bluuebunny/crossref_metadata_2025 huggingface dataset:
Linear and Non-Linear Regression Analysis for the Adsorption Kinetics of SO2 in a Fixed Carbon Bed Reactor—A Case Study
Authors: Kisiela-Czajka Anna M. , Dziejarski Bartosz
Published: January 2022

Here, we determined the kinetic parameters of SO2 adsorption on unburned carbons from lignite fly ash and activated carbons based on hard coal dust. The model studies were performed using the linear and non-linear regression method for the following models: pseudo first and second order, intraparticle diffusion, and chemisorption on a heterogeneous surface. The quality of the fitting of a given model to empirical data was assessed based on: R2, R, Δq, SSE, ARE, χ2, HYBRID, MPSD, EABS, and SNE. It was clearly shown that the linear regression more accurately reflects the behaviour of the adsorption system, which is consistent with the first-order kinetic reaction—for activated carbons (SO2 + Ar) or chemisorption on a heterogeneous surface—for unburned carbons (SO2 + Ar and SO2 + Ar + H2O(g) + O2) and activated carbons (SO2 + Ar + H2O(g) + O2). Importantly, usually, each of the approaches (linear/non-linear) indicated a different mechanism of the studied phenomenon. A certain universality of the χ2 and HYBRID functions has been proved, the minimization of which repeatedly led to the lowest SNE values for the indicated models. Fitting data by any of the non-linear equations based on the R or R2 functions only cannot be treated as evidence/prerequisite of the existence of a given adsorption mechanism.
A Comparative Analysis of Brake-by-Wire Smart Actuators Using Optimization Strategies
Authors: Arasteh Ehsan , Assadian Francis
Published: January 2022

Since the automotive industry is shifting towards electrification, brake-by-wire technologies are becoming more prevalent. However, there has been little research comparing and optimizing brake-by-wire actuators in terms of their energy expenditure and response time. This paper investigates the comparison of three different smart brake-by-wire actuators, Electro-Hydraulic Brakes (EHB), Electro-Mechanical Brakes (EMB), and Electronic Wedge Brakes (EWB), first by defining an objective metric and then using both linear and nonlinear optimization techniques. Modeling of the actuators is performed using the bond graph method. Then, the controllers are designed using a robust control strategy, Youla parameterization. After designing the controllers, two types of optimization are performed on the actuators. Optimizations are performed in two ways: 1. by linearizing the plants and optimizing using their transfer functions and 2. by nonlinear optimization of the plants in the closed-loop following a specific clamp force target. The objective metrics or the cost functions for these optimizations are chosen to be the energy usage of the plants during the closed-loop operation, maximum power requirement, and their dynamic responsiveness. Using this optimization framework, we can show a significant improvement in the energy usage of the actuators and slight improvements in their responsiveness. In the end, the actuators are compared in terms of their energy usage for sets of initial and optimized physical parameters.
Comparative Thermal Performances between Pumped Thermosyphon Loops with Different Condenser Configurations Using R245fa as Working Fluid
Authors: Chang Shyy-Woei , Tsai Yn-An , Tsai Cheng-Lin
Published: January 2022

A pumped two-phase thermosyphon loop is broadly utilized to intensify the cooling duties of electronic chipsets/systems and the effectiveness for harvesting thermal energy. The configuration of a condenser not only affects the heat transfer in the condenser, but also has an effect on the saturation pressures during the boiling and condensation processes to alter the hydrothermal performance of a pumped thermosyphon loop. The influence of the condenser configuration on the hydrothermal performance of a pumped thermosyphon loop is rarely studied. The present study comparatively examined the thermal performances of two pumped thermosyphon loops with a conventional tube-fin condenser and the expansion-tank condenser. The thermodynamic cycles in pressure-temperature and pressure-enthalpy diagrams, Nusselt numbers of evaporator and condenser, thermal resistances and various performance indexes evaluated at constant pumping powers at the controlled through-flow Reynolds numbers, boiling numbers and condenser thermal resistances were measured. At the similar thermal loads, flow rates, and fluid entry temperatures of condenser, the operating pressure of the thermosyphon loop with expansion tank condenser was considerably reduced from that with tube fin condenser, leading to the lower saturation temperature for reducing the thermal resistance and the lesser pressure drop across the loop with a noticeable hydrothermal performance improvement. At the parametric conditions tested, the ratio of dimensionless overall thermal resistances between the loops with expansion tank condenser and tube fin condenser fell in the range of 0.81–0.99. When the loop performance was compared at a constant cooling airflow rate without considering the more pumping power consumption for the loop with tube fin condenser, the ranges of thermal resistance for the loops with expansion tank condenser and tube fin condenser were 0.13–0.21 (KW−1) and 0.15–0.23 (KW−1). The merit indices evaluating the comparative hydrothermal performances of evaporator, condenser and loop between the two looped thermosyphons highlighted the significance of condenser design and affirmed the performance improvement by changing tube fin condenser into expansion tank condenser. The empirical correlations of evaporator Nusselt number, condenser Nusselt number, and overall thermal resistance using Reynolds number, boiling number, and condenser thermal resistance as the controlling parameters were generated for relevant applications.
Hydrogen in Grid Balancing: The European Market Potential for Pressurized Alkaline Electrolyzers
Authors: Stamatakis Emmanuel , Perwög Ewald , Garyfallos Ermis , Millán Mercedes , Zoulias Emmanuel , Chalkiadakis Nikolaos
Published: January 2022

To limit the global temperature change to no more than 2 °C by reducing global emissions, the European Union (EU) set up a goal of a 20% improvement on energy efficiency, a 20% cut of greenhouse gas emissions, and a 20% share of energy from renewable sources by 2020 (10% share of renewable energy (RE), specifically in the transport sector). By 2030, the goal is a 27% improvement in energy efficiency, a 40% cut of greenhouse gas emissions, and a 27% share of RE. However, the integration of RE in energy system faces multiple challenges. The geographical distribution of energy supply changes significantly the availability of the primary energy source (wind, solar, water) and is the determining factor, rather than where the consumers are. This leads to an increasing demand to match supply and demand for power. Especially intermittent RE like wind and solar power face the issue of energy production unrelated to demand (issue of excess energy production beyond demand and/or grid capacity) and forecast errors leading to an increasing demand for grid services like balancing power. Megawatt electrolyzer units (beyond 3 MW) can provide a technical solution to convert large amounts of excess electricity into hydrogen for industrial applications, substitute for natural gas, or the decarbonization of the mobility sector. The demonstration of successful MW electrolyzer operation providing grid services under dynamic conditions as request by the grid can broaden the opportunities of new business models that demonstrate the profitability of an electrolyzer in these market conditions. The aim of this work is the demonstration of a technical solution utilizing Pressurized Alkaline Electrolyzer (PAE) technology for providing grid balancing services and harvesting Renewable Energy Sources (RES) under realistic circumstances. In order to identify any differences between local market and grid requirements, the work focused on a demonstration site located in Austria, deemed as a viable business case for the operation of a large-scale electrolyzer. The site is adapted to specific local conditions commonly found throughout Europe. To achieve this, this study uses a market-based solution that aims at providing value-adding services and cash inflows, stemming from the grid balancing services it provides. Moreover, the work assesses the viability of various business cases by analyzing (qualitatively and quantitatively) additional business models (in terms of business opportunities/energy source, potential grid service provision, and hydrogen demand) and analyzing the value and size of the markets developing recommendations for relevant stakeholder to decrease market barriers.
Numerical Investigation of Jet Control Using Two Pulsed Jets under Different Amplitudes
Authors: Eri Qitai , Ding Wenhao , Kong Bo
Published: January 2022

A turbulent jet with a Reynolds number of 71,000, controlled by a pair of pulsed jets operating 180° out of phase, is investigated by large-eddy simulation. The effect of the forcing amplitude on jet development and generated coherent structures is investigated through six cases: an unforced case and five cases at different amplitudes. With this forcing mode, the jet bifurcates in the far field that promotes the spread of the shear layer. It was found that the jet yields the largest bifurcation angle at medium amplitude, while the bifurcation is restrained at lower or higher amplitudes. As the amplitude increases, the streamwise vortex pair generated by the control jet is stronger and penetrates deeper into primary jet. This phenomenon reduces the inclination of vortex ring when mutual interaction between vortex ring and streamwise vortex pair occurs. Meanwhile, at higher amplitudes, the inclined vortex ring is stronger and unevenly distributed, prompting the jet bifurcation. In order to investigate the coupling effect of amplitude and frequency, different forcing frequencies were also simulated. The results demonstrated that the optimal frequency based on centerline velocity decay rate decreases with increasing amplitude; however, it eventually saturates around StD = 0.1.
Automated QFT-Based PI Tuning for Speed Control of SynRM Drive with Analytical Selection of QFT Control Specifications
Authors: Poola Rajesh , Hanamoto Tsuyoshi
Published: January 2022

The gains of PI controllers, used in the cascaded speed control of synchronous reluctance motors (SynRMs), are synthesized using quantitative feedback theory (QFT). A systematic design approach is employed to quantitatively determine the PI controller gains in terms of speed and current loops, using a mathematical model of the SynRM. Further, to make the QFT design a more transparent method, an analytical procedure using the frequency domain is attempted to design the QFT bounds as well as the initial search space of the optimization algorithm used in automatic loop shaping. The effectiveness of the proposed PI tuning method is verified with the extensive MATLAB/Simulink simulation environment. The results illustrate the supremacy of the proposed PI tuning method, in terms of control performance, over the conventional PI tuning method, using the analytical procedure.
Energy Efficiency of Transport Tasks Performed by the Air SAR System in the Baltic Sea: Case Study
Authors: Fiuk Jerzy , Chamier-Gliszczynski Norbert , Jacyna Marianna , Izdebski Mariusz
Published: January 2022

The issues discussed in this article concern the energy efficiency of transport tasks carried out by the air SAR system in the Baltic Sea. Search and rescue (SAR) are rescue operations consisting of finding people in danger, providing them with help, and delivering them to a safe place. The transport task is an element of the rescue operations carried out in the open water area. It is carried out by a given type of helicopter from a strictly defined rescue base. The aim of the article is to develop a method of selecting the base and means of transport for the transport task carried out by the air SAR system, based on the assessment of energy efficiency of a given transport task. The article proposes a selection model; parameterization of the model was carried out, indicators of energy efficiency evaluation were determined, and limitations were indicated. In practical terms, the authors’ model of selection is presented on the example of transport tasks carried out by the air SAR system in the Polish zone of responsibility in the Baltic Sea.
Linking the National Energy and Climate Plan with Municipal Spatial Planning and Supporting Sustainable Investment in Renewable Energy Sources in Austria
Authors: Geissler Susanne , Arevalo-Arizaga Abraham , Radlbauer David , Wallisch Peter
Published: January 2022

The Austrian National Energy and Climate Plan (NECP) refers to spatial planning as an important instrument to achieve 2030 targets because the technical potential of renewable energy sources (RES) are closely related to the types of land use. In Austria, land use is regulated by the spatial planning laws of the nine provinces, whereby the municipalities play an important role. It was the objective of the transFORMAT project to understand the scope for action of the municipalities with regard to promoting renewable energy use, and to understand the practical implications for renewable energy projects. To this end, the consolidated versions of spatial planning laws were analyzed and supported by a software tool (transFORMAT-Analyzer) that was developed to facilitate this process and the resulting follow-up activities. Responsible administrative departments were approached for supplementary information when deemed necessary. As a conclusion, the legal instrument (municipal ordinance), called a municipal development plan or concept, represents a long-term plan for the development of the municipality with the obligation or the option for revision under specific conditions. In theory, these revision intervals could be used to better align municipal plans with the NECP. In practice, however, significant barriers exist and opportunities for improvement have been identified, leading to recommendations on how investments in renewable energy systems can be planned more realistically and, thus, more sustainably.
Towards a Powerful Hardware-in-the-Loop System for Virtual Calibration of an Off-Road Diesel Engine
Authors: Riccio Antonio , Monzani Filippo , Landi Maurizio
Published: January 2022

A common challenge among internal combustion engine (ICE) manufacturers is shortening the development time while facing requirements and specifications that are becoming more complex and border in scope. Virtual simulation and calibration are effective instruments in the face of these demands. This article presents the development of zero-dimensional (0D)—real-time engine and exhaust after-treatment system (EAS) models and their deployment on a Virtual test bench (VTB). The models are created using a series of measurements acquired in a real test bench, carefully performed in view of ensuring the highest reliability of the models themselves. A zero-dimensional approach was chosen to guarantee that models could be run in real-time and interfaced to the real engine Electronic Control Unit (ECU). Being physically based models, they react to changes in the ECU calibration parameters. Once the models are validated, they are then integrated into a Simulink® based architecture with all the Inputs/Outputs connections to the ECU. This Simulink® model is then deployed on a Hardware in the Loop (HiL) machine for ECU testing and calibration. The results for engine and EAS performance and emissions align with both steady-state and transient measurements. Finally, two different applications of the HiL system are presented to explain the opportunities and advantages of this tool integrated within the standard engine development. Examples cited refer to altitude calibration activities and soot loading investigation on vehicle duty cycles. The cases described in this work are part of the actual development of one of the latest engines developed by Kohler Engines: the KDI 1903 TCR Stage V. The application of this methodology reveals a great potential for engine development and may become an essential tool for calibration engineers.
Performance-Based Analysis in Evaluation of Safety in Car Parks under Electric Vehicle Fire Conditions
Authors: Brzezinska Dorota , Bryant Paul
Published: January 2022

Even though electric vehicles (EV) were invented over a century ago, their popularity has grown significantly within the last 10 years due to the development of Li-ion battery technology. This evolution created an increase in the fire risk and hazards associated with this type of high-energy battery. This review focuses on lessons learned from electric vehicle fires and fire risk mitigation measures for passenger road vehicles partially or fully powered by Li-ion batteries. The paper presents EV fire risks, as well as historical car fires, published large-scale fire tests, and some proposed fire protection strategies in the aspect of electromobility safety for the future. Technical solutions for EV fire hazard mitigation are discussed, and methods of performance-based analysis and simulations for fire safety in car park evaluation are demonstrated. The Fire Dynamic Simulator (FDS) was used for the CFD simulations for the prediction of smoke dispersion and temperature distribution during an EV fire. The presented case study demonstrates how fire simulations could predict conditions for the safe evacuation of people and Fire Brigade intervention conditions in the case of an EV fire in a car park.
Maxent Modelling Predicts a Shift in Suitable Habitats of a Subtropical Evergreen Tree (Cyclobalanopsis glauca (Thunberg) Oersted) under Climate Change Scenarios in China
Authors: Zhang Lijuan , Zhu Lianqi , Li Yanhong , Zhu Wenbo , Chen Yingyong
Published: January 2022

Climate change has caused substantial shifts in the geographical distribution of many species. There is growing evidence that many species are migrating in response to climate change. Changes in the distribution of dominant tree species induced by climate change can have an impact not only on organisms such as epiphytes and understory vegetation, but also on the whole ecosystem. Cyclobalanopsis glauca is a dominant tree species in the mingled evergreen and deciduous broadleaf forests of China. Understanding their adaptive strategies against climate change is important for understanding the future community structure. We employed the Maxent framework to model current suitable habitats of C. glauca under current climate conditions and predicted it onto the climate scenarios for 2041–2060 and 2081–2100 using 315 occurrence data. Our results showed that annual precipitation was the most critical factor for the distribution of C. glauca. In the future, increasing precipitation would reduce the limitation of water on habitats, leading to an expansion of the distribution to a higher latitude and higher altitude. At the same time, there were habitat contractions at the junction of the Jiangxi and Fujian Provinces. This study can provide vital information for the management of C. glauca, and serve as a reminder for managers to protect C. glauca in the range contraction areas.
Assessing the Economic Contribution of Forest Use to Rural Livelihoods in the Rubi-Tele Hunting Domain, DR Congo
Authors: Mendako Richard K. , Tian Gang , Ullah Saif , Sagali Heri Labani , Kipute Daddy D.
Published: January 2022

Forest utilization makes a significant economic contribution to the livelihoods of rural households, especially those living in developing countries. This study was conducted to determine the absolute and relative forest income and measure the distributional impact of forest income on economic inequalities among rural households in the Rubi-Tele Hunting Domain (RTHD), Democratic Republic of the Congo (DR Congo). Household socioeconomic data and other qualitative informations were collected using structured household-level surveys, focus group discussions, and key informant interviews. The study used descriptive statistical analysis, Gini coefficient, and Lorenz curve computation. Additionally, the non-parametric alternative Kruskal–Wallis Test was also used. Findings revealed that 89.76% of the households’ sample were involved in forest-based activities. The annual average income from these forest-based activities was estimated at CDF 1,219,951.58 (USD 659.08) per household, contributing 32.46% to total annual household income. Other income sources share constituted 37.09%, 10.04%, 8.30%, 7.63%, 2.41%, and 2.08% from crop, livestock keeping, business activities, fishing, and non-forest environmental activities, paid employment, and other income sources, respectively. There was a significant difference between annual household income in terms of income sources (p < 0.5). Forest income constituted the second most crucial income portfolio after crop income. Bushmeat income share was higher than other forest products income share. However, the proportion of households involved in firewood extraction was substantial (80.70%). Gini coefficient and Lorenz curve analysis proved that forest income helped reduce economic inequality among the sampled households by 11%. Therefore, reducing access to forest would significantly impact rural households’ welfare and could increase income disparities. The present study provides valuable information for developing sustainable forest management policies and strategies to maintain and enhance the economic benefit of forest use without damaging biodiversity conservation.
Anatomical, Physical, Chemical, and Biological Durability Properties of Two Rattan Species of Different Diameter Classes
Authors: Ahmed Sheikh Ali , Hosseinpourpia Reza , Brischke Christian , Adamopoulos Stergios
Published: January 2022

Rattan cane is an important forest product with economic value. Its anatomical, physical, and biological properties vary with the cane height. This makes it difficult to select the appropriate cane diameter for harvesting. Understanding the material properties of rattan cane with different diameter sizes is important to enhance its utilization and performance for different end uses. Thus, the present study was performed on two rattan species, Calamus zollingeri and Calamus ornatus, at two different cane heights (bottom/mature and top/juvenile). Calamus zollingeri was studied at diameter classes of 20 mm and 30 mm, while Calamus ornatus was analyzed at a diameter class of 15 mm. The anatomical properties, basic density, volumetric swelling, dynamic moisture sorption, and biological durability of rattan samples were studied. The results showed that C. zollingeri with a 20 mm diameter exhibited the highest basic density, hydrophobicity, dimensional stability, and durability against mold and white-rot (Trametes versicolor) fungi. As confirmed by anatomical studies, this could be due to the higher vascular bundle frequency and longer thick-walled fibers that led to a denser structure than in the other categories. In addition, the lignin content might have a positive effect on the mass loss of different rattan canes caused by white-rot decay.
The Influence of Traditional and Immobilized Yeast on the Amino-Acid Content of Sparkling Wine
Authors: Prokes Kamil , Baron Mojmir , Mlcek Jiri , Jurikova Tunde , Adamkova Anna , Ercisli Sezai , Sochor Jiri
Published: January 2022

This article focuses on the effect of yeast strains, vintage, and must sugar content on the amino-acid content of sparkling wines produced by the traditional method. In the experiment, the amino-acid concentrations before and after secondary fermentation, depending on the type of yeast used (basic wine without secondary fermentation, wine fermented with immobilized yeast, and wine fermented with classical Champagne yeast) and the sugar content of the must (170, 190, and 210 g per liter), and the vintage (2010 and 2011), were evaluated. Concentrations of 20 free amino acids in 18 wine variants were analyzed by ion-exchange liquid chromatography with postcolony ninhydrin derivatization and photometric detection. Results of the study show an increase in all the amino acids represented, except ornithine, after secondary fermentation. The average content of each amino acid in the basic wine, wine fermented with immobilized yeast, and wine fermented with classical Champagne yeast was higher in the variant where classical yeast was used. In this variant, the concentrations of alanine, glutamic acid, lysine, arginine, phenylalanine, valine, and glycine were almost twice as high as in the other variants. A higher proportion of most amino acids was observed in the year 2011; only for amino acids lysine, leucine, phenylalanine, tyrosine, ornithine, histidine, and methionine was a higher concentration observed in the year 2010. A higher concentration of released amino acids was also observed in wine produced from must with a higher sugar content (21° NM).
Experimentation Environments for Data Center Routing Protocols: A Comprehensive Review
Authors: Alberro Leonardo , Castro Alberto , Grampin Eduardo
Published: January 2022

The Internet architecture has been undergoing a significant refactoring, where the past preeminence of transit providers has been replaced by content providers, which have a ubiquitous presence throughout the world, seeking to improve the user experience, bringing content closer to its final recipients. This restructuring is materialized in the emergence of Massive Scale Data Centers (MSDC) worldwide, which allows the implementation of the Cloud Computing concept. MSDC usually deploy Fat-Tree topologies, with constant bisection bandwidth among servers and multi-path routing. To take full advantage of such characteristics, specific routing protocols are needed. Multi-path routing also calls for revision of transport protocols and forwarding policies, also affected by specific MSDC applications’ traffic characteristics. Experimenting over these infrastructures is prohibitively expensive, and therefore, scalable and realistic experimentation environments are needed to research and test solutions for MSDC. In this paper, we review several environments, both single-host and distributed, which permit analyzing the pros and cons of different solutions.
Formation of Natural Egg Yolk Granule Stabilized Pickering High Internal Phase Emulsions by Means of NaCl Ionic Strength and pH Change
Authors: Mi Sijie , Xia Minquan , Zhang Xinyue , Liu Jihong , Cai Zhaoxia
Published: January 2022

Pickering high internal phase emulsions (HIPEs) are gel-like concentrated emulsions that have the potential to be an alternative to partially hydrogenated oil (PHO). In this study, egg yolk granules (EYGs), natural complexes of protein and lipid isolated from egg yolk, were used as an emulsifier to prepare Pickering HIPEs. Gel-like HIPEs with an oil phase volume fraction of 85% and with an emulsifier concentration of only 0.5% could be prepared by using EYGs as an emulsifier. The EYGs were able to form stable HIPEs at NaCl ionic strengths over 0.2 M and at pH over 5.0 with NaCl ionic strength of 0.3 M. The EYGs, which could stabilize HIPEs, were easily to adsorb and cover the oil-water interface to form emulsion droplets with small particle size. In addition, interacting EYGs in the aqueous phase formed a continuous network structure, and the oil droplets packed closely, exhibiting high elasticity and shear thinning behavior. Furthermore, the formed HIPEs had suitable storage stability with no significant changes in appearance and microstructure after storage for 60 days. This work can transform traditional oils from liquid-like to solid-like by using EYGs to enrich food processing diversity and improve the storage stability of oils while reducing the intake of PHO and providing a healthier diet for consumers.
Limosilactobacillus fermentum MG4295 Improves Hyperglycemia in High-Fat Diet-Induced Mice
Authors: Kim Ji Eun , Lee Ji Yeon , Kang Chang-Ho
Published: January 2022

Hyperglycemia due to uncontrolled glucose regulation is widely known as cause of diabetes, non-alcoholic fatty liver disease (NAFLD), and other complications. NAFLD refers to a condition in which fat is excessively accumulated, whether inflamed or not, and has caused serious medical problems in recent years. The aim of this study was to explore the antihyperglycemia effects of Limosilactobacillus fermentum MG4295 (L. fermentum MG4295) in high-fat diet (HFD)-induced in vivo. We demonstrated the suitability of L. fermentum MG4295 as a probiotic by observing its stability, survivability, and proliferation under simulated gastrointestinal conditions, and safety, antibiotic susceptibility, hemolysis, and enzyme activity. The potential antihyperglycemic activity of L. fermentum MG4295 was investigated in an HFD and sugar-water-induced mouse model. Administration of this strain for 12 weeks showed an improved trend in glucose tolerance, insulin, alanine amino transferase, total cholesterol, low-density lipoprotein cholesterol, and glucagon-like peptide-1. Histopathological analysis revealed that L. fermentum MG4295 significantly reduced the histopathological scores of hepatic steatosis, inflammation, and hepatocellular hypertrophy in liver tissues and lipid content in adipose tissues. Administration of L. fermentum MG4295 upregulated IRS-1, AKT, and GLUT4 and downregulated G6Pc and PEPCK expression in liver and/or muscle tissues. Our results suggest that L. fermentum MG4295 can improve hyperglycemia. Furthermore, it can be used as a dietary functional supplement to manage blood glucose.
Probiotic Potential of a Folate-Producing Strain Latilactobacillus sakei LZ217 and Its Modulation Effects on Human Gut Microbiota
Authors: Liu Manman , Chen Qingqing , Sun Yalian , Zeng Lingzhou , Wu Hongchen , Gu Qing , Li Ping
Published: January 2022

Folate is a B-vitamin required for DNA synthesis, methylation, and cellular division, whose deficiencies are associated with various disorders and diseases. Currently, most folic acid used for fortification is synthesized chemically, causing undesirable side effects. However, using folate-producing probiotics is a viable option, which fortify folate in situ and regulate intestinal microbiota. In this study, the folate production potential of newly isolated strains from raw milk was analyzed by microbiological assay. Latilactobacillus sakei LZ217 showed the highest folate production in Folic Acid Assay Broth, 239.70 ± 0.03 ng/μL. The folate produced by LZ217 was identified as 5-methyltetrahydrofolate. LZ217 was tolerant to environmental stresses (temperature, pH, NaCl, and ethanol), and was resistant to gastrointestinal juices. Additionally, the in vitro effects of LZ217 on human gut microbiota were investigated by fecal slurry cultures. 16S rDNA gene sequencing indicated that fermented samples containing LZ217 significantly increased the abundance of phylum Firmicutes and genus Lactobacillus, Faecalibacterium, Ruminococcus 2, Butyricicoccus compared to not containing. Short-chain fatty acids (SCFAs) analysis revealed that LZ217 also increased the production of butyric acid by fermentation. Together, L. sakei LZ217 could be considered as a probiotic candidate to fortify folate and regulate intestinal microecology.
Effects of Superheated Steam Treatment on the Allergenicity and Structure of Chicken Egg Ovomucoid
Authors: Wen Ping-Wei , Tu Zong-Cai , Hu Yue-Ming , Wang Hui
Published: January 2022

The aim of this study was to explore the effects of an emerging and efficient heating technology, superheated steam (SS), on the allergenicity and molecular structure of ovomucoid (OVM). OVM was treated with 120–200 °C of SS for 2 to 10 min. The allergenicity (IgG/IgE binding abilities and cell degranulation assay) and molecular structure (main functional groups and amino acids modification) changes were investigated. The IgG-binding ability of OVM decreased and the releases of β-hex and TNF-γ were inhibited after SS treatment, indicating that the protein allergenicity was reduced. Significant increases in oxidation degree, free SH content and surface hydrophobicity were observed in SS-treated OVM. The protein dimer and trimer appeared after SS treatment. Meanwhile, obvious changes occurred in the primary structure. Specifically, serine can be readily modified by obtaining functional groups from other modification sites during SS treatment. Moreover, the natural OVM structure which showed resistance to trypsin digestion was disrupted, leading to increased protein digestibility. In conclusion, SS-induced OVM aggregation, functional groups and amino acids modifications as well as protein structure alteration led to reduced allergenicity and increased digestibility.
Nutrition-Related Content on Instagram in the United States of America: Analytical Cross-Sectional Study
Authors: Tricas-Vidal Hector José , Vidal-Peracho María Concepción , Lucha-López María Orosia , Hidalgo-García César , Lucha-López Ana Carmen , Monti-Ballano Sofía , Corral-de Toro Jaime , Márquez-Gonzalvo Sergio , Tricás-Moreno José Miguel
Published: January 2022

Background: The Internet is today the largest platform for food distribution, and there are concerns about the impact that digital marketing has in the field of nutrition by promoting non-evidence-based recommendations. The purpose of this study was to describe the user profile that draws on Instagram to follow nutrition-related content versus not, and to analyze the frequency and type of content of the information provided by nutritional influencers. Methods: A cross-sectional study involving randomly selected United States residents having an Instagram account was performed. Participants completed an anonymous online survey link. Results: From 898 respondents, 78.7% were women, and 75.6% were Millennials. Scientific evidence present in the posts was 14.3%. Influencers promoted a product or a brand in more than 90% of posts. Women followed more nutrition-related content than men (p < 0.001). Millennials, followed by Generation-Z, followed more nutrition-related content (p < 0.001). There were no significant relationships between the following status of nutrition-related content with BMI, type of job, household income, education degree, or smoking habits. Conclusions: Women and Millennials followed more nutrition-related content. Scientific evidence was scarce and commercial interest in the network was evident. The vast majority of the posts were not based on scientific evidence and instead promoted a product/supplement.
Biodegradation of PLA-PHBV Blend Films as Affected by the Incorporation of Different Phenolic Acids
Authors: Hernández-García Eva , Vargas Maria , Chiralt Amparo , González-Martínez Chelo
Published: January 2022

Films based on a 75:25 polylactic acid (PLA) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) blend, containing 2% (w/w) of different phenolic acids (ferulic, p-coumaric or protocatechuic acid), and plasticised with 15 wt. % polyethylene glycol (PEG 1000), were obtained by melt blending and compression moulding. The disintegration and biodegradation of the film under thermophilic composting conditions was studied throughout 35 and 45 days, respectively, in order to analyse the effect of the incorporation of the antimicrobial phenolic acids into the films. Sample mass loss, thermo-degradation behaviour and visual appearance were analysed at different times of the composting period. No effect of phenolic acids was observed on the film disintegration pattern, and the films were completely disintegrated at the end of the composting period. The biodegradation analysis through the CO2 measurements revealed that PLA-PHBV blend films without phenolic acids, and with ferulic acid, completely biodegraded after 20 composting days, while p-coumaric and protocatechuic slightly retarded full biodegradation (21 and 26 days, respectively). Phenolic acids mainly extended the induction period, especially protocatechuic acid. PLA-PHBV blend films with potential antimicrobial activity could be used to preserve fresh foodstuff susceptible to microbial spoilage, with their biodegradation under composting conditions being ensured.
Big Rip Scenario in Brans-Dicke Theory
Authors: Pradhan Sasmita Kumari , Tripathy Sunil Kumar , Naik Zashmir , Behera Dipanjali , Bhuyan Mrutunjaya
Published: January 2022

In this work, we present a Big Rip scenario within the framework of the generalized Brans-Dicke (GBD) theory. In the GBD theory, we consider an evolving BD parameter along with a self-interacting potential. An anisotropic background is considered to have a more general view of the cosmic expansion. The GBD theory with a cosmological constant is presented as an effective cosmic fluid within general relativity which favours a phantom field dominated phase. The model parameters are constrained so that the model provides reasonable estimates of the Hubble parameter and other recent observational aspects at the present epoch. The dynamical aspects of the BD parameter and the BD scalar field have been analysed. It is found that the present model witnesses a finite time doomsday at a time of tBR≃16.14Gyr, and for this scenario, the model requires a large negative value of the Brans-Dicke parameter.
A Look-Up Table Based Fractional Order Composite Controller Synthesis Method for the PMSM Speed Servo System
Authors: Zheng Weijia , Huang Runquan , Luo Ying , Chen YangQuan , Wang Xiaohong , Chen Yong
Published: January 2022

Considering the performance requirements in actual applications, a look-up table based fractional order composite control scheme for the permanent magnet synchronous motor speed servo system is proposed. Firstly, an extended state observer based compensation scheme was adopted to suppress the motor parametric uncertainties and convert the speed servo plant into a double-integrator model. Then, a fractional order proportional-derivative (PDμ) controller was adopted as the speed controller to provide the optimal step response performance for the servo system. A universal look-up table was established to estimate the derivative order of the PDμ controller, according to the optimal samples collected by an improved differential evolution algorithm. With the look-up table, the optimal PDμ controller can be tuned analytically. Simulation and experimental results show that the servo system using the composite control scheme can achieve optimal tracking performance and has robustness to the motor parametric uncertainties and disturbance torques.
Third Hankel Determinant for a Subclass of Univalent Functions Associated with Lemniscate of Bernoulli
Authors: Ullah Najeeb , Ali Irfan , Hussain Sardar Muhammad , Ro Jong-Suk , Khan Nazar , Khan Bilal
Published: January 2022

This paper deals with a new subclass of univalent function associated with the right half of the lemniscate of Bernoulli. We find the upper bound of the Hankel determinant H3(1) for this subclass by applying the Carlson–Shaffer operator to it. The present work also deals with certain properties of this newly defined subclass, such as the upper bound of the Hankel determinant of order 3, coefficient estimates, etc.
Lorentz Violation in Astroparticles and Gravitational Waves
Authors: Schreck Marco
Published: January 2022

Lorentz invariance is one of the fundamental continuous symmetries of the laws of nature [...]
All Ways Lead to Rome—Meiotic Stabilization Can Take Many Routes in Nascent Polyploid Plants
Authors: Gonzalo Adrián
Published: January 2022

Newly formed polyploids often show extensive meiotic defects, resulting in aneuploid gametes, and thus reduced fertility. However, while many neopolyploids are meiotically unstable, polyploid lineages that survive in nature are generally stable and fertile; thus, those lineages that survive are those that are able to overcome these challenges. Several genes that promote polyploid stabilization are now known in plants, allowing speculation on the evolutionary origin of these meiotic adjustments. Here, I discuss results that show that meiotic stability can be achieved through the differentiation of certain alleles of certain genes between ploidies. These alleles, at least sometimes, seem to arise by novel mutation, while standing variation in either ancestral diploids or related polyploids, from which alleles can introgress, may also contribute. Growing evidence also suggests that the coevolution of multiple interacting genes has contributed to polyploid stabilization, and in allopolyploids, the return of duplicated genes to single copies (genome fractionation) may also play a role in meiotic stabilization. There is also some evidence that epigenetic regulation may be important, which can help explain why some polyploid lineages can partly stabilize quite rapidly.
Inorganic Nitrogen Transport and Assimilation in Pea (Pisum sativum)
Authors: Gu Benguo , Chen Yi , Xie Fang , Murray Jeremy D. , Miller Anthony J.
Published: January 2022

The genome sequences of several legume species are now available allowing the comparison of the nitrogen (N) transporter inventories with non-legume species. A survey of the genes encoding inorganic N transporters and the sensing and assimilatory families in pea, revealed similar numbers of genes encoding the primary N assimilatory enzymes to those in other types of plants. Interestingly, we find that pea and Medicago truncatula have fewer members of the NRT2 nitrate transporter family. We suggest that this difference may result from a decreased dependency on soil nitrate acquisition, as legumes have the capacity to derive N from a symbiotic relationship with diazotrophs. Comparison with M. truncatula, indicates that only one of three NRT2s in pea is likely to be functional, possibly indicating less N uptake before nodule formation and N-fixation starts. Pea seeds are large, containing generous amounts of N-rich storage proteins providing a reserve that helps seedling establishment and this may also explain why fewer high affinity nitrate transporters are required. The capacity for nitrate accumulation in the vacuole is another component of assimilation, as it can provide a storage reservoir that supplies the plant when soil N is depleted. Comparing published pea tissue nitrate concentrations with other plants, we find that there is less accumulation of nitrate, even in non-nodulated plants, and that suggests a lower capacity for vacuolar storage. The long-distance transported form of organic N in the phloem is known to be specialized in legumes, with increased amounts of organic N molecules transported, like ureides, allantoin, asparagine and amides in pea. We suggest that, in general, the lower tissue and phloem nitrate levels compared with non-legumes may also result in less requirement for high affinity nitrate transporters. The pattern of N transporter and assimilatory enzyme distribution in pea is discussed and compared with non-legumes with the aim of identifying future breeding targets.
Selection and Validation of Reliable Reference Genes for Gene Expression Studies in Different Genotypes and TRV-Infected Fruits of Peach (Prunus persica L. Batsch) during Ripening
Authors: Xu Ze , Dai Jieyu , Su Weijing , Wu Haixia , Shah Kamran , Xing Libo , Ma Juanjuan , Zhang Dong , Zhao Caiping
Published: January 2022

Real-time quantitative PCR (RT-qPCR) is a powerful tool to detect and quantify transcription abundance, and the stability of the reference gene determines its success. However, the most suitable reference gene for different genotypes and tobacco rattle virus (TRV) infected fruits was unclear in peach (Prunus persica L. Batsch). In this study, 10 reference genes were selected and gene expression was characterized by RT-qPCR across all samples, including different genotypes and TRV-infected fruits during ripening. Four statistical algorithms (geNorm, NormFinder, BestKeeper, and RefFinder) were used to calculate the stability of 10 reference genes. The geNorm analysis indicated that two suitable reference genes should be used for gene expression normalization. In general, the best combination of reference genes was CYP2 and Tua5 for TRV-infected fruits and CYP2 and Tub1 for different genotypes. In 18S, GADPH, and TEF2, there is an unacceptable variability of gene expression in all experimental conditions. Furthermore, to confirm the validity of the reference genes, the expression levels of PpACO1, PpEIN2, and PpPL were normalized at different fruit storage periods. In summary, our results provide guidelines for selecting reliable reference genes in different genotypes and TRV-infected fruits and lay the foundation for accurate evaluation of gene expression for RT-qPCR analysis in peach.
Genetic Rescue of the Highly Inbred Norwegian Lundehund
Authors: Melis Claudia , Pertoldi Cino , Ludington William Basil , Beuchat Carol , Qvigstad Gunnar , Stronen Astrid Vik
Published: January 2022

Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norwegian Buhund. Conservation breeding decisions for the (typically) small number of outcrossed individuals are vital for managing the rescue process, and we genotyped the Lundehund (n = 12), the Buhund (n = 12), their crosses (F1, n = 7) and first-generation backcrosses to the Lundehund (F2, n = 12) with >170,000 single nucleotide polymorphism loci to compare their levels of genetic diversity. We predicted that genome-wide diversity in F2 dogs would be higher than in the Lundehund but lower than in the F1 and the Buhund, and the heterozygosity values showed the expected patterns. We also found that runs of homozygosity, extended chromosomal regions of homozygous genotypes inherited from a common ancestor, were reduced in F2 individuals compared with Lundehund individuals. Our analyses demonstrate the benefits of outcrossing but indicate that some of the acquired genetic diversity is lost following immediate backcrossing. Additional breeding among F2 crosses could therefore merit from further consideration in genetic rescue management.
Clinical Study on the Efficacy and Safety of Arginine Administered Orally in Association with Other Active Ingredients for the Prevention and Treatment of Sarcopenia in Patients with COVID-19-Related Pneumonia, Hospitalized in a Sub-Intensive Care Unit
Authors: Bologna Carolina , Pone Eduardo
Published: January 2022

In order to evaluate the efficacy of oral supplementation with 3 g of arginine per day associated with creatine, L-carnitine, aspartic acid, magnesium, selenium and vitamins C and E (Argivit© Aesculapius Farmaceutici) in the prevention and treatment of sarcopenia in patients with COVID-19-related pneumonia, we conducted a parallel randomized study comparing it with standard therapy alone. Forty patients on standard therapy plus supplementation were compared with a control group of 40 patients, all hospitalized at the sub-intensive care unit of the Del Mare Hospital in Naples, with a clinical diagnosis of SARS-CoV-2 infection and COVID-19 pneumonia. Muscle strength was assessed with the handgrip test and muscle ultrasound. Arginine-supplemented patients had an average grip strength of 23.5 at the end of hospitalization compared with 22.5 in the untreated group with less reduction, showing statistical significance (p < 0.001). In the same way, the thickness of the vastus lateralis quadriceps femoris muscle measured at the end of hospitalization showed less reduction on ultrasound, with a higher average value in the group receiving treatment than in the group of patients without supplementation (p < 0.001). Upon discharge there was a 58.40% reduction in ventilation days in patients with arginine supplementation compared with the control group.