bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect Of Additing Centella Asiatica L. Urban Leaves on the Moisture Content And Acceptability Of Centella Asiatica Sticks
Authors: Frenky Arif Budiman  , Suprihartini Cucuk , Wulandari Catur , Suryaningputri Rizqi Enjang
Published: August 2024

Pegagan (Centella Asiatica L. Urban) is a plant that belongs to the Umbelliferae or Apiaceae tribe. It is an herbal plant that grows on the ground, with a wide distribution area, especially in the tropics and subtropics. Currently, there is a scarcity of innovations for healthy and nutritious sticks, making it essential to explore new avenues in product development. Sticks are widelypopularamong people of all ages, from teenagers to adults. This study aims to analyze the moisture content and acceptability of gotu kola leaves sticks. The study employe a Completely Randomized Design (RAL) with one control and three treatments, namely 4%, 6% and 8%.  Organoleptic tests and water content tests were cconducted on June 15, 2022, at the Chemical Laboratory of the Karya Husada Nutrition Academy, Kediri, Indonesia. The study results revealed a significance level of  <0.05, indicating no difference in the acceptability of the color, aroma, texture and taste of pegagan leaves sticks. In the  color acceptability test, 100% of panelists most favored sticks P1 and P2., in the aroma stick acceptance test the panelists most liked sticks P1 and P2 by 100%, For stick texture acceptance, 100% of panelists favored sticks P2., In the stickiness test, the preferred sticks weres P1 by 100%. The llowestt water content, with the addition of pegagan leaves, was found in treatment 1 with a  4% addition of pegagan leaves at 2.63%. further research is needed to explore the antioxidant content and the expiration of pegagan sticks.
The Relationship between Safety Knowledge and Safety Motivation with Safety Performance among Constructions Workers of PT.X in Bandung Regency
Authors: Dwi Estu Pratiwi  , Ro'is Rachmy Rosidah , Ardyanto Wahyudiono Yustinus Denny
Published: August 2024

The prevalence of accidents in Indonesia from 2017 to 2021 has increased from 123,040 cases to 234,270 cases yearly.Individuals need to implement safety performance measures. One of the personal factors that affect safety performance is safety knowledge and safety motivation. This study aims to determine the relationship between safety knowledge and motivation with safety performance among construction workers of PT.X in Bandung Regency. This research was conducted during the PT X construction project at Bandung Regency. The research started in November 2022 and finished in January 2023. This study used a quantitative approach with observational analytics and a cross-sectional research design. The study population consists of 127 construction workers employed by PT X in Bandung Regency. The method used in this research is simple random sampling with a minimum sample size required for this study of 96 respondents. Analytics data used the chi-square test to analyze bivariate data with a significance value of 0,05. The independent variables in this study are safety knowledge and safety motivation, while the dependent variables are safety performance. The result of this research showed that there was a relationship between safety knowledge (0.00<0.05) and safety motivation (0.01<0.05) with safety performance. Increasing workers' knowledge of potential hazards, risks, and controls in the construction sector will motivate workers to behave safely in the work environment.
 
Keywords: Safety Knowledge, Safety Motivation, Safety Performance
Analysis of Factors Contributing to the Failure to Utilize the Public Health Center Information Management Systems (SIMPUS) at the Burneh Public Health Center
Authors: Daniyanti Eka Suci , Eddy Moeljono  , Fahmi Muhammad Fikri
Published: August 2024

The optimal Public Health Center Information Management Systems (SIMPUS) aims to reduce workload and improve service efficiency   in Public Health Centers. However, the patient registration process at Burneh Public Health Center is still done manually due to the inability to use, the SIMPUS application after the computer is damaged and not promptly repaired.  This manual medical record storage system adversely affects patient service duration. The objective of this study is to identify the reasons for the failure of SIMPUS usage. The method employed is descriptive with a qualitative approach. Interviews and observations serve as the research instruments. The results indicates several factors contributing to the failure of SIMPUS usage, including inadequate human resources (lack of competence officers and insufficient knowledge), unmet budgetary support for SIMPUS usage, absence of Standard Operating Procedures (SOPs) for usage, and inadequate facilities, infrastructure, and equipment to support effective SIMPUS utilization. Addressing these issues   at Burneh Public Health Center can resolve challenges associated  with SIMPUS.
Hemodialysis Patient Safety Instruments: A Systematic Review
Authors: Anwar Choirul
Published: August 2024

Hemodialysis is a technically complex procedure with many potential adverse events that can harm patients when they occur. However, hemodialysis services have not been fully implemented in standard operating procedures such as the administration process or hemodialysis programming, resulting in discomfort, adverse events, and the possibility of mild to severe patient incidents. This study aimed to review and criticize various instruments that discuss research and development of instruments to prevent adverse events in hemodialysis services. Articles from 2013 to 2023 were chosen from Scopus, Pubmed, ScienceDirect, and Web of Science. The terms used were hemodialysis safety, hemodialysis patient safety, and hemodialysis patient safety instrument based on paper, website, and technology. Overall, the review consists of 12 articles. This review discusses strategies for developing innovation, technology, or patient safety instruments and reporting to reduce adverse events and improve patient safety. The development of patient safety instruments can reduce adverse events and improve patient safety so that it can impact the aspects of improving the quality of service of a health care institution, especially hemodialysis services.
 Keywords: Dialysis patient safety instrument, Hemodialysis, Hemodialysis patient safety instrument.
Infectious Disease Health Problems at Pondok Pesantren X, Pasuruan City in 2023
Authors: Kuuni Ulfah Naila El Muna  , Dwi Handayani  , Mursyidul Ibad
Published: August 2024

Pesantren is an Islamic educational institution that provides religious education services. Pesantren usually provides dormitories for students, where many people live in one location, resulting in crowded living conditions for an extended period. If anyone who infected an infectous disease which can easily transmit to other, and moreover this can lead to an outbreak which can lead enormous losses. Therefore, we want to do an analysis situation of infectious disease that happened in Pondok Pesantren X. We managed to interview 102 santri and observed some dormitory room. Based on our analysis on the infectious disease situation, we can conclude that the diseases recorded in the clinic do not encompass all illnesses experienced by the students. Common infectious diseases among students in the Pondok Pesantren not only scabies as many people says so, there are also acute respiratory infections, gastroenteritis, typhoid, COVID-19, tuberculosis (TBC), and dengue fever (DBD). We suggest the Caregiver and administrators of the Pondok Pesantren can encourage students to avoid risky behaviors leading to infectious diseases.
أثر تمرينات خططية - مهارية في التحصيل المعرفي وأداء بعض تشكيلات الهجوم السريع الفردي والجماعي بكرة اليد للطلاب
Authors: Hassanein Sabah Baqir  , Prof. Dr. Samer Youssef Muteab
Published: August 2024

إن التعلم الحركي هو أحد العلوم الهامة والمميزة والقاعدة التي تستند عليها العملية التعليمية في مجال التربية البدنية وعلوم الرياضة وذلك لأهميته في اكتساب المتعلم كل ما هو متعلق بلعبة كرة اليد وتطويرها وإتقانها بشكل جيد ، وإنّ تعلم اداء بعض تشكيلات الهجوم السريع الفردي والجماعي يعد من أهم التشكيلات الهجومية والمصنفة ضمن مراحل الهجوم التي تعطي فرصة كبيرة للفريق المهاجم من تحقيق تسجيل الهدف بأسرع وقت وبأقل جهد ، وتتجلى مشكلة البحث في وجود ضعف في أداء بعض تشكيلات الهجوم السريع الفردي والجماعي من قبل الطلاب وكذلك صعوبة في تعلمها، لذا ارتأى الباحثان الخوض في هذه المشكلة من خلال استخدام تمرينات خططيه - مهارية وتأثيرها في تعلم اداء تشكيلات الهجوم السريع الفردي والجماعي للطلاب .
ويهدف البحث الى اعداد تمرينات خططية - مهارية لتطوير أداء بعض تشكيلات الهجوم السريع الفردي والجماعي ومعرفة اثر التمرينات الخططية - المهارية  في التحصيل المعرفي بكرة اليد لدى طلاب المرحلة الثالثة ومعرفة اثر التمرينات الخططية – المهارية في أداء بعض تشكيلات الهجوم السريع الفردي والجماعي .
Immunoglobulin prophylaxis prevents hospital admissions for fever in pediatric acute lymphoblastic leukemia: results of a multicenter randomized trial
Authors: Thus Kirsten A. , De Groot-Kruseman Hester A. , Winkler-Seinstra Pauline , Fiocco Marta , Segers Heidi , Van den Bos Cor , Van der Sluis Inge M. , Tissing Wim J.E. , Veening Margreet A. , Zwaan Christian Michel , Van Tilburg Cornelis M. , Pieters Rob , Bierings Marc
Published: August 2024

Infections lead to substantial morbidity during treatment of acute lymphoblastic leukemia (ALL) in which the adaptive immune system gets severely affected, leading to declining serum immunoglobulin levels. The aim of this trial was to investigate whether intravenous immunoglobulin (IVIG) prophylaxis in pediatric patients with ALL prevents admissions for fever.
This randomized controlled trial was a subtrial of the national Dutch multicenter ALL study. Patients aged 1-19 years with medium risk (MR) ALL were randomized into two groups receiving either IVIG prophylaxis (0.7 g/kg IVIG given every three weeks, starting day 22 after diagnosis) or well defined standard of care (control group).
Between October 2012 until March 2019, 91 (51%) patients were randomly assigned to IVIG prophylaxis and 86 (49%) to the control arm. In the IVIG prophylaxis group there were 206 admissions for fever versus 271 in the control group (p=0.011). IVIG prophylaxis was not associated with bacteremia. However, IVIG prophylaxis was associated with significantly less admissions for fever with negative blood cultures compared to the control group (N=113 versus 200, p
Repurposing CD19-directed immunotherapies for pediatric t(8;21) acute myeloid leukemia
Authors: Barneh Farnaz , Koedijk Joost B. , Wijnen Noa E. , Meulendijks Tom , Ashtiani Minoo , Dunnebach Ester , Dautzenberg Noël , Cornel Annelisa M. , Krippner-Heidenreich Anja , Klein Kim , Zwaan Michel C. , Kuball Jürgen , Nierkens Stefan , Cloos Jacqueline , Kaspers Gertjan J.L. , Heidenreich Olaf
Published: August 2024

Not available.
Hematopoietic cell transplantation for older acute myeloid leukemia patients in first complete remission: results of a randomized phase III study
Authors: Niederwieser Dietger , Hasenclever Dirk , Berdel Wolfgang , Biemond Bart J. , Al-Ali Haifa , Chalandon Yves , Van Gelder Michel , Junghanß Christian , Gahrton Gösta , Hänel Mathias , Hehlmann Rüdiger , Heinicke Thomas , Hochhaus Andreas , Iacobelli Simona , Kooy Rien van Marwijk , Kröger Nicolaus , Janssen Jeroen , Jentzsch Madlen , Breywisch Frank , Mohty Mohamad , Masouridi-Levrat Stavroula , Ossenkoppele Gert , Passweg Jacob , Pönisch Wolfram , Schetelig Johannes , Schliemann Christoph , Schwind Sebastian , Stelljes Matthias , Verdonck Leo F. , Vucinic Vladan , Löwenberg Bob , Cornelissen Jan
Published: August 2024

Given the selection of elderly patients with AML in first complete remission (CR1) the advantage of consolidation with allogeneic hematopoietic cell transplantation (HCT) over chemotherapy is still unclear.
Newly diagnosed AML patients in CR1 aged 60-75 years were registered and a donor search initiated. After one consolidation cycle, patients with a matched donor were randomized to HCT with fludarabine/lowdose total body irradiation and cyclosporine/mycophenolate mofetil immunosuppression or conventional non-HCT. Primary outcome was restricted mean leukemia-free survival (RM-LFS) up to five years.
Between 2010 and 2017, 245 patients (median age 67 years) were registered at CR1. After one consolidation, 26.9% of patients failed inclusion criteria. Of the 179 (73%) patients still on study, 75.4% had an HLA identical donor. Ten ineligible patients were excluded, and 125 randomized to HCT (n=83) or non-HCT (n=42).
The primary outcome RM-LFS up to 5 years was 24.5 months (95%CI:18.9-30.1) in the HCT and 15.6 months (95%CI:10.4-20.8) in the non-HCT arm (p=0.022) due to a decrease in cumulative relapse incidence from 91.1 (95%CI:80.7-100.0) after non-HCT to 37.8 (95%CI:27.2-48.4)% after HCT (p
Decoding Developmental Epigenetics
Authors:   , Rang Franka Jolein
Published: August 2024

The DNA is often referred to as the blueprint of the cell. However, nearly all cells in the body contain the same DNA, while displaying a vast array of phenotypes and functions. To achieve this phenotypic diversity, different types of cells activate distinct gene sets through various mechanisms, including epigenetic regulation and spatial organization of the genome. Histone post-translational modifications (PTMs) are an important form of epigenetic regulation and strongly influence chromatin density and gene expression. An example of the spatial organization of the genome is the association of chromatin with the nuclear lamina (NL), a filamentous meshwork lining the inner nuclear membrane. Regions of the DNA that are in contact with the NL are called lamina-associated domains (LADs). LADs are typically gene poor and form a gene-repressive environment. While histone PTMs and LADs have been extensively studied, a lot remains unclear about their role in regulating gene expression, especially during complex biological processes such as embryonic development. To better understand these regulatory mechanisms, it is important to measure both protein-DNA interactions and gene expression. Ideally, these measurements are taken in single cells so they can be directly related.

In my dissertation, I describe the innovations and studies that we performed during my PhD to gain more insight in epigenetic and spatial regulation. First, we developed scDam&T-seq, a technique to simultaneously measure protein-DNA interactions and gene expression in single-cells. Next, we developed EpiDamID, an extension of scDam&T-seq that enables its application to histone PTMs. We apply both these techniques to study histone PTMs and LADs during the very early stages of mouse development. During these early stages (from the moment of fertilization until implantation), the epigenome and spatial organization of the DNA are extensively rewired. The patterns inherited from the parental gametes are largely reset to enable the embryo to give rise to all cell types of the organism. In our research, we find that LADs are extremely variable in the 2-cell mouse embryo: There are large differences in which regions of the genome are LADs, both between embryos and between cells of the same embryo. We demonstrate that this variability is the result of broad domains of the histone PTM H3K27me3, which are uniquely present in the early embryo. These domains cover regions of the DNA with a strong inherent affinity for the NL, but effectively work as a repellent for NL association. These opposing forces result in the strong LAD variability and removal of H3K27me3 stabilizes LADs. These findings provide insight in the interactions between different regulatory layers of the genome and emphasize the power of single-cell technologies.
Latihan Yoga Saat Haid untuk Mengatasi Nyeri Dymenorrhea Solusi untuk Meningkatkan Kualitas Hidup Remaja Putri
Authors: Nuryaningsih  , Nurfadhilah  , Rosyati Heri , Qolbah Hayuning
Published: August 2024

Yoga saat haid merupakan kegiatan memberikan latihan gerakan yoga dalam mengurangi atau mengatasi nyeri haid. Target pengabdian ini adalah siswa Kelas VI SDN 02 dan SDN 05 Marunda. Tujuan dari program ini adalah untuk memberikan pelatihan gerakan yoga kepada siswa-siswi SDN 02 dan SDN 05 Marunda Kelas VI tentang yoga saat haid, memberikan pengetahuan tentang bagaimana melakukan gerakan yoga untuk mengatasi nyeri haid. Berdasarkan hasil penelitian sebelumnya pada bulan Maret-Agustus 2021 tentang pengaruh yoga terhadap dismenore saat menstruasi, 60% siswa SDN 05 dan SDN 02 Marunda Kelas VImengalami nyeri saat menstruasi. Hasil penelitian menemukan bahwa gerakan yoga saat menstruasi terbukti dapat mengurangi dismenore saat menstruasi. Oleh karena itu, penting untuk melakukan latihan yoga saat menstruasi untuk mengurangi intensitas dan durasi nyeri yang dapat mengganggu aktivitas, terutama aktivitas belajar. Pelaksanaan latihan gerakan yoga dilakukan secara offline dan bertahap yaitu tahap pertama untuk siswa SDN 02 Kelas VI dan tahap selanjutnya untuk siswa SDN 05 Marunda. Pelatihan yoga diberikan oleh instruktur yoga bersertifikat dan dilaksanakan dengan menerapkan protokol kesehatan COVID-19 secara ketat. Untuk mengevaluasi efektivitas kegiatan, siswa Kelas VI SD diminta mengulang gerakan yoga saat haid, dan setelah itu siswa Kelas VI SD diberikan video yoga agar dapat mengulang gerakan yoga di rumah dan memperkuat daya ingat terhadap gerakan yoga tersebut. gerakan sehingga mereka dapat melakukannya dengan baik dan benar. Pemberian pelatihan yoga diharapkan dapat mengurangi atau mengatasi rasa sakit saat mengalami menstruasi, sehingga kualitas hidup remaja putri akan meningkat.
Becoming White in the American Enlightenment
Authors: Bow Charles Bradford
Published: August 2024

This article critically examines the different ways in which American Enlightenment thinkers confronted the future of being white in the early republic as political economists, natural historians, physicians, and anatomists of the mind. Just as philosophes experimented on the nature of blackness in Bordeaux's Royal Academy of Sciences, the American Philosophical Society supported novel approaches to the racial classification of whiteness. An appeal to Scottish Enlightenment racial theories connected the diverging thought of Benjamin Franklin, Thomas Jefferson, Samuel Stanhope Smith, and Benjamin Rush as prominent members of this society between 1743 and 1826. In doing so, Franklin industrialised the political economy of a ‘purely white’ nation. Jefferson naturalised the language of whiteness. Smith universalised the intellectual and moral cultivation of whiteness. And Rush medicalised an architype of a healthy mind and body as white. This article argues that Scottish philosophy informed radically different ideas about how to become white in the early American republic.
Just as Then, So Now: The Slave as Constitutive of the Pauline Symbolic Order
Authors: Thwaites Julia
Published: August 2024

The letters of the apostle Paul have enjoyed an unusually exalted status in both secular philosophical and theological projects of universalism of recent decades, which have argued that Paul's decisive move in the establishment of early Christianity is his negation of the signifying force and particularism of the flesh in favour of the universalism of the community of spirit. No passage illustrates this aim more plainly than Galatians 3:28, in which Paul states ‘There is no longer Jew or Greek; there is no longer slave or free; there is no longer male and female, for all of you are one in Christ Jesus.’ Yet, while Paul appears to annihilate the fleshly categories that had hitherto served as the basis of inclusion or exclusion, he turns time and again to the figure of the slave as a fixed point of signification that is seemingly essential to the success of the new symbolic order. Drawing on the work of Michel de Certeau, Elaine Scarry and Page duBois, it becomes clear that it is through technologies of slavery – that is, torture, tattooing, and death – that the figure of the slave functions as an instrument through which to produce an authoritative and totalising ontology out of the new symbolic order by indicating that beyond its limit there lies only annihilating pain and death. The slave thus functions as both point de capiton – Lacan's term for the fixed point that stabilises the symbolic order and renders it legible – and as an ‘oath,’ a mechanism for making language coterminous with truth through threat of violence, as explicated by Giorgio Agamben. Through his maintenance of the figure of the slave, Paul thus forecloses on the possibility of a true universal, finding instead a symbolic order that is made total through force.
Fugitive Freedom in Spinoza
Authors: Sharp Hasana
Published: August 2024

Drawing on Black radical thought, some political theorists have elaborated a notion of ‘fugitive freedom’ that challenges us to understand freedom beyond the canonical concepts of ‘positive’ and ‘negative’ liberty. The idea of fugitive freedom concerns the vast liminal space between being enslaved and enjoying complete political (or ethical) liberty. Whereas for traditional political theory, there are two ‘conditions’ or ‘statuses’ assigned to subjects (‘free’ or ‘slave’), reflection on slave narratives and the history of maroon communities points to freedom expressed in escape, flight, and movement away from domination. Fugitive freedom is enabled and sustained through quotidian, clandestine practices of communication, concealment, care, and refuge. Inspired by these exhortations to reimagine the forms that freedom can take, this paper finds in Spinoza a notion of fugitivity, understood as a counter-power, animated by the desire to escape domination, generate solidarity, and forge mental community.
Social Death as a Kind of Deconstruction: The Figure of the Slave Under Erasure
Authors: Drabinski John E.
Published: August 2024

This essay explores the theoretical resources generated by a critical reading of deconstruction with the theory of social death. Both offer accounts of life and death in which life is dependent upon a certain form of death. What to make of that dependency? And what would it mean to exceed that dependency, in the mode of deconstructive supplementarity? I work these questions through three examples: John Locke's account of property and propriety, Immanuel Kant's notion of the categorical imperative, and G.W.F. Hegel's description of world history. In each, I identify how the argument turns on a figure of the slave – an unremunerated rhetorical and logical element that, when re-read under erasure, fails to buttress the system in which it is deployed and instead destabilizes the argument and its historical-cultural politics.
Disproving the Euler Product equal to the Riemann Zeta Function
Authors: Annamalai Chinnaraji
Published: August 2024

The author of this article has already disproved the Euler product equal to the Riemann zeta function that the earlier mathematicians proved. This article presents the main reason why the Euler product is not equal to the Riemann zeta function.
Lesiones deportivas en el baloncesto y su prevención: Revisión sistemática
Authors: Adámez Gordo Rafael , Mancha Triguero David , Campos Redondo Amalia , Martínez Sánchez Almudena , García Rubio Javier
Published: August 2024

Las lesiones deportivas son una problemática que afecta a todos los practicantes a cualquier actividad física sin importar la edad, sexo, nivel deportivo o experiencia. Una de las facetas que tiene el entrenamiento sobre los deportistas es reducir la probabilidad de lesión mediante el entrenamiento físico o preventivo. Objetivo: El objetivo de esta investigación fue realizar dos revisiones bibliográficas: una de ellas centrada en conocer la etiología de las lesiones de los practicantes de baloncesto, y la segunda sobre los métodos más empleados para la prevención de las lesiones. Método: Para ambas revisiones se realizó una búsqueda bibliográfica en la WOS de los estudios publicados en los últimos 10 años, obteniendo 57 artículos para la primera revisión y 15 estudios para la segunda. Resultados: Los resultados han mostrado que las lesiones más comunes se producen en la rodilla y tobillo, que los jugadores masculinos se lesionan más que los femeninos, siendo afectada con mayor frecuencia los tejidos blandos, sin contactar con otro jugador y durante la competición. Entre las técnicas preventivas activas más empleadas se encuentra el entrenamiento propioceptivo y neuromuscular, mientras que en las técnicas pasivas destacan el descanso y el empleo de tobilleras. Conclusiones: Por todo ello, se concluye que son necesarios más estudios que reúnan la etiología de las lesiones de los practicantes de baloncesto, puesto que los resultados son heterogéneos.
CAMPUR KODE DALAM PERCAKAPAN GRUP KERJA WHATSAPP KARYAWAN
Authors: Cita Cita Dwi Yuniasih , Rani Gustiasari Dewi
Published: August 2024

This research was conducted to provide information to readers regarding the form of code mixing, as well as how the code interference event can occur. This is also used as a learning for writers, in training analytical skills, and is expected to be used as something that can inform readers. This study also contains what factors cause code interference in Whatsapp workgroup conversations in PT employees. Alita Praya Mitra. The research methods used are qualitative methods, identification and recording techniques, as well as classifying and explaining data to analyze conversation data. The results of the analysis that have been examined, there is a mixture of codes when discussing between employees and employees, and between employees and superiors during working hours. The factor that influences the occurrence of code interference is the number of employees who carry out work from home / Work From Home (WFH) and the use of code mix can give a formal impression as a form of employee professionalism in their work.
ANALISIS TINDAK TUTUR EKSPRESIF PADA DAFTAR PUTAR VIDEO KULIAH NEGOSIASI DALAM CHANNEL YOUTUBE KULIAH KEHIDUPAN
Authors: Utomo Asep Purwo Yudi , Azki Akhmad Mafaza  , Novana Ika Puspita  , Fadhila Ramadhani  , Diyas Bagus Firmansyah  , Riyadi Widhiyanto  , Rossi Galih Kesuma
Published: August 2024

Pragmatics is a field of linguistic study that relies on context as the main means of understanding an intention. One of its areas of study is expressive speech acts, which are used to express or convey someone's emotional and feeling states. The aim of this research is to describe and analyze various types of expressive speech acts found in the "Negotiation Lecture" videos on the "Kuliah Kehidupan" YouTube channel. This research employs a qualitative descriptive methodological approach and a theoretical pragmatic analysis approach. The data sources used are derived from conversations or expressive utterances found in the negotiation lecture videos on the "Kuliah Kehidupan" YouTube channel. Data collection in this article uses the observation and note-taking technique. The data analysis technique employs distributional and matching techniques. The results of this research found ten types of speech acts in the "Negotiation Lecture" videos on the "Kuliah Kehidupan" YouTube channel, namely expressive speech acts of criticizing, hoping, complaining, blaming, praising, apologizing, thanking, expressing anger, congratulating, and expressing fear. This research is beneficial for providing new insights into the classification of expressive speech acts found in the videos. It is expected that this research can serve as a reference for students, lecturers, or learners, and as material for further research in the context of learning in pragmatic studies, especially regarding the types of expressive speech acts.
ANALISIS KESALAHAN BERBAHASA PADA SPANDUK DAN PAPAN REKLAME SEBAGAI MEDIA PROMOSI
Authors: Herwani Suci , Inti Khatun Nafia
Published: August 2024

Banners and billboards are promotional media that are widely used by people to promote their businesses. It is not uncommon for the words or sentences used to influence the reader. However, many banner and billboard makers ignore the rules of writing. This research aims to analyze language errors on banners and billboards in Kudus Regency. The research method used is descriptive qualitative. The research data is the use of Indonesian on banners and billboards. The data analysis technique uses the intralingual matching method. The research results found several language errors on banners and billboards including standard word errors, writing foreign terms, writing abbreviations, and writing numbers. By knowing the language errors that arise, it can help writers to be more careful in their writing and must follow the rules or writing rules in the guidelines.
Characterization of forest fragments occupied by the critically endangered and endemic San Martín titi monkey (Plecturocebus oenanthe)
Authors: Rubio Vargas Carina Linda , Cerón Cancharis Zoila Lasmit , Heymann Eckhard W.
Published: August 2024

This study describes the characteristics of forest fragments occupied by a Critically Endangered endemic Peruvian primate, the San Martín titi monkey, Plecturocebus oenanthe (Pitheciidae; Platyrrhini). We selected 45 fragments; 20 had already been surveyed in 2015 by the Proyecto Mono Tocón (six of these had been further split, resulting in 27 fragments); an additional 18 fragments were randomly selected from satellite images. We surveyed these fragments for the presence of P. oenanthe and determined characteristics of the fragments (size, shape, tree density, canopy height) and of the landscape (distance to nearest fragment and road). We also examined changes in the number of fragments and in forest cover between 2015 and 2019. We encountered P. oenanthe in all surveyed fragments except for the smallest one (0.2 ha). Our findings suggest that P. oenanthe can persist in fragments with a wide range of characteristics, particularly with regard to size and tree density. Unless fragmentation continues and overall forest cover in the area diminishes further, the species may be able to persist even in a fragmented landscape, provided that the matrix allows for movements between fragments. However, persistence might not be long-term if groups are not reproductive, populations become too small, and reduced gene flow results in inbreeding.
Associations between first-trimester screening biomarkers and maternal characteristics with gestational diabetes mellitus in Chinese women
Authors: Lu Yu-Ting , Chen Chie-Pein , Sun Fang-Ju , Chen Yi-Yung , Wang Liang-Kai , Chen Chen-Yu
Published: August 2024

Background

Gestational diabetes mellitus (GDM) can result in adverse maternal and neonatal outcomes. Predicting those at high risk of GDM and early interventions can reduce the development of GDM. The aim of this study was to examine the associations between first-trimester prenatal screening biomarkers and maternal characteristics in relation to GDM in Chinese women.

Methods

We conducted a retrospective cohort study of singleton pregnant women who received first-trimester aneuploidy and preeclampsia screening between January 2019 and May 2021. First-trimester prenatal screening biomarkers, including pregnancy-associated plasma protein A (PAPP-A), free beta-human chorionic gonadotropin, and placental growth factor (PLGF), along with maternal characteristics, were collected for analysis in relation to GDM. Receiver operating characteristic (ROC) curve and logistic regression analyses were used to evaluate variables associated with GDM.

Results

Of the 1452 pregnant women enrolled, 96 developed GDM. PAPP-A (5.01 vs. 5.73 IU/L, P < 0.001) and PLGF (39.88 vs. 41.81 pg/mL, P = 0.044) were significantly lower in the GDM group than in the non-GDM group. The area under the ROC curve of combined maternal characteristics and biomarkers was 0.73 (95% confidence interval [CI] 0.68–0.79, P < 0.001). The formula for predicting GDM was as follows: P = 1/[1 + exp (-8.148 + 0.057 x age + 0.011 x pregestational body mass index + 1.752 x previous GDM history + 0.95 x previous preeclampsia history + 0.756 x family history of diabetes + 0.025 x chronic hypertension + 0.036 x mean arterial pressure - 0.09 x PAPP-A - 0.001 x PLGF)]. Logistic regression analysis revealed that higher pregestational body mass index (adjusted odds ratio [aOR] 1.03, 95% CI 1.01 - 1.06, P = 0.012), previous GDM history (aOR 9.97, 95% CI 3.92 - 25.37, P < 0.001), family history of diabetes (aOR 2.36, 95% CI 1.39 - 4.02, P = 0.001), higher mean arterial pressure (aOR 1.17, 95% CI 1.07 - 1.27, P < 0.001), and lower PAPP-A level (aOR 0.91, 95% CI 0.83 - 1.00, P = 0.040) were independently associated with the development of GDM. The Hosmer-Lemeshow test demonstrated that the model exhibited an excellent discrimination ability (chi-square = 3.089, df = 8, P = 0.929).

Conclusion

Downregulation of first-trimester PAPP-A and PLGF was associated with the development of GDM. Combining first-trimester biomarkers with maternal characteristics could be valuable for predicting the risk of GDM.
Causal effects of autoimmune diseases on thyroid cancer: a two-sample Mendelian randomization study
Authors: Peng Wenfang , Xu Bojin , Zhou Haiping , Du Juan , Ge Xiaoxu , Huang Shan
Published: August 2024

Background

Although numerous studies had revealed associations between autoimmune diseases (AIDs) and thyroid cancer (TC), the potential causal associations between the two remain poorly defined.

Methods

Using five approaches, two-sample Mendelian randomization (MR) analyses were carried out to determine the causal effects of 12 major AIDs on risk of TC. The sensitivity analyses were conducted to verify the reliability of the analysis. The reverse MR analysis was performed to evaluate the possibility of reverse causation.

Results

The results showed a significant causal association of systemic lupus erythematosus (SLE) and primary biliary cirrhosis (PBC) on the risk of TC. Genetically predicted PBC elevated the risk of TC (OR = 1.46, 95% CI = 1.06-2.02, p = 0.021). The risk of TC was also increased by genetically predicted SLE (OR = 6.52, 95% CI = 1.38-30.84, p = 0.018) with heterogeneity. After outlier-corrected analyses, the results still suggested that genetically predicted SLE increased the risk of TC (p = 0.019). No evidence of a causal relationship between the remaining 10 AIDs and TC was observed. No reverse causal effects of TC on AIDs were found in reverse MR analysis.

Conclusion

These findings support a significant causal association of SLE/PBC on the increased risk of TC, indicating that patients with SLE/PBC should be under a close monitoring of TC.
Proteomics profiling reveals lipid metabolism abnormalities during oogenesis in unexplained recurrent pregnancy loss
Authors: Liu Kun , Xu Xiaojuan , Sun Liang , Li Hongxing , Jin Yi , Ma Xiaoling , Shen Bairong , Martin Cesar
Published: August 2024

Background

Unexplained recurrent pregnancy loss (URPL) is a clinical dilemma in reproductive fields. Its diagnosis is mainly exclusionary after extensive clinical examination, and some of the patients may still face the risk of miscarriage.

Methods

We analyzed follicular fluid (FF) from in vitro fertilization (IVF) in eight patients with URPL without endocrine abnormalities or verifiable causes of abortion and eight secondary infertility controls with no history of pregnancy loss who had experienced at least one normal pregnancy and delivery by direct data-independent acquisition (dDIA) quantitative proteomics to identify differentially expressed proteins (DEPs). In this study, bioinformatics analysis was performed using online software including g:profiler, String, and ToppGene. Cytoscape was used to construct the protein–protein interaction (PPI) network, and ELISA was used for validation.

Results

Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the DEPs are involved in the biological processes (BP) of complement and coagulation cascades. Apolipoproteins (APOs) are key proteins in the PPI network. ELISA confirmed that APOB was low-expressed in both the FF and peripheral blood of URPL patients.

Conclusion

Dysregulation of the immune network intersecting coagulation and inflammatory response is an essential feature of URPL, and this disequilibrium exists as early as the oogenesis stage. Therefore, earlier intervention is necessary to prevent the development of URPL. Moreover, aberrant lipoprotein regulation appears to be a key factor contributing to URPL. The mechanism by which these factors are involved in the complement and coagulation cascade pathways remains to be further investigated, which also provides new candidate targets for URPL treatment.
Recent advances in the clinical development of antifungal vaccines: a narrative review
Authors: Alapan Das , Bisweswar Ojha , Prasenjit Sarkar , Prasanjit Das , Arkapal Bandyopadhyay
Published: August 2024

Vaccine development tools for fungal infections are undergoing transformation where newer technologies like nanotechnology and bioinformatics are used to create new and improved vaccine candidates. Immunocompromised individuals and those with multiple chronic conditions are especially vulnerable to invasive fungal infections. These patients are at increased risk of developing widespread infections and experiencing poor health outcomes. Current management of fungal infections is associated with diagnostic challenges, side effects, and resistance. Vaccination is an effective strategy to prevent infections and boost immunity. Despite the significant burden of fungal disease, there are currently no licensed fungal vaccines available. This review is focused on various vaccine development strategies, including whole-cell, subunit, and nucleic acid-based vaccines. Various challenges like safety concerns, weak and nonspecific immune response, ideal adjuvants, and the need for improved drug delivery systems are also highlighted in this review. Sustained antigenic response, addressing host immune response variability, and eliciting persistent predictable immune response are crucial for vaccine development. Standardized protocols and robust preclinical studies are essential for the clinical development of potential vaccine candidates. Exploring novel targets using advanced technologies like bioinformatics, nanotechnology, and reverse vaccinology are being rapidly explored.
Anti-HIV activity in traditional Chinese medicine: clinical implications of monomeric herbal remedies and compound decoctions
Authors: Zhang Nannan , Wang Mengyuan , Gao Ling , Zhang Congying , Tang Xiaoguang , Liu Xianjun , Bai Chunying
Published: August 2024

With the global spread of human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS), the pursuit of potent treatments has ascended as a paramount concern in global healthcare. Traditional Chinese medicine (TCM) has been used for thousands of years in China and other East Asian countries and it offers remedies for an extensive array of ailments, including HIV and AIDS. This review focuses on the clinical significance of single herbs and composite tonics in TCM with antiviral activity against HIV. Initially, the anti-HIV activity of single herbs was analyzed in detail. Many herbs have been shown to have significant anti-HIV activity. The active ingredients of these herbs exhibit their anti-HIV effects through various mechanisms, such as inhibiting viral replication, preventing viral binding to host cells, and interfering with the viral lifecycle. Furthermore, we delved into the clinical significance of HIV-associated formulations provided as a result of Chinese compound prescription. These combinations of herbal ingredients are designed to amplify therapeutic efficacy and minimize adverse effects. Clinical trials have demonstrated the therapeutic benefits of these prescriptions for individuals infected with HIV. The intricate composition of these prescriptions potentially augments their anti-HIV activity through synergistic effects. Additionally, this review underscores the clinical importance of TCM in the context of HIV treatment. While numerous herbs and prescriptions exhibit anti-HIV activity, their safety and efficacy in clinical applications warrant further investigation. When combined with contemporary antiretroviral drugs, TCM may serve as an adjunctive therapy, assisting in reducing side effects, and enhancing patients' quality of life. To optimally harness these natural resources, further exploration is imperative to ascertain their efficacy, safety, and optimal utilization, thereby offering a broader spectrum of therapeutic options for HIV-afflicted individuals.
Epidemic trend, genetic characteristics, and transmission networks of HIV-1 among treatment-naive men who have sex with men in Hebei province, China
Authors: Lu Xinli , Wang Yingying , Ma Lin , Liu Meng , Li Yan , An Ning , Zhang Xinyu , Tang Xiangyun , Li Qi
Published: August 2024

Introduction

Homosexual transmission has contributed greatly to the current HIV-1 epidemic in Hebei province, China. Dolutegravir (DTG) will be conditionally used as a component of free antiretroviral therapy (ART) according to manual for national free anti-AIDS treatment drugs (2023 edition) issued by China in June 2023. However, current genetic characteristics and pretreatment drug resistance (PDR) to proteinase inhibitors (PIs), reverse transcriptase inhibitors (RTs) and integrase strand transfer inhibitors (INSTIs) of HIV-1 in this population have remained unclear.

Methods

Serial consecutive cross-sectional analyses for HIV- 1 infection trend, genetic characteristics, PDR and molecular transmission networks were conducted from 2018 to 2022. All of participants were HIV-1- infected MSM newly diagnosed at the HIV surveillance points (HSPs) in Hebei, China. Evidence of PDR was confirmed using the world health organization (WHO) list for surveillance of drug resistance mutations.

Results

In this study, a total of 14 HIV-1 subtypes were circulating in the HSPs of Hebei province, China. CRF01_ AE (51.9%, 350/675), CRF07_BC (30.4%, 205/675), B (6.2%, 42/675) and URFs (5.8%, 39/675) were the four most predominant subtypes among MSM. And, CRF07_BC (r > 0) and URFs (r > 0) indicated an increasing trend, respectively; however, CRF01_AE (r < 0) showed a decline trend. The overall prevalence of HIV-1 PDR showed a substantial increase from 6.3% in 2018 to 7.9% in 2022. The prevalence of NNRTI-PDR was the highest (5.8%, 39/675), followed by INSTIs (2.4%, 16/675), NRTIs (0.6%, 4/675) and PIs (0.3%, 2/675). Furthermore, extensive HIV-1 strains bearing PDR were circulating in the MSM population via molecular transmission networks for major HIV-1 subtypes, especially CRF01_AE and CRF07_BC.

Discussion

Our findings reflect that HIV-1 epidemic in the MSM population is complex and severe in Hebei, China. Therefore, it is urgent for us to implement more effective intervention measures to limit the further dissemination of HIV-1, especially the spread of HIV-1 INSTI-PDR strains.
Hyperforin, the major metabolite of St. John’s wort, exhibits pan-coronavirus antiviral activity
Authors: Raczkiewicz Imelda , Rivière Céline , Bouquet Peggy , Desmarets Lowiese , Tarricone Audrey , Camuzet Charline , François Nathan , Lefèvre Gabriel , Silva Angulo Fabiola , Robil Cyril , Trottein François , Sahpaz Sevser , Dubuisson Jean , Belouzard Sandrine , Goffard Anne , Séron Karin
Published: August 2024

Introduction

The COVID-19 pandemic caused by the SARS-CoV-2 virus has underscored the urgent necessity for the development of antiviral compounds that can effectively target coronaviruses. In this study, we present the first evidence of the antiviral efficacy of hyperforin, a major metabolite of St. John’s wort, for which safety and bioavailability in humans have already been established.

Methods

Antiviral assays were conducted in cell culture with four human coronaviruses: three of high virulence, SARS-CoV-2, SARS-CoV, and MERS-CoV, and one causing mild symptoms, HCoV-229E. The antiviral activity was also evaluated in human primary airway epithelial cells. To ascertain the viral step inhibited by hyperforin, time-of-addition assays were conducted. Subsequently, a combination assay of hyperforin with remdesivir was performed.

Results

The results demonstrated that hyperforin exhibited notable antiviral activity against the four tested human coronaviruses, with IC50 values spanning from 0.24 to 2.55 µM. Kinetic studies indicated that the observed activity occur at a post-entry step, potentially during replication. The antiviral efficacy of hyperforin was additionally corroborated in human primary airway epithelial cells. The results demonstrated a reduction in both intracellular and extracellular SARS-CoV-2 viral RNA, confirming that hyperforin targeted the replication step. Finally, an additive antiviral effect on SARS-CoV-2 was observed when hyperforin was combined with remdesivir.

Discussion

In conclusion, hyperforin has been identified as a novel pan-coronavirus inhibitor with activity in human primary airway epithelial cells, a preclinical model for coronaviruses. These findings collectively suggest that hyperforin has potential as a candidate antiviral agent against current and future human coronaviruses.
O-GlcNAc impacts mitophagy via the PINK1-dependent pathway
Authors: Alghusen Ibtihal M. , Carman Marisa S. , Wilkins Heather M. , Strope Taylor A. , Gimore Caleb , Fedosyuk Halyna , Shawa Jad , Ephrame Sophiya John , Denson Aspin R. , Wang Xiaowan , Swerdlow Russell H. , Slawson Chad
Published: August 2024

Background

The accumulation of dysfunctional mitochondria is an early feature of Alzheimer’s disease (AD). The impaired turnover of damaged mitochondria increases reactive oxygen species production and lowers ATP generation, leading to cellular toxicity and neurodegeneration. Interestingly, AD exhibits a disruption in the global post-translational modification β-N-acetylglucosamine (O-GlcNAc). O-GlcNAc is a ubiquitous single sugar modification found in the nuclear, cytoplasmic, and mitochondrial proteins. Cells maintain a homeostatic level of O-GlcNAc by cycling the addition and removal of the sugar by O-GlcNAc transferase (OGT) or O-GlcNAcase (OGA), respectively.

Methods

We used patient-derived induced pluripotent stem cells, a transgenic mouse model of AD, SH-SY5Y neuroblastoma cell lines to examine the effect of sustained O-GlcNAcase inhibition by Thiamet-G (TMG) or OGT deficiency on mitophagy using biochemical analyses.

Results

Here, we established an essential role for O-GlcNAc in regulating mitophagy (mitochondria-selective autophagy). Stimulating mitophagy using urolithin A (UA) decreases cellular O-GlcNAc and elevates mitochondrial O-GlcNAc. Sustained elevation in O-GlcNAcylation via pharmacologically inhibiting OGA using Thiamet-G (TMG) increases the mitochondrial level of mitophagy protein PTEN-induced kinase 1 (PINK1) and autophagy-related protein light chain 3 (LC3). Moreover, we detected O-GlcNAc on PINK1 and TMG increases its O-GlcNAcylation level. Conversely, decreasing cellular O-GlcNAcylation by knocking down OGT decreases both PINK1 protein expression and LC3 protein expression. Mitochondria isolated from CAMKII-OGT-KO mice also had decreased PINK1 and LC3. Moreover, human brain organoids treated with TMG showed significant elevation in LC3 compared to control. However, TMG-treated AD organoids showed no changes in LC3 expression.

Conclusion

Collectively, these data demonstrate that O-GlcNAc plays a crucial role in the activation and progression of mitophagy, and this activation is disrupted in AD.