bluuebunny/crossref_metadata_2025 huggingface dataset:
A Wireless Ad Hoc Network Communication Platform and Data Transmission Strategies for Multi-Bus Instruments
Authors: Qian Lushuai , Gu Kexin , Fu Yaqiong , Shen Yuli , Xu Suan
Published: September 2024

As automatic test technology advances, the number of programmable instruments in a single test system increases. Traditional wired communication methods have a limited range and involve complex cable layouts. Single-function wireless converters provide a viable alternative, but they have limitations. These include complicated configuration, issues with multi-system collaboration, and data blocking. This paper proposes a wireless ad hoc network platform for multi-bus instruments based on a low-cost ESP-12H WiFi module. The platform supports GPIB, RS232, RS485, and CAN bus interface instrument access. It features easy configuration, ad hoc networking, and self-repairing capabilities. A relay multi-hop network with a tree topology expands capacity and coverage. Additionally, a dynamic window-receiving mode and an improved multi-priority queue ensure data transmission integrity. The experimental results show that the platform’s networking time is less than 10 s, and the coverage range reaches 50 m in complex indoor environments. It also shows good stability when running for a long time. However, due to hardware and software design limitations, the actual upload speeds fall short of the theoretical values. For example, RS232 and RS485 are about 10% slower than the theoretical values, and GPIB is about 80% slower. Further optimization is required in the future.
Multi-Objective Parameter Configuration Optimization of Hydrogen Fuel Cell Hybrid Power System for Locomotives
Authors: Liu Suyao , Xu Chunmei , Zhang Yifei , Pei Haoying , Dong Kan , Yang Ning , Ma Yingtao
Published: September 2024

Conventional methods of parameterizing fuel cell hybrid power systems (FCHPS) often rely on engineering experience, which leads to problems such as increased economic costs and excessive weight of the system. These shortcomings limit the performance of FCHPS in real-world applications. To address these issues, this paper proposes a novel method for optimizing the parameter configuration of FCHPS. First, the power and energy requirements of the vehicle are determined through traction calculations, and a real-time energy management strategy is used to ensure efficient power distribution. On this basis, a multi-objective parameter configuration optimization model is developed, which comprehensively considers economic cost and system weight, and uses a particle swarm optimization (PSO) algorithm to determine the optimal configuration of each power source. The optimization results show that the system economic cost is reduced by 8.76% and 18.05% and the weight is reduced by 11.47% and 9.13%, respectively, compared with the initial configuration. These results verify the effectiveness of the proposed optimization strategy and demonstrate its potential to improve the overall performance of the FCHPS.
Blockchain-Based Spectrum Sharing Algorithm for UAV-Assisted Relay System
Authors: Huang Fukang , Zhu Qi
Published: September 2024

Unmanned aerial vehicles (UAVs) are promising tools in mobile communication due to their flexibility. However, the rapid development of mobile communications further intensifies the challenge of spectrum scarcity, necessitating spectrum sharing with other systems. We suggest a Spectrum Sharing Algorithm for a UAV-Assisted Relay System. The utility function of secondary users (SUs) is defined by their communication rate, rewards from relay primary users (PUs), and spectrum leasing expenses. The utility function of PUs consists of their communication rate and revenue from spectrum leasing. We propose a joint optimization algorithm for the positioning and power allocation of UAVs, maximizing the frequency spectrum utilization rate of users. Spectrum trading between PUs and SUs is modeled as a Stackelberg game, and the problem is solved by using Lagrange multipliers and KKT conditions. To enhance the security of spectrum trading, a reputation-based spectrum sharing blockchain consensus algorithm is designed. We utilize Shamir’s secret sharing method to reduce computational complexity. Additionally, we design a smart contract to optimize the functionality of transaction transfers. The findings demonstrate that the proposed algorithm enhances the benefits for both participants in spectrum sharing, while safeguarding the security of spectrum transactions.
Development of Solid-State Lithium-Ion Batteries (LIBs) to Increase Ionic Conductivity through Interactions between Solid Electrolytes and Anode and Cathode Electrodes
Authors: Monajjemi Majid , Mollaamin Fatemeh
Published: September 2024

Although in general ions are not able to migrate in the solid-state position due to rigid skeletal structure, in some solid electrolytes with a low energy barrier and high ionic conductivities, these ion transition can occur. In this work, we considered several solid electrolytes including lithium phosphorus oxy-nitride (LIPON), a lithium super-ionic conductor (SILICON), and thio-LISICON. For the fabrication and characterization of the solid electrolyte’s fabrication, we used a single-step ball milling (SSBM) procedure. Through this research on all-solid-state rechargeable lithium-ion batteries, our target is to discuss solving several problems in solid LIBs that have recently escalated due to raised concerns relating to safety hazards such as solvent leakage and the flammability of the liquid electrolytes used for commercial LIBs. Through this research, we tested the conductivity amounts of various substrates containing amorphous glass, SSBM, and glass-ceramic samples. Obviously, the SSBM glass-ceramics increased the conductivity, and we also found that the values for conductivity attained by SSBM were higher than those values for glass-ceramics. Using an SSBM technique, silicon nanoparticles were used as an anode material and it was found that the charge and discharge curves in the battery cell cycled between 0.009 and 1.45 V versus Li+/Li at a current density of 210 mA g−1 at room temperature. Since high resistance causes degradation between the cathode material (LiCoO2) and the solid electrolyte, we added GeS2 and SiS2 to the Li2S-P2S5 system to obtain higher conductivities and better stability of the electrode–electrolyte interface.
On-Board Chargers for Electric Vehicles: A Comprehensive Performance and Efficiency Review
Authors: Dar Abrar Rasool , Haque Ahteshamul , Khan Mohammed Ali , Kurukuru Varaha Satya Bharath , Mehfuz Shabana
Published: September 2024

The transportation industry is experiencing a switch towards electrification. Availability of electric vehicle (EV) charging infrastructure is very critical for broader acceptance of EVs. The increasing use of OBCs, due to their cost-effectiveness and ease of installation, necessitates addressing key challenges. These include achieving high efficiency and power density to overcome space limitations and reduce charging times. Additionally, the growing interest in bidirectional power flow, allowing EVs to supply power back to the grid, highlights the importance of innovative OBC solutions. This review article provides a thorough analysis of the current advancements, challenges, and prospects in EV on-board charger technology. It aims to offer a comprehensive review of OBC architectures, components, technologies, and emerging trends, guiding future research and development. Addressing these challenges is essential to enhance the efficiency, reliability, and integration of OBCs within the broader EV ecosystem.
Semi-Empirical Model Based on the Influence of Turbulence Intensity on the Wake of Vertical Axis Turbines
Authors: Wang Ziyao , Hou Erhu , Wu He
Published: September 2024

In the context of energy shortages and the development of new energy sources, tidal current energy has emerged as a promising alternative. It is typically harnessed by deploying arrays of multiple water turbines offshore. Vertical axis water turbines (VAWTs), as key units in these arrays, have wake effects that influence array spacing and energy efficiency. However, existing studies on wake velocity distribution models for VAWTs are limited in number, accuracy, and consideration of influencing factors. A precise theoretical model (Lam’s formula) for wake lateral velocity can better predict wake decay, aiding in the optimization of tidal current energy array designs. Turbulence in the ocean, serving as a medium for energy exchange between high-energy and low-energy water flows, significantly impacts the wake recovery of water turbines. To simplify the problem, this study uses software ANSYS Fluent 2020 R2 for two-dimensional simulations of VAWT wake decay under different turbulence intensities, confirming the critical role of turbulence intensity in wake velocity decay. Based on the obtained data, a new mathematical approach was employed to incorporate turbulence intensity into Lam’s wake formula for VAWTs, improving its predictive accuracy with a minimum error of 1%, and refining some parameter calculations. The results show that this model effectively reflects the impact of turbulence on VAWT wake recovery and can be used to predict wake decay under various turbulence conditions, providing a theoretical basis for VAWT design, optimization, and array layout.
The Determination of Woody Biomass Resources and Their Energy Potential from Hazelnut Tree Cultivation
Authors: Borkowska Anna , Maj Grzegorz , Klimek Kamila E. , Kapłan Magdalena
Published: September 2024

The aim of this study was to estimate the shoot weight of four selected hazelnut cultivars and to see if the morphological characteristics of the cultivar and the age of the shoots affect their quality when used as fuel. This study shows that the cultivar ‘Olga’ generated the highest amounts of woody biomass (6507 t·ha−1), while ‘Olbrzymi z Halle’ generated the lowest (3843 t·ha−1). ‘Olbrzymi z Halle’ had the highest calorific values (HHVs) (18.08 MJ·t·ha−1 for annual shoots and 18.03 MJ·kg−1 for perennial shoots) and ‘Olga’ had the lowest calorific values (16.64 MJ·kg−1 for annual shoots and 16.39 MJ·kg−1 for perennial shoots). The age of the shoots had a minimal effect on the chemical and energy parameters. Emissions were the highest for ‘Olbrzymi z Halle’ (CO: 57.74 MJ·kg−1 for perennial shoots, CO2: 1414.05 MJ·kg−1) and lowest for ‘Olga’ (CO: 50.57 MJ·kg−1, CO2: 1238.46 MJ·kg−1). The cultivar ‘Olbrzymi z Halle’, which generated the least amount of biomass compared to the other cultivars, stands out for its high energy value due to its low moisture and ash contents and its high carbon and hydrogen contents, making it attractive for the purposes of biofuel production and supporting sustainable agriculture. The practical implications of the research findings include the selection of suitable varieties for biofuel production, the management of biomass moisture content, and the optimisation of combustion techniques to reduce emissions. The potential for using hazelnut shoots as a biofuel highlights the importance of sustainable agriculture and renewable energy production. The results provide valuable information that can support decisions regarding the cultivation and use of hazelnut shoots for biofuel production while minimising negative environmental impacts.
Optimal Scheduling Strategy for Urban Distribution Grid Resilience Enhancement Considering Renewable-to-Ammonia Coordination
Authors: Jiang Li , Hu Fei , Zong Shaolei , Yan Hui , Kong Wei , Chai Xiaoguang , Zhang Lu
Published: September 2024

The integration of numerous distributed energy sources into the power system offers exciting opportunities to enhance the resilience of distribution networks. It is worth noting that the renewable-to-ammonia system has the potential to alleviate the multi-temporal and spatial imbalance of the power system. Therefore, this paper proposes a mathematical model for a renewable-to-ammonia system, taking into account the material balance and power balance of each unit. Based on this, this paper further explores the optimization scheduling method for flexible ammonia loads in distribution networks. A relaxation method for branch flow models in distribution networks based on second-order cone programming is proposed. An optimization scheduling model for flexible ammonia loads in distribution networks is constructed to minimize network loss. Moreover, considering the environmental advantages of the renewable-to-ammonia system, this paper compares the changes in hydrogen production technologies under different carbon emission constraints. Finally, a case study of the IEEE 33-node system is adopted to verify the effectiveness of the proposed model and method. It indicates that the renewable-to-ammonia system has environmental benefits and can reduce network loss to a certain extent.
Integrated Transcriptome and Metabolome Analyses Provided Insight into the Variation in Quality of Green Bamboo (Bambusa oldhamii) Shoots at Different Growth Stages
Authors: He Yuelin , Cao Xiangmei , Pan Kaiting , Shi Wenhui , Ying Yeqing
Published: September 2024

Green bamboo (Bambusa oldhamii) shoots are not only delicious but also highly nutritious. However, their palatability and quality changed significantly upon being unearthed, making them unsuitable for commercial sale and subsequently diminishing their market value. To clarify the mechanisms that regulate the quality of green bamboo shoots at different growth stages, we conducted a comprehensive analysis of the metabolome and transcriptome at the unearthed height of 0 cm (H0), 5 cm (H5), and 10 cm (H10). Metabolome analysis identified 149 differentially accumulated metabolites (DAMs) among H0, H5, and H10, primarily comprising phenolic acids, lipids and flavonoids. Metabolic pathways enriched by these DAMs included phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, linoleic acid metabolism and alpha-linolenic acid metabolism. Further transcriptome analysis identified 2976 differentially expressed genes (DEGs) among H0, H5, and H10. Additionally, KEGG analysis indicated that these DEGs mainly enriched pathways associated with metabolic pathways, biosynthesis of secondary metabolites, and phenylalanine metabolism. We screened out 10 DEGs and 16 intermediate metabolites of these pathways. Furthermore, we identified six DEGs with expression patterns highly correlated with the content of lignin and the total flavonoids from H0 to H5 and H10. Finally, RT-qPCR analysis of six genes validated the transcriptome sequencing data. Our findings indicate significant quality variations in green bamboo shoots from H0 to H5 and H10. These variations are closely related to key genes involved in the synthesis of lignin and flavonoids, which result in the flavor and quality changes of green bamboo shoots from the belowground stage to unearthed stage.
Characterizing Roughness of Wooden Mortise and Tenon Considering Effects of Measured Position and Assembly Condition
Authors: Hu Wen-Gang , Yu Run-Zhong , Yang Peng
Published: September 2024

The surface roughness of wood mortise and tenon seriously influence the strength of mortise-and-tenon joints. However, it is difficult to obtain the whole surface roughness of mortise and tenon due to the limitation in measuring range of most profilometers. Therefore, the selection of measured position is critical to measuring the roughness of mortise and tenon. This study mainly aimed to investigate the effects of measured position in the thickness direction (T1, T2, and T3) and length direction (L1, L2, and L3), and assembly condition (unassembled and assembled) on the surface roughness of wood mortise and tenon to characterize their surface roughness. Roughness profile results showed that the average roughness of mortise (4.53 µm) was greater than that of tenon (3.89 µm), and the roughness of unassembled ones was greater than that of assembled ones. The roughness at all measured positions of unassembled mortise was nearly identical, while for unassembled tenon, roughness in the thickness direction varied. T2 was significantly greater than those of T1 and T3. Statistical analysis results showed that for an unassembled sample, sample type and measured position in the thickness direction significantly influenced roughness, but measured position in the length direction was not significant. Assembly condition significantly influenced the roughness of tenon; the roughness of assembled tenon decreased significantly compared with unassembled tenon. The roughness at T2L1 decreased so much compared with T2L2 and T2L3. It can be concluded that the roughness of mortise was mainly dominated by its grain orientation of the measured surface, which was perpendicular to the grain. The roughness of unassembled tenon varied and resulted from the tangential feeding speed of the machine changing during the manufacturing of the curved part of the tenon. The cutting speed at T2 of wood tenon was faster than those of T1 and T3, so the roughness at T2 was greater than those of T1 and T3.
Effects of Growth and Treatment Conditions on the Quality of Norway Spruce (Picea abies L.) Sawn Timber
Authors: Krenn Tobias , Berthold Dirk , Ritter Nina , Kietz Bettina
Published: September 2024

A study was conducted to improve the effectiveness of silvicultural production of structural sawn timber from softwoods. It intends to explore prediction methods for mechanical timber quality. The study material was obtained from six stands divided into age groups of approximately 40- and 80-year-old trees (examining the influence of age). The stands were differentiated by their applied thinning system of thinning from below or above (examining the influence of the thinning system). Resulting from these different levels of data, i.e., stand parameters, tree anatomy, and visual board properties are examined and analyzed in ordinal logistic models and linear mixed models. Visual board properties were discerned by means of the German standard for visual grading of sawn timber. The mechanical board properties were measured in on-edge bending strength tests and allocated into strength classes, which were modeled in dependence of visual characteristics and forestry conditions. The evaluation of mechanical properties attributed a significant loss of timber quality to short rotation periods, non-ideal water supply, and a single-tree management system. The prediction capabilities of models based on site and tree characteristics were on par with the accuracy of visual grading. Management adaptations by intense thinning from above can lead to a significant decline in Norway spruce (Picea abies L.) timber quality when site factors coincide. Particular care should be taken in the management of locations with high yield potential. Non-destructive evaluation based on site characteristics combined with terrestrial laser scan data of tree characteristics has potential as a pregrading method.
The Impact of Forestry Technological Innovation on the Welfare of Farm Households Managing Jujube Forests (Ziziphus jujuba Mill.) in the Lüliang Mountains of the Yellow River’s Middle Reaches
Authors: Wang Jin , Jiang Xuemei , Chen Xingliang , Zhang Jingjing , Dou Yaquan , Zhang Jing
Published: September 2024

Jujube (Ziziphus jujuba Mill.) makes up a traditional characteristic industry with ecological significance in the Lüliang Mountain of middle reaches of the Yellow River (LMMRYR). However, low economic efficiency has reduced local farm households’ willingness to continue jujube cultivation, threatening the sustainable maintenance and development of jujube forests and the ecological environment. In response, Lüliang City implemented a technological innovation program, that is, the Jujube Forest High Grafting and Optimization Program (JFHGOP), in 2018. Based on survey data from 302 local farm households, an empirical analysis using propensity score matching and ordinary least squares methods revealed that the program significantly enhanced the economic, ecological, and social benefits for participating farm households, improving their overall welfare. Robustness tests confirmed these findings, and a heterogeneity analysis showed varied impacts across different dimensions. The program improved welfare through government support and cooperatives’ assistance. To further promote green development and farm households’ welfare, recommendations include advancing forestry innovation technology, supporting small farm households with policy, capital, and technology, optimizing subsidy mechanisms, supporting new business entities, and promoting cooperation and benefit-sharing among stakeholders.
Numerical Simulation Studies on the Design of the Prosthetic Heart Valves Belly Curves
Authors: Zhou Jingyuan , Wu Yinkui , Chen Lu , Li Tao , Xiong Yan , Chen Yu
Published: September 2024

Prosthetic heart valves (PHVs) are employed to replace the diseased native valve as a treatment of severe aortic valve disease. This study aimed to evaluate the effect of curvature of the belly curve on valve performance, so as to support a better comprehension of the relationship between valve design and its performance. Five PHV models with different curvatures of the belly curve were established. Iterative implicit fluid–structure interaction simulations were carried out, analyzing in detail the effect of belly curvature on the geometric orifice area (GOA), coaptation area (CA), regurgitant fraction (RF), leaflet kinematics and stress distribution on the leaflets. Overall, GOA and CA were negatively and positively related to the curvature of the belly curve, respectively. Nevertheless, an excessive increase in curvature can lead to incomplete sealing of free edges of the valve during its closure, which resulted in a decrease in CA and an increase in regurgitation. The moderate curvature of the belly curve contributed to reducing RF and fluttering frequency. Valves with small curvature experienced a significantly higher frequency of fluttering. Furthermore, all stress concentrations intensified with the increase in the curvature of the belly curve. The valve with moderate curvature of the belly curve strikes the best compromise between valve performance parameters, leaflet kinematics and mechanical stress. Considering the different effects of the curvature of belly curve on valve performance parameters, the PHV design with variable curvature of belly curve may be a direction towards valve performance optimization.
Inhibition of Amyloid β Accumulation by Protease-Digested Whitebait (Shirasu) in a Murine Model of Alzheimer’s Disease
Authors: Katsuki Takahiro , Ogi Kayako , Kinno Ayaka , Kasamatsu Shingo , Ihara Hideshi , Sumitani Hidenobu
Published: September 2024

The number of people with dementia is increasing annually worldwide. Alzheimer’s disease (AD), which accounts for the highest percentage of dementia-causing diseases, remains difficult to cure, and prevention of its onset is important. We aimed to discover new AD-preventive ingredients and investigate the inhibitory effects of ten different species of seafood digests prepared by protease treatment on β-secretase 1 (BACE1) activity. Substantial inhibition of BACE1 activity was observed in five species of seafood, and protease-digested whitebait (WPD) showed the highest inhibitory effect among the ten marine samples. We further examined the potential of WPD as an AD preventive component using a familial AD strain (5xFAD) murine model. The intraperitoneal administration of WPD for 28 days substantially decreased the insoluble amyloid β1–42 content and the expression of glial fibrillary acidic protein, a marker of astrogliosis, in the cerebral cortex of the 5xFAD mice. These results strongly suggest that WPD is a novel functional food-derived ingredient with preventive effects against AD.
Encapsulation of Phenolic Compounds Extracted from Beet By-Products: Analysis of Physical and Chemical Properties
Authors: Guamán-Balcázar María del Cisne , Montero Magdalena , Celi Alejandro , Montes Antonio , Carrera Ceferino , Pereyra Clara , Meneses Miguel Ángel
Published: September 2024

Beet is a nutritious and health-promoting food with important bioactive compounds in its industrial by-products. The encapsulation of antioxidants from beet by-products has been proposed for valorization. For this, an ethanol–water extract was mixed with polyvinylpyrrolidone (PVP) (used as a carrier agent) and then encapsulated. The encapsulation was performed by spray drying, where the effects of temperature (140–160 °C), extract input flow rate (10–30%), and extraction solvent (ethanol–water 50/50 v/v and ethanol) were evaluated for the total phenol content and the spray-drying yield. The yields obtained were between 60 and 89%, and total phenols were between 136 and 1026 mg gallic acid equivalents/g of encapsulated product. Both responses were affected (p < 0.05) by the extraction solvent. The optimal spray-drying conditions were determined by response surface methodology (RSM). The encapsulated product obtained at optimal conditions was characterized by infrared spectrometry, X-ray fluorescence, Ultra-High Performance Liquid Chromatography, and scanning electron microscopy analysis. The results show that the encapsulated product has a high content of total phenols and compounds such as betanin, isobetanin, and neobetanin. Considering the results of physicochemical properties and the bioactive compounds, the optimized encapsulated product could be applied in the food industry as a bioactive ingredient or natural colorant. However, the further investigation of alternative carrier agents needs to be performed to reduce caking.
Characterization, Antioxidant Capacity, and Anti-Inflammatory Activity of Polyphenol-Enriched Extracts Obtained from Unripe, Mature, and Overripe Fruits of Red-Fleshed Kiwifruit Cultivars
Authors: Yang Qian-Ni , Deng Wen , Wu Ding-Tao , Li Jie , Liu Hong-Yan , Yan Hui-Ling , Du Kui , Hu Yi-Chen , Zou Liang , Huang Jing-Wei
Published: September 2024

Discarded unripe kiwifruits (DUKs) are regarded as the major agro-byproducts in the production of kiwifruits, which have abundantly valuable secondary metabolites. Nevertheless, owing to the limited knowledge about the differences in phytochemicals and bioactivity between DUKs and mature kiwifruits, the utilization of DUKs in the food industry remains scarce. Hence, to promote their food applications, the phenolic compounds and bioactivity of discarded unripe, mature, and overripe fruits from three red-fleshed kiwifruit cultivars were studied and compared. The results revealed that the levels of total phenolics, total flavonoids, and total procyanidins in kiwifruits varied significantly by maturity stage. In addition, our findings demonstrated that DUKs possessed much higher contents of valuable phenolic compounds (e.g., chlorogenic acid (CHA), neochlorogenic acid (NCHA), gallic acid (GA), protocatechuic acid (PA), procyanidin B1 (ProcB1), procyanidin B2 (ProcB2), procyanidin C1 (ProcC1), quercetin 3-O-glucoside (QueG), and quercetin 3-O-rhamnoside (QueR)) than mature and overripe kiwifruits. Furthermore, DUKs exerted much stronger in vitro antioxidant capacity, inhibitory effects on α-glucosidase, and anti-inflammatory activity than mature and overripe kiwifruits, which were mainly attributed to their higher contents of total polyphenols and individual phenolic components, such as GA, CHA, NCHA, PA, ProcB1, ProcB2, ProcC1, and QueR. Overall, these findings provide sufficient evidence for the development and utilization of DUKs in the food/functional food industry.
Impact of Particle Size on the Physicochemical, Functional, and In Vitro Digestibility Properties of Fava Bean Flour and Bread
Authors: Olakanmi Sunday J. , Jayas Digvir S. , Paliwal Jitendra , Aluko Rotimi E.
Published: September 2024

Fava beans, renowned for their nutritional value and sustainable cultivation, are pivotal in various food applications. This study examined the implications of varying the particle size on the functional, physicochemical, and in vitro digestibility properties of fava bean flour. Fava bean was milled into 0.14, 0.50, and 1.0 mm particle sizes using a Ferkar multipurpose knife mill. Physicochemical analyses showed that the 0.14 mm flour had more starch damage, but higher protein and fat contents. Functionality assessments revealed that the finer particle sizes had better foaming properties, swelling power, and gelation behavior than the coarse particle size. Emulsion capacity showed that for all the pH conditions, 1.00 mm particle size flour had a significantly higher (p < 0.05) oil droplet size, while the 0.5 and 0.14 mm flours had smaller and similar oil droplet sizes. Moreover, in vitro digestibility assays resulted in improved starch digestion (p ˂ 0.05) with the increase in flour particle size. Varying the particle size of fava bean flour had less impact on the in vitro digestibility of the bread produced from wheat–fava bean composite flour, with an average of 84%. The findings underscore the critical role of particle size in tailoring fava bean flour for specific culinary purposes and nutritional considerations.
Evaluation of the Bioavailability of Iodine and Arsenic in Raw and Cooked Saccharina japonica Based on Simulated Digestion/Caco-2 Cell Model
Authors: Li Na , Geng Zhaomeng , Guo Yingying , Dai Xinyue , Zhu Wenjia , Yao Lin , Jiang Yanhua , Wang Xiaojuan , Dong Hao , Wang Huijie , Wang Lianzhu
Published: September 2024

Kelp is a traditional healthy food due to its high nutritional content; however, its relatively high contents of iodine and arsenic have raised concerns about its edible safety. This study explored the effects of different cooking treatments on the contents of iodine and arsenic in kelp, evaluated the bioaccessibility and bioavailability of iodine and arsenic in kelp using in vitro digestion, and compared the differences in the transport characteristics of iodine in kelp and KIO3 using a Caco-2 monolayer cell transport model. The results show that the content of target elements that reached systemic circulation could be reduced by cooking and gastrointestinal digestion. The highest reductions in iodine and arsenic were 94.4% and 74.7%, respectively, which were achieved by boiling for 10 min. The bioaccessibility and bioavailability of iodine and arsenic were significantly improved by a cooking treatment. However, the contents of iodine and arsenic decreased significantly, with the bioaccessibility of iodine reducing from 3188.2 μg/L to 317.0 μg/L and that of arsenic reducing from 32.5 μg/L to 18.1 μg/L in the gastric phase after boiling. The findings also show that the efficiency of iodine transport in kelp and KIO3 was positively correlated with the transport time and negatively correlated with the concentration of iodine. With the increase in the iodine concentration, the rate of iodine transport in kelp decreased from 63.93% to 3.14%, but that of KIO3 was stable at around 35%, which indicates that the absorption efficiency of iodine from kelp was limited, even when too much kelp was ingested. In conclusion, the edible safety of kelp is significantly improved after cooking. The risk of excessive iodine and arsenic intake caused by consuming kelp is extremely low, and as an effective iodine supplement source, kelp has higher edible safety compared with KIO3. This study clarifies the safety of algae based on iodine and arsenic contents and also provides a basis for the formulation of food safety standards.
Analysis of Aroma Characteristics of ‘Binzi’ and ‘Xiangguo’ Apple—Ancient Cultivars in China
Authors: Lu Xiang , Liu Zhao , Gao Yuan , Wang Kun , Sun Simiao , Guo Hanxin , Tian Wen , Wang Lin , Li Zichen , Li Lianwen , Feng Jianrong , Wang Dajiang
Published: September 2024

‘Binzi’ (BZ) (Malus domestica subsp. chinensis var. binzi Li Y.N.) and ‘Xiangguo’ (XG) (Malus domestica subsp. chinensis var. xiangguo Li Y.N.) are the ancient cultivars in China. The BZ fruits have a low-fragrant flavor on harvest day but a high-fragrant flavor after storage at room temperature, while the XG fruits have a stronger flavor when mature. ‘Starking’ (SK) and ‘Golden Delicious’ (GD) fruits have a rich flavor and are recognized by all countries in the world. However, information on the differences between ancient Chinese cultivars and Western apple cultivars in aroma compounds remains unknown. The apple fruits were collected for continuous two years. Aroma compounds in the skin and pulp of the fruits were detected at room temperature (20 ± 1 °C) during storage. The dynamics of VOCs in BZ and SK fruits were more similarly reflected in esters, while those of XG and GD fruits were reflected in aldehydes and alcohols. Ethyl 2-methylbutyrate, with an extremely low odor threshold, was the main source of typical apple flavor in SK, BZ, and XG fruits, while hexyl acetate was the source of the banana flavor in GD fruits. 6-methyl-5-hepten-2-one and β-damascenone were the important ketones produced in the later stage of storage, derived from the carotenoid metabolism pathway and providing a citrus and rose flavor to the four apple cultivars. SK had the highest number of characteristic aroma components, which were mainly derived from the amino acid metabolism pathway, providing fruits with a sweet and fruity flavor. Although the characteristic aroma components of GD were derived from the fatty acid metabolic pathway, the number of volatile esters was lower. Ethyl butyrate, derived from the saturated fatty acid metabolism, had the highest content in BZ, providing a pineapple flavor; the flavor of XG was mainly derived from ethyl 2-methylbutyrate, 6-methyl-5-hepten-2-one, and β-damascenone. Therefore, we suggest BZ and XG apples as the aroma-breeding material with which to enrich new cultivars’ aroma components, derived from the fatty acid metabolism and carotenoid metabolism pathways, respectively.
Investigating the Effects of Pink-Generating Ligands on Enhancing Color Stability and Pigment Properties in Pork Sausage Model Systems Cured with Sodium Nitrite or White Kimchi Powder
Authors: Bae Su Min , Jeong Jong Youn
Published: September 2024

In this study, we investigated the effects of different nitrite sources (sodium nitrite or white kimchi powder) and pink-generating ligands (cysteine, histidine, or nicotinamide) on the development and stability of cured meat color in pork sausage model systems over 30 d of refrigerated storage. The samples were prepared in a 2 × 3 factorial design with two nitrite sources and three ligands, and their physicochemical properties were evaluated on days 0, 15, and 30. Although white kimchi powder induced cured color development similar to that of synthetic sodium nitrite, it resulted in higher cooking loss and lower residual nitrite content in cured pork sausages (p < 0.05). The addition of cysteine resulted in significantly higher CIE a* values, cured meat pigment, and curing efficiency than histidine and nicotinamide (p < 0.05), while yielding lower pH values, residual nitrite content, and total pigment content (p < 0.05). The storage duration significantly reduced the residual nitrite and total pigment contents of the products. These findings suggest that white kimchi powder can serve as a natural alternative to sodium nitrite in pork sausage models and that the incorporation of cysteine has a favorable impact on the development and enhancement of cured meat color.
SVA Regulation of Transposable Element Clustered Transcription within the Major Histocompatibility Complex Genomic Class II Region of the Parkinson’s Progression Markers Initiative
Authors: Kulski Jerzy , Pfaff Abigail , Koks Sulev
Published: September 2024

SINE-VNTR-Alu (SVA) retrotransposons can regulate expression quantitative trait loci (eQTL) of coding and noncoding genes including transposable elements (TEs) distributed throughout the human genome. Previously, we reported that expressed SVAs and human leucocyte antigen (HLA) class II genotypes on chromosome 6 were associated significantly with Parkinson’s disease (PD). Here, our aim was to follow-up our previous study and evaluate the SVA associations and their regulatory effects on the transcription of TEs within the HLA class II genomic region. We reanalyzed the transcriptome data of peripheral blood cells from the Parkinson’s Progression Markers Initiative (PPMI) for 1530 subjects for TE and gene RNAs with publicly available computing packages. Four structurally polymorphic SVAs regulate the transcription of 20 distinct clusters of 235 TE loci represented by LINES (37%), SINES (28%), LTR/ERVs (23%), and ancient transposon DNA elements (12%) that are located in close proximity to HLA genes. The transcribed TEs were mostly short length, with an average size of 389 nucleotides. The numbers, types and profiles of positive and negative regulation of TE transcription varied markedly between the four regulatory SVAs. The expressed SVA and TE RNAs in blood cells appear to be enhancer-like elements that are coordinated differentially in the regulation of HLA class II genes. Future work on the mechanisms underlying their regulation and potential impact is essential for elucidating their roles in normal cellular processes and disease pathogenesis.
SeqSNP-Based Targeted GBS Provides Insight into the Genetic Relationships among Global Collections of Brassica rapa ssp. oleifera (Turnip Rape)
Authors: Geleta Mulatu , Sundaramoorthy Jagadeesh , Carlsson Anders S.
Published: September 2024

Turnip rape is a multi-purpose crop cultivated in temperate regions. Due to its ability to fit into crop rotation systems and its role as a food and feed source, spring-type turnip rape cultivation is on the rise. To improve the crop’s productivity and nutritional value, it is essential to understand its genetic diversity. In this study, 188 spring-type accessions were genotyped using SeqSNP, a targeted genotyping-by-sequencing method to determine genetic relationships between various groups and assess the potential effects of mutations within genes regulating major desirable traits. Single nucleotide polymorphism (SNP) alleles at six loci were predicted to have high effects on their corresponding genes’ functions, whereas nine loci had country/region-specific alleles. A neighbor-joining cluster analysis revealed three major clusters (I to III). About 72% of cluster-I accessions were of Asian origin, whereas 88.5% of European accessions and all North American accessions were placed in cluster-II or cluster-III. A principal coordinate analysis explained 65.3% of the total genetic variation. An analysis of molecular variance revealed significant differentiation among different groups of accessions. Compared to Asian cultivars, European and North American cultivars share more genetic similarities. Hence, crossbreeding Asian and European cultivars may result in improved cultivars due to desirable allele recombination. Compared to landraces and wild populations, the cultivars had more genetic variation, indicating that breeding had not caused genetic erosion. There were no significant differences between Swedish turnip rape cultivars and the NordGen collection. Hence, crossbreeding with genetically distinct cultivars could enhance the gene pool’s genetic diversity and facilitate superior cultivar development.
Limb Perfusion Delivery of a rAAV1 Alpha-1 Antitrypsin Vector in Non-Human Primates Is Safe but Insufficient for Therapy
Authors: Pires-Ferreira Debora , Reil Darcy , Tang Qiushi , Blackwood Meghan , Gallagher Thomas , Keeler Allison M. , Chichester Jessica A. , Vyhnal Kristin K. , Lindborg Jane A. , Benson Janet , Fu Dongtao , Flotte Terence R. , Gruntman Alisha M.
Published: September 2024

Background/Objectives: α-1 antitrypsin (AAT) deficiency is an inherited, genetic condition characterized by reduced serum levels of AAT and increased risk of developing emphysema and liver disease. AAT is normally synthesized primarily in the liver, but muscle-targeting with a recombinant adeno-associated virus (rAAV) vector for α-1 antitrypsin (AAT) gene therapy has been used to minimize liver exposure to the virus and hepatotoxicity. Clinical trials of direct intramuscular (IM) administration of rAAV1-hAAT have demonstrated its overall safety and transgene expression for 5 years. However, the failure to reach the therapeutic target level after 100 large-volume (1.5 mL) IM injections of maximally concentrated vector led us to pursue a muscle-targeting approach using isolated limb perfusion. This targets the rAAV to a greater muscle mass and allows for a higher total volume (and thereby a higher dose) than is tolerable by multiple direct IM injections. Limb perfusion has been shown to be feasible in non-human primates using the rAAV1 serotype and a ubiquitous promoter expressing an epitope-tagged AAT matched to the host species. Methods: In this study, we performed a biodistribution and preclinical safety study in non-human primates with a clinical candidate rAAV1-human AAT (hAAT) vector at doses ranging from 3.0 × 1012 to 1.3 × 1013 vg/kg, bracketing those used in our clinical trials. Results: We found that limb perfusion delivery of rAAV1-hAAT was safe and showed a biodistribution pattern similar to previous studies. However, serum levels of AAT obtained with high-dose limb perfusion still reached only ~50% of the target serum levels. Conclusions: Our results suggest that clinically effective AAT gene therapy may ultimately require delivery at doses between 3.5 × 1013–1 × 1014 vg/kg, which is within the dose range used for approved rAAV gene therapies. Muscle-targeting strategies could be incorporated when delivering systemic administration of high-dose rAAV gene therapies to increase transduction of muscle tissues and reduce the burden on the liver, especially in diseases that can present with hepatotoxicity such as AAT deficiency.
Transient Myeloproliferative Disorder (TMD), Acute Lymphoblastic Leukemia (ALL), and Juvenile Myelomonocytic Leukemia (JMML) in a Child with Noonan Syndrome: Sequential Occurrence, Single Center Experience, and Review of the Literature
Authors: Arrabito Marta , Li Volsi Nicolò , La Rosa Manuela , Samperi Piera , Pulvirenti Giulio , Cannata Emanuela , Russo Giovanna , Di Cataldo Andrea , Lo Nigro Luca
Published: September 2024

Noonan syndrome (NS) is an autosomal dominant disorder that varies in severity and can involve multiple organ systems. In approximately 50% of cases, it is caused by missense mutations in the PTPN11 gene (12q24.13). NS is associated with a higher risk of cancer occurrence, specifically hematological disorders. Here, we report a case of a child who was diagnosed at birth with a transient myeloproliferative disorder (TMD). After two years, the child developed hyperdiploid B-cell precursor acute lymphoblastic leukemia (BCP-ALL), receiving a two-year course of treatment. During her continuous complete remission (CCR), a heterozygous germline mutation in the PTPN11 gene [c.218 C>T (p.Thr73lle)] was identified. At the age of ten, the child presented with massive splenomegaly, hyperleukocytosis, and thrombocytopenia, resulting in the diagnosis of juvenile myelomonocytic leukemia (JMML). After an initial response to antimetabolite therapy (6-mercaptopurine), she underwent haploidentical hematopoietic stem cell transplantation (HSCT) and is currently in complete remission. The goal of this review is to gain insight into the various hematological diseases associated with NS, starting from our unique case.
An Observational Study on the Management of Medico-Legal Maxillofacial Trauma Cases by General Practitioners
Authors: Giuvara Constantin Răzvan , Costan Victor Vlad , Boisteanu Otilia , Armencia Adina , Ciofu Mihai , Cernei Eduard Radu , Balcos Carina , Iliescu Bulgaru , Calin Gabriela , Hurjui Loredana Liliana
Published: September 2024

Maxillofacial trauma, as seen from a medico-legal point of view, is an integral part of medical practice in emergency departments. Therefore, general practitioners should have sufficient knowledge about their roles and responsibilities in managing these cases. This study aimed to assess general practitioners’ knowledge, practices, and attitudes regarding managing medico-legal cases (MLCs). Material and method: This study included 113 general practitioners from St. Spiridon Hospital in Iasi, Romania. Participants completed a self-administered structured questionnaire assessing the knowledge, attitudes, and practices of general practitioners regarding the handling of medico-legal cases. Results: The scores obtained for the level of knowledge, attitudes, and practices indicate a good level of knowledge on the part of the responding doctors, with the average value being 38, in a range from 0 to 49 (min. value 28–max wave. 47). The score regarding the attitudes of doctors related to the management of medico-legal cases is modest, with the average value being 37 points out of a maximum of 60 points (min. 14–max. 51). The same situation is recorded in the case of practices regarding the management of medico-legal cases, with the average value being 68 out of a maximum value of 90 (min. 38–max. 84). Conclusion: This study’s results revealed the absence of a well-defined protocol for the recognition and handling of medico-legal cases among general practitioners from Iasi and the need to improve the level of attitudes and practices regarding the management of medico-legal cases. The limitations of this study included the relatively small sample from a single hospital and the use of a methodology based on self-administered questionnaires, which may be subjective. Accordingly, future studies should involve larger and more diverse samples to monitor changes in knowledge and practices over time and qualitative methodologies to gain deeper physician-related insights into medical case management.
Temporal Mortality Trends Attributable to Stroke in South Asia: An Age–Period–Cohort Analysis
Authors: Bai Ruhai , Li Minmin , Bhurtyal Ashok , Zhu Wenxuan , Dong Wanyue , Dong Di , Sun Jing , Su Yanfang , Li Yan
Published: September 2024

South Asia contributes the most to stroke mortality worldwide. This study aimed to determine the long-term trends in stroke mortality across four South Asian countries and its associations with age, period, and birth cohort. In 2019, nearly one million stroke deaths occurred across South Asia, and the associated age-standardized mortality rate (ASMR) was 80.2 per 100,000. Between 1990 and 2019, India had the largest decrease in the ASMR (−35.8%) across the four South Asian countries. While Pakistan had the smallest decrease in the ASMR (−7.6%), an increase was detected among males aged 15 to 34 years and females aged 15 to 19 years. Despite a 22.8% decrease in the ASMR, Bangladesh had the highest ASMR across the four South Asian countries. Nepal reported a witness increase in the stroke ASMR after 2006. Improved period and cohort effects on stroke mortality were generally indicated across the analyzed countries, except for recent-period effects in males from Nepal and cohort effects from those born after the 1970s in Pakistan. Stroke mortality has decreased in the four South Asian countries over the past 30 years, but potentially unfavorable period and cohort effects have emerged in males in Nepal and both sexes in Pakistan. Governmental and societal efforts are needed to maintain decreasing trends in stroke mortality.