bluuebunny/crossref_metadata_2025 huggingface dataset:
EFFECT OF THE PERCEIVED SOCIAL SUPPORT ON SCHOOL SUCCESS OF CHILDREN WITH CHRONIC DISEASE
Authors: YETİŞ KOCA Gülsüm , AYLAZ Rukuye
Published: October 2021

This study was carried out to assess the effect of the perceived social support by chronic diseased students on their school success. This cross-sectional and descriptive research was conducted with hospitalised chronic diseased children aged between 10-16 years. Data of the study was collected by using Perceived Social Support Scale. The research has been conducted with a questionnaire method. School life of children with diagnosed chronic disease is interrupted by; frequent hospitalizations, controls, treatment approaches and medicational side effects. The children who participated the survey were determined to fall behind in their lessons and had decreased school success when compared to peers. While these factors affect children’s school adaptation and success, social support has been shown have positive effects on these topics. While chronic diseases were detected to have negative effects on children's school success, perceived social support has been determined to have positive effects. Besides, children stated that existence of a school nurse would not only help in their treatment and make them feel safer at school but also would prevent absenteeism. Social support and existence of a school nurse are important factors in the school success of chronic diseased children.
PROCUREMENT OF ULAYAT LAND FOR SUBSIDED HOUSING DEVELOPMENT IN HARAU DISTRICT LIMA PULUH KOTA REGENCY
Authors: Deviandari Inggir , Warman Kurnia , Nurdin Zefrizal
Published: October 2021

Ulayat rights are the rights that owned by a legal alliance, where the citizens of the community have the right to control the land. The Regional Regulation of West Sumatra Province, Article 2 paragraph (1) Number 6 of 2008 concerning Communal Land and Its Utilization states that the main principle of customary land is permanent based on the Minangkabau traditional philosophy “jua ndak makan bali, gadai ndak makan sando” (shall not bargaining; may not be pawned). The provisions of the regulation state that ulayat land may not be traded and may not be pawned, its ownership status may not change, except for importunate situation and condition, namely maik tabujua dalam rumah (for the death of family member), gadih gadang ndak balaki (wedding), rumah gadang katirisan (misfortune), mambangkik Batang tarandam (efforts to enforce). Land acquisition for the construction of subsidized housing is not a land acquisition according to Law Number 2 of 2012 concerning Land Procurement for Public Interest. The formulation of the problems discussed are first, the process of acquiring communal land for the construction of subsidized housing in Harau District, Lima Puluh Kota Regency, secondly the land registration process after the acquisition of customary land occurs, thirdly the legal consequences of acquiring customary land used for the construction of subsidized housing in Harau District, Lima Regency. Dozens of Cities againts customary law communities. This study uses an empirical juridical approach with the aim of finding out whether the law in the book is in accordance with the law in action. The results of the research study indicate that the process of acquiring communal land for the construction of subsidized housing is carried out by buying and selling. The construction of subsidized housing is managed by a legal entity in the form of a Limited Liability Company. The status of land ownership is just as the user of the facility that called with Hak Guna Bangunan
FEATURES OF THE FORMATION OF THE LOWER-MIDDLE CAMBRIAN DEPOSITS IN CONNECTION WITH THE ASSESSMENT OF OIL AND GAS POTENTIAL IN THE TERRITORY OF THE NORTH-TUNGUSKA OGA
Authors: Moiseev Sergey A. , Fomin Andrei M. , Gubin Igor A.
Published: October 2021

The key features of the structure, lithology and sedimentation conditions of the Lower and Middle Cambrian of the North Tunguska OGO are considered. A seismogeological model of the section in the zone of its facies replacement was compiled. A description of two paleogeographic schemes of the Toyon and May centuries is given. The high prospects for the oil and gas content of this territory have been substantiated.
ISTOPIC COMPOSITION (H, O AND C) OF NATURAL WATERS OF THE NOVOSIBIRSK CITY AGLOMERATION
Authors: Novikov Dmitry A. , Pyrayev Aleksandr N. , Dultsev Fedor F. , Chernykh Anatoliy V. , Sukhorukova Anna F. , Derkachov Anton S. , Maksimova Anastasia A.
Published: October 2021

The paper presents the first data on the regional distribution of the isotopic composition of oxygen and hydrogen in waters, as well as the carbon of water-dissolved carbon dioxide in natural surface and underground infiltration waters of the Novosibirsk urban agglomeration. For the presented sample of samples, the vector of changes in the values of δD and δO in the studied region was obtained from -112 to -126 ‰ and from -14 to -16 ‰, respectively. The water-dissolved carbon dioxide of the studied waters has the expected biogenic genesis, the isotopic composition of carbon (δC from -14.0 to -7.0 ‰) indicates its participation in the processes of silicate-carbonate weathering.
NEW DATA ON HYDROGEOCHEMISTRY OF THE MINERAL WATERS OF THE CRIMEAN PENINSULA
Authors: Novikov Dmitry A. , Khvashchevskaya Albina A. , Sidorina Natalia G. , Kohanenko Anna A. , Chernykh Anatoliy V. , Maksimova Anastasia A. , Dultsev Fedor F.
Published: October 2021

Relevant data on the composition of mineral waters from 23 deposits and occurrences in the Crimean Peninsula are presented. The characteristic of 7 occurrences that have been previously unknown is presented. The studied waters are distinguished by broad-range variations in chemical composition, geochemical parameters of the medium, and the concentrations of biologically active components. Waters with the composition SO4-Cl-HCO3 Na, Cl Na, SO4-HCO3 Mg-Ca-Na, SO4-Cl Mg-Nam with TDS from ultra-fresh to brine level (0.4 - 202.9 g/dm3) are dominating. The waters relate to two large groups according to the geochemical parameters of the medium. The first group is characterized by reductive conditions with Eh from -330.2 to -22.3, pH from 7.3 to 9.1 and O2dissolved concentration 0.79 - 6.48 mg/dm3. The second group is characterized by oxidative Eh (+22.1 - +158.5), neutral and weakly alkaline pH (7,0 - 8,4), and O2dissolved concentrations from 2.73 to 6.92 mg/dm3. The studied waters exhibit a broad range of balneological properties, which allowed us to distinguish 13 types of mineral waters.
TOXIC ELEMENTS IN NATURAL WATERS OF THE SEVASTOPOL URBAN AGGLOMERATION
Authors: Novikov Dmitry A. , Chernykh Anatoliy V. , Khvashchevskaya Albina A. , Dultsev Fedor F. , Nichkova Larisa A. , Sigora Galina A. , Homenko Tamara Yu. , Yahin Timur A.
Published: October 2021

The article presents the first results of the distribution of a wide range of highly toxic elements in the natural waters of the Sevastopol urban agglomeration. It was found that all waters do not exceed the MPC for V, Cr, Cu, Zn, Mo, Cd, Pb, and Bi; in a number of objects, the excess for Fe, Mn, Co, Ni and Tl was found. Among the standardized chemical elements, there are high indicators of water quality in village Kolkhoznoye, as well as on almost the entire territory of the Heraclean Peninsula. The droughts of the last three years have had a huge impact on the quality of quality resources, leading to the concentration of almost all microcomponents not only in surface waters, but also in groundwater.
FORMATION CONDITIONS OF GROUNDWATER OF THE UPPER JURASSIC OF THE CENTRAL REGIONS OF THE ZAURAL MEGAMONOCLYSIS
Authors: Novikov Dmitry A. , Pyrayev Aleksandr N. , Dultsev Fedor F. , Chernykh Anatoliy V. , Bakustina Svetlana V. , Ulyanov Dmitry V.
Published: October 2021

The article presents the first results of complex isotope-hydrogeochemical studies of reservoir waters of the Upper Jurassic deposits of the central regions of the Zaural megamonoclysis. It was shown that most waters have a narrow distribution of oxygen and hydrogen isotopes (δD from -103.2 to -85.6 ‰ and δO from -15.4 to -12.9 ‰). Some of them have pronounced excursions on the isotopic composition, which indicates a difference in their genesis: from condensate to mixed with ancient infiltrogenic. The isotopic composition of carbon of water-dissolved carbon dioxide (δС from -41.6 to -16.3 ‰) indicates its biogenic origin and the possibility of interstratal flows from overlying horizons.
INDEX SPECIES OF UPPER ORDOVICIAN CONODONTS FROM THE TELETSKOE LAKESIDE ALTAI
Authors: Obut Olga T. , Sennikov Nikolay V. , Pecherichenko Dmitry A.
Published: October 2021

New data on the conodont index species from Upper Ordovician of the Teletskoe Lakeside, Gorny Altai, are presented. For the first time for this region representatives of the genera Plectodina and Belodina were found.
ISOTOPE COMPOSITION OF NATURAL WATERS IN THE VERKH-TULA SETTLEMENT (THE NOVOSIBIRSK REGION)
Authors: Pyrayev Aleksandr N. , Novikov Dmitry A. , Dultsev Fedor F. , Chernykh Anatoliy V. , Maksimova Anastasia A. , Derkachev Anton S.
Published: October 2021

The first data obtained in the isotope hydrogeochemical studies of natural waters in the Verkh-Tula settlement are presented in the work. The composition of these waters is mainly HCO Na-Mg-Ca with TDS varying from 542 to 731 mg/dm, and silicon content 0.46 to 7.04 mg/dm. The geochemical parameters of the medium vary from reductive to oxidative with Eh -157.4 - +231, weakly alkaline pH (7,4 - 8,1) and О 0.29 - 5.52 mg/dm3. It was established that δD and δ18O of surface and ground waters differ from each other substantially and vary from -105 to -126 ‰ and from -13.2 to -16.3 ‰ - for the former, and from -136 to -138 ‰, from -18.3 to -18.8 ‰ - for the latter. According to the data obtained, for the majority of groundwaters, the time of water residence in the aquifer is not less than 5 years, and their feeding is independent of local surface waters. The isotope composition of water-dissolved carbon (δС from -14.3 to -12.5 ‰) points to the biogenic origin of carbon dioxide participating in carbonate-silicate weathering.
A NEW TYPE OF INDUCTIVE TRANSIENT CHARACTERISTICS COMPLICATED BY THE INFLUENCE OF FAST-PROCESSING INDUCED POLARIZATION
Authors: Antonov Evgeniy Yu. , Kozhevnikov Nikolay O. , Korsakov Mikhail A.
Published: October 2021

The effects of induction-induced polarization with several relaxation times are discussed.
OIL AND GAS MODELING GENERARION IN THE JURASSIC SEDIMENTS IN THE SOUTH-EASTERN OF THE WEST SIBERIAN PETROLEUM PROVINSE
Authors: Glukhova Elena A. , Safronov Pavel I. , Burshtein Lev M.
Published: October 2021

The article presents the one-dimensional basin modeling performed in four wells to reconstruct the thermal history of deposits and reconstruct the effective values of the heat flow density.
FORECAST OF OIL AND GAS ACCUMULATIONS ZONES IN THE RIFEAN OIL AND GAS COMPLEX IN THE WEST OF THE CENTRAL-TUNGUSSKA OIL AND GAS BEARING REGION
Authors: Konstantinova Larisa N. , Abdulin Mikhail I.
Published: October 2021

Based on the data of deep drilling and regional seismic surveys, the correlation of Riphean sections was performed and a map of the distribution of Riphean formations on the pre-Vendian surface was constructed. Three perspective zones for the search for oil and gas deposits have been identified.
THE PROBLEM OF PHYSICAL ACTIVITY OF STUDENTS DURING THE PANDEMIC AND DISTANCE LEARNING
Authors: Teplukhin Evgeny I. , Loginova Anastasia S.
Published: October 2021

This article examines the problem of the pandemic and its participation in the physical education of students, shows and proves the progress of physical education of students who are engaged in sports on a regular basis and identifies the positive aspects of sports.
ON THE MAIN REASONS FOR THE ACTIVATION OF RIGHT-WING SOCIAL REVOLUTIONARIES IN SIBERIA AT THE BEGINNING OF THE NEP
Authors: Dobrovolsky Anatoly V.
Published: October 2021

The article reveals the reasons for the activation of the right-wing socialist-revolutionaries in Siberia in the early 1920s, after the introduction of a new economic policy in Soviet Russia. The author shows that a number of objective and subjective factors contributed to the activation of the right-wing social revolutionaries in Siberia. Among the objective factors, he points out the discontent of the population with the policy of the Soviet government during the years of "War Communism "and at the beginning of the NEP, the personnel" hunger " of the ruling Bolshevik Party, the close location of the Far Eastern Republic. The sharp intensification of the activities of socialist revolutionaries in the article is demonstrated on the example of Siberian societies, where revolutionaries "captured" the leading posts in the middle and lower managerial level, it has used the material basis of cooperation for the establishment of inner work, expand their outreach. And only the decisive actions of the leadership of the RCP( b), punitive measures of the Cheka/GPU bodies stopped the activity of the Social Revolutionaries, led to the defeat of the socialist opposition in the early 1920s.
EVERYDAY LIFE HISTORY AS A SCIENTIFIC AREA
Authors: Sotnikova Elena V.
Published: October 2021

The article presents the analysis of the discussions of researchers about the essence and major components of the category “everyday life”; the interdisciplinary character of the problem is emphasized. In particular, the spatial, object, action, value and emotional components of everyday life are characterized. The significance of the history of everyday life as a modern scientific direction is assessed.
SHUSHENSKOYE IN THE MEMOIRS OF POLITICAL EXILES
Authors: Akopyants Anait S.
Published: October 2021

The article attempts to present the image of the Siberian village of Shushenskoye at the end of the XIX century, which was one of the places of political exile in the Minusinsk district of the Yenisei province, on the basis of the available documents. Decembrists, participants of the Polish uprisings, public figures, and narodniks were exiled here at various times, but they did not leave any information about Shushenskoye. The unique interesting and expressive characteristics of the appearance, life, surroundings of Shushenskoye, flora and fauna are presented in the memoirs and correspondence of the exiled social Democrats, which are collected and presented in this publication. They, to a certain extent, break the stereotypes about Shushenskoye and about the peculiarities of political exile at the end of the XIX century, which have developed in the Soviet historical literature, and indicate that political exiles served their exile here under a fairly liberal regime and a relatively comfortable life.
WAYS OF COMMUNICATION OF WESTERN SIBERIA ON MAPS XVIII - BEGINNING XX CENTURIES
Authors: Kationov Oleg N. , Romanenko Nikita A.
Published: October 2021

Resume: This article provides a brief overview of the maps of the routes of communication in Western Siberia of the pre-revolutionary period, stored both in federal archives and libraries of the country, and in local history museums in various cities and regions of the country. The paper proposes a typology of maps of communication routes of imperial Russia. The authors offer a selection of maps showing land roads, railways, waterways, postal routes, a brief description of each presented map is given.
GEOCOGNITIVE ASPECTS OF SPATIAL DEVELOPMENT OF THE SIBERIAN REGION. STATEMENT OF A QUESTION
Authors: Osipov Alexey G. , Savinykh Vyacheslav N. , Makarenko Natalya N.
Published: October 2021

The paper considers the application of such a driver of the VI technological order as geocognitive modeling for the implementation of the spatial strategy of the socio-economic development of the Siberian region. The role of this driver for the development of new approaches in assessing the potential of the Siberian Federal District (SFD) is shown. The importance of theoretical approaches and digital tools based on artificial intelligence for the introduction of geocognitive technologies into the practice of state and corporate governance is noted. The prospect of using geocognitive tools in researching the problems of spatial economics is assessed.
DYNAMICS OF CHANGES IN THE PSYCHOPHYSIOLOGICAL STATE OF TECHNICAL UNIVERSITY STUDENTS
Authors: Klimova Elena V. , Mukhametova Olga V. , Mukhametov Nail Sh.
Published: October 2021

This paper examines the dynamics of changes in the indicators of the psychophysiological state of students of the Siberian State University of Railway Transport. The indicators of motor activity, vital index (ZHI) and respiratory system were considered. The purpose of the study is to identify the physical and functional capabilities of the body of first-year students, as well as to analyze the level of health. The objectives of the study are to determine the level of psychophysiological state of students in dynamics. Research hypothesis: the source of physical education should be on motor activity that is appropriate for development, in order to promote self-efficacy and pleasure, as well as encourage continuous participation in physical activity; through the acquisition of motivational experience by students in physical education and sports, physical and functional development is achieved, as well as self-esteem, goal orientation and tasks; the effectiveness of physical development can be achieved by changing the approaches to the implementation of FC programs, sports facilities, recruitment, as well as the organization of school-university continuity. Research methods: analysis, systematization, generalization. The results of the research showed a decrease in physical fitness and activity in general, as well as the need to develop motor abilities and improve the level of physical health of students.
PENGARUH PENERAPAN INFORMASI AKUNTANSI MANAJEMEN TERHADAP KINERJA MANAJERIAL HOTEL DI KOTA KENDARI
Authors: Safarudin Safarudin , Awaluddin Ishak , Riswan Riswan
Published: October 2021

Penelitian ini bertujuan untuk mengetahui dan menganalisis pengaruh penerapan informasi akuntansi terhadap kinerja manajerial hotel di kota kendari. Data yang digunakan adalah data primer dan sekunder. Jenis Data yang digunakan adalah data kualitatif dan data kuantitatif. Sampel penelitian yaitu 52 manajer hotel di Kota Kendari. Alat analisis yang digunakan dalam penelitian ini adalah regresi linier sederhana. Hasil penelitian ini menunjukkan bahwa penerapan informasi akuntansi manajemen berpengaruh signifikan terhadap kinerja manajerial. Artinya semakin baik penerapan informasi akuntansi manajemen maka semakin baik pula kinerja manajerial yang ada di hotel kota kendari.
Domestic Institutional Design and the National Interest in Trade Negotiations
Authors: Rodrigues Vieira Vinicius
Published: October 2021

How do different domestic institutional settings affect the formation of the national interest in trade negotiations? While current evidence suggests that institutions influence such a process even when societal groups dominate policymaking and international factors limit state choices, it remains unclear to what extent the domestic institutional design shape bureaucrats’ perceptions on both internal and foreign constraints. Building upon Brazil’s and India’s cases in World Trade Organization’s (WTO) Doha Round between 2003 and 2008, I address that question through process-tracing with the purpose of theory-building. I focus the analysis on the nodal bureaucracy, understood as the institution at the forefront of international bargaining. I argue that if such a bureaucracy is responsible for diplomacy in general, foreign factors prevail in decision-making. In turn, if the nodal bureaucracy is devoted only to trade-related issues, domestic economic factors dominate policymaking. I reach those conclusions through a paired-comparison between Brazil and India.
Hannah Arendt and Representative Democracy: Authority as a Non-Foundational Category
Authors: Luik Lelde
Published: October 2021

Hannah Arendt is a widely influential thinker, but her work has been much less associated with theories of representative democracy and political representation. This paper discusses Arendt's notion of authority as a source for 'worldly permanence and reliability' that makes a valuable contribution to non-foundationalist and constructivist perspectives on representative democracy. Situated in Arendt's framework of action, freedom and power, authority is a faculty that denotes both Arendt's temporal conception of power and the world in which it unfolds.
Voluntary Sacrifice of Freedom: Why Are Some Chinese Nationals Supporting Government Censorship?
Authors: Wu Ziyi
Published: October 2021

While the effects of government censorship are widely explored in the literature, most works on government censorship have assumed a nature intention for citizens to seek freedom of speech in authoritarian regimes. However, citizens in authoritarian regimes may actually support government censorship due to various reasons. In this study, I explore the potential reasons of citizen's support towards government censorship in China among Chinese nationals both domestic and abroad. I proposes that willingness to support censorship is influenced by one's experience with political institutions and the political environment. The government could try to provide reasonings on censorship that aligns its interest in repressive behavior and citizens' personal interests to gain support on censorship.
Authoritarian Leadership Politics and Constraints on the Executive: A Mediation Analysis of Ideology, Populism, and Civil Group Balance of Power
Authors: Hanna Tom
Published: October 2021

Why do some regimes slide further into autocracy while other regimes, similar in their formal institutions and economies, move toward democratization? What can explain the erosion of longstanding traditional institutions in autocracy? I contend that the answer lies in the ideology of the dictator with rhetorical style in a secondary role. Specifically, ideological programs of aggressive social change necessarily weaken existing civil society constraints and the reliance of the regime on ideology for legitimacy increases the power of ideological support groups. The combination of ideological agendas hostile to existing social structures with the instrumental use of populist rhetoric to bypass existing constraints is corrosive to both civil society and the institutional constraints it supports. This project will demonstrate using mediation analysis that radical leadership ideology results in the erosion of institutional constraints with changes in civil society as a causal mechanism, and that populism's role is secondary.
Identification of Hub mRNAs and lncRNAs in Atrial Fibrillation Using Weighted Co-expression Network Analysis With RNA-Seq Data
Authors: Yang Pan , Cao Yujing , Jian Huagang , Chen Hao
Published: October 2021

Atrial fibrillation (AF)/paroxysmal AF (PAF) is the main cause of cardiogenic embolism. In recent years, the progression from paroxysmal AF to persistent AF has attracted more and more attention. However, the molecular mechanism of the progression of AF is unclear. In this study, we performed RNA sequencing for normal samples, paroxysmal AF and persistent AF samples to identify differentially expressed gene (DEG) and explore the roles of these DEGs in AF. Totally, 272 differently expressed mRNAs (DEmRNAs) and 286 differentially expressed lncRNAs (DElncRNAs) were identified in paroxysmal AF compared to normal samples; 324 DEmRNAs and 258 DElncRNAs were found in persistent atrial fibrillation compared with normal samples; and 520 DEmRNAs and 414 DElncRNAs were identified in persistent AF compared to paroxysmal AF samples. Interestingly, among the DEGs, approximately 50% were coding genes and around 50% were non-coding RNAs, suggesting that lncRNAs may also have a crucial role in the progression of AF. Bioinformatics analysis demonstrated that these DEGs were significantly related to regulating multiple AF associated pathways, such as the regulation of vascular endothelial growth factor production and binding to the CXCR chemokine receptor. Furthermore, weighted gene co-expression network analysis (WGCNA) was conducted to identify key modules and hub RNAs and lncRNAs to determine their potential associations with AF. Five hub modules were identified in the progression of AF, including blue, brown, gray, turquoise and yellow modules. Interestingly, blue module and turquoise module were significantly negatively and positively correlated to the progression of AF respectively, indicating that they may have a more important role in the AF. Moreover, the hub protein-protein interaction (PPI) networks and lncRNA–mRNA regulatory network were constructed. Bioinformatics analysis on the hub PPI network in turquoise was involved in regulating immune response related signaling, such as leukocyte chemotaxis, macrophage activation, and positive regulation of α-β T cell activation. Our findings could clarify the underlying molecular changes associated fibrillation, and provide a useful resource for identifying AF marker.
Single-Cell RNA Sequencing Revealed CD14+ Monocytes Increased in Patients With Takayasu’s Arteritis Requiring Surgical Management
Authors: Qing Gao , Zhiyuan Wu , Jinge Yu , Yuqing Miao , Zuoguan Chen , Yongpeng Diao , Jinfeng Yin , Junnan Jia , Yijia Guo , Weimin Li , Yongjun Li
Published: October 2021

Objectives: Takayasu Arteritis (TA) is a highly specific vascular inflammation and poses threat to patients’ health. Although some patients have accepted medical treatment, their culprit lesions require surgical management (TARSM). This study aimed at dissecting the transcriptomes of peripheral blood mononuclear cells (PBMCs) in these patients and to explore potential clinical markers for TA development and progression.

Methods: Peripheral blood were collected from four TA patients requiring surgical management and four age-sex matched healthy donors. Single cell RNA sequencing (scRNA-seq) was adopted to explore the transcriptomic diversity and function of their PBMCs. ELISA, qPCR, and FACS were conducted to validate the results of the analysis.

Results: A total of 29918 qualified cells were included for downstream analysis. Nine major cell types were confirmed, including CD14+ monocytes, CD8+ T cells, NK cells, CD4+ T cells, B cells, CD16+ monocytes, megakaryocytes, dendritic cells and plasmacytoid dendritic cells. CD14+ monocytes (50.0 vs. 39.3%, p < 0.05) increased in TA patients, as validated by FACS results. TXNIP, AREG, THBS1, and CD163 increased in TA patients. ILs like IL-6, IL-6STP1, IL-6ST, IL-15, and IL-15RA increased in TA group.

Conclusion: Transcriptome heterogeneities of PBMCs in TA patients requiring surgical management were revealed in the present study. In the patients with TA, CD14+ monocytes and gene expressions involved in oxidative stress were increased, indicating a new treatment and research direction in this field.
Pd Doped on TCH@SBA-15 Nanocomposites: Fabrication and Application as a New Organometallic Catalyst in the Three-Component Synthesis of N-Benzo-imidazo- or -thiazole-1,3-thiazolidinones
Authors: Kalhor Mehdi , Dadras Akbar
Published: October 2021

In this study, Pd(II)/TCH@SBA-15 nanocomposites were synthesized by the grafting of 3-chloropropyltriethoxysilane and thiocarbohydrazide on SBA-15 and subsequent deposition of palladium acetates through the ligand–metal coordination method. The structure and morphology of this nanoporous nanocomposite was thoroughly identified by Fourier transform infrared spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, thermogravimetric analysis, atomic absorption spectroscopy, and Brunauer–Emmett–Teller instrumental analyses. Furthermore, the catalytic activity of this nanocomposite was investigated in the three-component synthesis of 3-benzimidazolyl or benzothiazoleyl-1,3-thiazolidin-4-ones via a reaction of 2-aminobenzimidazole or 2-aminobenzothiazole, aromatic aldehydes, and thioglycolic acid in an acetone–H2O mixture under green conditions. The Pd/TCH@SBA-15 nanocatalyst is demonstrated to exhibit a high catalyzing activity in the three-component reaction of the synthesis of N-heterocyclic thiazolidinones with good to excellent yields. One of the advantages of the suggested method is the direct application of the thiocarbohydrazide ligand to stabilize Pd nanoparticles through formation of a stable ring complex without creating an additional Schiff base step. Moreover, this organometallic nanocatalyst can be recycled several times with no notable leaching or loss of performance.
Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis
Authors: Wang Tian-Qi , Li Ling-Ru , Tan Chun-Xia , Yang Jing-Wen , Shi Guang-Xia , Wang Li-Qiong , Hu Hui , Liu Zhi-Shun , Wang Jun , Wang Tong , Yuan Yong , Jia Wen-Rui , Li Hua , Wang Xin-Wei , Wu Bin , Tu Jian-Feng , Liu Cun-Zhi
Published: October 2021

A close relationship between knee osteoarthritis (KOA) and gut microbiota has recently been described. Herein, we aim to investigate the effect of electroacupuncture (EA) on gut microbiota in participants with KOA. We conducted a study of 60 participants with KOA and 30 matched healthy controls (HCs). Sixty participants were allocated to either EA group (n=30) or sham acupuncture (SA) group (n=30). Five obligatory acupoints and three adjunct acupoints were punctured in the EA group. Eight non-acupoints that were separated from conventional acupoints or meridians were used for the SA group. Participants in both groups received 24 sessions within eight weeks. Fecal microbial analyses by 16S ribosomal RNA gene sequencing were carried out after collecting stools at T0 and T8 weeks (Four samples with changed defecation habits were excluded). The results showed that both Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score (P=0.043) and NRS score (P=0.002) decreased more in EA group than those in SA group. Moreover, EA could reverse more KOA-related bacteria including Bacteroides, [Eubacterium]_hallii_group, Agathobacter and Streptococcus. The number of significantly different genera between KOA patients and HCs were less after EA treatment than that after SA treatment. This meant that EA modified the composition of the gut microbiome, making it closer to healthy people, while not significantly affecting the microbial diversity. Two genera including Agathobacter (P=0.0163), Lachnoclostridium (P=0.0144) were statistically increased than baseline in EA group (paired Wilcoxon rank sum test). After EA treatment, Bacteroides (P=0.0394) was more abundant and Streptococcus (P=0.0306) was significantly reduced in patients who demonstrated adequate response than in those with inadequate response (Wilcoxon rank-sum test). Spearman correlation test between gut microbe and KOA clinical outcomes indicated that Bacteroides and Agathobacter was negatively correlated with NRS score, WOMAC total score, and WOMAC pain, stiffness and pain scores (P<0.001 or 0.05 or 0.01), while Streptococcus was positively correlated with them (P<0.05 or 0.01). Our study suggests that EA contributes to the improvement of KOA and gut microbiota could be a potential therapeutic target.
Interaction With the Extracellular Matrix Triggers Calcium Signaling in Trypanosoma cruzi Prior to Cell Invasion
Authors: Manchola Varón Nubia Carolina , dos Santos Guilherme Rodrigo R. M. , Colli Walter , Alves Maria Julia M.
Published: October 2021

Trypanosoma cruzi, the etiological agent of Chagas disease in humans, infects a wide variety of vertebrates. Trypomastigotes, the parasite infective forms, invade mammalian cells by a still poorly understood mechanism. Adhesion of tissue culture- derived trypomastigotes to the extracellular matrix (ECM) prior to cell invasion has been shown to be a relevant part of the process. Changes in phosphorylation, S-nitrosylation, and nitration levels of proteins, in the late phase of the interaction (2 h), leading to the reprogramming of both trypomastigotes metabolism and the DNA binding profile of modified histones, were described by our group. Here, the involvement of calcium signaling at a very early phase of parasite interaction with ECM is described. Increments in the intracellular calcium concentrations during trypomastigotes-ECM interaction depends on the Ca2+ uptake from the extracellular medium, since it is inhibited by EGTA or Nifedipine, an inhibitor of the L-type voltage gated Ca2+ channels and sphingosine-dependent plasma membrane Ca2+ channel, but not by Vanadate, an inhibitor of the plasma membrane Ca2+-ATPase. Furthermore, Nifedipine inhibits the invasion of host cells by tissue culture- derived trypomastigotes in a dose-dependent manner, reaching 95% inhibition at 100 µM Nifedipine. These data indicate the importance of both Ca2+ uptake from the medium and parasite-ECM interaction for host-cell invasion. Previous treatment of ECM with protease abolishes the Ca2+ uptake, further reinforcing the possibility that these events may be connected. The mitochondrion plays a relevant role in Ca2+ homeostasis in trypomastigotes during their interaction with ECM, as shown by the increment of the intracellular Ca2+ concentration in the presence of Antimycin A, in contrast to other calcium homeostasis disruptors, such as Cyclopiazonic acid for endoplasmic reticulum and Bafilomycin A for acidocalcisome. Total phosphatase activity in the parasite decreases in the presence of Nifedipine, EGTA, and Okadaic acid, implying a role of calcium in the phosphorylation level of proteins that are interacting with the ECM in tissue culture- derived trypomastigotes. In summary, we describe here the increment of Ca2+ at an early phase of the trypomastigotes interaction with ECM, implicating both nifedipine-sensitive Ca2+ channels in the influx of Ca2+ and the mitochondrion as the relevant organelle in Ca2+ homeostasis. The data unravel a complex sequence of events prior to host cell invasion itself.
Unintended Consequences: Unknowable and Unavoidable, or Knowable and Unforgivable?
Authors: Suckling James , Hoolohan Claire , Soutar Iain , Druckman Angela
Published: October 2021

Recognizing that there are multiple environmental limits within which humanity can safely operate, it is essential that potential negative outcomes of seemingly positive actions are accounted for. This alertness to unintended consequences underscores the importance of so called “nexus” research, which recognizes the integrated and interactive nature of water, energy and food systems, and aims to understand the broader implications of developments in any one of these systems. This article presents a novel framework for categorizing such detrimental unintended consequences, based upon how much is known about the system in question and the scope for avoiding any such unintended consequences. The framework comprises four categories (Knowable and Avoidable; Knowable and Unavoidable; Unknowable and Avoidable, and Unknowable and Unavoidable). The categories are explored with reference to examples in both the water-energy-food nexus and planetary boundary frameworks. The examples highlight the potential for the unexpected to happen and explore dynamic nature of the situations that give rise to the unexpected. The article concludes with guidance on how the framework can be used to increase confidence that best efforts have been made to navigate our way toward secure and sustainable water, energy and food systems, avoiding and/or managing unintended consequences along the way.
Sex Differences in Platelet Reactivity in Patients With ST-Elevation Myocardial Infarction: A Sub-Analysis of the ON-TIME 3 Trial
Authors: Tavenier Anne H. , Hermanides Renicus S. , Ottervanger Jan Paul , Belitser Svetlana V. , Klungel Olaf H. , Appelman Yolande , van Leeuwen Maarten A.H. , van 't Hof Arnoud W.J.
Published: October 2021

Background: Fast and adequate platelet inhibition is one of the cornerstones in the treatment of patients with ST-elevation myocardial infarction (STEMI). The aim of this analysis is to examine sex differences in platelet inhibition in the acute treatment of STEMI patients.

Methods: Platelet reactivity units (PRU) and ticagrelor plasma concentrations of all patients in the ON-TIME 3 were compared according to sex. All patients were pre-treated with crushed ticagrelor, aspirin and heparin. Both univariable and multivariable analyses were performed.

Results: In this sub-analysis of the ON-TIME 3 trial, 195 STEMI patients, of which 58 female patients (29.7%) and 137 male patients (70.3%), were analyzed. PRU-values immediately post-PCI were not different in females [median 135 (IQR 47-228)] compared to males [160 (IQR 40-219), P = 0.92]. Ticagrelor plasma concentrations were higher in the females at the start of primary PCI [141 ng/mL (IQR 25-491) vs. 76 ng/mL (IQR 15-245), P = 0.049] and at 6 hours post-primary PCI [495 ng/mL (IQR 283-661) vs. 321 ng/mL (IQR 196-537), P = 0.001] compared to males. However, immediately post-primary PCI and at 1-hour post-primary PCI no significant differences in ticagrelor concentrations were seen between sexes. In multivariable analysis, sex was significantly associated with ticagrelor concentration (P = 0.04), but not with PRU (P = 0.93).

Conclusion: Effective platelet inhibition reached by crushed ticagrelor in STEMI patients was similar in both sexes. Females had similar or even higher ticagrelor plasma concentrations up to 6 hours post-primary PCI compared with males.
Pulmonary Artery Stump Thrombosis: To Treat or Not to Treat? The Question Is Still Open. Description of a Case and Review of the Literature
Authors: Mirijello Antonio , Santoliquido Mariateresa , Piscitelli Pamela , Borelli Cristina , Serviddio Gaetano , Simeone Anna , Grandone Elvira , De Cosmo Salvatore
Published: October 2021

Pulmonary artery stump thrombosis (PAST) represents a possible complication after lung surgery. We report the case of a 59-year-old man who presented with dyspnoea about 4 years after right pneumonectomy due to squamous cell lung cancer. A CT-scan showed the presence of pulmonary artery stump thrombosis. Although there was no evidence of pulmonary embolism, given the clinical features and radiological shape of the thrombus, anticoagulation treatment with low-molecular-weight heparin was started with improvement of symptoms. The patient was discharged on anticoagulant treatment and a pulmonary CT-scan performed 4 months later showed an almost complete resolution of the PAST. Pathophysiological mechanisms of PAST are still unknown, although several hypotheses have been proposed. However, the decision to treat PAST with anticoagulants is still controversial. A review of literature will be provided in order to discuss risk factors, possible etiologies and to highlight clinical and radiological characteristics that could suggest to treat this condition, in particular when there is an increased risk of complications.
Similar Inflammatory Biomarkers Reflect Different Platelet Reactivity in Percutaneous Coronary Intervention Patients Treated With Clopidogrel: A Large-Sample Study From China
Authors: Li Jiawen , Yuan Deshan , Jiang Lin , Tang Xiaofang , Xu Jingjing , Song Ying , Chen Jue , Qiao Shubin , Yang Yuejin , Gao Runlin , Xu Bo , Yuan Jinqing , Zhao Xueyan
Published: October 2021

Background: Platelet reactivity is closely associated with adverse events in percutaneous coronary intervention (PCI) patients. Inflammation plays a crucial role in the development of coronary heart disease (CHD).

Aim: To investigate the association of inflammatory biomarkers such as leukocyte count and high-sensitivity C reactive proteins (hs-CRP) with platelet reactivity in PCI patients treated with clopidogrel.

Method: We examined 10,724 consecutive PCI patients in Fuwai hospital from January 2013 to December 2013. High on-treatment platelet reactivity (HTPR) was defined as adenosine diphosphate (ADP)-induced platelet maximum amplitude [MA(ADP)] of thromboelastogram (TEG) > 47 mm, and low on-treatment platelet reactivity (LTPR) MA(ADP) < 31 mm.

Results: Finally, 6,772 PCI patients treated with clopidogrel who had the results of postoperative TEG were enrolled. Among them, 2,070 (30.57%) presented HTPR and 2,568 (37.92%) presented LTPR. As for LTPR, multivariate logistic regression showed that leukocyte count (OR: 1.153, 95% CI 1.117–1.191) and hs-CRP (OR: 0.920, 95% CI 0.905–0.936) were independent predictors, along with diabetes mellites, hemoglobin, platelet count and glucose. As for HTPR, multivariate logistic regression showed that leukocyte count (OR: 0.885, 95% CI 0.854–0.917) and hs-CRP (OR: 1.094, 95% CI 1.077–1.112) were independent predictors, along with sex, hemoglobin, platelet count and glucose.

Conclusions: This was the first large real-world study reporting that both leukocyte count and hs-CRP were the independent factors for platelet reactivity in PCI populations treated with clopidogrel, among which higher leukocyte count was associated with more LTPR while higher hs-CRP was associated with more HTPR, providing new insights on individualized antiplatelet therapy.
Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children
Authors: Villhauer Alissa , Lynch David , Postler Taylor , Dawson Deborah , Drake David
Published: October 2021

Aim: The purpose of this study was to investigate the presence of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus species in an American Indian population displaying a high incidence of severe early childhood caries (S-ECC) and to explore the genotypic diversity and fidelity of transmission of S. sobrinus in this population.

Methods: We report here on the microbial profiles of 71 children compiled from birth to 36 months of age and initial exploration of genotypic diversity in a subset of 40 mother/child dyads. Whole mouth plaque samples were collected from mother/child dyads in a Northern Plains American Indian cohort. Mutans streptococci (MS) and Lactobacillus counts were recorded and presence/absence of S. mutans, S. sobrinus, and Lactobacillus was noted for each child. S. sobrinus was isolated and genotyped via arbitrarily primed-polymerase chain reaction (AP-PCR).

Results: The children in this study were divided into caries active (CA) and caries free (CF) groups. S. mutans, S. sobrinus, and Lactobacillus species were detected more frequently in CA groups. Distribution of MS and Lactobacillus counts was significantly different between the two groups, as was the distribution of three species colonization profiles. Twelve S. sobrinus genotypes were detected in the subset of mother/child dyads. Individual participants displayed a range of 0–3 distinct genotypes while mother/child pairs displayed a range of 1–5 genotypes. Eighty-three percent of children shared at least one genotype with his/her mother.

Conclusion: Caries active children in this population display different microbial profiles than the caries free children in this cohort. A small number of S. sobrinus genotypes appear to be very common in this population. A high rate of vertical transmission of S. sobrinus from mother to child is seen in these mother/child dyads.
Edge Intelligent Perception Method for Power Grid Icing Condition Based on Multi-Scale Feature Fusion Target Detection and Model Quantization
Authors: Ma Fuqi , Wang Bo , Li Min , Dong Xuzhu , Mao Yifan , Zhou Yinyu , Ma Hengrui
Published: October 2021

Insulator is an important equipment of power transmission line. Insulator icing can seriously affect the stable operation of power transmission line. So insulator icing condition monitoring has great significance of the safety and stability of power system. Therefore, this paper proposes a lightweight intelligent recognition method of insulator icing thickness for front-end ice monitoring device. In this method, the residual network (ResNet) and feature pyramid network (FPN) are fused to construct a multi-scale feature extraction network framework, so that the shallow features and deep features are fused to reduce the information loss and improve the target detection accuracy. Then, the full convolution neural network (FCN) is used to classify and regress the iced insulator, so as to realize the high-precision identification of icing thickness. Finally, the proposed method is compressed by model quantization to reduce the size and parameters of the model for adapting the icing monitoring terminal with limited computing resources, and the performance of the method is verified and compared with other classical method on the edge intelligent chip.
Physicochemical Factors Drive Bacterial Communities in an Aquaculture Environment
Authors: Sun Fulin , Wang Chunzhong , Yang Hongqiang
Published: October 2021

The role of microbial ecology in aquaculture is becoming increasingly significant; however, studies on the changes in microbial ecology driven by the culture environment are limited. In the present study, high-throughput sequencing and chemical analysis was used to explore changes in physicochemical factors, bacterial communities, and their relationships between a water source (Bay) and an aquaculture area located in a reclaimed area. Statistical analysis results revealed that operational taxonomic units levels in inlet water and pond water varied significantly (p < 0.05). Non-metric multidimensional scaling (NMDS) analysis revealed the distribution characteristics of bacterial communities with water properties. The abundance of Alphaproteobacteria, Actinobacteria, and Flavobacteria in pond water increased significantly when compared to inlet water. The abundance of heterotrophic bacteria, such as Candidatus Actinomarina, Candidatus Aquiluna, Marivita, and Vibrio genera in pond water was significantly higher (Welch’s t-tests, p < 0.05) than inlet water. Functional prediction analysis primarily revealed an increase in the function that was associated with carbon and nitrogen metabolism in the pond environment. Canonical correlation analysis revealed that the bacterial communities was predominantly influenced by inorganic nutrients. Nitrate-nitrogen (N), nitrite-N, ammonium-N, and phosphate-phosphorous (P) were the key factors influencing bacterial communities in pond environment. A significant correlation was observed between inorganic N and phosphorus (P), and dominant bacterial genera (p < 0.05), demonstrating the potential mechanism of regulation of nutrients in bacterial communities. The present study described the microbial ecology of aquaculture ponds in detail and provides a scientific basis for the management of aquacultural environments.