bluuebunny/crossref_metadata_2025 huggingface dataset:
Tibolone Pre-Treatment Ameliorates the Dysregulation of Protein Translation and Transport Generated by Palmitic Acid-Induced Lipotoxicity in Human Astrocytes: A Label-Free MS-Based Proteomics and Network Analysis
Authors: Vesga-Jiménez Diego Julián , Martín-Jiménez Cynthia A. , Grismaldo Rodríguez Adriana , Aristizábal-Pachón Andrés Felipe , Pinzón Andrés , Barreto George E. , Ramírez David , González Janneth
Published: June 2022

Excessive accumulation and release of fatty acids (FAs) in adipose and non-adipose tissue are characteristic of obesity and are associated with the leading causes of death worldwide. Chronic exposure to high concentrations of FAs such as palmitic acid (pal) is a risk factor for developing different neurodegenerative diseases (NDs) through several mechanisms. In the brain, astrocytic dysregulation plays an essential role in detrimental processes like metabolic inflammatory state, oxidative stress, endoplasmic reticulum stress, and autophagy impairment. Evidence shows that tibolone, a synthetic steroid, induces neuroprotective effects, but its molecular mechanisms upon exposure to pal remain largely unknown. Due to the capacity of identifying changes in the whole data-set of proteins and their interaction allowing a deeper understanding, we used a proteomic approach on normal human astrocytes under supraphysiological levels of pal as a model to induce cytotoxicity, finding changes of expression in proteins related to translation, transport, autophagy, and apoptosis. Additionally, tibolone pre-treatment showed protective effects by restoring those same pal-altered processes and increasing the expression of proteins from cell survival processes. Interestingly, ARF3 and IPO7 were identified as relevant proteins, presenting a high weight in the protein-protein interaction network and significant differences in expression levels. These proteins are related to transport and translation processes, and their expression was restored by tibolone. This work suggests that the damage caused by pal in astrocytes simultaneously involves different mechanisms that the tibolone can partially revert, making tibolone interesting for further research to understand how to modulate these damages.
CpG Methylation Altered the Stability and Structure of the i-Motifs Located in the CpG Islands
Authors: Oshikawa Daiki , Inaba Shintaro , Kitagawa Yudai , Tsukakoshi Kaori , Ikebukuro Kazunori
Published: June 2022

Cytosine methylation within the 5′-C-phosphate-G-3′ sequence of nucleotides (called CpG methylation) is a well-known epigenetic modification of genomic DNA that plays an important role in gene expression and development. CpG methylation is likely to be altered in the CpG islands. CpG islands are rich in cytosine, forming a structure called the i-motif via cytosine-cytosine hydrogen bonding. However, little is known about the effect of CpG methylation on the i-motif. In this study, The CpG methylation-induced structural changes on the i-motif was examined by thermal stability, circular dichroism (CD) spectroscopy, and native-polyacrylamide gel electrophoresis (Native-PAGE) evaluation of five i-motif-forming DNAs from four cancer-related genes (VEGF, C-KIT, BCL2, and HRAS). This research shows that CpG methylation increased the transitional pH of several i-motif-forming DNAs and their thermal stability. When examining the effect of CpG methylation on the i-motif in the presence of opposite G4-forming DNAs, CpG methylation influenced the proportion of G4 and i-motif formation. This study showed that CpG methylation altered the stability and structure of the i-motif in CpG islands.
Editorial of the Special Issue: cGMP-Signaling in Cells and Tissues: Molecular, Functional and Pharmacological Aspects
Authors: Schlossmann Jens
Published: June 2022

Several important and novel aspects regarding signaling by cGMP were reported in the various publications of this Special Issue [...]
Particulate Matter Exacerbates the Death of Dopaminergic Neurons in Parkinson’s Disease through an Inflammatory Response
Authors: Choi Dabin , Lee Gaheon , Kim Kyung Hwa , Bae Hyunsu
Published: June 2022

Particulate matter (PM), a component of air pollution, has been epidemiologically associated with a variety of diseases. Recent reports reveal that PM has detrimental effects on the brain. In this study, we aimed to investigate the biological effects of ambient particles on the neurodegenerative disease Parkinson’s disease (PD). We exposed mice to coarse particles (PM10: 2.5–10 μm) for short (5 days) and long (8 weeks) durations via intratracheal instillation. Long-term PM10 exposure exacerbated motor impairment and dopaminergic neuron death in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse models. Short-term PM10 exposure resulted in both pulmonary and systemic inflammatory responses in mice. We further investigated the mechanism underlying PM10-induced neurotoxicity in cocultures of lung LA-4 epithelial cells and RAW264.7 macrophages. PM10 treatment elicited a dramatic increase in proinflammatory mediators in LA-4/RAW264.7 coculture. Treating BV2 microglial cells with PM10-treated conditioned medium induced microglial activation. Furthermore, 1-methyl-4-phenylpyridinium (MPP+) treatment caused notable cell death in N2A neurons cocultured with activated BV2 cells in PM10-conditioned medium. Altogether, our results demonstrated that PM10 plays a role in the neurodegeneration associated with PD. Thus, the impact of PM10 on neurodegeneration could be related to detrimental air pollution-induced systemic effects on the brain.
N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper
Authors: Hussain Ansar , Kaisheng Liu , Noman Ali , Ashraf Muhammad Furqan , Albaqami Mohammed , Khan Muhammad Ifnan , Liu Zhiqin , He Shuilin
Published: June 2022

Proteins with conserved SET domain play a critical role in plant immunity. However, the means of organization and functions of these proteins are unclear, particularly in non-model plants such as pepper (Capsicum annum L.). Herein, we functionally characterized CaASHH3, a member of class II (the ASH1 homologs H3K36) proteins in pepper immunity against Ralstonia solanacearum and Pseudomonas syringae pv tomato DC3000 (Pst DC3000). The CaASHH3 was localized in the nucleus, and its transcript levels were significantly enhanced by R. solanacearum inoculation (RSI) and exogenous application of salicylic acid (SA), methyl jasmonate (MeJA), ethephon (ETH), and abscisic acid (ABA). Knockdown of CaASHH3 by virus-induced gene silencing (VIGS) compromised peppers’ resistance to RSI. Furthermore, silencing of CaASHH3 impaired hypersensitive-response (HR)-like cell death response due to RSI and downregulated defense-associated marker genes, including CaPR1, CaNPR1, and CaABR1. The CaASHH3 protein was revealed to affect the promoters of CaNPR1, CaPR1, and CaHSP24. Transiently over-expression of CaASHH3 in pepper leaves elicited HR-like cell death and upregulated immunity-related marker genes. To further study the role of CaASHH3 in plant defense in vivo, CaASHH3 transgenic plants were generated in Arabidopsis. Overexpression of CaASHH3 in transgenic Arabidopsis thaliana enhanced innate immunity against Pst DC3000. Furthermore, CaASHH3 over-expressing transgenic A. thaliana plants exhibited upregulated transcriptional levels of immunity-associated marker genes, such as AtNPR1, AtPR1, and AtPR2. These results collectively confirm the role of CaASHH3 as a positive regulator of plant cell death and pepper immunity against bacterial pathogens, which is regulated by signaling synergistically mediated by SA, JA, ET, and ABA.
The Combined Inactivation of Intestinal and Hepatic ZIP14 Exacerbates Manganese Overload in Mice
Authors: Fung Caitlin K. , Zhao Ningning
Published: June 2022

ZIP14 is a newly identified manganese transporter with high levels of expression in the small intestine and the liver. Loss-of-function mutations in ZIP14 can lead to systemic manganese overload, which primarily affects the central nervous system, causing neurological disorders. To elucidate the roles of intestinal ZIP14 and hepatic ZIP14 in maintaining systemic manganese homeostasis, we generated mice with single-tissue or two-tissue Zip14 knockout, including intestine-specific (Zip14-In-KO), liver-specific (Zip14-L-KO), and double (intestine and liver) Zip14-knockout (Zip14-DKO) mice. Zip14flox/flox mice were used as the control. Tissue manganese contents in these mice were compared using inductively coupled plasma mass spectrometry (ICP-MS) analysis. We discovered that although the deletion of intestinal ZIP14 only moderately increased systemic manganese loading, the deletion of both intestinal and hepatic ZIP14 greatly exacerbated the body’s manganese burden. Our results provide new knowledge to further the understanding of manganese metabolism, and offer important insights into the mechanisms underlying systemic manganese overload caused by the loss of ZIP14.
What Genetics Can Do for Oncological Imaging: A Systematic Review of the Genetic Validation Data Used in Radiomics Studies
Authors: Mirón Mombiela Rebeca , Arildskov Anne Rix , Bruun Frederik Jager , Hasselbalch Lotte Harries , Holst Kristine Bærentz , Rasmussen Sine Hvid , Borrás Consuelo
Published: June 2022

(1) Background: Radiogenomics is motivated by the concept that biomedical images contain information that reflects underlying pathophysiology. This review focused on papers that used genetics to validate their radiomics models and outcomes and assess their contribution to this emerging field. (2) Methods: All original research with the words radiomics and genomics in English and performed in humans up to 31 January 2022, were identified on Medline and Embase. The quality of the studies was assessed with Radiomic Quality Score (RQS) and the Cochrane recommendation for diagnostic accuracy study Quality Assessment 2. (3) Results: 45 studies were included in our systematic review, and more than 50% were published in the last two years. The studies had a mean RQS of 12, and the studied tumors were very diverse. Up to 83% investigated the prognosis as the main outcome, with the rest focusing on response to treatment and risk assessment. Most applied either transcriptomics (54%) and/or genetics (35%) for genetic validation. (4) Conclusions: There is enough evidence to state that new science has emerged, focusing on establishing an association between radiological features and genomic/molecular expression to explain underlying disease mechanisms and enhance prognostic, risk assessment, and treatment response radiomics models in cancer patients.
Tissue-Specific Downregulation of Fatty Acid Synthase Suppresses Intestinal Adenoma Formation via Coordinated Reprograming of Transcriptome and Metabolism in the Mouse Model of Apc-Driven Colorectal Cancer
Authors: Drury James , Young Lyndsay E. A. , Scott Timothy L. , Kelson Courtney O. , He Daheng , Liu Jinpeng , Wu Yuanyan , Wang Chi , Weiss Heidi L. , Fan Teresa , Gentry Matthew S. , Sun Ramon , Zaytseva Yekaterina Y.
Published: June 2022

Altered lipid metabolism is a potential target for therapeutic intervention in cancer. Overexpression of Fatty Acid Synthase (FASN) correlates with poor prognosis in colorectal cancer (CRC). While multiple studies show that upregulation of lipogenesis is critically important for CRC progression, the contribution of FASN to CRC initiation is poorly understood. We utilize a C57BL/6-Apc/Villin-Cre mouse model with knockout of FASN in intestinal epithelial cells to show that the heterozygous deletion of FASN increases mouse survival and decreases the number of intestinal adenomas. Using RNA-Seq and gene set enrichment analysis, we demonstrate that a decrease in FASN expression is associated with inhibition of pathways involved in cellular proliferation, energy production, and CRC progression. Metabolic and reverse phase protein array analyses demonstrate consistent changes in alteration of metabolic pathways involved in both anabolism and energy production. Downregulation of FASN expression reduces the levels of metabolites within glycolysis and tricarboxylic acid cycle with the most significant reduction in the level of citrate, a master metabolite, which enhances ATP production and fuels anabolic pathways. In summary, we demonstrate the critical importance of FASN during CRC initiation. These findings suggest that targeting FASN is a potential therapeutic approach for early stages of CRC or as a preventive strategy for this disease.
Genomic Analysis Made It Possible to Identify Gene-Driver Alterations Covering the Time Window between Diagnosis of Neuroblastoma 4S and the Progression to Stage 4
Authors: Ognibene Marzia , De Marco Patrizia , Parodi Stefano , Meli Mariaclaudia , Di Cataldo Andrea , Zara Federico , Pezzolo Annalisa
Published: June 2022

Neuroblastoma (NB) is a tumor of the developing sympathetic nervous system. Despite recent advances in understanding the complexity of NB, the mechanisms that determine its regression or progression are still largely unknown. Stage 4S NB is characterized by a favorable course of disease and often by spontaneous regression, while progression to true stage 4 is a very rare event. Here, we focused on genomic analysis of an NB case that progressed from stage 4S to stage 4 with a very poor outcome. Array-comparative genomic hybridization (a-CGH) on tumor-tissue DNA, and whole-exome sequencing (WES) on exosomes DNA derived from plasma collected at the onset and at the tumor progression, pointed out relevant genetic changes that can explain this clinical worsening. The combination of a-CGH and WES data allowed for the identification iof somatic copy number aberrations and single-nucleotide variants in genes known to be responsible for aggressive NB. KLRB1, MAPK3 and FANCA genes, which were lost at the time of progression, were studied for their possible role in this event by analyzing in silico the impact of their expression on the outcome of 786 NB patients.
Use of PET Imaging to Assess the Efficacy of Thiethylperazine to Stimulate Cerebral MRP1 Transport Activity in Wild-Type and APP/PS1-21 Mice
Authors: Wölfl-Duchek Michael , Mairinger Severin , Hernández-Lozano Irene , Filip Thomas , Zoufal Viktoria , Löbsch Mathilde , Stanek Johann , Kuntner Claudia , Wanek Thomas , Bauer Martin , Pahnke Jens , Langer Oliver
Published: June 2022

Multidrug resistance-associated protein 1 (MRP1, encoded by the ABCC1 gene) may contribute to the clearance of amyloid-beta (Aβ) peptides from the brain into the blood and stimulation of MRP1 transport activity may be a therapeutic approach to enhance brain Aβ clearance. In this study, we assessed the effect of thiethylperazine, an antiemetic drug which was shown to stimulate MRP1 activity in vitro and to decrease Aβ load in a rapid β-amyloidosis mouse model (APP/PS1-21), on MRP1 transport activity by means of positron emission tomography (PET) imaging with the MRP1 tracer 6-bromo-7-[11C]methylpurine. Groups of wild-type, APP/PS1-21 and Abcc1(−/−) mice underwent PET scans before and after a 5-day oral treatment period with thiethylperazine (15 mg/kg, once daily). The elimination rate constant of radioactivity (kelim) was calculated from time–activity curves in the brain and the lungs as a measure of tissue MRP1 activity. Treatment with thiethylperazine had no significant effect on MRP1 activity in the brain and the lungs of wild-type and APP/PS1-21 mice. This may either be related to a lack of an MRP1-stimulating effect of thiethylperazine in vivo or to other factors, such as substrate-dependent MRP1 stimulation, insufficient target tissue exposure to thiethylperazine or limited sensitivity of the PET tracer to measure MRP1 stimulation.
Hypomethylation of CLDN4 Gene Promoter Is Associated with Malignant Phenotype in Urinary Bladder Cancer
Authors: Maesaka Fumisato , Kuwada Masaomi , Horii Shohei , Kishi Shingo , Fujiwara-Tani Rina , Mori Shiori , Fujii Kiyomu , Mori Takuya , Ohmori Hitoshi , Owari Takuya , Miyake Makito , Nakai Yasushi , Tanaka Nobumichi , Bhawal Ujjal Kumar , Luo Yi , Kondoh Masuo , Fujimoto Kiyohide , Kuniyasu Hiroki
Published: June 2022

The tight junction (TJ) protein claudin-4 (CLDN4) is overexpressed in bladder urothelial carcinoma (BUC) and correlates with cancer progression. However, the mechanism of CLDN4 upregulation and promotion of malignant phenotype is not clear. Here, we analyzed 157 cases of BUC and investigated the hypomethylation of CpG island in the CLDN4 promoter DNA and its correlation with cancer progression. In hypomethylated cases, CLDN4 expression, cell proliferation, stemness, and epithelial-mesenchymal transition were increased. Treatment of three human BUC cell lines with the demethylating agent aza-2′-deoxycytidine (AZA) led to excessive CLDN4 expression, and, specifically, to an increase in CLDN4 monomer that is not integrated into the TJ. The TJ-unintegrated CLDN4 was found to bind integrin β1 and increase stemness, drug resistance, and metastatic ability of the cells as well as show an anti-apoptosis effect likely via FAK phosphorylation, which reduces upon knockdown of CLDN4. Thus, CLDN4 is overexpressed in BUC by an epigenetic mechanism and the high expression enhances the malignant phenotype of BUC via increased levels of TJ-unintegrated CLDN4. CLDN4 promoter DNA methylation is expected to be a novel indicator of BUC malignant phenotype and a new therapeutic target.
GmWRKY81 Encoding a WRKY Transcription Factor Enhances Aluminum Tolerance in Soybean
Authors: Shu Wenjiao , Zhou Qianghua , Xian Peiqi , Cheng Yanbo , Lian Tengxiang , Ma Qibin , Zhou Yonggang , Li Haiyan , Nian Hai , Cai Zhandong
Published: June 2022

Aluminum (Al) toxicity is an essential factor that adversely limits soybean (Glycine max (L.) Merr.) growth in acid soils. WRKY transcription factors play important roles in soybean responses to abiotic stresses. Here, GmWRKY81 was screened from genes that were differentially expressed under Al treatment in Al-tolerant soybean Baxi10 and Al-sensitive soybean Bendi2. We found that GmWRKY81 was significantly induced by 20 μM AlCl3 and upregulated by AlCl3 treatment for 2 h. In different tissues, the expression of GmWRKY81 was differentially induced. In 0–1 cm root tips, the expression of GmWRKY81 was induced to the highest level. The overexpression of GmWRKY81 in soybean resulted in higher relative root elongation, root weight, depth, root length, volume, number of root tips and peroxidase activity but lower root average diameter, malonaldehyde and H2O2 contents, indicating enhanced Al tolerance. Moreover, RNA-seq identified 205 upregulated and 108 downregulated genes in GmWRKY81 transgenic lines. Fifteen of these genes that were differentially expressed in both AlCl3-treated and GmWRKY81-overexpressing soybean had the W-box element, which can bind to the upstream-conserved WRKY domain. Overall, the combined functional analysis indicates that GmWRKY81 may improve soybean Al tolerance by regulating downstream genes participating in Al3+ transport, organic acid secretion and antioxidant reactions.
Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content
Authors: Luo Juan , Abid Muhammad , Tu Jing , Gao Puxing , Wang Zupeng , Huang Hongwen
Published: June 2022

Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays a vital role in photosynthesis. However, the reported knowledge of LHCs in kiwifruit is inadequate and poorly understood. In this study, we identified 42 and 45 LHC genes in Actinidia chinensis (Ac) and A. eriantha (Ae) genomes. Phylogenetic analysis showed that the kiwifruit LHCs of both species were grouped into four subfamilies (Lhc, Lil, PsbS, and FCII). Expression profiles and qRT-PCR results revealed expression levels of LHC genes closely related to the light, temperature fluctuations, color changes during fruit ripening, and kiwifruit responses to Pseudomonas syringae pv. actinidiae (Psa). Subcellular localization analysis showed that AcLhcb1.5/3.1/3.2 were localized in the chloroplast while transient overexpression of AcLhcb3.1/3.2 in tobacco leaves confirmed a significantly increased content of chlorophyll a. Our findings provide evidence of the characters and evolution patterns of kiwifruit LHCs genes in kiwifruit and verify the AcLhcb3.1/3.2 genes controlling the chlorophyll a content.
Contextualizer: Connecting the Dots of Context with Second-Order Attention
Authors: Maupomé Diego , Meurs Marie-Jean
Published: June 2022

Composing the representation of a sentence from the tokens that it comprises is difficult, because such a representation needs to account for how the words present relate to each other. The Transformer architecture does this by iteratively changing token representations with respect to one another. This has the drawback of requiring computation that grows quadratically with respect to the number of tokens. Furthermore, the scalar attention mechanism used by Transformers requires multiple sets of parameters to operate over different features. The present paper proposes a lighter algorithm for sentence representation with complexity linear in sequence length. This algorithm begins with a presumably erroneous value of a context vector and adjusts this value with respect to the tokens at hand. In order to achieve this, representations of words are built combining their symbolic embedding with a positional encoding into single vectors. The algorithm then iteratively weighs and aggregates these vectors using a second-order attention mechanism, which allows different feature pairs to interact with each other separately. Our models report strong results in several well-known text classification tasks.
Fourth Industrial Revolution between Knowledge Management and Digital Humanities
Authors: Anshari Muhammad , Syafrudin Muhammad , Fitriyani Norma Latif
Published: June 2022

The Fourth Industrial Revolution (4IR) offers optimum productivity and efficiency via automation, expert systems, and artificial intelligence. The Fourth Industrial Revolution deploys smart sensors, Cyber-Physical Systems (CPS), Internet of Things (IoT), Internet of Services (IoS), big data and analytics, Augmented Reality (AR), autonomous robots, additive manufacturing (3D Printing), and cloud computing for optimization purposes. However, the impact of 4IR has brought various changes to digital humanities, mainly in the occupations of people, but also in ethical compliance. It still requires the redefining of the roles of knowledge management (KM) as one of the tools to assist in organization growth, especially in negotiating tasks between machines and people in an organization. Knowledge management is crucial in the development of new digital skills that are governed by the ethical obligations that are necessary in the Fourth Industrial Revolution. The purpose of the study is to examine the role of KM strategies in responding to the emergence of 4IR, its impact on and challenges to the labor market, and employment. This paper also analyzes and further discusses how 4IR and employment issues are being viewed in the context of ethical dilemmas.
Traditional Chinese Medicine Word Representation Model Augmented with Semantic and Grammatical Information
Authors: Ma Yuekun , Sun Zhongyan , Zhang Dezheng , Feng Yechen
Published: June 2022

Text vectorization is the basic work of natural language processing tasks. High-quality vector representation with rich feature information can guarantee the quality of entity recognition and other downstream tasks in the field of traditional Chinese medicine (TCM). The existing word representation models mainly include the shallow models with relatively independent word vectors and the deep pre-training models with strong contextual correlation. Shallow models have simple structures but insufficient extraction of semantic and syntactic information, and deep pre-training models have strong feature extraction ability, but the models have complex structures and large parameter scales. In order to construct a lightweight word representation model with rich contextual semantic information, this paper enhances the shallow word representation model with weak contextual relevance at three levels: the part-of-speech (POS) of the predicted target words, the word order of the text, and the synonymy, antonymy and analogy semantics. In this study, we conducted several experiments in both intrinsic similarity analysis and extrinsic quantitative comparison. The results show that the proposed model achieves state-of-the-art performance compared to the baseline models. In the entity recognition task, the F1 value improved by 4.66% compared to the traditional continuous bag-of-words model (CBOW). The model is a lightweight word representation model, which reduces the training time by 51% compared to the pre-training language model BERT and reduces 89% in terms of memory usage.
State-of-the-Art in Open-Domain Conversational AI: A Survey
Authors: Adewumi Tosin , Liwicki Foteini , Liwicki Marcus
Published: June 2022

We survey SoTA open-domain conversational AI models with the objective of presenting the prevailing challenges that still exist to spur future research. In addition, we provide statistics on the gender of conversational AI in order to guide the ethics discussion surrounding the issue. Open-domain conversational AI models are known to have several challenges, including bland, repetitive responses and performance degradation when prompted with figurative language, among others. First, we provide some background by discussing some topics of interest in conversational AI. We then discuss the method applied to the two investigations carried out that make up this study. The first investigation involves a search for recent SoTA open-domain conversational AI models, while the second involves the search for 100 conversational AI to assess their gender. Results of the survey show that progress has been made with recent SoTA conversational AI, but there are still persistent challenges that need to be solved, and the female gender is more common than the male for conversational AI. One main takeaway is that hybrid models of conversational AI offer more advantages than any single architecture. The key contributions of this survey are (1) the identification of prevailing challenges in SoTA open-domain conversational AI, (2) the rarely held discussion on open-domain conversational AI for low-resource languages, and (3) the discussion about the ethics surrounding the gender of conversational AI.
Movie Box Office Prediction Based on Multi-Model Ensembles
Authors: Ni Yuan , Dong Feixing , Zou Meng , Li Weiping
Published: June 2022

This paper is based on the box office data of films released in China in the past, which was collected from ENDATA on 30 November 2021, providing 5683 pieces of movie data, and enabling the selection of the top 2000 pieces of movie data to be used as the box office prediction dataset. In this paper, some types of Chinese micro-data are used, and a Baidu search of the index data of movie names 30 days before and after the release date, coronavirus disease 2019 (COVID-19) data in China, and other characteristics are introduced, and the stacking algorithm is optimized by adopting a two-layer model architecture. The first layer base learners adopt Extreme Gradient Boosting (XGBoost), the Light Gradient Boosting Machine (LightGBM), Categorical Boosting (CatBoost), the Gradient Boosting Decision Tree (GBDT), random forest (RF), and support vector regression (SVR), and the second layer meta-learner adopts a multiple linear regression model, to establish a box office prediction model with a prediction error, Mean Absolute Percentage Error (MAPE), of 14.49%. In addition, in order to study the impact of the COVID-19 epidemic on the movie box office, based on the data of 187 movies released from January 2020 to November 2021, and combined with a number of data features introduced earlier, this paper uses LightGBM to establish a model. By checking the importance of model features, it is found that the situation of the COVID-19 epidemic at the time of movie release had a certain related impact on the movie box office.
UV Light-Emitting-Diode Traps for Collecting Nocturnal Biting Mosquitoes in Urban Bangkok
Authors: Pimnon Suntorn , Ngoen-Klan Ratchadawan , Sumarnrote Anchana , Chareonviriyaphap Theeraphap
Published: June 2022

Well-designed surveillance systems are required to facilitate a control program for vector-borne diseases. Light traps have long been used to sample large numbers of insect species and are regarded as one of the standard choices for baseline insect surveys. The objective of this study was to evaluate the efficacy of six ultraviolet light-emitting diodes and one fluorescent light for trapping urban nocturnal mosquito species within the Kasetsart University (KU), Bangkok. Ultraviolet light-emitting diodes (UV-LEDs), (LED365, LED375, LED385, LED395, and LED405) and a fluorescent light were randomly assigned to six different locations around the campus in a Latin square design. The traps were operated continuously from 18:00 h to 06:00 h throughout the night. The traps were rotated between six locations for 72 collection-nights during the dry and wet seasons. In total, 6929 adult mosquitoes were caught, with the most predominant genus being Culex, followed by Aedes, Anopheles, Armigeres and Mansonia. Among the Culex species, Culex quinquefasciatus (n = 5121: 73.9%) was the most abundant followed by Culex gelidus (n = 1134: 16.4%) and Culex vishnui (n = 21: 0.3%). Small numbers of Aedes, Armigeres, and Anopheles mosquitoes were trapped [Aedes albopictus (n = 219: 3.2%), Aedes pocilius (n = 137: 2.0%), Armigeres subalbatus (n = 97: 1.4%), Anopheles vagus (n = 70: 1.0%), Aedes aegypti (n = 23: 0.3%)]. There were 2582 specimens (37.2%) captured in fluorescent light traps, whereas 942 (13.6%), 934 (13.5%), 854 (12.3%), 820 (11.8%), and 797 (11.5%) were captured in the LED375, LED405, LED395, LED365, and LED385 traps, respectively. None of the UV-LED light traps were as efficacious for sampling nocturnal mosquito species as the fluorescent light trap. Among the five UV-LED light sources, LED375 trapped the greatest number of mosquitoes. Additional field trials are needed to validate these findings in different settings in order to substantially assess the potential of the LEDs to trap outdoor nocturnal mosquitoes.
First Comprehensive Analysis of Both Mitochondrial Characteristics and Mitogenome-Based Phylogenetics in the Subfamily Eumeninae (Hymenoptera: Vespidae)
Authors: Luo Li , Carpenter James M. , Chen Bin , Li Tingjing
Published: June 2022

The subfamily Eumeninae plays a significant role in the biological control of agricultural pests. However, the characteristics of eumenine mitogenomes that are important molecular markers for phylogenetics are not clearly revealed. Here, 52 eumenine mitogenomes are newly sequenced and annotated, and the phylogenetic relationships of the subfamily are comprehensively analyzed based on 87 vespid mitogenomes. Through the comparative analysis of the 54 eumenine mitogenomes, the gene compositions of about one half of the 54 species match with ancestral insect mitogenome, and remaining others contain two trnM which are highly similar, with 51.86% (Eumenes tripunctatus) to 90.65% (Pseumenes nigripectus) sequence identities, which is unique among the reported mitogenomes of the family Vespidae. Moreover, the translocation trnL1 upstream of nad1 is a common rearrangement event in all eumenine mitogenomes. The results of phylogenetic analyses support the paraphyly of the subfamily Eumeninae and the tribe Odynerini, respectively, and the monophyly of the tribe Eumenini, and verify that the tribe Zethini is a valid subfamily Zethinae. In this study, the relationships between some genera such as Allorhynchium and Pararrhynchium or the taxonomic status of the subgenera such as Eremodynerus and Dirhynchium are found to be confusing and there should be further inquiry with more samples.
First Evidence of CpGV Resistance of Codling Moth in the USA
Authors: Fan Jiangbin , Jehle Johannes A. , Rucker Ann , Nielsen Anne L.
Published: June 2022

Codling moth (Cydia pomonella L.) is a very important pest in apple, pear, and walnut orchards worldwide, including the USA. Cydia pomonella granulovirus (CpGV) is used to control codling moth in organic and conventional production. Due to increasing codling moth infestations from organic apple orchards in Washington State, USA, five codling moth colonies (WA1-WA5) were screened for their susceptibility relative to the isolate GV-0001, the main active ingredient of Cyd-X®, using a discriminating concentration of 6 × 104 OB/mL. Compared to a susceptible laboratory colony, the observed results indicated that GV-0001 lacked efficacy against codling moth colony WA3. It was confirmed that WA3 was the first case of codling moth resistance to CpGV in the USA. Further testing of WA3 was performed on a range of CpGV isolates and a lack of efficacy was observed against additional isolates. However, three newly developed CpGV preparations can efficiently infect larvae from the resistant colony WA3. Our results suggest that there is an urgent need to monitor the situation in the USA, aiming to prevent the emergence or spread of additional codling moth populations with CpGV resistance. Strategies to sustain the efficacy of codling moth control using novel CpGV formulations need to be developed.
Diverse Host Plants of the First Instars of the Invasive Lycorma delicatula: Insights from eDNA Metabarcoding
Authors: McPherson Cameron , Avanesyan Alina , Lamp William O.
Published: June 2022

Identification of host plants of the invasive spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae), has been the focus of many studies. While the adults and late nymphs are relatively easy to observe on plants and to use for molecular gut-content analysis, studying the early instars is more challenging. This study is the continuation of our ongoing efforts to determine the host range for each developmental stage of L. delicatula. In the present study, we focused exclusively on the first nymphal instars, and we used a novel approach, utilizing “bulk” DNA extracts for DNA metabarcoding of nymphal gut contents, to identify all the detectable plants that the nymphs had ingested prior to being collected. We were able to obtain high-quality amplicons (up to 406 bp) of a portion of the rbcL gene and detect 27 unique ingested plant species belonging to 17 families. Both native and introduced plants with the prevalence of trees and grasses were present among the ingested plants. We also identified 13 novel host plants that have not been previously reported for L. delicatula on the U.S. territory. The results from our study have important applications for developing effective programs on early monitoring of invasive L. delicatula.
Abnormal Antennal Olfactory Sensilla Phenotypes Involved in Olfactory Deficit in Bactrocera correcta (Diptera: Tephritidae)
Authors: Guo Kai-Fei , Peng Xiao-Mei , Tu Jie-Yu , Jin Chan , Zhang Wan-Rong , Chen Xi-Zhu , Liu Yong-Jun , Zha Hong-Guang , Shi Wei , Cao Jun
Published: June 2022

The guava fruit fly, Bactrocera correcta, is one of the most destructive pests in the genus Bactrocera and detects environmental odorants mainly through antennal olfactory sensilla phenotypes with nanopores. However, it is unclear whether there are naturally occurring abnormal antennal olfactory sensilla phenotypes that affect olfaction. Here, we found that there were abnormal bulges besides nanopores on the surface of trichoid and basiconic olfactory sensilla in the antennal flagellum of long-term laboratory rearing colony (LTC), and that nanopore number in these olfactory sensilla was also remarkably reduced. Notably, the electroantennogram (EAG) responses of LTC insects to methyl eugenol or β-caryophyllene were inhibited, and their behavioral responses elicited by the same odorants were also impaired. These results revealed naturally occurring abnormal antennal olfactory sensilla phenotypes which were involved in olfactory deficit in B. correcta, providing a platform to further study nanopore-targeted pest control technologies in the future.
Microscopic Identification of Anatomical Elements and Chemical Analysis of Secondary Nests of Vespa velutina nigrithorax du Buyson
Authors: Crespo Nazaret , Louzada José , Fernandes Lisete S. , Tavares Pedro B. , Aranha José
Published: June 2022

Vespa velutina accidentally arrived in Europe (France) in 2004, and rapidly expanded throughout the entire country. Its presence in mainland Portugal was first noticed in 2011. Being an invasive species with no natural predators in the region to control it, it has caused enormous environmental and economic damage, particularly on Apis mellifera (honeybee) colonies. Although there is already some research on this species’ biology, little is known about its adaption to European ecological conditions, specifically in terms of nest building. This type of hornet builds a primary nest in the spring to start a colony. During the summer, they build a secondary nest to develop the main colony. These secondary nests are ovoid-shaped and range in size from 18.7 cm to 45.0 cm in diameter and from 19.2 cm to 65.0 cm in length, attaining their highest development in late summer. The external appearance of these nests is characterized by alternating stripes that are beige and brown in color. The main objective of this study is to identify the composition and the origin of the materials that are used by Vespa velutina nigrithorax to build the outer envelope of these secondary nests. This information could be very interesting and will not only increase our knowledge on the biology of the species in regions far from its original area, but will also be relevant for the future implementation of new policies to control this invasive species by means biological control. Several samples were taken from each nest and were observed under different optical magnifying devices. In the second stage, their chemical composition was analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM/EDS). It was noticed that almost all of the materials used in the nests’ construction were lignocellulose from woody materials from both softwood (gymnosperm) and hardwood (angiosperm) forest species as well from leaves and small particles of agricultural origin (grasses). The beige strips were formed almost exclusively from woody softwood cells, while the brown strips were composed of hardwood cells, leaf tissues, and grasses. Chemically, it was noticed that this material mainly consisted of cellulose, with more than 99% being composed of C and O and very little mineral material from elements such as Na, Al, Si, K, and Ca. The achieved results allow us to state that in the construction of these secondary nests, these hornets only used organic materials that are then probably agglomerated through their mouths.
MDMA for the Treatment of Negative Symptoms in Schizophrenia
Authors: Arnovitz Mitchell D. , Spitzberg Andrew J. , Davani Ashkhan J. , Vadhan Nehal P. , Holland Julie , Kane John M. , Michaels Timothy I.
Published: June 2022

The profound economic burden of schizophrenia is due, in part, to the negative symptoms of the disease, which can severely limit daily functioning. There is much debate in the field regarding their measurement and classification and there are no FDA-approved treatments for negative symptoms despite an abundance of research. 3,4-Methylenedioxy methamphetamine (MDMA) is a schedule I substance that has emerged as a novel therapeutic given its ability to enhance social interactions, generate empathy, and induce a state of metaplasticity in the brain. This review provides a rationale for the use of MDMA in the treatment of negative symptoms by reviewing the literature on negative symptoms, their treatment, MDMA, and MDMA-assisted therapy. It reviews recent evidence that supports the safe and potentially effective use of MDMA to treat negative symptoms and concludes with considerations regarding safety and possible mechanisms of action.
Nintendo Switch Joy-Cons’ Infrared Motion Camera Sensor for Training Manual Dexterity in People with Multiple Sclerosis: A Randomized Controlled Trial
Authors: Cuesta-Gómez Alicia , Martín-Díaz Paloma , Sánchez-Herrera Baeza Patricia , Martínez-Medina Alicia , Ortiz-Comino Carmen , Cano-de-la-Cuerda Roberto
Published: June 2022

Background: The Nintendo Switch® (NS) is the ninth video game console developed by Nintendo®. Joy-Cons® are the primary game controllers for the NS® video game console, and they have an infrared motion camera sensor that allows capturing the patient’s hands without the need to place sensors or devices on the body. The primary aim of the present study was to evaluate the effects of the NS®, combined with a conventional intervention, for improving upper limb (UL) grip muscle strength, coordination, speed of movements, fine and gross dexterity, functionality, quality of life, and executive function in multiple sclerosis (MS) patients. Furthermore, we sought to assess satisfaction and compliance levels. Methods: A single-blinded, randomized clinical trial was conducted. The sample was randomized into two groups: an experimental group who received treatment based on Dr Kawashima’s Brain Training® for the NS® (20 min) plus conventional rehabilitation (40 min), and a control group who received the same conventional rehabilitation (60 min) for the ULs. Both groups received two 60 min sessions per week over an eight-week period. Grip strength, the Box and Blocks Test (BBT), the Nine Hole Peg Test (NHPT), the QuickDASH, the Multiple Sclerosis Impact Scale (MSIS-29), the Trail Making Test (TMT), and the Stroop Color and Word Test (SCWT) were used pre- and post-treatment. Side effects and attendance rates were also recorded. Results: Intragroup analysis showed significant improvements for the experimental group in the post-treatment assessments for grip strength in the more affected side (p = 0.033), the BBT for the more (p = 0.030) and the less affected side (p = 0.022), the TMT (A section) (p = 0.012), and the QuickDASH (p = 0.017). No differences were observed for the control group in intragroup analysis, but they were observed in the NHPT for the more affected side (p = 0.012). The intergroup analysis did not show differences between both groups. Conclusions: Our results show that an eight-week experimental protocol, after using Dr Kawashima’s Brain Training® and the right-side Joy-Con controller for the NS®, combined with a conventional intervention, showed improvements in grip strength, coordination, fine and gross motor function, executive functions, and upper limb functionality in the experimental group. However, no differences were observed when both groups were compared in the intergroup analysis. The addition of Brain Training® for the NS® for the upper limb rehabilitation did not show side effects and was rated with a high satisfaction and excellent compliance in people with MS. Trial registration: This randomized controlled trial has been registered at ClinicalTrials Identifier: NCT04171908, November 2019.
Interventional Radiology in the Management of Metastases and Bone Tumors
Authors: Sgalambro Ferruccio , Zugaro Luigi , Bruno Federico , Palumbo Pierpaolo , Salducca Nicola , Zoccali Carmine , Barile Antonio , Masciocchi Carlo , Arrigoni Francesco
Published: June 2022

Interventional Radiology (IR) has experienced an exponential growth in recent years. Technological advances of the last decades have made it possible to use new treatments on a larger scale, with good results in terms of safety and effectiveness. In musculoskeletal field, painful bone metastases are the most common target of IR palliative treatments; however, in selected cases of bone metastases, IR may play a curative role, also in combination with other techniques (surgery, radiation and oncology therapies, etc.). Primary malignant bone tumors are extremely rare compared with secondary bone lesions: osteosarcoma, Ewing sarcoma, and chondrosarcoma are the most common; however, the role of interventional radiology in this fiels is marginal. In this review, the main techniques used in interventional radiology were examined, and advantages and limitations illustrated. Techniques of ablation (Radiofrequency, Microwaves, Cryoablation as also magnetic resonance imaging-guided high-intensity focused ultrasound), embolization, and Cementoplasty will be described. The techniques of ablation work by destruction of pathological tissue by thermal energy (by an increase of temperature up to 90 °C with the exception of the Cryoablation that works by freezing the tissue up to −40 °C). Embolization creates an ischemic necrosis by the occlusion of the arterial vessels that feed the tumor. Finally, cementoplasty has the aim of strengthening bone segment weakened by the growth of pathological tissue through the injection of cement. The results of the treatments performed so far were also assessed and presented focused the attention on the management of bone metastasis.
Clinical Time Delay Distributions of COVID-19 in 2020–2022 in the Republic of Korea: Inferences from a Nationwide Database Analysis
Authors: Shim Eunha , Choi Wongyeong , Song Youngji
Published: June 2022

Epidemiological distributions of the coronavirus disease 2019 (COVID-19), including the intervals from symptom onset to diagnosis, reporting, or death, are important for developing effective disease-control strategies. COVID-19 case data (from 19 January 2020 to 10 January 2022) from a national database maintained by the Korea Disease Control and Prevention Agency and the Central Disease Control Headquarters were analyzed. A joint Bayesian subnational model with partial pooling was used and yielded probability distribution models of key epidemiological distributions in Korea. Serial intervals from before and during the Delta variant’s predominance were estimated. Although the mean symptom-onset-to-report interval was 3.2 days at the national level, it varied across different regions (2.9–4.0 days). Gamma distribution showed the best fit for the onset-to-death interval (with heterogeneity in age, sex, and comorbidities) and the reporting-to-death interval. Log-normal distribution was optimal for ascertaining the onset-to-diagnosis and onset-to-report intervals. Serial interval (days) was shorter before the Delta variant-induced outbreaks than during the Delta variant’s predominance (4.4 vs. 5.2 days), indicating the higher transmission potential of the Delta variant. The identified heterogeneity in region-, age-, sex-, and period-based distributions of the transmission dynamics of COVID-19 will facilitate the development of effective interventions and disease-control strategies.