bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental sensitivity analysis of the global properties of a two-dimensional turbulent wake
Authors: Parezanović Vladimir , Cadot Olivier
Published: January 2012

The sensitivity of the global properties of a two-dimensional turbulent wake produced by the separated flow of a ‘D’-shaped cylinder at$\mathit{Re}= 13\hspace{0.167em} 000$is investigated experimentally using a small circular control cylinder as a local disturbance. The height of the main cylinder is$D= 25~\mathrm{mm} $and control cylinders are of diameters$d= 0. 04D$and$d= 0. 12D$, the former being smaller than the shear layer thickness detaching from the main cylinder, while the latter is larger. In both cases, the control cylinder is able to modify the global frequency, base pressure and spanwise velocity correlation. The results are presented as sensitivity maps. Reynolds stresses spatial structure and the recirculation bubble length are examined in detail when the control cylinder is displaced vertically across the wake at a fixed downstream location. It is found that the increase of the recirculation bubble length is accompanied by a damping of Reynolds stresses with a downstream shift of their spatial structures together with the base pressure increase. The global frequency can be either decreased or increased independently of the bubble length modification. The sensitivity of these global properties is interpreted on the basis of the ability of the control cylinder to change the size of the formation region of the Kármán vortex street by interacting with the primary detached shear layers. The corresponding physical mechanisms are discussed. The impact of a two-dimensional control cylinder on the three-dimensional properties of the wake is examined through spanwise correlation. This is found to be improved whenever the control cylinder is placed inside the recirculation region of the main cylinder wake.
Near-wall streak modification by spanwise oscillatory wall motion and drag-reduction mechanisms
Authors: Touber Emile , Leschziner Michael A.
Published: January 2012

Direct numerical simulations for fully developed channel flow, subjected to oscillatory spanwise wall motion, have been performed and analysed in an effort to illuminate the fundamental mechanisms responsible for the reduction in turbulent friction drag, observed to result from the spanwise wall motion. A range of statistical data are discussed, including second-moment budgets, joint-probability-density functions, enstrophy and energy-spectra maps. Structural features are also investigated by reference to the response of streak properties to the oscillatory forcing. The unsteady cross-flow straining is shown to cause major spanwise distortions in the streak near-wall structures, leading to a pronounced reduction in the wall-normal momentum exchange in the viscous sublayer, hence disrupting the turbulence contribution to the wall shear stress. The response of the streaks, in terms of their periodic reorientation in wall-parallel planes, the decline and recovery of their intensity during the cyclic actuation, and their wall-normal coherence, is shown to be closely correlated with the temporal variation of the shear-strain vector. Furthermore, a modulating ‘top-to-bottom’ effect, associated with large-scale outer-layer structures, is highlighted and deemed responsible for the observed reduction in the actuation efficiency as the Reynolds number is increased.
The major soyabean allergen P34 resists proteolysisin vitroand is transported through intestinal epithelial cells by a caveolae-mediated mechanism
Authors: Sewekow Eva , Bimczok Diane , Kähne Thilo , Faber-Zuschratter Heidi , Kessler Lars Christian , Seidel-Morgenstern Andreas , Rothkötter Hermann-Josef
Published: January 2012

Soya is considered to be one of the eight most significant food allergens. Among the allergenic soya proteins determined to date, P34 has been identified as one of the immunodominant soya antigens. Sensitisation to a specific food antigen like P34 generally follows the transit of intact antigens across the intestinal barrier and usually occurs in infants, who are most susceptible to food allergies. In the present study, we used the intestinal epithelial cell line IPEC-J2, which was originally derived from the jejunum of a neonatal piglet, to recapitulate the infant intestinal epithelium and study the binding and uptake of P34 protein. P34 was partially resistant to degradation in anin vitroproteolysis assay. IPEC-J2 cells were able to endocytose intact P34, as shown by immunofluorescence and immunoelectronmicroscopy methods. P34 associated with lipid raft microdomains of IPEC-J2 cells, and disruption of caveolae/lipid raft microdomains using methyl-β-cyclodextrin abolished P34 endocytosis, indicating that the observed endocytosis was mediated by caveolae. Using IPEC-J2 cells grown on Transwell filters, we further demonstrated that P34 is transported through the epithelial monolayer by transcytosis. Piglets frequently show hypersensitivity to soya antigens, and in this study, we show that healthy adult pigs with dietary exposure to soya protein mount an antibody response to soyabean protein P34, suggesting that this protein has entered the body, probably through gastrointestinal uptake. In summary, our data suggest that soya P34 resists proteolysis in the gastrointestinal tract and is transported through the intestinal epithelial barrier, thereby allowing sensitisation of immune cells in the sub-epithelial compartment.
Dietary Approaches to Stop Hypertension (DASH) eating pattern and risk of elevated blood pressure in adolescent girls
Authors: Moore Lynn L. , Bradlee M. Loring , Singer Martha R. , Qureshi M. Mustafa , Buendia Justin R. , Daniels Stephen R.
Published: January 2012

Dietary determinants of adolescent blood pressure (BP) are poorly understood. The goal of the present study was to assess the effects of an eating pattern similar to that from the Dietary Approaches to Stop Hypertension (DASH) study on adolescent BP. Data from 2185 girls followed-up over 10 years (until the girls were 18–20 years of age) in the National Heart Lung and Blood Institute's Growth and Health Study were used in this analysis. Diet was assessed during eight examination cycles using 3 d dietary records; girls were classified according to their consumption of foods associated with a DASH-style eating pattern. Analysis of covariance modelling, multiple logistic regression and longitudinal mixed models were used to control for potential confounding by age, race, socio-economic status, height, physical activity, television viewing time and other dietary factors. Girls who consumed ≥ 2 daily servings of dairy and ≥ 3 servings of fruits and vegetables (FV) had a 36 % lower risk (95 % CI: 0·43, 0·97) of elevated BP (EBP) in late adolescence. In longitudinal modelling, two dietary factors were associated with a lower systolic BP throughout adolescence: higher ( ≥ 2 daily servings) dairy intakes (P < 0·0001) and a DASH-style pattern (P = 0·0002). Only the DASH-style pattern led to consistently lower diastolic BP levels (P = 0·0484). Adjustment for BMI did not appreciably modify the results. In this study, adolescent girls whose diets were rich in dairy products and FV during the early- and mid-adolescent years were less likely to have EBP levels in later adolescence.
An Early Ordovician tonalitic–granodioritic belt along the Schistose-Greywacke Domain of the Central Iberian Zone (Iberian Massif, Variscan Belt)
Authors: RUBIO-ORDÓÑEZ A. , VALVERDE-VAQUERO P. , CORRETGÉ L. G. , CUESTA-FERNÁNDEZ A. , GALLASTEGUI G. , FERNÁNDEZ-GONZÁLEZ M. , GERDES A.
Published: January 2012

The Zarza la Mayor and Zarza de Montánchez tonalites and Arroyo de la Luz granodiorite are part of a tonalitic–granodioritic belt located along the Schistose-Greywacke Domain of the Central Iberian Zone. These intrusions are also part of the Central Extremadura Batholith, a set of plutons ranging from tonalite to leucogranite that have been considered a prime example of Variscan syn-kinematic plutonism. New LA-ICP-MS and CA-ID-TIMS U–Pb dating reveals that the Zarza la Mayor tonalite–granodiorite is an Early Ordovician intrusion. The LA-ICP-MS data show that there is an absence of inherited cores, despite some complex internal zoning with obvious resorption features in some of the zircon crystals. Dating of monazite and zircon by CA-ID-TIMS provides a concordant age of 478.1 ± 0.8 Ma. This age coincides with electron microprobe analysis (EMPA) monazite chemical ages for the Zarza de Montánchez (482 ± 10 Ma) and Arroyo de la Luz (470 ± 15 Ma) intrusions. These new data indicate the presence of an Early Ordovician belt of calc-alkaline tonalite–granodiorite in the Schistose-Greywacke Domain – the Beira Baixa–Central Extremadura tonalite–granodiorite belt – which resembles a continental magmatic arc. This belt is contemporaneous with the Ollo de Sapo magmatic event further north in the Central Iberian Zone.
The Impact of Money on an African Subsistence Economy
Authors: Bohannan Paul
Published: January 2012

It has often been claimed that money was to be found in much of the African continent before the impact of the European world and the extension of trade made coinage general. When we examine these claims, however, they tend to evaporate or to emerge as tricks of definition. It is an astounding fact that economists have, for decades, been assigning three or four qualities to money when they discuss it with reference to our own society or to those of the medieval and modern world, yet the moment they have gone to ancient history or to the societies and economies studied by anthropologists they have sought the “real” nature of money by allowing only one of these defining characteristics to dominate their definitions.
‘The ethics of belief’ and belief about ethics: William Kingdon Clifford at the Metaphysical Society
Authors: CHRISTIAN ROSE ANN
Published: January 2012

As a member of the Victorian-era Metaphysical Society, W. K. Clifford contributed to debate about the prospects for morality in the absence of religion. Clifford thought its chances good. He presented a paper offering a ‘scientific’ approach to moral theory. In my discussion, I explore his proposal, using it to gain interpretative leverage on a paper he delivered before the Society only a year later, ‘The ethics of belief’. I set aside the quarrel with religion so prominent in this influential essay and discount its evidentialist epistemology, the better to reveal it for what it is: a powerful exercise in moral suasion.
A Thomistic metaphysics of creation
Authors: KERR GAVEN
Published: January 2012

This article seeks to advance a Thomistic metaphysics of creation in light of certain claims made by Stephen Hawking on the beginninglessness of the universe. I start with an exploration of Hawking's proposal that a beginningless universe entails an uncreated universe. This propels me into Aquinas's contention that a created beginningless universe is indeed possible, and thence I consider the metaphysics behind Thomas's position in this regard. Given this metaphysics of creation, I contend that there follow some interesting conclusions with regard to our notion of a creator God and the means for establishing the existence of such.
Rabbit haemorrhagic disease: are Australian rabbits (Oryctolagus cuniculus) evolving resistance to infection with Czech CAPM 351 RHDV?
Authors: ELSWORTH P. G. , KOVALISKI J. , COOKE B. D.
Published: January 2012

Rabbit haemorrhagic disease is a major tool for the management of introduced, wild rabbits in Australia. However, new evidence suggests that rabbits may be developing resistance to the disease. Rabbits sourced from wild populations in central and southeastern Australia, and domestic rabbits for comparison, were experimentally challenged with a low 60 ID50oral dose of commercially available Czech CAPM 351 virus – the original strain released in Australia. Levels of resistance to infection were generally higher than for unselected domestic rabbits and also differed (0–73% infection rates) between wild populations. Resistance was lower in populations from cooler, wetter regions and also low in arid regions with the highest resistance seen within zones of moderate rainfall. These findings suggest the external influences of non-pathogenic calicivirus in cooler, wetter areas and poor recruitment in arid populations may influence the development rate of resistance in Australia.
Evaluation of anopagene-based nucleic acid amplification test for detection ofNeisseria gonorrhoeaein urogenital samples in North India
Authors: VERMA R. , SOOD S. , BALA M. , MAHAJAN N. , KAPIL A. , SHARMA V. K. , PANDEY R. M. , SAMANTARAY J. C.
Published: January 2012

Due to the poor positive predictive value of nucleic acid amplification tests (NAATs) for gonorrhoea when applied to a low-prevalence setting, current guidelines recommend the use of supplementary polymerase chain reaction (PCR) targeting a different gene for confirmation of true positives in urogenital specimens. This study sought to standardize and evaluate performance of an in-houseopagene-based PCR assay for gonorrhoea compared to assays targeting theporApseudogene and 16S rRNA gene. Four hundred samples (300 endocervical, 100 urethral swabs) from patients attending STD clinics in New Delhi, India were used. The sensitivity, specificity, positive predictive value and negative predictive value of theopa-based PCR were 100%, 97·9%, 89·5% and 100%, respectively. In females, the use of NAATs provided enhanced diagnosis of gonorrhoea.
Non-traditional markers of metabolic risk in prepubertal children with different levels of cardiorespiratory fitness
Authors: Llorente-Cantarero Francisco Jesús , Pérez-Navero Juan Luis , de Dios Benitez-Sillero Juan , Muñoz-Villanueva Maria Carmen , Guillén-del Castillo Manuel , Gil-Campos Mercedes
Published: January 2012

Objective

To assess classical and non-classical metabolic risk biomarkers in prepubertal children with different levels of cardiorespiratory fitness (CRF).

Design

CRF was assessed by the 20 m shuttle run test. To estimate physical activity, participants were observed while engaged in an after-school programme. Additionally, a short test based on a validated questionnaire was used to obtain information about physical activity practice and sedentary habits. Anthropometric parameters, blood pressure, and classical and non-traditional metabolic risk biomarkers – plasma lipid profile, glucose and insulin, homeostasis model assessment–insulin resistance index (HOMA-IR), plasma uric acid, transaminases and C-reactive protein (CRP) – were measured.

Setting

The study was conducted in local elementary schools in Córdoba, Spain.

Subjects

One hundred and forty-one healthy children (eighty-eight boys, fifty-three girls) aged 7–12 years, in Tanner stage I, were recruited. They were divided into two groups after they performed the 20 m shuttle run test: equal or higher cardiovascular fitness (EHCF) group and low cardiovascular fitness (LCF) group.

Results

The LCF group displayed significantly higher TAG (P = 0·004) and lower HDL cholesterol levels (P = 0·001), as well as significantly lower values for the non-traditional lipid marker apo-A1 (P = 0·001) compared with the EHCF group. The LCF children displayed higher plasma glucose (P = 0·003) and insulin levels, higher HOMA-IR scores (P < 0·001) and higher plasma uric acid and CRP levels (P < 0·05). After adjustment for BMI, age and sex, no statistically significant differences were found between groups for the biomarkers analysed.

Conclusions

The study provides new information to understand the role not only of weight status but also of the level of CRF on the metabolic health profile of prepubertal children.
Parent Abuse by Young People on the Edge of Care: A Child Welfare Perspective
Authors: Biehal Nina
Published: January 2012

This article reports on parent abuse by 112 young people within a sample of 209 families with adolescent children who were receiving family support services, presenting both young people and parents’ accounts of this abuse. Drawing on an analysis of quantitative data, it also reports on the characteristics, histories and circumstances of the young people and families and examines the predictors of parent abuse. Lone mothers were more likely to be victims of this abuse. Many of the young people had experienced maltreatment and those who had witnessed domestic violence were more likely to be violent to parents.
A credibility method for profitable cross-selling of insurance products
Authors: Thuring Fredrik
Published: January 2012

A method is presented for identifying an expected profitable set of customers, to offer them an additional insurance product, by estimating a customer specific latent risk profile, for the additional product, by using the customer specific available data for an existing insurance product of the specific customer. For the purpose, a multivariate credibility estimator is considered and we investigate the effect of assuming that one (of two) insurance products is inactive (without available claims information) when estimating the latent risk profile. Instead, available customer specific claims information from the active existing insurance product is used to estimate the risk profile and thereafter assess whether or not to include a specific customer in an expected profitable set of customers. The method is tested using a large real data set from a Danish insurance company and it is shown that sets of customers, with up to 36% less claims than a priori expected, are produced as a result of the method. It is therefore argued that the proposed method could be considered, by an insurance company, when cross-selling insurance products to existing customers.
A comparison of three different pension savings products with special emphasis on the payout phase
Authors: Jørgensen Peter Løchte , Linnemann Per
Published: January 2012

The purpose of this article is to illustrate how the pension benefits a pension saver will (expect to) receive will depend on the type of pension scheme chosen. We compare three widely different pension savings products: the “traditional” with-profits scheme involving bonus entitlement (average interest rate product), a market-based Unit Link scheme and, finally, a formula based smoothed investment-linked annuity scheme – TimePension in short – which is on many points a cross between the two prior-mentioned types of savings products.

The three product types mentioned above have been analysed in previous literature, but those comparisons were based almost entirely on the values of pension savings accounts at the expiry of the accumulation period. This article will include the payout phase (decumulation phase) in the analysis, enabling us to analyse the size of paid-out pension benefits themselves as well as the possibilities of adjusting these benefits periodically. Compared to earlier articles, we have also improved the underlying model for the uncertainty of the underlying financial market.

The article demonstrates that expected pension benefits from the three schemes are an increasing function of the allocation to shares in the underlying investment portfolios. TimePension involves the highest allocation to shares and therefore offers, on average, the highest pension benefits, followed by the Unit Link scheme. In the third and last place comes the traditional with-profits scheme, which has a relatively low allocation to shares, but which, in return, also provides relatively safe and stable pension benefits. We also show, however, that the stability of pension benefits from a TimePension scheme is completely level with the stability of benefits from the traditional scheme. Unit Link-based pension benefits, on the other hand, vary far more, and pension savers in this product segment will experience much higher annual adjustments – in a both negative and positive direction – than savers in the other product segments.
Glycogen and its metabolism: some new developments and old themes
Authors: Roach Peter J. , Depaoli-Roach Anna A. , Hurley Thomas D. , Tagliabracci Vincent S.
Published: January 2012

Glycogen is a branched polymer of glucose that acts as a store of energy in times of nutritional sufficiency for utilization in times of need. Its metabolism has been the subject of extensive investigation and much is known about its regulation by hormones such as insulin, glucagon and adrenaline (epinephrine). There has been debate over the relative importance of allosteric compared with covalent control of the key biosynthetic enzyme, glycogen synthase, as well as the relative importance of glucose entry into cells compared with glycogen synthase regulation in determining glycogen accumulation. Significant new developments in eukaryotic glycogen metabolism over the last decade or so include: (i) three-dimensional structures of the biosynthetic enzymes glycogenin and glycogen synthase, with associated implications for mechanism and control; (ii) analyses of several genetically engineered mice with altered glycogen metabolism that shed light on the mechanism of control; (iii) greater appreciation of the spatial aspects of glycogen metabolism, including more focus on the lysosomal degradation of glycogen; and (iv) glycogen phosphorylation and advances in the study of Lafora disease, which is emerging as a glycogen storage disease.
Transcriptional regulation mechanisms of hypoxia-induced neuroglobin gene expression
Authors: Liu Ning , Yu Zhanyang , Xiang Shuanglin , Zhao Song , Tjärnlund-Wolf Anna , Xing Changhong , Zhang Jian , Wang Xiaoying
Published: January 2012

Ngb (neuroglobin) has been identified as a novel endogenous neuroprotectant. However, little is known about the regulatory mechanisms of Ngb expression, especially under conditions of hypoxia. In the present study, we located the core proximal promoter of the mouse Ngb gene to a 554 bp segment, which harbours putative conserved NF-κB (nuclear factor κB)- and Egr1 (early growth-response factor 1) -binding sites. Overexpression and knockdown of transcription factors p65, p50, Egr1 or Sp1 (specificity protein 1) increased and decreased Ngb expression respectively. Experimental assessments with transfections of mutational Ngb gene promoter constructs, as well as EMSA (electrophoretic mobility-shift assay) and ChIP (chromatin immunoprecipitation) assays, demonstrated that NF-κB family members (p65, p50 and cRel), Egr1 and Sp1 bound in vitro and in vivo to the proximal promoter region of the Ngb gene. Moreover, a κB3 site was found as a pivotal cis-element responsible for hypoxia-induced Ngb promoter activity. NF-κB (p65) and Sp1 were also responsible for hypoxia-induced up-regulation of Ngb expression. Although there are no conserved HREs (hypoxia-response elements) in the promoter of the mouse Ngb gene, the results of the present study suggest that HIF-1α (hypoxia-inducible factor-1α) is also involved in hypoxia-induced Ngb up-regulation. In conclusion, we have identified that NF-κB, Egr1 and Sp1 played important roles in the regulation of basal Ngb expression via specific interactions with the mouse Ngb promoter. NF-κB, Sp1 and HIF-1α contributed to the up-regulation of mouse Ngb gene expression under hypoxic conditions.
Basic Testing of the duchamp Source Finder
Authors: Westmeier T. , Popping A. , Serra P.
Published: January 2012

This paper presents and discusses the results of basic source finding tests in three dimensions (using spectroscopic data cubes) with duchamp, the standard source finder for the Australian Square Kilometre Array Pathfinder. For this purpose, we generated different sets of unresolved and extended Hi model sources. These models were then fed into duchamp, using a range of different parameters and methods provided by the software. The main aim of the tests was to study the performance of duchamp on sources with different parameters and morphologies and assess the accuracy of duchamp's source parametrisation. Overall, we find duchamp to be a powerful source finder capable of reliably detecting sources down to low signal-to-noise ratios and accurately measuring their position and velocity. In the presence of noise in the data, duchamp's measurements of basic source parameters, such as spectral line width and integrated flux, are affected by systematic errors. These errors are a consequence of the effect of noise on the specific algorithms used by duchamp for measuring source parameters in combination with the fact that the software only takes into account pixels above a given flux threshold and hence misses part of the flux. In scientific applications of duchamp these systematic errors would have to be corrected for. Alternatively, duchamp could be used as a source finder only, and source parametrisation could be done in a second step using more sophisticated parametrisation algorithms.
Local identities, identification and incorporation of Albanian immigrants in Florence
Authors: Vathi Zana
Published: January 2012

In this paper, the concepts of city as context, locality and incorporation are applied to the case of Albanian immigrants in Florence. To date, most research on the incorporation of immigrants and its relationship to space is based on the city or the inter-relations between the nation-state and city as contexts where incorporation takes place. The findings of this study show that the relationship between locality and the incorporation of immigrants is multi-dimensional. First, although findings support the role of the urban ethos in immigrants' incorporation, the city is not a homogeneous entity and should not be equated with locality where immigrants' incorporation is concerned. The importance of locality within the city is highlighted by the role played by the qualities of space and aesthetics in immigrants' incorporation and identification. Also, more attention should be paid to intergenerational perceptions of space, as the data show that different generations have different ‘cognitive geographies’ and varying perceptions of space and appreciations of cosmopolitanism, seen here in relation to locality. These aspects should be seen in relation to the historical, structural and cultural particularities of the city as a key context of incorporation, without ignoring the national-level politics of immigration and integration.
Reflections on the Croce—Dewey exchange
Authors: Vittorio Massimo
Published: January 2012

The well-known dispute between Benedetto Croce and John Dewey had several elements at its origin. This article provides a systematic overview of the issues that impinged on this affair, and of the many studies which have dwelt on the exchange between these two philosophers. The underlying American anti-Hegelianism, the language problem and the actual differences in thinking on aesthetics are all fundamental to this mutual misunderstanding. The reasons why Croce had made a limited impact in the United States are discussed. The analysis concludes by highlighting the socio-ethical aspects of Deweyan aesthetics.
Toward a natural classification: phylogeny of acontiate sea anemones (Cnidaria, Anthozoa, Actiniaria)
Authors: Rodríguez Estefanía , Barbeitos Marcos , Daly Marymegan , Gusmão Luciana C. , Häussermann Verena
Published: January 2012

Acontia—nematocyst‐dense, thread‐like extensions of the mesenterial filaments―are the characteristic feature of the actiniarian group Acontiaria. Phylogenetic analyses have shown that acontiate taxa form a clade that also includes some taxa without acontia. We analyse five molecular markers from 85 actiniarians to explore the phylogenetic relationships among families in Acontiaria, including acontiate species assigned to other higher taxa and species without acontia that have been allied to Acontiaria. Based on our results, we redefine the group to accommodate those lineages that have lost acontia, and formalize it as superfamily Metridioidea. Based on stable and well supported clades, we resurrect Phelliidae and Amphianthidae, redefine Kadosactinidae and Actinoscyphiidae, and move two species to new genera: that previously termed Sagartiogeton erythraios belongs in Jasonactis gen. nov.; and that previously termed Anthosactis pearseae belongs in Ostiactis gen. nov., type genus of Ostiactinidae fam. nov. We also synonymized Halcampoididae and Halcampidae (as Halcampidae) and Andvakiidae and Isophelliidae (as Andvakiidae). The results of our phylogenetic analyses indicate that the diagnostic morphological characters used in the family‐level taxonomy of acontiate actiniarians such as the nematocysts of the acontia, the marginal sphincter muscle, and mesenteries divisible into macro‐ and micro‐cnemes, have to be revisited, as these features are highly homoplasious.
The rising prevalence of prescription opioid injection and its association with hepatitis C incidence among street‐drug users
Authors: Bruneau Julie , Roy Élise , Arruda Nelson , Zang Geng , Jutras‐Aswad Didier
Published: January 2012

Aims  To examine trends in prescription opioid (PO) injection and to assess its association with hepatitis C virus (HCV) seroconversion among injection drug users (IDUs).

Design  Prospective cohort study.

Setting  Montreal, Canada.

Participants  HCV‐negative IDUs at baseline, reporting injection in the past month.

Measurements  Semi‐annual visits included HCV antibody testing and an interview‐administered questionnaire assessing risk behaviours. HCV incidence rate was calculated using the person–time method. Time‐updated Cox regression models were conducted to examine predictors of HCV incidence.

Findings  The proportion of IDUs reporting PO injection increased from 21% to 75% between 2004 and 2009 (P < 0.001). Of the 246 participants (81.6% male; mean age 34.5 years; mean follow‐up time 23 months), 83 seroconverted to HCV [incidence rate: 17.9 per 100 person‐years; 95% confidence interval (CI) 14.3, 22.1]. Compared to non‐PO injectors, PO injectors were more likely to become infected [adjusted hazard ratio (AHR): 1.87; 95%CI:1.16, 3.03]. An effect modification was also found: PO injectors who did not inject heroin were more likely to become infected (AHR: 2.88; 95%CI: 1.52, 5.45) whereas no association was found for participants using both drugs (AHR: 1.19; 95% CI: 0.61, 2.30). Other independent predictors of HCV incidence were: cocaine injection, recent incarceration and >30 injections per month.

Conclusions  Prescription opioid injectors who do not inject heroin are at greater risk for HCV seroconversion than are those injecting both heroin and prescription opioids. Important differences in age, behaviour and social context suggest a need for targeted outreach strategies to this population.
Prognostic importance of tumour-infiltrating memory T cells in oesophageal squamous cell carcinoma
Authors: Enomoto K , Sho M , Wakatsuki K , Takayama T , Matsumoto S , Nakamura S , Akahori T , Tanaka T , Migita K , Ito M , Nakajima Y
Published: January 2012

Memory T cells survive for many months and years and are critically important for host defence in humans. In tumour immunity, they have been also suggested to play a significant role in tumour progression and metastasis. However, the role of memory T cells in actual human cancer remains largely unknown. In this study, the clinical importance of tumour-infiltrating CD45RO+ memory T cells was investigated in human oesophageal squamous cell carcinoma (OSCC). CD45RO+ T cells were evaluated by immunohistochemistry in primary OSCC tumours from 105 patients. Patients were classified into two groups as CD45RO+hi or CD45RO+lo based on the number of cells stained positively for CD45RO. No significant difference was observed between CD45RO status and several clinicopathological prognostic factors. However, the postoperative overall and disease-free survival for CD45RO+hi patients was significantly better than for CD45RO+lo patients. Furthermore, there were significant correlations of CD45RO status in the primary tumour with postoperative lymph node and pulmonary recurrence, suggesting that memory T cells may control postoperative metastatic recurrence. Most importantly, CD45RO+ memory T cell status has a significant prognostic value for OSCC independently of conventional tumour–node–metastasis (TNM) classification. Our study may provide a rationale for developing a novel immunotherapy in intentional induction of memory T cells for the treatment of oesophageal cancer.
Type 1 and type 2 cervical carcinomas: some cervical cancers are more difficult to prevent with screening
Authors: Austin R. M. , Zhao C.
Published: January 2012

R. M. Austin and C. Zhao  Type 1 and type 2 cervical carcinomas: some cervical cancers are more difficult to prevent with screening

Although the Papanicolaou (Pap) smear is medical history’s most successful cancer screening test, some cervical cancers are more difficult to prevent with screening than others. Cervical cancers that are difficult to prevent are seen disproportionately among interval cancers arising in previously screened women and in Pap test litigation. These include (i) rapidly progressing cervical cancers; (ii) cervical cancers in younger women; (iii) glandular cervical cancers; and (iv) cervical cancers in elderly women. Screening protocols have generally been designed to optimize prevention of slower‐growing cervical squamous carcinomas in middle‐aged women. To focus further attention on the heterogeneous screening challenges posed by different cervical cancers, we designate the more screening preventable majority as type 1 cervical cancers and the more difficult to prevent minority as type 2 cervical cancers. We review available data on why some cervical cancers are more difficult to prevent with screening and newer methods that may improve prevention.
Spatial and temporal aquatic–terrestrial transitions in the temporary Albarine River, France: responses of invertebrates to experimental rewetting
Authors: DATRY THIBAULT , CORTI ROLAND , PHILIPPE MICHEL
Published: January 2012

1. In temporary rivers, viewed as coupled terrestrial–aquatic ecosystems, spatial and temporal transition zones between aquatic and terrestrial conditions are common and occur simultaneously.

2. The effects of artificial rewetting on terrestrial and aquatic invertebrate assemblages were examined in dry sediments collected from the Albarine River, France. Rewetted sediments had previously been dry for between 0.1 and 142 days. Dry sediments were collected directly from the streambed (DS) and from riparian gravel bars (RGB).

3. We first predicted that invertebrate responses to rewetting would vary with the duration of the preceding dry period. Second, we predicted convergence of the invertebrate assemblages in DS and RGB sediments with increasing duration of the dry period. Third, we predicted that an aquatic ‘invertebrate seedbank’ (aquatic life stages that persist within streambed sediments during dry periods) would contribute substantially to the resilience of benthic assemblages.

4. Results indicated that the duration of the dry period was the primary driver of aquatic and terrestrial responses to artificial rewetting. The density and richness of aquatic taxa decreased with the duration of the dry period in both DS and RGB sediments, whereas the density of terrestrial invertebrates increased in DS sediments.

5. No convergence between DS and RGB assemblage composition was observed with an increasing dry period. Although there were more aquatic organisms in DS sediments than in RGB sediments, there was no difference in taxonomic richness between sediment types. Even after prolonged dry periods (142 days), there was typically a lower density and taxonomic richness of terrestrial invertebrates in DS sediments than in adjacent RGB sediments.

6. The results suggest that the aquatic invertebrate seedbank could contribute substantially to the resilience of benthic assemblages in the Albarine River, in addition to other mechanisms such as drift and oviposition. Of the taxa in the benthos before and after the summer dry period, 65% were also recovered from artificially rewetted DS sediments. The simultaneous presence of temporal and spatial terrestrial–aquatic transition zones in temporary rivers increases successional diversity (i.e. mosaics of dry and saturated streambed patches at various stages of terrestrial and aquatic succession). This contribution to biodiversity emphasises the need to protect dry reaches of temporary rivers.
Carbohydrate‐active enzymes revealed in Coptotermes formosanus (Isoptera: Rhinotermitidae) transcriptome
Authors: Zhang D. , Lax A. R. , Henrissat B. , Coutinho P. , Katiya N. , Nierman W. C. , Fedorova N.
Published: January 2012

Coptotermes formosanus is one of the most destructive wood‐feeding termites. To understand the molecular mechanisms that regulate the development of the termite, a normalized C. formosanus cDNA library was constructed using mixed RNA isolated from workers, soldiers, nymphs and alates of both sexes. The sequencing of this library generated 131 636 expressed sequence tags (ESTs) and 25 939 assembled unigenes. The carbohydrate‐active enzymes (CAZymes) revealed in this library were analysed in the present report. A total of 509 putative CAZymes were identified. Diverse cellulolytic enzymes were uncovered from both the host termite and from symbionts harboured by the termite, which were possibly the result of the high efficiency of cellulose utilization. CAZymes associated with trehalose biosynthetic and metabolic pathways were also identified, which are potential regulators of the physiological activities of trehalose, an important insect blood sugar. Representative CAZyme coding genes in glycoside hydrolase family 1 (GH1) were quantitatively analysed. The results showed that the five GH1 β‐glucosidase genes were expressed differentially among different castes and one of them was female alate‐specific. Overall, the normalized EST library provides a comprehensive genetic resource of C. formosanus and will serve a diverse range of research areas. The CAZymes represent one of the repositories of enzymes useful for physiological studies and applications in sugar‐based biofuel production.
Changes in centring and shaping ability using three nickel–titanium instrumentation techniques analysed by micro‐computed tomography (μCT)
Authors: Stern S. , Patel S. , Foschi F. , Sherriff M. , Mannocci F.
Published: January 2012

Stern S, Patel S, Foschi F, Sherriff M, Mannocci F. Changes in centring and shaping ability using three nickel–titanium instrumentation techniques analysed by micro‐computed tomography (μCT). International Endodontic Journal, 45, 514–523, 2012.

Aim  To compare the centring ability and the shaping ability of ProTaper (PT) files used in reciprocating motion and PT and Twisted Files (TF) used in continuous rotary motion, and to compare the volume changes obtained with the different instrumentation techniques using micro‐computed tomography.

Methodology  Sixty mesial canals of thirty mandibular molars were randomly assigned to three instrumentation techniques: group 1, canals prepared with the PT series (up to F2) (n = 20); group 2, canals prepared with the F2 PT in reciprocating motion (n = 20); group 3 canals prepared with the TF series (size 25) (n = 20). Teeth were scanned pre‐ and post‐operatively using micro‐computed tomography to measure volume and shaping changes, and the obtained results were statistically analysed using parametric tests.

Results  The increase in canal volume obtained with the three instrumentation techniques was not significantly different. Canals were transported mostly towards the mesial aspect in the apical‐ and mid‐third of the roots, and towards the furcal aspect coronally. No difference in the transportation and centring ratio was found between the techniques. There was no significant difference between the times of instrumentation (TF: 62.5 ± 5.4 s; PT: 60.6 ± 3.9 s; and F2 PT file in reciprocating motion: 51.0 ± 3.3 s).

Conclusions  ProTaper files used in reciprocating motion and PT and TF used in continuous rotary motion were capable of producing centred preparations with no substantial procedural errors.
Evaluation of the propylene glycol association on some physical and chemical properties of mineral trioxide aggregate
Authors: Duarte M. A. H. , Alves de Aguiar K. , Zeferino M. A. , Vivan R. R. , Ordinola‐Zapata R. , Tanomaru‐Filho M. , Weckwerth P. H. , Kuga M. C.
Published: January 2012

Duarte MAH, Alves de Aguiar K, Zeferino MA, Vivan RR, Ordinola‐Zapata R, Tanomaru‐Filho M, Weckwerth PH, Kuga MC. Evaluation of the propylene glycol association on some physical and chemical properties of mineral trioxide aggregate. International Endodontic Journal, 45, 565–570, 2012.

Aim  To evaluate the influence of propylene glycol (PG) on the flowability, setting time, pH and calcium ion release of mineral trioxide aggregate (MTA).

Methodology  Mineral trioxide aggregate was mixed with different proportions of PG, as follows: group 1: MTA + 100% distilled water (DW); group 2: MTA + 80% DW and 20% PG; group 3: MTA + 50% DW and 50% PG; group 4: MTA + 20% DW and 80% PG; group 5: MTA + 100% PG. The ANSI/ADA No. 57 was followed for evaluating the flowability and the setting time was measured by using ASTM C266‐08. For pH and calcium release analyses, 50 acrylic teeth with root‐end cavities were filled with the materials (n = 10) and individually immersed in flasks containing 10 mL deionized water. After 3 h, 24 h, 72 h and 168 h, teeth were placed in new flasks and the water in which each specimen was immersed had its pH determined by a pH metre and the calcium release measured by an atomic absorption spectrophotometer with a calcium‐specific hollow cathode lamp. Data were analysed by using one‐way anova test for global comparison and by using Tukey’s test for individual comparisons.

Results  The highest value of flowability was observed with MTA + 20% DW and 80% PG and the lowest values were found with MTA + 100% DW. They were significantly different compared to the other groups (P < 0.05). The presence of PG did not affect the pH and calcium release. The MTA + 100% PG favoured the highest (P < 0.05) pH and calcium release after 3 h. Increasing the PG proportion interfered (P < 0.05) with the setting time; when used at the volume of 100% setting did not occur.

Conclusion  The addition of PG to MTA‐Angelus increased its setting time, improved flowability and increased the pH and calcium ion release during the initial post‐mixing periods. The ratio of 80% DW – 20% PG is recommended.
Presence of Not5 and ubiquitinated Rps7A in polysome fractions depends upon the Not4 E3 ligase
Authors: Panasenko Olesya O. , Collart Martine A.
Published: January 2012

In this study, we determine that Saccharomyces cerevisiae Not4 E3 ligase ubiquitinates Rps7A in vivo and in vitro, but not its paralogue, Rps7B. Ubiquitinated Rps7A is detectable only in 80S and polysomes, but not in free 40S fractions. A different role of the Rps7 paralogues in vivo is supported by the observation that the deletion of Rps7A but not Rps7B is sensitive to translational inhibitors and leads to an accumulation of aggregated proteins. An important accumulation of aggregated proteins that include ribosomal proteins and ribosome‐associated chaperones is also observed in cells lacking Not4. A contribution of Not4 to ribosomal function extending beyond Rps7A ubiquitination is supported by the observation that the deletion of Not4 displays a synthetic slow growth phenotype when combined with the deletion of either one of the two Rps7 paralogues. Not4 is detectable in polysome fractions, as are other subunits of the Ccr4–Not complex such as Not5. The optimal presence of Not5 in polysomes is dependent upon Not4 and the deletion of Not5 leads to a dramatic reduction of polysomes. These results lead us to suggest that Not4 contributes to normal polysome levels and is important for cellular protein solubility maybe in part by ubiquitination of Rps7A.
Reproduction and probing behaviour of the bird cherry‐oat aphid Rhopalosiphum padi on detached leaves and leaves on intact seedlings of the winter host, bird cherry Prunus padus
Authors: NAM KI JUNG , HARDIE JIM
Published: January 2012

Video recording and electrical penetration graph techniques are used simultaneously to investigate host acceptance (in terms of reproduction) and probing activities of autumn migrants (gynoparae) of the bird cherry‐oat aphid Rhopalosiphum padi (L.) on detached leaves and on leaves on intact plants of the winter host, bird cherry Prunus padus. There are no significant differences between the times taken to first parturition or the number of nymphs produced over a 6‐ or 18‐h period on intact plants or detached leaves. Stylet probing activities (i.e. total probe duration, xylem ingestion, the time to first phloem contact and phloem salivation) are also similar in the two situations.
A new genus of Staphylinidae (Coleoptera) from the Lower Cretaceous: the first fossil rove beetles from the Southern Hemisphere
Authors: SCHOMANN ANDREA , SOLODOVNIKOV ALEXEY
Published: January 2012

The fossil genus of rove beetles Apticaxgen.n. with two new species, A. volanssp.n. and A. solidussp.n., is described from the Nova Olinda Member of the Crato Formation in north‐eastern Brazil (Aptian–Albian, dated as 125–99.6 Ma old). Both species belong to the clade Staphylininae + Paederinae in the staphylinine group of subfamilies and are the first fossil true Staphylinidae to be described from the entire Southern Hemisphere. Although they resemble Paederinae, the low number of satisfactorily preserved characters do not allow definite placement of either A. volans or A. solidus in any of the subfamilies of Staphylinidae.
Renal allograft loss in the first post‐operative month: causes and consequences
Authors: Phelan Paul J. , O’Kelly Patrick , Tarazi Munir , Tarazi Nadim , Salehmohamed M. Ridhwaan , Little Dilly M. , Magee Colm , Conlon Peter J.
Published: January 2012

Phelan PJ, O’Kelly P, Tarazi M, Tarazi N, Salehmohamed MR, Little DM, Magee C, Conlon PJ. Renal allograft loss in the first post‐operative month: causes and consequences.

Abstract:  Early transplant failure is a devastating outcome after kidney transplantation. We report the causes and consequences of deceased donor renal transplant failure in the first 30 d at our center between January 1990 and December 2009. Controls were adult deceased donor transplant patients in the same period with an allograft that functioned >30 d. The incidence of early graft failure in our series of 2381 consecutive deceased donor transplants was 4.6% (n = 109). The causes of failure were allograft thrombosis (n = 48; 44%), acute rejection (n = 19; 17.4%), death with a functioning allograft (n = 17; 15.6%), primary non‐function (n = 14; 12.8%), and other causes (n = 11; 10.1%). Mean time to allograft failure was 7.3 d. There has been a decreased incidence of all‐cause early failure from 7% in 1990 to <1% in 2009. Patients who developed early failure had longer cold ischemia times when compared with patients with allografts lasting >30 d (p < 0.001). Early allograft failure was strongly associated with reduced patient survival (p < 0.001). In conclusion, early renal allograft failure is associated with a survival disadvantage, but has thankfully become less common in recent years.