bluuebunny/crossref_metadata_2025 huggingface dataset:
Efeitos da facilitação neuromuscular propioceptiva sobre a sarcopenia de idosos: revisão integrativa
Authors: Souza Eduardo Maciel , Silva Geraedson Aristides da , Alves Ahyas Sydcley Santos
Published: August 2022

A facilitação neuromuscular proprioceptiva foi desenvolvida pelo o Dr. Herman Kabat e Margareth em 1940. É mais que uma simples técnica, é uma filosofia de tratamento baseada no conceito de que todo ser humano, inclusive os portadores de deficiência, tem um potencial não explorado. O estudo trata-se de uma revisão integrativa que objetivou verificar os efeitos da facilitação neuromuscular proprioceptiva sobre a sarcopenia em idosos, através de publicações selecionadas das bases de dados: LILACS, PubMed, e Scielo nas línguas portuguesa e inglesa. Após a aplicação dos critérios foram incluídos para a revisão 5 artigos, aqueles que abordassem os conceitos sobre sarcopenia em idosos e que explicasse a FNP e o efeito da técnica sobre a sarcopenia de idosos. Através dessa revisão constatou-se nos estudos revisados que o FNP tem efeito significativamente positivos na manutenção da fibra muscular de idosos com sarcopenia melhorando a força e trofismo se mostrando eficaz no tratamento da sarcopenia no idoso. Desta forma a FNP pode ser uma intervenção fisioterapêutica tanto preventiva como recuperadora da qualidade de vida do idoso, minimizando assim, os efeitos da sarcopenia e os impactos trazidos sobre a vida de idosos, entretanto, sugere-se novas pesquisas clínicas com um número maior de pessoas idosas associadas a aplicação da técnica e de outras técnicas visando esclarecer ainda mais os efeitos do FNP sobre a saúde e qualidade de vida desta população.
Correlations among nutrient contents in soil, leaf, and fruit tissues and yield of Olea europaea L. cv. Arbequina in the South of Brazil
Authors: Figueiredo Maria Carolina dos Santos , Rosseto Vanessa , Barão Carine Freitas , Nunes Rosângela Silva Gonçalves , Duarte Iolanda da Luz , Weber Mirla Andrade , Vieira Frederico Costa Beber
Published: August 2022

The objective of the work was to relate the nutritional status of olive orchards from the central region of the Rio Grande do Sul with fruit yield and identify unadjusted points in the fertilization recommendation system to be improved. Soil, leaf, and fruit samples were taken from eight orchards, between 2018 and 2020, and were evaluated for the contents of N, P, K, B, pH, TOC, Al+3, and soil texture. The 20-40 cm layer in soil showed acidity and Al+3 content at potentially restrictive levels. The available P and B contents in soil were considered low. In leaf tissue, K and N presented high contents. The correlations revealed that fruit yield was limited by high N contents on leaves, especially in winter. B content in fruits diminished in higher soil pH, suggesting that the B threshold value in soil for olives in the south of Brazil should be pH-dependent.
Modelo de atenção dos centros de parto normal peri-hospitalares: protocolo de revisão de escopo
Authors: Aguiar Cláudia de Azevedo , Lopes Gisele Almeida , Bussadori Jamile Claro de Castro , Leister Nathalie , Riesco Maria Luiza Gonzalez , Girão Fernanda Berchelli
Published: August 2022

Os centros de parto normal peri-hospitalares (CPNp) são instituições de saúde que oferecem atendimento pré, intra e pós-parto a mulheres e bebês saudáveis, destacando-se por sua filosofia e prática amparadas no modelo humanizado e biopsicossocial de cuidado. Apesar de apresentarem excelentes resultados e indicadores, estas instituições encontram dificuldades para sua manutenção e expansão no Brasil. Este artigo tem como objetivo descrever o protocolo da revisão de escopo que mapeará as características do modelo de atenção dos CPNp brasileiros. O protocolo foi registrado na plataforma Open Science Framework (OSF) e elaborado segundo recomendações do JBI Manual for Evidence Synthesis e PRISMA-ScR. As etapas da revisão são: a definição dos descritores e dos critérios de elegibilidade, a busca dos estudos nas bases de dados e repositórios, a seleção e a extração dos dados dos estudos e, finalmente, como os resultados serão apresentados. Com esta revisão, espera-se mapear o conhecimento científico existente sobre como os CPNp estão estruturados e organizados, como são acessados pela população, quais são os desfechos da assistência oferecida a mulheres e bebês e como usuários e profissionais vivenciam esse modelo de atenção. Além disso, este estudo tem o potencial de contribuir para o debate que se faz urgente frente à construção de estratégias voltadas a melhorias dos indicadores de saúde materno-infantil do país.
Estruturação de um produto educacional em saúde sobre o Método Canguru: relato de experiência com a utilização do Método CTM3
Authors: Medeiros Mirelle Alessandra Silva de , Santos Almira Alves dos , Bandini Heloísa Helena Motta , Santos Jaqueline Maria Silva dos , Silva Vitória Rejane de Lira Ferreira
Published: August 2022

Este artigo aborda a estruturação de um produto educacional para utilização no ensino em saúde sobre o Método Canguru. Trata-se de um estudo descritivo do tipo relato de experiência, com abordagem qualitativa, com o objetivo de apresentar a construção de um vídeo educativo. A estruturação baseou-se no Método CTM3, composto por elementos didaticamente agrupados em 03 etapas: Concepção do produto (C); Referencial teórico (T); e Referencial metodológico fundamentado em três teorias (M3): Análise Transacional, que trabalha com os mecanismos do arcabouço de personalidade que moldam os estados de EGO (Pai, Adulto e Criança); Multisensorialidade, que envolve a percepção dos cinco sentidos (audição, visão, olfato, gustativo e tato/sinestésico); e Programação Neurolinguistica (PNL), que aborda aspectos subliminares da comunicação com a utilização de ferramentas, destacando-se aqui a âncora. Os produtos educacionais são importantes instrumentos de ensino. Os vídeos educativos têm sido utilizados em diversas experiências pedagógicas, demonstrando-se a sua relevância para o processo ensino-aprendizagem. A necessidade de correta estruturação é essencial para a utilização adequada de um recurso educacional e de acordo com os objetivos de aprendizagem. Neste caminho, o Método CTM3 direciona para o saber e como fazer os diversos tipos de produtos educacionais. Preocupa-se com o impacto e abrangência dos recursos educacionais, trabalhando a comunicação e reforçando as potencialidades para a aquisição de conhecimento e melhorar a experiência da aprendizagem. Mostrou-se eficaz no desenvolvimento da comunicação para a educação profissional em saúde, com uma estruturação bem planejada e coerente com a qualidade técnico-científica.
Influence of preoperative serum albumin levels on postoperative complications in cardiac surgery
Authors: Costa Mario Augusto Cray da , Botta Vanessa Carolina , Schafranski Marcelo Derbli , Reis Elise Souza dos Santos , Küchler Stella , Souza Ana Carolina Mello Fontoura de
Published: August 2022

Objective: Verify whether lower preoperative serum albumin levels in cardiac surgery patients increase the incidence of acute kidney injury (AKI), pneumonia, postoperative stay in hospital and mortality. Methodology: A hundred and eighty-five medical records of patients that underwent cardiac surgery between January 2014 and December 2017 were surveyed. The albumin levels were compared between the group that presented at least one of the complications listed above and the group where that complication was not observed. The KDIGO criterion was used to define AKI. Pneumonia diagnosis was established based on clinical, radiologic and laboratory results. The postoperative time in hospital was considered increased when the patient had to stay for a period longer than 8 days. The mortality rate was defined by deaths that occurred up to 30 days after the surgery. Results: Albumin levels ≤3.87 mg/dL were associated to an increase in the risk to develop AKI (p= 0,0072). Albumin values ≤3.91 also increased the chance to have pneumonia (p= 0.0001). However, albumin was not statistically significant to predict mortality and increase in the postoperative stay in hospital. Conclusion: Serum albumin levels that were lower than or equal to 3.91 mg/dL and 3.87 mg/dL increased, respectively, the chances of developing both pneumonia and acute kidney injury after cardiac surgery. No significant results were obtained regarding albumin levels influence on postoperative stay in hospital or mortality.
Giant cell tumor of bone: Epidemiological profile (fifth version) – A study protocol
Authors: Becker Ricardo Gehrke , Pereira Bruno Antunes , Galia Carlos Roberto , Cadore Otavio Pereira , Führ Bruno , Pestilho Julie Francine Cerutti Santos , Macedo Carlos Alberto Souza , Rabusxke Willian Bernardo Spechet , Rodrigues Eduardo da Silva , Guedes Alex
Published: August 2022

The giant cell tumor of bone (GCTB) represents approximately 5% of primary bone tumors, and more than half of cases affect individuals between 20 and 50 years of age. Despite being a benign neoplasm, 3 to 5% of cases may show pulmonary metastases, besides it has high potential for bone destruction at the main site of the lesion, often in the epiphyses of long bones, which may lead to serious functional problems in affected patients. Multidisciplinary treatment of GCTB has been a cause for discussion in the last decade due to the discovery of drugs such as denosumab that prevent tumor progression and may be useful in cases of patients with local recurrences and unresectable tumors. The therapeutic basis of GCTB remains the isolated surgical treatment by intralesional (with adjuvants), marginal or wide resection. Intralesional treatment presents higher rates of recurrence, however it preserves the adjacent joint function and much of the individual bone stock. Resection with wide margins presents lower recurrence rates but has shown worse functional results and frequent complications in bone reconstructions. Current knowledge of GCTB surgical outcomes has been based on major world series - there is a lack of Brazilian epidemiological and surgical data on this specific type of neoplasm, except for a few series of cases. The primary objective of this paper is to identify the epidemiological profile of GCTB; the secondary objectives are to describe the surgical aspects in the main diagnostic centers and treatment of bone tumors, to analyze the clinical and surgical aspects of patients with GCTB of wrist and hand, and to analyze the clinical and surgical aspects of patients with knee GCTB. These findings may help in the maintenance or change of national guidelines for the treatment of GCTB. This study protocol was submitted to ethical assessment under the CAAE (Ethical Appreciation Presentation Certificate) registry No. 94280918.0.0000.5327 (5th version), evaluated through the substantiated opinion No. 4.770.705, and approved by the Institutional Research Ethics Committee on June 12, 2022.
Expressões faladas por vacinados: um momento de prevenção
Authors: Galvão Amanda de Oliveira , Oliveira Elias José , Pereira Janaina Aguero , Martins Marilene Ferreira
Published: August 2022

O objetivo desta pesquisa foi compreender e relatar a percepção dos adultos trabalhadores no momento da vacinação. Os 33 adultos selecionados para o questionário, com abordagem quantitativa e qualitativa, que analisou as falas e as frases durante o processo de vacinação extra muro. A entrevista compreendeu homens e mulheres acima de 18 anos. Os resultados demonstraram que o efeito manada impactando na imunização dos adultos em vacinação extra muro com falas remetendo ao processo de dor e angustia associado a sofrimento coletivo. O maior destaque das expressões relatadas foram “medo” e “agonia”. É interessante notar que todas as expressões carregam intrinsecamente uma mistura de dor, medo, imposição e pavor, associado ao processo de vacinação e memórias afetivas/traumáticas do passado da população. O profissional da enfermagem nessa perspectiva tem um papel essencial de educador e fiscalizador da prestação de serviços a saúde na sala de vacina. Como é um profissional que atua diretamente com a população tem a responsabilidade de desmistificar dúvidas quanto à eficácia e os possíveis eventos adversos decorrentes da vacinação, de forma a fortalecer o PNI e consequentemente promover maior adesão ao programa.
Identificação e análise de intervenção em um profissional com 30 anos com TDAH
Authors: Mello Bruna Leticia Cagalli de , Sales Roberto Lopes , Braga Francisco Carneiro , De Oliveira Hellyegenes , Nobre Juliana , Pereira Ailton Leal , Souza Alcione Santos de , Santos Alex Monteiro dos , Duque Rita de Cássia Soares
Published: August 2022

O estudo aborda sobre a questão de um profissional com idade de 30 anos com suspeita da TDAH. Esse é um conceito que chama a atenção de pesquisadores no contexto da educação inclusiva. Percebe-se que no Brasil um paradoxo é existente entre o que as leis pregam e o que é de fato realizado na prática. As empresas ainda não estão preparadas para lidar com profissionais que tenham essas características. Assim, esse artigo teve como objetivo abordar sobre os desafios da inclusão profissional de uma pessoa com TDAH. Dessa forma, tratou-se de um estudo bibliográfico, qualitativo, exploratório e explicativo, que integram o arcabouço metodológico do estudo. A prospecção de dados foi realizada na base de dados Biblioteca Digital de Teses e Dissertações, artigos científicos e demais meios de comunicação acadêmicos. O estudo demonstrou como resultados: a) a existência de características de um profissional que mostra algumas características de TDAH. E no que se refere as conclusões, o estudo pode concluir que a temática do TDAH nas organizações precisa ser mais aprofundada no que tange ao seu debate e sugestiona a participação familiar e dos demais profissionais que atuam satélites a esse paciente, como uma forma de tornar mais profícua a inclusão profissional em todos os processos decisórios da organização. O desafio maior de uma empresa é um viés inclusivo é o da garantia de acesso e permanência desses profissionais em todos os processos existentes nas empresas, com vistas ao alcance de sua maior eficiência nas suas diversas atividades profissionais, além de ter e exercer todos os seus direitos de cidadão.
Simulação da trajetória e magnitude de meteoroides de pequena massa
Authors: Bruzigues Christian Andrade , Soltau Samuel Bueno
Published: August 2022

Através de simulações computacionais estuda-se o comportamento de meteoroides ao adentrar a atmosfera terrestre. Dados os parâmetros massa e velocidade iniciais aplica-se o método numérico de Integração de Euler Modificado para resolver as equações que descrevem a o comportamento de meteoroides em queda. Os resultados numéricos obtidos através de valores arbitrários inseridos na simulação mostraram boa concordância quando comparados com os observáveis selecionados da base de dados da International Astronomical Union (IAU), porém evidenciam que aperfeiçoamentos na precisão podem ser realizados em versões futuras do algoritmo.
Dampak Konflik Perang Ganda dan Self Efficacy terhadap Kinerja Karyawan Perbankan di Semarang
Authors: Haziroh Awanis Lianti , Pramadanti Amanda Dyla , Putri RA Aminah Rizki
Published: August 2022

Kehidupan modern saat ini memiliki banyak masalah yang dihadapi para pekerja dimana konflik pekerjaan dan keluarga merupakan salah satu faktor yang memiliki pengaruh yang cukup besar. Konflik antara pekerjaan dan keluarga dapat berdampak pada kinerja karyawan. Untuk meningkatkan kinerja sebuah perusahaan dibutuhkan kinerja karyawan yang tinggi. Perusahaan untuk memiliki sumber daya manusia yang memiliki kinerja yang tinggi maka diperlukan kualitas sumber daya manusia yang tinggi dan untuk mendapatkan kualitas sumber daya manusia yang baik diperlukan adanya self efficacy yang tinggi. Penelitian bertujuan untuk menganalisis pengaruh konflik peran ganda terhadap kinerja karyawati di perbankan yang dimoderasi oleh self efficacy. Jenis penelitian adalah penelitian kuantitatif. Populasi penelitian adalah seluruh karyawati yang bekerja di perbankan. Teknik pengumpulan data menggunakan kuesioner dengan mengam-bil 100 responden sesuai dengan kriteria yakni bekerja minimal selama 1 tahun dan sudah menikah. Alat analisis yang digunakan pada penelitian ini menggunakan Moderated Regression Analysis (MRA). Berdasarkan hasil penelitian diperoleh hasil pada model pertama menunjukkan bahwa variabel konflik peran ganda berpengaruh secara negatif dan signifikan terhadap kinerja karyawati. Model kedua menunjukkan bahwa self efficacy berpengaruh secara positif dan signifikan terhadap kinerja karyawati. Model ketiga menunjukkan bahwa self efficacy bukan merupakan variabel pemoderasi antara konflik peran ganda dengan kinerja karyawati.
AN EFFECT OF HALAL’S DIMENSIONS ON SUBSCRIPTION TO HALAL RESTAURANTS IN JAKARTA
Authors: Budiman, SE., M.Buss. Andika Nuraga , Suyana Hilma , Nurhayati Ety
Published: August 2022

This research objective is to investigate the influence of the Halal dimension on subscribing Halal restaurants. This research objective is to investigate the influence of the Halal dimension on subscribing to Halal restaurants. This research method was using a questionnaire spread to 169 respondents in the Central Jakarta area in Cempaka Putih. The purposive sampling especially for Muslims was an absolute condition to become this research sample. The result shows that interesting result that Muslims in Jakarta, which are we expecting these results are representing the majority of the population, evidently our respondents are not paying attention to the Halal process before they visit a restaurant, which is one of the process processes is animal slaughtering process. An explanation of the relationship between Halal certification and subscribing. Halal restaurants have a significant relationship. apart from the regression results, the results of the T test have explained that the existence of Halal certification is very important. Also, Halal accreditation and their awareness need to be improved, there isnot significant toward subscribing noto Halal restaurants. The main part is religiosity which is a very strong religious status for Muslims; this is one of the reasons why Muslims always subscribe to restaurants because they believe wherever there is a religious status that is still firmly held.
PENGARUH PENERAPAN GOOD CORPORATE GOVERNANCE (GCG) , KESADARAN ANTI-FRAUD, DAN INTEGRITAS KARYAWAN TERHADAP PENCEGAHAN KECURANGAN (FRAUD)
Authors: Budiantoro, SE. M.Ak. Ak. CA. CSP Harry , Aprillivia Nanda Dwi , Lapae Kanaya
Published: August 2022

Penelitian ini bertujuan untuk menguji pengaruh good corporate governance (GCG), fraud awareness dan employee integrity terhadap pencegahan fraud. Data pertama berupa kuesioner yang digunakan dalam penelitian ini. Dalam penelitian ini, responden bertanggung jawab atas customer support dan financial services atau melakukan kontak langsung dengan klien dan di bidang informasi nasabah dan situasi keuangan secara langsung di Bank BTN. Berdasarkan metode sampling jenuh, jumlah responden dalam survei ini adalah 50. Hasil penelitian ini menunjukkan bahwa penerapan tata kelola perusahaan yang baik, kesadaran anti-fraud dan integritas karyawan berpengaruh signifikan terhadap pencegahan fraud.
PENGARUH KUALITAS YANG DIPERSEPSIKAN TERHADAP KEPUTUASAN PEMBELIAN MELALUI KEPERCAYAAN MEREK SEBAGAI VARIABEL MEDIASI PADA PENGGUNA PRODUK PERAWATAN KULIT CITRA
Authors: Lutfiyah Lutfiyah , Annisa Intan Tri
Published: August 2022

Penelitian ini bertujuan untuk mengetahui Pengaruh Kualitas yang Dipersepsikan terhadap Keputusan Pembelian melalui Kepercayaan Merek sebagai Variabel Intervening serta tinjauan dari sudut Pandang Islam. Populasi dalam penelitian pengguna Hand and body Lotion Citra di Cianjur. Jumlah responden pada penelitian ini sebanyak 91orang. Data dikumpulkan dengan menggunakan metode survey dengan instrument kuesioner. Metode analisis data yang digunakan yaitu analisis Partial Least Square Structural Equation Modelling. Hasil penelitian menunjukkan bahwa: (1) kualitas yang dipersepsikan berpengaruh positif dan signifikan terhadap keputusan pemeblian (p-value: 0.001) (2) kualitas yang dipersepsikan berpengaruh positif dan signifikan terhadap kepercayaan merek (p-value: 0.001) (3) kepercayaan merek berpengaruh positif dan signifikan terhadap keputusan pembelian (p-value: 0.001) (4) kepercayaan merek dapat memediasi pengaruh kualitas yang dipersepsikan terhadap keputusan pembelian (p-value: 0.00Kata Kunci  : Kualitas yang Dipersepsikan, Keputusan Pembelian, Kepercayaan Merek
PENERAPAN SISTEM INFORMASI MANAJEMEN KEUANGAN PADA PEMERINTAH DESA DI KECAMATAN BANGSALSARI KABUPATEN JEMBER
Authors: Pratiwi Ana
Published: August 2022

Abstract               The Law on Villages, namely Law number 6 of 2014, mandates the village apparatus, which we usually refer to as the village government, to prepare the village to be more independent. Independent here is, the village government can process the natural resources owned by the village and manage the government well. These resources include the wealth belonging to the village, which can be in the form of natural wealth, famous figures or cultural sites in the village, and managing village finances. In Ministerial Regulation Number 113 of 2014, it is clear that village financial management must be in accordance with what has been written in this Ministerial Regulation. In this regulation, it is explained that village financial management is the rights and obligations of the village, which in this case is represented by the village apparatus, measured by an amount of money or anything that can be in the form of money and goods related to the implementation of village rights and obligations.This research is a quantitative research with the population of all village officials in the Bangsalsari district. Determination of the sample using a saturated sample where entire population is used as a sample. This study examines the effect of system quality and information quality will affect user satisfaction. This research results that the quality of the system has no significant effect on user satisfaction, while the quality of information has a significant effect on user satisfaction. This can happen because the respondents think that the quality of the system in government agencies tends to be less good, while the information system itself is considered to be able to increase satisfaction due to the times that support the quality of the information system to be good.Keywords:Quality of system, quality of information, user satisfication
Flerkulturalitet i opplæring av helsefagarbeidere
Authors: Bergsli Oddhild Peggy
Published: August 2022

This study will focus on multicultural competence as a topic in vocational training of health care workers in upper secondary school in Norway. The research question is: How do vocational teachers practice multiculturalism in health care worker vocational education? According to OECD, multiculturalism is one of the core competencies of the 21st century. The need of healthcare professionals with expertise in multicultural sensitivity and health-communication will increase in the years ahead because this competence is essential for a good and caring professional health care. The theoretical perspective is related to a theoretical conceptual understanding of multicultural competence and to vocational didactic perspectives on health care worker vocational training.

The data collection is based on semi-structured interviews with vocational teachers. The key findings show that multicultural issues are rarely highlighted in education and that multicultural topics are rarely linked to vocationally relevant professional tasks. Topics as health-communication and diet were poorly elucidated from a multicultural perspective. There will be a need to implement various occupationally relevant multicultural issues in health worker vocational training to prepared for the multicultural professional life.
Timely delivery of cardiac mmRNAs in microfluidics enhances cardiogenic programming of human pluripotent stem cells
Authors: Contato Anna , Gagliano Onelia , Magnussen Michael , Giomo Monica , Elvassore Nicola
Published: August 2022

In the last two decades lab-on-chip models, specifically heart-on-chip, have been developed as promising technologies for recapitulating physiological environments suitable for studies of drug and environmental effects on either human physiological or patho-physiological conditions. Most human heart-on-chip systems are based on integration and adaptation of terminally differentiated cells within microfluidic context. This process requires prolonged procedures, multiple steps, and is associated with an intrinsic variability of cardiac differentiation. In this view, we developed a method for cardiac differentiation-on-a-chip based on combining the stage-specific regulation of Wnt/β-catenin signaling with the forced expression of transcription factors (TFs) that timely recapitulate hallmarks of the cardiac development. We performed the overall cardiac differentiation from human pluripotent stem cells (hPSCs) to cardiomyocytes (CMs) within a microfluidic environment. Sequential forced expression of cardiac TFs was achieved by a sequential mmRNAs delivery of first MESP1, GATA4 followed by GATA4, NKX2.5, MEF2C, TBX3, and TBX5. We showed that this optimized protocol led to a robust and reproducible approach to obtain a cost-effective hiPSC-derived heart-on-chip. The results showed higher distribution of cTNT positive CMs along the channel and a higher expression of functional cardiac markers (TNNT2 and MYH7). The combination of stage-specific regulation of Wnt/β-catenin signaling with mmRNAs encoding cardiac transcription factors will be suitable to obtain heart-on-chip model in a cost-effective manner, enabling to perform combinatorial, multiparametric, parallelized and high-throughput experiments on functional cardiomyocytes.
Quantum-Inspired Moth-Flame Optimizer With Enhanced Local Search Strategy for Cluster Analysis
Authors: Cui Xinrong , Luo Qifang , Zhou Yongquan , Deng Wu , Yin Shihong
Published: August 2022

Clustering is an unsupervised learning technique widely used in the field of data mining and analysis. Clustering encompasses many specific methods, among which the K-means algorithm maintains the predominance of popularity with respect to its simplicity and efficiency. However, its efficiency is significantly influenced by the initial solution and it is susceptible to being stuck in a local optimum. To eliminate these deficiencies of K-means, this paper proposes a quantum-inspired moth-flame optimizer with an enhanced local search strategy (QLSMFO). Firstly, quantum double-chain encoding and quantum revolving gates are introduced in the initial phase of the algorithm, which can enrich the population diversity and efficiently improve the exploration ability. Second, an improved local search strategy on the basis of the Shuffled Frog Leaping Algorithm (SFLA) is implemented to boost the exploitation capability of the standard MFO. Finally, the poor solutions are updated using Levy flight to obtain a faster convergence rate. Ten well-known UCI benchmark test datasets dedicated to clustering are selected for testing the efficiency of QLSMFO algorithms and compared with the K-means and ten currently popular swarm intelligence algorithms. Meanwhile, the Wilcoxon rank-sum test and Friedman test are utilized to evaluate the effect of QLSMFO. The simulation experimental results demonstrate that QLSMFO significantly outperforms other algorithms with respect to precision, convergence speed, and stability.
High-throughput bioengineering of homogenous and functional human-induced pluripotent stem cells-derived liver organoids via micropatterning technique
Authors: Xu Xiaodong , Jiang Shanqing , Gu Longjun , Li Bin , Xu Fang , Li Changyong , Chen Pu
Published: August 2022

Human pluripotent stem cell-derived liver organoids are emerging as more human-relevant in vitro models for studying liver diseases and hepatotoxicity than traditional hepatocyte cultures and animal models. The generation of liver organoids is based on the Matrigel dome method. However, the organoids constructed by this method display significant heterogeneity in their morphology, size, and maturity. Additionally, the formed organoid is randomly encapsulated in the Matrigel dome, which is not convenient for in situ staining and imaging. Here, we demonstrate an approach to generate a novel type of liver organoids via micropatterning technique. This approach enables the reproducible and high-throughput formation of bioengineered fetal liver organoids with uniform morphology and deterministic size and location in a multiwell plate. The liver organoids constructed by this technique closely recapitulate some critical features of human liver development at the fetal stage, including fetal liver-specific gene and protein expression, glycogen storage, lipid accumulation, and protein secretion. Additionally, the organoids allow whole-mount in-situ staining and imaging. Overall, this new type of liver organoids is compatible with the pharmaceutical industry’s widely-used preclinical drug discovery tools and will facilitate liver drug screening and hepatotoxic assessment.
Bioproduction of propionic acid using levulinic acid by engineered Pseudomonas putida
Authors: Tiwari Rameshwar , Sathesh-Prabu Chandran , Lee Sung Kuk
Published: August 2022

The present study elaborates on the propionic acid (PA) production by the well-known microbial cell factory Pseudomonas putida EM42 and its capacity to utilize biomass-derived levulinic acid (LA). Primarily, the P. putida EM42 strain was engineered to produce PA by deleting the methylcitrate synthase (PrpC) and propionyl-CoA synthase (PrpE) genes. Subsequently, a LA-inducible expression system was employed to express yciA (encoding thioesterase) from Haemophilus influenzae and ygfH (encoding propionyl-CoA: succinate CoA transferase) from Escherichia coli to improve the PA production by up to 10-fold under flask scale cultivation. The engineered P. putida EM42:ΔCE:yciA:ygfH was used to optimize the bioprocess to further improve the PA production titer. Moreover, the fed-batch fermentation performed under optimized conditions in a 5 L bioreactor resulted in the titer, productivity, and molar yield for PA production of 26.8 g/L, 0.3 g/L/h, and 83%, respectively. This study, thus, successfully explored the LA catabolic pathway of P. putida as an alternative route for the sustainable and industrial production of PA from LA.
Nucleic acid amplification strategies for volume-amplified magnetic nanoparticle detection assay
Authors: Huang Zhongchao , Li Jing , Zhong Hongwen , Tian Bo
Published: August 2022

Magnetic nanoparticles (MNPs) can be quantified based on their magnetic relaxation properties by volumetric magnetic biosensing strategies, for example, alternating current susceptometry. Volume-amplified magnetic nanoparticle detection assays (VAMNDAs) employ analyte-initiated nucleic acid amplification (NAA) reactions to increase the hydrodynamic size of MNP labels for magnetic sensing, achieving attomolar to picomolar detection limits. VAMNDAs offer rapid and user-friendly analysis of nucleic acid targets but present inherence defects determined by the chosen amplification reactions and sensing principles. In this mini-review, we summarize more than 30 VAMNDA publications and classify their detection models for NAA-induced MNP size increases, highlighting the performances of different linear, cascade, and exponential NAA strategies. For some NAA strategies that have not yet been reported in VAMNDA, we predicted their performances based on the reaction kinetics and feasible detection models. Finally, challenges and perspectives are given, which may hopefully inspire and guide future VAMNDA studies.
Cyclic RGD functionalized PLGA nanoparticles loaded with noncovalent complex of indocyanine green with urokinase for synergistic thrombolysis
Authors: Zhang Sha , Li Jinjie , Ren Jiefeng , Xue Zaiyao , Qi Xinlian , Si Quanjin
Published: August 2022

Thrombotic diseases have the characteristics of long latency period, rapid onset, and high mortality rate, which seriously threaten people’s life and health. The aim of this research is to fabricate a novel indocyanine green complex of urokinase (ICG@uPA) and employ the amphiphilic PEG-PLGA polymer to deliver the complex as an enzyme-phototherapeutic synergistic thrombolysis platform. The noncovalent indocyanine green (ICG) complex of urokinase (ICG@uPA) was prepared via supramolecular self-assembly and then encapsulated into cRGD decorated polymeric nanoparticles (cRGD-ICG-uPA NPs) by double-emulsion solvent evaporation method. Then the nanoparticles (NPs) were characterized in terms of particle size, optical properties, in vitro release, etc. The targeting and thrombolytic effect of the nanoparticles were studied both in vitro and in vivo. ICG@uPA and cRGD-ICG-uPA NPs displayed significantly higher photostability and laser energy conversion efficiency than free ICG. Concomitantly, the NPs exhibited selective binding affinity to the activated platelets and specific accumulation in the mouse mesenteric vessel thrombus. Significant thrombolysis was achieved in vivo by photo-assisted synergistic therapy with reduced dose and systemic bleeding risk of uPA. Our results prove that the functional PLGA nanoparticle loaded with the ICG@uPA offers a novel option for effective and safe thrombolytic treatment.
Highly efficient and automated isolation technology for extracellular vesicles microRNA
Authors: Di Kaili , Fan Boyue , Gu Xinrui , Huang Rongrong , Khan Adeel , Liu Chang , Shen Han , Li Zhiyang
Published: August 2022

MicroRNA (miRNA) in extracellular vesicles (EVs) has great potential to be a promising marker in liquid biopsy. However, the present EV isolation methods, such as ultracentrifugation, have complicated and long-time operation, which impedes research on EV miRNA. The downstream complex miRNA extraction process will also significantly increase the detection cycle and loss. We first established a simple automated technique to efficiently extract target miRNAs in EVs from plasma based on Fe3O4@TiO2 beads with high affinity and capture efficiency. We combined a heat-lysis method for quick and simple EV miRNA extraction and detection. The results indicated that our method has more RNA yield than TRIzol or a commercial kit and could complete EV enrichment and miRNA extraction in 30 min. Through the detection of miRNA-21, healthy people and lung cancer patients were distinguished, which verified the possibility of the application in clinical detection. The automated isolation technology for EV miRNA has good repeatability and high throughput, with great application potential in clinical diagnosis.
Biosynthesis of novel metallic silvers on kraft papers using cephalotaxus harringtonia fruit extract as a sustainable stabilizing agent (KP@AgNP)
Authors: Wei Shaofeng , Liu Xiaoyi , Xie Jiao , Liu Huijuan , Zeng Qibing , wang Guoze , Luo Peng
Published: August 2022

Greenly synthesized silver nanoparticles (AgNPs) on different cellulosic materials show tremendous potential for colorful, biocidal, and reasonably strong products by replacing the traditional chemical-based synthesis protocols. This study reports on a novel in situ synthesis protocol for synthesizing green and sustainable AgNPs over cellulosic kraft paper substrates using a bio-based stabilizing agent (Cephalotaxus harringtonia fruit extract). The protocol could play a significant role in packaging industries. The aqueous extracts of Cephalotaxus harringtonia fruits have been used to synthesize the metallic silver. The deposited AgNPs values were investigated through XRF (X-ray fluorescence) analysis. The number of deposited nanoparticles (NPs) was 268 ± 7, 805 ± 14, and 1,045 ± 16 PPM, respectively for 0.5, 1.5, and 2.5 mm silver precursors. The developed products were tested with SEM (scanning electron microscopy), SEM-mediated elemental mapping, EDX (energy disruptive X-ray), FTIR (Fourier transform infrared spectroscopy), and XRD (X-Ray diffraction). XRD analysis further confirmed the presence of peaks for elemental AgNP on the deposited papers. Colorimetric values were measured to confirm the colorful appearances of the developed metallic silvers. Mechanical properties were tested in terms of the tensile index and bursting index. Moreover, the statistical analysis of coefficient of variations (R2) and a post-hoc ANOVA test that adopted the Newman-Keul methodology also confirm the significance of developed nanoparticles in the papers. The shielding capacity against UV light was also investigated; all the AgNPs-treated products provided values higher than 40, demonstrating the strong UV resistance capability of the kraft paper material. Overall, the study confirms a successful development of green AgNPs on paper materials.
The role of lncRNAs and XIST in oral cancer
Authors: Liu Huimin , Wang Dongxu , Kan Shaoning , Hao Ming , Chang Lu , Lu Pengxu , Liu Yangyang , Jin Ye , Liu Weiwei
Published: August 2022

Long non-coding RNA (lncRNA) plays a significant role in the pathogenesis of many human malignant tumors, including oral cancer. LncRNA can act as a gene regulator in a variety of cancers. It regulates the growth of malignant cells via many cellular signal pathways such as the PI3K (phosphoinositide 3-kinase)/AKT (α-serine/threonine-protein kinase) pathway. In this review, we have analyzed the role of lncRNAs, such as lncRNA X inactive specific transcript (XIST), in oral cancer, including its effects on the proliferation, apoptosis, invasion, migration, and resistance to chemotherapy of oral cancer. We have also focused on the role of lncRNA XIST as the core of X chromosome inactivation. Here, we provide a brief overview of the role of many kinds of lncRNAs, including XIST, which provides a theoretical basis for the study of the role of XIST in oral cancer. Our review may provide a new direction for the study of the occurrence, development, and prognosis of oral cancer and provide a new target for its treatment.
Spontaneous Activity Predicts Survival of Developing Cortical Neurons
Authors: Warm Davide , Bassetti Davide , Schroer Jonas , Luhmann Heiko J. , Sinning Anne
Published: August 2022

Spontaneous activity plays a crucial role in brain development by coordinating the integration of immature neurons into emerging cortical networks. High levels and complex patterns of spontaneous activity are generally associated with low rates of apoptosis in the cortex. However, whether spontaneous activity patterns directly encode for survival of individual cortical neurons during development remains an open question. Here, we longitudinally investigated spontaneous activity and apoptosis in developing cortical cultures, combining extracellular electrophysiology with calcium imaging. These experiments demonstrated that the early occurrence of calcium transients was strongly linked to neuronal survival. Silent neurons exhibited a higher probability of cell death, whereas high frequency spiking and burst behavior were almost exclusively detected in surviving neurons. In local neuronal clusters, activity of neighboring neurons exerted a pro-survival effect, whereas on the functional level, networks with a high modular topology were associated with lower cell death rates. Using machine learning algorithms, cell fate of individual neurons was predictable through the integration of spontaneous activity features. Our results indicate that high frequency spiking activity constrains apoptosis in single neurons through sustained calcium rises and thereby consolidates networks in which a high modular topology is reached during early development.
Expression profiles of exosomal tRNA-derived fragments and their biological functions in lipomas
Authors: Zhou Yuxi , Tao Daixi , Shao Zifei , Wang Xiang , Xu Jinhao , Li Yiyang , Li Kun
Published: August 2022

There is evidence that exosomes derived from the lipoma tissue (Exo-LT) have a stronger capacity to promote the proliferation and migration of adipose-derived stem cells (ADSCs) than those from the adipose tissue (Exo-AT). But the Exo-LT do not have a significant effect on the adipogenic differentiation of the ADSCs. Recently, certain exosomal tRNA-derived fragments (tRFs) have been shown to play a crucial role in the pathogenesis of certain tumors. Therefore, it is necessary to identify the differently expressed tRFs in Exo-LT to further elucidate their molecular functions in lipomas. High-throughput sequencing was performed to examine the tRFs and mRNAs from the all samples belonging to the Exo-LT and Exo-AT groups. Target prediction and bioinformatics analysis were performed to explore their downstream mRNAs and biological functions. In total, 456 differently expressed tRFs and tiRNAs were identified in the Exo-LT group, 12 of which were up-regulated and 12 were down-regulated, respectively. Notably, tRF-1001 was most obviously down-regulated and tRF-3004a was most obviously up-regulated in the Exo-LT group. Moreover, among the target genes of tRF-1001 and tRF-3004a, both JAG2 and VSIG4 were significantly down-regulated in the Exo-LT group, while WNT5A, COL1A1, and PPARGC1A were highly expressed in both the Exo-LT and Exo-AT groups. The significant down-regulation of JAG2 and VSIG4 in the Exo-LT group could be due to the fact that Exo-LT had a stronger capacity to promote the proliferation and migration of ADSCs compared to the Exo-AT. The high expression of WNT5A, COL1A1, and PPARGC1A in both the Exo-LT and Exo-AT groups could be due to the similar ability of Exo-LT and Exo-AT to promote the adipogenic differentiation of ADSCs.
Elevated levels of the methyltransferase SETD2 causes transcription and alternative splicing changes resulting in oncogenic phenotypes
Authors: Bhattacharya Saikat , Reddy Divya , Zhang Ning , Li Hua , Workman Jerry L.
Published: August 2022

The methyltransferase SETD2 regulates cryptic transcription, alternative splicing, and the DNA damage response. It is mutated in a variety of cancers and is believed to be a tumor suppressor. Counterintuitively, despite its important role, SETD2 is robustly degraded by the proteasome keeping its levels low. Here we show that SETD2 accumulation results in a non-canonical deposition of the functionally important H3K36me3 histone mark, which includes its reduced enrichment over gene bodies and exons. This perturbed epigenetic landscape is associated with widespread changes in transcription and alternative splicing. Strikingly, contrary to its role as a tumor suppressor, excessive SETD2 results in the upregulation of cell cycle-associated pathways. This is also reflected in phenotypes of increased cell proliferation and migration. Thus, the regulation of SETD2 levels through its proteolysis is important to maintain its appropriate function, which in turn regulates the fidelity of transcription and splicing-related processes.
Identification of the porcine IG-DMR and abnormal imprinting of DLK1-DIO3 in cloned pigs
Authors: Li Junliang , Yu Dawei , Wang Jing , Li Chongyang , Wang Qingwei , Wang Jing , Du Weihua , Zhao Shanjiang , Pang Yunwei , Hao Haisheng , Zhao Xueming , Zhu Huabin , Li Shijie , Zou Huiying
Published: August 2022

Correct reprogramming of the DLK1-DIO3 imprinted region is critical for the development of cloned animals. However, in pigs, the imprinting and regulation of the DLK1-DIO3 region has not been systematically analyzed. The objective of this study was to investigate the imprinting status and methylation regulation of the DLK1-DIO3 region in wild-type and cloned neonatal pigs. We mapped the imprinting control region, IG-DMR, by homologous alignment and validated it in sperm, oocytes, fibroblasts, and parthenogenetic embryos. Subsequently, single nucleotide polymorphism-based sequencing and bisulfite sequencing polymerase chain reaction were conducted to analyze imprinting and methylation in different types of fibroblasts, as well as wild-type and cloned neonatal pigs. The results showed that Somatic cell nuclear transfer (SCNT) resulted in hypermethylation of the IG-DMR and aberrant gene expression in the DLK1-DIO3 region. Similar to wild-type pigs, imprinted expression and methylation were observed in the surviving cloned pigs, whereas in dead cloned pigs, the IG-DMR was hypermethylated and the expression of GTL2 was nearly undetectable. Our study reveals that abnormal imprinting of the DLK1-DIO3 region occurs in cloned pigs, which provides a theoretical basis for improving the cloning efficiency by gene editing to correct abnormal imprinting.
Continuous crystallisation of organic salt polymorphs
Authors: McGinty John , Svoboda Vaclav , MacFhionnghaile Pol , Wheatcroft Helen , Price Chris J. , Sefcik Jan
Published: August 2022

Organic salt crystallisation is of great importance to the pharmaceutical industry as many pharmaceutical products are marketed as salts with salt formation being an essential step in drug development. The model compound used in this work is the polymorphic organic salt ethylenediammonium 3,5-dinitrobenzoate (EDNB) which is the 2:1 salt of 3,5-dinitrobenzoic acid with ethylenediamine. Crystallisation of the two EDNB polymorphs, the stable monoclinic form and the metastable triclinic form, was performed in both semi-batch and continuous processes using continuous mixing approaches. It was demonstrated that continuous mixing approaches, using various types of continuous mixers (co-axial, Ehrfeld, X-mixer) can be used to crystallise the EDNB salt with consistent yield and particle size distribution while achieving control over polymorphic form. The experiments were designed with use of a solution speciation model which provided process understanding and insight to guide development of salt crystallisation processes.
Characteristics of oral microbiota in plateau and plain youth‐positive correlations between blood lipid level, metabolism and specific microflora in the plateau group
Authors: Zhao LiBo , Wang Huanhuan , Gao Yinghui , Hao Benchuan , Li Xueyan , Wen Ruoqing , Chen Kaibing , Fan Li , Liu Lin
Published: August 2022

Objectives

To analyze the characteristics of oral microbiota in plateau and plain youth and the possible function of the microbiome.

Materials and methods

A total of 120 healthy young males (80 on the plateau, 40 on the plain) completed this cross-sectional study. Oral microflora samples were collected from all participants. The bacterial 16S rDNA was amplified using PCR and sequenced using Illumina MiSeq high-throughput sequencing. The data were analyzed to determine the microbial distribution and community structure of the oral microflora from the two groups. Metastats was used to test differences in relative species abundance between the groups. The correlation between the abundance of specific bacteria and blood indicators was also analyzed.

Results

As demonstrated by alpha and beta diversity, the plateau group had lower microbial richness and a less even distribution of oral microbiota than the plain group. All predominant phyla and genera were qualitatively similar between the two groups, but their relative abundances differed. The relative abundance of bacteria in the phylum Firmicutes was significantly higher in the plateau group than in the plain group. At the genus level, Streptococcus spp. and Gemella spp. were also more abundant in the plateau group. The functional prediction indicated vigorous microbial metabolism in the oral bacterial community. We also found that the relative abundance of Streptococcus spp., the dominant genus, was positively correlated with triglyceride levels in the plateau group.

Conclusions

With increasing altitude, the diversity of oral microbiota and the relative proportion of predominant bacteria were altered. The distribution and related function of Streptococcus spp. were prominent in plateau samples. This comprehensive study of the relationship between oral microecology and elevation provides a point of reference for studying the human body’s adaptability or inadaptability to high altitude.