bluuebunny/crossref_metadata_2025 huggingface dataset:
Chinese Traffic Police Gesture Recognition Based on Graph Convolutional Network in Natural Scene
Authors: Liu Kang , Zheng Ying , Yang Junyi , Bao Hong , Zeng Haoming
Published: December 2021

For an automated driving system to be robust, it needs to recognize not only fixed signals such as traffic signs and traffic lights, but also gestures used by traffic police. With the aim to achieve this requirement, this paper proposes a new gesture recognition technology based on a graph convolutional network (GCN) according to an analysis of the characteristics of gestures used by Chinese traffic police. To begin, we used a spatial–temporal graph convolutional network (ST-GCN) as a base network while introducing the attention mechanism, which enhanced the effective features of gestures used by traffic police and balanced the information distribution of skeleton joints in the spatial dimension. Next, to solve the problem of the former graph structure only representing the physical structure of the human body, which cannot capture the potential effective features, this paper proposes an adaptive graph structure (AGS) model to explore the hidden feature between traffic police gesture nodes and a temporal attention mechanism (TAS) to extract features in the temporal dimension. In this paper, we established a traffic police gesture dataset, which contained 20,480 videos in total, and an ablation study was carried out to verify the effectiveness of the method we proposed. The experiment results show that the proposed method improves the accuracy of traffic police gesture recognition to a certain degree; the top-1 is 87.72%, and the top-3 is 95.26%. In addition, to validate the method’s generalization ability, we also carried out an experiment on the Kinetics–Skeleton dataset in this paper; the results show that the proposed method is better than some of the existing action-recognition algorithms.
Vibrational Imaging Techniques for the Characterization of Hard Dental Tissues: From Bench-Top to Chair-Side
Authors: Orsini Giovanna , Orilisi Giulia , Notarstefano Valentina , Monterubbianesi Riccardo , Vitiello Flavia , Tosco Vincenzo , Belloni Alessia , Putignano Angelo , Giorgini Elisabetta
Published: December 2021

Currently, various analytical techniques, including scanning electron microscopy, X-Ray diffraction, microcomputed tomography, and energy dispersive X-ray spectroscopy, are available to study the structural or elemental features of hard dental tissues. In contrast to these approaches, Raman Microspectroscopy (RMS) has the great advantage of simultaneously providing, at the same time and on the same sample, a morpho-chemical correlation between the microscopic information from the visual analysis of the sample and its chemical and macromolecular composition. Hence, RMS represents an innovative and non-invasive technique to study both inorganic and organic teeth components in vitro. The aim of this narrative review is to shed new light on the applicative potential of Raman Microspectroscopy in the dental field. Specific Raman markers representative of sound and pathological hard dental tissues will be discussed, and the future diagnostic application of this technique will be outlined. The objective and detailed information provided by this technique in terms of the structure and chemical/macromolecular components of sound and pathological hard dental tissues could be useful for improving knowledge of several dental pathologies. Scientific articles regarding RMS studies of human hard dental tissues were retrieved from the principal databases by following specific inclusion and exclusion criteria.
Statistical Modeling for PM10, PM2.5 and PM1 at Gangneung Affected by Local Meteorological Variables and PM10 and PM2.5 at Beijing for Non- and Dust Periods
Authors: Choi Soo-Min , Choi Hyo
Published: December 2021

Multiple statistical prediction modeling of PM10, PM2.5 and PM1 at Gangneung city, Korea, was performed in association with local meteorological parameters (air temperature, wind speed and relative humidity) and PM10 and PM2.5 concentrations of an upwind site in Beijing, China, in the transport route of Chinese yellow dusts which originated from the Gobi Desert and passed through Beijing to the city from 18 March to 27 March 2015. Before and after the dust periods, the PM10, PM2.5 and PM1 concentrations showed as being very high at 09:00 LST (the morning rush hour) by the increasing emitted pollutants from vehicles and flying dust from the road and their maxima occurred at 20:00 to 22:00 LST (the evening departure time) from the additional pollutants from resident heating boilers. During the dust period, these peak trends were not found due to the persistent accumulation of dust in the city from the Gobi Desert through Beijing, China, as shown in real-time COMS-AI satellite images. Multiple correlation coefficients among PM10, PM2.5 and PM1 at Gangneung were in the range of 0.916 to 0.998. Multiple statistical models were devised to predict each PM concentration, and the significant levels through multi-regression analyses were p < 0.001, showing all the coefficients to be significant. The observed and calculated PM concentrations were compared, and new linear regression models were sequentially suggested to reproduce the original observed PM values with improved correlation coefficients, to some extent.
Genomic Analysis of 18th-Century Kazakh Individuals and Their Oral Microbiome
Authors: White Anna E. , de-Dios Toni , Carrión Pablo , Bonora Gian Luca , Llovera Laia , Cilli Elisabetta , Lizano Esther , Khabdulina Maral K. , Tleugabulov Daniyar T. , Olalde Iñigo , Marquès-Bonet Tomàs , Balloux François , Pettener Davide , van Dorp Lucy , Luiselli Donata , Lalueza-Fox Carles
Published: December 2021

The Asian Central Steppe, consisting of current-day Kazakhstan and Russia, has acted as a highway for major migrations throughout history. Therefore, describing the genetic composition of past populations in Central Asia holds value to understanding human mobility in this pivotal region. In this study, we analyse paleogenomic data generated from five humans from Kuygenzhar, Kazakhstan. These individuals date to the early to mid-18th century, shortly after the Kazakh Khanate was founded, a union of nomadic tribes of Mongol Golden Horde and Turkic origins. Genomic analysis identifies that these individuals are admixed with varying proportions of East Asian ancestry, indicating a recent admixture event from East Asia. The high amounts of DNA from the anaerobic Gram-negative bacteria Tannerella forsythia, a periodontal pathogen, recovered from their teeth suggest they may have suffered from periodontitis disease. Genomic analysis of this bacterium identified recently evolved virulence and glycosylation genes including the presence of antibiotic resistance genes predating the antibiotic era. This study provides an integrated analysis of individuals with a diet mostly based on meat (mainly horse and lamb), milk, and dairy products and their oral microbiome.
Itch in Adult Population with Type 2 Diabetes Mellitus: Clinical Profile, Pathogenesis and Disease-Related Burden in a Cross-Sectional Study
Authors: Stefaniak Aleksandra A. , Krajewski Piotr K. , Bednarska-Chabowska Dorota , Bolanowski Marek , Mazur Grzegorz , Szepietowski Jacek C.
Published: December 2021

Background: Despite growing interest in itch, data regarding itch in type 2 diabetes mellitus (DM2) are still limited, and mostly based on outdated studies. This study aimed to evaluate the clinical characteristics of itch in the adult population with DM2 and explore potential underlying causes. Methods: The study group consisted of 109 adult patients with DM2. Standardized questionnaires were completed in order to assess the itch intensity [Numerical Rating Scale (three days, 24hours) (NRS)] and the Four-item Itch Questionnaire (4IIQ) and to assess the psychological impact of itch [ItchyQoL, Six-Item Stigmatization Scale (6-ISS), Hospital Anxiety and Depression Scale (HADS)]. Skin dryness was evaluated clinically and by non-invasive assessment of epidermis moisturizing. Neuropathy was assessed using the clinical Katzenwadel neuropathy scale. Results: Itch occurred in 35.8% of adult patients with DM2, with NRSmax three days 6.31 ± 2.16 and 8.1 ± 3.5 points in 4IIQ. Itchy patients have had significantly higher FPG levels compared with the non-itchy population (p = 0.01). Patients with itch had a significantly higher possibility of neuropathy compared with non-itchy subjects (p < 0.01). Skin xerosis was significantly more advanced in patients with itch compared to those without (p < 0.01). The mean ItchyQol score was assessed as 41.2 ± 13.4 points, indicating mild life quality impairment and correlated positively with itch intensity. Itchy subjects had significantly higher scores in both anxiety and depression dimensions of HADS (in each p < 0.01). Conclusions: We suggest that the primary cause of itch is prolonged poor diabetes control with altered glucose and insulin levels, subsequently causing skin dryness and neuropathy in long-lasting DM2.
Evaluation of Photovoltaic Energy Saving Potential and Investment Value of Urban Buildings in China Based on GIS Technology
Authors: Zhang Wenjie , Huang Fengcheng , Mao Kai , Lin Changqing , Pan Zhen
Published: December 2021

The environmental and energy crisis has become a problem that can not be ignored in today’s world and improving the proportion of renewable energy utilization is an important way to alleviate the problem. China has begun to vigorously develop rooftop photovoltaic systems, and it is urgent to analyze the photovoltaic potential of the country. In this paper, GIS technology is used to calculate the available area of a PV system on the roof of urban buildings in China. The installed capacity and annual power generation of a PV system on the roof of urban buildings in China are further calculated, and the investment cost analysis of the buildings with a PV system is carried out. The results show that Chinese mainland city rooftop photovoltaic area has reached 3.35 billion m2. If urban roofs are used for photovoltaic power generation in China, the annual photovoltaic power generation capacity will be 672 billion kWh, which is about 61% of the total annual electricity consumption of the whole society in 2020. On the other hand, the initial investment in the construction of an urban photovoltaic system is large, but most of them can realize the return of investment in a short period of 8–10 years. Moreover, in the effective life of the follow-up photovoltaic system, it will bring greater economic benefits, which is more than two times the initial investment. To further promote the process of social carbon peak and carbon neutrality in China, the construction of an urban photovoltaic system is very feasible.
Characterization of Type 1 Angiotensin II Receptor Activation Induced Dual-Specificity MAPK Phosphatase Gene Expression Changes in Rat Vascular Smooth Muscle Cells
Authors: Gém Janka Borbála , Kovács Kinga Bernadett , Szalai Laura , Szakadáti Gyöngyi , Porkoláb Edit , Szalai Bence , Turu Gábor , Tóth András Dávid , Szekeres Mária , Hunyady László , Balla András
Published: December 2021

Activation of the type I angiotensin receptor (AT1-R) in vascular smooth muscle cells (VSMCs) plays a crucial role in the regulation of blood pressure; however, it is also responsible for the development of pathological conditions such as vascular remodeling, hypertension and atherosclerosis. Stimulation of the VSMC by angiotensin II (AngII) promotes a broad variety of biological effects, including gene expression changes. In this paper, we have taken an integrated approach in which an analysis of AngII-induced gene expression changes has been combined with the use of small-molecule inhibitors and lentiviral-based gene silencing, to characterize the mechanism of signal transduction in response to AngII stimulation in primary rat VSMCs. We carried out Affymetrix GeneChip experiments to analyze the effects of AngII stimulation on gene expression; several genes, including DUSP5, DUSP6, and DUSP10, were identified as upregulated genes in response to stimulation. Since various dual-specificity MAPK phosphatase (DUSP) enzymes are important in the regulation of mitogen-activated protein kinase (MAPK) signaling pathways, these genes have been selected for further analysis. We investigated the kinetics of gene-expression changes and the possible signal transduction processes that lead to altered expression changes after AngII stimulation. Our data shows that the upregulated genes can be stimulated through multiple and synergistic signal transduction pathways. We have also found in our gene-silencing experiments that epidermal growth factor receptor (EGFR) transactivation is not critical in the AngII-induced expression changes of the investigated genes. Our data can help us understand the details of AngII-induced long-term effects and the pathophysiology of AT1-R. Moreover, it can help to develop potential interventions for those symptoms that are induced by the over-functioning of this receptor, such as vascular remodeling, cardiac hypertrophy or atherosclerosis.
Clever Experimental Designs: Shortcuts for Better iPSC Differentiation
Authors: Yasui Ryota , Sekine Keisuke , Taniguchi Hideki
Published: December 2021

For practical use of pluripotent stem cells (PSCs) for disease modelling, drug screening, and regenerative medicine, the cell differentiation process needs to be properly refined to generate end products with consistent and high quality. To construct and optimize a robust cell-induction process, a myriad of cell culture conditions should be considered. In contrast to inefficient brute-force screening, statistical design of experiments (DOE) approaches, such as factorial design, orthogonal array design, response surface methodology (RSM), definitive screening design (DSD), and mixture design, enable efficient and strategic screening of conditions in smaller experimental runs through multifactorial screening and/or quantitative modeling. Although DOE has become routinely utilized in the bioengineering and pharmaceutical fields, the imminent need of more detailed cell-lineage specification, complex organoid construction, and a stable supply of qualified cell-derived material requires expedition of DOE utilization in stem cell bioprocessing. This review summarizes DOE-based cell culture optimizations of PSCs, mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and Chinese hamster ovary (CHO) cells, which guide effective research and development of PSC-derived materials for academic and industrial applications.
LIM Kinases in Osteosarcoma Development
Authors: Brion Régis , Regnier Laura , Mullard Mathilde , Amiaud Jérome , Rédini Françoise , Verrecchia Franck
Published: December 2021

Tumorigenesis is a long-term and multistage process that often leads to the formation of metastases. During this pathological course, two major events appear to be crucial: primary tumour growth and metastatic expansion. In this context, despite research and clinical advances during the past decades, bone cancers remain a leading cause of death worldwide among paediatric cancer patients. Osteosarcomas are the most common malignant bone tumours in children and adolescents. Notwithstanding advances in therapeutic treatments, many patients succumb to these diseases. In particular, less than 30% of patients who demonstrate metastases at diagnosis or are poor responders to chemotherapy survive 5 years after initial diagnosis. LIM kinases (LIMKs), comprising LIMK1 and LIMK2, are common downstream effectors of several signalization pathways, and function as a signalling node that controls cytoskeleton dynamics through the phosphorylation of the cofilin family proteins. In recent decades, several reports have indicated that the functions of LIMKs are mainly implicated in the regulation of actin microfilament and the control of microtubule dynamics. Previous studies have thus identified LIMKs as cancer-promoting regulators in multiple organ cancers, such as breast cancer or prostate cancer. This review updates the current understanding of LIMK involvement in osteosarcoma progression.
BDP1 Variants I1264M and V1347M Significantly Associated with Clinical Outcomes of Pediatric Neuroblastoma Patients Imply a New Prognostic Biomarker: A 121-Patient Cancer Genome Study
Authors: Li Xiaoqing , Sun Lan , Stucky Andres , Tu Lingli , Cai Jin , Chen Xuelian , Wu Zhongjun , Jiang Xuhong , Li Shengwen Calvin
Published: December 2021

Background: Neuroblastoma (N.B.) is the most common tumor in children. The gene BDP1 (B Double Prime 1) plays a role in cancers but is less known in N.B. Thus, we conducted this study to investigate the value of BDP1 mutations in N.B. prognosis. Methods: A dataset of 121 NB patients from the Cancer Genome Atlas database was used to analyze BDP1 gene mutations by RNA sequencing. Kaplan-Meier estimates were performed for overall survival (O.S.) analysis on BDP1 variants, and Cox’s proportional hazards regression model was used for multivariate analysis. Results: In 121 NB patients, we identified two variants of BDP1 associated with N.B., located at chr5:71511131 and chr5:71510884. The prevalence of these BDP1 variants, I1264M and V1347M, was 52.9% (64/121) and 45.5% (55/121), respectively. O.S. analysis showed a significant difference between subgroups with or without BDP1 variants (p < 0.05). Multivariate analysis further revealed that BDP1ariants were independent prognostic variables in N.B. (p < 0.05). Conclusion: Our results suggest BDP1 variants are associated with significantly improved clinical outcomes in N.B., thus providing clinicians with a new tool.
Political-Optimizer-Based Energy-Management System for Microgrids
Authors: Suresh Vishnu , Jasinski Michal , Leonowicz Zbigniew , Kaczorowska Dominika , J. Jithendranath , Reddy K. Hemachandra
Published: December 2021

This paper presents an energy-management strategy based on a recently introduced Political Optimizer (PO) for a microgrid installation at Wroclaw University of Science and Technology. The aim of the study is to check the effectiveness of two recently introduced meta-heuristic algorithms at power-system-operations planning. The optimization algorithms were compared with other conventional meta-heuristics wherein performance tests were carried out by minimizing costs in an IEEE 30-bus system. The best performing algorithm was then used to minimize the Levelized Cost of Energy (LCOE) in a microgrid consisting of renewable energy sources such as solar PV panels, a micro-hydro power plant, a fuel cell with a hydrogen storage tank and a Li-ion storage unit.
Factors Affecting Students’ Acceptance of Mobile Learning Application in Higher Education during COVID-19 Using ANN-SEM Modelling Technique
Authors: Almaiah Mohammed , Al-lozi Enas , Al-Khasawneh Ahmad , Shishakly Rima , Nachouki Mirna
Published: December 2021

Due to the COVID-19 pandemic, most universities around the world started to employ distance-learning tools. To cope with these emergency conditions, some universities in Jordan have developed “mobile learning platforms” as a new tool for distance teaching and learning for students. This experience in Jordan is still new and needs to be evaluated in order to identify its advantages and challenges. Therefore, this study aims to investigate students’ perceptions about mobile learning platforms as well as to identify the crucial factors that influence students’ use of mobile learning platforms. An online quantitative survey technique using Twitter was employed to collect the data. A two-staged ANN-SEM modelling technique was adopted to analyze the causal relationships among constructs in the research model. The results of the study indicate that content quality and service quality significantly influenced perceived usefulness of mobile learning platforms. In addition, perceived ease of use and perceived usefulness significantly influenced behavioral intention to use mobile learning platforms. The study findings provide useful suggestions for decision makers, service providers, developers, and designers in the ministry of education as to how to assess and enhance mobile learning platform quality and understanding of multidimensional factors for effectively using mobile learning platforms.
Generation of Localised Vertical Streams in Unstable Stratified Atmosphere
Authors: Onishchenko Oleg , Fedun Viktor , Ballai Istvan , Kryshtal Aleksandr , Verth Gary
Published: December 2021

A new model of axially symmetric concentrated vortex generation was developed herein. In this work, the solution of a nonlinear equation for internal gravity waves in an unstable stratified atmosphere was obtained and analysed in the framework of ideal hydrodynamics. The related expressions for the velocities in the inner and outer regions of the vortex were described by Bessel functions and modified zeroth-order Bessel functions. The proposed new nonlinear analytical model allows the study of the structure and dynamics of vortices in the radial region. The formation of jets (i.e., structures elongated in the vertical direction with finite components of the poloidal (radial and vertical) velocities that grow exponentially in time in an unstable stratified atmosphere) was also analysed. The characteristic growth time was determined by the inverse growth rate of instability. It is shown that a seed vertical vorticity component may be responsible for the formation of vortices with a finite azimuthal velocity.
Evaluation of Glycogen Storage Patients: Report of Twelve Novel Variants and New Clinical Findings in a Turkish Population
Authors: Ersoy Melike , Uyanik Bulent , Gedikbasi Asuman
Published: December 2021

Glycogen storage diseases (GSDs) are clinically and genetically heterogeneous disorders that disturb glycogen synthesis or utilization. Although it is one of the oldest inherited metabolic disorders, new genetic methods and long-time patient follow-ups provide us with unique insight into the genotype–phenotype correlations. The aim of this study was to share the phenotypic features and molecular diagnostic results that include new pathogenic variants in our GSD cases. Twenty-six GSD patients were evaluated retrospectively. Demographic data, initial laboratory and imaging features, and current findings of the patients were recorded. Molecular analysis results were classified as novel or previously defined variants. Novel variants were analyzed with pathogenicity prediction tools according to American College of Medical Genetics and Genomics (ACGM) criteria. Twelve novel and rare variants in six different genes were associated with the disease. Hearing impairment in two patients with GSD I, early peripheral neuropathy after liver transplantation in one patient with GSD IV, epilepsy and neuromotor retardation in three patients with GSD IXA were determined. We characterized a heterogeneous group of all diagnosed GSDs over a 5-year period in our institution, and identified novel variants and new clinical findings. It is still difficult to establish a genotype–phenotype correlation in GSDs.
Modelling and Mapping Total and Bioaccessible Arsenic and Lead in Stoke-on-Trent and Their Relationships with Industry
Authors: Wragg Joanna , Cave Mark
Published: December 2021

This study was based on a geochemical soil survey of Stoke-on-Trent in the UK of 747 surface soil samples analysed for 53 elements. A subset of 50 of these soil samples were analysed for their bioaccessible As and Pb content using the Unified Barge Method. Random Forest modelling, using the total element data as predictor variables, was used to predict bioaccessible As and Pb for all 747 samples. Random Forest modelling, using inverse distance weighed predictors and bedrock and superficial geology, was also used to map both total and bioaccessible As and Pb on a 400 × 400 spatial prediction grid with a 50 m resolution. The predicted bioaccessible As ranged from ca. 1 to 8 mg/kg and the total As ca. 8 to 45 mg/kg. The bioaccessible Pb and the total Pb both covered the range ca. 16–1200 mg/kg, with the highest values for both forms of Pb showing similar spatial distributions. Predictor variable importance and information on past industry suggest that the source of both of these elements is driven by anthropogenic causes.
Risk Assessment of Alpine Skiing Events Based on Knowledge Graph: A Focus on Meteorological Conditions
Authors: Wang Muhua , Zhang Xueying , Feng Deen , Wang Yipeng , Tang Wei , Ye Peng
Published: December 2021

The alpine skiing event is particularly vulnerable to changes in meteorological conditions as a winter sport held outdoors. The commonly used risk assessment methods cannot be inflexible and cannot be dynamically adjusted to combine multiple risk factors and actual conditions. A knowledge graph can organize data resources in the risk domain as structured knowledge systems. This paper combines a knowledge graph and risk assessment to effectively assess the risk status. First of all, we introduce the relevant literature review of sports event risk assessment, combining the characteristics of alpine skiing events. Then, we summarize the risk types of alpine skiing events and related risk knowledge. Secondly, a model is proposed to introduce an event risk assessment model based on the RippleNet framework combined with the characteristics of large-scale sports events. Moreover, the validity of the model is verified. The results show that the RippleNet-based event risk assessment model can be used to assess the risk of alpine skiing events. In order to effectively deal with the large-scale sports events that occur with a variety of risks, the smooth implementation of large-scale sports events provides a strong guarantee.
The Impact of the FilmArray-Based Detection of Microbial Pathogens from Positive Blood Culture Vials on the Time to Optimal Antimicrobial Regimen in Intensive Care Units of the Helios University Clinic Wuppertal, Germany
Authors: Schumann Jannik , Johanns Ulrike , Ahmad-Nejad Parviz , Ghebremedhin Beniam , Woebker Gabriele
Published: December 2021

The role of empirical therapy and time to first effective treatment, including the antimicrobial stewardship program, are decisive in patients presenting with bloodstream infections (BSI). The FilmArray® Blood Culture Identification Panel (FA BCID 1.0) detects 24 bacterial and fungal pathogens as well as 3 resistance genes from positive blood cultures in approximately 70 min. In this paper, we evaluate the impact of the additional FA BCID analysis on the time to an optimal antimicrobial therapy and on the length of stay in the ICU, ICU mortality, and PCT level reduction. This retro-/prospective trial was conducted in BSI patients in the ICU at a German tertiary care hospital. A total of 179 individual patients with 200 episodes of BSI were included in the prospective intervention group, and 150 patients with 170 episodes of BSI in the retrospective control group. In the intervention group, BSI data were analyzed including the MALDI-TOF MS (matrix assisted laser desorption ionization time-of-flight mass spectrometry) and FA BCID results from January 2019 to August 2020; the data from the control group, including the MALDI-TOF results, were collected retrospectively from the year 2018. The effective and appropriate antimicrobial regimen occurred in a median of 17 hours earlier in the intervention versus control group (p = 0.071). Furthermore, changes in the antimicrobial regimens of the intervention group that did not immediately lead to an optimal therapy occurred significantly earlier by a median of 24 hours (p = 0.029). Surrogate markers, indicating an earlier recovery of the patients from the intervention group, such as length of stay at the ICU, duration of mechanical ventilation, or an earlier reduction in PCT level, were not significantly affected. However, mortality did not differ between the patient groups. A postulated reduction of the antimicrobial therapy, in those cases in which coagulase-negative Staphylococcus species were identified, did occur in the control group, but not in the intervention group (p = 0.041). The implementation of FA BCID into the laboratory workflow can improve patient care by optimizing antimicrobial regimen earlier in BSI patients as it provides rapid and accurate results for key pathogens associated with BSI, as well as important antimicrobial resistance markers, e.g., mecA or vanA.
Circulating IgG Levels in SARS-CoV-2 Convalescent Individuals in Cyprus
Authors: Mamais Ioannis , Malatras Apostolos , Papagregoriou Gregory , Giallourou Natasa , Kakouri Andrea C. , Karayiannis Peter , Koliou Maria , Christaki Eirini , Nikolopoulos Georgios K. , Deltas Constantinos
Published: December 2021

Long-term persistence and the heterogeneity of humoral response to SARS-CoV-2 have not yet been thoroughly investigated. The aim of this work is to study the production of circulating immunoglobulin class G (IgG) antibodies against SARS-CoV-2 in individuals with past infection in Cyprus. Individuals of the general population, with or without previous SARS-CoV-2 infection, were invited to visit the Biobank at the Center of Excellence in Biobanking and Biomedical Research of the University of Cyprus. Serum IgG antibodies were measured using the SARS-CoV-2 IgG and the SARS-CoV-2 IgG II Quant assays of Abbott Laboratories. Antibody responses to SARS-CoV-2 were also evaluated against participants’ demographic and clinical data. All statistical analyses were conducted in Stata 16. The median levels of receptor binding domain (RBD)-specific IgG in 969 unvaccinated individuals, who were reportedly infected between November 2020 and September 2021, were 432.1 arbitrary units (AI)/mL (interquartile range—IQR: 182.4–1147.3). Higher antibody levels were observed in older participants, males, and those who reportedly developed symptoms or were hospitalized. The RBD-specific IgG levels peaked at three months post symptom onset and subsequently decreased up to month six, with a slower decay thereafter. IgG response to the RBD of SARS-CoV-2 is bi-phasic with considerable titer variability. Levels of IgG are significantly associated with several parameters, including age, gender, and severity of symptoms.
Tuberculosis in the Elderly
Authors: Caraux-Paz Pauline , Diamantis Sylvain , de Wazières Benoit , Gallien Sébastien
Published: December 2021

The tuberculosis (TB) epidemic is most prevalent in the elderly, and there is a progressive increase in the notification rate with age. Most cases of TB in the elderly are linked to the reactivation of lesions that have remained dormant. The awakening of these lesions is attributable to changes in the immune system related to senescence. The mortality rate from tuberculosis remains higher in elderly patients. Symptoms of active TB are nonspecific and less pronounced in the elderly. Diagnostic difficulties in the elderly are common in many diseases but it is important to use all possible techniques to make a microbiological diagnosis. Recognising frailty to prevent loss of independence is a major challenge in dealing with the therapeutic aspects of elderly patients. Several studies report contrasting data about poorer tolerance of TB drugs in this population. Adherence to antituberculosis treatment is a fundamental issue for the outcome of treatment. Decreased completeness of treatment was shown in older people as well as a higher risk of treatment failure.
Impact of Psoriasis and Hidradenitis Suppurativa in Pregnancy, a Systematic Review
Authors: Ferrer-Alcala Maria-Angeles , Sánchez-Díaz Manuel , Arias-Santiago Salvador , Molina-Leyva Alejandro
Published: December 2021

Psoriasis and hidradenitis suppurativa (HS) are chronic inflammatory skin diseases that frequently develop in young women. The aim of this study is to evaluate how hidradenitis suppurativa and psoriasis impact women desiring to conceive, and their influence on fertility and gestation. A systematic review of articles dating from January 2015 to April 2021 was performed using the Scopus (Elsevier) database. The search terms were (psoriasis and (birth or pregnancy or fertility)) and ((hidradenitis suppurativa or acne inversa) and (birth or pregnancy or fertility)). The search was limited to human data. Systematic reviews, case reports, clinical practice guidelines, expert consensus and conference papers were excluded. The impact of HS on pregnancy includes an impaired desire for pregnancy, a decrease in fertility, the worsening of the disease during pregnancy and potential adverse events during pregnancy. Moreover, the pregnancy might imply a change on the treatment of HS. The impact of psoriasis on pregnancy includes a decrease in fertility, potential adverse events during pregnancy and an unpredictable evolution of the disease. Moreover, the pregnancy might imply a change on the treatment of psoriasis, although biologic therapies do not appear to increase the risk of adverse events. In conclusion, both HS and psoriasis have an impact on pregnancy. A decrease of fertility has been reported. Moreover, both diseases have an unpredictable evolution during pregnancy. Pregnant women who are under biologic therapy do not seem to have a higher rate of adverse events. Treatment of both conditions is usually halted during pregnancy since scientific evidence about their safety is not conclusive, or teratogenic risk has been proven.
Physical Exercise with or without Whole-Body Vibration in Breast Cancer Patients Suffering from Aromatase Inhibitor—Induced Musculoskeletal Symptoms: A Pilot Randomized Clinical Study
Authors: de Sire Alessandro , Lippi Lorenzo , Ammendolia Antonio , Cisari Carlo , Venetis Konstantinos , Sajjadi Elham , Fusco Nicola , Invernizzi Marco
Published: December 2021

In this study, we aimed to assess the safety and efficacy of physical exercise, with or without whole-body vibration (WBV), in patients with aromatase inhibitor-induced musculoskeletal symptoms (AIMSS). Eligible patients were adults (≥18 years) with a history of breast cancer and current AIMSS. Enrolled patients (n = 22) were randomly assigned 1:1 to receive physical exercise combined with WBV or sham WBV for 4 weeks. The primary endpoint was pain intensity measured by numerical pain rating scale (NPRS). The secondary endpoints were muscle strength, physical function, physical performance, and quality of life. The WBV group (mean age: 51.73 ± 10.73 years; body mass index (BMI): 25.56 ± 5.17 kg/m2) showed a statistically significant pain reduction (NPRS: 6.82 ± 1.17 vs. 5.73 ± 1.01; p = 0.031), whereas patients in the sham WBV group (mean age: 58.55 ± 9.71 years; BMI: 27.31 ± 3.84 kg/m2), did not reach statistical significance (NPRS: 6.91 ± 2.02 vs. 5.91 ± 2.51; p = 0.07). Concurrently, muscle strength, physical performance, and quality of life significantly improved in both groups, without significant differences between groups. No dropouts and no side effects were recorded. Both patients and the physical therapist reported a high level of satisfaction with the intervention. Our findings suggest that physical exercise and WBV combination might be a safe therapeutic option for improving the rehabilitative management of patients with AIMSS.
Elimination of the Femoral Neck in Measuring Femoral Version Allows for Less Variance in Interobserver Reliability
Authors: Dimovski Radomir , Teitge Robert , Bolz Nicholas , Schafer Patrick , Bobba Vamsy , Vaidya Rahul
Published: December 2021

Background and Objectives: Producing consistent measures of femoral version amongst observers are necessary to allow for an assessment of version for possible corrective procedures. The purpose of this study was to compare two computed tomography (CT)-based techniques for the reliability of measuring femoral version amongst observers. Materials and Methods: Review was performed for 15 patients post-femoral nailing for comminuted (Winquist III and IV) femoral shaft fractures where CT scanograms were obtained. Two CT-based techniques were utilized to measure femoral version by five observers. Results: The mean femoral version, when utilizing a proximal line drawn down the center of the femoral head-neck through CT, was 9.50 ± 4.82°, while the method utilizing the head and shaft at lesser trochanter centers produced a mean version of 18.73 ± 2.69°. A significant difference was noted between these two (p ≤ 0.001). The method of measuring in the center of the femoral head and neck produced an intraclass correlation coefficient (ICC) of 0.960 with a 95% confidence interval lower bound of 0.909 and upper bound of 0.982. For the method assessing version via the center of the head and shaft at the lesser trochanter region, the ICC was 0.993 with a 95% confidence interval lower bound of 0.987 and an upper bound of 0.996. Conclusions: The method of measuring version proximally through a CT image of the femoral head–neck versus overlaying the femoral head with the femoral shaft at the most prominent aspect of the lesser trochanter produces differing version measurements by roughly 10° while yielding an almost perfect interobserver reliability in the new technique. Both techniques result in significantly high interobserver reliability.
Mixed Matrix Membranes Based on Torlon® and ZIF-8 for High-Temperature, Size-Selective Gas Separations
Authors: De Pascale Matilde , Benedetti Francesco Maria , Lasseuguette Elsa , Ferrari Maria-Chiara , Papchenko Kseniya , Degli Esposti Micaela , Fabbri Paola , De Angelis Maria Grazia
Published: December 2021

Torlon® is a thermally and plasticization-resistant polyamide imide characterized by low gas permeability at room temperature. In this work, we aimed at improving the polymer performance in the thermally-enhanced He/CO2 and H2/CO2 separations, by compounding Torlon® with a highly permeable filler, ZIF-8, to fabricate Mixed Matrix Membranes (MMMs). The effect of filler loading, gas size, and temperature on the MMMs permeability, diffusivity, and selectivity was investigated. The He permeability increased by a factor of 3, while the He/CO2 selectivity decreased by a factor of 2, when adding 25 wt % of ZIF-8 at 65 °C to Torlon®; similar trends were observed for the case of H2. The MMMs permeability and size-selectivity were both enhanced by temperature. The behavior of MMMs is intermediate between the pure polymer and pure filler ones, and can be described with models for composites, indicating that such materials have a good polymer/filler adhesion and their performance could be tailored by acting on the formulation. The behavior observed is in line with previous investigations on MMMs based on glassy polymers and ZIF-8, in similar conditions, and indicates that ZIF-8 can be used as a polymer additive when the permeability is a controlling aspect, with a proper choice of loading and operative temperature.
Alterations of Selected Hemorheological and Metabolic Parameters Induced by Physical Activity in Untrained Men and Sportsmen
Authors: Szanto Sandor , Mody Tobias , Gyurcsik Zsuzsanna , Babjak Laszlo Balint , Somogyi Viktoria , Barath Barbara , Varga Adam , Matrai Adam Attila , Nemeth Norbert
Published: December 2021

Optimal tissue oxygen supply is essential for proper athletic performance and endurance. It also depends on perfusion, so on hemorheological properties and microcirculation. Regular exercise is beneficial to the rheological status, depending on its type, intensity, and duration. We aimed to investigate macro and microrheological changes due to short, high-intensity exercise in professional athletes (soccer and ice hockey players) and untrained individuals. The exercise was performed on a treadmill ergometer during a spiroergometry examination. Blood samples were taken before and after exercise to analyze lactate concentration, hematological parameters, blood and plasma viscosity, and red blood cell (RBC) deformability and aggregation. Leukocyte, RBC and platelet counts, and blood viscosity increased with exercise, by the largest magnitude in the untrained group. RBC deformability slightly impaired after exercise, but showed better values in ice hockey versus soccer players. RBC aggregation increased with exercise, dominantly in ice hockey players. Lactate increased mostly in soccer players, and the respiratory exchange rate was the lowest in ice hockey players. Overall, short, high-intensity exercise altered macro and microrheological parameters, mostly in the untrained group. Significant differences were found between the two sports. The data can be useful in training status monitoring, selection, and in revealing the causes of physical loading symptoms.
Nickel Silicide Catalyst from Photovoltaic Waste for the Methanation Reaction
Authors: Bumba Jakub , Drinek Vladislav , Krystynik Pavel , Dytrych Pavel , Solcova Olga
Published: December 2021

A technology designed for recycling photovoltaic (PV) cells at the end of their life was successfully used for the preparation of a nickel silicide catalyst. PV cells were mixed with magnesium scrap to produce magnesium silicide (Mg2Si), with almost total conversion under optimized conditions (400 °C, 5 Pa, 25 min), in a constructed semi-open tubular reactor. Subsequently, magnesium silicide was hydrolyzed by 25% phosphoric acid to produce a mixture of silicon hydrides, which were utilized as chemical vapor deposition (CVD) precursors for the preparation of a nickel silicide catalyst. The activity and stability of the prepared catalyst was repeatedly tested for methanation reactions. It was verified that the nickel silicide catalyst showed an approximately 20% higher activity for the methanation reactions compared to the commonly used nickel catalyst.
Recently Discovered Thick Bentonite Bed Hosted by the Lithothamnium Limestones (Badenian) in the Polish Part of the Carpathian Foredeep: The Evidence for Volcanic Origin
Authors: Górniak Katarzyna , Szydłak Tadeusz , Wyszomirski Piotr , Gaweł Adam , Niemiec Małgorzata
Published: December 2021

In this paper, we discuss the hypothesis on the volcanic origin of the precursor sediments for a thick (0.6 m) clay bed, hosted by the sequence of lithothamnium limestones of the Pińczów Formation. Combined X-ray powder diffraction, imaging methods (optical and electron microscopy), and chemical analysis were used to document the volcanic markers, which were preserved in the rock studied. The results obtained show that the clay bed discussed is bentonite in origin. This bentonite, which can be called Drugnia Rządowa bentonite, is composed almost entirely of montmorillonite with little admixtures of quartz and biotite. A small amount of calcite is present, but only in the top of the bed. Despite that, the bentonite contains nothing but clay material—it is a model example of entirely altered pyroclastic rock, which retains texture originally developed in volcanic glass fragments and reveals the preserved original features of the precursor fallout pyroclastic deposits (rhyolitic in character). The thick bentonite beds, discovered for the first time within the Badenian lithothamnium limestones of the Pińczów Formation, can be considered as a record of a violent, explosive volcanic event related to the closure of the Outer Carpathian basin and the development of the Carpathian Foredeep.
NMR Spectral Characteristics of Ultrahigh Pressure High Temperature Impact Glasses of the Giant Kara Crater (Pay-Khoy, Russia)
Authors: Lyutoev Vladimir , Shumilova Tatyana , Mazur Anton , Tolstoy Peter
Published: December 2021

In this study, we carried out the analysis of the impact melt vein glasses from the Kara impact crater (Russia) in comparison to low-pressure impact melt glasses (tektites) of the Zhamanshin crater (Kazakhstan). 27Al, 23Na, and 29Si MAS NMR spectra of the samples of these glasses were analyzed. The samples of the natural glass contained inclusions of crystalline phases, paramagnetic elements that greatly complicate and distort the NMR signals from the glass phase itself. Taking into account the Mossbauer distribution of Fe in these glasses, the analysis of the spectra of MAS NMR of glass network-former (Si, Al) and potential network-modifiers (Na) of nuclei leads to the conclusion that the Kara impact melt vein glasses are characterized by complete polymerization of (Si,Al)O4 tetrahedral structural units. The NMR features of the glasses are consistent with the vein hypothesis of their formation under conditions of high pressures and temperatures resulting in their fluidity, relatively slow solidification with partial melt differentiation, polymerization, and precipitation of mineral phases as the impact melt cools. The 70 Ma stability of the Kara impact vein glass can be explained by the stabilization of the glass network with primary fine-dispersed pyroxene and coesite precipitates and by the high polymerization level of the impact glass.
High-Resolution UPLC-MS Profiling of Anthocyanins and Flavonols of Red Cabbage (Brassica oleracea L. var. capitata f. rubra DC.) Cultivated in Egypt and Evaluation of Their Biological Activity
Authors: Mansour Khaled Ahmed , Moustafa Sherifa Fahmy , Abdelkhalik Soad Mohamed
Published: December 2021

In this paper, biological investigations and a high-resolution UPLC-PDA-ESI-qTOF-HRMS technique were employed for Brassica oleracea L. var. capitata f. rubra DC. (red cabbage) of the family Brassicaceae (Cruciferae), cultivated in Egypt, for the first time. The positive ionization mode is usually performed to identify anthocyanins. However, this technique cannot differentiate between anthocyanins and corresponding non-anthocyanin polyphenols. Thus, the negative ionization mode was also used, as it provided a series of characteristic ions for the MS analysis of anthocyanins. This helped in identifying five kaempferol derivatives for the first time in red cabbage, as well as nine—previously reported—anthocyanins. For the biological investigations, the acidified methanolic extract of fresh leaves and the methanolic extract of air-dried powdered leaves were examined for their antioxidant, antimicrobial, and anticancer activities. The freshly prepared phenolic extract was proven to be more biologically potent. Statistical significance was determined for its anticancer activity in comparison with standard doxorubicin.
Easy Expression and Purification of Fluorescent N-Terminal BCL11B CCHC Zinc Finger Domain
Authors: Susemihl Anne , Nagel Felix , Grabarczyk Piotr , Schmidt Christian A. , Delcea Mihaela
Published: December 2021

Zinc finger proteins play pivotal roles in health and disease and exert critical functions in various cellular processes. A majority of zinc finger proteins bind DNA and act as transcription factors. B-cell lymphoma/leukemia 11B (BCL11B) represents one member of the large family of zinc finger proteins. The N-terminal domain of BCL11B was shown to be crucial for BCL11B to exert its proper function by homodimerization. Here, we describe an easy and fast preparation protocol to yield the fluorescently tagged protein of the recombinant N-terminal BCL11B zinc finger domain (BCL11B42-94) for in vitro studies. First, we expressed fluorescently tagged BCL11B42-94 in E. coli and described the subsequent purification utilizing immobilized metal ion affinity chromatography to achieve very high yields of a purified fusion protein of 200 mg/L culture. We proceeded with characterizing the atypical zinc finger domain using circular dichroism and size exclusion chromatography. Validation of the functional fluorescent pair CyPet-/EYFP-BCL11B42-94 was achieved with Förster resonance energy transfer. Our protocol can be utilized to study other zinc finger domains to expand the knowledge in this field.