bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation and Characterization of Extruded Yam Starch–Soy Protein Isolate Complexes and Their Effects on the Quality of Dough
Authors: Shi Miaomiao , Dong Xuena , Cheng Yanqiu , Ji Xiaolong , Liu Yanqi , Yan Yizhe
Published: January 2023

Extrusion is a method of processing that changes the physicochemical and rheological properties of starch and protein under specific temperature and pressure conditions. In this study, twin-screw extrusion technology was employed to prepare yam starch–soy protein isolate complexes. The structure and properties of the complexes and their effects on the quality of dough were studied. The results showed changes in the X-ray diffraction, rheology, and in vitro digestibility of the complexes. The extruded starch–protein complex formed an A+V-type crystal structure with the addition of soy protein isolate. A small amount of soy protein isolate could improve the complex’s viscoelasticity. As the content of soy protein isolate increased, the content of slow-digesting starch and resistant starch in the complexes increased, and the digestibility decreased. The microstructure of the dough indicated that the network structure of the puffed yam starch–protein complex dough was more uniform than that of the same amount of puffed yam starch. The moisture distribution of the dough showed that with the addition of extruded flour, the closely bound water content of the dough increased, and the weakly bound water content decreased. The hardness, gumminess, chewiness, and resilience of the dough decreased. In conclusion, extruded starch–protein complexes can improve dough quality and provide technical support for the broad application of yam.
New Generalized Hermite–Hadamard–Mercer’s Type Inequalities Using (k, ψ)-Proportional Fractional Integral Operator
Authors: Desta Henok Desalegn , Nwaeze Eze R. , Abdi Tadesse , Mijena Jebessa B.
Published: January 2023

In this paper, by using Jensen–Mercer’s inequality we obtain Hermite–Hadamard–Mercer’s type inequalities for a convex function employing left-sided (k, ψ)-proportional fractional integral operators involving continuous strictly increasing function. Our findings are a generalization of some results that existed in the literature.
On the Validation of a Fractional Order Model for Pharmacokinetics Using Clinical Data
Authors: Mtshali Sinenhlanhla , Jacobs Byron A.
Published: January 2023

This study aims to validate the hypothesis that the pharmacokinetics of certain drug regimes are better captured using fractional order differential equations rather than ordinary differential equations. To support this research, two numerical methods, the Grunwald–Letnikov and the L1 approximation, were implemented for the two-compartment model with Michaelis–Menten clearance kinetics for oral and intravenous administration of the drug. The efficacy of the numerical methods is verified through the use of the method of manufactured solutions due to the absence of an analytic solution to the proposed model. The model is derived from a phenomenological process leading to a dimensionally consistent and physically meaningful model. Using clinical data, the model is validated, and it is shown that the optimal model parameters select a fractional order for the clearance dynamic for certain drug regimes. These findings support the hypothesis that fractional differential equations better describe some pharmacokinetics.
Event Horizon and Environs (ETHER): A Curated Database for EHT and ngEHT Targets and Science
Authors: Ramakrishnan Venkatessh , Nagar Neil , Arratia Vicente , Hernández-Yévenes Joaquín , Pesce Dominic W. , Nair Dhanya G. , Bandyopadhyay Bidisha , Medina-Porcile Catalina , Krichbaum Thomas P. , Doeleman Sheperd , Ricarte Angelo , Fish Vincent L. , Blackburn Lindy , Falcke Heino , Bower Geoffrey , Natarajan Priyamvada
Published: January 2023

The next generation Event Horizon Telescope (ngEHT) will observe multiple supermassive black hole (SMBH) candidates down to a few tens of mJy, and profoundly transform our understanding of the local SMBH population. Given the impossibility of large-area high-resolution millimeter surveys, multi-frequency spectral energy densities (SEDs), and models are required to both identify source samples tailored to specific science goals, and to predict the feasibility of detection of individual interesting sources. Here, we present the Event Horizon and Environs (ETHER) source and SED model database whose primary use is to enable the selection and optimization of targets for EHT and ngEHT science. The living ETHER database currently consolidates 1.6 million black hole mass estimates, ∼15,500 milliarcsec-scale radio fluxes, ∼14,000 hard X-ray fluxes (expected to grow by factor ≳40 with the eROSITA data release) and SED information as obtained from catalogs and database queries, the literature, and our own new observations. Jet and accretion flow models are fit to individual SEDs in an automated way in order to predict the ngEHT observable fluxes from the jet base and accretion inflow. The database can be filtered by parameters or cross matched to a user source list, with the automated SED fitting models optionally fine tuned by the user. We have identified an initial ngEHT ‘gold sample’ for jet base studies and potentially black hole shadows; this sample will grow significantly in the coming years. While the ngEHT requires and will best exploit the ETHER database, six (eleven) ETHER sources have already been observed (scheduled) with the EHT in 2022 (2023), and the database has wide ranging applications in galaxy and black hole mass evolution studies.
Accidental Injury or “Shaken Elderly Syndrome”? Insights from a Case Report
Authors: Bugelli Valentina , Campobasso Carlo Pietro , Feola Alessandro , Tarozzi Ilaria , Abbruzzese Arturo , Di Paolo Marco
Published: January 2023

Subdural haemorrhage (SDH) as result of a traumatic brain injury (TBI) is a common cause of death in cases of fatal physical abuse. Since intracranial bleeding is a common finding in elderly due to age-related intracranial changes or increasing prevalence of anticoagulant medication, differential diagnosis between inflicted and non-inflicted head injury is challenging. A case of an elderly woman’s death caused by TBI is reported. Autopsy showed multiple polychromatic bruises and a frontoparietal hematoma with bilateral subacute SDH. History excluded paraphysiological or pathological non-traumatic conditions that could justify SDH, while iatrogenic factors only played a contributory role. Since polychromatic bruises distributed on the face, the upper extremities and the chest were consistent with forceful grasping/gripping or repeated blows and SDH can form in absence of impact or by mild/minor blows, SDH was considered the result of repeated physical abuses. Differential diagnosis between traumatic and non-traumatic SDH is still challenging for forensic pathologists. As largely accepted in the pediatric population and occasionally described also in adults, however, violent shaking should be also considered as a possible mechanism of SDH—especially in elderly who do not have any sign of impact to the head.
Novel Approaches to Enhancing Sustainable Adhesive System Solutions in Contemporary Book Binding: An Overview
Authors: Pasanec Preprotić Suzana , Vukoje Marina , Petković Gorana , Rožić Mirela
Published: January 2023

This paper contributes to eco-efficient and sustainable book binding production. Higher book binding manufacturing efficiency—with less waste and reduce energy consumption—has been achieved with higher inputs of natural biodegradable sources into graphic arts materials through the eco-labeled paper grades and the use of eco-advanced adhesive system solutions. Nowadays, scientific sources on non-toxic polymers and resins, combined with current scientific knowledge and production development, are closely related to sustainability. Hence, advanced and improved adhesive system solution technologies should fulfill the needs of suppliers and customers who are involved in the International Framework for ISO/TC130 workflows. These strategic partnerships provide possibilities in the context of “closed-loop recycling models”, which spark and advance the discussions of stakeholders. It is very important that the novel engineered biodegradable adhesive system solutions provide productivity-increasing and cost-effective solution performances by saving money and improving the performed binding activities. Without doubt, the task of the scientific community is to continue to provide responsive and comprehensive approaches to fulfilling stakeholders’ specific needs through standardized quality assurance, with the emphasis on book-binders.
Study on Heat Resistance of Peony Using Photosynthetic Indexes and Rapid Fluorescence Kinetics
Authors: Li Zhijun , Ji Wen , Hong Erman , Fan Zhen , Lin Bangyu , Xia Xuanze , Chen Xia , Zhu Xiangtao
Published: January 2023

To investigate the effects of high-temperature stress on the chlorophyll fluorescence induction kinetics of peony and to determine indicators for the rapid screening of varieties responding to high temperatures, three four-year-old peony variety, ‘Fengdanbai’, ‘Huhong’, and ‘Yinhongqiaodui’, were selected as materials. The photosynthetic curves (Pn-PAR) and fast chlorophyll fluorescence curves (OJIP curves) of peony leaves were measured at different times under high-temperature stress conditions (40 °C), the changes in the photosynthetic characteristics of different peony varieties under high-temperature stress were analyzed, and the heat tolerance of peony was evaluated. The results showed that ‘Huhong’ grew well within 16 days, while all of the other varieties showed obvious wilting at 6–9 days. High temperatures damaged the structure and function of the photosystem of peony leaves, indicating that the maximum net photosynthetic rate (Pnmax), apparent quantum efficiency (AQE), maximum photochemical efficiency (Fv/Fm), and photosynthetic performance index (PIABS) all tended to decrease under high-temperature stress, while the rate of closing the PS II reaction center (Mo) and the absorption per reaction center (ABS/RC), the capture (TRo/RC), and the dissipation (Dio/RC) of light energy per reaction center showed an overall increasing trend. The ability to cope with high-temperature stress differed among varieties, and the heat tolerance was determined to be in the descending order of ‘Fengdanbai’ < ‘Yinhongqiaodui’ < ‘Huhong’. The correlation analysis among the parameters and the analysis of the morphological change patterns in peony leaves concluded that PIABS, Dio/RC, and Mo could be used as indicators of peony tolerance to high-temperature stress. The results of the study can provide a basis for the screening of heat-tolerant peony species and peony heat defense in the Jiangnan area.
Combining Contamination Indices and Multivariate Statistical Analysis for Metal Pollution Evaluation during the Last Century in Lacustrine Sediments of Lacu Sărat Lake, Romania
Authors: Ganea Iolanda-Veronica , Bălc Ramona , Begy Robert-Csaba , Tanțău Ioan , Gligor Delia Maria
Published: January 2023

Integrated study of both water and sediment in lakes provides important information regarding the human impact on the environment. The current work is focused on the correlation between age, source, composition, and degree of human intervention over the last 178 years and health impact of sediments from Lacu Sărat Lake (Romania), one of the most important balneo-climateric resorts in the country. The novelty relies on the fact that this is the first time the temporal patterns of metal contamination and the human health effects associated with the metal exposure from sediment core samples have been assessed. The sediment contamination status was determined by evaluating several indices, such as the enrichment factor, geo-accumulation index, metal pollution index, and potential ecological risk index, etc. Results showed a significant accumulation of Cd, Cr, As and Ni and a major contribution of Pb, Zn, Cd, Hg, Cr as well as Cu to the potential acute toxicity. The sediment quality guidelines emphasized a risk concerning the life and proper development of benthic organisms in Lacu Sărat Lake. Moreover, the incidental ingestion lifetime carcinogenic risk values for As and Cr suggest a potential risk of developing cancer. A strong human impact was observed especially between 1950 and 1990, which can be attributed to the rapid economic growth and intensive industrial development strategies pursued by the communist political regime in Romania.
The Use of a Technology Acceptance Model (TAM) to Predict Patients’ Usage of a Personal Health Record System: The Role of Security, Privacy, and Usability
Authors: Alsyouf Adi , Lutfi Abdalwali , Alsubahi Nizar , Alhazmi Fahad Nasser , Al-Mugheed Khalid , Anshasi Rami J. , Alharbi Nora Ibrahim , Albugami Moteb
Published: January 2023

Personal health records (PHR) systems are designed to ensure that individuals have access and control over their health information and to support them in being active participants rather than passive ones in their healthcare process. Yet, PHR systems have not yet been widely adopted or used by consumers despite their benefits. For these advantages to be realized, adoption of the system is necessary. In this study, we examined how self-determination of health management influences individuals’ intention to implement a PHR system, i.e., their ability to actively manage their health. Using an extended technology acceptance model (TAM), the researchers developed and empirically tested a model explaining public adoption of PHRs. In total, 389 Saudi Arabian respondents were surveyed in a quantitative cross-sectional design. The hypotheses were analysed using structural equation modelling–partial least squares (SEM-PLS4). Results indicate that PHR system usage was influenced by three major factors: perceived ease of use (PEOU), perceived usefulness (PU), and security towards intention to use. PHR PEOU and PHR intention to use were also found to be moderated by privacy, whereas usability positively moderated PHR PEOU and PHR intention to use and negatively moderated PHR PU and PHR intention to use. For the first time, this study examined the use of personal health records in Saudi Arabia, including the extension of the TAM model as well as development of a context-driven model that examines the relationship between privacy, security, usability, and the use of PHRs. Furthermore, this study fills a gap in the literature regarding the moderating effects of privacy influence on PEOU and intention to use. Further, the moderating effects of usability on the relationship between PEOU, PU, and intention to use. Study findings are expected to assist government agencies, health policymakers, and health organizations around the world, including Saudi Arabia, in understanding the adoption of personal health records.
Aircraft Noise Reduction Strategies and Analysis of the Effects
Authors: Xie Jinlong , Zhu Lei , Lee Hsiao Mun
Published: January 2023

In this study, six aircraft noise reduction strategies including the optimization of aircraft type, regulation of night flight number, optimization of flight procedure, modification of operating runway, land use planning and installation of sound insulation windows were proposed to alleviate the harmful impact of aircraft noise on the local area and population near Guangzhou Baiyun International Airport (BIA) in China. The effects of all proposed strategies except for land use planning and sound insulation windows were simulated and analyzed using CadnaA software. The results indicate that these noise reduction strategies have their own advantages and each of them can serve as an effective noise reduction measure for different applications. For instance, the replacement of noisy aircraft with low-noise aircraft can simultaneously reduce the area and population exposed to a high noise level, while the optimization of flight procedure can only reduce the population exposed under relatively low noise levels (70 ≤LWECPN ≤ 75 dB). Nevertheless, the modification of operating runway is more effective in reducing the population suffering under high noise levels (LWECPN > 85 dB). Among these strategies, reducing the number of night flights is found to be most effective in reducing the overall noise-exposed area and population. Additionally, with the assistance of noise mapping, proper land use planning was suggested according to national standards, and the installation of sound insulation windows with different sound reduction grades can be determined for different areas impacted by the aircraft noise of BIA. It is believed that the results of this study can be applied as a reference in selecting suitable noise reduction strategies to improve the acoustic environment of a specific airport.
A Comparative Study of the Fatigue of the Lower Extremities According to the Type of Shoes Worn When Firing a 10 m Air Pistol
Authors: Zhang Yuxi , Zhang Ze , Kim Sukwon , Kim Youngsuk
Published: January 2023

The purpose of this study was to compare the differences in shooting performance, physical stability, and lower extremity muscle fatigue in high-level shooters wearing shooting shoes and sports shoes, and to determine the causes. Eight high-level 10 m air pistol shooters wore shooting shoes and sports shoes in a simulated shooting qualification experiment (60 rounds in 75 min) and we recorded shooting scores, pressure centers (COP), and median frequency of muscle fatigue index (MDF). All the data are expressed as mean ± standard deviation and are compared using a paired t-test. Athletes in shooting shoes scored higher than those in sports shoes (p < 0.05); COP moved less in the front-to-back and left-to-right directions for athletes wearing shooting shoes rather than sports shoes; and fewer muscles experienced fatigue, with fatigue also occurring later, for athletes wearing shooting shoes rather than sports shoes. Shooting shoes may reduce the sway of athletes’ center of pressure in the anterior–posterior and left–right directions, enhance postural stability, and result in higher shooting scores. In addition, they may make shooters feel more comfortable and relaxed during long training sessions and competitions.
Understanding the Meaningful Places for Aging-in-Place: A Human-Centric Approach toward Inter-Domain Design Criteria Consideration in Taiwan
Authors: Ling Tzen-Ying , Lu Hsien-Tsung , Kao Yen-Pin , Chien Szu-Cheng , Chen Hung-Chou , Lin Li-Fong
Published: January 2023

Background: Aging is key to inclusion, and it should be taken into account when designing every place of human activity. However, the implementation of such guidelines often fails the human-centric aspiration as health and design domain interpretation gaps impede the suitable reading and implementation strategies. Purpose: This study aimed to understand critical factors in the place-of-aging and to examine the gap in domain interpretation affecting age-friendly housing. Methods: Using grounded theory as a base, questionnaire interviews were implemented either face-to-face or through an online process by health and design domain experts. Overall, 40 respondents (20 health and 20 design experts) evaluated the key criteria to prioritize according to their value of importance. The factor analysis resulted in the stated deviation, suggesting a necessity to redefine the attributes of the dwelling based on a people, place and process framework. Results: The systemic analysis affirmed the inter-disciplinary gap to enhancing the dwelling provision. The health domain experts consistently ranked the criteria higher or equal than the design domain except for safety and security criteria. Both domains agreed that affordability is a main concern, as elders must be able to afford their dwelling choice. Conclusion: The valuable finding of the key criteria in the study is to uphold the value of the urban health resilience implication as the core of this study.
Using Metaphors to Understand Suffering in COVID-19 Survivors: A Two Time-Point Observational Follow-Up Study
Authors: Palese Alvisa , Visintini Erica , Bressan Valentina , Fonda Federico , Chiappinotto Stefania , Grassetti Luca , Peghin Maddalena , Tascini Carlo , Balestrieri Matteo , Colizzi Marco
Published: January 2023

Accumulating evidence indicates that the COVID-19 pandemic carries risks to psychological health and represents a collective traumatic experience with consequences at the social, economic, and health levels. The primary aim of this study was to collect ongoing COVID-19 survivors’ pandemic-related experiences as expressed through the use of metaphors; the secondary aim was to explore socio-demographic variables associated with the metaphor orientation as negative, positive or neutral. An observational follow-up survey was conducted and reported according to the STROBE guidelines. Patients ≥ 18 years, who were treated for COVID-19 during the first wave (March/April 2020) and who were willing to participate in a telephone interview were involved and asked to summarize their COVID-19 experience as lived up to 6 and 12 months in a metaphor. A total of 339 patients participated in the first (6 months) and second (12 months) data collection. Patients were mainly female (51.9%), with an average age of 52.9 years (confidence interval, CI 95% 51.2–54.6). At 6 months, most participants (214; 63.1%) used a negative-oriented metaphor, further increasing at 12 months (266; 78.5%), when they used fewer neutral-/positive-oriented metaphors (p < 0.001). At the 6-month follow-up, only three individual variables (female gender, education, and experiencing symptoms at the COVID-19 onset) were significantly different across the possible metaphor orientation; at 12 months, no individual variables were significantly associated. This study suggests increasingly negative lived experiences over time and the need for personalized healthcare pathways to face the long-term traumatic consequences of COVID-19.
Transcutaneous Auricular Vagus Nerve Stimulation Facilitates Cortical Arousal and Alertness
Authors: Chen Yuxin , Lu Xuejing , Hu Li
Published: January 2023

Transcutaneous auricular vagus nerve stimulation (taVNS) is a promising noninvasive technique with potential beneficial effects on human emotion and cognition, including cortical arousal and alertness. However, it remains unclear how taVNS could improve cortical arousal and alertness, which are crucial for consciousness and daily task performance. Here, we aimed to estimate the modulatory effect of taVNS on cortical arousal and alertness and to reveal its underlying neural mechanisms. Sixty subjects were recruited and randomly assigned to either the taVNS group (receiving taVNS for 20 min) or the control group (receiving taVNS for 30 s). The effects of taVNS were evaluated behaviorally using a cue-target pattern task, and neurologically using a resting-state electroencephalogram (EEG). We found that taVNS facilitated the reaction time for the targets requiring right-hand responses and attenuated high-frequency alpha oscillations under the close-eye resting state. Importantly, taVNS-modulated alpha oscillations were positively correlated with the facilitated target detection performance, i.e., reduced reaction time. Furthermore, microstate analysis of the resting-state EEG when the eyes were closed illustrated that taVNS reduced the mean duration of microstate C, which has been proven to be associated with alertness. Altogether, this work provided novel evidence suggesting that taVNS could be an enhancer of both cortical arousal and alertness.
Effect of Authigenic Chlorite on the Pore Structure of Tight Clastic Reservoir in Songliao Basin
Authors: Zhang Yangchen , Qu Xiyu , Miao Changsheng , Zhu Jianfeng , Xu Wen , Wang Weiming
Published: January 2023

Authigenic chlorite is a common clay mineral in clastic rock reservoirs, and it has an important influence on the pore structure of tight clastic rock reservoirs. In this paper, the tight clastic reservoirs in the Lower Cretaceous Yingcheng Formation in the Longfengshan subsag in the Changling fault depression in the Songliao Basin were investigated. Polarized light microscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), high-pressure mercury injection (HPMI), and low temperature nitrogen adsorption (LTNA) were used to study the influence of authigenic chlorite on the pore structure of tight clastic reservoirs. The results show that the authigenic chlorite in the study area was mainly generated in the form of pore linings. The formation of the authigenic chlorite was mainly controlled by the parent rock type and the sedimentary microfacies in the provenance area. The hydrolysis and dissolution of the iron- and magnesium-rich intermediate-mafic magmatic rocks and the high-energy, open, weakly alkaline reducing environment in the delta-front underwater distributary channel were the key factors controlling the formation of the authigenic chlorite in the study area. The pore-lining chlorite slowed down compaction and inhibited quartz overgrowth, protecting the original pores. Moreover, there are a large number of intercrystalline pores in the chlorite, which provided channels for the flow of acidic water and thus the formation of secondary pores, playing a positive role in the physical properties of the tight clastic rock reservoirs. However, the pore-filling chlorite also blocked the pore throats, playing a negative role in the physical properties of the tight clastic rock reservoirs. The tight clastic rock reservoirs with pore-lining chlorite generally had low displacement pressures and large pore throat radii. The morphology of the nano-scale pores was mainly parallel plate-shaped slit pores. There were many primary pores and a small number of secondary pores in the reservoir. Some of the pores were connected by narrow-necked or curved sheet-like throats, and the pore structure was relatively good. A higher relative content of chlorite led to a larger nano-scale pore throat radius, a smaller specific surface area, a smoother pore surface, and stronger homogeneity. Authigenic chlorite played a positive role in the formation of the tight clastic reservoirs in the study area.
Effect of Physical Activity/Exercise on Cardiorespiratory Fitness in Children and Adolescents with Type 1 Diabetes: A Scoping Review
Authors: Chang Xinyi , Wang Ziheng , Guo Hongzhi , Xu Yinghan , Ogihara Atsushi
Published: January 2023

The most common type of diabetes among children and adolescents is type 1 diabetes mellitus (T1DM), which is associated with an increased risk of cardiovascular disease (CVD). Additionally, lower levels of cardiorespiratory fitness (CRF) are linked to an increased risk of CVD. Regular exercise is associated with a decreased risk of CVD and improved CRF. We conducted this scoping review to assess the effects of exercise on CRF in youth with T1DM. Three electronic databases (PubMed, Embase, and Cochrane Central Register of Controlled Trials) were used to search for the relevant literature. In this analysis, the PICOS method was used to select studies and was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Guidelines scoping review guidelines for the evaluation of the effects of physical activity and cardiac function; the criteria may include the type and intensity of physical activity, the duration of the intervention, peak oxygen consumption (VO2), peak minute ventilation (VE), and peak heart rate of cardiorespiratory fitness. Screening resulted in 434 records. Of these, nine articles were included in our study. These nine studies were experimental (noncontrolled trials or randomized controlled trials) (n = 7) and observational (cross-sectional) (n = 2), and could be used to evaluate the effectiveness of physical activity interventions on cardiac function. The effects of exercise on CRF in youth with T1DM vary according to the type, frequency, and intensity of the exercise. According to our review, the duration of exercise included in the studies did not meet the recommendations of the guidelines for youth with T1DM. Additionally, half of the studies revealed that exercise could optimize the lipid profile in youth with T1DM. Hence, this research is to provide an overview of the effects of physical activity and exercise on CRF, cardiovascular fitness, lipid profile, and blood pressure in youth with T1DM, as well as identified potential limitations of the existing studies. Nevertheless, the limited number of clinical studies employing exercise interventions for children and adolescents with T1DM emphasize the need for more studies in this area, and more specific modes of exercise should be developed in the future.
Acknowledgment to the Reviewers of International Journal of Financial Studies in 2022
Authors:   International Journal of Financial Studies Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
Assessment of Neurotoxic Effects of Oxycodone and Naloxone in SH-SY5Y Cell Line
Authors: Lima Luíza Siqueira , da Costa Nayara de Souza , Galiciolli Maria Eduarda Andrade , Pereira Meire Ellen , Almeida William , Margarete Cestari Marta , Nogara Pablo Andrei , Irioda Ana Carolina , Oliveira Cláudia Sirlene
Published: January 2023

Opioid drugs have analgesic properties used to treat chronic and post-surgical pain due to descending pain modulation. The use of opioids is often associated with adverse effects or clinical issues. This study aimed to evaluate the toxicity of opioids by exposing the neuroblastoma cell line (SH-SY5Y) to 0, 1, 10, and 100 µM oxycodone and naloxone for 24 h. Analyses were carried out to evaluate cell cytotoxicity, identification of cell death, DNA damage, superoxide dismutase (SOD), glutathione S-transferase (GST), and acetylcholinesterase (AChE) activities, in addition to molecular docking. Oxycodone and naloxone exposure did not alter the SH-SY5Y cell viability. The exposure to 100 µM oxycodone and naloxone significantly increased the cells’ DNA damage score compared to the control group. Naloxone exposure significantly inhibited AChE, GST, and SOD activities, while oxycodone did not alter these enzymes’ activities. Molecular docking showed that naloxone and oxycodone interact with different amino acids in the studied enzymes, which may explain the differences in enzymatic inhibition. Naloxone altered the antioxidant defenses of SH-SY5Y cells, which may have caused DNA damage 24 h after the exposure. On the other hand, more studies are necessary to explain how oxycodone causes DNA damage.
The Overlooked Microbiome—Considering Archaea and Eukaryotes Using Multiplex Nanopore-16S-/18S-rDNA-Sequencing: A Technical Report Focusing on Nasopharyngeal Microbiomes
Authors: Baehren Carolin , Pembaur Anton , Weil Patrick P. , Wewers Nora , Schult Frank , Wirth Stefan , Postberg Jan , Aydin Malik
Published: January 2023

In contrast to bacteria, microbiome analyses often neglect archaea, but also eukaryotes. This is partly because they are difficult to culture due to their demanding growth requirements, or some even have to be classified as uncultured microorganisms. Consequently, little is known about the relevance of archaea in human health and diseases. Contemporary broad availability and spread of next generation sequencing techniques now enable a stronger focus on such microorganisms, whose cultivation is difficult. However, due to the enormous evolutionary distances between bacteria, archaea and eukaryotes, the implementation of sequencing strategies for smaller laboratory scales needs to be refined to achieve as a holistic view on the microbiome as possible. Here, we present a technical approach that enables simultaneous analyses of archaeal, bacterial and eukaryotic microbial communities to study their roles in development and courses of respiratory disorders. We thus applied combinatorial 16S-/18S-rDNA sequencing strategies for sequencing-library preparation. Considering the lower total microbiota density of airway surfaces, when compared with gut microbiota, we optimized the DNA purification workflow from nasopharyngeal swab specimens. As a result, we provide a protocol that allows the efficient combination of bacterial, archaeal, and eukaryotic libraries for nanopore-sequencing using Oxford Nanopore Technologies MinION devices and subsequent phylogenetic analyses. In a pilot study, this workflow allowed the identification of some environmental archaea, which were not correlated with airway microbial communities before. Moreover, we assessed the protocol’s broader applicability using a set of human stool samples. We conclude that the proposed protocol provides a versatile and adaptable tool for combinatorial studies on bacterial, archaeal, and eukaryotic microbiomes on a small laboratory scale.
Antibacterial and Anti-Inflammatory Properties of ZnO Nanoparticles Synthesized by a Green Method Using Sargassum Extracts
Authors: Lopez-Miranda Jose Luis , Molina Gustavo A. , González-Reyna Marlen Alexis , España-Sánchez Beatriz Liliana , Esparza Rodrigo , Silva Rodolfo , Estévez Miriam
Published: January 2023

The present work shows the synthesis of ZnO nanoparticles through a green method, using sargassum extracts, which provide the reducing and stabilizing compounds. The conditions of the medium in which the reaction was carried out was evaluated, that is, magnetic stirring, ultrasound assisted, and resting condition. UV-Vis, FTIR spectroscopy, and X-ray diffraction results confirmed the synthesis of ZnO with nanometric crystal size. The scanning electron microscopy analysis showed that the morphology and size of the particles depends on the synthesis condition used. It obtained particles between 20 and 200 nm in the sample without agitation, while the samples with stirring and ultrasound were 80 nm and 100 nm, respectively. ZnO nanoparticles showed antibacterial activity against Gram-positive S. aureus and Gram-negative P. aeruginosa. A quantitative analysis was performed by varying the concentration of ZnO nanoparticles. In all cases, the antibacterial activity against Gram-positives was greater than against Gram-negatives. Ultrasound-assisted ZnO nanoparticles showed the highest activity, around 99% and 80% for S. aureus and P. aeruginosa, respectively. Similar results were obtained in the study of the anti-inflammatory activity of ZnO nanoparticles; the ultrasound-assisted sample exhibited the highest percentage (93%), even above that shown by diclofenac, which was used as a reference. Therefore, the ZnO nanoparticles synthesized with sargassum extracts have properties that can be used safely and efficiently in the field of biomedicine.
Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation
Authors: Textor Martin , Hoburg Arnd , Lehnigk Rex , Perka Carsten , Duda Georg N. , Reinke Simon , Blankenstein Antje , Hochmann Sarah , Stockinger Andreas , Resch Herbert , Wolf Martin , Strunk Dirk , Geissler Sven
Published: January 2023

Loose bodies (LBs) from patients with osteochondritis dissecans (OCD) are usually removed and discarded during surgical treatment of the defect. In this study, we address the question of whether these LBs contain sufficient viable and functional chondrocytes that could serve as a source for autologous chondrocyte implantation (ACI) and how the required prolonged in vitro expansion affects their phenotype. Chondrocytes were isolated from LBs of 18 patients and compared with control chondrocyte from non-weight-bearing joint regions (n = 7) and bone marrow mesenchymal stromal cells (BMSCs, n = 6) obtained during primary arthroplasty. No significant differences in the initial cell yield per isolation and the expression of the chondrocyte progenitor cell markers CD44 + /CD146+ were found between chondrocyte populations from LBs (LB-CH) and control patients (Ctrl-CH). During long-term expansion, LB-CH exhibited comparable viability and proliferation rates to control cells and no ultimate cell cycle arrest was observed within 12 passages respectively 15.3 ± 1.1 mean cumulative populations doublings (CPD). The chondrogenic differentiation potential was comparable between LB-CH and Ctrl-CH, but both groups showed a significantly higher ability to form a hyaline cartilage matrix in vitro than BMSC. Our data suggest that LBs are a promising cell source for obtaining qualitatively and quantitatively suitable chondrocytes for therapeutic applications, thereby circumventing donor site morbidity as a consequence of the biopsies required for the current ACI procedure.
Peculiar Ca2+ Homeostasis, ER Stress, Autophagy, and TG2 Modulation in Celiac Disease Patient-Derived Cells
Authors: Sposito Silvia , Secondo Agnese , Romanelli Antonio Massimiliano , Montefusco Antonio , Nanayakkara Merlin , Auricchio Salvatore , Barone Maria Vittoria , Caputo Ivana , Paolella Gaetana
Published: January 2023

Celiac disease (CD) is an inflammatory intestinal disease caused by the ingestion of gluten-containing cereals by genetically predisposed individuals. Constitutive differences between cells from CD patients and control subjects, including levels of protein phosphorylation, alterations of vesicular trafficking, and regulation of type 2 transglutaminase (TG2), have been reported. In the present work, we investigated how skin-derived fibroblasts from CD and control subjects responded to thapsigargin, an endoplasmic reticulum ER stress inducer, in an attempt to contribute to the comprehension of molecular features of the CD cellular phenotype. We analyzed Ca2+ levels by single-cell video-imaging and TG2 activity by a microplate assay. Western blots and PCR analyses were employed to monitor TG2 levels and markers of ER stress and autophagy. We found that the cytosolic and ER Ca2+ level of CD cells was lower than in control cells. Treatments with thapsigargin differently activated TG2 in control and CD cells, as well as caused slightly different responses regarding the activation of ER stress and the expression of autophagic markers. On the whole, our findings identified further molecular features of the celiac cellular phenotype and highlighted that CD cells appeared less capable of adapting to a stress condition and responding in a physiological way.
Curcumin Reduces Pathological Endoplasmic Reticulum Stress through Increasing Proteolysis of Mutant Matrilin-3
Authors: Dennis Ella P. , Watson Robyn N. , McPate Florence , Briggs Michael D.
Published: January 2023

The intracellular retention of mutant cartilage matrix proteins and pathological endoplasmic reticulum (ER) stress disrupts ossification and has been identified as a shared disease mechanism in a range of skeletal dysplasias including short limbed-dwarfism, multiple epiphyseal dysplasia type 5 (EDM5). Although targeting ER stress is an attractive avenue for treatment and has proven successful in the treatment of a related skeletal dysplasia, to date no drugs have proven successful in reducing ER stress in EDM5 caused by the retention of mutant matrilin-3. Our exciting findings show that by using our established luciferase ER stress screening assay, we can identify a “natural” chemical, curcumin, which is able to reduce pathological ER stress in a cell model of EDM5 by promoting the proteasomal degradation mutant matrilin-3. Therefore, this is an important in vitro study in which we describe, for the first time, the success of a naturally occurring chemical as a potential treatment for this currently incurable rare skeletal disease. As studies show that curcumin can be used as a potential treatment for range of diseases in vitro, current research is focused on developing novel delivery strategies to enhance its bioavailability. This is an important and exciting area of research that will have significant clinical impact on a range of human diseases including the rare skeletal disease, EDM5.
Synthesis and Applications of Halide Perovskite Nanocrystals in Optoelectronics
Authors: Wang Yen Po , Li Hsin Chieh , Huang Yan Chi , Tan Chih Shan
Published: January 2023

The perovskites used for optoelectronic devices have been more attractive during recent years due to their wide variety of advantages, such as their low cost, high photoluminescence quantum yield (PLQY), high carrier mobility, flexible bandgap tunability, and high light absorption ability. However, optoelectronic applications for traditional inorganic and organic materials present dilemmas due to their hardly tunable bandgap and instability. On the other hand, there are some more important benefits for perovskite nanocrystals, such as a size-dependent bandgap and the availability of anion exchange at room temperature. Therefore, perovskite NC-based applications are currently favored, offering a research direction beyond perovskite, and much research has focused on the stability issue and device performance. Thus, the synthesis and applications of perovskite NCs need to be thoroughly discussed for the future development of solar cells, light-emitting diodes, photodetectors, and laser research.
Low-Cost Inventions and Patents: Series II
Authors: Salmerón-Manzano Esther , Manzano-Agugliaro Francisco
Published: January 2023

Inventions are new, unique, and useful creations or solutions to problems that have been developed through the process of invention [...]
Linking Error in the 2PL Model
Authors: Robitzsch Alexander
Published: January 2023

The two-parameter logistic (2PL) item response model is likely the most frequently applied item response model for analyzing dichotomous data. Linking errors quantify the variability in means or standard deviations due to the choice of items. Previous research presented analytical work for linking errors in the one-parameter logistic model. In this article, we present linking errors for the 2PL model using the general theory of M-estimation. Linking errors are derived in the case of log-mean-mean linking for linking two groups. The performance of the newly proposed formulas is evaluated in a simulation study. Furthermore, the linking error estimation in the 2PL model is also treated in more complex settings, such as chain linking, trend estimation, fixed item parameter calibration, and concurrent calibration.
A Systems Biology Approach for Addressing Cisplatin Resistance in Non-Small Cell Lung Cancer
Authors: Ramisetty Sravani , Kulkarni Prakash , Bhattacharya Supriyo , Nam Arin , Singhal Sharad S. , Guo Linlin , Mirzapoiazova Tamara , Mambetsariev Bolot , Mittan Sandeep , Malhotra Jyoti , Pisick Evan , Subbiah Shanmuga , Rajurkar Swapnil , Massarelli Erminia , Salgia Ravi , Mohanty Atish
Published: January 2023

Translational research in medicine, defined as the transfer of knowledge and discovery from the basic sciences to the clinic, is typically achieved through interactions between members across scientific disciplines to overcome the traditional silos within the community. Thus, translational medicine underscores ‘Team Medicine’, the partnership between basic science researchers and clinicians focused on addressing a specific goal in medicine. Here, we highlight this concept from a City of Hope perspective. Using cisplatin resistance in non-small cell lung cancer (NSCLC) as a paradigm, we describe how basic research scientists, clinical research scientists, and medical oncologists, in true ‘Team Science’ spirit, addressed cisplatin resistance in NSCLC and identified a previously approved compound that is able to alleviate cisplatin resistance in NSCLC. Furthermore, we discuss how a ‘Team Medicine’ approach can help to elucidate the mechanisms of innate and acquired resistance in NSCLC and develop alternative strategies to overcome drug resistance.
Improved Progression-Free Survival Associated with Tumor-Infiltrating Lymphocytes in High-Grade Endometrial Cancer
Authors: Fan Chun-Ting , Hsu Shih-Tien , Sun Lou , Hwang Sheau-Feng , Liu Chih-Ku , Shih Yu-Hsiang , Chen Ming-Jer , Li Hsin-Ni , Wang Jun-Sing , Wen Mei-Chin , Lu Chien-Hsing
Published: January 2023

Tumor-infiltrating lymphocytes (TILs) have emerged as a prognostic marker in endometrial cancer (EC). However, the role of TILs in EC with distinct histology grades and molecular types (such as mismatch repair [MMR] deficiency) has not yet been made clear. We retrospectively included 237 patients with primary EC who underwent a standard staging operation of laparoscopic or laparotomy total hysterectomy and bilateral salpingo-oophorectomy for analyses. An independent pathologist who was blind to the study patients’ information reviewed the pathologic slides to assess TILs according to the method introduced by the International Immuno-Oncology Biomarkers Working Group in 2017. The outcomes of interest included both progression-free survival (PFS) and overall survival (OS). The Kaplan–Meier method was used to determine the curves of PFS and OS according to TILs, and also in the relevant subgroups (low-grade vs. high-grade, MMR-proficient vs. MMR-deficient). After a median follow-up duration of 1.82 years, 18 patients had experienced either disease progression or death. Overall, TILs (+) were not associated with PFS or OS. We did observe, however, that TILs (+) were associated with a better PFS (p = 0.045) in patients with high-grade EC, but not in those with low-grade tumors (p = 0.733). The effect of TILs on PFS was not observed in patients with MMR-proficient (p = 0.347) or MMR-deficient (p = 0.168) EC. TILs were associated with a better PFS in patients with high-grade EC. Our results suggest that TILs may be a potential prognostic marker in these patients.
Changes in Choroidal Thickness and Structure in Preeclampsia with Serous Retinal Detachment
Authors: Fukui Ayumi , Tanaka Hiroshi , Terao Nobuhiro , Nagata Kenji , Matsumoto Akifumi , Kusada Natsuki , Kojima Kentaro , Sotozono Chie
Published: January 2023

Preeclampsia is a pregnancy-specific syndrome characterized by hypertension and proteinuria. We retrospectively investigated the clinical features, including choroidal layer thickness and luminal area to stromal area ratio, in a case series of preeclampsia with serous retinal detachment (SRD). The subjects were pregnant women with SRD during hospitalization for preeclampsia from October 2014 to June 2021. Based on medical records, affected eyes, time of onset, fundus examination findings, and subfoveal choroidal thickness (SCT), the choroidal layer thickness and choroidal vascular index (CVI) in each patient was examined. Thirteen eyes from seven patients (mean age 30.7 ± 4.7 years) were included in the study. In all cases, SRD improved without topical ocular treatment. The mean SCT at the initial visit was 424.4 ± 70.5 μm, and all patients had choroidal thickening, which significantly decreased to 286.0 ± 57.9 μm (p < 0.01) at the last visit. The mean choroidal inner layer was 162.7 ± 69.4 μm at the initial visit and 122.3 ± 35.5 μm at the final follow-up visit (p = 0.06), showing no significant difference; however, the mean choroidal outer layer was 261.7 ± 47.6 μm at the initial visit and 163.7 ± 37.1 μm at the final follow-up visit (p < 0.01), thus showing a significant decrease. The mean CVI was 67.2 ± 1.3% at the initial visit, yet it had significantly decreased to 65.4 ± 1.1% (p < 0.01) at the final follow-up visit. The findings of this study show that SRD with preeclampsia is associated with increased thickening of the choroidal outer layer, especially in the choroidal luminal area.