bluuebunny/crossref_metadata_2025 huggingface dataset:
Impaired renal function is an independent risk factor for complications after surgery for femoral neck fracture
Authors: Ramamurti Pradip , Fassihi Safa C , Sacolick David , Gu Alex , Wei Chapman , Chodos Marc D
Published: June 2021

Background:

The metabolic abnormalities that occur secondary to chronic kidney disease (CKD) increase the risk of femoral neck fractures compared to the general population. The purpose of this study is to determine whether impaired renal function is an independent risk factor for complications after surgery for femoral neck fracture.

Methods:

The ACS-NSQIP database was reviewed for patients who underwent total hip arthroplasty, hemiarthroplasty and open reduction internal fixation (ORIF) for femoral neck fractures between 2007 and 2018. Patients were split into cohorts based on calculated estimated glomerular filtration rate. Demographic information, comorbidities, and 30-day complications were analysed with univariate and multivariate analyses using chi-square, Fischer’s exact and analysis of variance testing.

Results:

The total number of patients for the study was 163,717. Patients with CKD stage 4 and 5 had an increased rate of any complication (39.1 and 36.7% respectively) compared with higher eGFRs ( p  < 0.001). Similarly, 30-day mortality was increased at 6.0% and 6.7% for both stage 4 and 5 ( p  < 0.001). By multivariate regression, those with CKD Stage 4 and 5 were at increased risk for any complication compared to patients with a normal preoperative eGFR of 90–120 ( p  < 0.001).

Conclusions:

This study demonstrated that patients with CKD Stage 4 and 5 are at increased risks of all complications, including death, renal, pulmonary and thromboembolic disease. Therefore, these patients should be cared for from a multidisciplinary approach with close attention to postoperative medications and fall prevention to help mitigate the risk of complications in the immediate postoperative period.
In Silico Integrative Approach Revealed Key MicroRNAs and Associated Target Genes in Cardiorenal Syndrome
Authors: Ahmed Mohd Murshad , Ishrat Romana , Tazyeen Safia , Alam Aftab , Farooqui Anam , Ali Rafat , Imam Nikhat , Tamkeen Naaila , Ali Shahnawaz , Malik Md Zubbair , Sultan Armiya
Published: June 2021

Cardiorenal syndromes constellate primary dysfunction of either heart or kidney whereby one organ dysfunction leads to the dysfunction of another. The role of several microRNAs (miRNAs) has been implicated in number of diseases, including hypertension, heart failure, and kidney diseases. Wide range of miRNAs has been identified as ideal candidate biomarkers due to their stable expression. Current study was aimed to identify crucial miRNAs and their target genes associated with cardiorenal syndrome and to explore their interaction analysis. Three differentially expressed microRNAs (DEMs), namely, hsa-miR-4476, hsa-miR-345-3p, and hsa-miR-371a-5p, were obtained from GSE89699 and GSE87885 microRNA data sets, using R/GEO2R tools. Furthermore, literature mining resulted in the retrieval of 15 miRNAs from scientific research and review articles. The miRNAs-gene networks were constructed using miRNet (a Web platform of miRNA-centric network visual analytics). CytoHubba (Cytoscape plugin) was adopted to identify the modules and the top-ranked nodes in the network based on Degree centrality, Closeness centrality, Betweenness centrality, and Stress centrality. The overlapped miRNAs were further used in pathway enrichment analysis. We found that hsa-miR-21-5p was common in 8 pathways out of the top 10. Based on the degree, 5 miRNAs, namely, hsa-mir-122-5p, hsa-mir-222-3p, hsa-mir-21-5p, hsa-mir-146a-5p, and hsa-mir-29b-3p, are considered as key influencing nodes in a network. We suggest that the identified miRNAs and their target genes may have pathological relevance in cardiorenal syndrome (CRS) and may emerge as potential diagnostic biomarkers.
From compassion to distance: Hannah Höch’s ‘Mother’
Authors: Pérez-Fernández Andrea
Published: June 2021

This article addresses the work of the German artist Hannah Höch in the light of the struggle for abortion rights in the Weimar Republic. I attempt to show how Höch’s uses of the technique of photomontage can be read as a way of introducing a distance between the work and the viewer that allows us to question the beliefs we use to make sense of the world. Specifically, I discuss her photomontage Mutter (‘Mother’), a version of a photograph taken by John Heartfield, and some of her writings and interviews. I also examine closely the material conditions and political debates in which Höch’s work – as a social practice – developed. After a brief introduction and a methodological outline, I present Höch in the context of Berlin Dada and summarise the main underlying arguments of my hypothesis. Namely, that the major interest of Höch’s photomontages lies in the complex articulation of activism and philosophy, and in the way in which they put mainstream categories into question by ‘distancing’ fragments of reality.
Professional activists? Party activism among political staffers in parliamentary democracies
Authors: Moens Pieter
Published: June 2021

Although the position of the party on the ground has been weakened by cartelization, grassroots activists remain an important recruitment pool for political professionals. Based on unique survey data collected among the staff of 14 Belgian and Dutch parties (N = 1009), this article offers an in-depth analysis of party activism among this under-researched population. Introducing a new supply and demand framework, I argue that staff recruitment is shaped by candidate preferences (supply) and party preferences (demand). The findings demonstrate that most political staffers are high-intensity activists with a strong commitment to their party. Moreover, the theoretical model accurately predicts that non-activists are more common among policy and communication experts, ministerial staff, and those working for ideologically moderate parties. These findings show that paid staffers do not necessarily widen the gap between parties and activists. They also raise normative questions about internal congruence within parties in coalition governments.
Illiberalism and the democratic paradox: The infernal dialectic of neoliberal emancipation
Authors: Swyngedouw Erik
Published: June 2021

The main trust of this article unfolds around the impasse of democratic politics today, marked by the fading belief in the presumably superior architecture of liberal democratic institutions to nurture emancipation on the one hand, and the seemingly inexorable rise of a variety of populist political movements on the other. The first part of the article focuses on the lure of autocratic populism. The second part considers how transforming neoliberal governance arrangements pioneered post-truth autocratic politics/policies in articulation with the imposition of market rule and, in doing so, cleared the way for contemporary illiberal populisms. The third part considers the institutional configuration through which the democratic has been fundamentally transformed over the past few decades in the direction of a post-democratic constellation. The article concludes by arguing for the need to re-script emancipation as a process of political subjectivation unfolding trough a political act.
The politics of prison air: Breath, smell, and wind in Myanmar prisons
Authors: Martin Tomas Max
Published: June 2021

This article explores what air means and entails in penal settings and examines how carcerality attaches itself to air. With inspiration from social science approaches to the study of air, I propose that the lived experience of prison air can be fruitfully analyzed through the notions of breath, smell, and wind. This point is explored through two incidents about prison air drawn from ethnographic fieldwork in Myanmar. Together they illustrate a shift in Myanmar penality from a martial logic of destroying the enemy towards an internationally infused rationality of control and care. The first is a tale of an imprisoned engineer’s subversive effort to improve the air quality in prisons; the second, the design and building of tuberculosis wards in prisons that aim to bring prison air in line with international standards. The analysis of these incidents broadens the analytical sensorium of prison air by drawing attention to, on the one hand, a basic empirical and affective sensing of air, recognising air as a scarce and coveted resource that prison actors’ appropriate to survive. On the other hand, attention is drawn to the possibility of sensing with air, whereby the discourses, technologies, rules, and practices of air can be utilized as entry points for the analysis of prison governance and the transitional dynamics of penality.
Classification revisited: On time, methodology and position in decolonizing anthropology
Authors: Pels Peter
Published: June 2021

Renewed calls for decolonizing anthropology in the 21st century raise the question of what work earlier waves of decolonization since the 1960s have left undone. Some of this work should focus on the classification of human differences, which figured prominently in all phases of the discipline’s history: as a methodology in its racist phases, as an object of study during its late colonial phase of professionalization, as self-critical reflexivity in the 1980s and 1990s, and as a renewed critique in the 21st century. Can a universal methodology of studying classifications of human kinds arise from the discipline’s past of colonial stereotyping? I argue affirmatively, through an approach that recognizes time as the epistemic condition that connects past and present positions to present and future methodologies. Firstly, my analysis distinguishes the parochial embedding in colonial culture of Durkheim and Mauss’ ideas about classification from their more universal intentions. This is then developed into a threefold reflexive and timeful methodology of studying classification’s nominal-descriptive, constructive, and interventionist dimensions—a process of adding temporality to the study of classification. Subsequently, Anténor Firmin’s 19th-century critique of racial classifications, and W. E. B. Du Bois’s theory of double consciousness help to show how this threefold methodology addresses the insufficiently theorized process of being classified and discriminated against through racial categories wielded by the powers that be. These arguments radicalize the essay’s timeful perspective by concluding that we need to avoid modernist uses of time as classification and adopt the aforementioned threefold methodology in order to put time in classifications of human kinds. This reverses modern positivism’s subordination to methodological rules of the epistemic conditions posed by contingent history and shows instead that the universal goals of methodology should be understood as a future ideal.
Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma
Authors: Li Ruolin , Chen Gang , Dang Yiwu , He Rongquan , Liu Angui , Ma Jie , Ling Zhian
Published: June 2021

Multiple myeloma (MM) is one of the main blood disorders threatening human health today. This study aimed to examine the expression of BCL-2/adenovirus E1B 19 kDa-interacting protein 3-like (BNIP3L) in patients with MM and explore its mechanisms in silico. Bone marrow samples (n = 36 from patients with MM and n = 12 from healthy donors) were used to conduct BNIP3L expression analysis using immunohistochemistry. Microarray or RNA sequencing data from the Sequence Read Archive, Gene Expression Omnibus, and ArrayExpress databases were used to appraise BNIP3L expression and its prognostic role in patients with MM. The co-expressed genes of BNIP3L were identified for enrichment and protein-protein interaction (PPI) analyses to determine the associated signaling pathways. Immunohistochemistry indicated that BNIP3L expression in bone marrow of patients with MM was significantly lower than that in bone marrow of healthy donors. BNIP3L mRNA expression was also significantly lower in patients with MM than in healthy donors. The overall standard mean difference (SMD) for downregulation of BNIP3L was −0.62 [−1.17, −0.06], and the area under the curve was 0.81 [0.78, 0.85] based on a total of 694 MM cases. The overall survival analysis demonstrated that BNIP3L levels could act as an independent protective indicator of MM patient survival (HR = 0.79). Moreover, 261 co-expressed genes of BNIP3L were confirmed and found to be mainly involved in the adipocytokine signaling pathway. We preliminarily proved that downregulation of BNIP3L may play an important role in the occurrence and development of MM, and the promoting cancer capacity may be related to the pathway of adipocytokine signaling pathway.
Can the Risk of Dysphagia in Head and Neck Radiation Therapy Be Predicted by an Automated Transit Fluence Monitoring Process During Treatment? A First Comparative Study of Patient Reported Quality of Life and the Fluence-Based Decision Support Metric
Authors: Lim Seng Boh , Lee Nancy , Zakeri Kaveh , Greer Peter , Fuangrod Todsaporn , Coffman Frederick , Cerviño Laura , Lovelock D. Michael
Published: June 2021

Purpose/Objective(s):

The additional personnel and imaging procedures required for Adaptive Radiation Therapy (ART) pose a challenge for a broad implementation. We hypothesize that a change in transit fluence during the treatment course is correlated with the change of quality of life and thus can be used as a replanning trigger.

Materials/Methods:

Twenty-one head and neck cancer (HNC) patients filled out an MD Anderson Dysphagia Inventory (MDADI) questionnaire, before-and-after the radiotherapy treatment course. The transit fluence was measured by the Watchdog (WD) in-vivo portal dosimetry system. The patients were monitored with daily WD and weekly CBCTs. The region of interest (ROI) of each patient was defined as the outer contour of the patient between approximate spine levels C1 to C4, essentially the neck and mandible inside the beam’s eye view. The nth day integrated transit fluence change, Δϕn, and the volume change, ΔVROI, of the ROI of each patient was calculated from the corresponding WD and CBCT measurements. The correlation between MDADI scores and age, gender, planning mean dose to salivary glands , weight change ΔW, ΔVROI, and Δϕn, were analyzed using the ranked-Pearson correlation.

Results:

No statistically significant correlation was found for age, gender and ΔW.  was found to have clinically important correlation with functional MDADI (ρ = −0.39, P = 0.081). ΔVROI was found to have statistically significant correlation of 0.44, 0.47 and 0.44 with global, physical and functional MDADI ( P-value < 0.05). Δϕn was found to have statistically significant ranked-correlation (−0.46, −0.46 and −0.45) with physical, functional and total MDADI ( P-value < 0.05).

Conclusion:

A transit fluence based decision support metric (DSM) is statistically correlated with the dysphagia risk. It can not only be used as an early signal in assisting clinicians in the ART patient selection for replanning, but also lowers the resource barrier of ART implementation.
Exploring the Classification Accuracy of the Early Communication Indicator (ECI) With Dual-Language Learners From Latinx Backgrounds
Authors: King Marika , Larson Anne L. , Buzhardt Jay
Published: June 2021

Few, if any, reliable and valid screening tools exist to identify language delay in young Spanish–English speaking dual-language learners (DLLs). The early communication indicator (ECI) is a brief, naturalistic measure of expressive communication development designed to inform intervention decision-making and progress monitoring for infants and toddlers at-risk for language delays. We assessed the accuracy of the ECI as a language-screening tool for DLLs from Latinx backgrounds by completing classification accuracy analysis on 39 participants who completed the ECI and a widely used standardized reference, the Preschool Language Scales, Fifth Edition–Spanish, (PLS-5 Spanish). Sensitivity of the ECI was high, but the specificity was low, resulting in low classification accuracy overall. Given the limitations of using standalone assessments as a reference for DLLs, a subset of participants ( n = 22) completed additional parent-report measures related to identification of language delay. Combining the ECI with parent-report data, the specificity of the ECI remained high, and the sensitivity improved. Findings show preliminary support for the ECI as a language-screening tool, especially when combined with other information sources, and highlight the need for validated language assessment for DLLs from Latinx backgrounds.
Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer
Authors: Yu Jianfa , Lang Qi , Zhong Chongli , Wang Shuang , Tian Yu
Published: June 2021

Background:

Autophagy plays a vital role in cancer development. However, there is currently no comprehensive study regarding the effects of autophagy-related genes (ARGs) on pancreatic cancer prognosis. Thus, this study aimed to establish an autophagy-related signature for predicting the prognosis of patients with pancreatic cancer.

Methods:

We identified and validated differentially-expressed ARGs using data from The Cancer Genome Atlas (TCGA) database, Genotype-Tissue Expression project (GTEx) and Expression Omnibus (GEO) database. We performed Cox proportional hazards regression analysis on the differentially-expressed ARGs to develop an autophagy-related signature. We tested the expression of these genes through western blotting and verified their prognostic values through gene expression profiling and interactive analyses (GEPIA).

Results:

We identified a total of 21 differentially-expressed ARGs and screened 4 OS-related ARGs (TP63, RAB24, APOL1, and PTK6). Both the training and validation sets showed that the autophagy-related signature was more accurate than the Tumor Node Metastasis (TNM) staging system. Moreover, the western blotting result showed that the expression of TP63, APOL1, and PTK6 was high, whereas that of RAB24 was low in cancer tissues.

Conclusion:

This 4-ARG signature might potentially help in providing personalized therapy to patients with cancer.
Numerical analysis on the performance characteristics of a new gas journal bearing by using finite difference method
Authors: Li Yuntang , Li Ruirui , Ye Yueliang , Li Xiaolu , Chen Yuan
Published: June 2021

This paper proposes a novel gas journal bearing in which orifices are different in diameter and distribute unevenly. Finite Difference Method (FDM) combined with Linear Perturbation Method (LPM) is used to solve the unsteady-state Reynolds equation of the flow field in the bearing clearance. Moreover, four types of bearing structures are used to discuss the effects of orifices different in diameter and uneven distribution on the bearing performance. The results demonstrate that the new bearing has better static and dynamic performances compared with those of traditional bearing in which orifices are equal in diameter and distribute evenly. Moreover, thin gas film thickness, high supply pressure, and large eccentricity ratio are hopeful for improving load capacity of the new bearing. Furthermore, the stability of the novel bearing is improved if eccentricity ratio is 0.25–0.3.
Effect of relaxation and retardation times on dusty Jeffrey fluid in a curved channel with peristalsis
Authors: Khan Ambreen Afsar , Zafar Saira , Kanwal Atifa
Published: June 2021

In recent work, the Jeffrey liquid with uniform dust particles in a symmetric channel is studied. Moving sinusoidal wave is executed on the walls of the channel, which generates peristaltic transport in the fluid. The governing equations for fluid and dust particles have been formulated using stream function. Perturbation method is used to get analytical solution of the problem by using small wave number. Graphical analysis has been carried out for stream function and velocity of fluid and dust particles. Effects of different parameters such as curvature k, relaxation time [Formula: see text], wave number [Formula: see text] and retardation time [Formula: see text] are debated through graphs for both dust particles and fluid. The noteworthy outcomes are fluid velocity, pressure gradient in the region [Formula: see text] and bolus size increases by increasing [Formula: see text]
Applying theory to overcome internal barriers for healthy behavior change in adults with intellectual disabilities
Authors: Oliver Amy , Munk Niki , Stanton-Nichols Kathleen A
Published: June 2021

Adults with disabilities are 57% less physically active than individuals without disabilities and two times as likely to be obese. With obesity, adults with disabilities also face increased risk of comorbid disabilities stemming from obesity. The purpose of this theoretical case study was to identify key behavioral change theories which may be utilized to increase physical activity levels in adults with intellectual disabilities. The Self-Efficacy Theory and Self-Determination Theory both present constructs for understanding behavior change, and many of these constructs are interrelated which strongly suggests many behavioral change theories identify internal barriers for change. With theoretical case studies, these theories are examined within the context of adapted physical activity to depict how the Self-Efficacy Theory and Self-Determination Theory could be utilized to increase physical activity in individuals with intellectual disabilities.
Improving outcomes in germ cell cancers using miRNA
Authors: Conduit Ciara , Tran Ben
Published: June 2021

Owing to advances in treatment paradigms across the last five decades, testicular cancer is now eminently curable. However, current serum tumour and imaging biomarkers lack adequate sensitivity, specificity, and predictive value. Subsequently, their utility in detecting active malignancy and informing treatment decisions is minimal in a large proportion of men with testicular cancer. Micro-ribonucleic acids (miRNA), pertinently miR-371a-3p, offer a new tool, which based on early data, appears to fill many of the gaps that existing biomarkers leave. This paper reviews the evolution of the technology, potential limitations, and discusses the clinical relevance of miRNA as it moves towards the clinic.
Virgin Coconut Oil Resists Arsenic-Induced Cerebral Neurotoxicity and Cholesterol Imbalance via Suppression of Oxidative Stress, Adenosine Deaminase and Acetylcholinesterase Activities in Rats
Authors: Azubuike-Osu Sharon O. , Famurewa Ademola C. , David Japheth C. , Abi Innocent , Ogbu Patience N. , Oparaji Chiedozie K. , Nwaeze Konyefom G. , Akunna Godson G.
Published: June 2021

Arsenic (As) is a classic neurotoxicant; its pathogenesis is associated with oxidative stress and oxidative stress-mediated cholinergic deficits. This study explored antioxidant activity of virgin coconut oil (VCO) against sodium arsenite-induced oxidative stress-mediated cerebral neurotoxicity in rats. Eighteen rats were divided into 3 groups- Normal control, As control and VCO + As. The VCO (5 mL/kg) was given once daily by oral gavage from day 1 to day 21, while As (10 mg/kg) was given once daily by oral gavage from day 15 to day 21. Cerebral superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), malondialdehyde (MDA), adenosine deaminase (ADA) and acetylcholinesterase (AchE) activities were analysed. Nitric oxide (NO), lipid profile, phospholipid (PL), and reduced glutathione (GSH) were also evaluated in cerebral homogenate. The cerebrum was sectioned for histological analysis. Administration of As induced significant depressions in antioxidant enzymes, GSH, PL, and HDL-c compared to normal control. Levels of MDA, NO, total cholesterol and activities of ADA, AchE in the cerebrum were markedly increased by As compared to normal rats. Lipid profile indices and PL were prominently altered by As. Histopathological study supported the biochemical findings through extensive cerebral damage. In contrast, oral supplementation of VCO prior to and along with As treatment significantly attenuated the As-induced biochemical alterations and restored near-normal histology. VCO attenuates cerebral neurotoxicity by strengthening endogenous antioxidant defence and cholinergic function via counteracting free-radical-mediated arsenic toxicity.
The Perfect Postnormal Storm: COVID-19 Chronicles (2020 Edition)
Authors: Jones Christopher , Serra del Pino Jordi , Mayo Liam
Published: June 2021

This essay addresses the COVID-19 pandemic as a case study in postnormal times phenomena: a perfect postnormal storm. The essay introduces basic concepts of postnormal analysis and provides examples of the acceleration of speed, scope, scale, and simultaneity of change in a number of human and natural systems and gives examples of the accelerating complexity, chaos, and contradictions that characterize phenomenon and systems as they become more postnormal. The related concepts of the layers of ignorance and uncertainty are explored related to the movement of phenomenon toward postnormal states. The importance of the idea of manufactured normalcy fields and resistance to or accommodation of postnormal burst, such as lag and tilt, can help to better understand the postnormal landscape. For example, the pros and cons of returning to “normal” raise fundamental questions about the logic and wisdom of the dominant growth and economic paradigm.
Effects of Noise and Second Language on Conversational Dynamics in Task Dialogue
Authors: Sørensen A. Josefine Munch , Fereczkowski Michal , MacDonald Ewen N.
Published: June 2021

This study provides a framework for measuring conversational dynamics between conversational partners (interlocutors). Conversations from 20 pairs of young, normal-hearing, native-Danish talkers were recorded when speaking in both quiet and noise (70 dBA sound pressure level [SPL]) and in Danish and English. Previous studies investigating the intervals from when one talker stops talking to when the next one starts, termed floor-transfer offsets (FTOs), suggest that typical turn-taking requires interlocutors to predict when the current talker will finish their turn. We hypothesized that adding noise and/or speaking in a second language (L2) would increase the communication difficulty and result in longer and more variable FTOs. The median and interquartile range of FTOs increased slightly in noise, and in L2, there was a small increase in interquartile range but a small decrease in the median of FTO durations. It took the participants longer to complete the task in both L2 and noise, indicating increased communication difficulty. The average duration of interpausal units, that is, units of connected speech surrounded by silences of 180 ms or more, increased by 18% in noise and 8% in L2. These findings suggest that talkers held their turn for longer, allowing more time for speech understanding and planning. In L2, participants spoke slower, and in both L2 and noise, they took fewer turns. These changes in behavior may have offset some of the increased difficulty when communicating in noise or L2. We speculate that talkers prioritize the maintenance of turn-taking timing over other speech measures.
The Subaltern Critique of Dominant Historiography and a Case for Retrieving a Historiography of Dalit Literary Traditions in India
Authors: Vandana
Published: June 2021

In order to retrieve literary history in India, teleology operates on three levels: ancient, medieval and modern. As per the longue duree approach to the study of history, history is not an event or an object, but like the concept of time, is a configuration and a process. The history of the longue duree gives priority to long-term monumental historic patterns, moments and shifts in society, that is, the slow-paced structural processes which tend to have strong historical consequences. Similarly, languages and literatures, too, marked by historical catastrophes, undergo a process of sedimentation. For this reason, instead of a single literary history of South Asia, Sheldon Pollock proposes the concept of ‘literary cultures’ which allows room for ‘historical individuation’ of each culture rather than homogenising them merely for the sake of historical analysis. The basic questions that I have tried to look into through this study include: Why is it problematic to retrieve literary history in India? Why is it essential to have an alternative literary historiography of Dalit literature? How does Dalit subalternity differ from colonial subalternity? How the Dalit voice is disintegrated from within because of the prevalence of graded inequality? What constitutes the politics of history writing and canon formation in the third world countries like India where retrieving subaltern literary trends remain a problematic discourse?
Effects of Accumulated Energy on Nanoparticle Formation in Pulsed-Laser Dewetting of AgCu Thin Films
Authors: Lin H. K. , Huang C. W. , Lin Y. H. , Chuang W. S. , Huang J. C.
Published: June 2021

Ag50Cu50 films were deposited on glass substrates by a sputtering system. Effects of accumulated energy on nanoparticle formation in pulse-laser dewetting of AgCu films were investigated. The results showed that the properties of the dewetted films were found to be dependent on the magnitude of the energy accumulated in the film. For a low energy accumulation, the two distinct nanoparticles had rice-shaped/Ag60Cu40 and hemispherical/Ag80Cu20. Moreover, the absorption spectra contained two peaks at 700 nm and 500 nm, respectively. By contrast, for a high energy accumulation, the nanoparticles had a consistent composition of Ag60Cu40, a mean diameter of 100 nm and a peak absorption wavelength of 550 nm. Overall, the results suggest that a higher Ag content of the induced nanoparticles causes a blue shift of the absorption spectrum, while a smaller particle size induces a red shift.
When physical activity meets the physical environment: precision health insights from the intersection
Authors: Giles Luisa V. , Koehle Michael S. , Saelens Brian E. , Sbihi Hind , Carlsten Chris
Published: June 2021

Background

The physical environment can facilitate or hinder physical activity. A challenge in promoting physical activity is ensuring that the physical environment is supportive and that these supports are appropriately tailored to the individual or group in question. Ideally, aspects of the environment that impact physical activity would be enhanced, but environmental changes take time, and identifying ways to provide more precision to physical activity recommendations might be helpful for specific individuals or groups. Therefore, moving beyond a “one size fits all” to a precision-based approach is critical.

Main body

To this end, we considered 4 critical aspects of the physical environment that influence physical activity (walkability, green space, traffic-related air pollution, and heat) and how these aspects could enhance our ability to precisely guide physical activity. Strategies to increase physical activity could include optimizing design of the built environment or mitigating of some of the environmental impediments to activity through personalized or population-wide interventions.

Conclusions

Although at present non-personalized approaches may be more widespread than those tailored to one person’s physical environment, targeting intrinsic personal elements (e.g., medical conditions, sex, age, socioeconomic status) has interesting potential to enhance the likelihood and ability of individuals to participate in physical activity.
Near accidents and collisions between pedestrians and cyclists
Authors: Mesimäki Johannes , Luoma Juha
Published: June 2021

Cities throughout the world have increasingly promoted walking and cycling as healthy and sustainable modes of travel. However, collisions between pedestrians and cyclists have remained largely unstudied, and existing accident statistics suffer from underreporting. This study aimed to explore near accidents and collisions between pedestrians and cyclists, assess the frequency of near accidents, and evaluate pedestrians’ and cyclists’ sense of safety in traffic. An online survey was directed to inhabitants of Finnish cities with populations greater than 100,000, and the resulting data included 1046 respondents who walk and/or cycle regularly.

The main results show that near accidents between pedestrians and cyclists are around 50 times more frequent than collisions. Only 16 survey respondents had been involved in a collision during the 3-year period, whereas roughly a third had experienced at least one near accident. For both near accidents and collisions, the involved parties were usually travelling in the same direction. Most incidents occurred on pedestrian paths and shared pedestrian and bicycle paths. On shared pedestrian and bicycle paths separated by mode of transport, incidents were much rarer. Furthermore, sense of safety and willingness to walk and cycle were lower in environments where near accidents were more frequent.

These findings tentatively suggest that spatially separating modes of transport could improve people’s sense of safety and prevent near accidents and collisions. Prevention of near accidents could increase the willingness to walk and cycle.
BiGAN: LncRNA-disease association prediction based on bidirectional generative adversarial network
Authors: Yang Qiang , Li Xiaokun
Published: June 2021

Background

An increasing number of studies have shown that lncRNAs are crucial for the control of hormones and the regulation of various physiological processes in the human body, and deletion mutations in RNA are related to many human diseases. LncRNA- disease association prediction is very useful for understanding pathogenesis, diagnosis, and prevention of diseases, and is helpful for labelling relevant biological information.

Results

In this manuscript, we propose a computational model named bidirectional generative adversarial network (BiGAN), which consists of an encoder, a generator, and a discriminator to predict new lncRNA-disease associations. We construct features between lncRNA and disease pairs by utilizing the disease semantic similarity, lncRNA sequence similarity, and Gaussian interaction profile kernel similarities of lncRNAs and diseases. The BiGAN maps the latent features of similarity features to predict unverified association between lncRNAs and diseases. The computational results have proved that the BiGAN performs significantly better than other state-of-the-art approaches in cross-validation. We employed the proposed model to predict candidate lncRNAs for renal cancer and colon cancer. The results are promising. Case studies show that almost 70% of lncRNAs in the top 10 prediction lists are verified by recent biological research.

Conclusion

The experimental results indicated that our proposed model had an accurate predictive ability for the association of lncRNA-disease pairs.
Transcriptome signature of miRNA-26b KO mouse model suggests novel targets
Authors: van der Vorst Emiel P. C. , Pepe Mario A. A. , Peters Linsey J. F. , Haberbosch Markus , Jansen Yvonne , Naumann Ronald , Stathopoulos Georgios T. , Weber Christian , Bidzhekov Kiril
Published: June 2021

Background

MicroRNAs (miRNAs) are short (20–24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. One of the miRNAs that has been shown to play a role in various pathologies like cancer, neurological disorders and cardiovascular diseases is miRNA-26b. However, these studies only demonstrated rather ambiguous associations without revealing a causal relationship. Therefore, the aim of this study is to establish and validate a mouse model which enables the elucidation of the exact role of miRNA-26b in various pathologies.

Results

A miRNA-26b-deficient mouse model was established using homologous recombination and validated using PCR. miRNA-26b-deficient mice did not show any physiological abnormalities and no effects on systemic lipid levels, blood parameters or tissue leukocytes. Using next generation sequencing, the gene expression patterns in miRNA-26b-deficient mice were analyzed and compared to wild type controls. This supported the already suggested role of miRNA-26b in cancer and neurological processes, but also revealed novel associations of miRNA-26b with thermogenesis and allergic reactions. In addition, detailed analysis identified several genes that seem to be highly regulated by miRNA-26b, which are linked to the same pathological conditions, further confirming the role of miRNA-26b in these pathologies and providing a strong validation of our mouse model.

Conclusions

miRNA-26b plays an important role in various pathologies, although causal relationships still have to be established. The described mouse model of miRNA-26b deficiency is a crucial first step towards the identification of the exact role of miRNA-26b in various diseases that could identify miRNA-26b as a promising novel diagnostic or even therapeutic target in a broad range of pathologies.
Temporal dynamics of teleost populations during the Pleistocene: a report from publicly available genome data
Authors: Li Jia , Bian Chao , Yi Yunhai , Yu Hui , You Xinxin , Shi Qiong
Published: June 2021

Background

Global climate oscillation, as a selection dynamic, is an ecologically important element resulting in global biodiversity. During the glacial geological periods, most organisms suffered detrimental selection pressures (such as food shortage and habitat loss) and went through population declines. However, during the mild interglacial periods, many species re-flourished. These temporal dynamics of effective population sizes (Ne) provide essential information for understanding and predicting evolutionary outcomes during historical and ongoing global climate changes.

Results

Using high-quality genome assemblies and corresponding sequencing data, we applied the Pairwise Sequentially Markovian Coalescent (PSMC) method to quantify Ne changes of twelve representative teleost species from approximately 10 million years ago (mya) to 10 thousand years ago (kya). These results revealed multiple rounds of population contraction and expansion in most of the examined teleost species during the Neogene and the Quaternary periods. We observed that 83% (10/12) of the examined teleosts had experienced a drastic decline in Ne before the last glacial period (LGP, 110–12 kya), slightly earlier than the reported pattern of Ne changes in 38 avian species. In comparison with the peaks, almost all of the examined teleosts maintained long-term lower Ne values during the last few million years. This is consistent with increasingly dramatic glaciation during this period.

Conclusion

In summary, these findings provide a more comprehensive understanding of the historical Ne changes in teleosts. Results presented here could lead to the development of appropriate strategies to protect species in light of ongoing global climate changes.
Integrative analysis of histomorphology, transcriptome and whole genome resequencing identified DIO2 gene as a crucial gene for the protuberant knob located on forehead in geese
Authors: Deng Yan , Hu Shenqiang , Luo Chenglong , Ouyang Qingyuan , Li Li , Ma Jiaming , Lin Zhenping , Chen Junpeng , Liu Hehe , Hu Jiwei , Chen Guohong , Shu Dingming , Pan Yuxuan , Hu Bo , He Hua , Qu Hao , Wang Jiwen
Published: June 2021

Background

During domestication, remarkable changes in behavior, morphology, physiology and production performance have taken place in farm animals. As one of the most economically important poultry, goose owns a unique appearance characteristic called knob, which is located at the base of the upper bill. However, neither the histomorphology nor the genetic mechanism of the knob phenotype has been revealed in geese.

Results

In the present study, integrated radiographic, histological, transcriptomic and genomic analyses revealed the histomorphological characteristics and genetic mechanism of goose knob. The knob skin was developed, and radiographic results demonstrated that the knob bone was obviously protuberant and pneumatized. Histologically, there were major differences in structures in both the knob skin and bone between geese owing knob (namely knob-geese) and those devoid of knob (namely non-knob geese). Through transcriptome analysis, 592 and 952 genes differentially expressed in knob skin and bone, and significantly enriched in PPAR and Calcium pathways in knob skin and bone, respectively, which revealed the molecular mechanisms of histomorphological differences of the knob between knob- and non-knob geese. Furthermore, integrated transcriptomic and genomic analysis contributed to the identification of 17 and 21 candidate genes associated with the knob formation in the skin and bone, respectively. Of them, DIO2 gene could play a pivotal role in determining the knob phenotype in geese. Because a non-synonymous mutation (c.642,923 G > A, P265L) changed DIO2 protein secondary structure in knob geese, and Sanger sequencing further showed that the AA genotype was identified in the population of knob geese, and was prevalent in a crossing population which was artificially selected for 10 generations.

Conclusions

This study was the first to uncover the knob histomorphological characteristics and genetic mechanism in geese, and DIO2 was identified as the crucial gene associated with the knob phenotype. These data not only expand and enrich our knowledge on the molecular mechanisms underlying the formation of head appendages in both mammalian and avian species, but also have important theoretical and practical significance for goose breeding.
Comparative transcriptome analysis reveals distinct gene expression profiles in Brachypodium distachyon infected by two fungal pathogens
Authors: Zhu Gengrui , Gao Chengyu , Wu Chenyu , Li Mu , Xu Jin-Rong , Liu Huiquan , Wang Qinhu
Published: June 2021

Background

The production of cereal crops is frequently affected by diseases caused by Fusarium graminearum and Magnaporthe oryzae, two devastating fungal pathogens. To improve crop resistance, many studies have focused on understanding the mechanisms of host defense against these two fungi individually. However, our knowledge of the common and different host defenses against these pathogens is very limited.

Results

In this study, we employed Brachypodium distachyon as a model for cereal crops and performed comparative transcriptomics to study the dynamics of host gene expression at different infection stages. We found that infection with either F. graminearum or M. oryzae triggered massive transcriptomic reprogramming in the diseased tissues. Numerous defense-related genes were induced with dynamic changes during the time course of infection, including genes that function in pattern detection, MAPK cascade, phytohormone signaling, transcription, protein degradation, and secondary metabolism. In particular, the expression of jasmonic acid signaling genes and proteasome component genes were likely specifically inhibited or manipulated upon infection by F. graminearum.

Conclusions

Our analysis showed that, although the affected host pathways are similar, their expression programs and regulations are distinct during infection by F. graminearum and M. oryzae. The results provide valuable insight into the interactions between B. distachyon and two important cereal pathogens.
Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize
Authors: Wang Nan , Cheng Ming , Chen Yong , Liu Bojuan , Wang Xiaonan , Li Guojun , Zhou Yueheng , Luo Ping , Xi Zhangying , Yong Hongjun , Zhang Degui , Li Mingshun , Zhang Xuecai , Vicente Felix San , Hao Zhuanfang , Li Xinhai
Published: June 2021

Background

Natural variations derived from both evolutionary selection and genetic recombination, presume to have important functions to respond to various abiotic stresses, which could be used to improve drought tolerance via genomic selection.

Results

In the present study, the NAC-encoding gene of ZmNAC080308 was cloned and sequenced in 199 inbred lines in maize. Phylogenetic analysis showed that ZmNAC080308 is closely clusteredinto the same group with other well-known NAC genes responding to improve drought tolerance. In total, 86 SNPs and 47 InDels were identified in the generic region of ZmNAC080308, 19 of these variations were associated with GY (grain yield) in different environments. Nine variations in the 5’-UTR region of ZmNAC080308 are closely linked, they might regulate the gene expression and respond to improve GY under drought condition via Sp1-mediated transactivation. Two haplotypes (Hap1 and Hap2) identified in the, 5’-UTR region using the nine variations, and Hap2 containing insertion variants, exhibited 15.47 % higher GY under drought stress condition. Further, a functional marker was developed to predict the drought stress tolerance in a US maize inbred line panel. Lines carrying Hap2 exhibited > 10 % higher GY than those carrying Hap1 under drought stress condition. In Arabidopsis, overexpression ZmNAC080308 enhanced drought tolerance.

Conclusions

ZmNAC080308 is an important gene responding to drought tolerance, a functional marker is developed for improving maize drought tolerance by selecting this gene.
First case of an invasive Bacteroides dorei infection detected in a patient with a mycotic aortic aneurysm—raising a rebellion of major indigenous bacteria in humans: a case report and review
Authors: Matsuoka Takayuki , Shimizu Takuya , Minagawa Tadanori , Hiranuma Wakiko , Takeda Miki , Kakuta Risako , Kawamoto Shunsuke
Published: June 2021

Background

Bacteroides dorei is an anaerobic gram-negative bacterium first described in 2006. Because of the high similarity in mass spectra between B. dorei and Bacteroides vulgatus, discriminating between these species is arduous in clinical practice. In recent decades, 16S rRNA gene sequencing has been a complementary method for distinguishing taxonomically close bacteria, including B. dorei and B. vulgatus, at the genus and species levels. Consequently, B. dorei has been shown to contribute to some diseases, including type 1 autoimmune diabetes mellitus and atherosclerotic diseases. However, there are no reports on invasive infectious diseases caused by B. dorei. This report describes the first case of direct invasion and colonisation of human tissue by B. dorei, thus providing a warning regarding the previously proposed application of B. dorei as a live biotherapeutic for atherosclerotic diseases.

Case presentation

A 78-year-old Japanese man complained of intermittent chest/back pain and was diagnosed with a mycotic thoracic aortic aneurysm by enhanced computed tomography on admission. Despite strict blood pressure control and empirical antibiotic therapy, the patient’s condition worsened. To prevent aneurysmal rupture and eliminate infectious foci, the patient underwent surgical treatment. The resected specimen was subjected to tissue culture and 16S rRNA gene sequencing analysis to identify pathogenic bacteria. A few days after the surgery, culture and sequencing results revealed that the pathogen was B. dorei/B. vulgatus and B. dorei, respectively. The patient was successfully treated with appropriate antibacterial therapy and after improvement, was transferred to another hospital for rehabilitation on postoperative day 34. There was no recurrence of infection or aneurysm after the patient transfer.

Conclusions

This report describes the first case of invasive infectious disease caused by B. dorei, casting a shadow over its utilisation as a probiotic for atherosclerotic diseases.