Exhaled volatilome analysis as a useful tool to discriminate asthma with other coexisting atopic diseases in women of childbearing age
Authors: Sola-Martínez Rosa A. , Lozano-Terol Gema , Gallego-Jara Julia , Morales Eva , Cantero-Cano Esther , Sanchez-Solis Manuel , García-Marcos Luis , Jiménez-Guerrero Pedro , Noguera-Velasco José A. , Cánovas Díaz Manuel , de Diego Puente Teresa ,   the NELA study group, Candel-Torralba M. E. , Garcia-Marcos L. , Gimenez-Banon M. J. , Martinez-Torres A. , Morales E. , Perez-Fernandez V. , Sanchez-Solis M. , Nieto A. , Prieto-Sanchez M. T. , Sanchez-Ferrer M. , Fernandez-Palacios L. , Gomez-Gomez V. P. , Martinez-Gracia C. , Peso-Echarri P. , Ros-Berruezo G. , Santaella-Pascual M. , Gazquez A. , Larque E. , Pastor-Fajardo M. T. , Sanchez-Campillo M. , Serrano-Munuera A. , Zornoza-Moreno M. , Jimenez-Guerrero P. , Adomnei E. , Arense-Gonzalo J. J. , Mendiola J. , Navarro-Lafuente F. , Torres-Cantero A. M. , Salvador-Garcia C. , Segovia-Hernández M. , Yagüe-Guirao G. , Valero-Guillén P. L. , Aviles-Plaza F. V. , Cabezas-Herrera J. , Martinez-Lopez A. , Martinez-Villanueva M. , Noguera-Velasco J. A. , Cantero-Cano E. , Franco-Garcia A. , Garcia-Serna A. M. , Hernandez-Caselles T. , Martin-Orozco E. , Norte-Muñoz M. , Cánovas Díaz M. , de Diego Puente T. , Pastor J. M. , Sola-Martínez R. A. , Esteban-Gil A. , Fernández-Breis J. T. , Alcántara M. V. , Hernández S. , López-Soler C. 
Published: July 2021

The prevalence of asthma is considerably high among women of childbearing age. Most asthmatic women also often have other atopic disorders. Therefore, the differentiation between patients with atopic diseases without asthma and asthmatics with coexisting diseases is essential to avoid underdiagnosis of asthma and to design strategies to reduce symptom severity and improve quality of life of patients. Hence, we aimed for the first time to conduct an analysis of volatile organic compounds in exhaled breath of women of childbearing age as a new approach to discriminate between asthmatics with other coexisting atopic diseases and non-asthmatics (with or without atopic diseases), which could be a helpful tool for more accurate asthma detection and monitoring using a noninvasive technique in the near future. In this study, exhaled air samples of 336 women (training set (n = 211) and validation set (n = 125)) were collected and analyzed by thermal desorption coupled with gas chromatography-mass spectrometry. ASCA (ANOVA (analysis of variance) simultaneous component analysis) and LASSO + LS (least absolute shrinkage and selection operator + logistic regression) were employed for data analysis. Fifteen statistically significant models (p-value < 0.05 in permutation tests) that discriminated asthma with other coexisting atopic diseases in women of childbearing age were generated. Acetone, 2-ethyl-1-hexanol and a tetrahydroisoquinoline derivative were selected as discriminants of asthma with other coexisting atopic diseases. In addition, carbon disulfide, a tetrahydroisoquinoline derivative, 2-ethyl-1-hexanol and decane discriminated asthma disease among patients with other atopic disorders. Results of this study indicate that refined metabolomic analysis of exhaled breath allows asthma with other coexisting atopic diseases discrimination in women of reproductive age.