bluuebunny/crossref_metadata_2025 huggingface dataset:
Dilemmas of nudging in public health: an ethical analysis of a Danish pamphlet
Authors: Busch Jacob , Madsen Emilie Kirstine , Fage-Butler Antoinette Mary , Kjær Marianne , Ledderer Loni
Published: November 2020

Nudging has been discussed in the context of public health, and ethical issues raised by nudging in public health contexts have been highlighted. In this article, we first identify types of nudging approaches and techniques that have been used in screening programmes, and ethical issues that have been associated with nudging: paternalism, limited autonomy and manipulation. We then identify nudging techniques used in a pamphlet developed for the Danish National Screening Program for Colorectal Cancer. These include framing, default nudge, use of hassle bias, authority nudge and priming. The pamphlet and the very offering of a screening programme can in themselves be considered nudges. Whether nudging strategies are ethically problematic depend on whether they are categorized as educative- or non-educative nudges. Educative nudges seek to affect people’s choice making by engaging their reflective capabilities. Non-educative nudges work by circumventing people’s reflective capabilities. Information materials are, on the face of it, meant to engage citizens’ reflective capacities. Recipients are likely to receive information materials with this expectation, and thus not expect to be affected in other ways. Non-educative nudges may therefore be particularly problematic in the context of information on screening, also as participating in screening does not always benefit the individual.
Chronic kidney disease stage stratifies short- and long-term outcomes after aortic root replacement
Authors: Yamabe Tsuyoshi , Zhao Yanling , Kurlansky Paul A , Nitta Suzuka , Kelebeyev Saveliy , Bethancourt Casidhe-Nicole R , George Isaac , Smith Craig R , Takayama Hiroo
Published: November 2020

OBJECTIVES

Chronic kidney disease (CKD) is prevalent in patients undergoing cardiovascular surgery, and it negatively impacts procedural outcomes; however, its influence on the outcomes of aortic surgery has not been well studied. This study aims to elucidate the importance of CKD on the outcomes of aortic root replacement (ARR).

METHODS

Patients who underwent ARR between 2005 and 2019 were retrospectively reviewed (n = 882). Patients were divided into 3 groups based on the Kidney Disease: Improving Global Outcomes criteria: Group 1 [estimated glomerular filtration rate (eGFR) ≥ 60 ml/min/1.73 m2, n = 421); Group 2 (eGFR = 30–59 ml/min/1.73 m2, n = 424); and Group 3 (eGFR < 30 ml/min/1.73 m2, n = 37). To reduce potential confounding, a propensity score matching was also performed between Group 1 and the combined group of Group 2 and Group 3. The primary end point was 10-year survival. Secondary end points were in-hospital mortality and perioperative morbidity.

RESULTS

Severe CKD patients presented with more advanced overall chronic and acute illnesses. Kaplan–Meier analysis showed a significant correlation between CKD stage and 10-year survival (log-rank P < 0.001). The number of events for Group 1 was 15, Group 2 was 49 and Group 3 was 11 in 10 years. Group 3 had significantly higher in-hospital mortality (13.5% vs 3.5% in Group 2 vs 0.7% in Group 1, P < 0.001) and stroke (8.1% vs 7.1% vs 1.2%, P < 0.001) as well as introduction to new dialysis (27.0% vs 5.4% vs 1.7%, P < 0.001). eGFR was shown to be an independent predictor of mortality (hazard ratio, 0.98; 95% confidence interval, 0.96–0.99). Comparison between propensity matched groups showed similar postoperative outcomes, and eGFR was still identified as a predictor of mortality (hazard ratio, 0.97; 95% confidence interval, 0.95–0.99).

CONCLUSIONS

Higher stage in CKD negatively impacts the long-term survival in patients who are undergoing ARR.
Backward Shift and Nearly Invariant Subspaces of Fock-type Spaces
Authors: Aleman Alexandru , Baranov Anton , Belov Yurii , Hedenmalm Haakan
Published: November 2020

We study the structure of the backward shift invariant and nearly invariant subspaces in weighted Fock-type spaces ${\mathcal{F}}_W^p$, whose weight is not necessarily radial. We show that in the spaces ${\mathcal{F}}_W^p$, which contain the polynomials as a dense subspace (in particular, in the radial case), all nontrivial backward shift invariant subspaces are of the form $\mathcal{P}_n$, that is, finite-dimensional subspaces consisting of polynomials of degree at most $n$. In general, the structure of the nearly invariant subspaces is more complicated. In the case of spaces of slow growth (up to zero exponential type), we establish an analogue of de Branges’ ordering theorem. We then construct examples that show that the result fails for general Fock-type spaces of larger growth.
Suppression of liver transplant rejection by anti-donor MHC antibodies via depletion of donor immunogenic dendritic cells
Authors: Ueta Hisashi , Xu Xue-Dong , Yu Bin , Kitazawa Yusuke , Yu Enqiao , Hara Yoshiaki , Morita-Nakagawa Miwa , Zhou Shu , Sawanobori Yasushi , Ueha Satoshi , Rokutan Kazuhito , Tanaka Toshiya , Tokuda Nobuko , Matsushima Kouji , Matsuno Kenjiro
Published: November 2020

Background

We previously found two distinct passenger dendritic cell (DC) subsets in the rat liver that played a central role in the liver transplant rejection. In addition, a tolerance-inducing protocol, donor-specific transfusion (DST), triggered systemic polytopical production of depleting alloantibodies to donor class I MHC (MHCI) antigen (DST-antibodies).

Methods

We examined the role of DST-antibodies in the trafficking of graft DC subsets and the alloresponses in a rat model. We also examined an anti-donor class II MHC (MHCII) antibody that recognizes donor DCs more selectively.

Results

Preoperative transfer of DST-antibodies or DST pretreatment eliminated all passenger leukocytes, including both DC subsets and depleted the sessile DCs in the graft to ~20% of control. The CD172a+CD11b/c+ immunogenic subset was almost abolished. The intrahost direct or semi-direct allorecognition pathway was successfully blocked, leading to a significant suppression of the CD8+ T-cell response in the recipient lymphoid organs and the graft with delayed graft rejection. Anti-donor MHCII antibody had similar effects without temporary graft damage. Although DST pretreatment had a priming effect on the proliferative response of recipient regulatory T cells, DST-primed sera and the anti-donor MHCII antibody did not.

Conclusion

DST-antibodies and anti-donor MHCII antibodies could suppress the CD8+ T-cell-mediated liver transplant rejection by depleting donor immunogenic DCs, blocking the direct or semi-direct pathways of allorecognition. Donor MHCII-specific antibodies may be applicable as a selective suppressant of anti-donor immunity for clinical liver transplantation without the cellular damage of donor MHCII– graft cells and recipient cells.
Clinician involvement in research on machine learning–based predictive clinical decision support for the hospital setting: A scoping review
Authors: Schwartz Jessica M , Moy Amanda J , Rossetti Sarah C , Elhadad Noémie , Cato Kenrick D
Published: November 2020

Objective

The study sought to describe the prevalence and nature of clinical expert involvement in the development, evaluation, and implementation of clinical decision support systems (CDSSs) that utilize machine learning to analyze electronic health record data to assist nurses and physicians in prognostic and treatment decision making (ie, predictive CDSSs) in the hospital.

Materials and Methods

A systematic search of PubMed, CINAHL, and IEEE Xplore and hand-searching of relevant conference proceedings were conducted to identify eligible articles. Empirical studies of predictive CDSSs using electronic health record data for nurses or physicians in the hospital setting published in the last 5 years in peer-reviewed journals or conference proceedings were eligible for synthesis. Data from eligible studies regarding clinician involvement, stage in system design, predictive CDSS intention, and target clinician were charted and summarized.

Results

Eighty studies met eligibility criteria. Clinical expert involvement was most prevalent at the beginning and late stages of system design. Most articles (95%) described developing and evaluating machine learning models, 28% of which described involving clinical experts, with nearly half functioning to verify the clinical correctness or relevance of the model (47%).

Discussion

Involvement of clinical experts in predictive CDSS design should be explicitly reported in publications and evaluated for the potential to overcome predictive CDSS adoption challenges.

Conclusions

If present, clinical expert involvement is most prevalent when predictive CDSS specifications are made or when system implementations are evaluated. However, clinical experts are less prevalent in developmental stages to verify clinical correctness, select model features, preprocess data, or serve as a gold standard.
84 Effect of rearing cross-fostered piglets in litters of either uniform or mixed birth weights on pre-weaning performance
Authors: Vande Pol Katherine , Bautista Ovidio , Harper Heath , Ellis Michael , Shull Caleb M , Brown Katie
Published: November 2020

There is limited understanding of optimum cross-fostering methods to use to maximize piglet performance. This study evaluated effects of within-litter birth weight variation after cross-fostering on pre-weaning piglet removals (PR; morbidity and mortality) and ADG. A hierarchical incomplete block design was used (blocking factors day of farrowing and sow parity and structure) with a 3x2 factorial arrangement of treatments: 1) Birth Weight Category (BWC): Light (< 1.0 kg), Medium (1.0-1.5 kg), or Heavy (1.5-2.0 kg); 2) Litter Composition (LC): Uniform (piglets of the same BWC), and Mixed (equal numbers of piglets from each BWC). Piglets were weighed 24 h after birth and allotted to form litters of 15 cross-fostered piglets. The experimental unit was 5 piglets of the same BWC (3 experimental units per litter). A total of 102 litters were allotted to 17 blocks of 6 litters (1 Uniform litter of each BWC; 3 Mixed litters) with 51 replicates (3 replicates/block of 6 litters). Weaning weights and PR were measured. PROC GLIMMIX and MIXED of SAS were used to analyze PR and other data, respectively. Models included BWC, LC, the interaction, and replicate within block. There were treatment interactions (P < 0.05) for all measures except birth weight. There was no effect (P > 0.05) of LC on weaning weight or ADG for Light or Medium piglets; Heavy piglets had greater (P < 0.05) weaning weight and ADG in Mixed than in Uniform litters. PR were greater (P < 0.05) for Light piglets in Mixed than in Uniform litters, and for Heavy piglets in Uniform than in Mixed litters. PR for Medium piglets were similar (P > 0.05) across LC treatments. In conclusion, rearing cross-fostered piglets in Uniform litters reduced PR for Light piglets, but increased PR and reduced ADG of Heavy piglets, with no effect for Medium piglets.
81 Effects of NeutraPath™ on growth performance, diarrhea and fecal-hemolytic coliforms of weaned pigs challenged with an enterotoxigenic Escherichia coli
Authors: Johnston LeAnn , Ji Peng , Xue Hongyu
Published: November 2020

This study was aimed to investigate NeutraPath’s effects on performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs infected with enterotoxigenic E. coli (ETEC). NeutraPath, a blend of essential oils, fatty acids and toxin-adsorbing minerals has bacteriostatic/bactericidal effects. Thirty-six weanling pigs (21 d old; average BW 6.88 kg; 18 barrows, 18 gilts) blocked by weight and gender, were assigned to one of three dietary treatments in an RCB (1 pig/pen; 12 replicates/treatment). Treatments were control or dietary supplementation with 0.25% or 0.50% NeutraPath. This study lasted 28 d with 7 d before and 21 d after the first E. coli inoculation (d 0). All pigs were orally inoculated with 1010 cfu F18+ ETEC/3-mL dose for 3 consecutive d. Growth performance data was obtained on d 0, d 7, d 14 and d 21 post-inoculation (PI). On d 2 PI, fecal samples were obtained and plated on blood and MacConkey agars to determine total coliforms and β-hemolytic coliforms. Treatment effects were analyzed using one-way ANOVA or repeated measures ANOVA followed by post hoc Tukey’s test. The 0.5% NeutraPath treatment resulted in a 12.7% and a 14.0% increase (P < 0.05) in BW (relative to initial BW at d 0 PI) at d 7 PI and d 21 PI, respectively. Pigs fed either NeutraPath dose had greater overall growth during the 28-d period (P< 0.05) producing improvements in feed efficiency during d 14-21 PI (P< 0.05). Feeding 0.25% NeutraPath improved (P < 0.05) diarrhea kinetics d 0-11 PI, Diarrhea score (DS) area under the curve for d 0-11 and d 0-21 PI, moderate (DS≥3) and severe (DS≥4) diarrhea incidence and tended (P=0.08) to lower the percentage of fecal β-hemolytic coliforms compared to control. In conclusion, NeutraPath supplementation can enhance growth performance and decrease incidence of severe diarrhea in weaned pigs challenged with F18+ ETEC.
243 Effects of rubber matting on cattle performance and carcass characteristics in slatted facilities
Authors: Dawson Cody , Henley Parker , Schroeder Adam , Hayes Courtney , Felix Tara , Shike Daniel W , McCann Joshua C
Published: November 2020

The objective was to determine effects of interlocking rubber floor matting in slatted indoor cattle feeding facilities on cattle performance and carcass characteristics. In experiment 1, Fall-born Angus x Simmental steers (N = 206; BW = 228 ± 34 kg) were blocked by weight and assigned to 32 pens. Pens were randomly assigned to 1 of 4 treatments: new Max Grip Animat matting (MG), new Animat Pebble matting (PEB), old Animat Pebble matting (OLD), and no matting/concrete slating (CONC). Steers were fed a common diet for 209 d with an average stocking density of 3.70 m2 per steer. Final BW was affected (P = 0.02) by treatment with PEB steers being the heaviest, MG and CONC were intermediate, and OLD was the lightest. Treatment affected (P < 0.01) ADG with PEB steers being greatest, MG and CONC were intermediate, and OLD was the least. Flooring treatment did not affect overall dry matter intake (P = 0.16) or G:F (P = 0.94). Flooring treatment did not affect (P ≥ 0.19) any carcass traits. In experiment 2, Fall-born Angus x Simmental steers (N = 189; BW = 352 ± 43 kg) were blocked by weight and assigned to 21 pens. Pens were randomly assigned to 1 of 3 treatments: new Animat Pebble matting (PEB2), old Animat Pebble matting (OLD2), or no matting/concrete slating (CON2). Steers were fed a common diet for 112 d with an average stocking density of 2.64 m2 per steer. After 112 days on feed, flooring treatment did not affect (P ≥ 0.30) BW or ADG. There were no treatment differences (P = 0.13) observed for overall DMI at d 112. Overall, results suggest new rubber matting in slatted indoor cattle feeding facilities improved ADG during a 209 d trial.
241 Trends in the percentage of modified live, killed, and combination respiratory viral vaccines administered to beef calves offered for sale in summer video auctions from 2000 through 2018
Authors: Smith Maggie J , King Mike E , Fike Karol E , McCabe Esther D , Rogers Glenn M , Odde Kenneth G
Published: November 2020

The objective of this study was to identify trends in the percentage of type of respiratory viral vaccines administered to lots of beef calves offered for sale in summer video auctions from 2000 through 2018. There were 59,762 lots of single-gender beef calves (7,167,352 total calves) offered for sale in 145 summer video auctions during these years. Information describing calf lots was obtained from the auction service (Superior Livestock Auction, Fort Worth, TX) which included named vaccines administered to the lot. Named 4- or 5-way respiratory viral vaccines were classified into three groups based on the type of antigens they contained: all modified live antigens (MLV), all killed antigens (KILLED), and a combination of modified live and killed antigens (COMBO). The Cochran-Armitage Trend Test was used to quantify the significance of a trend in the usage of each respiratory viral vaccine type. There was an increase (P < 0.0001) in the percentage of MLV vaccines given to beef calf lots from 2000 (39.7%) through 2018 (88.9%). At the same time, the percentages of both KILLED and COMBO vaccines administered to lots of beef calves declined (P < 0.0001 and P < 0.0001, respectively). In 2000, 31.2% and 29.1% of the total respiratory viral vaccines given to beef calf lots were KILLED or COMBO vaccines, respectively. By 2018, only 4.7% of respiratory viral vaccines were KILLED, and only 6.4% were COMBO vaccines. This dramatic shift indicates an industry trend towards increasing MLV vaccine utilization compared with declining usage of KILLED and COMBO vaccines. This trend may be a result of MLV vaccine approval for use in calves nursing pregnant cows.
205 An Adjusted SID-Thr:Lys Ratio Offsets Growth Performance Depressions in Finishing Pigs Fed Moderately High Distillers Dried Grains with Solubles (DDGS)
Authors: Norton Sharon A , Xue Pengcheng , Ramer Brian , Erickson Jim
Published: November 2020

Two experiments were conducted to determine the impact of dietary SID Thr:Lys ratio (THR) on the performance of grow-finishing pigs. In Experiment 1, a total of 1,152 pigs (initial BW = 21.0 ± 1.4 kg; 12 pens/treatment; 24 pigs/pen) were used in a randomized complete blocked design, with a 2 x 2 factorial arrangement of treatments containing 2 levels of DDGS (0 vs 25%) and 2 levels of THR (Low vs. High, 0.60 vs 0.67). Equal energy and nutrient levels that met or exceeded NRC (2012) requirements were achieved across treatments. A total of 1,056 pigs (initial BW=18.6 ± 1.9 kg; 16 pens/treatment; 22 pig/pen) were used in Experiment 2 with the same experimental design, except for the SID Thr:Lys ratios were formulated to 0.60, 0.60, 0.61, 0.62 and 0.63 in 5 dietary phases for Low THR, and +0.03 for all phases in High THR. The MIXED procedures were employed for statistical analysis. Orthogonal contrasts were applied to evaluate main effects of DDGS, THR, and their interactions. In the results, Experiment 1 showed from 20 to 60 kg of BW 25% DDGS inclusion reduced (P < 0.01) FBW (63.4 vs 61.2 kg), ADG (0.88 vs 0.84 kg/d), and G:F (0.50 vs 0.49). There was an interaction between DDGS and THR on G:F (P < 0.01), where High THR improved G:F in diets containing 25% DDGS. In Experiment 2, reduced ADG (0.88 vs 0.81 kg/d) and G:F (0.53 vs 0.51) were observed for pigs fed 25% DDGS in grower phase (P < 0.01). In the early finisher, increased THR in diets with 25% DDGS improved (P < 0.01) FBW (90.54 vs 94.12 kg), ADG (0.95 vs 1.07 kg/d), and tended to improve G:F (P < 0.10; 0.37 vs 0.41), but not in pigs fed 0% DDGS. In summary, these two studies demonstrated greater SID Thr:Lys ratio may help offset the negative impact of fibrous diets on BW gain and feed efficiency in grow-finishing pigs.
15 Young Scholar Presentation: Impact of a Near Ideal Amino Acid Profile on the Efficiency of Nitrogen and Energy Utilization in Lactating Sows
Authors: Zhang Sai , Trottier Nathalie
Published: November 2020

The objective was to determine the efficiency of EAA and energy for lactation in sows and quantify their metabolic heat production fed a reduced protein diet. We hypothesized that feeding a reduced protein diet with near ideal AA profile (NIAA) and a leucine:lysine of 1.14 improves the dietary essential AA (EAA) and energy utilization efficiency and reduces the metabolic heat associated with lactation, compared to feeding diets containing leucine:lysine of 1.63. Three diets were formulated iso-calorically (2,580 kcal/kg net energy), including 1) control diet with a 1.63 leucine:lysine (CON; 18.75% CP), 2) optimal diet with NIAA profile and 1.14 leucine:lysine (OPT; 13.75% CP) by supplementing crystalline AA to minimum requirements; and 3) OPT diet with L-Leucine (Leu) supplementation to achieve CON Leu:Lys of 1.63 (OPTLEU; 14.25% CP). Experiment 1 was to estimate maximal biological efficiency value (MBEV) of EAA in lactating sows fed CON, OPT and OPTLEU diets. Feeding OPT diet improved efficiency of nitrogen (N) (79.1%), arginine (61.1%), His (78.3%), Ile (65.4%), Leu (75.1%), Met + Cys (78.2%), Phe (53.4%), Phe + Tyr (69.5%) and Trp (70.1%) and maximized the efficiency of Lys (63.2%), Met (67.9%), Thr (71.0%) and Val (57.0%) for milk production over a 21-day lactation. Experiment 2 was to estimate dietary energetic efficiency, energy partitioning and heat production in lactating sows fed CON, OPT and OPTLEU diets. Feeding OPT led to greater energy utilization for lactation due to less urinary energy and metabolic heat loss, and triggered dietary energy deposition into milk at the expense of maternal lipid mobilization. Feeding OPTLEU compared to OPT reduced energy utilization for lactation by directing dietary energy away from the mammary gland and towards maternal pool, in part explaining the efficacy of a NIAA diet over CON. Experiment 3 was to measure heat production in lactating sows fed CON and OPT diets and exposed to thermal neutral and HS environments. Sows fed OPT diet produced less metabolic heat and had lower body temperature when exposed to HS conditions compared to CON fed sows. In conclusion, feeding a diet with NIAA profile containing Leu:Lys of 1.14 improves EAA and energy utilization efficiency for lactation, and reduces the metabolic heat associated with lactation compared to feeding a diet with Leu:Lys of 1.63.
109 Stretching forages with limited quality and quantity by using non-forage fiber sources (NFFS) available in the Midwestern United States
Authors: Kononoff Paul J , Buse Kassidy
Published: November 2020

Last year, over 110 million metric tons of corn silage and 2.7 million metric tons of alfalfa were harvested (USDA, 2018). Despite these amounts and because forage is costly to transport, individual dairy producers who experience troubling seeding, growing, and/or harvest may be faced with the challenge of maintaining normal milk production by feeding less forage. Fortunately, non-forage fiber sources (NFFS) may be used to supply nutrients that would otherwise come from forages. Furthermore, many of these feeds, such are corn gluten feed, beet pulp, brewers grains, canola meal, distillers grains and solubles, and soyhulls are plentifully in the Midwestern United States. Although each NFFS is unique and the chemical composition may vary due to source, when making ration balancing decisions to include them, nutritionists may be faced with similar challenges. These challenges include that these feeds are low in effective fiber, many contain less fiber than forages, are higher in rumen undegradable protein, may vary in chemical composition, and may include toxins or antinutritional factors. Although the modern dairy cow is adaptable and can utilize use NFFS as sources of nutrients to produce milk, there are still limitations to how they can be included in diets.
96 Estimation of the genetic parameters of beef bull semen attributes
Authors: Butler Madison L , Bormann Jennifer M , Weaber Robert L , Grieger David M , Rolf Megan M
Published: November 2020

Fertility is a critical factor in cattle production because it directly relates to producing offspring to offset production costs. A number of semen attributes are believed to affect fertility and are frequently measured as part of routine breeding soundness exams or semen collection procedures. The objective of this study was to estimate the variance components for different semen quantity and quality traits that may influence fertility using phenotypes collected on 369 Simmental bulls. A total of 7,436 bull collection records including volume and concentration were obtained from two bull studs and evaluated utilizing a linear univariate animal model with repeated records. The five-generation pedigree used in the analysis consisted of 3,336 sires and 7,225 dams. Volume is the total amount of ejaculate and measured as milliliters of total ejaculate. Volume measurements ranged from 0.100 to 41.30 milliliters, with an average of 7.599 milliliters. Concentration is a measurement of millions of spermatozoa per milliliter. Concentration measurements ranged from 10 to 3,651 with an average of 1,053 million spermatozoa per milliliter. Fixed effects were included in the model if the effect was significant (P ≤ 0.05) for either backward or forward selection. Fixed effects included bull owner, collection center, location within center, collection day within year as a Julian day, collection year fit as class variables. Fixed effects fit as covariates included age of bull at collection (linear and quadratic), days since previous collection (linear and quadratic), and scrotal circumference (quadratic). Heritability estimates of volume and concentration were 0.43 ± 0.14 and 0.40 ± 0.15. These moderate heritability estimates indicate genetic improvement can be made in beef bull semen quality traits through selection.
18 Impact of in-feed vs. in-water antibiotic administrations on the fecal prevalence and antimicrobial susceptibilities of Campylobacter and Salmonella in piglets
Authors: Ishengoma Victor L , Amachawadi Raghavendra G , Habib Kellen A , Shi Xiaorong , Mahmood Taghreed , Hutchens Wade M , Tokach Mike D , Dritz Steve S , Woodworth Jason C , Goodband Robert D , DeRouchey Joel M , Apley Michael D , Nagaraja T G
Published: November 2020

Campylobacter and Salmonella are common food borne pathogens in the gut of pigs and are shed in the feces. The control of these bacteria in pigs is of importance in reducing the potential for transmission to humans. In swine, oral route, either in-feed or in-water, is by far the most common route of administration of antibiotics. Because the distribution of the antibiotic in the gut and the dosage are different, the impact of in-feed vs. in-water administration of antibiotics on the fecal shedding of food borne pathogens, Campylobacter and Salmonella, and on the development of antimicrobial resistance (AMR) in gut bacteria is a largely unexplored area. Therefore, a study was conducted to compare the effects of in-feed and in-water antibiotic administration on fecal prevalence of Campylobacter and Salmonella and AMR development in nursery piglets. A total of 1,296 weaned piglets were allocated into pens (48 pens; 27 pigs per pen) distributed in a single room. Pens were assigned randomly to six treatment groups; Control (No antibiotic), In-feed chlortetracycline (CTC), In-water CTC, In-feed tiamulin, In-water tiamulin, and a combination of CTC and tiamulin (In-feed). Fresh fecal samples were collected randomly from 5 of 27 piglets from each pen on pre-treatment (days -7, 0), treatment (days 7, 14) and post-treatment (days 21, 28) phases. Bacterial isolations and identifications were done by culture method and PCR, respectively. Overall prevalence of Campylobacter and Salmonella were 18.2% (262/1,440) and 3.9% (56/1,440) respectively. Speciation of Campylobacter isolates indicated C. hyointestinalis (17.9%; 258/1,440) and C. coli (0.3%; 4/1,440). Pigs from control group had a higher prevalence (P< 0.05) of both Campylobacter and Salmonella when compared to other treatment groups. Both treatment and post-treatment phases had a significant effect on the prevalence of Campylobacter and Salmonella (P< 0.05).
21 Effects of supplementing late gestation sow diets with zinc on pre-weaning mortality of pigs under commercial rearing conditions
Authors: Holen Julia P , Urriola Pedro E , Schwartz Mark , Jang Jae-Cheol , Shurson Gerald C , Johnston Lee
Published: November 2020

The objective of this experiment was to determine pre-weaning survival of pigs when sows were supplemented with 3 dietary levels of Zn in late gestation. Gilts and sows (n = 339) were assigned to one of three dietary treatments based on parity. Treatments were: 1) Control – corn-soybean meal-based diet containing 125 ppm supplemental Zn as ZnSO4 (75 ppm Zn) and AvailaZn™ (50 ppm Zn, CON); 2) Intermediate – as Control + 240 ppm supplemental Zn as ZnSO4 (INT); and 3) High – as Control + 470 ppm supplemental Zn as ZnSO4 (HI). Final supplemental Zn concentrations of diets were: 1) CON – 125 ppm; 2) INT – 365 ppm; and 3) HI – 595 ppm. Sows received dietary treatments from about d 85 of gestation until farrowing. Individual piglet birth weights were recorded within 12 h of parturition and all instances of piglet mortality were recorded. The statistical model considered fixed effects of diet and random effects of parity. Chi-square analysis was used to determine diet effects on piglet mortality. There were no differences in total pigs born, born alive, or weaned per litter. However, piglets from sows fed the INT diet had heavier (P < 0.05) birth weights (1.42 kg) than those fed CON (1.38 kg). Furthermore, incidence of low birth weight pigs was less (P < 0.05) for sows consuming INT compared with sows fed CON and HI (Table 1). Overall piglet mortality tended to decrease (P < 0.10) as dietary Zn increased. Furthermore, mortality of low birth weight pigs decreased (P < 0.05) as dietary Zn increased. Overall, effects of supplemental dietary Zn at 365 and 595 ppm in late gestation improved pre-weaning survival of low birth weight piglets and reduced overall pre-weaning mortality of piglets.
19 Assessment of Nitrogen Intake, Excretion and Deposition in lipopolysaccharide-Challenged Pigs
Authors: Barcellos Joyce , Alves Warley J , Ferreira Carolaine R , Monteiro Filipe , Viana Gabriel , Campos Paulo H Reis Furtado , Silva Fabyano , Hannas Melissa I
Published: November 2020

This study was conducted to assess the effect of E. Coli lipopolyssacharide challenge (IC) on Nitrogen Balance (NB) in growing pigs (19 kg). Three NB trials were performed in a complete RBD, with six nitrogen levels (NL), two sanitary status (control and IC) and two individual replicates per treatment (6 x 2 x 6). Pigs were intramuscular injected with 30 and 34 μg LPS/kg for two consecutive days. Dietary NL ranged from 1.06 to 4.48 % (dry matter), with lysine being limiting in all diets. NB trials lasted 11 days in which feces and urine were collected at the last four days to determine N intake (NI), N excretion (NEX) and N deposition (ND). Data were analyzed as two-way ANOVA and polynomial contrasts were used. Body weight were unaffected by the factors under study (P >0,05). LPS-challenged pigs had a lower (P< 0,05) feed intake compared with control group (372,98 vs. 417,82). Interaction between NL and IC were observed (P< 0,05) for NI, NEX and ND. NL linearly increased NI, NEX and ND, with different equations for both groups (P< 0,05): ND = 127,86 + 246,95 NL in control group whereas ND = 150,66 + 155,4 in LPS group. LPS-challenged pigs fed the three highest NL exhibited a decrease in NI compared with control group (P< 0,05). From NL2 to NL6, ND was also impaired in LPS-challenged pigs compared with control. Nitrogen excretion was higher in LPS-challenged pigs fed the two highest NL compared with pigs from control group (P< 0,05). In conclusion, LPS challenge impairs nitrogen retention in growing pigs by 37%. These outcomes can be used further for modelling procedures to estimate lysine requirements of pigs in different sanitary status.
36 Evaluation and mitigation of the effects of in utero heat stress on pig performance following weaning and transport
Authors: Maskal Jacob M , Duttlinger Alan W , Kpodo Kouassi R , McConn Betty R , Byrd Christopher , Richert Brian T , Lay Donald , Marchant-Forde Jeremy , Lucy Matthew , Safranski Tim , Johnson Jay S
Published: November 2020

In utero heat stress (IUHS) negatively affects swine productivity and is a growing concern for the swine industry. The study objective was to evaluate and mitigate the negative effects of IUHS on pig performance following weaning and transport. Based on previous research, we hypothesized that IUHS pigs would have reduced performance relative to in utero thermoneutral (IUTN) pigs due to greater maintenance costs and supplementing a nutrient-dense (ND) diet would improve IUHS pig performance to similar levels as IUTN pigs fed a common (C) diet. Therefore, 24 bred gilts were assigned to either thermoneutral (TN; n = 12; 17.5 ± 2.1°C) or heat stress (HS; n = 12; cyclical 27°C to 37°C) conditions for the first half of gestation (d1 to 58) and then TN conditions for the remainder of gestation. At weaning (16.2 ± 0.4 d), pigs (N = 160; 4.78 ± 0.15 kg BW) were transported (loading + transport + unloading) for approximately 12h. Pigs were then blocked into pens (n = 4 pigs/pen) by in utero and dietary treatments which were fed for 14 d (Period-1): IUTN-C (n = 10 pens), IUTN-ND (n = 10 pens), IUHS-C (n = 10 pens), IUHS-ND (n = 10 pens). Following Period-1, all pigs were fed the C diet from d15 to 35 post-weaning and transport (Period-2). Production measures were taken on d 0, 7, 14, 21, 28, and 35 post-weaning and transport to calculate ADG, ADFI, and G:F. During Period-1, ADG was reduced overall (P = 0.03, 10.6%) for IUHS pigs compared to IUTN pigs. Pigs fed ND diets had reduced ADFI during Period-1 (P = 0.02, 8.8%) relative to C fed pigs, regardless of in utero treatment. During Period-2, ADFI was increased (P = 0.03, 4.7%) in ND versus C pigs, regardless of in utero treatment. In summary, increasing the nutrient density of the diet did not rescue the lost productivity of IUHS pigs as indicated by an overall reduction in ADG.
40 Resilience of the ocular microbiome in beef calves
Authors: Bartenslager Alison , Aluthge Nirosh , Loy John , Spangler Matthew , Fernando Samodha
Published: November 2020

Infectious Bovine Keratoconjuctivitis (IBK), or more commonly known as pinkeye, impacts the beef cattle industry with expenses reaching close to 150 million dollars annually. Thus far, successful prevention of infectious outbreaks of IBK have been limited. Partly, this may be due to our limited understanding of the establishment and composition of the ocular microbiome. However, understanding of the establishment and composition of the ocular microbiome, may provide indicators species for early detection of IBK and to identify a window of opportunity to treat IBK before outbreaks occur. As an attempt to characterize the establishment and colonization patterns of the ocular microbiome in beef calves, we conducted a longitudinal study utilizing 239 calves. The calves were placed on three different preventative treatments which included an initial vaccination followed by a booster 3 weeks later (Autogenous, Commercial, and Placebo). Ocular swabs were collected at four different time points during summer and fall months. The V4 region of the 16S rDNA gene was sequenced from the 889 samples collected using the Illumina Miseq™ platform. The resulting sequences were analyzed using the Dada2 pipeline. The analysis demonstrated a significant difference in community composition (p< 0.001) across time periods sampled, where the microbiome reverted back to the original composition within three months of ocular microbiome perturbation and vaccination, demonstrating the resilience of the ocular microbiome to perturbation. This suggests the ocular microbiome is stable at 30 days of age. We also identified Mycoplasma sp. and Moraxella sp. which are candidate organisms known to predispose animals to IBK. Moraxella was prevalent during initial sampling, while Mycoplasma demonstrated greater abundance at all time points. This study demonstrates the diversity and the resilience of the ocular microbiome in calves and the potential to develop probiotic interventions to reduce IBK outbreaks.
221 Effect of varying growth rates on early and aged pork loin and chop quality
Authors: Schunke Emily D , Lowell Jessica E , Stahl Chad A , Harsh Bailey N , Dilger Anna C
Published: November 2020

The pork industry has observed an upward trend in ending live weights, resulting in heavier hot carcass weights (HCW). Heavier HCW positively affects loin tenderness; however, the mechanism of this effect is unclear. One possibility is increased growth rate, associated with greater HCW, resulting in more tender loins. The objective was to determine the effect of growth rate on early and aged pork quality. Pigs (N=634) were divided into three groups based on average daily gain (kg/d) from 12-26wk of age; slow (< 0.96kg/d, n=96), intermediate (0.96-1.16kg/d, n=452), and fast (≥ 1.17kg/d, n=86). The MIXED procedure of SAS was used to evaluate the main effects of growth rate, breed, sex, and their interactions on loin quality. Birth and weaning weight did not differ between growth rate (P≥0.15) but, overall ADG was increased (P<0.001) in fast growing pigs by 0.15 kg/d. Fast and intermediate growing pigs had darker loins (P=0.03) by 0.23 units. Intermediate growing pigs had firmer loins (P=0.04) by 0.07 units. Ventral a* increased as growth rate increased (P=0.04) indicating fast growing pigs had the reddest loins (9.77 vs. 9.26 vs. 8.99). Aged ventral marbling, ultimate pH, purge loss, cook loss, instrumental tenderness, and chop moisture and extractable lipid did not differ (P≥0.32) between growth rate groups. Duroc-sired pigs had increased tenth rib back fat amongst all growth rate groups, but the magnitude of difference was much greater in the slow and intermediate groups. Between all growth rate groups, slow growing Pietrain-sired Pigs had the smallest loineye areas (LEA). Carcass yield, bone-in carcass cutting yield, and boneless carcass cutting yield did not differ (P≥0.37) between growth rate groups. While fast growth rates improved aged ventral visual color, instrumental tenderness did not differ between growth rate groups.
129 Effect of phytase on growth performance and carcass classification in growing-finishing pigs
Authors: Silva Caio A , Dias Cleandro P , Callegari Marco A , Souza Kelly L , Passos Adsos , Silva Claudia C
Published: November 2020

The objective of this experiment was to evaluate the optimal dosage of phytase fed to growing-finishing pigs. One hundred and twenty barrows, 71 d old and initial body weight 25.16 ± 2.80kg, were distributed in 40 pens according to a randomized complete block design and assigned to 5 treatments: 1. Positive control: diet formulated to meet or exceed the nutrient requirement of pigs (PC); 2. Negative control: PC diets formulated with 0,11% lower Ca and 0,13% lower available P (NC); 3. NC + 1,500 FYT of phytase; 4. NC + 3,000 FYT of phytase; 5. NC +4,500 FYT of phytase. The corn-soybean meal-based diets were formulated to be iso-nutrient and isoenergetic, except for Ca and av. P. The 4 diets were formulated according to a growing I (71-94 days of age), growing II (95-115d), finishing I (116-143 d) and finishing II (144-156 d) phases. Carcass traits were measured and submitted to the European Carcass Classification (SEUROP). Performance and carcass data were submitted to ANOVA, and regression analysis. There was a quadratic effect on FCR (P< 0.05) in growing I phase,; better FCR (quadratic, P< 0.05) on finishing II to 1,500 and 3,000 FYT (5.56 and 0.35%, respectively); an increase of 5.43 and 1.52% DWG in finishing II (quadratic, P< 0.05) and a reduced 6.60% to 4,500FYT; an improvement the total DWG and final weight (quadratic, P< 0.05) in 6.19 and 4.52%, and 5.27 and 3.57%, for 1,500 and 3,000 FYT, respectively. Phytase supplementation did not improve Carcass weight (P >0.05). The animals fed with 4,500 FYT diet had more carcasses classified as E (between 55–60% lean meat-SEUROP) compared other groups. Doses between 1,500 and 3,000 FYT improve FCR, DWG and final LW of growing and finishing pigs.
132 A longer adaptation time increased xylanases efficacy in improving dry matter and fiber digestibility in the small intestine of growing pigs fed insoluble fiber
Authors: Petry Amy , Gould Stacie , Huntley Nichole , Bedford Mike R , Patience John
Published: November 2020

The objective of this experiment was to investigate xylanase efficacy and impact of adaptation time on nutrient utilization in the small intestine of growing pigs fed insoluble fiber. Twenty gilts (30.6 ± 0.2 kg BW; n=5 per treatment) were surgically fitted with t-cannulae in the medial jejunum (292 ± 12 cm distal to the pyloric sphincter) and in the terminal ileum, housed individually, and randomly assigned to one of four dietary treatments: a low-fiber control (LF; 7.3% NDF), a 30% corn bran high-fiber control (HF; 27.2% NDF), HF + 100 mg xylanase/kg (HF+XY; Econase XT 25P; AB Vista, Marlborough, UK), and HF + 50 mg arabinoxylan-oligosaccharide/kg (HF+AX; 3-7 degrees of polymerization). Gilts were limit fed for three 17 d periods (P1, P2, or P3). Each period included 5 d of adaptation, 2 d of fecal collections, 3 d of ileal collections, and 3 d of jejunal collections. Data were analyzed as repeated records using a linear mixed model (PROC Mixed, SAS 9.4) with surgery date as a random effect, and treatment, period, and their interaction as fixed effects. There tended to be a treatment by period interaction for the apparent jejunal digestibility (AJD) of DM driven by HF+XY improving digestibility over time when compared to HF. Xylanase improved AJD of DM by 21.9% (12.04% vs. 15.4%) in P2, and 40.5% (12.18% vs. 20.49%) in P3 (Treatment Period, P = 0.054). Xylanase supplementation improved AJD of NDF, compared to HF, in P2 by 36.3% (13.7% vs. 21.5%), and in P3 by 28.6% (17.7 vs 24.8%; Treatment Period, P = 0.031). Supplementing xylanase improved the apparent ileal digestibity (AID) of DM over HF in P1 by 4.4%, in P2 by 3.7% (73.91% vs. 76.2 %), and in P3 by 7.4% (75.04% vs. 80.84%; Treatment Period P=0.043). In conclusion xylanase efficacy improved with increased adaptation time.
77 The effect of naturally occurring levels of multiple mycotoxins on growth performance and carcass parameters of grow-finish pigs
Authors: Ruckman Leigh , Gould Stacie , Patience John
Published: November 2020

Mycotoxins may not be an issue every year, but the proper environmental conditions can cause a spike in contaminated grains and cause severe economic impact on pork producers. The objective of this study was to determine the effect of naturally occurring infections of deoxynivalenol, zearalenone and fumonisins (DZF) on growth performance and carcass parameters in grow/finish pigs. One hundred pigs (BW 34.0 ± 0.9 kg; L337 × Camborough, PIC, Hendersonville, TN) were randomly assigned to 1 of 2 dietary treatments with 10 split-sex pens/treatment. The control diet (CTL) contained low levels of DZF and the CTL+DFZ diet contained high levels of DZF. Diets were fed in 4 phases over the 126-d experiment period. The CTL diet contained 1.6, 1.6, 1.8 and 1.2 mg deoxynivalenol/kg and CTL+DZF contained 9.2, 6.9, 5.8 and 3.8 mg deoxynivalenol/kg in the 4 diet phases, respectively. The CTL contained 0.30, 0.32, 0.51 and 0.32 mg zearalenone/kg and 0.7, 0.8, 0.8 and 0.9 mg total fumonisins/kg; CTL+DFZ contained 0.59, 0.72, 0.86 and 0.57 mg zearalenone/kg and 1.0, 1.1, 1.2 and 0.9 mg total fumonisins/kg for phases one through four, respectively. Data were analyzed using PROC MIXED of SAS (9.4) with treatment, sex, and their interaction as fixed effects. Compared to CTL, feeding CTL+DFZ decreased final BW (130.3 vs 120.5 kg; P< 0.001), ADG (0.95 vs 0.79 kg/d; P< 0.001), ADFI (2.73 vs 2.49 kg/d; P=0.016), and G:F (0.35 vs 0.32; P=0.043). Feeding CTL+DFZ decreased HCW (92.3 vs 89.4 kg; P=0.024) and increased dressing percentage (70.9 vs 74.3%; P=0.009) and tended to reduce loin depth (7.0 vs 6.8 cm; P=0.057) compared to CTL. Diet did not affect backfat depth or lean percentage (P >0.10). In conclusion, diets naturally contaminated with multiple mycotoxins reduced growth performance and adversely affected carcass parameters; pigs did not adapt over time to the mycotoxins.
73 Effects of dietary neutral detergent fiber and standardized ileal digestible lysine levels on growth performance and carcass characteristics of growing-finishing pigs
Authors: Orlando Uislei , Lu Ning , Vier Carine , Cast Wayne , Zhou Xun , Young Malachy
Published: November 2020

Our objective was to determine the effects of feeding different neutral detergent fiber (NDF) and standardized ileal digestible (SID) lysine (Lys) levels on performance of growing-finishing pigs from 30- to 130-kg. A total of 2,016 barrows and gilts (PIC 280×Camborough, initial BW of 29.6±0.53 kg) were allotted to a randomized complete block design with a 2×2 factorial arrangement. Dietary treatments (Table 1) consisted with two NDF levels (13% or 20%) and two SID Lys curves (PIC2008 or PIC2016). There were 21 mixed-sex pigs per pen, and 24 pens per treatment. The experiment diets were fed in 3 phases. Wheat, wheat by-products, peas, and canola oil were used with NRC (2012) loading values to achieve treatments, and all diets were formulated to be iso-caloric (2,420 kcal NE/kg). Experimental data were analyzed using generalized linear mixed models with pen as the experimental unit. Increasing NDF level resulted in a reduction (P< 0.05) in average daily gain (ADG), average daily feed intake, hot carcass weight, carcass yield, carcass ADG, and backfat depth (Table 2), and tended to decrease percentage of removals and mortality (P=0.10). Pigs fed PIC2016 Lys curve had greater ADG and carcass ADG as compared to those fed PIC2008 Lys curve (P< 0.05). There were NDF×Lys interactions (P< 0.10) for ADG, gain to feed ratio (G:F), carcass ADG, and carcass G:F. These response criteria were improved for pigs fed PIC2016 Lys curve compared to those fed PIC2008 Lys curve when NDF level was 13%; while no evidence of differences was observed at 20% NDF. Results indicate that increasing NDF level from 13 to 20% deteriorate performance of 30- to 130- kg pigs; and the improved growth performance of pigs fed PIC2016 Lys curve as compared to those fed PIC2008 Lys curve was only observed when lower NDF level was presented.
157 The effect of different inclusion levels of corn starch and fine ground corn with different conditioning temperatures or die thickness on pellet quality
Authors: Evans Caitlin E , Jones Cassandra K , Paulk Chad B , Stark Charles R
Published: November 2020

The objective of this experiment was to determine the effect of different inclusion levels of corn starch and fine ground corn with different conditioning temperature or die thickness on pellet quality. Experiment 1, treatments were arranged in 3×2 factorial design of corn starch inclusion level (0, 5 and 10%) and die thickness (4mm×13mm and 4mm×22 mm). Experiment 2, treatments were arranged in 3×2 factorial design of fine ground corn inclusion level (0, 10 and 20) and conditioning temperature (80 and 85°C) with treatments pelleted using a 4mm×22mm die (5.6 L:D). In both experiments, treatments were pelleted using a model CL-5 CPM pellet mill (Crawfordsville, IN). The result of experiment 1 demonstrated that there was no interaction between corn starch inclusion level and die thickness on modified pellet durability index (PDI), (P=0.636). Increasing die thickness from 12.7 to 22.2 mm increased PDI from 43 to 70% (P< 0.001). There was a linear decrease (P< 0.001) in PDI as the corn starch inclusion level increased from 0 to 10% (64, 60, and 46%, respectively). The result of experiment 2 demonstrated that there was no interaction between fine ground corn inclusion level and conditioning temperature on PDI (P=0.541). The fine ground corn inclusion level did not impact PDI (P=0.298). Increasing conditioning temperature from 80 to 85°C increased PDI (P< 0.001) from 76 to 85%, respectively (P< 0.001). Based on the results, the use of pure corn starch was not an effective binding agent in the feed when the diet contains at least 60% ground corn. The ratio of small corn particles to large corn particles in the diet did not impact pellet quality when the diets were conditioned above 80°C for 35 sec and then pelleted with a 5.6 L:D die. Increasing die thickness and conditioning temperature improved pellet quality.