bluuebunny/crossref_metadata_2025 huggingface dataset:
Internal Characteristics of Air-Supplied Plasma Synthetic Jet Actuator
Authors: Liu Rubing , Xue Shenghui , Wei Wentao , Lin Qi , Tang Kun
Published: February 2023

Conventional plasma synthetic jet actuators rely only on jet orifice for suction when functioning for long durations. A limited supplementary gas leads to jet velocity reduction and weakening of the flow control ability. Therefore, this study proposes an air-supplied actuator with a check valve externally connected to the cavity to improve its gas-supplying ability and jet performance. A quartz glass discharge chamber is developed to clarify the internal working mechanism of the air-supplied actuator. High-speed schlieren is employed to photograph the internal flow field of the discharge chamber. The results reveal that the inhalation airflow velocity of the jet orifice is doubled when the actuator is continuously working in the effective frequency band under the combined action of additional air supply from the check valve in the inhalation recovery stage. The gas pressure in the cavity is closer to the initial discharge state, discharge breakdown voltage is higher, discharge energy is stronger, and the process of gas expansion to generate a jet is less affected by the core defect of the heat source, thereby significantly increasing the jet velocity and saturation operating frequency of the actuator. The obtained results have important implications for the performance optimization of the air-supplied actuator.
Comparative Analysis of Flame Propagation and Flammability Limits of CH4/H2/Air Mixture with or without Nanosecond Plasma Discharges
Authors: Mehdi Ghazanfar , De Giorgi Maria Grazia , Bonuso Sara , Shah Zubair Ali , Cinieri Giacomo , Ficarella Antonio
Published: February 2023

This study investigates the kinetic modeling of CH4/H2/Air mixture with nanosecond pulse discharge (NSPD) by varying H2/CH4 ratios from 0 to 20% at ambient pressure and temperature. A validated version of the plasma and chemical kinetic mechanisms was used. Two numerical tools, ZDPlasKin and CHEMKIN, were combined to analyze the thermal and kinetic effects of NSPD on flame speed enhancement. The addition of H2 and plasma excitation increased flame speed. The highest improvement (35%) was seen with 20% H2 and 1.2 mJ plasma energy input at ϕ = 1. Without plasma discharge, a 20% H2 blend only improved flame speed by 14% compared to 100% CH4. The study found that lean conditions at low flame temperature resulted in significant improvement in flame speed. With 20% H2 and NSPD, flame speed reached 37 cm/s at flame temperature of 2040 K at ϕ = 0.8. Similar results were observed with 0% and 5% H2 and a flame temperature of 2200 K at ϕ = 1. Lowering the flame temperature reduced NOx emissions. Combining 20% H2 and NSPD also increased the flammability limit to ϕ = 0.35 at a flame temperature of 1350 K, allowing for self-sustained combustion even at low temperatures.
Study on Characteristics and Invulnerability of Airspace Sector Network Using Complex Network Theory
Authors: Liang Haijun , Zhang Shiyu , Kong Jianguo
Published: February 2023

The air traffic control (ATC) network’s airspace sector is a crucial component of air traffic management. The increasing demand for air transportation services has made limited airspace a significant challenge to sustainable and efficient air transport operations. To address the issue of traffic congestion and flight delays, improving the operational efficiency of ATC has been identified as a key strategy. A clear understanding of the characteristics of airspace sectors, which are the building blocks of ATC, is essential for optimizing air traffic management. In this research, a novel approach using complex network theory was applied to examine the features and invulnerability of the airspace sector network. We developed a model of the airspace sector network by treating air traffic control sectors as network nodes and the flow of air traffic between these sectors as edges. Network characteristics were analyzed using several metrics including degree, intensity, average path length, betweenness centrality, and clustering coefficient. The static invulnerability of the airspace sector network was evaluated through simulation, and the network efficiency and the size of the connected component were used to assess its invulnerability. A study was conducted in North China based on the ATC sector network. The findings of the study revealed that the sector network did not exhibit the traits of a small-world network model, characterized by short average path lengths and high clustering coefficients. The evaluation of network invulnerability showed that the network’s invulnerability varied depending on the attack strategy used. It was discovered that attacking sectors with high betweenness resulted in the most significant harm to network invulnerability, and betweenness centrality was considered to be a useful indicator for identifying critical sectors that require optimization.
Dynamic Features of Non-Return Mechanism in Hatch Door of Amphibious Aircraft
Authors: Wang Yanzhong , Dou Delong , Dong Xiaoyan
Published: February 2023

The non-return mechanism is the key device that plays the role of braking in the opening-and-closing electric actuator of an amphibious aircraft hatch door. The ball-and-socket contact pair, providing the pressing force, and the multi-disc friction pair, supplying the braking torque, are two core components in the non-return mechanism. In this paper, the dynamic model of the non-return mechanism is established considering the freedom of rotation-translation. Based on MATLAB/Simulink, the solution framework of the overall dynamic model is built. The dynamic response characteristics of the non-return mechanism in the process of reverse load braking, forward load braking, and continuous closing are analyzed. The braking time and the angular displacement of the output shaft in the braking phase have been presented. Compared with forward load braking, reverse load braking has a longer braking time and smaller angular displacement of the output shaft. In addition, the compaction function of the ball-and-socket contact pair is verified by experiment, and the influence of the cone angle, distribution radius of the ball socket, and the steel ball diameter on the pressing force has been discussed. This study provides a theoretical basis and parametric design platform for the design and optimization of non-return mechanisms, which can shorten the product development time.
Framework Development for Efficient Mission-Oriented Satellite System-Level Design
Authors: Kwon Kybeom , Min Seunghyun , Kim Jongbum , Lee Kwangwon
Published: February 2023

The space mission analysis and design process defines a space system at the system level to accomplish space mission objectives. Although the traditional process is well established and comprehensive through several years of experience, we propose a novel design process framework in this paper to aid the traditional process focusing on the following areas of improvement: (1) clarification of the direct connection between mission objectives and final system-level baseline design and requirements, (2) development of a comprehensive quantitative judgment criterion to evaluate various design alternatives, (3) derivation of system drivers and critical requirements after obtaining sufficient design knowledge based on the analysis of big data obtained from exploration of entire design space using an integrated design environment, and (4) system optimization even at the system level with a holistic perspective to guarantee that the baseline design meets the mission objectives. Examples of design steps in the proposed framework are characterizing stakeholder needs and engineering characteristics, building an integrated design environment, exploring and analyzing design space, optimizing system-level design, and elaborating mission utility to ensure an efficient mission-oriented design approach. The proposed framework is implemented in an example space mission involving quantum cryptographic communication. Accordingly, we demonstrate that the proposed framework provides an efficient mission-oriented satellite system-level baseline design.
Control of Electromagnetic Formation Flight of Two Satellites in Low Earth Orbits
Authors: Song Yingying , Zhou Qingrui , Chen Qingwei
Published: February 2023

Electromagnetic formation flight uses the electromagnetic interaction between satellites to provide maneuver control for formation satellites, with the advantages of no propellant consumption, long life, and high flexibility. However, high-precision control for electromagnetic formation flight is challenging because of the nonlinear and coupling characteristics of the dynamics, optimal assignment of magnetic dipoles, model uncertainties, and the angular momentum management issues caused by the geomagnetic field. This paper studies the 6-DOF control problem of two-satellite electromagnetic formation flight in low-Earth orbit. A new electromagnetic frame is introduced to promote the decoupling of the translation dynamics model and the electromagnetic model. The electromagnetic model can be expressed as a simple two-dimensional model in this electromagnetic frame. The proposed electromagnetic force envelope diagram can intuitively show the relationship between electromagnetic force and magnetic dipoles, providing practical guidance for dipole assignment. The frequency division multiplexing method is designed for angular momentum management considering the effect of the earth’s magnetic field on the electromagnetic satellites, and the active disturbance rejection control method is used to solve the 6-DOF stability problem with external disturbance and model uncertainties. Numerical simulation verifies the effectiveness of the proposed control method and angular momentum management strategy.
Physiological Changes and Yield Components of Safflower (Carthamus tinctorius L.) Lines as a Function of Water Deficit and Recovery in the Flowering Phase
Authors: de Almeida Silva Marcelo , Santos Hariane Luiz , de Sousa Ferreira Lusiane , Silva Dayane Mércia Ribeiro , dos Santos Jania Claudia Camilo , de Almeida Prado Bortolheiro Fernanda Pacheco
Published: February 2023

Since climate changes have caused water restrictions, safflower stands out as an alternative crop due to its adaptability to restrictive soil and climate conditions. Thus, this research aimed to evaluate the physiological and yield performance of four safflower lines (IMA 02, IMA 04, IMA 14, and IMA 21) under two water regimes [without water deficiency—around 22% soil moisture content—100% of field capacity (FC); and with water deficiency—50% of FC]. The water regimes were imposed for 30 days during the flowering phase, followed by rehydration for 20 days. Water deficiency decreased relative water content, water potential, photosynthetic pigment contents, photosynthetic performance, maximum variable and potential quantum yield of PSII, electron transport rate, and photochemical quenching. In contrast, it increased electrolyte leakage, water use efficiency, and non-photochemical quenching. The decreases in photochemical efficiency and photosynthetic performance as a function of water deficiency caused reductions in the number of capitula, 100-grain mass, and harvest index, with more significant reductions in IMA 02, which was considered susceptible to soil water changes. IMA 04, IMA 14, and IMA 21 were considered tolerant because their physiological variables and yield components were less affected by water restriction, and they also showed recovery after rehydration compared to IMA 02. Thus, these lines can be recommended for commercial use, and safflower breeding programs aiming to select superior genotypes under drought conditions.
Utilization of UAV Remote Sensing in Plant-Based Field Experiments: A Case Study of the Evaluation of LAI in a Small-Scale Sweetcorn Experiment
Authors: Jung Hyunjin , Tajima Ryosuke , Ye Rongling , Hashimoto Naoyuki , Yang Yi , Yamamoto Shuhei , Homma Koki
Published: February 2023

In crop production, which is largely dependent on environmental conditions, various attempts at environmental or social changes have been highlighted, and many field experiments are needed for them. However, since field experiments in agricultural production are constrained by high labor and time consumption, alternative methods to respond to these constraints are required. In this study, to establish a new method for application to field experiments, we proposed the evaluation of the leaf area index (LAI) of all individual plants in an experimental sweetcorn field using an unmanned aerial vehicle (UAV). Small-scale field experiments were conducted over two years. In the first year, the nitrogen fertilizer level was changed, and the plant density and additional nitrogen fertilizer application time were changed in the next year. Three vegetation indices (VIs), namely, the normalized difference vegetation index (NDVI), enhanced vegetation index 2 (EVI2), and simple ratio (SR), were validated to quantify the LAI estimation using a UAV for individual plants. For the evaluation of the individual plants, we used a plant-based method, which created all of the plant buffers based on the points of existing plants and the plant distance. To confirm the impact of the method, we additionally demonstrated the relationship between the LAI and yield, the results of statical analyses, and the difference of the center and the border of the field. Among the three VIs, index SR was found the most promising in the estimation of the LAI of the individual sweetcorn plants, providing the strongest correlation of yield with SR. Because a lot of data were obtained using the plant-based method, the statical differences in the LAI and yield were more easily detected for the plant density and fertilizer treatments. Furthermore, interesting differences between the center and the border of the field were found. These results indicate the availability and impact of plant-based evaluations using UAVs in near future field experiments.
Cost-Effectiveness of Acaricide Application Methods against Heartwater Disease in South Africa
Authors: van den Heever Mario J. J. , Lombard Willem A. , Bahta Yonas T. , Maré Frikkie A.
Published: February 2023

In an economic sense, heartwater disease is the most important tick-borne disease faced by South African livestock farmers. Methods to control the disease vary among farmers, and the preferred method depends on the prevalence of the disease in a specific area. The cost-effectiveness of different acaricide prevention methods against heartwater disease has not yet been determined amongst South African livestock producers. The study’s objective was to determine the cost-effectiveness of acaricide prevention methods used against heartwater disease in South Africa. Data used for this study were collected through a survey and structured questionnaires from 272 commercial livestock farmers in South Africa. Cost-effectiveness analyses were done on the spray, plunge, and pour-on acaricide application methods. For sheep and cattle, the plunge method proved to be the most cost-effective in all provinces of South Africa. In goats, pour-on acaricide application was the most cost-effective. The study recommends that extension activities provide farmers with information with which to choose the most appropriate acaricide application for the effective and sustainable control of heartwater disease.
The Effect of Information Acquisition Ability on Farmers’ Agricultural Productive Service Behavior: An Empirical Analysis of Corn Farmers in Northeast China
Authors: Yue Shoumin , Xue Ying , Lyu Jie , Wang Kangkang
Published: February 2023

Agricultural productive services are an important means to achieve effective allocation of regional resources and play an important role in ensuring food security and improving farmers’ welfare. However, the development process of agricultural productive services still faces problems such as large differences in service levels in different segments and low participation rates in the full service. In order to investigate the influential paths of the low participation rate of farmers in the full-service process, this study takes maize farmers in northeast China as the research object. Based on 937 survey data from six cities in three northeastern provinces, we used the Item Response Theory (IRT) model to measure farmers’ information acquisition ability and constructed the Heckman two-stage model and the IV-Heckman model to analyze the logical framework of “information acquisition ability—farmers’ choice of productive agricultural services”. The main findings are as follows: firstly, the more channels there are, the stronger the farmers’ channel internalities; the higher the degree of channel differentiation, the stronger the farmers’ channel internalities. Second, after addressing the sample selection bias and endogeneity, there is a small rise in the facilitation effect of information acquisition ability on farmers’ productive agricultural service behavior. Third, this facilitation effect is achieved through farmers’ perceived usefulness of productive agricultural services, and the mediating effect of perceived ease of use is not significant. Therefore, fostering farmers’ self-perceptions and optimizing information delivery strategies are effective ways to promote farmers’ choice of agricultural productive services and to facilitate the modernization of Chinese agriculture. In general, this study helps to reveal the theoretical mechanism of farmers’ information asymmetry, and provides empirical evidence for how to promote the development of agricultural productive services.
Improving the Use of White Lupine in the Laying Quail Feeding by Enzymes Addition: Effects on Productive Performances, Digestion, Blood Biochemical Indices and Eggs Quality
Authors: Struți Dănuț Ioan , Mierlita Daniel , Bunea Andrea
Published: February 2023

Lupine seeds are a valuable nutritive source for animal feeding, but for poultry nutrition, the content in crude fiber and non-starch polysaccharides (NSP) have an antinutritional factor. The aim of this research was to highlight the effect of partial soybean meal replacement with L. albus seeds and enzyme addition in the laying quail diets on productive performance, digestion, blood biochemical indices and egg quality. A total of 210 homogenous female Japanese quails (Coturnix japonica) at 24 week of age were randomly assigned to 6 dietary treatments, with the standard diet based on soybean meal unsupplemented (−) and supplemented with enzyme (+) (S−/S+) and the experimental diets on which the soybean meal was based partially substituted by including lupine in the amount of 200 g/kg and 250 g/kg, unsupplemented and supplemented with enzymes (L20−/L20+; L25−/L25+).The use of enzymes in the lupine-based diets allowed increasing the proportion of lupine in the diet of laying quails by up to 25% (% of feed) without changing egg production, egg weight, feed conversion rate and physical–chemical quality parameters of the eggs. In addition, the use of lupine (−/+) improved (p < 0.001) the carotenoid content of the egg yolk, as well as the quality of the yolk fats by decreasing the cholesterol content and the level of fatty acids (FA) with an atherogenic effect, in favor of omega-3 FA. Enzyme supplementation of the lupine-based diets had a negative effect on the health lipid indices of the fats in the yolk (ratio of the hypocholesterolemic/Hypercholesterolemic FA—h/H, atherogenic index—AI, thrombogenic index—TI and health promotion index—HPI). The use of exogenous enzymes increased the nutrients’ efficiency of the quails’ feed, which is supported by the improvement of the blood metabolic indices and a decrease of intestinal digesta viscosity and feces moisture. In conclusion, white lupine can be used up to 25% in the laying quail feed in association with specific enzymes without affecting the productive performance and egg quality; moreover, lupine use has improved the quality of the eggs, increasing humans’ health.
Ions Transport in Seasonal Frozen Farmland Soil and Its Effect on Soil Salinization Chemical Properties
Authors: Liu Yawen , Wu Jingwei , Zhao Hang , Li Changsen , Mao Jun , Zhang Rui , Liu Jie , Zhao Qiang
Published: February 2023

The salinization of farmland soil is exacerbated during the freeze–thaw (FT) process, endangering agricultural production. The change of soil salt ions results in the formation and development of soil salinization. The objectives of this study were to investigate the migration characteristics of salt ions during the FT process, identify the effects of inconsistencies in ions transport on the development of soil salinization chemical properties. A six-month field observation was conducted from November 2020 to April 2021 in the Hetao Irrigation District, China, a typical seasonally frozen soil area affected by salinization. Soil salt ions, soil moisture content (SMC), soil temperature, and pH were measured. Soil salt content (SSC), sodium adsorption ratio (SAR) were calculated. The ions accumulated in the frozen soil layer during the freezing period in the order of Cl− > Mg2+ > Ca2+ > Na+ > SO42−, and accumulated in the topsoil during the thawing period in the order of Cl− > Na+ > Mg2+ > Ca2+ > SO42−, while the change in HCO3− was mostly the opposite. The FT process changed the main salt anions from sulfate to chloride. After the FT process, the topsoil was endangered by high salinization, excess Cl− toxicity, and a potential alkalization threat. This study has great guiding significance for the management and control of soil salinization before spring sowing in saline areas.
Harvest Aids Applied at Appropriate Time Could Reduce the Damage to Cotton Yield and Fiber Quality
Authors: Zhang Qipeng , Sun Yuanyuan , Luo Dan , Li Peisong , Liu Taofen , Xiang Dao , Zhang Yali , Yang Mingfeng , Gou Ling , Tian Jingshan , Zhang Wangfeng
Published: February 2023

The application of harvest aids is an important prerequisite for the mechanical harvesting of cotton that can effectively reduce the impurity content and improve the picking rate and operating efficiency of machine-picked cotton. However, determining the appropriate spraying time of harvest aids to achieve the synergistic improvement of cotton boll weight and fiber quality is still unclear. In this study, the damage of harvest aids to cotton boll weight and fiber quality as well as its quantitative relationship to cotton boll age were studied through testing different harvest aid compounds and spraying times. The spraying of harvest aids significantly shortened the boll growth period of cotton by 3.60–6.45 d, and concentrated boll opening was beneficial to cotton mechanical harvesting. The boll weight of immature cotton was significantly decreased by 0.63–1.12 g; the fiber strength was significantly decreased by 2.48–2.77 cN·tex−1, and the micronaire value deteriorated. The negative effect on the boll weight and fiber quality was aggravated by the decrease in the ratio of boll age to boll period (Rd/b) during the harvest aid spraying time. When the fiber strength damage was controlled at 1%, the spraying time Rd/b of the harvest aids was 0.77–0.82, and the boll weight loss was also controlled at 5%. Therefore, it is recommended that an Rd/b of 0.77–0.82 be used to balance the contradiction between cotton yield and fiber quality under harvest aid application.
Sustainable Agriculture: Rare-Actinomycetes to the Rescue
Authors: Oyedoh Oghoye P. , Yang Wei , Dhanasekaran Dharumadurai , Santoyo Gustavo , Glick Bernard R. , Babalola Olubukola O.
Published: February 2023

The failure of sustainable and agricultural intensifications in saving the ecosystem/public health has caused a paradigm shift to microbiome resource engineering through sustainable approaches. As agricultural intensification systems prioritize synthetic input applications over environmental health, sustainable intensification fails to define the end point of intensification, giving room for the application of “intensification” over “sustainability” to suit farmers’ needs. However, sustainable agricultural practices through microbiome resource services have been well harnessed and appreciated for their significant role in plant health and disease management due to their ability to secret agroactive metabolites with notable functionalities in a cooperative manner or as bioinoculants. The complexity of a cooperative microbiome and the uncontrollable nature of its numerous influencing parameters as well as the non-specificity associated with bioinoculant application, results in the direct utilization of agroactive compounds to obtain greater preventive efficiency. In this regard, the known bacterial trove has been seriously ransacked, yet there exists an inexhaustible bank of unknown compounds, which are conserved in Actinomycetes. However, the rare Actinomycetes group has received less attention than other plant growth-promoting bacteria; thus, the possibility exists that the Actinomycetes may encode novel useful metabolites. To unravel the possible uses of these metabolites for phytoprotection, smart culture-based techniques and genometabolomics technology have been applied. Hence the aim of this review is to express the sustainable nature of agro-antibiotics or biopesticide from these bacterial resources for the resolution of phytopathogenic havoc that reduces crop productivity.
Karrikin1 Enhances Drought Tolerance in Creeping Bentgrass in Association with Antioxidative Protection and Regulation of Stress-Responsive Gene Expression
Authors: Tan Zhen-Zhen , Wang Yi-Ting , Zhang Xia-Xiang , Jiang Heng-Yue , Li Ya , Zhuang Li-Li , Yu Jing-Jin , Yang Zhi-Min
Published: February 2023

Karrikins are active components of smoke that can promote seed germination and regulate seedling morphogenesis. However, the role of karrikins as alleviators of abiotic stress remains largely elusive. In this study, we examined whether exogenous application of karrikin1 (KAR1) might improve drought tolerance in creeping bentgrass (Agrostis stolonifera cv. PennA4), and investigated the underlying mechanism. We found that exogenous application of 100 nM KAR1 enhanced drought tolerance in creeping bentgrass, as manifested by significant increases in leaf relative water content, efficiency of photosystem II, leaf chlorophyll content, proline content, and membrane stability, as well as significantly enhanced activities of antioxidant enzymes. RT–PCR analysis indicated that improved drought stress tolerance by application of KAR1 might be related to upregulation expression of karrikin-responsive genes (KAI2, MAX2 and AFL1), transcription factors (ABF3, bHLH148, MYB13 and DREB2A), antioxidant defense genes (Cu/Zn-SOD, APX2, CAT1, and POD2), and downregulation expression of chlorophyll-degradation genes (PPH and Chl-PRX). These findings suggest that KAR1 may promote the drought tolerance of creeping bentgrass by activating karrikin-responsive genes and transcription factors, enhancing proline accumulation and antioxidant capacity, and suppressing leaf senescence under prolonged drought stress.
Epidemiology of Xanthomonas euvesicatoria in Tokat Province
Authors: Kayaaslan Zeliha , Belgüzar Sabriye , Yanar Yusuf , Mirik Mustafa
Published: February 2023

This study was carried out to determine the life span of Xanthomonas euvesicatoria (Xeu) in seeds, soil and plant residues and the first inoculum source that cause the onset of the disease. The study was carried out in the province of Tokat. Rifampicin-resistant Xeu isolate was inoculated into soil, seeds and plant residues. As a result of the studies, it was determined that Xeu remained viable in inoculated seeds until the 220th day (7 months), and lived in the soil for 105 days in summer and 14 days in winter. In plant residues, it was determined that the the life span in summer period was 60 days at 0 cm depth, 30 days at 20 cm depth; 30 days at 0 cm depth and 15 days at 20 cm depth in that of winter period. According to the results of this study, it was determined that the Xeu did not survive for a long time in plant residues and soil in Tokat province and thus was not carried to the next year, on the other hand the seed was the most important source of inoculum.
Drop Size Measurement Techniques for Agricultural Sprays:A State-of-The-Art Review
Authors: Privitera Salvatore , Manetto Giuseppe , Pascuzzi Simone , Pessina Domenico , Cerruto Emanuele
Published: February 2023

Plant protection control based on the spray application of plant protection products is a very complex task depending on a series of factors, among which droplet size is the most influential for deposition and pesticide effectiveness. In fact, the adoption of the correct droplet size can ensure that the required dose reaches the target area and is not wasted, minimizes the off-target losses due to evaporation, drift and run-off and, at the same time, enhances the operator’s safety in terms of inhalation, ingestion and dermal exposure. In this paper, after defining some mean characteristic diameters helpful for a description of a drop population and focusing on the main drop size distribution functions for the statistical characterization of sprays, a critical analysis of known methods, both intrusive and non-intrusive, for drop size measurement is carried out by reviewing the literature. Among intrusive methods, the liquid immersion method and the use of water-sensitive papers are discussed, whereas, among non-intrusive methods, laser-based systems (laser diffraction, phase Doppler particle analysis) and high-speed imaging (shadowgrapy) are presented. Both types of method, intrusive and non-intrusive, can be used in machine-learning-based approaches exploiting regression techniques and neural network analysis.
Plant Composition and Feed Value of First Cut Permanent Meadows
Authors: Dal Prà Aldo , Davolio Roberto , Immovilli Alessandra , Burato Andrea , Ronga Domenico
Published: February 2023

Permanent grasslands represent the main terrestrial ecosystem and serve as an important global reservoir of biodiversity, providing a wide range of benefits to humans and ecosystems. The effects of environment on permanent meadows (in our survey, they were centuries-old meadows that had not been plowed, mowed, or fertilized with manure) production have been adequately investigated in literature. However, plant species composition impact on potential feed value of first cut has still to be understood, in particular regarding different agronomic management. Our field trial was carried out in five farms, in a territory involved in the value chain of the Parmigiano Reggiano PDO (Val d’Enza, Northern Italy), over a two-year period (2017–2018). Differences in botanical composition, biomass, and Pastoral Value index (PV), which synthesizes grassland yield and nutritional parameters, were investigated in depth. The herbage dry matter (DM) yield was affected by year, farm, and their interaction factors. Its highest value across the two years was recorded in farm 5 (11.7 tons of DM ha−1), which applied the highest rate of nitrogen fertilization. The botanical composition of the first cut has favored the presence of both Poaceae and ‘other species’ (each one around 40 plants per transect) compared to Fabaceae (seven plants per transect). However, higher numbers of Fabaceae plants (13 and 10) plausibly determined increases in PV in farms 3 and 5 (56.4 and 58.7, respectively). Although differences were observed among the most important nutritional parameters of grassland (crude protein, digestible and undigested neutral detergent fiber contents), suitable net energy for lactation (NEL) values for feeding lactating cows were always recorded during the two years of survey. The present study provides a contribution of knowledge on how the botanical composition of permanent meadows may affect their potential nutritive value as fresh herbage for feeding dairy cows. Considering these results, the agronomic management should seek a level of plant biodiversity that at the same time might guarantee satisfactory yield and feed value, also in a context of climate change.
Three Cycles of Continuous Propagation of a Severe PSTVd Strain NicTr-3 in Solanum lycopersicum cv. Rutgers Resulted in Its Attenuation and Very Mild Disease Symptoms in Potato
Authors: Kochetov Alex V. , Shmakov Nikolay , Afonnikov Dmitry A. , Vasiliev Gennady V. , Shatskaya Natalja V. , Egorova Anastasiya A. , Mironenko Nina V. , Lashina Nina M. , Khiutti Alexander V. , Afanasenko Olga S.
Published: February 2023

Potato spindle tuber viroid (PSTVd) is a small infectious non-coding circular RNA causing diseases of important crops, including tomato and potato. The symptoms vary from mild to severe depending on the viroid strain, the host plant variety, and the environmental conditions. The molecular mechanisms underlying the development of particular disease phenotypes remain elusive. The PSTVd strain NicTr-3 causes severe disease symptoms on Solanum lycopersicum cv. Rutgers and Solanum tuberosum cv. Colomba. It was found that after three cycles of propagation in tomato cv. Rutgers, infection of potato cv. Colomba with NicTr-3 resulted in very mild symptoms including smaller size of tubers and delayed leaf senescence. Sequence analysis of PSTVd clones isolated from tomato inoculum revealed the presence of mutated variants of genomic RNA. Transcriptome analysis carried out on leaves showed a considerable difference between infected and healthy plants at 14 dpi and 30 dpi. Interestingly, the response of potato plants to the attenuated PSTVd strain revealed a large number of DEGs associated with initiation of dormancy with a considerable increase in the vegetation period. The second vegetative generation (tuber-derived plantlets from control healthy and PSTVd-infected plants) was characterized by similar phenotypes and transcriptomes. Thus, here we describe a case of attenuation of a severe PSTVd strain after continuous propagation in tomato. It would be of interest to consider the attenuated viroid strains as a potential biocontrol agent or vaccine against this type of pathogenic RNA.
How Much Impact Has the Cover Crop Mulch in Mitigating Soil Compaction?—A Field Study in North Italy
Authors: Benetti Marco , Liu Kaihua , Guerrini Lorenzo , Gasparini Franco , Peruzzi Andrea , Sartori Luigi
Published: February 2023

Soil compaction was largely studied in different scenarios with laboratory and field scale experiments, with various soil conditions and traffic intensities. However, a detailed analysis to better understand the protective role of plant residues or cover crop mulch is still required. A field test was conducted in Northeast Italy aiming to fill this gap. Rye was chosen as a winter cover crop, and growth on a controlled traffic random block experimental field. Four different cover crop mulch treatments were compared to study the effects of root systems: roller crimper, flail mower, bare soil control and harvested biomass control. Four different traffic intensities were used to evaluate the multiple passages with 0, 1, 3, 5 traffic events. During traffic events, the mean normal stress was measured. Penetration resistance was then evaluated after trafficking and soil samples were collected. The obtained results showed a 19.3% cone index increase in bare soil compared to flail mower treatment after the first traffic event, while low differences were found in harvested biomass bulk density during the first and third traffic events. Moreover, mean normal stress increased 16.5% on harvested biomass treatment compared to the flail mower. These findings highlight that the cover crop maintains a lower soil penetration resistance during compaction events, helping the subsequent field operations. Furthermore, roller crimper and flail mower cover crop termination impact soil bearing capacity differently due to different soil moisture content. However, the results showed a low contribution of cover crop mulch on mitigating soil compaction effects during the experiment.
Effect of Quebracho Tannin (Schinopsis quebracho-colorado (Schltdl.) F.A. Barkley and T. Meyer) on Silage Nutritive Value, Ergovaline Concentration, and Fermentation Parameters of Tall Fescue (Schedonorus arundinaceus (Shreb.) Dumort) with Two Dry-Matter Levels
Authors: Grote Ally J. , Nieman Christine C. , Thomas Jr. Ivan R. , Coffey Kenneth P. , Muir James P. , Klotz James L.
Published: February 2023

Tall fescue (Schedonorus arundinaceus (Shreb.) Dumort) is a cool-season forage grown in the mid-south United States of America that has the potential for spring silage. Ergovaline produced by the fungal endophyte Neotyphodium coenophialum is preserved in tall fescue silage and can induce tall fescue toxicosis in livestock. Condensed tannins, such as quebracho (Schinopsis quebracho-colorado (Schltdl.) F.A. Barkley and T. Meyer) extract, can bind to the nitrogenous components of ergovaline, rendering it ineffective in the ruminant. Quebracho tannin (QT) can also bind to crude protein, reducing its conversion to ammonia. Quebracho tannin was either not added (0QT) or added at 10 (10QT) or 20 (20QT) g/kg of dry matter (DM) of silage to chopped endophyte-infected tall fescue of 670 g/kg of moisture (high moisture, HM) and 440 g/kg of moisture (low moisture, LM). A moisture × QT interaction affected the final pH (p = 0.02), with the lowest pH observed in HM silages with 0QT and 10QT. The ergovaline concentrations were not significantly different (p = 0.19) among the post-ensiled forages. Ammonia concentrations (g/kg DM) were affected by a moisture × QT interaction (p = 0.05), with greater concentrations observed in HM 0QT compared with HM 10QT, HM 20QT, and LM 20QT. Lactic acid concentrations (g/kg DM) decreased (p = 0.05) with the addition of QT. The moisture × QT interaction (p = 0.02) resulted in higher concentrations of acetic acid in the HM silages with 0QT and 20QT compared to the LM silage with 20QT. The total acid concentrations (g/kg DM) were higher (p < 0.01) at HM but did not differ (p = 0.54) across the QT concentrations. Ensiling tall fescue with quebracho tannin did not reduce the ergovaline concentrations, although proteolysis was reduced at the inclusion of 10 g/kg of QT in the HM silages and 20 g/kg of QT in the LM silages. The results indicate that QT as a tall fescue silage additive showed promise for modifying silage characteristics but it did not reduce ergovaline concentrations at the low QT levels used in this study.
Enrichment of Brain n-3 Docosapentaenoic Acid (DPA) and Retinal n-3 Eicosapentaenoic Acid (EPA) in Lambs Fed Nannochloropsis oceanica Microalga
Authors: Vítor Ana C. M. , Correia Jorge J. , Alves Susana P. , Bessa Rui J. B.
Published: February 2023

Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have special physiological functions in both brain and retinal tissues that are related to the modulation of inflammatory processes and direct effects on neuronal membrane fluidity, impacting mental and visual health. Among them, the long-chain (LC) n-3 PUFAs, as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are of special importance. Scarce data are available about the fatty acid (FA) composition of the ruminant brain in response to dietary intervention. However, we decided to examine the brain and retina FA composition of lambs supplemented with an EPA-rich microalga feed for 21 days, as it is known that despite the extensive biohydrogenation of dietary PUFAs in the rumen, ruminants can selectively accumulate some n-3 LC-PUFAs in their brain and retinal tissues. Twenty-eight male lambs were fed a control diet, or the same diet further supplemented with Nannochloropsis sp. microalga. Their brains and retina were collected for FA characterization. Overall, the brain FA profile remained unchanged, with little alteration in omega-3 docosapentaenoic acid (DPA) enhancement in both the hippocampus and prefrontal cortex. Retinal tissues were particularly responsive to the dietary intervention, with a 4.5-fold enhancement of EPA in the freeze-dried-fed lambs compared with the control lambs. We conclude that retinal tissues are sensitive to short-term n-3 PUFA supplementation in lambs.
Effect of Metabolizable Protein Supply on Milk Performance, Ruminal Fermentation, Apparent Total-Tract Digestibility, Energy and Nitrogen Utilization, and Enteric Methane Production of Ayrshire and Holstein Cows
Authors: Benchaar Chaouki , Hassanat Fadi , Beauchemin Karen A. , Ouellet Daniel R. , Lapierre Hélène , Côrtes Cristiano
Published: February 2023

In North America, the nutrient requirements of dairy cattle are predicted using the Cornell Net Carbohydrate and Protein System (CNCPS) or the National Research Council (NRC). As Holstein is the most predominant dairy cattle breed, these models were developed based on the phenotypic, physiological, and genetic characteristics of this breed. However, these models may not be appropriate to predict the nutrient requirements of other breeds, such as Ayrshire, that are phenotypically and genetically different from Holstein. The objective of this study was to evaluate the effects of increasing the metabolizable protein (MP) supply using CNCPS on milk performance, ruminal fermentation, apparent total-tract digestibility, energy and N utilization, and enteric methane production in Ayrshire vs. Holstein lactating dairy cows. Eighteen (nine Ayrshire; nine Holstein) lactating cows were used in a replicated 3 × 3 Latin square design (35-d periods) and fed diets formulated to meet 85%, 100%, or 115% of MP daily requirement. Except for milk production, no breed × MP supply interaction was observed for the response variables. Dry matter intake (DMI) and the yields of energy-corrected milk (ECM), fat, and protein were less (p < 0.01) in Ayrshire vs. Holstein cows. However, feed efficiency and N use efficiency for milk production did not differ between the two breeds, averaging 1.75 kg ECM/kg DMI and 33.7 g milk N/100 g N intake, respectively. Methane yield and intensity and urinary N also did not differ between the two breeds, averaging 18.8 g CH4 /kg DMI, 10.8 g CH4 /kg ECM, and 27.6 g N/100 g N intake, respectively. Yields of ECM and milk protein increased (p ≤ 0.01) with increasing MP supply from 85% to 100% but no or small increases occurred when MP supply increased from 100 to 115%. Feed efficiency increased linearly with an increasing MP supply. Nitrogen use efficiency (g N milk/100g N intake) decreased linearly (by up to 5.4 percentage units, (p < 0.01) whereas urinary N excretion (g/d or g/100 g N intake) increased linearly (p < 0.01) with an increasing MP supply. Methane yield and emission intensity were not affected by MP supply. This study shows that feed efficiency, N use efficiency, CH4 (yield and intensity), and urinary N losses did not differ between Ayrshire and Holstein cows. Energy-corrected milk yield and feed efficiency increased, but N use efficiency decreased and urinary N losses increased with increasing dietary MP supply regardless of breed. Ayrshire and Holstein breeds responded similarly to increasing MP levels in the diet.
Lessons Learned for Pre-Emptive Capture Management as a Tool for Wildlife Conservation during Oil Spills and Eradication Events
Authors: Chilvers B. Louise , McClelland Pete J.
Published: February 2023

Pre-emptive capture or translocation of wildlife during oil spills and prior to pest eradication poison applications are very specific conservation goals within the field of conservation translocation/reintroduction. Protection of wildlife from contamination events occurs during either planned operations such as pest eradication poison applications, or unplanned events such as pollution or oil spills. The aim in both incidences is to protect at-risk wildlife species, ensuring the survival of a threatened regional population or entire species, by excluding wildlife from entering affected areas and therefore preventing impacts on the protected wildlife. If pre-emptive capture does not occur, wildlife may unintentionally be affected and could either die or will need capture, cleaning, and/or medical care and rehabilitation before being released back into a cleared environment. This paper reviews information from pre-emptive captures and translocations of threatened wildlife undertaken during past oil spills and island pest eradications, to assess criteria for species captured, techniques used, outcomes of responses, and lessons learned. From these case studies, the considerations and planning needs for pre-emptive capture are described and recommendations made to allow better use and preparedness for pre-emptive capture as a preventative wildlife conservation tool.
Laparoscopically Assisted Percutaneous Inguinal Ring Closure for Resolution of Inguinal/Scrotal Hernias in Rams: Cadaveric Study and Three Cases Report
Authors: Vázquez Francisco J. , Romero Antonio , Fuente Sara , Barrachina Laura , Vitoria Arantza
Published: February 2023

The aim of this study is to evaluate a laparoscopically assisted percutaneous suture (LAPS) procedure to treat inguinal hernia (IH) while preserving testicles in rams. An ex vivo experiment with six ram cadavers and a report of three clinical cases are discussed. In cadavers, both internal inguinal rings (IIR) were partially closed by LAPS. Two LAPS methods were tested: (1) using a laparoscopic portal closure device and (2) using a suture loop inserted through needles in each IIR. After each procedure, the closure was laparoscopically evaluated and the number of U- sutures was recorded. The procedure was also performed on three client-owned rams with unilateral non-strangulated IH and the occurrence of re-herniation was followed up. In cadavers, LAPS of the IIRs could be easily and satisfactorily performed with either of the two systems, requiring one to three U-sutures per IIR. No differences were observed between the two surgical procedures. In two clinical cases, the procedure was successfully performed without reoccurrence of herniation or alterations in reproductive behavior in the following 3 and 6 months. In the third case, the hernia was reduced but a retroperitoneal emphysema during laparoscopy prevented hernioplasty and the animal herniated again. In conclusion, LAPS of IIR can be used as a simple and feasible treatment to preserve testicles in rams with IH.
Goose Meat as a Source of Dietary Manganese—A Systematic Review
Authors: Goluch Zuzanna , Haraf Gabriela
Published: February 2023

Manganese is a trace element with essential physiological functions that should be supplied to animals and humans through diet. Goose meat is prevalent in many regions of the world. Therefore, the aim of the study was a systematic review (PRISMA statement, 1980–2022) of the content of Mn in raw and cooked goose meat and their relation to the recommended intake at the level of adequate intake (AI) and the nutrient reference values-requirements (NRV-R). The literature analysis shows that the content of Mn in goose meat depends on the breed, type of muscles, the presence of skin, and the cooking method used. AI level recommendations for Mn intake range from 0.003 to 5.50 mg/day, depending on the country, age, and gender. Consumption by adults (regardless of sex) of 100 g of domestic or wild goose meat covers the daily AI per Mn in various percentages, depending on the type of muscles (more Mn in leg muscles), presence of skin (more Mn in skinless muscles), and thermal treatment (pan fried with oil, grilled, and cooked meat contains more). Placing information on the Mn content in goose meat and the percentage of NRV-R on the packaging may be valuable information for the consumer in making food choices to diversify the diet. There are few studies on the content of Mn in goose meat. Therefore, it is reasonable to conduct research in this area.