bluuebunny/crossref_metadata_2025 huggingface dataset:
Features of obtaining malt with use of aqueous solutions of organic acids
Authors: Pivovarov O. , Kovaliova O. , Khromenko T. , Shuliakevych Z.
Published: December 2017

Recently, the traditional formulations of essential food products are actively including malt – a valuable dietary product rich in extractives and hydrolytic enzymes, obtained by germination in artificially created conditions. Containing a full set of essential amino acids and a high saccharifying ability of malt, obtained from grain cereals, determines its wide use in the production of beer, alcohol, mono- and poly-malt extracts, bakery products, special types of flour, food additives, cereals, non-alcoholic beverages, lactic acid products and, in particular, in the production of natural coffee substitutes. However, the classical germination technology, which includes 2-3 days of soaking and 5-8 days of germination due to the considerable duration and laboriousness of the process, does not meet the requirements of modern technology and the constantly growing rates of industrial production, so this problem requires finding new and improving existing scientific and technical solutions. The features of malt production using organic acids of different concentrations are presented. The malt production technology has been analyzed and investigated. It includes washing, disinfection, air and water soaking of grains, germination and drying. The feature of the technology under investigation is using of aqueous solutions of butadiene, 3-pyridinecarboxylic acid and pteroylglutamic acid. The results of the inquiry of the effect of these organic acids on energy and the ability of germination of the grain are presented. The optimal values of concentrations of active substances in solutions are revealed. The influence of organic acids on the absorption of grain moisture has been investigated. It has been established that in comparison with the classical technology, the use of these acids as a growth stimulator can reduce the overall length of the reproduction process of the material from 1.5 to 2 times and increase the yield of flour grains in the batch of malt.
The influence of antioxidant postharvest treatment on content of biologically active substances during storage of cucumbers
Authors: Priss O. , Yevlash V. , Zhukova V. , Ivanova I.
Published: December 2017

The influence of heat treatment with antioxidant compositions on the content of biologically active substances during storage of cucumbers is investigated. It was found that the use of the proposed treatment inhibits the activity of ascorbate oxidase by 15–18%, which allows to slow down the decomposition of ascorbic acid by 1.5 times. The content of ascorbic acid in the experimental fruit after storage for 28 days is 1.4 times higher than in the control after 21 days of storage. It was found that in the cucumbers 11–14 sm long, the synthesis of polyphenols continued in the period of storage.  The intensity of accumulation of phenolic substances proved to be different in the treated and control groups. It was shown that thermal treatment with antioxidants slowed the growth rate of total polyphenols by 20% relative to control, indicating inhibition of maturation processes. It was revealed that the activity o polyphenol oxidase during the storage of treated fruits was slowing down. It has been established that the application of said treatment substantially inhibits the degradation of chlorophylls. Rapid loss of chlorophyll caused yellowing and loss of consumer properties in the control groups after 2 weeks of storage. In processed cucumbers only 21 days of storage revealed a decrease in the concentration of chlorophyll in relation to the moment of laying. At day 28 the amount of chlorophylls in the experimental fruit is at the same level as in the control after a week of storage. It has been found that the use of the proposed treatment slows the degradation of carotenoids. The quantity of carotenoids in experimental cucumbers at the end of storage is 21–23% higher than in the control ones. The compensatory function of ascorbic acid, carotenoids and phenolic substances during storage of cucumber fruits is proved.
The prospects of using milk whey enriched with Mg and Mn in the technology of bakery products
Authors: Kochubei-Lytvynenko О. , Bilyk E.
Published: December 2017

In this article the technological properties of milk whey, enriched with magnesium and manganese particles by electrical discharge dispersion of metal granules in the medium, and the effect of whey on the technological process and quality of bakery products have been examined. The results of theoretical and experimental studies of the quality of milk whey enriched with Mg and Mn has been presented. The toxicological studies has determined that the enrichment of milk whey with magnesium and manganese particles has not caused potentially harmful factors that could significantly affect the viability of cells of the HEK-293, L-929, PTP lines and their morphological features. The cells of the HEK-293 line were the most vulnerable to the effect of the whey, both untreated and treated by electrical discharge method, due to the low pH of the studied whey samples. The positive influence of enriched whey if applied in the amount of 15% to the mass of flour, on the physical, chemical and sensory qualities of bakery products, particularly the increase specific volume and shelf-life, has been proven. Much attention has been paid to the staling processes, associated with the retrograding and aging of the main biopolymers of bakery products. The relevance of the presented research is in expanding the possibilities of using enriched milk whey for bakery products for the elderly.
Контамінація м’яса тварин і птиці та засоби її зниження
Authors: Палій А.П. , Родіонова К.О. , Палій А.П.
Published: December 2017

У статті представлено результати з визначення рівня мікробної контамінації м’яса забійних тварин та птиці в процесі його технологічної переробки. Визначено, що кількість МАФАнМ та бактерій родини Enterobacteriaceae на поверхні туш яловичих варіює протягом робочого часу. Найнижче значення МАФАнМ реєструють після початку роботи забійного цеху – (2,85±0,03)×103 КУО/см2, а найбільше значення реєструють під час забою у другу половину робочого часу на ділянці нутрування туш – (5,6±0,06)×103 КУО/см2. Бактерії родини Enterobacteriaceae займають вагоме місце в складі загальної кількості бактерій, які контамінують контактні поверхні яловичих туш. Середня кількість МАФАнМ в повітрі на дільниці забою та переробки птиці наприкінці робочої зміни перевищує встановлену норму у 3,3 рази, та не відповідає встановленим нормам у камері охолодження. На всіх ділянках первинної переробки м’яса птиці в пробах-змивах виявляли бактерії групи кишкової палочки. Причиною незадовільного санітарно-гігієнічного стану туш може бути порушення виробничої санітарії.  Препарат «П3-оксонія актив 150», до складу якого у якості діючих речовин входить надоцтова кислота та перекис водню, забезпечує повне знищення мікроорганізмів на поверхні тушок курчат-бройлерів при використанні 0,03 % розчину засобу за експозиції 30 хвилин, а також забезпечує мікробіологічну стійкість продукції протягом 9 діб.
The use of bacconcentrate Herobacterin in brine cheese technology
Authors: Slyvka: I. , Tsisaryk O. , Musiy L.
Published: December 2017

In the article a comparative analysis of the use of the bacterial preparation Herobacterin and the starter RSF-742 (Chr. Hansen, Denmark) in the technology of brine cheese was conducted. Herobacterin is a bacterial preparation created using bacteria Lactococcus lactis, Lactobacillus plantarum, Enterococcus faecium, Leuconostoc mesenteroides and Lactococcus garvieae, isolated from traditional Carpathian brine cheese brynza and identified using classical microbiological and modern molecular genetic methods (RAPD-PCR, RFLP-PCR, sequencing of the 16S rRNA gene). The results of investigations of organoleptic, physico-chemical, syneretical and microbiological parameters of cheese brynza with use of preparation Herobacterin are presented in comparison with the starter RSF-742, which includes cultures: Lactococcus lactis subsp. сremoris, Lactococcus lactis subsp. lactis, Streptococcus thermophilus, Lactobacillus helveticus. The use of Herobacterin has a positive effect on organoleptic, physico-chemical and microbiological parameters, all parameters complied with the requirements of DSTU 7065:2009. The level of survival of lactic acid bacteria in brynza during maturation and storage is high, which confirms the correctness of the selection of strains to preparation Herobakterin, which demonstrated good adaptability to the composition and properties of ewe's milk.
Thе оrgаnіс prоduсtіоn іn thе соntеxt оf іmprоvіng thе есоlоgісаl safety оf prоduсtіоn оf the fооd іndustry
Authors: Nikoliuk O. , Grуshоva І. , Shеstаkоvskа T.
Published: December 2017

Thе аrtісlе prеsеnts the dаtа оn thе rеlеvаnсе оf conducting sсіеntіfіс rеsеаrсh оn thе оrgаnіс prоduсtіоn іn Ukrаіnе аnd іts rоlе іn еnsurіng еnvіrоnmеntаl sаfеty in the food industry. The аnаlysіs оf оrgаnіс аgrісulturаl prоduсtіоn іn thе wоrld аnd Ukrаіnе hаs bееn саrrіеd оut. Іt іs еstаblіshеd thаt іn Ukrаіnе thеrе іs nо stаtе suppоrt fоr thе dеvеlоpmеnt оf thе оrgаnіс sесtоr, hоwеvеr, іt оссupіеs lеаdіng pоsіtіоns іn dіffеrеnt grоups оf сulturеs іn Еurоpе аnd іn thе wоrld аs а whоlе. Іt іs substаntіаtеd thаt аgrісulturаl lаnds must mееt сеrtаіn rеquіrеmеnts rеgаrdіng thе lеvеl оf thеіr соntаmіnаtіоn by hаrmful substаnсеs, hеаvy mеtаls, rаdіоnuсlіdеs, еtс., fоr thе purpоsе оf соnduсtіng оrgаnіс fаrmіng аnd еnsurіng еnvіrоnmеntаl sаfеty of the products of the food industry. Thіs suggеsts thаt thе conversion pеrіоd fоr thе trаnsіtіоn tо оrgаnіс, еnvіrоnmеntаlly safe аgrісulturаl prоduсtіоn іs ассоmpаnіеd by іndіvіduаl rіsks, whісh nесеssіtаtеs thе sоlutіоn оf thе соrrеspоndіng tаsks, whісh аrе systеmаtіzеd іntо соmplеx grоups. Thе conducted аnаlysіs оf tесhnоlоgіеs usеd іn thе prоduсtіоn оf оrgаnіс аgrісulturаl prоduсts usеd іn thе PP "Аgrоесоlоgy" hаs shоwn thаt thеy аrе соst-еffесtіvе, еnvіrоnmеntаlly sаfе, еnеrgy аnd rеsоurсе-sаvіng, аnd аrе сhаrасtеrіzеd by sосіаl оrіеntаtіоn. Strаtеgіс dіrесtіоns оf prоvіdіng оf есоlоgісаl sаfеty of production of оn thе food industry bаsіs оf lоng-tеrm dеvеlоpmеnt оf оrgаnіс prоduсtіоn іn Ukrаіnе аrе оffеrеd.
Collaborative procurement of the European Spallation Source in Sweden
Authors: Stenman Lennart , Larsson Jan-Anders , Wade Steve
Published: December 2017

The European Spallation Source is a €1·8 billion neutron source being built at Lund in Sweden. On completion in 2023 it will be the most powerful in the world, providing a giant ‘molecular microscope’ for studying all kinds of natural and man-made materials. This paper describes the collaborative procurement approach adopted in the delivery of this highly complex project. In particular it allowed construction to start before the scientific design and radiation safety analysis was finalised, helping to ensure delivery on time and within budget.
Core strength of concrete using newly developed in situ compressive testing machine
Authors: Reddy Bijivemujla Sudheer Kumar , Wanjari Swapnil
Published: December 2017

Core tests are commonly used for the verification of the in situ compressive strength of concrete. Laboratory core testing (LCT) takes up to 5 d. A newly developed in situ compressive testing (ICT) machine, which reduces the time required for core testing from 4–5 d to 30 min by testing cores on site, was developed. Comparative studies were carried out between the proposed ICT and LCT on 54 core samples of 24 mm diameter and 36 samples each of 42 mm and 67 mm diameter with various height to diameter (H/D) ratios. Student's t-tests between the core strength results obtained by LCT and ICT indicated no significant difference between the two methods. The core strength was found to increase with a decrease in H/D ratio for a given core diameter, and the core strength increased with an increase in diameter for a given H/D ratio. The standard deviation of the results was found to increase with a decrease in core diameter. The same trends were observed in both LCT and ICT results, indicating that the proposed ICT machine can be used for testing concrete strength.
Assessment of SFRC flat slab punching behaviour – part II: reversed horizontal cyclic loading
Authors: Gouveia Nuno D. , Faria Duarte M. V. , Ramos António Pinho
Published: December 2017

This paper presents an experimental study of four flat slab specimens subjected to combined vertical and horizontal cyclic loading. Steel fibre-reinforced concrete (SFRC) was used only in the local region of the slab–column connection, while the rest of the slabs were cast using normal concrete. The specimens measured 4·15 m × 1·85 m × 0·15 m and were connected to two steel half columns by 0·25 m × 0·25 m rigid steel plates, prestressed against the slab using steel bolts, to ensure monolithic behaviour. The specimens were tested using an innovative test setup system that accounted for important factors, such as the ability of bending moment redistribution, line of inflection mobility and assured equal vertical displacements at the opposite slab borders, and symmetrical shear forces. Results show that the presence of SFRC in the slab–column connection region is effective in increasing the deformation capacity of slab–column connections, allowing the increase of horizontal drift ratios.
Pathogenicity of a Human Laminin β2 Mutation Revealed in Models of Alport Syndrome
Authors: Funk Steven D. , Bayer Raymond H. , Malone Andrew F. , McKee Karen K. , Yurchenco Peter D. , Miner Jeffrey H.
Published: December 2017

Pierson syndrome is a congenital nephrotic syndrome with eye and neurologic defects caused by mutations in laminin β2 (LAMB2), a major component of the glomerular basement membrane (GBM). Pathogenic missense mutations in human LAMB2 cluster in or near the laminin amino-terminal (LN) domain, a domain required for extracellular polymerization of laminin trimers and basement membrane scaffolding. Here, we investigated an LN domain missense mutation, LAMB2-S80R, which was discovered in a patient with Pierson syndrome and unusually late onset of proteinuria. Biochemical data indicated that this mutation impairs laminin polymerization, which we hypothesized to be the cause of the patient’s nephrotic syndrome. Testing this hypothesis in genetically altered mice showed that the corresponding amino acid change (LAMB2-S83R) alone is not pathogenic. However, expression of LAMB2-S83R significantly increased the rate of progression to kidney failure in a Col4a3
−/− mouse model of autosomal recessive Alport syndrome and increased proteinuria in Col4a5
+/− females that exhibit a mild form of X-linked Alport syndrome due to mosaic deposition of collagen α3α4α5(IV) in the GBM. Collectively, these data show the pathogenicity of LAMB2-S80R and provide the first evidence of genetic modification of Alport phenotypes by variation in another GBM component. This finding could help explain the wide range of Alport syndrome onset and severity observed in patients with Alport syndrome, even for family members who share the same COL4 mutation. Our results also show the complexities of using model organisms to investigate genetic variants suspected of being pathogenic in humans.
Construção e aplicação de um modelo tridimensional como recurso didático para o ensino de síntese proteica
Authors: Lazzaroni Alberto Alexandre , Agate Platais Brasil Teixeira Gerlinde
Published: December 2017

Muitos temas de Biologia são complexos e abstratos. Assim, o uso de modelos capazes de simplificar sem, no entanto, banalizar o conhecimento são importantes ferramentas para a transposição do saber acadêmico no âmbito escolar. Entendendo a necessidade de sua construção e, ao mesmo tempo, diante da carência de um modelo concreto que exemplifique a síntese proteica, o objetivo deste trabalho foi a elaboração do referido modelo. Para tanto, foi aplicado o método ativo de ensino através da proposição de um desafio: a concepção de um modelo feito pelos alunos de Ensino Médio. Uma vez construído o protótipo, a estratégia foi aplicada a licenciandos de Biologia, que o avaliaram e criticaram. Nossos resultados mostram que a tática utilizada foi eficaz. Verificou-se, desse modo, que o modelo deva ser reproduzido e que sua aplicação facilitou o aprendizado tanto dos secundaristas como dos graduandos. Concluímos que a disponibilidade do modelo deve contribuir para o aprendizado de bioquímica / biologia molecular na educação formal.
Clinical and epidemiological features of visceral leishmaniasis in the Republic of Crimea
Authors: Baranets M S , Ermak T N , Ponirovsky E N
Published: December 2017

The paper analyzes official statistical data, as well as visceral leishmaniasis (VL) cases who have visited the Clinical Center, I.M. Sechenov First Moscow State Medical University, and the results of parasitological studies of the materials submitted to the Reference Center for Monitoring of Leishmaniasis, E.I. Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases. Account is taken of the observations made by the Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, and of the literature data available in Russian and foreign (Ukraine) scientific journals and monographs. During the period of 1932 to 2015, a total of 14 VL cases acquired in the Crimea were notified, of which there were 4 cases of VL concurrent with HIV infection. The majority of infections occurred in the eastern part of the peninsula. Given that many areas of Crimea have the status of recreation and annually receive millions of vacationists, it is necessary to conduct a more in-depth study of all aspects of this infection.
Evolution of laboratory markers for systemic bacterial infections
Authors: Ryabkova N L , Vezikova N N
Published: December 2017

The paper reviews the data available in the literature on existing laboratory markers for systemic bacterial infection, among which C-reactive protein, proinflammatory cytokines, procalcitonin test, and presepsin receive primary emphasis.
Schnitzler syndrome: A review of literature, the authors’ observation, and experience with the interleukin-1 inhibitor canakinumab
Authors: Salugina S O , Gorodetsky V R , Fedorov E S , Lopatina N E , Evsikova M D
Published: December 2017

The paper reviews the publications dealing with Schnitzler syndrome, a rare autoinflammatory disease, and describes the authors’ own clinical observation. It describes the first Russian experience in successfully using the interleukin-1 inhibitor canakinumab to treat this disease.
Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B
Authors: Kostyushev D S , Zueva A P , Brezgin S A , Lipatnikov A D , Simirskii V N , Glebe D , Volchkova E V , Shipulin G A , Chulanov V P
Published: December 2017

Aim. To define the role of DNA-methyltransferases of type 1 and type 3A in hepatitis B viral cycle. Materials and methods. Human hepatoma cells HepG2 with stable expression of 1.1-mer HBV genome were transfected with vectors encoding DNA-methyltransferase 1 (DNMT1), DNA-methyltransferase 3A (DNMT3A) or were co-transfected with these vectors. Total HBV DNA copy number, relative expression of pregenomic RNA (pgRNA), S-protein-encoding RNA (S-RNA) and cccDNA were analyzed by quantitative and semi-quantitative real-time PCR-analysis with TaqMan probes for assessment of DNMTs-mediated effects on HBV. Results. DNMT1 and DNMT3A suppress HBV transcription and replication, though to different magnitude. cccDNA pool is enlarged statistically significantly ≈2-fold (P
Impact of current approaches to laboratory screening of donated blood and its components on hepatitis B virus infection in patients with blood system diseases
Authors: Ignatova E N , Tupoleva T A , Ovchinnikova E N , Romanova T Yu , Yaroslavtseva N G , Filatov F P , Troitskaya V V , Kuzmina L A , Parovichnikova E N , Gaponova T V , Savchenko V G
Published: December 2017

Aim. To evaluate the detection rate of markers for hepatitis B virus (HBV) in the blood samples taken from patients with blood system diseases, by applying the current approaches to examining donated blood and its components for markers of viral infections. Materials and methods. The investigation included blood samples from patients with blood system diseases (n=364) and donors (n=5,011). The results of laboratory screening of donated blood samples (n=13,081) were retrospectively analyzed. Commercial kits of reagents were used for immunochemical assay and polymerase chain reaction. Results. Patients with blood system diseases were recorded to have markers of active HBV infection in 12.6% of cases, anti-HBc in 31.3%, and anti-HBs in 37.6%. A retrospective analysis of the results of screening donated blood samples showed the presence of markers for active HBV infection in 0.28% of cases. A prospective examination of blood donors revealed markers of HBV infection in 4.83% of cases, including those of active forms in 0.54% and anti-HBc in 4.79%. The markers of active HBV infection in donors were only anti-HBc IgM in 0.42% of cases. The blood samples from donors with an anti-HBs titer of >200 mIU/ml contained anti-HBc IgM in 10.5%. Conclusion. In the last 5-7 years, the detection rate of markers of HBV infection in the blood samples of patients with blood system diseases have remained at a high level. Screening for decreed markers fails to identify people with inapparent infections among the donors. Even high anti-HBs concentrations in the donated blood may be a risk for HBV transmission by transfusion to a recipient.
Necrotizing sarcoid granulomatosis with clinical presentations of recurrent acute abdomen. Case report and literature review
Authors: Vasilyev V I , Palshina S G , Chaltsev B D , Radenska-Lopovok S G , Safonova T N
Published: December 2017

The authors have described the world’s first case of necrotizing sarcoid granulomatosis (NSG) in a 22-year-old woman with the clinical presentations of acute abdomen, which are associated with abdominal lymph nodal infiltration and necrosis, obvious constitutional disturbances (fever, nocturnal sweats, and significant weight loss), high inflammatory activity (anemia, leukocytosis, high erythrocyte sedimentation rates and C-reactive protein levels), the gradual appearance of splenic and hepatic necrotic foci, and infiltration into the lung and lacrimal glands with the development of unilateral uveitis. The patient underwent five surgical interventions, several needle biopsies for recurrent abdominal syndrome, and long-term antibiotic treatment for presumed sepsis, which had caused drug-induced hepatitis. Bacteriological examination of blood, puncture samples, and removed abdominal cavity tissues, serological tests, and immunomorphogical study of biopsy samples and removed tissues yielded negative results for the presence of bacterial, fungal, and tuberculosis infections. NSG was diagnosed on the basis of the systemic nature of the lesion, the presence of granulomas with severe abdominal lymph nodal necrosis and necrotizing granulomatous/lymphocytic vasculitis in the mesentery and removed spleen, as well as the absence of granulomas in the spleen, appendix, and biopsy materials of the liver, colonic mucosa, and parotid gland. Fludarabine therapy was first used in world practice due to the inefficient treatment with high-dose glucocorticoids and cyclophosphamide and to a disease relapse when reducing their doses. The paper gives a detailed review of the literature on the clinical, laboratory, radiological, and morphological manifestations of the disease, which allow the differential diagnosis of NSG with different variants of granulomatous lesions. Based on the 5-year follow-up of the patient and on the analysis of clinical, laboratory, radiological, and morphological changes, the authors uphold the concept that the disease is an independent nosological entity: necrotizing angiitis with sarcoid reactions, rather than the entity of nodular or classic sarcoidosis.
Difficulties in the diagnosis of diarrhea-associated hemolytic uremic syndrome in adults
Authors: Malov V A , Maleev V V , Kozlovskaya N L , Tsvetkova N A , Smetanina S V , Gorobchenko A N , Serova V V , Chentsov V B , Volkov A G , Faller A P
Published: December 2017

Hemolytic uremic syndrome (HUS) is a rare, but menacing condition registered mainly in children. The paper gives a detailed description and analysis of a clinical case of HUS with a favorable outcome in an adult woman who developed the syndrome in the presence of bloody diarrhea. It considers an update on the etiology, pathogenesis, and clinical features of HUS associated with diarrheal syndrome and discusses differential diagnostic features, diagnostic problems, and characteristics of management tactics for patients.
The virus-immune hypothesis for cardiac dilatation
Authors: Kozhevnikov M L , Shipulin V M , Sukhodolo I V
Published: December 2017

The paper gives an update on the pathogenetic role of viral infection and immune mechanisms in the development of cardiac dilatation at the cellular, ultrastructural, and molecular levels. Particular attention is given to the discussion of the possible role of herpesvirus infection in the mechanisms of cardiomyocyte damage with the direct or indirect impact of viral infection through immunoinflammatory responses. Data on the protective and damaging action of a number of cytokines in the immunopathogenesis of viral myocarditis are considered.
PREGNANT AWARENESS ABOUT ZIKA VIRUS INFECTION DURING PREGNANCY
Authors: Costa Taís Paiva da , Nunes Junior David da Costa
Published: December 2017

Introduction: The infection by Zika virus (ZIKV) on pregnancy has been related to several birth defects such as congenital microcephaly and fetal deaths. According to what is currently known about this infection, the only way to avoid it is prevention. Objective: Evaluate the pregnant women awareness about usual ZIKV infection risks. Methods and materials: This is a quantitative descriptive observational report developed at a outpatient clinic in Salvador/BA. The analysis group was composed by 60 pregnant subjects of usual risk from September to October of 2016. The data acquisition was done by structured instruments such as questions regarding sociodemographic data and ZIKV infection risks. Results: Approximately 97% of the subjects affirmed to known how the disease spread occurs, and all of them have mentioned the Aedes aegypti mosquito. The most commonly reported indications and symptoms were maculopapular rash (77.8%), headache (66.7%) and pruritus (61.1%). The most frequent complication was microcephaly (98.3%). About ways of prevention from the disease, 93.3% affirmed that they are aware of the virus. The use of the repellent lotion was the most mentioned (96.5%). Just 51.8% claimed to always do some prevention measures in their daily basis. And 88.6% of the subjects who had unplanned pregnancy allege that they would not wish to become pregnant with real chances of being infected by the virus. Conclusion: the studied group is aware of the infection risks by ZIKV, but there is a lack of prevention acts.
Using a new tool to evaluate the functional capacity of patients with HTLV-1 associated myelopathy/Tropical spastic paraparesis (HAM/TSP)
Authors: Rocha-Santos Isabelle , Boa-Sorte Ney , Rios Grassi Maria Fernanda , Couto de Sá Cloud , Araújo Thessika Hialla , Galvão Castro Bernardo
Published: December 2017

HAM/TSP patients experience impaired functional capacity.  The use of categorical scales to accurately describe motor ability in these patients has been questioned.Aim: To evaluate the functional capacity of HAM/TSP patients by using the individual tests comprising the GDLAM protocol.Methods: Cross sectional study carried out in the CHTLV, Salvador, Brazil.  Functional capacity was evaluated using the tests of the GDLAM protocol (timed 10-minute walk, getting up from a sitting position, rising from a prone position, getting up from a chair, and moving around the house). Functional classifications were made by comparing patient performance to that of uninfected control individuals.Results: Twenty-four HAM/TSP and 25 healthy individuals were enrolled. Only 75% HAM/TSP patients completed the GDLAM protocol. Six patients failed to perform the RPP test. Timed measurements were significantly higher in the HAM/TSP patients in comparison to uninfected controls.Conclusion: The use of alternative methods, such as the individual tests comprising the GDLAM protocol, may more accurately assess motor function in HAM/TSP patients.
SYSTEMIC ARTERIAL HYPERTENSION: SOCIAL REPRESENTATIONS OF PATIENTS ATTENDED AT PRIMARY CARE ABOUT THE DISEASE AND ITS TREATMENT
Authors: Brito Sabrina Nogueira , Fraga-Maia Helena Maria Silveira , Zarife André
Published: December 2017

Introdução: Hipertensão Arterial Sistêmica (SAH) é considerado um problema de saúde pública muito relevante, devido à sua alta freqüência na população em todo o mundo. As Representações Sociais (SR) foram abordadas em estudos com o objetivo de compreender a relação do aspecto cultural e psicossocial com os meios de lidar com pessoas com SAH, justificando e orientando suas atitudes e expectativas em relação à sua condição. Objetivo: o objetivo era compreender SR de pacientes com Hipertensão Arterial Sistêmica em relação ao tratamento, adesão terapêutica e fatores de risco para complicações. Estratégia metodológica:Uma abordagem qualitativa foi utilizada de acordo com a perspectiva Minayo e entrevistas guiadas por um roteiro semi-estruturado como técnica de pesquisa em uma Unidade de Saúde da Família em Salvador-BA. Resultados: um total de 18 informantes-chave com idade variando de 32 a 72 anos participaram do estudo. Em geral, os usuários eram principalmente sedentários (83,4%) e tinham sobrepeso ou obesidade (44,5%). Os dados foram organizados em três fases para análise: ordenação, classificação e análise final, permitindo a determinação do priori e do emic. Os indivíduos relataram dificuldades quanto à necessidade de ingestão diária de medicamentos e à adoção de hábitos de vida saudáveis, sugerindo diferenças entre senso comum e conhecimento técnico.  Considerações finais:Múltiplas influências foram percebidas sobre as crenças individuais e as redes sociais na adesão ao tratamento. A SR dos hipertensos interfere diretamente com sua percepção sobre a doença, orientando-os em seus comportamentos de saúde.
Assentamento Monjolinho: Indicadores para o entendimento da permanência de famílias assentadas
Authors: Santos Daiane Santana , Guimarães Valter
Published: December 2017

O presente trabalho teve como objetivo central, investigar sobre a permanência de famílias assentadas pelo projeto de reforma agrária no Assentamento Monjolinho, localizado no município de Anastácio-MS. Inicialmente foi realizada a pesquisa da literatura pertinente, tanto de caráter geral relacionada à reforma agrária, quanto aquelas de caráter regional e local. Foram realizadas visitas a trinta e duas propriedades, utilizando-se de aplicação de questionários, visitas in loco na Unidade Avançada do Instituto Nacional de Colonização e Reforma Agrária (INCRA) em Jardim-MS, e na Unidade da Agência de Desenvolvimento Agrário e Extensão Rural (AGRAER) de Anastácio-MS. Os resultados obtidos permitiram a elaboração de gráficos, quadro, e a análise destes, além de contribuir no enriquecimento do embasamento teórico. Foi possível considerar que a permanência dessas famílias está ligada a uma afetividade que foi construída forçosamente ao longo do tempo, pois os mesmos já estavam há anos batalhando para conseguir um pedaço de terra e quando a receberam, se depararam com imensas dificuldades, mas como não tinham outra saída, e como já sempre trabalharam na “roça”, optaram por permanecer no local.

PALAVRAS-CHAVE: Assentamento Monjolinho. Permanência de Famílias. Reforma Agrária.
Indicadores de consumo de água, vazamentos e perdas: estudo de caso das escolas públicas de Recife
Authors: Nunes Luiz Gustavo Costa Ferreira , Wanderley Thayná Russo de Barros , Silva Simone Rosa da
Published: December 2017

O presente artigo objetiva através de instrumentos de gestão hídrica analisar o consumo da água em prédios públicos escolares do município de Recife, Pernambuco. A metodologia utilizada foi o cálculo de indicadores de consumo, vazamentos e perdas em nove escolas-piloto, calculados através de levantamento do consumo com a concessionária local, e levantamentos de dados em campo. As médias dos indicadores de consumo das escolas variaram entre 7,27 e 37,96 L/aluno/dia para o ano de 2016. As escolas apresentaram uma média de 13% no indicador de vazamentos, e podem representar aproximadamente até 13% do consumo de água. Percebe-se que uma política de inspeção e manutenção periódica das instalações hidrossanitárias consistiria em um importante instrumento no combate às perdas por vazamentos, que consequentemente implicaria em redução do consumo de água, conservação do insumo e redução de custos, além dos benefícios ambientais.

PALAVRAS-CHAVE: conservação de água; vazamentos; escolas públicas.
Potencial de Substituição de Cimento por Finos de Quartzo em Materiais Cimentícios
Authors: Damineli Bruno Luis , Travain Maysa Damante
Published: December 2017

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Destinação de resíduos sólidos e líquidos em propriedades rurais do município de Monções - SP
Authors: Carvalho Sérgio Luís de , Peroni Neto Orestes , Fávero Miguel Spolon
Published: December 2017

A questão da temática ambiental tem sido cada vez mais discutida e vem sendo o centro das atenções no Brasil e no mundo nos últimos anos. O presente trabalho teve o objetivo de avaliar alguns aspectos das condições ambientais de propriedades rurais do município de Monções – SP. Foi aplicado um questionário que foi usado para entrevistar 45 produtores, contendo 26 questões com diversas respostas, as quais abordaram possíveis problemas ambientais e como eles eram tratados pelos proprietários rurais do município. As respostas obtidas permitiram diagnosticar diversos problemas ambientais em relação ao descarte de embalagens de agrotóxicos, uso de EPIs e destino de resíduos sólidos e líquidos de origem doméstica e agrícola. Foram detectados problemas diversos, entre eles a utilização de agrotóxicos sem os equipamentos de proteção individual, o descarte incorreto do lixo e das embalagens de agrotóxicos e o lançamento de esgoto em fossas negras, os quais podem causar contaminações ao meio ambiente e doenças às pessoas, necessitando-se, portanto, de amplos programas de educação ambiental voltados para comunidade agropecuária de forma a manter de forma sustentável as suas atividades.

PALAVRAS-CHAVE: condições ambientais. Agrotóxicos. sustentabilidade
Interferência do volume de aplicação e adjuvantes nos depósitos da pulverização em plantas de feijão
Authors: Azevedo Maurício Gomes , Omoto Pedro Hideki , Prado Evandro Pereira
Published: December 2017

Adjuvantes são substancias adicionadas na calda de pulverização cuja finalidade é melhorar a eficiência das aplicações de defensivos agrícolas. A pesquisa teve como objetivos avaliar diferentes classes de adjuvantes agrícolas e volume de aplicação sobre os depósitos da pulverização em plantas de feijão. O ensaio para determinação do efeito do adjuvante na retenção da calda de pulverização foi realizado em estufa e o ensaio visando à interferência do volume de aplicação em condições de campo. Resultados distintos em relação à deposição da calda em plantas de feijão foram encontrados a depender do tipo de adjuvante utilizado. O adjuvante Haiten aplicado na dose de 100 mL 100L-1 reduziu os valores de deposição e o adjuvante Agral aplicado na dose de 50 mL 100L-1 aumentou esses valores. O aumento do volume de aplicação proporcionou maiores valores de deposição nas plantas de feijão, porém esse aumento foi significativo até o volume de 160 L ha-1. Na medida em que se aumentou o volume de aplicação foi verificado aumento dos valores de deposição na entre linha das plantas. O mesmo foi observado nas linhas das plantas até a aplicação no volume de 160 L ha-1. A presença do adjuvante na calda de pulverização não influenciou a deposição na linha e na entre linha da cultura do feijão.
PALAVRAS-CHAVE: Tecnologia de aplicação, contaminação por defensivos, retenção foliar.
Avaliação do desempenho da utilização de areia de fundição em argamassas por meio de ensaios de resistência à compressão
Authors: Berti João Vitor Meneguetti , Batista João Pedro Bittencourt , Akasaki Jorge Luis
Published: December 2017

A construção civil movimenta grande parte da economia brasileira. O concreto é o principal material utilizado neste setor e para produzi-lo é necessário explorar grandes quantidades de recursos naturais, prejudicando o meio ambiente. Com isso, gera-se a necessidade de desenvolver materiais alternativos para substituir materiais constituintes do concreto e argamassa, como a areia natural. A areia de fundição, resíduo gerado das caldeiras de biomassa para produção de energia, é um material que pode ser utilizado como alternativa para minimizar o consumo de areia natural. A proposta deste trabalho é analisar a substituição em massa da areia natural em argamassas com 25, 50, 75 e 100% com relação cimento/areia 1:2 e relação água/cimento de 0,5. As argamassas foram submetidas ao ensaio de resistência de compressão aos 7, 28 e 90 dias de cura, além da determinação do índice de consistência, segundo as normas vigentes da Associação Brasileira de Normas Técnicas (ABNT). Para análise comparativa dos resultados, foram feitas amostras controles com 100% de areia natural. Para a idade de 7 dias todas as amostras com substituição em massa ficaram pouco abaixo do controle, porém para 28 e 90 dias, com substituição de 25 e 50%, as amostras obtiveram resistência igual ou superior ao controle. Portanto, o estudo mostrou que a areia de fundição é uma alternativa viável economicamente e sustentável.

PALAVRAS-CHAVE: Construção civil. Sustentabilidade. Areia de fundição.
Vantagens e Desvantagens do Escritório em Edificação Térrea e em Edifício Alto: Um Estudo Exploratório
Authors: Miskalo Anne , Schmid Aloísio Leoni
Published: December 2017

O objetivo deste estudo é avaliar, de forma preliminar, as vantagens e desvantagens do escritório em edificação térrea e em edifício alto, por meio da experiência vivida dos participantes da pesquisa; comparar os resultados entre os dois tipos de edificação; bem como avaliar, corrigir e complementar o questionário aplicado, com o propósito de validar o instrumento de pesquisa, a ser utilizado em futuros estudos. Este é um estudo exploratório, no qual realizou-se a coleta de dados por intermédio de um questionário misto, aplicado em 39 estudantes universitários do Programa de Pós-Graduação em Engenharia de Construção Civil da Universidade Federal do Paraná, na cidade de Curitiba-PR, Brasil. Nos resultados, verificou-se nas respostas mais citadas, como vantagens do escritório em edificação térrea: fácil acesso, sem escadas e elevadores, facilidade de fuga e estacionamento; e como desvantagens: ruído, insegurança, visitas indesejadas, alto fluxo de pessoas, cinza dos fumantes e distração. Nas respostas mais citadas, como vantagens do escritório em edifício alto: vista, menos ruído, ventilação, segurança, fluxo de pessoas, status e tranquilidade; e como desvantagens: necessidade de escadas e elevadores, dificuldade de fuga, difícil acesso, dificuldade de locomoção e estacionamento. Além disso, observou-se pela comparação dos resultados da pesquisa, que foram citadas mais vantagens e menos desvantagens do escritório em edifício alto em relação ao escritório em edificação térrea.

PALAVRAS-CHAVE: Escritório. Edificação térrea. Edifício alto.
Universal DNA isolation protocol v1
Authors: Kalendar Ruslan
Published: December 2017

The isolation of nucleic acids from a sample is an important step for many molecular biological applications and medical diagnostic assays. This prptpcoldescribes an efficient method for purification or/and isolation of nucleic acids from difficult animal tissues, plant material and other samples from which DNA extraction is generally regarded as being difficult which can contain impurities and inhibitors or interfering substances. This method is established acidic CTAB (with a pH value of 4 to 5.5) based extraction protocol that allows for reliable isolation of high molecular weight genomic DNA for removing contaminants from nucleic acids in a sample, e.g., environmental or biological samples such as soil, food, plant, animal or microorganism. DNA may quickly be extracted from samples without phenol, guanidine thiocyanate or 2-mercaptoethanol.
Yeast-Sucrose-Broth v1
Authors: known Not
Published: December 2017

Preparation of Yeast-Sucrose-Broth, that is used as liquid media for growingZt.This is called 'YSB' in Bruce McDonald lab.Have a look for example: Zain, M. E., et al. 'Influence of growth medium on diagnostic characters of Aspergillus and Penicillium species.'African Journal of Microbiology Research3.5 (2009): 280-286.
Territorialidades en conflicto y acciones colectivas: las luchas por el agua en Sierras Chicas, provincia de Córdoba, Argentina
Authors: Chiavassa Sergio , Ensabella Beatriz , Deón Joaquín Ulises
Published: December 2017

Las localidades ubicadas en el faldeo oriental de las Sierras Chicas de Córdoba, sufren cíclicamente crisis por insuficiencia hídrica y también inundaciones. En este escenario, se vienen planteando situaciones conflictivas entre diversos agentes sociales con intereses contrapuestos en torno al agua, el uso del suelo y el bosque. La situación ha provocado movilizaciones vecinales que requieren de un análisis territorial en el abordaje de los conflictos ambientales. El artículo propone analizar las acciones colectivas de las organizaciones sociales de Sierras Chicas de Córdoba que vienen trabajando en torno a las problemáticas ambientales regionales. Para ello, analizamos las cuencas como territorios y abrimos el debate sobre recursos naturales y bienes comunes de la naturaleza. Siguiendo a Escobar (2010) y Babiano-Amelibia (2015) el propósito último es pensar en posibilidades de construir otros modelos de democracia y otras alternativas al desarrollo centradas en las lógicas del lugar.
Cambios y continuidades de la gestión de las redes de agua en el área metropolitana de Buenos Aires
Authors: Catenazzi Andrea C.
Published: December 2017

Este artículo se propone examinar las relaciones de mutua transformación que se establecen entre las redes técnicas y el territorio metropolitano. El análisis se centra en las continuidades y rupturas de la gestión del agua en el área metropolitana de Buenos Aires. Para dar cuenta de esa problemática, se efectúa un análisis de los cambios institucionales de la empresa prestadora de los servicios de agua y desagüe cloacal desde principios del siglo XX hasta la actualidad. La hipótesis que orienta el estudio sostiene que esos cambios institucionales incluyeron una creciente territorialización de la cuestión del acceso al agua desplegada en tensión con la gestión sectorial y centralizada heredada de Obras Sanitarias de la Nación.
Politización de la crisis hídrica en Chile: Análisis del conflicto por el agua en la provincia de Petorca
Authors: Panez-Pinto Alexander , Faúndez-Vergara Rodrigo , Mansilla-Quiñones Camilo
Published: December 2017

El artículo tiene como objetivo analizar los procesos de politización de los conflictos por el agua en Chile a partir del estudio de caso de la provincia de Petorca (región de Valparaíso) y, particularmente, el surgimiento y trayectoria del Movimiento por la Defensa del Agua, la Tierra y el Medioambiente (MODATIMA). Las principales contribuciones del análisis de dicha experiencia tienen que ver con: a) Los alcances del proceso de politización de este tipo de conflictos en su cuestionamiento al modelo de desarrollo chileno. b) La relación de esta politización con la reivindicación de otras miradas sobre la comprensión del agua. c) La vinculación de la conflictividad en torno al agua con los procesos de acumulación de capital por desposesión que se experimentan en Chile y a nivel global.
Suburban Infill to Redevelop Suburbia—Retrofitting Post-Suburban Business Locations in the Zürich Metropolitan Area as a Component of Growth Management
Authors: Jansen Hendrik
Published: December 2017

Regional growth management has become a significant component of sustainable urban land development in many European metropolitan areas. Particularly in rapidly growing metropolitan regions, increasing population and job numbers require strategic planning management, but there is little knowledge about which planning instruments influence or direct these processes most effectively. Based on an analysis of spatial development in the Zürich metropolitan region, particularly in suburban areas, over the past several decades, this article examines the opportunities for the infill, revitalization and retrofitting of suburban business locations as key elements of growth management. In doing so, this article focuses on one central question: To what extent does (cantonal) regional planning and its specific instruments (cantonal structure plan) influence and control the spatial development and urban design quality in the retrofitting of suburban locations? The Glattal region was chosen as a case study as it experienced a significant change in terms of its urban structure during the last 10–15 years. In this context, suburban service locations were examined and analysed in depth using two case studies. The Hochbord area in Dübendorf and the Glattpark area in Opfikon demonstrate the conversion of formerly mono-functional areas (Hochbord) to mixed-use neighbourhoods and the development of new mixed-use locations (Glattpark). The article demonstrates how the suburban office stock transformed to strategic spots for mixed-use in both locations and explores how the retrofitting process could be directed at the cantonal level. In this context, spatial planning instruments at the regional level, such as the cantonal structure plan, seem to play a significant role in the transformation of the urban periphery.
Agency in Professional Self-Determination of Adolescentswith Special Social Situations of Development
Authors: Aron I.S.
Published: December 2017

The article explains the importance of agency in professional identity formation as a conscious, independent, active, responsible, professional choice relevant in modern society. Psychological readiness for agency is considered its integral indicator which includes cognitive, activity, motivational, and personality components. The paper presents outcomes of a research on the specifics of psychological readiness to professional self-determination in adolescents with special social situations of development. This research involved 211 adolescents including orphans, teenagers with deviant behavior, and gifted teenagers. A set of techniques used in the research consisted of five standard techniques and a questionnaire developed by the author. The obtained results revealed that there is a lack of readiness for professional self-determination in all adolescent groups: orphans and teenagers with deviant behaviors showed insufficient rates for all four components of readiness, while gifted adolescents showed low rates for activity and personal components. The results of this research may help develop differentiated, person-centered technologies for promoting agency in professional self-determination of adolescents with special social situations of development. The author links the prospects of further investigations with the study of positive characteristics of psychological readiness of young people with special social situations of development to professional self-determination; these characteristics can serve as a compensatory and developmental potential of agency in professional self-determination in the context of specific social contexts and limited agency-based resources of professional self-determination.