bluuebunny/crossref_metadata_2025 huggingface dataset:
Inventory model of goods availability with apriori algorithm
Authors: Husna Radhiatul , Lestari Riri , Hendra Yomei
Published: November 2019

In this study, mathematical modeling will be discussed to determine the probabilityof goods purchase based on other goods. Research on this topic is considered to be important and interesting to study because it describes the implementation of applied mathematics by constructing a mathematical model and solving it using an apriori algorithm related to the application of the probability concept. In this case, the apriori algorithm is used to determine the pattern of dependency relationships between goods and other goods so that the probabilityof goods purchase based on other goods can be found. To get the output of this research which is in the form of the mathematical modeling for determining the probability of goods being purchased depended on the purchase of other goods, then selecting and classifying the types of goods based on the sales, using the apriori algorithm, constructing models, finding a solution of models and interpreting of the model are conducted.
Optimal reinsurance based on compound Poisson distribution
Authors: Chadidjah Anna , Noviyanti Lienda , Zanbar Soleh Achmad
Published: November 2019

BPJS Kesehatan (BPJS-Health) is a public agency for health, established to implement the social security program. The health insurance prepared through BPJS is in the form health protection for participants to get health care benefits and protection to meet the basic needs of health care. It is provided to every person who has paid fees or whose contributions are paid by the government. Claims estimation in BPJS-Health is required to increase the optimal service to participants. Imprecision in determining such estimates could destabilize the BPJS-Health. In this research, estimated total health service claims submitted by partnered health facility to BPJS based on Buhlmann-Straub Credibility. We also use the compound Poisson distribution to illustrate the optimal retentions in a collective risk model.
Optimal reinsurance using the expected shortfall
Authors: Noviyanti Lienda , Zanbar Soleh Achmad , Chadidjah Anna
Published: November 2019

The Financial Services Authority Regulation regarding Self-Retention and Domestic Reinsurance Support (Otoritas Jasa Keuangan / OJK Regulation) was issued to establish a more comprehensive framework concerning self-retention and reinsurance support of insurance companies. Insurance Companies must implement and maintain a self-retention limit based on their risk and loss profile. This requires Insurance Companies to make available a certain amount of capital for every risk that they undertake. Insurance companies must develop and implement a reinsurance support strategy to ensure that they have sufficient capacity to meet their liabilities. In this research, we estimate the optimal retention by means of Expected Shortfall which will minimize the risk of individual total based on the quota share reinsurance. We use lognormal bivariate distribution to illustrate the optimal retention in an individual risk model.
Synthesis of silver nanoparticles used chemical reduction method by glucose as reducing agent
Authors: Eka Putri Gusliani , Rahayu Gusti Feni , Novita Sary Annisa , Zainul Rahadian
Published: November 2019

Silver nanoparticles have been successfully synthesized using a chemical reduction method at room temperature using glucose as a reducing agent, silver nitrate as a source of nitrate, polyvinyl alcohol (PVA) as a stabilizing agent. The purpose of this research was to determine the effect of the mole ratio of reducing agent Glu / Ag and percentage (%) PVA (b/v) in the silver nanoparticle synthesis of the resulted product. Product characterization was analyzed by UV-Vis Spectrophotometers, XRD, and TEM. The Analysis of the UV-Vis spectra showed that the most stable particles were silver nanoparticles used PVA 3% and Glu / Ag. 1: 8 on λmax 415 to 417 nm. TEM’s characterization showed that silver nanoparticles were spherical with a dominant size range of 12,28 nm – 38,45 nm nm. The newly synthesized silver nanoparticles may have a potential for antibacterial applications.
Preparation of dye sensitized solar cell (DSSC) using isolated anthocyanin fromfruit sat (melastomamalabathricum l) dicopimented with salicylic acid as dye
Authors: Hardeli  , Indra A , Rahadian
Published: November 2019

A study has been conducted on the use of anthocyanin extract from fruits (Melastomamalabthricum L), which was incubated by the addition of salicylic acid as photosensitizer in DSSC solar cells. The objective of this study was to determine the optimum condition of the extracts of copigmenation which resulted in the optimum efficiency of DSSC solar cells by increasing the absorption ability of anthocyanin pigment photons.The method used is the method of copigmentation. Copigmentation is an anthocyanin reaction with a copigment compound to form a more complex anthocyanin structure with a number of double bonds that can absorb more photons than the original anthocyanin.. The results of dye characterization using UV-Vis and FTIR indicate a wider absorption spectrum and a more complex structure in antigianated anthocyanins when compared without copigmentation. Based on the results of the characterization of the prepared TiO2 thin film, obtained crystal size of TiO2 used ranged from 27.377 to 64.738 nm with a width of200-950 nm photocathode spekrum absorption. Based on the results of the research, it is found that the antiganant extract can be increased by DSSC solar cell efficiency from 0.62% to 1.32% with 24 hours of copigmentation time and 1: 2 concentration of concentration.
Preparation and characterization of thin film CoFe2O4/Zn/CoFe2O4 by using spin-coating method
Authors: Sanjaya Hary , Oktavia Budhi , Lasdeni Sadri Lira , Ramli
Published: November 2019

Research has been conducted on the manufacture of CoFe2O4 / Zn/CoFe2O4 thin films using sol gel-spin coating method. This study aims to look at the effect of rotational speed on optical properties and electrical properties, and to find the highest GMR ratio. Making CoFe2O4 uses Co2+ precursors and Fe2+ precursors with a mole ratio of 1: 2, dissolved with ethyl alcohol. The Zn precursor was made with a concentration of 0.5 M from Powder (CH3COO)2Zn. 2H2O dissolved with ethyl alcohol. The rotation speed variations used are at 2000 rpm, 2500 rpm, 3000 rpm, 3500 rpm and 4000 rpm. The thin layer of GMR is made with a sandwich structure. CoFe2O4/Zn/CoFe2O4 thin films with variations in rotational speed were tested for electrical properties using the 4-point probe system method and characterized using X-Ray Diffraction, Scanning Electron Microscopy (SEM), and UV-Vis Specrophotometer. Based on the testing of the electrical properties obtained the lowest resistance and resistivity values at 2000 rpm rotation speed, where the faster the rotational speed is used, the greater the resistance and resistivity values. The highest GMR ratio obtained was 53.5%. Measurement results with Scanning Electron Microscopy (SEM) obtained layer thickness of 3.5 μm.
Amino acid and mineral composition of moringa oleivera leaves extract and its bioactivity as antioxidant
Authors: Natsir H , Wahab A W , Budi P , Dali S , Arif A R
Published: November 2019

Moringa leaf (Moringaoleifera) is a plant that grows in Indonesia with high nutritional content. This plant has biological activity as antidiabetic, blood pressure-lowering, and antioxidant. This study analyzes the nutritional content of Moringa leaf extract, especially amino acids, minerals and determines its antioxidant. Analysis of total amino acid content has been carried out by HPLC (High performance liquid chromatography) method, minerals with ICP-OES (Inductive coupled plasma-optical emission spectroscopy) and antioxidant activity by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The analysis of Moringa leaf extract has obtained 15 kinds of amino acids, namely: threonine (9403.09), lysine (11694.16), leucine (18087.41), isoleucine (9321.59), phenylalanine (17236.01), valine (11183.48), methionine (5684.68), tryptophan (2577.82) while non-essential amino acids include histidine (9965.39), proline (10068.07), tyrosine (8641.63), aspartate acid (16585.76), glycine (13027.13), arginine (13123.94), alanine (14474.52), glutamate acid (30106.87), serine (10055.98), cysteine (470.37) in ppm. The analysis of minerals has obtained 13 essential minerals included Al, Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Ni, P, Se, Zn. The highest mineral is shown for selenium (1097.84 ppm) and chrome (919.99 ppm) at the second highest order. Sodium (68.83 ppm) and magnesium (60.84 ppm) is found as minerals with low concentrations. The antioxidant test has shown weakness activity with an average IC50 value of 9.901 μg/mL, using ascorbic acid as a positive control with IC50 values of 196.892 μg/mL. The analysis results of amino acids, minerals, and antioxidant activity show that Moringa leaves are very potential for human nutrients.
Synthesis and characterisation of a partially methylated dodecyl thiomaltotrioside derivative as a precursor of cyclodextrin analogue
Authors: Parbuntari H , Sakairi N , Purwono B , Swasono R T
Published: November 2019

Due to the enermous roles of cyclodextrin combining with small organic molecules, more recent interests study about the manipulation of cyclodextrin structure giving better performance for any kinds of field but they presented long synthetis steps as producing the precursors. This study points out the synthesis of precursor of β-cyclodextrin analouges with maltotriose as raw materials giving the short synthesis steps. The product structures were analysed by Fourier Transform Infrared (FTIR) Specstroscopy,and characterised by1H-,13C- NMR, COSY NMR, and ESI Mass Spectroscopy. The result showed thatO-acetylation reaction produced maltotriose derivative 1 (95%), thioglycosidation reaction produced maltotriose derivative 2 (54%), de-acetylation reaction produced maltotriose derivative 3 (97%),O-benzylidation reaction produced a mixture of maltotriose derivatives 4,O- methylation reaction produced maltotriose derivative 5 (46%) and de-benzylidation reaction produced maltotriose derivative 6 (85%).
Distribution and sources of polycyclic aromatic hydrocarbon (PAH) on sediment around makassar coast
Authors: Syahrir Muhammad
Published: November 2019

This study conducts an analysis related to distribution and source of PAH in sediment around Makassar coast. Sediment sample has been taken up using Van Veen grab and put in a plastic sac, brought to laboratory, added with salt, stored in dried freezer, and followed for PAH analysis. Sample preparation has been carried out using DCM and n-hexane (1:1) solvent to obtain extract of organic matter (EOM). The EOM is fractionated to yield alkane fraction (F1) and aromatic fraction (F2) containing PAH. The GC-FID has been used to determine PAH concentration through analysis of F2. Validation of the analytical method applies linearity, LoD, and LoQ. The recovery test has fulfilled for all PAH compounds except for naphthalene and fluoranthene. The highest concentration of PAHs is found at station 7 with benzo(a)antracene 933.23±3.88 ppm and perylene 205.75±19.45 ppm. The lowest total concentration of PAHs is found at station 12 located at the estuary of Tello River caused by dilution from both river and sea water. The source of PAHs in sediment is generally from petrogenic and pirolytic process.
Synthesis hydroxyapatite/collagen/chitosan composite for tissue engineering
Authors: Cahyaningrum S E , Herdyastuti N , Puspita F W , Devina B , Kurniasari M
Published: November 2019

Hydroxyapatie/Collagen/Chitosan composite has been synthesized by ex-situ methods. The purpose of this research is to study the composition’s effect of the composite HA/Coll/Chi to some characters such as a functional group and phase as well as the physical character such as crystallinity, the compressive strength and surface morphology. HA synthesized from the precursor calcium derived from duck eggshell reacted with H3PO4, HA sintered at 900 °C. HA was added with collagen and chitosan solutin with a mass ratio of the composition HA/Coll/Chi are 7: 2: 1; 7: 1,5: 1,5; 7: 1: 2. FTIR analysis showed a band PO4
3−and OH− adsorption of HA, as well as a shift vibration of spectra in the group C = O and NH2 from chitosan and collagen that shows there has been a bond between collagen, chitosan and HA. Data of XRD showed that the phase of HA and chitosan in the composite of HA/Coll/Chi. Composite with collagen component hight has low cristalinity.
Immunostimulant activity of steroid compound from the indonesian silver fern (P. calomelanos)
Authors: Sutoyo S , Ismono  , Mitarlis  , Hidajati N , Rinaningsih
Published: November 2019

A secondary metabolite in steroid compound, namely β-sitosterol had been isolated from the n-hexane extract of the Indonesian silver fern. Extraction was conducted by maceration method, separation by chromatography techniques (vacuum liquid chromatography and flash chromatography), purification by recrystallization, and identification of its molecular structure by the spectroscopic methods and by comparison with reported literature data. The steroid isolate was yielded as colorless amorphous powder (melting point of 136– 137 °C). Based on the carbon clearance method, isolated steroid showed immunostimulant activity with phagocytosis index of 1.625.
Characterization Membrane Composition Of PVA-Enzyme Coating PVC-KTpClPB As Urea Sensor With UV-VIS, SEM-EDX and XRD
Authors: Abd Hakim S , Tarigan Krista , Situmorang Manihar , Sembiring Timbangen
Published: November 2019

Potentiometrie ion sensor (ISE) is very attractive because of its small size, easy to carry, more durable, and miniature easy to use. ISE consists of an indicator electrode and a reference electrode located in an electrolyte solution. Potentiometric electrodes and electrochemical properties can be achieved through membrane synthesis and selectivity by the interaction of primary ions with respect to cavity size, molecular geometry and types of functional groups leading to the interaction between electrolyte solutions, membranes and indicator electrodes with reference electrodes. Indicator electrodes function as urea analytes. Potentiometric characteristics are significantly dependent on the composition of the membrane, the nature of the plasticizers. Characterization of membrane composition was carried out using (UV-Vis, SEM-EDX, and XRD). Characterization of PVA membrane : PVC in the composition of 1 : 1 of 0.0350 g PVA and plasticizer 0.0700 g KTpClPB variation of membrane electrode layer and enzyme 1 mg. The optimum amount of diffraction intensity is obtained from PVA E 4X PVC-KTpClPB 0.0700.
Satellite-based monitoring of forest cover change in indonesia using google earth engine from 2000 to 2016
Authors: Fadli A H , Kosugo A , Ichii K , Ramli R
Published: November 2019

The Google Earth Engine (GEE) is a cloud computing system providing data analysis platform with various satellite based observation data and other earth observation datasets. Therefore, application of the GEE enable us to analyze terrestrial environemtal changes in an effective way. This study aims to test GEE capability to monitor forest cover change in Indonesia from 2000 to 2016 by performing raster and vector manipulations using Landsat-based forest cover datasets. Deforestation is one of the most important environmental problem in Indonesia, and monitoring of forest cover changes is urgent task for environmental protection. The found that GEE is very effective to analyze large amount of remote sensing data quickly, and estimated forest cover area in 2000 and its interannual variations in Indonesia. Factors affecting land cover in Indonesia will be elaborated in further detail in this paper.
Application of plastic optical fiber material as pH measurement sensor using loop configuration
Authors: Arifin A , Hardianti  , Yunus M , Dewang S
Published: November 2019

Plastic optical fiber testing as a sensor can be applied in pH measurement using loop configuration. It was made of a plastic optical fiber material with two types of peel, namely with cladding and without cladding. Sensor testing was done by dipping the sensor into buffer solutions with varying pH values. The light intensity from the LED will experience power losses due to the effect of adding the solution refractive index around the sensor, so the phototransistor will receive smaller the light intensity. Changes of light intensity in the optical fiber causes the output voltage read on the microcontroller and the computer decreases. The best measurement was obtained at sensor type of without cladding 4 loops with sensitivity 0.035 V/pH and resolution 0.029 pH. Aplication of plastic optical fiber as sensor for pH measurement has advantages such as low cost, real time, high sensitivity, accurate and simple measurement.
Stopping power and inelastic mean free path of 300 eV–50 keV electrons for lanthanum aluminate
Authors: Tahir Dahlang , Yulianti  , Suarga
Published: November 2019

SP (Stopping Power) and IMFP (Inelastic Mean Free Path) of lanthanum alumínate (La2O3)x (Al2O3)1–x was varied with x = 0.25, 0.34, and 0.5. for electron energy from 300 eV to 50 keV have been determined by using composite Simpson from the equation of Born Ochkur correction. The main parameter in determining the SP and IMFP is Energy Loss Function (ELF) which was obtained from the experimental reflection electron energy loss (REELS) spectra. The method in this study is convenient methods for determining SP for binary compound lanthanum aluminate.
Structure and mechanical properties of electrodeposited Ni-AlN/Si3N4 composite coating
Authors: Budi Esmar , Fathia Nurul , Indrasari Widyaningrum , Sugihartono Iwan
Published: November 2019

N1-AIN/Si3N4 composite coatings were successfully deposited on tungsten carbide by electrodeposition process. The influence of Si3N4 powder concentration on the microstructure, crystal structure and mechanical properties was investigated by using Scanning Electron Microscopy, Energy Dispersive Spectroscopy, X-Ray Diffraction and Vickers hardness tester, respectively. The results revealed that variation of Si3N4 concentration influenced the structure and mechanical properties of Ni-AlN/Si3N4 composite coatings. The fine surface morphology of composite was due to the inclusion of AlN and Si3N4 particles while it is rougher as the Si3N4 concentration was increased due to agglomeration effect and Ni crystal growth. The increase of microhardness is related to the particles dispersion- strengthening.
Quantitative analysis of X-Ray diffraction spectra for determine structural properties and deformation energy of Al, Cu and Si
Authors: Heryanto  , Abdullah B , Tahir D , Mahdalia
Published: November 2019

X-Ray Diffraction (XRD) method was used to analysis structural properties of Al, Si and Cu as cubic crystal system. Existence of Al, Si and Cu was investigated with the joint committee on powder diffraction standards (JCPDS) by 96-901-3104 for Si, 96-431-3215 for Al and 96-901-1605 for Cu respectively. In this paper we determine the concentration of our sample from the 3 or 4 peak area and the intensity of each peak. The content of Al, Si and Cu are 78,97%, 80,69% and 82,51% respectively. Relative texture coefficient (RTC) value investigated from diffraction by using h k l plane. Energy density, stress and strain were calculated using uniform density model (UDM), uniform stress deformation model (USDM), uniform deformation energy density model (UDEDM) and size-strain plot method. For the crystallite size we applied the Scherrer equation, Williamson-Hall (W-H) analysis and Size-Strain plot (SSP) method. High correlations the crystallite size found from the results by Scherrer, W-H, and SSP.
Enrichment of omega-3 fatty acids, waste oil by-products canning tuna (thunnus sp.) with urea crystallization
Authors: Wayan Suriani Ni , Komansilan Alfrits
Published: November 2019

Enrichment test of omega-3 fatty acids from tuna canning waste oil in Bitung North Sulawesi Indonesia have been conducted. The purpose of this study was to utilize tuna canning waste oil and enriched the concentration of omega-3 fatty acids with urea crystallization. The results showed that the characteristics of fish oil quality of tuna canning waste 0.9% moisture content, free fatty acids (FFA) 3.4%, copper (Cu) 0.1 ppm, iron (Fe) 2.5 ppm and phosphorus (P) 32,5 ppm still meets IFOMA quality standards Liquid fatty acid profile contains total SFA 33.7%, 27.6% PUFA, 1.4% omega-6 fatty acids, omega-3 fatty acids 25.9%, EPA 4.2% and DHA 21.4%, MUFA 18.8%, omega-9 fatty acids 13.9%, while concentrations of omega-3 fatty acids contained a total SFA of 7.7%, PUFA 70.9%, omega-6 fatty acids 0.8%, omega-3 fatty acids 70.1%, EPA 4.2% and DHA 21.4%, MUFA 2.7%, omega-9 fatty acids 1.5%. The urea crystallization process increases the levels of polyunsaturated fatty acids (PUFAs) such as omega-3 (EPA and DHA.), the enrichment rate is 2.7 times.
Effect of the indigenous trichoderma application on germination of black glutinous rice seed
Authors: Anhar Azwir , Permata Sari Novita , Advinda Linda , Hilda Putri Dwi , Handayani Dezi
Published: November 2019

Trichoderma is one of the rhizosphere microorganisms that are able to play as plant growth promoting fungus (PGPF) because it can produce IAA hormone. The ability of Trichoderma as PGPF has been extensively studied, but the indigenous Trichoderma effect derived from the rhizosphere of rice crops on local rice variety of west Sumatera has not been widely studied. This study aims to determine the effect of Trichoderma application on black glutinous rice seed germination. The experiment is done by soaking the local black sticky rice seeds with Trichoderma spore suspension with 107 spore / mL density for 24 hours. The germination rate of each isolate observed at seven days after sowing. The results showed that the type of isolate affected the rate of germination of rice seeds. The highest germination was found in isolates from the rhizosphere of rice varieties cisokan balang (94%) and the lowest was in the control (74%).
The fractions of phenolic and flavonoid compounds of the leaves of north Sulawesi’s bashful plant (mimosa pudica linn)
Authors: Rahardiyan D , Poluakan M , Moko E M , Ngangi J
Published: November 2019

The bashful plant or the mimosa plant has a history of use for treatments in various ailments. Various parts of this plant maybe used, but most commonly the leaves, stems, roots and flowers. This research was aimed to investigate the existence of phenolic and flavonoids in the Mimosa pudica Linn leaf extracts in solvents of different polarities. During this research dried leaves and wet leaves were respectively macerated in 70% ethanol and then reduced by rotary evaporators. Fractions were obtained by means of HPLC (High Pressure Liquid Chromatography) with 3 different solvents at different polarities (hexane, n-butanol and ethyl-acetate). The results indicated that flavonoid was found mostly in the ethyl-acetate fraction from dry leaves (1.08% w/w). Phenolic was mostly detected in extracts of the dry leaves in the n-butanol (7.27% w/w) and the wet leaf residues (8.68% w/w). Results indicates that with the majority of the detection of phenolics and flavonoids in the mildly polar to strong polar solvents, thus oral intake by means of leaf infusions (such as tea/brew) would be quite effective. Complete extraction process of these compounds and encapsulating them would always be the most effective method of intake while economically the method would raise questions.
Demographic profile and pap smear cytology in female sexual workers at primary health care Bestari Medan-Petisah
Authors: Anggraini D R , Feriyawati L , Wahyuni A S , Widyawati T , Sari M I , Syarifah S
Published: November 2019

Sexual workers are one of risk the cervical cancer has a long latent period. Cervical cancer is the main cancer in Southeast Asia and the second most common cancer in Indonesia. In addition, Indonesia is the second common country cause of death in women after Vietnam. The aim of this study was to evaluate demographic profile and cervical cytology in Female Sexual Workers (FWSs) at Primary Health Care Bestari, Medan-Petisah, North Sumatera. This study has recruited 70 FWSs who has worked for at least 2 years and are not pregnant. Participants fill out the questionnaires include age of participants, age at first sexual intercouse, and life styles (smoking of cigarette and consumption of alcohol). Pap smear test from cervical smear of 70 FWSs were conducted using Papanicolaou staining. The result showed most of the FWSs of sexually-active women less than 30 years old reported 54,28% and 45,72% more than 30 years old. The first sexual intercourse showed 18-20 years old as much as 54,29%, life styles of smoking and consumption alcohol was 51,4% and 10% respectively. Pap smear cytology showed 52,9% was inflammatory smear, 15,7% was A squamous Cells of Undetermined Significance (ASCUS), 11,4% was Low-grade Squamous Intra epithelial Lesions (LSIL). Most of FWSs described inflammatory smear, although a small portion had been found of ASCUS and LSIL. The chronic inflammation can develop to be carcinogenesis.
Bioinformatic analysis of truncated envelope protein in C-terminal stem-anchor region: as strategies for increasing protein secretion
Authors: Putri D H , Fiffendy M
Published: November 2019

One type of Dengue vaccine candidate that has the potential to be developed is DNA vaccine based on the DENV envelope gene. Constraints in developing vaccines are low secretion of envelope proteins. One of the efforts made to increase the secretion of envelope protein is by deletion the stem / anchor area. The purpose of this analysis was to analysis the effect of cutting the stem / anchor region on character and structure of the DENV E protein. Analysis of composition, profile, location and structure of proteins was using http://www.expasy.org, TMHMM, and http://bioinf.cs.ucl.ac.uk/psipred. Deletion of the stem-anchor region was changes the general properties of the protein, as well as the protein topology. However, the mutations did not change the 3D structure of main protein domain.
Effect of wheat grass juice (triticum aestivum l.) against the erythrocytes and hemoglobin in male mice (mus musculus l.) anemia induced by sodium nitrite
Authors: Yuniarti E , Hasanah L , Advinda L , Indika P M
Published: November 2019

Anemia is one of the most common health problems found in Indonesia. Anemia is characterized by the low number of erythrocytes and hemoglobin in the blood caused by low levels of iron in the body. Wheat grass (Triticum aestivum L.) contain high existence of iron is supposed to be able to increase erythrocytes and Hb level in mice (Mus musculus L.). Completely Randomized Design was used in this experiment, consisting of 5 treatment (K1: without NaNO2 and WGJ (wheat grass juice), K2: given the NaNO2 without given the WGJ, P1: WGJ 25%, P2: WGJ 50%, and P3: WGJ 75%) and 5 replication. The parameters observed were the male mice erythrocytes and the Hb levels before and after being given the wheat grass juice. The data were analyzed using ANOVA then continued DNMRT test with a 5% level. The results showed that giving wheat grass juice for 14 days can increased the eryhrocytes and Hb levels in male mice. The most noticeable impact is the treatment with the highest dose i.e. P3 (WGJ 75%).
The effect of the swietenia mahagoni seed extracts on the production of collagen in human fibroblast cell (HSF1184)
Authors: Hartati  , Salleh Liza Md , Che Yunus Azizi , Abd Azis Azila , Pagarra Halifah , Rachmawaty
Published: November 2019

We have used the in vitro sircol collagen test to investigate the effects of Swietenia mahagoni seed extract on collagen production in human fibroblast cells (HSF1184). The results showed that Swietenia mahagoni seed extract with concentrations of 0.001, 0.01 and 0.1 mg / mL caused stimulation in the synthesis of type I collagen in HSF1184 cells. Although, three concentration of SC-CO2 and soxhlet that showed no statistically significant of collagen on the HSF1184 when compared with the negative control, while LA (fibroblast cells with pure Linoleic acid as supplement in culture medium) gave significant effect on the collagen production.
The potential of lactic acid bacteria to improve the quality and number of carnocine during fermentation process of Bakasang as a functional food
Authors: Lawalata Helen J. , Rungkat Jovialine A.
Published: November 2019

The objectives of the research are to study the potensial of lactic acid bacteria to inhibit the growth histamine-producing bacteria, inhibit the formation of indicator component off-flavor of bakasang and increasing the amount of carnosine as a bioactive peptide. The results indicated that Pediococcus sp.B5.1 and Pediococcus sp.B1.0 were able to increase the amount of carnosine by 9.16 x and 8.92 x, able to inhibit the growth histamine-producing bacteria, soluble proteins increased by 10.4% and 10.5%, capable of inhibit the formation of histamine 5.8 mg / 100g and 5.9 mg / 100 mg, TVN (47.2 mg / 100gr and 45.6 mg / 100g), and TMA (24.2 mg / 100g and 22.6 mg / 100 g ). This shows that the two isolates of BAL are able to improve the quality and maintain the safety of fermented foodsbakasang and that most the impotantly can increase the potential of bakasang to become functional food because it can increase the number of carnosine.
Utilization and management of landscape unit in Dayak ngaju community in three villages, Mantangai sub-district, Kapuas Regency, Central Kalimantan
Authors: Puspita Sari Indri , Nisyawati  , Rohmat Sofiah
Published: November 2019

Dayak Ngaju community especially in three villages, which are Tumbang Muroi, Tumbang Mangkutub, and Katimpun in Mantangai Sub-district, Kapuas Regency, Central Kalimantan has used environment unit as a place to fulfill the community’s daily needs. The perception of the community towards each landscape unit utilization has been held in July 2018. The result of classification and utilization of the landscape unit is a belief and trust of the people which is able to influence the perception of the community towards sustainable utilization and management of the environment. Thus, this research provides explanation about utilization, development, and management based on Dayak Ngaju community norm in order to sustainably conserve the natural resources in the future.
Characterization extrinsic factors of immobilized cells thermoxilanolytic bacteria in producing xylanase
Authors: Irdawati I , Putri D D , Syamsuardi S , Agustien A , Rilda Y
Published: November 2019

Enzyme is one of important commodity in industrial world. The majority of industrial enzymes are produced by microorganisms through fermentation. The use of enzymes is largely as a biocatalyst in chemical transformations. Currently there are about 400 known enzymes and about 200 are used commercially. The enzyme industry has grown rapidly and occupies an important position in the field of industry one of which is xylanase enzyme. Xylanase can be produced by molds and bacteria through a fermentation process. Groups of bacteria that are known to produce xylanase are Bacillus and Clostridium. In today’s microbial use of thermophilic bacteria has commercial value because it is able to produce stability of heat-resistant enzymes. In producing enzymes cell immobilization techniques are used to facilitate the purification of products, increase productivity and ease in controlling cell stability. The aim of this study was to Characterization Extrinsic Factors of Immobilized Cells Thermoxilanolytic Bacteria in Producing Xylanase. This research was conducted in June 2017- June 2018 in Laboratory of Microbiology FMIPA UNP. This research was using descriptive method. This research conclusion that temperature effect on xylanase activity with optimum temperature was 70°C and pH affects the xylanase activity with the highest pH was 7.5.
Cellulase induction enzymes characteristics of hindguts of endemic termites of North Sulawesi
Authors: Ngangi Jantje , Mauren Moko Emma , Rahardiyan Dino
Published: November 2019

The necessity for enzyme utilization in various industrial applications and reducing production cost for a more economical process compelled this research to seek for an alternative source of endemic enzyme for various sources cellulotic enzymes. The termites hindgut is known as a fermentor chamber of its own containing bacterial isolates with the potential to induce cellulosic substrates. Series of research have been conducted to obtain cellulolytic potential bacterial isolates characterized biochemically and molecularly. The purpose of this research was to determine the enzyme characters of cellulolytic bacterial from the hindguts of endemic termites of North Sulawesi. Screening results from various bacterial isolates from hindguts of local termites of several regencies from North Sulawesi identified Bacillus cereus from the hindgut of Odontotermes javanicus from South Minahasa (North Sulawesi). This Bacillus cereus isolate had the best cellulolytic potential by exhibiting a cellulolytic index of 1.75 cm on a selective media of CMC, with the best enzyme production on the 6th day with the activity of 0.0054U/ml.
Cytological features of pap smear of multiparous women with mycoplasma hominis and ureaplasma urealyticum infection at outpatient clinic in medan
Authors: Feriyawati L , Nasution TA , Anggraini DR , Wahyuni AS , Widyawati T
Published: November 2019

Mycoplasma hominis and Ureaplasma urealyticum were commensal pathogenic- potential bacterias in the female urogenital tract and may cause of newborns death. Several studies have suggested a correlation between cervical cytology and Mycoplasma genitalium and Ureaplasma urealyticum infections. It was a cross sectional design carried out on 50 multiparous women in an outpatient clinic in Medan in 2018. The cervical cytology features observed were reactive cellular change (RCC), cannonballs, predominance of coccoid bacteria, clue cells, and large number of neutrophils. All cytology features were negative from 3 (6.0%) subjects that had Mh infection. 9 (18.0%) subjects with Uu infection showed negative result of RCC, 9 (100.0%) were positive cannonballs, 3 (33.3%) were predominance of coccoid bacteria, 1 (11.1%) were positive clue cells and 7 (77.8%) had large number of neutrophils. Cytology finding of 38 (76.6%) subjects with no infection of Mh and Uu revealed 10 (26.3%) had RCC, 28 (73.7%) were positive cannonballs, 19 (50.0%) were predominance of coccoid bacteria, 2 (5.3%) were positive clue cells and 16 (42.1%) had large number of neutrophils. Non-specific cytological features were found in Mycoplasma infection. Specific features found in Ureaplasma infection but need to be traced to the infection of other organisms in group non Mycoplasma and Ureaplasma infections.
The effect of hyptis suaveolens (l.) poit extract on the growth of sclerotium rolfsii with in-vitro
Authors: Chatri M. , Handayani D. , Primayani S.A
Published: November 2019

Sclerotium rolfsii is a fungus that has a wide range of host and high pathogenicity that can cause stem rot, root rot, and wilt in plants. Diseases caused by S. rolfsii can be solved by using synthetic or chemical fungicides. The use of synthetic or chemical fungicides on an ongoing basis can have a negative impact on human health. Natural pesticides derived from plant leaf extracts are explored to treat plant diseases. One of them is Hyptis suaveolens (L.) Poit. The compounds contained in this plant are alkaloids, flavonoids, tannins, and suaveolic acid. This study aims to examine the effect of H. suaveolens (L.) Poit. extract and the most effective concentration in inhibiting the growth of S. rolfsii. H. Suaveolens (L.) Poit. leaf extract was prepared by maceration method using 96% ethanol. The leaf extract solution was evaporated using a vacuum rotary evaporator. The concentration used is 0% (control), 5%, 10%, 15%, and 20% with 4 repetitions. The data obtained were analyzed using one way ANOVA and Duncan test. The results showed that H. suaveolens (L.) Poit.leaf extract could inhibit the growth of S. rolfsii and the most effective concentration was at concentration 15% with 58% inhibition percentage.
Analysis of turmeric extract (cucurmadomestica) as natural preservative of tofu to SGOT levels and hepatic tissue structure of male white rats (rattusnorvegicus) wistar strain
Authors: Widiyanti N L P M , Santiasa I M P A , Citrawathi D M , Ristiati N P , Mulyadiharja S , Rupini C I D
Published: November 2019

The purposes of this research were: (1) to determine levels of SGOT (Serum Glutamate Oxaloacetate Transferase) of wistar rats after being given preserved tofu using water/aquadest, formalin, and turmeric extract with various concentrations, and (2) to identify histopathology images of rat liver after being given tofu which is preserved using formalin, turmeric extract, and soaked with water/aquadest. This true experimental research was designed with Randomized Post Test Only Control Group Design. The sample used was male white rat (Rattusnorvegicus) wistar strain of 40 individuals. SGOT levels were analysed using one way ANOVA statistic test and histopathology images of rat liver were analysed descriptively. The results of this research show that: (1) there has been different SGOT levels among male white rat (Rattusnorvegicus) wistar strain due to preserved tofu using turmeric extract withvarious concentrations of 20%, 40%, and 60%, (2) hepatic tissue structure of rats that is given tofu with turmeric extract 40% and 60% suffered from structural damage of its liver, while the rats given with tofu with turmeric extract 20% obtained a normal liver cell structure.
Production of N2O, CO2 gases and microbe responses in the soil amended with urea granulated zeolite
Authors: Jumadi Oslan , Pagoga Selvi , Rachmawaty  , Hala Yusminah , Hiola St. Fatmah , Karim Hilda , Syahruddin Kaseng Ernawaty , Inubushi Kazuyuki
Published: November 2019

This research study aims to determine the production of nitrous oxide (N2O), carbon dioxide (CO2), the rate of ammonium-nitrate, microbe responses in soil following the addition of urea coated by zeolite under soil incubated. The result showed that urea granulated with zeolite in soil sample had a significant effect on the production of CO2and have no significant increase N2O productions. Therefore, the addition of urea with zeolite seems to decrease the production of N2O compared to urea alone. The concentrations of ammonium and nitrate during incubation time were significantly affected by the amended type of fertilizers. The availability of ammonium and nitrate in soil were increased by addition of urea zeolite 30%. The urea combinedzeolite increases the growth of the fungi population, while population of ammonium oxidizer bacteria seems appearedlower in soil that amended with fertilizer compared to the control. The study showed that urea granulated with zeoliteincreased the time availability of soil nitrogen.
Diversity of medicinal plants for pregnancy and postpartum care of Dayak Ngaju tribe in Mantangai sub-district, Kapuas regency, Central Kalimantan
Authors: Rohmat S , Nisyawati  , Rahayu S E
Published: November 2019

Traditional community still maintain their knowledge in utilizing plants for curing various of disease. The study of medicinal plants in Dayak Ngaju tribe was conducted from February – May 2017 in Mantangai sub-district. The aim of this study was to obtain new information of medicinal plants diversity for pregnancy and postpartum care. The ethnomedicinal data were gathered through open ended interview, semi-structure interview, participatory observation, and field survey. Data were analysed by Index of Cultural Significance (ICS), to determine the important of medicinal plants. The study recorded 57 medicinal plants species 36 family, which were utilized for pregnancy and postpartum care. Curcuma domestica and Piper longum has the highest value of ICS. Dayak Ngaju tribe used medicinal plants for the treatment of pregnancy and postpartum in the single or mixed herbs. They used that directly by eat the parts of plants, drink as beverages, and by steam bath.
Genotyping SNP rs7903146 TCF7L2 gene for detection T2DM in Indonesian melayu ethnic
Authors: Syamsurizal S , Handayani D , Kadri H , Badriyya E
Published: November 2019

The prevalence of T2DM is quite high in Indonesian Melayu ethnic. T2DM is a metabolic disorder that is difficult to cure but can be inhibited by its expression. T2DM expression inhibited by knowing its genetic markers. The strong genetic marker for T2DM and widely studied in other countries is SNP rs7903146 TCF7L2 gene. The aim of the research was to identify SNP rs7903146 in TCF7L2 gene as a genetic marker of T2DM in Indonesian Melayu ethnic. Identification of SNP rs7903146 TCF7L2 uses direct DNA sequencing methods and then bioinformatics analysis. SNP rs7903146 in TCF7L2 gene can be a genetic marker of T2DM in Indonesian Melayu ethnic.
Lawsonia inermis linnaeus leaf ethyl acetate extract evaluation on the kidneys of rats
Authors: Widyawati Tri , Syarifah Siti , Rita Anggraini Dwi , Sari Wahyuni Arlinda , Indah Sari Mutiara , Feriyawati Lita
Published: November 2019

Leaf extract of Lawsonia inermis Linn. leaf extract (EAE) showed the ability to decrease the high of blood glucose level and to protect the hepar of diabetic rats at dose 1 g/kgbw. The aim of the present study was to evaluate the effect of EAE on normal rats organs, namely the kidneys. EAE was obtained by serial extraction using n-hexane and ethyl acetate (EAE). 10 rats were divided into two groups that treated with EAE 1.25 g/kgbw (Group I) and distilled water (DW) 10 ml/kgbw (Group II) as control group, orally, once daily for 14 days. After 14 days the rats were sacrified for histopathological evaluation of kidney using hematoxyilline-eosine staining. Grossly, no difference was found on the kidney of DW- or EAE-treated groups. However, microscopically, kidney of DW-treated group showed normal structure of glomerulus and tubulus renalis, whereas EAE-treated group showed the congestion of tubulus renalis. The present study concluded that EAE 1,25 g/kgbw caused the inflammation of the kidney.
Coriandrum sativum l. (apiaceae) and elettaria cardamomum (l.) maton (zingiberaceae) for antioxidant and antimicrobial protection
Authors: Handayani Windri , Yasman  , Yunilawati Retno , Fauzia Vivi , Imawan Cuk
Published: November 2019

Spice plants are known for their compounds that are useful as foods flavoring, food preservatives, and medicines. This due to the presence of secondary metabolite compounds in plants such as terpenoids, flavonoids, phenols, and saponins. These compounds are known to be potential to inhibit microorganism’s growth causing decay in food and oxidation. The use of these sources for applications in the food sector is relatively safer and environmentally friendly than the use of antibiotics in general. This study was conducted to determine the antimicrobial and antioxidant activities from Coriandrum sativum L. (coriander) and Elettaria cardamomum (L.) Maton (cardamom). The essential oil extract from these plants was tested for phytochemical content qualitatively for terpenoid screening and by using Gas Chromatography-Mass Spectroscopy (GC/MS). Furthermore, the antioxidant activity from the oil extracts was tested by DPPH method. Meanwhile, their ability to inhibit gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli were tested by paper disc method. The phytochemical characterization showed a positive result of terpenoid and GC/MS result showed dominant of monoterpenes compounds, such as α-pinene and ß-pinene. The DPPH results revealed that the essential oils have different antioxidant and antimicrobial potential, whereas Coriander tends to have a higher antimicrobial activity, while Cardamom superior in antioxidant activity. These results will become the basis for the development of potential essential oil with the best antimicrobial activity for food active packaging materials.
Plants used in the traditional ceremony in kanagarian tiku
Authors: Des M , Rizki  , Fitri Melisa
Published: November 2019

The use of plants in traditional tribal ceremonies is a culture. Kanagarian Tiku are inhabited by 3 tribes, namely Minangkabau, Java and Nias. In carrying out the Traditional Ceremony in Kanagarian Tiku each tribe still uses traditional plants. The traditional ceremonies examined are traditional ceremonies of marriage, birth, death and the first milestone. The purpose of this research is to find out the type of plant, the part used and its function in traditional ceremonies of each tribe. Data collection with survey methods, interviews and plant collections. Respondents interviewed were ninik mamak, tribal chiefs, bundo kanduang and people who used to look for plants for traditional ceremonies. The results showed that 44 plant species were used in traditional ceremonies and belonged to 27 families. Parts of plants used by roots, stems, leaves, flowers, fruits and seeds. The most widely used part of the plant leaves 36.4% and the least seeds are only 1%. At the Minangkabau and Javanese tribal wedding ceremonies using 7 species, the Nias tribe is 5 species. In traditional birth ceremonies and the first milestone only performed by Minangkabau tribes using 20 species and 13 species. In the traditional ceremony the death of the Minangkabau tribe uses 10 species, Java 9 species and Nias 6 species. Plants that have the highest utilization rates are found in Piper betle, Areca chatecu, Uncaria gambir, and Nicotiana tabacum with UV 0.33.
Optimization of pectin extraction from kepok banana peels (musa paradisiaca) using surface response methodology
Authors: Pagarra H , Hartati  , Purnamasari A. B , Rachmawaty  , Rahman Roshanida A.
Published: November 2019

The experimental design in this study was used to optimize the results of extracting pectin from kepok banana peels by surface method. The model for predicting and optimizing the process of extracting kepok banana pectin consists of 4 axillary points, 4 factorial points, and 5 middle titlication replications using a central commision design (CCD) with DX6.0.4 software. The independent variables used in the optimization of the extraction results of kepok banana pectin are pH and temperature, where the pH is between 1.5 - 2.5 and temperatures are 60°C-100°C and the combination of independent and bound variables (extraction time). The results of pectin extract ranged from 5.79% to 22.57% (b / b, based on the dry weight of kepok banana peel). The variables of pH and temperature, the interaction between pH and temperature, and the interaction between extraction time and pH significantly influence. The optimum conditions for extraction of pectin are estimated at pH (2.5) and temperature (100°C). In optimal conditions, the actual pectin yield is 22.57%, which is below the estimated extraction condition of 21.286%. Analysis of variance, adj R2 and R2, model lack of fit test, and p value statistically indicate that the model is adequate in representing experimental data. The effect of pH and extraction temperature was very significant (P <0.001) on the extraction results of kepok banana peel pectin. This shows that these two variables are very important in the process of extracting kepok banana peel, where the R2 value is 96.01%.