bluuebunny/crossref_metadata_2025 huggingface dataset:
Spawning sources of a coastal fishery species inferred from otolith chemistry and microstructure: implications for management
Authors: Jenkins GP , Hamer PA , Kent JA , Kemp J , Sherman CDH , Fowler AJ
Published: November 2021

Spawning sources of King George whiting Sillaginodes punctatus populations in the states of South Australia and Victoria (south-eastern Australia) were analysed using otolith chemistry and microstructure from post-larvae sampled from 3 nursery areas in each state in the spring of 2011 and 2012. Univariate and multivariate analysis of the chemistry of the core region of otoliths showed differences between states, particularly for the 2011 cohort, primarily related to higher Mg in South Australian samples, while differences in Sr and Zn also made a contribution. Even though spawning times overlapped, early larval growth rates were higher for post-larvae from South Australia than Victoria. Differences in microchemistry were most evident for elements influenced by physiological processes and were potentially influenced by the different larval growth rates. Overall, otolith chemical and microstructure analyses for post-larvae in Victoria and South Australia indicated that spawning sources for the 2 states were different, qualified by results from otolith microchemistry that were less clear for the 2012 cohort. Even though genetic analyses do not indicate genetic differentiation across the 2 states, and therefore would support cross-jurisdictional management, the results of this study give qualified support to the current arrangement wherein the S. punctatus fishery is managed separately by the individual jurisdictions, subject to further information on stock structure coming to light in the future.
Personalising Exercise and Nutrition Behaviours in Diabetes Lifestyle Prevention
Authors: Alkhatib Ahmad
Published: November 2021

The alarming increase in global diabetes, especially Type 2 diabetes mellitus (T2DM), is affecting diverse populations and leading to consequent burdens of morbidities, mortalities, and healthcare costs. Physical activity and nutritional approaches form the cornerstones of lifestyle T2DM prevention. Advances in understanding an individual's behaviour and biological responses to different exercise conditions are concurrent with new personalised exercise and nutritional and behavioural tools effective for preventing T2DM and associated chronic diseases. Targeted exercise and nutrition interventions can be personalised across diverse population groups and different settings. Such approaches can benefit from evolving technologies embedding genomics, metabolomics, proteomics, and transcriptomics, together with behavioural reduction strategies such as addressing sedentary behaviour. This narrative review focusses on describing personalised lifestyle prevention approaches, which address different
population needs and environmental settings. These methods can be better directed towards translating T2DM interventions and laboratory trials into sustainable, healthy behaviours, and help form personalised lifestyle T2DM prevention guidelines.
Has Diamond Grinding Been A Cost Effective Pavement Preservation Treatment In Australia?
Authors: Vorobieff George
Published: November 2021

Australia introduced conventional longitudinal diamond grinding of highway concrete pavements in 2009 with the purchase of two "4‐foot" highway grinding machines by two contractors. The availability of these machines in Australia has enabled contractors to improve ride quality of new pavements, rather than accept a deduction to the tendered rate for the supply and placement of concrete pavement. Grinding of new concrete base is permitted up to an IRI of 3.5 m/km, thereby reducing the need to remove and replace concrete pavement which met the specified thickness, strength and density, but not ride quality. More importantly, with the introduction of the grinding machines, asset managers have the opportunity to use diamond grinding to treat existing concrete pavements that have a rough ride, or when the textured surface no longer meets specified levels for skid resistance. Although the primary use of diamond grinding was to improve ride quality of new and existing concrete pavements, it has also been used to: treat stepping across transverse contraction joints in PCP, improve skid resistance at roundabouts, improve both ride quality and texture for JRCP pavements (greater than 40 years of age) with a thin wearing course and spalling in the asphalt at transverse joints. The above treatments to concrete pavement allow asset preservation and avoid high reconstruction costs. The Austroads concrete pavement design procedure is based on the PCA design method and road smoothness is not a design parameter, unlike the USA approach to concrete pavement design where ride quality is a design input. There is still much work to be done to convince asset managers in Australia that the removal of the high areas of a concrete pavement to smooth the surface, reduces the dynamic wheel loading and minimises accumulated fatigue stress in the concrete. This paper reviews the last 10 years of diamond grinding projects and the success of this pavement preservation treatment for new and existing urban and rural concrete pavements in Australia. Recommendations to reduce the cost of diamond grinding concrete pavements and extend the use of this treatment are also provided.
Quality Assurance Methods Applied For Exposed-Aggregate Concrete Pavement Construction
Authors: Glinicki Adam , Glinicki Michal
Published: November 2021

The exposed aggregate pavement technology for construction of concrete highways is used in European countries, including Poland, mostly for heavy trafficked roads. It is mainly a two-lift slip-form technology with a special treatment of the top surface after the final smoothing operation. This is a demanding technology that leaves a little margin for mistakes. When properly done the pavement layer with exposed aggregates ensures designed skid resistance for vehicle wheels even in adverse weather conditions without excessive traffic noise. The challenge is to provide its cost-effective long term performance including both the adequate roughness and the desired smoothness of the pavement. The paper presents tools and methods for construction quality assurance specific for exposed aggregate concrete pavements. Required monitoring of the stability of concrete mix properties is discussed. The importance of concrete curing is analyzed in respect to the long term durability in wet-freeze regions with heavy use of deicing salts. Macrotexture assessment at the early stage of pavement construction is seen as the key factor for assurance of the proper skidding resistance. Local evaluation of smoothness is also a useful approach to assure the target IRI. Examples of quality assurance efforts applied on concrete highways recently constructed in Poland are presented.
Nomos and Platonism in More's Utopia
Authors: Avery Joshua
Published: November 2021

This essay, following an existing train of scholarship working to make sense of the Platonic connection to Utopia, argues for nomos as a useful angle in furthering this understanding. Raphael's approach to politics combines with the Utopian social system to suggest a highly Platonic vision of nomos, whereby social norms are absorbed into an essentialized nature, stripped of all arbitrariness and therefore, ostensibly, perfectly rational. The result is a sterile regime that fails to acknowledge the whimsical elements necessary to the human soul and therefore also necessary to actual human societies.
An O(log2N) Fully-Balanced Resampling Algorithm for Particle Filters on Distributed Memory Architectures
Authors: Varsi Alessandro , Maskell Simon , Spirakis Paul G.
Published: November 2021

Resampling is a well-known statistical algorithm that is commonly applied in the context of Particle Filters (PFs) in order to perform state estimation for non-linear non-Gaussian dynamic models. As the models become more complex and accurate, the run-time of PF applications becomes increasingly slow. Parallel computing can help to address this. However, resampling (and, hence, PFs as well) necessarily involves a bottleneck, the redistribution step, which is notoriously challenging to parallelize if using textbook parallel computing techniques. A state-of-the-art redistribution takes O((log2N)2) computations on Distributed Memory (DM) architectures, which most supercomputers adopt, whereas redistribution can be performed in O(log2N) on Shared Memory (SM) architectures, such as GPU or mainstream CPUs. In this paper, we propose a novel parallel redistribution for DM that achieves an O(log2N) time complexity. We also present empirical results that indicate that our novel approach outperforms the O((log2N)2) approach.
Computing the Atom Graph of a Graph and the Union Join Graph of a Hypergraph
Authors: Berry Anne , Simonet Geneviève
Published: November 2021

The atom graph of a graph is a graph whose vertices are the atoms obtained by clique minimal separator decomposition of this graph, and whose edges are the edges of all possible atom trees of this graph. We provide two efficient algorithms for computing this atom graph, with a complexity in O(min(nωlogn,nm,n(n+m¯)) time, where n is the number of vertices of G, m is the number of its edges, m¯ is the number of edges of the complement of G, and ω, also denoted by α in the literature, is a real number, such that O(nω) is the best known time complexity for matrix multiplication, whose current value is 2,3728596. This time complexity is no more than the time complexity of computing the atoms in the general case. We extend our results to α-acyclic hypergraphs, which are hypergraphs having at least one join tree, a join tree of an hypergraph being defined by its hyperedges in the same way as an atom tree of a graph is defined by its atoms. We introduce the notion of union join graph, which is the union of all possible join trees; we apply our algorithms for atom graphs to efficiently compute union join graphs.
Enhancing the Interlaminar Fracture Toughness and AO Resistance of CFRPs by Using Phosphorus-Containing Polymer/PEC-K Bifunctional Film
Authors: Guo Miaocai
Published: November 2021

A new attempt to use a bifunctional interleaf for developing a novel structure–function-integrated composite with simultaneously improved interlaminar fracture toughness and atomic oxygen resistance was studied. The toughening mechanism and the atomic oxygen erosion property of the delaminated surfaces of the composites were examined. The bifunctional interleaf was prepared by blending a phosphorus-containing polymer and a thermoplastic polymer. After being interleaved, the mode I and mode II fracture toughness increased by 8.2% and 23.7% compared to the control sample, respectively. The toughness gains are much smaller than that of the only thermoplastic film-toughened composite because of the relative brittleness of the blend film. The atomic oxygen erosion rates of the mode I and mode II delamination surfaces decreased by 45.3% and 31.3% compared with the control, respectively. The carbon fibers on the irradiation surfaces are protected by a layer of phosphine oxide to prevent further erosion, and they were much less eroded, particularly for the mode I surface. In comparison, the erosion rates of the mode I and mode II surfaces of the toughened-only composite significantly increased by 83.6% and 107.2%, respectively, and the carbon fibers are seriously eroded.
Modeling the Essential Oil and Trans-Anethole Yield of Fennel (Foeniculum vulgare Mill. var. vulgare) by Application Artificial Neural Network and Multiple Linear Regression Methods
Authors: Sabzi-Nojadeh Mohsen , Niedbała Gniewko , Younessi-Hamzekhanlu Mehdi , Aharizad Saeid , Esmaeilpour Mohammad , Abdipour Moslem , Kujawa Sebastian , Niazian Mohsen
Published: November 2021

Foeniculum vulgare Mill. (commonly known as fennel) is used in the pharmaceutical, cosmetic, and food industries. Fennel widely used as a digestive, carminative, galactagogue and diuretic and in treating gastrointestinal and respiratory disorders. Improving low heritability traits such as essential oil yield (EOY%) and trans-anethole yield (TAY%) of fennel by direct selection does not result in rapid gains of EOY% and TAY%. Identification of high-heritable traits and using efficient modeling methods can be a beneficial approach to overcome this limitation and help breeders select the most advantageous traits in medicinal plant breeding programs. The present study aims to compare the performance of the artificial neural network (ANN) and multilinear regression (MLR) to predict the EOY% and TAY% of fennel populations. Stepwise regression (SWR) was used to assess the effect of various input variables. Based on SWR, nine traits—number of days to 50% flowering (NDF50%), number of days to maturity (NDM), final plant height (FPH), number of internodes (NI), number of umbels (NU), seed yield per square meter (SY/m2), number of seeds per plant (NS/P), number of seeds per umbel (NS/U) and 1000-seed weight (TSW)—were chosen as input variables. The network with Sigmoid Axon transfer function and two hidden layers was selected as the final ANN model for the prediction of EOY%, and the TanhAxon function with one hidden layer was used for the prediction of TAY%. The results revealed that the ANN method could predict the EOY% and TAY% with more accuracy and efficiency (R2 of EOY% = 0.929, R2 of TAY% = 0.777, RMSE of EOY% = 0.544, RMSE of TAY% = 0.264, MAE of EOY% = 0.385 and MAE of TAY% = 0.352) compared with the MLR model (R2 of EOY% = 0.553, R2 of TAY% = 0.467, RMSE of EOY% = 0.819, RMSE of TAY% = 0.448, MAE of EOY% = 0.624 and MAE of TAY% = 0.452). Based on the sensitivity analysis, SY/m2, NDF50% and NS/P were the most important traits to predict EOY% as well as SY/m2, NS/U and NDM to predict of TAY%. The results demonstrate the potential of ANNs as a promising tool to predict the EOY% and TAY% of fennel, and they can be used in future fennel breeding programs.
The Promise of Hyperspectral Imaging for the Early Detection of Crown Rot in Wheat
Authors: Xie Yiting , Plett Darren , Liu Huajian
Published: November 2021

Crown rot disease is caused by Fusarium pseudograminearum and is one of the major stubble-soil fungal diseases threatening the cereal industry globally. It causes failure of grain establishment, which brings significant yield loss. Screening crops affected by crown rot is one of the key tools to manage crown rot, because it is necessary to understand disease infection conditions, identify the severity of infection, and discover potential resistant varieties. However, screening crown rot is challenging as there are no clear visible symptoms on leaves at early growth stages. Hyperspectral imaging (HSI) technologies have been successfully used to better understand plant health and disease incidence, including light absorption rate, water and nutrient distribution, and disease classification. This suggests HSI imaging technologies may be used to detect crown rot at early growing stages, however, related studies are limited. This paper briefly describes the symptoms of crown rot disease and traditional screening methods with their limitations. It, then, reviews state-of-art imaging technologies for disease detection, from color imaging to hyperspectral imaging. In particular, this paper highlights the suitability of hyperspectral-based screening methods for crown rot disease. A hypothesis is presented that HSI can detect crown-rot-infected plants before clearly visible symptoms on leaves by sensing the changes of photosynthesis, water, and nutrients contents of plants. In addition, it describes our initial experiment to support the hypothesis and further research directions are described.
Characterization of Beekeepers and Their Activities in Seven European Countries
Authors: Guiné Raquel P. F. , Mesquita Sónia , Oliveira Jorge , Coelho Catarina , Costa Daniela Teixeira , Correia Paula , Correia Helena Esteves , Dahle Bjørn , Oddie Melissa , Raimets Risto , Karise Reet , Tourino Luis , Basile Salvatore , Buonomo Emilio , Stefanic Ivan , Costa Cristina A.
Published: November 2021

Beekeeping is an ancient activity that is gaining interest among practitioners and society in general. It is as an activity with positive impacts in the environmental, social and economic spheres, with the potential to reconnect these dimensions and contribute to sustainable development. Thus, it is important to determine the profiles of beekeepers across the world, and to understand the main social, economic or ecological drivers that shape their activities. Hofstede cross-cultural dimensions were used to better explain differences between countries. A survey was undertaken of beekeepers in different countries (Croatia, Estonia, Finland, Italy, Norway, Portugal, and Spain) in the native language of each nation. A total of 313 questionnaires, using an online platform or in paper form, was delivered face-to-face during training or dissemination events in 2019. Norway and Finland were the countries with the highest percentage of respondents with a university degree (>80%), while Spain (42%) and Croatia (48%) presented the lowest percentages. Most participants were experienced beekeepers (59% had more than five years of experience) with more than 50 colonies. With the exception of Italy, beekeeping appears to be a hobby or an additional professional activity. The main beekeeping products for these beekeepers were honey, wax, colonies and propolis, with an average honey production per season of 24.5 kg/hive. Crossing socio-demographic characteristics and Hofstede cross-cultural dimensions showed a relation among countries with higher Power Distance Index (PDI; this value expresses how society accepts and expects a certain inequality of power) and lower annual income and educational level (Croatia, Portugal, and Spain). A strong correlation appeared between Masculinity Femininity Index (MFI; this value refers to gender effects in society, with feminine societies meaning that the dominant values are caring for others and quality of life, as opposed to masculine societies, which are driven by competition, achievement and success) and age, income and education, with Norway presenting the most feminine society, with more educated and older beekeepers. The Uncertainty Aversion Index (UAI; this value explains how members of a society feel when dealing with unknown situations) was strongly associated with education. The results showed that increasing beekeepers’ income will contribute to balancing the distribution of power among members of society, and that this might be achieved by training, especially in Croatia, Portugal, Spain, and Italy.
Impacts of Farming Layer Constructions on Cultivated Land Quality under the Cultivated Land Balance Policy
Authors: Kang Long , Zhao Rui , Wu Kening , Huang Qin , Zhang Sicheng
Published: November 2021

Cultivated Land Balance Policy (CLBP) has led to the “better land occupied and worse land supplemented” program. At the same time, the current field-scale cultivated land quality (CLQ) evaluation cannot meet the work requirements of the CLBP. To this end, this study selected 24 newly added farmland in Fuping County and performed eight different high quality farming layer construction experiments to improve the CLQ. A new comprehensive model was constructed on a field scale to evaluate the CLQ using different tests from multi-dimensional perspectives of soil fertility, engineering, environment, and ecology, and to determine the best test mode. The results showed that after the test, around 62% of the cultivated land improved by one level, and the average cultivated land quality level and quality index of the test area increased by 0.63 and 30.63, respectively. The treatment of “woody peat + rotten crop straw + biostimulation regulator II + conventional fertilization” had the best effect on the improvement of organic matter, soil aggregates, and soil microbial activity, and was the best treatment method. In general, application of soil amendments, such as woody peat when constructing high quality farmland, could quickly improve CLQ, and field-scale CLQ evaluation model constructed from a multi-dimensional perspective could accurately assess the true quality of farmland and allow managers to improve and manage arable land resources under CLBP.
Phenotyping and Identification of Reduced Height (Rht) Alleles (Rht-B1b and Rht-D1b) in a Nepali Spring Wheat (Triticum aestivum L.) Diversity Panel to Enable Seedling Vigor Selection
Authors: Khadka Kamal , Kaviani Mina , Raizada Manish N. , Navabi Alireza
Published: November 2021

Nepal is facing more intense early-season drought stress associated with climate change. The introgression of reduced height (Rht) alleles to enable stem dwarfism in bread wheat (Triticum aestivum L.) inadvertently reduced coleoptile length and growth plasticity in seedlings, making improved varieties less suitable for deep seeding; these alleles may have also reduced seedling root length. Therefore, with the long-term objective of breeding wheat for early-season drought stress, a Nepali spring wheat panel was evaluated to assess allelic variation at the most common dwarfing-associated loci (Rht-B1, Rht-D1) and their impact on coleoptile/seedling root traits, and to identify accessions with longer and/or more GA-responsive coleoptiles as parents for future breeding. Here, Kompetitive Allele Specific PCR (KASP) was used to genotype accessions. The panel was phenotyped using the cigar-roll method in the presence/absence of GA3. Plant height was measured under field conditions. The results showed that Nepali landraces had a significantly higher frequency of the non-dwarfing allele Rht-B1a. The dwarfing alleles Rht-B1b and Rht-D1b had negative effects on coleoptile length but positive effects on the length of the longest seedling root. However, 40 potential semi-dwarf accessions (possessing Rht-B1b and/or Rht-D1b alleles) with long and/or more plastic coleoptiles suited for deep sowing were identified. This included 12 accessions that exhibited significant changes in coleoptile length in response to GA3 treatment.
The Melon Zym Locus Conferring Resistance to ZYMV: High Resolution Mapping and Candidate Gene Identification
Authors: Adler-Berke Nastacia , Goldenberg Yitzchak , Brotman Yariv , Kovalski Irina , Gal-On Amit , Doniger Tirza , Harel-Beja Rotem , Troadec Christelle , Bendahmane Abdelhafid , Pitrat Michel , Dogimont Catherine , Katzir Nurit , Perl-Treves Rafael
Published: November 2021

Zucchini yellow mosaic virus (ZYMV; potyviridae) represents a major pathogen of Cucurbitaceae crops. ZYMV resistance in melon PI 414723 is conditioned by a dominant allele at the Zym locus. This resistant accession restricts viral spread and does not develop mosaic symptoms, but necrosis sometimes develops in response to inoculation. In previous studies, Zym has been mapped to linkage group II of the melon genetic map. In the present study, positional cloning of the locus was undertaken, starting from the CM-AG36 SSR marker at approximately 2 cm distance. We utilized five mapping populations that share the same resistant parent, PI 414723, and analyzed a total of 1630 offspring, to construct a high-resolution genetic map of the Zym locus. Two melon BAC libraries were used for chromosome walking and for developing new markers closer to the resistance gene by BAC-end sequencing. A BAC contig was constructed, and we identified a single BAC clone, from the ZYMV susceptible genotype MR-1, that physically encompasses the resistance gene. A second clone was isolated from another susceptible genotype, WMR 29, and the two clones were fully sequenced and annotated. Additional markers derived from the sequenced region delimited the region to 17.6 kb of a sequence that harbors a NAC-like transcription factor and, depending on the genotype, either two or three R-gene homologs with a CC-NBS-LRR structure. Mapping was confirmed by saturating the map with SNP markers using a single mapping population. The same region was amplified and sequenced also in the ZYMV resistant genotype PI 414723. Because numerous polymorphic sites were noted between genotypes, we could not associate resistance with a specific DNA polymorphism; however, this study enables molecular identification of Zym and paves the way to functional studies of this important locus.
Ion Channel and Ubiquitin Differential Expression during Erythromycin-Induced Anhidrosis in Foals
Authors: Patterson Rosa Laura , Mallicote Martha F. , MacKay Robert J. , Brooks Samantha A.
Published: November 2021

Macrolide drugs are the treatment of choice for Rhodococcus equi infections, despite severe side-effects temporary anhidrosis as a. To better understand the molecular biology leading to macrolide induced anhidrosis, we performed skin biopsies and Quantitative Intradermal Terbutaline Sweat Tests (QITSTs) in six healthy pony-cross foals for three different timepoints during erythromycin administration—pre-treatment (baseline), during anhidrosis and post-recovery. RNA sequencing of biopsies followed by differential gene expression analysis compared both pre and post normal sweating timepoints to the erythromycin induced anhidrosis episode. After Bonferroni correction for multiple testing, 132 gene transcripts were significantly differentially expressed during the anhidrotic timepoint. Gene ontology analysis of the full differentially expressed gene set identified over-represented biological functions for ubiquitination and ion-channel function, both biologically relevant to sweat production. These same mechanisms were previously implicated in heritable equine idiopathic anhidrosis and sweat gland function and their involvement in macrolide-induced temporary anhidrosis warrants further investigation.
Comparison of Threshold and Tolerance Nociceptive Withdrawal Reflexes in Horses
Authors: Mühlemann Selina , Leandri Massimo , Risberg Åse Ingvild , Spadavecchia Claudia
Published: November 2021

The nociceptive withdrawal reflex (NWR) is used to investigate nociception in horses. The NWR threshold is a classical model endpoint. The aims of this study were to determine NWR tolerance and to compare threshold and tolerance reflexes in horses. In 12 horses, the NWR was evoked through electrical stimulation of the digital nerve and recorded via electromyography from the deltoid. Behavioral reactions were scored from 0 to 5 (tolerance). First, the individual NWR threshold was defined, then stimulation intensity was increased to tolerance. The median NWR threshold was 7.0 mA, whereas NWR tolerance was 10.7 mA. Upon visual inspection of the records, two main reflex components R1 (median latency 44 ms) and R2 (median latency 81 ms) were identified at threshold. Increasing stimulation intensity to tolerance led to a significant increase in the amplitude and duration of R1 and R2, whereas their latency decreased. At tolerance, a single burst of early, high-amplitude reflex activity, with a median latency of 39 ms, was detected in 15 out of 23 stimulations (65%). The results of this study suggest that (1) it is feasible to determine NWR tolerance in horses and (2) high-intensity stimuli initiate ultrafast bursts of reflex activity, which is well known in practice and has now been quantified using the NWR model.
Development of the Dog Attachment Insecurity Screening Inventory (D-AISI): A Pilot Study on a Sample of Female Owners
Authors: Riggio Giacomo , Noom Marc , Gazzano Angelo , Mariti Chiara
Published: November 2021

To date, the Strange Situation Procedure is the only tool available to investigate the quality of the dog’s attachment bond towards the owner. This study aimed to adapt a parent-report scale, named the Attachment Insecurity Screening Inventory (AISI) 6–12, originally designed to assess 6- to 12-year-old children’s attachment insecurity, to dog–owner dyads and assess measures of consistency and validity. The online questionnaire was completed by 524 female dog owners. Principal component analysis (PCA) revealed five components named, respectively, “physical contact”, “control”, “separation anxiety”, “owner as emotional support”, and “owner as a source of positive emotion”. Because of the three-factor structure of the original AISI, a PCA with a pre-fixed set of three factors was also performed. The resulting subscales mirrored the ones found for the original scale (i.e., ambivalent, avoidant, and disorganized), although four items did not fit the model. Internal reliability appeared to be satisfying for the ambivalent and the disorganized subscales, and good for the avoidant subscale. The theoretical background and the results of this study suggest that the three-dimensional model represents a better solution for the interpretation of the Dog Attachment Insecurity Screening Inventory (D-AISI). Although promising, this scale requires refinement and assessment of additional validity measures.
Essential Oil Delivery Route: Effect on Broiler Chicken’s Growth Performance, Blood Biochemistry, Intestinal Morphology, Immune, and Antioxidant Status
Authors: Oladokun Samson , MacIsaac Janice , Rathgeber Bruce , Adewole Deborah
Published: November 2021

This study evaluated the effect of an essential oil blend and its delivery routes on broiler chicken growth performance, blood biochemistry, intestinal morphology, and immune and antioxidant status. Eggs were incubated and allotted to 3 groups: non-injected group, in ovo saline group, and in ovo essential oil group. On day 18 of incubation, essential oil in saline or saline alone was injected into the amnion. At hatch, chicks were assigned to post-hatch treatment combinations (1) in ovo essential oil + in-water essential oil (in ovo + in-water EO); (2) in ovo essential oil (in ovo EO); (3) in ovo saline; (4) in-water essential oil; (5) in-feed antibiotics (Bacitracin methylene disalicylate) and (6) a negative control (NC; corn-wheat-soybean diet) in 8 replicate cages (6 birds/cage) and raised for 28 day. The in ovo EO group reduced (p < 0.05) chick length and hatchability, all groups recorded no difference in growth performance at 0–28 day. The in ovo + in-water EO treatment reduced (p < 0.05) blood creatine kinase and aspartate aminotransferase levels whilst increasing (p < 0.05) total antioxidant capacity in birds. The in ovo + in-water delivery of EO might represent a potential antibiotic reduction strategy for the poultry industry but more research is needed to address the concern of reduced hatchability.
Probiotics in Rabbit Farming: Growth Performance, Health Status, and Meat Quality
Authors: Mancini Simone , Paci Gisella
Published: November 2021

The rabbit’s complex microbiota of the gastrointestinal tract (GIT) plays a critical role in feed digestion, in vitamin production, in fermentative activity with production of volatile fatty acids, and stimulation of immune response, as well as in the infection defence against pathogens and countering environmental stresses. To prevent digestive disorders of this fragile ecosystem, rabbit breeders adopt suitable diets supplemented with additives such as probiotics. Probiotics can interact with the host and with the other microflora leading to an increased health status. A review on the effects of probiotics on rabbit growth performance, health status, and meat quality was conducted to reduce the incidence of digestive diseases and enhance productive performance. Some authors observed that the supplementation of probiotics to the diet improved feed conversion ratio and growth and digestion coefficients, while other authors reported a lack of effect on the live performance. Benefits derived from the use of probiotics were observed on the mortality and the morbidity. The studies performed, to evaluate the effects of probiotic supplementation in diets on the immune response, showed variations in the results. Some authors reported no significant effect on haematological parameters, such as total protein, immunoglobulins, and IgG, while others observed a significant increase or decrease of the same parameters. Most of the research reported significant modifications of intestinal morphology and positive effects on the GIT microbiota, supporting the host’s natural defences. Regarding the carcass and meat quality, the studies reported only partial and opposing results.
Global Studies of the Host-Parasite Relationships between Ectoparasitic Mites of the Family Syringophilidae and Birds of the Order Columbiformes
Authors: Kaszewska-Gilas Katarzyna , Kosicki Jakub Ziemowit , Hromada Martin , Skoracki Maciej
Published: November 2021

The quill mites belonging to the family Syringophilidae (Acari: Prostigmata: Cheyletoidea) are obligate ectoparasites of birds. They inhabit different types of the quills, where they spend their whole life cycle. In this paper, we conducted a global study of syringophilid mites associated with columbiform birds. We examined 772 pigeon and dove individuals belonging to 112 species (35% world fauna) from all zoogeographical regions (except Madagascan) where Columbiformes occur. We measured the prevalence (IP) and the confidence interval (CI) for all infested host species. IP ranges between 4.2 and 66.7 (CI 0.2–100). We applied a bipartite analysis to determine host–parasite interaction, network indices, and host specificity on species and whole network levels. The Syringophilidae–Columbiformes network was composed of 25 mite species and 65 host species. The bipartite network was characterized by a high network level specialization H2′ = 0.93, high nestedness N = 0.908, connectance C = 0.90, and high modularity Q = 0.83, with 20 modules. Moreover, we reconstructed the phylogeny of the quill mites associated with columbiform birds on the generic level. Analysis shows two distinct clades: Meitingsunes + Psittaciphilus, and Peristerophila + Terratosyringophilus.
Intestinal Helminth Communities of Grey Partridge Perdix perdix and Common Pheasant Phasianus colchicus in Poland
Authors: Rząd Izabella , Stapf Agata , Kornaś Sławomir Adam , Dzika Ewa , Sałamatin Rusłan , Kaczmarek Adam , Kowal Jerzy , Wajdzik Marek , Zalewski Kazimierz
Published: November 2021

The aim of this study was to describe the morphology and means of identification of helminths in native partridges (65) and introduced pheasants (32) in Poland and to determine the level of intestinal infection of these birds by helminths using parasitological and ecological indices. The birds were acquired during the hunting season in the years 2015–2017. Nematodes, Capillaria phasianina, cestodes, Railietina friedbergeri, and one trematode, Brachylaima sp. were recorded for the first time in partridges in Poland. Our findings indicate that parasites are more prevalent in pheasants (prevalence 70.4%) than in partridges (prevalence 50.0%). The component community and infracommunity of parasites of partridges are more diverse (Simpson’s diversity index: 0.63 and mean Brillouin diversity index: 0.10 ± 0.17) and less dominated by a single parasite species (Capillaria sp., Berger-Parker dominance index: 0.53) than the pheasant parasite community (Simpson’s diversity index: 0.07, mean Brillouin diversity index: 0.005 ± 0.02, dominant species Heterakis gallinarum, Berger-Parker dominance index: 0.96). There were statistically significant differences between partridges and pheasants in the Brillouin diversity index and in the prevalence of Heterakis gallinarum (55.6% in pheasants vs. 19.0 in partridges). There were significant differences between wild and farmed partridges in the prevalence of infection by Capillaria sp. (4.3% vs. 37.5%) and H. gallinarum (39.1 vs. 6.2%). In conclusion, the pheasant was shown to be a reservoir, carrier, and shedder of nematodes, which may increase the risk of infection in partridges.
Habitat Selection and Activity Patterns of Japanese Serows and Sika Deer with Currently Sympatric Distributions
Authors: Seki Yoshikazu , Hayama Shin-ichi
Published: November 2021

The Japanese serow (Capricornis crispus) and sika deer (Cervus nippon) in Japan are usually allopatric. However, a recent expansion in the distribution range of sika deer, combined with an increase in abundance, has resulted in an overlap of the distribution ranges of the two species. We examined the habitat selection and activity patterns of Japanese serows and sika deer using camera traps placed at 83 sites within a 210 km2 study area, where the distribution range of these two species has recently overlapped. Although both species were photographed throughout the study area, we observed a low spatial overlap between them. The Japanese serows selected steep slopes, and the sika deer selected areas far away from human settlements. In addition, the Japanese serows and sika deer exhibited temporal partitioning with diurnal and crepuscular activity patterns, respectively. The observed partitioning could be explained by differences in their species-specific habitat selections, rather than competition, because the photographic capture rate of the Japanese serows was not affected by that of the sika deer and vice versa. These partitioning behaviors are likely to reduce the rate of encounters between the two ungulates, which enables their coexistence considering the sika deer density remains low.
Characterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars
Authors: Mattison Christopher P. , Vant-Hull Barry , de Castro Ana Cecilia Ribeiro , Chial Heidi J. , Bren-Mattison Yvette , Bechtel Peter J. , de Brito Edy Sousa
Published: November 2021

Ana o 3 is an immuno-dominant cashew nut allergen. Four monoclonal antibodies to Ana o 3 (2H5, 6B9C1, 19C9A2, and 5B7F8) were characterized by ELISA and in silico modeling. The 2H5 antibody was the only antibody specific for cashew nut extract. In addition to cashew nut extract, the 6B9C1 and 19C9A2 antibodies recognized pistachio extract, and the 5B7F8 recognized pecan extract. All four antibodies recognized both recombinant Ana o 3.0101 and native Ana o 3. ELISA assays following treatment of purified Ana o 3 with a reducing agent indicated that the 6B9C1 and 19C9A2 antibodies likely recognize conformational epitopes, while the 2H5 and 5B7F8 antibodies likely recognize linear epitopes. In silico modeling predicted distinct epitopes for each of the anti-Ana o 3 antibodies. Screening extracts from 11 Brazilian cashew nut cultivars using all four antibodies showed slight differences in Ana o 3 bindings, demonstrating that these antibodies could identify cultivars with varying allergen content.
Comprehensive Evaluation of the Antibacterial and Antifungal Activities of Carlina acaulis L. Essential Oil and Its Nanoemulsion
Authors: Rosato Antonio , Barbarossa Alexia , Mustafa Ahmed M. , Bonacucina Giulia , Perinelli Diego Romano , Petrelli Riccardo , Maggi Filippo , Spinozzi Eleonora
Published: November 2021

Plants are considered to be an excellent source of new compounds with antibiotic activity. Carlina acaulis L. is a medicinal plant whose essential oil (EO) is mainly characterized by the polyacetylene carlina oxide, which has antimicrobial properties. The aim of this study was to evaluate the antimicrobial and antifungal activities of C. acaulis EO, carlina oxide, and nanoemulsion (NE) containing the EO. The EO was obtained through plant roots hydrodistillation, and carlina oxide was purified from it through silica gel column chromatography. The NE containing C. acaulis EO was prepared with the high-pressure homogenization method, and the minimum inhibitory concentration (MIC) was determined against several bacterial and fungal strains for all the C. acaulis-derived products. The latter resulted active versus all the screened Gram-positive bacterial strains and also on all the fungal strains with low MIC values. For yeast, the EO and carlina oxide showed good MIC values. The EO-NE demonstrated a better activity than the pure EO on all the tested bacterial and fungal strains. The results suggest that C. acaulis-derived products could be potential candidates for the development of natural antibacterial and antifungal agents.
Alternative and Complementary Therapies against Foodborne Salmonella Infections
Authors: Ghaly Mohamed F. , Nasr Zahraa M. , Abousaty Amira I. , Seadawy Hanan G. , Shaheen Mohamed A. A. , Albogami Sarah , Al-Sanea Mohammad M. , Bendary Mahmoud M.
Published: November 2021

The limitations in the therapeutic options for foodborne pathogens lead to treatments failure, especially for multidrug-resistant (MDR) Salmonella sp., worldwide. Therefore, we aimed to find alternative and complementary therapies against these resistant foodborne pathogens. Out of 100 meat products samples, the prevalence rate of salmonella was 6%, serotyped only as S. Typhimurium and S. Enteritidis. According to the antibiotic susceptibility assays, the majority of our isolates were MDR and susceptible to cefotaxime. Out of the 13 tested plant extracts, five only showed an inhibition zone in the range of 8–50 mm against both serotypes. Based on their promising activity, the oily extract of cinnamon and aqueous extract of paprika represented the highest potency. Surprisingly, a significant synergistic effect was detected between cinnamon oil and cefotaxime. Depending on Gas Chromatography/Mass Spectrometry (GC-MS), the antimicrobial activity of cinnamon oil was attributed to four components including linalool, camphor, (Z)-3-Phenylacrylaldehyde and its stereoisomer 2-Propenal-3-phenyl. The anti-virulence activities of these compounds were confirmed on the basis of computational molecular docking studies. Accordingly, we recommended the use of cinnamon oil as a food additive to fight the resistant foodborne pathogens. Additionally, we confirmed its therapeutic uses, especially when co-administrated with other antimicrobial agents.
New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives
Authors: Kovačević Zorana , Kladar Nebojša , Čabarkapa Ivana , Radinović Miodrag , Maletić Milan , Erdeljan Mihajlo , Božin Biljana
Published: November 2021

Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.
Knowledge, Attitudes, Perception and Reported Practices of Healthcare Providers on Antibiotic Use and Resistance in Pregnancy, Childbirth and Children under Two in Lao PDR: A Mixed Methods Study
Authors: Sychareun Vanphanom , Sihavong Amphoy , Machowska Anna , Onthongdee Xanded , Chaleunvong Kongmany , Keohavong Bounxou , Eriksen Jaran , Hanson Claudia , Vongsouvath Manivanh , Marrone Gaetano , Brauner Annelie , Mayxay Mayfong , Kounnavong Sengchanh , Lundborg Cecilia Stålsby
Published: November 2021

Background: Overuse and misuse of antibiotics contribute unnecessarily to antibiotic resistance (ABR), and are thereby global health threats. Inappropriate prescriptions of antibiotics during pregnancy, delivery and early childhood are widespread across the world. This study aimed to assess knowledge, attitudes, and reported practices of healthcare providers (HCPs) and to explore their perceptions regarding antibiotic use and ABR related to pregnancy, childbirth, and children under two in Lao PDR. Methods: This is a mixed methods study with data collection in 2019 via structured interviews among 217 HCPs (medical doctors/assistant doctors, midwives/nurses, pharmacists/assistant pharmacists and drug sellers), who prescribed/dispensed antibiotics in one rural and one urban district in Vientiane province and individual qualitative interviews with 30 HCPs and stakeholders. Results: Of the HCPs, 36% had below average knowledge regarding antibiotic use and ABR, and 67% reported prescribing antibiotics for uncomplicated vaginal delivery. Half of the HCPs did not believe that their prescribing contributed to ABR, and only 9% had participated in antibiotic education. Conclusion: A substantial number of HCPs had suboptimal knowledge and prescribed antibiotics unnecessarily, thereby contributing to ABR. Continuous education and regular supervision of HCPs is recommended to improve the use of antibiotics related to pregnancy, childbirth, and young children.
Isolation of Human Lineage, Fluoroquinolone-Resistant and Extended-β-Lactamase-Producing Escherichia coli Isolates from Companion Animals in Japan
Authors: Sato Toyotaka , Yokota Shin-ichi , Tachibana Tooru , Tamai Satoshi , Maetani Shigeki , Tamura Yutaka , Horiuchi Motohiro
Published: November 2021

An increase in human and veterinary fluoroquinolone-resistant Escherichia coli is a global concern. In this study, we isolated fluoroquinolone-resistant E. coli isolates from companion animals and characterized them using molecular epidemiological analysis, multiplex polymerase chain reaction to detect E. coli ST131 and CTX-M type extended-spectrum β-lactamases (ESBL), and multi-locus sequence typing analysis. Using plain-CHROMagar ECC, 101 E. coli isolates were isolated from 34 rectal swabs of dogs and cats. The prevalence of resistance to fluoroquinolone and cefotaxime was 27.7% and 24.8%, respectively. The prevalence of fluoroquinolone-resistant isolates (89.3%) was higher when CHROMagar ECC with CHROMagar ESBL supplement was used for E. coli isolation. The prevalence of cefotaxime resistance was also higher (76.1%) when 1 mg/L of ciprofloxacin-containing CHROMagar ECC was used for isolation. The cefotaxime-resistant isolates possessed CTX-M type β-lactamase genes (CTX-M-14, CTX-M-15, or CTX-M-27). Seventy-five percent of fluoroquinolone-resistant isolates were sequence types ST131, ST10, ST1193, ST38, or ST648, which are associated with extensive spread in human clinical settings. In addition, we isolated three common fluoroquinolone-resistant E. coli lineages (ST131 clade C1-M-27, C1-nM27 and ST2380) from dogs and their respective owners. These observations suggest that companion animals can harbor fluoroquinolone-resistant and/or ESBL-producing E. coli, in their rectums, and that transmission of these isolates to their owners can occur.