bluuebunny/crossref_metadata_2025 huggingface dataset:
“Meus filhos nenhum sabe falar o alemão [...] Mas hoje é tarde, não posso fazer ensinar”
Authors: Souza Pedro Daniel dos Santos , Barbosa Davi Souza Pereira
Published: March 2024

A imigração dos povos germânicos para o Brasil remonta ao período imperial e, durante a II Guerra Mundial, essa comunidade sofreu com as medidas nacionalistas implementadas pelo governo varguista. A par disso, este trabalho pretende refletir sobre as formas de silenciamento e de repressão sofridas pela língua alemã no âmbito da execução da campanha de nacionalização da Era Vargas. O presente estudo está ancorado nos aportes teóricos do campo da História Social Linguística do Brasil (Mattos e Silva, 1998). A discussão focaliza o Decreto-Lei n.º 406/1938 e a política de repressão às línguas de imigração, cotejando com as narrativas registradas no documentário Proibido falar alemão, sobretudo quanto ao impacto da intervenção do Estado e suas consequências. Os resultados desse estudo apontam que o governo varguista, fazendo uso do nacionalismo, populismo e autoritarismo vigentes na década de 1930, promoveu uma política de repressão linguística, caracterizada pela proibição de uso das línguas de imigração e, em particular, da língua alemã. Entre as medidas tomadas pelo Governo, estavam a proibição do falar alemão em locais públicos e privados, apreensão dos livros didáticos e interferência nas práticas pedagógicas nas escolas alemãs, controle sobre a produção jornalística, além da grande perseguição em diversos outros contextos sociais.
Multimodalidade e aquisição de L2
Authors: Frye Simone , Moraes Antônio Henrique Coutelo de , Fonte Renata Fonseca Lima de
Published: March 2024

Este artigo versa sobre os gestos na aquisição do inglês como segunda língua (L2) em contexto interativo entre mãe e criança bilíngues durante a realização de uma atividade escolar na pandemia da COVID-19. Propõe-se analisar a produção gestual no uso da língua inglesa por uma criança durante a realização de atividade escolar em contexto da pandemia da COVID-19, sob a mentoria de sua mãe e os impactos deste contexto na aquisição e desenvolvimento do inglês como L2. A pesquisa fundamenta-se na perspectiva multimodal, na qual gesto e produção vocal integram a mesma matriz linguístico-cognitiva e nos estudos sobre bilinguismo. A metodologia de abordagem qualitativa transversal compreendeu um estudo de caso, do qual participou uma criança bilíngue de 5 anos e sua mãe, mediadora das atividades escolares, videografadas, na pandemia da COVID 19. Os resultados indicaram a produção de gestos dêiticos, icônicos e emblemáticos, que tiveram papéis de organizadores linguísticos. Os achados evidenciaram o funcionamento multimodal da linguagem na aquisição de L2 em cenário pandêmico. A mãe teve seu papel ressignificado ao reinventar-se como mentora da filha na mediação da relação escola bilíngue-aluna, durante realização de atividade escolar no período da pandemia da COVID-19.
Peer-Reviews and AI Feedback Compared: University Students’ Preferences
Authors: Zapata Gabriela C. , Saini Akash K. , Cope Bill , Kalantzis Mary , Searsmith2 Duane
Published: March 2024

This article seeks to contribute to the existing literature on peer review by comparing human with machine reviews based on the implementation of a chatbot responding with reference to a large language model for the review of open-ended student work. Specifically, we sought to investigate how 91 graduate students in a public American university regarded both types of feedback after experiencing them on the same works. Data consisted of the participants’ written reflections comparing both review types after being exposed to them at least once, as well as the words they submitted to convey their views of peer comments. The thematic analysis of the data revealed a preference for human reviews, with students’ identifying a variety of academic and personal benefits. Despite this partiality, the reflections submitted by all participants also pointed to their acceptance of machine feedback as a welcomed complement to peer reviews, stating that the combination of both types of reviews had enriched their academic experience.
Pengaruh Shadow Dan Shuttle Run Terhadap Footwork Bulutangkis PB Jaya Raya Metland
Authors: Denatara Eskar Tri , Wibowo Febri Prasetyo Ari , Wahyu Adam Putra
Published: March 2024

Penelitian ini bertujuan untuk mengetahui pengaruh latihan shadow dan shuttle run terhadap footwork olahraga bulutangkis usia 11 - 13 tahun PB Jaya Raya Metland. Penelitian ini menggunakan metode eksperimen dengan desain “two groups pre-test-post-test design”. Populasi dalam penelitian ini adalah atlet PB Jaya Raya Metland yang berjumlah 30 orang. Teknik sampling menggunakan purposive sampling, dengan kriteria yaitu: (1) daftar hadir latihan dua bulan terakhir minimal 75% (keaktifan mengikuti latihan), (2) pemain merupakan atlet PB. Jaya Raya Jakarta, (3) berusia 13-15 tahun, (4) berjenis kelamin laki-laki. Berdasarkan kriteria tersebut yang memenuhi berjumlah 20 atlet. Analisis data menggunakan uji t. Berdasarkan hasil penelitian dapat disimpulkan bahwa: (1) Ada pengaruh latihan shadow terhadap footwork olahraga bulutangkis usia 11-13 tahun PB Jaya Raya Mentland, dengan thitung 3,289 > ttabel 2,26 dan nilai signifikansi 0,009 < 0,05 dengan pengaruh sebesar 9,55%. (2) Ada pengaruh latihan shuttle run terhadap footwork olahraga bulutangkis usia 11-13 tahun PB Jaya Raya Mentland, dengan thitung 4,155 > ttabel 2,26 dan nilai signifikansi 0,002 < 0,05 dengan pengaruh sebesar 10,69%. (3) Metode shuttle run lebih efektif untuk footwork olahraga bulutangkis usia 11-13 tahun PB Jaya Raya Mentland daripada metode shadow dengan selisih rata-rata post-test sebesar 0,2619.
Lompat Jauh Gaya Jongkok
Authors: Ishak Muhammad , Hakim Hikmad , Sahabuddin Sahabuddin , Bismar Ahmad Rum
Published: March 2024

Penelitian ini bertujuan untuk mengetahui hubungan panjang tungkai dan kecepatan lari terhadap kemampuan lompat jauh gaya jongkok. Penelitian ini termasuk jenis penelitian deskriptif. Populasi penelitian ini adalah seluruh murid SD Inpres Perumnas Kota Makassar dengan jumlah sampel penelitian 40 orang siswa putra yang dipilih secara random sampling. Teknik analisis data yang digunakan adalah teknik analisis korelasi dan regresi dengan menggunakan sistem SPSS Versi 22.00 pada taraf signifikan 95% atau a0,05.Bertolak dari hasil  analisis data, maka penelitian ini menyimpulkan bahwa: (1) Ada hubungan yang signifikan panjang tungkai terhadap kemampuan lompat jauh gaya jongkok, terbukti nilai ro = -0,845 (P < a = 0,05); (2) Ada hubungan yang signifikan kecepatan lari terhadap kemampuan lompat jauh gaya jongkok, terbukti nilai ro = 0,771 (P < a = 0,05); dan, (3) Ada hubungan yang signifikan antara panjang tungkai dan kecepatan lari terhadap kemampuan lompat jauh gaya jongkok, terbukti nilai Ro = 0,847 (P < a = 0,05).
Pengaruh Jarak Antara Rumah Ke Sekolah dan Minat Belajar PJOK Siswa di SMP Negeri Se- Kecamatan Menteng
Authors: Aisyah Audi Putri , Rihatno Taufik , Nurulfa Rizky
Published: March 2024

Tujuan dari penelitian ini adalah untuk mengetahui ada tidaknya pengaruh dari jarak tempat tinggal terhadap hasil belajar PJOK siswa di SMP Negeri Se- Kecamatan Menteng. Mengetahui pengaruh pengaruh jarak tempat tinggal terhadap hasil belajar PJOK siswa di SMP Negeri Se- Kecamatan Menteng tahun ajaran 2022/2023. Desain dalam penelitian ini menggunakan metode deskriptif dengan menggunakan pendekatan kuantitatif yakni mendeskripsikan mengenai pengaruh Jarak tempuh siswa ke sekolah terhadap prestasi belajar siswa di Sekolah. Metode yang digunakan dalam penelitian ini adalah metode survei, sedangkan teknik pengumpulan datanya dengan menggunakan angket, skor yang diperoleh dari angket kemudian dianalisis menggunakan analisis deskriptif kuantitatif yang dituangkan dalam bentuk prosentase. Dari data diatas menjelaskan bahwa siswa yang memiliki survei baik terhadap pembelajaran berjumlah 49 siswa (15,11%), siswa yang memiliki survei cukup terhadap pembelajaran berjumlah 227 Siswa (70,06%), dan siswa yang memiliki survei kurang terhadap pembelajaran berjumlah 48 Siswa (14,81%), presentase ini dapat dilihat di grafik Diagram pie dibawah.Bagi guru Guru diharapakan lebih memperhatikan siswa terutama pada semangat belajarnya agar siswa tidak merasa bosan saat melakukan pembelajaran PJOK. Bagi Siswa diharapkan sebelum melakukan proses pembelajaran berlagsung belajar terlebih dahulu agar dapat memberikan pemahaman awal sehingga saat masuk pelajaran lebih paham lagi dengan materi yang telah diajarkan.
Media Pembelajaran Shooting Futsal Menggunakan Media Target Hula Hoop Pada Siswa Kelas VIII Di SMP Negeri 56 Jakarta
Authors: Ardiyan Deni , Hasibuan Muchtar Hendra , Sukriadi Slamet , Irawan Andri , Hermanto Hermanto , Puspitorini Wahyuningtyas
Published: March 2024

Penelitian ini bertujuan untuk meningkatkan keterampilan teknik shooting futsal menggunakan media target hula-hoop sebagai media pembelajaran pada siswa kelas VIII di SMPN 56 Jakarta. Penelitian ini menggunakan metode penelitian tindakan kelas (PTK) dan analisa deskriptif komparatif. Sampel yang digunakan sebanyak 32 orang. Untuk mencapai tujuan dan keefektifannya, penelitian ini telah divalidasi oleh tiga pakar yaitu dosen ahli bidang pembelajaran, dosen ahli futsal, dan dosen ahli sarana dan prasarana pembelajaran. Dari hasil validasi, media pembelajaran berupa media target Hula hoop telah dinyatakan lulus tahap validasi ahli dan dapat diterapkan dalam pembelajaran shooting futsal di SMPN 56 Jakarta. Hasil penelitian menunjukkan bahwa penerapan media target hula hoop berpengaruh terhadap peningkatan hasil belajar siswa kelas VIII dalam pembelajaran teknik shooting futsal di SMPN 56 Jakarta. Hal ini dibuktikan dengan adanya peningkatan nilai dari tahap pra siklus (34%), siklus I (63%) dan siklus II (81%). Penelitian ini diharapkan dapat menjadi bahan referensi bagi guru dan mahasiswa yang akan mengembangkan media pembelajaran dalam pembelajaran teknik dasar shooting futsal di sekolah.
Efektifitas Tekhnik PNF Hold Relax dan Contract Relax Terhadap Peningkatan Fleksibilitas Otot Pinggang Atlet Tim Nasional Judo
Authors: Aziz Muhammad Fauzan , Hasibuan Muchtar Hendra , Prabowo Eko
Published: March 2024

Penelitian ini bertujuan untuk mengetahui persentase perbedaan efektifitas pnf hold relax dan pnf contract relax terhadap fleksibilitas otot pinggang pada atlet tim nasional judo. dengan menggunakan desain penelitian kuantitatif komparatif dan teknik eksperimen. Populasi dalam penelitian ini adalah atlet tim nasional judo Indonesia yang melakukan training center di ciloto, jawa barat, kemudian dilakukan pengambilan sampel menggunakan teknik sampling jenuh, dan didapatkan jumlah sampel sebanyak 32 atlet, yaitu mencari perbedaan dengan metode penelitian pre test dan post test. dilakukan 16 kali pertemuan untuk mendapatkan hasil skor penelitian, prosedur pengambilan data dilakukan dengan teknik observasi untuk mengetahui antara perbedaan metode pnf hold relax dan efek pnf contract relax. Hasil penelitian menunjukan bahwa adanya efek pnf hold relax mempengaruhi peningkatan fleksibilitas otot pinggang, dan efek pnf contract relax mempengaruhi peningkatan fleksibilitas otot pinggang, tetapi kedua efek tersebut tidak memiliki perbedaan signifikan terhadap peningkatan fleksibilitas otot pinggang terhadap atlet tim nasional judo.
STUDY ON AN AR-BASED CIRCUIT PRACTICE
Authors: Na Hae Chan , Kim Yoon Sang
Published: March 2024

Recently, the number of AR-based practice cases has been increasing. In this paper, the effect of AR-based circuit practice was examined through an experiment with 60 subjects (control group: 30, experimental group: 30). The report score, completion time, question count, and USE questionnaire were used in the analysis. As a result of the experiment, the report score was significantly increased by 15.48% in the experimental group (△report: +18 points) than in the control group (△report: +7 points). Question count decreased twice as much in the experimental group (△question: -18 times) as in the control group (△question: -9 times). The completion time of the experimental group (△time: -16 min) was reduced by approximately 4 min more than that of the control group (△time: -12 min), however, the difference was not statistically significant. The USE questionnaire received evaluations of 6.0 or higher (on a 7-point scale) in all categories (usefulness, ease of use, ease of learning, satisfaction). Therefore, From the experimental results, the proposed AR-based circuit practice is confirmed to be more effective than traditional circuit practice.
ENHANCING MEDICAL DATA SECURITY IN E-HEALTH SYSTEMS USING BIOMETRIC-BASED WATERMARKING
Authors: MAKHLOUF Ziadeddine , MERAOUMIA Abdallah , LAKHDAR Laimeche , HAOUAM Mohamed Yassine
Published: March 2024

In the field of Electronic Health (e-Health), Electronic Health Records (EHR) are transmitted between health professionals using e-Health systems for cooperative medical practice, medical monitoring, telemedical expertise, and telemedical imaging. Medical images are a crucial component of EHR and are used in various aspects of telemedicine systems such as expertise, consultation, teaching, and research. However, protecting the authenticity and copyrights of medical images is essential to prevent duplication, modification, or unauthorized distribution. This paper proposes a robust medical image copyright protection method that uses patient palm-print template as watermark and Lorenz chaotic map for template concealing and selecting the appropriate embedding positions in medical images. The novelty of the method lies in optimizing the expected number of modifications per pixel of the medical images after being watermarked. Experimental results indicate that this approach has a high performance with a genuine accept rate of 99.86% and can withstand various image processing attacks, including Gaussian noise, compression, and image rotations, while ensuring personal data security during telemedicine data exchange.
OPTIMIZING PEDESTRIAN TRACKING FOR ROBUST PERCEPTION WITH YOLOv8 AND DEEPSORT
Authors: Zidani Ghania , DJARAH Djalal , BENMAKHLOUF Abdslam , KHETTACHE Laid
Published: March 2024

Multi-object tracking is a crucial aspect of perception in the area of computer vision, widely used in autonomous driving, behavior recognition, and other areas. The complex and dynamic nature of environments, the ever-changing visual features of people, and the frequent appearance of occlusion interactions all impose limitations on the efficacy of existing pedestrian tracking algorithms. This results in suboptimal tracking precision and stability. As a solution, this article proposes an integrated detector-tracker framework for pedestrian tracking. The framework includes a pedestrian object detector that utilizes the YOLOv8 network, which is regarded as the latest state-of-the-art detector, that has been established. This detector provides an ideal detection base to address limitations. Through the combination of YOLOv8 and the DeepSort tracking algorithm, we have improved the ability to track pedestrians in dynamic scenarios. After conducting experiments on publicly available datasets such as MOT17 and MOT20, a clear improvement in accuracy and consistency was demonstrated, with MOTA scores of 63.82 and 58.95, and HOTA scores of 43.15 and 41.36, respectively. Our research highlights the significance of optimizing object detection to unleash the potential of tracking for critical applications like autonomous driving.
OPTIMIZING UNMANNED AERIAL VEHICLE BASED FOOD DELIVERY THROUGH VEHICLE ROUTING PROBLEM: A COMPARATIVE ANALYSIS OF THREE DELIVERY SYSTEMS.
Authors: Edirimanne Rumesh , Fernando W Madushan , Nielsen Peter , Perera H. Niles , Thibbotuwawa Amila
Published: March 2024

In recent times, there has been a notable increase in interest surrounding the integration of Un-manned Aerial Vehicle (UAV) technology and vehicle routing problems (VRP) for package delivery purposes. While existing studies have explored various types of package deliveries utilizing VRP, limited attention has been given to on-demand food delivery. This study aims to develop a VRP model that incorporates practical constraints such as payload capacity and maximum flying range, with the primary objective of minimizing travel distance in food delivery operations. A comparative analysis is conducted among three delivery methods, including UAV delivery, to determine the most effective approach and assess the feasibility of each method. Through a case study analysis focused on a pizza delivery service in Sri Lanka, it was observed that implementing VRP in a motorbike delivery system resulted in reduced travel distance, time, cost, and CO2 emissions compared to the existing delivery system. Furthermore, the utilization of UAVs in conjunction with VRP yielded even greater improvements across all parameters. Based on a comprehensive cost analysis considering long-term operations, the UAV-based delivery system was identified as the most cost-effective method, followed by the VRP-incorporated motorbike delivery method. Although the VRP-incorporated motorbike delivery system exhibited a slightly higher average time per route compared to the existing method, the total travel time required to complete all routes remained lower. Consequently, the study concludes that the VRP-incorporated motorbike delivery system outperforms the existing delivery method for food delivery, with the use of UAVs incorporating VRP identified as the optimal delivery method among the three alternatives. The findings contribute valuable insights to the optimization of food delivery logistics, emphasizing the potential of VRP and exploring the feasibility of UAVs for sustainable and efficient long-term delivery solutions.
KNOWLEDGE MANAGEMENT APPROACH IN COMPARATIVE STUDY OF AIR POLLUTION PREDICTION MODEL
Authors: ROHAJAWATI Siti , SETYODEWI Hutanti , TRESNANTO Ferryansyah Muji Agustian , MARIANTHI Debora , SIHOTANG Maruli Tua Baja
Published: March 2024

This study utilizes knowledge management (KM) to highlight a documentation-centric approach that is enhanced through artificial intelligence. Knowledge management can improve the decision-making process for predicting models that involved datasets, such as air pollution. Currently, air pollution has become a serious global issue, impacting almost every major city worldwide. As the capital and a central hub for various activities, Jakarta experiences heightened levels of activity, resulting in increased vehicular traffic and elevated air pollution levels. The comparative study aims to measure the accuracy levels of the naïve bayes, decision trees, and random forest prediction models. Additionally, the study uses evaluation measurements to assess how well the machine learning performs, utilizing a confusion matrix. The dataset’s duration is three years, from 2019 until 2021, obtained through Jakarta Open Data. The study found that the random forest achieved the best results with an accuracy rate of 94%, followed by the decision tree at 93%, and the naïve bayes had the lowest at 81%. Hence, the random forest emerges as a reliable predictive model for prediction of air pollution.
Determination of Micro Minerals of Several Species of Sea Urchins from Samboang Waters as Functional Food Candidates
Authors: Hartanti Lucky , Warsidah  , Helena Shifa , Tahirah  , Irwan
Published: March 2024

Functional foods are foods that can be consumed with additional health benefits beyond their basic function as an energy source. The search for food sources from the sea is increasing, with many discoveries of active compounds from marine organisms. This research aims to determine the micromineral content of iron (Fe), zinc (Zn), and iodine (I) in several species of sea urchins in the coastal waters of Samboang Bulukumba. The results of this research can be a basis for utilizing this biota as a functional food. This laboratory research uses micromineral measurement instruments, for example, Atomic Absorption Spectrophotometry (AAS) for Fe and Zn, while for iodine minerals using High Performed Liquid Chromatography (HPLC) instruments. In this study, 2 types of sea urchins were found which were identified as Diadema cytosum and Tripneustes gratilla. The results of measuring the water content and ash content of the gonads of the two sea urchins were 77.32% and 1.15% for Diadema cytosum and 72.22% and 2.09% for Tripneustes gratilla. The results of measuring the levels of micro minerals Fe, Zn, and Iodine were respectively 115.24 ppm, 31.44 ppm and 16.71 ppm for Diadema cytosum and 150.75 and 27.27 ppm 21.21 ppm for Tripneustes gratilla.
Keywords: functional food, AAS, HPLC, Diadema cytosum, Tripneustes gratilla
Abstrak
Pangan fungsional adalah makanan yang dapat dikonsumsi dengan manfaat kesehatan tambahan di luar dari fungsi dasarnya sebagai sumber energi. Pencarian sumber pangan dari laut semakin meningkat, dengan banyaknya penemuan senyawa-senyawa aktif dari organisme laut. Penelitian ini bertujuan  menentukan kandungan mikro  mineral besi (Fe), seng (Zn) dan yodium (I) dalam beberapa spesies bulu babi di perairan pantai Samboang Bulukumba. Hasil penelitian ini dapat menjadi dasar dalam memanfaatkan biota tersebut sebagai pangan fungsional. Penelitian laboratorium ini menggunakan instrumen pengukuran mineral mikro misalnya dengan  penggunaan Atomic Absorption Spectrofotometry (AAS) untuk Fe dan Zn, sedangkan untuk mineral yodium menggunakan instrumen High Performed Liquid Chromatography (HPLC). Dalam penelitian ini ditemukan 2 jenis bulu babi yang teridentifikasi sebagai  Diadema sitosum dan Tripneustes gratilla. Hasil pengukuran kadar air dan kadar abu gonad kedua bulu babi tersebut  diperoleh 77.32% dan 1.15% untuk Diadema sitosum dan sebesar 72.22% dan 2.09% untuk Tripneustes gratilla.  Hasil pengukuran  kadar mineral mikro Fe,  Zn dan Yodium masing-masing adalah sebesar 115.24  ppm, 31.44 ppm dan 16.71ppm untuk Diadema sitosum dan 150.75 dan 27.27 ppm 21.21 ppm untuk Tripneustes gratilla.
Kata kunci : pangan fungsional, AAS, HPLC, Diadema sitosum, Tripneustes gratilla
STRUKTUR KOMUNITAS DAN PERSENTASE TUTUPAN LAMUN DI DESA PINASUNGKULAN KECAMATAN TOMBARIRI KABUPATEN MINAHASA
Authors: Aleksander Vrenty Alvionita , Schaduw Joshian N.W. , Warouw Veibe , Windarto Agung B. , Wagey Billy Th. , Undap Suzanne L.
Published: March 2024

Indonesia is known as a maritime country that is rich in various marine biota, both flora and fauna, for example such as seagrass, in the world there are 60 species of seagrass and 12 species are found in Indonesia. This study aims to determine the community structure and percentage of seagrass cover in the waters of Pinasungkulan Village, Tombariri District, Minahasa Regency, this study used the seagrass watch method, observations were made at 2 station points (ST.1 and ST.2) using the equation formula from Cox, 1967. In the waters of Pinasungkulan Village there are 3 types of seagrass, namely Enhalus acoroides, Halodule pinifolia and Cymodocea rotundata. The percentage of seagrass cover in ST.1 was 22.54% and ST.2 was 28.22%, the average value obtained from these two stations was 25.38%. Overall, the community structure of ST.1 species Halodule pinifolia has the highest important value index of 143.46% and ST.2 species Cymodocea rotundata which has the highest important value index of 133.24%. Parameters in the waters of Pinasungkulan Village, Tombariri District, Minahasa Regency have a normal pH and a stable temperature with sand and silt as a substrate.

Keywords: seagrass, percentage cover, community structure

ABSTRAK

Indonesia dikenal sebagai negara maritim yang kaya dengan berbagai biota laut baik flora maupun fauna contonya seperti lamun, di dunia terdapat 60 jenis lamun dan 12 jenisnya terdapat di Indonesia. Dalam penelitian ini bertujuan untuk mengetahui struktur komunitas dan persentase tutupan lamun di perairan Desa Pinasungkulan Kecamatan Tombariri Kabupaten Minahasa, penelitian ini menggunakan metode seagrass watch, pengamatan dilakukan pada 2 titik stasiun (ST.1 dan ST.2) dengan menggunakan rumus persamaan dari Cox, 1967. Di perairan Desa Pinasungkulan terdapat 3 jenis lamun yaitu Enhalus acoroides, Halodule pinifolia dan Cymodocea rotundata. Hasil tutupan persentase lamun pada ST.1 yaitu 22,54% dan ST.2 yaitu 28,22%, nilai rata-rata yang diperoleh dari kedua stasiun ini yaitu 25,38%. Secara keseluruhan struktur komunitas dari ST.1 jenis Halodule pinifolia memiliki indeks nilai penting tertinggi yaitu 143,46% dan ST.2 jenis Cymodocea rotundata yang memiliki indeks nilai penting tertinggi yaitu sebanyak 133,24%. Parameter di periran Desa Pinasungkulan Kecamatan Tombariri Kabupaten minahasa memiliki pH normal dan suhu yang stabil dengan substrat pasir dan pasir lumpuran.

Kata kunci: lamun, Tutupan persentase, struktur komunitas
PENAPISAN BAKTERI SIMBION LAMUN Thalassia hemprichii PENGHASIL ENZIM HIDROLASE
Authors: Moroki Rizky I. , Ginting Elvy L. , Wullur Stenly , Tilaar , Sandra , Warouw Veibe , Ngangi Edwin L.A.
Published: March 2024

The research aims to determine the ability of the seagrass symbiont bacteria Thalassia hemprichii to produce protease and amylase. The symbiont bacteria T. hemprichii had been cultured in the laboratory. Five isolate bacteria namely 3M.2, E2.2, E3.2, 6.2 and 5.2 were analyzed in this research. Bacterial enzyme activity was screened by growing the bacterium using the paper disc on skim milk agar and starch agar media for 2 x 24 hours. Enzyme activity was indicated by the formation of a clear zone around the bacterial colonies. The proteolytic (IP) and amylolytic indexes (IA) were calculated by dividing the diameter of clear zone with the bacterium colonies. The results showed that the bacterial isolate 3M.2, E2.2 and 6.2 could produce proteases in 24 hours incubation with IP of 1,67, 1,20 and 1,33, respectively. While isolate 5.2 could produce protease after being incubated for 48 hours with an IP of 1,03. During the 48 hours incubation time, there were decrease in the IP of bacterial isolates of 3M.2, E2.2 and 6.2. Furthermore, 3M.2 bacterial isolate was able to produce amylase in 24 hours of incubation with an IA of 1,27. Meanwhile, bacterial isolate E2.2 could produce amylase after being incubated for 48 hours with an IA of 1,17.

Keywords: Seagrass T. hemprichii, hydrolase enzymes, amylase, protease

ABSTRAK

Penelitian ini bertujuan untuk menentukan kemampuan bakteri simbion lamun Thalassia hemprichii dalam menghasilkan enzim protease dan amilase. Sampel bakteri yang diuji merupakan bakteri yang telah dikultur di Laboratorium, berjumlah 5 isolat yakni 3M.2, E2.2, E3.2,

6.2 dan 5.2. Pengujian aktivitas enzim bakteri dilakukan dengan menumbuhkan bakteri pada media skim milk agar dan media amilum selama 2 x 24 jam dengan menggunakan metode kertas cakram. Aktivitas enzim ditandai dengan terbentuknya zona bening di sekitar koloni bakteri yang tumbuh, dan selanjutnya dihitung indeks proteolitik dan amilolitik. Hasil penelitian menunjukkan bahwa isolat bakteri 3M.2, E2.2 dan 6.2 dapat menghasilkan protease selama waktu inkubasi 24 jam dengan IP sebesar 1,67 dan 1,20 dan 1,33 secara berurutan. Sedangkan isolat 5.2 dapat menghasilkan protease setelah diinkubasi selama 48 jam dengan IP sebesar 1,03. Pada waktu inkubasi 48 jam, terjadi penurunan IP dari isolat bakteri 3M.2, E2.2 dan 6.2. Isolat bakteri 3M.2 mampu menghasilkan amilase pada waktu inkubasi 24 jam dengan IA sebesar 1,27, sedangkan isolat bakteri E2,2 dapat menghasilkan amilase setelah diinkubasi selama 48 jam dengan IA sebesar 1,17.

Kata Kunci : Lamun T. hemprichii, enzim hidrolase, amilase, proteas
AKTIVITAS ANTIBAKTERI DARI SPONS ASAL PERAIRAN PULAU BANTONG, BOLAANG MONGONDOW TIMUR
Authors: Losung Gian , Losung Fitje , Lintang Rosita A.J. , Tilaar Sandra O. , Wullur Stenly , Manoppo Henky
Published: March 2024

Sponges are one of the marine biota that produce bioactive compounds with various structural variations and one of their biological activities is as an antibacterial. This study aimed to examine the antibacterial activity of the crude sponge extract and fractions of the sponge fractionated by the liquid-liquid partition method. This antibacterial test was carried out on Staphylococcus aureus and Escherichia coli bacteria using the disc diffusion method (Kirby-Bauer method). The final result of this study showed that crude extracts from all types of sponge samples had antibacterial activity with the diameter of the inhibition zone on S. aureus bacteria media: Acanthostrongylophora ingens (14.3 mm), Liosina paradoxa (7.5 mm), Stylotella aurantium (14.1 mm); on media of E. coli bacteria: A. ingens (11.1 mm), L. paradoxa (7.3 mm), S. aurantium (12 mm). In the antibacterial test of the sponge fractions carried out on samples of S. aurantium sponges also showed that all fractions had antibacterial activity with the diameter of the inhibition zone on S. aureus bacterial media: N-hexane fraction (7.6 mm), ethyl acetate fraction (12 mm), methanol fraction (8 mm); on E. coli bacteria media: N-hexane fraction (9.3 mm), ethyl acetate fraction (15.5 mm), methanol fraction (8.5 mm).

Keywords: Sponge, Antibacterial, A. ingens, L. paradoxa, S. aurantium

 

ABSTRAK

 

Spons merupakan salah satu biota laut penghasil senyawa bioaktif dengan berbagai variasi struktur dan salah satu aktivitas biologisnya adalah sebagai antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak kasar spons dan fraksi-fraksi spons yang difraksinasi dengan metode partisi cair-cair. Pengujian antibakteri ini dilakukan terhadap bakteri Staphylococcus aureus dan Escherichia coli dengan menggunakan metode difusi cakram (metode Kirby-Bauer). Hasil akhir dari penelitian ini menunjukkan bahwa ekstrak kasar dari semua jenis sampel spons memiliki aktivitas antibakteri dengan diameter zona hambat pada media bakteri S. aureus: Acanthostrongylophora ingens (14.3 mm), Liosina paradoxa (7.5 mm), Stylotella aurantium (14.1 mm); pada media bakteri E. coli: A. ingens (11.1 mm), L. paradoxa (7.3 mm), S. aurantium (12 mm). Pada pengujian antibakteri fraksi-fraksi spons yang dilakukan pada sampel spons S. aurantium juga menunjukkan bahwa semua fraksi memiliki aktivitas antibakteri dengan diameter zona hambat pada media bakteri S. aureus: fraksi N-heksan (7.6 mm), fraksi etil asetat (12 mm), fraksi metanol (8 mm); pada media bakteri E. coli: fraksi N-heksan (9.3 mm), fraksi etil asetat (15.5 mm), fraksi metanol (8.5 mm).

Kata Kunci: Spons, Antibakteri, A. ingens, L. paradoxa, S. aurantium
ESTIMASI PENYERAPAN KARBON MANGROVE DI DESA PONTO KECAMATAN WORI KABUPATEN MINAHASA UTARA
Authors: Darman Firman , Sondak Calvyn F. A. , Rumengan Anthonius P. , Ompi Medy , Schaduw Joshian N. W. , Lohoo Anneke V.
Published: March 2024

Mangrove forest can be found grow along the coast, or river estuaries which ist growth and develop is influenced by tides. The mangrove forest plays an important role as carbon sequester to combat global warming. The purposes of this study are to calculate the potential biomass of mangrove trees in the Ponto village and to estimate carbon stocks (C) storage and carbon dioxide (CO2) uptake by mangrove stands in Ponto village. The data collection method used in this research is the line transect quadratic method without damaging the object of the study. The data taken were mangrove tree biomass data. The data collection was carried out in 12 plots measuring (10 x 10 m2).All trees found within the plots were recorded for their number, type and diameter at breast height(DBH).This study found that a total of five mangrove spesies, namely: Rhizophora apiculata, Avicennia officinalis, Xylocarpus granatum, Bruguiera gymnorrhiza, and Ceriops tagal. Tthe highest density value was 1,500 trees/ha. Total tree biomass was 64,64-105,23 ton/ha, estimated carbon(C) content and carbon dioxide (CO2) mangrove were 30,38-49,46 ton C/ha to111,50- 181,51 ton CO2/ha respectively.

Keywords : Mangrove, density, diameter, carbon, carbon dioxide

 

ABSTRAK

Hutan mangrove adalah tipe hutan yang khas terdapat di sepanjang pantai atau muara sungai yang tumbuh dan berkembangnnya dipengaruhi oleh pasang surut air laut. Hutan mangrove memiliki salah satu fungsi ekologis yaitu berperan dalam upaya mitigasi pemanasan global karena hutan mangrove sebagai penyerap dan penyimpan karbon dioksida (CO2) yang dilakukan melalui proses fotosintesis dan menyimpannya dalam stok biomasa. Tujuan dari penelitian ini ada 2 yaitu: (1) menghitung potensi biomasa pada pohon mangrove di Desa Ponto (2) mengestimasi simpanan karbon (C) dan serapan karbondioksida (CO2) atas tegakan mangrove di Desa Ponto. Metode pengambilan data digunakan dalam kegiatan penelitian ini metode line transek kuadrat dilakukan tanpa merusak objek penelitian. Data yang diambil adalah data biomasa pohon mangrove. Untuk pengambilan data dilakukan dalam 12 plot berukuran 10 x 10 m2. Semua pohon yang ada di dalam plot dicatat jumlah, jenis serta diukur DBH (diameter at breast height) dari keseluruhan sampel. Hasil penelitian diperoleh total pohon mangrove yang teridentifikas 5 jenis yaitu: Rhizophora apiculata, Avicennia officinalis, Xylocarpus granatum, Bruguiera gymnorrhiza, dan Ceriops tagal dengan nilai kerapatan tertinggi 1,500pohon/ha.Dari hasil perhitungan diperoleh total jumlah biomasa berkisar 64,64-105,23 ton/ha, serta hasil estimasi kandungan karbon (C) dan karbon dioksida (CO2) tegakan mangrove sebesar 30,38-49,46ton C/ha atau setara111,50-181,51ton CO2/ha.

Kata Kunci: Mangrove,Kerapatan,diameter,karbon,karbon dioksida
KEPADATAN DAN KEANEKARAGAMAN JENIS FAUNA BENTOS (>1MM) PADA DAERAH PECAHAN KARANG DI PERAIRAN KELURAHAN MOLAS TELUK MANADO
Authors: Hanibe Jodi J. , Mamangkey Noldy G. F. , Manembu Indri S. , Boneka Farnis B. , Ompi Medy , Pangemanan Novie P.L.
Published: March 2024

Benthic fauna is a group of benthic organisms that live on the bottom of the water or the bottom of the sediment or between sediments. This study aims to obtain an overview of the distribution and types of benthic faunal organisms measuring > 1mm in the waters of Molas Village at a depth of 1-3 m, especially around coral fragments. Benthos sampling was carried out using a grab sampler with three repetitions. The benthic sediment sample was sieved using a 1000 m (1.0 mm) sieve.  The sediment retained in the sieve was then identified based on its morphological characters using a  stereo microscope.  Furthermore, the number of organisms found was counted and analyzed according to the calculation of the ecological index. The results of the identification of benthic faunal organisms >1mm obtained a total of 36 types of mollusks consisting of 34 species belonging to the class Gastropods and 2 species belonging to the class Bivalvia which were divided into 24 families and obtained an average density of 81.4 ind/m2, Diversity Index 1, 47 (medium category), Uniformity Index 0.97 (high category) and Dominance Index 0.27 (nothing dominates).

Keywords: benthic ecology, grab sampling, Manado Bay

 

ABSTRAK

Fauna bentos merupakan kelompok organisme bentos yang hidup di dasar perairan atau dasar sedimen maupun di antara sedimen. Penelitian ini bertujuan untuk mendapatkan gambaran distribusi dan jenis organisme fauna bentos yang berukuran > 1mm di daerah perairan Kelurahan Molas padakedalaman 1-3 m khususnya di sekitar pecahan karang. Pengambilan sampel bentos dilakukan dengan menggunakan grab sampler dengan pengulangan sebanyak tiga kali. Sampel sedimen bentos diayak menggunakan saringan 1000 µm (1,0 mm). Sedimen yang tertahan di saringan kemudian diidentifikasi berdasarkan karakter morfologi dengan menggunakan bantuan mikroskop stereo. Selanjutnya jumlah organisme yang ditemukan dihitung dan dianalisis menurut perhitungan indeks ekologi.Hasil identifikasi organisme fauna bentos >1mm mendapatkan total 36 jenis moluska yang terdiri dari 34 spesies anggota kelas Gastropoda dan 2 spesies anggota kelas Bivalvia yang terbagi dalam 24 famili dan mendapatkan hasil rata-rata kepadatan 81,4 ind/m2, Indeks Keanekaragaman 1,47 (kategori sedang), Indeks Keseragaman 0,97 (kategori tinggi) dan Indeks Dominansi 0,27 (tergolong tidak ada yang mendominasi).

Kata Kunci: Ekologi bentos, grab sampling, Teluk Manado
TOXICITY TEST OF SOFTCORAL Lobophytum Sp AGAINST Artemia salina SHRIMP, L USING THE BRINE SHRIMP LETHALITY METHOD
Authors: Kawung Nickson J. , Rompas Rizald M. , Wagey Billy T. , Sumangando Adolfina , Kaempe Hindang , Untu Sonny , Palandi Royke R.
Published: March 2024

Marine biota resources as a potential asset that can be used into various products including pharmaceutical products because they were a natural ingredient that very rich in biologically active compounds with a unique structure. Softcoral Lobophytum is one of the marine biota that has the secondary metabolites which can be useful in pharmacology field. The purpose of this study is to test the toxicity of the soft coral extract of Lobophytum sp as an anti-cancer biopotential. This research is using the Brine Shrime Lethality Test (BSLT) method, with laboratory experiments. Solfcoral samples were taken from Bunaken Island that using purposive sampling method. The test concentrations using 10, 100, 500 and 1000 ppm. The analysis toxicity using probit analysis for minitab program. The results showed that the increasing of concentration was  followed by the increasing of the mortality number of the animal testing. The results of probit analysis obtained an LC50 value of 9.98 ppm. These results indicate that the bioactive substance of softcoral Lobophytum sp has the potential as an anti-cancer compound.

Keywords: Soft coral, Lobophytum sp, anti cancer, Artemia salina

 

ABSTRAK

Sumber daya biota laut merupakan aset potensial yang dapat digunakan menjadi aneka produk termasuk di antaranya produk farmasi karena merupakan bahan alam yang sangat kaya senyawa aktif biologi dengan struktur yang unik. Softcoral Lobophytum salah satu biota laut yang memiliki metabloit sekunder yang dapat bermanfaat dalam bidang farmakologi. Tujuan dari penelitian ini bertujuan untuk menguji toksisitas ekstrak karang lunak Lobophytun sp sebegai biopotensi antikanker. Metode penelitiaan digunakan yaitu metode Brine Shrime Lethality  Tes (BSLT), dengan percobaan laboratorium. Sampel solfcoral diambil dari pulau Bunaken, pengambilan sampel menggunakan metode purposive sampling. Konsentrasi uji menggunakan 10, 100, 500 dan 1000 ppm. Analisis Toksisitas menggunakan analisis probit dengan  menggunakan program minitab. Hasil yang diperoleh menunjukan setiap bahwa setiap kenaikan konsentrasi diikuti dengan kenaikan jumlah mortalitas hewan uji. Hasil analisis probit diperoleh nilai LC50 9.98 mg/l. Hasil ini menunjukan bahwa susbtans bioaktif darri softcoral Lobophytum sp berpotensi sebagai senyawa antikanker.

Kata kunci. Karang lunak, Lobophytum sp, anti kanker, Artemia salina
ESTIMASI KANDUNGAN KARBON SERASAH DAUN MANGROVE RHIZOPHORA spp. DI HUTAN MANGROVE DESA WORI, KECAMATAN WORI, KABUPATEN MINAHASA UTARA
Authors: Rerung Ekarisma , Sondak Calvyn F. A. , Bara Robert A. , Darwisito Suria , Paruntu Carolus P. , Tombokan John L.
Published: March 2024

Mangroves play an important role in supporting life in coastal and marine areas. As plants that live in marine and land areas, mangrove forests transfer various kinds of nutrients both on land and into the sea. Decreasing area of mangrove forests leads to a decrease in mangrove litter production. Mangrove litter has a function as a carbon sink and storage. The purpose of this study was to determine the percentage of carbon content (C) and estimate the amount of carbon stored in the leaf litter of mangrove Rhizophora spp., in the mangrove forest of Wori Village, Wori District, North Minahasa Regency. The Litter-trap method (litter trapping net) was used in this study. Litter collection was carried out for 28 days in 12 litter-traps measuring 1x1 m2. All litters trapped in the litter-trap were weighed in their wet and dry weight, then analyzed in the laboratory to determine the carbon content. Based on the results of the analysis, the total average percentage of carbon content in mangrove litter 25.93% C, and from the estimation results, the average carbon content (C) in the litter is 19.99 tons/ha/year.

Keywords: Mangrove, Litter, Litter-Trap, Carbon Content, Wori Village

ABSTRAK

Mangrove merupakan ekosistem yang berperan penting dalam mendukung kehidupan di wilayah pesisir dan kelautan. Sebagai tumbuhan yang hidup di wilayah laut dan darat, hutan mangrove mentransfer berbagai macam nutrien baik ke darat maupun ke laut. Berkurangnya luasan hutan mangrove menyebabkan produksi serasah mangrove juga berkurang. Serasah mangrove memiliki fungsi sebagai penyerap dan penyimpan karbon.Adapun tujuan dari penelitian ini yaitu untuk mengetahui persentase kandungan karbon (C) dan Mengestimasi jumlah karbon yang tersimpan pada daun serasah mangrove Rhizophora spp., di hutan mangrove Desa Wori, Kecamatan Wori Kabupaten Minahasa Utara.Pengambilan sampel pada penelitian ini menggunakan metode Litter- trap (jaring penangkap serasah). Pengumpulan serasah dilakukan selama 28 hari dalam 12 Litter-trap yang berukuran 1x1 m2 . Seluruh serasah yang terperangkap di dalam litter-trap di timbang berat basah dan berat keringnya, kemudian dilakukan analisis di Laboratorium untuk mengetahui kandungan karbon. Berdasarkan hasil analisis didapatkan total rata-rata persentase kandungan karbon serasah mangrove yakni sebesar 25,93 % C, serta dari hasil estimasi didapatkan rata-rata kandungan karbon (C) pada serasah sebesar 19,99 ton/ha/tahun.

Kata Kunci: Mangrove, Serasah, Litter-Trap, Kandungan Karbon, Desa Wori
ISOLASI DAN UJI AKTIVITAS ANTI BAKTERI DARI JAMUR SIMBION DARI TERIPANG (Holothuroidea sp.) YANG DIAMBIL DI PERAIRAN KELURAHAN MOLAS KECAMATAN BUNAKEN PROVINSI SULAWESI UTARA
Authors: Mamangkey Epsan J. , Losung Fitje , Bara Robert. A. , Angmalisang Ping Astony , Rumampuk Natalie D.C. , Tumbol Reiny
Published: March 2024

Sea cucumbers are one of the marine biotas which consist of high nutritional and protein content. One bioactive compound in sea cucumbers is triterpenoid with antifungal and anti -bacterial properties. This study aimed to obtain fungal symbionts derived from marine cucumbers from Molas Waters, North Sulawesi continued by extraction and testing their antibacterial property towards bacterial strains Escherichia coli and Staphylococcus aureus. The antibacterial activity test was carried out by using the Kirby-Bauer disc diffusion method. In the results, we found 3 species of sea cucumbers from Molas Waters, e. g. Holothuria atra, Bohadschia argus and Holothuria scabra. From these species, three isolates of fungal symbionts were isolated and tested for their antibacterial property. These three extracts array antibacterial activity against E. coli and S.aureus. The most potent antibacterial activity was found in the extract of fungal symbiont from H. atra with an average of 29.3 mm against E.coli and

28.0 mm against S.aureus.

 

Keywords: Sea cucumber, antibacterial activity test, Escherichia coli

ABSTRAK

Teripang adalah salah satu biota yang memiliki kandungan gizi dan protein yang juga cukup tinggi dalam tubuh teripang Salah satu senyawa yang terkandung dalam teripang yaitu senyawa triterpenoid yang memiliki aktivitas sebagai anti jamur dan anti bakteri. Tujuan dari penelitian ini yaitu untuk mendapatkan isolat jamur dan ekstrak jamur yang bersimbiosis dengan teripang dan menguji aktivitas antibakteri terhadap strain Escherichia coli dan Staphylococcus aureus dengan metode difusi agar (disc diffusion Kirby-Bauer). Hasil penelitian didapatkan dari 3 jenis teripang dari Pantai Molas yaitu Holothuria atra, Bohadschia argus dan Holothuria scabra. Dari ketiga spesies teripang ini  telah diisolasi tiga isolat jamur simbion untuk diuji aktivitas antibakterinya. Ketiga ekstrak jamur simbion asal teripang ini memiliki aktivitas antibakteri terhadap strain bakteri Escherichia coli dan Staphylococcus aureus. Aktivitas antibakteri yang paling kuat dari terdapat pada ekstrak jamur simbion asal Holothuria atra dengan rata-rata 29,3 mm terhadap bakteri Escherichia coli dan 28,0 mm terhadap bakteri Staphylococcus aureus.

Kata kunci : Teripang laut, Uji aktivitas antibakteri, Escherichia coli
KOMUNITAS DAN KEANEKARAGAMAN ASCIDIA DI PESISIR MINANGA, MALALAYANG SATU, KOTA MANADO
Authors: Palit Christian , Sumilat Deiske A. , Rumengan Antonius P. Rumengan , Boneka Farnis B. , Sinjal Chatrien A. L. , Lalita Jans
Published: March 2024

Ascidian is one of the biota components that make up coral reefs, which has many  biological, ecological and pharmaceutical benefits. This makes Ascidian a very attractive target due to its high and unique diversity among marine invertebrates. The purpose of this study is to provide information about the Ascidia community and diversity in the Minanga Coast, Malalayang Satu, Manado City. This research was conducted for 3 (three) months from April to June 2022 with the location of data collection is Minanga Divers Coast. Data were collected using the visual census method at a depth of 7 and 14 m with a transect length of 50 m and a width of 5 m. Data analysis was carried out to obtain the value of species composition and density and ecological index. The results obtained: 1). Ascidia found were 14 species consisting of 12 species at a depth of 7 m and 10 species at a depth of 14 m. 2). The highest species composition and density values were Ascidia P. aurata, and then D. molle 3). The Ascidia diversity index at the study site was was 2.00 at a depth of 7 m and 1.84 at a depth of 14 m.

Keyword : Ascidian, species composition, density, diversity.

ABSTRAK

Ascidia merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai banyak manfaat baik biologi, ekologi dan farmasitika. Sehingga menjadikan Ascidia target yang sangat menarik karena keanekaragamannya yang tinggi dan unik di antara avertebrata laut. Tujuan penelitian ini yaitu memberikan informasi mengenai komunitas Ascidia di Pesisir Minanga, Malalayang Satu, Kota Manado. Penelitian ini dilakukan selama 3 (tiga) bulan April – Juni 2022 dengan lokasi pengambilan data yaitu Pesisir Minanga Divers. Pengambilan data dilakukan dengan menggunakan metode sensus visual pada kedalaman 7 dan 14 m dengan panjang transek 50 m dan lebar 5m. Analisis data dilakukan untuk mendapatkan nilai komposisi jenis dan kepadatan, indeks ekologi dan pola sebaran. Hasil yang didapatkan : 1). Ascidia yang ditemukan berjumlah 14 spesies yang terdiri dari 12 spesies pada kedalaman 7 m dan 10 spesies pada kedalaman 14  m, 2). Nilai komposisi jenis dan kepadatan tertinggi yaitu Ascidia P. aurata pada D. molle da 3). Indekskeanekaragaman Ascidia pada lokasi penelitian yaitu dengan nilai 2,00 pada kedalaman 7 m dan 1,84 padakedalaman 14 m.

Kata Kunci : Ascidia. komposisi spesies, kepadatan, keanekaragaman.