bluuebunny/crossref_metadata_2025 huggingface dataset:
Techno-economic evaluation of biodiesel production using by-product as raw material and hydrotalcite-hydroxyapatite as catalyst
Authors: Vilas Bôas Renata , Almeida Laura de A de , Mendes Marisa F
Published: March 2022

Biodiesel is one of the main sources of renewable energy that can be obtained from oils and fats by transesterification. However, biodiesel produced from vegetable oils as a raw material is expensive. Thus, an alternative and inexpensive raw material such as vegetable oil deodorizing distillate (VODD) can be used as a raw material for the production of biodiesel. In this study, technical-economic analyzes were carried out in the biodiesel production process using VODD as raw material and hydrotalcite-hydroxyapatite as heterogeneous catalyst. Four different scenarios were considered for the economic technical evaluation of the biodiesel production process: heterogeneous catalysis, homogeneous catalysis, reduced ethanol flow in the process input stream, and different proportions of the biodiesel:diesel mixture. Biodiesel production presented a positive internal rate of return (IRR), and in comparison, with the minimum attractiveness rate (MAR), it was economically viable based on the sales prices of the revenues practiced. In the sensitivity analyses, it was observed that the catalyst acquisition price was the most critical factor in the economic analysis of the simulated plant. Using different catalysts, a comparative study showed that the heterogeneous catalyst makes the process less expensive with the purification steps. Thus, it can be confirmed that the results obtained from this study can open paths for new developments in the biodiesel process in relation to the use of residual raw material and new materials to be used as catalysts.
Imunização, conhecimento e orientações: uma visão dos graduandos da área da saúde
Authors: Menezes João Daniel de Souza , Lalucce Giovana Lara Lopes , Mota Maria Eduarda Dóro , Melo Maria Eduarda Moura , Del Vecchio Ana Lídia Vieira , Castiglioni Lilian , Piatto Vânia Belintani 
Published: March 2022

A compreensão dos profissionais acerca da importância da vacinação é de extrema importância já que é dever do profissional de saúde orientar, sendo fundamental a boa compreensão do mesmo acerca da vacinação. Objetivo: Compreender a visão e conhecimento dos graduandos da área da saúde em relação ao uso dos imunobiológicos. Métodos: Trata-se de uma pesquisa descritiva, com análise quantitativa, realizada em uma faculdade de enfermagem, medicina e psicologia, do interior de São Paulo, com 103 alunos dos cursos de enfermagem e medicina, no período de outubro a novembro de 2021, através da plataforma Google Forms. Resultados: A maioria dos alunos cursavam o terceiro ano de graduação (50,5%), seguidos do quarto ano (21,4%). A respeito do conteúdo de vacinação ser contemplado na graduação, 72,8% (75) responderam positivamente, sendo este conteúdo abordado predominantemente na disciplina de Vigilância em Saúde (34,42%), tendo também destaque as disciplinas de Imunologia (19,67%) e Saúde da Criança e do Adolescente (19,67%). Além disso, a maioria dos alunos 58,3% (60) não participou de nenhum curso ou evento voltado ao tema. Na análise do desempenho dos alunos a maioria dos alunos apresentaram desempenho muito satisfatório (45,63%) ou satisfatório (52,42%), sendo apenas uma minoria insatisfatória (1,94%). Percebe-se que ao longo da graduação a porcentagem de acertos aumentou. Conclusão: Conclui-se com este estudo que os graduandos de enfermagem e medicina apresentaram conhecimento satisfatório acerca do assunto abordado e que este conhecimento é progressivo ao longo dos anos, porém, identificou-se também a carência da busca mais aprofundada pelo tema vacinação.
Pelatihan Dan Bimbingan Teknis Pembuatan Peta Tanah Di Sekolah Menengah Atas Di Kota Kendari
Authors: Mey Djafar , Karim Jufri , Rustam La Ode , Fitriani Fitriani
Published: March 2022

Knowledge about the land and the skills in making land maps provided is one of the efforts made to create appropriate resources. Efforts to increase knowledge and skills that can be done are introduction of soil descriptions and profiles along with training in making land maps. The purpose of this training and technical guidance activity is to provide students with an understanding of the soil at the high school level, knowing about soil resources in detail to students, knowing the procedures for making a land map using Geographic Information Systems (GIS) and knowing how to conduct soil investigations in the field. The problem solving framework of this activity is carried out in several stages, namely preparation, preparation of training materials, presentation of material by resource persons, discussions and questions and answers, practice of making land maps, and practice of making soil profiles by dividing student groups, evaluating the results of activities and preparing reports. This training was held in SMA Neg. 4 Kendari and SMA Neg. 6 Kendari. The result of this training and technical guidance is that the trainees have added knowledge and skills in making land descriptions and profiles, as well as making land maps using the geogarfi information system application. Keywords:. Training Students, Land, Geographic Information Systems
Peningkatan Kesadaran Pengelolaan Sampah Terpadu Berbasis Masyarakat Pesisir di Kelurahan Lapulu Kota Kendari Tahun 2019
Authors: Majid Ruslan , Zainuddin Asnia , Yasnani Yasnani , Nirmala Fifi , Tina Lymbran
Published: March 2022

Permasalahan persampahan di Kelurahan Lapulu bukan hanya disebabkan karena peningkatan jumlah penduduk saja, namun disebabkan pula dari rendahnya tingkat pelayanan prasarana dan sarana dasar lingkungan khususnya dalam bidang pelayanan persampahan yang tidak tuntas sehingga menimbulkan adanya timbulan-timbulan sampah yang tidak terangkut setiap harinya. Program pengabdian ini bertujuan untuk memberikan pemahaman dan pengetahuan kepada masyarakat tentang pentingnya meningkatkan kesadaran pengelolaan sampah. Kegiatan dilaksanakan dalam 2 bentuk, yang pertama bersifat non-fisik berupa sosialisasi pengelolaan sampah sejak dini pada anak Sekolah Dasar, penyuluhan tentang pengelolaan sampah tingkat Rumah Tangga, Pelatihan Pembuatan tempat sampah terpadu, dan pembagian leaflet kesadaran pengelolaan sampah pada komunitas Pasar Lapulu. Kedua berupa intervensi fisik yaitu pembuatan insenerator sederhana dan gerakan kesadaran pengelolaan sampah di masyarakat. Kegiatan ini telah terlaksana secara maksimal melalui pemberdayaan masyarakat dalam membentuk kesadaran masyarakat dalam pengelolaan sampah yang dimulai dengan cara pemilahan sampah, pemanfaatan limbah sampah dengan konsep 3R, menyediakan tempat pembuangan sampah yang ramah lingkungan dan rutinitas membersihkan sampah bersama- sama.
Pemberdayaan Masyarakat Tani Dalam Meningkatkan Pendapatan Rumah Tangga Desa Mekar Jaya Kecamatan Dangia Kabupaten Kolaka Timur
Authors: Nurwati Nurwati , Husin Husin
Published: March 2022

Pelatihan ini berjudul Pemberdayaan Masyarakat Tani  Dalam Meningkatkan Pendapatan Rumah Tangga Desa Mekar Jaya Kecamatan Dangia Kabupaten Kolaka Timur Sulawesi Tenggara. Tujuan pelatihan ini adalah memberikan pemahaman kepada masyarakat Desa Mekar Jaya Kecamatan Dangia Kabupaten Kolaka Timur agar memahami pentingnya pemberdayaan masyarakat tani dalam meningkatkan pendapatan rumah tangga menuju kemandirian ekonomi dan peningkatan pendapatan bagi masyarakat tani. Metode yang digunakan dalam pelatihan adalah metode ceramah (pengarahan) dan penjelasan secara serempak terhadap masyarakat. Metode diskusi serta tanya jawab juga dilakukan setelah selesai diberikan ceramah (pengrahan/penjelasan) mengenai pentingnya pemberdayaan masyarakat tani dalam meningkatkan pendapatan rumah tangga menuju kemandirian ekonomi dan peningkatan pendapatan bagi masyarakat tani desa mekar jaya kecamatan dangia kabupaten kolaka timur, Sulawesi Tenggara. Dari evaluasi dan hasil yang telah dilakukan pada aktivitas pelatihan terhadap 15 orang kepada karyawan dan masyarakat maka ditemukan peningkatan pemahaman akan pentingnya “Pemberdayaan Masyarakat Tani Dalam Meningkatkan Pendapatan Rumah Tangga Menuju Kemandirian Ekonomi dan Peningkatan Pendapatan Bagi Masyarakat Tani Desa Mekar Jaya Kecamatan Dangia Kabupaten Kolaka Timur, Sulawesi Tenggara” dengan tingkat keberhasilan sebesar 85%.
PELATIHAN LOGICAL BLOCK CODING UNTUK INTERVENSI KEGIATAN ANAK-ANAK SELAMA SOCIAL DISTANCING COVID19 BAGI SISWA JENJANG SD-SMP DI PROVINSI SULAWESI TENGGARA
Authors: Muhtar Norma Muhtar , Cahyono Edi , Ruslan Ruslan , Tenriawaru Andi , Laome Lilis
Published: March 2022

Untuk memutus rantai pandemi Covid-19, pemerintah telah mengambil kebijakan pembatasan sosial seperti menjaga jarak sosial (social distancing) menghindari kontak fisik (physical distancing), dan tetap tinggal di rumah (stay at home). Dampak di bidang pendidikan, kegiatan pembelajaran seluruhnya dilakukan dari rumah secara online, yang memaksa anak-anak menggunakan gadget. Orang tua yang mulai resah karena anak-anak memakai gadget sepanjang hari. Ada kekawatiran anak-anak menggunakan gadget untuk bermain game setelah waktu belajar. Di sisi lain,  anak-anak muncul rasa jenuh, stres, cemas, dan frustasi karena harus berdiam di rumah ditemani gadget untuk belajar dan bermain tanpa ada tantangan yang bisa dihasilkan dari bermain gadget.Coding mentransformasi perilaku anak dari pemain game menjadi pembuat game dan membiasakan anak berpikir problem solving. Salah satu jenis coding yang sesuai untuk anak-anak yaitu Logical Block Coding yang menggunakan balok-balok kode dengan bentuk dan warna yang berbeda-beda agar pengguna dapat membedakan fungsi dari masing-masing balok agar tercipta sebuah fungsi atau program.Pelatihan logical block coding merupakan kegiatan KKN-Tematik untuk menjawab keresahan orang tua dan kebosanan anak dengan cara mengintervensi kegiatan anak melalui aktifitas produktif, merangsang logika anak, dan membuat anak senang serta selalu terbangkitkan mood setiap harinya, sehingga siswa-siswa SD dan SMP di Sulawesi Tenggara selalu gembira dan terjaga imun tubuh selama masa pandemi covid19.Pelatihan dilaksanakan selama empat minggu oleh 24 mahasiswa di tempat domisili masing-masing mahasiswa pada  17 kabupaten/kota. Setiap mahasiswa wajib melatih satu orang siswa SD dan satu orang siswa SMP secara tatap muka dan dimonitoring secara berkala oleh dosen pembimbing secara online. Materi pelatihan merujuk pada panduan tutorial teks dan media video dari dosen pembimbing.Luaran kegiatan diperoleh 48 game komputer sederhana, respon positif dari seluruh orangtua yang mendukung adanya pelatihan coding, dan ekspresi siswa yang senang karena bisa membuat game. Sebagian besar siswa merespon untuk belajar lebih lanjut tentang coding.
Informasi Pencegahan Wabah Virus Corona (Covid-19) untuk Mengantisipasi Penyebaran di Lingkungan Masyarakat Tani Kabupaten Konawe Selatan
Authors: Indarsyih Yusna , Syukur La Ode , Syahbudin Syahbudin , Aswani Aswani
Published: March 2022

The existence of the coronavirus outbreak (Covid-19), has an impact on all sectors of human life. In a situation of uncertainty, one of the important supports for basic needs is food. Experience of various crises, the food system is a determining factor. Low stocks, soaring prices are a phenomenon that allows chaos during times of crisis. In the context of the food system, besides the availability of food, it is also important to pay attention to agricultural business actors. The impact of the spread of the coronavirus outbreak is not only in urban areas but also in rural areas, making agriculture a vulnerable sector. This is related to the aging profile of farmers, the lack of information received by farmers and the lack of health infrastructure in the village, and most of them rely on physical labor to earn income. The health of farmers and farm workers is an important factor in saving our food production in the future. Therefore, the solution to the objective of this integrated KKN-Thematic community service program is the active participation of student volunteers participating in the Covid-19 Thematic KKN and help prevent the spread of the coronavirus outbreak by providing socialization information about physical distancing, and compulsory use a mask, wash hands or use a hand sanitizer to ensure healthy farming communities are protected. Carrying out community service social activities, providing agricultural production facilities assistance, distributing masks, and distributing information pamphlets on the prevention of the coronavirus outbreak.Keywords: Information, Prevention, Covid-19, Farmers Society
Mengelola Perpustakaan Guna Menumbuhkan Minat Literassi Pada Anak Usia Dini Melalui Cerita Dongeng Anak
Authors: Woelandari PG Dewi Sri , Prasetyo S Ery Teguh , Wahyu Novita , Dharmanto Agus
Published: March 2022

Kegiatan pengabdian kepada masyarakat yang akan dilaksanakan berupa pendampingan dan penyuluhan dalam mengelola perpustakaan guna menumbuhkan minat literasi pada anak usia dini melalui cerita dongeng anak Di RT.005/ RW.001, Kel. Marga Mulya, Kec. Bekasi Utara Kota Bekasi. Khalayak sasaran dalam kegiatan Abdi Masyarakat ini adalah Para warga setempat. Penyampaian materi dengan narasumber dosen-dosen dari Fakultas Ekonomi-Manajemen dan Fakultas Ilmu Komunikasi. Dengan metode penyampaian presentasi dan penjelasan di hadapan khalayak serta didukung dengan pemberian modul materi dan tanya jawab yang akan digunaan saat masa pembelajaran, serta pembenahan fisik perpustakaan. Setelahnya pembelajaran maka para khalayak sasaran mengaplikasikan pembelajaran dan pelatihan langsung. Ketersediaan tenaga ahli dari para Dosen Fakultas Ekonomi dan Bisnis-Manajemen, dan para tenaga pembantu dari Mahasiswa, peserta, Ketua RT.005/RW.001 serta dana pendukung dari Universitas merupakan pendukung terlaksananya kegiatan Abdi Mayarakat ini. Manfaat yang didapatkan peserta dari kegiatan Abdi Masyarakat ini antara lain dapat memberikan pengetahuan baru mengenai perpustakaan dan dapat menambah wawasan mengenai sumber belajar.Kata Kunci : Mengelola dan Minat Literassi
Sosialisasi dan Penguatan Sistem Informasi dan Pengelolaan Administrasi Desa dalam Menghadapi COVID-19
Authors: Kadir Ishak , Yamin Muh , Handrawan Handrawan , Ma’ruf Annas , Aksara La Ode Muhammad Bahtiar
Published: March 2022

Desa Ameroro merupakan desa yang cukup besar di Kabupaten Konawe. Namun untuk penyampaian informasi desa kepada masyarakat belum maksimal, terlebih di tengah pandemi Covid-19 ini. Penguatan sistem informasi dan pengelolaan administrasi desa sangatlah penting. Selain mempermudah penyampaian informasi kepada masyarakat desa, hal ini juga dapat menarik perhatian masyarakat selain masyarakat Desa Ameroro. Dengan adanya penguatan sistem informasi, dapat memperkenalkan desa ke masyarakat luas dan secara tidak langsung menunjukkan potensi desa. Pengabdian ini bertujuan untuk memperkenalkan dan memberikan solusi untuk penguatan sistem informasi dari Desa Ameroro. Pengabdian ini dilakukan dengan tahap-tahap berikut : (1) metode pelaksanaan kegiatan; (2) memberi materi pelatihan penggunaan sistem; (3) rencana evaluasi penggunaan; (4) jadwal pelaksanaan kegiatan. Sistem yang ditawarkan adalah dalam bentuk website resmi Desa Ameroro. Website ini akan diisi dengan informasi mengenai profil desa dan kegiatan-kegiatan yang terlaksana di Desa Ameroro. Dalam pengabdian ini akan mengajarkan penggunaan dan pengelolaan dari website tersebut agar bisa dioperasikan dengan baik oleh aparat desa dan bermanfaat bagi Desa Ameroro.Kata kunci : covid-19, sistem informasi, websiteAbstract
Penerapan Edukasi Saintek di Desa Transmigrasi Lalowiu Kecamatan Konda Kabupaten Konawe Selatan Untuk Membangun Masyarakat Yang Sehat dan Mandiri
Authors: Cahyono Edi , Arman Arman , Sudayasa Putu , Budiman Herdi , Ransi Natalis
Published: March 2022

Sebagai suatu desa transmigrasi, Desa Lalowiu terletak diperbatasan antara Kota Kendari dengan Kabupaten Konawe Selatan Propinsi Sulawesi Tenggara. Kondisi lingkungan desa ini pada umumnya belum terlalu sehat yang dapat menimbulkan beberapa penyakit tropis termasuk COVID-19 ini. Untuk meningkatkan mutu kehidupan transmigran, pemerintah telah menempuh berbagai upaya antara lain peningkatan pendidikan dan pembinaan kesehatan. Selain itu juga melakukan pembinaan usaha ekonomi yang meliputi pembinaan usaha ekonomi berupa pembinaan usaha produksi, pengolahan hasil pertanian serta pembinaan pemasaran dan kelembagaan ekonomi desa. Berdasrkan potensi dan permasalahan tersebut mendorong Universitas Halu Oleo untuk melakukan kegiatan pengabdian kepada masyarakat yang terintegrasi KKN-Tematik di daerah tersebut. Tujuan umum dari kegiatan pengabdian terintegrasi KKN Tematik ini adalah menciptakan masyarakat yang mandiri,  sehat dan cerdas. Namun secara khusus kegiatan pengabdian ini bertujuan: 1) Memberikan wawasan kepada masyarakat akan pentingnya kesehatan keluarga untuk penanggulangan COVID-19 dan penyakit lainnya, 2) Memberikan pengetahuan pola hidup bersih dan sehat di lingkungan keluarga dan sekolah, 3) Memberikan beberapa penerapan sains dan teknologi di daerah pedesaan. 4) Meningkatnya ketahanan pangan keluarga dengan memanfaatkan lahan pekarangan dengan menanam sayuran dan pemeliharaan unggas dan ikan menggunakan teknik pembudidayaan yang praktis.
Produksi Ayam Broiler Organik Kaya Protein Untuk Meningkatkan Imunitas Tubuh Masa Pandemic Covid-19
Authors: Zulkarnain Deki , Aku Achmad Selamet , Pagala Muhammad Amrullah , Tasse Andi Murlina , Malesi La , Has Hamdan
Published: March 2022

The Thematic Real Work Lecture (KKN-T) Halu Oleo University in Matabubu Village, Poasia District, Kendari City, Southeast Sulawesi, has the aim of activities to increase body immunity during the Covid-19 pandemic and increase community motivation to manage and raise public awareness of the importance of using masks during the COVID-19 pandemic, especially for poultry farmers by providing community empowerment efforts in development in the integrated livestock sector, program activities carried out by Thematic KKN Students in the form of material delivery to breeders, counseling to breeders and training and community assistance in making fertilizer compost. However, the presence of the covid-19 virus limits Thematic KKN students to be more with the community with association activities.
PENINGKATAN KAPASITAS MASYARAKAT DALAM TEKNIK PANEN & PENANGANAN PASCAPANEN PROPOLIS LEBAH TANPA SENGAT DI DESA ABENGGI KONSEL
Authors: Pujirahayu Niken , Marwah Sitti , Rosmarlinasiah Rosmarlinasiah , Kasim Safril , Tuheteru Faisal Danu , Mando La Ode Agus Salim , Kabe Abigael , Rahmatiah T Eka
Published: March 2022

Abenggi Village, Landono District, Southeast Sulawesi is an agricultural area bordering a forest area with great potential for developing honey bee cultivation. Several farmer groups have cultivated stingless bees, such as Trigona sp. However, this effort is still not optimal because the only product that has been used and can be sold is honey, while other products such as bee bread and propolis are still wasted and unused. The purpose of this activity is raise awareness of farmer group members about the importance of proper harvesting and processing techniques for each bee product and improve community skills in harvesting and processing bee products, especially processing propolis into propolis blocks as a source of health and increasing their income. The method used is a participatory method with the formation of target groups, lectures/discussions, and technical guidance on harvesting and making propolis blocks. Monitoring and evaluating the success of activities using pre-test and post-test methods. The results of the activity: (1) 100% bee farmer group participation, (2) knowledge and skills of group members in harvesting techniques and post-harvest processing of propolis into propolis blocks increased (3) people became aware of propolis processing techniques that are more hygienic, can be stored and sold.
Bimbingan Teknis Pembuatan Produk-Produk Pendukung Pertanian Dengan Prinsip-Prinsip Teknologi Tepat Guna Di Amohalo Kota Kendari
Authors: Umar Muhammad Zakaria , Faslih Arman , Sisworo Raden Rinova , Abadi Musram , Saparun Moh. , Aminur Aminur
Published: March 2022

Mitra pengabdian ini adalah Amohalo di RT. 19/RW. 08, Kelurahan Baruga, Kecamatan Baruga, Kota Kendari. Permasalahan mitra sebagai berikut: (1) kawasan Amohalo akan menjadi kawasan agrowisata berbasis pertanian organik; (2) tim pengabdi ikut berpartisipasi dalam isu global terkait penerapan Teknologi Tepat Guna. Kegiatan pengabdian ini ditujukan untuk bersinergi dengan masyarakat Amohalo RT. 19/RW. 08, Kelurahan Baruga, Kecamatan Baruga, Kota Kendari dalam membuat produk-produk pendukung pertanian dengan prinsip-prinsip TTG. Metode pelaksanaan kegiatan ini dilakukan dengan cara penyuluhan dan praktik. Hasil dari kegiatan ini sebagai berikut: pertama, para mahasiswa melapor ke kantor kelurahan setempat, Ketua RT, dan tokoh masyarakat setempat; Kedua, para mahasiswa memberikan informasi tentang tujuan dan program-program kegiatan KKN Tematik; Ketiga, para mahasiswa mengumpulkan donasi bahan kerja bekas pakai dari warga; Keempat, mahasiswa membersihkan bahan donasi tersebut; Kelima, mahasiswa membuat produk-produk tersebut bersama warga masyarakat dan menyerahkan kepada masyarakat; Kegiatan pembuatan produk-produk ini dilaksanakan dengan prinsip prinsip TTG seperti bersahaja, ramah lingkungan, dan ekonomis.
Peningkatan Kompetensi Keahlian Bidang Instalasi dan Maintenance Jaringan Komputer bagi Pelajar SMK Lingkup Kota Kendari
Authors: Nur Alam Wa Ode Siti , Aksara La Ode Muhammad Bahtiar , Surimi La , Subardin Subardin
Published: March 2022

Sekolah Menengah Kejuruan (SMK) merupakan salah satu bentuk satuan pendidikan formal yang menyelenggarakan pendidikan kejuruan pada jenjang pendidikan menengah sebagai lanjutan dari SMP/MTs. Lulusan siswa/siswi SMK dipersiapkan untuk masuk lapangan kerja. Salah satu jurusan pada SMK adalah Teknologi Informasi dan Komunikasi dengan kompetensi keahlian Teknik Komputer dan Jaringan (TKJ). Pada proses pembelajaran pada kompetensi keahlian Teknik komputer dan jaringan salah satu bagiannya adalah praktek. Permasalahan yang terjadi pada proses pembelajaran praktek adalah kegiatan tersebut tidak dapat dilaksanakan disekolah karena adanya pandemi covid 19 sehingga berdampak pada rendahnya keterampilan bagi siswa/siswi SMK tersebut. Melihat permasalahan tersebut maka dalam kegiatan program kemitraan masyarakat ini bertujuan untuk meningkatkan kompetensi dan keahlian bidang instalasi dan maintenance jaringan. Pelatihan ini diharapkan dapat meningkatkan keterampilan bagi siswa/siswi SMK tentang instalasi dan maintenance jaringan komputer. Proses peningkatan kompetensi dan keahlian siswa/siswi SMK ini dilasanakan dalam tiga tahap yaitu tahapan pertama persiapan meliputi tentang pemilihan dosen-dosen sesuai bidang kompetensi dan koordinasi dengan mitra objek pengabdian. Tahapan kedua yaitu pelaksanaan meliputi tentang perkenalan, pemaparan materi dan praktek. Tahapan ketiga yaitu evaluasi meliputi tentang evaluasi keterampilan siswa/siswi dan pelayanan bagi siswa/siswi jika ada materi yang kurang dipahami. Hasil dari program kemitraan masyarakat ini siswa/siswi menyambut positif kegiatan ini karena dapat melakukan praktek secara langsung.
ANALISIS PENERAPAN SISTEM INFORMASI AKUNTANSI SEKTOR KEUANGAN PUBLIK DALAM PENYUSUNAN ANGGARAN BERBASIS KINERJA PADA BIRO UMUM SEKRETARIAT DAERAH PROVINSI SULAWESI TENGGARA
Authors: Manggabarani Andi Izmuwardana , Banga Wempy , Amir Muhammad
Published: March 2022

Penelitian ini bertujuan untuk menganalisis bentuk penerapan sistem informasi akuntansi sektor keuangan publik dalam penyusunan anggaran berbasis kinerja pada Biro Umum Sekretariat Daerah Provinsi Sulawesi Tenggara. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian kausalitas dengan pendekatan kaulitatif. Informan penelitian dalam penelitian terdiri dari 7 (tujuh) orang, dimana tehnik pemilihan informan menggunakan tehnik criterion sampling. Metode pengumpulan data dilakukan menggunakan angket dan observasi, sedangkan analisis data dilakukan menggunakan metode analisis deskriptif dengan pendekatan kualitatif. Hasil penelitian ini menunjukkan bahwa Biro Umum Sekretariat Daerah Provinsi Sulawesi Tenggara telah melaksanakan penerapan sistem informasi akuntansi sektor keuangan publik dalam penyusunan anggaran berbasis kinerja pada Biro Umum Sekretariat Daerah Provinsi Sulawesi Tenggara. hal tersebut ditunjukkan dari penggunaan sistem informasi berupa Sistem Informasi Manajemen Daerah (SIMDA) Melalui penggunaan SIMDA ini, maka pelaksanaan proses penyusunan anggaran berbasis kinerja mulai dari mekanisme perencanaan hingga pada penyusunan anggaran dapat berjalan dengan tepat dan transparan. Selain itu, basis penerapan SIMDA yang secara terkomputersisasi juga akan menghasilkan mekanisme proses penetapan anggaran yang terarah dan konsisten dengan seluruh dokumen-dokumen terkait seperti Renja, Renstra, RKPD, dan dokumen lainnya yang akan menjadi dasar acuan dalam perumusan strategi penetapan, perencanaan strategi anggaran, hingga pada pembuatan program kerja.
Microglia Polarization: A Novel Target of Exosome for Stroke Treatment
Authors: Wan Teng , Huang Yunling , Gao Xiaoyu , Wu Wanpeng , Guo Weiming
Published: March 2022

The vast majority of cells in the human body are capable of secreting exosomes. Exosomes have become an important vehicle for signaling between cells. Exosomes secreted by different cells have some of the structural and functional properties of that cell and thus have different regulatory functions. A large number of recent experimental studies have shown that exosomes from different sources have different regulatory effects on stroke, and the mechanisms still need to be elucidated. Microglia are core members of central intrinsic immune regulatory cells, which play an important regulatory role in the pathogenesis and progression of stroke. M1 microglia cause neuroinflammation and induce neurotoxic effects, while M2 microglia inhibit neuroinflammation and promote neurogenesis, thus exerting a series of neuroprotective effects. It was found that there is a close link between exosomes and microglia polarization, and that exosome inclusions such as microRNAs play a regulatory role in the M1/M2 polarization of microglia. This research reviews the role of exosomes in the regulation of microglia polarization and reveals their potential value in stroke treatment.
Social Modeling of Virtual Healthy Food Intake
Authors: Bulsing Patricia Johanna , Salmon Stefanie Johanna
Published: March 2022

People tend to adapt the amount of their food intake to that of others around them. This so-called social modeling of eating has been extensively studied over the past decades. The current study complements these experiments and aims to investigate social modeling of healthy food intake using a video paradigm in which a virtual model consumed either a small or a large portion of apple. In addition, it was tested whether modeling effects of a virtual female confederate were equally strong in male and female participants. Thirty participants (13 female, 17 male) completed the low norm condition in which a virtual model consumed 30 g of apple. Another 30 participants (17 female, 13 male) were allocated to the high norm condition in which a virtual model consumed 100 g of apple. Participants completed an irrelevant task, after which their own intake of apple was measured. Average intake in the low norm condition was 3 g, average intake in the high norm condition was 26 g (p < 0.001). In conclusion, participants adapted their intake to that of the virtual model. This effect was found irrespective of gender; female and male participants equally adapted their intake to that of a female virtual model. Stimulating food intake via a virtual model in people who have difficulty to meet dietary requirements or inhibiting food intake in people who tend to consume too much would be interesting new steps in the development of healthcare applications.
A Novel Solution for Distal Dilation of Chronic Dissection After Repair Involving Visceral Branches: The Road Block Strategy
Authors: Li Yiming , Li Zhenjiang , Feng Jiaxuan , Feng Rui , Zhou Jian , Jing Zaiping
Published: March 2022

Aim

Notwithstanding that unprecedented endovascular progress has been achieved in recent years, it remains unclear what is the best strategy to preserve the blood perfusion of abdominal visceral arteries and promote positive aortic remodeling in patients with distal dilatation of chronic aortic dissection in abdominal visceral part (CADAV) after aortic repair. The present study developed a Road Block Strategy (RBS) to solve this conundrum.

Methods and Results

This prospective single-center clinical study included patients suffering from symptomatic distal dilatation of CADAV after aortic repair treated with RBS from January 2015 to December 2019 and followed up regularly for at least 2 years. Stent grafts were implanted first to cover distal tears and expand the true lumen. Device embolization was performed to induce proximal and distal segmental false lumen thrombosis (FLT) apart from the level of the ostia of vital branches. Successful RBS was performed in 13 patients. Significant differences were found in maximum true lumen diameter (p < 0.05), blood flow area in false lumen (FL) (p < 0.001), and the ratio of blood lumen to FL area (p < 0.05) between the pre-procedure and the latest follow-up results. No aortic rupture, vital branches occlusion, thoracic and abdominal pain, or death occurred during hospitalization and follow-up.

Conclusions

Our findings suggest that RBS is feasible in treating distal dilatation of chronic aortic dissection after prior proximal repair, inducing false lumen thrombosis, preventing deterioration of aortic dissection, and maintaining the patency of abdominal visceral arteries.
Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets
Authors: Wang Hanjay , Pong Terrence , Obafemi Oluwatomisin O. , Lucian Haley J. , Aparicio-Valenzuela Joy , Tran Nicholas A. , Mullis Danielle M. , Elde Stefan , Tada Yuko , Baker Sam W. , Wang Caroline Y. , Cyr Kevin J. , Paulsen Michael J. , Zhu Yuanjia , Lee Anson M. , Woo Y. Joseph
Published: March 2022

Newborn mammals, including piglets, exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). The electrophysiologic properties of this naturally regenerated myocardium have not been examined. We hypothesized that epicardial conduction is preserved after P1 MI in piglets. Yorkshire-Landrace piglets underwent left anterior descending coronary artery ligation at age P1 (n = 6) or P7 (n = 7), After 7 weeks, cardiac magnetic resonance imaging was performed with late gadolinium enhancement for analysis of fibrosis. Epicardial conduction mapping was performed using custom 3D-printed high-resolution mapping arrays. Age- and weight-matched healthy pigs served as controls (n = 6). At the study endpoint, left ventricular (LV) ejection fraction was similar for controls and P1 pigs (46.4 ± 3.0% vs. 40.3 ± 4.9%, p = 0.132), but significantly depressed for P7 pigs (30.2 ± 6.6%, p < 0.001 vs. control). The percentage of LV myocardial volume consisting of fibrotic scar was 1.0 ± 0.4% in controls, 9.9 ± 4.4% in P1 pigs (p = 0.002 vs. control), and 17.3 ± 4.6% in P7 pigs (p < 0.001 vs. control, p = 0.007 vs. P1). Isochrone activation maps and apex activation time were similar between controls and P1 pigs (9.4 ± 1.6 vs. 7.8 ± 0.9 ms, p = 0.649), but significantly prolonged in P7 pigs (21.3 ± 5.1 ms, p < 0.001 vs. control, p < 0.001 vs. P1). Conduction velocity was similar between controls and P1 pigs (1.0 ± 0.2 vs. 1.1 ± 0.4 mm/ms, p = 0.852), but slower in P7 pigs (0.7 ± 0.2 mm/ms, p = 0.129 vs. control, p = 0.052 vs. P1). Overall, our data suggest that epicardial conduction dynamics are conserved in the setting of natural heart regeneration in piglets after P1 MI.
The Physiologic Mechanisms of Paced QRS Narrowing During Left Bundle Branch Pacing in Right Bundle Branch Block Patients
Authors: Zhu Kailun , Sun Yali , Lin Manxin , Deng Yingjian , Li Linlin , Li Guiyang , Liu Jianghai , Wan Xingcai , Chang Dong , Li Qiang
Published: March 2022

Left bundle branch pacing (LBBP) is a physiological pacing technique that captures the left bundle branch (LBB) directly, causing the left ventricle (LV) to be excited earlier than the right ventricle (RV), resulting in a “iatrogenic” right bundle branch block (RBBB) pacing pattern. Several studies have recently shown that permanent LBBP can completely or partially narrow the wide QRS duration of the intrinsic RBBB in most patients with bradycardia, although the mechanisms by which this occurs has not been thoroughly investigated. This article presents a review of the LBBP in patients with intrinsic RBBB mentioned in current case reports and clinical studies, discussing the technique, possible mechanisms, future clinical explorations, and the feasibility of eliminating the interventricular dyssynchronization accompanied with LBBP.
Key Factors for Improving Rigor and Reproducibility: Guidelines, Peer Reviews, and Journal Technical Reviews
Authors: Lu Hong S. , Daugherty Alan
Published: March 2022

To respond to the NIH's policy for rigor and reproducibility in preclinical research, many journals have implemented guidelines and checklists to guide authors in improving the rigor and reproducibility of their research. Transparency in developing detailed prospective experimental designs and providing raw data are essential premises of rigor and reproducibility. Standard peer reviews and journal-specific technical and statistical reviews are critical factors for enhancing rigor and reproducibility. This brief review also shares some experience from Arteriosclerosis, Thrombosis, and Vascular Biology, an American Heart Association journal, that has implemented several mechanisms to enhance rigor and reproducibility for preclinical research.
Heavy and Light Mineral Association of Late Quaternary Permafrost Deposits in Northeastern Siberia
Authors: Schirrmeister L. , Wetterich S. , Schwamborn G. , Matthes H. , Grosse G. , Klimova I. , Kunitsky V. V. , Siegert C.
Published: March 2022

We studied heavy and light mineral associations from two grain-size fractions (63–125 μm, 125–250 µm) from 18 permafrost sites in the northern Siberian Arctic in order to differentiate local versus regional source areas of permafrost aggradation on the late Quaternary time scale. The stratigraphic context of the studied profiles spans about 200 ka covering the Marine Isotope Stage (MIS) 7 to MIS 1. Heavy and light mineral grains are mostly angular, subangular or slightly rounded in the studied permafrost sediments. Only grains from sediments with significantly longer transport distances show higher degrees of rounding. Differences in the varying heavy and light mineral associations represent varying sediment sources, frost weathering processes, transport mechanisms, and post-sedimentary soil formation processes of the deposits of distinct cryostratigraphic units. We summarized the results of 1141 microscopic mineral analyses of 486 samples in mean values for the respective cryostratigraphic units. We compared the mineral associations of all 18 sites along the Laptev Sea coast, in the Lena Delta, and on the New Siberian Archipelago to each other and used analysis of variance and cluster analysis to characterize the differences and similarities among mineral associations. The mineral associations of distinct cryostratigraphic units within several studied profiles differ significantly, while others do not. Significant differences between sites as well as between single cryostratigraphic units at an individual site exist in mineral associations, heavy mineral contents, and mineral coefficients. Thus, each study site shows individual, location-specific mineral association. The mineral records originate from multiple locations covering a large spatial range and show that ratios of heavy and light mineral loads remained rather stable over time, including glacial and interglacial periods. This suggests mostly local sediment sources and highlights the importance of sediment reworking under periglacial regimes through time, including for example the formation of MIS 1 thermokarst and thermo-erosional deposits based on remobilized MIS 3 and 2 Yedoma Ice Complex deposits. Based on the diverse mineralogical results our study supports the viewpoint that Yedoma Ice Complex deposits are mainly results of local and polygenetic formations (including local aeolian relocation) superimposed by cryogenic weathering and varying climate conditions rather than exclusive long distance aeolian transport of loess, which would have highly homogenized the deposits across large regions.
Assessment of the Emerging Threat Posed by Perfluoroalkyl and Polyfluoroalkyl Substances to Male Reproduction in Humans
Authors: Calvert Leah , Green Mark P. , De Iuliis Geoffry N. , Dun Matthew D. , Turner Brett D. , Clarke Bradley O. , Eamens Andrew L. , Roman Shaun D. , Nixon Brett
Published: March 2022

Per-fluoroalkyl and polyfluoroalkyl substances (PFAS) are a diverse group of synthetic fluorinated chemicals used widely in industry and consumer products. Due to their extensive use and chemical stability, PFAS are ubiquitous environmental contaminants and as such, form an emerging risk factor for male reproductive health. The long half-lives of PFAS is of particular concern as the propensity to accumulate in biological systems prolong the time taken for excretion, taking years in many cases. Accordingly, there is mounting evidence supporting a negative association between PFAS exposure and an array of human health conditions. However, inconsistencies among epidemiological and experimental findings have hindered the ability to definitively link negative reproductive outcomes to specific PFAS exposure. This situation highlights the requirement for further investigation and the identification of reliable biological models that can inform health risks, allowing sensitive assessment of the spectrum of effects of PFAS exposure on humans. Here, we review the literature on the biological effects of PFAS exposure, with a specific focus on male reproduction, owing to its utility as a sentinel marker of general health. Indeed, male infertility has increasingly been shown to serve as an early indicator of a range of co-morbidities such as coronary, inflammatory, and metabolic diseases. It follows that adverse associations have been established between PFAS exposure and the incidence of testicular dysfunction, including pathologies such as testicular cancer and a reduction in semen quality. We also give consideration to the mechanisms that render the male reproductive tract vulnerable to PFAS mediated damage, and discuss novel remediation strategies to mitigate the negative impact of PFAS contamination and/or to ameliorate the PFAS load of exposed individuals.
Case Report: New CDKN1B Mutation in Multiple Endocrine Neoplasia Type 4 and Brief Literature Review on Clinical Management
Authors: Lavezzi Elisabetta , Brunetti Alessandro , Smiroldo Valeria , Nappo Gennaro , Pedicini Vittorio , Vitali Eleonora , Trivellin Giampaolo , Mazziotti Gherardo , Lania Andrea
Published: March 2022

Background

The fourth type of multiple endocrine neoplasia (MEN) is known as a rare variant of MEN presenting a MEN1-like phenotype and originating from a germline mutation in CDKN1B. However, due to the small number of cases documented in the literature, the peculiar clinical features of MEN4 are still largely unknown, and clear indications about the clinical management of these patients are currently lacking. In order to widen our knowledge on MEN4 and to better typify the clinical features of this syndrome, we present two more cases of subjects with MEN4, and through a review of the current literature, we provide some possible indications on these patients’ management.

Case Presentation

The first report is about a man who was diagnosed with a metastatic ileal G2-NET at the age of 34. Genetic analysis revealed the mutation p.I119T (c.356T>C) of exon 1 of CDKN1B, a mutation already reported in the literature in association with early-onset pituitary adenomas. The second report is about a 76-year-old woman with a multifocal pancreatic G1-NET. Genetic analysis identified the CDKN1B mutation c.482C>G (p.S161C), described here for the first time in association with MEN4 and currently classified as a variant of uncertain significance. Both patients underwent biochemical and imaging screening for MEN1-related diseases without any pathological findings.

Conclusions

According to the cases reported in the literature, hyperparathyroidism is the most common clinical feature of MEN4, followed by pituitary adenoma and neuroendocrine tumors. However, MEN4 appears to be a variant of MEN with milder clinical features and later onset. Therefore, these patients might need a different and personalized approach in clinical management and a peculiar screening and follow-up strategy.
Clinical Characteristics and Pathogenic Gene Identification in Chinese Patients With Paget’s Disease of Bone
Authors: Tao Xiaohui , Liu Li , Yang Xingguang , Wei Zhe , Chen Zhongzhong , Zhang Ge , Zhang Zhenlin , Yue Hua
Published: March 2022

Objective

To evaluate the clinical features of sporadic Paget’s disease of bone (PDB) in China and further explore the underlying genetic abnormalities of the disease.

Methods

Clinical characteristics, biochemical indices, bone turnover markers and radiographic examinations of the patients were collected. Genomic DNA was extracted from peripheral blood and whole-exome sequencing was carried out to identify the potential pathogenic genes. The pathogenicity of the variants was thereafter investigated by bioinformatics analysis.

Results

A total of 50 patients (57.20 ± 15.52 years, male/female: 1.63: 1) with PDB were included and the mean onset age was 48.34 years (48.34 ± 17.24 years). 94.0% of the patients exhibited symptomatic patterns described as bone pain (86.0%), elevated skin temperature at the lesion site (26.0%), bone deformity (22.0%) and local swelling (18.0%). The most frequently involved lesion sites were pelvis (52.0%), femur (42.0%), tibia (28.0%), skull (28.0%) and spine (18.0%), respectively. Additionally, 40.0% of them accompanied with osteoarthritis, 14.0% with pathological fractures, and the misdiagnosis rate of PDB was as high as 36.0%. Serum level of alkaline phosphatase was significantly increased, with the mean value of 284.00 U/L (quartiles, 177.00-595.00 U/L). Two heterozygous missense mutations of SQSTM1 gene (c.1211T>C, M404T) and one novel heterozygous missense mutation in HNRNPA2B1 gene (c.989C>T, p. P330L) were identified in our study. Moreover, several potential disease-causing genes were detected and markedly enriched in the pathways of neurodegeneration (including WNT16, RYR3 and RYR1 genes) and amyotrophic lateral sclerosis (ALS, including NUP205, CAPN2, and NUP214 genes).

Conclusion

In contrast to Western patients, Chinese patients have an earlier onset age, more severe symptoms, and lower frequency of SQSTM1 gene mutation (4.0%). Moreover, a novel heterozygous missense mutation in HNRNPA2B1 gene was identified in one male patient with isolated bone phenotype. As for other genetic factors, it was indicated that WNT16, RYR3, RYR1, NUP205, CAPN2 and NUP214 genes may be potential pathogenic genes, pathways of neurodegeneration and ALS may play a vital role in the pathogenesis of PDB.
miR-145-5p Inhibits Neuroendocrine Differentiation and Tumor Growth by Regulating the SOX11/MYCN Axis in Prostate cancer
Authors: Ji Shuya , Shi Yi , Yang Lin , Zhang Feng , Li Yong , Xu Feng
Published: March 2022

Recent studies have shown that the downregulation of miR-145-5p in prostate cancer (PCa) is significantly associated with poor differentiation and prognosis. We aimed to investigate the biological role of miR-145-5p in the neuroendocrine differentiation (NED) of PCa. In this study, TheCancer Genome Atlas was used to identify the association of miR-145-5p with PCa. The functions of miR-145-5p were evaluated using the Cell Counting Kit-8 (CCK-8) assay and cell cycle analysis. We validated changes in cell cycle control by testing the expression of cyclin-related genes by western blot. The luciferase reporter assay was performed to test miR-145-5p-targeting genes and direct transcriptional targets of SOX11. The expression of miR-145-5p was found to be significantly downregulated in castration-resistant PCa, and this was correlated with higher Gleason score and prostate-specific antigen. We confirmed these results using PC3 and LNCaP cell lines depicted a gradual decline of miR-145-5p while the cells were cultured under androgen depletion conditions. Moreover, the knockdown of miR-145-5p significantly promoted NED and proliferation of LNCaP cells, whereas overexpression of miR-145-5p significantly inhibited NED and proliferation of LNCaP cells. Mechanistically, we found that SOX11 was a direct target of miR-145-5p, which regulates MYCN might mediate induction of NED and proliferation of LNCaP cells. Furthermore, knockdown of miR-145-5p promoted tumor growth in vivo. Our findings suggest that miR-145-5p can inhibit NED and tumor growth by targeting SOX11, which regulates the expression of MYCN, and that this could be a novel therapeutic strategy for preventing the progression of PCa.
X Chromosome Inactivation Timing is Not eXACT: Implications for Autism Spectrum Disorders
Authors: LaSalle Janine M.
Published: March 2022

The etiology of autism spectrum disorders (ASD) is complex, involving different combinations of genetic and environmental factors. My lab’s approach has been to investigate DNA methylation as a tractable genome-wide modification at the interface of these complex interactions, reflecting past and future events in the molecular pathogenesis of ASD. Since X-linked genes were enriched in DNA methylation differences discovered from cord blood from newborns later diagnosed with ASD, this has prompted me to review and revisit the recent advancements in the field of X chromosome inactivation (XCI), particularly in humans and other primates. In this Perspective, I compare XCI mechanisms in different mammalian species, including the finding of the noncoding transcript XACT associated with X chromosome erosion in human pluripotent stem cells and recent findings from non-human primate post-implantation embryos. I focus on the experimentally challenging peri- and post-implantation stages of human development when the timing of XCI is prolonged and imprecise in humans. Collectively, this research has raised some important unanswered questions involving biased sex ratios in human births and the male bias in the incidence of ASD.
Antigen-Presenting B Cells Program the Efferent Lymph T Helper Cell Response
Authors: Alsén Samuel , Cervin Jakob , Deng Yaxiong , Szeponik Louis , Wenzel Ulf Alexander , Karlsson Joakim , Cucak Helena , Livingston Megan , Bryder David , Lu Qianjin , Johansson-Lindbom Bengt , Yrlid Ulf
Published: March 2022

B cells interact with T follicular helper (Tfh) cells in germinal centers (GCs) to generate high-affinity antibodies. Much less is known about how cognate T–B-cell interactions influence Th cells that enter circulation and peripheral tissues. Therefore, we generated mice lacking MHC-II expressing B cells and, by thoracic duct cannulation, analyzed Th cells in the efferent lymph at defined intervals post-immunization. Focusing on gut-draining mesenteric lymph nodes (MLNs), we show that antigen-specific α4β7+ gut-homing effector Th cells enter the circulation prior to CXCR5+PD-1+ Tfh-like cells. B cells appear to have no or limited impact on the early generation and egress of gut-homing Th cells but are critical for the subsequent appearance of Tfh-like cells that peak in the lymph before GCs have developed. At this stage, antigen-presenting B cells also reduce the proportion of α4β7+ Th cells in the MLN and efferent lymph. Furthermore, cognate B-cell interaction drives a broad transcriptional program in Th cells, including IL-4 that is confined to the Tfh cell lineage. The IL-4-producing Tfh-like cells originate from Bcl6+ precursors in the LNs and have gut-homing capacity. Hence, B cells program the efferent lymph Th cell response within a limited window of time after antigenic challenge.