bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychological Climacteric Symptoms and Attitudes toward Menopause among Emirati Women
Authors: Ali Amira Mohammed , Ahmed Afaf Hassan , Smail Linda
Published: July 2020

Menopause is an inevitable developmental event that women encounter at an age of 42–54 years. The drop of estrogen levels that accompanies cessation of menstruation is associated with multiple vasomotor, physical, neuropsychological, and sexual symptoms, which may hamper quality of life. This study aimed to examine the severity of psychological symptoms and their correlates among peri- and postmenopausal Emirati women (N = 60, mean age = 54.88 ± 6 years). Participants were interviewed using the Menopause-Specific Quality of Life (MENQOL) and attitudes toward menopause scale (ATMS). In four path analysis models, vasomotor symptoms, weight gain, and fatigue had significant direct effects on symptoms of anxiety, depression (only weight gain and fatigue), and psychological distress. Fatigue significantly mediated the effects of vasomotor symptoms and weight gain on symptoms of anxiety, depression (only vasomotor symptoms), psychological distress, and memory problems. These models explained 47.6%, 44.5%, 56.6%, and 29.1% of the variances in anxiety, depression, psychological distress, and memory problems, respectively. Participants with more severe menopausal symptoms expressed more negative attitudes toward menopause though regression analysis revealed that only vasomotor symptoms could significantly contribute to ATMS scores. In conclusion, psychological distress is widespread among menopausal women, and it is associated with vasomotor symptoms, fatigue, and change of body composition (obesity). Psychological symptoms, along with vasomotor symptoms, express a key link to negative attitudes toward menopause. Therefore, interventional strategies that target psychological distress may promote coping with midlife transition and improve mental health among menopausal women.
Associations of Social and Psychological Resources with Different Facets of Chronic Stress: A Study with Employed and Unemployed Adolescents
Authors: Wade-Bohleber Laura M. , Duss Carmen , Crameri Aureliano , von Wyl Agnes
Published: July 2020

Adolescents navigate many psychosocial changes. A critical transition in adolescence is the one from school to work life. Both taking the first steps in work life and the failure to achieve this transition and being unemployed can engender elevated levels of stress during adolescence. Stress, especially when experienced chronically, is an important risk factor for mental health problems. Social and psychological resources may mitigate the experience of chronic stress. This study explored associations of social and family support, self-esteem, and self-efficacy with different dimensions of chronic stress in a sample of 1405 employed and unemployed adolescents (M(age) = 17.84, SD = 1.63, range: 14.05–26.12) in Switzerland. Unemployed adolescents showed higher stress levels overall. Higher levels of social and psychological resources were generally linked to lower stress levels. Social support and self-esteem predicted stress levels most consistently and strongly. On several stress dimensions, the association between higher self-esteem and lower stress levels was more pronounced in employed youth whereas the association between higher social support and lower stress levels was stronger in unemployed youth. Our findings provide insights on the differential associations of social and psychological resources with various facets of chronic stress in the context of employment and unemployment during adolescence.
Accuracy of Dynamic Navigation System Workflow for Implant Supported Full Arch Prosthesis: A Case Series
Authors: Stefanelli Luigi V. , Mandelaris George A. , Franchina Alessio , Pranno Nicola , Pagliarulo Michele , Cera Francesca , Maltese Fabio , De Angelis Francesca , Di Carlo Stefano
Published: July 2020

A minimally invasive implant treatment approach for future full arch implant prosthetic rehabilitations of trophic jaws represents a challenge. An optimal implant planning is strongly related with an accurate merge of the prosthetic information and the radiographic data. To comply with that, most computer aided implantology (CAI) systems require additional steps, as radiographic stents or fiducial markers to overlap digital jaw scans to cone beam computed tomography (CBCT) data. Using dynamic CAI, residual teeth (up to three) make it possible for the merge to avoid new radiographic scans. An additional challenge is the treatment involving immediate implants compared with delayed implants placed into healed bone. As for other static CAI systems, the operator’s experience and the quality of the CBCT data make the planning affordable and secure the entire implants placement procedure. The literature reports accuracies in terms of comparison between placed implants and planned implants, following a double CBCT approach, based on radiographic volume overlapping. Thirteen consecutive future totally edentulous patients (77 implants), divided into two groups (group A: 3–4 teeth traced; group B: 5–6 teeth traced) requiring a full arch implant prosthetic rehabilitation were included in the reported case series. A dynamic CAI was used to plan and to place all implants following all the recommended digital steps. The software used provided a tool (Trace and Place) that made the merge between X-ray views of the residual teeth and their own positions possible. This method definitely registered that teeth positions comply with the required accuracy live check. After implants placement, a post-operative CBCT was taken in order to evaluate the deviations of the achieved implants at coronal, apical, and depth level as well as angular deviations. Statistically significant radiological mean difference between the two groups was found in the coronal position of implants (0.26 mm, p < 0.001), in the apical position of implants (0.29 mm, p < 0.001), in the depth of implants (0.16 mm, p = 0.022), and in the angular deviation (0.7, p = 0.004). The use of the TaP technology for the treatment of the patients with at least three stable teeth that need to be removed for a totally implant prosthetic treatment is a promising technique. The performed accuracy analysis demonstrated that this digital protocol can be used without a loss of accuracy of the achieved implants compared to planned ones.
The Influence of Racial Differences in the Demand for Healthcare in South Africa: A Case of Public Healthcare
Authors: Mhlanga David , Garidzirai Rufaro
Published: July 2020

The study sought to analyse the influence of racial differences in the demand for public healthcare in South Africa, using the 2018 General Household Survey (GHS) data. This was completed to understand if race still plays a role in access to healthcare in post-apartheid South Africa. Logistic regression analysis revealed that race significantly explained the variance in demand for public healthcare, with White populations having the lowest probability of demand for public healthcare compared to other races. Consequently, the study noted that racial differences still play a critical role in affording one access to healthcare after assessing the situation obtaining in public healthcare. Therefore, the study recommends that the government of South Africa should create policies that encourage equal access to basic services in addressing racial inequality in the country.
Healthy Behaviors through Behavioral Design–Obesity Prevention
Authors: Funderburk LesLee , Cardaci Thomas , Fink Andrew , Taylor Keyanna , Rohde Jane , Harris Debra
Published: July 2020

Evidence for behavior modification for improved health outcomes was evaluated for nutrition, physical activity (PA), and indoor environmental quality (IEQ). The databases searched included LISTA, PubMed, and Web of Science, with articles rated using an a priori baseline score of 70/100 to establish inclusion. The initial search produced 52,847 articles, 63 of which were included in the qualitative synthesis. Thirteen articles met inclusion for nutrition: cafeteria interventions, single interventions, and vending interventions. Seventeen articles on physical activity were included: stair use, walking, and adjustable desks. For IEQ, 33 articles met inclusion: circadian disruption, view and natural light, and artificial light. A narrative synthesis was used to find meaningful connections across interventions with evidence contributing to health improvements. Commonalities throughout the nutrition studies included choice architecture, increasing the availability of healthy food items, and point-of-purchase food labeling. Interventions that promoted PA included stair use, sit/stand furniture, workplace exercise facilities and walking. Exposure to natural light and views of natural elements were found to increase PA and improve sleep quality. Overexposure to artificial light may cause circadian disruption, suppressing melatonin and increasing risks of cancers. Overall, design that encourages healthy behaviors may lower risks associated with chronic disease.
Effects of Technology Use on Ageing in Place: The iZi Pilots
Authors: Silvius Helen A.M. , Tak Erwin C.P.M. , Mook-Kanamori Dennis O. , Vos Hedwig M.M. , Numans Mattijs E. , Chavannes Niels H.
Published: July 2020

In the iZi study in The Hague, use and acceptance of commercially available technology by home-dwelling older citizens was studied, by comparing self-efficacy and perceived physical and mental Quality of Life (QoL)-related parameters on an intervention location of 279 households and a control location of 301 households. Technology adoption was clinically significantly associated with increased perceived physical QoL, as compared with control group, depending on the number of technology interventions that were used. A higher number of adopted technologies was associated with a stronger effect on perceived QoL. We tried to establish a way to measure clinical significance by using mixed methods, combining quantitative and qualitative evaluation and feeding results and feedback of participants directly back into our intervention. In general, this research is promising, since it shows that successful and effective adoption of technology by older people is feasible with commercially available products amongst home-dwelling older citizens. We think this way of working provides a better integration of scientific methods and clinical usability but demands a lot of communication and patience of researchers, citizens, and policymakers. A change in policy on how to target people for this kind of intervention might be warranted.
Comprehensive Assessment of Water Quality and Pollution Source Apportionment in Wuliangsuhai Lake, Inner Mongolia, China
Authors: Shi Rui , Zhao Jixin , Shi Wei , Song Shuai , Wang Chenchen
Published: July 2020

Water quality is a key indicator of human health. Wuliangsuhai Lake plays an important role in maintaining the ecological balance of the region, protecting the local species diversity and maintaining agricultural development. However, it is also facing a greater risk of water quality deterioration. The 24 water quality factors that this study focused on were analyzed in water samples collected during the irrigation period and non-irrigation period from 19 different sites in Wuliangsuhai Lake, Inner Mongolia, China. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were conducted to evaluate complex water quality data and to explore the sources of pollution. The results showed that, during the irrigation period, sites in the middle part of the lake (clusters 1 and 3) had higher pollution levels due to receiving most of the agricultural and some industrial wastewater from the Hetao irrigation area. During the non-irrigation period, the distribution of the comprehensive pollution index was the opposite of that seen during the irrigation period, and the degree of pollutant index was reduced significantly. Thus, run-off from the Hetao irrigation area is likely to be the main source of pollution.
Impact of an Educational Program on Behavioral Changes toward Environmental Health among Laotian Students
Authors: Shin Jiyoung , Kim Harris Hyun-soo , Kim Eun Mee , Choi Yookyung , Ha Eunhee
Published: July 2020

This study evaluates the effect of an integrated health care educational program on several behavioral changes related to environmental health among Laotian students. Students in the experimental group received education concerning environmental health-related issues, including air pollution and chemical exposure. Analyses of covariance (ANCOVA) and paired t-tests were conducted for the statistical analysis of the pre- and post-survey scores. The post-test scores of the experimental group regarding their risk perception and information-seeking behaviors towards air pollution and chemical exposure were higher than those of the control group after controlling for the pre-test scores. Moreover, in the experimental group, the girls’ risk perception scores significantly increased after receiving the education, which was not observed in the control group. The risk perception score among non-drinking students also significantly increased after the program. These results indicate that the education program effectively enhanced the students’ risk perception, especially that of girls and nondelinquent students.
Food and Beverage Advertising Aimed at Spanish Children Issued through Mobile Devices: A Study from a Social Marketing and Happiness Management Perspective
Authors: Jiménez-Marín Gloria , Elías Zambrano Rodrigo , Galiano-Coronil Araceli , Ravina-Ripoll Rafael
Published: July 2020

Eating Disorders (ED) and obesity are a pandemic in developed and developing societies. In 2018, Spanish Ministry of Health and Consumption reported data on obesity (15%) and ED (12%). Spain thus ranks fifth among European countries in childhood obesity, with the highest incidence in the 6–12-year-age group. Many studies point to media as one of the contributing elements to this growth. In this sense, it should be noted that Spanish children are exposed to an average of 9000 television commercials per year and the vast majority of these are for food and beverage products of little or no nutritional value. Educommunication becomes essential here, since media have the capacity to educate, prevent and influence the behaviour as part of their social marketing strategies and within the happiness management philosophy. The aim of this paper is to analyse food and beverage advertising on mobile devices aimed at children. The methodology used includes a content analysis, a survey, and focus groups. The results show that many of the food products are bought or ordered as a direct result of advertising. The main conclusions point to the need to regulate the messages transmitted in order to guide the social function of media so that public health and happiness can be improved.
Health Impacts from Ambient Particle Exposure in Southern Sweden
Authors: Rittner Ralf , Flanagan Erin , Oudin Anna , Malmqvist Ebba
Published: July 2020

A health impact assessment (HIA) is an important tool for making informed decisions regarding the design and evaluation of environmental interventions. In this study, we performed a quantitative HIA for the population of Scania (1,247,993), the southernmost county in Sweden, in 2016. The impact of annual mean concentrations of particulate matter with an aerodynamic diameter <2.5 µm (PM2.5), modeled at their home residences for the year 2011, on mortality, asthma, dementia, autism spectrum disorders, preeclampsia and low birth weight (LBW) was explored. Concentration–response (C-R) functions were taken from epidemiological studies reporting meta-analyses when available, and otherwise from single epidemiological studies. The average level of PM2.5 experienced by the study population was 11.88 µg/m3. The PM2.5 exposure was estimated to cause 9–11% of cases of LBW and 6% of deaths from natural causes. Locally produced PM2.5 alone contributed to 2–9% of the cases of diseases and disorders investigated. Reducing concentrations to a maximum of 10 µg/m3 would, according to our estimations, reduce mortality by 3% and reduce cases of LBW by 2%. Further analyses of separate emission sources’ distinct effects were also presented. Reduction of air pollution levels in the study area would, as expected, have a substantial effect on both mortality and adverse health outcomes. Reductions should be aimed for by local authorities and on national and even international levels.
Spatiotemporal Evolution of Global Greenhouse Gas Emissions Transferring via Trade: Influencing Factors and Policy Implications
Authors: Zhong Zhangqi , Zhang Xu , Gao Weina
Published: July 2020

Global climate change caused by greenhouse gas emissions (GHGs) from anthropogenic activities have already become the focus of the world. A more systematic and comprehensive analysis on the factors influencing the changes of global GHGs transferring via trade have not been fully discussed. To this end, employing spatial econometric regression models and multi-regional input-output models, this paper reveals factors influencing the GHGs transferring via trade changes in 39 major economies, so as to develop the relevant GHGs reduction policies. The results indicate that regions with the highest net outflow of GHGs transferring via trade are primarily Russia and Canada, and the adverse effects of promoting GHGs reduction on the national economy could be avoided by these regions owing to trade relations. Additionally, factors influencing the changes in GHGs transferring via trade have significant spatial autocorrelation, and population size and energy structure exert significant spatial spillover effects on the changes in the GHGs transferring via trade. On this basis, this paper suggests that one more effective way to prevent trade from the rigorous demands of environmental governance measures while preserving the economic benefits of international trade may be to facilitate cooperation between countries on GHGs mitigation. Further, we articulate more balanced environment governance policies, including conducting the sharing of advanced energy technologies and developing clearer production technologies.
Reclaiming ʻĀina Health in Waimānalo
Authors: Keli‘iholokai LeShay , Keaulana Samantha , Antonio Mapuana C. K. , Rogerson Ikaika , Deitschman Kirk , Kamai Joseph Awa , Albinio Luana , Wilson Kilauea , Kepa Dawn , Makua Kuaiwi Laka , Vegas J. Kahaulahilahi , Chung-Do Jane J. , Ho Kenneth , Ho-Lastimosa H. Ilima
Published: July 2020

Kānaka Maoli (Native Hawaiian) worldviews of health emphasize pono (righteousness) and lōkahi (balance), which extends to include relationships with other people, akua (spiritual realm), and ʻāina (land). The purpose of this qualitative study was to explore the role of ʻāina and ʻāina connection in health and resilience based on the perspectives of 12 Kānaka Maoli adults from the Waimānalo community. Three major themes were identified: ʻĀina is everything, ʻāina is health, and community healing through community-led initiatives. A better understanding of ʻāina connection is important to improve our knowledge of Hawaiian health. A connection to ʻāina may specifically address health concerns resulting from historical trauma and environmental changes.
Benefits and Costs of a Community-Led Total Sanitation Intervention in Rural Ethiopia—A Trial-Based Ex Post Economic Evaluation
Authors: Cha Seungman , Jung Sunghoon , Belew Bizuneh Dawit , Abera Tadesse , Doh Young-Ah , Seong Jieun , Ross Ian
Published: July 2020

We estimated the costs and benefits of a community-led total sanitation (CLTS) intervention using the empirical results from a cluster-randomized controlled trial in rural Ethiopia. We modelled benefits and costs of the intervention over 10 years, as compared to an existing local government program. Health benefits were estimated as the value of averted mortality due to diarrheal disease and the cost of illness arising from averted diarrheal morbidity. We also estimated the value of time savings from avoided open defecation and use of neighbours’ latrines. Intervention delivery costs were estimated top-down based on financial records, while recurrent costs were estimated bottom-up from trial data. We explored methodological and parameter uncertainty using one-way and probabilistic sensitivity analyses. Avoided mortality accounted for 58% of total benefits, followed by time savings from increased access to household latrines. The base case benefit–cost ratio was 3.7 (95% CI: 1.9–5.4) and the net present value was Int’l $1,193,786 (95% CI: 406,017–1,977,960). The sources of the largest uncertainty in one-way sensitivity analyses were the effect of the CLTS intervention and the assumed lifespan of an improved latrine. Our results suggest that CLTS interventions can yield favourable economic returns, particularly if follow-up after the triggering is implemented intensively and uptake of improved latrines is achieved (as opposed to unimproved).
Subjective Overload and Psychological Distress among Dentists during COVID-19
Authors: Mijiritsky Eitan , Hamama-Raz Yaira , Liu Feng , Datarkar Abhay N. , Mangani Luca , Caplan Julian , Shacham Anna , Kolerman Roni , Mijiritsky Ori , Ben-Ezra Menachem , Shacham Maayan
Published: July 2020

Psychological distress during the COVID-19 pandemic is not solely limited to SARS-CoV-2 infection. It may also be related to social, cultural, and environmental factors, which may act as additional stressors. The aim of the current study was to explore the association between psychological distress and subjective overload among dentists in different countries, and whether it is associated with COVID-19-related factors. A cross-sectional survey was conducted among 1302 dentists from China, India, Israel, Italy, and the UK, who filled out demographics data, COVID-19-related factor questions, subjective overload, and psychological distress scales. Our findings showed that the positive association between subjective overload and psychological distress was different among countries, suggesting higher rate of intensity in Italy compared to China, India, and Israel (the UK was near significance with China and Israel). The interaction variable of the subjective overload × psychological distress was significantly associated with a particular country, with those individuals reporting fear of contracting COVID-19 from patients, fear of their families contracting COVID-19, and receiving enough professional knowledge regarding COVID-19. Given the above, dentists were found to have elevated levels of subjective overload and psychological distress, which differed among the countries, presumably due to certain background issues such as social, cultural, and environmental factors.
The Improved Health Outcomes Program (iHOP): A Unique Model to Promote Provider-Driven Research in a Medicaid Population
Authors: Schapmire Tara J. , Bell Jill , Pfeifer Mark P.
Published: July 2020

We describe an effort to improve the care of Medicaid and uninsured individuals through a three-way partnership between a Medicaid managed care insurer, front-line providers, and an academic university. The project provided annual funding over eleven years, for research, pilot programs, and demonstration projects. Projects were provider-driven in design and methods. The Medicaid-managed care insurer-funded proposals were vetted by a neutral university team experienced in grant writing and community-based research and scored by a community-based review panel. The grant program ran from 2007 to 2018, funding 41 projects, totaling USD 2,097,842. The partnership of an insurer, a university, and frontline providers was not only viable and sustainable for over a decade, but also flexible, free of project selection issues, and well-received by all stakeholders. Funded providers worked in both urban and rural settings and included hospitals, community non-profits, outpatient clinics, academic and community health partnerships, and public health agencies. The projects generally reflected common issues in the Medicaid and uninsured population needs, such as childhood obesity, and they were consistent with the targeted goals of the program. Broad health foci included child and/or maternal health, chronic conditions, mental health, preventive health, screening, system effectiveness, special populations including refugees, Latinos, and rural individuals, and substance use disorders. Details of the awarded grantee goals, the grants management process, and lessons learned from the partnership are presented. The partnership triad model was effective and stable, with each partner adding unique value. The use of the academic institution to administrate the program provided an arms-length relationship between the insurer and the providers in project selection and allowed assistance to less experienced researchers in community settings.
Social Capital and Preferences for Aging in Place Among Older Adults Living in Rural Northeast China
Authors: Lu Nan , Xu Shicun , Zhou Qinghong
Published: July 2020

The present study examines the association between cognitive social capital and structural social capital and aging-in-place among older adults living in rural Northeastern Chinese communities. Data were derived from a survey conducted in Dongliao County, Jilin Province, China, in late 2019. A quota sampling approach was used to recruit 458 respondents aged 60 years and older. Structural equation modeling was applied to test the proposed model. The results show that the measurement models of cognitive social capital and structural social capital were established in rural Chinese communities. Structural social capital was found to have a higher effect on aging in place than cognitive social capital (structural social capital: β = 0.241, p < 0.001; cognitive social capital: β = 0.118, p < 0.05). The findings highlight the crucial role both cognitive and structural social capital play in affecting the preference for aging in place among older rural Chinese adults. Policy and intervention implications are discussed.
A Generic and Extensible Core and Prototype of Consistent, Distributed, and Resilient LIS
Authors: Galić Zdravko , Vuzem Mario
Published: July 2020

The majority of the existing land information systems (LIS) are centralized, transaction processing systems based on object-relational database management systems for data storage, management, and retrieval. These traditional database management systems are dominantly based on a share-everything or share disk architecture and face challenges in meeting the performance and scalability requirements of distributed, data-intensive systems, including LIS. They support vertical, rather than horizontal scalability, which is of particular importance in distributed systems. In some cases, due to legal, administrative, or infrastructure constraints, LIS need to be distributed rather than centralized systems. Distributed computing systems and share-nothing architecture have become very popular, including new data processing platforms and frameworks with horizontal scalability and fault tolerance capabilities. In this paper, we present cdrLIS—a generic and extensible core of LIS based on relevant international standards and the NewSQL database management system (DBMS) that enables the implementation of consistent, distributed, highly-available, and resilient LIS. A generic core is implemented in the Go programming language and can be easily extended and adopted towards the implementation of a specific country profile. cdrLIS can be deployed either on a computer cluster or on cloud computing platforms and thus support the design and building of a new generation of distributed and resilient data-intensive applications and information systems in the land administration domain.
Interactive Web-Map of the European Freeway Junction A1/A4 Development with the Use of Archival Cartographic Sources
Authors: Lorek Dariusz , Horbiński Tymoteusz
Published: July 2020

In the article, authors have analyzed cartographic materials presenting the spatial development of Gliwice with the use of multimedia tools. The materials prove that this area has played an important part in the road system of the region, country and even part of Europe since the 19th century. The six maps from the studied area were analyzed e.g., the Urmesstischblätter map, polish topographic maps, and the OpenStreetMap. Based on these maps and their legends, vectorization of the main roads of the analyzed area was carried out. The evolution of the main road corridors on the six maps was analyzed with respect to the location of the European freeway junction (A1/A4), constituting a basis for the web map. According to the authors, the use of the interactive web map is the most comprehensive method of all technologies used by modern cartography. Spatial data collected from different cartographic publications (from the first half of the 19th century till the present) consider the most significant aspects of changes in the road network of the analyzed area in a detailed and user-friendly way.
Molecular Characterization of a Novel Lytic Enzyme LysC from Clostridium intestinale URNW and Its Antibacterial Activity Mediated by Positively Charged N-Terminal Extension
Authors: Plotka Magdalena , Szadkowska Monika , Håkansson Maria , Kovačič Rebeka , Al-Karadaghi Salam , Walse Björn , Werbowy Olesia , Kaczorowska Anna-Karina , Kaczorowski Tadeusz
Published: July 2020

Peptidoglycan hydrolytic enzymes are considered to be a promising alternative to conventional antibiotics in combating bacterial infections. To identify novel hydrolytic enzymes, we performed a database search with the sequences of two thermostable endolysins with high bactericidal activity, studied earlier in our laboratory. Both these enzymes originate from Thermus scotoductus bacteriophages MAT2119 and vB_Tsc2631. A lytic enzyme LysC from Clostridium intestinale URNW was found to have the highest amino acid sequence similarity to the bacteriophage proteins and was chosen for further analysis. The recombinant enzyme showed strong activity against its host bacteria C. intestinale, as well as against C. sporogenes, Bacillus cereus, Micrococcus luteus, and Staphylococcus aureus, on average causing a 5.12 ± 0.14 log reduction of viable S. aureus ATCC 25923 cells in a bactericidal assay. Crystallographic studies of the protein showed that the catalytic site of LysC contained a zinc atom coordinated by amino acid residues His50, His147, and Cys155, a feature characteristic for type 2 amidases. Surprisingly, neither of these residues, nor any other of the four conserved residues in the vicinity of the active site, His51, Thr52, Tyr76, and Thr153, were essential to maintain the antibacterial activity of LysC. Therefore, our attention was attracted to the intrinsically disordered and highly positively charged N-terminal region of the enzyme. Potential antibacterial activity of this part of the sequence, predicted by the Antimicrobial Sequence Scanning System, AMPA, was confirmed in our experimental studies; the truncated version of LysC (LysCΔ2–23) completely lacked antibacterial activity. Moreover, a synthetic peptide, which we termed Intestinalin, with a sequence identical to the first thirty amino acids of LysC, displayed substantial anti-staphylococcal activity with IC50 of 6 μg/mL (1.5 μM). This peptide was shown to have α-helical conformation in solution in the presence of detergents which is a common feature of amphipathic α-helical antimicrobial peptides.
Genome-Wide Identification and Expression Profile Analysis of the PHT1 Gene Family in Gossypium hirsutum and Its Two Close Relatives of Subgenome Donor Species
Authors: Cai Sheng , Liu Fujie , Zhou Baoliang
Published: July 2020

Phosphate transporter (PHT) is responsible for plant phosphorus (P) absorption and transport. PHT1 is a component of the high-affinity phosphate transporter system and plays pivotal roles in P absorption under P starvation conditions. However, in cotton, the number and identity of PHT1 genes that are crucial for P absorption from soil remain unclear. Here, genome-wide identification detected twelve PHT1 genes in Gossypium hirsutum and seven and eight PHT1 genes in two close relatives of the G. hirsutum genome—G. arboreum and G. raimondii, respectively. In addition, under low-phosphate treatment, the expressions of GaPHT1;3, GaPHT1;4, and GaPHT1;5 in roots were upregulated after 3 h of induction, and GhPHT1;3-At, GhPHT1;4-At, GhPHT1;5-At, GhPHT1;3-Dt, GhPHT1;4-Dt, and GhPHT1;5-Dt in the roots began to respond after 1 h of induction. Homologous pairs—GaPHT1;4 and GhPHT1;4-At; GaPHT1;5 and GhPHT1;5-At; GrPHT1;4 and GhPHT1;4-Dt, with GhPHT1;5-Dt and GhPHT1;5-At being syntenic—were all highly expressed in the roots under normal conditions. Among the genes highly expressed in the roots, GhPHT1;4-At, GhPHT1;5-At, GhPHT1;4-Dt and GhPHT1;5-Dt were continuously upregulated by P starvation. Therefore, it is concluded that these four genes might be key genes for P uptake in cotton roots. The results of this study provide insights into the mechanisms of P absorption and transport in cotton.
Genetic Polymorphisms Associated with Rheumatoid Arthritis Development and Antirheumatic Therapy Response
Authors: Mikhaylenko Dmitry S. , Nemtsova Marina V. , Bure Irina V. , Kuznetsova Ekaterina B. , Alekseeva Ekaterina A. , Tarasov Vadim V. , Lukashev Alexander N. , Beloukhova Marina I. , Deviatkin Andrei A. , Zamyatnin Andrey A.
Published: July 2020

Rheumatoid arthritis (RA) is the most common inflammatory arthropathy worldwide. Possible manifestations of RA can be represented by a wide variability of symptoms, clinical forms, and course options. This multifactorial disease is triggered by a genetic predisposition and environmental factors. Both clinical and genealogical studies have demonstrated disease case accumulation in families. Revealing the impact of candidate gene missense variants on the disease course elucidates understanding of RA molecular pathogenesis. A multivariate genomewide association study (GWAS) based analysis identified the genes and signalling pathways involved in the pathogenesis of the disease. However, these identified RA candidate gene variants only explain 30% of familial disease cases. The genetic causes for a significant proportion of familial RA have not been determined until now. Therefore, it is important to identify RA risk groups in different populations, as well as the possible prognostic value of some genetic variants for disease development, progression, and treatment. Our review has two purposes. First, to summarise the data on RA candidate genes and the increased disease risk associated with these alleles in various populations. Second, to describe how the genetic variants can be used in the selection of drugs for the treatment of RA.
Thrombospondin-1 Plays an Essential Role in Yes-Associated Protein Nuclear Translocation during the Early Phase of Trypanosoma cruzi Infection in Heart Endothelial Cells
Authors: Arun Ashutosh , Rayford Kayla J. , Cooley Ayorinde , Rachakonda Girish , Villalta Fernando , Pratap Siddharth , Lima Maria F. , Sheibani Nader , Nde Pius N.
Published: July 2020

The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease. This neglected tropical disease causes severe morbidity and mortality in endemic regions. About 30% of T. cruzi infected individuals will present with cardiac complications. Invasive trypomastigotes released from infected cells can be carried in the vascular endothelial system to infect neighboring and distant cells. During the process of cellular infection, the parasite induces host cells, to increase the levels of host thrombospondin-1 (TSP-1), to facilitate the process of infection. TSP-1 plays important roles in the functioning of vascular cells, including vascular endothelial cells with important implications in cardiovascular health. Many signal transduction pathways, including the yes-associated protein 1 (YAP)/transcriptional coactivator, with PDZ-binding motif (TAZ) signaling, which are upstream of TSP-1, have been linked to the pathophysiology of heart damage. The molecular mechanisms by which T. cruzi signals, and eventually infects, heart endothelial cells remain unknown. To evaluate the importance of TSP-1 expression in heart endothelial cells during the process of T. cruzi infection, we exposed heart endothelial cells prepared from Wild Type and TSP-1 Knockout mouse to invasive T. cruzi trypomastigotes at multiple time points, and evaluated changes in the hippo signaling cascade using immunoblotting and immunofluorescence assays. We found that the parasite turned off the hippo signaling pathway in TSP-1KO heart endothelial cells. The levels of SAV1 and MOB1A increased to a maximum of 2.70 ± 0.23 and 5.74 ± 1.45-fold at 3 and 6 h, respectively, in TSP-1KO mouse heart endothelial cells (MHEC), compared to WT MHEC, following a parasite challenge. This was accompanied by a significant continuous increase in the nuclear translocation of downstream effector molecule YAP, to a maximum mean nuclear fluorescence intensity of 10.14 ± 0.40 at 6 h, compared to wild type cells. Furthermore, we found that increased nuclear translocated YAP significantly colocalized with the transcription co-activator molecule pan-TEAD, with a maximum Pearson’s correlation coefficient of 0.51 ± 0.06 at 6 h, compared to YAP-Pan-TEAD colocalization in the WT MHEC, which decreased significantly, with a minimum Pearson’s correlation coefficient of 0.30 ± 0.01 at 6 h. Our data indicate that, during the early phase of infection, upregulated TSP-1 is essential for the regulation of the hippo signaling pathway. These studies advance our understanding of the molecular interactions occurring between heart endothelial cells and T. cruzi, in the presence and absence of TSP-1, providing insights into processes linked to parasite dissemination and pathogenesis.
Targeting Wnt/β-Catenin Pathway for Developing Therapies for Hair Loss
Authors: Choi Bu Young
Published: July 2020

Persistent hair loss is a major cause of psychological distress and compromised quality of life in millions of people worldwide. Remarkable progress has been made in understanding the molecular basis of hair loss and identifying valid intracellular targets for designing effective therapies for hair loss treatment. Whereas a variety of growth factors and signaling pathways have been implicated in hair cycling process, the activation of Wnt/β-catenin signaling plays a central role in hair follicle regeneration. Several plant-derived chemicals have been reported to promote hair growth by activating Wnt/β-catenin signaling in various in vitro and in vivo studies. This mini-review sheds light on the role of Wnt/β-catenin in promoting hair growth and the current progress in designing hair loss therapies by targeting this signaling pathway.
Mice Lacking Connective Tissue Growth Factor in the Forebrain Exhibit Delayed Seizure Response, Reduced C-Fos Expression and Different Microglial Phenotype Following Acute PTZ Injection
Authors: Siow Pei-Fen , Tsao Chih-Yu , Chang Ho-Ching , Chen Chwen-Yu , Yu I-Shing , Lee Kuang-Yung , Lee Li-Jen
Published: July 2020

Connective tissue growth factor (CTGF) plays important roles in the development and regeneration of the connective tissue, yet its function in the nervous system is still not clear. CTGF is expressed in some distinct regions of the brain, including the dorsal endopiriform nucleus (DEPN) which has been recognized as an epileptogenic zone. We generated a forebrain-specific Ctgf knockout (FbCtgf KO) mouse line in which the expression of Ctgf in the DEPN is eliminated. In this study, we adopted a pentylenetetrazole (PTZ)-induced seizure model and found similar severity and latencies to death between FbCtgf KO and WT mice. Interestingly, there was a delay in the seizure reactions in the mutant mice. We further observed reduced c-fos expression subsequent to PTZ treatment in the KO mice, especially in the hippocampus. While the densities of astrocytes and microglia in the hippocampus were kept constant after acute PTZ treatment, microglial morphology was different between genotypes. Our present study demonstrated that in the FbCtgf KO mice, PTZ failed to increase neuronal activity and microglial response in the hippocampus. Our results suggested that inhibition of Ctgf function may have a therapeutic potential in preventing the pathophysiology of epilepsy.
Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC
Authors: Zito Marino Federica , Botti Gerardo , Aquino Gabriella , Ferrero Stefano , Gaudioso Gabriella , Palleschi Alessandro , Rocco Danilo , Salvi Rosario , Micheli Maria Carolina , Micheli Pietro , Morabito Alessandro , Rocco Gaetano , Giordano Antonio , De Cecio Rossella , Franco Renato
Published: July 2020

Background: The Anaplastic Lymphoma Kinase (ALK) gene is known to be affected by several genetic alterations, such as rearrangement, amplification and point mutation. The main goal of this study was to comprehensively analyze ALK amplification (ALK-A) and ALK gene copy number gain (ALK-CNG) in a large cohort of non-small-cell lung cancer (NSCLC) patients in order to evaluate the effects on mRNA and protein expression. Methods: ALK locus number status was evaluated in 578 NSCLC cases by fluorescence in situ hybridization (FISH). In addition, ALK immunohistochemistry and ALK mRNA in situ hybridization were performed. Results: Out of 578 cases, 17 cases showed ALK-A. In addition, 14 cases presented ALK-CNG and 72 cases presented chromosome 2 polyploidy. None of those carrying ALK-A and -CNG showed either ALK immunohistochemical expression or ALK mRNA expression through in situ hybridization. We observed a high frequency of extra copies of the ALK gene. Conclusions: Our findings demonstrated that ALK-A is not involved in mRNA production and consequently is not involved in protein production; these findings support the hypothesis that ALK-A might not play a role in the pathogenesis of NSCLC, underlining the absence of a specific clinical application.
Genome-Wide Association Study Unravels LRK1 as a Dark Respiration Regulator in Rice (Oryza sativa L.)
Authors: Qu Mingnan , Essemine Jemaa , Li Ming , Chang Shuoqi , Chang Tiangen , Chen Gen-Yun , Zhu Xin-Guang
Published: July 2020

Respiration is a major plant physiological process that generates adenosine triphosphate (ATP) to support the various pathways involved in the plant growth and development. After decades of focused research on basic mechanisms of respiration, the processes and major proteins involved in respiration are well elucidated. However, much less is known about the natural variation of respiration. Here we conducted a survey on the natural variation of leaf dark respiration (Rd) in a global rice minicore diversity panel and applied a genome-wide association study (GWAS) in rice (Oryza sativa L.) to determine candidate loci associated with Rd. This rice minicore diversity panel consists of 206 accessions, which were grown under both growth room (GR) and field conditions. We found that Rd shows high single-nucleotide polymorphism (SNP) heritability under GR and it is significantly affected by genotype-environment interactions. Rd also exhibits strong positive correlation to the leaf thickness and chlorophyll content. GWAS results of Rd collected under GR and field show an overlapped genomic region in the chromosome 3 (Chr.3), which contains a lead SNP (3m29440628). There are 12 candidate genes within this region; among them, three genes show significantly higher expression levels in accessions with high Rd. Particularly, we observed that the LRK1 gene, annotated as leucine rich repeat receptor kinase, was up-regulated four times. We further found that a single significantly associated SNPs at the promoter region of LRK1, was strongly correlated with the mean annual temperature of the regions from where minicore accessions were collected. A rice lrk1 mutant shows only ~37% Rd of that of WT and retarded growth following exposure to 35 °C for 30 days, but only 24% reduction in growth was recorded under normal temperature (25 °C). This study demonstrates a substantial natural variation of Rd in rice and that the LRK1 gene can regulate leaf dark respiratory fluxes, especially under high temperature.
P2X7 Receptor at the Crossroads of T Cell Fate
Authors: Rivas-Yáñez Elizabeth , Barrera-Avalos Carlos , Parra-Tello Brian , Briceño Pedro , Rosemblatt Mariana V. , Saavedra-Almarza Juan , Rosemblatt Mario , Acuña-Castillo Claudio , Bono María Rosa , Sauma Daniela
Published: July 2020

The P2X7 receptor is a ligand-gated, cation-selective channel whose main physiological ligand is ATP. P2X7 receptor activation may also be triggered by ARTC2.2-dependent ADP ribosylation in the presence of extracellular NAD. Upon activation, this receptor induces several responses, including the influx of calcium and sodium ions, phosphatidylserine externalization, the formation of a non-selective membrane pore, and ultimately cell death. P2X7 receptor activation depends on the availability of extracellular nucleotides, whose concentrations are regulated by the action of extracellular nucleotidases such as CD39 and CD38. The P2X7 receptor has been extensively studied in the context of the immune response, and it has been reported to be involved in inflammasome activation, cytokine production, and the migration of different innate immune cells in response to ATP. In adaptive immune responses, the P2X7 receptor has been linked to T cell activation, differentiation, and apoptosis induction. In this review, we will discuss the evidence of the role of the P2X7 receptor on T cell differentiation and in the control of T cell responses in inflammatory conditions.
Biopolymers as Food Packaging Materials
Authors: Porta Raffaele , Sabbah Mohammed , Di Pierro Prospero
Published: July 2020

Oil-derived plastics are the most commonly used materials for packaging because of their features, low cost, and availability of resources for manufacturing [...]
Crosstalk of Brain and Bone—Clinical Observations and Their Molecular Bases
Authors: Otto Ellen , Knapstein Paul-Richard , Jahn Denise , Appelt Jessika , Frosch Karl-Heinz , Tsitsilonis Serafeim , Keller Johannes
Published: July 2020

As brain and bone disorders represent major health issues worldwide, substantial clinical investigations demonstrated a bidirectional crosstalk on several levels, mechanistically linking both apparently unrelated organs. While multiple stress, mood and neurodegenerative brain disorders are associated with osteoporosis, rare genetic skeletal diseases display impaired brain development and function. Along with brain and bone pathologies, particularly trauma events highlight the strong interaction of both organs. This review summarizes clinical and experimental observations reported for the crosstalk of brain and bone, followed by a detailed overview of their molecular bases. While brain-derived molecules affecting bone include central regulators, transmitters of the sympathetic, parasympathetic and sensory nervous system, bone-derived mediators altering brain function are released from bone cells and the bone marrow. Although the main pathways of the brain-bone crosstalk remain ‘efferent’, signaling from brain to bone, this review emphasizes the emergence of bone as a crucial ‘afferent’ regulator of cerebral development, function and pathophysiology. Therefore, unraveling the physiological and pathological bases of brain-bone interactions revealed promising pharmacologic targets and novel treatment strategies promoting concurrent brain and bone recovery.