bluuebunny/crossref_metadata_2025 huggingface dataset:
The causal relationship between sarcoidosis and autoimmune diseases: a bidirectional Mendelian randomization study in FinnGen
Authors: Sun Di , Ma Ruimin , Wang Jingwei , Wang Yuanying , Ye Qiao
Published: April 2024

Background

Sarcoidosis has been considered to be associated with many autoimmune diseases (ADs), but the cause-and-effect relationship between these two diseases has not been fully explored. Therefore, the objective of this study is to explore the possible genetic association between sarcoidosis and ADs.

Methods

We conducted a bidirectional Mendelian randomization (MR) study using genetic variants associated with ADs and sarcoidosis (4,041 cases and 371,255 controls) from the FinnGen study. The ADs dataset comprised 96,150 cases and 281,127 controls, encompassing 44 distinct types of autoimmune-related diseases. Subsequently, we identified seven diseases within the ADs dataset with a case size exceeding 3,500 and performed subgroup analyses on these specific diseases.

Results

The MR evidence supported the causal association of genetic predictors of ADs with an increased risk of sarcoidosis (OR = 1.79, 95% CI = 1.59 to 2.02, P IVW-FE = 1.01 × 10-21), and no reverse causation (OR = 1.05, 95% CI 0.99 to 1.12, PIVW-MRE = 9.88 × 10-2). Furthermore, subgroup analyses indicated that genetic predictors of type 1 diabetes mellitus (T1DM), celiac disease, and inflammatory bowel disease (IBD) were causally linked to an elevated risk of sarcoidosis (All P < 6.25 × 10-3). Conversely, genetic predictors of sarcoidosis showed causal associations with a higher risk of type 1 diabetes mellitus (P < 6.25 × 10-3).

Conclusion

The present study established a positive causal relationship between genetic predictors of ADs (e.g. T1DM, celiac disease, and IBD) and the risk of sarcoidosis, with no evidence of reverse causation.
Differentially expressed proteins and microbial communities of the skin regulate disease resistance to Chinese tongue sole (Cynoglossus semilaevis)
Authors: Wang Lei , Tian Min , Chen Songlin
Published: April 2024

Vibriosis, caused by Vibrio, seriously affects the health of fish, shellfish, and shrimps, causing large economic losses. Teleosts are represent the first bony vertebrates with both innate and adaptive immune responses against pathogens. Aquatic animals encounter hydraulic pressure and more pathogens, compared to terrestrial animals. The skin is the first line of defense in fish, constituting the skin-associated lymphoid tissue (SALT), which belongs to the main mucosa-associated lymphoid tissues (MALT). However, little is known about the function of immunity related proteins in fish. Therefore, this study used iTRAQ (isobaric tags for relative and absolute quantitation) to compare the skin proteome between the resistant and susceptible families of Cynoglossus semilaevis. The protein integrin beta-2, the alpha-enolase isoform X1, subunit B of V-type proton ATPase, eukaryotic translation initiation factor 6, and ubiquitin-like protein ISG15, were highly expressed in the resistant family. The 16S sequencing of the skin tissues of the resistant and susceptible families showed significant differences in the microbial communities of the two families. The protein-microbial interaction identified ten proteins associated with skin microbes, including immunoglobulin heavy chain gene (IGH), B-cell lymphoma/leukemia 10 (BCL10) and pre-B-cell leukemia transcription factor 1 isoform X2 (PBX2). This study highlights the interaction between skin proteins and the microbial compositions of C. semilaevis and provides new insights into understanding aquaculture breeding research.
Transcriptome analysis of long non-coding RNAs in Mycobacterium avium complex–infected macrophages
Authors: Yoshida Mitsunori , Kwon Andrew Taejun , Qin Xian-Yang , Nishimura Hajime , Maeda Shiori , Miyamoto Yuji , Yoshida Yasuhiro , Hoshino Yoshihiko , Suzuki Harukazu
Published: April 2024

Mycobacterium avium complex (MAC) is a non-tuberculous mycobacterium widely distributed in the environment. Even though MAC infection is increasing in older women and immunocompromised patients, to our knowledge there has been no comprehensive analysis of the MAC-infected host-cell transcriptome—and particularly of long non-coding RNAs (lncRNAs). By using in vitro-cultured primary mouse bone-marrow-derived macrophages (BMDMs) and Cap analysis of gene expression, we analyzed the transcriptional and kinetic landscape of macrophage genes, with a focus on lncRNAs, during MAC infection. MAC infection of macrophages induced the expression of immune/inflammatory response genes and other genes similar to those involved in M1 macrophage activation, consistent with previous reports, although Nos2 (M1 activation) and Arg1 (M2 activation) had distinct expression profiles. We identified 31 upregulated and 30 downregulated lncRNA promoters corresponding respectively to 18 and 26 lncRNAs. Upregulated lncRNAs were clustered into two groups—early and late upregulated—predicted to be associated with immune activation and the immune response to infection, respectively. Furthermore, an Ingenuity Pathway Analysis revealed canonical pathways and upstream transcription regulators associated with differentially expressed lncRNAs. Several differentially expressed lncRNAs reported elsewhere underwent expressional changes upon M1 or M2 preactivation and subsequent MAC infection. Finally, we showed that expressional change of lncRNAs in MAC-infected BMDMs was mediated by toll-like receptor 2, although there may be other mechanisms that sense MAC infection. We identified differentially expressed lncRNAs in MAC-infected BMDMs, revealing diverse features that imply the distinct roles of these lncRNAs in MAC infection and macrophage polarization.
Fructose-induced metabolic reprogramming of cancer cells
Authors: Ting Kenneth K. Y.
Published: April 2024

Excess dietary fructose consumption has been long proposed as a culprit for the world-wide increase of incidence in metabolic disorders and cancer within the past decades. Understanding that cancer cells can gradually accumulate metabolic mutations in the tumor microenvironment, where glucose is often depleted, this raises the possibility that fructose can be utilized by cancer cells as an alternative source of carbon. Indeed, recent research has increasingly identified various mechanisms that show how cancer cells can metabolize fructose to support their proliferating and migrating needs. In light of this growing interest, this review will summarize the recent advances in understanding how fructose can metabolically reprogram different types of cancer cells, as well as how these metabolic adaptations can positively support cancer cells development and malignancy.
B-cell immune dysregulation with low soluble CD22 levels in refractory seronegative myasthenia gravis
Authors: Okuzono Yuumi , Miyakawa Shuuichi , Itou Tatsuo , Sagara Masaki , Iwata Masashi , Ishizuchi Kei , Sekiguchi Koji , Motegi Haruhiko , Oyama Munenori , Warude Dnyaneshwar , Kikukawa Yusuke , Suzuki Shigeaki
Published: April 2024

Myasthenia gravis (MG), primarily caused by acetylcholine receptor (AChR) autoantibodies, is a chronic autoimmune disorder causing severe muscle weakness and fatigability. In particular, seronegative MG constitutes 10%–15% of MG cases and presents diagnostic challenges especially in early-onset female patients who often show severe disease and resistance to immunosuppressive therapy. Furthermore, the immunopathology of seronegative MG remains unclear. Thus, in this study, we aimed to elucidate the pathogenic mechanism of seronegative MG using scRNA-seq analysis and plasma proteome analysis; in particular, we investigated the relationship between immune dysregulation status and disease severity in refractory seronegative MG. Employing single-cell RNA-sequencing and plasma proteome analyses, we analyzed peripheral blood samples from 30 women divided into three groups: 10 healthy controls, 10 early-onset AChR-positive MG, and 10 refractory early-onset seronegative MG patients, both before and after intravenous immunoglobulin treatment. The disease severity was evaluated using the MG-Activities of Daily Living (ADL), MG composite (MGC), and revised 15-item MG-Quality of Life (QOL) scales. We observed numerical abnormalities in multiple immune cells, particularly B cells, in patients with refractory seronegative MG, correlating with disease activity. Notably, severe MG cases had fewer regulatory T cells without functional abnormalities. Memory B cells were found to be enriched in peripheral blood cells compared with naïve B cells. Moreover, plasma proteome analysis indicated significantly lower plasma protein levels of soluble CD22, expressed in the lineage of B-cell maturation (including mature B cells and memory B cells), in refractory seronegative MG patients than in healthy donors or patients with AChR-positive MG. Soluble CD22 levels were correlated with disease severity, B-cell frequency, and RNA expression levels of CD22. In summary, this study elucidates the immunopathology of refractory seronegative MG, highlighting immune disorders centered on B cells and diminished soluble CD22 levels. These insights pave the way for novel MG treatment strategies focused on B-cell biology.
Differences in the intestinal microbiota and association of host metabolism with hair coat status in cattle
Authors: Wu Donglin , Zhao Pengfei , Wang Chunjie , Huasai Simujide , Chen Hao , Chen Aorigele
Published: April 2024

Introduction

The hair coat status of cattle serves as an easily observed indicator of economic value in livestock production; however, the underlying mechanism remains largely unknown. Therefore, the objective of the current study was to determine differences in the intestinal microbiota and metabolome of cattle based on a division of with either slick and shining (SHC) or rough and dull (MHC) hair coat in Simmental cows.

Methods

Eight SHC and eight MHC late-pregnancy Simmental cows (with similar parities, body weights, and body conditions) were selected based on their hair coat status, and blood samples (plasma) from coccygeal venipuncture and fecal samples from the rectum were collected. The intestinal microbiota (in the fecal samples) was characterized by employing 16S rRNA gene sequencing targeting the V3–V4 hypervariable region on the Illumina MiSeq PE300 platform, and plasma samples were subjected to LC–MS/MS-based metabolomics with Progenesis QI 2.3. Plasma macromolecular metabolites were examined for differences in the metabolism of lipids, proteins, mineral elements, and hormones.

Results

Notable differences between the SHC and MHC groups related to host hair coat status were observed in the host metabolome and intestinal microbiota (P < 0.05). The host metabolome was enriched in histidine metabolism, cysteine and methionine metabolism, and purine metabolism in the SHC group, and the intestinal microbiota were also enriched in histidine metabolism (P < 0.05). In the MHC group, the symbiotic relationship transitioned from cooperation to competition in the MHC group, and an uncoupling effect was present in the microbe–metabolite association of intestine microbiota–host interactions. The hubs mediating the relationships between intestinal microbiota and plasma metabolites were the intestinal bacterial genus g__norank_f__Eubacterium_coprostanoligenes_group, plasma inosine, triiodothyronine, and phosphorus, which could be used to differentiate cows’ hair coat status (P < 0.05).

Conclusion

Overall, the present study identified the relationships between the features of the intestinal microbiota and host hair coat status, thereby providing evidence and a new direction (intestine microbiota–host interplay) for future studies aimed at understanding the hair coat status of cattle.
Research progress on the inhibition of oxidative stress by teriparatide in spinal cord injury
Authors: Ai Gangtong , Xiong Moliang , Deng Liang , Zeng Jihuan , Xiao Qiang
Published: April 2024

Spinal cord injury (SCI) is currently a highly disabling disease, which poses serious harm to patients and their families. Due to the fact that primary SCI is caused by direct external force, current research on SCI mainly focuses on the treatment and prevention of secondary SCI. Oxidative stress is one of the important pathogenic mechanisms of SCI, and intervention of oxidative stress may be a potential treatment option for SCI. Teriparatide is a drug that regulates bone metabolism, and recent studies have found that it has the ability to counteract oxidative stress and is closely related to SCI. This article summarizes the main pathological mechanisms of oxidative stress in SCI, as well as the relationship between them with teriparatide, and explores the therapeutic potential of teriparatide in SCI.
Functional impact of bilateral vestibular loss and the unexplained complaint of oscillopsia
Authors: Geisinger Dario , Elyoseph Zohar , Zaltzman Roy , Mintz Matti , Gordon Carlos R.
Published: April 2024

Introduction

The vestibulo-ocular reflex (VOR) stabilizes vision during head movements. VOR disorders lead to symptoms such as imbalance, dizziness, and oscillopsia. Despite similar VOR dysfunction, patients display diverse complaints. This study analyses saccades, balance, and spatial orientation in chronic peripheral and central VOR disorders, specifically examining the impact of oscillopsia.

Methods

Participants involved 15 patients with peripheral bilateral vestibular loss (pBVL), 21 patients with clinically and genetically confirmed Machado–Joseph disease (MJD) who also have bilateral vestibular deficit, and 22 healthy controls. All pBVL and MJD participants were tested at least 9 months after the onset of symptoms and underwent a detailed clinical neuro-otological evaluation at the Dizziness and Eye Movements Clinic of the Meir Medical Center.

Results

Among the 15 patients with pBVL and 21 patients with MJD, only 5 patients with pBVL complained of chronic oscillopsia while none of the patients with MJD reported this complaint. Comparison between groups exhibited significant differences in vestibular, eye movements, balance, and spatial orientation. When comparing oscillopsia with no-oscillopsia subjects, significant differences were found in the dynamic visual acuity test, the saccade latency of eye movements, and the triangle completion test.

Discussion

Even though there is a significant VOR gain impairment in MJD with some subjects having less VOR gain than pBVL with reported oscillopsia, no individuals with MJD reported experiencing oscillopsia. This study further supports that subjects experiencing oscillopsia present a real impairment to stabilize the image on the retina, whereas those without oscillopsia may utilize saccade strategies to cope with it and may also rely on visual information for spatial orientation. Finding objective differences will help to understand the causes of the oscillopsia experience and develop coping strategies to overcome it.
Relationship between depression and sex steroid hormone among women with epilepsy
Authors: Ogunjimi Luqman , Alabi Akinyinka , Oyenuga Ibironke , Ogunkunle Jeremiah , Kasumu Emmanuel , Ogunsanya Oluwaseun , Oluseyije Oluwatobiloba , Ogunbayo Pelumi , Idume Omorojo , Kasali Adeola , Adesi Sarah , Oyebowale Mariam , Ogungbemi Damilola , Aderinola Aderonke , Irokosu Emmanuel , Murtala Abdullahi , Osalusi Bamidele
Published: April 2024

Introduction

Sex steroid hormones are emerging significant biomarkers of depression among Women with Epilepsy (WWE) with promising prognostic potential and therapeutic end point. Therefore, the study is aimed at exploring the association between sex steroids hormones, Anti-seizure Medication (ASM) and depression among WWE.

Methodology

A baseline questionnaire was used to obtain socio-demographics and clinical characteristic from one hundred and twelve (112) WWE and 50 age matched healthy control. The diagnosis of epilepsy and Electroencephalography (EEG) description was based on 2017 International League Against Epilepsy (ILAE) criteria. Blood samples were collected from cases and control during Luteal Phase (LP) and Follicular Phase (FP). The Zung Self-Rating Depression Scale (ZSRDS) was used to assess depression.

Result

The prevalence of depression among WWE is 18.8%, with a significant difference between the level of formal education (p0.000), age (p0.000), and mean ZSRDS (p0.000) among cases and control. There is a statistical difference in hormonal levels between cases and control with regards to higher testosterone [3.28 ± 9.99 vs. 0.31 ± 0.30; p0.037], lower FP prolactin [16.37 ± 20.14 vs. 17.20 ± 7.44; p0.778], and lower LP prolactin [15.74 ± 18.22 vs. 17.67 ± 7.27; p0.473]. Testosterone (p0.024), FP Follicle Stimulating Hormone (FSH) (p0.009), FP Estradiol (p0.006), LP FSH (p0.031), LP Progesterone (p0.023), and LP Prolactin (p0.000) were associated with depression. However, only prolactin (p0.042) and testosterone (p0.000) predicts depression among WWE.

Conclusion

There was higher mean depression score, lower prolactin and higher testosterone level among cases compared to control. Furthermore, there was lower prolactin and higher testosterone level in Carbamazepine (CBZ) group compared to Levetiracetam (LEV) groups.
The FLASH effect—an evaluation of preclinical studies of ultra-high dose rate radiotherapy
Authors: McGarrigle Josie May , Long Kenneth Richard , Prezado Yolanda
Published: April 2024

FLASH radiotherapy (FLASH-RT) is a novel radiotherapy approach based on the use of ultra-high dose radiation to treat malignant cells. Although tumours can be reduced or eradicated using radiotherapy, toxicities induced by radiation can compromise healthy tissues. The FLASH effect is the observation that treatment delivered at an ultra-high dose rate is able to reduce adverse toxicities present at conventional dose rates. While this novel technique may provide a turning point for clinical practice, the exact mechanisms underlying the causes or influences of the FLASH effect are not fully understood. The study presented here uses data collected from 41 experimental investigations (published before March 2024) of the FLASH effect. Searchable databases were constructed to contain the outcomes of the various experiments in addition to values of beam parameters that may have a bearing on the FLASH effect. An in-depth review of the impact of the key beam parameters on the results of the experiments was carried out. Correlations between parameter values and experimental outcomes were studied. Pulse Dose Rate had positive correlations with almost all end points, suggesting viability of FLASH-RT as a new modality of radiotherapy. The collective results of this systematic review study suggest that beam parameter qualities from both FLASH and conventional radiotherapy can be valuable for tissue sparing and effective tumour treatment.
Myelopreservation with Trilaciclib in recurrent advanced ovarian cancer: a case report
Authors: Tan Huaming , Han Xiuchen , Li Chao , Liu Wenli , Li Kanghong , Sheng Xiugui , Qi Shuying
Published: April 2024

Ovarian cancer is a prevalent malignant tumor of the female reproductive system, often remaining concealed until it reaches an advanced stage. The standard treatment protocol includes cytoreductive surgery for ovarian cancer plus postoperative consolidation chemotherapy and maintenance therapy, although it carries a high recurrence rate. During the treatment period, chemotherapy can lead to bone marrow suppression, a condition known as Chemotherapy-Induced Myelosuppression (CIM). This suppression may necessitate dose reduction or chemotherapy treatment cycle delay. In severe cases, CIM can result in infection, fever, and potential harm to the patient’s life. Here, we report a case of a female patient with ovarian malignant tumor of biochemical recurrence who treated with chemotherapy combined with Trilaciclib, following previous perioperative chemotherapy with occurrence of severe CIM. It involves an intravenous injection of Trilaciclib before chemotherapy, which significantly abates the side effects of chemotherapy, reduces the occurrence of severe CIM, improves the patients’ quality of life, and decreases the economic burden of hospitalization. We hope that this retrospective analysis of the case may serve as a reference in preventing and treating severe CIM during chemotherapy in some patients with malignant tumors, ultimately benefiting more patients with tumors.
Ectopic thyroid in the hepatoduodenal ligament: a case report and literature review
Authors: Zhang Lei , Cui Xijun , Wang Baolei , Du Xiulan , Hou Guoqi , Yu Xiaoqian
Published: April 2024

Ectopic thyroid arises from abnormal development of thyroid primordial tissues as it migrates to the lower interstitium during the embryonic period, which can occur at various locations during the descent process. However, ectopic thyroid in the subdiaphragmatic area is extremely rare. In this case, we report a case of ectopic thyroid located in the hepatoduodenal ligament. The 60-year-old female patient was admitted to hospital with gallbladder stones and cholecystitis. Preoperative imaging showed a mass in the hepatoduodenal ligament. As the patient declined a needle biopsy of the mass, the nature of the mass remained unclear prior to surgery. The patient subsequently underwent laparoscopic cholecystectomy and exploratory resection of the mass. The histopathology of the resected mass showed the characteristics of ectopic thyroid, and immunohistochemical staining revealed positive expression of thyroid transcription factor-1 and thyroglobulin. The diagnosis of ectopic thyroid was established. Upon confirming the diagnosis, comprehensive neck examination revealed the presence of a normally functioning thyroid gland. Throughout the four-year follow-up period, the patient’s thyroid ultrasonography and thyroid function tests indicated no abnormalities. Ectopic thyroid in the hepatoduodenal ligament and surrounding areas is an extremely rare clinical abnormality, achieving a clear diagnosis before initiating treatment offers diagnostic and treatment insights and clues for clinicians when differentiating masses within this region.
Targeting ferroptosis as a potential strategy to overcome the resistance of cisplatin in oral squamous cell carcinoma
Authors: Chen Rongkun , Zhu Shuyu , Zhao Ruoyu , Liu Wang , Jin Luxin , Ren Xiaobin , He Hongbing
Published: April 2024

Oral squamous cell carcinoma (OSCC) is a crucial public health problem, accounting for approximately 2% of all cancers globally and 90% of oral malignancies over the world. Unfortunately, despite the achievements in surgery, radiotherapy, and chemotherapy techniques over the past decades, OSCC patients still low 5-year survival rate. Cisplatin, a platinum-containing drug, serves as one of the first-line chemotherapeutic agents of OSCC. However, the resistance to cisplatin significantly limits the clinical practice and is a crucial factor in tumor recurrence and metastasis after conventional treatments. Ferroptosis is an iron-based form of cell death, which is initiated by the intracellular accumulation of lipid peroxidation and reactive oxygen species (ROS). Interestingly, cisplatin-resistant OSCC cells exhibit lower level of ROS and lipid peroxidation compared to sensitive cells. The reduced ferroptosis in cisplatin resistance cells indicates the potential relationship between cisplatin resistance and ferroptosis, which is proved by recent studies showing that in colorectal cancer cells. However, the modulation pathway of ferroptosis reversing cisplatin resistance in OSCC cells still remains unclear. This article aims to concisely summarize the molecular mechanisms and evaluate the relationship between ferroptosis and cisplatin resistance OSCC cells, thereby providing novel strategies for overcoming cisplatin resistance and developing new therapeutic approaches.
Do we have (in)compatibilist intuitions? Surveying experimental research
Authors: Inarimori Kiichi , Honma Souichiro , Miyazono Kengo
Published: April 2024

This article critically examines the experimental philosophy of free will, particularly the interplay between ordinary individuals’ compatibilist and incompatibilist intuitions. It explores key insights from research studies that propose “natural compatibilism” and “natural incompatibilism”. These studies reveal a complex landscape of folk intuitions, where participants appear to exhibit both types of intuitions. Here, we examine error theories, which purport to explain the coexistence of apparently contradictory intuitions: the Affective Performance Error hypothesis, the “Free Will No Matter What” hypothesis, the Bypassing hypothesis, and the Intrusion hypothesis, and the article explores the cognitive errors that could shape individuals’ inconsistent perceptions of free will. We then explore three possibilities regarding folk intuitions: most individuals may hold either compatibilist or incompatibilist intuitions, both simultaneously, or neither. Our aim is to deepen the understanding of the complex dynamics of intuitions about free will, and we close with suggestions for future studies in experimental philosophy.
A call to action: informing research and practice in suicide prevention among individuals with psychosis
Authors: Chalker Samantha A. , Sicotte Roxanne , Bornheimer Lindsay A. , Parrish Emma M. , Wastler Heather , Ehret Blaire , DeVylder Jordan , Depp Colin A.
Published: April 2024

Although it is well established that individuals living with psychosis are at increased risk for suicidal ideation, attempts, and death by suicide, several gaps in the literature need to be addressed to advance research and improve clinical practice. This Call-to-Action highlights three major gaps in our understanding of the intersection of psychosis and suicide as determined by expert consensus. The three gaps include research methods, suicide risk screening and assessment tools used with persons with psychosis, and psychosocial interventions and therapies. Specific action steps to address these gaps are outlined to inform research and practice, and thus, improve care and prognoses among persons with psychosis at risk for suicide.
The influence of digital platform on the implementation of corporate social responsibility: from the perspective of environmental science development to explore its potential role in public health
Authors: Wang Mansi , Yuan Renmiao , Guan Xin , Wang Zeyu , Zeng Yanzhao , Liu Tao
Published: April 2024

Introduction

This paper aims to explore the intersection of corporate social responsibility (CSR) and public health within the context of digital platforms. Specifically, the paper explores the impact of digital platforms on the sustainable development practices of enterprises, seeking to comprehend how these platforms influence the implementation of environmental protection policies, resource management, and social responsibility initiatives.

Methods

To assess the impact of digital platforms on corporate environmental behavior, we conducted a questionnaire survey targeting employees in private enterprises. This survey aimed to evaluate the relationship between the adoption of digital platforms and the implementation of environmental protection policies and practices.

Results

Analysis of the survey responses revealed a significant positive correlation between the use of digital platforms and the environmental protection behavior of enterprises (r=0.523;p<0.001), Moreover, the presence of innovative environmental protection technologies on these platforms was found to positively influence the enforcement of environmental policies, with a calculated impact ratio of (a∗b/c=55.31%). An intermediary analysis highlighted that environmental innovation technology plays a mediating role in this process. Additionally, adjustment analysis showed that enterprises of various sizes and industries respond differently to digital platforms, indicating the need for tailored environmental policies

Discussion

These findings underscore the pivotal role of digital platforms in enhancing CSR efforts and public health by fostering improved environmental practices among corporations. The mediating effect of environmental innovation technologies suggests that digital platforms not only facilitate direct environmental actions but also enhance the efficiency and effectiveness of such initiatives through technological advances. The variability in response by different enterprises points to the importance of customizable strategies in policy formulation. By offering empirical evidence of digital platforms’ potential to advance CSR and public health through environmental initiatives, this paper contributes to the ongoing dialogue on sustainable development goals. It provides practical insights for enterprises and policy implications for governments striving to craft more effective environmental policies and strategies.
In-classroom physical activity breaks program among school children in Sri Lanka: study protocol for a randomized controlled trial
Authors: Peiris D. L. I. H. K. , Duan Yanping , Vandelanotte Corneel , Liang Wei , Baker Julien Steven
Published: April 2024

Background

The problem of sedentary behavior among primary school children is alarming, with numbers gradually increasing worldwide, including Sri Lanka. Physical activity interventions within classroom settings have been acknowledged as a critical strategy to increase students’ movement behaviors while enhancing their academic achievement and health. Yet, the busy curriculum and challenging educational demands encourage more sedentary classroom behavior. Hence, this study aims to develop and evaluate an in-classroom physical activity breaks (IcPAB) intervention among fifth graders in Sri Lanka.

Methods

The study will adopt a randomized controlled trial (RCT), comprising an in-classroom physical activity breaks program group and a control group to evaluate the effects of IcPAB on academic achievement, movement behaviors and health outcomes. The intervention design is based on the capability (C), opportunity (O) and motivation (M) behavior (B) (COM-B) model. A least 198 fifth graders will be recruited from two schools in Uva province, Sri Lanka. The recruitment process will start in late 2022. Class teachers of the intervention group will implement 5-min activity breaks at least three times a day after completing a training session. The primary variables include mathematics and reading achievement. The secondary variables include physical activity levels, steps count, sedentary behavior, body mass index, aerobic fitness, and perceived stress. Data collection will be implemented at pre-test and post-test, respectively. Intervention fidelity and the process will also be evaluated.

Discussion

The IcPAB is designed to prevent pure educational time loss by introducing curriculum-integrated short bouts of physical active breaks into the classroom routine. If the IcPAB is effective, it can (1) improve the mathematics and reading achievement of fifth-grade girls and boys, which is a significant factor determining the performance at the Grade Five National Scholarship Examination in Sri Lanka; (2) improve movement behaviors as well as physical and mental health outcomes among primary school students. Sequentially, the IcPAB will enrich school-based physical activity intervention approaches which can in turn bring academic and health benefits to primary school children in Sri Lanka.

Trial registration

The first version of the trial was registered with the ISRCTN registry (Ref: ISRCTN52180050) on 20/07/2022.
Soft bioreactor systems: a necessary step toward engineered MSK soft tissue?
Authors: Dvorak Nicole , Liu Zekun , Mouthuy Pierre-Alexis
Published: April 2024

A key objective of tissue engineering (TE) is to produce in vitro funcional grafts that can replace damaged tissues or organs in patients. TE uses bioreactors, which are controlled environments, allowing the application of physical and biochemical cues to relevant cells growing in biomaterials. For soft musculoskeletal (MSK) tissues such as tendons, ligaments and cartilage, it is now well established that applied mechanical stresses can be incorporated into those bioreactor systems to support tissue growth and maturation via activation of mechanotransduction pathways. However, mechanical stresses applied in the laboratory are often oversimplified compared to those found physiologically and may be a factor in the slow progression of engineered MSK grafts towards the clinic. In recent years, an increasing number of studies have focused on the application of complex loading conditions, applying stresses of different types and direction on tissue constructs, in order to better mimic the cellular environment experienced in vivo. Such studies have highlighted the need to improve upon traditional rigid bioreactors, which are often limited to uniaxial loading, to apply physiologically relevant multiaxial stresses and elucidate their influence on tissue maturation. To address this need, soft bioreactors have emerged. They employ one or more soft components, such as flexible soft chambers that can twist and bend with actuation, soft compliant actuators that can bend with the construct, and soft sensors which record measurements in situ. This review examines types of traditional rigid bioreactors and their shortcomings, and highlights recent advances of soft bioreactors in MSK TE. Challenges and future applications of such systems are discussed, drawing attention to the exciting prospect of these platforms and their ability to aid development of functional soft tissue engineered grafts.
Abusive use of anabolic androgenic steroids, male sexual dysfunction and infertility: an updated review
Authors: Azevedo Rafael de Almeida , Gualano Bruno , Teixeira Thiago Afonso , Nascimento Bruno C. G. , Hallak Jorge
Published: April 2024

The evolving prevalence of anabolic androgenic steroids (AAS) abuse among nonathletes is alarming because of the known harm to an individual’s health. Among the adverse effects of AAS abuse, male infertility and sexual dysfunction have been often reported in the literature, but little is known regarding its actual prevalence, possible underpinning mechanisms, and potential treatments either during or post-AAS usage. Thus, the current narrative review summarizes the state-of-art regarding the effects of AAS on male fertility and sexual function. Evidence was gathered from the latest reviews and recent original studies, specifically from prospective cohorts and clinical trials, ultimately resulting in five main topics of discussion. First, AAS usage is briefly characterized by its historical background, main physiological mechanisms, and the most frequently used AAS substances. Second, data on the prevalence of AAS-induced male infertility and sexual dysfunction are described. Third, some new insights on possible underpinning mechanisms of AAS-induced male infertility and sexual dysfunction are thoroughly discussed, with particular attention to histological data derived from animal models and the latest insights from prospective cohorts in humans. Fourth, the potential treatments during and after the AAS usage are presented, highlighting the odds of resolving male infertility and sexual dysfunction. Fifth, future directions on this topic are discussed, focusing on the methodological robustness of scientific studies.
Carbonate buffer mixture and fecal microbiota transplantation hold promising therapeutic effects on oligofructose-induced diarrhea in horses
Authors: Tuniyazi Maimaiti , Tang Ruibo , Hu Xiaoyu , Fu Yunhe , Zhang Naisheng
Published: April 2024

Diarrhea is a common gastrointestinal disorder in horses, with diet-induced diarrhea being an emerging challenge. This study aimed to investigate the gut microbiota differences in healthy and diet-induced diarrheic horses and evaluate the effectiveness of fecal microbiota transplantation (FMT) and carbonate buffer mixture (CBM) as potential therapeutic approaches. Twenty healthy horses were included in the study, with four groups: Control, Diarrhea, CBM, and FMT. Diarrhea was induced using oligofructose, and fecal samples were collected for microbiota analysis. FMT and CBM treatments were administered orally using donor fecal matter, and formula mixture, respectively. Clinical parameters, serum levels, intestinal tissue histopathology, and fecal microbiota profiles were evaluated. The results showed that diarrhea induction disbalanced the gut microbiota with decreased diversity and richness, affected clinical parameters including elevated body temperature and diarrhea score, and decreased fecal pH, increased inflammatory responses such as increased serum LPS, IL-17A, lactic acid and total protein, and caused damage in the colon tissue. CBM and FMT treatments altered the gut microbiota composition, restoring it towards a healthier profile compared to diarrheic, restored the gut microbiota composition to healthier states, improved clinical symptoms including decreased body temperature and diarrhea score, and increased fecal pH, decreased inflammatory responses such as increased serum LPS, IL-17A, lactic acid and total protein, and repaired tissue damage. CBM and FMT Spearman correlation analysis identified specific bacterial taxa associated with host parameters and inflammation. FMT and CBM treatments showed promising therapeutic effects in managing oligofructose-induced diarrhea in horses. The findings provide valuable insights into the management and treatment of diarrhea in horses and suggest the potential of combined CBM and FMT approaches for optimal therapeutic outcomes.
Applied models and molecular characteristics of small cell lung cancer
Authors: Fűr Gabriella Mihalekné , Nemes Kolos , Magó Éva , Benő Alexandra Á. , Topolcsányi Petronella , Moldvay Judit , Pongor Lőrinc S.
Published: April 2024

Small cell lung cancer (SCLC) is a highly aggressive type of cancer frequently diagnosed with metastatic spread, rendering it surgically unresectable for the majority of patients. Although initial responses to platinum-based therapies are often observed, SCLC invariably relapses within months, frequently developing drug-resistance ultimately contributing to short overall survival rates. Recently, SCLC research aimed to elucidate the dynamic changes in the genetic and epigenetic landscape. These have revealed distinct subtypes of SCLC, each characterized by unique molecular signatures. The recent understanding of the molecular heterogeneity of SCLC has opened up potential avenues for precision medicine, enabling the development of targeted therapeutic strategies. In this review, we delve into the applied models and computational approaches that have been instrumental in the identification of promising drug candidates. We also explore the emerging molecular diagnostic tools that hold the potential to transform clinical practice and patient care.
Research on a Fast Image-Matching Algorithm Based on Nonlinear Filtering
Authors: Yin Chenglong , Zhang Fei , Hao Bin , Fu Zijian , Pang Xiaoyu
Published: April 2024

Computer vision technology is being applied at an unprecedented speed in various fields such as 3D scene reconstruction, object detection and recognition, video content tracking, pose estimation, and motion estimation. To address the issues of low accuracy and high time complexity in traditional image feature point matching, a fast image-matching algorithm based on nonlinear filtering is proposed. By applying nonlinear diffusion filtering to scene images, details and edge information can be effectively extracted. The feature descriptors of the feature points are transformed into binary form, occupying less storage space and thus reducing matching time. The adaptive RANSAC algorithm is utilized to eliminate mismatched feature points, thereby improving matching accuracy. Our experimental results on the Mikolajcyzk image dataset comparing the SIFT algorithm with SURF-, BRISK-, and ORB-improved algorithms based on the SIFT algorithm conclude that the fast image-matching algorithm based on nonlinear filtering reduces matching time by three-quarters, with an overall average accuracy of over 7% higher than other algorithms. These experiments demonstrate that the fast image-matching algorithm based on nonlinear filtering has better robustness and real-time performance.
Predicting the Aggregate Mobility of a Vehicle Fleet within a City Graph
Authors: Sánchez-Rada J. Fernando , Vila-Rodríguez Raquel , Montes Jesús , Zufiria Pedro J.
Published: April 2024

Predicting vehicle mobility is crucial in domains such as ride-hailing, where the balance between offer and demand is paramount. Since city road networks can be easily represented as graphs, recent works have exploited graph neural networks (GNNs) to produce more accurate predictions on real traffic data. However, a better understanding of the characteristics and limitations of this approach is needed. In this work, we compare several GNN aggregated mobility prediction schemes to a selection of other approaches in a very restricted and controlled simulation scenario. The city graph employed represents roads as directed edges and road intersections as nodes. Individual vehicle mobility is modeled as transitions between nodes in the graph. A time series of aggregated mobility is computed by counting vehicles in each node at any given time. Three main approaches are employed to construct the aggregated mobility predictors. First, the behavior of the moving individuals is assumed to follow a Markov chain (MC) model whose transition matrix is inferred via a least squares estimation procedure; the recurrent application of this MC provides the aggregated mobility prediction values. Second, a multilayer perceptron (MLP) is trained so that—given the node occupation at a given time—it can recursively provide predictions for the next values of the time series. Third, we train a GNN (according to the city graph) with the time series data via a supervised learning formulation that computes—through an embedding construction for each node in the graph—the aggregated mobility predictions. Some mobility patterns are simulated in the city to generate different time series for testing purposes. The proposed schemes are comparatively assessed compared to different baseline prediction procedures. The comparison illustrates several limitations of the GNN approaches in the selected scenario and uncovers future lines of investigation.
Pediatric Ischemic Stroke: Clinical and Paraclinical Manifestations—Algorithms for Diagnosis and Treatment
Authors: Wessel Niels , Sprincean Mariana , Sidorenko Ludmila , Revenco Ninel , Hadjiu Svetlana
Published: April 2024

Childhood stroke can lead to lifelong disability. Developing algorithms for timely recognition of clinical and paraclinical signs is crucial to ensure prompt stroke diagnosis and minimize decision-making time. This study aimed to characterize clinical and paraclinical symptoms of childhood and neonatal stroke as relevant diagnostic criteria encountered in clinical practice, in order to develop algorithms for prompt stroke diagnosis. The analysis included data from 402 pediatric case histories from 2010 to 2016 and 108 prospective stroke cases from 2017 to 2020. Stroke cases were predominantly diagnosed in newborns, with 362 (71%, 95% CI 68.99–73.01) cases occurring within the first 28 days of birth, and 148 (29%, 95% CI 26.99–31.01) cases occurring after 28 days. The findings of the study enable the development of algorithms for timely stroke recognition, facilitating the selection of optimal treatment options for newborns and children of various age groups. Logistic regression serves as the basis for deriving these algorithms, aiming to initiate early treatment and reduce lifelong morbidity and mortality in children. The study outcomes include the formulation of algorithms for timely recognition of newborn stroke, with plans to adopt these algorithms and train a fuzzy classifier-based diagnostic model using machine learning techniques for efficient stroke recognition.
Performance of Pile–Wall System Adjacent to Footings
Authors: Sudani Ghassan A. , Jao Mien
Published: April 2024

The performance of a retaining wall is dependent on multiple factors including lateral earth pressure, which results from backfill soils and adjacent footings located behind a retaining wall. The prediction of a retaining wall’s performance in a footing–soil–wall system (FSPS) must incorporate the influences caused by the movement of a retaining wall. This study examines the performance of a retaining wall formed by driven, precast, concrete piles located adjacent to a concrete footing using two- and three-dimensional finite element analysis (2D and 3D FEA) by ANSYS 13.0 software. Both soil and concrete are assumed to behave as non-linear, elastic-perfectly plastic and rate-independent materials in compliance with the upper-bound model of Drucker–Prager yield criterion. Three backfill and foundation soils are considered: kaolin, silty clay, and kaolin–sand. Various conditions of soil type, footing shape ratio, pile width, and footing–pile distance through 180 FEA runs are investigated. The effects of 2D and 3D FEA on the behavior of the pile–wall system are compared. The lateral deflection and pressure distribution profiles along the pile–wall are studied and presented. Two empirical equations predicting lateral deflections at the pile toe and pile head and useful for pile structural design are developed under the ultimate pressure of the adjacent footing.
A TEE-Based Federated Privacy Protection Method: Proposal and Implementation
Authors: Zhang Libo , Duan Bing , Li Jinlong , Ma Zhan’gang , Cao Xixin
Published: April 2024

With the continuous enhancement of privacy protection globally, there is a problem for the traditional machine learning paradigm, which is that training data cannot be obtained from a single place. Federated learning is considered a viable technique for preserving privacy that can train deep models with decentralized data. Aiming at two-party vertical federated learning, and at common attack problems such as model inversion, gradient leakage, and data theft, we provide a formal definition of Intel SGX’s trusted computing base, remote attestation, integrity verification, and encrypted storage, and propose a general federated learning privacy enhancement algorithm in the scenario of a malicious adversary model, and we extend this method to support horizontal federated learning, secure outsourced computation, etc. Furthermore, the method is developed in a Fedlearner framework of open-sourced machine learning to achieve privacy protection of the training data and model without any modification to the existing neural network and algorithm running on the framework. The experimental results show that this scheme substantially improves on the existing schemes in terms of training efficiency, without losing model accuracy.
Optimal Variables for Retrieval Products
Authors: Ceccherini Simone
Published: April 2024

The increase in satellite instruments sounding the atmosphere will increase the frequency of several instruments simultaneously measuring either the same vertical profile or vertical profiles related to nearby geo-locations, and users will consult fused products rather than individual measurements. Therefore, the retrieval products should be optimized for use in data fusion operations, rather than for the representation of the profile. This change in paradigm raises the question of whether a more functional representation of the retrieval products exists. New variables for the retrieval products are proposed that have several advantages with respect to the standard retrieval products. These variables, in the linear approximation of the forward model, are independent of the a priori information used in the retrieval, allow us to represent the profile with any a priori information and can be used directly to perform the data fusion of a set of measurements. Furthermore, the use of these variables allows us to reduce the stored data to about one third of its volume with respect to the use of standard retrieval products.