bluuebunny/crossref_metadata_2025 huggingface dataset:
Reforms and innovations in primary health care in different countries: scoping review
Authors: Azimzadeh Solmaz , Azami-Aghdash Saber , Tabrizi Jafar Sadegh , Gholipour Kamal
Published: April 2024

Introduction:

The World Health Organization (WHO) recommends focusing on primary health care (PHC) as the first strategy of countries to achieve the improvement of the health level of communities and has emphasized it again in 2021. Therefore, we intend to take a different look at the PHC system with reform, innovation, and initiative by using the experiences of leading countries and identify practical and evidence-based solutions to achieve greater health.

Methods:

This is a scoping review study that has identified innovations and reforms related to PHC since the beginning of 2000 to the end of 2022. In this study, Scopus, Web Of Science, and PubMed databases have been searched using appropriate keywords. This study is done in six steps using Arkesy and O’Malley framework. In this study, the framework of six building blocks of WHO was used to summarize and report the findings.

Results:

By searching in different databases, we identified 39426 studies related to reforms in primary care, and after the screening process, 106 studies were analyzed. Our findings were classified and reported into 9 categories (aims, stewardship/leadership, financing & payment, service delivery, health workforce, information, outcomes, policies/considerations, and limitations).

Conclusion:

The necessity and importance of strengthening PHC is obvious to everyone due to its great consequences, which requires a lot of will, effort, and coordination at the macro-level of the country, various organizations, and health teams, as well as the participation of people and society.
Evaluating the Role of Titanomagnetite in Bubble Nucleation: Novel Applications of Low Temperature Magnetic Analysis and Textural Characterization of Rhyolite Pumice and Obsidian From Glass Mountain, California
Authors: McCartney Kelly N. , Hammer Julia E. , Shea Thomas , Brachfeld Stefanie , Giachetti Thomas
Published: April 2024

Nucleation of H2O vapor bubbles in magma requires surpassing a chemical supersaturation threshold via decompression. The threshold is minimized in the presence of a nucleation substrate (heterogeneous nucleation, <50 MPa), and maximized when no nucleation substrate is present (homogeneous nucleation, >100 MPa). The existence of explosively erupted aphyric rhyolite magma staged from shallow (<100 MPa) depths represents an apparent paradox that hints at the presence of a cryptic nucleation substrate. In a pair of studies focusing on Glass Mountain eruptive units from Medicine Lake, California, we characterize titanomagnetite nanolites and ultrananolites in pumice, obsidian, and vesicular obsidian (Brachfeld et al., 2024, https://doi.org/10.1029/2023GC011336), calculate titanomagnetite crystal number densities, and compare titanomagnetite abundance with the physical properties of pumice to evaluate hypotheses on the timing of titanomagnetite crystallization. Titanomagnetite crystals with grain sizes of approximately 3–33 nm are identified in pumice samples from the thermal unblocking of low‐temperature thermoremanent magnetization. The titanomagnetite number densities for pumice are 1018 to 1020 m−3, comparable to number densities in pumice and obsidian obtained from room temperature methods (Brachfeld et al., 2024, https://doi.org/10.1029/2023GC011336). This range exceeds reported bubble number densities (BND) within the pumice from the same eruptive units (average BND ∼4 × 1014 m−3). The similar abundances of nm‐scale titanomagnetite crystals in the effusive and explosive products of the same eruption, together with the lack of correlation between pumice permeability and titanomagnetite content, are consistent with titanomagnetite formation having preceded the bubble formation. Results suggest sub‐micron titanomagnetite crystals are responsible for heterogeneous bubble nucleation in this nominally aphyric rhyolite magma.
Middle Neoproterozoic (Tonian) Polar Wander of South China: Paleomagnetism and ID‐TIMS U‐Pb Geochronology of the Laoshanya Formation
Authors: Tonti‐Filippini Justin A. D. , Robert Boris , Muller Élodie , Paul André N. , Dellefant Fabian , Wack Michael , Meng Jun , Zhao Xixi , Schaltegger Urs , Gilder Stuart A.
Published: April 2024

Paleomagnetic records of middle Neoproterozoic (820 to 780 Ma) rocks display high amplitude directional variations that lead to large discrepancies in paleogeographic reconstructions. Hypotheses to explain these data include rapid true polar wander (TPW), a geomagnetic field geometry that deviates from a predominantly axial dipole field, a hyper‐reversing field (>10 reversals/Ma), and/or undiagnosed remagnetization. To test these hypotheses, we collected 1,057 oriented cores over a 85 m stratigraphic succession in the Laoshanya Formation (Yangjiaping, Hunan, China). High precision U‐Pb dating of two intercalated tuff layers constrain the age of the sediments between 809 and 804 Ma. Thermal demagnetization isolates three magnetization components residing in hematite which are not time‐progressive but conflated throughout the section. All samples possess a north and downward directed component in geographic coordinates at temperatures up to 660°C that is ascribed to a Cretaceous overprint. Two components isolated above 660°C reveal distinct directional clusters: one is interpreted as a depositional remanence, while the other appears to be the result of a mid‐Paleozoic (460 to 420 Ma) remagnetization, which is likely widespread throughout South China. The high‐temperature directions are subtly dependent on lithology; microscopic and rock magnetic analyses identify multiple generations of hematite that vary in concentration and distinguish the magnetization components. A comparison with other middle Neoproterozoic paleomagnetic studies in the region indicates that the sudden changes in paleomagnetic directions, used elsewhere to support the rapid TPW hypothesis (ca. 805 Ma), are better explained by mixtures of primary and remagnetized components, and/or vertical axis rotations.
Deciphering Clues Regarding Magma Composition Encoded in Quartz‐Hosted Embayments and Melt Inclusions Through Direct Numerical Simulations
Authors: Wei Zihan , Ruefer Anna C. , Pamukcu Ayla S. , Suckale Jenny
Published: April 2024

Crystal‐hosted melt embayments and melt inclusions partially record magmatic processes at depth, but it is not always obvious how to interpret this record. One impediment is our incomplete understanding of how embayments and melt inclusions form. In this study, we investigate the formation mechanism of embayments and melt inclusions during quartz growth to quantify the relationship between the compositions of the entrapped and average melt. We study the growth of embayments and inclusions through direct numerical simulations that couple the growth of a crystal surface with the evolution of the concentrations of incompatible components in the surrounding melt. We find that H2O is more enriched in the interior of defects on crystal surface compared to the exterior. The resultant lower disequilibrium in the defect interior causes lower growth rate than in the exterior, elongating the defect into an embayment. If crystal growth stops, the composition in the embayment equilibrates with the average melt within days to months. If crystal growth continues until the embayment neck closes, a melt inclusion forms. The melt entrapped by both embayments and melt inclusions is enriched in incompatible components, such as H2O and CO2. In addition to inclusion size, the enrichment of incompatible components in melt inclusions also depends on component diffusivity and the crystal growth regime. High‐diffusivity components like H2O have similar enrichment levels in all scenarios, while lower‐diffusivity components like CO2 are more enriched in melt inclusions with smaller sizes or formed in continuous crystal growth.
Insights Into the Structure of the Mongol‐Okhotsk Suture Zone, Adaatsag Ophiolite, and Tectonic Boundaries of the Central Asian Orogenic Belt (Mongolia) From Electrical Resistivity Imaging and Seismic Velocity Models
Authors: Comeau Matthew J. , Rigaud Rafael , Batmagnai Erdenechimeg , Tserendug Shoovdor , Kuvshinov Alexey , Becken Michael , Demberel Sodnomsambuu
Published: April 2024

The Mongol‐Okhotsk suture and the Adaatsag ophiolite belt are associated with the closure of the Mongol‐Okhotsk paleo‐ocean and are located within the Central Asian Orogenic Belt (CAOB) and Mongolia. The suture zone is flanked by volcanic‐plutonic belts that host significant metallogenic zones, containing deposits of copper and gold. The tectonic evolution of this region is not fully understood and the lithospheric structure has been poorly studied. We analyze magnetotelluric data and generate a model of the electrical resistivity distribution across this region. Whereas the northern segment has a sharp transition from a high‐resistivity upper crust to a low‐resistivity lower crust, as observed beneath the Hangai Dome, the southern segment does not show this transition. A wide, low‐resistivity zone (1–100 Ωm) imaged in the crust and lithospheric mantle is coincident with the Mongol‐Okhotsk suture and ophiolite, revealing a clear and significant lithospheric‐scale feature. Across the profile, numerous narrow, vertically oriented, low‐resistivity features (1–100 Ωm) are spatially associated remarkably well with the proposed boundaries of tectonic domains. These results confirm ideas about the development of the CAOB. Some of these low‐resistivity features are beneath the surface locations of large mineral zones, and likely represent fossil fluid pathways. We show congruent seismic velocity models for comparison and the results show a large‐scale low‐velocity anomaly (decrease of 2%–3%) that correlates with the location of the low‐resistivity anomaly below the Mongol‐Okhotsk suture. The geophysical results, combined with geological and geochemical data, provide insights into the structure of this region and help shed light on unanswered questions.
Revisiting the Tianwen Yellow Pumice (TYP) Eruption of Changbaishan Volcano: Tephra Correlation, Eruption Timing and Its Climatostratigraphical Context
Authors: Chen Xuan‐Yu , Xu Yi‐Gang , Tarasov Pavel E. , Leipe Christian , Kim Ji‐Hoon , Yan Shuang , Park Myong‐Ho , Chun Jong‐Hwa , Chen Cong , He Peng‐Li , Blockley Simon P. E.
Published: April 2024

Changbaishan volcano (China/North Korea) is one of the most active and hazardous volcanic centers in Northeast Asia. Despite decades of intensive research, the eruption history of this stratovolcano remains poorly constrained. One of the major puzzles is the timing of the eruption that produced the Tianwen Yellow Pumice (TYP) deposit at the caldera rim. Here we identify a new cryptotephra layer in sediment core 13PT‐P4 from the East Sea. Grain‐specific major, minor, and trace element analyses of glass shards allow a clear correlation of this distal tephra to the proximal TYP deposit of Changbaishan. Age‐depth modeling using radiocarbon (14C) dates of sediment bulk organic fractions and other tephrochronological markers from the sediment sequence constraints the age of the cryptotephra and thus the TYP eruption to 29,948–29,625 cal yr BP (95.4% confidence interval). Our findings lead to a revision of the history of Changbaishan explosive activity, and show that the volcano has been particularly active during ca. 51–24 ka BP in the last 100 ka. Using high resolution palaeo‐proxy records, we find the TYP tephra almost coeval with regional to hemispheric‐scale climatic changes known as Heinrich Event 3 (H3). With its precise age determination and wide geographic dispersion, the tephra offers a key isochron for dating records of past climatic changes and addressing the phasing relationships in environmental response to H3 across East Asia.
Three‐Dimensional Modeling of the O2(1∆) Dayglow: Dependence on Ozone and Temperatures
Authors: Diouf Mouhamadou Makhtar Ndiaga , Lefèvre Franck , Hauchecorne Alain , Bertaux Jean‐Loup
Published: April 2024

Future space missions dedicated to measuring CO2 on a global scale can make advantageous use of the O2 band at 1.27 μm to retrieve the air column. The 1.27 μm band is close to the CO2 absorption bands at 1.6 and 2.0 μm, which allows a better transfer of the aerosol properties than with the usual O2 band at 0.76 μm. However, the 1.27 μm band is polluted by the spontaneous dayglow of the excited state O2 (1∆), which must be removed from the observed signal. We investigate here our quantitative understanding of the O2(1∆) dayglow with a chemistry‐transport model. We show that the previously reported −13% deficit in O2(1∆) dayglow calculated with the same model is essentially due a −20% to −30% ozone deficit between 45 and 60 km. We find that this ozone deficit is due to excessively high temperatures (+15 K) of the meteorological analyses used to drive the model in the mesosphere. The use of lower analyzed temperatures (ERA5), in better agreement with the observations, slows down the hydrogen‐catalyzed and Chapman ozone loss cycles. This effect leads to an almost total elimination of the ozone and O2(1∆) deficits in the lower mesosphere. Once integrated vertically to simulate a nadir measurement, the deficit in modeled O2(1∆) brightness is reduced to −4.2 ± 2.8%. This illustrates the need for accurate mesospheric temperatures for a priori estimations of the O2(1∆) brightness in algorithms using the 1.27 μm band.
Empirical Models of foF2 and hmF2 Reconstituted by Global Ionosonde and Reanalysis Data and COSMIC Observations
Authors: Huang Fuqing , Ruan Haibing , Lei Jiuhou , Zhong Jiahao , Yue Xinan , Li Guozhu , Chen Yiding , He Jianhui , Li Na , Luan Xiaoli , Xiong Chao , Dou Xiankang
Published: April 2024

The F2‐peak plasma frequency (foF2) and the height of the F2 peak (hmF2) are two of the most important parameters for any ionospheric model, as well as radio propagation studies and applications. In this study, we have developed empirical models to capture the most significant variations of foF2 and hmF2. The derived empirical models (referred to as the USTC models within this study) are specified through global ionosonde and reanalysis data based on the International Reference Ionosphere (IRI) Consultative Committee on International Radio (CCIR) method and Constellation Observindg System for Meteorology, Ionosphere, and Climate (COSMIC) observations based on the empirical orthogonal function analysis, respectively. The USTC models are validated against the IRI CCIR model prediction. The comparison results revealed that the empirical foF2 model performs better in capturing the foF2 variations than the IRI CCIR model, which can overcome the underestimation of the IRI CCIR model at low latitudes. Although the IRI CCIR model overestimation at middle latitudes is addressed by the empirical hmF2 model, the visible differences between the model predictions and ionosonde observations still exist at low latitudes, which could be attributed to the significant difference between COSMIC and ionosonde hmF2 measures.
Developing a geographical–meteorological indicator system and evaluating prediction models for alveolar echinococcosis in China
Authors: Xue Chuizhao , Liu Baixue , Kui Yan , Wu Weiping , Zhou Xiaonong , Xiao Ning , Han Shuai , Zheng Canjun
Published: April 2024

Background

Geographical and meteorological factors have been reported to influence the prevalence of echinococcosis, but there’s a lack of indicator system and model.

Objective

To provide further insight into the impact of geographical and meteorological factors on AE prevalence and establish a theoretical basis for prevention and control.

Methods

Principal component and regression analysis were used to screen and establish a three-level indicator system. Relative weights were examined to determine the impact of each indicator, and five mathematical models were compared to identify the best predictive model for AE epidemic levels.

Results

By analyzing the data downloaded from the China Meteorological Data Service Center and Geospatial Data Cloud, we established the KCBIS, including 50 basic indicators which could be directly obtained online, 15 characteristic indicators which were linear combination of the basic indicators and showed a linear relationship with AE epidemic, and 8 key indicators which were characteristic indicators with a clearer relationships and fewer mixed effects. The relative weight analysis revealed that monthly precipitation, monthly cold days, the difference between negative and positive temperature anomalies, basic air temperature conditions, altitude, the difference between positive and negative atmospheric pressure anomalies, monthy extremely hot days, and monthly fresh breeze days were correlated with the natural logarithm of AE prevalence, with sequential decreases in their relative weights. The multinomial logistic regression model was the best predictor at epidemic levels 1, 3, 5, and 6, whereas the CART model was the best predictor at epidemic levels 2, 4, and 5.
Fatty acid-binding protein 5 is a functional biomarker and indicator of ferroptosis in cerebral hypoxia
Authors: Peng Hao , Xin Shan , Pfeiffer Susanne , Müller Constanze , Merl-Pham Juliane , Hauck Stefanie M. , Harter Patrick N. , Spitzer Daniel , Devraj Kavi , Varynskyi Borys , Arzberger Thomas , Momma Stefan , Schick Joel A.
Published: April 2024

The progression of human degenerative and hypoxic/ischemic diseases is accompanied by widespread cell death. One death process linking iron-catalyzed reactive species with lipid peroxidation is ferroptosis, which shows hallmarks of both programmed and necrotic death in vitro. While evidence of ferroptosis in neurodegenerative disease is indicated by iron accumulation and involvement of lipids, a stable marker for ferroptosis has not been identified. Its prevalence is thus undetermined in human pathophysiology, impeding recognition of disease areas and clinical investigations with candidate drugs. Here, we identified ferroptosis marker antigens by analyzing surface protein dynamics and discovered a single protein, Fatty Acid-Binding Protein 5 (FABP5), which was stabilized at the cell surface and specifically elevated in ferroptotic cell death. Ectopic expression and lipidomics assays demonstrated that FABP5 drives redistribution of redox-sensitive lipids and ferroptosis sensitivity in a positive-feedback loop, indicating a role as a functional biomarker. Notably, immunodetection of FABP5 in mouse stroke penumbra and in hypoxic postmortem patients was distinctly associated with hypoxically damaged neurons. Retrospective cell death characterized here by the novel ferroptosis biomarker FABP5 thus provides first evidence for a long-hypothesized intrinsic ferroptosis in hypoxia and inaugurates a means for pathological detection of ferroptosis in tissue.
An isolable, chelating bis[cyclic (alkyl)(amino)carbene] stabilizes a strongly bent, dicoordinate Ni(0) complex
Authors: Puerta Lombardi Braulio M. , Faas Morgan R. , West Daniel , Suvinen Roope A. , Tuononen Heikki M. , Roesler Roland
Published: April 2024

Chelating ligands have had a tremendous impact in coordination chemistry and catalysis. Notwithstanding their success as strongly σ-donating and π-accepting ligands, to date no chelating bis[cyclic (alkyl)(amino)carbenes] have been reported. Herein, we describe a chelating, C2-symmetric bis[cyclic (alkyl)(amino)carbene] ligand, which was isolated as a racemic mixture. The isolation and structural characterization of its isostructural, pseudotetrahedral complexes with iron, cobalt, nickel, and zinc dihalides featuring eight-membered metallacycles demonstrates the binding ability of the bis(carbene). Reduction of the nickel(II) dibromide with potassium graphite produces a dicoordinate nickel(0) complex that features one of the narrowest angles measured in any unsupported dicoordinate transition metal complexes.
Highly defective ultra-small tetravalent MOF nanocrystals
Authors: Dai Shan , Simms Charlotte , Patriarche Gilles , Daturi Marco , Tissot Antoine , Parac-Vogt Tatjana N. , Serre Christian
Published: April 2024

The size and defects in crystalline inorganic materials are of importance in many applications, particularly catalysis, as it often results in enhanced/emerging properties. So far, applying the strategy of modulation chemistry has been unable to afford high-quality functional Metal–Organic Frameworks (MOFs) nanocrystals with minimized size while exhibiting maximized defects. We report here a general sustainable strategy for the design of highly defective and ultra-small tetravalent MOFs (Zr, Hf) crystals (ca. 35% missing linker, 4–6 nm). Advanced characterizations have been performed to shed light on the main factors governing the crystallization mechanism and to identify the nature of the defects. The ultra-small nanoMOFs showed exceptional performance in peptide hydrolysis reaction, including high reactivity, selectivity, diffusion, stability, and show emerging tailorable reactivity and selectivity towards peptide bond formation simply by changing the reaction solvent. Therefore, these highly defective ultra-small M(IV)-MOFs particles open new perspectives for the development of heterogeneous MOF catalysts with dual functions.
Strong linkage between benthic oxygen uptake and bacterial tetraether lipids in deep-sea trench regions
Authors: Xiao Wenjie , Xu Yunping , Canfield Donald E. , Wenzhöfer Frank , Zhang Chuanlun , Glud Ronnie N.
Published: April 2024

Oxygen in marine sediments regulates many key biogeochemical processes, playing a crucial role in shaping Earth’s climate and benthic ecosystems. In this context, branched glycerol dialkyl glycerol tetraethers (brGDGTs), essential biomarkers in paleoenvironmental research, exhibit an as-yet-unresolved association with sediment oxygen conditions. Here, we investigated brGDGTs in sediments from three deep-sea regions (4045 to 10,100 m water depth) dominated by three respective trench systems and integrated the results with in situ oxygen microprofile data. Our results demonstrate robust correlations between diffusive oxygen uptake (DOU) obtained from microprofiles and brGDGT methylation and isomerization degrees, indicating their primary production within sediments and their strong linkage with microbial diagenetic activity. We establish a quantitative relationship between the Isomerization and Methylation index of Branched Tetraethers (IMBT) and DOU, suggesting its potential validity across deep-sea environments. Increased brGDGT methylation and isomerization likely enhance the fitness of source organisms in deep-sea habitats. Our study positions brGDGTs as a promising tool for quantifying benthic DOU in deep-sea settings, where DOU is a key metric for assessing sedimentary organic carbon degradation and microbial activity.
Physiography, foraging mobility, and the first peopling of Sahul
Authors: Salles Tristan , Joannes-Boyau Renaud , Moffat Ian , Husson Laurent , Lorcery Manon
Published: April 2024

The route and speed of migration into Sahul by Homo sapiens remain a major research question in archaeology. Here, we introduce an approach which models the impact of the physical environment on human mobility by combining time-evolving landscapes with Lévy walk foraging patterns, this latter accounting for a combination of short-distance steps and occasional longer moves that hunter-gatherers likely utilised for efficient exploration of new environments. Our results suggest a wave of dispersal radiating across Sahul following riverine corridors and coastlines. Estimated migration speeds, based on archaeological sites and predicted travelled distances, fall within previously reported range from Sahul and other regions. From our mechanistic movement simulations, we then analyse the likelihood of archaeological sites and highlight areas in Australia that hold archaeological potential. Our approach complements existing methods and provides interesting perspectives on the Pleistocene archaeology of Sahul that could be applied to other regions around the world.
In situ captured antibacterial action of membrane-incising peptide lamellae
Authors: el Battioui Kamal , Chakraborty Sohini , Wacha András , Molnár Dániel , Quemé-Peña Mayra , Szigyártó Imola Cs. , Szabó Csenge Lilla , Bodor Andrea , Horváti Kata , Gyulai Gergő , Bősze Szilvia , Mihály Judith , Jezsó Bálint , Románszki Loránd , Tóth Judit , Varga Zoltán , Mándity István , Juhász Tünde , Beke-Somfai Tamás
Published: April 2024

Developing unique mechanisms of action are essential to combat the growing issue of antimicrobial resistance. Supramolecular assemblies combining the improved biostability of non-natural compounds with the complex membrane-attacking mechanisms of natural peptides are promising alternatives to conventional antibiotics. However, for such compounds the direct visual insight on antibacterial action is still lacking. Here we employ a design strategy focusing on an inducible assembly mechanism and utilized electron microscopy (EM) to follow the formation of supramolecular structures of lysine-rich heterochiral β3-peptides, termed lamellin-2K and lamellin-3K, triggered by bacterial cell surface lipopolysaccharides. Combined molecular dynamics simulations, EM and bacterial assays confirmed that the phosphate-induced conformational change on these lamellins led to the formation of striped lamellae capable of incising the cell envelope of Gram-negative bacteria thereby exerting antibacterial activity. Our findings also provide a mechanistic link for membrane-targeting agents depicting the antibiotic mechanism derived from the in-situ formation of active supramolecules.
Dualistic insulator states in 1T-TaS2 crystals
Authors: Wang Yihao , Li Zhihao , Luo Xuan , Gao Jingjing , Han Yuyan , Jiang Jialiang , Tang Jin , Ju Huanxin , Li Tongrui , Lv Run , Cui Shengtao , Yang Yingguo , Sun Yuping , Zhu Junfa , Gao Xingyu , Lu Wenjian , Sun Zhe , Xu Hai , Xiong Yimin , Cao Liang
Published: April 2024

While the monolayer sheet is well-established as a Mott-insulator with a finite energy gap, the insulating nature of bulk 1T-TaS2 crystals remains ambiguous due to their varying dimensionalities and alterable interlayer coupling. In this study, we present a unique approach to unlock the intertwined two-dimensional Mott-insulator and three-dimensional band-insulator states in bulk 1T-TaS2 crystals by structuring a laddering stack along the out-of-plane direction. Through modulating the interlayer coupling, the insulating nature can be switched between band-insulator and Mott-insulator mechanisms. Our findings demonstrate the duality of insulating nature in 1T-TaS2 crystals. By manipulating the translational degree of freedom in layered crystals, our discovery presents a promising strategy for exploring fascinating physics, independent of their dimensionality, thereby offering a “three-dimensional” control for the era of slidetronics.
Nitrogen and Nod factor signaling determine Lotus japonicus root exudate composition and bacterial assembly
Authors: Tao Ke , Jensen Ib T. , Zhang Sha , Villa-Rodríguez Eber , Blahovska Zuzana , Salomonsen Camilla Lind , Martyn Anna , Björgvinsdóttir Þuríður Nótt , Kelly Simon , Janss Luc , Glasius Marianne , Waagepetersen Rasmus , Radutoiu Simona
Published: April 2024

Symbiosis with soil-dwelling bacteria that fix atmospheric nitrogen allows legume plants to grow in nitrogen-depleted soil. Symbiosis impacts the assembly of root microbiota, but it is unknown how the interaction between the legume host and rhizobia impacts the remaining microbiota and whether it depends on nitrogen nutrition. Here, we use plant and bacterial mutants to address the role of Nod factor signaling on Lotus japonicus root microbiota assembly. We find that Nod factors are produced by symbionts to activate Nod factor signaling in the host and that this modulates the root exudate profile and the assembly of a symbiotic root microbiota. Lotus plants with different symbiotic abilities, grown in unfertilized or nitrate-supplemented soils, display three nitrogen-dependent nutritional states: starved, symbiotic, or inorganic. We find that root and rhizosphere microbiomes associated with these states differ in composition and connectivity, demonstrating that symbiosis and inorganic nitrogen impact the legume root microbiota differently. Finally, we demonstrate that selected bacterial genera characterizing state-dependent microbiomes have a high level of accurate prediction.
Non-reciprocal band structures in an exciton–polariton Floquet optical lattice
Authors: del Valle Inclan Redondo Yago , Xu Xingran , Liew Timothy C. H. , Ostrovskaya Elena A. , Stegmaier Alexander , Thomale Ronny , Schneider Christian , Dam Siddhartha , Klembt Sebastian , Höfling Sven , Tarucha Seigo , Fraser Michael D.
Published: April 2024

Periodic temporal modulation of Hamiltonians can induce geometrical and topological phenomena in the dynamics of quantum states. Using the interference between two lasers, we demonstrate an off-resonant optical lattice for a polariton condensate with controllable potential depths and nearest-neighbour coupling strength. Temporal modulation is introduced via a gigahertz frequency detuning between pump lasers, creating a polariton ‘conveyor belt’. The breaking of time-reversal symmetry causes band structures to become non-reciprocal and acquire a universal tilt given by Planck’s constant and the frequency of modulation (hΔf). The non-reciprocal tilting is connected to the non-trivial topology of the Floquet–Bloch bands, which have a finite Chern number. Detailed characterization of the lattice potential depth and its dynamics highlights the role of high-energy carriers in the formation of optical potential landscapes for polaritons, demonstrating the possibility of modulation faster than the polariton lifetime and opening a pathway towards Floquet engineering in polariton condensates.
Fossilized anuran soft tissues reveal a new taphonomic model for the Eocene Geiseltal Konservat-Lagerstätte, Germany
Authors: Falk Daniel , Wings Oliver , Unitt Richard , Wade Jon , McNamara Maria E.
Published: April 2024

The Eocene Geiseltal Konservat-Lagerstätte (Germany) is famous for reports of three dimensionally preserved soft tissues with sub-cellular detail. The proposed mode of preservation, direct replication in silica, is not known in other fossils and has not been verified using modern approaches. Here, we investigated the taphonomy of the Geiseltal anurans using diverse microbeam imaging and chemical analytical techniques. Our analyses confirm the preservation of soft tissues in all body regions but fail to yield evidence for silicified soft tissues. Instead, the anuran soft tissues are preserved as two layers that differ in microstructure and composition. Layer 1 comprises sulfur-rich carbonaceous microbodies interpreted as melanosomes. Layer 2 comprises the mid-dermal Eberth–Katschenko layer, preserved in calcium phosphate. In addition, patches of original aragonite crystals define the former position of the endolymphatic sac. The primary modes of soft tissue preservation are therefore sulfurization of melanosomes and phosphatization of more labile soft tissues, i.e., skin. This is consistent with the taphonomy of vertebrates in many other Konservat-Lagerstätten. These findings emphasize an emerging model for pervasive preservation of vertebrate soft tissues via melanosome films, particularly in stagnation-type deposits, with phosphatization of more labile tissues where tissue biochemistry is favorable.
Posterior endpoint determination of the lumbar pedicle central axis on the anterior–posterior fluoroscopic image for pedicle screw insertion
Authors: Zhang Jun , Xu Jiawei , Xu Chenyang , Xie Youzhuan
Published: April 2024

The transpedicular procedure has been widely used in spinal surgery. The determination of the best entry point is the key to perform a successful transpedicular procedure. Various techniques have been used to determine this point, but the results are variable. This study was carried out to determine the posterior endpoint of the lumbar pedicle central axis on the standard anterior–posterior (AP) fluoroscopic images. Computer-aided design technology was used to determine the pedicle central axis and the posterior endpoint of the pedicle central axis on the posterior aspect of the vertebra. The standard AP fluoroscopic image of the lumbar vertebral models by three-dimensional printing was achieved. The endpoint projection on the AP fluoroscopic image was determined in reference to the pedicle cortex projection by the measurements of the angle and distance on the established X–Y coordinate system of the radiologic image. The projection of posterior endpoint of the lumbar pedicle central axis were found to be superior to the X-axis of the established X–Y coordinate system and was located on the pedicle cortex projection on the standard AP fluoroscopic image of the vertebra. The projection point was distributed in different sectors in the coordinate system. It was located superior to the X-axis by 18° to 26° at L1, while they were located superior to the X-axis by 12° to 14° at L2 to L5. The projections of posterior endpoints of the lumbar pedicle central axis were located in different positions on the standard AP fluoroscopic image of the vertebra. The determination method of the projection point was helpful for selecting an entry point for a transpedicular procedure with a fluoroscopic technique.
Catalytic specificity and crystal structure of cystathionine γ-lyase from Pseudomonas aeruginosa
Authors: Pedretti Marco , Fernández-Rodríguez Carmen , Conter Carolina , Oyenarte Iker , Favretto Filippo , di Matteo Adele , Dominici Paola , Petrosino Maria , Martinez-Chantar Maria Luz , Majtan Tomas , Astegno Alessandra , Martínez-Cruz Luis Alfonso
Published: April 2024

The escalating drug resistance among microorganisms underscores the urgent need for innovative therapeutic strategies and a comprehensive understanding of bacteria's defense mechanisms against oxidative stress and antibiotics. Among the recently discovered barriers, the endogenous production of hydrogen sulfide (H2S) via the reverse transsulfuration pathway, emerges as a noteworthy factor. In this study, we have explored the catalytic capabilities and crystal structure of cystathionine γ-lyase from Pseudomonas aeruginosa (PaCGL), a multidrug-opportunistic pathogen chiefly responsible for nosocomial infections. In addition to a canonical l-cystathionine hydrolysis, PaCGL efficiently catalyzes the production of H2S using l-cysteine and/or l-homocysteine as alternative substrates. Comparative analysis with the human enzyme and counterparts from other pathogens revealed distinct structural features within the primary enzyme cavities. Specifically, a distinctly folded entrance loop could potentially modulate the access of substrates and/or inhibitors to the catalytic site. Our findings offer significant insights into the structural evolution of CGL enzymes across different pathogens and provide novel opportunities for developing specific inhibitors targeting PaCGL.
Predicting 2-year neurodevelopmental outcomes in preterm infants using multimodal structural brain magnetic resonance imaging with local connectivity
Authors: Jang Yong Hun , Ham Jusung , Kasani Payam Hosseinzadeh , Kim Hyuna , Lee Joo Young , Lee Gang Yi , Han Tae Hwan , Kim Bung-Nyun , Lee Hyun Ju
Published: April 2024

The neurodevelopmental outcomes of preterm infants can be stratified based on the level of prematurity. We explored brain structural networks in extremely preterm (EP; < 28 weeks of gestation) and very-to-late (V-LP; ≥ 28 and < 37 weeks of gestation) preterm infants at term-equivalent age to predict 2-year neurodevelopmental outcomes. Using MRI and diffusion MRI on 62 EP and 131 V-LP infants, we built a multimodal feature set for volumetric and structural network analysis. We employed linear and nonlinear machine learning models to predict the Bayley Scales of Infant and Toddler Development, Third Edition (BSID-III) scores, assessing predictive accuracy and feature importance. Our findings revealed that models incorporating local connectivity features demonstrated high predictive performance for BSID-III subsets in preterm infants. Specifically, for cognitive scores in preterm (variance explained, 17%) and V-LP infants (variance explained, 17%), and for motor scores in EP infants (variance explained, 15%), models with local connectivity features outperformed others. Additionally, a model using only local connectivity features effectively predicted language scores in preterm infants (variance explained, 15%). This study underscores the value of multimodal feature sets, particularly local connectivity, in predicting neurodevelopmental outcomes, highlighting the utility of machine learning in understanding microstructural changes and their implications for early intervention.
Assessing ChatGPT 4.0’s test performance and clinical diagnostic accuracy on USMLE STEP 2 CK and clinical case reports
Authors: Shieh Allen , Tran Brandon , He Gene , Kumar Mudit , Freed Jason A. , Majety Priyanka
Published: April 2024

While there is data assessing the test performance of artificial intelligence (AI) chatbots, including the Generative Pre-trained Transformer 4.0 (GPT 4) chatbot (ChatGPT 4.0), there is scarce data on its diagnostic accuracy of clinical cases. We assessed the large language model (LLM), ChatGPT 4.0, on its ability to answer questions from the United States Medical Licensing Exam (USMLE) Step 2, as well as its ability to generate a differential diagnosis based on corresponding clinical vignettes from published case reports. A total of 109 Step 2 Clinical Knowledge (CK) practice questions were inputted into both ChatGPT 3.5 and ChatGPT 4.0, asking ChatGPT to pick the correct answer. Compared to its previous version, ChatGPT 3.5, we found improved accuracy of ChatGPT 4.0 when answering these questions, from 47.7 to 87.2% (p = 0.035) respectively. Utilizing the topics tested on Step 2 CK questions, we additionally found 63 corresponding published case report vignettes and asked ChatGPT 4.0 to come up with its top three differential diagnosis. ChatGPT 4.0 accurately created a shortlist of differential diagnoses in 74.6% of the 63 case reports (74.6%). We analyzed ChatGPT 4.0’s confidence in its diagnosis by asking it to rank its top three differentials from most to least likely. Out of the 47 correct diagnoses, 33 were the first (70.2%) on the differential diagnosis list, 11 were second (23.4%), and three were third (6.4%). Our study shows the continued iterative improvement in ChatGPT’s ability to answer standardized USMLE questions accurately and provides insights into ChatGPT’s clinical diagnostic accuracy.
Prediction and optimization method for welding quality of components in ship construction
Authors: Liu Jinfeng , Cheng Yifa , Jing Xuwen , Liu Xiaojun , Chen Yu
Published: April 2024

Welding process, as one of the crucial industrial technologies in ship construction, accounts for approximately 70% of the workload and costs account for approximately 40% of the total cost. The existing welding quality prediction methods have hypothetical premises and subjective factors, which cannot meet the dynamic control requirements of intelligent welding for processing quality. Aiming at the low efficiency of quality prediction problems poor timeliness and unpredictability of quality control in ship assembly-welding process, a data and model driven welding quality prediction method is proposed. Firstly, the influence factors of welding quality are analyzed and the correlation mechanism between process parameters and quality is determined. According to the analysis results, a stable and reliable data collection architecture is established. The elements of welding process monitoring are also determined based on the feature dimensionality reduction method. To improve the accuracy of welding quality prediction, the prediction model is constructed by fusing the adaptive simulated annealing, the particle swarm optimization, and the back propagation neural network algorithms. Finally, the effectiveness of the prediction method is verified through 74 sets of plate welding experiments, the prediction accuracy reaches over 90%.
An electrochemical aptasensor for methylamphetamine rapid detection by single-on mode based on competition with complementary DNA
Authors: Chang Wenzhuo , Zheng Zhixiang , Ma Yongjun , Du Yongling , Shi Xuezhao , Wang Chunming
Published: April 2024

A simple and rapid electrochemical sensing method with high sensitivity and specificity of aptamers was developed for the detection of methylamphetamine (MAMP). A short anti-MAMP thiolated aptamer (Apt) with a methylene blue (MB) probe at 3ʹ-end was immobilized on the surface of a gold electrode (MB-Apt-S/GE). The electrochemical signal appeared when MAMP presenting in the sample solution competed with cDNA for binding with MB-Apt-S. Under optimized conditions, the liner range of this signal-on electrochemical aptasensor for the detection of MAMP achieved from 1.0 to 10.0 nmol/L and 10.0–400 nmol/L. LOD 0.88 nmol/L were obtained. Satisfactory spiked recoveries of saliva and urine were also obtained. In this method, only 5 min were needed to incubate before the square wave voltammetry (SWV) analysis, which was much more rapid than other electrochemical sensors, leading to a bright and broad prospect for the detection of MAMP in biological sample. This method can be used for on-site rapid detection on special occasions, such as drug driving scenes, entertainment venues suspected of drug use, etc.
Population-based retrospective cohort study on community-acquired pneumonia hospitalization in children with a ventricular septal defect
Authors: Sung Myongsoon , Kim Ju Hee , Ha Eun Kyo , Shin Jeewon , Kwak Ji Hee , Jee Hye Mi , Han Man Yong
Published: April 2024

The cohort consisted of 9400 exposed children diagnosed with ventricular septal defect (VSD). The risk of community-acquired pneumonia (CAP) or asthma with VSD was assessed using the Cox proportional hazard model with an inverse probability of treatment weighting. During a mean follow-up of 6.67 years (starting from 12 months after birth), there were 2100 CAP admission cases among exposed patients (incidence rate: 33.2 per 1000 person-years) and 20,109 CAP admission cases among unexposed children (incidence rate: 29.6 per 1000 person-years), with hazard ration of 1.09 (95% CI 1.04–1.14).
Expedited re-design of multi-band passive microwave circuits using orthogonal scaling directions and gradient-based tuning
Authors: Koziel Slawomir , Pietrenko-Dabrowska Anna , Ullah Ubaid
Published: April 2024

Geometry scaling of microwave circuits is an essential but challenging task. In particular, the employment of a given passive structure in a different application area often requires re-adjustment of the operating frequencies/bands while maintaining top performance. Achieving this necessitates the utilization of numerical optimization methods. Nonetheless, if the intended frequencies are distant from the ones at the starting point, local search procedures tend to fail, whereas global search algorithms are computationally expensive. As recently demonstrated, a combination of large-scale concurrent geometry parameter scaling with intermittent local tuning allows for dependable re-design of high-frequency circuits at low CPU costs. Unfortunately, the procedure is only applicable to single-band structures due to synchronized modifications of all operating bands under scaling. This article discusses a novel procedure that leverages a similar overall concept, but allows for independent control of all center frequencies. To achieve this goal, an automated decision-making procedure is developed in which a set of orthogonal scaling directions are determined based on their effect on individual circuit bands, and using auxiliary optimization sub-problems. The scaling range is then automatically computed by solving an appropriately-defined least-square design relocation problem. The methodology introduced in the work is illustrated using two planar passive devices. In both cases, wide-range operating frequency re-design has been demonstrated and favorably compared to conventional gradient-based tuning. Furthermore, the presented procedure has been shown to be computationally efficient. It is also easy to implement and integrate with a variety of gradient-based optimization procedures of a descent type.
The use of residual analysis to improve the error rate accuracy of machine translation
Authors: Benko Ľubomír , Munkova Dasa , Munk Michal , Benkova Lucia , Hajek Petr
Published: April 2024

The aim of the study is to compare two different approaches to machine translation—statistical and neural—using automatic MT metrics of error rate and residuals. We examined four available online MT systems (statistical Google Translate, neural Google Translate, and two European commission’s MT tools—statistical mt@ec and neural eTranslation) through their products (MT outputs). We propose using residual analysis to improve the accuracy of machine translation error rate. Residuals represent a new approach to comparing the quality of statistical and neural MT outputs. The study provides new insights into evaluating machine translation quality from English and German into Slovak through automatic error rate metrics. In the category of prediction and syntactic-semantic correlativeness, statistical MT showed a significantly higher error rate than neural MT. Conversely, in the category of lexical semantics, neural MT showed a significantly higher error rate than statistical MT. The results indicate that relying solely on the reference when determining MT quality is insufficient. However, when combined with residuals, it offers a more objective view of MT quality and facilitates the comparison of statistical MT and neural MT.
Variable parameters memory-type control charts for simultaneous monitoring of the mean and variability of multivariate multiple linear regression profiles
Authors: Sabahno Hamed , Eriksson Marie
Published: April 2024

Variable parameters (VP) schemes are the most effective adaptive schemes in increasing control charts' sensitivity to detect small to moderate shift sizes. In this paper, we develop four VP adaptive memory-type control charts to monitor multivariate multiple linear regression profiles. All the proposed control charts are single-chart (single-statistic) control charts, two use a Max operator and two use an SS (squared sum) operator to create the final statistic. Moreover, two of the charts monitor the regression parameters, and the other two monitor the residuals. After developing the VP control charts, we developed a computer algorithm with which the charts' time-to-signal and run-length-based performances can be measured. Then, we perform extensive numerical analysis and simulation studies to evaluate the charts’ performance and the result shows significant improvements by using  the VP schemes. Finally, we use real data from the national quality register for stroke care in Sweden, Riksstroke, to illustrate how the proposed control charts can be implemented in practice.