bluuebunny/crossref_metadata_2025 huggingface dataset:
Bioinformatics analysis of myelin-microbe interactions suggests multiple types of molecular mimicry in the pathogenesis of multiple sclerosis
Authors: Bigdeli Ali , Ghaderi-Zefrehei Mostafa , Lesch Bluma J. , Behmanesh Mehrdad , Arab S. Shahriar
Published: December 2024

Multiple sclerosis (MS) is a devastating autoimmune disease that leads to the destruction of the myelin sheath in the human central nervous system (CNS). Infection by viruses and bacteria has been found to be strongly associated with the onset of MS or its severity. We postulated that the immune system’s attack on the myelin sheath could be triggered by viruses and bacteria antigens that resemble myelin sheath components. An in-silico bioinformatics approach was undertaken in order to identify viral and bacterial antigens that resemble myelin oligodendrocyte glycoprotein (MOG) and myelin basic protein (MBP). To this end, we simultaneously analyzed both protein structures and amino acid sequences from viral and bacterial proteins and compared them to MOG and MBP. Possible associations between MBP and human parvovirus B19 (HPV-B19) and adeno-associated virus 4 (AAV-4) capsid protein structures were identified. MBP and MOG were associated with antigens from different viruses and bacteria, including Aspergillus species, Lactobacillus, Burkholderia, Clostridium, Schizosaccharomyces, SARS-CoV-2, and some gut flora metabolites. We also identified similarities between MBP and MOG proteins and bile salt hydrolase (BSH), glycosyltransferase (WcfQ), and Wzy enzymes. Identical amino acids between MBP and BSH at the active site, and protected amino acids in MOG aligning with WcfQ and Wzy enzymes were observed. Overall, our results offer valuable insights into the role of different viral and bacterial protein antigens in MS pathogenesis and suggest the possibility of identifying new therapeutic targets using in silico bioinformatics approaches. Our proposed approach could also likely be adapted for other CNS diseases in order to develop new biological insights and treatments.
The gag-like gene RTL8 antagonizes PEG10-mediated virus like particles
Authors: Campodonico Will , Mohan Harihar M. , Huynh Phuoc T. , Black Holly H. , Lau Cristina I. , Paulson Henry L. , Sharkey Lisa M. , Whiteley Alexandra M.
Published: December 2024

PEG10 is a retroelement-derived Mart-family gene that is necessary for placentation and has been implicated in neurological disease. PEG10 resembles both retrotransposon and retroviral proteins and forms virus-like particles (VLPs) that can be purified using iodixanol ultracentrifugation. It is hypothesized that formation of VLPs is crucial to the biological roles of PEG10 in reproduction and neurological health. Here, we describe the regulation of PEG10 VLP formation and release in human cells with a role for the related Mart gene RTL8. RTL8 resembles a truncated form of PEG10 that shares homology with the N-terminal gag-like capsid domain. Alone, RTL8 is unable to form VLPs, but was incorporated into PEG10-derived particles. RTL8 co-expression decreased the abundance of PEG10 VLPs and increased intracellular levels of PEG10, suggesting a model where RTL8 inhibits PEG10 VLP formation or release. Consistent with this model, RTL8 bound to the N-terminal domain of PEG10 capsid, and modulation of RTL8 influenced PEG10-derived VLP abundance in naturally producing cells. RTL8 is broadly expressed in many of the same tissues as PEG10, including in human brain. Taken together, these results describe a novel antagonistic relationship between two human retroelement-derived genes and have implications for our understanding of PEG10 biology and disease.
Plasmodium falciparum surf4.1 in clinical isolates: From genetic variation and variant diversity to in silico design immunopeptides for vaccine development
Authors: Noranate Nitchakarn , Sripanomphong Jariya , Mphande- Nyasulu Fingani Annie , Chaorattanakawee Suwanna
Published: December 2024

SURFINs protein family expressed on surface of both infected red blood cell and merozoite surface making them as interesting vaccine candidate for erythrocytic stage of malaria infection. In this study, we analyze genetic variation of Pfsurf4.1 gene, copy number variation, and frequency of SURFIN4.1 variants of P. falciparum in clinical isolates. In addition, secondary structure prediction and immunoinformatic were employed to identify immunogenic epitopes in humoral response as proposed vaccine candidates. Overall, our data demonstrate extensive polymorphism of SURFIN4.1 in both genetic and protein level. The surf4.1 gene showed extensive genetic variation with total of 447 polymorphic sites with maximum of three variants as well as singlet/triplet bases indels and mini/microsatellites in the coding sequence. The exon1 encoding extracellular region exhibited higher variation compared to exon2 which coding for intracellular domain. Interestingly, selective pressure was detected on both extracellular region (Var1 and Var2) as well as intracellular region (WRD2 and WRD3). Importantly, extensive full gene analysis suggests adenosine insertion at three key points nucleotide bases (nt 2409/2410, 3809/3810, and 4439/4440) of exon2 could lead to frameshift mutation resulted in four different SURFIN4.1 variants (TMs, WD1, WD2 and WD3). The SURFIN4.1 variant TMs was the most observed type with 67% frequency (51/76). Along with more than one copy number of surf4.1 gene was observed with frequency of 13% (9/70). Despite substantial polymorphism, analysis of relatedness within P. falciparum population using full coding sequence was able to group SURFIN4.1 protein into five distinct clades and reduced into four clades when using only exon1 coding sequence. Also, predicted secondary structure revealed conserved structure of five helix domains of extracellular region which similar among four SURFIN4.1 variant types. In addition, in silico design eight immunopeptides derived from SURFIN4.1, four of which are highly conserved and four of dimorphic epitopes, as potential vaccine candidates.
Utilising causal inference methods to estimate effects and strategise interventions in observational health data
Authors: Duong Bao , Senadeera Manisha , Nguyen Toan , Nichols Melanie , Backholer Kathryn , Allender Steven , Nguyen Thin
Published: December 2024

Randomised controlled trials (RCTs) are the gold standard for evaluating health interventions but often face ethical and practical challenges. When RCTs are not feasible, large observational data sets emerge as a pivotal resource, though these data sets may be subject to bias and unmeasured confounding. Traditional statistical (or non-causal) learning methods, while useful, face limitations in fully uncovering causal effects, i.e., determining if an intervention truly has a direct impact on the outcome. This gap is bridged by the latest advancements in causal inference methods, building upon machine learning-based approaches to investigate not only population-level effects but also the heterogeneous effects of interventions across population subgroups. We demonstrate a causality approach that utilises causal trees and forests, enhanced by weighting mechanisms to adjust for confounding covariates. This method does more than just predict the overall effect of an intervention on the whole population; it also gives a clear picture of how it works differently in various subgroups. Finally, this method excels in strategising and optimising interventions, by suggesting precise and explainable approaches to targeting the intervention, to maximise overall population health outcomes. These capabilities are crucial for health researchers, offering new insights into existing data and assisting in the decision-making process for future interventions. Using observational data from the 2017-18 Australian National Health Survey, our study demonstrates the power of causal trees in estimating the impact of exercise on BMI levels, understanding how this impact varies across subgroups, and assessing the effectiveness of various intervention targeting strategies for enhanced health benefits.
Community pharmacies: Key players in point-of-care diagnostics for STI screening in Africa
Authors: Kiragga Agnes N. , Onzia Annet , Nakate Vivian , Bagaya Irene , Natuha Evelyn , Mande Emmanuel , Kataike Olivia , Parkes-Ratanshi Rosalind , Hamill Matthew M. , Manabe Yukari C.
Published: December 2024

Background

Sexually Transmitted Infections (STIs) rank in the top 5 disease categories for which adults in developing countries seek healthcare services. Community pharmacies offer clients convenience, proximity, extended opening hours, privacy, and efficiency, which could make them desirable locations for HIV and STI screening and treatment. We examined the feasibility of using point-of-care (POC) STI tests for screening HIV and other STIs at community pharmacies.

Methods

We conducted a prospective cohort study of persons seeking medication and other services at 18 purposively selected community pharmacies in Kampala, Uganda. Study participants comprised two broad categories: i) Symptomatic persons aged 18 years who presented with at least one STI sign or symptom and were purchasing treatment for themselves; ii) persons presenting with no STI symptom who had come to purchase any other medication, including family planning services such as emergency contraception. POC tests were used to test HIV, Chlamydia trachomatis (Ct), Neisseria gonorrhoeae (Ng), Trichomonas vaginalis (Tv), and Syphilis. Test results were returned on-site or via telephone within 48 to 72 hours. Descriptive statistics were used to estimate the prevalence of STIs.

Results

Of the 450 participants enrolled, 235 (52.2%) were symptomatic, 215 (47.8%) were asymptomatic, and 280 (62.2%) were females. STI testing was feasible, with an acceptability rate of 99.8%. 135 (30%) of participants had at least one STI; HIV prevalence was 39 (8.7%), Syphilis prevalence was 14 (3.1%), 50 (11.1%) tested positive for Ng, 39 (8.7%) were positive for Ct while. The prevalence of Tv was 25 (8.9%) (tested among women). A total of 107 (23.8%) participants had used an antibiotic in the preceding month.

Conclusion

Our research underscores the potentially pivotal role of community pharmacies in deploying POC diagnostics for STIs and antimicrobial stewardship by decreasing unnecessary antibiotic dispensation across Africa.
Artificial intelligence applied to bed regulation in Rio Grande do Norte: Data analysis and application of machine learning on the “RegulaRN Leitos Gerais” platform
Authors: Barreto Tiago de Oliveira , Farias Fernando Lucas de Oliveira , Veras Nicolas Vinícius Rodrigues , Cardoso Pablo Holanda , Silva Gleyson José Pinheiro Caldeira , Pinheiro Chander de Oliveira , Medina Maria Valéria Bezerra , Fernandes Felipe Ricardo dos Santos , Barbalho Ingridy Marina Pierre , Cortez Lyane Ramalho , Santos João Paulo Queiroz dos , Morais Antonio Higor Freire de , Souza Gustavo Fontoura de , Machado Guilherme Medeiros , Lucena Márcia Jacyntha Nunes Rodrigues , Valentim Ricardo Alexsandro de Medeiros
Published: December 2024

Bed regulation within Brazil’s National Health System (SUS) plays a crucial role in managing care for patients in need of hospitalization. In Rio Grande do Norte, Brazil, the RegulaRN Leitos Gerais platform was the information system developed to register requests for bed regulation for COVID-19 cases. However, the platform was expanded to cover a range of diseases that require hospitalization. This study explored different machine learning models in the RegulaRN database, from October 2021 to January 2024, totaling 47,056 regulations. From the data obtained, 12 features were selected from the 24 available. After that, blank and inconclusive data were removed, as well as the outcomes that had values other than discharge and death, rendering a binary classification. Data was also correlated, balanced, and divided into training and test portions for application in machine learning models. The results showed better accuracy (87.77%) and recall (87.77%) for the XGBoost model, and higher precision (87.85%) and F1-Score (87.56%) for the Random Forest and Gradient Boosting models, respectively. As for Specificity (82.94%) and ROC-AUC (82.13%), the Multilayer Perceptron with SGD optimizer obtained the highest scores. The results evidenced which models could adequately assist medical regulators during the decision-making process for bed regulation, enabling even more effective regulation and, consequently, greater availability of beds and a decrease in waiting time for patients.
Comparing subsampling strategies for metagenomic analysis in microbial studies using amplicon sequence variants versus operational taxonomic units
Authors: Segura Daniel , Sharma Divya , Espin-Garcia Osvaldo
Published: December 2024

The microbiome is increasingly regarded as a key component of human health, and analysis of microbiome data can aid in the development of precision medicine. Due to the high cost of shotgun metagenomic sequencing (SM-seq), microbiome analyses can be done cost-effectively in two phases: Phase 1-sequencing of 16S ribosomal RNA, and Phase 2-SM-seq of an informative subsample. Existing research suggests strategies to select the subsample based on biological diversity and dissimilarity metrics calculated using operational taxonomic units (OTUs). However, the microbiome field has progressed towards amplicon sequencing variants (ASVs), as they provide more precise microbe identification and sample diversity information. The aim of this work is to compare the subsampling strategies for two-phase metagenomic studies when using ASVs instead of OTUs, and to propose data driven strategies for subsample selection through dimension reduction techniques. We used 199 samples of infant-gut microbiome data from the DIABIMMUNE project to generate ASVs and OTUs, then generated subsamples based on five existing biologically driven subsampling methods and two data driven methods. Linear discriminant analysis Effect Size (LEfSe) was used to assess differential representation of taxa between the subsamples and the overall sample. The use of ASVs showed a 50-93% agreement in the subsample selection with the use of OTUs for the subsampling methods evaluated, and showed a similar bacterial representation across all methods. Although sampling using ASVs and OTUs typically lead to similar results for each subsample, ASVs had more clades that differed in expression levels between allergic and non-allergic individuals across all sample sizes compared to OTUs, and led to more biomarkers discovered at Phase 2-SM-seq level.
Fault diagnosis of nonlinear analog circuits using generalized frequency response function and LSSVM
Authors: Zhang Jialiang , Yang Yaowang
Published: December 2024

A fault diagnosis method of nonlinear analog circuits is proposed that combines the generalized frequency response function (GFRF) and the simplified least squares support vector machine (LSSVM). In this study, the harmonic signal is used as an input to estimate the GFRFs. To improve the estimation accuracy, the GFRFs of an analog circuit are solved directly using time-domain data. The Fourier transform of the time-domain data is avoided. After obtaining the fault features, a multi-fault classifier is designed based on the LSSVM. In order to improve the training speed and reduces storage, a simplified LSSVM model is used to construct the classifier, and the conjugate gradient algorithm is used for training. The fault diagnosis simulation experiment is conducted on a biquad filter circuit to verify the proposed method. The experimental results show that the proposed method has high diagnostic accuracy and short training time.
Behavioral choice of manufacturers, recyclers and customers in Trade-In Programs
Authors: He Chan , An Qianru
Published: December 2024

This study investigates the behavioral choices of manufacturers, recyclers, and customers in Trade-In Programs designed to promote recycling and environmental sustainability. Using a manufacturer-led evolutionary game model, the research explores how factors such as government policies, market demand, financial incentives, and the simplicity of participation impact stakeholder engagement in these programs. Numerical simulations were conducted to analyze the effects of specific parameters on each participants’ willingness to participate. Results show that increased subsidies and investment in innovation by manufacturers significantly enhance recycler and customer participation in ethic Trade-In Programs. Conversely, higher revenue-sharing by manufacturers tends to reduce engagement from both parties, while manufacturers receiving substantial brand and social benefits alongside government subsidies to encourage broader stakeholder involvement. These findings offer critical insights for designing effective Trade-In Programs, supporting sustainable recycling practices and circular economy principles. The study provides actionable guidance for policymakers and industry leaders aiming to boost participation in environmentally focused recycling initiatives, laying a foundation for more sustainable industrial practices.
Dual harm among patients in the Ontario forensic mental health system
Authors: Kaggwa Mark Mohan , Erb Bailea , Prat Sébastien , Davids Arianna , Bradford John , Chaimowitz Gary Andrew , Olagunju Andrew Toyin
Published: December 2024

Background

Dual harm involves the unfortunate experience of harm to self and others/objects. Safeguarding individuals in forensic psychiatric settings against all forms of harm to self and others is sacrosanct. While understanding dual harm is crucial in the care and rehabilitation of patients in forensic psychiatric settings, only a few studies have explored this phenomenon. This study examined dual harm and its associated clinical and sociodemographic factors among forensic patients in Ontario, Canada.

Methods

In this retrospective study, we used data from the Ontario Review Board (ORB) (n = 1240; mean age 42.54±3.32 years, and 85.73% male). We defined dual harm as the co-occurrence of self-harming behaviour in the last 12 months and violent behaviour towards others or objects (such as verbal, physical, or sexual aggression). We analysed the data in relation to clinical and sociodemographic factors.

Results

Of 1240 patients, 43 (3.55%) had engaged in dual harm. Most of them had engaged in dual harm related to verbal aggression (3.15%), followed by dual harm related to aggression towards objects (2.97%), dual harm related to aggression towards others (2.73%), and dual harm related to sexual aggression was the rarest (1.32%). Only 12 patients had engaged in all types of dual harm. Having a previous history of dual harm and a diagnosis of a neurodevelopmental disorder increased the chance of perpetrating/engaging in dual harm. However, increasing age and a higher education decreased the chance of dual harm. These factors were similar for different types of dual harm, except for dual harm related to sexual aggression, which was the only subtype associated with having a personality disorder diagnosis. Again, the experience of violence increased the chance of self-harm.

Conclusion

Dual harm is present among forensic patients in Ontario, and self-harm is prevalent among individuals with various forms of aggression, especially when the violence was perpetrated towards objects. Strategies to manage the risk of self-harm among aggressive patients should be put in place to mitigate dual harm and the associated complications, especially among individuals with neurodevelopmental disorders.
Assessing the value of deep neural networks for postoperative complication prediction in pancreaticoduodenectomy patients
Authors: Bonde Mikkel , Bonde Alexander , Kaafarani Haytham , Millarch Andreas , Sillesen Martin
Published: December 2024

Introduction

Pancreaticoduodenectomy (PD) for patients with pancreatic ductal adenocarcinoma (PDAC) is associated with a high risk of postoperative complications (PoCs) and risk prediction of these is therefore critical for optimal treatment planning. We hypothesize that novel deep learning network approaches through transfer learning may be superior to legacy approaches for PoC risk prediction in the PDAC surgical setting.

Methods

Data from the US National Surgical Quality Improvement Program (NSQIP) 2002–2018 were used, with a total of 5,881,881 million patients, including 31,728 PD patients. Modelling approaches comprised of a model trained on a general surgery patient cohort and then tested on a PD specific cohort (general model), a transfer learning model trained on the general surgery patients with subsequent transfer and retraining on a PD-specific patient cohort (transfer learning model), a model trained and tested exclusively on the PD-specific patient cohort (direct model), and a benchmark random forest model trained on the PD patient cohort (RF model). The models were subsequently compared against the American College of Surgeons (ACS) surgical risk calculator (SRC) in terms of predicting mortality and morbidity risk.

Results

Both the general model and transfer learning model outperformed the RF model in 14 and 16 out of 19 prediction tasks, respectively. Additionally, both models outperformed the direct model on 17 out of the 19 tasks. The transfer learning model also outperformed the general model on 11 out of the 19 prediction tasks. The transfer learning model outperformed the ACS-SRC regarding mortality and all the models outperformed the ACS-SRC regarding the morbidity prediction with the general model achieving the highest Receiver Operator Area Under the Curve (ROC-AUC) of 0.668 compared to the 0.524 of the ACS SRC.

Conclusion

DNNs deployed using a transfer learning approach may be of value for PoC risk prediction in the PD setting.
SH2D5 promotes lung adenocarcinoma cell metastasis and triggers EMT via activating AKT signaling pathway
Authors: Du Licheng , Ren Wenjia , Liu Linjun , Zhu Haojia , Xu Ke , Zhou Yubai
Published: December 2024

Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer, characterized by a high incidence in late stages, high mortality rate, and poor prognosis. Src Homology 2 Domain Containing Protein 5 (SH2D5) is a mammalian-specific, uncharacterized scaffolding protein, and its role in LUAD remains unclear. In the present study, we investigated the function and potential mechanisms of SH2D5 in the progression of LUAD. We found aberrant expression of SH2D5 in LUAD tissues and cells, and its high expression is closely associated with poor prognosis in LUAD patients. Through loss-of-function and gain-of-function experiments, we revealed that overexpression of SH2D5 promotes the proliferation and migration abilities of lung adenocarcinoma cells. Gene set enrichment analysis (GSEA) revealed that SH2D5 positively regulates the epithelial-mesenchymal transition (EMT) process in lung adenocarcinoma cells. Additionally, we found that regulating the expression of SH2D5 influenced the phosphorylation levels of AKT, and the rescue experiments with AKT pathway activators/inhibitors partially reversed the tumor progression and EMT processes induced by SH2D5. In summary, our study demonstrated that SH2D5 promotes the migration and EMT process of LUAD cells through the AKT signaling pathway, suggesting that SH2D5 may serve as a crucial potential target for the treatment of metastatic LUAD.
Measurement properties of the sit-to-stand test in people with chronic obstructive pulmonary disease: Protocol for a systematic review and meta-analysis using the COSMIN guidelines
Authors: Farley Christopher , Phillips Stuart M. , Smith-Turchyn Jenna , Brooks Dina
Published: December 2024

Introduction

Characteristics of chronic obstructive pulmonary disease (COPD) can include shortness of breath, chronic cough, sputum production and reduced exercise capacity. The sit-to-stand (STS) test variations (e.g., 5-repetition STS, 30-second STS) may be appropriate outcome measures to assess exercise capacity in people with COPD. To date, the measurement properties of the various STS tests in people with COPD have not been synthesized in a systematic review since the publication of the COnsensus‐based Standards for the selection of health Measurement Instruments (COSMIN) guidelines. The purpose of this proposed systematic review is to synthesize the literature of the measurement properties of the STS test variations among people with COPD.

Materials and methods

The review will be conducted with methods consistent with the COSMIN guidelines. Peer-reviewed publications will be included if they assessed the measurement properties (reliability, validity, responsiveness) of a STS test in community-dwelling adults with COPD. We will search six databases from inception. Study selection and data extraction will be conducted independently and in duplicate. We will assess the risk of bias using the COSMIN Risk of Bias tool, assess results against the COSMIN updated criteria for good measurement properties, and summarize certainty of evidence using the modified Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool. Study results will be pooled by calculating weighted means and 95% confidence intervals or summarized narratively, as appropriate.

Conclusion

This will be the first systematic review to synthesize the measurement properties of the STS tests in people with COPD using the methods recommended by COSMIN. Consequently, its results will be robust and may help clinicians or researchers identify the best variation of the STS test to use in their practice.
Integrative use of DNA barcode and morphology reveal high level of diversity in the ornamental fish on the lower Amazon basin
Authors: de Freitas Elciane Araújo , dos Santos Dayana Batista , Moraes Ferreira Charles Samuel , Silva-Oliveira Cárlison , Evangelista-Gomes Grazielle Fernanda , Veneza Ivana Barbosa
Published: December 2024

The Amazon basin is the world’s largest hydrographic basin, in terms of both its total area and its species diversity, with more than 2,700 species of fish. Despite this diversity, the data available on the fish fauna of the Amazon basin are still relatively scant and incomplete, in particular from the streams and floodplain lakes of the lower Amazon, which may contain a large proportion of the still undescribed species of the basin. Many of these species are expected to be of interest to the ornamental fish market. The investigation of the diversity of potential ornamental fish using molecular tools is even more limited. Given this scenario, the present study employed DNA barcoding to investigate the diversity of ornamental fish found in two streams and a floodplain lake of the lower Amazon. The mitochondrially encoded cytochrome c oxidase I (MT-CO1) molecular marker was used to identify the taxa, in combination with morphological keys. A total of 51 ornamental species were identified, representing 13 families and three orders. A majority of the species were found at only one of the sampling points, which indicates that the distribution of the species is influenced by ecological factors. The most speciose order was the Characiformes, followed by the Cichliformes and Siluriformes, while the family with the greatest diversity of species was the Acestrorhamphidae (31.3% of the total number of species), followed by the Cichlidae (27.4%), and the Lebiasinidae (9.8%). One specie was registered in the region of the lower Amazon for the first time, and evidence was found of the possible existence of species not formally described of Aphyocharax, Astyanax, Apareiodon and Hemigrammus.
Identification of markers correlating with mitochondrial function in myocardial infarction by bioinformatics
Authors: Kuang Wenlong , Huang Jianwu , Yang Yulu , Liao Yuhua , Zhou Zihua , Liu Qian , Wu Hailang
Published: December 2024

Background

Myocardial infarction (MI), one of the most serious cardiovascular diseases, is also affected by altered mitochondrial metabolism and immune status, but their crosstalk is poorly understood. In this paper, we use bioinformatics to explore key targets associated with mitochondrial metabolic function in MI.

Methods

The datasets (GSE775, GSE183272 and GSE236374) were from National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) in conjunction with mitochondrial gene data that were downloaded from the MitoCarta 3.0 database. Differentially expressed genes (DEGs) in the dataset were screened by ClusterGVis, Weighted Gene Co-Expression Network Analysis (WGCNA) and GEO2R, and functional enrichment was performed by Gene Set Enrichment Analysis (GSEA) and Kyoto Encyclopedia of Genomes (KEGG). Then mitochondria-associated DEGs (MitoDEGs) were obtained. Protein-protein interaction (PPI) networks were constructed to identify central MitoDEGs that are strongly associated with MI. The Cytoscape and miRWalk databases were then used to predict the transcription factors and target miRNAs of the central MitoDEG, respectively. Finally, the mouse model has been established to demonstrate the expression of MitoDEGs and their association with cardiac function.

Results

MitoDEGs in MI were mainly involved in mitochondrial function and adenosine triphosphate (ATP) synthesis pathways. The 10 MI-related hub MitoDEGs were then obtained by eight different algorithms. Immunoassays showed a significant increase in monocyte macrophage and T cell infiltration. According to animal experiments, the expression trends of the four hub MitoDEGs (Aco2, Atp5a1, Ndufs3, and Ndufv1) were verified to be consistent with the bioinformatics results.

Conclusion

Our study identified key genes (Aco2, Atp5a1, Ndufs3, and Ndufv1) associated with mitochondrial function in myocardial infarction.
The proteomic content of Varroa destructor gut varies according to the developmental stage of its host
Authors: Piou Vincent , Arafah Karim , Bocquet Michel , Bulet Philippe , Vétillard Angélique
Published: December 2024

The nutritional physiology of parasites is often overlooked although it is at the basis of host-parasite interactions. In the case of Varroa destructor, one of the major pests of the Western honey bee Apis mellifera, the nature of molecules and tissues ingested by the parasite is still not completely understood. Here, the V. destructor feeding biology was explored through artificial feeding, dissection of the mite’s gut and proteomic analyses. More specifically, the proteome of guts extracted from starved mites and honey bee-fed mites was compared to highlight both the parasite proteins likely involved in food processing and the honey bee proteins actually ingested by the mite. We could identify 25 V. destructor candidate proteins likely involved in the parasite digestion. As the host developmental stages infested by the mite are diverse, we also focused on the identity and on the origin of honey bee proteins ingested by the mite when it feeds on larvae, pupae or adults. We highlighted profiles of consumed honey bee proteins and their variations throughout the V. destructor life cycle. These variations matched the ones observed in the honey bee hemolymph, showing that this tissue is an important part of the mite’s diet. Based on the variations of abundance of the most consumed honey bee proteins and on their functions, the potential implication of these key candidate nutrients in V. destructor reproduction is also discussed.
Withaferin A inhibits Chikungunya virus nsP2 protease and shows antiviral activity in the cell culture and mouse model of virus infection
Authors: Sharma Kiran Bala , Subramani Chandru , Ganesh Khashpatika , Sharma Anshula , Basu Brohmomoy , Balyan Shivani , Sharma Ghanshyam , KA Shouri , Deb Arundhati , Srivastava Mitul , Chugh Saurabh , Sehrawat Sapna , Bharadwaj Kanchan , Rout Archana , Sahoo Pankaj Kumar , Saurav Suman , Motiani Rajender K. , Singh Ramandeep , Jain Deepti , Asthana Shailendra , Wadhwa Renu , Vrati Sudhanshu
Published: December 2024

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus causing fever, myalgia, and debilitating joint swelling and pain, which in many patients becomes chronic. The frequent epidemics of CHIKV across the world pose a significant public health burden necessitating the development of effective antiviral therapeutics. A cellular imaging-based high-content screening of natural compounds identified withaferin A (WFA), a steroidal lactone isolated from the plant Withania somnifera, as a potent antiviral against CHIKV. In the ERMS cells, WFA inhibited CHIKV replication early during the life cycle by binding the CHIKV non-structural protein nsP2 and inhibiting its protease activity. This inhibited the viral polyprotein processing and the minus-sense viral RNA synthesis. WFA mounted the nsP2 protease inhibitory activity through its oxidising property as the reducing agents N-acetylcysteine and Glutathione-monoethyl ester effectively reversed the WFA-mediated protease inhibition in vitro and abolished the WFA-mediated antiviral activity in cultured cells. WFA inhibited CHIKV replication in the C57BL/6 mouse model of chikungunya disease, resulting in significantly lower viremia. Importantly, CHIKV-infected mice showed significant joint swelling which was not seen in WFA-treated mice. These data demonstrate the potential of WFA as a novel CHIKV antiviral.
National Trends in Lumbar Degenerative Spondylolisthesis With Stenosis Treated With Fusion Versus Decompression
Authors: Ball Jacob R. , Gallo Matthew C. , Kebaish Kareem , Hang Nicole , Ton Andy , Hernandez Fergui , Abdou Marc , Karakash William J. , Wang Jeffrey C. , Hah Raymond J. , Alluri Ram K.
Published: December 2024

Objective: The purpose of this study is to describe utilization, demographics, complications, and revisions for patients with degenerative spondylolisthesis (DS) with stenosis undergoing decompression or decompression with fusion in the United States.Methods: A national insurance database was used to identify patients who underwent either decompression and fusion or decompression alone for management of DS from 2010–2022. Utilization trends, demographics, and complications for each procedure were compared.Results: A total of 162,878 patients were identified, of which 78,043 patients underwent combined single-level lumbar decompression and fusion and 84,835 underwent single-level lumbar decompression alone. Between 2010–2021, lumbar decompression and fusion became the predominant surgical intervention for DS in 2016 and continued to account for more than half of all procedures during the remainder of the study period. Factors such as age, sex, comorbidities, geographic region, and physician specialty training were associated with procedure choice. Decompression with fusion was associated with a lower risk of revision surgery up to 5 years postoperatively and an overall lower incidence of 30-day complications.Conclusion: Decompression with fusion has become the most common treatment for lumbar DS over the past decade despite a lack of compelling evidence supporting its use compared to decompression alone. A variety of patient and surgeon-specific factors is associated with procedure choice. After accounting for cofounders, we identified treatment-specific complications that may be valuable when counseling patients.
Transforaminal Lumbar Endoscopic Discectomy: A Novel Alternative for Management of Lumbar Disc Herniation in Patients With Rheumatoid Arthritis?
Authors: Kapetanakis Stylianos , Chaniotakis Constantinos , Tsioulas Paschalis , Gkantsinikoudis Nikolaos
Published: December 2024

Objective: Lumbar disc herniation (LDH) represents an increasingly encountered condition in patients with rheumatoid arthritis (RA). The aim of the present study is to assess the progress of health-related quality of life following transforaminal endoscopic lumbar discectomy (TELD) for LDH in patients suffering from RA.Methods: Seventy-four patients, scheduled to undergo elective TELD for LDH, were prospectively enrolled in the study. Group A included 36 otherwise healthy individuals and group B 38 patients complementarily diagnosed with RA according to the 2010 ACR/EULAR (American College of Rheumatology/European League Against Rheumatism) criteria. The Medical Outcomes Study 36-item Short Form health survey (SF-36) was selected for the outcome assessment at baseline and postoperatively, at selected intervals at 6 weeks, 3, 6, and 12 months postoperatively.Results: Group A presented statistically significantly higher scores in all SF-36 domains and all selected intervals (p<0.001), except for mental health parameter. All aspects of SF-36 questionnaire significantly improved postoperatively (p<0.001) and in each group independently. Nevertheless, the absolute improvement between consecutive time intervals did not differ significantly between the 2 groups.Conclusion: Patients diagnosed with RA who undergo TELD for LDH demonstrate statistically significant improvement in their health status, as measured by SF-36 questionnaire, one year after the procedure. This improvement is comparable with normal individuals.
Safety Profile of Biportal Endoscopic Spine Surgery Compared to Conventional Microscopic Approach: A Pooled Analysis of 2 Randomized Controlled Trials
Authors: Park Sang-Min , Song Kwang-Sup , Ham Dae-Woong , Kim Ho-Joong , Kang Min-Seok , You Ki-Han , Park Choon Keun , Lee Dong-Keun , Kim Jin-Sung , Lee Hong-Jae , Park Hyun-Jin
Published: December 2024

Objective: To compare the safety profiles of biportal endoscopic spinal surgery (BESS) and microscopic spinal surgery (MSS) for lumbar disc herniation and spinal stenosis by analyzing the associated adverse events.Methods: We pooled data from 2 prospective randomized controlled trials involving 220 patients (110 in each group) who underwent single-level lumbar surgery. Participants aged 20–80 years with radiating pain due to lumbar disc herniation or spinal stenosis were included in this study. Adverse events were recorded and analyzed over a 12-month follow-up period.Results: The overall adverse event rates were 9.1% (10 of 110) in the BESS group and 17.3% (19 of 110) in the MSS group, which were not statistically significantly different (p=0.133). Notably, wound dehiscence occurred in 8.2% of MSS cases but in none of the BESS cases. Both groups showed similarly low rates of complications, such as dural tears, epidural hematoma, and nerve root injury. The most common adverse event in the BESS group was recurrent disc herniation (2.7%), whereas that in the MSS group was wound dehiscence (8.2%).Conclusion: BESS demonstrated a safety profile comparable to that of MSS for the treatment of lumbar disc herniation and spinal stenosis, with a trend towards fewer overall complications. BESS offers particular advantages in terms of reducing wound-related complications. These findings suggest that BESS is a safe alternative to conventional MSS and potentially offers the benefits of a minimally invasive approach without compromising patient safety.
Endoscopic Contralateral Transaxillary Discectomy for Recurrent Disc Herniation
Authors: Gollogly Sohrab , Yue James , Van Isseldyk Facundo , Kim Jin-Sung , Farshad Mazda
Published: December 2024

This video aims to describe an endoscopic surgical approach for accessing difficult to reach pathology such as disc herniations after previous surgery. The relatively small size of endoscopic instruments facilitates significant freedom of movement inside the spinal canal. The authors have experience with interlaminar approaches for contralateral pathology such as disc herniations, recurrent disc herniations, spinal stenosis, and facet cysts. The advantages of starting from the opposite side of the canal in a revision situation include the ability to establish a clear plane between the dura and the borders of the canal and visualize the disc from a different angle than the index operation. Contralateral approaches to residual or recurrent herniations can be performed with an “over the top” technique, navigating dorsal to the thecal sac to reach the far side of the canal. In the associated video we demonstrate a novel technique, a contralateral transaxillary endoscopic approach to a recurrent disc herniation at the L5–S1 level in a young male collegiate wrestler. In our experience, we have found this particular approach to be useful in patients with an early take off of the S1 nerve root which creates a large axillary window. In several instances this technique has allowed us to inspect the area of the reherniation from both the axilla and over the top of the thecal sac. This particular patient has a large recurrence 2 years after an open microscopic hemilaminotomy and discectomy. In this instance, an approach was chosen that navigates dorsal to the S1 nerve root and ventral to the thecal sac, starting on the opposite side of the spinal canal from the herniation. This approach is described as a contralateral interlaminar transaxillary discectomy.
Hybrid Endoscopic Thoracic Discectomy Using Robotic Arm and Navigation for Highly Migrated Calcified Disc Herniation
Authors: Liang Yi-Hao , Kavishwar Rohit Akshay , Pedraza Maria , Setiawan Dimas Rahman , Kim Jae-Hwan , Kim Jin-Sung
Published: December 2024

This video provides a step-by-step guide for performing the hybrid endoscopic thoracic discectomy using navigation and robotic arm for addressing high migrated calcified disc herniation. With the development of techniques, endoscopic spine surgery has emerged as a reliable treatment for thoracic myelopathy. This approach offers high-resolution, off-axis visualization of the surgical field. The field is poised to advance further as endoscopic instruments are refined, becoming less invasive and more precise through the integration of navigation and robot-assisted systems. A 62-year-old woman presented to us with chief complaints of both legs weakness. She had difficulty standing and walking after squatted due to weakness in her legs and her Oswestry Disability Index score was 66. On examination her both side knee extension and ankle dorsiflexion were grade 4 without dysesthesia. The imaging examination confirmed the diagnosis of thoracic myelopathy caused by a highly migrated calcified disc herniation at T5–6 level. The patient underwent an endoscopic thoracic discectomy using robotic arm and navigation for addressing highly migrated calcified disc herniation, resulting in an excellent outcome. The continuous development of navigation and robotic systems in endoscopic thoracic surgery enhanced accuracy in surgical incisions and instrument placement, as well as improved efficiency in locating pathology and achieving precise decompression. Endoscopic thoracic discectomy combines full-endoscopy and unilateral biportal endoscopic (UBE) techniques to leverage the benefits of both approaches, including the cross-viewing of full-endoscopy cannula and the use of larger Kerrison rongeurs under UBE.
Paramedian Unilateral ‘Bitubular’ Endoscopic Access for a Far Lateral Disc Herniation: A Novel Approach for Far Lateral Lumbar Pathologies
Authors: Kerr Hui-Ling , Wadikhaye Rohit , Sun Gyeong Ung , Park Cheol Woong
Published: December 2024

We present a novel technique to approach far lateral lumbar pathologies using a bitubular, biportal endoscopic system and a paramedian approach. Background: Conventional approaches for lumbar far lateral discectomy range from open approaches to newer minimally invasive approaches such as tubular discectomy and single portal endoscopic discectomy. We present a case of a patient suffering with a left L3–4 and left L4–5 extraforaminal disc herniation who was treated successfully with a left sided bitubular, biportal endoscopic 2 level far lateral discectomy. A paramedian ‘bitubular’ biportal endoscopic approach is safe and effective for far lateral lumbar pathologies with excellent visualisation due to good outflow of irrigation fluid.
Full-Endoscopic J-Shaped Transforaminal L5 Nerve Decompression in Bertolotti Syndrome
Authors: Ju Chang-Il , Kim Pius , Seo Jong Hun
Published: December 2024

This case report and video demonstrate the technique of full-endoscopic J-shaped transforaminal L5 exiting nerve decompression in Bertolotti syndrome. Bertolotti syndrome, characterized by a congenital lumbosacral transitional vertebra, often results in mechanical lower back pain and nerve root compression. A 69-year-old male presented with progressive radiating pain in the right leg and tingling in the L5 dermatome. Lumbar spine MRI revealed a right foraminal disc herniation at the L5–S1 level, with calcification and foraminal stenosis. The patient was also diagnosed with Castellvi type I Bertolotti syndrome, featuring a large L5 transverse process and a high iliac crest. These anatomical variations complicated the transforaminal approach, creating a narrow safety zone for conventional methods. The approach began with docking on the L5 transverse process. Endoscopic drilling was performed in a J-shaped configuration to partially resect the transverse process and alar wing, facilitating endoscope insertion into Kambin’s triangle. Foraminal decompression was achieved by removing the tip of the superior articular process (SAP), thereby decompressing the L5 exiting nerve root. Full-endoscopic spine surgery offers a safe and effective alternative to traditional open techniques for L5 nerve decompression in Bertolotti syndrome. This video presentation illustrates the intraoperative endoscopic approach, detailing the decompression techniques and highlighting the minimally invasive advantages of this method.
O-Arm Navigation-Guided Unilateral Biportal Endoscopic Decompression of Far-Out Syndrome
Authors: Kavishwar Rohit Akshay , Liang YiHao , Lee Dongeon , Kim Jaehwan , Pedraza Maria , Kim Jin-Sung
Published: December 2024

The main aim of this video article is to demonstrate the combined use of O-arm navigation and unilateral biportal endoscopy (UBE) to manage far-out syndrome (FOS). In FOS there is entrapment and compression of the fifth lumbar nerve beyond the foramen and between L5 transverse process and the sacral ala at the lumbosacral junction. Conventional microscopic decompression using a paraspinal approach had been the gold standard for its management. However, the surgery is technically challenging due to the deep location of the pathology and intricate anatomy of extraforaminal space. There have been some published reports of unsatisfactory outcomes with microscopic decompression for FOS. We decided to integrate navigation with UBE to increase precision for the management of FOS. A 70-year-old female presented to us with chief complaint of left lower limb radiculopathy since 1 year. She also complained of numbness and paresthesias in her left leg and foot. She was unable to walk for more than 10 minutes due to pain. Her magnetic resonance imaging scan revealed compression of left L5 nerve root in the extraforaminal region. UBE decompression via paraspinal approach was performed for her under O-arm navigation. She experienced immediate relief of her symptoms in the postoperative period. O-arm-navigation-guided UBE is an effective and safer alternative to microsurgical decompression for the management of FOS. This video demonstrates the step-by-step implementation of O-arm navigation with endoscopy and its precise execution.