bluuebunny/crossref_metadata_2025 huggingface dataset:
Global Analysis of the Human RNA Degradome Reveals Widespread Decapped and Endonucleolytic Cleaved Transcripts
Authors: Won Jung-Im , Shin JaeMoon , Park So Young , Yoon JeeHee , Jeong Dong-Hoon
Published: September 2020

RNA decay is an important regulatory mechanism for gene expression at the posttranscriptional level. Although the main pathways and major enzymes that facilitate this process are well defined, global analysis of RNA turnover remains under-investigated. Recent advances in the application of next-generation sequencing technology enable its use in order to examine various RNA decay patterns at the genome-wide scale. In this study, we investigated human RNA decay patterns using parallel analysis of RNA end-sequencing (PARE-seq) data from XRN1-knockdown HeLa cell lines, followed by a comparison of steady state and degraded mRNA levels from RNA-seq and PARE-seq data, respectively. The results revealed 1103 and 1347 transcripts classified as stable and unstable candidates, respectively. Of the unstable candidates, we found that a subset of the replication-dependent histone transcripts was polyadenylated and rapidly degraded. Additionally, we identified 380 endonucleolytically cleaved candidates by analyzing the most abundant PARE sequence on a transcript. Of these, 41.4% of genes were classified as unstable genes, which implied that their endonucleolytic cleavage might affect their mRNA stability. Furthermore, we identified 1877 decapped candidates, including HSP90B1 and SWI5, having the most abundant PARE sequences at the 5′-end positions of the transcripts. These results provide a useful resource for further analysis of RNA decay patterns in human cells.
Neuroinflammatory Mechanisms in Ischemic Stroke: Focus on Cardioembolic Stroke, Background, and Therapeutic Approaches
Authors: Maida Carlo Domenico , Norrito Rosario Luca , Daidone Mario , Tuttolomondo Antonino , Pinto Antonio
Published: September 2020

One of the most important causes of neurological morbidity and mortality in the world is ischemic stroke. It can be a result of multiple events such as embolism with a cardiac origin, occlusion of small vessels in the brain, and atherosclerosis affecting the cerebral circulation. Increasing evidence shows the intricate function played by the immune system in the pathophysiological variations that take place after cerebral ischemic injury. Following the ischemic cerebral harm, we can observe consequent neuroinflammation that causes additional damage provoking the death of the cells; on the other hand, it also plays a beneficial role in stimulating remedial action. Immune mediators are the origin of signals with a proinflammatory position that can boost the cells in the brain and promote the penetration of numerous inflammatory cytotypes (various subtypes of T cells, monocytes/macrophages, neutrophils, and different inflammatory cells) within the area affected by ischemia; this process is responsible for further ischemic damage of the brain. This inflammatory process seems to involve both the cerebral tissue and the whole organism in cardioembolic stroke, the stroke subtype that is associated with more severe brain damage and a consequent worse outcome (more disability, higher mortality). In this review, the authors want to present an overview of the present learning of the mechanisms of inflammation that takes place in the cerebral tissue and the role of the immune system involved in ischemic stroke, focusing on cardioembolic stroke and its potential treatment strategies.
Novel ANO1 Inhibitor from Mallotus apelta Extract Exerts Anticancer Activity through Downregulation of ANO1
Authors: Seo Yohan , Anh Nguyen Hoang , Heo Yunkyung , Park So-Hyeon , Kiem Phan Van , Lee Yechan , Yen Duong Thi Hai , Jo Sungwoo , Jeon Dongkyu , Tai Bui Huu , Nam Nguyen Hoai , Minh Chau Van , Kim Seung Hyun , Nhiem Nguyen Xuan , Namkung Wan
Published: September 2020

Anoctamin1 (ANO1), a calcium-activated chloride channel, is frequently overexpressed in several cancers, including human prostate cancer and oral squamous cell carcinomas. ANO1 plays a critical role in tumor growth and maintenance of these cancers. In this study, we have isolated two new compounds (1 and 2) and four known compounds (3–6) from Mallotus apelta. These compounds were evaluated for their inhibitory effects on ANO1 channel activity and their cytotoxic effects on PC-3 prostate cancer cells. Interestingly, compounds 1 and 2 significantly reduced both ANO1 channel activity and cell viability. Electrophysiological study revealed that compound 2 (Ani-D2) is a potent and selective ANO1 inhibitor, with an IC50 value of 2.64 μM. Ani-D2 had minimal effect on cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel activity and intracellular calcium signaling. Notably, Ani-D2 significantly reduced ANO1 protein expression levels and cell viability in an ANO1-dependent manner in PC-3 and oral squamous cell carcinoma CAL-27 cells. In addition, Ani-D2 strongly reduced cell migration and induced activation of caspase-3 and cleavage of PARP in PC-3 and CAL-27 cells. This study revealed that a novel ANO1 inhibitor, Ani-D2, has therapeutic potential for the treatment of several cancers that overexpress ANO1, such as prostate cancer and oral squamous cell carcinoma.
Modeling Immune Checkpoint Inhibitor Efficacy in Syngeneic Mouse Tumors in an Ex Vivo Immuno-Oncology Dynamic Environment
Authors: Doty Daniel T. , Schueler Julia , Mott Vienna L. , Bryan Cassie M. , Moore Nathan F. , Ho John C. , Borenstein Jeffrey T.
Published: September 2020

The immune checkpoint blockade represents a revolution in cancer therapy, with the potential to increase survival for many patients for whom current treatments are not effective. However, response rates to current immune checkpoint inhibitors vary widely between patients and different types of cancer, and the mechanisms underlying these varied responses are poorly understood. Insights into the antitumor activities of checkpoint inhibitors are often obtained using syngeneic mouse models, which provide an in vivo preclinical basis for predicting efficacy in human clinical trials. Efforts to establish in vitro syngeneic mouse equivalents, which could increase throughput and permit real-time evaluation of lymphocyte infiltration and tumor killing, have been hampered by difficulties in recapitulating the tumor microenvironment in laboratory systems. Here, we describe a multiplex in vitro system that overcomes many of the deficiencies seen in current static histocultures, which we applied to the evaluation of checkpoint blockade in tumors derived from syngeneic mouse models. Our system enables both precision-controlled perfusion across biopsied tumor fragments and the introduction of checkpoint-inhibited tumor-infiltrating lymphocytes in a single experiment. Through real-time high-resolution confocal imaging and analytics, we demonstrated excellent correlations between in vivo syngeneic mouse and in vitro tumor biopsy responses to checkpoint inhibitors, suggesting the use of this platform for higher throughput evaluation of checkpoint efficacy as a tool for drug development.
Evaluation of Suppressed Mite Reproduction (SMR) Reveals Potential for Varroa Resistance in European Honey Bees (Apis mellifera L.)
Authors: Mondet Fanny , Parejo Melanie , Meixner Marina D. , Costa Cecilia , Kryger Per , Andonov Sreten , Servin Bertrand , Basso Benjamin , Bieńkowska Małgorzata , Bigio Gianluigi , Căuia Eliza , Cebotari Valentina , Dahle Bjorn , Dražić Marica Maja , Hatjina Fani , Kovačić Marin , Kretavicius Justinas , Lima Ana S. , Panasiuk Beata , Pinto M. Alice , Uzunov Aleksandar , Wilde Jerzy , Büchler Ralph
Published: September 2020

In the fight against the Varroa destructor mite, selective breeding of honey bee (Apis mellifera L.) populations that are resistant to the parasitic mite stands as a sustainable solution. Selection initiatives indicate that using the suppressed mite reproduction (SMR) trait as a selection criterion is a suitable tool to breed such resistant bee populations. We conducted a large European experiment to evaluate the SMR trait in different populations of honey bees spread over 13 different countries, and representing different honey bee genotypes with their local mite parasites. The first goal was to standardize and validate the SMR evaluation method, and then to compare the SMR trait between the different populations. Simulation results indicate that it is necessary to examine at least 35 single-infested cells to reliably estimate the SMR score of any given colony. Several colonies from our dataset display high SMR scores indicating that this trait is present within the European honey bee populations. The trait is highly variable between colonies and some countries, but no major differences could be identified between countries for a given genotype, or between genotypes in different countries. This study shows the potential to increase selective breeding efforts of V. destructor resistant populations.
Phytochemical Screening and Bioactivity of Ludwigia spp. in the Control of Plutella xylostella (Lepidoptera: Plutellidae)
Authors: Ferreira Eliana Aparecida , de Souza Silvana Aparecida , Domingues Alberto , Da Silva Matheus Moreno Mareco , Padial Isabella Maria Pompeu Monteiro , de Carvalho Emerson Machado , Cardoso Claudia Andrea Lima , da Silva Sandra Verza , Mussury Rosilda Mara
Published: September 2020

We tested the bioactivity of aqueous extracts of Ludwigia spp. (Myrtales: Onagraceae) on the biological cycle of Plutella xylostella. We assessed the duration of and viability during the larval, pupal and adult phases, as well as the influence of the extracts on the fecundity and hatching of P. xylostella eggs. Subsequently, we phytochemically screened the extracts. The extracts of L. tomentosa and L. longifolia reduced the pupal weight instead of prolonging the larval stage of P. xylostella. The L. tomentosa effect caused higher larval mortality and reduced the fecundity and hatching of P. xylostella eggs, and L. sericea reduced the egg survival. The phenolic compounds—flavonoids, condensed tannins and alkaloids—were more abundant in L. nervosa, L. tomentosa, L. sericea and L. longifolia. The L. tomentosa, L. longifolia and L. sericea extracts were bioactive, and these species showed the best results regarding their ability to control P. xylostella populations, because these plants produce substances able to inhibit food consumption and interfere with the morphological and physiological transformations of the offspring and the oviposition of adults.
Impact of 2.45 GHz Microwave Irradiation on the Fruit Fly, Drosophila melanogaster
Authors: Yanagawa Aya , Tomaru Masatoshi , Kajiwara Atsushi , Nakajima Hiroki , Quemener Elie Desmond-Le , Steyer Jean-Philippe , Mitani Tomohiko
Published: September 2020

The physiological and behavioral influences of 2.45 GHz microwaves on Drosophila melanogaster were examined. Standing waves transitioned into heat energy effectively when passing through the insect body. On the contrary, travelling waves did not transit into heat energy in the insect body. This indicated that there was no concern regarding the thermal effects of microwave irradiation for levels of daily usage. However, we detected genotoxicity and behavioral alterations associated with travelling wave irradiation, which can be attributed to the non-thermal effects of the waves. Electron spin resonance (ESR) revealed that fruit flies possessed paramagnetic substances in the body such as Fe3+, Cu2+, Mn2+, and organic radicals. The temperature dependent intensities of these paramagnetic substances indicated that females possessed more of the components susceptible to electromagnetic waves than males, and the behavioral tests supported the differences between the sexes.
Susceptibility of the Cigarette Beetle Lasioderma serricorne (Fabricius) to Phosphine, Ethyl Formate and Their Combination, and the Sorption and Desorption of Fumigants on Cured Tobacco Leaves
Authors: Kim Bong Su , Shin Eun-Mi , Park Young Ju , Yang Jeong Oh
Published: September 2020

The susceptibility of Lasioderma serricorne to phosphine (PH3), ethyl formate (EF) and their combination (PH3 + EF) was evaluated in this study. Eggs, larvae, pupae and adults were subjected to treatment with fumigants to determine the 90% lethal concentration time (LCt90) values. Treatment with PH3 for 20 h resulted in LCt90 values of 1.15, 1.39, 14.97 and 1.78 mg h/L while treatment with EF resulted in values of 157.96, 187.75, 126.06 and 83.10 mg h/L, respectively. By contrast, the combination of PH3 + EF resulted in LCt90 values of 36.05, 44.41, 187.17 and 35.12 mg h/L after 4 h. These results show that, through treatment with PH3 + EF, control can be achieved at lower concentrations than for treatment with EF alone and at lower exposure times than for treatment with PH3 alone. The sorption rates of the fumigants on cured tobacco leaves were determined for filling ratios of 2.5%, 5.0% and 10.0% (w/v). Cured tobacco leaves were treated with either 2 mg/L PH3, 114 mg/L EF or 0.5 mg/L PH3 + 109 mg/L EF. Treatment with PH3 showed sorption rates of 0.0%, 7.1% and 14.3%. EF, however, showed higher sorption rates of 64.9%, 68.5% and 75.5%, respectively, for the indicated filling ratios. When PH3 and EF were combined, the sorption rate of PH3 was 0.0%, while the sorption rates of EF were lower (9.1%, 12.0% and 23.2%) than treatment with only EF. EF required a ventilation time of longer than 22 h to desorb from cured tobacco leaves. Therefore, PH3 + EF can effectively control L. serricorne in cured tobacco leaves, with sufficient ventilation time required after treatment for the safety of workers.
Is Virtual Reality Effective for Balance Recovery in Patients with Spinal Cord Injury? A Systematic Review and Meta-Analysis
Authors: Miguel-Rubio Amaranta De , Rubio M. Dolores , Salazar Alejandro , Moral-Munoz Jose A. , Requena Francisco , Camacho Rocio , Lucena-Anton David
Published: September 2020

Virtual reality (VR) is an emerging tool used in the neurological rehabilitation of patients with spinal cord injury (SCI), focused on recovering balance, mobility, and motor function, among other functional outcomes. The main objective of this study was to analyze the effectiveness of VR systems to recover balance in patients with SCI. The literature search was performed between October and December 2019 in the following databases: Embase, Web of Science, CINAHL, Scopus, Medline, Physiotherapy Evidence Database (PEDro), PubMed, and the Cochrane Central Register of Controlled Trials. The methodological quality of each study was assessed using the Spinal Cord Injury Rehabilitation Evidence (SCIRE) system and the PEDro scale, while the risk of bias was analyzed by the Cochrane Collaboration’s tool. A total of 12 studies, involving 188 participants, were included in the systematic review, of which two were included in the meta-analysis. Statistical analysis showed favorable results for balance measured by the modified Functional Reach Test (standardized mean difference (SMD) = 3.42; 95% confidence interval: 2.54 to 4.29) and by the t-shirt test (SMD= −2.29; 95% confidence interval: −3.00 to −1.59). The results showed that VR interventions provided potential benefits, in addition to conventional physical therapy, to recover balance in patients with SCI.
Novel Approaches to Improve Myeloma Cell Killing by Monoclonal Antibodies
Authors: Storti Paola , Costa Federica , Marchica Valentina , Burroughs-Garcia Jessica , dalla Palma Benedetta , Toscani Denise , Eufemiese Rosa Alba , Giuliani Nicola
Published: September 2020

The monoclonal antibodies (mAbs) have significantly changed the treatment of multiple myeloma (MM) patients. However, despite their introduction, MM remains an incurable disease. The mAbs currently used for MM treatment were developed with different mechanisms of action able to target antigens, such as cluster of differentiation 38 (CD38) and SLAM family member 7 (SLAMF7) expressed by both, MM cells and the immune microenvironment cells. In this review, we focused on the mechanisms of action of the main mAbs approved for the therapy of MM, and on the possible novel approaches to improve MM cell killing by mAbs. Actually, the combination of anti-CD38 or anti-SLAMF7 mAbs with the immunomodulatory drugs significantly improved the clinical effect in MM patients. On the other hand, pre-clinical evidence indicates that different approaches may increase the efficacy of mAbs. The use of trans-retinoic acid, the cyclophosphamide or the combination of anti-CD47 and anti-CD137 mAbs have given the rationale to design these types of combinations therapies in MM patients in the future. In conclusion, a better understanding of the mechanism of action of the mAbs will allow us to develop novel therapeutic approaches to improve their response rate and to overcome their resistance in MM patients.
Ascitic Interleukin 6 Is Associated with Poor Outcome and Spontaneous Bacterial Peritonitis: A Validation in Critically Ill Patients with Decompensated Cirrhosis
Authors: Mayr Ulrich , Lukas Marina , Elnegouly Mayada , Vogt Christine , Bauer Ulrike , Ulrich Joerg , Schmid Roland M. , Huber Wolfgang , Lahmer Tobias
Published: September 2020

Decompensated cirrhosis predisposes to infectious diseases and acute-on-chronic liver failure (ACLF) in critically ill patients. Infections like spontaneous bacterial peritonitis (SBP) are frequently associated with multi-organ failure and increased mortality. Consequently, reliable predictors of outcome and early diagnostic markers of infection are needed to improve individualized therapy. This study evaluates the prognostic role of ascitic interleukin 6 in 64 patients with cirrhosis admitted to our intensive care unit (ICU). In addition, we analysed the diagnostic ability of ascitic interleukin 6 in a subgroup of 19 patients with SBP. Baseline ascitic interleukin 6 performed well in predicting 3-month mortality in patients with decompensated cirrhosis (area under curve (AUC) = 0.802), as well as in patients fulfilling ACLF-criteria (AUC = 0.807). Ascitic interleukin 6 showed a moderate prognostic advantage compared with common clinical scores and proinflammatory parameters. Moreover, ascitic interleukin 6 had a sufficient diagnostic ability to detect SBP (AUC = 0.901) and was well correlated with ascitic polymorphonuclear neutrophils in SBP (p = 0.002). Interestingly, ascitic interleukin 6 revealed a high predictive value to rule out apparent infections on admission to ICU (AUC = 0.904) and to identify patients with “culture-positive SBP” (AUC = 0.856). Ascitic interleukin 6 is an easily-applicable proinflammatory biomarker with high prognostic and diagnostic relevance in critically ill patients with liver cirrhosis.
Fructosamine-3-Kinase as a Potential Treatment Option for Age-Related Macular Degeneration
Authors: De Bruyne Sander , Van den Broecke Caroline , Vrielinck Henk , Khelifi Samira , De Wever Olivier , Bracke Ken , Huizing Manon , Boston Nezahat , Himpe Jonas , Speeckaert Marijn , Vral Anne , Van Dorpe Jo , Van Aken Elisabeth , Delanghe Joris R.
Published: September 2020

Age-related macular degeneration is the leading cause of blindness in the developed world. Since advanced glycation end products (AGEs) are implicated in the pathogenesis of AMD through various lines of evidence, we investigated the potential of fructosamine-3-kinase (FN3K) in the disruption of retinal AGEs, drusenoid material and drusenoid lesions in patients with AMD. AGE-type autofluorescence was measured to evaluate the effects of FN3K on glycolaldehyde-induced AGE-modified neural porcine retinas and unmodified human neural retinas. Eye pairs from cigarette-smoke- and air-exposed mice were treated and evaluated histologically. Automated optical image analysis of human tissue sections was performed to compare control- and FN3K-treated drusen and near-infrared (NIR) microspectroscopy was performed to examine biochemical differences. Optical coherence tomography (OCT) was used to evaluate the effect of FN3K on drusenoid deposits after treatment of post-mortem human eyes. FN3K treatment provoked a significant decrease (41%) of AGE-related autofluorescence in the AGE-modified porcine retinas. Furthermore, treatment of human neural retinas resulted in significant decreases of autofluorescence (−24%). FN3K-treated murine eyes showed less drusenoid material. Pairwise comparison of drusen on tissue sections revealed significant changes in color intensity after FN3K treatment. NIR microspectroscopy uncovered clear spectral differences in drusenoid material (Bruch’s membrane) and drusen after FN3K treatment. Ex vivo treatment strongly reduced size of subretinal drusenoid lesions on OCT imaging (up to 83%). In conclusion, our study demonstrated for the first time a potential role of FN3K in the disruption of AGE-related retinal autofluorescence, drusenoid material and drusenoid lesions in patients with AMD.
Measuring Temperature in Coral Reef Environments: Experience, Lessons, and Results from Palau
Authors: Colin Patrick L. , Johnston T. M. Shaun
Published: September 2020

Sea surface temperature, determined remotely by satellite (SSST), measures only the thin “skin” of the ocean but is widely used to quantify the thermal regimes on coral reefs across the globe. In situ measurements of temperature complements global satellite sea surface temperature with more accurate measurements at specific locations/depths on reefs and more detailed data. In 1999, an in situ temperature-monitoring network was started in the Republic of Palau after the 1998 coral bleaching event. Over two decades the network has grown to 70+ stations and 150+ instruments covering a 700 km wide geographic swath of the western Pacific dominated by multiple oceanic currents. The specific instruments used, depths, sampling intervals, precision, and accuracy are considered with two goals: to provide comprehensive general coverage to inform global considerations of temperature patterns/changes and to document the thermal dynamics of many specific habitats found within a highly diverse tropical marine location. Short-term in situ temperature monitoring may not capture broad patterns, particularly with regard to El Niño/La Niña cycles that produce extreme differences. Sampling over two decades has documented large T signals often invisible to SSST from (1) internal waves on time scales of minutes to hours, (2) El Niño on time scales of weeks to years, and (3) decadal-scale trends of +0.2 °C per decade. Network data have been used to create a regression model with SSST and sea surface height (SSH) capable of predicting depth-varying thermal stress. The large temporal, horizontal, and vertical variability noted by the network has further implications for thermal stress on the reef. There is a dearth of definitive thermal information for most coral reef habitats, which undermines the ability to interpret biological events from the most basic physical perspective.
Deep Learning in Unmanned Surface Vehicles Collision-Avoidance Pattern Based on AIS Big Data with Double GRU-RNN
Authors: Shi Jia-hui , Liu Zheng-jiang
Published: September 2020

There is a collection of a large amount of automatic identification system (AIS) data that contains ship encounter information, but mining the collision avoidance knowledge from AIS big data and carrying out effective machine learning is a difficult problem in current maritime field. Herein, first the Douglas–Peucker (DP) algorithm was used to preprocess the AIS data. Then, based on the ship domain the risk of collision was identified. Finally, a double-gated recurrent unit neural network (GRU-RNN) was constructed to learn unmanned surface vehicle (USV) collision avoidance decision from the extracted data of successful encounters of ships. The double GRU-RNN was trained on the 2015 Tianjin Port AIS dataset to realize the effective learning of ship encounter data. The results indicated that the double GRU-RNN could effectively learn the collision avoidance pattern hidden in AIS big data, and generate corresponding ship collision-avoidance decisions for different maritime navigation states. This study contributes significantly to the increased efficiency and safety of sea operations. The proposed method could be potentially applied to USV technology and intelligence collision avoidance.
Identifying Potential Connectivity for an Urban Population of Rattlesnakes (Sistrurus catenatus) in a Canadian Park System
Authors: Choquette Jonathan D. , Macpherson Matthew R. , Corry Robert C.
Published: September 2020

In the face of ongoing habitat loss and fragmentation, maintaining an adequate level of landscape connectivity is needed to both encourage dispersal between habitat patches and to reduce the extinction risk of fragmented wildlife populations. In a developing region of southwestern Ontario, Canada, a declining population of Eastern Massasauga rattlesnakes (Sistrurus catenatus) persists in fragmented remnants of tallgrass prairie in an urban park system. The goal of this study was to identify potential connectivity pathways between habitat patches for this species by using a GIS least-cost permeability swath model, and to evaluate the outputs with snake road mortality data. Results identified seven pathways between five core habitat blocks, a subset of which were validated with aerial imagery and mortality data. Four high-ranking pathways intersected roads through or near road mortality hotspots. This research will guide conservation interventions aimed at recovering endangered reptiles in a globally rare ecosystem, and will inform the use of permeability swaths for the identification of locations most suitable for connectivity interventions in dynamic, urbanizing landscapes.
Transformations in the Agricultural and Scenic Landscapes in the Northwest of the Region of Murcia (Spain): Moving towards Long Awaited (Un)Sustainability
Authors: García-Marín Ramón , Espejo-Marín Cayetano , Giménez-García Rubén , Ruiz-Álvarez Víctor
Published: September 2020

Since the middle of the 20th century, irrigation in the southeast of Spain has displayed significant productive growth based on the intensive use of the scarce water resources in the area and the contribution of river flows from the hydrographic basin of the Tagus River to the hydrographic basin of the Segura River. Despite high levels of efficiency in the water use from the new irrigation systems, the water deficit has only intensified in recent years. The most dynamically irrigated areas (Campo de Cartagena, Valle del Guadalentín, Vega Alta del Segura and the southern coast of the Region of Murcia), were faced with a complex and trying future, resulting in numerous companies (agribusinesses) relocating to lease and acquire land in the northwest of Murcia to develop their intensive crops. The general objective of this article lies in the analysis of widespread landscape dynamics, and of agricultural dynamics in particular, in the rural environment of the northwest Region of Murcia (Spain). For this, an exhaustive analysis of the land cover and use transformations is carried out for the periods of time 1990–2000–2012–2018. The data studied come from the Corine Land Cover (CLC) project, carried out by the European Environment Agency (EEA). These spatial data are treated with geographical information systems (GISs) and represented by statistical and cartographic analyses and cross-tabulation matrices that indicate the dynamics of changes, loss and land gain. As the main result, we find that the areas occupied by new intensive irrigation on old rainfed farmland in the northwest Region of Murcia have increased in the last 30 years. Traditional irrigation is disappearing, and the environmental consequences (overexploitation of aquifers and decreased flows from natural sources), among others, are dire.
Genome-Wide Characterization, Evolution, and Expression Analysis of the Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Gene Family in Medicago truncatula
Authors: Meng Juan , Yang Jie , Peng Mengdi , Liu Xiaolin , He Hengbin
Published: September 2020

Leucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases (RLKs) in plants. They play roles in plant growth and developmental and physiological processes, but less is known about the functions of LRR-RLKs in Medicago truncatula. Our genome-wide analysis revealed 329 LRR-RLK genes in the M.truncatula genome. Phylogenetic and classification analysis suggested that these genes could be classified into 15 groups and 24 subgroups. A total of 321 genes were mapped onto all chromosomes, and 23 tandem duplications (TDs) involving 56 genes were distributed on each chromosome except 4. Twenty-seven M.truncatula LRR-RLK segmental duplication gene pairs were colinearly related. The exon/intron organization, motif composition and arrangements were relatively conserved among members of the same groups or subgroups. Using publicly available RNAseq data and quantitative real-time polymerase chain reaction (qRT-PCR), expression profiling suggested that LRR-RLKs were differentially expressed among different tissues, while some were expressed specifically in the roots and nodules. The expression of LRR-RLKs in A17 and 4 nodule mutants under rhizobial infection showed that 36 LRR-RKLs were highly upregulated in the sickle (skl) mutant [an ethylene (ET)-insensitive, Nod factor-hypersensitive mutant] after 12 h of rhizobium inoculation. Among these LRR-RLKs, six genes were also expressed specifically in the roots and nodules, which might be specific to the Nod factor and involved in autoregulation of the nodulation signal. Our results provide information on the LRR-RLK gene family in M. truncatula and serve as a guide for functional research of the LRR-RLKs.
Experimental Study and Calculation of a Three-Dimensional Finite Element Model of Infiltration in Drainage Asphalt Pavement
Authors: Zhang Qingsong , Ji Tianjian , Wang Zhanqi , Xiao Lei
Published: September 2020

Drainage asphalt pavement provides excellent drainage performance and driving safety, where the permeability of the pavement is the critical performance. When analyzing the permeability of drainage asphalt pavements, the previously used two-dimensional infiltration calculation model with boundary conditions deviates from the real situation. In this article, a three-dimensional infiltration finite element method was proposed to evaluate the permeability of pavement, and the feasibility of the three-dimensional infiltration finite element method to evaluate the drainage capacity of drainage asphalt pavement was verified by using the single-sided permeability test of indoor rutted slabs of drainage asphalt mixtures as an example. Finally, the effects of the longitudinal slope of the pavement on the drainage performance of the drained asphalt pavement was investigated by calculations based on the three-dimensional infiltration finite element method. The results indicated that: when the longitudinal slope was less than 6%, the influence of longitudinal slope size on the drainage capacity of drainage asphalt pavement was very small. When the gradient of the longitudinal slope from 0 to 6%, the critical drainage capacity of the pavement corresponding to each cross slope was maintained at a relatively stable value.
Early Loading of Mandibular Molar Single Implants: 1 Year Results of a Randomized Controlled Clinical Trial
Authors: Lee Jungwon , Lim Young-Jun , Kim Bongju , Koo Ki-Tae
Published: September 2020

The purpose of this study was to compare the implant survival, peri-implant marginal bone level, and peri-implant soft tissue of three different types of implants. This was performed with an early loading protocol, using a complete digital workflow, for one year of follow-up. Twenty-four patients with a single missing tooth in the mandibular posterior region were randomly assigned to the control group (SLActive Bone level implant; Institut Straumann AG, Basel, Switzerland), experiment group 1 (CMI IS-III Active implant; Neobiotech Co., Seoul, Korea), and experiment group 2 (CMI IS-III HActive implant; Neobiotech Co., Seoul, Korea). For each patient, a single implant was installed using the surgical template, and all prostheses were fabricated using a computer-aided design/computer-aided manufacturing system on a 3-dimensional model. A provisional prosthesis was implanted at 4 weeks, and a definitive monolithic zirconia prosthesis was substituted 12 weeks following the implant placement. The implant stability quotient (ISQ) and peri-implant soft tissue parameters were measured, and periapical radiographs were taken at 1, 3, 4, 8, 12, 24, 36, and 48 weeks after implant placements. Seven implants in the control group, nine implants in the experiment 1 group, and eight implants in the experiment 2 group were analyzed. There were no significant differences among the three groups in terms of insertion torque, ISQ values between surgery and 8 weeks of follow-up, marginal bone loss at 48 weeks of follow-up, and peri-implant soft tissue parameters (P > 0.05). Statistically significant differences in ISQ values were observed between the control and experiment 1 groups, and the control and experiment 2 groups at the 12 to 48 weeks’ follow-ups. Within the limits of this prospective study, an early loading protocol can be applied as a predictable treatment modality in posterior mandibular single missing restorations, achieving proper primary stability.
A Controllable Plasmonic Resonance in a SiC-Loaded Single-Polarization Single-Mode Photonic Crystal Fiber Enables Its Application as a Compact LWIR Environmental Sensor
Authors: Yang Tianyu , Ding Can , Ziolkowski Richard W. , Guo Y. Jay
Published: September 2020

Near-perfect resonant absorption is attained in a single-polarization single-mode photonic crystal fiber (SPSM PCF) within the long-wave infrared (LWIR) range from 10 to 11 μm. The basic PCF design is a triangular lattice-based cladding of circular air holes and a core region augmented with rectangular slots. A particular set of air holes surrounding the core is partially filled with SiC, which exhibits epsilon near-zero (ENZ) and epsilon negative (ENG) properties within the wavelength range of interest. By tuning the configuration to have the fields of the unwanted fundamental and all higher order modes significantly overlap with the very lossy ENG rings, while the wanted fundamental propagating mode is concentrated in the core, the SPSM outcome is realized. Moreover, a strong plasmonic resonance is attained by adjusting the radii of the resulting cylindrical core-shell structures. The cause of the resonance is carefully investigated and confirmed. The resonance wavelength is shown to finely shift, depending on the relative permittivity of any material introduced into the PCF’s air holes, e.g., by flowing a liquid or gas in them. The potential of this plasmonic-based PCF structure as a very sensitive, short length LWIR spectrometer is demonstrated with an environmental monitoring application.
The Transmittance Modulation of ZnO/Cu/ZnO Transparent Conductive Electrodes Prepared on Glass Substrates
Authors: Choi Dooho
Published: September 2020

With the explosive development of optoelectronic devices, the need for high-performance transparent conductive (TCE) electrodes for optoelectronic devices has been increasing accordingly. The two major TCE requirements are (1) visible light average transmittance higher than 80% and (2) sheet resistance lower than 10 Ω/sq. In this study, we investigated the critical role of the top and bottom ZnO thicknesses for the ZnO/Cu/ZnO electrodes prepared on glass substrates. It was shown that the required Cu thickness to meet the conductivity requirement is 8 nm, which was fixed and then the thicknesses of the top and ZnO layers were independently varied to experimentally determine the optimized conditions for optical transparency. The thicknesses of the top and bottom ZnO layers were both found to significantly affect the peak transmittance as well as the average visible light transmittance. The ZnO/Cu/ZnO electrode exhibits peak and average transmittance of 95.4% and 87.4%, excluding the transmittance of glass substrates, along with a sheet resistance of 9.7 Ω/sq, with a corresponding Haacke’s figure of merit (φH=Tave10Rs) of 0.064, which exceeds the reported value for the ZnO/Cu/ZnO electrodes, manifesting the need of experimental optimization in this study.
Study of the Active Carbon from Used Coffee Grounds as the Active Material for a High-Temperature Stable Supercapacitor with Ionic-Liquid Electrolyte
Authors: Biegun Marcin , Dymerska Anna , Chen Xuecheng , Mijowska Ewa
Published: September 2020

This study reveals a simple approach to recycle wasted coffee grounds into highly valuable carbon material with superior electrochemical performance. Activated carbon prepared from wasted coffee grounds has been formed via hydrothermal acidic hydrolysis followed by a KOH chemical activation at 800 ∘C. To understand the electrochemical properties of the sample, a set of characterization tools has been utilized: N2 and CO2 adsorption–desorption isotherms, thermal gravimetric analysis, Fourier transform infrared spectroscopy, Raman spectroscopy and scanning electron microscopy. The specific surface area obtained from a Brunner–Emmett–Teller (BET) analysis reached 2906±19m2g−1. Prepared sample (designated as ACG-800KOH) was tested as electrode material in an electric double layer capacitor (EDLC) device with ionic liquid PYR13-TFSI as an electrolyte. The EDLC test was conducted at temperatures ranging from 20 to 120 ∘C. The specific material capacitance reached 178 Fg−1 measured at 20 ∘C and 50 A g−1 and was in the range 182 to 285 Fg−1 at the 20 to 120 ∘C temperature range.
Cyclic Loading Performance of Radius-Cut Double Coke-Shaped Strip Dampers
Authors: Bae Jaehoon , Lee Chang-Hwan , Park Minjae , Alemayehu Robel Wondimu , Ryu Jaeho , Kim Youngsik , Ju Young K.
Published: September 2020

Conventional slit dampers are widely used for the purpose of seismic retrofitting, however, the structure of these dampers is susceptible to fractures, due to stress concentration at the ends of the strips in the event of large earthquakes. To address this issue, a novel radius-cut coke-shaped strip damper featuring improved ductility is proposed herein. This damper was developed based on the moment distribution over the strip when both its ends were constrained. The height-to-width ratio of the strip was increased to induce bending rather than shear deformation, and the reduced beam section method was employed. A radius-cut section was used to intentionally focus the stress to induce the plastic hinge. This reduced the fracture fragility of the specimen, resulting in an increased inelastic deformation capacity. Cyclic loading tests were conducted to verify damping performance against earthquakes. Experiments and finite element analyses proved that the coke-shaped damper exhibits improved ductility. The final fracture occurred in the radius-cut section after sufficient energy dissipation during cyclic loading. The results also indicated further improvements in strength due to the membrane effect under cyclic loading, caused by the tensile resistance of the strip due to its constrained ends.
Detection and Quantification of Cracking in Concrete Aggregate through Virtual Data Fusion of X-Ray Computed Tomography Images
Authors: Oesch Tyler , Weise Frank , Bruno Giovanni
Published: September 2020

In this work, which is part of a larger research program, a framework called “virtual data fusion” was developed to provide an automated and consistent crack detection method that allows for the cross-comparison of results from large quantities of X-ray computed tomography (CT) data. A partial implementation of this method in a custom program was developed for use in research focused on crack quantification in alkali-silica reaction (ASR)-sensitive concrete aggregates. During the CT image processing, a series of image analyses tailored for detecting specific, individual crack-like characteristics were completed. The results of these analyses were then “fused” in order to identify crack-like objects within the images with much higher accuracy than that yielded by any individual image analysis procedure. The results of this strategy demonstrated the success of the program in effectively identifying crack-like structures and quantifying characteristics, such as surface area and volume. The results demonstrated that the source of aggregate has a very significant impact on the amount of internal cracking, even when the mineralogical characteristics remain very similar. River gravels, for instance, were found to contain significantly higher levels of internal cracking than quarried stone aggregates of the same mineralogical type.
Biocomposites from Organic Solid Wastes Derived Biochars: A Review
Authors: Zhang Qingfa , Cai Hongzhen , Yi Weiming , Lei Hanwu , Liu Haolu , Wang Weihong , Ruan Roger
Published: September 2020

The replacement of natural fiber with biochars to prepare biocomposites has attracted widespread attention recently. Biochar has unique properties, including the porous structure, large specific surface area, high thermal stability, good conductivity, renewable and abundant feedstock source, and environmental friendliness, which provide excellent properties, environmental benefits, and low production costs for biochar-based composites. Biocomposites from organic solid waste-derived biochars show good prospects worldwide in terms of positive social, environmental, and economic impacts. This paper reviews current biochars, elucidates the effects of biochars on the characteristics and performance of biochar composites, and points out the challenges and future development prospects of biochar composites.
Investigation of the Mechanical Properties of a Carbon Fibre-Reinforced Nylon Filament for 3D Printing
Authors: Calignano Flaviana , Lorusso Massimo , Roppolo Ignanio , Minetola Paolo
Published: September 2020

Additive manufacturing (i.e., 3D printing) has rapidly developed in recent years. In the recent past, many researchers have highlighted the development of in-house filaments for fused filament fabrication (FFF), which can extend the corresponding field of application. Due to the limited mechanical properties and deficient functionality of printed polymer parts, there is a need to develop printable polymer composites that exhibit high performance. This study analyses the actual mechanical characteristics of parts fabricated with a low-cost printer from a carbon fibre-reinforced nylon filament. The results show that the obtained values differ considerably from the values presented in the datasheets of various filament suppliers. Moreover, the hardness and tensile strength are influenced by the building direction, the infill percentage, and the thermal stresses, whereas the resilience is affected only by the building direction. Furthermore, the relationship between the mechanical properties and the filling factor is not linear.
Numerical Simulation of Flow over Non-Linearly Stretching Sheet Considering Chemical Reaction and Magnetic Field
Authors: Razzaghi Mohsen , Baharifard Fatemeh , Parand Kourosh
Published: September 2020

The purpose of this paper is to investigate a system of differential equations related to the viscous flow over a stretching sheet. It is assumed that the intended environment for the flow includes a chemical reaction and a magnetic field. The governing equations are defined on the semi-finite domain and a numerical scheme, namely rational Gegenbauer collocation method is applied to solve it. In this method, the problem is solved in its main interval (semi-infinite domain) and there is no need to truncate it to a finite domain or change the domain of the problem. By carefully examining the effect of important physical parameters of the problem and comparing the obtained results with the answers of other methods, we show that despite the simplicity of the proposed method, it has a high degree of convergence and good accuracy.
On Designing Non-Parametric EWMA Sign Chart under Ranked Set Sampling Scheme with Application to Industrial Process
Authors: Ali Saber , Abbas Zameer , Nazir Hafiz Zafar , Riaz Muhammad , Zhang Xingfa , Li Yuan
Published: September 2020

Statistical process control (SPC) tools are used for the investigation and identification of unnatural variations in the manufacturing, industrial, and service processes. The control chart, the basic and the most famous tool of SPC, is used for process monitoring. Generally, control charts are constructed under normality assumption of the quality characteristic of interest, but in practice, it is quite hard to hold the normality assumption. In such situations, parametric charts tend to offer more frequent false alarms and invalid out-of-control performance. To rectify these problems, non-parametric control charts are used, as these have the same in-control run length properties for all the continuous distributions and are known as in-control robust. This study intends to develop a new non-parametric exponentially weighted moving average (NPEWMA) chart based on sign statistics under a ranked set sampling scheme that is hereafter named (NPREWMA-SN). The run-length profiles of the NPREWMA-SN chart are computed using the Monte Carlo simulation method. The proposed scheme is compared with NPEWMA-SN and classical EWMA-X¯ charts, using different run length measures. The comparison reveals the in-control robustness and superiority of the proposed scheme over its competitors in detecting all kinds of shifts in the process location. A practical application related to the substrate manufacturing process is included to show the demonstration of the proposed chart.
Progressive Iterative Approximation with Preconditioners
Authors: Liu Chengzhi , Liu Zhongyun
Published: September 2020

The progressive iterative approximation (PIA) plays an important role in curve and surface fitting. By using the diagonally compensated reduction of the collocation matrix, we propose the preconditioned progressive iterative approximation (PPIA) to improve the convergence rate of PIA. For most of the normalized totally positive bases, we show that the presented PPIA can accelerate the convergence rate significantly in comparison with the weighted progressive iteration approximation (WPIA) and the progressive iterative approximation with different weights (DWPIA). Furthermore, we propose an inexact variant of the PPIA (IPPIA) to reduce the computational complexity of the PPIA. We introduce the inexact solver of the preconditioning system by employing some state-of-the-art iterative methods. Numerical results show that both the PPIA and the IPPIA converge faster than the WPIA and DWPIA, while the elapsed CPU times of the PPIA and IPPIA are less than those of the WPIA and DWPIA.