bluuebunny/crossref_metadata_2025 huggingface dataset:
PFLOTRAN-SIP: A PFLOTRAN Module for Simulating Spectral-Induced Polarization of Electrical Impedance Data
Authors: Ahmmed Bulbul , Mudunuru Maruti Kumar , Karra Satish , James Scott C. , Viswanathan Hari , Dunbar John A.
Published: December 2020

Spectral induced polarization (SIP) is a non-intrusive geophysical method that collects chargeability information (the ability of a material to retain charge) in the time domain or its phase shift in the frequency domain. Although SIP is a temporal method, it cannot measure the dynamics of flow and solute/species transport in the subsurface over long times (i.e., 10–100 s of years). Data collected with the SIP technique need to be coupled with fluid flow and reactive-transport models in order to capture long-term dynamics. To address this challenge, PFLOTRAN-SIP was built to couple SIP data to fluid flow and solute transport processes. Specifically, this framework couples the subsurface flow and transport simulator PFLOTRAN and geoelectrical simulator E4D without sacrificing computational performance. PFLOTRAN solves the coupled flow and solute-transport process models in order to estimate solute concentrations, which were used in Archie’s model to compute bulk electrical conductivities at near-zero frequency. These bulk electrical conductivities were modified while using the Cole–Cole model to account for frequency dependence. Using the estimated frequency-dependent bulk conductivities, E4D simulated the real and complex electrical potential signals for selected frequencies for SIP. These frequency-dependent bulk conductivities contain information that is relevant to geochemical changes in the system. This study demonstrated that the PFLOTRAN-SIP framework is able to detect the presence of a tracer in the subsurface. SIP offers a significant benefit over ERT in the form of greater information content. It provided multiple datasets at different frequencies that better constrained the tracer distribution in the subsurface. Consequently, this framework allows for practitioners of environmental hydrogeophysics and biogeophysics to monitor the subsurface with improved resolution.
Femtosecond Laser-Induced Surface Modification of the Electrolyte in Solid Oxide Electrolysis Cells
Authors: Marquardt Tobias , Hollmann Jan , Gimpel Thomas , Schade Wolfgang , Kabelac Stephan
Published: December 2020

Electrolyte-supported solid oxide cells are often used for steam electrolysis. Advantages are high mechanical stability and a low degradation rate. The aim of this proof of concept study was to use a femtosecond laser to process the electrolyte of an electrolyte-supported solid oxide cell and evaluate the effect of this laser treatment on the electrochemical performance. The femtosecond laser treatment induces a macroscopic and a superimposed microscopic structure. It can be proven that the electrolyte remains gas tight and the electrochemical performance increases independently of the laser parameters. The initial area-specific resistance degradation during a constant current measurement of 200 h was reduced from 7.9% for a non-treated reference cell to 3.2% for one of the laser-treated cells. Based on electrochemical impedance measurements, it was found that the high frequency resistance of the laser-treated cells was reduced by up to 20% with respect to the reference cell. The impedance spectra were evaluated by calculating the distribution of relaxation times, and in advance, a novel approach was used to approximate the gas concentration resistance, which was related to the test setup and not to the cell. It was found that the low frequency polarization resistance was increased for the laser-treated cells. In total, the area-specific resistance of the laser-treated cells was reduced by up to 14%.
A Comprehensive Overview of Dynamic Line Rating Combined with Other Flexibility Options from an Operational Point of View
Authors: Erdinç F. Gülşen , Erdinç Ozan , Yumurtacı Recep , Catalão João P. S.
Published: December 2020

The need for flexibility in power system operation gradually increases regarding more renewable energy integration, load growth, etc., and the system operators already invest in this manner to enhance the power system operation. Besides, the power system has thermally sensitive assets such as lines, transformers, etc. that are normally operated under highly conservative static ratings. There is a growing trend in this regard to use the actual capacity of such assets dynamically under varying operating conditions leading to a dynamic thermal rating concept which is referred as dynamic line rating (DLR) approach specifically for lines. This study provides a comprehensive overview of existing perspectives on DLR and combination with other flexibility options from an operational point of view. Apart from the existing review studies more focused on implementation category of DLR concept, the concentration on more operational stage from the power system operation point of view leads the difference of this study compared to the mentioned studies. A categorization of the DLR implementation for either being sole or combined usage as a flexibility option is further realized. Besides, a geographically categorized analysis on existing practical evidence on DLR concept and implementations is also presented in this study.
Exploration of DG Placement Strategy of Microgrids via FMFO Algorithm: Considering Increasing Power Demand and Diverse DG Combinations
Authors: Wang Xiancheng , Srinivasan Thiruvenkadam , Kim Hyuntae , Ra In-ho
Published: December 2020

Distributed energy resource (DER) has been widely deployed, and distributed generation (DG) can complement the distribution system. Favorable DG deployment provides the grid-connected microgrid (MG) with stable voltage and reduces emission and power generation costs. DGs are considered distributed feeders, and MG is required to be operated under the optimal state. Reconfiguration is a practical approach to optimizing resource allocation. The optimal global solution is obtained via optimization algorithms. In this paper, three objectives are defined, namely, minimization of economic cost (ECC), emission cost (EMC), and voltage deviation (VD). Consequently, a fuzzy moth-flame optimization (FMFO) algorithm is proposed to coordinate the interests of multiple objectives. Moreover, the simulation is conducted based on the standard IEEE 33-bus radial distribution system (RDS), under which the impact of deployment of various DG type and quantity on the MG is explored. In particular, diverse DG combinations are tried under the increasing power demand, and a high-stable voltage strategy is proposed to meet the specific demands. The simulation results reveal that: (1) the DG type has a significant impact on ECC and EMC; (2) penetration level of DG shows a positive-like relationship with the MG stability; and (3) the proposed FMFO algorithm exhibits an efficient performance in convergence.
Chemical Impacts of Potential CO2 and Brine Leakage on Groundwater Quality with Quantitative Risk Assessment: A Case Study of the Farnsworth Unit
Authors: Xiao Ting , McPherson Brian , Esser Richard , Jia Wei , Dai Zhenxue , Chu Shaoping , Pan Feng , Viswanathan Hari
Published: December 2020

Potential leakage of reservoir fluids is considered a key risk factor for geologic CO2 sequestration (GCS), with concerns of their chemical impacts on the quality of overlying underground sources of drinking water (USDWs). Effective risk assessment provides useful information to guide GCS activities for protecting USDWs. In this study, we present a quantified risk assessment case study of an active commercial-scale CO2-enhanced oil recovery (CO2-EOR) and sequestration field, the Farnsworth Unit (FWU). Specific objectives of this study include: (1) to quantify potential risks of CO2 and brine leakage to the overlying USDW quality with response surface methodology (RSM); and (2) to identify water chemistry indicators for early detection criteria. Results suggest that trace metals (e.g., arsenic and selenium) are less likely to become a risk due to their adsorption onto clay minerals; no-impact thresholds based on site monitoring data could be a preferable reference for early groundwater quality evaluation; and pH is suggested as an indicator for early detection of a leakage. This study may provide quantitative insight for monitoring strategies on GCS sites to enhance the safety of long-term CO2 sequestration.
Toxicological Effects of Inorganic Nanoparticle Mixtures in Freshwater Mussels
Authors: Auclair Joelle , Turcotte Patrice , Gagnon Christian , Peyrot Caroline , Wilkinson Kevin J. , Gagné François
Published: December 2020

The toxicological effects of nanoparticles mixtures in aquatic organisms are poorly understood. The purpose of this study was to examine the tissue metal loadings and sublethal effects of silver (nAg), cerium oxide (nCeO), copper oxide (nCuO) and zinc oxide (nZnO) nanoparticles individually at 50 µg/L and in two mixtures to freshwater mussels Dreissena bugensis. The mixtures consisted of 12.5 µg/L of each nanoparticle (Mix50) and 50 µg/L of each nanoparticles (Mix200). After a 96-h exposure period, mussels were analyzed for morphological changes, air time survival, bioaccumulation, inflammation (cyclooxygenase or COX activity), lipid peroxidation (LPO), DNA strand breaks, labile Zn, acetylcholinesterase (AChE) and protein–ubiquitin levels. The data revealed that mussels accumulated the nanoparticles with nCeO and nAg were the least and most bioavailable, respectively. Increased tissue metal loadings were observed for nCeO and nCuO in mixtures, while no mixture effects were observed for nAg and nZnO. The weight loss during air emersion was lower in mussels exposed to nCuO alone but not by the mixture. On the one hand, labile Zn levels was increased with nZnO but returned to control values with the Mix50 and Mix200, suggesting antagonism. On the other hand, DNA strand breaks were reduced for both mixtures compared to controls or to the nanoparticles individually, suggesting potentiation of effects. The same was found for protein–ubiquitin levels, which were decreased by nCeO and nCuO alone but not when in mixtures, which increased their levels. In conclusion, the data revealed that the behavior and effects of nanoparticles were influenced by other nanoparticles where antagonist and potentiation interactions were identified.
Innovative Animal Manure Management for Environmental Protection, Improved Soil Fertility, and Crop Production
Authors: Ro Kyoung S. , Szogi Ariel A. , Sigua Gilbert C.
Published: December 2020

Traditionally, livestock manure has been used to provide nutrients for plant growth and to improve soil conditions [...]
Methylomes in Vegans versus Pescatarians and Nonvegetarians
Authors: Filippov Valery , Jaceldo-Siegl Karen , Eroshkin Alexey , Loskutov Vasiliy , Chen Xin , Wang Charles , Duerksen-Hughes Penelope J.
Published: December 2020

Epigenetic studies in animal models have demonstrated that diet affects gene regulation by altering methylation patterns. We interrogated methylomes in humans who have different sources of protein in their diet. We compared methylation of DNA isolated from buffy coat in 38 vegans, 41 pescatarians and 68 nonvegetarians. Methylation data were obtained using Infinium HumanMethylation450 arrays and analyzed using the Partek Genomic software. Differences in differentially methylated sites were small, though with the use of relaxed statistical tests we did identify diet-associated differences. To further test the validity of these observations, we performed separate and independent comparisons of the methylation differences between vegans and nonvegetarians, and between vegans and pescatarians. The detected differences were then examined to determine if they were enriched in specific pathways. Pathway analysis revealed enrichment of several specific processes, including homeobox transcription and glutamate transport. The detected differences in DNA methylation patterns between vegans, pescatarians, and nonvegetarians enabled us to identify 77 CpG sites that may be sensitive to diet and/or lifestyle, though high levels of individual-specific differences were also noted.
Variation and Genetic Parameters of Leaf Morphological Traits of Eight Families from Populus simonii × P. nigra
Authors: Ren Jingshan , Ji Xinyue , Wang Changhai , Hu Jianjun , Nervo Giuseppe , Li Jinhua
Published: December 2020

Leaf morphology in Populus L. varies extensively among sections, species and clones under strong genetic control. P. nigra L. (section Aigeiros), with large and triangular leaves, is a commercial forest tree of economic importance for fast growth and high yield in Europe. P. simonii Carr. (section Tacamahaca) with small land rhomboid ovate leaves performs cold and dry resistance/tolerance in the semi-arid region of Northern China. Leaf morphological traits could be used as early indicators to improve the efficiency of selection. In order to investigate the genetic variation pattern of leaf morphology traits, estimate breeding values (combining ability), as well as evaluate crossing combinations of parents, 1872 intersectional progenies from eight families (P. simonii × P. nigra) and their parents were planted with cuttings for the clonal replicate field trial in Northern China. Four leaf size traits (area, perimeter, length, width) and roundness were measured with leaf samples from the 1-year-old clonal plantation. Significant differences regarding leaf traits were found between and among three female clones of P. simonii from Inner Mongolia, China and six male clones of P. nigra from Casale Monferrato, Italy. The genetic variation coefficient, heritability and genetic variance component of most traits in male parents were greater than these of female parents. Heritability estimates of male and female parents were above 0.56 and 0.17, respectively. Plentiful leaf variations with normal and continuous distributions exited in the hybrid progenies among and within families with the genetic variation coefficient and heritability above 28.49 and 0.24, respectively. Heritability estimates showed that leaf area was the most heritable trait, followed by leaf width. The breeding value ranking of parents allowed us to select the parental clones for new crosses and extend the mating design. Two male parental clones (N430 and N429) had greater breeding values (general combining ability, GCA) of leaf size traits than other clones. The special combining ability (SCA) of the crossing combination between P. simonii cl. ZL-3 and P. nigra cl. N430 was greater than that of others. Eight putatively superior genotypes, most combined with the female parental clone ZL-3, can be selected for future testing under near-commercial conditions. Significant genetic and phenotypic correlations were found between five leaf morphology traits with the coefficients above 0.9, except for leaf roundness. The results showed that leaf morphology traits were under strong genetic control and the parental clones with high GCA and SCA effects could be utilized in heterosis breeding, which will provide a starting point for devising a new selection strategy of parents and progenies.
Comparative Analysis of Transaction Prices and the Values of Forest Properties in Poland
Authors: Gaca Radosław , Zygmunt Robert , Gluszak Michal
Published: December 2020

Research Highlights: In the paper, we explore systematic discrepancy between sale prices and values of forest properties in Poland. We argue that the systematic valuation bias found is partially caused by the simplified parametric appraisal methodology currently used in Poland. Background and Objectives: Most of the forests in Poland are state-owned, but in recent decades, the market for private forest properties has been dynamically growing. In the paper, we investigate the relations between the actual transaction prices, and the estimated value of forest properties in selected regions in Poland. We hypothesize that sale prices systematically deviate from valuations. An additional question arises regarding the determinants of forest property prices. We hypothesize that due to asymmetric information positive amenities are not fully capitalized in property prices in Poland. Materials and Methods: In the paper, we adopt two regression models used to investigate the valuation accuracy and bias. We test the hypothesis that valuations are unbiased estimates of transaction prices. Results: The results indicate that market prices for forest properties systematically differ from estimated values. Conclusions: Systematic deviation of forest property sales prices from market values may contribute to the imperfect information available to the market participants, especially when information is asymmetrically distributed between buyers and sellers. This may confirm the hypothesis that sellers are not fully aware of the advantages of the property being sold, and provide further explanations for large systematic differences between sales prices and valuations based on parametric valuation methods used in Poland.
Exploring the Impact of Lipid-Rich Food Industry Waste Carbon Sources on the Growth of Candida cylindracea DSM 2031
Authors: Zieniuk Bartłomiej , Mazurczak-Zieniuk Patrycja , Fabiszewska Agata
Published: December 2020

The aim of this study was to evaluate the possibility of using several lipid-rich food industry wastes in the culture medium on the growth of Candida cylindracea DSM 2031 yeast strain. Four lipid wastes from the food industry: waste fish oil, rancid ghee, waste pork lard, and waste duck processing oil were investigated. It has been shown in the laboratory scale that the above-mentioned wastes can be used to obtain biomass and produce lipolytic enzymes by the tested strain and the C. cylindracea extracellular lipase is not constitutive. High yields of biomass (12.84, 12.75, and 12.24 g/dm3) were obtained in media containing waste duck processing oil, olive oil, and waste pork lard, respectively. The highest lipolytic activity was obtained in the media containing waste fish oil and rancid ghee (0.050 and 0.047 U/cm3). During 192-h flask cultures the highest extracellular lipase activity and biomass yield were observed in the late logarithmic phase. The study showed that there is a potential for waste management to produce lipolytic enzymes or to produce yeast biomass. The use of waste substrates may contribute to lowering the costs of commercial production, and such a solution is part of the sustainable development strategy.
Brewer’s Spent Yeast (BSY), an Underutilized Brewing By-Product
Authors: Jaeger Alice , Arendt Elke K. , Zannini Emanuele , Sahin Aylin W.
Published: December 2020

The repurposing of by-products and the reduction of waste from food processing streams is an ever-increasing area of interest. Brewer’s spent yeast (BSY) is a prevalent by-product of the brewing industry. The spent yeast cells are removed at the end of the bulk fermentation. A small amount of it is used to start the next batch of fermentation; however, the majority of the spent yeast is discarded. This discarded yeast is high in nutrients, in particular proteins, vitamins and minerals, as well as containing functional and biologically active compounds such as polyphenols, antioxidants, β-glucans and mannoproteins. At present, BSY is mainly used in animal feed as a cheap and readily available source of protein. This review explores alternative, value-added applications for brewer’s spent yeast including nutritional ingredients, functional food additives as well as non-food applications. A major challenge in the utilization of BSY in food for human consumption is the high level of RNA. An excess of RNA in the diet can lead to an increase in uric acid in the bloodstream, potentially causing painful health conditions like gout. This issue can be overcome by RNA degradation and removal via additional treatment, namely heat treatment and enzymatic treatment. There is potential for the use of BSY ingredients in various food applications, including meat substitutes, bakery products and savory snacks.
Kluyveromyces marxianus: Current State of Omics Studies, Strain Improvement Strategy and Potential Industrial Implementation
Authors: Ha-Tran Dung Minh , Nguyen Trinh Thi My , Huang Chieh-Chen
Published: December 2020

Bioethanol is considered an excellent alternative to fossil fuels, since it importantly contributes to the reduced consumption of crude oil, and to the alleviation of environmental pollution. Up to now, the baker yeast Saccharomyces cerevisiae is the most common eukaryotic microorganism used in ethanol production. The inability of S. cerevisiae to grow on pentoses, however, hinders its effective growth on plant biomass hydrolysates, which contain large amounts of C5 and C12 sugars. The industrial-scale bioprocessing requires high temperature bioreactors, diverse carbon sources, and the high titer production of volatile compounds. These criteria indicate that the search for alternative microbes possessing useful traits that meet the required standards of bioethanol production is necessary. Compared to other yeasts, Kluyveromyces marxianus has several advantages over others, e.g., it could grow on a broad spectrum of substrates (C5, C6 and C12 sugars); tolerate high temperature, toxins, and a wide range of pH values; and produce volatile short-chain ester. K. marxianus also shows a high ethanol production rate at high temperature and is a Crabtree-negative species. These attributes make K. marxianus promising as an industrial host for the biosynthesis of biofuels and other valuable chemicals.
Effect of Geographical Indication Information on Consumer Acceptability of Cooked Aromatic Rice
Authors: Jarma Arroyo Sara E. , Hogan Victoria , Ahrent Wisdom Debra , Moldenhauer Karen A. K. , Seo Han-Seok
Published: December 2020

Geographical indication (GI) labeling is used to represent information about specific geographical origins of target products. This study aimed at determining the impact of GI information on sensory perception and acceptance of cooked aromatic rice samples. Ninety-nine participants evaluated cooked rice samples prepared using each of three aromatic rice varieties both with and without being provided with GI information. Participants rated the acceptance and intensity of the cooked rice samples in terms of appearance, aroma, flavor, texture, and overall liking, and also reported how important the GI information was to them. The results showed that consumers rated the cooked rice samples higher in appearance and overall liking when provided with GI information. Interestingly, participants who valued “state-of-origin” information more highly exhibited increased hedonic ratings of cooked rice samples when provided with GI information, but not when no GI information was given. Participants provided with GI information rated flavor or sweetness intensities of cooked aromatic rice samples closer to just-about-right than those without such information. This study provides empirical evidence about how GI information modulates sensory perception and acceptance of cooked aromatic rice samples. The findings will help rice industry, farmers, and traders better employ GI labeling to increase consumer acceptability of their rice products.
Monitoring Virgin Olive Oil Shelf-Life by Fluorescence Spectroscopy and Sensory Characteristics: A Multidimensional Study Carried Out under Simulated Market Conditions
Authors: Lobo-Prieto Ana , Tena Noelia , Aparicio-Ruiz Ramón , García-González Diego L. , Sikorska Ewa
Published: December 2020

The control of virgin olive oil (VOO) freshness requires new tools that reflect the diverse chemical changes that take place during the market period. Fluorescence spectroscopy is one of the techniques that has been suggested for controlling virgin olive oil (VOO) freshness during its shelf-life. However, a complete interpretation of fluorescence spectra requires analyzing multiple parameters (chemical, physical–chemical, and sensory) to evaluate the pace of fluorescence spectral changes under moderate conditions with respect to other changes impacting on VOO quality. In this work, four VOOs were analyzed every month with excitation–emission fluorescence spectra. The same samples were characterized with the concentration of fluorophores (phenols, tocopherols, chlorophyll pigments), physical–chemical parameters (peroxide value, K232, K270, free acidity), and sensory attributes (medians of defects and of the fruity attribute). From the six components extracted with parallel factor analysis (PARAFAC), two components were assigned to chlorophyll pigments and those assigned to tocopherols, phenols, and oxidation products were selected for their ability to discriminate between fresh and aged oils. Thus, the component assigned to oxidation products correlated with K270 in the range 0.80–0.93, while the component assigned to tocopherols–phenols correlated with the fruity attribute in the range 0.52–0.90. The sensory analysis of the samples revealed that the changes of these PARAFAC components occurred at the same time as, or even before, the changes of the sensory characteristics.
Biomanufacturing of Tomato-Derived Nanovesicles
Authors: Bokka Ramesh , Ramos Anna Paulina , Fiume Immacolata , Manno Mauro , Raccosta Samuele , Turiák Lilla , Sugár Simon , Adamo Giorgia , Csizmadia Tamás , Pocsfalvi Gabriella
Published: December 2020

Micro- and nano-sized vesicles (MVs and NVs, respectively) from edible plant resources are gaining increasing interest as green, sustainable, and biocompatible materials for the development of next-generation delivery vectors. The isolation of vesicles from complex plant matrix is a significant challenge considering the trade-off between yield and purity. Here, we used differential ultracentrifugation (dUC) for the bulk production of MVs and NVs from tomato (Solanum lycopersicum L.) fruit and analyzed their physical and morphological characteristics and biocargo profiles. The protein and phospholipid cargo shared considerable similarities between MVs and NVs. Phosphatidic acid was the most abundant phospholipid identified in NVs and MVs. The bulk vesicle isolates were further purified using sucrose density gradient ultracentrifugation (gUC) or size-exclusion chromatography (SEC). We showed that SEC using gravity column efficiently removed co-purifying matrix components including proteins and small molecular species. dUC/SEC yielded a high yield of purified vesicles in terms of number of particles (2.6 × 1015 particles) and protein quantities (6.9 ± 1.5 mg) per kilogram of tomato. dUC/gUC method separated two vesicle populations on the basis of buoyant density. Proteomics and in silico studies of the SEC-purified MVs and NVs support the presence of different intra- and extracellular vesicles with highly abundant lipoxygenase (LOX), ATPases, and heat shock proteins (HSPs), as well as a set of proteins that overlaps with that previously reported in tomato chromoplast.
A Comparative Study of the Bioavailability of Fe, Cu and Zn from Gluten-Free Breads Enriched with Natural and Synthetic Additives
Authors: Rogaska Anna , Reguła Julita , Suliburska Joanna , Krejpcio Zbigniew
Published: December 2020

The aim of this study was to compare the bioavailability of iron, copper and zinc from newly designed gluten-free breads enriched with natural and synthetic additives. The study was conducted on rats with induced Fe, Cu and Zn deficiency. The nutritional intervention with diets supplemented with a 70% addition of gluten-free breads enriched with natural additives and organic compounds to the control diet AIN-93M lasted 40 days. After the intervention, the rats were euthanized, the organs were collected and their mineral content was measured. Chemical analysis of diets with the addition of fortified gluten-free breads showed significantly higher amounts of iron, zinc and copper in diets with the addition of fortified breads compared to diets with the addition of unenriched breads. The type of additives did not influence the amount of minerals in diets. It is necessary to conduct further research to explain the interactions of ingredients and the factors affecting the bioavailability of Fe, Cu and Zn from gluten-free breads in order to obtain a product with a high bioavailability of these ingredients.
Genome-Wide Analysis of Off-Target CRISPR/Cas9 Activity in Single-Cell-Derived Human Hematopoietic Stem and Progenitor Cell Clones
Authors: Smith Richard H. , Chen Yun-Ching , Seifuddin Fayaz , Hupalo Daniel , Alba Camille , Reger Robert , Tian Xin , Araki Daisuke , Dalgard Clifton L. , Childs Richard W. , Pirooznia Mehdi , Larochelle Andre
Published: December 2020

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)-mediated genome editing holds remarkable promise for the treatment of human genetic diseases. However, the possibility of off-target Cas9 activity remains a concern. To address this issue using clinically relevant target cells, we electroporated Cas9 ribonucleoprotein (RNP) complexes (independently targeted to two different genomic loci, the CXCR4 locus on chromosome 2 and the AAVS1 locus on chromosome 19) into human mobilized peripheral blood-derived hematopoietic stem and progenitor cells (HSPCs) and assessed the acquisition of somatic mutations in an unbiased, genome-wide manner via whole genome sequencing (WGS) of single-cell-derived HSPC clones. Bioinformatic analysis identified >20,000 total somatic variants (indels, single nucleotide variants, and structural variants) distributed among Cas9-treated and non-Cas9-treated control HSPC clones. Statistical analysis revealed no significant difference in the number of novel non-targeted indels among the samples. Moreover, data analysis showed no evidence of Cas9-mediated indel formation at 623 predicted off-target sites. The median number of novel single nucleotide variants was slightly elevated in Cas9 RNP-recipient sample groups compared to baseline, but did not reach statistical significance. Structural variants were rare and demonstrated no clear causal connection to Cas9-mediated gene editing procedures. We find that the collective somatic mutational burden observed within Cas9 RNP-edited human HSPC clones is indistinguishable from naturally occurring levels of background genetic heterogeneity.
Trends and Characteristics of Emergency Medical Services in Italy: A 5-Years Population-Based Registry Analysis
Authors: Campagna Sara , Conti Alessio , Dimonte Valerio , Dalmasso Marco , Starnini Michele , Gianino Maria Michela , Borraccino Alberto
Published: December 2020

Background: Emergency Medical Services (EMS) plays a fundamental role in providing good quality healthcare services to citizens, as they are the first responders in distressing situations. Few studies have used available EMS data to investigate EMS call characteristics and subsequent responses. Methods: Data were extracted from the emergency registry for the period 2013–2017. This included call and rescue vehicle dispatch information. All relationships in analyses and differences in events proportion between 2013 and 2017 were tested against the Pearson’s Chi-Square with a 99% level of confidence. Results: Among the 2,120,838 emergency calls, operators dispatched at least one rescue vehicle for 1,494,855. There was an estimated overall incidence of 96 emergency calls and 75 rescue vehicles dispatched per 1000 inhabitants per year. Most calls were made by private citizens, during the daytime, and were made from home (63.8%); 31% of rescue vehicle dispatches were advanced emergency medical vehicles. The highest number of rescue vehicle dispatches ended at the emergency department (74.7%). Conclusions: Our data showed that, with some exception due to environmental differences, the highest proportion of incoming emergency calls is not acute or urgent and could be more effectively managed in other settings than in an Emergency Departments (ED). Better management of dispatch can reduce crowding and save hospital emergency departments time, personnel, and health system costs.
Could Cognitive Behavioural Therapy Be an Effective Treatment for Long COVID and Post COVID-19 Fatigue Syndrome? Lessons from the Qure Study for Q-Fever Fatigue Syndrome
Authors: Vink Mark , Vink-Niese Alexandra
Published: December 2020

An increasing number of young and previously fit and healthy people who did not require hospitalisation continue to have symptoms months after mild cases of COVID-19. Rehabilitation clinics are already offering cognitive behavioural therapy (CBT) as an effective treatment for long COVID and post-COVID-19 fatigue syndrome based on the claims that it is effective for myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)—the most common post-infectious syndrome—as no study into the efficacy of CBT for post-COVID-19 fatigue syndrome has been published. Re-analyses of these studies, however, showed that CBT did not lead to objective improvements in heterogeneous groups of ME/CFS patients, nor did it restore the ability to work. The group of patients with long COVID and post-COVID-19 fatigue syndrome, on the other hand, is homogeneous. We therefore analysed the Dutch Qure study, as it studied the efficacy of CBT in a homogeneous group of patients who developed Q-fever fatigue syndrome—which affects up to 30% of patients—after the largest reported outbreak of Q-fever, to see if CBT might potentially be an effective treatment for long-haulers after COVID-19 infection. Our reanalysis found that the Qure study suffered from many serious methodological problems, which included relying on one subjective primary outcome in a study without a control group for the non-blinded CBT treatment group, using a post hoc definition of improvement, waiting 2 years before publishing their objective actometer results and ignoring the null effect of said results. Moreover, only 10% of participants achieved a clinically meaningful subjective improvement in fatigue as a result of CBT according to the study’s own figures. Consequently, CBT has no subjective clinically meaningful effect in nine out of every ten patients that are treated with it. Additionally, the subjective improvement in fatigue was not matched by an improvement in disability, even though the disability was fatigue related according to the researchers. On top of this, CBT did not lead to an objective improvement in physical performance. Therefore, it cannot be said that CBT is an effective treatment for Q-fever fatigue syndrome either. It seems therefore unlikely that CBT will reduce disability or lead to objective improvement in long COVID or in post-COVID-19 fatigue syndrome.
Frontline Healthcare Workers’ Knowledge and Perception of COVID-19, and Willingness to Work during the Pandemic in Nepal
Authors: Upadhyaya Dipak Prasad , Paudel Rajan , Acharya Dilaram , Khoshnood Kaveh , Lee Kwan , Park Ji-Hyuk , Yoo Seok-Ju , Shrestha Archana , BC Bom , Bhandari Sabin , Yadav Ramgyan , Timalsina Ashish , Wagle Chetan Nidhi , Das Brij Kumar , Kunwar Ramesh , Chalise Binaya , Bhatta Deepak Raj , Adhikari Mukesh
Published: December 2020

This study investigated the contextual factors associated with the knowledge, perceptions, and the willingness of frontline healthcare workers (FHWs) to work during the COVID-19 pandemic in Nepal among a total of 1051 FHWs. Multivariable logistic regression analysis was applied to identify independent associations between predictors and outcome variables. Of the total study subjects, 17.2% reported inadequate knowledge on COVID-19, 63.6% reported that they perceived the government response as unsatisfactory, and 35.9% showed an unwillingness to work during the pandemic. Our analyses demonstrated that FHWs at local public health facilities, pharmacists, Ayurvedic health workers (HWs), and those with chronic diseases were less likely, and male FHWs were more likely, to have adequate knowledge of COVID-19. Likewise, nurses/midwives, public health workers, FHWs from Karnali and Far-West provinces, and those who had adequate knowledge of COVID-19 were more likely to have satisfactory perceptions towards the government response. Further, FHWs—paramedics, nurse/midwives, public health workers, laboratory workers—FHWs from Karnali Province and Far-West Province, and those with satisfactory perceptions of government responses to COVID-19 were predictors of willingness to work during the COVID-19 pandemic. These results suggest that prompt actions are required to improve FHWs’ knowledge of COVID-19, address negative perceptions of government responses, and motivate them through specific measures to provide healthcare services during the pandemic.
Transition of Serum Cytokine Concentration in Rickettsia japonica Infection
Authors: Kondo Makoto , Matsushima Yoshiaki , Mizutani Kento , Iida Shohei , Habe Koji , Yamanaka Keiichi
Published: December 2020

(1) Background. Rickettsia japonica (R. japonica) infection induces severe inflammation, and the disappearance of eosinophil in the acute stage is one of the phenomena. (2) Materials and Methods. In the current study, we measured the serum concentrations of Th1, Th2, and Th17 cytokines in the acute and recovery stages. (3) Results. In the acute phase, IL-6 and IFN-γ levels were elevated and we speculated that they played a role as a defense mechanism against R. japonica. The high concentration of IFN-γ suppressed the differentiation of eosinophil and induced apoptosis of eosinophil, leading to the disappearance of eosinophil. On day 7, IL-6 and IFN-γ concentrations were decreased, and Th2 cytokines such as IL-5 and IL-9 were slightly increased. On day 14, eosinophil count recovered to the normal level. The transition of serum cytokine concentration in R. japonica infection was presented. (4) Conclusions. IL-6 and IFN-γ seem to be critical cytokines as defense mechanism against R. japonica in the acute phase, and this may deeply connect to the decrease of eosinophil.
Functional Analysis of the Spine with the Idiag SpinalMouse System among Sedentary Workers Affected by Non-Specific Low Back Pain
Authors: Csuhai Éva Anett , Nagy Attila Csaba , Váradi Zsuzsanna , Veres-Balajti Ilona
Published: December 2020

WHO describes “low back pain” (LBP) as the most common problem in overall occupational-related diseases. The aim of this study was to evaluate characteristics of spinal functionality among sedentary workers and determine usability of the SpinalMouse® skin-surface measurement device in workplace settings in a risk population for LBP. The spinal examination was implemented at National Instruments Corporations’ Hungarian subsidiary, Debrecen in October, 2015, involving 95 white-collar employees as volunteers to assess spinal posture and functional movements. Data from the physical examination of 91 subjects (age: 34.22 ± 7.97 years) were analyzed. Results showed significant differences (p < 0.05) in posture and mobility of the spinal regions in sitting compared to standing position. Significant positive correlations were observed between values measured in standing and sitting positions in all observed regions and aspects of the spine (p < 0.05) except posture of lumbar extension (p = 0.07) and mobility of sacrum/hip in E-F (p = 0.818). Significant (p < 0.001) difference (5.70°) was found between the spinal inclination in sitting 6.47 ± 3.55° compared to standing 0.77 ± 2.53 position. Sitting position has a negative effect on the posture and mobility of the spine among white-collar employees. The SpinalMouse can be used effectively to determine spinal posture and mobility in cross-sectional studies and impact analysis of physical exercise interventions.
Supporting a Healthier Takeaway Meal Choice: Creating a Universal Health Rating for Online Takeaway Fast-Food Outlets
Authors: Goffe Louis , Uwamahoro Nadege S. , Dixon Christopher J. , Blain Alasdair P. , Danielsen Jona , Kirk David , Adamson Ashley J.
Published: December 2020

Digital food ordering platforms are used by millions across the world and provide easy access to takeaway fast-food that is broadly, though not exclusively, characterised as energy dense and nutrient poor. Outlets are routinely rated for hygiene, but not for their healthiness. Nutritional information is mandatory in pre-packaged foods, with many companies voluntarily using traffic light labels to support making healthier choices. We wanted to identify a feasible universal method to objectively score takeaway fast-food outlets listed on Just Eat that could provide users with an accessible rating that can infer an outlet’s ‘healthiness’. Using a sample of takeaway outlets listed on Just Eat, we obtained four complete assessments by nutrition researchers of each outlet’s healthiness to create a cumulative score that ranged from 4 to 12. We then identified and manually extracted nutritional attributes from each outlet’s digital menu, e.g., number of vegetables that have the potential to be numerated. Using generalized linear modelling we identified which attributes were linear predictors of an outlet’s healthiness assessment from nutritional researchers. The availability of water, salad, and the diversity of vegetables were positively associated with academic researchers’ assessment of an outlet’s healthiness, whereas the availability of chips, desserts, and multiple meal sizes were negatively associated. This study shows promise for the feasibility of an objective measure of healthiness that could be applied to all outlet listings on Just Eat and other digital food outlet aggregation platforms. However, further research is required to assess the metric’s validity, its desirability and value to users, and ultimately its potential influence on food choice behaviour.
What Is the Impact of a Context-Specific Training Program for Home-Based Carers? An Evaluation Study
Authors: Bopape Mamare Adelaide , Mothiba Tebogo Maria , Bastiaens Hilde , Wens Johan
Published: December 2020

Introduction: In South Africa (SA), home-based carers (HBCs) play a crucial role at the community level for non-communicable diseases (NCDs) including diabetes mellitus (DM) public health care. The work of HBCs requires them to be knowledgeable about diabetes, and lack of knowledge has implications on their roles for the provision of health information and dietary advice. HBCs need to be provided with specific training to develop their knowledge and skills necessary to enable them to provide care to people with diabetes (PWD) because organizations need to benefit from a pool of well-trained HBCs. Therefore, a training program was developed to improve care for chronic conditions based on local needs. Aim: To implement and evaluate the training program for the HBCs for PWD in Ga-Dikgale village. Methods: HBCs working at Ga- Dikgale villages in four clinics—namely, Dikgale, Seobi Dikgale, Sebayeng, and Makotopong—participated voluntarily. Fifty-five (55) HBCs who attended the training program completed satisfaction survey tools, and furthermore, 45 HBCs completed both pre-training and post-training knowledge questionnaires. Training divided into two sessions which each lasted for two days was conducted. Satisfaction with the training, improvement of knowledge, and perceived impact on daily practice were evaluated using both qualitative and quantitative approaches. Results: Quantitative results indicate that 72% had poor knowledge of pre-training and only 9% post-training. They scored more in a post-test with the following differences: Post-test (mean = 6.00, SD = 1.26); pre-test (mean = 3.31, SD = 1.77). The t-test results indicated the difference to be significant, t = −9.241, p = 0.000. From the qualitative results, the themes that emerged during data analysis from group discussions were HBCs’ achievements from the training, challenges related to diabetes and diet, and suggestions for further training. Conclusions: A context-specific training increased diabetes knowledge among the HBCs for PWD. The results highlighted the importance of training in improving the knowledge of HBCs about the care of PWD. The improvement in diabetes knowledge among HBCs needs to be maintained and sustained to achieve major health benefits for PWD.
A Longitudinal Analysis of Gambling Predictors among Adolescents
Authors: Botella-Guijarro Álvaro , Lloret-Irles Daniel , Segura-Heras José Vicente , Cabrera-Perona Víctor , Moriano Juan Antonio
Published: December 2020

Although gambling is forbidden for minors, the prevalence of gambling among adolescents is increasing. In order to improve preventive interventions, more evidence on predictors of gambling onset is needed. A longitudinal study was proposed to (1) establish the prevalence of gambling; (2) identify factors associated with gambling behavior the following year; and (3) adjust a model to predict gambling behavior. A cohort of 1074 students (13–18 years old) was followed for 12 months. The prevalence of gambling reached 42.0% in the second measure. Boys gambled 2.7 times more than girls, and the highest percentages of gambling onset showed up between 13 and 14 years old. Gambling onset and maintenance was associated with gender, age, sensation-seeking, risk perception, self-efficacy for not gambling, parents’ attitude towards gambling, group pressure (friends), subjective norm, exposure to advertising, accessibility, normative perception, gambling in T1 and parents gambling behavior. Gender, gambling in T1 and risk perception were significant in all three logistic adjusted regression models, with the fourth variable being sensation seeking, peer pressure (friends) and accessibility, respectively. It is suggested that universal prevention should be aimed preferably at children under 15 years old and to alert regulators and public administrations to the directly proportional relationship between accessibility and gambling onset.
The Assessment of Radon Emissions as Results of the Soil Technogenic Disturbance
Authors: Leshukov Timofey , Larionov Aleksey , Legoshchin Konstantin , Lesin Yuriy , Yakovleva Svetlana
Published: December 2020

222Rn is a specific indoor-type pollutant that represents a primary radiological hazard as a main source of ionizing radiation (IR) for humans. Coal mining creates new sources of gas that are formed over mines. This process can significantly increase the density of radon flux. Therefore, the concentration of radon in a room can increase. We investigated the territory of the Leninsk-Kuznetsky district of the Kemerovo region, which is subject to underground mining. Two groups of residential locations and measuring points of radon flux density were selected to identify the higher emanation relationship of radon and mining-affected areas. The first group (Case group) included subjects located within the territory of the underground mine; the other (Control group) included subjects in an area without mining. Radon flux density in coal mining areas was significantly higher than in the rest of the territory; moreover, the percentage of values in the Case group that had a radon flux density above 80 mBq·m−2·s−1 was 64.53%. For the Case group, 20.62% of residential buildings had a radon concentration above 200 Bq/m3. For the studied area, the radon flux density correlates positively (r = 0.79, p = 0.002) with indoor radon. Additional clastogenic/aneugenic effects are also found in dwellings with increased volume activity of radon (VAR) within the territories of underground mines. Ring chromosomes are positively correlated with radon levels in smoker groups but not in non-smokers. An increased frequency of binucleated (BN) cells with micronuclei (MN) is also positively correlated with VAR regardless of smoking status. It has been concluded that reducing the total exposure level of a population to radon can be achieved by monitoring areas with underground mines where radon is emitted heavily.