bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Value-Added Compound Recovery with High-Temperature Hydrothermal Treatment and Steam Explosion, and Subsequent Biomethanization of Residual Strawberry Extrudate
Authors: Cubero-Cardoso Juan , Trujillo-Reyes Ángeles , Serrano Antonio , Rodríguez-Gutiérrez Guillermo , Borja Rafael , Fermoso Fernando G.
Published: August 2020

This study was on the comparison of hydrothermal treatments at 170 °C (steam injection) and 220 °C (steam explosion) to solubilize the organic matter contained in residual strawberry extrudate, focusing on phenolic compounds that were susceptible to be extracted and on sugars. After the extraction step, the remaining strawberry extrudate phases were subjected to anaerobic digestion to generate biogas that would compensate the energy requirements of the suggested hydrothermal treatments and to stabilize the remaining waste. Hydrothermal treatment at 220 °C allowed the recovery of 2053 mg of gallic acid eq. per kg of residual strawberry extrudate. By contrast, after hydrothermal treatment at 170 °C, only 394 mg of gallic acid eq. per kg of residual strawberry extrudate was recovered. Anaerobic digestion processes were applied to the de-phenolized liquid phase and the solid phase together, which generated similar methane productions, i.e., around 430 mL CH4/g volatile solids, after both 170 °C and 220 °C hydrothermal treatments. Considering the latest observation, hydrothermal treatment at 220 °C is a preferable option for the valorization of residual strawberry extrudate (RSE) due to the high solubilization of valuable phenolic compounds that can be recovered.
Hierarchical Structures and Leadership Design in Mean-Field-Type Games with Polynomial Cost
Authors: El Oula Frihi Zahrate , Barreiro-Gomez Julian , Eddine Choutri Salah , Tembine Hamidou
Published: August 2020

This article presents a class of hierarchical mean-field-type games with multiple layers and non-quadratic polynomial costs. The decision-makers act in sequential order with informational differences. We first examine the single-layer case where each decision-maker does not have the information about the other control strategies. We derive the Nash mean-field-type equilibrium and cost in a linear state-and-mean-field feedback form by using a partial integro-differential system. Then, we examine the Stackelberg two-layer problem with multiple leaders and multiple followers. Numerical illustrations show that, in the symmetric case, having only one leader is not necessarily optimal for the total sum cost. Having too many leaders may also be suboptimal for the total sum cost. The methodology is extended to multi-level hierarchical systems. It is shown that the order of the play plays a key role in the total performance of the system. We also identify a specific range of parameters for which the Nash equilibrium coincides with the hierarchical solution independently of the number of layers and the order of play. In the heterogeneous case, it is shown that the total cost is significantly affected by the design of the hierarchical structure of the problem.
Temporal and Spectral Variability of OJ 287 before the April–June 2020 Outburst
Authors: Kalita Nibedita , Gupta Alok C. , Gu Minfeng
Published: August 2020

We present the results of a temporal and spectral study of the BL Lacertae object OJ 287 in optical, UV, and X-ray bands with observations performed by Swift satellite during September 2019–March 2020. In this period, the source showed moderate variability characterized by variability amplitude of ∼22–31% in all the wavelengths on a short timescale, except the hard X-ray band which was variable by only ∼8%. We observed that the X-ray flux of the source was significantly dominated by the soft photons below 2 keV. Soft lags of ∼45 days were detected between the optical/UV and soft X-ray emissions, while there is no correlation between the hard X-rays and the lower energy bands indicating the presence of two emission components or electron populations. Although two components contribute to the X-ray emission, most of the 0.3–10 keV spectra were well fitted with an absorbed power-law model which outlines the dominance of synchrotron over inverse Compton (IC) mechanism. The X-ray spectra follow a weak “softer when brighter” trend.
Integration of QTL, Transcriptome and Polymorphism Studies Reveals Candidate Genes for Water Stress Response in Tomato
Authors: Diouf Isidore , Albert Elise , Duboscq Renaud , Santoni Sylvain , Bitton Frédérique , Gricourt Justine , Causse Mathilde
Published: August 2020

Water deficit (WD) leads to significant phenotypic changes in crops resulting from complex stress regulation mechanisms involving responses at the physiological, biochemical and molecular levels. Tomato growth and fruit quality have been shown to be significantly affected by WD stress. Understanding the molecular mechanism underlying response to WD is crucial to develop tomato cultivars with relatively high performance under low watering conditions. Transcriptome response to WD was investigated through the RNA sequencing of fruit and leaves in eight accessions grown under two irrigation conditions, in order to get insight into the complex genetic regulation of WD response in tomato. Significant differences in genotype WD response were first observed at the phenotypic level for fruit composition and plant development traits. At the transcriptome level, a total of 14,065 differentially expressed genes (DEGs) in response to WD were detected, among which 7393 (53%) and 11,059 (79%) were genotype- and organ-specific, respectively. Water deficit induced transcriptome variations much stronger in leaves than in fruit. A significant effect of the genetic background on expression variation was observed compared to the WD effect, along with the presence of a set of genes showing a significant genotype x watering regime interaction. Integrating the DEGs with previously identified WD response quantitative trait loci (QTLs) mapped in a multi-parental population derived from the crossing of the eight genotypes narrowed the candidate gene lists to within the confidence intervals surrounding the QTLs. The results present valuable resources for further study to decipher the genetic determinants of tomato response to WD.
Anaphase Bridges: Not All Natural Fibers Are Healthy
Authors: Finardi Alice , Massari Lucia F. , Visintin Rosella
Published: August 2020

At each round of cell division, the DNA must be correctly duplicated and distributed between the two daughter cells to maintain genome identity. In order to achieve proper chromosome replication and segregation, sister chromatids must be recognized as such and kept together until their separation. This process of cohesion is mainly achieved through proteinaceous linkages of cohesin complexes, which are loaded on the sister chromatids as they are generated during S phase. Cohesion between sister chromatids must be fully removed at anaphase to allow chromosome segregation. Other (non-proteinaceous) sources of cohesion between sister chromatids consist of DNA linkages or sister chromatid intertwines. DNA linkages are a natural consequence of DNA replication, but must be timely resolved before chromosome segregation to avoid the arising of DNA lesions and genome instability, a hallmark of cancer development. As complete resolution of sister chromatid intertwines only occurs during chromosome segregation, it is not clear whether DNA linkages that persist in mitosis are simply an unwanted leftover or whether they have a functional role. In this review, we provide an overview of DNA linkages between sister chromatids, from their origin to their resolution, and we discuss the consequences of a failure in their detection and processing and speculate on their potential role.
Sex-Specific Transcriptome Differences in Human Adipose Mesenchymal Stem Cells
Authors: Bianconi Eva , Casadei Raffaella , Frabetti Flavia , Ventura Carlo , Facchin Federica , Canaider Silvia
Published: August 2020

In humans, sexual dimorphism can manifest in many ways and it is widely studied in several knowledge fields. It is increasing the evidence that also cells differ according to sex, a correlation still little studied and poorly considered when cells are used in scientific research. Specifically, our interest is on the sex-related dimorphism on the human mesenchymal stem cells (hMSCs) transcriptome. A systematic meta-analysis of hMSC microarrays was performed by using the Transcriptome Mapper (TRAM) software. This bioinformatic tool was used to integrate and normalize datasets from multiple sources and allowed us to highlight chromosomal segments and genes differently expressed in hMSCs derived from adipose tissue (hADSCs) of male and female donors. Chromosomal segments and differentially expressed genes in male and female hADSCs resulted to be related to several processes as inflammation, adipogenic and neurogenic differentiation and cell communication. Obtained results lead us to hypothesize that the donor sex of hADSCs is a variable influencing a wide range of stem cell biologic processes. We believe that it should be considered in biologic research and stem cell therapy.
Identifying Gaps in the Investigation of the Vredefort Granophyre Dikes: A Systematic Literature Review
Authors: Huber Matthew S. , Kovaleva Elizaveta
Published: August 2020

The Vredefort impact structure is among the oldest and largest impact structures preserved on Earth. An understanding of its key features can serve as a guide for learning about the development of basin-sized impact structures on Earth and other planetary bodies. One of these features is the so-called Vredefort granophyre dikes, which formed when molten material from the impact melt sheet was emplaced below the crater floor. The importance of these dikes has been recognized since the earliest studies of the Vredefort structure, nearly 100 years ago. The present study is a systematic literature review to determine the extent to which peer-reviewed scientific publications have generated unique data regarding the granophyre dikes and to investigate how scientific methods used to investigate the granophyre have changed over time. In total, 33 unique studies have been identified. Of those, more studies have been performed into the core-collar dikes than the core dikes. The majority of the studies have focused on field analyses, bulk geochemistry, and the studies of mineral components. The granophyre has long been recognized as a product of post-deformational processes and thus has been a target of age dating to constrain the minimum age of the impact event. In the last 25 years, studies of stable isotopes and shock deformation of minerals in lithic clasts within the dikes have taken place. A small number of geophysical studies relevant to the granophyre dikes have also been undertaken. Overall, there has been a relatively small number of studies on this important rock type, and the studies that have taken place tend to focus on two particular dikes. Several of the dikes have only been investigated by regional studies and have not been specifically targeted. The use of modern techniques has been lacking. More fieldwork, as well as geophysical, isotopic, microstructural studies, and application of novel techniques, are necessary for the granophyre dikes to be truly understood.
Responding to Maternal Loss: A Phenomenological Study of Older Orphans in Youth-Headed Households in Impoverished Areas of South Africa
Authors: Ntuli Busisiwe , Sebola Ephodia , Madiba Sphiwe
Published: August 2020

The aim of this study was to explore how older orphans in youth-headed households (YHHs) experience and respond to maternal death and to examine the strategies they employ to care for their younger siblings. We interviewed 18 older orphans who were purposively selected from YHHs located in informal settlements in the City of Tshwane, South Africa. After the death of their mothers, the orphans lost the family home, lost support from their relatives, lost friendships, lost educational opportunities, and lost childhood. The orphans experienced prolonged pain, sadness, anxieties, fear, loneliness despondency, and deep-rooted and persistent anger towards their mother for dying. They suffered from prolonged bereavement because they had been denied the opportunity to mourn the loss of their parents and yearned persistently but silently for their dead mothers. Dropping out of school to seek employment in order to care for their siblings was one of the main coping strategies that older orphans used. However, dropping out of school early robbed them of their future goals of getting an educational qualification. The orphans had not been prepared for taking on an adult role and were given no support or counselling to help them recover from their parents’ death. Continuous grief counselling should form an integral component of the psychosocial support services that are provided to orphans soon after the death of a parent.
To Develop Health Education Tools for Nasogastric Tube Home Caring Through Participatory Action Research
Authors: Lin Fang-Suey , Shi Hong-Chun
Published: August 2020

Medical institutions provide guidance on caring skills for home caregivers. Oral teaching is combined with graphical tools in a method that has been proved to be an effective way of quickly mastering home caring skills and promotes effective learning for home caregivers. The graphic design and operation contents of this method are constantly revised through interviews and observations, and by carrying out home care application graphics it forms a spiral structure of Plan–Do–Study–Act (PDSA) participatory action research (PAR). In the three cycles of the operation of PDSA PAR, the designers accurately create graphics of the caring details based on the nurses’ demonstrations and develop health education tools that are suitable to provide continuous assistance and services in real-life situations. PAR combined with PDSA, in each of the three cycles of the operation—design personnel, medical personnel and home caregiver personnel, respectively—as the lead roles, guide the planning decisions for PAR. This study is a reference for the improvement and development of medical graphics for health education tools to improve accuracy.
Oral Health and Care for Elderly People with Alzheimer’s Disease
Authors: Gao Sherry Shiqian , Chu Chun Hung , Young Fanny Yuk Fun
Published: August 2020

Dementia is one of the main causes of disability among elderly people. It is a progressive neurodegenerative disease that affects elderly people’s ability to perform daily living activities. Alzheimer’s disease is the main subtype of dementia and causes declining memory, reasoning, and communication skills. They also have behavioural and psychological symptoms, such as depression and aggression. It is essential for them to maintain good oral health, as oral health is an important and integral part of their general health. Neglecting oral health allows dental diseases to develop, and these diseases are difficult and costly to treat. However, dental diseases can be treated with ambulatory care rather than hospitalisation and emergency care. Elderly people should establish daily oral hygiene care routines during the early stages of Alzheimer’s disease. They should have regular dental examinations and early minimal interventions to prevent the need for extensive and complicated procedures. Maintaining oral health becomes challenging, however, when Alzheimer’s disease progresses to the middle and late stages. Because elderly people might forget or lose interest in keeping their teeth healthy, caretakers and community health workers may need to take over this task. Dentists should provide guidance on the maintenance of oral health, as the techniques used to provide this support vary depending on the elderly people concerned. The purpose of this paper is to provide an overview of oral health and the importance of oral care for elderly people with Alzheimer’s disease. The paper also discusses appropriate dental interventions and techniques for maintaining good oral health and helping people with Alzheimer’s to enjoy a satisfactory quality of life.
Feasibility of 3-Dimensional Visual Guides for Preparing Pediatric Zirconia Crowns: An In Vitro Study
Authors: Kang Ho Yeon , Lee Hyeonjong , Chae Yong Kwon , Hong Seoung-Jin , Jeong Yun Yeong , Lee Ko Eun , Kim Mi Sun , Lee Hyo-Seol , Choi Sung Chul , Nam Ok Hyung
Published: August 2020

This study evaluated the feasibility of a tooth preparation guide for prefabricated zirconia crowns (PZCs). Three-dimensional surface data for PZCs of the left maxillary primary first molar and left mandibular primary second molar were obtained using a model scanner. The tooth preparation data were digitally designed to harmonize with the adjacent teeth on the mixed dentition model and visualized using a color-coded map, which presents the required amount of tooth reduction. Twenty participants were recruited for preparing teeth with and without using the tooth preparation guide. The following three parameters were evaluated: tooth preparation time, harmony score, and amount of tooth reduction. The preparation time when using the guide was significantly reduced (p < 0.05), and a significant difference was observed in the harmony scores for the maxillary primary first molar preparation. Furthermore, the amount of tooth reduction was significantly different for both maxillary and mandibular primary molars (p < 0.05) in terms of the occlusal distal surface and buccal line angle in the maxillary primary first molars, and the smooth surfaces, proximal surfaces, and mesial line angles in the mandibular primary second molars. Thus, the results suggest that a tooth preparation guide could facilitate better tooth preparation for PZCs.
An Exploration of U.S. Southern Faith Leaders’ Perspectives of HIV Prevention, Sexuality, and Sexual Health Teachings
Authors: Pichon Latrice C. , Williams Powell Terrinieka , Williams Stubbs Andrea , Becton-Odum Nicole , Ogg Siri , Arnold Trisha , Thurston Idia B.
Published: August 2020

Reducing human immunodeficiency viruses (HIV) and acquired immune deficiency syndrome (AIDS) racial/ethnic disparities in the Deep South has been a critical objective of the U.S. National HIV/AIDS Strategy. This finding, originally published in 2010 by the Office of National AIDS Policy, serves as a complement to the Health and Human Resources and Services Administration’s Ending the HIV Epidemic (EtHE): A Plan for America. The EtHE plan, released in 2019, emphasizes community stakeholder involvement to achieve the planning goals of decreasing new HIV infections in key U.S. geographic areas. According to the plan, an important stakeholder is faith leaders, especially around stigma reduction. This paper focuses on a community–academic research partnership’s exploration of southern Black faith leaders’ teaching perspectives regarding HIV prevention, sexuality, and sexual health in predominantly Black congregations in Memphis, Tennessee. The partnership conducted four focus groups using a semi-structured discussion interview. Any adult faith leader involved in ministry work in a predominantly Black church was eligible to participate in the discussion. A total of 26 faith leaders with a mean age of 54, representing four Christian denominations, consented to participate in the study. Emerging themes included: (1) restriction of scripture to teach prevention and address sexuality, (2) role of secrecy and silence in living with HIV, and (3) impact of the stigma of HIV and sexuality. Findings may inform nationwide jurisdictional implementation plans, particularly for faith-based interventions in southern churches working toward ending the HIV epidemic.
Alternative Methods of Sterilization in Dental Practices Against COVID-19
Authors: Cumbo Enzo , Gallina Giuseppe , Messina Pietro , Scardina Giuseppe Alessandro
Published: August 2020

SARS-CoV-2, and several other microorganisms, may be present in nasopharyngeal and salivary secretions in patients treated in dental practices, so an appropriate clinical behavior is required in order to avoid the dangerous spread of infections. COVID-19 could also be spread when patients touches a contaminated surface with infected droplets and then touch their nose, mouth, or eyes. It is time to consider a dental practice quite similar to a hospital surgery room, where particular attention should be addressed to problems related to the spreading of infections due to air and surface contamination. The effectiveness of conventional cleaning and disinfection procedures may be limited by several factors; first of all, human operator dependence seems to be the weak aspect of all procedures. The improvement of these conventional methods requires the modification of human behavior, which is difficult to achieve and sustain. As alternative sterilization methods, there are some that do not depend on the operator, because they are based on devices that perform the entire procedure on their own, with minimal human intervention. In conclusion, continued efforts to improve the traditional manual disinfection of surfaces are needed, so dentists should consider combining the use of proper disinfectants and no-touch decontamination technologies to improve sterilization procedures.
Changes in Knowledge about Umbilical Cord Blood Banking and Genetic Tests among Pregnant Women from Polish Urban and Rural Areas between 2010–2012 and 2017
Authors: Szubert Maria , Ilowiecka Malwina , Wilczynski Jacek , Szpotanska-Sikorska Monika , Wojtyla Cezary
Published: August 2020

The aim of this study was to evaluate knowledge of umbilical cord blood (UBC) banking and prenatal genetic diagnosis among pregnant women from rural and urban areas, and how this knowledge changed within a five-year period. A survey by questionnaire was conducted between 2010 and 2012, and in 2017 in public hospitals; the study population comprised 6128 women, with 2797 patients from the years 2010–2012 and 3331 from the year 2017. 41% of the studied population declared that they were living in rural areas. In the 2010–2012 period, fewer women from rural areas knew about UBC banking. In 2017 that same relative difference in knowledge persisted, but the percentage of women who now knew about this procedure rose significantly in both studied groups. Prenatal diagnosis was more familiar for urban inhabitants both in 2010 and 2017 but as with the UBC data, a trend of growing awareness was also seen in pregnant women from rural areas. Knowledge of new techniques such as UBC banking and genetic tests has grown among pregnant women during the time frame of our study, but there is still a need to emphasize the benefits of these two possibilities to pregnant women, especially among rural inhabitants.
The Effects of Forgiveness, Gratitude, and Self-Control on Reactive and Proactive Aggression in Bullying
Authors: García-Vázquez Fernanda Inéz , Valdés-Cuervo Angel Alberto , Parra-Pérez Lizeth Guadalupe
Published: August 2020

The social cognitive approach to moral development posits that moral self-schemas encourage character strengths and reduce adolescents’ aggression. However, limited research has examined the influence of positive personal characteristics on proactive behaviors and reactive aggression in bullying. This study examined direct and mediational relationships between forgiveness, gratitude, self-control, and both proactive and reactive aggression in bullying. The extent to which the structural relations of this model were invariant by gender and stage of adolescence were also evaluated. Participants in this study were 1000 Mexican students, 500 early adolescents (M age = 12.36, SD = 0.77 years) and 500 middle adolescents (M age = 16.64, SD = 0.89 years), between 12 and 17 years old. Structural equation and multi-group invariance analysis were performed. Results indicate that gratitude and forgiveness are positively related to self-control. Gratitude, forgiveness, and self-control are also negatively related to reactive and proactive aggression. Forgiveness and gratitude had an indirect relationship by decreasing both proactive and reactive aggression through their positive effects on self-control. Additionally, gender moderated the relationships between variables proposed in the model, whereas stage of adolescence did not. Overall findings suggest that moral self-schemas and strengths explained both types of aggression in bullying.
Association of Reallocating Time in Different Intensities of Physical Activity with Weight Status Changes among Normal-Weight Chinese Children: A National Prospective Study
Authors: Tan Kaiyun , Cai Li , Lai Lijuan , Gui Zhaohuan , Zeng Xia , Lv Yajie , Zhang Jingshu , Wang Hui , Ma Yinghua , Chen Yajun
Published: August 2020

Background: Time spent in different intensity-specific physical activities is codependent, but the substitution effect of different activities on weight status changes in children remains unclear. This study aims to investigate the prospective association between reallocating time in different intensities of physical activity and weight status changes among Chinese children. Methods: A national sample of 15,100 normal-weight children aged 7–18 years (46.7% boys) were recruited in September 2013 and followed up for nine months. Vigorous-intensity physical activity (VPA), moderate-intensity physical activity (MPA), walking, and sedentary time were obtained by International Physical Activity Questionnaire Short Form (IPAQ-SF). Height and weight were objectively measured, by which body mass index (BMI) and BMI z-score were calculated. Weight status was classified by the Chinese criteria for 7- to 18-year-old children. Isotemporal substitution analyses (including single-factor model, partition model, and isotemporal substitution model) were applied to examine the association of time allocation with weight status changes. Results: Each 30 min/day of increase in VPA was favorably associated with a 13.2% reduced risk of incident overweight/obesity in a single-factor model and a 15.6% reduced risk in a partition model. Negative associations were found between VPA, MPA, walking and the risk of being underweight in the single-factor model, but not in the partition model. In substitution models, replacing 30 min/day sedentary time with an equal amount of VPA was favorably associated with a 16.1% reduction of the risk of being overweight/obese. Conclusion: These findings highlight the need for promoting vigorous-intensity physical activity in children.
Pilot Testing of a Nudge-Based Digital Intervention (Welbot) to Improve Sedentary Behaviour and Wellbeing in the Workplace
Authors: Haile Caitlin , Kirk Alison , Cogan Nicola , Janssen Xanne , Gibson Ann-Marie , MacDonald Bradley
Published: August 2020

Welbot is a nudge-based digital intervention that aims to reduce sedentary behaviour and improve physical and mental wellbeing at work. The purpose of this study was to pilot test the Welbot intervention. Forty-one (6M/35F) University staff (M age = 43-years) participated in this study, which was a single arm repeated measures trial conducted over three weeks of intervention. The primary outcome was sedentary behaviour (measured subjectively and objectively) and secondary outcomes included: mental wellbeing, procrastination, depression, anxiety and stress, and work engagement. A subset of participants (n = 6) wore an ActivPAL to objectively measure activity data, while another subset of participants (n = 6) completed a qualitative semi-structured interview to ascertain experiences of using Welbot. Following the intervention, a Friedman non-parametric test revealed that participants self-reported significantly less time sitting and more time standing and objectively recorded more steps at the week-1 follow-up. A series of paired t-tests exhibited that changes in all secondary outcomes were in the expected direction. However, only improvements in depression, anxiety, and stress were significant. After using Welbot, thematic analysis demonstrated that participants perceived they had a positive behaviour change, increased awareness of unhealthy behaviours at work, and provided suggestions for intervention improvement. Overall, findings provided indications of the potential positive impact Welbot may have on employees’ wellbeing, however, limitations are noted. Recommendations for intervention improvement including personalisation (e.g., individual preferences for nudges and the option to sync Welbot with online calendars) and further research into how users engage with Welbot are provided.
A BWM-TOPSIS Hazardous Waste Inventory Safety Risk Evaluation
Authors: Deng Fumin , Li Yanjie , Lin Huirong , Miao Jinrui , Liang Xuedong
Published: August 2020

Hazardous waste can cause severe environmental pollution if not disposed of properly, which in turn can seriously affect the sustainable development of the entire ecology and will inevitably bring disaster to companies. However, because of limited available disposal capacity, it is often difficult to safely dispose of hazardous waste, meaning that it must be kept as passive inventory. For the passive inventory of hazardous waste, risk evaluation of safe operation of the inventory is crucial and urgently needs to be resolved. Based on this, this paper focuses on the risk management of hazardous waste inventory of waste-producing companies and proposes a risk evaluation system for safely dealing with hazardous waste inventory, which expands the scope of inventory safety management and provides guidance to companies on developing appropriate measures to ensure hazardous waste inventory safety. First, the risk evaluation index system for hazardous waste inventory is constructed from equipment, management level, nature of hazardous waste and operational aspects. Then, the best worst method (BWM) is employed to calculate the criteria weights and the technique for order performance by similarity to ideal solution (TOPSIS) is employed to rank the alternatives. Finally, risk evaluation on four waste-producing companies was conducted using the developed method. The results show that Case Company 4 has the greatest risk of hazardous waste inventory, which should be reduced by improving storage method and the amount of hazardous waste. It was found that the proposed evaluation system was effective for hazardous waste inventory safety risk assessments and that the designed index system could assist companies improve their hazardous waste inventory management.
Assessing the Psychometric Properties of the Internet Addiction Test in Peruvian University Students
Authors: Tafur-Mendoza Arnold Alejandro , Acosta-Prado Julio César , Zárate-Torres Rodrigo Arturo , Ramírez-Ospina Duván Emilio
Published: August 2020

The use of the Internet has been gradually and unstoppably gaining ground in all areas of life, from recreational activities to how social relations are established. However, the existence of clinical cases indicates that the addictive use of the Internet is a problem that seriously affects some people. Among the instruments that measure this construct, the Internet Addiction Test (IAT) stands out. However, instrumental studies of this test are scarce in Latin America. The present study sought to analyze the psychometric properties of the IAT in a sample of 227 Peruvian undergraduate university students. Confirmatory factor analysis was used to provide validity evidence based on the internal structure, and evidence based on the relationship with other variables was also provided. Reliability was estimated through the ordinal alpha coefficient. The results indicated that the IAT adequately fits a bifactor model (with two specific factors, time/control and stress/compensate), obtaining good levels of reliability. Additionally, the IAT scores correlate significantly with the average number of hours per day on the internet and social skills. The results lead to the conclusion that the scores in the IAT have evidence of validity and reliability for its use.
Advancing Prevention of STIs by Developing Specific Serodiagnostic Targets: Trichomonas vginalis as a Model
Authors: Alderete John F.
Published: August 2020

Point-of-Care (POC) serum antibody screening of large cohorts of women and men at risk for the sexually transmitted infection (STI) caused by Trichomonas vaginalis requires the availability of targets with high specificity. Such targets should comprise epitopes unique to T. vaginalis immunogenic proteins detected by sera of women and men patients with trichomonosis but not uninfected controls. Three enzymes to which patients make serum IgG antibody were identified as fructose-1,6-bisphosphate aldolase (A), α-enolase (E), and glyceraldehyde-3-phosphate dehydrogenase (G). Epitopes within these proteins were identified that had no sequence identity to enzymes of humans and other pathogens. Therefore, I constructed a chimeric recombinant String-Of-Epitopes (SOE) protein consisting of 15-mer peptides, within which are the epitopes of A, E, and G. This novel protein of ~36-kD is comprised of two epitopes of A, ten epitopes of E, and seven epitopes of G (AEG::SOE2). The AEG::SOE2 protein was detected both by immunoblot and by enzyme-linked immunosorbent assay (ELISA) using highly reactive sera of women and men but not negative serum unreactive to T. vaginalis proteins. Finally, AEG::SOE2 was found to be immunogenic, as evidenced by serum IgG from immunized mice. I discuss how this approach is important in relation to infectious disease diagnostic targets for detection of serum IgG antibody in exposed and/or infected individuals and how such novel targets may have potential as subunit vaccine candidates against microbial pathogens.
Conformity of Annual Reports to an Integrated Reporting Framework: ASE Listed Companies
Authors: Altarawneh Ghada A. , Al-Halalmeh Asma’a Omar
Published: August 2020

The objectives of this study are to determine the level of conformity between Current Issued Reports (CIRs) and Integrated Report (IR) elements of the Amman Stocks Exchange (ASE) listed companies, as well as to determine whether the investigated corporate characteristics (size, age, quality assurance (QA), earning per share (EPS), industry type, foreign ownership (FO)) of these companies have any impact on the conformability of CIRs. It is worth mentioning that (QA), and (EPS), have never been examined by looking at its association with corporate disclosures, and IR in particular. Based on adoption of the IR framework and using the method of content analysis, corporate annual reports and other stand-alone reports of 82 companies in 2017 and 2018 within the financial, industrial, and services sectors, were chosen for this study. The findings of the study provide an answer to the research question and show that sectors vary in their levels of conformity. It reveals that the service sector shows the lowest conformability compared to other sectors, whereas the financial firms conform 65%, followed by the industrial sector. It also finds a positive association between CIRs conformability and variables of size, age of company and quality assurance. However, EPS, FO and type of industry were found to have no impact on the conformability of CIRs to the IR framework. This study has contributed to IR research, which, as a field, has previously received very little recognition among scholars in Jordan. Moreover, IR still does not exist in Jordan’s business practices.
How the AHR Became Important in Intestinal Homeostasis—A Diurnal FICZ/AHR/CYP1A1 Feedback Controls Both Immunity and Immunopathology
Authors: Rannug Agneta
Published: August 2020

Ever since the 1970s, when profound immunosuppression caused by exogenous dioxin-like compounds was first observed, the involvement of the aryl hydrocarbon receptor (AHR) in immunomodulation has been the focus of considerable research interest. Today it is established that activation of this receptor by its high-affinity endogenous ligand, 6-formylindolo[3,2-b]carbazole (FICZ), plays important physiological roles in maintaining epithelial barriers. In the gut lumen, the small amounts of FICZ that are produced from L-tryptophan by microbes are normally degraded rapidly by the inducible cytochrome P4501A1 (CYP1A1) enzyme. This review describes how when the metabolic clearance of FICZ is attenuated by inhibition of CYP1A1, this compound passes through the intestinal epithelium to immune cells in the lamina propria. FICZ, the level of which is thus modulated by this autoregulatory loop involving FICZ itself, the AHR and CYP1A1, plays a central role in maintaining gut homeostasis by potently up-regulating the expression of interleukin 22 (IL-22) by group 3 innate lymphoid cells (ILC3s). IL-22 stimulates various epithelial cells to produce antimicrobial peptides and mucus, thereby both strengthening the epithelial barrier against pathogenic microbes and promoting colonization by beneficial bacteria. Dietary phytochemicals stimulate this process by inhibiting CYP1A1 and causing changes in the composition of the intestinal microbiota. The activity of CYP1A1 can be increased by other microbial products, including the short-chain fatty acids, thereby accelerating clearance of FICZ. In particular, butyrate enhances both the level of the AHR and CYP1A1 activity by stimulating histone acetylation, a process involved in the daily cycle of the FICZ/AHR/CYP1A1 feedback loop. It is now of key interest to examine the potential involvement of FICZ, a major physiological activator of the AHR, in inflammatory disorders and autoimmunity.
Galectin-3, N-terminal Propeptides of Type I and III Procollagen in Patients with Atrial Fibrillation and Metabolic Syndrome
Authors: Ionin Valery A. , Baranova Elena I. , Zaslavskaya Ekaterina L. , Petrishcheva Elena Yu. , Morozov Aleksandr N. , Shlyakhto Evgeny V.
Published: August 2020

The aim of this study was to determine the concentration of galectin-3, PINP and PIIINP in patients with metabolic syndrome (MS) and atrial fibrillation (AF) with an assessment of the relationship with severity of left atrium fibrosis. A total of 480 subjects were included in the case-control study: MS patients (n = 337), 176 of whom had AF, 72 patients with AF without MS and 71 healthy subjects. Galectin-3, PINP and PIIINP blood concentrations and metabolic parameters were compared with the severity of left atrium fibrosis, measured by CARTO3. Galectin-3 in AF and MS patients is higher than in MS without AF and in healthy subjects (10.3 (4.8–15.4), 5.1 (4.3–8.8), 3.2 (2.4–4.2) ng/mL, p < 0.0001). Galectin-3 serum concentration in AF patients with MS is higher than in patients without MS: 10.3 (4.8–15.4), 6.8 (5.2–8.1) ng/mL, p = 0.0001. PINP and PIIINP concentration were higher in patients with AF and MS than in MS without AF: 3499.1 (2299.2–4567.3), 2130.9 (1425.3–2861.8) pg/mL, p < 0.0001, 94.9 (64.8–123.5), 57.6 (40.5–86.9) ng/mL, p < 0.0001. Galectin-3 correlates with PINP (r = 0.496, p < 0.001) and PIIINP concentration (r = 0.451, p < 0.0001). The correlation between galectin-3, PINP and the severity of left atrium fibrosis was found (r = 0.410, p < 0.001; r = 0.623, p < 0.001). Galectin-3 higher than 12.6 ng/mL increased the risk of AF more than five-fold. High galectin-3, PINP and PIIINP concentrations were associated with heart remodeling in MS patients and increased the risk of AF.
Interplay between Carbonic Anhydrases and Metallothioneins: Structural Control of Metalation
Authors: Wong Daisy L. , Yuan Amelia T. , Korkola Natalie C. , Stillman Martin J.
Published: August 2020

Carbonic anhydrases (CAs) and metallothioneins (MTs) are both families of zinc metalloproteins central to life, however, they coordinate and interact with their Zn2+ ion cofactors in completely different ways. CAs and MTs are highly sensitive to the cellular environment and play key roles in maintaining cellular homeostasis. In addition, CAs and MTs have multiple isoforms with differentiated regulation. This review discusses current literature regarding these two families of metalloproteins in carcinogenesis, with a dialogue on the association of these two ubiquitous proteins in vitro in the context of metalation. Metalation of CA by Zn-MT and Cd-MT is described. Evidence for protein–protein interactions is introduced from changes in metalation profiles of MT from electrospray ionization mass spectrometry and the metalation rate from stopped-flow kinetics. The implications on cellular control of pH and metal donation is also discussed in the context of diseased states.
The Cysteine-Rich Repeat Protein TaCRR1 Participates in Defense against Both Rhizoctonia cerealis and Bipolaris sorokiniana in Wheat
Authors: Guo Feilong , Shan Zilong , Yu Jinfeng , Xu Gangbiao , Zhang Zengyan
Published: August 2020

The domain of unknown function 26 (DUF26), harboring a conserved cysteine-rich motif (C-X8-C-X2-C), is unique to land plants. Several cysteine-rich repeat proteins (CRRs), belonging to DUF26-containing proteins, have been implicated in the defense against fungal pathogens in ginkgo, cotton, and maize. However, little is known about the functional roles of CRRs in the important staple crop wheat (Triticum aestivum). In this study, we identified a wheat CRR-encoding gene TaCRR1 through transcriptomic analysis, and dissected the defense role of TaCRR1 against the soil-borne fungi Rhizoctonia cerealis and Bipolaris sorokiniana, causal pathogens of destructive wheat diseases. TaCRR1 transcription was up-regulated in wheat towards B. Sorokiniana or R. cerealis infection. The deduced TaCRR1 protein contained a signal peptide and two DUF26 domains. Heterologously-expressed TaCRR1 protein markedly inhibited the mycelia growth of B. sorokiniana and R. cerealis. Furthermore, the silencing of TaCRR1 both impaired host resistance to B. sorokiniana and R. cerealis and repressed the expression of several pathogenesis-related genes in wheat. These results suggest that the TaCRR1 positively participated in wheat defense against both B. sorokiniana and R. cerealis through its antifungal activity and modulating expression of pathogenesis-related genes. Thus, TaCRR1 is a candidate gene for improving wheat resistance to B. sorokiniana and R. cerealis.
Molecular Insight into the Anti-Inflammatory Effects of the Curcumin Ester Prodrug Curcumin Diglutaric Acid In Vitro and In Vivo
Authors: Phumsuay Rianthong , Muangnoi Chawanphat , Dasuni Wasana Peththa Wadu , Hasriadi Hasriadi , Vajragupta Opa , Rojsitthisak Pornchai , Towiwat Pasarapa
Published: August 2020

Curcumin diglutaric acid (CurDG), an ester prodrug of curcumin, has the potential to be developed as an anti-inflammatory agent due to its improved solubility and stability. In this study, the anti-inflammatory effects of CurDG were evaluated. The effects of CurDG on inflammatory mediators were evaluated in LPS-stimulated RAW 264.7 macrophage cells. CurDG reduced the increased levels of NO, IL-6, and TNF- α, as well as iNOS and COX-2 expression in cells to a greater extent than those of curcumin, along with the potent inhibition of MAPK (ERK1/2, JNK, and p38) activity. The anti-inflammatory effects were assessed in vivo by employing a carrageenan-induced mouse paw edema model. Oral administration of CurDG demonstrated significant anti-inflammatory effects in a dose-dependent manner in mice. The effects were significantly higher compared to those of curcumin at the corresponding doses (p < 0.05). Moreover, 25 mg/kg curcumin did not exert a significant anti-inflammatory effect for the overall time course as indicated by the area under the curve data, while the equimolar dose of CurDG produced significant anti-inflammatory effects comparable with 50, 100, and 200 mg/kg curcumin (p < 0.05). Similarly, CurDG significantly reduced the proinflammatory cytokine expression in paw edema tissues compared to curcumin (p < 0.05). These results provide the first experimental evidence for CurDG as a promising anti-inflammatory agent.
Origin of the Induced Pluripotent Stem Cells Affects Their Differentiation into Dopaminergic Neurons
Authors: Chlebanowska Paula , Sułkowski Maciej , Skrzypek Klaudia , Tejchman Anna , Muszyńska Agata , Noroozi Rezvan , Majka Marcin
Published: August 2020

Neuronal differentiation of human induced pluripotent stem (iPS) cells, both in 2D models and 3D systems in vitro, allows for the study of disease pathomechanisms and the development of novel therapies. To verify if the origin of donor cells used for reprogramming to iPS cells can influence the differentiation abilities of iPS cells, peripheral blood mononuclear cells (PBMC) and keratinocytes were reprogrammed to iPS cells using the Sendai viral vector and were subsequently checked for pluripotency markers and the ability to form teratomas in vivo. Then, iPS cells were differentiated into dopaminergic neurons in 2D and 3D cultures. Both PBMC and keratinocyte-derived iPS cells were similarly reprogrammed to iPS cells, but they displayed differences in gene expression profiles and in teratoma compositions in vivo. During 3D organoid formation, the origin of iPS cells affected the levels of FOXA2 and LMX1A only in the first stages of neural differentiation, whereas in the 2D model, differences were detected at the levels of both early and late neural markers FOXA2, LMX1A, NURR1, TUBB and TH. To conclude, the origin of iPS cells may significantly affect iPS differentiation abilities in teratomas, as well as exerting effects on 2D differentiation into dopaminergic neurons and the early stages of 3D midbrain organoid formation.
Dietary Phenolics against Breast Cancer. A Critical Evidence-Based Review and Future Perspectives
Authors: Ávila-Gálvez María Ángeles , Giménez-Bastida Juan Antonio , Espín Juan Carlos , González-Sarrías Antonio
Published: August 2020

Breast cancer (BC) is the most common malignancy and the leading cause of cancer-related death in adult women worldwide. Over 85% of BC cases are non-hereditary, caused by modifiable extrinsic factors related to lifestyle, including dietary habits, which play a crucial role in cancer prevention. Although many epidemiological and observational studies have inversely correlated the fruit and vegetable consumption with the BC incidence, the involvement of their phenolic content in this correlation remains contradictory. During decades, wrong approaches that did not consider the bioavailability, metabolism, and breast tissue distribution of dietary phenolics persist behind the large currently existing gap between preclinical and clinical research. In the present review, we provide comprehensive preclinical and clinical evidence according to physiologically relevant in vitro and in vivo studies. Some dietary phenolics such as resveratrol (RSV), quercetin, isoflavones, epigallocatechin gallate (EGCG), lignans, and curcumin are gaining attention for their chemopreventive properties in preclinical research. However, the clinical evidence of dietary phenolics as BC chemopreventive compounds is still inconclusive. Therefore, the only way to validate promising preclinical results is to conduct clinical trials in BC patients. In this regard, future perspectives on dietary phenolics and BC research are also critically discussed.
The Precious Few Grams of Glucose During Exercise
Authors: Brooks George A.
Published: August 2020

As exercise intensity exceeds 65% of maximal oxygen uptake carbohydrate energy sources predominate. However, relative to the meager 4–5 g blood glucose pool size in a postabsorptive individual (0.9–1.0 g·L−1 × 5 L blood = 18–20 kcal), carbohydrate (CHO) oxidation rates of 20 kcal·min−1 can be sustained in a healthy and fit person for one hour, if not longer, all the while euglycemia is maintained. While glucose rate of appearance (i.e., production, Ra) from splanchnic sources in a postabsorptive person can rise 2–3 fold during exercise, working muscle and adipose tissue glucose uptake must be restricted while other energy substrates such as glycogen, lactate, and fatty acids are mobilized and utilized. If not for the use of alternative energy substrates hypoglycemia would occur in less than a minute during hard exercise because blood glucose disposal rate (Rd) could easily exceed glucose production (Ra) from hepatic glycogenolysis and gluconeogenesis. The goal of this paper is to present and discuss the integration of physiological, neuroendocrine, circulatory, and biochemical mechanisms necessary for maintenance of euglycemia during sustained hard physical exercise.