bluuebunny/crossref_metadata_2025 huggingface dataset:
The Running-in of Lubricated Metal-Metal Contacts—A Review on Ultra-Low Wear Systems
Authors: Scherge Matthias
Published: June 2018

The running-in of lubricated metal–metal contacts leading to ultra-low wear is inseparably connected with the formation of the third body and vice versa. Adequate tribological stressing provides the system with a power density that leads to complex changes of topography, near-surface morphology and chemical composition. During the running-in these changes proceed until the system shows small friction and ultra-low wear rates and performs stable with low sensitivity to external perturbations. By means of high-resolution wear measurement as well as physical and chemical analysis the capability of a tribological system to develop the third body can be determined. Moreover, the running-in can be controlled by sample finishing, oil additivation and the sequence of initial stressing steps. This contribution summarizes 20 years of own research on ultra-low wear systems and its applications.
Significance of operative parameters on outcomes after transurethral resection of the prostate
Authors: Milonas Daimantas
Published: June 2018

Objective. The aim of this prospective study was to establish the influence of operative parameters on outcomes after transurethral resection of the prostate. Materials and methods. In this prospective case series study, 89 patients underwent transurethral resection of the prostate. The standardized protocol was used to investigate the impact of operative parameters (resected tissue weight, residual prostate weight, and residual prostatic weight ratio [total prostate volume – resected tissue weight / total prostate volume]) on outcomes after six months following transurethral resection of the prostate. The evaluation of treatment efficacy was done using the criteria of the Second International Consultation on Benign Prostatic Hyperplasia. All postoperative results were categorized as excellent, good, fair, or none. Treatment was considered effective when the postoperative results were excellent and good, and ineffective when results were fair and none. Results. Treatment was effective for 85.4% and ineffective for 14.6% of the patients. The univariate analysis of operative parameters detected the residual prostatic weight ratio (cutoff value, 0.71; P<0.001; sensitivity, 0.62; specificity, 0.96; OR, 39.47) as the strongest independent predictor of ineffective outcome. Logistic regression analysis revealed two important parameters of unfavorable outcomes: residual prostatic weight ratio (cutoff value, 0.71; P<0.001; OR, 62.16) and residual prostate weight (cutoff value, 26.6 mL; P=0.013; OR, 9.98). When the values of both these parameters were lower than their cutoff values, the probability of an ineffective outcome was reduced to 3%; however, when they were higher, the probability of an unfavorable outcome was increased to 95%. Conclusions. Residual prostatic weight ratio and residual prostatic weight are significant operative parameters for the prediction of outcomes after transurethral resection of the prostate.
Cisplatin increases urinary sodium excretion in rats: gender-related differences
Authors: Stakišaitis Donatas , Dudėnienė Giedrė , Jankūnas Rimas , Graželienė Gražina , Didžiapetrienė Janina , Pundzienė Birutė
Published: June 2018

Objective. There are well-documented reports of cisplatin-associated hyponatremia in the literature, but there are no data on gender-dependent differences. The aim of the present study was to define characteristics of 24-hour urinary sodium excretion in young adult Wistar rats of both genders and to evaluate the gender-related effect of cisplatin. Materials and methods. Twelve control Wistar rats (6 males and 6 females) and 12 cisplatintreated Wistar rats (6 males and 6 females) after a single and repeated injection of cisplatin (once a day for 3 days) at a dose of 2.5 mg/kg body weight into the caudal vein were examined. The experiment was carried out by measuring 24-h urinary sodium, potassium, chloride, magnesium, creatinine excretion and pH in the urine of age-matched male and female rats. Results. The 24-h urinary sodium excretion, sodium/chloride ratio, and diuresis showed no gender-related differences in control rats. After a single administration of 2.5 mg/kg cisplatin, 24-h urinary sodium excretion was not significantly higher in cisplatin-treated rats than in gender-matched controls. After repeated cisplatin administration, 24-h urinary sodium excretion was significantly higher in cisplatin-treated male rats as compared to matched controls (P<0.05). No such effect was found in cisplatin-treated female rats. Conclusion. The study data show that cisplatin enhances urinary sodium excretion in male but not in female rats. The mechanism of such a gender-related effect is not yet clear. Further investigations are necessary to elucidate the mechanism of this pharmacological effect of cisplatin.
The role of high-grade prostatic intraepithelial neoplasia for biochemical relapse of prostate carcinoma after radical prostatectomy
Authors: Auškalnis Stasys , Milonas Daimantas , Jievaltas Mindaugas , Vaičiūnas Kęstutis , Mickevičius Antanas , Gudinavičienė Inga
Published: June 2018

The objective of the study was to evaluate the relationship between high-grade intraepithelial neoplasia diagnosed after radical retropubic prostatectomy and the clinical and pathological characteristics of prostate cancer, and to evaluate the time to biochemical relapse of the disease within the groups of high-grade prostatic intraepithelial neoplasia (HGPIN) and non-HGPIN patients. Material and methods. Patients, clinically diagnosed with local prostate carcinoma at the Clinic of Urology, Kaunas University of Medicine, during 2003–2007 and treated with radical retropubic prostatectomies, were distributed into two groups according to the HGPIN detected in the postoperative material: HGPIN and non-HGPIN. The two groups were compared in terms of preoperative and postoperative characteristics. The patients who were followed up for at least 12 months were included into the study. The biochemical relapse of prostate cancer was determined if there were two consecutive rises of prostate-specific antigen (PSA) level above 0.2 ng/mL or according to the attending physician’s opinion, there was a need for adjuvant treatment even with onetime rise of PSA level above 0.2 ng/mL. Results. There was no significant difference between the HGPIN and non-HGPIN groups in terms of time to biochemical relapse and frequency of biochemical relapses, time before surgery, the timing of the HGPIN diagnosis, age, or PSA level. After radical prostatectomy, patients in the HGPIN group were found to have significantly more often poorer cancer cell differentiation according to the Gleason score (≥7 vs. <7; P=0.001) and higher TNM stage (T3a,b vs. T2a,b,c; P=0.001). Fewer positive resection margins were diagnosed in the HGPIN group (P=0.05). The groups did not differ in terms of the degree of differentiation according to the Gleason score or perineural invasion (P=0.811 and P=0.282, respectively). Conclusions. HGPIN was more often associated with the characteristics of the poor prognosis for relapse of prostate cancer: poorer tumor cell differentiation according to the Gleason score and more cases of higher TNM stage. HGPIN did not have any influence on biochemical relapse of the disease during the short-term follow-up.
Accreditation Standard Guideline Initiative for Tai Chi and Qigong Instructors and Training Institutions
Authors: Oh Byeongsang , Yeung Albert , Klein Penelope , Larkey Linda , Ee Carolyn , Zaslawski Chris , Knobf Tish , Payne Peter , Stener-Victorin Elisabet , Lee Richard , Choi Whanseok , Chun Mison , Bonucci Massimo , Lang Hanne-Doris , Pavlakis Nick , Boyle Fran , Clarke Stephen , Back Michael , Yang Peiying , Wei Yulong , Guo Xinfeng , Weng Chi-hsiu D. , Irwin Michael R. , Elfiky Aymen A. , Rosenthal David
Published: June 2018

Evidence of the health and wellbeing benefits of Tai Chi and Qigong (TQ) have emerged in the past two decades, but TQ is underutilized in modern health care in Western countries due to lack of promotion and the availability of professionally qualified TQ instructors. To date, there are no government regulations for TQ instructors or for training institutions in China and Western countries, even though TQ is considered to be a part of Traditional Chinese medicine that has the potential to manage many chronic diseases. Based on an integrative health care approach, the accreditation standard guideline initiative for TQ instructors and training institutions was developed in collaboration with health professionals, integrative medicine academics, Tai Chi and Qigong master instructors and consumers including public safety officers from several countries, such as Australia, Canada, China, Germany, Italy, Korea, Sweden and USA. In this paper, the rationale for organizing the Medical Tai Chi and Qigong Association (MTQA) is discussed and the accreditation standard guideline for TQ instructors and training institutions developed by the committee members of MTQA is presented. The MTQA acknowledges that the proposed guidelines are broad, so that the diversity of TQ instructors and training institutions can be integrated with recognition that these guidelines can be developed with further refinement. Additionally, these guidelines face challenges in understanding the complexity of TQ associated with different principles, philosophies and schools of thought. Nonetheless, these guidelines represent a necessary first step as primary resource to serve and guide health care professionals and consumers, as well as the TQ community.
Shall We Focus on the Eosinophil to Guide Treatment with Systemic Corticosteroids during Acute Exacerbations of Chronic Obstructive Pulmonary Disease (COPD)? CON
Authors: Marcos Pedro J , López-Campos José Luis
Published: June 2018

The employment of systemic corticosteroids in the treatment of acute exacerbations of chronic obstructive pulmonary disease (COPD) has been shown to improve airway limitation, decrease treatment failure and risk of relapse, and may improve symptoms in addition to decreasing the length of hospital stay. Nowadays, all clinical guidelines recommend systemic corticosteroids to treat moderate or severe COPD exacerbations. However, their use is associated with potential side effects, mainly hyperglycemia. In the era of precision medicine, the possibility of employing blood eosinophil count has emerged as a potential way of optimizing therapy. Issues regarding the intra-individual variability of blood eosinophil count determination, a lack of clear data regarding the real prevalence of eosinophilic acute exacerbations, the fact that previously published studies have demonstrated the benefit of systemic corticosteroids irrespective of eosinophil levels, and especially the fact that there is only one well-designed study justifying this approach have led us to think that we are not ready to use eosinophil count to guide treatment with systemic corticosteroids during acute exacerbations of COPD.
Miniaturized Optical Resolution Photoacoustic Microscope Based on a Microelectromechanical Systems Scanning Mirror
Authors: Qi Weizhi , Chen Qian , Guo Heng , Xie Huikai , Xi Lei
Published: June 2018

In this paper, we report a miniaturized optical resolution photoacoustic microscopy system based on a microelectromechanical system (MEMS) scanning mirror. A two-dimensional MEMS scanning mirror was used to achieve raster scanning of the excitation optical focus. The wideband photoacoustic signals were detected by a flat ultrasound transducer with a center frequency of 10 MHz and an active area of 2 mm in diameter. The size and weight of this device were 60 mm × 30 mm × 20 mm and 40 g, respectively. We evaluated this system using sharp blades, carbon fibers, and a silver strip target. In vivo experiments of imaging vasculatures in the mouse ear, brain, and human lip were completed to demonstrate its potential for biological and clinical applications.
Chopper-Stabilized Instrumentation Amplifier with Automatic Frequency Tuning Loop
Authors: Wu Chen-Mao , Chen Hsiao-Chin , Yen Ming-Yu , Yang San-Ching
Published: June 2018

A variable-gain chopper-stabilized instrumentation amplifier (chopper IA), which employs a low pass filter (LPF) to attenuate the up-converted noise at the chopping frequency, is presented. The circuit is designed and fabricated with Taiwan Semiconductor Manufacturing Company (TSMC) (Hsinchu, Taiwan) 0.18 μm complementary metal-oxide-semiconductor (CMOS) technology. Consuming 1.1 mW from a 1.2 V supply voltage, the chopper IA achieves a variable gain of 20.7–48.5 dB, with a minimum bandwidth of 6.7 kHz and a common-mode rejection ratio (CMRR) of 95 dB below 10 kHz. By using the chopper technique, the input-referred noise of the chopper IA can be reduced to 0.28 μVrms (0~96 kHz), with a chopping frequency of 83.3 kHz. An automatic frequency tuning loop (ATL) is employed to adjust the corner frequency of the LPF dynamically so that the frequency ratio between the chopping frequency and the LPF corner frequency is 8.3, ensuring a noise reduction of 36.7 dB.
Particle-Crushing Characteristics and Acoustic-Emission Patterns of Crushing Gangue Backfilling Material under Cyclic Loading
Authors: Li Junmeng , Huang Yanli , Chen Zhongwei , Li Meng , Qiao Ming , Kizil Mehmet
Published: June 2018

In solid backfilling coal mining (SBCM), the crushed gangue backfilling material (CGBM) is generally compacted circularly by a compaction machine in order to reduce its compressibility. In this cyclic compaction process, the particles are crushed, which has a significant effect on the deformation resistance of CGBM. However, the deformation resistance of CGBM is critical for controlling overburden strata movement and ground surface subsidence. This study implemented an experimental approach to investigate the particle-crushing characteristics and acoustic-emission (AE) characteristics of CGBM during constant-amplitude cyclic loading (CACL). At the same time, the relationship between particle crushing and AE signals was established. The results showed that the gangue particles were generally in the shape of irregular convex polyhedrons with more edges and angles that were prone to breakage. It also demonstrated that both the crushing ratio (Bg) and the newly produced fine granule content increased with the cyclic loading times. The content of newly generated fine particles can reflect the particle-crushing conditions to a certain extent. What is more, it was found that the CGBM samples exhibited an apparent Felicity effect during CACL, and AE signals were the most active during the first loading cycle. The crushing ratio of CGBM was highly correlated to the AE signals, which indicated that AE signals can be used to reflect the particle-crushing situation of CGBM. This study is of great significance for obtaining an in-depth understanding of the mechanical properties of CGBM, as well as providing guidance for the engineering practice of SBCM.
Chiral 2-Aminobenzimidazole as Bifunctional Catalyst in the Asymmetric Electrophilic Amination of Unprotected 3-Substituted Oxindoles
Authors: Benavent Llorenç , Baeza Alejandro , Freckleton Megan
Published: June 2018

The use of readily available chiral trans-cyclohexanediamine-benzimidazole derivatives as bifunctional organocatalysts in the asymmetric electrophilic amination of unprotected 3-substituted oxindoles is presented. Different organocatalysts were evaluated; the most successful one contained a dimethylamino moiety (5). With this catalyst under optimized conditions, different oxindoles containing a wide variety of substituents at the 3-position were aminated in good yields and with good to excellent enantioselectivities using di-tert-butylazodicarboxylate as the aminating agent. The procedure proved to be also efficient for the amination of 3-substituted benzofuranones, although with moderate results. A bifunctional role of the catalyst, acting as Brønsted base and hydrogen bond donor, is proposed according to the experimental results observed.
[18F]FEPPA a TSPO Radioligand: Optimized Radiosynthesis and Evaluation as a PET Radiotracer for Brain Inflammation in a Peripheral LPS-Injected Mouse Model
Authors: Vignal Nicolas , Cisternino Salvatore , Rizzo-Padoin Nathalie , San Carine , Hontonnou Fortune , Gelé Thibaut , Declèves Xavier , Sarda-Mantel Laure , Hosten Benoît
Published: June 2018

[18F]FEPPA is a specific ligand for the translocator protein of 18 kDa (TSPO) used as a positron emission tomography (PET) biomarker for glial activation and neuroinflammation. [18F]FEPPA radiosynthesis was optimized to assess in a mouse model the cerebral inflammation induced by an intraperitoneal injection of Salmonella enterica serovar Typhimurium lipopolysaccharides (LPS; 5 mg/kg) 24 h before PET imaging. [18F]FEPPA was synthesized by nucleophilic substitution (90 °C, 10 min) with tosylated precursor, followed by improved semi-preparative HPLC purification (retention time 14 min). [18F]FEPPA radiosynthesis were carried out in 55 min (from EOB). The non-decay corrected radiochemical yield were 34 ± 2% (n = 17), and the radiochemical purity greater than 99%, with a molar activity of 198 ± 125 GBq/µmol at the end of synthesis. Western blot analysis demonstrated a 2.2-fold increase in TSPO brain expression in the LPS treated mice compared to controls. This was consistent with the significant increase of [18F]FEPPA brain total volume of distribution (VT) estimated with pharmacokinetic modelling. In conclusion, [18F]FEPPA radiosynthesis was implemented with high yields. The new purification/formulation with only class 3 solvents is more suitable for in vivo studies.
Comparison between Multi-Linear- and Radial-Basis-Function-Neural-Network-Based QSPR Models for The Prediction of The Critical Temperature, Critical Pressure and Acentric Factor of Organic Compounds
Authors: Banchero Mauro , Manna Luigi
Published: June 2018

Critical properties and acentric factor are widely used in phase equilibrium calculations but are difficult to evaluate with high accuracy for many organic compounds. Quantitative Structure-Property Relationship (QSPR) models are a powerful tool to establish accurate correlation between molecular properties and chemical structure. QSPR multi-linear (MLR) and radial basis-function-neural-network (RBFNN) models have been developed to predict the critical temperature, critical pressure and acentric factor of a database of 306 organic compounds. RBFNN models provided better data correlation and higher predictive capability (an AAD% of 0.92–2.0% for training and 1.7–4.8% for validation sets) than MLR models (an AAD% of 3.2–8.7% for training and 6.2–12.2% for validation sets). The RMSE of the RBFNN models was 20–30% of the MLR ones. The correlation and predictive performances of the models for critical temperature were higher than those for critical pressure and acentric factor, which was the most difficult property to predict. However, the RBFNN model for the acentric factor resulted in the lowest RMSE with respect to previous literature. The close relationship between the three properties resulted from the selected molecular descriptors, which are mostly related to molecular electronic charge distribution or polar interactions between molecules. QSPR correlations were compared with the most frequently used group-contribution methods over the same database of compounds: although the MLR models provided comparable results, the RBFNN ones resulted in significantly higher performance.
Pre-treatment of Oil Palm Biomass for Fermentable Sugars Production
Authors: Rizal Nur , Ibrahim Mohamad , Zakaria Mohd , Abd-Aziz Suraini , Yee Phang , Hassan Mohd
Published: June 2018

Malaysia is the second largest palm oil producer in the world and this industry generates more than 80 million tonnes of biomass every year. When considering the potential of this biomass to be used as a fermentation feedstock, many studies have been conducted to develop a complete process for sugar production. One of the essential processes is the pre-treatment to modify the lignocellulosic components by altering the structural arrangement and/or removing lignin component to expose the internal structure of cellulose and hemicellulose for cellulases to digest it into sugars. Each of the pre-treatment processes that were developed has their own advantages and disadvantages, which are reviewed in this study.
Piplartine Analogues and Cytotoxic Evaluation against Glioblastoma
Authors: Da Nóbrega Flávio Rogério , Ozdemir Ozlem , Nascimento Sousa Sheila Cristina S. , Barboza Joice Nascimento , Turkez Hasan , De Sousa Damião Pergentino
Published: June 2018

Piplartine (1) is an alkamide extracted from plants of the genus Piper which shows several pharmacological properties, including antitumor activity. To improve this activity, a series of analogues based on 1 have been synthesized by esterification and amidation using the 3,4,5-trimethoxycinnamic acid-like starting material. During the study, the moieties 3-(3,4,5-trimethoxyphenyl)acrylate and 3-(3,4,5-trimethoxyphenyl)acrylamide were maintained on esters and amides respectively. Meanwhile, functional changes were exploited, and it was revealed that the presence of two aromatic rings in the side-chain was important to improve the cytotoxic activity against the U87MG cell line, such as the compound (E)-benzhydryl 3-(3,4,5-trimethoxyphenyl)acrylate (10), an ester that exhibited strong cytotoxicity and a similar level of potency to that of paclitaxel, a positive control. Compound 10 had a marked concentration-dependent inhibitory effect on the viability of the U87MG cell line with apoptotic and oxidative processes, showing good potential for altering main molecular pathways to prevent tumor development. Moreover, it has strong bioavailability with non-genotoxic and non-cytotoxic properties on human blood cells. In conclusion, the findings of the present study demonstrated that compound 10 is a promising agent that may find applications combatting diseases associated with oxidative stress and as a prototype for the development of novel drugs used in the treatment of glioblastoma.
Four New Sesquiterpenoids from the Roots of Diarthron Tianschanica with Their Antineoplastic Activity
Authors: Sun Dong-Xue , Zhao Dan , Wei Hong-Yan , Ma Xiao-Ling , Shi Lei-Ling , Zhang Jing
Published: June 2018

Four new sesquiterpenoids, known as diarthronchas A–D (1–4), and one known daphnauranol B (5) were isolated from the methanol extract of the roots of Diarthron tianschanica. The compounds structures were determined on the basis of spectroscopic data. All of the isolated compounds were profiled for their antineoplastic activity.
Cobalt Complex with Thiazole-Based Ligand as New Pseudomonas aeruginosa Quorum Quencher, Biofilm Inhibitor and Virulence Attenuator
Authors: Borges Anabela , Simões Manuel , Todorović Tamara R. , Filipović Nenad R. , García-Sosa Alfonso T.
Published: June 2018

Pseudomonas aeruginosa is one of the most dreaded human pathogens, because of its intrinsic resistance to a number of commonly used antibiotics and ability to form sessile communities (biofilms). Innovative treatment strategies are required and that can rely on the attenuation of the pathogenicity and virulence traits. The interruption of the mechanisms of intercellular communication in bacteria (quorum sensing) is one of such promising strategies. A cobalt coordination compound (Co(HL)2) synthesized from (E)-2-(2-(pyridin-2-ylmethylene)hydrazinyl)-4-(p-tolyl)thiazole (HL) is reported herein for the first time to inhibit P. aeruginosa 3-oxo-C12-HSL-dependent QS system (LasI/LasR system) and underling phenotypes (biofilm formation and virulence factors). Its interactions with a possible target, the transcriptional activator protein complex LasR-3-oxo-C12-HSL, was studied by molecular modeling with the coordination compound ligand having stronger predicted interactions than those of co-crystallized ligand 3-oxo-C12-HSL, as well as known-binder furvina. Transition metal group 9 coordination compounds may be explored in antipathogenic/antibacterial drug design.
Synthesis of 2-Mercapto-(2-Oxoindolin-3-Ylidene)Acetonitriles from 3-(4-Chloro-5H-1,2,3-Dithiazol-5-Ylidene)Indolin-2-ones
Authors: Letribot Boris , Delatouche Régis , Rouillard Hervé , Bonnet Antoine , Chérouvrier Jean-René , Domon Lisianne , Besson Thierry , Thiéry Valérie
Published: June 2018

Alkylidene oxindoles are important functional moieties and building blocks in pharmaceutical and synthetic chemistry. Our interest in biologically active compounds focused our studies on the synthesis of novel oxindoles, bearing on the exocyclic double bond at the C8, CN, and S groups. Extending the potential applications of Appel’s salt, we developed a new synthetic approach by investigating the reactions of C5-substituted 2-oxindoles with 4,5-dichloro-1,2,3-dithiazolium chloride (Appel’s salt) to give original (Z)-3-(4-chloro-5H-1,2,3-dithiazol-5-ylidene)indolin-2-one derivatives, and new 2-mercapto-(2-oxoindolin-3-ylidene)acetonitriles via a dithiazole ring-opening reaction. The work described in this article represents further applications of Appel’s salt in the conception of novel heterocyclic rings, in an effort to access original bioactive compounds. Fifteen new compounds were prepared and fully characterized.
Isolation, Structural Elucidation, and α-Glucosidase Inhibitory Activities of Triterpenoid Lactones and Their Relevant Biogenetic Constituents from Ganoderma resinaceum
Authors: Chen Xian-Qiang , Lin Li-Gen , Zhao Jing , Chen Ling-Xiao , Tang Yu-Ping , Luo De-Lun , Li Shao-Ping
Published: June 2018

Ganoderma resinaceum has been used as an ethnomedicine for lowering blood sugar. To clarify the bioactive chemical constituents contributing to lower blood sugar, chemical investigation on the fruiting bodies of Ganoderma resinaceum was conducted by chromatographic techniques, and led to the isolation of 14 compounds. Their structures were elucidated as triterpenoid lactones (1–4 and 8) and ganoderma acids (5–7 and 9–14) based on the analysis of extensive spectroscopy (mass spectrometry (MS), nuclear magnetic resonance (NMR), infrared (IR), and ultraviolet (UV)) and comparison with literature data. Compounds 3, 5, 6, and 9–14 were evaluated for α-glucosidase inhibitory activity. Compounds 1–7 are new compounds. Compounds 1–4 and 8 were characteristic of an oxaspirolactone moiety, consisting of a five-membered ether ring, a five-membered lactone ring, and a characteristic C-23 spiro carbon. It is rare for natural products that such an oxaspirolactone moiety occurred in the lanostane-type triterpenoids. Compounds 5–7 and 9–14 may be important intermediates of the biosynthetic pathways of 1–4 and 8. Compounds 1 and 2 showed more potent inhibitory activity against α-glucosidase compared with the positive control drug acarbose with IC50 value of 0.75 ± 0.018 mM and 1.64 ± 0.022 mM, respectively.
Protective Effects on 60Co-γ Radiation Damage of Pine Cone Polyphenols from Pinus koraiensis-Loaded Chitosan Microspheres In Vivo
Authors: Shao Sujuan , Yi Juanjuan , Regenstein Joe M. , Cheng Cuilin , Zhang Hua , Zhao Haitian , Wang Zhenyu
Published: June 2018

A novel chitosan microsphere for encapsulating pine cone polyphenols (PP) from P. koraiensis was successfully prepared using an emulsion crosslinking technique. The characteristics of pine polyphenol-loaded microspheres (PPM) were determined using scanning electron microscopy (SEM) and a laser particle size detector. It was found that PPMs were spherical in shape with uniform particle size distribution patterns. The drug content and encapsulation rate of the microspheres were 7.47% and 73.6%, respectively, at a Ch/GA mass ratio of 0.7. The animal experiments showed that PPM had a stronger radiation protective effect than PP. PPM significantly increased the immune organ indices, the quantity of marrow DNA, the superoxide dismutase (SOD) activity, the splenocyte proliferation index, and the phagocytosis activity of monocytes. PPM also decreased the numbers of micronuclei in bone marrow cells and malondialdehyde (MDA) levels in plasma in mice exposed to 60Co γ-irradiation. In addition, gender differences in biological responses to exposure to radiation were observed.
Molecular Identification and Taxonomic Implication of Herbal Species in Genus Corydalis (Papaveraceae)
Authors: Jiang Lu , Li Meihui , Zhao Fengxi , Chu Shanshan , Zha Liangping , Xu Tao , Peng Huasheng , Zhang Wei
Published: June 2018

Many species of Corydalis (Papaveraceae) have been used as medicinal plants in East Asia, and the most well-known species are Corydalis yanhusuo and C. decumbens in the Pharmacopoeia of China. However, authentication of these species remains problematic because of their high morphological variation. Here, we selected 14 closely related species and five genomic regions (chloroplast: matK, trnG, rbcL, psbA-trnH; nuclear: ITS) to explore the utility of DNA barcoding for authenticating these herbs. In addition, the Poisson tree process (PTP) and automatic barcode gap discovery (ABGD) were also used and compared with DNA barcoding. Our results showed that the ITS region was not suitable for molecular analysis because of its heterogeneous nature in Corydalis. In contrast, matK was an ideal region for species identification because all species could be resolved when matK was used along with the other three chloroplast regions. We found that at least five traditional identified species were lumped into one genetic species by ABGD and PTP methods; thus, highlighting the overestimation of species diversity using the morphological approach. In conclusion, our first attempt of molecular analysis of Corydalis herbs presented here successfully resolved the identification of medicinal species and encouraged their taxonomic re-assessment.
Anti-Tumorigenic Activity of Chrysin from Oroxylum indicum via Non-Genotoxic p53 Activation through the ATM-Chk2 Pathway
Authors: Nagasaka Mai , Hashimoto Ryoko , Inoue Yasumichi , Ishiuchi Kan’ichiro , Matsuno Michiyo , Itoh Yuka , Tokugawa Muneshige , Ohoka Nobumichi , Morishita Daisuke , Mizukami Hajime , Makino Toshiaki , Hayashi Hidetoshi
Published: June 2018

The p53 tumor suppressor plays critical roles in cell cycle regulation and apoptotic cell death in response to various cellular stresses, thereby preventing cancer development. Therefore, the activation of p53 through small molecules is an attractive therapeutic strategy for the treatment of cancers retaining wild-type p53. We used a library of 700 Myanmar wild plant extracts to identify small molecules that induce p53 transcriptional activity. A cell-based screening method with a p53-responsive luciferase-reporter assay system revealed that an ethanol extract of Oroxylum indicum bark increased p53 transcriptional activity. Chrysin was isolated and identified as the active ingredient in the O. indicum bark extract. A treatment with chrysin increased p53 protein expression and the p53-mediated expression of downstream target genes, and decreased cell viability in MCF7 cells, but not in p53-knockdown MCF7 cells. We also found that chrysin activated the ATM-Chk2 pathway in the absence of DNA damage. Hence, the inactivation of the ATM-Chk2 pathway suppressed p53 activation induced by chrysin. These results suggest the potential of chrysin as an anti-cancer drug through the activation of p53 without DNA damage.
The Design and Synthesis of N-Xanthone Benzenesulfonamides as Novel Phosphoglycerate Mutase 1 (PGAM1) Inhibitors
Authors: Wang Penghui , Jiang Lulu , Cao Yang , Ye Deyong , Zhou Lu
Published: June 2018

Upregulation of phosphoglycerate mutase 1 (PGAM1) has been identified as one common phenomenon in a variety of cancers. Inhibition of PGAM1 provides a new promising therapeutic strategy for cancer treatment. Herein, based on our previous work, a series of new N-xanthone benzenesulfonamides were discovered as novel PGAM1 inhibitors. The representative molecule 15h, with an IC50 of 2.1 μM, showed an enhanced PGAM1 inhibitory activity and higher enzyme inhibitory specificity compared to PGMI-004A, as well as a slightly improved antiproliferative activity.
Design, Synthesis, Anticancer Evaluation and Docking Studies of Novel Heterocyclic Derivatives Obtained via Reactions Involving Curcumin
Authors: Borik Rita M. , Fawzy Nagwa M. , Abu-Bakr Sherifa M. , Aly Magdy S.
Published: June 2018

Curcumin, a widely utilized flavor and coloring agent in food, has been shown to demonstrate powerful antioxidant, antitumor promoting and anti-inflammatory properties in vitro and in vivo. In the present work, synthesis of new heterocyclic derivatives based on Curcumin was studied. Compound 3 was synthesized via the reaction of furochromone carbaldehyde (1) with Curcumin (2) using pipredine as catalyst. Also, novel, 4,9-dimethoxy-5H-furo [3, 2-g] chromen-5-one derivatives 4a–d, 6a–d, 7, 8a–d, 9 and 10 were synthesized by the reactions of furochromone carbaldehyde (1) with different reagents (namely: appropriate amine 3a–d, appropriate hydrazine 5a–d, hydroxylamine hydrochloride, urea/thiourea, malononitrile, malononitrile with hydrazine hydrate). The structure of the synthesized products had been confirmed from their spectroscopic data (IR, 1H-NMR, 13C-NMR and mass spectra). In the present investigation, the newly synthesized products were screened using the MTT colorimetric assay for their in vitro inhibition capacity in two human cancer cell lines (hepatocellular carcinoma (HEPG2) and breast cancer (MCF-7) as well as the normal cell line (human normal melanocyte, HFB4) in comparison to the known anticancer drugs: 5-flurouracil and doxorubicin. The anticancer activity results indicated that the synthesized products 4c and 8b showed growth inhibition activity against HEPG2 cell line and synthesized products 4b and 8a showed growth inhibition activity against MCF-7, but with varying intensities in comparison to the known anticancer drugs, 5-flurouracil and doxorubicin. Cyclin dependent kinase 2 (CDK2), a major cell cycle protein, was identified as a potential molecular target of Curcumin. Furthermore, Curcumin induced G1 cell cycle arrest, which is regulated by CDK2 in cancer cells. Therefore, we used molecular modelling to study in silico the possible inhibitory effect of CDK2 by Curcumin derivatives as a possible mechanism of these compounds as anticancer agents. The molecular docking study revealed that compounds 4b, 8a and 8b were the most effective compounds in inhibiting CDk2, and, this result was in agreement with cytotoxicity assay.
Three-Dimensional Honeycomb-Like Porous Carbon with Both Interconnected Hierarchical Porosity and Nitrogen Self-Doping from Cotton Seed Husk for Supercapacitor Electrode
Authors: Chen Hui , Wang Gang , Chen Long , Dai Bin , Yu Feng
Published: June 2018

Hierarchical porous structures with surface nitrogen-doped porous carbon are current research topics of interest for high performance supercapacitor electrode materials. Herein, a three-dimensional (3D) honeycomb-like porous carbon with interconnected hierarchical porosity and nitrogen self-doping was synthesized by simple and cost-efficient one-step KOH activation from waste cottonseed husk (a-CSHs). The obtained a-CSHs possessed hierarchical micro-, meso-, and macro-pores, a high specific surface area of 1694.1 m2/g, 3D architecture, and abundant self N-doping. Owing to these distinct features, a-CSHs delivered high specific capacitances of 238 F/g and 200 F/g at current densities of 0.5 A/g and 20 A/g, respectively, in a 6 mol/L KOH electrolyte, demonstrating good capacitance retention of 84%. The assembled a-CSHs-based symmetric supercapacitor also displayed high specific capacitance of 52 F/g at 0.5 A/g, with an energy density of 10.4 Wh/Kg at 300 W/Kg, and 91% capacitance retention after 5000 cycles at 10 A/g.
Comparison between Dual-Energy X-ray Absorptiometry and Bioelectrical Impedance Analyses for Accuracy in Measuring Whole Body Muscle Mass and Appendicular Skeletal Muscle Mass
Authors: Lee Seo Young , Ahn Soyeon , Kim Young Ji , Ji Myoung Jin , Kim Kyoung Min , Choi Sung Hee , Jang Hak Chul , Lim Soo
Published: June 2018

We evaluate the accuracy of whole body muscle mass (WBMM) and appendicular skeletal muscle mass (ASMM) assessed by bioelectrical impedance analysis (BIA) using an InBody770 machine (InBody, Seoul, Korea) referenced to dual-energy X-ray absorptiometry (DXA) in 507 people (mean age 63.7 ± 10.8 years, body mass index (BMI) 25.2 ± 3.5 kg/m2). Mean WBMMs measured by BIA and DXA were 49.3 ± 6.6 kg and 46.8 ± 6.5 kg in men and 36.1 ± 4.7 kg and 34.0 ± 4.8 kg in women, respectively. The respective effect sizes and 95% confidence intervals (CIs) for the difference were 2.49 (2.22–2.76) for men, and 2.12 (1.91–2.33) for women. Mean ASMMs measured by BIA and DXA were 22.1 ± 3.3 kg and 19.9 ± 3.2 kg in men, and 15.3 ± 2.5 kg and 13.5 ± 2.2 kg in women, respectively. The respective effect sizes and 95% CIs for the difference were 2.26 (2.10–2.41) for men and 1.75 (1.65–1.87) for women. The BIA clearly overestimated WBMM by 2.28 kg and ASMM by 1.97 kg compared with DXA. Using BMI, gender, and fat percentage, we derive equations that improved the residuals to <2 kg between methods from 38.29% to 85.91% for WBMM and 52.78% to 97.02% for ASMM.
Possible Mechanism of Glucose Uptake Enhanced by Cold Atmospheric Plasma: Atomic Scale Simulations
Authors: Razzokov Jamoliddin , Yusupov Maksudbek , Bogaerts Annemie
Published: June 2018

Cold atmospheric plasma (CAP) has shown its potential in biomedical applications, such as wound healing, cancer treatment and bacterial disinfection. Recent experiments have provided evidence that CAP can also enhance the intracellular uptake of glucose molecules which is important in diabetes therapy. In this respect, it is essential to understand the underlying mechanisms of intracellular glucose uptake induced by CAP, which is still unclear. Hence, in this study we try to elucidate the possible mechanism of glucose uptake by cells by performing computer simulations. Specifically, we study the transport of glucose molecules through native and oxidized membranes. Our simulation results show that the free energy barrier for the permeation of glucose molecules across the membrane decreases upon increasing the degree of oxidized lipids in the membrane. This indicates that the glucose permeation rate into cells increases when the CAP oxidation level in the cell membrane is increased.
Enhancement of Rhodamine B Degradation by Ag Nanoclusters-Loaded g-C3N4 Nanosheets
Authors: Le Thi Mai Oanh , Lam Thi Hang , Pham Thi Nhung , Ngo Tuan Cuong , Lai Ngoc Diep , Do Danh Bich , Nguyen Van Minh
Published: June 2018

In this paper, silver (Ag) nanoclusters-loaded graphitic carbon nitride (g-C3N4) nanosheets are synthesized and their physical properties as well as photocatalytic activities are systematically investigated by different techniques. The existence of Ag atoms in the form of nanoclusters (NCs) rather than well-crystallized nanoparticles are evidenced by X-ray diffraction patterns, SEM images, and XPS spectra. The deposition of Ag nanoclusters on the surface of g-C3N4 nanosheets affect the crystal structure and slightly reduce the band gap energy of g-C3N4. The sharp decrease of photoluminescence intensity indicates that g-C3N4/Ag heterojunctions successfully prevent the recombination of photo-generated electrons and holes. The photocatalytic activities of as-synthesized photocatalysts are demonstrated through the degradation of rhodamine B (RhB) solutions under Xenon lamp irradiation. It is demonstrated that the photocatalytic activity depends strongly on the molar concentration of Ag+ in the starting solution. The g-C3N4/Ag heterojunctions prepared from 0.01 M of Ag+ starting solution exhibit the highest photocatalytic efficiency and allow 100% degradation of RhB after being exposed for 60 min under a Xenon lamp irradiation, which is four times faster than that of pure g-C3N4 nanosheets.
Effects of Major Components of Synovial Fluid on the Morphology and Wear Rate of Polyetheretherketone (PEEK) Particles under an Accelerated Wear Process
Authors: Su Chen-Ying , Huang Shih-Shuan , Fang Hsu-Wei
Published: June 2018

Wear particle-induced biological responses are the major factors for the failure of total joint arthroplasties, but it is possible to improve the lubrication and reduce the wear of an artificial joint system. Polyetheretherketone (PEEK), with ultra-high molecular weight polyethylene, is a suitable bearing material due to its resistance to fatigue strain. However, the effects of major compositions of synovial fluid on the wear of PEEK are unclear. We characterized the effects of three major components of synovial fluid including albumin, globulin, and phospholipids on the morphology and wear rate of PEEK wear particles. Our results demonstrated that the concentrations of albumin and globulin could affect the morphology of PEEK wear particles. In addition, a higher concentration of globulin and phospholipids (12.5 mg/mL) resulted in an increase in the amount of wear particles by 2.8- and 1.7-fold, respectively. In contrast, increasing albumin caused a reduction of wear particle numbers. These results indicate increasing concentration of albumin or reducing concentration of globulin or phospholipids has a better effect on reducing the numbers of wear particles and provides a potential solution of reducing PEEK wear particles, thus it can be more effectively applied in other biomedical systems.
High Temporal Resolution Refractivity Retrieval from Radar Phase Measurements
Authors: López Rubén Nocelo , Río Verónica Santalla del
Published: June 2018

Knowledge of the spatial and temporal variability of near-surface water vapor is of great importance to successfully model reliable radio communications systems and forecast atmospheric phenomena such as convective initiation and boundary layer processes. However, most current methods to measure atmospheric moisture variations hardly provide the temporal and spatial resolutions required for detection of such atmospheric processes. Recently, considering the high correlation between refractivity variations and water vapor pressure variations at warm temperatures, and the good temporal and spatial resolution that weather radars provide, the measurement of the refractivity with radar became of interest. Firstly, it was proposed to estimate refractivity variations from radar phase measurements of ground-based stationary targets returns. For that, it was considered that the backscattering from ground targets is stationary and the vertical gradient of the refractivity could be neglected. Initial experiments showed good results over flat terrain when the radar and target heights are similar. However, the need to consider the non-zero vertical gradient of the refractivity over hilly terrain is clear. Up to date, the methods proposed consider previous estimation of the refractivity gradient in order to correct the measured phases before the refractivity estimation. In this paper, joint estimation of the refractivity variations at the radar height and the refractivity vertical gradient variations using scan-to-scan phase measurement variations is proposed. To reduce the noisiness of the estimates, a least squares method is used. Importantly, to apply this algorithm, it is not necessary to modify the radar scanning mode. For the purpose of this study, radar data obtained during the Refractivity Experiment for H 2 O Research and Collaborative Operational Technology Transfer (REFRACTT_2006), held in northeastern Colorado (USA), are used. The refractivity estimates obtained show a good performance of the algorithm proposed compared to the refractivity derived from two automatic weather stations located close to the radar, demonstrating the possibility of radar based refractivity estimation in hilly terrain and non-homogeneous atmosphere with high spatial resolution.
Improved Joint Sparse Models for Hyperspectral Image Classification Based on a Novel Neighbour Selection Strategy
Authors: Gao Qishuo , Lim Samsung , Jia Xiuping
Published: June 2018

Joint sparse representation has been widely used for hyperspectral image classification in recent years, however, the equal weight assigned to each neighbouring pixel is less realistic, especially for the edge areas, and one fixed scale is not appropriate for the entire image extent. To overcome these problems, we propose an adaptive local neighbour selection strategy suitable for hyperspectral image classification. We also introduce a multi-level joint sparse model based on the proposed adaptive local neighbour selection strategy. This method can generate multiple joint sparse matrices on different levels based on the selected parameters, and the multi-level joint sparse optimization can be performed efficiently by a simultaneous orthogonal matching pursuit algorithm. Tests on three benchmark datasets show that the proposed method is superior to the conventional sparsity representation methods and the popular support vector machines.
Synthetic Aperture Radar Image Segmentation with Reaction Diffusion Level Set Evolution Equation in an Active Contour Model
Authors: Liu Jiaxing , Wen Xianbin , Meng Qingxia , Xu Haixia , Yuan Liming
Published: June 2018

This paper presents a method for synthetic aperture radar (SAR) image segmentation by draing upon a reaction–diffusion (RD) level set evolution (LSE) equation. The well-known RD theory consists of two main parts: reaction and diffusion terms. We first constructed the reaction term using an energy functional, which integrates the gamma statistical distribution with region–edge information from SAR images that can simultaneously suppress speckle noise and drive the active contour toward the object boundaries. Then, we used partial differential equation-based LSE to solve the proposed energy functional. Finally, a diffusion term was introduced into the LSE to ensure stability of the level set function and regularize the segmented region. The experimental results of both simulated and real SAR images showed that the proposed model has good robustness against a speckle noise as well as higher segmentation efficiency and accuracy than some existing models.
Fast and Automatic Data-Driven Thresholding for Inundation Mapping with Sentinel-2 Data
Authors: Kordelas Georgios A. , Manakos Ioannis , Aragonés David , Díaz-Delgado Ricardo , Bustamante Javier
Published: June 2018

Satellite data offer the opportunity for monitoring the temporal flooding dynamics of seasonal wetlands, a parameter that is essential for the ecosystem services these areas provide. This study introduces an unsupervised approach to estimate the extent of flooded areas in a satellite image relying on the physics of light interaction with water, vegetation and their combination. The approach detects automatically thresholds on the Short-Wave Infrared (SWIR) band and on a Modified-Normalized Difference Vegetation Index (MNDVI), derived from radiometrically-corrected Sentinel-2 data. Then, it combines them in a meaningful way based on a knowledge base coming out of an iterative trial and error process. Classes of interest concern water and non-water areas. The water class is comprised of the open-water and water-vegetation subclasses. In parallel, a supervised approach is implemented mainly for performance comparison reasons. The latter approach performs a random forest classification on a set of bands and indices extracted from Sentinel-2 data. The approaches are able to discriminate the water class in different types of wetlands (marshland, rice-paddies and temporary ponds) existing in the Doñana Biosphere Reserve study area, located in southwest Spain. Both unsupervised and supervised approaches are examined against validation data derived from Landsat satellite inundation time series maps, generated by the local administration and offered as an online service since 1983. Accuracy assessment metrics show that both approaches have similarly high classification performance (e.g., the combined kappa coefficient of the unsupervised and the supervised approach is 0.8827 and 0.9477, and the combined overall accuracy is 97.71% and 98.95, respectively). The unsupervised approach can be used by non-trained personnel with a potential for transferability to sites of, at least, similar characteristics.