bluuebunny/crossref_metadata_2025 huggingface dataset:
Tracking e-reading behavior: uncovering the effects of task context, electronic experience, and motivation
Authors: Vuorinen Pauliina Tea Eleonoora , Tatler Benjamin W. , Keller Frank
Published: January 2024

Although electronic reading of fiction has become mainstream, little is known about how electronic texts are read, and whether this behavior is connected to readers’ motivation or experience reading electronically. To address this gap, sixty undergraduate students’ reading behavior was tracked while reading a 15-page short story. A novel method was used to study participants’ frequency of task-switching, their reading speed, and navigational patterns unobtrusively, outside of the lab. Reading behavior was analyzed by two multilevel models to assess (1) whether variance in behavior could be predicted by the task context, such as location in text or timing of reading sessions, and (2) whether behavior was connected to participants’ situational motivation to read the short story, their contextual motivation toward reading as an activity, or their task-relevant electronic experience. Our results showed that highly experienced and avid readers reacted to text difficulty more adaptively, indicating that motivation and electronic experience may have a key role in supporting comprehension. In contrast, situational motivation was not associated with reading behavior, contrary to our expectations. These findings provide a significant contribution to our understanding of e-reading, which can be used as a foundation to support recreational reading engagement on digital devices.
Adaptivity in the inclusive transition to school
Authors: Then Daniel , Pohlmann-Rother Sanna
Published: January 2024

The transition to school is a key juncture in an individual’s educational trajectory, with far-reaching effects on the development of children and their families. Successful transitions require flexibility in the design of the transition process, addressing the needs of the persons involved in anadaptivemanner. Adaptivity is also considered crucial for the success of inclusive transitions. However, a systematic breakdown of the aspects that characterize the concept of adaptivity in the context of inclusive school entry is not available at this point. This article therefore provides a conceptualization of adaptivity in the inclusive transition to school as well as a review of the current literature focusing this topic. The goal is to develop a model that structures the various aspects of adaptivity at school entry and offers an overview of the way these aspects are important to design the transition successfully according to current findings of empirical research. Building on a concept of transitions informed by ecological systems theory, we are guided by the assumption that adaptivity at transition to school may occur in three forms: as a feature of the persons involved in the transition; as a feature of the processes that moderate the course of the transition; and as a feature of the structures that frame the transition. Based on this distinction, we develop a model that presents adaptivity in the inclusive transition to school.
Transdisciplinary education for sustainability. Creativity and awareness in teacher training
Authors: Echegoyen-Sanz Yolanda , Morote Álvaro , Martín-Ezpeleta Antonio
Published: January 2024

Teacher training is a strategic aspect to achieve quality education and, therefore, bring closer Horizon 2030 and sustainability. The aim of this work is to present the outcome of an innovative transdisciplinary educational proposal with 176 primary teachers in training with the goal of raising their sustainability awareness. It was implemented at the same time in a Social sciences subject and in an Social sciences one demonstrating both its transdisciplinary nature and the fact that sustainability is a transversal aspect in the curriculum. A mixed methods research design was used. Work products were analyzed, such as the creation of a digital story for children about sustainability and some related activities. In addition, the perceived impact of the educational experience in the teaching-learning process and sustainability attitudes were assessed using two validated questionnaires. The results show a high level of perception of the impact of the experience in the motivation of preservice teachers, the learning of concepts related to the subject, and, especially, the usefulness of this proposal for their professional future. Furthermore, their sustainability attitudes at the end of the semester were very high, with scores above 4 (out of 5) for all measured dimensions. The relevance of advocating for a transdisciplinary education for sustainability, which begins in teacher training faculties, is concluded.
An audit of medullary thyroid carcinoma from a tertiary care hospital in northwest India
Authors: Chakraborty Ananda Mohan , Rai Ashutosh , Pal Rimesh , Mukherjee Soham , Dahiya Divya , Kumar Rajinder , Saikia Uma Nahar , Panda Naresh Kumar , Bhadada Sanjay Kumar , Dutta Pinaki
Published: January 2024

Introduction

Medullary thyroid carcinoma (MTC) is a rare thyroid malignancy originating from parafollicular C cells. It accounts for 5%–10% of all thyroid malignancies.

Methods

An ambispective analysis of pathologically proven MTC presented in a tertiary care hospital in northwest India was performed after considering demography, clinical manifestation, RET mutation status, management, and outcome as denominators.

Results

Among 2,735 thyroid malignancy cases who presented to our institute in the last 10 years (2012–2022), 78 (3%) had MTC with a mean age of presentation of 43 ± 11 years; 60% of them were female. The median duration of symptoms was 23 months (IQR 12–36 months). The most common presenting complaint was goiter with lymphadenopathy (80.8%). Among the atypical presentations, one each had ectopic Cushing’s syndrome, hypertensive crisis in pregnancy due to pheochromocytoma, synchronous chondrosarcoma, and Von Hippel–Lindau disease spectrum. Median calcitonin and carcinoembryonic antigen (CEA) levels at presentation were 1,274 pg/mL (n = 64) and 149 ng/mL (n = 39), respectively. Twenty-two patients were germline RET mutation-positive, and they presented at a younger age. Majority of the patients presented with stage IV disease. Surgery was the primary modality of therapy. Twenty-nine patients received radiotherapy and 25 patients received tyrosine kinase inhibitors (TKIs). Nine patients received peptide receptor radiotherapy (PRRT) with Lu-177 with neoadjuvant capecitabine. Median progression-free survival (PFS) was 60 months. Patients without structurally and biochemically residual disease and stable disease after the first modality of therapy (Log-rank 11.4; p = 0.004) had a better PFS. Female patients (Log-rank: 9.5; p = 0.002) had a better PFS than male patients.

Conclusion

This study showed that MTC comprises 3% of thyroid malignancies with a female preponderance. RET mutation-positive patients had a younger age at presentation. Surgery was the first-line therapy. Radiotherapy, TKI, and PRRT were given as a part of second-line or third-line therapy due to persistent disease and/or disease recurrence. The median PFS was better in female patients and in patients who had no residual lesions and stable disease after the primary modality of therapy.
Effects and safety of Ginkgo biloba on blood metabolism in type 2 diabetes mellitus: a systematic review and meta-analysis
Authors: Zou Huimin , Fang Jingxian , Han Yu , Hu Xue , Meng Jian , Huang Fang , Xu Hui , Lu Chengfei , Wang Yiwen , Zhang Lili , Dong Xiaohong , Yu Yanmei , Guo Yu , Gu Qing , Wang Suijun
Published: January 2024

Background

There has existed controversy regarding the use of Ginkgo biloba (GKB) for blood metabolism among type 2 diabetes mellitus(T2DM) patients, and we tried to analyze the effects and safety of GKB on T2DM patients.

Methods

We conducted a literature search between January 2003 and December 2022 of seven online databases (PubMed, Scopus, Embase, Google Scholar, Web of Sciences, Cochrane Library, and China National Knowledge Infrastructure). A systematic literature review and meta-analysis were performed to compare the effects and safety of GKB among T2DM patients. Four groups of parameters were extracted and analyzed: hemorheology parameters, lipid profile, glycemic control markers, and adverse events.

Results

In the end, 13 eligible articles with 11 indicators among 1573 patients were included. In the hemorheology parameters section, GKB showed significantly lower plasma viscosity (PV) (SMD=-0.91, 95%CI [-1.45, -0.36], P<0.01) and hematocrit (Hct) (SMD=-0.60, 95%CI [-0.97, -0.24], P<0.01) than the control group. GKB shoed higher velocity of the dorsalis pedis artery (VDPA) (SMD=0.51, 95%CI [0.26, 0.76], P<0.01) and ankle brachial index (ABI) (SMD=0.71, 95%CI [0.32, 1.10], P<0.01) than the control. In both the lipid profile and glycemic control markers sections, we did not find any difference between GKB and control groups, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), hemoglobin A1c (HbA1c), and fasting serum glucose (FSG). In addition, we saw no difference in adverse events (AE). The sensitivity analysis and funnel plot showed that the results in this research were robust and had no publication bias.

Conclusion

In conclusion, GKB might safely reduce the risk of peripheral arterial or even systemic cardiovascular disease. However, GKB did not directly improve lipid and blood glucose levels in T2DM patients.

Systematic review registration

https://inplasy.com/, identifier INPLASY202350096.
Safety and efficacy of autologous bone marrow clot as a multifunctional bioscaffold for instrumental posterior lumbar fusion: a 1-year follow-up pilot study
Authors: Salamanna Francesca , Tedesco Giuseppe , Sartori Maria , Griffoni Cristiana , Spinnato Paolo , Romeo Paolo , Ghermandi Riccardo , Fini Milena , Giavaresi Gianluca , Gasbarrini Alessandro , Barbanti Brodano Giovanni
Published: January 2024

Background

Bone marrow aspirate (BMA), when combined with graft substitutes, has long been introduced as a promising alternative to iliac crest bone graft in spinal fusion. However, the use of BMA is limited by the absence of a standardized procedure, a structural texture, and the potential for diffusion away from the implant site. Recently, the potential use of a new formulation of BMA, named BMA clot, has been preclinically described. In this report, we present the results of a prospective pilot clinical study aimed at evaluating the safety and efficacy of autologous vertebral BMA (vBMA) clot as a three-dimensional and multifunctional bioscaffold in instrumented posterior lumbar fusion.

Methods

Ten consecutive patients with an indication of multilevel (≤5) posterior spinal fusion due to lumbar spine degenerative diseases were included in the study and treated with vBMA. Clinical outcomes were assessed using the Visual Analog Scale (VAS), Oswestry Disability Index (ODI), and EuroQoL-5L (EQ-5L) preoperatively and at 3 months and 12 months after spinal fusion. Bone fusion quality was evaluated at the 12-month follow-up using the Brantigan classification on radiography (XR) imaging. Bone density was measured on computed tomography (CT) scans at 6 and 12 months of follow-up visits at the intervertebral arches and intervertebral joint areas and expressed in Hounsfield unit (HU).

Results

The results indicate a successful posterolateral fusion rate of approximately 100% (considering levels with C, D, and E grades according to the Brantigan classification) at the 12-month follow-up, along with an increase in bone density from 6 to 12 months of follow-up. An improvement in the quality of life and health status following surgery, as assessed by clinical scores (ODI, VAS, and EQ-5L), was also observed as early as 3 months postsurgery. No adverse events related to the vBMA clot were reported.

Conclusion

This prospective pilot study demonstrates the effectiveness and safety profile of vBMA clot as an advanced bioscaffold capable of achieving posterior lumbar fusion in the treatment of degenerative spine diseases. This lays the groundwork for a larger randomized clinical study.
An omics review and perspective of researches on intrahepatic cholestasis of pregnancy
Authors: Wang Min , Chen Lingyan , Li Jingyang , You Yilan , Qian Zhiwen , Liu Jiayu , Jiang Ying , Zhou Tao , Gu Ying , Zhang Yan
Published: January 2024

Intrahepatic cholestasis of pregnancy (ICP) is one of the common pregnancy complications that may threaten the health of both pregnant women and their fetuses. Hence, it is of vital importance to identify key moleculars and the associated functional pathways of ICP, which will help us to better understand the pathological mechanisms as well as to develop precise clinical biomarkers. The emerging and developing of multiple omics approaches enable comprehensive studies of the genome, transcriptome, proteome and metabolome of clinical samples. The present review collected and summarized the omics based studies of ICP, aiming to provide an overview of the current progress, limitations and future directions. Briefly, these studies covered a broad range of research contents by the comparing of different experimental groups including ICP patients, ICP subtypes, ICP fetuses, ICP models and other complications. Correspondingly, the studied samples contain various types of clinical samples, in vitro cultured tissues, cell lines and the samples from animal models. According to the main research objectives, we further categorized these studies into two groups: pathogenesis and diagnosis analyses. The pathogenesis studies identified tens of functional pathways that may represent the key regulatory events for the occurrence, progression, treatment and fetal effects of ICP. On the other hand, the diagnosis studies tested more than 40 potential models for the early-prediction, diagnosis, grading, prognosis or differential diagnosis of ICP. Apart from these achievements, we also evaluated the limitations of current studies, and emphasized that many aspects of clinical characteristics, sample processing, and analytical method can greatly affect the reliability and repeatability of omics results. Finally, we also pointed out several new directions for the omics based analyses of ICP and other perinatal associated conditions in the future.
Zinc homeostasis and redox alterations in obesity
Authors: Franco Cristina , Canzoniero Lorella Maria Teresa
Published: January 2024

Impairment of both cellular zinc and redox homeostasis is a feature of several chronic diseases, including obesity. A significant two-way interaction exists between redox metabolism and the relatively redox-inert zinc ion. Redox metabolism critically influences zinc homeostasis and controls its cellular availability for various cellular functions by regulating zinc exchange from/to zinc-binding proteins. Zinc can regulate redox metabolism and exhibits multiple pro-antioxidant properties. On the other hand, even minor disturbances in zinc status and zinc homeostasis affect systemic and cellular redox homeostasis. At the cellular level, zinc homeostasis is regulated by a multi-layered machinery consisting of zinc-binding molecules, zinc sensors, and two selective families of zinc transporters, the Zinc Transporter (ZnT) and Zrt, Irt-like protein (ZIP). In the present review, we summarize the current state of knowledge on the role of the mutual interaction between zinc and redox homeostasis in physiology and pathophysiology, pointing to the role of zinc in the alterations responsible for redox stress in obesity. Since zinc transporters primarily control zinc homeostasis, we describe how changes in the expression and activity of these zinc-regulating proteins are associated with obesity.
Sirtuins mediate mitochondrial quality control mechanisms: a novel therapeutic target for osteoporosis
Authors: Zhang Tianchi , Wang Lining , Duan Xiping , Niu Yuanyuan , Li Muzhe , Yun Li , Sun Haitao , Ma Yong , Guo Yang
Published: January 2024

Mitochondria plays a role in cell differentiation and apoptosis processes. Maintaining mitochondrial function is critical, and this involves various aspects of mitochondrial quality control such as protein homeostasis, biogenesis, dynamics, and mitophagy. Osteoporosis, a metabolic bone disorder, primarily arises from two factors: the dysregulation between lipogenic and osteogenic differentiation of aging bone marrow mesenchymal stem cells, and the imbalance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption. Mitochondrial quality control has the potential to mitigate or even reverse the effects. Among the Sirtuin family, consisting of seven Sirtuins (SIRT1-7), SIRT1-SIRT6 play a crucial role in maintaining mitochondrial quality control. Additionally, SIRT1, SIRT3, SIRT6, and SIRT7 are directly involved in normal bone development and homeostasis by modulating bone cells. However, the precise mechanism by which these Sirtuins exert their effects remains unclear. This article reviews the impact of various aspects of mitochondrial quality control on osteoporosis, focusing on how SIRT1, SIRT3, and SIRT6 can improve osteoporosis by regulating mitochondrial protein homeostasis, biogenesis, and mitophagy. Furthermore, we provide an overview of the current state of clinical and preclinical drugs that can activate Sirtuins to improve osteoporosis. Specific Sirtuin-activating compounds are effective, but further studies are needed. The findings of this study may offer valuable insights for future research on osteoporosis and the development of clinical prevention and therapeutic target strategies.
Melatonin as an anti-stress signal: effects on an acute stress model and direct actions on interrenal tissue in goldfish
Authors: Azpeleta Clara , Delgado Mª Jesús , Metz Juriaan R. , Flik Gert , de Pedro Nuria
Published: January 2024

Background

Melatonin is a key hormone in regulation of circadian rhythms, and involved in many rhythmic functions, such as feeding and locomotor activity. Melatonin reportedly counteracts stress responses in many vertebrates, including fish. However, targets for this action of melatonin and underlying mechanisms remain unknown.

Results

This study reports potential anti-stress properties of melatonin in goldfish (Carassius auratus), with a focus on its effect on plasma cortisol, food intake, and locomotor activity, all of them involved in the responses to stress exposure. Indeed, acute injection of melatonin counteracted stress-induced hypercortisolinemia and reduced food intake. The reduced locomotor activity following melatonin treatment suggests a possible sedative role in fish. To assess whether this anti-stress effects of melatonin involve direct actions on interrenal tissue, in vitro cultures of head kidney (containing the interrenal cortisol-producing tissue) were carried out in presence of ACTH, melatonin, and luzindole, an antagonist of melatonin receptors. Melatonin in vitro reduced ACTH-stimulated cortisol release, an effect attenuated by luzindole; this suggests the presence of specific melatonin receptors in interrenal tissue.

Conclusions

Our data support a role for melatonin as an anti-stress signal in goldfish, and suggest that the interrenal tissue of teleosts may be a plausible target for melatonin action decreasing cortisol production.
The impact of 17β-estradiol on the estrogen-deficient female brain: from mechanisms to therapy with hot flushes as target symptoms
Authors: Prokai-Tatrai Katalin , Prokai Laszlo
Published: January 2024

Sex steroids are essential for whole body development and functions. Among these steroids, 17β-estradiol (E2) has been known as the principal “female” hormone. However, E2’s actions are not restricted to reproduction, as it plays a myriad of important roles throughout the body including the brain. In fact, this hormone also has profound effects on the female brain throughout the life span. The brain receives this gonadal hormone from the circulation, and local formation of E2 from testosterone via aromatase has been shown. Therefore, the brain appears to be not only a target but also a producer of this steroid. The beneficial broad actions of the hormone in the brain are the end result of well-orchestrated delayed genomic and rapid non-genomic responses. A drastic and steady decline in circulating E2 in a female occurs naturally over an extended period of time starting with the perimenopausal transition, as ovarian functions are gradually declining until the complete cessation of the menstrual cycle. The waning of endogenous E2 in the blood leads to an estrogen-deficient brain. This adversely impacts neural and behavioral functions and may lead to a constellation of maladies such as vasomotor symptoms with varying severity among women and, also, over time within an individual. Vasomotor symptoms triggered apparently by estrogen deficiency are related to abnormal changes in the hypothalamus particularly involving its preoptic and anterior areas. However, conventional hormone therapies to “re-estrogenize” the brain carry risks due to multiple confounding factors including unwanted hormonal exposure of the periphery. In this review, we focus on hot flushes as the archetypic manifestation of estrogen deprivation in the brain. Beyond our current mechanistic understanding of the symptoms, we highlight the arduous process and various obstacles of developing effective and safe therapies for hot flushes using E2. We discuss our preclinical efforts to constrain E2’s beneficial actions to the brain by the DHED prodrug our laboratory developed to treat maladies associated with the hypoestrogenic brain.
Role of gonadotropin-releasing hormone 2 and its receptor in human reproductive cancers
Authors: Desaulniers Amy T. , White Brett R.
Published: January 2024

Gonadotropin-releasing hormone (GnRH1) and its receptor (GnRHR1) drive reproduction by regulating gonadotropins. Another form, GnRH2, and its receptor (GnRHR2), also exist in mammals. In humans, GnRH2 and GnRHR2 genes are present, but coding errors in the GnRHR2 gene are predicted to hinder full-length protein production. Nonetheless, mounting evidence supports the presence of a functional GnRHR2 in humans. GnRH2 and its receptor have been identified throughout the body, including peripheral reproductive tissues like the ovary, uterus, breast, and prostate. In addition, GnRH2 and its receptor have been detected in a wide number of reproductive cancer cells in humans. Notably, GnRH2 analogues have potent anti-proliferative, pro-apoptotic, and/or anti-metastatic effects on various reproductive cancers, including endometrial, breast, placental, ovarian, and prostate. Thus, GnRH2 is an emerging target to treat human reproductive cancers.
Computing task allocation for power Internet of Things including renewable energy sources
Authors: Yang Xianfei , Yu Xiang , Li Xiang
Published: January 2024

Power Internet of Things (PIoT) is the key technology to build a new power system based on new energy. Focusing on the problem that the large amount of data leads to the long computation delay of cloud computing in the operation control process of PIoT including renewable energy sources, this paper establishes a cloud-edge-end collaborative optimization calculation model for PIoT based on edge computation. Combined with data collected by a variety of smart terminal devices (STDs) arranged around the power generation equipment, the edge computation framework of PIoT is analyzed, and a computing task allocation model based on minimizing average system latency is established. The corresponding simulation model is built for simulation verification. Compared with other baseline schemes, it has been demonstrated that the average system latency of all tasks can be significantly decreased by using the proposed optimization scheme.
An ultra-short-term forecasting method for multivariate loads of user-level integrated energy systems in a microscopic perspective: based on multi-energy spatio-temporal coupling and dual-attention mechanism
Authors: Yin Xiucheng , Gao Zhengzhong , Cheng Yumeng , Hao Yican , You Zhenhuan
Published: January 2024

An ultra-short-term multivariate load forecasting method under a microscopic perspective is proposed to address the characteristics of user-level integrated energy systems (UIES), which are small in scale and have large load fluctuations. Firstly, the spatio-temporal correlation of users’ energy use behavior within the UIES is analyzed, and a multivariate load input feature set in the form of a class image is constructed based on the various types of load units. Secondly, in order to maintain the feature independence and temporal integrity of each load during the feature extraction process, a deep neural network architecture with spatio-temporal coupling characteristics is designed. Among them, the multi-channel parallel convolutional neural network (MCNN) performs independent spatial feature extraction of the 2D load component pixel images at each moment in time, and feature fusion of various types of load features in high dimensional space. A bidirectional long short-term memory network (BiLSTM) is used as a feature sharing layer to perform temporal feature extraction on the fused load sequences. In addition, a spatial attention layer and a temporal attention layer are designed in this paper for the original input load pixel images and the fused load sequences, respectively, so that the model can better capture the important information. Finally, a multi-task learning approach based on the hard sharing mechanism achieves joint prediction of each load. The measured load data of a UIES is analyzed as an example to verify the superiority of the method proposed in this paper.
Genetically based variation in heat tolerance covaries with climate in a globally important disease vector
Authors: Orlinick Benjamin L. , Smith Angela , Medley Kim A. , Westby Katie M.
Published: January 2024

Introduction

Vector-borne diseases (VBDs) are an increasingly important global health concern in the face of climate change. Understanding the ecology and evolution of vector species is critical to predicting and combating VBD. Vectorial capacity models, used to forecast disease transmission, traditionally assume traits are constant among populations, and little is known about whether different vector populations vary in thermal tolerance. To test for geographic variation in upper thermal tolerance, we determined the critical thermal maximum (CTmax) of Aedes albopictus, a globally distributed mosquito and competent vector for many viruses including West Nile, chikungunya, and dengue.

Methods

We studied CTmax for eight different populations spanning four climate zones across the Eastern United States using common garden experiments to isolate genetic variation. To explore potential drivers of this variation we then tested the relationship between climatic variables measured near each population source and CTmax.

Results

We found significant differences across populations for both adults and larvae, and these differences were more pronounced for adults. Larvae had higher CTmax values compared to adults. Several climatic variables improved models of CTmax for both adults and larvae including mean and max seasonal temperature, annual precipitation, and relative humidity. Annual precipitation appears to be particularly important and has a positive linear relationship with CTmax.

Discussion

The differences between life stages likely result from different selection pressures experienced in their terrestrial and aquatic habitats. Importantly, the assumption that mosquito populations within a species have the same upper thermal limits does not hold in this case, thus it is important to use population-specific CTmax values, among other important physiological parameters that may vary, to more accurately model and forecast VBDs.
A novel feeding platform design for behavioral research on wild Tanimbar corellas (Cacatua goffiniana)
Authors: O’Hara Mark , Auersperg Alice M. I. , Prawiradilaga Dewi M. , Huber Ludwig , Mioduszewska Berenika
Published: January 2024

The study of animal behaviour and cognition would not be complete without investigations of wild, free-ranging individuals in their natural environment. However, direct observations of species living in dense habitats can be challenging, leading many studies to focus on attracting target species to pre-selected, monitored locations baited with food. So far, researchers have rarely applied this approach to studying wild psittacines – an avian group of high scientific interest due to their advanced cognitive skills and conservation threats. We developed hoistable feeding platforms and tested their suitability for reliably attracting groups of wild Tanimbar corellas (hereafter: Goffins), opportunistic feeding generalists well-known for their advanced cognitive skills. To this end, we recorded the visitation rates of free-ranging groups at feeding platforms temporarily baited with dry corn. Moreover, we investigated the effects of several weather-related measures, the temporal distribution of foraging activity, and the effect of conspecific contact calls (playback stimulus). The results indicate that Goffins accepted the feeding platforms and reliably aggregated within their natural daily foraging bouts. While group size during visitation bouts depended on rainfall and resource abundance, platforms were still regularly visited by fewer, possibly locally residing individuals. These results provide the first systematic investigation of the temporal distribution and social dynamics of foraging bouts in wild Goffins. This study presents a novel method for reliably attracting wild opportunistic feeding generalist psittacines to a standardised, monitored location. It provides a suitable approach for observing foraging aggregations and, in the future, testing the problem-solving abilities of free-ranging Goffins in their natural habitat.
Reproductive phenology of the Chesapeake Bay blue crab population in a changing climate
Authors: Schneider Alexandra K. , Fabrizio Mary C. , Lipcius Romuald N.
Published: January 2024

Global temperatures are rising across marine ecosystems in response to climate change. Marine and estuarine-dependent species including the blue crab, Callinectes sapidus, may adapt to warming temperatures phenologically, by shifting the seasonal timing of biological events, such as reproduction. In Chesapeake Bay, average water temperatures have risen by an average 0.02°C per year since the 1980s. Extension of the blue crab spawning season, through earlier onset and later conclusion, may augment annual brood production and alter the efficacy of management strategies. The duration of the potential spawning season from 1985 to 2019 was assessed using degree days, and the observed spawning season from 1995 to 2019 was assessed using the occurrence of ovigerous crabs from the Virginia Institute of Marine Science Trawl Survey in the James River and in the mainstem of lower Chesapeake Bay. Spawning degree days (SDD) and reproductive degree days (RDD) were defined using minimum temperatures of 19°C and 12°C, respectively. The mean duration of the potential spawning season increased by 25% in SDD and 10% in RDD between 1985 and 2019 in the James River and lower Chesapeake Bay, respectively. This progressive expansion of the potential spawning season was not, however, reflected in the observed spawning season. Rather, the onset, conclusion, and duration of the observed spawning season were variable over the time series. The observed month of onset was driven by RDD in spring, whereby spawning began earlier during warmer springs. The spawning conclusion date was driven by the onset of spawning, rather than Fall temperature, such that the duration of the observed spawning season and, therefore, annual brood production did not change over time. In Chesapeake Bay, the spawning stock is protected by a sanctuary that is closed to fishing from mid-May to mid-September during the putative spawning season. An earlier start to the spawning season during warmer springs, as seen in recent years, is expected to reduce the efficacy of the spawning sanctuary and intensify exploitation of the spawning stock, without enhancing brood production, thereby reducing reproductive output of the blue crab population in Chesapeake Bay.
Edaphic mesofauna responses to land use change for sugarcane cultivation: insights from contrasting soil textures
Authors: Vanolli Beatriz S. , de Andrade Nariane , Canisares Lucas Pecci , Franco André. L. C. , Pereira Arthur P. A. , Cherubin Maurício R.
Published: January 2024

Land use change (LUC), specifically the cultivation of monoculture sugarcane, can negatively impact soil biodiversity, leading to a decline in soil health and ecosystem functioning. However, while studies focusing on macrofauna and microorganisms are more frequent in the literature, the impacts of LUC on mesofauna are still little known. Therefore, this study aimed to investigate the impacts of the predominant LUC for sugarcane production in Brazil on the diversity of edaphic mesofauna in soils with contrasting textures. In addition, we assessed correlations between biodiversity and soil properties chemical, biological, and physical attributes. We took samples from two sites (clayey and sandy soils) in southeastern Brazil. The sequence of LUC included i) native vegetation (NV), ii) pasture (PA), iii) sugarcane (SC), and iv) sugarcane ratoon (SCr). In the rainy season, monoliths (25 x 25 x 10 cm), soil samples were collected at 0-10, 10-20, and 20-30 cm to assess soil mesofauna, soil chemical (pH, soil organic matter, phosphorus, sulfur, potassium, calcium, magnesium, potential acidity, cation exchange capacity), physical (soil porosity) and biological (microbial biomass carbon and nitrogen) properties. The mesofauna taxonomic groups were quantified after the classification. Briefly, a total of 22 taxonomic groups were classified. The most predominant groups were non-oribatid mites, oribatid mites, and Collembola. Richness and abundance were lower in the three land uses studied (PA, SCr, SC) compared to the intercept (NV). In clayey soil, diversity decreased from NV to PA (-0.68 ± 0.27) and SC (-0.55 ± 0.27) but not to SCr. In sandy soil, land use significantly impacted the mesofauna diversity and evenness index, significantly reducing these indexes in SCr in relation to NV. Although land use change towards more intensified systems resulted in a loss of richness and abundance of soil mesofauna, sugarcane cultivation over the years can recover the diversity of mesofauna in clay-textured soils. These results provide a scientific background to better understand the LUC effects on sugarcane cultivation and support the establishment of sustainable practices that enhance soil health and biodiversity in different soil textures. This study highlights the need for tailored land management considering soil texture and biodiversity for improved ecosystem services.
Exploring the influence of DNA methylation and single nucleotide polymorphisms of the Myostatin gene on growth traits in the hybrid grouper (Epinephelus fuscoguttatus (female) × Epinephelus polyphekadion (male))
Authors: Cao Liu , Ma Jun , Chen Pan , Hou Xingrong , Yang Ning , Lu Yan , Huang Hai
Published: January 2024

Investigations into the correlation between growth characteristics and DNA methylation levels, along with genetic variations, can provide fundamental insights to enhance growth performance in groupers. The Myostatin (mstn) gene plays a vital role in regulating skeletal muscle development and growth. This study scrutinized the DNA methylation levels of the mstn gene across hybrid groupers (E. fuscoguttatus (♀) × E. polyphekadion (♂)) and their parental species, to evaluate its impact on growth attributes in grouper fish. The nucleotide sequence of the mstn gene was directly sequenced in the hybrid grouper, exhibiting different growth performance to identify the single nucleotide polymorphisms (SNPs) of the mstn gene and explore their correlation with growth characteristics. The findings revealed no significant differences in global DNA methylation levels within muscle tissue among the hybrid grouper and parents. However, significant differences in DNA methylation sites were discovered between the hybrid grouper and E. polyphekadion at sites 824 and 1521 (located at exon 2 and intron 2, respectively), and between E. fuscoguttatus and E. polyphekadion at site 1521. These variations could potentially influence the mRNA expression of the mstn gene. The study also identified that SNP g.1003 T > C in exon 2 of the mstn gene was significantly associated with various growth traits including body weight, total length, body length, head length, caudal peduncle height, and body height (p < 0.01). Specimens with the TT genotype at site 1003 demonstrated superior growth performance compared to those with the TC genotype. Furthermore, microstructural analyses of muscle tissue showed that the average area and diameter of muscle fibers in TT genotype individuals were significantly greater than those in TC genotype individuals. Therefore, this research provides robust evidence linking the DNA methylation level and polymorphisms of the mstn gene with growth traits, which could be beneficial for grouper breeding programs.
Genetic interactions of schizophrenia using gene-based statistical epistasis exclusively identify nervous system-related pathways and key hub genes
Authors: Periyasamy Sathish , Youssef Pierre , John Sujit , Thara Rangaswamy , Mowry Bryan J.
Published: January 2024

Background: The relationship between genotype and phenotype is governed by numerous genetic interactions (GIs), and the mapping of GI networks is of interest for two main reasons: 1) By modelling biological robustness, GIs provide a powerful opportunity to infer compensatory biological mechanisms via the identification of functional relationships between genes, which is of interest for biological discovery and translational research. Biological systems have evolved to compensate for genetic (i.e., variations and mutations) and environmental (i.e., drug efficacy) perturbations by exploiting compensatory relationships between genes, pathways and biological processes; 2) GI facilitates the identification of the direction (alleviating or aggravating interactions) and magnitude of epistatic interactions that influence the phenotypic outcome. The generation of GIs for human diseases is impossible using experimental biology approaches such as systematic deletion analysis. Moreover, the generation of disease-specific GIs has never been undertaken in humans.

Methods: We used our Indian schizophrenia case-control (case-816, controls-900) genetic dataset to implement the workflow. Standard GWAS sample quality control procedure was followed. We used the imputed genetic data to increase the SNP coverage to analyse epistatic interactions across the genome comprehensively. Using the odds ratio (OR), we identified the GIs that increase or decrease the risk of a disease phenotype. The SNP-based epistatic results were transformed into gene-based epistatic results.

Results: We have developed a novel approach by conducting gene-based statistical epistatic analysis using an Indian schizophrenia case-control genetic dataset and transforming these results to infer GIs that increase the risk of schizophrenia. There were ∼9.5 million GIs with a p-value ≤ 1 × 10−5. Approximately 4.8 million GIs showed an increased risk (OR > 1.0), while ∼4.75 million GIs had a decreased risk (OR <1.0) for schizophrenia.

Conclusion: Unlike model organisms, this approach is specifically viable in humans due to the availability of abundant disease-specific genome-wide genotype datasets. The study exclusively identified brain/nervous system-related processes, affirming the findings. This computational approach fills a critical gap by generating practically non-existent heritable disease-specific human GIs from human genetic data. These novel datasets can train innovative deep-learning models, potentially surpassing the limitations of conventional GWAS.
Fight or flight: reimagining Charles Jesse “Buffalo” Jones' conservation efforts through a bison's embodied perspective
Authors: Wilson Michelle
Published: January 2024

In this speculative historical essay, I employ Buffalo Jones' records to expand upon who can have a voice in the archive, thereby undermining the anthropocentrism inherent in the chronicling of bison conservation. Indeed, this work of “fictocriticism” deploys empathy to recenter the more-than-human voice and ironically uses the observations of the bison's tormentor to move beyond a simplistic anthropomorphic representation. The essay briefly introduces Jones and his contentious legacy as a murderer of Indigenous peoples, buffalo hunter and, later, a central figure in early bison conservation. The essay then shifts into a first-person account of the pursuit and capture of the last remaining southern plains bison from the Texas Panhandle from a cow's embodied perspective. The bison's first-person perspective as one of Jones' prey brings an immediacy to a history that has often been retold to center man's mastery and supremacy. Finally, this essay employs footnotes as a critical intervention by connecting the speculative narrative to Jones' written accounts in published journals. These two narrative approaches demonstrate the significance of bison kinship and how anthropocentrism and white supremacy's entangled ideologies blinded Jones to the worthiness of these others' lifeways. The written submission is accompanied by two audio artworks based on this essay. In creating affective, sound-designed audio works, I have intentionally extracted the archival-research-based narrative from a white supremacist, a patriarchal written tradition for critical purposes.
In vitro B cell experiments explore the role of CD24, CD38, and energy metabolism in ME/CFS
Authors: Armstrong Christopher W. , Mensah Fane F. K. , Leandro Maria J. , Reddy Venkat , Gooley Paul R. , Berkovitz Saul , Cambridge Geraldine
Published: January 2024

Introduction

Disturbances of energy metabolism contribute to the clinical manifestations of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Previously, we found that B cells from ME/CFS patients have an increased expression of CD24, a modulator of many cellular functions including those of cell stress. The relative ability of B cells from ME/CFS patients and healthy controls (HC) to respond to rapid changes in energy demand was compared.

Methods

CD24, the ectonucleotidases CD39 and CD73, the NAD-degrading enzyme CD38, and mitochondrial mass (MM) were measured following cross-linking of the B cell receptor and costimulation with either T-cell-dependent or Toll-like-receptor-9-dependent agonists. The levels of metabolites consumed/produced were measured using 1H-NMR spectroscopy and analyzed in relation to cell growth and immunophenotype.

Results

Proliferating B cells from patients with ME/CFS showed a lower mitochondrial mass and a significantly increased usage of essential amino acids compared with those from HC, with a significantly delayed loss of CD24 and an increased expression of CD38 following stimulation.

Discussion

The immunophenotype results suggested the triggering of a stress response in ME/CFS B cells associated with the increased usage of additional substrates to maintain necessary ATP levels. Disturbances in energy metabolism in ME/CFS B cells were thus confirmed in a dynamic in vitro model, providing the basis for further mechanistic investigations.
Transmission characteristics and inactivated vaccine effectiveness against transmission of the SARS-CoV-2 Omicron BA.2 variant in Shenzhen, China
Authors: He Xiaofeng , Liao Yuxue , Liang Yuanhao , Yu Jiexin , Gao Wei , Wan Jia , Liao Yi , Su Jiao , Zou Xuan , Tang Shixing
Published: January 2024

We conducted a retrospective cohort study to evaluate the transmission risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron BA.2 variant and the effectiveness of inactivated COVID-19 vaccine boosters in Shenzhen during a BA.2 outbreak period from 1 February to 21 April 2022. A total of 1,248 individuals were infected with the BA.2 variant, and 7,855 close contacts were carefully investigated. The risk factors for the high secondary attack rate of SARS-CoV-2 infection were household contacts [adjusted odds ratio (aOR): 1.748; 95% confidence interval (CI): 1.448, 2.110], younger individuals aged 0–17 years (aOR: 2.730; 95% CI: 2.118, 3.518), older persons aged ≥60 years (aOR: 1.342; 95% CI: 1.135, 1.588), women (aOR: 1.442; 95% CI: 1.210, 1.718), and the subjects exposed to the post-onset index cases (aOR: 8.546; 95% CI: 6.610, 11.050), respectively. Compared with the unvaccinated and partially vaccinated individuals, a relatively low risk of secondary attack was found for the individuals who received booster vaccination (aOR: 0.871; 95% CI: 0.761, 0.997). Moreover, a high transmission risk was found for the index cases aged ≥60 years (aOR: 1.359; 95% CI: 1.132, 1.632), whereas a relatively low transmission risk was observed for the index cases who received full vaccination (aOR: 0.642; 95% CI: 0.490, 0.841) and booster vaccination (aOR: 0.676; 95% CI: 0.594, 0.770). Compared with full vaccination, booster vaccination of inactivated COVID-19 vaccine showed an effectiveness of 24.0% (95% CI: 7.0%, 37.9%) against BA.2 transmission for the adults ≥18 years and 93.7% (95% CI: 72.4%, 98.6%) for the adults ≥60 years, whereas the effectiveness was 51.0% (95% CI: 21.9%, 69.3%) for the individuals of 14 days to 179 days after booster vaccination and 51.2% (95% CI: 37.5%, 61.9%) for the non-household contacts. The estimated mean values of the generation interval, serial interval, incubation period, latent period, and viral shedding period were 2.7 days, 3.2 days, 2.4 days, 2.1 days, and 17.9 days, respectively. In summary, our results confirmed that the main transmission route of Omicron BA.2 subvariant was household contact, and booster vaccination of the inactivated vaccines was relatively effective against BA.2 subvariant transmission in older people.