Synthesis, QSAR study and Pharmacological Evaluation of Novel Triazolidine-2-thione Analogues as Antimicrobial, Anti-inflammatory and Antioxidant agents
Authors: Sharma Abhishek , Bhutani Rubina , Gupta Akanksha , Dutta Manni 
Published: March 2023

Background:

The triazole analogues are molecules of immense attraction because of their wide pharmacological applications.

background:

The triazole analogues are molecules of immense attraction because of wide pharmacological applications

Methods:

Present research deals with the synthesis of triazole-2-thione analogues and their QSAR study. The synthesized analogs are also evaluated for their antimicrobial, anti-inflammatory, and antioxidant effect.

Results:

It was revealed that the benzamide analogues (3a, 3d) and triazolidine analogue (4b) were found to be most active against P. aeruginosa and E. coli with pMIC values of 1.69, 1.69 and 1.72, respectively. The antioxidant study of the derivatives showed that 4b was the most active antioxidant with 79% protein denaturation inhibition. The highest anti-inflammatory activity was shown by 3f, 4a and 4f.

method:

Serial dilution method was followed for the antimicrobial evaluation. The antimicrobial assay of the synthesized compounds was conducted on three bacterial strains, i.e. Gram positive Bacillus subtilis and gram negative i.e. Eschercheria coli, Pseudomonas aeruginosa and two fungal strains, i.e. Asperigillus niger and Candida albicans respectively. In-Vitro Anti-Inflammatory Activity was evaluated by Inhibition of albumin denaturation. The free radical scavenging activity of the synthesized compounds was determined by using in vitro assay of 2, 2-diphenyl-1 –picrylhydrazyl (DPPH).We have also applied multiple linear regressions (MLR) which is a classical technique in solving QSAR problems to develop the QSAR models and variable selection was achieved with genetic algorithm using BUILDQSAR software. [18]

Conclusion:

This study provides certain potent leads for further development of more potential anti-inflammatory, antioxidant and antimicrobial agents.

result:

It was revealed that the benzamide analogues (3a, 3d) and triazolidine analogue (4b) were found to be most active against P. aeruginosa and E.coli with pMIC values of 1.69, 1.69 and 1.72 respectively. The antioxidant study of the derivatives showed that 4b was most active antioxidant with 79% protein denaturation inhibition. Highest anti-inflammatory activity was shown by 3f, 4a and 4f.

conclusion:

In the present work, a novel series of substituted triazolidine-2-thione analogues were synthesized in appreciable yields. The compounds were evaluated for their antimicrobial, anti-inflammatory and antioxidant activity. The antimicrobial activity was carried out against three bacterial strains and two fungal strains. It was found that 3c and 4d were potent antimicrobial agents against different bacterial and fungal strains. Also, the antioxidant study of the derivatives showed that 4b was most active antioxidant with 79% protein denaturation inhibition.

other:

This work includes the synthesis, QSAR study of triazolidine-2-thione scaffold and exploring its antimicrobial, anti-oxidant and anti-inflammatory potential, thus constituting it into a lead. Further, this paper will aid in the development of new drugs based on triazolidine-2-thione core.