bluuebunny/crossref_metadata_2025 huggingface dataset:
The Required Amount of Ventilation Air for the Classroom and the Possibility of Air Infiltration through the Windows
Authors: Lis Piotr , Lis Anna
Published: November 2021

The majority of education buildings in Poland are equipped with natural (gravity) ventilation, where the air inflow depends on the level of window airtightness. A complete statistical urban population of 50 school buildings in Czestochowa have been examined. The main issue to be clarified is the answer to the following questions: Is it theoretically possible to supply enough air to meet the ventilation requirements with gravity ventilation? What is the airtightness of the windows at which it will be possible? The average technical conditions of windows in the analysed buildings were bad. However, only in the case in which high external air leakage coefficient a = 7.0 m3/(h m daPa2/3) (q100KL = 32.4912 m3/(h m) is the amount of air passing through the leaks similar to the quantitative ventilation requirements for classrooms. The quantity of air flowing from the outside through modernized windows that meet the technical requirements (a = 0.6 to 1.0 m3/(m h daPa2/3)) covers on average only about 12% and about 21% of the ventilation needs. Without installing additional vents in the rooms, or better yet, installing mechanical ventilation with heat recovery, meeting the ventilation norm requirements will be impossible.
Thermochemical Recuperation to Enable Efficient Ammonia-Diesel Dual-Fuel Combustion in a Compression Ignition Engine
Authors: Kane Seamus P. , Northrop William F.
Published: November 2021

A thermochemical recuperation (TCR) reactor was developed and experimentally evaluated with the objective to improve dual-fuel diesel–ammonia compression ignition engines. The novel system simultaneously decomposed ammonia into a hydrogen-containing mixture to allow high diesel fuel replacement ratios and oxidized unburned ammonia emissions in the exhaust, overcoming two key shortcomings of ammonia combustion in engines from the previous literature. In the experimental work, a multi-cylinder compression ignition engine was operated in dual-fuel mode using intake-fumigated ammonia and hydrogen mixtures as the secondary fuel. A full-scale catalytic TCR reactor was constructed and generated the fuel used in the engine experiments. The results show that up to 55% of the total fuel energy was provided by ammonia on a lower heating value basis. Overall engine brake thermal efficiency increased for modes with a high exhaust temperature where ammonia decomposition conversion in the TCR reactor was high but decreased for all other modes due to poor combustion efficiency. Hydrocarbon and soot emissions were shown to increase with the replacement ratio for all modes due to lower combustion temperatures and in-cylinder oxidation processes in the late part of heat release. Engine-out oxides of nitrogen (NOx) emissions decreased with increasing diesel replacement levels for all engine modes. A higher concentration of unburned ammonia was measured in the exhaust with increasing replacement ratios. This unburned ammonia predominantly oxidized to NOx species over the oxidation catalyst used within the TCR reactor. Ammonia substitution thus increased post-TCR reactor ammonia and NOx emissions in this work. The results show, however, that engine-out NH3-to-NOx ratios were suitable for passive selective catalytic reduction, thus demonstrating that both ammonia and NOx from the engine could be readily converted to N2 if the appropriate catalyst were used in the TCR reactor.
Formation and Growth Behavior Analysis of Slagging Rings in Rotary Kiln-Type Hazardous Waste Incineration Systems
Authors: Zhao Jing , Zhang Zirui , Li Bo , Wei Xiaolin
Published: November 2021

Rotary kiln incineration technology has the advantages of strong material adaptability and a simple treatment process and has been widely used in hazardous waste treatment. However, the actual incineration process has caused problems such as ring formation in the treatment system due to the lack of research on the slagging mechanisms. In this paper, slagging phenomena occurring in the second half of the rotary kiln, the exit flue of the secondary combustion chamber, and the wall of the quench tower are analyzed and discussed in detail through characterization methods. The results indicate that the adhesion of low-melting alkali metal salts on the refractory surface in the second half of the rotary kiln is the key factor in forming the initial slagging layer. In the growth process of the slagging ring, the formed liquid phase can bond incineration residues of different sizes together and form a dense embryo body through liquid phase sintering. The deposition and solidification of molten/semi-molten fly ashes cause slagging formation in the exit flue of the secondary combustion chamber. The slagging phenomenon occurring in the inner wall of the quench tower belongs to the “crystalline-coalesce-hardening” process of the inorganic salts precipitating out of the high-salt wastewater.
Numerical Analysis of the Convective Heat Transfer Coefficient Enhancement of a Pyro-Breaker Utilized in Superconducting Fusion Facilities
Authors: He Jun , Wang Ke , Li Jiangang
Published: November 2021

The conductive components of the pyro-breaker in the quench protection system (QPS) have high current density, a large number of electrical contacts and high thermal flux. The water system needs to meet the requirements of cooling and arc extinguishing at the same time. In a previous study, the bottleneck of the steady-state capacity appeared in the barrel conductor of the commutation section, which has a cylindrical cavity. The thermal stability of the commutation section at 100 kA level was simulated in ANSYS/Workbench. The results indicate a certain level of enhancement of the convective heat transfer coefficient of the cavity is required to reach the current capacity. However, the fluid flow inside the cavity is very complex, and the convective heat transfer coefficient is difficult to calculate. In this paper, Computational fluid dynamics (CFD) is applied to the optimization of the cooling water system of the pyro-breaker. By studying the enhancement method of convective heat transfer, optimization of the structure and processing method of the water channel are proposed. The convective heat transfer coefficients of the cylindrical cavity in these optimizations were calculated in CFX. A set of optimizations of the cavity, which can meet the requirements of China Fusion Engineering Test Reactor (CFETR), were obtained and verified by experiments.
Misalignment-Tolerant Series Hybrid with Active Adjustable Constant Current and Constant Voltage Output Wireless Charging System
Authors: Gong Zhao-Wei , Li Jin-Gang , Tong Xiang-Qian
Published: November 2021

This paper presents a series hybrid wireless charging system with an active adjustable circuitry offering constant current and constant voltage output characteristics. The series hybrid system consists of the inductor–capacitor–capacitor (LCC) and series-series (SS) networks are used for improving charging pad misalignment tolerance. An active switch is employed to provide an adjustable CC and CV output for different battery charging stages. To demonstrate the performance of the proposed method, a 310 W prototype was built. A systematic optimization in the parameter of the proposed topology to achieve relative constant output was analyzed within a certain range of the designed operating region. The experimental results indicate that the output current fluctuation is less than 5% with load variations, and the output voltage fluctuation is less than 5% with load varying from 19 to 70 Ω, as the pick-up pads misaligned within 50% of the pad outer diameter.
Expression Patterns and Regulation of Non-Coding RNAs during Synthesis of Cellulose in Eucalyptus grandis Hill
Authors: Zhan Ni , Wang Zhen , Xie Yaojian , Shang Xiuhua , Liu Guo , Wu Zhihua
Published: November 2021

Cellulose, an essential structural component in the plant cell wall and a renewable biomass resource, plays a significant role in nature. Eucalyptus’s excellent timber tree species (including Eucalyptus grandis Hill) provide many raw materials for the paper and wood industries. The synthesis of cellulose is a very complex process involving multiple genes and regulated by various biological networks. However, research on regulating associated genes and non-coding RNAs during cellulose synthesis in E. grandis remains lacking. In this study, the wood anatomical characteristics and chemical indexes of E. grandis were analyzed by taking three different parts (diameter at breast height (DBH), middle and upper part of the trunk) from the main stem of E. grandis as raw materials. The role of non-coding RNAs (Long non-coding RNA, lncRNA; Micro RNA, miRNA; Circle RNA, circRNA) on regulating candidate genes was presented, and the network map of ceRNA (Competing endogenous RNA) regulation during wood cellulose biosynthesis of E. grandis was constructed. The transcriptome sequencing of nine samples obtained from the trunk of the immature xylem in E. grandis at DBH, middle and upper parts had a 95.81 G clean reading, 57,480 transcripts, 7365 lncRNAs, and 5180 circRNAs. Each sample had 172–306 known miRNAs and 1644–3508 new miRNAs. A total of 190 DE-lncRNAs (Differentially expressed long non-coding RNAs), 174 DE-miRNAs (Differentially expressed micro RNAs), and 270 DE-circRNAs (Differentially expressed circle RNAs) were obtained by comparing transcript expression levels. Four lncRNAs and nine miRNAs were screened out, and the ceRNA regulatory network was constructed. LncRNA1 and lncRNA4 regulated the genes responsible for cellulose synthesis in E. grandis, which were overexpressed in 84K (Populus Alba × Populus glandulosa) poplar. The cellulose and lignin content in lncRNA4-oe were significantly higher than wild type 84K poplar and lncRNA1-oe. The average plant height, middle and basal part of the stem diameter in lncRNA4-oe were significantly higher than the wild type. However, there was no significant difference between the growth of lncRNA1-oe and the wild type. Further studies are warranted to explore the molecular regulatory mechanism of cellulose biosynthesis in Eucalyptus species.
Climatic Aridity Shapes Post-Fire Interactions between Ceanothus spp. and Douglas-Fir (Pseudotsuga menziesii) across the Klamath Mountains
Authors: Cinoğlu Damla , Epstein Howard E. , Tepley Alan J. , Anderson-Teixeira Kristina J. , Thompson Jonathan R. , Perakis Steven S.
Published: November 2021

Climate change is leading to increased drought intensity and fire frequency, creating early-successional landscapes with novel disturbance–recovery dynamics. In the Klamath Mountains of northwestern California and southwestern Oregon, early-successional interactions between nitrogen (N)-fixing shrubs (Ceanothus spp.) and long-lived conifers (Douglas-fir) are especially important determinants of forest development. We sampled post-fire vegetation and soil biogeochemistry in 57 plots along gradients of time since fire (7–28 years) and climatic water deficit (aridity). We found that Ceanothus biomass increased, and Douglas-fir biomass decreased with increasing aridity. High aridity and Ceanothus biomass interacted with lower soil C:N more than either factor alone. Ceanothus biomass was initially high after fire and declined with time, suggesting a large initial pulse of N-fixation that could enhance N availability for establishing Douglas-fir. We conclude that future increases in aridity and wildfire frequency will likely limit post-fire Douglas-fir establishment, though Ceanothus may ameliorate some of these impacts through benefits to microclimate and soils. Results from this study contribute to our understanding of the effects of climate change and wildfires on interspecific interactions and forest dynamics. Management seeking to accelerate forest recovery after high-severity fire should emphasize early-successional conifer establishment while maintaining N-fixing shrubs to enhance soil fertility.
Forest Structure, Diversity and Dynamics in Terra Firme and Igapó Gallery Forests in the Colombian Orinoco Basin
Authors: González-Abella Juan S. , Aldana Ana M. , Correa Diego F. , Casas Luisa Fernanda , Stevenson Pablo R.
Published: November 2021

Studies of dynamics and biomass accumulation are essential for understanding forest functioning and productivity. They are also valuable when planning strategies for the conservation and sustainable use of natural resources. This study was conducted in five one-hectare plots of gallery forest in the Orinoco basin (three in terra firme and two in igapó floodplains), located in the Tomogrande Reserve, Santa Rosalía municipality, Vichada, Colombia. The differences between these forest types were determined and quantified considering diversity, floristic composition, aboveground biomass, forest dynamics and the influence of soils’ physicochemical composition. Terra firme forests were more diverse (Fisher’s α = 30.4) and had higher mortality and recruitment rates than igapó forests but there were no significant differences in stem turnover rates between forest types. Relative tree growth rate was also higher in terra firme than in igapó, but the opposite was found for biomass change (1.47 t/ha/year in igapó), resulting in an average carbon accumulation rate of 0.33 t/ha/year in terra firme and 0.66 t/ha/year in igapó. Igapó forest has high carbon accumulation potential given the high wood density of certain dominant species. Igapó soils contained a higher concentration of sand, silt, phosphorus and potassium ions, while terra firme soils had a higher clay content with lower pH. In general, the demographic and growth rates were consistent with those described for Amazonian forests. This study highlights the potential of igapó forest in the Orinoco basin as important carbon sinks, which should be included in the management and conservation strategies for this region.
How Safe to Eat Are Raw Bivalves? Host Pathogenic and Public Health Concern Microbes within Mussels, Oysters, and Clams in Greek Markets
Authors: Lattos Athanasios , Chaligiannis Ilias , Papadopoulos Dimitrios , Giantsis Ioannis A. , Petridou Evanthia I. , Vafeas George , Staikou Alexandra , Michaelidis Basile
Published: November 2021

Raw-bivalves consumption is a wide trend in Mediterranean countries. Despite the unambiguous nutritional value of seafood, raw consumption of bivalves may involve risks that could pose a significant threat to consumers’ health. Their filter-feeding behavior is responsible for the potential hosting of a wide variety of microorganisms, either pathogenic for the bivalves or public health threats. Under this prism, the current study was conducted in an effort to evaluate the risk of eating raw bivalves originating from the two biggest seafood markets in Thessaloniki, the largest production area of bivalves in Greece. Both microbiological and molecular methodologies were applied in order to assess the presence of various harmful microbes, including noroviruses, Bonamia, Marteilia, Esherichia coli, Salmonella, and Vibrio. Results indicated the presence of several Vibrio strains in the analyzed samples, of which the halophilic Vibrio harveyi was verified by 16S rRNA sequencing; other than this, no enteropathogenic Vibrio spp. was detected. Furthermore, although Esherichia coli was detected in several samples, it was mostly below the European Union (EU) legislation thresholds. Interestingly, the non-target Photobacterium damselae was also detected, which is associated with both wound infections in human and aquatic animals. Regarding host pathogenic microorganisms, apart from Vibrio harveyi, the protozoan parasite Marteilia refrigens was identified in oysters, highlighting the continuous infection of this bivalve in Greece. In conclusion, bivalves can be generally characterized as a safe-to-eat raw food, hosting more bivalve pathogenic microbes than those of public health concern.
Homemade Kefir Consumption Improves Skin Condition—A Study Conducted in Healthy and Atopic Volunteers
Authors: Alves Emília , Gregório João , Baby André Rolim , Rijo Patrícia , Rodrigues Luis M. , Rosado Catarina
Published: November 2021

Diet has a fundamental role in the homeostasis of bodily functions, including the skin, which, as an essential protective barrier, plays a crucial role in this balance. The skin and intestine appear to share a series of indirect metabolic pathways, in a dual relationship known as the “gut-skin axis”. Hence, the gut-skin axis might be receptive to modulation via dietary modification, where probiotics can be included, thus representing a potential therapeutic target in inflammatory skin diseases, such as atopic dermatitis (AD), in order to control and/or ameliorate symptoms. Kefir is one of the most ancient fermented foods, with probiotic characteristics that have been associated with a wide variety of health-promoting benefits, and it presents a microbiological diversity that makes its application as a probiotic in the gut-skin relationship of the utmost interest. However, the impact of a diet containing kefir on skin health has yet to be reported in scientific literature. This study aimed to assess the impact of the intake of homemade kefir in the skin of healthy and atopic volunteers. The intervention resulted in a boost on barrier function in both skin types verified only in the respective kefir intake groups. An improvement in the degree of severity of AD was also confirmed for the kefir intake group. Atopic individuals may benefit from kefir intake, especially in regard to their skin hydration. Finally, the effects observed on skin barrier function in this study probably culminate from the effects of all the ingredients in kefir, including the complex microbiota, its metabolites and macro- and micronutrients resulting from the fermentation. This work opens the way for more advanced research on the impact of the probiotic kefir on cutaneous health, further clarifying its mechanism of action namely via gut-skin axis.
Optimization of Microwave-Assisted Green Method for Enhanced Solubilization of Water-Soluble Curcuminoids Prepared Using Steviol Glycosides
Authors: Ko Jin-A , Ryu Young-Bae , Lee Woo-Song , Ameer Kashif , Kim Young-Min
Published: November 2021

In this study, the optimization and modeling of microwave-assisted extraction (MAE) of water-soluble curcuminoids prepared using novel steviol glycosides (SGs) was carried out using four independent process variables at varying levels—X1: microwave power (50–200 W), X2: stevioside concentration (50–200 mg/mL), X3: curcumin concentration (20–200 mg/mL), and X4: time (1–10 min)—in response surface methodology configuration. Moreover, the effects of stevioside, as the most cost-effective natural solubilizer, were also evaluated. The water solubility of curcuminoids increased from 11 to 1320 mg/L with the addition of stevioside as a natural solubilizer. Moreover, microwave heating synergistically with stevioside addition significantly (p < 0.05) increased the solubility up to 5400 mg/L. Based on the results, the optimum conditions providing the maximum solubilization of 16,700 mg/L were 189 W microwave power, 195 g/L stevioside concentration, 183 g/L curcuminoid concentration, and 9 min of incubation time. Moreover, MAE of curcuminoids using SGs might render a significant advantage for its wide-scale application to solubilizing the multitude of insoluble functional flavonoids in fruits, plants, and food materials.
Accelerated Solvent Extraction of Antioxidant Compounds from Gardeniae Fructus and Its Acetylcholinesterase Inhibitory and PC12 Cell Protective Activities
Authors: Fan Yiling , Li Xueying , Ding Lan , Zhou Weiying , Xu Guangzhi , Wang Yan , Zhang Youzuo , Ni Qinxue
Published: November 2021

Gardeniae fructus is a common neuroprotective medicinal food in China, however the extraction efficiency and mixture activities are rarely mentioned. In this study, accelerated solvent extraction (ASE) parameters were optimized by a response surface methodology to extract antioxidants from Gardeniae fructus. Neuroprotective activity was evaluated using H2O2 and amyloid-β25–35 peptide-treated PC12 cells. By comparing with three other extract methods (i.e., heated refluxing extraction (HRE), ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE)), it was found that the yield (35.10%), total iridoids (27.69%), total flavonoid (6.12%) content, antioxidant activities (IC50 on DPPH, 164.46 µg/mL; FRAP value 4703.54 μmol/L), and acetylcholinesterase inhibitory ability (IC50 92.58 µg/mL) of ASE extract under the optimal condition (150 °C temperature, 10 min static time, 60% ethanol, 2 extract cycles) were significantly higher than other extract methods. The strongest ability to protect PC12 cells from damage was also present in ASE extract, as evidenced by decreasing lactate dehydrogenase and malondialdehyde levels, elevating superoxide dismutase and glutathioneperoxidase activities. Compositional analysis indicated that the extremely high crocetin level in ASE extract (1.30 μg/mg) may offer great potential. Our results indicated that ASE is a proper extraction method that could offer great potential for finding the neuroprotective ability of Gardeniae fructus for the treatment of AD.
Influence of Different Types of Polysaccharide-Based Coatings on the Storage Stability of Fresh-Cut Kiwi Fruit: Assessing the Physicochemical, Antioxidant and Phytochemical Properties
Authors: Guroo Ishrat , Gull Amir , Wani Sajad Mohd , Wani Sajad Ahmad , Al-Huqail Asma A. , Alhaji Jwaher Haji
Published: November 2021

The present study focuses on studying the influence of various edible biopolymer coatings at several concentrations on physicochemical, antioxidant and lipid peroxidation activity levels of biopolymer-coated fresh-cut kiwi slices stored at room temperature (relative humidity: 90%). Kiwi slices were coated by dipping in xanthan gum (0.1, 0.2, 0.3% w/v), alginate (1, 2, 3% w/v) and chitosan (0.25, 0.50, 0.75% w/v) solutions for 2 min. Kiwi fruit slices without any treatment were designated as the control. Compared to the control, all coated samples retained higher ascorbic acid, titratable acidity, total phenolic component and antioxidant capacity levels. However, xanthan-gum-coated slices retained significantly higher amounts of total phenolics in comparison to alginate- and chitosan-coated slices (p ≤ 0.05). HPLC analysis showed the presence of neochlorogenic acid, chlorogenic acid, ellagic acid and epicatechin. The results suggest that the xanthan gum can be utilized to enhance the shelf life of fresh-cut kiwi slices without compromising quality.
Technique to Solve Linear Fractional Differential Equations Using B-Polynomials Bases
Authors: Bhatti Muhammad I. , Rahman Md. Habibur
Published: November 2021

A multidimensional, modified, fractional-order B-polys technique was implemented for finding solutions of linear fractional-order partial differential equations. To calculate the results of the linear Fractional Partial Differential Equations (FPDE), the sum of the product of fractional B-polys and the coefficients was employed. Moreover, minimization of error in the coefficients was found by employing the Galerkin method. Before the Galerkin method was applied, the linear FPDE was transformed into an operational matrix equation that was inverted to provide the values of the unknown coefficients in the approximate solution. A valid multidimensional solution was determined when an appropriate number of basis sets and fractional-order of B-polys were chosen. In addition, initial conditions were applied to the operational matrix to seek proper solutions in multidimensions. The technique was applied to four examples of linear FPDEs and the agreements between exact and approximate solutions were found to be excellent. The current technique can be expanded to find multidimensional fractional partial differential equations in other areas, such as physics and engineering fields.
Quantum Gravity Phenomenology Induced in the Propagation of UHECR, a Kinematical Solution in Finsler and Generalized Finsler Spacetime
Authors: Torri Marco Danilo Claudio
Published: November 2021

It is well-known that the universe is opaque to the propagation of Ultra-High-Energy Cosmic Rays (UHECRs) since these particles dissipate energy during their propagation interacting with the background fields present in the universe, mainly with the Cosmic Microwave Background (CMB) in the so-called GZK cut-off phenomenon. Some experimental evidence seems to hint at the possibility of a dilation of the GZK predicted opacity sphere. It is well-known that kinematical perturbations caused by supposed quantum gravity (QG) effects can modify the foreseen GZK opacity horizon. The introduction of Lorentz Invariance Violation can indeed reduce, and in some cases making negligible, the CMB-UHECRs interaction probability. In this work, we explore the effects induced by modified kinematics in the UHECR lightest component phenomenology from the QG perspective. We explore the possibility of a geometrical description of the massive fermions interaction with the supposed quantum structure of spacetime in order to introduce a Lorentz covariance modification. The kinematics are amended, modifying the dispersion relations of free particles in the context of a covariance-preserving framework. This spacetime description requires a more general geometry than the usual Riemannian one, indicating, for instance, the Finsler construction and the related generalized Finsler spacetime as ideal candidates. Finally we investigate the correlation between the magnitude of Lorentz covariance modification and the attenuation length of the photopion production process related to the GZK cut-off, demonstrating that the predicted opacity horizon can be dilated even in the context of a theory that does not require any privileged reference frame.
Phytochemical-Based Nano-Pharmacotherapeutics for Management of Burn Wound Healing
Authors: Qadir Abdul , Jahan Samreen , Aqil Mohd , Warsi Musarrat Husain , Alhakamy Nabil A. , Alfaleh Mohamed A. , Khan Nausheen , Ali Athar
Published: November 2021

Medicinal plants have been used since ancient times for their various therapeutic activities and are safer compared to modern medicines, especially when properly identifying and preparing them and choosing an adequate dose administration. The phytochemical compounds present in plants are progressively yielding evidence in modern drug delivery systems by treating various diseases like cancers, coronary heart disease, diabetes, high blood pressure, inflammation, microbial, viral and parasitic infections, psychotic diseases, spasmodic conditions, ulcers, etc. The phytochemical requires a rational approach to deliver the compounds to enhance the efficacy and to improve patients’ compatibility. Nanotechnology is emerging as one of the most promising strategies in disease control. Nano-formulations could target certain parts of the body and control drug release. Different studies report that phytochemical-loaded nano-formulations have been tested successfully both in vitro and in vivo for healing of skin wounds. The use of nano systems as drug carriers may reduce the toxicity and enhance the bioavailability of the incorporated drug. In this review, we focus on various nano-phytomedicines that have been used in treating skin burn wounds, and how both nanotechnology and phytochemicals are effective for treating skin burns.
Constructing Cellulose Diacetate Aerogels with Pearl-Necklace-like Skeleton Networking Structure
Authors: Xiong Shixian , Hu Yangbiao , Zhang Sizhao , Xiao Yunyun , Li Zhengquan
Published: November 2021

Cellulose and its derivative aerogels have attracted much attention due to their renewable and biodegradable properties. However, the significant shrinkage in the supercritical drying process causes the relatively high thermal conductivity and low mechanical property of cellulose and its derivatives aerogels. Considering the pearl-necklace-like skeleton network of silica aerogels, which can improve thermal insulation property and mechanical property. Herein, we propose a new strategy for fabricating cellulose diacetate aerogels (CDAAs) with pearl-necklace-like skeletons by using tert-butanol (TBA) as exchange solvent after experiencing the freezing-drying course. CDAAs obtained have the low density of 0.09 g cm−3, the nanopore size in the range of 10–40 nm, the low thermal conductivity of 0.024 W m−1 K−1 at ambient conditions, and the excellent mechanical properties (0.18 MPa at 3% strain, 0.38 MPa at 5% strain). Ultimately, CDAAs with moderate mechanical property paralleled to cellulose-derived aerogels obtained from supercritical drying process are produced, only simultaneously owning the radial shrinkage of 6.2%. The facile method for fabricating CDAAs could provide a new reference for constructing cellulose/cellulose-derived aerogels and other biomass aerogels.
Genealogy: The Tree Where History Meets Genetics
Authors: Gomes Cláudia , Palomo-Díez Sara , López-Parra Ana María , Arroyo-Pardo Eduardo
Published: November 2021

Although biological relationships are a universal reality for all human beings, the concepts of “family” and “family bond” depend on both the geographic region and the historical moment to which they refer. However, the concept of “family” can be determinant in a large variety of societies, since it can influence the lines of succession, inheritances and social relationships, as well as where and with whom an individual is buried. The relation between a deceased person and other members of a community, other individuals of the same necropolis, or even with those who are buried in the same tomb can be analysed from the genetic point of view, considering different perspectives: archaeological, historical, and forensic. In the present work, the concepts of “family” and “kinship” are discussed, explaining the relevance of genetic analysis, such as nuclear and lineage markers, and their contribution to genealogical research, for example in the heritage of surnames and Y-chromosome, as well as those cases where some discrepancies with historical record are detected, such as cases of adoption. Finally, we explain how genetic genealogical analyses can help to solve some cold cases, through the analysis of biologically related relatives.
Integration and Visualization of Regulatory Elements and Variations of the EPAS1 Gene in Human
Authors: Kristan Aleša , Debeljak Nataša , Kunej Tanja
Published: November 2021

Endothelial PAS domain-containing protein 1 (EPAS1), also HIF2α, is an alpha subunit of hypoxia-inducible transcription factor (HIF), which mediates cellular and systemic response to hypoxia. EPAS1 has an important role in the transcription of many hypoxia-responsive genes, however, it has been less researched than HIF1α. The aim of this study was to integrate an increasing number of data on EPAS1 into a map of diverse OMICs elements. Publications, databases, and bioinformatics tools were examined, including Ensembl, MethPrimer, STRING, miRTarBase, COSMIC, and LOVD. The EPAS1 expression, stability, and activity are tightly regulated on several OMICs levels to maintain complex oxygen homeostasis. In the integrative EPAS1 map we included: 31 promoter-binding proteins, 13 interacting miRNAs and one lncRNA, and 16 post-translational modifications regulating EPAS1 protein abundance. EPAS1 has been associated with various cancer types and other diseases. The development of neuroendocrine tumors and erythrocytosis was shown to be associated with 11 somatic and 20 germline variants. The integrative map also includes 12 EPAS1 target genes and 27 interacting proteins. The study introduced the first integrative map of diverse genomics, transcriptomics, proteomics, regulomics, and interactomics data associated with EPAS1, to enable a better understanding of EPAS1 activity and regulation and support future research.
Editorial for the Genetics of Alzheimer’s Disease Special Issue: October 2021
Authors: Ibanez Laura , Miller Justin B.
Published: November 2021

Alzheimer’s disease is a complex and multifactorial condition regulated by both genetics and lifestyle, which ultimately results in the accumulation of β-amyloid (Aβ) and tau proteins in the brain, loss of gray matter, and neuronal death [...]
Somatic Embryogenesis and Indirect In Vitro Plant Regeneration in Amorphophallus konjac K. Koch by One-Step Seedling Formation
Authors: Li Dandan , Mohammadi Mohammad Aqa , Qin Yuan , Zhang Zongshen
Published: November 2021

Konjac (Amorphophallus konjac K. Koch) is a well-known tuberous vegetable belonging to the important medicinal family Araceae, and the plant grows from an underground tuber. Here, we used a “one-step seedling regeneration” tissue culture system to improve the plantlet regeneration efficiency of konjac using young leaves as an explant source. In the current study, we used several sterilization methods for tuber sterilization. Moreover, various plant growth regulator combinations were applied to achieve efficient somatic embryogenesis and plantlet regeneration. Our results showed that the optimal tuber sterilization was method C (75% alcohol for 15 s + 0.1% HgCl2 for 15 min + washing by double-sterilized water three times). Three types of embryogenic calli were induced on full-strength Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L of 6-benzylaminopurine (6-BA), 0.5 mg/L of naphthaleneacetic acid (NAA), 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D), and 30 g/L of sucrose. Of the three types of embryogenic calli, only type Ⅲ further regenerated plantlets, with a callus induction rate of 55.73% and a seedling induction rate of 92.73%. This suggests that the addition of the above hormones gives the optimal callus induction. The proliferation rate achieved was 38% on the MS basal medium containing 1.0 mg/L of 6-BA, 1.0 mg/L of indolebutyric acid (IBA), 0.2 mg/L of kinetin (KT), and 50 g/L of sucrose. The one-step seedling formation achieved in MS medium contained 2.0 mg/L of 6-BA, 0.5 mg/L of NAA, 0.1 mg/L of gibberellic acid (GA3), and 30 g/L of sucrose, and the number of regenerated shoots per explants was 6 ± 2. Therefore, we establish a one-step seedling regeneration system through indirect plant regeneration, which shortens the time for konjac in vitro regeneration, significantly increased the micropropagation efficiency, and decreased the cost of the konjac tissue culture.
Using the Behavioural Regulation in an Exercise Questionnaire (BREQ–2) in Central and Eastern Europe: Evidence of Reliability, Sociocultural Background, and the Effect on Sports Activity
Authors: Kovács Klára , Kovács Karolina Eszter
Published: November 2021

The factors influencing sports motivation create a complex system, integrating internal drivers, such as the love of sport and the need for competence, and external segments, such as the environment, institutions, or the media. In our research, we examined the sports motivation of students studying in higher educational institutions in five countries (Hungary, Romania, Serbia, Slovakia, and Ukraine) using the Behavioral Regulation in Exercise Questionnaire (BREQ–2). This research aims to explore the socioeconomic and institutional factors influencing students’ sports motivation and the effect of sociocultural, demographical, motivational, and societal factors on the frequency of participating in sports. Based on factor analysis, instead of the original five factors, three factors could be detected in the sample: intrinsic and identified motivation, introjected motivation, extrinsic motivation, and amotivation. Based on the results, higher social status facilitates intrinsic motivation, while lower socioeconomic status facilitates extrinsic motivation and amotivation. The strongest effect is exerted by individual variables, of which intrinsic and identified motivation support regular physical activity as a significant factor among students in all countries. This can also be seen in the frequency of sporting activities, as the highest frequency of sports activity was detected among people with high intrinsic motivation.
EnvSLAM: Combining SLAM Systems and Neural Networks to Improve the Environment Fusion in AR Applications
Authors: Marchesi Giulia , Eichhorn Christian , Plecher David A. , Itoh Yuta , Klinker Gudrun
Published: November 2021

Augmented Reality (AR) has increasingly benefited from the use of Simultaneous Localization and Mapping (SLAM) systems. This technology has enabled developers to create AR markerless applications, but lack semantic understanding of their environment. The inclusion of this information would empower AR applications to better react to the surroundings more realistically. To gain semantic knowledge, in recent years, focus has shifted toward fusing SLAM systems with neural networks, giving birth to the field of Semantic SLAM. Building on existing research, this paper aimed to create a SLAM system that generates a 3D map using ORB-SLAM2 and enriches it with semantic knowledge originated from the Fast-SCNN network. The key novelty of our approach is a new method for improving the predictions of neural networks, employed to balance the loss of accuracy introduced by efficient real-time models. Exploiting sensor information provided by a smartphone, GPS coordinates are utilized to query the OpenStreetMap database. The returned information is used to understand which classes are currently absent in the environment, so that they can be removed from the network’s prediction with the goal of improving its accuracy. We achieved 87.40% Pixel Accuracy with Fast-SCNN on our custom version of COCO-Stuff and showed an improvement by involving GPS data for our self-made smartphone dataset resulting in 90.24% Pixel Accuracy. Having in mind the use on smartphones, the implementation aimed to find a trade-off between accuracy and efficiency, making the system achieve an unprecedented speed. To this end, the system was carefully designed and a strong focus on lightweight neural networks is also fundamental. This enabled the creation of an above real-time Semantic SLAM system that we called EnvSLAM (Environment SLAM). Our extensive evaluation reveals the efficiency of the system features and the operability in above real-time (48.1 frames per second with an input image resolution of 640 × 360 pixels). Moreover, the GPS integration indicates an effective improvement of the network’s prediction accuracy.
Ultrasensitive Detection of Bacillus anthracis by Real-Time PCR Targeting a Polymorphism in Multi-Copy 16S rRNA Genes and Their Transcripts
Authors: Braun Peter , Nguyen Martin Duy-Thanh , Walter Mathias C. , Grass Gregor
Published: November 2021

The anthrax pathogen Bacillus anthracis poses a significant threat to human health. Identification of B. anthracis is challenging because of the bacterium’s close genetic relationship to other Bacillus cereus group species. Thus, molecular detection is founded on species-specific PCR targeting single-copy genes. Here, we validated a previously recognized multi-copy target, a species-specific single nucleotide polymorphism (SNP) present in 2–5 copies in every B. anthracis genome analyzed. For this, a hydrolysis probe-based real-time PCR assay was developed and rigorously tested. The assay was specific as only B. anthracis DNA yielded positive results, was linear over 9 log10 units, and was sensitive with a limit of detection (LoD) of 2.9 copies/reaction. Though not exhibiting a lower LoD than established single-copy PCR targets (dhp61 or PL3), the higher copy number of the B. anthracis–specific 16S rRNA gene alleles afforded ≤2 unit lower threshold (Ct) values. To push the detection limit even further, the assay was adapted for reverse transcription PCR on 16S rRNA transcripts. This RT-PCR assay was also linear over 9 log10 units and was sensitive with an LoD of 6.3 copies/reaction. In a dilution series of experiments, the 16S RT-PCR assay achieved a thousand-fold higher sensitivity than the DNA-targeting assays. For molecular diagnostics, we recommend a real-time RT-PCR assay variant in which both DNA and RNA serve as templates (thus, no requirement for DNase treatment). This can at least provide results equaling the DNA-based implementation if no RNA is present but is superior even at the lowest residual rRNA concentrations.
SARS-CoV-2, Cardiovascular Diseases, and Noncoding RNAs: A Connected Triad
Authors: Natarelli Lucia , Virgili Fabio , Weber Christian
Published: November 2021

Coronavirus Disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is characterized by important respiratory impairments frequently associated with severe cardiovascular damages. Moreover, patients with pre-existing comorbidity for cardiovascular diseases (CVD) often present a dramatic increase in inflammatory cytokines release, which increases the severity and adverse outcomes of the infection and, finally, mortality risk. Despite this evident association at the clinical level, the mechanisms linking CVD and COVID-19 are still blurry and unresolved. Noncoding RNAs (ncRNAs) are functional RNA molecules transcribed from DNA but usually not translated into proteins. They play an important role in the regulation of gene expression, either in relatively stable conditions or as a response to different stimuli, including viral infection, and are therefore considered a possible important target in the design of specific drugs. In this review, we introduce known associations and interactions between COVID-19 and CVD, discussing the role of ncRNAs within SARS-CoV-2 infection from the perspective of the development of efficient pharmacological tools to treat COVID-19 patients and taking into account the equally dramatic associated consequences, such as those affecting the cardiovascular system.
Plant-Growth-Promoting Rhizobacteria Emerging as an Effective Bioinoculant to Improve the Growth, Production, and Stress Tolerance of Vegetable Crops
Authors: Kumar Manoj , Giri Ved Prakash , Pandey Shipra , Gupta Anmol , Patel Manish Kumar , Bajpai Atal Bihari , Jenkins Sasha , Siddique Kadambot H. M.
Published: November 2021

Vegetable cultivation is a promising economic activity, and vegetable consumption is important for human health due to the high nutritional content of vegetables. Vegetables are rich in vitamins, minerals, dietary fiber, and several phytochemical compounds. However, the production of vegetables is insufficient to meet the demand of the ever-increasing population. Plant-growth-promoting rhizobacteria (PGPR) facilitate the growth and production of vegetable crops by acquiring nutrients, producing phytohormones, and protecting them from various detrimental effects. In this review, we highlight well-developed and cutting-edge findings focusing on the role of a PGPR-based bioinoculant formulation in enhancing vegetable crop production. We also discuss the role of PGPR in promoting vegetable crop growth and resisting the adverse effects arising from various abiotic (drought, salinity, heat, heavy metals) and biotic (fungi, bacteria, nematodes, and insect pests) stresses.
Mold, Mycotoxins and a Dysregulated Immune System: A Combination of Concern?
Authors: Kraft Stephanie , Buchenauer Lisa , Polte Tobias
Published: November 2021

Fungi represent one of the most diverse and abundant eukaryotes on earth. The interplay between mold exposure and the host immune system is still not fully elucidated. Literature research focusing on up-to-date publications is providing a heterogenous picture of evidence and opinions regarding the role of mold and mycotoxins in the development of immune diseases. While the induction of allergic immune responses by molds is generally acknowledged, other direct health effects like the toxic mold syndrome are controversially discussed. However, recent observations indicate a particular importance of mold/mycotoxin exposure in individuals with pre-existing dysregulation of the immune system, due to exacerbation of underlying pathophysiology including allergic and non-allergic chronic inflammatory diseases, autoimmune disorders, and even human immunodeficiency virus (HIV) disease progression. In this review, we focus on the impact of mycotoxins regarding their impact on disease progression in pre-existing immune dysregulation. This is complemented by experimental in vivo and in vitro findings to present cellular and molecular modes of action. Furthermore, we discuss hypothetical mechanisms of action, where evidence is missing since much remains to be discovered.
Non-Surgical Periodontal Therapy Could Improve the Periodontal Inflammatory Status in Patients with Periodontitis and Chronic Hepatitis C
Authors: Gheorghe Dorin Nicolae , Popescu Dora Maria , Salan Alex , Boldeanu Mihail Virgil , Ionele Claudiu Marinel , Pitru Allma , Turcu-Stiolica Adina , Camen Adrian , Florescu Cristina , Rogoveanu Ion , Surlin Petra
Published: November 2021

Non-surgical periodontal therapy (NSPT) is the first essential step for the management of any periodontitis patient. This study aims to evaluate the impact of NSPT on pro-inflammatory mediators’ regulation and on clinical parameters in periodontitis patients who suffer from chronic hepatitis C. At baseline, selected patients were clinically evaluated for their periodontal status. A subsequent quantitative assessment of C-reactive protein and pentraxin-3 in samples of gingival fluid was performed by Enzyme-Linked Immunosorbent Assay (ELISA). Afterwards, NSPT was performed. Three months after NSPT, the clinical and ELISA assessments were repeated. The results show an improvement of the clinical parameters in periodontitis patients at the three-month recall. In chronic hepatitis C patients with periodontitis, the gingival fluid levels of pro-inflammatory markers reduced significantly. The targeted markers also expressed significant correlations with the clinical parameters used for the assessment of periodontitis’ severity. The results suggest that, while chronic hepatitis C patients exhibited a more negative periodontal status at baseline as compared to non-hepatitis ones, NSPT is effective in decreasing the local periodontal inflammatory reaction and in proving the periodontal status of this type of patients. Given the limitation of the study, periodontal screening and NSPT should be included in the integrated therapeutical approach of chronic hepatitis C patients, for its impact on the local inflammatory response.
The Clinical Significance of Cerebrospinal Fluid Reticulon 4 (RTN4) Levels in the Differential Diagnosis of Neurodegenerative Diseases
Authors: Kulczyńska-Przybik Agnieszka , Dulewicz Maciej , Słowik Agnieszka , Borawska Renata , Kułakowska Alina , Kochanowicz Jan , Mroczko Barbara
Published: November 2021

Neurodegenerative diseases (NDs) belong to the top global causes of mortality. Diagnostic approaches to improve early diagnosis and differentiation of these diseases are constantly being sought. Therefore, we aimed to assess the cerebrospinal fluid (CSF) concentrations of Reticulon 4 (RTN4) in patients with neurodegenerative diseases and evaluate the potential clinical usefulness of this protein. RTNs are transmembrane proteins mediating neuroanatomical plasticity and functional recovery after central nervous system injury or diseases. According to our best knowledge, this is the first investigation providing the data concerning the dynamic of CSF RTN4 protein levels in patients with different NDs. Methods: Overall, 77 newly diagnosed patients with neurodegenerative diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD), and multiple sclerosis (MS), as well as 21 controls, were enrolled in the study. The CSF concentrations of tested proteins were assessed using immunological assays. Results: We revealed significantly higher CSF RTN4A levels in patients with AD, PD, and MS in comparison to the controls. Moreover, the comparative analysis of RTN4 concentration between different neurodegenerative diseases revealed the highest concentration of RTN4A in AD patients and a statistically significant difference between AD vs. PD, and AD vs. MS groups. The increased CSF level of the protein correlated with Tau, and pTau181 proteins in AD as well as in PD patients. Conclusions: Our study presents a previously not identified clinical utility of RTN4 in the differential diagnosis of neurodegenerative diseases.