bluuebunny/crossref_metadata_2025 huggingface dataset:
Fertility Sparing Treatment in Gastric-Type Endocervical Carcinoma
Authors: Rychlik Agnieszka , Querleu Denis , Bidzinski Mariusz
Published: October 2021

Fertility sparing management of cancer is one of the main components of quality-of-life issues. Early-stage cervical cancer, frequently diagnosed in women of reproductive age, can potentially be treated conservatively. However, some rare histological types of cervical cancer present with aggressive clinical behavior. Particularly, in the newly introduced concept of gastric-type endocervical carcinoma, fertility sparing management is ‘a priori’ not recommended. Even so, this issue remains undocumented. For this reason, a selected review of the current literature on gastric type endocervical carcinoma was carried out through PubMed. The keywords included “gastric-type cervical cancer”, “gastric-type endocervical carcinoma”, “conservative surgery”, “conservative treatment”, “fertility sparing surgery”, “radical trachelectomy”, “laparoscopic trachelectomy”, “robotic trachelectomy”, “laparotomic trachelectomy”, “abdominal trachelectomy”, “trachelectomy”, “neoadjuvant chemotherapy”, “conisation”, and “cone resection”. A search in the European Network on Cancer, Infertility and Pregnancy (INCIP) database was performed. The rarity of gastric-type endocervical carcinoma does not allow for conclusions on fertility sparing management with solid evidence. However, diffuse character of the disease and aggressive clinical behavior contraindicate a conservative treatment in young women with gastric type cervical cancer.
Tuning the Electronic Structure of CoO Nanowire Arrays by N-Doping for Efficient Hydrogen Evolution in Alkaline Solutions
Authors: Cao Maoqi , Li Xiaofeng , Xiang Dingding , Wu Dawang , Sun Sailan , Dai Hongjing , Luo Jun , Zou Hongtao
Published: October 2021

Electrochemical hydrogen evolution reactions (HER) have drawn tremendous interest for the scalable and sustainable conversion of renewable electricity to clear hydrogen fuel. However, the sluggish kinetics of the water dissociation step severely restricts the high production of hydrogen in alkaline media. Tuning the electronic structure by doping is an effective method to boost water dissociation in alkaline solutions. In this study, N-doped CoO nanowire arrays (N-CoO) were designed and prepared using a simple method. X-ray diffraction (XRD), element mappings and X-ray photoelectron spectroscopy (XPS) demonstrated that N was successfully incorporated into the lattice of CoO. The XPS of Co 2p and O 1s suggested that the electronic structure of CoO was obviously modulated after the incorporation of N, which improved the adsorption and activation of water molecules. The energy barriers obtained from the Arrhenius relationship of the current density at different temperatures indicated that the N-CoO nanowire arrays accelerated the water dissociation in the HER process. As a result, the N-CoO nanowire arrays showed an excellent performance of HER in alkaline condition. At a current density of 10 mA cm−1, the N-CoO nanowire arrays needed only a 123 mV potential, which was much lower than that of CoO (285 mV). This simple design strategy provides some new inspiration to promote water dissociation for HER in alkaline solutions at the atomic level.
Quantifying Renin-Angiotensin-System Alterations in COVID-19
Authors: Pucci Fabrizio , Annoni Filippo , dos Santos Robson Augusto Souza , Taccone Fabio Silvio , Rooman Marianne
Published: October 2021

The renin-angiotensin system (RAS) plays a pivotal role in a wide series of physiological processes, among which inflammation and blood pressure regulation. One of its key components, the angiotensin-converting enzyme 2, has been identified as the entry point of the SARS-CoV-2 virus into the host cells, and therefore a lot of research has been devoted to study RAS dysregulation in COVID-19. Here we discuss the alterations of the regulatory RAS axes due to SARS-CoV-2 infection on the basis of a series of recent clinical investigations and experimental analyzes quantifying, e.g., the levels and activity of RAS components. We performed a comprehensive meta-analysis of these data in view of disentangling the links between the impaired RAS functioning and the pathophysiological characteristics of COVID-19. We also review the effects of several RAS-targeting drugs and how they could potentially help restore the normal RAS functionality and minimize the COVID-19 severity. Finally, we discuss the conflicting evidence found in the literature and the open questions on RAS dysregulation in SARS-CoV-2 infection whose resolution would improve our understanding of COVID-19.
Cellular and Molecular Mechanisms Underlying Liver Fibrosis Regression
Authors: Caligiuri Alessandra , Gentilini Alessandra , Pastore Mirella , Gitto Stefano , Marra Fabio
Published: October 2021

Chronic liver injury of different etiologies may result in hepatic fibrosis, a scar formation process consisting in altered deposition of extracellular matrix. Progression of fibrosis can lead to impaired liver architecture and function, resulting in cirrhosis and organ failure. Although fibrosis was previous thought to be an irreversible process, recent evidence convincingly demonstrated resolution of fibrosis in different organs when the cause of injury is removed. In the liver, due to its high regenerative ability, the extent of fibrosis regression and reversion to normal architecture is higher than in other tissues, even in advanced disease. The mechanisms of liver fibrosis resolution can be recapitulated in the following main points: removal of injurious factors causing chronic hepatic damage, elimination, or inactivation of myofibroblasts (through various cell fates, including apoptosis, senescence, and reprogramming), inactivation of inflammatory response and induction of anti-inflammatory/restorative pathways, and degradation of extracellular matrix. In this review, we will discuss the major cellular and molecular mechanisms underlying the regression of fibrosis/cirrhosis and the potential therapeutic approaches aimed at reversing the fibrogenic process.
Cochlear Inflammaging in Relation to Ion Channels and Mitochondrial Functions
Authors: Bazard Parveen , Pineros Jennifer , Frisina Robert D. , Bauer Mark A. , Acosta Alejandro A. , Paganella Lauren R. , Borakiewicz Dominika , Thivierge Mark , Mannering Freyda L. , Zhu Xiaoxia , Ding Bo
Published: October 2021

The slow accumulation of inflammatory biomarker levels in the body—also known as inflammaging—has been linked to a myriad of age-related diseases. Some of these include neurodegenerative conditions such as Parkinson’s disease, obesity, type II diabetes, cardiovascular disease, and many others. Though a direct correlation has not been established, research connecting age-related hearing loss (ARHL)—the number one communication disorder and one of the most prevalent neurodegenerative diseases of our aged population—and inflammaging has gained interest. Research, thus far, has found that inflammatory markers, such as IL-6 and white blood cells, are associated with ARHL in humans and animals. Moreover, studies investigating ion channels and mitochondrial involvement have shown promising relationships between their functions and inflammaging in the cochlea. In this review, we summarize key findings in inflammaging within the auditory system, the involvement of ion channels and mitochondrial functions, and lastly discuss potential treatment options focusing on controlling inflammation as we age.
Electrical Ventricular Remodeling in Dilated Cardiomyopathy
Authors: Mages Christine , Gampp Heike , Syren Pascal , Rahm Ann-Kathrin , André Florian , Frey Norbert , Lugenbiel Patrick , Thomas Dierk
Published: October 2021

Ventricular arrhythmias contribute significantly to morbidity and mortality in patients with heart failure (HF). Pathomechanisms underlying arrhythmogenicity in patients with structural heart disease and impaired cardiac function include myocardial fibrosis and the remodeling of ion channels, affecting electrophysiologic properties of ventricular cardiomyocytes. The dysregulation of ion channel expression has been associated with cardiomyopathy and with the development of arrhythmias. However, the underlying molecular signaling pathways are increasingly recognized. This review summarizes clinical and cellular electrophysiologic characteristics observed in dilated cardiomyopathy (DCM) with ionic and structural alterations at the ventricular level. Furthermore, potential translational strategies and therapeutic options are highlighted.
Sensing Methods for Hazardous Phenolic Compounds Based on Graphene and Conducting Polymers-Based Materials
Authors: Hashim Hazwani Suhaila , Fen Yap Wing , Omar Nur Alia Sheh , Fauzi Nurul Illya Muhamad
Published: October 2021

It has been known for years that the phenolic compounds are able to exert harmful effects toward living organisms including humans due to their high toxicity. Living organisms were exposed to these phenolic compounds as they were released into the environment as waste products from several fast-growing industries. In this regard, tremendous efforts have been made by researchers to develop sensing methods for the detection of these phenolic compounds. Graphene and conducting polymers-based materials have arisen as a high potential sensing layer to improve the performance of the developed sensors. Henceforth, this paper reviews the existing investigations on graphene and conducting polymer-based materials incorporated with various sensors that aimed to detect hazardous phenolic compounds, i.e., phenol, 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, pentachlorophenol, 2-nitrophenol, 4-nitrophenol, 2,4-dinitrophenol, and 2,4-dimethylphenol. The whole picture and up-to-date information on the graphene and conducting polymers-based sensors are arranged in systematic chronological order to provide a clearer insight in this research area. The future perspectives of this study are also included, and the development of sensing methods for hazardous phenolic compounds using graphene and conducting polymers-based materials is expected to grow more in the future.
Off-Grid Electrical Cell Lysis Microfluidic Device Utilizing Thermoelectricity and Thermal Radiation
Authors: Duong Duong-Duy , Lee Nae-Yoon
Published: October 2021

Microfluidic devices have enormous potential and a wide range of applications. However, most applications end up as chip-in-a-lab systems because of power source constraints. This work focuses on reducing the reliance on the power network and expanding on the concept of a lab-on-a-chip for microfluidic devices. A cellulose-based radiator to reflect infrared (IR) radiation with wavelengths within the atmospheric window (8–13 µm) into outer space was fabricated. This process lowered the temperature inside the insulated environment. The difference in temperature was used to power a thermoelectric generator (TEG) and generate an electric current. This electric current was run through a DC-DC transformer to increase the voltage before being used to perform electrical cell lysis with a microfluidic device. This experimental setup successfully achieved 90% and 50% cell lysis efficiencies in ideal conditions and in field tests, respectively. This work demonstrated the possibility of utilizing the unique characteristics of a microfluidic device to perform an energy-intensive assay with minimal energy generated from a TEG and no initial power input for the system. The TEG system also required less maintenance than solar, wind, or hydroelectricity. The IR radiation process naturally allows for more dynamic working conditions for the entire system.
A Parameter Refinement Method for Ptychography Based on Deep Learning Concepts
Authors: Guzzi Francesco , Kourousias George , Gianoncelli Alessandra , Billè Fulvio , Carrato Sergio
Published: October 2021

X-ray ptychography is an advanced computational microscopy technique, which is delivering exceptionally detailed quantitative imaging of biological and nanotechnology specimens, which can be used for high-precision X-ray measurements. However, coarse parametrisation in propagation distance, position errors and partial coherence frequently threaten the experimental viability. In this work, we formally introduce these actors, solving the whole reconstruction as an optimisation problem. A modern deep learning framework was used to autonomously correct the setup incoherences, thus improving the quality of a ptychography reconstruction. Automatic procedures are indeed crucial to reduce the time for a reliable analysis, which has a significant impact on all the fields that use this kind of microscopy. We implemented our algorithm in our software framework, SciComPty, releasing it as open-source. We tested our system on both synthetic datasets, as well as on real data acquired at the TwinMic beamline of the Elettra synchrotron facility.
Producing Iron Endohedral Fullerene on Electron Cyclotron Resonance Ion Source
Authors: Kato Yushi , Omori Takayuki , Owada Issei , Kubo Wataru , Harisaki Shuhei , Sato Koichi , Tsuda Kazuki , Maenaka Takumu , Anan Masahiro , Muramatsu Masayuki , Kitagawa Atsushi , Yoshida Yoshikazu
Published: October 2021

An electron cyclotron resonance (ECR) ion source (ECRIS) can generate an available amount of multicharged ions, thus it is not limited for use in the field of accelerator science, but also in medical/biological fields, such as for heavy ion beam cancer treatment and ion engines. The processes of generating multicharged ions are mainly sequential collisions of a direct ionization process by electrons, and have good ion confinement characteristics. By utilizing this confinement property, we have synthesized iron-encapsulated fullerenes, which are supramolecular and can be expected to have various high functions. Fullerenes and iron ions are vaporized from pure solid materials and introduced into the ECRIS together with the support gas. We investigated conditions under which fullerene ions do not dissociate and iron ions are generated so that both can coexist. Generated ions are extracted from the ECRIS and separated by mass/charge with a dipole magnet, and detected with a Faraday cup. This measurement system is characterized by a wide dynamic range. The charge-state distribution (CSD) of ion currents was measured to investigate the optimum conditions for supramolecular synthesis. As a result, a significant spectrum suggesting the possibility of iron-encapsulated fullerenes was obtained. This paper describes the details of these experimental results.
Crystalline Phase Transitions and Reactivity of Ammonium Nitrate in Systems Containing Selected Carbonate Salts
Authors: Kaniewski Maciej , Huculak-Mączka Marta , Zieliński Jakub , Biegun Marcin , Hoffmann Krystyna , Hoffmann Józef
Published: October 2021

Samples of pure ammonium nitrate (AN) and its mixtures with calcium carbonate, potassium hydrogen carbonate and potassium carbonate were investigated with the use of differential thermal analysis with mass spectrometry, powder X-ray diffraction and scanning electron microscopy. The main objective of the study was to determine the influence of selected carbonate materials on phase transitions of ammonium nitrate and to consider a possibility to use such potassium salts as fillers in fertilizer production. It was proven that all carbonate salts caused the absence of a phase transition that normally would occur at around 84–86 °C. Potassium carbonates were too reactive in systems containing AN. Based on the performed study, it was concluded that even though potassium carbonates are not fit to replace mineral fillers in the production process of fertilizers containing ammonium nitrate, they could be used in lesser amounts to remove the presence of low-temperature phase transitions of AN.
Relationship between Structural Stiffness and Viscous Damping Coefficient in Reinforced Carbon Structure under Varying Carbon Fiber Angles
Authors: Kim Chan-Jung
Published: October 2021

A linearized dynamic model of a carbon-fiber-reinforced plastic (CFRP) structure can be formulated using the structural stiffness and viscous damping coefficient. The carbon fiber angle is an influential factor in determining the structural stiffness of CFRP structures by serially combining the stiffness of a binding matrix and that of a carbon fiber. The viscous damping coefficient of the CFRP structure is also highly sensitive to the carbon fiber angle; that is, it assumes a parallel series between the damping coefficient of the binding matrix and that of the carbon fiber. In this study, a sensitivity formula was derived to obtain the ratio of two parameters—the structural stiffness, and the viscous damping coefficient—by dividing all parameters by the value of the reference angle. The CFRP structure was chosen for a simple rectangular specimen with five carbon fiber angles, ranging from 0° (reference) to 90°. The identified modal parameters were used from the impact modal test conducted in a previous study. Sensitivity analysis was conducted for both the structural stiffness and the viscous damping coefficient. The sensitivity results revealed that the sensitivity index of the viscous damping coefficient was proportional to that of the structural stiffness. Even a small value of the viscous damping coefficient of the carbon fiber was sensitive to the CFRP structure because the carbon-fiber damping coefficient was parallel to the large damping coefficient of the binding matrix.
Exploring Performances of Electric Micro-Mobility Vehicles and Behavioural Patterns of Riders for In-Depth Accident Analysis
Authors: Gulino Michelangelo-Santo , Zonfrillo Giovanni , Damaziak Krzysztof , Vangi Dario
Published: October 2021

(1) Background: Electric micro-mobility vehicles (i.e., e-bikes and e-scooters) represent a fast-growing portion of the circulating fleet, leading to a multiplication of accident cases also attributable to risky behaviours adopted by the riders. Still, data on vehicle performance and rider behaviour are sparse and difficult to interpret (if not unavailable). Information regarding the overall accident dynamics can, however, aid in identifying users’ risky riding behaviour that actually led to a harmful event, allowing one to propose efficient strategies and policies to reduce the occurrence of road criticalities. (2) Methods: Speed and acceleration data of six cyclists of traditional and electric bikes were extracted from six closed-circuit experiments and real road tests performed in the city of Florence (Italy) to derive their behavioural patterns in diverse road contexts. (3) Results: The application of analysis of variance and linear regression procedures to such data highlights differences between men and women in terms of performance/behaviour in standing start; additionally, the use of e-bikes favours a higher speed ride in correspondence to roundabouts and roads with/without the right of way. To thoroughly assess the rider’s responsibilities in an eventual accident, an ancillary procedure was highlighted to evaluate whether a micro-mobility vehicle complies with the applicable regulations. (4) Conclusion: With these results, the prospective recognition of rider behaviour was facilitated during the investigation process, and the abilities to extract such relevant information from in-depth accident data wereconsequently enhanced.
STAT3 Activation in Psoriasis and Cancers
Authors: Kishimoto Megumi , Komine Mayumi , Sashikawa-Kimura Miho , Ansary Tuba Musarrat , Kamiya Koji , Sugai Junichi , Mieno Makiko , Kawata Hirotoshi , Sekimoto Ryutaro , Fukushima Noriyoshi , Ohtsuki Mamitaro
Published: October 2021

Activation of signal transducer and activator of transcription (STAT)3 has been reported in many cancers. It is also well known that STAT3 is activated in skin lesions of psoriasis, a chronic skin disease. In this study, to ascertain whether patients with psoriasis have a predisposition to STAT3 activation, we examined phosphorylated STAT3 in cancer cells of psoriasis patients via immunohistochemistry. We selected patients with psoriasis who visited the Department of Dermatology, Jichi Medical University Hospital, from January 2000 to May 2015, and had a history of cancer. We performed immunostaining for phosphorylated STAT3 in tumor cells of five, four, and six cases of gastric, lung, and head and neck cancer, respectively. The results showed that there was no significant difference in STAT3 activation in any of the three cancer types between the psoriasis and control groups. Although this study presents limitations in its sample size and inconsistency in the histology and differentiation of the cancers, results suggest that psoriasis patients do not have a predisposition to STAT3 activation. Instead, STAT3 activation is intricately regulated by each disorder or cellular microenvironment in both cancer and psoriasis.
Body Fat Plays an Important Role in of Bioimpedance Spectroscopy-Based Dry Weight Measurement Error for Patients with Hemodialysis
Authors: Kim Hae-Ri , Jeon Jae-Wan , Bae Hong-Jin , Shin Jin-Ah , Ham Young-Rok , Na Ki-Ryang , Lee Kang-Wook , Choi Dae-Eun , Hyon Yun-Kyong
Published: October 2021

Accurate dry weight (DW) estimation is important for hemodialysis patients. Although bioimpedance spectroscopy (BIS) is commonly used to measure DW, the BIS-based DW frequently differs from the clinical DW. We analyzed the characteristics of patients whose BIS-based DWs were over- and underestimated. In this retrospective cohort study, we evaluated 1555 patients undergoing maintenance hemodialysis in Chungnam National University Hospital. The gap (DWCP-BIS) was calculated by comparing the BIS and clinical DWs. We analyzed the clinical characteristics of patients with positive (n = 835) and negative (n = 720) gaps. Compared with other patients, the DWCP-BIS-positive group had higher extracellular water (ECW) level and extracellular/intracellular water index (E/I) and had lower weight, body mass index (BMI), lean tissue index (LTI), fat tissue index (FTI), fat mass (FAT), and adipose tissue mass (ATM). The DWCP-BIS-negative group exhibited elevated BMI, FTI, FAT, and ATM; however, it had lower height, ECW, and E/I. Linear regression analysis revealed that FAT significantly predicted DWCP accuracy. The clinical DW of patients with a low fat mass tended to be underestimated, while the clinical DW of patients with comparatively large fat reserves tended to be overestimated. These characteristics will aid in the reduction of BIS-based DW errors.
Patterns of Scientific Reasoning Skills among Pre-Service Science Teachers: A Latent Class Analysis
Authors: Khan Samia , Krell Moritz
Published: October 2021

We investigated the scientific reasoning competencies of pre-service science teachers (PSTs) using a multiple-choice assessment. This assessment targeted seven reasoning skills commonly associated with scientific investigation and scientific modeling. The sample consisted of 112 PSTs enrolled in a secondary teacher education program. A latent class (LC) analysis was conducted to evaluate if there are subgroups with distinct patterns of reasoning skills. The analysis revealed two subgroups, where LC1 (73% of the PSTs) had a statistically higher probability of solving reasoning tasks than LC2. Specific patterns of reasoning emerged within each subgroup. Within LC1, tasks involving analyzing data and drawing conclusions were answered correctly more often than tasks involving formulating research questions and generating hypotheses. Related to modeling, tasks on testing models were solved more often than those requiring judgment on the purpose of models. This study illustrates the benefits of applying person-centered statistical analyses, such as LC analysis, to identify subgroups with distinct patterns of scientific reasoning skills in a larger sample. The findings also suggest that highlighting specific skills in teacher education, such as: formulating research questions, generating hypotheses, and judging the purposes of models, would better enhance the full complement of PSTs’ scientific reasoning competencies.
Call Model and Test-Verification Methods for PS-LTE Core Equipment
Authors: Lee Jae-Jeong , Park Pyung-Koo , Chung Byung-Chang , Kim Sang-Wan , Nam Ki-Dong , Rhee Woo-Seop
Published: October 2021

Existing technologies in disaster and safety communication networks (DSCNs) face problems such as narrow bandwidth, low transmission rate, and difficulty in securing channels. Conversely, public safety long-term evolution (PS-LTE) technology provides high-speed, low-latency, quick-setup, and high-security data access for multimedia-based mission-critical services. To perform tests on each unit of core equipment with PS-LTE technology, a new call model such as push-to-talk, which is a service representing PS-LTE, is required. Moreover, objective and consistent metrics and methods are required for administering tests on the unique functions of DSCNs newly constructed with PS-LTE technology. This paper proposes a call model for the core facilities of DSCNs established in Korea, and it also suggests test and verification methods for all the areas of PS-LTE, including mission-critical push-to-talk, evolved multimedia broadcast and multicast service, and coverage and quality assessment for wireless services.
Performance Analysis of PI and DMRAC Algorithm in Buck–Boost Converter for Voltage Tracking in Electric Vehicle Using Simulation
Authors: Islam Maidul , Abdul Ghaffar Alia Farhana , Sulaeman Erwin , Ahsan Md Manjurul , Kouzani Abbas Z. , Mahmud M. A. Parvez
Published: October 2021

This study introduces a Direct Model Reference Adaptive Control (DMRAC) algorithm in a buck–boost converter in the power distribution of an electric vehicle. In this study, DMRAC was used in order to overcome the system nonlinearity due to load demand variation, in case of different driving modes (such as acceleration, stable and regenerative braking system mode), and the presence of disturbances in the system. DMRAC receives popularity because of its robustness in the presence of nonlinearity and ensuring system stability. To evaluate the efficacy of DMRAC in the current system, its performance was compared with a PI controller in the MATLAB/Simulink environment. The simulation results show the superiority of DMRAC over a conventional PI control approach, in both variable load demand and disturbed system cases that were measured by tracking error. The improvement was seen in the DMRAC response, with smaller tracking error and faster transient and disturbance rejection. The main contribution of this work is in introducing DMRAC, particularly in a buck–boost converter, and its efficacy with a DC–DC converter for an electric vehicle, which has not been studied before.
Recreation and Therapy in Urban Forests—The Potential Use of Sensory Garden Solutions
Authors: Wajchman-Świtalska Sandra , Zajadacz Alina , Lubarska Anna
Published: October 2021

Urban forests are not only woodlands or groups of trees, but also individual trees, street trees, trees in parks, trees in derelict corners, and gardens. All of which are located in urban and peri-urban areas and diversify the landscape and provide a wide range of social benefits. Sensory gardens play a specific therapeutic and preventive role. Designing such gardens as a recreational infrastructure element can successfully enrich urban forests. Following the principles of universal design may provide enjoyment for all city-dwellers, with special attention given to the needs of individuals with disabilities. We studied 15 gardens and one sensory path located in various regions in Poland. The inventory was carried out on the basis of the features considered important in spatial orientation by blind and partially sighted people. The results showed that the solutions used were only partly adequate for the needs of selected users. We found neither tactile walking surface indicators (e.g., communication lines and terrain), spatial models, nor applications in mobile devices. However, these could be useful for all visitors. We confirmed that although problems with the use of forest tourist space are dependent on the type of disability, by implementing the idea of universal design for all elements of recreational infrastructure, forests may be accessible for all users.
Melatonin in Plant Defense against Abiotic Stress
Authors: Rehaman Abdul , Mishra Awdhesh Kumar , Ferdose Asma , Per Tasir S. , Hanief Mohd , Jan Arif Tasleem , Asgher Mohd
Published: October 2021

Abiotic stress adversely affects plant growth and metabolism and as such reduces plant productivity. Recognized as a major contributor in the production of reactive oxygen species (ROS), it hinders the growth of plants through induction of oxidative stress. Biostimulants such as melatonin have a multifunctional role, acting as a defense strategy in minimizing the effects of oxidative stress. Melatonin plays important role in plant processes ranging from seed germination to senescence, besides performing the function of a biostimulant in improving the plant’s productivity. In addition to its important role in the signaling cascade, melatonin acts as an antioxidant that helps in scavenging ROS, generated as part of different stresses among plants. The current study was undertaken to elaborate the synthesis and regulation of melatonin in plants, besides emphasizing its function under various abiotic stress namely, salt, temperature, herbicides, heavy metals, and drought. Additionally, a special consideration was put on the crosstalk of melatonin with phytohormones to overcome plant abiotic stress.
Profile of Producers and Production of Dry-Aged Beef in Brazil
Authors: Rezende-de-Souza Jonatã Henrique , Cardello Flavio Andre Bolini , de Paula Ana Paula Moraes , Ribeiro Felipe A. , Calkins Chris R. , Pflanzer Sérgio Bertelli
Published: October 2021

No information is currently available on the profile of producers and production process of dry-aged beef in Brazil, to the best of the authors’ knowledge. We surveyed 37 Brazilian companies that were producing dry-aged beef in 2020 to investigate this market. The absolute and relative frequency of responses was calculated to obtain the sum, average, minimum, and maximum values. From the respondents, dry-aged beef was first produced in 2009, and most producers are located in big cities. Most respondents control and monitor chamber temperature; however, humidity and air velocity only are monitored. The aging period (mostly between 22 to 60 days) was the main indicator of product readiness. The process losses (water loss and crust trimming) can reach 65%. Some producers perform microbiological analyses to ensure product safety and others use tools such as GMP and SOP. The results of this survey may help governmental institutions to develop a standardized industrial protocol for producing dry-aged beef in Brazil.
Global Proteomic Analysis of Listeria monocytogenes’ Response to Linalool
Authors: Gao Zhipeng , Zhong Weiming , Liu Ting , Zhao Tianyu , Guo Jiajing
Published: October 2021

Listeria monocytogenes (LM) is one of the most serious foodborne pathogens. Listeriosis, the disease caused by LM infection, has drawn attention worldwide because of its high hospitalization and mortality rates. Linalool is a vital constituent found in many essential oils; our previous studies have proved that linalool exhibits strong anti-Listeria activity. In this study, iTRAQ-based quantitative proteomics analysis was performed to explore the response of LM exposed to linalool, and to unravel the mode of action and drug targets of linalool against LM. A total of 445 differentially expressed proteins (DEPs) were screened out, including 211 up-regulated and 234 down-regulated proteins which participated in different biological functions and pathways. Thirty-one significantly enriched gene ontology (GO) functional categories were obtained, including 12 categories in “Biological Process”, 10 categories in “Cell Component”, and 9 categories in “Molecular Function”. Sixty significantly enriched biological pathways were classified, including 6 pathways in “Cell Process”, 6 pathways in “Environmental Information Processing”, 3 pathways in “Human Disease”, 40 pathways in “Metabolism”, and 2 pathways in “Organic System”. GO and Kyoto Encyclopedia of Genes (KEGG) enrichment analysis together with flow cytometry data implied that cell membranes, cell walls, nucleoids, and ribosomes might be the targets of linalool against LM. Our study provides good evidence for the proteomic analysis of bacteria, especially LM, exposed to antibacterial agents. Further, those drug targets discovered by proteomic analysis can provide theoretical support for the development of new drugs against LM.
Anti-Helicobacter pylori Activity of a Lactobacillus sp. PW-7 Exopolysaccharide
Authors: Hu Jingfei , Tian Xueqing , Wei Tong , Wu Hangjie , Lu Jing , Lyu Mingsheng , Wang Shujun
Published: October 2021

Helicobacter pylori is a cause of gastric cancer. We extracted the exopolysaccharide (EPS) of Lactobacillus plajomi PW-7 for antibacterial activity versus H. pylori, elucidating its biological activity and structural characteristics. The minimum inhibitory concentration (MIC) of EPS against H. pylori was 50 mg/mL. Disruption of the cell membranes of pathogenic bacteria by EPS was indicated via the antibacterial mechanism test and confirmed through electron microscopy. EPS also has antioxidant capacity. The IC50 of EPS for 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radicals were 300 μg/mL, 180 μg/mL, and 10 mg/mL, respectively. The reducing power of EPS was 2 mg/mL, equivalent to 20 μg/mL of ascorbic acid. EPS is a heteropolysaccharide comprising six monosaccharides, with an approximate molecular weight of 2.33 × 104 Da. Xylose had a significant effect on H. pylori. EPS from L. plajomi PW-7 showed potential as an antibacterial compound and antioxidant, laying a foundation for the development of EPS-based foods.
Comparative Fatty Acid Profiling of Edible Fishes in Kuala Terengganu, Malaysia
Authors: Tramice Annabella , Trifuoggi Marco , Ahmad Mohammad Fadhli , Lam Su Shiung , Iodice Carmine , Velotto Gennaro , Giarra Antonella , Inglese Sara , Cupo Adelaide , Guerriero Giulia , Tommonaro Giuseppina
Published: October 2021

The aim of this study was to compare the relative nutritional benefit of edible Malaysian fishes from the coast of Terengganu in Malaysia, as well as to perform a taxonomical characterization and metal assessment. Discrimination between species was carried out by a morphological and molecular approach by evaluating the total concentrations of metals by ICP-MS analyses and the fatty acids (FA) composition using the GC–MS approach on the fish fillet tissues. The taxonomical studies detected fishes of 11 families and 13 species. The heavy metal assessment showed that all detected elements did not exceed the regulatory limit stated by Malaysian Food Regulations. The proportion of saturated fatty acids (SFA) ranged from 33 to 58.34%, followed by the polyunsaturated fatty acids (PUFA) values from 24 to 51.8%, and the lowest proportion was of monounsaturated fatty acids (MUFA), ranging from 12.7 to 35.9%. The ω-3/ω-6 PUFA and PUFA/SFA ratios were determined in the range 1.1 to 7.4 and 0.35 to 1.6, respectively. The C20:5 ω-3 and C22:6 ω-3 acids were detected at levels comparable to those found in the corresponding species from similar tropical marine ecosystems. The high FA values can be useful biochemical tools for comparing the relative nutritional benefits of these biodiverse and non-toxic edible Malaysian fishes.
Effects of Tartary Buckwheat Protein on Gut Microbiome and Plasma Metabolite in Rats with High-Fat Diet
Authors: Liu Jing , Song Yu , Zhao Qi , Wang Yuguo , Li Congshou , Zou Liang , Hu Yichen
Published: October 2021

The prevalence of lipid metabolism diseases, mainly obesity, fatty liver, and hyperlipidemia, is increasing in the world. Tartary buckwheat is a kind of medicinal and edible crop, and clinical experiments have also confirmed that dietary Tartary buckwheat can effectively regulate lipid metabolism disorders. Tartary buckwheat protein (TBP), as the main active ingredient of Tartary buckwheat, has an effect of blood lipid reduction that has been widely reported. In this paper, we investigated the constituents of TBP and then evaluated the hypolipidemic effect of TBP in hyperlipidemia rats. Male Sprague–Dawley rats were fed a high-fat diet for six weeks to induce hyperlipidemia and then given TBP orally for five weeks. The effects of TBP on body weight, serum lipids, liver lipids, liver oxidative stress, pathological organization, gut microbiota, and plasma metabolites were analyzed. At the serum level, TBP supplement significantly decrease the level of LDL-C and increase the level of HDL-C. At the liver level, it can reduce the levels of TC, TG, and LDL-C. The potential mechanism of action is, on the one hand, to increase the abundance of the Lachnospiraceae and the Ruminococcaceae by modulating the gut microbiota, facilitating the productivity of short-chain fatty acids, and increasing fecal bile acid excretion and, on the other hand, may be related to the improvement of bile acid metabolism.
Structural and Emulsifying Properties of Citric Acid Extracted Satsuma Mandarin Peel Pectin
Authors: Duan Xingke , Yang Zhixuan , Yang Jinyan , Liu Fengxia , Xu Xiaoyun , Pan Siyi
Published: October 2021

Satsuma mandarin peel pectin (MPP) was extracted by citric acid and its structure and emulsifying ability were evaluated. Structural characterization, including NMR, FTIR, monosaccharide compositions demonstrated that MMP showed lower DM value and higher Mw than commercial citrus pectin (CCP). In addition, MPP exhibited significantly better emulsification performance than CCP. When MPP concentration was increased to 1%, 1.5% (10 g/L, 15 g/L) and the pH was 3 (acidic condition), a stable emulsion containing 10% oil fraction could be obtained. The particle size of the obtained emulsion was ranging from 1.0–2.3 μm, its emulsifying activity ranged from 93–100% and emulsifying stability was 94–100%. Besides, MPP can better ensure the storage stability of higher oil ratio emulsions. The results demonstrated that the stable emulsifying properties of MPP may largely depend on the lower DM value and higher Mw. MPP could be used as a novel polysaccharide emulsifier, especially under acidic conditions, providing a promising alternative for natural emulsifiers that could be used in the food industry.
Effects of Enzymatic Hydrolysis on Physicochemical Properties and Solubility and Bitterness of Milk Protein Hydrolysates
Authors: Cui Qiang , Sun Yuxue , Zhou Zengjia , Cheng Jianjun , Guo Mingruo
Published: October 2021

Milk protein concentrate (MPC) is a high-protein dairy product. It is underutilized due to its poor solubility compared with other milk protein products. This study aimed to investigate the effect of enzymatic hydrolysis on the physicochemical properties and solubility of MPC. Results showed that Alcalase hydrolysates possessed a higher degree of hydrolysis (DH) than Protamex and Flavourzyme hydrolysates. Similar results could be obtained using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of the hydrolysate of Alcalase was less than 10 kDa. Changes to the molecular weight thereby led to a modification in the fluorescence intensity, Fourier-transform infrared spectrometry, and ultraviolet absorption. The solubility of all hydrolysates was significantly increased (p < 0.05). Correlation analysis showed a positive correlation between solubility, DH, and bitterness; the correlation coefficients were 0.81 for DH and 0.61 for bitterness. Electronic tongue analysis showed that the bitterness of Alcalase hydrolysates was the highest, while the values for Protamex hydrolysates were the lowest.
An Integrated Bile Acids Profile Determination by UHPLC-MS/MS to Identify the Effect of Bile Acids Supplement in High Plant Protein Diet on Common Carp (Cyprinus carpio)
Authors: Wei Xian , Yao Ting , Fall Fatou Ndoye , Xue Min , Liang Xiaofang , Wang Jie , Du Wenlong , Gu Xu
Published: October 2021

Bile acids (BAs) have considerable importance in the metabolism of glycolipid and cholesterol. The purpose of the present study is to clarify the effects of bile acids supplementary in a high plant protein diet for the common carp BA profiles and hepatopancreas and intestine health. An 11-week feeding trial was conducted with high plant protein diet (18% soybean meal and 18% cottonseed protein concentrated) (HP) and HP added 600 mg/kg BAs (HP+BAs) for common carp, and then, the UHPLC-MS/MS technology was used to analyze the BAs in the bile and plasma of two groups. HP could induce vacuolation of hepatocytes and accumulation of glycogen in the common carp, while these phenotypes were significantly improved in the HP+BAs group. In addition, the BA profile of the HP group and HP+BAs group are described in detail, for the common carp bile with treatment by exogenous BAs, TCA, CA, TβMCA, and TωMCA were the main components. Furthermore, in the HP+BAs group plasma, CDCA, CA, LCA, and GCDCA increased significantly; they could activate TGR5, and the activation of hepatopancreas TGR5 might regulate glucose metabolism to relieve hepatopancreas glycogen accumulation. This study proved that BAs supplemented to plant protein diet could relieve the common carp hepatopancreas glycogen accumulation by changing the BAs’ profile, thereby promoting its healthy growth, which has important guiding significance for the promotion of aquaculture development and makes an important contribution to expanding the strategic space of food security.
Component Identification of Phenolic Acids in Cell Suspension Cultures of Saussureainvolucrata and Its Mechanism of Anti-Hepatoma Revealed by TMT Quantitative Proteomics
Authors: Gao Junpeng , Wang Yi , Lyu Bo , Chen Jian , Chen Guang
Published: October 2021

Saussurea involucrata (S. involucrata) had been reported to have anti-hepatoma function. However, the mechanism is complex and unclear. To evaluate the anti-hepatoma mechanism of S. involucrata comprehensively and make a theoretical basis for the mechanical verification of later research, we carried out this work. In this study, the total phenolic acids from S. involucrata determined by a cell suspension culture (ESPI) was mainly composed of 4,5-dicaffeoylquinic acid, according to the LC-MS analysis. BALB/c nude female mice were injected with HepG2 cells to establish an animal model of liver tumor before being divided into a control group, a low-dose group, a middle-dose group, a high-dose group, and a DDP group. Subsequently, EPSI was used as the intervention drug for mice. Biochemical indicators and differences in protein expression determined by TMT quantitative proteomics were used to resolve the mechanism after the low- (100 mg/kg), middle- (200 mg/kg), and high-dose (400 mg/kg) interventions for 24 days. The results showed that EPSI can not only limit the growth of HepG2 cells in vitro, but also can inhibit liver tumors significantly with no toxicity at high doses in vivo. Proteomics analysis revealed that the upregulated differentially expressed proteins (DE proteins) in the high-dose group were over three times that in the control group. ESPI affected the pathways significantly associated with the protein metabolic process, metabolic process, catalytic activity, hydrolase activity, proteolysis, endopeptidase activity, serine-type endopeptidase activity, etc. The treatment group showed significant differences in the pathways associated with the renin-angiotensin system, hematopoietic cell lineage, etc. In conclusion, ESPI has a significant anti-hepatoma effect and the potential mechanism was revealed.
Effect of Different Processing Methods on Quality, Structure, Oxidative Properties and Water Distribution Properties of Fish Meat-Based Snacks
Authors: Nawaz Asad , Li Enpeng , Khalifa Ibrahim , Walayat Noman , Liu Jianhua , Irshad Sana , Zahra Anam , Ahmed Shakeel , Simirgiotis Mario Juan , Pateiro Mirian , Lorenzo José M.
Published: October 2021

Snack foods are consumed around to globe due to their high nutrition, taste and versatility; however, the effects of various processing methods on quality, structure and oxidative properties are scare in the literature. This study aims to evaluate the effect of various processing methods (frying, baking and microwave cooking) on quality, structure, pasting, water distribution and protein oxidative properties of fish meat-based snacks. The results showed that the frying method induced a significantly (p < 0.05) higher expansion than baking and microwave methods. Texture in terms of hardness was attributed to the rapid loss of water from muscle fiber, which resulted in compact structure and the increased hardness in microwave cooking, whereas in frying, due to excessive expansion, the hardness decreased. The pasting properties were significantly higher in baking, indicating the sufficient swelling of starch granules, while low in microwave suggest the rapid heating, which degraded the starch molecules and disruption of hydrogen bonds as well as glycosidic linkage and weakening of granules integrity. The water movement assessed by Low Field Nuclear Magnetic Resonance (LF-NMR) showed that frying had less tight and immobilized water, whereas microwave and baking had high amounts of tight and immobilized water, attributing to the proper starch-protein interaction within matrix, which was also evidenced by scanning electron microscopy (SEM) analysis. The protein oxidation was significantly (p < 0.05) higher in frying compared to baking and microwave cooking. The findings suggest the endorsement of baking and microwave cooking for a quality, safe and healthy snacks.
Roles of Glutathione in Mediating Abscisic Acid Signaling and Its Regulation of Seed Dormancy and Drought Tolerance
Authors: Koramutla Murali Krishna , Negi Manisha , Ayele Belay T.
Published: October 2021

Plant growth and development and interactions with the environment are regulated by phytohormones and other signaling molecules. During their evolution, plants have developed strategies for efficient signal perception and for the activation of signal transduction cascades to maintain proper growth and development, in particular under adverse environmental conditions. Abscisic acid (ABA) is one of the phytohormones known to regulate plant developmental events and tolerance to environmental stresses. The role of ABA is mediated by both its accumulated level, which is regulated by its biosynthesis and catabolism, and signaling, all of which are influenced by complex regulatory mechanisms. Under stress conditions, plants employ enzymatic and non-enzymatic antioxidant strategies to scavenge excess reactive oxygen species (ROS) and mitigate the negative effects of oxidative stress. Glutathione (GSH) is one of the main antioxidant molecules playing a critical role in plant survival under stress conditions through the detoxification of excess ROS, maintaining cellular redox homeostasis and regulating protein functions. GSH has recently emerged as an important signaling molecule regulating ABA signal transduction and associated developmental events, and response to stressors. This review highlights the current knowledge on the interplay between ABA and GSH in regulating seed dormancy, germination, stomatal closure and tolerance to drought.