bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of a Barley-Based Well to Define Cationic Betaglucan to Study Mammalian Cell Toxicity Associated with Interactions with Biological Structures
Authors: Tymecka Malgorzata , Hac-Wydro Katarzyna , Obloza Magdalena , Bonarek Piotr , Kaminski Kamil
Published: July 2023

Among potential macromolecule-based pharmaceuticals, polycations seem particularly interesting due to their proven antimicrobial properties and use as vectors in gene therapy. This makes an understanding of the mechanisms of these molecules’ interaction with living structures important, so the goal of this paper was to propose and carry out experiments that will allow us to characterize these phenomena. Of particular importance is the question of toxicity of such structures to mammalian cells and, in the work presented here, two lines, normal fibroblasts 3T3-L1 and A549 lung cancer, were used to determine this. In this work, three well-defined cationic derivatives of barley-derived betaglucans obtained in a reaction with glycidyltrimethylammonium chloride (BBGGTMAC) with different degrees of cationization (50, 70, and 100% per one glucose unit) and electrostatic charge were studied. The studies address interactions of these polymers with proteins (bovine serum proteins and BSA), nucleic acids (DNA), glycosaminoglycans (heparin), and biological membranes. The results described in this study make it possible to indicate that toxicity is most strongly influenced by interactions with biological membranes and is closely related to the electrostatic charge of the macromolecule. The presentation of this observation was the goal of this publication. This paper also shows, using fluorescently labeled variants of polymers, the penetration and impact on cell structure (only for the polymer with the highest substitution binding to cell membranes is observed) by using confocal and SEM (for the polymer with the highest degree of substitution, and the appearance of additional structures on the surface of the cell membrane is observed). The labeled polymers are also tools used together with dynamic light scattering and calorimetric titration to study their interaction with other biopolymers. As for the interactions with biological membranes, lipid Langmuir monolayers as model membrane systems were used.
Sodium Alginate/Chitosan-Coated Liposomes for Oral Delivery of Hydroxy-α-Sanshool: In Vitro and In Vivo Evaluation
Authors: Tan Fengming , Li Huan , Zhang Kai , Xu Lulu , Zhang Dahan , Han Yang , Han Jing
Published: July 2023

Background: Hydroxy-α-Sanshool (HAS) possesses various pharmacological properties, such as analgesia and regulating gastrointestinal function. However, the low oral bioavailability of HAS has limited its oral delivery in clinical application. Methods and Results: To enhance its oral bioavailability, a nanocomposite delivery system based on chitosan (CH, as the polycation) and sodium alginate (SA, as the polyanion) was prepared using a layer-by-layer coating technique. The morphology, thermal behavior and Fourier transform infrared spectrum (FTIR) showed that the obtained sodium alginate/chitosan-coated HAS-loaded liposomes (SA/CH-HAS-LIP) with core-shell structures have been successfully covered with polymers. When compared with HAS-loaded liposomes (HAS-LIP), SA/CH-HAS-LIP displayed obvious pH sensitivity and a sustained-release behavior in in vitro studies, which fitted well to Weibull model. In vivo, the half-life of HAS from SA/CH-HAS-LIP remarkably extended after oral administration compared to the free drug. Additionally, it allowed a 4.6-fold and 4.2-fold increase in oral bioavailability, respectively, compared with free HAS and HAS-LIP. Conclusions: SA/CH-HAS-LIP could be a promising release vehicle for the oral delivery of HAS to increase its oral bioavailability.
Shortages of Medicines to Treat COVID-19 Symptoms during the First Wave and Fourth Wave: Analysis of Notifications Reported to Registers in Austria, Italy, and Spain
Authors: Sánchez Diana Ivonne Rodríguez , Vogler Sabine
Published: July 2023

The study aimed to investigate medicine shortages of critical relevance in the pandemic. A total of 487 active substances for the treatment of COVID-19-related symptoms and therapeutically similar medicines were reviewed as to whether or not a shortage had been notified in Austria, Italy, and Spain for February 2020, March 2020, April 2020 (first wave of the pandemic), and, in comparison, in November 2021 (fourth wave). Publicly accessible shortage registers managed by the national regulatory authorities were consulted. For 48 active substances, a shortage was notified for at least one of the study months, mostly March and April 2020. Out of these 48 active substances, 30 had been explicitly recommended as COVID-19 therapy options. A total of 71% of the active substances with notified shortage concerned medicines labeled as essential by the World Health Organization. During the first wave, Spain and Italy had higher numbers of shortage notifications for the product sample, in terms of active substances as well as medicine presentations, than Austria. In November 2021, the number of shortage notifications for the studied substances reached lower levels in Austria and Spain. The study showed an increase in shortage notifications for COVID-19-relevant medicines in the first months of the pandemic.
Association, Conformational Rearrangements and the Reverse Process of Aggregates Dissociation during Apomyoglobin Amyloid Formation
Authors: Marchenkov Victor , Balobanov Vitaly , Majorina Mariya , Ilyina Nelly , Kashparov Ivan , Glukhov Anatoly , Ryabova Natalya , Katina Natalya
Published: July 2023

Amyloid formation is linked with serious human diseases that are currently incurable. Usually, in the study of amyloid aggregation, the description of the protein’s association is in focus. Whereas the mechanism of the cross-β-structure formation, and the presence of aggregation reversibility, remain insufficiently explored. In this work, the kinetics of amyloid aggregation of apomyoglobin (ApoMb) have been studied using thioflavin fluorescence, electron microscopy, and non-denaturing electrophoresis. An analysis of the concentration dependence of the aggregation rates allows the conclusion that ApoMb amyloid formation includes the stages of conformational rearrangements in the aggregates, followed by their association and the fibril formation. The study of the mutant variants aggregation kinetics showed that the association rate is determined by the amino acids’ hydrophobicity, while the rate of conformational rearrangements is affected by the localization of the substitution. An unexpected result was the discovery that ApoMb amyloid formation is reversible, and under native-like conditions, the amyloid can dissociate, producing monomers. A consequence of the reversibility of amyloid aggregation is the presence of the monomer after aggregation completion. Since the aggregation reversibility indicates the possibility of dissociation of already formed fibrils, presented data and approaches can be useful in finding ways for amyloid diseases treatment.
Invasive Alien Species as a Potential Source of Phytopharmaceuticals: Phenolic Composition and Antimicrobial and Cytotoxic Activity of Robinia pseudoacacia L. Leaf and Flower Extracts
Authors: Uzelac Mirela , Sladonja Barbara , Šola Ivana , Dudaš Slavica , Bilić Josipa , Famuyide Ibukun M. , McGaw Lyndy J. , Eloff Jacobus N. , Mikulic-Petkovsek Maja , Poljuha Danijela
Published: July 2023

Black locust (Robinia pseudoacacia L.), an invasive tree in Europe, commonly known for its negative impact on biodiversity, is a rich source of phenolic compounds recognized in traditional medicine. Since the metabolite profile depends on the environment and climate, this study aimed to provide the first LC-MS phytochemical screening of the black locust from the Istria region (Croatia). The compounds were extracted from leaves and flowers with 70% ethanol and 80% methanol. Total phenolics (TP) and flavonoids (TF), as well as antioxidant capacity (AC) measured by ABTS (17.49–146.41 mg TE/g DW), DPPH (24.67–118.49 mg TE/g DW), and FRAP (7.38–77.53 mg TE/g DW) assays, were higher in leaf than in flower extracts. Higher TP and total non-flavonoid (TNF) values were displayed in ethanolic than in methanolic extracts. In total, 64 compounds were identified, of which flavonols (20) and hydroxycinnamic acid derivatives (15) were the most represented. Flavanols such as catechin dominated in leaf extracts, followed by flavonols, with kaempferol glucuronyl rhamnosyl hexosides as the main compound, respectively. Flower extracts had the highest share of flavones, followed by ellagitannins, with luteolin dirhamnosyl hexosides and vescalagin, respectively, being predominant. The extracts had good quorum sensing, biofilm formation prevention, and eradicating capacity. The results provided new insights into the phytochemical properties of R. pseudoacacia as the first step toward its potential pharmaceutical use.
Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (Elymus sibiricus) to Reveal Molecular Mechanisms in Response to Drought Stress
Authors: Yu Qingqing , Xiong Yi , Su Xiaoli , Xiong Yanli , Dong Zhixiao , Zhao Junming , Shu Xin , Bai Shiqie , Lei Xiong , Yan Lijun , Ma Xiao
Published: July 2023

Drought is one of the most significant limiting factors affecting plant growth and development on the Qinghai–Tibet Plateau (QTP). Mining the drought-tolerant genes of the endemic perennial grass of the QTP, Siberian wildrye (Elymus sibiricus), is of great significance to creating new drought-resistant varieties which can be used in the development of grassland livestock and restoring natural grassland projects in the QTP. To investigate the transcriptomic responsiveness of E. sibiricus to drought stress, PEG-induced short- and long-term drought stress was applied to two Siberian wildrye genotypes (drought-tolerant and drought-sensitive accessions), followed by third- and second-generation transcriptome sequencing analysis. A total of 40,708 isoforms were detected, of which 10,659 differentially expressed genes (DEGs) were common to both genotypes. There were 2107 and 2498 unique DEGs in the drought-tolerant and drought-sensitive genotypes, respectively. Additionally, 2798 and 1850 DEGs were identified in the drought-tolerant genotype only under short- and long-term conditions, respectively. DEGs numbering 1641 and 1330 were identified in the drought-sensitive genotype only under short- and long-term conditions, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that all the DEGs responding to drought stress in E. sibiricus were mainly associated with the mitogen-activated protein kinase (MAKP) signaling pathway, plant hormone signal transduction, the linoleic acid metabolism pathway, the ribosome pathway, and plant circadian rhythms. In addition, Nitrate transporter 1/Peptide transporter family protein 3.1 (NPF3.1) and Auxin/Indole-3-Acetic Acid (Aux/IAA) family protein 31(IAA31) also played an important role in helping E. sibiricus resist drought. This study used transcriptomics to investigate how E. sibiricus responds to drought stress, and may provide genetic resources and references for research into the molecular mechanisms of drought resistance in native perennial grasses and for breeding drought-tolerant varieties.
Epigenetic Regulation of Nitrogen Signaling and Adaptation in Plants
Authors: Zhang Hao , Zhang Xiaoyu , Xiao Jun
Published: July 2023

Nitrogen (N) is a crucial nutrient that plays a significant role in enhancing crop yield. Its availability, including both supply and deficiency, serves as a crucial signal for plant development. However, excessive N use in agriculture leads to environmental and economic issues. Enhancing nitrogen use efficiency (NUE) is, therefore, essential to minimize negative impacts. Prior studies have investigated the genetic factors involved in N responses and the process of low-nitrogen (LN) adaptation. In this review, we discuss recent advances in understanding how epigenetic modifications, including DNA methylation, histone modification, and small RNA, participate in the regulation of N response and LN adaptation. We highlight the importance of decoding the epigenome at various levels to accelerate the functional study of how plants respond to N availability. Understanding the epigenetic control of N signaling and adaptation can lead to new strategies to improve NUE and enhance crop productivity sustainably.
Endosperm Persistence in Arabidopsis Results in Seed Coat Fractures and Loss of Seed Longevity
Authors: Renard Joan , Bissoli Gaetano , Planes María Dolores , Gadea José , Naranjo Miguel Ángel , Serrano Ramón , Ingram Gwyneth , Bueso Eduardo
Published: July 2023

Seeds are specialized plant organs that carry, nurture, and protect plant offspring. Developmental coordination between the three genetically distinct seed tissues (the embryo, endosperm, and seed coat) is crucial for seed viability. In this study, we explore the relationship between the TFs AtHB25 and ICE1. Previous results identified ICE1 as a target gene of AtHB25. In seeds, a lack of ICE1 (ice1-2) suppresses the enhanced seed longevity and impermeability of the overexpressing mutant athb25-1D, but surprisingly, seed coat lipid polyester deposition is not affected, as shown by the double-mutant athb25-1D ice1-2 seeds. zou-4, another mutant lacking the transcriptional program for proper endosperm maturation and for which the endosperm persists, also presents a high sensitivity to seed aging. Analysis of gso1, gso2, and tws1-4 mutants revealed that a loss of embryo cuticle integrity does not underlie the seed-aging sensitivity of ice1-2 and zou-4. However, scanning electron microscopy revealed the presence of multiple fractures in the seed coats of the ice1 and zou mutants. Thus, this study highlights the importance of both seed coat composition and integrity in ensuring longevity and demonstrates that these parameters depend on multiple factors.
Phytochemical Profiling and Biological Potential of Prunus dulcis Shell Extracts
Authors: Ben Khadher Talel , Sassi-Aydi Sameh , Aydi Samir , Mars Mohamed , Bouajila Jalloul
Published: July 2023

Prunus dulcis is one of the most widely cultivated species in the world. Its fruit (almond) is rich in various nutritious and bioactive compounds that exert several beneficial effects. The aim of this study was to determine the chemical profile and evaluate the biological potential in vitro of almond shell extracts. The chemical analysis of shell extracts led to the identification of 15 compounds by HPLC-DAD, of which 11 were first detected in the almond plant. Twenty-six volatile compounds were identified by the GC-MS technique; among them, seven were firstly detected in the studied plant. For the biological activities, the extracts demonstrated moderate inhibition potential against the antioxidant, antidiabetic, and cytotoxic activities. The methanol extract at 50 µg/mL showed the highest antioxidant (45%) and antidiabetic activities (45% against alpha-glucosidase and 31% against alpha-amylase extracts), while the cyclohexane and dichloromethane at 50 µg/mL showed the highest cytotoxic activity towards Hela (32.2% with cyclohexane) and RAW 264-7 (45% with dichloromethane). Overall, these findings demonstrate the potential of almond shell extracts as a source of bioactive compounds that could be applied in the pharmaceutical and medical fields.
Impacts of Anthropogenic Activities and Climate Change on Forage Nutrition Storage in Tibetan Grasslands
Authors: Li Shaowei , Fu Gang
Published: July 2023

Uncertainties about the impacts of anthropogenic activities and climate change on forage nutrition storage of grasslands can limit the adaptive management of grasslands across the whole Tibetan Plateau. The main objective was to investigate the impacts of anthropogenic activities and climate change on the forage nutrition storage of grasslands on the Tibetan Plateau. Based on random forest models, we quantified the responses of forage nutrition storage to anthropogenic activities and climate change across the whole Tibetan grasslands from 2000 to 2020. Warming and increased precipitation did not always increase forage nutrition storage, and cooling and decreased precipitation did not always reduce forage nutrition storage. Compared to temperature and precipitation changes, radiation change had stronger contributions to potential and actual forage nutrition storage. Humankind’s activities altered the impacts of climate change on forage nutrition storage. The impacts of anthropogenic activities on forage nutrition storage increased linearly with increasing mean annual temperature and decreasing elevation but showed quadratic relationships with longitude, mean annual precipitation and radiation. The change in the impacts of humankind’s activities on forage nutrition storage was more closely related to radiation change than temperature and precipitation changes. The findings observed by this study caution that the impacts of radiation change on forage nutrition forage should be taken seriously under global change. Both climate change and humankind activities cannot always increase forage nutrition storage but may cause the degradation of forage nutrition storage.
Low-Oxygen Responses of Cut Carnation Flowers Associated with Modified Atmosphere Packaging
Authors: Nakayama Misaki , Harada Nao , Murai Ai , Ueyama Sayaka , Harada Taro
Published: July 2023

Gaseous factors affect post-harvest physiological processes in horticultural crops, including ornamental flowers. However, the molecular responses of cut flowers to the low-oxygen conditions associated with modified atmosphere packaging (MAP) have not yet been elucidated. Here, we show that storage of cut carnation flowers in a sealed polypropylene bag decreased the oxygen concentration in the bag to 3–5% and slowed flower opening. The vase life of carnation flowers after storage for seven days under MAP conditions was comparable to that without storage and was improved by the application of a commercial-quality preservative. The adenylate energy charge (AEC) was maintained at high levels in petals from florets stored under MAP conditions. This was accompanied by the upregulation of four hypoxia-related genes, among which the HYPOXIA-RESPONSIVE ETHYLENE RESPONSE FACTOR and PHYTOGLOBIN genes (DcERF19 and DcPGB1) were newly identified. These results suggest that hypoxia-responsive genes contribute to the maintenance of the energy status in carnation flowers stored under MAP conditions, making this gas-controlling technique potentially effective for maintaining cut flower quality without cooling.
Thermal, Mechanical and Tribological Properties of Gamma-Irradiated Plant-Derived Polyamide 1010
Authors: Morino Maiko , Nishitani Yosuke , Kitagawa Tatsuya , Kikutani Shinya
Published: July 2023

In this study, we investigated the influence of the gamma-irradiation dose and the addition of the cross-linking agent (triallyl isocyanurate (TAIC)) on the thermal, mechanical and tribological properties of plant-derived polyamide 1010 (PA1010). PA1010 and PA1010/TAIC were extruded using a twin screw extruder and injection molded. These specimens were then irradiated with gamma-ray in air with doses of 20 and 50 kGy. After gamma-irradiation, the specimens were heat-treated to remove the free radicals generated in the polymer. The combination of gamma-irradiation and the addition of TAIC significantly changed the crystal structures of PA1010. Glass transition temperature increased with the addition of TAIC and, in particular, with increasing gamma-irradiation dose. Moreover, PA1010/TAIC showed a rubbery plateau originating from cross-links by gamma-irradiation, which was observed in the temperature regions above the melting point in DMA measurements. Mechanical properties such as strength, modulus and hardness, and tribological properties such as frictional coefficient, specific wear rate and limiting pv (pressure p × velocity v) value of PA1010 improved with change in the internal microstructure with the gamma-irradiation and addition of TAIC.
Preparation and Characterization of Low CTE Poly(ethersulfone) Using Lignin Nano Composites as Flexible Substrates
Authors: Jeong Jieun , Kim Soochan , Yun Sangsoo , Yang Xin , Kim Young Jun
Published: July 2023

Polyethersulfone (PES) has outstanding thermal and dimensional stability. It is considered an engineering thermoplastic. However, its high coefficient of thermal expansion (CTE) hinders its use in automobiles, microelectronics, and flexible display areas. To overcome its high coefficient of thermal expansion (CTE), recent studies have focused on reducing its high CTE and improving its mechanical properties by adding nano-sized fillers or materials. The addition of nanofiller or nanofibrils to the PES matrix often has a positive effect on its mechanical and thermal properties, making it a flexible display substrate. To obtain ideal flexible substrates, we prepared polyethersulfone with lignin nanocomposite films to reduce CTE and improve the mechanical and thermal properties of PES by varying the relative ratio of PES in the lignin nanocomposite. In this study, lignin as a biodegradable nanofiller was found to show high thermal, oxidative, and hydrolytic stability with favorable mechanical properties. PES/lignin nanocomposite films were prepared by solution casting according to the content of lignin (0 to 5 wt.%). PES/lignin composite films were subjected to mechanical, thermo-mechanical, optical, and surface analyses. The results showed enhanced thermomechanical and optical properties of PES, with the potential benefits of lignin filler materials realized for the development of thermoplastic polymer blends.
Effect of the Electric Field on the Biomineralization of Collagen
Authors: Ortiz Fiorella , Díaz-Barrios Antonio , Lopez-Cabaña Zoraya E. , González Gema
Published: July 2023

Collagen/hydroxyapatite hybrids are promising biomimetic materials that can replace or temporarily substitute bone tissues. The process of biomineralization was carried out through a double diffusion system. The methodological principle consisted in applying an electric field on the incubation medium to promote the opposite migration of ions into collagen membranes to form hydroxyapatite (HA) on the collagen membrane. Two physically separated solutions were used for the incubation medium, one rich in phosphate ions and the other in calcium ions, and their effects were evaluated against the traditional mineralization in Simulated Body Fluid (SBF). Pre-polarization of the organic membranes and the effect of incubation time on the biomineralization process were also assessed by FTIR and Raman spectroscopies.Our results demonstrated that the membrane pre-polarization significantly accelerated the mineralization process on collagen. On the other side, it was found that the application of the electric field influenced the collagen structure and its interactions with the mineral phase. The increment of the mineralization degree enhanced the photoluminescence properties of the collagen/HA materials, while the conductivity and the dielectric constant were reduced. These results might provide a useful approach for future applications in manufacturing biomimetic bone-like materials.
Rigid Polyurethane Foams as Thermal Insulation Material from Novel Suberinic Acid-Based Polyols
Authors: Ivdre Aiga , Abolins Arnis , Volkovs Nikita , Vevere Laima , Paze Aigars , Makars Raimonds , Godina Daniela , Rizikovs Janis
Published: July 2023

Developing polyols from biomass sources contributes to a more circular economy by replacing petroleum-based polyols in the vast production of polyurethanes (PUR). One such potential biomass source could be leftover birch bark from which suberinic acids (SA) can be obtained. The purpose of this study was to identify the best synthesis routes for novel SA-based polyols, obtain rigid PUR foams, and evaluate their competitiveness and potential suitability as thermal insulation material. Novel polyols were synthesized from depolymerized SA by esterification with various functionality and molecular weight alcohols in several molar ratios. The moisture content, hydroxyl and acid values, and apparent viscosity were tested. Free-rise rigid PUR foams from the most suitable SA-based polyol and tall oil-based polyol were successfully prepared, reaching ~20 wt.% total renewable material content in the foam. The obtained rigid PUR foams’ morphological, mechanical, and thermal properties were investigated and compared to present foam materials, including commercial foams. The apparent density (~33 kg/m3), as well as the closed cell content (~94%), compression strength (0.25 MPa, parallel to the foaming direction), and thermal conductivity (~0.019 W/(m·K)), approved the competitiveness and potential ability of SA-based rigid PUR foam production as thermal insulation material.
Design of a 3D Amino-Functionalized Rice Husk Ash Nano-Silica/Chitosan/Alginate Composite as Support for Laccase Immobilization
Authors: Scuto Francesca Romana , Ciarlantini Clarissa , Chiappini Viviana , Pietrelli Loris , Piozzi Antonella , Girelli Anna M.
Published: July 2023

Recycling of agro-industrial waste is one of the major issues addressed in recent years aimed at obtaining products with high added value as a future alternative to traditional ones in the per-spective of a bio-based and circular economy. One of the most produced wastes is rice husk and it is particularly interesting because it is very rich in silica, a material with a high intrinsic value. In the present study, a method to extract silica from rice husk ash (RHA) and to use it as a carrier for the immobilization of laccase from Trametes versicolor was developed. The obtained mesoporous nano-silica was characterized by X-ray diffraction (XRD), ATR-FTIR spectroscopy, Scanning Elec-tron Microscopy (SEM), and Energy Dispersive X-ray spectroscopy (EDS). A nano-silica purity of about 100 % was found. Nano-silica was then introduced in a cross-linked chitosan/alginate scaffold to make it more easily recoverable after reuse. To favor laccase immobilization into the composite scaffold, functionalization of the nano-silica with (γ-aminopropyl) triethoxysilane (APTES) was performed. The APTES/RHA nano-silica/chitosan/alginate (ARCA) composite al-lowed to obtain under mild conditions (pH 7, room temperature, 1.5 h reaction time) a robust and easily reusable solid biocatalyst with 3.8 U/g of immobilized enzyme which maintained 50 % of its activity after six reuses. The biocatalytic system, tested for syringic acid bioremediation, was able to totally oxidize the contaminant in 24 h.
Equimolar Polyampholyte Hydrogel Synthesis Strategies with Adaptable Properties
Authors: Toleutay Gaukhar , Su Esra , Yelemessova Gaukhargul
Published: July 2023

Polyampholyte hydrogels exhibit great antibacterial and antifouling properties, which make them attractive for biomedical applications, such as drug delivery, wound healing, and tissue engineering. They also have potential applications in food safety, wastewater treatment, and desalination. Since they are based on ionic interactions, polyampholytes are known to require lower amounts of chemical cross-linkers as compared with traditional gels. However, the effects of both chemical and physical interactions on the material’s performance are yet to be fully understood and were examined in the present work. Here, four series of equimolar polyampholyte hydrogels were synthesized with anionic (acrylamidomethylpropane sulfonic acid sodium salt) and cationic monomers (acrylamidopropyl-trimethylammonium chloride) along with a cross-linker (N,N′-methylenebisacrylamide). The mechanical and rheological properties of the gels were characterized following changes to the initial monomer concentration and crosslinker ratios, which led to gels with different toughness, stretchability, and compressibility. The direct correlation of the cross-linking degree with the initial monomer concentration showed that the chemical crosslinker could be further reduced at a high monomer concentration of 30% by weight, which creates an inter-chain network at a minimal crosslinker concentration of 0.25%. Lastly, N′N-dimethylacrylamide was added, which resulted in an increase in the number of H-bonds in the structure, noticeably raising material performance.
Design, Synthesis, and Comparison of PLA-PEG-PLA and PEG-PLA-PEG Copolymers for Curcumin Delivery to Cancer Cells
Authors: Rostami Neda , Faridghiasi Farzaneh , Ghebleh Aida , Noei Hadi , Samadzadeh Meisam , Gomari Mohammad Mahmoudi , Tajiki Alireza , Abdouss Majid , Aminoroaya Alireza , Kumari Manisha , Heidari Reza , Uversky Vladimir N. , Smith Bryan R.
Published: July 2023

Curcumin (CUR) has potent anticancer activities, and its bioformulations, including biodegradable polymers, are increasingly able to improve CUR’s solubility, stability, and delivery to cancer cells. In this study, copolymers comprising poly (L-lactide)-poly (ethylene glycol)-poly (L-lactide) (PLA-PEG-PLA) and poly (ethylene glycol)-poly (L-lactide)-poly (ethylene glycol) (PEG-PLA-PEG) were designed and synthesized to assess and compare their CUR-delivery capacity and inhibitory potency on MCF-7 breast cancer cells. Molecular dynamics simulations and free energy analysis indicated that PLA-PEG-PLA has a higher propensity to interact with the cell membrane and more negative free energy, suggesting it is the better carrier for cell membrane penetration. To characterize the copolymer synthesis, Fourier transform-infrared (FT-IR) and proton nuclear magnetic resonance (1H-NMR) were employed, copolymer size was measured using dynamic light scattering (DLS), and their surface charge was determined by zeta potential analysis. Characterization indicated that the ring-opening polymerization (ROP) reaction was optimal for synthesizing high-quality polymers. Microspheres comprising the copolymers were then synthesized successfully. Of the two formulations, PLA-PEG-PLA experimentally exhibited better results, with an initial burst release of 17.5%, followed by a slow, constant release of the encapsulated drug up to 80%. PLA-PEG-PLA-CUR showed a significant increase in cell death in MCF-7 cancer cells (IC50 = 23.01 ± 0.85 µM) based on the MTT assay. These data were consistent with gene expression studies of Bax, Bcl2, and hTERT, which showed that PLA-PEG-PLA-CUR induced apoptosis more efficiently in these cells. Through the integration of nano-informatics and in vitro approaches, our study determined that PLA-PEG-PLA-CUR is an optimal system for delivering curcumin to inhibit cancer cells.
Influence of TPU/EVA Phase Morphology Evolution on Supercritical Carbon Dioxide Extrusion Foaming
Authors: Du Jun-Wei , Zhou Tian-Tian , Zhang Rong , Hu Sheng-Fei
Published: July 2023

Ethylene-vinyl acetate copolymer (EVA) was added at different contents to the thermoplastic polyurethane (TPU) matrix to form a non-compatible blending system, and foaming materials with high pore density were prepared using the supercritical carbon dioxide extrusion method. The influence of the phase morphology and crystal morphology of the TPU/EVA blend on its foaming behavior was studied. The results show that EVA changed the phase morphology and crystal morphology of the blends, leading to the improved melt viscosity and crystallinity of the blend system. At the same time, interfacial nucleation increases the density of cells and decreases the cell thickness and size, which is beneficial for improving the foaming properties of the blends. For the EVA content of 10% (mass fraction), the cell size is small (105.29 μm) and the cell density is the highest (3.74 × 106 cells/cm3). Based on the TPU/EVA phase morphology and crystal morphology, it is found that the sea-island structure of the blend has better foaming properties than the bicontinuous structure.
Eco-Friendly Methods for Extraction and Modification of Cellulose: An Overview
Authors: Magalhães Solange , Fernandes Catarina , Pedrosa Jorge F. S. , Alves Luís , Medronho Bruno , Ferreira Paulo J. T. , Rasteiro Maria da Graça
Published: July 2023

Cellulose is the most abundant renewable polymer on Earth and can be obtained from several different sources, such as trees, grass, or biomass residues. However, one of the issues is that not all the fractionation processes are eco-friendly and are essentially based on cooking the lignocellulose feedstock in a harsh chemical mixture, such as NaOH + Na2S, and water, to break loose fibers. In the last few years, new sustainable fractionation processes have been developed that enable the obtaining of cellulose fibers in a more eco-friendly way. As a raw material, cellulose’s use is widely known and established in many areas. Additionally, its products/derivatives are recognized to have a far better environmental impact than fossil-based materials. Examples are textiles and packaging, where forest-based fibers may contribute to renewable and biodegradable substitutes for common synthetic materials and plastics. In this review, some of the main structural characteristics and properties of cellulose, recent green extraction methods/strategies, chemical modification, and applications of cellulose derivatives are discussed.
Disturbance-Suppression Method of Direct-Driven PMSG-Based Wind Power System in Microgrids
Authors: Xu Xiuqi , Xiu Liancheng , He Jingxuan , Gong Rongxin
Published: July 2023

In order to solve the current fluctuation problem in microgrids, a suppression method called the Direct-driven Permanent Magnet Synchronous Generator (DPMSG)-based Wind Power System (WPS) based on an adaptive enhanced moving average filter algorithm is proposed. Firstly, the mathematical model of the WPS is established. On this basis, the suppression method under unbalanced conditions is derived by the instantaneous power equation to ensure the stable operation of the microgrid. In order to improve the dynamic compensation capability of the DPMSG-based WPS, an enhanced moving average filtering algorithm with frequency adaptability is proposed. The positive and negative sequence components are obtained in the dq frame by this filtering algorithm. Subsequently, the angular frequency of the microgrid is obtained according to the changing phase, which realizes the high-performance control of the WPS and avoids the complicated parameter adjustment of traditional methods. The correctness of this method is verified by the simulation results. The DPMSG-based WPS with the proposed method can improve the stability of the microgrid.
Mindful Apocalypse: Contemplative Anthropology Investigating Experiences of World-Loss in Deep Meditation
Authors: Divino Federico
Published: July 2023

This article investigates the challenge of personal crisis during deep meditation, as observed in an ethnographic inquiry into mindfulness and traditional contemplative practices. The study distinguishes between the “crisis of presence” in contemporary mindfulness practices, and the dissolution of the subject-object distinction in traditional Buddhist meditation. By analyzing Ernesto De Martino’s concepts of crisis and presence, the article highlights the significance of understanding this phenomenon in meditation rather than perceiving it negatively. The research explores the contemporary evolution of mindfulness and its detachment from original Buddhist contemplative practices, leading to an approach criticized for reinforcing neoliberal and capitalist modes of cognition. In contrast, traditional Buddhist meditation aims for the state of samādhi, where boundaries between self and the world dissolve, signifying a serene “end of the world”. The study underscores the need for mindfulness researchers to explore this aspect of meditation to derive immense benefits from comprehensive contemplative practice.
Ritual Practices and Material Culture: The Provenance and Transformation of Stūpas in Medieval China
Authors: Sun Wen
Published: July 2023

This paper examines how Chinese people perceived and accepted Buddhist stūpas in medieval China. Doctrinal and ritualistic developments can potentially contribute to the emergence of new ritual objects. Ideological connotations of stūpas witnessed a transition associated with the transformation of the stūpa cult in China. Stūpa burial became progressively accessible to ordinary clerics and laypeople who showed sympathy with Buddhism. The similarity between stūpas and tombs in terms of funerary function largely determined people’s interpretations of stūpas in the early medieval period. However, tombs cannot be the precise manifestation of stūpas in medieval China. Stūpas evolved into multidimensional meanings in medieval China. The perceptions of stūpas witnessed an ongoing process of reconstruction, which reveals how cultural transmission and transformation work throughout history.
Fast SAR Image Simulation Based on Echo Matrix Cell Algorithm Including Multiple Scattering
Authors: Wu Ke , Jin Guowang , Xiong Xin , Zhang Hongmin , Wang Limei
Published: July 2023

We present a novel fast synthetic aperture radar (SAR) image simulation method based on the echo matrix cell algorithm including multiple scattering. To improve the efficiency of SAR image simulation while ensuring the fidelity of the simulated results, we first discretized the target facets set in the SAR beams footprint into lattice targets using the range-Doppler (RD) imaging geometry model and provided the basis for simulating electromagnetic wave transmission. Based on the simulation of electromagnetic waves transmission, we used the ray tracing algorithm to calculate the multiple backscattering field including multi-polarimetric information and various material properties. Then, based on the echo matrix cell algorithm, we set the echo matrix cell as the subfield of the target backscattering field and designed the CUDA kernel function to implement a computation parallelization for SAR echo generation. All the echo matrix cells are traversed in parallel to obtain the total backscattering field of the target, reproducing the time-varying characteristic of the backscatter coefficient for each lattice target within the synthetic aperture time. The echo signal is processed using the RD imaging algorithm to obtain the simulated SAR image. Finally, we select some targets including aircraft carrier and airplane models for simulation tests. The computation efficiency is improved over 170-fold by comparing the computations of the proposed method and CPU single-thread. We also performed some qualitative and quantitative evaluations on the fidelity of the simulated SAR images. The experimental results verify the effectiveness of the proposed method.
Assessment of Spatiotemporal Patterns and the Effect of the Relationship between Meteorological Drought and Vegetation Dynamics in the Yangtze River Basin Based on Remotely Sensed Data
Authors: Dong Xiujuan , Zhou Yuke , Liang Juanzhu , Zou Dan , Wu Jiapei , Wang Jiaojiao
Published: July 2023

Global climate change and human activities have increased the frequency and severity of droughts. This has become a critical factor affecting vegetation growth and diversity, resulting in detrimental effects on agricultural production, ecosystem stability, and socioeconomic development. Therefore, assessing the response of vegetation dynamics to drought can offer valuable insights into the physiological mechanisms of terrestrial ecosystems. Here, we applied long-term datasets (2001–2020) of solar-induced chlorophyll fluorescence (SIF) and normalized difference vegetation index (NDVI) to unveil vegetation dynamics and their relationship to meteorological drought (SPEI) across different vegetation types in the Yangtze River Basin (YRB). Linear correlation analysis was conducted to determine the maximum association of SPEI with SIF and NDVI; we then compared their responses to meteorological drought. The improved partial wavelet coherence (PWC) method was utilized to quantitatively assess the influences of large-scale climate patterns and solar activity on the relationship between vegetation and meteorological drought. The results show that: (1) Droughts were frequent in the YRB from 2001 to 2020, and the summer’s dry and wet conditions exerted a notable influence on the annual climate. (2) SPEI exhibits a more significant correlation with SIF than with NDVI. (3) NDVI has a longer response time (3–6 months) to meteorological drought than SIF (1–4 months). Both SIF and NDVI respond faster in cropland and grassland but slower in evergreen broadleaf and mixed forests. (4) There exists a significant positive correlation between vegetation and meteorological drought during the 4–16 months period. The teleconnection factors of Pacific Decadal Oscillation (PDO), El Niño Southern Oscillation (ENSO), and sunspots are crucial drivers that affect the interaction between meteorological drought and vegetation, with sunspots having the most significant impact. Generally, our study indicates that drought is an essential environmental stressor that disrupts vegetation growth over the YRB. Additionally, SIF demonstrates great potential in monitoring vegetation response to drought. These findings will be meaningful for drought prevention and ecosystem conservation planning in the YRB.
Structure of the High-Latitude Noon Ionosphere of the Southern Hemisphere
Authors: Karpachev Alexander
Published: July 2023

The structure of the winter noon ionosphere of the southern hemisphere was studied. This structure includes the dayside cusp, associated high-latitude ionospheric trough (HLT), main ionospheric trough (MIT), electron density (Ne) peak at latitudes about 70°, mid-latitude ring ionospheric trough (RIT), and low-latitude quasi-trough. Data from the CHAMP satellite in the southern hemisphere for quiet geomagnetic conditions under high solar activity were selected for analysis. The DMSP satellite data and a model of auroral diffuse precipitation were also used. This model represents two zones of auroral diffuse precipitation on the equatorward and poleward edges of the auroral oval. It is shown that the situation in the winter noon ionosphere of the southern hemisphere depends cardinally on longitude. At sunlit longitudes, only the HLT is observed, and MIT is formed in the shadow region. At intermediate longitudes, both troughs can be observed and, therefore, there is a problem of their separation. The positions of all structures of the ionosphere depend on the longitude; in particular, the positions of the daytime MIT are changed by 6°−7°. At latitudes of the dayside cusp, both the peak and the minimum of Ne can be observed. A high and narrow peak of Ne is regularly recorded in the CHAMP data at latitudes of the equatorward zone of diffuse precipitation (68°−72°). In the shadow region, this peak forms the MIT poleward wall, and at sunlit longitudes a quasi-trough equatorward of this peak is sometimes observed. The RIT is rarely formed during the day, only at the American and Atlantic longitudes.
Comparison of Spring Wind Gusts in the Eastern Part of the Tibetan Plateau and along the Coast: The Role of Turbulence
Authors: Zhou Xingxu , Zhang Chao , Li Yunying , Zhang Zhiwei
Published: July 2023

Wind gusts are sudden, brief increases in wind speed that have important implications for wind power generation, building design, aviation and marine safety. However, wind gusts in the Plateau and coastal plain are very different. In this paper, the gust characteristics are explored and compared at two sites in the same latitude—Xining, a city in the eastern Tibetan Plateau, and Qingdao, a city in the coast in China—using Doppler lidar data. The results indicate that the wind gusts in Xining are more intense and occur at a higher height than those in Qingdao. Though mean winds and turbulence significantly influence gusts, the turbulence intensity is responsible for the differences in gust, and high turbulence in the eastern part of the Tibetan Plateau is inferred. These results provide observational evidence for wind gusts over the complex terrain of the Tibetan Plateau and are useful for studying their impact on important aspects, such as flight safety.
MISPEL: A Multi-Crop Spectral Library for Statistical Crop Trait Retrieval and Agricultural Monitoring
Authors: Borrmann Peter , Brandt Patric , Gerighausen Heike
Published: July 2023

Spatiotemporally accurate estimates of crop traits are essential for both scientific modeling and practical decision making in sustainable agricultural management. Besides efficient and concise methods to derive these traits, site- and crop-specific reference data are needed to develop and validate retrieval methods. To address this shortcoming, this study first includes the establishment of ’MISPEL’, a comprehensive spectral library (SpecLib) containing hyperspectral measurements and reference data for six key traits of ten widely grown crops. Secondly, crop-specific statistical leaf area index (LAI) models for winter wheat are developed based on a hyperspectral (MISPELFR) and a simulated Sentinel-2 (MISPELS2) SpecLib applying four nonparametric methods. Finally, an independent Sentinel-2 model evaluation at the DEMMIN test site in Germany is conducted, including a comparison with the commonly used SNAP-LAI product. To date, MISPEL comprises a set of 1411 spectra of ten crops and more than 6800 associated reference measurements. Cross-validations of winter wheat LAI models revealed that Elastic-net generalized linear model (GLMNET) and Gaussian process (GP) regressions outperformed partial least squares (PLS) and random forest (RF) regressions, showing RSQ values up to 0.86 and a minimal NRMSE of 0.21 using MISPELFR. GLMNET and GP models based on MISPELS2 further outperformed SNAP-based LAI estimates derived for the external validation site. Thus, it is concluded that the presented SpecLib ’MISPEL’ and applied methodology have a very high potential for deriving diverse crop traits of multiple crops in view of most recent and future multi-, super-, and hyperspectral satellite missions.
Cartography of the Solar System: Remote Sensing beyond Earth
Authors: van Gasselt Stephan , Naß Andrea
Published: July 2023

Cartography is traditionally associated with map making and the visualization of spatial information [...]
Improving Diagnostics with Deep Forest Applied to Electronic Health Records
Authors: Khodadadi Atieh , Ghanbari Bousejin Nima , Molaei Soheila , Kumar Chauhan Vinod , Zhu Tingting , Clifton David A.
Published: July 2023

An electronic health record (EHR) is a vital high-dimensional part of medical concepts. Discovering implicit correlations in the information of this data set and the research and informative aspects can improve the treatment and management process. The challenge of concern is the data sources’ limitations in finding a stable model to relate medical concepts and use these existing connections. This paper presents Patient Forest, a novel end-to-end approach for learning patient representations from tree-structured data for readmission and mortality prediction tasks. By leveraging statistical features, the proposed model is able to provide an accurate and reliable classifier for predicting readmission and mortality. Experiments on MIMIC-III and eICU datasets demonstrate Patient Forest outperforms existing machine learning models, especially when the training data are limited. Additionally, a qualitative evaluation of Patient Forest is conducted by visualising the learnt representations in 2D space using the t-SNE, which further confirms the effectiveness of the proposed model in learning EHR representations.