bluuebunny/crossref_metadata_2025 huggingface dataset:
The design and synthesis of benzylpiperazine-based edaravone derivatives and their neuroprotective activities
Authors: Gao Mengjie , Ma Shuangyan , Xu Tong , Jiang Nan , Xu Yi , Zhong Yan , Wu Bin
Published: December 2022

New edaravone derivatives containing a benzylpiperazine moiety are designed and synthesized. The structures are characterized by 1H NMR, 13C NMR, and high-resolution mass spectrometry. The potential neuroprotective activities of the target compounds are evaluated in differentiated rat pheochromocytoma cells (PC12 cells) and in mice subjected to acute cerebral ischemia. Most of the target compounds showed neuroprotective activities both in vivo and in vitro, especially 1-(4-(4-fluorobenzyl) piperazin-1-yl)-2-(4-(5-hydroxy-3-methyl-1 H-pyrazol-1-yl)phenoxy)ethanone and 1-(4-(4-nitrobenzyl)piperazin-1-yl)-2-(4-(5-hydroxy-3-methyl-1 H-pyrazol-1-yl)phenoxy)ethanone, which displayed significant protective effects on cell viability against damage caused by H2O2, and remarkably prolonged the survival time of mice subjected to acute cerebral ischemia and decreased the mortality rate at all doses. These compounds represent lead compounds for the further discovery of neuroprotective agents for treating cerebral ischemic stroke. Molecular docking studies and basic structure–activity relationships are also presented.
Locomotor activities and subjective load differences between professional youth soccer players and professional development loans: A comparative analysis
Authors: Craig Thomas P , Swinton Paul , Barrett Steve , Maughan Patrick
Published: December 2022

This study quantified and compared the weekly locomotor activity and subjective load between elite and development loan youth soccer players registered to a primary club. Development loan players were loaned to a lower-league club and trained part-time with their loan club whilst being available for development fixtures and training with the primary club. Data were collected in 16 squad players and 4 development loan players at loan clubs across a 41 week competitive phase of the 2018/2019 season. Analysis was completed on total distance (m), PlayerLoadTM (au), low intensity running (<14.4 km·h−1, m), running (19.8–24.98 km·h−1, m), sprinting (>24.98 km·h−1, m), accelerations (>2 m·s−2 count) and decelerations (<−2 m·s−2, count). Point estimates for the development loan players consistently showed lower weekly values than squad players for all variables ranging from 5.2% (weekly sRPE) to 16.8% (weekly sprint distance covered). Differences, however, were not found to be statistically significant ( p ≥ 0.07). Variance ranged from 23.6% (weekly distance) to 37.7% (weekly high-intensity accelerations). Although the goals of a development loan are likely to be multifactorial, this is the first study to quantify and compare locomotor activities and subjective loading of players within the development loan environment.
Micro media systems
Authors: Ravn-Højgaard Signe
Published: December 2022

In this article, the concept of “micro media system” is introduced to describe the relations between media and politics in microstates and societies. Drawing on ideas from the literature on democracy in microstates (Benedict, 1967; Corbett and Veenendaal, 2018; Sarapuu and Randma-Liiv, 2020), it is argued that despite their many differences, the smallest media systems share four characteristics linked to their micro size: (1) government domination, (2) high social integration and overlapping role-relationships, (3) multi-functionalism among journalists and media outlets, and (4) dependence on few individuals. These characteristics of micro media systems can weaken the media's position in society vis-à-vis other institutions. Whereas previous research on small media systems (e.g., Puppis, 2009) has considered size as a binary variable, in this article it is argued that the size of media systems is better perceived as a continuous variable. This implies that the characteristics of micro media systems become more pronounced the smaller the media system.
Update on the role of elastography in liver disease
Authors: Ferraioli Giovanna , Roccarina Davide
Published: December 2022

The diagnosis of liver fibrosis and the assessment of its severity are important to provide appropriate management, to determine the prognosis or the need for surveillance. Currently, for fibrosis staging, liver stiffness measurement (LSM) with the shear wave elastography (SWE) techniques is considered a reliable substitute for liver biopsy in several clinical scenarios. Nonetheless, it should be emphasized that stiffness value is a biomarker of diffuse liver disease that must be interpreted taking into consideration anamnesis, clinical and laboratory data. In patients with diffuse liver disease, it is more clinically relevant to determine the likelihood of advanced disease rather than to obtain an exact stage of liver fibrosis using a histologic classification. In this regard, a ‘rule of five’ for LSMs with vibration-controlled transient elastography (VCTE) and a ‘rule of four’ for LSMs with the acoustic radiation force impulse (ARFI)-based techniques have been proposed. In patients with advanced chronic liver disease (CLD), the risk of liver decompensation increases with increasing liver stiffness value. SWE has been proposed as a tool to predict the risk of death or complications in patients with CLD. LSM by VCTE combined with platelets count is a validated non-invasive method for varices screening, with very good results in terms of invasive procedures being spared. ARFI-based techniques also show some promising results in this setting. LSM, alone or combined in scores or algorithms with other parameters, is used to evaluate the risk of hepatocellular carcinoma occurrence. Due to the high prevalence of CLD, screening the population at risk is of interest but further studies are needed.
Histologic healing and factors associated with complete remission following conventional treatment in ulcerative colitis
Authors: Steinsbø Øyvind , Carlsen Arne , Aasprong Ole Gunnar , Aabakken Lars , Tvedt-Gundersen Espen , Bjørkhaug Steinar , Gjerde Rune , Normann Karlsen Lars , Grimstad Tore
Published: December 2022

Background:

Endoscopic and histological activity scores in ulcerative colitis (UC) are associated with clinical outcomes and have become important targets of clinical trials. However, these endpoints have been scarcely investigated in patients receiving only conventional treatment.

Objective:

We aimed to assess the deep and complete remission rates after 3 months of conventional treatment in patients with newly diagnosed UC with moderate to severe endoscopic activity. We also aimed to investigate whether selected clinical and biochemical variables at baseline were associated with complete remission status after 3 months.

Design:

This was a prospective cohort study.

Methods:

Newly diagnosed patients with active UC commencing 5-aminosalicylate, corticosteroid, and/or azathioprine treatment were consecutively included. Clinical, biochemical, endoscopic, and histological data were collected at baseline and after 3 months. Rates of clinical remission (Partial Mayo Score ⩽ 2), mucosal healing (Mayo Endoscopic Score ⩽ 1), and histologic healing (Nancy Index ⩽ 1) were determined. Deep remission was assessed as clinical remission plus mucosal healing and complete remission as deep remission plus histologic healing. Predictors of complete remission were identified by logistic regression.

Results:

A total of 180 patients were included in the study. Deep remission and complete remission occurred in 62.8% and 42.2% of patients, respectively. Thus, of patients in deep remission one-third had persistent histologic activity. Histologic activity in mucosally healed patients was associated with higher symptom scores and faecal calprotectin levels. Of baseline variables, less endoscopic distribution and disease activity showed strongest association with achieving complete remission, and limited distribution in combination with moderate activity gave highest odds for complete remission (odds ratio: 4.1, 95% confidence interval: 7.69–2.18).

Conclusion:

In patients with mucosal healing, persistent histologic activity was a common finding and was associated with increased disease activity. Pancolitis and severe inflammatory activity at baseline were associated with lower complete remission rates.
The Hierarchy of Influences Model, National Culture, Human Development, and Journalism Influences
Authors: Collins Steve J. , Kinnally William , Sandoval Jennifer A.
Published: December 2022

This study examines how social systems level variables may help shape broadcast journalists’ perceptions of the forces influencing their work. We combined Worlds of Journalism Study data with Hofstede's cultural orientations to consider how the levels of the hierarchical influences model may coalesce. In six analyses across four levels, culture was significantly correlated with perceived influences. Our findings support the belief the social systems level is the hegemonic level on which the other levels rest.
Pharmacophore-Based Virtual Screening of Potential SARS-CoV-2 Main Protease Inhibitors from Library of Natural Products
Authors: Wang Jing , Jiang Yu , Wu Yingnan , Yu Hui , Wang Zhanli , Ma Yuheng
Published: December 2022

Background: The SARS-CoV-2 main protease (Mpro) is an attractive target for drug discovery. Methods: A pharmacophore model was built using the three-dimensional (3D) pharmacophore generation algorithm HypoGen in Discovery Studio 2019. The best pharmacophore model was selected for validation using a test set of 24 compounds and was used as a 3D query for further screening of an in-house database of natural compounds. Lipinski's rule of five was used to assess the drug-like properties of the hit compounds. The filtered compounds were then subjected to bioactivity evaluations. The active compounds were docked into the active site of the SARS-CoV-2 Mpro crystal structure (PDB ID: 7D1M). Results: A suitable 3D pharmacophore model, Hypo1, was found to be the best model, consisting of four features (one hydrophobic feature, one hydrogen bond donor, and two hydrogen bond acceptors). Pharmacophore-based virtual screening with Hypo1 as the query to search an in-house database of 34 439 natural compounds resulted in 1502 hits. Among these, 255 compounds satisfied Lipinski's rule of five. The highest ranking 10 compounds were selected for further experimental testing, and one hit (W-7) illustrated inhibitory activity against SARS-CoV-2 Mpro with an IC50 value of 75 μM. Docking studies revealed that this hit compound retained the necessary interactions within the active site of SARS-CoV-2 Mpro. Conclusion The identified lead natural compound could provide a scaffold for the further development of SARS-CoV-2 Mpro inhibitors.
Anaesthesia for caesarean section in a morbidly obese parturient with history of lignocaine allergy: A case report
Authors: Chia Pamela , Cheng Shang-Ming , Tan Sze-Chin , Sng Ban Leong
Published: December 2022

Morbidly obese obstetric patients present multiple challenges to the anaesthetist for labour analgesia and anaesthesia for caesarean section. The superiority of regional anaesthesia (RA) using local anaesthetics (LA) to all other techniques in the obese parturient, as well as avoiding complications of general anaesthesia (GA) in a caesarean delivery makes LA allergy in parturients an important issue. We report the first known case of lignocaine allergy in a morbidly obese parturient.
Neutrophil–lymphocyte ratio as an inflammatory biomarker of diabetic nephropathy among type 2 diabetes mellitus patients: A comparative cross-sectional study
Authors: Gurmu Mesfin Zewude , Genet Solomon , Gizaw Solomon Tebeje , Feyisa Teka Obsa , Gnanasekaran Natesan
Published: December 2022

Objective:

This study aimed to determine the neutrophil–lymphocyte ratio (NLR) as an inflammatory biomarker among type 2 diabetes mellitus (T2DM) patients with diabetic nephropathy (DN).

Methods:

A comparative cross-sectional study design was conducted on 199 T2DM patients attending Bole 17 Health Center, Addis Ababa, Ethiopia. The urine albumin test was done by the MICRAL-II test strip. Fasting blood sugar was measured by a glucometer. Complete blood count was analyzed using an automated hematology analyzer (HUMAN GmbH, Wiesbaden, Hesse, Germany). The student’s t-test, a chi-square test, and Pearson correlation were applied to analyze the data.

Results:

Out of the 199 diabetes mellitus patients, 45 (22.6%) and 154 (77.4%) were found with DN and without DN, respectively. Interestingly, the mean NLR value (2.66 ± 0.49) was found significantly higher in diabetic patients with DN compared to the mean NLR (1.65 ± 0.20) in diabetes patients without DN ( p < 0.0001). The NLR showed positive significant correlation with variables such as age ( r = 0.162, p = 0.023), duration of disease ( r = 0.52, p < 0.0001), absolute neutrophil count ( r = 0.712, p < 0.0001), total white blood cell count ( r = 0.162, p = 0.022), systolic blood pressure ( r = 0.338, p < 0.0001), and diastolic blood pressure ( r = 0.731, p < 0.0001). On the other hand, negatively significant correlation was found between NLR and absolute lymphocyte count ( r = −0.770, p < 0.000).

Conclusion:

The NLR was significantly increased in T2DM patients with DN, suggesting that inflammation and endothelial dysfunction could be an integral part of the pathogenesis of DN, and therefore, this ratio may be considered as a predictor and a prognostic biomarker of DN.
Critical stenosis of left main coronary artery as a late presentation after transcatheter aortic valve replacement: A case report and review literature
Authors: Alsaddah Jadan , Alenezi Abdullah , Maadarani Ossama , Bitar Zouheir , Alfadhli Rahaf
Published: December 2022

Summary

Transcatheter aortic valve replacement (TAVR) is an alternative treatment for selected patients with severe aortic valve stenosis who are at high risk for conventional surgery. Critical stenosis of left main coronary artery as a late complication after TAVR is quite rare.
The new era in the continuum of China and Iraq's relationship
Authors: Gul Zana
Published: December 2022

The article explores the shifts in Sino–Iraqi relations since the 1950s. The article's question is: Is China and Iraq's relationship witnessing a turning point characterised by more dependency and engagement? The article explores Sino–Iraqi interdependence throughout the last six decades through primary and secondary resources, including official documents, data on trade and Chinese and Iraqi outlets. It employs Nye and Keohane's complex interdependence to evaluate Sino–Iraqi dependency. The article finds that Sino–Iraqi interdependence was non-existent between the 1950s and 1975. At the end of the 1970s, some initial elements partially emerged but did not fully materialise; these faded in the 1990s, and since 2003 complex interdependence has gradually emerged. The bilateral relationships pre-2003 made provision for the development of the ties. This has not been without challenges, particularly from Iraq. The study shows the role of the oil sector in Sino–Iraqi interdependence, the uncertainty and extent of China's Belt and Road Initiative in Iraq and the conflicting official and public debates around it.
Managing Alzheimer’s Dementia with Homecare in an African American Family During the COVID Pandemic
Authors: Eversley Rani , Favila Wendy , Jenkins Sharon Rae , Godwin Makula , Pedrosa Maria
Published: December 2022

In the US, over 95 million people have been infected with COVID and over 1 million have died. 10% of Californians are infected with COVID with higher rates reported among Latinx, Pacific Islanders, and low-income people. Higher death rates have been reported among African Americans. People living with Alzheimer’s Disease (AD) are also more likely to be infected with COVID. African Americans with AD have three times the COVID rate of Whites. Homecare workers who care for moderate to severe AD in home and community settings are frontline essential workers who manage complex AD-related problems like incontinence. Little is known about communication and problem-solving processes between homecare workers and families of people with AD to manage continence at home. This report describes the challenges facing homecare workers illustrated by an African American family caring for a relative with advanced AD during pandemic.
Correlation Between Skin Autofluorescence and Muscle Activities of Lower Limb in Aging Without Disease and Disability
Authors: Kaneko Kenichi , Makabe Hitoshi
Published: December 2022

Skin autofluorescence is a useful index to estimate the accumulation of advanced glycation end-products in human tissues. Elderly persons with higher skin autofluorescence have lower muscle mass, muscle strength and muscle power, however, little is known about the relationship between the skin autofluorescence level and each muscle activity. We measured the values of skin autofluorescence from five places on a lower limb, and the signals of surface electromyogram during isometric contractions from five muscles on that, simultaneously. The waveforms of surface electromyogram were analyzed by Daubechies-4 wavelet transformation. The value of skin autofluorescence was increased in the proximal part of the lower limb compared with the value of the distal part. The principal component of surface electromyogram activity in a time-frequency domain was lower in the proximal part compared with that of the distal part. There was a weak negative correlation between the value of skin autofluorescence on the gluteal region and the value of the mean wavelet coefficient of the surface electromyogram signals within the gluteus maximus muscle. The higher accumulation of advanced glycation end-products on the gluteal region might suggest the lower muscle activity in aging without disease and disability.
Enhancing the Occurrence Variance Reporting System Implementation at one of the Hospitals of the Egyptian Universal Health Insurance
Authors: El-Sayed Aly Rasha Ibrahim , Ahmed Mohamed ELBahlawan Gehan , Aly Maha Ramadan , Mohamed Elsayed Boshra Karem
Published: December 2022

Background

Identifying, reporting, measuring, and tracking events provide an opportunity to study system issues, motivate learning, measure the frequency and severity of events, and manage high-risk ones which refer to a safety culture that is focused on valuing the input of working staff and improving the quality of care.

Aim

Enhance the implementation of the occurrence variance reporting (OVR) system at the Obstetrics and Gynecological Hospital in Port Said Governorate, Egypt.

Design

A quasi-experimental research design for one group (pre–posttest) and a mixed-methods approach was conducted in this study.

Method

This study was carried out at an Obstetrics and Gynecological Hospital in Port Said Governorate, Egypt. Study subjects included a convenient sample of 100 doctors and nurses. The study used three tools: OVR Knowledge, Attitude, and Practice (KAP) questionnaire, the OVR trend analysis clinical audit checklist, and barriers that hinder staff to report patient safety events through two open-ended questions.

Results

Significant improvements were detected in the OVR system post-program implementation than pre-program implementation phase. A statistically significant increase in nurses’ and doctors’ total knowledge score from 0.74 to 3.39 and a statistically significant decrease in nurses’ and doctors’ total negative attitude score from 3.87 to 3.27. Also, a statistically significant increase in total practice score from 2.35 to 2.45.

Conclusion

There were significant improvements in the hospital OVR system postprogram implementation than preprogram implementation.

Relevance to clinical practice

To maintain performance and make sure that the original result is not lost, the health care facilities should emphasize the ongoing monthly and quarterly monitoring and analysis of data. Meetings, lectures, and training sessions are used for ongoing education.
Mouth opening in systemic sclerosis: Its course over time, determinants and impact on mouth handicap
Authors: Khidir Sarah J H , Boonstra Maaike , Bergstra Sytske Anne , Boerrigter Gerry W M , Voogt-van der Harst Elles M , Ninaber Maarten K , Ajmone Marsan Nina , Huizinga Tom W J , van der Helm-van Mil Annette H M , de Vries-Bouwstra Jeska K
Published: December 2022

Objective:

Decreased maximal mouth opening is a common and disabling manifestation in systemic sclerosis patients. We aimed to study the course of maximal mouth opening, determinants of smaller maximal mouth opening over time and the burden of smaller maximal mouth opening on mouth handicap.

Methods:

Consecutive systemic sclerosis patients participating in the prospective Leiden Combined Care in systemic sclerosis cohort were included. Annual clinical assessment included maximal mouth opening measurement and mouth handicap evaluation (Mouth Handicap in Systemic Sclerosis scale). Presence of microstomia (maximal mouth opening < 30 mm) was studied. Maximal mouth opening over time was assessed on group level and for all patients individually. Baseline characteristics were analysed for their association with smaller maximal mouth opening over time (linear mixed-effects models). Furthermore, cross-sectional association between maximal mouth opening with Mouth Handicap in Systemic Sclerosis scale was assessed (linear regression analysis).

Results:

A total of 382 systemic sclerosis patients were studied with median follow-up time of 2.0 years (interquartile range = 0.0–3.0). At baseline, mean maximal mouth opening was 42.2 ± 8.0 mm and 7% suffered from microstomia. Annual decrease of > 5.0 mm in maximal mouth opening during follow-up occurred in 63 patients and was accompanied by increase in disease severity. Disease characteristics at baseline independently predictive for smaller maximal mouth opening over time were: more extended skin subtype; peripheral vasculopathy; pulmonary, renal and gastrointestinal involvement. Smaller maximal mouth opening was significantly associated with more reported mouth handicap.

Conclusion:

The course of maximal mouth opening is stable in a majority of systemic sclerosis patients. Still, maximal mouth opening over time was smaller in patients with more severe organ involvement. Although microstomia was infrequent, a smaller maximal mouth opening was significantly associated with more mouth handicap, indicating the importance to address maximal mouth opening in routine care of systemic sclerosis patients.
Chennai flyways: birds, biodiversity, and ecological decay
Authors: Gandy Matthew
Published: December 2022

The city of Chennai in southern India lies directly under one of the most significant global flyways for migratory birds. Over the last forty years, however, this intricate regional topography of lakes and watercourses that supports millions of birds and other organisms has been drastically reduced. I develop the idea of “ecological decay” in relation to the Pallikaranai wetlands in southern Chennai to explore the multiple socio-ecological dynamics behind declining levels of biodiversity. I note how the colonial simplification of the landscape has been entrained within an accelerated impetus towards regional capitalist urbanization. Thus far, however, the question of biodiversity in Chennai has been largely framed through the analytical lens of European modernity. I consider in particular whether a modified urban political ecology framework might provide distinctive insights into the particularities of biodiversity decline in an Indian context.
Understanding the linkage between ecosystem service values and ecological risk in the Yongding River basin, China
Authors: Li Jiayu , Zhou Weiqi , Qian Yuguo
Published: December 2022

Evaluations of ecosystem services values (ESV) and ecological risk (ER) are crucially important for sustainable ecosystem management and ecological security. While numerous studies have been conducted for ecosystem service values and ecological risk assessment, respectively, few studies have investigated their linkage and correlation, especially from a social-ecological perspective. Additionally, the correlation is inconsistent, varying among studies conducted at different spatial scales, which makes it difficult to apply to a building regional ecological security pattern. Here, taking the Yongding River basin, China as an example, we first quantified the spatiotemporal variation of ecosystem service values and ecological risk from 2000 to 2020 using the evaluation models of ecosystem service and ecological risk. We then explored the spatiotemporal correlation between ecosystem service values and ecological risk by using bivariate spatial autocorrelation model to identify areas where prioritized ecosystem management is required in the process of urbanization and ecological restoration. We found: (1) the total ecosystem service value in the basin decreased first, but then continued to increase. The ecosystem service value in 2020 was 679.27 million yuan lower than that in 2000. The ecosystem service values had large spatial variations, with higher ecosystem service values in mountainous areas along the distribution of the river systems. (2) Ecological risk also varied in space, with most areas having low and medium risk levels in 2020, resulting from an overall continued decrease of the ecological risk, and a shift from medium, high, and extremely high ecological risk to low and extremely low in general. (3) There was a negative spatial correlation between ecosystem service value and ecological risk, showing widely distributed “Low ecological risk vs High ecosystem service value” in non-urbanized areas, and “High ecological risk vs Low ecosystem service value” located in newly constructed land in the southeast corner of the watershed dominated. This is a result of combined effects of urban expansion and ecological restoration. Our findings can provide insights on regulation of ecological risk in the Yongding River basin, help build a regional ecological security pattern, and achieve sustainable development.
Separation of cough and reflex bronchoconstriction by inhaled local anaesthetics
Authors: Choudry NB , Fuller RW , Anderson N , Karlsson JA
Published: December 2022

Cough and airway constriction are common features of respiratory diseases. Both can be caused by stimulation of airway nerves. We have studied the effects of airway anaesthesia on these reflexes, stimulated by inhaled capsaicin, in order to determine whether they are controlled by the same sensory nerves. Ten volunteers had capsaicin cough dose responses performed before and at 10 min after inhaling placebo (ascorbic acid in saline), and the topical anaesthetics lignocaine 40 mg, and dyclonine 8 and 4 mg. The effect of the drugs on respiratory resistance (Rrs), measured using a forced oscillation technique, was measured both before and after the inhalation of a dose of capsaicin which caused less than two coughs. Lignocaine (40 mg) and dyclonine (8 mg) caused significant reports of oral anaesthesia but only lignocaine reduced the cough response to inhaled capsaicin, increasing the log dose of capsaicin causing three or more coughs by 162%. None of the treatments altered basal Rrs or its increase after inhaled capsaicin. Thus, the cough and reflex bronchoconstriction caused by inhaled capsaicin have different sensitivities to inhaled local anaesthesia, suggesting that the effect may be mediated by different sensory pathways.
Effects of exposure to welding fume: an experimental study in sheep
Authors: Naslund PE , Andreasson S , Bergstrom R , Smith L , Risberg B
Published: December 2022

Welding fume contains various metals and pulmonary effects from their inhalation are largely unknown. We have studied the effects of exposure to welding fume in sheep. The animals were exposed to either a bolus dose of welding fume solution or to five weeks daily exposure. Lung physiology parameters were studied and biopsies taken. Magnetopneumography was used to register the longterm exposure. Acutely exposed animals had elevated pulmonary arterial pressure. Arterial oxygen tension was reduced after fume instillation. These animals had accumulation of iron (Fe), magnesium (Mg) and manganese (Mn) in the lungs. Mn was elevated 40 times. Longterm exposed sheep increased the iron oxide accumulation significantly in lungs as seen with the magnetopneumographic technique. Following long term exposure, Mn was the metal most heavily retained in the lungs. Metals like Mn, Fe and Mg retained in the lungs can possibly give negative health effects. Besides this, the metals could be used for quantitation of welding fume exposure.
Airway responsiveness following wheezy bronchitis in infants
Authors: Gutkowski P
Published: December 2022

The study was undertaken to assess the airway function and its response to carbachol and salbutamol in infants recovering from wheezy bronchitis. In 82 children aged 3-33 mths, free from wheeze at the time of testing, and in 14 healthy infants, airway resistance (Raw) and thoracic gas volume (TGV) were measured using a body plethysmograph. Specific airway resistance (sRaw = Raw x TGV) was calculated. Increasing doses of nebulized carbachol were applied to challenge the airways. After a positive reaction had been achieved, 0.1 mg of nebulized salbutamol was administered. Raw was monitored during the whole procedure. In 23 of the 82 children the study was repeated after nine months on average. Within this period Raw remained elevated, whereas TGV and sRaw fell considerably (TGV from 37.9 to 28.2 ml.kg-1, p less than 0.01; sRaw from 0.78 to 0.63 kPa.s-1, p less than 0.01). Airway responsiveness also dropped during the observation period (mean log provocation dose producing 50% fall (PD50) 0.026 and 0.358, p less than 0.01). In comparison with controls the study infants responded to lower doses of carbachol (mean log PD50 0.610 and 0.031, respectively, p less than 0.01). Airway responsiveness was not related to baseline airway calibre or to signs of atopy. sRaw returned to baseline 2-5 min following salbutamol. The results suggest that airways of children in a symptom-free period following wheezy bronchitis have reduced patency and reveal hyperresponsiveness to carbachol.
Devices for low flow O2 administration
Authors: Stewart AG , Howard P
Published: December 2022

Long-term oxygen therapy requires a practical regulated oxygen delivery system which works in the home. Although face-masks are cheap and efficient, they are awkward and need removal for talking and eating. Few patients would comply with this for 15 h her day. Nasal prongs are reliable, cheap, safe and well tolerated but are wasteful of oxygen. This waste is important both practically and financially when oxygen is supplied from portable or large cylinders or from a liquid source. The Oxymizer pendants and moustache or inspiration phased delivery devices improve nasal prong efficiency. Nasopharyngeal catheters are an alternative but more invasive means of oxygen conservation. Percutaneous transtracheal catheterization is both oxygen efficient and cosmetically acceptable. As it requires an invasive surgical procedure with some serious potential complications its use should probably be reserved for those patients in whom adequate oxygenation is not achieved by standard nasal prongs. Its use is rarely justified on merely cosmetic grounds. Permanent tracheostomy is not indicated in most cases of hypoxic chronic obstructive airways disease (COAD). Nasal prongs would seem the most reasonable method of oxygen delivery. Their use with oxygen concentrators lessens the need for oxygen conservation.
Bilateral pleural effusion due to malignant mesothelioma, diagnosed by means of immunostaining
Authors: Mannes GP , Gouw AS , Postmus PE
Published: December 2022

We report a patient who presented himself with a bilateral pleural effusion. Histology proved that this was caused by a malignant pleural mesothelioma. Immunostaining and DNA-flow cytometry confirmed the diagnosis. The usefulness of these rather new diagnostic techniques is discussed.
Ventilatory responses to chemosensory stimuli in quadriplegic subjects
Authors: Pokorski M , Morikawa T , Takaishi S , Masuda A , Ahn B , Honda Y
Published: December 2022

We tested the hypothesis that interruption of motor traffic running down the spinal cord to respiratory muscle motoneurons suppresses the ventilatory response to increased chemical drive. We compared the hypoxic (HVR) and hypercapnic (HCVR) ventilatory responses, based on the rebreathing technique, before and during inspiratory flow-resistive loading in 17 quadriplegic patients with low cervical spinal cord transection and in 17 normal subjects. The ventilatory response was evaluated from minute ventilation (VE) and mouth occlusion pressure (P0.2) slopes on arterial oxygen saturation (SaO2) or on end-tidal PCO2 (PACO2), and from absolute VE values at SaO2 80% or at PACO2 55 mmHg. We found no difference in the unloaded HVR or HCVR between the quadriplegic and normal subjects. In the loaded HVR, the delta VE/delta SaO2 slope tended to decrease similarly in both groups of subjects. The delta P0.2/delta SaO2 slope was shifted upwards in normal subjects, yielding a significantly higher P0.2 at a given SaO2. In contrast, this rise in the P0.2 level during loaded HVR was absent in quadriplegics. Loaded HCVR yielded qualitatively similar results in both groups of subjects; delta VE/delta PACO2 decreased and delta P0.2/delta PACO2 increased significantly. The results show that the ventilatory chemosensory responses were unsuppressed in quadriplegics, although they displayed a disturbance in load-compensation, as reflected by occlusion pressure, in hypoxia. We conclude that the descending drive to respiratory muscle motoneurons is not germane to the operation of the chemosensory reflexes.
Influences of inhaled tobacco smoke on the senescence accelerated mouse (SAM)
Authors: Uejima Y , Fukuchi Y , Nagase T , Matsuse T , Yamaoka M , Tabata R , Orimo H
Published: December 2022

We studied the influences of inhaled tobacco smoke on lung structure, biochemical changes in bronchoalveolar lavage (BAL) fluid, and glutathione (GSH) content of the lung in the senescence accelerated mouse (SAM), using 30 female SAM-P/8 as the "senescence-prone series", compared with SAM-R/1 as the "senescence-resistant series". At 18 wks of age, half of each series were housed in Hamburg II machines and exposed to an atmosphere of tobacco smoke for 5 wks, 10 min a day, 5 days a wk. At 24 wks of age, all of the animals were sacrificed. Blood, lung, liver, kidney and eyes were removed and the contents of GSH and thiol group (-SH) were measured (n = 5). We also performed BAL, to determine its total protein, albumin, and fibronectin contents, and elastase-like activity, elastase inhibitory capacity (EIC), and trypsin inhibitory capacity (TIC) (n = 5). Histological changes of the lungs from non-lavaged animals were also examined by light microscopy (n = 5). In SAM-P/8 not exposed to tobacco smoke, the mean linear intercept was longer than that in SAM-R/1. The exposure of SAM-P/8 to tobacco smoke caused increases in its lung weight and the ratio of albumin to total protein in BAL fluid, a decrease in the EIC/TIC ratio in BAL fluid, and a decrease in the GSH content and the GSH/-SH ratio of the lung, compared with those not exposed. We also observed focal infiltration of macrophages into alveoli with hyaline membrane and thickened alveolar wall in SAM-P/8 with tobacco exposure.(ABSTRACT TRUNCATED AT 250 WORDS)
Particle deposition and resistance in the noses of adults and children
Authors: Becquemin MH , Swift DL , Bouchikhi A , Roy M , Teillac A
Published: December 2022

Nasal filter efficiency for particles has been described by several authors as showing large individual variations, probably somehow related to airflow resistance. Twelve children, aged 5.5-11.5 yrs and 8 aged 12-15 yrs were compared to a group of ten adults. Deposition of polystyrene beads (1, 2.05, 2.8 microns mass median aerodynamic diameter (MMAD] was measured by comparing inhaled aerosols and exhaled air concentrations, for both nose and mouth breathing. Ventilation was controlled to scale breathing patterns appropriate for each age either at rest or during moderate exercise to allow comparison between subjects in similar physiological conditions. Anterior nasal resistance (as a function of flow rate) and standard lung function were measured for each subject. For the same inhalation flow rate of 0.300 l.s-1, children had much higher nasal resistances than the adults, 0.425 +/- 0.208 kPa.l.1.s under 12 yrs, 0.243 +/- 0.080 kPa.l.1.s over 12 yrs and 0.145 +/- 0.047 kPa.l.1.s in adults. Individually, nasal deposition increased with particle size, ventilation flow rate and nasal resistance, from rest to exercise. The average nasal deposition percentages were lower in children than in adults, in similar conditions: at rest, 12.9 and 11.7 versus 15.6 for 1 microns; 13.3 and 15.9 versus 21.6 for 2.05 microns; 11 and 17.7 versus 20 for 2.8 microns. This was even more significant during exercise, 17.8 and 15.9 versus 29.2 for 1 microns; 21.3 and 18.4 versus 34.7 for 2.05 microns; 16 and 16.1 versus 36.8 for 2.8 microns.(ABSTRACT TRUNCATED AT 250 WORDS)
Changes in chronic respiratory symptoms in two populations of adults studied longitudinally over 13 years
Authors: Krzyzanowski M , Lebowitz MD
Published: December 2022

Data from two longitudinal studies conducted in Cracow, Poland, and Tucson, Arizona, USA, were used to evaluate the differences in period prevalence, incidence and remission rates of respiratory symptoms between two populations, as well as to assess the between-cities similarities in the relationships of the symptoms to age and smoking habit. The analysis was based on data from 3,082 adult Cracow residents, interviewed twice 13 yrs apart, and from 1,452 Tucson adults, with mean period between initial and final survey of 12.2 yrs. Log-linear models were used to consider possible interactions of the symptoms, age, smoking, gender and city. The relationship of the symptoms to smoking was similar in both cities, after adjustment for age and gender, with at least doubled incidence rates of most symptoms in continuous smokers compared to lifetime nonsmokers. The between-population differences in the symptoms were related to age, indicating onset of bronchitic symptoms occurring earlier in life in Cracow, and of asthmatic symptoms in Tucson. These differences were due to factors other than tobacco smoking e.g. various types of ambient air pollution.
Endocytosis of cigarette-smoke condensate by rabbit alveolar macrophages in vitro measured as fluorescence intensity
Authors: Skold CM , Eklund A , Hed J , Hernbrand R
Published: December 2022

It has previously been shown that alveolar macrophages (AMs) from smokers have increased fluorescence compared to AMs from nonsmokers. In the present investigation, AMs lavaged from rabbits were exposed to a cigarette-smoke condensate (CSC) in order to study its influence on the fluorescence of AMs. Flow cytofluorometry was used. Alveolar cells exposed to 0.01% of CSC showed a fluorescence which increased with higher concentrations of the CSC. Incubation with CSC for 5 min was enough to give a significant rise (p less than 0.001) in cell fluorescence compared to controls. After incubating fluorescent cells with untreated non-fluorescent AMs, fluorescent material was distributed to the non-fluorescent AMs within 30 min. Further more, fluorescence decreased when exposed cells were cultured in CSC-free medium. When incubating CSC with blood leucocytes, an almost threefold increase in fluorescence was observed among the phagocytic cells at 37 degrees C compared to 4 degrees C, whereas the fluorescence in the non-phagocytic cell population only showed a minor enhancement. Endocytosis of fluorescent material in CSC can explain the strong fluorescence seen in AMs from smokers. Exocytosis and re-endocytosis of the material by new AMs may explain the slow decline in the fluorescence of AMs observed after cessation of smoking.
Therapeutic compliance in asthma; its magnitude and implications
Authors: Cochrane GM
Published: December 2022

Failure to heed medical advice is common, but the precise extent is poorly understood and barely investigated. Factors leading to poor compliance are not known but claims of lack of education are frequently mentioned. However these have yet to be scientifically substantiated. The frequency of medication and social and economic factors, allied to poor communication, are probably involved but do not account for it entirely as non-compliance may vary between 20-80% of patient populations. Asthma mortality is not decreasing despite effective medication and increasingly there are worries that poor compliance may be a significant factor.
Identification of fish species through tRNA-based primer design
Authors: Wu Ting-Hui , Yang Cing-Han , Pai Tun-Wen , Ho Li-Ping , Wu Jen-Leih , Chou Hsin-Yiu
Published: December 2022

Background

The correct establishment of the barcode classification system for fish can facilitate biotaxonomists to distinguish fish species, and it can help the government to verify the authenticity of the ingredients of fish products or identify unknown fish related samples. The Cytochrome c oxidation I (COI) gene sequence in the mitochondria of each species possesses unique characteristics, which has been widely used as barcodes in identifying species in recent years. Instead of using COI gene sequences for primer design, flanking tRNA segments of COI genes from 2618 complete fish mitochondrial genomes were analyzed to discover suitable primers for fish classification at taxonomic family level. The minimal number of primer sets is designed to effectively distinguish various clustered groups of fish species for identification applications. Sequence alignment analysis and cross tRNA segment comparisons were applied to check and ensure the primers for each cluster group are exclusive.

Results

Two approaches were applied to improve primer design and re-cluster fish species. The results have shown that exclusive primers for 2618 fish species were successfully discovered through in silico analysis. In addition, we applied sequence alignment analysis to confirm that each pair of primers can successfully identify all collected fish species at the taxonomic family levels.

Conclusions

This study provided a practical strategy to discover unique primers for each fishery species and a comprehensive list of exclusive primers for extracting COI barcode sequences of all known fishery species. Various applications of verification of fish products or identification of unknown fish species could be effectively achieved.
RSAP-Net: joint optic disc and cup segmentation with a residual spatial attention path module and MSRCR-PT pre-processing algorithm
Authors: Jiang Yun , Ma Zeqi , Wu Chao , Zhang Zequn , Yan Wei
Published: December 2022

Background

Glaucoma can cause irreversible blindness to people’s eyesight. Since there are no symptoms in its early stage, it is particularly important to accurately segment the optic disc (OD) and optic cup (OC) from fundus medical images for the screening and prevention of glaucoma. In recent years, the mainstream method of OD and OC segmentation is convolution neural network (CNN). However, most existing CNN methods segment OD and OC separately and ignore the a priori information that OC is always contained inside the OD region, which makes the segmentation accuracy of most methods not high enough.

Methods

This paper proposes a new encoder–decoder segmentation structure, called RSAP-Net, for joint segmentation of OD and OC. We first designed an efficient U-shaped segmentation network as the backbone. Considering the spatial overlap relationship between OD and OC, a new Residual spatial attention path is proposed to connect the encoder–decoder to retain more characteristic information. In order to further improve the segmentation performance, a pre-processing method called MSRCR-PT (Multi-Scale Retinex Colour Recovery and Polar Transformation) has been devised. It incorporates a multi-scale Retinex colour recovery algorithm and a polar coordinate transformation, which can help RSAP-Net to produce more refined boundaries of the optic disc and the optic cup.

Results

The experimental results show that our method achieves excellent segmentation performance on the Drishti-GS1 standard dataset. In the OD and OC segmentation effects, the F1 scores are 0.9752 and 0.9012, respectively. The BLE are 6.33 pixels and 11.97 pixels, respectively.

Conclusions

This paper presents a new framework for the joint segmentation of optic discs and optic cups, called RSAP-Net. The framework mainly consists of a U-shaped segmentation skeleton and a residual space attention path module. The design of a pre-processing method called MSRCR-PT for the OD/OC segmentation task can improve segmentation performance. The method was evaluated on the publicly available Drishti-GS1 standard dataset and proved to be effective.
Identification of multiplicatively acting modulatory mutational signatures in cancer
Authors: Kičiatovas Dovydas , Guo Qingli , Kailas Miika , Pesonen Henri , Corander Jukka , Kaski Samuel , Pitkänen Esa , Mustonen Ville
Published: December 2022

Background

A deep understanding of carcinogenesis at the DNA level underpins many advances in cancer prevention and treatment. Mutational signatures provide a breakthrough conceptualisation, as well as an analysis framework, that can be used to build such understanding. They capture somatic mutation patterns and at best identify their causes. Most studies in this context have focused on an inherently additive analysis, e.g. by non-negative matrix factorization, where the mutations within a cancer sample are explained by a linear combination of independent mutational signatures. However, other recent studies show that the mutational signatures exhibit non-additive interactions.

Results

We carefully analysed such additive model fits from the PCAWG study cataloguing mutational signatures as well as their activities across thousands of cancers. Our analysis identified systematic and non-random structure of residuals that is left unexplained by the additive model. We used hierarchical clustering to identify cancer subsets with similar residual profiles to show that both systematic mutation count overestimation and underestimation take place. We propose an extension to the additive mutational signature model—multiplicatively acting modulatory processes—and develop a maximum-likelihood framework to identify such modulatory mutational signatures. The augmented model is expressive enough to almost fully remove the observed systematic residual patterns.

Conclusion

We suggest the modulatory processes biologically relate to sample specific DNA repair propensities with cancer or tissue type specific profiles. Overall, our results identify an interesting direction where to expand signature analysis.