bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanisms of Ohmic Contact Formation of Ti/Al-Based Metal Stacks on p-Doped 4H-SiC
Authors: Kocher Matthias , Rommel Mathias , Michałowski Paweł Piotr , Erlbacher Tobias
Published: December 2021

Ohmic contacts on p-doped 4H-SiC are essential for the fabrication of a wide range of power electron devices. Despite the fact that Ti/Al based ohmic contacts are routinely used for ohmic contacts on p-doped 4H-SiC, the underlying contact formation mechanisms are still not fully understood. TLM structures were fabricated, measured and analyzed to get a better understanding of the formation mechanism. SIMS analyses at the Ti3SiC2-SiC interface have shown a significant increase of the surface near Al concentration. By using numerical simulation it is shown that this additional surface near Al concentration is essential for the ohmic contact formation.
Alterations to Titanium Surface Depending on the Fluorides and Abrasives in Toothpaste
Authors: Shuto Takahiro , Mine Yuichi , Makihira Seicho , Nikawa Hiroki , Wachi Takanori , Kakimoto Kazutoshi
Published: December 2021

Fluoride and abrasives in toothpastes may cause corrosion and deterioration of the titanium used for implants and other prostheses. The purpose of this study was to investigate how the presence or absence and types of fluoride and abrasives affected the titanium surface texture. Brushing with toothpastes was performed on pure-titanium discs using an abrasive testing machine. Unprocessed titanium discs without brushing were used as control samples. Surface roughness, color, and gloss of titanium were measured and the differences compared with the control were analyzed. Additionally, titanium surfaces and abrasives in toothpastes were observed using a scanning electron microscope to compare the surface texture of each sample. Some toothpastes (abrasive+) significantly increased the difference in surface roughness, color, and gloss, compared with ultrapure water. Toothpaste (fluoride+/abrasive+) that had many polygonal abrasive particles led to the largest color differences and exhibited notable scratches and a larger number of contaminant- or corrosion-like black spots. In contrast, brushing with toothpaste without fluoride or abrasives (fluoride−/abrasive−) caused little change to the titanium surface. These results suggest that both fluoride and abrasives in toothpaste used for brushing may be factors that affect surface texture and corrosion resistance of titanium.
Simulation of Depth of Wear of Eco-Friendly Concrete Using Machine Learning Based Computational Approaches
Authors: Khan Mohsin Ali , Farooq Furqan , Javed Mohammad Faisal , Zafar Adeel , Ostrowski Krzysztof Adam , Aslam Fahid , Malazdrewicz Seweryn , Maślak Mariusz
Published: December 2021

To avoid time-consuming, costly, and laborious experimental tests that require skilled personnel, an effort has been made to formulate the depth of wear of fly-ash concrete using a comparative study of machine learning techniques, namely random forest regression (RFR) and gene expression programming (GEP). A widespread database comprising 216 experimental records was constructed from available research. The database includes depth of wear as a response parameter and nine different explanatory variables, i.e., cement content, fly ash, water content, fine and coarse aggregate, plasticizer, air-entraining agent, age of concrete, and time of testing. The performance of the models was judged via statistical metrics. The GEP model gives better performance with R2 and ρ equals 0.9667 and 0.0501 respectively and meet with the external validation criterion suggested in the previous literature. The k-fold cross-validation also verifies the accurateness of the model by evaluating R2, RSE, MAE, and RMSE. The sensitivity analysis of GEP equation indicated that the time of testing is the influential parameter. The results of this research can help the designers, practitioners, and researchers to quickly estimate the depth of wear of fly-ash concrete thus shortening its ecological susceptibilities that push to sustainable and faster construction from the viewpoint of environmentally friendly waste management.
Estimation of Minimum Uncut Chip Thickness during Precision and Micro-Machining Processes of Various Materials—A Critical Review
Authors: Wojciechowski Szymon
Published: December 2021

Evaluation of the phenomena characterizing the chip decohesion process during cutting is still a current problem in relation to precision, ultra-precision, and micro-machining processes of construction materials. The reliable estimation of minimum uncut chip thickness is an especially challenging task since it directly affects the machining process dynamics and formation of a surface topography. Therefore, in this work a critical review of the recent studies concerning the determination of minimum uncut chip thickness during precision, ultra-precision, and micro-cutting is presented. The first part of paper covers a characterization of the precision, ultra-precision, and micro-cutting processes. In the second part, the analytical, experimental, and numerical methods for minimum uncut chip thickness estimation are presented in detail. Finally, a summary of the research results for minimum uncut chip thickness estimation is presented, together with conclusions and a determination of further research directions.
Enhancement of the Water-Lubricated Tribological Properties of Hybrid PTFE/Nomex Fabric Laminate Composite via Epoxy Resin and Graphite Filler
Authors: Liu Ying , Gao Gengyuan , Jiang Dan , Yin Zhongwei
Published: December 2021

This paper studied a hybrid polytetrafluoroethylene (PTFE)/Nomex fabric composite with lower friction coefficient (COF) and high underwater wear resistance. A pin-on-disk tribometer was used to test tribological properties under different applied loads and rotation speeds. The wear surface, transfer film and cross-section were analyzed by scanning electron microscope (SEM) and optical microscope. The results showed enhanced underwater tribological properties because of excellent self-lubricating properties of PTFE fibers and a good lubricating effect and load-carrying capacity of graphite fillers. Improved underwater mechanical strength was connected to the high strength of epoxy resin and high bonding force between Nomex and epoxy resin.
Biomedical Alloys and Physical Surface Modifications: A Mini-Review
Authors: Yan Xinxin , Cao Wei , Li Haohuan
Published: December 2021

Biomedical alloys are essential parts of modern biomedical applications. However, they cannot satisfy the increasing requirements for large-scale production owing to the degradation of metals. Physical surface modification could be an effective way to enhance their biofunctionality. The main goal of this review is to emphasize the importance of the physical surface modification of biomedical alloys. In this review, we compare the properties of several common biomedical alloys, including stainless steel, Co–Cr, and Ti alloys. Then, we introduce the principle and applications of some popular physical surface modifications, such as thermal spraying, glow discharge plasma, ion implantation, ultrasonic nanocrystal surface modification, and physical vapor deposition. The importance of physical surface modifications in improving the biofunctionality of biomedical alloys is revealed. Future studies could focus on the development of novel coating materials and the integration of various approaches.
Uniaxially Strained Graphene: Structural Characteristics and G-Mode Splitting
Authors: Kalosakas George , Lathiotakis Nektarios N. , Papagelis Konstantinos
Published: December 2021

The potential use of graphene in various strain engineering applications requires an accurate characterization of its properties when the material is under different mechanical loads. In this work, we present the strain dependence of the geometrical characteristics at the atomic level and the Raman active G-band evolution in a uniaxially strained graphene monolayer, using density functional theory methods as well as molecular dynamics atomistic simulations for strains that extend up to the structural failure. The bond length and bond angle variations with strain, applied either along the zigzag or along the armchair direction, are discussed and analytical relations describing this dependence are provided. The G-mode splitting with strain, as obtained by first principles’ methods, is also presented. While for small strains, up to around 1%, the G-band splitting is symmetrical in the two perpendicular directions of tension considered here, this is no longer the case for larger values of strains where the splitting appears to be larger for strains along the zigzag direction. Further, a crossing is observed between the lower frequency split G-mode component and the out-of-plane optical mode at the Γ point for large uniaxial strains (>20%) along the zigzag direction.
Mechanical Behaviour of Aluminium-Timber Composite Connections with Screws and Toothed Plates
Authors: Chybiński Marcin , Polus Łukasz
Published: December 2021

This paper presents an investigation of the load-slip behaviour of aluminium-timber composite connections. Toothed plates with bolts are often used for connecting timber structural members with steel structural members. In this paper, toothed plates (C2-50/M10G, C2-50/M12G or C11-50/M12) have been used as reinforcement in aluminium-timber screwed connections for the first time. The push-out test specimens consisted of laminated veneer lumber slabs, aluminium alloy beams, and hexagon head wood screws (10 mm × 80 mm and 12 mm × 80 mm). Of the specimens, 12 additionally had toothed plates as reinforcement, while 8 had no reinforcement. The load carrying-capacity, the mode of failure and the load-slip response of the strengthened and non-strengthened screwed connections were investigated. The use of toothed plate connectors was found to be effective in increasing the strength of aluminium-timber composite connections and ineffective in improving their stiffness. The examined stiffness and strength of the connections can be used in the design and numerical modelling of aluminium-timber composite beams with reinforced screwed connections.
Abrasion and Erosion Resistance of Cermets: A Review
Authors: Kübarsepp Jakob , Juhani Kristjan , Tarraste Marek
Published: December 2021

WC-based hardmetals are employed widely as wear-resistant ceramic–metal composites for tools and wear parts. Raw materials supply, environmental concerns and some limitations of hardmetals have directed efforts toward development of alternative wear-resistant composites–cermets. We present a current state of knowledge in the field of ceramic-rich (≥50 vol%) cermets behavior in abrasion and erosion conditions, which are the dominant types of wear in many industrial applications. Distinction is made between two-body and three-body abrasion, solid-particle erosion, and slurry erosion. Cermets, in particular TiC-, Ti(C,N)- and Cr3C2-based composites and hardmetals, are compared for their abrasive and erosive wear performance and mechanism. The review enabled formulation of tribological conditions in which cermets may be comparable or have potential to outperform WC-Co hardmetals. Hardmetals, in general, outperform cermets in abrasion and solid-particle erosion at room and moderate temperatures. However, cermets demonstrate their potential mainly in severe conditions—at elevated temperatures and corrosive (oxidation, electrochemical corrosion) environments.
Study on Improvement of Lightning Damage Detection Model for Wind Turbine Blade
Authors: Matsui Takuto , Yamamoto Kazuo , Ogata Jun
Published: December 2021

There have been many reports of damage to wind turbine blades caused by lightning strikes in Japan. In some of these cases, the blades struck by lightning continue to rotate, causing more serious secondary damage. To prevent such accidents, it is a requirement that a lightning detection system is installed on the wind turbine in areas where winter lightning occurs in Japan. This immediately stops the wind turbine if the system detects a lightning strike. Normally, these wind turbines are restarted after confirming soundness of the blade through visual inspection. However, it is often difficult to confirm the soundness of the blade visually for reasons such as bad weather. This process prolongs the time taken to restart, and it is one of the causes that reduces the availability of the wind turbines. In this research, we constructed a damage detection model for wind turbine blades using machine learning based on SCADA system data and, thereby, considered whether the technology automatically confirms the soundness of wind turbine blades.
Unmanned Aerial Vehicles Motion Control with Fuzzy Tuning of Cascaded-PID Gains
Authors: Andrade Fabio A. A. , Guedes Ihannah P. , Carvalho Guilherme F. , Zachi Alessandro R. L. , Haddad Diego B. , Almeida Luciana F. , de Melo Aurélio G. , Pinto Milena F.
Published: December 2021

One of the main challenges of maneuvering an Unmanned Aerial Vehicle (UAV) to keep a stabilized flight is dealing with its fast and highly coupled nonlinear dynamics. There are several solutions in the literature, but most of them require fine-tuning of the parameters. In order to avoid the exhaustive tuning procedures, this work employs a Fuzzy Logic strategy for online tuning of the PID gains of the UAV motion controller. A Cascaded-PID scheme is proposed, in which velocity commands are calculated and sent to the flight control unit from a given target desired position (waypoint). Therefore, the flight control unit is responsible for the lower control loop. The main advantage of the proposed method is that it can be applied to any UAV without the need of its formal mathematical model. Robot Operating System (ROS) is used to integrate the proposed system and the flight control unit. The solution was evaluated through flight tests and simulations, which were conducted using Unreal Engine 4 with the Microsoft AirSim plugin. In the simulations, the proposed method is compared with the traditional Ziegler-Nichols tuning method, another Fuzzy Logic approach, and the ArduPilot built-in PID controller. The simulation results show that the proposed method, compared to the ArduPilot controller, drives the UAV to reach the desired setpoint faster. When compared to Ziegler-Nichols and another different Fuzzy Logic approach, the proposed method demonstrates to provide a faster accommodation and yield smaller errors amplitudes.
Comparison of Hydroxypropylcellulose and Hot-Melt Extrudable Hypromellose in Twin-Screw Melt Granulation of Metformin Hydrochloride: Effect of Rheological Properties of Polymer on Melt Granulation and Granule Properties
Authors: Batra Amol , Yang Fengyuan , Kogan Michael , Sosnowik Anthony , Usher Courtney , Oldham Eugene W. , Chen Ningyi , Lawal Kamaru , Bi Yunxia , Dürig Thomas
Published: December 2021

High-molecular-weight hypromellose (HPMC) and hydroxypropyl cellulose (HPC) are widely known, extended-release polymers. Conventional high-molecular-weight HPMCs are preferred in extended-release applications but not widely used in twin-screw melt granulation due to processability difficulties at low operating temperatures and potential drug degradation if high processing temperatures are used. Conversely, high-molecular-weight grade HPC (Klucel®) can be used in melt granulation processes. The purpose of this study was to evaluate the processability and dissolution behavior of HPC GXF ((Klucel® GXF) and a recently introduced type of hot-melt extrudable HPMC (Affinisol®) in extended-release metformin hydrochloride formulations using twin-screw melt granulation. Powder blends were prepared with 75% w/w metformin HCl and 25% w/w polymeric binder. Blends were granulated at processing temperatures of 160, 140, 120 and 100 °C. HPMC HME 4M (Affinisol® 4M) provided a fine powder, indicating minimum granulation at processing temperatures lower than 160 °C, and the tablets obtained with these granules capped during tableting. In contrast, acceptable tablets could be obtained with HPC GXF at all processing temperatures. Rheological studies including capillary rheometry to measure steady shear rate viscosity, and rotational rheometry to obtain time and temperature superposition data, showed that HPC GXF had a greater thermoplasticity than HPMC HME 4M, which made granulation possible with HPC GXF at low temperatures. Tablets compressed with granules obtained at 160 °C with both binders showed comparable dissolution profiles. High-molecular-weight HPC GXF provided a better processability at low temperatures and adequate tablet strength for the melt granulation of metformin HCl.
Tripled Fixed Points and Existence Study to a Tripled Impulsive Fractional Differential System via Measures of Noncompactness
Authors: Etemad Sina , Matar Mohammed M. , Ragusa Maria Alessandra , Rezapour Shahram
Published: December 2021

In this paper, a tripled fractional differential system is introduced as three associated impulsive equations. The existence investigation of the solution is based on contraction principle and measures of noncompactness in terms of tripled fixed point and modulus of continuity. Our results are valid for both Kuratowski and Hausdorff measures of noncompactness. As an application, we apply the obtained results to a control problem.
A VMD–CISSA–LSSVM Based Electricity Load Forecasting Model
Authors: Wang Guijuan , Wang Xinheng , Wang Zuoxun , Ma Chunrui , Song Zengxu
Published: December 2021

Accurate power load forecasting has an important impact on power systems. In order to improve the load forecasting accuracy, a new load forecasting model, VMD–CISSA–LSSVM, is proposed. The model combines the variational modal decomposition (VMD) data preprocessing method, the sparrow search algorithm (SSA) and the least squares support vector machine (LSSVM) model. A multi-strategy improved chaotic sparrow search algorithm (CISSA) is proposed to address the shortcomings of the SSA algorithm, which is prone to local optima and a slow convergence. The initial population is generated using an improved tent chaotic mapping to enhance the quality of the initial individuals and population diversity. Second, a random following strategy is used to optimize the position update process of the followers in the sparrow search algorithm, balancing the local exploitation performance and global search capability of the algorithm. Finally, the Levy flight strategy is used to expand the search range and local search capability. The results of the benchmark test function show that the CISSA algorithm has a better search accuracy and convergence performance. The volatility of the original load sequence is reduced by using VMD. The optimal parameters of the LSSVM are optimized by the CISSA. The simulation test results demonstrate that the VMD–CISSA–LSSVM model has the highest prediction accuracy and stabler prediction results.
Synthesis and Antifouling Activity Evaluation of Analogs of Bromosphaerol, a Brominated Diterpene Isolated from the Red Alga Sphaerococcus coronopifolius
Authors: Prousis Kyriakos C. , Kikionis Stefanos , Ioannou Efstathia , Morgana Silvia , Faimali Marco , Piazza Veronica , Calogeropoulou Theodora , Roussis Vassilios
Published: December 2021

Marine biofouling is an epibiotic biological process that affects almost any kind of submerged surface, causing globally significant economic problems mainly for the shipping industry and aquaculture companies, and its prevention so far has been associated with adverse environmental effects for non-target organisms. Previously, we have identified bromosphaerol (1), a brominated diterpene isolated from the red alga Sphaerococcus coronopifolius, as a promising agent with significant antifouling activity, exerting strong anti-settlement activity against larvae of Amphibalanus (Balanus) amphitrite and very low toxicity. The significant antifouling activity and low toxicity of bromosphaerol (1) motivated us to explore its chemistry, aiming to optimize its antifouling potential through the preparation of a number of analogs. Following different synthetic routes, we successfully synthesized 15 structural analogs (2–16) of bromosphaerol (1), decorated with different functional groups. The anti-settlement activity (EC50) and the degree of toxicity (LC50) of the bromosphaerol derivatives were evaluated using cyprids and nauplii of the cirriped crustacean A. amphitrite as a model organism. Derivatives 2, 4, and 6–16 showed diverse levels of antifouling activity. Among them, compounds 9 and 13 can be considered as well-performing antifoulants, exerting their activity through a non-toxic mechanism.
Potential Anti-Obesity, Anti-Steatosis, and Anti-Inflammatory Properties of Extracts from the Microalgae Chlorella vulgaris and Chlorococcum amblystomatis under Different Growth Conditions
Authors: Regueiras Ana , Huguet Álvaro , Conde Tiago , Couto Daniela , Domingues Pedro , Domingues Maria Rosário , Costa Ana Margarida , Silva Joana Laranjeira da , Vasconcelos Vitor , Urbatzka Ralph
Published: December 2021

Microalgae are known as a producer of proteins and lipids, but also of valuable compounds for human health benefits (e.g., polyunsaturated fatty acids (PUFAs); minerals, vitamins, or other compounds). The overall objective of this research was to prospect novel products, such as nutraceuticals from microalgae, for application in human health, particularly for metabolic diseases. Chlorella vulgaris and Chlorococcum amblystomatis were grown autotrophically, and C. vulgaris was additionally grown heterotrophically. Microalgae biomass was extracted using organic solvents (dichloromethane, ethanol, ethanol with ultrasound-assisted extraction). Those extracts were evaluated for their bioactivities, toxicity, and metabolite profile. Some of the extracts reduced the neutral lipid content using the zebrafish larvae fat metabolism assay, reduced lipid accumulation in fatty-acid-overloaded HepG2 liver cells, or decreased the LPS-induced inflammation reaction in RAW264.7 macrophages. Toxicity was not observed in the MTT assay in vitro or by the appearance of lethality or malformations in zebrafish larvae in vivo. Differences in metabolite profiles of microalgae extracts obtained by UPLC-LC-MS/MS and GNPS analyses revealed unique compounds in the active extracts, whose majority did not have a match in mass spectrometry databases and could be potentially novel compounds. In conclusion, microalgae extracts demonstrated anti-obesity, anti-steatosis, and anti-inflammatory activities and could be valuable resources for developing future nutraceuticals. In particular, the ultrasound-assisted ethanolic extract of the heterotrophic C. vulgaris significantly enhanced the anti-obesity activity and demonstrated that the alteration of culture conditions is a valuable approach to increase the production of high-value compounds.
Bioactive Properties of Peptides and Polysaccharides Derived from Peanut Worms: A Review
Authors: Qi Yi , Zhou Jingyi , Shen Xiaoqin , Chalamaiah Meram , Lv Simin , Luo Hui , Chen Liang
Published: December 2021

Peanut worms (Sipunculids) are unsegmented marine worms that usually inhabit shallow waters. Peanut worms are good source of bioactive compounds including peptides and polysaccharides. Many recent studies have investigated the bioactive properties of peptides and polysaccharides derived from peanut worms in order to enhance their applications in food and pharmaceutical industries. The peptides and polysaccharides isolated from peanut worms have been reported to possess anti-hypertensive, anti-oxidant, immunomodulatory, anti-inflammatory, anti-cancer, anti-hypoxia and wound healing activities through the modulation of various molecular mechanisms. Most researchers used in vitro, cell culture and animal models for the determination of bioactivities of peanut worm derived compounds. However, studies in humans have not been performed considerably. Therefore, it is important to conduct more human studies for better utilization of marine bioactive compounds (peptides and polysaccharides) derived from peanut worms. This review mainly focuses on the bioactive properties of peptides and polysaccharides of peanut worms and their molecular mechanisms.
Optimized Degradation and Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro
Authors: Long Xiaoshan , Hu Xiao , Zhou Shaobo , Xiang Huan , Chen Shengjun , Li Laihao , Liu Shucheng , Yang Xianqing
Published: December 2021

Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded. After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box–Behnken Design (BBD), a new product of GLP-HV will be generated. While using GLP as control, two products of GLP-H (H2O2-treated) and GLP-V (Vc-treated) were also produced. These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed. The results showed that the optimal conditions for H2O2-Vc degradation were as follows: H2O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56 °C. The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose. The SEM analysis demonstrated that H2O2-Vc made the structure loose and broken. Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively. GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive. It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.
Actinomycin X2, an Antimicrobial Depsipeptide from Marine-Derived Streptomyces cyaneofuscatus Applied as a Good Natural Dye for Silk Fabric
Authors: Chen Wei , Ye Kaixiong , Zhu Xiaoji , Zhang Huihui , Si Ranran , Chen Jianing , Chen Zijun , Song Kaili , Yu Zhicheng , Han Bingnan
Published: December 2021

Actinomycins as clinical medicine have been extensively studied, while few investigations were conducted to discover the feasibility of actinomycins as antimicrobial natural dye contributing to the medical value of the functional fabrics. This study was focused on the application of actinomycin X2 (Ac.X2), a peptide pigment cultured from marine-derived Streptomyces cyaneofuscatus, in the dyeing and finishing of silk fabric. The dyeing potential of Ac.X2 with silk vs. cotton fabrics was assessed. As a result, the silk fabric exhibited greater uptake and color fastness with Ac.X2. Through Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) analyses, some changes of chemical property for the dyed fabric and Ac.X2 were studied. The silk fabric dyed with Ac.X2 exhibited good UV protection ability. The antibacterial properties of dyed and finished silk were also evaluated, which exhibited over 90% antibacterial activity even after 20 washing cycles. In addition, the brine shrimp assay was conducted to evaluate the general toxicity of the tested fabric, and the results indicated that the dyed silk fabrics had a good biological safety property.
Investigation of Deep-Sea Ecosystems Using Marker Fatty Acids: Sources of Essential Polyunsaturated Fatty Acids in Abyssal Megafauna
Authors: Svetashev Vasily I.
Published: December 2021

Abyssal seafloor ecosystems cover more than 50% of the Earth’s surface. Being formed by mainly heterotrophic organisms, they depend on the flux of particulate organic matter (POM) photosynthetically produced in the surface layer of the ocean. As dead phytoplankton sinks from the euphotic to the abyssal zone, the trophic value of POM and the concentration of essential polyunsaturated fatty acids (PUFA) decrease. This results in pronounced food periodicity and limitations for bottom dwellers. Deep-sea invertebrate seston eaters and surface deposit feeders consume the sinking POM. Other invertebrates utilize different food items that have undergone a trophic upgrade, with PUFA synthesized from saturated and monounsaturated FA. Foraminifera and nematodes can synthesize arachidonic acid (AA), eicosapentaenoic acid (EPA), while some barophylic bacteria produce EPA and/or docosahexaenoic acid. FA analysis of deep-sea invertebrates has shown high levels of PUFA including, in particular, arachidonic acid, bacterial FA, and a vast number of new and uncommon fatty acids such as 21:4(n-7), 22:4(n-8), 23:4(n-9), and 22:5(n-5) characteristic of foraminifera. We suppose that bacteria growing on detritus having a low trophic value provide the first trophic upgrading of organic matter for foraminifera and nematodes. In turn, these metazoans perform the second-stage upgrading for megafauna invertebrates. Deep-sea megafauna, including major members of Echinodermata, Mollusca, and Polychaeta display FA markers characteristic of bacteria, foraminifera, and nematodes and reveal new markers in the food chain.
Marine Neurotoxins’ Effects on Environmental and Human Health: An OMICS Overview
Authors: Guillotin Sophie , Delcourt Nicolas
Published: December 2021

Harmful algal blooms (HAB), and the consequent release of toxic metabolites, can be responsible for seafood poisoning outbreaks. Marine wildlife can accumulate these toxins throughout the food chain, which presents a threat to consumers’ health. Some of these toxins, such as saxitoxin (STX), domoic acid (DA), ciguatoxin (CTX), brevetoxin (BTX), tetrodotoxin (TTX), and β-N-methylamino-L-alanine (BMAA), cause severe neurological symptoms in humans. Considerable information is missing, however, notably the consequences of toxin exposures on changes in gene expression, protein profile, and metabolic pathways. This information could lead to understanding the consequence of marine neurotoxin exposure in aquatic organisms and humans. Nevertheless, recent contributions to the knowledge of neurotoxins arise from OMICS-based research, such as genomics, transcriptomics, proteomics, and metabolomics. This review presents a comprehensive overview of the most recent research and of the available solutions to explore OMICS datasets in order to identify new features in terms of ecotoxicology, food safety, and human health. In addition, future perspectives in OMICS studies are discussed.
Fracture of the Bone Inducing Its Necrosis as the End Point in the Evolution of Untreated Neuroarthropathy
Authors: Dardari Dured , Penfornis Alfred
Published: December 2021

We describe here the case of a female patient with type I diabetes who developed active Charcot neuroarthropathy in the foot. Due to therapeutic noncompliance, talus necrosis was discovered 2 years after the presentation of neuroarthropathy. The impact of untreated neuroarthropathy on the bone is commonly described as fracture and joint dislocation, but we describe the complete disappearance of the bony structure and its necrosis associated with active neuroarthropathy in a patient who refused offloading.
Kinetic and Metallography Study of the Oxidation at 1250 °C of {Co+Ni}-Based Superalloys Containing Ti to Form MC Carbides
Authors: Berthod Patrice , Ozouaki Wora Synthia Annick , Aranda Lionel , Medjahdi Ghouti , Etienne Erwan
Published: December 2021

Six conventionally cast chromium-rich titanium-containing alloys based on cobalt and nickel with various Co/Ni ratios were considered. They were tested in oxidation in air at 1250 °C for 70 h in a thermo-balance. The mass gain curves were exploited to specify different types of kinetic constants as well as several parameters characterizing the oxide spallation occurring during cooling. The obtained results show that, the higher the Ni content, the slower the mass gain and the better the quality of the protective external chromia scale. Secondly, no dependence of the oxide spallation characteristics on the Co content was clearly noted. Globally, the isothermal oxidation behavior becomes better when Ni is more and more present at the expense of Co. Titanium seems to be playing a particular role in the process of oxidation. It notably leads to the presence of an external thin TiO2 continuous scale beyond the chromia scale. The thermogravimetry records were numerically treated to determine the parabolic constant and the chromia volatilization constant. The values of these constants evidenced a double tendency: chromia growth acceleration and chromia volatilization slow-down. These trends are to be confirmed and further investigated.
Effect of Vanadium and Strain Rate on Hot Ductility of Low-Carbon Microalloyed Steels
Authors: Song Siying , Tian Junyu , Xiao Juan , Fan Lei , Yang Yuebiao , Yuan Qinpan , Gan Xiaolong , Xu Guang
Published: December 2021

Hot tensile tests were conducted in this study to investigate the effect of strain rate (10−3 and 10 s−1) and vanadium content (0.029 and 0.047 wt.%) on the hot ductility of low-carbon microalloyed steels. The results indicate that a hot ductility trough appears at a low strain rate (10−3 s−1) because of the sufficient time for ferrite transformation and the growth of second particles, but it disappears at a high strain rate (10 s−1). The hot ductility is improved with the increase in strain rate at 700 °C or higher temperatures. In addition, with the increase in vanadium content, the large amounts of precipitate and increased ferrite transformation result in poor hot ductility of steels fractured at a low temperature range (600~900 °C). However, when the steel is fractured at a high temperature range (1000~1200 °C), more vanadium in the solid solution in the austenite inhibits the growth of parental austenite grains and results in grain refinement strengthening, slightly improving the hot ductility.
Recovering Value from End-of-Life Batteries by Integrating Froth Flotation and Pyrometallurgical Copper-Slag Cleaning
Authors: Rinne Tommi , Klemettinen Anna , Klemettinen Lassi , Ruismäki Ronja , O’Brien Hugh , Jokilaakso Ari , Serna-Guerrero Rodrigo
Published: December 2021

In this study, industrial lithium-ion battery (LIB) waste was treated by a froth flotation process, which allowed selective separation of electrode particles from metallic-rich fractions containing Cu and Al. In the flotation experiments, recovery rates of ~80 and 98.8% for the cathode active elements (Co, Ni, Mn) and graphite were achieved, respectively. The recovered metals from the flotation fraction were subsequently used in high-temperature Cu-slag reduction. In this manner, the possibility of using metallothermic reduction for Cu-slag reduction using Al-wires from LIB waste as the main reductant was studied. The behavior of valuable (Cu, Ni, Co, Li) and hazardous metals (Zn, As, Sb, Pb), as a function of time as well as the influence of Cu-slag-to-spent battery (SB) ratio, were investigated. The results showcase a suitable process to recover copper from spent batteries and industrial Cu-slag. Cu-concentration decreased to approximately 0.3 wt.% after 60 min reduction time in all samples where Cu/Al-rich LIB waste fraction was added. It was also showed that aluminothermic reduction is effective for removing hazardous metals from the slag. The proposed process is also capable of recovering Cu, Co, and Ni from both Cu-slag and LIB waste, resulting in a secondary Cu slag that can be used in various applications.
Lipid Alterations in African American Men with Prostate Cancer
Authors: Ravindran Anindita , Piyarathna Danthasinghe Waduge Badrajee , Gohlke Jie , Putluri Vasanta , Soni Tanu , Lloyd Stacy , Castro Patricia , Pennathur Subramaniam , Jones Jeffrey A. , Ittmann Michael , Putluri Nagireddy , Michailidis George , Rajendiran Thekkelnaycke M. , Sreekumar Arun
Published: December 2021

African-American (AA) men are more than twice as likely to die of prostate cancer (PCa) than European American (EA) men. Previous in silico analysis revealed enrichment of altered lipid metabolic pathways in pan-cancer AA tumors. Here, we performed global unbiased lipidomics profiling on 48 matched localized PCa and benign adjacent tissues (30 AA, 24 ancestry-verified, and 18 EA, 8 ancestry verified) and quantified 429 lipids belonging to 14 lipid classes. Significant alterations in long chain polyunsaturated lipids were observed between PCa and benign adjacent tissues, low and high Gleason tumors, as well as associated with early biochemical recurrence, both in the entire cohort, and within AA patients. Alterations in cholesteryl esters, and phosphatidyl inositol classes of lipids delineated AA and EA PCa, while the levels of lipids belonging to triglycerides, phosphatidyl glycerol, phosphatidyl choline, phosphatidic acid, and cholesteryl esters distinguished AA and EA PCa patients with biochemical recurrence. These first-in-field results implicate lipid alterations as biological factors for prostate cancer disparities.
Analysis of Preload of Three-Stator Ultrasonic Motor
Authors: Li Zheng , Zhao Hui , Che Shuai , Chen Xuetong , Sun Hexu
Published: December 2021

The pre-pressure device of the ultrasonic motor plays a vital role in the design of the entire motor structure, the contact state of the stator and rotor of the motor, dynamic properties of the stator, friction and wear characteristics of the rotor; even the mechanical behaviors of the entire electric machinery have a profound impact. Appropriate pre-pressure is conducive to the smooth operation of the ultrasonic motor, so that the output performance remains excellent, reducing wear and effectively extend the service life of the motor. Therefore, the research on pre-stress is of great significance, as it can better optimize the structure of the three-stator ultrasonic motor and lay the foundation for the stable operation of the motor. First, this paper introduces the construction of the motor as a whole and the pre-pressure device briefly described the working mechanism of the motor, and then introduces the influence of the pre-pressure on the stator and rotor contact models, the position of the constant velocity point, and the modal frequency. Finally, the motor output under different pre-pressures is discussed. The performance experiment has determined the optimal pre-pressure interval, which provides help for its subsequent optimization.
Controlling Microdroplet Inner Rotation by Parallel Carrier Flow of Sesame and Silicone Oils
Authors: Yoshimura Hibiki , Tanaka Daiki , Furuya Masahiro , Sekiguchi Tetsushi , Shoji Shuichi
Published: December 2021

We developed a method for passively controlling microdroplet rotation, including interior rotation, using a parallel flow comprising silicone and sesame oils. This device has a simple 2D structure with a straight channel and T-junctions fabricated from polydimethylsiloxane. A microdroplet that forms upstream moves into the sesame oil. Then, the largest flow velocity at the interface of the two oil layers applies a rotational force to the microdroplet. A microdroplet in the lower oil rotates clockwise while that in the upper oil rotates anti-clockwise. The rotational direction was controlled by a simple combination of sesame and silicone oils. Droplet interior flow was visualized by tracking microbeads inside the microdroplets. This study will contribute to the efficient creation of chiral molecules for pharmaceutical and materials development by controlling rotational direction and speed.
Passivated Porous Silicon Membranes and Their Application to Optical Biosensing
Authors: Whyte Ferreira Clara , Vercauteren Roselien , Francis Laurent
Published: December 2021

A robust fabrication method for stable mesoporous silicon membranes using standard microfabrication techniques is presented. The porous silicon membranes were passivated through the atomic layer deposition of different metal oxides, namely aluminium oxide Al2O3, hafnium oxide HfO2 and titanium oxide TiO2. The fabricated membranes were characterized in terms of morphology, optical properties and chemical properties. Stability tests and optical probing noise level determination were also performed. Preliminary results using an Al2O3 passivated membranes for a biosensing application are also presented for selective optical detection of Bacillus cereus bacterial lysate. The biosensor was able to detect the bacterial lysate, with an initial bacteria concentration of 106 colony forming units per mL (CFU/mL), in less than 10 min.
Mineralogical Characterization and Acid Pretreatment of a Gold Calcine Leach Residue
Authors: Bare Godfrey Tinashe , Mbayo Jean Jacques Kalombo , Ndlovu Sehliselo , Shemi Alan , Chipise Liberty
Published: December 2021

Miners around Zimbabwe used to supply gold concentrates from sulphide flotation to the Kwekwe Roasting Plant (Zimbabwe) for toll treatment. The concentrates were roasted in Edward’s roasters and the calcine product was leached by cyanidation. Due to inefficient roasting, overall gold recoveries of 75–80% left behind a rich calcine leach residue at the Kwekwe Roasting Plant. The characterization performed to establish a potential process route involved several techniques, such as X-ray diffraction (XRD), scanning electron microscopy (SEM), fire assaying and inductively coupled plasma (ICP). Assays conducted on samples from the 350,000 tons tailings dam residue, created over the operational years, gave an average Au grade of 8.58 g/t and 12.54 g/t for Ag. The base metals assayed—0.11% Cu, 0.10% Pb, 0.17% Zn and 26.05% Fe. SiO2 (36.1%), Fe2O3 (36.9%), Mg3Si4O10 (OH)2 (8.9%), NaAlSi3O8 (6.9%), and Fe3O4 (6.4%)—were the major mineral phases in the cyanide leach residue. SEM gold scans on 24 polished sections showed only 2 discrete gold particles of less than 5 µm, with one partially liberated and associated with quartz, while the other was fully liberated. Therefore, the particulate gold in the calcine leach residue was negligible. It was deduced from the analysis after ultrafine milling (P80 < 5 µm) followed by cyanidation that 68.53% of the gold was sub-microscopic. Direct cyanidation using bottle roll resulted in only 2.33% of the total gold being leachable, indicating that the calcine leach residue was highly refractory. Diagnostic leaching by sequential use of acids in order of their strength resulted in HCl leachable phases (CaCO3, CaMg(CO3)2, PbS, Fe1-XS, and Fe2O3) freeing 4.2% of the total Au during subsequent cyanidation leach. H2SO4 leachable phases (Cu–Zn sulphides, labile FeS2) released an additional 26.57% during cyanidation, whereas HNO3 leachable phases (FeS2, FeAsS) released a further 20.98% of Au. After acid treatment and subsequent cyanidation, hot caustic leach of the residue followed by carbon in pulp resulted 4.43% of the total gold being eluted. Therefore, 4.43% of the total gold was surface bound. From the analysis after diagnostic acid leaching, it was deduced that a total of 54.08% of the gold was in the acid-leachable phase. Due to cost and environmental considerations, H2SO4 was selected for the evaluation of acid digestion as a pretreatment stage followed by cyanidation. Increasing the H2SO4 strength for the pretreatment of the calcine leach residue increased gold recoveries during cyanidation.
On the Quantum Chemical Nature of Lead(II) “Lone Pair”
Authors: Gourlaouen Christophe , Piquemal Jean-Philip
Published: December 2021

We study the quantum chemical nature of the Lead(II) valence basins, sometimes called the lead “lone pair”. Using various chemical interpretation tools, such as molecular orbital analysis, natural bond orbitals (NBO), natural population analysis (NPA) and electron localization function (ELF) topological analysis, we study a variety of Lead(II) complexes. A careful analysis of the results shows that the optimal structures of the lead complexes are only governed by the 6s and 6p subshells, whereas no involvement of the 5d orbitals is found. Similarly, we do not find any significant contribution of the 6d. Therefore, the Pb(II) complexation with its ligand can be explained through the interaction of the 6s2 electrons and the accepting 6p orbitals. We detail the potential structural and dynamical consequences of such electronic structure organization of the Pb (II) valence domain.
Comparison of Experimental Strategies to Study l-Type Amino Acid Transporter 1 (LAT1) Utilization by Ligands
Authors: Huttunen Johanna , Agami Mahmoud , Tampio Janne , Montaser Ahmed B. , Huttunen Kristiina M.
Published: December 2021

l-Type amino acid transporter 1 (LAT1), expressed abundantly in the brain and placenta and overexpressed in several cancer cell types, has gained a lot of interest in drug research and development, as it can be utilized for brain-targeted drug delivery, as well as inhibiting the essential amino acid supply to cancer cells. The structure of LAT1 is today very well-known and the interactions of ligands at the binding site of LAT1 can be modeled and explained. However, less is known of LAT1′s life cycle within the cells. Moreover, the functionality of LAT1 can be measured by several different methods, which may vary between the laboratories and make the comparison of the results challenging. In the present study, the usefulness of indirect cis-inhibition methods and direct cellular uptake methods and their variations to interpret the interactions of LAT1-ligands were evaluated. Moreover, this study also highlights the importance of understanding the intracellular kinetics of LAT1-ligands, and how they can affect the regular function of LAT1 in critical tissues, such as the brain. Hence, it is discussed herein how the selected methodology influences the outcome and created knowledge of LAT1-utilizing compounds.
IR-Spectroscopic Study of Complex Formation of Nitrogen Oxides (NO, N2O) with Cationic Forms of Zeolites and the Reactivity of Adsorbed Species in CO and CH4 Oxidation
Authors: Kustov Alexander L. , Kustov Leonid M.
Published: December 2021

The formation of complexes and disproportionation of nitrogen oxides (NO, N2O) on cationic forms of LTA, FAU, and MOR zeolites was investigated by diffuse-reflectance IR spectroscopy. N2O is adsorbed on the samples under study in the molecular form and the frequencies of the first overtone of the stretching vibrations ν10–2 and the combination bands of the stretching vibrations with other vibrational modes for N2O complexes with cationic sites in zeolites (ν30–1 + ν10–1, ν10–1 + δ0–2) are more significantly influenced by the nature of the zeolite. The presence of several IR bands in the region of 2400–2600 cm−1 (the ν10–1 + δ0–2 transitions) for different zeolite types was explained by the availability of different localization sites for cations in these zeolites. The frequencies in this region also depend on the nature of the cation (its charge and radius). The data can be explained by the specific geometry of the N2O complex formed, presumably two-point adsorption of N2O on a cation and a neighboring oxygen atom of the framework. Adsorption of CO or CH4 on the samples with preliminarily adsorbed N2O at 20–180 °C does not result in any oxidation of these molecules. NO+ and N2O3 species formed by disproportionation of NO are capable of oxidizing CO and CH4 molecules to CO2, whereas NOx is reduced simultaneously to N2 or N2O. The peculiarities in the behavior of cationic forms of different zeolites with respect to adsorbed nitrogen oxides determined by different density and localization of cations have been established.