee plantations in West Bandung District, West Java, Indonesia
Authors: WITHANINGSIH SUSANTI , ANDARI CLARISA DITY , PARIKESIT PARIKESIT , FITRIANI NURULLIA
Published: October 2018

Withaningsih S, Andari CD, Parikesit, Fitriani N. 2018. The effect of understory plants on pollinators visitation in coffee plantations: Case study of coffee plantations in West Bandung District, West Java, Indonesia. Biodiversitas 19: 604-612. West Java is one of the excellent producers of the most expensive coffee in the world (Kopi Luwak). Coffee (Coffea spp.) is one of the crops that require insect pollination for fruit formation. Coffee production in Indonesia is declining in recent years despite the expansion of coffee cultivation area that increases by 2-5 times. The decline in coffee production has been associated with fewer visitations of pollinator insects. Visitations of pollinator insects can improve the quality and quantity of coffee, mainly from the abundance and diversity of pollinator insect. Abundance and diversity of pollinator insects can be improved by to providing them with flowering plants such as understory plants. This study aimed to prove the effect of the existence of flowering plants on the visitation of pollinator insects. Pollinator insects visiting coffee flowers were observed with an observation method in two observation units. The first unit was the location with understory plants while the second was the location without understory plants. The results showed that the abundance of pollinator insects visiting coffee flowers in unit 1 was significantly (P<0.05) higher than that in unit 2. However, the diversity of pollinator insects between the two locations did not differ significantly (P> 0.05).
Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia
Authors: NURHATIKA SRI , ERMAVITALINI DINI , SAPUTRO TRIONO BAGUS , APRIYATMOKO YUDI
Published: October 2018

Nurhatika S, Ermavitalini D, Saputro TB, Apriyatmoko Y. 2018. Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia. Biodiversitas 19: 627-632. Microalgae are aquatic unicellular or multicellular microscopic photoautotroph organisms. High lipid content in microalgae biomass can be used as raw material for biodiesel. Porong river estuary is one of the sites of industrial waste disposal. The polluted waters considered will affect the lipid microalgae metabolism. The main research aims are to isolate and characterize of high lipid content microalgae found in Porong river estuary through the selection of qualitative lipid content. Sampling was conducted at three different stations. Isolation was conducted in solid agar media enriched with Porong River Estuary sterile water and using streak plate method. The isolated microalgae were characterized using a light microscope and to be matched with identification books. The lipid content was determined qualitatively by coloring the isolated-microalgae cells with Nile Red, an intracellular lipid dye then observed its luminescence color under a fluorescence microscope. Several genera of microalgae that can be isolated in this study are Oscillatoria, Nitzschia, Merismopedia, Navicula, Nannochloropsis, and Melosira. The results of qualitative lipid analysis show that the genus Nannochloropsis and Nitzschia have high intracellular lipid accumulation.
Utilization of Araceae by local community in Cisoka Village, Cikijing Sub-district, Majalengka District, West Java, Indonesia
Authors: MUTAQIN ASEP ZAINAL , FATHARANI MUTHI , ISKANDAR JOHAN , PARTASASMITA RUHYAT
Published: October 2018

Mutaqin AZ, Fatharani M, Iskandar J, Partasasmita R. 2018. Utilization of Araceae by local community in Cisoka Village,  Cikijing Sub-district, Majalengka District, West Java, Indonesia. Biodiversitas 19: 640-651. Various species of Araceae plants are widely used by the tribes of Sunda as food. However, cultivation is increasingly rare inWest Java region, and is found in some places only. One area where many people still grow Araceae plants is Cisoka Village, Cikijing Sub-district, Majalengka District, West Java Province, Indonesia. This paper aims to explain the reason for the cultivation of Araceae by the local community in the Village of Cisoka. This study used a combination of qualitative and quantitative methods, but the former was used more than the latter The primary data were collected through structured and semi-structured interviews, participant observation, exploration, and plant sample collection. The data were analyzed descriptively with emic and ethic approaches. The results of the study showed that 20 species and 13 varieties (landraces) of Araceae plants were used by the community. The parts of plant traditionally used by the commumity were tuber, petiole, and leaf. The utilization of the Araceae plants were categorized into 4 main functions, namely, as food, medicine, decoration, and fish feed. The species of Araceae were commonly cultivated in various agroecosystem types, mainly home gardens, gardens, and rice fields.
Estimation of aboveground tree biomass Toona sureni and Coffea arabica in agroforestry system of Simalungun, North Sumatra, Indonesia
Authors: LATIFAH SITI , MUHDI MUHDI , PURWOKO AGUS , TANJUNG ETIKA
Published: October 2018

Latifah S, Muhdi, Purwoko A, Tanjung E. 2018. Estimation of aboveground tree biomass Toona sureni and Coffea arabica in agroforestry system of Simalungun, North Sumatra, Indonesia. Biodiversitas 19: 670-675. Agroforestry is an ecologically and environmentally sustainable land use that offers great promise to carbon (C) sequestration. Forests play a significant role in reducing greenhouse gas emissions through maintaining current carbon stores and by increasing the rate of carbon sequestration. Vegetation carbon stocks are necessary to be quantified to evaluate the carbon sequestration potential in the ecosystem. Reasonable methods for estimating tree biomass and carbon storage on forest land are increasingly crucial given concerns of global climate change. This study aimed to evaluate C sequestration potential by agroforestry in North Sumatra Indonesia. This study was conducted at the Agroforestry system in Aek Nauli, Simalungun District, North Sumatra. Data collection for primary data was done through a field survey. The present study was carried out to determine above ground tree biomass of Toona sureni (Blume) Merr and Coffea arabica. Data retrieval of T. sureni and C. arabica was done by non-destructive sampling by measuring the diameter at breast height (dbh). The results showed that the potential of average above-ground biomass and carbon storage of T. sureni and C. arabica was 6.25 t ha-1 and 2.88 C t ha-1, respectively. Total aboveground biomass of Toona sureni and C. arabica in the study area was 93.75 ton, while total of carbon storage was 43.16 ton
Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene
Authors: AGUNG PASKAH PARTOGI , PUTRA WIDYA PINTAKA BAYU , ANWAR SAIFUL , WULANDARI ARI SULISTYO , SAID SYAHRUDDIN , ZEIN MOCH SYAMSUL ARIFIN , SUDIRO ADITYA
Published: October 2018

Agung PP, Putra WPB, Anwar S, Wulandari AS, Zein MSA, Said S, Sudiro A. 2018. Novel Single Nucleotide Polymorphisms in the Sumba Ongole  (Bos indicus) Growth Hormone Gene. Biodiversitas 19: 676-681. The Sumba Ongole cattle  (Bos indicus) is one of the local Indonesian cattle breeds and has excellent potential to gain higher carcass yield compared to other local cattle breeds in Indonesia. The Growth Hormone  (GH) gene was a potential target for molecular study due to its importance as growth regulating hormone. This study was aimed to determine Single Nucleotide Polymorphism  (SNP) profile of the GH gene in the Sumba Ongole  (SO) cattle using PCR and DNA sequencing methods from 34 individual DNA samples. The results from this study showed that there are 28 SNPs identified within the GH gene; Twenty one SNPs were found to occur in the intron region, 4 SNPs in the exon region, and 3 SNPs in the 3’ end region. There were 17 SNPs in the SO cattle GH gene were caused by novel mutation. The novel SNP g.1395insC was found only in the SO cattle. Moreover, the frequency of the SNP g.1395insC was the highest among other novel SNPs in the SO cattle GH gene. The moderate PIC value  (0.25<PIC<0.50) were found in two novel SNPs i.e. SNP g.1415C>G  (intron 4), and SNP g.1526A>G  (intron 4). The GH gene in the SO cattle was polymorphic and the 17 novel SNPs need to be further validated.
Morphology, growth and genetic variations of sago palm (Metroxylon sagu) seedlings derived from seeds
Authors: RIYANTO RIBUT , WIDODO IMAM , ABBAS BARAHIMA
Published: October 2018

Riyanto R, Widodo I, Abbas B. 2018. Morphology, growth and genetic variations of sago palm (Metroxylon sagu) seedlings derived from seeds. Biodiversitas 19: 682-688. Propagations of Sago palm using seeds will result in large variations of seedlings due to segregation resulting in the genetic diversity. The objectives of this study are to observe the morphology, growth and genetic variations of sago palm seedlings derived from seeds resulted from natural pollination. Morphological diversities of sago palm seedlings showed in high variations with the similarity coefficient ranging from 10% to 69%. Growth patterns of sago palm seedlings were divided into three categories, i.e., slow, medium, and fast growth. Genetic characters of sago palm seedlings showed that sago palm seedlings were varied with coefficients ranging from 23.6-74.5% and seedlings samples were separated into three groups of 49% the differentiation level. Based on the morphological, growth, and genetic characteristics of sago palm seedlings derived from seeds, it is predicted that sago palms in the natural condition may occur because of cross-pollination.
Monitoring orangutan reintroduction: Results of activity budgets, diets, vertical use and associations during the first year post-release in Kehje Sewen Forest, East Kalimantan, Indonesia
Authors: BASALAMAH FITRIAH , ATMOKO SRI SUCI UTAMI , PERWITASARI-FARAJALLAH DYAH , QAYIM IBNUL , SIHITE JAMARTIN , NOORDWIJK MARIA VAN , WILLEMS ERIK , SCHAIK CAREL P. VAN
Published: October 2018

Basalamah F, Utami-Atmoko SSU, Perwitasari-Farajallah D, Qayim I, Sihite J, Van Noordwijk M, Willems E, Van Schaik CP. 2018. Monitoring orangutan reintroduction: Results of activity budgets, diets, vertical use and associations during the first year post-release in Kehje Sewen Forest, East Kalimantan, Indonesia. Biodiversitas 19: 689-700. Pongo pygmaeus morio, a subspecies of orangutan founded east Kalimantan is steadily declining and classified as endangered. A reintroduction program was recently established. We monitored the reintroduced individuals during their first year post-release at Kehje Sewen Forest in East Kalimantan to document the adjustment to their new habitat. Here, we present a report on the activity patterns, food choice, travel height, nest-building abilities and associations of six individuals ranging between eight and thirteen years old. Our results show that all individuals survived their first year. They spent most of their time feeding and had a largely frugivorous diet, similar to wild orangutans. However, although they were able to build nests, they reused or rebuilt old nests more often than expected. They also spent 16% of their total activity time on the ground, more than expected. This information will contribute to attempts to evaluate factors affecting the adjustment process, and thus optimizing future reintroduction procedures.
Molecular characterization of rhizobacteria isolated from walnut (Juglans regia) rhizosphere in Western Himalayas and assessment of their plant growth promoting activities
Authors: DAR GH. HASSAN , SOFI SHAKEELA , PADDER S.A. , KABLI AISHA
Published: October 2018

Dar GH, Sofi S, Padder SA, Aisha Kabli A. 2018. Molecular characterization of rhizobacteria isolated from walnut (Juglans regia) rhizosphere in Western Himalayas and assessment of their plant growth promoting activities. Biodiversitas 19: 712-719. The present study was aimed to isolate and characterize effective bacteria from the rhizosphere of walnut (Juglans regia) grown in North Western Himalayas and assess their growth promoting potential so that they may, in future, be exploited as biofertilizers. Based on preliminary screening of 98 bacterial isolates obtained from four walnut growing districts of Kashmir valley during survey in 2015, 12 isolates were characterized morpho-biochemically and molecularly basis. On the basis of 16S rDNA sequencing they were identified as Bacillus licheniformis WI 90, B. tequilensis WI 62, B. cereus WI 36, B. subtilis strains WI 63 and WI 65, Micrococcus luteus strains WI 12, WI 41 and WI 80; M. yunnanensis strains WI 60 and WI 30 and Micrococcus sp. strains WI 11 and WI 91. The assessment of these rhizobacteria for plant growth promoting attributes revealed that B. licheniformis WI 90 possessed higher phosphorus solubilization activity (312 mg/L), followed by Micrococcus sp. WI 91 (267 mg/L) while high siderophore was produced by M. luteus WI 12 (27.2% siderophore units), followed by B. licheniformis WI 90. B. cereus strains WI 36. High IAA contents (30 µg/mL) was yielded by WI 41, followed by M. yunnanensis WI 60 (28 µg IAA/mL) while higher and statistically at par gibberellic acid was produced by B. licheniformis WI 90, Micrococcus sp. WI 91 and M. luteus WI 80. Higher chitinase enzyme activity was observed in B. subtilis WI 63 (30.5 units/mL), followed by B. tequilensis WI 62 (25.3 units/mL) and B. subtilis WI 65 (25.1 units/mL). The study revealed high plant growth promoting potential in these rhizobacteria.
Biological aspects of Longfin Mojarra (Pentaprion longimanus, Cantor 1849) in north coast of Central Java, Indonesia
Authors: OKTAVIANI DIAN , FAIZAH RIA , NUGROHO DUTO
Published: October 2018

Oktaviani D, Faizah R, Nugroho D. 2018. Biological aspects of Longfin Mojarra (Pentaprion longimanus, Cantor 1849) in north coast of Central Java, Indonesia. Biodiversitas 19: 683-689. Longfin Mojarra (Pentaprion longimanus) locally named as rengganis, is a demersal fish species that is commonly caught in Scottish seine fisheries off the north coast of Java. The fisheries are in heavily harvest level since decades. The aim of this study was to observe the biological aspects of this species. Observations were made between August 2014-July 2015 from Tegal fishing port, western part of north coast central Java. General life-history parameters were measured, i.e., monthly length frequency for 1876 fishes, among them 573 specimens were observed for length-weight relationship, including 541 specimens for sex ratio and maturity stages. Fulton index, Gonadosomatic index, sex ratio and estimated length at first mature were analyzed. The result showed fish size ranged between 7.4 to 15.3 cmFL with the average length of 11.2+1.75 cmFL. We found that length-weight relationship tends to be isometric which statistically the body growth coefficient (b) is equal to 3 (tc = 0.53 < tab; p<0.05) . Monthly Fulton indexes ranged between 1.05-2.87 with an average of 1.90 + 0.17. Sex ratio during observation was not significantly different (p<0.05) from the expected ratio of 1: 1. Mature females occurred year-round with the highest percentage observed from January to April 2015. The estimated length at first maturity was 13.04 cm FL. These biological parameters will be used to support the population study of the future demersal fish species to improve conservation and management measures.
Impact of land and forest fire on soil fauna diversity in several land cover in Jambi Province, Indonesia
Authors: WASIS BASUKI , WINATA BAYU , MARPAUNG DANIEL RICARDO
Published: October 2018

Wasis B, Winata B, Marpaung DR. 2018. Impact of land and forest fire on soil fauna diversity in several land cover in Jambi Province, Indonesia. Biodiversitas 19: 740-746. Forest fire is one of the primary disturbances that damage forest and disturb forest sustainability. Land and forest fire may lead to die-offs, declines and in general influence the development of abundant of soil fauna on the ground. This research aims to identify and assess the diversity, wealth, evenness, and the pattern and spread of land fauna in various ecosystems, both burnt and unburnt by the fire. Research was conducted in some districts in Jambi Province, Indonesia. Sampling methods for soil diversity were done by hand-sorting methods in three burnt and unburnt ecosystems. Those ecosystems were primary forest, industrial plantation forest, and oil palm plantation. Generally, we found 519 individual species included in 46 genera, 33 families, 20 orders of 8 classes, and 3 phyla in whole ecosystem observed. The highest soil fauna diversity was primary forest with value of DMg = 4.243, H'=2.664, E=0.862. Meanwhile, the lowest soil fauna diversity was found in burnt palm plantation with value of DMg=2.102, H'=1.520, and E=0.692. Environmental factors like abiotic and biotic affected to the diversity level of soil fauna.
Morpho-anatomy and physiology of red galangal (Alpinia purpurata) and white galangal (Alpinia galanga) under some salinity stress levels
Authors: SETIAWATI TIA , SUSILAWATI ANIS , MUTAQIN ASEP ZAINAL , NURZAMAN MOHAMAD , ANNISA ANNISA , PARTASASMITA RUHYAT , KARYONO KARYONO
Published: October 2018

Setiawati T, Susilawati A, Mutaqin AZ, Nurzaman M, Annisa, Partasasmita R, Karyono. 2018. Morpho-anatomy and physiology of red galangal (Alpinia purpurata) and white galangal (Alpinia galanga) under some salinity stress levels. Biodiversitas 19: 809-815. Soil salinity is one of the most urgent issues in agriculture as it can reduce plant growth and productivity. Efforts on development of galangal plant on saline soils are still encountering some obstacles such as lack of information on the galangal types that are tolerant to saline condition. The purpose of this study was to determine morpho-anatomical and physiological responses of red galangal (Alpinia purpurata (Vieill.) K. Schum.) and white galangal (Alpinia galanga (L.) Willd.) to salinity stress treatments. This research used a Randomized Block Design (RBD) assigned in a factorial treatment design of two factors, i.e., (i) species of galangal consisting of two levels i.e., red galangal and white galangal, (ii) NaCl concentration consisting of five levels, i.e., 0‰, 3‰, 6‰, 9‰ and 12‰. Each treatment was three replicates. Observation was done on the growth parameters when the plants showed wilting symptoms. The observed data were subjected to Analysis of Variance (ANOVA), followed by a post hoc Duncan Multiple Range Test (DMRT) at α 5% when the treatment effect was significant. The results showed that salinity stress treatment significantly reduced the plant height increase, leaf area, dry weight, stomata density, chlorophyll content and increased thickness of root epidermis, and proline content in both species of galangal. Red galangal was more tolerant than white galangal.
Identification and characterization of Edwardsiella ictaluri from diseased Pangasius pangasius, cultured in Cirata Lake, Indonesia
Authors: MAWARDI MIRA , JAELANI JAELANI , ZAINUN ZAKKI , MUNDAYANA YUANI , CHILORA BAIRD SAM , HARDI ESTI HANDAYANI
Published: October 2018

Mawardi M, Jaelani, Zainun Z, Mundayana Y, Chilora BS, Hardi EH. 2018. Identification and characterization of Edwardsiella ictaluri from diseased Pangasius pangasius, cultured in Cirata Lake, Indonesia. Biodiversitas 19: 766-772. In January 2016, there were reported extensive mortalities of Pangasius pangasius cultured on cage, in Cirata Lake. Edwardsiella ictaluri is primarily recognized as a disease-causing pathogen in catfish species, causing an economically important bacterial infection hence affecting productivity of aquaculture enterprises in many regions across the E. ictaluri. The samples were 17 fishes collected from organs containing spleen, kidney, and liver. The total samples were 51 organs of fishes. The high bacteria infection prevalence level was found in kidney (29.41%). The gross pathological signs of sample of organs were abnormal sizes and abnormal color including white nodules. Bacteria were identified by conventional biochemical test, polymerase chain reaction (PCR) analysis and nucleotide sequencing. The results showed that P. pangasius cultured in cages in Cirata Lake is positively infected by E. ictaluri strain (accession number KF9071291). Other infection cases by Aeromonas sp. and Plesiomonas shigelloides as co-infection bacteria in P. pangasius were also found.
Short Communication: Cave-dwelling Arthropod community of Semedi Show Cave in Gunungsewu Karst Area, Pacitan, East Java, Indonesia
Authors: KURNIAWAN ISMA DWI , RAHMADI CAHYO , CARAKA REZZY EKO , ARDI TIARA E.
Published: October 2018

Kurniawan ID, Rahmadi C, Caraka RE, Ardi TE. 2018. Short Communication: Cave-dwelling Arthropod community of Semedi Show Cave in Gunungsewu Karst Area, Pacitan, East Java, Indonesia. Biodiversitas 19: 857-866. Arthropods are a major group of animals which have significant roles in maintaining cave ecosystem stability. Semedi is a new show cave, but information about arthropods in this cave was not available. The use of cave as a tourist attraction will bring environmental changes which potentially disturb cave-dwelling arthropod community. This study aimed to measure arthropod diversity and their relation to abiotic factors in Semedi Show Cave. Arthropods were sampled by hand collecting, a combination of pitfall and bait traps, and Berlese extractor. Abiotic factors measured were climatic and edaphic parameters. Sampling was conducted in the 3 zones of Semedi cave (Entrance, Twilight, and Dark). Data were analyzed by calculation of richness (Margalef), diversity and evenness (Shannon-Wiener) indices, cluster and correlation analyses. A total 1095 individuals of arthropods consisting of 102 morphospecies, belonging to 6 Classes, and 19 Orders were sampled during this study. The entrance zone had higher richness and diversity indices (richness=12.80, diversity=3.40) than the twilight zone  (richness=7.85, diversity=3.25) and the dark zone  (richness=5.35, diversity=2.63). Meanwhile, the twilight zone had higher evenness index (0.85) than the entrance zone (0.77) and the dark zone (0.77). Each zone of Semedi cave had different abiotic conditions. Abiotic conditions and Arthropod communities in the twilight and dark zones were more similar to each other than to those of the entrance zone. The statistical analyses showed that there were significant correlations between abiotic factors and arthropod communities. Semedi had various cave-dwelling arthropods. Sustainable management of show cave should be applied to minimize the destructive impact of tourism activities on the cave arthropod community.
Bazzania Gray (Lepidoziaceae, Marchantiophyta) in Central Java, Indonesia
Authors: KHOTIMPERWATI LILIH , KASIAMDARI RINA SRI , SANTOSA SANTOSA , DARYONO BUDI SETIADI
Published: October 2018

Khotimperwati L, Kasiamdari RS, Santosa, Daryono BS. 2018. Bazzania Gray (Lepidoziaceae, Marchantiophyta) in Central Java, Indonesia. Biodiversitas 19: 875-887. Bazzania has the largest species of the family Lepidoziaceae (Marchantiophyta). This genus is abundant in the moist montane forest. Diversity of Bazzania in Java insufficiently reported, especially publications about its diversity in Central Java have never been reported. Therefore this study aimed to explore the diversity of Bazzania in Central Java. Studies of the Bazzania were based on the specimens collected from three mountains in Central Java, i.e. Mt. Lawu, Mt. Ungaran and Mt. Slamet. The observation in the laboratory was done based on the morphological and anatomical feature of the stem, lateral leaf, underleaves (amphigastria) and microphyll. Identification of the species used the existing literature that contains key identification, description or illustration of the Bazzania. Eleven species of Bazzania were identified from Central Java, namely Bazzania calcarata, B. japonica, B. javanica, B. pectinata, B. praerupta, B. serpentina, B. spiralis, B. tridens, B. fauriana, B. perfalcata and B. succulenta. Three new record species, namely B. fauriana, B. perfalcata and B. succulenta are reported for Java. This result is the first report of Bazzania in part of Central Java.
Characterization of abundance and diversity of lactic acid bacteria from Apis dorsata hives and flowers in East Nusa Tenggara, Indonesia
Authors: KARYAWATI AMOR TRESNA , NURAIDA LILIS , LESTARI YULIN , MERYANDINI ANJA
Published: October 2018

Karyawati AT, Nuraida L, Lestari Y, Meryandini A. 2018. Characterization of abundance and diversity of lactic acid bacteria from Apis dorsata hives and flowers in East Nusa Tenggara, Indonesia. Biodiversitas 19: 899-905. Previous research on lactic acid bacteria associated with honey bees has been conducted in temperate arid regions and wet tropical regions. We isolated lactic acid bacteria from giant honey bee (Apis dorsata) hives and flowers in East Nusa Tenggara, Indonesia, an area with a tropical savanna climate. Diversity of lactic acid bacteria was studied using Denaturing Gradient Gel Electrophoresis method. The purpose of this study was to obtain information about the diversity of lactic acid bacteria contained in Apis dorsata hives and flowers from tropical savanna climate. We identified seven Operational Taxonomy Units (OTU) within the hives and flowers. Two OTUs were closely related to LactoBacillus kunkeei strain YH-15 (T), while three others were closely related to Lactococcus lactis subsp. tructae strain L105 (T). This information is important to explore the potential utilization of lactic acid bacteria in maintaining human health.