bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Engagement in Antenatal Health Behavior Programs—Experiences of Women Who Did Not Attend a Healthy Lifestyle Telephone Coaching Program
Authors: Fry Jessica , Wilkinson Shelley A. , Willcox Jane , Henny Michaela , McGuire Lisa , Guthrie Taylor M. , Meloncelli Nina , de Jersey Susan
Published: April 2023

Living Well during Pregnancy (LWdP) is a telephone-based antenatal health behavior intervention that has been shown to improve healthy eating behaviors and physical activity levels during pregnancy. However, one-third of eligible, referred women did not engage with or dropped out of the service. This study aimed to explore the experiences and perceptions of women who were referred but did not attend or complete the LWdP program to inform service improvements and adaptations required for scale and spread and improve the delivery of patient-centered antenatal care. Semi-structured telephone interviews were conducted with women who attended ≤2 LWdP appointments after referral. The interviews were thematically analyzed and mapped to the Theoretical Domains Framework and Behavior Change Wheel/COM-B Model to identify the barriers and enablers of program attendance and determine evidence-based interventions needed to improve service engagement and patient-centered antenatal care. Three key themes were identified: (1) the program content not meeting women’s expectations and goals; (2) the need for flexible, multimodal healthcare; and (3) information sharing throughout antenatal care not meeting women’s information needs. Interventions to improve women’s engagement with LWdP and patient-centered antenatal care were categorized as (1) adaptations to LWdP, (2) training and support for program dietitians and antenatal healthcare professionals, and (3) increased promotion of positive health behaviors during pregnancy. Women require flexible and personalized delivery of the LWdP that is aligned with their individual goals and expectations. The use of digital technology has the potential to provide flexible, on-demand access to and engagement with the LWdP program, healthcare professionals, and reliable health information. All healthcare professionals are vital to the promotion of positive health behaviors in pregnancy, with the ongoing training and support necessary to maintain clinician confidence and knowledge of healthy eating, physical activity, and weight gain during pregnancy.
Nutritional Management of Patients with Head and Neck Cancer—A Comprehensive Review
Authors: Martinovic Dinko , Tokic Daria , Puizina Mladinic Ema , Usljebrka Mislav , Kadic Sanja , Lesin Antonella , Vilovic Marino , Lupi-Ferandin Slaven , Ercegovic Sasa , Kumric Marko , Bukic Josipa , Bozic Josko
Published: April 2023

While surgical therapy for head and neck cancer (HNC) is showing improvement with the advancement of reconstruction techniques, the focus in these patients should also be shifting to supportive pre and aftercare. Due to the highly sensitive and anatomically complex region, these patients tend to exhibit malnutrition, which has a substantial impact on their recovery and quality of life. The complications and symptoms of both the disease and the therapy usually make these patients unable to orally intake food, hence, a strategy should be prepared for their nutritional management. Even though there are several possible nutritional modalities that can be administrated, these patients commonly have a functional gastrointestinal tract, and enteral nutrition is indicated over the parenteral option. However, after extensive research of the available literature, it seems that there is a limited number of studies that focus on this important issue. Furthermore, there are no recommendations or guidelines regarding the nutritional management of HNC patients, pre- or post-operatively. Henceforth, this narrative review summarizes the nutritional challenges and management modalities in this particular group of patients. Nonetheless, this issue should be addressed in future studies and an algorithm should be established for better nutritional care of these patients.
Exploration of Metabolic Biomarkers Linking Red Meat Consumption to Ischemic Heart Disease Mortality in the UK Biobank
Authors: Fan Bohan , Huang Xin , Zhao Jie
Published: April 2023

Growing evidence suggests that red meat consumption is a risk factor for cardiovascular health, with potential sex disparity. The metabolic mechanisms have not been fully understood. Using the UK Biobank, first we examined the associations of unprocessed red meat and processed meat with ischemic heart disease (IHD) mortality overall and by sex using logistic regression. Then, we examined the overall and sex-specific associations of red meat consumption with metabolites using multivariable regression, as well as the associations of selected metabolites with IHD mortality using logistic regression. We further selected metabolic biomarkers that are linked to both red meat consumption and IHD, with concordant directions. Unprocessed red meat and processed meat consumption was associated with higher IHD mortality overall and in men. Thirteen metabolites were associated with both unprocessed red meat and IHD mortality overall and showed a consistent direction, including triglycerides in different lipoproteins, phospholipids in very small very-low-density lipoprotein (VLDL), docosahexaenoic acid, tyrosine, creatinine, glucose, and glycoprotein acetyls. Ten metabolites related to triglycerides and VLDL were positively associated with both unprocessed red meat consumption and IHD mortality in men, but not in women. Processed meat consumption showed similar results with unprocessed red meat. Triglycerides in lipoproteins, fatty acids, and some nonlipid metabolites may play a role linking meat consumption to IHD. Triglycerides and VLDL-related lipid metabolism may contribute to the sex-specific associations. Sex differences should be considered in dietary recommendations.
Comparison of Three Low-Molecular-Weight Fluorescent Probes for Measuring Free Zinc Levels in Cultured Mammary Cells
Authors: Hübner Christopher , Keil Claudia , Jürgensen Anton , Barthel Lars , Haase Hajo
Published: April 2023

Free zinc is a critical regulator in signal transduction and affects many cellular processes relevant to cancer, including proliferation and cell death. Acting as a second messenger, altered free intracellular zinc has fundamental effects on regulating enzymes such as phosphatases and caspases. Therefore, the determination of free intracellular zinc levels is essential to assess its influence on the signaling processes involved in cancer development and progression. In this study, we compare three low-molecular-weight fluorescent probes, ZinPyr-1, TSQ, and FluoZin-3, for measuring free zinc in different mammary cell lines (MCF10A, MCF7, T47D, and MDA-MB-231). In summary, ZinPyr-1 is the most suitable probe for free Zn quantification. It responds well to calibration based on minimal fluorescence in the presence of the chelator TPEN (N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine) and maximal fluorescence by saturation with ZnSO4, resulting in the detection of free intracellular zinc in breast cancer subtypes ranging from 0.62 nM to 1.25 nM. It also allows for measuring the zinc fluxes resulting from incubation with extracellular zinc, showing differences in the zinc uptake between the non-malignant MCF10A cell line and the other cell lines. Finally, ZinPyr-1 enables the monitoring of sub-cellular distributions by fluorescence microscopy. Altogether, these properties provide a basis for the further exploration of free zinc in order to realize its full potential as a possible biomarker or even therapeutic target in breast cancer.
Differences in Gut Microbiome Profile between Healthy Children and Children with Inflammatory Bowel Disease and/or Autoimmune Liver Disease: A Case-Control Study
Authors: Lopez Robert N. , Leach Steven T. , Bowcock Nerissa , Coker Elise , Shapiro Amanda J. , Day Andrew S. , Lemberg Daniel A.
Published: April 2023

Background: The role of gastrointestinal microbiome in health and disease is increasingly appreciated. A significant amount of evidence clearly points to a dysbiosis manifest in inflammatory bowel disease (IBD) when compared to healthy controls. Less understood is the microbiome profile in autoimmune liver disease (AILD). Both adult and paediatric data indicate a distinct microbial signature in patients with IBD and co-existent primary sclerosing cholangitis (PSC), which is unique and different compared to the microbial signature that exists in patients with IBD alone. However, there is limited information on the microbiome make-up of patients with parenchymal liver disease, with or without IBD. Methods: The present study sought to compare the microbiome of children with IBD, to those with IBD-AILD, those with AILD alone and those of healthy controls. Results: Results from this work indicate that children with AILD have a microbiome profile that mirrors healthy controls. Conclusion: Those with IBD-AILD and IBD have similar microbiome profiles which are distinct from AILD alone and healthy controls. This suggests that the dysbiosis in these groups is primarily due to IBD rather than AILD.
Timing of Symptoms of Early-Onset Sepsis after Intrapartum Antibiotic Prophylaxis: Can It Inform the Neonatal Management?
Authors: Berardi Alberto , Trevisani Viola , Di Caprio Antonella , Caccamo Paola , Latorre Giuseppe , Loprieno Sabrina , Foglianese Alessandra , Laforgia Nicola , Perrone Barbara , Nicolini Giangiacomo , Ciccia Matilde , Capretti Maria Grazia , Giugno Chiara , Rizzo Vittoria , Merazzi Daniele , Fanaro Silvia , Taurino Lucia , Pulvirenti Rita Maria , Orlandini Silvia , Auriti Cinzia , Haass Cristina , Ligi Laura , Vellani Giulia , Tzialla Chryssoula , Tuoni Cristina , Santori Daniele , Baroni Lorenza , China Mariachiara , Bua Jenny , Visintini Federica , Decembrino Lidia , Creti Roberta , Miselli Francesca , Bedetti Luca , Lugli Licia
Published: April 2023

The effectiveness of “inadequate” intrapartum antibiotic prophylaxis (IAP administered < 4 h prior to delivery) in preventing early-onset sepsis (EOS) is debated. Italian prospective surveillance cohort data (2003–2022) were used to study the type and duration of IAP according to the timing of symptoms onset of group B streptococcus (GBS) and E. coli culture-confirmed EOS cases. IAP was defined “active” when the pathogen yielded in cultures was susceptible. We identified 263 EOS cases (GBS = 191; E. coli = 72). Among GBS EOS, 25% had received IAP (always active when beta-lactams were administered). Most IAP-exposed neonates with GBS were symptomatic at birth (67%) or remained asymptomatic (25%), regardless of IAP duration. Among E. coli EOS, 60% were IAP-exposed. However, IAP was active in only 8% of cases, and these newborns remained asymptomatic or presented with symptoms prior to 6 h of life. In contrast, most newborns exposed to an “inactive” IAP (52%) developed symptoms from 1 to >48 h of life. The key element to define IAP “adequate” seems the pathogen’s antimicrobial susceptibility rather than its duration. Newborns exposed to an active antimicrobial (as frequently occurs with GBS infections), who remain asymptomatic in the first 6 h of life, are likely uninfected. Because E. coli isolates are often unsusceptible to beta-lactam antibiotics, IAP-exposed neonates frequently develop symptoms of EOS after birth, up to 48 h of life and beyond.
Mechanochemical Properties of Mucoadhesive Tablets Based on PVP/HPβCD Electrospun Nanofibers as Local Delivery of Polygoni cuspidati Extract for Treating Oral Infections
Authors: Paczkowska-Walendowska Magdalena , Szymanowska Daria , Cielecka-Piontek Judyta
Published: April 2023

This study investigated the ability of PVP/HPβCD-based electrospun nanofibers to enhance the dissolution rate of poorly soluble polydatin and resveratrol, the main active components of Polygoni cuspidati extract. To make a solid unit dosage form that would be easier to administer, extract-loaded nanofibers were ground. SEM examination was used to analyze the nanostructure of the fibers, and the results of the cross-section of the tablets showed that they had maintained their fibrous structure. The release of the active compounds (polydatin and resveratrol) in the mucoadhesive tablets was complete and prolonged in time. Additionally, the possibility of staying on the mucosa for a prolonged time has also been proven for both tablets from PVP/HPβCD-based nanofibers and powder. The appropriate physicochemical properties of the tablets, along with the proven antioxidant, anti-inflammatory, and antibacterial properties of P. cuspidati extract, highlight the particular benefits of the mucoadhesive formulation for use as a drug delivery system for periodontal diseases.
Microorganism-Derived Molecules as Enzyme Inhibitors to Target Alzheimer’s Diseases Pathways
Authors: Nguyen Thi Hanh , Wang San-Lang , Nguyen Van Bon
Published: April 2023

Alzheimer’s disease (AD) is the most common form of dementia. It increases the risk of other serious diseases and causes a huge impact on individuals, families, and socioeconomics. AD is a complex multifactorial disease, and current pharmacological therapies are largely based on the inhibition of enzymes involved in the pathogenesis of AD. Natural enzyme inhibitors are the potential sources for targeting AD treatment and are mainly collected from plants, marine organisms, or microorganisms. In particular, microbial sources have many advantages compared to other sources. While several reviews on AD have been reported, most of these previous reviews focused on presenting and discussing the general theory of AD or overviewing enzyme inhibitors from various sources, such as chemical synthesis, plants, and marine organisms, while only a few reviews regarding microbial sources of enzyme inhibitors against AD are available. Currently, multi-targeted drug investigation is a new trend for the potential treatment of AD. However, there is no review that has comprehensively discussed the various kinds of enzyme inhibitors from the microbial source. This review extensively addresses the above-mentioned aspect and simultaneously updates and provides a more comprehensive view of the enzyme targets involved in the pathogenesis of AD. The emerging trend of using in silico studies to discover drugs concerning AD inhibitors from microorganisms and perspectives for further experimental studies are also covered here.
Chrysin Is Immunomodulatory and Anti-Inflammatory against Complete Freund’s Adjuvant-Induced Arthritis in a Pre-Clinical Rodent Model
Authors: Faheem Muhammad Asif , Akhtar Tasleem , Naseem Nadia , Aftab Usman , Zafar Muhammad Shoaib , Hussain Safdar , Shahzad Muhammad , Gobe Glenda Carolyn
Published: April 2023

Chrysin (5,7-dihydroxyflavone) has many pharmacological properties including anti-inflammatory actions. The objective of this study was to evaluate the anti-arthritic activity of chrysin and to compare its effect with the non-steroidal anti-inflammatory agent, piroxicam, against complete Freund’s adjuvant (CFA)-induced arthritis in a pre-clinical model in rats. Rheumatoid arthritis was induced by injecting CFA intra-dermally in the sub-plantar region of the left hind paw of rats. Chrysin (50 and 100 mg/kg) and piroxicam (10 mg/kg) were given to rats with established arthritis. The model of arthritis was characterized using an index of arthritis, with hematological, biological, molecular, and histopathological parameters. Treatment with chrysin significantly reduced the arthritis score, inflammatory cells, erythrocyte sedimentation rate, and rheumatoid factor. Chrysin also reduced the mRNA levels of tumor necrosis factor, nuclear factor kappa-B, and toll-like recepter-2 and increased anti-inflammatory cytokines interleukin-4 and -10, as well as the hemoglobin levels. Using histopathology and microscopy, chrysin reduced the severity of arthritis in joints, infiltration of inflammatory cells, subcutaneous inflammation, cartilage erosion, bone erosion, and pannus formation. Chrysin showed comparable effects to piroxicam, which is used for the treatment of rheumatoid arthritis. The results showed that chrysin possesses anti-inflammatory and immunomodulatory effects that make it a potential drug for the treatment of arthritis.
Design, Development, and Evaluation of Treprostinil Embedded Adhesive Transdermal Patch
Authors: Alissa Ibrahim , Nair Anroop B. , Aldhubiab Bandar , Shah Hiral , Shah Jigar , Mewada Vivek , Almuqbil Rashed M. , Jacob Shery
Published: April 2023

Clinical application of treprostinil in pulmonary arterial hypertension is hampered by adverse effects caused by its high dosing frequency. The objective of this investigation was to Formulate an adhesive-type transdermal patch of treprostinil and evaluate it both in vitro and in vivo. A 32-factorial design was utilized to optimize the selected independent variables (X1: drug amount, X2: enhancer concentration) on the response variables (Y1: drug release, Y2: transdermal flux). The optimized patch was evaluated for various pharmaceutical properties, skin irritation, and pharmacokinetics in rats. Optimization results signify considerable influence (p < 0.0001) of X1 on both Y1 and Y2, as compared to X2. The optimized patch possesses higher drug content (>95%), suitable surface morphology, and an absence of drug crystallization. FTIR analysis revealed compatibility of the drug with excipients, whereas DSC thermograms indicate that the drug exists as amorphous in the patch. The adhesive properties of the prepared patch confirm adequate adhesion and painless removal, while the skin irritation study confirms its safety. A steady drug release via Fickian diffusion and greater transdermal delivery (~23.26 µg/cm2/h) substantiate the potential of the optimized patch. Transdermal therapy resulted in higher treprostinil absorption (p < 0.0001) and relative bioavailability (237%) when compared to oral administration. Overall, the results indicate that the developed drug in the adhesive patch can effectively deliver treprostinil through the skin and could be a promising treatment option for pulmonary arterial hypertension.
Amorphous Pterostilbene Delivery Systems Preparation—Innovative Approach to Preparation Optimization
Authors: Rosiak Natalia , Tykarska Ewa , Cielecka-Piontek Judyta
Published: April 2023

The aim of our research was to improve the solubility and antioxidant activity of pterostilbene (PTR) by developing a novel amorphous solid dispersion (ASD) with Soluplus® (SOL). DSC analysis and mathematical models were used to select the three appropriate PTR and SOL weight ratios. The amorphization process was carried out by a low-cost and green approach involving dry milling. An XRPD analysis confirmed the full amorphization of systems in 1:2 and 1:5 weight ratios. One glass transition (Tg) observed in DSC thermograms confirmed the complete miscibility of the systems. The mathematical models indicated strong heteronuclear interactions. SEM micrographs suggest dispersed PTR within the SOL matrix and a lack of PTR crystallinity, and showed that after the amorphization process, PTR-SOL systems had a smaller particle size and larger surface area compared with PTR and SOL. An FT-IR analysis confirmed that hydrogen bonds were responsible for stabilizing the amorphous dispersion. HPLC studies showed no decomposition of PTR after the milling process. PTR’s apparent solubility and antioxidant activity after introduction into ASD increased compared to the pure compound. The amorphization process improved the apparent solubility by ~37-fold and ~28-fold for PTR-SOL, 1:2 and 1:5 w/w, respectively. The PTR-SOL 1:2 w/w system was preferred due to it having the best solubility and antioxidant activity (ABTS: IC50 of 56.389 ± 0.151 µg·mL−1 and CUPRAC: IC0.5 of 82.52 ± 0.88 µg·mL−1).
A Method for Electroporation of Cre Recombinase Protein into Intact Nicotiana tabacum Cells
Authors: Furuhata Yuichi , Egi Emiko , Murakami Tomi , Kato Yoshio
Published: April 2023

The Cre/lox recombination system has become a powerful technology for gene function analysis in a broad spectrum of cell types and organisms. In our previous report, Cre protein had been successfully delivered into intact Arabidopsis thaliana cells using electroporation. To expand the feasibility of the method of protein electroporation to other plant cells, here we attempt the protein electroporation into tobacco-derived BY-2 cells, one of the most frequently used plant cell lines for industrial production. In this study, we successfully deliver Cre protein into BY-2 cells with intact cell walls by electroporation with low toxicity. Targeted loxP sequences in the BY-2 genome are recombined significantly. These results provide useful information for genome engineering in diverse plant cells possessing various types of cell walls.
Water Stress and Seed Color Interacting to Impact Seed and Oil Yield, Protein, Mucilage, and Secoisolariciresinol Diglucoside Content in Cultivated Flax (Linum usitatissimum L.)
Authors: Zare Sara , Mirlohi Aghafakhr , Sabzalian Mohammad R. , Saeidi Ghodratollah , Koçak Mehmet Zeki , Hano Christophe
Published: April 2023

Flaxseed (Linum usitatissimum L.) is a plant with a wide range of medicinal, health, nutritional, and industrial uses. This study assessed the genetic potential of yellow and brown seeds in thirty F4 families under different water conditions concerning seed yield, oil, protein, fiber, mucilage, and lignans content. Water stress negatively affected seed and oil yield, while it positively affected mucilage, protein, lignans, and fiber content. The total mean comparison showed that under normal moisture conditions, seed yield (209.87 g/m2) and most quality traits, including oil (30.97%), secoisolariciresinol diglucoside (13.89 mg/g), amino acids such as arginine (1.17%) and histidine (1.95%), and mucilage (9.57 g/100 g) were higher in yellow-seeded genotypes than the brown ones ((188.78 g/m2), (30.10%), (11.66 mg/g), (0.62%), (1.87%), and (9.35 g/100 g), respectively). Under water stress conditions, brown-seeded genotypes had a higher amount of fiber (16.74%), seed yield (140.04 g/m2), protein (239.02 mg. g−1), methionine (5.04%), and secondary metabolites such as secoisolariciresinol diglucoside (17.09 mg/g), while their amounts in families with yellow seeds were 14.79%, 117.33 g/m2, 217.12 mg. g−1, 4.34%, and 13.98 mg/g, respectively. Based on the intended food goals, different seed color genotypes may be appropriate for cultivation under different moisture environments.
Analysis of the Relative Importance of Stand Structure and Site Conditions for the Productivity, Species Diversity, and Carbon Sequestration of Cunninghamia lanceolata and Phoebe bournei Mixed Forest
Authors: Wang Yiru , Liu Zhaohua , Tang Tao , Li Jiping
Published: April 2023

Forest stand structure (the characteristics and interrelationships of live trees) and site conditions (the physical and environmental characteristics of a specific location) have been linked to forest regeneration, nutrient cycling, wildlife habitat, and climate regulation. While the effects of stand structure (i.e., spatial and non-spatial) and site conditions on the single function of Cunninghamia lanceolata and Phoebe bournei (CLPB) mixed forest have been studied in previous studies, the relative importance of stand structure and site conditions in terms of productivity, species diversity, and carbon sequestration remains unresolved. In this study, a structural equation model (SEM) was adopted to analyze the relative importance of stand structure and site conditions for the forest productivity, species diversity, and carbon sequestration of CLPB mixed forest in Jindong Forestry in Hunan Province. Our research demonstrates that site conditions have a greater influence on forest functions than stand structure, and that non-spatial structures have a greater overall impact on forest functions than spatial structures. Specifically, the intensity of the influence of site conditions and non-spatial structure on functions is greatest for productivity, followed by carbon sequestration and species diversity. In contrast, the intensity of the influence of spatial structure on functions is greatest for carbon sequestration, followed by species diversity and productivity. These findings provide valuable insights for the management of CLPB mixed forest in Jindong Forestry and have significant reference value for the close-to-natural forest management (CTNFM) of pure Cunninghamia lanceolata forests.
Shrub Invasion Overrides the Effect of Imposed Drought on the Photosynthetic Capacity and Physiological Responses of Mediterranean Cork Oak Trees
Authors: Lobo-do-Vale Raquel , Rafael Teresa , Haberstroh Simon , Werner Christiane , Caldeira Maria Conceição
Published: April 2023

Mediterranean ecosystems face threats from both climate change and shrub invasion. As shrub cover increases, competition for water intensifies, exacerbating the negative effects of drought on ecosystem functioning. However, research into the combined effects of drought and shrub invasion on tree carbon assimilation has been limited. We used a Mediterranean cork oak (Quercus suber) woodland to investigate the effects of drought and shrub invasion by gum rockrose (Cistus ladanifer) on cork oak carbon assimilation and photosynthetic capacity. We established a factorial experiment of imposed drought (ambient and rain exclusion) and shrub invasion (invaded and non-invaded) and measured leaf water potential, stomatal conductance and photosynthesis as well as photosynthetic capacity in cork oak and gum rockrose over one year. We observed distinct detrimental effects of gum rockrose shrub invasion on the physiological responses of cork oak trees throughout the study period. Despite the imposed drought, the impact of shrub invasion was more pronounced, resulting in significant photosynthetic capacity reduction of 57% during summer. Stomatal and non-stomatal limitations were observed under moderate drought in both species. Our findings provide significant knowledge on the impact of gum rockrose invasion on the functioning of cork oak and can be used to improve the representation of photosynthesis in terrestrial biosphere models.
TM-Free and TM-Catalyzed Mechanosynthesis of Functional Polymers
Authors: Al-Ithawi Wahab , Khasanov Albert , Kovalev Igor , Nikonov Igor , Platonov Vadim , Kopchuk Dmitry , Santra Sougata , Zyryanov Grigory , Ranu Brindaban
Published: April 2023

Mechanochemically induced methods are commonly used for the depolymerization of polymers, including plastic and agricultural wastes. So far, these methods have rarely been used for polymer synthesis. Compared to conventional polymerization in solutions, mechanochemical polymerization offers numerous advantages such as less or no solvent consumption, the accessibility of novel structures, the inclusion of co-polymers and post-modified polymers, and, most importantly, the avoidance of problems posed by low monomer/oligomer solubility and fast precipitation during polymerization. Consequently, the development of new functional polymers and materials, including those based on mechanochemically synthesized polymers, has drawn much interest, particularly from the perspective of green chemistry. In this review, we tried to highlight the most representative examples of transition-metal (TM)-free and TM-catalyzed mechanosynthesis of some functional polymers, such as semiconductive polymers, porous polymeric materials, sensory materials, materials for photovoltaics, etc.
Self-Healing of Recombinant Spider Silk Gel and Coating
Authors: Wu Shin-Da , Chuang Wei-Tsung , Ho Jo-Chen , Wu Hsuan-Chen , Hsu Shan-hui
Published: April 2023

Self-healing properties, originating from the natural healing process, are highly desirable for the fitness-enhancing functionality of biomimetic materials. Herein, we fabricated the biomimetic recombinant spider silk by genetic engineering, in which Escherichia coli (E. coli) was employed as a heterologous expression host. The self-assembled recombinant spider silk hydrogel was obtained through the dialysis process (purity > 85%). The recombinant spider silk hydrogel with a storage modulus of ~250 Pa demonstrated autonomous self-healing and high strain-sensitive properties (critical strain ~50%) at 25 °C. The in situ small-angle X-ray scattering (in situ SAXS) analyses revealed that the self-healing mechanism was associated with the stick-slip behavior of the β-sheet nanocrystals (each of ~2–4 nm) based on the slope variation (i.e., ~−0.4 at 100%/200% strains, and ~−0.9 at 1% strain) of SAXS curves in the high q-range. The self-healing phenomenon may occur through the rupture and reformation of the reversible hydrogen bonding within the β-sheet nanocrystals. Furthermore, the recombinant spider silk as a dry coating material demonstrated self-healing under humidity as well as cell affinity. The electrical conductivity of the dry silk coating was ~0.4 mS/m. Neural stem cells (NSCs) proliferated on the coated surface and showed a 2.3-fold number expansion after 3 days of culture. The biomimetic self-healing recombinant spider silk gel and thinly coated surface may have good potential in biomedical applications.
Preparation and Orthogonal Analysis for Dual-Responsive Electrochromic Polymer Dispersed Liquid Crystal Devices
Authors: Xian Haiyu , Li Lin , Ding Yilei , Chu Mingjing , Ye Changqing
Published: April 2023

In this work, we provide a fabrication method for dual-responsive electrochromic (EC) polymer dispersed liquid crystal (PDLC) devices. The EC PDLC device was developed by combing the PDLC technique and a colored complex formed via a redox reaction without a specific EC molecule in a simple preparation method. The mesogen played dual roles in the device for scattering in the form of microdroplets and participating in the redox reactions. Orthogonal experiments were performed with the acrylate monomer concentration, the ionic salt concentration, and the cell thickness as factors to investigate the electro-optical performance for the achievement of optimized fabrication conditions. The optimized device presented four switchable states modulated by external electric fields. The light transmittance of the device was changed by an alternative current (AC) electric field while the color change was realized by a direct current (DC) electric field. Variations of mesogen and ionic salt species can modulate the color and hue of devices, which solves the disadvantage of a single color for traditional EC devices. This work lays the foundation for realizing patterned multi-colored patterned displays and anti-counterfeiting via screen printing and inkjet printing techniques.
Transreligiosity and the Messiness of Religious and Social Worlds: Towards a Deleuzian Methodological Imagination for Religious Studies
Authors: Tremlett Paul-François
Published: April 2023

Research methods and concepts in religious studies are conventionally understood as procedures and rules for representing religious and social worlds. However, religious and social worlds are simultaneously messy, lively and elusive, and arguably transreligious ones are especially so. In this essay I reflect on Panagiotopoulos and Roussou’s concept of “transreligiosity” as a means for re-thinking classical and contemporary methodological debates in religious studies, and for reflecting on methods as social practices.
Flotation Recovery of Sphalerite in Sea Water: A Feasibility Study
Authors: Nikolaev Alexander A.
Published: April 2023

Mining and mineral processing industry adversely affects ecosystems and communities in nearby areas, including high freshwater consumption and scarcity. That is why the emerging global trend is to use sea water in flotation to recover valuable minerals from finely disseminated base metals ores. Recent studies investigate sea water flotation of copper, molybdenum, nickel sulphides and pyrite, while flotation of sphalerite, the main valuable mineral for zinc production, remains uncovered. This paper examines the feasibility of sphalerite flotation by conventional collectors in artificial sea water using a bubble-particles technique and frothless flotation tests. Potassium isopropyl xanthate (PIPX) and sodium isopropyl dithiophosphate (SIDTP) were used as collectors, and copper sulphate was introduced as the activator, while zinc sulphate and sodium sulphide were used as depressants. We examined the most common size fractions of sphalerite: medium (−74 + 44 μm) and fines (−44 μm). The findings showed the feasibility of sphalerite flotation in artificial sea water. We also established correlations between the rate of bubble-particle attachment and the sphalerite flotation recovery resulting in the growth of flotation recovery with the increase of the bubble-particle attachment rate. The results can be used as guidelines in choosing flotation reagents for sphalerite flotation in sea water. Another practical application of the results is the potential for sustainable development of the industrial sector, ecosystems and societies due to the replacement of fresh water by sea water, although further technological and environmental studies are required.
Sea Tide Influence on Ice Flow of David Drygalski’s Ice Tongue Inferred from Geodetic GNSS Observations and SAR Offset Tracking Analysis
Authors: Vittuari Luca , Zanutta Antonio , Lugli Andrea , Martelli Leonardo , Dubbini Marco
Published: April 2023

David Glacier and Drygalski Ice Tongue are massive glaciers in Victoria Land, Antarctica. The ice from the East Antarctic Ice Sheet is drained through the former, and then discharged into the western Ross Sea through the latter. David Drygalski is the largest outlet glacier in Northern Victoria Land, floating kilometers out to sea. The floating and grounded part of the David Glacier are the main focus of this article. During the XXI Italian Antarctic Expedition (2005–2006), within the framework of the National Antarctic Research Programme (PNRA), two GNSS stations were installed at different points: the first close to the grounding line of David Glacier, and the second approximately 40 km downstream of the first one. Simultaneous data logging was performed by both GNSS stations for 24 days. In the latest data processing, the kinematic PPP technique was adopted to evaluate the dominant diurnal components and the very small semi-diurnal variations in ice motion induced by the ocean tide and the mean ice flow rates of both GNSS stations. Comparison of the GNSS time series with predicted ocean tide calculated from harmonic coefficients of the nearest tide gauge stations, installed at Cape Roberts and Mario Zucchelli Station, highlight different local response of the glacier to ocean tide, with a minor amplitude of vertical motion at a point partially anchored at the bedrock close to the grounding line. During low tide, the velocity of the ice flow reaches its daily maximum, in accordance with the direction of seawater outflow from the fjord into the ocean, while the greatest daily tidal excursion generates an increase in the horizontal ice flow velocity. With the aim to extend the analysis in spatial terms, five COSMO-SkyMED Stripmap scenes were processed. The comparison of the co-registered offset tracking rates, obtained from SAR images, with the GNSS estimation shows good agreement.
Automatic Detection of Floating Macroalgae via Adaptive Thresholding Using Sentinel-2 Satellite Data with 10 m Spatial Resolution
Authors: Muzhoffar Dimas Angga Fakhri , Sakuno Yuji , Taniguchi Naokazu , Hamada Kunihiro , Shimabukuro Hiromori , Hori Masakazu
Published: April 2023

Extensive floating macroalgae have drifted from the East China Sea to Japan’s offshore area, and field observation cannot sufficiently grasp their extensive spatial and temporal changes. High-spatial-resolution satellite data, which contain multiple spectral bands, have advanced remote sensing analysis. Several indexes for recognizing vegetation in satellite images, namely, the normalized difference vegetation index (NDVI), normalized difference water index (NDWI), and floating algae index (FAI), are useful for detecting floating macroalgae. Thresholds are defined to separate macroalgae-containing image pixels from other pixels, and adaptive thresholding increases the reliability of image segmentation. This study proposes adaptive thresholding using Sentinel-2 satellite data with a 10 m spatial resolution. We compare the abilities of Otsu’s, exclusion, and standard deviation methods to define the floating macroalgae detection thresholds of NDVI, NDWI, and FAI images. This comparison determines the most advantageous method for the automatic detection of floating macroalgae. Finally, the spatial coverage of floating macroalgae and the reproducible combination needed for the automatic detection of floating macroalgae in Kagoshima, Japan, are examined.
MTCSNet: Mean Teachers Cross-Supervision Network for Semi-Supervised Cloud Detection
Authors: Li Zongrui , Pan Jun , Zhang Zhuoer , Wang Mi , Liu Likun
Published: April 2023

Cloud detection methods based on deep learning depend on large and reliable training datasets to achieve high detection accuracy. There will be a significant impact on their performance, however when the training data are insufficient or when the label quality is low. Thus, to alleviate this problem, a semi-supervised cloud detection method, named the mean teacher cross-supervision cloud detection network (MTCSNet) is proposed. This method enforces both consistency and accuracy on two cloud detection student network branches, which are perturbed with different initializations, for the same input image. For each of the two student branches, the respective teacher branches, used to generate high-quality pseudo labels, are constructed using an exponential moving average method (EMA). A pseudo one-hot label, produced by one teacher network branch, supervises the other student network branch with the standard cross-entropy loss, and vice versa. To incorporate additional prior information into the model, the presented method uses near-infrared bands instead of red bands as model inputs and injects strong data augmentations on unlabeled images fed into the student model. This induces the model to learn richer representations and ensure consistency constraints on the predictions of the same unlabeled image across different batches. To attain a more refined equilibrium between the supervised and semi-supervised loss in the training process, the proposed cloud detection network learns the optimal weights based on homoscedastic uncertainty, thus effectively exploiting the advantages of semi-supervised tasks and elevating the overall performance. Experiments on the SPARCS and GF1-WHU public cloud detection datasets show that the proposed method outperforms several state-of-the-art semi-supervised algorithms when only a limited number of labeled samples are available.
HAFNet: Hierarchical Attentive Fusion Network for Multispectral Pedestrian Detection
Authors: Peng Peiran , Xu Tingfa , Huang Bo , Li Jianan
Published: April 2023

Multispectral pedestrian detection via visible and thermal image pairs has received widespread attention in recent years. It provides a promising multi-modality solution to address the challenges of pedestrian detection in low-light environments and occlusion situations. Most existing methods directly blend the results of the two modalities or combine the visible and thermal features via a linear interpolation. However, such fusion strategies tend to extract coarser features corresponding to the positions of different modalities, which may lead to degraded detection performance. To mitigate this, this paper proposes a novel and adaptive cross-modality fusion framework, named Hierarchical Attentive Fusion Network (HAFNet), which fully exploits the multispectral attention knowledge to inspire pedestrian detection in the decision-making process. Concretely, we introduce a Hierarchical Content-dependent Attentive Fusion (HCAF) module to extract top-level features as a guide to pixel-wise blending features of two modalities to enhance the quality of the feature representation and a plug-in multi-modality feature alignment (MFA) block to fine-tune the feature alignment of two modalities. Experiments on the challenging KAIST and CVC-14 datasets demonstrate the superior performance of our method with satisfactory speed.
Capturing Small-Scale Surface Temperature Variation across Diverse Urban Land Uses with a Small Unmanned Aerial Vehicle
Authors: Ahmad Junaid , Eisma Jessica A.
Published: April 2023

Urbanization increases the urban land surface temperature (LST), challenging society and the environment. This study measured the LST of diverse land uses (LU) in Dallas–Fort Worth (DFW) using a high-resolution (8 cm) thermal infrared sensor onboard a small, unmanned aerial vehicle (UAV). LUs included park (PA), industrial (IA), residential low-cost (RLC), and residential high-cost (RHC) areas. LST was collected by the UAV at different times on eight nonconsecutive days. UAV-collected LST was compared with that from Landsat 8-9 and in situ measurements. RHC reported the highest mean LST, and PA showed the lowest mean LST. Dark-colored asphalt shingle roofs in RHC had the highest mean LST range at 35.67 °C. Lower LST was measured in shaded areas and under thick green cover, whereas areas with thin green cover occasionally reported higher LST than pavements. The micro-urban heat island (MUHI) was calculated between LUs and within land cover types (roof, pavement, green, and water). The MUHI varied from 4.83 °C to 15.85 °C between LUs and 0.2 °C to 23.5 °C within LUs for the less than 1 km2 study area. While the UAV thermal sensor and Landsat demonstrated a similar trend of LST variation, the UAV sensor reported more intense MUHI. An average percent bias (PBIAS) of 5.1% was calculated between the UAV sensor and in situ measurements. This study helps inform the urban design process by demonstrating how land use decisions impact LST locally and provides valuable insight for studies concerned with fine-scale urban LST variability.
An Erosion-Based Approach Using Multi-Source Remote Sensing Imagery for Grassland Restoration Patterns in a Plateau Mountainous Region, SW China
Authors: Chen Guokun , Wang Yiwen , Wen Qingke , Zuo Lijun , Zhao Jingjing
Published: April 2023

Satellite remote sensing of grassland ecosystem restoration requires considering both the above-ground biomass and soil information, and the latter is even more crucial due to the value and restoration difficulty of soil productivity. In this study, we proposed an approach to support the restoration pattern for mountainous grasslands at regional scale. The approach integrates different aspects and key processes, including degradation status, restoration potential and recovery capability, compared to a reference state. Specifically, we illustrated the method with the case of grasslands in southwestern China from a conservation perspective. Soil erosion conditions, net primary productivity and regrowth rate of grasslands were selected as indicators to reveal restoration possibilities. The results showed that the method proposed for remote sensing identification of grassland distribution has an overall accuracy of 88.21% at the regional scale. 59.54% of grasslands in Zhaotong are being eroded with an unsustainable erosion rate greater than the tolerant soil loss, and the average annual soil erosion rate is 952.17 t/(km2·a). Meanwhile, there is obvious spatial heterogeneity in soil erosion factors, vegetation restoration potential and regrowth rate, and the dry–hot valley of Jinsha River in the southwest is much more sensitive to climate change and vulnerable than other regions. The grassland vegetation cover revealed a fluctuating trend and protection of grassland vegetation on soil from erosion has an obvious lag, restoration efforts should be focused on the months before the arrival of the rainy season. In light of various grassland types, the overlay zoning results suggest various restoration patterns of natural repair and manual intervention should be employed for different grasslands. Urgent action is needed to face the challenge and process of grassland degradation and restore its sustainability with shared understanding by taking the stakeholders, collaborations and mutual relationships among different roles into account (e.g., scientist, government and herdsman).
Assessment of Antarctic Sea Ice Cover in CMIP6 Prediction with Comparison to AMSR2 during 2015–2021
Authors: Li Siqi , Zhang Yu , Chen Changsheng , Zhang Yiran , Xu Danya , Hu Song
Published: April 2023

A comprehensive assessment of Antarctic sea ice cover prediction is conducted for twelve CMIP6 models under the scenario of SSP2-4.5, with a comparison to the observed data from the Advanced Microwave Scanning Radiometer 2 (AMSR2) during 2015–2021. In the quantitative evaluation of sea ice extent (SIE) and sea ice area (SIA), most CMIP6 models show reasonable variation and relatively small differences compared to AMSR2. CMCC-CM4-SR5 shows the highest correlation coefficient (0.98 and 0.98) and the lowest RMSD (0.98 × 106 km2 and 1.07 × 106 km2) for SIE and SIA, respectively. In the subregions, the models with the highest correlation coefficient and the lowest RMSD for SIE and SIA are inconsistent. Most models tend to predict smaller SIE and SIA compared to the observational data. GFDL-CM4 and FGOALS-g3 show the smallest mean bias (−4.50 and −1.21 × 105 km2) and the most reasonable interannual agreement of SIE and SIA with AMSR2, respectively. In the assessment of sea ice concentration (SIC), while most models can accurately predict the distribution of large SIC surrounding the Antarctic coastal regions, they tend to underestimate SIC and are unable to replicate the major patterns in the sea ice edge region. GFDL-CM4 and FIO-ESM-2-0 exhibit superior performance, with less bias (less than −5%) and RMSD (less than 23%) for SIC in the Antarctic. GFDL-CM4, FIO-ESM-2-0, and CESM2 exhibit relatively high positive correlation coefficients exceeding 0.60 with the observational data, while few models achieve satisfactory linear trend prediction of SIC. Through the comparison with RMSD, Taylor score (TS) consistently evaluates the Antarctic sea ice cover and proves to be a representative statistical indicator and applicable for its assessment. Based on comprehensive assessments of sea ice cover, CESM2, CMCC-CM4-SR5, FGOALS-g3, FIO-ESM-2-0, and GFDL-CM4 demonstrate more reasonable prediction performance. The assessment findings enhance the understanding of the uncertainties associated with sea ice in the CMIP6 models and highlighting the need for a meticulous selection of the multimodel ensemble.