bluuebunny/crossref_metadata_2025 huggingface dataset:
An Update on Antiviral Therapy Against SARS-CoV-2: How Far Have We Come?
Authors: Indari Omkar , Jakhmola Shweta , Manivannan Elangovan , Jha Hem Chandra
Published: March 2021

COVID-19 pandemic has spread worldwide at an exponential rate affecting millions of people instantaneously. Currently, various drugs are under investigation to treat an enormously increasing number of COVID-19 patients. This dreadful situation clearly demands an efficient strategy to quickly identify drugs for the successful treatment of COVID-19. Hence, drug repurposing is an effective approach for the rapid discovery of frontline arsenals to fight against COVID-19. Successful application of this approach has resulted in the repurposing of some clinically approved drugs as potential anti-SARS-CoV-2 candidates. Several of these drugs are either antimalarials, antivirals, antibiotics or corticosteroids and they have been repurposed based on their potential to negate virus or reduce lung inflammation. Large numbers of clinical trials have been registered to evaluate the effectiveness and clinical safety of these drugs. Till date, a few clinical studies are complete and the results are primary. WHO also conducted an international, multi-country, open-label, randomized trials-a solidarity trial for four antiviral drugs. However, solidarity trials have few limitations like no placebos were used, additionally any drug may show effectiveness for a particular population in a region which may get neglected in solidarity trial analysis. The ongoing randomized clinical trials can provide reliable long-term follow-up results that will establish both clinical safety and clinical efficacy of these drugs with respect to different regions, populations and may aid up to worldwide COVID-19 treatment research. This review presents a comprehensive update on majorly repurposed drugs namely chloroquine, hydroxychloroquine, remdesivir, lopinavir-ritonavir, favipiravir, ribavirin, azithromycin, umifenovir, oseltamivir as well as convalescent plasma therapy used against SARS-CoV-2. The review also summarizes the data recorded on the mechanism of anti-SARS-CoV-2 activity of these repurposed drugs along with the preclinical and clinical findings, therapeutic regimens, pharmacokinetics, and drug-drug interactions.
Skeletal Muscle and Bone – Emerging Targets of Fibroblast Growth Factor-21
Authors: Sun Hui , Sherrier Matthew , Li Hongshuai
Published: March 2021

Fibroblast growth factor 21 (FGF21) is an atypical member of the FGF family, which functions as a powerful endocrine and paracrine regulator of glucose and lipid metabolism. In addition to liver and adipose tissue, recent studies have shown that FGF21 can also be produced in skeletal muscle. As the most abundant tissue in the human body, skeletal muscle has become increasingly recognized as a major site of metabolic activity and an important modulator of systemic metabolic homeostasis. The function and mechanism of action of muscle-derived FGF21 have recently gained attention due to the findings of considerably increased expression and secretion of FGF21 from skeletal muscle under certain pathological conditions. Recent reports regarding the ectopic expression of FGF21 from skeletal muscle and its potential effects on the musculoskeletal system unfolds a new chapter in the story of FGF21. In this review, we summarize the current knowledge base of muscle-derived FGF21 and the possible functions of FGF21 on homeostasis of the musculoskeletal system with a focus on skeletal muscle and bone.
Duckweed Species Genotyping and Interspecific Hybrid Discovery by Tubulin-Based Polymorphism Fingerprinting
Authors: Braglia Luca , Lauria Massimiliano , Appenroth Klaus J. , Bog Manuela , Breviario Diego , Grasso Aldo , Gavazzi Floriana , Morello Laura
Published: March 2021

Duckweeds (Lemnaceae) are the smallest and fastest-growing angiosperms. This feature, together with high starch production and good nutritional properties, makes them suitable for several applications, including wastewater treatment, bioenergy production, or feed and food supplement. Due to their reduced morphology and great similarity between diverse species, taxonomic identification of duckweeds is a challenging issue even for experts. Among molecular genotyping methods, DNA barcoding is the most useful tool for species identification without a need for cluster analysis. The combination of two plastid barcoding loci is now considered the gold standard for duckweed classification. However, not all species can be defined with confidence by these markers, and a fast identification method able to solve doubtful cases is missing. Here we show the potential of tubulin-based polymorphism (TBP), a molecular marker based on the intron length polymorphisms of β-tubulin loci, in the genomic profiling of the generaSpirodela,Landoltia, andLemna. Ninety-four clones were analyzed, including at least two representatives of each species of the three genera, with a special focus on the very heterogeneous speciesLemna minor. We showed that a single PCR amplification with universal primers, followed by agarose gel analysis, was able to provide distinctive fingerprinting profiles for 10 out of 15 species. Cluster analysis of capillary electrophoresis–TBP data provided good separation for the remaining species, although the relationship betweenL. minorandLemna japonicawas not fully resolved. However, an accurate comparison of TBP profiles provided evidence for the unexpected existence of intraspecific hybrids betweenLemna turioniferaandL. minor, as further confirmed by amplified fragment length polymorphism and sequence analysis of a specific β-tubulin locus. Such hybrids could possibly correspond toL. japonica, as originally suggested by E. Landolt. The discovery of interspecific hybrids opens a new perspective to understand the speciation mechanisms in the family of duckweeds.
Heat Shock Protein HSP24 Is Involved in the BABA-Induced Resistance to Fungal Pathogen in Postharvest Grapes Underlying an NPR1-Dependent Manner
Authors: Li Chunhong , Cao Shifeng , Wang Kaituo , Lei Changyi , Ji Nana , Xu Feng , Jiang Yongbo , Qiu Linglan , Zheng Yonghua
Published: March 2021

Although heat shock proteins (HSPs), a family of ubiquitous molecular chaperones, are well characterized in heat stress-related responses, their function in plant defense remains largely unclear. Here, we report the role of VvHSP24, a class B HSP from Vitis vinifera, in β-aminobutyric acid (BABA)-induced priming defense against the necrotrophic fungus Botrytis cinerea in grapes. Grapes treated with 10 mmol L–1 BABA exhibited transiently increased transcript levels of VvNPR1 and several SA-inducible genes, including PR1, PR2, and PR5. Additionally, phytoalexins accumulated upon inoculation with the gray mold fungus B. cinerea, which coincided with the action of a priming mode implicated in pathogen-driven resistance. Intriguingly, electrophoretic mobility shift (EMSA), yeast two-hybrid (Y2H) and His pull-down assays demonstrated that the nuclear chaperone VvHSP24 cannot modulate the transcript of PR genes but does directly interact with VvNPR1 in vivo or in vitro. Furthermore, we found that VvHSP24 overexpression enhanced the transcript levels of NPR1 and SA-responsive genes (PR1, PR2, and PR5) and increased the resistance of transgenic Arabidopsis thaliana to B. cinerea compared with wildtype Col-0. An opposite trend between CRISPR mutants of AtHSFB1 (the orthologous gene of VvHSP24 in Arabidopsis) and wildtype plants was observed. Hence, our results suggest that VvHSP24 has a potential role in NPR1-dependent plant resistance to fungal pathogen. BABA-induced priming defense in grapes may require posttranslational modification of the chaperone VvHSP24 to activate VvNPR1 transcript, leading to PR gene expressions and resistance phenotypes.
Assessing 16 Fundamental Motives With Fewer Than 50 Items: Development and Validation of the German 16 Motives Research Scales (16mrs)
Authors: Dörendahl Jan , Greiff Samuel , Niepel Christoph
Published: March 2021

Psychometrically sound short scales are required to comprehensively and yet economically assess fundamental motives in research settings such as large-scale assessments. In order to provide such a time- and cost-efficient instrument, we conducted three studies (N = 1,568) to develop further and validate 16 German scales with three items each assessing fundamental motives [16 motives research scales (16mrs)]. In Study 1, we applied a top–down construction process to develop a preliminary item pool on the basis of a thorough revision of existing construct definitions. In Study 2, we chose an approach that allowed us to balance the optimization of psychometric properties with content coverage to select three-item scales for each of the 16 motives. For the item selection process, we combined exploratory factor analyses, confirmatory factor analyses, ant colony optimization algorithm, and Mokken scale analyses. In Study 3, we cross-validated the results obtained in Study 2 and placed the 16mrs in a nomological network consisting of Big Five traits and Power, Achievement, Affiliation, Intimacy, and Fear motives. The results of these studies indicate that the 16mrs can be used to reliably and validly assess fundamental motives that represent a level of personality that differs from the Big Five and covers motivational aspects beyond the well-established Power, Achievement, Affiliation, Intimacy, and Fear motives. Limitations concerning the reliability of the Autonomy scale and the empirical discrimination of the Dominance and Status scales are discussed. In addition to the validated German version, we also provide the English translation of the items, which, however, need to be validated before use.
Stressful Life Events and Resilience During the COVID-19 Lockdown Measures in Italy: Association With Mental Health Outcomes and Age
Authors: Rossi Rodolfo , Jannini Tommaso B. , Socci Valentina , Pacitti Francesca , Lorenzo Giorgio Di
Published: March 2021

Background: The COVID-19 pandemic, due to its disproportionated higher morbidity and mortality rates in the older age, has been considered to be a “geropandemic.” Several studies, however, have found that older age is associated with lower psychological distress in relation to the COVID-19 outbreak and related lockdown measures.

Aim: To explore the role of Resilience as a mediator between stressful COVID-19 related life events and depressive and, anxiety symptoms and perceived stress, and to ascertain the role of age as a moderator of the mediator's effect.

Methods: An on-line survey was spread through social networks during the first lockdown in Italy. Depressive and anxiety symptoms and perceived stress were measured using the Italian version of the Patient Health Questionnaire-9 (PHQ-9), the Generalized Anxiety Disorder Questionnaire-7 (GAD-7) and the Perceived Stress Scale (PSS). Resilience was measured using the Italian version of the Resilience Scale for Adults (RSA). Stressful COVID-19 related life events were explored using a checklist of events derived from the International Adjustment Disorder Questionnaire (IADQ). After a preliminary panel of linear regressions, mediation was tested using Structural Equation Modeling and inspecting the bootstrapped indirect effects. Afterwards, age was introduced as a mediator of the indirect effect in a moderated mediation analysis.

Results: Twenty one thousand three hundred and thirty four subjects completed the questionnaire, 17,178 (80.52%) were female, 748 (3.5%) were >60 years old. In the whole sample, the presence of any stressful event was associated with depressive and anxiety symptoms and perceived stress. Resilience mediated the effects of stressful COVID-19-related events on depressive and anxiety symptoms and perceived stress. The moderated mediation analysis revealed that age moderated the mediation effect of Resilience between the presence of a stressful event and the selected outcomes.

Conclusion: Taken together, our results show that age moderates the mediating effect of Resilience in the relationship between COVID-19-related stressful events and depressive and anxiety symptoms and perceived stress. Older adults' Resilience was less influenced by stressful events, and this could be one of the reasons accounting for the better mental health outcomes observed in the older age.
Prevalence and Factors Associated With Body Pain: Results of a Nationally Representative Survey of 9,586 Chinese Adults Aged 60 and Over
Authors: Yang Lu , Peng Wenbo
Published: March 2021

Background: Pain management has become a critical problem worldwide with the aging population. More than half of older people have experienced pain with different severity. The aim of this research is to identify the characteristics of older people with body pain and the associations between pain and characteristics of demographic, health status, and health services use amongst Chinese seniors.

Methods: This cross-sectional study was based on the China Health and Retirement Longitudinal Study (CHARLS), using follow-up survey data in 2015. The national survey comprised 20,284 women and men aged 45 years or older who completed questionnaires. Data of older people who were asked whether they had troubles with body pain were extracted and analyzed. Multiple logistic regression modeling was used to determine the important indicators (demographic, health status, and health services use) amongst Chinese elderly with pain.

Results: Analyses revealed that 32.5% (n = 9,586) of Chinese people aged over 60 reported having body pain. Pain is positively associated with female gender (OR = 2.08, 95% CI 1.80–2.39, p < 0.001), living in non-urban areas (OR = 1.49, 95% CI 1.25–1.77, p < 0.001), having physical disabilities (OR = 1.45, 95% CI 1.15–1.82, p = 0.002), diagnosed with stomach diseases (OR = 1.40, 95% CI 1.20–1.64, p < 0.001), diagnosed with arthritis (OR = 1.91, 95% CI 1.66–2.20, p < 0.001), self-rating with poor health status (OR = 7.03, 95% CI 5.63–8.78, p < 0.001), self-purchased over-the-counter western medications (OR = 1.50, 95% CI 1.30–1.73, p < 0.001) and self-purchased Chinese herbal medicine (OR = 1.52, 95% CI 1.24–1.85, p < 0.001).

Conclusion: Body pain is common amongst the Chinese elderly. This research highlights the need for further nationwide studies exclusively focusing on people with pain including the elder population, and provides evidence-based insights for healthcare providers and policy-makers, to improve the quality of pain management. Future research should also pay attention to the importance of health literacy for health outcomes with regard to pain management.
The Study of Key Reactions Shaping the Post-Main Sequence Evolution of Massive Stars in Underground Facilities
Authors: Ferraro F. , Ciani G. F. , Boeltzig A. , Cavanna F. , Zavatarelli S.
Published: March 2021

The chemical evolution of the Universe and several phases of stellar life are regulated by minute nuclear reactions. The key point for each of these reactions is the value of cross-sections at the energies at which they take place in stellar environments. Direct cross-section measurements are mainly hampered by the very low counting rate and by cosmic background; nevertheless, they have become possible by combining the best experimental techniques with the cosmic silence of an underground laboratory. In the nineties, the LUNA (Laboratory for Underground Nuclear Astrophysics) collaboration opened the era of underground nuclear astrophysics, installing first a homemade 50 kV and, later on, a second 400 kV accelerator under the Gran Sasso mountain in Italy: in 25 years of experimental activity, important reactions responsible for hydrogen burning could have been studied down to the relevant energies thanks to the high current proton and helium beams provided by the machines. The interest in the next and warmer stages of star evolution (i.e., post-main sequence and helium and carbon burning) drove a new project based on an ion accelerator in the MV range called LUNA-MV, able to deliver proton, helium, and carbon beams. The present contribution is aimed to discuss the state of the art for some selected key processes of post-main sequence stellar phases:12C(α,γ)16O and12C+12C are fundamental for helium and carbon burning phases, and13C(α,n)16O and22Ne(α,n)25Mg are relevant to the synthesis of heavy elements in AGB stars. The perspectives opened by an underground MV facility will be highlighted.
Characterization of Anti-Müllerian Hormone (AMH) Gene in Buffaloes and Goats
Authors: Gautam Devika , Vats Ashutosh , Pal Prasanna , Haldar Avijit , De Sachinandan
Published: March 2021

The Anti-Müllerian Hormone (AMH) is a member of the transforming growth factor beta (TGF-β) superfamily, playing a significant role in cell proliferation, differentiation and apoptosis. In females, AMH is secreted throughout their reproductive life span from ovaries, whereas in males it is secreted by gonadal cells at a very early stage of testicular development. AMH is a promising marker of ovarian reserve in women and can be used to measure the female reproductive lifespan. In the present study, we cloned and sequenced the GC richAMHgene from Indian riverine buffalo (Bubalus bubalis)and goat (Capra hircus). Obtained sequences were compared to the AMH sequences of other mammals, and corresponding amino acid sequences revealed that the caprine and bovine AMH sequences are more closely related to each other than to those of other mammals. Furthermore, we analyzed the chromosomal localization ofAMHgenes in mammalian species to understand potential syntenic relationship. TheAMHgene is localized between the sequences for theSF3AandJSRP1genes and maintains this precise location in relation to other nearby genes. The dN/dS ratio ofAMHgene did not indicate any pressure for either positive or negative selection; thus, the physiological function of theAMHgene in the reproduction of these two ruminant species remains very vital. Similar to other mammals, theAMHgene may be an important indicator for regulating female reproductive biology function in bovine, cetacean, caprine, and camelidae.
Hypophosphatemia in Dogs With Presumptive Sepsis: A Retrospective Study (2008–2018)
Authors: Chu Victoria , Goggs Robert , Bichoupan Allison , Radhakrishnan Shalini , Menard Julie
Published: March 2021

Background: In humans with sepsis, hypophosphatemia is a marker of illness severity and a negative prognostic indicator. Hypophosphatemia has not been previously investigated in dogs with sepsis, however. This study aimed to estimate the prevalence of hypophosphatemia in dogs, the prevalence of presumptive sepsis in dogs with hypophosphatemia, the prevalence of hypophosphatemia in dogs with presumptive sepsis and the association between outcome and hypophosphatemia in dogs with presumptive sepsis.

Methods: Electronic medical records of the Cornell University Hospital for Animals from 2008–2018 were queried to identify all dogs with hypophosphatemia and all dogs with presumptive sepsis. Hypophosphatemia was defined as a serum phosphate concentration <2.7 mg/dL. Sepsis was presumed where ≥2 of 4 systemic inflammatory response syndrome (SIRS) criteria were satisfied associated with a documented or highly suspected infection. Variables were assessed for normality using the D'Agostino-Pearson test. Continuous variables were compared between groups using the Mann-Whitney U test. Differences in frequency between categorical variables were analyzed using contingency tables, calculation of Fisher's exact test or Chi2 and estimation of odds ratios.

Results: In the study period, 47,992 phosphate concentration measurements from 23,752 unique dogs were identified. After eliminating repeat analyses, the period prevalence of hypophosphatemia on a per dog basis over the 11-year study period was 10.6% (2,515/23,752). The prevalence of presumptive sepsis within dogs with hypophosphatemia was 10.7% (268/2,515). During the 11-year study period, 4,406 dogs with an infection were identified, of which 1,233 were diagnosed with presumptive sepsis and had a contemporaneous phosphate concentration. Hypophosphatemia was more prevalent in dogs with presumptive sepsis than in dogs without 21.7 vs. 10.2%; OR 2.44 [95% CI 2.12–2.81]; P < 0.0001. The mortality rate was greater in dogs with hypophosphatemia and presumptive sepsis than in dogs with hypophosphatemia without presumptive sepsis (15.3 vs. 3.1%; OR 5.70 [95% CI 3.76–8.52]; P < 0.0001), however hypophosphatemia was not associated with outcome in dogs with presumptive sepsis OR 0.87 [95% CI 0.60–1.26]; P = 0.518.

Conclusions: In dogs with hypophosphatemia, a presumed diagnosis of sepsis was associated with increased mortality compared to other associated disease processes. In dogs with presumptive sepsis, hypophosphatemia was not associated with outcome.
Spoken Instruction Understanding in Air Traffic Control: Challenge, Technique, and Application
Authors: Lin Yi
Published: March 2021

In air traffic control (ATC), speech communication with radio transmission is the primary way to exchange information between the controller and aircrew. A wealth of contextual situational dynamics is embedded implicitly; thus, understanding the spoken instruction is particularly significant to the ATC research. In this paper, a comprehensive review related to spoken instruction understanding (SIU) in the ATC domain is provided from the perspective of the challenges, techniques, and applications. Firstly, a full pipeline is represented to achieve the SIU task, including automatic speech recognition, language understanding, and voiceprint recognition. A total of 10 technique challenges are analyzed based on the ATC task specificities. In succession, the common techniques for SIU tasks are categorized from common applications, and extensive works in the ATC domain are also reviewed. Finally, a series of future research topics are also prospected based on the corresponding challenges. The author sincerely hopes that this work is able to provide a clear technical roadmap for the SIU tasks in the ATC domain and further make contributions to the research community.
Numerical Investigation of an Optimized Rotor Head Fairing for the RACER Compound Helicopter in Cruise Flight
Authors: Pölzlbauer Patrick , Kümmel Andreas , Desvigne Damien , Breitsamter Christian
Published: March 2021

The present work is part of the Clean Sky 2 project Full-Fairing Rotor Head Aerodynamic Design Optimization (FURADO), which deals with the aerodynamic design optimization of a full-fairing rotor head for the Rapid And Cost-Effective Rotorcraft (RACER) compound helicopter. The rotor head is a major drag source and previous investigations have revealed that the application of rotor head fairings can be an effective drag reduction measure. As part of the full-fairing concept, a new blade-sleeve fairing was aerodynamically optimized for cruise flight. Within this publication, the newly developed blade-sleeve fairing is put to test on an isolated, five-bladed rotor head and compared to an already existing reference blade-sleeve fairing, which was developed at Airbus Helicopters. Numerical flow simulations are performed with ANSYS Fluent 2019 R2 considering a rotating rotor head with cyclic pitch movement. The aerodynamic forces of the isolated rotor head are analyzed to determine the performance benefit of the newly developed blade-sleeve fairing. A drag reduction of 4.7% and a lift increase of 20% are obtained in comparison to the Airbus Helicopters reference configuration. Furthermore, selected surface and flow field quantities are presented to give an overview on the occurring flow phenomena.
S-Like Ribonuclease T2 Genes Are Induced during Mobilisation of Nutrients in Cotyledons from Common Bean
Authors: Diaz-Baena Mercedes , Delgado-García Elena , Pineda Manuel , Galvez-Valdivieso Gregorio , Piedras Pedro
Published: March 2021

Germination and seedling development are crucial phases in a plant’s life cycle with economical and agronomical implications. The RNA quality in seeds is linked to seed viability, being an important agronomic trait since this leads to a loss in germination efficiency. In addition, RNA can be an important phosphorous reservoir in seeds, affecting the efficiency of the mobilisation of nutrients towards the seedlings. However, knowledge about the physiological function of ribonucleases during germination and seedling development is scarce. We analysed the ribonuclease activities of cotyledons during these processes and the expression of S-like ribonucleases T2. Ribonuclease activity was detected in cotyledons at 1 day after imbibition and the specific activity increased during germination and seedling development, reaching a maximal value at 10 days after imbibition. At this stage, the levels of proteins and RNA in cotyledons were very low. Using in-gel assays, three ribonucleases were detected with apparent molecular masses of 16, 17 and 19 kDa along cotyledon ontogeny. The S-like ribonucleases T2 family consists of four genes in common bean (PvRNS1 to PvRNS4). The expression of PvRNS1, PvRNS2 and PvRNS4 increased in the phase of nutrient mobilisation in cotyledons. The expression of PvRNS1 increased 1000 fold in cotyledons, from 1 to 6 days after imbibition. The suppression of the induction of ribonuclease activity and gene expression in decapitated seedlings suggests that the regulatory signal comes from the developing axes. These results clearly state that S-like ribonucleases T2 are involved in RNA turnover in cotyledons during seedling development.
Confirmation of Multiple Resistant Chloris radiata Population, Harvested in Colombian Rice Fields
Authors: Hoyos Verónica , Plaza Guido , Vázquez-Garcia José G. , Palma-Bautista Candelario , Rojano-Delgado Antonia M. , De Prado Rafael
Published: March 2021

This paper reports the first C. radiata population with resistance to glyphosate and multiple resistance to the acetolactate synthase (ALS) inhibitor, imazamox. Two populations, one putative resistant (R) and one susceptible (S), were used in the studies. Dose–response experiments were performed to evaluate the resistance factor (RF). Shikimic acid accumulation, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and ALS enzyme activities were studied together with chemical integrated weed management (adjuvants and alternative herbicides). The resistance to glyphosate and imazamox was confirmed based on the dry weight reduction, visual evaluation and survival. The results of dose–response curve assays showed for the R population intermedium RF for glyphosate (5.1 and 9.7 for amount of herbicide needed to reduce the dry weight by 50% GR50 and lethal dose of 50% LD50, respectively) and high RF for imazamox (34.9 and 37.4, respectively). The low shikimic acid accumulation in R population confirmed the glyphosate resistance. The glyphosate concentration which inhibited the EPSPS enzyme in 50% (I50) was approximately 20 times higher for R population than the S population, while the imazamox I50 in ALS enzyme for the R plants was 89 times greater than the S plants. In the chemical integrated weed management, the foliar retention and effectivity assays showed that the use of adjuvants improves the retention of glyphosate and imazamox, and the reduction in dry weight of weeds. The alternative herbicides study showed that the acetyl-CoA carboxylase (ACCase) inhibitors, paraquat and glufosinate, had better results for control in this species. However, poor control was observed with bispyribac-sodium, metsulfuron-methyl and quinclorac, indicating possible cross-resistance for ALS-inhibitors and also multiple resistance for auxinic herbicides (quinclorac). Nevertheless dose–response experiments are required to confirm this assumption.
Allium Extract Implements Weaned Piglet’s Productive Parameters by Modulating Distal Gut Microbiota
Authors: Rabelo-Ruiz Miguel , Teso-Pérez Claudia , Peralta-Sánchez Juan Manuel , Ariza Juan José , Martín-Platero Antonio Manuel , Casabuena-Rincón Óscar , Vázquez-Chas Patricia , Guillamón Enrique , Aguinaga-Casañas María Arántzazu , Maqueda Mercedes , Valdivia Eva , Baños Alberto , Martínez-Bueno Manuel
Published: March 2021

Antimicrobial resistance (AMR) has risen as a global threat for human health. One of the leading factors for this emergence has been the massive use of antibiotics growth-promoter (AGPs) in livestock, enhancing the spread of AMR among human pathogenic bacteria. Thus, several alternatives such as probiotics, prebiotics, or phytobiotics have been proposed for using in animal feeding to maintain or improve productive levels while diminishing the negative effects of AGPs. Reducing the use of antibiotics is a key aspect in the pig rearing for production reasons, as well as for the production of high-quality pork, acceptable to consumers. Here we analyze the potential use of Allium extract as an alternative. In this study, weaned piglets were fed with Allium extract supplementation and compared with control and antibiotic (colistin and zinc oxide) treated piglets. The effects of Allium extract were tested by analyzing the gut microbiome and measuring different productive parameters. Alpha diversity indices decreased significantly in Allium extract group in caecum and colon. Regarding beta diversity, significant differences between treatments appeared only in caecum and colon. Allium extract and antibiotic piglets showed better values of body weight (BW), average daily weight gain (ADG), and feed conversion ratio (FCR) than control group. These results indicate that productive parameters can be implemented by modifying the gut microbiota through phytobiotics such as Allium extract, which will drive to drop the use of antibiotics in piglet diet.
ACE Inhibition Modulates Myeloid Hematopoiesis after Acute Myocardial Infarction and Reduces Cardiac and Vascular Inflammation in Ischemic Heart Failure
Authors: Rudi Wolf-Stephan , Molitor Michael , Garlapati Venkata , Finger Stefanie , Wild Johannes , Münzel Thomas , Karbach Susanne H. , Wenzel Philip
Published: March 2021

Aims: Angiotensin-converting-enzyme inhibitors (ACE inhibitors) are a cornerstone of drug therapy after myocardial infarction (MI) and improve left ventricular function and survival. We aimed to elucidate the impact of early treatment with the ACE inhibitor ramipril on the hematopoietic response after MI, as well as on the chronic systemic and vascular inflammation. Methods and Results: In a mouse model of MI, induced by permanent ligation of the left anterior descending artery, immediate initiation of treatment with ramipril (10 mg/k/d via drinking water) reduced cardiac inflammation and the number of circulating inflammatory monocytes, whereas left ventricular function was not altered significantly, respectively. This effect was accompanied by enhanced retention of hematopoietic stem cells, Lin−Sca1−c-Kit+CD34+CD16/32+ granulocyte–macrophage progenitors (GMP) and Lin−Sca1−c-Kit+CD150−CD48− multipotent progenitors (MPP) in the bone marrow, with an upregulation of the niche factors Angiopoetin 1 and Kitl at 7 d post MI. Long-term ACE inhibition for 28 d limited vascular inflammation, particularly the infiltration of Ly6Chigh monocytes/macrophages, and reduced superoxide formation, resulting in improved endothelial function in mice with ischemic heart failure. Conclusion: ACE inhibition modulates the myeloid inflammatory response after MI due to the retention of myeloid precursor cells in their bone marrow reservoir. This results in a reduction in cardiac and vascular inflammation with improvement in survival after MI.
Evaluation of Silicon and Proline Application on the Oxidative Machinery in Drought-Stressed Sugar Beet
Authors: AlKahtani Muneera D. F. , Hafez Yaser M. , Attia Kotb , Rashwan Emadeldeen , Husnain Latifa Al , AlGwaiz Hussah I. M. , Abdelaal Khaled A. A.
Published: March 2021

Drought stress deleteriously affects growth, development and productivity in plants. So, we examined the silicon effect (2 mmol) and proline (10 mmol) individually or the combination (Si + proline) in alleviating the harmful effect of drought on total phenolic compounds, reactive oxygen species (ROS), chlorophyll concentration and antioxidant enzymes as well as yield parameters of drought-stressed sugar beet plants during 2018/2019 and 2019/2020 seasons. Our findings indicated that the root diameter and length (cm), root and shoot fresh weights (g plant−1) as well as root and sugar yield significantly decreased in sugar beet plants under drought. Relative water content (RWC), nitrogen (N), phosphorus (P) and potassium (K) contents and chlorophyll (Chl) concentration considerably reduced in stressed sugar beet plants that compared with control in both seasons. Nonetheless, lipid peroxidation (MDA), electrolyte leakage (EL), hydrogen peroxide (H2O2) and superoxide (O2●−) considerably elevated as signals of drought. Drought-stressed sugar beet plants showed an increase in proline accumulation, total phenolic compounds and up-regulation of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) activity to mitigate drought effects. Si and proline individually or the combination Si + proline considerably increased root and sugar yield, sucrose%, Chl concentration and RWC, MDA and EL were remarkably reduced. The treatments led to adjust proline and total phenolic compounds as well as CAT and SOD activity in stressed sugar beet plants. We concluded that application of Si + proline under drought stress led to improve the resistance of sugar beet by regulating of proline, antioxidant enzymes, phenolic compounds and improving RWC, Chl concentration and Nitrogen, Phosphorus and Potassium (NPK) contents as well as yield parameters.
Cellular Oxidative Stress
Authors: Dossena Silvia , Marino Angela
Published: March 2021

Oxidative stress on a cellular level affects the function of tissues and organs and eventually of the whole body [...]
Protective Effects of Cannabidiol on the Membrane Proteome of UVB-Irradiated Keratinocytes
Authors: Atalay Sinemyiz , Gęgotek Agnieszka , Skrzydlewska Elżbieta
Published: March 2021

Ultraviolet (UV) radiation contained in sunlight disturbs the redox state of skin cells, leading to changes in the structures and functions of macromolecules including components of biological membranes. Cannabidiol (CBD), which accumulates in biomembranes, may be a promising protective antioxidant compound. Accordingly, the aim of this study was to compare the effects of short-term (24 h) and long-term (48 h) CBD application on the proteomic profile of biological membranes in UVB-irradiated keratinocytes. The data obtained show that UVB radiation quantitatively and qualitatively modified cell membrane proteins, with a particular research focus on adducts of proteins with the lipid peroxidation products malondialdehyde (MDA) or 4-hydroxynonenal (4-HNE). CBD application reduced the UVB-enhanced level of these protein adducts. This was particularly notable amongst proteins related to cell proliferation and apoptosis. Moreover, CBD dramatically increased the UVB-induced expression of proteins involved in the regulation of protein translation and cell proliferation (S3a/L13a/L7a ribosomal proteins), the inflammatory response (S100/S100-A6 proteins), and maintenance of redox balance (peroxiredoxin-1, carbonyl reductase 1, and aldo-keto reductase family 1 members). In contrast, CBD effects on the level of 4-HNE-protein adducts involved in the antioxidant response and proteasomal degradation process indicate that CBD may protect keratinocytes in connection with protein catabolism processes or pro-apoptotic action.
Debris Flow Characteristics in Flume Experiments Considering Berm Installation
Authors: Chang Hyungjoon , Ryou Kukhyun , Lee Hojin
Published: March 2021

This study was conducted to identify the characteristics and mobility of debris flows and analyze the performance of a berm as a debris flow mitigation measure. The debris flow velocity, flow depth, Froude number, flow resistance coefficients, and mobility ratio were accordingly determined using the results of flume tests. To analyze the influence of the berm, the results for a straight channel test without a berm were compared with those for a single-berm channel test. The debris flow velocity was observed to increase with increasing channel slope and decreasing volumetric concentration of sediment, whereas the mobility ratio was observed to increase with increasing channel slope and volumetric concentration of sediment. In addition, it was confirmed that the installation of a berm significantly decreased the debris flow velocity and mobility ratio. This indicates that a berm is an effective method for reducing damage to areas downstream of a debris flow by decreasing its potential mobility. By identifying the effects of berms on debris flow characteristics according to the channel slope and volumetric concentration of sediment, this study supports the development of berms to serve as debris flow damage mitigation measures.
Influence of Crushed Rock Properties on the Productivity of a Hydraulic Excavator
Authors: Kujundžić Trpimir , Klanfar Mario , Korman Tomislav , Briševac Zlatko
Published: March 2021

Among many factors that influence an excavator’s performance and productivity, the volume of the bucket load and duration of the excavator working cycle are crucial. In this paper, both factors were investigated, including the granulometric composition of the excavated material. The volume of material in the bucket was determined by photogrammetric analysis while the excavator cycle time was measured by analysis of video recordings captured by a digital video camera during the excavator operation. Interconnections between the angle of repose, slewing angle, particle size distribution of material, and their effects on hydraulic excavator productivity were analyzed. It was found that a larger number of fine particles in granular materials with a higher coefficient of uniformity resulted in an increase in the volume of the bucket load. Correlation analysis revealed significant interconnection between the bucket fill factor and swell factor. It was also found that calculation of the production rate according to ISO (International Organization for Standardization) standards was more accurate for materials with a higher angle of repose while the CECE (Committee for European Construction Equipment) standard was more appropriate for materials with lower angles of repose.
Finite-Horizon Kinetic Energy Optimization of a Redundant Space Manipulator
Authors: Tringali Alessandro , Cocuzza Silvio
Published: March 2021

The minimization of energy consumption is of the utmost importance in space robotics. For redundant manipulators tracking a desired end-effector trajectory, most of the proposed solutions are based on locally optimal inverse kinematics methods. On the one hand, these methods are suitable for real-time implementation; nevertheless, on the other hand, they often provide solutions quite far from the globally optimal one and, moreover, are prone to singularities. In this paper, a novel inverse kinematics method for redundant manipulators is presented, which overcomes the above mentioned issues and is suitable for real-time implementation. The proposed method is based on the optimization of the kinetic energy integral on a limited subset of future end-effector path points, making the manipulator joints to move in the direction of minimum kinetic energy. The proposed method is tested by simulation of a three degrees of freedom (DOF) planar manipulator in a number of test cases, and its performance is compared to the classical pseudoinverse solution and to a global optimal method. The proposed method outperforms the pseudoinverse-based one and proves to be able to avoid singularities. Furthermore, it provides a solution very close to the global optimal one with a much lower computational time, which is compatible for real-time implementation.
Towards the Development of an Automatic UAV-Based Indoor Environmental Monitoring System: Distributed Off-Board Control System for a Micro Aerial Vehicle
Authors: Solis Jorge , Karlsson Christoffer , Johansson Simon , Richardsson Kristoffer
Published: March 2021

This research aims to develop an automatic unmanned aerial vehicle (UAV)-based indoor environmental monitoring system for the acquisition of data at a very fine scale to detect rapid changes in environmental features of plants growing in greenhouses. Due to the complexity of the proposed research, in this paper we proposed an off-board distributed control system based on visual input for a micro aerial vehicle (MAV) able to hover, navigate, and fly to a desired target location without considerably affecting the effective flight time. Based on the experimental results, the MAV was able to land on the desired location within a radius of about 10 cm from the center point of the landing pad, with a reduction in the effective flight time of about 28%.
Correlation between Mesio-Distal Angulation and Bucco.-Lingual Inclination of First and Second Maxillary Premolars Evaluated with Panoramic Radiography and Cone-Beam Computed Tomography
Authors: Fontana Marta , Fastuca Rosamaria , Zecca Piero A. , Nucera Riccardo , Militi Angela , Lucchese Alessandra , Portelli Marco , Caprioglio Alberto
Published: March 2021

Background: Panoramic radiography creates distortion in teeth position and inclination especially in lateral areas of the arches due to acquisition and curved shape of dental arch. The aim of this study was to evaluate panoramic distortion compared to cone-beam computed tomography. Methods: Mesiodistal angulations were measured on panoramic radiography. Mesiodistal angulations and buccolingual inclinations of the teeth were determined on cone-beam computed tomography scans. The previous measurements were then compared through Pearson correlation coefficient. Results: The results showed higher correlation between mesiodistal 2D angulation and buccolingual 3D inclination, especially for first premolars, rather than between mesiodistal angulations measured in 2D and 3D. Conclusion: Especially for the lateral areas of the dental arches, root mesiodistal angulation measured on panoramic radiography is not reliable; in fact, it is greatly influenced by buccolingual inclination. Therefore, clinical evaluation is highly recommended with panoramic radiography to provide correct diagnosis and the most satisfactory results.
Fractal Dimension and Retinal Pathology: A Meta-Analysis
Authors: Yu Sam , Lakshminarayanan Vasudevan
Published: March 2021

Due to the fractal nature of retinal blood vessels, the retinal fractal dimension is a natural parameter for researchers to explore and has garnered interest as a potential diagnostic tool. This review aims to summarize the current scientific evidence regarding the relationship between fractal dimension and retinal pathology and thus assess the clinical value of retinal fractal dimension. Following the PRISMA guidelines, a literature search for research articles was conducted in several internet databases (EMBASE, MEDLINE, Web of Science, Scopus). This led to a result of 28 studies included in the final review, which were analyzed via meta-analysis to determine whether the fractal dimension changes significantly in retinal disease versus normal individuals. From the meta-analysis, summary effect sizes and 95% confidence intervals were derived for each disease category. The results for diabetic retinopathy and myopia suggest decreased retinal fractal dimension for those pathologies with the association for other diseases such as diabetes mellitus, hypertension, and glaucoma remaining uncertain. Due to heterogeneity in imaging/fractal analysis setups used between studies, it is recommended that standardized retinal fractal analysis procedures be implemented in order to facilitate future meta-analyses.
Experimental Study and Development of Design Formula for Estimating the Ultimate Strength of Curved Plates
Authors: Kim Jeong-Hyeon , Park Doo-Hwan , Kim Seul-Kee , Kim Myung-Sung , Lee Jae-Myung
Published: March 2021

The curved plate has been extensively used as a structural member in many industrial fields, especially the shipbuilding industry. The present study investigated the ultimate strength and collapse behavior of the simply supported curved plate under a longitudinal compressive load. To do this, experimental apparatuses for evaluating the buckling collapse test of the curved plates was developed. Then, a series of buckling collapse experiments was carried out by considering the flank angle, slenderness ratio, and aspect ratio of plates. To examine the fundamental buckling and collapse behavior of the curved plate, elastoplastic large deflection analysis was performed using the commercial finite element analysis program. On the basis of both the experimental and FE analysis, the effects of the flank angle, slenderness ratio, and aspect ratio on the characteristics of the buckling and collapse behavior of the curved plates are discussed. Finally, the empirical design formula for predicting the ultimate strength of curved plates was derived. The proposed empirical formula is a good indicator for estimating the behavior of the curved plate.
Dirac Equation-Based Formulation for the Quantum Conductivity in 2D-Nanomaterials
Authors: Pierantoni Luca , Pelagalli Nicola , Mencarelli Davide , Di Donato Andrea , Orlandini Matteo , Pagliuca Jacopo , Rozzi Tullio
Published: March 2021

Starting from the four component-Dirac equation for free, ballistic electrons with finite mass, driven by a constant d.c. field, we derive a basic model of scalar quantum conductivity, capable of yielding simple analytic forms, also in the presence of magnetic and polarization effects. The classical Drude conductivity is recovered as a limit case. A quantum-mechanical evaluation is provided for parabolic and linear dispersion, as in graphene, recovering currently used expressions as particular cases. Numerical values are compared with the ones from the literature in the case of graphene under d.c. applied field. In particular, the effect of the sample length and field strength on the conductivity are highlighted.
DyEgoVis: Visual Exploration of Dynamic Ego-Network Evolution
Authors: Fu Kun , Mao Tingyun , Wang Yang , Lin Daoyu , Zhang Yuanben , Sun Xian
Published: March 2021

Ego-network, which can describe relationships between a focus node (i.e., ego) and its neighbor nodes (i.e., alters), often changes over time. Exploring dynamic ego-networks can help users gain insight into how each ego interacts with and is influenced by the outside world. However, most of the existing methods do not fully consider the multilevel analysis of dynamic ego-networks, resulting in some evolution information at different granularities being ignored. In this paper, we present an interactive visualization system called DyEgoVis which allows users to explore the evolutions of dynamic ego-networks at global, local and individual levels. At the global level, DyEgoVis reduces dynamic ego-networks and their snapshots to 2D points to reveal global patterns such as clusters and outliers. At the local level, DyEgoVis projects all snapshots of the selected dynamic ego-networks onto a 2D space to identify similar or abnormal states. At the individual level, DyEgoVis utilizes a novel layout method to visualize the selected dynamic ego-network so that users can track, compare and analyze changes in the relationships between the ego and alters. Through two case studies on real datasets, we demonstrate the usability and effectiveness of DyEgoVis.