bluuebunny/crossref_metadata_2025 huggingface dataset:
Neural Decision Tree: A New Tool for Building Forecast Models for Plasmasphere Dynamics
Authors: Lu Yu L. , Zhelavskaya Irina S. , Wang Chunming
Published: June 2022

The Neural Decision Tree (NDT) is a hybrid supervised machine‐learning algorithm that combines the self‐limiting property of a decision tree (Classification and Regression Tree [CART]) algorithm with the artificial neural network. We demonstrate the use of NDT for a regression problem of building a prediction model for the plasmasphere electron density with solar and geomagnetic measurements as inputs. Our work replicates the work by Zhelavskaya et al. reported in their 2017 article (I. S. Zhelavskaya et al., 2017, https://doi.org/10.1002/2017JA024406) to show that NDT makes available sophisticated network layout for building a predictive model, thus taking advantage of deep‐learning potential of the neural network. We also demonstrate that with the ability to automatically select an appropriate network layout, as well as, effective initialization, the NDT algorithm allows research scientists in space weather to focus more of their attention on physically and statistically relevant aspects of using machine‐learning techniques. In fact, our example highlights the fact that the basic assumptions of standard supervise machine‐learning problems are often unsatisfied in real‐world space weather applications. Greater attention to these fundamental issues may create significantly different solutions to space weather forecast problems.
Timing and Order of Extreme Drought and Wetness Determine Bioclimatic Sensitivity of Tree Growth
Authors: Wu Xiuchen , Liu Hongyan , Hartmann Henrik , Ciais Philippe , Kimball John S. , Schwalm Christopher R. , Camarero Jesús Julio , Chen Anping , Gentine Pierre , Yang Yuting , Zhang Shulei , Li Xiaoyan , Xu Chongyang , Zhang Wen , Li Zongshan , Chen Deliang
Published: June 2022

Tree resistance to extreme droughts and post‐drought recovery are sensitive to the drought timing. However, how the bioclimatic sensitivity of tree growth may vary with the timing and order of extreme droughts and wetness is still poorly understood. Here, we quantified the bioclimatic sensitivity of tree growth in the period of 1951–2013 under different seasonal extreme drought/wetness regimes over the extra‐tropical Northern Hemisphere, using 1,032 tree ring chronologies from 121 gymnosperm and angiosperm species. We found a negative asymmetry in tree growth under regimes with seasonal extreme droughts. With extreme drought, tree growth in arid and temperate dry regions is more negatively impacted by pre‐growing‐season (PGS) extreme droughts. Clade‐wise, angiosperms are more sensitive to PGS water availability, and gymnosperms to legacy effects of the preceding tree growth conditions in temperate dry and humid regions. Our finding of divergent bioclimatic legacy effects underscores contrasting trends in forest responses to drought across different ecoregions and functional groups in a more extreme climate.
Domain Adaptation in Automatic Picking of Phase Velocity Dispersions Based on Deep Learning
Authors: Song Weibin , Feng Xuping , Zhang Gongheng , Gao Lina , Yan Binpeng , Chen Xiaofei
Published: June 2022

Ambient seismic noise tomography has been applied to probe the Earth's structure. To accurately map geological structures, a considerable amount of time is required to pick fundamental and higher modes of dispersion curves. We used frequency‐Bessel (F‐J) transform to calculate phase velocity‐frequency diagrams to obtain more higher modes of dispersion curves from the cross‐correlation function. Several studies have recently focused on picking dispersions automatically using deep learning to reduce time consumption. However, the generalization of neural networks has a degradation for untrained diagrams to some degree. Here, based on domain adaptation in computer vision, we used gamma transform to change the image contrast of the phase velocity‐frequency diagrams, rendering the test data closer to the training data. Introducing domain adaptation into the dispersion region extraction effectively improves the generalization of the neural network. Here, the dispersion regions are the regions in the phase velocity‐frequency diagram located around the dispersion curves where the energy is above a given threshold. We validated our method by using phase velocity‐frequency diagrams from different areas. We used one synthetic phase velocity‐frequency diagram and three phase velocity‐frequency diagrams of different areas to test domain adaptation. In particular, we tested one phase velocity‐frequency diagram that belongs to the same area for the training diagram, except for the processing steps. The results showed that our domain adaptation method successfully enhanced the generalization of the neural network. After domain adaptation, our trained network could effectively extract more higher modes of dispersion regions than before. Our dispersion curves picking method combined with domain adaptation can pick sufficient dispersion information for numerous phase velocity‐frequency diagrams. Furthermore, our method can facilitate the study of ambient noise tomography and illumination of the Earth's interiors. Our research provides a strategy for enhancing the generalization of neural networks for other deep learning‐based geophysical image segmentation tasks.
Quantifying the Role of Atmospheric and Surface Albedo on Polar Amplification Using Satellite Observations and CMIP6 Model Output
Authors: Södergren A. H. , McDonald A. J.
Published: June 2022

Understanding polar amplification (PA) and its underlying processes is key to accurately predicting the climate system's response to increasing anthropogenic forcings. We examine the amplified warming in the Arctic and Antarctic in 17 global climate models from the Coupled Model Intercomparison Project 6 (CMIP6) against satellite data. Large hemispheric differences in PA strength was found in the CMIP6 models. Changes in surface temperature and strength of PA is closely coupled to changes in albedo. The planetary albedo of Earth (αp) is partitioned into a component associated with surface albedo (defined as surface contribution to planetary albedo, ), and a component associated with atmospheric albedo (atmospheric contribution to planetary albedo, ). To assess the hemispheric differences in PA strengths, the relative importance of and were investigated. The surface reflection looks different as seen at the surface (defined as surface albedo, αsurf) compared to (as seen at the top of the atmosphere). We find a stronger correlation between surface temperature and αsurf in the Arctic than in the Antarctic, with correlation coefficients of −0.94 and −0.88, respectively. Interestingly, the correlation for surface temperature and is stronger in the Antarctic than in the Arctic with correlation coefficients of −0.93 and −0.90, respectively. In the southern high latitudes, albedo changes at the surface are more important than changes in the atmosphere, while the opposite applies in the northern high latitudes. Surface temperature changes in the low‐ and mid‐latitudes are strongly associated with changes in , dominated by changes in cloud properties.
Orographic‐Induced Strong Wind Associated With a Low‐Pressure System Under Clear‐Air Condition During ICE‐POP 2018
Authors: Tsai Chia‐Lun , Kim Kwonil , Liou Yu‐Chieng , Kim Jung‐Hoon , Lee YongHee , Lee GyuWon
Published: June 2022

A strong wind event under clear‐air conditions during the 2018 Winter Olympic and Paralympic games in Pyeongchang, Korea, was examined using various datasets. High spatiotemporal resolution wind information was obtained by Doppler lidars, automatic weather stations, wind profiler, sounding observations, reanalysis datasets under the International Collaborative Experiments for Pyeongchang 2018 Olympic and Paralympic winter games (ICE‐POP 2018). This study aimed to understand the possible mechanisms of localized strong winds across a high mountainous area and on the leeside associated with the underlying large‐scale pattern of a low‐pressure system (LPS). The evolution of surface winds shows quite different patterns, exhibiting intensification of strong winds in the leeside and periodically persistent strong winds in upstream mountainous areas with the approaching LPS. The surface wind speed was intensified from ∼3 to ∼12 m s−1 (gusts were stronger than 20 m s−1 above the ground) at a surface station in the leeside. A budget analysis of the horizontal momentum equation suggested that the pressure gradient force (PGF) contributed from adiabatic warming and the passage of LPS was the main factor in the acceleration of the surface wind in the leeward side of the mountains. The detailed 3D winds revealed that the PGF also modulated the background winds at the mountainous station, which caused persistent strong and periodic winds (range of ∼7 to ∼12 m s−1) related to the channeling effect. The evidence showed that under the same synoptic condition of a LPS, different mechanisms are important for strong winds in determining the strength and persistence of orographic‐induced strong winds under clear‐air conditions.
Enhanced blue-light excited cyan-emitting persistent luminescence of BaLu2Al2Ga2SiO12:Ce3+, Bi3+ phosphors for AC-LEDs via defect modulation
Authors: Yuan Weihong , Pang Ran , Wang Shangwei , Tan Tao , Li Chengyu , Wang Chaowei , Zhang Hongjie
Published: June 2022

Alternating current light-emitting diodes (AC-LEDs) have received significant attention from both academia and industry due to their remarkable benefits of more compact volume, cheaper manufacturing cost, greater energy usage efficiency, and longer service life. One of the most significant challenges for AC-LEDs is the flicker effect, which is mainly caused by the unavoidable 5–20 ms dimming time. Aiming to reduce the flicker effect, we designed a series of excellent blue-light excited cyan-emitting persistent luminescence (PersL) phosphors BaLu2Al2Ga2SiO12:Ce3+, Bi3+ via defect engineering of co-doping Bi3+. Interestingly, we found that co-doping Bi3+ not only effectively enhanced the PersL intensity, but also regulated the PersL lifetime of this phosphors. As the Bi3+ co-doping concentration increases to 0.01, the τ80 value (the time when the PersL intensity decreases to 80% of the initial intensity) increases from 0.24 to 19.61 ms, which proves to be effective in compensating the flicker effect of AC-LEDs. A new method of generating white light emission during the dimming time through adding the blue-light excited cyan PersL phosphor to the original orange-red PersL phosphor was proposed and an AC-LED lamp with a decreased percent flicker of 48.15% was fabricated, which is significantly better than the other currently reported AC-LED devices based on PersL phosphors. These results demonstrate that BaLu2Al2Ga2SiO12:Ce3+, Bi3+ might be an attractive material for low-flicker AC-LEDs.
Chronic spinal cord injury repair by NT3-chitosan only occurs after clearance of the lesion scar
Authors: Zhao Can , Rao Jia-Sheng , Duan Hongmei , Hao Peng , Shang Junkui , Fan Yubo , Zhao Wen , Gao Yudan , Yang Zhaoyang , Sun Yi Eve , Li Xiaoguang
Published: June 2022

Spinal cord injury (SCI) is a severe damage usually leading to limb dysesthesia, motor dysfunction, and other physiological disability. We have previously shown that NT3-chitosan could trigger an acute SCI repairment in rats and non-human primates. Due to the negative effect of inhibitory molecules in glial scar on axonal regeneration, however, the role of NT3-chitosan in the treatment of chronic SCI remains unclear. Compared with the fresh wound of acute SCI, how to handle the lesion core and glial scars is a major issue related to chronic-SCI repair. Here we report, in a chronic complete SCI rat model, establishment of magnetic resonance-diffusion tensor imaging (MR-DTI) methods to monitor spatial and temporal changes of the lesion area, which matched well with anatomical analyses. Clearance of the lesion core via suction of cystic tissues and trimming of solid scar tissues before introducing NT3-chitosan using either a rigid tubular scaffold or a soft gel form led to robust neural regeneration, which interconnected the severed ascending and descending axons and accompanied with electrophysiological and motor functional recovery. In contrast, cystic tissue extraction without scar trimming followed by NT3-chitosan injection, resulted in little, if any regeneration. Taken together, after lesion core clearance, NT3-chitosan can be used to enable chronic-SCI repair and MR-DTI-based mapping of lesion area and monitoring of ongoing regeneration can potentially be implemented in clinical studies for subacute/chronic-SCI repair.
Ellagic acid improves benign prostate hyperplasia by regulating androgen signaling and STAT3
Authors: Park Woo Yong , Song Gahee , Park Ja Yeon , Ahn Kwang Seok , Kwak Hyun Jeong , Park Jinbong , Lee Jun Hee , Um Jae-Young
Published: June 2022

Abstracts

Benign prostate hyperplasia (BPH) is an age-related disease in men characterized by the growth of prostate cells and hyperproliferation of prostate tissue. This condition is closely related to chronic inflammation. In this study, we highlight the therapeutic efficacy of ellagic acid (EA) for BPH by focusing on the AR signaling axis and STAT3. To investigate the effect of EA on BPH, we used EA, a phytochemical abundant in fruits and vegetables, to treat testosterone propionate (TP)-induced BPH rats and RWPE-1 human prostate epithelial cells. The EA treatment reduced prostate weight, prostate epithelial thickness, and serum DHT levels in the TP-induced BPH rat model. In addition, EA improved testicular injury by increasing antioxidant enzymes in testis of the BPH rats. EA reduced the protein levels of AR, 5AR2, and PSA. It also induced apoptosis by regulating Bax, Bcl_xL, cytochrome c, caspase 9, and caspase 3 with increasing mitochondrial dynamics. Furthermore, EA reduced the expression of IL-6, TNF-α, and NF-κB, as well as phosphorylation of STAT3 and IκBα. These findings were also confirmed in TP-treated RWPE-1 cells. Overall, our data provide evidence of the role of EA in improving BPH through inhibition of AR and the STAT3 pathway.
Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction
Authors: Liu Zhenhua , Yang Nannan , Dong Jie , Tian Wotu , Chang Lisa , Ma Jinghong , Guo Jifeng , Tan Jieqiong , Dong Ao , He Kaikai , Zhou Jingheng , Cinar Resat , Wu Junbing , Salinas Armando G. , Sun Lixin , Kumar Mantosh , Sullivan Breanna T. , Oldham Braden B. , Pitz Vanessa , Makarious Mary B. , Ding Jinhui , Kung Justin , Xie Chengsong , Hawes Sarah L. , Wang Lupeng , Wang Tao , Chan Piu , Zhang Zhuohua , Le Weidong , Chen Shengdi , Lovinger David M. , Blauwendraat Cornelis , Singleton Andrew B. , Cui Guohong , Li Yulong , Cai Huaibin , Tang Beisha
Published: June 2022

Endocannabinoid (eCB),2-arachidonoyl-glycerol (2-AG), the most abundant eCB in the brain, regulates diverse neural functions. Here we linked multiple homozygous loss-of-function mutations in 2-AG synthase diacylglycerol lipase β (DAGLB) to an early onset autosomal recessive Parkinsonism. DAGLB is the main 2-AG synthase in human and mousesubstantia nigra(SN) dopaminergic neurons (DANs). In mice, the SN 2-AG levels were markedly correlated with motor performance during locomotor skill acquisition. Genetic knockdown ofDaglbin nigral DANs substantially reduced SN 2-AG levels and impaired locomotor skill learning, particularly the across-session learning. Conversely, pharmacological inhibition of 2-AG degradation increased nigral 2-AG levels, DAN activity and dopamine release and rescued the locomotor skill learning deficits. Together, we demonstrate thatDAGLB-deficiency contributes to the pathogenesis of Parkinsonism, reveal the importance of DAGLB-mediated 2-AG biosynthesis in nigral DANs in regulating neuronal activity and dopamine release, and suggest potential benefits of 2-AG augmentation in alleviating Parkinsonism.
Single-cell characterization of step-wise acquisition of carboplatin resistance in ovarian cancer
Authors: Wenzel Alexander T. , Champa Devora , Venkatesh Hrishi , Sun Si , Tsai Cheng-Yu , Mesirov Jill P. , Bui Jack D. , Howell Stephen B. , Harismendy Olivier
Published: June 2022

The molecular underpinnings of acquired resistance to carboplatin are poorly understood and often inconsistent between in vitro modeling studies. After sequential treatment cycles, multiple isogenic clones reached similar levels of resistance, but significant transcriptional heterogeneity. Gene-expression based virtual synchronization of 26,772 single cells from 2 treatment steps and 4 resistant clones was used to evaluate the activity of Hallmark gene sets in proliferative (P) and quiescent (Q) phases. Two behaviors were associated with resistance: (1) broad repression in the P phase observed in all clones in early resistant steps and (2) prevalent induction in Q phase observed in the late treatment step of one clone. Furthermore, the induction of IFNα response in P phase or Wnt-signaling in Q phase were observed in distinct resistant clones. These observations suggest a model of resistance hysteresis, where functional alterations of the P and Q phase states affect the dynamics of the successive transitions between drug exposure and recovery, and prompts for a precise monitoring of single-cell states to develop more effective schedules for, or combination of, chemotherapy treatments.
Predicting the risk of pipe failure using gradient boosted decision trees and weighted risk analysis
Authors: Barton Neal Andrew , Hallett Stephen Henry , Jude Simon Richard , Tran Trung Hieu
Published: June 2022

Pipe failure prediction models are essential for informing proactive management decisions. This study aims to establish a reliable prediction model returning the probability of pipe failure using a gradient boosted tree model, and a specific segmentation and grouping of pipes on a 1 km grid that associates localised characteristics. The model is applied to an extensive UK network with approximately 40,000 km of pipeline and a 14-year failure history. The model was evaluated using the Receiver Operator Curve and Area Under the Curve (0.89), briers score (0.007) and Mathews Correlation Coefficient (0.27) for accuracy, indicating acceptable predictions. A weighted risk analysis is used to identify the consequence of a pipe failure and provide a graphical representation of high-risk pipes for decision makers. The weighted risk analysis provided an important step to understanding the consequences of the predicted failure. The model can be used directly in strategic planning, which sets long-term key decisions regarding maintenance and potential replacement of pipes.
Pupillometry and electroencephalography in the digit span task
Authors: Pavlov Yuri G. , Kasanov Dauren , Kosachenko Alexandra I. , Kotyusov Alexander I. , Busch Niko A.
Published: June 2022

This dataset consists of raw 64-channel EEG, cardiovascular (electrocardiography and photoplethysmography), and pupillometry data from 86 human participants recorded during 4 minutes of eyes-closed resting and during performance of a classic working memory task – digit span task with serial recall. The participants either memorized or just listened to sequences of 5, 9, or 13 digits presented auditorily every 2 seconds. The dataset can be used for (1) developing algorithms for cognitive load discrimination and detection of cognitive overload; (2) studying neural (event-related potentials and brain oscillations) and peripheral (electrocardiography, photoplethysmography, and pupillometry) physiological signals during encoding and maintenance of each sequentially presented memory item; (3) correlating cognitive load and individual differences in working memory to neural and peripheral physiology, and studying the relationship between the physiological signals; (4) integration of the physiological findings with the vast knowledge coming from behavioral studies of verbal working memory in simple span paradigms. The data are shared in Brain Imaging Data Structure (BIDS) format and freely available on OpenNeuro (https://openneuro.org/datasets/ds003838).
A focus groups study on data sharing and research data management
Authors: Donaldson Devan Ray , Koepke Joshua Wolfgang
Published: June 2022

Data sharing can accelerate scientific discovery while increasing return on investment beyond the researcher or group that produced them. Data repositories enable data sharing and preservation over the long term, but little is known about scientists’ perceptions of them and their perspectives on data management and sharing practices. Using focus groups with scientists from five disciplines (atmospheric and earth science, computer science, chemistry, ecology, and neuroscience), we asked questions about data management to lead into a discussion of what features they think are necessary to include in data repository systems and services to help them implement the data sharing and preservation parts of their data management plans. Participants identified metadata quality control and training as problem areas in data management. Additionally, participants discussed several desired repository features, including: metadata control, data traceability, security, stable infrastructure, and data use restrictions. We present their desired repository features as a rubric for the research community to encourage repository utilization. Future directions for research are discussed.
A curated, ontology-based, large-scale knowledge graph of artificial intelligence tasks and benchmarks
Authors: Blagec Kathrin , Barbosa-Silva Adriano , Ott Simon , Samwald Matthias
Published: June 2022

Research in artificial intelligence (AI) is addressing a growing number of tasks through a rapidly growing number of models and methodologies. This makes it difficult to keep track of where novel AI methods are successfully – or still unsuccessfully – applied, how progress is measured, how different advances might synergize with each other, and how future research should be prioritized. To help address these issues, we created the Intelligence Task Ontology and Knowledge Graph (ITO), a comprehensive, richly structured and manually curated resource on artificial intelligence tasks, benchmark results and performance metrics. The current version of ITO contains 685,560 edges, 1,100 classes representing AI processes and 1,995 properties representing performance metrics. The primary goal of ITO is to enable analyses of the global landscape of AI tasks and capabilities. ITO is based on technologies that allow for easy integration and enrichment with external data, automated inference and continuous, collaborative expert curation of underlying ontological models. We make the ITO dataset and a collection of Jupyter notebooks utilizing ITO openly available.
Charge balance calculations for mixed salt systems applied to a large dataset from the built environment
Authors: Godts Sebastiaan , Steiger Michael , Orr Scott Allan , De Kock Tim , Desarnaud Julie , De Clercq Hilde , Cnudde Veerle
Published: June 2022

Understanding salt mixtures in the built environment is crucial to evaluate damage phenomena. This contribution presents charge balance calculations applied to a dataset of 11412 samples taken from 338 sites, building materials showing signs of salt deterioration. Each sample includes ion concentrations of Na+, K+, Mg2+, Ca2+, Cl−, NO3−, and SO42−adjusted to reach charge balance for data evaluation. The calculation procedure follows two distinct pathways: i) an equal adjustment of all ions, ii) adjustments to the cations in sequence related to the solubility of the theoretical solids. The procedure applied to the dataset illustrates the quantification of salt mixture compositions and highlights the extent of adjustments applied in relation to the sample mass to aid interpretation. The data analysis allows the identification of theoretical carbonates that could influence the mixture behavior. Applying the charge balance calculations to the dataset validated common ions found in the built environment and the identification of three typical mixture compositions. Additionally, the data can be used as direct input for thermodynamic modeling.
Transcriptome dataset of omental and subcutaneous adipose tissues from gestational diabetes patients
Authors: Salcedo-Tacuma David , Bonilla Leonardo , Montes Maria Cristina Geney , Gonzalez Jorge Ernesto Niño , Gutierrez Sandra Milena Sanchez , Chirivi Miguel , Contreras G. Andres
Published: June 2022

Gestational diabetes (GD) is one of the most prevalent metabolic diseases in pregnant women worldwide. GD is a risk factor for adverse pregnancy outcomes, including macrosomia and preeclampsia. Given the multifactorial etiology and the complexity of its pathogenesis, GD requires advanced omics analyses to expand our understanding of the disease. Next generation RNA sequencing (RNA-seq) was used to evaluate the transcriptomic profile of subcutaneous and omental adipose tissues (AT) collected from patients with gestational diabetes and matched controls. Samples were harvested during cesarean delivery. Results show differences based on anatomical location and provide whole-transcriptome data for further exploration of gene expression patterns unique to GD patients.
Lightsheet optical tweezer (LOT) for optical manipulation of microscopic particles and live cells
Authors: Mondal Partha Pratim , Baro Neptune , Singh Ankur , Joshi Prakash , Basumatary Jigmi
Published: June 2022

Optical trapping and patterning cells or microscopic particles is fascinating. We developed a light sheet-based optical tweezer to trap dielectric particles and live HeLa cells. The technique requires the generation of a tightly focussed diffraction-limited light-sheet realized by a combination of cylindrical lens and high NA objective lens. The resultant field is a focussed line (along x-axis) perpendicular to the beam propagation direction (z-axis). This is unlike traditional optical tweezers that are fundamentally point-traps and can trap one particle at a time. Several spherical beads undergoing Brownian motion in the solution are trapped by the lightsheet gradient potential, and the time (to reach trap-centre) is estimated from the video captured at 230 frames/s. High-speed imaging of beads with increasing laser power shows a steady increase in trap stiffness with a maximum of 0.00118 pN/nm at 52.5 mW. This is order less than the traditional point-traps, and hence may be suitable for applications requiring delicate optical forces. On the brighter side, light sheet tweezer (LOT) can simultaneously trap multiple objects with the distinct ability to manipulate them in the transverse (xy) plane via translation and rotation. However, the trapped beads displayed free movement along the light-sheet axis (x-axis), exhibiting a single degree of freedom. Furthermore, the tweezer is used to trap and pattern live HeLa cells in various shapes and structures. Subsequently, the cells were cultured for a prolonged period of time (> 18 h), and cell viability was ascertained. We anticipate that LOT can be used to study constrained dynamics of microscopic particles and help understand the patterned cell growth that has implications in optical imaging, microscopy, and cell biology.
Dual-expression system for blue fluorescent protein optimization
Authors: Papadaki Stavrini , Wang Xinyue , Wang Yangdong , Zhang Hanbin , Jia Su , Liu Shuhong , Yang Minghan , Zhang Dongdong , Jia Jie-Min , Köster Reinhard W. , Namikawa Kazuhiko , Piatkevich Kiryl D.
Published: June 2022

Spectrally diverse fluorescent proteins (FPs) provide straightforward means for multiplexed imaging of biological systems. Among FPs fitting standard color channels, blue FPs (BFPs) are characterized by lower brightness compared to other spectral counterparts. Furthermore, available BFPs were not systematically characterized for imaging in cultured mammalian cells and common model organisms. Here we introduce a pair of new BFPs, named Electra1 and Electra2, developed through hierarchical screening in bacterial and mammalian cells using a novel dual-expression vector. We performed systematic benchmarking of Electras against state-of-art BFPs in cultured mammalian cells and demonstrated their utility as fluorescent tags for structural proteins. The Electras variants were validated for multicolor neuroimaging in Caenorhabditis elegans, zebrafish larvae, and mice in comparison with one of the best in the class BFP mTagBFP2 using one-photon and two-photon microscopy. The developed BFPs are suitable for multicolor imaging of cultured cells and model organisms in vivo. We believe that the described dual-expression vector has a great potential to be adopted by protein engineers for directed molecular evolution of FPs.
Neonatal neurologic emergencies requiring access to paediatric emergency units: a retrospective observational study
Authors: Falsaperla Raffaele , Vitaliti Giovanna , Sciacca Monica , Tardino Lucia , Marino Simona Domenica , Marino Silvia , Moscheo Carla , Meli Mariaclaudia , Vitaliti Maria Concetta , Barbagallo Massimo , Di Stefano Vita Antonella , Saporito Marco Andrea Nicola , Ruggieri Martino
Published: June 2022

Herein, authors present a retrospective, multi-center study to determine the number of accesses to Pediatric Emergency Unit (PEU) of patients within 28 days of life, admitted to (1) the Acute and Emergency Pediatric Unit, San Marco University Hospital, Catania, Italy; (2) Garibaldi Hospital for Emergency Care, Catania, Italy; (3) Cannizzaro Hospital for Emergency Care, Catania, Italy. We included neonates admitted for neurologic problems, from January 2015 to December 2020, to the 1—Acute and Emergency Access of the San Marco University Hospital, Catania, Italy [observation center 1 (OC1)]; 2—Garibaldi Hospital for Emergency Care, Catania, Italy (Observation Center 2—OC2); 3—Cannizzaro Hospital for Emergency Care, Catania, Italy (Observation Center 3—OC3). For each patient, we evaluated the severity of urgency, by studying the admission triage-coloured codes, the clinical data at admission and the discharge diagnosis. Neonates who had access to PEU were 812 in the OC1, 3720 in the OC2, and 748 in the OC3 respectively; 69 (8.4%), 138 (3.7%), and 55 (7.4%) was the proportion of neonatal accesses for neurological conditions. We observed that in the study period, the three hospitals had an important decrease of pediatric accesses to their PEU, but the proportion of neonates who had access to the OC1 for neurologic diseases, with respect to the total neonatal accesses, remained stable. We found that the most frequent neurologic disease for which newborns had access to PEU was Cyanosis, (46.1% of all neonatal accesses). Apnea was the second most frequent cause, with a number of 76 accesses (29%). In the literature there are numerous studies on the assessment of diseases that most frequently concern the pediatric patient in an emergency room, but there are very few references on neonatal accesses for urgent neurologic diseases. Therefore, appropriate training is required to avoid unnecessary tests without overlooking potentially serious conditions.
Comparison between frequency-doubling technology perimetry and standard automated perimetry in early glaucoma
Authors: Kim Seong Ah , Park Chan Kee , Park Hae-Young Lopilly
Published: June 2022

This study aimed to find out the significance of the difference between frequency-doubling technology perimetry (FDT) and standard automated perimetry (SAP) in terms of the detected visual field (VF) damage, and evaluate associated factors to SAP–FDT difference in early glaucoma. Glaucoma patients in early stage (MD better than − 6.0 decibel, 96 eyes) were included in this cross-sectional study. We subtracted mean deviation (MD) and pattern standard deviation (PSD) of FDT from those of SAP, respectively. Additionally, we counted significantly depressed points of P < 5% and P < 1% on the pattern deviation probability plot of both FDT and SAP and defined eyes with significant SAP–FDT difference when the number of abnormal points were greater than 4 points on FDT. We measured lamina cribrosa depth (LCD) and lamina cribrosa curvature index (LCCI) for structural parameters of the optic nerve head from images using enhanced depth imaging of the optical coherence tomography (OCT). Peripapillary vessel density (VD) and presence of microvasculature dropout (MvD), the complete loss of choriocapillaris in localized regions of parapapillary atrophy, was evaluated using deep layer map of OCT angiography (OCT-A) for vascular parameters. Peripheral nasal step (PNS) group had an isolated glaucomatous VF defect within nasal periphery outside 10° of fixation. Parafoveal scotoma (PFS) group had an isolated glaucomatous VF defect within 12 points of a central 10˚ radius. Eyes with significant SAP–FDT difference showed higher detection of MvD on deep layer map of OCT-A, greater LCD, and greater LCCI (all P < 0.05, respectively). In logistic regression analysis, frequent presence of MvD, less presence of disc hemorrhage, and greater LCD were significantly associated with significant SAP–FDT difference. Sub-analysis was performed in eyes with PNS (50 eyes) and PFS (46 eyes). SAP–FDT difference of MD value showed positive association with peripapillary VD on deep layer of OCT-A, which was significant in eyes with PFS compared to eyes with PNS. SAP–FDT difference of PSD value showed negative association with LCCI and LCD, which was significant in eyes with PNS compared to eyes with PFS. Glaucomatous eyes classified by the difference of the detected VF damage on FDT versus SAP showed different clinical features. Greater SAP–FDT difference was significantly associated with structural parameters such as LCD and LCCI. Less SAP–FDT difference was associated with presence of disc hemorrhage and lower deep layer peripepillary VD. There is possibility to use the difference of SAP and FDT to identify associated risk factors in glaucoma patients.
In-vitro antiproliferative efficacy of Abrus precatorius seed extracts on cervical carcinoma
Authors: Kaur Amritpal , Sharma Yash , Kumar Anoop , Ghosh Madhumita P. , Bala Kumud
Published: June 2022

Abrus precatorius is a tropical medicinal plant with multiple medicinal benefits whose seeds have not yet been studied against cervical cancer. Herein, we have assessed the antioxidant and antiproliferative properties of seed extracts (ethyl acetate and 70% ethanol) prepared from Soxhlet and Maceration extraction methods against Hep2C and HeLa Cells. We observed that the APE (Sox) extract had a significantly higher total flavonoid content, APA (Mac) extract had a high total phenolic content, and APA (Sox) extract had a high total tannin content. Further, HPLC analysis of extracts revealed the presence of tannic acid and rutin. Moreover, APA (Sox) exhibited the highest free radical scavenging activity. APE (Mac) had the best antiproliferative activity against Hep2C cells, while APA (Sox) had the best antiproliferative activity against HeLa cells. In Hep2C cells, APE (Mac) extract revealed the highest SOD, catalase activity, GSH content, and the lowest MDA content, whereas APA (Mac) extract demonstrated the highest GST activity. In HeLa cells, APA (Sox) extract showed the highest SOD, GST activity, GSH content, and the least MDA content, whereas APA (Mac) extract showed the highest catalase activity. Lastly, docking results suggested maximum binding affinity of tannic acid with HER2 and GCR receptors. This study provides evidence that A. precatorius seed extracts possess promising bioactive compounds with probable anticancer and antioxidant properties against cervical cancer for restricting tumor growth.
Long-distance air pressure differences correlate with European rain
Authors: Müller-Plath Gisela , Lüdecke Horst-Joachim , Lüning Sebastian
Published: June 2022

Precipitation in Europe shows natural fluctuations that differ considerably between seasons and geographical regions. A number of studies have linked local or seasonal rainfall variability to various long-distance air pressure differences in north–south or west–east direction. This paper presents the first continent-wide analysis of European rainfall variability on a month-by-month and country-by-country basis. We calculated Pearson r values for unsmoothed monthly rainfall data of 39 European countries over the period 1950–2019 with five potential climatic drivers, namely the North Atlantic Oscillation (NAO), the Arctic Oscillation (AO), the North Sea Caspian Pattern (NCP), and two indices of Mediterranean Oscillation (MOI2, WeMOI). For a variety of countries and months we found substantial and statistically significant r values of up to r = 0.7 and more. The dynamic temporal-spatial evolution of the Pearson correlations was mapped out across the continent, tracking the gradual or abrupt expansion, displacement and subsequent waning of the various effects over the course of the year. The correlation analysis was complemented by best subset multiple regression, controlling for intercorrelation of the potential drivers. Our results may help to improve short- to midterm rainfall prognoses in Europe and provide important calibration data for the further refinement of climate models.
FAM111A is dispensable for electrolyte homeostasis in mice
Authors: Ilenwabor Barnabas P. , Schigt Heidi , Kompatscher Andreas , Bos Caro , Zuidscherwoude Malou , van der Eerden Bram C. J. , Hoenderop Joost G. J. , de Baaij Jeroen H. F.
Published: June 2022

Autosomal dominant mutations in FAM111A are causative for Kenny-Caffey syndrome type 2. Patients with Kenny-Caffey syndrome suffer from severe growth retardation, skeletal dysplasia, hypoparathyroidism, hypocalcaemia, hyperphosphataemia and hypomagnesaemia. While recent studies have reported FAM111A to function in antiviral response and DNA replication, its role in regulating electrolyte homeostasis remains unknown. In this study, we assessed the role of FAM111A in the regulation of serum electrolyte balance using a Fam111a knockout (Fam111a−/−) C57BL/6 N mouse model. Fam111a−/− mice displayed normal weight and serum parathyroid hormone (PTH) concentration and exhibited unaltered magnesium, calcium and phosphate levels in serum and 24-hour urine. Expression of calciotropic (including Cabp28k, Trpv5, Klotho and Cyp24a1), magnesiotropic (including Trpm6, Trpm7, Cnnm2 and Cnnm4) and phosphotropic (Slc20a1, Slc20a2, Slc34a1 and Slc34a3) genes in the kidneys, duodenum and colon were not affected by Fam111a depletion. Only Slc34a2 expression was significantly upregulated in the duodenum, but not in the colon. Analysis of femurs showed unaffected bone morphology and density in Fam111a−/− mice. Kidney and parathyroid histology were also normal in Fam111a−/− mice. In conclusion, our study is the first to characterise the function of FAM111A in vivo and we report that mice lacking FAM111A exhibit normal electrolyte homeostasis on a standard diet.
Daily mother-infant skin-to-skin contact and maternal mental health and postpartum healing: a randomized controlled trial
Authors: Cooijmans Kelly H. M. , Beijers Roseriet , Brett Bonnie E. , de Weerth Carolina
Published: June 2022

This randomized controlled trial examined the effects of a daily hour of mother-infant skin-to-skin contact (SSC) during the first five postnatal weeks, compared to care-as-usual, on maternal depressive (primary outcome), anxiety, stress, fatigue, pain, and delivery-related post-traumatic stress symptoms (PTSS). Prenatal symptom severity and touch discomfort were examined as moderators. Mothers and full-term infants were randomly allocated to SSC or care-as-usual conditions and followed during the first postnatal year. For the total group (intention-to-treat analyses), care-as-usual mothers showed an increase of anxiety symptoms from week 2 to 12, while SSC mothers displayed a stability of anxiety symptoms. Also, care-as-usual mothers showed an initial decrease in fatigue followed by an increase, while SSC mothers showed a decrease from week 2 to 12. In per-protocol analyses, including only the SSC dyads who adhered to SSC guidelines, findings on anxiety, but not fatigue, were replicated. No SSC effects were found for depressive, stress, and pain symptoms. No moderator, dose–response, or 52-week follow-up effects were found. PTSS were low with little variation; consequently, analyses were discontinued. Daily SSC in healthy mother-infant dyads may reduce anxiety and fatigue symptoms, but not depressive, stress, and pain symptoms, during the early postpartum period. Replication studies are recommended.
Methicillin-resistant Staphylococcus aureus colonization among medicine and health science students, Arba Minch University, Ethiopia
Authors: Mekuriya Ermiyas , Manilal Aseer , Aklilu Addis , Woldemariam Melat , Hailu Tadiwos , Wasihun Biresaw
Published: June 2022

Individuals with Methicillin-Resistant Staphylococcus aureus (MRSA) colonized nasal cavities were at greater risk of developing the infection and can serve as potential reservoirs of transmission. Aim of this study is to determine the extent of nasal carriage and associated factors linked to MRSA in medical and health science students of Arba Minch University (AMU), Ethiopia, who are much prone. An institution based cross-sectional study was conducted at AMU from 01st August through 30th November, 2020 by means of a systematic sampling technique using a structured questionnaire. Nasal swabs samples were collected and S. aureus were identified following standard microbiological methods. Methicillin resistance was tested using cefoxitin disk and antimicrobial susceptibility tests were performed by Kirby-Bauer disk diffusion. Biofilm forming ability was phenotypically detected by micro-titer plate assay. Descriptive statistics and multivariable logistic regression analysis were done by Statistical Package for Social Service (SPSS) version 25. The overall prevalence of Staphylococcus aureus and MRSA were 27.1% (70/258) and 7.4% (19/258) respectively. Methicillin-Resistant S. aureus carriage were higher among medical interns, 16.9% (11/65). Isolates in general were co-resistant to antibiotics, such as trimethoprim-sulfamethoxazole (63.2%) and tetracycline (48.4%). Multidrug resistance (MDR) were observed among 52.6% (10/19) of the isolates. Besides, 31.4% (6/19) of MRSA were biofilm producers and all of them were MDR. Multivariable analysis showed that mean exposure for > 2 years to hospital settings [p = 0.048, AOR: 4.99, 95% CI 1.01–24.66] and the habit of sharing clothing and sports equipment [p = 0.017, AOR: 5.43, 95% CI 1.35–21.83] were statistically significant. The overall prevalence of nasal colonized MRSA among students were comparatively lower than that observed in other studies done in Ethiopia itself. An alarming factor is that, 60% of MDR-MRSA were biofilm producers.
Mid-pregnancy poly(I:C) viral mimic disrupts placental ABC transporter expression and leads to long-term offspring motor and cognitive dysfunction
Authors: Monteiro V. R. S. , Andrade C. B. V. , Gomes H. R. , Reginatto M. W. , Império G. E. , Fontes K. N. , Spiess D. A. , Rangel-Junior W. S. , Nascimento V. M. O. , Lima C. O. S. , Sousa R. P. C. , Bloise F. F. , Matthews S. G. , Bloise E. , Pimentel-Coelho P. M. , Ortiga-Carvalho T. M.
Published: June 2022

Limited information is available about the effect of mid-pregnancy viral infections on the placental expression of efflux transporters and offspring behavior. We hypothesized that maternal exposure to polyinosinic-polycytidylic acid [poly(I:C)], a synthetic double-stranded RNA viral mimic, would impair placental cell turnover, the expression of selected ABC transporters and adult offspring behavior. C57BL/6 mice were administered poly(I:C) (10 mg/Kg;ip) or vehicle at gestational day (GD) 13.5 (mid-pregnancy). Dams were euthanized for blood collection 4 h after injection, fetal and placental collection at GD18.5 or allowed to deliver spontaneously at term. At GD 13.5, poly(I:C) induced an acute pro-inflammatory response characterized by an increase in maternal plasma levels of IL-6, CXCL-1 and CCL-2/MCP-1. At GD 18.5, poly(I:C) decreased cell proliferation/death in the labyrinthine and increased cell death in the junctional zones, characterizing a disruption of placental cell turnover. Abca1 and Abcg1 immunolabelling was decreased in the labyrinthine zone, whereas Abca1, Abcg1 and breast cancer resistance transporter (Bcrp) expression increased in the junctional zone. Moreover, adult offspring showed motor and cognitive impairments in the Rotarod and T-water maze tests. These results indicate that viral infection during mid-pregnancy may disrupt relevant placental efflux transporters, as well as placental cell turnover and offspring behavior in adult life.
Comparative study of oral versus parenteral crocin in mitigating acrolein-induced lung injury in albino rats
Authors: Rashad Walaa Abdelhaliem , Sakr Samar , Domouky Ayat M.
Published: June 2022

Acrolein (Ac) is the second most commonly inhaled toxin, produced in smoke of fires, tobacco smoke, overheated oils, and fried foods; and usually associated with lung toxicity. Crocin (Cr) is a natural carotenoid with a direct antioxidant capacity. Yet, oral administration of crocin as a natural rout is doubtful, because of poor absorbability. Therefore, the current study aimed to compare the potential protective effect of oral versus intraperitoneal (ip) crocin in mitigating Ac-induced lung toxicity. 50 Adult rats were randomly divided into 5 equal groups; Control (oral-saline and ip-saline) group, Cr (oral-Cr and ip-Cr) group, Ac group, oral-Cr/Ac group, and ip-Cr/Ac group; for biochemical, histopathological, and immunohistochemical investigations. Results indicated increased oxidative stress and inflammatory biomarkers in lungs of Ac-treated group. Histopathological and immunohistochemical examinations revealed lung edema, infiltration, fibrosis, and altered expression of apoptotic and anti-apoptotic markers. Compared to oral-Cr/Ac group, the ip-Cr/Ac group demonstrated remarkable improvement in the oxidative, inflammatory, and apoptotic biomarkers, as well as the histopathological alterations. In conclusion, intraperitoneal crocin exerts a more protective effect on acrolein-induced lung toxicity than the orally administered crocin.