bluuebunny/crossref_metadata_2025 huggingface dataset:
Chaos exponents of SYK traversable wormholes
Authors: Nosaka Tomoki , Numasawa Tokiro
Published: February 2021

Abstract

In this paper we study the chaos exponent, the exponential growth rate of the out-of-time-ordered four point functions, in a two coupled SYK models which exhibits a first order phase transition between the high temperature black hole phase and the low temperature gapped phase interpreted as a traversable wormhole. We see that as the temperature decreases the chaos exponent exhibits a discontinuous fall-off from the value of order the universal bound 2π/β at the critical temperature of the phase transition, which is consistent with the expected relation between black holes and strong chaos. Interestingly, the chaos exponent is small but non-zero even in the wormhole phase. This is surprising but consistent with the observation on the decay rate of the two point function [1], and we found the chaos exponent and the decay rate indeed obey the same temperature dependence in this regime. We also studied the chaos exponent of a closely related model with single SYK term, and found that the chaos exponent of this model is always greater than that of the two coupled model in the entire parameter space.
Exotic Higgs decays into displaced jets at the LHeC
Authors: Cheung Kingman , Fischer Oliver , Wang Zeren Simon , Zurita Jose
Published: February 2021

Abstract

Profiling the Higgs boson requires the study of its non-standard decay modes. In this work we discuss the prospects of the Large Hadron electron Collider (LHeC) to detect scalar particles with masses ,≳ 10 GeV produced from decays of the Standard Model (SM) Higgs boson. These scalar particles decay mainly to bottom pairs, and in a vast portion of the allowed parameter space they acquire a macroscopic lifetime, hence giving rise to displaced hadronic vertices. The LHeC provides a very clean environment that allows for easy identification of these final states, in contrast to hadronic colliders where the overwhelming backgrounds and high pile-up render such searches incredibly challenging. We find that the LHeC provides a unique window of opportunity to detect scalar particles with masses between 10 and 30 GeV. In the Higgs Portal scenarios we can test the mixing angle squared, sin2α, as low as 10−5–10−7, with the exact value depending on the vacuum expectation value of the new scalar.

Our results are also presented in a model-independent fashion in the lifetime-branching ratio and mass-branching ratio planes. We have found that exotic branching ratios of the Higgs boson at the sub-percent level can be probed, for the scalar decay length in the range 10−4 m ≲ cτ ≲ 10−1 m. The expected coverage of the parameter space largely exceeds the published sensitivity of the indirect reach at the high-luminosity Large Hadron Collider via the invisible Higgs branching ratio.
Estimating the quantum requirements for plant growth and related electricity demand for LED lighting systems
Authors: Schmierer Marc , Brueck Holger , Asch Folkard , Sauerborn Joachim
Published: February 2021

Indoor plant production systems with artificial lighting are considered an emerging technology contributing to biomass-based value webs. The viability of this concept greatly relies on the energy requirements (ER, Watt) for lighting. We estimated the ER for plant growth by calculating the conversion efficiency of electricity to light of solid-state light-emitting diodes (LED) and the quantum requirements for plant growth of a fictional plant stand producing 2500 g of dry weight per m2 of ground during 100 days, representing a high productivity benchmark of field crops. The quantum output (µmol s−1 W−1) of eight LEDs of different colours varied between 0.78 for green and 2.54 for deep red. Uncertainty in the H+ demand for ATP synthesis during photosynthesis, the relative portion of photorespiration and the fraction of light intercepted by plant canopies (fabs) were considered in a pessimistic (PA) and optimistic (OA) approach of calculation of ER. Cumulative ER were 606 and 265 kWh m−2 for the PA and OA scenarios. The energy conversion efficiencies in the PA and OA scenarios were 2.07 and 4.72%. Estimates of energy savings by suppressing photorespiration and increasing fabs vary between 24 and 38%. The peak daily ER were 9.44 and 4.14 kWh in the PA and OA scenarios. Results are discussed in the context of the design of lighting in indoor plant production systems and commercial greenhouses where natural fluctuation in solar radiation could be balanced by dimmable LED panels.
Crosstalk between endoplasmic reticulum stress and oxidative stress: a dynamic duo in multiple myeloma
Authors: Xiong Sinan , Chng Wee-Joo , Zhou Jianbiao
Published: February 2021

Under physiological and pathological conditions, cells activate the unfolded protein response (UPR) to deal with the accumulation of unfolded or misfolded proteins in the endoplasmic reticulum. Multiple myeloma (MM) is a hematological malignancy arising from immunoglobulin-secreting plasma cells. MM cells are subject to continual ER stress and highly dependent on the UPR signaling activation due to overproduction of paraproteins. Mounting evidence suggests the close linkage between ER stress and oxidative stress, demonstrated by overlapping signaling pathways and inter-organelle communication pivotal to cell fate decision. Imbalance of intracellular homeostasis can lead to deranged control of cellular functions and engage apoptosis due to mutual activation between ER stress and reactive oxygen species generation through a self-perpetuating cycle. Here, we present accumulating evidence showing the interactive roles of redox homeostasis and proteostasis in MM pathogenesis and drug resistance, which would be helpful in elucidating the still underdefined molecular pathways linking ER stress and oxidative stress in MM. Lastly, we highlight future research directions in the development of anti-myeloma therapy, focusing particularly on targeting redox signaling and ER stress responses.
Insect antimicrobial peptides: potential weapons to counteract the antibiotic resistance
Authors: Manniello M. D. , Moretta A. , Salvia R. , Scieuzo C. , Lucchetti D. , Vogel H. , Sgambato A. , Falabella P.
Published: February 2021

Misuse and overuse of antibiotics have contributed in the last decades to a phenomenon known as antibiotic resistance which is currently considered one of the principal threats to global public health by the World Health Organization. The aim to find alternative drugs has been demonstrated as a real challenge. Thanks to their biodiversity, insects represent the largest class of organisms in the animal kingdom. The humoral immune response includes the production of antimicrobial peptides (AMPs) that are released into the insect hemolymph after microbial infection. In this review, we have focused on insect immune responses, particularly on AMP characteristics, their mechanism of action and applications, especially in the biomedical field. Furthermore, we discuss the Toll, Imd, and JAK-STAT pathways that activate genes encoding for the expression of AMPs. Moreover, we focused on strategies to improve insect peptides stability against proteolytic susceptibility such as D-amino acid substitutions, N-terminus modification, cyclization and dimerization.
An unusual thioredoxin system in the facultative parasite Acanthamoeba castellanii
Authors: Leitsch David , Mbouaka Alvie Loufouma , Köhsler Martina , Müller Norbert , Walochnik Julia
Published: February 2021

The free-living amoeba Acanthamoeba castellanii occurs worldwide in soil and water and feeds on bacteria and other microorganisms. It is, however, also a facultative parasite and can cause serious infections in humans. The annotated genome of A. castellanii (strain Neff) suggests the presence of two different thioredoxin reductases (TrxR), of which one is of the small bacterial type and the other of the large vertebrate type. This combination is highly unusual. Similar to vertebrate TrxRases, the gene coding for the large TrxR in A. castellanii contains a UGA stop codon at the C-terminal active site, suggesting the presence of selenocysteine. We characterized the thioredoxin system in A. castellanii in conjunction with glutathione reductase (GR), to obtain a more complete understanding of the redox system in A. castellanii and the roles of its components in the response to oxidative stress. Both TrxRases localize to the cytoplasm, whereas GR localizes to the cytoplasm and the large organelle fraction. We could only identify one thioredoxin (Trx-1) to be indeed reduced by one of the TrxRases, i.e., by the small TrxR. This thioredoxin, in turn, could reduce one of the two peroxiredoxins tested and also methionine sulfoxide reductase A (MsrA). Upon exposure to hydrogen peroxide and diamide, only the small TrxR was upregulated in expression at the mRNA and protein levels, but not the large TrxR. Our results show that the small TrxR is involved in the A. castellanii’s response to oxidative stress. The role of the large TrxR, however, remains elusive.
Experimental tests of PVD AlCrN-coated planer knives on planing Scots pine (Pinus sylvestris L.) under industrial conditions
Authors: Nadolny Krzysztof , Kapłonek Wojciech , Sutowska Marzena , Sutowski Paweł , Myśliński Piotr , Gilewicz Adam , Warcholiński Bogdan
Published: February 2021

Raw pine wood processing and especially its mechanical processing constitute a significant share among technological operations leading to obtaining a finished product. Stable implementation of machining operations, ensuring long-term repeatable processing results depends on many factors, such as quality and invariability of raw material, technical condition of technological equipment, adopted parameters of work, qualifications and experience of operators, as well as preparation and properties of the machining tools used. It seems that the greatest potential in the search for opportunities to increase the efficiency of machining operations has the modification of machining tools used in it. This paper presents the results of research work aimed at determining how the life of cutting tools used in planing operations of wet pine wood is affected by the application of chromium aluminum nitride (AlCrN) coating to planar industrial planing knives in the process of physical vapour deposition. For this purpose operational tests were carried out under production conditions in a medium-sized wood processing company. The study compares the effective working time, rounding radius, the profile along the knife (size of worn edge displacement, wear area of the cutting edge), selected texture parameters of the planar industrial planing knife rake face and visual analyses of cutting edge condition of AlCrN-coated planar knives and unmodified ones. The obtained experimental results showed the possibility of increasing the life of AlCrN-coated knives up to 154% compared to the results obtained with uncoated ones. The proposed modification of the operational features of the knives does not involve any changes in the technological process of planing, does not require any interference with the machining station nor its parameters, therefore enabling rapid and easy implementation into industrial practice.
Aphasien bei lakunären Hirninfarkten
Authors: Kohlhase Konstantin , Schaefer Jan Hendrik , Lapa Sriramya , Jurcoane Alina , Wagner Marlies , Hok Pavel , Kell Christian A.
Published: February 2021

Hintergrund

Aphasien gehören nicht zu den typischen klinischen Manifestationen lakunärer Hirninfarkte, sind jedoch im Rahmen seltener atypischer lakunärer Syndrome beschrieben.

Ziel der Arbeit

Beschreibung von Aphasiemustern und betroffener Fasertrakte bei lakunären Infarkten.

Material und Methoden

Fallserie von drei Patienten mit in der Magnetresonanztomographie nachgewiesenen lakunären Hirninfarkten und Aphasie. Identifikation betroffener Faserbahnen mittels Fasertraktographie der koregistrierten Schädigungsorte in Gehirnen zweier gesunder Probanden.

Ergebnisse

Radiologisch waren die Lakunen, die Aphasien hervorriefen, weit lateral im Marklager der linken Hemisphäre gelegen und befanden sich im Vergleich zu der Lakune eines nichtaphasischen Kontrollpatienten weiter rostrodorsal. Klinisch fand sich trotz Aussparung des Kortex, Thalamus und weiter Teile der Basalganglien eine leichte bis moderate nichtflüssige Aphasie mit syntaktischen Defiziten. In der Fasertraktographie zeigten die aphasischen im Vergleich zum nichtaphasischen Patienten eine stärkere Affektion der Fasern des linken Fasciculus arcuatus sowie eine Beteiligung des frontostriatalen und frontalen Aslant-Trakts.

Diskussion

Links lateral gelegene lakunäre Infarkte können durch Beteiligung sprachrelevanter Fasertrakte eine klinisch relevante Aphasie hervorrufen.
Autologe Chondrozytentransplantation nach penetrierender Messerstichverletzung am Kniegelenk
Authors: Exner Lisanne , Abdelhai Mohammed , Dalheimer Philipp
Published: February 2021

Die penetrierende Kniegelenkverletzung ist ein seltenes Trauma, welches jedoch nicht unterschätzt werden darf. In der Regel besteht die Indikation zur zeitnahen operativen Versorgung. Dieser Fallbericht handelt von einem 17-jährigen Patienten, der durch einen Messerstich eine Knorpelverletzung der lateralen Femurkondyle erlitt. Es folgte die Gelenkspülung mit Bergung des Knorpelflakes und endgültiger Versorgung mittels autologer Chondrozytentransplantation. Die Gewinnung von Knorpelzellen aus dem Flake unterblieb aufgrund des Risikos einer Keimbesiedlung sowie des Qualitätsverlustes der Chondrozyten.
Preventing NEETs during the Great Recession: the effects of mandatory activation programs for young welfare recipients
Authors: Cammeraat Emile , Jongen Egbert , Koning Pierre
Published: February 2021

We study the impact of mandatory activation programs for young welfare recipients in the Netherlands. What makes this reform unique is that it clashed head on with the Great Recession. We use differences-in-differences and data for the period 1999–2012 to estimate the effects of this reform. We find that the reform reduced the number of welfare recipients but had no effect on the number of NEETs (individuals not in employment, education or training). The absence of employment effects contrasts with previous studies on the impact of mandatory activation programs, which we argue is due to the reform taking place during a severe economic recession.
Unusual eye injury related to abusive head trauma
Authors: Eddahabi Cordula , Djalali-Talab Yassin , Banaschak Sibylle , Feld Katharina
Published: February 2021

Ophthalmological examination is an essential component in clinically diagnosing abusive head trauma (AHT). Typical of AHT injuries is retinal bleeding, with other parts of the eye sometimes also being affected. Visual impairment and blindness are long-term complications in this context. We present a case with unusual eye injuries after a diagnostically confirmed AHT.

The ophthalmological findings on admission day showed a massive subhyaloid hemorrhage at the posterior pole and extensive flame-shaped intraretinal hemorrhages accented on the right side., A centrally localized vitreous hemorrhage appeared on both sides 3 weeks later, obscuring the optic disc and macula most probably due to a breakthrough of a sub-internal limiting membrane (ILM) hemorrhage into the vitreous cavity. Follow-up 4 weeks later revealed a dense and organized vitreous hemorrhage occupying the optic disc and macula so that a pars plana vitrectomy was performed on both eyes.

The bilateral bleeding in different retinal layers, the vitreous hemorrhage and the proliferative vitreoretinal reaction (PVR) indicated massive damage caused by a significant acceleration-deceleration trauma. A two-phase vitreous hemorrhage in a child with AHT does not yet appear to have been described in the literature.
Prediction of the dose range for adverse neurological effects of amiodarone in patients from an in vitro toxicity test by in vitro–in vivo extrapolation
Authors: Algharably Engi Abd el-Hady , Di Consiglio Emma , Testai Emanuela , Kreutz Reinhold , Gundert-Remy Ursula
Published: February 2021

Amiodarone is an antiarrhythmic agent inducing adverse effects on the nervous system, among others. We applied physiologically based pharmacokinetic (PBPK) modeling combined with benchmark dose modeling to predict, based on published in vitro data, the in vivo dose of amiodarone which may lead to adverse neurological effects in patients. We performed in vitro–in vivo extrapolation (IVIVE) from concentrations measured in the cell lysate of a rat brain 3D cell model using a validated human PBPK model. Among the observed in vitro effects, inhibition of choline acetyl transferase (ChAT) was selected as a marker for neurotoxicity. By reverse dosimetry, we transformed the in vitro concentration–effect relationship into in vivo effective human doses, using the calculated in vitro area under the curve (AUC) of amiodarone as the pharmacokinetic metric. The upper benchmark dose (BMDU) was calculated and compared with clinical doses eliciting neurological adverse effects in patients. The AUCs in the in vitro brain cell culture after 14-day repeated dosing of nominal concentration equal to 1.25 and 2.5 µM amiodarone were 1.00 and 1.99 µg*h/mL, respectively. The BMDU was 385.4 mg for intravenous converted to 593 mg for oral application using the bioavailability factor of 0.65 as reported in the literature. The predicted dose compares well with neurotoxic doses in patients supporting the hypothesis that impaired ChAT activity may be related to the molecular/cellular mechanisms of amiodarone neurotoxicity. Our study shows that predicting effects from in vitro data together with IVIVE can be used at the initial stage for the evaluation of potential adverse drug reactions and safety assessment in humans.
Khovanov homotopy type, periodic links and localizations
Authors: Borodzik Maciej , Politarczyk Wojciech , Silvero Marithania
Published: February 2021

Given an m-periodic link $$L\subset S^3$$

L
⊂

S
3

, we show that the Khovanov spectrum $$\mathcal {X}_L$$

X
L

constructed by Lipshitz and Sarkar admits a group action. We relate the Borel cohomology of $$\mathcal {X}_L$$

X
L

to the equivariant Khovanov homology of L constructed by the second author. The action of Steenrod algebra on the cohomology of $$\mathcal {X}_L$$

X
L

gives an extra structure of the periodic link. Another consequence of our construction is an alternative proof of the localization formula for Khovanov homology, obtained first by Stoffregen and Zhang. By applying the Dwyer–Wilkerson theorem we express Khovanov homology of the quotient link in terms of equivariant Khovanov homology of the original link.
Solution to a problem of FitzGerald
Authors: Hemelaer Jens , Rogers Morgan
Published: February 2021

FitzGerald identified four conditions (RI), (UR), (RI*) and (UR*) that are necessarily satisfied by an algebra if its monoid of endomorphisms has commuting idempotents. We show that these conditions are not sufficient, by giving an example of an algebra satisfying the four properties, such that its monoid of endomorphisms does not have commuting idempotents. This settles a problem presented by FitzGerald at the Conference and Workshop on General Algebra and Its Applications in 2013 and more recently at the workshop NCS 2018. After giving the counterexample, we show that the properties (UR), (RI*) and (UR*) depend only on the monoid of endomorphisms of the algebra, and that the counterexample we gave is in some sense the easiest possible. Finally, we list some categories in which FitzGerald’s question has an affirmative answer.
Assessing Biotic and Abiotic Interactions of Microorganisms in Amazonia through Co-Occurrence Networks and DNA Metabarcoding
Authors: Ritter Camila Duarte , Forster Dominik , Azevedo Josue A. R. , Antonelli Alexandre , Nilsson R. Henrik , Trujillo Martha E. , Dunthorn Micah
Published: February 2021

Species may co-occur due to responses to similar environmental conditions, biological associations, or simply because of coincident geographical distributions. Disentangling patterns of co-occurrence and potential biotic and abiotic interactions is crucial to understand ecosystem function. Here, we used DNA metabarcoding data from litter and mineral soils collected from a longitudinal transect in Amazonia to explore patterns of co-occurrence. We compared data from different Amazonian habitat types, each with a characteristic biota and environmental conditions. These included non-flooded rainforests (terra-firme), forests seasonally flooded by fertile white waters (várzeas) or by unfertile black waters (igapós), and open areas associated with white sand soil (campinas). We ran co-occurrence network analyses based on null models and Spearman correlation for all samples and for each habitat separately. We found that one third of all operational taxonomic units (OTUs) were bacteria and two thirds were eukaryotes. The resulting networks were nevertheless mostly composed of bacteria, with fewer fungi, protists, and metazoans. Considering the functional traits of the OTUs, there is a combination of metabolism modes including respiration and fermentation for bacteria, and a high frequency of saprotrophic fungi (those that feed on dead organic matter), indicating a high turnover of organic material. The organic carbon and base saturation indices were important in the co-occurrences in Amazonian networks, whereas several other soil properties were important for the co-exclusion. Different habitats had similar network properties with some variation in terms of modularity, probably associated with flooding pulse. We show that Amazonian microorganism communities form highly interconnected co-occurrence and co-exclusion networks, which highlights the importance of complex biotic and abiotic interactions in explaining the outstanding biodiversity of the region.
Outcomes of clinical utility in amyloid-PET studies: state of art and future perspectives
Authors: Cotta Ramusino Matteo , Perini Giulia , Altomare Daniele , Barbarino Paola , Weidner Wendy , Salvini Porro Gabriella , Barkhof Frederik , Rabinovici Gil D. , van der Flier Wiesje M. , Frisoni Giovanni B. , Garibotto Valentina , Teipel Stefan , Boccardi Marina
Published: February 2021

Purpose

To review how outcomes of clinical utility are operationalized in current amyloid-PET validation studies, to prepare for formal assessment of clinical utility of amyloid-PET-based diagnosis.

Methods

Systematic review of amyloid-PET research studies published up to April 2020 that included outcomes of clinical utility. We extracted and analyzed (a) outcome categories, (b) their definition, and (c) their methods of assessment.

Results

Thirty-two studies were eligible. (a) Outcome categories were clinician-centered (found in 25/32 studies, 78%), patient-/caregiver-centered (in 9/32 studies, 28%), and health economics-centered (5/32, 16%). (b) Definition: Outcomes were mainly defined by clinical researchers; only the ABIDE study expressly included stakeholders in group discussions. Clinician-centered outcomes mainly consisted of incremental diagnostic value (25/32, 78%) and change in patient management (17/32, 53%); patient-/caregiver-centered outcomes considered distress after amyloid-pet-based diagnosis disclosure (8/32, 25%), including quantified burden of procedure for patients’ outcomes (n = 8) (1/8, 12.5%), impact of disclosure of results (6/8, 75%), and psychological implications of biomarker-based diagnosis (75%); and health economics outcomes focused on costs to achieve a high-confidence etiological diagnosis (5/32, 16%) and impact on quality of life (1/32, 3%). (c) Assessment: all outcome categories were operationalized inconsistently across studies, employing 26 different tools without formal rationale for selection.

Conclusion

Current studies validating amyloid-PET already assessed outcomes for clinical utility, although non-clinician-based outcomes were inconsistent. A wider participation of stakeholders may help produce a more thorough and systematic definition and assessment of outcomes of clinical utility and help collect evidence informing decisions on reimbursement of amyloid-PET.
Multi-scale optoacoustic molecular imaging of brain diseases
Authors: Razansky Daniel , Klohs Jan , Ni Ruiqing
Published: February 2021

The ability to non-invasively visualize endogenous chromophores and exogenous probes and sensors across the entire rodent brain with the high spatial and temporal resolution has empowered optoacoustic imaging modalities with unprecedented capacities for interrogating the brain under physiological and diseased conditions. This has rapidly transformed optoacoustic microscopy (OAM) and multi-spectral optoacoustic tomography (MSOT) into emerging research tools to study animal models of brain diseases. In this review, we describe the principles of optoacoustic imaging and showcase recent technical advances that enable high-resolution real-time brain observations in preclinical models. In addition, advanced molecular probe designs allow for efficient visualization of pathophysiological processes playing a central role in a variety of neurodegenerative diseases, brain tumors, and stroke. We describe outstanding challenges in optoacoustic imaging methodologies and propose a future outlook.
Surgical Outcome of Pancreaticoduodenectomy in Patients with a History of Roux‐en‐Y Reconstruction after Tumor Removal
Authors: Marukawa Daiki , Tomimaru Yoshito , Kobayashi Shogo , Iwagami Yoshifumi , Yamada Daisaku , Akita Hirofumi , Noda Takehiro , Gotoh Kunihito , Doki Yuichiro , Eguchi Hidetoshi
Published: February 2021

Background

The need for pancreaticoduodenectomy (PD) after Roux‐en‐Y (RY) reconstruction after tumor removal is expected to increase in future, but current studies on outcome are sparse. This surgery is challenging, due to intraabdominal adhesions and/or anatomical changes introduced by the previous abdominal surgery. Here, we investigated the surgical outcomes of PD after RY reconstruction following tumor removal.

Methods

We enrolled 283 patients that underwent PD. Surgical outcomes for PD were compared between patients with or without a history of RY reconstruction after tumor removal. Outcomes were also compared between two different surgical procedures for the post‐PD reconstruction.

Results

Among 283 patients, 11 had a history of RY reconstruction after tumor removal (3.9%). Among these, RY reconstructions had been performed where the small intestine was anastomosed to a remnant stomach after distal gastrectomy (n = 2), to remnant stomach after proximal gastrectomy (n = 1), to the esophagus after total gastrectomy (n = 6), or to the hepatic duct after extrahepatic bile duct resection (n = 2). Surgical outcomes were not significantly different between cases with and without RY reconstructions. We identified two different reconstruction procedures after removing the periampullary tumor during PD. The surgical outcomes were not significantly different between these two reconstruction groups.

Conclusions

The surgical outcome of PD was not significantly affected by a history of RY reconstruction. Similarly, the type of reconstruction performed during PD did not significantly affect the outcome. These results could be useful when planning PD in patients with a history of RY reconstruction after tumor removal.
Validation of the Surgical Outcome Risk Tool (SORT) for Predicting Postoperative Mortality in Colorectal Cancer Patients Undergoing Surgery and Subgroup Analysis
Authors: Magouliotis Dimitrios E. , Walker David , Baloyiannis Ioannis , Fergadi Maria P. , Mamaloudis Ioannis , Chasiotis Georgios , Tzovaras George A.
Published: February 2021

Background

The accurate evaluation of perioperative risk is crucial to facilitate the shared decision‐making process. Surgical outcome risk tool (SORT) has been developed to provide enhanced and more feasible identification of high‐risk surgical patients. Nonetheless, SORT has not been validated for patients with colorectal cancer undergoing surgery. Our aim was to determine whether SORT can accurately predict mortality after surgery for colorectal cancer and to compare it with traditional risk models.

Method

526 patients undergoing surgery performed by a colorectal surgical team in a single Greek tertiary hospital (2011–2019) were included. Five risk models were evaluated: (1) SORT, (2) Physiology and Operative Severity Score for the enumeration of Mortality and Morbidity (POSSUM), (3) Portsmouth POSSUM (P‐POSSUM), (4) Colorectal POSSUM (CR‐POSSUM), and (5) the Association of Great Britain and Ireland (ACPGBI) score. Model accuracy was assessed by observed to expected (O:E) ratios, and area under Receiver Operating Characteristic curve (AUC).

Results

Ten patients (1.9%) died within 30 days of surgery. SORT was associated with an excellent level of discrimination [AUC:0.81 (95% CI:0.68–0.94); p = 0.001] and provided the best performing calibration of all models in the entire dataset analysis (H–L:2.82; p = 0.83). Nonetheless, SORT underestimated mortality. SORT model demonstrated excellent discrimination and calibration predicting perioperative mortality in patients undergoing (1) open surgery, (2) emergency/acute surgery, and (3) in cases with colon‐located cancer.

Conclusion

SORT is an easily adopted risk‐assessment tool, associated with enhanced accuracy, that could be implemented in the perioperative pathway of patients undergoing surgery for colorectal cancer.
Mono- and multispecies biofilms from a crustose coralline alga induce settlement in the scleractinian coral Leptastrea purpurea
Authors: Petersen Lars-Erik , Moeller Mareen , Versluis Dennis , Nietzer Samuel , Kellermann Matthias Y. , Schupp Peter J.
Published: February 2021

Microorganisms have been reported to induce settlement in various marine invertebrate larvae but their specificity of inductive capacities for the settlement of coral larvae remains poorly understood. In this study, we isolated 56 microbial strains from the crustose coralline alga (CCA) Hydrolithon reinboldii using five different media either with or without additional antibiotics and/or spiked CCA extract. We tested the isolates for their potential to induce settlement behavior in larvae of the brooding scleractinian coral Leptastrea purpurea. From these 56 CCA-associated microbial strains, we identified six bacterial classes and 18 families. The culturable bacterial community associated with H. reinboldii was dominated by Gammaproteobacteria, Actinobacteria, and Alphaproteobacteria while the Illumina MiSeq analysis showed that the culture-independent bacterial community was dominated by Gammaproteobacteria, Alphaproteobacteria, and Flavobacteria. Furthermore, we found no correlation between inductive settlement capacities and phylogenetic relationships. Instead, settlement behavior of L. purpurea larvae was induced by specific isolated species. Strains #1792 (Pseudovibrio denitrificans), #1678 (Acinetobacter pittii), #1633 (Pseudoalteromonas phenolica), #1772 (Marine bacterium LMG1), #1721 (Microbulbifer variabilis), and #1783 (Pseudoalteromonas rubra) induced settlement behavior in coral larvae at mostly high and significant levels (≥ 40%) but the remaining isolates strongly varied in their activity. Multispecies biofilms consisting of four strains (#1792, #1678, #1633, and #1721) were observed to synergistically increase settlement behavior levels (> 90%); however, the addition of #1772 to the multispecies biofilms negatively affected coral larvae and resulted in a total loss of inducing activity. The findings provide new insights into the role of bacteria in the settlement process of scleractinian corals and may help to identify the true nature of bacteria-derived morphogenic cues.
Effect of the air–sea coupled system change on the ENSO evolution from boreal spring
Authors: Fang Xiang-Hui , Zheng Fei
Published: February 2021

Realistic simulation and accurate prediction of El Niño-Southern Oscillation (ENSO) is still a challenge. One fundamental obstacle is the so-called spring predictability barrier (SPB), which features a low predictive skill of the ENSO with prediction across boreal spring. Our observational analysis shows that the leading empirical orthogonal function mode of the seasonal Niño3.4 index evolution (i.e., from May to the following April) explains nearly 90% of its total variance, and the principle component is almost identical to the Niño3.4 index in the mature phase. This means a good ENSO prediction for a year ranging May-next April can be achieved if the Niño3.4 index in the mature phase is accurately obtained in advance. In this work, by extracting physically oriented variables in the spring, a linear regression approach that can reproduce the mature ENSO phases in observation is firstly proposed. Further investigation indicates that the specific equation, however, is significantly modulated by an interdecadal regime shift in the air–sea coupled system in the tropical Pacific. During 1980–1999, ocean adjustment and vertical processes were dominant, and the recharge oscillator theory was effective to capture the ENSO evolutions. While, during 2000–2018, zonal advection and thermodynamics became important, and successful prediction essentially relies on the wind stress information and their controlled processes, both zonally and meridionally. These results imply that accounting for the interdecadal regime shift of the tropical Pacific coupled system and the dominant processes in spring in modulating the ENSO evolution could reduce the impact of SPB and improve ENSO prediction.
Molecular clocks in ancient proteins: Do they reflect the age at death even after millennia?
Authors: Mahlke Nina Sophia , Renhart Silvia , Talaa Dorothea , Reckert Alexandra , Ritz-Timme Stefanie
Published: February 2021

Age at death estimation in cases of human skeletal finds is an important task in forensic medicine as well as in anthropology. In forensic medicine, methods based on “molecular clocks” in dental tissues and bone play an increasing role. The question, whether these methods are applicable also in cases with post-depositional intervals far beyond the forensically relevant period, was investigated for two “protein clocks”, the accumulation of D-aspartic acid (D-Asp) and the accumulation of pentosidine (Pen) in dentine. Eight teeth of skeletons from different burial sites in Austria and with post-depositional intervals between c. 1216 and c. 8775 years were analysed. The results of age at death estimation based on D-Asp and Pen in dentine were compared to that derived from a classical morphological examination. Age at death estimation based on D-Asp resulted consistently in false high values. This finding can be explained by a post-mortem accumulation of D-Asp that may be enhanced by protein degradation. In contrast, the Pen-based age estimates fitted well with the morphological age diagnoses. The described effect of post-mortem protein degradation is negligible in forensically relevant time horizons, but not for post-depositional intervals of thousands of years. That means that the “D-Asp clock” loses its functionality with increasing post-depositional intervals, whereas Pen seems to be very stable. The “Pen-clock” may have the potential to become an interesting supplement to the existing repertoire of methods even in cases with extremely long post-depositional intervals. Further investigations have to test this hypothesis.
Modulation of intracortical inhibition during physically performed and mentally simulated balance tasks
Authors: Mouthon A. , Ruffieux J. , Taube W.
Published: February 2021

Purpose

Action observation (AO) during motor imagery (MI), so-called AO + MI, has been proposed as a new form of non-physical training, but the neural mechanisms involved remains largely unknown. Therefore, this study aimed to explore whether there were similarities in the modulation of short-interval intracortical inhibition (SICI) during execution and mental simulation of postural tasks, and if there was a difference in modulation of SICI between AO + MI and AO alone.

Method

21 young adults (mean ± SD = 24 ± 6.3 years) were asked to either passively observe (AO) or imagine while observing (AO + MI) or physically perform a stable and an unstable standing task, while motor evoked potentials and SICI were assessed in the soleus muscle.

Result

SICI results showed a modulation by condition (F2,40 = 6.42, p = 0.009) with less SICI in the execution condition compared to the AO + MI (p = 0.009) and AO (p = 0.002) condition. Moreover, switching from the stable to the unstable stance condition reduced significantly SICI (F1,20 = 8.34, p = 0.009) during both, physically performed (− 38.5%; p = 0.03) and mentally simulated balance (− 10%, p < 0.001, AO + MI and AO taken together).

Conclusion

The data demonstrate that SICI is reduced when switching from a stable to a more unstable standing task during both real task execution and mental simulation. Therefore, our results strengthen and further support the existence of similarities between executed and mentally simulated actions by showing that not only corticospinal excitability is similarly modulated but also SICI. This proposes that the activity of the inhibitory cortical network during mental simulation of balance tasks resembles the one during physical postural task execution.
Canine DVL2 variant contributes to brachycephalic phenotype and caudal vertebral anomalies
Authors: Niskanen Julia E. , Reunanen Vilma , Salonen Milla , Bannasch Danika , Lappalainen Anu K. , Lohi Hannes , Hytönen Marjo K.
Published: February 2021

A frameshift deletion variant in the Wnt pathway gene dishevelled 2 (DVL2) is associated with a truncated, kinked tail (“screw tail”) in English Bulldogs, French Bulldogs and Boston Terriers. These breeds are also characterized by distinctive morphological traits, including a wide head, flat face and short-limbed dwarfism, which are characteristic of Robinow syndrome in humans, caused by defects in genes such as DVL1 and DVL3. Based on these phenotypic and genetic similarities, it has previously been hypothesized that the canine DVL2 variant results in a syndromic phenotype called the Robinow-like syndrome. In our study, we investigated the distribution of the DVL2 variant in 1954 dogs from 15 breeds, identifying breeds with allele variation and enabling the dissection of the genotype–phenotype correlation for the first time. With CT examinations in American Staffordshire Terriers, we confirmed that the DVL2 allele is associated with caudal vertebral malformations and a brachycephalic phenotype. We also hypothesize that the variant may be linked to additional health conditions, including brachycephalic obstructive airway syndrome and congenital heart defects. Altogether, our study strengthens the role of DVL2 as one of the contributors to the “bulldog type” morphology and features on the spectrum of human Robinow syndrome.
A missense variant in IFT122 associated with a canine model of retinitis pigmentosa
Authors: Kaukonen Maria , Pettinen Inka-Tuulevi , Wickström Kaisa , Arumilli Meharji , Donner Jonas , Juhola Ida-Julia , Holopainen Saila , Turunen Joni A. , Yoshihara Masahito , Kere Juha , Lohi Hannes
Published: February 2021

Retinitis pigmentosa (RP) is a blinding eye disease affecting nearly two million people worldwide. Dogs are affected with a similar illness termed progressive retinal atrophy (PRA). Lapponian herders (LHs) are affected with several types of inherited retinal dystrophies, and variants inPRCDandBEST1genes have been associated with generalized PRA and canine multifocal retinopathy 3 (cmr3), respectively. However, all retinal dystrophy cases in LHs are not explained by these variants, indicating additional genetic causes of disease in the breed. We collected DNA samples from 10 PRA affected LHs, with knownPRCDandBEST1variants excluded, and 34 unaffected LHs. A genome-wide association study identified a locus on CFA20 (praw = 2.4 × 10–7,pBonf = 0.035), and subsequent whole-genome sequencing of an affected LH revealed a missense variant, c.3176G>A, in the intraflagellar transport 122 (IFT122) gene. The variant was also found in Finnish Lapphunds, in which its clinical relevancy needs to be studied further. The variant interrupts a highly conserved residue, p.(R1059H), in IFT122 and likely impairs its function. Variants inIFT122have not been associated with retinal degeneration in mammals, but the loss of ift122 in zebrafish larvae impaired opsin transport and resulted in progressive photoreceptor degeneration. Our study establishes a new spontaneous dog model to study the role of IFT122 in RP biology, while the affected breed will benefit from a genetic test for a recessive condition.
Mixed Consistencies in Dysphagic Patients: A Myth to Dispel
Authors: Francesco Mozzanica , Nicole Pizzorni , Letizia Scarponi , Claudia Bazzotti , Daniela Ginocchio , Antonio Schindler
Published: February 2021

Only limited and inconsistent information about the effect of mixed consistencies on swallowing are available. The aim of this study was to evaluate the location of the head of the bolus at the swallow onset, the risk of penetration/aspiration, and the severity of post-swallow pharyngeal residue in patients with dysphagia when consuming mixed consistencies. 20 dysphagic patients underwent a Fiberoptic Endoscopic Evaluation of Swallowing (FEES) testing five different textures: liquid, semisolid, solid, biscuits-with-milk and vegetable-soup. The location of the head of the bolus at the onset of swallowing was rated using a five-points scale ranging from zero (the bolus is behind the tongue) to four (the bolus falls into the laryngeal vestibule), the severity of penetration/aspiration was rated using the Penetration Aspiration Scale (PAS), the amount of pharyngeal residue after the swallow was rated using the Yale Pharyngeal Residue Severity Rating Scale (YPRSRS) in the vallecula and pyriform sinus. When consuming biscuits-with-milk and liquid the swallow onset occurred more often when the boluses were located in the laryngeal vestibule. Penetration was more frequent with biscuits-with-milk, while aspiration was more frequent with Liquid, followed by biscuits-with-milk and vegetable-soup, Semisolid and Solid. In particular, no differences in penetration and aspiration between liquids and biscuits-with-milk were found as well as among vegetable-soup, semisolid and solid. No significant differences in the amount of food residue after swallowing were demonstrated. The risk of penetration-aspiration for biscuits-with-milk and liquid is similar, while the risk of penetration-aspiration is lower for vegetable-soup than for liquid.
IWATE criteria are associated with perioperative outcomes in robotic hepatectomy: a retrospective review of 225 resections
Authors: Labadie Kevin P. , Droullard David J. , Lois Alex W. , Daniel Sara K. , McNevin Kathryn E. , Gonzalez Jaqueline Valdez , Seo Yongwoo D. , Sullivan Kevin M. , Bilodeau Kyle S. , Dickerson Lindsay K. , Utria Alan F. , Calhoun John , Pillarisetty Venu G. , Sham Jonathan G. , Yeung Raymond S. , Park James O.
Published: February 2021

Background

Robotic hepatectomy (RH) is increasingly utilized for minor and major liver resections. The IWATE criteria were developed to classify minimally invasive liver resections by difficulty. The objective of this study was to apply the IWATE criteria in RH and to describe perioperative and oncologic outcomes of RH over the last decade at our institution.

Methods

Perioperative and oncologic outcomes of patients who underwent RH between 2011 and 2019 were retrospectively collected. The difficulty level of each operation was assessed using the IWATE criteria, and outcomes were compared at each level. Univariate linear regression was performed to characterize the relationship between IWATE criteria and perioperative outcomes (OR time, EBL, and LOS), and a multivariable model was also developed to address potential confounding by patient characteristics (age, sex, BMI, prior abdominal surgery, ASA class, and simultaneous non-hepatectomy operation).

Results

Two hundred and twenty-five RH were performed. Median IWATE criteria for RH were 6 (IQR 5–9), with low, intermediate, advanced, and expert resections accounting for 23% (n = 51), 34% (n = 77), 32% (n = 72), and 11% (n = 25) of resections, respectively. The majority of resections were parenchymal-sparing approaches, including anatomic segmentectomies and non-anatomic partial resections. 30-day complication rate was 14%, conversion to open surgery occurred in 9 patients (4%), and there were no deaths within 30 days postoperatively. In the univariate linear regression analysis, IWATE criteria were positively associated with OR time, EBL, and LOS. In the multivariable model, IWATE criteria were independently associated with greater OR time, EBL, and LOS. Two-year overall survival for hepatocellular carcinoma and intrahepatic cholangiocarcinoma was 94% and 50%, respectively.

Conclusion

In conclusion, the IWATE criteria are associated with surgical outcomes after RH. This series highlights the utility of RH for difficult hepatic resections, particularly parenchymal-sparing resections in the posterosuperior sector, extending the indication of minimally invasive hepatectomy in experienced hands and potentially offering select patients an alternative to open hepatectomy or other less definitive liver-directed treatment options.
Über die Rolle von Eisen bei multipler Sklerose
Authors: Hametner Simon
Published: February 2021

In den letzten Jahren kam es zu einer vermehrten Beforschung von Eisen im Gehirn von Personen mit multipler Sklerose (MS). Neue Techniken der Magnetresonanztomographie ermöglichen eine hochauflösende, sensitive und nichtinvasive Eisendarstellung des Gehirns. Deren Anwendung in Kombination mit histologischer Eisendarstellung führte zur Entdeckung von ringförmigen Eisenablagerungen am Rand von MS-Läsionen. Diese Eisenringe zeigen ein Fehlen von Reparaturmechanismen in chronischen MS-Läsionen an. Das Fehlen von solchen Eisenringläsionen in manchen Personen mit MS könnte auf einen etwas günstigeren Krankheitsverlauf hinweisen.
Mental health and use of health care services in opioid-exposed school-aged children compared to foster children
Authors: Sarfi Monica , Eikemo Marie , Welle-Strand Gabrielle K. , Muller Ashley Elizabeth , Lehmann Stine
Published: February 2021

Given the concerns raised regarding the effects of prenatal exposure to methadone and buprenorphine on the developmental outcomes of the children, this study assessed mental health and use of services in a national sample of school-aged children (N = 78) born to women enrolled in opioid maintenance treatment during pregnancy, compared with a group of foster children (N = 140). The majority of the opioid-exposed children lived with their birth parent(s) at the time of assessment (N = 62), while 16 lived in foster homes. Caregivers completed the Strengths and Difficulties Questionnaire (SDQ) and the Reactive Attachment Disorder scale. Teachers completed the SDQ. Three kinds of services were included in measuring service use: school-based education services, child mental health services, and hospital-based habilitation services. The main finding of the study is that children prenatally exposed to methadone or buprenorphine living with their family of origin had significantly better mental health status than their foster-placed counterparts and that of the comparison group of foster children. In addition, the exposed children living at home had less child welfare involvement, and only half of them were using any of the three services measured. The odds for using services increased significantly in accordance with increasing mental health problems, independent of group affiliation, indicating a need-based access to services. In line with other studies, we found that the odds for using one or more services was 2.3 times greater for boys than for girls. Our results contribute to a more-nuanced understanding of the developmental outcomes of prenatal exposure to methadone and buprenorphine, and factors associated with increased service use in groups of at-risk children.