bluuebunny/crossref_metadata_2025 huggingface dataset:
Unmanned Ground Vehicle for Identifying Trees Infested with Bark Beetles
Authors: Flores Jonathan , Salazar Sergio , González-Hernández Iván , Rosales-Luengas Yukio , Lozano Rogelio
Published: December 2024

This research presents an unmanned ground vehicle for identifying infested trees by bark beetles in mountain forests. The ground vehicle uses sensors for autonomous navigation and obstacle avoidance. The identification of infested trees is carried out by classifying the resin stains on the bark of unhealthy trees with a computer vision algorithm. This approach proposes tracking bark beetle spread in forest trees with image data of the infested trees considering resin sprouts as early indicators of the infestation in contrast to aerial monitoring, which only detects trees in advanced stages. Terrain autonomous vehicle direction is controlled by changing the velocities of left- and right-side wheels. A rotating LiDAR sensor is used to detect trees and avoid objects. The dynamic model of the vehicle is presented, and a control algorithm is proposed for path-following. Moreover, the stability of the system is proven using a Lyapunov function. In order to demonstrate the performance of the control and classification algorithms, experimental results from an outdoor forest environment are presented.
Wrist Exoskeleton Actuated by a 2-RRU-U Parallel Manipulator
Authors: Crăciun Narcis-Graţian , Tulcan Elida-Gabriela , Oarcea Alexandru , Ciapa Cristian , Lovasz Erwin-Christian
Published: December 2024

This article has as the main subject the exoskeleton devices used in rehabilitation applications of the wrist joint for patients who are in the recovery period after suffering a stroke. Exoskeleton devices have some advantages compared to classic therapies, such as the possibility of performing rehabilitation exercises from the comfort of your own home. Therefore, in this article, a short state of the art with the main devices developed at the present time was carried out. By developing a structural analysis, it was shown that the parallel manipulator structures used for the development of the exoskeleton have 2 DoF, and they can contain RRU, RTU, or TRU connection legs. Next, the kinematic analysis of the structure with RRU connection legs is presented, from which the transmission function for the four-bar linkages was extracted. The study continues with the optimization of the synthesis in order to achieve a higher value for the minimum input transmission angle and a lower value for the total size area. Using the optimized results, a 3D prototype and the performance analysis were developed. The main conclusion of this article is that the presented 2-RRU-U parallel manipulator can be a suitable proposal for a rehabilitation exoskeleton-type device due to the optimization of the total size area and the higher value of the minimum input transmission angle.
Generalisation of the Signed Distance
Authors: Berkachy Rédina , Donzé Laurent
Published: December 2024

This paper presents a comprehensive study of the signed distance metric for fuzzy numbers. Due to the property of directionality, this measure has been widely used. However, it has a main drawback in handling asymmetry and irregular shapes in fuzzy numbers. To overcome this rather bad feature, we introduce two new distances, the balanced signed distance (BSGD) and the generalised signed distance (GSGD), seen as generalisations of the classical signed distance. The developed distances successfully and effectively take into account the shape, the asymmetry and the overlap of fuzzy numbers. The GSGD is additionally directional, while the BSGD satisfies the requirements for being a metric of fuzzy quantities. Analytical simplifications of both distances in the case of often-used particular types of fuzzy numbers are provided to simplify the computation process, making them as simple as the classical signed distance but more realistic and precise. We empirically analyse the sensitivity of these distances. Considering several scenarios of fuzzy numbers, we also numerically compare these distances against established metrics, highlighting the advantages of the BSGD and the GSGD in capturing the shape properties of fuzzy numbers. One main finding of this research is that the defended distances capture with great precision the distance between fuzzy numbers; additionally, they are theoretically appealing and are computationally easy for traditional fuzzy numbers such as triangular, trapezoidal, Gaussian, etc., making these metrics promising.
Simulation of a Reverse Electrodialysis–Absorption Refrigeration Integration System for the Efficient Recovery of Low-Grade Waste Heat
Authors: Wu Xi , Yan Linjing , Zhu Xiaojing , Liu Mingjun
Published: December 2024

The absorption refrigeration system (ARS) stands as a remarkable device that is capable of efficiently harnessing low-grade thermal energy and converting it into cooling capacity. The reverse electrodialysis (RED) system harvests the salinity gradient energy embedded in two solutions of different concentrations into electricity. An innovative RED–ARS integration system is proposed that outputs cooling capacity and electric energy, driven by waste heat. In this study, a comprehensive mathematical simulation model of a RED–ARS integration system was established, and an aqueous lithium bromide solution was selected as the working solution. Based on this model, the authors simulated and analyzed the impact of various factors on system performance, including the heat source temperature (90 °C to 130 °C), concentrated solution concentration (3 mol∙L⁻1 to 9 mol∙L⁻1), dilute solution concentration (0.002 mol∙L⁻1 to 0.5 mol∙L⁻1), condensing temperature of the dilute solution (50 °C to 70 °C), solution temperature (30 °C to 60 °C) and flow rate (0.4 cm∙s⁻1 to 1.3 cm∙s⁻1) in the RED stacks, as well as the number of RED stacks. The findings revealed the maximum output power of 934 W, a coefficient of performance (COP) of 0.75, and overall energy efficiency of 33%.
X-Ray Performance of SiC NPN Radiation Detector
Authors: Wang Jing , Zhou Leidang , Chen Liang , Zhang Silong , Wang Fangbao , Fan Tingting , Chen Zhuo , Bai Song , Ouyang Xiaoping
Published: December 2024

In this paper, a silicon carbide (SiC) phototransistor based on an open-base structure was fabricated and used as a radiation detector. In contrast to the exposed and thin sensitive region of traditional photo detectors, the sensitive region of the radiation detector was much thicker (30 μm), ensuring the high energy deposition of radiation particles. The response properties of the fabricated SiC npn radiation detector were characterized by high-energy X-ray illumination with a maximum X-ray photon energy of 30 keV. The SiC npn detector featured stable and clear response to the X-ray within 0.0766 Gy∙s−1 to 0.766 Gy∙s−1 below 300 V. Due to to the low leakage current of less than 1 nA and the fully depleted sensitive region, the bipolar-transistor-modeled SiC npn detector exhibited a clear common-emitter current gain of 5.85 at 200 V (under 0.383 Gy∙s−1), where the gain increased with bias voltage due to the Early effect and reached 7.55 at 300 V. In addition, the transient response of the SiC npn detector revealed a longer delay time than the SiC diode of the same size, which was associated with the larger effective capacitance of the npn structure. The npn detector with internal gain showed great potential in radiation detection.
Plasma-Treated Water Retards Pellicle-like Biofilm Formation of Bacillus subtilis
Authors: Weihe Thomas , Wallis Jan , Meister Mareike , Ehlbeck Jörg , Schnabel Uta
Published: December 2024

Plasma-treated water (PTW) recently entered science as a sanitizing agent, which possess the capability for on-demand production. It offers interesting possibilities for sustainable and resource-saving applications in healthcare and food production. The present study monitors the impact of PTW on suspended cells before the biofilm formation of the putrefactive bacterium B. subtilis. Light and electron microscope imaging captures the maturing of growing biofilms within the first 24 h. Microbiological assays (proliferation, LIVE/DEAD, and XTT), which mirror the proliferation of the bacterium, the metabolic activity, and the integrity of the cell membrane, underpinning the metabolic response of still-suspended cells. B. subtilis cells without any treatment build up a resistive biofilm within the 24 h. Cells that remain in the supernatant predominantly appear as monomers or dimers. Treated B. subtilis cells have hampered biofilm formation and were not able to build up a confluent growing biofilm within the first 24 h. Moreover, the microscopic observation of PTW-treated suspension showed cellular aggregates with an unusually high connectivity of the individual cells. The findings suggest this cellular reaction as a counter measure against the adverse impact of PTW treatment. The experiments show the adverse impact of PTW on B. subtilis–biofilm formation and the phenomenological reaction of B. subtilis.
Imaging in Periprosthetic Joint Infection Diagnosis: A Comprehensive Review
Authors: Hoveidaei Armin , Tavakoli Yasaman , Ramezanpour Mohammad Reza , Omouri-kharashtomi Mahyaar , Taghavi Seyed Pouya , Hoveidaei Amir Human , Conway Janet D.
Published: December 2024

Various imaging methods assist in diagnosing periprosthetic joint infection (PJI). These include radiological techniques such as radiography, computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound (US); as well as advanced nuclear medicine techniques including bone scintigraphy (BS), anti-granulocyte antibody imaging (AGS), leukocyte scintigraphy (LS), and fluorodeoxyglucose positron emission tomography (FDG-PET and FDG-PET/CT). Each imaging technique and radiopharmaceutical has been extensively studied, with unique diagnostic accuracy, limitations, and benefits for PJI diagnosis. This review aims to detail and describe the most commonly used imaging techniques and radiopharmaceuticals for evaluating PJI, focusing particularly on knee and hip arthroplasties.
Crystal Chemistry of a New Mineral-like Phosphate Na6.9Ni2+0.9V3+4.3Al0.8(PO4)8(H2O)2 in the Series of α-CrPO4 Derivatives
Authors: Yakubovich Olga , Kiriukhina Galina , Nesterova Valentina , Volkov Anatoly , Fedotov Stanislav , Dimitrova Olga
Published: December 2024

A novel mineral-like phosphate hydrate Na6.9Ni2+0.9V3+4.3Al0.8(PO4)8(H2O)2 (Z = 2) was obtained under high-temperature hydrothermal conditions by modeling the chemistry of geothermal brines in natural geological solutions. The compound, characterized by scanning electron microscopy and microprobe analysis, possesses an orthorhombic symmetry with the Cccm space group; the unit cell parameters are a = 6.4082(8), b = 19.6813(19), c = 10.5035(11) Å. Here we report its crystal structure studied by low-temperature single crystal X-ray diffraction and discussed as derived from the α-CrPO4 archetype, known for a large range of compounds with promising properties. Three-dimensional continuous migration pathways for Na+ within the structure were found and confirmed by a bond valence energy landscape analysis. The migration barriers turned out to be ~0.44 eV along the a and b directions and ~0.42 eV along the c axis. These values suggest that the compound may be a potential electrode material for sodium-ion batteries.
The Performance Analysis of Pumpable Emulsion Explosives in Narrow-Reef Gold Mines
Authors: Chosi Ramphele , Leeuw Paseka , Nong Matsobane
Published: December 2024

The use of pumpable emulsion explosives in the stopes of narrow-reef gold mines is an emerging practice. This is due to recent developments in the delivery and placement mechanisms of emulsion and gassing agents through portable charging units into small-diameter blastholes. With these developments, this paper outlines the performance of pumpable emulsion explosives in a non-trial basis at two underground gold mines in South Africa, where a combined 33 underground drilling and blasting outcomes were observed in two shafts, where three key performance indicators—namely face advance, powder factor, and fragmentation size distribution—were evaluated. The results indicated that the use of emulsion explosives can enhance the probability of achieving the target face advance, whereas the results of the powder factor are mixed. In one shaft, the actual powder factor of the observed blasts mostly exceeded the planned powder factor, whereas in the other shaft, the latter was largely achieved. Lastly, the results of the fragmentation size distribution analyses are inconclusive; that is, it cannot be conclusively pointed out whether the use of pumpable emulsion explosives can achieve a mean particle fragmentation range of 11.5 cm to 13.5 cm at Shafts A and B.
New Pyridinium Compound from Marine Sediment-Derived Bacterium Bacillus licheniformis S-1
Authors: Wang Han , Wang Yifei , Li Yanjing , Wang Guilin , Shi Ting , Wang Bo
Published: December 2024

The structural diversity of marine natural products is considered a potential resource for the pharmaceutical industry. In our study of marine-derived compounds, one bacterium Bacillus licheniformis S-1 was discovered to have the ability to produce bioactive natural products. After a further chemistry investigation, one novel 4-aminopyridinium derivative, 4-(dimethylamino)-1-(2S-((4hydroxybenzoyl)oxy)propyl)pyridin-1-ium (1), along with 15 known cyclic dipeptides (2–16) were isolated from the bacterium B. licheniformis S-1 derived from a shallow sea sediment. The structures of compounds 1–16 were elucidated through comprehensive NMR spectroscopic and specific optical rotation (OR) data analyses. Compound 6 showed antibacterial activity against Pseudomonas fulva with an MIC value of 50 µg/mL. This is the first study to discover a pyridinium derivative and cyclic dipeptides from B. licheniformis.
Analysis of the Pyrolysis Kinetics, Reaction Mechanisms, and By-Products of Rice Husk and Rice Straw via TG-FTIR and Py-GC/MS
Authors: Lin Li , E Yang , Sun Qiang , Chen Yixuan , Dai Wanning , Bao Zhengrong , Niu Weisheng , Meng Jun
Published: December 2024

This study employed thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), and pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) to characterize and provide insights into the pyrolysis behaviors and by-products of rice husk (RH) and rice straw (RS). The primary pyrolysis range is partitioned into three stages, designated as pseudo-hemicellulose, pseudo-cellulose, and pseudo-lignin pyrolysis, by an asymmetric bi-Gaussian function. The average activation energies of the three pseudo-components of RH were estimated by the Flynn–Wall–Ozawa and Starink methods to be 179.1 kJ/mol, 187.4 kJ/mol, and 239.3 kJ/mol, respectively. The corresponding values for RS were 171.8 kJ/mol, 185.8 kJ/mol, and 203.2 kJ/mol. The results of the model-fitting method indicated that the diffusion model is the most appropriate for describing the pseudo-hemicellulose reaction. The reaction of pseudo-cellulose and pseudo-lignin is most accurately described by a nucleation mechanism. An accelerated heating rate resulted in enhanced pyrolysis performance, with RS exhibiting superior performance to that of RH. RH produces 107 condensable pyrolysis by-products, with ketones, acids, and phenols representing the largest proportion; RS produces 135 species, with ketones, phenols, and alcohols as the main condensable by-products. These high-value added by-products have the potential to be utilized in a variety of applications within the agricultural, bioenergy, and chemical industries.
Study on SHP2 Conformational Transition and Structural Characterization of Its High-Potency Allosteric Inhibitors by Molecular Dynamics Simulations Combined with Machine Learning
Authors: Wujieti Baerlike , Hao Mingtian , Liu Erxia , Zhou Luqi , Wang Huanchao , Zhang Yu , Cui Wei , Chen Bozhen
Published: December 2024

The src-homology 2 domain-containing phosphatase 2 (SHP2) is a human cytoplasmic protein tyrosine phosphatase that plays a crucial role in cellular signal transduction. Aberrant activation and mutations of SHP2 are associated with tumor growth and immune suppression, thus making it a potential target for cancer therapy. Initially, researchers sought to develop inhibitors targeting SHP2’s catalytic site (protein tyrosine phosphatase domain, PTP). Due to limitations such as conservativeness and poor membrane permeability, SHP2 was once considered a challenging drug target. Nevertheless, with the in-depth investigations into the conformational switch mechanism from SHP2’s inactive to active state and the emergence of various SHP2 allosteric inhibitors, new hope has been brought to this target. In this study, we investigated the interaction models of various allosteric inhibitors with SHP2 using molecular dynamics simulations. Meanwhile, we explored the free energy landscape of SHP2 activation using enhanced sampling technique (meta-dynamics simulations), which provides insights into its conformational changes and activation mechanism. Furthermore, to biophysically interpret high-dimensional simulation trajectories, we employed interpretable machine learning methods, specifically extreme gradient boosting (XGBoost) with Shapley additive explanations (SHAP), to comprehensively analyze the simulation data. This approach allowed us to identify and highlight key structural features driving SHP2 conformational dynamics and regulating the activity of the allosteric inhibitor. These studies not only enhance our understanding of SHP2’s conformational switch mechanism but also offer crucial insights for designing potent allosteric SHP2 inhibitors and addressing drug resistance issues.
Advances in Surface-Enhanced Raman Spectroscopy for Therapeutic Drug Monitoring
Authors: Lai Huasheng , Wang Xinlan , Qi Menghan , Huang Hao , Yu Bingqiong
Published: December 2024

Therapeutic drug monitoring (TDM) is pivotal for optimizing drug dosage regimens in individual patients, particularly for drugs with a narrow therapeutic index. Surface-enhanced Raman spectroscopy (SERS) has shown great potential in TDM due to high sensitivity, non-destructive analysis, specific fingerprint spectrum, low sample consumption, simple operation, and low ongoing costs. Due to the rapid development of SERS for TDM, a review focusing on the analytical method is presented to better understand the trends. This review examines the latest advancements in SERS substrates and their applications in TDM, highlighting the innovations in substrate design that enhance detection sensitivity and selectivity. We discuss the challenges faced by SERS for TDM, such as substrate signal reproducibility and matrix interference from complex biological samples, and explore solutions like digital colloid-enhanced Raman spectroscopy, enrichment detection strategies, microfluidic SERS, tandem instrument technologies, and machine learning-enabled SERS. These advancements address the limitations of traditional SERS applications and improve analytical efficiency in TDM. Finally, conclusions and perspectives on future research directions are presented. The integration of SERS with emerging technologies presents a transformative approach to TDM, with the potential to significantly enhance personalized medicine and improve patient outcomes.
Effects of Metamizole, 4-Methylaminoantipyrine, and 4-Aminoantipyrine on LX-2 Liver Cell Line Viability and Apoptosis
Authors: Lupu Georgiana-Iulia , Pall Emoke , Cenariu Mihai , Nan Monica Irina , Andrei Sanda
Published: December 2024

Metamizole (dipyrone) is a non-opioid analgesic widely used in human and veterinary medicine, despite ongoing concerns about its safety due to risks such as agranulocytosis and potential hepatotoxicity. This study investigates the cytotoxic (MTT assay) and pro-apoptotic effects of metamizole and its primary metabolites, 4-methylaminoantipyrine (4-MAA) and 4-aminoantipyrine (4-AA), on the LX-2 liver cell line. These metabolites are implicated in both the therapeutic and adverse effects of the drug. The objective is to elucidate the mechanisms of potential hepatotoxicity, with a focus on cell viability and apoptosis. Metamizole was tested at five concentrations (100, 200, 400, 600, and 1000 µg/mL), while its metabolites were tested at two concentrations (100 and 1000 µg/mL). The results show a dose-dependent decrease in cell viability, with significant reductions at higher concentrations. The greatest cytotoxic effects were observed with 4-AA and 4-MAA, which induced marked apoptosis at 1000 µg/mL. This study concludes that metamizole and its metabolites can cause liver cell damage, underscoring the importance of caution in its clinical use and the need for further research to ensure its safety.
RETRACTED: Yang et al. Capacitive Behavior of Aqueous Electrical Double Layer Based on Dipole Dimer Water Model. Nanomaterials 2023, 13, 16
Authors: Yang Songming , Deng Youer , Zhou Shiqi
Published: December 2024

The journal retracts the article titled “Capacitive Behavior of Aqueous Electrical Double Layer Based on Dipole Dimer Water Model” [...]
Tungsten Diselenide Nanoparticles Produced via Femtosecond Ablation for SERS and Theranostics Applications
Authors: Ushkov Andrei , Dyubo Dmitriy , Belozerova Nadezhda , Kazantsev Ivan , Yakubovsky Dmitry , Syuy Alexander , Tikhonowski Gleb V. , Tselikov Daniil , Martynov Ilya , Ermolaev Georgy , Grudinin Dmitriy , Melentev Alexander , Popov Anton A. , Chernov Alexander , Bolshakov Alexey D. , Vyshnevyy Andrey A. , Arsenin Aleksey , Kabashin Andrei V. , Tselikov Gleb I. , Volkov Valentyn
Published: December 2024

Due to their high refractive index, record optical anisotropy and a set of excitonic transitions in visible range at a room temperature, transition metal dichalcogenides have gained much attention. Here, we adapted a femtosecond laser ablation for the synthesis of WSe2 nanoparticles (NPs) with diameters from 5 to 150 nm, which conserve the crystalline structure of the original bulk crystal. This method was chosen due to its inherently substrate-additive-free nature and a high output level. The obtained nanoparticles absorb light stronger than the bulk crystal thanks to the local field enhancement, and they have a much higher photothermal conversion than conventional Si nanospheres. The highly mobile colloidal state of produced NPs makes them flexible for further application-dependent manipulations, which we demonstrated by creating substrates for SERS sensors.
Evaluation of Battery Management Systems for Electric Vehicles Using Traditional and Modern Estimation Methods
Authors: Mumtaz Noreen Muhammad Talha , Fouladfar Mohammad Hossein , Saeed Nagham
Published: December 2024

This paper presents the development of an advanced battery management system (BMS) for electric vehicles (EVs), designed to enhance battery performance, safety, and longevity. Central to the BMS is its precise monitoring of critical parameters, including voltage, current, and temperature, enabled by dedicated sensors. These sensors facilitate accurate calculations of the state of charge (SOC) and state of health (SOH), with real-time data displayed through an IoT cloud interface. The proposed BMS employs data-driven approaches, like advanced Kalman filters (KF), for battery state estimation, allowing continuous updates to the battery state with improved accuracy and adaptability during each charging cycle. Simulation tests conducted in MATLAB’s Simulink across multiple charging and discharging cycles demonstrate the superior accuracy of the advanced Kalman filter (KF), in handling non-linear battery behaviours. Results indicate that the proposed BMS achieves a significantly lower error margin in SOC tracking, ranging from 0.32% to 1%, compared to traditional methods with error margins up to 5%. These findings underscore the importance of integrating robust sensor systems in BMSs to optimise EV battery management, reduce maintenance costs, and improve battery sustainability.
Effects of Metabolites Derived from Guava (Psidium guajava L.) Leaf Extract Fermented by Limosilactobacillus fermentum on Hepatic Energy Metabolism via SIRT1-PGC1α Signaling in Diabetic Mice
Authors: Jeon Sohyun , Lee Heaji , Kim Sun-Yeou , Lee Choong-Hwan , Lim Yunsook
Published: December 2024

Background/Objectives: Type 2 diabetes mellitus (T2DM) is considered a serious risk to public health since its prevalence is rapidly increasing worldwide despite numerous therapeutics. Insulin resistance in T2DM contributes to chronic inflammation and other metabolic abnormalities that generate fat accumulation in the liver, eventually leading to the progression of metabolic dysfunction-associated fatty liver disease (MAFLD). Recently, the possibility that microbial-derived metabolites may alleviate MAFLD through enterohepatic circulation has emerged, but the underlying mechanism remains unclear. In this research, we utilized metabolites obtained from the fermentation of guava leaf extract, which is well-known for its antidiabetic activity, to investigate their effects and mechanisms on MAFLD. Methods: Diabetes was induced by a high-fat diet and streptozotocin injection (80 mg/kg body weight) twice in mice. Subsequently, mice whose fasting blood glucose levels were measured higher than 300 mg/dL were administered with metabolites of Limosilactobacillus fermentum (LF) (50 mg/kg/day) or guava leaf extract fermented by L. fermentum (GFL) (50 mg/kg/day) by gavage for 15 weeks. Results: GFL supplementation mitigated hyperglycemia and hepatic insulin resistance. Moreover, GFL regulated abnormal hepatic histological changes and lipid profiles in diabetic mice. Furthermore, GFL enhanced energy metabolism by activating the sirtuin1 (SIRT1)/proliferator-activated receptor γ coactivator 1α (PGC1α)/peroxisome proliferator-activated receptor (PPAR)-α pathway in diabetic mice. Meanwhile, GFL supplementation suppressed hepatic inflammation in diabetic mice. Conclusions: Taken together, the current study elucidated that GFL could be a potential therapeutic to ameliorate hyperglycemia and hepatic steatosis by improving SIRT1/PGC-1α/ PPAR-α-related energy metabolism in T2DM.
Effects of Longer-Term Mixed Nut Consumption on Lipoprotein Particle Concentrations in Older Adults with Overweight or Obesity
Authors: Nijssen Kevin M. R. , Chavez-Alfaro Marco A. , Joris Peter J. , Plat Jogchum , Mensink Ronald P.
Published: December 2024

Background: Recently, we reported that longer-term mixed nut intake significantly reduced serum total and low-density lipoprotein (LDL)-cholesterol, but these markers may not fully capture lipoprotein-related cardiovascular disease (CVD) risk. Objectives: This randomized, controlled, single-blinded, crossover trial in older adults with overweight or obesity examined the effects of longer-term mixed nut consumption on lipoprotein particle size, number, and lipid distribution. Methods: Twenty-eight participants (aged 65 ± 3 years; BMI 27.9 ± 2.3 kg/m2) completed two 16-week periods (control [no nuts] vs. mixed nuts (60 g/day: 15 g of walnuts, pistachios, cashews, and hazelnuts), separated by an 8-week washout. Plasma lipoprotein particle numbers, sizes, and lipid distributions across subclasses were analyzed using high-throughput nuclear magnetic resonance (NMR) spectroscopy. Results: Mixed nut consumption significantly reduced Apolipoprotein B (ApoB) concentrations (−0.07 g/L; p = 0.009), total cholesterol (−0.27 mmol/L; p = 0.047), non-HDL cholesterol (−0.28 mmol/L; p = 0.022), and total triacylglycerol (TAG) (−0.27 mmol/L; p = 0.008). Total very large-density lipoprotein (VLDL) particle numbers decreased by 24 nmol/L (p < 0.001), with reductions observed across all VLDL subclasses. Total LDL particle numbers (p = 0.044), specifically intermediate-density lipoprotein (IDL) (p = 0.002) and large LDL particles (p = 0.015), were also reduced, while HDL particle numbers and sizes were unaffected. The mixed nut intervention significantly reduced cholesterol concentrations across all VLDL subclasses and IDL (all p < 0.01), with no changes in LDL or HDL subclasses. TAG concentrations showed reductions across all lipoprotein subclasses (all p < 0.05). Conclusions: Longer-term mixed nut consumption may lower CVD risk in older adults and favorable shifts in apoB-containing lipoprotein subclasses towards a less atherogenic profile.
Food Behavior and Lifestyle Among Students: The Influence of the University Environment
Authors: Avram Calin , Nyulas Victoria , Onisor Danusia , Georgescu Ion Mihai , Szakacs Julianna , Ruta Florina
Published: December 2024

Introduction: A key element for a healthy lifestyle in the formative years of a young adult is a balanced, healthy, individualized diet. Aim: The aim of this study was to analyze the eating behavior and lifestyles of students at a university in Romania, as well as the extent to which the study program interferes with these factors. Methods: A retrospective study was performed, with the data being collected through an online questionnaire via the Google Forms platform; data collection for the current study was carried out in November 2024 during a student session. Results: Out of the total of 501 questionnaires collected, 416 were completed by women and 85 by men. It can be observed that 57.65% of men (49 participants) eat three meals a day compared to 53.13% of women (221 participants). The university program influenced the students’ meal schedules for 59.88% (n = 300) of participants, while 30.94% (n = 155) declared that they never have an ordered meal schedule. For 39.92% of students, the exam period has an influence on the meal schedule, including an increase the intake of sweet foods (59.88%), and some declared that they forget to eat (33.93%). Conclusions: The food and lifestyle behaviors in the studied group show health risk elements, especially an increase in tobacco consumption, a lack of regular meal schedules, and the consumption of an excess of unhealthy snacks. It was found that intensive study schedules may intensify this behavior.
Musa paradisiaca L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model
Authors: Gadelha Francisco Allysson Assis Ferreira , Cavalcanti Raquel Fragoso Pereira , Vieira Cosmo Isaias Duvirgens , De Oliveira Joao Batista , De Lima Louíse Mangueira , Alves Adriano Francisco , Pessoa Matheus Marley Bezerra , Batista Leônia Maria , Dejani Naiara Naiana , Piuvezam Marcia Regina
Published: December 2024

Background/Objectives: Acute lung injury (ALI) is an inflammatory disorder affecting patients in intensive care with high mortality. No specific pharmacological treatment is available. Musa paradisiaca L. (banana) is a cosmopolitan plant, and homemade syrup from its inflorescence is used in many countries to treat pulmonary inflammation. Therefore, this study analyzed the hydroalcoholic extract (HEM) of the inflorescence on the ALI experimental model. Methods: Swiss mice were challenged with lipopolysaccharide and treated with HEM after 1, 24, and 48 h (five animals/group, three times). Results: The HEM-treated ALI mice presented a decrease in neutrophil migration in the bronchoalveolar lavage fluid (BALF), in the alveolar region, and in the blood, correlating to downregulation of CD18 expression. The HEM treatment also reduced the protein concentration in the BALF, caused lung edema formation, impaired NF-κB activation via inhibition of TLR4 signaling pathway, and decreased IL-1β, TNF-α production, free DNA release, and myeloperoxidase (MPO) activity. However, the extract induced an increased IL-10 in the BALF. Conclusions: Therefore, HEM’s anti-inflammatory and immunomodulatory activities in ALI mice are by deactivating neutrophils by decreasing CD18 receptor, free DNA release, and MPO activity and inducing IL-10 production. Thus, this study supports the use of banana inflorescence in folk medicine and suggests its rational use to develop a phytomedicine to treat pulmonary inflammation.
Innovative Solid Lipid Nanoparticle-Enriched Hydrogels for Enhanced Topical Delivery of L-Glutathione: A Novel Approach to Anti-Ageing
Authors: Liu Mengyang , Sharma Manisha , Lu Guoliang , Zhang Zhiwen , Song Wenting , Wen Jingyuan
Published: December 2024

Background: Skin ageing, driven predominantly by oxidative stress from reactive oxygen species (ROS) induced by environmental factors like ultraviolet A (UVA) radiation, accounts for approximately 80% of extrinsic skin damage. L-glutathione (GSH), a potent antioxidant, holds promise in combating UVA-induced oxidative stress. However, its instability and limited penetration through the stratum corneum hinder its topical application. This study introduces a novel solid lipid nanoparticle (SLN)-enriched hydrogel designed to enhance GSH stability, skin penetration, and sustained release for anti-ageing applications. Methods: GSH-loaded SLNs were prepared via a double-emulsion technique and optimized using factorial design. These SLNs were incorporated into 1–3% (w/v) Carbopol hydrogels to produce a semi-solid formulation. The hydrogel’s characteristics, including morphology, mechanical and rheological properties, drug release, stability, antioxidant activity, cytotoxicity, and skin penetration, were evaluated. Results: SEM and FTIR confirmed the uniform dispersion of SLNs within the hydrogel. The formulation exhibited desirable properties, including gel strength (5.1 ± 0.5 g), spreadability (33.6 ± 1.9 g·s), pseudoplasticity, and elasticity. In vitro studies revealed a biphasic GSH release profile, with sustained release over 72 h and over 70% cumulative release. The hydrogel significantly improved antioxidant capacity, protecting human fibroblasts from UVA-induced oxidative stress and enhancing cell viability. Stability studies indicated that 4 °C was optimal for storage over three months. Notably, the hydrogel enhanced GSH penetration through the stratum corneum by 3.7-fold. Conclusions: This SLN-enriched hydrogel effectively improves GSH topical delivery and antioxidant efficacy, providing a promising platform for anti-ageing and other bioactive compounds with similar delivery challenges.
Development and Characterization of Trihexyphenidyl Orodispersible Minitablets: A Challenge to Fill the Therapeutic Gap in Neuropediatrics
Authors: Olivera Camila , Boscolo Oriana , Dobrecky Cecilia , Ortega Claudia A. , Favier Laura S. , Cianchino Valeria A. , Flor Sabrina , Lucangioli Silvia
Published: December 2024

Background: Trihexyphenidyl (THP) has been widely used for over three decades as pediatric pharmacotherapy in patients affected by segmental and generalized dystonia. In order to achieve effective and safe pharmacotherapy for this population, new formulations are needed. Objective: The aim of this work is the development of trihexyphenidyl orodispersible minitablets (ODMTs) for pediatric use. Methods: Six different excipients were tested as diluents. The properties of powder mixtures were evaluated before direct compression and pharmacotechnical tests were performed on the final formulation. The determination of the API content, uniformity of dosage, and physicochemical stability studies were analyzed by an HPLC-UV method. Results: The developed ODMTs met pharmacopeia specifications for content, hardness, friability, disintegration, and dissolution tests. The physicochemical stability study performed over 18 months shows that API content remains within 90.0–110.0% at least for this period. Conclusions: These ODMTs will allow efficient, safe, and high-quality pharmacotherapy.
Selective Laser Sintering 3D Printing of Carvedilol Tablets: Enhancing Dissolution Through Amorphization
Authors: Pešić Nikola , Ivković Branka , Barudžija Tanja , Grujić Branka , Ibrić Svetlana , Medarević Djordje
Published: December 2024

Background/Objectives: Selective laser sintering (SLS) is one of the most promising 3D printing techniques for pharmaceutical applications as it offers numerous advantages, such as suitability to work with already approved pharmaceutical excipients, the elimination of solvents, and the ability to produce fast-dissolving, porous dosage forms with high drug loading. When the powder mixture is exposed to elevated temperatures during SLS printing, the active ingredients can be converted from the crystalline to the amorphous state, which can be used as a strategy to improve the dissolution rate and bioavailability of poorly soluble drugs. This study investigates the potential application of SLS 3D printing for the fabrication of tablets containing the poorly soluble drug carvedilol with the aim of improving the dissolution rate of the drug by forming an amorphous form through the printing process. Methods: Using SLS 3D printing, eight tablet formulations were produced using two different powder mixtures and four combinations of experimental conditions, followed by physicochemical characterization and dissolution testing. Results: Physicochemical characterization revealed that at least partial amorphization of carvedilol occurred during the printing process. Although variations in process parameters were minimal, higher temperatures in combination with lower laser speeds appeared to facilitate a greater degree of amorphization. Ultimately, the partial conversion to the amorphous form significantly improved the dissolution of carvedilol compared to its pure crystalline form. Conclusions: Obtained results suggest that the SLS 3D printing technique can be effectively used to convert poorly water-soluble drugs to their amorphous state, thereby improving solubility and bioavailability.
Long-Term Therapeutic Effects of 225Ac-DOTA-E[c(RGDfK)]2 Induced by Radiosensitization via G2/M Arrest in Pancreatic Ductal Adenocarcinoma
Authors: Yoshimoto Mitsuyoshi , Washiyama Kohshin , Ohnuki Kazunobu , Doi Ayano , Inokuchi Miki , Kojima Motohiro , Miller Brian W. , Yoshii Yukie , Inaki Anri , Fujii Hirofumi
Published: December 2024

Background: Alpha radionuclide therapy has emerged as a promising novel strategy for cancer treatment; however, the therapeutic potential of 225Ac-labeled peptides in pancreatic cancer remains uninvestigated. Methods: In the cytotoxicity study, tumor cells were incubated with 225Ac-DOTA-RGD2. DNA damage responses (γH2AX and 53BP1) were detected using flowcytometry or immunohistochemistry analysis. Biodistribution and therapeutic studies were carried out in BxPC-3-bearing mice. Results: 225Ac-DOTA-RGD2 demonstrated potent cytotoxicity against cells expressing αvβ3 or αvβ6 integrins and induced G2/M arrest and γH2AX expression as a marker of double-stranded DNA breaks. 225Ac-DOTA-RGD2 (20, 40, 65, or 90 kBq) showed favorable pharmacokinetics and remarkable tumor growth inhibition without severe side effects in the BxPC-3 mouse model. In vitro studies revealed that 5 and 10 kBq/mL of 225Ac-DOTA-RGD2 swiftly induced G2/M arrest and elevated γH2AX expression. Furthermore, to clarify the mechanism of successful tumor growth inhibition for a long duration in vivo, we investigated whether short-term high radiation exposure enhances radiation sensitivity. Initially, a 4 h induction treatment with 5 and 10 kBq/mL of 225Ac-DOTA-RGD2 enhanced both cytotoxicity and γH2AX expression with 0.5 kBq/mL of 225Ac-DOTA-RGD2 compared to a treatment with only 0.5 kBq/mL of 225Ac-DOTA-RGD2. Meanwhile, the γH2AX expression induced by 5 or 10 kBq/mL of 225Ac-DOTA-RGD2 alone decreased over time. Conclusions: These findings highlight the potential of using 225Ac-DOTA-RGD2 in the treatment of intractable pancreatic cancers, as its ability to induce G2/M cell cycle arrest enhances radiosensitization, resulting in notable growth inhibition.
Silicon Nitride Spot-Size Converter with Coupling Loss < 1.5 dB for Both Polarizations at 1W Optical Input
Authors: Zhang Enge , Zhang Yu , Zhang Lei , Yang Xu
Published: December 2024

Microwave photonics (MWP) applications often require a high optical input power (>100 mW) to achieve an optimal signal-to-noise ratio (SNR). However, conventional silicon spot-size converters (SSCs) are susceptible to high optical power due to the two-photon absorption (TPA) effect. To overcome this, we introduce a silicon nitride (SiN) SSC fabricated on a silicon-on-insulator (SOI) substrate. When coupled to a tapered fiber with a 4.5 μm mode field diameter (MFD), the device exhibits low coupling losses of <0.9 dB for TE modes and <1.4 dB for TM modes at relatively low optical input power. Even at a 1W input power, the additional loss is minimal, at approximately 0.1 dB. The versatility of the SSC is further demonstrated by its ability to efficiently couple to fibers with MFDs of 2.5 μm and 6.5 μm, maintaining coupling losses below 1.5 dB for both polarizations over the entire C-band. This adaptability to different mode diameters makes the SiN SSC a promising candidate for future electro-optic chiplets that integrate heterogeneous materials such as III-V for gain and lithium niobate for modulation with the SiN-on-SOI for all other functions using advanced packaging techniques.
Disinfection Strategies for Poly(methyl methacrylate): Method Sequence, Solution Concentration, and Intraoral Temperature on Antimicrobial Activity
Authors: Kamio Ana Beatriz Sato , da Silva Barboza Andressa , da Silva Maria Eduarda Broering , Soto Artur Ferronato , de Andrade Juliana Silva Ribeiro , Duque Thais Mageste , da Cruz Ariadne Cristiane Cabral , Mazzon Ricardo Ruiz , Badaró Maurício Malheiros
Published: December 2024

This study aimed to evaluate the antimicrobial effectiveness of different disinfection protocols for dentures by combining methods, varying intervention sequences, sodium hypochlorite (NaOCl) concentrations (0.1% and 0.25%), and post-exposure to intraoral temperature. The heat-polymerized poly(methylmethacrylate) (PMMA) was divided into groups (n = 15): control (C, distilled water immersion), B (brushing), I0.1% and I0.25% (isolated NaOCl immersion), B + I0.1% and B + I0.25% (brushing followed by immersion), I + B0.1% and I + B0.25% (immersion followed by brushing), and B + I0.1% + T and B + I0.25% + T (brushing, NaOCl immersion, and overnight exposure to 35 °C ± 2 °C). The post-disinfection exposure to intraoral temperature simulated the denture use during sleeping time. Quantitative evaluation was performed by colony-forming unit (CFU/mL) counting of C. albicans and qualitative analysis by scanning electron microscopy (SEM) images. Data were processed by one-way ANOVA with Tukey’s post-hoc test to compare different protocols at the same concentration and among groups (α ≤ 0.05). Applying 0.25% NaOCl in associated protocols, the intervention sequence was no different (B + I and I + B) and caused the lowest C. albicans counts. The 0.1% NaOCl lost part of its action when the immersion method started the protocols. B + I0.25%, I0.25% + B, and B + I0.1% had similar antimicrobial efficacy, but the intraoral temperature (B + I + T) reduced the efficacy of these protocols, regardless of NaOCl concentration. Residual biofilm recolonization was also detected in SEM images. In conclusion, all the combinations between mechanical and chemical methods using 0.25% NaOCl were the most effective against C. albicans. The antimicrobial efficacy of NaOCl at 0.1% changes depending on the intervention sequence. The intraoral temperature influenced the C. albicans recolonization after the disinfection protocols.
Prospects on Mixed Tutton Salt (K0.86Na0.14)2Ni(SO4)2(H2O)6 as a Thermochemical Heat Storage Material
Authors: Marques Jacivan V. , Oliveira Neto João G. de , Silva Neto Otávio C. da , dos Santos Adenilson O. , Lang Rossano
Published: December 2024

In this paper, a novel mixed Tutton salt (K0.86Na0.14)2Ni(SO4)2(H2O)6 was successfully synthesized as a single crystal and evaluated as a thermochemical heat storage material. Its thermal and thermochemical properties were correlated with the structure, which was determined by powder X-ray diffraction using the Le Bail and Rietveld methods. The elemental ratio between the K+ and Na+ monovalent cations was established by energy-dispersive X-ray spectroscopy. Similar compounds such as Na2Ni(SO4)2(H2O)4 and K2Ni(SO4)2(H2O)6 were also synthesized and used for structural comparisons. The (K0.86Na0.14)2Ni(SO4)2(H2O)6 salt crystallizes in monoclinic symmetry with the P21/c-space group, typical of hexahydrate crystals from the Tutton salt family. The lattice parameters closely resemble those of K2Ni(SO4)2(H2O)6. A comprehensive analysis of the intermolecular contacts, based on Hirshfeld surfaces and 2D fingerprint mappings, revealed that the primary interactions are hydrogen bonds (H···O/O···H) and ion-dipole interactions (K/Na···O/O···Na/K). The unit cell exhibits minimal void space, accounting for only 0.2%, indicative of strong atomic packing. The intermolecular molecular and atomic packing are important factors influencing crystal lattice stabilization and thermal energy supplied to release crystallographic H2O. The thermal stability of mixed Tutton salt ranges from 300 K to 365 K. Under the dehydration of its six H2O molecules, the dehydration reaction enthalpy reaches 349.8 kJ/mol, yielding a thermochemical energy storage density of 1.79 GJ/m3. With an H2O desorption temperature ≤393 K and a high energy storage density ≥1.3 GJ/m3 (criteria established for applications at the domestic level), the (K0.86Na0.14)2Ni(SO4)2(H2O)6 shows potential as a thermochemical material for small-sized heat batteries.
Location Distribution of Lightning Localization Monitoring Stations Integrating 3D Monitoring and PSO Algorithm
Authors: Han Yiming , He Bin , Shu Hongchun
Published: December 2024

The growth of the modern information technology sector and the safe use of electrical equipment depend on accurate lightning localization monitoring. However, along with the increase in the number of lightning localization equipment and monitoring stations, the unreasonable station location distribution increases the risk of error in lightning localization monitoring. Therefore, the study integrates three-dimensional cross-location lightning monitoring and particle swarm algorithm to optimize the multi-station location distribution of lightning localization monitoring. In the simulation experiment of the four-station lightning monitoring system, compared with the traditional Y-distribution method, the particle swarm optimization method reduced the lightning positioning error by 45.8%. Meanwhile, in the practical application analysis, the positioning error of the proposed method in the horizontal and vertical axes was basically less than 0.4 km, which was the smallest compared with other models. The experiment proves that the lightning localization monitoring station distribution method fusing 3D localization and PSO algorithm constructed by the research has good location distribution performance and can be effectively applied to the layout of the current multi-station lightning monitoring system.
Tool Wear State Monitoring in Titanium Alloy Milling Based on Wavelet Packet and TTAO-CNN-BiLSTM-AM
Authors: Yang Zongshuo , Li Li , Zhang Yunfeng , Jiang Zhengquan , Liu Xuegang
Published: December 2024

To effectively monitor the nonlinear wear variation of tools during the processing of titanium alloys, this study proposes a hybrid deep neural network fault diagnosis model that integrates the triangulation topology aggregation optimizer (TTAO), convolutional neural network (CNN), bidirectional long short-term memory network (BiLSTM), and attention mechanism (AM). Firstly, vibration signals from the machine tool spindle are acquired and subjected to the wavelet packet transform (WPT) to extract multi-frequency band energy features as model inputs. Then, the CNN and BiLSTM modules capture the features and temporal relationships of the input signals. Finally, introduction of the AM, combined with the TTAO algorithm, automatically extracts deep features, overcoming issues such as local optima and slow convergence in traditional neural networks, thereby enhancing the accuracy and efficiency of tool wear state recognition. The experimental results demonstrate that the proposed model achieves an average accuracy rate of 98.649% in predicting tool wear states, outperforming traditional backpropagation (BP) networks and standard CNN models.