bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolutionary Optimization of Energy Consumption and Makespan of Workflow Execution in Clouds
Authors: Xing Lining , Li Jun , Cai Zhaoquan , Hou Feng
Published: May 2023

Making sound trade-offs between the energy consumption and the makespan of workflow execution in cloud platforms remains a significant but challenging issue. So far, some works balance workflows’ energy consumption and makespan by adopting multi-objective evolutionary algorithms, but they often regard this as a black-box problem, resulting in the low efficiency of the evolutionary search. To compensate for the shortcomings of existing works, this paper mathematically formulates the cloud workflow scheduling for an infrastructure-as-a-service (IaaS) platform as a multi-objective optimization problem. Then, this paper tailors a knowledge-driven energy- and makespan-aware workflow scheduling algorithm, namely EMWSA. Specifically, a critical task adjustment-based local search strategy is proposed to intelligently adjust some critical tasks to the same resource of their successor tasks, striving to simultaneously reduce workflows’ energy consumption and makespan. Further, an idle gap reuse strategy is proposed to search the optimal energy consumption of each non-critical task without affecting the operation of other tasks, so as to further reduce energy consumption. Finally, in the context of real-world workflows and cloud platforms, we carry out comparative experiments to verify the superiority of the proposed EMWSA by significantly outperforming 4 representative baselines on 19 out of 20 workflow instances.
Isolation of Nocuolin A and Synthesis of New Oxadiazine Derivatives. Design, Synthesis, Molecular Docking, Apoptotic Evaluation, and Cathepsin B Inhibition
Authors: Tena Pérez Víctor , Apaza Ticona Luis , H. Cabanillas Alfredo , Maderuelo Corral Santiago , Rosero Valencia Diego Fernando , Martel Quintana Antera , Ortega Domenech Montserrat , Rumbero Sánchez Ángel
Published: May 2023

Nocuolin A (1), an oxadiazine, was isolated from the cyanobacterium Nostoc sp. Its chemical structure was elucidated using NMR and mass spectroscopic data. From this compound, two new oxadiazines, 3-[(6R)-5,6-dihydro-4,6-dipentyl-2H-1,2,3-oxadiazin-2-yl]-3-oxopropyl acetate (2) and 4-{3-[(6R)-5,6-dihydro-4,6-dipentyl-2H-1,2,3-oxadiazin-2-yl]-3-oxopropoxy}-4-oxobutanoic acid (3), were synthesised. The chemical structures of these two compounds were elucidated by a combination of NMR and MS analysis. Compound 3 showed cytotoxicity against the ACHN (0.73 ± 0.10 μM) and Hepa-1c1c7 (0.91 ± 0.08 μM) tumour cell lines. Similarly, compound 3 significantly decreased cathepsin B activity in ACHN and Hepa-1c1c7 tumour cell lines at concentrations of 1.52 ± 0.13 nM and 1.76 ± 0.24 nM, respectively. In addition, compound 3 showed no in vivo toxicity in a murine model treated with a dose of 4 mg/kg body weight.
Structure and Performance of All-Green Electrospun PHB-Based Membrane Fibrous Biomaterials Modified with Hemin
Authors: Tyubaeva Polina , Varyan Ivetta , Krivandin Alexey , Shatalova Olga , Olkhov Anatoly , Popov Anatoly , Xu Huaizhong , Arzhakova Olga
Published: May 2023

This work addresses the challenges concerning the development of “all-green” high-performance biodegradable membrane materials based on poly-3-hydroxybutyrate (PHB) and a natural biocompatible functional additive, iron-containing porphyrin, Hemin (Hmi) via modification and surface functionalization. A new facile and versatile approach based on electrospinning (ES) is advanced when modification of the PHB membranes is performed by the addition of low concentrations of Hmi (from 1 to 5 wt.%). Structure and performance of the resultant {HB/Hmi membranes were studied by diverse physicochemical methods, including differential scanning calorimetry, X-ray analysis, scanning electron microscopy, etc. Modification of the PHB fibrous membranes with Hmi allows control over their quality, supramolecular structure, morphology, and surface wettability. As a result of this modification, air and liquid permeability of the modified electrospun materials markedly increases. The proposed approach provides preparation of high-performance all-green membranes with tailored structure and performance for diverse practical applications, including wound healing, comfort textiles, facial protective masks, tissue engineering, water and air purification, etc.
Engineering Metal-Organic-Framework (MOF)-Based Membranes for Gas and Liquid Separation
Authors: Duan Yutian , Li Lei , Shen Zhiqiang , Cheng Jian , He Kewu
Published: May 2023

Separation is one of the most energy-intensive processes in the chemical industry, and membrane-based separation technology contributes significantly to energy conservation and emission reduction. Additionally, metal-organic framework (MOF) materials have been widely investigated and have been found to have enormous potential in membrane separation due to their uniform pore size and high designability. Notably, pure MOF films and MOF mixed matrix membranes (MMMs) are the core of the “next generation” MOF materials. However, there are some tough issues with MOF-based membranes that affect separation performance. For pure MOF membranes, problems such as framework flexibility, defects, and grain orientation need to be addressed. Meanwhile, there still exist bottlenecks for MMMs such as MOF aggregation, plasticization and aging of the polymer matrix, poor interface compatibility, etc. Herein, corresponding methods are introduced to solve these problems, including inhibiting framework flexibility, regulating synthesis conditions, and enhancing the interaction between MOF and substrate. A series of high-quality MOF-based membranes have been obtained based on these techniques. Overall, these membranes revealed desired separation performance in both gas separation (e.g., CO2, H2, and olefin/paraffin) and liquid separation (e.g., water purification, organic solvent nanofiltration, and chiral separation).
Separation Performance of Membranes Containing Ultrathin Surface Coating of Metal-Polyphenol Network
Authors: Kinfu Hluf Hailu , Rahman Md. Mushfequr
Published: May 2023

Metal–polyphenol networks (MPNs) are being used as versatile coatings for regulating membrane surface chemistry and for the formation of thin separation layers. The intrinsic nature of plant polyphenols and their coordination with transition metal ions provide a green synthesis procedure of thin films, which enhance membrane hydrophilicity and fouling resistance. MPNs have been used to fabricate tailorable coating layers for high-performance membranes desirable for a wide range of applications. Here, we present the recent progress of the use of MPNs in membrane materials and processes with a special focus on the important roles of tannic acid–metal ion (TA-Mn+) coordination for thin film formation. This review introduces the most recent advances in the fabrication techniques and the application areas of TA-Mn+ containing membranes. In addition, this paper outlines the latest research progress of the TA–metal ion containing membranes and summarizes the role of MPNs in membrane performance. The impact of fabrication parameters, as well as the stability of the synthesized films, is discussed. Finally, the remaining challenges that the field still faces and potential future opportunities are illustrated.
ZnO/PDA/Mesoporous Cellular Foam Functionalized Thin-Film Nanocomposite Membrane towards Enhanced Nanofiltration Performance
Authors: Nambikkattu Jenny , Thomas Anoopa Ann , Kaleekkal Noel Jacob , Arumugham Thanigaivelan , Hasan Shadi W. , Vigneswaran Saravanamuthu
Published: May 2023

Thin-film nanocomposite (TFN) membranes are the third-generation membranes being explored for nanofiltration applications. Incorporating nanofillers in the dense selective polyamide (PA) layer improves the permeability–selectivity trade-off. The mesoporous cellular foam composite Zn-PDA-MCF-5 was used as a hydrophilic filler in this study to prepare TFN membranes. Incorporating the nanomaterial onto the TFN-2 membrane resulted in a decrease in the water contact angle and suppression of the membrane surface roughness. The pure water permeability of 6.40 LMH bar−1 at the optimal loading ratio of 0.25 wt.% obtained was higher than the TFN-0 (4.20 LMH bar−1). The optimal TFN-2 demonstrated a high rejection of small-sized organics (>95% rejection for 2,4-dichlorophenol over five cycles) and salts—Na2SO4 (≈95%) > MgCl2 (≈88%) > NaCl (86%) through size sieving and Donnan exclusion mechanisms. Furthermore, the flux recovery ratio for TFN-2 increased from 78.9 to 94.2% when challenged with a model protein foulant (bovine serum albumin), indicating improved anti-fouling abilities. Overall, these findings provided a concrete step forward in fabricating TFN membranes that are highly suitable for wastewater treatment and desalination applications.
A Strategical Improvement in the Performance of CO2/N2 Gas Permeation via Conjugation of L-Tyrosine onto Chitosan Membrane
Authors: Katare Aviti , Borgohain Rajashree , Prasad Babul , Mandal Bishnupada
Published: May 2023

Rubbery polymeric membranes, containing amine carriers, have received much attention in CO2 separation because of their easy fabrication, low cost, and excellent separation performance. The present study focuses on the versatile aspects of covalent conjugation of L-tyrosine (Tyr) onto the high molecular weight chitosan (CS) accomplished by using carbodiimide as a coupling agent for CO2/N2 separation. The fabricated membrane was subjected to FTIR, XRD, TGA, AFM, FESEM, and moisture retention tests to examine the thermal and physicochemical properties. The defect-free dense layer of tyrosine-conjugated-chitosan, with active layer thickness within the range of ~600 nm, was cast and employed for mixed gas (CO2/N2) separation study in the temperature range of 25−115 °C in both dry and swollen conditions and compared to that of a neat CS membrane. An enhancement in the thermal stability and amorphousness was displayed by TGA and XRD spectra, respectively, for the prepared membranes. The fabricated membrane showed reasonably good CO2 permeance of around 103 GPU and CO2/N2 selectivity of 32 by maintaining a sweep/feed moisture flow rate of 0.05/0.03 mL/min, respectively, an operating temperature of 85 °C, and a feed pressure of 32 psi. The composite membrane demonstrated high permeance because of the chemical grafting compared to the bare chitosan. Additionally, the excellent moisture retention capacity of the fabricated membrane accelerates high CO2 uptake by amine carriers, owing to the reversible zwitterion reaction. All the features make this membrane a potential membrane material for CO2 capture.
Measuring Physical Properties of Electrospun Nanofiber Mats for Different Biomedical Applications
Authors: Langwald Sarah Vanessa , Ehrmann Andrea , Sabantina Lilia
Published: May 2023

Electrospun nanofiber mats are nowadays often used for biotechnological and biomedical applications, such as wound healing or tissue engineering. While most studies concentrate on their chemical and biochemical properties, the physical properties are often measured without long explanations regarding the chosen methods. Here, we give an overview of typical measurements of topological features such as porosity, pore size, fiber diameter and orientation, hydrophobic/hydrophilic properties and water uptake, mechanical and electrical properties as well as water vapor and air permeability. Besides describing typically used methods with potential modifications, we suggest some low-cost methods as alternatives in cases where special equipment is not available.
Monoethanolamine (MEA) Degradation: Influence on the Electrodialysis Treatment of MEA-Absorbent
Authors: Novitsky Eduard G. , Grushevenko Evgenia A. , Borisov Ilya L. , Anokhina Tatiana S. , Bazhenov Stepan D.
Published: May 2023

The thermal-oxidative degradation of aqueous solutions of carbonized monoethanolamine (MEA, 30% wt., 0.25 mol MEA/mol CO2) was studied for 336 h at 120 °C. Based on the change in the color of the solution and the formation of a precipitate, the occurrence of thermal-oxidative degradation of the MEA solution with the formation of destruction products, including insoluble ones, was confirmed. The electrokinetic activity of the resulting degradation products, including insoluble ones, was studied during the electrodialysis purification of an aged MEA solution. To understand the influence of degradation products on the ion-exchange membrane properties, a package of samples of MK-40 and MA-41 ion-exchange membranes was exposed to a degraded MEA solution for 6 months. A comparison of the efficiency of the electrodialysis treatment of a model absorption solution of MEA before and after long-time contact with degraded MEA showed that the depth of desalination was reduced by 34%, while the magnitude of the current in the ED apparatus was reduced by 25%. For the first time, the regeneration of ion-exchange membranes from MEA degradation products was carried out, which made it possible to restore the depth of desalting in the ED process by 90%.
Mechanical Characterization at Nanoscale of Austenite, Ferrite, and Sigma Phases via Hardness Measurement and Fretting Wear Behavior of a Duplex Stainless Steel
Authors: Ribeiro Jomar José Knaip , Turin Alba Regina , Nuñez de la Rosa Yamid E. , Quadros Pedro Victorio Caetano Abrantes , Calabokis Oriana Palma , Lepienski Carlos Maurício , Brunatto Silvio Francisco , Borges Paulo César
Published: May 2023

This study aimed at the mechanical characterization, on a nanometric scale, of the constituents obtained for different fractions in duplex stainless-steel plates subjected to 850, 950, 1000, and 1150 °C heating treatments via hardness measurements and determining their influences on the fretting wear behavior of the studied steel. The obtained ferrite (α)-, austenite (γ)-, and sigma (σ)-phase fractions were determined using optical microscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD) techniques. The mechanical characterization was carried out using hardness measurement and fretting wear techniques via nanoindentation. For comparison purposes, the Vickers microhardness was also characterized to determine the effect of the σ phase, which eventually formed, on the obtained microstructure properties as a whole. Two distinct behaviors were observed, depending on the eventual formation of σ phase as a function of the treatment temperature: (i) specimens treated at 850 and 950 °C showed a hardening effect (HV0.5 values of 333 ± 15 and 264 ± 13, respectively) due to σ-phase precipitation (hereafter termed ‘as-aged’), and (ii) specimens treated at 1000 and 1150 °C (with HV0.5 values of 240 ± 13 and 249 ± 4, respectively) showed no σ-phase precipitation (hereafter termed ‘as-solubilized’). The increases in the microhardness values for the as-aged specimens were attributed to the hardness of the σ-phase precipitates (which showed nanohardness values varying in the 8.0–8.5 GPa range), which was approximately twice that of the austenite and ferrite grains (both phases showed nanohardness values in the 3.6–4.1 GPa range, on average). When formed (for fractions on the order of 8% and 3% at 850 and 950 °C, respectively), σ phase was mainly observed at the α/γ grain interfaces or boundaries. Fretting wear tests, using a diamond sphere with a radius of 10 μm as the counter body and a load of 20 mN, revealed the same wear mechanisms in the α/γ matrix for all studied conditions. However, as-solubilized specimens (heat-treated at 1000 and 1150 °C) displayed higher resistance to fretting micro-wear in the austenitic grains compared to the ferritic grains, indicating lower plastic deformation in the respective wear scars on the obtained tracks. In particular, as-aged specimens (heat-treated at 850 and 950 °C) exhibited lower coefficients of friction due to their higher surface resistances. The localized wear at σ-phase grains was much less pronounced than at ferrite and austenite grains. Overall, this study provides valuable insights into the mechanical behavior of microstructural changes in duplex steel at the nanometric scale.
Effects of Borax and Grinding Alkalinity on the Reduction–Magnetic Separation of Beach Placer
Authors: Hu Bing , Hu Peiwei , Gao Runqin , Hu Chao , Zheng Fuqiang
Published: May 2023

The effect of borax on the reduction characteristics of Indonesia beach placers was investigated. The effect of grinding alkalinity on the magnetic separation of the reduced sample was also studied in this paper. The mineral phase transformation, microstructures of reduction, and magnetic separation products were analyzed to reveal the enhanced separation mechanism of titanium and iron in beach placer. The borax could effectively improve the metallization rate and the growth of iron grains in a reduced sample. When 3% borax was added to the reduction process, the metallization rate of the reduced beach placer reached 95.64%, and the metal iron grains grew to about 50 μm. Adjusting the grinding alkalinity could prevent the metallic iron from being oxidized and promote the monomer dissociation between mineral particles. The iron powder concentrates with 94.07% total Fe and vanadium-rich titanium slag with 36.32% Ti were obtained by grinding magnetic separation as the grinding alkalinity pH was 13.
Regulation of Microstructure to Optimize Mechanical Properties of Ti-15Mo-3Al-2.7Nb-0.2Si via Solution-Duplex Ageing
Authors: Kang Xudong , Jiang Hanyu , Du Zhaoxin , Gong Tianhao , Liu Jingwen , Guo Wenxia , Cheng Jun , Liu Jingshun , Li Guowei
Published: May 2023

The production of alloys with high strength and toughness concurrently is still a difficult challenge. Here, we designed a simple solution-ageing heat treatment system to control the morphology and density of α in Ti-15Mo-3Al-2.7Nb-0.2Si via different heat treatment temperatures. The experimental results show that Ti-15Mo-3Al-2.7Nb-0.2Si exhibits a synergistic combination of tensile strength (1364 MPa), plasticity (7.8% elongation), and fracture toughness (101 MPa·m1/2) through solutions in the α/β biphasic region and duplex ageing. Notably, the strength of the alloy after the second step of the ageing process is increased by 15% compared with that after the first step of the ageing process. However, there is less than a 5% reduction in the fracture toughness. TEM observations show that the matrix continues to precipitate denser secondary α during duplex ageing, which causes the strength to increase significantly and causes the fracture toughness to weaken. Our work may provide a novel method to optimize the mechanical properties of alloys by controlling the precipitates.
Effect of Cryogenic Time on Microstructure and Properties of TRCed AZ31 Magnesium Alloy Sheets Rolled during Cryogenic Rolling
Authors: Zhi Chen-Chen , Li Pei-Jin , Huang Zhi-Quan , Liu Peng-Tao , Xu Hai-Jie , Jia Wei-Tao , Ma Li-Feng
Published: May 2023

Rolling experiments of TRCed AZ31 magnesium alloy with different cryogenic treatment time were carried out to study the evolution mechanism of its microstructure and mechanical properties. The experimental results showed that with the increase in cryogenic time, the grain size of the sheets after cryogenic rolling was significantly refined, and the dislocation density and texture strength were greatly weakened. The combined effect led to a significant increase in the elongation and tensile strength of the sheet after cryogenic rolling. The tensile strength, elongation and average hardness of the sheet increased from 282.6 MPa, 8.2%, and 54.6 HV to 305.4 MPa, 16.3%, and 62.8 HV, respectively. Therefore, when the cryogenic treatment time was 60 s, the performance of the rolled sheet was the best. At the same time, the appearance of dimples after cryogenic rolling led to a change of the fracture mechanism, which was also the key to the improvement of the sheet elongation.
Green Approach for Electropolishing Surface Treatments of Additive Manufactured Parts: A Comprehensive Review
Authors: Acquesta Annalisa , Monetta Tullio
Published: May 2023

Over the years, the widespread diffusion of additive manufacturing, especially to produce metal objects, and the awareness of their poor surface quality due to the presence of a significant roughness, have highlighted the need to develop suitable post-processing surface treatments. In this regard, electropolishing techniques are ideal due to their high versatility, even on geometrically complex or small-sized objects, which are difficult to treat with techniques that require physical contact with a tool. On the other hand, the common use of strong and dangerous acid baths does not allow compliance with increasingly stringent sustainability criteria. For this reason, special attention is increasingly directed toward the identification of green electrolytes, based on deep eutectic or acid-free solvents, potentially capable of replacing conventional acid solutions. The choice of new environmentally sustainable and specifically appropriate solvents according to the metal alloys treated could allow a further expansion of the additive processing technologies, and therefore preserve their advantage, extending, among other things, the demand for the related finished products thanks to their superior aesthetic and functional quality.
A Review on Ultrafast Laser Microwelding of Transparent Materials and Transparent Material–Metals
Authors: Xu Jiayi , Jiang Qing , Yang Jin , Cui Jiangmei , Zhao Yixuan , Zheng Min , Oliveira J. P. , Zeng Zhi , Pan Rui , Chen Shujun
Published: May 2023

Transparent hard and brittle (THB) materials have generated significant interest due to their excellent properties, such as wide spectral transmittance, heat resistance, chemical inactivity and high mechanical strength. To further explore the application of THB materials, it is inevitable to be confronted with a range of joining THB materials and THB material–metals. Ultrafast (UF) laser microwelding enables a new means of joining THB materials and THB material–metals, due to a localized energy deposition method, which is dominated by nonlinear absorption. This process can realize high-quality micro-zone direct joining of THB materials or THB material–metals without the assistance of a light-absorbing intermediate layer. In this paper, we review the advances in UF laser microwelding of THB materials and THB material–metals considering the last two decades, from the analysis of the interaction mechanism between UF laser and matter to the key influencing factors and practical applications of this technology. Finally, the existing problems and the future research focus of UF laser microwelding technology of THB materials and THB material–metals are discussed.
The Impact of Metabolic Syndrome Components on Erectile Function in Patients with Type 2 Diabetes
Authors: Katsimardou Alexandra , Patoulias Dimitrios , Zografou Ioanna , Siskos Fotios , Stavropoulos Konstantinos , Imprialos Konstantinos , Tegou Zoi , Boulmpou Aristi , Georgopoulou Vivian , Hatzipapa Nikoleta , Papadopoulos Christodoulos , Doumas Michael
Published: May 2023

Erectile dysfunction is commonly encountered in diabetic patients and in patients with metabolic syndrome; however, only a few studies have assessed patients with metabolic syndrome and type 2 diabetes mellitus (T2DM) regarding their sexual function. The purpose of this study is to examine the effect of metabolic syndrome and its components on the erectile function of T2DM patients. A cross-sectional study including T2DM patients was conducted from November 2018 until November 2020. Participants were evaluated for the presence of metabolic syndrome and their sexual function was assessed using the International Index of Erectile Function (IIEF) questionnaire. A total of 45 consecutive male patients participated in this study. Metabolic syndrome was diagnosed in 84.4% and erectile dysfunction (ED) in 86.7% of them. Metabolic syndrome was not associated with ED or ED severity. Among metabolic syndrome components, only high-density lipoprotein cholesterol (HDL) was associated with ED [x2 (1, n = 45) = 3.894, p = 0.048; OR = 5.5 (95% CI: 0.890–33.99)] and with the IIEF erectile function scores (median 23 vs. 18, U = 75, p = 0.012). Multiple regression analyses showed that HDL was non-significantly associated with the IIEF erectile function scores. In conclusion, among T2DM patients HDL is associated with ED.
Interactive Effect of Dietary Gamma-Aminobutyric Acid (GABA) and Water Temperature on Growth Performance, Blood Plasma Indices, Heat Shock Proteins and GABAergic Gene Expression in Juvenile Olive Flounder Paralichthys olivaceus
Authors: Lee Seunghan , Moniruzzaman Mohammad , Farris Nathaniel , Min Taesun , Bai Sungchul C.
Published: May 2023

Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system of living organisms and has the ability to reduce the magnitude of stress in humans and animals. In this study, we evaluated the supplemental effects of GABA on normal and high water temperature based on growth, blood plasma composition as well as heat shock proteins and GABA-related gene expression in juvenile olive flounder. For this, a 2 × 2 factorial design of experiment was employed to investigate the dietary effects of GABA at 0 mg/kg of diet (GABA0 diet) and 200 mg/kg of diet (GABA200 diet) in water temperatures of 20 ± 1 °C (normal temperature) and 27 ± 1 °C (high temperature) for 28 days. A total of 180 fish with an average initial weight of 40.1 ± 0.4 g (mean ± SD) were distributed into 12 tanks, of which, each tank contained 15 fish based on the 4 dietary treatment groups in triplicate. At the end of the feeding trial, the results demonstrated that both temperature and GABA had significant effects on the growth performance of the fish. However, fish fed the GABA200 diet had a significantly higher final body weight, weight gain and specific growth rate as well as a significantly lower feed conversion ratio than the fish fed the GABA0 diet at the high water temperature. A significant interactive effect of water temperature and GABA was observed on the growth performance of olive flounder based on the two-way analysis of variance. The plasma GABA levels in fish were increased in a dose-dependent manner at normal or high water temperatures, whereas cortisol and glucose levels were decreased in fish fed GABA-supplemented diets under temperature stress. The GABA-related mRNA expression in the brains of the fish such as GABA type A receptor-associated protein (Gabarap), GABA type B receptor 1 (Gabbr1) and glutamate decarboxylase 1 (Gad1) were not significantly affected by GABA-supplemented diets under normal or temperature stressed conditions. On the other hand, the mRNA expression of heat shock proteins (hsp) in the livers of the fish, such as hsp70 and hsp90, were unchanged in fish fed the GABA diets compared to the control diet at the high water temperature. Collectively, the present study showed that dietary supplementation with GABA could enhance growth performance, and improve the feed utilization, plasma biochemical parameters and heat shock proteins and GABA-related gene expression under the stress of high water temperatures in juvenile olive flounder.
Identification of Antimicrobial Metabolites from the Egyptian Soil-Derived Amycolatopsis keratiniphila Revealed by Untargeted Metabolomics and Molecular Docking
Authors: Hamed Ahmed A. , Mohamed Osama G. , Aboutabl Elsayed A. , Fathy Fify I. , Fawzy Ghada A. , El-Shiekh Riham A. , Al-Karmalawy Ahmed A. , Al-Taweel Areej M. , Tripathi Ashootosh , Elsayed Tarek R.
Published: May 2023

Actinomycetes are prolific producers of bioactive secondary metabolites. The prevalence of multidrug-resistant (MDR) pathogens has prompted us to search for potential natural antimicrobial agents. Herein, we report the isolation of rare actinobacteria from Egyptian soil. The strain was identified as Amycolatopsis keratiniphila DPA04 using 16S rRNA gene sequencing. Cultivation profiling, followed by chemical and antimicrobial evaluation of crude extracts, revealed the activity of DPA04 ISP-2 and M1 culture extracts against Gram-positive bacteria. Minimum inhibitory concentrations (MIC) values ranged from 19.5 to 39 µg/mL. Chemical analysis of the crude extracts using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF) led to the identification of 45 metabolites of different chemical classes. In addition, ECO-0501 was identified in the cultures with significant antimicrobial activity. Multidrug resistance in Staphylococcus aureus is reported to be related to the multidrug efflux pump (MATE). ECO-0501 and its related metabolites were subjected to molecular docking studies against the MATE receptor as a proposed mechanism of action. ECO-0501 and its derivatives (AK_1 and N-demethyl ECO-0501) had better binding scores (−12.93, −12.24, and −11.92 kcal/mol) than the co-crystallized 4HY inhibitor (−8.99 kcal/mol) making them promising candidates as MATE inhibitors. Finally, our work established that natural products from this strain could be useful therapeutic tools for controlling infectious diseases.
Donkey Colostrum and Milk: How Dietary Probiotics Can Affect Metabolomic Profile, Alkaline Sphingomyelinase and Alkaline Phosphatase Activity
Authors: Laus Fulvio , Laghi Luca , Bazzano Marilena , Cifone Maria Grazia , Cinque Benedetta , Yang Yaosen , Marchegiani Andrea
Published: May 2023

Positive results on animal health, feed efficiency, and milk’s nutritional content have been obtained after oral administration of probiotics. The aim of the present study was therefore to evaluate the effect of dietary supplementation with high numbers of multispecies probiotic formulations on the milk metabolomic profiles of alkaline sphingomyelinase (alk-SMase) and alkaline phosphatase (ALP) in donkeys. Twenty animals were randomly allocated to receive either a normal diet (group B) or a supplemented diet (group A). Colostrum and milk samples were obtained within 48 h, at 15 days (supplementation start), and at 45 days after parturition. Different metabolomic profiles were observed between colostrum and milk, as were the concentrations of 12 metabolites that changed following 30 days of probiotic supplementation. Alk-SMase activity was found to be higher in donkey colostrum (vs. milk at 15 days); this enzyme, together with ALP, increased in milk after 30 days of probiotic supplementation. The results of the present study provide new insight into the complex changes in donkey colostrum and milk composition in the first 45 days of lactation and how the milk metabolome can be modulated by probiotic supplementation.
Diversities and Shifts of Microbial Communities Associated with Farmed Oysters (Crassostrea gigas) and Their Surrounding Environments in Laoshan Bay Marine Ranching, China
Authors: Fang Guangjie , Yu Haolin , Zhang Yazhou , Liang Jun , Tang Yanli , Liang Zhenlin
Published: May 2023

Pacific oysters (Crassostrea gigas) are widely cultured in Chinese marine ranching with high economic value. However, mass death of farmed oysters has occurred frequently in recent years because of diseases and environmental disturbance (e.g., high temperatures). In order to analyze the potential relationships between microorganisms and the death of farmed oysters, we compared the dynamics of bacterial and protist communities in oysters at different growth phases using high-throughput sequencing. The results showed that the microbial communities in farmed oysters significantly changed and were markedly different from microbes in natural oysters and the surrounding environments. The number of biomarker taxa among farmed oysters and their surrounding environments decreased gradually with the growth of oysters. During the mass death of farmed oysters, the microbial communities’ abundance of ecological function genes changed, and the correlations among microorganisms disappeared. These results enrich our understanding of the dynamics of microbial communities in farmed oysters at different growth phases, illustrating the characteristics of interactions among microorganisms during the mass death of farmed oysters. Our study is beneficial to promote the healthy aquaculture of oysters.
Lignin-Degrading Bacteria in Paper Mill Sludge
Authors: Rodriguez-Yupanqui Magda , De La Cruz-Noriega Magaly , Quiñones Claudio , Otiniano Nélida Milly , Quezada-Alvarez Medardo A. , Rojas-Villacorta Walter , Vergara-Medina Gino A. , León-Vargas Frank R. , Solís-Muñoz Haniel , Rojas-Flores Segundo
Published: May 2023

The effluents generated in the paper industry, such as black liquor, have a high content of lignin and other toxic components; however, they represent a source of lignin-degrading bacteria with biotechnological potential. Therefore, the present study aimed to isolate and identify lignin-degrading bacteria species in paper mill sludge. A primary isolation was carried out from samples of sludge present in environments around a paper company located in the province of Ascope (Peru). Bacteria selection was made by the degradation of Lignin Kraft as the only carbon source in a solid medium. Finally, the laccase activity (Um-L−1) of each selected bacteria was determined by oxidation of 2,2′-azinobis-(3-etilbencenotiazolina-6-sulfonate) (ABTS). Bacterial species with laccase activity were identified by molecular biology techniques. Seven species of bacteria with laccase activity and the ability to degrade lignin were identified. The bacteria Agrobacterium tumefasciens (2), Klebsiella grimontii (1), and Beijeinckia fluminensis (1) were reported for first time. K. grimowntii and B. fluminensis presented the highest laccase activity, with values of 0.319 ± 0.005 UmL−1 and 0.329 ± 0.004 UmL−1, respectively. In conclusion, paper mill sludge may represent a source of lignin-degrading bacteria with laccase activity, and they could have potential biotechnological applications.
Properties of Multidrug-Resistant Mutants Derived from Heterologous Expression Chassis Strain Streptomyces albidoflavus J1074
Authors: Dolya Borys , Hryhorieva Olena , Sorochynska Khrystyna , Lopatniuk Maria , Ostash Iryna , Tseduliak Vasylyna-Marta , Sterndorff Eva Baggesgaard , Jørgensen Tue Sparholt , Gren Tetiana , Dacyuk Yuriy , Weber Tilmann , Luzhetskyy Andriy , Fedorenko Victor , Ostash Bohdan
Published: May 2023

Streptomyces albidoflavus J1074 is a popular platform to discover novel natural products via the expression of heterologous biosynthetic gene clusters (BGCs). There is keen interest in improving the ability of this platform to overexpress BGCs and, consequently, enable the purification of specialized metabolites. Mutations within gene rpoB for the β-subunit of RNA polymerase are known to increase rifampicin resistance and augment the metabolic capabilities of streptomycetes. Yet, the effects of rpoB mutations on J1074 remained unstudied, and we decided to address this issue. A target collection of strains that we studied carried spontaneous rpoB mutations introduced in the background of the other drug resistance mutations. The antibiotic resistance spectra, growth, and specialized metabolism of the resulting mutants were interrogated using a set of microbiological and analytical approaches. We isolated 14 different rpoB mutants showing various degrees of rifampicin resistance; one of them (S433W) was isolated for the first time in actinomycetes. The rpoB mutations had a major effect on antibiotic production by J1074, as evident from bioassays and LC-MS data. Our data support the idea that rpoB mutations are useful tools to enhance the ability of J1074 to produce specialized metabolites.
Occurrence of Leishmaniasis in Iberian Wolves in Northwestern Spain
Authors: Merino Goyenechea Javier , Castilla Gómez de Agüero Verónica , Palacios Alberti Jesús , Balaña Fouce Rafael , Martínez Valladares María
Published: May 2023

Canine leishmaniasis is an important vector-borne protozoan disease in dogs that is responsible for serious deterioration in their health. In the Iberian Peninsula, as in most countries surrounding the Mediterranean Sea, canine leishmaniasis is caused by Leishmania infantum (zymodeme MON-1), a digenetic trypanosomatid that harbors in the parasitophorous vacuoles of host macrophages, causing severe lesions that can lead to death if the animals do not receive adequate treatment. Canine leishmaniasis is highly prevalent in Spain, especially in the Mediterranean coastal regions (Levante, Andalusia and the Balearic Islands), where the population of domestic dogs is very high. However, the presence of this disease has been spreading to other rural and sparsely populated latitudes, and cases of leishmaniasis have been reported for years in wildlife in northwestern Spain. This work describes for the first time the presence of wolves that tested positive for leishmaniasis in the vicinity of the Sierra de la Culebra (Zamora province, northwestern Spain), a protected sanctuary of this canid species, using PCR amplification of L. infantum DNA from different non-invasive samples such as buccal mucosa and those from both ears and hair. In addition to live animals (21), samples from carcasses of mainly roadkill animals (18) were also included and analyzed using the same technique, obtaining a positivity rate of 18 of the 39 wolves sampled (46.1%) regardless of their origin.
Microbiome of Ceca from Broiler Chicken Vaccinated or Not against Coccidiosis and Fed Berry Pomaces
Authors: Yang Chongwu , Das Quail , Rehman Muhammad A. , Yin Xianhua , Shay Julie , Gauthier Martin , Lau Calvin Ho-Fung , Ross Kelly , Diarra Moussa S.
Published: May 2023

American cranberry (Vaccinium macrocarpon) and lowbush/wild blueberry (V. angustifolium) pomace are polyphenol-rich products having potentially beneficial effects in broiler chickens. This study investigated the cecal microbiome of broiler-vaccinated or non-vaccinated birds against coccidiosis. Birds in each of the two groups (vaccinated or non-vaccinated) were fed a basal non-supplemented diet (NC), a basal diet supplemented with bacitracin (BAC), American cranberry (CP), and lowbush blueberry (BP) pomace alone or in combination (CP + BP). At 21 days of age, cecal DNA samples were extracted and analyzed using both whole-metagenome shotgun sequencing and targeted-resistome sequencing approaches. Ceca from vaccinated birds showed a lower abundance of Lactobacillus and a higher abundance of Escherichia coli than non-vaccinated birds (p < 0.05). The highest and lowest abundance of L. crispatus and E. coli, respectively, were observed in birds fed CP, BP, and CP + BP compared to those from NC or BAC treatments (p < 0.05). Coccidiosis vaccination affected the abundance of virulence genes (VGs) related to adherence, flagella, iron utilization, and secretion system. Toxin-related genes were observed in vaccinated birds (p < 0.05) in general, with less prevalence in birds fed CP, BP, and CP + BP than NC and BAC (p < 0.05). More than 75 antimicrobial resistance genes (ARGs) detected by the shotgun metagenomics sequencing were impacted by vaccination. Ceca from birds fed CP, BP, and CP + BP showed the lowest (p < 0.05) abundances of ARGs related to multi-drug efflux pumps, modifying/hydrolyzing enzyme and target-mediated mutation, when compared to ceca from birds fed BAC. Targeted metagenomics showed that resistome from BP treatment was distant to other groups for antimicrobials, such as aminoglycosides (p < 0.05). Significant differences in the richness were observed between the vaccinated and non-vaccinated groups for aminoglycosides, β-lactams, lincosamides, and trimethoprim resistance genes (p < 0.05). Overall, this study demonstrated that dietary berry pomaces and coccidiosis vaccination significantly impacted cecal microbiota, virulome, resistome, and metabolic pathways in broiler chickens.
Effect of Anthropogenic Disturbances on the Microbial Relationship during Bioremediation of Heavy Metal-Contaminated Sediment
Authors: Yang Quanliu , Jie Shiqi , Lei Pan , Gan Min , He Peng , Zhu Jianyu , Zhou Qingming
Published: May 2023

Soil, sediment, and waters contaminated with heavy metals pose a serious threat to ecosystem function and human health, and microorganisms are an effective way to address this problem. In this work, sediments containing heavy metals (Cu, Pb, Zn, Mn, Cd, As) were treated differently (sterilized and unsterilized) and bio-enhanced leaching experiments were carried out with the addition of exogenous iron-oxidizing bacteria A. ferrooxidans and sulfur-oxidizing bacteria A. thiooxidans. The leaching of As, Cd, Cu, and Zn was higher in the unsterilized sediment at the beginning 10 days, while heavy metals leached more optimally in the later sterilized sediment. The leaching of Cd from sterilized sediments was favored by A. ferrooxidans compared to A. thiooxidans. Meanwhile, the microbial community structure was analyzed using 16S rRNA gene sequencing, which revealed that 53.4% of the bacteria were Proteobacteria, 26.22% were Bacteroidetes, 5.04% were Firmicutes, 4.67% were Chlamydomonas, and 4.08% were Acidobacteria. DCA analysis indicated that microorganisms abundance (diversity and Chao values) increased with time. Furthermore, network analysis showed that complex networks of interactions existed in the sediments. After adapting to the acidic environmental conditions, the growth of some locally dominant bacteria increased the microbial interactions, allowing more bacteria to participate in the network, making their connections stronger. This evidence points to a disruption in the microbial community structure and its diversity following artificial disturbance, which then develops again over time. These results could contribute to the understanding of the evolution of microbial communities in the ecosystem during the remediation of anthropogenically disturbed heavy metals.
Phytoplankton–Bacteria Interactions 1.0
Authors: Petrou Katherina
Published: May 2023

Phytoplankton and bacteria regulate many essential functions in aquatic ecosystems [...]
Special Issue “Functional Characterization of Lactic Acid Bacteria”: Editorial
Authors: Rossi Franca
Published: May 2023

Lactic acid bacteria (LAB) are a diverse group of microorganisms of the order Lactobacillales in the Bacillota phylum, subdivision Bacilli, comprising, at this stage of taxonomic descriptions six families (Aerococcaceae, Carnobacteriaceae, Enterococcaceae, Lactobacillaceae, Leuconostocaceae and Streptococcaceae) [...]
Influence of Sodium Carbonate on the Flotation Separation of Smithsonite and Calcite by Sulfuration-Amine Method
Authors: Piao Yongchao , He Fayu , Pang Zengrui , Liu Taishun , Shang Yanbo , Luo Kehua , Zhu Yangge
Published: May 2023

Sulfuration-amine flotation is the most commonly used method to separate zinc oxide ore, but its shortcomings such as unstable separation index and poor applicability to high mud-content raw ore limit its application in industry. In this study, the influence mechanism of sodium carbonate on the flotation separation of smithsonite and calcite by the sulfuration-amine method was investigated by chemical analysis of the flotation solution, contact angle measurement, Zeta potential test, and XPS analysis. The results showed that sodium carbonate significantly improved the flotation separation performance of smithsonite and calcite. The chemical analysis of the flotation solution showed that sodium carbonate hindered the dissolution of smithsonite, reducing its negative impact on the flotation of smithsonite. The results of the zeta potential and XPS tests showed that sodium carbonate enhanced the electronegativity of the smithsonite surface, and in an alkaline environment, sodium carbonate was favorable for the adsorption of dodecylamine on the surface of smithsonite, while sodium carbonate and high alkalinity enhanced the inhibitory effect of sodium hexametaphosphate on calcite. The study proved that sodium carbonate could be an effective modifier to promote the flotation separation of smithsonite and calcite using the aulfuration-amine method.
Development of Mid-Infrared Absorption Spectroscopy for Gemstone Analysis
Authors: Wang Zhen , Takahashi Hiroshi
Published: May 2023

Absorption spectroscopy has been widely used in gemstone societies as it enables the identification of the origin and post-treatment of gemstones in a non-destructive way. The infrared (IR) range is critical for studies of gemstones such as corundum, emerald, and diamond. Regarding the corundum sample, absorption peaks related to the -OH bond could be used as an index for heat-treatment detection, and different types of inclusions could be identified by analyzing the IR absorption spectrum. The most widely used method for measuring IR absorptions is Fourier-transform infrared spectroscopy (FTIR), which was designed based on the working of the Michelson interferometer. However, FTIR has a few limitations, such as a long measurement time and difficulty in sample placement, which limits its full automation capability. In this study, a mid-infrared (MIR) spectrometer relying on the upconversion phenomenon of non-linear crystals was used to measure the absorption spectra. Corundum with heat treatment features and/or with different common types of inclusions were measured by both transmission and reflection modes. After comparison with the FTIR spectra captured on the same sample set, the same target peaks could be captured with a shorter measurement time and easier operation. The developed MIR spectrometer could directly measure the absorption spectrum in the 2–4.5 µm (2200–5000 cm−1) range within a few seconds. Meanwhile, as both transmission and reflection modes were available, both loose and mounted gemstones could be measured, supporting the widespread use of this device in large-scale production and its ability to achieve full automation.
Rapid Estimation of Sulfur Content in High-Ash Indian Coal Using Mid-Infrared FTIR Data
Authors: Shukla Anubhav , Prasad Anup K. , Mishra Sameeksha , Vinod Arya , Varma Atul K.
Published: May 2023

High-ash Indian coals are primarily used as thermal coal in power plants and industries. Due to the presence of sulfur in thermal coal, flue gas is a major environmental concern. Conventional methods (Ultimate Analysis of Coal) for sulfur content estimation are time-consuming, relatively costly, and destructive. In this study, Fourier-transform infrared (FTIR) spectroscopy has emerged as a promising alternative method for the rapid and nondestructive analysis of the sulfur content in coal. In the present study, the actual sulfur content in the coal samples was determined using Ultimate Analysis (CHNS analyzer). In contrast, mid-infrared FTIR spectroscopic data (4000–400 cm−1) were used to analyze the functional groups related to sulfur or its compounds in the coal samples to predict the sulfur content. A comparison of sulfur estimated using a CHNS analyzer and predicted using mid-infrared spectroscopy (FTIR) data shows that it can accurately predict sulfur content in high-ash Indian coals using the piecewise linear regression method (Quasi-Newton, QN). The proposed FTIR-based sulfur prediction model showed a coefficient of determination (R2) of up to 0.93, where the total no. of samples (Coal + KBr pellets, n) was 126 (using 17:1 split, K-fold cross validation). The root-mean-square error (RMSE, wt.%) is 0.0035, mean bias error (MBE, wt.%) is −0.0003, MBE (%) is 3.31% and mean absolute error (MAE, wt.%) is 0.0020. The two-tailed t-test and F-test for mean and variance indicated no significant difference between the pair of values of observed sulfur (SCHNS, wt.%) using CHNS data and the model predicted sulfur (SFTIR, wt.%) using FTIR data. The prediction model using mid-infrared FTIR spectroscopy data and the Quasi-Newton method with a breakpoint and loss function performs well for coal samples from the Johilla Coalfield, Umaria. Thus, it can be a valuable tool for analyzing sulfur in other ash-rich coals from various basins worldwide.