bluuebunny/crossref_metadata_2025 huggingface dataset:
Carbon Input and Maize Productivity as Influenced by Tillage, Crop Rotation, Residue Management and Biochar in a Semiarid Region in South Africa
Authors: Nyambo Patrick , Chiduza Cornelius , Araya Tesfay
Published: May 2020

Conservation agriculture (CA) as a system is still evolving on many of the smallholder farms in sub-Saharan Africa (SSA) and questions on the impact of individual components and pathways toward adoption still require answers. A short-term study was conducted to investigate the effect of tillage, crop rotation, and crop residue management, including maize residue biochar on above ground biomass, cumulative carbon (C) input, soil organic carbon (SOC), and maize grain yield. A split–split plot design was used to evaluate two tillage operations (conventional tillage (CT) and no-till (NT)), three crop rotations (maize–fallow–maize (MFM), maize–oat–maize (MOM), and maize–vetch–maize (MVM)), and three-crop residue management (retention (R+), removal (R−), and biochar (B)). The cumulative above ground biomass produced in the MOM rotation was significantly higher by 78.9% and 88.7% relative to MVM and MFM rotations, respectively. The cumulative C input under residue management treatments ranged from 10.65 to 12.16 Mg ha−1. The highest SOC was observed under R+ (1.10%) followed by B (1.0%) and the lowest was in R− (0.96%). Crop residue management significantly affected grain yields in 2015/2016 (p < 0.05) and 2016/2017 (p < 0.01) summer seasons. Biochar did not result in an obvious improvement in both C input and crop yield. Smallholder farmers can potentially switch from CT to NT without any significant yield penalty, as well as adopt MOM and R+ practices for increased biomass and C input.
Assessing the Ability of Durum Wheat-Thinopyrum ponticum Recombinant Lines to Suppress Naturally Occurring Weeds under Different Sowing Densities
Authors: Rossini Francesco , Provenzano Maria Elena , Kuzmanović Ljiljana , Ceoloni Carla , Ruggeri Roberto
Published: May 2020

The use of synthetic chemicals in cropping systems is becoming more controversial and highly debated worldwide, owing to its impacts on the environment, food safety, and human health. For this reason, sustainable crop management strategies are gaining increasing interest. In this perspective, agronomic practices and use of disease-resistant and competitive genotypes represent valuable tools in the hands of farmers. The competitive ability of two durum wheat-Thinopyrum ponticum recombinant lines (named R5 and R112), carrying effective resistance genes towards main rust diseases and enhanced yield-related traits in their alien chromosome segments, was investigated in comparison with that of a widely grown commercial cultivar (Tirex), under two sowing densities (250 and 350 seeds m−2), in the presence or absence of weeds. Yield-related traits and specific attributes that confer competitive ability were recorded in two subsequent seasons. R5 was the most weed-suppressive genotype, whereas Tirex was the least competitive. R112 was the best yield performer under favorable weather conditions (5.6 t ha−1), while it suffered the presence of weeds in the drier year (−38% grain yield). Although 350 seeds m−2 appeared to be the most effective sowing density for suppression of weeds (−16% weed biomass), adoption of the lower density can optimize grain yield and limit weed infestation in dry seasons. A suitable combination of sowing density and genotype choice can improve yield performance. R112 required the higher sowing rate to maximize grain yield (+43% as compared to the lower sowing rate), while R5 proved to be a density-neutral genotype.
Mapping Productivity and Essential Biophysical Parameters of Cultivated Tropical Grasslands from Sentinel-2 Imagery
Authors: Cisneros Amparo , Fiorio Peterson , Menezes Patricia , Pasqualotto Nieves , Van Wittenberghe Shari , Bayma Gustavo , Furlan Nogueira Sandra
Published: May 2020

Nitrogen (N) is the main nutrient element that maintains productivity in forages; it is inextricably linked to dry matter increase and plant support capacity. In recent years, high spectral and spatial resolution remote sensors, e.g., the European Space Agency (ESA)’s Sentinel satellite missions, have become freely available for agricultural science, and have proven to be powerful monitoring tools. The use of vegetation indices has been essential for crop monitoring and biomass estimation models. The objective of this work is to test and demonstrate the applicability of different vegetation indices to estimate the biomass productivity, the foliar nitrogen content (FNC), the plant height and the leaf area index (LAI) of several tropical grasslands species submitted to different nitrogen (N) rates in an experimental area of São Paulo, Brazil. Field reflectance data of Panicum maximum and Urochloa brizantha species’ cultivars were taken and convoluted to the Sentinel-2 satellite bands. Subsequently, different vegetation indices (Normalized Difference Vegetation Index (NDI), Three Band Index (TBI), Difference light Height (DLH), Three Band Dall’Olmo (DO), and Normalized Area Over reflectance Curve (NAOC)) were tested for the experimental grassland areas, and composed of Urochloa decumbens and Urochloa brizantha grass species, which were sampled and destructively analyzed. Our results show the use of different relevant Sentinel-2 bands in the visible (VIS)–near infrared (NIR) regions for the estimation of the different biophysical parameters. The FNC obtained the best correlation for the TBI index combining blue, green and red bands with a determination coefficient (R2) of 0.38 and Root Mean Square Error (RMSE) of 3.4 g kg−1. The estimation of grassland productivity based on red-edge and NIR bands showed a R2 = 0.54 and a RMSE = 1800 kg ha−1. For the LAI, the best index was the NAOC (R2 = 0.57 and RMSE = 1.4 m2 m−2). High values of FNC, productivity and LAI based on different sets of Sentinel-2 bands were consistently obtained for areas under N fertilization.
Effect of Chinese Milk Vetch (Astragalus sinicus L.) and Rice Straw Incorporated in Paddy Soil on Greenhouse Gas Emission and Soil Properties
Authors: Ma Qiaoying , Li Jiwei , Aamer Muhammad , Huang Guoqin
Published: May 2020

Paddy soil is an important emission source of agricultural greenhouse gases. The excessive application of chemical fertilizer to paddy soil is one of the important reasons for high greenhouse gas emissions. Emissions can be reduced through optimized agricultural management measures. The incorporation of Chinese milk vetch (CMV) and rice straw in the field to replace some of the chemical fertilizer can reduce the emissions of greenhouse gases, but the relationship between these emissions and soil properties after the incorporation of CMV and rice straw is unclear. Through the continuous determination of greenhouse gases and the physical and chemical properties of soil, it was found that the addition of CMV and straw could increase the emissions of methane (CH4) and carbon dioxide (CO2), but nitrous oxide (N2O) emissions were lower. The effect of the combined incorporating of CMV and rice straw on soil properties was more significant than CMV alone. It was also found that CH4 and CO2 emissions were positively correlated with microbial biomass carbon and nitrogen, pH, and soil catalase and β-xylosidase activities. In practice, we can reduce greenhouse gas emissions by water and fertilizer management.
Dolomite and Compost Amendments Enhance Cu Phytostabilization and Increase Microbiota of the Leachates from a Cu-Contaminated Soil
Authors: Giagnoni Laura , dos Anjos Borges Luiz Gustavo , Giongo Adriana , de Oliveira Silveira Andressa , Ardissone Alexandria N. , Triplett Eric W. , Mench Michel , Renella Giancarlo
Published: May 2020

The chemical properties, ecotoxicity, and microbiome of leachates from phytomanaged Cu-contaminated soils were analyzed. The phytomanagement was carried out using Cu-tolerant poplar Populus trichocarpa × deltoides cv. Beaupré and black bent Agrostis gigantea L., aided by soil amendments, i.e., dolomitic limestone (DL) and compost (OM), alone and in combination (OMDL). Plants plus either DL or OMDL amendments reduced in leachates the electrical conductivity, the Cu concentration, and the concentration of total organic C except for the OMDL treatment, and decreased leachate toxicity towards bacteria. Total N concentration increased in the OM leachates. The aided phytostabilization increased the culturable bacteria numbers and the proportion of Cu-resistant bacteria in the leachates, as compared to the leachate from the untreated soil. Phytomanagement also enriched the microbial communities of the leachates with plant beneficial bacteria. Overall, the Cu stabilization and phytomanagement induced positive changes in the microbial communities of the soil leachates.
Study of Wine Producers’ Marketing Communication in Extreme Territories–Application of the AGIL Scheme to Wineries’ Website Features
Authors: Chironi Stefania , Altamore Luca , Columba Pietro , Bacarella Simona , Ingrassia Marzia
Published: May 2020

This study measures the communication ability of wineries in two extreme territories of Southern Italy, Mount Etna and the island of Pantelleria. The evaluation of four dimensions of web communication was carried out by the AGIL Scheme (i.e., adaptation, goal-attainment, integration, latent pattern maintenance). The study provides a generalizable model to be applied in other similar studies. Additionally, focus groups of experts were carried out. The method proved to be suitable to measure the communication effectiveness of wineries through websites. Extreme territories may add value to the wine, regardless of the brand. The heroic wines may become the symbol of these territories, helping environmentally safeguard and contrast territory abandonment by rural communities. The findings highlight that effective communication of heroic viticulture may be used to reposition these wines and increase their competitive advantage in foreign markets. The study generates new ideas for reflection on new types of web communication.
Changes in Agronomic and Physiological Traits of Sugarcane Grown with Saline Irrigation Water
Authors: Watanabe Kenta , Takaragawa Hiroo , Ueno Masami , Kawamitsu Yoshinobu
Published: May 2020

In Japan, the highest salt concentration in irrigation water for sugarcane cultivation has been reported to be above 2500 mg L−1, which may cause harmful effects to the crops; however, little information is available on the relationship between the salinity of irrigation water and sugarcane. To investigate its effects on agronomic and physiological traits, a Japanese cultivar, Saccharum spp cv. NiF8, was grown with 0, 200, 500, 1000, 2000, and 3000 mg NaCl L−1 under pot conditions. The treatments significantly lowered leaf area; however, NaCl levels up to 500 mg L−1 did not greatly reduce culm weight and juice sugar concentration. These traits were impaired when the tested cultivar was grown with 1000 mg NaCl L−1 or higher, indicating that salt concentration is desired to be lower than 1000 mg L−1. CO2 assimilation rate was inhibited mainly due to stomatal closure caused by salt stress. The treatments significantly altered Na+, Cl−, and K+ concentrations in juice but not those in leaf, suggesting that juice analysis is an effective method to estimate its salinization status. Culm weight and juice sugar concentration were severely affected as juice conductivity exceeded 900 mS m−1; thereby, sugarcane plants of NiF8 possessing conductivity above this level could be considered salt-stressed where water salinity is a concern.
Egg Quality from Siciliana and Livorno Italian Autochthonous Chicken Breeds Reared in Organic System
Authors: Di Rosa Ambra R. , Chiofalo Biagina , Lo Presti Vittorio , Chiofalo Vincenzo , Liotta Luigi
Published: May 2020

In poultry production, the intensive use of high-performing hybrid animals led to loss of genetic variability and a consequent lower response to climatic change and disease. Poultry biodiversity is seriously threatened, and its safeguard is a strong objective in developed countries. According to the FAO, which emphasized the importance of native breeds for its country of origin, the aim of this study was to present the first contribution on eggs quality for endangered the Siciliana chicken breed and deepen knowledge on the local Livorno breed. At 20 weeks of age, 108 laying hens (54 Siciliana breed and 54 Livorno breed) were divided into six homogeneous groups of 18 hens each and reared according to requirements imposed by the EC Regulation 889/08 for organic production. The production cycle was controlled over one year, and egg production was recorded daily by group. Eggs were collected, weighted, and measured. Physico-chemical parameter and fatty acids profile were analyzed and nutritional indexes calculated. The statistical model included the effects of breed (Siciliana, Livorno). Egg production was 190 egg/head for Siciliana and 180 for Livorno group. The results showed similar values for Siciliana and Livorno egg quality, highlighting several valuable quality traits from these breeds which might be taken into account for conservation programs.
Resilience of Small Ruminants to Climate Change and Increased Environmental Temperature: A Review
Authors: Joy Aleena , Dunshea Frank R. , Leury Brian J. , Clarke Iain J. , DiGiacomo Kristy , Chauhan Surinder S.
Published: May 2020

Climate change is a major global threat to the sustainability of livestock systems. Climatic factors such as ambient temperature, relative humidity, direct and indirect solar radiation and wind speed influence feed and water availability, fodder quality and disease occurrence, with production being most efficient in optimal environmental conditions. Among these climatic variables, ambient temperature fluctuations have the most impact on livestock production and animal welfare. Continuous exposure of the animals to heat stress compromises growth, milk and meat production and reproduction. The capacity of an animal to mitigate effects of increased environmental temperature, without progressing into stress response, differs within and between species. Comparatively, small ruminants are better adapted to hot environments than large ruminants and have better ability to survive, produce and reproduce in harsh climatic regions. Nevertheless, the physiological and behavioral changes in response to hot environments affect small ruminant production. It has been found that tropical breeds are more adaptive to hot climates than high-producing temperate breeds. The growing body of knowledge on the negative impact of heat stress on small ruminant production and welfare will assist in the development of suitable strategies to mitigate heat stress. Selection of thermotolerant breeds, through identification of genetic traits for adaption to extreme environmental conditions (high temperature, feed scarcity, water scarcity), is a viable strategy to combat climate change and minimize the impact on small ruminant production and welfare. This review highlights such adaption within and among different breeds of small ruminants challenged by heat stress.
The Use of Bacteriophages in the Poultry Industry
Authors: Żbikowska Katarzyna , Michalczuk Monika , Dolka Beata
Published: May 2020

The emergence of multidrug-resistant infections and antibiotic failures have raised concerns over human and veterinary medicine worldwide. Poultry production has had to confront the problems of an alarming increase in bacterial resistance, including zoonotic pathogens. According to the European Food Safety Authority (EFSA), campylobacteriosis and salmonellosis have been the most frequently reported human foodborne diseases linked to poultry. This situation has strongly stimulated a renewal of scientists’ interest in bacteriophages (phages) since the beginning of the 21st century. Bacteriophages are the viruses of bacteria. They are abundant in nature, and accompany bacteria in each environment they colonize, including human microbiota. In this review, we focused on the use of bacteriophages as therapeutic agents to treat infections and reduce counts of pathogenic bacteria in poultry, as biocontrol agents to eliminate foodborne pathogens on/in food, and also as disinfectants to reduce contamination on food-contact surfaces or poultry carcasses in industrial conditions. Most of the phage-based products are targeted against the main foodborne pathogens, such as Campylobacter jejuni, Salmonella spp., Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Clostridium perfringens. Phages are currently addressed at all stages of the poultry production "from farm to fork", however, their implementation into live birds and food products still provokes discussions especially in the context of the current legal framework, limitations, as well as public health and safety.
Can We Observe Expected Behaviors at Large and Individual Scales for Feed Efficiency-Related Traits Predicted Partly from Milk Mid-Infrared Spectra?
Authors: Zhang Lei , Gengler Nicolas , Dehareng Frédéric , Colinet Frédéric , Froidmont Eric , Soyeurt Hélène
Published: May 2020

Phenotypes related to feed efficiency were predicted from records easily acquired by breeding organizations. A total of 461,036 and 354,148 records were collected from the first and second parity Holstein cows. Equations were applied to the milk mid-infrared spectra to predict the main milk components and coupled with animal characteristics to predict the body weight (pBW). Dry matter intake (pDMI) was predicted from pBW using the National Research Council (NRC) equation. The consumption index (pIC) was estimated from pDMI and fat, and protein corrected milk. All traits were modeled using single trait test-day models. Descriptive statistics were within the expected range. Milk yield, pDMI, and pBW were phenotypically positively related (r ranged from 0.08 to 0.64). As expected, pIC was phenotypically negatively correlated with milk yield (−0.77 and −0.80 for the first and second lactation) and slightly positively correlated with pBW (0.16 and 0.07 for the first and second lactation). Later, parity cows seemed to have a better feed efficiency as they had a lower pIC. Although the prediction accuracy was moderate, the observed behaviors of studied traits by year, stage of lactation, and parity were in agreement with the literature. Moreover, as a genetic component was highlighted (heritability around 0.18), it would be interesting to realize a genetic evaluation of these traits and compare the obtained breeding values with the ones estimated for sires having daughters with reference feed efficiency records.
Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus
Authors: Gilannejad Neda , de las Heras Verónica , Martos-Sitcha Juan Antonio , Moyano Francisco J. , Yúfera Manuel , Martínez-Rodríguez Gonzalo
Published: May 2020

Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of key digestive enzymes was measured from three until 90 days post hatch (dph). Expression of genes involved in digestion of proteins (try2, ctr, pga2, and atp4a), carbohydrates (amy2a), and lipids (cel and pla2g1b), together with two somatotropic factors (gh and igf1) were also quantified. No chymotrypsin or pepsin activities were detected. While specific activity of trypsin and lipase were high during the first 30 dph and declined afterward, amylase activity was low until 57 dph and increased significantly beyond that point. Expression of try2, ctr, amy2a, and cel increased continuously along development, and showed a peak at the end of metamorphosis. Expression of pla2g1b, pga2 and atp4a increased until the middle of metamorphosis and decreased afterwars. Most of these trends contrast the usual patterns in carnivorous species and highlight the transition from larvae, with high protein requirements, to post-larvae/juvenile stages, with omnivorous/detritivorous feeding preferences. Somatotropic genes, gh and igf1, showed approximately inverse expression patterns, suggesting the establishment of the Gh/Igf1 axis from 50 dph.
Can Isoflurane and Meloxicam Mitigate Pain Associated with Cautery Disbudding of 3-Week-Old Goat Kids?
Authors: Hempstead Melissa N. , Waas Joseph R. , Stewart Mairi , Cave Vanessa M. , Sutherland Mhairi A.
Published: May 2020

We evaluated the effect of pain mitigation strategies (isoflurane and meloxicam) on the behaviour and physiology of 3-week-old disbudded goat kids. Fifty Saanen does (mean ± SD, 21 ± 3 days old) were randomly allocated to one of five treatments: (1) cautery-disbudded (CAUT), (2) CAUT + isoflurane (ISO), (3) CAUT + isoflurane + meloxicam (ISO + MEL), (4) CAUT + meloxicam (MEL), and (5) handled without disbudding or pain relief (SHAM). Blood samples were taken immediately prior to treatment and at 15-, 60- and 120-min post-treatment to assess cortisol, glucose and lactate concentrations. Behaviour (head shaking and scratching, body shaking, feeding and self-grooming) was observed for 1 h pre- and post-treatment using video-cameras. ISO + MEL and ISO kids had lower cortisol concentrations than CAUT kids 15 min post-treatment (p ≤ 0.05). There was no effect of treatment or time for glucose and lactate concentrations (p ≥ 0.62). At 35 min post-treatment, CAUT, MEL and ISO kids performed more head shakes than SHAM kids (p ≤ 0.05). Isoflurane, with or without meloxicam, may reduce acute stress associated with disbudding of 3-week-old goat kids. More research is needed to assess whether isoflurane (with or without meloxicam) can provide sufficient pain relief for disbudding 3-week-old kids.
A Purification Strategy Utilizing Hydrophobic Interaction Chromatography to Obtain Homogeneous Species from a Site-Specific Antibody Drug Conjugate Produced by AJICAP™ First Generation
Authors: Matsuda Yutaka , Leung Monica , Okuzumi Tatsuya , Mendelsohn Brian
Published: May 2020

In recent years, site-specific antibody drug conjugates (ADC)s have been in great demand because they have an expanded therapeutic index compared with conventional ADCs. AJICAP™ technology is a chemical conjugation platform to obtain site-specific ADCs through the use of a class of Fc-affinity compounds. Promising results from early technology development studies led to further investigation of AJICAP™ ADC materials to obtain site-specific and homogeneous drug antibody ratio (DAR) ADCs. Here we report site-specific conjugation followed by a preparative hydrophobic interaction chromatography (HIC) purification strategy to obtain purified “DAR = 1.0” and “DAR = 2.0” AJICAP™ ADC materials. Optimization of the mobile phase conditions and resin achieved a high recovery rate. In vitro biological assay demonstrated the target selective activity for purified homogeneous DAR ADCs. These results indicate the ability of a HIC purification strategy to provide “DAR = 1.0” and “DAR = 2.0” AJICAP™ ADCs with considerable potency and target selectivity.
Multicenter, Observational Cohort Study Evaluating Third-Generation Cephalosporin Therapy for Bloodstream Infections Secondary to Enterobacter, Serratia, and Citrobacter Species
Authors: Derrick Caroline , Bookstaver P. Brandon , Lu Zhiqiang K. , Bland Christopher M. , King S. Travis , Stover Kayla R. , Rumley Kathey , MacVane Shawn H. , Swindler Jenna , Kincaid Scott , Branan Trisha , Cluck David , Britt Benjamin , Pillinger Kelly E. , Jones Bruce M. , Fleming Virginia , DiMondi V. Paul , Estrada Sandy , Crane Brad , Odle Brian , Al-Hasan Majdi N. , Justo Julie Ann
Published: May 2020

Objectives: There is debate on whether the use of third-generation cephalosporins (3GC) increases the risk of clinical failure in bloodstream infections (BSIs) caused by chromosomally-mediated AmpC-producing Enterobacterales (CAE). This study evaluates the impact of definitive 3GC therapy versus other antibiotics on clinical outcomes in BSIs due to Enterobacter, Serratia, or Citrobacter species. Methods: This multicenter, retrospective cohort study evaluated adult hospitalized patients with BSIs secondary to Enterobacter, Serratia, or Citrobacter species from 1 January 2006 to 1 September 2014. Definitive 3GC therapy was compared to definitive therapy with other non-3GC antibiotics. Multivariable Cox proportional hazards regression evaluated the impact of definitive 3GC on overall treatment failure (OTF) as a composite of in-hospital mortality, 30-day hospital readmission, or 90-day reinfection. Results: A total of 381 patients from 18 institutions in the southeastern United States were enrolled. Common sources of BSIs were the urinary tract and central venous catheters (78 (20.5%) patients each). Definitive 3GC therapy was utilized in 65 (17.1%) patients. OTF occurred in 22/65 patients (33.9%) in the definitive 3GC group vs. 94/316 (29.8%) in the non-3GC group (p = 0.51). Individual components of OTF were comparable between groups. Risk of OTF was comparable with definitive 3GC therapy vs. definitive non-3GC therapy (aHR 0.93, 95% CI 0.51–1.72) in multivariable Cox proportional hazards regression analysis. Conclusions: These outcomes suggest definitive 3GC therapy does not significantly alter the risk of poor clinical outcomes in the treatment of BSIs secondary to Enterobacter, Serratia, or Citrobacter species compared to other antimicrobial agents.
Optimizing Anti-Viral Vaccine Responses: Input from a Non-Specialist
Authors: Serwer Philip
Published: May 2020

Recently, the research community has had a real-world look at reasons for improving vaccine responses to emerging RNA viruses. Here, a vaccine non-specialist suggests how this might be done. I propose two alternative options and compare the primary alternative option with current practice. The basis of comparison is feasibility in achieving what we need: a safe, mass-produced, emerging virus-targeted vaccine on 2–4 week notice. The primary option is the following. (1) Start with a platform based on live viruses that infect bacteria, but not humans (bacteriophages, or phages). (2) Isolate phages (to be called pathogen homologs) that resemble and provide antigenic context for membrane-covered, pathogenic RNA viruses; coronavirus-phage homologs will probably be found if the search is correctly done. (3) Upon isolating a viral pathogen, evolve its phage homolog to bind antibodies neutralizing for the viral pathogen. Vaccinate with the evolved phage homolog by generating a local, non-hazardous infection with the phage host and then curing the infection by propagating the phage in the artificially infecting bacterial host. I discuss how this alternative option has the potential to provide what is needed after appropriate platforms are built.
One-Step Differential Detection of OXA-48-Like Variants Using High-Resolution Melting (HRM) Analysis
Authors: Lau Min Yi , Abdul Jabar Kartini , Chua Kek Heng , Kee Boon Pin , Ponnampalavanar Sasheela Sri La Sri , Chong Chun Wie , Teh Cindy Shuan Ju
Published: May 2020

OXA-48-like carbapenemase gene remains a hidden threat, as different OXA-48 variants have varying presentations of susceptibility to antibiotics that might affect the treatment decisions. Rapid detection and differentiation of OXA-48-like carbapenemase genes are critical for targeted treatment and infection control. In this study, we aimed to develop high-resolution melting (HRM) analysis for the differentiation of OXA-48 variants. HRM analysis is a post-polymerase chain reaction (post-PCR) method for identification of small variations in nucleic acid sequences based on the PCR dissociation curve. A total of 82 bacterial strains, which consisted of Enterobacteriaceae and non-Enterobacteriaceae, were collected from a tertiary teaching hospital. The sensitivity and specificity of the assay were determined, and the developed assay was evaluated using the collected isolates against conventional-sequencing method. Overall, the developed assay was able to detect isolates that harboured OXA-48 and OXA232/OXA-181 by showing two distinct peaks at 81.1 ± 0.2 °C and 82.1 ± 0.2 °C, respectively. The detection limit of the assay was 1.6 x 10−6 ng/µL for OXA-48 and 1.8 × 10−7 ng/µL for OXA-232/OXA-181. This assay showed 100% specificity when evaluated on a panel of 37 isolates comprised of different species of bacteria and yeasts. When the assay with isolates collected in the year 2016 was first evaluated, the assay showed comparable results with conventional PCR-sequencing method where 34 OXA-48 and OXA-232/OXA-181 were detected. By using HRM analysis, the presence of OXA-48-like variants could be easily identified within 3 h from the pure culture.
Detection of Quorum-Sensing Molecules for Pathogenic Molecules Using Cell-Based and Cell-Free Biosensors
Authors: Miller Craig , Gilmore Jordon
Published: May 2020

Since the discovery and subsequent use of penicillin, antibiotics have been used to treat most bacterial infections in the U.S. Over time, the repeated prescription of many antibiotics has given rise to many antibiotic-resistant microbes. A bacterial strain becomes resistant by horizontal gene transfer, where surviving microbes acquire genetic material or DNA fragments from adjacent bacteria that encode for resistance. In order to avoid significant bacterial resistance, novel and target therapeutics are needed. Further advancement of diagnostic technologies could be used to develop novel treatment strategies. The use of biosensors to detect quorum-sensing signaling molecules has the potential to provide timely diagnostic information toward mitigating the multidrug-resistant bacteria epidemic. Resistance and pathogenesis are controlled by quorum-sensing (QS) circuits. QS systems secrete or passively release signaling molecules when the bacterial concentration reaches a certain threshold. Signaling molecules give an early indication of virulence. Detection of these compounds in vitro or in vivo can be used to identify the onset of infection. Whole-cell and cell-free biosensors have been developed to detect quorum-sensing signaling molecules. This review will give an overview of quorum networks in the most common pathogens found in chronic and acute infections. Additionally, the current state of research surrounding the detection of quorum-sensing molecules will be reviewed. Followed by a discussion of future works toward the advancement of technologies to quantify quorum signaling molecules in chronic and acute infections.
Antagonistic Activity against Ascosphaera apis and Functional Properties of Lactobacillus kunkeei Strains
Authors: Iorizzo Massimo , Lombardi Silvia Jane , Ganassi Sonia , Testa Bruno , Ianiro Mario , Letizia Francesco , Succi Mariantonietta , Tremonte Patrizio , Vergalito Franca , Cozzolino Autilia , Sorrentino Elena , Coppola Raffaele , Petrarca Sonia , Mancini Massimo , De Cristofaro Antonio
Published: May 2020

Lactic acid bacteria (LAB) are an important group of honeybee gut microbiota. These bacteria are involved in food digestion, stimulate the immune system, and may antagonize undesirable microorganisms in the gastrointestinal tract. Lactobacillus kunkeei is a fructophilic lactic acid bacterium (FLAB) most frequently found in the gastrointestinal tracts of honeybees. Ascosphaera apis is an important pathogenic fungus of honeybee larvae; it can colonize the intestine, especially in conditions of nutritional or environmental stress that cause microbial dysbiosis. In this work, some functional properties of nine selected L. kunkeei strains were evaluated. The study focused on the antifungal activity of these strains against A. apis DSM 3116, using different matrices: cell lysate, broth culture, cell-free supernatant, and cell pellet. The cell lysate showed the highest antifungal activity. Moreover, the strains were shown to possess good cell-surface properties (hydrophobicity, auto-aggregation, and biofilm production) and a good resistance to high sugar concentrations. These L. kunkeei strains were demonstrated to be functional for use in “probiotic syrup”, useful to restore the symbiotic communities of the intestine in case of dysbiosis and to exert a prophylactic action against A. apis.
Resveratrol Regulates the Expression of Genes Involved in CoQ Synthesis in Liver in Mice Fed with High Fat Diet
Authors: Meza-Torres Catherine , Hernández-Camacho Juan Diego , Cortés-Rodríguez Ana Belén , Fang Luis , Bui Thanh Tung , Rodríguez-Bies Elisabet , Navas Plácido , López-Lluch Guillermo
Published: May 2020

Resveratrol (RSV) is a bioactive natural molecule that induces antioxidant activity and increases protection against oxidative damage. RSV could be used to mitigate damages associated to metabolic diseases and aging. Particularly, RSV regulates different aspects of mitochondrial metabolism. However, no information is available about the effects of RSV on Coenzyme Q (CoQ), a central component in the mitochondrial electron transport chain. Here, we report for the first time that RSV modulates COQ genes and parameters associated to metabolic syndrome in mice. Mice fed with high fat diet (HFD) presented a higher weight gain, triglycerides (TGs) and cholesterol levels while RSV reverted TGs to control level but not weight or cholesterol. HFD induced a decrease of COQs gene mRNA level, whereas RSV reversed this decrease in most of the COQs genes. However, RSV did not show effect on CoQ9, CoQ10 and total CoQ levels, neither in CoQ-dependent antioxidant enzymes. HFD influenced mitochondrial dynamics and mitophagy markers. RSV modulated the levels of PINK1 and PARKIN and their ratio, indicating modulation of mitophagy. In summary, we report that RSV influences some of the metabolic adaptations of HFD affecting mitochondrial physiology while also regulates COQs gene expression levels in a process that can be associated with mitochondrial dynamics and turnover.
In Vitro Biological Activities of Fruits and Leaves of Elaeagnus multiflora Thunb. and Their Isoprenoids and Polyphenolics Profile
Authors: Lachowicz Sabina , Kapusta Ireneusz , Świeca Michał , Stinco Carla M. , Meléndez-Martínez Antonio J. , Bieniek Anna
Published: May 2020

The objective of this study was in-depth identification of carotenoids and polyphenolic compounds in leaves and fruits of Elaeagnus multiflora Thunb. An additional aim was to assay their antioxidant and in vitro biological activities (the ability to inhibit pancreatic lipase, α-amylase, and α-glucosidase activity) of two cultivars: ‘Sweet Scarlet’ and ‘Jahidka’. Study results showed the presence of 70 bioactive compounds, including 20 isoprenoids and 50 polyphenols. The profile of identified bioactive compounds had not been examined in this respect until now. The total carotenoid, chlorophyll, and polyphenol levels and antioxidant activity of the foliar samples were virtually identical in both cultivars and clearly higher relative to those in the fruits. On the other hand, the ability to inhibit pancreatic lipase, α-amylase, and α-glucosidase activity of the fruits was clearly higher as compared to the leaves. The highest amount of phenolic acids, flavonols, and polymeric procyanidins was in the ‘Sweet Scarlet’ for fruit and leaves, while the highest amount of chlorophylls and carotenoids was in the ‘Jahidka’. The inhibition of α-amylase, α-glucosidase, and pancreatic lipase activities appeared to be better correlated with the carotenoid content, which warrants further studies of the possible anti-diabetic and anti-obesity actions of the major carotenoids found in the fruits (lycopene, phytoene, and lutein). In addition, strong correlation between antioxidant activity and phenols of E. multiflora Thunb. components can be effective in removing reactive oxygen species. The results of our study show that both the fruits and leaves of E. multiflora Thunb. can be important for health promotion through the diet and for innovating in the industry of functional food and (nutri)cosmetics.
Water Extract from Inflorescences of Industrial Hemp Futura 75 Variety as a Source of Anti-Inflammatory, Anti-Proliferative and Antimycotic Agents: Results from In Silico, In Vitro and Ex Vivo Studies
Authors: Orlando Giustino , Recinella Lucia , Chiavaroli Annalisa , Brunetti Luigi , Leone Sheila , Carradori Simone , Di Simone Simonetta , Ciferri Maria Chiara , Zengin Gokhan , Ak Gunes , Abdullah Hassan H. , Cordisco Estefanía , Sortino Maximiliano , Svetaz Laura , Politi Matteo , Angelini Paola , Covino Stefano , Venanzoni Roberto , Cesa Stefania , Menghini Luigi , Ferrante Claudio
Published: May 2020

Industrial hemp (Cannabis sativa) is traditionally cultivated as a valuable source of fibers and nutrients. Multiple studies also demonstrated antimicrobial, anti-proliferative, phytotoxic and insecticide effects of the essential oil from hemp female inflorescences. On the other side, only a few studies explored the potential pharmacological application of polar extracts from inflorescences. In the present study, we investigated the water extract from inflorescences of industrial hemp Futura 75 variety, from phytochemical and pharmacological point of view. The water extract was assayed for phenolic compound content, radical scavenger/reducing, chelating and anti-tyrosinase effects. Through an ex vivo model of toxicity induced by lipopolysaccharide (LPS) on isolated rat colon and liver, we explored the extract effects on serotonin, dopamine and kynurenine pathways and the production of prostaglandin (PG)E2. Anti-proliferative effects were also evaluated against human colon cancer HCT116 cell line. Additionally, antimycotic effects were investigated against Trichophyton rubrum, Trichophyton interdigitale, Microsporum gypseum. Finally, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted in order to predict the putative targets underlying the observed pharmacological and microbiological effects. Futura 75 water extract was able to blunt LPS-induced reduction of serotonin and increase of dopamine and kynurenine turnover, in rat colon. Additionally, the reduction of PGE2 levels was observed in both colon and liver specimens, as well. The extract inhibited the HCT116 cell viability, the growth of T. rubrum and T. interdigitale and the activity of tyrosinase, in vitro, whereas in silico studies highlighting the inhibitions of cyclooxygenase-1 (induced by carvacrol), carbonic anhydrase IX (induced by chlorogenic acid and gallic acid) and lanosterol 14-α-demethylase (induced by rutin) further support the observed pharmacological and antimycotic effects. The present findings suggest female inflorescences from industrial hemp as high quality by-products, thus representing promising sources of nutraceuticals and cosmeceuticals against inflammatory and infectious diseases.
A Basic Study on Sound Control System for Ultra-Compact Electric Vehicle by Using Masking
Authors: Kato Taro , Nakayama Hiroya , Kato Hideaki , Narita Takayoshi
Published: May 2020

In this study, we conducted a quantitative evaluation of the comfort of the interior of an electric vehicle (EV) using the brain wave appearance rate, which is part of the human biologic information that the initial stage of the proposed active noise control (ANC) system for ultra-compact EVs reveals. EVs have become easy-to-use mobility solutions and have been researched and actively developed focusing on using music characteristics. We performed fundamental testing of music including 1/f fluctuation for the evaluation of ride comfort based on the relationship between the participant’s heart rate and tempo of music using a driving simulator. The results suggest that if a passenger listened to music including a 1/f fluctuation, then he/she could relax. Thus, it was concluded that if we could pre-grasp the passenger’s biologic information of the heart rate and beats per minute for masking, then the comfort in the interior space could be improved even when using a driving simulator.
Evaluation of Microbial Load, Formation of Odorous Metabolites and Lipid Stability during Wet Preservation of Nannochloropsis gaditana Concentrates
Authors: Verspreet Joran , Kreps Sabine , Bastiaens Leen
Published: May 2020

Wet preservation of algae allows us to bridge the time period between algae harvest and processing while avoiding the costs and nutritional losses associated with algae drying. This study aimed to identify suitable storage conditions for the wet preservation of Nannochloropsis gaditana concentrates. The impact of storage temperature, time and the way of closing the storage recipient was evaluated using a full factorial design. The effect of acetic acid addition was tested for one storage condition. Storage temperature was the main factor determining the microbial count and had a vast impact on the formation of odorous metabolites. Storage at 20 °C in closed recipients led to rapid O2 consumption, accumulation of malodorous short-chain fatty acids above their odor thresholds, and the production of H2S and methanethiol. These odorous metabolites were not formed or to a much lower extent during 4 °C and 8 °C storage in closed recipients. Acetic acid supplementation (50 mM) suppressed the formation of short-chain fatty acids during 8 °C storage in unsealed recipients and reduced the aerobic microbial count and the number of yeasts and molds by approximately one log unit after 14 days. Yet, acetic acid addition also induced lipid hydrolysis and decreased chlorophyll levels when algae were stored for more than one week. This study demonstrated that temperature control is needed and that acetic acid addition is a promising approach when N. gaditana concentrates are stored for less than one week.
Bioactive Glasses and Glass/Polymer Composites for Neuroregeneration: Should We Be Hopeful?
Authors: Kargozar Saeid , Mozafari Masoud , Ghenaatgar-Kasbi Maryam , Baino Francesco
Published: May 2020

Bioactive glasses (BGs) have been identified as highly versatile materials in tissue engineering applications; apart from being used for bone repair for many years, they have recently shown promise for the regeneration of peripheral nerves as well. They can be formulated in different shapes and forms (micro-/nanoparticles, micro-/nanofibers, and tubes), thus potentially meeting the diverse requirements for neuroregeneration. Mechanical and biological improvements in three-dimensional (3D) polymeric scaffolds could be easily provided by adding BGs to their composition. Various types of silicate, borate, and phosphate BGs have been examined for use in neuroregeneration. In general, BGs show good compatibility with the nervous system compartments both in vitro and in vivo. Functionalization and surface modification plus doping with therapeutic ions make BGs even more effective in peripheral nerve regeneration. Moreover, the combination of BGs with conductive polymers is suggested to improve neural cell functions at injured sites. Taking advantage of BGs combined with novel technologies in tissue engineering, like 3D printing, can open new horizons in reconstructive approaches for the nervous system. Although there are great potential opportunities in BG-based therapies for peripheral nerve regeneration, more research should still be performed to carefully assess the pros and cons of BGs in neuroregeneration strategies.
Automated Detection and Classification of Defective and Abnormal Dies in Wafer Images
Authors: Chen Hsiang-Chieh
Published: May 2020

This article presents an automated vision-based algorithm for the die-scale inspection of wafer images captured using scanning acoustic tomography (SAT). This algorithm can find defective and abnormal die-scale patterns, and produce a wafer map to visualize the distribution of defects and anomalies on the wafer. The main procedures include standard template extraction, die detection through template matching, pattern candidate prediction through clustering, and pattern classification through deep learning. To conduct the template matching, we first introduce a two-step method to obtain a standard template from the original SAT image. Subsequently, a majority of the die patterns are detected through template matching. Thereafter, the columns and rows arranged from the detected dies are predicted using a clustering method; thus, an initial wafer map is produced. This map is composed of detected die patterns and predicted pattern candidates. In the final phase of the proposed algorithm, we implement a deep learning-based model to determine defective and abnormal patterns in the wafer map. The experimental results verified the effectiveness and efficiency of our proposed algorithm. In conclusion, the proposed method performs well in identifying defective and abnormal die patterns, and produces a wafer map that presents important information for solving wafer fabrication issues.
A Customized VGG19 Network with Concatenation of Deep and Handcrafted Features for Brain Tumor Detection
Authors: Rajinikanth Venkatesan , Joseph Raj Alex Noel , Thanaraj Krishnan Palani , Naik Ganesh R.
Published: May 2020

Brain tumor (BT) is one of the brain abnormalities which arises due to various reasons. The unrecognized and untreated BT will increase the morbidity and mortality rates. The clinical level assessment of BT is normally performed using the bio-imaging technique, and MRI-assisted brain screening is one of the universal techniques. The proposed work aims to develop a deep learning architecture (DLA) to support the automated detection of BT using two-dimensional MRI slices. This work proposes the following DLAs to detect the BT: (i) implementing the pre-trained DLAs, such as AlexNet, VGG16, VGG19, ResNet50 and ResNet101 with the deep-features-based SoftMax classifier; (ii) pre-trained DLAs with deep-features-based classification using decision tree (DT), k nearest neighbor (KNN), SVM-linear and SVM-RBF; and (iii) a customized VGG19 network with serially-fused deep-features and handcrafted-features to improve the BT detection accuracy. The experimental investigation was separately executed using Flair, T2 and T1C modality MRI slices, and a ten-fold cross validation was implemented to substantiate the performance of proposed DLA. The results of this work confirm that the VGG19 with SVM-RBF helped to attain better classification accuracy with Flair (>99%), T2 (>98%), T1C (>97%) and clinical images (>98%).
Efficient Distributed Preprocessing Model for Machine Learning-Based Anomaly Detection over Large-Scale Cybersecurity Datasets
Authors: Larriva-Novo Xavier , Vega-Barbas Mario , Villagrá Víctor A. , Rivera Diego , Álvarez-Campana Manuel , Berrocal Julio
Published: May 2020

New computational and technological paradigms that currently guide developments in the information society, i.e., Internet of things, pervasive technology, or Ubicomp, favor the appearance of new intrusion vectors that can directly affect people’s daily lives. This, together with advances in techniques and methods used for developing new cyber-attacks, exponentially increases the number of cyber threats which affect the information society. Because of this, the development and improvement of technology that assists cybersecurity experts to prevent and detect attacks arose as a fundamental pillar in the field of cybersecurity. Specifically, intrusion detection systems are now a fundamental tool in the provision of services through the internet. However, these systems have certain limitations, i.e., false positives, real-time analytics, etc., which require their operation to be supervised. Therefore, it is necessary to offer architectures and systems that favor an efficient analysis of the data handled by these tools. In this sense, this paper presents a new model of data preprocessing based on a novel distributed computing architecture focused on large-scale datasets such as UGR’16. In addition, the paper analyzes the use of machine learning techniques in order to improve the response and efficiency of the proposed preprocessing model. Thus, the solution developed achieves good results in terms of computer performance. Finally, the proposal shows the adequateness of decision tree algorithms for training a machine learning model by using a large dataset when compared with a multilayer perceptron neural network.