bluuebunny/crossref_metadata_2025 huggingface dataset:
An Overview of Sensory Characterization Techniques: From Classical Descriptive Analysis to the Emergence of Novel Profiling Methods
Authors: Marques Catarina , Correia Elisete , Dinis Lia-Tânia , Vilela Alice
Published: January 2022

Sensory science provides objective information about the consumer understanding of a product, the acceptance or rejection of stimuli, and the description of the emotions evoked. It is possible to answer how consumers perceive a product through discriminative and descriptive techniques. However, perception can change over time, and these fluctuations can be measured with time-intensity methods. Instrumental sensory devices and immersive techniques are gaining headway as sensory profiling techniques. The authors of this paper critically review sensory techniques from classical descriptive analysis to the emergence of novel profiling methods. Though research has been done in the creation of new sensory methods and comparison of those methods, little attention has been given to the timeline approach and its advantages and challenges. This study aimed to gather, explain, simplify, and discuss the evolution of sensory techniques.
Pasta-Making Process: A Narrative Review on the Relation between Process Variables and Pasta Quality
Authors: Bresciani Andrea , Pagani Maria Ambrogina , Marti Alessandra
Published: January 2022

Pasta is an increasingly popular food worldwide and different formulations have been developed to improve its nutritional profile. Semolina that is high both in protein and gluten content is recognized as the ideal raw material to produce conventional dry pasta. When alternative raw materials are used, an understanding of the relationship between processing variables and pasta quality is crucial in order to optimize the redesign of the production process. This review aims to: (1) investigate the main challenges of the pasta-making process, highlighting the processing variables that most affect pasta quality; and (2) indicate the unknown factors that influence the pasta-making process and which need to be studied. After overviewing the last twenty years of research in the pasta sector, the interplay/relationship between processing variables and pasta quality is examined, together with the main innovations proposed for each step of pasta processing. An analysis of all the variables involved in the process and their influence on each other will elucidate how to optimize certain parameters to ensure the production of pasta with the desired characteristics.
DNA-Based Tools to Certify Authenticity of Rice Varieties—An Overview
Authors: Vieira Maria Beatriz , Faustino Maria V. , Lourenço Tiago F. , Oliveira M. Margarida
Published: January 2022

Rice (Oryza sativa L.) is one of the most cultivated and consumed crops worldwide. It is mainly produced in Asia but, due to its large genetic pool, it has expanded to several ecosystems, latitudes and climatic conditions. Europe is a rice producing region, especially in the Mediterranean countries, that grow mostly typical japonica varieties. The European consumer interest in rice has increased over the last decades towards more exotic types, often more expensive (e.g., aromatic rice) and Europe is a net importer of this commodity. This has increased food fraud opportunities in the rice supply chain, which may deliver mixtures with lower quality rice, a problem that is now global. The development of tools to clearly identify undesirable mixtures thus became urgent. Among the various tools available, DNA-based markers are considered particularly reliable and stable for discrimination of rice varieties. This review covers aspects ranging from rice diversity and fraud issues to the DNA-based methods used to distinguish varieties and detect unwanted mixtures. Although not exhaustive, the review covers the diversity of strategies and ongoing improvements already tested, highlighting important advantages and disadvantages in terms of costs, reliability, labor-effort and potential scalability for routine fraud detection.
Effect of High-Pressure Processing on Fresh Sea Urchin Gonads in Terms of Shelf Life, Chemical Composition, and Microbiological Properties
Authors: Coroneo Valentina , Corrias Francesco , Brutti Andrea , Addis Piero , Scano Efisio , Angioni Alberto
Published: January 2022

Paracentrotus lividus is a widespread sea urchin species appreciated worldwide for the taste of its fresh gonads. High-pressure processing (HPP) can provide a thermal equivalent to pasteurization, maintaining the organoleptic properties of the raw gonads. This study evaluated HPP technology’s effect at 350 MPa and 500 MPa on microbial inactivation and biochemical characteristics of P. lividus gonads. HPP at 350 MPa resulted in a higher decrease in protein and free amino acids associated with a loss of olfactory, color, and gustatory traits and a visual alteration of the texture. On the other hand, gonad samples stored for 40 days after treatments at 500 MPa showed a good organoleptic profile similar to fresh gonads. Furthermore, only 500 MPa effectively reduced mesophilic bacteria contamination among the two HPP treatments carried out. Total lipids increased during storage; however, the SAFA/PUFA rate was homogeneous during HPP trials ranging from 2.61–3.91 g/100 g. Total protein decreased more than 40% after HPP at 350 MPa, whereas, after 500 MPa, it remained stable for 20 days. The amount of free amino acid constantly decreased during storage after HPP at 350 MPa and remained constant at 500 MPa. HPP can effectively remove the bacterial flora and inactivate enzymes, maintaining the properties of the fresh sea urchin gonads.
Melatonin Treatment Improves Postharvest Preservation and Resistance of Guava Fruit (Psidium guajava L.)
Authors: Fan Silin , Xiong Tiantian , Lei Qiumei , Tan Qinqin , Cai Jiahui , Song Zunyang , Yang Meiyan , Chen Weixin , Li Xueping , Zhu Xiaoyang
Published: January 2022

Guava fruit has a short postharvest shelf life at room temperature. Melatonin is widely used for preservation of various postharvest fruit and vegetables. In this study, an optimal melatonin treatment (600 μmol·L−1, 2 h) was identified, which effectively delayed fruit softening and reduced the incidence of anthracnose on guava fruit. Melatonin effectively enhanced the antioxidant capacity and reduced the oxidative damage to the fruit by reducing the contents of superoxide anions, hydrogen peroxide and malondialdehyde; improving the overall antioxidant capacity and enhancing the enzymatic antioxidants and non-enzymatic antioxidants. Melatonin significantly enhanced the activities of catalase, superoxide dismutase, ascorbate peroxidase and glutathione reductase. The contents of total flavonoids and ascorbic acid were maintained by melatonin. This treatment also enhanced the defense-related enzymatic activities of chitinase and phenylpropanoid pathway enzymes, including phenylalanine ammonia lyase and 4-coumaric acid-CoA-ligase. The activities of lipase, lipoxygenase and phospholipase D related to lipid metabolism were repressed by melatonin. These results showed that exogenous melatonin can maintain the quality of guava fruit and enhance its resistance to disease by improving the antioxidant and defense systems of the fruit.
Low Temperatures Affect the Physiological Status and Phytochemical Content of Flat Leaf Kale (Brassica oleracea var. acephala) Sprouts
Authors: Šamec Dunja , Ljubej Valentina , Redovniković Ivana Radojčić , Fistanić Stjepana , Salopek-Sondi Branka
Published: January 2022

Consumption of plants in the juvenile stage becomes popular because sprouts are easy to grow, and they can be a tasty source of micro- and macro-nutrients and various phytochemicals. However, some environmental factors during sprout growth can affect their characteristics. In this article, we investigated how low temperatures during cultivation (8 °C) and additional exposure to freezing temperatures (−8 °C) affect the physiological status and phytochemical content of kale (Brassica oleracea var. acephala) sprouts compared to the control grown at 21 °C. We conducted five independent laboratory experiments and found that low temperature significantly increased proline content and decreased sprouts yield. In addition, low temperature caused a significant decrease in carotenoid and flavonoid content, while phenolic acid content and total glucosinolates content increased, but individual glucosinolates were differentially affected. Our results indicate that low temperatures affect the physiological status of kale sprouts and affect the content of phytochemicals.
UV and Visible Spectrum LED Lighting as Abiotic Elicitors of Bioactive Compounds in Sprouts, Microgreens, and Baby Leaves—A Comprehensive Review including Their Mode of Action
Authors: Artés-Hernández Francisco , Castillejo Noelia , Martínez-Zamora Lorena
Published: January 2022

Background: According to social demands, the agri-food industry must elaborate convenient safe and healthy foods rich in phytochemicals while minimising processing inputs like energy consumption. Young plants in their first stages of development represent great potential. Objective: This review summarises the latest scientific findings concerning the use of UV and visible spectrum LED lighting as green, sustainable, and low-cost technologies to improve the quality of sprouts, microgreens, and baby leaves to enhance their health-promoting compounds, focusing on their mode of action while reducing costs and energy. Results: These technologies applied during growing and/or after harvesting were able to improve physiological and morphological development of sprouted seeds while increasing their bioactive compound content without compromising safety and other quality attributes. The novelty is to summarise the main findings published in a comprehensive review, including the mode of action, and remarking on the possibility of its postharvest application where the literature is still scarce. Conclusions: Illumination with UV and/or different regions of the visible spectrum during growing and shelf life are good abiotic elicitors of the production of phytochemicals in young plants, mainly through the activation of specific photoreceptors and ROS production. However, we still need to understand the mechanistic responses and their dependence on the illumination conditions.
Fifty Years of Eclipsing Binary Analysis with the Wilson–Devinney Model
Authors: Kallrath Josef
Published: January 2022

The Wilson–Devinney model has—over the last 50 years—become the standard in analyzing eclipsing binary observations. To provide orientation for both active binary and non-binary researchers, it is presented here in historical and on-going as well as astrophysical perspectives. Among the important advances that originated with the model are: the representation of star surfaces as equipotentials for circular and eccentric orbits, leading to four morphological types; simultaneous least-squares light and velocity curve analyses; efficient reflection computation, including multiple reflection; disk theory and disk modeling. Solutions in physical units allowed for the accurate estimation of parameters such as stellar masses and photometric distances; inclusion of types of observables, properly weighted.
Effects of a Reminiscence Program on Meaning of Life, Sense of Coherence and Coping in Older Women Living in Nursing Homes during COVID-19
Authors: Sales Alicia , Pinazo-Hernandis Sacramento , Martinez Dolores
Published: January 2022

Aging is a dynamic process that can bring well-being but also physical and cognitive decline. Older adults can draw on their personal resources to help them cope and thrive through the aging process. Having personal resources to cope and ensure older adults’ well-being is important. Psychological strengths such as a sense of coherence, resilience, and coping are protective against the adversity associated with health problems such as those stemming from the COVID-19 pandemic. Our study’s purpose was to investigate the usefulness of reminiscence therapy for older women living in nursing homes during the COVID-19 pandemic. A sample composed of 29 older women was evaluated with the Purpose-in-Life Test (PIL), Sense of Coherence (SOC-13) and Brief Cope Inventory (COPE-28). Our reminiscence program consisted of 10 sessions lasting 60 min each. Reminiscence therapy is a psychological intervention for older adults to assist in remembering and interpreting the life events, feelings, and thoughts that define and give meaning to the person’s life. Reminiscence can lead to positive mental health and other elements of particular relevance to older adults. In each session, we worked on a different theme that promoted the memory of positive emotions: optimal experience, decisive moment, stresses, tensions, problems and solutions, memories of childhood, adolescence, maturity, significant people in life, sense of life, and future script. We compared an intervention group (n = 12) with a control group (n = 17) using a pre-post, single-blind design. Significant results were obtained and showed that reminiscence therapy was effective in increasing meaning of life, sense of coherence, and coping in older women. The reminiscence therapy applied yielded positive effects in older female participants living in a nursing home during COVID-19 pandemic.
Applying Discrete Event Simulation to Reduce Patient Wait Times and Crowding: The Case of a Specialist Outpatient Clinic with Dual Practice System
Authors: Fun Weng Hong , Tan Ee Hong , Khalid Ruzelan , Sararaks Sondi , Tang Kar Foong , Ab Rahim Iqbal , Md. Sharif Shakirah , Jawahir Suhana , Sibert Raoul Muhammad Yusof , Nawawi Mohd Kamal Mohd
Published: January 2022

Long wait times and crowding are major issues affecting outpatient service delivery, but it is unclear how these affect patients in dual practice settings. This study aims to evaluate the effects of changing consultation start time and patient arrival on wait times and crowding in an outpatient clinic with a dual practice system. A discrete event simulation (DES) model was developed based on real-world data from an Obstetrics and Gynaecology (O&G) clinic in a public hospital. Data on patient flow, resource availability, and time taken for registration and clinic processes for public and private patients were sourced from stakeholder discussion and time-motion study (TMS), while arrival times were sourced from the hospital’s information system database. Probability distributions were used to fit these input data in the model. Scenario analyses involved configurations on consultation start time/staggered patient arrival. The median registration and clinic turnaround times (TT) were significantly different between public and private patients (p < 0.01). Public patients have longer wait times than private patients in this study’s dual practice setting. Scenario analyses showed that early consultation start time that matches patient arrival time and staggered arrival could reduce the overall TT for public and private patients by 40% and 21%, respectively. Similarly, the number of patients waiting at the clinic per hour could be reduced by 10–21% during clinic peak hours. Matching consultation start time with staggered patient arrival can potentially reduce wait times and crowding, especially for public patients, without incurring additional resource needs and help narrow the wait time gap between public and private patients. Healthcare managers and policymakers can consider simulation approaches for the monitoring and improvement of healthcare operational efficiency to meet rising healthcare demand and costs.
Perspectives on Mass Media and Governmental Measures during the 2020 COVID-19 Pandemic in a Romanian Sample of Healthcare Practitioners
Authors: Reisz Daniela , Crișan Iulia
Published: January 2022

Objective: Along with the rapid spread of the COVID-19 pandemic around the globe, a proliferation of mass media information exposed the population to an infodemic with various implications documented worldwide. The present study analyzed Romanian healthcare practitioners’ (HCPs) appraisal of COVID-19 mass media information and governmental measures throughout 2020, ranking vaccination priorities and moral values. Methods: 97 HCP completed a cross-sectional survey with items referring to the COVID-19 pandemic in 2020. Results: Findings were consistent with other studies, indicating an overall negative appraisal of mass media information, which predicted anxiety and relaxation difficulties. Unlike other studies, our sample reported a moderate level of satisfaction with official measures in 2020, which was not related to their view on mass media information. The ranking of population categories in the vaccination order showed similarities with the governmental vaccination program in 2021. Despite placing freedom third after health and love in the hierarchy of values, HCPs showed a high tendency of limiting individual liberty for the common good. Conclusions: Results showed a dissociation between the overall negative appraisal of mass media information and the satisfaction with governmental measures in 2020. Romanian HCPs shared a secular perspective on moral values and assumed an authoritarian position.
Effect of Sustainable Preharvest and Postharvest Techniques on Quality and Storability of High-Acidity ‘Reinette du Canada’ Apple
Authors: Guerra Marcos , Sanz Miguel Ángel , Rodríguez-González Álvaro , Casquero Pedro Antonio
Published: January 2022

Consumers are more disposed towards paying price premiums for sustainable food products. This fact is especially important for products with a PDO (Protected Designation of Origin) label, such as the high-acidity apple cultivar ‘Reinette du Canada’. However, ‘Reinette du Canada’ apple fruit is affected by two of the main post-harvest disorders in apple cultivars, bitter pit and senescent breakdown. As a means to achieve sustainable production of this high-quality apple cultivar, the effect of a sustainable preharvest technique, summer pruning, in combination with a zero-residue postharvest treatment allowed in organic farming on calcium-related disorders and quality in global terms in this PDO-labelled apple cultivar was assessed during storage. The combination of the two sustainable practices was an effective tool in diminishing senescent breakdown from 43% to 17% and to decrease bitter pit incidence by up to 21% (from 36% to 15%) throughout storage, especially in fruit with high susceptibility to this disorder during storage. Moreover, the overall acceptability was kept high (values of more than 3 on a 5-point scale) when applying this environment-friendly sustainable horticulture practice, which will not only reduce the synthetic pesticide input in the apple agroecosystem, but also contribute to producing fruit with zero residues.
Delay of 1-MCP Treatment on Post-Harvest Quality of ‘Bosc Kobak’ Pear
Authors: Nguyen Lien Le Phuong , Pham Tung Thanh , Syium Zinabu Hailu , Zsom-Muha Viktória , Baranyai László , Zsom Tamás , Hitka Géza
Published: January 2022

Information about 1-MCP application time on pears is crucial to optimize the schedule of treatment in commercial practice. In the present work, the effect of a 3, 5 and 7 d delay of 1-MCP treatment after harvest on ‘Bosc Kobak’ pears was investigated, with an emphasis on shelf life. Fruit was treated with 1-MCP (625 ppm) on the 3rd, 5th and 7th d after harvest for 24 h and then kept at 20 °C for 14 d or stored at 1 °C in normal atmosphere for 6 months. Ethylene, carbon dioxide production, flesh firmness, soluble solid content and color index was determined after 4 and 6 months of cold storage and an additional 7 d of shelf-life at 20 °C. Pears treated with 1-MCP had lower values in ethylene and carbon dioxide production after storage compared to the control group; in particular, fruit treated on the 3rd d after harvest obtained the lowest values. Moreover, flesh firmness of treated samples had a higher value than that of the others after cold storage and subsequent 7 d of shelf life at 20 °C. In addition, fruit treated on the 3rd d after harvest exhibited the slower change in surface color than that of other groups. However, 1-MCP treatment on the 5th and 7th d after harvest had a minor effect on firmness and surface color change after a long storage period. The effect of 1-MCP treatment depends on the time from harvest to application. The application of 1-MCP on the 3rd d after harvest obtained the highest efficacy. The results of this study provided information on scheduling the commercial 1-MCP application for ‘Bosc Kobak’ pears.
An Indoor Space Subspacing Framework for Implementing a 3D Hierarchical Network-Based Topological Data Model
Authors: Claridades Alexis Richard C. , Choi Hyun-Sang , Lee Jiyeong
Published: January 2022

Because requirements for establishing spatial data for location-based services (LBS) have increased in demand together with an increase in the number of complex structures, especially in urban areas, research has revisited the limitations of data models in representing space. Though research and corresponding applications continue to explore indoor spaces, their complete and accurate representation remains a challenge. Indoor space presents a hierarchical structure, but, unlike their topological relationships, data models have overlooked this. As subspacing presents a method to express this hierarchy of space, we aimed to develop a subspacing framework for expressing topological and hierarchical relationships at various levels of indoor space. We accomplished this by investigating the hierarchy of indoor space structures and how this relates to implementing a multi-level Node-Relation Structure (NRS) representation of indoor space through subspacing. Furthermore, we formalized these concepts by extending the IndoorGML core model. Then, we demonstrated the potential of the proposed framework through an experiment on sample data by generating corresponding network representations at different levels of detail.
Heme A Synthase Deficiency Affects the Ability of Bacillus cereus to Adapt to a Nutrient-Limited Environment
Authors: Chateau Alice , Alpha-Bazin Béatrice , Armengaud Jean , Duport Catherine
Published: January 2022

The branched aerobic respiratory chain in Bacillus cereus comprises three terminal oxidases: cytochromes aa3, caa3, and bd. Cytochrome caa3 requires heme A for activity, which is produced from heme O by heme A synthase (CtaA). In this study, we deleted the ctaA gene in B. cereus AH187 strain, this deletion resulted in loss of cytochrome caa3 activity. Proteomics data indicated that B. cereus grown in glucose-containing medium compensates for the loss of cytochrome caa3 activity by remodeling its respiratory metabolism. This remodeling involves up-regulation of cytochrome aa3 and several proteins involved in redox stress response—to circumvent sub-optimal respiratory metabolism. CtaA deletion changed the surface-composition of B. cereus, affecting its motility, autoaggregation phenotype, and the kinetics of biofilm formation. Strikingly, proteome remodeling made the ctaA mutant more resistant to cold and exogenous oxidative stresses compared to its parent strain. Consequently, we hypothesized that ctaA inactivation could improve B. cereus fitness in a nutrient-limited environment.
2-Acetamido-2-deoxy-d-glucono-1,5-lactone Sulfonylhydrazones: Synthesis and Evaluation as Inhibitors of Human OGA and HexB Enzymes
Authors: Kiss Mariann , Timári István , Barna Teréz , Mészáros Zuzana , Slámová Kristýna , Bojarová Pavla , Křen Vladimír , Hayes Joseph M. , Somsák László
Published: January 2022

Inhibition of the human O-linked β-N-acetylglucosaminidase (hOGA, GH84) enzyme is pharmacologically relevant in several diseases such as neurodegenerative and cardiovascular disorders, type 2 diabetes, and cancer. Human lysosomal hexosaminidases (hHexA and hHexB, GH20) are mechanistically related enzymes; therefore, selective inhibition of these enzymes is crucial in terms of potential applications. In order to extend the structure–activity relationships of OGA inhibitors, a series of 2-acetamido-2-deoxy-d-glucono-1,5-lactone sulfonylhydrazones was prepared from d-glucosamine. The synthetic sequence involved condensation of N-acetyl-3,4,6-tri-O-acetyl-d-glucosamine with arenesulfonylhydrazines, followed by MnO2 oxidation to the corresponding glucono-1,5-lactone sulfonylhydrazones. Removal of the O-acetyl protecting groups by NH3/MeOH furnished the test compounds. Evaluation of these compounds by enzyme kinetic methods against hOGA and hHexB revealed potent nanomolar competitive inhibition of both enzymes, with no significant selectivity towards either. The most efficient inhibitor of hOGA was 2-acetamido-2-deoxy-d-glucono-1,5-lactone 1-naphthalenesulfonylhydrazone (5f, Ki = 27 nM). This compound had a Ki of 6.8 nM towards hHexB. To assess the binding mode of these inhibitors to hOGA, computational studies (Prime protein–ligand refinement and QM/MM optimizations) were performed, which suggested the binding preference of the glucono-1,5-lactone sulfonylhydrazones in an s-cis conformation for all test compounds.
Ibuprofen, Flurbiprofen, Etoricoxib or Paracetamol Do Not Influence ACE2 Expression and Activity In Vitro or in Mice and Do Not Exacerbate In-Vitro SARS-CoV-2 Infection
Authors: de Bruin Natasja , Schneider Ann-Kathrin , Reus Philipp , Talmon Sonja , Ciesek Sandra , Bojkova Denisa , Cinatl Jindrich , Lodhi Imran , Charlesworth Bruce , Sinclair Simon , Pennick Graham , Laughey William F. , Gribbon Philip , Kannt Aimo , Schiffmann Susanne
Published: January 2022

SARS-CoV-2 uses the human cell surface protein angiotensin converting enzyme 2 (ACE2) as the receptor by which it gains access into lung and other tissue. Early in the pandemic, there was speculation that a number of commonly used medications—including ibuprofen and other non-steroidal anti-inflammatory drugs (NSAIDs)—have the potential to upregulate ACE2, thereby possibly facilitating viral entry and increasing the severity of COVID-19. We investigated the influence of the NSAIDS with a range of cyclooxygenase (COX)1 and COX2 selectivity (ibuprofen, flurbiprofen, etoricoxib) and paracetamol on the level of ACE2 mRNA/protein expression and activity as well as their influence on SARS-CoV-2 infection levels in a Caco-2 cell model. We also analysed the ACE2 mRNA/protein levels and activity in lung, heart and aorta in ibuprofen treated mice. The drugs had no effect on ACE2 mRNA/protein expression and activity in the Caco-2 cell model. There was no up-regulation of ACE2 mRNA/protein expression and activity in lung, heart and aorta tissue in ibuprofen-treated mice in comparison to untreated mice. Viral load was significantly reduced by both flurbiprofen and ibuprofen at high concentrations. Ibuprofen, flurbiprofen, etoricoxib and paracetamol demonstrated no effects on ACE2 expression or activity in vitro or in vivo. Higher concentrations of ibuprofen and flurbiprofen reduced SARS-CoV-2 replication in vitro.
Identification and Validation of Quantitative Trait Loci Mapping for Spike-Layer Uniformity in Wheat
Authors: Zhou Kunyu , Lin Yu , Jiang Xiaojun , Zhou Wanlin , Wu Fangkun , Li Caixia , Wei Yuming , Liu Yaxi
Published: January 2022

Spike-layer uniformity (SLU), the consistency of the spike distribution in the vertical space, is an important trait. It directly affects the yield potential and appearance. Revealing the genetic basis of SLU will provide new insights into wheat improvement. To map the SLU-related quantitative trait loci (QTL), 300 recombinant inbred lines (RILs) that were derived from a cross between H461 and Chinese Spring were used in this study. The RILs and parents were tested in fields from two continuous years from two different pilots. Phenotypic analysis showed that H461 was more consistent in the vertical spatial distribution of the spike layer than in Chinese Spring. Based on inclusive composite interval mapping, four QTL were identified for SLU. There were two major QTL on chromosomes 2BL and 2DL and two minor QTL on chromosomes 1BS and 2BL that were identified. The additive effects of QSlu.sicau-1B, Qslu.sicau-2B-2, and QSlu.sicau-2D were all from the parent, H461. The major QTL, QSlu.sicau-2B-2 and QSlu.sicau-2D, were detected in each of the conducted trials. Based on the best linear unbiased prediction values, the two loci explained 23.97% and 15.98% of the phenotypic variation, respectively. Compared with previous studies, the two major loci were potentially novel and the two minor loci were overlapped. Based on the kompetitive allele-specific PCR (KASP) marker, the genetic effects for QSlu.sicau-2B-2 were validated in an additional RIL population. The genetic effects ranged from 26.65% to 32.56%, with an average value of 30.40%. In addition, QSlu.sicau-2B-2 showed a significant (p < 0.01) and positive influence on the spike length, spikelet number, and thousand kernel weight. The identified QTL and the developed KASP marker will be helpful for fine-mapping these loci, finally contributing to wheat breeding programs in a marker-assisted selection way.
Functional Characterization of an In-Frame Deletion in the Basic Domain of the Retinal Transcription Factor ATOH7
Authors: Atac David , Mohn Lucas , Feil Silke , Maggi Kevin , Haenni Dominik , Seebauer Britta , Koller Samuel , Berger Wolfgang
Published: January 2022

Basic helix–loop–helix (bHLH) transcription factors are evolutionarily conserved and structurally similar proteins important in development. The temporospatial expression of atonal bHLH transcription factor 7 (ATOH7) directs the differentiation of retinal ganglion cells and mutations in the human gene lead to vitreoretinal and/or optic nerve abnormalities. Characterization of pathogenic ATOH7 mutations is needed to understand the functions of the conserved bHLH motif. The published ATOH7 in-frame deletion p.(Arg41_Arg48del) removes eight highly conserved amino acids in the basic domain. We functionally characterized the mutant protein by expressing V5-tagged ATOH7 constructs in human embryonic kidney 293T (HEK293T) cells for subsequent protein analyses, including Western blot, cycloheximide chase assays, Förster resonance energy transfer fluorescence lifetime imaging, enzyme-linked immunosorbent assays and dual-luciferase assays. Our results indicate that the in-frame deletion in the basic domain causes mislocalization of the protein, which can be rescued by a putative dimerization partner transcription factor 3 isoform E47 (E47), suggesting synergistic nuclear import. Furthermore, we observed (i) increased proteasomal degradation of the mutant protein, (ii) reduced protein heterodimerization, (iii) decreased DNA-binding and transcriptional activation of a reporter gene, as well as (iv) inhibited E47 activity. Altogether our observations suggest that the DNA-binding basic domain of ATOH7 has additional roles in regulating the nuclear import, dimerization, and protein stability.
Normal Retinotopy in Primary Visual Cortex in a Congenital Complete Unilateral Lesion of Lateral Geniculate Nucleus in Human: A Case Study
Authors: Bhat Akshatha , Kurzawski Jan W. , Anobile Giovanni , Tinelli Francesca , Biagi Laura , Morrone Maria Concetta
Published: January 2022

Impairment of the geniculostriate pathway results in scotomas in the corresponding part of the visual field. Here, we present a case of patient IB with left eye microphthalmia and with lesions in most of the left geniculostriate pathway, including the Lateral Geniculate Nucleus (LGN). Despite the severe lesions, the patient has a very narrow scotoma in the peripheral part of the lower-right-hemifield only (beyond 15° of eccentricity) and complete visual field representation in the primary visual cortex. Population receptive field mapping (pRF) of the patient’s visual field reveals orderly eccentricity maps together with contralateral activation in both hemispheres. With diffusion tractography, we revealed connections between superior colliculus (SC) and cortical structures in the hemisphere affected by the lesions, which could mediate the retinotopic reorganization at the cortical level. Our results indicate an astonishing case for the flexibility of the developing retinotopic maps where the contralateral thalamus receives fibers from both the nasal and temporal retinae.
N-6-Methyladenosine in Vasoactive microRNAs during Hypoxia; A Novel Role for METTL4
Authors: van den Homberg Daphne A. L. , van der Kwast Reginald V. C. T. , Quax Paul H. A. , Nossent A. Yaël
Published: January 2022

N-6-methyladenosine (m6A) is the most prevalent post-transcriptional RNA modification in eukaryotic cells. The modification is reversible and can be dynamically regulated by writer and eraser enzymes. Alteration in the levels of these enzymes can lead to changes in mRNA stability, alternative splicing or microRNA processing, depending on the m6A-binding proteins. Dynamic regulation of mRNA m6A methylation after ischemia and hypoxia influences mRNA stability, alternative splicing and translation, contributing to heart failure. In this study, we studied vasoactive microRNA m6A methylation in fibroblasts and examined the effect of hypoxia on microRNAs methylation using m6A immunoprecipitation. Of the 19 microRNAs investigated, at least 16 contained m6A in both primary human fibroblasts and a human fibroblast cell line, suggesting vasoactive microRNAs are commonly m6A methylated in fibroblasts. More importantly, we found that mature microRNA m6A levels increased upon subjecting cells to hypoxia. By silencing different m6A writer and eraser enzymes followed by m6A immunoprecipitation, we identified METTL4, an snRNA m6A methyltransferase, to be predominantly responsible for the increase in m6A modification. Moreover, by using m6A-methylated microRNA mimics, we found that microRNA m6A directly affects downstream target mRNA repression efficacy. Our findings highlight the regulatory potential of the emerging field of microRNA modifications.
Cellular and Gene Expression Response to the Combination of Genistein and Kaempferol in the Treatment of Mucopolysaccharidosis Type I
Authors: Węsierska Magdalena , Kloska Anna , Medina Diego L. , Jakóbkiewicz-Banecka Joanna , Gabig-Cimińska Magdalena , Radzińska Marta , Moskot Marta , Malinowska Marcelina
Published: January 2022

Flavonoids are investigated as therapeutics for mucopolysaccharidosis, a metabolic disorder with impaired glycosaminoglycan degradation. Here we determined the effects of genistein and kaempferol, used alone or in combination, on cellular response and gene expression in a mucopolysaccharidosis type I model. We assessed the cell cycle, viability, proliferation, subcellular localization of the translocation factor EB (TFEB), number and distribution of lysosomes, and glycosaminoglycan synthesis after exposure to flavonoids. Global gene expression was analysed using DNA microarray and quantitative PCR. The type and degree of flavonoid interaction were determined based on the combination and dose reduction indexes. The combination of both flavonoids synergistically inhibits glycosaminoglycan synthesis, modulates TFEB localization, lysosomal number, and distribution. Genistein and kaempferol in a 1:1 ratio regulate the expression of 52% of glycosaminoglycan metabolism genes. Flavonoids show synergy, additivity, or slight antagonism in all analysed parameters, and the type of interaction depends on the concentration and component ratios. With the simultaneous use of genistein and kaempferol in a ratio of 4:1, even a 10-fold reduction in the concentration of kaempferol is possible. Flavonoid mixtures, used as the treatment of mucopolysaccharidosis, are effective in reducing glycosaminoglycan production and storage and show a slight cytotoxic effect compared to single-flavonoid usage.
Root GS and NADH-GDH Play Important Roles in Enhancing the Ammonium Tolerance in Three Bedding Plants
Authors: Song Jinnan , Yang Jingli , Jeong Byoung Ryong
Published: January 2022

Ammonium is a paradoxical nutrient because it is more metabolically efficient than nitrate, but also causes plant stresses in excess, i.e., ammonium toxicity. Current knowledge indicates that ammonium tolerance is species-specific and related to the ammonium assimilation enzyme activities. However, the mechanisms underlying the ammonium tolerance in bedding plants remain to be elucidated. The study described herein explores the primary traits contributing to the ammonium tolerance in three bedding plants. Three NH4+:NO3− ratios (0:100, 50:50, 100:0) were supplied to salvia, petunia, and ageratum. We determined that they possessed distinct ammonium tolerances: salvia and petunia were, respectively, extremely sensitive and moderately sensitive to high NH4+ concentrations, whereas ageratum was tolerant to NH4+, as characterized by the responses of the shoot and root growth, photosynthetic capacity, and nitrogen (amino acid and soluble protein)-carbohydrate (starch) distributions. An analysis of the major nitrogen assimilation enzymes showed that the root GS (glutamine synthetase) and NADH-GDH (glutamate dehydrogenase) activities in ageratum exhibited a dose-response relationship (reinforced by 25.24% and 6.64%, respectively) as the NH4+ level was raised from 50% to 100%; but both enzyme activities were significantly diminished in salvia. Besides, negligible changes of GS activities monitored in leaves revealed that only the root GS and NADH-GDH underpin the ammonium tolerances of the three bedding plants.
Analogs of the Heat Shock Protein 70 Inhibitor MKT-077 Suppress Medullary Thyroid Carcinoma Cells
Authors: Hong Seung-Keun , Starenki Dmytro , Johnson Oleta T. , Gestwicki Jason E. , Park Jong-In
Published: January 2022

Medullary thyroid carcinoma (MTC) is a neuroendocrine tumor mainly caused by mutations in the RET proto-oncogene. We previously demonstrated that depletion of the mitochondrial molecular chaperone, mortalin, can effectively suppress human MTC cells in culture and in mouse xenografts, by disrupting mitochondrial bioenergetics and subsequently inducing apoptosis and RET downregulation. Similar effects were induced by MKT-077, a water-soluble rhodocyanine dye analog known to inhibit mortalin, but with notable toxicity in animals. These observations led us to evaluate recently developed MKT-077 analogs that exhibited higher selectivity to HSP70 proteins and improved bioavailability. We validated the MTC cell-suppressive effects of mortalin depletion in three-dimensional cultures of the human MTC lines, TT, and MZ-CRC-1, and then evaluated different MKT-077 analogs in two- and three-dimensional cell cultures, to show that the MKT-077 analogs, JG-98 and JG-194, effectively and consistently inhibited propagation of TT and MZ-CRC-1 cells in these cultures. Of note, these compounds also effectively suppressed the viability of TT and MZ-CRC-1 progenies resistant to vandetanib and cabozantinib. Moreover, JG-231, an analog with improved microsomal stability, consistently suppressed TT and MZ-CRC-1 xenografts in mice. These data suggest that mortalin inhibition may have therapeutic potential for MTC.
Emerging Insights on the Diverse Roles of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in Chronic Liver Diseases: Cholesterol Metabolism and Beyond
Authors: Grewal Thomas , Buechler Christa
Published: January 2022

Chronic liver diseases are commonly associated with dysregulated cholesterol metabolism. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease of the proprotein convertase family that is mainly synthetized and secreted by the liver, and represents one of the key regulators of circulating low-density lipoprotein (LDL) cholesterol levels. Its ability to bind and induce LDL-receptor degradation, in particular in the liver, increases circulating LDL-cholesterol levels in the blood. Hence, inhibition of PCSK9 has become a very potent tool for the treatment of hypercholesterolemia. Besides PCSK9 limiting entry of LDL-derived cholesterol, affecting multiple cholesterol-related functions in cells, more recent studies have associated PCSK9 with various other cellular processes, including inflammation, fatty acid metabolism, cancerogenesis and visceral adiposity. It is increasingly becoming evident that additional roles for PCSK9 beyond cholesterol homeostasis are crucial for liver physiology in health and disease, often contributing to pathophysiology. This review will summarize studies analyzing circulating and hepatic PCSK9 levels in patients with chronic liver diseases. The factors affecting PCSK9 levels in the circulation and in hepatocytes, clinically relevant studies and the pathophysiological role of PCSK9 in chronic liver injury are discussed.
Pericyte and Vascular Smooth Muscle Death in Diabetic Retinopathy Involves Autophagy
Authors: Gardiner Tom A. , Stitt Alan W.
Published: January 2022

Diabetic retinopathy (DR) is the most common complication of diabetes and a major cause of vision loss worldwide. The premature death of the microvascular mural cells represents both a pathological hallmark of vasodegeneration in DR and a basis for therapeutic intervention to halt progression to the sight-threatening stages. Recent studies suggest that retinal microvascular mural cells, classed as pericytes in the capillaries and vascular smooth muscle cells in the larger vessels (VSMC), may undergo autophagy-dependent cell death during DR. The present investigation was undertaken to assess electron microscopic evidence for involvement of autophagy in mediation of cell death in the mural cells of the retinal vasculature, in eyes from human diabetic donors and diabetic dogs. All specimens examined showed widespread evidence of autophagosomes in processes of viable pericytes and VSMCs, and the membranous remnants of excessive autophagic activity in their “ghost cell” remnants within the vascular walls. Autophagy was termed “excessive” when it occupied the greater part of the cytoplasm in mural cell processes. This was notable in specimens from short-term diabetic donors with no evidence of basement-membrane thickening or mural cell loss, in which regions of mural cell cytoplasm filled with autophagic bodies appeared to be undergoing cytoplasmic cleavage. No equivalent evidence of autophagy was detected in the adjacent endothelial cells of the retinal vessels. We conclude that increased autophagy in the retinal pericytes and VSMCs is linked to the diabetic milieu, and over time may also act as a trigger for mural cell loss and progressive vasodegeneration.
Optimization of Food Industry Production Using the Monte Carlo Simulation Method: A Case Study of a Meat Processing Plant
Authors: Koroteev Mikhail , Romanova Ekaterina , Korovin Dmitriy , Shevtsov Vasiliy , Feklin Vadim , Nikitin Petr , Makrushin Sergey , Bublikov Konstantin V.
Published: January 2022

The problem evaluated in this study is related to the optimization of a budget of an industrial enterprise using simulation methods of the production process. Our goal is to offer a universal and straightforward methodology for simulating a production budget at any level of complexity by presenting it in a specific form. The calculation of such production schemes, in most enterprises, is currently done manually, which significantly limits the possibilities for optimization. This article proposes a model based on the Monte Carlo method to automate the budgeting process. The application of this model is described using an example of a typical meat processing enterprise. Approbation of the model showed its high applicability and the ability to transform the process of making management decisions and the potential to increase the profits of the enterprise, which is unattainable using other methods. As a result of the study, we present a methodology for modeling industrial production that can significantly speed up the formation and optimization of an enterprise’s budget. In our demonstration case, the profit increased by over 30 percentage points.
Transferrable Framework Based on Knowledge Graphs for Generating Explainable Results in Domain-Specific, Intelligent Information Retrieval
Authors: Abu-Rasheed Hasan , Weber Christian , Zenkert Johannes , Dornhöfer Mareike , Fathi Madjid
Published: January 2022

In modern industrial systems, collected textual data accumulates over time, offering an important source of information for enhancing present and future industrial practices. Although many AI-based solutions have been developed in the literature for a domain-specific information retrieval (IR) from this data, the explainability of these systems was rarely investigated in such domain-specific environments. In addition to considering the domain requirements within an explainable intelligent IR, transferring the explainable IR algorithm to other domains remains an open-ended challenge. This is due to the high costs, which are associated with intensive customization and required knowledge modelling, when developing new explainable solutions for each industrial domain. In this article, we present a transferable framework for generating domain-specific explanations for intelligent IR systems. The aim of our work is to provide a comprehensive approach for constructing explainable IR and recommendation algorithms, which are capable of adopting to domain requirements and are usable in multiple domains at the same time. Our method utilizes knowledge graphs (KG) for modeling the domain knowledge. The KG provides a solid foundation for developing intelligent IR solutions. Utilizing the same KG, we develop graph-based components for generating textual and visual explanations of the retrieved information, taking into account the domain requirements and supporting the transferability to other domain-specific environments, through the structured approach. The use of the KG resulted in minimum-to-zero adjustments when creating explanations for multiple intelligent IR algorithms in multiple domains. We test our method within two different use cases, a semiconductor manufacturing centered use case and a job-to-applicant matching one. Our quantitative results show a high capability of our approach to generate high-level explanations for the end users. In addition, the developed explanation components were highly adaptable to both industrial domains without sacrificing the overall accuracy of the intelligent IR algorithm. Furthermore, a qualitative user-study was conducted. We recorded a high level of acceptance from the users, who reported an enhanced overall experience with the explainable IR system.
Will the Internet of Things Be Perovskite Powered? Energy Yield Measurement and Real-World Performance of Perovskite Solar Cells in Ambient Light Conditions
Authors: Thomas Suzanne K. , Pockett Adam , Seunarine Krishna , Spence Michael , Raptis Dimitrios , Meroni Simone , Watson Trystan , Jones Matt , Carnie Matthew J.
Published: January 2022

The number of interconnected devices, often referred to as the Internet of Things (IoT), is increasing at a considerable rate. It is inevitable therefore that so too will the energy demand. IoT describes a range of technologies such as sensors, software, smart meters, wearable devices, and communication beacons for the purpose of connecting and exchanging data with other devices and systems over the internet. Often not located near a mains supply power source, these devices may be reliant on primary battery cells. To avoid the need to periodically replace these batteries, it makes sense to integrate the technologies with a photovoltaic (PV) cell to harvest ambient light, so that the technologies can be said to be self-powered. Perovskite solar cells have proven extremely efficient in low-light conditions but in the absence of ambient and low-light testing standards, or even a consensus on what is defined by “ambient light”, it is difficult to estimate the energy yield of a given PV technology in a given scenario. Ambient light harvesting is complex, subject to spectral considerations, and whether the light source is directly incident on the PV cell. Here, we present a realistic scenario-driven method for measuring the energy yield for a given PV technology in various situations in which an IoT device may be found. Furthermore, we show that laboratory-built p-i-n perovskite devices, for many scenarios, produce energy yields close to that of commercial GaAs solar cells. Finally, we demonstrate an IoT device, powered by a mesoporous carbon perovskite solar module and supercapacitor, and operating through several day–night cycles.