bluuebunny/crossref_metadata_2025 huggingface dataset:
Attentional Bias Is Associated with Negative Emotions in Problematic Users of Social Media as Measured by a Dot-Probe Task
Authors: Zhao Jin , Zhou Zinuan , Sun Bo , Zhang Xinyuan , Zhang Lin , Fu Shimin
Published: December 2022

Objective: Social media (SM) have flourished and are affecting human lives on an unprecedented scale. Problematic social media use (PSMU) is a recently emerging problematic behavior that affects both physical and mental health. The purpose of this study was to investigate whether problematic users of SM display attentional bias (AB) toward SM-related cues, as well as the relationships between AB, the severity of PSMU, and negative emotions. Method: 1000 college students were recruited through classes and online forums and then tested using the Bergen Social Media Addiction Scale (BSMAS). Eventually, 60 participants, identified by the cut-off point for BSMAS, consented to participate in the study and were divided into two groups (i.e., a problematic use group and a typical use group). The severity of PSMU and negative emotions (i.e., anxiety, depression, social fear, and loneliness) were evaluated by self-report questionnaires. AB was assessed by an addiction Stroop task and a dot-probe task (DPT). Results: PSMU was found to be positively associated with anxiety (r = 0.28, p < 0.05), depression (r = 0.35, p < 0.01), and social fear (r = 0.38, p < 0.01), but not with loneliness (r = 0.19, p = 0.15). Participants with a tendency toward PSMU displayed AB toward SM-related cues in the DPT [F (1, 58) = 26.77, p < 0.001, ηp2 = 0.32], but not in the Stroop task [F (1, 58) = 0.61, p = 0.44, ηp2 = 0.01]. Moreover, AB toward SM-related stimuli was found to be positively correlated with the severity of PSMU (r = −0.51, p < 0.001), anxiety (r = −0.37, p < 0.01), depression (r = −0.51, p < 0.001), and social fear (r = −0.30, p < 0.05) in the DPT. Conclusions: Problematic users of SM show AB towards SM-related cues in the DPT, which is more reliable for assessing AB than the Stroop task. Moreover, it is suggested that in clinical interventions we should work to change the AB towards SM-related stimuli and improve negative emotions to decrease risks of PSMU.
Developing Ecological Thresholds for Nitrogen and Phosphorus in the Haihe River Basin in China
Authors: Wu Fan , Fang Yuan , Feng Mingfeng , Xie Zhicheng , Zhu Lin , Feng Jianfeng
Published: December 2022

Many aquatic ecosystems are eutrophicated due to human inputs of nitrogen and phosphorus. Therefore, it is now considered important to establish nutrient criteria to prevent eutrophication. In this study, the water quality and biological data for 26 stations in the lower reaches of the Haihe River Basin were collected. The total nitrogen (TN) and total phosphorus (TP) ecological thresholds were derived from the threshold indicator taxa analysis (TITAN) and frequency distribution approach. The results showed that the TN threshold was 1.8 mg/L based on the TITAN and the TP threshold was 0.039 mg/L based on the frequency distribution approach. The TITAN also identified 29 indicator species of TN and 28 indicator species of TP. Based on these criteria, we found 73% sites with TN concentrations higher than the 1.8 mg/L. As for TP, 58% sites exceeded 0.039 mg/L. This study showed that most of the downstream areas of the Haihe River are subject to relatively serious disturbances. This finding could provide implications for identifying the water quality traits of and generating protection strategies for the Haihe River in Tianjin.
A Bibliometric Analysis and Visualization of Decision Support Systems for Healthcare Referral Strategies
Authors: Aboelkhir Hesham Ali Behary , Elomri Adel , ElMekkawy Tarek Y. , Kerbache Laoucine , Elakkad Mohamed S. , Al-Ansari Abdulla , Aboumarzouk Omar M. , El Omri Abdelfatteh
Published: December 2022

Background: The referral process is an important research focus because of the potential consequences of delays, especially for patients with serious medical conditions that need immediate care, such as those with metastatic cancer. Thus, a systematic literature review of recent and influential manuscripts is critical to understanding the current methods and future directions in order to improve the referral process. Methods: A hybrid bibliometric-structured review was conducted using both quantitative and qualitative methodologies. Searches were conducted of three databases, Web of Science, Scopus, and PubMed, in addition to the references from the eligible papers. The papers were considered to be eligible if they were relevant English articles or reviews that were published from January 2010 to June 2021. The searches were conducted using three groups of keywords, and bibliometric analysis was performed, followed by content analysis. Results: A total of 163 papers that were published in impactful journals between January 2010 and June 2021 were selected. These papers were then reviewed, analyzed, and categorized as follows: descriptive analysis (n = 77), cause and effect (n = 12), interventions (n = 50), and quality management (n = 24). Six future research directions were identified. Conclusions: Minimal attention was given to the study of the primary referral of blood cancer cases versus those with solid cancer types, which is a gap that future studies should address. More research is needed in order to optimize the referral process, specifically for suspected hematological cancer patients.
Potential of Volunteering in Formal and Informal Medical Education—A Theory-Driven Cross-Sectional Study with Example of the COVID-19 Pandemic
Authors: Cerbin-Koczorowska Magdalena , Przymuszała Piotr , Kłos Michał , Bazan Dominika , Żebryk Paweł , Uruski Paweł , Marciniak Ryszard
Published: December 2022

Students’ volunteering is an effective way to manage health crises, including pandemics. Due to the limited capacity of the healthcare system at the time of the COVID-19 outbreak, the engagement of students in volunteering services seemed invaluable. Based on different teaching–learning theories, in this survey study, we aimed to evaluate the potential of the volunteering service project launched by the Poznan University of Medical Sciences during the COVID-19 pandemic as a learning opportunity for undergraduate healthcare students. The results indicate the potential of involving students in volunteering activities for educational purposes, as well as other values, including attitudes and professional identity development, which could be difficult to realize using traditional teaching methods. However, stimulating students’ reflectiveness seems necessary to reach its full educational effectiveness. Medical teachers should provide students with more opportunities for volunteering and service learning and consider making these a constant element of the curriculum beyond the COVID-19 pandemic.
Relationship between Oral Function and Support/Care-Need Certification in Japanese Older People Aged ≥ 75 Years: A Three-Year Cohort Study
Authors: Iwai Komei , Azuma Tetsuji , Yonenaga Takatoshi , Nomura Taketsugu , Sugiura Iwane , Inagawa Yujo , Matsumoto Yusuke , Nakashima Seiji , Abe Yoshikazu , Tomofuji Takaaki
Published: December 2022

The aim was to examine the relationships between oral functions and support/care-need certification in older people aged ≥ 75 years using the National Health Insurance (NHI) database system and data from Kani City, Gifu, Japan. In total, 732 older Japanese people aged ≥ 75 years who did not have support/care-need certification and underwent dental check-ups in Kani City in 2017 were followed up until 2020. Chewing state, tongue and lip function, and swallowing function were assessed by a self-administered questionnaire, an oral diadochokinesis test, and a repetitive saliva-swallowing test, respectively. The presence or absence of systemic diseases and of support/care-need certification was based on data collected by the NHI database. At follow up, 121 (17%) participants had support/care-need certification. The participants with support/care-need certification included more women (p < 0.001) and older people (p < 0.001); and had more hypertension (p = 0.003), musculoskeletal disorders (p < 0.001), pneumonia (p = 0.044), poor chewing state (p < 0.001), and poor swallowing function (p = 0.003) than those without support/care-need certification. Furthermore, the presence of support/care-need certification at follow up was associated with sex (woman: odds ratio [OR], 2.120; 95% confidence interval [CI], 1.354 to 3.317), age (OR, 1.203; CI, 1.139 to 1.270), chewing state (poor: OR, 2.534; CI, 1.409 to 4.557), and swallowing function (poor: OR, 2.372; CI, 1.248 to 4.510) at baseline. However, tongue and lip function were not associated with support/care-need certification. The results indicate that older Japanese people aged ≥ 75 years with a poor chewing state and poor swallowing function at baseline had a higher risk for support/care-need certification after three years.
How Can Resource-Exhausted Cities Get Out of “The Valley of Death”? An Evaluation Index System and Obstacle Degree Analysis of Green Sustainable Development
Authors: Zhuang Xinyu , Li Xin , Xu Yisong
Published: December 2022

Resource-based cities are suffering from resource scarcity and environmental deterioration. Spirit, vitality and prosperity are disappearing and cities have moved towards “the valley of death” in terms of urban development. This typically appears in environments where it is difficult to maintain sustainable development. Based on empirical analysis, a qualitative analysis method for the selection of evaluation indicators, as well as a quantitative analysis method for index weighting and principal component extraction for constructing a three-level evaluation index system of green development for coal-resource-exhausted cities, was adopted. This study also discussed the life cycle at different development stages of resource-based cities, including mature resource-based and growing resource-based cities. We further argued that the obstacle degree can act as an evaluation basis and make recommendations accordingly to improve the green development of cities. Through star-standard divisions and statistical analysis, it can be explicated that the increase in green development in the first stage is greater than that in the later stage, which is more obvious in cities with lower stars. The results also show the evolution trend and stability coefficient. There is no end in sight for urban green development, and this study can provide a new perspective to relieve the declining trend and promote green sustainable development.
Effectiveness of an Inpatient Virtual Reality-Based Pulmonary Rehabilitation Program among COVID-19 Patients on Symptoms of Anxiety, Depression and Quality of Life: Preliminary Results from a Randomized Controlled Trial
Authors: Rutkowski Sebastian , Bogacz Katarzyna , Czech Oliver , Rutkowska Anna , Szczegielniak Jan
Published: December 2022

Forms of rehabilitation for patients after COVID-19 are gaining interest. The purpose of this study was to investigate and compare an innovative in-hospital pulmonary rehabilitation programs augmented with training elements performed in virtual reality. This randomized controlled study included 32 patients enrolled in post-COVID-19 rehabilitation at a Public Hospital in Poland. The rehabilitation models included exercise capacity training on a cycle ergometer, breathing and general fitness workout, resistance training, and relaxation. The forms of training and relaxation differed between the groups: the experimental group employed virtual reality, and the control group used a traditional form of therapy. Exercise tolerance was assessed using a 6 min walk test (6 MWT), while psychological parameters were evaluated using the Hospital Anxiety and Depression Scale (HADS) and the brief World Health Organization Quality of Life Scale (WHOQOL-BRIEF). The analysis of the post-rehabilitation results showed a statistically significant improvement in both groups regarding depression (VR: 6.9 (3.9) vs. 4.7 (3.5), p = 0.008; CG: 7.64 (4.5) vs. 6.6 (4.8), p = 0.017) and anxiety (VR: 8.6 (4.6) vs. 5.6 (3.3), p < 0.001; CG: 9.57 (6.0) vs. 8 (4.8), p = 0.003). No statistically significant improvements in quality of life were noted in both groups. Moreover, the analysis showed a statistically significant improvement in the exercise capacity in both groups after completion of the rehabilitation program, expressed as a distance in the 6 MWT, as well as a statistically significant improvement in dyspnea in the VR group. To conclude, the analysis of the preliminary data revealed that a 3-week hospital-based pulmonary rehabilitation program for COVID-19 patients led to an improvement in exercise tolerance as well as a reduction in the symptoms of anxiety and depression. The virtual reality-based form of training delivery, despite its attractiveness, did not significantly affect patients’ performance.
Do Environmental Regulations Promote or Inhibit Cities’ Innovation Capacity? Evidence from China
Authors: Zeng Xiaowen , Jin Ming , Pan Shuang
Published: December 2022

The “Porter Hypothesis” proposes that appropriate environmental regulations would promote firm innovation. This study aims to build a theoretical model for illustrating the impact and mechanism of environmental regulation on urban innovation through a panel of 281 Chinese prefecture-level cities during 2003–2016. The results indicated that an increase in environmental regulation markedly suppressed the innovative capacity of Chinese cities during the sample period. This inhibitory effect is primarily transmitted through two mediating variables: lower regional fiscal revenue and reduced manufacturing output. Moreover, improved regional economic development level helps generate positive incentives for environmental regulation and mitigate its inhibitions to innovation. Environmental regulation and urban innovation might have a non-linear U-shape relation, with the former helping improve urban innovation capacity upon reaching a particular level.
Study of the Phytoextraction and Phytodegradation of Sulfamethoxazole and Trimethoprim from Water by Limnobium laevigatum
Authors: Stando Klaudia , Czyż Aleksandra , Gajda Magdalena , Felis Ewa , Bajkacz Sylwia
Published: December 2022

Phytoremediation is an environmentally friendly and economical method for removing organic contaminants from water. The purpose of the present study was to use Limnobium laevigatum for the phytoremediation of water from sulfamethoxazole (SMX) and trimethoprim (TRI) residues. The experiment was conducted for 14 days, in which the loss of the pharmaceuticals in water and their concentration in plant tissues was monitored. Determination of SMX and TRI was conducted using liquid chromatography coupled with tandem mass spectrometry. The results revealed that various factors affected the removal of the contaminants from water, and their bioaccumulation coefficients were obtained. Additionally, the transformation products of SMX and TRI were identified. The observed decrease in SMX and TRI content after 14 days was 96.0% and 75.4% in water, respectively. SMX removal mainly involved photolysis and hydrolysis processes, whereas TRI was mostly absorbed by the plant. Bioaccumulation coefficients of the freeze-dried plant were in the range of 0.043–0.147 for SMX and 2.369–2.588 for TRI. Nine and six transformation products related to SMX and TRI, respectively, were identified in water and plant tissues. The detected transformation products stemmed from metabolic transformations and photolysis of the parent compounds.
Cases of Maxillofacial Trauma Treated at Hospitals in a Large City in Northeastern Brazil: Cross-Sectional Study
Authors: Barreto Samuel Benson Lima , Castro Gustavo Garcia , Carvalho Ceci Nunes , Ferreira Meire Coelho
Published: December 2022

Maxillofacial trauma is associated with facial deformation, loss of function, emotional and social impacts, and high financial costs. This study investigated cases of maxillofacial trauma in a large Brazilian city through a cross-sectional study conducted at two public and two private hospitals. Primary data of 400 patients were collected through a questionnaire, clinical examination, and tomography. Statistical analysis at the 5% significance level was performed. Motorcycle accident was the major cause of trauma (41%); the most frequent trauma and treatment were mandibular fracture (24.3%) and surgery (71%), respectively. The female sex was more affected only regarding domestic accidents (p = 0.041) and falls (p < 0.001). Motorcycle accidents were more prevalent among 20 to 29 year-olds (p < 0.001), followed by physical aggression (p < 0.001) and sports accidents (p = 0.004). Falls were more frequent among 40 to 59 year-olds (p < 0.001). Mandibular fracture affected males and 20 to 29 year-olds more and was mainly associated with motorcycle accidents (48.2%) and physical aggression (22.7%) (p = 0.008). Nose fracture was more frequent in falls (29.6%), physical aggression (22.5%), and sports accidents (21.1%) (p < 0.001). Compound fracture was associated with motorcycle accidents (84.2%; p = 0.028). Maxillofacial trauma (mandibular, nasal, and zygomatic fractures) was associated with motorcycle accidents, physical aggression, and falls. Surgical treatment, hospital care, and public services were the most frequent.
Lactate Threshold and Psychomotor Fatigue Threshold in Hot Conditions: Suggestions for Soccer Players Participating in the Qatar World Cup 2022
Authors: Konefał Marek , Chmura Jan , Charmas Małgorzata , Kotowska Jadwiga , Błażejczyk Krzysztof , Chmura Paweł
Published: December 2022

The study aimed at finding relationships between lactate threshold and psychomotor fatigue threshold during incremental exercise in thermo-neutral climate conditions and conditions for the 2022 FIFA World Cup in Qatar simulated in an environmental test chamber. The study included 24 soccer players aged 21.02 ± 3.22 years old. The following procedures were performed: The incremental exercise test to mark lactate concentration—LA (mmol·l−1); Psychomotor test to determine choice reaction time; Designation of the lactate threshold (TLA) and psychomotor fatigue threshold (TPF). Climate conditions: The procedure was performed twice in the climatic chamber: (1) in thermo-neutral conditions—TNC (ambient temperature 20.5 °C and relative air humidity 58.7%), (2) after 7 days—in Qatar conditions—QC (28.5 ± 1.92 °C) and (58.7 ± 8.64%). It was confirmed that the TPF, which reflects the highest efficiency of CNS functioning, occurs at a higher running speed than the TLA. The temperature of 28.5 °C with 58.7% humidity, which is the lower limit of heat stress, causes the psychomotor fatigue threshold to appear at a lower running speed than in thermoneutral conditions. The data recorded in this work may help to understand the specificity of physiological and psychomotor reactions to various climatic conditions.
“Dignity as a Small Candle Flame That Doesn’t Go Out!”: An Interpretative Phenomenological Study with Patients Living with Advanced Chronic Obstructive Pulmonary Disease
Authors: Laranjeira Carlos , Dourado Marília
Published: December 2022

Long-term illness, such as chronic obstructive pulmonary disease (COPD), can expose people to existential suffering that threatens their dignity. This qualitative study explored the lived experiences of patients with advanced COPD in relation to dignity. An interpretative phenomenological approach based on lifeworld existentials was conducted to explore and understand the world of the lived experience. Twenty individuals with advanced COPD (GOLD [Global Initiative for Chronic Obstructive Lung Disease] stages III and IV) were selected using a purposive sampling strategy. In-depth interviews were used to collect data, which were then analysed using Van Manen’s phenomenology of practice. The existential experience of dignity was understood, in essence, as “a small candle flame that doesn’t go out!”. Four intertwined constituents illuminated the phenomenon: “Lived body–balancing between sick body and willingness to continue”; “Lived relations–balancing between self-control and belongingness”; “Lived Time–balancing between past, present and a limited future”; and “Lived space–balancing between safe places and non-compassionate places”. This study explains how existential life phenomena are experienced during the final phases of the COPD trajectory and provides ethical awareness of how dignity is lived. More research is needed to investigate innovative approaches to manage complex care in advanced COPD, in order to assist patients in discovering their inner resources to develop and promote dignity.
Seasonal Variation Characteristics and the Factors Affecting Plankton Community Structure in the Yitong River, China
Authors: Dong Ang , Yu Xiangfei , Yin Yong , Zhao Ke
Published: December 2022

To explore how environmental factors affected the plankton structure in the Yitong River, we surveyed the water environmental factors and plankton population in different seasons. The results showed high total nitrogen concentrations in Yitong River throughout the year, while the total phosphorus, water temperature (WT), and chemical oxygen demand in summer were significantly higher than those in other seasons (p < 0.05), and the dissolved oxygen (DO) concentrations and TN/TP ratio were significantly lower (p < 0.01) than those in other seasons. There was no significant seasonal change in other environmental factors. Cyanophyta, Chlorophyta, and Bacillariophyta were the main phytoplankton phylum, while Protozoa and Rotifera were the main zooplankton phylum. The abundance and biomass of zooplankton and phytoplankton in the summer were higher than those in other seasons. Non-Metric Multidimensional scaling methods demonstrated obvious seasonal variation of phytoplankton in summer compared to spring and winter, while the seasonal variation of the zooplankton community was not obvious. The results of the redundancy analysis showed that WT, DO and nitrate nitrogen were the main environmental factors affecting phytoplankton abundance. In contrast to environmental factors, phytoplankton was the main factor driving the seasonal variation of the zooplankton community structure. Cyanophyta were positively correlated with the changes in the plankton community.
Social Image of Old Age, Gendered Ageism and Inclusive Places: Older People in the Media
Authors: Sánchez-Román María , Autric-Tamayo Gadea , Fernandez-Mayoralas Gloria , Rojo-Perez Fermina , Agulló-Tomás María Silveria , Sánchez-González Diego , Rodriguez-Rodriguez Vicente
Published: December 2022

Ageism promotes the exclusion of older people from society by generating a negative image that they also internalize. The aim of this article is to investigate older people’s social self-image, through statements broadcast on a national Spanish radio program aimed at this group. A qualitative analysis was conducted for a random sample from the sound archive for the Radio Nacional de España program Juntos Paso a Paso (Together, Step by Step) (2008–2021), using codes based on the pillars and determinants of active ageing and the three dimensions of ageism. Intercoder agreement was calculated. There were significant findings regarding ageism, gendered ageism and ageing in place, with differences according to size of municipal area. The program in question can be considered a viable secondary source for the research aim. Ageism is most commonly manifested through implicit opinions and invisibilization in family and social contexts. Care activities play a notably central role in responses related to gendered ageism. In relation to ageing in place, older people prefer their habitual environment when they have moderate care needs and accept moves to nursing homes when their needs increase.
Health Outcomes in Women Victims of Intimate Partner Violence: A 20-Year Real-World Study
Authors: Clemente-Teixeira Maria , Magalhães Teresa , Barrocas Joana , Dinis-Oliveira Ricardo Jorge , Taveira-Gomes Tiago
Published: December 2022

Intimate partner violence is characterized by violent actions against a person perpetrated by his or her former or current partner, regardless of cohabitation. It most frequently affects women, and one of its most relevant outcomes is the health problems associated with the experience of repeated violence. Thus, the main objective of this study is to analyse the prevalence of health problems among women for whom there was a medical suspicion of being victims of intimate partner violence. The specific objectives are to analyse the prevalence of (a) health risk behaviours; (b) traumatic injuries and intoxications; (c) mental health conditions; and (d) somatic diseases. We conducted a real-world, retrospective, observational, cross-sectional and multicentric study based on secondary data analyses of electronic health records and health care register data in patients of the Local Healthcare Unit of Matosinhos (between 2001 and 2021). The identified data were extracted from electronic health records corresponding to the Health Insurance Portability and Accountability Act Safe Harbor Standard. Information was obtained considering the International Classification of Diseases, the International Classification of Primary Care, and the Anatomical Therapeutic Chemical Classification System, as well as clinical notes (according to previously defined keywords). Considering all information sources, 1676 cases were obtained. This number means that just 2% of the women observed at this health care unit were suspected of being victims of intimate partner violence, which is far from the known statistics. However, we found much higher rates of all health risk behaviours, trauma and intoxication cases, mental health conditions, and somatic disorders we looked for, when compared to the general population. Early detection of these cases is mandatory to prevent or minimize their related health outcomes.
A Life Dedicated to Climbing and Its Sequelae in the Fingers—A Review of the Literature
Authors: Pastor Tatjana , Schweizer Andreas , Andronic Octavian , Dietrich Léna G. , Berk Till , Gueorguiev Boyko , Pastor Torsten
Published: December 2022

Fingers of sport climbers are exposed to high mechanical loads. This work focuses on the fingers of a 52-year-old active elite climber who was the first in mankind to master 8B (V13), 8B+ (V14) and 8C (V15) graded boulders, bringing lifelong high-intensity loads to his hands. It is therefore hypothesized that he belongs to a small group of people with the highest accumulative loads to their fingers in the climbing scene. Fingers were analyzed by means of ultrasonography, X-rays and physical examination. Soft tissue and bone adaptations, as well as the onset of osteoarthritis and finger stiffness, were found, especially in digit III, the longest and therefore most loaded digit. Finally, this article aims to provide an overview of the current literature in this field. In conclusion, elite sport climbing results in soft tissue and bone adaptations in the fingers, and the literature provides evidence that these adaptations increase over one’s career. However, at later stages, radiographic and clinical signs of osteoarthritis, especially in the middle finger, seem to occur, although they may not be symptomatic.
The Effect of Nurse Support Programs on Job Satisfaction and Organizational Behaviors among Hospital Nurses: A Meta-Analysis
Authors: Kim Se Young , Cho Mi-Kyoung
Published: December 2022

The purpose of this study was to calculate the combined effect size of nurse support programs on job satisfaction among hospital nurses. The PICO framework was used in this methodological study of systematic review and meta-analysis. Using nine electronic databases of four international and five Korean databases and applying the eligibility criteria, articles published from database inception through October 2022 were collected. A total of 24 Korean and international articles were selected following the PRISMA guidelines. The keywords of nurse, mentoring (preceptorship, internship, or residency) program, and job satisfaction were selected based on the PICO. The checklists for RCTs and quasi-experimental studies provided in the Joanna Briggs Institute of Critical Appraisal Tool were used for the quality assessment. The coded data were analyzed using MIX 2.0 statistical software. We analyzed the combined effect sizes, heterogeneity, funnel plot, Egger’s regression test, Begg’s test, subgroup analyses, and univariate meta-regression. The overall effects of the program on job satisfaction and organizational behavior such as organizational commitment, interpersonal relationships, self-efficacy, motivation, burnout, and turnover intention for hospital nurses were statistically significant. The results of this study may explain the effect of the nurse support program on job satisfaction and organizational behaviors for hospital nurses.
Athlete Identity and Mental Health of Student Athletes during COVID-19
Authors: Antoniak Katherine , Tucker Clea , Rizzone Katherine , Wren Tishya A. L. , Edison Bianca
Published: December 2022

The purpose of our study is to evaluate athletic identity (AI) and mental health measures of youth and young adult athletes during the COVID-19 pandemic. This cross-sectional study recruited athletes aged 11–25 years from universities, high schools, and middle schools in California and New York. Participants were emailed a link to an anonymous, cross-sectional electronic survey. The measure included the athletic identity measurement scale (AIMS), the Patient Health Questionnaire-4 (PHQ-4), and demographic variables. Chi-square, Fisher’s Exact Test, and linear regression were used to examine the relationships between AI, symptoms of anxiety, and symptoms of depression by age, gender, and race. The survey was completed by 653 participants. AI was stratified by tertiary percentiles. The odds of positively scoring for symptoms of anxiety were 60% higher for participants in college compared with high school (OR: 1.60, 95% CI: [1.09, 2.35]). Conversely, the odds of scoring positively for symptoms of depression were 68% higher for participants in high school compared to college (OR: 1.68, 95% CI: [1.09, 2.59]). The odds of scoring positively for symptoms of depression were higher for athletes who scored as high AI, compared to those who scored as moderate (OR: 1.72, 95% CI: [1.11, 2.68]) or low (OR: 1.93, 95% CI: [1.20, 3.12]). The odds of scoring positively for symptoms of anxiety on the PHQ-4 were 3.2 times higher for participants who identified as female (OR: 3.19, 95% CI: [2.31, 4.41]), and the odds of scoring positively for symptoms of depression were 2.4 times higher for participants who identified as female (OR: 2.35, 95% CI: [1.56, 3.54]). Female athletes experienced symptoms of depression and anxiety at significantly higher rates than male athletes during the COVID-19 pandemic. High school students experienced fewer symptoms of anxiety, but greater symptoms of depression as compared to the collegiate group, while college students experienced greater odds of symptoms of anxiety. Athletes in the high AI group were more likely to report symptoms of depression than moderate or low identity groups. Female athletes reported lower AI than male athletes, but still had greater symptoms of anxiety and depression.
Evaluation Study of xMAP TIER Assay on a Microsphere-Based Platform for Detecting First-Line Anti-Tuberculosis Drug Resistance
Authors: Ou Xichao , Zhang Zhiguo , Zhao Bing , Song Zexuan , Wang Shengfen , He Wencong , Pei Shaojun , Liu Dongxin , Xing Ruida , Xia Hui , Zhao Yanlin
Published: December 2022

Early diagnosis of drug susceptibility for tuberculosis (TB) patients could guide the timely initiation of effective treatment. We evaluated a novel multiplex xMAP TIER (Tuberculosis-Isoniazid-Ethambutol-Rifampicin) assay based on the Luminex xMAP system to detect first-line anti-tuberculous drug resistance. Deoxyribonucleic acid samples from 353 Mycobacterium tuberculosis clinical isolates were amplified by multiplex polymerase chain reaction, followed by hybridization and analysis through the xMAP system. Compared with the broth microdilution method, the sensitivity and specificity of the xMAP TIER assay for detecting resistance was 94.9% (95%CI, 90.0–99.8%) and 98.9% (95%CI, 97.7–100.0%) for rifampicin; 89.1% (95%CI, 83.9–94.3%) and 100.0% (95%CI, 100.0–100.0%) for isoniazid; 82.1% (95% CI, 68.0–96.3%) and 99.7% (95% CI, 99.0–100.0%) for ethambutol. With DNA sequencing as the reference standard, the sensitivity and specificity of xMAP TIER for detecting resistance were 95.0% (95% CI, 90.2–99.8%) and 99.6% (95% CI, 98.9–100.0%) for rifampicin; 96.9% (95% CI, 93.8–99.9%) and 100.0% (95% CI, 100.0–100.0%) for isoniazid; 86.1% (95% CI, 74.8–97.4%) and 100.0% (95% CI, 100.0–100.0%) for ethambutol. The results achieved showed that the xMAP TIER assay had good performance for detecting first-line anti-tuberculosis drug resistance, and it has the potential to diagnose drug-resistant tuberculosis more accurately due to the addition of more optimal design primers and probes on open architecture xMAP system.
Lysmeral Exposure in Children and Adolescences Participating in the German Environmental Survey (2012–2015): Integrating Sex/Gender into Analysis
Authors: Fichter Sophie Ch. , Groth Katrin , Fiedler Nina , Kolossa-Gehring Marike , Dębiak Małgorzata ,   on behalf of the INGER Study Group
Published: December 2022

Comprehensive consideration of the biological and social diversities of sex and gender as well as their interdependencies is mostly missing in human biomonitoring (HBM) studies. Using the INGER sex/gender concept as theoretical background, we analyzed differences in exposure to lysmeral, a compound commonly found as a fragrance in cosmetics, personal care, and household products, in 2294 children and adolescents in Germany using decision tree, regression, and mediation analysis. The variables “sex assigned at birth” and “age”, as well as well as use of personal care products and fabric conditioner proved to have the highest explanatory value. Mediating effects of behaviour associated with societal gender expectations were observed, as the use of cosmetics correlated highly with lysmeral metabolites concentrations in girls between 6 and 17 years, with the strongest effect in adolescents between 14 and 17 years old. In the youngest age group (3–5 years) boys showed higher concentration of the metabolite tert-butylbenzoic acid (TBBA) compared to girls of the same age but only if TBBA urine concentrations were normalized on creatinine. Our study offers the first retrospective sex/gender assessment of HBM data. It demonstrates the possibilities to rethink and broaden sex/gender analysis in existing HBM-studies and highlights the need for inclusion of new sex/gender concepts in the design of new studies.
Young Adult Development Indicators for Indigenous and Non-Indigenous People: A Cross-National Longitudinal Study
Authors: Doery Elizabeth , Satyen Lata , Paradies Yin , Rowland Bosco , Bailey Jennifer A. , Heerde Jessica A. , Renner Heidi , Smith Rachel , Toumbourou John W.
Published: December 2022

Worldwide, Indigenous youth face ongoing challenges and inequalities. Increasing our understanding of life course patterns in Indigenous youth will assist the design of strategies and interventions that encourage positive development. This study aimed to increase understanding of resilience and positive development in Indigenous and non-Indigenous youth across Australia and the United States of America. The Australian sample comprised 9680 non-Indigenous and 176 Pacific Islander and Aboriginal and Torres Strait Islander peoples. The USA sample comprised 2258 non-Indigenous and 220 Pacific Islander, Native Hawaiian and Native American/American Indian peoples. Data were used to examine how Indigenous background, volunteering, and community involvement at average age 15 years (Grade 9) predicted five young adult positive development indicators: Year 12 (Grade 12) school completion, tertiary education participation, independent income, paid employment, and intimate relationship formation from age 18 to 28 years. Multilevel regression analyses revealed that while Indigenous youth showed slower increases in positive young adult development over time, when adjusting for socioeconomic disadvantage, there was a reduction in this difference. Moreover, we found that Grade 9 community involvement and volunteering were positively associated with young adult development for Indigenous and non-Indigenous youth. Findings indicate the importance of addressing structural inequalities and increasing adolescent opportunities as feasible strategies to improve positive outcomes for young Indigenous adults.
Integrating Remote Sensing and Street View Imagery for Mapping Slums
Authors: Najmi Abbas , Gevaert Caroline M. , Kohli Divyani , Kuffer Monika , Pratomo Jati
Published: December 2022

Mapping slums is vital for monitoring the Sustainable Development Goal (SDG) indicators. In the absence of reliable data, Remote Sensing (RS)-based approaches, particularly the Deep Learning (DL) methods, have gained recognition and high accuracies for slum mapping. However, using RS alone has its limitation in complex urban environments. Previous studies showed the added value of combining ground-level information with RS. Therefore, this research aims to integrate Remote Sensing Imagery (RSI) and Street View Images (SVI) for slum mapping. Jakarta city is the study area representing the challenge of distinguishing between slum and non-slum kampungs, and these kampungs accommodate approximately 60% of the population of Jakarta. This research compares the mapping results obtained by four DL networks: FCN-DK6 used only RSI, a VGG16 used only SVI, and two networks combined RSI and SVI (FCN-DK6-i and Modified FCN-DK6). Further, the Modified FCN-DK6 network was explored by integrating SVI at each convolutional layer, i.e., Modified FCN-DK6_1, Modified FCN-DK6_2, Modified FCN-DK6_3, Modified FCN-DK6_4, and Modified FCN-DK6_5. Experimental results demonstrate that combining RSI and SVI improves the accuracy, depending on how and at what level in the FCN network they are integrated. The Modified FCN-DK6_2 outperforms the rest in Modified FCN-DK6 experiments and FCN-DK6-i.
DNA Nanoflowers’ Amelioration of Lupus Symptoms in Mice via Blockade of TLR7/9’s Signal
Authors: Wang Jing , Gan Mingzhe
Published: December 2022

Inhibitory oligodeoxynucleotides (INH-ODN) can exert an immunomodulatory effect to specifically block TLR7 and TLR9 signaling in systemic lupus erythematosus (SLE). To extend the half-life of INH-ODN in vivo, the phosphorothioate backbone, instead of the native phosphodiester, is preferred due to its strong resistance against nuclease degradation. However, its incomplete degradation in vivo may lead to potential risk. To solve these problems and enhance the blockage of TLR7 and TLR9, we prepared highly compressed DNA nanoflowers with prolonged native DNA backbones and repeated INH-ODN motifs. Three therapeutic types of nanoflower, incorporating INH-ODN sequences, including IRS 661, IRS 869, and IRS 954, were prepared by rolling circle amplification and were subcutaneously injected into MRL/lpr mice. The TLR7 blocker of the IRS 661 nanoflower and the TLR9 antagonist of the IRS 869 nanoflower could decrease autoantibodies, reduce cytokine secretion, and alleviate lupus nephritis in mice. However, the IRS 954 nanoflower, the TLR7 and TLR9 dual antagonist, did not have additive or opposing effects on lupus nephritis but only showed a decrease in serum IFNα, suggesting that the TLR7 and TLR9 antagonist may have a competition mechanism or signal-dependent switching relationship. INH-ODN nanoflowers were proposed as a novel and potential therapeutic nucleic acids for SLE.
Primary Breast Angiosarcoma: Comparative Transcriptome Analysis
Authors: Rincón-Riveros Andrés , De la Peña Jairo , Rubiano Wilson , Olivella Fabio , Martinez-Agüero María , Villegas Victoria E.
Published: December 2022

Primary breast angiosarcoma, with de novo appearance and not associated with exposure to radiation or lymphedema, is a rare pathology representing less than 0.05% of the neoplasms related to this organ. The pathology is characterized by its aggressiveness, poor prognosis, and difficulties in its differential diagnosis. This article reports the case of a 55-year-old white woman with no family history of cancer, with a rapidly growing mass in the left mammary gland that ulcerates and bleeds. It is confirmed as primary breast angiosarcoma by immunostaining in the tumor tissue for CD31, CD34, and FLI-1. In addition, a sample of neoplastic and healthy tissues is collected from the patient for RNA sequencing; the results are contrasted with a tissue sample from a patient with Luminal A subtype of breast cancer, as well as data from other cases of angiosarcoma available in public databases. These findings revealed a genetic profile associated with the immune and inflammatory response in the patient’s sample when compared to available angiosarcoma data; these molecular patterns are consistent with other recent studies. Due to the rarity of the disease, the studies carried out on each patient contribute to the expanding knowledge of the etiology and molecular pathways that are still partially known and continue to be the subject of research. Aside from a comparative transcriptome study, this article aims to provide an update on the state of knowledge about this disease.
Anion-Controlled Synthesis of Novel Guanidine-Substituted Oxanorbornanes
Authors: Barešić Luka , Margetić Davor , Glasovac Zoran
Published: December 2022

The cycloaddition of simple alkyl-substituted guanidine derivatives is an interesting approach toward polycyclic superbases and guanidine-based organocatalysts. Due to the high nucleophilicity of guanidines, an aza-Michael reaction with dienophiles is more common and presents a huge obstacle in achieving the desired synthetic goal. Our preliminary investigations indicated that the proton could act as a suitable protecting group to regulate the directionality of the reaction. To investigate the role of the protonation state and type of anion, the reactivity of furfuryl guanidines with dimethyl acetylenedicarboxylate was explored. Furfuryl guanidines showed a strong reaction dependence on the nucleophilicity of the counterion and the structure of guanidine. While the reaction of DMAD with the guanidinium halides provided products of an aza-Michael addition, Diels–Alder cycloaddition occurred if non-nucleophilic hexafluorophosphate salts were used. Depending on the structure and the reaction conditions, oxanorbornadiene products underwent subsequent intramolecular cyclization. A tendency toward intramolecular cyclization was interpreted in terms of the pKa of different positions of the guanidine functionality in oxanorbornadienes. New polycyclic guanidines had a slightly decreased pKa in acetonitrile and well-defined geometry suitable for the buildup of selective sensors.
Substrate Recognition Properties from an Intermediate Structural State of the UreA Transporter
Authors: Sanguinetti Manuel , Silva Santos Lucianna Helene , Dourron Juliette , Alamón Catalina , Idiarte Juan , Amillis Sotiris , Pantano Sergio , Ramón Ana
Published: December 2022

Through a combination of comparative modeling, site-directed and classical random mutagenesis approaches, we previously identified critical residues for binding, recognition, and translocation of urea, and its inhibition by 2-thiourea and acetamide in the Aspergillus nidulans urea transporter, UreA. To deepen the structural characterization of UreA, we employed the artificial intelligence (AI) based AlphaFold2 (AF2) program. In this analysis, the resulting AF2 models lacked inward- and outward-facing cavities, suggesting a structural intermediate state of UreA. Moreover, the orientation of the W82, W84, N279, and T282 side chains showed a large variability, which in the case of W82 and W84, may operate as a gating mechanism in the ligand pathway. To test this hypothesis non-conservative and conservative substitutions of these amino acids were introduced, and binding and transport assessed for urea and its toxic analogue 2-thiourea, as well as binding of the structural analogue acetamide. As a result, residues W82, W84, N279, and T282 were implicated in substrate identification, selection, and translocation. Using molecular docking with Autodock Vina with flexible side chains, we corroborated the AF2 theoretical intermediate model, showing a remarkable correlation between docking scores and experimental affinities determined in wild-type and UreA mutants. The combination of AI-based modeling with classical docking, validated by comprehensive mutational analysis at the binding region, would suggest an unforeseen option to determine structural level details on a challenging family of proteins.
The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion
Authors: Gavshina Alexandra V. , Solovyev Ilya D. , Savitsky Alexander P.
Published: December 2022

Photoswitchable fluorescent proteins (FPs) have become indispensable tools for studying life sciences. mSAASoti FP, a biphotochromic FP, is an important representative of this protein family. We created a series of mSAASoti mutants in order to obtain fast photoswitchable variants with high brightness. K145P mSAASoti has the highest molar extinction coefficient of all SAASoti mutants studied; C21N/K145P/M163A switches to the dark state 36 times faster than mSAASoti, but it lost its ability to undergo green-to-red photoconversion. Finally, the C21N/K145P/F177S and C21N/K145P/M163A/F177S variants demonstrated a high photoswitching rate between both green and red forms.
The Soluble Fms-like Tyrosine Kinase-1 Contributes to Structural and Functional Changes in Endothelial Cells in Chronic Kidney Disease
Authors: Schulz Annika , Drost Carolin Christina , Hesse Bettina , Beul Katrin , Brand Marcus , Di Marco Giovana Seno
Published: December 2022

Endothelial cells are a critical target of the soluble Fms-like tyrosine kinase-1 (sFlt-1), a soluble factor increased in different diseases with varying degrees of renal impairment and endothelial dysfunction, including chronic kidney disease (CKD). Although the mechanisms underlying endothelial dysfunction are multifactorial and complex, herein, we investigated the damaging effects of sFlt-1 on structural and functional changes in endothelial cells. Our results evidenced that sera from patients with CKD stiffen the endothelial cell cortex in vitro, an effect correlated with sFlt-1 levels and prevented by sFlt-1 neutralization. Besides, we could show that recombinant sFlt-1 leads to endothelial stiffening in vitro and in vivo. This was accompanied by cytoskeleton reorganization and changes in the endothelial barrier function, as observed by increased actin polymerization and endothelial cell permeability, respectively. These results depended on the activation of the p38 MAPK and were blocked by the specific inhibitor SB203580. However, sFlt-1 only minimally affected the expression of stiffness-sensitive genes. These findings bring new insight into the mechanism of action of sFlt-1 and its biological effects that cannot be exclusively ascribed to the regulation of angiogenesis.