bluuebunny/crossref_metadata_2025 huggingface dataset:
A nationwide analysis of the treatment patterns, survival, and medical costs in Korean patients with relapsed or refractory diffuse large B-cell lymphoma
Authors: Cho Jeong-Yeon , Jang Suk-Chan , Kang Dong-Won , Lee Eui-Kyung , Koh Hyein , Yoon Dok Hyun , Park Mi-Hai
Published: February 2024

Background

Approximately one-third of patients with diffuse large B-cell lymphoma (DLBCL) are refractory to treatment or experience relapse after initial therapy. Unfortunately, treatment options for older patients and those who experience relapse or become refractory to hematopoietic stem cell transplantation (HSCT) are limited. This nationwide population-based study aimed to identify treatment patterns, survival times, and treatment costs in patients with relapsed/refractory DLBCL (R/R DLBCL).

Materials and methods

Between 2011 and 2020, data on patients with R/R DLBCL were retrieved from the Korean Health Insurance Review & Assessment Service, encompassing the entire population. We identified the treatment patterns for each treatment line using a Sankey diagram and calculated the median time to the subsequent treatment in line. Median overall and progression-free survival times were estimated using the Kaplan–Meier survival curves. Finally, the medical costs incurred during DLBCL treatment were calculated for each treatment line and the costs related to HSCT were summarized at the episode level.

Results

A total of 864 patients with R/R DLBCL who received second-line treatment were identified, and a regimen of ifosfamide, carboplatin, and etoposide (ICE) was administered the most. Among them, 353 were refractory or relapsed cases that were treated with third-line treatments. The median times for second-line to third-line, third-line to fourth-line, fourth-line to fifth-line, and fifth-line to sixth-line treatment failures gradually decreased (3.93, 2.86, 1.81, and 1.38 months, respectively). The median overall survival time was 8.90 and 4.73 months following the second-line and third-line treatments, respectively. In the third-line treatment setting, the patients did not show a significant difference in survival time after HSCT. The median medical cost was $39,491 across all treatment lines including the cost of HSCT which was $22,054.

Conclusion

The treatment patterns in patients with R/R DLBCL, especially at third-line treatments and thereafter, were complicated, and their prognosis was poor despite the high medical costs. Novel and effective treatment options are expected to improve the prognosis and alleviate the economic burden of patients with R/R DLBCL.
The functional decline of tomato plants infected by Candidatus Liberbacter solanacearum: an RNA-seq transcriptomic analysis
Authors: Chuan Jiacheng , Nie Jingbai , Cooper William Rodney , Chen Wen , Hale Lawrence , Li Xiang
Published: February 2024

Introduction

Candidatus Liberibacter solanacearum (CLso) is a regulated plant pathogen in European and some Asian countries, associated with severe diseases in economically important Apiaceous and Solanaceous crops, including potato, tomato, and carrot. Eleven haplotypes of CLso have been identified based on the difference in rRNA and conserved genes and host and pathogenicity. Although it is pathogenic to a wide range of plants, the mechanisms of plant response and functional decline of host plants are not well defined. This study aims to describe the underlying mechanism of the functional decline of tomato plants infected by CLso by analyzing the transcriptomic response of tomato plants to CLso haplotypes A and B.

Methods

Next-generation sequencing (NGS) data were generated from total RNA of tomato plants infected by CLso haplotypes A and B, and uninfected tomato plants, while qPCR analysis was used to validate the in-silico expression analysis. Gene Ontology and KEGG pathways were enriched using differentially expressed genes.

Results

Plants infected with CLso haplotype B saw 229 genes upregulated when compared to uninfected plants, while 1,135 were downregulated. Healthy tomato plants and plants infected by haplotype A had similar expression levels, which is consistent with the fact that CLso haplotype A does not show apparent symptoms in tomato plants. Photosynthesis and starch biosynthesis were impaired while starch amylolysis was promoted in plants infected by CLso haplotype B compared with uninfected plants. The changes in pathway gene expression suggest that carbohydrate consumption in infected plants was more extensive than accumulation. In addition, cell-wall-related genes, including steroid biosynthesis pathways, were downregulated in plants infected with CLso haplotype B suggesting a reduction in membrane fluidity, cell signaling, and defense against bacteria. In addition, genes in phenylpropanoid metabolism and DNA replication were generally suppressed by CLso infection, affecting plant growth and defense.

Discussion

This study provides insights into plants’ defense and functional decline due to pathogenic CLso using whole transcriptome sequencing and qPCR validation. Our results show how tomato plants react in metabolic pathways during the deterioration caused by pathogenic CLso. Understanding the underlying mechanisms can enhance disease control and create opportunities for breeding resistant or tolerant varieties.
Measurement of flourishing: a scoping review
Authors: Rule Andrew , Abbey Cody , Wang Huan , Rozelle Scott , Singh Manpreet K.
Published: February 2024

Introduction

Flourishing is an evolving wellbeing construct and outcome of interest across the social and biological sciences. Despite some conceptual advancements, there remains limited consensus on how to measure flourishing, as well as how to distinguish it from closely related wellbeing constructs, such as thriving and life satisfaction. This paper aims to provide an overview and comparison of the diverse scales that have been developed to measure flourishing among adolescent and adult populations to provide recommendations for future studies seeking to use flourishing as an outcome in social and biological research.

Methods

In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), we conducted a scoping review across PubMed and EMBASE of studies introducing original flourishing scales (defined as a previously unpublished measure of mental health or wellbeing that used “flourishing” in its definition). Studies focusing on adult populations that were published before April 28, 2023 were considered eligible for inclusion.

Results

Out of 781 studies retrieved, we identified seven eligible studies covering seven unique flourishing scales. We find that all seven scales are multidimensional and assess features over monthly or yearly intervals. While most of the scales (six out of seven) include indicators of both hedonic and eudaimonic wellbeing, the operationalization of these dimensions of wellbeing varies considerably between scales. Several of the scales have been translated and validated across multiple geographical contexts, including higher- and lower-income countries.

Discussion

Complementing self-report measures with other social, economic, regional, and biological indicators of flourishing may be useful to provide holistic and widely applicable measures of wellbeing. This review contributes to concept validation efforts that can guide strategies to sustain flourishing societies.
Should the existing science of teams be applied to fluid teams? An exploration of fluid team effectiveness within the context of healthcare simulation
Authors: Grossman Rebecca , Billotti Brianna M. , Ha Joseph J. , Cassara Michael
Published: February 2024

Introduction

Fluid teams have become increasingly prevalent and necessary for modern-day issues, yet they differ from more traditional teams, on which much of the current teams literature is based. For example, fluid teams are often comprised of members from different disciplines or organizational divisions who do not have a shared history or future, as they come together to perform a critical, time-sensitive task, and then disband. For these reasons, the mechanisms through which they function and perform may differ from those of more traditional teams, and research is needed to better understand these differences.

Methods

To this end, this study utilized critical incident techniques and thematic analysis to examine fluid teams within healthcare, one of the primary contexts in which they are prevalent. Interdisciplinary faculty and students in the medical field who encounter fluid teams within simulation-based education were prompted to reflect on key factors that facilitate or hinder fluid team effectiveness.

Results

Primary themes extracted pertained to the conditions fluid teams operate within (e.g., high-stress), the behaviors and emergent states that contribute to their success (e.g., communication), and the KSAO’s of value for members of fluid teams to possess (e.g., readiness). These themes were then compared to existing literature, yielding the identification of some similarities but also many important differences between fluid and traditional teams.

Discussion

A series of practical recommendations for how to promote fluid team effectiveness is then presented.
Attachment to robots and therapeutic efficiency in mental health
Authors: Szondy Mate , Fazekas Peter
Published: February 2024

Previous studies in the mental health context have demonstrated that interactions with social robots can improve the mood and cognitive capacities of their users, and enhance their quality of life. In this Perspective article, our goal is to systematize the possible roles of social robots and to point out that different roles require different levels of attachment. We argue that the attachment between the client and the (robot) therapist is a fundamental ingredient of any helping relationship and that the full potential of using social robots in mental health settings can only be realized if the strength of attachment is appropriately correlated with the type of relationship established.
Case report: First documented case of cerebral angiostrongyliasis caused by Angiostrongylus costaricensis in a free-ranging opossum
Authors: Solorzano-Scott Tamara , Aguilar-Vargas Fernando , Cordero-Salas Martha , Conejo Amanda , Rojas Alicia , Baldi Mario
Published: February 2024

Angiostrongylus costaricensis is a metastrongyloid nematode that primarily infects the mesenteric arteries of wild rodents. This parasite is endemic in several regions of the American continent, and in humans, causes a disease known as abdominal angiostrongyliasis. Despite the important health implications of this nematode, there are limited studies investigating the involvement of wild animals in its life cycle. In this study, we present the clinical manifestations, pathologic findings, and molecular diagnosis, to the best of our current knowledge, of the first documented onset of cerebral angiostrongyliasis because of A. costaricensis infection in a juvenile free-ranging opossum (Didelphis marsupialis). Histopathological findings stress the presence of eosinophilic meningoencephalitis with nematodes present within the lesions, and PCR was positive for cox1 and ITS1 reactions. The obtained sequences for a 279 bp fragment of ITS1 were 100% identical to A. costaricensis from Costa Rica. This case highlights the substantial difficulties in diagnosing neuroangiostrongyliasis, yet underscores the importance of considering A. costaricensis as a potential culprit behind neurological conditions in wild marsupials. It acts as an urgent call to action to improve surveillance programs tracking infectious and parasitic diseases causing mortality in wildlife populations.
Large-scale serological survey on Mycobacterium avium subsp. paratuberculosis infection in sheep and goat herds in Sicily, Southern Italy
Authors: Di Marco Lo Presti Vincenzo , Ippolito Dorotea , Migliore Sergio , Tolone Marco , Mignacca Sebastian Alessandro , Marino Anna Maria Fausta , Amato Benedetta , Calogero Rosita , Vitale Maria , Vicari Domenico , Ciarello Flavia Pruiti , Fiasconaro Michele
Published: February 2024

Introduction

Paratuberculosis (PTB) is a worldwide chronic, contagious enteric disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) mainly affecting ruminant species. PTB is a WOAH-listed disease with direct and indirect economic losses in the livestock sector, negative impact on animal welfare and significant public health concerns. In spite of this, MAP prevalence in small ruminants is still unknown and the prevalence appears to be underestimated in many countries. The aim of this study is providing a first large-scale serological survey on MAP infection in small ruminants in Sicily, a region of Southern Italy with the 11.3 and 8.9% Italian national heritage of sheep and goats, respectively.

Methods

For this purpose, we analyzed a total of 48,643 animals reared in 439 flocks throughout Sicily. MAP seroprevalence was estimated both at herd-level and animal-level within breeds reared in all the nine sampled provinces.

Results

Our results revealed a high overall apparent prevalence at herd-level of 71.8% in sheep and 60.8% in goat farms with an animal-level prevalence of 4.5 and 5.1% in sheep and goats, respectively. Significant statistical differences were found between the provinces and within the breeds both in sheep and goats.

Discussion

Our study provides the first large-scale serological survey on PTB infection in small ruminants in Sicily and showed a high prevalence of disease depending to the species, breed and province. This study represents the first step to better understand the MAP epidemiology in a typical Mediterranean breeding context, suggesting the need of in-depth study on the herds risk factors, including the eventual presence of candidate genes for resistance/susceptibility to PTB in native breeds.
Role of hazelnut skin supplementation on plasma antioxidant status and cytokine profile in growing lambs
Authors: Ciliberti Maria Giovanna , Santillo Antonella , Caroprese Mariangela , della Malva Antonella , Natalello Antonio , Bertino Antonino , Albenzio Marzia , Sevi Agostino
Published: February 2024

In this study, the effect of hazelnut skin dietary supplementation on antioxidant status and cytokine profile was evaluated in growing lambs. A total of 22 male lambs at the age of 2 months, balanced for their initial live weight (15.33 ± SD 1.79 kg), were selected and allocated into two experimental groups: the control group (CON) receiving a maize-barley-based concentrated diet, and the hazelnut group (HS) receiving supplementation with hazelnut skin (150 g/kg on the dry matter) as a maize substitute for the concentrate diet. The experiment lasted for 56 days. Peripheral blood was collected at 7, 35, and 56 days of the experiment. The free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, the total antioxidant capacity assay (TAC), the reactive oxygen species (ROS), and reactive nitrogen species (RNS) were determined in plasma. The secretion of IL-1β, IL-6, and IL-10 cytokines was also determined by ELISA. The DPPH was affected by the interaction between feeding strategy and time of sampling (p = 0.039) with a higher level of DPPH at 7 days in the HS group than the CON group. The time of sampling affected the levels of plasma TAC (p = 0.016), while the ROS/RNS levels showed a higher value in the HS group (p < 0.001), on average. The antioxidant/oxidant index, which combines the TAC and the ROS/RNS levels, was not affected by the inclusion of hazelnut skin in the diet (p = 0.394). The cytokine profile showed a lower IL-6 secretion at both 35 and 56 days than at 7 days, on average. Furthermore, the feeding treatment affected the IL-1β level, showing a lower level in the HS group than in the CON group on average. Lambs from the HS group had higher IL-10 plasma levels than the CON group at 7 days of the experiment. The present data highlight an antioxidant effect and a modulatory role in the cytokine profile of HS supplementation in growing lambs.
Effect of Double-Ovsynch and Presynch-Ovsynch on postpartum ovarian cysts and inactive ovary in high-yielding dairy cows
Authors: Li Ziyuan , Luan Shuyi , Yan LongGang , Xie Chengyun , Lian Zhengjie , Yang Mingmao , Mei Minmin , Lin Pengfei , Wang Aihua , Jin Yaping
Published: February 2024

Introduction

Optimizing the management of dairy cattle reproduction can reduce postpartum ovarian disease in high-yielding dairy cows and thus enhance ranch economic benefit. The hypothesis of this study was that the Double-Ovsynch (DO) protocol in high-producing dairy cows would result in a lower incidence of follicular cysts but a higher incidence of luteal cysts compared to those undergoing the Presynch-Ovsynch (PS) protocol.

Methods

In this experiment, 384 cows (204 primiparous and 180 multiparous) were allocated to the DO group, which followed the protocol: GnRH-7d-PGF2α-3d-GnRH-7d-Ovsynch-56 h (GnRH-7d-PGF2α-56 h-GnRH-16hTAI), starting on 39 ± 3 days in milk (DIM). Additionally, 359 cows (176 primiparous and 183 multiparous) were assigned to the PS group, which followed the protocol: PGF2α-14d-PGF2α-12d-Ovsynch-56 h, starting on 31 ± 3 DIM. In DO, B-mode ultrasound examinations were conducted 1 day after the GnRH-7d-PGF2α-3d-GnRH protocol to diagnose the presence of ovarian diseases followed by reexamination after 7 days of suspected cases. In PS, B-mode ultrasound examinations were conducted 1 day after the PGF2α-14d-PGF2α protocol to diagnose the presence of ovarian diseases followed by reexamination after 7 days. For all cows confirmed to having ovarian diseases, a second B-mode ultrasound examination was conducted at the time of the second GnRH and timed artificial insemination (TAI). If the ovary showed a normal developing follicle in combination with normal ovulation, the ovarian disease was considered to be cured.

Results

The current study revealed no significant difference in the overall incidence and cure rate of postpartum ovarian diseases between DO and PS (incidence rate: 3.9% vs. 6.7%, cure rate: 50% vs. 41.7%, DO vs. PS). Also, there was no significant difference in the incidence and cure rate of luteal cysts between DO and PS (incidence rate: 2.9% vs. 2.2%, cure rate: 50.0% vs. 50.0%). The incidence of follicular cysts was significantly lower in the DO group than in the PS group (0.8% vs. 2.8%, DO vs. PS, p = 0.037), but there was no significant difference in the cure rates (66.7% vs. 50%). The occurrence of inactive ovary was lower in DO compared to PS (0.2% vs. 1.7%, p = 0.047). There was no significant difference in the pregnancy rate between the DO and PS groups (48.2% vs. 41.8%), although the DO group had a higher rate. What is different from our assumption is that PS did not effectively reduce the incidence of postpartum luteal cysts.
Enhanced Intrusion Detection Systems Performance with UNSW-NB15 Data Analysis
Authors: More Shweta , Idrissi Moad , Mahmoud Haitham , Asyhari A. Taufiq
Published: February 2024

The rapid proliferation of new technologies such as Internet of Things (IoT), cloud computing, virtualization, and smart devices has led to a massive annual production of over 400 zettabytes of network traffic data. As a result, it is crucial for companies to implement robust cybersecurity measures to safeguard sensitive data from intrusion, which can lead to significant financial losses. Existing intrusion detection systems (IDS) require further enhancements to reduce false positives as well as enhance overall accuracy. To minimize security risks, data analytics and machine learning can be utilized to create data-driven recommendations and decisions based on the input data. This study focuses on developing machine learning models that can identify cyber-attacks and enhance IDS system performance. This paper employed logistic regression, support vector machine, decision tree, and random forest algorithms on the UNSW-NB15 network traffic dataset, utilizing in-depth exploratory data analysis, and feature selection using correlation analysis and random sampling to compare model accuracy and effectiveness. The performance and confusion matrix results indicate that the Random Forest model is the best option for identifying cyber-attacks, with a remarkable F1 score of 97.80%, accuracy of 98.63%, and low false alarm rate of 1.36%, and thus should be considered to improve IDS system security.
Correction: Patsaki et al. Benefits from Incorporating Virtual Reality in Pulmonary Rehabilitation of COPD Patients: A Systematic Review and Meta-Analysis. Adv. Respir. Med. 2023, 91, 324–336
Authors: Patsaki Irini , Avgeri Vasiliki , Rigoulia Theodora , Zekis Theodoros , Koumantakis George A. , Grammatopoulou Eirini
Published: February 2024

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Effect of Binder on Oxidation Properties of Tungsten Carbides: A Review by a Conceptual Classification Approach
Authors: Fathipour Zahra , Hadi Morteza , Maleki Mohammad Reza , Fernandes Filipe
Published: February 2024

This study presents a conceptual classification scheme to review the literature on improving the oxidation resistance of tungsten carbide by modifying the binder. The first parts of the article are dedicated to the specification of the databases, the search method, and the description of the criteria chosen to classify the articles. Then, the data collected are presented in statistical graphs according to the proposed classification scheme. The data analyzed show that most of the significant improvements in oxidation resistance are achieved with advanced production processes, especially HIP and SPS, which eliminate porosity to a very high degree. In addition, statistical studies showed that the use of new replacement binders, Ni3Al, Fe–based alloys, FeAl, and Al2O3, improved the oxidation properties in 75–100% of cases. Meanwhile, the use of high–entropy alloys (HEAs) as cermet binders may be the subject of future research for oxidation, given the recently published results of good mechanical properties.
Biodegradation of 17α-Ethinylestradiol by Strains of Aeromonas Genus Isolated from Acid Mine Drainage
Authors: Palma Tânia Luz , Costa Maria Clara
Published: February 2024

17α-ethinylestradiol (EE2), a synthetically derived analogue of endogenous estrogen, is widely employed as a hormonal contraceptive and is recognized as a highly hazardous emerging pollutant, causing acute and chronic toxic effects on both aquatic and terrestrial organisms. It has been included in the initial Water Watch List. The aim of this study was to isolate bacteria from consortia recovered from mine sediments and acid mine drainage samples, both considered extreme environments, with the ability to degrade EE2. From the most promising consortia, isolates affiliated with the Aeromonas, Rhizobium, and Paraburkholderia genera were obtained, demonstrating the capability of growing at 50 mg/L EE2. Subsequently, these isolates were tested with 9 mg/L of EE2 as the sole carbon source. Among the isolated strains, Aeromonas salmonicida MLN-TP7 exhibited the best performance, efficiently degrading EE2 (95 ± 8%) and reaching concentrations of this compound below the limits of detection within 7 and 9 days. The final metabolites obtained are in accordance with those of the TCA cycle; this may indicate EE2 mineralization. As far as is known, Aeromonas salmonicida was isolated for the first time and identified in acid mine drainage, demonstrating its capacity to degrade EE2, making it a promising candidate for bioaugmentation and suggesting its possible applicability in low pH environments.
A Comprehensive Data Pipeline for Comparing the Effects of Momentum on Sports Leagues
Authors: Noel Jordan Truman Paul , Prado da Fonseca Vinicius , Soares Amilcar
Published: February 2024

Momentum has been a consistently studied aspect of sports science for decades. Among the established literature, there has, at times, been a discrepancy between conclusions. However, if momentum is indeed an actual phenomenon, it would affect all aspects of sports, from player evaluation to pre-game prediction and betting. Therefore, using momentum-based features that quantify a team’s linear trend of play, we develop a data pipeline that uses a small sample of recent games to assess teams’ quality of play and measure the predictive power of momentum-based features versus the predictive power of more traditional frequency-based features across several leagues using several machine learning techniques. More precisely, we use our pipeline to determine the differences in the predictive power of momentum-based features and standard statistical features for the National Hockey League (NHL), National Basketball Association (NBA), and five major first-division European football leagues. Our findings show little evidence that momentum has superior predictive power in the NBA. Still, we found some instances of the effects of momentum on the NHL that produced better pre-game predictors, whereas we view a similar trend in European football/soccer. Our results indicate that momentum-based features combined with frequency-based features could improve pre-game prediction models and that, in the future, momentum should be studied more from a feature/performance indicator point-of-view and less from the view of the dependence of sequential outcomes, thus attempting to distance momentum from the binary view of winning and losing.
KDP-Net: An Efficient Semantic Segmentation Network for Emergency Landing of Unmanned Aerial Vehicles
Authors: Zhang Zhiqi , Zhang Yifan , Xiang Shao , Wei Lu
Published: February 2024

As the application of UAVs becomes more and more widespread, accidents such as accidental injuries to personnel, property damage, and loss and destruction of UAVs due to accidental UAV crashes also occur in daily use scenarios. To reduce the occurrence of such accidents, UAVs need to have the ability to autonomously choose a safe area to land in an accidental situation, and the key lies in realizing on-board real-time semantic segmentation processing. In this paper, we propose an efficient semantic segmentation method called KDP-Net for characteristics such as large feature scale changes and high real-time processing requirements during the emergency landing process. The proposed KDP module can effectively improve the accuracy and performance of the semantic segmentation backbone network; the proposed Bilateral Segmentation Network improves the extraction accuracy and processing speed of important feature categories in the training phase; and the proposed edge extraction module improves the classification accuracy of fine features. The experimental results on the UDD6 and SDD show that the processing speed of this method reaches 85.25 fps and 108.11 fps while the mIoU reaches 76.9% and 67.14%, respectively. The processing speed reaches 53.72 fps and 38.79 fps when measured on Jetson Orin, which can meet the requirements of airborne real-time segmentation for emergency landing.
Advances in Mask-Modulated Lensless Imaging
Authors: Wang Yangyundou , Duan Zhengjie
Published: February 2024

Lensless imaging allows for designing imaging systems that are free from the constraints of traditional imaging architectures. As a broadly investigated technique, mask-modulated lensless imaging encodes light signals via a mask plate integrated with the image sensor, which is more compacted, with scalability and compressive imaging abilities. Here, we review the latest advancements in mask-modulated lensless imaging, lensless image reconstruction algorithms, related techniques, and future directions and applications.
Chromatographic Analysis of the Chemical Composition of Exhaust Gas Samples from Urban Two-Wheeled Vehicles
Authors: Szymlet Natalia , Rymaniak Łukasz , Kurc Beata
Published: February 2024

The subject of the article was the chemical analysis of gasoline and exhaust gas samples taken from an urban two-wheeled vehicle. The main aim of the work was to identify chemical compounds emitted by a group of urban two-wheeled vehicles depending on the engine’s operating parameters. First, engine operating parameters and driving parameters of three urban two-wheeled vehicles were measured in real operating conditions. Based on the averaged results, engine operating points were determined for exhaust gas samples that were collected into Tedlar bags. The exhaust gas composition of individual chemical substances obtained in the chromatographic separation process were subjected to a detailed analysis relating the engine operating point with their emission rate, with each individual component being assessed in terms of its impact on human health. The obtained qualitative analysis results indicated the presence of alkenes, alkanes, aliphatic aldehydes, and aromatic and cyclic hydrocarbons (cycloalkanes) in the tested samples. The experiments provided a variety of conclusions relating to the operating parameters of a two-wheeler engine. Qualitative assessment of exhaust samples showed that a two-wheeled vehicle was characterized by the most varying composition of BTX aromatic hydrocarbons derivatives, which are particularly dangerous to human health and life. Therefore, the authors suggest that in the future, approval procedures regarding toxic emissions should be extended to include chromatographic tests. The presented results are an extension of previous studies on toxic emissions from urban two-wheeled vehicles in real operating conditions that were published in other journals.
Cross-Borehole ERT Monitoring System for CO2 Geological Storage: Laboratory Development and Validation
Authors: Jia Ninghong , Wu Chenyutong , He Chang , Lv Weifeng , Ji Zemin , Xing Lanchang
Published: February 2024

Cross-borehole electrical resistivity tomography (CHERT) technology has been implemented in field-scale CCS/CCUS (carbon capture and storage/carbon capture, utilization and storage) projects. It is highly desirable to investigate how to optimize the design of the ERT electrode arrays and corresponding working schemes for both laboratory experiments and field applications. A CHERT system was developed for laboratory experiments of CO2 geological storage applications. An optimization method was established for optimizing the structure of electrode arrays and corresponding working schemes. The developed CHERT system was calibrated systematically to determine the measurement range and accuracy of electrical impedance. Laboratory experiments were designed and implemented to validate the performance of the developed CHERT system. It has been illustrated that: (1) It is an essential step to optimize the structure of electrode arrays and corresponding working schemes of CHERT according to the real application background. The optimization method based on finite-element modelling provides an effective means for designing a field-scale CHERT system. (2) The quality of the images inverted from the CHERT data is highly dependent on the working schemes and specific modes, which is closely related to the size of the data sets used for the inversion. The AM-BN scheme is recommended due to the better uniformity of the resultant sensitivity field and application to larger borehole spacing. (3) Based on the calibration, the measurement range of the developed CHERT system can be determined as 100 Ω to 4.5 kΩ with an error limit of 1.5%. The maximum relative errors of the impedance magnitude and phase angle are 5.0% and 7.0%, respectively. Based on the test results the location of the CO2-bearing objects can be identified accurately. The shapes of the tested objects present distortion to some extent, but this can be alleviated by selecting working modes with a larger size of data set.
Use of Algae in Aquaculture: A Review
Authors: Vijayaram Srirengaraj , Ringø Einar , Ghafarifarsani Hamed , Hoseinifar Seyed Hossein , Ahani Saman , Chou Chi-Chung
Published: February 2024

The utilization of algae in aquaculture is environmentally friendly, safe, and cost-effective and can effectively substitute for fish meal and fish oil in aquatic feeds. Incorporating algae as dietary supplements leads to significant enhancements in aquatic animals’ health and also improves the aquatic ecosystem. Algae are rich sources of nutrients and serve as the foundational food source in the aquatic food chain. Currently, 40 different algae species are employed in aquaculture. Furthermore, algae contributes to elevating the overall quality of aquatic feed products. Aquaculture stands as the most vital food production sector globally; however, challenges such as infection outbreaks and aquatic environmental pollution pose significant threats to the sustainable growth of this industry. An alternative strategy for mitigating environmental issues and improving aquatic production involves the utilization of algae. The novelty in the applications of algae in aquaculture stems from their multifaceted roles and benefits, such as their capacity to improve water quality, serve as nutrient-rich feed supplements, and enhance the overall health and productivity of aquatic species. These versatile applications of algae represent a fresh and innovative approach to sustainable aquaculture practices. This review furnishes insights into the use of algae, algae extracts, or components derived from algae to enhance water quality. Additionally, it covers the utilization of algae-based feed supplements, boosting of the immune system, enhanced growth performance, and disease resistance in aquatic animals.
Numerical Analysis of Non-Newtonian Fluid Effects on the Equilibrium Position of a Suspended Particle and Relative Viscosity in Two-Dimensional Flow
Authors: Tomioka Keiya , Fukui Tomohiro
Published: February 2024

A solvent in suspension often has non-Newtonian properties. To date, in order to determine these properties, many constitutive equations have been suggested. In particular, power-law fluid, which describes both dilatant and pseudoplastic fluids, has been used in many previous studies because of its simplicity. Then, the Herschel–Bulkley model is used, which describes fluid with yield stress. In this study, we considered how a non-Newtonian solvent affected the equilibrium position of a particle and relative viscosity using the regularized lattice Boltzmann method for fluid and a two-way coupling scheme for the particle. We focused on these methods so as to evaluate the non-Newtonian effects of a solvent. The equilibrium position in Bingham fluid was closer to the wall than that in Newtonian or power-law fluid. In contrast, the tendency of relative viscosity in Bingham fluid for each position was similar to that in power-law fluid.
Identification of Novel/Rare EWSR1 Fusion Partners in Undifferentiated Mesenchymal Neoplasms
Authors: Salguero-Aranda Carmen , Di Blasi Elena , Galán Lourdes , Zaldumbide Laura , Civantos Gema , Marcilla David , de Álava Enrique , Díaz-Martín Juan
Published: February 2024

Recurrent gene fusions (GFs) in translocated sarcomas are recognized as major oncogenic drivers of the disease, as well as diagnostic markers whose identification is necessary for differential diagnosis. EWSR1 is a ‘promiscuous’ gene that can fuse with many different partner genes, defining different entities among a broad range of mesenchymal neoplasms. Molecular testing of EWSR1 translocation traditionally relies on FISH assays with break-apart probes, which are unable to identify the fusion partner. Therefore, other ancillary molecular diagnostic modalities are being increasingly adopted for accurate classification of these neoplasms. Herein, we report three cases with rare GFs involving EWSR1 in undifferentiated mesenchymal neoplasms with uncertain differential diagnoses, using targeted RNA-seq and confirming with RT-PCR and Sanger sequencing. Two GFs involved hormone nuclear receptors as 3′ partners, NR4A2 and RORB, which have not been previously reported. NR4A2 may functionally replace NR4A3, the usual 3′ partner in extraskeletal myxoid chondrosarcoma. The third GF, EWSR1::BEND2, has previously been reported in a subtype of astroblastoma and other rare entities, including a single case of a soft-tissue tumor that we discuss in this work. In conclusion, our findings indicate that the catalogue of mesenchymal neoplasm-bearing EWSR1 fusions continues to grow, underscoring the value of using molecular ancillary techniques with higher diagnostic abilities in the routine clinical setting.
Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae)
Authors: Zhou Yanan , Huang Cong , Fu Guanjun , Tang Rui , Yang Nianwan , Liu Wanxue , Qian Wanqiang , Wan Fanghao
Published: February 2024

General odorant-binding proteins (GOBPs) play a crucial role in the detection of host plant volatiles and pheromones by lepidopterans. Previous studies identified two duplications in the GOBP2 gene in Cydia pomonella. In this study, we employed qRT-PCR, protein purification, and fluorescence competitive binding assays to investigate the functions of three GOBP2 genes in C. pomonella. Our findings reveal that CpomGOBP2a and CpomGOBP2b are specifically highly expressed in antennae, while CpomGOBP2c exhibits high specific expression in wings, suggesting a potential divergence in their functions. Recombinant proteins of CpomGOBP2a, CpomGOBP2b, and CpomGOBP2c were successfully expressed and purified, enabling an in-depth exploration of their functions. Competitive binding assays with 20 host plant volatiles and the sex pheromone (codlemone) demonstrated that CpomGOBP2a exhibits strong binding to four compounds, namely butyl octanoate, ethyl (2E,4Z)-deca-2,4-dienoate (pear ester), codlemone, and geranylacetone, with corresponding dissolution constants (Ki) of 8.59993 μM, 9.14704 μM, 22.66298 μM, and 22.86923 μM, respectively. CpomGOBP2b showed specific binding to pear ester (Ki = 17.37481 μM), while CpomGOBP2c did not exhibit binding to any tested compounds. In conclusion, our results indicate a functional divergence among CpomGOBP2a, CpomGOBP2b, and CpomGOBP2c. These findings contribute valuable insights for the development of novel prevention and control technologies and enhance our understanding of the evolutionary mechanisms of olfactory genes in C. pomonella.
Can Some Anticancer Drugs Be Repurposed to Treat Amyotrophic Lateral Sclerosis? A Brief Narrative Review
Authors: Potenza Rosa Luisa , Armida Monica , Popoli Patrizia
Published: February 2024

Amyotrophic lateral sclerosis (ALS) is a rare progressive motor neuron disease that, due to its high complexity, still lacks effective treatments. Development of a new drug is a highly costly and time-consuming process, and the repositioning of approved drugs can represent an efficient strategy to provide therapeutic opportunities. This is particularly true for rare diseases, which are characterised by small patient populations and therefore attract little commercial interest. Based on the overlap between the biological background of cancer and neurodegeneration, the repurposing of antineoplastic drugs for ALS has been suggested. The objective of this narrative review was to summarise the current experimental evidence on the use of approved anticancer drugs in ALS. Specifically, anticancer drugs belonging to different classes were found to act on mechanisms involved in the ALS pathogenesis, and some of them proved to exert beneficial effects in ALS models. However, additional studies are necessary to confirm the real therapeutic potential of anticancer drugs for repositioning in ALS treatment.
From Acute to Chronic: Unraveling the Pathophysiological Mechanisms of the Progression from Acute Kidney Injury to Acute Kidney Disease to Chronic Kidney Disease
Authors: Yeh Tzu-Hsuan , Tu Kuan-Chieh , Wang Hsien-Yi , Chen Jui-Yi
Published: February 2024

This article provides a thorough overview of the biomarkers, pathophysiology, and molecular pathways involved in the transition from acute kidney injury (AKI) and acute kidney disease (AKD) to chronic kidney disease (CKD). It categorizes the biomarkers of AKI into stress, damage, and functional markers, highlighting their importance in early detection, prognosis, and clinical applications. This review also highlights the links between renal injury and the pathophysiological mechanisms underlying AKI and AKD, including renal hypoperfusion, sepsis, nephrotoxicity, and immune responses. In addition, various molecules play pivotal roles in inflammation and hypoxia, triggering maladaptive repair, mitochondrial dysfunction, immune system reactions, and the cellular senescence of renal cells. Key signaling pathways, such as Wnt/β-catenin, TGF-β/SMAD, and Hippo/YAP/TAZ, promote fibrosis and impact renal function. The renin–angiotensin–aldosterone system (RAAS) triggers a cascade leading to renal fibrosis, with aldosterone exacerbating the oxidative stress and cellular changes that promote fibrosis. The clinical evidence suggests that RAS inhibitors may protect against CKD progression, especially post-AKI, though more extensive trials are needed to confirm their full impact.
Genome-Wide Association Study and Prediction of Tassel Weight of Tropical Maize Germplasm in Multi-Parent Population
Authors: Liu Meichen , Zhang Yudong , Shaw Ranjan K. , Zhang Xingjie , Li Jinfeng , Li Linzhuo , Li Shaoxiong , Adnan Muhammad , Jiang Fuyan , Bi Yaqi , Yin Xingfu , Fan Xingming
Published: February 2024

Tassel weight (TW) is a crucial agronomic trait that significantly affects pollen supply and grain yield development in maize breeding. To improve maize yield and develop new varieties, a comprehensive understanding of the genetic mechanisms underlying tassel weight is essential. In this study, tropical maize inbred lines, namely CML312, CML373, CML444, and YML46, were selected as female parents and crossed with the elite maize inbred line Ye107, which served as the common male parent, to develop a multi-parent population comprising four F8 recombinant inbred line (RIL) subpopulations. Using 6616 high-quality single nucleotide polymorphism (SNP) markers, we conducted genome-wide association analysis (GWAS) and genomic selection (GS) on 642 F8 RILs in four subpopulations across three different environments. Through GWAS, we identified 16 SNPs that were significantly associated with TW, encompassing two stable loci expressed across multiple environments. Furthermore, within the candidate regions of these SNPs, we discovered four novel candidate genes related to TW, namely Zm00001d044362, Zm00001d011048, Zm00001d011049, and Zm00001d031173 distributed on chromosomes 1, 3, and 8, which have not been previously reported. These genes are involved in processes such as signal transduction, growth and development, protein splicing, and pollen development, all of which play crucial roles in inflorescence meristem development, directly affecting TW. The co-localized SNP, S8_137379725, on chromosome 8 was situated within a 16.569 kb long terminal repeat retrotransposon (LTR-RT), located 22.819 kb upstream and 26.428 kb downstream of the candidate genes (Zm00001d011048 and Zm00001d011049). When comparing three distinct GS models, the BayesB model demonstrated the highest accuracy in predicting TW. This study establishes the theoretical foundation for future research into the genetic mechanisms underlying maize TW and the efficient breeding of high-yielding varieties with desired tassel weight through GS.
Historical and Molecular Perspectives on the Presence of Helicobacter pylori in Latin America: A Niche to Improve Gastric Cancer Risk Assessment
Authors: González-Stegmaier Roxana , Aguila-Torres Patricia , Villarroel-Espíndola Franz
Published: February 2024

Helicobacter pylori (H. pylori) is responsible for causing chronic gastritis, which can cause peptic ulcer and premalignant lesions such as atrophic gastritis, intestinal metaplasia, and dysplasia, with the risk of developing gastric cancer. Recent data describe that H. pylori colonizes the gastric mucosa of more than 50% of the world’s population; however, this bacterium has been described as infecting the human population since its prehistory. This review focuses on the populations and subpopulations of H. pylori, differentiated by the polymorphisms present in their constitutive and virulence genes. These genes have spread and associated with different human populations, showing variability depending on their geographical distribution, and have evolved together with the human being. The predominant genotypes worldwide, Latin America and Chile, are described to understand the genetic diversity and pathogenicity of H. pylori in different populations and geographic regions. The high similarity in the sequence of virulence genes between H. pylori strains present in Peruvian and Spanish natives in Latin America suggests a European influence. The presence of cagA-positive strains and vacA s1 m1 allelic variants is observed with greater prevalence in Chilean patients with more severe gastrointestinal diseases and is associated with its geographical distribution. These findings highlight the importance of understanding the genetic diversity of H. pylori in different regions of the world for a more accurate assessment of the risk of associated diseases and their potential impact on health.
The Impact of miR-155-5p on Myotube Differentiation: Elucidating Molecular Targets in Skeletal Muscle Disorders
Authors: Lopes Letícia Oliveira , Cury Sarah Santiloni , de Moraes Diogo , Oliveira Jakeline Santos , de Oliveira Grasieli , Cabral-Marques Otavio , Fernandez Geysson Javier , Hirata Mario Hiroyuki , Wang Da-Zhi , Dal-Pai-Silva Maeli , Carvalho Robson Francisco , Freire Paula Paccielli
Published: February 2024

MicroRNAs are small regulatory molecules that control gene expression. An emerging property of muscle miRNAs is the cooperative regulation of transcriptional and epitranscriptional events controlling muscle phenotype. miR-155 has been related to muscular dystrophy and muscle cell atrophy. However, the function of miR-155 and its molecular targets in muscular dystrophies remain poorly understood. Through in silico and in vitro approaches, we identify distinct transcriptional profiles induced by miR-155-5p in muscle cells. The treated myotubes changed the expression of 359 genes (166 upregulated and 193 downregulated). We reanalyzed muscle transcriptomic data from dystrophin-deficient patients and detected overlap with gene expression patterns in miR-155-treated myotubes. Our analysis indicated that miR-155 regulates a set of transcripts, including Aldh1l, Nek2, Bub1b, Ramp3, Slc16a4, Plce1, Dync1i1, and Nr1h3. Enrichment analysis demonstrates 20 targets involved in metabolism, cell cycle regulation, muscle cell maintenance, and the immune system. Moreover, digital cytometry confirmed a significant increase in M2 macrophages, indicating miR-155’s effects on immune response in dystrophic muscles. We highlight a critical miR-155 associated with disease-related pathways in skeletal muscle disorders.
Urinary Acidification Does Not Explain the Absence of Nephrocalcinosis in a Mouse Model of Familial Hypomagnesaemia with Hypercalciuria and Nephrocalcinosis (FHHNC)
Authors: Al-Shebel Amr , Michel Geert , Breiderhoff Tilman , Müller Dominik
Published: February 2024

Patients with mutations in Cldn16 suffer from familial hypomagnesaemia with hypercalciuria and nephrocalcinosis (FHHNC) which can lead to renal insufficiency. Mice lacking claudin-16 show hypomagnesemia and hypercalciuria, but no nephrocalcinosis. Calcium oxalate and calcium phosphate are the most common insoluble calcium salts that accumulate in the kidney in the case of nephrocalcinosis, however, the formation of these salts is less favored in acidic conditions. Therefore, urine acidification has been suggested to limit the formation of calcium deposits in the kidney. Assuming that urine acidification is causative for the absence of nephrocalcinosis in the claudin-16-deficient mouse model, we aimed to alkalinize the urine of these mice by the ablation of the subunit B1 of the vesicular ATPase in addition to claudin-16. In spite of an increased urinary pH in mice lacking claudin-16 and the B1 subunit, nephrocalcinosis did not develop. Thus, urinary acidification is not the only factor preventing nephrocalcinosis in claudin-16 deficient mice.