bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Simultaneous Application of Glass Fiber Reinforcement and Polymer-Modified Asphalt Emulsion on DBST’s Resistance to Aggregate Loss Using Laboratory Investigation
Authors: Kim Je Won , Elipse Carlo
Published: June 2024

Double bituminous surface treatment (DBST) has been a widely utilized pavement maintenance material due to its capability to restore the surface roughness of existing pavement and provide a layer of protection against weathering, aging, and moisture. However, DBST is highly prone to aggregate loss at an early stage, which is a very common problem experienced by surface treatment. Therefore, to lessen the aggregate loss and prolong the service life of DBST, fiber additive can be incorporated to strengthen the adhesion between the asphalt emulsion and aggregates. This study investigated the performance of glass fiber-reinforced polymer-modified DBST against aggregate loss by conducting laboratory tests using typical DBST as the benchmark of the test results. Four laboratory tests were chosen to represent different loading applications on the surface of the pavement: the bitumen bond strength (BBS) test, the sweep test, the Hamburg wheel-track test (HWT test), and a one-third-scale model mobile load simulator (MMLS3) model. Furthermore, the curing time of the asphalt emulsion was considered in the BBS test and sweep test. Based on all results from the conducted laboratory tests, polymer-modified DBST with glass fiber reinforcement presented an increased resistance to aggregate loss compared with typical DBST. Moreover, it was found that a longer curing time of the asphalt emulsion, whether it was typical or modified, strengthened the surface treatment’s resistance to aggregate loss.
A Survey Detailing Early Onset Colorectal Cancer Patient and Caregiver Experiences in Canada
Authors: Auer Rebecca , Meszaros Claudia , Fossouo Lucresse , Vandermeer Lisa , Stein Barry D.
Published: June 2024

The incidence of early onset colorectal cancer (EOCRC) in Canada has increased. To address the growing incidence of EOCRC, Colorectal Cancer Canada (CCC) developed the Never Too Young (N2Y) program to identify gaps in care and evaluate patient and caregiver experiences with CRC. The survey was available online using SurveyMonkey across Canada between 12 December 2022 and 1 May 2023. The patient and caregiver survey consisted of 113 and 94 questions, respectively. A total of 108 EOCRC patients and 20 caregivers completed the survey. Many respondents were unaware of EOCRC (41.6%) and the disease symptoms (45.2%) before diagnosis. Patient age at diagnosis was between 45 and 50 years in 31.7%, and 72.8% of them were diagnosed at stage III or IV. A perception of an initial misdiagnosis was common (67.4%) for EOCRC patients, and 51.2% felt dismissed due to their age. Patients and caregivers reported impacts of EOCRC on their mental health, with 70.9% of patients expressing a need for support with depression and 93.3% of caregivers experiencing a constant fear of recurrence of their loved one’s cancer. Improving the Canadian population’s awareness of EOCRC (e.g., CRC symptoms) is important for ensuring timely diagnoses. Similarly, it is critical to ensure that healthcare providers are aware of the increase in EOCRC cases and the unique needs of these patients. Re-evaluation of the CRC screening age should be undertaken in Canada to determine whether lowering the start age to 45 years will improve outcomes in this demographic.
Clinical Applications of Comprehensive Genomic Profiling in Advanced Non-Small-Cell Lung Cancer—A Case Series
Authors: Tsai Chun-Ming , Lin Chih-Hung , Chou Yu-Yen , Jen Hsiao-Yu , Jain Suyog
Published: June 2024

Background: Advanced non-small-cell lung cancer (NSCLC) can be treated with novel targeted therapies that are tailored to the genetic characteristics of malignancy. While tissue-based genomic testing is considered the gold standard for the detection of oncogenic driver mutations, several challenges like inadequate tissue availability, the invasiveness of procuring tumors, and prolonged turnaround time of analysis are encountered. Considering these limitations, guidelines have recognized liquid biopsies using circulating cell-free DNA (cfDNA) as a useful tool to complement conventional tissue testing. Even though cfDNA next-generation sequencing (NGS) can have high sensitivity and specificity, optimal patient benefit requires the interpretation of the molecular profiling results in the context of clinical and diagnostic features to achieve the best outcomes. Case Descriptions: In this case series, we present six patients with advanced NSCLC whose plasma or tissue biopsy samples were analyzed with commercially available comprehensive NGS assays that elucidate the role of testing at various time points in the treatment journey. In all six cases, comprehensive genomic profiling (CGP) provided clinically useful information to guide treatment decisions. Conclusion: Adding to the existing real-world evidence, this case series reinforces that CGP-driven treatment strategies in advanced NSCLC, coupled with other available clinical information, can optimize treatment decisions.
Determination of Permanent Deformations of Non-Cohesive Soils in Pavement Structures under Repeated Traffic Load
Authors: Vamos Mate Janos , Szendefy Janos
Published: June 2024

One of the main types of distress in pavement structures is rutting, which may also reduce serviceability significantly. Most design methods typically attribute rutting to the asphalt layer alone, proposing that it can be managed by controlling vertical deformation or stress at the subgrade’s top. Furthermore, these methods frequently lack precise measurements for rut depth. On-site measurements show that the majority of permanent deformation occurs in the unbound layers beneath the asphalt; attention should be directed towards these layers. In recent literature, there are calculation methods that account for accumulating strains in soils. However, further investigation is needed regarding the effect of soil properties and the significance of the pavement cross-section. The literature is also somewhat contractionary regarding the origin of permanent deformations. In this research, the residual settlement of soils (base, subbase, and subgrade) under flexible pavement systems was analyzed due to the repeated traffic loads. Rut depths were calculated and analyzed using the High-Cycle Accumulation (HCA) model. The different behaviour in each course of the pavement system is discussed to reveal their contribution to total ruts. The effect of the grain size distribution of the subgrade was analyzed, and its significance on the rutting depths was demonstrated. Standardized pavement cross-sections with different asphalt thicknesses were evaluated, and the calculated settlements of the pavement originating from the ground during the design lifetime are also presented. It is shown that, with the same number of repetitions, the settlements calculated in each traffic load class are proportional to the thickness of the asphalt course. The contribution of the base, subbase, and subgrade courses to the total settlement is also presented for different subgrade types and traffic load classes.
Functional Segregation of Resource Utilization Strategies between Invasive and Native Plants and Invasion Mechanisms in the Water Level Fluctuation Zone: A Case Study of Pengxi River in Three Gorges Reservoir, China
Authors: Cheng Lideng , Yuan Xingzhong , Sun Kuo , Li Peiwu
Published: June 2024

The ecosystem of the water level fluctuation (WLF) zone of the Three Gorges Reservoir (TGR) is highly vulnerable and sensitive due to its unique cyclical flooding and drought conditions. The ecological impact of biological invasion in this area is particularly severe, making it crucial to study the differences in resource utilization strategies between invasive plants (IPs) and native plants (NPs) using functional traits to explore the mechanisms of invasion. We selected the WLF zone of Pengxi River in the TGR area and conducted a multi-scale study along the elevation gradient. The results reveal that at the regional scale, IPs have a larger height and specific leaf area, smaller leaf tissue density, and specific root length compared to NPs, showing a preference for enhancing aboveground resource acquisition over leaf defense capabilities. They allocate more tissue construction resources to their roots to withstand environmental pressures, which may be the key to their successful intrusion, highlighting the role of niche differentiation. On the community scale, the H and SLA of IPs and NPs are positively correlated with elevation, while the LTD of IPs shows a negative correlation. At elevations of 175 m and below, IP and NP exhibit functional convergence, while above 175 m, functional divergence was observed. This indicates that although the different resource utilization strategies are crucial for successful IP invasion, the environmental filtering from periodic floods and drought pressures play a significant role in community assembly in the WLF zone, allowing IP to integrate into habitats with similar functional characteristics already inhabited by NP and establish their own communities.
Evaluation of Deterioration Degree of Archaeological Wood from Luoyang Canal No. 1 Ancient Ship
Authors: Yang Weiwei , Ma Wanrong , Liu Xinyou
Published: June 2024

This study provides a detailed investigation of archaeological wood samples from the Luoyang Canal No. 1 site, focusing on wood species identification, physical properties, mechanical property analyses, and morphological examination. The identified wood species, belonging to the Ulmus genus, exhibited a 43% decline in compressive strength in waterlogged environments. Further, the wood exhibited increased moisture content, higher porosity, reduced basic density, and elevated shrinkage rates, indicating a mild level of degradation. X-ray diffraction was employed for the observation of cellulose structure, and Fourier transform infrared spectroscopy (FT-IR) demonstrated significant removal of cellulose and hemicellulose components. These findings emphasize the importance of understanding wood degradation mechanisms to evaluate structural integrity and durability in guiding the development of effective preservation strategies for archaeological wood artifacts. Continued research and conservation are crucial to deepen our knowledge of wood deterioration processes and enhance the implementation of preservation techniques.
Anomalous Warm Temperatures Recorded Using Tree Rings in the Headwater of the Jinsha River during the Little Ice Age
Authors: Jiang Chaoling , Xu Haoyuan , Tong Yuanhe , Li Jinjian
Published: June 2024

As a feature of global warming, climate change has been a severe issue in the 21st century. A more comprehensive reconstruction is necessary in the climate assessment process, considering the heterogeneity of climate change scenarios across various meteorological elements and seasons. To better comprehend the change in minimum temperature in winter in the Jinsha River Basin (China), we built a standard tree-ring chronology from Picea likiangensis var. balfouri and reconstructed the regional mean minimum temperature of the winter half-years from 1606 to 2016. This reconstruction provides a comprehensive overview of the changes in winter temperature over multiple centuries. During the last 411 years, the regional climate has undergone seven warm periods and six cold periods. The reconstructed temperature sensitively captures the climate warming that emerged at the end of the 20th century. Surprisingly, during 1650–1750, the lowest winter temperature within the research area was about 0.44 °C higher than that in the 20th century, which differs significantly from the concept of the “cooler” Little Ice Age during this period. This result is validated by the temperature results reconstructed from other tree-ring data from nearby areas, confirming the credibility of the reconstruction. The Ensemble Empirical Mode Decomposition method (EEMD) was adopted to decompose the reconstructed sequence into oscillations of different frequency domains. The decomposition results indicate that the temperature variations in this region exhibit significant periodic changes with quasi-3a, quasi-7a, 15.5-16.8a, 29.4-32.9a, and quasi-82a cycles. Factors like El Niño–Southern Oscillation (ENSO), Pacific Decadal Oscillation (PDO), and solar activity, along with Atlantic Multidecadal Oscillation (AMO), may be important driving forces. To reconstruct this climate, this study integrates the results of three machine learning algorithms and traditional linear regression methods. This novel reconstruction method can provide valuable insights for related research endeavors. Furthermore, other global climate change scenarios can be explored through additional proxy reconstructions.
Improving Forest Above-Ground Biomass Estimation by Integrating Individual Machine Learning Models
Authors: Luo Mi , Anees Shoaib Ahmad , Huang Qiuyan , Qin Xin , Qin Zhihao , Fan Jianlong , Han Guangping , Zhang Liguo , Shafri Helmi Zulhaidi Mohd
Published: June 2024

The accurate estimation of forest above-ground biomass (AGB) is crucial for sustainable forest management and tracking the carbon cycle of forest ecosystem. Machine learning algorithms have been proven to have great potential in forest AGB estimation with remote sensing data. Though many studies have demonstrated that a single machine learning model can produce highly accurate estimations of forest AGB in many situations, efforts are still required to explore the possible improvement in forest AGB estimation for a specific scenario under study. This study aims to investigate the performance of novel ensemble machine learning methods for forest AGB estimation and analyzes whether these methods are affected by forest types, independent variables, and spatial autocorrelation. Four well-known machine learning models (CatBoost, LightGBM, random forest (RF), and XGBoost) were compared for forest AGB estimation in the study using eight scenarios devised on the basis of two study regions, two variable types, and two validation strategies. Subsequently, a hybrid model combining the strengths of these individual models was proposed for forest AGB estimation. The findings indicated that no individual model outperforms the others in all scenarios. The RF model demonstrates superior performance in scenarios 5, 6, and 7, while the CatBoost model shows the best performance in the remaining scenarios. Moreover, the proposed hybrid model consistently has the best performance in all scenarios in spite of some uncertainties. The ensemble strategy developed in this study for the hybrid model substantially improves estimation accuracy and exhibits greater stability, effectively addressing the challenge of model selection encountered in the forest AGB forecasting process.
Elevation Shapes Soil Microbial Diversity and Carbon Cycling in Platycladus orientalis Plantations
Authors: Zhang Yushu , Yuan Chao , Hu Dongyang , Zhang Yong , Hou Lina , Li Jinyu , Han Siyu , Dou Yuanyang , Cao Jixin
Published: June 2024

Diversified soil microbiomes are the key drivers of carbon fixation and plant residue decomposition in forest ecosystems. Revealing the elevation patterns of soil microbial carbon cycling in forests is essential for utilization of forest ecological resources. However, the soil microbial diversity and carbon cycle processes in Platycladus orientalis plantations across different elevations are still unclear. Here, we established a gradient with three elevations (118 m, 300 m, and 505 m) on the Beijing Ming Dynasty Tombs Forest Farm, which is located in Changping District, Beijing. The metagenomics method was applied to study the soil microbiome, with a special focus on the carbon cycle process at each elevation. We found the diversity and composition of the soil microbiomes significantly varied across the elevation gradients. The structure of bacteria and archaea was mainly driven by soil total potassium, pH and NH4+, but the eukaryota had no significant relationship with the environmental factors. The relative abundance of genes involved in microbial carbon fixation and decomposition of organic carbon were also significantly impacted by elevation, with the former showing increasing, u-shaped, or hump trends with increasing elevation, but the latter only showing hump trends. The rTCA cycle and 3-hydroxypropionate pathway were the dominant carbon fixation pathways in the Platycladus orientalis plantations. The elevation gradient shaped the microbial decomposition of plant-derived organic carbon by changing soil properties and, furthermore, led to soil organic carbon stock losses. These findings increase our understanding of soil microbial diversity and the carbon cycle across different elevations and provide a theoretical basis for the utilization of forest ecological resources to promote carbon sequestration.
In-Home Evaluation of the Neo Care Artificial Intelligence Sound-Based Fall Detection System
Authors: Maher Carol , Dankiw Kylie A. , Singh Ben , Bogomolova Svetlana , Curtis Rachel G.
Published: June 2024

The Neo Care home monitoring system aims to detect falls and other events using artificial intelligence. This study evaluated Neo Care’s accuracy and explored user perceptions through a 12-week in-home trial with 18 households of adults aged 65+ years old at risk of falls (mean age: 75.3 years old; 67% female). Participants logged events that were cross-referenced with Neo Care logs to calculate sensitivity and specificity for fall detection and response. Qualitative interviews gathered in-depth user feedback. During the trial, 28 falls/events were documented, with 12 eligible for analysis as others occurred outside the home or when devices were offline. Neo Care was activated 4939 times—4930 by everyday household sounds and 9 by actual falls. Fall detection sensitivity was 75.00% and specificity 6.80%. For responding to falls, sensitivity was 62.50% and specificity 17.28%. Users felt more secure with Neo Care but identified needs for further calibration to improve accuracy. Advantages included avoiding wearables, while key challenges were misinterpreting noises and occasional technical issues like going offline. Suggested improvements were visual indicators, trigger words, and outdoor capability. The study demonstrated Neo Care’s potential with modifications. Users found it beneficial, but highlighted areas for improvement. Real-world evaluations and user-centered design are crucial for healthcare technology development.
Nutritional Barriers to the Adherence to the Mediterranean Diet in Non-Mediterranean Populations
Authors: Sam-Yellowe Tobili Y.
Published: June 2024

Adherence to the Mediterranean diet has been shown to lower the risk of developing chronic non-communicable diseases like cardiovascular and neurodegenerative diseases and cancer. Improvements in depression, participation in daily activities in older individuals, weight loss and a reduction in adverse pregnancy outcomes are associated with adherence to the Mediterranean diet. The number of studies that have evaluated barriers to adherence to the Mediterranean diet in the US and, in particular, in racial and ethnic minority populations within the US are few. Among Native American and Alaskan Native populations, studies evaluating traditional or alternative Mediterranean diet adherence for chronic non-infectious diseases is unavailable. Mediterranean diet scoring instruments used in studies in European and Mediterranean countries and among white participants in the US fail to capture the dietary patterns of racial and ethnic minority populations. In this narrative review, the food components of the traditional Mediterranean diet are discussed, adherence to the Mediterranean diet is examined in Mediterranean and non-Mediterranean countries and barriers preventing adherence to the Mediterranean diet in the US and among racial and ethnic minority populations is reviewed. Recommendations for improving nutrition education and intervention and for increasing adherence and cultural adaptions to the Mediterranean diet are provided.
Prediction of Potential Distribution of Carposina coreana in China under the Current and Future Climate Change
Authors: Zhang Guolei , Liu Sai , Xu Changqing , Wei Hongshuang , Guo Kun , Xu Rong , Qiao Haili , Lu Pengfei
Published: June 2024

Carposina coreana is an important pest of Cornus officinalis, distributed in China, Korea, and Japan. In recent years, its damage to C. officinalis has become increasingly serious, causing enormous economic losses in China. This study and prediction of current and future suitable habitats for C. coreana in China can provide an important reference for the monitoring, early warning, prevention, and control of the pest. In this study, the potential distributions of C. coreana in China under current climate and future climate models were predicted using the maximum entropy (MaxEnt) model with ArcGIS software. The distribution point data of C. coreana were screened using the buffer screening method. Nineteen environmental variables were screened using the knife-cut method and variable correlation analysis. The parameters of the MaxEnt model were optimized using the kuenm package in R software. The MaxEnt model, combined with key environmental variables, was used to predict the distribution range of the suitable area for C. coreana under the current (1971–2000) and four future scenarios. The buffer screening method screened data from 41 distribution points that could be used for modeling. The main factors affecting the distribution of C. coreana were precipitation in the driest month (Bio14), precipitation in the warmest quarter (Bio18), precipitation in the coldest quarter (Bio19), the standard deviation of seasonal variation of temperature (Bio4), minimum temperature in the coldest month (Bio6), and average temperature in the coldest quarter (Bio11). The feature class (FC) after the kuenm package optimization was a Q-quadratic T-threshold combination, and the regularization multiplier (RM) was 0.8. The suitable areas for C. coreana under the current climate model were mainly distributed in central China, and the highly suitable areas were distributed in southern Shaanxi, southwestern Henan, and northwestern Hubei. The lowest temperature in the coldest month (Bio6), the average temperature in the coldest quarter (Bio11), and the precipitation in the warmest quarter (Bio18) all had good predictive ability. In future climate scenarios, the boundary of the suitable area for C. coreana in China is expected to shift northward, and thus, most of the future climate scenarios would shift northward.
Information Collection and Personalized Service Strategy of Monopoly under Consumer Misrepresentation
Authors: Zhong Mingyue , Cheng Yan , Mei Shu-e , Zhong Weijun
Published: June 2024

To decrease privacy risks, consumers may choose to misrepresent themselves when they are asked to offer personal information. Using a game theoretic model, this study examines the impact of consumer misrepresentation on both a monopolistic firm and consumers. The results show that consumer misrepresentation may benefit the firm, but hurt consumers under certain conditions. In addition, we find that when the unit cost of personalized service is low, consumer misrepresentation may encourage the firm to provide a higher personalized service level. Moreover, when consumers misrepresent themselves and the firm only covers part of the market, a greater unit value of consumer private information will reduce the firm’s profit, while a greater unit cost of personalized service will increase the firm’s profit. The analysis reported here provides important insights regarding the application of consumer information in online personalized marketing and consumer privacy protection.
Coordination of Online Shopping Supply Chain Considering Fresh Product Preservation Efforts and Cargo Damage Costs
Authors: Ren Haiping , Hu Yingxin
Published: June 2024

To reduce the losses caused by insufficient preservation efforts during transportation, the preservation effort level has been the focus of research. In the fierce competition of online sales, it is particularly important to reduce the cost of damaged goods by improving the level of preservation efforts. Therefore, according to Stackelberg game theory, this article establishes five decision-making models and incorporates the damage rate and preservation effort level into the research. Finally, this article coordinates the online shipping supply chain (SC) through a joint contract. After comparing and analyzing the model results, research has found that: (1) in centralized model, the level of preservation effort reaches its optimal level and the system benefit is maximized; (2) under third-party logistics (TPL) leading decision-making, the different bearers of cargo damage costs will not affect the profits of both parties and the system; (3) among the four decentralized models, the level of preservation efforts and system profit are highest when the decision is led by online store and TPL bears the cost of damaged goods; and (4) under a given sharing ratio, when the logistics service quotation satisfies a certain range of condition, the online shopping SC can achieve Pareto improvement. This paper studies the differences and reasons for decision models in the supply and demand relationship between online stores and TPL, which provides fresh product e-commerce decision-makers with a theoretical basis.
The Utilization of Chicken Egg White Waste-Modified Nanofiber Membrane for Anionic Dye Removal in Batch and Flow Systems: Comprehensive Investigations into Equilibrium, Kinetics, and Breakthrough Curve
Authors: Chen Yun-Rou , Thanh Dinh Thi Hong , Tran Quynh Thi Phuong , Liu Bing-Lan , Srinophakun Penjit , Chiu Chen-Yaw , Chen Kuei-Hsiang , Chang Yu-Kaung
Published: June 2024

This study investigated the use of chicken egg white (CEW) waste immobilized on weak acidic nanofiber membranes for removing the anionic acid orange 7 (AO7) dye in batch and continuous flow modes. Different experiments were conducted to evaluate the effectiveness of CEW-modified nanofiber membranes for AO7 removal, focusing on CEW immobilization conditions, adsorption kinetics, and thermodynamics. The CEW-modified nanofiber membrane (namely NM-COOH-CEW) exhibited a maximum AO7 adsorption capacity of 589.11 mg/g within approximately 30 min. The Freundlich isotherm model best represented the equilibrium adsorption data, while the adsorption kinetics followed a pseudo-second-order rate model. Breakthrough curve analysis using the Thomas model and the bed depth service time (BDST) model showed that the BDST model accurately described the curve, with an error percentage under 5%. To investigate AO7 elution efficiency, different concentrations of organic solvents or salts were tested as eluents. The NM-COOH-CEW nanofiber membrane exhibited promising performance as an effective adsorbent for removing AO7 dye from contaminated water.
Investigating the Hepatoprotective Properties of Mulberry Leaf Flavonoids against Oxidative Stress in HepG2 Cells
Authors: Zheng Qinhua , Feng Ke , Zhong Wenting , Tan Weijian , Rengaowa Sa , Hu Wenzhong
Published: June 2024

Oxidative stress significantly contributes to ageing and disease, with antioxidants holding promise in mitigating its effects. Functional foods rich in flavonoids offer a potential strategy to mitigate oxidative damage by free radicals. We investigated the protective effects of mulberry leaf flavonoids (MLF) against H2O2-induced oxidative damage in HepG2 cells. It assessed the inhibitory effect of MLF (62.5–500 μg/mL) on H2O2-induced oxidative damage by analyzing cellular morphology and oxidative stress markers, including ROS production, mitochondrial membrane potential, antioxidant enzyme levels, MDA, and apoptosis-related proteins. The results demonstrated that MLF prevented spiny cell formation triggered by 750 μM H2O2 and significantly reduced ROS levels, restored mitochondrial membrane potential, decreased lactate dehydrogenase and alanine transaminase leakage, and reduced MDA content induced by H2O2. MLF also modulated antioxidant enzymes and attenuated oxidative damage to HepG2 cell DNA, as confirmed by staining techniques. These findings indicate the potential of MLF as a hepatoprotective agent against oxidative damage in HepG2 cells.
Optimization of Flavonoid Extraction from Abelmoschus manihot Flowers Using Ultrasonic Techniques: Predictive Modeling through Response Surface Methodology and Deep Neural Network and Biological Activity Assessment
Authors: Sun Zenghong , Liu Wenhui , Zhang Sha , Tian Shuge , Aikemu Ainiwaer
Published: June 2024

Understanding the optimal extraction methods for flavonoids from Abelmoschus manihot flowers (AMF) is crucial for unlocking their potential benefits. This study aimed to optimize the efficiency of flavonoid extraction from AMF. After comparing extraction methods, the ultrasonic cell crusher demonstrated superior performance over conventional techniques. Four key factors—solid-to-liquid ratio (1:10 to 1:50 g·mL−1), ethanol concentration (55% to 95%), ultrasonic time (10 to 50 min), and ultrasonic power (5% to 25% of 900 W)—were investigated and normalized using the entropy weight method. This led to a comprehensive evaluation (CE). Optimization of extraction conditions for the ultrasonic cell crusher was achieved through response surface methodology and a deep neural network model, resulting in optimal parameters: ethanol volume fraction of 66%, solid-to-liquid ratio of 1:21 g/mL, extraction efficiency of 9%, and extraction duration of 35 min, yielding a CE value of 23.14 (RSD < 1%). Additionally, the inhibitory effects of the optimized extracts against Streptococcus mutans (S. mutans) were assessed. The results revealed that AMF extract (AMFE) exhibits inhibitory effects on S. mutans, with concomitant inhibition of sucrase and lactate dehydrogenase (LDH). The MIC of AMFE against planktonic S. mutans is 3 mg/mL, with an MBC of 6 mg/mL. Within the concentration range of 1/8 MIC to 2 MIC of AMFE, the activities of sucrase and LDH decreased by 318.934 U/mg prot and 61.844 U/mg prot, respectively. The antioxidant activity of AMFE was assessed using the potassium ferricyanide reduction and phosphomolybdenum methods. Additionally, the effect of AMFE on DPPH, ABTS, and ·OH free radical scavenging abilities was determined. The concentrations at which AMFE exhibited over 90% scavenging rate for ABTS and DPPH free radicals were found to be 0.125 mg/mL and 2 mg/mL, respectively.
Comparative Analysis of Structural and Functional Properties of Dietary Fiber from Four Grape Varieties
Authors: Chang Yingying , An Ran , Sun Sijie , Hou Min , Han Fuliang , Song Shiren
Published: June 2024

Muscadine grapes are characterized by their large and abundant seeds and hard and thick skins that contain significant amounts of dietary fiber (DF). The current study investigated the chemical constituents, molecular architecture, and physicochemical attributes of DF derived from Muscadine grapes (Granny Val and Alachua) and compared them with those derived from Shine Muscat and Kyoho. Using a combined enzymatic method, the total dietary fiber (TDF) was extracted and divided into two parts: soluble dietary fiber (SDF) and insoluble dietary fiber (IDF). TDF (mainly IDF, with a small fraction of SDF) was dominated by cellulose, followed by pectin and hemicellulose. In addition, Granny Val and Alachua had a significantly higher abundance of TDF and IDF compared with Shine Muscat and Kyoho. Moreover, Shine Muscat had significantly the highest abundance of SDF among the four grape varieties. Of note, IDF from Granny Val and Alachua exhibited a complex and dense texture on its surface, and notably outperformed Shine Muscat and Kyoho in terms of cholesterol, fatty acid, heavy metal adsorption, and antioxidant activity. Collectively, Muscadine grapes, i.e., Granny Val and Alachua in the current study, possessed elevated DF levels (predominantly IDF), and their enhanced bioactivity underscored their potential as a potential food ingredient for further use.
Trichostatin A Promotes Cytotoxicity of Cisplatin, as Evidenced by Enhanced Apoptosis/Cell Death Markers
Authors: Zhou Yang , Luo Qun , Zeng Fangang , Liu Xingkai , Han Juanjuan , Gu Liangzhen , Tian Xiao , Zhang Yanyan , Zhao Yao , Wang Fuyi
Published: June 2024

Trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, promotes the cytotoxicity of the genotoxic anticancer drug cisplatin, yet the underlying mechanism remains poorly understood. Herein, we revealed that TSA at a low concentration (1 μM) promoted the cisplatin-induced activation of caspase-3/6, which, in turn, increased the level of cleaved PARP1 and degraded lamin A&C, leading to more cisplatin-induced apoptosis and G2/M phase arrest of A549 cancer cells. Both ICP-MS and ToF-SIMS measurements demonstrated a significant increase in DNA-bound platinum in A549 cells in the presence of TSA, which was attributable to TSA-induced increase in the accessibility of genomic DNA to cisplatin attacking. The global quantitative proteomics results further showed that in the presence of TSA, cisplatin activated INF signaling to upregulate STAT1 and SAMHD1 to increase cisplatin sensitivity and downregulated ICAM1 and CD44 to reduce cell migration, synergistically promoting cisplatin cytotoxicity. Furthermore, in the presence of TSA, cisplatin downregulated TFAM and SLC3A2 to enhance cisplatin-induced ferroptosis, also contributing to the promotion of cisplatin cytotoxicity. Importantly, our posttranslational modification data indicated that acetylation at H4K8 played a dominant role in promoting cisplatin cytotoxicity. These findings provide novel insights into better understanding the principle of combining chemotherapy of genotoxic drugs and HDAC inhibitors for the treatment of cancers.
Development of In-Needle SPME Devices for Microextraction Applied to the Quantification of Pesticides in Agricultural Water
Authors: Alshehri Ahmed Ali , Hammami Bechir , Alshehri Mohammed Mousa , Aouak Taieb , Hakami Rabab A. , Badjah Hadj Ahmed Ahmed Yacine
Published: June 2024

The chemical industry explosion in the 20th century has led to increased environmental pollution, affecting fauna, flora, and waterways. These substances alter water’s taste, color, and smell, making it unfit for consumption or toxic. Agricultural water networks face threats from pollution before and after treatment. Some chemical contaminants, like pesticides, are embedded in natural biogeochemical cycles. In this study, we developed a simple and low-cost procedure for the fabrication of needles coated with polydimethylsiloxane (PDMS) as an efficient sorbent for the microextraction of organic pollutant traces from water. The prepared needles were used as an alternative for commercial solid-phase micro-extraction (SPME) devices in analytical chemistry. The PDMS polymeric phase was characterized by Fourier-transform infrared spectroscopy (FT-IR), thermogravimetry (TGA), and scanning electron microscopy (SEM). The PDMS-coated needles were used for extraction of thirteen pesticides by direct-immersion solid-phase microextraction (DI-SPME) from contaminated waters, followed by determination with gas chromatography-mass spectrometry (GC-MS). The developed analytical method showed limits of detection (LODs) between 0.3 and 2.5 ng mL−1 and RSDs in the range of 0.8–12.2%. The homemade needles were applied for the extraction of pesticides in surface and ground aqueous samples collected from an agricultural area. Several target pesticides were identified and quantified in the investigated water samples.
Non-Isocyanate Urethane Acrylate Derived from Isophorone Diamine: Synthesis, Characterization and Its Application in 3D Printing
Authors: Zhang Xinqi , Zan Xinxin , Yin Jiangdi , Wang Jiaxi
Published: June 2024

In this paper, urethane-based acrylates (UA) were prepared via an environmentally friendly non-isocyanate route. Isophorone diamine (IPDA) reacted with ethylene carbonate (EC), producing carbamate containing amine and hydroxyl groups, which further reacted with neopentyl glycol diacrylate (NPGDA) by aza Michael addition, forming UA. The structures of the obtained intermediates and UA were characterized by 1H NMR and electrospray ionization high-resolution mass spectrometry (ESI-HRMS). The photopolymerization kinetics of UA were investigated by infrared spectroscopy. The composite with obtained UA can be UV cured quickly to form a transparent film with a tensile strength of 21 MPa and elongation at break of 16%. After UV curing, the mono-functional urethane acrylate was copolymerized into the cross-linked network in the form of side chains. The hydroxyl and carbamate bonds on the side chains have high mobility, which make them easy to form stronger dynamic hydrogen bonds during the tensile process, giving the material a higher tensile strength and elongation at break. Therefore, the hydrogen bonding model of a cross-linked network is proposed. The composite with UA can be 3D printed into models.
Effect of MnO2 Nanoparticles Stabilized with Cocamidopropyl Betaine on Germination and Development of Pea (Pisum sativum L.) Seedlings
Authors: Nagdalian Andrey , Blinov Andrey , Gvozdenko Alexey , Golik Alexey , Rekhman Zafar , Rzhepakovsky Igor , Kolesnikov Roman , Avanesyan Svetlana , Blinova Anastasiya , Pirogov Maxim , Leontev Pavel , Askerova Alina , Tsykin Evgeniy , Shariati Mohammad Ali
Published: June 2024

This study aimed to synthesize, characterize, and evaluate the effect of cocamidopropyl betaine-stabilized MnO2 nanoparticles (NPs) on the germination and development of pea seedlings. The synthesized NPs manifested as aggregates ranging from 50–600 nm, comprising spherical particles sized between 19 to 50 nm. These particles exhibited partial crystallization, indicated by peaks at 2θ = 25.37, 37.62, 41.18, 49.41, 61.45, and 65.79°, characteristic of MnO2 with a tetragonal crystal lattice with a I4/m spatial group. Quantum chemical modelling showed that the stabilization process of MnO2 NPs with cocamidopropyl betaine is energetically advantageous (∆E > 1299.000 kcal/mol) and chemically stable, as confirmed by the positive chemical hardness values (0.023 ≤ η ≤ 0.053 eV). It was revealed that the interaction between the MnO2 molecule and cocamidopropyl betaine, facilitated by a secondary amino group (NH), is the most probable scenario. This ascertain is supported by the values of the difference in total energy (∆E = 1299.519 kcal/mol) and chemical hardness (η = 0.053 eV). These findings were further confirmed using FTIR spectroscopy. The effect of MnO2 NPs at various concentrations on the germination of pea seeds was found to be nonlinear and ambiguous. The investigation revealed that MnO2 NPs at a concentration of 0.1 mg/L resulted in the highest germination energy (91.25%), germinability (95.60%), and lengths of roots and seedlings among all experimental samples. However, an increase in the concentration of preparation led to a slight growth suppression (1–10 mg/L) and the pronounced inhibition of seedling and root development (100 mg/L). The analysis of antioxidant indicators and phytochemicals in pea seedlings indicated that only 100 mg/L MnO2 NPs have a negative effect on the content of soluble sugars, chlorophyll a/b, carotenoids, and phenols. Conversely, lower concentrations showed a stimulating effect on photosynthesis indicators. Nevertheless, MnO2 NPs at all concentrations generally decreased the antioxidant potential of pea seedlings, except for the ABTS parameter. Pea seedlings showed a notable capacity to absorb Mn, reaching levels of 586.5 μg/L at 10 mg/L and 892.6 μg/L at 100 mg/L MnO2 NPs, surpassing the toxic level for peas according to scientific literature. However, the most important result was the observed growth-stimulating activity at 0.1 mg/L MnO2 NPs stabilized with cocamidopropyl betaine, suggesting a promising avenue for further research.
Influence of Hole Transport Layers on Buried Interface in Wide-Bandgap Perovskite Phase Segregation
Authors: Cao Fangfang , Du Liming , Jiang Yongjie , Gou Yangyang , Liu Xirui , Wu Haodong , Zhang Junchuan , Qiu Zhiheng , Li Can , Ye Jichun , Li Zhen , Xiao Chuanxiao
Published: June 2024

Light-induced phase segregation, particularly when incorporating bromine to widen the bandgap, presents significant challenges to the stability and commercialization of perovskite solar cells. This study explores the influence of hole transport layers, specifically poly[bis(4-phenyl)(2,4,6-trimethylphenyl)amine (PTAA) and [4-(3,6-dimethyl-9H-carbazol-9-yl)butyl]phosphonic acid (Me-4PACz), on the dynamics of phase segregation. Through detailed characterization of the buried interface, we demonstrate that Me-4PACz enhances perovskite photostability, surpassing the performance of PTAA. Nanoscale analyses using in situ Kelvin probe force microscopy and quantitative nanomechanical mapping techniques elucidate defect distribution at the buried interface during phase segregation, highlighting the critical role of substrate wettability in perovskite growth and interface integrity. The integration of these characterization techniques provides a thorough understanding of the impact of the buried bottom interface on perovskite growth and phase segregation.
Encapsulation and Evolution of Polyynes Inside Single-Walled Carbon Nanotubes
Authors: Tang Kunpeng , Li Yinong , Chen Yingzhi , Cui Weili , Lin Zhiwei , Zhang Yifan , Shi Lei
Published: June 2024

Polyyne is an sp-hybridized linear carbon chain (LCC) with alternating single and triple carbon–carbon bonds. Polyyne is very reactive; thus, its structure can be easily damaged through a cross-linking reaction between the molecules. The longer the polyyne is, the more unstable it becomes. Therefore, it is difficult to directly synthesize long polyynes in a solvent. The encapsulation of polyynes inside carbon nanotubes not only stabilizes the molecules to avoid cross-linking reactions, but also allows a restriction reaction to occur solely at the ends of the polyynes, resulting in long LCCs. Here, by controlling the diameter of single-walled carbon nanotubes (SWCNTs), polyynes were filled with high yield below room temperature. Subsequent annealing of the filled samples promoted the reaction between the polyynes, leading to the formation of long LCCs. More importantly, single chiral (6,5) SWCNTs with high purity were used for the successful encapsulation of polyynes for the first time, and LCCs were synthesized by coalescing the polyynes in the (6,5) SWCNTs. This method holds promise for further exploration of the synthesis of property-tailored LCCs through encapsulation inside different chiral SWCNTs.
Preparation of Few-Layered MoS2 by One-Pot Hydrothermal Method for High Supercapacitor Performance
Authors: Jia Qingling , Wang Qi , Meng Lingshuai , Zhao Yujie , Xu Jing , Sun Meng , Li Zijian , Li Han , Chen Huiyu , Zhang Yongxing
Published: June 2024

Molybdenum disulfide (MoS2), a typical layered material, has important applications in various fields, such as optoelectronics, catalysis, electronic devices, sensors, and supercapacitors. Extensive research has been carried out on few-layered MoS2 in the field of electrochemistry due to its large specific surface area, abundant active sites and short electron transport path. However, the preparation of few-layered MoS2 is a significant challenge. This work presents a simple one-pot hydrothermal method for synthesizing few-layered MoS2. Furthermore, it investigates the exfoliation effect of different amounts of sodium borohydride (NaBH4) as a stripping agent on the layer number of MoS2. Na+ ions, as alkali metal ions, can intercalate between layers to achieve the purpose of exfoliating MoS2. Additionally, NaBH4 exhibits reducibility, which can effectively promote the formation of the metallic phase of MoS2. Few-layered MoS2, as an electrode for supercapacitor, possesses a wide potential window of 0.9 V, and a high specific capacitance of 150 F g−1 at 1 A g−1. This work provides a facile method to prepare few-layered two-dimensional materials for high electrochemical performance.
Nutraceutical Features of the Phycobiliprotein C-Phycocyanin: Evidence from Arthrospira platensis (Spirulina)
Authors: Citi Valentina , Torre Serenella , Flori Lorenzo , Usai Luca , Aktay Nazlim , Dunford Nurhan Turgut , Lutzu Giovanni Antonio , Nieri Paola
Published: June 2024

Arthrospira platensis, commonly known as Spirulina, is a photosynthetic filamentous cyanobacterium (blue–green microalga) that has been utilized as a food source since ancient times. More recently, it has gained significant popularity as a dietary supplement due to its rich content of micro- and macro-nutrients. Of particular interest is a water soluble phycobiliprotein derived from Spirulina known as phycocyanin C (C-PC), which stands out as the most abundant protein in this cyanobacterium. C-PC is a fluorescent protein, with its chromophore represented by the tetrapyrrole molecule phycocyanobilin B (PCB-B). While C-PC is commonly employed in food for its coloring properties, it also serves as the molecular basis for numerous nutraceutical features associated with Spirulina. Indeed, the comprehensive C-PC, and to some extent, the isolated PCB-B, has been linked to various health-promoting effects. These benefits encompass conditions triggered by oxidative stress, inflammation, and other pathological conditions. The present review focuses on the bio-pharmacological properties of these molecules, positioning them as promising agents for potential new applications in the expanding nutraceutical market.
Effect of 2 Years of Monthly Calcifediol Administration in Postmenopausal Women with Vitamin D Insufficiency
Authors: Occhiuto Marco , Pepe Jessica , Colangelo Luciano , Lucarelli Marco , Angeloni Antonio , Nieddu Luciano , De Martino Viviana , Minisola Salvatore , Cipriani Cristiana
Published: June 2024

Background: We assessed the long-term (24 months) efficacy and safety of monthly calcifediol (0.266 mg) in the correction and maintenance of total 25(OH)D levels in postmenopausal women with basal values <30 ng/mL. Methods: We initially enrolled 45 consecutive patients during the period September 2019–September 2020. After an initial visit, patients were instructed to return at 3, 6, 9, 12 and 24 months for measuring serum total 25(OH)D, ionised calcium, creatinine and isoenzyme of alkaline phosphatase (bALP). Here, we report only the per-protocol analysis, because the COVID-19 pandemic precluded adherence to the scheduled visits for some patients. Results: The patients’ mean age was 62.4 ± 9.0 years. Mean basal 25(OH)D levels were 20.5 ± 5.3 ng/mL. There was a continuous increase of mean 25(OH)D values (p for trend < 0.001). However, mean values at month 24 (36.7 ± 15.9) were not significantly different in respect to values at month 12 (41.2 ± 11.18). At 24 months, only 1 out 19 patients had a value <20 ng/mL. There was a significant decrease with time of mean values of bALP (p < 0.0216), with no significant changes between 12 and 24 months. No significant changes were observed as far as ionised calcium or creatinine were concerned. Conclusions: The long-term administration of calcifediol maintains stable and sustained 25(OH)D concentrations, with no safety concerns.