bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Bayesian Deep Learning for Electric Vehicle Charging Station Load Forecasting
Authors: Zhou Dan , Guo Zhonghao , Xie Yuzhe , Hu Yuheng , Jiang Da , Feng Yibin , Liu Dong
Published: August 2022

In recent years, replacing internal combustion engine vehicles with electric vehicles has been a significant option for supporting reducing carbon emissions because of fossil fuel shortage and environmental contamination. However, the rapid growth of electric vehicles (EVs) can bring new and uncertain load conditions to the electric network. Precise load forecasting for EV charging stations becomes vital to reduce the negative influence on the grid. To this end, a novel day-ahead load forecasting method is proposed to forecast loads of EV charging stations with Bayesian deep learning techniques. The proposed methodological framework applies long short-term memory (LSTM) network combined with Bayesian probability theory to capture uncertainty in forecasting. Based on the actual operational data of the EV charging station collected on the Caltech campus, the experiment results show the superior performance of the proposed method compared with other methods, indicating significant potential for practical applications.
Batteries and Hydrogen Storage: Technical Analysis and Commercial Revision to Select the Best Option
Authors: Andújar José Manuel , Segura Francisca , Rey Jesús , Vivas Francisco José
Published: August 2022

This paper aims to analyse two energy storage methods—batteries and hydrogen storage technologies—that in some cases are treated as complementary technologies, but in other ones they are considered opposed technologies. A detailed technical description of each technology will allow to understand the evolution of batteries and hydrogen storage technologies: batteries looking for higher energy capacity and lower maintenance, while hydrogen storage technologies pursuing better volumetric and gravimetric densities. Additionally, as energy storage systems, a mathematical model is required to know the state of charge of the system. For this purpose, a mathematical model is proposed for conventional batteries, for compressed hydrogen tanks, for liquid hydrogen storage and for metal hydride tanks, which makes it possible to integrate energy storage systems into management strategies that aim to solve the energy balance in plants based on hybrid energy storage systems. From the technical point of view, most batteries are easier to operate and do not require special operating conditions, while hydrogen storage methods are currently functioning at the two extremes (high temperatures for metal and complex hydrides and low temperatures for liquid hydrogen or physisorption). Additionally, the technical comparison made in this paper also includes research trends and future possibilities in an attempt to help plan future policies.
The Conundrums of Illicit Crude Oil Refineries in Nigeria and Its Debilitating Effects on Nigeria’s Economy: A Legal Approach
Authors: Olujobi Olusola Joshua , Olarinde Elizabeta Smaranda , Yebisi Tunde Ebenezer
Published: August 2022

Nigeria’s oil industry encounters crude oil theft in commercial quantities, which is often exported to neighbouring countries. This has occasioned a loss of revenue and has caused environmental pollution due to oil spillages. There is a need for a stringent legal framework to combat the menace caused by incessant crude oil thefts, pipeline vandalisation by militants, and inadequate maintenance of existing crude oil refineries. The study adopts doctrinal legal research methods and a conceptual approach with the consideration of primary and secondary sources of law, for instance, the Petroleum Industry Act 2021, the Nigeria Extractive Industry Transparency Initiative (NEITI Act 2007, International Conventions, law textbooks and peer-reviewed journals. The justification for using the method was to establish the trustworthiness of the findings on illicit crude oil refineries. The findings reveal that the Nigerian government has lost more than 150,000 barrels of crude oil daily valued at USD six billion as a result of crude oil theft. This has reduced oil revenues, which ought to have added to the national treasury. The Petroleum Production and Distribution (Anti-Sabotage Act) 2007, which proscribes disruption of petroleum products in Nigeria, has not been diligently enforced. There is also an absence of a specific oil and gas legal framework criminalising crude oil theft. Section 3(e)(f)(iv) of the Nigeria Security and Civil Defence Corps Act only offers pipeline security as one of the functions of the corps, without distinctly stating the penalties to be imposed on those damaging crude oil pipelines. The study designs a hybrid model for the renovation of the country’s crude oil refineries. It also advocates the need to redefine legal regimes on illegal oil refineries by amending the Petroleum Industry Act to include specifically illegal oil refineries provision and to effectively criminalise crude oil theft. The implications of the main results are as follows: criminalising crude oil theft and pipeline vandalisation with vigorous punishments will serve as deterrence to others in the sector, increase revenues for the government and reduce environmental pollution.
Exploring the Spatial and Temporal Distribution of Frigate Tuna (Auxis thazard) Habitat in the South China Sea in Spring and Summer during 2015–2019 Using Fishery and Remote Sensing Data
Authors: Zhou Xingxing , Chen Zuozhi , Xiong Pengli , Cai Yancong , Li Jie , Zhang Peng , Zhang Jun , Li Miao , Fan Jiangtao
Published: August 2022

Frigate tuna Auxis thazard fishery is considered a potential marine resource in the open South China Sea (SCS). However, little is known about the spatial and temporal distribution of its habitat, and how this may respond to environmental changes. Using fish survey and remote sensing data from 2015–2019, we applied generalized additive models to identify relationships between environmental factors and the distribution of A. thazard in the SCS. To examine seasonal patterns in the habitat of A. thazard in the SCS, we generated a habitat suitability index model using environmental factors screened by generalized additive models. Results showed that A. thazard migrates from south to north in the SCS, and its suitable habitat is patchily distributed. Significant environmental factors affecting the habitat of A. thazard distribution were different in different seasons; we demonstrated A. thazard to be sensitive to Chl-a in spring (optimum 0.155, optimal range ~0.1252–0.1840), and in summer to be sensitive to SST (optimum 30.405, optimal range ~29.789–31.021) and SSH (optimum 0.741, optimal range ~0.618–0.864). Suitable habitat in spring occurs mainly in northeastern areas, while in summer it occurs mainly around the southeastern Nansha Islands. Compared with that in spring, the area of suitable habitat increases in summer, as does the habitat suitability index of the entire sea area. These results improve our understanding of environmental factors that affect the distribution of A. thazard habitat in the SCS, and provide a scientific basis for the development and management of A. thazard resources.
Influence of Preferments on the Physicochemical and Sensory Quality of Traditional Panettone
Authors: Jamanca-Gonzales Nicodemo C. , Ocrospoma-Dueñas Robert W. , Quintana-Salazar Norma B. , Siche Raúl , Silva-Paz Reynaldo J.
Published: August 2022

In Peru, panettones are consumed in July and December. The main ingredient of panettones is wheat flour, which can be replaced with substitute flours to improve their nutritional, textural and sensory properties. This study aimed to evaluate the physicochemical, textural and sensory characteristics of panettones produced with three preferments, namely, biga (PB), sourdough (PMM) and sponge (PE), with the substitution of red quinoa flour and amaranth compared with a commercial product (PC). A completely randomized design with four experimental treatments was used to evaluate the total carbohydrate content, ash, total energy, fat, moisture, protein, color and texture profile. In addition, sensory characteristics were evaluated by 80 consumers using the CATA method; the purchase intention and preference ranking were also investigated. The results showed better sensory characteristics of panettones produced with preferments compared with a commercial product with similar characteristics. The sponge preferment presented better sensory characteristics with a profile of sweet, spongy, vanilla odor and moist texture, along with greater acceptability, preference and purchase intention, followed closely by the biga. It was concluded that the sponge preferment presented better sensory properties, which were correlated with its texture profile as manifested by an intermediate hardness, good elasticity and cohesiveness, which translated into greater acceptability, preference and purchase intention.
Effect of Dietary Enrichment with Flaxseed, Vitamin E and Selenium, and of Market Class on the Broiler Breast Meat—Part 2: Technological and Sensorial Traits
Authors: Albergamo Ambrogina , Vadalà Rossella , Metro Daniela , Giuffrida Daniele , Monaco Francesco , Pergolizzi Stefano , Leonardi Michelangelo , Bartolomeo Giovanni , Petracci Massimiliano , Cicero Nicola
Published: August 2022

The influence of diet enrichment with flaxseed, selenium and vitamin E, and market class on breast meat was investigated in terms of technological and sensorial quality of breast meat. A randomized complete block design with an experimental unit of n = 6000 broilers receiving a standard or enriched diet, and slaughtered at 37 (light class), 47 (medium class), or 57 (heavy class) days of life, was developed. Then, enriched and standard breast muscles from every market class were studied for their technological and sensorial traits—both at 24 h post-mortem and after one month of frozen storage—by a statistical multiple linear model. Redness and yellowness of muscles significantly (p < 0.05) increased and decreased with increasing market age. Moreover, the yellowness significantly (p < 0.05) raised after frozen storage. However, obtained data were always indicative of a normal meat color. The water holding capacity improved following fed enrichment and significantly (p < 0.05) worsened after frozen storage. For the sensory analysis, juiciness and chewing rest of meat resulted significantly (p < 0.05) improved with increasing slaughtering age and diet enrichment, as well as their mutual interaction, while they deteriorated after frozen storage. Overall, fresh and enriched muscles from heavy broilers had the best technological and sensorial traits, thus, confirming that market size and diet should be highly considered to obtain breast meat with greater consumer acceptance.
Effects of Fruit Storage Temperature and Time on Cloud Stability of Not from Concentrated Apple Juice
Authors: Wang Haifen , Yuan Junwei , Chen Lan , Ban Zhaojun , Zheng Yanli , Jiang Yuqian , Jiang Yunbin , Li Xihong
Published: August 2022

Apple juice that is designated ‘Not from concentrated’ (NFC) is now increasingly popular with consumers due to its unique taste and rich nutritional value. However, layered precipitation and instability have emerged as serious technical problems that restrict the viability of the NFC apple juice industry. This study researched the influence of water-cored ‘Fuji’ apple fruit storage under different temperatures (0, 20 °C) and times (0, 9, 18, 30, 60 days) on the turbidity stability of NFC apple juice. Changes in the physicochemical properties (juice yield, pH, total soluble solids and titratable acid), turbidity stability (turbidity and particle size) and precipitation sensitive substances (insoluble starch, total phenolics, soluble protein and pectin) of NFC apple juice were determined, combined with the respiratory rates and ethylene release of apples, in order to study post-harvest regulation and control of processed fruit. Results indicated that fruit storage temperature and time significantly guided the turbidity stability of NFC apple juice. As a typical respiratory climacteric fruit, apple fruit stored 45 days at 0 °C and 15 days at 20 °C gained the best juice stability, respectively. This is basically consistent with the respiratory peak of fruit when processing raw materials. During the post-ripening process, the insoluble starch in apple gradually hydrolyzed into fructose and glucose, while total phenolics diminished and water-soluble pectin content increased. On the other hand, the amounts of pectin, soluble protein and phenolics in fruit juice declined as the fruit aged in the late storage period (stored 75 days at 0 °C and 40 days at 20 °C). Meanwhile particle size became larger and the turbidity stability of cloudy juices also decreased. This study’s results will provide a sound theoretical basis for improving the turbidity stability of NFC apple juice by regulating the physiological state of processed raw materials.
Pomegranate (Punica granatum L.) Attenuates Neuroinflammation Involved in Neurodegenerative Diseases
Authors: Mehdi Alami , Lamiae Benchagra , Samira Boulbaroud , Ramchoun Mhamed , Abdelouahed Khalil , Tamas Fulop , Hicham Berrougui
Published: August 2022

Food scientists have studied the many health benefits of polyphenols against pernicious human diseases. Evidence from scientific studies has shown that earlier healthy lifestyle changes, particularly in nutrition patterns, can reduce the burden of age-related diseases. In this context, a large number of plant-derived components belonging to the class of polyphenols have been reported to possess neuroprotective benefits. In this review, we examined studies on the effect of dietary polyphenols, notably from Punica granatum L., on neurodegenerative disease, including Alzheimer’s disease, which is symptomatically characterized by impairment of cognitive functions. Clinical trials are in favor of the role of some polyphenols in maintaining neuronal homeostasis and attenuating clinical presentations of the disease. However, discrepancies in study design often bring inconsistent findings on the same component and display differences in their effectiveness due to interindividual variability, bioavailability in the body after administration, molecular structures, cross-blood-brain barrier, and signaling pathways such as nuclear factor kappa B (NF-κB). Based on preclinical and clinical trials, it appears that pomegranate may prove valuable in treating neurodegenerative disorders, including Alzheimer’s disease (AD) and Parkinson’s disease (PD). Therefore, due to the lack of information on human clinical trials, future in-depth studies, focusing on human beings, of several bioactive components of pomegranate’s polyphenols and their synergic effects should be carried out to evaluate their curative treatment.
Fattening in Saline and Alkaline Water Improves the Color, Nutritional and Taste Quality of Adult Chinese Mitten Crab Eriocheir sinensis
Authors: Wang Shihui , Guo Kun , Luo Liang , Zhang Rui , Xu Wei , Song Yingying , Zhao Zhigang
Published: August 2022

The majority of pond-reared Chinese mitten crab (Eriocheir sinensis) grow and fatten in freshwater. Previous studies illustrated that E. sinensis cultured in saline-alkaline water in outdoor environments showed a higher quality than that cultured in freshwater. However, it is still unclear whether salinity or alkalinity has an important positive effect on the quality of E. sinensis. This study aimed to investigate the gonadal development, edible yield, coloration, and nutritional and flavor quality of E. sinensis fattening in saline and alkaline water indoors. Results showed that there were no significant changes observed in gonadosomatic index (GSI) and other edible parameters among freshwater (FW), saline water (SW), and alkaline water (AW) during the 55-day fattening period (p > 0.05). Significantly higher a* and b* values of freeze-dried female carapace were observed fattening in SW and AW compared with that of FW (p < 0.05). The crude protein in gonad and male muscle, moisture in female muscle, and crude lipid in male muscle increased significantly from FW to SW and AW (p < 0.05). Better nutritional and flavor values were also detected in male hepatopancreas and muscles. In conclusion, numerous advantages of fattening in SW and AW were observed, including the improvement of carotenoid accumulation in freeze-dried carapace, DHA, EPA, total essential free amino acids (∑EFAA), total free amino acids (∑FAA), and total umami values (∑TUV) contents in male hepatopancreas and muscle.
Whole-Genome Analysis of Staphylococcus aureus Isolates from Ready-to-Eat Food in Russia
Authors: Mikhaylova Yulia , Shelenkov Andrey , Chernyshkov Aleksey , Tyumentseva Marina , Saenko Stepan , Egorova Anna , Manzeniuk Igor , Akimkin Vasiliy
Published: August 2022

This study provides a thorough investigation of a diverse set of antimicrobial resistant (AMR) Staphylococcus aureus isolates collected from a broad range of ready-to-eat (RTE) food in various geographic regions of Russia ranging from Pskov to Kamchatka. Thirty-five isolates were characterized using the whole genome sequencing (WGS) analysis in terms of clonal structure, the presence of resistance and virulence determinants, as well as plasmid replicon sequences and CRISPR/Cas systems. To the best of our knowledge, this is the first WGS-based surveillance of Russian RTE food-associated S. aureus isolates. The isolates belonged to fifteen different multilocus sequence typing (MLST)-based types with a predominant being the ones of clonal complex (CC) 22. The isolates studied can pose a threat to public health since about 40% of the isolates carried at least one enterotoxin gene, and 70% of methicillin-resistant (MRSA) isolates carried a tsst1 gene encoding a toxin that may cause severe acute disease. In addition, plasmid analysis revealed some important characteristics, e.g., Rep5 and Rep20 plasmid replicons were a “signature” of MRSA CC22. By analyzing the isolates belonging to the same/single strain based on cgMLST analysis, we were able to identify the differences in their accessory genomes marking their dynamics and plasticity. This data is very important since S. aureus isolates studied and RTE food, in general, represent an important route of transmission and dissemination of multiple pathogenic determinants. We believe that the results obtained will facilitate performing epidemiological surveillance and developing protection measures against this important pathogen in community settings.
Quantitative Bio-Mapping of Salmonella and Indicator Organisms at Different Stages in a Commercial Pork Processing Facility
Authors: Bueno López Rossy , Vargas David A. , Jimenez Reagan L. , Casas Diego E. , Miller Markus F. , Brashears Mindy M. , Sanchez-Plata Marcos X.
Published: August 2022

The purpose of this study was to develop a quantitative baseline of indicator organisms and Salmonella by bio-mapping throughout the processing chain from harvest to final product stages within a commercial conventional design pork processing establishment. Swab samples were taken on the harvest floor at different processing steps, gambrel table, after polisher, before final rinse, after the final rinse, post snap chill, and after peroxyacetic acid (PAA) application, while 2-pound product samples were collected for trim and ground samples. The samples were subjected to analysis for indicator microorganism enumeration, Aerobic Count (AC), Enterobacteriaceae (EB), and generic Escherichia coli (EC), with the BioMérieux TEMPO®. Salmonella prevalence and enumeration was evaluated using the BAX® System Real-Time Salmonella and the SalQuant™ methodology. Microbial counts were converted to Log Colony-forming units (CFU) on a per mL, per g or per sample basis, presented as LogCFU/mL, LogCFU/g and LogCFU/sample, prior to statistical analysis. All indicator microorganisms were significantly reduced at the harvest floor (p-value < 0.001), from gambrel table to after PAA cabinet location. The reduction at harvest was 2.27, 2.46 and 2.24 LogCFU/mL for AC, EB and EC, respectively. Trim sample values fluctuated based on cut, with the highest average AC count found at neck trim (2.83 LogCFU/g). Further process samples showed the highest AC count in sausage with a mean of 5.28 LogCFU/g. EB counts in sausage (3.19 LogCFU/g) showed an evident increase, compared to the reduction observed at the end of harvest and throughout trim processing. EC counts showed a similar trend to EB counts with the highest value found in sausage links (1.60 LogCFU/g). Statistical microbial process control (SPC) parameters were also developed for each of the indicator microorganisms, using the overall mean count (X=), the Lower control limit (LCL) and Upper control limit (UCL) at each sampling location. For Salmonella prevalence, a total of 125/650 samples were found positive (19%). From those positive samples, 47 samples (38%) were suitable for enumeration using the BAX® System SalQuant™, the majority detected at the gambrel table location. From those enumerable samples, 60% were estimated to be between 0.97 and 1.97 LogCFU/sample, while the rest (40%) were higher within the 2.00–4.02 LogCFU/sample range. This study provides evidence for the application of indicator and pathogen quantification methodologies for food safety management in commercial pork processing operations.
“It’s All Bread, All the Way Down”: The Baby-Sitters Club Club as Hyperfanfiction
Authors: Scott Suzanne
Published: August 2022

In February 2016, co-hosts Jack Shepherd and Tanner Greenring launched their comedy podcast The Baby-Sitters Club Club. The “joke” at the center of the podcast was, of course, that two adult, cishet, white men were exhaustively recapping and dissecting a book series from the 1980s and 1990s that was predominantly popular with adolescent girls. What began as a podcast designed to poke fun at the co-hosts’ serious “fannish” analysis of a nostalgic series of novels for girls, evolved into an elaborate string of “fan” theories, literary close readings, and (inter)textual expansion. Building on Paul Booth’s discussion of hyperfans, this article theorizes the absurdist worldbuilding, mythology and character development, and intertextual play performed by the hosts of TBSCC as a form of hyperfanfiction.
Associations between Psychological Distress, Perceived Social Support and Physical Activity Level in Spanish Adults with Depression
Authors: Denche-Zamorano Ángel , Pastor-Cisneros Raquel , Carlos-Vivas Jorge , Franco-García Juan Manuel , Pereira-Payo Damián , Barrios-Fernandez Sabina , Rojo-Ramos Jorge , Mendoza-Muñoz María
Published: August 2022

Perceived social support (PSS) and physical activity (PA) could help to reduce psychological distress in people with depression. This study aims to analyse the associations between (a) mental health and its dimensions through the Goldberg General Health Questionnaire (GHQ-12), (b) the PA level (PAL), and c) the PSS in the Spanish adult population with psychological distress. This cross-sectional study is based on Spanish National Health Survey 2017 data, including 1670 adults with depression. A descriptive analysis was performed. Differences in medians between sexes were analysed using the Mann–Whitney U test. The Chi-square test was used to assess the independence between sex and PAL. The Kruskal–Wallis’ test was performed to analyse possible baseline differences between PAL and continuous variables derived from the GHQ-12. Finally, a correlation study was conducted between the generated variables and the GHQ-12 items, together with the PAL and the Duke-UNC-11, using Spearman’s rho correlation coefficients. Weak inverse correlations were found between the GHQ-12 and PAL (rho: −0.214); and PSS (r: −0.286). PAL and PSS showed weak inverse correlations with successful coping (rho: −0.216 and r: −0.265), self-esteem (rho: −0.209 and r: −0.283), and stress (rho: −0.130 and r: −0.232). Thus, higher PAL and SSP is associated with lower psychological distress.
Tackling Thyroid Cancer in Europe—The Challenges and Opportunities
Authors: Horgan Denis , Führer-Sakel Dagmar , Soares Paula , Alvarez Clara V. , Fugazzola Laura , Netea-Maier Romana T. , Jarzab Barbara , Kozaric Marta , Bartes Beate , Schuster-Bruce James , Dal Maso Luigino , Schlumberger Martin , Pacini Furio
Published: August 2022

Thyroid cancer (TC) is the most common malignancy of the endocrine system that affects the thyroid gland. It is usually treatable and, in most cases, curable. The central issues are how to improve knowledge on TC, to accurately identify cases at an early stage that can benefit from effective intervention, optimise therapy, and reduce the risk of overdiagnosis and unnecessary treatment. Questions remain about management, about treating all patients in referral centres, and about which treatment should be proposed to any individual patient and how this can be optimised. The European Alliance for Personalised Medicine (EAPM) hosted an expert panel discussion to elucidate some of the challenges, and to identify possible steps towards effective responses at the EU and member state level, particularly in the context of the opportunities in the European Union’s evolving initiatives—notably its Beating Cancer Plan, its Cancer Mission, and its research funding programmes. Recommendations emerging from the panel focus on improved infrastructure and funding, and on promoting multi-stakeholder collaboration between national and European initiatives to complement, support, and mutually reinforce efforts to improve patient care.
Identifying the High-Risk Population for COVID-19 Transmission in Hong Kong Leveraging Explainable Machine Learning
Authors: Jiang Zhihan , Yip Ka-Man , Zhang Xinchen , Deng Jing , Wong Wilfred , So Hung-Kwan , Ngai Edith C. H.
Published: August 2022

The worldwide spread of COVID-19 has caused significant damage to people’s health and economics. Many works have leveraged machine learning models to facilitate the control and treatment of COVID-19. However, most of them focus on clinical medicine and few on understanding the spatial dynamics of the high-risk population for transmission of COVID-19 in real-world settings. This study aims to investigate the association between population features and COVID-19 transmission risk in Hong Kong, which can help guide the allocation of medical resources and the implementation of preventative measures to control the spread of the pandemic. First, we built machine learning models to predict the number of COVID-19 cases based on the population features of different tertiary planning units (TPUs). Then, we analyzed the distribution of cases and the prediction results to find specific characteristics of TPUs leading to large-scale outbreaks of COVID-19. We further evaluated the importance and influence of various population features on the prediction results using SHAP values to identify indicators for high-risk populations for COVID-19 transmission. The evaluation of COVID-19 cases and the TPU dataset in Hong Kong shows the effectiveness of the proposed methods. The top three most important indicators are identified as people in accommodation and food services, low income, and high population density.
Soil Pore Network Complexity Changes Induced by Wetting and Drying Cycles—A Study Using X-ray Microtomography and 3D Multifractal Analyses
Authors: de Oliveira Jocenei A. T. , Cássaro Fábio A. M. , Posadas Adolfo N. D. , Pires Luiz F.
Published: August 2022

Soils are dynamic and complex systems in their natural state, which are subjected to profound changes due to management. Additionally, agricultural soils are continuously exposed to wetting and drying (W-D) cycles, which can cause modifications in the complexity of their pores. Thus, we explore how successive W-D cycles can affect the pore network of an Oxisol under contrasting managements (conventional tillage—CT, minimum tillage—MT, no tillage—NT, and secondary forest—F). The complexity of the soil pore architecture was evaluated using a 3D multifractal approach combined with lacunarity, Shannon’s entropy, and pore geometric parameters. Our results showed that the multifractal approach effectively identified and quantified the changes produced in the soil pore architecture by the W-D cycles. The lacunarity curves revealed important aspects of the modifications generated by these cycles. Samples under F, NT, and MT suffered the most significant changes. Pore connectivity and tortuosity were largely affected by the cycles in F and NT. Our findings demonstrated that the 3D geometric parameters and normalized Shannon’s entropy are complementary types of analysis. According to the adopted management, they allowed us to separate the soil into two groups according to their similarities (F and NT; CT and MT).
Development of Advanced Competencies in Physiotherapy: Impact of a Single-Blinded Controlled Trial on Ethics Competence
Authors: Cortés-Amador Sara , Arnal-Gómez Anna , Marques-Sule Elena , Hernández-Guillén David , Tolsada-Velasco Catalina , Espí-López Gemma V.
Published: August 2022

Background: Innovation in the training of future physiotherapy professionals through the use of collaborative learning could be an effective method for developing advanced competencies such as professional ethics. This study aimed at comparing the effects of cooperative learning and individual learning on the knowledge of professional ethics, the perception of knowledge regarding professional ethics, the teaching quality assessment and satisfaction in future physiotherapy professionals. Methods: A prospective, assessor-blinded, controlled trial was performed. A 12-week program was carried out with future physiotherapy professionals. The cooperative learning group was based on group activities, while the individual learning group performed the same activities with an individual approach. Knowledge, perception of knowledge regarding professional ethics, teaching quality and satisfaction were assessed. Results: A total of 216 participants completed the study (cooperative group n = 106; individual group n = 110). The cooperative learning group showed higher knowledge and perception of knowledge regarding professional ethics compared to the individual learning group (p < 0.001 and p < 0.001, respectively). Additionally, the cooperative learning group reported higher scores in the teaching materials, attitude towards future professionals and the teacher’s global score. Conclusions: Cooperative learning showed a positive impact on developing advanced competencies such as knowledge and perception of knowledge regarding professional ethics. Both methodologies showed adequate results in the assessment of teaching quality and satisfaction.
Long-Time Trend of Colorectal Cancer Mortality Attributable to High Processed Meat Intake in China and a Bayesian Projection from 2020 to 2030: A Model-Based Study
Authors: Chen Fangyao , Chen Shiyu , Luo Yaqi , Si Aima , Yang Yuhui , Li Yemian , Hu Weiwei , Zhang Yuxiang
Published: August 2022

Colorectal cancer is among the leading causes of cancer worldwide. Processed meat was known to be positively associated with a higher risk of gastrointestinal cancer. This study focused on the long-time trends of colorectal cancer mortality attributable to high processed meat intake in China from 1990 to 2019 and the projection for the next decade based on data obtained from the Global Burden of Disease 2019 study. We used an age-period-cohort model to fit the long-time trend. The joinpoint model was conducted to estimate the average and annual change of the attributable mortality. The Bayesian age-period-cohort model was used to project the crude attributable mortality from 2020 to 2030. An upward trend in colorectal cancer mortality attributable to high processed meat intake was observed for both sexes in China from 1990 to 2019, with an overall net drift of 4.009% for males and 2.491% for females per year. Projection analysis suggested that the burden of colorectal cancer incidence and mortality would still be high. Our findings suggested that colorectal cancer death attributable to high processed meat intake is still high in China, and elderly males were at higher risk. Gradually decreasing the intake of processed meat could be an effective way to reduce colorectal cancer mortality.
Digital Transformation on Enterprise Green Innovation: Effect and Transmission Mechanism
Authors: Feng Hua , Wang Fengyan , Song Guomin , Liu Lanlan
Published: August 2022

With the development of blockchain, big data, cloud computing and other new technologies, how to achieve innovative development and green sustainable development in digital transformation has become one of the key issues for enterprises to obtain and maintain core competitiveness. However, little of the literature has paid attention to the impact of digital transformation on enterprise green innovation. Using the data of Chinese A-share listed companies from 2010 to 2020, this paper empirically analyzes the impact of enterprise digital transformation on green innovation and its transmission mechanism, by constructing double fixed-effect models. The results show that digital transformation has remarkably promoted the green innovation of enterprises. R&D investment, government subsidies, and income tax burden have played a conductive role between digital transformation and enterprise green innovation. Furthermore, digital transformation can significantly promote the high-quality green innovation of enterprises and also plays a more significant role in promoting the green innovation of high-tech enterprises and state-owned enterprises. A robustness test is carried out by using the lag data and changing the measurement methods of the dependent variable and independent variables, and the research conclusions are still valid. Based on resource-based theory and dynamic capability theory, this paper reveals the impact path of digital transformation on enterprise green innovation, further expanding the research field of digital transformation and enriching the research on the influencing factors of enterprise green innovation. This paper provides policy suggestions for the government to improve the enterprise green innovation level by increasing government subsidies and providing tax incentives and also provides reference for digital transformation enterprises to accelerate green innovation by increasing R&D investment, obtaining government subsidies, and acquiring tax policy support.
KDM2A and KDM3B as Potential Targets for the Rescue of F508del-CFTR
Authors: D’Amore Claudio , Borgo Christian , Bosello Travain Valentina , Salvi Mauro
Published: August 2022

Cystic fibrosis (CF) is caused by mutations in the gene encoding of the cystic fibrosis transmembrane conductance regulator (CFTR), an anion-selective plasma membrane channel that mainly regulates chloride transport in a variety of epithelia. More than 2000 mutations, most of which presumed to be disease-relevant, have been identified in the CFTR gene. The single CFTR mutation F508del (deletion of phenylalanine in position 508) is present in about 90% of global CF patients in at least one allele. F508del is responsible for the defective folding and processing of CFTR, failing to traffic to the plasma membrane and undergoing premature degradation via the ubiquitin–proteasome system. CFTR is subjected to different post-translational modifications (PTMs), and the possibility to modulate these PTMs has been suggested as a potential therapeutic strategy for the functional recovery of the disease-associated mutants. Recently, the PTM mapping of CFTR has identified some lysine residues that may undergo methylation or ubiquitination, suggesting a competition between these two PTMs. Our work hypothesis moves from the idea that favors methylation over ubiquitination, e.g., inhibiting demethylation could be a successful strategy for preventing the premature degradation of unstable CFTR mutants. Here, by using a siRNA library against all the human demethylases, we identified the enzymes whose downregulation increases F508del-CFTR stability and channel function. Our results show that KDM2A and KDM3B downregulation increases the stability of F508del-CFTR and boosts the functional rescue of the channel induced by CFTR correctors.
Performance Analysis of a HT-PEMFC System with 6FPBI Membranes Doped with Cross-Linkable Polymeric Ionic Liquid
Authors: Li Yanju , Shao Wei , Ma Zheshu , Zheng Meng , Song Hanlin
Published: August 2022

In this paper, a high-temperature proton-exchange membrane fuel cell (HT-PEMFC) system using fluorine-containing polybenzimidazole (6FPBI) composite membranes doped with cross-linkable polymer ionic liquid (cPIL) is developed and studied. The reliability of the model is verified by a comparison with the experimental data. The performance of the HT-PEMFC system using 6FPBI membranes with different levels of cPIL is analyzed. The results show that when the HT-PEMFC uses 6FPBI membranes with a cPIL content of 20 wt % (6FPBI-cPIL 20 membranes), the single cell power density is 4952.3 W·m−2. The excessive cPIL content will lead to HT-PEMFC performance degradation. The HT-PEMFC system using the 6FPBI-cPIL 20 membranes shows a higher performance, even at higher temperatures and pressures, than the systems using 6FPBI membranes. In addition, the parametric study results suggest that the HT-PEMFC system should be operated at a higher inlet temperature and hydrogen pressure to increase system output power and efficiency. The oxygen inlet pressure should be reduced to decrease the power consumption of the ancillary equipment and improve system efficiency. The proposed model can provide a prediction for the performance of HT-PEMFC systems with the application of phosphoric-acid-doped polybenzimidazole (PA-PBI) membranes.
Mechanical Stretch Induced Skin Regeneration: Molecular and Cellular Mechanism in Skin Soft Tissue Expansion
Authors: Guo Yaotao , Song Yajuan , Xiong Shaoheng , Wang Tong , Liu Wei , Yu Zhou , Ma Xianjie
Published: August 2022

Skin soft tissue expansion is one of the most basic and commonly used techniques in plastic surgery to obtain excess skin for a variety of medical uses. However, skin soft tissue expansion is faced with many problems, such as long treatment process, poor skin quality, high retraction rate, and complications. Therefore, a deeper understanding of the mechanisms of skin soft tissue expansion is needed. The key to skin soft tissue expansion lies in the mechanical stretch applied to the skin by an inflatable expander. Mechanical stimulation activates multiple signaling pathways through cellular adhesion molecules and regulates gene expression profiles in cells. Meanwhile, various types of cells contribute to skin expansion, including keratinocytes, dermal fibroblasts, and mesenchymal stem cells, which are also regulated by mechanical stretch. This article reviews the molecular and cellular mechanisms of skin regeneration induced by mechanical stretch during skin soft tissue expansion.
Bis-Cyclic Guanidine Heterocyclic Peptidomimetics as Opioid Ligands with Mixed μ-, κ- and δ-Opioid Receptor Interactions: A Potential Approach to Novel Analgesics
Authors: McLaughlin Jay P. , Rayala Ramanjaneyulu , Bunnell Ashley J. , Tantak Mukund P. , Eans Shainnel O. , Nefzi Khadija , Ganno Michelle L. , Dooley Colette T. , Nefzi Adel
Published: August 2022

The design and development of analgesics with mixed-opioid receptor interactions has been reported to decrease side effects, minimizing respiratory depression and reinforcing properties to generate safer analgesic therapeutics. We synthesized bis-cyclic guanidine heterocyclic peptidomimetics from reduced tripeptides. In vitro screening with radioligand competition binding assays demonstrated variable affinity for the mu-opioid receptor (MOR), delta-opioid receptor (DOR), and kappa-opioid receptor (KOR) across the series, with compound 1968-22 displaying good affinity for all three receptors. Central intracerebroventricular (i.c.v.) administration of 1968-22 produced dose-dependent, opioid receptor-mediated antinociception in the mouse 55 °C warm-water tail-withdrawal assay, and 1968-22 also produced significant antinociception up to 80 min after oral administration (10 mg/kg, p.o.). Compound 1968-22 was detected in the brain 5 min after intravenous administration and was shown to be stable in the blood for at least 30 min. Central administration of 1968-22 did not produce significant respiratory depression, locomotor effects or conditioned place preference or aversion. The data suggest these bis-cyclic guanidine heterocyclic peptidomimetics with multifunctional opioid receptor activity may hold potential as new analgesics with fewer liabilities of use.
Uncovering Oncogenic Mechanisms of Tumor Suppressor Genes in Breast Cancer Multi-Omics Data
Authors: Cho Seong Beom
Published: August 2022

Tumor suppressor genes (TSGs) are essential genes in the development of cancer. While they have many roles in normal cells, mutation and dysregulation of the TSGs result in aberrant molecular processes in cancer cells. Therefore, understanding TSGs and their roles in the oncogenic process is crucial for prevention and treatment of cancer. In this research, multi-omics breast cancer data were used to identify molecular mechanisms of TSGs in breast cancer. Differentially expressed genes and differentially coexpressed genes were identified in four large-scale transcriptomics data from public repositories and multi-omics data analyses of copy number, methylation and gene expression were performed. The results of the analyses were integrated using enrichment analysis and meta-analysis of a p-value summation method. The integrative analysis revealed that TSGs have a significant relationship with genes of gene ontology terms that are related to cell cycle, genome stability, RNA processing and metastasis, indicating the regulatory mechanisms of TSGs on cancer cells. The analysis frame and research results will provide valuable information for the further identification of TSGs in different types of cancers.
The Role of Blood-Derived Factors in Protection and Regeneration of Aged Tissues
Authors: Höving Anna L. , Schmidt Kazuko E. , Kaltschmidt Barbara , Kaltschmidt Christian , Knabbe Cornelius
Published: August 2022

Tissue regeneration substantially relies on the functionality of tissue-resident endogenous adult stem cell populations. However, during aging, a progressive decline in organ function and regenerative capacities impedes endogenous repair processes. Especially the adult human heart is considered as an organ with generally low regenerative capacities. Interestingly, beneficial effects of systemic factors carried by young blood have been described in diverse organs including the heart, brain and skeletal muscle of the murine system. Thus, the interest in young blood or blood components as potential therapeutic agents to target age-associated malignancies led to a wide range of preclinical and clinical research. However, the translation of promising results from the murine to the human system remains difficult. Likewise, the establishment of adequate cellular models could help to study the effects of human blood plasma on the regeneration of human tissues and particularly the heart. Facing this challenge, this review describes the current knowledge of blood plasma-mediated protection and regeneration of aging tissues. The current status of preclinical and clinical research examining blood borne factors that act in stem cell-based tissue maintenance and regeneration is summarized. Further, examples of cellular model systems for a more detailed examination of selected regulatory pathways are presented.
Formulation of Magneto-Responsive Hydrogels from Dually Cross-Linked Polysaccharides: Synthesis, Tuning and Evaluation of Rheological Properties
Authors: Vítková Lenka , Musilová Lenka , Achbergerová Eva , Kolařík Roman , Mrlík Miroslav , Korpasová Kateřina , Mahelová Leona , Capáková Zdenka , Mráček Aleš
Published: August 2022

Smart hydrogels based on natural polymers present an opportunity to fabricate responsive scaffolds that provide an immediate and reversible reaction to a given stimulus. Modulation of mechanical characteristics is especially interesting in myocyte cultivation, and can be achieved by magnetically controlled stiffening. Here, hyaluronan hydrogels with carbonyl iron particles as a magnetic filler are prepared in a low-toxicity process. Desired mechanical behaviour is achieved using a combination of two cross-linking routes—dynamic Schiff base linkages and ionic cross-linking. We found that gelation time is greatly affected by polymer chain conformation. This factor can surpass the influence of the number of reactive sites, shortening gelation from 5 h to 20 min. Ionic cross-linking efficiency increased with the number of carboxyl groups and led to the storage modulus reaching 103 Pa compared to 101 Pa–102 Pa for gels cross-linked with only Schiff bases. Furthermore, the ability of magnetic particles to induce significant stiffening of the hydrogel through the magnetorheological effect is confirmed, as a 103-times higher storage modulus is achieved in an external magnetic field of 842 kA·m−1. Finally, cytotoxicity testing confirms the ability to produce hydrogels that provide over 75% relative cell viability. Therefore, dual cross-linked hyaluronan-based magneto-responsive hydrogels present a potential material for on-demand mechanically tunable scaffolds usable in myocyte cultivation.
The Association of Erythropoietin and Age-Related Macular Degeneration in Hemodialysis Patients: A Nationwide Population-Based Cohort Study
Authors: Huang Evelyn-Jou-Chen , Sung Fung-Chang , Hung Peir-Haur , Muo Chih-Hsin , Wu Meei-Maan , Yeh Chih-Ching
Published: August 2022

This population-based retrospective cohort study investigated the effectiveness of erythropoietin (EPO) treatment in reducing the risk of age-related macular degeneration (AMD) in hemodialysis patients, using the National Health Insurance Research Data of Taiwan. From the database, we identified 147,318 end-stage renal disease (ESRD) patients on hemodialysis who had been diagnosed in 2000–2014 to establish the propensity-score-matched EPO user cohort and non-EPO user cohort with equal sample size of 15,992. By the end of 2016, the cumulative incidence of AMD in EPO users was about 3.29% lower than that in non-EPO users (Kaplan–Meier survival p < 0.0001). The risk of AMD was 43% lower in EPO users than in non-EPO users, with an adjusted hazard ratio (aHR) of 0.57 (95% confidence interval (CI) = 0.51–0.64) estimated in the multivariate Cox model. A significant negative dose–response relationship was identified between the EPO dosage and the risk of AMD (p < 0.0001). Another beneficial effect of EPO treatment was a reduced risk of both exudative AMD (aHR = 0.48, 95% CI = 0.40–0.61) and non-exudative AMD (aHR = 0.61, 95% CI = 0.53–0.69), also in similar dose–response relationships (p < 0.0001). Our findings suggest that EPO treatment for hemodialysis patients could reduce AMD risk in a dose–response relationship.
Non-Thermal Ammonia Decomposition for Hydrogen Production over Carbon Films under Low-Temperature Plasma—In-Situ FTIR Studies
Authors: Moszczyńska Julia , Liu Xinying , Wiśniewski Marek
Published: August 2022

Due to easy storage and transportation, liquid hydrogen carriers will play a significant role in diversifying the energy supply pathways by transporting hydrogen on a large scale. Thus, in this study, amorphous carbonaceous materials have been employed for hydrogen production via ammonia decomposition under non-thermal plasma (NTP) conditions. The adsorption and splitting of ammonia over carbons differing in the chemical structure of surface functional groups have been investigated by in situ spectral studies directly under NTP conditions. As a result of NH3 physical and chemical sorption, surface species in the form of ammonium salts, amide and imide structures decompose immediately after switching on the plasma environment, and new functionalities are formed. Carbon catalysts are very active for NH3 splitting. The determined selectivity to H2 is close to 100% on N-doped carbon material. The data obtained indicate that the tested materials possess excellent catalytic ability for economical, COx-free hydrogen production from NH3 at a low temperature.