bluuebunny/crossref_metadata_2025 huggingface dataset:
Hymen ‘repair’: Views from feminists, medical professionals and the women involved in the middle east, North Africa and Europe
Authors: Saharso Sawitri
Published: January 2022

In Europe, hymen ‘repair’ is controversial because it is often seen as a concession to immigrant groups that do not respect women’s sexual autonomy. But how is hymen ‘repair’ viewed in societies in which the norm is that women should not have premarital sex? And why do women want hymen ‘repair’? Hymen ‘repair’ is also controversial in Middle Eastern and North African (MENA) countries because it is seen as undermining social mores about women and premarital sex. However, some Islamic leaders have defended the procedure. Women request hymen ‘repairs’ for a variety of reasons. Some have been sexually abused and may desire the surgery to overcome trauma. Some have had consensual sex and may fear sanctions, while others may see the surgery as a covert act of rebellion against the virginity rule. Still others may choose it to please their future husband. Hymen ‘repair’ is extensively discussed in MENA countries and in Europe. Feminists in MENA countries are divided over whether the surgery promotes sexual autonomy while, in the European debate, an important issue is whether the choice itself is an autonomous one that doctors should respect. Inspired by a relational approach to autonomy, I see the women involved as individuals with culturally informed identities and interests who may feel pressure to get the surgery yet are still capable of autonomy. I argue for a policy to stimulate debate in communities about the virginity norm and to make hymen ‘repair’ available to women. However, it should be combined with an attitude of sympathetic distrust, recognising that hymen ‘repair’ harms women’s dignity and authenticity.
Relating to the end of life through advance care planning: Expectations and experiences of people with dementia and their family caregivers
Authors: Lemos Dekker Natashe , Bolt Sascha R
Published: January 2022

Background

Dementia is widely considered a progressive condition associated with changes in cognitive capacities, which promotes the idea that people with dementia need to anticipate end-of-life care preferences. There is a growing body of interventions meant to support advance care planning (ACP) for people with dementia and their families. However, a deeper understanding of their experiences and expectations regarding planning the end of life is needed to inform ethically sound and person-centered ACP. This study explores how end-of-life care planning for people with dementia is experienced and perceived in practice.

Methods

We conducted a secondary analysis of qualitative data from in-depth interviews and ethnographic fieldwork. Participants included people with dementia living in the community or in nursing homes, family caregivers, and professional caregivers. During reflective sessions, the researchers moved back and forth between their original data and overarching topics related to ACP. Both performed deductive coding to filter relevant information from their data. Ongoing discussions allowed for the fine-tuning of themes.

Results

End-of-life care planning for people with dementia can encompass a paradox of control. While it may foster a sense of being in control, it may also feel like relinquishing control over future situations. It raises concerns regarding responsibility, as present, former and future wishes may not align. Family caregivers are often responsible for concrete end-of-life decision-making, prompting them to weigh previous wishes against present situations. Further, people with dementia may wish to focus on the present and distance themselves from the future.

Discussion

Advance decisions about end-of-life care are often decontextualized and people may find it difficult to oversee their future conditions and preferences. The widely recognized purpose of ACP to exert control over end-of-life care may need revision to match the needs of people with dementia while they are coping with their condition and an uncertain future.
Sensor data-based probabilistic monitoring of time-history deflections of railway bridges induced by high-speed trains
Authors: Lee Jaebeom , Jeong Seunghoo , Lee Junhwa , Sim Sung-Han , Lee Kyoung-Chan , Lee Young-Joo
Published: January 2022

Structural condition monitoring of railway bridges has been emphasized for guaranteeing the passenger comfort and safety. Various attempts have been made to monitor structural conditions, but many of them have focused on monitoring dynamic characteristics in frequency domain representation which requires additional data transformation. Occurrence of abnormal structural responses, however, can be intuitively detected by directly monitoring the time-history responses, and it may give information including the time to occur the abnormal responses and the magnitude of the dynamic amplification. Therefore, this study suggests a new Bayesian method for directly monitoring the time-history deflections induced by high-speed trains. To train the monitoring model, the data preprocessing of speed estimation and data synchronization are conducted first for the given training data of the raw time-history deflection; the Bayesian inference is then introduced for the derivation of the probability-based dynamic thresholds for each train type. After constructing the model, the detection of the abnormal deflection data is proceeded. The speed estimation and data synchronization are conducted again for the test data, and the anomaly score and ratio are estimated based on the probabilistic monitoring model. A warning is generated if the anomaly ratio is at an unacceptable level; otherwise, the deflection is considered as a normal condition. A high-speed railway bridge in operation is chosen for the verification of the proposed method, in which a probabilistic monitoring model is constructed from displacement time-histories during train passage. It is shown that the model can specify an anomaly of a train-track-bridge system.
Process optimization and removal of phenol formaldehyde resin coating using mechanical erosion process
Authors: Palaniyappan Sabarinathan , Veiravan Annamalai , Kumar Vishal , Mathusoothanaperumal Sukanya Nitin , Veeman Dhinakaran
Published: January 2022

Consumption of coated abrasive discs in various automobile and pipe fitting application is increasing, due to its good surface finish. Coated abrasive disc consists of single layer of abrasive grain bonded to a fibre backing. The major portion of the disc is comprised of fibre backing. But the sustainability of the fibre backing is low and is dumped as waste after usage. The present work deals with the removal of resin coating and recovery of fibre backing from the spent coated abrasive discs using physical separation process such as sand blasting technique. Initially, the recovery experiment was carried out based on L16 orthogonal array. The factors and levels chosen for the experiments were erodent pressure (0.2, 0.4, 0.6 and 0.8 MPa), erodent size (36, 60, 80 and 120 grit), disc orientation (30, 45, 60 and 75°) and number of times flexing (5, 10, 15 and 20). The experimental result shows that erodent size and erodent pressure have a major impact on recovery of the fibre backing. The surface structure of the recovered backing was analysed using scanning electron microscopy and optical microscopy. The recovered backing was very much useful for the coated abrasive industry as the flexible backing and support material for abrasive grain coating.
A Web-based Event History Calendar Approach for Measuring Contraceptive Use Behavior
Authors: West Brady T. , Axinn William G. , Couper Mick P. , Gatny Heather , Schroeder Heather
Published: January 2022

Event history calendars (EHCs) are frequently used in social measurement to capture important information about the time ordering of events in people’s lives and enable inference about the relationships of the events with other outcomes of interest. To date, EHCs have primarily been designed for face-to-face or telephone survey interviewing, and few calendar tools have been developed for more private, self-administered modes of data collection. Web surveys offer benefits in terms of both self-administration, which can reduce social desirability bias, and timeliness. We developed and tested a web application enabling the calendar-based measurement of contraceptive method use histories. These measures provide valuable information for researchers studying family planning and fertility behaviors. This study describes the development of the web application and presents a comparison of data collected from online panels using the application with data from a benchmark face-to-face survey collecting similar measures (the National Survey of Family Growth).
Portal Steal Syndrome From a Large Linton’s Splenorenal Shunt after Liver Transplantation: Successful Endovascular Management Through Off-Label Application of a 30 mm Amplatzer Cardiac Plug
Authors: Centonze Leonardo , Vella Ivan , Morelli Francesco , Checchini Giuliana , De Carlis Riccardo , Rampoldi Antonio , Lauterio Andrea , Andorno Enzo , De Carlis Luciano
Published: January 2022

A 34-year-old patient underwent liver transplantation for progressive hepatic failure in the setting of congenital hepatic fibrosis. In past medical history, the patient had undergone splenectomy with proximal Linton’s splenorenal surgical shunt creation for symptomatic portal hypertension with hypersplenism. The patient developed an early allograft dysfunction, with radiologic evidence of a reduced portal flow associated to portal steal from the patent surgical shunt. The patient was successfully treated through endovascular placement of a 30 mm Amplatzer cardiac plug at the origin of the splenic vein.
Partial Splenic Embolization for the Management of Severe Refractory Thrombocytopenia in Antiphospholipid Syndrome: A Case Report and Literature Review
Authors: Jalili Javad , Pourghorban Ramin , Mahmoudpour Masoud , Akhavi Milani Ali
Published: January 2022

Antiphospholipid syndrome is an autoimmune condition characterized by arteriovenous thromboembolic events. Thrombocytopenia is a common finding among these patients and is typically of mild severity not requiring any treatment. However, severe cases of thrombocytopenia should be treated. Steroids, intravenous immune globulin (IVIG), and immunomodulatory agents are the first-line treatment options, and surgical splenectomy is usually reserved for more severe and refractory cases of thrombocytopenia. Herein, we report the case of a 30-year-old man with primary antiphospholipid syndrome and severe thrombocytopenia. The patient’s thrombocytopenia had been refractory to almost all the medical managements, and surgical splenectomy could not be an option due to the patient’s high-risk condition for surgery. The patient was successfully managed by partial splenic embolization (PSE) which was a unique application of this technique.
How we implemented Continuous Interprofessional Education at a newly established public hospital in Singapore
Authors: Lee Deanna W-C , Dong Chao-Yan , Aw Derrick Chen-Wee
Published: January 2022

Introduction

To promote interprofessional collaboration (IPC), our newly established hospital implemented the Continuing Interprofessional Education (CIPE) initiatives, which included a half-day workshop and 15 sessions of Grand Rounds, with the content focusing on establishing interprofessional patient-centered care pathways, policies, and ultimately to build a community of IPC.

Methods

To evaluate the impact of the CIPE initiatives, 120 staff who attended at least 50% of the CIPE sessions were invited to complete the Interprofessional Attitudes Scale (IPAS).

Results

67.5% of the invited participants completed the survey. The majority of the participants answered “agree” or “strongly agree” for the domains of Teamwork/Roles/Responsibilities, Patient-centeredness, Diversity and Ethics, and Community Centeredness after going through the CIPE initiatives. The Interprofessional Bias domain revealed mixed responses.

Discussions and Implications of practice

The significant contributing factors towards the success of the CIPE Grand Rounds included: (1) the topics were proposed by our staff and centered on clinical practice; (2) the delivery format was interactive, guided by adult learning principles. The mixed responses regarding the presence of biases among the participants suggested that interprofessional biases are deep-rooted in the healthcare setting, and attendance of these CIPE Grand Rounds made participants more acutely aware of these biases. However, more actions are needed to eradicate these biases.
Religious Socialization and Self-Definition Among Black Undergraduate Women Attending Predominantly White Institutions
Authors: Leath Seanna , Hope Meredith O. , Palmer Gordon J. M. , Rose Theda
Published: January 2022

To date, few scholars have explored religious and spiritual socialization among emerging adult Black women. In this study, we analyzed semi-structured interview data from 50 Black undergraduate women to explore associations between childhood religious socialization messages and current religious beliefs in emerging adulthood. Consensual qualitative methods revealed two broad domains and six themes. The first domain, “religious alignment,” included: (1) internalizing religion and (2) educating others on religious beliefs. The second domain, “religious departure,” included: (3) modifying religious expectations to fit developing beliefs, (4) employing religion as a pathway to self-acceptance, (5) picking and choosing battles within their religious community, and (6) choosing an alternate religious or faith system. Findings highlighted how the women started to take ownership of their religious experiences, as well as how they used religious practices, such as prayer, to cope with gendered racism. Authors discuss the implications of emerging adulthood on Black women’s religious identities.
Negative Affectivity and Disinhibition as Moderators of an Interpersonal Pathway to Suicidal Behavior in Borderline Personality Disorder
Authors: Allen Timothy A. , Hallquist Michael N. , Wright Aidan G. C. , Dombrovski Alexandre Y.
Published: January 2022

In this longitudinal study, we examined whether personality traits moderate the link between interpersonal dysfunction and suicidal behavior in a high-risk sample of 458 individuals diagnosed with borderline personality disorder. Participants were assessed annually for up to 30 years (mean number of follow-ups = 7.82). Using multilevel structural equation modeling, we examined (a) longitudinal, within-persons relationships among interpersonal dysfunction, suicidal ideation, and suicide attempts and (b) moderation of these relationships by negative affectivity and disinhibition. Negative affectivity predicted a stronger within-persons coupling between interpersonal dysfunction and suicidal ideation. Disinhibition predicted a stronger coupling between ideation and suicide attempts. Assessing negative affectivity and disinhibition in a treatment setting may guide clinician vigilance toward people at highest risk for interpersonally triggered suicidal behaviors.
The ambiguous impacts of alternative livelihoods on fisher wellbeing in a closed access fish sanctuary in Port Antonio, Jamaica
Authors: Epstein Graham , Alexander Steven M , Marschke Melissa , Campbell Donovan , Armitage Derek
Published: January 2022

Alternative livelihood programs are a central feature of contemporary conservation planning that aim to aid efforts to reduce pressure on natural resources and avoid, minimize, or mitigate the impacts of conservation on the wellbeing of local stakeholders. Evidence of the effectiveness of these programs is, however, decidedly mixed. This research examines the relationship between livelihoods, conservation, and wellbeing among nearshore fishers in Port Antonio, Jamaica, in the context of a recently established marine no-take area. The East Portland Fish Sanctuary was established in 2016 with the aim of supporting the recovery of depleted nearshore fish stocks, marine habitats, and mitigating impacts on local communities and fishers through a range of activities. Mitigation of impacts included efforts to shift pressure from overexploited nearshore fish stocks to offshore resources and to provide support to fishers for training and opportunities to earn income in the tourism sector. The results suggest that additional sources of income tended to enhance the wellbeing of fishers across several dimensions but that it also contributed to additional pressure on nearshore resources. Furthermore, neither tourism nor offshore fishing appear to be particularly promising in terms of their ability to deliver “win-win” outcomes for conservation and wellbeing. Instead, the results suggest that the impacts of different types of alternative livelihoods on conservation and wellbeing are highly variable and perhaps that efforts should be redirected to supporting efforts to provide suitable and acceptable alternatives to spearfishing which appears to have one of the largest overall impacts on individual harvest rates.
Research on seismic performance of traditional Chinese hall-style timber buildings in the Song and Yuan dynasties (960–1368 AD): a case study of the main hall of Baoguo Temple
Authors: Lin Yijie , Chun Qing , Zhang Chengwen , Han Yidan , Fu Hui
Published: January 2022

The hall-style timber frame built in the Song and Yuan dynasties (960–1368 AD) is one of the most important structural prototypes of the traditional timber architecture in East Asia. The current research, through a typical case of the main hall of Baoguo Temple in Ningbo, China, aims to present an accurate and effective seismic performance evaluation method applicable to hall-style timber structures without time–cost expenditure. To obtain more realistic seismic response of hall-style timber frame, a simplified numerical model of the main hall of Baoguo Temple is established based on in situ measurements and low-cycle reversed loading tests results of mortise–tenon joints, moreover, nonlinear static pushover analysis has been performed to quantify the seismic performance levels under five loading conditions. The generalized force–deformation relationship of the timber plastic hinges is modified regarding to the moment–rotation curves of four special mortise–tenon joints. The seismic behaviour of global hall-style timber frame is evaluated through capacity spectrum method and verified by time history analysis, local failure mechanisms are evaluated by the occurrence sequence of plastic hinges. Finally, a performance-based assessment method adequate for the traditional hall-style timber architectures has been proposed with comparison to the current codes. The results have shown that the structural stiffness of the width-direction is less than that of the depth direction due to the asymmetrical configuration of the timber frame, and the building can maintain a stable state under large lateral displacement before collapsing. The inter-storey drift angles of the building under peak ground accelerations of 0.1 g, 0.2 g, and 0.3 g are less than the suggested ultimate values in the current local codes, however, the main hall represents to be more vulnerable to damage when suffer seismic action along the width-direction. This research can provide a reference for seismic performance evaluation and preventive conservation of ancient hall-style timber architectural heritage.
IL-17 crosses the blood–brain barrier to trigger neuroinflammation: a novel mechanism in nitroglycerin-induced chronic migraine
Authors: Chen Hao , Tang Xueqian , Li Jin , Hu Bangyan , Yang Wenqin , Zhan Meng , Ma Tengyun , Xu Shijun
Published: January 2022

Background

Chronic migraine places a disabling burden on patients, which is extensively modeled by the nitroglycerin (NTG)-treated animal model. Although the NF-κB pathway is involved in an increase in CGRP levels and activation of the trigeminal system in the NTG model, the relationship between NTG and neuroinflammation remains unclear. This study aimed to optimize a chronic NTG rat model with hyperalgesia and the ethological capacity for estimating migraine therapies and to further explore the underlying mechanism of NTG-induced migraine.

Methods

Rats were administered different doses of NTG s.c. daily or every 2 d; 30 min and 2 h later, the mechanical threshold was tested. After 9 d, the rats were injected with EB or Cy5.5 for the permeability assay. The other animals were sacrificed, and then, brainstem and caudal trigeminal ganglion were removed to test CGRP, c-Fos and NOS activity; Cytokines levels in the tissue and serum were measured by ELISA; and NF-κB pathway and blood–brain barrier (BBB)-related indicators were analyzed using western blotting. Immunohistochemistry was performed to observe microglial polarization and IL-17A+ T cell migration in the medulla oblongata.

Results

NTG (10 mg/kg, s.c., every 2 d for a total of 5 injections) was the optimal condition, resulting in progressive hyperalgesia and migraine behavior. TNC neuroinflammation with increases in cytokines, CGRP and c-Fos and activation of the NF-κB pathway was observed, and these changes were alleviated by ibuprofen. Furthermore, NTG administration increased BBB permeability by altering the levels functional proteins (RAGE, LRP1, AQP4 and MFSD2A) and structural proteins (ZO-1, Occludin and VE-cadherin-2) to increase peripheral IL-17A permeation into the medulla oblongata, activating microglia and neuroinflammation, and eventually causing hyperalgesia and migraine attack.

Conclusions

This study confirmed that NTG (10 mg/kg, s.c., every 2 d for a total of 5 injections) was the optimal condition to provoke migraine, resulting in mechanical hyperalgesia and observable migraine-like behavior. Furthermore, IL-17A crossed the blood–brain barrier into the medulla oblongata, triggering TNC activation through microglia-mediated neuroinflammation. This process was a novel mechanism in NTG-induced chronic migraine, suggesting that IL-17A might be a novel target in the treatment of migraine.
Mitochondrial potassium channels: A novel calcitriol target
Authors: Olszewska Anna M. , Sieradzan Adam K. , Bednarczyk Piotr , Szewczyk Adam , Żmijewski Michał A.
Published: January 2022

Background

Calcitriol (an active metabolite of vitamin D) modulates the expression of hundreds of human genes by activation of the vitamin D nuclear receptor (VDR). However, VDR-mediated transcriptional modulation does not fully explain various phenotypic effects of calcitriol. Recently a fast non-genomic response to vitamin D has been described, and it seems that mitochondria are one of the targets of calcitriol. These non-classical calcitriol targets open up a new area of research with potential clinical applications. The goal of our study was to ascertain whether calcitriol can modulate mitochondrial function through regulation of the potassium channels present in the inner mitochondrial membrane.

Methods

The effects of calcitriol on the potassium ion current were measured using the patch-clamp method modified for the inner mitochondrial membrane. Molecular docking experiments were conducted in the Autodock4 program. Additionally, changes in gene expression were investigated by qPCR, and transcription factor binding sites were analyzed in the CiiiDER program.

Results

For the first time, our results indicate that calcitriol directly affects the activity of the mitochondrial large-conductance Ca2+-regulated potassium channel (mitoBKCa) from the human astrocytoma (U-87 MG) cell line but not the mitochondrial calcium-independent two-pore domain potassium channel (mitoTASK-3) from human keratinocytes (HaCaT). The open probability of the mitoBKCa channel in high calcium conditions decreased after calcitriol treatment and the opposite effect was observed in low calcium conditions. Moreover, using the AutoDock4 program we predicted the binding poses of calcitriol to the calcium-bound BKCa channel and identified amino acids interacting with the calcitriol molecule. Additionally, we found that calcitriol influences the expression of genes encoding potassium channels. Such a dual, genomic and non-genomic action explains the pleiotropic activity of calcitriol.

Conclusions

Calcitriol can regulate the mitochondrial large-conductance calcium-regulated potassium channel. Our data open a new chapter in the study of non-genomic responses to vitamin D with potential implications for mitochondrial bioenergetics and cytoprotective mechanisms.
Comprehensive analysis of the long noncoding RNA-associated competitive endogenous RNA network in the osteogenic differentiation of periodontal ligament stem cells
Authors: Lai Lingzhi , Wang Zhaodan , Ge Yihong , Qiu Wei , Wu Buling , Fang Fuchun , Xu Huiyong , Chen Zhao
Published: January 2022

Backgroud

The mechanism implicated in the osteogenesis of human periodontal ligament stem cells (PDLSCs) has been investigated for years. Previous genomics data analyses showed that long noncoding RNA (lncRNA), microRNA (miRNA) and messenger RNA (mRNA) have significant expression differences between induced and control human PDLSCs. Competing for endogenous RNAs (ceRNA), as a widely studied mechanism in regenerative medicine, while rarely reported in periodontal regeneration. The key lncRNAs and their ceRNA network might provide new insights into molecular therapies of periodontal regeneration based on PDLSCs.

Results

Two networks reflecting the relationships among differentially expressed RNAs were constructed. One ceRNA network was composed of 6 upregulated lncRNAs, 280 upregulated mRNAs, and 18 downregulated miRNAs. The other network contained 33 downregulated lncRNAs, 73 downregulated mRNAs, and 5 upregulated miRNAs. Functional analysis revealed that 38 GO terms and 8 pathways related with osteogenesis were enriched. Twenty-four osteogenesis-related gene-centred lncRNA-associated ceRNA networks were successfully constructed. Among these pathways, we highlighted MAPK and TGF-beta pathways that are closely related to osteogenesis. Subsequently, subnetworks potentially linking the GO:0001649 (osteoblast differentiation), MAPK and TGF-beta pathways were constructed. The qRT-PCR validation results were consistent with the microarray analysis.

Conclusion

We construct a comprehensively identified lncRNA-associated ceRNA network might be involved in the osteogenesis of PDLSCs, which could provide insights into the regulatory mechanisms and treatment targets of periodontal regeneration.
Diversity and structure of the rhizosphere microbial communities of wild and cultivated ginseng
Authors: Fang Xiaoxue , Wang Huaying , Zhao Ling , Wang Manqi , Sun Mingzhou
Published: January 2022

Background

The resources of wild ginseng have been reducing sharply, and it is mainly dependent on artificial cultivation in China, Korea and Japan. Based on cultivation modes, cultivated ginseng include understory wild ginseng (the seeds or seedlings of cultivated ginseng were planted under the theropencedrymion without human intervention) and farmland cultivated ginseng (grown in farmland with human intervention). Cultivated ginseng, can only be planted on the same plot of land consecutively for several years owing to soilborne diseases, which is mainly because of the variation in the soil microbial community. In contrast, wild ginseng can grow for hundreds of years. However, the knowledge of rhizosphere microbe communities of the wild ginseng is limited.

Result

In the present study, the microbial communities in rhizosphere soils of the three types of ginseng were analyzed by high-throughput sequencing of 16 S rRNA for bacteria and internal transcribed spacer (ITS) region for fungi. In total, 4,381 bacterial operational taxonomic units (OTUs) and 2,679 fungal OTUs were identified in rhizosphere soils of the three types of ginseng. Among them, the shared bacterial OTUs was more than fungal OTUs by the three types of ginseng, revealing fungal communities were to be more affected than bacterial communities. In addition, the composition of rhizosphere microbial communities and bacterial diversity were similar between understory wild ginseng and wild ginseng. However, higher bacterial diversity and lower fungal diversity were found in rhizosphere soils of wild ginseng compared with farmland cultivated ginseng. Furthermore, the relative abundance of Chloroflexi, Fusarium and Alternaria were higher in farmland cultivated ginseng compared to wild ginseng and understory wild ginseng.

Conclusions

Our results showed that composition and diversity of rhizosphere microbial communities were significantly different in three types of ginseng. This study extended the knowledge pedigree of the microbial diversity populating rhizospheres, and provided insights into resolving the limiting bottleneck on the sustainable development of P. ginseng crops, and even the other crops of Panax.
Mapping of dwarfing QTL of Ari1327, a semi-dwarf mutant of upland cotton
Authors: Ma Chenhui , Rehman Abdul , Li Hong Ge , Zhao Zi Bo , Sun Gaofei , Du Xiong Ming
Published: January 2022

Background

Upland Cotton (Gossypium hirsutum L.) has few cotton varieties suitable for mechanical harvesting. The plant height of the cultivar is one of the key features that need to modify. Hence, this study was planned to locate the QTL for plant height in a 60Co γ treated upland cotton semi-dwarf mutant Ari1327.

Results

Interestingly, bulk segregant analysis (BSA) and genotyping by sequencing (GBS) methods exhibited that candidate QTL was co-located in the region of 5.80–9.66 Mb at D01 chromosome in two F2 populations. Using three InDel markers to genotype a population of 1241 individuals confirmed that the offspring’s phenotype is consistent with the genotype. Comparative analysis of RNA-seq between the mutant and wild variety exhibited that Gh_D01G0592 was identified as the source of dwarfness from 200 genes. In addition, it was also revealed that the appropriate use of partial separation markers in QTL mapping can escalate linkage information.

Conclusions

Overwhelmingly, the results will provide the basis to reveal the function of candidate genes and the utilization of excellent dwarf genetic resources in the future.
Salicylic acid mediated immune response of Citrus sinensis to varying frequencies of herbivory and pathogen inoculation
Authors: Ibanez Freddy , Suh Joon Hyuk , Wang Yu , Rivera Monique , Setamou Mamoudou , Stelinski Lukasz L.
Published: January 2022

Background

Plant immunity against pathogens and pests is comprised of complex mechanisms orchestrated by signaling pathways regulated by plant hormones [Salicylic acid (SA) and Jasmonic acid (JA)]. Investigations of plant immune response to phytopathogens and phloem-feeders have revealed that SA plays a critical role in reprogramming of the activity and/or localization of transcriptional regulators via post-translational modifications. We explored the contributing effects of herbivory by a phytopathogen vector [Asian citrus psyllid, Diaphorina citri] and pathogen [Candidatus Liberibacter asiaticus (CaLas)] infection on response of sweet orange [Citrus sinensis (L.) Osbeck] using manipulative treatments designed to mimic the types of infestations/infections that citrus growers experience when cultivating citrus in the face of Huanglongbing (HLB) disease.

Results

A one-time (7 days) inoculation access period with CaLas-infected vectors caused SA-associated upregulation of PR-1, stimulating defense response after a long period of infection without herbivory (270 and 360 days). In contrast, while repeated (monthly) ‘pulses’ of 7 day feeding injury by psyllids stimulated immunity in CaLas-infected citrus by increasing SA in leaves initially (up to 120 days), long-term (270 and 360 days) repeated herbivory caused SA to decrease coincident with upregulation of genes associated with SA metabolism (BMST and DMR6). Similarly, transcriptional responses and metabolite (SA and its analytes) accumulation in citrus leaves exposed to a continuously reproducing population of D. citri exhibited a transitory upregulation of genes associated with SA signaling at 120 days and a posterior downregulation after long-term psyllid (adults and nymphs) feeding (270 and 360 days).

Conclusions

Herbivory played an important role in regulation of SA accumulation in mature leaves of C. sinensis, whether or not those trees were coincidentally infected with CaLas. Our results indicate that prevention of feeding injury inflicted by D. citri from the tritrophic interaction may allow citrus plants to better cope with the consequences of CaLas infection, highlighting the importance of vector suppression as a component of managing this cosmopolitan disease.
The evolution of mean arterial pressure in critically ill patients on vasopressors before and during a trial comparing a specific mean arterial pressure target to usual care
Authors: Masse Marie-Hélène , Adhikari Neill K. J. , Théroux Xavier , Battista Marie-Claude , D’Aragon Frédérick , Pinto Ruxandra , Cohen Alan , Mayette Michaël , St-Arnaud Charles , Kho Michelle , Chassé Michaël , Lebrasseur Martine , Watpool Irene , Porteous Rebecca , Wilcox M. Elizabeth , Lamontagne François
Published: January 2022

Background

In randomized clinical controlled trials, the choice of usual care as the comparator may be associated with better clinician uptake of the study protocol and lead to more generalizable results. However, if care processes evolve to resemble the intervention during the course of a trial, differences between the intervention group and usual care control group may narrow. We evaluated the effect on mean arterial pressure of an unblinded trial comparing a lower mean arterial pressure target to reduce vasopressor exposure, vs. a clinician-selected mean arterial pressure target, in critically ill patients at least 65 years old.

Methods

For this multicenter observational study using data collected both prospectively and retrospectively, patients were recruited from five of the seven trial sites. We compared the mean arterial pressure of patients receiving vasopressors, who met or would have met trial eligibility criteria, from two periods: [1] at least 1 month before the trial started, and [2] during the trial period and randomized to usual care, or not enrolled in the trial.

Results

We included 200 patients treated before and 229 after trial initiation. There were no differences in age (mean 74.5 vs. 75.2 years; p = 0.28), baseline Acute Physiology and Chronic Health Evaluation II score (median 26 vs. 26; p = 0.47) or history of chronic hypertension (n = 126 [63.0%] vs. n = 153 [66.8%]; p = 0.41). Mean of the mean arterial pressure was similar between the two periods (72.5 vs. 72.4 mmHg; p = 0.76).

Conclusions

The initiation of a trial of a prescribed lower mean arterial pressure target, compared to a usual clinician-selected target, was not associated with a change in mean arterial pressure, reflecting stability in the net effect of usual clinician practices over time. Comparing prior and concurrent control groups may alleviate concerns regarding drift in usual practices over the course of a trial or permit quantification of any change.
A large undifferentiated sarcoma of the liver in a 13-year-old girl treated with anatomical resection: a case report and review of the literature
Authors: Bahador Ali , Forooghi Mehdi , Shahriarirad Reza , Geramizadeh Bita , Ataollahi Maryam , Kamran Hooman
Published: January 2022

Background

Undifferentiated embryonal sarcoma of the liver (UESL) is a rare liver tumor accounting for 6–13% of primary liver tumors. Accurate preoperative diagnosis is difficult, with a rather high misdiagnosis rate. Herein, we reported a very large UESL treated with anatomical resection. Our case is amongst the largest pediatric UESLs in the literature.

Case presentation

Herein, we report a 13-year-old girl presenting with right upper quadrant abdominal pain, postprandial vomiting, and abdominal distention, in which radiographic imaging demonstrated a huge UESL (28 × 20 × 12 cm). The patient was treated with partial hepatectomy and the 5 kg tumor was removed. The patient was discharged in good condition, with no significant complaints in her follow-up.

Conclusions

Although different treatment strategies have been reported for UESL cases, anatomical resection is still the main treatment approach, especially for large tumors.
Differing determinants of disability trends among men and women aged 50 years and older
Authors: Chen Ya-Mei , Chiang Tung-Liang , Chen Duan-Rung , Tu Yu-Kang , Yu Hsiao-Wei , Chiu Wan-Yu
Published: January 2022

Background

Researchers have emphasized the importance of examining how different factors affect men’s and women’s functional status over time. To date, the literature is unclear about whether sex affects the rate of change in disability in middle to older age. Researchers have further emphasized the importance of examining how different factors affect men’s and women’s functional status over time. We examined (a) sex differences in disability trends and (b) the determinants of the rate of change in disability for men and women 50 years and older.

Methods

This study utilized the Taiwan Longitudinal Study on Aging Survey, a nationally representative database (four waves of survey data 1996–2007, N = 3429). We modeled and compared the differences in disability trends and the influences of determinants on trends among men and women using multiple-indicator and multiple-group latent growth curves modeling (LGCM). Equality constraints were imposed on 10 determinants across groups.

Results

Once disability began, women progressed toward greater disability 18% faster than men. Greater age added about 1.2 times the burden to the rate of change in disability for women than men (p < 0.001). More comorbidities also added significantly more burden to baseline disability and rate of change in disability among women than men (p < 0.001), but women benefited more from higher education levels in lower baseline disability and slower rate of change. Having a better social network was associated with lower baseline disability among women only (p < 0.05). For both men and women, physically active leisure-time activities were beneficial in lower baseline disability (pmen and women < 0.001) and rate of change in disability (pmen < 0.01; pwomen < 0.05), with no significant differences between groups.

Conclusions

Age may widen the sex gap in the rate of change in disability. However, both sexes benefit from participating in leisure-time activities. Promoting health literacy improves health outcomes and physical function among women.
Potential value of home square-stepping exercises for inactive older adults: an exploratory case study
Authors: Lees Marcus A. , Edwards Jonathon , McCain Jamie E. , Bouchard Danielle R.
Published: January 2022

Background

Most older adults do not engage in regular physical activity. However, more research on options to partake in regular exercise in this population by reducing barriers and enhancing enablers while still reaching benefits is needed.

Methods

Using embedded mixed methods, 10 inactive older adults over the age of 65 completed a 3-week square-stepping exercise intervention to help overcome the initial barriers and activate initial enablers to perform regular exercise. Physical activity level was tracked at home with a pedometer using median steps/day over seven days for pre-post measure. Aerobic intensity while doing square-stepping exercises was quantified via a heart rate monitor in a supervised session. Each participant had an interview asking about barriers and enablers to regular exercise and if the intervention could modify any. Based on initial physical activity a framework matrix was used to pull potential barriers to compare, contrast, and search for patterns between participants with lower and higher initial physical activity levels.

Results

The 3-week square-stepping exercise intervention helped participants overcome barriers such as being uncomfortable in a fitness facility and body image and activate enablers such as the use of home equipment and convenience. The median total steps/day increased by 12% (p = 0.02), and a moderate-intensity level was reached by 80% of the sample participants when performing the square stepping exercise during a supervised session. Common barriers such as having a suitable program, hard to keep the intensity were reported by participants regardless of the initial physical activity level.

Conclusion

Regardless of initial physical activity level, inactive older adults can increase physical activity level at the recommended intensity and overcome common barriers to exercise when performing square-stepping exercises, especially for those intimidated by a fitness facility setting and those concerned with their body image. A longer intervention including more participants using the square-stepping exercises is required to understand if square-stepping exercises can increase the proportion of older adults exercising regularly.
Comparison of different obesity indices associated with type 2 diabetes mellitus among different sex and age groups in Nantong, China: a cross-section study
Authors: Ge Qiwei , Li Min , Xu Zhengcheng , Qi Zhigang , Zheng Huiyan , Cao Yuxin , Huang Hao , Duan Xiaoyang , Zhuang Xun
Published: January 2022

Background

Obesity is associated with type 2 diabetes mellitus (T2DM). However, the obesity index that is most closely related to type 2 diabetes remains controversial. Therefore, the aim of this study was to compare the associations of five anthropometric indices (body mass index [BMI], body adiposity index, waist circumference [WC], waist-to-hip ratio, and waist-to-height ratio [WHtR]) with T2DM among Chinese adults divided into four groups according to sex and age.

Methods

A total of 4007 adult participants (1669 men and 2338 women) were included in the study. Odds ratios (ORs) and 95% confidence intervals were used with binary logistic regression models to estimate the risk of T2DM for each obesity index. Furthermore, we compared the area under the receiver operating characteristic curve (AUC) of each obesity index for the criterion of T2DM under the influence of risk factors.

Results

WC had the highest OR (3.211 and 1.452) and AUC (0.783 and 0.614) in both age groups of men. However, WHtR (OR = 2.366, AUC = 0.771) and BMI (OR = 1.596, AUC = 0.647) were the optimal criteria for predicting T2DM among females in the 18–59 and ≥ 60 years age groups, respectively.

Conclusions

This study suggests that there is a positive association between obesity-related anthropometric indices and T2DM in different sex and age groups. WC appears to be the optimal anthropometric index for predicting T2DM in men. The optimal obesity indices related to T2DM were WHtR and BMI for women aged 18–59 and ≥ 60 years, respectively.
Assessing the scalability of an integrated falls prevention service for community-dwelling older people: a mixed methods study
Authors: Calnan Susan , Lee Karen , McHugh Sheena
Published: January 2022

Background

There is growing acknowledgement of the need for a phased approach to scaling up health interventions, beginning with an assessment of ‘scalability’, that is, the capacity of an individual intervention to be scaled up. This study aims to assess the scalability of a multi-component integrated falls prevention service for community-dwelling older people and to examine the applicability of the Intervention Scalability Assessment Tool (ISAT). The ISAT consists of 10 domains for consideration when determining the scalability of an intervention, and each domain comprises a series of questions aimed at examining readiness for scale-up.

Methods

Multiple methods were used sequentially as recommended by the ISAT: a review of policy documents, results from a service evaluation and falls-related literature; one-to-one interviews (n = 11) with key stakeholders involved in management and oversight of the service; and a follow-up online questionnaire (n = 10) with stakeholders to rate scalability and provide further feedback on reasons for their scores.

Results

Three of the ISAT domains were rated highly by the participants. Analysis of the qualitative feedback and documents indicated that the issue of falls prevention among older people was of sufficient priority to warrant scale-up of the service and that the service aligned with national health policy priorities. Some participants also noted that benefits of the service could potentially outweigh costs through reduced hospital admissions and serious injuries such as hip fracture. The remaining domains received a moderate score from participants, however, indicating considerable barriers to scale-up. In the qualitative feedback, barriers identified included the perceived need for more healthcare staff to deliver components of the service, for additional infrastructure such as adequate room space, and for an integrated electronic patient management system linking primary and secondary care and to prevent duplication of services.

Conclusions

Plans to scale up the service are currently under review given the practical barriers that need to be addressed. The ISAT provides a systematic and structured framework for examining the scalability of this multi-component falls prevention intervention, although the iterative nature of the process and detailed and technical nature of its questions require considerable time and knowledge of the service to complete.
Management of hypertension and renin-angiotensin-aldosterone system blockade in adults with diabetic kidney disease: Association of British Clinical Diabetologists and the Renal Association UK guideline update 2021
Authors: Banerjee D. , Winocour P. , Chowdhury T. A. , De P. , Wahba M. , Montero R. , Fogarty D. , Frankel A. H. , Karalliedde J. , Mark P. B. , Patel D. C. , Pokrajac A. , Sharif A. , Zac-Varghese S. , Bain S. , Dasgupta I. ,   On behalf of the Association of British Clinical Diabetologists and The Renal Association
Published: January 2022

People with type 1 and type 2 diabetes are at risk of developing progressive chronic kidney disease (CKD) and end-stage kidney failure. Hypertension is a major, reversible risk factor in people with diabetes for development of albuminuria, impaired kidney function, end-stage kidney disease and cardiovascular disease. Blood pressure control has been shown to be beneficial in people with diabetes in slowing progression of kidney disease and reducing cardiovascular events. However, randomised controlled trial evidence differs in type 1 and type 2 diabetes and different stages of CKD in terms of target blood pressure. Activation of the renin-angiotensin-aldosterone system (RAAS) is an important mechanism for the development and progression of CKD and cardiovascular disease. Randomised trials demonstrate that RAAS blockade is effective in preventing/ slowing progression of CKD and reducing cardiovascular events in people with type 1 and type 2 diabetes, albeit differently according to the stage of CKD. Emerging therapy with sodium glucose cotransporter-2 (SGLT-2) inhibitors, non-steroidal selective mineralocorticoid antagonists and endothelin-A receptor antagonists have been shown in randomised trials to lower blood pressure and further reduce the risk of progression of CKD and cardiovascular disease in people with type 2 diabetes. This guideline reviews the current evidence and makes recommendations about blood pressure control and the use of RAAS-blocking agents in different stages of CKD in people with both type 1 and type 2 diabetes.