bluuebunny/crossref_metadata_2025 huggingface dataset:
Nonlinear Dendritic Coincidence Detection for Supervised Learning
Authors: Schubert Fabian , Gros Claudius
Published: August 2021

Cortical pyramidal neurons have a complex dendritic anatomy, whose function is an active research field. In particular, the segregation between its soma and the apical dendritic tree is believed to play an active role in processing feed-forward sensory information and top-down or feedback signals. In this work, we use a simple two-compartment model accounting for the nonlinear interactions between basal and apical input streams and show that standard unsupervised Hebbian learning rules in the basal compartment allow the neuron to align the feed-forward basal input with the top-down target signal received by the apical compartment. We show that this learning process, termed coincidence detection, is robust against strong distractions in the basal input space and demonstrate its effectiveness in a linear classification task.
Long Noncoding RNAs in Neurodegenerative Diseases: Pathogenesis and Potential Implications as Clinical Biomarkers
Authors: Zhang Meng , He Ping , Bian Zhigang
Published: August 2021

Neurodegenerative diseases (NDDs), including Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), and amyotrophic lateral sclerosis (ALS), are progressive and ultimately fatal. NDD onset is influenced by several factors including heredity and environmental cues. Long noncoding RNAs (lncRNAs) are a class of noncoding RNA molecules with: (i) lengths greater than 200 nucleotides, (ii) diverse biological functions, and (iii) highly conserved structures. They directly interact with molecules such as proteins and microRNAs and subsequently regulate the expression of their targets at the genetic, transcriptional, and post-transcriptional levels. Emerging studies indicate the important roles of lncRNAs in the progression of neurological diseases including NDDs. Additionally, improvements in detection technologies have enabled quantitative lncRNA detection and application to circulating fluids in clinical settings. Here, we review current research on lncRNAs in animal models and patients with NDDs. We also discuss the potential applicability of circulating lncRNAs as biomarkers in NDD diagnostics and prognostics. In the future, a better understanding of the roles of lncRNAs in NDDs will be essential to exploit these new therapeutic targets and improve noninvasive diagnostic methods for diseases.
Identification of Candidate Biomarkers and Prognostic Analysis in Colorectal Cancer Liver Metastases
Authors: Zhang Tianhao , Yuan Kaitao , Wang Yingzhao , Xu Mingze , Cai Shirong , Chen Chuangqi , Ma Jinping
Published: August 2021

Background

Colorectal cancer (CRC), one of the most common malignant tumors worldwide, has a high mortality rate, especially for patients with CRC liver metastasis (CLM). However, CLM pathogenesis remains unclear.

Methods

We integrated multiple cohort datasets and databases to clarify and verify potential key candidate biomarkers and signal transduction pathways in CLM. GEO2R, DAVID 6.8, ImageGP, STRING, UALCAN, ONCOMINE, THE HUMAN PROTEIN ATLAS, GEPIA 2.0, cBioPortal, TIMER 2.0, DRUGSURV, CRN, GSEA 4.0.3, FUNRICH 3.1.3 and R 4.0.3 were utilized in this study.

Results

Sixty-three pairs of matched colorectal primary cancer and liver metastatic gene expression profiles were screened from three gene expression profiles (GSE6988, GSE14297 and GSE81558). Thirty-one up-regulated genes and four down-regulated genes were identified from these three gene expression profiles and verified by another gene expression profiles (GSE 49355) and TCGA database. Two pathways (IGFBP-IGF signaling pathway and complement-coagulation cascade), eighteen key differentially expressed genes (DEGs), six hub genes (SPARCL1, CDH2, CP, HP, TF and SERPINA5) and two biomarkers (CDH2 and SPARCL1) with significantly prognostic values were screened by multi-omics data analysis and verified by Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) cohort.

Conclusions

In this study, we identified a robust set of potential candidate biomarkers in CLM, which would provide potential value for early diagnosis and prognosis, and would promote molecular targeting therapy for CRC and CLM.
A Holistic Assessment of Polyethylene Fiber Ingestion in Larval and Juvenile Japanese Medaka Fish
Authors: DiBona Elizabeth , Pinnell Lee J. , Heising-Huang Annika , Geist Simon , Turner Jeffrey W. , Seemann Frauke
Published: August 2021

Microplastic pollution is of public concern for global environmental health, aquaculture, and fisheries. Toxicity studies have shown that microplastic ingestion may cause intestinal damage, microbiota dysbiosis, and disturb the lipid and energy metabolism in fish. To determine the impact of environmentally relevant, chronic, low dose microplastic fibers on fish health, medaka larvae, and juveniles were exposed to five concentrations of polyethylene (PE) fibers for 21 days through the feed. Fish growth and condition were assessed to determine the overall impact on fish health. To identify impaired energy intake, the gastrointestinal tract (GIT) integrity was evaluated at the molecular and cellular levels. Microbiota analysis was performed by comparing the top seven most abundant phyla present in both larval and juvenile fish exposed to 0, 1.5, and 3 PE fibers/fish/day. A shift in the phyla Proteobacteria and Bacteroidetes were observed. Larval samples demonstrated decreased proteobacteria abundance, while juvenile samples displayed an increase in abundance. Relative gene expression of key digestive genes from GIT tissue was quantified using real time-quantitative polymerase chain reaction. An effect on digestive gene expression potentially affecting nutrient absorption and antioxidant production was indicated via a significant decrease of solute carrier family 6 member 6 expression in larvae exposed to 6 fibers/fish/day. No significant molecular changes were observed in juvenile GIT tissue, although a non-monotonous dose-response was observed. GIT morphology was analyzed using histomorphological observations of the GIT mucus and cell types. No significant impairment of the GIT epithelial layers was observed in larvae or juveniles. To assess growth and condition, Fulton’s condition factor was measured. No differences were observed in larval or juvenile growth. Comparisons of different developmental stages allowed for identifying vulnerable developmental stages for microplastic exposure; larvae were more susceptible to molecular changes, while shifts in juvenile microbial communities were similar to changes reported post-polystyrene microplastic sphere exposure. This study is one of the first to provide toxicological data on the risk of PE fiber ingestion during fish development stages. Results indicate no imminent threat to fish condition at current measured environmental levels of microplastics; however, close monitoring of vital spawning grounds for commercially important fishes is recommended.
Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera
Authors: Yao Shuangyan , Zhang Yunhui , Chang Yanpeng , Li Xiang , Zhao Wenli , An Shiheng
Published: August 2021

Pyruvate kinase (PYK) is a speed-limited enzyme of glycolysis that catalyzes the formation of pyruvate, and plays an important role in acetyl-CoA synthesis. The acetyl-CoA is the precursor of sex pheromone biosynthesis inHelicoverpa armigera. However, the role of PYK in sex pheromone biosynthesis remains elusive. Here,PYKinH. armigera(HaPYK) was found to be highly expressed in the pheromone glands (PGs). The developmental expression profile ofHaPYKwas consistent with the fluctuation of sex pheromone release. Function analysis revealed that the knockdown ofHaPYKled to a decrease in the levels of pyruvic acid and acetyl-CoA in PGs, which in turn caused a significant decrease in cis-11-hexadecenal (Z11-16: Ald) production, female capability to attract males, and mating frequency. Further study demonstrated that sugar feeding (5% sugar) increased the transcription and enzyme activity of HaPYK, thereby facilitating sex pheromone biosynthesis. Moreover, pheromone biosynthesis activating neuropeptide (PBAN) upregulated HaPYK activity through protein kinase C (PKC), as shown by PKC-specific inhibitor analysis. Altogether, our results revealed that PBAN activated HaPYK by Ca2+/PKC, thereby regulating the synthesis of pyruvate and subsequent acetyl-CoA, ensuring the supply of sex pheromone precursor, and finally facilitating sex pheromone biosynthesis and mating behavior.
Arterial Load and Norepinephrine Are Associated With the Response of the Cardiovascular System to Fluid Expansion
Authors: Nguyen Maxime , Mallat Jihad , Marc Julien , Abou-Arab Osama , Bouhemad Bélaïd , Guinot Pierre-Grégoire
Published: August 2021

Background

Fluid responsiveness has been extensively studied by using the preload prism. The arterial load might be a factor modulating the fluid responsiveness. The norepinephrine (NE) administration increases the arterial load and modifies the vascular properties. The objective of the present study was to determine the relationship between fluid responsiveness, preload, arterial load, and NE use. We hypothesized that as a preload/arterial load, NE use may affect fluid responsiveness.

Methods

The retrospective multicentered analysis of the pooled data from 446 patients monitored using the transpulmonary thermodilution before and after fluid expansion (FE) was performed. FE was standardized between intensive care units (ICUs). The comparison of patients with and without NE at the time of fluid infusion was performed. Stroke volume (SV) responsiveness was defined as an increase of more than 15% of SV following the FE. Pressure responsiveness was defined as an increase of more than 15% of mean arterial pressure (MAP) following the FE. Arterial elastance was used as a surrogate for the arterial load.

Results

A total of 244 patients were treated with NE and 202 were not treated with NE. By using the univariate analysis, arterial elastance was correlated to SV variations with FE. However, the SV variations were not associated with NE administration (26 [15; 46]% vs. 23 [10; 37]%, p = 0.12). By using the multivariate analysis, high arterial load and NE administration were associated with fluid responsiveness. The association between arterial elastance and fluid responsiveness was less important in patients treated with NE. Arterial compliance increased in the absence of NE, but it did not change in patients treated with NE (6 [−8; 19]% vs. 0 [−13; 15]%, p = 0.03). The changes in total peripheral and arterial elastance were less important in patients treated with NE (−8 [−17; 1]% vs. −11 [−20; 0]%, p < 0.05 and −10 [−19; 0]% vs. −16 [−24; 0]%, p = 0.01).

Conclusion

The arterial load and NE administration were associated with fluid responsiveness. A high arterial load was associated with fluid responsiveness. In patients treated with NE, this association was lower, and the changes of arterial load following FE seemed to be driven mainly by its resistive component.
Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics
Authors: Chen Peilei , Wei Xiaoyan , Qi Qianting , Jia Wenjing , Zhao Mingwei , Wang Huina , Zhou Yanqing , Duan Hongying
Published: August 2021

Rehmannia glutinosa has important medicinal value; terpenoid is one of the main active components in R. glutinosa. In this study, iTRAQ technique was used to analyze the relative abundance of proteins in roots of R. glutinosa, and 6,752 reliable proteins were quantified. GO enrichment results indicated that most proteins were involved in metabolic process or cellular process, 57.63% proteins had catalytic activity, and 65.80% proteins were enriched in membrane-bounded organelle. In roots of R. glutinosa, there were 38 KEGG enrichments with significance, more DEPs were found in some pathways, especially the proteasome pathway and TCA cycle with 15.0% DEPs between elongation stage and expansion stage of roots. Furthermore, five KEGG pathways of terpenoid synthesis were found. Most prenyltransferases belong to FPP/GGPP synthase family, involved in terpenoid backbone biosynthesis, and all interacted with biotin carboxylase CAC2. Compared with that at the elongation stage, many prenyltransferases exhibited higher expression at the expansion stage or maturation stage of roots. In addition, eight FPP/GGPP synthase encoding genes were cloned from R. glutinosa, namely FPPS, FPPS1, GGPS, GGPS3, GGPS4, GGPS5, GPPS and GPPS2, introns were also found in FPPS, FPPS1, GGPS5 and GGPS2, and FPP/GPP synthases were more conservative in organisms, especially in viridiplantae, in which the co-occurrence of GPPS or GPPS2 was significantly higher in plants. Further analysis found that FPP/GGPP synthases of R. glutinosa were divided into three kinds, GGPS, GPPS and FPPS, and their gene expression was significantly diverse in different varieties, growth periods, or tissues of R. glutinosa. Compared with that of GGPS, the expression of GPPS and FPPS was much higher in R. glutinosa, especially at the expansion stage and maturation stage. Thus, the synthesis of terpenoids in roots of R. glutinosa is intricately regulated and needs to be further studied.
Effects of Sport Teaching on Students' Enjoyment and Fun: A Systematic Review and Meta-Analysis
Authors: Rodríguez Macías Manuel , Abad Robles Manuel Tomás , Giménez Fuentes-Guerra Francisco Javier
Published: August 2021

The methodology used in sport teaching influences not only the students' technical and tactical learning, but also psychological and social dimensions such as enjoyment. We aimed to analyze the effects of interventions using conventional and non-conventional sport teaching methodology on students' enjoyment/fun, through a systematic review and meta-analysis. The search was carried out following the PRISMA Statement in the databases of Web of Science, PubMed, Scopus, SportDiscus, Eric and PsycInfo. A total of 1,481 documents were obtained, with the addition of 5 more which were identified in the reference lists of the articles found in the databases. Eleven articles were considered to meet the inclusion criteria. The meta-analysis yielded a moderate effect size of 0.72, and a 95% CI from 0.48 to 0.96 in favor of non-conventional teaching methods, highlighting the model of sports education. Nevertheless, the heterogeneity of the interventions was large and the quality of the evidence, according to GRADE, was very low. In conclusion, the use of non-conventional models in sport teaching with the aim of increasing the enjoyment/fun of boys and girls is advised. These suggestions could be useful for teachers and sport coaches to increase the enjoyment/fun of their trainees during sport practice.
Effects of Experiential Learning Programmes on Adolescent Prosocial Behaviour, Empathy, and Subjective Well-being: A Systematic Review and Meta-Analysis
Authors: Chan Hannah Hoi-Kiu , Kwong Ho Yee Chloe , Shu Geisty Lin Feng , Ting Chung Yan , Lai Frank Ho-Yin
Published: August 2021

Introduction:Effective adolescent learning programmes can positively influence adolescent development and curb risky behaviour. By immersing learners in an experience, experiential learning motivates learners to reflect on the experience to transform and create new skills, attitudes and ways of thinking. However, evidence of its effectiveness in learning programs facilitating positive youth development is still lacking. The objective of this study is to (a) identify the effect of adolescent learning programmes on prosocial behaviour, empathy and subjective well-being, (b) compare the effectiveness of experiential learning programmes and non-experiential learning programmes on improving these three outcomes, and (c) evaluating the effects of age on the outcomes of adolescent learning programmes.

Methods:This study was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Randomised controlled trials of learning programmes for typically developing adolescents aged 8–25 in the past 15 years were identified, and assessed for quality with the Physiotherapy Evidence Database (PEDRO) scale. One thousand ninety-six records were screened with the inclusion and exclusion criteria, and 20 studies were adopted for this meta-analysis. The standardised mean difference and 95% confidence interval (CI) of the effect of experiential learning program on empathy, prosocial behaviour, and subjective well-being were examined. Sub-group analysis based on age was conducted to examine the effects of experiential learning on adolescents in different stages of life.

Results:Experiential learning programmes were more effective than non-experiential learning programmes in improving empathy [d = 0.65 (0.07, 1.23)] and subjective well-being [d = 0.46 (0.33, 0.59)]. The effect sizes of the three outcomes in non-experiential learning programmes were non-significant. Studies conducted on older adolescents had the most significant improvements in the three outcomes.

Conclusions:Results suggest the broader application of experiential learning in adolescent learning programmes for older adolescents in the future to promote positive youth development.
Household Air Pollution From Solid Cooking Fuel Combustion and Female Breast Cancer
Authors: Liu Tanxin , Chen Ru , Zheng Rongshou , Li Liming , Wang Shengfeng
Published: August 2021

Background: Women bear a large share of disease burden caused by household air pollution due to their great involvement in domestic activities. Pollutant emissions are believed to vary by exposure patterns such as cooking and space heating. Little is known about the independent effect of solid cooking fuel combustion on breast cancer risk. We aimed to examine the association of indoor coal and wood combustion for cooking with breast cancer risk.

Methods: During June 2004–July 2008, participants aged 30–79 from 10 diverse regions across China were enrolled in the China Kadoorie Biobank. Primary cooking fuel use information in up to three residences was self-reported at baseline. Multivariable logistic regression models yielded adjusted odds ratios (ORs) and 95% confidence intervals (CIs).

Results: A total of 290,396 female participants aged 30–79 were included in the main analysis. Compared with long-term clean fuel users, the fully adjusted ORs were 2.07 (95%CI: 1.37–3.13) for long-term coal users, 1.12 (95% CI: 0.72–1.76) for long-term wood users, and 0.98 (95% CI: 0.55–1.74) for those who used mixed solid fuels to cook. Those who had switched from solid to clean fuels did not have an excess risk of breast cancer (OR: 0.88, 95%CI 0.71–1.10).

Conclusion: Long-term solid fuel combustion for cooking may increase the risk of breast cancer. The strength of association is stronger among coal users than wood users. Targeted interventions are needed to accelerate the access to clean and affordable energy.
The Socioeconomic Impact of Surgical Site Infections
Authors: Piednoir Emmanuel , Robert-Yap Joan , Baillet Patrice , Lermite Emilie , Christou Niki
Published: August 2021

Précis: Surgical site infections are an ever-increasing phenomenon worldwide due to different factors. This brief report aimeds to highlight at a glance, for both physicians and political and institutional leaders, the economic burden of surgical site infections.

Objectives: This brief report aimed to highlight the economic burden of surgical site infections (SSIs).

Methods: A narrative review focusing on this subject has been carried out.

Results: Surgical site infections are responsible for generating important costs. In 2017, a French cohort highlighted a mean cost of each SSI treatment to be around €1,814; the same year, the Centers for Disease Control and Prevention guidelines evaluated the mean cost caused by SSI treatment to be from $10,443 to $25,546 per SSI. This cost depends on many factors including the patient himself and the type of surgery.

Conclusions: Prevention of the risk of infection is, therefore, a profitable concept for surgery that must be integrated within all healthcare managements worldwide.
Prevalence of Anti-Toxoplasma gondii and Anti-Brucella Spp. Antibodies in Pregnant Women From Mogadishu, Somalia
Authors: Yusuf Abdulkarim A. , Hassan-Kadle Ahmed A. , Ibrahim Abdalla M. , Hassan-Kadle Mohamed A. , Yasin Abdullahi M. , Khojaly Maha , Garcia João L. , Vieira Rafael F. C.
Published: August 2021

Toxoplasmosis and brucellosis are zoonotic diseases of worldwide distribution. They both cause abortion and infertility in human and animals. Limited data are available about these pathogens in Somali people and their animals. Hence, this study has evaluated the prevalence of anti-Toxoplasma gondii and anti-Brucella spp. antibodies in pregnant women in Mogadishu, Somalia. Serum samples from 307 pregnant women from Mogadishu, Somalia were tested for anti-T. gondii antibodies by Latex Agglutination Test (LAT) and anti-Brucella spp. antibodies by Rose Bengal Plate Test (RBPT) and a commercial competitive-ELISA (cELISA). A total of 119/307 (38.76%) pregnant women had a prior history of abortion. A total of 159/307 (51.79%; 95% CI: 46.2–57.35%) pregnant women were seroreactive for T. gondii by LAT at different stages of pregnancy. For Brucella spp., when RBPT and cELISA were combined 4/307 (1.30%; 95% CI: 0.36–3.30%) pregnant women were seroreactive to anti-Brucella spp. antibodies, being 2/307 (0.65%; 95% CI: 0.18–2.34%) by RBPT and 3/307 (0.98%; 95% CI: 0.33–2.83%) by cELISA. Two women were seroreactive for both agents. A high seropositivity to T. gondii and low seropositivity to Brucella spp. have been found in pregnant women from Mogadishu, Somalia. Considering the high number of abortions in the country associated to the fact that awareness on other zoonotic abortifacient pathogens in Somalis is very low, further studies should be conducted to evaluate the potential causes of abortions.
How Stress Affects Us
Authors: Ellis Stephanie , Bhathe Vishnu P. , Brennan Christina , Moynes Emily , Hellemans Kim G. C. , Landsman Sean J.
Published: August 2021

Stress is something we all experience in our daily lives. While we often think stress is a bad thing, some stress can be good for us, such as when we sense danger and run away from it. The moment the body senses something stressful, it immediately triggers a series of events to help us handle the stress. However, if our bodies are responding to stress all the time, it can be hard on our overall health. Therefore, it is important to maximize time spent exposing ourselves to “good” stress, minimize exposure to “bad” stress, and provide ourselves with lots of time to recover from any bad stress we might experience. Understanding how our bodies respond to stress and what conditions are best for our overall health is important for making healthy life choices.
The Diagnostic Yield of Cone-Beam Computed Tomography for Degenerative Changes of the Temporomandibular Joint in Dogs
Authors: McKay Rachel Marie , Vapniarsky Natalia , Hatcher David , Carr Nicole , Chen Shuai , Verstraete Frank J. M. , Cissell Derek D. , Arzi Boaz
Published: August 2021

Degenerative changes of the temporomandibular joint (DTMJ) may be diagnosed via cone - beam computed tomography (CBCT). However, despite advancement in CBCT imaging, correlation of DTMJ features identified on CBCT with gross and histological findings is currently limited. This study aimed to correlate CBCT findings of DTMJ of dogs with gross and histopathologic changes. Temporomandibular joints (TMJ) (n = 38) from fresh cadaver heads of asymptomatic dogs (n = 19) were examined radiologically, macroscopically, and microscopically. Association of CBCT - detected DTMJ changes with gross and histological findings were statistically evaluated via kappa statistics and ordinal logistic mixed-effects models. The radiological changes observed on CBCT included joint space narrowing, subchondral/cortical bone changes (i.e., erosions or lysis), osteophytes, and subchondral bone sclerosis. Upon macroscopic evaluation, the majority of examined specimens had mild changes with cartilage defects and osteophytes affecting <10% of the total articular surface area. Histopathologic changes comprised splitting and degeneration of the fibrous cartilage layers, subchondral bone exposure, subchondral bone sclerosis, focal subchondral bone lysis, and occasional cell death. Subchondral sclerosis was the most prevalent finding radiologically and histologically with a fair to excellent agreement. Importantly, the more severe the TMJ degenerative changes, the higher the agreement between CBCT and histology. Based on the correlative results of statistical analysis, CBCT was found to be a suitable modality to evaluate DTMJ.
Using Genomic Selection to Leverage Resources among Breeding Programs: Consortium-Based Breeding
Authors: Sneller Clay , Ignacio Carlos , Ward Brian , Rutkoski Jessica , Mohammadi Mohsen
Published: August 2021

Genomic selection has many applications within individual programs. Here, we discuss the benefits of forming a GS-based breeding consortium (GSC) among programs within the context of a recently formed a GSC of soft red winter wheat breeding programs. The GSC will genotype lines from each member breeding program (MBP) and conduct cooperative phenotyping. The primary GSC benefit is that each MBP can use GS to predict the local and broad value of all germplasm from all MBPs including lines in the early stages of testing, thus increasing the effective size of each MBP without significant new investment. We identified eight breeding aspects that are essential to GSC success and analyzed how our GSC fits those criteria. We identified a core of >5700 related lines from the MBPs that can serve in training populations. Germplasm from each MBP provided breeding value to other MBPs and program-specific adaption was low. GS accuracy was acceptable within programs but was low between programs when using training populations with little testing connectivity, but increased when using data from trials with high testing connectivity between MBPs. In response we initiated sparse-testing with a germplasm sharing scheme utilizing family relationship to connect our phenotyping of early-stage lines.
Enhancing Dissolution Rate and Antibacterial Efficiency of Azithromycin through Drug-Drug Cocrystals with Paracetamol
Authors: Ul Islam Noor , Khan Ezzat , Naveed Umar Muhammad , Shah Attaullah , Zahoor Muhammad , Ullah Riaz , Bari Ahmed
Published: August 2021

Cocrystallization is a promising approach to alter physicochemical properties of active pharmaceutical ingredients (hereafter abbreviated as APIs) bearing poor profile. Nowadays pharmaceutical industries are focused on preparing drug-drug cocrystals of APIs that are often prescribed in combination therapies by physicians. Physicians normally prescribe antibiotic with an analgesic/antipyretic drug to combat several ailments in a better and more efficient way. In this work, azithromycin (AZT) and paracetamol (PCM) cocrystals were prepared in 1:1 molar ratio using slow solvent evaporation method. The cocrystals were characterized by Fourier transform infrared (FTIR), Raman spectroscopy, powder X-ray diffraction (PXRD), differential scanning calorimeter (DSC), thermo gravimetric analysis (TGA) and high-performance liquid chromatography (HPLC). Vibrational spectroscopy and DSC confirmed that both APIs interact physically and showed chemical compatibility, while PXRD pattern of the starting material and products revealed that cocrystal have in a unique crystalline phase. The degree of hydration was confirmed by TGA analysis and result indicates monohydrate cocrystal formation. The HPLC analysis confirmed equimolar ratio of AZT:PCM in the cocrystal. The in vitro dissolution rate, saturation solubility, and antimicrobial activity were evaluated for AZT dihydrate and the resulting cocrystals. The cocrystals exhibited better dissolution rate, solubility and enhanced biological activities.
Remaining Useful Life Prediction of Broken Rotor Bar Based on Data-Driven and Degradation Model
Authors: Bejaoui Islem , Bruneo Dario , Xibilia Maria Gabriella
Published: August 2021

Rotating machines such as induction motors are crucial parts of most industrial systems. The prognostic health management of induction motor rotors plays an essential role in increasing electrical machine reliability and safety, especially in critical industrial sectors. This paper presents a new approach for rotating machine fault prognosis under broken rotor bar failure, which involves the modeling of the failure mechanism, the health indicator construction, and the remaining useful life prediction. This approach combines signal processing techniques, inherent metrics, and principal component analysis to monitor the induction motor. Time- and frequency-domains features allowing for tracking the degradation trend of motor critical components that are extracted from torque, stator current, and speed signals. The most meaningful features are selected using inherent metrics, while two health indicators representing the degradation process of the broken rotor bar are constructed by applying the principal component analysis. The estimation of the remaining useful life is then obtained using the degradation model. The performance of the prediction results is evaluated using several criteria of prediction accuracy. A set of synthetic data collected from a degraded Simulink model of the rotor through simulations is used to validate the proposed approach. Experimental results show that using the developed prognostic methodology is a powerful strategy to improve the prognostic of induction motor degradation.
Ready-to-Eat Sandwiches as Source of Pathogens Endowed with Antibiotic Resistance and Other Virulence Factors
Authors: Camellini Stefania , Iseppi Ramona , Condò Carla , Messi Patrizia
Published: August 2021

The aim of this study was to evaluate and characterize the bacterial load present in twenty-four Ready-To-Eat (RTE) sandwiches, purchased at refrigerated vending machines and supermarkets in the province of Modena (Italy). We isolated 54 bacterial strains, including pathogens of interest in food safety, such as Listeria, Staphylococcus, Enterococcus, Yersinia, Aeromonas and Acinetobacter spp. Phenotypic tests have been performed on these pathogens to detect the presence of virulence factors, such as gelatinase production and hemolytic capability. To test their antibiotic resistance features, the minimum inhibitory concentration (MIC) against eight commonly used antibiotics (Amikacin, Ciprofloxacin, Ampicillin, Oxacillin, Imipenem, Tetracycline, Erythromycin and Vancomycin) was also evaluated. The results showed that among the 54 isolates, fifty percent (50%) belonged to harmless microorganisms (Leuconostoc and Lactococcus), whereas the remaining fifty percent (50%) included pathogenic bacteria (Listeria ivanovii, Listeria monocytogenes, Staphylococcus aureus, Yersinia, and Citrobacter spp.), species responsible for pathologies often difficult to treat due to the presence of antibiotic resistance features. This study demonstrates the importance of thorough controls, both during the production and marketing of RTE food like sandwiches, to avoid reaching the infectious load and the onset of pathologies, particularly dangerous for old and immunocompromised patients.
Fast Pre-Diagnosis of Neoplastic Changes in Cytology Images Using Machine Learning
Authors: Caputa Jakub , Łukasik Daria , Wielgosz Maciej , Karwatowski Michał , Frączek Rafał , Russek Paweł , Wiatr Kazimierz
Published: August 2021

We present the experiment results to use the YOLOv3 neural network architecture to automatically detect tumor cells in cytological samples taken from the skin in canines. A rich dataset of 1219 smeared sample images with 28,149 objects was gathered and annotated by the vet doctor to perform the experiments. It covers three types of common round cell neoplasms: mastocytoma, histiocytoma, and lymphoma. The dataset has been thoroughly described in the paper and is publicly available. The YOLOv3 neural network architecture was trained using various schemes involving original dataset modification and the different model parameters. The experiments showed that the prototype model achieved 0.7416 mAP, which outperforms the state-of-the-art machine learning and human estimated results. We also provided a series of analyses that may facilitate ML-based solutions by casting more light on some aspects of its performance. We also presented the main discrepancies between ML-based and human-based diagnoses. This outline may help depict the scenarios and how the automated tools may support the diagnosis process.
Using FEM to Assess the Effect of Orthodontic Forces on Affected Periodontium
Authors: Luchian Ionut , Martu Maria-Alexandra , Tatarciuc Monica , Scutariu Mihaela Monica , Ioanid Nicoleta , Pasarin Liliana , Kappenberg-Nitescu Diana Cristala , Sioustis Ioana-Andreea , Solomon Sorina Mihaela
Published: August 2021

Orthodontic treatment in patients with no periodontal tissue breakdown vs. horizontal bone loss should be approached with caution even though it can bring significant benefits in terms of periodontal recovery and long-term success. We used the finite element method (FEM) to simulate various clinical scenarios regarding the periodontal involvement: healthy with no horizontal bone loss, moderate periodontal damage (33%) and severe horizontal bone loss (66%). Afterwards, forces of different magnitudes (0.25 N, 1 N, 3 N, and 5 N) were applied in order to observe the behavioral patterns. Through mathematical modeling, we recorded the maximum equivalent stresses (σ ech), the stresses on the direction of force application (σ c) and the displacements produced (f) in the whole tooth–periodontal ligament–alveolar bone complex with various degrees of periodontal damage. The magnitude of lingualization forces in the lower anterior teeth influences primarily the values of equivalent tension, then those of the tensions in the direction in which the force is applied, and lastly those of the displacement of the lower central incisor. However, in the case of the lower lateral incisor, it influences primarily the values of the tensions in the direction in which the force is applied, then those of equivalent tensions, and lastly those of displacement. Anatomical particularities should also be considered since they may contribute to increased periodontal risk in case of lingualization of the LLI compared to that of the LCI, with a potential emergence of the “wedge effect”. To minimize periodontal hazards, the orthodontic force applied on anterior teeth with affected periodontium should not exceed 1 N.
Biomechanical Characteristics for Identifying the Cutting Direction of Professional Soccer Players
Authors: Chen Yo , Lo Tang-Yun , Xu Fei , Chang Jia-Hao
Published: August 2021

(1) Background: To understand the movement characteristics of soccer players when cutting in two directions (45° for the right and left cutting movements) through biomechanical analysis of the lower limbs to identify the cutting direction of the attacker; (2) Methods: A motion analysis system was used to capture the movements of 12 male professional soccer players dribbling to the left and right. Kinematics of the players’ cutting were analyzed, and the paired t-test was used for statistics, with a significant level of α = 0.05; (3) Results: When cutting towards the right, the height of the hip joint during the run-up was low (effect size, ES = 0.41, p = 0.031) at 91.8 ± 7.0 cm. When cutting towards the left, the value was 94.6 ± 6.7 cm. While cutting, the front foot was abducted by 4.3 ± 4.0° at landing when cutting towards the right and adducted by 2.7 ± 5.1° when cutting towards the left (ES = 0.38, p = 0.003); (4) Conclusions: When the attacker carries out the cutting action while approaching the defender, the cutting direction may be predicted by observing the attacker’s hip and foot movements.
Low Ionosphere under Influence of Strong Solar Radiation: Diagnostics and Modeling
Authors: Srećković Vladimir A. , Šulić Desanka M. , Ignjatović Ljubinko , Vujčić Veljko
Published: August 2021

Solar flares (SFs) and intense radiation can generate additional ionization in the Earth’s atmosphere and affect its structure. These types of solar radiation and activity create sudden ionospheric disturbances (SIDs), affect electronic equipment on the ground along with signals from space, and potentially induce various natural disasters. Focus of this work is on the study of SIDs induced by X-ray SFs using very low frequency (VLF) radio signals in order to predict the impact of SFs on Earth and analyze ionosphere plasmas and its parameters. All data are recorded by VLF BEL stations and the model computation is used to obtain the daytime atmosphere parameters induced by this extreme radiation. The obtained ionospheric parameters are compared with results of other authors. For the first time we analyzed physics of the D-region—during consecutive huge SFs which continuously perturbed this layer for a few hours—in detail. We have developed an empirical model of the D-region plasma density and gave a simple approximative formula for electron density.
An Augmented Warning System for Pedestrians: User Interface Design and Algorithm Development
Authors: Tong Yourui , Jia Bochen , Bao Shan
Published: August 2021

Warning pedestrians of oncoming vehicles is critical to improving pedestrian safety. Due to the limitations of a pedestrian’s carrying capacity, it is crucial to find an effective solution to provide warnings to pedestrians in real-time. Limited numbers of studies focused on warning pedestrians of oncoming vehicles. Few studies focused on developing visual warning systems for pedestrians through wearable devices. In this study, various real-time projection algorithms were developed to provide accurate warning information in a timely way. A pilot study was completed to test the algorithm and the user interface design. The projection algorithms can update the warning information and correctly fit it into an easy-to-understand interface. By using this system, timely warning information can be sent to those pedestrians who have lower situational awareness or obstructed view to protect them from potential collisions. It can work well when the sightline is blocked by obstructions.
Application of Plasma Technology in Bioscience and Biomedicine
Authors: Boehm Daniela , Canal Cristina
Published: August 2021

Plasma technology has been an integral part of research in life sciences for decades through its role in the manufacture and modification of material surface characteristics of many common laboratory consumables, and it is still of interest in many fields, including the treatment of biomaterials and implants [...]
Specificity of Meteorological and Biometeorological Conditions in Central Europe in Centre of Urban Areas in June 2019 (Bydgoszcz, Poland)
Authors: Okoniewska Monika
Published: August 2021

The work describes diurnal meteorological and biometeorological conditions in June 2019 in the urban areas of Central Europe. UTCI, STI, Oh_H, WL, and OV indices were calculated based on 24-h data from Bydgoszcz (Poland) for hot days. The degree of risk connected with heat stress of different intensities, risk of hyperthermia, body water loss, and decreased oxygen volume was determined. The studies showed that June 2019 was an example of an extreme situation with a heatwave that generated high stress for the inhabitants of urban areas. The conditions were burdensome mostly due to “very strong” and “strong” heat stress and periodic risk of dehydration, situations that could quickly lead to overheating of the body and a decreased oxygen volume leading to stress.
Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk
Authors: Abdelsattar Abdallah S. , Safwat Anan , Nofal Rana , Elsayed Amera , Makky Salsabil , El-Shibiny Ayman
Published: August 2021

Food safety is very important in the food industry as most pathogenic bacteria can cause food-borne diseases and negatively affect public health. In the milk industry, contamination with Salmonella has always been a challenge, but the risks have dramatically increased as almost all bacteria now show resistance to a wide range of commercial antibiotics. This study aimed to isolate a bacteriophage to be used as a bactericidal agent against Salmonella in milk and dairy products. Here, phage ZCSE6 has been isolated from raw milk sample sand molecularly and chemically characterized. At different multiplicities of infection (MOIs) of 0.1, 0.01, and 0.001, the phage–Salmonella interaction was studied for 6 h at 37 °C and 24 h at 8 °C. In addition, ZCSE6 was tested against Salmonella contamination in milk to examine its lytic activity for 3 h at 37 °C. The results showed that ZCSE6 has a small genome size (<48.5 kbp) and belongs to the Siphovirus family. Phage ZCSE6 revealed a high thermal and pH stability at various conditions that mimic milk manufacturing and supply chain conditions. It also demonstrated a significant reduction in Salmonella concentration in media at various MOIs, with higher bacterial eradication at higher MOI. Moreover, it significantly reduced Salmonella growth (MOI 1) in milk, manifesting a 1000-fold decrease in bacteria concentration following 3 h incubation at 37 °C. The results highlighted the strong ability of ZCSE6 to kill Salmonella and control its growth in milk. Thus, ZCSE6 is recommended as a biocontrol agent in milk to limit bacterial growth and increase the milk shelf-life.
A COVID-19 Overview and Potential Applications of Cell Therapy
Authors: Câmara Diana Aparecida Dias , Porcacchia Allan Saj , Lizier Nelson Foresto , De-Sá-Júnior Paulo Luiz
Published: August 2021

The COVID-19 pandemic has already reaped thousands of lives, although many scientific studies already showed the possibility of this scenario. Currently, further attention is provided to patients depicting comorbidities such as respiratory or immunocompromised diseases, hypertension, and diabetes, as these individuals show a worse prognosis. Cell therapies using stem cells and/or defense cells, combined or not with traditional treatment, could be an outstanding strategy for COVID-19 management since these treatments can act by modulating the immune system, reducing proliferation, and favoring the complete elimination of the virus. In this review, we highlight the main molecular characteristics of this novel coronavirus, as well as the main pathognomonic signs of COVID-19. Furthermore, possible cell therapies are pointed out to show alternative treatments against COVID-19 and its sequels.
Vitamin C Enhances Antiviral Functions of Lung Epithelial Cells
Authors: Teafatiller Trevor , Agrawal Sudhanshu , De Robles Gabriela , Rahmatpanah Farah , Subramanian Veedamali S. , Agrawal Anshu
Published: August 2021

Vitamin C is well documented to have antiviral functions; however, there is limited information about its effect on airway epithelial cells—the first cells to encounter infections. Here, we examined the effect of vitamin C on human bronchial epithelium transformed with Ad12-SV40 2B (BEAS-2B) cells, and observed that sodium-dependent vitamin C transporter 2 (SVCT2) was the primary vitamin C transporter. Transcriptomic analysis revealed that treating BEAS-2B cells with vitamin C led to a significant upregulation of several metabolic pathways and interferon-stimulated genes (ISGs) along with a downregulation of pathways involved in lung injury and inflammation. Remarkably, vitamin C also enhanced the expression of the viral-sensing receptors retinoic acid-inducible gene 1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA-5), which was confirmed at the protein and functional levels. In addition, the lungs of l-gulono-γ-lactone oxidase knockout (GULO-KO) mice also displayed a marked decrease in these genes compared to wild-type controls. Collectively, our findings indicate that vitamin C acts at multiple levels to exert its antiviral and protective functions in the lungs.
Nanogel-Facilitated In-Situ Delivery of a Cataract Inhibitor
Authors: Gautam Dixa , Pedler Michelle G. , Nair Devatha P. , Petrash Jonathan Mark
Published: August 2021

Cataracts are a leading cause of blindness worldwide. Surgical removal of cataracts is a safe and effective procedure to restore vision. However, a large number of patients later develop vision loss due to regrowth of lens cells and subsequent degradation of the visual axis leading to visual disability. This postsurgical complication, known as posterior capsular opacification (PCO), occurs in up to 30% of cataract patients and has no clinically proven pharmacological means of prevention. Despite the availability of many compounds capable of preventing early steps in PCO development, there is currently no effective means to deliver such therapies into the eye for a suitable duration. To model a solution to this unmet medical need, we fabricated acrylic substrates as intraocular lens (IOL) mimics scaled to place into the capsular bag of the mouse lens following a mock-cataract surgery. Substrates were coated with a hydrophilic crosslinked acrylate nanogel designed to elute Sorbinil, an aldose reductase inhibitor previously shown to suppress PCO. Insertion of the Sorbinil-eluting device into the lens capsule at the time of cataract surgery resulted in substantial prevention of cellular changes associated with PCO development. This model demonstrates that a cataract inhibitor can be delivered into the postsurgical lens capsule at therapeutic levels.
A Novel Splice Variant of the Inhibitor of Growth 3 Lacks the Plant Homeodomain and Regulates Epithelial–Mesenchymal Transition in Prostate Cancer Cells
Authors: Melekhova Anna , Leeder Mirjam , Pungsrinont Thanakorn , Schmäche Tim , Kallenbach Julia , Ehsani Marzieh , Mirzakhani Kimia , Rasa Seyed Mohammad Mahdi , Neri Francesco , Baniahmad Aria
Published: August 2021

Inhibitor of growth 3 (ING3) is one of five members of the ING tumour suppressor family, characterized by a highly conserved plant homeodomain (PHD) as a reader of the histone mark H3K4me3. ING3 was reported to act as a tumour suppressor in many different cancer types to regulate apoptosis. On the other hand, ING3 levels positively correlate with poor survival prognosis of prostate cancer (PCa) patients. In PCa cells, ING3 acts rather as an androgen receptor (AR) co-activator and harbours oncogenic properties in PCa. Here, we show the identification of a novel ING3 splice variant in both the human PCa cell line LNCaP and in human PCa patient specimen. The novel ING3 splice variant lacks exon 11, ING3∆ex11, which results in deletion of the PHD, providing a unique opportunity to analyse functionally the PHD of ING3 by a natural splice variant. Functionally, overexpression of ING3Δex11 induced morphological changes of LNCaP-derived 3D spheroids with generation of lumen and pore-like structures within spheroids. Since these structures are an indicator of epithelial–mesenchymal transition (EMT), key regulatory factors and markers for EMT were analysed. The data suggest that in contrast to ING3, ING3Δex11 specifically modulates the expression of key EMT-regulating upstream transcription factors and induces the expression of EMT markers, indicating that the PHD of ING3 inhibits EMT. In line with this, ING3 knockdown also induced the expression of EMT markers, confirming the impact of ING3 on EMT regulation. Further, ING3 knockdown induced cellular senescence via a pathway leading to cell cycle arrest, indicating an oncogenic role for ING3 in PCa. Thus, the data suggest that the ING3Δex11 splice variant lacking functional PHD exhibits oncogenic characteristics through triggering EMT in PCa cells.