bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study on Evaluation and Application of Snowmelt Performance of Anti-Icing Asphalt Pavement
Authors: Zheng Mulian , Wu Shujuan , Wang Chongtao , Li Yifeng , Ma Zonghui , Peng Lei
Published: June 2017

This paper presents a quantitative estimation of anti-icing asphalt mixture snow-melting performance and provides the application guidance for the mixture under study. The regression model of snowmelt quality evolution was established and experimentally verified for different values of snowfall intensity, temperature and blending content of anti-icing filler. The deicing performance of anti-icing asphalt mixtures was evaluated via the impact load tests. The results obtained indicate that the application temperatures of the anti-icing asphalt mixture should exceed −10 °C for moderate or light snow conditions and exceed 0 °C for heavy or blizzard snow conditions. A linear dependence between the snowmelt quality of anti-icing asphalt mixture and time is observed for all tested values of snowfall intensity and temperature. On the other hand, under the same snowfall intensity conditions, a two- to threefold increase of the melting rate with temperature is observed. The available records for the last 30 years in different regions under study concerning variation ranges of three meteorological factors (namely, the extremely minimum temperature, snowfall intensity and the coldest month’s average temperature), the most suitable temperature for application of anti-icing asphalt pavement was identified for each particular region.
Rooftop PV Potential in the Residential Sector of the Kingdom of Saudi Arabia
Authors: Khan Mohammed , Asif Muhammad , Stach Edgar
Published: June 2017

The Kingdom of Saudi Arabia (KSA) has a fast growing building sector driven by factors like burgeoning population, economic and infrastructure development and modernization. Buildings, owing to their energy intensive operation, are imposing significant energy, environmental and economic burdens for the country. To overcome these challenges and improve the sustainability levels in the building and energy sectors, it has been decided to develop 9500 MWs of renewable energy projects by 2023. Given the annual solar radiation level of over 2200 kWh/m2, solar energy is expected to play the predominant role in achieving this target. Traditionally, KSA’s emphasis has been to pursue large-scale energy projects. Globally, however, small scale and building related projects have significantly contributed to the rapid growth of solar energy. Application of solar technologies in buildings is one of the important areas that has been ignored in the country. This paper explores the potential for rooftop solar photovoltaic (PV) in KSA’s residential sector. Taking into account appropriate PV design considerations and local building construction and cultural practices, it calculates the power generation potential for 13 major cities. It is found that a total of 51 TWh of electricity can be annually generated from these cities, accounting for 30% of the total domestic demand. Findings of a case study on application of the PV system on 248,452 m2 of rooftop area of the residential buildings in the King Fahd University of Petroleum and Minerals undertaken with the help of ArcGIS 10.2 and PVsyst modeling have also been provided.
Theoretical Analysis of Elastic, Mechanical and Phonon Properties of Wurtzite Zinc Sulfide under Pressure
Authors: Güler Melek , Güler Emre
Published: June 2017

We report for the first time the application of a mixed-type interatomic potential to determine the high-pressure elastic, mechanical, and phonon properties of wurtzite zinc sulfide (WZ-ZnS) with geometry optimization calculations under pressures up to 12 GPa. Pressure dependency of typical elastic constants, bulk, shear, and Young moduli, both longitudinal and shear wave elastic wave velocities, stability, as well as phonon dispersions and corresponding phonon density of states of WZ-ZnS were surveyed. Our results for the ground state elastic and mechanical quantities of WZ-ZnS are about experiments and better than those of some published theoretical data. Obtained phonon-related results are also satisfactory when compared with experiments and other theoretical findings.
(Li1−xFex)OHFeSe Superconductors: Crystal Growth, Structure, and Electromagnetic Properties
Authors: Zhang Guo-Yong , Chou Mitch , Lin Cheng-Tian
Published: June 2017

This review focuses on the growth of high-quality (Li1−xFex)OHFeSe single crystals by a hydrothermal method using floating-zone-grown AxFe2−ySe2 (A = K, Rb, and Cs) as precursors. The structure, superconductivity, and magnetic behavior of the obtained crystals are highly influenced by the growth conditions, such as time, temperature, and composition. A phase diagram with temperature against the c-lattice constant is summarized including the antiferromagnetic spin density wave, superconducting, and paramagnetic phases.
The Exergy Loss Distribution and the Heat Transfer Capability in Subcritical Organic Rankine Cycle
Authors: He Chao , Jiao Youzhou , Tian Chaochao , Wang Zhenfeng , Zhang Zhiping
Published: June 2017

Taking net power output as the optimization objective, the exergy loss distribution of the subcritical Organic Rankine Cycle (ORC) system by using R245fa as the working fluid was calculated under the optimal conditions. The influences of heat source temperature, the evaporator pinch point temperature difference, the expander isentropic efficiency and the cooling water temperature rise on the exergy loss distribution of subcritical ORC system are comprehensively discussed. It is found that there exists a critical value of expander isentropic efficiency and cooling water temperature rise, respectively, under certain conditions. The magnitude of critical value will affect the relative distribution of exergy loss in the expander, the evaporator and the condenser. The research results will help to better understand the characteristics of the exergy loss distribution in an ORC system.
Time-Shift Multiscale Entropy Analysis of Physiological Signals
Authors: Pham Tuan D.
Published: June 2017

Measures of predictability in physiological signals using entropy measures have been widely applied in many areas of research. Multiscale entropy expresses different levels of either approximate entropy or sample entropy by means of multiple factors for generating multiple time series, enabling the capture of more useful information than using a scalar value produced by the two entropy methods. This paper presents the use of different time shifts on various intervals of time series to discover different entropy patterns of the time series. Examples and experimental results using white noise, 1/ f noise, photoplethysmography, and electromyography signals suggest the validity and better performance of the proposed time-shift multiscale entropy analysis of physiological signals than the multiscale entropy.
Using Grounded Theory to Extend Existing PCK Framework at the Secondary Level
Authors: Martinovic Dragana , Manizade Agida
Published: June 2017

This paper addresses two critical issues in mathematics education, the need: (a) to understand the nature of educator’s subject matter knowledge and pedagogical content knowledge; and (b) to find ways to measure them. It stems from a mixed-methods study designed to inspect the secondary mathematics teachers’ pedagogical content knowledge (PCK) related to the area of a trapezoid, a common topic in intermediate/secondary school classes. Based on the provided exemplars of student work, in-service teachers were invited to propose possible ways for addressing perceived difficulties of students and provide extensions. Using a Grounded Theory approach, we identified themes in our data and incorporated them with existing conceptualizations of knowledge for teaching secondary level mathematics, and developed rubrics that allow discriminating different levels of teachers’ PCK. In this paper, we describe the process of developing the rubrics, and propose ways to: (a) extend the existing frameworks for PCK in/for teaching mathematics at the secondary level; and (b) measure multiple facets of PCK in order to design technology-based professional development for mathematics teachers.
Offshore Facilities to Produce Hydrogen
Authors: Blanco-Fernández Pilar , Pérez-Arribas Francisco
Published: June 2017

As a result of international agreements on the reduction of CO2 emissions, new technologies using hydrogen are being developed. Hydrogen, despite being the most abundant element in Nature, cannot be found in its pure state. Water is one of the most abundant sources of hydrogen on the planet. The proposal here is to use energy from the sea in order to obtain hydrogen from water. If plants to obtain hydrogen were to be placed in the ocean, the impact of long submarines piping to the coast will be reduced. Further, this will open the way for the development of ships propelled by hydrogen. This paper discusses the feasibility of an offshore installation to obtain hydrogen from the sea, using ocean wave energy.
Compact Narrow Linewidth Actively Q-Switched Er–Yb Double-Clad Fiber Laser
Authors: Posada-Ramírez Berenice , Durán-Sánchez Manuel , Alvarez-Tamayo Ricardo I. , Alaniz-Baylón Jared , Salceda-Delgado Guillermo , Kuzin Evgeny A. , Ibarra-Escamilla Baldemar
Published: June 2017

Actively Q-switched laser operation of a narrow linewidth compact fiber laser based on an Er–Yb double-clad fiber is presented. The laser linewidth as a function of the repetition rate and the Q-switched pulses characteristics for different pump powers are experimentally analyzed. Stable Q-switched laser operation with spectral laser linewidth of 73 pm in a repetition rate range from 90 to 270 kHz is obtained. The minimum pulse duration of 178 ns, maximum peak power of 30.5 W, and maximum pulse energy of 5.4 µJ are observed. The maximum average power reached is 1.1 W.
Impact of Sweet Potato Starch-Based Nanocomposite Films Activated With Thyme Essential Oil on the Shelf-Life of Baby Spinach Leaves
Authors: Issa Aseel , Ibrahim Salam A. , Tahergorabi Reza
Published: June 2017

Salmonella Typhimurium (S. Typhi) and Escherichia coli (E. coli) have been responsible for an increasing number of outbreaks linked to fresh produce, such as baby spinach leaves, in the last two decades. More recently, antimicrobial biodegradable packaging systems have been attracting much attention in the food packaging industry as eco-friendly alternatives to conventional plastic packaging. The objective of this study was to evaluate the effect of antibacterial nanocomposite films on inoculated spinach leaves and on the sensory properties of these leaves during eight days of refrigerated storage. In this study, an antibacterial film comprised of sweet potato starch (SPS), montmorillonite (MMT) nanoclays and thyme essential oil (TEO) as a natural antimicrobial agent was developed. Our results showed that the incorporation of TEO in the film significantly (p < 0.05) reduced the population of E. coli and S. Typhi on fresh baby spinach leaves to below detectable levels within five days, whereas the control samples without essential oil maintained approximately 4.5 Log colony forming unit (CFU)/g. The sensory scores for spinach samples wrapped in films containing TEO were higher than those of the control. This study thus suggests that TEO has the potential to be directly incorporated into a SPS film to prepare antimicrobial nanocomposite films for food packaging applications.
Temperature-Triggered Colloidal Gelation through Well-Defined Grafted Polymeric Surfaces
Authors: Van Doorn Jan Maarten , Sprakel Joris , Kodger Thomas E.
Published: June 2017

Sufficiently strong interparticle attractions can lead to aggregation of a colloidal suspension and, at high enough volume fractions, form a mechanically rigid percolating network known as a colloidal gel. We synthesize a model thermo-responsive colloidal system for systematically studying the effect of surface properties, grafting density and chain length, on the particle dynamics within colloidal gels. After inducing an attraction between particles by heating, aggregates undergo thermal fluctuation which we observe and analyze microscopically; the magnitude of the variance in bond angle is larger for lower grafting densities. Macroscopically, a clear increase of the linear mechanical behavior of the gels on both the grafting density and chain length arises, as measured by rheology, which is inversely proportional to the magnitude of local bond angle fluctuations. This colloidal system will allow for further elucidation of the microscopic origins to the complex macroscopic mechanical behavior of colloidal gels including bending modes within the network.
Measuring Neighborhood Walkable Environments: A Comparison of Three Approaches
Authors: Chiang Yen-Cheng , Sullivan William , Larsen Linda
Published: June 2017

Multiple studies have revealed the impact of walkable environments on physical activity. Scholars attach considerable importance to leisure and health-related walking. Recent studies have used Google Street View as an instrument to assess city streets and walkable environments; however, no study has compared the validity of Google Street View assessments of walkable environment attributes to assessments made by local residents and compiled from field visits. In this study, we involved nearby residents and compared the extent to which Google Street View assessments of the walkable environment correlated with assessments from local residents and with field visits. We determined the assessment approaches (local resident or field visit assessments) that exhibited the highest agreement with Google Street View. One city with relatively high-quality walkable environments and one city with relatively low-quality walkable environments were examined, and three neighborhoods from each city were surveyed. Participants in each neighborhood used one of three approaches to assess the walkability of the environment: 15 local residents assessed the environment using a map, 15 participants made a field visit to assess the environment, and 15 participants used Google Street View to assess the environment, yielding a total of 90 valid samples for the two cities. Findings revealed that the three approaches to assessing neighborhood walkability were highly correlated for traffic safety, aesthetics, sidewalk quality, and physical barriers. Compared with assessments from participants making field visits, assessments by local residents were more highly correlated with Google Street View assessments. Google Street View provides a more convenient, low-cost, efficient, and safe approach to assess neighborhood walkability. The results of this study may facilitate future large-scale walkable environment surveys, effectively reduce expenses, and improve survey efficiency.
Glyphosate Residues in Groundwater, Drinking Water and Urine of Subsistence Farmers from Intensive Agriculture Localities: A Survey in Hopelchén, Campeche, Mexico
Authors: Rendon-von Osten Jaime , Dzul-Caamal Ricardo
Published: June 2017

The use of pesticides in Mexican agriculture creates an interest in learning about the presence of these substances in different environmental matrices. Glyphosate (GLY) is an herbicide widely used in the state of Campeche, located in the Mayan zone in the western Yucatan peninsula. Despite the fact that GLY is considered a non-toxic pesticide to humans, its presence in water bodies through spillage, runoff, and leaching are a risk to human health or biota that inhabit these ecosystems. In the present study, glyphosate residues were determined in groundwater, bottled drinking water, and the urine of subsistence farmers from various localities of the Hopelchén municipality in Campeche. Determination of GLY was carried out using Enzyme-Linked Immunosorbent Assay (ELISA). The highest concentrations of GLY were observed in the groundwater (1.42 μg/L) of Ich-Ek and urine (0.47 μg/L) samples of subsistence farmers from the Francisco J. Mújica communities. The glyphosate concentrations in groundwater and bottled drinking water indicate an exposure and excessive use of glyphosate in these agricultural communities. This is one of the first studies that reports glyphosate concentration levels in human urine and bottled drinking water in México and in the groundwater in the Yucatan Peninsula as part of a prospective pilot study, to which a follow-up will be performed to monitor this trend over time.
Associations among Screen Time and Unhealthy Behaviors, Academic Performance, and Well-Being in Chinese Adolescents
Authors: Yan Hanyi , Zhang Rui , Oniffrey Theresa , Chen Guoxun , Wang Yueqiao , Wu Yingru , Zhang Xinge , Wang Quan , Ma Lu , Li Rui , Moore Justin
Published: June 2017

Screen time is negatively associated with markers of health in western youth, but very little is known about these relationships in Chinese youth. Middle-school and high-school students (n = 2625) in Wuhan, China, completed questionnaires assessing demographics, health behaviors, and self-perceptions in spring/summer 2016. Linear and logistic regression analyses were conducted to determine whether, after adjustment for covariates, screen time was associated with body mass index (BMI), eating behaviors, average nightly hours of sleep, physical activity (PA), academic performance, and psychological states. Watching television on school days was negatively associated with academic performance, PA, anxiety, and life satisfaction. Television viewing on non-school days was positively associated with sleep duration. Playing electronic games was positively associated with snacking at night and less frequently eating breakfast, and negatively associated with sleep duration and self-esteem. Receiving electronic news and study materials on non-school days was negatively associated with PA, but on school days, was positively associated with anxiety. Using social networking sites was negatively associated with academic performance, but positively associated with BMI z-score, PA and anxiety. Screen time in adolescents is associated with unhealthy behaviors and undesirable psychological states that can contribute to poor quality of life.
Identifying Factors Associated with Risk Assessment Competencies of Public Health Emergency Responders
Authors: Hao Jiejing , Ren Jiaojiao , Wu Qunhong , Hao Yanhua , Sun Hong , Ning Ning , Ding Ding
Published: June 2017

This study aimed to better understand the current situation of risk assessment and identify the factors associated with competence of emergency responders in public health risk assessment. The participants were selected by a multi-stage, stratified cluster sampling method in Heilongjiang Centers for Disease Control and Prevention (CDC). The questionnaires that measured their perceptions on risk assessment competences were administered through the face-to-face survey. A final sample of 1889 staff was obtained. Of this sample, 78.6% of respondents rated their own risk assessment competences as “relatively low”, contrasting with 21.4% rated as “relatively high”. Most of the respondents (62.7%) did not participate in any risk assessment work. Only 13.7% and 42.7% of respondents reported participating in risk assessment training and were familiar with risk assessment tools. There existed statistical significance between risk assessment-related characteristics of respondents and their self-rated competences scores. Financial support from the government and administrative attention were regarded as the important factors contributing to risk assessment competences of CDC responders. Higher attention should be given to risk assessment training and enhancing the availability of surveillance data. Continuous efforts should be made to remove the financial and technical obstacles to improve the competences of risk assessment for public health emergency responders.
Trends in Pediatric and Adolescent Anterior Cruciate Ligament Injuries in Victoria, Australia 2005–2015
Authors: Shaw Louise , Finch Caroline
Published: June 2017

Anterior cruciate ligament (ACL) injuries in children and adolescents have been the focus of recent media attention and parental concern, given their potential for adverse long-term health outcomes and healthcare costs. However, there is limited formal evidence on trends in the incidence of ACL injuries in children. This study utilizes the Victorian Admitted Episodes Dataset (VAED) to characterize epidemiologic trends of hospital-admitted ACL injuries in those aged 5 to 14 years over a period of 10 years from 2005 to 2015. There was a total of 320 cases and the overall annual rate of ACL injuries increased by 147.8% from 2.74 per 100,000 population in 2005/2006 to 6.79 per 100,000 in 2014/2015. The majority (96.9%) of these injuries were in 10- to 14-year-olds. The main in-hospital procedure provided to over 80% of the hospitalized cases involved ACL reconstruction. Sporting activities accounted for 56.6% of ACL injuries. For females, over half (52.4%) of ACL injuries occurred whilst playing ball sports, compared to 35.4% of males. The large increase in ACL injuries in 5- to 14-year-olds in the state of Victoria, Australia over a 10-year period indicates they are a significant and emerging health burden. Population-wide ACL prevention policies are required to halt these trends. Cost effective prevention programs that involve neuromuscular training must be implemented in schools and junior sports teams.
Quality of Life of Post-Mastectomy Women Living in a Semi-Arid Region of Brazil
Authors: Araújo Neto Emídio , Alves Beatriz , Gehrke Flávia , Azzalis Ligia , Junqueira Virginia , Sousa Luiz , Adami Fernando , Fonseca Fernando
Published: June 2017

Health is the major reference regarding quality of life; when it comes to breast cancer in particular, the loss of a breast traumatically affects a woman’s life, reflecting on her quality of life. Recognizing this problem, our aim was to investigate the quality of life of women who live in a semi-arid region of Brazil after breast cancer mastectomy. In this exploratory, transversal and observational study, a Brazilian variantof the shorter version of the original instrument from the World Health Organization Quality of Life (WHOQOL-BREF), applied in the study population, was analyzed and their socio-demographic profile was obtained. The sample was composed of 50 mastectomized women. The 50 included patients comprised women at a mean age of 54 years. Most of them had finished elementary school, and their average income was one Brazilian minimum monthly wage. Regarding the data related to quality of life, the highest score was found in the social relationships domain (4.29) followed by the psychological (4.09) and environmental (3.88) domains. The lowest score observed was for the physical domain (3.48). With these findings we can say that social and psychological parameters are driving factors of the quality of life in post-mastectomy women. Therefore, these results are useful to establish strategies to improve the quality of life of breast cancer mastectomy patients.
Understanding the Challenges of Improving Sanitation and Hygiene Outcomes in a Community Based Intervention: A Cross-Sectional Study in Rural Tanzania
Authors: Kamara Joseph , Galukande Moses , Maeda Florence , Luboga Sam , Renzaho Andre
Published: June 2017

Good sanitation and clean water are basic human rights yet they remain elusive to many rural communities in Sub-Saharan Africa (SSA). We carried out a cross sectional study to examine the impact of a four-year intervention aimed at improving access to water and sanitation and reducing waterborne disease, especially diarrhea in children under five years old. The study was carried out in April and May 2015 in Busangi, Chela and Ntobo wards of Kahama District of Tanzania. The interventions included education campaigns and improved water supply, and sanitation. The percentage of households (HHs) with access to water within 30 min increased from 19.2 to 48.9 and 17.6 to 27.3 in the wet and dry seasons, respectively. The percentage of HHs with hand washing facilities at the latrine increased from 0% to 13.2%. However, the incidence of diarrhea among children under five years increased over the intervention period, RR 2.91 95% CI 2.71–3.11, p < 0.0001. Availability of water alone may not influence the incidence of waterborne diseases. Factors such as water storage and usage, safe excreta disposal and other hygiene practices are critical for interventions negating the spread of water borne diseases. A model that articulates the extent to which these factors are helpful for such interventions should be explored.
Seroprevalence of Measles Antibodies and Predictors for Seropositivity among Chinese Children
Authors: Wang Xiaoqin , Ma Mei , Hui Zhaozhao , Terry Paul , Zhang Yue , Su Rui , Wang Mingxu , Gu Wei , Li Ling
Published: June 2017

Background: Supplementary measles immunization has been implemented since 2010 throughout China, yet few studies have reported its effect in the northwest regions. Methods: A cross-sectional study was conducted among children aged 2 to 4 years old (n = 755) from February to September 2014 in 25 towns of Qian County, Shaanxi Province. Blood samples were analyzed for measles antibodies using enzyme-linked immunosorbent immunoglobulin G (IgG) assays. Socio-demographic factors were assessed by questionnaire. Data on vaccine dose were collected from town medical records. Univariate and logistic regression analyses were used to determine factors associated with measles antibody seropositivity. Results: Measles antibody seroprevalence was 91.13% (95% CI: 89.52–92.83) in our sample. Compared with children whose mother’s highest education was primary school, seroprevalence was higher in children whose maternal education was middle school (adjusted OR: 1.4, 95% CI: 0.7–2.8), high school (adjusted OR: 2.4, 95% CI: 1.3–7.7), and college/university (adjusted OR: 2.9, 95% CI: 1.2–9.3). Vaccine dose was positively associated with seropositivity. Conclusions: Measles seroprevalence is high in China and is associated with the mother’s education and vaccine dose.
A High Performance, Spatiotemporal Statistical Analysis System Based on a Spatiotemporal Cloud Platform
Authors: Jin Baoxuan , Song Weiwei , Zhao Kang , Wei Xiaoyan , Hu Fei , Jiang Yongyao
Published: June 2017

With the increase in size and complexity of spatiotemporal data, traditional methods for performing statistical analysis are insufficient for meeting real-time requirements for mining information from Big Data, due to both data- and computing-intensive factors. To solve the Big Data challenges in geostatistics and to support decision-making, a high performance, spatiotemporal statistical analysis system (Geostatistics-Hadoop) is proposed in this paper. The proposed system has several features: (1) Hadoop is enhanced to handle spatial data in a native format and execute a number of parallelized spatial analysis algorithms to solve practical geospatial analysis problems; (2) the Oozie-based workflow system is utilized to ease the operation and sharing of spatial analysis services; and (3) a private cloud platform based on Eucalyptus is leveraged to provide on-the-fly and elastic computing resources. Experimental results show that Geostatistics-Hadoop efficiently conducts rapid information mining and analysis of big spatiotemporal data sets, with the support of elastic computing resources from a cloud platform. The adoption of cloud computing and the Hadoop cluster to parallelize statistical calculations significantly improves the performance of Big Data analyses.
Resveratrol, 4′ Acetoxy Resveratrol, R-equol, Racemic Equol or S-equol as Cosmeceuticals to Improve Dermal Health
Authors: Lephart Edwin D.
Published: June 2017

Phytochemicals are botanical compounds used in dermatology applications as cosmeceuticals to improve skin health. Resveratrol and equol are two of the best-known polyphenolic or phytoestrogens having similar chemical structures and some overlapping biological functions to 17β-estradiol. Human skin gene expression was reviewed for 28 different biomarkers when resveratrol, 4′ acetoxy resveratrol (4AR), R-equol, racemic equol or S-equol were tested. Sirtuin 1 activator (SIRT 1) was stimulated by resveratrol and 4AR only. Resveratrol, R-equol and racemic equol were effective on the aging biomarkers proliferating cell nuclear factor (PCNA), nerve growth factor (NGF), 5α-reductase and the calcium binding proteins S100 A8 and A9. Racemic equol and 4AR displayed among the highest levels for the collagens, elastin and tissue inhibitor of the matrix metalloproteinase 1 (TIMP 1). S-equol displayed the lowest level of effectiveness compared to the other compounds. The 4AR analog was more effective compared to resveratrol by 1.6-fold. R-equol and racemic equol were almost equal in potency displaying greater inhibition vs. resveratrol or its 4′ analog for the matrix metalloproteinases (MMPs), but among the inflammatory biomarkers, resveratrol, 4AR, R-equol and racemic equol displayed high inhibition. Thus, these cosmeceuticals display promise to improve dermal health; however, further study is warranted to understand how phytochemicals protect/enhance the skin.
Photoreceptor PhyB Involved in Arabidopsis Temperature Perception and Heat-Tolerance Formation
Authors: Song Junyi , Liu Qijun , Hu Biru , Wu Wenjian
Published: June 2017

The influence of temperature on plants is essential. However, our knowledge on the intricate regulation process underlying heat stress (HS) response in plants is limited. Recently, information about thermal sensors in vivo has begun to emerge. In this study, another primary environmental stimulus, light, was verified once again to work with temperature synergistically on plants, through the modulation of numerous biological processes. With the application of transcriptomic analysis, a substantial number of heat-responsive genes were detected involved in both light- and phytohormone-mediated pathways in Arabidopsis. During this process, phytoreceptor phyB acts as a molecular switch to turn on or turn off several other genes HS response, under different light conditions. Furthermore, a morphological study showed the afunction of phyB enhanced plants thermal tolerance, confirming the important role of this phytochrome in temperature perception and response in plants. This study adds data to the picture of light and temperature signaling cross-talk in plants, which is important for the exploration of complicated HS responses or light-mediated mechanisms. Furthermore, based on its influence on Arabidopsis thermal response in both morphological and physiological levels, phyB is a photoreceptor, as revealed before, as well as an essential thermal sensor in plants.
Biochemical and Computational Insights on a Novel Acid-Resistant and Thermal-Stable Glucose 1-Dehydrogenase
Authors: Ding Haitao , Gao Fen , Yu Yong , Chen Bo
Published: June 2017

Due to the dual cofactor specificity, glucose 1-dehydrogenase (GDH) has been considered as a promising alternative for coenzyme regeneration in biocatalysis. To mine for potential GDHs for practical applications, several genes encoding for GDH had been heterogeneously expressed in Escherichia coli BL21 (DE3) for primary screening. Of all the candidates, GDH from Bacillus sp. ZJ (BzGDH) was one of the most robust enzymes. BzGDH was then purified to homogeneity by immobilized metal affinity chromatography and characterized biochemically. It displayed maximum activity at 45 °C and pH 9.0, and was stable at temperatures below 50 °C. BzGDH also exhibited a broad pH stability, especially in the acidic region, which could maintain around 80% of its initial activity at the pH range of 4.0–8.5 after incubating for 1 hour. Molecular dynamics simulation was conducted for better understanding the stability feature of BzGDH against the structural context. The in-silico simulation shows that BzGDH is stable and can maintain its overall structure against heat during the simulation at 323 K, which is consistent with the biochemical studies. In brief, the robust stability of BzGDH made it an attractive participant for cofactor regeneration on practical applications, especially for the catalysis implemented in acidic pH and high temperature.
Insulin Treatment May Alter Fatty Acid Carriers in Placentas from Gestational Diabetes Subjects
Authors: Ruiz-Palacios Maria , Prieto-Sánchez Maria , Ruiz-Alcaraz Antonio , Blanco-Carnero José , Sanchez-Campillo Maria , Parrilla Juan , Larqué Elvira
Published: June 2017

There is little information available on the effect of Gestational diabetes mellitus (GDM) treatment (diet or insulin) on placental lipid carriers, which may influence fetal fat accretion. Insulin may activate placental insulin receptors protein kinase (AKT) and extracellular signal regulated kinase ERK mediators, which might affect lipid metabolism. Placenta was collected from 25 control women, 23 GDM-Diet and 20 GDM-Insulin. Western blotting of insulin signaling mediators and lipid carriers was performed. The human choricarcinoma-derived cell line BeWo was preincubated with insulin inhibitors protein kinase (AKT) and extracellular signal regulated kinase (ERK) and ERK inhibitors to evaluate insulin regulation of lipid carriers. Maternal serum insulin at recruitment correlated to ultrasound fetal abdominal circumference in offspring of GDM and placental endothelial lipase (EL). Lipoprotein lipase in placenta was significantly reduced in both GDM, while most of the other lipid carriers tended to higher values, although not significantly. There was a significant increase in both phosphorylated-Akt and ERK in placentas from GDM-Insulin patients; both were associated to placental fatty acid translocase (FAT), fatty acid binding protein (A-FABP), and EL. BeWo cells treated with insulin pathway inhibitors significantly reduced A-FABP, fatty acid transport protein (FATP-1), and EL levels, confirming the role of insulin on these carriers. We conclude that insulin promotes the phosphorylation of placental insulin mediators contributing to higher levels of some specific fatty acid carriers in the placenta and fetal adiposity in GDM.
Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel?
Authors: Rayner Tracey , Moreau Carol , Ambrose Mike , Isaac Peter , Ellis Noel , Domoney Claire
Published: June 2017

One of the traits studied by Mendel in pea (Pisum sativum L.) was the wrinkled-seeded phenotype, and the molecular basis for a mutation underlying this phenotype was discovered in the 1990s. Although the starch-branching enzyme gene mutation identified at the genetic locus r is most likely to be that in seeds available to Mendel in the mid-1800s, it has remained an open question as to whether or not additional natural mutations in this gene exist within Pisum germplasm collections. Here, we explore this question and show that all but two wrinkled-seeded variants in one such collection correspond to either the mutant allele described previously for the r locus or a mutation at a second genetic locus, rb, affecting the gene encoding the large subunit of Adenosine diphosphoglucose (ADP-glucose) pyrophosphorylase; the molecular basis for the rb mutation is described here. The genetic basis for the phenotype of one (JI 2110) of the two lines which are neither r nor rb has been studied in crosses with a round-seeded variant (JI 281); for which extensive genetic marker data were expected. In marked contrast to the trait studied by Mendel and the rb phenotype; the data suggest that the wrinkled-seeded phenotype in JI 2110 is maternally determined, controlled by two genetic loci, and the extent to which it is manifested is very sensitive to the environment. Metabolite analysis of the cotyledons of JI 2110 revealed a profile for sucrose and sucrose-derived compounds that was more similar to that of wild-type round-seeded, than that of wrinkled-seeded r, pea lines. However, the metabolite profile of the seed coat (testa) of JI 2110 was distinct from that of other round-seeded genotypes tested which, together with analysis of recombinant inbred progeny lines, suggests an explanation for the seed phenotype.
Cellular Morphology-Mediated Proliferation and Drug Sensitivity of Breast Cancer Cells
Authors: Domura Ryota , Sasaki Rie , Ishikawa Yuma , Okamoto Masami
Published: June 2017

The interpretation of the local microenvironment of the extracellular matrix for malignant tumor cells is in intimate relation with metastatic spread of cancer cells involving the associated issues of cellular proliferation and drug responsiveness. This study was aimed to assess the combination of both surface topographies (fiber alignments) and different stiffness of the polymeric substrates (poly(l-lactic acid) and poly(ε-caprolactone), PLLA and PCL, respectively) as well as collagen substrates (coat and gel) to elucidate the effect of the cellular morphology on cellular proliferation and drug sensitivities of two different types of breast cancer cells (MDA-MB-231 and MCF-7). The morphological spreading parameter (nucleus/cytoplasm area ratio) induced by the anthropogenic substrates has correlated intimately with the cellular proliferation and the drug sensitivity the half maximal inhibitory concentration (IC50) of cancer cells. This study demonstrated the promising results of the parameter for the evaluation of cancer cell malignancy.
A Multi-Projector Calibration Method for Virtual Reality Simulators with Analytically Defined Screens
Authors: Portalés Cristina , Casas Sergio , Coma Inmaculada , Fernández Marcos
Published: June 2017

The geometric calibration of projectors is a demanding task, particularly for the industry of virtual reality simulators. Different methods have been developed during the last decades to retrieve the intrinsic and extrinsic parameters of projectors, most of them being based on planar homographies and some requiring an extended calibration process. The aim of our research work is to design a fast and user-friendly method to provide multi-projector calibration on analytically defined screens, where a sample is shown for a virtual reality Formula 1 simulator that has a cylindrical screen. The proposed method results from the combination of surveying, photogrammetry and image processing approaches, and has been designed by considering the spatial restrictions of virtual reality simulators. The method has been validated from a mathematical point of view, and the complete system—which is currently installed in a shopping mall in Spain—has been tested by different users.
Correction: Hajieghrary, H.; Mox, D.; Hsieh, M.A. Information Theoretic Source Seeking Strategies for Multiagent Plume Tracking in Turbulent Fields. Journal of Marine Science and Engineering 2017, 5, 3
Authors: Hajieghrary Hadi , Mox Daniel , Hsieh M.
Published: June 2017

The authors wish to correct the Acknowledgments section in their paper [1] as follows: [...]
Frequency-Stable Ionic-Type Hybrid Gate Dielectrics for High Mobility Solution-Processed Metal-Oxide Thin-Film Transistors
Authors: Heo Jae , Choi Seungbeom , Jo Jeong-Wan , Kang Jingu , Park Ho-Hyun , Kim Yong-Hoon , Park Sung
Published: June 2017

In this paper, we demonstrate high mobility solution-processed metal-oxide thin-film transistors (TFTs) by using a high-frequency-stable ionic-type hybrid gate dielectric (HGD). The HGD gate dielectric, a blend of sol-gel aluminum oxide (AlOx) and poly(4-vinylphenol) (PVP), exhibited high dielectric constant (ε~8.15) and high-frequency-stable characteristics (1 MHz). Using the ionic-type HGD as a gate dielectric layer, an minimal electron-double-layer (EDL) can be formed at the gate dielectric/InOx interface, enhancing the field-effect mobility of the TFTs. Particularly, using the ionic-type HGD gate dielectrics annealed at 350 °C, InOx TFTs having an average field-effect mobility of 16.1 cm2/Vs were achieved (maximum mobility of 24 cm2/Vs). Furthermore, the ionic-type HGD gate dielectrics can be processed at a low temperature of 150 °C, which may enable their applications in low-thermal-budget plastic and elastomeric substrates. In addition, we systematically studied the operational stability of the InOx TFTs using the HGD gate dielectric, and it was observed that the HGD gate dielectric effectively suppressed the negative threshold voltage shift during the negative-illumination-bias stress possibly owing to the recombination of hole carriers injected in the gate dielectric with the negatively charged ionic species in the HGD gate dielectric.
Hydrogen-Induced Delayed Cracking in TRIP-Aided Lean-Alloyed Ferritic-Austenitic Stainless Steels
Authors: Papula Suvi , Sarikka Teemu , Anttila Severi , Talonen Juho , Virkkunen Iikka , Hänninen Hannu
Published: June 2017

Susceptibility of three lean-alloyed ferritic-austenitic stainless steels to hydrogen-induced delayed cracking was examined, concentrating on internal hydrogen contained in the materials after production operations. The aim was to study the role of strain-induced austenite to martensite transformation in the delayed cracking susceptibility. According to the conducted deep drawing tests and constant load tensile testing, the studied materials seem not to be particularly susceptible to delayed cracking. Delayed cracks were only occasionally initiated in two of the materials at high local stress levels. However, if a delayed crack initiated in a highly stressed location, strain-induced martensite transformation decreased the crack arrest tendency of the austenite phase in a duplex microstructure. According to electron microscopy examination and electron backscattering diffraction analysis, the fracture mode was predominantly cleavage, and cracks propagated along the body-centered cubic (BCC) phases ferrite and α’-martensite. The BCC crystal structure enables fast diffusion of hydrogen to the crack tip area. No delayed cracking was observed in the stainless steel that had high austenite stability. Thus, it can be concluded that the presence of α’-martensite increases the hydrogen-induced cracking susceptibility.
Non-Destructive Inspection of Impact Damage in Composite Aircraft Panels by Ultrasonic Guided Waves and Statistical Processing
Authors: Capriotti Margherita , Kim Hyungsuk E. , Scalea Francesco Lanza di , Kim Hyonny
Published: June 2017

This paper discusses a non-destructive evaluation (NDE) technique for the detection of damage in composite aircraft structures following high energy wide area blunt impact (HEWABI) from ground service equipment (GSE), such as heavy cargo loaders and other heavy equipment. The test structures typically include skin, co-cured stringers, and C-frames that are bolt-connected onto the skin with shear ties. The inspection exploits the waveguide geometry of these structures by utilizing ultrasonic guided waves and a line scan approach. Both a contact prototype and a non-contact prototype were developed and tested on realistic test panels subjected to impact in the laboratory. The results are presented in terms of receiver operating characteristic curves that show excellent probability of detection with low false alarm rates for defects located in the panel skin and stringers.
Interpreting Microbial Biosynthesis in the Genomic Age: Biological and Practical Considerations
Authors: Miller Ian , Chevrette Marc , Kwan Jason
Published: June 2017

Genome mining has become an increasingly powerful, scalable, and economically accessible tool for the study of natural product biosynthesis and drug discovery. However, there remain important biological and practical problems that can complicate or obscure biosynthetic analysis in genomic and metagenomic sequencing projects. Here, we focus on limitations of available technology as well as computational and experimental strategies to overcome them. We review the unique challenges and approaches in the study of symbiotic and uncultured systems, as well as those associated with biosynthetic gene cluster (BGC) assembly and product prediction. Finally, to explore sequencing parameters that affect the recovery and contiguity of large and repetitive BGCs assembled de novo, we simulate Illumina and PacBio sequencing of the Salinispora tropica genome focusing on assembly of the salinilactam (slm) BGC.
Chemical Profiling and Evaluation of Antioxidant and Anti-Microbial Properties of Selected Commercial Essential Oils: A Comparative Study
Authors: Luís Ângelo , Duarte Ana , Pereira Luísa , Domingues Fernanda
Published: June 2017

Background: The last decades have seen an increased awareness by the scientific community of the extent of resistance to conventional antibiotics, particularly with respect to the emerging multidrug-resistant pathogenic microbes. Additionally, natural antioxidants have received significant attention among food professionals and consumers because of their assumed safety and potential therapeutic value. The aim of this work was to assess the antioxidant activities of eight selected commercial essential oils (EOs), together with the evaluation of their antibacterial and anti-quorum sensing properties. Methods: The chemical profiling of the EOs was performed using gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant properties of the EOs were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and by β-carotene bleaching test. Disc diffusion assays were employed to evaluate the anti-bacterial and anti-quorum sensing activities of the EOs. Results: It was observed that EOs from three Eucalyptus species are rich in eucalyptol. Generally, linalool is abundant in EOs from four Lavandula species. The oil of Cymbopogon citratus is the one with the best capacity to scavenge the DPPH free radicals and presented great antibacterial activity. Conclusions: The geographical origins of the plant species are determinant factors in the EO composition and in the corresponding biological activities.
Influence of Membrane Equivalent Weight and Reinforcement on Ionic Species Crossover in All-Vanadium Redox Flow Batteries
Authors: Ashraf Gandomi Yasser , Aaron Doug , Mench Matthew
Published: June 2017

One of the major sources of lost capacity in all-vanadium redox flow batteries (VRFBs) is the undesired transport (usually called crossover) of water and vanadium ions through the ion-exchange membrane. In this work, an experimental assessment of the impact of ion-exchange membrane properties on vanadium ion crossover and capacity decay of VRFBs has been performed. Two types of cationic membranes (non-reinforced and reinforced) with three equivalent weights of 800, 950 and 1100 g·mol−1 were investigated via a series of in situ performance and capacity decay tests along with ex situ vanadium crossover measurement and membrane characterization. For non-reinforced membranes, increasing the equivalent weight (EW) from 950 to 1100 g·mol−1 decreases the V(IV) permeability by ~30%, but increases the area-specific resistance (ASR) by ~16%. This increase in ASR and decrease in V(IV) permeability was accompanied by increased through-plane membrane swelling. Comparing the non-reinforced with reinforced membranes, membrane reinforcement increases ASR, but V(IV) permeability decreases. It was also shown that there exists a monotonic correlation between the discharge capacity decay over long-term cycling and V(IV) permeability values. Thus, V(IV) permeability is considered a representative diagnostic for assessing the overall performance of a particular ion-exchange membrane with respect to capacity fade in a VRFB.
Fabrication of Functional Plastic Parts Using Nanostructured Steel Mold Inserts
Authors: Blondiaux Nicolas , Pugin Raphaël , Andreatta Gaëlle , Tenchine Lionel , Dessors Stéphane , Chauvy Pierre-François , Diserens Matthieu , Vuillermoz Philippe
Published: June 2017

We report on the fabrication of sub-micro and nanostructured steel mold inserts for the replication of nanostructured immunoassay biochips. Planar and microstructured stainless steel inserts were textured at the sub-micron and nanoscale by combining nanosphere lithography and electrochemical etching. This allowed the fabrication of structures with lateral dimensions of hundreds of nanometers and aspect ratios of up to 1:2. Nanostructured plastic parts were produced by means of hot embossing and injection molding. Surface nanostructuring was used to control wettability and increase the sensitivity of an immunoassay.