bluuebunny/crossref_metadata_2025 huggingface dataset:
All-Optically Controllable Photonic Crystals Based on Chiral-Azobenzene-Doped Blue Phase Liquid Crystals
Authors: Jiang Shun-An , Wu Chan-Hong , Mo Ting-Shan , Huang Shuan-Yu , Lin Jia-De , Lee Chia-Rong
Published: October 2020

In this study, the all-optical control properties of photonic crystals based on self-assembled chiral-azobenzene-doped blue phase liquid crystals (CA-BPLCs) were investigated. The difference in the photothermal characteristics of CA-BPLCs with and without homogeneous surface alignment was discussed. Results revealed that surface alignment could induce more uniform and diverse blue phase (BP) structures, including BPII, BPI, and BPS-like phases during cooling. Consequently, the temperature range of BP was wider than that of the sample without surface alignment. All-optical control experiments with light illumination were then performed on the aligned or nonaligned CA-BPLC samples. During continuous irradiation with light beams at wavelengths of 405 and 450 nm, CA dopants underwent trans→cis and cis→trans back photoisomerizations, respectively. These processes promoted isothermal phase transition and wavelength shifting, which further enabled the all-optical control of the CA-BPLC samples. Various optical control modes of BPLC could be achieved through phase change and wavelength shifting by appropriately selecting the working temperature and surface treatment of BPLC. This study could be further used as a basis for developing photoswitchable and tunable BPLC photonic devices, such as light-controllable gratings, filters, mirrors, and lasers.
Efficacy and Safety of Non-Anesthesiologist Administration of Propofol Sedation in Endoscopic Ultrasound: A Propensity Score Analysis
Authors: Facciorusso Antonio , Turco Antonio , Barnabà Carlo , Longo Grazia , Dipasquale Graziano , Muscatiello Nicola
Published: October 2020

In spite of promising preliminary results, evidence supporting the use of non-anesthesiologist-administered propofol sedation (NAAP) in endoscopic ultrasound (EUS) procedures is still limited. The aim of this manuscript was to examine the safety and efficacy of NAAP as compared to anesthesiologist-administered propofol sedation in EUS procedures performed in a referral center. Out of 832 patients referred to our center between 2016 and 2019, after propensity score matching two groups were compared: 305 treated with NAAP and 305 controls who underwent anesthesiologist-administered propofol sedation. The primary outcome was the rate of major complications. The median age was 67 years and the proportion of patients with comorbidities was 31.8% in both groups. One patient in each group (0.3%) experienced a major complication, whereas minor complications were observed in 13 patients in the NAAP group (4.2%) and 10 patients in the control group (3.2%; p = 0.52). Overall pain during the procedure was 2.3 ± 1 in group 1 and 1.8 ± 1 in group 2 (p = 0.67), whereas pain/discomfort upon awakening was rated as 1 ± 0.5 in both groups (p = 0.72). NAAP is safe and effective even in advanced EUS procedures. Further randomized-controlled trials (RCTs) are warranted to confirm these findings.
Deep Learning-Based Security Verification for a Random Number Generator Using White Chaos
Authors: Li Cai , Zhang Jianguo , Sang Luxiao , Gong Lishuang , Wang Longsheng , Wang Anbang , Wang Yuncai
Published: October 2020

In this paper, a deep learning (DL)-based predictive analysis is proposed to analyze the security of a non-deterministic random number generator (NRNG) using white chaos. In particular, the temporal pattern attention (TPA)-based DL model is employed to learn and analyze the data from both stages of the NRNG: the output data of a chaotic external-cavity semiconductor laser (ECL) and the final output data of the NRNG. For the ECL stage, the results show that the model successfully detects inherent correlations caused by the time-delay signature. After optical heterodyning of two chaotic ECLs and minimal post-processing are introduced, the model detects no patterns among corresponding data. It demonstrates that the NRNG has the strong resistance against the predictive model. Prior to these works, the powerful predictive capability of the model is investigated and demonstrated by applying it to a random number generator (RNG) using linear congruential algorithm. Our research shows that the DL-based predictive model is expected to provide an efficient supplement for evaluating the security and quality of RNGs.
Financial Inclusion and Firms Growth in Manufacturing Sector: A Threshold Regression Analysis in Selected Asean Countries
Authors: Nizam Rosmah , Abdul Karim Zulkefly , Sarmidi Tamat , Abdul Rahman Aisyah
Published: October 2020

This paper examines the effect of financial inclusion on the firm growth of the manufacturing sector (513 firms) in selected ASEAN countries (Malaysia, Philippines, and Vietnam) using a cross-section threshold estimation technique. The levels of financial inclusion across firms were measured based on the distribution of financial services (access to credit). The main findings revealed that there is a non-monotonic effect of financial inclusion on the firm’s growth. These findings show that the impact of financial inclusion on firm growth in the manufacturing sector is significantly positive below a threshold point, and turns to significantly negative after a certain threshold point has been reached. These new findings suggest that manufacturing firm owners and banking institutions should deepen their financial inclusion efforts, and limit the distribution of credit access within the optimum value or threshold level in promoting the growth of the firm.
The Impact of Imitation Strategies, Managerial and Entrepreneurial Skills on Startups’ Entrepreneurial Innovation
Authors: Tsolakidis Panagiotis , Mylonas Naoum , Petridou Eugenia
Published: October 2020

The scope of this paper is to investigate whether imitation strategies adopted by startups are effective in the pursuit of enhanced entrepreneurial innovation. To this end, a literature review was carried out in order to develop a research framework with factors related to imitation predicting entrepreneurial innovation. Moreover, managerial skills and entrepreneurial skills were incorporated as predictors of entrepreneurial innovation. In this respect, a structured questionnaire was developed to address these research objectives, based on scales tested in previous studies. In 2020, a survey was conducted on 486 startup owners operating in Greece, and a total of 289 responses were received. A hierarchical regression analysis was employed in order to examine the research framework. In congruence with the hypotheses, the findings demonstrated that outcome-based imitation strategies and trait-based imitation strategies positively affect the development of entrepreneurial innovation. Moreover, the positive impact of managerial and entrepreneurial skills on the development of entrepreneurial innovation was also confirmed. In contrast, the findings suggest that frequency-based imitation strategies negatively predict entrepreneurial innovation. However, outcome-based imitation and trait-based imitation strategies have been shown as the determinants with a positive impact on entrepreneurial innovation.
Selection of Solar Concentrator Design Concepts for Planar Photoelectrochemical Water Splitting Devices
Authors: Caron Simon , Röger Marc , Wullenkord Michael
Published: October 2020

Photoelectrochemical water splitting is a promising pathway for solar-driven hydrogen production with a low environmental footprint. The utilization of solar concentrators to supply such water splitting devices with concentrated solar irradiation offers great potential to enhance the economic viability of water splitting at “sunny” site locations. In this work, we defined a set of functional requirements for solar concentrators to assess their suitability to power such water splitting devices, taking into account concentrator optical performance, device coupling efficiency, perceived system complexity, as well as technological costs and risks. We identified, classified and compared a broad range of existing solar concentrator design concepts. Our geometrical analysis, performed on a yearly basis with a one-minute time step, shows that two-axis tracking concentrators with water splitting devices positioned parallel to the optical aperture plane exhibit the highest potential, given the initial conditions applied for the device tilt constraints. Demanding an angle of at least 20° between horizontal and the front side of the water splitting device, allows the device to be operational for 97% of the daylight time in Seville, Spain. The relative loss with respect to the available direct normal irradiance is estimated to 6%. Results moderately depend on the location of application, but generally confirm that the consideration of tilt angle constraints is essential for a comprehensive performance assessment of photoelectrochemical water splitting driven by concentrated sunlight.
Experimental Evaluation of the Effect of the Anode Diffusion Layer Properties on the Performance of a Passive Direct Methanol Fuel Cell
Authors: Braz Beatriz A. , Oliveira Vânia B. , Pinto Alexandra M. F. R.
Published: October 2020

Passive direct methanol fuel cells (pDMFCs) are promising devices to replace the conventional batteries in portable electronic devices, due to their higher energy densities, autonomies, and instant recharging. However, some challenges, such as their costs, efficiency, and durability, need to be overcome before their commercialization. Towards that, this work presents the effect of the anode diffusion layer (ADL) properties on the performance of a pDMFC using a membrane electrode assembly (MEA) with reduced loadings on both anode and cathode catalysts (3 mg/cm2 Pt/Ru on the anode and 1.3 mg/cm2 of Pt on the cathode). The pDMFC behavior was evaluated through polarization and electrochemical impedance spectroscopy measurements, which allow identifying and quantifying the different losses that affect these systems. The results showed better performances when a diffusion layer with a dual-layer structure was used using higher methanol concentrations. The maximum power density achieved was 3.00 mW/cm2, using carbon cloth with a microporous layer, CC_MPL, as ADL, and a methanol concentration of 5 M. In this work, a tailored and low-cost MEA, using the materials available in the market, was proposed to achieve higher performances working under higher methanol concentrations. This work demonstrates that performing modifications on the fuel cell structure/design is an efficient way to achieve optimized performances.
A Power Hardware-in-the-Loop Based Method for FAPR Compliance Testing of the Wind Turbine Converters Control
Authors: Ahmad Zameer , Torres Jose Rueda , Veera Kumar Nidarshan , Rakhshani Elyas , Palensky Peter , van der Meijden Mart
Published: October 2020

A task for new power generation technologies, interfaced to the electrical grid by power electronic converters, is to stiffen the rate of change of frequency (RoCoF) at the initial few milliseconds (ms) after any variation of active power balance. This task is defined in this article as fast active power regulation (FAPR), a generic definition of the FAPR is also proposed in this study. Converters equipped with FAPR controls should be tested in laboratory conditions before employment in the actual power system. This paper presents a power hardware-in-the-loop (PHIL) based method for FAPR compliance testing of the wind turbine converter controls. The presented PHIL setup is a generic test setup for the testing of all kinds of control strategies of the grid-connected power electronic converters. Firstly, a generic PHIL testing methodology is presented. Later on, a combined droop- anFd derivative-based FAPR control has been implemented and tested on the proposed PHIL setup for FAPR compliance criteria of the wind turbine converters. The compliance criteria for the FAPR of the wind turbine converter controls have been framed based on the literature survey. Improvement in the RoCoF and and maximum underfrequency deviation (NADIR) has been observed if the wind turbine converter controls abide by the FAPR compliance criteria.
Pressure-Transient Performances of Fractured Horizontal Wells in the Compartmentalized Heterogeneous Unconventional Reservoirs
Authors: Fan Dongyan , Sun Hai , Yao Jun , Zeng Hui , Yan Xia , Sun Zhixue
Published: October 2020

In order to investigate pressure performance of multiple fractured horizontal wells (MFHWs) penetrating heterogeneous unconventional reservoir and avoid the high computational cost of numerical simulation, a semi-analytical model for MFHWs combining Green function solution and boundary element method has been obtained, where the reservoir is divided into different homogeneous substructures and coupled at interface boundaries by plane source function in a closed rectangular parallelepiped. Hydraulic fractures are assumed uniform flux and dual porosity model is used for natural fractures system. Then the model is validated by compared with analytical solution of MFHWs in a homogeneous reservoir and trilinear flow model, which shows that this model can achieve high accuracy even with a small interface discretization number, and it can consider the radial flow around each hydraulic fractures. Finally, the pressure responses with heterogeneous parameters of reservoirs are discussed including heterogeneous permeability, non-uniform block-length and fracture half-length distribution as well as dual porosity parameters like elastic storage ratio and crossflow ratio.
A Novel Method to Achieve Selective Emitter Using Surface Morphology for PERC Silicon Solar Cells
Authors: Ju Minkyu , Park Jeongeun , Cho Young Hyun , Kim Youngkuk , Lim Donggun , Cho Eun-Chel , Yi Junsin
Published: October 2020

Recently, selective emitter (SE) technology has attracted renewed attention in the Si solar cell industry to achieve an improved conversion efficiency of passivated-emitter rear-contact (PERC) cells. In this study, we presented a novel technique for the SE formation by controlling the surface morphology of Si wafers. SEs were formed simultaneously, that is, in a single step for the doping process on different surface morphologies, nano/micro-surfaces, which were formed during the texturing processes; in the same doping process, the nano- and micro-structured areas showed different sheet resistances. In addition, the difference in sheet resistance between the heavily doped and shallow emitters could be controlled from almost 0 to 60 Ω/sq by changing the doping process conditions, pre-deposition and driving time, and temperature. Regarding cell fabrication, wafers simultaneously doped in the same tube were used. The sheet resistance of the homogeneously doped-on standard micro-pyramid surface was approximately 82 Ω/sq, and those of the selectively formed nano/micro-surfaces doped on were on 62 and 82 Ω/sq, respectively. As a result, regarding doped-on selectively formed nano/micro-surfaces, SE cells showed a JSC increase (0.44 mA/cm2) and a fill factor (FF) increase (0.6%) with respect to the homogeneously doped cells on the micro-pyramid surface, resulting in about 0.27% enhanced conversion efficiency.
Herb-Layer Dynamics in an Old-Growth Forest: Vegetation–Environment Relationships and Response to Invasion-Related Perturbations
Authors: Buskey Taylor M. , Maloney Margaret E. , Chapman Julia I. , McEwan Ryan W.
Published: October 2020

Temperate forests of eastern North America are subject to multiple invasions from non-native species that have the potential to drive long-term dynamics in biodiversity. Garlic mustard (Alliaria petiolata (M. Bieb.) Cavara and Grande) is an invasive plant in many deciduous forests, and management efforts often focus on removing this species to initiate native species restoration. Agrilus planipennis Fairmaire (emerald ash borer; Coleoptera: Buprestidae) is a non-native insect pest that has caused substantial loss of ash trees (Fraxinus spp. L.) in North America. Our goal was to understand how the herbaceous layer in an old-growth forest responded to the removal of a significant invasion of A. petiolata and the loss of Fraxinus spp. due to A. planipennis. Herbaceous diversity and environmental parameters were measured in 32 permanent plots (1 m2 each) from 2012 to 2020 in an old-growth forest remnant that had experienced A. petiolata invasion and subsequent removal as well as mortality of Fraxinus spp. due to A. planipennis. Near-total loss of Fraxinus spp. as a canopy tree was not associated with changes in the understory light environment, possibly due to rapid canopy closure by adjacent trees not susceptible to the insect. Alliaria petiolata removal was associated with changes in herbaceous species richness and possibly shifts in individual species importance. Vegetation–environment relationships remained stable throughout the sampling period, suggesting that resource-related factors that structure the herb layer prevailed throughout the changes associated with Fraxinus spp. mortality and A. petiolata management. From a natural area management perspective, our data offer support for the idea that A. petiolata removal influences herb-layer diversity and indicate that in stands with a diverse tree community, the loss of Fraxinus spp. may not directly influence understory biodiversity.
Reforestation and Deforestation in Northern Luzon, Philippines: Critical Issues as Observed from Space
Authors: Perez Gay Jane , Comiso Josefino C. , Aragones Lemnuel V. , Merida Harry C. , Ong Perry S.
Published: October 2020

Among the richest in biodiversity globally has been the Philippine rainforest, which used to cover about 90% of the country’s land area. During the last few decades, the forest cover has been reduced to less than 10% of the original, only a fraction of which is old-growth forest. The negative impacts of deforestation led to the launching of the National Greening Program (NGP) that involved the planting of more than a billion seedlings over a few million hectares of land from 2011 to 2016. To assess the success of the NGP, satellite data from Landsat and the Moderate Resolution Imaging Spectroradiometer (MODIS) were analyzed before, during, and after the NGP. Reforestation in the NGP sites was examined concurrently with observed deforestation in Luzon using forest loss data derived from Landsat for the period 2001 to 2018. The results show that losses declined from 2011 to 2015 but increased from 2016 to 2018. Because of such losses, the net effect is a balance of reforestation and deforestation or no significant gain from the NGP. Case studies were done in three sites in the Sierra Madre forest, where half of the remaining old-growth forest is located, using a combination of Landsat and Very High Resolution (VHR) data. The Landsat data were classified into closed forest, open forest, and other vegetation cover types. The conversion from one vegetation cover type to another was evaluated through the use of the Sankey Diagram. While some non-forest types became open or closed forests, the loss of open or closed forests is more pronounced. VHR data reveal critical issues happening within the NGP sites during the NGP period. More comprehensive data from MODIS also confirm that there was no significant increase in the forest cover in Luzon, Sierra Madre, and Cordillera from 2001 to 2018.
Large-Scale Screening of Thiol and Fermentative Aroma Production during Wine Alcoholic Fermentation: Exploring the Effects of Assimilable Nitrogen and Peptides
Authors: Duc Camille , Maçna Faïza , Sanchez Isabelle , Galeote Virginie , Delpech Stéphane , Silvano Anthony , Mouret Jean-Roch
Published: October 2020

In alcoholic fermentation, under oenological conditions, the environmental parameters impacting fermentation kinetics and aroma production have been widely studied. The nitrogen content of grape must was found to be one of the most important parameters for both of these aspects of fermentation. Many studies have been performed on the effect of mineral nitrogen addition. However, it has increasingly been observed that the nature of the nitrogen added leads to different results. Our work focused on the effects of peptide addition on both fermentation kinetics and aroma production. Peptides are one of the less well understood sources of assimilable nitrogen, as their incorporation by yeast remains unclear. In this study, we compared the effect of the addition of a “classic” assimilable nitrogen source (ammonium + amino acids) with that of peptide addition in both white and red must fermentation by screening 18 Saccharomyces cerevisiae strains in total. Our data show that peptide addition enhances fermentation kinetics and leads to specific changes in the production of fermentative aromas. The impact of peptides on thiol synthesis is rather limited.
An Assessment of Annual Mortality Attributable to Ambient PM2.5 in Bangkok, Thailand
Authors: Fold Nathaniel R. , Allison Mary R. , Wood Berkley C. , Thao Pham T. B. , Bonnet Sebastien , Garivait Savitri , Kamens Richard , Pengjan Sitthipong
Published: October 2020

Multiple studies indicate that PM2.5 is the most deleterious air pollutant for which there are ambient air quality standards. Daily concentrations of PM2.5 in Bangkok, Thailand, continuously exceed the World Health Organization (WHO) and the Thai National Ambient Air Quality Standards (NAAQSs). Bangkok has only recently begun to measure concentrations of PM2.5. To overcome this paucity of data, daily PM2.5/PM10 ratios were generated over the period 2012–2018 to interpolate missing values. Concentration-response coefficients (β values) for PM2.5 versus non-accidental, cardiopulmonary, and lung cancer mortalities were derived from the literature. Values were also estimated and were found to be comparable to those reported in the literature for a Chinese population, but considerably lower than those reported in the literature from the United States. These findings strongly suggest that specific regional β values should be used to accurately quantify the number of premature deaths attributable to PM2.5 in Asian populations. Health burden analysis using the Environmental Benefits Mapping and Analysis Program (BenMAP) showed that PM2.5 concentration in Bangkok contributes to 4240 non-accidental, 1317 cardiopulmonary, and 370 lung cancer mortalities annually. Further analysis showed that the attainment of PM2.5 levels to the NAAQSs and WHO guideline would reduce annual premature mortality in Bangkok by 33%and 75%, respectively.
A Machine Learning-Based Approach for Spatial Estimation Using the Spatial Features of Coordinate Information
Authors: Ahn Seongin , Ryu Dong-Woo , Lee Sangho
Published: October 2020

With the development of machine learning technology, research cases for spatial estimation through machine learning approach (MLA) in addition to the traditional geostatistical techniques are increasing. MLA has the advantage that spatial estimation is possible without stationary hypotheses of data, but it is possible for the prediction results to ignore spatial autocorrelation. In recent studies, it was considered by using a distance matrix instead of raw coordinates. Although, the performance of spatial estimation could be improved through this approach, the computational complexity of MLA increased rapidly as the number of sample points increased. In this study, we developed a method to reduce the computational complexity of MLA while considering spatial autocorrelation. Principal component analysis is applied to it for extracting spatial features and reducing dimension of inputs. To verify the proposed approach, indicator Kriging was used as a benchmark model, and each performance of MLA was compared when using raw coordinates, distance vector, and spatial features extracted from distance vector as inputs. The proposed approach improved the performance compared to previous MLA and showed similar performance compared with Kriging. We confirmed that extracted features have characteristics of rigid classification in spatial estimation; on this basis, we conclude that the model could improve performance.
Targeting DNA Repair Pathways in Hematological Malignancies
Authors: Alhmoud Jehad F. , Mustafa Ayman G. , Malki Mohammed Imad
Published: October 2020

DNA repair plays an essential role in protecting cells that are repeatedly exposed to endogenous or exogenous insults that can induce varying degrees of DNA damage. Any defect in DNA repair mechanisms results in multiple genomic changes that ultimately may result in mutation, tumor growth, and/or cell apoptosis. Furthermore, impaired repair mechanisms can also lead to genomic instability, which can initiate tumorigenesis and development of hematological malignancy. This review discusses recent findings and highlights the importance of DNA repair components and the impact of their aberrations on hematological malignancies.
A Myosin II-Based Nanomachine Devised for the Study of Ca2+-Dependent Mechanisms of Muscle Regulation
Authors: Pertici Irene , Bianchi Giulio , Bongini Lorenzo , Lombardi Vincenzo , Bianco Pasquale
Published: October 2020

The emergent properties of the array arrangement of the molecular motor myosin II in the sarcomere of the striated muscle, the generation of steady force and shortening, can be studied in vitro with a synthetic nanomachine made of an ensemble of eight heavy-meromyosin (HMM) fragments of myosin from rabbit psoas muscle, carried on a piezoelectric nanopositioner and brought to interact with a properly oriented actin filament attached via gelsolin (a Ca2+-regulated actin binding protein) to a bead trapped by dual laser optical tweezers. However, the application of the original version of the nanomachine to investigate the Ca2+-dependent regulation mechanisms of the other sarcomeric (regulatory or cytoskeleton) proteins, adding them one at a time, was prevented by the impossibility to preserve [Ca2+] as a free parameter. Here, the nanomachine is implemented by assembling the bead-attached actin filament with the Ca2+-insensitive gelsolin fragment TL40. The performance of the nanomachine is determined both in the absence and in the presence of Ca2+ (0.1 mM, the concentration required for actin attachment to the bead with gelsolin). The nanomachine exhibits a maximum power output of 5.4 aW, independently of [Ca2+], opening the possibility for future studies of the Ca2+-dependent function/dysfunction of regulatory and cytoskeletal proteins.
Altered Gap Junction Network Topography in Mouse Models for Human Hereditary Deafness
Authors: Eitelmann Sara , Petersilie Laura , Rose Christine R. , Stephan Jonathan
Published: October 2020

Anisotropic gap junctional coupling is a distinct feature of astrocytes in many brain regions. In the lateral superior olive (LSO), astrocytic networks are anisotropic and oriented orthogonally to the tonotopic axis. In CaV1.3 knock-out (KO) and otoferlin KO mice, where auditory brainstem nuclei are deprived from spontaneous cochlea-driven neuronal activity, neuronal circuitry is disturbed. So far it was unknown if this disturbance is also accompanied by an impaired topography of LSO astrocyte networks. To answer this question, we immunohistochemically analyzed the expression of astrocytic connexin (Cx) 43 and Cx30 in auditory brainstem nuclei. Furthermore, we loaded LSO astrocytes with the gap junction-permeable tracer neurobiotin and assessed the network shape and orientation. We found a strong elevation of Cx30 immunoreactivity in the LSO of CaV1.3 KO mice, while Cx43 levels were only slightly increased. In otoferlin KO mice, LSO showed a slight increase in Cx43 as well, whereas Cx30 levels were unchanged. The total number of tracer-coupled cells was unaltered and most networks were anisotropic in both KO strains. In contrast to the WTs, however, LSO networks were predominantly oriented parallel to the tonotopic axis and not orthogonal to it. Taken together, our data demonstrate that spontaneous cochlea-driven neuronal activity is not required per se for the formation of anisotropic LSO astrocyte networks. However, neuronal activity is required to establish the proper orientation of networks. Proper formation of LSO astrocyte networks thus necessitates neuronal input from the periphery, indicating a critical role of neuron-glia interaction during early postnatal development in the auditory brainstem.
Clinical Trials of Stem Cell Therapy for Cerebral Ischemic Stroke
Authors: Kawabori Masahito , Shichinohe Hideo , Kuroda Satoshi , Houkin Kiyohiro
Published: October 2020

Despite recent developments in innovative treatment strategies, stroke remains one of the leading causes of death and disability worldwide. Stem cell therapy is currently attracting much attention due to its potential for exerting significant therapeutic effects on stroke patients. Various types of cells, including bone marrow mononuclear cells, bone marrow/adipose-derived stem/stromal cells, umbilical cord blood cells, neural stem cells, and olfactory ensheathing cells have enhanced neurological outcomes in animal stroke models. These stem cells have also been tested via clinical trials involving stroke patients. In this article, the authors review potential molecular mechanisms underlying neural recovery associated with stem cell treatment, as well as recent advances in stem cell therapy, with particular reference to clinical trials and future prospects for such therapy in treating stroke.
Towards a New Paradigm of Federated Electronic Health Records in Palestine
Authors: El Jabari Carol , Macedo Mario , Al-jabari Mohanad O.
Published: October 2020

While efforts are underway to create a sound system of electronic health records in Palestinian health institutions, there remain obstacles and challenges. Given modern day demands on health systems, we propose a federated electronic health system based on the clinical document architecture (CDA) that is compliant within the Palestine context. This architecture also brings a normalized electronic health record and a structure of blockchain to enhance interoperability with scalability, fault tolerance, privacy, and security. The new architecture and technologies will enhance services by allowing health care players, patients, and others to have the opportunity to obtain improved access and control of their health services. This may also serve as a useful model for other low-middle income countries.
Cryptic Diversity in the Monotypic Neotropical Micromoth Genus Angelabella (Lepidoptera: Gracillariidae) in the Peru-Chile Desert
Authors: Vargas-Ortiz Marcelo , Aliaga-Pichihua Guido , Lazo-Rivera Ana , Cerdeña José , Farfán Jackie , Huanca-Mamani Wilson , Vargas Héctor A.
Published: October 2020

Angelabella (Lepidoptera: Gracillariidae: Oecophyllembiinae) is considered a monotypic Neotropical genus of leaf miner micromoths known only from a few valleys of the arid environments of the Peru-Chile desert, particularly the southernmost part of Peru and northernmost part of Chile (type locality), where natural populations of its primary host plant occur. The geographic distribution of potential host plants provides a scenario for a wider range for this micromoth genus. The aims of this study were to explore the geographic range of Angelabella, determine the spatial distribution of mitochondrial lineages, and test lineage conspecificity hypotheses. The spatial distribution of genetic diversity indicated the presence of four spatial clusters, three of which are north of the previously known geographic range. Genetic distances were 0.2–0.8% and 3.6–8.3% (K2P) between haplotypes of the same and different spatial clusters, respectively. Phylogenetic relationships indicated reciprocal monophyly among the four spatial clusters, suggesting that allopatric differentiation processes have governed the recent history of Angelabella in these arid environments. These groups were defined as different species by four species delimitation methods, suggesting that Angelabella is not a monotypic genus, but harbors at least four morphologically cryptic allopatric species with restricted geographic ranges, including the type species and three candidate species.
Waste Rubber Pyrolysis: Product Yields and Limonene Concentration
Authors: Januszewicz Katarzyna , Kazimierski Paweł , Suchocki Tomasz , Kardaś Dariusz , Lewandowski Witold , Klugmann-Radziemska Ewa , Łuczak Justyna
Published: October 2020

Tires, conveyor belts, floor mats, and shoe soles form a main-stream of rubber waste. The amount of these used materials continuously increases due to development of the rubber market. Therefore, pro-ecological utilization (i.e., energy recycling instead of burning) and recovering valuable and recyclable materials becomes an urgent necessity. In this regard, this work was devoted to the chemical recycling of selected used rubber products, and it especially explores the possibility of limonene production. Different types of waste rubber were characterized and pyrolyzed at microgram and laboratory scales, and the results were compared. Additionally, the pyrolysis of tires, the most significant stream of rubber waste, was also conducted in a semi-technical scale reactor. The effectiveness of limonene formation in the liquid fractions obtained from different types of waste rubber was compared.
A Review of Toxins from Cnidaria
Authors: D’Ambra Isabella , Lauritano Chiara
Published: October 2020

Cnidarians have been known since ancient times for the painful stings they induce to humans. The effects of the stings range from skin irritation to cardiotoxicity and can result in death of human beings. The noxious effects of cnidarian venoms have stimulated the definition of their composition and their activity. Despite this interest, only a limited number of compounds extracted from cnidarian venoms have been identified and defined in detail. Venoms extracted from Anthozoa are likely the most studied, while venoms from Cubozoa attract research interests due to their lethal effects on humans. The investigation of cnidarian venoms has benefited in very recent times by the application of omics approaches. In this review, we propose an updated synopsis of the toxins identified in the venoms of the main classes of Cnidaria (Hydrozoa, Scyphozoa, Cubozoa, Staurozoa and Anthozoa). We have attempted to consider most of the available information, including a summary of the most recent results from omics and biotechnological studies, with the aim to define the state of the art in the field and provide a background for future research.
Metallurgical Effect of Rare-Earth Lanthanum Fluoride and Boride in the Composite Coating of Wires in the Arc Welding of Bainitic-Martensitic and Austenitic Steel
Authors: Parshin Sergey G.
Published: October 2020

For arc welding of high-strength and cold-resistant steels, the author developed an advanced design of steel wire with a micro-composite coating of a nickel matrix and nanoparticles of LaF3 and LaB6, which improves the metallurgical influence of rare-earth elements (REE) and forms refractory sulphides and oxides of REE, as well as boron nitride. The addition of 0.1–0.3 wt% La in the weld pool leads to an increase in the content of the refractory compounds La2O3, LaO2, and LaS, and to the reduction in the content of the low-melting and brittle oxides and sulphides SiO2, SiO, MnO, MnS, and SiS. The use of steel wire with the composite coating of LaF3 and LaB6 allows for microstructural refinement when welding S960QL bainitic-martensitic steel and X70 API bainitic steel, and increases the impact toughness of the welds by 1.17–1.6 times.
Pre-Treatment Methods for Regeneration of Spent Activated Carbon
Authors: Hwang Sang Youp , Lee Gi Bbum , Kim Ji Hyun , Hong Bum Ui , Park Jung Eun
Published: October 2020

Spent activated carbon (SAC) usually exhibits a low specific surface area due to its high ash contents. In this study, pre-treatments, such as heat and acid treatments, were optimized to improve this feature. The heat pre-treatment did not reduce the ash content, nor did it increase the surface area. Because metallic ions adsorbed in SACs turn into ash upon the heat treatment. In the acid pre-treatment, the volatiles and fixed carbon were increased with decreasing ash contents. In this study, it was found that the surface area increase was correlated with the ratio between fixed carbon and ash. Among the pre-treatment methods, the combined heat and acid pre-treatment method highly increased the ratio, and therefore led to the surface area increase. Additionally, the acid pre-treatment was carried out using different types of acid (organic and inorganic acids) solutions to further improve the surface areas. The organic acid treatment caused a significant structural collapse compared to the inorganic acid treatment, decreasing the surface area. In particular, H3PO4 effectively removed ashes adsorbed on the activated carbon surface and regenerated the exhausted activated carbon. Both the heat and acid pre-treatments before chemical activation resulted in the positive effects such as strong desorption of pollutants and ashes within the internal structure of the activated carbon. Therefore, the regeneration introduced in this study is methodically the best method to regenerate SAC and maintain a stable structure.
Effects of the Daily Consumption of Stevia on Glucose Homeostasis, Body Weight, and Energy Intake: A Randomised Open-Label 12-Week Trial in Healthy Adults
Authors: Stamataki Nikoleta S. , Crooks Benjamin , Ahmed Abubaker , McLaughlin John T.
Published: October 2020

Stevia is a non-nutritive sweetener, providing sweet taste with no calories. This randomised, controlled, open-label 2-parallel arm trial examined the effects of daily stevia consumption on glycaemia in healthy adults. Secondary endpoints included body weight (BW) and energy intake (EI). Healthy participants (n = 28; aged 25 ± 5y, body mass index 21.2 ± 1.7 kg/m2) were randomised into either the stevia group (n = 14)—required to consume a stevia extract daily—or to the control group (n = 14). At weeks 0 and 12, the glucose and insulin responses to an oral glucose tolerance test were measured; BW and EI were assessed at weeks 0, 6, and 12. There was no significant difference in the glucose or insulin responses. There was a significant main effect of group on BW change (F(1,26) = 5.56, p = 0.026), as the stevia group maintained their weight as opposed to the control group (mean weight change at week 12: −0.22 kg, 95%CI [−0.96, 0.51] stevia group, +0.89 kg, 95%CI [0.16, 1.63] control group). The energy intake was significantly decreased between week 0 and 12 in the stevia group (p = 0.003), however no change was found in the control group (p = 0.973). Although not placebo-controlled, these results suggest that daily stevia consumption does not affect glycaemia in healthy individuals, but could aid in weight maintenance and the moderation of EI.
The Role of Human Milk Oligosaccharides and Probiotics on the Neonatal Microbiome and Risk of Necrotizing Enterocolitis: A Narrative Review
Authors: Nolan Lila S. , Rimer Jamie M. , Good Misty
Published: October 2020

Preterm infants are a vulnerable population at risk of intestinal dysbiosis. The newborn microbiome is dominated by Bifidobacterium species, though abnormal microbial colonization can occur by exogenous factors such as mode of delivery, formula feeding, and exposure to antibiotics. Therefore, preterm infants are predisposed to sepsis and necrotizing enterocolitis (NEC), a fatal gastrointestinal disorder, due to an impaired intestinal barrier, immature immunity, and a dysbiotic gut microbiome. Properties of human milk serve as protection in the prevention of NEC. Human milk oligosaccharides (HMOs) and the microbiome of breast milk are immunomodulatory components that provide intestinal homeostasis through regulation of the microbiome and protection of the intestinal barrier. Enteral probiotic supplements have been trialed to evaluate their impact on establishing intestinal homeostasis. Here, we review the protective role of HMOs, probiotics, and synbiotic combinations in protecting a vulnerable population from the pathogenic features associated with necrotizing enterocolitis.
Anti-Obesity Effect of DKB-117 through the Inhibition of Pancreatic Lipase and α-Amylase Activity
Authors: Kim Do Hoon , Park Yu Hwa , Lee Jung Suk , Jeong Hyun Il , Lee Kye Wan , Kang Tong Ho
Published: October 2020

This study sought to evaluate the effects of Phaseolus multiflorus var. albus Bailey extract (PM extract) and Pleurotus eryngii var. ferulae extract (PF extract) on the inhibition of digestive enzymes and to confirm the anti-obesity effect of DKB-117 (a mixture of PM extract and PF extract) in digestive enzyme inhibition in a mouse model of obesity induced by a high-fat diet. In in vitro studies, PM extract and PF extract have increased dose-dependent inhibitory activity on α-amylase (Inhibitory concentration (IC50 value: 6.13 mg/mL)) and pancreatic lipase (IC50 value; 1.68 mg/mL), respectively. High-fat diet–induced obese mice were orally administered DKB-117 extracts at concentrations of 100, 200, and 300 mg/kg/day, while a positive control group was given orlistat (pancreatic lipase inhibitor) and Garcinia cambogia (inhibiting the enzymes needed to synthesize carbohydrates into fat) at concentrations of 40 and 200 mg/kg/day, respectively, for eight weeks. As a result, body weight, fat mass (total fat mass, abdominal fat, and subcutaneous fat) detected with microcomputed tomography, fat mass (abdominal fat and inguinal fat) after an autopsy, and liver triglyceride levels were decreased significantly in the DKB-117 (300 mg/kg/day) group compared to those in the HFD control group. Additionally, we obtained results indicating that the presence of carbohydrates was found more in the DKB-117-300 (300 mg/kg/day) group than in the HFD control group. These data clearly show that DKB-117 extracts are expected to have an anti-obesity effect through a complex mechanism that promotes carbohydrate release through the inhibition of carbohydrate-degrading enzymes while blocking lipid absorption through lipase inhibition.
A Partially Hydrolyzed Whey Infant Formula Supports Appropriate Growth: A Randomized Controlled Non-Inferiority Trial
Authors: Karaglani Eva , Thijs-Verhoeven Inge , Gros Marjan , Chairistanidou Christina , Zervas Giorgos , Filoilia Christina , Kampani Tarek-Michail , Miligkos Vasileios , Matiatou Maria , Valaveri Stavroula , Sakellariou Alexandros , Babilis Georgios , Bos Rolf , Manios Yannis
Published: October 2020

The aim of the current study was to investigate the effects of a partially hydrolyzed whey infant formula (PHF) on growth in healthy term infants as compared to a standard infant formula with intact protein (IPF). In a double-blind, non-inferiority, randomized controlled trial, a total of 163 healthy formula-fed infants, 55–80 days old, were recruited and randomly allocated to either the PHF (test) or the IPF (control) group. They were followed up for three months during which they were evaluated monthly on growth and development. In total, 21 infants discontinued the study, while 142 infants completed the study (test n = 72, control n = 70). The primary outcome was daily weight gain during the three months. Secondary outcomes included additional anthropometric indices at every timepoint over the intervention period. Daily weight gain during the three-month intervention period was similar in both groups with the lower bound of 95% confidence interval (CI) above the non-inferiority margin of −3 g/day [mean difference (95% CI) test vs. control: −0.474 (−2.460, 1.512) g/day]. Regarding secondary outcomes, i.e., infants’ weight, length, head circumference, body mass index (BMI), and their Z-scores, no differences were observed between the two groups at any time point. The PHF resulted in similar infant growth outcomes as the standard IPF. Based on these results, it can be concluded that the partially hydrolyzed whey infant formula supports adequate growth in healthy term infants.
Hypolipidemic Roles of Casein-Derived Peptides by Regulation of Trans-Intestinal Cholesterol Excretion and Bile Acid Synthesis
Authors: Lee Sungmin , Youn BuHyun
Published: October 2020

Hyperlipidemia, a syndrome characterized by an abnormal elevation of blood lipids, causes chronic lethal metabolic disorders. Although statins are regularly prescribed to patients, an alternative to treat the burden of excessive lipids is required for cholesterol control. In this study, it was found that the treatment of casein hydrolyzed by pepsin and trypsin induced trans-intestinal cholesterol excretion (TICE) through ATP-binding cassette subfamily G members 5 (ABCG5) expression. Next, we analyzed sequences of the peptides responsible for TICE induction, synthesized artificial peptides based on the sequences, and the hypolipidemic effects of the peptide treatments were assessed in both in vitro and in vivo models. We determined that two bioactive peptides contained in casein hydrolysates (SQSKVLPVPQK and HPHPHLSF) induced TICE through the expression of ABCG5 in enterocytes and suppressed hepatic mRNA expression of cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and CYP8B1by ileal FGF19 expression both in an liver X receptor α (LXRα)-mediated manner. In the hyperlipidemic mouse models, the oral administration of peptides reduced serum cholesterol levels through elevation of the ABCG5 expression in proximal intestine and fecal cholesterol secretion. Besides this, peptides induced ileal expression of fibroblast growth factor 15/19 (FGF15/19) and inhibited hepatic bile acid synthesis. We found that the oral treatment of casein-derived bioactive peptides could improve hyperlipidemia by regulating intestinal excretion and hepatic synthesis of cholesterols.
Effect of Nitrogen Gas Plasma Generated by a Fast-Pulsed Power Supply Using a Static Induction Thyristor on Scrapie Prion
Authors: Sakudo Akikazu , Imanishi Yuichiro , Hirata Azumi , Koga Yuichi , Shintani Hideharu
Published: October 2020

Previous studies show that nitrogen gas plasma generated by a fast-pulsed power supply using a static induction thyristor has both virucidal and bactericidal effects. In this study, nitrogen gas plasma was further evaluated for its potential effects on prions, which are well known to be the most resistant pathogen to both chemical and physical inactivation. Aliquots (10 μL) of mouse brain homogenate infected with Chandler scrapie prion were spotted onto cover glasses and subjected to nitrogen gas plasma. Treated samples were recovered and subjected to further analyses. Control prion samples were prepared in exactly the same way but without plasma treatment. Protein misfolding cyclic amplification (PMCA) showed that nitrogen gas plasma treatment at 1.5 kilo pulse per second for 15 or 30 min caused a reduction in the in vitro propagation level of PrPres (proteinase K-resistant prion protein), which was used as an index of abnormal prion protein (PrPSc). Moreover, mice injected with prion treated with plasma for 30 min showed longer survival than mice injected with control prion, indicating that nitrogen gas plasma treatment decreased prion infectivity. Altogether, these results suggest that nitrogen gas plasma treatment can inactivate scrapie prions by decreasing the propagation activity and infectivity of PrPSc.