bluuebunny/crossref_metadata_2025 huggingface dataset:
Conducta del concreto reforzado bajo el efecto de diferentes microclimas
Authors: Cedeño Alda
Published: October 2021

Las edificaciones de concreto ubicadas en ambientes urbano y/o marinos experimentan ataques por agentes externos que ocasionan daños en su estructura, disminuyen la durabilidad y afectan la apariencia física de estas ocasionando gastos de mantenimiento o reparación. Debida a la problemática antes descrita, el presente estudio contempla el análisis a pequeña escala del hormigón expuesto en tres estaciones en Panamá que experimentan diferencias ambientales. La duración de la investigación fue de 1.5 años. Las experiencias aplicadas consistieron en análisis fisicoquímicos, espectroscopia infrarroja de Transformada de Fourier (FTIR) y microscopia electrónica de barrido (SEM). Los laboratorios usados pertenecen a la Universidad Tecnológica de Panamá y el Instituto SMITHSONIAN de Panamá. Los resultados se exponen a 1 y 1.5 años de exposición mediante tablas, microfotografías, y espectros de absorbancia. Aplicar los diferentes métodos fue valioso pues se pudo ver la evolución de los parámetros evaluados en el tiempo.
Mejoramiento del uso de suelo en la agricultura mediante herramientas basadas en optimización
Authors: Castillo Edgar , Delgado Oliver , De León Humberto , Escartin Luis , Saéz Yessica , Collado Edwin
Published: October 2021

El uso adecuado de los recursos naturales en la agricultura representa hoy en día uno de los aspectos más importantes directamente relacionados con la seguridad alimentaria a nivel mundial. De esta necesidad surge la idea del uso de herramientas tecnológicas que permitan al productor utilizar de una manera eficiente los suelos agrícolas. Este trabajo propone el desarrollo de una herramienta basada en optimización para garantizar el uso adecuado del suelo, mientras se maximiza la ganancia del sistema de producción de alimentos. Específicamente, se considera un problema de optimización de programación lineal, donde se toman en cuenta variables tales como costo de inversión/costo por hectárea, capital inicial de inversión, área de siembra disponible, área sembrada de cada producto, y la ganancia neta por hectárea de cada producto. Los resultados obtenidos muestran que la herramienta propuesta alcanza un margen de ganancia mayor si se compara con la siembra empírica, utilizando siempre de manera eficiente el área de producción.
Propuesta metodológica para el diseño del slotting de mercancía en centros de distribución
Authors: Caballero Ricardo , González Ariel
Published: October 2021

La logística es una parte fundamental en la cadena de suministro de cualquier empresa y es por medio de la logística que las empresas pueden poner a disposición de los clientes los productos o servicios que ofrezcan en el tiempo y lugar adecuado. Para que esto sea posible, es necesario resaltar la función que ejerce la logística interna en una empresa. Con la implementación de una metodología para el diseño del slotting, se busca definir las fases, pasos y procedimientos para demostrar la forma adecuada en que la mercancía debe estar distribuida dentro del almacén; de manera que permita disminuir el tiempo de selección de pedidos e incremente la productividad del centro de distribución. Para ello, se hace una revisión de la literatura existente para conocer las bases fundamentales de la logística interna en un centro de distribución y técnicas utilizadas para analizar el slotting e inventario que ayuden a diseñar una metodología apropiada. Finalmente, se evalúa practicabilidad de la metodología propuesta mediante su implementación en un centro de distribución.
TALK SHOW KESEHATAN PENGGUNAAN OBAT YANG BAIK DAN BENAR
Authors: Sambodo Dwi Kurniawati , Efendi Yuli Nurullaili
Published: October 2021

One study was showed that about 50% of patients did not use the medicine correctly, did not regularly, or stopped the medichine prematurely. The most common causes are medicine’s effects, the patient did not feel the benefit of the medicine, or a complicated method of use. According to Anief (1997), although drugs can heal, there are many instances that a person has suffered from medicine poisoning. Therefore it can be said that medicine can be medicinal and also poisonous. Through this activity, it is hoped that there will be an increase in public knowledge about how to use medicine that are effective and safe, and that people can do DAGUSIBU properly. The material presented during this event was the definition of medicine, tips on how to get and use drugs effectively and safely, and the drug DAGUSIBU. The health talk show for the use of effective and safe medicine as expected. seen from the participants who attanded and the enthusiasm of the participants in participating in the talk show.
PENGARUH TERAPI FARMAKOLOGI DAN NON-FARMAKOLOGI TERHADAP PENURUNAN NYERI MENSTRUASI PADA MAHASISWI FAKULTAS FARMASI UNIVERSITAS BHAKTI KENCANA BANDUNG
Authors: Anggriani Ani , Mulyani Yani , Pratiwi Lili Dinda
Published: October 2021

Menstrual pain is a problem that women often complain about in their teens. Menstrual pain (dysmenorrhea) is pain before or during menstruation in the lower abdomen due to uterine cramps. The prevalence of dysmenorrhea in Indonesia is 54% primary dysmenorrhea and 9% secondary dysmenorrhea. Due to the severe pain that is felt during menstruation, 10% of teenage girls are absent from school and work for 3 days each month. This study aims to determine the effect of pharmacological & non-pharmacological therapy on reducing menstrual pain in adolescents. This research is an experimental research with one group pretest-post test design. The research sample used purposive sampling method, with a sample of 76 respondents. Data were analyzed using the Wilcoxon Signed Rank Test & chi-square test. The results showed that the Wilcoxon test with the results of Sig. 0.000<0.05, meaning that there is a difference in the intensity of menstrual pain before & after therapy. So it can be concluded that there is an effect of pharmacological and non-pharmacological therapy on decreasing the intensity of menstrual pain. For the relationship of non-pharmacological therapy Chi-Square test results have a value of Sig. 0.514>0.05, meaning that there is no significant relationship to the intensity of menstrual pain. For the relationship between pharmacological therapy, the results of Sig. 0.019<0.05, meaning that there is a significant relationship with the intensity of menstrual pain in adolescent girls at Bhakti Kencana University, Bandung.
Tratamento de sementes com abamectina e Paecilomyces lilacinus no manejo de Heterodera glycines na cultura da soja
Authors: Alves de Almeida Janaina , Cramenak de Souza Jean , Araújo Fernando Godinho de
Published: October 2021

A soja é a mais importante oleaginosa cultivada mundialmente, sendo o Brasil o segundo maior produtor. Para manutenção de índices satisfatórios de produtividade é necessário o manejo eficiente de diversos problemas fitossanitários que afetam a cultura, dentre eles os causados por nematoides. Identificar novas alternativas de manejo desses nematoides faz-se necessário para manutenção de baixos níveis populacionais nas áreas com a presença desses parasitas. Assim, o presente estudo teve como objetivo avaliar a eficiência do tratamento de sementes com abamectina isolada e associada com o fungo Paecilomyces lilacinus no manejo de Heterodera glycines na cultura da soja. O ensaio foi conduzido em delineamento inteiramente casualizado em esquema fatorial 4 (doses de abamectina) x 2 (com e sem a adição de P. lilacinus), com 5 repetições, empregando a cultivar de soja BRS Valiosa RR, suscetível ao nematoide, em ambiente protegido. As doses de abamectina empregadas foram 0, 50, 100 e 200 g. i.a. 100 kg sementes-1 e de P. lilacinus, 600 g p.c. 100 kg sementes-1 (7,5x109 UFC/g de PC). Aos 30 dias após a inoculação foram avaliadas a massa fresca das raízes, o número de fêmeas por sistema radicular, fêmeas por grama de raiz e ovos por fêmea do nematoide. A massa fresca das raízes e o número de fêmeas por sistema radicular foram superiores nos tratamentos sem a adição do fungo P. lilacinos. O tratamento empregando o fungo P. lilacinus apresentou um maior número de fêmeas por grama de raiz quando comparado ao tratamento sem a utilização do fungo. O número de fêmeas por sistema radicular e de ovos por fêmeas reduziram em função das diferentes doses de abamectina utilizadas. A abamectina isolada e associada ao fungo P. lilacinus apresenta potencial de utilização no manejo de H. glycines na cultura da soja.
Análise sensorial de tomates Sweet Grape em diferentes condições de armazenamento
Authors: Cunha Ananda Helena Nunes , Silva Sandra Máscimo da Costa e , Sandri Delvio
Published: October 2021

Dentre as diversas cultivares de tomate, a demanda e a oferta pelo mini tomate têm aumentado nos últimos anos, pois além de ser muito saboroso, é bastante atrativo, sendo muito utilizado na ornamentação de pratos e no preparo de saladas. A utilização de mini tomates como adorno, aperitivo e na confecção de pratos diversos é uma forma de consumo dessa hortaliça, que é bastante consumida em restaurantes por ser considerado uma hortaliça exótica de tamanho pequeno e delicado. Neste contexto, o objetivo do trabalho foi avaliar a vida de prateleira do tomate Sweet Grape produzido em hidroponia com água residuária submetido a diferentes condições de armazenamento. O delineamento experimental foi inteiramente casualizado em esquema fatorial 4 x 2, totalizando oito tratamentos com seis repetições. Os tratamentos foram: quatro dias (0, 7, 13 e 15) e duas temperaturas (refrigerada e ambiente). O tomate foi cultivado em sistema hidropônico com água residuária. Os frutos foram colhidos e acomodados em bandejas poliestireno expandido revestidas com papel filme. Para a análise sensorial utilizou-se Método Afetivo Quantitativo, em que 30 julgadores não treinados, consumidores de tomate, avaliaram os seguintes atributos de qualidade do produto: aparência externa, cor, aparência interna, textura (firmeza) e aroma. No teste de aceitabilidade empregou-se escala hedônica de nove pontos (ancorada em seus extremos por “gostei muitíssimo” e “desgostei muitíssimo”). Os dados obtidos foram submetidos à análise de variância pelo teste F e as comparações entre as médias dos tratamentos foram realizadas pelo teste de Tukey a 5%. Para os resultados obtidos os parâmetros aparência externa e aroma demonstraram diferença para os dias 13 e 15. Já nos parâmetros de cor e textura os tratamentos não demonstraram diferença estatística. A aparência interna demonstrou diferença apenas na temperatura ambiente para os diferentes dias. Desta forma, pode-se concluir que a qualidade do fruto, refletida na análise sensorial para vida de prateleira se mantêm mesmo em diferentes condições de armazenamento e período de estocagem (dias).
CULTIVO DO TOMATEIRO SANTA CRUZ IRRIGADO COM ÁGUA RESIDUÁRIA DOMÉSTICA E ADUBADO COM VERMICOMPOSTO DE LODO DE CURTUME
Authors: Cunha Ananda Helena Nunes , Fernandes Eliana Paula , VIEIRA JONAS ALVES , ARAÚJO FERNANDO GODINHO
Published: October 2021

A solução para o tratamento de resíduos como o lodo de curtume pode ser a vermicompostagem. Manter o potencial nutritivo, para aplicação agrícola como no cultivo de tomate, e também utilizar água residuária doméstica, pode amenizar a contaminação de meio ambiente. Neste contexto, o objetivo do trabalho foi avaliar a produção de tomate Santa Cruz Kada (paulista) irrigado com água residuária doméstica e adubado com vermicomposto de lodo de curtume. O delineamento experimental foi inteiramente casualizado em esquema fatorial 6 x 2, totalizando doze tratamentos com cinco repetições. Os tratamentos foram: quatro vermicompostos de lodo de curtume (25% de Lodo de curtume+Esterco bovino – T1; 25% de Lodo de curtume+Casca de arroz – T2; 50% de Lodo de curtume+Esterco bovino – T3 e 50% de Lodo de curtume+Cinza de cana – T4), adubação convencional (T5) e testemunha (sem adubação – T6) e dois tipos de água de irrigação (água de abastecimento e água residuária). O tomate foi transplantado e conduzido em vasos com volume útil de seis litros, tutorado com estacas de bambu e mantidos duas hastes por planta em cada vaso. Os vermicompostos e o solo utilizados foram caracterizados física e quimicamente, assim como a água residuária doméstica, que foi analisada qualitativamente. Nos frutos de tomate foram realizadas análises de: peso, medidas de diâmetros longitudinal e transversal; pH e oBrix. Os dados obtidos foram submetidos à análise de variância pelo teste F e as comparações entre as médias dos tratamentos foram realizadas pelo teste de Tukey a 5%. Para os resultados obtidos o T2 apresentou maior valor de peso dos frutos assim como de produção (6,68 kg) e diâmetro longitudinal. Para os valores de pH dos frutos de tomate se destaca o T4 com valor de 4,40, o que não difere dos demais tratamentos, com exceção do T1. Para os diferentes tipos de água (A1 e A2) observa-se que os T3 e T5 possuem maiores valores de pH e oBrix. Conclui-se que a produção de tomate pode ser adotada como destinação final da água residuária e do lodo de curtume, resultado em produção satisfatória, principalmente com doses menores de lodo (25%).
Análise das tentativas de suicídio por intoxicações exógenas na região metropolitana de Goiânia, de 2012 a 2017
Authors: Da Silva Sulivan Lemes , Da Costa William Vargas Tenório , Moreira Marília Rodrigues , Oliveira Stefan Vilges de
Published: October 2021

Objetivos: Descrever o perfil epidemiológico das vítimas de intoxicação exógena por suicídio na região metropolitana de Goiânia e propor medidas e propostas de intervenção para a problemática em questão. Método: Trata-se de um estudo transversal, descritivo e quantitativo, em que foram analisados dados do Sistema de Informação de Agravos de Notificação, referente à região metropolitana de Goiânia, abrangendo os casos notificados de intoxicação exógena relacionados ao suicídio no período entre os anos de 2012 a 2017.  Além disso, foram consultadas as plataformas Google Acadêmico e PUBMED e documentos do Ministério da Saúde para busca de referências bibliográficas para subsidiar uma proposta de intervenção. Resultados: No período analisado ocorreram 1830 intoxicações exógenas na região metropolitana de Goiânia, o que corresponde a uma média de 305 casos por ano, sendo o sexo feminino o mais atingido Discussão: Percebe-se que a intoxicação exógena por suicídio é um grave problema de saúde pública e que necessita de medidas eficazes para sua redução, voltadas para indivíduos de todas as localidades, sejam moradores de zonas rurais e urbanas. Medidas para redução dessas intoxicações também deve ser voltada para públicos específicos, de diferentes faixas etárias. Conclusão: Dessa forma, fica evidente a necessidade de construção de políticas públicas que sejam efetivas e que mitiguem as intoxicações exógenas.
Inflammatory parameters in elderly women with and without sarcopenic obesity
Authors: Funghetto Silvana Schwerz , Silva Alessandro Oliveira , Dutra Maurilio Tiradentes , Stival Marina Morato , Barbalho Yuri Gustavo Sousa , Leite Mateus Medeiros , Mota Marcio Rabelo , Lima Luciano Ramos , Silva Izabel Cristina Rodrigues
Published: October 2021

The combination of increased fat mass with a decrease in muscle mass and strength in the elderly has been termed sarcopenic obesity (SO). In addition, augmented levels of inflammatory markers have been reported in subjects with SO. Two hundred-sixteen obese elderly women were included in this cross-sectional study. They underwent body composition analysis by DEXA to define the presence of SO. Volunteers were divided into two groups: SO (n= 83; 66.7 ± 5.6 years) and non-SO (n= 133; 67.6 ± 4.9 years) for the comparison of inflammatory cytokines.  There were no significant differences between SO and non-SO groups in the blood concentrations of the inflammatory markers analyzed, e.g. interleukin-6 (0.82 ± 0.20 vs 0.83 ± 0.19 pg/ml; p=0.64), C-reactive protein (2.70 ± 1.55 vs 2.82 ± 1.66 pg/ml; p=0.71), tumor necrosis factor alpha (0.71 ± 0.08 vs 0.70 ± 0.08 pg/ml; p=0.42) and interferon-gamma (0.75 ± 0.14 vs 0.74 ± 0.08 pg/ml; p=0.47), respectively. Thus, in the studied population, inflammatory markers are not exacerbated by SO when compared to obesity without sarcopenia.
FLUXO POPULACIONAL NA FRONTEIRA FRANCO-AMAPAENSE COMO FATOR IMPORTANTE NA REEMERGÊNCIA DO SARAMPO
Authors: Dos Santos Junior Darci Francisco , Silva Luana Jandira Weber , Dias Marcela Fabiani Silva , Sales Bárbara Luíza Duarte , Brito Victor Hugo Oliveira , Fecury Amanda Alves , De Andrade Rosemary Ferreira
Published: October 2021

Introdução: a Fronteira Internacional, que delimita o Brasil a outros países da América Latina, é local de grande fluxo de migração, possibilitando a disseminação de doenças contagiosas. O sarampo, doença de elevado grau de transmissibilidade e relevância pública, apesar de ser imunoprevenível, demonstrou um pico de incidência importante nessas áreas. Objetivo: evidenciar na literatura científica atualmente disponível, a influência do fluxo populacional para o sarampo como doença reemergente na fronteira internacional franco-amapaense. Material e Métodos: estudo qualitativo de revisão narrativa a partir de artigos indexados nas bases de dados BVS, SCIELO e PERIÓDICOS CAPES durante o mês de maio de 2021, referentes aos últimos 10 anos. Resultados e Discussão: verificou-se pelos dados apresentados a influências das fronteiras na disseminação de doenças contagiosas, devido ao grande fluxo de pessoas em função do turismo, busca por melhores condições de vida e emprego. Na fronteira Franco-Amapaense, esse fluxo migratório está relacionado à extração artesanal de minérios (como o ouro). Além disso, a baixa cobertura vacinal e os movimentos antivacina contribuíram para o ressurgimento do sarampo no Brasil. Conclusão: É importante que os países ajam em redes colaborativas de cuidados à saúde e prevenção, especialmente nas áreas fronteiriças, para evitar o ressurgimento de doenças outrora erradicadas.
Improving Beef Progeny Performance Through Developmental Programming
Authors: Moriel Philipe , Palmer Elizabeth A. , Harvey Kelsey M. , Cooke R. F.
Published: October 2021

Maternal nutritional management during gestation appears to modulate fetal development and imprint offspring postnatal health and performance, via altered organ and tissue development and tissue-specific epigenetics. This review highlighted the studies demonstrating how developmental programming could be explored by beef producers to enhance offspring performance (growth, immune function, and reproduction), including altering cow body condition score (BCS) during pregnancy and maternal supplementation of protein and energy, polyunsaturated fatty acids (PUFA), trace minerals, frequency of supplementation, specific amino acids, and vitamins. However, this review also highlighted that programming effects on offspring performance reported in the literature were highly variable and depended on level, duration, timing, and type of nutrient restriction during gestation. It is suggested that maternal BCS gain during gestation, rather than BCS per se, enhances offspring preweaning growth. Opportunities for boosting offspring productive responses through maternal supplementation of protein and energy were identified more consistently for pre- vs. post-weaning phases. Maternal supplementation of specific nutrients (i.e., PUFA, trace minerals, and methionine) demonstrated potential for improving offspring performance, health and carcass characteristics during immunological challenging scenarios. Despite the growing body of evidence in recent years, the complexity of investigating developmental programming in beef cattle production is also growing and potential reasons for current research challenges are highlighted herein. These challenges include: (1) intrinsic difficulty of accurately measuring cow milk production multiple times in cow-calf systems; (2) larger focus on Bos taurus vs. Bos indicus breeds despite the predominance of Bos indicus-influenced beef breeds in tropical/subtropical environments and their specific, and sometimes opposite, physiological and performance outcomes compared to Bos taurus breeds; (3) limited focus on interaction between prenatal and postnatal management; (4) sex-specific outcomes following similar maternal nutrition during gestation; (5) greater focus on nutrient deficiency vs. excess; (6) limited implementation of immunological challenges; and (7) lack of multigeneration and longer periods of offspring evaluation. This review provides multiple evidence that such obstacles need to be overcome in order to significantly advance the scientific knowledge of developmental programming in beef cattle and promote global beef production.
Predictive Value of Max’s Giant Associated Protein Mutation in Outcomes of Lung Adenocarcinoma Patients Treated With Immune Checkpoint Inhibitors
Authors: Qu Yan , Wang Chao , Liu Lihui , Li Sini , Zhang Xue , Ma Zixiao , Bai Hua , Wang Jie
Published: October 2021

Treatment with immune checkpoint inhibitors (ICIs) has considerably improved prognosis in multiple cancers. However, regardless of PD-L1 expression and TMB, better predictive biomarkers are required to identify ICI-responsive patients. We analyzed a pan-cancer cohort as the discovery cohort to identify the role of Max’s giant associated protein (MGA) mutation in the outcome of ICI treatment in different types of cancers. A pooled lung adenocarcinoma (LUAD) cohort was considered as the validation cohort. Another two LUAD cohorts who received conventional treatment were included for prognostic analysis and mechanism exploration. In the discovery cohort, MGA mutation was a favorable survival biomarker for patients with LUAD than in those with other types of cancers. MGA mutation was positively correlated with the TMB score. The results of the validation cohort were consistent with those of the discovery cohort. Patients with MGA mutation in the TMB-low subgroup had longer survival. Two LUAD cohorts who received standard treatment showed that the MGA mutation was not a prognostic biomarker for standard treatment. Mechanically, we found that the co-mutant genes did not affect the prognostic role of MGA mutation. Gene-set enrichment analysis revealed that genes belonging to the immunodeficiency pathway were enriched in the MGA wild-type group in LUAD. Moreover, activated NK cells were more enriched in the MGA mutant LUAD group. In conclusion, our results demonstrated that MGA mutation was an independent predictive biomarker for ICI therapy. These results may provide a novel insight into identifying potential patients with LUAD for ICI therapy.
Learning the Use of Artificial Intelligence in Heterogeneous Catalysis
Authors: Bokhimi Xim
Published: October 2021

We describe the use of artificial intelligence techniques in heterogeneous catalysis. This description is intended to give readers some clues for the use of these techniques in their research or industrial processes related to hydrodesulfurization. Since the description corresponds to supervised learning, first of all, we give a brief introduction to this type of learning, emphasizing the variables X and Y that define it. For each description, there is a particular emphasis on highlighting these variables. This emphasis will help define them when one works on a new application. The descriptions that we present relate to the construction of learning machines that infer adsorption energies, surface areas, adsorption isotherms of nanoporous materials, novel catalysts, and the sulfur content after hydrodesulfurization. These learning machines can predict adsorption energies with mean absolute errors of 0.15 eV for a diverse chemical space. They predict more precise surface areas of porous materials than the BET technique and can calculate their isotherms much faster than the Monte Carlo method. These machines can also predict new catalysts by learning from the catalytic behavior of materials generated through atomic substitutions. When the machines learn from the variables associated with a hydrodesulfurization process, they can predict the sulfur content in the final product.
Fate of Sc-Ion Interaction With Water: A Computational Study to Address Splitting Water Versus Solvating Sc Ion
Authors: Kumar Nandan , Kumar Y. Bhargav , Sarma Himakshi , Sastry G. Narahari
Published: October 2021

An exhaustive study of Sc-ion interaction with water molecules in all its possible oxidation and spin states has been carried out to delineate the relative propensity of Sc ions toward solvation and water splitting. Potential energy surface analysis of the Sc-ion reaction with water molecules, topological analysis of bonds, and the effect of sequential solvation up to 6 water molecules have been examined. Calculated values showed good agreement with the available experimental results. Close-shell systems such as singlet mono- and tricationic Sc ions prefer to split the water molecules. In contrast, the open-shell systems such as triplet mono- and doublet dicationic Sc ions prefer to get solvated than split the water molecule. Topological analysis of electron density predicted the Sc+/2+–water bond as a noncovalent bond while Sc3+–OH2, Sc2+–OH, and Sc+–H bonds as partially covalent in nature. Energy decomposition analysis revealed that Sc ion–water interactions are driven by electrostatic energy followed by polarization energy. The current study reveals that transition metal catalysis can be one of the most effective tools to employ in water splitting, by properly tuning the electrons, spin, and ligands around the catalytic center.
Preparation and Characterization of Reduced Graphene Oxide /TiO2 Blended Polyphenylene sulfone Antifouling Composite Membrane With Improved Photocatalytic Degradation Performance
Authors: Dai Fengna , Zhang Shangying , Wang Qi , Chen Haiquan , Chen Chunhai , Qian Guangtao , Yu Youhai
Published: October 2021

Nanosized titanium oxide (TiO2)-based photocatalysts have exhibited great potential for the degradation of organic contaminants, while their weak absorption of visible light limits the photocatalytic efficiency. Herein, a novel reduced graphene oxide/TiO2-polyphenylenesulfone (rGO/TiO2-PPSU) hybrid ultrafiltration membrane has been successfully prepared via a non-solvent induced phase-separation method, in which the synergistic coupling between the rGO and TiO2 could endowed the fabricated membranes with visible-light-driven efficient photocatalytically degradation of organic pollutants and outstanding photocatalytic and antifouling properties. Compared with the PPSU membranes prepared with Graphene oxide and TiO2, respectively, the rGO/TiO2-PPSU membrane demonstrated significant photodegradation towards phenazopyridine hydrochloride (PhP) solution under ultraviolet light (improved about 71 and 43%) and visible light (improved about 153 and 103%). The permeability and flux recovery rates of the membrane indicated that the high flux of the rGO/TiO2-PPSU membrane can be greatly restored after fouling, due to the improved self-cleaning properties under visible light static irradiation. With the properties of high performance of photocatalytic degradation and good self-cleaning ability, the rGO/TiO2-PPSU membrane would have great potential in water treatment.
Roseburia Abundance Associates With Severity, Evolution and Outcome of Acute Ischemic Stroke
Authors: Gu Mengmeng , Chen Nihong , Sun Huanhuan , Li Zhongyuan , Chen Xiangliang , Zhou Junshan , Zhang Yingdong
Published: October 2021

Stroke induces disorder of gut microbiota, however, whether this disorder differs according to stroke severity and its role in the evolution and outcome of stroke is currently unknown. Here we explored the composition and structure of fecal microbiome based on 68 acute ischemic stroke patients presenting with minor symptoms (admission National Institute of Health Stroke Scale (NIHSS) ≤ 3) and 67 patients with non-minor stroke (admission NIHSS 4-34) using high-throughput Illumina sequencing of the 16S rRNA. There was no significant difference in α-diversity indices, but the principal coordinate analysis of the microbiota indicated clear separation of the two groups. The significantly enriched butyrate-producing genus Roseburia in the minor stroke group was negatively correlated with fasting glucose, while the Erysipelotrichaceae incertae sedis abundant in non-minor stroke patients was positively correlated with stress hyperglycemia (i.e. fasting glucose/glycated hemoglobin ratio). Moreover, the relative abundance of genus Roseburia was also significantly associated with the dynamic changes of NIHSS score, as well as short-term and long-term functional outcomes. Our results suggested that stroke affects microbiota composition in a manner differentiated by stroke severity, and the enrichment of genus Roseburia may play a protective role in stroke evolution and outcome. Our findings strengthen the relevance of specific taxa for stroke severity that might allow targeted therapy in acute ischemic stroke.
Fecal Microbiota Transplantation Relieves Gastrointestinal and Autism Symptoms by Improving the Gut Microbiota in an Open-Label Study
Authors: Li Ning , Chen Hongyan , Cheng Yi , Xu Fenghua , Ruan Guangcong , Ying Senhong , Tang Wen , Chen Lu , Chen Minjia , Lv LinLing , Ping Yi , Chen Dongfeng , Wei Yanling
Published: October 2021

Autism spectrum disorder (ASD) is a severe brain development disorder that is characterized by deficits in social communication and restricted, repetitive and stereotyped behaviors. Accumulating evidence has suggested that gut microbiota disorders play important roles in gastrointestinal symptoms and neurodevelopmental dysfunction in ASD patients. Manipulation of the gut microbiota by fecal microbiota transplantation (FMT) was recently shown to be a promising therapy for the treatment of various diseases. Here, we performed a clinical trial to evaluate the effect of FMT on gastrointestinal (GI) and ASD symptoms and gut microbiota alterations in children with ASD. We found that there was a large difference in baseline characteristics of behavior, GI symptoms, and gut microbiota between children with ASD and typically developing (TD) control children. FMT could improve GI symptoms and ASD symptoms without inducing any severe complications. Similarly, FMT significantly changed the serum levels of neurotransmitters. We further observed that FMT could promote the colonization of donor microbes and shift the bacterial community of children with ASD toward that of TD controls. The abundance of Eubacterium coprostanoligenes pre-FMT was positively correlated with high GSRS scores, whereas a decrease in Eubacterium coprostanoligenes abundance induced by FMT was associated with the FMT response. Our data suggest that FMT might be a promising therapeutic strategy to improve the GI and behavioral symptoms of patients with ASD, possibly due to its ability to alter gut microbiota and highlight a specific microbiota intervention that targets Eubacterium coprostanoligenes that can enhance the FMT response. This trial was registered at the Chinese Clinical Trial Registry (www.chictr.org.cn) (trial registration number ChiCTR1800014745).
Investigation of Prognostic Markers of Lung Adenocarcinoma Based on Tumor Metabolism-Related Genes
Authors: Zhang Chong , He Zhehao , Cheng Ling , Cao Jinlin
Published: October 2021

Lung adenocarcinoma (LUAD) is a prevalent cancer killer. Investigation on potential prognostic markers of LUAD is crucial for a patient’s postoperative planning. LUAD-associated datasets were acquired from Gene Expression Omnibus (GEO) as well as The Cancer Genome Atlas (TCGA). LUAD metabolism-associated differentially expressed genes were obtained, combining tumor metabolism-associated genes. COX regression analyses were conducted to build a five-gene prognostic model. Samples were divided into high- and low-risk groups by the established model. Survival analysis displayed favorable prognosis in the low-risk group in the training set. Favorable predictive performance of the model was discovered as hinted by receiver’s operative curve (ROC). Survival analysis and ROC analysis in the validation set held an agreement. Gene Set Enrichment Analysis (GSEA), tumor mutation bearing (TMB), and immune infiltration differential analysis were performed. The two groups displayed differences in glycolysis gluconeogenesis, P53 signaling pathway, etc. The high-risk group showed higher TP53 mutation frequency as well as TMB. The low-risk group displayed higher immune activity along with immune score. Altogether, this study casts light on further development of novel prognostic markers for LUAD.
Purinergic Enhancement of Anti-Leishmanial Effector Functions of Neutrophil Granulocytes
Authors: Möller Sonja , Laskay Tamás
Published: October 2021

Although macrophages are considered for host cells for the multiplication ofLeishmania, recent studies indicate the important role of neutrophil granulocytes as host cells for these intracellular parasites. Neutrophils have been shown to be massively and rapidly recruited to the site ofLeishmaniainfection where they represent the first cells to encounter the parasites. Exposure to ATP and UTP have been shown to enhance anti-Leishmaniaactivity of macrophages and intralesional injection of UTP led to strongly reduced parasite loadin vivo. Since thein vivoanti-leishmanial effect of extracellular UTP correlated with enhanced neutrophil recruitment and enhanced ROS production at the site ofLeishmaniainfection we hypothesized that exposure to extracellular nucleotides can directly enhance the killing ofLeishmaniaby neutrophils. Since purinergic signaling is an essential mechanism of neutrophil activation the aim of the present study was to assess whether purinergic exposure results in the activation of anti-leishmanial neutrophil functions and, therefore, represent an essential component of enhanced anti-leishmanial defense in leishmaniasis. We could show that exposure to ATP and UTP led to activation and enhanced CD11b expression of primary human neutrophilsin vitro.Leishmania-induced ROS production was strongly enhanced by extracellular ATP and UTP. Importantly, exposure to ATP and UTP resulted in enhanced killing ofLeishmania donovaniby neutrophils. In addition, ATP strongly enhanced the secretion of IL-8 and IL-1β byLeishmania-exposed neutrophils. Our results suggest that signalingviathe P2 receptor and phosphorylation of Erk1/2, Akt and p38 are involved in the purinergic enhancement of anti-leishmanial functions of neutrophils.
Structure-Size Optimization and Fabrication of 3.7 GHz Film Bulk Acoustic Resonator Based on AlN Thin Film
Authors: Jiang Pingying , Mao Shiping , An Zaifang , Fei Chunlong , Lou Lifei , Li Zhaoxi , Zhao Tianlong , Jiang Shiyi , Yang Yintang
Published: October 2021

Traditional radio frequency filters cannot meet the demands of miniaturization, high frequency operation, integration, and broadband capacity in new-generation communication system owing to their larger volumes. A thin film bulk acoustic resonator (FBAR) is therefore suggested as an optimum solution because of its small volume and a good performance. In this study, the COMSOL multiphysics software was used to build 2 D and 3 D finite element models to analyze the harmonic characteristics of the FBAR. Based on the optimized structural parameters, the FBAR was fabricated with series resonant frequency, parallel resonant frequency, and effective coupling coefficient values of 3.705, and 3.82 GHz, and 7.4%, respectively. Compared with the simulated FBAR results, the effective coupling coefficient of the fabricated FBAR declined by only 0.1%, almost achieving the desired performance.
Identification of the Type IX Secretion System Component, PorV (CHU_3238), Involved in Secretion and Localization of Proteins in Cytophaga hutchinsonii
Authors: Zhao Dong , Song Wenxia , Wang Sen , Zhang Weican , Zhao Yue , Lu Xuemei
Published: October 2021

Cytophaga hutchinsonii can efficiently degrade cellulose and rapidly glide over surfaces, but the underlying mechanisms remain unclear. The type IX secretion system (T9SS) is involved in protein secretion and gliding motility, which is unique to the phylum Bacteroidetes. In this study, we deleted a homologous gene of PorV (chu_3238), a shuttle protein in the T9SS. The Δ3238 mutant caused cellulolytic and gliding defects, while the porV deletion mutants in other Bacteroidetes could glide normally. Adding Ca2+ and K+ improved growth in the PY6 medium, suggesting a potential role of chu_3238 in ion uptake. A proteomic analysis showed an increase in the number of extracellular proteins in the Δ3238 mutant and a decrease in the outer membrane proteins compared to the wild type (WT). Endoglucanase activity in the Δ3238 intact cells was reduced by approximately 70% compared to that of the WT. These results indicate that the secreted proteins could not attach to the cell surface but were released into the extracellular space in the Δ3238 mutant. However, the cargo proteins accumulated in the periplasm of other reported porV deletion mutants. In addition, the homologs of the translocon SprA and a Plug protein were pulled down by co-immunoprecipitation in the 3238-FLAG strain, which are involved in protein transport in the T9SS of Flavobacterium johnsoniae. The integrity of the lipopolysaccharide (LPS) was also affected in the Δ3238 mutant, which may be the reason for the sensitivity of the cell to toxic reagents. The functional diversity of CHU_3238 suggests its important role in the T9SS of C. hutchinsonii and highlights the functional differences of PorV in the T9SS among the Bacteroidetes.
Biological and Molecular Characterization of Two Closely Related Arepaviruses and Their Antagonistic Interaction in Nicotiana benthamiana
Authors: Wang Yaodi , Shen Wentao , Dai Zhaoji , Gou Bei , Liu Hongjun , Hu Weiyao , Qin Li , Li Zengping , Tuo Decai , Cui Hongguang
Published: October 2021

Previously, our group characterized two closely related viruses from Areca catechu, areca palm necrotic ringspot virus (ANRSV) and areca palm necrotic spindle-spot virus (ANSSV). These two viruses share a distinct genomic organization of leader proteases and represent the only two species of the newly established genus Arepavirus of the family Potyviridae. The biological features of the two viruses are largely unknown. In this study, we investigated the pathological properties, functional compatibility of viral elements, and interspecies interactions in the model plant, Nicotiana benthamiana. Using a newly obtained infectious clone of ANRSV, we showed that this virus induces more severe symptoms compared with ANSSV and that this is related to a rapid virus multiplication in planta. A series of hybrid viruses were constructed via the substitution of multiple elements in the ANRSV infectious clone with the counterparts of ANSSV. The replacement of either 5′-UTR-HCPro1–HCPro2 or CI effectively supported replication and systemic infection of ANRSV, whereas individual substitution of P3-7K, 9K-NIa, and NIb-CP-3′-UTR abolished viral infectivity. Finally, we demonstrated that ANRSV confers effective exclusion of ANSSV both in coinfection and super-infection assays. These results advance our understanding of fundamental aspects of these two distinct but closely related arepaviruses.
Clinical Value and Potential Mechanisms of Oxysterol-Binding Protein Like 3 (OSBPL3) in Human Tumors
Authors: Hao Na , Zhou Yudong , Li Yijun , Zhang Huimin , Wang Bin , Liu Xiaona , Ren Yu , He Jianjun , Zhou Can , Tang Xiaojiang
Published: October 2021

Cancer remains one of the top culprits causing disease-related deaths. A lack of effective multi-cancer therapeutic targets has limited the prolongation of cancer patients’ survival. Therefore, it is important to explore novel oncogenic genes or versatile targets and perform a comprehensive analysis to assess their roles in the process of tumorigenesis. OSBPL3 protein is an intracellular lipid receptor of the oxysterol-binding protein superfamily, which participates in some pathological and physiological processes in tumor progression. However, its clinical roles and potential mechanisms in cancers remain unknown. Thus, we aimed to systematic explore the potential oncogenic roles of OSBPL3 across thirty-three tumors using multiple web-based and publicly available tools, including the Cancer Genome Atlas, Gene Expression Omnibus, Genotype-Tissue Expression, cBioPortal, and Human Protein Atlas database. OSBPL3 is highly expressed in major subtypes of cancers, distinctly associated with the prognosis of tumor patients. We observed X676_splice/V676G alteration in the oxysterol domain and frequent mutations of OSBPL3 involve cell survival in skin cutaneous melanoma. We also first presented that the expression of OSBPL3 was associated with tumor mutational burden (TMB) in nine cancer types. Additionally, OSBPL3 shows an enhanced phosphorylation level at S426, S251, and S273 loci within the pleckstrin homology domain in multiple tumors, such as breast cancer or lung adenocarcinoma. And OSBPL3 expression was associated with active immune cells (CD8+ T cells) and cancer-associated fibroblasts in breast cancer, colon adenocarcinoma, and kidney renal clear cell carcinoma and immune checkpoint genes in more than 30 tumors, but weakly associated with immune suppressive cells (myeloid-derived suppressor cells, T regulatory cells). Moreover, protein processing and mRNA metabolic signaling pathways were involved in the functional mechanisms of OSBPL3. Our study first demonstrated that a novel agent OSBPL3 plays an important role in tumorigenesis from the perspective of publicly available databases and clinical tumor samples in various cancers, which comprehensively provide insights into its biological functions and may be helpful for further investigation.
Cerebral Vasoreactivity Changes Over Time in Patients With Different Clinical Manifestations of Cerebral Small Vessel Disease
Authors: Staszewski Jacek , Dȩbiec Aleksander , Skrobowska Ewa , Stȩpień Adam
Published: October 2021

Objectives: Endothelial dysfunction (ED) has been linked to the pathogenesis of cerebral small vessel disease (SVD). We aimed to assess ED and cerebrovascular reactivity (CVR) in the patients with a diverse manifestation of SVD, with similar and extensive white matter lesions (WMLs, modified Fazekas scale grade ≥2), compared with a control group (CG) without the MRI markers of SVD, matched for age, gender, hypertension, diabetes, and to evaluate the change of CVR following 24 months.

Methods: We repeatedly measured the vasomotor reactivity reserve (VMRr) and breath-holding index (BHI) of the middle cerebral artery (MCA) by the transcranial Doppler ultrasound (TCD) techniques in 60 subjects above 60 years with a history of lacunar stroke (LS), vascular dementia (VaD), or parkinsonism (VaP) (20 in each group), and in 20 individuals from a CG.

Results: The mean age, frequency of the main vascular risk factors, and sex distribution were similar in the patients with the SVD groups and a CG. The VMRr and the BHI were more severely impaired at baseline (respectively, 56.7 ± 18% and 0.82 ± 0.39) and at follow-up (respectively, 52.3 ± 16.7% and 0.71 ± 0.38) in the patients with SVD regardless of the clinical manifestations (ANOVA, p > 0.1) than in the CG (respectively, baseline VMRr 77.2 ± 15.6%, BHI 1.15 ± 0.47, p < 0.001; follow-up VMRr 74.3 ± 17.6%, BHI 1.11 ± 0.4, p < 0.001). All the assessed CVR measures (VMRr and BHI) significantly decreased over time in the subjects with SVD (Wilcoxon's signed-rank test p = 0.01), but this was not observed in the CG (p > 0.1) and the decrease of CVR measures was not related to the SVD radiological progression (p > 0.1).

Conclusions: This study provided evidence that the change in CVR measures is detectable over a 24-month period in patients with different clinical manifestations of SVD. Compared with the patients in CG with similar atherothrombotic risk factors, all the CVR measures (BMRr and BHI) significantly declined over time in the subjects with SVD. The reduction in CVR was not related to the SVD radiological progression.
The Correlation Between Olfactory Test and Hippocampal Volume in Alzheimer's Disease and Mild Cognitive Impairment Patients: A Meta-Analysis
Authors: Su Ming-Wan , Ni Jing-Nian , Cao Tian-Yu , Wang Shuo-Shi , Shi Jing , Tian Jin-Zhou
Published: October 2021

Background: Previous studies have reported that olfactory identification deficits may be the earliest clinical features of Alzheimer's disease (AD). However, the association between odor identification and hippocampal atrophy remains unclear.

Objective: This meta-analysis quantified the correlation between odor identification test scores and hippocampal volume in AD.

Method: A search of the PUBMED, EMBASE, and WEB OF SCIENCE databases was conducted from January 2003 to June 2020 on studies with reported correlation coefficients between olfactory identification score and hippocampal volume in patients with amnestic AD or mild cognitive impairment (MCI). The quality of the studies was assessed using the Newcastle-Ottawa quality assessment scale (NOS). Pooled r-values were combined and computed in R studio.

Results: Seven of 627 original studies on AD/MCI using an olfactory identification test (n = 902) were included. A positive correlation was found between hippocampal volume and olfactory test scores (r = 0.3392, 95% CI: 0.2335–0.4370). Moderator analysis showed that AD and MCI patients were more profoundly correlated than normal controls (AD: r = 0.3959, 95% CI: 0.2605–0.5160; MCI: r = 0.3691, 95% CI: 0.1841–0.5288; NC: r = 0.1305, 95% CI: −0.0447–0.2980). Age difference and patient type were the main sources of heterogeneity in this analysis.

Conclusion: The correlation appears to be more predominant in the cognitive disorder group (including MCI and AD) than in the non-cognitive disorder group. Age is an independent factor that affects the severity of the correlation during disease progression. The mildness of the correlation suggests that olfactory tests may be more accurate when combined with other non-invasive examinations for early detection.

Systematic Review Registration:https://inplasy.com/, identifier INPLASY202140088.
Case Report: Covid-19 in Multiple Sclerosis Patients Treated With Ocrelizumab: A Case Series
Authors: De Mercanti Stefania F. , Vercellino Marco , Bosa Chiara , Alteno Anastasia , Schillaci Valentina , Clerico Marinella , Cavalla Paola
Published: October 2021

Introduction: Limited data are available on the course of Coronavirus disease 2019 (COVID-19) in people with Multiple Sclerosis (MS). More real-world data are needed to help the MS community to manage MS treatment properly. In particular, it is important to understand the impact of immunosuppressive therapies used to treat MS on the outcome of COVID-19.

Methods: We retrospectively collected data on all confirmed cases of COVID-19 in MS patients treated with ocrelizumab, followed in two MS Centers based in University Hospitals in Northern Italy from February 2020 to June 2021.

Results: We identified 15 MS patients treated with ocrelizumab with confirmed COVID-19 (mean age, 50.47 ± 9.1 years; median EDSS, 3.0; range 1.0–7.0). Of these, 14 were confirmed by nasal swab and 1 was confirmed by a serological test. COVID-19 severity was mild to moderate in the majority of patients (n = 11, 73.3%; mean age, 49.73; median EDSS 3.0). Four patients (26.7%; mean age, 52.5 years; median EDSS, 6) had severe disease and were hospitalized; one of them died (age 50, EDSS 6.0, no other comorbidities). None of them had underlying respiratory comorbidities.

Conclusion: This case series highlights the large variability of the course of COVID-19 in ocrelizumab-treated MS patients. The challenges encountered by the healthcare system in the early phase of the COVID-19 pandemic might have contributed to the case fatality ratio observed in this series. Higher MS-related disability was associated with a more severe COVID-19 course.