bluuebunny/crossref_metadata_2025 huggingface dataset:
A Case Report and Literature Review of the Outcome of Linezolid-Induced Optic and Peripheral Neuropathy in Patients With Multidrug-Resistant Pulmonary TB
Authors: Bano Safia , Nawaz Ahmad , Numan Ahsan , Hassan Muhammad Aarish , Shafique Muhammad Bilal Ahmad
Published: June 2022

Linezolid is a second-line medication used to treat tuberculosis that has become resistant to multiple drugs. Linezolid has been shown to be effective in treating drug-resistant TB. However, long-term therapy is hampered by the related side effects, such as ocular and peripheral neuropathy. We recently encountered a 32-year-old male undergoing linezolid therapy for 12 months for multidrug-resistant tuberculosis who presented with progressive painless visual impairment and peripheral neuropathy symptoms in lower limbs as well as ataxic gait. Nerve conduction study findings of length-dependent axonal sensory polyneuropathy with bilateral optic neuropathy evident on fundoscopy suggested a case of toxic neuropathy. Following the termination of linezolid, follow-up visits revealed an improvement in visual symptoms. While there has been no discernible improvement or deterioration of peripheral neuropathy. In a developing country like Pakistan, where the rising number of cases of multidrug-resistant tuberculosis and its management is a major problem, physicians should be made aware of linezolid induced neuropathy so that close follow-up sessions for patients on long-term linezolid therapy can be arranged to avoid serious neurological consequences.
How to Test the Association Between Baseline Performance Level and the Modulatory Effects of Non-Invasive Brain Stimulation Techniques
Authors: Lega Carlotta , Cattaneo Luigi , Costantini Giulio
Published: June 2022

Behavioral effects of non-invasive brain stimulation techniques (NIBS) can dramatically change as a function of different factors (e.g., stimulation intensity, timing of stimulation). In this framework, lately there has been a growing interest toward the importance of considering the inter-individual differences in baseline performance and how they are related with behavioral NIBS effects. However, assessing how baseline performance level is associated with behavioral effects of brain stimulation techniques raises up crucial methodological issues. How can we test whether the performance at baseline is predictive of the effects of NIBS, when NIBS effects themselves are estimated with reference to baseline performance? In this perspective article, we discuss the limitations connected to widely used strategies for the analysis of the association between baseline value and NIBS effects, and review solutions to properly address this type of question.
NoLiTiA: An Open-Source Toolbox for Non-linear Time Series Analysis
Authors: Weber Immo , Oehrn Carina R.
Published: June 2022

In many scientific fields including neuroscience, climatology or physics, complex relationships can be described most parsimoniously by non-linear mechanics. Despite their relevance, many neuroscientists still apply linear estimates in order to evaluate complex interactions. This is partially due to the lack of a comprehensive compilation of non-linear methods. Available packages mostly specialize in only one aspect of non-linear time-series analysis and most often require some coding proficiency to use. Here, we introduce NoLiTiA, a free open-source MATLAB toolbox for non-linear time series analysis. In comparison to other currently available non-linear packages, NoLiTiA offers (1) an implementation of a broad range of classic and recently developed methods, (2) an implementation of newly proposed spatially and time-resolved recurrence amplitude analysis and (3) an intuitive environment accessible even to users with little coding experience due to a graphical user interface and batch-editor. The core methodology derives from three distinct fields of complex systems theory, including dynamical systems theory, recurrence quantification analysis and information theory. Besides established methodology including estimation of dynamic invariants like Lyapunov exponents and entropy-based measures, such as active information storage, we include recent developments of quantifying time-resolved aperiodic oscillations. In general, the toolbox will make non-linear methods accessible to the broad neuroscientific community engaged in time series processing.
Physicochemical, Sensory and Digestive Properties of Eel Burgers at Different Baking Temperatures
Authors: Li Jieyu , Shi Linfan , Ren Zhongyang , Weng Wuyin
Published: June 2022

The effect of baking temperature on the physicochemical, sensory and digestive properties of eel burgers was investigated. The moisture content of eel burgers gradually decreased with increased baking temperature, whereas the water-holding capacity remained unchanged. The breaking force of eel burgers baked at 160°C was significantly higher than that at other baking temperatures. With increased baking temperature from 100 to 220°C, amide I in the Fourier transform infrared spectroscopy of eel burgers shifted from 1,645 to 1,633 cm−1, and the peak intensity of 1,744 cm−1 initially increased and then decreased. When the baking temperature exceeded 160°C, the band intensity of protein aggregate increased gradually with increased baking temperature. Scanning electron microscopy result indicated that the muscle fibers in eel burgers contracted significantly with increased baking temperature, and a honeycomb-like network structure appeared in eel burgers baked at 220°C. The sulfur compounds in the eel burgers baked at 130°C were lower than those of the sample baked at 100°C, but it increased gradually with further increased baking temperature. The aftertaste astringency, richness, saltiness, and overall acceptability of eel burgers increased with increased baking temperature. The eel burgers baked at 130–160°C could be easily digested according to the in vitro digestibility and confocal laser confocal microscopy of gastrointestinal digests. In conclusion, the texture properties, barbecue aroma, and digestibility of eel burgers could be controlled by the baking temperature.
Identification of Novel Tumor Antigens and the Immune Landscapes of Bladder Cancer Patients for mRNA Vaccine Development
Authors: Wang Guixin , Gao Yukui , Chen Yanzhuo , Wang Keruo , Zhang Shicheng , Li Gang
Published: June 2022

Background

mRNA vaccines are a novel technology that provide a potential strategy for cancer treatment. However, few studies exist that are focused on the application and development of mRNA vaccines in bladder cancer (BLCA). Therefore, this study filtered candidate antigens and specific mRNA-suitable populations in BLCA via comprehensive multi-omics analysis.

Methods

Clinical information, follow-up information, and gene expression profiles were obtained from the TCGA and GEO databases. Somatic mutation and DNA copy number variation of BLCA were visualized by cBioPortal. Significant survival genes were analyzed by GEPIA2. TIMER was used to evaluate the connection between candidate antigens and infiltration of antigen-presenting cells. Consensus clustering analysis was performed to identify immune subtypes using the ConsensusClusterPlus package. The Monocle package was used to visualize the immune landscapes of each BLCA patient. Weighted gene co-expression network analysis (WGCNA) was used to identify key genes for mRNA vaccines.

Results

AP2S1, P3H4, and RAC3 were identified as candidate tumor-specific antigens for BLCA. Three immune subtypes were classified based on immune-related gene expression profiles. Patients with the BCS2 subtype were characterized as immune “cold” and exhibited upregulation of immunogenic cell death modulators, whereas patients with BCS1 and BCS3 were immune “hot” and had upregulation of immune checkpoints. Interestingly, patients with the BCS2 subtype had a better prognosis than other subtypes. The immune landscapes of each patient were visualized and revealed the heterogeneity within the BCS1 subtype. Finally, 13 key immune genes were identified.

Conclusions

AP2S1, P3H4, and RAC3 were identified as candidate tumor-specific antigens, and patients with the BCS2 and BCS1A subtypes were identified as candidate populations for mRNA vaccines. In summary, this study provides novel insights and a theoretical basis for mRNA vaccine development in BLCA and other malignancies.
Momordica charantia Exosome-Like Nanoparticles Exert Neuroprotective Effects Against Ischemic Brain Injury via Inhibiting Matrix Metalloproteinase 9 and Activating the AKT/GSK3β Signaling Pathway
Authors: Cai Heng , Huang Lin-Yan , Hong Rui , Song Jin-Xiu , Guo Xin-Jian , Zhou Wei , Hu Zhao-Li , Wang Wan , Wang Yan-Ling , Shen Jian-Gang , Qi Su-Hua
Published: June 2022

Plant exosome-like nanoparticles (ELNs) have shown great potential in treating tumor and inflammatory diseases, but the neuroprotective effect of plant ELNs remains unknown. In the present study, we isolated and characterized novel ELNs from Momordica charantia (MC) and investigated their neuroprotective effects against cerebral ischemia-reperfusion injury. In the present study, MC-ELNs were isolated by ultracentrifugation and characterized. Male Sprague–Dawley rats were subjected to middle cerebral artery occlusion (MCAO) and MC-ELN injection intravenously. The integrity of the blood–brain barrier (BBB) was examined by Evans blue staining and with the expression of matrix metalloproteinase 9 (MMP-9), claudin-5, and ZO-1. Neuronal apoptosis was evaluated by TUNEL and the expression of apoptotic proteins including Bcl2, Bax, and cleaved caspase 3. The major discoveries include: 1) Dil-labeled MC-ELNs were identified in the infarct area; 2) MC-ELN treatment significantly ameliorated BBB disruption, decreased infarct sizes, and reduced neurological deficit scores; 3) MC-ELN treatment obviously downregulated the expression of MMP-9 and upregulated the expression of ZO-1 and claudin-5. Small RNA-sequencing revealed that MC-ELN-derived miRNA5266 reduced MMP-9 expression. Furthermore, MC-ELN treatment significantly upregulated the AKT/GSK3β signaling pathway and attenuated neuronal apoptosis in HT22 cells. Taken together, these findings indicate that MC-ELNs attenuate ischemia-reperfusion–induced damage to the BBB and inhibit neuronal apoptosis probably via the upregulation of the AKT/GSK3β signaling pathway.
Stability of Woven Frames
Authors: Li Xue-Bin , Zhu Yu-Can
Published: June 2022

This article studies the stability of woven frames by introducing some special limits. We show that there exist certain relations among the different types of convergence of frames and obtain some new and more general stability results. As an application of these results, we provide a method for constructing woven frames.
Quantum Systems for Enhanced High Energy Particle Physics Detectors
Authors: Doser M. , Auffray E. , Brunbauer F.M. , Frank I. , Hillemanns H. , Orlandini G. , Kornakov G.
Published: June 2022

Developments in quantum technologies in the last decades have led to a wide range of applications, but have also resulted in numerous novel approaches to explore the low energy particle physics parameter space. The potential for applications of quantum technologies to high energy particle physics endeavors has however not yet been investigated to the same extent. In this paper, we propose a number of areas where specific approaches built on quantum systems such as low-dimensional systems (quantum dots, 2D atomic layers) or manipulations of ensembles of quantum systems (single atom or polyatomic systems in detectors or on detector surfaces) might lead to improved high energy particle physics detectors, specifically in the areas of calorimetry, tracking or timing.
Quantitative Compression Elastography With an Uncalibrated Stress Sensor
Authors: Rippy Justin R. , Singh Manmohan , Nair Achuth , Aglyamov Salavat , Larin Kirill V.
Published: June 2022

Tissue stiffness is a key biomechanical property that can be exploited for diagnostic and therapeutic purposes. Tissue stiffness is typically measured quantitatively via shear wave elastography or qualitatively through compressive strain elastography. This work focuses on merging the two by implementing an uncalibrated stress sensor to allow for the calculation of Young’s modulus during compression elastography. Our results show that quantitative compression elastography is able to measure Young’s modulus values in gelatin and tissue samples that agree well with uniaxial compression testing.
Calibration Methods of Atmospheric Aerosol Lidar and a Case Study of Haze Process
Authors: Chen Chao , Song Xiaoquan , Wang Zhangjun , Chen Yubao , Wang Xiaopeng , Bu Zhichao , Zhang Xi , Zhuang Quanfeng , Pan Xin , Li Hui , Zhang Feng , Wang Xiufen , Li Xianxin , Zheng Ronger
Published: June 2022

Lidar is a reliable tool for active remote sensing detection of atmospheric aerosols. A multi-wavelength aerosol lidar (MWAL) with 355 nm, 532 and 1064 nm as detection light sources has been developed and deployed for operational observations at Haidian District Meteorological Service of Beijing. The structure design, specifications, observation campaign, and detection principle of the MWAL are introduced. To ensure the accuracy and reliability of the lidar observation data, the calibration contents, and methods of lidar are proposed, including the correction, and gluing of the original data, the collimation of the transmitting and receiving optical axes, the testing of signal saturation, the correction of molecular Rayleigh fitting and the determination of the depolarization ratio correction factor. Finally, a haze process from 29 September to 2 October 2019 was observed and analyzed using the data of lidar, digital radiosonde, air quality and relative humidity observed by the Haidian District Meteorological Service. The detection results show the reliability of lidar which can effectively obtain the temporal and spatial variation characteristics of the haze. The profiles of aerosol extinction coefficient, potential temperature and relative humidity can be effectively used to analyze the haze thickness and the influence of relative humidity on aerosol particles. The data of air quality monitor shows that PM10 is the main pollutant and the ratio of PM2.5/PM10 is negatively correlated with relative humidity. Finally, the HYSPLIT trajectory tracking model of the National Oceanic and Atmospheric Administration (NOAA) is used to further study the source of pollutants in this haze process.
Cloning and Bioinformatics Analysis of GhArfGAP in Cotton (Gossypium hirsutum) Boll Abscission Layer With Ethylene Treatment
Authors: Chen Long , Liu AnFeng , Guo ZiWen , Jiang Hui , Luo Ling , Gao JunShan , Li DaHui , Ye SiHong , Guo Ning
Published: June 2022

With the continuous growth of the human population, the demand for fiber is also rising sharply. As one of the main fiber plants available globally, cotton fiber yield (Gossypium hirsutum) is affected by boll abscission, which is related to the formation of the abscission layer. Therefore, we explored the formation of the abscission layer in cotton. The formation of the abscission layer in the cotton boll stalk was promoted by exogenous ethylene. It was found that both the number of the Golgi apparatus and the number of stacking layers increased in the dissociated cells. The GhArfGAP gene family in cotton was screened by the bioinformatics method, and the species and evolutionary relationship of the GhArfGAP gene family were analyzed. qRT-PCR showed that GhArfGAP13, GhArfGAP15, GhArfGAP25, and GhArfGAP34 in cotton had spatiotemporal-specific expression patterns. Subcellular localization suggested that GhArfGAP25 played a role in the Golgi apparatus. The expression of GhArfGAP25 in transgenic Arabidopsis thaliana is increased in the roots, stems, and leaves. Finally, we found that ethylene could induce the formation of the abscission layer in cotton. GhArfGAP13, GhArfGAP15, GhArfGAP25, and GhArfGAP34 might regulate the changes in the Golgi apparatus in the abscission layer. Taken together, the findings provide new ideas for the study of the formation of cotton abscission.
Disentangling the Role of Deviant Letter Position on Cognate Word Processing
Authors: Comesaña Montserrat , Haro Juan , Macizo Pedro , Ferré Pilar
Published: June 2022

The way of coding letter position has been extensively assessed during the recognition of native words, leading to the development of a new generation of models that assume more flexible letter position coding schemes compared to classical computational models such as the interactive activation (IA) model. However, determining whether similar letter position encoding mechanisms occur during the bilingual word recognition has been largely less explored despite its implications for the leading model of bilingual word recognition (multilink) as it assumes the input-coding scheme of the IA model. In this study, we aimed to examine this issue through the manipulation of the position of the deviant letter of cognate words (external and internal letters). Two experiments were conducted with Catalan–Spanish bilinguals (a masked priming lexical decision task and a two-alternative forced-choice task) and their respective monolingual controls. The results revealed a differential processing for the first letter in comparison to the other letters as well as modulations as a function of language cue, suggesting amendments to the input-coding scheme of the multilink model.
Teaching in Uncertain Times: Expanding the Scope of Extraneous Cognitive Load in the Cognitive Load Theory
Authors: Taylor Tracey A. H. , Kamel-ElSayed Suzan , Grogan James F. , Hajj Hussein Inaya , Lerchenfeldt Sarah , Mohiyeddini Changiz
Published: June 2022

The COVID-19 pandemic caused an unprecedented and highly threatening, constrained, and confusing social and educational environment, we decided to expand the traditional focus of the extraneous load in Cognitive Load Theory (CLT) acknowledging the psychological environment in which learning occurs. We therefore adapted and implemented principles of the CLT to reduce extraneous load for our students by facilitating their educational activities. Given previous empirical support for the principles of CLT, it was expected that the adoption of these principles might enable our students to cultivate attitudes and skills across multiple domains such as online learning and presentation technologies, implementing and maintaining a “classroom atmosphere” in a virtual environment, participating in discussions among large online groups of students, facilitating group work, providing virtual office hours for students, and proactively planning for upcoming semesters.
The Impact of New Entrepreneurial Spirit on Cultivating Entrepreneurial Values and Entrepreneurial Ability of College Students
Authors: Li Ping , Chen Xiaozhou
Published: June 2022

The objectives were to deeply study the impact of new entrepreneurial spirit on the cultivation of entrepreneurial values and entrepreneurial ability of college students. First, the influencing factors of college students' entrepreneurial values were analyzed based on new media, entrepreneurial spirit, entrepreneurial values, and other related theories. Second, the corresponding questionnaire was designed and elaborated on the four aspects of college students' entrepreneurial values, namely, entrepreneurial competence, entrepreneurial risk, and entrepreneurial ethics. Finally, the data results of the questionnaire were studied. The results show that in entrepreneurial values of college students, they believe that entrepreneurship is the most important for personal development, with an overall average of 3.86. In the point of view of entrepreneurial competence, they think that independent learning ability plays a significant role in entrepreneurship, with an average of 3.91. In the view of entrepreneurial ethics, they consider that the law is the most crucial during the entrepreneurial, with an average value of 4.35. It means that college students still have certain legal knowledge. After analyzing the results of the questionnaire on entrepreneurial factors, it is found that college students lack social experience and have a low ability to take risks. Meanwhile, entertainment is the first choice for college students on new media platforms, and the time of viewing is more than 5 h/day. Therefore, the influence of new entrepreneurial spirit is researched on the cultivation of entrepreneurial values and entrepreneurial ability of college students, which provides a new theoretical basis and optimization direction for entrepreneurial education in universities in the future.
Predicting Pro-environmental Intention and Behavior Based on Justice Sensitivity, Moral Disengagement, and Moral Emotions – Results of Two Quota-Sampling Surveys
Authors: Nicolai Susanne , Franikowski Philipp , Stoll-Kleemann Susanne
Published: June 2022

The effects of climate change lead to increasing social injustice and hence justice is intrinsically linked to a socio-ecological transformation. In this study, we investigate whether justice sensitivity motivates pro-environmental intention (PEI) and behavior (PEB) and, if so, to what extent emotions and moral disengagement determine this process. For this purpose, we conducted two quota-sampling surveys (Study 1: N = 174, Study 2: N = 880). Multiple regression analyses in both studies suggest that a higher perception of injustice from a perpetrator’s, beneficiary’s, and observer’s perspective is associated with an increased PEI. However, moral disengagement best predicted PEB and PEI. Guilt and authentic pride were found to be emotional predictors of PEI. Additionally, mediation analyses demonstrated that guilt mediates the connection between both perpetrator and beneficiary sensitivity and PEI. These results suggest that when the predominant originators of climate change (i.e., individuals from industrialized countries) perceive global climate injustice from the perspective of a beneficiary or a perpetrator, they experience guilt and have a higher PEI. Based on this mechanism, it seems promising to render global injustice more salient to those responsible for activities that lead to climate change to motivate them to adapt their behavior. The role of moral disengagement and victim sensitivity as barriers to pro-environmental behavior is discussed in this context.
Individual vs. Team Sport Failure—Similarities, Differences, and Current Developments
Authors: Wergin V. Vanessa , Mallett Clifford J. , Beckmann Jürgen
Published: June 2022

The construct of “choking under pressure” is concerned with the phenomenon of unexpected, sudden, and significant declines in individual athletes’ performances in important situations and has received empirical attention in the field of sport psychology. Although a number of theories about the reasons for the occurrence of choking under pressure exist and several intervention approaches have been developed, underlying mechanisms of choking are still under debate and the effectiveness of existing interventions remains contested. These sudden performance declines also occur in team sport. “Collective sport team collapse,” which describes the situation when an entire sport team underperforms significantly within an important competitive situation, has received less empirical attention, in comparison to individual choking research. While there are a few studies that have investigated causes of collective team collapse, understandably, there has been limited empirical investigation of preventative and intervention strategies. Although the two constructs appear to share several similar characteristics and mechanisms, research has not yet examined the conceptual, theoretical, empirical, and practical links between choking under pressure and collective sport team collapse. In this review article, we seek to examine these similarities and differences and identify new ways of thinking about future interventions. Furthermore, current empirical understandings in the field of choking under pressure and collective sport team collapse are presented and the most effective intervention approaches for both constructs are introduced. On the basis of this examination, we modestly make some initial recommendations for sport psychological practitioners and future research.
Seeing Through the Façade of Anorexia: A Grounded Theory of Emotional Change Processes Associated With Recovery From Anorexia Nervosa
Authors: Drinkwater Danielle , Holttum Sue , Lavender Tony , Startup Helen , Oldershaw Anna
Published: June 2022

Objectives

Difficulties in managing emotions have been implicated in the development and maintenance of anorexia nervosa (AN), and psychological treatment models seek to address this in putative targets of change. Yet the field of psychotherapy remains unclear and insufficiently evidenced about the process of change and how this is actually achieved, including in what steps and in what order within clinical treatment. This qualitative study sought to develop theory about the process of emotional change during recovery from anorexia.

Methods

Semi-structured interviews were carried out with nine women currently engaged in psychological treatment for anorexia. Interviews included questions pertaining to participants’ experience of anorexia, emotions, and emotion management. A constructivist version of grounded theory was employed.

Results

The analysis produced 10 major categories, comprising over 60 focused codes. Categories were clustered together into three super categories, reflecting 3 distinct but interrelated phases of participants’ journeys toward recovery. The phases were: (1) Coping in a world of uncertainty, (2) Seeing through the façade of anorexia, and (3) Recovery and growth. Whilst movement toward later positions often appeared to be contingent on earlier ones, the analysis suggests that this was not an entirely linear process and that participants moved between positions as they grappled with the process of change. Participants came to view behaviors associated with anorexia as emotion-management strategies that were not working and as a façade. As they moved toward recovery and growth, they became less confined by their need for safety, and to see emotions as meaningful and valuable. Becoming more connected to emotional experience and expression, coincided with positive shifts in their intra and interpersonal relationships.

Conclusion

These findings support the recent shift toward emotion-focused models of anorexia. They also highlight an important focus in supporting individuals with AN to connect with, and be guided by, emotional experiences in their relationships with themselves and the world around them. This new grounded theory offers a putative process of change that could be utilized to guide intervention development.
The Effectiveness of Self-Guided Digital Interventions to Improve Physical Activity and Exercise Outcomes for People With Chronic Conditions: A Systematic Review and Meta-Analysis
Authors: Stavric Verna , Kayes Nicola M. , Rashid Usman , Saywell Nicola L.
Published: June 2022

Objective

The aim of this systematic review was to determine the effectiveness of self-guided digital physical activity (PA) and exercise interventions to improve physical activity and exercise (PA&E) outcomes for people living with chronic health conditions. Digital health interventions, especially those with minimal human contact, may offer a sustainable solution to accessing ongoing services and support for this population.

Methods

A comprehensive and systematic search was conducted up to December 2021, through seven databases, for randomized trials that evaluated the effect of self-guided web- or internet-based PA interventions on physical activity or exercise outcomes. Included studies had to have interventions with minimal human contact and interaction with participants needed to be automatically generated. All studies were screened for eligibility and relevant data were extracted. Two independent reviewers assessed the risk of bias using the Cochrane risk of bias tool. Standardized mean differences and 95% confidence intervals (CI) were calculated. PA data were pooled, and forest plots were generated.

Results

Sixteen studies met the eligibility criteria and included a total of 2,439 participants. There was wide variation in health conditions and intervention characteristics in mode and parameters of delivery, and in the application of theory and behavioral strategies. Self-reported PA in the intervention group was greater than controls at the end of the intervention [standardized mean difference (SMD) 0.2, 95% CI = 0.1, 0.3] and at follow up (SMD 0.3, 95% CI 0.2–0.5). The difference in objectively measured PA was small and non-significant (SMD 0.3, 95% CI −0.2 to 0.9). All interventions included behavioral strategies and ten of the sixteen were underpinned by theory.

Conclusions

Self-guided digital PA&E interventions provided a positive effect on PA immediately after the intervention. An unexpected and positive finding was a sustained increase in PA at follow-up, particularly for interventions where the behavioral strategies were underpinned by a theoretical framework. Interventions with minimal contact have the potential to support sustained PA engagement at least as well as interventions with supervision.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD42019132464.
Effect of Two Different Drug-Resistant Staphylococcus aureus Strains on the Physiological Properties of MAC-T Cells and Their Transcriptome Analysis
Authors: Yan Lijiao , Yang Yuze , Ma Xiaojun , Wei Lianhua , Wan Xuerui , Zhang Zhao , Ding Jucai , Peng Jie , Liu Guo , Gou Huitian , Wang Chuan , Zhang Xiaoli
Published: June 2022

Staphylococcus aureus (S. aureus) is one of the main pathogens causing mastitis in dairy cows. The current work mainly focuses on the pathway of apoptosis induction in MAC-T cells caused by S. aureus infection or other factors. However, the physiological characteristics of S. aureus infected MAC-T cells and the resulting mRNA expression profile remain unknown particularly in the case of diverse drug resistant strains. Methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains were used to infect MAC-T cells to investigate this issue. The adhesion, invasion and apoptosis ability of MRSA-infected group and MSSA-infected group was assessed over time (2, 4, 6, 8, and 12 h). After 8 h, the RNA sequencing was conducted on the MRSA-infected and the MSSA-infected with uninfected MAC-T cells as controls. The results showed that the adhesion and invasion ability of MRSA-infected and MSSA-infected to MAC-T cells increased and then decreased with infection time, peaking at 8 h. The adhesion and invasion rates of the MSSA-infected were substantially lower than those of the MRSA-infected, and the invasion rate of the MSSA-infected group was nearly non-existent. Then the apoptosis rate of MAC-T cells increased as the infection time increased. The transcriptome analysis revealed 549 differentially expressed mRNAs and 390 differentially expressed mRNAs in MRSA-infected and MSSA-infected MAC-T cells, respectively, compared to the uninfected MAC-T cells. According to GO analysis, these differentially expressed genes were involved in immune response, inflammation, apoptosis, and other processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated the following pathways were linked to adhesion, invasion inflammation and apoptosis, including AMPK, FOXO, HIF-1, IL-17, JAK-STAT, MAPK, mTOR, NF-κB, p53, PI3K-Akt, TNF, Toll-like receptor, Rap1, RAS, prion disease, the bacterial invasion of epithelial cells pathway. We found 86 DEGs from 41 KEGG-enriched pathways associated with adhesion, invasion, apoptosis, and inflammation, all of which were implicated in MAC-T cells resistance to MRSA and MSSA infection. This study offers helpful data toward understanding the effect of different drug-resistant S. aureus on dairy cow mammary epithelial cells and aid in the prevention of mastitis in the dairy industry.
Effects of Galactomannan Oligosaccharides on Growth Performance, Mycotoxin Detoxification, Serum Biochemistry, and Hematology of Goats Fed Mycotoxins-Contaminated Diets
Authors: Wu Yicheng , Ren Ao , Lv Xiaokang , Ran Tao , Zhang Guijie , Zhou Chuanshe , Tan Zhiliang
Published: June 2022

This study was conducted to investigate the protective effects of mycotoxin adsorbent galactomannan oligosaccharides (GMOS) on growth performance, fermentation parameters, mycotoxins residues, serum biochemistry and oxidative stress parameters of the goats. The in vitro test indicated that 0.05% GMOS outperformed yeast cell wall (YCW) and montmorillonite (MMT) in aflatoxins absorption. Then 20 3-month-old Xiangdong black goats (15.0 ± 1.9 kg) were randomly divided into two dietary treatments for the animal test. The control group (CON group) was fed a multi-mycotoxins contaminated diet, whereas the experimental group (GMOS group) received multi-mycotoxins contaminated diet plus 0.05% GMOS. The trail lasted for 60 days, with 12 days of adaptation period and 48 days of formal experiment period. There were no treatment effects (P > 0.10) on growth performance, serum antioxidant capacity and activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). The concentrations of zearalenone in the rumen were lower (P < 0.05) in the GMOS group. GMOS significantly reduced (P < 0.05) propionate concentration in the cecum, resulting in a rise (P < 0.01) in acetate/propionate ratio in GMOS as compared to CON. Goats of GMOS exhibited considerably greater (P < 0.05) levels of creatine kinase but lower (P = 0.02) levels of creatinine than CON. Compared with CON, GMOS supplementation significantly increased (P < 0.05) platelet count (PLT), platelet volume distribution width (PDW), and platelet hematocrit (PCT), while decreased (P < 0.05) albumin content (ALB). The 0.05% GMOS protected goats in ruminal fermentation parameters, mycotoxins residues and serum biochemistry. Moreover, GMOS had no adverse effect on goat health. To our knowledge, this is the first report of GMOS in small ruminants. These findings suggested the feasibility of dietary GMOS as a health-maintaining addictive in goat diets.
A Comparative Field Evaluation of the Effect of Growth Performance Between Porcine Circovirus Type 2a (PCV2a)- and PCV2b-Based Bivalent Vaccines Containing PCV2 and Mycoplasma hyopneumoniae
Authors: Cho Hyejean , Oh Taehwan , Suh Jeongmin , Chae Chanhee
Published: June 2022

The objective of this study was to compare two different bivalent vaccines containing porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae. One vaccine contained PCV2a and the other contained PCV2b, and both were administered on a farm suffering from subclinical PCV2d infection and enzootic pneumonia. A total of 180 pigs were randomly divided into 3 groups (60 pigs per group; male pigs = 30 and female pigs = 30). Bivalent vaccination significantly improved growth performance in both vaccinated groups as compared to the unvaccinated (UnVac) group. Growth performance measured by body weight and average daily weight gain (ADWG) was not significantly different between the two bivalent-vaccinated groups (VacA and VacB). Both bivalent vaccines elicited high levels of neutralizing antibodies and interferon-γ secreting cells (IFN-γ-SC) against PCV2d, leading to a reduction in the levels of PCV2d blood viral load as compared to unvaccinated animals. Similarly, both bivalent vaccines elicited high levels of IFN-γ-SC against M. hyopneumoniae that reduced the level of M. hyopneumoniae laryngeal viral loads as compared to unvaccinated animals. Significant differences in severity of lung and lymphoid lesions were observed in both vaccinated groups as compared to the UnVac group. These comparative field data demonstrated that both bivalent vaccines are good candidates for controlling subclinical PCV2d infection and enzootic pneumonia in swine farms suffering from an existing infection.
Biopsy Characteristics, Subtypes, and Prognostic Features in 107 Cases of Feline Presumed Immune-Mediated Polyneuropathy
Authors: Kolb Ninja , Matiasek Kaspar , van Renen Jana , Fischer Andrea , Zablotski Yury , Wieländer Franziska , Nessler Jasmin , Tipold Andrea , Cappello Rodolfo , Flegel Thomas , Loderstedt Shenja , Dietzel Josephine , Gnirs Kirsten , Rentmeister Kai , Rupp Stephan , von Klopmann Thilo , Steffen Frank , Jurina Konrad , Del Vecchio Omar V. , Deutschland Martin , König Florian , Gandini Gualtiero , Harcourt-Brown Tom , Kornberg Marion , Bianchi Ezio , Gagliardo Teresa , Menchetti Marika , Schenk Henning , Tabanez Joana , Rosati Marco
Published: June 2022

Inflammatory polyradiculoneuropathy (IMPN) is one of the causes of sudden onset of neuromuscular signs such as para-/tetraparesis in young cats. Even though most cases have a favorable outcome, persistent deficits, relapses, and progressive courses are occasionally seen. As clinical presentation does not always appear to predict outcome and risk of recurrence, this study was initiated to screen for prognostic biopsy findings in a large cohort of histologically confirmed IMPN cases with clinical follow-up. In total, nerve and muscle specimens of 107 cats with biopsy diagnosis of presumed autoreactive inflammatory polyneuropathy and 22 control cases were reviewed by two blinded raters for a set of 36 histological parameters. To identify patterns and subtypes of IMPN, hierarchical k-means clustering of 33 histologic variables was performed. Then, the impact of histological parameters on IMPN outcome was evaluated via an univariate analysis to identify variables for the final multivariate model. The data on immediate outcome and follow-up were collected from submitting neurologists using a purpose-designed questionnaire. Hierarchical k-means clustering sorted the tissues into 4 main categories: cluster 1 (44/129) represents a purely inflammatory IMPN picture, whereas cluster 2 (47/129) was accompanied by demyelinating features and cluster 3 (16/129) by Wallerian degeneration. Cluster 4 (22/129) reflects normal tissues from non-neuropathic control cats. Returned questionnaires provided detailed information on outcome in 63 animals. They were categorized into recovered and non-recovered. Thereby, fiber-invasive infiltrates by mononuclear cells and mild fiber loss in intramuscular nerve branches correlated with higher probabilities of recovery. Remyelination in semithin sections, on the other hand, is correlated with a less favorable outcome. Animals grouping in cluster 1 had a tendency to a higher probability of recovery compared to other clusters. In conclusion, diagnosis of feline IMPN from nerve and muscle biopsies allowed for the identification of histologic features that were positively or negatively correlated with outcome.
Case Report: The First Report on Moraxella canis Isolation From Corneal Ulcer in a Bulldog
Authors: Wang Zhihao , Guo Long , Li Jun , Li Jianji , Cui Luying , Dong Junsheng , Wang Heng
Published: June 2022

A 5-year-old castrated male bulldog was diagnosed with a corneal ulcer accompanied by edema and conjunctival hyperemia. Ophthalmic examination and microbiological analysis were performed, and the bacteria were found to be gram-negative and globular. The isolated clone was identified as Moraxella canis (MZ579539) via MALDI-TOF MS and 16S rDNA sequencing. Antimicrobial susceptibility testing showed that the bacteria were sensitive to tetracycline and chloramphenicol, but resistant to levofloxacin and ciprofloxacin. After a conjunctival flap was placed, tobramycin ophthalmic solution and 5% sodium hyaluronate were administered. Following surgery, the ulcer was effectively controlled, and after 3 weeks, the cornea healed. This is the first case report of a canine corneal ulcer associated with M. canis, which should be considered when corneal ulceration or keratitis were suspected.
Electromechanical Performance of Biocompatible Piezoelectric Thin-Films
Authors: Mishra S. Ranjan , Hassani Fard Soran , Sheikh Taha , Behdinan Kamran
Published: June 2022

The present study analyzed a computational model to evaluate the electromechanical properties of the AlN, BaTiO3, ZnO, PVDF, and KNN-NTK thin-films. With the rise in sustainable energy options for health monitoring devices and smart wearable sensors, developers need a scale to compare the popular biocompatible piezoelectric materials. Cantilever-based energy harvesting technologies are seldom used in sophisticated and efficient biosensors. Such approaches only study transverse sensor loading and are confined to fewer excitation models than real-world applications. The present research analyses transverse vibratory and axial-loading responses to help design such sensors. A thin-film strip (50 × 20 × 0.1 mm) of each sample was examined under volumetric body load stimulation and time-based axial displacement in both the d31 and d33 piezoelectric energy generation modes. By collecting evidence from the literature of the material performance, properties, and performing a validated finite element study to evaluate these performances, the study compared them with lead-based non-biocompatible materials such as PZT and PMN-PT under comparable boundary conditions. Based on the present study, biocompatible materials are swiftly catching up to their predecessors. However, there is still a significant voltage and power output performance disparity that may be difficult to close based on the method of excitation (i.e., transverse, axial, or shear. According to this study, BaTiO3 and PVDF are recommended for cantilever-based energy harvester setups and axially-loaded configurations.
Position Accuracy Assessment of a UAV-Mounted Sequoia+ Multispectral Camera Using a Robotic Total Station
Authors: Paraforos Dimitrios S. , Sharipov Galibjon M. , Heiß Andreas , Griepentrog Hans W.
Published: June 2022

Remote sensing data in agriculture that are originating from unmanned aerial vehicles (UAV)-mounted multispectral cameras offer substantial information in assessing crop status, as well as in developing prescription maps for site-specific variable rate applications. The position accuracy of the multispectral imagery plays an important role in the quality of the final prescription maps and how well the latter correspond to the specific spatial characteristics. Although software products and developed algorithms are important in offering position corrections, they are time- and cost-intensive. The paper presents a methodology to assess the accuracy of the imagery obtained by using a mounted target prism on the UAV, which is tracked by a ground-based total station. A Parrot Sequoia+ multispectral camera was used that is widely utilized in agriculture-related remote sensing applications. Two sets of experiments were performed following routes that go along the north–south and east–west axes, while the cross-track error was calculated for all three planes, but also three-dimensional (3D) space. From the results, it was indicated that the camera’s D-GNSS receiver can offer imagery with a 3D position accuracy of up to 3.79 m, while the accuracy in the horizontal plane is higher compared to the vertical ones.
Anastomosis between Median and Musculocutaneous Nerve: Presentation of a Very Rare Anatomical Variation in Comparison to Classical Divisions
Authors: Barone Rosario , D’Amico Agata Grazia , Di Lorenzo Noemi , Di Grado Grazia Laura , Matranga Egle , Spinoso Giulio , Bavuso Leonardo Luca , Marino Gammazza Antonella , Rappa Francesca , Bucchieri Fabio , Cappello Francesco , Piotrowska Weronika , Spodnik Jan Henryk , Spodnik Edyta , Wójcik Sławomir
Published: June 2022

The musculocutaneous nerve (MCN) is the terminal branch of the lateral cord of the brachial plexus, and emerges at the inferior border of pectoralis minor muscle. The nerve can interact with the median nerve (MN), adhering to the nerve and sharing fibers with it. During anatomical dissection of twelve cadavers, we have detected a rare variation of the anastomosis between MCN and MN. The knowledge of this anatomical variation could be of great relevance during surgical and clinical practices.
Risk Factors and Clinical Presentation in Dogs with Increased Serum Pancreatic Lipase Concentrations—A Descriptive Analysis
Authors: Cridge Harry , Scott Nicole , Steiner Jörg M.
Published: June 2022

Limited data exist regarding the full array of clinical signs seen in dogs with pancreatitis and potential risk factors for the disease. Laboratory submissions from the Gastrointestinal Laboratory at Texas A&M University were retrospectively reviewed for dogs with an increased serum pancreatic lipase immunoreactivity (cPLI) concentration (≥400 µg/L), and an internet-based survey was distributed to the attending veterinarian and/or technician on each case. The survey contained questions related to (i) clinical signs, (ii) prior gastrointestinal upset, (iii) comorbidities, (iv) pre-existing medical therapies, and (v) dietary history. One hundred and seventy (170) survey responses were recorded. The top three clinical signs reported were inappetence (62%), diarrhea (53%), and vomiting (49%). Abdominal pain was noted in only 32% of dogs, likely associated with poor pain detection. Additionally, the majority of dogs (71%) had prior episodes of gastrointestinal upset within the past 12 months, lending support for the commonality of recurrent acute pancreatitis, or acute on chronic disease. Hepatobiliary abnormalities (24%) were the most common concurrent disease, and endocrine disorders were seen in a low proportion of respondents (5–8%). Adult maintenance diets (65%), dog treats (40%), and human foods (29%) were commonly consumed by dogs prior to the discovery of increased cPLI concentration.
Clinical Trial for Evaluating the Effectiveness and Safety of a New Dental Plaque Removal Device: Microscale Mist Unit
Authors: Hihara Hiroki , Izumita Kuniyuki , Iwatsu Misato , Sato Tomoya , Tagaino Ryo , Shobara Kenta , Shinohara Yuta , Hatakeyama Takanori , Kayaba Chie , Sato Mariko , Tokue Ayako , Sugawara Tomoko , Ashino Kanamai , Ikeda Koji , Aida Jun , Sasaki Keiichi
Published: June 2022

This study evaluates the effectiveness and safety of a microscale mist unit (MSM-UNIT) that sprays high-speed fine water droplets to remove dental plaque adhering to the oral mucosa (tongue and palate) and tooth surface. Fifteen patients who had difficulty self-managing sufficient oral care were included in this study. Effectiveness was evaluated for at least five patients’ tongues, palate mucosas, and tooth surfaces, and safety evaluation was conducted at all three sites for all patients. Effectiveness was evaluated using the rate of degree of dental plaque removal. Safety was evaluated using a numerical rating scale (NRS) for pain and symptoms of inflammation. An operator who performed treatment and an evaluator who evaluated effectiveness and safety were designated. In addition, an image judgment committee judged effectiveness. Although evaluation of the tongue varied between the evaluators and the image judgment committee, the rates of degree for all plaque removal increased in all regions. In addition, low pain NRS results and minimal symptoms of inflammation were observed and within an acceptable range. The MSM-UNIT can be used effectively and safely for removing oral plaque not only from teeth, but also from the oral mucosa.