bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Cancer spectrum and genetic characteristics of a de novo germline POLD1 p.L606M variant-induced polyposis syndrome
Authors: Zhang Ying , Wang Xiaolu , Zhu Yuning , Liang Chong , Zhao Lijun , Meng Qi , Yin Jiani C. , Shi Yuqian , Wang Fufeng , Qin Feng , Xuan Ji
Published: September 2023

Germline variations in the DNA polymerase genes, POLE and POLD1, can lead to a hereditary cancer syndrome that is characterized by frequent gastrointestinal polyposis and multiple primary malignant tumors. However, because of its rare occurrence, this disorder has not been extensively studied. In this report, we present the case of a 22-year-old female patient who had been diagnosed with gastrointestinal polyposis, breast fibroadenoma, multiple primary colorectal cancers, and glioblastoma (grade IV) within a span of 4 years. Next-generation sequencing analysis revealed a germline variant in POLD1 (c.1816C>A; p.L606M). In silico analysis using protein functional predicting software, including SIFT, Polyphen, GERP++, and CADD, further confirmed the pathogenicity of POLD1 p.L606M (classified as ACMG grade Class 4). In line with polymerase deficiency, both rectal cancer and glioblastoma tissues exhibited a high tumor mutation burden, with 16.9 muts/Mb and 347.1 muts/Mb, respectively. Interestingly, the patient has no family history of cancer, and gene examination of both parents confirms that this is a de novo germline variant. Therefore, molecular screening for POLD1 may be necessary for patients with such a cancer spectrum, regardless of their family history.
Self- and proxy-reported impaired social interaction in young adults with simple congenital heart defects
Authors: Lau-Jensen Sara Hirani , Asschenfeldt Benjamin , Evald Lars , Hjortdal Vibeke E.
Published: September 2023

Background

Simple Congenital Heart Defects such as septal defects constitute a large proportion of Congenital Heart Defects. New research has demonstrated more co-morbidities than previously thought. In particular, co-morbidities involving neurocognitive, psychiatric, and social difficulties have been described. Neurocognitive and psychiatric morbidities affect social interaction. Social interaction is important in everyday social life (education, work life, family life). In this study, we investigated social interaction through self- and proxy-answered Social Responsiveness Scale 2 (SRS-2) in young adults with simple Congenital Heart Defects and compared their social interaction profile to healthy matched controls.

Methods

We included a total of 80 patients with either atrial or ventricular septal defect (age 26.6 years) and 38 heart-healthy, age, sex, and ISCED educational matched controls (age: 25.3 years). A close relative proxy from each participant took part in the study as well. All participants answered the Social Responsiveness Scale 2 (SRS-2) (n = 225). Our primary and secondary outcomes were the SRS-2 Total score and the SRS-2 sub-scores.

Results

In the Congenital Heart Defects group, 31.3% had a Total score above 60 compared to 7.9% in the control group (p = 0.005, RR = 3.96). The participants with a septal defect had a higher Total score (52.5 vs. 45.5, p = 0.004), a higher Social Cognition sub-score (55.0 vs. 47.0, p = 0.0004), and a higher Social Motivation sub-score (50.0 vs. 45.0, p = 0.003) than the heart-healthy participants. We found no difference between the two groups regarding the sub-scores of Social Awareness and Social Communication. A multiple linear regression model showed that the variable that explained most of the variation in Total Score was having a previously diagnosed psychiatric disorder.

Conclusion

We found that young adults with atrial or ventricular septal defects have a fourfold increased risk of social interaction difficulties compared to heart-healthy peers. They have a social interaction profile, with difficulties in social cognition and social motivation, and preserved social awareness and social communication. Psychiatric morbidity explained most of the variation in social interaction problems. As social difficulties and psychiatric morbidities are intertwined, social interaction difficulties could be an indication of already underlying psychiatric morbidities or a risk factor for future psychiatric morbidity.
CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis
Authors: Jamal Muhammad , Lei Yufei , He Hengjing , Zeng Xingruo , Bangash Hina Iqbal , Xiao Di , Shao Liang , Zhou Fuling , Zhang Quiping
Published: September 2023

Introduction: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy of the lymphoid progenitor cells, contributing to ∼ 20% of the total ALL cases, with a higher prevalence in adults than children. Despite the important role of human T-ALL cell lines in understanding the pathobiology of the disease, a detailed comparison of the tumorigenic potentials of two commonly used T-ALL cell lines, MOLT4 and JURKAT cells, is still lacking.

Methodology: In the present study, NOD-PrkdcscidIL2rgdull (NTG) mice were intravenously injected with MOLT4, JURKAT cells, and PBS as a control. The leukemiac cell homing/infiltration into the bone marrow, blood, liver and spleen was investigated for bioluminescence imaging, flow cytometry, and immunohistochemistry staining. Gene expression profiling of the two cell lines was performed via RNA-seq to identify the differentially expressed genes (DEGs). CCR9 identified as a DEG, was further screened for its role in invasion and metastasis in both cell lines in vitro. Moreover, a JURKAT cell line with overexpressed CCR9 (Jurkat-OeCCR9) was investigated for T-ALL formation in the NTG mice as compared to the GFP control. Jurkat-OeCCR9 cells were then subjected to transcriptome analysis to identify the genes and pathways associated with the upregulation of CCR9 leading to enhanced tumirogenesis. The DEGs of the CCR9-associated upregulation were validated both at mRNA and protein levels. Simvastatin was used to assess the effect of cholesterol biosynthesis inhibition on the aggressiveness of T-ALL cells.

Results: Comparison of the leukemogenic potentials of the two T-ALL cell lines showed the relatively higher leukemogenic potential of MOLT4 cells, characterized by their enhanced tissue infiltration in NOD-PrkdcscidIL2rgdull (NTG) mice. Transcriptmoe analysis of the two cell lines revealed numerous DEGs, including CCR9, enriched in vital signaling pathways associated with growth and proliferation. Notably, the upregulation of CCR9 also promoted the tissue infiltration of JURKAT cells in vitro and in NTG mice. Transcriptome analysis revealed that CCR9 overexpression facilitated cholesterol production by upregulating the expression of the transcriptional factor SREBF2, and the downstream genes: MSMO1, MVD, HMGCS1, and HMGCR, which was then corroborated at the protein levels. Notably, simvastatin treatment reduced the migration of the CCR9-overexpressing JURKAT cells, suggesting the importance of cholesterol in T-ALL progression.

Conclusions: This study highlights the distinct tumorigenic potentials of two T-ALL cell lines and reveals CCR9-regulated enhanced cholesterol biosynthesis in T-ALL.
Multi-biological functions of intermedin in diseases
Authors: Yang Zhi , Li Hongchun , Wu Pengfei , Li Qingyan , Yu ChunYan , Wang Denian , Li Weimin
Published: September 2023

Intermedin (IMD) is a member of the calcitonin gene-related peptide (CGRP)/calcitonin (CT) superfamily, and it is expressed extensively throughout the body. The typical receptors for IMD are complexes composed of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein (RAMP), which leads to a biased activation towards Gαs. As a diagnostic and prognostic biomarker, IMD regulates the initiation and metastasis of multiple tumors. Additionally, IMD functions as a proangiogenic factor that can restrain excessive vascular budding and facilitate the expansion of blood vessel lumen, ultimately resulting in the fusion of blood vessels. IMD has protective roles in various diseases, including ischemia-reperfusion injury, metabolic disease, cardiovascular diseases and inflammatory diseases. This review systematically elucidates IMD’s expression, structure, related receptors and signal pathway, as well as its comprehensive functions in the context of acute kidney injury, obesity, diabetes, heart failure and sepsis. However, the precise formation process of IMD short peptides in vivo and their downstream signaling pathway have not been fully elucidated yet. Further in-depth studies are need to translate IMD research into clinical applications.
Identification of key genes responsible for green and white colored spathes in Anthurium andraeanum (Hort.)
Authors: Li Jieni , Tan Quanya , Yi Maosheng , Yu Zhengnan , Xia Qing , Zheng Lu , Chen Jianjun , Zhou Xiaoyun , Zhang Xiang-Qian , Guo He-Rong
Published: September 2023

Modern anthuriums, Anthurium andraeanum (Hort.) are among the most popular flowering plants and widely used for interior decoration. Their popularity is largely attributed to the exotic spathes with different colors. Previous studies have reported color development in red spathe cultivars, but limited information is available on key genes regulating white and green colored spathes. This study analyzed anthocyanin, chlorophyll, and carotenoid contents as well as transcript differences in spathes of eight cultivars that differed in spathe colors ranging from red to white and green. Results showed that increased expression of a transcription factor AaMYB2 was associated with elevated levels of anthocyanin in spathes, but decreased expression of AaMYB2 and increased expression of AaLAR (leucoanthocyanidin reductase) and AaANR (anthocyanidin reductase) were accompanied with the accumulation of colorless proanthocyanidin, thus the white spathe. As to the green colored spathe, chlorophyll content in the green spathe cultivar was substantially higher than the other cultivars. Correspondingly, transcripts of chlorophyll biosynthesis-related genes AaHemB (porphobilinogen synthase) and AaPor (protochlorophyllide oxidoreductase) were highly upregulated but almost undetectable in white and red spathes. The increased expression of AaHemB and AaPor was correlated with the expression of transcription factor AaMYB124. Subsequently, qRT-PCR analysis confirmed their expression levels in nine additional cultivars with red, white, and green spathes. A working model for the formation of white and green spathes was proposed. White colored spathes are likely due to the decreased expression of AaMYB2 which results in increased expression of AaLAR and AaANR, and the green spathes are attributed to AaMYB124 enhanced expression of AaHemB and AaPor. Further research is warranted to test this working model.
Dispositional mindfulness profiles in pregnant women: relationships with dyadic adjustment and symptoms of depression and anxiety
Authors: Echabe-Ecenarro Oiana , Orue Izaskun , Calvete Esther
Published: September 2023

Introduction

Pregnancy is a time of major transition that can be stressful for women. Dispositional mindfulness may protect individuals when they face stress. Recent studies have adopted a person-centered approach to examine the role of mindfulness by identifying subtypes of individuals based on their scores in five mindfulness facets. Latent profile analysis was used to identify different mindfulness profiles in a sample of pregnant women, and we explored the relationships between these profiles, depression and anxiety symptoms, and whether dyadic adjustment mediated these relationships.

Method

A total of 535 women aged 18–45 years in their 26th week of pregnancy completed questionnaires regarding mindfulness, dyadic satisfaction and cohesion, and depression and anxiety symptoms.

Results

Three profiles were identified: (1) low mindfulness (53.8%), (2) moderate mindfulness (34.3%), and (3) non-judgmentally aware (11.9%). The most adaptive profile was the non-judgmentally aware profile. Compared to the low mindfulness profile, the non-judgmentally aware profile and the moderate mindfulness profile were related to fewer symptoms of depression and anxiety, and these relationships were partly mediated by dyadic satisfaction.

Discussion

These results suggest that analyzing each pregnant woman’s mindfulness profile can improve the prevention of and interventions for anxiety and depression.
Testing a mediation model of teacher caring, grit, and student wellbeing in English as a foreign language students
Authors: Zhou Guanbing
Published: September 2023

Introduction

This study delves into the influence of perceived teacher caring on the wellbeing of Chinese English as a Foreign Language (EFL) students, with a specific focus on the potential mediating effect of grit. With a sample size of 748 EFL students hailing from diverse Chinese universities, we aimed to shed light on the connections between perceived teacher caring, student wellbeing, and the mediating factor of grit.

Methods

To address our research questions, we collected data through self-report questionnaires that gauged perceived teacher caring, student wellbeing, and levels of grit. By utilizing structural equation modeling, we were able to rigorously assess both the direct and indirect impacts of teacher caring on student wellbeing.

Results

Our analysis uncovered a noteworthy and positive correlation between perceived teacher caring and student wellbeing. Furthermore, our findings indicated that grit plays a significant mediating role in this relationship. This suggests that students who exhibit higher levels of grit are more inclined to experience heightened levels of wellbeing.

Discussion

The outcomes of this study underscore the significance of nurturing positive teacher-student relationships within the EFL context. The identification of grit as a mediator emphasizes its role in fostering enhanced student wellbeing. These findings collectively emphasize the intertwined nature of teacher caring, grit, and student wellbeing. Educators should consider these insights for their teaching practices, while researchers can use this study as a foundation for further investigations in this domain.
Low thalamic activity during a digit-symbol substitution task is associated with symptoms of subjective cognitive decline
Authors: Mizuno Akiko , Karim Helmet Talib , Ly Maria J. , Lopresti Brian J. , Cohen Ann D. , Ali Areej A. , Mathis Chester A. , Klunk William E. , Aizenstein Howard J. , Snitz Beth E.
Published: September 2023

Introduction

Subjective cognitive decline (SCD) may represent the earliest preclinical stage of Alzheimer's Disease (AD) for some older adults. However, the underlying neurobiology of SCD is not completely understood. Since executive function may be affected earlier than memory function in the progression of AD, we aimed to characterize SCD symptoms in terms of fMRI brain activity during the computerized digit-symbol substitution task (DSST), an executive function task. We also explored associations of DSST task performance with brain activation, SCD severity, and amyloid-ß (Aß) load.

Methods

We analyzed data from 63 cognitively normal older individuals (mean age 73.6 ± 7.2) with varying degree of SCD symptoms. Participants completed a computerized version of DSST in the MR scanner and a Pittsburgh Compound-B (PiB)-PET scan to measure global cerebral Aß load.

Results

A voxel-wise analysis revealed that greater SCD severity was associated with lower dorsomedial thalamus activation. While task performance was not associated with brain activation nor Aß load, slower reaction time was associated with greater SCD severity.

Discussion

The observed lower dorsomedial thalamus activation may reflect declining familiarity-based working memory and the trans-thalamic executive function pathway in SCD. SCD symptoms may reflect altered neural function and subtle decline of executive function, while Aß load may have an indirect impact on neural function and performance. Self-perceived cognitive decline may serve as a psychological/subjective marker reflecting subtle brain changes.
Mental health in adolescents after experiencing a flood event in Bavaria, Germany—A qualitative interview study
Authors: Schürr Alina , Elbel Johanna , Hieronimi Annika , Auer Isabel , Coenen Michaela , Böse-O'Reilly Stephan
Published: September 2023

Background

Children and adolescents are particularly vulnerable to the mental health impacts of extreme weather events (EWEs). This qualitative study aims to explore the stressful and protective factors after experiencing an EWE, such as flooding, how adolescents coped with these experiences and what mental health care they received.

Methods

Nine semi-structured interviews were conducted with young adults (18–24 years) living in Simbach am Inn, a German town affected by flooding in 2016. The interviews were analyzed using Kuckartz's qualitative content analysis.

Results

The days after the flood were described as the most stressful time. The main stressors were concern for their family, confrontation with the extent of the damage and uncertainty during the flood. In terms of protective factors, respondents cited talking about the flood, family support and helping with cleanup as the most important. Adolescents requested further mental health care in schools and not just in the immediate aftermath.

Conclusion

Future preventive and therapeutic care measures should be optimized according to protective and stressful factors. Mental health care should be offered after months and should be low-threshold. Additionally, the social environment of adolescents is essential for their mental wellbeing after an EWE and needs to be strengthened.
A study on the relationships between playfulness, physical self-efficacy, and school happiness among middle school students participating in “0th-period physical education class” in South Korea
Authors: Lee Byungchan , Joung Kihong , Jeon Wonjae
Published: September 2023

The purpose of this study is to create a scientific basis for the establishment of “0th-period physical education class” activities in schools in the future, with the expectation that the associations of morning exercise can be activated in the Korean educational community. To achieve this goal, the present study aimed to determine the relationship between the playfulness experienced during the early morning exercise of middle school students and their physical self-efficacy and education for happiness. To examine the model, questionnaires were collected from 296 middle school students located in Seoul and Gyeonggi-do, South Korea. Correlation analysis and standard multiple regression analysis were performed to analyze the data using the SPSS 21.0. The findings were as follows: First, the playfulness of the middle school “0th-period physical education class” had a significant effect on physical self-efficacy. Second, playfulness had a significant effect on education for happiness. Thirdly, physical self-efficacy was found to have a significant effect on education for happiness. Based on the results of this study, we suggest that a “0th-period physical education class” with various activities should be held during the legally required time in South Korea.
Rural–urban disparities in the incidence and treatment intensity of periodontal disease among patients with diabetes
Authors: Chen Hsueh-Fen , Lee Huey-Er , Chen I-Te , Huang Yu-Ting , Ho Pei-Shan , Karim Saleema A.
Published: September 2023

Background

Diabetes threatens population health, especially in rural areas. Diabetes and periodontal diseases have a bidirectional relationship. A persistence of rural–urban disparities in diabetes may indicate a rural–urban difference in periodontal disease among patients with diabetes; however, the evidence is lacking. This retrospective study aimed to investigate rural–urban discrepancies in the incidence and treatment intensity of periodontal disease among patients who were newly diagnosed with type 2 diabetes in the year 2010.

Methods

The present study was a retrospective cohort design, with two study samples: patients with type 2 diabetes and those who were further diagnosed with periodontal disease. The data sources included the 2010 Diabetes Mellitus Health Database at the patient level, the National Geographic Information Standardization Platform and the Department of Statistics, Ministry of Health and Welfare in Taiwan at the township level. Two dependent variables were a time-to-event outcome for periodontal disease among patients with type 2 diabetes and the treatment intensity measured for patients who were further diagnosed with periodontal disease. The key independent variables are two dummy variables, representing rural and suburban areas, with urban areas as the reference group. The Cox and Poisson regression models were applied for analyses.

Results

Of 68,365 qualified patients, 49% of them had periodontal disease within 10 years after patients were diagnosed with diabetes. Compared to urban patients with diabetes, rural (HR = 0.83, 95% CI: 0.75–0.91) and suburban patients (HR = 0.86, 95% CI: 0.83–0.89) had a lower incidence of periodontal disease. Among 33,612 patients with periodontal disease, rural patients received less treatment intensity of dental care (Rural: RR = 0.87, 95% CI: 0.83, 0.92; suburban: RR = 0.93, 95% CI: 0.92, 0.95) than urban patients.

Conclusion

Given the underutilization of dental care among rural patients with diabetes, a low incidence of periodontal disease indicates potentially undiagnosed periodontal disease, and low treatment intensity signals potentially unmet dental needs. Our findings provide a potential explanation for the persistence of rural–urban disparities in poor diabetes outcomes. Policy interventions to enhance the likelihood of identifying periodontal disease at the early stage for proper treatment would ease the burden of diabetes care and narrow rural–urban discrepancies in diabetes outcomes.
Evidence of increasing incidence of type 1 diabetes and ketoacidosis among children in the Republic of Srpska in period 2017–2022 with special focus on COVID-19 global pandemic years
Authors: Bukara-Radujkovic Gordana , Miljkovic Vesna , Ljuboja Olivera
Published: September 2023

Background and objectives

Primary focus of the research was to determine the incidence of type 1 diabetes mellitus in the period from 2017 to 2022, and whether COVID-19 had an impact on the increase in the number of newly diagnosed children with diabetes type 1 under the age of 15 in the Republic of Srpska (Bosnia and Herzegovina). In the period 2001–2016 the incidence of type 1 diabetes mellitus was 11/100,000, with an annual increasing rate of 14.2%.

Methods

Available data from pediatric endocrinology clinics, in the Republic of Srpska, on the number of newly diagnosed patients with diabetes mellitus in the period from January 1, 2017 until December 31, 2022 were used. A retrospective analysis was performed, and the capture-recapture method was used for the final assessment, and the obtained result corresponds to about 99% of the population.

Results

The total number of children in the group of 0–14 years of age diagnosed with type 1 diabetes mellitus in this period was 183, of which 96 (52.46%) were boys, and 87 (47.54%) were girls. The average age at which diabetes mellitus was diagnosed was 8.3 ± 3.9 years. Average incidence of diabetes in the period 2017–2022 was 19/100,000 (95% CI 13.1–25.0). The highest incidence was 28.7/100,000 in 2020, the first year of the global COVID-19 pandemic. Out of a total of 183 newly diagnosed cases in the period 2017–2022, 73 (39.9%) were diagnosed with ketoacidosis upon admission. The largest number of newly diagnosed children was recorded in the group of children aged 10–14 years.

Conclusion

In the last 6 years, there has been a significant increase in the incidence of type 1 diabetes mellitus in children under the age of 15. With an incidence of 19.4/100,000 in the Republic of Srpska, we entered the group of countries with high-risk for diabetes. Further steps must focus on the education of the entire society in order to recognize the symptoms of the disease in time and prevent the occurrence of ketoacidosis, which could significantly reduce the burden on health systems, especially in times of global pandemics, such as the COVID-19 pandemic.
Assessing the implementation of user-centred design standards on assistive technology for persons with visual impairments: a systematic review
Authors: Ortiz-Escobar Luisa Maria , Chavarria Mario Andres , Schönenberger Klaus , Hurst Samia , Stein Michael Ashley , Mugeere Anthony , Rivas Velarde Minerva
Published: September 2023

Despite scientific and technological advances in the field of assistive technology (AT) for people with visual impairment (VI), technological designs are frequently based on a poor understanding of the physical and social context of use, resulting in devices that are less than optimal for their intended beneficiaries. To resolve this situation, user-centred approaches in the development process of AT have been widely adopted in recent years. However, there is a lack of systematization on the application of this approach. This systematic review registered in PROSPERO (CRD42022307466), assesses the application of the ISO 9241-210 human-centred design principles in allegedly “user-centred designed” AT developments for persons with VI (see Supplementary PROSPERO Protocol). The results point to a wide variation of the depth of understanding of user needs, a poor characterization of the application of the User Centred Design (UCD) approach in the initial design phases or in the early prototyping, and a vague description of user feedback and device iteration. Among the principles set out in ISO 9241-210, the application of 5.6: “the design team includes multidisciplinary skills and perspectives” is the one for which the least evidence is found. The results show there is not enough evidence to fully assess the impact of UCD in (1) promoting innovation regarding AT products and practices, and (2) Judging if AT produced following such standards is leading to better user access, wellbeing outcomes and satisfaction. To address this gap it is necessary to, first, generate better implementation of UCD in AT development and second, to strengthen evidence regarding the implementation and outcomes of using UCD for AT. To better engage with the realities of persons with VI, we propose capacity building across development teams regarding UCD, its principles and components; better planning for UCD implementation; and cross-fertilization across engineering disciplines and social and clinical science.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=307466 PROSPERO (CRD42022307466).
RNA damage: the forgotten target of clinical compounds
Authors: Simms Nicole , Knight John R. P.
Published: September 2023

Unlike DNA, RNA can be found in every sub-cellular compartment, where it is used to impart the genetic code or perform essential catalytic activities. As a result, damage to RNA is more spatially pervasive than damage to DNA and can have profound effects on gene expression and RNA-dependent activities. The past decade has seen the pathways involved in detecting and responding to damage of specific RNAs defined. These studies largely used high concentrations of tool compounds or deletion of essential factors for the response to RNA damage to study its effects. RNA is damaged by both endogenous and exogenous agents, with the effect of exogenous agents administered as therapeutics the focus of this review. In an effort to formalise studies into clinical RNA damage biology we propose 4 types of RNA damaging drug that we divide into 2 broad classes. Class 1 drugs result from synthesis using non-canonical nucleotides, which are incorporated into RNA in place of the canonical nucleotides. This class is subdivided depending on the outcome of this misincorporation on the nascent transcript. Class 2 drugs result in covalent ligation of moieties that alter RNA structure. This class is subdivided according to the functionality of the covalent ligation—class 2a are monovalent while class 2b are divalent. We discuss the evidence for and mechanisms of RNA damage as well as highlighting the unknown factors that require further investigation to determine the molecular mechanisms of these drugs.
Chronic insomnia: are patients also suffering from PTSD symptoms?
Authors: Lardant Emma , Vialatte François , Ramdani Céline , Chauveau Frédéric , Gauriau Caroline , Storms Léna , Trousselard Marion , Léger Damien
Published: September 2023

Introduction

Insomnia is highly prevalent in the general population, and is commonly associated with somatic and psychiatric comorbidities. However, its origins remain poorly-understood. Recently, adverse childhood events (ACE), including traumatic experiences, have been found to be significantly associated with both insomnia and Post-Traumatic Stress Disorders (PTSD). Many patients with PTSD suffer from sleep disorders. However, we know much less about traumatic childhood experiences in patients with insomnia and PTSD.

Methods

Our exploratory study investigated a cohort of 43 patients (14 males, 29 females) clinically diagnosed with chronic insomnia at a sleep center, and systematically evaluated their condition using the trauma history questionnaire (THQ), and the PTSD checklist (PCL-5).

Results

Our results show that 83.72% of insomnia patients reported at least one traumatic event, while the prevalence of PTSD symptoms was 53.49%. For 11.6% of patients, insomnia began in childhood, while for 27.07% it began in adolescence. PCL-5 scores were associated with higher Insomnia Severity Index (ISI) scores, but not trauma. ISI scores were also higher for women, and positive relationships were observed between ISI scores, PCL-5 scores and the number of self-reported traumatic events among women.

Conclusions

These exploratory results highlight that the relationship between PTSD symptoms and insomnia could be sex-specific. They also highlight the importance of PTSD symptoms screening for patients diagnosed with chronic insomnia.
Social movements in defense of public water services: the case of Spain
Authors: Berge Jerry van den , Scheunpflug Luca , Vos Jeroen , Boelens Rutgerd
Published: September 2023

In several cities and regions in Spain there has been a fight against privatization of water supply in the past decade. Some cities have decided to re-municipalise water supply and debates about implementing the human right to water and sanitation have been held in many parts of Spain, following the success of the Right2Water European Citizens' Initiative. This paper examines how the European “Right2Water” movement influenced struggles for access to and control over water in Spain from a political ecology perspective. It explores how “Right2Water” fuelled the debate on privatization and remunicipalization of water services and what heritage it has left in Spain. We unfold relationships with and between water movements in Spain—like the Red Agua Publica—and relationships with other networks—like the indignados movement and subsequently how water protests converged with austerity protests. In different places these struggles took different shapes. By deploying five case studies (Madrid, Valladolid, Terrassa, Barcelona, and Andalucía), we look at how the human right to water and sanitation framework served as a tool for social and water justice movements. Struggles for water justice in Spain are ongoing and we seek to identify the temporarily outcomes of these struggles, and whether power balances in Spain's water services provision have shifted in the past decade.
How Are Deep-Sea Animals Getting Into Sediment Traps in Antarctica?
Authors: Kim Minkyoung , Yang Eun Jin , Kim Hyung Jeek , Kim Dongseon , Kim Tae-Wan , La Hyoung Sul , Lee SangHoon , Hwang Jeomshik
Published: September 2023

A sediment trap is a tool to collect small, sinking particles in the sea. Unexpectedly, we found deep-sea animals inside four traps placed in the Amundsen Sea, Antarctica. The animals were long, slim worms, sea urchins, and baby scallops. These animals do not swim, so how did they enter the traps? As this surprising finding happened mostly during winter, we think that ice may have helped them get into the traps. If enough of a certain kind of ice, called anchor ice, forms on an animal’s body, it can make the animal float. If the animal floats up to the sea ice on the ocean’s surface, it can attach and be transported as the ice moves around. When the anchor ice melts the animals are released, and they enter our traps as they sink to the ocean bottom. Future research is needed to know for sure if this is how the animals entered our traps!
The ability to appropriately distinguish throws for different target positions
Authors: Kusafuka Ayane , Yamamoto Rintaro , Okegawa Taishi , Kudo Kazutoshi
Published: September 2023

Repeated and accurate throwing of an object to a target position is a special human motor skill. It is particularly important to understand accuracy, which has received less attention than speed due to difficulties in measurement. Accuracy has been studied in terms of reducing errors against a single target, but also in terms of distinguishing appropriate throws for targets in different positions. In this study, this ability was investigated by evaluating the two-dimensional distributions of the pitch locations of 15 pitches to three target positions in university students with and without baseball experience. The center, major and minor axis length, major and minor axis ratio, slope, area, and percentage of overlapping area of the 95% confidence ellipse were compared between target positions and participants using a two-way repeated-measures analysis of variance (ANOVA). The center and area of the ellipse indicate the mean and variability of the error, respectively. The lengths of the major axes correspond to the variability of the release timing, and the minor axes correspond to the variability of the release point in space. Therefore, the ratio of the major and minor axes indicates how the variability of the pitching motion is controlled. The slope of the ellipse corresponds to the throwing arm's trajectory, and the percentage of overlap area means the ability to distinguish throws at different target positions. The result showed a main effect of participants on all indices except the center of the ellipse. This indicates that participants can generally distinguish throws by target positions regardless of their baseball experience, although participants with baseball experience may naturally reduce variability. Furthermore, participants with baseball experience demonstrated a decrease variability in release timing, which is a primary contributor to the pitch location variability, relative to the spatial variability of the pitching movements. This reduction in timing variability may be attributed to advanced motor control mechanisms.
Effects of stress conditions on plasma parameters and gene expression in the skin mucus of farmed rainbow trout (Oncorhynchus mykiss)
Authors: Fiordelmondo Elisa , Magi Gian Enrico , Friedl Adina , El-Matbouli Mansour , Roncarati Alessandra , Saleh Mona
Published: September 2023

The aim of this study was to investigate the physiological response of rainbow trout (Oncorhynchus mykiss) before slaughtering in the last phase of farming analyzing skin mucus and plasma. Two groups of rainbow trout were considered: Group UN (“unstressed”), represented by fish randomly captured from raceways, in the last phase of a standard fattening cycle; Group S (“stressed”), collected at the end of the pre-slaughtering tank, soon after slaughtering. The fish skin mucus was swabbed from head to tail using a sterile plastic spatula and the blood was collected through an endocardial puncture. qRT-PCR was used to study the gene expression in skin mucus. The mRNA expression levels of the IL-6 and IgD genes were higher in the S than in the Group UN. The plasma analysis showed an only a decrease in the glucose plasma levels in the Group S when compared to the Group UN. The present results indicated that the procedures adopted after slaughtering only affected changes in plasma glucose and skin mucus activity in rainbow trout suggesting that management protocol was compatible with non-stressful farming conditions.
Type of Refugee Accommodation and Health of Residents: A Cross-Sectional, Population-Based Cluster Analysis in South-West Germany
Authors: Mohsenpour Amir , Dudek Verena , Bozorgmehr Kayvan , Biddle Louise , Razum Oliver , Sauzet Odile
Published: September 2023

Objectives: Few studies have assessed whether refugees’ health is associated with accommodation characteristics. We aimed to devise a typology of refugee accommodation based on variables on the accommodation and its physical context before assessing its association with health in multivariate analyses.

Methods: We performed a cluster analysis based on a hierarchal, agglomerative clustering algorithm using Euclidean Distance and Ward’s method. We analysed accommodation clusters based on number of inhabitants, degree of housing deterioration, urbanity of location (urban/rural distinction), and remoteness (walking distance to shops, medical or administrative services). In total, we analysed health and accommodation data of 412 refugees and asylum seekers from 58 different accommodation facilities in the federal state of Baden-Württemberg in the south-west of Germany.

Results: Accommodations with a moderate occupation, lowest levels of deterioration, and a central urban location showed the best health outcomes in terms of subjective general health status, depression, and generalized anxiety disorder (GAD). Associations were strongest for GAD and weakest for depression.

Conclusion: Our findings inform policymakers on layout and location of refugee collective accommodation centres.
Numerical Simulation of Transonic Compressors with Different Turbulence Models
Authors: Yan Wenhui , Sun Zhaozheng , Zhou Junwei , Zhang Kun , Wang Jiahui , Tian Xiao , Tian Junqian
Published: September 2023

One of the most commonly used techniques in aerospace engineering is the RANS (Reynolds average Navier–Stokes) approach for calculating the transonic compressor flow field, where the accuracy of the computation is significantly affected by the turbulence model used. In this work, we use SA, SST, k-ɛ, and the PAFV turbulence model developed based on the side-biased mean fluctuations velocity and the mean strain rate tensor to numerically simulate the transonic compressor NASA Rotor 67 to evaluate the accuracy of turbulence modeling in numerical calculations of transonic compressors. The simulation results demonstrate that the four turbulence models are generally superior in the numerical computation of NASA Rotor 67, which essentially satisfies the requirements of the accuracy of engineering calculations; by comparing and analyzing the ability of the four turbulence models to predict the aerodynamic performance of transonic compressors and to capture the details of the flow inside the rotor. The errors of the Rotor 67 clogging flow rate calculated by the SA, SST, k-ɛ, and PAFV turbulence models with the experimental data are 0.9%, 0.8%, 0.7%, and 0.6%, respectively. The errors of the calculated peak efficiencies are 2.2%, 1.6%, 0.9%, and 4.9%. The SA and SST turbulence models were developed for the computational characteristics of the aerospace industry. Their computational stability is better and their outputs for Rotor 67 are comparable. The k-ɛ turbulence model calculates the pressure ratio and efficiency that are closest to the experimental data, but the computation of its details of the flow field near the wall surface is not ideal because the k-ɛ turbulence model cannot accurately capture the flow characteristics of the region of high shear stresses. The PAFV turbulence model has a better prediction of complex phenomena such as rotor internal shock wave location, shock–boundary layer interaction, etc., due to the use of a turbulent velocity scale in vector form, but the calculated rotor efficiency is small.
Fatigue Reliability Analysis of Composite Material Considering the Growth of Effective Stress and Critical Stiffness
Authors: Gao Jian-Xiong , Heng Fei , Yuan Yi-Ping , Liu Yuan-Yuan
Published: September 2023

Fatigue damage accumulation will not only cause the degradation of material performance but also lead to the growth of effective stress and critical stiffness. However, the existing fatigue reliability models usually ignore the effective stress growth and its influence on the critical stiffness of a composite material. This study considers the combined effects of performance degradation and effective stress growth, and a pair of fatigue reliability models for a composite material are presented. Firstly, the fatigue damage in a composite material is quantified by its performance degradation, and the fitting accuracy of several typical fatigue damage models is compared. Subsequently, the uncertainties of initial strength and initial stiffness are considered, and a pair of probabilistic models of residual strength and residual stiffness are proposed. The performance degradation data of Gr/PEEK [0/45/90/−45]2S laminates are utilized to verify the proposed probabilistic models. Finally, the effective stress growth mechanism and its influence on the failure threshold are elaborated, and a pair of fatigue reliability models for composite materials are developed. Moreover, the differences between the strength-based and stiffness-based reliability analysis results of composite materials are compared and discussed.
Cropping Systems and Agronomic Management Practices of Field Crops
Authors: Anastasi Umberto , Scavo Aurelio
Published: September 2023

Agriculture is facing the challenge of a transition to sustainability to meet the growing demands for food, feed, and several other renewable nonfood raw materials under a changing climatic scenario [...]
Genome-Wide Identification of Pleiotropic Drug Resistance (PDR) Transporters in Salix purpurea and Expression Analysis in Response to Various Heavy Metal Stresses
Authors: Chen Shuxin , Chen Juanjuan , Lu Zhuchou , Jia Yuhan , Yang Yuying , Zhuo Renying , Han Xiaojiao
Published: September 2023

Pleiotropic drug resistance (PDR) transporters, which are part of the ABCG subfamily of ATP-binding cassette (ABC) transporters, have been found to be involved in heavy metal tolerance. Salix species (willow) is widely regarded as a perfect candidate for phytoremediation of heavy metals because of its substantial biomass, strong tolerance, and remarkable capacity to accumulate heavy metals. However, the phylogeny and mechanisms underlying the response to heavy metals within the PDR family in willow have yet to be determined. In this study, we discovered and valuated a total of 21 PDR genes in the genome of Salix purpurea. The phylogenetic relationships of these genes were used to classify them into five major clades. The SpPDRs exhibited variations in exon-intron distribution patterns and gene lengths across different branches. Cis-acting elements linked to stress response, drought induction, low temperature, and defense response were discovered in the promoters of PDRs. Significant variations in the transcription levels of various PDR genes were observed across different tissues under heavy metal stress, with distinct heavy metals regulating different PDR members. In roots, PDR4 and PDR21 exhibited high expression levels. Meanwhile, PDR7 and PDR17 showed similar transcription patterns across all analyzed tissues. Furthermore, there was a significant and positive correlation between PDR5 and PDR16, whereas a significant and negative correlation was detected between PDR3 and PDR9, suggesting that the response of PDR members to heavy metals is complex and multifaceted. These findings will establish a vital basis for comprehending the biological functionalities of PDR genes, specifically their involvement in the regulation of willow’s tolerance to heavy metals.
Phenotype Alterations in the Cecal Ecosystem Involved in the Asymptomatic Intestinal Persistence of Paratyphoid Salmonella in Chickens
Authors: Kogut Michael H. , Fernandez Miyakawa Mariano Enrique
Published: September 2023

The gastrointestinal ecosystem involves interactions between the host, gut microbiota, and external environment. To colonize the gut of poultry, Salmonella must surmount barriers levied by the intestine including mucosal innate immune responses and microbiota-mediated niche restrictions. Accordingly, comprehending Salmonella intestinal colonization in poultry requires an understanding of how the pathogen interacts with the intestinal ecosystem. In chickens, the paratyphoid Salmonella have evolved the capacity to survive the initial immune response and persist in the avian ceca for months without triggering clinical signs. The persistence of a Salmonella infection in the avian host involves both host defenses and tolerogenic defense strategies. The initial phase of the Salmonella–gut ecosystem interaction is characteristically an innate pro-inflammatory response that controls bacterial invasion. The second phase is initiated by an expansion of the T regulatory cell population in the cecum of Salmonella-infected chickens accompanied by well-defined shifts in the enteric neuro-immunometabolic pathways that changes the local phenotype from pro-inflammatory to an anti-inflammatory environment. Thus, paratyphoid Salmonella in chickens have evolved a unique survival strategy that minimizes the inflammatory response (disease resistance) during the initial infection and then induces an immunometabolic reprogramming in the cecum that alters the host defense to disease tolerance that provides an environment conducive to drive asymptomatic carriage of the bacterial pathogen.
The Effects of 1-Deoxynojirimycin from Mulberry on Oxidative Stress and Inflammation in Laying Hens and the Direct Effects on Intestine Epithelium Cells In Vitro
Authors: Wang Mingzhu , Feng Yuan , Li Tao , Zhao Chengfeng , Barcenas Adileidys Ruiz , Serrano Boris Ramos , Qu Liang , Shen Manman , Zhao Weiguo
Published: September 2023

The intestine is highly vulnerable to various factors and has been proposed as a promising determinant for poultry health. Phytogenic or plant-derived feed additives can be used to help improve intestinal health. In this study, we aimed to investigate the effects of DNJ on the antioxidative parameters, including malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and inflammatory cytokines (IL-6, IL-1β, and TNF-α), in plasma and intestinal tissues using layers supplemented with or without the DNJ extract of mulberry leaves (DNJ-E) via the ELISA method. A total of 192 healthy Hy-Line Brown layers, aged 47 weeks old, were used to conduct a 56-day study. All hens were randomly separated into four groups as follows: a basal diet containing 0 mg/kg DNJ-E(CON), 50 mg/kg, 100 mg/kg, and 150 mg/kg DNJ-E. Furthermore, the potential mechanism by which DNJ influences intestinal function was also investigated in in vitro cultured intestinal epithelium cells (IEC) with quantification methods including the use of a cell counting kit-8 (CCK8), ELISA, qRT-PCR, and ROS detection. The results showed that CAT in plasma significantly increased following 50 mg/kg DNJ-E supplementation. Moreover, 50 mg/kg DNJ-E supplementation was associated with increases in T-SOD in the jejunum and ileum. However, there was no significant difference in inflammatory cytokines between groups in in vivo experiments. Subsequent in vitro IEC studies revealed that cell viability increased significantly following 5 µM and 10 µM DNJ treatments while decreasing significantly following 20 µM DNJ treatment. Antioxidative parameters improved at 5 µM and 10 µM DNJ concentrations. However, there were no ameliorative effects on antioxidant parameters observed under 20 µM DNJ treatment. The expression levels of Nrf2 mRNA increased significantly under DNJ treatment. DNJ treatment was associated with significant changes in the expression of genes of inflammatory cytokines. In conclusion, our study revealed that DNJ could improve oxidative stress and inflammation responses in the chicken intestine. These findings provide a theoretical reference for the development of functional feed additives that regulate intestinal health and lay the foundation for systematically revealing the mechanism of DNJ.