bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparative and Sex-Specific Study of Bio-Electrical Impedance Analysis and Dual Energy X-ray Absorptiometry for Estimating Whole-Body and Segmental Body Composition in Healthy Young Adults
Authors: D’Hondt Joachim , Waterplas Jana , Chapelle Laurent , Clarys Peter , D’Hondt Eva
Published: August 2022

Bio-electrical impedance analysis (BIA) and dual-energy X-ray absorptiometry (DXA) are methods to estimate human body composition. This study aimed to compare sex-specific outcomes for estimating segmental and whole-body composition in 83 healthy participants (21.9 ± 1.5 years, 56% men) using Inbody S10 BIA and Norland Elite DXA devices. One-way repeated measures ANOVAs showed significantly lower whole-body fat% and whole-body fat mass values alongside higher whole-body lean mass values resulting from BIA when compared to DXA (both sexes: p < 0.001). In men, whole-body bone mineral content was significantly higher using BIA against DXA (p < 0.001). Regardless of sex, no significant BIA versus DXA difference was found in arm fat mass (men: p = 0.180, women: p = 0.233), whereas significantly lower leg fat mass values were found with BIA versus DXA (both sexes: p < 0.001). Additionally, significantly higher arm lean mass (both sexes: p < 0.001) and leg lean mass (only women: p < 0.001) were found in BIA versus DXA. Moderate to very strong positive associations (p < 0.05) between BIA and DXA outcome measures were found, except for arm fat mass (men: p = 0.904, women: p = 0.130) and leg fat mass (only men: p = 0.845). This study highlights (sex-dependent) differences in corresponding test outcomes between BIA and DXA both at the segmental and whole-body level.
REX: General-Purpose CNL with Code Generation Support
Authors: Carvalho Adriano
Published: August 2022

Controlled natural languages (CNLs) have been proposed to address some of the issues of natural language when it is used to express requirements. CNLs, however, are based on formal grammar, which can easily become complex, hard to read, and especially hard to write, and the implementation of support tools can also demand a significant effort. Moreover, unanticipated constructions cannot be handled or have to be handled in unexpected and cumbersome ways. In this article, we present REX, a CNL with a simple grammar that is, thus, easy to understand and easy to support, but still general purpose. To accomplish this, instead of trying to support every conceivable construction and imposing a language on the users, through a small but comprehensive set of rules and through patterns, users specify their own language and how natural it is. Another of the benefits of CNLs is the possibility to automate the transformation of a text or specification into something useful, thereby reducing manual labor and transformation errors. In this article, we also present the support tools that were used to transform a REX text into code and a complete application. It is also shown that this CNL and its support tools can be easily adapted to suit different needs.
Musculoskeletal Symptoms and Assessment of Ergonomic Risk Factors on a Coffee Farm
Authors: Estrada-Muñoz Carla , Madrid-Casaca Héctor , Salazar-Sepúlveda Guido , Contreras-Barraza Nicolás , Iturra-González José , Vega-Muñoz Alejandro
Published: August 2022

In Honduras, some coffee farms must comply with strict standards of social, economic, and environmental sustainability, due to their organic, gender and fair-trade certifications. The principal research aim is to evaluate the musculoskeletal risks in occupations in a Honduran coffee farm certified in sustainable environments and to know the status of its workers within the farm. Musculoskeletal symptom perception during the last twelve months was consulted, assessing exposure to risk factors for work-related musculoskeletal disorders using the Quick Exposure Check method. Data regarding 48 workers were analyzed to provide the results. Within the body regions where discomfort is concentrated, the back, shoulders, wrists, knees, and feet stand out, and the highest risk exposures are presented for the coffee cutters at the neck level and in the wrist/hand segment, in the coffee pickers at the back, shoulder–arm segment, and wrist/hand segment, and in the processors in the back area and shoulder–arm segment. It is concluded that, in all the coffee fruit harvesting processes, the people who work in these jobs are exposed to ergonomic risks.
Development of a Convolutional Neural Network Model to Predict Coronary Artery Disease Based on Single-Lead and Twelve-Lead ECG Signals
Authors: Vasudeva Shrivathsa Thokur , Rao Shrikantha Sasihithlu , Panambur Navin Karanth , Shettigar Arun Kumar , Mahabala Chakrapani , Kamath Padmanabh , Gowdru Chandrashekarappa Manjunath Patel , Linul Emanoil
Published: August 2022

Coronary artery disease (CAD) is one of the most common causes of heart ailments; many patients with CAD do not exhibit initial symptoms. An electrocardiogram (ECG) is a diagnostic tool widely used to capture the abnormal activity of the heart and help with diagnoses. Assessing ECG signals may be challenging and time-consuming. Identifying abnormal ECG morphologies, especially in low amplitude curves, may be prone to error. Hence, a system that can automatically detect and assess the ECG and treadmill test ECG (TMT-ECG) signals will be helpful to the medical industry in detecting CAD. In the present work, we developed an intelligent system that can predict CAD, based on ECG and TMT signals more accurately than any other system developed thus far. The distinct convolutional neural network (CNN) architecture deals with single-lead and multi-lead (12-lead) ECG and TMT-ECG data effectively. While most artificial intelligence-based systems rely on the universal dataset, the current work used clinical lab data collected from a renowned hospital in the neighborhood. ECG and TMT-ECG graphs of normal and CAD patients were collected in the form of scanned reports. One-dimensional ECG data with all possible features were extracted from the scanned report with the help of a modified image processing method. This feature extraction procedure was integrated with the optimized architecture of the CNN model leading to a novel prediction system for CAD. The automated computer-assisted system helps in the detection and medication of CAD with a high prediction accuracy of 99%.
Temperature Sensing with Thin Films of Flame-Formed Carbon Nanoparticles
Authors: Minutolo Patrizia , De Falco Gianluigi , Commodo Mario , Aloisio Alberto , D’Anna Andrea
Published: August 2022

A porous nanostructured film of flame-formed carbon nanoparticles has been produced with a one-step procedure. The morphological and structural characteristics of the film have been characterized by atomic force microscopy and Raman spectroscopy. The electrical resistance as a function of the temperature has been investigated in the range from ambient temperature to 120 °C. A nonmetallic behavior has been observed, with a monotonic decrease of the film resistance as temperature increases. Electrical conduction is explained in terms of charge carriers tunneling and percolation between the carbon grains and is not perfectly described by an Arrhenius behavior. A negative temperature coefficient of resistance (TCR) of the order of −100 × 10−4 K−1 has been measured. The high absolute TCR value, together with the ease of material microfabrication processing and biocompatibility of the carbon material make this film ideal for temperature sensing in many environments. A functional relationship between resistance and temperature, which is necessary for practical applications, has been finally derived. A very good agreement between experimental data and fit is obtained with a fifth order polynomial.
Compressive Strength of Aged Timber Members in Traditional Building: Considering Differences in Heartwood and Sapwood
Authors: Yang Qingshan , Gao Chao , Liu Ke , Zhu Yingming , Wang Juan
Published: August 2022

This paper compares the parallel-to-grain compressive strengths of wood sample specimens with defects with those of the associated timber log samples. The Chinese fir (Cunninghamia lanceolata) was examined using the static test method. We analyzed the effects of sampling position along the radial direction of the log sample and the age of timber on the parallel-to-grain compressive strength. We then developed time-dependent strength models of the heartwood (HW) and sapwood (SW). These models can be used to evaluate the strength of an aged timber member, using only the service duration, without taking material samples. The degradation of compressive strength over time was then analyzed using an existing multivariate time-dependent model that includes the stress level. The failure strength of the HW was found to be close to that of the SW when at low stress levels. The estimates of the compressive strength of timber members based on these models were better than those recommended by the Chinese National Standard.
Assessment of Different Spent Mushroom Substrates to Bioremediate Soils Contaminated with Petroleum Hydrocarbons
Authors: Antón-Herrero Rafael , García-Delgado Carlos , Baena Natalia , Mayans Begoña , Delgado-Moreno Laura , Eymar Enrique
Published: August 2022

Bioremediation techniques are being developed as substitutes for physical–chemical methodologies that are expensive and not sustainable. For example, using the agricultural waste spent mushroom substrate (SMS) which contains valuable microbiota for soil bioremediation. In this work, SMSs of four cultivated fungal species, Pleurotus eryngii, Lentinula edodes, Pleurotus ostreatus, and Agaricus bisporus were evaluated for the bioremediation of soils contaminated by petroleum hydrocarbons (TPHs). The bioremediation test was carried out by mixing the four different SMSs with the TPH-contaminated soil in comparison with an unamended soil control to assess its natural attenuation. To determine the most efficient bioremediation strategy, hydrolase, dehydrogenase, and ligninolytic activities, ergosterol content, and percentage of TPHs degradation (total and by chains) were determined at the end of the assay at 40 days. The application of SMS significantly improved the degradation of TPHs with respect to the control. The most effective spent mushroom substrate to degrade TPHs was A. bisporus, followed by L. edodes and P. ostreatus. Similar results were obtained for the removal of aliphatic and aromatic hydrocarbons. The results showed the effectiveness of SMS to remove aliphatic and aromatic hydrocarbons from C10 to C35. This work demonstrates an alternative to valorizing an abundant agricultural waste as SMS to bioremediate contaminated soils.
Multiple-Image Reconstruction of a Fast Periodic Moving/State-Changed Object Based on Compressive Ghost Imaging
Authors: Guo Hui , Chen Yuxiang , Zhao Shengmei
Published: August 2022

We propose a multiple-image reconstruction scheme of a fast periodic moving/state-changed object with a slow bucket detector based on compressive ghost imaging, named MIPO-CSGI. To obtain N frames of an object with fast periodic moving/state-changed, N random speckle patterns are generated in each cycle of the object, which are then used to illuminate the object one by one. The total energy reflected from the object is recorded by a slow bucket detector at each cycle time T. Each group with N random speckle patterns is programmed as one row of a random matrix, and each row of the matrix element corresponds to one measurement of the slow bucket detector. Finally, the compressive sensing algorithm is applied to the constructed matrix and bucket detector signals, resulting in the direct acquisition of multiple images of the object. The feasibility of our method has been demonstrated in both numerical simulations and experiments. Hence, even with a slow bucket detector, MIPO-CSGI can image a fast periodic moving/state-changed object effectively.
Investigating the Difference of Fake News Source Credibility Recognition between ANN and BERT Algorithms in Artificial Intelligence
Authors: Chiang Tosti H. C. , Liao Chih-Shan , Wang Wei-Ching
Published: August 2022

Fake news permeating life through channels misleads people into disinformation. To reduce the harm of fake news and provide multiple and effective news credibility channels, the approach of linguistics is applied to a word-frequency-based ANN system and semantics-based BERT system in this study, using mainstream news as a general news dataset and content farms as a fake news dataset for the models judging news source credibility and comparing the difference in news source credibility recognition between ANN and BERT. The research findings show high similarity in the highest and lowest hit rates between the ANN system and the BERT system (Liberty Time had the highest hit rate, while ETtoday and nooho.net had the lowest hit rates). The BERT system presents a higher and more stable overall source credibility recognition rate than the ANN system (BERT 91.2% > ANN 82.75%). Recognizing news source credibility through artificial intelligence not only could effectively enhance people’s sensitivity to news sources but, in the long term, could cultivate public media literacy to achieve the synergy of fake news resistance with technology.
Spherical Tree-Structured SOM and Its Application to Hierarchical Clustering
Authors: Yoshioka Koki , Dozono Hiroshi
Published: August 2022

When analyzing high-dimensional data with many elements, a data visualization that maps the data onto a low-dimensional space is often performed. By visualizing the data, humans can intuitively understand the structure of the data in the high-dimensional space. The self-organizing map (SOM) is one such data visualization method. We propose a spherical tree-structured SOM (S-TS-SOM), which speeds up the search for winner nodes and eliminates the unevenness of learning due to the position of the winner nodes by placing the nodes on a sphere and applying the tree search method. In this paper, we confirm that the S-TS-SOM can achieve the same results as a normal spherical SOM while reducing the learning time. In addition, we confirm the granularity of clustering on the tree structure of the S-TS-SOM.
Variations in Sulfur and Nitrogen Oxidation Rates in Summer Aerosols from 2014 to 2020 in Wuhan, China
Authors: Zhao Jinhui , Ma Chiyuan , He Chao , Zhang Zhouxiang , Jiang Taotao , Tang Rui , Chen Qiang
Published: August 2022

To date, research regarding the changes of the sulfur and nitrogen rates in Wuhan during the summer is limited. In this study, we analyzed the air quality in Wuhan, China, using water-soluble ion, gaseous precursor, and weather data. A Spearman correlation analysis was then performed to investigate the temporal changes in air quality characteristics and their driving factors to provide a reference for air pollution control in Wuhan. The results indicate that SO2 in the atmosphere at Wuhan undergoes secondary conversion and photo-oxidation, and the conversion degree of SO2 is higher than that of NO2. During the summers of 2016 and 2017, secondary inorganic atmospheric pollution was more severe than during other years. The fewest oxidation days occurred in summer 2020 (11 days), followed by the summers of 2017 and 2014 (25 and 27 days, respectively). During the study period, ion neutralization was the strongest in summer 2015 and the weakest in August 2020. The aerosols in Wuhan were mostly acidic and NH4+ was an important neutralizing component. The neutralization factors of all cations showed little change in 2015. K+, Mg2+, and Ca2+ level changes were the highest in 2017 and 2020. At low temperature, high humidity, and low wind speed conditions, SO2 and NO2 were more easily converted into SO42− and NO3−.
Heavy Metal Accumulation and Phytoremediation Potentiality of Some Selected Mangrove Species from the World’s Largest Mangrove Forest
Authors: Hossain M. Belal , Masum Zobaer , Rahman M. Safiur , Yu Jimmy , Noman Md. Abu , Jolly Yeasmin N. , Begum Bilkis A. , Paray Bilal Ahamad , Arai Takaomi
Published: August 2022

Toxic metal pollution is a global issue, and the use of metal-accumulating plants to clean contaminated ecosystems is one of the most rapidly growing ecologically beneficial and cost-effective technologies. In this study, samples of sediment and three mangrove species (Excoecaria agallocha, Avicennia officinalis, Sonneratia apetala) were collected from the world’s largest mangrove forest (along the Northern Bay of Bengal Coast) with the aim of evaluating metal concentrations, contamination degrees, and phytoremediation potentiality of those plants. Overall, the heavy metals concentration in sediment ranged from Cu: 72.41–95.89 mg/kg; Zn: 51.28–71.20 mg/kg; Fe: 22,760–27,470 mg/kg; Mn: 80.37–116.37 mg/kg; Sr: 167.92–221.44 mg/kg. In mangrove plants, the mean concentrations were in the order of E. agallocha > A. officinalis > S. apetala. The mean (± SD) concentration of each metal in the plant tissue (root) was found following the descending order of Fe (737.37 ± 153.06) > Mn (151.13 ± 34.26) > Sr (20.98 ± 6.97) > Cu (16.12 ± 4.34) > Zn (11.3 ± 2.39) mg/kg, whereas, in the leaf part, the mean concentration (mg/kg) of each metal found in the order of Fe (598.75 ± 410.65) > Mn (297.27 ± 148.11) > Sr (21.40 ± 8.71) > Cu (14.25 ± 2.51) > Zn (12.56 ± 2.13). The contamination factor (CF) values for the studied metals were in the descending order of Cu > Sr > Zn > Fe > Mn. The values of Igeo (Geo-accumulation index) and CF showed that the area was unpolluted to moderately polluted by Zn, Fe, Mn, Cu and Sr. Enrichment factor (EF) values in both sampling stations portrayed moderate to minimum enrichment. Phytoremediation potentiality of the species was assessed by bio-concentration factor (BCF) and translocation factor (TF). BCF values showed less accumulation for most of the heavy metals (<1) except Mn which was highly accumulated in all mangrove plants. The translocation factor (TF) values depicted that most of the heavy metals were strongly accumulated in plant tissues (>1). However, the BCF value depicts that Mn was highly bioconcentrated in E. agallocha, but the translocation on leaves tissue were minimum, which reveals that E. agallocha is phytoextractor for Mn, and accumulated in root tissues. All the examined plants can be used as phytoextractors as they have bioconcentration factors <1 and translocation factors >1. However, A. officinalis is clearly more suitable for metal extraction than S. apetala and E. agallocha in terms of hyper-metabolizing capabilities.
Microalgae-Based Biorefineries: Challenges and Future Trends to Produce Carbohydrate Enriched Biomass, High-Added Value Products and Bioactive Compounds
Authors: Olguín Eugenia J. , Sánchez-Galván Gloria , Arias-Olguín Imilla I. , Melo Francisco J. , González-Portela Ricardo E. , Cruz Lourdes , De Philippis Roberto , Adessi Alessandra
Published: August 2022

Microalgae have demonstrated a large potential in biotechnology as a source of various macromolecules (proteins, carbohydrates, and lipids) and high-added value products (pigments, poly-unsaturated fatty acids, peptides, exo-polysaccharides, etc.). The production of biomass at a large scale becomes more economically feasible when it is part of a biorefinery designed within the circular economy concept. Thus, the aim of this critical review is to highlight and discuss challenges and future trends related to the multi-product microalgae-based biorefineries, including both phototrophic and mixotrophic cultures treating wastewater and the recovery of biomass as a source of valuable macromolecules and high-added and low-value products (biofertilizers and biostimulants). The therapeutic properties of some microalgae-bioactive compounds are also discussed. Novel trends such as the screening of species for antimicrobial compounds, the production of bioplastics using wastewater, the circular economy strategy, and the need for more Life Cycle Assessment studies (LCA) are suggested as some of the future research lines.
Differential Proteomic Analysis of Listeria monocytogenes during High-Pressure Processing
Authors: Chen Yi-An , Chen Guan-Wen , Ku Hao-Hsiang , Huang Tsui-Chin , Chang Hsin-Yi , Wei Cheng-I , Tsai Yung-Hsiang , Chen Tai-Yuan
Published: August 2022

High-pressure processing (HPP) is a prevailing non-thermal food preservation technology. The inactivation mechanisms of Listeria monocytogenes under HPP at 200 and 400 MPa for 3 min were investigated by label-free quantitative proteomic analysis and functional enrichment analysis in the Kyoto Encyclopedia of Genes and Genomes. HPP treatment at 400 MPa exhibited significant effects on proteins involved in translation, carbon, carbohydrate, lipid and energy metabolism, and peptidoglycan biosynthesis. HPP increased most ribosomal subunits and initiation factors, suggesting it might shift ribosomal biogenesis to translation initiation. However, protein synthesis was impaired by the shortage of proteins responsible for elongation, termination and recycling. HPP stimulated several ATP-dependent Clp proteases, and the global transcriptional regulator Spx, associating with activation of the stress-activated sigma factor Sigma B (σB) and the transcriptional activator positive regulatory factor A (PrfA) regulons. The quantitative proteomics approaches provide fundamental information on L. monocytogenes under different HPP pressures, and provide theoretical support for HPP against Listeriosis illness and for promotion of safer ready-to-eat foods.
Targeting the YXXΦ Motifs of the SARS Coronaviruses 1 and 2 ORF3a Peptides by In Silico Analysis to Predict Novel Virus—Host Interactions
Authors: Kakkanas Athanassios , Karamichali Eirini , Koufogeorgou Efthymia Ioanna , Kotsakis Stathis D. , Georgopoulou Urania , Foka Pelagia
Published: August 2022

The emerging SARS-CoV and SARS-CoV-2 belong to the family of “common cold” RNA coronaviruses, and they are responsible for the 2003 epidemic and the current pandemic with over 6.3 M deaths worldwide. The ORF3a gene is conserved in both viruses and codes for the accessory protein ORF3a, with unclear functions, possibly related to viral virulence and pathogenesis. The tyrosine-based YXXΦ motif (Φ: bulky hydrophobic residue—L/I/M/V/F) was originally discovered to mediate clathrin-dependent endocytosis of membrane-spanning proteins. Many viruses employ the YXXΦ motif to achieve efficient receptor-guided internalisation in host cells, maintain the structural integrity of their capsids and enhance viral replication. Importantly, this motif has been recently identified on the ORF3a proteins of SARS-CoV and SARS-CoV-2. Given that the ORF3a aa sequence is not fully conserved between the two SARS viruses, we aimed to map in silico structural differences and putative sequence-driven alterations of regulatory elements within and adjacently to the YXXΦ motifs that could predict variations in ORF3a functions. Using robust bioinformatics tools, we investigated the presence of relevant post-translational modifications and the YXXΦ motif involvement in protein-protein interactions. Our study suggests that the predicted YXXΦ-related features may confer specific—yet to be discovered—functions to ORF3a proteins, significant to the new virus and related to enhanced propagation, host immune regulation and virulence.
Viral Complexity
Authors: Aylward Frank O. , Moniruzzaman Mohammad
Published: August 2022

Although traditionally viewed as streamlined and simple, discoveries over the last century have revealed that viruses can exhibit surprisingly complex physical structures, genomic organization, ecological interactions, and evolutionary histories. Viruses can have physical dimensions and genome lengths that exceed many cellular lineages, and their infection strategies can involve a remarkable level of physiological remodeling of their host cells. Virus–virus communication and widespread forms of hyperparasitism have been shown to be common in the virosphere, demonstrating that dynamic ecological interactions often shape their success. And the evolutionary histories of viruses are often fraught with complexities, with chimeric genomes including genes derived from numerous distinct sources or evolved de novo. Here we will discuss many aspects of this viral complexity, with particular emphasis on large DNA viruses, and provide an outlook for future research.
The Rabep1-Mediated Endocytosis and Activation of Trypsinogen to Promote Pancreatic Stellate Cell Activation
Authors: Yao Wenchao , Luo Dankun , Lv Zhenyi , Yang Yang , Wang Liyi , Ma Biao , Xue Dongbo , Hao Chenjun , Zhang Yingmei
Published: August 2022

Background: The pathogenesis of chronic pancreatitis is still unclear. Trypsinogen activation is an active factor in acute pancreatitis that has not been studied in the occurrence of chronic pancreatitis. Methods: Immunofluorescence was used to detect the location and expression of trypsinogen in chronic pancreatitis and normal tissues. Microarray and single-cell RNA-seq (scRNA-seq) were used to screen core genes and pathways in pancreatic stellate cells (PSCs). Western blotting and immunofluorescence were used to verify trypsinogen expression in PSCs after silencing Rabep1. Immunofluorescence and flow cytometry were used to validate trypsinogen activation and PSC activation after intervening in the endocytosis pathway. Results: Endocytosed trypsinogen was found in PSCs in CP clinical samples. Bioinformatic analysis showed that Rabep1 is a core gene that regulates trypsinogen endocytosis through the endocytosis pathway, verified by Western blot and immunofluorescence. Immunofluorescence and flow cytometry analyses confirmed the activation of trypsinogen and PSCs through the endocytosis pathway in PSCs. Conclusion: This study discovered a new mechanism by which trypsinogen affects the activation of PSCs and the occurrence and development of CP. Through communication between pancreatic acinar cells and PSCs, trypsinogen can be endocytosed by PSCs and activated by the Rabep1 gene.
Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions
Authors: Ionescu Rodica Elena
Published: August 2022

Currently, several biosensors are reported to confirm the absence/presence of an abnormal level of specific human biomarkers in research laboratories. Unfortunately, public marketing and/or pharmacy accessibility are not yet possible for many bodily fluid biomarkers. The questions are numerous, starting from the preparation of the substrates, the wet/dry form of recognizing the (bio)ligands, the exposure time, and the choice of the running buffers. In this context, for the first time, the present overview summarizes the pre-functionalization of standard and nanostructured solid/flexible supports with cysteamine (Cys) and glutaraldehyde (GA) chemicals for robust protein immobilization and detection of biomarkers in body fluids (serum, saliva, and urine) using three transductions: piezoelectrical, electrochemical, and optical, respectively. Thus, the reader can easily access and compare step-by-step conjugate protocols published over the past 10 years. In conclusion, Cys/GA chemistry seems widely used for electrochemical sensing applications with different types of recorded signals, either current, potential, or impedance. On the other hand, piezoelectric detection via quartz crystal microbalance (QCM) and optical detection by surface plasmon resonance (LSPR)/surface-enhanced Raman spectroscopy (SERS) are ultrasensitive platforms and very good candidates for the miniaturization of medical devices in the near future.
Fabrication and Characterization of Acute Myocardial Infarction Myoglobin Biomarker Based on Chromium-Doped Zinc Oxide Nanoparticles
Authors: Al Fatease Adel , Haque Mazharul , Umar Ahmad , Ansari Shafeeque G. , Mahnashi Mater H. , Alhamhoom Yahya , Ansari Zubaida A.
Published: August 2022

In this article, we describe the fabrication and characterization of a sensor for acute myocardial infarction that detects myoglobin biomarkers using chromium (Cr)-doped zinc oxide (ZnO) nanoparticles (NPs). Pure and Cr-doped ZnO NPs (13 × 1017, 20 × 1017, and 32 × 1017 atoms/cm3 in the solid phase) were synthesized by a facile low-temperature sol-gel method. Synthesized NPs were examined for structure and morphological analysis using various techniques to confirm the successful formation of ZnO NPs. Zeta potential was measured in LB media at a negative value and increased with doping. XPS spectra confirmed the presence of oxygen deficiency in the synthesized material. To fabricate the sensor, synthesized NPs were screen-printed over a pre-fabricated gold-coated working electrode for electrochemical detection of myoglobin (Mb). Cr-doped ZnO NPs doped with 13 × 1017 Cr atomic/cm3 revealed the highest sensitivity of ~37.97 μA.cm−2nM−1 and limit of detection (LOD) of 0.15 nM for Mb with a response time of ≤10 ms. The interference study was carried out with cytochrome c (Cyt-c) due to its resemblance with Mb and human serum albumin (HSA) abundance in the blood and displayed distinct oxidation potential and current values for Mb. Cr-doped ZnO NP-based Mb biosensors showed 3 times higher sensitivity as compared to pure ZnO NP-based sensors.
Corpus Callosotomy: Editorial
Authors: Fujimoto Ayataka , Okanishi Tohru
Published: August 2022

Since corpus callosotomy (CC) was first reported in 1940 [...]
Effect of Scalp Nerve Block Combined with Intercostal Nerve Block on the Quality of Recovery in Patients with Parkinson’s Disease after Deep Brain Stimulation: Protocol for a Randomized Controlled Trial
Authors: Sheng Ying , Wang Hui , Chang Xinning , Jin Peipei , Lin Shengwei , Qian Shuang , Xie Jian , Lu Wenbin , Yu Xiya
Published: August 2022

Background: Parkinson’s disease (PD) patients who receive deep brain stimulation (DBS) have a higher risk of postoperative pain, which will affect their postoperative quality of recovery (QoR). Scalp nerve block (SNB) and intercostal nerve block (ICNB) can alleviate postoperative pain, yet their effect on postoperative QoR in PD patients has proven to be unclear. Therefore, we have aimed to explore the effect of SNB paired with ICNB on postoperative QoR. Methods: To explore the effect, we have designed a randomized controlled trial in which 88 patients with PD will be randomly assigned to either an SNB group or control group, receiving either SNB combined with ICNB or without before surgery. The primary outcome will be a 15-item QoR score at 24 h after surgery. The secondary outcomes will include: 15-item QoR scores at 72 h and 1 month after surgery; the numeric rating scale pain scores before discharge from the postanesthesia care unit (PACU) at 24 h, 72 h, and 1 month after surgery; rescue analgesics; nausea and vomiting 24 h after operation and remifentanil consumption during operation; emergence agitation; the duration of anesthesia and surgery; time to respiratory recovery, time to response, and time to extubation; the PACU length of stay; as well as adverse events. Proposed protocol and conclusion: Our findings will provide a novel method for the management of recovery and acute pain after DBS in PD patients. This research was registered at clinicaltrials.gov NCT05353764 on 19 April 2022.
The Reliability and Validity of Speech-Language Pathologists’ Estimations of Intelligibility in Dysarthria
Authors: Hirsch Micah E. , Thompson Austin , Kim Yunjung , Lansford Kaitlin L.
Published: August 2022

This study examined the reliability and validity of speech-language pathologists’ (SLP) estimations of speech intelligibility in dysarthria, including a visual analog scale (VAS) method and a percent estimation method commonly used in clinical settings. Speech samples from 20 speakers with dysarthria of varying etiologies were used to collect orthographic transcriptions from naïve listeners n=70 and VAS ratings and percent estimations of intelligibility from SLPs n=21. Intra- and interrater reliability for the two SLP intelligibility measures were evaluated, and the relationship between these measures was assessed. Finally, linear regression was used to evaluate the relationship between the naïve listeners’ orthographic transcription scores and the two SLP intelligibility measures. The results indicated that the intrarater reliability for both SLP intelligibility measures was strong, and the interrater reliability between the SLP ratings was moderate to excellent. A moderate positive relationship between SLPs’ VAS ratings and percent estimations was also observed. Finally, both SLPs’ percent estimations and VAS ratings were predictive of naïve listeners’ orthographic transcription scores, with SLPs’ percent estimations being the strongest predictor. In conclusion, the average SLP percent estimations and VAS ratings are valid and reliable intelligibility measures. However, the validity and reliability of these measures vary between SLPs.
Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma
Authors: Zhang Yi-Bin , Zheng Shu-Fa , Ma Lin-Jie , Lin Peng , Shang-Guan Huang-Cheng , Lin Yuan-Xiang , Kang De-Zhi , Yao Pei-Sen
Published: August 2022

Objective Glioblastoma (GBM), a type of malignant glioma, is the most aggressive type of brain tumor and is associated with high mortality. Hexose-6-phosphate dehydrogenase (H6PD) has been detected in multiple tumors and is involved in tumor initiation and progression. However, the specific role and mechanism of H6PD in GBM remain unclear. Methods We performed pan-cancer analysis of expression and prognosis of H6PD in GBM using the Genotype-Tissue Expression Project (GTEx) and The Cancer Genome Atlas (TCGA). Subsequently, noncoding RNAs regulating H6PD expression were obtained by comprehensive analysis, including gene expression, prognosis, correlation, and immune infiltration. Finally, tumor immune infiltrates related to H6PD and survival were performed. Results Higher expression of H6PD was statistically significantly associated with an unfavorable outcome in GBM. Downregulation of hsa-miR-124-3p and hsa-miR-516b-5p in GBM was detected from GSE90603. Subsequently, OSMR-AS1 was observed in the regulation of H6PD via hsa-miR-516b-5p. Moreover, higher H6PD expression significantly correlated with immune infiltration of dendritic cells, immune checkpoint expression, and biomarkers of dendritic cells. Conclusions The OSMR-AS1/ miR-516b-5p axis was identified as the highest-potential upstream ncRNA-related pathway of H6PD in GBM. Furthermore, the present findings demonstrated that H6PD blockading might possess antitumor roles via regulating dendritic cell infiltration and immune checkpoint expression.
Altered Development of Prefrontal GABAergic Functions and Anxiety-like Behavior in Adolescent Offspring Induced by Prenatal Stress
Authors: Suwaluk Arbthip , Chutabhakdikul Nuanchan
Published: August 2022

Maternal stress can afflict fetal brain development, putting the offspring at risk of cognitive deficits, including anxiety. The prefrontal cortex (PFC), a protracted maturing region, is notably affected by prenatal stress (PS). However, it remains unclear how PS interferes with the maturation of the GABAergic system, considering its functional adjustment in the PFC during adolescence. The present study thus investigated the long-lasting consequences of PS on the prefrontal GABAergic functions of adolescent offspring. Pregnant Sprague–Dawley rats were divided into controls and the PS group, which underwent restraint stress during the last week of gestation. Male pups from postnatal days (PND) 40–42 were submitted to the elevated plus maze (EPM) test. Proteins essentially involved in GABAergic signaling were then examined in PFC tissues, including the K+-Cl− cotransporter (KCC2), Na+-K+-Cl− cotransporter (NKCC1), α1 and α5 subunits of GABA type A receptors (GABAA receptors), and parvalbumin (PV), along with cAMP response element-binding protein phosphorylation (pCREB), which reacts in the plasticity regulation of PV-positive interneurons. The results revealed that the higher anxiety-like behavior of PS adolescent rats concurred with the significant decreases of the KCC2 and α1 subunits, with PV- and pCREB-lowered levels. The findings suggested that PS disrupts the continuance of PFC maturity by reducing the essential elements of GABAergic functions. These changes likely underlie the anxiety emerging in adolescence, possibly progressing to mental disorders.
Nonlinear Seismic Assessment of a Historic Rubble Masonry Building via Simplified and Advanced Computational Approaches
Authors: Hamp Elyse , Gerber Rachel , Pulatsu Bora , Quintero Mario , Erochko Jeffrey
Published: August 2022

This research presents a comprehensive nonlinear quasi-static seismic assessment of an unreinforced rubble masonry building, Bytown Museum in Ottawa, Canada, using discontinuum-based analyses. In the proposed modeling approach, non-uniform geometrical properties of rubble masonry walls are replicated via a group of rigid polyhedral blocks interacting along their boundaries based on the discrete element method (DEM). Once the adopted modeling strategy is validated, the nonlinear quasi-static analysis of the South and North façades of the Bytown Museum is performed. Special attention is given to the irregular block generation within the discontinuum analysis framework, where discrete element models are generated from high-resolution site recording data, representing the masonry morphology at a high level of detail. Then, the predicted collapse mechanisms from advanced computational models are further utilized to generate pre-defined macro-blocks in kinematic limit analyses, providing a simple alternative solution for seismic assessment. The results reveal the significant effect of openings and the construction technique (morphology) in unreinforced rubble masonry buildings that can play an important role in the structural capacity and behavior. Moreover, it is noted that DEM-based solutions provide lower seismic capacity compared to kinematic limit analyses. Finally, a noticeable sensitivity to the input parameters in the discrete element models is noted; therefore, characterization of material properties is necessary for reliable predictions.
Intra-Operative Electron Radiation Therapy: An Update of the Evidence Collected in 40 Years to Search for Models for Electron-FLASH Studies
Authors: Calvo Felipe A. , Serrano Javier , Cambeiro Mauricio , Aristu Javier , Asencio Jose Manuel , Rubio Isabel , Delgado Jose Miguel , Ferrer Carlos , Desco Manuel , Pascau Javier
Published: August 2022

Introduction: The clinical practice and outcome results of intraoperative electron radiation therapy (IOeRT) in cancer patients have been extensively reported over 4 decades. Electron beams can be delivered in the promising FLASH dose rate. Methods and Materials: Several cancer models were approached by two alternative radiobiological strategies to optimize local cancer control: boost versus exclusive IOeRT. Clinical outcomes are revisited via a bibliometric search performed for the elaboration of ESTRO/ACROP IORT guidelines. Results: In the period 1982 to 2020, a total of 19,148 patients were registered in 116 publications concerning soft tissue sarcomas (9% of patients), unresected and borderline-resected pancreatic cancer (22%), locally recurrent and locally advanced rectal cancer (22%), and breast cancer (45%). Clinical outcomes following IOeRT doses in the range of 10 to 25 Gy (with or without external beam fractionated radiation therapy) show a wide range of local control from 40 to 100% depending upon cancer site, histology, stage, and treatment intensity. Constraints for normal tissue tolerance are important to maintain tumor control combined with acceptable levels of side effects. Conclusions: IOeRT represents an evidence-based approach for several tumor types. A specific risk analysis for local recurrences supports the identification of cancer models that are candidates for FLASH studies.
Adding Concomitant Chemotherapy to Postoperative Radiotherapy in Oral Cavity Carcinoma with Minor Risk Factors: Systematic Review of the Literature and Meta-Analysis
Authors: Di Rito Alessia , Fiorica Francesco , Carbonara Roberta , Di Pressa Francesca , Bertolini Federica , Mannavola Francesco , Lohr Frank , Sardaro Angela , D’Angelo Elisa
Published: August 2022

When presenting with major pathological risk factors, adjuvant radio-chemotherapy for oral cavity cancers (OCC) is recommended, but the addition of chemotherapy to radiotherapy (POCRT) when only minor pathological risk factors are present is controversial. A systematic review following the PICO-PRISMA methodology (PROSPERO registration ID: CRD42021267498) was conducted using the PubMed, Embase, and Cochrane libraries. Studies assessing outcomes of POCRT in patients with solely minor risk factors (perineural invasion or lymph vascular invasion; pN1 single; DOI ≥ 5 mm; close margin < 2–5 mm; node-positive level IV or V; pT3 or pT4; multiple lymph nodes without ENE) were evaluated. A meta-analysis technique with a single-arm study was performed. Radiotherapy was combined with chemotherapy in all studies. One study only included patients treated with POCRT. In the other 12 studies, patients were treated with only PORT (12,883 patients) and with POCRT (10,663 patients). Among the patients treated with POCRT, the pooled 3 year OS rate was 72.9% (95%CI: 65.5–79.2%); the pooled 3 year DFS was 70.9% (95%CI: 48.8–86.2%); and the pooled LRFS was 69.8% (95%CI: 46.1–86.1%). Results are in favor of POCRT in terms of OS but not significant for DFS and LRFS, probably due to the heterogeneity of the included studies and a combination of different prognostic factors.
Drug Repurposing, a Fast-Track Approach to Develop Effective Treatments for Glioblastoma
Authors: Ntafoulis Ioannis , Koolen Stijn L. W. , Leenstra Sieger , Lamfers Martine L. M.
Published: August 2022

Glioblastoma (GBM) remains one of the most difficult tumors to treat. The mean overall survival rate of 15 months and the 5-year survival rate of 5% have not significantly changed for almost 2 decades. Despite progress in understanding the pathophysiology of the disease, no new effective treatments to combine with radiation therapy after surgical tumor debulking have become available since the introduction of temozolomide in 1999. One of the main reasons for this is the scarcity of compounds that cross the blood–brain barrier (BBB) and reach the brain tumor tissue in therapeutically effective concentrations. In this review, we focus on the role of the BBB and its importance in developing brain tumor treatments. Moreover, we discuss drug repurposing, a drug discovery approach to identify potential effective candidates with optimal pharmacokinetic profiles for central nervous system (CNS) penetration and that allows rapid implementation in clinical trials. Additionally, we provide an overview of repurposed candidate drug currently being investigated in GBM at the preclinical and clinical levels. Finally, we highlight the importance of phase 0 trials to confirm tumor drug exposure and we discuss emerging drug delivery technologies as an alternative route to maximize therapeutic efficacy of repurposed candidate drug.
Association between Vitamin D Supplementation and Cancer Mortality: A Systematic Review and Meta-Analysis
Authors: Zhang Renjie , Zhang Yu , Liu Zheran , Pei Yiyan , Xu Ping , Chong Weelic , Hai Yang , He Ling , He Yan , Yu Jiayi , Wang Jingjing , Fang Fang , Peng Xingchen
Published: August 2022

Background: Vitamin D deficiency is related to increased cancer risk and deaths. However, whether vitamin D supplementation reduces cancer mortality remains unclear, and several randomized controlled trials yield inconsistent results. Methods: Medline, Embase, and the Cochrane Central Register of Controlled Trials were searched from their inception until 28 June 2022, for randomized controlled trials investigating vitamin D supplementation. Pooled relative risks (RRs) and their 95% confidence intervals (CIs) were estimated. Trials with vitamin D supplementation combined with calcium supplementation versus placebo alone and recruiting participants with cancer at baseline were excluded in the present study. Results: This study included 12 trials with a total of 72,669 participants. Vitamin D supplementation did not reduce overall cancer mortality (RR 0.96, 95% CI 0.80–1.16). However, vitamin D supplementation was associated with a reduction in lung cancer mortality (RR 0.63, 95% CI 0.45–0.90). Conclusions: Vitamin D supplementation could not reduce cancer mortality in this highly purified meta-analysis. Further RCTs that evaluate the association between vitamin D supplementation and total cancer mortality are still needed.
The Thousand Faces of Malignant Melanoma: A Systematic Review of the Primary Malignant Melanoma of the Esophagus
Authors: Cazzato Gerardo , Cascardi Eliano , Colagrande Anna , Lettini Teresa , Resta Leonardo , Bizzoca Cinzia , Arezzo Francesca , Loizzi Vera , Dellino Miriam , Cormio Gennaro , Casatta Nadia , Lupo Carmelo , Scillimati Antonio , Scacco Salvatore , Parente Paola , Lospalluti Lucia , Ingravallo Giuseppe
Published: August 2022

Primary Malignant Melanoma of the Esophagus (PMME) is an extremely rare cancer of the esophagus, accounting for 0.1–0.8% of all oro-esophageal cancers and <0.05% of all melanoma subtypes, with an estimated incidence of 0.0036 cases per million/year. We conduct a careful analysis of the literature starting from 1906 to the beginning of 2022, searching the PubMed, Science.gov, Scopus and Web of Science (WoS) databases. A total of 457 records were initially identified in the literature search, of which 17 were duplicates. After screening for eligibility and inclusion criteria, 303 publications were ultimately included, related to 347 patients with PMME. PMME represents a very rare entity whose very existence has been the subject of debate for a long time. Over time, an increasing number of cases have been reported in the literature, leading to an increase in knowledge and laying the foundations for a discussion on the treatment of this pathology, which still remains largely represented by surgery. In recent times, the possibility of discovering greater mutations in gene hotspots has made it possible to develop new therapeutic strategies of which nivolumab is an example. Future studies with large case series, with clinicopathological and molecular data, will be necessary to improve the outcome of patients with PMME.