bluuebunny/crossref_metadata_2025 huggingface dataset:
Intra-hypothalamic circuit orchestrates β-endorphin release following coital ejaculation in male mice
Authors: Zha Xi , Jiao Zhuo-Lei , Li Shuai-Shuai , Liu Xiao-Yao , Li Xing-Yu , Sun Yi-Zhuo , Ding Xiao-Jing , Gao Meng-Tong , Gao Shu-Chen , Chen Ai-Xiao , Lin Jun-Kai , Zhang Wen , Cao Xuan-Zi , Zhang Yan-Li , Yang Rong-Rong , Xu Chun , Xu Xiao-Hong
Published: September 2024

Survey-based evidence suggests that men experience a distinct post-ejaculation affective state1,2, marked by intense pleasure sometimes compared to the euphoric rush from intravenous injection of opioid drugs such as heroin3. However, the intrinsic neural circuit mechanisms underlying the ejaculation-triggered affective state remain unclear. Here, we discovered thatCalbindin1-expressing (Calb1+) neurons in the preoptic area (POA) of the hypothalamus, an evolutionarily conserved regulatory region for male mating behavior4, are specifically activated during ejaculation in male mice. Inhibiting POACalb1+neurons prolongs mating and delays ejaculation. Importantly, POACalb1+neurons transmit the ejaculation signal and activate proopiomelanocortin-expressing (Pomc+) neurons in the arcuate nucleus of the hypothalamus, which show robust and sustained activity lasting for tens of seconds, specifically upon ejaculation. This activity is accompanied by elevated levels of β-endorphins5, opioid peptides secreted byPomc+neurons, post-ejaculation in male mice. Optogenetic activation ofPomc+neurons increases β-endorphins levels and conditioned placed preference, similar to ejaculation. Conversely, intracerebroventricular (i.c.v.) infusion of drugs blockingPomcneuropeptides signaling eliminates ejaculation-conditioned place preference. Collectively, these results elucidate an intra-hypothalamic circuit from POACalb1+neurons to arcuatePomc+neurons that coordinate β-endorphin release with ejaculation, shedding light on the neurobiological basis of the post-ejaculation affective state.
Basal activation of astrocytic Nrf2 in neuronal culture media: challenges and implications for neuron-astrocyte modelling
Authors: Elsharkasi M.M.O , Villani B , Wells G , Kerr F
Published: September 2024

As a gatekeeper of anti-oxidant and anti-inflammatory cell protection, the transcription factor Nrf2 is a promising therapeutic target for several neurodegenerative diseases, leading to the development of Nrf2 activators targeting Keap1-dependent and independent regulatory mechanisms. Astrocytes play a crucial role in regulating neuronal physiology in health and disease, including Nrf2 neuroprotective responses. As neurons require specific conditions for their differentiation and maintenance, most 2D and 3D co-culture systems use medias that are compatible with neuronal differentiation and function, but also ensure astrocyte survival. Few studies, however, assess the molecular adaptations of astrocytes to changes from astrocyte maintenance medias alone, and their subsequent effects on neurons which may represent technical rather than physiological responses.

Our findings show that while Nrf2 can be effectively activated by the Keap1-Nrf2 protein-protein interaction disruptor 18e, and classical Nrf2 activators DMF and CDDO-Me, in human primary cortical astrocyte monocultures, their efficacy is lost in LUHMES neuron-astrocyte co-cultures. Further investigation revealed that the Advanced DMEM/F12-based LUHMES differentiation media maximally induced basal Nrf2 activity in astrocytes alone, compared to astrocyte maintenance media, thus preventing pharmacological activation. Although Neurobasal slightly activated basal Nrf2, this was not significant suggesting that this media has less impact on astrocytic Nrf2 activity relative to Advanced DMEM/F12.

As Nrf2 is a key regulator of oxidative damage and neuroinflammation, modelling these common features of neurodegenerative diseases may be confounded by environments that maximally activate basal Nrf2. Our findings thus suggest caution in media selection for neuron-astrocyte co-culture in disease modelling and therapeutic Nrf2 activator discovery.
Substrate-specific regulation of mTORC1 activity by G protein-coupled receptors
Authors: Atkinson Samuel J. , Ritchie William Vincent , Thompson Kyle , Thompson Dawn , Hislop James N. , Hatakeyama Riko
Published: September 2024

The mammalian/mechanistic Target of Rapamycin Complex 1 (mTORC1) kinase controls cell growth in response to various external stimuli. mTORC1 has a myriad of substrates, and the activation of distinct downstream pathways has important physiological outcomes. Emerging evidence suggests that mTORC1 can respond to upstream signals in a nuanced manner, differentially regulating individual substrates and downstream biological processes. The nature of signals that determine the signaling selectivity of mTORC1 is not fully understood. Here, we studied mTORC1 regulation by G protein-coupled receptors (GPCRs). We found that muscarinic acetylcholine receptor M5, formyl peptide receptor 2, and beta-2 adrenergic receptor, which are coupled to distinct effector G proteins, all trigger substrate-specific activation of mTORC1. Remarkably, phosphorylation of the TFEB transcription factor, a non-canonical mTORC1 substrate that controls lysosome biogenesis, responded to GPCRs differently, sometimes even oppositely, compared to canonical mTORC1 substrates such as S6K1 and 4EBP1. This study highlights the need to re-evaluate the effects of GPCRs on mTORC1 by parallelly monitoring individual substrates, an important consideration to be made when assessing GPCR ligands as therapeutic tools to manipulate the mTORC1 pathway.
Single Cell Profiling in theSox10Dom/+Hirschsprung Mouse ImplicatesHoxa6in Enteric Neuron Lineage Allocation
Authors: Avila Justin A. , Benthal Joseph T. , Schafer Jenny C. , Southard-Smith E. Michelle
Published: September 2024

Background & Aims

Enteric nervous system (ENS) development requires migration, proliferation, and appropriate neuronal diversification from progenitors to enable normal gastrointestinal (GI) motility.Sox10deficit causes aganglionosis, modeling Hirschsprung disease, and disrupts ratios of postnatal enteric neurons in proximal ganglionated bowel. HowSox10deficiency alters ratios of enteric neuron subtypes is unclear.Sox10’sprominent expression in enteric neural crest-derived progenitors (ENCP) and lack of this gene in enteric neurons led us to examineSox10Domeffects ENS progenitors and early differentiating enteric neurons.

Methods

ENS progenitors, developing neurons, and enteric glia were isolated fromSox10+/+andSox10Dom/+littermates for single-cell RNA sequencing (scRNA-seq). scRNA-seq data was processed to identify cell type-specific markers, differentially expressed genes, cell fate trajectories, and gene regulatory network activity between genotypes. Hybridization chain reaction (HCR) validated expression changes detected in scRNA-seq.

Results

scRNA-seq profiles revealed three neuronal lineages emerging from cycling progenitors via two transition pathways accompanied by elevated activity ofHoxgene regulatory networks (GRN) as progenitors transition to neuronal fates.Sox10Dom/+scRNA-seq profiles exhibited a novel progenitor cluster, decreased abundance of cells in transitional states, and shifts in cell distributions between two neuronal trajectories.Hoxa6was differentially expressed in the neuronal lineages impacted inSox10Dom/+mutants and HCR identified alteredHoxa6expression in early developing neurons ofSox10Dom/+ENS.

Conclusions

Sox10Dom/+mutation shifts enteric neuron types by altering neuronal trajectories during early ENS lineage segregation. Multiple neurogenic transcription factors are reduced inSox10Dom/+scRNA-seq profiles including multipleHoxgenes. This is the first report that implicatesHoxgenes in lineage diversification of enteric neurons.
Tomato spotted wilt virus facilitates non-vector spider mite species (Tetranychus urticaeandTetranychus evansi) on whole tomato plants
Authors: Gupta Vandana , Grosjean Sarah , Moreau Aloyse , Carbonell David , Duncan Alison B.
Published: September 2024

Coinfections where hosts harbour more than one parasite species are common in nature. Facilitation among parasites enabling them to better exploit host resources is widespread with direct consequences on their life-history. Plant viruses can facilitate their vectors to increase their transmission, but equivalent studies in virus-non vectors are lacking. Here we study facilitation of two (non-vector) species ofTetranychusspider mites,Tetranychus urticaeandT. evansi, by a plant virus, tomato spotted wilt virus (TSWV), in coinfections on a host tomato plant,Solanum lycopersicum. We compared the effect of different strains of TSWV on life-history traits ofTetranychusmites on cut leaves and whole plants of different tomato varieties. TSWV facilitated both species of spider mite on two different tomato varieties on whole plants, with more offspring of both species becoming adults. In contrast, on cut leaves, facilitation ofT. urticaewas much more variable depending on the experiment, viral strain and tomato variety. We attribute this to the non-homogeneous spread of virus throughout the host plant. Virus is first transported to the top leaves, and not middle leaves which we had used for the experiment. Indeed, if facilitation is indirect via the host immune system or resource based via the release of free-amino acids then this may be less efficient on cut plant parts. These results highlight that facilitation among parasites may increase parasite fitness and at the same time levels of virulence experienced by the host.
Role of copper during microglial inflammation
Authors: Craciun Laura , Muroy Sandra E. , Saijo Kaoru
Published: September 2024

Copper plays crucial roles in various physiological functions of the nervous and immune systems. Dysregulation of copper homeostasis is linked to several diseases, including neurodegenerative diseases. Since dysfunctional microglial immunity can contribute to such diseases, we investigated the role of copper in microglial immunity. We found that both increased and decreased copper levels induced by chemical treatments suppresses lipopolysaccharide (LPS)-mediated inflammation in microglial cells, as determined by RT-qPCR analysis. RNA sequencing (RNA-seq) analysis confirmed that increased copper level reduces the inflammatory response to LPS; however, it also showed that decreased copper level affects genes involved in cell proliferation, transcription, and autophagosome regulation. These findings suggest that copper is vital for maintaining normal immune function in microglia, and both copper excess and deficiency can disrupt microglial immunity.
Uncovering the mechanistic basis of intracellular Raf inhibitor sensitivity reveals synergistic cotreatment strategies
Authors: Stoddard Ethan G. , Fang Linglan , Zhong Yuhao , Potter Zachary E. , Brush Daniel S. , Simon Jessica J. , Golkowski Martin , Maly Dustin J.
Published: September 2024

Raf kinases are crucial effectors in the Ras-Raf-Mek-Erk signaling pathway, making them important targets for the development of cancer therapeutics. This study investigates the variable potency of DFG-out-stabilizing Raf inhibitors in mutant KRas-expressing cell lines. We demonstrate that inhibitor potency correlates with basal Raf activity, with more active Raf being more susceptible to inhibition. We further show that DFG-out-stabilizing inhibitors disrupt high-affinity Raf-Mek interactions, promoting the formation of inhibited Raf dimers. Furthermore, we identify cobimetinib as a Mek inhibitor that uniquely sensitizes Raf to DFG-out inhibitors by disrupting autoinhibited Raf-Mek complexes. Building on this insight, we developed cobimetinib analogs with enhanced sensitization properties. Our findings provide a mechanistic framework for understanding the cellular determinants of DFG-out-stabilizing inhibitor sensitivity and offer strategies for optimizing synergistic Raf-Mek inhibitor combinations.
Speed modulations in grid cell information geometry
Authors: Ye Zeyuan , Wessel Ralf
Published: September 2024

Grid cells, known for their hexagonal spatial firing patterns, are widely regarded as essential to the brain’s internal representation of the external space. Maintaining an accurate internal spatial representation is challenging when an animal is running at high speeds, as its self-location constantly changes. Previous studies of speed modulation of grid cells focused on individual or pairs of grid cells, yet neurons represent information via collective population activity. Population noise covariance can have significant impact on information coding that is impossible to infer from individual neuron analysis. To address this issue, we developed a novel Gaussian Process with Kernel Regression (GKR) method that allows study the simultaneously recorded neural population representation from an information geometry framework. We applied GKR to grid cell population activity, and found that running speed increases both grid cell activity toroidal-like manifold size and noise strength. Importantly, the effect of manifold dilation outpaces the effect of noise increasement, as indicated by the overall higher Fisher information at increasing speeds. This result is further supported by improved spatial information decoding accuracy at high speeds. Finally, we showed that the existence of noise covariance is information detrimental because it causes more noise projected onto the manifold surface. In total, our results indicate that grid cell spatial coding improves with increasing running speed. GKR provides a useful tool to understand neural population coding from an intuitive information geometric perspective.
A coral peptide has bactericidal activity against a global marine pathogen,Vibrio coralliilyticus
Authors: Aoyama Kako , Okai Masahiko , Ogawa Nobuhiro , Fukumaru Riko , Ishida Masami , Inoue Koji , Takagi Toshiyuki
Published: September 2024

Scleractinian corals and their associated microorganisms, including endosymbiotic dinoflagellates and bacteria, constitute coral holobionts. Rising seawater temperatures weaken coral symbiotic relationships, thereby increasing susceptibility to infectious diseases and leading to disease outbreaks and subsequent population declines. The temperature-dependent coral pathogen,Vibrio coralliilyticus, poses one of the greatest threats to coral reefs because of global warming. However, coral immune defenses against this pathogen are poorly understood. We previously identified coral genes responding toV. coralliilyticusexposure by transcriptomic analysis of the reef-building coral,Acropora digitifera. Here, bioinformatic analysis identified digitiferin, a coral antimicrobial peptide (AMP), in the genome ofA. digitifera. Recombinant digitiferin showed antibacterial activity against both Gram-positive and -negative bacteria. A 3D structural model and direct microscopic observations revealed that digitiferin damaged bacterial cell membranes by forming pores; however, initial growth inhibition tests in 1.5 % (w/v) NaCl showed no effect on pathogens. Because we found that digitiferin is secreted from epithelial cells into mucus and shows salt sensitivity, we hypothesized that it is active against pathogens in low-salt environments. We then investigated whether it showed antibacterial activity against pathogens in low-salt, moderate-osmolality conditions, using mannitol for osmoregulation instead of NaCl. The results showed that digitiferin exhibits bactericidal activity againstV. coralliilyticusunder salt-free conditions. To the best of our knowledge, this is the first study to characterize a scleractinian AMP that killsV. coralliilyticus. These findings contribute to a better understanding of coral immunity and may facilitate development of techniques to overcome coralVibriodisease.

Significance

Healthy coral reefs are one of the most important marine ecosystems, supporting ∼25 % of all marine organisms. Coral reef ecosystems are threatened by coral infectious diseases, as rising seawater temperatures exacerbate pathogen infectivity. Thus, understanding coral immune systems is becoming increasingly important to protect coral reefs. This study reports the discovery of an antimicrobial peptide, digitiferin, from the reef-building coral,Acropora digitifera. Digitiferin is effective against Gram-positive and -negative bacteria, including the major coral pathogen,Vibrio coralliilyticus, and is secreted into mucus from ectodermal granular epithelial cells. As the first antimicrobial peptide known to have bactericidal activity againstV. coralliilyticus, this discovery enhances our understanding of coral immunology and coral-pathogen interactions.
TYPE THREE SECRETION SYSTEM-INDUCED MECHANOPORATION OF VACUOLAR MEMBRANES
Authors: Swistak Léa , Albert Marvin , Valenzuela Camila , Gokerkucuk Elif Begum , Bontems François , Gazi Anastasia D. , Tachon Stéphane , Sartori-Rupp Anna , Lesser Cammie F. , Paul-Gilloteaux Perrine , Tinevez Jean-Yves , Vos Matthijn , Enninga Jost
Published: September 2024

Summary

Endomembrane breaching is a crucial strategy employed by intracellular pathogens enclosed within vacuoles to access the nutrient-rich cytosol for intracellular replication. While bacteria use various mechanisms to compromise host membranes, the specific processes and factors involved are often unknown.Shigella flexneri,a major human pathogen, accesses the cytosol relying on the Type Three Secretion System (T3SS) and secreted effectors. Using in-cell correlative light and electron microscopy, we tracked the sequential steps ofShigellahost cell entry. Moreover, we captured the T3SS, which projects a needle from the bacterial surface, in the process of puncturing holes in the vacuolar membrane. This initial puncture ensures disruption of the vacuolar membrane. Together this introduces the concept of mechanoporation via a bacterial secretion system as a crucial process for bacterial pathogen-induced membrane damage.

GraphicalAbstract
Neuronal synchronization inDrosophila
Authors: Fernandez-Chiappe Florencia , Wappner Marcos , Morelli Luis G. , Muraro Nara I.
Published: September 2024

Rhythms are intrinsic to biological processes across temporal and spatial scales. In the brain, the synchronized oscillatory activity of neurons creates collective rhythms that are essential for complex functions. While this is a recognized phenomenon in the mammalian brain, information about insect neuronal synchrony and its underlying mechanisms is scarce. In the fly brain, neuronal oscillations were reported in individual lateral ventral neurons (LNvs), which play a key role in circadian and sleep behaviors. However, it is still unclear whether and how these participate in a collective rhythm. In this work, we perform thorough whole-cell patch clamp recordings of LNvs, and demonstrate consistent membrane potential oscillations. We show that oscillations degrade over time, and disappear upon exposure to an acetylcholine receptor blocker. Together with a flat phase response curve, these results suggest that oscillations are exogenously produced. Prompted by these results, we propose a generic forced oscillator theory that can account for the experimental phase response. The theory further predicts that neurons with similar properties should oscillate in synchrony with zero lags, while neurons with different properties may show coherent oscillations with non-zero lags. We confirm this prediction through simultaneous patch clamp recordings of neuronal pairs, revealing that large LNvs are consistently advanced relative to small LNvs. Additionally, we find that other neurons in the accessory medulla also exhibit coherent membrane potential oscillations, with diverse lags. Our findings suggest the intriguing possibility that brain waves may arise from collective neuronal activity within this region of the fly brain.
The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma
Authors: Chen Ying-Jiun C. , Bhaskara Govinal Badiger , Lu Yue , Lin Kevin , Dent Sharon Y.R.
Published: September 2024

Despite recent advances in therapeutic treatments, multiple myeloma (MM) remains an incurable malignancy. Epigenetic factors contribute to the initiation, progression, relapse, and clonal heterogeneity in MM, but our knowledge on epigenetic mechanisms underlying MM development is far from complete. The SAGA complex serves as a coactivator in transcription and catalyzes acetylation and deubiquitylation. Analyses of data sets in the Cancer Dependency Map Project revealed that many SAGA components are selective dependencies in MM. To define SAGA-specific functions, we focused on ADA2B, the only subunit in the lysine acetyltransferase (KAT) module that specifically functions in SAGA. Integration of RNA sequencing (RNA-seq), assay for transposase-accessible chromatin with sequencing (ATAC-seq), and cleavage under targets and release using nuclease assay (CUT&RUN) results identified pathways directly regulated by ADA2B including MTORC1 signaling and oncogenic programs driven by MYC, E2F, and MM-specific MAF. We discovered that ADA2B is recruited to MAF and MYC gene targets, and that MAF shares a majority of its targets with MYC in MM cells. Furthermore, we found that the SANT domain of ADA2B is required for interaction with both GCN5 and PCAF acetyltransferases, incorporation into SAGA, and ADA2B protein stability. Our findings uncover previously unknown SAGA KAT module-dependent mechanisms controlling MM cell growth, revealing a vulnerability that might be exploited for future development of MM therapy.
Nonreciprocity of surface acoustic waves coupled to spin waves in a ferromagnetic bilayer with noncollinear layer magnetizations
Authors: Ushii Lidiia , Slavin Andrei , Verba Roman
Published: September 2024

Nonreciprocity of propagation of surface acoustic waves (SAWs) in the microwave frequency band can be achieved using the magnetoelastic interaction of SAWs with spin waves (SWs) propagating in magnetic heterostructures. Recent works have shown that the ultimate isolation of a counterpropagating hybridized SAW/SW is achieved in heterostructures consisting of a synthetic antiferromagnet—a ferromagnetic (FM) bilayer with antiferromagnetic Ruderman-Kittel-Kasuya-Yosida interlayer coupling—placed on top of a piezoelectric acoustic waveguide. In this work, we study in detail a more practical and technologically simpler system based on an FM bilayer, where layers are coupled by only dipole-dipole interaction, and having noncollinear magnetizations of the FM layers. A weak in-plane anisotropy with noncollinear easy axes in the layers is shown to be the only essential factor for the realization of strongly nonreciprocal propagation of a hybridized SAW/SW. We formulate requirements for the relative orientation of the layer’s magnetizations and wave propagation direction necessary to realize an efficient SAW isolator, and demonstrate examples of SAW transmission characteristics which prove the possibility of achieving an isolation exceeding 50 dB for a submillimeter-long FM bilayer with insertion losses of just a few decibels more than those of a pure SAW device. In addition to relative fabrication simplicity, the proposed magnetoelastic heterostructure exhibits a reasonable robustness in respect to deviations in the anisotropy axes and/or bias field directions—an important benefit for device mass production.

Published by the American Physical Society
2024
Structure of MltG from Mycobacterium abscessus reveals structural plasticity between composed domains
Authors: Lee Gwan Hee , Kim Subin , Kim Do Yeon , Han Ju Hee , Lee So Yeon , Lee Jun Hyuck , Lee Chang Sup , Park Hyun Ho
Published: September 2024

MltG, a membrane-bound lytic transglycosylase, has roles in terminating glycan polymerization in peptidoglycan and incorporating glycan chains into the cell wall, making it significant in bacterial cell-wall biosynthesis and remodeling. This study provides the first reported MltG structure from Mycobacterium abscessus (maMltG), a superbug that has high antibiotic resistance. Our structural and biochemical analyses revealed that MltG has a flexible peptidoglycan-binding domain and exists as a monomer in solution. Further, the putative active site of maMltG was disclosed using structural analysis and sequence comparison. Overall, this study contributes to our understanding of the transglycosylation reaction of the MltG family, aiding the design of next-generation antibiotics targeting M. abscessus.
Age-friendly human resource practices: a comparison of Baltic and Nordic countries
Authors: Dorokhov Oleksandr , Jaakson Krista , Dorokhova Liudmyla
Published: September 2024

Purpose

Due to population ageing, the European Union (EU) has adopted active ageing as a guiding principle in labour and retirement policies. Among the strategies for active ageing, age-friendly workplaces play a crucial role. This study compares age-friendly human resource (HR) practices in the Baltic and Nordic countries. The latter are pioneers in active ageing, and as the employment rate of older employees in the Baltics is like that in the Nordic countries, we may assume equally age-friendly workplaces in both regions.

Design/methodology/approach

We used the latest CRANET survey data (2021–2022) from 1,452 large firms in seven countries and constructed the fuzzy logic model on age-friendliness at the workplace.

Findings

Despite a high employment rate of older individuals in the Baltics, HR practices in these countries fall short of being age-friendly compared to their Nordic counterparts. Larger firms in the Nordic countries excel in every studied aspect, but deficiencies in the Baltics are primarily attributed to the absence of employer-provided health and pension schemes. The usage of early retirement is more frequent in the Nordic countries; however, its conceptualisation as an age-friendly HR practice deserves closer examination. Our findings suggest that the success of active ageing in employment has translated into age-friendly HR practices in larger organisations in the Nordics, but not in the Baltics. It is likely that high employment of older individuals in Estonia, Latvia and Lithuania is a result of the relative income poverty rate.

Originality/value

Our model represents one of the few attempts to utilise fuzzy logic methodology for studying human resource practices and their quantitative evaluation, especially concerning age-friendly workplaces.
Impact of agricultural land ownership on child nutrition in rural Sudan: an investigation across gender
Authors: Mohamed Nour Samia , Ebaidalla Ebaidalla M.
Published: September 2024

Purpose

In light of the inequality in access to farming land and the high prevalence of child malnutrition in Sudan, there is a lack of empirical research on the relationship between land ownership and child nutritional status. This study aims to examine the influence of agricultural landholding on the nutritional status of children under the age of five in rural Sudan.

Design/methodology/approach

The study utilizes data from Sudan’s 2014 Multiple Indicator Cluster Survey (MICS), covering a sample of 10,753 rural children. The empirical analysis uses the two-stage least squares (2SLS) approach, adopting various estimation methods and model specifications for robustness check and comparison.

Findings

The results demonstrate that agricultural land has a positive and significant effect on reducing child malnutrition, signifying that children from families with agricultural land are less susceptible to malnutrition in Sudan. When examining the male and female sub-samples separately, the findings indicate a positive influence of land ownership on child malnutrition in the female sub-sample, while no significant impact is observed in the male sub-sample. This indicates a gender disparity in the effects of land ownership on child nutrition, with girls benefiting more from access to agricultural land compared to boys.

Originality/value

The study has several significant contributions. First, this is the sole study that examines the impact of agricultural land ownership on child malnutrition in Sudan. Second, considering the gender variations in nutritional status, investigating the influence of land ownership on child nutrition across genders addresses a significant gap in the current literature. Finally, the findings resulting from this study can contribute to achieving the United Nations Sustainable Development Goals (SDGs) to be achieved by 2030, precisely focusing on SDG2 Goal 2: Zero hunger and SDG 10: Goal 10: Reduced inequalities.
The effect of involvement and perceived usefulness on attitudinal and behavioral loyalty in online bookings
Authors: Goyal Anita , Verma Pranay
Published: September 2024

Purpose

This study aims to examine involvement and perceived usefulness (PU) as the drivers of loyalty for online booking websites, revealing findings of theoretical and practical relevance. The proposed conceptual model is drawn on elaboration likelihood model (ELM) along with the consideration of expectation confirmation model for loyalty development.

Design/methodology/approach

A two-stage structural equation modeling was applied to test the reliability and validity of the constructs and the strength of the hypothesized relationships.

Findings

Attitudinal loyalty (AL) is found to have a significant role, resulting in behavioral loyalty (BL) toward online booking. The results of this study supported the ELM framework, where involvement and PU resulted in BL through AL. PU and AL also served as mediating mechanisms underlying the impact of consumer involvement on BL.

Originality/value

This study applies the ELM to online ticketing mechanisms and nuances amid AL and BL with their drivers.
Augmented reality marketing in hospitality and tourism: a guide for researchers and managers
Authors: tom Dieck M. Claudia , Han Dai-In Danny , Rauschnabel Philipp A.
Published: September 2024

Purpose

The hospitality and tourism industry is strongly influenced by new and immersive technologies, such as augmented reality (AR), to enhance customer experiences across a diverse set of touchpoints throughout the visitor journey. This paper aims to provide a holistic understanding of AR marketing for this industry context, present a number of fundamental premises of AR marketing within it and establish an agenda for future AR research.

Design/methodology/approach

This study reviews current literature on AR marketing, hospitality and tourism and industry use cases for the creation of a proposed conceptual framework to guide scholars and managers. Based on that, the authors propose fundamental premises.

Findings

The three fundamental premises of AR marketing presented are the need to clearly differentiate between AR and virtual reality within hospitality and tourism; the use of AR for the on-trip experience; and the combined focus on content, context, customer and computing devices for a successful strategic implementation of AR.

Research limitations/implications

This study serves as a first point of reference for the strategic integration of AR into hospitality and tourism marketing, both from an industry and academic point of view.

Practical implications

The authors provide a number of managerial recommendations based on our three fundamental premises.

Originality/value

To the best of the authors’ knowledge, this study is one of the first to holistically characterize AR marketing in the hospitality and tourism context. It also highlights the fundamental premises of successful AR marketing and future directions of AR research today and in a spatial computing future.
Channel-switching behaviour and customer deviance
Authors: Mayr Kathrin , Teller Christoph
Published: September 2024

Purpose

Unacceptable behaviour in retailing – negative customer deviance (NCD) is rising, damaging retailers financially. Current research investigates forms of NCD by addressing its impact on employees but overlooks its effects on bystander-customers and their retail channel preferences. As channel switching within retailing is increasing unprecedentedly, this research investigates its correspondence with NCD encounters.

Design/methodology/approach

This research uses structural equation modelling, based on data collection administered through a web-based survey of 1,008 customers of at least 16 years of age, to analyse the research model.

Findings

The findings reveal unexplored forms of NCD perceived by bystander-customers in retailing and their consequences, linking it to bystander-customers' ill-being, dissatisfaction with the shopping experience, a decrease in store commitment and an increase in their retail channel-switching intentions. Additionally, the research uncovers moderating variables.

Practical implications

This research tests NCD dimensions and effects on bystander-customers, which indicate the need for retailers to address shopping values, attitudes and commitment through corrective, proactive and long-term strategic actions.

Originality/value

As one of the first studies to investigate the impact of NCD on bystander-customers' intentions to switch from store-based to online shopping, strategies for retailers are developed to help diminish and control NCD-induced threats to bystander-customers.
Empowering the elderly: a cash waqf Takaful model for enhanced provision and protection in Malaysia
Authors: Muneeza Aishath , Kunhibava Sherin , Mohamed Ismail , Mustapha Zakariya
Published: September 2024

Purpose

The primary objective of this research is to introduce a pioneering takaful model that provides both provision and protection to the aging population by combining the concept of cash waqf with takaful. This model is designed to align with Shariah principles, ensuring sustainability and enduring impact.

Design/methodology/approach

This research adopts a qualitative methodology, where a focus group discussion was conducted with six stakeholders. The participants consisted of takaful operators, legal experts and other industry players. The participants were presented with the proposed cash waqf takaful model and their feedback was recorded. Legal issues related to linking waqf with takaful were also identified and discussed.

Findings

The study highlights the need for innovative financial solutions to support Malaysia's aging population. It proposes a cash waqf takaful model, leveraging crowd funding for sustainability. Legal hurdles and recommendations for overcoming them are discussed, along with suggestions for future research on quantitative validation and regulatory frameworks. Ultimately, the study emphasizes the holistic approach of the proposed model in addressing the well-being of Malaysia's senior citizens.

Practical implications

The proposed takaful model presents opportunities for takaful operators to integrate Islamic social finance into their operations, enabling easier access to takaful for the elderly community. By eliminating financial barriers, it can transform the takaful landscape, ensuring inclusivity and financial security for aging populations. Moreover, policymakers see it as a blueprint for sustainable financial solutions and social welfare enhancement globally.

Originality/value

The study introduces a novel cash waqf takaful model to support Malaysia's aging population, leveraging crowdfunding for sustainability. It addresses legal challenges unique to Malaysia and proposes collaboration with State Islamic Religious Authorities. Furthermore, it emphasizes the need for further research to validate the model's effectiveness and explores its potential global policy implications.
The digital library of Arab children's culture for sustainable development (DLACSD)
Authors: Youssef Loubna A. , Elsayed Usama , Shaheen Sherif , Khalifa Nour Mahmoud
Published: September 2024

Purpose

This paper focuses on a project to work on the digital library of Arab children's culture for sustainable development (DLACSD).

Design/methodology/approach

This project claims to link the past, present, and future by creating a platform that can grow to include not only works by adults but by children who inspire adults with their imagination and the joys they bring to the world.

Findings

This project addresses in phases the different aspects of the problem of the lack of material for Egyptian/Arab children at different stages in Arabic on the internet (with copyright law in mind). It is time to fill this gap by having a rich repository of stories, plays, games and songs for children in Arabic in a digital library to enrich the life of the child and to inform the world that much that is worthwhile is available in Arabic for parents, teachers, and children to enjoy.

Research limitations/implications

Through reading samples of the works by Abdel-Tawab Youssef (1928–2015) by using the Dublin Core Elements, it will be informative to see how his writings address the United Nations Goals of Sustainable Development way before these Goals were discussed.

Practical implications

Writers for children, librarians, teachers, psychologists, literary critics, illustrators, and parents need a platform that makes material available to promote children’s culture in the Arab world and to introduce the world to what is of value for children in Arabic.

Social implications

Currently, communication brings the world together and although the social media and the new technology have introduced problems that are serious, to say the least, collaborators on all levels must play an active role in redressing the social wrongs, especially those affecting children.

Originality/value

This ongoing project by members of a team who believe in interdisciplinarity and multidisciplinarity has taken the first step to create and develop (DLACSD).
Nexus between customer relationship management dimensions, customer involvement and customer retention: a mediation analysis in Ghana’s hotel industry
Authors: Opoku Richard Kofi , Agyapong Gloria Kakrabah-Quarshie , Alhassan Abdulai
Published: September 2024

Purpose

This research investigates the role of customer involvement (CINV) in customer relationship management (CRM) dimensions and customer retention (CR) in Ghana’s hotel industry.

Design/methodology/approach

This quantitative-based explanatory research obtained primary data via structured questionnaires from 277 hotel customers in Ghana, processed it with SmartPLS4.0 software, and analysed it with structural equation modelling.

Findings

CRM dimensions (CRM-based technology, managing knowledge and personalisation of services) and CINV positively affect CR. Also, CINV partially mediates the interactions between the CRM dimensions and CR in Ghana’s hotel industry.

Research limitations/implications

The study is geographically limited to hotels in Ghana and conceptually limited to three CRM dimensions, CINV and CR. Methodologically, the study was limited to the quantitative approach. However, our outcomes imply that hotels in Ghana that invest in relevant CRM dimensions would improve CR. CRM-CR association can also be improved through CINV.

Practical implications

The study outcomes imply that when Ghanaian hotels implement the CRM dimensions and foster active CINV, their customers’ retention will improve significantly. Hence, CRM and CINV are prerequisites for enhancing CR in Ghana’s hotel industry.

Originality/value

The study offers valuable contributions to the current literature on CRM, consumer behaviour and hospitality management, especially in a developing economy context. Its novel contribution, the mediating role of CINV, would advance CRM studies in the hospitality sector.
The mediating role of halal supply chain integrity in enhancing performance for halal herbal-based food SMEs
Authors: Rajendran Salini Devi , Kamarulzaman Nitty Hirawaty , Abd Rahman Azmawani
Published: September 2024

Purpose

This paper aims to examine the influence of supply chain management by assessing the relationship between internal and external integration and small and medium enterprises (SMEs) owners’ Islamic practices in enhancing halal supply chain integrity (HSCI) and SMEs’ performance.

Design/methodology/approach

A total of 176 SMEs were surveyed using a self-administered questionnaire. The sample was selected using convenience sampling from two major halal exhibition events in Malaysia. Structural equation modeling (SEM) was used to analyze the data and test the hypotheses.

Findings

The findings showed that supply chain integration (SCI), Islamic human capital and HSCI have a significant relationship with SMEs’ performance. It was also found that HSCI mediated the relationship between both SCI and Islamic human capital and SMEs’ performance.

Practical implications

SME owners or managers should be committed to developing the internal processes within the organization and strategizing to link these processes with the external processes to obtain the full benefits of integration. Furthermore, as the upper management, owners and managers must understand the supply chain challenges, priorities and practices thoroughly, as they are responsible for Islamic business ethics. They should work to provide support to increase religious orientation in the SMEs, as this would likely enhance all other factors.

Originality/value

This is one of the few types of research to use HSCI as a mediator in halal food studies in addition to improving SMEs’ performance.
Modelling for working capital efficiency: integrating SBM-DEA and artificial neural networks in Indian manufacturing
Authors: Seth Himanshu , Tripathi Deepak Kumar , Chadha Saurabh , Tripathi Ankita
Published: September 2024

Purpose

This study aims to present an innovative predictive methodology that transitions from traditional efficiency assessment techniques to a forward-looking strategy for evaluating working capital management(WCM) and its determinants by integrating data envelopment analysis (DEA) with artificial neural networks (ANN).

Design/methodology/approach

A slack-based measure (SBM) within DEA was used to evaluate the WCME of 1,388 firms in the Indian manufacturing sector across nine industries over the period from April 2009 to March 2024. Subsequently, a fixed-effects model was used to determine the relationships between selected determinants and WCME. Moreover, the multi-layer perceptron method was applied to calculate the artificial neural network (ANN). Finally, sensitivity analysis was conducted to determine the relative significance of key predictors on WCME.

Findings

Manufacturing firms consistently operate at around 50% WCME throughout the study period. Furthermore, among the selected variables, ability to create internal resources, leverage, growth, total fixed assets and productivity are relatively significant vital predictors influencing WCME.

Originality/value

The integration of SBM-DEA and ANN represents the primary contribution of this research, introducing a novel approach to efficiency assessment. Unlike traditional models, the SBM-DEA model offers unit invariance and monotonicity for slacks, allowing it to handle zero and negative data, which overcomes the limitations of previous DEA models. This innovation leads to more accurate efficiency scores, enabling robust analysis. Furthermore, applying neural networks provides predictive insights by identifying critical predictors for WCME, equipping firms to address WCM challenges proactively.
A call to integrate non‐visual functions of pigments and their interactions with visual functions to understand global change impacts on visual systems
Authors: Reinke Beth A. , Avery Julian D. , Hua Jessica
Published: September 2024

Animal coloration serves a variety of visually related functions in nature (e.g. mate choice, aposematism and camouflage) but the pigments in integumentary tissues such as skin, scales and feathers may also serve functions unrelated to the visual environment (e.g. temperature regulation, detoxification and pollutant protection). Our understanding of the significance of the non‐visual functions of animal integumentary pigments, as well as how they interact with the visually related functions to shape animal visual systems, remains limited.

Furthermore, due to their important roles in shaping species interactions and mediating interactions in the environment, animal colour traits are likely to be impacted by global change (e.g. increased temperatures, altered habitat quality and quantity, increased environmental stochasticity, pollutants and novel species assemblages).

Considering the effects of global change on both visual and non‐visual functions is important for understanding whether the selection is acting directly on the pigment or on coloration. Since changing the trait distributions can then lead to changes in visual systems, we advocate for studies to consider all potential functions of integumentary pigments, both visual and non‐visual functions and their interaction.

Towards this goal, we first highlight common functions of pigments with a focus on non‐visual functions across animal systems. Then we synthesize our current understanding of how global change can impact pigmentation and discuss factors that can modify the interactions between climate change and pigment function. Lastly, we discuss how changes in colour traits can impact visual systems and provide an example using amphibians and their responses to climate change as a model.

Read the free Plain Language Summary for this article on the Journal blog.
Morph‐linked variation in female pheromone signalling and male response in a polymorphic moth
Authors: De Pasqual Chiara , Selenius Eetu , Burdfield‐Steel Emily , Mappes Johanna
Published: September 2024

Understanding the maintenance of genetic variation in reproductive strategies and polymorphisms in the wild requires a comprehensive examination of the complex interactions between genetic basis, behaviour and environmental factors.

We tested the association between three colour genotypes and variation in female pheromone signalling and male antennal morphology in the wood tiger moth (Arctia plantaginis). These moths have genetically determined white (WW, Wy) and yellow (yy) hindwings that are linked to mating success and fitness, with heterozygotes (Wy) having an advantage. We hypothesized that attractiveness and reproductive success are correlated, with Wy females being more attractive than the other two genotypes which could contribute to maintaining the polymorphism.

Female attractiveness was tested by baiting traps with females of the three colour genotypes both in low‐ (i.e. field set‐up) and in high‐population density (i.e. large enclosure set‐up). Male's ability to reach females was correlated to their own colour genotype and antennal morphology (length, area and lamellae count).

Contrary to our prediction, morph‐related reproductive success and attractiveness were not correlated. Heavier Wy females attracted a lower proportion of males compared to WW and yy females. Specifically, an increase in weight corresponded to a decreased Wy but increased yy female attractiveness. yy females were generally more attractive than others likely due to earlier pheromone release. In males, lamellae count and genetic colour morph were linked to the male's ability to locate females. Furthermore, male traits affected their ability to reach females in a context‐specific way. Males with denser antennae (i.e. higher lamellae count) and white males reached the females faster than yellows in the enclosure, while yellow males located females faster than whites in the field.

Our results indicate that higher yy female attractiveness was likely affected by the combined effect of early pheromone release, female weight and higher population density. Males' searching success was affected by morph‐specific behavioural strategies and local population density. Ultimately, the combined effect of genotype‐related pheromone signalling strategies of females together with environment‐dependent male behaviour affect male response and potentially contribute to maintaining variation in fitness‐related traits.