bluuebunny/crossref_metadata_2025 huggingface dataset:
Testing New Provenances of Eucalyptus polybractea: A Eucalypt Oil Mallee Adapted to Semi-Arid Environments
Authors: Bush David , Spencer David , Doran John , Davis Richard
Published: July 2022

Novel genetic accessions of Eucalyptus polybractea from a previously untested, hotter and drier part of the species’ natural range were tested in a common garden trial at a semi-arid site in NSW, Australia. Eucalyptus polybractea is a mallee eucalypt cultivated for essential oils (1,8-cineole), bioenergy and carbon sequestration on dryland sites in southern Australia (sites receiving about 450 mm mean annual rainfall, MAR). A trial of six previously untested provenances from the relatively hot, dry part of the species’ natural range in South Australia (SA) (250–450 mm MAR) was established alongside seven provenances from New South Wales (NSW) and Victoria within a commercial plantation in NSW. The trial was assessed at age 3.7 years for growth and oil characteristics. While survival was excellent, most of the SA sources were slower growing and of sub-standard oil concentration and quality relative to those from Victoria and NSW. However, a single SA provenance, with the highest oil concentration and 1,8-cineole percentage of all provenances tested, may have potential as a source of selected germplasm. Infusion of SA material into the breeding populations of E. polybractea, which are currently based on NSW and Victorian selections only, may provide more resilience in the face of hotter and drier temperatures expected under projected climate change scenarios, and/or allow the introduction of the species to hotter and drier climates in Australia or other parts of the world with semi-arid climates. However, high-intensity selection of infusions will be required to maintain the growth and oil characteristics in the existing breeding population.
Soil Microbial and Organic Carbon Legacies of Pre-Existing Plants Drive Pioneer Tree Growth during Subalpine Forest Succession
Authors: Liang Ting , Zhao Wenqiang , Kou Yongping , Liu Jia , Liu Qing
Published: July 2022

Fast-growing pioneer tree species play a crucial role in triggering late successional development in forests. Experimental evidence of the soil legacy effects of pre-existing plants on pioneer tree performance is lacking. We explored the legacy effects of soils conditioned by early successional herbs (Poa poophagorum Bor and Potentilla fragarioides L.) and mid-successional shrubs (Rhododendron fortunei Lindl. and Enkianthus quinqueflorus Lour.) on late-successional ectomycorrhizal (ECM) pioneer tree (Betula platyphylla Sukaczev) seedling growth. The soils were analyzed for soil nutrient status and fungal and bacterial compositions using ITS and 16S rRNA gene sequencing. B. platyphylla seedlings produced higher biomass in soils conditioned by shrubs. Soil organic carbon (SOC) and bacterial and fungal legacies most impacted pioneer tree seedling growth. Additionally, the partial least squares path model revealed that soil nutrients, especially SOC, indirectly affected seedling biomass by their direct effects on the bacterial and fungal communities. The changes in bacterial community composition had a stronger effect on seedling biomass than those of fungi because bacteria with shorter turnover times are generally considered to be more efficient than fungi in enhancing nutrient availability. Our study integrates soil microbial and nutrient legacies to explain the potential mechanisms of pioneer tree regeneration.
The Relationship between Breast Height Form Factor and Form Quotient of Liquidambar formosana in the Eastern Part of Taiwan
Authors: Peng Ping-Hsun , Kuo Chia-Ho , Wei Chun-Hung , Hsieh Yi-Ta , Chen Jan-Chang
Published: July 2022

The breast height form factor and quotient of trees are important parameters for calculating the stumpage volume in forest management. However, many types of datasets can only be accumulated by cutting down many trees. This research studied different thinning intensities of Formosan sweet gum (Liquidambar formosana) in the Danongdafu Forest Park, Guangfu Township, Hualien County, Taiwan. The relationship and transformation modes of the breast height form factor (F1.3) and the normal form quotient (Qn) were examined using a nondestructive terrestrial laser scanning (TLS) system, which can replace the conventional method of obtaining F1.3. The results demonstrated that the average F1.3 and Qn in the low-intensity thinning area were 0.40 and 0.56, respectively. The average F1.3 and Qn in the high-intensity thinning area were 0.48 and 0.68, respectively. Whether the stand density was low- or high-intensity could be determined statistically depending on the optimal results of F1.3. There was one mode of estimation when the stand density was 1200 trees ha−1 and another when it was 600 trees ha−1. The root mean square error of the equations used was 0.002, and the mean absolute error was <0.02. The mean percentage error was ±2.3%, and the mean absolute percentage error was <5%. If the TLS system can obtain datasets of quotient and height with surveys in the future, this would enable the calculation of F1.3. The stock volume would be calculated precisely, and the calculation error would be improved.
An Integrative Approach to Study How Driving Factors Control Biomass Carbon Density for Natural Mountain Forests, in China’s Loess Plateau
Authors: Sun Lina , Wang Qixiang , Fan Xiaohui
Published: July 2022

Mountain forests, accounting for 84.95% of the total forest area, are the most important part of the natural vegetation in China. An assessment of the factors affecting the carbon capture capacity of mountain forests is very crucial to realizing the nation’s goal of capping carbon-emissions growth by 2030. Based on the 9th national forest inventory data in the eastern Loess Plateau of China, which is mountainous terrain, we characterized the spatial pattern of biomass carbon density (BCD) for natural coniferous and broad-leaved forests using Local Getis-ord G* and proposed an integrative framework to evaluate the direct and indirect effects of stand, geographical and climatic factors on BCD for the two types of forests using structural equation modeling. The results showed that there was no significant difference between the mean BCDs of the natural coniferous and broad-leaved forests. Compared with broad-leaved forests, the hot spots of BCDs at the 1% significance level for coniferous forests were located in areas with higher average latitude, higher average elevation, lower mean temperature, or lower mean precipitation. Stand age and elevation were important driving factors, which had stronger effects for the coniferous forests than broad-leaved forests. Among all driving factors, age had the strongest total effect for the two forests types. No significant difference was detected in BCDs between natural coniferous and broad-leaved forests. Spatial patterns of BCDs were different between the two forests types. Stand age and elevation were important driving factors, which had stronger effects for the coniferous forests than broad-leaved forests.
Development of a Robust Machine Learning Model to Monitor the Operational Performance of Fixed-Post Multi-Blade Vertical Sawing Machines
Authors: Borz Stelian Alexandru , Forkuo Gabriel Osei , Oprea-Sorescu Octavian , Proto Andrea Rosario
Published: July 2022

Monitoring the operational performance of the sawmilling industry has become important for many applications including strategic and tactical planning. Small-scale sawmilling facilities do not hold automatic production management capabilities mainly due to using obsolete technology which is an effect of low financial capacity and focus their strategy on increasing value recovery and saving resources and energy. Based on triaxial acceleration data collected over five days at a sampling rate of 1 Hz, a robust machine learning model was developed with the purpose of using it to infer the operational events based on lower sampling rates adopted as a strategy to collect long-term data. Among its performance metrics, the model was characterized in its training phase by a very high overall classification accuracy (CA = 98.7%), F1 score (98.4%) and a very low error rate (LOG LOSS = 5.6%). For a three-class problem, it worked very well in classifying the main events related to the operation of the machine, with active work being characterized by an F1 score of 99.6% and an error of 3.6%. By accounting for the same metrics, the model was proven to be invariant to the sampling rates of up to 0.05 Hz (20 s) and produced even better results in the testing phase (CA = 98.9%, F1 = 98.6%, LOG LOSS = 5.5%, for a testing sample extracted at 0.05 Hz), while there were no differences in the share of class data irrespective of the sampling rate. The developed model not only preserves a high classification performance in the training and testing phases but it also seems to be invariant to lower sampling rates, making it useful for prediction over data collected at low sampling rates. In turn, this would enable the use of cheap data collectors to be operated for extended periods of time in various locations and will save human resources and money associated with data collection. Further tests would be required only for validation and they could be supported by collecting and feeding new data to the model to infer the long-term performance of similar sawmilling machines.
Dissolved Organic Carbon Flux Is Driven by Plant Traits More Than Climate across Global Forest Types
Authors: Ji Yuhuang , He Yanghui , Shao Junjiong , Liu Huiying , Fu Yuling , Chen Xinyue , Chen Yang , Liu Ruiqiang , Gao Jing , Li Nan , Zhou Guiyao , Zhou Lingyan , Zhou Xuhui
Published: July 2022

Dissolved organic carbon (DOC) is one of the most important components in the global carbon cycle, which is largely influenced by climate and plant traits. Although previous studies have examined the impacts of climatic factors (e.g., mean annual temperature (MAT) and precipitation (MAP)) or plant traits (e.g., leaf area index, leaf nitrogen) on DOC, the relative importance of climate and plant traits on DOC flux remains unclear on a global scale. In this study, we compiled 153 pairs of DOC observational data from 84 forest sites to explore the relative importance of climate and plant traits on DOC flux with a linear mixed model, variance partitioning, and random forest approaches. Our results showed that DOC fluxes from throughfall and the litter layer were higher in broadleaved forests than those in coniferous forests. Throughfall-DOC flux increased significantly with MAT and MAP in coniferous forests, but that from the litter layer showed no significant correlations with climate factors. In broadleaved forests, throughfall-DOC flux increased with potential evapotranspiration (PET), while that from the litter layer was positively correlated with MAT. Meanwhile, throughfall-DOC flux had negative relationships with specific leaf area (SLA), leaf nitrogen content (LN), and leaf phosphorus content (LP) in broadleaved forests, but it showed a positive correlation with SLA in coniferous forests. Litter-layer-DOC flux increased with LN in broadleaved forests, but this correlation was the opposite in coniferous forests. Using the variance partitioning approach, plant traits contributed to 29.0% and 76.4% of the variation of DOC from throughfall and litter layer, respectively, whereas climate only explained 19.1% and 8.3%, respectively. These results indicate that there is a more important contribution by plant traits than by climate in driving the spatial variability of global forest DOC flux, which may help enhance forest management as a terrestrial carbon sink in the future. Our findings suggest the necessity of incorporating plant traits into land surface models for improving predictions regarding the forest carbon cycle.
Compression of Acetylated Beech (Fagus sylvatica L.) Laminated Veneer Lumber (LVL)
Authors: Slabohm Maik , Mayer Aaron Kilian , Militz Holger
Published: July 2022

Acetylation with acetic anhydride is well known to improve the dimensional stability and durability of wood. Veneer is appealing for acetylation because of its thin thickness, which supports a complete and even impregnation of difficult-to-treat wood species, such as beech (Fagus sylvatica L.). Unlike resin-based veneer impregnation, acetylated veneer does not require any additional curing. As a result, veneer properties are already altered prior to bonding. The compression thickness reduction in acetylated beech veneer during the manufacturing of laminated veneer lumber (LVL) utilizing 1, 3, and 6 MPa at 150 °C for 30 min is investigated in this study. The results show that acetylated beech veneer is considerably less compressible than the references. Moreover, the density of acetylated LVL at low pressure (1 MPa) is similar to the one of references, even though the compressibility is much lower. This is due to the added acetyl groups after acetylation. The reduction in compressibility is most likely caused due to a decrease in moisture content (MC) and its accompanied mechanisms.
Improvements in Human Keratinocytes and Antimicrobial Effect Mediated by Cell-Free Supernatants Derived from Probiotics
Authors: Lee Ji Yeon , Kim YongGyeong , Kim Ja-I , Lee Hyang-Yeol , Moon Gi-Seong , Kang Chang-Ho
Published: July 2022

The skin acts as a physical and physiological barrier, thereby protecting the body from various environmental components and stimuli. Cell-free supernatants (CFS) derived from probiotics can improve skin functions and retain moisture. In this study, to assess the efficacy of CFS derived from Ligilactobacillus salivarius and Limosilactobacillus fermentum, we investigated the barrier strengthening and moisturizing effects of CFS in keratinocytes along with their antibacterial effects. We also determined the adhesive effects of probiotics on colorectal cells. To confirm improvements in moisturization and barrier function mediated by CFS in keratinocytes, hyaluronic acid (HA) production, and mRNA expression of HA synthases (HAS)2, HAS3, and FLG were measured. The results showed that CFS from L. salivarius MG242 and L. fermentum MG901 increased the expression of these genes along with the production of HA (2.40- and 1.95-fold of control). Additionally, CFS derived from L. salivarius MG242 and L. fermentum MG901 inhibited the growth of S. aureus and E. coli, thereby demonstrating inhibitory effects against harmful pathogens observed on the skin. These results indicate that the use of CFS derived from L. salivarius MG242 and L. fermentum MG901 may increase moisturization in the skin and improve barrier function of keratinocytes along with elimination of potential pathogens.
Increase in Electrical Parameters Using Sucrose in Tomato Waste
Authors: Segundo Rojas-Flores , Magaly De La Cruz-Noriega , Benites Santiago M. , Daniel Delfín-Narciso , Angelats-Silva Luis , Díaz Felix , Luis Cabanillas-Chirinos , Fernanda Silva-Palacios
Published: July 2022

The use of organic waste as fuel for energy generation will reduce the great environmental problems currently caused by the consumption of fossil sources, giving agribusiness companies a profitable way to use their waste. In this research, tomato waste with different percentages of sucrose (0-target, 5, 10, and 20%) was used in microbial fuel cells manufactured on a laboratory scale with zinc and copper electrodes, managing to generate maximum peaks of voltage and a current of 1.08 V and 6.67 mA in the cell with 20% sucrose, in which it was observed that the optimum operating pH was 5.29, while the MFC with 0% (target) sucrose generated 0.91 V and 3.12 A on day 13 with a similar pH, even though all the cells worked in an acidic pH. Likewise, the cell with 20% sucrose had the lowest internal resistance (0.148541 ± 0.012361 KΩ) and the highest power density (224.77 mW/cm2) at a current density of 4.43 mA/cm2, while the MFC with 0% sucrose generated 160.52 mW/cm2 and 4.38 mA/cm2 of power density and current density, respectively, with an internal resistance of 0.34116 ± 0.2914 KΩ. In this sense, the FTIR (Fourier-transform infrared spectroscopy) of all the substrates used showed a high content of phenolic compounds and carboxylate acids. Finally, the MFCs were connected in a series and managed to generate a voltage of 3.43 V, enough to light an LED (green). These results give great hope to companies and society that, in the near future, this technology can be taken to a larger scale.
Bioprotective Effect of a Torulaspora delbrueckii/Lachancea thermotolerans-Mixed Inoculum in Red Winemaking
Authors: Escribano-Viana Rocío , González-Arenzana Lucía , Garijo Patrocinio , Fernández Laura , López Rosa , Santamaría Pilar , Gutiérrez Ana Rosa
Published: July 2022

One of the alternatives to SO2 as an antimicrobial is the use of bioprotection yeasts, which colonize the medium preventing the proliferation of undesirable microorganisms. In this work, the bioprotective effect of a mixed inoculum formed by Torulaspora delbrueckii/Lachancea thermotolerans during fermentation was evaluated. For this purpose, fermentations were carried out using this mixed inoculum and the populations of yeasts, lactic bacteria and acetic bacteria, and the physical–chemical parameters of the wines obtained were studied. The results were compared with those obtained in spontaneous fermentation with and without SO2. The different fermentation strategies caused a differentiation in the yeast species present during fermentation. Regarding populations of lactic acid bacteria, results showed that the effect of the addition of the mixed inoculum was comparable to that exerted by SO2. On the other hand, due to the high sensitivity of acetic acid bacteria to SO2, the sulfite vinifications showed a lower population of acetic acid bacteria in the early stages of fermentation, followed by the vinifications with the mixed inoculum.
The Hot-Water Extract of Sargassum sp. as a Feed Ingredient for Spotted Scat (Scatophagus argus Linnaeus, 1766) Reared in Songkhla Lake: Effects on Growth, Feed Efficiency, Hematological Data and Body Composition
Authors: Yangthong Monsuang , Ruensirikul Jirayuth , Kaneko Gen
Published: July 2022

The growth performance of Scatophagus argus (25.53 ± 0.89 g) reared in Songkhla Lake, a brackish lagoon, was evaluated after feeding isonitrogenous and isocaloric diets containing hot-water extract from a seaweed, Sargassum sp., at 0, 0.25, 0.50, 0.75 or 1 g·kg−1. Triplicate groups of fish were reared in 15 net cages (50 × 50 × 60 cm) at a density of 20 fish per cage, and the diets were fed twice a day at a rate of 5% of fish body weight per day for 8 weeks. The final body weight, weight gain (WG), average daily gain, and feed intake (FI) showed significant positive and quadratic responses to the Sargassum extract level. The highest WG (57.97%) and FI (0.34) were recorded in the fish that received the hot-water extract at 0.25–0.5 g·kg−1 of diet. The protein efficiency ratio showed significant positive linear and quadratic responses to the Sargassum extract level, while the hepatosomatic index showed a significant positive linear response to the Sargassum extract level. The body protein and ash contents showed positive, linear and quadratic responses to the Sargassum extract level, and the highest crude protein (49%) was observed in the 0.5 g·kg−1 diet group. There were no significant effects of the Sargassum extract levels on feed conversion ratio, food conversion efficiency, lipid and moisture contents, survival rate, hematocrit, and white or red blood cell counts. However, hematological data between fish in the control group and the Sargassum extract groups tended to be different. Overall, the optimum level of seaweed-extract supplementation for spotted scat under natural conditions was determined to be approximately 0.5 g·kg−1 diet.
POD-Based Model-Order Reduction for Discontinuous Parameters
Authors: Karcher Niklas
Published: July 2022

Reduced-order models (ROMs) based on proper orthogonal decomposition (POD) are widely used in industry. Due to the rigid requirements on the input data, these methods struggle with discontinuous parameters, e.g., optional rear spoiler on a car. In order to also include these types of parameters, a new method is presented that splits the full-order model (FOM) domain with its discontinuous parameters into multiple ROM subdomains. The resulting subdomains then again comply with the ROM requirements, and the established and proven ROM methods can be applied. The steps involved in computing a ROM based on the proposed method, by setting up the subdomains, mapping the FOM data into the domains, as well as computing the ROMs on the domains, are shown in detail in this paper. The method is employed on two use cases. The academic one-dimensional use case focuses on how the steps involved are employed and analyzes the introduced errors. The second use case’s FOM is based on the DrivAer body with an optional rear spoiler computed using computational fluid dynamics (CFD) and demonstrates the usage in an industrial environment.
CHP-Based Economic Emission Dispatch of Microgrid Using Harris Hawks Optimization
Authors: Tiwari Vimal , Dubey Hari Mohan , Pandit Manjaree , Salkuti Surender Reddy
Published: July 2022

In this paper, the economically self-sufficient microgrid is planned to provide electric power and heat demand. The combined heat and power-based microgrid needs strategic placement of distributed generators concerning optimal size, location, and type. As fossil fuel cost and emission depend mainly on the types of distributed generator units used in the microgrid, economic emission dispatch is performed for an hour with a static load demand and multiple load demands over 24 h of a day. The TOPSIS ranking approach is used as a tool to obtain the best compromise solution. Harris Hawks Optimization (HHO) is used to solve the problem. For validation, the obtained results in terms of cost, emission, and heat are compared with the reported results by DE and PSO, which shows the superiority of HHO over them. The impact of renewable integration in terms of cost and emission is also investigated. With renewable energy integration, fuel cost is reduced by 18% and emission is reduced by 3.4% for analysis under static load demand, whereas for the multiple load demands over 24 h, fuel cost is reduced by 14.95% and emission is reduced by 5.58%.
A Unified Inertial Iterative Approach for General Quasi Variational Inequality with Application
Authors: Akram Mohammad , Dilshad Mohammad
Published: July 2022

In this paper, we design two inertial iterative methods involving one and two inertial steps for investigating a general quasi-variational inequality in a real Hilbert space. We establish an existence result and a non-trivial example is furnished to substantiate our theoretical findings. We discuss the convergence of the inertial iterative algorithms to approximate the solution of a general quasi-variational inequality. Finally, we apply an inertial iterative scheme with two inertial steps to investigate a delay differential equation. The results presented herein can be seen as substantial generalizations of some known results.
Measuring, Mapping, and Evaluating Daytime Traffic Noise Levels at Urban Road Intersections in Doha, Qatar
Authors: Abdur-Rouf Khadija , Shaaban Khaled
Published: July 2022

In this study, equivalent hourly traffic noise levels at different intersections in the city of Doha, Qatar were measured and compared to the local and World Health Organization (WHO) thresholds. As part of the study, equivalent sound pressure levels, ambient temperature, humidity, and wind speed were recorded during the morning, afternoon, and evening hours on weekdays and weekends. The results showed that regardless of the day (weekday or weekend), the mean 16-h daytime traffic noise levels at all sites exceeded the local and the WHO’s recommended thresholds. The values of the mean weekday noise levels at the sites ranged between 67.6 dB(A) and 77.5 dB(A), whereas the weekend values ranged between 68.8 dB(A) and 76.9 dB(A). The measured noise levels were also compared with traffic noise levels reported in other countries. Finally, some recommendations to reduce excessive traffic noise levels were suggested. The results of the study could be used as a benchmark of traffic noise levels in the country after the implementation of any countermeasures in the future.
The Intermediate Value Theorem and Decision-Making in Psychology and Economics: An Expositional Consolidation
Authors: Ghosh Aniruddha , Khan Mohammed Ali , Uyanik Metin
Published: July 2022

On taking the intermediate value theorem (IVT) and its converse as a point of departure, this paper connects the intermediate value property (IVP) to the continuity postulate typically assumed in mathematical economics, and to the solvability axiom typically assumed in mathematical psychology. This connection takes the form of four portmanteau theorems, two for functions and the other two for binary relations, that give a synthetic and novel overview of the subject. In supplementation, the paper also surveys the antecedent literature both on the IVT itself, as well as its applications in economic and decision theory. The work underscores how a humble theorem, when viewed in a broad historical frame, bears the weight of many far-reaching consequences; and testifies to a point of view that the apparently complicated can sometimes be under-girded by a most basic and simple execution.
Enhanced Development of Sweat Latent Fingerprints Based on Ag-Loaded CMCS/PVA Composite Hydrogel Film by Electron Beam Radiation
Authors: Yang Jinyu , Wang Yayang , Zhao Yuan , Liu Dongliang , Rao Lu , Wang Zhijun , Fu Lili , Wang Yifan , Yang Xiaojie , Li Yuesheng , Liu Yi
Published: July 2022

Over time, difficulties have been encountered in detecting potential fingerprints. In this study, an Ag/CMCS/PVA(ACP) hydrogel film was developed for fingerprint development by electron beam radiation method. The chemical bond, thermostability, chemical components, microstructure, and micromorphology of the CMCS/PVA composite hydrogel film were characterized by FT-IR, TG, XRD, and SEM, respectively. Swelling behaviors and mechanical performance of the CMCS/PVA composite hydrogel were also investigated at different irradiation doses, pH, media, and NaCl contents to obtain the optimum preparation conditions. Through experimental exploration, we found that the fingerprints appeared more obvious when the irradiated prepared ACP hydrogel film was sprayed with 0.6 mg/mL of Ag+ and the excitation wavelength was about 254 nm with UV lamp irradiation for 20 min. The cytotoxicity the CMCS/PVA composite hydrogel on mouse skin fibroblasts L929 cells was also studied, confirming its biological security. Sweat latent fingerprint manifestation has important scientific significance with respect to the development of new processes and functional materials in the field of fingerprint manifestation, enriching and complementing the application of composite hydrogels.
A New Compound Staged Gelling Acid Fracturing Method for Ultra-Deep Horizontal Wells
Authors: Wang Yang , Fan Yu , Wang Tianyu , Ye Jiexiao , Luo Zhifeng
Published: July 2022

Carbonate gas reservoirs in Sichuan are deeply buried, high temperature and strong heterogeneity. Staged acid fracturing is an effective means to improve production. Staged acidizing fracturing of ultra-deep horizontal wells faces the following problems: 1. Strong reservoir heterogeneity leads to the difficulty of fine segmentation; 2. The horizontal well section is long and running too many packers increases the completion risk; 3. Under high temperatures, the reaction speed between acid and rock is rapid and the acid action distance is short; and 4. The fracture conductivity is low under high-closure stress. In view of the above problems, the optimal fracture spacing is determined through productivity simulation. The composite temporary plugging of fibers and particles can increase the plugging layer pressure to 17.9 MPa, which can meet the requirements of the staged acid fracturing of horizontal wells. Through the gelling acid finger characteristic simulation and conductivity test, it is clear that the crosslinked authigenic acid and gelling acid in the Sichuan carbonate gas reservoir are injected alternately in three stages. When the proportion of gelling acid injected into a single section is 75% and the acid strength is 1.6 m3/m, the length and conductivity of acid corrosion fracture are the best. A total of 12 staged acid fracturing horizontal wells have been completed in the Sichuan carbonate gas reservoir, and the production is 2.1 times that of ordinary acid fracturing horizontal wells.
Challenges in Diagnosing Primary Ciliary Dyskinesia in a Brazilian Tertiary Hospital
Authors: Toro Mariana Dalbo Contrera , Ribeiro José Dirceu , Marson Fernando Augusto Lima , Ortiz Érica , Toro Adyléia Aparecida Dalbo Contrera , Bertuzzo Carmen Silvia , Jones Marcus Herbert , Sakano Eulália
Published: July 2022

Primary ciliary dyskinesia (PCD) causes cellular cilia motility alterations, leading to clinical manifestations in the upper and lower respiratory tract and situs abnormalities. The PCD diagnosis was improved after the inclusion of diagnostic tools, such as transmission electron microscopy and genetic screening; however, the PCD screening is a challenge yet. In this context, we aimed to describe the clinical, genetic, and ultra-ciliary characteristics in individuals with clinical suspicion of PCD (cPCD) from a Brazilian Tertiary Hospital. An observational study was carried out with individuals during the follow-up between 2011 and 2021. The individuals were submitted to clinical questionnaires, transmission electron microscopy, and genetic screening for pathogenic variants in PCD-related genes. Those patients were classified according to the degree of suspicion for PCD. In our study, we enrolled thirty-seven cPCD individuals; 20/37 (54.1%) had chronic rhinosinusitis, 28/37 (75.6%) had bronchiectasis, and 29/37 (78.4%) had recurrent pneumonia. A total of 17/37 (45.9%) individuals had transmission electron microscopy or genetic confirmation of PCD; 10 individuals had at least one positive pathogenic genetic variant in the PCD-related genes; however, only seven patients presented a conclusive result according to the American College of Medical Genetics and Genomics and the Association for Molecular Pathology with two pathogenic variants in homozygous or compound heterozygous. The median age at diagnosis was 13 years, and the median time between suspicion and diagnosis was four years. Sixteen patients had class I electron microscopy alterations, seven had class II alterations, and 14 had normal transmission electron microscopy according to the international consensus guideline for reporting transmission electron microscopy results in the diagnosis of PCD (BEAT-PCD TEM Criteria). Genetic screening for pathogenic variants in PCD-related genes and transmission electron microscopy can help determine the PCD diagnosis; however, they are still unavailable to all individuals with clinical suspicion in Brazil. We described ultrastructural alterations found in our population along with the identification of pathogenic variants in PCD-related genes.
Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion
Authors: Sugasawa Takehito , Kanki Yasuharu , Komine Ritsuko , Watanabe Koichi , Takekoshi Kazuhiro
Published: July 2022

The World Anti-Doping Agency (WADA) has prohibited the use of autologous blood transfusion (ABT) as a doping method by athletes. It is difficult to detect this doping method in laboratory tests, and a robust testing method has not yet been established. We conducted an animal experiment and used total RNA sequencing (RNA-Seq) to identify novel RNA markers to detect ABT doping within red blood cells (RBCs) as a pilot study before human trials. This study used whole blood samples from Wistar rats. The whole blood samples were mixed with a citrate–phosphate–dextrose solution with adenine (CPDA) and then stored in a refrigerator at 4 °C for 0 (control), 10, or 20 days. After each storage period, total RNA-Seq and bioinformatics were performed following RNA extraction and the purification of the RBCs. In the results, clear patterns of expression fluctuations were observed depending on the storage period, and it was found that there were large numbers of genes whose expression decreased in the 10- and 20-day periods compared to the control. Moreover, additional bioinformatic analysis identified three significant genes whose expression levels were drastically decreased according to the storage period. These results provide novel insights that may allow future studies to develop a testing method for ABT doping.
The Genetic Variants in the Renin-Angiotensin System and the Risk of Heart Failure in Polish Patients
Authors: Gorący Iwona , Gorący Anna , Kaczmarczyk Mariusz , Rosik Jakub , Lewandowska Klaudyna , Ciechanowicz Andrzej
Published: July 2022

(1) Background: Heart failure (HF) is a complex disease and one of the major causes of morbidity and mortality in the world. The renin-angiotensin system (RAS) may contribute to the pathogenesis of HF. (2) Aim: To investigate the association of RAS key genetic variants, rs5051 (A-6G) in the gene encoding angiotensinogen (AGT), rs4646994 (I/D) in the gene for angiotensin I converting enzyme (ACE), and rs5186 (A1166C) in the gene encoding type 1 receptor for angiotensin II (AGTR1), with the HF risk in the cohort of Polish patients. (3) Methods: The study group consisted of 415 patients that were diagnosed with HF, while the control group comprised of 152 healthy individuals. Genomic DNA were extracted from blood and genotyping was carried out using either PCR or PCR-RFLP for ACE or AGT and AGTR1 variants, respectively. (4) Results: No association has been found between the I/D ACE and heart failure. The HF risk was significantly higher for AG AGT heterozygotes (overdominance: AG versus AA + GG) and for carriers of the G AGT allele in codominant and dominant modes of inheritance. However, the risk of HF was significantly lower in the carriers of at least one C AGTR1 allele (AC or CC genotypes) or in AC AGTR1 heterozygotes (overdominant mode). There was a significant relationship for AGT and HF patients in NYHA Class I-II for whom the risk was higher for the carriers of the G allele, and for the AG heterozygotes. There was also a significant interaction between heterozygote advantage of AGT and BMI increasing the risk for HF. (5) Conclusion: Our results suggest that the A(-6)G AGT polymorphism may be associated with HF in the Polish population and the HF risk seems to be modulated by the A1166C AGTR1 polymorphism.
Functional Genomics Analysis to Disentangle the Role of Genetic Variants in Major Depression
Authors: Pérez-Granado Judith , Piñero Janet , Medina-Rivera Alejandra , Furlong Laura I.
Published: July 2022

Understanding the molecular basis of major depression is critical for identifying new potential biomarkers and drug targets to alleviate its burden on society. Leveraging available GWAS data and functional genomic tools to assess regulatory variation could help explain the role of major depression-associated genetic variants in disease pathogenesis. We have conducted a fine-mapping analysis of genetic variants associated with major depression and applied a pipeline focused on gene expression regulation by using two complementary approaches: cis-eQTL colocalization analysis and alteration of transcription factor binding sites. The fine-mapping process uncovered putative causally associated variants whose proximal genes were linked with major depression pathophysiology. Four colocalizing genetic variants altered the expression of five genes, highlighting the role of SLC12A5 in neuronal chlorine homeostasis and MYRF in nervous system myelination and oligodendrocyte differentiation. The transcription factor binding analysis revealed the potential role of rs62259947 in modulating P4HTM expression by altering the YY1 binding site, altogether regulating hypoxia response. Overall, our pipeline could prioritize putative causal genetic variants in major depression. More importantly, it can be applied when only index genetic variants are available. Finally, the presented approach enabled the proposal of mechanistic hypotheses of these genetic variants and their role in disease pathogenesis.
Identification of Male-Specific Molecular Markers by Recombination of RhoGEF10 Gene in Spotted Knifejaw (Oplegnathus punctatus)
Authors: Wu Yanduo , Xiao Yongshuang , Xiao Zhizhong , Ma Yuting , Zhao Haixia , Li Jun
Published: July 2022

The spotted knifejaw (Oplegnathus punctatus) is a marine economic fish with high ecological value, food value, and fishing value, and its growth has obvious sex dimorphism. The rapid identification of its sex is beneficial to the development of sex determination and breeding. In this study, the method of comparative genomics and PCR amplification was used to further establish a rapid detection method for the recombinant RhoGEF10 gene in O. punctatus, which can quickly, accurately, and efficiently identify the sex of the O. punctatus to be tested. The homologous comparison results of male and female individuals showed that the DNA fragment length of the RhoGEF10 gene on the X1 chromosome was 326 bp, and the DNA fragment length on the Y chromosome was 879 bp. Therefore, it can be concluded that there is an insert fragment of 553 bp on the Y chromosome. PCR amplification results showed that the two DNA fragments of 879 bp and 326 bp were amplified in the Y chromosome and X1 chromosome of the male O. punctatus (X1X2Y), respectively, and the 879 bp fragment was a unique marker fragment of the recombinant RhoGEF10 gene; The female O. punctatus (X1X1X2X2) only a single DNA fragment of 326 bp was amplified. At the same time, the inserted fragment of the male individual resulted in partial inactivation of the RhoGEF10 protein, which in turn resulted in a slowing of peripheral nerve conduction velocity and thinning of the myelin sheath in male O. punctatus. The method shortens the time for accurate identification of the O. punctatus RhoGEF10 gene recombination and improves the detection efficiency. It is of great significance and application value in the research of nerve conduction and myelin development, male and female sex identification, the preparation of high male seedlings, and family selection based on the RhoGEF10 gene in the O. punctatus.
HSPA8 Single-Nucleotide Polymorphism Is Associated with Serum HSC70 Concentration and Carotid Artery Atherosclerosis in Nonalcoholic Fatty Liver Disease
Authors: Zhao Wenli , Mori Hitoe , Tomiga Yuki , Tanaka Kenichi , Perveen Rasheda , Mine Keiichiro , Inadomi Chika , Yoshioka Wataru , Kubotsu Yoshihito , Isoda Hiroshi , Kuwashiro Takuya , Oeda Satoshi , Akiyama Takumi , Zhao Ye , Ozaki Iwata , Nagafuchi Seiho , Kawaguchi Atsushi , Aishima Shinichi , Anzai Keizo , Takahashi Hirokazu
Published: July 2022

There is an association between nonalcoholic fatty liver disease (NAFLD) and atherosclerosis, but the genetic risk of atherosclerosis in NAFLD remains unclear. Here, a single-nucleotide polymorphism (SNP) of the heat shock 70 kDa protein 8 (HSPA8) gene was analyzed in 123 NAFLD patients who had been diagnosed using a liver biopsy, and the NAFLD phenotype including the maximum intima–media thickness (Max-IMT) of the carotid artery was investigated. Patients with the minor allele (A/G or G/G) of rs2236659 showed a lower serum heat shock cognate 71 kDa protein concentration than those with the major A/A allele. Compared with the patients with the major allele, those with the minor allele showed a higher prevalence of hypertension and higher Max-IMT in men. No significant associations between the HSPA8 genotype and hepatic pathological findings were identified. In decision-tree analysis, age, sex, liver fibrosis, and HSPA8 genotype were individually associated with severe carotid artery atherosclerosis (Max-IMT ≥ 1.5 mm). Noncirrhotic men aged ≥ 65 years were most significantly affected by the minor allele of HSPA8. To predict the risk of atherosclerosis and cardiovascular disease, HSPA8 SNP genotyping might be useful, particularly for older male NAFLD patients.
An Intron c.103-3T>C Variant of the AMELX Gene Causes Combined Hypomineralized and Hypoplastic Type of Amelogenesis Imperfecta: Case Series and Review of the Literature
Authors: Leban Tina , Trebušak Podkrajšek Katarina , Kovač Jernej , Fidler Aleš , Pavlič Alenka
Published: July 2022

Amelogenesis imperfecta (AI) is a heterogeneous group of genetic disorders of dental enamel. X-linked AI results from disease-causing variants in the AMELX gene. In this paper, we characterise the genetic aetiology and enamel histology of female AI patients from two unrelated families with similar clinical and radiographic findings. All three probands were carefully selected from 40 patients with AI. In probands from both families, scanning electron microscopy confirmed hypoplastic and hypomineralised enamel. A neonatal line separated prenatally and postnatally formed enamel of distinctly different mineralisation qualities. In both families, whole exome analysis revealed the intron variant NM_182680.1: c.103-3T>C, located three nucleotides before exon 4 of the AMELX gene. In family I, an additional variant, c.2363G>A, was found in exon 5 of the FAM83H gene. This report illustrates a variant in the AMELX gene that was not previously reported to be causative for AI as well as an additional variant in the FAM83H gene with probably limited clinical significance.
Calcite Deformation Twins: From Crystal Plasticity to Applications in Geosciences
Authors: Lacombe Olivier
Published: July 2022

E-twinning is the dominant mechanism of plastic deformation of calcite at low temperature (<300 °C), and in most limestones, e-twins are, at the crystal scale, the dominant microstructures [...]
Professional Identity Scale for Male Nursing Students Using the Rasch Model and Latent Regression on Gender and Background Variables
Authors: Li Renhau , Lou Jiunnhorng
Published: July 2022

This study developed a professional identity (PI) scale and compared the scores of male and female nursing students. Few studies have focused on male nursing students to understand their PI vis-à-vis related background variables relative to female ones. We recruited 384 male nursing students in Taiwan to construct the PI scale based on the Rasch model with 12 items and 3 factors, namely cognitive, emotional, and behavioural identity. The PI scale showed a good model fit in confirmatory factor analysis, with factor loadings ranging from 0.56 to 0.73. Cronbach’s alpha coefficients ranged from 0.72 to 0.79 for the three subscales and 0.88 for the entire scale. The results of the multiple latent regression analyses showed that male nursing students had higher PI in the total scale and its three factors than did female ones. Having mothers with medical or nursing-related jobs may help promote the cognitive PI of male nursing students. Experiences of caring for family members can help promote PI among female nursing students but not among male ones. Future research should focus on decreasing loss in behavioural PI for both genders after graduation and on reinforcing the association between behavioural PI and interest in nursing among male nursing students.
A Retrospective Cohort Study on the Clinical Characteristics of Patients with Surgical Blunt Bowel and/or Mesenteric Injuries among Motorcyclists and Car Occupants
Authors: Hsieh Ting-Min , Chuang Po-Chun , Liu Chun-Ting , Wu Bei-Yu , Hsieh Ching-Hua
Published: July 2022

(1) Background: Surgical blunt bowel and/or mesenteric injuries (BBMIs) are rare but challenging for trauma surgeons. Surgical BBMI is associated with specific injury mechanisms, such as direct compression by the handlebar in motorcycle accidents or rapid acceleration and deceleration of the impact forces associated with seatbelt injuries in motor vehicle collisions. However, the discussions on the implications of BBMI and the mechanisms of road traffic accidents remain scarce. This retrospective study assessed the clinical and injury characteristics of surgically proven BBMI among motorcyclists and car occupants based on trauma-registered data obtained from a level I trauma center in Taiwan. (2) Methods: Medical data of 72 motorcyclists and 38 car occupants who had surgical BBMI between January 2009 and December 2020 were reviewed. Patient characteristics, injuries, and outcomes in both groups were compared and analyzed. (3) Results: Motorcyclists with surgical BBMI had a significantly higher Injury Severity Score (median [Q1–Q3], 18 (9–27) vs. 16 (9–18), p = 0.044) and lower Glasgow Coma Scale score (15 (11–15) vs. 15 (15–15), p = 0.034]) than car occupants. Motorcyclists with surgical BBMI had a higher incidence of pelvic fractures (18.1% vs. 2.6%, p = 0.032) and upper limb fractures (23.6% vs. 7.9%, p = 0.042) and a significantly higher rate of chest tube insertion than car occupants (29.2% vs. 10.5%, p = 0.027). However, there were no significant differences in the outcomes of morbidity and mortality between motorcyclists and car occupants with surgical BBMI. (4) Conclusions: This study demonstrated there were no significant differences in outcomes between motorcyclists and car occupants with surgical BBMI. However, motorcyclists with surgical BBMI were injured more severely, along with injuries to the head/neck and extremities, than car occupants.
Stimuli Influencing Engagement, Satisfaction, and Intention to Use Telemedicine Services: An Integrative Model
Authors: Amin Ruhul , Hossain Md. Alamgir , Uddin Md. Minhaj , Jony Mohammad Toriqul Islam , Kim Minho
Published: July 2022

Telemedicine ensures quality, cost-effective, and equally accessible healthcare services for everyone. Nonetheless, a poor usage rate could curb its progression in developing cultures like Bangladesh. Therefore, this research examines how external stimuli promote the continuous usage intentions of synchronous telemedicine services through engagement and satisfaction by deploying the stimulus-organism-response framework. A final sample of 312 telemedicine users was analyzed using the structural equation modeling in AMOS. The average age of the participants was 26.28 (std. deviation 5.53), and their average use of telemedicine was 2.39 times (std. deviation 1.31) over the last six months. This study empirically endorsed that the stimuli, including performance expectancy, information quality, and contamination avoidance, as well as organismic factors such as engagement and satisfaction, directly impacted the continuance desires for telemedicine use. In addition, the analyses validated the mediation roles of engagement and satisfaction. Furthermore, performance and effort expectancies influenced engagement, which affected satisfaction along with performance expectancy, functionality, and information quality. Accordingly, telemedicine facilitators should integrate these critical attributes into the system to sustain engagement, satisfaction, and usage intentions. This study has pioneered the effects of performance and effort expectancies on continuous usage intentions facilitated by engagement and satisfaction in the telemedicine landscape.