bluuebunny/crossref_metadata_2025 huggingface dataset:
Fabrication of a Novel Antifouling Polysulfone Membrane with in Situ Embedment of Mxene Nanosheets
Authors: Shen Zhen , Chen Wei , Xu Hang , Yang Wen , Kong Qing , Wang Ao , Ding Mingmei , Shang Juan
Published: November 2019

Membrane fouling is still a critical issue for the application of ultrafiltration, which has been widely used in water treatment due to its efficiency and simplicity. In order to improve the antifouling property, a new 2D material MXene was used to fabricate composite ultrafiltration membrane with the approach of in situ embedment during the phase inversion process in this study. Scanning electron microscopy (SEM), atomic force microscopy (AFM), thermogravimetric analysis (TGA), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), water contact angle, bovine serum albumin rejection and porosity measurements were utilized to characterize the prepared membranes. Due to the hydrophilicity of the MXene, the composite membranes obtained higher hydrophilicity, confirmed by the decreased water contact angle. All the modified membranes had a high bovine serum albumin rejection above 90% while that of the pristine polysulfone membrane was 77.48%. The flux recovery ratio and the reversible fouling ratio of the membranes were also improved along with the increasing content of the MXene. Furthermore, the highest flux recovery ratio could also reach 76.1%. These indicated the good antifouling properties of MXene composite membranes. The enhanced water permeability and protein rejection and excellent antifouling properties make MXene a promising material for antifouling membrane modification.
The Heterogeneous Effects of Different Environmental Policy Instruments on Green Technology Innovation
Authors: Yi Ming , Fang Xiaomeng , Wen Le , Guang Fengtao , Zhang Yao
Published: November 2019

Environmental regulation is an important driving force of green technology innovation. In this paper, environmental policy instruments are classified into three categories: command-control, market-incentive and social-will. Based on the panel data of 30 provinces in China from 2010 to 2017, a fixed effect model and a panel threshold regression model are used to test the heterogeneous effects of different types of environmental policy instruments on the green technology innovation in China. The results show that: (1) Overall, China’s environmental policy instruments do not provide sufficient impetus for green technology innovation; (2) The impact of command-control environmental policy instruments on green technology innovation has a single threshold effect. When its intensity exceeds a certain threshold, green technology innovation is improved. The impact of market-incentive environmental policy instruments on the green technology innovation shows a double threshold effect, that is to say, only when its intensity maintained within a reasonable interval, can green technology innovation be promoted by it; (3) There is significant spatial difference in the impact of different types of environmental policy instruments on green technology innovation. In order to induce green technology innovation, it is necessary to formulate a combined and differentiated environmental policy system, while rationally adjusting the strength of different types of environmental policy instruments.
E-Liquid Flavor Preferences and Individual Factors Related to Vaping: A Survey among Dutch Never-Users, Smokers, Dual Users, and Exclusive Vapers
Authors: Romijnders Kim AGJ , Krüsemann Erna JZ , Boesveldt Sanne , de Graaf Kees , de Vries Hein , Talhout Reinskje
Published: November 2019

Appealing product characteristics, such as flavors, may stimulate e-cigarette use. While switching to e-cigarettes may reduce harm for smokers, concerns exist about e-cigarette use among never-smokers. The role of flavors in the decision to switch to or refrain from vaping is unclear. This study used a bottom–up approach to investigate the relation between flavor preferences and individual factors related to vaping between various user groups. A cross-sectional survey was conducted among never-users (n = 407), smokers (n = 138), dual users (n = 122), and exclusive vapers (n = 61) in the Netherlands. Demographics, attractiveness of product characteristics, flavor preferences, and individual factors related to vaping (knowledge, trust, perceived susceptibility, attitude, social influence, deliberation, and intention) were assessed. The availability of different flavors was the most attractive characteristic of e-cigarettes. Dual users and exclusive vapers had most often used tobacco and menthol/mint flavors when they first started vaping. Compared to dual users, exclusive vapers currently used more fruit and sweet flavors. Never-users who were interested in trying an e-liquid flavor had more knowledge about and a more positive attitude towards e-cigarettes. Smokers who were interested in trying a flavor had a more positive attitude towards e-cigarettes and experienced the social influence towards not using e-cigarettes as less strong than those who did not want to try any flavor. Hence, individual factors related to vaping differed depending on whether never-users and smokers wanted to try an e-liquid flavor. This means that flavors may moderate differences found in individual factors related to vaping, or vice versa.
Removal of Total Nitrogen and Phosphorus Using Single or Combinations of Aquatic Plants
Authors: Su Feng , Li Zhian , Li Yingwen , Xu Lei , Li Yongxing , Li Shiyu , Chen Hongfeng , Zhuang Ping , Wang Faguo
Published: November 2019

Phytoremediation is a potentially suitable technology for taking up large amounts of N and P during plant growth and the removal of plant material, thereby avoiding eutrophication. We compared the capacity of nine different aquatic plant species for removing total P (TP), total N (TN), and NH4+-N from raw domestic sewage wastewater collected from a living area located in Guangzhou city, China, and different concentrations of artificial wastewater. The experiments were performed in two stages, namely screening and modification. In the screening stage, four plant species were identified from the nine grown in raw domestic sewage water for 36 days. In the modification stage, the TN and TP removal ability of different plant combinations were determined in artificial wastewater at different N/P concentrations. After having been grown in monocultures for 46 days, Ipomoea aquatica (90.6% and 8.8%) and Salvinia natans (67.3% and 14.2%) obtained the highest TP removal efficiency in lightly and highly polluted wastewater, respectively. The combination of S. natans and Eleocharis plantagineiformis effectively removed TP and TN from lightly polluted water, suggesting that this combination is suitable for phytoremediation of eutrophic wastewater.
The Expression of BNP, ET-1, and TGF-β1 in Myocardium of Rats with Ventricular Arrhythmias
Authors: Tian Meihui , Xiao Ying , Xue Jiajia , Zhang Yuan , Jia Yuqing , Luo Xinyi , Wang Tianqi , Zhu Baoli , Cao Zhipeng
Published: November 2019

Ventricular arrhythmia (VA) is a major component of sudden cardiac death (SCD). To investigate the expression of brain natriuretic peptide (BNP), endothelin-1 (ET-1), and transforming growth factor-beta 1 (TGF-β1) during VA, we established a rat model of VA induced by BaCl2 solution through a microinjector pump. PD142893 (ET-1 receptor blocker) and SB431542 (TGF-β1 receptor type I blocker) were used to explore the effect of ET-1 and TGF-β1 on BNP expression in the myocardium after VA. BNP, ET-1, and TGF-β1 in rat myocardium were assayed by western blot and immunohistochemical staining for proteins, and real-time quantitative polymerase chain reaction for mRNAs. We found increased expression of BNP and ET-1 in rat myocardium that was associated with the duration of VA. However, TGF-β1 protein expression remained unchanged. Such early increases in BNP and ET-1 may be attributed to fatal arrhythmias associated with SCD, suggesting these may be novel biomarkers of this disease. After intraperitoneal injection of PD142893 and SB431542, respectively, BNP was downregulated in the myocardium of the left ventricle; however, this was abrogated by co-application of the two inhibitors. These results suggested that both ET-1 and TGF-β1, by specifically binding to their receptors, might be involved in the myocardial synthesis of BNP during VA in vivo.
Endoplasmic Reticulum Stress Cooperates in Silica Nanoparticles-Induced Macrophage Apoptosis via Activation of CHOP-Mediated Apoptotic Signaling Pathway
Authors: Chen Fenglei , Jin Jiaqi , Hu Jiahui , Wang Yujing , Ma Zhiyu , Zhang Jinlong
Published: November 2019

While silica nanoparticles (SiNPs) have wide applications, they inevitably increase atmospheric particulate matter and human exposure to this nanomaterial. Numerous studies have focused on how to disclose SiNP toxicity and on understanding its toxic mechanisms. However, there are few studies in the literature reporting the interaction between endoplasmic reticulum (ER) stress and SiNP exposure, and the corresponding detailed mechanisms have not been clearly determined. In this study, CCK-8 and flow cytometry assays demonstrated that SiNPs gradually decreased cell viability and increased cell apoptosis in RAW 264.7 macrophage cells in dose- and time-dependent manners. Western blot analysis showed that SiNPs significantly activated ER stress by upregulating GRP78, CHOP, and ERO1α expression. Meanwhile, western blot analysis also showed that SiNPs activated the mitochondrial-mediated apoptotic signaling pathway by upregulating BAD and Caspase-3, and downregulating the BCL-2/BAX ratio. Moreover, 4-phenylbutyrate (4-PBA), an ER stress inhibitor, significantly decreased GRP78, CHOP, and ERO1α expression, and inhibited cell apoptosis in RAW 264.7 macrophage cells. Furthermore, overexpression of CHOP significantly enhanced cell apoptosis, while knockdown of CHOP significantly protected RAW 264.7 macrophage cells from apoptosis induced by SiNPs. We found that the CHOP-ERO1α-caspase-dependent apoptotic signaling pathway was activated by upregulating the downstream target protein ERO1α and caspase-dependent mitochondrial-mediated apoptotic signaling pathway by upregulating Caspase-3 and downregulating the ratio of BCL-2/BAX. In summary, ER stress participated in cell apoptosis induced by SiNPs and CHOP regulated SiNP-induced cell apoptosis, at least partly, via activation of the CHOP-ERO1α-caspase apoptotic signaling pathway in RAW 264.7 macrophage cells.
Broad-Spectrum Amino Acid Transporters ClAAP3 and ClAAP6 Expressed in Watermelon Fruits
Authors: Shi Tianran , Joshi Vijay , Joshi Madhumita , Vitha Stanislav , Gibbs Holly , Wang Kehua , Okumoto Sakiko
Published: November 2019

Watermelon fruit contains a high percentage of amino acid citrulline (Cit) and arginine (Arg). Cit and Arg accumulation in watermelon fruit are most likely mediated by both de novo synthesis from other amino acids within fruits and direct import from source tissues (leaves) through the phloem. The amino acid transporters involved in the import of Cit, Arg, and their precursors into developing fruits of watermelon have not been reported. In this study, we have compiled the list of putative amino acid transporters in watermelon and characterized transporters that are expressed in the early stage of fruit development. Using the yeast complementation study, we characterized ClAAP3 (Cla023187) and ClAAP6 (Cla023090) as functional amino acid transporters belonging to the family of amino acid permease (AAP) genes. The yeast growth and uptake assays of radiolabeled amino acid suggested that ClAAP3 and ClAAP6 can transport a broad spectrum of amino acids. Expression of translational fusion proteins with a GFP reporter in Nicotiana benthamiana leaves confirmed the ER- and plasma membrane-specific localization, suggesting the role of ClAAP proteins in the cellular import of amino acids. Based on the gene expression profiles and functional characterization, ClAAP3 and ClAAP6 are expected to play a major role in regulation of amino acid import into developing watermelon fruits.
Transferrin Receptor 1-Associated Iron Accumulation and Oxidative Stress Provides a Way for Grass Carp to Fight against Reovirus Infection
Authors: Wan Quanyuan , Liao Zhiwei , Rao Youliang , Yang Chunrong , Ji Jianfei , Chen Xiaohui , Su Jianguo
Published: November 2019

Iron is an essential element, closely linked with host immune responses. Nevertheless, the relationship between iron metabolism and virus infection is still unclear in aquatic vertebrates. To address this issue, we employed grass carp (Ctenopharyngodon idella) and its lethal virus, grass carp reovirus (GCRV), a double-strand RNA virus, as models. Our results demonstrate that GCRV infection increases the iron content and alters the expression of iron metabolism-related genes both in vivo and in vitro. Of note, the expression of C. idella transferrin receptor 1 (CiTfR1) rather than transferrin is upregulated upon GCRV infection. To clarify the implications of CiTfR1 upregulation for antiviral immunity, we proved that CiTfR1 was not a helper for GCRV invasion, but instead, it inhibited GCRV infection and promoted cell proliferation by facilitating the accumulation of intracellular labile iron pool (LIP), which increases intracellular oxidative stress. Interestingly, we found that CiTfR1 overexpression inhibited the mRNA expression of C. idella interferon 1 (CiIFN1) and CiIFN3. The present study reveals a novel antiviral defense mechanism in teleost where TfR1 induces the accumulation of LIP, leading to the suppression of virus infection and the proliferation of host cells, indicating that iron can be used as a medicated feed additive for the control of animal viral disease.
Genome-Wide Analysis of the YABBY Transcription Factor Family in Pineapple and Functional Identification of AcYABBY4 Involvement in Salt Stress
Authors: Li Zeyun , Li Gang , Cai Mingxing , Priyadarshani Samaranayaka V.G.N. , Aslam Mohammad , Zhou Qiao , Huang Xiaoyi , Wang Xiaomei , Liu Yeqiang , Qin Yuan
Published: November 2019

The plant-specific transcription factor gene family, YABBY, belongs to the subfamily of zinc finger protein superfamily and plays an essential regulatory role in lateral organ development. In this study, nine YABBY genes were identified in the pineapple genome. Seven of them were located on seven different chromosomes and the remaining two were located on scaffold 1235. Through protein structure prediction and protein multiple sequence alignment, we found that AcYABBY3, AcYABBY5 and AcYABBY7 lack a C2 structure in their N-terminal C2C2 zinc finger protein structure. Analysis of the cis-acting element indicated that all the seven pineapple YABBY genes contain multiple MYB and MYC elements. Further, the expression patterns analysis using the RNA-seq data of different pineapple tissues indicated that different AcYABBYs are preferentially expressed in various tissues. RT-qPCR showed that the expression of AcYABBY2, AcYABBY3, AcYABBY6 and AcYABBY7 were highly sensitive to abiotic stresses. Subcellular localization in pineapple protoplasts, tobacco leaves and Arabidopsis roots showed that all the seven pineapple YABBY proteins were nucleus localized. Overexpression of AcYABBY4 in Arabidopsis resulted in short root under NaCl treatment, indicating a negative regulatory role of AcYABBY4 in plant resistance to salt stress. This study provides valuable information for the classification of pineapple AcYABBY genes and established a basis for further research on the functions of AcYABBY proteins in plant development and environmental stress response.
Osteoporosis in Rheumatic Diseases
Authors: Adami Giovanni , Fassio Angelo , Rossini Maurizio , Caimmi Cristian , Giollo Alessandro , Orsolini Giovanni , Viapiana Ombretta , Gatti Davide
Published: November 2019

Osteoporosis is a chronic disease characterized by an increased risk of fragility fracture. Patients affected by rheumatic diseases are at greater risk of developing osteoporosis. The purpose of the present review is to discuss the pathogenesis, epidemiology, and treatment of osteoporosis in patients affected by rheumatic diseases with special focus for rheumatoid arthritis, psoriatic arthritis, spondyloarthritis, systemic lupus erythematosus, systemic sclerosis, vasculitides, Sjogren syndrome, and crystal-induced arthritis.
Enhanced Neuroprotective Effects of Panax ginseng G115® and Ginkgo biloba GK501® Combinations In Vitro Models of Excitotoxicity
Authors: Landucci Elisa , Pellegrini-Giampietro Domenico E. , Bilia Anna Rita , Bergonzi Maria Camilla
Published: November 2019

Neurological-related disorders are seen as an increasingly important aspect of welfare. While conventional medicine is still the mainstay for the treatment of these diseases, it is becoming apparent that patients are also seeking more natural and preventative interventions. Panax ginseng G115® and Ginkgo biloba GK501® extracts alone or in combination were used in two in vitro experimental models of primary cultures exposed to excitotoxicity: rat organotypic hippocampal slices exposed to either 5 µM kainic acid or 10 µM N-Methyl-d-aspartate for 24 hours, and mixed cortical cells exposed to 300 µM NMDA for 10 min. Cell death in the Cornu Ammonis areas CA3 or CA1 subregions of slices was quantified by measuring propidium iodide fluorescence, whereas in cortical cells, it was assessed by measuring the amount of lactate dehydrogenase. In slices, treatment with extracts alone or in combination significantly attenuated CA3 and CA1 damage induced by exposure to kainic acid or NMDA, respectively. A similar neuroprotective effect was observed in cortical cells exposed to NMDA. Analysis of cell signaling pathways found that the two extracts induced an increase of the phosphorylation and they reversed the decrease of phosphorylation of ERK1/2 and Akt induced by kainic acid and NMDA in organotypic hippocampal slices. These results suggest that P. ginseng G115® and G. biloba GK501® extracts may mediate their effects by activating phosphorylation of ERK1/2 and Akt signaling pathways, protecting against excitotoxicity-induced damage in in vitro models.
Comparison of Renal Function Estimation Formulae for Dosing Direct Oral Anticoagulants in Patients with Atrial Fibrillation
Authors: Lee Kwang-No , Choi Jong-Il , Kim Yun Gi , Boo Ki Yung , Kim Do Young , Choi Yun Young , Choi Ha Young , Kim Dong-Hyeok , Lee Dae In , Roh Seung-Young , Shim Jaemin , Kim Jin Seok , Kim Young-Hoon
Published: November 2019

The Cockcroft-Gault (CG) formula is recommended to guide clinicians in the choice of the appropriate dosage for direct oral anticoagulants (DOACs). However, the performance of the CG formula varies depending on the patient’s age, weight, and degree of renal function. We aimed to compare the validity of the CG formula with that of Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) and Modification of Diet in Renal Disease (MDRD) formulae for dosing DOACs. A total of 6268 consecutive patients on anticoagulants for atrial fibrillation (AF) were retrospectively investigated. Among underweight and elderly patients, the CG formula underestimated renal function compared with the non-CG formulae. However, the concordant rate of drug indications between the CG and the non-CG formulae was approximately 94%. On-label uses under the three formulae were associated with a lower risk of major bleeding (but not thromboembolism) compared to warfarin. Although we found differences in estimating renal function and the proportions of drug indications between the CG and non-CG formulae, the risks of thromboembolism and major bleeding were similar to those with warfarin regardless of which formula was used.
Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation
Authors: Pagano Sabrina , Magenta Alessandra , D’Agostino Marco , Martino Francesco , Barillà Francesco , Satta Nathalie , Frias Miguel A. , Ronca Annalisa , Mach François , Gencer Baris , Favari Elda , Vuilleumier Nicolas
Published: November 2019

Aims: Anti-Apolipoprotein A-1 autoantibodies (anti-ApoA-1 IgG) promote atherogenesis via innate immune receptors, and may impair cellular cholesterol homeostasis (CH). We explored the presence of anti-ApoA-1 IgG in children (5–15 years old) with or without familial hypercholesterolemia (FH), analyzing their association with lipid profiles, and studied their in vitro effects on foam cell formation, gene regulation, and their functional impact on cholesterol passive diffusion (PD). Methods: Anti-ApoA-1 IgG and lipid profiles were measured on 29 FH and 25 healthy children. The impact of anti-ApoA-1 IgG on key CH regulators (SREBP2, HMGCR, LDL-R, ABCA1, and miR-33a) and foam cell formation detected by Oil Red O staining were assessed using human monocyte-derived macrophages. PD experiments were performed using a validated THP-1 macrophage model. Results: Prevalence of high anti-ApoA-1 IgG levels (seropositivity) was about 38% in both study groups. FH children seropositive for anti-ApoA-1 IgG had significant lower total cholesterol LDL and miR-33a levels than those who were seronegative. On macrophages, anti-ApoA-1 IgG induced foam cell formation in a toll-like receptor (TLR) 2/4-dependent manner, accompanied by NF-kB- and AP1-dependent increases of SREBP-2, LDL-R, and HMGCR. Despite increased ABCA1 and decreased mature miR-33a expression, the increased ACAT activity decreased membrane free cholesterol, functionally culminating to PD inhibition. Conclusions: Anti-ApoA-1 IgG seropositivity is frequent in children, unrelated to FH, and paradoxically associated with a favorable lipid profile. In vitro, anti-ApoA-1 IgG induced foam cell formation through a complex interplay between innate immune receptors and key cholesterol homeostasis regulators, functionally impairing the PD cholesterol efflux capacity of macrophages.
Deciphering the Biological Mechanisms Underlying the Genome-Wide Associations between Computerized Device Use and Psychiatric Disorders
Authors: Wendt Frank R , Muniz Carvalho Carolina , Pathak Gita A. , Gelernter Joel , Polimanti Renato
Published: November 2019

Computerized device use (CDU) is societally ubiquitous but its effects on mental health are unknown. We performed genetic correlation, Mendelian randomization, and latent causal variable analyses to identify shared genetic mechanisms between psychiatric disorders (Psychiatric Genomics Consortium; 14,477 < N < 150,064) and CDU (UK Biobank; N = 361,194 individuals). Using linkage disequilibrium score regression, we detected strong genetic correlations between “weekly usage of mobile phone in last 3 months” (PhoneUse) vs. attention deficit hyperactivity disorder (ADHD; rg = 0.425, p = 4.59 × 10−29) and “plays computer games” (CompGaming) vs. schizophrenia (SCZ; rg = −0.271, p = 7.16 × 10−26). Focusing on these correlations, we used two sample MRs to detect the causal relationships between trait pairs by treating single nucleotide polymorphisms as non-modifiable risk factors underlying both phenotypes. Significant bidirectional associations were detected (PhoneUse→ADHD β = 0.132, p = 1.89 × 10−4 and ADHD→PhoneUse β = 0.084, p = 2.86 × 10−10; CompGaming→SCZ β = −0.02, p = 6.46 × 10−25 and CompGaming→SCZ β = −0.194, p = 0.005) and the latent causal variable analyses did not support a causal relationship independent of the genetic correlations between these traits. This suggests that molecular pathways contribute to the genetic overlap between these traits. Dopamine transport enrichment (Gene Ontology:0015872, pSCZvsCompGaming = 2.74 × 10−10) and DRD2 association (pSCZ = 7.94 × 10−8; pCompGaming = 3.98 × 10−25) were detected in SCZ and CompGaming and support their negative correlative relationship. FOXP2 was significantly associated with ADHD (p = 9.32 × 10−7) and PhoneUse (p = 9.00 × 10−11) with effect directions concordant with their positive genetic correlation. Our study demonstrates that epidemiological associations between psychiatric disorders and CDUs are due, in part, to the molecular mechanisms shared between them rather than a causal relationship. Our findings imply that biological mechanisms underlying CDU contribute to the psychiatric phenotype manifestation.
Ca2+ Flux: Searching for a Role in Efferocytosis of Apoptotic Cells in Atherosclerosis
Authors: Tajbakhsh Amir , Kovanen Petri T. , Rezaee Mahdi , Banach Maciej , Sahebkar Amirhossein
Published: November 2019

In atherosclerosis, macrophages in the arterial wall ingest plasma lipoprotein-derived lipids and become lipid-filled foam cells with a limited lifespan. Thus, efficient removal of apoptotic foam cells by efferocytic macrophages is vital to preventing the dying foam cells from forming a large necrotic lipid core, which, otherwise, would render the atherosclerotic plaque vulnerable to rupture and would cause clinical complications. Ca2+ plays a role in macrophage migration, survival, and foam cell generation. Importantly, in efferocytic macrophages, Ca2+ induces actin polymerization, thereby promoting the formation of a phagocytic cup necessary for efferocytosis. Moreover, in the efferocytic macrophages, Ca2+ enhances the secretion of anti-inflammatory cytokines. Various Ca2+ antagonists have been seminal for the demonstration of the role of Ca2+ in the multiple steps of efferocytosis by macrophages. Moreover, in vitro and in vivo experiments and clinical investigations have revealed the capability of Ca2+ antagonists in attenuating the development of atherosclerotic plaques by interfering with the deposition of lipids in macrophages and by reducing plaque calcification. However, the regulation of cellular Ca2+ fluxes in the processes of efferocytic clearance of apoptotic foam cells and in the extracellular calcification in atherosclerosis remains unknown. Here, we attempted to unravel the molecular links between Ca2+ and efferocytosis in atherosclerosis and to evaluate cellular Ca2+ fluxes as potential treatment targets in atherosclerotic cardiovascular diseases.
Statin-Induced Nitric Oxide Signaling: Mechanisms and Therapeutic Implications
Authors: Gorabi Armita , Kiaie Nasim , Hajighasemi Saeideh , Banach Maciej , Penson Peter , Jamialahmadi Tannaz , Sahebkar Amirhossein
Published: November 2019

In addition to their cholesterol-lowering effects, statins are associated with pleiotropic effects including improvements in heart failure (HF), reduced blood pressure, prevention of the rupture of atherosclerotic plaques and improved angiogenesis. In addition to these cardiovascular benefits, statins have been implicated in the treatment of neurological injuries, cancer, sepsis, and cirrhosis. These cholesterol-independent beneficial effects of statins are predominantly mediated through signaling pathways leading to increased production and bioavailability of nitric oxide (NO). In this review, the mechanistic pathways and therapeutic effects of statin-mediated elevations of NO are described and discussed.
Trends and Disparities in Self-Reported and Measured Osteoporosis among US Adults, 2007–2014
Authors: Wu Qing , Xu Yingke , Lin Ge
Published: November 2019

(1) Background: Studies examining osteoporosis trends among US adults by different socioeconomic status (SES) are limited. The prevalence of self-reported osteoporosis in the US is rarely reported. (2) Methods: Data from the National Health and Nutritional Examination Survey (NHANES) between 2007–2008 and 2013–2014 cycles were analyzed. Age-adjusted prevalence of self-reported and that of measured osteoporosis were calculated overall and by sex, race/ethnicity, education attainment, and SES. (3) Results: The prevalence of self-reported osteoporosis was higher than that of measured osteoporosis in all three survey cycles for women, and in 2007–2008 and 2009–2010 for men. Participants with high school/GED or higher educational attainment had an increased prevalence of measured osteoporosis during the study period. Among all SES groups, participants with low family income (PIR < 1.3) had the highest prevalence of measured osteoporosis, and the prevalence increased from 49.3 per 1000 population to 71.8 per 1000 population during the study period. (4) Conclusions: The prevalence of self-reported osteoporosis was higher than that of measured osteoporosis in US adults between 2007 and 2014. The age-adjusted prevalence of measured osteoporosis increased in participants with the educational attainment of high school/GED or above, and individuals with low family income.
Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer’s Disease
Authors: Moon Gyeong Joon , Kim Sehwan , Jeon Min-Tae , Lee Kea Joo , Jang Il-Sung , Nakamura Michiko , Kim Sang Ryong
Published: November 2019

We recently reported that adeno-associated virus serotype 1-constitutively active Ras homolog enriched in brain [AAV1-Rheb(S16H)] transduction of hippocampal neurons could induce neuron-astroglia interactions in the rat hippocampus in vivo, resulting in neuroprotection. However, it remains uncertain whether AAV1-Rheb(S16H) transduction induces neurotrophic effects and preserves the cognitive memory in an animal model of Alzheimer’s disease (AD) with characteristic phenotypic features, such as β-amyloid (Aβ) accumulation and cognitive impairments. To assess the therapeutic potential of Rheb(S16H) in AD, we have examined the beneficial effects of AAV1-Rheb(S16H) administration in the 5XFAD mouse model. Rheb(S16H) transduction of hippocampal neurons in the 5XFAD mice increased the levels of neurotrophic signaling molecules, including brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF), and their corresponding receptors, tropomyosin receptor kinase B (TrkB) and CNTF receptor α subunit (CNTFRα), respectively. In addition, Rheb(S16H) transduction inhibited Aβ production and accumulation in the hippocampus of 5XFAD mice and protected the decline of long-term potentiation (LTP), resulting in the prevention of cognitive impairments, which was demonstrated using novel object recognition testing. These results indicate that Rheb(S16H) transduction of hippocampal neurons may have therapeutic potential in AD by inhibiting Aβ accumulation and preserving LTP associated with cognitive memory.
Influence of EEDI (Energy Efficiency Design Index) on Ship–Engine–Propeller Matching
Authors: Ren Huilin , Ding Yu , Sui Congbiao
Published: November 2019

With the increasingly strict international GHG (greenhouse gas) emission regulations, higher requirements are placed on the propulsion system design of conventional ships. Playing an important role in ship design, construction and operation, ship–engine–propeller matching dominantly covers the CO2 emission of the entire ship. In this paper, firstly, a ship propulsion system matching platform based on the ship–engine–propeller matching principle and its application on WinGD 5X52 marine diesel engine have been investigated. Meeting the energy efficiency design index (EEDI) regulation used to calculate the ship CO2 emission is essential and ship–engine–propeller matching has to be carried out with EEDI into consideration. Consequently, a procedure is developed combining the system matching theory and EEDI calculation, which can provide the matching results as well as the corresponding EEDI value to study the relationship between EEDI and ship–engine–propeller matching. Furthermore, a comprehensive analysis is performed to obtain the relationship of EEDI and system matching parameters, such as ship speed, effective power and propeller diameter, reflecting the trend and extent of EEDI when changing these three parameters. The results of system matching parameters satisfying different EEDI phases indicate the initial value selection in matching process to provide reference for the design of ship, engine and propeller under the EEDI regulations.
Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review
Authors: Dannaoui Eric , Espinel-Ingroff Ana
Published: November 2019

Antifungal susceptibility testing is an important tool for managing patients with invasive fungal infections, as well as for epidemiological surveillance of emerging resistance. For routine testing in clinical microbiology laboratories, ready-to-use commercial methods are more practical than homemade reference techniques. Among commercially available methods, the concentration gradient Etest strip technique is widely used. It combines an agar-based diffusion method with a dilution method that determinates a minimal inhibitory concentration (MIC) in µg/mL. Many studies have evaluated the agreement between the gradient strip method and the reference methods for both yeasts and filamentous fungi. This agreement has been variable depending on the antifungal, the species, and the incubation time. It has also been shown that the gradient strip method could be a valuable alternative for detection of emerging resistance (non-wild-type isolates) as Etest epidemiological cutoff values have been recently defined for several drug-species combinations. Furthermore, the Etest could be useful for direct antifungal susceptibility testing on blood samples and basic research studies (e.g., the evaluation of the in vitro activity of antifungal combinations). This review summarizes the available data on the performance and potential use of the gradient strip method.
The Burden of Fungal Infections in Ethiopia
Authors: Tufa Tafese B. , Denning David W.
Published: November 2019

The burden of severe fungal infections (FIs) is not well addressed in Ethiopia. We have estimated the burden of FIs from multiple demographic sources and by searching articles from PubMed. Opportunistic FIs were estimated using modelling and 2017 national HIV data. The burdens of chronic pulmonary aspergillosis (CPA) and allergic bronchopulmonary aspergillosis (ABPA) were estimated by using the prevalence of asthma, chronic obstructive pulmonary disease, and annual the incidence of tuberculosis. Of the 105,000,000 estimated Ethiopian population, 610,000 are thought to have HIV infection. Our estimation of HIV-related FIs were: 9900 cryptococcal meningitis (CM), 12,700 Pneumocystis jirovecii pneumonia (PCP), 76,300 oral and 56,000 oesophageal candidiasis cases. A remarkable 7,051,700 4–14-year-olds probably have tinea capitis and 1,469,000 women probably have recurrent Candida vaginitis. There were 15,200 estimated CPA cases (prevalence) and 11,500 invasive aspergillosis (IA) cases (incidence). Data are scant, but we estimated 5300 candidaemia and 800 Candida peritonitis cases. In conclusion, approximately 8% of Ethiopians suffer from FIs annually, mostly schoolchildren with tinea capitis. IA, CM and PCP are the major causes of fungal deaths. The absence of CD4 count is challenging the identification of HIV patients at risk of opportunistic FIs. There is a pressing need to improve FI diagnosis, probably including national surveillance.
Coupling Coordinated Development and Exploring Its Influencing Factors in Nanchang, China: From the Perspectives of Land Urbanization and Population Urbanization
Authors: Lv Tiangui , Wang Li , Zhang Xinmin , Xie Hualin , Lu Hua , Li Hongyi , Liu Wangda , Zhang Yanwei
Published: November 2019

The coordination relationship between land urbanization and population urbanization is crucial for achieving sustainable development under economic transition. Moreover, the balance between land urbanization and population urbanization is essential to guarantee the urbanization process of an entire city. This paper empirically analyzes the interaction between land urbanization and population urbanization in Nanchang from 2002 to 2017 based on the coupling coordination model (CCM). The impacts of the coordination degree on coordinated development are quantified by multivariate linear regression (MLR). The results show the following: (1) The indices of land urbanization and population urbanization in Nanchang showed an upward trend, and therefore the coordination degree in Nanchang increased significantly from 2002 to 2017. (2) The coordinated development of urbanization underwent two stages: disordered and moderately disordered. (3) The urban population proportion and the supporting capability of agricultural production had a positive impact on coordinated development. Meanwhile, the results also show that per capita education expenditures and the per capita public green area had negative impacts on the coordination degree, while economic development and the urban industrial structure were positive contributors to the coordination degree. Finally, this paper proposes that policies should be formulated to achieve coordinated development of urbanization. It can be concluded that the results regarding coordinated development of urbanization can help decision makers formulate effective measures to achieve coordinated development in the future.
Effect of Dispersion by Three-Roll Milling on Electrical Properties and Filler Length of Carbon Nanotube Composites
Authors: Ha Ji-Hwan , Lee Sang-Eui , Park Sung-Hoon
Published: November 2019

For practical use of carbon nanotube (CNT) composites, especially in electronic applications, uniform dispersion of a high concentration of CNTs in a polymer matrix is a critical challenge. Three-roll milling is one of most reliable dispersion techniques. We investigate the effect of three-roll milling time on CNT length and the electrical properties of a CNT/polydimethylsiloxane composite film with 10 wt% CNTs. During the milling process, the CNT length is decreased from 10 to 1–4 μm by mechanical shear forces. The electrical conductivity increases after 1.5 min of milling owing to dispersion of the CNTs but decreases with increasing milling time owing to the decrease in the CNT length. Considering the changes in the electrical conductivity of the CNT composite and CNT length, we determined how to optimize the three-roll milling time to obtain a suitable dispersion state.
Broadening the Photoluminescence Excitation Spectral Bandwidth of YVO4:Eu3+ Nanoparticles via a Novel Core-Shell and Hybridization Approach
Authors: Huang Jianhua , Tang Lu , Chen Nan , Du Guoping
Published: November 2019

For many optoelectronic applications, it is desirable for the lanthanide-doped phosphors to have broad excitation spectrum. The excitation mechanism of the lanthanide-doped YVO4, a high quantum efficient lasing material, primarily originates from the energy transfer process from the host VO43− complexes to the lanthanide ions, which has an excitation spectral bandwidth range of 230–330 nm. For applications in silicon solar cells, such phosphors can convert ultraviolet light to visible light for more efficient power generation, but this spectral range is still not broad enough to cover the entire ultraviolet spectrum of solar light. In this work, a novel core-shell and inorganic–organic hybridization strategy has been employed to fabricate Eu3+-doped YVO4 nanoparticles to broaden their photoluminescence excitation spectral bandwidth to the range of 230–415 nm, covering the entire ultraviolet spectrum of solar light and enabling their potential applications in silicon solar cells.
Influence of Print Orientation on Surface Roughness in Fused Deposition Modeling (FDM) Processes
Authors: Buj-Corral Irene , Domínguez-Fernández Alejandro , Durán-Llucià Ramón
Published: November 2019

In the present paper, we address the influence of print orientation angle on surface roughness obtained in lateral walls in fused deposition modelling (FDM) processes. A geometrical model is defined that considers the shape of the filaments after deposition, in order to define a theoretical roughness profile, for a certain print orientation angle. Different angles were considered between 5° and 85°. Simulated arithmetical mean height of the roughness profile, Ra values, were calculated from the simulated profiles. The Ra simulated results were compared to the experimental results, which were carried out with cylindrical PLA (polylactic acid) samples. The simulated Ra values were similar to the experimental values, except for high angles above 80°, where experimental roughness decreased while simulated roughness was still high. Low print orientation angles show regular profiles with rounded peaks and sharp values. At a print orientation angle of 85°, the shape of the profile changes with respect to lower angles, showing a gap between adjacent peaks. At 90°, both simulated and experimental roughness values would be close to zero, because the measurement direction is parallel to the layer orientation. Other roughness parameters were also measured: maximum height of profile, Rz, kurtosis, Rku, skewness, Rsk, and mean width of the profile elements, Rsm. At high print orientation angles, Rz decreases, Rku shifts to positive, Rsk slightly increases, and Rsk decreases, showing the change in the shape of the roughness profiles.
Burst Release of Antibiotics Combined with Long-Term Release of Silver Targeting Implant-Associated Infections: Design, Characterization and in vitro Evaluation of Novel Implant Hybrid Surface
Authors: Borcherding Kai , Marx Dennis , Gätjen Linda , Bormann Nicole , Wildemann Britt , Specht Uwe , Salz Dirk , Thiel Karsten , Grunwald Ingo
Published: November 2019

Implant-associated infections represent a serious risk in human medicine and can lead to complications, revisions and in worst cases, amputations. To target these risks, the objective was to design a hybrid implant surface that allows a local burst release of antibiotics combined with long-term antimicrobial activity based on silver. The efficacy should be generated with simultaneous in vitro cytocompatibility. The investigations were performed on titanium K-wires and plates and gentamicin was selected as an illustrative antibiotic. A gentamicin depot (max 553 µg/cm2) was created on the surface using laser structuring. The antibiotic was released within 15 min in phosphate buffered saline (PBS) or agar medium. Metallic silver particles (4 µg/cm2) in a titanium dioxide layer were deposited using plasma vapor deposition (PVD). About 16% of the silver was released within 28 days in the agar medium. The local efficacy of the incorporated silver was demonstrated in a direct contact assay with a reduction of more than 99.99% (Escherichia coli). The local efficacy of the hybrid surface was confirmed in a zone of inhibition (ZOI) assay using Staphylococcus cohnii. The biocompatibility of the hybrid surface was proven using fibroblasts and osteoblasts as cell systems. The hybrid surface design seems to be promising as treatment of implant-associated infections, considering the achieved amount and release behavior of the active ingredients (gentamicin, silver). The generated in vitro results (efficacy, biocompatibility) proofed the concept. Further in vivo studies will be necessary translate the hybrid surface towards clinical applied research.
Synthesis of Sub 3 nm-Sized Uniform Magnetite Nanoparticles Using Reverse Micelle Method for Biomedical Application
Authors: Jung Euiyoung , Kim Sung-Won , Cho Ahyoung , Kim Yu-Jin , Jeong Gun-Jae , Kim Jinheung , Bhang Suk Ho , Yu Taekyung
Published: November 2019

We report a synthetic method for small and uniform Fe3O4 (magnetite) nanoparticles under mild conditions. Spherical sub-3 nm-sized magnetite nanoparticles were prepared via reverse micelles composed of oleylamine, F127, xylene, and water for the reaction of iron(III) stearate with hydrazine at a reaction temperature of 90 °C in air atmosphere. These synthesized magnetite nanoparticles exhibited good size uniformity. By controlling experimental conditions, we could easily control both size and size uniformity of these magnetite nanoparticles. We further investigated whether Fe3O4 could be used in biomedical applications. Cytotoxicity of Fe3O4 was evaluated with human adipose-derived stem cells (hADSCs). Our results showed that the number of hADSCs did not significantly decrease when these cells were treated with Fe3O4 nanoparticles at a concentration of up to 9 μg/mL. Apoptotic activity and cell proliferation of hADSCs treated with Fe3O4 nanoparticles were similar to those of hADSCs without any treatment. This novel method could be used for synthesizing uniform and biocompatible Fe3O4 nanoparticles with further biomedical applications.
Sustainable Lubrication Methods for the Machining of Titanium Alloys: An Overview
Authors: García-Martínez Enrique , Miguel Valentín , Martínez-Martínez Alberto , Manjabacas María Carmen , Coello Juana
Published: November 2019

Titanium is one of the most interesting materials in modern manufacturing thanks to its good mechanical properties and light weight. These features make it very attractive for use in the aeronautical and aerospace industries. Important alloys, such as Ti6Al4V, are extensively used. Nevertheless, titanium alloys present several problems in machining processes. Their machinability is poor, affected by low thermal conductivity, which generates very high cutting temperatures and thermal gradients in the cutting tool. Lubricants and cutting fluids have traditionally been used to solve this problem. However, this option is unsustainable as such lubricants represent a risk to the environment and to the health of the operator due to their different chemical components. Therefore, novel, sustainable and green lubrication techniques are necessary. Dry machining is the most sustainable option. Nevertheless, difficult-to-machine materials like titanium alloys cannot be machined under these conditions, leading to very high cutting temperatures and excessive tool wear. This study is intended to describe, analyse and review the non-traditional lubrication techniques developed in turning, drilling and milling processes since 2015, including minimum quantity of lubricant, cryogenic lubrication, minimum quantity of cooling lubrication or high-pressure coolant. The aim is to provide a general overview of the recent advances in each technique for the main machining processes.
Study of Hybrid Nanoparticles Modified Epoxy Resin Used in Filament Winding Composite
Authors: Di Chengrui , Yu Junwei , Wang Baoming , Lau Alan Kin Tak , Zhu Bo , Qiao Kun
Published: November 2019

Hybrid nanoparticles modified bisphenol A type epoxy/acid anhydride resin system applicable for filament winding forming process was studied using elastic core-shell rubber (CSR) nanoparticles with a large particle size (nearly 100 nm) and rigid nano-SiO2 particles with a small particle size (about 16 nm). The formulation, process properties, mechanical properties, thermal properties and microstructure of modified resin and its wound composite were studied. The results suggested that at the content of 10 phr CSR and 2 phr nano-SiO2, the resin system achieved optimum comprehensive performance. The viscosity of modified resin system was nearly 1000 mPa·s at 25 °C and service life was over 6 h. The resin tensile strength and modulus were 89 MPa and 3.5 GPa, while flexural strength and modulus reached 128 MPa and 3.2 GPa, respectively. The impact strength was 26.6 kJ·m−2, and the glass transition temperature (Tg) reached 145.9 °C. Modified epoxy resin enhanced the mechanical properties of carbon fiber reinforced wound composite. The tensile strength, tensile modulus and interlaminar shear strength were enhanced by 14.0%, 4.56% and 18.9%, respectively, compared with a composite based on unmodified resin. The above test results and scanning electron microscopy (SEM) analysis suggest that the hybrid nanoparticles modified resin system was suitable for carbon fiber wet filament winding products.