bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Clinical features and prognosis of two Infants with rhabdomyosarcoma of the tongue
Authors: Yang Peiyi , Xu Na , Su Yan , Duan Chao , Wang Shengcai , Fu Libing , Yu Tong , Guo Ruolan , Ma Xiaoli
Published: January 2023

Background

Rhabdomyosarcoma (RMS) is the most common soft tissue tumor in children, and its most common pathological types include embryonal RMS and alveolar RMS. In contrast, spindle cell RMS (SRMS) is a rare type. Moreover, the tongue is a rare primary site of RMS, and infancy is a rare age at onset.

Case presentation

Two infants were diagnosed with lingual RMS at 3 and 5 months after birth, respectively, and were admitted to Beijing Children’s Hospital. The pathological type in both cases was SRMS. Both were classified as low-risk and were treated with surgery and chemotherapy. Case 1 was in complete remission at the latest follow-up, and Case 2 had a relapse 10 months after stopping chemotherapy, achieving complete remission after the multimodal treatment of chemotherapy, surgery, and radiotherapy. The venous blood gene test of the two infants did not indicate a pathogenic mutation or a possible pathogenic mutation related to RMS. In Case 1, variants of the CDK4 and BRCA1 genes, both with unknown significance and a possible relation to RMS, were detected. In Case 2, three gene variants of unknown significance that were possibly associated with RMS—TRIP13, APC, and RAD54L—were identified.

Conclusion

Lingual RMS in infants is rare. Its clinical manifestations lack specificity, and early recognition is complex. The success and timing of local treatment are important prognostic factors. Genetic testing may be helpful for the early detection of tumor susceptibility and the estimation of prognosis.
Metabolic characteristics of the various incision margins for breast cancer conservation surgery
Authors: Wang Fang , Gu Zongze , Zhao Xunan , Chen Zhuo , Zhang Zhe , Sun Shihao , Han Mingli
Published: January 2023

Background

Breast cancer (BC) has recently become the most prevalent malignancy in women. There are many alternative treatments for BC, and for aesthetic and postoperative quality of life concerns, breast-conserving surgery and corresponding adjuvant therapy have become the predominant treatment for early invasive BC. Currently, the main method used to assess the margins for breast-conserving surgery is intraoperative pathological diagnosis. However, the designation of surgical margins is controversial, and metabolomics may be a novel approach to evaluate surgical margins.

Methods

We collected specimens from 10 breast cancer patients and samples from its surrounding tissues and divided them into cancerous tissue and 1 mm, 2 mm, 3 mm, 5 mm and 10 mm cutting edge tissues, with a total of 60 samples. The samples were analyzed by mass spectrometry on an ultra-performance liquid chromatography-quadrupole/Orbitrap high resolution platform. The data were then statistically analyzed to detect metabolic changes in the different cutting edges and to identify possible surgical cutting edges with statistically significant findings. Abnormal metabolic pathways were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG), which elucidated potential markers.

Results

Statistical analysis indicated that there were substantial differences between the 1 mm margin tissue and the cancer tissue, while there were no statistically significant differences between the 1 mm tissue and tissues from the other margins. The levels of 6 metabolites in the 1 mm tissue were significantly different from those in the cancer tissue and were not significantly different from those in the 2 mm tissue. The six metabolites were pyruvate, N-acetyl-L-aspartate, glutamic acid, γ-aminobutyric acid, fumaric acid, and citric acid. Metabolic pathways such as amino acid metabolism and amino t-RNA synthesis in the margin tissue were significantly distinct from those in cancer tissues based on KEGG analysis.

Conclusion

There was a significant difference between the 1 mm margin tissue and the cancerous tissue. Based on metabolomic analysis, the 1 mm negative margin is sufficient for surgery, and the six metabolites that we identified as abnormal, including pyruvic acid, N-acetyl-L-aspartic acid, glutamic acid, gamma-aminobutyric acid, fumaric acid and citric acid, may serve as biomarkers for a negative margin and help surgeons select an appropriate surgical margin.
Expert guidance on prophylaxis and treatment of dermatologic adverse events with Tumor Treating Fields (TTFields) therapy in the thoracic region
Authors: Anadkat Milan J. , Lacouture Mario , Friedman Adam , Horne Zachary D. , Jung Jae , Kaffenberger Benjamin , Kalmadi Sujith , Ovington Liza , Kotecha Rupesh , Abdullah Huda Ismail , Grosso Federica
Published: January 2023

Tumor Treating Fields (TTFields) are electric fields, delivered via wearable arrays placed on or near the tumor site, that exert physical forces to disrupt cellular processes critical for cancer cell viability and tumor progression. As a first-in-class treatment, TTFields therapy is approved for use in newly diagnosed glioblastoma, recurrent glioblastoma, and pleural mesothelioma. Additionally, TTFields therapy is being investigated in non-small cell lung cancer (NSCLC), brain metastases from NSCLC, pancreatic cancer, ovarian cancer, hepatocellular carcinoma, and gastric adenocarcinoma. Because TTFields therapy is well tolerated and delivery is locoregional, there is low risk of additive systemic adverse events (AEs) when used with other cancer treatment modalities. The most common AE associated with TTFields therapy is mild-to-moderate skin events, which can be treated with topical agents and may be managed without significant treatment interruptions. Currently, there are no guidelines for oncologists regarding the management of TTFields therapy-related skin AEs in the thoracic region, applicable for patients with pleural mesothelioma or NSCLC. This publication aims to provide guidance on preventing, minimizing, and managing dermatologic AEs in the thoracic region to help improve patient quality of life and reduce treatment interruptions that may impact outcomes with TTFields therapy.
Artificial intelligence assists precision medicine in cancer treatment
Authors: Liao Jinzhuang , Li Xiaoying , Gan Yu , Han Shuangze , Rong Pengfei , Wang Wei , Li Wei , Zhou Li
Published: January 2023

Cancer is a major medical problem worldwide. Due to its high heterogeneity, the use of the same drugs or surgical methods in patients with the same tumor may have different curative effects, leading to the need for more accurate treatment methods for tumors and personalized treatments for patients. The precise treatment of tumors is essential, which renders obtaining an in-depth understanding of the changes that tumors undergo urgent, including changes in their genes, proteins and cancer cell phenotypes, in order to develop targeted treatment strategies for patients. Artificial intelligence (AI) based on big data can extract the hidden patterns, important information, and corresponding knowledge behind the enormous amount of data. For example, the ML and deep learning of subsets of AI can be used to mine the deep-level information in genomics, transcriptomics, proteomics, radiomics, digital pathological images, and other data, which can make clinicians synthetically and comprehensively understand tumors. In addition, AI can find new biomarkers from data to assist tumor screening, detection, diagnosis, treatment and prognosis prediction, so as to providing the best treatment for individual patients and improving their clinical outcomes.
Analysis of AVPR1A, thermal and pressure pain thresholds, and stress in sickle cell disease
Authors: Powell-Roach Keesha L. , Yao Yingwei , Cao Xueyuan , Chamala Srikar , Wallace Margaret R. , Cruz-Almeida Yenisel , Molokie Robert E. , Wang Zaijie Jim , Wilkie Diana J.
Published: January 2023

Aim

In patients with sickle cell disease (SCD), negative physical and emotional experiences result from intense chronic and acute pain episodes, but factors underlying these, and their interactions, are not well understood. The arginine vasopressin receptor 1a gene (AVPR1A) single nucleotide polymorphism rs10877969 has been previously associated with aspects of acute pain and stress related pain. In this study, we tested for associations between this SNP, thermal and pressure pain thresholds, clinical pain, and stress in people with SCD.

Methods

150 adults enrolled with SCD completed pain intensity measures (Average Pain Intensity, API) and the Perceived Stress Questionnaire (PSQ). Thermal and pressure pain threshold data were available from quantitative sensory testing (QST), and rs10877969 genotypes were obtained.

Results

In models adjusted for age and gender, between rs10877969 genotypes, we observed no significant differences in thermal (cold, p = 0.66; heat, p = 0.91) and mechanical (pressure, p = 0.33) pain thresholds. The association of rs10877969 with API (p = 0.09) was borderline, but non-significant with PSQ (p = 0.51). The correlation between clinical pain and environmental stress was significant, r = 0.18, p = 0.024, however, the interaction of genotype and PSQ was not significant (p = 0.63).

Conclusion

Clinical and experimental pain were not significantly associated with the rs10877969 genotype. The rs10877969 genotype did not moderate the correlation between environmental stress and clinical pain in this population. However, a trend toward a protective T allele effect on average pain rating in SCD warrants future exploration of this SNP/gene in SCD.
Heart rate variability analysis for the prediction of EEG grade in infants with hypoxic ischaemic encephalopathy within the first 12 h of birth
Authors: Pavel Andreea M , Mathieson Sean R , Livingstone Vicki , O’Toole John M , Pressler Ronit M , de Vries Linda S , Rennie Janet M , Mitra Subhabrata , Dempsey Eugene M , Murray Deirdre M , Marnane William P , Boylan Geraldine B ,   ANSeR Consortium
Published: January 2023

Background and aims

Heart rate variability (HRV) has previously been assessed as a biomarker for brain injury and prognosis in neonates. The aim of this cohort study was to use HRV to predict the electroencephalography (EEG) grade in neonatal hypoxic-ischaemic encephalopathy (HIE) within the first 12 h.

Methods

We included 120 infants with HIE recruited as part of two European multi-centre studies, with electrocardiography (ECG) and EEG monitoring performed before 12 h of age. HRV features and EEG background were assessed using the earliest 1 h epoch of ECG-EEG monitoring. HRV was expressed in time, frequency and complexity features. EEG background was graded from 0-normal, 1-mild, 2-moderate, 3-major abnormalities to 4-inactive. Clinical parameters known within 6 h of birth were collected (intrapartum complications, foetal distress, gestational age, mode of delivery, gender, birth weight, Apgar at 1 and 5, assisted ventilation at 10 min). Using logistic regression analysis, prediction models for EEG severity were developed for HRV features and clinical parameters, separately and combined. Multivariable model analysis included 101 infants without missing data.

Results

Of 120 infants included, 54 (45%) had normal-mild and 66 (55%) had moderate-severe EEG grade. The performance of HRV model was AUROC 0.837 (95% CI: 0.759–0.914) and clinical model was AUROC 0.836 (95% CI: 0.759–0.914). The HRV and clinical model combined had an AUROC of 0.895 (95% CI: 0.832–0.958). Therapeutic hypothermia and anti-seizure medication did not affect the model performance.

Conclusions

Early HRV and clinical information accurately predicted EEG grade in HIE within the first 12 h of birth. This might be beneficial when EEG monitoring is not available in the early postnatal period and for referral centres who may want some objective information on HIE severity.
The safety and effectiveness of Santulli enterostomy in neonatal intestinal conditions
Authors: Yue Ming , Yang Heying , Cui Mingxia , Yuan Yuhang , Zhang Ning , Zhang Xiangyu , Li Yan
Published: January 2023

Background

As an end stoma, Santulli enterostomy provides early restoration of intestinal continuity without formal laparotomy. Short amputation of the common limb enables closure on a side to restore anatomic continuity without sacrificing valuable intestine; additionally, the procedure is simple and safe. Most newborns who require enterostomy might benefit from Santulli enterostomy; however, several pediatric surgeons lack information regarding this procedure. Therefore, we have reviewed our experience about Santulli enterostomy and explore the advantages and indications in neonatal intestinal conditions.

Methods

The clinical data of 76 neonates who underwent enterostomywere obtained. The patients were divided into two groups: the Santulli group with 33 cases who underwent Santulli enterostomy, and the control group with 43 cases who underwent double- or single-lumen ostomy. The general data of the two groups were analyzed, and the perioperative/postoperative complications, clinical data and the long-term outcomes were compared.

Results

There was no difference in the demographic informations, the level of enterostomy, the rate of high-sight stoma, the operative time and bleeding of enterostomy between the two groups. Compared to the control group, the operative time of ostomy closure was less in the Santulli group (53.00 vs. 152.47, P < 0.001). The duration of parenteral nutrition (27.45 vs. 44.56, P = 0.010), the mean interval of initial enterostomy to stomal closure (131.21 vs. 216.42, P < 0.001), and length of stay (46.00 vs. 67.60, P = 0.007) were shorter, while the incidence of postoperative complications and hospitalization costs (11.21 vs. 15.49, P = 0.006) were lower. The Santulli procedure can reduce the morbidity of high output ostomy (2 vs. 10, P = 0.042) and short bowel syndrome (3 vs. 132, P = 0.025), shorten the discrepancy of diameter between the proximal and distal segments, maximize the available intestine, and monitor the movement of the distal bowel. The length of incision was shorter, and the catch-up growth was significantly faster in the Santulli group.

Conclusion

Santulli enterostomy is a superior procedure in the treatment of neonatal intestinal conditions, in terms of fewer complications, faster catch-up growth, shorter hospitalization time and treatment duration. It should be the procedure of choice in several newborns with intestinal conditions that require ostomy.
Delayed vs. early enteral feeding after repair of congenital recto-vestibular fistula: The effect on perineal wound healing
Authors: Kurdi Mazen , Moukhtar Ahmed , Elkholy Mahmoud , Alwassia Heidi , Bamehriz Maha , Khirallah Mohammad Gharieb
Published: January 2023

Introduction

congenital Recto vestibular fistula represents the commonest type of anorectal malformation in females. The treatment of this anomaly is mainly approached either through anterior or posterior sagittal ano-rectoplasty approach. Several perioperative factors may affect the outcome. One of major postoperative complications is the occurrence of wound infection. We aimed to study the effect of delayed vs. early enteral feeding on the occurrence of perineal wound infection (PWI) after repair of congenital recto vestibular fistula.

Patients and methods

Fifty-five infants with recto-vestibular fistula were included. They were managed by single stage anterior sagittal anorectoplasty (ASARP) at an age ≥3 months. Groups A and B included infants who started oral intake on the 6th and 2nd postoperative days respectively. Group A infants were kept on peripheral parenteral nutrition (PPN) during the fasting period.

Results

Superficial wound infection occurred in three cases in group A while it developed in seven cases in group B. Deep perineal infection occurred in two and five cases in group A and group B respectively. The mean hospital stay was 8 days in group A vs. 13 days in group B when PWI developed.

Conclusion

Delayed enteral feeding with PPN keeps the perineal wound less contaminated with stool. This promoted proper and fast healing with lower incidence of PWI. Also, PPN compensates the catabolic effects of both surgical trauma and fasting during the postoperative period and ensures maintenance of normal levels of essential nutrients that allow for proper healing.
The involvement of the noradrenergic system in the antinociceptive effect of cucurbitacin D on mice with paclitaxel-induced neuropathic pain
Authors: Park Keun-Tae , Kim Suyong , Choi Ilseob , Han Ik-Hwan , Bae Hyunsu , Kim Woojin
Published: January 2023

Paclitaxel (sold under the brand name Taxol) is a chemotherapeutic drug that is widely used to treat cancer. However, it can also induce peripheral neuropathy, which limits its use. Although several drugs are used to attenuate neuropathy, no optimal treatment is available to date. In this study, the effect of cucurbitacins B and D on paclitaxel-induced neuropathic pain was assessed. Multiple paclitaxel injections (a cumulative dose of 8 mg/kg, i. p.) induced cold and mechanical allodynia from days 10 to 21 in mice, and the i. p. administration of 0.025 mg/kg of cucurbitacins B and D attenuated both allodynia types. However, as cucurbitacin B showed a more toxic effect on non-cancerous (RAW 264.7) cells, further experiments were conducted with cucurbitacin D. The cucurbitacin D dose-dependently (0.025, 0.1, and 0.5 mg/kg) attenuated both allodynia types. In the spinal cord, paclitaxel injection increased the gene expression of noradrenergic (α1-and α2-adrenergic) receptors but not serotonergic (5-HT1A and 3) receptors. Cucurbitacin D treatment significantly decreased the spinal α1- but not α2-adrenergic receptors, and the amount of spinal noradrenaline was also downregulated. However, the tyrosine hydroxylase expression measured via liquid chromatography in the locus coeruleus did not decrease significantly. Finally, cucurbitacin D treatment did not lower the anticancer effect of chemotherapeutic drugs when co-administered with paclitaxel in CT-26 cell-implanted mice. Altogether, these results suggest that cucurbitacin D could be considered a treatment option against paclitaxel-induced neuropathic pain.
Psilocybin sex-dependently reduces alcohol consumption in C57BL/6J mice
Authors: Alper Kenneth , Cange Janelle , Sah Ria , Schreiber-Gregory Deanna , Sershen Henry , Vinod K. Yaragudri
Published: January 2023

The classical psychedelic psilocybin is of interest as a treatment for alcohol use disorder (AUD). This study investigated the effects of psilocybin on voluntary ethanol consumption in adult male and female C57BL/6J mice administered saline or psilocybin intraperitoneally as a single dose of 0.1, 0.5, 1.0 or 2.0 mg/kg and provided 20% ethanol utilizing a two-bottle choice alcohol drinking paradigm. Ethanol was provided continuously for 3 days immediately following the administration of psilocybin, then withheld for 2 days, and then provided continuously for two subsequent additional days. A multilevel model (MLM) for repeated measures was used to compare ethanol consumption and preference in psilocybin-treated groups versus controls. Ethanol consumption and preference were reduced in male mice during the 3-day interval that immediately followed psilocybin administration. The effect of psilocybin on ethanol consumption was dose-related and was consistent across the 3-day interval at dosages of 0.5 mg/kg or greater. Psilocybin had no effect on consumption or preference when ethanol was subsequently reintroduced after 2 days of withdrawal. In contrast to males, psilocybin had no significant effect on ethanol consumption or preference in female mice at any dosage or time point. The lack of an effect of psilocybin on quinine preference, and its limited interaction with locomotor activity indicated that the observed reduction in voluntary ethanol consumption was not attributable to altered taste perception or motor effects. Total fluid consumption was increased in males at some time points and psilocybin dosages and unchanged in females, and the absence of any decrease in either group at any time point indicated that the observed reduction in ethanol consumption was not mediated by nonspecific effects on consummatory behavior. The finding of a sex-dependent effect of psilocybin on ethanol consumption suggests that the C57BL/6J mouse may provide a useful experimental approach to modeling sex differences in vulnerability to AUD in addition to investigation of the neurobiological basis of the effect of classical psychedelics on alcohol drinking behavior.
Comparative efficacy of Chinese patent medicines for non-alcoholic fatty liver disease: A network meta-analysis
Authors: Zhang Lihui , Liu Sutong , Gu Yajiao , Li Shanzheng , Liu Minghao , Zhao Wenxia
Published: January 2023

Background: The incidence of Non-alcoholic fatty liver disease (NAFLD) is increasing year by year. Researches showed that Chinese patent medicines (CPMs) had achieved good efficacy in the treatment of Non-alcoholic fatty liver disease. However, the debate on optimum Chinese patent medicine (CPM) persists. Therefore, we conducted a network meta-analysis to objectively compare the efficacy of different Chinese patent medicines in the treatment of Non-alcoholic fatty liver disease.

Methods: PubMed, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure, Wanfang Database, China Science and Technology Journal Database, and Chinese Biomedical Literature Database were used as databases for RCT researches retrieval. The retrieval time was from establishment of the database to July 2022. After effective data was extracted, Review Manager 5.4 and Cochrane Collaboration System Evaluator’s Manual were used to assess bias risk. STATA 16.0 based on frequency theory was used for the network meta-analysis.

Results: Totally 39 studies were included, involving 13 Chinese patent medicines, including 4049 patients, of which 42 patients were lost. In terms of improving clinical efficiency rate, Zhibitai capsule was most likely the best choice of Chinese patent medicine for Non-alcoholic fatty liver disease. Liuwei Wuling tablet had the best effect in reducing serum ALT and AST; Gandan Shukang capsule had the best effect in reducing serum GGT; Qianggan capsule had the best effect in reducing serum TG; Dangfei Liganning capsule had the best effect in reducing serum TC. None of the included studies had serious adverse reactions.

Conclusion: For patients with Non-alcoholic fatty liver disease in this NMA, Zhibitai capsule, Liuwei Wuling tablet, Gandan Shukang capsule, Qianggan capsule, Dangfei Liganning capsule might be noteworthy. Due to the uclear risk bias, better designed double-blind, multi center and large sample RCTs are needed which resolve the problems of blinding, selective reporting and allocation concealment.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42022341240.
Integrated bioinformatical and in vitro study on drug targets for liver cirrhosis based on unsupervised consensus clustering and immune cell infiltration
Authors: Chi Qingjia , Wang Di , Sun Ting , Liang Hua-Ping
Published: January 2023

Liver cirrhosis is one of the most common cause of death in the world. The progress of liver cirrhosis involves health, liver cirrhosis and liver cancer, leading to great challenges in the diagnosis of the disease. Drug targets, which could be obtained conveniently, can help clinicians improve prognosis and treatment. Liver cirrhosis is associated with serum calcium levels. And studies reported Tanshinone IIA plays a therapeutic role in liver injury through activating calcium-dependent apoptosis. In this study, we explored the diagnostic key targets of Tanshinone IIA in liver cirrhosis through exploration of comprehensive dataset including health, liver cirrhosis and liver cancer patients. The unsupervised consensus clustering algorithm identified 3 novel subtypes in which differentially expressed genes (DEGs) between both subtypes were found by pairwise comparison. Then, 4 key drug targets of Tanshinone IIA were determined through the intersection of these DEGs. The diagnostic performance of target genes was assessed and further verified in the external dataset. We found that the 4 key drug targets could be used as effective diagnostic biomarkers. Then the immune scores in the high and low expression groups of target genes were estimated to identify significantly expressed immune cells. In addition, the immune infiltration of high and low target gene expression groups in several immune cells were significantly different. The findings suggest that 4 key drug targets may be a simple and useful diagnostic tool for predicting patients with cirrhosis. We further studied the carcinogenesis role of AKR1C3 and TPX2 in vitro. Both mRNA and protein expression in hepatoma carcinoma cells was detected using qRT-PCR and Western blot. And the knockdown of AKR1C3 and TPX2 significantly suppressed cell proliferation, migration and invasion.
A representation and classification method for collective investor attention in the financial market
Authors: Yang Zhen-Hua , Su Bo , Wang Zi-Yi , Zhu Xi-Hua , Liu Jian-Guo
Published: January 2023

Introduction: It is increasingly becoming integral to analyze the collected information effectively.

Methods: We propose a representation and classification method for collective investor attention in the financial market, taking the Chinese stock market as an example. The method includes three key steps: 1) converting the hourly search volume of each stock per week to an image representation for describing the changes of collective investor attention; 2) extracting features of each image by utilizing a self-encoding algorithm in deep learning; and 3) clustering generated images by K-means to arrange stocks into different groups.

Results: The empirical results show that the portfolio considering the clustering information outperforms the HS300 index.

Discussion: The method may not only use deep learning features for stock similarity measurement, but also shed some light on profoundly understanding the mechanisms of the collective investor attention for the financial market.
Design of a transportable miniaturized optical reference cavity with flexibly tunable thermal expansion properties
Authors: Zhao Weinan , Wu Hanxu , Fu Yang , Ge Jun , Yang Honglei , Zhang Shengkang
Published: January 2023

A 3-cm-long optical reference cavity for transportable miniaturized ultra-stable laser is designed and analyzed using finite element analysis (FEA). Although the tiny cavity is formed in a conventional way, in which a cylinder spacer made of ultra-low expansion (ULE) glass is optically contacted with fused-silica mirror substrates and compensation rings, the compensation rings are specially designed in order to broaden the zero-thermal-expansion temperature tuning range. In addition, the cavity is capable of being rigidly fixed by clamping both end sections of the cylinder spacer along the axis. The thermodynamic analysis shows that a larger tuning span of the zero-thermal-expansion temperature varying from −10 K to + 23 K compared to all-ULE cavity is benefited, resulting in the whole optical reference cavity could work around room temperature. Meanwhile, the statics analysis indicates the design is insensitive to extrusion force and vibration so that it owns a potential of solid performance after transportation.
Rich dynamics of a vegetation–water system with the hydrotropism effect
Authors: Li Li , Wang Fang-Guang , Hou Li-Feng
Published: January 2023

In recent years, with the abnormal global climate change, the problem of desertification has become more and more serious. The vegetation pattern is accompanied by desertification, and thus, the study of the vegetation pattern is helpful to better understand the causes of desertification. In this work, we reveal the influences of hydrotropism on the vegetation pattern based on a vegetation–water system in the form of reaction–diffusion equations. Parameter ranges for the steady-state mode obtained by analyzing the system show the dynamic behavior near the bifurcation point. Furthermore, we found that vegetation hydrotropism not only induces spatial pattern generation but also promotes the growth of vegetation itself in this area. Therefore, through the study of vegetation patterns, we can take corresponding preventive measures to effectively prevent land desertification and improve the stability of the ecosystem in the region.
Calibration of a collinear backscattering Mueller matrix imaging system
Authors: Zhou Xinrong , Fu Yao , Liao Helin , Chen Zhenhua , Ji Yanhong , Tang Zhilie
Published: January 2023

A collinear backscattering Mueller matrix (CBMM) imaging system has clear advantages in the detection of bulk biological tissues, which are highly scattering and depolarizing. Due to the double-pass configuration and noise in the system, the calibration of a collinear backscattering Mueller matrix imaging system is usually complex and of poor accuracy. In this work, we propose an alternative modified eigenvalue calibration method (ECM) based on the equivalent standard sample. For better noise suppression and higher calibration accuracy, we design the distribution of polarization states over the Poincaré sphere and solve for the parameters of equivalent standard samples by means of an optimization. Compared to other variants of the eigenvalue calibration method used in the double-pass system, the accuracy of the proposed method is improved by more than 40 times. The comparison results with the error model-based calibration methods indicate that the modified eigenvalue calibration method generally gives the best accuracy and precision, as well as the best reliability.
Resting-state heart rate variability after stressful events as a measure of stress tolerance among elite performers
Authors: Miyatsu Toshiya , Smith Briana M. , Koutnik Andrew P. , Pirolli Peter , Broderick Timothy J.
Published: January 2023

Introduction: A common trait of elite performers is their ability to perform well when stressed by strong emotions such as fear. Developing objective measures of stress response that reliably predict performance under stress could have far-reaching implications in selection and training of elite individuals and teams. Prior data suggests that (i) Heart rate and heart rate variability (HR/HRV) are associated with stress reaction, (ii) Higher basal sympathetic tone prior to stressful events is associated with higher performance, and (iii) Elite performers tend to exhibit greater increase in parasympathetic tone after a stressful event.

Methods: The current study assesses the predictive utility of post-stressful event HR/HRV measures, an under-studied time point in HR/HRV research, in the context of military personnel selection. Specifically, we examined the relationship between a comprehensive set of HR/HRV measures and established questionnaires related to stress tolerance, experimental evaluation of executive function during stress induction, and ecologically valid selection assessment data from a week-long Special Operations Forces selection course (N = 30).

Results: We found that post-stressful event HR/HRV measures generally had strong correlations with the neuroticism facet of the NEO personality inventory as well as the general and distress facets of the defensive reactivity questionnaire. HR/HRV measures correlated reliably with a change in executive function measured as a decrease in verbal fluency with exposure to a well-validated stressor. Finally, we observed a divergent pattern of correlation among elite and non-elite SOF candidates. Specifically, among elite candidates, parasympathetic nervous system (PNS) measures correlated positively and sympathetic nervous system (SNS) measures correlated negatively with evaluation of stress tolerance by experts and peers. This pattern was not present in non-elite candidates.

Discussion: Our findings demonstrate that post-stressful event HR/HRV data provide an objective non-invasive method to measure the recovery and arousal state in direct reaction to the stressful event and can be used as metrics of stress tolerance that could enhance selection of elite individuals and teams.
NCC regulation by WNK signal cascade
Authors: Uchida Shinichi , Mori Takayasu , Susa Koichiro , Sohara Eisei
Published: January 2023

With-no-lysine (K) (WNK) kinases have been identified as the causal genes for pseudohypoaldosteronism type II (PHAII), a rare hereditary hypertension condition characterized by hyperkalemia, hyperchloremic metabolic acidosis, and thiazide-hypersensitivity. We thought that clarifying the link between WNK and NaCl cotransporter (NCC) would bring us new mechanism(s) of NCC regulation. For the first time, we were able to produce a knock-in mouse model of PHAII and anti-phosphorylated NCC antibodies against the putative NCC phosphorylation sites and discover that constitutive activation of NCC and increased phosphorylation of NCC are the primary pathogenesis of the disease in vivo. We have since demonstrated that this regulatory mechanism is mediated by the kinases oxidative stress-response protein 1 (OSR1) and STE20/SPS1-related proline/alanine-rich kinase (SPAK) (WNK–OSR1/SPAK-NCC signaling cascade) and that the signaling is not only important in the pathological condition of PHAII but also plays a crucial physiological role in the regulation of NCC.
Effects of physical activity on clinical and inflammatory markers in diagnosing multiple myeloma patients
Authors: Wang Jiaping , Sheng Lixia , Lai Yanli , Ouyang Guifang , Xu Zhijuan
Published: January 2023

Multiple myeloma (MM) is the second most common hematological disorder. Although several drugs have been developed to treat MM, their efficacy is uncertain. In addition, how normal physical activities can decrease inflammatory responses and clinical biomarkers in MM patients needs to be better defined. Therefore, this study evaluated possible clinical and inflammatory markers to determine the early diagnosis of MM during physical activity. This study selected 30 MM patients with normal or no physical activity with ages of >50 years. This study did not require any specific exercise protocols other than noting patients’ daily life activities and considering them as physical activity for 17 days. Then, blood samples were collected to assess clinical and inflammatory markers. Regarding clinical markers, daily life activities in MM patients decreased their LDH, calcium, and β2-microglobulin levels significantly compared to other clinical biomarkers such as creatine and total protein. Further, this study observed no significant differences between daily life activities of MM patients and normal MM patients regarding levels of immunoglobulins except IgM. Furthermore, IL-6 level was significantly increased with the daily life activities of MM patients, suggesting the role of physical activities in increasing anti-inflammatory response along with altering the biochemical profiles including LDH, calcium and β2-microglobulin in MM patients.
Genetic variation of Amaranthus retroflexus L. and Chenopodium album L. (Amaranthaceae) suggests multiple independent introductions into Iran
Authors: Hamidzadeh Moghadam Shiva , Alebrahim Mohammad Taghi , Mohebodini Mehdi , MacGregor Dana R.
Published: January 2023

Amaranthus retroflexus L. and Chenopodium album L. (Amaranthaceae) are weedy plants that cause severe ecological and economic damage. In this study, we collected DNA from three different countries and assessed genetic diversity using inter-simple sequence repeat (ISSR) markers. Our analysis shows both weed species have low genetic diversity within a population and high genetic diversity among populations, as well as a low value of gene flow among the populations. UPGMA clustering and principal coordinate analysis indicate four distinct groups for A. retroflexus L. and C. album L. exist. We detected significant isolation-by-distance for A. retroflexus L. and no significant correlation for C.album L. These conclusions are based data from 13 ISSR primers where the average percentage of polymorphism produced was 98.46% for A. retroflexus L. and 74.81% for C. album L.These data suggest that each population was independently introduced to the location from which it was sampled and these noxious weeds come armed with considerable genetic variability giving them the opportunity to manifest myriad traits that could be used to avoid management practices. Our results, albeit not definitive about this issue, do not support the native status of C. album L. in Iran.
Systematic analysis of photo/sko-regulated germination and post-germination development of shallow photodormant seeds in Nicotiana tabacum L.
Authors: Liu Qiyuan , Li Zhenhua , Zhang Min , Dong Shuai , Yang Pingping , Zhang Jie , Loades Eddison
Published: January 2023

Introduction

Light is a major environmental factor in regulating germination and post-germination development of shallow photo-dormant seeds in Nicotiana tabacum L. (tobacco). However, its molecular mechanism remains largely unclear.

Methods and results

In this study, we compared the phenotypes of the seeds germinated under light and dark, and systematically investigated their regulatory networks by integrating transcriptomic and proteomic data. Under light, the germination increased ~25%, the length of the hypocotyl shortened ~3 cm, and the apical hook disappeared. 9, 161, 342 differentially expressed genes (DEGs) and 128, 185, 81 differentially expressed proteins (DEPs) were regulated by light in the development stage of seed imbibition, radicle protrusion and cotyledon expansion respectively. 0, 19 and 1 co-up-regulated and 1, 30 and 64 co-down-regulated DEGs (DEP) were observed in the three stages, respectively. Of them, 2S albumin large chain, was down-regulated by light in imbibed seed. Oleosin 18.5 kDa (OLEO1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPA1), Oxygen-evolving enhancer protein 1-1 and anchloroplastic (PSBO1), hub genes (proteins) in protein-protein interaction network (PPI), were downregulated and up-regulated in germinated seeds by light, respectively. OLEO1, a hub gene (proteins), was down-regulated by light in post-germination seedling.

Conclusion

These results systematically revealed the molecular networks regulated by light during germination and post-germination development of shallow photo-dormant tobacco seeds.
Effect of planting and mowing cover crops as livestock feed on soil quality and pear production
Authors: Fu Haoran , Chen Hong , Ma Qingxu , Han Kefeng , Wu Shaofu , Wu Lianghuan
Published: January 2023

Introduction

The increasing demand for animal-products has led to an increasing demand for livestock feed. Using cover crop as green manure in orchards is an effective measure to improve fruit yield and quality. However, the effect of mowing cover forage crops as livestock feed on soil quality and crop production is unclear.

Method

Therefore, a 4-year field experiment, which included two treatments, was conducted in pear orchards in Luniao County, China: natural grass (NG) and planting and mowing forage crop ryegrass as livestock feed (MF).

Results

Under MF treatment, most soil nutrient content, especially alkalihydrolysable N (AN), total phosphate (TP), available phosphate (AP), and microbial biomass phosphate (MBP), had decreased significantly (P<0.05), while β-D-glucosidase (BG, C-cycle enzyme) and soil C limitation at 10–20 cm depth and P limitation at subsoil (20–40 cm) was increased. In addition, the soil bacterial community component in topsoil (0–10 cm and 10–20 cm) and fungal community component in topsoil and subsoil were changed in the MF treatment. Network analysis showed that MF treatment had a lower edge number in topsoil but the community edge numbers increased from 12794 in NG to 13676 in MF in subsoil. The average weight degree of the three soil layers in MF treatment were reduced, but the modularity had increased than that in NG. For crop production, MF treatment was 1.39 times higher in pear yield and titratable acids (AC) reduced from 0.19% to 0.13% compared with NG. These changes were more associated with the indicators at the subsoil, especially for TP, AN, pH, and F-NMDS1 (non-metric multidimensional scaling (NMDS) axis 1 of fungi).

Discussion

These results provide data support for the feasibility of planting and mowing forage crops as livestock feed on orchards as well as a new idea for the integration of crop and livestock.
The functional analysis of sugar transporter proteins in sugar accumulation and pollen tube growth in pummelo (Citrus grandis)
Authors: Xu Weiwei , Liu Ziyan , Zhao Zeqi , Zhang Shuhang , Li Mengdi , Guo Dayong , Liu Ji-Hong , Li Chunlong
Published: January 2023

Sugar transporter proteins (STPs) play vital roles in sugar transport and allocation of carbon sources in plants. However, the evolutionary dynamics of this important gene family and their functions are still largely unknown in citrus, which is the largest fruit crop in the world. In this study, fourteen non−redundant CgSTP family members were identified in pummelo (Citrus grandis). A comprehensive analysis based on the biochemical characteristics, the chromosomal location, the exon–intron structures and the evolutionary relationships demonstrated the conservation and the divergence of CgSTPs. Moreover, CgSTP4, 11, 13, 14 were proofed to be localized in plasma membrane and have glucose transport activity in yeast. The hexose content were significantly increased with the transient overexpression of CgSTP11 and CgSTP14. In addition, antisense repression of CgSTP4 induced the shorter pollen tube length in vitro, implying the potential role of CgSTP4 in pummelo pollen tube growth. Taken together, this work explored a framework for understanding the physiological role of CgSTPs and laid a foundation for future functional studies of these members in citrus species.
Association between strategic differentiation and firm leverage manipulation: Empirical evidence from China
Authors: Niu Feng , Wang Jiayi , Su Wunhong
Published: January 2023

Introduction

Based on the context of the implementation of China’s comprehensive deleveraging policy, this study explores the impact of firm strategic differentiation on the emergence of leverage manipulation behavior.

Methodology

The A-share listed firms in Shanghai and Shenzhen from 2007 to 2020 were used as the research objects, and the data were processed using Stata software.

Results

The greater the degree of strategic differentiation, the higher the likelihood of firm leverage manipulation. The effect of strategic differentiation on leverage manipulation is more significant when firms are under short-term debt service pressure. Auditor industry expertise can weaken the positive relationship between strategic differentiation and firm leverage manipulation. Further results show that the degree of strategic differentiation increases the likelihood of firm leverage manipulation by increasing the degree of financing constraints of the firm.

Conclusion

This study enriches the literature on leverage manipulation, provides empirical evidence on the economic consequences of strategic differentiation, and has important implications for the precise implementation of deleveraging policies by relevant government departments, as well as a reference for external investors’ decisions.
Visual analysis of environmental research progress in Germany linking Development Anthropology: A sustainable approach based on the Web of Science
Authors: Zong Wei , Wang Fei , Tu Bo , Tu Huazhong , Zhang Hao , Wu Chuke , Toseef Muhammad
Published: January 2023

Environmental devaluation is a major concern for European countries as they seek to scrutinize strategies for development anthropology. Germany holds diversified ties with the socioeconomic and environmental development of the region. In accordance with global obligations, Germany's research on environmental issues, protection laws and actions, and universities and scientific research institutions in the field of environmental protection are progressing toward the development of a sustainable future securing the development anthropology. However, Germany's research on environmental issues is unclear to the rest of the world. Chinese scholars also lack effective countermeasures and suggestions for implementing environmental protection cooperation between China and Germany under the Belt and Road Initiative to draw a sustainable global drain. Understanding the current situation and frontier trend of environmental research in German academic circles is essential and irreplaceable for relying on research results data and quantitative analysis theory to carry out the research process. The methodology of this paper established a quantitative analysis based on “institutions,” “scholars,” “research objects,” and “frequency of keywords” among the research results on environmental issues in Germany. It constructs a digital portrait of the field of environmental research in Germany. Knowledge mapping is extensively used in this study as the primary research tool to show the development of environmental research in Germany. The standard deviation of social science research has roughly doubled in that time. CiteSpace, a visual tool for document statistical analysis, is used to analyze the research results on environmental protection published by German scholars from 2008 to 2018. The study results include Web of Science Network, and finally, a visual map is drawn. This study analyzes the status quo, research institutions, keywords, research hotspots, and research trends of international cooperation in environmental research in Germany. The findings are in supportive position of environment study that is the key to human existence and societal development. Leading to this Germans are in concern of human anthropology being reflected in scholarly published work. In response to practical challenges, “global warming” and “sustainable development” became the most frequently used keywords. It provides sustainable thoughts and countermeasures to strengthen Sino-German environmental protection exchange and cooperation further.
Documenting the mental health climate in correctional work and the realities of suicide
Authors: Johnston Matthew S. , Ricciardelli Rosemary
Published: January 2023

Public safety personnel are at an elevated risk for suicidal thoughts and behaviors relative to the general public. Correctional workers in particular report some of the highest prevalence of suicidal thoughts and behaviors. To better understand this phenomenon, the current study draws on qualitative, open-ended survey response data (n = 94) that explores three distinct themes (occupational environment, lack of support, social silence) and how entrenched notions of mental health stigma and occupational culture inform how Canadian correctional workers understand their experiences with suicidal thoughts and behaviors. We conclude with a brief discussion of the research and policy implications, with an emphasis on mobilizing efforts to normalize mental health discussion in correctional workplaces, bolstering peer support resources, and collaboration, and assessing the limited organizational supports available to struggling staff.