bluuebunny/crossref_metadata_2025 huggingface dataset:
Social Referencing in the Domestic Horse
Authors: Schrimpf Anne , Single Marie-Sophie , Nawroth Christian
Published: January 2020

Dogs and cats use human emotional information directed to an unfamiliar situation to guide their behavior, known as social referencing. It is not clear whether other domestic species show similar socio-cognitive abilities in interacting with humans. We investigated whether horses (n = 46) use human emotional information to adjust their behavior to a novel object and whether the behavior of horses differed depending on breed type. Horses were randomly assigned to one of two groups: an experimenter positioned in the middle of a test arena directed gaze and voice towards the novel object with either (a) a positive or (b) a negative emotional expression. The duration of subjects’ position to the experimenter and the object in the arena, frequency of gazing behavior, and physical interactions (with either object or experimenter) were analyzed. Horses in the positive condition spent more time between the experimenter and object compared to horses in the negative condition, indicating less avoidance behavior towards the object. Horses in the negative condition gazed more often towards the object than horses in the positive condition, indicating increased vigilance behavior. Breed types differed in their behavior: thoroughbreds showed less human-directed behavior than warmbloods and ponies. Our results provide evidence that horses use emotional cues from humans to guide their behavior towards novel objects.
Measurement of Fecal Testosterone Metabolites in Mice: Replacement of Invasive Techniques
Authors: Auer Kerstin E. , Kußmaul Marius , Möstl Erich , Hohlbaum Katharina , Rülicke Thomas , Palme Rupert
Published: January 2020

Testosterone is the main reproductive hormone in male vertebrates and conventional methods to measure testosterone rely on invasive blood sampling procedures. Here, we aimed to establish a non-invasive alternative by assessing testosterone metabolites (TMs) in fecal and urinary samples in mice. We performed a radiometabolism study to determine the effects of daytime and sex on the metabolism and excretion pattern of radiolabeled TMs. We performed physiological and biological validations of the applied EIA to measure TMs and assessed diurnal fluctuations in TM excretions in male and female mice and across strains. We found that males excreted significantly more radiolabeled TMs via the feces (59%) compared to females (49.5%). TM excretion patterns differed significantly between urinary and fecal samples and were affected by the daytime of ³H-testosterone injection. Overall, TM excretion occurred faster in urinary than fecal samples. Peak excretion of fecal TMs occurred after 8 h when animals received the 3H-testosterone in the morning, or after 4 h when they received the 3H-testosterone injection in the evening. Daytime had no effect on the formed TMs; however, males and females formed different types of TMs. As expected, males showed higher fecal TM levels than females. Males also showed diurnal fluctuations in their TM levels but we found no differences in the TM levels of C57BL/6J and B6D2F1 hybrid males. Finally, we successfully validated our applied EIA (measuring 17β-hydroxyandrostane) by showing that hCG (human chorionic gonadotropin) administration increased TM levels, whereas castration reduced them. In conclusion, our EIA proved suitable for measuring fecal TMs in mice. Our non-invasive method to assess fecal TMs can be widely used in various research disciplines like animal behavior, reproduction, animal welfare, ecology, conservation, and biomedicine.
Myostatin (MSTN) Gene Indel Variation and Its Associations with Body Traits in Shaanbei White Cashmere Goat
Authors: Bi Yi , Feng Bo , Wang Zhen , Zhu Haijing , Qu Lei , Lan Xianyong , Pan Chuanying , Song Xiaoyue
Published: January 2020

Myostatin (MSTN) gene, also known as growth differentiation factor 8 (GDF8), is a member of the transforming growth factor-beta super-family and plays a negative role in muscle development. It acts as key points during pre- and post-natal life of amniotes that ultimately determine the overall muscle mass of animals. There are several studies that concentrate on the effect of a 5 bp insertion/deletion (indel) within the 5’ untranslated region (5’ UTR) of goat MSTN gene in goats. However, almost all sample sizes were below 150 individuals. Only in Boer goats, the sample sizes reached 482. Hence, whether the 5 bp indel was still associated with the growth traits of goats in large sample sizes which were more reliable is not clear. To find an effective and dependable DNA marker for goat rearing, we first enlarged the sample sizes (n = 1074, Shaanbei White Cashmere goat) which would enhance the robustness of the analysis and did the association analyses between the 5 bp indel and growth traits. Results uncovered that the 5 bp indel was significantly related to body height, height at hip cross, and chest width index (p < 0.05). In addition, individuals with DD genotype had a superior growing performance than those with the ID genotype. These findings suggested that the 5 bp indel in MSTN gene are significantly associated with growth traits and the specific genotype might be promising for maker-assisted selection (MAS) of goats.
Rumen Fermentation and Fatty Acid Composition of Milk of Mid Lactating Dairy Cows Grazing Chicory and Ryegrass
Authors: Mangwe Mancoba , Bryant Racheal , Gregorini Pablo
Published: January 2020

The goals of the current study were to investigate the effects of including chicory (Cichorium intybus L.) into the traditional feeding regime of ryegrass/white clover (Lolium perenne L./Trifolium repens L.), and time of its allocation on milk production, rumen fermentation, and FA composition of milk and rumen digesta of dairy cows. Nine groups of four cows were allocated one of three replicated feeding regimes: (1) ryegrass/white clover only (RGWC), (2) ryegrass/white clover + morning allocation of chicory (CHAM), and (3) ryegrass/white clover + afternoon allocation of chicory (CHPM). One cow per group had a rumen cannulae fitted. Treatment did not affect total grazing time or estimated dry matter intake, but cows ruminated more when fed RGWC than chicory. Allocating chicory in the afternoon elevated milk production compared with RGWC and CHAM. Milk from cows grazing chicory contained greater concentrations of polyunsaturated FA (PUFA) such as C18:3 c9, 12, 15 and C18:2 c9, 12 than those on RGWC. As with milk, rumen digesta concentration of PUFA increased when cows grazed on chicory rather than RGWC, which corresponded with lower concentrations of intermediate vaccenic and biohydrogenation end-product stearic acid for cows grazing on chicory. Mean ruminal pH was lower for cows offered chicory than those on RGWC, reflecting greater rumen concentrations of volatile fatty acids (VFA) for cows fed chicory. Allocating chicory during the afternoon is a useful strategy that can translate to improved milk production. The lower rumen pH, lower concentration of vaccenic and stearic acids, and elevated concentration of PUFA in the rumen of cows fed chicory suggest reduced biohydrogenation and may explain the elevated concentration of PUFA in the milk of cows fed chicory compared with those fed RGWC.
Assessment of Performance of Two Rapid Methods for On-Site Control of Microbial and Biofilm Contamination
Authors: Lorenzo Fernando , Sanz-Puig Maria , Bertó Ramón , Orihuel Enrique
Published: January 2020

(1) Background: The validation of hygiene procedures in food industries is paramount to ensure that food contact surfaces are properly decontaminated before production. Rapid, sensitive and reliable tools are needed for routine hygiene validation in order to increase food safety levels. Two novel tools for biofilm detection (TBF 300) and detection of low levels of microbial contamination (FreshCheck) have been assessed. (2) Methods: Biofilms of relevant food pathogens: Listeria monocytogenes and Salmonella spp. were grown for 3 and 10 days to assess the performance of the biofilm detection product. Surfaces were inoculated with different levels of L. monocytogenes to determine the limit of detection of FreshCheck. (3) Results: TBF 300 visibly stained 3 days-old biofilms of both pathogens, containing 5.0–5.4 log CFU/cm2. FreshCheck showed a positive reaction with contamination levels as low as 10 CFU/cm2 for L. monocytogenes. (4) Conclusions: Assessment of the hygienic status of food contact surfaces before production can be greatly improved with the use of the two novel tools evaluated in this study. The detection of microorganisms’ presence at very low levels of contamination as well as identification of biofilm growth spots is available in a rapid and easy way, with a big potential contribution to food safety.
Evaluating Centralized and Heterarchical Control of Smart Manufacturing Systems in the Era of Industry 4.0
Authors: Boccella Anna Rosaria , Centobelli Piera , Cerchione Roberto , Murino Teresa , Riedel Ralph
Published: January 2020

In light of the Fourth Industrial Revolution, the concepts of flexibility and re-configurability of manufacturing systems and the evolution of their control architectures are becoming increasingly important. The development of Cyber Physical Systems (CPS) and their flexibility and integrated capabilities have paved the way to the transition from centralized control to heterarchical (decentralized) control architectures. In this paper, a comparison between centralized and heterarchical control architectures in a virtual learning environment is presented. The control architectures of the assembly station and the materials handling system of modern manufacturing systems have been conceptualized and tested under different working conditions. The results show that centralized control is the best solution only for deterministic and predictable scenarios, which are very far from reality, whereas, in case of failures, a more flexible control is preferable.
Novel Approaches to Air Pollution Exposure and Clinical Outcomes Assessment in Environmental Health Studies
Authors: Yarza Shaked , Hassan Lior , Shtein Alexandra , Lesser Dan , Novack Lena , Katra Itzhak , Kloog Itai , Novack Victor
Published: January 2020

An accurate assessment of pollutants’ exposure and precise evaluation of the clinical outcomes pose two major challenges to the contemporary environmental health research. The common methods for exposure assessment are based on residential addresses and are prone to many biases. Pollution levels are defined based on monitoring stations that are sparsely distributed and frequently distanced far from residential addresses. In addition, the degree of an association between outdoor and indoor air pollution levels is not fully elucidated, making the exposure assessment all the more inaccurate. Clinical outcomes’ assessment, on the other hand, mostly relies on the access to medical records from hospital admissions and outpatients’ visits in clinics. This method differentiates by health care seeking behavior and is therefore, problematic in evaluation of an onset, duration, and severity of an outcome. In the current paper, we review a number of novel solutions aimed to mitigate the aforementioned biases. First, a hybrid satellite-based modeling approach provides daily continuous spatiotemporal estimations with improved spatial resolution of 1 × 1 km2 and 200 × 200 m2 grid, and thus allows a more accurate exposure assessment. Utilizing low-cost air pollution sensors allowing a direct measurement of indoor air pollution levels can further validate these models. Furthermore, the real temporal-spatial activity can be assessed by GPS tracking devices within the individuals’ smartphones. A widespread use of smart devices can help with obtaining objective measurements of some of the clinical outcomes such as vital signs and glucose levels. Finally, human biomonitoring can be efficiently done at a population level, providing accurate estimates of in-vivo absorbed pollutants and allowing for the evaluation of body responses, by biomarkers examination. We suggest that the adoption of these novel methods will change the research paradigm heavily relying on ecological methodology and support development of the new clinical practices preventing adverse environmental effects on human health.
Clinical Outcomes of MLC601 (NeuroAiDTM) in Traumatic Brain Injury: A Pilot Study
Authors: Al Fauzi Asra , Prihastomo Krisna , Ranuh I. , Apriawan Tedy , Wahyuhadi Joni , Parenrengi M. , Turchan Agus , Bajamal Abdul , Notobroto Hari
Published: January 2020

Background: MLC601 is a natural product formulation from Chinese medicine that is extensively studied in ischemic stroke. Traumatic brain injury (TBI) shares pathophysiological mechanisms with ischemic stroke, yet there are few studies on the use of MLC601 in treating TBI. This Indonesian pilot study aimed to investigate clinical outcomes of MLC601 for TBI. Methods: This randomized controlled trial included subjects with nonsurgical moderate TBI allocated into two groups: with and without MLC601 over three months in addition to standard TBI treatment. Clinical outcomes were measured by the Glasgow Outcome Scale (GOS) and Barthel Index (BI) observed upon discharge and at months (M) 3 and 6. Results: Thirty-two subjects were included. The MLC601 group (n = 16) had higher GOS than the control group (n = 16) at all observation timepoints, though these differences were not statistically significant (p = 0.151). The BI values indicated a significant improvement for the MLC601 group compared to the control group at M3 (47.5 vs. 35.0; p = 0.014) and at M6 (67.5 vs. 57.5; p = 0.055). No adverse effects were associated with MLC601 treatment. Conclusion: In this cohort of nonsurgical moderate TBI subjects, MLC601 showed potential for a positive effect on clinical outcome with no adverse effects.
The Influence of 30 Years Outdoor Weathering on the Durability of Hydrophobic Agents Applied on Obernkirchener Sandstones
Authors: Orlowsky Jeanette , Braun Franziska , Groh Melanie
Published: January 2020

The durability of eleven different water repellents applied on one sandstone type was studied after a long-term weathering at seven different locations in Germany. By measuring colour changes, it could be shown that the formation of black crusts, the deposition of particles and biogenic growth caused a gradual darkening as well as significant changes in total colour over time. Additionally, the water absorption behaviour was investigated with two different methods: applying a low pressure using the pipe method and capillary water absorption measurements from a wet underlay. Afterwards, the test results were analysed with four different evaluation methods: calculation of the protection degree from pipe method and capillary water absorption, determination of the velocity of water uptake during capillary water absorption and calculation of the damaged depth of the stone surface using single-sided NMR technique. The growing damaged depth leads to an increase of the water uptake velocity and to a decrease of the protection degree of the applied hydrophobing agents. Three protective agents based on isobutyltrimethoxysilane showed already after two years of outdoor weathering a clear loss of performance, which significantly increased after 30 years of exposure.
Loss of Rb1 Enhances Glycolytic Metabolism in Kras-Driven Lung Tumors In Vivo
Authors: Conroy Lindsey R. , Dougherty Susan , Kruer Traci , Metcalf Stephanie , Lorkiewicz Pawel , He Liqing , Yin Xinmin , Zhang Xiang , Arumugam Sengodagounder , Young Lyndsay E.A. , Sun Ramon C. , Clem Brian F.
Published: January 2020

Dysregulated metabolism is a hallmark of cancer cells and is driven in part by specific genetic alterations in various oncogenes or tumor suppressors. The retinoblastoma protein (pRb) is a tumor suppressor that canonically regulates cell cycle progression; however, recent studies have highlighted a functional role for pRb in controlling cellular metabolism. Here, we report that loss of the gene encoding pRb (Rb1) in a transgenic mutant Kras-driven model of lung cancer results in metabolic reprogramming. Our tracer studies using bolus dosing of [U-13C]-glucose revealed an increase in glucose carbon incorporation into select glycolytic intermediates. Consistent with this result, Rb1-depleted tumors exhibited increased expression of key glycolytic enzymes. Interestingly, loss of Rb1 did not alter mitochondrial pyruvate oxidation compared to lung tumors with intact Rb1. Additional tracer studies using [U-13C,15N]-glutamine and [U-13C]-lactate demonstrated that loss of Rb1 did not alter glutaminolysis or utilization of circulating lactate within the tricarboxylic acid cycle (TCA) in vivo. Taken together, these data suggest that the loss of Rb1 promotes a glycolytic phenotype, while not altering pyruvate oxidative metabolism or glutamine anaplerosis in Kras-driven lung tumors.
Cytoskeletal Proteins in Cancer and Intracellular Stress: A Therapeutic Perspective
Authors: Ong Mei Shan , Deng Shuo , Halim Clarissa Esmeralda , Cai Wanpei , Tan Tuan Zea , Huang Ruby Yun-Ju , Sethi Gautam , Hooi Shing Chuan , Kumar Alan Prem , Yap Celestial T.
Published: January 2020

Cytoskeletal proteins, which consist of different sub-families of proteins including microtubules, actin and intermediate filaments, are essential for survival and cellular processes in both normal as well as cancer cells. However, in cancer cells, these mechanisms can be altered to promote tumour development and progression, whereby the functions of cytoskeletal proteins are co-opted to facilitate increased migrative and invasive capabilities, proliferation, as well as resistance to cellular and environmental stresses. Herein, we discuss the cytoskeletal responses to important intracellular stresses (such as mitochondrial, endoplasmic reticulum and oxidative stresses), and delineate the consequences of these responses, including effects on oncogenic signalling. In addition, we elaborate how the cytoskeleton and its associated molecules present themselves as therapeutic targets. The potential and limitations of targeting new classes of cytoskeletal proteins are also explored, in the context of developing novel strategies that impact cancer progression.
Sensor Sensibility—HIV-1 and the Innate Immune Response
Authors: Yin Xin , Langer Simon , Zhang Zeli , Herbert Kristina M. , Yoh Sunnie , König Renate , Chanda Sumit K.
Published: January 2020

Innate immunity represents the human immune system’s first line of defense against a pathogenic intruder and is initiated by the recognition of conserved molecular structures known as pathogen-associated molecular patterns (PAMPs) by specialized cellular sensors, called pattern recognition receptors (PRRs). Human immunodeficiency virus type 1 (HIV-1) is a unique human RNA virus that causes acquired immunodeficiency syndrome (AIDS) in infected individuals. During the replication cycle, HIV-1 undergoes reverse transcription of its RNA genome and integrates the resulting DNA into the human genome. Subsequently, transcription of the integrated provirus results in production of new virions and spreading infection of the virus. Throughout the viral replication cycle, numerous nucleic acid derived PAMPs can be recognized by a diverse set of innate immune sensors in infected cells. However, HIV-1 has evolved efficient strategies to evade or counteract this immune surveillance and the downstream responses. Understanding the molecular underpinnings of the concerted actions of the innate immune system, as well as the corresponding viral evasion mechanisms during infection, is critical to understanding HIV-1 transmission and pathogenesis, and may provide important guidance for the design of appropriate adjuvant and vaccine strategies. Here, we summarize current knowledge of the molecular basis for sensing HIV-1 in human cells, including CD4+ T cells, dendritic cells, and macrophages. Furthermore, we discuss the underlying mechanisms by which innate sensing is regulated, and describe the strategies developed by HIV-1 to evade sensing and immune responses.
Acknowledgement to Reviewers of ChemEngineering in 2019
Authors: ChemEngineering Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial[...]
An Erosion-Corrosion Investigation of Coated Steel for Applications in the Oil and Gas Field, Based on Bipolar Electrochemistry
Authors: Mele Claudio , Lionetto Francesca , Bozzini Benedetto
Published: January 2020

In this research, a simple experimental apparatus based on a bipolar electrode (BPE) configuration was set up, in order to tackle erosion-corrosion problems of materials of interest in the oil and gas field. As a case study, the resistance to erosion and corrosion of carbon steel samples coated by Electroless Nickel Plating and by thermo-sprayed coating with the high velocity oxy fuel (HVOF) process was investigated. The main objective was to demonstrate if this simple, contactless technique could be applied to effectively discriminate the erosion-corrosion behavior of different materials in a vast range of experimental conditions. In fact, by means of polarization curves, visual inspection and morphological analysis by scanning electron microscope (SEM), the effects due to erosion-corrosion by solid particles, by fluid and those due to simple erosion were evaluated.
Analysis of Optical Performance Degradation in an Ion-Doped Liquid-Crystal Cell with Electrical Circuit Modeling
Authors: Seo Jeong-Ho , Huh Jae-Won , Sohn Ho-Jin , Lim Eunjung , Yoon Tae-Hoon
Published: January 2020

We reported electrical circuit modeling to analyze the optical performance degradation in an ion-doped liquid-crystal (LC) cell, which exhibited advantages, such as excellent optical performance and simple switching process, but suffered from long-term reliability issues. When an electric field was applied to the cell for an extended period of time, the optical performance became nonuniform, and the haze in the opaque state decreased. By measuring the impedance and fitting the measured data by using an equivalent circuit model, we confirmed the changes of the parameters in the electrochemical impedance spectroscopy and electrophysical properties of the ion-doped LC cell with time. According to the measurement of the optical and physical characteristics, the optical performance degradation was caused mainly by the ionic materials.
A New Species of Paraturbanella Remane, 1927 (Gastrotricha, Macrodasyida) from the Brazilian Coast, and the Molecular Phylogeny of Turbanellidae Remane, 1926
Authors: Campos Ariane , Todaro M. Antonio , Garraffoni André Rinaldo Senna
Published: January 2020

The family Turbanellidae includes Paraturbanella and five other genera. Despite the fact that the monophyly of these genera were not satisfactorily tested, species belonging to the genus Paraturbanella are distinguished from turbanellids by sharing a peculiar group of tubes on the ventrolateral side of the anterior pharyngeal region known as “dohrni” tubes. In this study, Paraturbanella tricaudata species nova (sp. nov.) from the intertidal zone of a sandy beach in Trindade (Rio de Janeiro State) and the sublittoral sand of Prumirim Island (São Paulo State), Brazil, is described. The new species can be distinguished from all other Paraturbanella species by the presence of three caudal cones (one medial and two laterals to it) and peculiar arrangement of the male system. This is the first description of a Paraturbanella species from Brazil and the third registered from the Southern Hemisphere (as opposed to 19 species in the Northern Hemisphere); thus, knowledge of marine gastrotrichs biodiversity in this region is far from satisfactory.
Quantification of Endogenous Brain Tissue Displacement Imaging by Radiofrequency Ultrasound
Authors: Jurkonis Rytis , Makūnaitė Monika , Baranauskas Mindaugas , Lukoševičius Arūnas , Sakalauskas Andrius , Matijošaitis Vaidas , Rastenytė Daiva
Published: January 2020

The purpose of this paper is a quantification of displacement parameters used in the imaging of brain tissue endogenous motion using ultrasonic radiofrequency (RF) signals. In a preclinical study, an ultrasonic diagnostic system with RF output was equipped with dedicated signal processing software and subject head–ultrasonic transducer stabilization. This allowed the use of RF scanning frames for the calculation of micrometer-range displacements, excluding sonographer-induced motions. Analysis of quantitative displacement estimates in dynamical phantom experiments showed that displacements of 55 µm down to 2 µm were quantified as confident according to Pearson correlation between signal fragments (minimum p ≤ 0.001). The same algorithm and scanning hardware were used in experiments and clinical imaging which allows translating phantom results to Alzheimer’s disease patients and healthy elderly subjects as examples. The confident quantitative displacement waveforms of six in vivo heart-cycle episodes ranged from 8 µm up to 263 µm (Pearson correlation p ≤ 0.01). Displacement time sequences showed promising possibilities to evaluate the morphology of endogenous displacement signals at each point of the scanning plane, while displacement maps—regional distribution of displacement parameters—were essential for tissue characterization.
Low-Complexity Rate-Distortion Optimization of Sampling Rate and Bit-Depth for Compressed Sensing of Images
Authors: Chen Qunlin , Chen Derong , Gong Jiulu , Ruan Jie
Published: January 2020

Compressed sensing (CS) offers a framework for image acquisition, which has excellent potential in image sampling and compression applications due to the sub-Nyquist sampling rate and low complexity. In engineering practices, the resulting CS samples are quantized by finite bits for transmission. In circumstances where the bit budget for image transmission is constrained, knowing how to choose the sampling rate and the number of bits per measurement (bit-depth) is essential for the quality of CS reconstruction. In this paper, we first present a bit-rate model that considers the compression performance of CS, quantification, and entropy coder. The bit-rate model reveals the relationship between bit rate, sampling rate, and bit-depth. Then, we propose a relative peak signal-to-noise ratio (PSNR) model for evaluating distortion, which reveals the relationship between relative PSNR, sampling rate, and bit-depth. Finally, the optimal sampling rate and bit-depth are determined based on the rate-distortion (RD) criteria with the bit-rate model and the relative PSNR model. The experimental results show that the actual bit rate obtained by the optimized sampling rate and bit-depth is very close to the target bit rate. Compared with the traditional CS coding method with a fixed sampling rate, the proposed method provides better rate-distortion performance, and the additional calculation amount amounts to less than 1%.
Eigenvalues of Two-State Quantum Walks Induced by the Hadamard Walk
Authors: Endo Shimpei , Endo Takako , Komatsu Takashi , Konno Norio
Published: January 2020

Existence of the eigenvalues of the discrete-time quantum walks is deeply related to localization of the walks. We revealed, for the first time, the distributions of the eigenvalues given by the splitted generating function method (the SGF method) of the space-inhomogeneous quantum walks in one dimension we had treated in our previous studies. Especially, we clarified the characteristic parameter dependence for the distributions of the eigenvalues with the aid of numerical simulation.
Acknowledgement to Reviewers of Entropy in 2019
Authors: Entropy Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not[...]
Is Investing in Companies Manufacturing Solar Components a Lucrative Business? A Decision Tree Based Analysis
Authors: Tomczak Sebastian Klaudiusz , Skowrońska-Szmer Anna , Szczygielski Jan Jakub
Published: January 2020

In an era of increasing energy production from renewable sources, the demand for components for renewable energy systems has dramatically increased. Consequently, managers and investors are interested in knowing whether a company associated with the semiconductor and related device manufacturing sector, especially the photovoltaic (PV) systems manufacturers, is a money-making business. We apply a new approach that extends prior research by applying decision trees (DTs) to identify ratios (i.e., indicators), which discriminate between companies within the sector that do (designated as “green”) and do not (“red”) produce elements of PV systems. Our results indicate that on the basis of selected ratios, green companies can be distinguished from the red companies without an in-depth analysis of the product portfolio. We also find that green companies, especially operating in China are characterized by lower financial performance, thus providing a negative (and unexpected) answer to the question posed in the title.
Growth and Production of Lipids in Raphidocelis subcapitata Immobilized in Sodium Alginate Beads
Authors: Benasla Amel , Hausler Robert
Published: January 2020

The growth and production of lipids in the green microalga Raphidocelis subcapitata immobilized in alginate gel are studied. The beads are made from alginate (2% w/v) and CaCl2 (1% w/v). The dry weight, the concentration of cells, and the lipid content are determined after dissolution of the beads in a sodium phosphate buffer. The results show that variations in biomass do not reflect variations in the number of cells in R. subcapitata. Cells divide more rapidly (Gc = 3.45 ± 0.3 days) than biomass is produced (Gm = 4.1 ± 0.4 days) during the exponential growth phase. Therefore, the average mass of the immobilized cells decreases until it reaches its minimum at the end of the exponential phase. Thus, during the stationary phase, cell division ceases while biomass production continues, and the average mass of the immobilized cells increases. In the present study, it is shown that this increase is due to the accumulation of lipids following the depletion of nitrates and phosphates in the culture medium. A lipid content of 24.7 ± 2.5% (dcw) and a lipid productivity of LP = 29.8 ± 3.0 mg/L/day are recorded at the end of culture. These results suggest that immobilized R. subcapitata has promising potential for biodiesel production.
Fluid–Structure Interaction Simulations of a Wind Gust Impacting on the Blades of a Large Horizontal Axis Wind Turbine
Authors: Santo Gilberto , Peeters Mathijs , Van Paepegem Wim , Degroote Joris
Published: January 2020

The effect of a wind gust impacting on the blades of a large horizontal-axis wind turbine is analyzed by means of high-fidelity fluid–structure interaction (FSI) simulations. The employed FSI model consisted of a computational fluid dynamics (CFD) model reproducing the velocity stratification of the atmospheric boundary layer (ABL) and a computational structural mechanics (CSM) model loyally reproducing the composite materials of each blade. Two different gust shapes were simulated, and for each of them, two different amplitudes were analyzed. The gusts were chosen to impact the blade when it pointed upwards and was attacked by the highest wind velocity due to the presence of the ABL. The loads and the performance of the impacted blade were studied in detail, analyzing the effect of the different gust shapes and intensities. Also, the deflections of the blade were evaluated and followed during the blade’s rotation. The flow patterns over the blade were monitored in order to assess the occurrence and impact of flow separation over the monitored quantities.
A Model of Moist Polymer Foam and a Scheme for the Calculation of Its Thermal Conductivity
Authors: Nikitsin Vadzim I. , Alsabry Abdrahman , Kofanov Valery A. , Backiel-Brzozowska Beata , Truszkiewicz Paweł
Published: January 2020

This paper proposes a method for determining an effective value of the thermal conductivity for moist, highly porous rigid polymer foams. The model of moist foam based on an ordered structure with interpenetrating components was developed in accordance with the moisture distribution in the pore space. With small moisture content, isolated water inclusions are formed, and the pore space is considered as a binary system (vapor-gas mixture and water) with isolated inclusions. With an increase in moisture content, isolated water inclusions merge, forming a continuous layer, and pore space is considered as a binary system of interpenetrating components. The thermal conductivity of the vapor-gas mixture is represented as the sum of the thermal conductivity of the dry gas and the thermal conductivity of the vapor caused by the diffusion transfer of vapor in the pore space, taking into account the coefficient of vapor diffusion resistance. Using the proposed scheme of calculation, a computational experiment was performed to establish the influence of the vapor diffusion, moisture content, and average temperature of the foam on its thermal conductivity.
Energy Performance Comparison between Two Liquid Desiccant and Evaporative Cooling-Assisted Air Conditioning Systems
Authors: Liu Su , Jeong Jae-Weon
Published: January 2020

This study investigated the annual energy saving potential and system performance of two different evaporative cooling-based liquid desiccant and evaporative cooling-assisted air conditioning systems. One system used an indirect and direct evaporative cooler with a two-stage package to match the target supply air point. The other was equipped with a single-stage, packaged dew-point evaporative cooler that used a portion of the process air, which had been dehumidified in advance. Systems installed with the two evaporative coolers were compared to determine which one was more energy efficient and which one could provide better thermal comfort for building occupants in a given climate zone, using detailed simulation data. The detailed energy consumption data of these two systems were estimated using an engineering equation solver with each component model. The results showed that the liquid desiccant and dew-point evaporative-cooler-assisted 100% outdoor air system (LDEOAS) resulted in approximately 34% more annual primary energy consumption than that of the liquid desiccant and the indirect and direct evaporative-cooler-assisted 100% outdoor air system (LDIDECOAS). However, the LDEOAS could provide drier and cooler supply air, compared with the LDIDECOAS. In conclusion, LDIDECOAS has a higher energy saving potential than LDEOAS, with an acceptable level of thermal comfort.
The Necessity of Improving the Standard for Thermal Environment in Korean Public Facilities
Authors: Jun Yong-Joon , Yoon Jin-Ha , Kim Shin , Song Young-Hak , Park Kyung-Soon
Published: January 2020

As one of the energy saving policies, the Korean government has been regulating the indoor thermal environment of public office facilities in Korea, starting with energy conservation measures in 1980. This policy, which is above 28 °C in summer and below 18 °C in winter, is causing discomfort among the occupants. The purpose of this study is to support the need to improve temperature limitation standards of the Korean public office facilities. For this purpose, the standards for the thermal environment in offices of major countries and associations were examined. Subsequently, they were compared with the Korean standards. Additionally, nine buildings of public office facilities in Korea were surveyed on the thermal environment, and PMV measurement was carried out. As a result, most of the buildings that complied with the cooling temperature standard as well as most of the buildings that did not comply were found to be uncomfortable. In conclusion, to improve the comfort of Korean public office facilities in the heating and cooling period, it is necessary to mitigate temperature regulation and regulate additional environmental factors.
Testing Non-Linear Nexus between Service Sector and CO2 Emissions in Pakistan
Authors: Hashmi Shujahat Haider , Hongzhong Fan , Fareed Zeeshan , Bannya Roksana
Published: January 2020

Our pioneer study is aimed at investigating the role of the service sector in affecting sustainable environment in Pakistan. Using time series data over 1971–2014 and applying an autoregressive distributive lag (ARDL) model with structural break analysis, we establish a long-term equilibrium relationship of carbon dioxide (CO2) emissions with energy consumption, income level, services and trade openness. Our findings support a service-induced environmental Kuznets curve (EKC) hypothesis in Pakistan. The income level sharply raises environmental degradation at the early stage; however, after reaching a certain threshold, it improves environmental quality but at a lower rate. There exists an inverted U-shaped nexus between services and CO2 emissions, which implies that the service sector is less energy-intensive in terms of mitigating pollution in Pakistan. Moreover, the energy consumption has an inverted U-shaped effect on carbon emissions, which implies energy efficiencies and adoption of renewable energy has reduced pollution in the long run. The trade openness increases CO2 emissions in both the short term and long term. The quadratic term of income level has a negatively inelastic impact on CO2 emissions, which implies a very slow rate of improvement in environmental quality. On the other hand, the quadratic term of services shows a highly elastic impact on pollution, which induces the EKC hypothesis. Our robustness checks such as fully modified ordinary least squares (FMOLS), dynamic ordinary least squares (OLS), and Toda and Yamamoto (TY) causality tests further confirm the existence of the service-induced EKC hypothesis in Pakistan. Moreover, there exists a unidirectional causality from energy consumption to CO2 emissions, a bidirectional causal relationship between economic growth and CO2 emissions, and a unidirectional causal linkage between services and CO2 emissions. Lastly, we discuss certain policy implications for designing appropriate environmental and energy policies to mitigate the pollution in Pakistan.
Experimental Investigation of the Spatial and Temporal Evolution of the Tangential and Normal E-Field Components along the Stress Grading System of a Real Stator Bar
Authors: Koné Gbah , Volat Christophe , Hudon Claude , Bernier Simon
Published: January 2020

This paper presents results based on direct experimental measurements of tangential (Et) and normal (En) E-field components along the stress grading system (SGS) of a real stator bar (Roebel type) for different AC 60 Hz applied voltages. These measurements were made with a new electro-optic system allowing for the study of both spatial distributions of two E-field components along the bar and their temporal evolution at critical points. The results obtained allowed us to calculate the correlation between the distribution of En and Et along the SGS. In particular, it was demonstrated that the En distribution presents a characteristic minimum, which can be used to identify the zone of partial discharge inception. Moreover, it was possible to observe an enlargement of the Et component distribution followed by a saturation in magnitude with the applied voltage increase. Moreover, the results have demonstrated that the waveform of the En component is mostly affected by the SG material used, producing a greater distortion in its waveform than those obtained for the Et component. The more significant distortion was obtained at the end of the outer corona protection (OCP) material, corresponding to the first maximum of the En component and characterized by the appearance of a third harmonic of large amplitude.
Fire Severity Drives the Natural Regeneration of Cytisus scoparius L. (Link) and Salix atrocinerea Brot. Communities and the Germinative Behaviour of These Species
Authors: Cruz Óscar , García-Duro Juan , F. Riveiro Sheila , García-García Candela , Casal Mercedes , Reyes Otilia
Published: January 2020

Research Highlights: Data indicated that fire severity modulates natural regeneration of Cytisus scoparius and Salix atrocinerea communities and drives much stronger effects on the germination of the dominant species. Background and Objectives: Previous studies demonstrated that fire severity induces different behaviours in plant species. Mother plant age is an important feature that must also be considered in plans of forest restoration. The objectives were to determine, in field studies, the effect of fire severity on the natural regeneration of C. scoparius and S. atrocinerea communities, to know the role of mother plant age on the germination of seeds of C. scoparius and S. atrocinerea, and to quantify their germination response at different levels of fire severity, in laboratory settings. Material and Methods: We have analysed the role of fire severity on the natural regeneration of C. scoparius and S. atrocinerea communities considering cover and height. Forty 30 × 30 m plots were randomly located in C. scoparius and S. atrocinerea communities. Fire severity on the germination of dominant species was tested through different levels of smoke, charcoal, ash, and heat. Results: High severity reduced the vertical cover and growth in height of the two communities and favoured the increase of cover of woody species in the C. scoparius community and herbaceous species in the S. atrocinerea community. Mother plant age determined germination percentages of C. scoparius seeds. Germination of C. scoparius was increased by moderate heat, and heat and smoke; and fire severity greatly reduced germination of S. atrocinerea. Conclusions: The regeneration responses after fire were largely controlled by interactions between the fire severity and the individual species regeneration strategies. For restoration purposes, C. scoparius seeds should be treated with 80 °C and smoke for 10 min, in order to increase germination; however, Salix seeds should be used without treatment and immediately after dispersion.
Why Does Phlebiopsis gigantea not Always Inhibit Root and Butt Rot in Conifers?
Authors: Żółciak Anna , Sikora Katarzyna , Wrzosek Marta , Damszel Marta , Sierota Zbigniew
Published: January 2020

This review aims to identify possible causes of differing effectiveness of artificial biological control of Heterobasidion root rot by the saprotrophic fungus Phlebiopsis gigantea. We describe published information in terms of pathogen–competitor relationships and the impact of environmental and genetic factors. We also revisit data from original research performed in recent years at the Forest Research Institute in Poland. We hypothesized that, in many cases, competition in roots and stumps of coniferous trees between the necrotrophic Heterobasidion spp. and the introduced saprotroph, Phlebiopsis gigantea, is affected by growth characteristics and enzymatic activity of the fungi, the characteristics of the wood, and environmental conditions. We concluded that both wood traits and fungal enzymatic activity during wood decay in roots and stumps, and the richness of the fungal biota, may limit biological control of root rot. In addition, we identify the need for research on new formulations and isolates of the fungal competitor, Phlebiopsis gigantea, as well as on approaches for accurately identifying the infectious threat from pathogens.
Acknowledgement to Reviewers of Future Internet in 2019
Authors: Future Internet Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not [...]
Acknowledgement to Reviewers of Fire in 2019
Authors: Fire Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not [...]
Efficacy of Lactic Acid and Modified Atmosphere Packaging against Campylobacter jejuni on Chicken during Refrigerated Storage
Authors: Gonzalez-Fandos Elena , Maya Naiara , Martínez-Laorden Alba , Perez-Arnedo Iratxe
Published: January 2020

The present study was conducted to evaluate the combined effect of lactic acid washing and modified atmospheres packaging on the counts of Campylobacter jejuni on chicken legs stored at 4 °C. In experiment 1, inoculated chicken legs were washed with either 1% or 2% lactic acid solution for 5 min or distilled water (control). The treatment with 2% lactic acid reduced C. jejuni counts 1.42 log units after treatment (day 0). In experiment 2, inoculated samples were packaged under different conditions: air, 100%N2, vacuum, 20%CO2/80%N2, or 40%CO2/60%N2. C. jejuni counts were higher in samples packaged under vacuum or atmospheres containing CO2 than in air. In experiment 3, inoculated chicken legs were washed with a 2% lactic acid solution for 5 min or distilled water (control). Samples were packaged under different conditions: air, vacuum, 20%CO2/80%N2, or 40%CO2/60%N2. C. jejuni counts were lower in samples treated with lactic acid than in samples non-treated. However, C. jejuni counts were higher in chicken legs treated with lactic acid and packaged in modified atmospheres than in those treated and packaged in air. Immersion of chicken legs in a solution containing 2% lactic acid can reduce C. jejuni counts on fresh chicken packaged in modified atmosphere.
Understanding the Relevance of DNA Methylation Changes in Immune Differentiation and Disease
Authors: Calle-Fabregat Carlos de la , Morante-Palacios Octavio , Ballestar Esteban
Published: January 2020

Immune cells are one of the most complex and diverse systems in the human organism. Such diversity implies an intricate network of different cell types and interactions that are dependently interconnected. The processes by which different cell types differentiate from progenitors, mature, and finally exert their function requires an orchestrated succession of molecular processes that determine cell phenotype and function. The acquisition of these phenotypes is highly dependent on the establishment of unique epigenetic profiles that confer identity and function on the various types of effector cells. These epigenetic mechanisms integrate microenvironmental cues into the genome to establish specific transcriptional programs. Epigenetic modifications bridge environment and genome regulation and play a role in human diseases by their ability to modulate physiological programs through external stimuli. DNA methylation is one of the most ubiquitous, stable, and widely studied epigenetic modifications. Recent technological advances have facilitated the generation of a vast amount of genome-wide DNA methylation data, providing profound insights into the roles of DNA methylation in health and disease. This review considers the relevance of DNA methylation to immune system cellular development and function, as well as the participation of DNA methylation defects in immune-mediated pathologies, illustrated by selected paradigmatic diseases.
Myosin and Other Energy-Transducing ATPases: Structural Dynamics Studied by Electron Paramagnetic Resonance
Authors: Arata Toshiaki
Published: January 2020

The objective of this article was to document the energy-transducing and regulatory interactions in supramolecular complexes such as motor, pump, and clock ATPases. The dynamics and structural features were characterized by motion and distance measurements using spin-labeling electron paramagnetic resonance (EPR) spectroscopy. In particular, we focused on myosin ATPase with actin–troponin–tropomyosin, neural kinesin ATPase with microtubule, P-type ion-motive ATPase, and cyanobacterial clock ATPase. Finally, we have described the relationships or common principles among the molecular mechanisms of various energy-transducing systems and how the large-scale thermal structural transition of flexible elements from one state to the other precedes the subsequent irreversible chemical reactions.