bluuebunny/crossref_metadata_2025 huggingface dataset:
Migration and Removal of Labile Cadmium Contaminants in Paddy Soils by Electrokinetic Remediation without Changing Soil pH
Authors: Luan Yajun , Xu Junzeng , Zhou Jing , Wang Haiyu , Han Fengxiang , Wang Kechun , Lv Yuping
Published: March 2022

Electrokinetic remediation (EKR) is a viable, advanced cleaning strategy that can permanently reduce the toxicity of soil contaminants. However, EKR is prone to causing changes in soil pH. The negative impacts must be minimized if field-scale application is to be realized. In this study, EKR with polarity reversal was used to avoid soil pH polarization and to clean up cadmium (Cd)-contaminated paddy soils. Results showed that Cd desorbed from oxidizable and residual fractions to labile and easily available parts. Soil moisture content above 0.35 g g−1 was conductive to achieving the desirable Cd-migration rate. The exchangeable Cd phase eventually migrated from both ends of that soil compartment towards the intermediate. Moreover, the addition of citric acid at the concentration of 0.1 mol L−1 was an effective enhancement strategy. The methodology enriched Cd contaminants to specific sites. The technology can be used for electrokinetic-assisted phytoremediation during the rice growing period. Hyperaccumulator is planted in the intermediate area to remove the Cd contaminants. On the other hand, Cd removal is achieved in the region close to the electrodes. The present study provides a theoretical basis for in situ remediation. It has a wider significance for field-scale application.
Psychological Resilience to Suicidal Experiences in People with Non-Affective Psychosis: A Position Paper
Authors: Gooding Patricia A. , Harris Kamelia , Haddock Gillian
Published: March 2022

It is important to understand the psychological factors which underpin pathways to suicidal experiences. It is equally as important to understand how people develop and maintain resilience to such psychological factors implicated in suicidal experiences. Exploring optimal routes to gaining this understanding of resilience to suicidal thoughts and acts in people with severe mental health problems, specifically non-affective psychosis, was the overarching aim of this position paper. There are five central suggestions: 1. investigating resilience to suicidal experiences has been somewhat over-looked, especially in those with severe mental health problems such as schizophrenia; 2. it appears maximally enlightening to use convergent qualitative, quantitative and mixed research methods to develop a comprehensive understanding of resilience to suicide; 3. relatedly, involving experts-by-experience (consumers) in suicide research in general is vital, and this includes research endeavours with a focus on resilience to suicide; 4. evidence-based models of resilience which hold the most promise appear to be buffering, recovery and maintenance approaches; and 5. there is vast potential for contemporary psychological therapies to develop and scaffold work with clients centred on building and maintaining resilience to suicidal thoughts and acts based on different methodological and analytical approaches which involve both talking and non-talking approaches.
Quantitative Evaluation of Waste Separation Management Policies in the Yangtze River Delta Based on the PMC Index Model
Authors: Liu Fang , Liu Zhi
Published: March 2022

Numerous policies have been formulated and implemented to strengthen waste separation management activities in many countries. Waste separation management policies (WSMPs) must be evaluated as the precondition for reducing deviations from policy implementation and improving waste separation performance. Based on text mining technology and the construction of a policy modeling consistency (PMC) index model, we conducted a quantitative evaluation of 22 WSMPs issued by central governmental departments and provinces in the Yangtze River Delta, China from 2013 to 2021 and analyzed their optimization paths. The results suggest that the PMC index of the selected WSMPs has an upward trend. The average PMC index of 22 WSMPs was 6.906, indicating good quality in the policy texts. The PMC index identified seven, nine, five, and one of the policies as being perfect, excellent, good, and acceptable, respectively. The characteristics of WSMPs were further illustrated through PMC surface charts. Based on this, optimization paths for WSMPs with lower PMC indexes are proposed, which indicate that existing WSMPs have great potential for optimization in terms of harsher constraint regulations, context-appropriate incentives, and cultivation of market participants. Finally, this study provides a beneficial reference for similar cities or countries to improve their performance in the management of waste separation and environmental protection.
What Influences Miners’ Safety Risk Perception?
Authors: Zhang Shu , Hua Xinyu , Huang Ganghai , Shi Xiuzhi , Li Dandan
Published: March 2022

The risks faced by the mining industry have always been prominent for every walk of life in China. As the direct cause of accidents, individual unsafe behaviors are closely related to their risk perception. So, it is important to explore the factors affecting miners’ risk perception and analyze the influencing mechanisms between these factors and risk perception. The questionnaire survey method was used to collect the data of risk perception from nearly 400 respondents working in metal mines in China. Exploratory factor analysis and confirmatory factor analysis were used to analyze and process collected data. The impact of four factors affecting miners’ risk perception was verified, namely: organizational safety atmosphere, organizational trust, knowledge level, and risk communication. Then, regression analysis, Pearson correlation analysis, and structural equation model analysis were used to examine the effect of the four influencing factors on miners’ risk perception. The four influencing factors all have a positive impact on miners’ risk perception; knowledge level has the largest explained variation of miners’ risk perception, followed by risk communication. Organizational trust and organizational safety atmosphere have an indirect and positive impact on miners’ risk perception intermediated by knowledge level and risk communication. The results offer four important aspects of mine safety management to help miners establish quick and accurate risk perception, thereby reducing unsafe behaviors and avoiding accidents.
Three Growth Spurts in Global Physical Activity Policies between 2000 and 2019: A Policy Document Analysis
Authors: Muzenda Trish , Shung-King Maylene , Lambert Estelle Victoria , Brugulat Panés Anna , Weimann Amy , McCreedy Nicole , Tatah Lambed , Mapa-Tassou Clarisse , Govia Ishtar , Were Vincent , Oni Tolu
Published: March 2022

Non-communicable diseases (NCDs) contribute significantly to global mortality and are of particular concern in growing urban populations of low- and-middle income countries (LMICs). Physical inactivity is a key NCD determinant and requires urgent addressing. Laudable global and regional efforts to promote physical activity are being made, but the links between physical activity (PA), NCD reduction, and integrated intersectoral approaches to reducing obesogenic environments are not consistently made. This study applied a document analysis approach to global PA and NCD policies to better understand the current global policy environment and how this may facilitate integrated PA promotion. A total of 34 global policies related to PA, from different sectors, were analyzed. PA policy in mitigation of NCDs has evolved exponentially, with a progression towards addressing structural determinants alongside individual behavior change. The global PA agenda is primarily driven by the World Health Organization. Intersectoral collaboration is importantly regarded, but the contributions of other sectors, outside of health, education, transport, and urban planning, are less clear. Improving PA among key sub-populations—women, girls, and adolescents—requires greater policy consideration. It is imperative for PA-relevant sectors at all levels to recognize the links with NCDs and work towards integrated policy and practice in mitigation of the rising NCD pandemic.
A New Target of Dental Pulp-Derived Stem Cell-Based Therapy on Recipient Bone Marrow Niche in Systemic Lupus Erythematosus
Authors: Sonoda Soichiro , Yamaza Takayoshi
Published: March 2022

Recent advances in mesenchymal stem/stromal cell (MSC) research have led us to consider the feasibility of MSC-based therapy for various diseases. Human dental pulp-derived MSCs (hDPSCs) have been identified in the dental pulp tissue of deciduous and permanent teeth, and they exhibit properties with self-renewal and in vitro multipotency. Interestingly, hDPSCs exhibit superior immunosuppressive functions toward immune cells, especially T lymphocytes, both in vitro and in vivo. Recently, hDPSCs have been shown to have potent immunomodulatory functions in treating systemic lupus erythematosus (SLE) in the SLE MRL/lpr mouse model. However, the mechanisms underlying the immunosuppressive efficacy of hDPSCs remain unknown. This review aims to introduce a new target of hDPSC-based therapy on the recipient niche function in SLE.
Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice
Authors: Dogru Murat , Kojima Takashi , Simsek Cem , Nagata Taeko , Tsubota Kazuo
Published: March 2022

The purpose of this study was to investigate the changes in E-FABP in the salivary and lacrimal glands of the Sjögren syndrome (SS) model non-obese diabetic mice (NOD). Cotton thread and ocular vital staining tests were performed on 10-week NOD male mice (n = 24) and age- and sex-matched wild-type (WT) mice (n = 25). Tear and saliva samples were collected at sacrifice for E-FABP ELISA assays. Salivary and lacrimal gland specimens underwent immunohistochemistry stainings for E-FABP. Real-time RT-PCR was also performed for the quantification of mRNA expression levels in the salivary and lacrimal glands. Corneal vital staining scores in the NOD mice were significantly higher compared with those for the wild-type mice (p = 0.0001). The mean tear E-FABP level showed a significantly lower concentration in the NOD mice (p = 0.001). The mean saliva E-FABP level also showed a significantly lower concentration in the NOD mice (p = 0.04). Immunohistochemistry revealed intense E-FABP staining in the LG acinar epithelium and less intense staining in the acinar epitheliae of the SGs in the NOD mice compared to the WT mice. Real-time RT-PCR for the mRNA expression of E-FABP showed a significantly decreased expression in the SG and a significant increase in the LG of the NOD mice compared to the WT mice. In conclusion, the E-FABP showed marked alterations in the tear film, saliva, lacrimal, and salivary glands of the NOD mouse, which may help explain the ocular surface changes in relation to the dry eye disease in this SS model mouse and keratoconjunctivitis sicca in SS patients.
The Urinary Level of Injury Biomarkers Is Not Univocally Reflective of the Extent of Toxic Renal Tubular Injury in Rats
Authors: Sancho-Martínez Sandra M. , Herrero María , Fontecha-Barriuso Miguel , Mercado-Hernández Joana , López-Hernández Francisco J.
Published: March 2022

Nephrotoxicity is a major cause of intrinsic acute kidney injury (AKI). Because renal tissue damage may occur independently of a reduction in glomerular filtration rate and of elevations in plasma creatinine concentration, so-called injury biomarkers have been proposed to form part of diagnostic criteria as reflective of tubular damage independently of renal function status. We studied whether the urinary level of NGAL, KIM-1, GM2AP, t-gelsolin, and REGIIIb informed on the extent of tubular damage in rat models of nephrotoxicity, regardless of the etiology, moment of observation, and underlying pathophysiology. At a time of overt AKI, urinary biomarkers were measured by Western blot or ELISA, and tubular necrosis was scored from histological specimens stained with hematoxylin and eosin. Correlation and regression studies revealed that only weak relations existed between biomarkers and tubular damage. Due to high interindividual variability in the extent of damage for any given biomarker level, urinary injury biomarkers did not necessarily reflect the extent of the underlying tissue injury in individual rats. We contended, in this work, that further pathophysiological contextualization is necessary to understand the diagnostic significance of injury biomarkers before they can be used for renal tubular damage severity stratification in the context of nephrotoxic and, in general, intrinsic AKI.
Phosphorylation but Not Oligomerization Drives the Accumulation of Tau with Nucleoporin Nup98
Authors: Diez Lisa , Kapinos Larisa E. , Hochmair Janine , Huebschmann Sabrina , Dominguez-Baquero Alvaro , Vogt Amelie , Rankovic Marija , Zweckstetter Markus , Lim Roderick Y. H. , Wegmann Susanne
Published: March 2022

Tau is a neuronal protein that stabilizes axonal microtubules (MTs) in the central nervous system. In Alzheimer’s disease (AD) and other tauopathies, phosphorylated Tau accumulates in intracellular aggregates, a pathological hallmark of these diseases. However, the chronological order of pathological changes in Tau prior to its cytosolic aggregation remains unresolved. These include its phosphorylation and detachment from MTs, mislocalization into the somatodendritic compartment, and oligomerization in the cytosol. Recently, we showed that Tau can interact with phenylalanine-glycine (FG)-rich nucleoporins (Nups), including Nup98, that form a diffusion barrier inside nuclear pore complexes (NPCs), leading to defects in nucleocytoplasmic transport. Here, we used surface plasmon resonance (SPR) and bio-layer interferometry (BLI) to investigate the molecular details of Tau:Nup98 interactions and determined how Tau phosphorylation and oligomerization impact the interactions. Importantly, phosphorylation, but not acetylation, strongly facilitates the accumulation of Tau with Nup98. Oligomerization, however, seems to inhibit Tau:Nup98 interactions, suggesting that Tau-FG Nup interactions occur prior to oligomerization. Overall, these results provide fundamental insights into the molecular mechanisms of Tau-FG Nup interactions within NPCs, which might explain how stress-and disease-associated posttranslational modifications (PTMs) may lead to Tau-induced nucleocytoplasmic transport (NCT) failure. Intervention strategies that could rescue Tau-induced NCT failure in AD and tauopathies will be further discussed.
Myokines and Resistance Training: A Narrative Review
Authors: Zunner Beate E. M. , Wachsmuth Nadine B. , Eckstein Max L. , Scherl Lukas , Schierbauer Janis R. , Haupt Sandra , Stumpf Christian , Reusch Laura , Moser Othmar
Published: March 2022

In the last few years, the muscular system has gained attention due to the discovery of the muscle-secretome and its high potency for retaining or regaining health. These cytokines, described as myokines, released by the working muscle, are involved in anti-inflammatory, metabolic and immunological processes. These are able to influence human health in a positive way and are a target of research in metabolic diseases, cancer, neurological diseases, and other non-communicable diseases. Therefore, different types of exercise training were investigated in the last few years to find associations between exercise, myokines and their effects on human health. Particularly, resistance training turned out to be a powerful stimulus to enhance myokine release. As there are different types of resistance training, different myokines are stimulated, depending on the mode of training. This narrative review gives an overview about resistance training and how it can be utilized to stimulate myokine production in order to gain a certain health effect. Finally, the question of why resistance training is an important key regulator in human health will be discussed.
Novel Insights on Nitric Oxide Synthase and NO Signaling in Ascidian Metamorphosis
Authors: Locascio Annamaria , Vassalli Quirino Attilio , Castellano Immacolata , Palumbo Anna
Published: March 2022

Nitric oxide (NO) is a pivotal signaling molecule involved in a wide range of physiological and pathological processes. We investigated NOS/NO localization patterns during the different stages of larval development in the ascidia Ciona robusta and evidenced a specific and temporally controlled pattern. NOS/NO expression starts in the most anterior sensory structures of the early larva and progressively moves towards the caudal portion as larval development and metamorphosis proceeds. We here highlight the pattern of NOS/NO expression in the central and peripheral nervous system of Ciona larvae which precisely follows the progression of neural signals of the central pattern generator necessary for the control of the movements of the larva towards the substrate. This highly dynamic localization profile perfectly matches with the central role played by NO from the first phase of settlement induction to the next control of swimming behavior, adhesion to substrate and progressive tissue resorption and reorganization of metamorphosis itself.
Modeling PCDH19-CE: From 2D Stem Cell Model to 3D Brain Organoids
Authors: Borghi Rossella , Magliocca Valentina , Trivisano Marina , Specchio Nicola , Tartaglia Marco , Bertini Enrico , Compagnucci Claudia
Published: March 2022

PCDH19 clustering epilepsy (PCDH19-CE) is a genetic disease characterized by a heterogeneous phenotypic spectrum ranging from focal epilepsy with rare seizures and normal cognitive development to severe drug-resistant epilepsy associated with intellectual disability and autism. Unfortunately, little is known about the pathogenic mechanism underlying this disease and an effective treatment is lacking. Studies with zebrafish and murine models have provided insights on the function of PCDH19 during brain development and how its altered function causes the disease, but these models fail to reproduce the human phenotype. Induced pluripotent stem cell (iPSC) technology has provided a complementary experimental approach for investigating the pathogenic mechanisms implicated in PCDH19-CE during neurogenesis and studying the pathology in a more physiological three-dimensional (3D) environment through the development of brain organoids. We report on recent progress in the development of human brain organoids with a particular focus on how this 3D model may shed light on the pathomechanisms implicated in PCDH19-CE.
Systematic Analysis and Expression Profiles of the 4-Coumarate: CoA Ligase (4CL) Gene Family in Pomegranate (Punica granatum L.)
Authors: Wang Yuying , Guo Linhui , Zhao Yujie , Zhao Xueqing , Yuan Zhaohe
Published: March 2022

4-Coumarate:CoA ligase (4CL, EC6.2.1.12), located at the end of the phenylpropanoid metabolic pathway, regulates the metabolic direction of phenylpropanoid derivatives and plays a pivotal role in the biosynthesis of flavonoids, lignin, and other secondary metabolites. In order to understand the molecular characteristics and potential biological functions of the 4CL gene family in the pomegranate, a bioinformatics analysis was carried out on the identified 4CLs. In this study, 12 Pg4CLs were identified in the pomegranate genome, which contained two conserved amino acid domains: AMP-binding domain Box I (SSGTTGLPKGV) and Box II (GEICIRG). During the identification, it was found that Pg4CL2 was missing Box II. The gene cloning and sequencing verified that this partial amino acid deletion was caused by genome sequencing and splicing errors, and the gene cloning results corrected the Pg4CL2 sequence information in the ‘Taishanhong’ genome. According to the phylogenetic tree, Pg4CLs were divided into three subfamilies, and each subfamily had 1, 1, and 10 members, respectively. Analysis of cis-acting elements found that all the upstream sequences of Pg4CLs contained at least one phytohormone response element. An RNA-seq and protein interaction network analysis suggested that Pg4CL5 was highly expressed in different tissues and may participate in lignin synthesis of pomegranate. The expression of Pg4CL in developing pomegranate fruits was analyzed by quantitative real-time PCR (qRT-PCR), and the expression level of Pg4CL2 demonstrated a decreasing trend, similar to the trend of flavonoid content, indicating Pg4CL2 may involve in flavonoid synthesis and pigment accumulation. Pg4CL3, Pg4CL7, Pg4CL8, and Pg4CL10 were almost not expressed or lowly expressed, the expression level of Pg4CL4 was higher in the later stage of fruit development, suggesting that Pg4CL4 played a crucial role in fruit ripening. The expression levels of 4CL genes were significantly different in various fruit development stages. The results laid the foundation for an in-depth analysis of pomegranate 4CL gene functions.
Mesenchymal Stromal Cells from Healthy and Inflamed Human Gingiva Respond Differently to Porphyromonas gingivalis
Authors: Bekić Marina , Radanović Marina , Đokić Jelena , Tomić Sergej , Eraković Mile , Radojević Dušan , Duka Miloš , Marković Dejan , Marković Milan , Ismaili Bashkim , Bokonjić Dejan , Čolić Miodrag
Published: March 2022

Gingiva-Derived Mesenchymal Stromal Cells (GMSCs) have been shown to play an important role in periodontitis. However, how P. gingivalis, one of the key etiological agents of the disease, affects healthy (H)- and periodontitis (P)-GMSCs is unknown. To address this problem, we established 10 H-GMSC and 12 P-GMSC lines. No significant differences in morphology, differentiation into chondroblasts and adipocytes, expression of characteristic MSCS markers, including pericyte antigens NG2 and PDGFR, were observed between H- and P-GMSC lines. However, proliferation, cell size and osteogenic potential were higher in P-GMSCs, in contrast to their lower ability to suppress mononuclear cell proliferation. P. gingivalis up-regulated the mRNA expression of IL-6, IL-8, MCP-1, GRO-α, RANTES, TLR-2, HIF-1α, OPG, MMP-3, SDF-1, HGF and IP-10 in P-GMSCs, whereas only IL-6, MCP-1 and GRO-α were up-regulated in H-GMSCs. The expression of MCP-1, RANTES, IP-10 and HGF was significantly higher in P-GMSCs compared to H-GMSCs, but IDO1 was lower. No significant changes in the expression of TLR-3, TLR-4, TGF-β, LAP, IGFBP4 and TIMP-1 were observed in both types of GMSCs. In conclusion, our results suggest that P-GMSCs retain their pro-inflammatory properties in culture, exhibit lower immunosuppressive potential than their healthy counterparts, and impaired regeneration-associated gene induction in culture. All these functions are potentiated significantly by P. gingivalis treatment.
The COVID-19 Cell Signalling Problem: Spike, RAGE, PKC, p38, NFκB & IL-6 Hyper-Expression and the Human Ezrin Peptide, VIP, PKA-CREB Solution
Authors: Holms Rupert
Published: March 2022

SARS-CoV-2 infection inhibits interferon expression, while hyper-activating innate-immune signalling and expression of pro-inflammatory cytokines. SARS-CoV-2 proteins: Spike, M and nsp6, nsp12 and nsp13 inhibit IFR3-mediated Type-1-interferon defence, but hyper-activate intracellular signalling, which leads to dysfunctional expression of pro-inflammatory cytokines, particularly IL-1β IL-6, IL-8, and TNFα. Ezrin, a sub-membrane adaptor-protein, organises multi-protein-complexes such as ezrin+NHERF1+NHE+CFTR, which control the density and location of ACE2 receptor expression on the luminal surface of airway-epithelial-cells, as well as determining susceptibility to SARS-CoV-2 infection. This protein complex is vital for lung-surfactant production for efficient gas-exchange. Ezrin also forms multi-protein-complexes that regulate signalling kinases; Ras, PKC, PI3K, and PKA. m-RAGE is a pattern-recognition-receptor of the innate immune system that is triggered by AGEs, which are chemically modified proteins common in the elderly and obese. m-RAGE forms multi-protein complexes with ezrin and TIRAP, a toll-like-receptor adaptor-protein. The main cause of COVID-19 is not viral infection but pro-inflammatory p38MAPK signalling mediated by TLRs and RAGE. In contrast, it appears that activated ezrin+PKA signalling results in the activation of transcription-factor CREB, which suppresses NFκB mediated pro-inflammatory cytokine expression. In addition, competition between ezrin and TIRAP to form multi-protein-complexes on membrane PIP2-lipid-rafts is a macromolecular-switch that changes the priority from innate immune activation programs to adaptive immune activation programs. Human Vasoactive Intestinal Peptide (VIP), and Human Ezrin Peptides (HEP-1 and RepG3) probably inhibit COVID-19 by activating the ezrin+PKA and ras>Raf>MEK>ERK>RSK>CREB>IL-10 signalling, which favours activation of adaptive immunity programs and inhibition of the dysfunctional innate-inflammation, the cause of COVID-19. HEP-1, RepG3, and VIP in individual human volunteers and in small clinical studies have been shown to be effective COVID-19 therapies, and seem to have a closely related mechanism of action.
Recent Advances in Copper-Doped Titanium Implants
Authors: Wu Yuncheng , Zhou Hao , Zeng Ye , Xie Hongxing , Ma Dongxu , Wang Zhoucheng , Liang Hanfeng
Published: March 2022

Titanium (Ti) and its alloys have been extensively used as implant materials in clinical practice due to their high corrosion resistance, light weight and excellent biocompatibility. However, the insufficient intrinsic osteogenic capacity of Ti and its alloys impedes bone repair and regeneration, and implant-related infection or inflammation remains the leading cause of implant failure. Bacterial infections or inflammatory diseases constitute severe threats to human health. The physicochemical properties of the material are critical to the success of clinical procedures, and the doping of Cu into Ti implants has been confirmed to be capable of enhancing the bone repair/regeneration, angiogenesis and antibacterial capability. This review outlines the recent advances in the design and preparation of Cu-doped Ti and Ti alloy implants, with a special focus on various methods, including plasma immersion implantation, magnetron sputtering, galvanic deposition, microarc oxidation and sol-gel synthesis. More importantly, the antibacterial and mechanical properties as well as the corrosion resistance and biocompatibility of Cu-doped Ti implants from different methods are systematically reviewed, and their prospects and limitations are also discussed.
Ferromagnetism and Superconductivity in CaRuO3/YBa2Cu3O7-δ Heterostructures
Authors: Ionescu Alina , Ivan Ion , Locovei Claudiu , Onea Melania , Crisan Adrian , Soltan Soltan , Schütz Gisela , Albrecht Joachim
Published: March 2022

The deposition of a ferromagnetic layer can affect the properties of high-temperature superconductors underneath. We investigated the influence of ferromagnetic CaRuO3 on the properties of YBa2Cu3O7-x (YBCO) superconducting thin films when the layers are either in direct contact or separated by a barrier layer of 5 nm SrTiO3. Detailed measurements of the magnetic moment of the superconductor and ferromagnet as a function of temperature and magnetic field have been performed using SQUID magnetometry. Magnetometry and relaxation measurements show that the modification of the superconducting properties of YBCO strongly depends on the interaction with the ferromagnetic layer on top. The barrier layer has a significant impact on both the supercon-ducting properties of the YBCO film and the ferromagnetic ordering of CaRuO3. The physical properties mentioned above were discussed in correlation with the materials’ structure determined by XRD analysis.
Improving the Durability of Lime Finishing Mortars by Modifying Them with Silicic Acid Sol
Authors: Loganina Valentina , Davydova Olga , Fediuk Roman , Amran Mugahed , Klyuev Sergey , Klyuev Alexander , Sabitov Linar , Nabiullina Karina
Published: March 2022

Lime materials are in great demand for the restoration of the walls of historical buildings. However, lime coatings have insufficient resistance during operation. The purpose of this work was the modification of lime mortars with silicic acid sol in order to obtain more durable crystalline materials for construction purposes. A technology has been developed for obtaining a silica-containing additive, which consists in passing a liquid glass solution with a density of 1.053 kg/m3 through a cationic column and obtaining a silicic acid sol with a pH of 3–4 and a charge of (−) 0.053 V. The regeneration time and the amount of sol have been determined. Regularities of change in the radius of particles of silicic acid sol depending on age are determined. It is established that at an early age (up to 5 days), the radius of sol particles can be determined in accordance with the Rayleigh equation, and at a later age, in accordance with the Heller equation. The results of the calculation show that at the age of 1–5 days, the radius of the sol particles is 17.1–17.9 nm, and then the particles become coarser and the particle radius is 131.2–143 nm at the age of 19 days. The work of adhesion of silicic acid sol to lime and the heat of wetting are estimated. It is shown that the work of adhesion of water to lime is 28.9 erg/cm2, and that of the sol is 32.8 erg/cm2. The amount of heat Q released when lime is wetted with SiO2 sol is 15.0 kJ/kg, and when lime is wetted with water, it is 10.6 kJ/kg.
Correction: Zhang, H.; Ling, Z. Finite Element Modeling on Shear Performance of Grouted Stud Connectors for Steel–Timber Composite Beams. Materials 2022, 15, 1196
Authors: Zhang Henan , Ling Zhibin
Published: March 2022

The authors wish to make the following corrections to the published paper [...]
Rational Degree of Belief Ceases to Be Additive When the Dutch Book Argument Is Transported to a New Environment
Authors: Bamber Donald
Published: March 2022

The strength of a person’s beliefs can be measured by the buying and selling prices they offer on contingent promissory notes. Consider a promissory note contingent on a proposition; it pays off one unit of money if the proposition is true and nothing otherwise. The more strongly a person believes the proposition, the higher the minimum price would be at which they would sell it. The same would apply to the maximum purchase price. The well-known Dutch Book Argument claims that, if the person’s beliefs are rational, their buying/selling prices should combine additively, meaning that the price of a promissory note contingent on the disjunction of two incompatible propositions should be the sum of the prices of the promissory notes contingent on the individual incompatible propositions. This paper shows that the essence of the Dutch Book Argument is that rational belief is additive because money is additive. It is proved that, if the structure of the Dutch Book Argument is kept, but a nonadditive resource is substituted for money, then rational belief will follow a nonadditive combining rule. It is also shown how rational buying/selling prices behave when the pay-off amount of a contingent note changes.
A RUL Prediction Method of Small Sample Equipment Based on DCNN-BiLSTM and Domain Adaptation
Authors: Chen Wenbai , Chen Weizhao , Liu Huixiang , Wang Yiqun , Bi Chunli , Gu Yu
Published: March 2022

To solve the problem of low accuracy of remaining useful life (RUL) prediction caused by insufficient sample data of equipment under complex operating conditions, an RUL prediction method of small sample equipment based on a deep convolutional neural network—bidirectional long short-term memory network (DCNN-BiLSTM) and domain adaptation is proposed. Firstly, in order to extract the common features of the equipment under the condition of sufficient samples, a network model that combines the deep convolutional neural network (DCNN) and the bidirectional long short-term memory network (BiLSTM) was used to train the source domain and target domain data simultaneously. The Maximum Mean Discrepancy (MMD) was used to constrain the distribution difference and achieve adaptive matching and feature alignment between the target domain samples and the source domain samples. After obtaining the pre-trained model, fine-tuning was used to transfer the network structure and parameters of the pre-trained model to the target domain for training, perform network optimization and finally obtain an RUL prediction model that was more suitable for the target domain data. The method was validated on a simulation dataset of commercial modular aero-propulsion provided by NASA, and the experimental results show that the method improves the prediction accuracy and generalization ability of equipment RUL under cross-working conditions and small sample conditions.
Optimization of Ultrasonic-Assisted Extraction of Total Flavonoids from Abrus Cantoniensis (Abriherba) by Response Surface Methodology and Evaluation of Its Anti-Inflammatory Effect
Authors: Wu En-Yun , Sun Wen-Jing , Wang Ying , Zhang Ge-Yin , Xu Bai-Chang , Chen Xiao-Gang , Hao Kai-Yuan , He Ling-Zhi , Si Hong-Bin
Published: March 2022

Abrus cantoniensis is a Chinese herbal medicine with efficacy in clearing heat and detoxification, as well as relieving liver pain. The whole plant, except the seeds, can be used and consumed. Flavonoids have been found in modern pharmacological studies to have important biological activities, such as anti-inflammatory, antibacterial and antioxidant properties. The antibacterial and antioxidant bioactivities of the total flavonoids of Abrus cantoniensis (ATF) have been widely reported in national and international journals, but there are fewer studies on their anti-inflammatory effects. The present study focused on the optimization of the ultrasonic extraction process of ATF by response surface methodology and the study of its anti-inflammatory effects in vitro and in vivo. The results showed that the factors that had a great impact on the ATF extraction were the material-to-liquid ratio, ultrasonic extraction cycles and ethanol concentration. The best extraction process used a material-to-liquid ratio of 1:47, ultrasonic extraction cycles of 4 times, an ethanol concentration of 50%, an ultrasonic extraction time of 40 min and an ultrasonic power of 125 W. Under these conditions, the actual extraction rate of total flavonoids was 3.68%, which was not significantly different from the predicted value of 3.71%. In an in vitro anti-inflammatory assay, ATF was found to be effective in alleviating LPS (lipopolysaccharide)-induced inflammation in mouse peritoneal macrophages. In an in vivo anti-inflammatory assay, ATF was found to have a significant inhibitory effect on xylene-induced ear swelling in mice and cotton ball granuloma in mice, and the inhibitory effect was close to that of the positive control drug dexamethasone. This may provide a theoretical basis for the further development of the medicinal value of Abrus cantoniensis.
Analytical Performance of Clay Paste Electrode and Graphene Paste Electrode-Comparative Study
Authors: Skowron Ewelina , Spilarewicz-Stanek Kaja , Guziejewski Dariusz , Koszelska Kamila , Metelka Radovan , Smarzewska Sylwia
Published: March 2022

The analytical performance of the clay paste electrode and graphene paste electrode was compared using square wave voltammetry (SWV) and cyclic voltammetry (CV). The comparison was made on the basis of a paracetamol (PA) determination on both working electrodes. The influence of pH and SWV parameters was investigated. The linear concentration ranges were found to be 6.0 × 10−7–3.0 × 10−5 and 2.0 × 10−6–8.0 × 10−5 mol L−1 for clay paste electrode (ClPE) and graphene paste electrode (GrPE), respectively. The detection and quantification limits were calculated as 1.4 × 10−7 and 4.7 ×10−7 mol L−1 for ClPE and 3.7 × 10−7 and 1.2 × 10−6 mol L−1 for GrPE, respectively. Developed methods were successfully applied to pharmaceutical formulations analyses. Scanning electron microscopy and energy-dispersive X-ray spectroscopy were used to characterize ClPE and GrPE surfaces. Clay composition was examined with wavelength dispersive X-ray (WDXRF).
Forensic Discrimination of Differentially Sourced Animal Blood Using a Bottom-Up Proteomics Based MALDI MS Approach
Authors: Kennedy Katie , Cole Laura , Witt Matthias , Sealey Mark , Francese Simona
Published: March 2022

Recently published work has reported the development and application of a bottom-up proteomic approach to distinguish between human and animal blood (down to animal species level), by rapid screening using Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS). In that study, it was additionally observed that intravenous animal blood exhibits different spectral profiles from blood collected within the animal chest cavity as well as from the diluted blood collected within packets of meat. In this follow-up study we explored the resulting hypothesis that, depending on how blood is shed or collected, protein biomarker profiles vary to the extent of systematically permitting a distinction between possible sources of blood (for example, flesh wound versus packaged meat). This intelligence may be important in reconstructing the dynamics of the crime. The combination of statistical analysis and tandem mass spectrometry has yielded additional animal blood markers as well as confirming the ability to correctly determine the animal species from which blood derived, regardless of the retailer selling it (amongst the five investigated). These data confirm the initial hypothesis and demonstrate the opportunity for the proteomics-MALDI combined approach to provide additional intelligence to the investigation of violent crimes when examining blood evidence.
Single-Step Hydrolysis and Derivatization of Homocysteine Thiolactone Using Zone Fluidics: Simultaneous Analysis of Mixtures with Homocysteine Following Separation by Fluorosurfactant-Modified Gold Nanoparticles
Authors: Tsiasioti Apostolia , Zacharis Constantinos K. , Tzanavaras Paraskevas D.
Published: March 2022

Herein, we report a new automated flow method based on zone fluidics for the simultaneous determination of homocysteine and homocysteine thiolactone using fluorimetric detection (λext = 370 nm/λem = 480 nm). Homocysteine thiolactone is hydrolyzed on-line in alkaline medium (1 mol L−1 NaOH) to yield homocysteine, followed by reaction with o-phthalaldehyde in a single step. Derivatization is rapid without the need of elevated temperatures and stopped-flow steps, while specificity is achieved through a unique reaction mechanism in the absence of nucleophilic compounds. Mixtures of the analytes can be analyzed quantitatively after specific separation with fluorosurfactant-capped gold nanoparticles that are selectively aggregated by homocysteine, leaving the thiolactone analogue in solution. As low as 100 nmol L−1 of the analyte(s) can be quantified in aqueous solutions, while concentrations > 2 μmol L−1 can be analyzed in artificial and real urine matrix following 20-fold dilution. The percent recoveries ranged between 87 and 119%.
The Exploration of Chemokines Importance in the Pathogenesis and Development of Endometrial Cancer
Authors: Dobroch Jakub , Bojczuk Klaudia , Kołakowski Adrian , Baczewska Marta , Knapp Paweł
Published: March 2022

Endometrial cancer (EC) is one of the most frequent female malignancies. Because of a characteristic symptom, vaginal bleeding, EC is often diagnosed in an early stage. Despite that, some EC cases present an atypical course with rapid progression and poor prognosis. There have been multiple studies conducted on molecular profiling of EC in order to improve diagnostics and introduce personalized treatment. Chemokines—a protein family that contributes to inflammatory processes that may promote carcinogenesis—constitute an area of interest. Some chemokines and their receptors present alterations in expression in tumor microenvironment. CXCL12, which binds the receptors CXCR4 and CXCR7, is known for its impact on neoplastic cell proliferation, neovascularization and promotion of epidermal–mesenchymal transition. The CCL2–CCR2 axis additionally plays a pivotal role in EC with mutations in the LKB1 gene and activates tumor-associated macrophages. CCL20 and CCR6 are influenced by the RANK/RANKL pathway and alter the function of lymphocytes and dendritic cells. Another axis, CXCL10–CXCR3, affects the function of NK-cells and, interestingly, presents different roles in various types of tumors. This review article consists of analysis of studies that included the roles of the aforementioned chemokines in EC pathogenesis. Alterations in chemokine expression are described, and possible applications of drugs targeting chemokines are reviewed.
Biomimetic Radical Chemistry and Applications
Authors: Chatgilialoglu Chryssostomos
Published: March 2022

Some of the most interesting aspects of free radical chemistry that emerged in the last two decades are radical enzyme mechanisms, cell signaling cascades, antioxidant activities, and free radical-induced damage of biomolecules. In addition, identification of modified biomolecules opened the way for the evaluation of in vivo damage through biomarkers. When studying free radical-based chemical mechanisms, it is very important to establish biomimetic models, which allow the experiments to be performed in a simplified environment, but suitably designed to be in strict connection with cellular conditions. The 28 papers (11 reviews and 17 articles) published in the two Special Issues of Molecules on “Biomimetic Radical Chemistry and Applications (2019 and 2021)” show a remarkable range of research in this area. The biomimetic approach is presented with new insights and reviews of the current knowledge in the field of radical-based processes relevant to health, such as biomolecular damages and repair, signaling and biomarkers, biotechnological applications, and novel synthetic approaches.
Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols
Authors: Kargarpour Zahra , Panahipour Layla , Miron Richard J. , Gruber Reinhard
Published: March 2022

Liquid platelet-rich fibrin (PRF) is produced by fractionation of blood without additives that initiate coagulation. Even though liquid PRF is frequently utilized as a natural source of fibrinogen to prepare sticky bone, the concentration of fibrinogen and the overall amount of “clottable PRF” components have not been evaluated. To this aim, we prepared liquid PRF at 300, 700, and 2000 relative centrifugal force (RCF), for 8 min and quantified the fibrinogen levels by immunoassay. We report here that, independent of the RCF, the fibrinogen concentration is higher in the platelet-poor plasma (PPP) compared to the buffy coat (BC) fraction of liquid PRF and further decreases in the remaining red fraction. We then determined the weight of the clotted PRF fractions before and after removing the serum. The PPP and BC fractions consist of 10.2% and 25.3% clottable matrix suggesting that more than half of the weight of clottable BC is caused by cellular components. Our data provide insights into the distribution of fibrinogen in the different fractions of liquid PRF. These findings suggest that PPP is the main source of clottable fibrinogen, while the BC is more a cell source when it comes to the preparation of sticky bone.
Recent Advances in the Synthesis of Inorganic Materials Using Environmentally Friendly Media
Authors: Gontrani Lorenzo , Tagliatesta Pietro , Donia Domenica Tommasa , Bauer Elvira Maria , Bonomo Matteo , Carbone Marilena
Published: March 2022

Deep Eutectic Solvents have gained a lot of attention in the last few years because of their vast applicability in a large number of technological processes, the simplicity of their preparation and their high biocompatibility and harmlessness. One of the fields where DES prove to be particularly valuable is the synthesis and modification of inorganic materials—in particular, nanoparticles. In this field, the inherent structural inhomogeneity of DES results in a marked templating effect, which has led to an increasing number of studies focusing on exploiting these new reaction media to prepare nanomaterials. This review aims to provide a summary of the numerous and most recent achievements made in this area, reporting several examples of the newest mixtures obtained by mixing molecules originating from natural feedstocks, as well as linking them to the more consolidated methods that use “classical” DES, such as reline.
Angiotensin II Type I Receptor (AT1R): The Gate towards COVID-19-Associated Diseases
Authors: El-Arif George , Khazaal Shaymaa , Farhat Antonella , Harb Julien , Annweiler Cédric , Wu Yingliang , Cao Zhijian , Kovacic Hervé , Abi Khattar Ziad , Fajloun Ziad , Sabatier Jean-Marc
Published: March 2022

The binding of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein to its cellular receptor, the angiotensin-converting enzyme 2 (ACE2), causes its downregulation, which subsequently leads to the dysregulation of the renin–angiotensin system (RAS) in favor of the ACE–angiotensin II (Ang II)–angiotensin II type I receptor (AT1R) axis. AT1R has a major role in RAS by being involved in several physiological events including blood pressure control and electrolyte balance. Following SARS-CoV-2 infection, pathogenic episodes generated by the vasoconstriction, proinflammatory, profibrotic, and prooxidative consequences of the Ang II–AT1R axis activation are accompanied by a hyperinflammatory state (cytokine storm) and an acute respiratory distress syndrome (ARDS). AT1R, a member of the G protein-coupled receptor (GPCR) family, modulates Ang II deleterious effects through the activation of multiple downstream signaling pathways, among which are MAP kinases (ERK 1/2, JNK, p38MAPK), receptor tyrosine kinases (PDGF, EGFR, insulin receptor), and nonreceptor tyrosine kinases (Src, JAK/STAT, focal adhesion kinase (FAK)), and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. COVID-19 is well known for generating respiratory symptoms, but because ACE2 is expressed in various body tissues, several extrapulmonary pathologies are also manifested, including neurologic disorders, vasculature and myocardial complications, kidney injury, gastrointestinal symptoms, hepatic injury, hyperglycemia, and dermatologic complications. Therefore, the development of drugs based on RAS blockers, such as angiotensin II receptor blockers (ARBs), that inhibit the damaging axis of the RAS cascade may become one of the most promising approaches for the treatment of COVID-19 in the near future. We herein review the general features of AT1R, with a special focus on the receptor-mediated activation of the different downstream signaling pathways leading to specific cellular responses. In addition, we provide the latest insights into the roles of AT1R in COVID-19 outcomes in different systems of the human body, as well as the role of ARBs as tentative pharmacological agents to treat COVID-19.