bluuebunny/crossref_metadata_2025 huggingface dataset:
From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization of Eragrostis curvula
Authors: Carballo Jose , Bellido Andrés Martin , Selva Juan Pablo , Zappacosta Diego , Gallo Cristian Andres , Albertini Emidio , Caccamo Mario , Echenique Viviana
Published: March 2023

Introduction

In Eragrostis curvula, commonly known as weeping lovegrass, a synthetic diploidization event of the facultative apomictic tetraploid Tanganyika INTA cv. originated from the sexual diploid Victoria cv. Apomixis is an asexual reproduction by seeds in which the progeny is genetically identical to the maternal plant.

Methods

To assess the genomic changes related to ploidy and to the reproductive mode occurring during diploidization, a mapping approach was followed to obtain the first E. curvula pangenome assembly. In this way, gDNA of Tanganyika INTA was extracted and sequenced in 2x250 Illumina pair-end reads and mapped against the Victoria genome assembly. The unmapped reads were used for variant calling, while the mapped reads were assembled using Masurca software.

Results

The length of the assembly was 28,982,419 bp distributed in 18,032 contigs, and the variable genes annotated in these contigs rendered 3,952 gene models. Functional annotation of the genes showed that the reproductive pathway was differentially enriched. PCR amplification in gDNA and cDNA of Tanganyika INTA and Victoria was conducted to validate the presence/absence variation in five genes related to reproduction and ploidy. The polyploid nature of the Tanganyika INTA genome was also evaluated through the variant calling analysis showing the single nucleotide polymorphism (SNP) coverage and allele frequency distribution with a segmental allotetraploid pairing behavior.

Discussion

The results presented here suggest that the genes were lost in Tanganyika INTA during the diploidization process that was conducted to suppress the apomictic pathway, affecting severely the fertility of Victoria cv.
Melatonin-mediated endogenous nitric oxide coordinately boosts stability through proline and nitrogen metabolism, antioxidant capacity, and Na+/K+ transporters in tomato under NaCl stress
Authors: Ghorbani Abazar , Pishkar Leila , Saravi Kobra Valed , Chen Moxian
Published: March 2023

The interactions between nitric oxide (NO) and melatonin in alleviating sodium chloride (NaCl) toxicity in plants are poorly comprehended. Here, the associations between the exogenous application of melatonin and endogenous NO levels in inducing tomato seedlings’ defense response during NaCl toxicity were investigated. The results indicated that the application of melatonin (150 μM) increased height (23.7%) and biomass (32.2%), improved chlorophyll (a (137%) and b (92.8%)), and proline metabolisms, and reduced the contents of superoxide anion radicals (49.6%), hydrogen peroxide (31.4%), malondialdehyde (38%), and electrolyte leakage (32.6%) in 40-day-old tomato seedlings grown under NaCl (150 mM) treatment. Melatonin increased the antioxidant defense system in NaCl-stressed seedlings by increasing the activity of the antioxidant enzymes. Melatonin also improved N metabolism and endogenous NO content in NaCl-stressed seedlings by upregulating the activity of enzymes implicated in N assimilation. Furthermore, melatonin improved ionic balance and reduced Na content in NaCl-exposed seedlings by upregulating the expression of genes involved in K/Na ratio homeostasis (NHX1-4) and increasing the accumulation of mineral nutrients (P, N, Ca, and Mg). However, the addition of cPTIO (100 μM; an NO scavenger) reversed the beneficial impacts of melatonin, indicating the effective function of NO in melatonin-induced defense mechanisms in NaCl-stressed tomato seedlings. Therefore, our results revealed that melatonin improves the tolerance of tomato plants during NaCl toxicity by mediating internal NO.
Postharvest light-induced flavonoids accumulation in mango (Mangifera indica L.) peel is associated with the up-regulation of flavonoids-related and light signal pathway genes
Authors: Zhu Wencan , Wu Hongxia , Yang Chengkun , Shi Bin , Zheng Bin , Ma Xiaowei , Zhou Kaibing , Qian Minjie
Published: March 2023

Introduction

Flavonoids are important secondary metabolites in plants and light is a crucial environmental factor regulating flavonoids biosynthesis. However, effect of light on the different flavonoids compositions accumulation in mango and the relevant molecular mechanism still need to be clarified.

Methods

In this study, green-mature fruits of red mango cultivar ‘Zill’ were subjected to postharvest light treatment, and fruit peel color, total soluble solids content, total organic acid, and firmness of flesh were measured. The flavonoids metabolites profile, and the expression of flavonoids-related genes and light signal pathway genes were also analyzed.

Results

Results showed that light treatment promoted the red coloration of fruit peel and increased the total soluble solids content and firmness of flesh. The concentration of flavonols, proanthocyanidins and anthocyanins, and expression of key flavonoids biosynthetic genes including MiF3H, MiFLS, MiLAR, MiANS, MiUFGT1, and MiUFGT3 were significantly induced by light. The MYBs regulating flavonols and proanthocyanidins, i.e. MiMYB22 and MiMYB12, as well as the key light signal pathway transcription factors (TFs) MiHY5 and MiHYH, were identified in mango. The transcription of MiMYB1, MiMYB12, MiMYB22, MiHY5 and MiHYH was up-regulated by light.

Discussion

Our results provide a postharvest technology to improve mango fruit appearance quality, and are helpful to reveal the molecular mechanism of light-induced flavonoids biosynthesis in mango.
Long-term cattle manure addition enhances soil-available phosphorus fractions in subtropical open-field rotated vegetable systems
Authors: Mao Yanting , Hu Wei , Li Yongmei , Li Yuan , Lei Baokun , Zheng Yi
Published: March 2023

Introduction

Evaluation of the changes in phosphorus (P) fractions (various P forms) and their availability at different soil layers is critical for enhancing P resource use efficiency, mitigating subsequent environmental pollution, and establishing a suitable manure application strategy. However, changes in P fractions at different soil layers in response to cattle manure (M), as well as a combined cattle manure and chemical fertilizer application (M+F), remain unclear in open-field vegetable systems. If the amount of annual P input remains the same, identifying which treatment would cause a higher phosphate fertilizer use efficiency (PUE) and vegetable yield while simultaneously reducing the P surplus is especially warranted.

Methods

Based on a long-term manure experiment that started in 2008, we used a modified P fractionation scheme to analyze P fractions at two soil layers for three treatments (M, M+F, and control without fertilizer application) in an open-field cabbage (Brassica oleracea) and lettuce (Lactuca sativa) system, and assessed the PUE and accumulated P surplus.

Results

The concentrations of the soil P fractions were higher in the 0–20-cm soil layer compared to the 20–40-cm layer, except for organic P (Po) and residual-P. M application significantly increased the inorganic P (Pi) (by 8.92%–72.26%) and the Po content (by 5.01%–61.23%) at the two soil layers. Compared with the control and M+F treatments, M significantly increased residual-P, Resin-P, and NaHCO3-Pi at both soil layers (by 31.9%–32.95%, 68.40%–72.60%, and 48.22%–61.04%), whereas NaOH-Pi and HCl-Pi at 0–20 cm were positively correlated with available P. Soil moderately labile-P was the predominant P component in the two soil layers (accounting for 59%–70%). With the same annual P input amount, M+CF created the highest vegetable yield (117.86 t ha-1), and PUE (37.88%) and M created the highest accumulated P surplus (128.80 kg ha-1yr-1).

Discussion

Collectively, a combined manure-chemical fertilizer application has great potential to yield a long-term positive outcome both in terms of vegetable productivity and environmental health in open-field vegetable systems. This highlights the methods’ benefits as a sustainable practice in subtropical vegetable systems. Specific attention should be given to a P balance to avoid excessive P input if a rational strategy for manure application is to be attained. This is especially the case for stem vegetables that require manure application and decreases the environmental risk of P loss in vegetable systems.
Efficacy of preharvest application of biocontrol agents against gray mold in grapevine
Authors: Altieri Valeria , Rossi Vittorio , Fedele Giorgia
Published: March 2023

The use of biocontrol agents (BCAs) represents a promising alternative to conventional methods for the management of gray mold in vineyards during the berry ripening stage. The main advantages of BCAs are the short preharvest interval and lack of chemical fungicide residues in wine. In this study, eight commercial BCAs (based on different Bacillus or Trichoderma species and strains, Aureobasidium pullulans, Metschnikowia fructicola, and Pythium oligandrum) and a reference fungicide (boscalid) were applied to a vineyard during berry ripening over three seasons to evaluate the dynamics over time in terms of their relative efficacies in gray mold control. At 1–13 days after application of BCAs to the berry surfaces in field conditions, the berries were collected and artificially inoculated with conidia of Botrytis cinerea under controlled laboratory conditions, and gray mold severity was observed after 7 days of incubation. Significant differences were observed in gray mold severity among years, according to the number of days the BCAs grew on the berry surface before B. cinerea inoculation, and the season by day interaction (altogether accounting for >80% of the experimental variance). The variability in BCA efficacy was closely related to the environmental conditions at the time of application and in the following days. Overall, the BCA efficacy increased with the degree days accumulated between BCA application in the vineyard and B. cinerea inoculation in the dry (no rain) periods (r = 0.914, P = 0.001). Rainfall and the associated drop in temperature caused a relevant reduction of BCA efficacy. These results demonstrate that BCAs are an effective alternative to conventional chemicals for the preharvest control of gray mold in vineyards. However, environmental conditions can considerably affect the BCA efficacy.
The impacts of COVID-19 on the relationship between perceived economic inequality and political action among socioeconomic classes
Authors: Vezzoli Michela , Mari Silvia , Valtorta Roberta Rosa , Volpato Chiara
Published: March 2023

Economic inequality qualifies as a structural characteristic leading to political action, albeit this relationship manifests differently across socioeconomic classes. COVID-19 pandemic has amplified existing economic inequalities in ways that increased social tensions and political unrest around the world. This research investigates the effect of COVID-19 personal impacts on the relationship between perceived economic inequality and individuals' political participation. An online survey was administered to an Italian representative sample of 1,446 people (51% women, mean age of 42.42 years,SD= 12.87). The questionnaire assessed the perceived economic inequality, the personal impacts of COVID-19 (i.e., on finance, mental health, and ability to procure resources), and individuals' involvement in political participation. Moderation analyses were conducted separately for different socioeconomic classes (i.e., lower, middle, and upper classes). Results showed that individuals who perceive greater economic inequality, while controlling for perceived wage gap, are more likely to take action, but only if they belong to the higher class. For lower-class individuals, perceiving greater inequality erodes political action. Interaction effects occurred mainly in the middle class and with COVID-19 impacts on resources procurement, which inhibits political action.
The associations between social environment and adolescents’ psychosomatic health: An ecological perspective
Authors: Huang Yi , Lu Jinjin , Širůček Jan
Published: March 2023

Objectives

It has been known that social environments are associated with adolescents’ health. However, the complex relationship between diverse types of social environments and adolescents’ psychosomatic heath remained unclear. Thus, using an ecological perspective, the current study aimed to examine the associations between social environment and adolescents’ psychosomatic health.

Methods

We used the data from the Health Behavior in School-aged Children (HBSC) project conducted in the Czech Republic in 2018. A total of 13377 observations were included.

Results

The region, as a macrosystem, could not explain the variance in adolescents’ psychological and somatic health. The quality of neighborhood environment (exosystem) was significantly related to adolescents’ psychological and somatic health. At the microsystem level, teacher support had stronger, family support had weaker, and peer support had no association with psychological and somatic health. At the mesosystem level, the interactions between family, teacher, and friend support were negligible for adolescents’ psychological and somatic health.

Conclusions

The results underscore the importance of teachers’ support and neighborhood environment for adolescents’ psychosomatic health. Therefore, the findings suggest the need to improve teacher-adolescent relationships and the neighborhood community quality.
The impact of the war in Ukraine on the perinatal period: Perinatal mental health for refugee women (pmh-rw) protocol
Authors: Rodríguez-Muñoz Maria F. , Chrzan-Dętkoś Magdalena , Uka Ana , García-López Helena S. , Krupelnytska Liudmyla , Morozova-Larina Olha , Vavilova Alona , Molotokas Antonina , Murawska Natalia , Le Huynh-Nhu
Published: March 2023

Background

The aim of the Perinatal Mental Health for Refugee Women (PMH-RW) Project is to investigate the impact of the war on perinatal mental health: anxiety, post-traumatic stress, depression, and birth trauma symptoms. It will also evaluate the factors that serve as protective elements for the development of these potential diagnoses (such as personality traits, social support, sociodemographic characteristics, and access to medical/mental health services).

Method

An international observational cohort study with baseline data is being assessed in Ukraine (for internal refugees) and several European Countries (for external refugees). The study includes as participants both pregnant women and those who have recently given birth (with babies up to 1 year old). The assessment includes measures on depression (EPDS), anxiety (GAD-7), experiences during birth (City Birth Questionnaire), post-traumatic stress symptoms [Impact of events scale—revised (PTSD-R)], Personality (10-Item Personality Inventory-TIPI), and a questionnaire for socio-demographic data which also such social support.

Conclusion

This study will provide needed information for determining the impact of the Ukrainian Crisis on perinatal mental health by studying potential risk and protective factors. The data collected will be used to inform policymakers with useful information that can be used in the development of plans to protect and promote the mental health of the perinatal refugees impacted by this event. Also, it is our hope that data collected from this study will serve to plant the seeds for further research regarding the impact of the crisis in Ukraine on the offspring and to analyze how these events are affecting further generations.

Clinical Trial Registration

ClinicalTrials.gov, Identifier: NCT05654987.
A comparative study of well-being, resilience, mindfulness, negative emotions, stress, and burnout among nurses after an online mind–body based intervention during the first COVID-19 pandemic crisis
Authors: Cepeda-Lopez Ana Carla , Solís Domínguez Leticia , Villarreal Zambrano Sofía , Garza-Rodriguez Iris Y. , del Valle Alejandra Cortes , Quiroga-Garza Angélica
Published: March 2023

Background

To mitigate against the possible adverse effects of stress among nurses due to the COVID-19 outbreak, we designed a 12-week mind–body based online intervention program to promote well-being and prevent stress-related disorders such as burnout. Our study aimed to compare the impact of the intervention on perception of stress, negative emotions, burnout, mindfulness, resilience, and well-being at pretest and 6 months post-intervention and to compare the effect among nurses working at two different hospitals.

Methods

We conducted an uncontrolled trial using a convenience sample of nurses working at two hospitals in Mexico: one designated to treat confirmed COVID-19 patients (COVID-hospital) and the other whose patients had a negative COVID-19 test on admission (Non COVID-hospital). The 12 week online intervention consisted of 36 mind–body based micropractices, with subjective well-being as the primary outcome. Secondary outcomes were health perception, resilience, mindfulness, negative emotions, stress, and burnout.

Results

A pretest survey was completed by 643 nurses. Of the remaining valid responses, 82% were women, with a mean age of 34.8 (SD = 8.95) years old. For the analysis two groups of nurses were sampled by cluster: a COVID-hospital group of 429 (67%) nurses, and a non-COVID Hospital group of 214 (33%) nurses. The proportion lost to follow-up was 71% at postest (n = 188) and 42% at 6 months follow-up (n = 371). At pretest, non-COVID hospital nurses had lower subjective well-being and higher burnout than their COVID hospital counterparts. At postest, non-COVID hospital nurses displayed more negative emotions than their COVID hospital peers. At 6 months post-intervention, nurses experienced improved mindfulness, reduced negative emotions and stress, but a decrease in subjective well-being and resilience. Nurses working at the non-COVID hospital had significantly higher mean scores for burnout than those working at the COVID hospital.

Conclusion

The results of our study suggest that our online mind–body interventions can help to reduce stress and negative emotions, yet the effects on subjective well-being and resilience are uncertain. Further research is needed to gain a better understanding of their potential mechanisms and the associated efforts of such online interventions.

Clinical Trial Registration

ClinicalTrials.gov, NCT05515172.
Smartwatch digital phenotypes predict positive and negative symptom variation in a longitudinal monitoring study of patients with psychotic disorders
Authors: Kalisperakis Emmanouil , Karantinos Thomas , Lazaridi Marina , Garyfalli Vasiliki , Filntisis Panagiotis P. , Zlatintsi Athanasia , Efthymiou Niki , Mantas Asimakis , Mantonakis Leonidas , Mougiakos Theodoros , Maglogiannis Ilias , Tsanakas Panayotis , Maragos Petros , Smyrnis Nikolaos
Published: March 2023

Introduction

Monitoring biometric data using smartwatches (digital phenotypes) provides a novel approach for quantifying behavior in patients with psychiatric disorders. We tested whether such digital phenotypes predict changes in psychopathology of patients with psychotic disorders.

Methods

We continuously monitored digital phenotypes from 35 patients (20 with schizophrenia and 15 with bipolar spectrum disorders) using a commercial smartwatch for a period of up to 14 months. These included 5-min measures of total motor activity from an accelerometer (TMA), average Heart Rate (HRA) and heart rate variability (HRV) from a plethysmography-based sensor, walking activity (WA) measured as number of total steps per day and sleep/wake ratio (SWR). A self-reporting questionnaire (IPAQ) assessed weekly physical activity. After pooling phenotype data, their monthly mean and variance was correlated within each patient with psychopathology scores (PANSS) assessed monthly.

Results

Our results indicate that increased HRA during wakefulness and sleep correlated with increases in positive psychopathology. Besides, decreased HRV and increase in its monthly variance correlated with increases in negative psychopathology. Self-reported physical activity did not correlate with changes in psychopathology. These effects were independent from demographic and clinical variables as well as changes in antipsychotic medication dose.

Discussion

Our findings suggest that distinct digital phenotypes derived passively from a smartwatch can predict variations in positive and negative dimensions of psychopathology of patients with psychotic disorders, over time, providing ground evidence for their potential clinical use.
The experiences of practitioners working with young people exposed to online sexual abuse
Authors: Quayle Ethel , Schwannauer Matthias , Varese Filippo , Cartwright Kim , Hewins William , Chan Cindy , Newton Alice , Chitsabesan Prathiba , Richards Cathy , Bucci Sandra
Published: March 2023

Introduction

This qualitative study explored healthcare professionals' current understanding of, and clinical practices related to, Online Child Sexual Abuse (OCSA).

Methods

Data were collected across two UK sites (Manchester and Edinburgh). Interviews and one focus group were held with 25 practitioners working in services offering clinical support to young people who have experienced OCSA. Thematic analysis of the data identified three overarching themes and 10 subthemes related to the research questions: (1) the breadth of the problem; (2) working with OCSA; and (3) the emotionally charged nature of OCSA.

Results

While practitioners recognized OCSA as problematic, they differed in how they conceptualized it. There was a heightened awareness of the role that sexual images played in OCSA and concerns about first-person-produced imagery by Children and Young People (CYP). Practitioners described a generational gap related to their technology use and that of the young people they worked with. Practitioners also described a paucity of referral pathways and concerns that there was no training available to them. Organizational barriers meant that questions about technology use were not routinely included in assessments and often there was reliance on young people making disclosures.

Discussion

Novel findings from this study were the psychological impacts that such cases had on practitioners, which may indicate a need for organizational support for staff as well as further training needs. Existing frameworks that help conceptualize and assess the role of technology as part of the ecology of the child may have great utility for practitioners.
Review of factors resulting in systemic biases in the screening, assessment, and treatment of individuals at clinical high-risk for psychosis in the United States
Authors: Bridgwater Miranda A. , Petti Emily , Giljen Maksim , Akouri-Shan LeeAnn , DeLuca Joseph S. , Rakhshan Rouhakhtar Pamela , Millar Caroline , Karcher Nicole R. , Martin Elizabeth A. , DeVylder Jordan , Anglin Deidre , Williams Raquel , Ellman Lauren M. , Mittal Vijay A. , Schiffman Jason
Published: March 2023

Background

Since its inception, research in the clinical high-risk (CHR) phase of psychosis has included identifying and exploring the impact of relevant socio-demographic factors. Employing a narrative review approach and highlighting work from the United States, sociocultural and contextual factors potentially affecting the screening, assessment, and service utilization of youth at CHR were reviewed from the current literature.

Results

Existing literature suggests that contextual factors impact the predictive performance of widely used psychosis-risk screening tools and may introduce systemic bias and challenges to differential diagnosis in clinical assessment. Factors reviewed include racialized identity, discrimination, neighborhood context, trauma, immigration status, gender identity, sexual orientation, and age. Furthermore, racialized identity and traumatic experiences appear related to symptom severity and service utilization among this population.

Conclusions

Collectively, a growing body of research from the United States and beyond suggests that considering context in psychosis-risk assessment can provide a more accurate appraisal of the nature of risk for psychosis, render more accurate results improving the field's prediction of conversion to psychosis, and enhance our understanding of psychosis-risk trajectories. More work is needed in the U.S. and across the globe to uncover how structural racism and systemic biases impact screening, assessment, treatment, and clinical and functional outcomes for those at CHR.
COVID-19 pandemic’s impact on networks of depression and anxiety in naturalistic transdiagnostic sample of outpatients with non-psychotic mental illness
Authors: Kim Shin Tae , Seo Jun Ho , Park Chun Il , Kim Se Joo , Kang Jee In
Published: March 2023

Background

The 2019 coronavirus disease (COVID-19) pandemic has caused an unprecedented disruption of daily lives and a mental health crisis. The present study examined how the depression and anxiety symptom network changed during the COVID-19 pandemic in a naturalistic transdiagnostic sample with non-psychotic mental illness.

Materials and methods

A total of 224 psychiatric outpatients before the pandemic and 167 outpatients during the pandemic were included in the study and were assessed for the Patient Health Questionnaire and the Beck Anxiety Inventory. The network of depression and anxiety symptoms before and during the pandemic were estimated separately and were assessed differences.

Results

The network comparison analysis showed a significant structural difference between the networks before and during the pandemic. Before the pandemic, the most central symptom in the network was feelings of worthlessness, while in the during pandemic network, somatic anxiety emerged as the most central node. Somatic anxiety, which showed the highest strength centrality during the pandemic, showed significantly increased correlation with suicidal ideation during the pandemic.

Limitations

The two cross-sectional network analyses of individuals at one point in time cannot demonstrate causal relationships among measured variables and cannot be assumed to generalize to the intraindividual level.

Conclusion

The findings indicate that the pandemic has brought a significant change in the depression and anxiety network and somatic anxiety may serve as a target for psychiatric intervention in the era of the pandemic.
Recent advances in the identification of related factors and preventive strategies of hip fracture
Authors: Yu Yaohui , Wang Yudan , Hou Xiaoli , Tian Faming
Published: March 2023

Hip fracture is the most devastating type of osteoporosis-related fracture, and is a major worldwide public health problem with a high socioeconomic burden, morbidity rate, and mortality rate. Thus, it is crucial to uncover the risk factors and protective factors to create a hip fracture prevention strategy. In addition to a briefly review of some well accepted risk and protective factors of hip fracture, this review mainly summarized the recent advances in the identification of emerging risk or protective factors for hip fracture, in terms of regional differences in medical services, diseases, drugs, mechanical load, neuromuscular mass, genes, blood types, cultural differences. This review provides a comprehensive review of the associated factors and effective prevention measures for hip fracture, and discusses issues that need further investigation. These issues include the determination of the influencing mechanism of risk factors triggering hip fracture and their interlinked correlation with other factors, as well as the confirmation or correction of emerging factors associated with hip fracture, particularly those that are still controversial. These recent findings will aid in optimizing the strategy for preventing hip fracture.
Psychosocial work environment stressors for school staff during the COVID-19 pandemic: Barriers and facilitators for supporting wellbeing
Authors: Rolf Liz R. , Vestal Liz , Moore Ashley C. , Lobb Dougherty Nikole , Mueller Nancy , Newland Jason G.
Published: March 2023

Introduction

After periods of remote and/or hybrid learning as a result of the COVID-19 global pandemic, the return to in-person learning has been beneficial for both students and teachers, but it has not been without challenges. This study was designed to assess the impact of the return to in-person learning on the school experience, and efforts made to ease the transition in furthering a positive in-person learning environment.

Materials and methods

We conducted a series of listening sessions with 4 stakeholder groups: students (n = 39), parents (n = 28), teachers/school staff (n = 41), and a combination of listening sessions and semi-structured interviews with building level and district administrators (n = 12), focusing on in-school experiences during the 2021–2022 school year amidst the COVID-19 pandemic. A primarily deductive qualitative analysis approach was employed to code the data followed by a primarily inductive thematic analysis, followed by thematic aggregation, thus providing depth and identification of nuances in the data.

Results

Three main themes emerged around school staff experiences: (1) increased levels of stress and anxiety manifested in key ways, including students' challenges with personal behavior management contributing to increased aggression and staff shortages; (2) school staff described key contributors to stress and anxiety, including feeling excluded from decision making and challenges with clear and consistent communication; and (3) school staff described key facilitators that supported their management of anxiety and stress, including adaptability, heightened attention and resources to wellbeing, and leveraging interpersonal relationships.

Discussion

School staff and students faced significant stress and anxiety during the 2021–2022 school year. Further exploration and identification of approaches to mitigate key contributors to increased stress and anxiety for school staff, along with increased opportunities for implementing key facilitators that were identified as important in managing and navigating the increased stress and anxiety offer valuable opportunities for helping to create a supportive work environment for school staff in the future.
Nature visits during the COVID-19 pandemic in Norway: Facilitators, motives, and associations with sociodemographic characteristics
Authors: Litleskare Sigbjørn , Calogiuri Giovanna
Published: March 2023

Introduction

The COVID-19 pandemic has been linked to increased mental health issues and reduced well-being. Researchers also reports increased frequency of nature visits during the pandemic, proposing it may mitigate some of these negative consequences. Using the case of Norway, a country with ample access to nature and relatively low levels of pandemic-related restrictions, this study sought to (i) understand how the COVID-19 crisis impacted patterns of nature visits and specific nature-based activities, (ii) examine how these patterns varied among different population groups and levels of restrictions, and (iii) explore the motives and facilitators for increased frequency of nature visits.

Methods

The data were retrieved from a national cross-sectional survey conducted in June 2021, which was designed to assess participants (n = 1,005, age > 15 years) habits in relation to nature visits and outdoor recreation since the beginning of the COVID-19 crisis, and associated factors.

Results

The results showed that 32 % of participants increased their frequency of nature visits during the crisis, while 11 % experienced a decrease. Multivariate logistic regression revealed significant positive associations between increased frequency of nature visits and longer duration of lockdown restrictions (OR [95% CI] = 2.35 [1.28–4.29] and 4.92 [2.77–8.74] for a few weeks and several months of lockdown, respectively). Increased frequency of nature visits was also more likely among women, younger respondents, and individuals from high-income households. A Cochran's Q test showed that the most common motive for increased frequency of nature visits was “To be physically active” (74%). The most commonly reported facilitators were the possibility of using natural environments as an alternative to gyms and organized sports alongside having more time available (58 and 49%, respectively).

Conclusion

These findings suggest that nature visits provided important opportunities for physical activity during the COVID-19 crisis, but also that the mental health benefits of nature visits during such times may be under-communicated. This highlights the importance of access to natural environments to promote physical activity and health, but also suggests that campaigns that specifically communicate the beneficial effects of nature visits during lockdowns or similar stressful situations might help people cope with the situation.
Accompanying your children: Living without parents at different stages of pre-adulthood and individual physical and mental health in adulthood
Authors: Jiang Yao , Xiao Hanling , Yang Fan
Published: March 2023

Objectives

This study examined how living without parents at different stages of childhood and adolescence affects physical and mental health in adulthood.

Methods

The data came from 3,464 survey respondents aged 18–36 in the 2018 China Labor-Force Dynamics Survey. Physical health was self-rated. Mental health was measured by the Center for Epidemiological Studies Depression scale. The ordered probit and ordinary least-squares regression analyses were employed to determine the associations between growing up without parents at different stages in pre-adulthood and individual physical and mental health in adulthood.

Results

Individuals who did not live with their parents during their minor years were more likely to report worse physical and mental health in adulthood, compared to those who lived with their parents. This difference was heterogeneous among different age stages and genders.

Conclusions

Absence of parents in the household has long-term impacts on the physical and mental health of children in adulthood, especially for females. The government should make feasible institutional arrangements to avoid the separation of minor children from their parents.
Disentangling the responses of dissolved organic carbon and nitrogen concentrations to overlapping drivers in a northeastern United States forested watershed
Authors: Ruckhaus Manya , Seybold Erin C. , Underwood Kristen L. , Stewart Bryn , Kincaid Dustin W. , Shanley James B. , Li Li , Perdrial Julia N.
Published: March 2023

The concurrent reduction in acid deposition and increase in precipitation impact stream solute dynamics in complex ways that make predictions of future water quality difficult. To understand how changes in acid deposition and precipitation have influenced dissolved organic carbon (DOC) and nitrogen (N) loading to streams, we investigated trends from 1991 to 2018 in stream concentrations (DOC, ~3,800 measurements), dissolved organic nitrogen (DON, ~1,160 measurements), and dissolved inorganic N (DIN, ~2,130 measurements) in a forested watershed in Vermont, USA. Our analysis included concentration-discharge (C-Q) relationships and Seasonal Mann-Kendall tests on long-term, flow-adjusted concentrations. To understand whether hydrologic flushing and changes in acid deposition influenced long-term patterns by liberating DOC and dissolved N from watershed soils, we measured their concentrations in the leachate of 108 topsoil cores of 5 cm diameter that we flushed with solutions simulating high and low acid deposition during four different seasons. Our results indicate that DOC and DON often co-varied in both the long-term stream dataset and the soil core experiment. Additionally, leachate from winter soil cores produced especially high concentrations of all three solutes. This seasonal signal was consistent with C-Q relation showing that organic materials (e.g., DOC and DON), which accumulate during winter, are flushed into streams during spring snowmelt. Acid deposition had opposite effects on DOC and DON compared to DIN in the soil core experiment. Low acid deposition solutions, which mimic present day precipitation, produced the highest DOC and DON leachate concentrations. Conversely, high acid deposition solutions generally produced the highest DIN leachate concentrations. These results are consistent with the increasing trend in stream DOC concentrations and generally decreasing trend in stream DIN we observed in the long-term data. These results suggest that the impact of acid deposition on the liberation of soil carbon (C) and N differed for DOC and DON vs. DIN, and these impacts were reflected in long-term stream chemistry patterns. As watersheds continue to recover from acid deposition, stream C:N ratios will likely continue to increase, with important consequences for stream metabolism and biogeochemical processes.
Environmental isotopes (δ18O–δ2H, 222Rn) and electrical conductivity in backtracking sources of urban pipe water, monitoring the stability of water quality and estimating pipe water residence time
Authors: Kebede Seifu , Hailu Kidist , Siraj Abdulhafiz , Birhanu Behailu
Published: March 2023

This study demonstrates the use of environmental tracers (Water isotopes-δ18O–δ2H, Radon-222Rn, and Electrical Conductivity-EC) as complementary tools for backtracking the water source, estimating pipe water residence time, and monitoring the instability of the water quality. Using the capital of Ethiopia, Addis Ababa, as a case study site, we demonstrate that water isotopes (δ18O andδ2H) effectively backtrack the tap water to its source (springs, reservoirs, shallow aquifers, or deep aquifers). 222Rn is shown to be effective for discriminating groundwater-sourced pipe networks from those that are dominated by surface waters. Our reconnaissance survey reveals that 222Rn, a tracer previously not considered to determine the pipe water residence time, can be used effectively to determine pipe water residence time in groundwater-sourced pipe networks. We recommend further research to explore the capability of 222Rn as a robust indicator of the pipe water residence time in an urban piped water network. The tracers reveal that 50% of the city obtains its water from groundwater and that the groundwater-sourced areas of the city show the highest water quality instability. The water quality in groundwater-sourced pipes varies depending on pumping stoppage owing to power interruptions. Surface water-sourced pipe water shows seasonal variations in water quality, with occasional large deviations from the normal trends following flow interruptions.
A call for strategic water-quality monitoring to advance assessment and prediction of wildfire impacts on water supplies
Authors: Murphy Sheila F. , Alpers Charles N. , Anderson Chauncey W. , Banta J. Ryan , Blake Johanna M. , Carpenter Kurt D. , Clark Gregory D. , Clow David W. , Hempel Laura A. , Martin Deborah A. , Meador Michael R. , Mendez Gregory O. , Mueller-Solger Anke B. , Stewart Marc A. , Payne Sean E. , Peterman Cara L. , Ebel Brian A.
Published: March 2023

Wildfires pose a risk to water supplies in the western U.S. and many other parts of the world, due to the potential for degradation of water quality. However, a lack of adequate data hinders prediction and assessment of post-wildfire impacts and recovery. The dearth of such data is related to lack of funding for monitoring extreme events and the challenge of measuring the outsized hydrologic and erosive response after wildfire. Assessment and prediction of post-wildfire surface water quality would be strengthened by the strategic monitoring of key parameters, and the selection of sampling locations based on the following criteria: (1) streamgage with pre-wildfire data; (2) ability to install equipment that can measure water quality at high temporal resolution, with a focus on storm sampling; (3) minimum of 10% drainage area burned at moderate to high severity; (4) lack of major water management; (5) high-frequency precipitation; and (6) availability of pre-wildfire water-quality data and (or) water-quality data from a comparable unburned basin. Water-quality data focused on parameters that are critical to human and (or) ecosystem health, relevant to water-treatment processes and drinking-water quality, and (or) inform the role of precipitation and discharge on flow paths and water quality are most useful. We discuss strategic post-wildfire water-quality monitoring and identify opportunities for advancing assessment and prediction. Improved estimates of the magnitude, timing, and duration of post-wildfire effects on water quality would aid the water resources community prepare for and mitigate against impacts to water supplies.
Assessing the effectiveness of environmental sampling for surveillance of foot-and-mouth disease virus in a cattle herd
Authors: Ellis John , Brown Emma , Colenutt Claire , Gubbins Simon
Published: March 2023

The survival of foot-and-mouth disease virus (FMDV) in the environment provides an opportunity for indirect transmission, both within and between farms. However it also presents the possibility of surveillance and detection via environmental sampling. This study assesses the effectiveness of environmental sampling strategies in the event of an outbreak, using a previous model for transmission of FMDV in a cattle herd that had been parameterized using data from transmission experiments and outbreaks. We show that environmental sampling can be an effective means of detecting FMDV in a herd, but it requires multiple samples to be taken on multiple occasions. In addition, environmental sampling can potentially detect FMDV in a herd more quickly than clinical inspection. For example, taking 10 samples every 3 days results in a mean time to detection of 6 days, which is lower than the mean time to detection estimated for the 2001 UK epidemic (8 days). We also show how environmental sampling could be used in a herd considered to be at risk as an alternative to pre-emptive culling. However, because of the time taken for virus to accumulate at the start of an outbreak, a reasonable level of confidence (> 99%) that an at-risk herd is indeed free from infection is unlikely to be achieved in less than 1 week.
The effect of C–C motif chemokine ligand 2 supplementation on in vitro maturation of porcine cumulus-oocyte complexes and subsequent developmental competence after parthenogenetic activation
Authors: Kim Sohee , Oh Dongjin , Choi Hyerin , Kim Mirae , Cai Lian , Jawad Ali , Haomiao Zheng , Lee Joohyeong , Kim Eunhye , Hyun Sang-Hwan
Published: March 2023

Porcine embryos are used for a variety of applications. However, the maturation rate in vitro remains low, and novel in vitro maturation (IVM) techniques that facilitate the collection of mature oocytes are necessary. C-C motif chemokine ligand 2 (CCL2) is a key periovulatory chemokine present in cumulus-oocyte complexes (COCs). We aimed to examine the effects of CCL2 supplementation during IVM on oocyte maturation and embryonic development. The CCL2 concentration was significantly higher in porcine follicular fluid (pFF) derived from follicles >8 mm in size than in pFF derived from smaller follicles. There was a significant increase in CCL2 mRNA levels in all follicular cells after IVM compared with that before IVM. We analyzed the localization of CCL2 and its receptor, the CCL2 receptor, in follicular cells. During IVM, different concentrations of CCL2 were added to COCs cultured in a maturation medium. After IVM, the group treated with 100 ng/mL CCL2 showed significantly higher metaphase II rates than the control group. All CCL2-treatment groups showed a significant increase in intracellular glutathione levels and a significant decrease in reactive oxygen species levels, compared to the control. In CCs treated with 100 ng/mL CCL2, the mRNA levels of BAX, CASP3, and NPR2 were significantly decreased. Furthermore, the mRNA levels of SOD1, SOD2, and CD44 were significantly increased. In oocytes treated with 10 ng/mL CCL2, mRNA levels of BAX and CASP3 were significantly decreased, whereas, NRF2 and NPM2 were significantly increased. ERK1 exhibited significantly increased mRNA expression in both CCs and oocytes treated with 10 ng/mL CCL2. The protein expression ratio of phosphorylated ERK1/2 to total ERK1/2 was significantly increased in CCs treated with 10 ng/mL CCL2. After parthenogenetic activation, cleavage rates were significantly improved in the 100 ng/mL CCL2 treatment group, and blastocyst formation rates were significantly enhanced in the 10 ng/mL CCL2 treatment group. Overall, our results suggest that IVM medium along with CCL2 improves porcine oocyte maturation and the development of parthenogenetically-activated embryos.
Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos
Authors: Ahmed Ahmed Ezzat , Sindi Ramya Ahmad , Yousef Nasra Ahmed , Hussein Hassan A. , Badr Magdy R. , Syaad Khalid M. Al , Al-Saeed Fatimah A. , Hassaneen Ahmed Saad A. , Abdelrahman Mohamed , Ali Montaser Elsayed
Published: March 2023

The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the in vitro maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities.