bluuebunny/crossref_metadata_2025 huggingface dataset:
A multi-country survey of the socio-demographic factors associated with adherence to COVID-19 preventive measures during the first wave of the COVID-19 pandemic
Authors: Folayan Morenike Oluwatoyin , Abeldaño Zuñiga Roberto Ariel , Virtanen Jorma I. , Ezechi Oliver C. , Yousaf Muhammad Abrar , Jafer Mohammed , Al-Tammemi Ala’a B. , Ellakany Passent , Ara Eshrat , Ayanore Martin Amogre , Gaffar Balgis , Aly Nourhan M. , Idigbe Ifeoma , Lusher Joanne , El Tantawi Maha , Nguyen Annie L.
Published: July 2023

Background

Health behaviours are influenced by individual characteristics including age, gender, education and economic level. This study aimed to assess the associations between individual-level determinants and adherence to COVID-19 preventive measures.

Methods

We performed secondary analyses of international data collected using an online survey during the first wave of the COVID-19 pandemic between June and December 2020. The dependent variables were self-reported adherence to COVID-19 preventive measures (wearing of face masks, frequent washing/sanitizing of hands, physical distancing, working remotely). The independent variables were age, sex at birth (female vs male), having a chronic disease related elevated risk for severe COVID-19 (none/little, might be at increased risk, at increased risk), educational level completed (no formal education, primary, secondary vs college/university) and employment status (retiree, students, not employed vs employed). Four multivariate logistic regression analyses were conducted to determine the associations between the dependent variables and independent variables. Interaction terms with country-income level were tested in regressions to explore its moderating effect.

Results

Out of 16,866 respondents, 12,634 (74.9%) wore masks or face coverings, 12,336 (73.1%) washed or sanitized their hands frequently, 11,464 (68.0%) reported adherence to physical distancing and 5,646 (33.5%) worked remotely. In adjusted analyses, increased age, college/university education, employment, and having risks for severe COVID-19 were associated with significantly higher odds of adhering to COVID-19 preventive measures. Retirees and students had lower odds of adhering to COVID-19 prevention measures than employed individuals. Males had significantly lower odds of wearing face masks (AOR: 0.901), frequent washing/sanitizing hands (AOR: 0.774) and working remotely (AOR: 0.875) compared to females. Country-income level generally moderated the above relationships such that the associations disappeared in lower income countries.

Conclusion

The study findings suggest that the individual socio-demographic factors—age, sex, employment status, education status and having a chronic disease – influence adherence to COVID-19 preventive measures. Findings further reiterate the need for health education and health promotion campaigns on preventive health measures to focus on subpopulations, such as younger males, students and retirees, that require targeted or unique messaging.
Can locally applied risedronate be an effective agent when combined with xenografts? An animal study
Authors: Özer Taha , Guliyeva Vusala , Aktaş Alper , Barış Emre , Ocak Mert
Published: July 2023

Background

To examine the effects of local risedronate application with xenografts on healing of rabbit skull defects using histological, histomorphometric, immunohistochemical, and three-dimensional radiological methods.

Methods

Two critical-sized defects with a diameter of 10 mm were created in 16 rabbits and filled with xenogenic bone graft and xenogenic bone graft + 5 mg risedronate in the control I and risedronate (RIS) groups, respectively. Residual graft, new bone, soft tissue areas, and bone volume were evaluated in the 4- and 8-week study groups.

Results

In both the 4- and 8-week samples, the RIS group samples had significantly higher mean new bone area values than the C group (p < 0.05). In both groups, the values for the new bone area were significantly higher in the 8-week-old samples than in the 4-week-old samples (p < 0.05). The h scores obtained for sialoprotein and osteopontin did not differ significantly between the groups at either time point (p > 0.05). The results of radiological evaluation showed that the bone density value was significantly higher in the C group than in the RIS group at either time point (p < 0.05).

Conclusions

Although this study aimed to demonstrate the effect of risedronate on the osteoconductive properties of xenografts when applied locally, targeted results could not be achieved.
Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments
Authors: Wang Mengfan , Zhou Yi , Jian Qiuyuan , Ai Zhongzhu , Zhou Shanshan
Published: July 2023

Background

The purpose of this study was to demonstrate the in vitro anti-nephritis activity of Rostellularia procumbens (L.) Nees (R. procumbens) extract and to make a preliminary investigation of its anti-nephritis mechanism.

Methods

A prediction network was built that describes the relationship between R. procumbens and CGN. Then, the potential targets for R. procumbens against CGN were imported into the DAVID database for Gene Ontology (GO) biological annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. A lipopolysaccharide (LPS)-stimulated rat mesangial cell HBZY-1 model in vitro was used to examine the anti-inflammatory activity of R. procumbens extract. RNA-seq was utilized to investigate differentially expressed genes (DEGs) and enriched signaling pathways between groups. Finally, qPCR was used for the validation analysis of the experimental results.

Results

The results of network pharmacology showed that R. procumbens exerts its therapeutic effect on CGN through the AGE-RAGE signaling pathway in diabetic complications, PI3K-Akt, IL-17 signaling pathway, and so on. R. procumbens n-butanol extract (J-NE) can effectively relieve inflammation in HBZY-1. The results of KEGG pathway enrichment suggest that J-NE attenuated CGN was associated with the IL-17 signaling pathway, and the results of RNA-seq were consistent with network pharmacology. Targets enriched in the IL-17 signaling pathway, including Chemokine (C-C motif) ligand 7 (CCL7), Lipocalin 2 (LCN2), Chemokine (C-C motif) ligand 2 (CCL2), and Chemokine (C-X-C motif) ligand 1 (CXCL1), have been identified as crucial targets attenuating CGN by J-NE.

Conclusion

R. procumbens is a promising pharmacological candidate for the treatment of CGN in the present era.
Accessibility to specialist palliative care services in Germany: a geographical network analysis
Authors: Gesell Daniela , Hodiamont Farina , Bausewein Claudia , Koller Daniela
Published: July 2023

Background

The need for palliative care will increase over the next years because of the rise in deaths from chronic illness and demographic changes. The provision of specialist palliative care (SPC) in Germany (palliative care units (PCU), specialist palliative home care (SPHC) teams and palliative care advisory (PCA) teams) has been expanded in recent years. Despite the increasing availability, there is still insufficient coverage with long travel times. The aim was to describe the spatial distribution of SPC services in Germany, to calculate the potential accessibility of facilities and to assess potential spatial under-provision.

Methods

Retrospective cross-sectional study with regional analysis of SPC services in Germany. Addresses of SPC services registered online were geocoded, accessibility and network analyses were conducted, and proportion of the population living up to 60 minutes driving time were calculated.

Results

A total of 673 facilities were included. Their distribution is heterogeneous with every fourth of the 401 districts (110/401; 27.4%) lacking a SPC service. In half of the area of Germany the existing PCU and SPHC teams are within reach of 30 minutes, with nearly 90% of the population living there. Hospitals providing PCA teams can be reached within 30 minutes in 17% of the total area with provision for 43% of the population.

Conclusions

A high coverage of SPHC teams and PCU indicates a good spatial distribution in Germany but no complete adequate provision of SPC services, especially for PCA teams. There is a persistent need for further implementation of hospital PCA teams.
Ex vivo biomechanical evaluation of extracapsular stabilization with quasi-isometric points in canine cranial cruciate ligament-deficient stifles
Authors: Hsu Wei-Ru , Lin Cheng-Chung , Sun Cheng-Yuan , Wu Ching-Ho
Published: July 2023

Background

Cranial cruciate ligament (CCL) disease is one of the most common causes of lameness in dogs. The extracapsular stabilization (ECS) utilizing bone anchors and monofilament nylon leader was an alternative treatment for CCL-deficient (CCLD) dogs. However, the biomechanical response of the canine stifle to such a surgical repair strategy in conjunction with the use of recently reported quasi-isometric anchoring points remains unclear. The objectives of the study were to evaluate the mobility and stability of CCL-intact, CCLD, and CCLD stifles repaired with ECS at two different pairs of quasi-isometric points (quasi-IPs).

Methods

Twelve stifle specimens from 7 dogs underwent mobility and stability tests under 4 different conditions, namely, CCL-intact, CCLD, and ECS-repaired at 2 different pairs of quasi-IPs (referred to as ECS-IP1 and ECS-IP2). The mobility tests evaluated 6 degrees-of-freedom stifle kinematics during flexion and extension. The stability tests involved cranial drawer and tibial internal rotation (IR) tests at various stifle opening angles and quantifying the cranial tibial translation (CTT) and tibial IR angles under constantly applied loadings.

Results

The ECS repaired at quasi-IPs was shown to restore cranial instability of the stifles with averaged CTT magnitudes < 1.4 mm. During the tibial IR test, the ECS treatments resulted in significantly less tibial IR compared to those in intact CCL stifles. The mobility tests showed similar results.

Conclusion

The 2 chosen pairs of quasi-IPs were shown to effectively correct the excessive CTT caused by CCLD stifles, whereas the excessive tibial external rotation in comparison to those of intact stifles should be considered for its subsequent influence on joint alignment and the contact pressure applied to the stifle joint.
The effect of matrices on the gene expression profile of patient-derived head and neck carcinoma cells for in vitro therapy testing
Authors: Hyytiäinen Aini , Korelin Katja , Toriseva Mervi , Wilkman Tommy , Kainulainen Satu , Mesimäki Karri , Routila Johannes , Ventelä Sami , Irjala Heikki , Nees Matthias , Al-Samadi Ahmed , Salo Tuula
Published: July 2023

Objective

Head and neck squamous cell carcinoma (HNSCC) is a highly aggressive tumor with a 5-year mortality rate of ~ 50%. New in vitro methods are needed for testing patients’ cancer cell response to anti-cancer treatments. We aimed to investigate how the gene expression of fresh carcinoma tissue samples and freshly digested single cancer cells change after short-term cell culturing on plastic, Matrigel or Myogel. Additionally, we studied the effect of these changes on the cancer cells’ response to anti-cancer treatments.

Materials/methods

Fresh tissue samples from HNSCC patients were obtained perioperatively and single cells were enzymatically isolated and cultured on either plastic, Matrigel or Myogel. We treated the cultured cells with cisplatin, cetuximab, and irradiation; and performed cell viability measurement. RNA was isolated from fresh tissue samples, freshly isolated single cells and cultured cells, and RNA sequencing transcriptome profiling and gene set enrichment analysis were performed.

Results

Cancer cells obtained from fresh tissue samples changed their gene expression regardless of the culturing conditions, which may be due to the enzymatic digestion of the tissue. Myogel was more effective than Matrigel at supporting the upregulation of pathways related to cancer cell proliferation and invasion. The impacts of anti-cancer treatments varied between culturing conditions.

Conclusions

Our study showed the challenge of in vitro cancer drug testing using enzymatic cell digestion. The upregulation of many targeted pathways in the cultured cells may partially explain the common clinical failure of the targeted cancer drugs that pass the in vitro testing.
20-year trends in multimorbidity by race/ethnicity among hospitalized patient populations in the United States
Authors: Mohamud Mursal A. , Campbell David J.T. , Wick James , Leung Alexander A. , Fabreau Gabriel E. , Tonelli Marcello , Ronksley Paul E.
Published: July 2023

Background

The challenges presented by multimorbidity continue to rise in the United States. Little is known about how the relative contribution of individual chronic conditions to multimorbidity has changed over time, and how this varies by race/ethnicity. The objective of this study was to describe trends in multimorbidity by race/ethnicity, as well as to determine the differential contribution of individual chronic conditions to multimorbidity in hospitalized populations over a 20-year period within the United States.

Methods

This is a serial cross-sectional study using the Nationwide Inpatient Sample (NIS) from 1993 to 2012. We identified all hospitalized patients aged ≥ 18 years old with available data on race/ethnicity. Multimorbidity was defined as the presence of 3 or more conditions based on the Elixhauser comorbidity index. The relative change in the proportion of hospitalized patients with multimorbidity, overall and by race/ethnicity (Black, White, Hispanic, Asian/Pacific Islander, Native American) were tabulated and presented graphically. Population attributable fractions were estimated from modified Poisson regression models adjusted for sex, age, and insurance type. These fractions were used to describe the relative contribution of individual chronic conditions to multimorbidity over time and across racial/ethnic groups.

Results

There were 123,613,970 hospitalizations captured within the NIS between 1993 and 2012. The prevalence of multimorbidity increased in all race/ethnic groups over the 20-year period, most notably among White, Black, and Native American populations (+ 29.4%, + 29.7%, and + 32.0%, respectively). In both 1993 and 2012, Black hospitalized patients had a higher prevalence of multimorbidity (25.1% and 54.8%, respectively) compared to all other race/ethnic groups. Native American populations exhibited the largest overall increase in multimorbidity (+ 32.0%). Furthermore, the contribution of metabolic diseases to multimorbidity increased, particularly among Hispanic patients who had the highest population attributable fraction values for diabetes without complications (15.0%), diabetes with complications (5.1%), and obesity (5.8%).

Conclusions

From 1993 to 2012, the secular increases in the prevalence of multimorbidity as well as changes in the differential contribution of individual chronic conditions has varied substantially by race/ethnicity. These findings further elucidate the racial/ethnic gaps prevalent in multimorbidity within the United States.

Prior presentations

Preliminary finding of this study were presented at the Society of General Internal Medicine (SGIM) Annual Conference, Washington, DC, April 21, 2017.
Nuclear import of Mas-related G protein-coupled receptor member D induces pathological cardiac remodeling
Authors: Zhao Kun , Hua Dongxu , Yang Chuanxi , Wu Xiaoguang , Mao Yukang , Sheng Yanhui , Sun Wei , Li Yong , Kong Xiangqing , Li Peng
Published: July 2023

Alamandine (Ala), a ligand of Mas-related G protein-coupled receptor, member D (MrgD), alleviates angiotensin II (AngII)-induced cardiac hypertrophy. However, the specific physiological and pathological role of MrgD is not yet elucidated. Here, we found that MrgD expression increased under various pathological conditions. Then, MrgD knockdown prevented AngII-induced cardiac hypertrophy and fibrosis via inactivating Gαi-mediacted downstream signaling pathways, including the phosphorylation of p38 (p-P38), while MrgD overexpression induced pathological cardiac remodeling. Next, Ala, like silencing MrgD, exerted its cardioprotective effects by inhibiting Ang II-induced nuclear import of MrgD. MrgD interacted with p-P38 and promoted its entry into the nucleus under Ang II stimulation. Our results indicated that Ala was a blocking ligand of MrgD that inhibited downstream signaling pathway, which unveiled the promising cardioprotective effect of silencing MrgD expression on alleviating cardiac remodeling.
Green and white MEKC for determination of different anti-diabetic binary mixtures and their triple-combo pill
Authors: Ahmed Aya R. , Korany Mohamed A. , Galal Shereen M. , Ragab Marwa A. A.
Published: July 2023

The work introduces green and white sustainable micellar electrokinetic chromatography (MEKC) procedure that could analyze therapeutically related drugs, empagliflozin (EMP), linagliptin (LIN) and metformin (MET) which are antidiabetic drugs with different mechanism of action, in their different pharmaceutical combinations. The method not only comply with the green analytical concepts, but also it is in line with sustainable analytical concepts as it is economic by applying the same operating conditions to analyze different pharmaceuticals in quality control (QC) labs which is a crucial step in QC labs and research centers to save time, effort, and money. Moreover, the method functionality regarding its scope with its achieved levels of accuracy, precision, low detection, and quantitation limits is tested using white assessment tool and compared with reported methods. The proposed MEKC coupled with a diode array detector (DAD) has been developed and validated for micro estimation of EMP and LIN in their low critical concentrations with MET in a ratio of (EMP: MET, 1:40) and (LIN: MET, 1:200). Separation was achieved within 6 min using fused silica capillary (40 cm × 50 µm id) using 20 mM Tris buffer (pH 10) in presence of 50 mM sodium dodecyl sulphate and 10% v/v methanol. The concentration ranges of the studied anti-diabetic drugs were 10–500, 10–100 and 2.5–100 µg. mL−1for MET, EMP and LIN, respectively. The developed method is the first MEKC for concurrent determination of EMP, LIN and MET with high separation efficiency, low solvent consumption and regard as an easy green and white analytical tool. Moreover, Greenness and whiteness assessment were done via the most widely used Analytical Eco-Scale, the innovative AGREE tool and the RGB 12 algorithm.
Thermoplastic starch (TPS)-based composite films for wastewater treatment: synthesis and fundamental characterization
Authors: Abas Khadiga Mohamed , Attia Amina Abdel Meguid
Published: July 2023

Modification of starch is a potential basic research aiming to improve its water barrier properties. The general purpose of this study is to manufacture cross-linked iodinated starch citrate (ISC) with a degree of substitution (DS) ≈ 0.1 by modifying native corn starch with citric acid in the presence of iodine as an oxidizing agent. Thermoplastic starch (TPS) was generated with urea as a plasticizer and blended with various concentrations of ISC of (2, 4, 6%) (wt/wt) to obtain (UTPS/ISC2, UTPS/ISC4, and UTPS/ISC6). Nanocomposite film was formed from UTPS/ISC2 in presence of stabilized iodinated cellulose nanocrystals UTPS/ISC2/SICNCs via gelatinization at a temperature of 80ºC. Water solubility and water vapor release were studied amongst the water barrier features. The fabricated starch-based composite films were evaluated utilizing Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electronic Microscope analysis (SEM), surface area, and tensile measurements. The adsorption of crystal violet (CV) dye onto produced samples was examined in an aqueous solution. The findings revealed that the UTPS/ISC2/ISCNCs has 83% crystal violet elimination effectiveness. Moreover, the adsorption isotherms were assessed and figured out to vary in the order of Langmuir > Temkin > Freundlich > Dubinin-Radushkevich.
Evaluation of levels of uric acid and lipid profile in hospitalized patients with diabetes
Authors: Fayazi Haniyeh Sadat , Mortazavi Khatibani Seyyedeh Sahereh , Motamed Behrang , Yaseri Maryam
Published: July 2023

Objective

Diabetes is the most common metabolic disorder that leads to various complications, and among these complications, disruption in the lipid profile and serum uric acid (SUA) is one of the significant cases that can lead to the deterioration of the health status of patients with diabetes. So, we aimed to evaluate the level of SUA and lipid profiles in patients with diabetes. A total of 230 patients with diabetes who were admitted to Razi Hospital, Rasht, Iran, were enrolled in this study. Demographical data and clinical characteristics of the patients include gender, body mass index (BMI), duration of diabetes, history of smoking, FBS, HbA1c, SUA, Creatinine (Cr), Cholesterol (Chol), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG), retinopathy, hypertension, ischemic heart disease (IHD), and microalbuminuria were recorded. All data were analyzed using the SPSS version 21 by a significant level < 0.05.

Result

According to our results, 70 were male, and 160 were female, with a mean age of 57.36 ± 8.05 years and a mean BMI of 28.10 ± 4.62. The most frequent comorbidities were hypertension, 67%. The serum level of FBS, HBA1c, SUA, Cr, Chol, LDL, HDL, and TG were 191.47 ± 71.66 mg/dL, 7.94 ± 1.21 mg/dL, 5.65 ± 1.95 mg/dL, 0.94 ± 0.16 mg/dL, 167.28 ± 45.22 mg/dL, 95.91 ± 37.03 mg/dL, 39.78 ± 10.44 mg/dL, and 186.75 ± 76.65 mg/dL, respectively. Only UA had a significant relationship with TG level (P < 0.05).
The age at first consumption of forage in calves and its effect on growth and rumination in the short- and long-term
Authors: Xiao Jianxin , Chen Tianyu , Peng Rong , Alugongo Gibson Maswayi , Yang Hui , Khan Muhammad Zahoor , Liu Shuai , Ma Yulin , Wang Jingjun , Wang Wei , Wang Yajing , Li Shengli , Cao Zhijun
Published: July 2023

Background

Previous investigations into the effect of dietary forage on calf performance have been inconsistent, and there is a paucity of information exploring the effect of age on the growth performance and rumination of calves. Eighty-four female Holstein calves (41.5 ± 4.2 kg) were enrolled at birth, a subset of the calves were fed calf starter only (CON, n = 21) while the rest (n = 63) were classified into three treatment groups: the early (EHAY, n = 26, 5.1 ± 0.8 d), the middle (MHAY, n = 21, 7.9 ± 0.8 d) and the late (LHAY, n = 16, 12.1 ± 1.4 d) hay consumers. The short-term effect of the age at first forage consumption (AFF) on calves’ feed intake was monitored until d 84. In addition, the long-term effects of AFF on body weight, structural growth and rumination behavior were recorded until d 196. Rumen samples were collected on d 1, 7, 35, 84 and 196 to analyze the rumen fermentation, while fecal samples were collected from d 78 to 84 to estimate digestibility parameters.

Results

Treatment had no effect on feed intake. While, the EHAY calves tended to have lower BW and ADG compared to LHAY and CON calves. Several total-tract apparent digestibility parameters and digestible nutrients intake were significantly lower in EHAY calves compared with CON and LHAY calves. Calves in the EHAY group tended to begin ruminating ealier, while CON calves were the latest (12.3 vs. 15.5 days of age). A treatment and time interaction was present for rumination time due to greater rumination in calves consuming hay compared to CON calves in week 10 to 12, the differences in rumination disappeared afterwards, no long-lasting significant differences in the rumination and rumen fermentation parameters were found between treatments.

Conclusions

In conclusion, this study showed that hay consumption earlier in life (in the first week, around 5 days of life) could negatively affect the growth of the calf in the short and long term. Compared to consuming hay from the second week (around 12 days of life) or feeding concentrate only without hay, starting to consume hay from the first week could compromise nutrient digestibility and digestible nutrient intake independent of developing rumination behaviour and rumen fermentation.
Microbiota perturbation by anti-microbiota vaccine reduces the colonization of Borrelia afzelii in Ixodes ricinus
Authors: Wu-Chuang Alejandra , Mateos-Hernandez Lourdes , Maitre Apolline , Rego Ryan O. M. , Šíma Radek , Porcelli Stefania , Rakotobe Sabine , Foucault-Simonin Angélique , Moutailler Sara , Palinauskas Vaidas , Aželytė Justė , Sǐmo Ladislav , Obregon Dasiel , Cabezas-Cruz Alejandro
Published: July 2023

Background

Ticks can transmit a broad variety of pathogens of medical importance, including Borrelia afzelii, the causative agent of Lyme borreliosis in Europe. Tick microbiota is an important factor modulating, not only vector physiology, but also the vector competence. Anti-microbiota vaccines targeting keystone taxa of tick microbiota can alter tick feeding and modulate the taxonomic and functional profiles of bacterial communities in the vector. However, the impact of anti-microbiota vaccine on tick-borne pathogen development within the vector has not been tested.

Results

Here, we characterized the Ixodes ricinus microbiota modulation in response to B. afzelii infection and found that the pathogen induces changes in the microbiota composition, its beta diversity and structure of bacterial community assembly. Tick microbiota perturbation by anti-microbiota antibodies or addition of novel commensal bacteria into tick midguts causes departures from the B. afzelii-induced modulation of tick microbiota which resulted in a lower load of the pathogen in I. ricinus. Co-occurrence networks allowed the identification of emergent properties of the bacterial communities which better defined the Borrelia infection-refractory states of the tick microbiota.

Conclusions

These findings suggest that Borrelia is highly sensitive to tick microbiota perturbations and that departure from the modulation induced by the pathogen in the vector microbiota pose a high cost to the spirochete. Network analysis emerges as a suitable tool to identify emergent properties of the vector microbiota associated with infection-refractory states. Anti-microbiota vaccines can be used as a tool for microbiota perturbation and control of important vector-borne pathogens.
Effects of early local administration of high-dose bFGF on a recurrent laryngeal nerve injury model
Authors: Goto Takao , Ueha Rumi , Sato Taku , Yamasoba Tatsuya
Published: July 2023

Background

Research on regenerative medicine using basic fibroblast growth factor (bFGF) has recently advanced in the field of laryngology. We previously reported that local administration of bFGF 1 month after recurrent laryngeal nerve (RLN) paralysis compensated for atrophy of the thyroarytenoid muscle. The objective of this study was to elucidate the effects of early bFGF administration on the thyroarytenoid muscle after RLN transection and to investigate the underlying mechanisms.

Methods

A rat model of RLN paralysis was established in this study. One day after RLN transection, low- (200 ng) or high-dose (2000 ng) bFGF or saline (control) was administered to the thyroarytenoid muscle. The larynges were excised for histological and immunohistochemical examinations at 1, 7, 14, 28, and 56 days after administration.

Results

The cross-sectional thyroarytenoid muscle area was significantly larger in the high-dose group than in the saline and low-dose groups on days 28 and 56. Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56.

Conclusions

A single, early local administration of high-dose bFGF prevented atrophic changes in the thyroarytenoid muscles by activating satellite cell proliferation and reforming neuromuscular junctions. As increased neuromuscular junctions are expected to maintain myofiber volume, bFGF administration may prevent thyroarytenoid muscle atrophy in the mid to long term.

Graphical Abstract
Reactive buffering window trajectory segmentation: RBW-TS
Authors: Zaman Bakht , Altan Dogan , Marijan Dusica , Kholodna Tetyana
Published: July 2023

Mobility data of a moving object, called trajectory data, are continuously generated by vessel navigation systems, wearable devices, and drones, to name a few. Trajectory data consist of samples that include temporal, spatial, and other descriptive features of object movements. One of the main challenges in trajectory data analysis is to divide trajectory data into meaningful segments based on certain criteria. Most of the available segmentation algorithms are limited to processing data offline, i.e., they cannot segment a stream of trajectory samples. In this work, we propose an approach called Reactive Buffering Window - Trajectory Segmentation (RBW-TS), which partitions trajectory data into segments while receiving a stream of trajectory samples. Another novelty compared to existing work is that the proposed algorithm is based on multidimensional features of trajectories, and it can incorporate as many relevant features of the underlying trajectory as needed. This makes RBW-TS general and applicable to numerous domains by simply selecting trajectory features relevant for segmentation purposes. The proposed online algorithm incurs lower computational and memory requirements. Furthermore, it is robust to noisy samples and outliers. We validate RBW-TS on three use cases: (a) segmenting human-movement trajectories in different modes of transportation, (b) segmenting trajectories generated by vessels in the maritime domain, and (c) segmenting human-movement trajectories in a commercial shopping center. The numerical results detailed in the paper demonstrate that (i) RBW-TS is capable of detecting the true breakpoints of segments in all three usecases while processing a stream of trajectory points; (ii) despite low memory and computational requirements, the performance in terms of the harmonic mean of purity and coverage is comparable to that of state-of-the-art batch and online algorithms; (iii) RBW-TS achieves different levels of accuracy depending on the various internal parameter estimation methods used; and (iv) RBW-TS can tackle real-world trajectory data for segmentation purposes.
Embracing a culture of talk: STEM teachers’ engagement in small-group discussions about photovoltaics
Authors: Firetto Carla M. , Starrett Emily , Jordan Michelle E.
Published: July 2023

Background

Small-group discussions are well established as an effective pedagogical tool to promote student learning in STEM classrooms. However, there are a variety of factors that influence how and to what extent K-12 teachers use small-group discussions in their classrooms, including both their own STEM content knowledge and their perceived ability to facilitate discussions. We designed the present study to specifically target these two factors in the context of photovoltaics, an interdisciplinary field at the intersection of all STEM disciplines with potential to yield widespread benefits related to the use of solar technologies as a sustainable, renewable energy source. Teachers engaged in a series of small-group discussions based on photovoltaic source material (e.g., scientific articles) to build both their STEM content knowledge and capability with discussions, promoting their potential to design and deliver STEM instruction in their own classrooms using small-group discussion.

Results

Overall, teachers productively engaged in rich STEM talk as they spent most of the time in the discussion asking authentic questions about photovoltaic topics in alignment with a variety of science and engineering disciplinary core ideas, responding to the questions with rich, elaborative talk, and taking on ownership of the discussions. Teachers also evidenced increases in their photovoltaic knowledge and their perceived capability to facilitate discussions. Finally, most teachers’ end-of-program lesson plans included the use of small-group discussions, and a subsample of teachers who completed a follow-up interview one year after the summer program reported greater enactment of discussion in their STEM classrooms.

Conclusion

Our manuscript forwards an important contribution that draws from a practice-based approach to professional development in a way that not only better prepares teachers on what to teach (i.e., through enhanced PV content knowledge), but it also supports their ability to implement this instruction into their classrooms more effectively (i.e., though the use of small-group discussion). As such, this manuscript illustrates an innovative pedagogical approach for potential use in supporting teacher education and informs ways to enable teachers to build enhanced curricula for their STEM students.
Metagenomic insight into the biodegradation of biomass and alkaloids in the aging process of cigar
Authors: Xue Fang , Yang Juan , Luo Cheng , Li Dongliang , Shi Guiyang , Song Guangfu , Li Youran
Published: July 2023

A significant distinction between cigar production and tobacco lies in the necessary aging process, where intricate microbial growth, metabolic activities, enzymatic catalysis, and chemical reactions interact. Despite its crucial role in determining the final quality of cigars, our comprehension of the underlying chemical and biological mechanisms within this process remains insufficient. Biomass and alkaloids are the primary constituents that influence the flavor of cigars. Consequently, investigating the entire aging process could begin by exploring the involvement of microbes and enzymes in their biodegradation. In this study, handmade cigars were aged under different conditions. Metagenomic sequencing was employed to identify the microbes and enzymes responsible for the degradation of biomass and alkaloids derived from tobacco leaves. The results revealed that various environmental factors, including temperature, humidity, duration time, and turning frequency, yielded varying contents of total sugar and alkaloids in the cigars. Significant correlations were observed between microbial communities and starch, reducing sugars, total sugars, and alkaloids. Key species involved in the breakdown of biomass constituents, such as starch (Bacillus pumilus, Pseudomonas sp. 286, and Aspergillus cristatus), reducing sugars and total sugars (Aspergillus cristatus and Nitrolancea hollandica), were identified. Furthermore, Corynespora cassiicola and Pseudomonas fulva were found to potentially contribute to the degradation of alkaloid compounds, specifically nornicotine and neonicotinoid. Our work contributes to a deeper understanding of the microbial roles in the aging of cigars. Moreover, the selection of specific microbial strains or starter cultures can be employed to control and manipulate the aging process, thereby further refining the flavor development in cigar products.

Graphical Abstract
Contraceptive use pattern based on the number and composition of children among married women in sub-Saharan Africa: a multilevel analysis
Authors: Teshale Achamyeleh Birhanu , Wang Vicky Qi , Biney Godness Kye , Ameyaw Edward Kwabena , Adjei Nicholas Kofi , Yaya Sanni
Published: July 2023

Background

The relationship between composition of children and contraception use has received limited scholarly attention in sub-Saharan Africa. In this study, we examined the relationship between contraceptive methods, the number and composition of children in SSA.

Methods

Data on 21 countries in sub-Saharan Africa (SSA) countries that had a Demographic and Health Survey on or before 2015 were analysed. We applied a multilevel multinomial logistic regression model to assess the influence of family composition on contraceptive use. Adjusted relative risk ratio (aRRR) and 95% CI were estimated. The significant level was set at p < 0.05. All the analyses were conducted using weighted data.

Results

Women who had one son and two daughters (aRRR = 0.85, CI = 0.75, 0.95), two sons and one daughter (aRRR = 0.81 CI = 0.72, 0.92), one son and three daughters (aRRR = 0.66, CI = 0.54, 0.80), two sons and two daughters (aRRR = 0.59, CI = 0.50, 0.69), and three or more sons (aRRR = 0.75, CI = 0.63, 0.91) were less likely to use temporary modern contraceptive methods. Those with two sons and two daughters were less likely to use traditional methods (aRRR = 0.52, CI = 0.35, 0.78). Women in the older age group (35–49 years) were less likely to use temporary modern methods (aRRR = 0.60; 95%CI; 0.57, 0.63). However, this group of women were more likely to use permanent (sterilization) (aRRR = 1.71; 95%CI; 1.50, 1.91) and traditional methods (aRRR = 1.28; 95%CI; 1.14, 1.43).

Conclusion

These findings suggest that contraception needs of women vary based on the composition of their children, hence a common approach or intervention will not fit. As a result, contraception interventions ought to be streamlined to meet the needs of different categories of women. The findings can inform policymakers and public health professionals in developing effective strategies to improve contraceptive use in SSA.
Does the public know what researchers know? Perceived task difficulty impacts adults’ intuitions about children’s early word learning
Authors: Knabe Melina L. , Schonberg Christina C. , Vlach Haley A.
Published: July 2023

The present study examined adults’ understanding of children’s early word learning. Undergraduates, non-parents, parents, and Speech-Language Pathologists (N = 535, 74% female, 56% White) completed a survey with 11 word learning principles from the perspective of a preschooler. Questions tested key principles from early word learning research. For each question, participants were prompted to select an answer based on the perspective of a preschooler. Adults demonstrated aligned intuitions for all principles except those derived from domain-general theories, regardless of experience with language development (Experiment 1). Experiment 2 revealed that perceived difficulty of a task for a preschooler impacted adults’ reasoning about word learning processes. Experiment 3 ruled out level of confidence and interest as mechanisms to explain the results. These results highlight disconnects in knowledge between the cognitive development research community and the general public. Therefore, efforts must be made to communicate scientific findings to the broader non-academic community, emphasizing children’s ability to excel at word learning in the face of task difficulty.
Experiences with and perception of patient-reported outcome measurement in patients undergoing knee and hip replacement in Germany
Authors: König Adriana N. , Himmler Sebastian , Buschner Peter , Sundmacher Leonie
Published: July 2023

Background

Patient-reported outcome measures - PROMs - have been developed to provide an assessment of patients’ physical function, symptoms, and health-related quality of life. With patient-centered care becoming increasingly important, several national strategies have been initiated for PROM measurement. However, Germany is only at the beginning of this process. The objective of this study is to assess patients’ experience with and perception of completing PROMs in patients undergoing knee and hip replacement in Germany.

Methods

This study used survey data from patients undergoing hip or knee replacement surgery in a hospital in Germany. Before surgery, patients completed a PROMs survey. After at least 6 months, patients were re-contacted to fill in a questionnaire about their experiences with and perception of the PROMs data collection.

Results

Most patients either agreed or totally agreed that the time to fill in the questionnaire was appropriate (89%), that the purpose of the PROMs collection was clear (85%), that the questionnaire’s content applied to their appointment (73%), and that this systematic assessment was beneficial (81%). The corresponding proportions were 54% for feeling productive while waiting and 50% for feeling that the information in the questionnaire affected the patient-doctor interaction positively. Only few significant associations were found between patient characteristics and the favorability of patients’ ratings. There were no significant differences between hip and knee replacement surgery patients regarding the favorability rating on any survey question.

Conclusions

The results of this study suggest that PROMs collection in the context of hip and knee replacement surgery is practicable and partly also perceived beneficial by patients. Orthopedic procedures could serve as a starting point for broader use and routine PROMs collection in Germany.
Influence of contrast medium on tophus detection using dual-energy CT: phantom study and clinical illustration
Authors: Kotlyarov Maximilian , Mews Jürgen , Ulas Sevtap Tugce , Ziegeler Katharina , Hamm Bernd , Diekhoff Torsten
Published: July 2023

Background

To investigate the influence of iodinated contrast medium (ICM) on detection of monosodium urate (MSU) with dual-energy computed tomography (DECT) in two types of phantoms and demonstrate an example patient for clinical illustration.

Methods

Approval is by the institutional review board, and written informed consent was obtained. A grid-like and a biophantom with 25 suspensions containing different concentrations of ICM (0 to 2%) and MSU (0 to 50%) were prepared and scanned with sequential single-source DECT using established methodology. Ascending orders of tube currents were applied at 80 kVp (16.5 to 220.0 mAs) and 135 kVp (2.75 to 19.25 mAs). Volume and mass measurements were performed using clinical gout software (dual-energy decomposition analysis). Numbers of true-positive and false-positive MSU detections were recorded and compared for different ICM concentrations. We demonstrate a patient with gouty arthritis for clinical illustration.

Results

Effects of ICM on MSU detection varied with the amount of iodine. Lower ICM concentrations (0.25 and 0.50%) improved detection of small uric acid concentrations of 35 to 45% in comparison to scans without ICM. However, high ICM concentrations (1 and 2%) almost completely precluded MSU detection for all MSU concentrations investigated. In a patient with gouty arthritis, tophi in the wrist were only detected after intravenous ICM administration.

Conclusions

Exploring multimodal DECT for arthritis imaging, enhancement of ICM influences tophus detection. It can help in visualizing previously undetected MSU depositions but, with too strong enhancement, also obscure tophi.

Relevance statement

Use of iodinated contrast media in dual-energy CT might help in visualizing previously undetected uric acid depositions but, with too strong enhancement, obscure gouty tophi.

Key points

• Iodine significantly influences the uric acid crystal detection in systematic phantom studies.

• Lower iodine concentrations improved detection of low and medium uric acid concentrations.

• High concentrations of iodine hampered detection of all uric acid concentrations.

Graphical abstract
Relationship of big five personality traits and future specialty preference among undergraduate medical students: a cross-sectional study
Authors: Sultan Sadia , Labban Osama M. , Hamawi Alyazeed M. , Alnajrani Ali K. , Tawfik Ayat Mahmoud , Felemban Mohammed H. , Bokhari Enad A. , Aziz Nusrat
Published: July 2023

Background

The precise choice of specialty is of paramount importance in a physician’s career. Several factors play a role in deciding the specialty, such as intelligence, motivation, clinical experience, personality, and socioeconomic factors. Our hypothesis is whether personality profiles differ based on specialty choice. Therefore, we investigated the association between personality traits and specialty choice.

Results

A cross-sectional study was conducted among 379 undergraduate medical students. Big five personality inventory was used to determine the personality traits among medical students. The most chosen speciality among both male and females was Surgery. Comparing the groups depending on their preferred specialties revealed no differences in three of five personality traits of Big five personality inventory. Students who selected psychiatry specialty scored highest on openness (p = 0.007) and lowest on extraversion (p = 0.018), indicating a substantial difference between the traits of extraversion and openness to experience.

Conclusions

The differences in personality features could not be attributed to a predilection for a particular speciality. However, medical students who scored higher on openness (intellect/imagination) and less on extraversion scales were more likely to choose psychiatric specialty. Numerous other factors affect specialization preference even if there are no differences in personality features between various specialties.
Geodetic study on earth surface loading with GNSS and GRACE
Authors: Heki Kosuke , Jin Shuanggen
Published: July 2023

Ice, snow, and liquid water on the surface of the Earth exert downward force onto the solid earth and deform the lithosphere typically in seasonal timescale. Space techniques, such as Global Navigation Satellite System (GNSS), made it possible to directly measure subtle displacements caused by loading. We can also observe such loads with time-variable gravity using gravity recovery and climate experiment satellites. These techniques made surface loads an attracting scientific target of modern geodesy. In this paper we briefly review the history of geophysical studies of surface loads through geodetic observations of crustal deformation and time-variable gravity. We also review advanced topics such as short-term crustal deformation due to severe meteorological episodes and monitoring of terrestrial water storages. We also present a few related topics such as the change of the obliquity of the Earth due to loads and artificial crustal subsidence signals caused by snow accretion onto GNSS antenna radomes.
PR and QRS interval changes after transcatheter pulmonary valve replacement in children
Authors: Helal Abdelmonem M. , Baho Haysam A. , Elmahrouk Ahmed F. , Mashali Mohamed H.
Published: July 2023

Background

Changes in PR intervals after transcatheter pulmonary valve replacement (TCPVR) have not been thoroughly evaluated in children. This study evaluated the changes in PR and QRS intervals six months after TCPVR in children with congenital heart disease.

Results

This study included 41 patients who underwent TCPVR from 2010 to 2022. ECG of patients was reviewed before and six months after TCPVR, and the PR and QRS intervals were reported. Right ventricular systolic pressure (RVSP) was retrieved indirectly from echocardiography and compared pre- and 6-months after TPVR. The median age was 13 years (25th–75th percentiles: 11–16), and 61% were males. The preoperative diagnosis was tetralogy of Fallot (n = 29, 71%), transposition of great vessels (n = 4, 10%), common arterial trunk (n = 3, 7%), pulmonary valve stenosis (n = 3, 7%) and pulmonary atresia (n = 2, 5%). The Melody valve was used in 30 patients, and Edwards Sapien was used in 11 patients. RVSP was significantly reduced six months after the procedure (pre-RVSP 40 (30–55) mmHg vs. post-RVSP 25 (20–35) mmHg; P < 0.001). The PR interval was 142 (132–174) msec before TPVR and 146 (132–168) msec post-TCPVR (P = 0.442). Post-TPVR PR was positively related to the pre-PR (β: 0.79 (0.66–0.93), P < 0.001) and inversely related to the right ventricular outflow tract size (− 1.48 (− 2.76 to − 0.21), P = 0.023). The pre-TPVR QRS was 130 (102–146) msec, and the post-TPVR QRS was 136 (106–144) msec (P = 0.668).

Conclusions

In children undergoing TCPVR, the PR and QRS intervals did not change significantly during a 6-month follow-up.