bluuebunny/crossref_metadata_2025 huggingface dataset:
Straw Return and Nitrogen Fertilization to Maize Regulate Soil Properties, Microbial Community, and Enzyme Activities Under a Dual Cropping System
Authors: Yang Li , Muhammad Ihsan , Chi Yu Xin , Wang Dan , Zhou Xun Bo
Published: March 2022

Soil sustainability is based on soil microbial communities’ abundance and composition. Straw returning (SR) and nitrogen (N) fertilization influence soil fertility, enzyme activities, and the soil microbial community and structure. However, it remains unclear due to heterogeneous composition and varying decomposition rates of added straw. Therefore, the current study aimed to determine the effect of SR and N fertilizer application on soil organic carbon (SOC), total nitrogen (TN), urease (S-UE) activity, sucrase (S-SC) activity, cellulose (S-CL) activity, and bacterial, fungal, and nematode community composition from March to December 2020 at Guangxi University, China. Treatments included two planting patterns, that is, SR and traditional planting (TP) and six N fertilizer with 0, 100, 150, 200, 250, and 300 kg N ha–1. Straw returning significantly increased soil fertility, enzymatic activities, community diversity, and composition of bacterial and fungal communities compared to TP. Nitrogen fertilizer application increased soil fertility and enzymes and decreased the richness of bacterial and fungal communities. In SR added plots, the dominated bacterial phyla were Proteobacteria, Acidobacterioia, Nitrospirae, Chloroflexi, and Actinobacteriota; whereas fungal phyla were Ascomycota and Mortierellomycota and nematode genera were Pratylenchus and Acrobeloides. Co-occurrence network and redundancy analysis (RDA) showed that TN, SOC, and S-SC were closely correlated with bacterial community composition. It was concluded that the continuous SR and N fertilizer improved soil fertility and improved soil bacterial, fungal, and nematode community composition.
A Novel NADP-Dependent Formate Dehydrogenase From the Hyperthermophilic Archaeon Thermococcus onnurineus NA1
Authors: Yang Ji-in , Lee Seong Hyuk , Ryu Ji-Young , Lee Hyun Sook , Kang Sung Gyun
Published: March 2022

The genome of the hyperthermophilic archaeon Thermococcus onnurineus NA1 contains three copies of the formate dehydrogenase (FDH) gene, fdh1, fdh2, and fdh3. Previously, we reported that fdh2, clustered with genes encoding the multimeric membrane-bound hydrogenase and cation/proton antiporter, was essential for formate-dependent growth with H2 production. However, the functionality of the other two FDH-coding genes has not yet been elucidated. Herein, we purified and characterized cytoplasmic Fdh3 to understand its functionality. The purified Fdh3 was identified to be composed of a tungsten-containing catalytic subunit (Fdh3A), an NAD(P)-binding protein (Fdh3B), and two Fe-S proteins (Fdh3G1 and Fdh3G2). Fdh3 oxidized formate with specific activities of 241.7 U/mg and 77.4 U/mg using methyl viologen and NADP+ as electron acceptors, respectively. While most FDHs exhibited NAD+-dependent formate oxidation activity, the Fdh3 of T. onnurineus NA1 showed a strong preference for NADP+ over NAD+ as a cofactor. The catalytic efficiency (kcat/Km) of Fdh3 for NADP+ was measured to be 5,281 mM−1 s−1, which is the highest among NADP-dependent FDHs known to date. Structural modeling suggested that Arg204 and Arg205 of Fdh3B may contribute to the stabilization of the 2′-phosphate of NADP(H). Fdh3 could also use ferredoxin as an electron acceptor to oxidize formate with a specific activity of 0.83 U/mg. Furthermore, Fdh3 showed CO2 reduction activity using reduced ferredoxin or NADPH as an electron donor with a specific activity of 0.73 U/mg and 1.0 U/mg, respectively. These results suggest a functional role of Fdh3 in disposing of reducing equivalents by mediating electron transfer between formate and NAD(P)H or ferredoxin.
Development and Validation of a 7-Gene Inflammatory Signature Forecasts Prognosis and Diverse Immune Landscape in Lung Adenocarcinoma
Authors: Nai Aitao , Ma Feng , He Zirui , Zeng Shuwen , Bashir Shoaib , Song Jian , Xu Meng
Published: March 2022

Background: Inflammatory responses are strongly linked with tumorigenesis and cancer development. This research aimed to construct and validate a novel inflammation response–related risk predictive signature for forecasting the prognosis of patients with LUAD.

Methods: Differential expression analysis, univariate Cox, LASSO, and multivariate Cox regression analyses of 200 inflammatory response–related genes (IRRG) were performed to establish a risk predictive model in the TCGA training cohort. The performance of the IRRG model was verified in eight GEO datasets. GSEA analysis, ESTIMATE algorithms, and ssGSEA analysis were applied to elucidate the possible mechanisms. Furthermore, the relationship analysis between risk score, model genes, and chemosensitivity was performed. Last, we verified the protein expression of seven model genes by immunohistochemical staining or Western blotting.

Results: We constructed a novel inflammatory response–related 7-gene signature (MMP14, BTG2, LAMP3, CCL20, TLR2, IL7R, and PCDH7). Patients in the high-risk group presented markedly decreased survival time in the TCGA cohort and eight GEO cohorts than the low-risk group. Interestingly, multiple pathways related to immune response were suppressed in high-risk groups. The low infiltration levels of B cell, dendritic cell, natural killer cell, and eosinophil can significantly affect the unsatisfactory prognosis of the high-risk group in LUAD. Moreover, the tumor cells’ sensitivity to anticancer drugs was markedly related to risk scores and model genes. The protein expression of seven model genes was consistent with the mRNA expression.

Conclusion: Our IRRG prognostic model can effectively forecast LUAD prognosis and is tightly related to immune infiltration.
Structural Dissection of the First Events Following Membrane Binding of the Islet Amyloid Polypeptide
Authors: Khemtemourian Lucie , Fatafta Hebah , Davion Benoit , Lecomte Sophie , Castano Sabine , Strodel Birgit
Published: March 2022

The islet amyloid polypeptide (IAPP) is the main constituent of the amyloid fibrils found in the pancreas of type 2 diabetes patients. The aggregation of IAPP is known to cause cell death, where the cell membrane plays a dual role: being a catalyst of IAPP aggregation and being the target of IAPP toxicity. Using ATR-FTIR spectroscopy, transmission electron microscopy, and molecular dynamics simulations we investigate the very first molecular steps following IAPP binding to a lipid membrane. In particular, we assess the combined effects of the charge state of amino-acid residue 18 and the IAPP-membrane interactions on the structures of monomeric and aggregated IAPP. Distinct IAPP-membrane interaction modes for the various IAPP variants are revealed. Membrane binding causes IAPP to fold into an amphipathic α-helix, which in the case of H18K-, and H18R-IAPP readily moves beyond the headgroup region. For all IAPP variants but H18E-IAPP, the membrane-bound helix is an intermediate on the way to amyloid aggregation, while H18E-IAPP remains in a stable helical conformation. The fibrillar aggregates of wild-type IAPP and H18K-IAPP are dominated by an antiparallel β-sheet conformation, while H18R- and H18A-IAPP exhibit both antiparallel and parallel β-sheets as well as amorphous aggregates. Our results emphasize the decisive role of residue 18 for the structure and membrane interaction of IAPP. This residue is thus a good therapeutic target for destabilizing membrane-bound IAPP fibrils to inhibit their toxic actions.
Development and Validation of a Multimorbidity Index Predicting Mortality Among Older Chinese Adults
Authors: Luo Yan , Huang Ziting , Liu Hui , Xu Huiwen , Su Hexuan , Chen Yuming , Hu Yonghua , Xu Beibei
Published: March 2022

Objective

This study aimed to develop and validate a multimorbidity index using self-reported chronic conditions for predicting 5-year mortality risk.

Methods

We analyzed data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) and included 11,853 community-dwelling older adults aged 65–84 years. Restrictive association rule mining (ARM) was used to identify disease combinations associated with mortality based on 13 chronic conditions. Data were randomly split into the training (N = 8,298) and validation (N = 3,555) sets. Two multimorbidity indices with individual diseases only (MI) and disease combinations (MIDC) were developed using hazard ratios (HRs) for 5-year morality in the training set. We compared the predictive performance in the validation set between the models using condition count, MI, and MIDC by the concordance (C) statistic, the Integrated Discrimination Improvement (IDI), and the Net Reclassification Index (NRI).

Results

A total of 13 disease combinations were identified. Compared with condition count (C-statistic: 0.710), MIDC (C-statistic: 0.713) showed significantly better discriminative ability (C-statistic: p = 0.016; IDI: 0.005, p < 0.001; NRI: 0.038, p = 0.478). Compared with MI (C-statistic: 0.711), the C-statistic of the model using MIDC was significantly higher (p = 0.031), while the IDI was more than 0 but not statistically significant (IDI: 0.003, p = 0.090).

Conclusion

Although current multimorbidity status is commonly defined by individual chronic conditions, this study found that the multimorbidity index incorporating disease combinations showed supreme performance in predicting mortality among community-dwelling older adults. These findings suggest a need to consider significant disease combinations when measuring multimorbidity in medical research and clinical practice.
Anti-thyroglobulin Antibodies as a Possible Risk Factor for Graves' Disease After Radioiodine Treatment for Toxic Nodular Goiter: Case Report
Authors: Rouiller Nathalie , Nicod Lalonde Marie , Sykiotis Gerasimos P.
Published: March 2022

Background

The manifestation of Graves' disease (GD) in patients treated with radioactive iodine (RAI) for hyperfunctioning thyroid nodules (RAI-induced GD or post-RAI GD) remains a long-standing challenge in radionuclide therapy. Known risk factors for post-RAI GD include preexisting subclinical hyperthyroidism, positive thyroid peroxidase autoantibodies (TPOAb), positive TSH receptor autoantibodies (TRAb) or otherwise undiagnosed GD. However, these risk factors are not present in all patients with post-RAI GD, and therefore it cannot always be predicted in a reliable manner if a given patient has a high risk for RAI-induced GD or not.

Case Presentation

We describe the case of a 64 year-old woman known for hyperthyroidism due to toxic nodular goiter; she was treated initially with carbimazole, and then, due to recurrence, underwent RAI treatment. Three months later, symptomatic hyperthyroidism persisted. Diagnosis of new-onset GD was made based on typical ultrasound findings and newly-positive TRAb. Our patient had only positive thyroglobulin antibodies (TgAb) before RAI treatment, whereas TPOAb were negative.

Conclusions

In the literature, TgAb have never been reported as a possible risk factor for RAI-induced GD. The present case suggests that the assessment for pre-existing autoimmunity in patients considering RAI for hyperfunctioning thyroid nodules should probably also include TgAb.
Purification and Characterization of Novel Antioxidative Peptides From Duck Liver Protein Hydrolysate as Well as Their Cytoprotection Against Oxidative Stress in HepG2 Cells
Authors: Sun Jin , Zhou Changyu , Cao Jinxuan , He Jun , Sun Yangying , Dang Yali , Pan Daodong , Xia Qiang
Published: March 2022

This study aimed at mining antioxidant peptides derived from duck liver as a strategy for valorizing poultry byproducts utilization via the isolation and characterization of peptide molecules with great antioxidant potential and cytoprotective effects against hydrogen peroxide-induced oxidative stress. Six novel peptides, including GEHGDSSVPVWSGVN, HLDYYLGK, HLTPWIGK, DTYIRQPW, WDDMEKIWHH, and MYPGIAD were isolated and purified by Sephadex G-15 and reverse-phase high-performance liquid chromatography, followed by the identification with liquid chromatography-tandem mass spectrometry. Among the hydrolysates from different enzymes, the alcalase-originated peptides presented the strongest antioxidant capacity as revealed by DPPH and ABTS assays. The synthesized peptides were used to validate the antioxidant activities, identifying that DTYIRQPW and WDDMEKIWHH were the major antioxidative peptides capable of protecting HepG2 cells from H2O2-induced oxidative damage via stimulating antioxidant enzymes such as superoxide dismutase and catalase to eliminate free radicals and to decrease lipid peroxidation products. Molecular docking suggested that the antioxidative properties of the isolated peptides were related to the site and number of hydrogen bonds. This investigation indicated the great potential of duck liver protein hydrolysates as a base material for producing and developing dietary bioactive peptides.
Clinical and Laboratory Features of PCR-Confirmed and Clinically Suspected COVID-19 Pediatric Patients: A Single Hospital-Based Experience During the First COVID-19 Wave in the United Arab Emirates
Authors: Eldin Nashwa M. B. , Saleh Maysa , Labib Bahaaeldin , Othman Marwa , Chacko Lalu , Mae Daphne , Elnour Lamiaa , Al-Rifai Rami H.
Published: March 2022

Objective

This study investigated clinical and laboratory differences between confirmed (RT-PCR-positive) and clinically suspected (RT-PCR-negative) COVID-19 pediatric patients, and explored factors associated with disease severity at presentation and duration of hospitalization.

Methods

Medical charts of COVID-19-confirmed and clinically suspected pediatric patients admitted to a tertiary hospital in Abu Dhabi were reviewed. Sociodemographic information and clinical and laboratory outcomes were retrieved and analyzed.

Results

Between 1 April to 30 June, 2020, 173 patients (mean age: 3.6 ± SD 3.2 years) presented with respiratory symptoms. Of them, 18.0% had confirmed contact with COVID-19 cases, 66.5% had symptoms for ≤3 days, and 86.7% were with moderate to severe disease. Twenty-eight (16.1%) patients tested positive while the rest (83.8%) tested negative in RT-PCR. COVID-19-confirmed and clinically suspected patients were statistically similar (p > 0.05) in all sociodemographic data, disease severity, and vital signs except residence status (89.3% vs. 58.6% were residents, respectively, p = 0.002) and contact with confirmed COVID-19 cases (82.1% vs. 5.5%, respectively, p < 0.001). Fever (100 and 91.0%) and cough (100 and 95.9%) were the most common symptoms in both confirmed and clinically suspected COVID-19 patients. All patients were statistically comparable in mean white blood cell and platelet counts and hemoglobin concentration, except in mean concentration of neutrophils (higher in clinically suspected, p = 0.019). C-reactive protein was two times higher in clinically suspected compared to confirmed patients (p = 0.043). Lymphocyte (OR: 1.31, p < 0.001), LDH (OR: 1.01, p = 0.001), D-dimer (OR: 1.92, p < 0.001), and ferritin levels after 24–36 h (OR: 9.25, p < 0.05), and SGPT (OR: 1.04, p < 0.05) were all associated with disease severity. Elevated ferritin (>300 μg/L) after 24–36 h was the only correlated factor with disease severity (aOR: 17.38, p < 0.05). Confirmed compared with clinically suspected patients (aOR: 4.00, 95% CI: 2.92–5.10) and children with moderate compared with mild disease (aOR: 5.87, 95% CI: 1.08–32.06) had longer hospitalization.

Conclusion

In pediatric patients with negative RT-PCR, COVID-19 is still suspected based on clinical symptoms and epidemiological data. A tentative diagnosis can be made based on a thorough examination, and proper medical management can be initiated promptly.
Da-Chai-Hu-Tang Protects From Acute Intrahepatic Cholestasis by Inhibiting Hepatic Inflammation and Bile Accumulation via Activation of PPARα
Authors: Xu Shihao , Qiao Xi , Peng Peike , Zhu Ziyi , Li Yaoting , Yu Mengyuan , Chen Long , Cai Yin , Xu Jin , Shi Xinwei , Proud Christopher G. , Xie Jianling , Shen Kaikai
Published: March 2022

Cholestasis is caused by intrahepatic retention of excessive toxic bile acids and ultimately results in hepatic failure. Da-Chai-Hu-Tang (DCHT) has been used in China to treat liver and gallbladder diseases for over 1800 years. Here, we demonstrated that DCHT treatment prevented acute intrahepatic cholestasis with liver injury in response to α-naphthylisothiocyanate (ANIT) not to bile duct ligation (BDL) induced-extrahepatic cholestasis. ANIT (80 mg/kg) increased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), direct bilirubin (DBiL), total bilirubin (TBiL), and total bile acids (TBA) which was attenuated by DCHT treatment in a dose-dependent manner. DCHT treatment at high dose of 1.875 g/kg restored bile acid homeostasis, as evidenced by the recovery of the transcription of genes implicated in bile acid biosynthesis, uptake and efflux. DCHT treatment (1.875 g/kg) reversed ANIT-evoked disordered glutathione homeostasis (as determined by GSH/GSSG ratio) and increased in the mRNA levels for Il6, Il1b and Tnfa associated with liver inflammation. Using network pharmacology-based approaches, we identified 22 putative targets involved in DCHT treatment for intrahepatic cholestasis not extrahepatic cholestasis. In addition, as evidenced by dual-luciferase reporter assays, compounds from DCHT with high affinity of PPARα increased luciferase levels from a PPARα-driven reporter. PPARα agonist fenofibrate was able to mimic the cytoprotective effect of DCHT on intrahepatic cholestasis, which was abolished by the PPARα antagonist GW6471. KEGG enrichment and western blot analyses showed that signaling axes of JNK/IL-6/NF-κB/STAT3 related to PPARα might be the principal pathway DCHT affects intrahepatic cholestasis. Taken together, the present study provides compelling evidence that DCHT is a promising formula against acute intrahepatic cholestasis with hepatotoxicity which works via PPARα activation.
Combined Metabolome and Transcriptome Analyses Reveal the Flavonoids Changes and Biosynthesis Mechanisms in Different Organs of Hibiseu manihot L.
Authors: Zhou Yuhan , Xu Xiaodi , Chen Yanzhu , Gao Jun , Shi Qiyu , Tian Liang , Cao Li
Published: March 2022

Hibiseu manihot L. (Jinhuakui, JHK), also known as a garden landscape plant, is widely cultivated as a landscape plant having pharmacological effects due to its high flavonoids content. Although flavonoids were the main active pharmaceutical ingredients in JHK, little information was obtained about the content, composition, and accumulation pattern of flavonoids in different tissues. Most studies only identified a few kinds of flavonoids in JHK limited by separation and identification problems. Therefore, combined metabolome and transcriptome analysis was performed to explore the accumulation patterns and biosynthesis mechanisms of flavonoids in JHK. In this study, we identified 160 flavonoids in 15 samples of JHK (flower, leaf, root, stem, and seeds) by using LC-MS/MS. Consistent with the total flavonoid content determination, these flavonoids were significantly accumulated in flowers, followed by leaves, stems, roots, and seeds. Among them, certain flavonoids, with high content, were also identified for the first time in JHK, such as tricetin, catechin, hesperidin, ncyanidin-3-O-sambubioside, astragalin, procyanidin B2/B3/C1, apigenin-5-O-glucoside, etc. Different tissues underwent significantly reprogramming of their transcriptomes and metabolites changes in JHK, particularly in the flavonoid, flavone, and flavonol biosynthesis pathways. We conducted a correlation analysis between RNA-seq and LC-MS/MS to identify the key genes and related flavonoids compounds, rebuild the gene-metabolites regulatory subnetworks, and then identified 15 key genes highly related to flavonoids accumulation in JHK. These key genes might play a fine regulatory role in flavonoids biosynthesis by affecting the gene expression level in different organs of JHK. Our results could be helpful for the improvement of the market/industrial utilization value of different parts of JHK, to pave the way for the regulatory mechanism research of flavonoids biosynthesis, and provide insight for studying the production quality improvement of JHK.
Applying Social Cognitive Theory in Predicting Physical Activity Among Chinese Adolescents: A Cross-Sectional Study With Multigroup Structural Equation Model
Authors: Liu Jianxiu , Zeng Muchuan , Wang Dizhi , Zhang Yao , Shang Borui , Ma Xindong
Published: March 2022

This cross-sectional study aimed to assess the applicability of social cognitive determinants among the Chinese adolescents and examine whether the predictability of the social cognitive theory (SCT) model on physical activity (PA) differs across gender (boys and girls) and urbanization (urban and suburban). A total of 3,000 Chinese adolescents ranging between the ages of 12–15 years were randomly selected to complete a set of questionnaires. Structural equation modeling (SEM) was applied to investigate the relationships between social cognitive variables and PA in the urbanization and gender subgroups. The overall model explained 38.9% of the variance in PA. Fit indices indicated that the structural model of SCT was good: root mean square error of approximation (RMSEA) = 0.047, (root mean square residual) RMR = 0.028, goodness of fit index (GFI) = 0.974, adjusted goodness of fit index (AGFI) = 0.960, Tucker–Lewis coefficient (TLI) = 0.971, and comparative fit index (CFI) = 0.978. Regarding the subgroup analysis, social support (critical ratios [CRs] = 2.118; p < 0.001) had a more substantial impact on the PA of adolescents in suburban areas than that in urban areas, whereas self-regulation (CRs = −2.896, p < 0.001) had a more substantial impact on the PA of adolescents in urban areas than in suburban areas. The results indicate that the SCT model predicts the PA of Chinese adolescents substantially. An SCT model could apply over a range of subgroups to predict the PA behavior and should be considered comprehensively when designing interventions. These findings would benefit PA among the Chinese adolescents, especially across genders and urbanization.
Self-Perception or Objective State: A Further Study of the Effects of Retirement on Health
Authors: Tang Yuanmao , Liu Danping , Mou Shaobo , Isa Salmi Mohd , Gui Siyuan , Wan Qin
Published: March 2022

Against the backdrop of an aging global population and the increasing pressure of medical care expenditures for seniors, this paper used a fuzzy regression discontinuity (FRD) model to explore the effects of retirement on the self-assessed health and objective physical and mental health of older people. Using survey data from the China Health and Retirement Longitudinal Study (CHARLS), our model addresses some relevant academic controversies. Our sample was comprised of male respondents from government agencies, enterprises, and public institutions. The research explored the impact of retirement on lifestyle habits and included an in-depth analysis of the mechanism through which retirement influences different aspects of health. The results show that: (1) Retirement does not have any significant impact on objective health, including depression and self-care ability, but it does cause a notable decline in subjective health assessment. (2) Retirement shortened the sleep time of respondents, which may account for lower scores on subjective health self-evaluations, but it did not lead to any noticeable improvement in habits which are harmful to health, such as smoking and drinking. (3) Marriage can help alleviate the problems of depression and smoking among older people, and education has a somewhat broader positive effect on their health and lifestyles; however, neither factor helps to improve the sleep problems of older people. Therefore, this paper recommends that efforts should be made to both optimize retirement policies and seek further ways to improve the health of the retired population.
Effects of Naturalistic Psychedelic Use on Depression, Anxiety, and Well-Being: Associations With Patterns of Use, Reported Harms, and Transformative Mental States
Authors: Raison Charles L. , Jain Rakesh , Penn Andrew D. , Cole Steven P. , Jain Saundra
Published: March 2022

Survey-based studies suggest naturalistic psychedelic use provides mental health benefits similar to those observed in clinical trials. The current study sought to confirm these findings in a large group of psychedelic users and to conduct a novel examination of associations between amount of psychedelic use and behavioral outcomes, as well as frequency of harms ascribed to psychedelic use. A cross-sectional, online survey was completed by 2,510 adults reporting at least one lifetime psychedelic experience. Participants retrospectively completed a battery of instruments assessing depression, anxiety, and emotional well-being prior to and following psychedelic exposure. Participants also reported preferred psychedelic agent, number of uses, and harms attributed to psychedelic use. Psychedelic use was associated with significant improvements in depressive and anxious symptoms and with increased emotional well-being. These improvements increased in magnitude with increasing psychedelic exposure, with a ceiling effect. However, improvements were noted following a single lifetime use. Strong evidence for benefit of one preferred psychedelic agent over another was not observed, but enduring increases in factors related to mystical-experience and prosocial perspective taking associated with enhanced mental health. Thirteen percent of the survey sample (n = 330) endorsed at least one harm from psychedelic use, and these participants reported less mental health benefit. Results from the current study add to a growing database indicating that psychedelic use—even outside the context of clinical trials—may provide a wide range of mental health benefits, while also posing some risk for harm in a minority of individuals.
BDNF and Cortisol in the Diagnosis of Cocaine-Induced Depression
Authors: Fonseca Francina , Mestre-Pinto Joan Ignasi , Rodríguez-Minguela Rocío , Papaseit Esther , Pérez-Mañá Clara , Langohr Klaus , Barbuti Margherita , Farré Magí , Torrens Marta ,   NEURODEP GROUP
Published: March 2022

Background

Major depressive disorder (MDD) and cocaine use disorder (CUD) are related with disability and high mortality rates. The assessment and treatment of psychiatric comorbidity is challenging due to its high prevalence and its clinical severity, mostly due to suicide rates and the presence of medical comorbidities. The aim of this study is to investigate differences in brain derived neurotrophic factor (BDNF) and cortisol plasmatic levels in patients diagnosed with CUD-primary-MDD and CUD-induced-MDD and also to compare them to a sample of MDD patients (without cocaine use), a sample of CUD (without MDD), and a group of healthy controls (HC) after a stress challenge.

Methods

A total of 46 subjects were included: MDD (n = 6), CUD (n = 15), CUD-primary-MDD (n = 16), CUD-induced-MDD (n = 9), and 21 HC. Psychiatric comorbidity was assessed with the Spanish version of the Psychiatric Research Interview for Substance and Mental Disorders IV (PRISM-IV), and depression severity was measured with the Hamilton Depression Rating Scale (HDRS). Patients were administered the Trier Social Stress Test (TSST) before and after the biological measures, including BDNF, and cortisol levels were obtained.

Results

After the TSST, Cohen's d values between CUD-primary-MDD and CUD-induced-MDD increased in each assessment from 0.19 post-TSST to 2.04 post-90-TSST. Pairwise differences among CUD-induced-MDD and both MDD and HC groups had also a large effect size value in post-30-TSST and post-90-TSST. In the case of the BDNF concentrations, CUD-primary-MDD and CUD-induced-MDD in post-90-TSST (12,627.27 ± 5488.09 vs.17,144.84 ± 6581.06, respectively) had a large effect size (0.77).

Conclusion

Results suggest a different pathogenesis for CUD-induced-MDD with higher levels of cortisol and BDNF compared with CUD-primary-MDD. Such variations should imply different approaches in treatment.
A Novel and More Efficient Oscillating Foil for Wave-Driven Unmanned Surface Vehicles
Authors: Gao Yan , Xie Lvcheng , Lam Tin Lun
Published: March 2022

In the wave-driven unmanned surface vehicles (WUSVs), oscillating-foils are the most straightforward and widely used wave energy conversion mechanism, like the wave glider. However, WUSVs usually sail slowly compared with other types of USVs. Improving the thrust of the oscillating foil to increase its speed can help WUSVs improve their maneuverability and shorten the completion of ocean missions. This paper proposed a novel method to enhance oscillating foils’ thrust force using asymmetric cross-section shape and asymmetric oscillating motion. The thrust enhancement effect is verified by CFD simulation and pool experiment. The experimental results show that the asymmetric wing can enhance the propulsive force by at least 13.75%. The speed enhancement of WUSVs brought by this enhanced thrust is at least 7.6%, which has also been verified by simulation and sea experiment. The asymmetric foil only needs to make low-cost modifications on the traditional rigid symmetric foil to achieve the desired thrust enhancement effect.
Global Trends of Forest Loss Due to Fire From 2001 to 2019
Authors: Tyukavina Alexandra , Potapov Peter , Hansen Matthew C. , Pickens Amy H. , Stehman Stephen V. , Turubanova Svetlana , Parker Diana , Zalles Viviana , Lima André , Kommareddy Indrani , Song Xiao-Peng , Wang Lei , Harris Nancy
Published: March 2022

Forest fires contribute to global greenhouse gas emissions and can negatively affect public health, economic activity, and provision of ecosystem services. In boreal forests, fires are a part of the ecosystem dynamics, while in the humid tropics, fires are largely human-induced and lead to forest degradation. Studies have shown changing fire dynamics across the globe due to both climate and land use change. However, global trends in fire-related forest loss remain uncertain due to the lack of a globally consistent methodology applied to high spatial resolution data. Here, we create the first global 30-m resolution satellite-based map of annual forest loss due to fire. When producing this map, we match the mapped area of forest loss due to fire to the reference area obtained using a sample-based unbiased estimator, thus enabling map-based area reporting and trend analysis. We find an increasing global trend in forest loss due to fire from 2001 to 2019, driven by near-uniform increases across the tropics, subtropical, and temperate Australia, and boreal Eurasia. The results quantify the increasing threat of fires to remaining forests globally and may improve modeling of future forest fire loss rates under various climate change and development scenarios.
Case Report: Diagnosis of Primary Klebsiella pneumoniae in Cervical Spine by Metagenomic Next-Generation Sequencing
Authors: Li Tao , Gao Qile , Guo Chaofeng , Li Yanbing
Published: March 2022

Introduction

Spinal infection is a disease that affects the intervertebral disks or adjacent paravertebral tissue in the vertebral body. There are few reports of spinal infections caused by Klebsiella pneumoniae. Cervical spine infection by K. pneumoniae especially preoperative is extremely rare. Nowadays, metagenomic next-generation sequencing (mNGS) has led to the accurate and timely diagnoses of numerous infectious diseases.

Case Presentation

We described a case of a 64-year-old woman, with a chief complaint of neck, shoulder, and upper limb pain for 10 days. The patient had symptoms of abscess compression before surgery, and inflammatory indicators such as erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and procalcitonin (PCT) were significantly elevated. The patient's imaging suggested cervical infectious lesions, and the patient had no symptoms of tuberculosis poisoning, and the blood samples associated with tuberculosis were negative. The patient was diagnosed with cervical suppurative infection before surgery. For the patient who failed conservative treatment and had abscess compression, we performed anterior cervical surgery to remove the lesion at an early stage and collected intraoperative specimens for culture and mNGS. Postoperative antibiotic treatment was adjusted according to the etiology and drug sensitivity.

Conclusion

This case suggests that the clinical symptoms of K. pneumoniae infection are not typical and the imaging examination lacks specificity. When the clinical diagnosis of etiology is not clear or there are symptoms such as abscess compression, early surgical specimens can be collected for culture and mNGS to identify the pathogen, and postoperative sensitive antibiotics can be used to continue treatment. This helps to identify the cause as early as possible, treat it effectively early, relieve symptoms, prevent complications, and keep the spine stable.
Long COVID Through a Public Health Lens: An Umbrella Review
Authors: Nittas Vasileios , Gao Manqi , West Erin A. , Ballouz Tala , Menges Dominik , Wulf Hanson Sarah , Puhan Milo Alan
Published: March 2022

Objectives: To synthesize existing evidence on prevalence as well as clinical and socio-economic aspects of Long COVID.

Methods: An umbrella review of reviews and a targeted evidence synthesis of their primary studies, including searches in four electronic databases, reference lists of included reviews, as well as related article lists of relevant publications.

Results: Synthesis included 23 reviews and 102 primary studies. Prevalence estimates ranged from 7.5% to 41% in non-hospitalized adults, 2.3%–53% in mixed adult samples, 37.6% in hospitalized adults, and 2%–3.5% in primarily non-hospitalized children. Preliminary evidence suggests that female sex, age, comorbidities, the severity of acute disease, and obesity are associated with Long COVID. Almost 50% of primary studies reported some degree of Long COVID-related social and family-life impairment, long absence periods off work, adjusted workloads, and loss of employment.

Conclusion: Long COVID will likely have a substantial public health impact. Current evidence is still heterogeneous and incomplete. To fully understand Long COVID, well-designed prospective studies with representative samples will be essential.
Development of Variable Viscoelastic Joint Module Performance Evaluation and Proposal of Application Examples
Authors: Fujita Tetsuhito , Shimoda Yusuke , Machida Katsuki , Okui Manabu , Nishihama Rie , Nakamura Taro
Published: March 2022

With the diversification of robots, modularization of robots has been attracting attention. In our previous study, we developed a robot that mimics the principle of human joint drive using a straight-fiber-type pneumatic rubber artificial muscle (“artificial muscle”) and a magnetorheological fluid brake (“MR brake”). The variable viscoelastic joints have been modularized. Therefore, this paper evaluates the basic characteristics of the developed Joint Module, characterizes the variable viscoelastic joint, and compares it with existing modules. As basic characteristics, we confirmed that the Joint Module has a variable viscoelastic element by experimentally verifying the joint angle, stiffness, viscosity, and tracking performance of the generated torque to the command value. As a characteristic evaluation, we verified the change in motion and response to external disturbances due to differences in driving methods through simulations and experiments and proved the strength of the variable viscoelastic joint against external disturbances, which is a characteristic of variable viscoelastic joints. Based on the results of the basic characterization and the characterization of the variable viscoelastic drive joint, we discussed what kind of device the Joint Module is suitable to be applied to and clarified the position of the variable viscoelastic joint as an actuator system.
Stay at Home Order—Psychological Stress in Children, Adolescents, and Parents during COVID-19 Quarantine—Data of the CoCo-Fakt Cohort Study, Cologne
Authors: Nöthig Wanja , Klee Lisa , Fabrice Alisa , Eisenburger Nina , Feddern Sven , Kossow Annelene , Niessen Johannes , Schmidt Nikola , Wiesmüller Gerhard A. , Grüne Barbara , Joisten Christine ,   on behalf of the CoCo-Fakt-Group
Published: March 2022

Measures taken to contain the COVID-19 pandemic are particularly stressful for families. Limited data is available regarding the effects of a mandatory quarantine on the psychological stress of children, adolescents and their parents. Quarantined individuals participating in the online-based CoCo-Fakt study had at least one child <3, 3 to <6, 6 to <10, 10 to <14 and 14 to <16 years old (n = 2153). Parents were asked about how often their children felt nervous, anxious, or tense, down or depressed, lonely or physical reactions occur. A relative sum score characterizing psychosocial stress was determined and related to parents’ socio-demographic factors, psychosocial distress, coping strategies and resilience. Parents reported significantly higher psychological stress if at least one child was quarantined. Parents’ relative psychological stress sum score had the strongest influence on the psychological state of the children across all age groups (β = 0.315–0.457) besides male sex of the reporting parent, no partnership, low to medium socioeconomic status, lower resilience and coping scores, and parents quarantined as close contacts. The variance in the linear regression models was between 17.8% and 31.4%. These findings highlight that the entire family system must be considered during official mandatory quarantines.
Modelling the Hydrology of an Upland Catchment of Bystra River in 2050 Climate Using RCP 4.5 and RCP 8.5 Emission Scenario Forecasts
Authors: Badora Damian , Wawer Rafal , Nierobca Anna , Krol-Badziak Aleksandra , Kozyra Jerzy , Jurga Beata , Nowocien Eugeniusz
Published: March 2022

This article presents selected flow modeling indices of the Bystra River catchment area (east Poland) obtained using the SWAT model simulations for three regional climate models driven by the EC-EARTH global climate model for 2021–2050 and both RCP4.5 and RCP 8.5 scenarios. The research area was selected due to the large relief of the terrain, the predominance of soils made of loess and the agricultural nature of the Bystra River catchment area, which is very sensitive to climate change, has very valuable soils, and can be used as a test area for modeling land use-based adaptation measures to climate change. The calibration and validation using the SUFI-2 algorithm in the SWAT CUP program was carried out in order to determine the water balance. After obtaining satisfactory results, the SWAT-CUP program simulated the best parameter values for climate change projections. In analyzed climate projections, the monthly mean sums of actual evapotranspiration and potential evapotranspiration will be higher compared to the simulation period of the 2010–2017 model. The exception is the month of June, where actual evapotranspiration in most climate projections is lower compared to the years 2010–2017. The average monthly total runoff for the Bystra River basin will be lower in most of the 2021–2030 climate change projections for most months compared to the reference period. Also, in the 2031–2040 and 2041–2050 periods, the average monthly total runoff will be lower for the RCP 4.5 scenarios (except for one RCP 4.5 scenario in 2031–2040). Additionally, in the case of the RCP 8.5 for the two scenarios in 2041–2050, the average monthly total runoff will be higher compared to the reference years. We determine that the analysis impact of climate change will result in 31 recognized and different small sub-catchments of the Bystra River, which result from higher precipitation and less evapotranspiration for RCP 8.5 in 2041–2050. All of the above changes in the individual components of the water balance may have a negative impact on the vegetation in the coming decades. The temperature increase and the variable amount of precipitation in individual months may lead to an increased number of extreme phenomena. Increased mean monthly sum of actual and potential evapotranspiration, as well as changes in monthly sums of total runoff, may disturb the vegetation in the studied area at every stage of growth. The above components may also influence changes in the amount of water in the soil (especially during the growing season). Counteracting the effects of future climate change requires various adaptation measures.
Parameter Optimization of Newly Developed Self-Propelled Variable Height Crop Sprayer Using Response Surface Methodology (RSM) Approach
Authors: Khan Fraz Ahmad , Ghafoor Abdul , Khan Muhammad Azam , Umer Chattha Muhammad , Kouhanestani Farzaneh Khorsandi
Published: March 2022

The number of spray deposits plays an important role in effective and efficient spraying. The spraying equipment is one of the most significant factors that affect the number of spray deposits. Therefore, the study was focused on the parameter optimization of a newly developed self-propelled variable height crop sprayer. Response surface methodology (RSM) along with Box–Behnken design (BBD) was used to study the effect of the independent variables (forward speed, spray height, and spray pressure) on response variables such as droplet density, coverage per-centage, and Volume Median Diameter (VMD). The experiment was conducted in the cotton field. Additionally, the RSM model was validated in this research. The results revealed that the coefficient of determination (R2) values was good for all response variables in the quadratic polynomial model. The optimized parameters were 6.5 km/h, 60 cm, 4 bar for fungicide application, and 8 km/h, 70 cm, 3 bar for insecticide and herbicide application. The predicted response variable values at the optimal conditions were 60.4 droplet/cm2, 27%, 230 µm for fungicides and 37.8 droplet/cm2, 19.1%, 225.4 µm for insecticide and herbicides application. The model validation is confirmed by the mean of actual response variable values at the optimal condition for insecticide and herbicides application, which was 41.35 ± 3.67 droplet/cm2, 21.10 ± 1.72%, 227.43 ± 1.22 µm, and the prediction error was 8.46%, 9.2%, and 0.9% for droplet density, coverage percentage, and VMD, respectively. This study can provide support for further optimizing the parameters of the sprayer.
First Report of Benzoxazinoid Compounds in Woolly Cupgrass (Eriochloa villosa Thunb. Kunth), an Invasive Plant
Authors: Makleit Peter , Szilágyi Arnold , Veres Szilvia
Published: March 2022

Woolly cupgrass (Eriochloa villosa Thunb. Knuth) is a common weed which is native to East Asia. It also was introduced into North America and Europe. The problems related to controlling this annual weed are due to its germination characteristics, low susceptibility to certain herbicides and considerable competitiveness against other plant species. Several genera of the Panicoideae subfamily contain benzoxazinoids, therefore we hypothesized that woolly cupgrass also produces these chemicals. To test this hypothesis, the benzoxazinoid content of plants at the flowering stage was investigated. Our results demonstrate the presence of benzoxaziniods in woolly cupgrass and this represents the first report of these compounds in this species within the Poaceae family and the Panicoideae subfamily. Benzoxazinoids likely account for the rapid spread and ecological success of woolly cupgrass in its invasive range.
Awareness of Antimicrobial Resistance and Associated Factors among Layer Poultry Farmers in Zambia: Implications for Surveillance and Antimicrobial Stewardship Programs
Authors: Mudenda Steward , Malama Sydney , Munyeme Musso , Hang’ombe Bernard Mudenda , Mainda Geoffrey , Kapona Otridah , Mukosha Moses , Yamba Kaunda , Bumbangi Flavien Nsoni , Mfune Ruth Lindizyani , Daka Victor , Mwenya Darlington , Mpundu Prudence , Siluchali Godfrey , Muma John Bwalya
Published: March 2022

Antimicrobial resistance (AMR) is a global public health problem affecting animal and human medicine. Poultry production is among the primary sources of income for many Zambians. However, the increased demand for poultry products has led to a subsequent increase in antimicrobial use. This study assessed the awareness of AMR and associated factors among layer poultry farmers in Zambia. A cross-sectional study was conducted among 77 participants from September 2020 to April 2021. Data was analysed using Stata version 16.1. The overall awareness of AMR among the farmers was 47% (n = 36). The usage of antibiotics in layer poultry production was high at 86% (n = 66). Most antibiotics were accessed from agrovets (31%, n = 24) and pharmacies (21%, n = 16) without prescriptions. Commercial farmers were more likely to be aware of AMR compared to medium-scale farmers (OR = 14.07, 95% CI: 2.09–94.70), as were farmers who used prescriptions to access antibiotics compared to those who did not (OR = 99.66, 95% CI: 7.14–1391.65), and farmers who did not treat market-ready birds with antibiotics compared to those who did (OR = 41.92, 95% CI: 1.26–1396.36). The awareness of AMR among some layer farmers was low. Therefore, policies that promote the rational use of antibiotics need to be implemented together with heightened surveillance activities aimed at curbing AMR.
Prevalence and Antibiotic Susceptibility Trends of Selected Enterobacteriaceae, Enterococci, and Candida albicans in the Subgingival Microbiota of German Periodontitis Patients: A Retrospective Surveillance Study
Authors: Jepsen Karin , Falk Wolfgang , Brune Friederike , Cosgarea Raluca , Fimmers Rolf , Bekeredjian-Ding Isabelle , Jepsen Søren
Published: March 2022

The periodontal microbiota is ecologically diverse and may facilitate colonization by bacteria of enteric origin (Enterobacteriaceae, Enterococci) and co-infections with Candida albicans, possibly producing subgingival biofilms with high antimicrobial tolerance. This retrospective surveillance study followed periodontitis-associated superinfection profiles in a large patient sample. From 2008 to 2015, biofilm samples from deep periodontal pockets were collected from a total of 16,612 German adults diagnosed with periodontitis. The presence of selected Enterobacteriaceae, Enterococci, and Candida albicans was confirmed in overnight cultures. Antimicrobial susceptibility of these clinical isolates was tested by disk diffusion with antibiotics routinely used for treatment of oral infections, e.g., amoxicillin (AML), amoxicillin/clavulanic acid (AMC), doxycycline (DO), and ciprofloxacin (CIP). The mean annual prevalence of patients harboring Enterobacteriaceae in periodontal plaques was 11.5% in total and ranged from 2.5% for Enterobacter cloacae to 3.6% for Klebsiella oxytoca, 1.1% for Klebsiella pneumoniae, 2.8% for Serratia marcescens, and 1.5% for Serratia liquefaciens. In comparison, the mean detection rates for microbiota typically found in the oral cavity were higher, e.g., 5.6% for Enterococcus spp. and 21.8% for Candida albicans. Among the Enterobacteriaceae, species harboring intrinsic resistance to AML (Enterobacter spp., Klebsiella spp., Serratia spp.) were predominant. Non-susceptibility to AMC was observed for Serratia spp. and Enterobacter cloacae. By contrast, Enterococcus spp. only showed non-susceptibility to DO and CIP. Trends for increasing resistance were found to AML in Serratia liquefaciens and to DO in Enterococcus spp. Trend analysis showed decreasing resistance to AMC in Serratia liquefaciens and Klebsiella oxytoca; and to DO in Serratia marcescens, liquefaciens, and Enterobacter cloacae. This study confirms the low but consistent presence of Enterobacteriaceae and Enterococci among the subgingival microbiota recovered from periodontitis specimen. Although their pathogenetic role in periodontal lesions remains unclear, their presence in the oral cavity should be recognized as a potential reservoir for development and spread of antibiotic resistance in light of antibiotic usage in oral infections.