bluuebunny/crossref_metadata_2025 huggingface dataset:
The impact of public health emergency governance based on artificial intelligence
Authors: Zheng Hao , Peng Chuanyuan
Published: July 2022

To optimize the data clustering effect of public health emergencies, an application research on social governance ability under public health emergencies based on artificial intelligence is proposed. First, the firefly optimization algorithm is used to collect the information data of the social governance ability of public health emergencies, establish a unified format, and save it. Then, artificial intelligence technology is used to mine the correlation of clustering data, and on this basis, a learning model integrating global structure information and local structure information is established. Finally, the social governance model under public health emergencies is established. The experimental results show that the design method has high clustering accuracy, regularization cross index, and adjusted rand index (ARI) index. This shows that the design method can improve the social governance ability of data fusion clustering and improve the social governance ability.
Evaluation of the etiological and clinical characteristics of pediatric central diabetes insipidus
Authors: Gasimova Elnare , Berberoğlu Merih , Özsu Elif , Aycan Zehra , Uyanık Rukiye , Bilici Esra , Ceran Ayşegül , Şiklar Zeynep
Published: July 2022

Objectives

Central diabetes insipidus (CDI) is a rare but important disease of varying etiology that poses challenges in diagnosis and follow-up. Identifying diagnostic difficulties in patients with CDI will help ensure an optimal approach to their management and follow-up. This study aimed to characterize the clinical and etiological characteristics of CDI in pediatric patients.

Methods

We analyzed the admission and follow-up data of CDI patients aged 0–18 years who were followed in our center between 2010 and 2019.

Results

The study included 56 patients with a mean age at diagnosis of 7.92 ± 5.11 years and symptom duration of 8.65 ± 21.3 months. The patients were grouped by etiology into those with organic causes, such as structural anomalies, tumors, and trauma (group 1, n=41) and other causes (group 2, n=15). The prevalence of idiopathic CDI was 16%. At least one pituitary hormone deficiency was detected in 60.7%, the most common being thyroid stimulating hormone deficiency. Patients in group 1 had a higher mean age at diagnosis, shorter symptom duration, and higher frequency of other pituitary hormone deficiencies compared to group 2. Additionally, germinoma was detected 1 year subsequent to normal MRI findings at diagnosis and another patient was diagnosed with Langerhans cell histiocytosis (LCH) 5 years after diagnosis. All patients responded well to replacement therapies, but two patients with germinoma died during follow-up.

Conclusions

In the pediatric age group, intracranial organic pathologies are an important etiology of CDI, and despite a short symptomatic period, determining the cause may be challenging and prolonged. Patients presenting at a young age with a long history of symptoms and no other pituitary hormone deficiency are unlikely to have organic CDI. However, organic causes such as LCH should be evaluated at all ages. Patients with idiopathic disease are candidates for further etiological studies, and repeated cranial imaging is important during follow-up.
Association of methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms with vitamin B12 deficiency and adverse perinatal outcomes among pregnant women of rural South India – a cross sectional longitudinal study
Authors: Barney Anitha M. , Danda Sumita , Cherian Anne G. , Aronraj Jency , Jayaprakash Lavanya , Abraham Vinod J. , Christudass Christhunesa S. , Marcus Tobey A.
Published: July 2022

Objectives

To determine the occurrence of MTHFR gene polymorphisms and to study their association with vitamin B12 deficiency and adverse perinatal outcomes among a cohort of pregnant women from Kaniyambadi block, Tamil Nadu.

Methods

120 consecutive pregnant women who were ≤20 weeks of gestational age from the 82 villages of Kaniyambadi block were recruited. Genomic DNA was isolated from the peripheral blood. PCR amplification was done followed by Sangers sequencing. Maternal and neonatal outcomes were extracted. Data was entered and analysed.

Results

Our study found the occurrence of c.1298A>C variant in homozygous state in 14.2% and c.677C>T heterozygous state in 15%. Sanger sequencing of exon 7 identified another pathogenic variant c.1262G>T in heterozygous state in two of them. Both the mothers who harboured that variant had preterm delivery and one of them gave birth to a low-birth-weight neonate. In the entire cohort, 5% of the mothers had abortion, 4.2% of them had preterm delivery and 8.8% of the neonates had low birth weight. Presence of c.1298A>C or c.677C>T variants were associated with vitamin B12 deficiency [Pearson Chi squared value (χ2)=7.9 and 7.6 respectively; p=0.02]. Heterozygous pathogenic variant c.1262G>T was associated with both adverse maternal [χ2=11.5; p=0.001] and neonatal [χ2=18.3; p=0.009] outcomes.

Conclusions

MTHFR gene polymorphisms could be associated with several adverse perinatal outcomes and vitamin B12 deficiency. Further larger studies are needed to prove the pathogenicity of c.1262G>T variant on pregnancy.
Unsteady film cooling characteristics of the high-pressure turbine shroud with blade rotation in an aero-engine
Authors: Kou Zhihai , Bao Zihao , Li Guangchao , Yin Xunyan
Published: July 2022

Three-dimensional unsteady numerical simulations were conducted to investigate the detailed film cooling mechanism of the high-pressure turbine shroud with the first-stage turbine blade and guide vane for an aero-engine under the high-speed rotation of blades and rotor-stator interaction. The slip mesh was used to realize the relative motion between the rotating blade and the stationary turbine shroud. It is found that the coolant jet is alternately influenced by the hot mainstream, tip clearance leakage flow and leakage vortex due to the high rotational speed of blades. The film cooling characteristics of the turbine shroud significantly present an unsteady and periodic flow and heat transfer phenomenon. The insufficient cooling margin for film holes at the upstream of the blade leading edge can occur not only under high blowing ratios due to the coolant jet liftoff, but also at low blowing ratios due to the insufficient coolant flow rate as a result of the high exit pressure. A novel shroud cooling structure with coolant supply by the added throttle chamber is put forward, and expected to provide better thermal protection for the high-pressure turbine shroud near the leading edge of blades with no extra increase in the total mass flow rate of coolant.
Inter-Animal Variability in Activity Phase Is Constrained by Synaptic Dynamics in an Oscillatory Network
Authors: Anwar Haroon , Martinez Diana , Bucher Dirk , Nadim Farzan
Published: July 2022

The levels of voltage-gated and synaptic currents in the same neuron type can vary substantially across individuals. Yet, the phase relationships between neurons in oscillatory circuits are often maintained, even in the face of varying oscillation frequencies. We examined whether synaptic and intrinsic currents are matched to maintain constant activity phases across preparations, using the lateral pyloric (LP) neuron of the stomatogastric ganglion (STG) of the crab,Cancer borealis. LP produces stable oscillatory bursts on release from inhibition, with an onset phase that is independent of oscillation frequency. We quantified the parameters that define the shape of the synaptic current inputs across preparations and found no linear correlations with voltage-gated currents. However, several synaptic parameters were correlated with oscillation period and burst onset phase, suggesting they may play a role in phase maintenance. We used dynamic clamp to apply artificial synaptic inputs and found that those synaptic parameters correlated with phase and period were ineffective in influencing burst onset. Instead, parameters that showed the least variability across preparations had the greatest influence. Thus, parameters that influence circuit phasing are constrained across individuals, while those that have little effect simply co-vary with phase and frequency.
The distribution and life history of Mysis gaspensis O.S. Tattersall, 1954 (Crustacea, Mysida): An almost endemic, nektonic component of Atlantic Canada estuarine and coastal ecosystems
Authors: Dadswell Michael J.
Published: July 2022

Mysids (Mysida) or opossum shrimp are nektonic Crustacea found worldwide in freshwater, hypogean, coastal marine or deep-sea habitats. A poorly known, highly localized species Mysis gaspensis O.S. Tattersall, 1954 is found only in Atlantic Canada and Maine, USA. During spring to autumn, localized populations form aggregations at low tide in littoral estuarine environments but individuals also occur at littoral coastal sites. In boreal habitats, this mysid has a univoltine, semelparous life cycle. Beginning in March-April, juveniles are released from the female marsupium at 2.0-2.5 mm total length (TL). Growth in TL is linear (r2 = 0.90), and growth in wet weight is exponential (b = 2.96). Females attain 14.1-16.5 mm TL and males 13.9-25.5 mm TL, by November when they begin maturation.During November-January, penultimate and ultimate males and females migrate seaward where copulation is presumed to occur. Males die after copulation. Development of young during winter results in a mixture of embryo development stages in individual female marsupia by spring. Mean brood size is 59 embryos (range 32-83). Females reoccupy estuarine habitats during March-April and release their young. Females survive after release of young and attain a maximum length of ~20.0 mm TL, but most die or are predated upon by late July. Juveniles and adults of some populations form highly visible aggregations during low tide in the shallow water of estuaries occupying sites with low velocity currents (12-41 cm/s) and near the limit of salinity (0.5-19.0). Aggregations range in size up to 38,000 individuals but the population in some estuaries is often scattered at low tide in small groups or individuals on the down-stream side of rocks or underwater structures.Keywords: aggregations; brood size; growth; maturation; opossum shrimp; reproduction
REMEMBERING THE MARINE ECOLOGY LABORATORY, BEDFORD INSTITUTE OF OCEANOGRAPHY, 1965-1987: AN HISTORICAL AND PERSONAL PERSPECTIVE
Authors: Gordon Donald C.
Published: July 2022

The Marine Ecology Laboratory (MEL) was one of the principal federal scientific laboratories at the Bedford Institute of Oceanography (BIO) in Dartmouth, NS. Created in 1965 as an independent laboratory under the Fisheries Research Board of Canada, it grew out of the previous Atlantic Oceanographic Group with the broad mandate to study the structure and dynamics of marine ecosystems supporting marine fisheries. With time, it developed a well-rounded program of basic and applied ecological research and earned an international reputation for excellence. In 1987, it fell victim to a major reorganization of the Department of Fisheries and Oceans, driven by Ottawa managers, and was closed despite widespread protest from the scientific community. However, once the dust had settled from this unfortunate incident and, despite declining resources, ecological research at BIO continued to flourish under a new organizational structure.
Six years of Sentinel-2 archive of Slovenia
Authors: Pehani Peter , Veljanovski Tatjana , Kokalj Žiga , Oštir Krištof
Published: July 2022

The Sentinel-2 is a high resolution optical satellite mission, developed by the European Space Agency (ESA) for the European Commission. Currently the mission has two satellites in orbit: Sentinel-2A from 23rd June 2015, and Sentinel-2B from 7th March 2017. Revisit time for this twin constellation is five days. The twin satellites represent a very stable data source, but for the first six operational months (i.e. the second half of the 2015). From the very start of the mission, the Research Centre of the Slovenian Academy of Sciences and Arts (ZRC SAZU) is collecting its own archive of Sentinel-2 data for the extent of the Republic of Slovenia. The data is saved in its original processing state (Level-1C) and in four levels of processing. The archive has now been operating for six full years, which is a good milestone for a statistical overview. We analyse the archive of Sentinel-2 data of Slovenia from several aspects at the country level. A special focus is placed on cloud cover, as only cloud-free data can give an impression of the actual spatial and temporal usability of optical data.
Modeling procedure within the mass valuation of real estate in Slovenia
Authors: Ulbl Melita , Glavica Andrej
Published: July 2022

The mass valuation of real estate in Slovenia is based on valuation models, which are made based on realised real estate market data and illustrate the functioning of the real estate market. The system is based on valuation models that simulate the behaviour of the real estate market and allow a statistically reliable assessment of market value. Models are formed using statistical methods of processing real estate market data. This paper presents the quality verification process and preparation of real estate market data. These data are used to design evaluation models or modelling, which consists of time adjustment of prices or rents, zoning, levelling, calibration, and analysis of quality parameters. Finally, the critical information of the modelling process made in the context of real estate mass valuation in Slovenia is summarised.
The Relationship of Character Accentuations and Emotional Disorders in the Context of the COVID-19 Pandemic
Authors:   Novosibirsk State Pedagogical University, Andronnikova Olga O. , Turova Natalia V. ,   Novosibirsk State Pedagogical University
Published: July 2022

The article presents data on the study of the relationship between character accentuations and emotional disorders in the adult population in the context of the COVID-19 pandemic. The methods used to study this relationship are listed. The analysis of the results of the study allowed us to determine the types of character accentuations that are prerequisites for the occurrence of emotional disorders. The study revealed a type of character accentuation that disposes to the absence of anxiety and allows you to remain satisfied with your living conditions against the background of the events of the COVID-19 pandemic. Recommendations of psychological self-help in conditions of self-isolation are given.
The Relationship of Personal Characteristics and Motivation of Achievement among Students of Defectology
Authors:   Novosibirsk State Pedagogical University, Veterok Ekaterina V.
Published: July 2022

The article presents an analysis of the relationship between personal characteristics and achievement motivation among students of speech pathologists. It is noted that the motivation of achievement is determined by personal factors that determine the activity of an individual in social reality. The characteristic of personal characteristics of students is given on the basis of the analysis of foreign and domestic works. A number of definitions of motivation and achievement motivation are given. The concept of achievement motivation in relation to personal characteristics is defined. The article presents the results of an empirical study of the relationship between personal characteristics and achievement motivation among students of defectology, according to which students of defectology, prone to emotional stability, demonstrate a pronounced desire to achieve results. Defectology students with pronounced introversion show independence in the process of achieving results.
The Study of the Phenomenon of Social Apathy and its Manifestation is Society
Authors:   Novosibirsk State Pedagogical University, Veselkina Nadezhda E.
Published: July 2022

The article presents a theoretical analysis of the phenomenon of social apathy, the factors influencing its formation and development are highlighted. Social apathy is considered both as a state of the individual and as a state of a social group. The results of a study aimed at studying the severity of social apathy in society are also presented. The study was conducted using the methodology “Scale of Apathyˮ A. A. Zolotareva and an author’s questionnaire aimed at studying social life. It is concluded that the level of apathy is at an average level, but tends to increase. The subjects are interested in the events taking place in the city and the world, but this does not affect the increase in the sense of ownership in the development of society and the country, which is an important indicator of the presence of social apathy in the group. At the same time, in general, people prefer to stay at home longer than usual, do not actively participate in social events and do not participate in the development of their city. The prospects for further development and study of social apathy are outlined, due to the insufficiency of both theoretical and practical research.
Phobias, Fears and Anxiety in Adolescents with Autism Spectrum Disorder
Authors:   Novosibirsk State Pedagogical University, Kirillina Angelica E. , Novolodskaya Galina V. ,   Novosibirsk State Pedagogical University, Smyshlyaeva Ekaterina O. ,   Novosibirsk State Pedagogical University
Published: July 2022

This article examines such concepts as fears, phobias and anxiety, and describes the types and causes of their occurrence. In addition, the problems of fears, phobias and anxiety in adolescents with disabilities and autism spectrum disorder are discussed. The aim of the work is to investigate phobias, fears and anxiety in adolescents with autism spectrum disorder. An analysis of theoretical sources that address fears, phobias, and anxiety in normotypical adolescents and adolescents with autism spectrum disorder is presented. The conclusion identifies fears and phobias in adolescents with disabilities and adolescents with autism spectrum disorder.
The Factors Affecting Fraud Prevention in BOS Fund Management
Authors: Ramadhani Rima Sari , Lawita Nadia Fathurrahmi , Anriva Della Hilia
Published: July 2022

The purpose of this research was to determine the effect and analyze the factors that affect the prevention of fraud in the management of BOS Funds. The method in this research was quantitative method. The sample in this study was schools that received the School Operational Assistance Fund (BOS) in Pekanbaru City, the research respondents were school principals and BOS fund. The sampling technique in this study used a random sampling technique with a total of 74 respondents. The analytical technique used in this study was regression analysis using SPSS version 24. From the results of the partial study, it is known that the SPIP, Organizational Culture, Anti-Fraud Awareness, and Whistleblowing System factors had a positive and significant effect on fraud prevention in the management of BOS Funds.
Profile of Pedagogical Content Knowledge (PCK) of Accounting Subject Teachers at State Vocational High Schools
Authors: Mulasiwi Cut Misni , Kholifaturrohmah Ramita
Published: July 2022

Pedagogical content knowledge (PCK) merupakan kompetensi spesifik guru yang merupakan perpaduan antara pengetahuan materi ajar (konten) dan cara mengajar (pedagogis) yang berkembang seiring waktu dan pengalaman. Tujuan  penelitian  ini adalah menganalisis profil kemampuan PCK guru mata pelajaran akuntansi Sekolah Menengah Kejuruan Negeri di Kabupaten Banyumas. Penelitian ini merupakan penelitian deskriptif untuk mendeskripsikan kemampuan PCK guru mata pelajaran akuntansi. Metode dalam penelitian ini menggunakan pendekatan kualitatif, yaitu dengan cara mengumpulkan data, reduksi data, penyajian data, dan kesimpulan yang diperoleh melalui observasi, wawancara, dan dokumentasi. Teknik penentuan sampel menggunakan sampling jenuh. Subyek penelitian ini adalah guru akuntansi Sekolah Menengah Kejuruan Negeri di Kabupaten Banyumas yang berjumlah 26 guru. Adapun simpulan hasil data kualitatif melalui pengumpulan data wawancara tiap responden adalah guru mengintegrasikan materi ajar (konten) dan cara mengajar (pedagogik) dengan baik. Di dalam proses pembelajaran, guru selalu memikirkan pembelajaran yang efektif dengan menggunakan fasilitas seperti komputer, LCD proyektor, dan internet diintegrasikan menggunakan model dan media pembelajaran yang cukup bervariasi dengan disesuaikan materi. Namun, ada beberapa yang perlu ditingkatkan, di antaranya kemampuan guru dalam melaksanakan diskusi kelompok secara efektif, menguasai materi seperti subject matter expert, serta mengatasi kesulitan belajar siswa.
Potential ovarian toxicity and infertility risk following targeted anti-cancer therapies
Authors: Rosario Roseanne , Cui Wanyuan , Anderson Richard A
Published: July 2022

Unlike traditional chemotherapy agents which are generally cytotoxic to all cells, targeted anti-cancer therapies are designed to specifically target proliferation mechanisms in cancer cells but spare normal cells, resulting in high potency and reduced toxicity. There has therefore been a rapid increase in their development and use in clinical settings, including in curative-intent treatment regimens. However, the targets of some of these drugs including kinases, epigenetic regulatory proteins, DNA damage repair enzymes and proteasomes, have fundamental roles in governing normal ovarian physiology. Inhibiting their action could have significant consequences for ovarian function, with potentially long-lasting adverse effects which persist after cessation of treatment, but there is limited evidence of their effects on reproductive function. In this review, we will use literature that examines these pathways to infer the potential toxicity of targeted anti-cancer drugs on the ovary.

Lay summary

Compared to traditional chemotherapy agents, anti-cancer therapies are thought to be highly effective at targeting cancer cells but sparing normal cells, resulting in reduced drug side effects. However, many of processes within the cells that these drugs affect are also important for the ovary to work normally, so suppressing them in this way could have long-lasting implications for female fertility. This review examines the potential toxicity of anti-cancer therapies on the ovary.
Optical coherence microscopy allows for quality assessment of immature mouse oocytes
Authors: Fluks Monika , Tamborski Szymon , Szkulmowski Maciej , Ajduk Anna
Published: July 2022

In brief

Optical coherence microscopy is a label-free and non-invasive imaging technique capable of 3D subcellular structure visualization. Here we show that this method allows for quality assessment of immature mouse oocytes based on their chromatin conformation and can be a valuable addition to the toolkit used in assisted reproduction procedures.

The success of assisted reproductive technologies, and particularly in vitro maturation, is tightly linked to the quality of oocytes. Therefore, there is a need for robust, reliable, and easy-to-assess biomarkers of oocyte developmental competence. Microscopy techniques visualizing oocyte intracellular structure could provide such biomarkers. However, fluorescence imaging methods, applied frequently in biology and allowing for detailed structural and dynamic studies of single cells, require fluorescent tags to visualize cellular architecture and may cause short- and long-term photo-damage. On the other hand, traditional light microscopy, although relatively non-invasive, does not provide detailed structural information. Optical coherence microscopy (OCM) is a promising alternative, as it does not require sample pre-processing or labelling and can provide 3D images of intracellular structures. Here we applied OCM to assess the chromatin conformation of immature mouse oocytes, a feature that corresponds with their transcriptional status and developmental competence and cannot be examined by traditional light microscopy. We showed that OCM distinguished oocytes with so-called non-surrounded nucleoli (NSN) and surrounded nucleoli (SN) chromatin conformation with very high sensitivity and specificity and that OCM scanning did not decrease the quality of oocytes. Finally, we cross-referenced OCM data with the oocyte ability to undergo normal nuclear and cytoplasmic maturation and proven that indeed oocytes scored with OCM as NSN mature less effectively than oocytes scored as SN. Our results suggest that OCM may be a valuable addition to the imaging toolkit used in assisted reproduction procedures.
Dynamics of Ragweed Pollen Concentration in the Air of Ivano-Frankivsk
Authors: Melnychenko Halyna , Hrushka Viktoriia , Ivanova Оlha
Published: July 2022

The article updates the conduct of aeropalynological monitoring in Ukraine because today, there are only a few studies in the country that cannot be automatically extrapolated to other regions, as the diversity of climatic zones affects the duration of pollination and pollen grains in the air. Furthermore, this type of research is needed due to the increasing number of people suffering from pollen allergies (hay fever).The research was conducted in the city of Ivano-Frankivsk (western Ukraine) from early February to mid-October 2019, using a gravimetric Durham pollen collector installed on the roof of the Faculty of Natural Sciences of Vasyl Stefanyk Precarpathian National University (24 m from the ground). The pollen grains were counted using an Olympus CX-300 light microscope (magnification x 400). The main goal was to study the aeropalynological spectrum of the city and the dynamics of concentrations of ragweed pollen grains (the most important plant allergen).The study determined the aeropalynological spectrum of Ivano-Frankivsk and provided data on pollination of the following taxa: Betula, Quercus, Acer, Fraxinus, Pinus, Juglans, Carpinus, Alnus, Populus, Urticaceae, Poaceae, Artemisia, and Ambrosia; conducted a comparative characterization with the spectra of the cities of Vinnytsia (central Ukraine) and Zaporizhia (southern Ukraine); determined the beginning and end of pollination of ragweed and the peak of its flowering, as well as conducted a comparative description with last year's data.Seeing an alarming trend, there is a need to continue to conduct aeropalynological monitoring to create calendars of sawing and alleviate the course of allergies in people prone to it.
Weak approximations of the Wright–Fisher process and related problems
Authors:   , Mongirdaitė Gabrielė
Published: July 2022

Wright–Fisher proceso silpnosios aproksimacijos ir susiję uždaviniai
Sarkoidozės eigos ypatumai ir jos raidą lemiantys veiksniai
Authors:   , Aleksonienė Regina
Published: July 2022

Peculiarities of the course of sarcoidosis and factors determining its development
Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA
Authors:   Institute of Animal Biology NAAS, Lviv, Ukraine, Kozak M. R. , Petruh I. M. ,   Institute of Animal Biology NAAS, Lviv, Ukraine;, Vlizlo V. V. ,   Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine;
Published: July 2022

Vaccination is the best method to prevent the spread of infectious diseases, its disadvantages are side effects. Potentially safe DNA, RNA or protein molecules possess antigenic properties, but are low-immunogenic and therefore require conjugation with an adjuvant. The aim of the research was to evaluate Chitosan (CS) potency as an adjuvant and compare its effectiveness depending on the route of drug administration. The experiments were carried out on 3 groups of BALB/c mice. Mice of the first group were injected subcutaneously with 20 µl of a mixture of CS (3.3 mg/kg) and BSA (1.7 mg/kg). The mixture of CS and BSA at the same doses and volume was administered orally to mice of the second experimental group. The third group – control – unvaccinated mice. Anti-BSA antibody levels were measured by ELISA. Aspartate aminotransferase, alanine aminotransferase activity and cholesterol, creatinine and urea levels were determined in the serum. It was found that both subcutaneous and mucosal immunizations provided a 2-fold increase in anti-BSA antibody titers against the background of maintaining all biochemical blood parameters at the level of the physiological norm. However, AST activity in the serum of oral-immunized mice was elevated as compared to subcutaneous-immunized mice. Serum cholesterol level in the group of subcutaneously immunized mice and creatinine and urea levels in both experimental groups were reduced compared to the control. It is concluded that oral immunization with CS is the optimal route for antigen-specific IgG antibody response induction.
Tumor biomarkers CEA, CA19.9, CA15.3 and AFP levels in the serum of patients with COVID-19
Authors:   Department of Biotechnology, Faculty of Science, Sebha University, Libya, Ali Abubaker H. , Yaqub Abdullah H. ,   Department of Zoology, Faculty of Science, Sebha University, Libya;, Faraj Ihssin A. ,   Department of Zoology, Faculty of Science, Sebha University, Libya
Published: July 2022

Early diagnosis is very important to reduce morbidity and mortality in COVID-19 infected patients. The aim of this study was to detect of tumor antigens CEA, CA19.9, CA15.3, and AFP and to compare their levels in the serum of 69 COVID-19 patients and 69 healthy individuals who did not have COVID-19. The levels of CEA, CA19.9, CA15.3, and AFP in the serum were measured using ELISA. The levels of the tumor biomarkers in the serum of COVID-19 patients were found to be 7.74 ± 4.65 ng/ml for CEA, 29.33 ± 16.35 U/ml for CA19.9, 23.24 ± 13.48 U/ml for CA15.3 and 7.46 ± 5.57 ng/ml for AFP, while in the serum of healthy control patients 9.73 ± 43.58 ng/ml for CEA, 20.66 ± 11.1 for CA19.9, 19.64 ± 10.99 U/ml for CA15.3, and 3.83 ± 9.20 ng/ml for AFP, indicating no differences in the levels of the studied tumor biomarkers in the two experimental groups. It is concluded that tumor biomarkers CEA, CA19.9, CA15.3, and AFP cannot be used as effective screening tools for patients with COVID-19.
Thrombomodulin and von willebrand factor as markers of endothelial dysfunction in patients with chronic kidney disease
Authors:   Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine, Mykhaloiko I. S. , Yatsyshyn R. I. ,   Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine;, Cherniuk N. V. ,   Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine;, Humeniuk M. Ja. ,   Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine;
Published: July 2022

The aim of research was to study the levels of thrombomodulin (TM) and von Willebrand factor (VWF) in the serum and urine of patients with chronic kidney disease (CKD)as diagnostic markers of endothelial dysfunction. The study involved 140 patients with CKD. The clinical diagnosis was determined based on standard methods of patients examination according to the kidney diseases classification and protocols of CKD patients management. The concentrations of TM and VWF in serum and urine were quantified by ELISA. A generalized endothelial dysfunction in the vessels of the whole body, including the kidneys and high concentration of TM and FVF in the serum and urine of patients with a diabetic nephropathy have been found. The concentration of TM and VWF in the serum of patients with a chronic glomerulonephritis was at the same level as in the serum of healthy individuals, while those in urine significantly exceeded the control values, indicating endothelial damage in the glomeruli of the kidneys due to exposure to pro-inflammatory cytokines. In our opinion, the studied markers will contribute to the timely diagnosis of endothelial dysfunction in patients with CKD and to the development of criteria for prescribing antiplatelet agents in glomerular kidney disease.
Application of gold nanoparticles to determine spermine in the presence of other polyamines
Authors:   R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Yanish Yu. V. , Prylutskyi M. P. ,   R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Zaletok S. P. ,   R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Mukha Yu. P. ,   O. O. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv;
Published: July 2022

The development of methods for the detection of polyamines in biological fluids is essential to improve early diagnosis and treatment of patients with prostate cancer. One of the promising areas is the use of noble metal nanoparticles. According to the literature data, there is no methodological approach have been developed to reliably distinguish spermine from other polyamines, in particular, from their acetylated forms and related compounds present in biological fluids. The paper presents the results of spectrophotometric determination of spermine both alone and in the presence of putrescine, spermidine or urea in the urine using gold nanoparticles. The results of the experiments proved that the developed method is suitable for the selective determination of spermine. It was shown that the presence of spermidine, putrescine, acetylated forms of polyamines or carbamide does not affect the results of the analysis.
Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel
Authors:   Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Lootsik M. D. , Manko N. O. ,   Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;, Bilyy R. O. ,   Danylo Halytsky National Medical University, Lviv, Ukraine, Lutsyk (Jr.) M. M. ,   Danylo Halytsky National Medical University, Lviv, Ukraine, Stoika R. S. ,   Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
Published: July 2022

Chitosan is biocompatible and biodegradable natural biopolymer widely applied in various fields of biology, medicine, and pharmacy, however, its effects significantly depend on the degree of polymerization (DP) and the degree of deacetylation (DDA) of polymer chains. Evaluation of the chitosan chain diversity by DP requires the use of a highly expensive method of high-performance size exclusion chromatography. The aim of our study was to determine the molecular weight profile of chitosan specimens by the use of electrophoresis in a porosity step gradient polyacrylamide gel and to evaluate the efficacy of this method in monitoring the purification of chitosan fragments and its derivatives. Two types of step gradient porosity gels were used: 1) gels of layers with acrylamide concentration 2.5, 3.5, 5.0, 10.0, 15.0, 20.0 % w/v for native chitosan or its high molecular fragments; 2) gels of layers with acrylamide concentration 2.5, 5.0, 10.0, 15.0, 20.0, 25.0 % w/v for low molecular chitosan fragments. The main amount of molecules from the chitosan pool was localized in the type 1 gel in the region of 550-40 kDa and distributed among three bands, which in different samples differed significantly in percentage. Electrophoresis of chitosan fragments fractionated by gel permeation chromatography provided a clear separation of medium molecular weight fragments (50–400 kDa) in type 1 gel and of low molecular weight fragments (3–40 kDa) in type 2 gel. Thus the method of chitosan electrophoresis in a step-gradient porosity of polyacrylamide gel was developed which permits to characterize the molecular weight profile of chitosan specimens polymer chains and is effective in monitoring the isolation of chitosan fragments by gel penetration chromatography of molecular weights from 3 to 400 kDa.
Mary
Authors: Pinto Raphael Colvara
Published: July 2022

The way in which a culture understands itself and its relationship with the sacred shapes not only religious life, but also a way of being in the world. The same can be said in the opposite direction: the religious sphere can be captured in some anthropomorphism to justify a position that excludes. Taking this premise into account, we can look at the position of women in the early church, the roles they played, and how and why the relevance of their performance was minimized through misogyny that occurs in different contexts of Christianity as a historical construction. By misogyny we understand a posture that engenders forms of discourse and practice that transform women into objects. Anchored in religious discourse in order to legitimize male hegemony, misogyny established a stereotype justifying female inferiority and submission. By understanding woman in the role Mary had as a woman and mother in the history and discourse of salvation, specifically in the incarnation, we can propose a positive perspective that goes beyond history and corrects some of the distortions of historicism with regard to the role of women.
Narradores e seus leitores em Memorial do Convento e A Viagem do Elefante
Authors: Campos Valéria Hernandorena Monteagudo de
Published: July 2022

Neste artigo, faz-se a análise dos narradores de Memorial do Convento e A Viagem do Elefante, de José Saramago, por meio da comparação entre os métodos narrativos que aproximam leitor e narradores. Em comum, há a recorrência a recursos estilísticos, como a metalinguagem e as marcas da oralidade, que buscam estabelecer uma comunicação com o leitor. No entanto, em Memorial do Convento, encontra-se um escritor de fôlego, cujas estratégias exigem repertório e releitura. Em A Viagem do Elefante, a linguagem é menos sofisticada e as reflexões do narrador vão além da consciência crítica, pois ele explica os fatos e correlaciona-os, a fim de ampliar o alcance de sua mensagem. Para a realização das análises, utiliza-se amparo crítico de Hutcheon (1991), Adorno (2008), Bakhtin (2010), Benjamin (2010), Genette (1979) e Friedman (2002), assim como de parte da fortuna crítica dedicada às metaficções historiográficas de Saramago, principalmente Cerdeira (2018), Raoni (2002) e Cruz (2013).
Panorama do Segundo Império, “Laivos Reacionários” e projeto moderno de história
Authors: Conte Tiago
Published: July 2022

O objetivo deste artigo é avaliar as estratégias utilizadas por Nelson Werneck Sodré na elaboração de Panorama do Segundo Império, publicado originalmente em 1939. Como primeiro trabalho de Sodré dedicado aos estudos históricos do Brasil, esse livro é anterior a seu alinhamento com os marcos teóricos do marxismo, quando o autor publicou suas obras mais conhecidas. Por esse motivo, Sodré não reeditou aquele trabalho nas décadas seguintes, assim como aposentou grande parte de sua bibliografia lançada até a década de 1940. No caso de Panorama do Segundo Império, as principais referências utilizadas por Sodré são intelectuais que pertencem ao campo autoritário do pensamento político e social brasileiro, como Azevedo Amaral e Oliveira Viana. Ao mesmo tempo, percebe-se que os conceitos apreendidos naqueles autores são empregados para analisar o período sem se limitar às cronologias lineares e ações dos personagens políticos da época, aspecto em que as reflexões de Reinhart Koselleck nos auxiliam a compreender os procedimentos historiográficos de Sodré.
Lexical means of expressing emotivity and evaluation in theprose by Gennady Yushkov
Authors: Bazhenova Olga N.
Published: July 2022

Introduction. The paper deals the description of some lexical means of expressing emotivity and evaluation in the prose by Gennady Yushkov (1932–2009).
Materials and Methods. The article is based on the texts of the novels and short stories by G. A. Yushkov. The author employs the method of component analysis, it defines such components of connotative meaning as parametric, emotional-evaluative, and figurative. It applies a structural method when describing models of individual phraseological units of the author.
Results and Discussion. Emotivity and evaluation in the prose by Gennady Yushkov is expressed by various lexical means. Disapproval of the subject of speech is conveyed by commonly used pejoratives and individual phraseological units of the author, created according to the models of ordinary phrases in a figurative sense, lexical repetitions in the replicas of the characters. Negative evaluation and negative emotions of the speaker are also expressed by figurative verbs, while the intensity component in the connotative meaning of the word becomes a prerequisite for the development of the evaluation component. Conceptually significant for the works by G. A. Yushkov is the component of approval in the connotative meaning of the Komi ethnonym. Such qualities of a Komi person as bringing things to the end, sincerity, purposefulness, perseverance, the ability to feel nature and navigate in the forest, as well as modesty.
Conclusion. The lexical means of expressing emotiveness and evaluation in the prose by Gennady Yushkov are quite diverse They are based on both the commonly used resources of the Komi folk language and the author’s own linguistic creativity.
Representation of the morbid state of a person in the phraseology of Russian and Mordovian dialects on the territory of the Republic of Mordovia
Authors: Maslova Alina Yu. , Mochalova Tatiаna I. , Levina Mariiа Z.
Published: July 2022

Introduction. The paper considers phraseological units that characterize the morbid state of a person in Russian and Moksha dialects on the territory of the Republic of Mordovia. The purpose of the study is to present the semantic characteristics of dialect phraseological units, analyze their component composition, and identify axiological and cultural-historical ideas of dialect speakers in the region.

Materials and Methods. Various techniques and research methods were used in the work: a descriptive method, a comparative method, a method of component analysis, a method of contextual analysis, a method of linguistic and cultural interpretation. The study was conducted on the material of phraseological dictionaries of Russian dialects of the Republic of Mordovia and the Mordovian (Moksha and Erzya) languages.

Results and Discussion. The study found that in the Russian and Moksha dialects of the Republic of Mordovia there are stable combinations known from ancient times, which name diseases and their symptoms, various painful conditions of a person. It was revealed that the languages of different types use phraseological units, which include somatisms, zoonyms, phytonyms, names of various objects, which reflects the features of a person’s figurative thinking, associative perception of the surrounding reality. A comprehensive study of phraseological units that characterize the morbid state of a person in the languages of different types on the territory of one region contributes to the comprehension of folk mentality, revealing the specifics of the national linguistic picture of the world of the Russian and Mordovian peoples, and allows us to trace the general and specific features of the linguistic community.

Conclusion. The results of the study indicate that phraseological units are actively used in the compared dialects of the Russian and Moksha languages, denoting a person’s morbid condition and representing the characteristic features of the image of the disease in different cultures.
Еxpression of conditionality in compound sentences of the Udmurt language (semantic and structural features)
Authors: Timerkhanova Nadezhda N.
Published: July 2022

Introduction. In the Udmurt language, compound sentences with subordinate clauses are very productive. The means of communication in such syntactic constructions are conjunctions, allied combinations and, in some cases, correlates. The same conjunctions help convey different semantics. The purpose of this paper is to identify conditional, conditional-temporal, conditionally causal semantic relations expressed with the help of conjunctions, allied combinations, and other linguistic means, as well as the structural features of such syntactic constructions on the material of the Udmurt language.
Materials and Methods. The research was based on the examples borrowed from Udmurt fiction. The author employed a set of research methods, such as descriptive; partial and continuous sampling; contextual analysis; transformation. The use of these methods made it possible to consider the specifics of the units under consideration on specific linguistic material.
Results and Discussion. The article deals with the widely represented compound sentences with conditional, conditional-temporal and conditionally causal clauses in the Udmurt language. It has been established that sentences of a repeating real condition and consequence often combine the properties of conditional and temporary sentences; constructions with a single condition or consequence in the form of the present tense combine conditional and causal sentences, in the form of the future tense combine both conditional-temporal and conditional; in the form of the past tense combine sentences that have only the meaning of time or reason, the meaning of the condition is erased. The type of specific semantic relationships at the level of the deep structure of the sentence can be determined by the conjunction, the marker of recurrence, as well as the form of the multiple aspect of the verb.
Conclusion. In the Udmurt language, the conditional-temporal meaning, as a rule, is expressed in complex sentences of a repeating real condition with the help of conjunctions ke, kuke ‘if (when)’; conditionally causal meaning is expressed in sentences of a single real condition with the conjunctions bere, dyrja ‘if (as)’, in which the connection between two non-repeating phenomena is expressed. The semantics of a compound sentence may depend not only on the conjunction used, but also on the type-time form of the verb-predicate, the semes of singleness or repeatability, additional lexical markers of singleness and multiplicity. The exact definition of meaning and contamination is possible only at the level of the deep structure of the sentence.
Vuelco del fique (Furcraea macrophylla) y sus repercusiones agronómicas
Authors: Ortiz-González Daniel
Published: July 2022

Introducción. El vuelco se conoce como la caída de plantas, fenómeno que afecta el rendimiento y la calidad de las cosechas en diversos cultivos. En Colombia se ha evidenciado el vuelco en plantaciones de fique (Furcraea spp.), pero se han estudiado poco sus efectos. Objetivo. Determinar las repercusiones del vuelco en el crecimiento de las plantas y en la producción de fibra de fique. Materiales y métodos. Se evaluaron veinte plantas erguidas y veinte plantas con vuelco bajo un diseño completamente al azar, en un cultivo de cuarenta años en Totoró, Cauca, Colombia, en marzo de 2021, a las que se les evaluó el crecimiento, el rendimiento y la longitud de fibra (LF), además, se cuantificó el porcentaje de vuelco y mortalidad a través de un muestreo aleatorio de 166 plantas. Resultados. La incidencia de vuelco fue del 58 %, de las plantas volcadas un 23 % murieron a causa de la pudrición del tallo. El vuelco afectó el crecimiento, ya que redujo un 10, 23, 14, 39 y 10,5 % el diámetro de tallo (DT), la longitud de hoja (LH), el ancho de hoja (AH), el número total de hojas (NTH) y la relación longitud y ancho de hoja (RLA), respectivamente, comparadas con plantas erguidas. La longitud de fibra y el rendimiento disminuyeron en un 24 y 56 %, respectivamente. Conclusiones. El vuelco causó una mayor afectación en la producción de fibra que la reportada por el virus de la macana, enfermedad más limitante del cultivo. Estos hallazgos pusieron en evidencia la importancia de buscar e implementar estrategias de manejo como aporte a la sostenibilidad de la cadena en el país.