bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative transcriptome analysis between inbred lines and hybrids provides molecular insights into K+ content heterosis of tobacco (Nicotiana tabacum L.)
Authors: Mo Zejun , Luo Wen , Pi Kai , Duan Lili , Wang Pingsong , Ke Yuzhou , Zeng Shuaibo , Jia Rongli , Liang Ting , Huang Ying , Liu Renxiang
Published: August 2022

Potassium (K+) is essential for crop growth. Increasing the K+ content can often directly promote the improvement of crop yield and quality. Heterosis plays an important role in genetic improvement and leads to genetic gains. We found that the K+ content of tobacco showed significant heterosis, which is highly significant for cultivating tobacco varieties with high K+ content. However, the mechanism by which K+ content heterosis occurs in tobacco leaves is not clear. In this study, a comprehensive comparative transcriptome sequencing analysis of root samples from the hybrid G70 × GDH11 and its parental inbred lines G70 and GDH11 was performed to elucidate the importance of the root uptake capacity of K+ in the formation of heterosis. The results showed that 29.53% and 60.49% of the differentially expressed genes (DEGs) exhibited dominant and over-dominant expression patterns, respectively. These non-additive upregulated DEGs were significantly enriched in GO terms, such as metal ion transport and reaction, ion balance and homeostasis, ion channel activity, root meristem growth, and regulation of root hairs. The KEGG annotation results indicated that these genes were mainly involved in the pathways such as energy metabolism, carbohydrate formation, amino acid metabolism, and signal transduction. Further analysis showed that probable potassium transporter 17 (NtKT17) and potassium transporter 5-like (NtKT5), associated with potassium ion absorption, glutamate receptor 2.2-like and glutamate receptor 2.8-like, associated with ion channel activity, LOC107782957, protein detoxification 42-like, and probable glutamate carboxypeptidase 2, associated with root configuration, showed a significantly higher expression in the hybrids. These results indicated that the over-dominant expression pattern of DEGs played a key role in the heterosis of K+ content in tobacco leaves, and the overexpression of the genes related to K+ uptake, transport, and root development in hybrids helped to improve the K+ content of plants, thus showing the phenomenon of heterosis.
Canopy nitrogen addition enhance the photosynthetic rate of canopy species by improving leaf hydraulic conductivity in a subtropical forest
Authors: Wu Guilin , Chen Dexiang , Zhou Zhang , Ye Qing , Wu Jianhui
Published: August 2022

Elucidating the effects of atmospheric nitrogen (N) deposition on the photosynthetic capacity of plants is critical to understand forest growth and conservation under global change. However, studies on this topic generally consider only understory N addition, which ignores the effect of canopy interception. In this study, we conducted a field experiment in a subtropical forest to compare the effects of canopy vs. understory N addition on the photosynthetic rate of canopy and understory species. We found that canopy N addition enhanced the photosynthetic rate of canopy species by increasing leaf hydraulic conductivity and shortening the distance of CO2 transportation. In contrast, understory N addition had non-significant effects on the photosynthetic rate of canopy species. Moreover, the photosynthetic rate of understory species was not affected by canopy or understory N addition. Interestingly, changes in hydraulic conductivity contributed more to accelerating the photosynthetic rate than changes in CO2 transport distance. Our results provide important insights into the dissimilar effects of canopy and understory N addition on the photosynthetic rates of species in subtropical forests. Based on our findings, we highlighted the urgent need to consider canopy processes in future studies on N deposition.
Using brefeldin A to disrupt cell wall polysaccharide components in rice and nitric oxide to modify cell wall structure to change aluminum tolerance
Authors: Yan Jianchao , Zhu Jiandong , Zhou Jun , Xing Chenghua , Song Hongming , Wu Kun , Cai Miaozhen
Published: August 2022

The components and structure of cell wall are closely correlated with aluminum (Al) toxicity and tolerance for plants. However, the cell wall assembly and function construction in response to Al is not known. Brefeldin A (BFA), a macrolide, is used to disrupt cell wall polysaccharide components, and nitric oxide (NO), a signal molecule, is used to modify the cell wall structure. Pretreatment with BFA accelerated Al accumulation in root tips and Al-induced inhibition of root growth of two rice genotypes of Nipponbare and Zhefu 802, and significantly decreased the cell wall polysaccharide content including pectin, hemicellulose 1, and hemicellulose 2, indicating that BFA inhibits the biosynthesis of components in the cell wall and makes the root cell wall lose the ability to resist Al. The addition of NO donor (SNP) significantly alleviated the toxic effects of Al on root growth, Al accumulation, and oxidative damage, and decreased the content of pectin polysaccharide and functional groups of hydroxyl, carboxyl, and amino in the cell wall via FTIR analysis, while had no significant effect on hemicellulose 1 and hemicellulose 2 content compared with Al treatment. Furthermore, NO didn't change the inhibition effect of BFA-induced cell wall polysaccharide biosynthesis and root growth. Taken together, BFA disrupts the integrity of cell wall and NO modifies partial cell wall composition and their functional groups, which change the Al tolerance in rice.
Thinning can increase shrub diversity and decrease herb diversity by regulating light and soil environments
Authors: Yu Jiatong , Zhang Xinna , Xu Chengyang , Hao Minhui , Choe CholHo , He Huaijiang
Published: August 2022

Tree thinning affects the light environment, which in turn affects the growth and survival of understory vegetation, thus improving species diversity and nutrient cycling, as well as the ecological habitat factors. However, the response of understory vegetation to the thinning intensity and short-time effects in the temperate broadleaf-conifer mixed forest is not completely clear. In this study, four permanent plots with a total area of 4 hm2 were established in a mixed broadleaf-conifer forest in northeast China, with thinning intensities of 20% (light thinning, LT), 35% (medium thinning, MT), 55% (heavy thinning, HT) and the unthinned plot (CK), respectively, in accordance with the basal area. The responses of species diversity to changes in understory vegetation were conducted by a structural equation model (SEM). The results showed that compared with CK, thinning significantly increased the photosynthetically active radiation (PAR) and the light quality (R/FR) (p < 0.05), while decreased the contents of soil total nitrogen (TN), total phosphorous (TP), organic matter (OM), nitrate nitrogen (NN), ammonia nitrogen (AN) and pH. The degree of fragmentation of light factors among the treatment plots gradually decreased as thinning intensity increased. Among all the thinning treatments, PAR and R/FR were found to be the optimal light condition when the forest thinning intensity was 55%. The light condition was found to have a significant negative correlation with soil TN, TP, OM, and AN. While the soil nutrients were positively correlated with herbaceous layer diversity but negatively correlated with shrub layer diversity. The soil nutrients were lost after thinning in a short time and herb diversity decreased, but shrub diversity increased significantly compared with unthinned plots. For the understory vegetation, the species diversity of shrub and herb layer were showed to be more sensitive to soil nutrients than light environment.
An expanded role for the transcription factor WRINKLED1 in the biosynthesis of triacylglycerols during seed development
Authors: Kuczynski Cathleen , McCorkle Sean , Keereetaweep Jantana , Shanklin John , Schwender Jorg
Published: August 2022

The transcription factor WRINKLED1 (WRI1) is known as a master regulator of fatty acid synthesis in developing oilseeds of Arabidopsis thaliana and other species. WRI1 is known to directly stimulate the expression of many fatty acid biosynthetic enzymes and a few targets in the lower part of the glycolytic pathway. However, it remains unclear to what extent and how the conversion of sugars into fatty acid biosynthetic precursors is controlled by WRI1. To shortlist possible gene targets for future in-planta experimental validation, here we present a strategy that combines phylogenetic foot printing of cis-regulatory elements with additional layers of evidence. Upstream regions of protein-encoding genes in A. thaliana were searched for the previously described DNA-binding consensus for WRI1, the ASML1/WRI1 (AW)-box. For about 900 genes, AW-box sites were found to be conserved across orthologous upstream regions in 11 related species of the crucifer family. For 145 select potential target genes identified this way, affinity of upstream AW-box sequences to WRI1 was assayed by Microscale Thermophoresis. This allowed definition of a refined WRI1 DNA-binding consensus. We find that known WRI1 gene targets are predictable with good confidence when upstream AW-sites are phylogenetically conserved, specifically binding WRI1 in the in vitro assay, positioned in proximity to the transcriptional start site, and if the gene is co-expressed with WRI1 during seed development. When targets predicted in this way are mapped to central metabolism, a conserved regulatory blueprint emerges that infers concerted control of contiguous pathway sections in glycolysis and fatty acid biosynthesis by WRI1. Several of the newly predicted targets are in the upper glycolysis pathway and the pentose phosphate pathway. Of these, plastidic isoforms of fructokinase (FRK3) and of phosphoglucose isomerase (PGI1) are particularly corroborated by previously reported seed phenotypes of respective null mutations.
How to capitalize on investors by using information presentation and feedback on crowdfunding projects
Authors: Wu Zhaoxiang , Yan Shaojun , Dai Jilin
Published: August 2022

As an innovative financing activity, online crowdfunding is characterized by extremely high information asymmetry. To reduce this information asymmetry, crowdfunding companies typically use information presentation, feedback, and other means to convey more information about the fundraising project to investors. Whether the information presentation and feedback affect the investment behavior of nonprofessional ordinary investors is yet to be determined. Moreover, the method by which the information presentation and feedback influence the investment behavior and consequently, the financing performance of crowdfunding companies, has to be identified as well. Currently, research on this subject remains deficient. Therefore, with signal theory and the difference in the cost of information transmission considered, this study classifies the information released by fundraisers on the crowdfunding platform into two categories: low-quality signal and high-quality signal. Projects on the JD.com Crowdfunding website are then used as research samples to explore how the difference in signal quality in the information presentation and feedback of crowdfunding projects influences financing performance from the perspective of investors. The results show that low-quality signals such as video duration, the number of updates, and the number of comments on projects positively affect the success of crowdfunding; meanwhile, crowdfunding experience, which represents high-quality signals, positively moderates the relationship between project video duration, project updates, and crowdfunding success.
Policy Intervention and Financial Sustainability in an Emerging Economy: A Structural Vector Auto Regression Analysis
Authors: Ahmed Sarah , Ellahi Nazima , Waheed Ajmal , Aman Nida
Published: August 2022

The purpose of the study is to observe the impact of policy intervention on financial sustainability using the structural vector autoregression (SVAR) analysis. The population of the study is the manufacturing sector of Pakistan, which is an emerging economy. Data for 249 firms operating in the manufacturing sector are taken, collected from Datastream from 2005 to 2019, with total observations of 2,400. To conduct the analysis, R software is used for its better visualization. Results show that firm performance, corporate governance, and sectoral policies have a positive and long-term impact on financial sustainability, whereas earning management and financialization not only have a negative impact, but this impact affects the operations of the corporate for a longer period. This study would be helpful for policymakers as it gives a framework for financial sustainability based on the policies and strategies developed by the sector.
Who made the paintings: Artists or artificial intelligence? The effects of identity on liking and purchase intention
Authors: Gu Li , Li Yong
Published: August 2022

Investigating how people respond to and view AI-created artworks is becoming increasingly crucial as the technology’s current application spreads due to its affordability and accessibility. This study examined how AI art alters people’s evaluation, purchase intention, and collection intention toward Chinese-style and Western-style paintings, and whether art expertise plays a role. Study 1 recruited participants without professional art experience (non-experts) and found that those who made the paintings would not change their liking rating, purchase intention, and collection intention. In addition, they showed ingroup preference, favoring Chinese-style relative to Western-style paintings, in line with previous evidence on cultural preference in empirical aesthetics. Study 2 further investigated the modulation effect of art expertise. Art experts evaluated less favorably (less liking, lower purchase, and collection intentions) AI-generated paintings relative to artist-made paintings, while non-experts showed no preference. There was also an interaction effect between the author and the art expertise and interaction between the painting style and the art expertise. Collectively, the findings in this study showed that who made the art matters for experts and that the painting style affects aesthetic evaluation and ultimate reception of it. These results would also provide implications for AI-art practitioners.
Influencing factors for effective teaching evaluation of massively open online courses in the COVID-19 epidemics: An exploratory study based on grounded theory
Authors: Liu Jingkuang , Yi Yanqing , Wang Xuetong
Published: August 2022

Many factors affect the teaching of massively open online courses (MOOCs). In this study, to explore the factors that influence the effective teaching of MOOCs, a large number of relevant studies are analyzed. Based on grounded theory, semi-structured interviews were conducted with 30 students and teachers who used MOOCs for online teaching. The interview data were subjected to four research processes –open coding, axial coding, selective coding, and saturation testing– to explore the factors influencing MOOCs’ effective teaching and the interactions between them. The results demonstrate that: (1) Effective teachers, effective tuition, effective communication, active online learning, social support guarantees, and online course design have important positive effects on effective teaching, while only certain online learning behaviors will seriously affect the teaching effectiveness of MOOC, resulting in negative effects. (2) Effective communication is essential for effective teaching in MOOCs; effective teachers are the leading factor, thus teachers should take the initiative to study and understand the students to understand their various learning needs and difficulties. (3) Reasonable and effective classroom teaching design is key to improving MOOCs’ teaching efficiency. (4) E-learning is respected, cared for, and valued by society, including cognition, emotion, and learning platform support from family, school, teachers, and classmates, and has an important impact on students’ motivation and the effects of online learning. The results of this study further clarify factors influencing effective teaching of MOOCs, thus helping to enrich and supplement the theory of effective teaching and evaluation and providing theoretical guidance for teachers to effectively implement MOOC teaching.
The psychometric properties of the Persian version of the moral injury symptoms scale-health care professionals version
Authors: Malakoutikhah Alireza , Zakeri Mohammad Ali , Koenig Harold G. , Dehghan Mahlagha
Published: August 2022

Background

Health care professionals face a number of problems during crises, such as the COVID-19. Studies addressed the prevalence of moral injury among healthcare professionals during the COVID-19 outbreak. Lack of a valid standard of moral injury among health care professionals is one of the factors that has made it difficult to identify and treat this complication. This study aimed to evaluate the psychometric properties of the Moral Injury Symptoms Scale-Health Care Professionals (MISS-HP) among health care professionals in Iran.

Methods

This study was conducted to evaluate the validity and reliability of the MISS-HP. The sample included 455 healthcare professionals working in four teaching hospitals in Kerman, who were in direct contact with patients. In this study, face validity, content validity, construct validity (structural and convergent), and internal reliability of the MISS-HP were evaluated. Demographic information questionnaire, the Moral Injury Symptoms Scale-HealthCare Professionals (MISS-HP), General Health Questionnaire (GHQ), and Impact of Event Scale (IES) were administered to study participants.

Results

The MISS-HP was evaluated using translation-back translation technique. The content validity index of the items (I-CVI) and the scale (S-CVI) were 0.9 and 0.99, respectively. Exploratory factor analysis showed a three-factor structure in the MISS-HP that explained 57.49% of the variance. Confirmatory factor analysis indices were acceptable. The cut-off point of the questionnaire was 36.5. There was a positive and moderate correlation between the Persian version of MISS-HP, GHQ (r = 0.34), and IES-R (r = 0.40). The Cronbach’s alpha coefficient of the Persian version of MISS-HP was 0.70.

Conclusion

This study found that the MISS-HP is a concise, comprehensive, valid and reliable scale for assessing moral injury among health care professionals in clinical or research settings. This scale will be helpful for managers and researchers to identify and plan health policies and improve the psychological state of health care professionals.
Burnout, psychopathology and purpose in life in healthcare workers during COVID-19 pandemic
Authors: O'Higgins Marcelo , Rojas Luz Angela , Echeverria Iván , Roselló-Jiménez Lorena , Benito Ana , Haro Gonzalo
Published: August 2022

Background

The COVID-19 pandemic has led to a significant increase in the workload of healthcare workers that, together with the risks associated with exposure to this new virus, has affected their mental health.

Objectives

The objective of the current study was to determine the prevalence of psychopathology and burnout syndrome in healthcare workers and the predictive role of purpose in life and moral courage in this relationship.

Methods

A cross-sectional study was carried out in 115 Spanish healthcare workers.

Results

Participants with burnout had higher anxiety (p = 0.001), depression (p < 0.001), post-traumatic stress (p = 0.01) and alcohol consumption (p = 0.03) levels. The different components of burnout (emotional fatigue and despersonalization) were associated with the occurrence of anxiety (OR = 0.31) and depression (OR = 0.26), respectively. A strong purpose in life decreased emotional fatigue (OR = −0.39) depersonalization (OR = −0.23) scores, increased personal accomplishment (OR = 0.52), subsequently reducing burnout levels (OR = −0.45).

Conclusions

Purpose in life was most strongly related to decreased levels of burnout. Furthermore, an association between anxiety, depression and the components of burnout was found.
Aerial Continuum Manipulation: A New Platform for Compliant Aerial Manipulation
Authors: Jalali Amir , Janabi-Sharifi Farrokh
Published: August 2022

Traditional aerial manipulation systems were usually composed of rigid-link manipulators attached to an aerial platform, arising several rigidity-related issues such as difficulties of reach, compliant motion, adaptability to object’s shape and pose uncertainties, and safety of human-manipulator interactions, especially in unstructured and confined environments. To address these issues, partially compliant manipulators, composed of rigid links and compliant/flexible joints, were proposed; however, they still suffer from insufficient dexterity and maneuverability. In this article, a new set of compliant aerial manipulators is suggested. For this purpose, the concept of aerial continuum manipulation system (ACMS) is introduced, several conceptual configurations are proposed, and the functionalities of ACMSs for different applications are discussed. Then, the performances of proposed aerial manipulators are compared with conventional aerial manipulators by implementing available benchmarks in the literature. To enhance the comparison, new features with related benchmarks are presented and used for evaluation purposes. In this study, the advantages of ACMSs over their rigid-link counterparts are illustrated and the potential applications of ACMSs are suggested. The open problems such as those related to dynamic coupling and control of ACMSs are also highlighted.
Effect of two-weeks of school-based sprint training on physical fitness, risk factors for cardiometabolic diseases and cognitive function in adolescent girls: A randomized controlled pilot trial
Authors: Williams Ryan A. , Dring Karah J. , Morris John G. , Sunderland Caroline , Nevill Mary E. , Cooper Simon B.
Published: August 2022

Background

School-based physical activity interventions are accessible to most adolescents and could enhance adolescent cardiometabolic health and cognition; yet the feasibility and success of school-based physical activity interventions is understudied.

Methods

Sixteen adolescent girls (age: 11.7 ± 0.3 y; height: 1.58 ± 0.07 m; body mass: 45.5 ± 9.2 kg) were randomized to either an intervention (2-weeks sprint training;n= 8) or control group (continuation of regular physical activity levels;n= 8). Following familiarization, all participants completed baseline measurements including fasted and postprandial capillary blood samples, a battery of cognitive function tests (Stroop Test, Sternberg Paradigm and Flanker Task), and an assessment of physical fitness (20 m sprint and multi-stage fitness test). The intervention group completed 2-weeks progressive sprint training (3 sessions per week: week one 6 × 10 s sprints, week two 8 × 10 s sprints). Follow-up measurements were completed 48 h after the final sprint training session. Data were analyzedviaANCOVA to examine between group differences at follow-up whilst controlling for baseline score.

Results

Accuracy in the intervention group during the three-item Sternberg paradigm was greater when compared with the control group (Intervention: 99.6 ± 1.1%; Control: 97.7 ± 2.2%, p = 0.046). BDNF concentration was also higher in the intervention group at follow-up than control group (Intervention: 39.12 ± 9.88 ng.ml−1; Control: 22.95 ± 9.13 ng.ml−1, p < 0.001). There were no differences at follow-up between the intervention and control group for measures of cardiometabolic health (fasted cytokine concentrations or postprandial glycaemic and insulinaemic responses) or on the Stroop Test or Flanker Task (all p > 0.05). However, the intervention group reported enjoying the sprint training and that they found the sessions valuable.

Conclusion

Two-weeks sprint interval training in a school-setting enhanced working memory and increased concentrations of BDNF in adolescent girls. The intervention was deemed enjoyable and worthwhile by the adolescent girls and thus the longer-term implementation of such an intervention should be examined.
Changes in the Fitness Fatness Index following reduced exertion high-intensity interval training versus moderate-intensity continuous training in physically inactive adults
Authors: Leahy Daniel J. , Dalleck Lance C. , Ramos Joyce S.
Published: August 2022

Background

Many adults do not reach the recommended exercise participation guidelines, often citing lack of time as a barrier. Reduced exertion high-intensity training (REHIT) is a mode of exercise that takes as few as 10 min and has been shown to be as effective as other modalities. The Fitness Fatness Index (FFI) is a recently developed index that is used to predict cardiovascular disease (CVD) risk. The aim of this study was to determine the efficacy of a REHIT vs. a traditional moderate-intensity continuous training (MICT) on FFI in physically inactive adults.

Methods

Thirty-two participants were randomized into one of two 8-week exercise intervention groups: (i) REHIT (n = 16); (ii) MICT (n = 16). The REHIT group performed 10 min of individualized cycling intervals on 2–4 days of the week. The MICT group were prescribed aerobic exercise at 50–65% of their heart rate reserve (HRR) on 3–5 days of the week. FFI was recorded at baseline and post 8-weeks, with FFI being calculated as cardiorespiratory fitness (CRF) (expressed as metabolic equivalents) divided by waist to height ratio (WtHR). A 1-unit increase in FFI was recognized as a clinically significant change in FFI.

Results

The REHIT group showed significantly greater (+1.95, ±0.63) improvements in FFI compared to those in the MICT (+0.99, ±0.47) group (between group difference, p < 0.001). Furthermore, there was a greater proportion of participants who achieved a clinically significant change in FFI in the REHIT group (12/12, 100%) than in the MICT group (8/15, 53%) (between group difference, p = 0.01).

Conclusion

This study suggests that REHIT may be a more efficacious exercise modality to increase FFI than MICT. This outcome is beneficial as the clinician can prescribe REHIT to physically inactive adults who cite lack of time as a barrier to physical activity participation and achieve significant reductions in CVD risk.
Welfare issues and potential solutions for laying hens in free range and organic production systems: A review based on literature and interviews
Authors: Bonnefous Claire , Collin Anne , Guilloteau Laurence A. , Guesdon Vanessa , Filliat Christine , Réhault-Godbert Sophie , Rodenburg T. Bas , Tuyttens Frank A. M. , Warin Laura , Steenfeldt Sanna , Baldinger Lisa , Re Martina , Ponzio Raffaella , Zuliani Anna , Venezia Pietro , Väre Minna , Parrott Patricia , Walley Keith , Niemi Jarkko K. , Leterrier Christine
Published: August 2022

In free-range and organic production systems, hens can make choices according to their needs and desires, which is in accordance with welfare definitions. Nonetheless, health and behavioral problems are also encountered in these systems. The aim of this article was to identify welfare challenges observed in these production systems in the EU and the most promising solutions to overcome these challenges. It is based on a review of published literature and research projects complemented by interviews with experts. We selected EU specific information for welfare problems, however, the selected literature regarding solutions is global. Free range use may increase the risk of infection by some bacteria, viruses and parasites. Preventive methods include avoiding contamination thanks to biosecurity measures and strengthening animals' natural defenses against these diseases which can be based on nutritional means with new diet components such as insect-derived products, probiotics and prebiotics. Phytotherapy and aromatherapy can be used as preventive and curative medicine and vaccines as alternatives to antibiotics and pesticides. Bone quality in pullets and hens prevents keel deviations and is favored by exercise in the outdoor range. Free range use also lead to higher exposure to variable weather conditions and predators, therefore shadow, fences and guard animals can be used to prevent heat stress and predation respectively. Granting a free range provides opportunities for the expression of many behaviors and yet many hens usually stay close to the house. Providing the birds with trees, shelters or attractive plants can increase range use. Small flock sizes, early experiences of enrichment and personality traits have also been found to enhance range use. Severe feather pecking can occur in free range production systems, although flocks using the outdoor area have better plumage than indoors. While many prevention strategies are facilitated in free range systems, the influence of genetics, prenatal and nutritional factors in free range hens still need to be investigated. This review provides information about practices that have been tested or still need to be explored and this information can be used by stakeholders and researchers to help them evaluate the applicability of these solutions for welfare improvement.
Metabolomics Analysis of Lettuce (Lactuca sativa L.) Affected by Low Potassium Supply
Authors: Gao Hongyan , Gong Liyan , Ni Jiheng , Li Qinglin
Published: August 2022

Lettuce is a commercially significant leafy vegetable worldwide. Potassium (K) is an essential macronutrient for lettuce growth and development and significantly impacts its metabolites. Biomarkers that are indicative of variations in the K status of lettuce before the occurrence of biophysical changes (e.g., leaf or canopy morphological, textural and color features), can be adopted to determine the early K status of lettuce. To understand the effect of low K on diverse metabolites, we examined the metabolic response of lettuce in a closed cultivation room under controlled conditions. The evaluation was based on large-scale untargeted metabolomics assay of the K group using liquid chromatography-mass spectrometry. Data were analyzed with a fold-change (FC) analysis, t-test, and orthogonal partial least square-discriminant analysis. Fifty-two metabolites were classified into two groups by the FC, p, and the variable importance in projection (VIP). Low K led to an increment in 40 metabolites (FC > 2, p < 0.05, VIP > 1) and a decrease in 12 metabolites (FC < 0.5, p < 0.05, VIP > 1). Ten pathways were significantly enriched with metabolic biomarkers. In light of the complex interactive relationships among N, P, and K, the top five biomarkers were screened further by VIP > 4.00. Adenosine, FA 18:1+3O, uridine, cis-aconitate, and D(-)-gulono-gamma-lactone showed an increase in low-K stress samples, and may be considered potential metabolic biomarkers. This study validates the impact of low K on lettuce metabolism, and identifies biomarkers that can be used to monitor the K status in lettuce.
Defining Terms Used for Animals Working in Support Roles for People with Support Needs
Authors: Howell Tiffani J. , Nieforth Leanne , Thomas-Pino Clare , Samet Lauren , Agbonika Sunday , Cuevas-Pavincich Francisca , Fry Nina Ekholm , Hill Kristine , Jegatheesan Brinda , Kakinuma Miki , MacNamara Maureen , Mattila-Rautiainen Sanna , Perry Andy , Tardif-Williams Christine Y. , Walsh Elizabeth Ann , Winkle Melissa , Yamamoto Mariko , Yerbury Rachel , Rawat Vijay , Alm Kathy , Avci Ashley , Bailey Tanya , Baker Hannah , Benton Pree , Binney Catherine , Boyle Sara , Brandes Hagit , Carr Alexa M. , Coombe Wendy , Coulter Kendra , Darby Audrey , Davies Lowri , Delisle Esther , Enders-Slegers Marie-Jose , Fournier Angela , Fox Marie , Gee Nancy , Graham Taryn M. , Hamilton-Bruce Anne , Hansen Tia G. B. , Hart Lynette , Heirs Morag , Hooper Jade , Howe Rachel , Johnson Elizabeth , Jones Melanie , Karagiannis Christos , Kieson Emily , Kim Sun-A , Kivlen Christine , Lanning Beth , Lewis Helen , Linder Deborah , Mai Dac Loc , Mariti Chiara , Mead Rebecca , Ferreira Gilly Mendes , Ngai Debbie , O’Keeffe Samantha , O’Connor Grainne , Olsen Christine , Ormerod Elizabeth , Power Emma R. , Pritchard Peggy A. , Rodriguez Kerri , Rook Deborah , Ruby Matthew B. , Schofield Leah , Signal Tania , Steel Jill , Stone Wendy , Symonds Melissa , Rooy Diane van , Warda Tiamat , Wilson Monica , Young Janette , Bennett Pauleen
Published: August 2022

The nomenclature used to describe animals working in roles supporting people can be confusing. The same term may be used to describe different roles, or two terms may mean the same thing. This confusion is evident among researchers, practitioners, and end users. Because certain animal roles are provided with legal protections and/or government-funding support in some jurisdictions, it is necessary to clearly define the existing terms to avoid confusion. The aim of this paper is to provide operationalized definitions for nine terms, which would be useful in many world regions: “assistance animal”, “companion animal”, “educational/school support animal”, “emotional support animal”, “facility animal”, “service animal”, “skilled companion animal”, “therapy animal”, and “visiting/visitation animal”. At the International Society for Anthrozoology (ISAZ) conferences in 2018 and 2020, over 100 delegates participated in workshops to define these terms, many of whom co-authored this paper. Through an iterative process, we have defined the nine terms and explained how they differ from each other. We recommend phasing out two terms (i.e., “skilled companion animal” and “service animal”) due to overlap with other terms that could potentially exacerbate confusion. The implications for several regions of the world are discussed.
Animal Detection and Classification from Camera Trap Images Using Different Mainstream Object Detection Architectures
Authors: Tan Mengyu , Chao Wentao , Cheng Jo-Ku , Zhou Mo , Ma Yiwen , Jiang Xinyi , Ge Jianping , Yu Lian , Feng Limin
Published: August 2022

Camera traps are widely used in wildlife surveys and biodiversity monitoring. Depending on its triggering mechanism, a large number of images or videos are sometimes accumulated. Some literature has proposed the application of deep learning techniques to automatically identify wildlife in camera trap imagery, which can significantly reduce manual work and speed up analysis processes. However, there are few studies validating and comparing the applicability of different models for object detection in real field monitoring scenarios. In this study, we firstly constructed a wildlife image dataset of the Northeast Tiger and Leopard National Park (NTLNP dataset). Furthermore, we evaluated the recognition performance of three currently mainstream object detection architectures and compared the performance of training models on day and night data separately versus together. In this experiment, we selected YOLOv5 series models (anchor-based one-stage), Cascade R-CNN under feature extractor HRNet32 (anchor-based two-stage), and FCOS under feature extractors ResNet50 and ResNet101 (anchor-free one-stage). The experimental results showed that performance of the object detection models of the day-night joint training is satisfying. Specifically, the average result of our models was 0.98 mAP (mean average precision) in the animal image detection and 88% accuracy in the animal video classification. One-stage YOLOv5m achieved the best recognition accuracy. With the help of AI technology, ecologists can extract information from masses of imagery potentially quickly and efficiently, saving much time.
Retrospective Evaluation of Intravenous Enoxaparin Administration in Feline Arterial Thromboembolism
Authors: Mitropoulou Athanasia , Hassdenteufel Esther , Lin Joanna , Bauer Natali , Wurtinger Gabriel , Vollmar Claudia , Henrich Estelle , Hildebrandt Nicolai , Schneider Matthias
Published: August 2022

Induction of a hypocoagulable state is imperative in the treatment of feline arterial thromboembolism. Publications in human medicine report the use of enoxaparin intravenously in selected cases. The aim of our retrospective study was to report the regain of perfusion, short-term outcome, and complications of cats treated with a novel intravenous enoxaparin protocol (1 mg/kg bolus injection followed by 3 mg/kg/day continuous infusion) combined with oral clopidogrel administration. The secondary aim was to report the monitoring of enoxaparin with anti-Xa activity. There were 36 cats included. The probability of reaching limb reperfusion was significantly (p = 0.0148) higher with anti-Xa activity within or above the target range compared to results below the target range (19/21, 90% versus 11/20, 55%). The complications observed were acute kidney injury (15/36, 42%), hemorrhage (2/36, 6%), and neurological signs (6/36, 17%). The most common causes of death/euthanasia were cardiac instability, acute kidney injury, neurological abnormalities, and limb necrosis. The hospital discharge rate was 83% (10/12) for single limb and 29% (7/24) for dual limb thrombosis; the difference was significant (p = 0.0039). The median hospitalization time for the survivors was 119.5 (95–480) h. Our study supports the use of intravenous continuous rate infusion of enoxaparin in combination with oral clopidogrel for cats with aortic thromboembolism. We report similar discharge rates and lower hemorrhage rates than previously reported with thrombolytic treatment.
Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity
Authors: Ashraf Neelma , Zafar Sana , Makitrynskyy Roman , Bechthold Andreas , Spiteller Dieter , Song Lijiang , Anwar Munir Ahmad , Luzhetskyy Andriy , Khan Ali Nisar , Akhtar Kalsoom , Khaliq Shazia
Published: August 2022

Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and biotechnology industries. In the current study, a genome-based evaluation of Streptomyces sp. isolate BR123 was analyzed to determine its biosynthetic potential, based on its in vitro antimicrobial activity against a broad range of microbial pathogens, including gram-positive and gram-negative bacteria and fungi. A draft genome sequence of 8.15 Mb of Streptomyces sp. isolate BR123 was attained, containing a GC content of 72.63% and 8103 protein coding genes. Many antimicrobial, antiparasitic, and anticancerous compounds were detected by the presence of multiple biosynthetic gene clusters, which was predicted by in silico analysis. A novel metabolite with a molecular mass of 1271.7773 in positive ion mode was detected through a high-performance liquid chromatography linked with mass spectrometry (HPLC-MS) analysis. In addition, another compound, meridamycin, was also identified through a HPLC-MS analysis. The current study reveals the biosynthetic potential of Streptomyces sp. isolate BR123, with respect to the synthesis of bioactive secondary metabolites through genomic and spectrometric analysis. Moreover, the comparative genome study compared the isolate BR123 with other Streptomyces strains, which may expand the knowledge concerning the mechanism involved in novel antimicrobial metabolite synthesis.
Outpatient Antibiotic and Antiviral Utilization Patterns in Patients Tested for Respiratory Pathogens in the United States: A Real-World Database Study
Authors: Tse Jenny , Near Aimee M. , Cheng Mindy , Karichu James , Lee Brian , Chang Susan N.
Published: August 2022

This retrospective observational study evaluated outpatient treatment patterns among patients with molecular-based viral diagnostic testing for suspected upper respiratory tract infections in the United States. Patients with a respiratory viral test were identified from 1 August 2016 to 1 July 2019 in a large national reference laboratory database linked to IQVIA’s prescription and medical claims databases. Antibiotic and influenza antiviral treatment patterns were reported up to 7 days post-test result. Predictors of antibiotic utilization were assessed using multivariable logistic regression. Among 9561 patients included in the study, 24.6% had evidence of ≥1 filled antibiotic prescription. Antibiotic utilization was higher in patients who tested negative for all viral targets (odds ratio [OR], 1.33; 95% confidence interval [CI], 1.17–1.50) and patients positive for non-influenza viruses (OR, 1.28; 95% CI, 1.09–1.51) compared with those influenza-positive only. Age ≥ 50 years and location outside of the northeast United States also predicted antibiotic utilization. Influenza antivirals were more common in influenza-positive patients compared with patients with other test results (32.5% vs. 3.6–9.0%). Thus, in this real-world study, antibiotic utilization was elevated in patients positive for non-influenza viruses, although antibiotics would generally not be indicated. Further research on pairing diagnostic tools with outpatient antibiotic stewardship programs is needed.
Evaluation of Locomotion Complexity in Zebrafish after Exposure to Twenty Antibiotics by Fractal Dimension and Entropy Analysis
Authors: Suryanto Michael Edbert , Yang Chun-Chuen , Audira Gilbert , Vasquez Ross D. , Roldan Marri Jmelou M. , Ger Tzong-Rong , Hsiao Chung-Der
Published: August 2022

Antibiotics are extensively used in aquaculture to prevent bacterial infection and the spread of diseases. Some antibiotics have a relatively longer half-life in water and may induce some adverse effects on the targeted fish species. This study analyzed the potential adverse effects of antibiotics in zebrafish at the behavioral level by a phenomic approach. We conducted three-dimensional (3D) locomotion tracking for adult zebrafish after acute exposure to twenty different antibiotics at a concentration of 100 ppb for 10 days. Their locomotor complexity was analyzed and compared by fractal dimension and permutation entropy analysis. The dimensionality reduction method was performed by combining the data gathered from behavioral endpoints alteration. Principal component and hierarchical analysis conclude that three antibiotics: amoxicillin, trimethoprim, and tylosin, displayed unique characteristics. The effects of these three antibiotics at lower concentrations (1 and 10 ppb) were observed in a follow-up study. Based on the results, these antibiotics can trigger several behavioral alterations in adult zebrafish, even in low doses. Significant changes in locomotor behavioral activity, such as total distance activity, average speed, rapid movement time, angular velocity, time in top/bottom duration, and meandering movement are highly related to neurological motor impairments, anxiety levels, and stress responses were observed. This study provides evidence based on an in vivo experiment to support the idea that the usage of some antibiotics should be carefully addressed since they can induce a significant effect of behavioral alterations in fish.
Genetic Diversity and Virulence Profiling of Multi-Drug Resistant Escherichia coli of Human, Animal, and Environmental Origins
Authors: Yar Asfand , Choudary Muhammad Adil , Rehman Abdul , Hussain Abid , Elahi Amina , ur Rehman Farooq , Waqar Ahmed Bilal , Alshammari Abdulrahman , Alharbi Metab , Nisar Muhammad Atif , Khurshid Mohsin , Khan Zaman
Published: August 2022

Rapid urbanization has increased human-animal interaction and consequently enhanced the chances to acquire zoonotic diseases. The current investigation is focused to uncover the genetic diversity of multidrug-resistant E. coli strains between different ecologies (i.e., humans, livestock, and environment) at the molecular level by employing antimicrobial resistance profiling, virulence genes profiling, and microbial typing approach using ERIC PCR. Based on multiple antibiotic resistance, overall, 19 antibiotic resistance patterns (R1–R19) were observed. Most of the strains (49/60) were detected to have the combinations of stx, eaeA, and hlyA genes and considered STEC/EPEC/EHEC. A total of 18 unique genetic profiles were identified based on ERIC-PCR fingerprints and most of the strains (13) belong to P1 whereas the least number of strains were showing profiles P7 and P8-P11 (one member each profile). The calculated values for Shannon index (H) for human, animal, and environment are 1.70, 1.82, and 1.78, respectively revealing the highest genetic diversity among the E. coli strains of animal origin. The study revealed that drug-resistant pathogenic E. coli strains could be transmitted bidirectionally among the environment, humans, and animals.
Impact of Omega-3 Fatty Acids Nano-Formulation on Growth, Antioxidant Potential, Fillet Quality, Immunity, Autophagy-Related Genes and Aeromonas hydrophila Resistance in Nile Tilapia (Oreochromis niloticus)
Authors: Ibrahim Doaa , Arisha Ahmed H. , Khater Safaa I. , Gad Wafaa M. , Hassan Zeinab , Abou-Khadra Sally H. , Mohamed Dalia Ibrahim , Ahmed Ismail Tamer , Gad Sara A. , Eid Salwa A. M. , Abd El-Wahab Reham A. , Kishawy Asmaa T. Y.
Published: August 2022

In modern aquaculture, enriching Nile tilapia’s diet with omega-3 poly-unsaturated fatty acids (PUFAs) not only plays an important role in its general health but also fortifies its fillet with omega-3-PUFAs. However, the major challenge affecting their delivery is their high instability due to oxidative deterioration. Thus, the prospective incorporation of omega-3-PUFAs into nanocarriers can enhance their stability and bioactivity. In this regard, the effect of reformulated omega-3-NPs was investigated on Nile tilapia’s performance, flesh antioxidant stability, immunity, and disease resistance. Four fish groups supplemented with omega-3-PUFAs-loaded nanoparticles (omega-3 NPs) at levels of 0, 1, 2, and 3 g/kg diet and at the end of feeding trial fish challenged with Aeromonas hydrophila. Fish performance (weight gain and feed conversion) was improved in groups supplemented with omega-3-NPs (2 and 3 g/kg diet). The deposition of omega-3-PUFAs in fish flesh elevated with increasing dietary omega-3-NPs. Simultaneously the oxidative markers (H2O2, MDA, and reactive oxygen species) in fish flesh were reduced, especially with higher omega-3-NPs. Post-challenge, downregulation of IL-1β, IL-6, IL-8, TNF-α, and caspase-1 were noticed after dietary supplementation of omega-3-NPs. Moreover, mRNA expression of autophagy-related genes was upregulated while the mTOR gene was downregulated with higher omega-3 NPs levels. Lower expression of A. hydrophila ahyI and ahyR genes were detected with omega-3 NPs supplementation. In conclusion, omega-3-NPs application can fortify tilapia flesh with omega-3-PUFAs and augment its performance, immunity, and disease resistance against Aeromonas hydrophila.