bluuebunny/crossref_metadata_2025 huggingface dataset:
Invasion Genetics of the Horse-Chestnut Leaf Miner, Cameraria ohridella (Lepidoptera: Gracillariidae), in European Russia: A Case of Successful Involvement of Citizen Science in Studying an Alien Insect Pest
Authors: Kirichenko Natalia I. , Karpun Natalia N. , Zhuravleva Elena N. , Shoshina Elena I. , Anikin Vasily V. , Musolin Dmitrii L.
Published: January 2023

Based on the intensive monitoring conducted by our team and volunteers in 2021, the secondary range of an alien horse-chestnut leaf miner, Cameraria ohridella Deschka & Dimić, 1986 (Lepidoptera: Gracillariidae), was specified in European Russia. This invasive pest was confirmed in 24 out of 58 administrative regions of Russia, which it has occupied for approximately 16 years. Analysis of the COI mtDNA gene sequenced in 201 specimens collected in 21 regions of the European part of Russia indicates the occurrence of two haplotypes (A and B), which are also present in the secondary range of C. ohridella in Eastern and Western Europe. The haplotype A dominated and was present in 87.5% of specimens from European Russia. In 2021, C. ohridella produced spectacular outbreaks in Aesculus hippocastanum in southern Russia, where it damaged more than 50% of the leaves in trees in 24 out of 30 distant localities. In the south of the country, the pest infested Acer pseudoplatanus, whereas other species of Acer of European, East Asian, and North American origin showed no signs of attacks. Taking into account that Ae. hippocastanum is present in most regions of European Russia, we expect a further range expansion of C. ohridella up to the Ural Mountains.
Best Practices for Comprehensive Annotation of Neuropeptides of Gryllus bimaculatus
Authors: Mochizuki Takako , Sakamoto Mika , Tanizawa Yasuhiro , Seike Hitomi , Zhu Zhen , Zhou Yi Jun , Fukumura Keisuke , Nagata Shinji , Nakamura Yasukazu
Published: January 2023

Genome annotation is critically important data that can support research. Draft genome annotations cover representative genes; however, they often do not include genes that are expressed only in limited tissues and stages, or genes with low expression levels. Neuropeptides are responsible for regulation of various physiological and biological processes. A recent study disclosed the genome draft of the two-spotted cricket Gryllus bimaculatus, which was utilized to understand the intriguing physiology and biology of crickets. Thus far, only two of the nine reported neuropeptides in G. bimaculatus were annotated in the draft genome. Even though de novo assembly using transcriptomic analyses can comprehensively identify neuropeptides, this method does not follow those annotations on the genome locus. In this study, we performed the annotations based on the reference mapping, de novo transcriptome assembly, and manual curation. Consequently, we identified 41 neuropeptides out of 43 neuropeptides, which were reported in the insects. Further, 32 of the identified neuropeptides on the genomic loci in G. bimaculatus were annotated. The present annotation methods can be applicable for the neuropeptide annotation of other insects. Furthermore, the methods will help to generate useful infrastructures for studies relevant to neuropeptides.
Ultra-Low-Power Architecture for the Detection and Notification of Wildfires Using the Internet of Things
Authors: Khan Tareq
Published: January 2023

Wildfires kill and injure people, destroy residences, pollute the air, and cause economic loss. In this paper, a low-power Internet of Things (IoT)-based sensor network is developed, which automatically detects fires in forests and sends the location to a central monitoring station with smartphone notifications in a real-time setting. This action helps in the early detection of a fire and firefighters can be notified immediately—thus the spread of the fire and the harm caused by it can be reduced. The proposed system detects fires from the presence of smoke and a sudden increase in temperature. The system also logs the temperature, humidity, carbon dioxide, rain, light, and wind speed in different areas of the forest. The sensor nodes transmit the data to a hub using a long-range wireless transmitter and the hub then sends the data to the central monitoring station using the cellular Internet. The sensor nodes and hub are designed with ultra-low-power hardware and software architecture, consuming current of only 0.37 and 1.4 mA, respectively, so that they can be powered by solar panels throughout the year. The central server and smartphone app contain maps, and the wildfire locations are marked in the case of a fire. In the present study, a prototype of the proposed system is successfully developed and tested.
Diabetic Retinopathy: Soluble and Imaging Ocular Biomarkers
Authors: Ferrara Mariantonia , Loda Alessandra , Coco Giulia , Grassi Piergiacomo , Cestaro Silvia , Rezzola Sara , Romano Vito , Semeraro Francesco
Published: January 2023

Diabetic retinopathy (DR), the most common microvascular complication of diabetes mellitus, represents the leading cause of acquired blindness in the working-age population. Due to the potential absence of symptoms in the early stages of the disease, the identification of clinical biomarkers can have a crucial role in the early diagnosis of DR as well as for the detection of prognostic factors. In particular, imaging techniques are fundamental tools for screening, diagnosis, classification, monitoring, treatment planning and prognostic assessment in DR. In this context, the identification of ocular and systemic biomarkers is crucial to facilitate the risk stratification of diabetic patients; moreover, reliable biomarkers could provide prognostic information on disease progression as well as assist in predicting a patient’s response to therapy. In this context, this review aimed to provide an updated and comprehensive overview of the soluble and anatomical biomarkers associated with DR.
A Novel Heterozygous Desmoplakin Variant Causes Cardiocutaneous Syndrome with Arrhythmogenic Cardiomyopathy and Palmoplantar Keratosis
Authors: Çimen Tolga , Medeiros-Domingo Argelia , Kolios Antonios , Akdiş Deniz , Anwer Shehab , Tanner Felix C. , Brunckhorst Corinna , Duru Firat , Saguner Ardan M.
Published: January 2023

Cardiocutaneous syndrome (CCS) is often caused by genetic variants in desmoplakin (DSP) in the presence of thick calluses on the hands and soles of the feet (palmoplantar keratoderma) in combination with arrhythmogenic cardiomyopathy. In this case report, we describe a 58-year-old man presenting with a history of cardiomyopathy with recurrent sustained ventricular tachycardia and palmoplantar keratosis. The cardiological evaluation showed biventricular cardiomyopathy, and repeated genetic testing identified a novel DSP variant. Repeated genetic testingis clinically meaningful in patients with a high probability of a specific inherited cardiac disease, such as CCS, particularly if molecular screening has been performed in the pre-NGS era with an incomplete NGS panel or outdated technology as presented in this case report.
Vitamin D, Menopausal Health and COVID-19: Critical Appraisal of Current Data
Authors: Anagnostis Panagiotis , Livadas Sarantis , Goulis Dimitrios G. , Rees Margaret , Lambrinoudaki Irene
Published: January 2023

Inconsistency exists across studies conducted in postmenopausal women regarding the effect of vitamin D deficiency (VDD) and supplementation on several aspects of menopausal health, such as fractures, vasomotor symptomatology, cardiovascular disease (CVD), cancer and infections, including coronavirus disease 2019 (COVID-19). The aim of this review is to critically summarize the evidence provided by observational studies and randomized controlled trials (RCTs) of vitamin D supplementation in postmenopausal women with VDD. Observational studies have found that VDD is associated with an increased risk of falls and fractures after the menopause. VDD also has a negative effect on menopausal symptomatology. VDD, especially its severe form, is associated with an increased risk of CVD risk factors and CVD events. VDD is associated with increased risk and mortality from several cancer types and risk of infections. The evidence from RCTs regarding the effect of vitamin D supplementation on falls, fractures, menopausal symptoms, cardiovascular disease, cancer and infections is not robust. Thus, skeletal health may benefit only when vitamin D is co-administered with calcium, especially in those ≥70 years old and with severe VDD. There is no evidence of a favorable effect on menopausal symptoms or risk of CVD or cancer, except for a modest reduction in cancer-related mortality. Inconsistency still exists regarding its effect on infection risk, disease severity and mortality due to COVID-19.
Microspheres as a Carrier System for Therapeutic Embolization Procedures: Achievements and Advances
Authors: Welling Mick. M. , Duszenko Nikolas , van Meerbeek Maarten P. , Molenaar Tom J. M. , Buckle Tessa , van Leeuwen Fijs W. B. , Rietbergen Daphne D. D.
Published: January 2023

The targeted delivery of anti-cancer drugs and isotopes is one of the most pursued goals in anti-cancer therapy. One of the prime examples of such an application is the intra-arterial injection of microspheres containing cytostatic drugs or radioisotopes during hepatic embolization procedures. Therapy based on the application of microspheres revolves around vascular occlusion, complemented with local therapy in the form of trans-arterial chemoembolization (TACE) or radioembolization (TARE). The broadest implementation of these embolization strategies currently lies within the treatment of untreatable hepatocellular cancer (HCC) and metastatic colorectal cancer. This review aims to describe the state-of-the-art TACE and TARE technologies investigated in the clinical setting for HCC and addresses current trials and new developments. In addition, chemical properties and advancements in microsphere carrier systems are evaluated, and possible improvements in embolization therapy based on the modification of and functionalization with therapeutical loads are explored.
Assessment Tools for the Admission of Older Adults to Inpatient Rehabilitation: A Scoping Review
Authors: Muscat Francesca , Camilleri Liberato , Attard Conrad , Lungaro Mifsud Stephen
Published: January 2023

(1) Objective: To identify the assessment tools and outcome measures used to assess older adults for inpatient rehabilitation. (2) Design: Scoping review. (3) Data sources: ProQuest, PEDro, PubMed, CINAHL Plus with full text (EBSCO), Cochrane Library and reference lists from included studies. (4) Review method: The inclusion of studies covering patients aged >60, focusing on rehabilitation assessments delivered in hospitals in community settings. Studies reporting on rehabilitation specifically designed for older adults—testing for at least one domain that affects rehabilitation or assessments for admission to inpatient rehabilitation—were also included. Results were described both quantitatively and narratively. (5) Results: 1404 articles were identified through selected databases and registers, and these articles underwent a filtering process intended to identify and remove any duplicates. This process reduced the number to 1186 articles. These, in turn, were screened for inclusion criteria, as a result of which 37 articles were included in the final review. The majority of assessments for geriatric rehabilitation were carried out by a multidisciplinary team. Multiple studies considered more than one domain during assessment, with a high percentage evaluating a specific outcome measure used in geriatric rehabilitation. The most common domains assessed were function, cognition and medical status—with communication, vision and pain being the least common. A total of 172 outcome measures were identified in this review, with MMSE, BI, FIM and CCI being the most frequent. (6) Conclusions: This review highlights the lack of standardised approaches in existing assessment processes. Generally, older-adult-rehabilitation assessments struggle to capture rehabilitation potential in a holistic manner. Hence, a predictive model of rehabilitation for assessing patients at the initial stages would be useful in planning a patient-specific programme aimed at maximising functional independence and, thus, quality of life.
Association of Anemia with Clinical Symptoms Commonly Attributed to Anemia—Analysis of Two Population-Based Cohorts
Authors: Weckmann Gesine , Kiel Simone , Chenot Jean-François , Angelow Aniela
Published: January 2023

Background: Fatigue, dyspnea, and lack of energy and concentration are commonly interpreted as indicative of symptomatic anemia and may thus play a role in diagnostic and therapeutic decisions. Objective: To investigate the association between symptoms commonly attributed to anemia and the actual presence of anemia. Methods: Data from two independent cohorts of the Study of Health in Pomerania (SHIP) were analyzed. Interview data, laboratory data, and physical examination were individually linked with claims data from the Association of Statutory Health Insurance Physicians. A complete case analysis using logistic regression models was performed to evaluate the association of anemia with symptoms commonly attributed to anemia. The models were adjusted for confounders such as depression, medication, insomnia, and other medical conditions. Results: A total of 5979 participants (53% female, median age 55) were included in the analysis. Of those, 30% reported fatigue, 16% reported lack of energy, 16% reported lack of concentration, and 29% reported dyspnea and/or weakness. Anemia was prevalent in about 6% (379). The symptoms were more prevalent in participants with anemia. However, participants with anemia were older and had a poorer health status. There was no association in multivariate logistic regression models between the symptoms fatigue, lack of concentration, dyspnea, and/or weakness and anemia. Anemia was associated (OR: 1.45; 95% CI: 1.13–1.86) with lack of energy in the multivariate analysis. Other factors such as depression, insomnia, and medication were more strongly associated with the symptoms. Conclusion: The clinical symptoms commonly attributed to anemia are unspecific and highly prevalent both in non-anemic and anemic persons. Even in the presence of anemia, other diagnoses should be considered as causes such as depression, heart failure, asthma, and COPD, which are more closely associated with the symptoms. Further diagnostic research is warranted to explore the association of symptoms in different subgroups and settings in order to help clinical decision making.
Gas Chromatography–Mass Spectrometry (GC–MS) Metabolites Analysis in Endometriosis Patients: A Prospective Observational Translational Study
Authors: Angioni Stefano , Congiu Francesca , Vitale Salvatore Giovanni , D’Alterio Maurizio Nicola , Noto Antonio , Monni Giovanni , Santoru Maria Laura , Fanos Vassilios , Murgia Federica , Atzori Luigi
Published: January 2023

Background: Endometriosis affects women of reproductive age, and its pathogenesis is still unclear. Typically, it overlaps other similar medical and surgical conditions, determining a delay in early diagnosis. Metabolomics allows studying metabolic changes in different physiological or pathological states to discover new potential biomarkers. We used the gas chromatography–mass spectrometer (GC–MS) to explore metabolic alterations in endometriosis to better understand its pathophysiology and find new biomarkers. Methods: Twenty-two serum samples of patients with symptomatic endometriosis and ten without it were collected and subjected to GC–MS analysis. Multivariate and univariate statistical analyses were performed, followed by pathway analysis. Results: Partial least squares discriminant analysis was performed to determine the differences between the two groups (p = 0.003). Threonic acid, 3-hydroxybutyric acid, and proline increased significantly in endometriosis patients, while alanine and valine decreased. ROC curves were built to test the diagnostic power of metabolites. The pathway analysis identified the synthesis and degradation of ketone bodies and the biosynthesis of phenylalanine, tyrosine, and tryptophan as the most altered pathways. Conclusions: The metabolomic approach identifies metabolic alterations in women with endometriosis. These findings may improve our understanding of the pathophysiological mechanisms of disease and the discovery of new biomarkers.
Soft-Tissue Augmentation around Dental Implants with a Connective Tissue Graft (CTG) and Xenogeneic Collagen Matrix (CMX)—5-Year Follow-Up
Authors: Hadzik Jakub , Błaszczyszyn Artur , Gedrange Tomasz , Dominiak Marzena
Published: January 2023

Proper horizontal and vertical thickness of the gingival connective tissue has been proven to be one of the success criteria in dental implant and reconstructive surgery. When thin tissue is found, gingiva augmentation methods can be used to increase the quality and volume of the tissue. Many methods have been described, among them pedicle soft-tissue flaps or autogenic tissue grafts. As an alternative to patients’ own tissue, xenogenic materials can be used for grafting. The fundamental issue is to choose a material that will ensure the maximum therapeutic effect, while also minimizing the negative influence on the patient’s health. The aim of this study was to compare gingival augmentation procedures using a palatal connective tissue graft (CTG) and an xenogenic soft-tissue substitute, Geistlich Mucograft (xenogeneic collagen matrix; CMX), and assess whether the timing of the graft surgery influences the clinical outcomes. The original study was a randomized control trial with a total of 75 implants placed. The patients received the soft-tissue thickening 3 months before the implant placement or 3 months after the implant placement (depending on the group). A connective tissue graft (CTG) or Geistlich Mucograft were used (depending on the group). For both the CTG and Geistlich Mucograft, better clinical outcomes were observed for maintaining the alveolar bone level and the thickness of the attached gingiva compared to the control group with no gingival augmentation. The Geistlich Mucograft showed good clinical performance in comparison to the control. Soft-tissue augmentation with the CTG before the implant placement was found to be most efficient method in terms of a stable increase of the tissue thickness since, throughout the entire observation period, the greatest increase of 1.035 mm (SD = 0.73 mm) in thickness was observed. Statistically important differences in the tissue thickness baseline compared after 5 years were observed for groups G1 vs. G2b (no augmentation vs. CTG before), G1 vs. G3b (no augmentation vs. CTG after) and for groups G2b vs. G3a (CTG before vs. CMX after).
Assessment of the Potential of Waste Copper Chromium and Arsenic (CCA)-Treated Timber Fibre Reinforced Polypropylene Composites for Construction
Authors: Nelson Jacob , Pickering Kim L. , Beg Mohammad D. H.
Published: January 2023

This paper investigates the potential of recycling waste copper chromium and arsenic (CCA)-treated timber for use as a reinforcement material in wood–plastic composites (WPCs) produced for use in construction, including an assessment of mechanical properties and the leaching of heavy metals. Wood flour was obtained through mechanical grinding, and fibres were obtained through alkaline digestion followed by bleaching. Composites produced with 40 wt.% bleached fibres showed increased tensile strength from 18.5 MPa for the polypropylene used as the matrix to 27.6 MPa. Likewise, the Young’s modulus was increased from 0.84 to 2.33 GPa. The treatment of fibres was found to reduce arsenic concentration by up to 99.9%. Furthermore, the arsenic in the leachate from composites was found to decrease from 41.29 to 0.07 ppb when comparing CCA-treated wood flour composites to bleached fibre composites. The composites’ material properties indicate that the use of end-of-life CCA-treated timber could be used to produce a composite material that could be used in New Zealand’s building sector to meet the requirements of semi-structural applications.
A Real-Time Polyp-Detection System with Clinical Application in Colonoscopy Using Deep Convolutional Neural Networks
Authors: Krenzer Adrian , Banck Michael , Makowski Kevin , Hekalo Amar , Fitting Daniel , Troya Joel , Sudarevic Boban , Zoller Wolfgang G. , Hann Alexander , Puppe Frank
Published: January 2023

Colorectal cancer (CRC) is a leading cause of cancer-related deaths worldwide. The best method to prevent CRC is with a colonoscopy. During this procedure, the gastroenterologist searches for polyps. However, there is a potential risk of polyps being missed by the gastroenterologist. Automated detection of polyps helps to assist the gastroenterologist during a colonoscopy. There are already publications examining the problem of polyp detection in the literature. Nevertheless, most of these systems are only used in the research context and are not implemented for clinical application. Therefore, we introduce the first fully open-source automated polyp-detection system scoring best on current benchmark data and implementing it ready for clinical application. To create the polyp-detection system (ENDOMIND-Advanced), we combined our own collected data from different hospitals and practices in Germany with open-source datasets to create a dataset with over 500,000 annotated images. ENDOMIND-Advanced leverages a post-processing technique based on video detection to work in real-time with a stream of images. It is integrated into a prototype ready for application in clinical interventions. We achieve better performance compared to the best system in the literature and score a F1-score of 90.24% on the open-source CVC-VideoClinicDB benchmark.
Simulated Study of the Machinability of the Nomex Honeycomb Structure
Authors: Zarrouk Tarik , Nouari Mohammed , Makich Hamid
Published: January 2023

The Nomex honeycomb core has been widely used in many industrial fields, especially the aircraft and aerospace industries, due to its high strength and stiffness to heaviness ratio. Machining of the Nomex honeycomb structure is usually associated with tearing of the walls, deformations of the cells and the appearance of burrs. Therefore, milling of the Nomex honeycomb structure represents an industrial hurdle challenge for scientists and researchers about the quality of the machined surface and the integrity of the cutting tool. In response to this problem, we have developed a three-dimensional numerical model of finite elements based on the real conditions of experimental work by means of the analysis software Abaqus. Based on the developed numerical model, an experimental validation was performed by comparing the quality of the surface and the adhesive wear of the cutting tool determined from the numerical simulation and that established by the experiment. In addition, the effect of geometric parameters in terms of wedge angle and cutting tool diameter on material accumulation, chip size, generated surface and cutting forces was analyzed. The results of the quantitative analysis prove that the choice of cutting conditions and cutting tool geometry in terms of favorable rake angle and tool diameter improves the surface quality of the generated part and optimizes the integrity of the cutting tool.
Raising the Standard of Maritime Voyage Data Recorder Security
Authors: Hopcraft Rory , Harish Avanthika Vineetha , Tam Kimberly , Jones Kevin
Published: January 2023

Voyage Data Recorders (VDRs), often referred to as the ‘black boxes’ of the shipping industry, collect and store vital data from key sensors and locations around the ship. This data plays a pivotal role in incident investigation, as was seen in the grounding of the Costa Concordia in 2012, and the sinking of the El Faro in 2015. With such an important role to play, the International Maritime Organization (IMO) has mandated that all SOLAS registered ships carry a VDR, which can demonstrate compliance with internationally agreed standards. Without a VDR compliant with these standards a ship cannot sail. However, the rise in the number, and sophistication, of digital devices are making the sector increasingly vulnerable to cyber-attacks. This paper will demonstrate a number of high-risk VDR cyber security vulnerabilities and review the current international technical standards covering all VDR devices being manufactured and used today, drawing attention to the minimum security requirements. The paper will go on to discuss how these standards fail to promote the necessary levels of cyber security needed to protect VDRs from today’s cyber risks, amidst increased demands for digital connectivity for remote and autonomous operations. The paper will conclude by proposing several amendments (technical and non-technical) to the current standards which, if adopted, will help increase the minimum level of security of VDRs. Industry opinions were gathered on this topic, and their beliefs have been included across this paper.
Comparison of Surgical Outcomes between Single-Port Laparoscopic Surgery and Da Vinci Single-Port Robotic Surgery
Authors: Kim Jeong-Min , Lee Seon-Mi , Seol Aeran , Song Jae-Yun , Ryu Ki-Jin , Lee Sanghoon , Park Hyun-Tae , Cho Hyun-Woong , Min Kyung-Jin , Hong Jin-Hwa , Lee Jae-Kwan , Lee Nak-Woo
Published: January 2023

Background: The aim of this study is to compare the surgical outcomes of single-port laparoscopic surgery (SPLS) and single-port robotic surgery (SPRS). Methods: We retrospectively analyzed patients who underwent a hysterectomy, ovarian cystectomy, or myomectomy with SPLS or SPRS from January 2020 to July 2022. Statistical analyses were performed using the SPSS chi-square test and student’s t-test. Results: A total of 566 surgeries including single-port laparoscopic hysterectomy (SPLH; n = 148), single-port robotic hysterectomy (SPRH; n = 35), single-port laparoscopic ovarian cystectomy (SPLC; n = 207), single-port robotic ovarian cystectomy (SPRC; n = 108), single-port laparoscopic myomectomy (SPLM; n = 12), and single-port robotic myomectomy (SPRM; n = 56). The SPRH, SPRC, and SPRM groups had a shorter operation time than the SPLS group, although the results were not statistically significant (SPRH vs. SPLH, p = 0.134; SPRC vs. SPLC, p = 0.098; SPRM vs. SPLM, p = 0.202). Incisional hernia occurred as a postoperative complication in two patients only in the SPLH group. Postoperative Hb changes were lower in the SPRC and SPRM groups than in the SPLC and SPLM groups (SPRC vs. SPLC, p = 0.023; SPRM vs. SPLM, p = 0.010). Conclusions: Our study demonstrated that the SPRS had comparable surgical outcomes when compared to the SPLS. Therefore, the SPRS should be considered a feasible and safe option for gynecologic patients.
Paths of Evolution of Progressive Anaplastic Meningiomas: A Clinical and Molecular Pathology Study
Authors: Di Bonaventura Rina , Lauretti Liverana , Martini Maurizio , Cenci Tonia , Di Monaco Giuliano , Palombi Davide , Ceccarelli Giovanni Maria , Chiesa Silvia , Gessi Marco , Granitto Alessia , Albanese Alessio , Larocca Luigi Maria , D’Alessandris Quintino Giorgio , Pallini Roberto , Olivi Alessandro
Published: January 2023

Grade 3 meningiomas are rare malignant tumors that can originate de novo or from the progression of lower grade meningiomas. The molecular bases of anaplasia and progression are poorly known. We aimed to report an institutional series of grade 3 anaplastic meningiomas and to investigate the evolution of molecular profile in progressive cases. Clinical data and pathologic samples were retrospectively collected. VEGF, EGFR, EGFRvIII, PD-L1; and Sox2 expression; MGMT methylation status; and TERT promoter mutation were assessed in paired meningioma samples collected from the same patient before and after progression using immunohistochemistry and PCR. Young age, de novo cases, origin from grade 2 in progressive cases, good clinical status, and unilateral side, were associated with more favorable outcomes. In ten progressive meningiomas, by comparing molecular profile before and after progression, we identified two subgroups of patients, one defined by Sox2 increase, suggesting a stem-like, mesenchymal phenotype, and another defined by EGFRvIII gain, suggesting a committed progenitor, epithelial phenotype. Interestingly, cases with Sox2 increase had a significantly shortened survival compared to those with EGFRvIII gain. PD-L1 increase at progression was also associated with worse prognosis, portending immune escape. We thus identified the key drivers of meningioma progression, which can be exploited for personalized treatments.
A Scenario Simulation Study on the Impact of Urban Expansion on Terrestrial Carbon Storage in the Yangtze River Delta, China
Authors: Ma Zhiyuan , Duan Xuejun , Wang Lei , Wang Yazhu , Kang Jiayu , Yun Ruxian
Published: January 2023

Assessing the impacts and drivers of urban expansion on terrestrial carbon storage (TCS) is important for urban ecology and sustainability; however, a unified accounting standard for carbon intensity and research on the drivers and economic value of TCS changes are lacking. Here, urban expansion and TCS in the Yangtze River Delta were simulated based on Patch-generating Land Use Simulation and Integrated Valuation of Ecosystem Services and Trade-offs models; scenario simulation; Literature, Correction, Ratio, Verification carbon intensity measurement; and land use transfer matrix methods. The results showed that (1) from 2000 to 2020, urbanization and TCS loss accelerated, with 61.127% of TCS loss occurring in soil, and land conversion was prominent in riverine and coastal cities, mainly driven by the urban land occupation of cropland around suitable slopes, transportation arteries, and rivers. (2) From 2020 to 2030, urban land expansion and TCS loss varied under different scenarios; economic losses from the loss of the carbon sink value under cropland protection and ecological protection were USD 102.368 and 287.266 million lower, respectively, than under the baseline scenario. Even if urban expansion slows, the loss of TCS under global warming cannot be ignored. Considering the indirect impacts of urbanization, the failure to establish a regional development master plan based on ecosystem services may affect China’s carbon targets.
Vine-Twining Inclusion Behavior of Amylose towards Hydrophobic Polyester, Poly(β-propiolactone), in Glucan Phosphorylase-Catalyzed Enzymatic Polymerization
Authors: Iwamoto Masa-aki , Kadokawa Jun-ichi
Published: January 2023

This study investigates inclusion behavior of amylose towards, poly(β-propiolactone) (PPL), that is a hydrophobic polyester, via the vine-twining process in glucan phosphorylase (GP, isolated from thermophilic bacteria, Aquifex aeolicus VF5)-catalyzed enzymatic polymerization. As a result of poor dispersibility of PPL in sodium acetate buffer, the enzymatically produced amylose by GP catalysis incompletely included PPL in the buffer media under the general vine-twining polymerization conditions. Alternatively, we employed an ethyl acetate–sodium acetate buffer emulsion system with dispersing PPL as the media for vine-twining polymerization. Accordingly, the GP (from thermophilic bacteria)-catalyzed enzymatic polymerization of an α-d-glucose 1-phosphate monomer from a maltoheptaose primer was performed at 50 °C for 48 h in the prepared emulsion to efficiently form the inclusion complex. The powder X-ray diffraction profile of the precipitated product suggested that the amylose-PPL inclusion complex was mostly produced in the above system. The 1H NMR spectrum of the product also supported the inclusion complex structure, where a calculation based on an integrated ratio of signals indicated an almost perfect inclusion of PPL in the amylosic cavity. The prevention of crystallization of PPL in the product was suggested by IR analysis, because it was surrounded by the amylosic chains due to the inclusion complex structure.
Adherence to CPAP Treatment: Can Mindfulness Play a Role?
Authors: Pataka Athanasia , Kotoulas Seraphim Chrysovalantis , Gavrilis Panagiotis Raphael , Karkala Alice , Tzinas Asterios , Stefanidou Aimiliza
Published: January 2023

Obstructive sleep apnea (OSA) is considered a chronic disease that requires long-term multidisciplinary management for effective treatment. Continuous Positive Airway Pressure (CPAP) is still considered the gold standard of therapy. However, CPAP effectiveness is limited due to poor patients’ adherence, as almost 50% of patients discontinue treatment after a year. Several interventions have been used in order to increase CPAP adherence. Mindfulness-based therapies have been applied in other sleep disorders such as insomnia but little evidence exists for their application on OSA patients. This review aims to focus on the current data on whether mindfulness interventions may be used in order to increase CPAP adherence and improve the sleep quality of OSA patients. Even though controlled trials of mindfulness and CPAP compliance remain to be performed, this review supports the hypothesis that mindfulness may be used as an adjunct method in order to increase CPAP adherence in OSA patients.
Chronic Neuroinflammation and Cognitive Decline in Patients with Cardiac Disease: Evidence, Relevance, and Therapeutic Implications
Authors: Traub Jan , Frey Anna , Störk Stefan
Published: January 2023

Acute and chronic cardiac disorders predispose to alterations in cognitive performance, ranging from mild cognitive impairment to overt dementia. Although this association is well-established, the factors inducing and accelerating cognitive decline beyond ageing and the intricate causal pathways and multilateral interdependencies involved remain poorly understood. Dysregulated and persistent inflammatory processes have been implicated as potentially causal mediators of the adverse consequences on brain function in patients with cardiac disease. Recent advances in positron emission tomography disclosed an enhanced level of neuroinflammation of cortical and subcortical brain regions as an important correlate of altered cognition in these patients. In preclinical and clinical investigations, the thereby involved domains and cell types of the brain are gradually better characterized. Microglia, resident myeloid cells of the central nervous system, appear to be of particular importance, as they are extremely sensitive to even subtle pathological alterations affecting their complex interplay with neighboring astrocytes, oligodendrocytes, infiltrating myeloid cells, and lymphocytes. Here, we review the current evidence linking cognitive impairment and chronic neuroinflammation in patients with various selected cardiac disorders including the aspect of chronic neuroinflammation as a potentially druggable target.
Impact of Obesity on Clinical Outcomes of Patients with Intra-Abdominal Hypertension and Abdominal Compartment Syndrome
Authors: Mohan Swetha , Lim Zavier Yongxuan , Chan Kai Siang , Shelat Vishal G.
Published: January 2023

Intra-abdominal hypertension (IAH) and abdominal compartment syndrome (ACS) are associated with high morbidity and mortality. Obesity may result in increased intra-abdominal pressure (IAP) and affect clinical outcomes of patients with IAH and/or ACS. This study aims to establish the impact of obesity on the clinical outcomes of IAH and ACS patients. A systematic search of Medline, Embase, and Scopus was performed in August 2022. Nine studies comprising 9938 patients were included. There were 65.1% males (n = 6250/9596). Patient demographics, comorbidities, and morbidities were analyzed in correlation with obesity and IAP. Obese patients had a higher risk of IAH (OR 8.5, p < 0.001). Obesity was associated with the need for renal replacement therapy, intensive care unit-acquired infections, systemic inflammatory response syndrome, acute respiratory distress syndrome, length of hospital stay, and mortality. This review highlights the lacunae in the existing literature to underpin the direct impact of obesity, independent of obesity-associated comorbidities, on the clinical outcomes of IAH and ACS.
Experimental and Numerical Study of the Mixed Lubrication Considering Boundary Film Strength
Authors: Zhang Shengwei , Yan Zhijun , Liu Ze , Jiang Yuanyuan , Sun Haocheng , Wu Shibo
Published: January 2023

For the influence of boundary film on the lubrication state of sliding friction pairs, a boundary film strength model was proposed that can comprehensively reflect the influences of film thickness, pressure, shear stress and temperature. The model parameters were obtained through fitting the test results. Then, a mixed lubrication model considering boundary film strength was established by coupling the boundary film strength model with the hydrodynamic lubrication model and the asperity contact model. The calculation program was developed using the Fortran language, which can effectively capture the tribological characteristics and action ratios of the fluid, boundary film and dry friction components. Simultaneously, the mixed lubrication model was applied to the journal bearing. A parametric analysis was performed to investigate the influences of different working conditions on lubrication performance. Under current operating conditions, the results show that: when the speed is above 200 r/min or the viscosity is higher than 0.09 Pa·s, the boundary film breakdown rate is almost 0 and the friction coefficient is lower than 0.02; when the roughness is reduced from 1.8 μm to 0.8 μm, the ultimate load of the journal bearing rises from 27 MPa to 36 MPa, an increase of about 33%; when the load exceeds 36 MPa or the temperature is higher than 100 °C, more than 25% of the boundary film breaks and the dry friction component accounts for more than 60% of the total friction, which leads to a sudden increase in the friction coefficient. Hence, the study of mixed lubrication considering boundary film strength provides theoretical guidance for accurately reflecting the actual lubrication state and improving the mechanical energy efficiency of friction pairs.
Experimental Study of Thermally Damaged Concrete under a Hygrothermal Environment by Using a Combined Infrared Thermal Imaging and Ultrasonic Pulse Velocity Method
Authors: Wang Yi , Cui Jiajie , Deng Jun , Zhou Hao
Published: January 2023

This paper proposes a combined inspection method for thermally damaged concrete under a hygrothermal environment. Experiments were conducted to verify the feasibility of the proposed method. Concrete samples with different water–cement ratios (W/C = 0.3, 0.5, 0.7) and moisture contents (dried, 50% saturated, fully saturated) were exposed to elevated temperatures of 200 °C, 400 °C, 600 °C, and 800 °C for 4 h. After cooling to room temperature, infrared thermal imaging (IRT), ultrasonic pulse velocity (UPV) measurements, and mechanical tests were carried out for the damaged concrete samples. The mechanical behavior of thermally damaged concrete with different degrees of water saturation was examined based on mechanical testing. The results show that water can affect the compressive strength and UPV of concrete under certain circumstances, and the residual strength and the heating temperature of the thermally damaged concrete can be evaluated by IRT and UPV measurements. When 50% saturated concrete specimens with a W/C ratio of 0.3, 0.5, and 0.7 are exposed to 200 °C, 12.6%, 27.4%, and 34.6% increases in normalized compressive strength were observed before dropping to approximately 40% at 800 °C. With various moisture contents, the normalized compressive strength variation can be up to 40% at 400 °C in cases with W/C = 0.5 and 0.7. As for UPV, it generally decreases with the increase in moisture content when the peak temperature is 800 °C. On the contrary, whether concrete is saturated or not, there is little difference in temperature change in IRT detection. To obtain a more precise evaluation of concrete structures, IRT can be used to scan a large area to determine the damaged concrete area and areas suspected to be damaged, while UPV could be used to detect concrete members in suspected areas after the completion of IRT scanning.
Explainable Data-Driven Method Combined with Bayesian Filtering for Remaining Useful Lifetime Prediction of Aircraft Engines Using NASA CMAPSS Datasets
Authors: Maulana Faisal , Starr Andrew , Ompusunggu Agusmian Partogi
Published: January 2023

An aircraft engine is expected to have a high-reliability system as a safety-critical asset. A scheduled maintenance strategy based on statistical calculation has been employed as the current practice to achieve the reliability requirement. Any improvement to this maintenance interval is made after significant reliability issues arise (such as flight delays and high component removals). Several publications and research studies have been conducted related to this issue, one of them involves performing simulations and providing aircraft operation datasets. The recently published NASA CMAPPS datasets have been utilised in this paper since they simulate flight data recording from various measurements. A prognostics model can be developed by analysing these datasets and predicting the engine’s reliability before failure. However, the state-of-the-art prognostics techniques published in the literature using these NASA CMAPPS datasets are mainly purely data-driven. These techniques mainly deal with a “black box” process which does not include uncertainty quantification (UQ). These two factors are barriers to prognostics applications, particularly in the aviation industry. To tackle these issues, this paper aims at developing explainable and transparent algorithms and a software tool to compute the engine health, estimate engine end of life (EoL), and eventually predict its remaining useful life (RUL). The proposed algorithms use hybrid metrics for feature selection, employ logistic regression for health index estimation, and unscented Kalman filter (UKF) to update the prognostics model for predicting the RUL in a recursive fashion. Among the available datasets, dataset 02 is chosen because it has been widely used and is an ideal candidate for result comparison and dataset 03 is employed as a new state-of-the-art. As a result, the proposed algorithms yield 34.5–55.6% better performance in terms of the root mean squared error (RMSE) compared with the previous work. More importantly, the proposed method is transparent and it quantifies the uncertainty during the prediction process.
An Investigation into the Multi-Pass Radial-Mode Micro Abrasive Air Jet Turning of Fused-Silica Rods
Authors: Yang Ruibo , Li Quanlai , Zhang Weipeng , Deng Yafeng , Li Jialin
Published: January 2023

With the increased requirement of miniaturization structures on hard and brittle substrates, micro abrasive air jet turning technology has become a promising machining technology for manufacturing miniaturization structures. This paper presents an investigation on multi-pass radial-mode micro abrasive air jet turning of fused silica. The effect of machining parameters on the depth of cut and materials removal rate was analyzed. The waviness and striation formation mechanisms were studied. It was found that increasing the number of passes significantly increases the depth of cut and materials removal rate. When the surface speed is reduced, the depth of cut and materials removal rate significantly increases. The waviness may be attributed to the intersection and overlap of the adjoint jet footprint. The crossed striations have been found, which may lead to the elimination of the striation. A predictive model of the material removal rate was developed using the dimensional analysis method. The model was assessed and shown to be able to give adequate predictions.
Temporomandibular Disorders: Current and Future Concepts in Diagnosis and Management
Authors: Ferrillo Martina
Published: January 2023

Temporomandibular disorders (TMD) are musculoskeletal and/or neuromuscular conditions relating to muscles, joints, and the associated structures of the stomatognathic system [...]
Mechanism of Action and Structure–Activity Relationships of Tetracyclic Small Molecules Acting as Universal Positive Allosteric Modulators of the Cholecystokinin Receptor
Authors: Dengler Daniela G. , Harikumar Kaleeckal G. , Yen Alice , Sergienko Eduard A. , Miller Laurence J.
Published: January 2023

As part of an ongoing effort to develop a drug targeting the type 1 cholecystokinin receptor (CCK1R) to help prevent and/or treat obesity, we recently performed a high throughput screening effort of small molecules seeking candidates that enhanced the action of the natural agonist, CCK, thus acting as positive allosteric modulators without exhibiting intrinsic agonist action. Such probes would be expected to act in a temporally finite way to enhance CCK action to induce satiety during and after a meal and potentially even modulate activity at the CCK1R in a high cholesterol environment present in some obese patients. The current work focuses on the best scaffold, representing tetracyclic molecules identified through high throughput screening we previously reported. Extensive characterization of the two top “hits” from the previous effort demonstrated them to fulfill the desired pharmacologic profile. We undertook analog-by-catalog expansion of this scaffold using 65 commercially available analogs. In this effort, we were able to eliminate an off-target effect observed for this scaffold while retaining its activity as a positive allosteric modulator of CCK1R in both normal and high cholesterol membrane environments. These insights should be useful in the rational medicinal chemical enhancement of this scaffold and in the future development of candidates to advance to pre-clinical proof-of-concept and to clinical trials.
Odor Discrimination by Lipid Membranes
Authors: Lowry Troy W. , Kusi-Appiah Aubrey E. , Fadool Debra Ann , Lenhert Steven
Published: January 2023

Odor detection and discrimination in mammals is known to be initiated by membrane-bound G-protein-coupled receptors (GPCRs). The role that the lipid membrane may play in odor discrimination, however, is less well understood. Here, we used model membrane systems to test the hypothesis that phospholipid bilayer membranes may be capable of odor discrimination. The effect of S-carvone, R-carvone, and racemic lilial on the model membrane systems was investigated. The odorants were found to affect the fluidity of supported lipid bilayers as measured by fluorescence recovery after photobleaching (FRAP). The effect of odorants on surface-supported lipid multilayer microarrays of different dimensions was also investigated. The lipid multilayer micro- and nanostructure was highly sensitive to exposure to these odorants. Fluorescently-labeled lipid multilayer droplets of 5-micron diameter were more responsive to these odorants than ethanol controls. Arrays of lipid multilayer diffraction gratings distinguished S-carvone from R-carvone in an artificial nose assay. Our results suggest that lipid bilayer membranes may play a role in odorant discrimination and molecular recognition in general.