bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination
Authors: Berbers Bas , Ceyssens Pieter-Jan , Bogaerts Pierre , Vanneste Kevin , Roosens Nancy H. C. , Marchal Kathleen , De Keersmaecker Sigrid C. J.
Published: August 2020

Antimicrobial resistance (AMR) is one of the most prominent public health threats. AMR genes localized on plasmids can be easily transferred between bacterial isolates by horizontal gene transfer, thereby contributing to the spread of AMR. Next-generation sequencing (NGS) technologies are ideal for the detection of AMR genes; however, reliable reconstruction of plasmids is still a challenge due to large repetitive regions. This study proposes a workflow to reconstruct plasmids with NGS data in view of AMR gene localization, i.e., chromosomal or on a plasmid. Whole-genome and plasmid DNA extraction methods were compared, as were assemblies consisting of short reads (Illumina MiSeq), long reads (Oxford Nanopore Technologies) and a combination of both (hybrid). Furthermore, the added value of conjugation of a plasmid to a known host was evaluated. As a case study, an isolate harboring a large, low-copy mcr-1-carrying plasmid (>200 kb) was used. Hybrid assemblies of NGS data obtained from whole-genome DNA extractions of the original isolates resulted in the most complete reconstruction of plasmids. The optimal workflow was successfully applied to multidrug-resistant Salmonella Kentucky isolates, where the transfer of an ESBL-gene-containing fragment from a plasmid to the chromosome was detected. This study highlights a strategy including wet and dry lab parameters that allows accurate plasmid reconstruction, which will contribute to an improved monitoring of circulating plasmids and the assessment of their risk of transfer.
Batch and Flow Ultrasound-Assisted Extraction of Grape Stalks: Process Intensification Design up to a Multi-Kilo Scale
Authors: Grillo Giorgio , Boffa Luisa , Talarico Salvatore , Solarino Roberto , Binello Arianna , Cavaglià Giuliano , Bensaid Samir , Telysheva Galina , Cravotto Giancarlo
Published: August 2020

Nowadays, approximately 1 billion kg/y of grape stalks, with a remarkable polyphenols content, are produced worldwide. In this paper, the extraction process intensification of polyphenols in water was achieved under ultrasound-assisted recovery, focusing on kinetics and scaling-up factors. Immersion and cup-horn systems were exploited as acoustic cavitation sources, and the total phenolic content (TPC) was chosen to assess the process efficiency. The kinetics were evaluated by Peleg’s hyperbolic model, and the effect of both the initial feedstock granulometry and ultrasound size-reduction were determined. The results were compared with conventional extraction methods (data analysis by ANOVA). The best polyphenols yield was obtained after 45 min of sonication, giving between 29.71 and 31.89 mg/g (gallic acid equivalents over the dry matter). The extracts were characterized using HPLC-DAD, UPLC-ESI-MS/MS, DPPH• assay (2,2-diphenyl-1-picrylhydrazyl), TEAC assay (Trolox equivalent antioxidant capacity), and proanthocyanidin content determination. The flow-mode extraction procedure of grape stalks (2 kg) was carried out in a 15 L reactor. A semi-industrial decanter unit and a bag-filter were the keys units of the downstream operations. The resulting particle-free solution underwent nanofiltration on a membrane pilot skid, providing a final polyphenols-enriched stream concentrated up to 355.91%, as shown by the antioxidant activity and TPC.
Integrated Hypoxia Signaling and Oxidative Stress in Developmental Neurotoxicity of Benzo[a]Pyrene in Zebrafish Embryos
Authors: Lin Yi-Chen , Wu Chang-Yi , Hu Chin-Hwa , Pai Tun-Wen , Chen Yet-Ran , Wang Wen-Der
Published: August 2020

Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon formed by the incomplete combustion of organic matter. Environmental B[a]P contamination poses a serious health risk to many organisms because the pollutant may negatively affect many physiological systems. As such, chronic exposure to B[a]P is known to lead to locomotor dysfunction and neurodegeneration in several organisms. In this study, we used the zebrafish model to delineate the acute toxic effects of B[a]P on the developing nervous system. We found that embryonic exposure of B[a]P downregulates shh and isl1, causing morphological hypoplasia in the telencephalon, ventral thalamus, hypothalamus, epiphysis and posterior commissure. Moreover, hypoxia-inducible factors (hif1a and hif2a) are repressed upon embryonic exposure of B[a]P, leading to reduced expression of the Hif-target genes, epo and survivin, which are associated with neural differentiation and maintenance. During normal embryogenesis, low-level oxidative stress regulates neuronal development and function. However, our experiments revealed that embryonic oxidative stress is greatly increased in B[a]P-treated embryos. The expression of catalase was decreased and sod1 expression increased in B[a]P-treated embryos. These transcriptional changes were coincident with increased embryonic levels of H2O2 and malondialdehyde, with the levels in B[a]P-treated fish similar to those in embryos treated with 120-μM H2O2. Together, our data suggest that reduced Hif signaling and increased oxidative stress are involved in B[a]P-induced acute neurotoxicity during embryogenesis.
Comparison of In Vitro and In Vivo Antioxidant Activities of Six Flavonoids with Similar Structures
Authors: Zeng Yixiu , Song Jiajia , Zhang Meimei , Wang Hongwei , Zhang Yu , Suo Huayi
Published: August 2020

The in vitro and in vivo antioxidant activities of six flavonoids with similar structures, including epicatechin (EC), epigallocatechin (EGC), procyanidin B2 (P), quercetin (Q), taxifolin (T), and rutin (R) were compared. The structures of the six flavonoids and their scavenging activities for 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radicals were closely related. The flavonoids decreased serum contents of malondialdehyde (MDA) and nitric oxide (NO), and increased serum total antioxidative capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels to different degrees in d-galactose-treated mice. The changes in mRNA expression of liver GSH-Px1, CAT, SOD1, and SOD2 by d-galactose were dissimilarly restored by the six flavonoids. Moreover, the six flavonoids differentially prevented the inflammatory response caused by oxidative stress by inhibiting interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α levels, and restoring IL-10 levels. These six flavonoids from two subclasses revealed the following antioxidant capability: P > EC, EGC > EC, Q > T, Q > R. Our results indicate that (1) the pyrogallol, dimerization, and C2=C3 double bonds of flavonoids enhanced antioxidant activity and (2) the C3 glycosylation of flavonoids attenuated antioxidant capacity.
Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice
Authors: Leyva-Jiménez Francisco Javier , Ruiz-Malagón Antonio Jesús , Molina-Tijeras José Alberto , Diez-Echave Patricia , Vezza Teresa , Hidalgo-García Laura , Lozano-Sánchez Jesús , Arráez-Román David , Cenis José Luis , Lozano-Pérez Antonio Abel , Rodríguez-Nogales Alba , Segura-Carretero Antonio , Gálvez Julio
Published: August 2020

Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH• radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.
Effect of Adding Curcumin on the Properties of Linseed Oil Organogels Used as Fat Replacers in Pâtés
Authors: Ramírez-Carrasco Patricia , Paredes-Toledo Javier , Romero-Hasler Patricio , Soto-Bustamante Eduardo , Díaz-Calderón Paulo , Robert Paz , Giménez Begoña
Published: August 2020

Beeswax-based organogels were formulated with linseed oil and curcumin according to a statistical design to increase the oxidative stability of spreadable meat products (pâté) where these organogels (OGCur) were incorporated as fat substitutes. The organogels obtained under optimal conditions (9.12% beeswax, 0.54% curcumin) showed a mechanical strength similar to pork backfat determined by back extrusion and high oil binding capacity (OBC; over 90%). The incorporation of curcumin at this concentration did not lead to any change in the arrangement of the crystal network, OBC, and mechanical, thermal, or rheological properties of the organogels. Beeswax organogels with and without curcumin, with a β’ orthorhombic subcell structure, showed a predominant elastic behavior and a melting event wider and shifted to lower temperatures than pure beeswax, suggesting a plasticizer effect of the oil in the wax crystals. The oxidative stability of the organogels under accelerated oxidation conditions increased due to the incorporation of curcumin. A decrease in the curcumin content was found from day 4 at 60 °C, together with a significantly lower formation of both peroxides and malonaldehyde. When pork backfat was partially or totally replaced by OGCur in pâtés, a noticeable protective effect of curcumin against lipid oxidation was found during chilled storage
Conventional Soliton and Noise-Like Pulse Generated in an Er-Doped Fiber Laser with Carbon Nanotube Saturable Absorbers
Authors: Dong Zikai , Tian Jinrong , Li Runlai , Cui Youshuo , Zhang Wenhai , Song Yanrong
Published: August 2020

Conventional soliton (CS) and noise-like pulse (NLP) are two different kinds of pulse regimes in ultrafast fiber lasers, which have many intense applications. In this article, we experimentally demonstrate that the pulse regime of an Er-doped fiber laser could be converted between conventional soliton and noise-like pulse by using fast response saturable absorbers (SA) made from different layers of single-wall carbon nanotubes (CNT). For the monolayer (ML) single-wall CNT-SA, CS with pulse duration of 439 fs at 1560 nm is achieved while for the bilayer (BL) single-wall CNT, NLP at 1560 nm with a 1.75 ps spike and a 98 ps pedestal is obtained. The transition mechanism from CS to NLP is investigated by analyzing the optical characteristics of ML and BL single-wall CNT. The further theoretical simulation illustrates that CNT-SA enables the switching between CS and NLP in anomalous dispersion regime in Er-doped fiber lasers.
Sound Effects on Standing Postural Strategies in the Elderly via Frequency Analysis Approach
Authors: Lee Yun-Ju , Chen Chang-Hsu , Wu Chao-Che , Chen Yu-Jung , Liang Jing Nong
Published: August 2020

Sound and sound frequency could improve postural sway in the elderly. The power spectrum intervals of the center of pressure (COP) displacement are associated with different postural regulations, which could be revealed by frequency analysis. The aim of the study was to investigate the effects of sound on dual-tasking postural control and conduct frequency analysis to distinguish postural regulations in the elderly. Fifteen young and 15 older healthy participants were instructed to stand on a force platform and performed the Purdue Pegboard test while hearing 50 dB sounds with sound frequencies of 250 Hz, 1000 Hz, 4000 Hz, or no sound. The total excursion, velocity, sway area, and power spectrum of low-, medium-, and high-frequency bands of the COP displacement were calculated in the anterior–posterior and medial–lateral directions. The percentages of low-frequency and medium-frequency bands in both directions were significantly different between with and without sound conditions, but not affected by sound frequency. Older adults showed a smaller percentage of low-frequency, larger percentage of medium-frequency, larger total COP excursion, and faster velocity in the medial–lateral direction. The outcome of the study supports the frequency analysis approach in evaluating sound effects on postural strategies in dual-tasking and reveals older adults utilize vestibular regulation as the primary postural strategy when the dual-task required visual attention.
Soil Moisture Analysis by Means of Multispectral Images According to Land Use and Spatial Resolution on Andosols in the Colombian Andes
Authors: Casamitjana Maria , Torres-Madroñero Maria C. , Bernal-Riobo Jaime , Varga Diego
Published: August 2020

Surface soil moisture is an important hydrological parameter in agricultural areas. Periodic measurements in tropical mountain environments are poorly representative of larger areas, while satellite resolution is too coarse to be effective in these topographically varied landscapes, making spatial resolution an important parameter to consider. The Las Palmas catchment area near Medellin in Colombia is a vital water reservoir that stores considerable amounts of water in its andosol. In this tropical Andean setting, we use an unmanned aerial vehicle (UAV) with multispectral (visible, near infrared) sensors to determine the correlation of three agricultural land uses (potatoes, bare soil, and pasture) with surface soil moisture. Four vegetation indices (the perpendicular drought index, PDI; the normalized difference vegetation index, NDVI; the normalized difference water index, NDWI, and the soil-adjusted vegetation index, SAVI) were applied to UAV imagery and a 3 m resolution to estimate surface soil moisture through calibration with in situ field measurements. The results showed that on bare soil, the indices that best fit the soil moisture results are NDVI, NDWI and PDI on a detailed scale, whereas on potatoes crops, the NDWI is the index that correlates significantly with soil moisture, irrespective of the scale. Multispectral images and vegetation indices provide good soil moisture understanding in tropical mountain environments, with 3 m remote sensing images which are shown to be a good alternative to soil moisture analysis on pastures using the NDVI and UAV images for bare soil and potatoes.
Drying Effect on Enzymatic Hydrolysis of Cellulose Associated with Porosity and Crystallinity
Authors: Koo Bonwook , Jo Jaemin , Cho Seong-Min
Published: August 2020

The effect of drying on the enzymatic hydrolysis of cellulose was determined by analysis of porosity and crystallinity. Fiber hornification induced by drying produced an irreversible reduction in pore volume due to shrinkage and pore collapse, and the decrease in porosity inhibited enzymatic hydrolysis. The drying effect index (DEI) was defined as the difference in enzymatic digestibility between oven- and never-dried pulp, and it was determined that more enzymes caused a higher DEI at the initial stage of enzymatic hydrolysis and the highest DEI was also observed at the earlier stages with higher enzyme dosage. However, there was no significant difference in the DEI with less enzymes because cellulose conversion to sugars during hydrolysis did not enhance enzymatic hydrolysis due to the decrease in enzyme activity. The water retention value (WRV) and Simons’ staining were used to measure pore volume and to investigate the cause of the decrease in enzymatic hydrolysis. A decrease in enzyme accessibility induced by the collapse of enzymes’ accessible larger pores was determined and this decreased the enzymatic hydrolysis. However, drying once did not cause any irreversible change in the crystalline structure, thus it seems there is no correlation between enzymatic digestibility and crystalline structure.
An Incentive Factor-Based Dynamic Comprehensive Evaluation on a High-Speed Railway Track
Authors: Wang Xiaohui , Yang Jianwei , Wang Jinhai , Wang Yanxue , Xu Guiyang
Published: August 2020

Peak management and mean management are common ways to manage the quality of high-speed railway tracks at present. The most popular method for evaluating such tracks is the track quality index (TQI) method, which can reflect the overall state of the equipment to a certain extent. However, this method is likely to ignore some potential risks that threaten the operation of a high-speed train. For more effective risk identification, an incentive factor-based dynamic comprehensive evaluation (DCE) method was introduced to assess the geometric parameters of a high-speed railway track. Moreover, the weights of geometric parameters were computed by a combination of the analytic hierarchy process (AHP) and entropy based on the correlation coefficient. The proposed method can highlight the sensitivity index of the geometric parameters, which is an advantage over the TQI method. A case study of a high-speed railway track was performed using the two methods, and the results were verified with the original data. It was found that the TQI method identified only one obvious risk while the proposed method identified one obvious risk and two potential risks. This suggests that the proposed method is more accurate in identifying the risky sections than the TQI method.
Revisiting the Sodiation Mechanism of TiO2 via Operando X-ray Absorption Spectroscopy
Authors: Fehse Marcus , Henry Aurélien , Zitolo Andrea , Boury Bruno , Louvain Nicolas , Stievano Lorenzo
Published: August 2020

The sodiation mechanism of TiO2 anatase was thoroughly investigated via X-ray absorption spectroscopy under operando conditions. The data set was analysed via an innovative and smart approach based on chemometric tools that allows the unbiased and reliable extraction of the maximum amount of meaningful information. The resulting data analysis reveals that the electrochemical sodiation mechanism is mainly based on the reduction of Ti4+ to Ti3+, going along with the irreversible amorphisation of the pristine anatase structure. At least one semi-amorphous intermediate is formed during the first discharge, whose local structure resembles those obtained at the end of the charge.
Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticus
Authors: Maetani-Yasui Miki , Mawatari Kazuaki , Honjo Airi , Bui Thi Kim Ngan , Shimohata Takaaki , Uebanso Takashi , Aihara Mutsumi , Emoto Takahiro , Akutagawa Masatake , Kinouchi Yohsuke , Takahashi Akira
Published: August 2020

Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.
Quantum Turing Machines: Computations and Measurements
Authors: Guerrini Stefano , Martini Simone , Masini Andrea
Published: August 2020

Contrary to the classical case, the relation between quantum programming languages and quantum Turing Machines (QTM) has not been fully investigated. In particular, there are features of QTMs that have not been exploited, a notable example being the intrinsic infinite nature of any quantum computation. In this paper, we propose a definition of QTM, which extends and unifies the notions of Deutsch and Bernstein & Vazirani. In particular, we allow both arbitrary quantum input, and meaningful superpositions of computations, where some of them are “terminated” with an “output”, while others are not. For some infinite computations an “output” is obtained as a limit of finite portions of the computation. We propose a natural and robust observation protocol for our QTMs, which does not modify the probability of the possible outcomes of the machines. Finally, we use QTMs to define a class of quantum computable functions—any such function is a mapping from a general quantum state to a probability distribution of natural numbers. We expect that our class of functions, when restricted to classical input-output, will not be different from the set of the recursive functions.
Numerical Analysis of the Detailed Balance of Multiple Exciton Generation Solar Cells with Nonradiative Recombination
Authors: Lee Jongwon , Honsberg Christiana B.
Published: August 2020

In this study, we analyzed the nonradiative recombination impact of multiple exciton generation solar cells (MEGSCs) with a revised detailed balance (DB) limit. The nonideal quantum yield (QY) of a material depends on the surface defects or the status of the material. Thus, its QY shape deviates from the ideal QY because of carrier losses. We used the ideal reverse saturation current variation in the DB of MEGSCs to explain the impact of nonradiative recombination. We compared ideal and nonideal QYs with the nonradiative recombination into the DB of MEGSCs under one-sun and full-light concentration. Through this research, we seek to develop a strategy to maintain MEGSC performance.
Modeling of Dental Implant Osseointegration Progress by Three-Dimensional Finite Element Method
Authors: Roatesi Iulia , Roatesi Simona
Published: August 2020

As osseointegration is a time-dependent process, biomechanical assessment is thought to determine whether a fibrous encapsulation or a bone covering will develop around an implant, according to the stress in the implant and surrounding bone. This study proposes a model for stress evaluation by finite element method (FEM) during the osseointegration progress, the main factor implied in implant success or failure. The loadings due to masticatory forces generate stress concentration and consequently, an adequate risk concerning the implant stability should be assessed. An accurate FEM model is used to calculate the stress and displacement in the whole implant–bone system during the osseointegration progress. This process is simulated by taking into account the gradual increase in the damaged biomechanical properties of the cortical bone. The results reveal that as the implant osseointegration occurs gradually, the bone stiffness from the peri-implant area increases gradually, such that in the end (healing) we observed that the cortical bone begins to take over the bending loading. In addition, the displacements decrease as the osseointegration gradually occurs and the cortical bone stress reaches higher values, which are placed in the mandibular ridge. The FEM is suitable to model the osseointegration progress, offering valuable information concerning the stress concentration zones in the implant–bone system and consequently, the risk evaluation, both for pre- and post-osseointegration.
Abrasion Behavior of Steel-Fiber-Reinforced Concrete in Hydraulic Structures
Authors: Liu Yu-Wen , Lin Yu-Yuan , Cho Shih-Wei
Published: August 2020

This study investigated two types of abrasion resistance of steel–fiber-reinforced concrete in hydraulic structures, friction abrasion and impact abrasion using the ASTM C1138 underwater test and the water-borne sand test, respectively. Three water-to-cementitious-material ratios (0.50, 0.36, and 0.28), two impact angles (45° and 90°), plain concrete, and steel–fiber-reinforced concrete were employed. Test results showed that the abrasive action and principal resistance varied between the two test methods. The average impact abrasion rates (IARs) of concrete were approximately 8–17 times greater than the average friction abrasion rate (FARs). In general, the impact abrasion loss of the concrete surface impacted at a vertical angle was higher than that of impacted at a 45 degree angle. Moreover, the average FAR and IAR decreased when the concrete was reinforced with steel fibers. The steel fibers acted as shields to prevent the concrete material behind the fibers from abrasion, thus improving abrasion resistance. In both the underwater and waterborne sand flow methods, the resistance to abrasion of concrete without steel fibers increased as the water/cementitious material ratio (w/cm) decreased, and the concrete compressive strength also increased.
Adaptability Evaluation of Metal-Ceramic Crowns Obtained by Additive and Subtractive Technologies
Authors: Savencu Cristina Elena , Șerban Costela , Porojan Liliana
Published: August 2020

(1) Background: Traditional metal-ceramic restorations are considered as a standard in the evaluation of new technologies. A critical factor in their longevity is represented by their adaptability; The purpose of this study was to evaluate the marginal and internal gap of ceramic-fused to metal crowns with frameworks obtained by additive manufacturing (AM) technologies and the influence of veneering process on their fit; (2) Methods: Metal-ceramic crowns have been produced by conventional lost-wax technology (T), digital milling (F), selective laser sintering (SLS) and selective laser melting (SLM). The adaptability was assessed using silicone replicas before and after ceramic veneering; (3) Results: The best values were obtained for the milled group followed closely by SLM and SLS, and a significantly higher gap for casted copings. The veneering process did not significantly influence the adaptability of the crowns, regardless of the manufacturing process used for frameworks. The present study promotes additive technologies (AT) as a fast, efficient, and cost-effective alternative to traditional technology. There are fewer steps in which errors can occur when digital technologies are used and the risk of distortion is diminished. (4) Conclusions: CAD/CAM technologies, both additive and subtractive, represent an excellent option to produce time-effective, precise metal-ceramic crowns with excellent adaptation.
Single-Parameter-Tuned Attitude Control for Quadrotor with Unknown Disturbance
Authors: Hu Dada , Pei Zhongcai , Tang Zhiyong
Published: August 2020

In this paper, methods are presented for designing a quadrotor attitude control system with disturbance rejection ability, wherein only one parameter needs to be tuned for each axis. The core difference between quadrotor platforms are extracted as critical gain parameters (CGPs). Reinforcement learning (RL) technology is introduced in order to automatically optimize the controlling law for quadrotors with different CGPs, and the CGPs are used to extend the RL state list. A deterministic policy gradient (DPG) algorithm that is based on an actor-critic structure in a model-free style is used as the learning algorithm. Mirror sampling and reward shaping methods are designed in order to eliminate the steady-state errors of the RL controller and accelerate the training process. Active disturbance rejection control (ADRC) is applied to reject unknown external disturbances. A set of extended state observers (ESOs) is designed to estimate the total disturbance to the roll and pitch axes. The covariance matrix adaptation evolution strategy (CMA-ES) algorithm is used to automatically tune the ESO parameters and improve the final performance. The complete controller is tested on an F550 quadrotor in both simulation and real flight environments. The quadrotor can hover and move around stably and accurately in the air, even with a severe disturbance.
Effects of Lasers and Their Delivery Characteristics on Machined and Micro-Roughened Titanium Dental Implant Surfaces
Authors: Fenelon Thomas , Bakr Mahmoud M. , Walsh Laurence J. , George Roy
Published: August 2020

The aim of the study was to investigate the effects of neodymium: yttrium aluminium garnet (Nd:YAG) (1064 nm) and erbium: yttrium aluminium garnet (Er:YAG) (2940 nm) laser energy on titanium when delivered with conventional optics (focusing handpieces or plain ended optical fibres) or with a conical tip. Machined and micro-roughened implant discs were subjected to laser irradiation under a variety of energy settings either dry (without water) or wet (with water). Samples were scanned using a 3D non-contact laser profilometer and analysed for surface roughness, volume of peaks and the maximum diameter of the ablated area. Conical tip designs when used with both lasers showed no surface effect at any power setting on both machined and micro-roughened implant surfaces, regardless of the irrigation condition. When used with conventional delivery systems, laser effects on titanium were dose related, and were more profound with the Nd:YAG than with the Er:YAG laser. High laser pulse energies caused surface fusion which reduced the roughness of micro-roughened titanium surfaces. Likewise, repeated pulses and higher power densities also caused greater surface modifications. The presence of water reduced the influence of laser irradiation on titanium. It may be concluded that conical fibres can reduce unwanted surface modification, and this may be relevant to clinical protocols for debridement or disinfection of titanium dental implants.
Advanced Hydrogels as Wound Dressings
Authors: Tavakoli Shima , Klar Agnes S.
Published: August 2020

Skin is the largest organ of the human body, protecting it against the external environment. Despite high self-regeneration potential, severe skin defects will not heal spontaneously and need to be covered by skin substitutes. Tremendous progress has been made in the field of skin tissue engineering, in recent years, to develop new skin substitutes. Among them, hydrogels are one of the candidates with most potential to mimic the native skin microenvironment, due to their porous and hydrated molecular structure. They can be applied as a permanent or temporary dressing for different wounds to support the regeneration and healing of the injured epidermis, dermis, or both. Based on the material used for their fabrication, hydrogels can be subdivided into two main groups—natural and synthetic. Moreover, hydrogels can be reinforced by incorporating nanoparticles to obtain “in situ” hybrid hydrogels, showing superior properties and tailored functionality. In addition, different sensors can be embedded in hydrogel wound dressings to provide real-time information about the wound environment. This review focuses on the most recent developments in the field of hydrogel-based skin substitutes for skin replacement. In particular, we discuss the synthesis, fabrication, and biomedical application of novel “smart” hydrogels.
Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells
Authors: Balbaied Thanih , Hogan Anna , Moore Eric
Published: August 2020

Alkaline phosphatase (ALP) is one of the main biomarkers that is clinically detected in bone and liver disorders using optical assays. The electrochemical principle is important because point-of-care testing is increasing dramatically and absorbance techniques hardly compete with the medical revolution that is occurring. The detection of ALP using electrochemical detection is contributing to the integration systems field, and hence enhancing the detection of biological targets for pharmaceutical research and design systems. Moreover, in vitro electrochemical measurements use cost effective materials and simple techniques. Graphite screen-printed electrodes and linear sweep voltammetry were used to optimize the electrochemistry of the enzymatic product p-aminophenol using the enzyme kinetic assay. ALP release from embryonic and cancer cells was determined from adhesion cell culture. Additionally, capillary electrophoresis and colorimetric methods were applied for comparison assays. The resulting assays showed a dynamic range of ALP ranging from 1.5 to 1500 U/L, and limit of detection of 0.043 U/L. This was achieved by using 70 μL of the sample and an incubation time of 10 min at an optimal substrate concentration of 9.6 mM of p-aminophenol phosphate. A significant difference (p < 0.05) was measured between the absorbance assays. This paper demonstrates the advantages of the electrochemical assay for ALP release from cells, which is in line with recent trends in gene expression systems using microelectrode array technologies and devices for monitoring electrophysiological activity.
Utility of Circulating Tumor DNA for Detection and Monitoring of Endometrial Cancer Recurrence and Progression
Authors: Moss Esther L. , Gorsia Diviya N. , Collins Anna , Sandhu Pavandeep , Foreman Nalini , Gore Anupama , Wood Joey , Kent Christopher , Silcock Lee , Guttery David S.
Published: August 2020

Despite the increasing incidence of endometrial cancer (EC) worldwide and the poor overall survival of patients who recur, no reliable biomarker exists for detecting and monitoring EC recurrence and progression during routine follow-up. Circulating tumor DNA (ctDNA) is a sensitive method for monitoring cancer activity and stratifying patients that are likely to respond to therapy. As a pilot study, we investigated the utility of ctDNA for detecting and monitoring EC recurrence and progression in 13 patients, using targeted next-generation sequencing (tNGS) and personalized ctDNA assays. Using tNGS, at least one somatic mutation at a variant allele frequency (VAF) > 20% was detected in 69% (9/13) of patient tumors. The four patients with no detectable tumor mutations at >20% VAF were whole exome sequenced, with all four harboring mutations in genes not analyzed by tNGS. Analysis of matched and longitudinal plasma DNA revealed earlier detection of EC recurrence and progression and dynamic kinetics of ctDNA levels reflecting treatment response. We also detected acquired high microsatellite instability (MSI-H) in ctDNA from one patient whose primary tumor was MSI stable. Our study suggests that ctDNA analysis could become a useful biomarker for early detection and monitoring of EC recurrence. However, further research is needed to confirm these findings and to explore their potential implications for patient management.
Association of the Metabolic Score Using Baseline FDG-PET/CT and dNLR with Immunotherapy Outcomes in Advanced NSCLC Patients Treated with First-Line Pembrolizumab
Authors: Seban Romain-David , Assié Jean-Baptiste , Giroux-Leprieur Etienne , Massiani Marie-Ange , Soussan Michael , Bonardel Gérald , Chouaid Christos , Playe Margot , Goldfarb Lucas , Duchemann Boris , Mezquita Laura , Girard Nicolas , Champion Laurence
Published: August 2020

Background: We aimed to assess the clinical utility of a previously published score combining the total metabolic tumor volume (TMTV) on baseline FDG-PET/CT and pretreatment derived from the neutrophils to lymphocytes ratio (dNLR) for prognostication in NSCLC patients undergoing first-line immunotherapy (IT). Methods: In this multicenter retrospective study, 63 advanced NSCLC patients with a PD-L1 tumor proportion score (TPS) ≥50%, who underwent FDG-PET/CT before first-line IT, treated from January 2017 to September 2019, were enrolled. Associations between this score and the progression-free survival (PFS), overall survival (OS), disease control rate (DCR), and overall response rate (ORR) were evaluated. Results: The median (m) PFS and mOS were 7.7 (95% CI 4.9–10.6) and 12.1 (8.6–15.6) months, respectively, and DCR and ORR were 65% and 58%, respectively. mOS was 17.9 months (14.6 not reached) for the good group versus 13.8 (95%CI 8.4–18.9) and 6.6 (CI 2.0–11.2) months for the intermediate and poor groups, respectively. mPFS was 15.1 (95%CI 12.1–20.0) months for the good group versus 5.2 (1.9–8.5) and 1.9 (95%CI 1.3–2.5) months for the intermediate and poor groups, respectively. The poor prognosis group was associated with DCR and ORR (p < 0.05). Conclusions: The metabolic score combining TMTV on the baseline FDG-PET/CT scan and pretreatment dNLR was associated with the survival and response in a cohort of advanced NSCLC patients with ≥50% PD-L1 receiving frontline IT.
Selectins: An Important Family of Glycan-Binding Cell Adhesion Molecules in Ovarian Cancer
Authors: Hassan Ayon A. , Artemenko Margarita , Tang Maggie K.S. , Wong Alice S.T.
Published: August 2020

Ovarian cancer is the most lethal gynecological malignancy worldwide. Unlike most other tumor types that metastasize via the vasculature, ovarian cancer metastasizes predominantly via the transcoelomic route within the peritoneal cavity. As cancer metastasis accounts for the majority of deaths, there is an urge to better understand its determinants. In the peritoneal cavity, tumor-mesothelial adhesion is an important step for cancer dissemination. Selectins are glycan-binding molecules that facilitate early steps of this adhesion cascade by mediating heterotypic cell-cell interaction under hydrodynamic flow. Here, we review the function and regulation of selectins in peritoneal carcinomatosis of ovarian cancer, and highlight how dysregulation of selectin ligand biogenesis affects disease outcome. Further, we will introduce the latest tools in studying selectin-glycan interaction. Finally, an overview of potential therapeutic intervention points that may lead to the development of efficacious therapies for ovarian cancer is provided.
Laryngeal Compartmentalization Does Not Affect the Prognosis of T3-T4 Laryngeal Cancer Treated by Upfront Total Laryngectomy
Authors: Marchi Filippo , Missale Francesco , Sampieri Claudio , Filauro Marta , Iandelli Andrea , Parrinello Giampiero , Incandela Fabiola , Smeele Ludwig E. , van den Brekel Michiel W. M. , Del Bon Francesca , Nicolai Piero , Piazza Cesare , Peretti Giorgio
Published: August 2020

A picture is emerging in which advanced laryngeal cancers (LCs) are potentially not homogeneous and may be characterized by subpopulations which, if identified, could allow selection of patients amenable to organ preservation treatments in contrast to those to be treated with total laryngectomy (TL). This work aims to analyze a multicentric cohort of T3-T4a LCs treated by upfront TL, investigating the clinical and pathological features that can best predict oncologic outcomes. A total of 149 previously untreated patients who underwent TL for T3-T4a LC at four institutions were analyzed. Survival and disease-control were considered as the main outcomes. A secondary end-point was the identification of covariates associated with nodal status, investigating also the tumor thickness. T and N categories were significantly associated with both overall and disease-specific survival. The number of positive nodes and tracheal involvement were associated with loco-regional failure; post-cricoid area invasion and extra-nodal extension with distant failure. Posterior laryngeal compartment involvement was not a significant prognostic feature, by either univariable and multivariable analyses. These results support the conclusion that laryngeal compartmentalization has no impact on survival in patients treated by upfront TL and the current TNM staging system remains a robust prognosticator in advanced LC.
Novel Somatic Genetic Variants as Predictors of Resistance to EGFR-Targeted Therapies in Metastatic Colorectal Cancer Patients
Authors: Riera Pau , Rodríguez-Santiago Benjamín , Lasa Adriana , Gonzalez-Quereda Lidia , Martín Berta , Salazar Juliana , Sebio Ana , Virgili Anna C. , Minguillón Jordi , Camps Cristina , Surrallés Jordi , Páez David
Published: August 2020

Background: About 40% of RAS/BRAF wild-type metastatic colorectal cancer (mCRC) patients undergoing anti-EGFR-based therapy have poor outcomes. Treatment failure is not only associated with poorer prognosis but higher healthcare costs. Our aim was to identify novel somatic genetic variants in the primary tumor and assess their effect on anti-EGFR response. Patients and Methods: Tumor (somatic) and blood (germline) DNA samples were obtained from two well-defined cohorts of mCRC patients, those sensitive and those resistant to EGFR blockade. Genetic variant screening of 43 EGFR-related genes was performed using targeted next-generation sequencing (NGS). Relevant clinical data were collected through chart review to assess genetic results. Results: Among 61 patients, 38 were sensitive and 23 were resistant to treatment. We identified eight somatic variants that predicted non-response. Three were located in insulin-related genes (I668N and E1218K in IGF1R, T1156M in IRS2) and three in genes belonging to the LRIG family (T152T in LRIG1, S697L in LRIG2 and V812M in LRIG3). The remaining two variants were found in NRAS (G115Efs*46) and PDGFRA (T301T). We did not identify any somatic variants related to good response. Conclusions: This study provides evidence that novel somatic genetic variants along the EGFR-triggered pathway could modulate the response to anti-EGFR drugs in mCRC patients. It also highlights the influence of insulin-related genes and LRIG genes on anti-EGFR efficacy. Our findings could help characterize patients who are resistant to anti-EGFR blockade despite harboring RAS/BRAF wild-type tumors.
A Multi-Analyte Approach for Improved Sensitivity of Liquid Biopsies in Prostate Cancer
Authors: Hofmann Lilli , Sallinger Katja , Haudum Christoph , Smolle Maria , Heitzer Ellen , Moser Tina , Novy Michael , Gesson Kevin , Kroneis Thomas , Bauernhofer Thomas , El-Heliebi Amin
Published: August 2020

Novel androgen receptor (AR) signaling inhibitors have improved the treatment of castration-resistant prostate cancer (CRPC). Nonetheless, the effect of these drugs is often time-limited and eventually most patients become resistant due to various AR alterations. Although liquid biopsy approaches are powerful tools for early detection of such therapy resistances, most assays investigate only a single resistance mechanism. In combination with the typically low abundance of circulating biomarkers, liquid biopsy assays are therefore informative only in a subset of patients. In this pilot study, we aimed to increase overall sensitivity for tumor-related information by combining three liquid biopsy approaches into a multi-analyte approach. In a cohort of 19 CRPC patients, we (1) enumerated and characterized circulating tumor cells (CTCs) by mRNA-based in situ padlock probe analysis, (2) used RT-qPCR to detect cancer-associated transcripts (e.g., AR and AR-splice variant 7) in lysed whole blood, and (3) conducted shallow whole-genome plasma sequencing to detect AR amplification. Although 44–53% of patient samples were informative for each assay, a combination of all three approaches led to improved diagnostic sensitivity, providing tumor-related information in 89% of patients. Additionally, distinct resistance mechanisms co-occurred in two patients, further reinforcing the implementation of multi-analyte liquid biopsy approaches.
Photocatalytic Activity of Aeroxide TiO2 Sensitized by Natural Dye Extracted from Mangosteen Peel
Authors: Ghosh Malini , Chowdhury Pankaj , Ray Ajay K.
Published: August 2020

Natural dye sensitizers are environment-friendly and inexpensive substances that could be used for photocatalytic decontamination of organic pollutants. In this study, a natural dye extracted from mangosteen peel, containing a significant amount of anthocyanin dye, has been successfully employed to sensitize aeroxide TiO2 to lower its bandgap, thereby making the process visible sunlight-driven. We have demonstrated the photocatalytic activity of mangosteen dye-sensitized-TiO2 (MS-TiO2) under visible solar light by studying the degradation of methylene blue (MB), a well-studied model compound. A multivariate parametric study was performed using factorial design methodology with three factors—pH, MS-TiO2 dosage, and visible light intensity. The study indicated that pH and MS-TiO2 dosage are the two most dominant factors for MB degradation under visible solar light. The kinetic rate constant and adsorption equilibrium constant were determined, and a Langmuir-Hinshelwood-type equation was proposed to describe MB degradation on MS-TiO2 under visible solar light. Apparent quantum yield was also reported for the MS-TiO2 photocatalyst at optimum experimental conditions.
Piperidinium and Pyrrolidinium Ionic Liquids as Precursors in the Synthesis of New Platinum Catalysts for Hydrosilylation
Authors: Jankowska-Wajda Magdalena , Bartlewicz Olga , Pietras Przemysław , Maciejewski Hieronim
Published: August 2020

Six new air-stable anionic platinum complexes were synthesized in simple reactions of piperidinium [BMPip]Cl or pyrrolidinium [BMPyrr]Cl ionic liquids with platinum compounds ([Pt(cod)Cl2] or K2[PtCl6]). All these compounds were subjected to isolation and spectrometric characterization using NMR and ESI-MS techniques. Furthermore, the determination of melting points and thermal stability of the above derivatives was performed with the use of thermogravimetric analysis. The catalytic performance of the synthesized complexes was tested in hydrosilylation of 1-octene and allyl glycidyl ether with 1,1,1,3,5,5,5-heptamethyltrisiloxane. The study has shown that they have high catalytic activity and are insoluble in the reaction medium which enabled them to isolate and reuse them in consecutive catalytic cycles. The most active complex [BMPip]2[PtCl6] makes it possible to conduct at least 10 catalytic runs without losing activity which makes it an attractive alternative not only to commonly used homogeneous catalysts, but also to heterogeneous catalysts for hydrosilylation processes. The activity of the studied catalysts is also affected by the kind of anion and, to some extent, the kind of cation.