bluuebunny/crossref_metadata_2025 huggingface dataset:
Complements from the Male Reproductive Tract: A Scoping Review
Authors: Washburn Rachel L.
Published: January 2024

The organs of the male reproductive tract, including the testis, epididymis, prostate, seminal vesicles, and semen, must provide an immunoregulatory environment conducive to germ cell viability and successful fertilization. Many immune components, such as immune cells, have been investigated regarding reproductive immunology and function; however, the investigation of the role of complement in this system has only more recently been gaining traction in research. This review focuses on complement in the male reproductive tract, with the goal of compiling information currently known about complement components detected in male reproductive organs and identifying areas in need of further research. Considering the recent and upcoming research about the noncanonical functions of complement, this information is relevant and applicable in the fields of reproductive immunology, fertility, and immune regulation.
The Association between Coagulation and Atrial Fibrillation
Authors: Rafaqat Saira , Gluscevic Sanja , Patoulias Dimitrios , Sharif Saima , Klisic Aleksandra
Published: January 2024

The existing literature highlights the presence of numerous coagulation factors and markers. Elevated levels of coagulation factors are associated with both existing and newly diagnosed cases of atrial fibrillation (AF). However, this article summarizes the role of coagulation in the pathogenesis of AF, which includes fibrinogen and fibrin, prothrombin, thrombomodulin, soluble urokinase plasminogen activator receptor, von Willebrand factor, P-selectin, D-dimer, plasminogen activator inhibitor-1, and platelet activation. Coagulation irregularities play a significant role in the pathogenesis of AF.
Evaluating the Healing Potential of J-Plasma Scalpel-Created Surgical Incisions in Porcine and Rat Models
Authors: Elmore Lilith , Minissale Nicholas J. , Israel Lauren , Katz Zoe , Safran Jordan , Barba Adriana , Austin Luke , Schaer Thomas P. , Freeman Theresa A.
Published: January 2024

Cold atmospheric plasma devices generate reactive oxygen and nitrogen species that can be anti-microbial but also promote cell migration, differentiation, and tissue wound healing. This report investigates the healing of surgical incisions created using cold plasma generated by the J-Plasma scalpel (Precise Open handpiece, Apyx Medical, Inc.) compared to a steel scalpel in in vivo porcine and rat models. The J-Plasma scalpel is currently FDA approved for the delivery of helium plasma to cut, coagulate, and ablate soft tissue during surgical procedures. To our knowledge, this device has not been studied in creating surgical incisions but only during deeper dissection and hemostasis. External macroscopic and histologic grading by blinded reviewers revealed no significant difference in wound healing appearance or physiology in incisions created using the plasma scalpel as compared with a steel blade scalpel. Incisions created with the plasma scalpel also had superior hemostasis and a reduction in tissue and blood carryover. Scanning electron microscopy (SEM) and histology showed collagen fibril fusion occurred as the plasma scalpel incised through the tissue, contributing to a sealing effect. In addition, when bacteria were injected into the dermis before incision, the plasma scalpel disrupted the bacterial membrane as visualized in SEM images. External macroscopic and histologic grading by blinded reviewers revealed no significant difference in wound healing appearance or physiology. Based on these results, we propose additional studies to clinically evaluate the use of cold plasma in applications requiring hemostasis or when an increased likelihood of subdermal pathogen leakage could cause surgical site infection (i.e., sites with increased hair follicles).
Is It Time to Alter the Standard of Care for Iron Deficiency/Iron Deficiency Anemia in Reproductive-Age Women?
Authors: Bellad Mrutyunjaya B. , Patted Anmol , Derman Richard J.
Published: January 2024

Two billion people worldwide suffer from anemia, with reproductive-age women being disproportionately affected. Iron plays a crucial role in cellular function and impacts cognition, physical function, and quality of life. Iron deficiency (ID) and iron deficiency anemia (IDA) are associated with adverse effects on pregnancy and fetal development. Oral iron supplementation has been the standard treatment for decades, often producing sub-optimal outcomes. Many babies are still being born with ID and suffer adverse sequelae due to inadequate iron levels in the mothers. Is it time to consider a broad scale-up of parenteral iron as a new standard of care?
Integrated Proteogenomic Analysis Reveals Distinct Potentially Actionable Therapeutic Vulnerabilities in Triple-Negative Breast Cancer Subtypes
Authors: Kaur Pushpinder , Ring Alexander , Porras Tania B. , Zhou Guang , Lu Janice , Kang Irene , Lang Julie E.
Published: January 2024

Triple-negative breast cancer (TNBC) is characterized by an aggressive clinical presentation and a paucity of clinically actionable genomic alterations. Here, we utilized the Cancer Genome Atlas (TCGA) to explore the proteogenomic landscape of TNBC subtypes to see whether genomic alterations can be inferred from proteomic data. We found only 4% of the protein level changes are explained by mutations, while 21% of the protein and 35% of the transcriptomics changes were determined by copy number alterations (CNAs). We found tighter coupling between proteome and genome in some genes that are predicted to be the targets of drug inhibitors, including CDKs, PI3K, tyrosine kinase (TKI), and mTOR. The validation of our proteogenomic workflow using mass spectrometry Clinical Proteomic Tumor Analysis Consortium (MS-CPTAC) data also demonstrated the highest correlation between protein–RNA–CNA. The integrated proteogenomic approach helps to prioritize potentially actionable targets and may enable the acceleration of personalized cancer treatment.
Glass Composition for Coating and Bonding of Polycrystalline Spinel Ceramic Substrates
Authors: Hormadaly Jacob
Published: January 2024

Design considerations of the lead-based glass composition was broadened beyond the two known criteria of matched index of refraction and thermal coefficient of expansion to include previous studies of thick film materials. Five criteria for the glass-design composition were used: matched index of refraction and thermal coefficient of expansion, components (MgO and Al2O3) to slow down dissolution of spinel (MgAl2O4) into the glass, non-crystallizing glass in a broad temperature range and glass with good chemical durability. Synthesis and characterization of glass, glass paste preparation and its application to spinel substrates to form coating and bonding and optical characterizations in the UV, VIS and IR of coated, uncoated, and bonded spinel substrates of two commercial sources are described. Enhancement of transmittance exceeding the theoretical value of polished spinel was found for the first time when glass coating was applied to a ground face of semi-polished spinel.
Quality and Diagnosis on the Lateral View of Pediatric Upper Gastro-Intestinal Series
Authors: Venkatakrishna Shyam Sunder B. , Elsingergy Mohamed , Calle-Toro Juan S. , Dennis Rebecca , Otero Hansel J. , Andronikou Savvas
Published: January 2024

Background: The standard imaging technique for the diagnosis of intestinal malrotation remains the upper gastro-intestinal series (UGIS). The lateral view is promoted as important for making a diagnosis. For this, the lateral view should be of adequate quality, and radiologists must know the normal appearance as well as the appearance of duodenal variants, as misdiagnosis may lead to unnecessary surgery. Objective: We aimed to evaluate the quality, findings including the prevalence of the ”descending staircase” configuration and its correspondence to a diagnosis of duodenum redundum. Materials and Methods: This was a retrospective study and was conducted in a large tertiary children’s hospital in the United States. A retrospective review of UGI fluoroscopy exams in children aged ≤ 18 years between January and December 2018 was performed by a pediatric radiologist. First, the lateral view images/cine-loops were assessed independently, followed by the anteroposterior (AP) view. The studies which were designated to have an adequate lateral view were evaluated for configuration of the duodenum and recorded as: normal, abnormal, or normal variant. Also, the presence of a descending staircase configuration was correlated with an AP view for a diagnosis of duodenum redundum. Results: A total of 26 children (26%) (males:16; females:10) with age range 0 to 16 years had adequate lateral views during UGI exams for inclusion. Of the 26, 18 (69%) were reported as normal, 7 (27%) were reported as having a descending staircase and 1 (4%) was reported as abnormal. The AP view demonstrated 2 abnormal studies (1 malrotation and 1 non-rotation), 6 duodenum redundum and 18 normal exams. The one abnormal lateral duodenum was confirmed as a non-rotation on AP view; the second patient with an abnormal AP view had a normal appearance on the lateral view. Conclusions: A total of 26% of UGI studies had adequate lateral views for interpretation. Of these, nearly a quarter (23%) demonstrated the descending stair-case sign corresponding to a diagnosis of duodenum redundum on the AP view. If the lateral view had been used alone, there would have been a missed diagnosis in one patient.
Molecular Characterization and Expression Changes of the bcl2l13 Gene in Response to Hypoxia in Megalobrama amblycephala
Authors: Zhang Axin , Guo Xuefei , Bao Kaikai , Wu Danyang , Liu Hong , Gao Zexia , Wang Huanling
Published: January 2024

Hypoxia is a unique environmental stress, which not only reflects the insufficient oxygen supply of cells and tissues, but also occurs in various physiological and pathological environments. Mitophagy as a selective autophagy can recover and utilize damaged organelles and misfolded proteins to ensure normal cell functions and promote cell survival. Bcl2l13 (B-cell lymphoma-2 like 13) is reported to induce mitophagy as a functional mammalian homolog of Atg32. However, the function of the bcl2l13 gene is still unclear in fish. Here the sequence and structure of the bcl2l13 gene in Megalobrama amblycephala were identified and showed that bcl2l13 contained an open reading frame (ORF) of 1458 bp for encoding 485 aa. Amino acid sequence analysis indicated that Bcl2l13, as a typical anti-apoptotic protein of the Bcl2 family, contained four BH domains, one BHNo domain, and one TM domain. Further study showed that Bcl2l13 was mainly located in the mitochondria, while its localization was changed within the whole cell after the TM domain was deleted. Real-time PCR analysis revealed that bcl2l13 showed higher expression levels in early embryos. After hypoxia treatment, the mRNA levels of the bcl2l13 and autophagy-related genes were significantly up-regulated in most detected tissues, and the bcl2l13 transcription was regulated by Hif-1α mediated pathway. Additionally, the transcription activity of the bcl2l13 promoter was further analyzed using luciferase reporter assays and showed the highest activity in the promoter region from −475 to +111. These results indicated that bcl2l13 may play important roles in embryogenesis and hypoxia mediated autophagy in fish.
Unraveling Arrhythmias with Graph-Based Analysis: A Survey of the MIT-BIH Database
Authors: Alinsaif Sadiq
Published: January 2024

Cardiac arrhythmias, characterized by deviations from the normal rhythmic contractions of the heart, pose a formidable diagnostic challenge. Early and accurate detection remains an integral component of effective diagnosis, informing critical decisions made by cardiologists. This review paper surveys diverse computational intelligence methodologies employed for arrhythmia analysis within the context of the widely utilized MIT-BIH dataset. The paucity of adequately annotated medical datasets significantly impedes advancements in various healthcare domains. Publicly accessible resources such as the MIT-BIH Arrhythmia Database serve as invaluable tools for evaluating and refining computer-assisted diagnosis (CAD) techniques specifically targeted toward arrhythmia detection. However, even this established dataset grapples with the challenge of class imbalance, further complicating its effective analysis. This review explores the current research landscape surrounding the application of graph-based approaches for both anomaly detection and classification within the MIT-BIH database. By analyzing diverse methodologies and their respective accuracies, this investigation aims to empower researchers and practitioners in the field of ECG signal analysis. The ultimate objective is to refine and optimize CAD algorithms, ultimately culminating in improved patient care outcomes.
The Feasibility and Acceptability of a Data Capture Methodology in Pediatric Cancer Patients Treated with Targeted Agents and Immunotherapies
Authors: Sadak Karim Thomas , Aremu Taiwo Opeyemi , Buttar Seah , Ly Daniel Van , Weigel Brenda , Neglia Joseph P.
Published: January 2024

As childhood cancer treatments have improved to include new and innovative agents, the need for more advanced monitoring of their long-term effects and related research has increased. This has resulted in a need for evidence-based research methodologies for the longitudinal care of childhood cancer patients treated with targeted agents and immunotherapies. The rationale for this pilot study was to determine the feasibility and acceptability of a data capture methodology for pediatric, adolescent, and young adult cancer patients treated with targeted agents and immunotherapy as there is little research to inform this delivery of care. Data were collected from thirty-two patients and two providers for descriptive statistics and thematic analyses. Feasibility was characterized by expected participant attrition. Key drivers of acceptability were (1) providers’ language and clarity of communication and (2) convenient participation requirements. Long-term follow-up research practices developed with input from key stakeholders, including patients, caregivers, and providers, can lead to acceptable and feasible research protocols that optimize successful participant recruitment. These evidence-based research practices can result in high participant satisfaction and can be implemented as program development initiatives across centers caring for childhood cancer survivors.
A Real-World Retrospective Analysis of the Management of Advanced Urothelial Carcinoma in Canada
Authors: Moria Feras A. , Park Changsu L. , Eigl Bernhard J. , Macfarlane Robyn , Pavic Michel , Saleh Ramy R.
Published: January 2024

Locally advanced or metastatic urothelial carcinoma (aUC) presents a significant challenge with high mortality rates. Platinum-based chemotherapy remains the established frontline standard of care, and a switch-maintenance strategy with immunotherapy has now emerged as a new standard for aUC patients without disease progression, following initial platinum therapy. Examining the treatment patterns is imperative, given the evolving therapeutic landscape. In this study, we conducted a retrospective medical chart review of 17 Canadian oncologists treating patients with aUC to assess unmet needs in Canadian aUC patient care. Data from 146 patient charts were analyzed, revealing important clinical insights about the management of aUC. A substantial proportion of patients (53%) presented with de novo metastatic disease, which was possibly influenced by pandemic-related care disruptions. Variability was evident in the cisplatin eligibility criteria, with a majority (70%) of oncologists utilizing a 50 mL/min threshold. Most favored four cycles of platinum-based chemotherapy to spare the bone marrow for future therapies and prevent patient fatigue. Notably, some eligible patients were kept under surveillance rather than receiving maintenance therapy, suggesting a potential gap in awareness regarding evidence-based recommendations. Furthermore, managing treatment-related adverse events was found to be one of the biggest challenges in relation to maintenance immunotherapy. In conclusion, our findings provide the first comprehensive overview of aUC treatment patterns in Canada following the approval of maintenance immunotherapy, offering insights into the decision-making process and underscoring the importance of evidence-based guidelines in aUC patient management.
A Survey of Bacteria and Fungi Associated with Leaves, Rhizophylls, and Vesicles of the Carnivorous Plant Genlisea hispidula (Lentibulariaceae)
Authors: Raudabaugh Daniel B. , Aime M. Catherine
Published: January 2024

Carnivorous plants capture, digest, and absorb prey via specialized structures such as bladders, pitchers, and other modified leaf traps. Studies have shown that not all carnivorous plants produce digestive enzymes; instead, some species rely on microbes living within their traps to produce the necessary enzymes required for prey digestion. Therefore, this study investigated the microbial community (bacteria and fungi) associated with Genlisea hispidula, a rare carnivorous species. Photosynthetic leaves, rhizophylls, and vesicles were processed after either being cleaned and rinsed in sterile water or after being surface sterilized. Tissues were ground in sterile water, serially diluted, lawn plated onto potato dextrose agar, and incubated in darkness for 24 h at 18–23 °C. Axenic cultures were obtained. Identity was determined via molecular sequence similarity of the full bacterial 16S rDNA gene or fungal ITS barcode regions. In total, 48 bacterial species and 29 fungal species were isolated, with Acidocella facilis and Burkholderia spp. being the most dominant isolated bacteria, and Trichomonascus vanleenenianus and Saitozyma spp. being the most dominant isolated fungi. Microbial diversity was greatest on photosynthetic leaves, while the vesicles had the lowest microbial diversity. This study is important because microbial communities play vital roles in maintaining host health and may be required when considering conservation.
Ultrasonographic Diagnosis of Finger Flexor Tendon Hypoplasia in a Child with Phalangeal Agenesis
Authors: Chen Cheng-I , Lin Hong-Yi , Wu Wei-Ting , Chang Ke-Vin , Özçakar Levent
Published: January 2024

Agenesis and hypoplasia affecting multiple flexor tendons within the same hand represent an exceedingly uncommon occurrence, with no previous studies addressing this condition. This report details a 4-year-old girl with agenesis of the right third and fourth fingers, who sought consultation due to the inability to flex her seemingly unaffected second and fifth fingers. Ultrasound examination revealed substantial thinning of the flexor tendons in the second to fifth digits, with a notable absence of attachment to the middle phalanx. In addition to flexor tendon hypoplasia, hypoplasia of the third and fourth middle phalanges was observed. Hand deformities featuring both finger agenesis and flexor tendon hypoplasia across multiple fingers were exceptionally rare. In such instances, ultrasound, in conjunction with radiography, emerges as the recommended initial imaging tool for comprehensive evaluation of both the phalangeal bones and flexor tendons.
Juvenile Primary Sjögren Syndrome in a 15-Year-Old Boy with Renal Involvement: A Case Report and Review of the Literature
Authors: Bouchalova Katerina , Flögelova Hana , Horak Pavel , Civrny Jakub , Mlcak Petr , Pink Richard , Michalek Jaroslav , Camborova Petra , Mikulkova Zuzana , Kriegova Eva
Published: January 2024

Juvenile primary Sjögren syndrome (pSS) with renal involvement is extremely rare, reported approximately in 50 children, predominantly girls. Here, we present the first reported case of a male child with juvenile pSS with ocular surface disease (previously keratoconjunctivitis sicca), submandibular salivary gland involvement, and tubulointerstitial nephritis. First, two symptoms were clinically apparent at presentation. We illustrate here that kidney involvement in pSS should be actively looked for, as juvenile pSS may be associated with asymptomatic renal involvement. Immunophenotyping of peripheral blood cells using multicolor flow cytometry revealed at the time of diagnosis changes in both adaptive (T memory cells and B memory cells), and innate immunity (an increased activation of natural killer cells, as well as monocytes and neutrophils, and an increased representation of intermediate monocytes). Our case report points to the importance of kidney examination, early diagnosis and therapy in juvenile pSS, as well as highlights international collaboration to obtain more data for this rare disease.
Transrectal Prostate Biopsy Approach in Men Undergoing Kidney Transplant: A Retrospective Cohort Study at Three Referral Academic Centers
Authors: Dell’Atti Lucio , Slyusar Viktoria , Ronchi Piero , Manno Stefano , Cambise Chiara
Published: January 2024

Background: Currently, there are no studies evaluating the feasibility of a prostate biopsy approach in men undergoing a kidney transplant (KT). Owing to this evidence, we planned a retrospective population-based study to evaluate our experience of a transrectal prostate biopsy (TR-PB) approach and studied the impact on the complication rate and outcomes in patients undergoing KT with suspected prostate cancer (PCa). Methods: We collected data from KT patients who underwent PB with a transrectal approach. One week and two weeks after the PB, patients’ information was collected regarding possible complications during the post-biopsy period. Results: A total of 121 patients were included in this study. Among them, Group 1 was composed of 59 patients undergoing TR-PB with an ultrasound (US) standard technique, and Group 2 consisted of 62 patients undergoing TR-PB with an MRI-US cognitive technique. We observed a 28.9% Clavien–Dindo grade ≤ 2 of early side effect rates (mostly rectal bleeding and other minor hematuria), with a very low rate of hospital re-admission for acute urinary retention (3.3%); only one man required hospitalization for rectal bleeding, and there were no major complications. Conclusions: We can affirm that TR-PB can be a safe procedure with a low risk of severe complications when performed by skilled specialists with a standardized procedural pathway.
Teaching in Higher Education after COVID-19: Optimizing Faculty Time and Effort Using a Proposed Model
Authors: Bajwa Naeem , Tudor Thomas , Varela Otmar , Leonard Karen
Published: January 2024

We propose a model for use in higher education after COVID-19 that addresses how to organize class methods for effective and efficient teaching outcomes for both students and instructors. Faculty have a finite amount of time that they may spend on their courses and must determine which time combinations produce the best outcomes. First, we discuss the key work-related challenges faced by faculty and contend that competing demands on faculty time result in inefficient allocation of their time and effort. We then model the issue as an optimization problem and illustrate through examples how to help faculty choose optimal method combinations along with time spent. We use time as a measure of effort and define which combination of methods might be most effective for achieving course learning objectives. There are opportunities for wider use of this methodology, as effective application of effort toward meeting the appropriate learning objectives should create better outcomes. Numerical examples are used to demonstrate the applicability of the proposed model, including how administrators can use it to support and encourage faculty.
Navigating New Normals: Student Perceptions, Experiences, and Mental Health Service Utilization in Post-Pandemic Academia
Authors: Galadima Hadiza , Dumadag Anne , Tonn Cara
Published: January 2024

This study explores the profound impacts of the COVID-19 pandemic on higher education, focusing on shifts in learning experiences and students’ intentions to utilize mental health services post-pandemic. Utilizing Andersen’s Behavioral Model of Health Services Use, this study assesses perceptions from a stratified random sample of college students on post-pandemic learning experiences and mental health service utilization intentions. Findings reveal a positive reception to university initiatives and a preference for ongoing virtual classes. There is an evident increase in, and varying intentions for, using mental health services, shaped by demographics, employment, and prior service utilization. Younger and female students showed an increased propensity to seek such services. This research underscores the urgent need for universities to bolster support for mental health and create awareness, alongside accommodating diverse learning preferences in transitioning back to traditional learning post-pandemic.
Utilization of H2O/CuO and Syltherm 800/CuO Nanofluids in a Concentrating Solar Collector with Photovoltaic Elements
Authors: Papingiotis Theodoros , Korres Dimitrios N. , Koronaki Irene , Tzivanidis Christos
Published: January 2024

This study examined the performance of a concentrating solar collector with an asymmetric reflector. Two receivers were investigated, differing in the presence of photovoltaic cells. The first one was equipped with cells on both sides while the other was without cells. The analysis was performed using a numerical model that integrates a combination of three-dimensional optical and thermal analyses developed in COMSOL. The investigation included studying the influence of CuO/water and CuO/Syltherm 800 nanofluids on the thermal performance for the receiver without photovoltaic elements, as well as on both thermal and electrical efficiencies for the hybrid receiver. Two volumetric concentrations of CuO in water and Syltherm 800, 3% and 5%, were explored with varying inlet temperatures, ranging from 20 °C to 80 °C for the hybrid solar unit and from 20 °C to 140 °C for the thermal solar unit. The outcomes of the examination were compared between the nanofluids and the pure base fluid. Properly pressurized water was considered in the case without photovoltaic elements.
Non-Invasive Method-Based Estimation of Battery State-of-Health with Dynamical Response Characteristics of Load Surges
Authors: Fan Yuhang , Lin Qiongbin , Huang Ruochen
Published: January 2024

Battery state-of-health (SOH) estimation is an effective approach to evaluate battery reliability and reduce maintenance costs for battery-based backup power supply systems. This paper proposes a novel SOH estimation method for batteries, which only uses the response characteristics of load surges and is, therefore, non-destructive to the estimated battery and its system. The discrete wavelet transform (DWT) method based on multi-resolution analysis (MRA) is used for wavelet energy features extraction, and the fuzzy cerebellar model neural network (FCMNN) is introduced to design the battery SOH estimator. The response voltage signals to load surges are used in the training and detection process of the FCMNN. Compared to conventional methods, the proposed method only exploits characteristics of online response signals to the inrush currents rather than injecting interference signals into the battery. The effectiveness of the proposed method is validated by detailed simulation analysis and experiments.
A Review of Thermal Energy Management of Diesel Exhaust after-Treatment Systems Technology and Efficiency Enhancement Approaches
Authors: Wu Gang , Feng Guoda , Li Yuelin , Ling Tao , Peng Xuejun , Su Zhilai , Zhao Xiaohuan
Published: January 2024

The DOC (diesel oxidation catalyst), DPF (diesel particulate filter), SCR (selective catalytic reduction), and ASC (ammonia slip catalyst) are widely used in diesel exhaust after-treatment systems. The thermal management of after-treatment systems using DOC, DPF, SCR, and ASC were investigated to improve the efficiency of these devices. This paper aims to identify the challenges of this topic and seek novel methods to control the temperature. Insulation methods and catalysts decrease the energy required for thermal management, which improves the efficiency of thermal management. Thermal insulation decreases the heat loss of the exhaust gas, which can reduce the after-treatment light-off time. The DOC light-off time was reduced by 75% under adiabatic conditions. A 400 W microwave can heat the DPF to the soot oxidation temperature of 873 K at a regeneration time of 150 s. An SCR burner can decrease NOx emissions by 93.5%. Electrically heated catalysts can decrease CO, HC, and NOx emissions by 80%, 80%, and 66%, respectively. Phase-change materials can control the SCR temperature with a two-thirds reduction in NOx emissions. Pt-Pd application in the catalyst can decrease the CO light-off temperature to 113 °C. Approaches of catalysts can enhance the efficiency of the after-treatment systems and reduce the energy consumption of thermal management.
Vegetation Effects on Phosphorus Runoff from Headwater Catchments in a Cool-Temperate Region with Landslides, Northern Japan
Authors: Ide Jun’ichiro , Naito Rikuto , Arata Yohei , Hirokawa Ryoma , Endo Izuki , Gomi Takashi
Published: January 2024

Forest vegetation and soils in headwaters can control runoff and surface erosion. However, it remains unclear how vegetation affects nutrient exports from cool-temperate forest headwaters during intense rain events that transport sediment-associated nutrients, such as phosphorus (P). To clarify this, we targeted an upstream landslide area and analyzed P contents in surface soils and total P (TP) in stream water of the undisturbed (UF) and landslide-bearing forest (LB) catchments. The soil P content was higher in the UF catchment than in the LB catchment, but differences in the average TP concentration and load during low flows between these catchments were not significant. Conversely, the overall runoff and the TP load were three and ten times higher in the LB catchment than in the UF catchment, respectively, during a rain event with daily precipitation of 49 mm, despite the soil P content being much lower in the LB catchment. Particulate P (PP) accounted for more than 90% of the TP load during the rain event in the LB catchment, whereas dissolved P accounted for more than 80% of the TP load in the UF catchment. Therefore, soil surface mobility strongly affected P transport in the forest catchments. Our study suggests that vegetation not only reduces PP loads by controlling runoff, but also influences stream P forms in cool-temperate forests.
Forest-Cover Changes in European Natura 2000 Sites in the Period 2012–2018
Authors: Santoro Antonio , Piras Francesco , Fiore Beatrice , Bazzurro Alessandra , Agnoletti Mauro
Published: January 2024

Protected areas have a key role in preserving biodiversity at different scales, as well as in providing ecosystem services to rural communities. Natura 2000 is the primary conservation network at the EU level, with the aim of protecting the most valuable species and habitats; it covers around 18.6% of the EU’s land area. The aim of this study is to assess the evolution of forest cover in EU Natura 2000 sites in the period 2012–2018 through GIS-based spatial analyses of the High-Resolution Layers produced in the framework of the Copernicus initiative. In 2018, fifteen EU countries had more than 50% of their surface covered by forests, with the top three countries being Slovenia (71.9%), the Czech Republic (70.5%), and Slovakia (69.3%). In 2012–2018, the net forest cover increase in EU Natura 2000 areas was equal to 105,750 ha/year (+1.7%). France, Bulgaria, and Germany recorded the greater net forest cover increase: 303,000 ha, 267,000 ha, and 150,000, respectively. France also recorded the highest yearly rate of forest gain (+51,491 ha/year). Most of the forest gain in EU Natura 2000 areas was found to be located between 0 and 200 m a.s.l. The study demonstrated that forest cover in EU Natura 2000 areas is increasing, with a consequent reduction of open spaces, homogenization of rural landscapes, and loss of landscape-scale biodiversity. The management and design of EU protected areas should consider the importance of preserving biodiversity-friendly land uses and practices, instead of promoting a diffuse “rewilding” with negative consequences for the landscape complexity and heterogeneity, as well as for biodiversity.
Terahertz Spectroscopic Identification of Roast Degree and Variety of Coffee Beans
Authors: Huang Luelue , Liu Miaoling , Li Bin , Chitrakar Bimal , Duan Xu
Published: January 2024

In this study, terahertz time-domain spectroscopy (THz-TDS) was proposed to identify coffee of three different varieties and three different roasting degrees of one variety. Principal component analysis (PCA) was applied to extract features from frequency-domain spectral data, and the extracted features were used for classification prediction through linear discrimination (LD), support vector machine (SVM), naive Bayes (NB), and k-nearest neighbors (KNN). The classification effect and misclassification of the model were analyzed via confusion matrix. The coffee varieties, namely Catimor, Typica 1, and Typica 2, under the condition of shallow drying were used for comparative tests. The LD classification model combined with PCA had the best effect of dimension reduction classification, while the speed and accuracy reached 20 ms and 100%, respectively. The LD model was found with the highest speed (25 ms) and accuracy (100%) by comparing the classification results of Typica 1 for three different roasting degrees. The coffee bean quality detection method based on THz-TDS combined with a modeling analysis method had a higher accuracy, faster speed, and simpler operation, and it is expected to become an effective detection method in coffee identification.
GC-MS, GC-IMS, and E-Nose Analysis of Volatile Aroma Compounds in Wet-Marinated Fermented Golden Pomfret Prepared Using Different Cooking Methods
Authors: Chen Qiuhan , Yang Xuebo , Hong Pengzhi , Liu Meijiao , Li Zhuyi , Zhou Chunxia , Zhong Saiyi , Liu Shouchun
Published: January 2024

The cooking method is extremely important for the production of low-salt, wet-marinated, fermented golden pomfret because it strongly influences its flavor components and organoleptic quality. There are also significant differences in flavor preferences in different populations. The present study analyzed differences in the aroma characteristics of wet-marinated fermented golden pomfret after boiling, steaming, microwaving, air-frying, and baking using a combination of an electronic nose, GC-IMS, and SPME-GC-MS. Electronic nose PCA showed that the flavors of the boiled (A), steamed (B), and microwaved (C) treatment groups were similar, and the flavors of the baking (D) and air-frying (E) groups were similar. A total of 72 flavor compounds were detected in the GC-IMS analysis, and the comparative analysis of the cooked wet-marinated and fermented golden pomfret yielded a greater abundance of flavor compounds. SPME-GC-MS analysis detected 108 flavor compounds, and the results were similar for baking and air-frying. Twelve key flavor substances, including hexanal, isovaleraldehyde, and (E)-2-dodecenal, were identified by orthogonal partial least-squares discriminant analysis (OPLS-DA) and VIP analysis. These results showed that the cooking method could be a key factor in the flavor distribution of wet-marinated fermented golden pomfret, and consumers can choose the appropriate cooking method accordingly. The results can provide theoretical guidance for the more effective processing of fish products and the development of subsequent food products.
Effects of Parboiling on Chemical Properties, Phenolic Content and Antioxidant Capacity in Colored Landrace Rice
Authors: Pinta Wanwipa , Aninbon Chorkaew , Kaewtaphan Phissanu , Kunyanee Kannika
Published: January 2024

Parboiling influences chemical compositions in rice grains. The objectives of this study were to evaluate the change in chemical content, total phenolic content and antioxidant capacity of landrace rice genotypes under parboiling conditions and to identify the genotypes suitable for production of parboiled rice. Landrace rice varieties used in this study consisted of Glam Feang, Glam Tonkeaw, Kawgum, Glam Luem Phua, Medmakham, Deang Sakonnakhon, Sang Yod, Kawniewd-eang, Mali Deang, KDML105 and RD6. Parboiling reduced fiber content, total phenolic content and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity in rice grains. Fiber contents were 1.46% in brown rice (unpolished rice) and 1.40% in parboiled rice (24 h of soaking and 48 h of incubation). Total phenolic contents were 205.67 mg/100 g seed in brown rice and 35.34 mg/100 g seed in parboiled rice. Antioxidant capacity (DPPH) reduced from 68.45% in brown rice to 26.23% in parboiled rice. Ash content and protein content were not significantly affected by the parboiling process. Medmakham cv. had the highest total phenolic content and antioxidant capacity in brown rice and parboiled rice. Gum Leamphea cv. and Medmakham cv. were the best genotypes for ash content, protein content, total phenolic content and antioxidant capacity (DPPH) in brown rice and parboiled rice. Glam Feang cv. had the highest protein contents in brown rice and parboiled rice although it had low total phenolic content and antioxidant capacity. Cluster analysis further showed variation among genotypes, revealing distinct groupings in brown rice and parboiled rice based on chemical properties, phenolic content and antioxidant capacity. This research significantly contributes to a better understanding on how parboiling affects rice compositions and nutritional values. It emphasizes the importance of nuanced comprehension of how different rice varieties respond to parboiling, aiding informed decisions in rice processing and selection to meet specific nutritional needs.
A Self-Healing Gel with an Organic–Inorganic Network Structure for Mitigating Circulation Loss
Authors: Wang Cheng , Sun Jinsheng , Long Yifu , Huang Hongjun , Song Juye , Wang Ren , Qu Yuanzhi , Yang Zexing
Published: January 2024

Lost circulation control remains a challenge in drilling operations. Self-healing gels, capable of self-healing in fractures and forming entire gel block, exhibit excellent resilience and erosion resistance, thus finding extensive studies in lost circulation control. In this study, layered double hydroxide, Acrylic acid, 2-Acrylamido-2-methylpropane sulfonic acid, and CaCl2 were employed to synthesize organic-inorganic nanocomposite gel with self-healing properties. The chemical properties of nanocomposite gels were characterized using X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscope, X-ray photoelectron spectroscopy and thermogravimetric analysis. layered double hydroxide could be dispersed and exfoliated in the mixed solution of Acrylic acid and 2-Acrylamido-2-methylpropane sulfonic acid, and the swelling behavior, self-healing time, rheological properties, and mechanical performance of the nanocomposite gels were influenced by the addition of layered double hydroxide and Ca2+. Optimized nanocomposite gel AC6L3, at 90 °C, exhibits only a self-healing time of 3.5 h in bentonite mud, with a storage modulus of 4176 Pa, tensile strength of 6.02 kPa, and adhesive strength of 1.94 kPa. In comparison to conventional gel, the nanocomposite gel with self-healing capabilities demonstrated superior pressure-bearing capacity. Based on these characteristics, the nanocomposite gel proposed in this work hold promise as a candidate lost circulation material.
Ionic Strength Impacts the Physical Properties of Agarose Hydrogels
Authors: Sacco Pasquale , Piazza Francesco , Marsich Eleonora , Abrami Michela , Grassi Mario , Donati Ivan
Published: January 2024

Agarose is a natural polysaccharide known for its ability to form thermoreversible hydrogels. While the effects of curing temperature and polysaccharide concentration on mechanical properties have been discussed in the literature, the role of ionic strength has been less studied. In the present manuscript, we investigate the effects of supporting salt concentration and the role of cation (i.e. Na+ or Li+, neighbors in the Hofmeister series), on the setting and performance of agarose hydrogels. Compressive and rheological measurements show that the supporting salts reduce the immediate elastic response of agarose hydrogels, with Li+ showing a stronger effect than Na+ at high ionic strength, while they significantly increase the extent of linear stress-strain response (i.e., linear elasticity). The presence of increasing amounts of added supporting salt also leads to a reduction in hysteresis during mechanical deformation due to loading and unloading cycles, which is more pronounced with Li+ than with Na+. The combination of rheological measurements and NMR relaxometry shows a mesh size in agarose hydrogels in the order of 6–17 nm, with a thickness of the water layer bound to the biopolymer of about 3 nm. Of note, the different structuring of the water within the hydrogel network due to the different alkali seems to play a role for the final performance of the hydrogels.
X Chromosome-Specific Repeats in Non-Domestic Bovidae
Authors: Kubickova Svatava , Kopecna Olga , Cernohorska Halina , Rubes Jiri , Vozdova Miluse
Published: January 2024

Repetitive sequences form a substantial and still enigmatic part of the mammalian genome. We isolated repetitive DNA blocks of the X chromosomes of three species of the family Bovidae: Kobus defassa (KDEXr sequence), Bos taurus (BTAXr sequence) and Antilope cervicapra (ACEXr sequence). The copy numbers of the isolated sequences were assessed using qPCR, and their chromosomal localisations were analysed using FISH in ten bovid tribes and in outgroup species. Besides their localisation on the X chromosome, their presence was also revealed on the Y chromosome and autosomes in several species. The KDEXr sequence abundant in most Bovidae species also occurs in distant taxa (Perissodactyla and Carnivora) and seems to be evolutionarily older than BTAXr and ACEXr. The ACEXr sequence, visible only in several Antilopini species using FISH, is probably the youngest, and arised in an ancestor common to Bovidae and Cervidae. All three repetitive sequences analysed in this study are interspersed among gene-rich regions on the X chromosomes, apparently preventing the crossing-over in their close vicinity. This study demonstrates that repetitive sequences on the X chromosomes have undergone a fast evolution, and their variation among related species can be beneficial for evolutionary studies.
Clearing the Air: Understanding the Impact of Wildfire Smoke on Asthma and COPD
Authors: Wilgus May-Lin , Merchant Maryum
Published: January 2024

Wildfires are a global natural phenomenon. In North America, wildfires have not only become more frequent, but also more severe and longer in duration, a trend ascribed to climate change combined with large fuel stores left from modern fire suppression. The intensification of wildfire activity has significant implications for planetary health and public health, as exposure to fine particulate matter (PM2.5) in wildfire smoke is linked to adverse health effects. This review focuses on respiratory morbidity from wildfire smoke exposure. Inhalation of wildfire PM2.5 causes lung injury via oxidative stress, local and systemic inflammation, airway epithelium compromise, and increased vulnerability to infection. Wildfire PM2.5 exposure results in exacerbations of pre-existing asthma and chronic obstructive pulmonary disease, with an escalation in healthcare utilization, including emergency department visits and hospitalizations. Wildfire smoke exposure may be associated with asthma onset, long-term impairment of lung function, and increased all-cause mortality. Children, older adults, occupationally-exposed groups, and possibly women are the most at risk from wildfire smoke. Future research is needed to clarify best practices for risk mitigation and wildfire management.
The Effects of Equine-Assisted Learning on Adolescents with Internet Gaming Disorder
Authors: Park Hyoungjin , Jung Taewoon
Published: January 2024

During the COVID-19 pandemic, internet gaming became more popular as a way to cope with stress, but excessive gaming can lead to mental health issues like internet gaming disorder (IGD). IGD has serious consequences, especially among children and young adults, and the gaming industry’s profits continue to grow. This study aims to understand the mental and behavioral health of adolescents with IGD participating in an equine-assisted learning (EAL) program and assess the changes in their addiction tendencies and emotional and behavioral problems. The results showed that the participants’ tendency towards internet gaming addiction and emotional and behavioral problems decreased immediately after the EAL program, but they rose again a month later. This suggests the importance of ongoing program involvement. As COVID-19 restrictions ease worldwide, this study highlights the increasing risk of IGD. It suggests that EAL could be a valuable approach to treating behavioral addictions, including gaming addiction. While prior research has shown the effectiveness of EAL in treating substance addiction, more research is needed to explore its potential in treating various types of addictions, such as gambling or gaming addictions.
Responsiveness of Isokinetic Dynamometry in Patients with Osteoarthritis after Knee and Hip Arthroplasty: A Prospective Repeated-Measures Cohort Study
Authors: Prüfer Ferdinand , Pavlović Monika , Matko Špela , Löfler Stefan , Fischer Michael J. , Šarabon Nejc , Grote Vincent
Published: January 2024

Functional assessments are crucial for the evaluation of rehabilitation after total knee (TKA) and hip (THA) arthroplasty. Muscle strength, a key determinant of physical function (PF), is often measured with isokinetic dynamometry (ID), which is considered the gold standard. However, studies lack evaluations of responsiveness—the ability to detect changes over time. This study aims to determine the responsiveness of ID in measuring PF in TKA and THA rehabilitation—is muscle strength a valid indicator for assessing improvement in rehabilitation processes? The pre- and post-surgery PF of 20 osteoarthritis patients (age 55–82) was assessed, using ID, performance-based and self-reported measures. Responsiveness was evaluated by comparing the observed relationship of changes in ID and PF scores with the a priori defined expected relationship of change scores. While the performance-based and self-reported measures showed significant improvements post-surgery (Cohen’s d [0.42, 1.05] p < 0.05), ID showed no significant differences. Moderate correlations were found between changes in some ID parameters and selected functional tests (r ≈|0.5|, p < 0.05). Responsiveness was solely found for the peak torque of knee extension at 180°/s on the operated side. Responsiveness is an often-overlooked psychometric property of outcome measurements. The findings suggest that ID may not be fully responsive to the construct of PF after TKA and THA, raising questions about its role and usefulness in this context and the need for more appropriate assessment methods.