bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolution of Urothelial Bladder Cancer in the Context of Molecular Classifications
Authors: Minoli Martina , Kiener Mirjam , Thalmann George N. , Kruithof-de Julio Marianna , Seiler Roland
Published: August 2020

Bladder cancer is a heterogeneous disease that is not depicted by current classification systems. It was originally classified into non-muscle invasive and muscle invasive. However, clinically and genetically variable tumors are summarized within both classes. A definition of three groups may better account for the divergence in prognosis and probably also choice of treatment. The first group represents mostly non-invasive tumors that reoccur but do not progress. Contrarily, the second group represent non-muscle invasive tumors that likely progress to the third group, the muscle invasive tumors. High throughput tumor profiling improved our understanding of the biology of bladder cancer. It allows the identification of molecular subtypes, at least three for non-muscle invasive bladder cancer (Class I, Class II and Class III) and six for muscle-invasive bladder cancer (luminal papillary, luminal non-specified, luminal unstable, stroma-rich, basal/squamous and neuroendocrine-like) with distinct clinical and molecular phenotypes. Molecular subtypes can be potentially used to predict the response to treatment (e.g., neoadjuvant chemotherapy and immune checkpoint inhibitors). Moreover, they may allow to characterize the evolution of bladder cancer through different pathways. However, to move towards precision medicine, the understanding of the biological meaning of these molecular subtypes and differences in the composition of cell subpopulations will be mandatory.
Individual-Level Comparisons of Honey Bee (Hymenoptera: Apoidea) Hygienic Behavior Towards Brood Infested with Varroa destructor (Parasitiformes: Varroidae) or Tropilaelaps mercedesae (Mesostigmata: Laelapidae)
Authors: Shrestha Monica , Wegener Jakob , Gautam Ishan , Singh Madhusudan , Schwekendiek Christoph , Bienefeld Kaspar
Published: August 2020

The mites Varroa destructor Anderson and Trueman and Tropilaelaps mercedesae Anderson and Morgan are both serious threats to the Apis mellifera beekeeping industry. A trait frequently used in selection programs for V. destructor resistance is hygienic behavior, the selective removal of diseased/damaged brood. Here, we measured the level of association of the expression of hygienic behavior against both mites in A. mellifera, by observing whether the same individual bees would carry out the opening and removal of brood infested by the two parasites. The groups of bees showing these behaviors on cells artificially infested by either parasite showed a large overlap, making it appear likely that the two traits are at least closely coupled. Therefore, breeding for V. destructor resistance based on hygienic behavior could prepare A. mellifera populations for dealing with Tropilaelaps sp. mites, and vice versa. Using the same bioassay, we also compared the hygienic behavior of A. mellifera towards T. mercedesae to that of the Asiatic honey bee, Apis cerana. A. cerana workers eliminated a greater proportion of infested cells, which may in part explain the resistance of this bee to Tropilaelaps and the observation that Tropilaelaps reproduction on brood of this species is extremely rare.
Effects of a Supervised Nordic Walking Program on Obese Adults with and without Type 2 Diabetes: The C.U.R.I.A.Mo. Centre Experience
Authors: Pippi Roberto , Di Blasio Andrea , Aiello Cristina , Fanelli Carmine , Bullo Valentina , Gobbo Stefano , Cugusi Lucia , Bergamin Marco
Published: August 2020

Exercise is a convenient non-medical intervention, commonly recommended in metabolic syndrome and type 2 diabetes (DM2) managements. Aerobic exercise and aerobic circuit training have been shown to be able to reduce the risk of developing DM2-related complications. Growing literature proves the usefulness of Nordic walking as exercise therapy in different disease populations, therefore it has a conceivable use in DM2 management. Aims of this study were to analyze and report the effects of two different supervised exercises (gym-based exercise and Nordic walking) on anthropometric profile, blood pressure values, blood chemistry and fitness variables in obese individuals with and without DM2. In this study, 108 obese adults (aged 45–65 years), with or without DM2, were recruited and allocated into one of four subgroups: (1) Gym-based exercise program (n = 49) or (2) Nordic walking program (n = 37) for obese adults; (3) Gym-based exercise program (n = 10) or (4) Nordic walking program (n = 12) for obese adults with DM2. In all exercise subgroups, statistically significant improvements in body weight, body mass index, fat mass index, muscular flexibility and maximal oxygen uptake (VO2 max) were observed. Moreover, a higher percentage of adherence to the gym-based program compared to Nordic walking was recorded. Our findings showed that, notwithstanding the lower adherence, a supervised Nordic walk is effective as a conventional gym-based program to improve body weight control, body composition parameters, muscular flexibility and VO2 max levels in obese adults with and without type 2 diabetes.
Market Volatility and Investors’ View of Firm-Level Risk: A Case of Green Firms
Authors: Kyaw Khine
Published: August 2020

Do investors believe that firm-level (i.e., idiosyncratic) risk of green (i.e., environmentally responsible) firms is relatively lower? How does high market volatility affect the investors’ view on the firm-level risk of green firms? This paper addresses these questions by investigating the relationship between firm-level (idiosyncratic) risk and firms’ environmental performance. Further, we examine the effect market volatility has on the relationship. We estimate fixed-effect panel models using 8036 firm-year observations across 793 firms. We test robustness of the results with difference-in-difference (DiD), propensity score matching (PSM) and dynamic panel with the generalized method of moments (GMM) estimations. We find that investors generally associate firms that perform well on the environmental front to be of lower risk. However, during periods of high market volatility, just performing better than the industry does not make the investors see the firms’ risk as being significantly lower. How well the firms perform in relation to the industry performance is associated with the investors believing that the firm’s risk is significantly lower.
Cultural Memories and Sense of Place in Historic Urban Landscapes: The Case of Masrah Al Salam, the Demolished Theatre Context in Alexandria, Egypt
Authors: Hussein Fatmaelzahraa , Stephens John , Tiwari Reena
Published: August 2020

Historic urban landscapes (HULs) are composed of layers of history and memories that are embedded in physical monuments, buildings, and memorials. Physical built fabric stores both personal and cultural memory through long association with communities. Rapid changes due to demolition and redevelopment change the nature of these places and, in turn, affect these memory storages. This paper investigates whether historical city inhabitants consider cultural memories important when managing their HULs. It further explores the effectiveness of cultural memory in creating a sense of place and enhancing the quality of life for inhabitants. The context of the demolished theatre ‘Masrah Al Salam’ in Alexandria, Egypt, was studied after city inhabitants angrily protested the theatre’s removal, indicating a strong community attachment to this lost place. A qualitative methodological approach to this study was applied by conducting on-site, semi-structured, face-to-face interviews supplemented by comments gathered from the Facebook group ‘Alexandria’s Spirit’. The QSR NVivo12 program was used as a qualitative tool for data management, analysis, and mapping intangible elements contributing to an assembly of cultural memories of this place. The study demonstrated the importance of cultural memory associated with urban elements such as iconic heritage buildings that create a sense of place and enhance the identity of our urban environments.
Does the Introduction of Alien Species Represent a Sanitary Threat for Native Species? The Case of the Eastern Cottontail Sylvilagus floridanus in Italy
Authors: Tizzani Paolo , Andrade Daniela , Molinar Min Anna Rita , Peano Andrea , Meneguz Pier Giuseppe
Published: August 2020

Introduction of alien species is a well-known threat to biodiversity. Where newly introduced, alien species may pose a risk for the local ecological community by competing for resources or by introducing pathogens. Sylvilagus floridanus is an American lagomorph introduced into Europe in the second half of 20th century, for hunting. This study evaluated the structure and epidemiological characteristics of the gastrointestinal parasite community in an introduced population of S. floridanus in the Province of Alessandria (Piedmont Region—Italy). Three alien parasites were reported out of 271 animals: Obeliscoides cuniculi in the stomach, Trichostrongylus calcaratus in the small intestine, and Passarulus nonnanulatus in the large intestine. All these nematodes are commonly reported in S. floridanus in its natural range, but they represent alien species in Europe. The report of these alien parasites is an example of the unexpected consequences caused by the introduction of non-native vertebrates. The documented introduction of new pathogens may alter the parasite community of the native lagomorphs, with possible long-term effects on local ecological dynamics.
Electrochemical Deposition of Ni, NiCo Alloy and NiCo–Ceramic Composite Coatings—A Critical Review
Authors: Mbugua Nyambura Samuel , Kang Min , Zhang Yin , Ndiithi Ndumia Joseph , V. Bertrand Gbenontin , Yao Liang
Published: August 2020

In recent years, alloy and alloy-ceramic coatings have gained a considerable attention owing to their favorable physicochemical and technological properties. In this review, we investigate Ni, NiCo alloy and NiCo–ceramic composite coatings prepared by electrodeposition. Electrodeposition is a versatile tool and cost-effective electrochemical method used to produce high quality metal coatings. Surface finish and tribological properties of the coatings can be further improved by the addition of suitable agents and control of deposition operating conditions. In this review, Ni, NiCo alloy and NiCo–ceramic composite coatings prepared by electrodeposition are reviewed by critically evaluating previous researches. The use of the coatings and their potential for future research and development are discussed.
Curcumin/Usnic Acid-Loaded Electrospun Nanofibers Based on Hyaluronic Acid
Authors: Snetkov Petr , Morozkina Svetlana , Olekhnovich Roman , Vu Thi Hong Nhung , Tyanutova Maria , Uspenskaya Mayya
Published: August 2020

Hyaluronic acid, curcumin, and usnic acid are separately utilized as effective biological agents in medicine, and materials based on its blend are considered to have wider therapeutic effects than individual ones. In this study, for the first time, native hyaluronic acid-based fibers containing curcumin and usnic acid with an average diameter of 298 nm were successfully prepared by the electrospinning technique and characterized. Additionally, unstable and hydrophobic curcumin and usnic acid were loaded into the hydrophilic hyaluronic acid matrix without utilizing the activating (catalyzing) agents, resulting in the formation of an electrospinnable solution. Only the binary mixture deionized water—dimethyl sulfoxide (50:50)—was used as solvent. The presence of small amounts of dimethyl sulfoxide in the fibrous materials was expected to provide the materials with local anesthetic and antiseptic activity. The effect of electric voltage on the electrospinning process, diameter, and morphology of hyaluronic acid/curcumin/usnic acid fibers was investigated in detail. The impact of curcumin and usnic acid on the stability of fiber formation was observed. The investigation of fibers based on pure hyaluronic acid without additional polymers and with active pharmaceutical ingredients will lay the groundwork for the development of highly effective wound dressings and new drug delivery scaffolds.
Correlation among the Power Dissipation Efficiency, Flow Stress Course, and Activation Energy Evolution in Cr-Mo Low-Alloyed Steel
Authors: Opěla Petr , Schindler Ivo , Kawulok Petr , Kawulok Rostislav , Rusz Stanislav , Navrátil Horymír , Jurča Radek
Published: August 2020

In the presented research, conventional hot processing maps superimposed over the flow stress maps or activation energy maps are utilized to study a correlation among the efficiency of power dissipation, flow stress, and activation energy evolution in the case of Cr-Mo low-alloyed steel. All maps have been assembled on the basis of two flow curve datasets. The experimental one is the result of series of uniaxial hot compression tests. The predicted one has been calculated on the basis of the subsequent approximation procedure via a well-adapted artificial neural network. It was found that both flow stress and activation energy evolution are capable of expressing changes in the studied steel caused by the hot compression deformation. A direct association with the course of power dissipation efficiency is then evident in the case of both. The connection of the presence of instability districts to the activation energy evolution, flow stress course, and power dissipation efficiency was discussed further. Based on the obtained findings it can be stated that the activation energy processing maps represent another tool for the finding of appropriate forming conditions and can be utilized as a support feature for the conventionally-used processing maps to extend their informative ability.
Ultra-Sensitive Affordable Cementitious Composite with High Mechanical and Microstructural Performances by Hybrid CNT/GNP
Authors: Abedi Mohammadmahdi , Fangueiro Raul , Gomes Correia António
Published: August 2020

In this paper a hybrid combination of carbon nanotubes (CNTs) and graphene nanoplatelets (GNPs) was used for developing cementitious self-sensing composite with high mechanical, microstructural and durability performances. The mixture of these two nanoparticles with different 1D and 2D geometrical shapes can reduce the percolation threshold to a certain amount which can avoid agglomeration formation and also reinforce the microstructure due to percolation and electron quantum tunneling amplification. In this route, different concentrations of CNT + GNP were dispersed by Pluronic F-127 and tributyl phosphate (TBP) with 3 h sonication at 40 °C and incorporated into the cementitious mortar. Mechanical, microstructural, and durability of the reinforced mortar were investigated by various tests in different hydration periods (7, 28, and 90 days). Additionally, the piezoresistivity behavior of specimens was also evaluated by the four-probe method under flexural and compression cyclic loading. Results demonstrated that hybrid CNT + GNP can significantly improve mechanical and microstructural properties of cementitious composite by filler function, bridging cracks, and increasing hydration rate mechanisms. CNT + GNP intruded specimens also showed higher resistance against climatic cycle tests. Generally, the trend of all results demonstrates an optimal concentration of CNT (0.25%) + GNP (0.25%). Furthermore, increasing CNT + GNP concentration leads to sharp changes in electrical resistivity of reinforced specimens under small variation of strain achieving high gauge factor in both flexural and compression loading modes.
Practice of Simulation and Life Cycle Assessment in Tribology—A Review
Authors: Kurdi Abdulaziz , Alhazmi Nahla , Alhazmi Hatem , Tabbakh Thamer
Published: August 2020

To simulate today’s complex tribo-contact scenarios, a methodological breakdown of a complex design problem into simpler sub-problems is essential to achieve acceptable simulation outcomes. This also helps to manage iterative, hierarchical systems within given computational power. In this paper, the authors reviewed recent trends of simulation practices in tribology to model tribo-contact scenario and life cycle assessment (LCA) with the help of simulation. With the advancement of modern computers and computing power, increasing effort has been given towards simulation, which not only saves time and resources but also provides meaningful results. Having said that, like every other technique, simulation has some inherent limitations which need to be considered during practice. Keeping this in mind, the pros and cons of both physical experiments and simulation approaches are reviewed together with their interdependency and how one approach can benefit the other. Various simulation techniques are outlined with a focus on machine learning which will dominate simulation approaches in the future. In addition, simulation of tribo-contacts across different length scales and lubrication conditions is discussed in detail. An extension of the simulation approach, together with experimental data, can lead towards LCA of components which will provide us with a better understanding of the efficient usage of limited resources and conservation of both energy and resources.
Laser Powder Bed Fusion of Metal Coated Copper Powders
Authors: Lindström Viktor , Liashenko Oleksii , Zweiacker Kai , Derevianko Serhii , Morozovych Vladyslav , Lyashenko Yurij , Leinenbach Christian
Published: August 2020

Laser powder bed fusion (L-PBF) of copper alloys with high copper content is difficult due to the high infrared reflectivity and thermal conductivity of these alloys. In this study a simple and scalable method for coating copper powder with tin and nickel is presented, and suggested as an alloying strategy for such alloys. The coated powders were processed in a commercial L-PBF-machine at various scanning speeds. The samples made from coated powders show a lower amount of porosity compared to samples made from in-situ alloyed powders of similar composition.
Amino Functionalized Micro-Mesoporous Hybrid Particles for the Sustained Release of the Antiretroviral Drug Tenofovir
Authors: Martin-Illana Araceli , Cazorla-Luna Raul , Notario-Pérez Fernando , Ruiz-Caro Roberto , Bedoya Luis Miguel , Veiga-Ochoa Maria Dolores , Rubio Juan , Tamayo Aitana
Published: August 2020

The sustained release of an antiretroviral agent to women mucosa has been proved as an excellent strategy to reduce the sexual transmission of HIV. Hybrid micro-mesoporous particles have been synthesized and functionalized with a silane coupling agent followed by loading the antiretroviral tenofovir. It has been observed that the disposition of the silane molecule on the surface of the particles determines the interaction mechanism with the antiretroviral molecule loaded independently on the surface area of the particles. In this sense, available and free amino groups are required to achieve a smart pH-responsive material, a condition that is only achieved in those materials containing a silane chemisorbed monolayer. Moreover, the modulation of the release kinetics attributed to the presence of the silane monolayer covering the mesopores has been confirmed by fitting the releasing curves to the first order and Weibull models. The developed micro-mesoporous particles have been demonstrated to be excellent smart-release vehicles for antiviral agents and can be safely used in polymer mucoadhesive vaginal gels.
Assessment of Rough Set Theory in Relation to Risks Regarding Hydraulic Engineering Investment Decisions
Authors: Qu Jihong , Bai Xiao , Gu Jiajun , Taghizadeh-Hesary Farhad , Lin Ji
Published: August 2020

Rough set theory is a mathematics tool specifying imperfection and uncertainty. Based on the knowledge theory of the rough set, the numerical values of some features or attributes are not required. Through data reduction, this article analyzes the investment decision of hydraulic engineering and obtains the following by reduction: i. when the construction expense of the hydraulic engineering is low, but the financial income is high, the investment in the construction project can be selected; ii. when the expense of the hydraulic construction project is low and the external influence is common or the financial expense is common, if the external influence is low or the financial income is high, the investment can be delayed; iii. when the construction strategic benefit of the hydraulic engineering is low, the decision rule of no investment can be selected. The novel findings discovered by this article have provided scientific information regarding investment decisions of hydraulic engineering.
Response to Three Weeks of Sprint Interval Training Cannot Be Explained by the Exertional Level
Authors: Krusnauskas Raulas , Eimantas Nerijus , Baranauskiene Neringa , Venckunas Tomas , Snieckus Audrius , Brazaitis Marius , Westerblad Hakan , Kamandulis Sigitas
Published: August 2020

Background and Objectives: The all-out mode of sprint interval training (SIT) has been shown to be an efficient method for improving sports performance, exercise capacity, and aerobic fitness. Although the benefits of SIT are well described, the mechanisms underlying the different degrees of response remain largely unexplored. We aimed to assess the effects of exertion on the responsiveness to SIT. Materials and Methods: The participants were 28 young untrained men (mean ± SD age 25.7 ± 6.03 years) who exhibited either a large or small increase in Wingate test average power in response to nine SIT sessions performed over three weeks. Each training session comprised four–six bouts of 30 s all-out cycling interspaced with 4 min of rest. Individual responses were assessed using heart rate (HR) during exercise for all nine sessions, as well as blood lactate concentration up to 1 h, and the decrement in maximal voluntary knee extension torque (MVC) up to 24 h after the first and last training sessions. Peak oxygen uptake (VO2peak) and maximum HR were measured before and after training during an incremental cycling test to exhaustion. Results: Although all participants showed benefits of SIT such as increased VO2peak, the increase in anaerobic cycling power varied between participants. We identified 17 high responders and nine low responders, whose average power outputs were 0.80 ± 0.22 and 0.22 ± 0.19 W/kg, respectively. The HR achieved during any of the training sessions did not differ between high and low responders. The lactate kinetics did not differ between groups before and after the intervention. Training resulted in a more rapid recovery of MVC without any discernible differences between the high and low responders. Conclusion: The differences in the responses to SIT are not dependent on the exertion level during training.
Preparation of V–4Cr–4Ti Alloys from Mixed Oxides via Electro-Deoxidation Process in Molten Salt
Authors: Cao Xiaozhou , Li Qiuyue , Shi Yuanyuan , Wu Dong , Xue Xiangxin
Published: August 2020

V–4Cr–4Ti alloys exhibit important advantages as a candidate structural materials for fusion reactor first-walls and blanket applications. V–4Cr–4Ti alloys were prepared by direct electrochemical reduction of the solid mixture of V2O3, Cr2O3 and TiO2 in the molten CaCl2–NaCl eutectic at 1073 K. The influence of cell voltage, sintering temperature and electrolysis time on the electrolysis process are reported. The microstructure and phase compositions of the products were analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) during the electrolysis process. The results showed that V–4Cr–4Ti alloys can be obtained at the voltage of 3.1 V and the time of 0.5 h. Cr2O3 was first reduced to Cr metal, while V2O3 and TiO2 was reduced to low-valence oxide of vanadium and titanium. The reduction rate increases with increasing cell voltage, with much perovskite oxide formed during the electrolysis process.
Microstructure and Wear Resistance of TiB2/7075 Composites Produced via Rheocasting
Authors: Gao Qian , Yang Bin , Gan Guisheng , Zhong Yujie , Sun Liang , Zhai Wenyan , Qiang Wei , Wang Shiqing , Lu Yongxin
Published: August 2020

In this study, TiB2/7075 aluminum matrix composites were prepared via in situ synthesis. It was found that TiB2 particles are mainly quadrate. Large TiB2 particles (1–2 μm) agglomerate at grain boundaries, but most of the particles are on the submicron scale. Adding 4.5 wt.% TiB2 particles effectively optimizes α-Al grains in the 7075 aluminum alloy. By combining in situ reinforcing particles with the self-stirring effect of a serpentuator, rheocasting of the 7075 aluminum alloy was achieved in a simple and economical way. The average grain size of the specimen after rheocasting and heat treatment was smaller than 33 μm, and the shape factors were greater than 0.85. The wear resistance of the 4.5 wt.% TiB2/7075 aluminum matrix composite that was prepared via rheocasting and gravity casting was tested with loads of 30, 60, 90, and 120 N at a friction speed of 0.15 m/s for a duration of 30 min. Because of the optimized microstructure and increased hardness, the wear resistance of the 4.5 wt.% TiB2/7075 aluminum matrix composite was significantly better than that of the 7075 aluminum alloy, and the wear resistance of the rheocast TiB2/7075 aluminum matrix composite was better than that of the gravity cast one.
Formation of Spessartine and CO2 via Rhodochrosite Decarbonation along a Hot Subduction P-T Path
Authors: Bataleva Yuliya V. , Kruk Aleksei N. , Novoselov Ivan D. , Palyanov Yuri N.
Published: August 2020

Experimental simulation of rhodochrosite-involving decarbonation reactions resulting in the formation of spessartine and CO2-fluid was performed in a wide range of pressures (P) and temperatures (T) corresponding to a hot subduction P-T path. Experiments were carried out using a multi-anvil high-pressure apparatus of a “split-sphere” type (BARS) in an MnCO3–SiO2–Al2O3 system (3.0–7.5 GPa, 850–1250 °C and 40–100 h.) with a specially designed high-pressure hematite buffered cell. It was experimentally demonstrated that decarbonation in the MnCO3–SiO2–Al2O3 system occurred at 870 ± 20 °C (3.0 GPa), 1070 ± 20 °C (6.3 GPa), and 1170 ± 20 °C (7.5 GPa). Main Raman spectroscopic modes of the synthesized spessartine were 349–350 (R), 552(υ2), and 906–907 (υ1) cm−1. As evidenced by mass spectrometry (IRMS) analysis, the fluid composition corresponded to pure CO2. It has been experimentally shown that rhodochrosite consumption to form spessartine + CO2 can occur at conditions close to those of a hot subduction P-T path but are 300–350 °C lower than pyrope + CO2 formation parameters at constant pressures. We suppose that the presence of rhodocrosite in the subducting slab, even as solid solution with Mg,Ca-carbonates, would result in a decrease of the decarbonation temperatures. Rhodochrosite decarbonation is an important reaction to explain the relationship between Mn-rich garnets and diamonds with subduction/crustal isotopic signature.
Comparison of Enhanced Photocatalytic Degradation Efficiency and Toxicity Evaluations of CeO2 Nanoparticles Synthesized Through Double-Modulation
Authors: Choi Jang Hyun , Hong Jung-A , Son Ye Rim , Wang Jian , Kim Hyun Sung , Lee Hansol , Lee Hangil
Published: August 2020

We demonstrated that Fe/Cr doped and pH-modified CeO2 nanoparticles (NPs) exhibit enhanced photocatalytic performance as compared to bare CeO2 NPs, using photocatalytic degradation. To assess the toxicity level of these double-modified CeO2 NPs on the human skin, they were introduced into HaCaT cells. The results of our conventional cellular toxicity assays (neutral red uptake and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide for assays) indicated that Cr@CeOx NPs prompt severe negative effects on the viability of human cells. Moreover, the results obtained by scanning transmission X-ray microscopy and bio-transmission electron microscope analysis showed that most of the NPs were localized outside the nucleus of the cells. Thus, serious genetic toxicity was unlikely. Overall, this study highlights the need to prevent the development of Cr@CeOx NP toxicity. Moreover, further research should aim to improve the photocatalytic properties and activity of these NPs while accounting for their stability issues.
Antibacterial Activity of Manganese Dioxide Nanosheets by ROS-Mediated Pathways and Destroying Membrane Integrity
Authors: Du Ting , Chen Siya , Zhang Jinyu , Li Tingting , Li Ping , Liu Jifeng , Du Xinjun , Wang Shuo
Published: August 2020

Manganese dioxide (MnO2) nanosheets have shown exciting potential in nanomedicine because of their ultrathin thickness, large surface area, high near-infrared (NIR) absorbance and good biocompatibility. However, the effect of MnO2 nanosheets on bacteria is still unclear. In this study, MnO2 nanosheets were shown for the first time to possess highly efficient antibacterial activity by using Salmonella as a model pathogen. The growth curve and surface plate assay uncovered that 125 μg/mL MnO2 nanosheets could kill 99.2% of Salmonella, which was further verified by fluorescence-based live/dead staining measurement. Mechanism analysis indicated that MnO2 nanosheet treatment could dramatically induce reactive oxygen species production, increase ATPase activity and cause the leakage of electrolytes and protein contents, leading to bacterial death. These results uncover the previously undefined role of MnO2 nanosheets and provide novel strategies for developing antimicrobial agents.
Separation of Radionuclides from Spent Decontamination Fluids via Adsorption onto Titanium Dioxide Nanotubes after Photocatalytic Degradation
Authors: Lyczko Monika , Wiaderek Barbara , Bilewicz Aleksander
Published: August 2020

A one-step process combining the photocatalytic degradation of radionuclide complexes and the adsorption of liberated radionuclides on titanium dioxide nanotubes was developed and tested for the purification of aqueous waste produced from chemical decontamination of nuclear power plant circuit components. Among the tested forms of TiO2, only nanotubes exhibit both high photocatalytic activity and sorption ability, which support their application in a one-step purification process. The obtained results indicate that the photocatalytic degradation of complexes followed by the sorption of the radionuclides onto TiO2 nanotubes offers a promising route for treating spent decontamination fluids.
Nanostructured Color Filters: A Review of Recent Developments
Authors: Shaukat Ayesha , Noble Frazer , Arif Khalid Mahmood
Published: August 2020

Color plays an important role in human life: without it life would be dull and monochromatic. Printing color with distinct characteristics, like hue, brightness and saturation, and high resolution, are the main characteristic of image sensing devices. A flexible design of color filter is also desired for angle insensitivity and independence of direction of polarization of incident light. Furthermore, it is important that the designed filter be compatible with the image sensing devices in terms of technology and size. Therefore, color filter requires special care in its design, operation and integration. In this paper, we present a comprehensive review of nanostructured color filter designs described to date and evaluate them in terms of their performance.
Sociodemographic Differences in the Dietary Quality of Food-at-Home Acquisitions and Purchases among Participants in the U.S. Nationally Representative Food Acquisition and Purchase Survey (FoodAPS)
Authors: Vadiveloo Maya K. , Parker Haley W. , Juul Filippa , Parekh Niyati
Published: August 2020

Insufficient research has explored whether sociodemographic differences in self-reported, individual-level diet quality are similarly reflected by grocery purchase quality. This cross-sectional analysis of n = 3961 U.S. households from the nationally representative Food Acquisition and Purchase Survey (FoodAPS) compared Healthy Eating Index (HEI)-2015 scores from 1 week of food-at-home acquisitions across self-reported demographic factors (race/ethnicity, Supplemental Nutrition Assistance Program (SNAP) participation, food security, and household-level obesity status). Multivariable-adjusted, survey-weighted regression models compared household HEI-2015 scores across sociodemographic groups. Respondents were primarily White and female, with a mean age of 50.6 years, 14.0% were food insecure, and 12.7% were SNAP-participating. Mean HEI-2015 scores were 54.7; scores differed across all sociodemographic exposures (p < 0.05). Interactions (p < 0.1) were detected between SNAP participation and (1) food insecurity and (2) household-level obesity, and race/ethnicity and (1) household-level obesity. HEI-2015 scores were higher among food secure, non-SNAP households than among food insecure, SNAP-participating households (53.9 ± 0.5 vs. 50.3 ± 0.7, p = 0.007); non-SNAP households without obesity had significantly higher HEI-2015 scores than other households. Household-level obesity was associated with lower HEI-2015 scores in White (50.8 ± 0.5 vs. 52.5 ± 0.7, p = 0.046) and Black (48.8 ± 1.5 vs. 53.1 ± 1.4, p = 0.018) but not Hispanic households (54.4 ± 1.0 vs. 52.2 ± 1.2, p = 0.21). Sociodemographic disparities in household HEI-2015 scores were consistent with previous research on individual-level diet quality.
An Open Pilot Study of the Effect and Tolerability of Add-On Multivitamin Therapy in Patients with Intractable Focal Epilepsy
Authors: Chang Hui Hua , Sung Pi-Shan , Liao Wei Chen , Chang Alice Y. W. , Hsiao Ya-Hsin , Fu Tzu-Fun , Huang Chin-Ying , Huang Chin-Wei
Published: August 2020

Observational studies have investigated the potential modulatory effect of neuronal excitability by vitamins in epilepsy. We aimed to investigate whether the addition of multivitamin therapy (B6/B9, D, E and Q) to regular antiepileptic drug therapy could ameliorate seizures in patients with refractory focal epilepsy. We conducted a prospective cohort open study to investigate the effect and tolerability of add-on multivitamin therapy (daily dose: B6 100 mg, B9 5 mg, D 1000 IU, E 400 IU and coenzyme Q10 100 mg) in patients with intractable focal epilepsy. All patients had effect and safety assessments at baseline and after one, three and six months of the supplementation. Thirty patients (11 men and 19 women) with a mean age of 42.37 ± 9.40 years were recruited and four patients discontinued. The seizure frequency significantly decreased after the six-month supplementation (9.04 ± 18.16/month and 2.06 ± 3.89/month, p = 0.045). At the final visit, 62.5% of the patients showed a ≥50% reduction in seizure frequency, and 12.5% were seizure-free. As to safety and tolerability, most patients did not experience significant adverse events, although three patients reported seizure worsening. In conclusion, this pilot study demonstrated the therapeutic potential and essentially good tolerability of add-on multivitamin therapy in patients with refractory focal epilepsy.
Gut Microbiota Associations with Metabolic Health and Obesity Status in Older Adults
Authors: Zhong Xiaozhong , Harrington Janas M. , Millar Seán R. , Perry Ivan J. , O’Toole Paul W. , Phillips Catherine M.
Published: August 2020

Emerging evidence links the gut microbiota with several chronic diseases. However, the relationships between metabolic syndrome (MetS), obesity and the gut microbiome are inconsistent. This study aimed to investigate associations between gut microbiota composition and diversity and metabolic health status in older adults (n = 382; median age = 69.91 [± 5 years], male = 50.79%) with and without obesity. Gut microbiome composition was determined by sequencing 16S rRNA gene amplicons. Results showed that alpha diversity and richness, as indicated by the Chao1 index (p = 0.038), phylogenetic diversity (p = 0.003) and observed species (p = 0.038) were higher among the metabolically healthy non-obese (MHNO) individuals compared to their metabolically unhealthy non-obese (MUNO) counterparts. Beta diversity analysis revealed distinct differences between the MHNO and MUNO individuals on the phylogenetic distance scale (R2 = 0.007, p = 0.004). The main genera contributing to the gut composition among the non-obese individuals were Prevotella, unclassified Lachnospiraceae, and unclassified Ruminococcaceae. Prevotella, Blautia, Bacteroides, and unclassified Ruminococcaceae mainly contributed to the variation among the obese individuals. Co-occurrence network analysis displayed different modules pattern among different metabolic groups and revealed groups of microbes significantly correlated with individual metabolic health markers. These findings confirm relationships between metabolic health status and gut microbiota composition particularly, among non-obese older adults.
Ultra-Processed Food Consumption Is Associated with Abdominal Obesity: A Prospective Cohort Study in Older Adults
Authors: Sandoval-Insausti Helena , Jiménez-Onsurbe Manuel , Donat-Vargas Carolina , Rey-García Jimena , Banegas José R. , Rodríguez-Artalejo Fernando , Guallar-Castillón Pilar
Published: August 2020

Introduction and objectives. Ultra-processed food (UPF) consumption has been associated with increased incidence of cardiovascular disease and its risk factors. The aim of this study was to assess, for the first time in the literature, the prospective association between UPF consumption and the incidence of abdominal obesity (AO) in older adults. Methods. The study sample consists of 652 participants in the Seniors Study on Nutrition and Cardiovascular Risk in Spain: Seniors-ENRICA-1 study, (mean age 67, 44% women). At baseline, standardized anthropometric measurements were collected (including abdominal circumference). After a median follow-up of six years, the abdominal circumference was measured again, and the incidence of abdominal obesity (AO) was calculated, defined as an abdominal perimeter ≥102 cm in men and ≥88 cm in women. At baseline, dietary information was collected using a computerized and validated dietary history. Information was obtained on the usual diet in the previous year. A total number of 880 foods were classified according to their degree of processing following the NOVA classification. Foods or drinks formulated mostly or entirely from substances derived from foods, with little or no presence of the unaltered original food were classified as UPF. For each participant, the percentage of energy from UPF was derived and sex-specific tertiles were calculated. Logistic regression models were built and adjusted for sociodemographic, lifestyle, morbidity, and drug treatment variables. Results. Among those participants without AO at baseline, 177 developed AO during follow-up. The average consumption of UPF was 17% of total energy (7% in the first tertile; 29% in the third tertile). The odds ratio (95% confidence interval) for incident AO risk when compared to the lowest tertile was: 1.55 (0.99–2.44) for the second tertile of UPF consumption and 1.62 (1.04–2.54) for the third tertile; p for linear trend: 0.037. Results remained statistically significant after adjusting for potential dietary confounding factors such as fiber consumption, the intake of very long chain omega-3 fatty acids and adherence to the Mediterranean diet. Conclusions. A higher UPF consumption is positively associated with incident AO in older adults in Spain. These findings extend the current evidence of the detrimental effect of UPF consumption on cardiometabolic health.
Antimicrobial Resistance and Biofilm Formation Capacity of Salmonella enterica Serovar Enteritidis Strains Isolated from Poultry and Humans in Poland
Authors: Ćwiek Katarzyna , Korzekwa Kamila , Tabiś Aleksandra , Bania Jacek , Bugla-Płoskońska Gabriela , Wieliczko Alina
Published: August 2020

Salmonella enterica ser. Enteritidis (S. enterica ser. Enteritidis) is the most frequently detected serovar in human salmonellosis, and its ability to produce a biofilm and the risk of transmission from animals and food of animal origin to humans are significant. The main aim of the present work was to compare S. enterica ser. Enteritidis strains isolated from poultry and human feces in terms of resistance profiles, prevalence of selected resistance genes, and their potential for biofilm formation, by assessing their biofilm growth intensity, the prevalence and expression of selected genes associated with this phenomenon, and the correlation between increased antimicrobial resistance and biofilm formation ability of the two tested groups of S. enterica ser. Enteritidis. This study showed a difference in antimicrobial resistance (minimal inhibitory concentration value) between S. enterica ser. Enteritidis groups; however, the majority of multidrug-resistant (MDR) strains were isolated from poultry (environmental samples from chicken broilers, turkey broilers, and laying hens). Differences in the prevalence of resistance genes were observed; the most common gene among poultry strains was floR, and that among strains from humans was blaTEM. S. enterica ser. Enteritidis strains isolated from poultry under the tested incubation conditions exhibited better biofilm growth than strains isolated from humans. A higher level of gene expression associated with the production of cellulose was only detected in the S48 strain isolated from poultry. On the other hand, increased expression of genes associated with quorum sensing was observed in two strains isolated from poultry farms and one strain isolated from human feces.
Comparative In Vitro Toxicology of Novel Cytoprotective Short-Chain Naphthoquinones
Authors: Feng Zikai , Sedeeq Mohammed , Daniel Abraham , Corban Monika , Woolley Krystel L. , Condie Ryan , Azimi Iman , Smith Jason A. , Gueven Nuri
Published: August 2020

Short-chain quinones (SCQs) have been identified as potential drug candidates against mitochondrial dysfunction, which largely depends on the reversible redox characteristics of the active quinone core. We recently identified 11 naphthoquinone derivatives, 1–11, from a library of SCQs that demonstrated enhanced cytoprotection and improved metabolic stability compared to the clinically used benzoquinone idebenone. Since the toxicity properties of our promising SCQs were unknown, this study developed multiplex methods and generated detailed toxicity profiles from 11 endpoint measurements using the human hepatocarcinoma cell line HepG2. Overall, the toxicity profiles were largely comparable across different assays, with simple standard assays showing increased sensitivity compared to commercial toxicity assays. Within the 11 naphthoquinones tested, the L-phenylalanine derivative 4 consistently demonstrated the lowest toxicity across all assays. The results of this study not only provide useful information about the toxicity features of SCQs but will also enable the progression of the most promising drug candidates towards their clinical use.
GNSS-Based Machine Learning Storm Nowcasting
Authors: Łoś Marcelina , Smolak Kamil , Guerova Guergana , Rohm Witold
Published: August 2020

Nowcasting of severe weather events and summer storms, in particular, are intensively studied as they have great potential for large economic and societal losses. Use of Global Navigation Satellite Systems (GNSS) observations for weather nowcasting has been investigated in various regions. However, combining the vertically integrated water vapour (IWV) with vertical profiles of wet refractivity derived from GNSS tomography has not been exploited for short-range forecasts of storms. In this study, we introduce a methodology to use the synergy of IWV and tomography-based vertical profiles to predict 0–2 h of storms using a machine learning approach for Poland. Moreover, we present an analysis of the importance of features that take part in the prediction process. The accuracy of the model reached over 87%, and the precision of prediction was about 30%. The results show that wet refractivity below 6 km and IWV on the west of the storm are among the significant parameters with potential for predicting storm location. The analysis of IWV demonstrated a correlation between IWV changes and storm occurrence.
Use of Moon Observations for Characterization of Sentinel-3B Ocean and Land Color Instrument
Authors: Neneman Maciej , Wagner Sébastien , Bourg Ludovic , Blanot Laurent , Bouvet Marc , Adriaensen Stefan , Nieke Jens
Published: August 2020

During the commissioning of the Sentinel-3B satellite, a single lunar observation was performed to assess the possible use of the moon for characterization and validation of onboard instruments. The observation was carried out in stable orientation after a roll maneuver, allowing the moon to be imaged by the Earth view of instruments. Data acquired by the Ocean Land Color Instrument (OLCI) allowed inflight verification of stray-light correction (SLC) performed by the Mission Performance Centre (MPC), and assessment of radiometric behavior of instrument in comparison with lunar irradiance models performed in cooperation between European Space Research and Technology Centre (ESTEC) and European Organisation for the Exploitation of Meteorological Satellites (EUMETSAT). This paper describes the results of those activities along with the proposed update of stray-light correction developed with the use of lunar data.
Small-Satellite Synthetic Aperture Radar for Continuous Global Biospheric Monitoring: A Review
Authors: Paek Sung Wook , Balasubramanian Sivagaminathan , Kim Sangtae , de Weck Olivier
Published: August 2020

Space-based radar sensors have transformed Earth observation since their first use by Seasat in 1978. Radar instruments are less affected by daylight or weather conditions than optical counterparts, suitable for continually monitoring the global biosphere. The current trends in synthetic aperture radar (SAR) platform design are distinct from traditional approaches in that miniaturized satellites carrying SAR are launched in multiples to form a SAR constellation. A systems engineering perspective is presented in this paper to track the transitioning of space-based SAR platforms from large satellites to small satellites. Technological advances therein are analyzed in terms of subsystem components, standalone satellites, and satellite constellations. The availability of commercial satellite constellations, ground stations, and launch services together enable real-time SAR observations with unprecedented details, which will help reveal the global biomass and their changes owing to anthropogenic drivers. The possible roles of small satellites in global biospheric monitoring and the subsequent research areas are also discussed.