bluuebunny/crossref_metadata_2025 huggingface dataset:
What is the effect of supervised rehabilitation regime vs. self-management instruction following unicompartmental knee arthroplasty? – a pilot study in two cohorts
Authors: Omari Adam , Ingelsrud Lina Holm , Bandholm Thomas Quaade , Lentz Susanne Irene , Troelsen Anders , Gromov Kirill
Published: June 2021

Purpose

The optimal rehabilitation strategy after a unicompartmental knee arthroplasty (UKA) is unclear. This study aims to compare the effect of transitioning from a supervised to a self-management rehabilitation regime by pilot study of patient outcomes subsequent to UKA surgery.

Methods

Fifty consecutive patients scheduled to undergo unilateral UKA surgery at our institution between 22nd February 2016 and 18thof January 2017 were prospectively identified via local medical database and included. Performed UKAs were grouped into two cohorts, Supervised Cohort and Self-management Cohort, temporally separated by introduction of new rehabilitation. Self-management Cohort(n = 25) received an extensive inpatient rehabilitation regime along with outpatient referral to rehabilitation center. The Self-management Cohort(n = 25) were only instructed in use of crutches and free ambulation at own accord. Follow-up (F/U) was 1 year from receiving UKA. A range of outcomes were recorded, and between-cohort differences compared: knee joint range of motion, pain and functional limitations, length of stay (LOS), readmission rate, pain during activity and rest, and knee circumference.

Results

Complete data was obtained for n = 45 patients. The mean between-cohort difference in ROM (range of motion) from preoperatively to discharge was 15.4 degrees (CI:5.2,25.8, p = 0.004), favoring the supervised regime, with no difference detected in any outcome at 3- or 12 months F/U. Median LOS was 1 day in both cohorts.

Conclusion

Transition to a simple rehabilitation regime following UKA surgery was associated with decreased ROM at discharge, which was not present at 3-month F/U. We found no other between-cohort differences for any other outcomes at 3- and 12-month F/U including functional limitations, although the study was likely underpowered for these outcomes. We encourage large-scale replication of these findings using randomized designs.

Level of evidence

Therapeutic level II
Multilevel analysis of site, implant, and patient-level factors with peri-implant bleeding on probing: a cross sectional study
Authors: Nettemu Sunil Kumar , Nettem Sowmya , Singh Vijendra Pal , William Sheila Shirley , Gunasekaran Shargunan Selvanthan , Krisnan Malathi , Abas Adinegara Lutfi
Published: June 2021

Aim

This study was to evaluate the association between peri-implant bleeding on probing in peri-implant diseases and its association with multilevel factors (site specific factors, implant factors, and patient level factors).

Methodology

A cross-sectional study involved consented adult patients with ≥ 1 dental implant. Two calibrated operators examined the patients. BoP was outcome variable and peri-implant gingival biotype was principal predictor variable. The effects of site, implant, and patient level factors on BoP were assessed using a multilevel logistic regression model.

Results

Eighty patients for a total of 119 implants and 714 sites were included in the study. Bleeding on probing was observed in 42 implants (35.29%) with a significant higher risk observed in presence of gingival recession, thin peri-implant gingival biotype, duration of implant placement, smokers, and male patients.

Conclusion

Peri-implant bleeding on probing was associated with site specific, implant, and patient level factors.
Correction to: Infuence of herbicides under biofertilizer application on fennel (Foeniculum vulgare) yield and quality with special reference to herbicide residues
Authors: Abdallah Ibrahim , Amer Alia , El-Hefny Dalia
Published: June 2021

An amendment to this paper has been published and can be accessed via the original article.
Long non-coding RNA KCNQ1OT1 promotes cell viability and migration as well as inhibiting degradation of CHON-001 cells by regulating miR-126-5p/TRPS1 axis
Authors: Wang Binfeng , Liu Xiangwei
Published: June 2021

Background

Osteoarthritis (OA) is defined as a degenerative disease. Pivotal roles of long non-coding RNA (lncRNAs) in OA are widely elucidated. Herein, we intend to explore the function and molecular mechanism of lncRNA KCNQ1OT1 in CHON-001 cells.

Methods

Relative expression of KCNQ1OT1, miR-126-5p and TRPS1 was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability was examined by MTT assay. The migratory ability of chondrocytes was assessed by transwell assay. Western blot was used to determine relative protein expression of collagen II, MMP13 and TRPS1. Dual-luciferase reporter (DLR) assay was applied to test the target of lncRNA KCNQ1OT1 or miR-126-5p.

Results

Relative expression of KCNQ1OT1 and TRPS1 was reduced, whereas miR-126-5p was augmented in cartilage tissues of post-traumatic OA patients compared to those of subjects without post-traumatic OA. Increased KCNQ1OT1 or decreased miR-126-5p enhanced cell viability and migration, and repressed extracellular matrix (ECM) degradation in CHON-001 cells. MiR-126-5p was the downstream target of KCNQ1OT1, and it could directly target TRPS1. There was an inverse correlation between KCNQ1OT1 and miR-126-5p or between miR-126-5p and TRPS1. Meantime, there was a positive correlation between KCNQ1OT1 and TRPS1. The promoting impacts of KCNQ1OT1 on cell viability and migration as well as the suppressive impact of KCNQ1OT1 on ECM degradation were partially abolished by miR-126-5p overexpression or TRPS1 knockdown in CHON-001 cells.

Conclusions

Overexpression of KCNQ1OT1 attenuates the development of OA by sponging miR-126-5p to target TRPS1. Our findings may provide a possible therapeutic strategy for human OA in clinic.
Rheumatologist perspective of the Brazilian consensus for detection of auto antibodies in HEp-2 CELLS
Authors: Francescantonio Isadora Carvalho Medeiros , dos Santos Leandro Augusto Rodrigues , Francescantonio Paulo Luiz Carvalho , Andrade Luiz Eduardo Coelho , de Melo Cruvinel Wilson
Published: June 2021

Objective

To evaluate the perception of rheumatologists regarding the recommendations of the Brazilian Consensus for detection of Autoantibodies (BCA) on HEp-2 Cells by Indirect Immunofluorescence assay (IFA) and how BCA recommendations help in clinical practice.

Methodology

A structured questionnaire regarding the BCA recommendations for detection and interpretations of autoantibodies in HEp-2 cells was applied to randomly selected rheumatologists. The results were tabulated using the Microsoft® Excel program, expressed as a simple percentage and the dichotomous data were analyzed using the Chi-square test and the Epi Info® program.

Results

Four hundred fuorteen rheumatologists participated in the study: 70% of them considered their knowledge of the HEp-2 IFA test satisfactory or excellent, and 43% said they knew the BCA recommendations in general, without distinguishing the edition of the BCA to which they refer. The Revista Brasileira de Rheumatologia/Advances in Rheumatology was the means of dissemination most consulted by specialists (50%). According to the rheumatologists’ opinion, the most relevant pattern was the homogeneous nuclear (78%) and 65% stated they were satisfied with the BCA recommendations at a level of satisfaction greater than or equal to 80%. There was no significant difference in the perception of rheumatologists from the several Brazilian geographic regions.

Conclusion

Brazilian rheumatologists are aware of the BCA guidelines and most are satisfied with the content published, considering that the BCA recommendations assist positively in the clinical practice. Most rheumatologists recognize the patterns associated with rheumatic autoimmune diseases and have used BCA recommendations to interpret the results of the HEp-2 IFA test.
Burnout and coping among healthcare providers working in Saudi Arabia during the COVID-19 pandemic
Authors: AlJhani Sumayah , AlHarbi Hatim , AlJameli Shahad , Hameed Lama , AlAql Khozama , Alsulaimi Mohammed
Published: June 2021

Background

Burnout is defined as a syndrome resulting from chronic workplace stress that has not been successfully managed. It is characterised by feelings of energy depletion or exhaustion, increased mental distance from one’s job and reduced professional efficacy. The COVID-19 pandemic has created unexpected demands on healthcare systems worldwide and they have experienced numerous stressors. As the coping is one of the stressors management strategies that may affect burnout, this is a descriptive cross-sectional study aimed to estimate the frequency and level of burnout and its association with coping strategies among physicians and nurses in Saudi Arabia during the COVID-19 Pandemic using Copenhagen Burnout Inventory and Brief-COPE.

Results

Overall, 403 healthcare providers were recruited (85 physicians, 318 nurses). Personal, work-related and client-related burnout were detected among 67.5%, 68% and 58.3% of the respondents, respectively. The mean score for adaptive coping was (27.6 ± 10.3, median: 29 IQR: 14.0) out of 48, and the mean score for maladaptive coping was (14.2 ± 6.81, median: 14 IQR: 8.0) out of 36. Some factors associated with burnout were participants’ age group, professional position, number of family members and years of experience in the medical field. The personal, work-related and client-related burnout had inverse correlations with the overall adaptive coping category.

Conclusion

The frequency of burnout during the COVID-19 pandemic, particularly among nurses, was significant. Burnout was also frequent among both the younger age group and those with fewer years of experience. Some predictors were identified as having a close person infected with COVID-19, being assigned to treat COVID-19 patients, longer working hours, having sleeping hours affected by the pandemic and experiencing verbal or physical abuse from patients. In addition to a significant correlation between the adaptive coping category and the three burnout dimensions.
The expression of microRNA-331-3p and microRNA-23b3 in Egyptian patients with early-stage hepatocellular carcinoma in hepatitis C-related liver cirrhosis
Authors: Aboelwafa Reham A. , Ellakany Walid Ismail , Gamaleldin Marwa A. , Saad Marwa A.
Published: June 2021

Background

Hepatocellular carcinoma and hepatitis C are strongly associated. The current work aimed to study the expression levels of microRNA-331-3p and microRNA-23b-3p as propable biomarkers for detecting liver cancer (HCC) at its early stages in patients with HCV-related liver cirrhosis. The current prospective study included two hundred participants, divided into three groups: group I, 100 patients with HCV-related liver cirrhosis; group II, 50 HCC patients at early stages; and group III, 50 apparentlyhealthy controls. All patients had routine laboratory workup and ultrasound hepatic assessment. Values of microRNA-331-3p and microRNA-23b-3p were measured by real-time quantitative PCR.

Results

Levels of miR-331-3p were significantly higher in HCC patients than in cirrhotic patients and controls (p < 0.001), while levels of miR-23b-3p were significantly lower in HCC patients compared to cirrhotics and controls (p < 0.001). ROC curve revealed that miR-23b-3p had 80% sensitivity and 74% specificity, miR-331-3p had 66% sensitivity and 61% specificity, and AFP had 64% sensitivity and 61% specificity of 61% in discrimination between HCC patients from controls.

Conclusion

Serum miR-23b-3p is a more effective predictor than miR-331-3p and AFP for the development of hepatocellular carcinoma in hepatitis C (HCV)-related cirrhotic patients.
The impacts of interaction of audit litigation and ownership structure on audit quality
Authors: AL Abdullah Riyadh Jassim , AL Ani Mawih Kareem
Published: June 2021

This study examines the impact of the interactions of audit litigation and ownership structure on audit quality by Big 4 and non-Big 4 audit firms in Oman. This study uses modified audit opinion as proxies for audit quality, binary variable for audit litigation and percentage of shares owned by large shareholders and minority shareholders (consisting of Arab [non-GCC] shareholders) for ownership structure. The study uses size, risk, types of activity and ages of the firms as control variables. For the analysis and explanation of results descriptive statistics, correlation, regression techniques and T-test are used. Based on a sample of 107 listed companies on Muscat Securities Market (MSM) for 2013–2017, we find that audit litigation has a significant impact on audit quality for Big 4 audit firms, but not for non-Big 4 audit firms. Also, the results indicate that there is no difference between Big 4 and non-Big 4 audit firms as far as litigation risk is concerned.
Aerodynamic characteristics of a square cylinder with corner fins
Authors: Wang Qiulei , Jiang Qi , Hu Gang , Chen Xiao , Li Chao , Xiao Yiqing
Published: June 2021

This paper investigates the effect of fitting fins at the corners of a square cylinder on aerodynamic characteristics of the cylinder via wind tunnel tests and large eddy simulations (LES). Although it has been recognized that the corner fins have a remarkable effect on aerodynamic characteristics of a square cylinder, no study has been carried out to systematically evaluate this effect and reveal the underlying mechanism. Three types of corner fin configurations, i.e. fins fitted only to the leading corners, fins fitted only to the trailing corners, and fins fitted to both leading and trailing corners were studied. It was found that the corner fins significantly influence aerodynamic characteristics, such as mean drag coefficient, fluctuating lift coefficient, and vortex shedding of the cylinder. The influences of these corner fin configurations are very different. In general, the leading and trailing fins have an opposite effect on these characteristics. The mechanisms underlying these effects were clarified based on the flow regime visualized via LES. The interesting findings have practical significances not only for reducing aerodynamic forces and wind-induced vibration of infrastructures, but also for enhancing wind-induced vibration-based energy harvesting.
G-protein coupled receptor, PI3K and Rho signaling pathways regulate the cascades of Tau and amyloid-β in Alzheimer’s disease
Authors: Desale Smita Eknath , Chidambaram Hariharakrishnan , Chinnathambi Subashchandrabose
Published: June 2021

Alzheimer’s disease is a progressive neurodegenerative disease characterized by the presence of amyloid-β plaques in the extracellular environment and aggregates of Tau protein that forms neurofibrillary tangles (NFTs) in neuronal cells. Along with these pathological proteins, the disease shows neuroinflammation, neuronal death, impairment in the immune function of microglia and synaptic loss, which are mediated by several important signaling pathways. The PI3K/Akt-mediated survival-signaling pathway is activated by many receptors such as G-protein coupled receptors (GPCRs), triggering receptor expressed on myeloid cells 2 (TREM2), and lysophosphatidic acid (LPA) receptor. The signaling pathway not only increases the survival of neurons but also regulates inflammation, phagocytosis, cellular protection, Tau phosphorylation and Aβ secretion as well. In this review, we focused on receptors, which activate PI3K/Akt pathway and its potential to treat Alzheimer’s disease. Among several membrane receptors, GPCRs are the major drug targets for therapy, and GPCR signaling pathways are altered during Alzheimer’s disease. Several GPCRs are involved in the pathogenic progression, phosphorylation of Tau protein by activation of various cellular kinases and are involved in the amyloidogenic pathway of amyloid-β synthesis. Apart from various GPCR signaling pathways, GPCR regulating/ interacting proteins are involved in the pathogenesis of Alzheimer’s disease. These include several small GTPases, Ras homolog enriched in brain, GPCR associated sorting proteins, β-arrestins, etc., that play a critical role in disease progression and has been elaborated in this review.
Morbid Vitalism
Authors: Clarke Tim
Published: June 2021

This essay frames Djuna Barnes’s 1936 novel Nightwood as an attempt to overcome an impasse between the discourses of hope and the discourses of despair in an interwar period in many ways preoccupied with questions of mortality. Synthesizing Decadent aesthetics and elements of Spinoza’s vitalist philosophy, Barnes produces a “morbid vitalism,” exemplified by Dr. Matthew O’Connor, by which life and death are conceived as variant expressions of a single force, and the subject is modeled as an assemblage of affects, impersonal but inherently social, that can be understood primarily through its pursuit of what Jack Halberstam has called “generative models of failure.” In exploring this mode of subjectivity, Barnes seeks to undermine a host of ostensible oppositions (hope and fear, ascendence and decadence, success and failure, morbidity and vitality), opening up a conceptual and affective space for thinking through—if not necessarily beyond—the ubiquity of despair in twentieth-century modernity. Ultimately, morbid vitalism points a way toward a broader conversation between life-oriented modernist scholarship on vitalism and affect, on the one hand, and ongoing inquiries into the relationship among death, Decadence, and modernism, on the other.
Where Is North?
Authors: Kim Sora
Published: June 2021

We take the cardinal directions for granted, but they are social constructs. Directionality is relative to how we locate central points, and these choices reflect a sense of direction in a society. This article illustrates how the notion of “center” changed in Korean society by comparing land registers of the Korean Empire (1897–1910) and the Japanese colonial period (1910–45). The colonial government prioritized mapping with scale, contours, and cardinal directions. As a result, the entire country was mapped to conform to a procrustean order. By contrast, there had been no cadastral map for centuries prior. Instead, the location of each parcel was described in textual information with four cardinal points. The author argues that fundamental difference between the two notions of “center” lay in the consciousness of the relationship between the human and the natural. The difference was expressed through the contrast in their respective conformity and flexibility, standardization and diversity.
Cerebral and tumoral blood flow in adult gliomas: a systematic review of results from magnetic resonance imaging
Authors: Waqar Mueez , Lewis Daniel , Agushi Erjon , Gittins Matthew , Jackson Alan , Coope David
Published: June 2021

Objective:

Blood flow is the rate of blood movement and relevant to numerous processes, though understudied in gliomas. The aim of this review was to pool blood flow metrics obtained from MRI modalities in adult supratentorial gliomas.

Methods:

MEDLINE, EMBASE and the Cochrane database were queried 01/01/2000–31/12/2019. Studies measuring blood flow in adult Grade II–IV supratentorial gliomas using dynamic susceptibility contrast (DSC) MRI, dynamic contrast enhanced MRI (DCE-MRI) or arterial spin labelling (ASL) were included. Absolute and relative cerebral blood flow (CBF), peritumoral blood flow and tumoral blood flow (TBF) were reported.

Results:

34 studies were included with 1415 patients and 1460 scans. The mean age was 52.4 ± 7.3 years. Most patients had glioblastoma (n = 880, 64.6%). The most common imaging modality was ASL (n = 765, 52.4%) followed by DSC (n = 538, 36.8%). Most studies were performed pre-operatively (n = 1268, 86.8%). With increasing glioma grade (II vs IV), TBF increased (70.8 vs 145.5 ml/100 g/min, p < 0.001) and CBF decreased (85.3 vs 49.6 ml/100 g/min, p < 0.001). In Grade IV gliomas, following treatment, CBF increased in ipsilateral (24.9 ± 1.2 vs 26.1 ± 0.0 ml/100 g/min, p < 0.001) and contralateral white matter (25.6 ± 0.2 vs 26.0± 0.0 ml/100 g/min, p < 0.001).

Conclusion:

Our findings demonstrate that increased mass effect from high-grade gliomas impairs blood flow within the surrounding brain that can improve with surgery.

Advances in knowledge:

This systematic review demonstrates how mass effect from brain tumours impairs blood flow in the surrounding brain parenchyma that can improve with treatment.
Pharmacological management of seizures in patients with COVID-19: a systematic review
Authors: Mante Priscilla Kolibea , Adomako Nana Ofori , Omuojine John-Paul , Antwi Paulina
Published: June 2021

Background: Some patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been reported to exhibit neurological symptoms such as seizures and impaired consciousness. Our study reviews reported cases to assess the pharmacological approach to managing seizures in SARS-CoV-2 patients and associated outcomes.

Methods: A systematic review of case reports on the incidence of seizures following coronavirus disease 2019 (COVID-19) among patients that reported use of antiepileptic drugs (AEDs) in management was performed by using the PRISMA (preferred reporting items for systematic reviews and meta-analysis) guidelines. Databases used included EMBASE, PubMed, SCOPUS, and Google Scholar. Data was presented as qualitative and descriptive data.

Results: In total, 67 articles were selected for full-text assessment, of which 19 were included in the final review. Patients had a median age of 54 years, most of whom were male. Remdisivir, dexamethasone, Laminavir, hydroxychloroquine, azithromycin, and Lopinavir-ritonavir were common agents used in the management of COVID-19. Most patients presented with either generalized tonic-clonic seizures or status epilepticus. Most patients received levetiracetam as drug choice or as part of their regimen. Other AEDs commonly prescribed included midazolam and sodium valproate. Some patients received no antiepileptic drug therapy. Most of the patients who died had more than one comorbidity. Also, most of the patients who died received COVID-19 treatment drugs. None of the patients who received midazolam as drug choice or as part of their regimen developed recurrent seizures in contrast to patients who received levetiracetam and sodium valproate as drug choice or as part of their regimen. Interestingly, none of the patients who received no AEDs suffered recurrent seizures or died.

Conclusions: Standard guidelines for managing seizures in COVID-19 patients may be required. A limitation of this review is that it involved the use of case reports with no controls and a small number of patients.
Pan-cancer chromatin analysis of the human vtRNA genes uncovers their association with cancer biology
Authors: Fort Rafael Sebastián , Duhagon María Ana
Published: June 2021

Background: The vault RNAs (vtRNAs) are a class of 84-141-nt eukaryotic non-coding RNAs transcribed by RNA polymerase III, associated to the ribonucleoprotein complex known as vault particle. Of the four human vtRNA genes, vtRNA1-1, vtRNA1-2 and vtRNA1-3, clustered at locus 1, are integral components of the vault particle, while vtRNA2-1 is a more divergent homologue located in a second locus. Gene expression studies of vtRNAs in large cohorts have been hindered by their unsuccessful sequencing using conventional transcriptomic approaches.

Methods: VtRNA expression in The Cancer Genome Atlas (TCGA) Pan-Cancer cohort was estimated using the genome-wide DNA methylation and chromatin accessibility data (ATAC-seq) of their genes as surrogate variables. The association between vtRNA expression and patient clinical outcome, immune subtypes and transcriptionally co-regulated gene programs was analyzed in the dataset.

Results: VtRNAs promoters are enriched in transcription factors related to viral infection. VtRNA2-1 is likely the most independently regulated homologue. VtRNA1-1 has the most accessible chromatin, followed by vtRNA1-2, vtRNA2-1 and vtRNA1-3. VtRNA1-1 and vtRNA1-3 chromatin status does not significantly change in cancer tissues. Meanwhile, vtRNA2-1 and vtRNA1-2 expression is widely deregulated in neoplastic tissues and its alteration is compatible with a broad oncogenic role for vtRNA1-2, and both tumor suppressor and oncogenic functions for vtRNA2-1. Yet, vtRNA1-1, vtRNA1-2 and vtRNA2-1 promoter DNA methylation predicts a shorter patient overall survival cancer-wide. In addition, gene ontology analyses of vtRNAs co-regulated genes identify a chromosome regulatory domain, epithelial differentiation, immune and thyroid cancer gene sets for specific vtRNAs. Furthermore, vtRNA expression patterns are associated with cancer immune subtypes and vtRNA1-2 expression is positively associated with cell proliferation and wound healing.

Conclusions: Our study presents the landscape of vtRNA chromatin status cancer-wide, identifying co-regulated gene networks and ontological pathways associated with the different vtRNA genes that may account for their diverse roles in cancer.
The impact of condom use on the HIV epidemic
Authors: Stover John , Teng Yu
Published: June 2021

Background: Condom promotion and supply was one the earliest interventions to be mobilized to address the HIV pandemic. Condoms are inexpensive and provide protection against transmission of HIV and other sexually transmitted diseases (STIs) as well as against unintended pregnancy. As many as 16 billion condoms may be used annually in all low- and middle-income countries (LMIC). In recent years the focus of HIV programs as been on testing and treatment and new technologies such as PrEP. Rates of condom use have stopped increasing short of UNAIDS targets and funding from donors is declining.

Methods: We applied a mathematical HIV transmission model to 77 high HIV burden countries to estimate the number of HIV infections that would have occurred from 1990 to 2019 if condom use had remained at 1990 levels.

Results: The results suggest that current levels of HIV would be five times higher without condom use and that the scale-up in condoms use averted about 117 million HIV infections.

Conclusions: HIV programs should ensure that affordable condoms are consistently available and that the benefits of condom use are widely understood.
MANFAAT AKUNTANSI BASIS AKRUAL: STUDI KASUS PADA ENTITAS PEMERINTAHAN PROVINSI DAERAH ISTIMEWA YOGYAKARTA, INDONESIA
Authors: Tasri Ansharullah
Published: June 2021

Pelaporan berbasis akrual bermanfaat dalam mengevaluasi kinerja pemerintah terkait biaya jasa layanan, efisiensi, dan pencapaian tujuan. Dengan pelaporan berbasis akrual, posisi keuangan pemerintah daerah dan perubahannya serta bagaimana pemerintah daerah mendanai kegiatannya dapat diidentifikasi dan diukur secara tepat. Penelitian ini bertujuan untuk mengeksplorasi manfaat akuntansi akrual. Terdapat lima indikator yang digunakan untuk menilai tingkat manfaat yaitu penilaian kinerja, pelayanan publik, kebutuhan arus kas, alokasi sumber daya, dan kewajiban akuntabilitas. Masih sedikit literatur yang menjelaskan tentang manfaat informasi akrual di Pemerintah Indonesia. Hal itu dikarenakan kewajiban pelaksanaan akuntansi akrual baru berlaku untuk periode pelaporan 2015. Oleh karena itu penelitian ini bertujuan mengeksplorasi manfaat akuntansi akrual pada internal pemerintah. Selain itu, tujuan penelitian ini juga untuk mengetahui manfaat akun-akun akrual terhadap kinerja pemerintah. Responden yang digunakan pada penelitian ini sejumlah 80 orang yaitu sekretaris dan kepala sub bagian keuangan yang ada pada 40 SKPD/OPD pada Pemerintah Daerah Daerah Istimewa Yogyakarta. Penelitian dilakukan dengan memberikan kuesioner yang berisi pertanyaan dengan jawaban skala likert 1-5 atau pada kategori paling rendah sangat tidak bermanfaat dan pada kategori paling tinggi sangat bermanfaat. Penelitian ini menggunakan analisis statistik deskriptif. Hasil penelitian menunjukkan bahwa akuntansi akrual bermanfaat berdasarkan penilaian terhadap lima indikator yang terdapat pada penelitian ini.
Making a business case in healthcare: the value of careful consideration
Authors: Henriksson Anna Maria , Walker Matthew , Thakrar Sonali V
Published: June 2021

A well thought-out business case offers an opportunity for clinicians to bring about evidenced-based change in clinical practice. It is a powerful tool that can have a significant impact on healthcare services and patient outcomes, while also offering desirable solutions to the NHS, which is facing growing healthcare demands on increasingly limited resources. The role of a business case is to justify the need for change, argue its value, gain support from leadership and illustrate how it can be sustainably implemented.

This article guides clinicians through the process of producing a successful business case using the five-case model, which can be applied to the majority of improvement projects or services in healthcare settings. Clinicians might use this guide to support the development of a case to make a change in their own workplace or to help them take a full part in wider system changes.
Performing horizontal to vertical ratio testing in stiff soils in and around Grand Teton National Park
Authors: Griffiths Shawn , Ansariaval Abbas
Published: June 2021

Horizontal to vertical spectral ratio (HVSR) testing was completed at two cross sections in and around GTNP. The HVSR testing produced reliable estimates of the fundamental frequencies for many of the sites tested. The goal of the testing was to determine a depth of soil above competent bedrock. However the fundamental frequencies recorded yielded predicted depths that are much shallower than expected. Also the predicted depths did not increase at greater distance from the Teton Range, which would be expected at these sites. Based on these predictions the authors do not believe the frequencies recorded are a good indication of the depth of the soil above bedrock but instead it is believed that the depths correspond with a layer of softer topsoil/overburden above a stiffer gravel layer. Although the goal of measuring the depth of soil above bedrock was not met, HVSR produced results that may be useful to others for determination of a fundamental frequency of resonance at our testing locations.

 

Featured photo by Anna Cressman, taken from the AMK Ranch photo collection. https://flic.kr/p/2jjWZGT
Wave-optics simulation of dynamic speckle: II. In an image plane
Authors: Burrell Derek J. , Spencer Mark F. , Van Zandt Noah R. , Driggers Ronald G.
Published: June 2021

This two-part paper demonstrates the use of wave-optics simulations to model the effects of dynamic speckle. In Part II, we formulate closed-form expressions for the analytical irradiance correlation coefficient, specifically in the image plane of an optical system. These expressions are for square, circular, and Gaussian limiting apertures and four different modes of extended-object motion, including in-plane and out-of-plane translation and rotation. Using a phase-screen approach, we then simulate the equivalent scattering from an optically rough extended object, where we assume that the surface heights are uniformly distributed and delta correlated from grid point to grid point. For comparison to the analytical irradiance correlation coefficient, we also calculate the numerical irradiance correlation coefficient from the dynamic speckle after propagation from the simulated object plane to the simulated image plane. Overall, the analytical and numerical results definitely demonstrate that, relative to theory, the dynamic speckle in the simulated image plane is properly correlated from one frame to the next. Such validated wave-optics simulations provide the framework needed to model more sophisticated setups and obtain accurate results for system-level studies.
Measuring perceived sweetness by monitoring sorbitol concentration in apples using a non-destructive polarization-based readout
Authors: Sarykar Mukul , Assaad Maher
Published: June 2021

The determination and qualification of sugars in fruits are important for quality control and assurance of horticultural produce. The sugars determine the sweetness levels in fruits. The requirement for a universal technique that is also robust to predict the sweetness of the fruit in a non-destructive fashion is immense. The handheld refractometer, hydrometer, electronic tongues, and high-pressure liquid chromatography (HPLC) in combination with other detectors have long been used to determine the sweetness of horticultural produce. Though these techniques are very accurate and useful, they require extensive sample preparation and are generally time-consuming and expensive. Optical techniques like visible to near-infrared spectroscopy (vis/NIRS) are simple in use and can rapidly predict the sweetness of the fruit in a non-destructive fashion. The instrumentation used in these techniques is simple and cost-effective for routine analysis of the fruits. However, their systems need calibration for each sample, and the measurement variation depends on the type of horticultural produce on which measurements are done. An optical-based technique is proposed that uses reflected phase information of the incident light and correlates the same to the presence of sorbitol concentration in apples. The refractive index of sorbitol varies as the fruit ripens due to its change in concentration, and the reflected phase information accordingly changes. Monitoring the reflected phase information allows a prediction mechanism of the sweetness content in the fruit.
Fano resonance for applications
Authors: Limonov Mikhail F.
Published: June 2021

Fano resonance is a universal phenomenon observed in many areas where wave propagation and interference are possible. Fano resonance arises from the interference of broad and narrow spectra of radiation and becomes an important tool for many applications in the physical, chemical, and biological sciences. At the beginning of this paper, we consider Fano resonances in individual particles, primarily of spherical and cylindrical shapes, and discuss their connection with the physics of bound states in the continuum that determine the high quality factors of resonators. Further, we discuss two areas in which structures with Fano resonances have already found or will find real application in the nearest future—sensors and lasers. The penultimate section concerns our future, which will be associated with the complete replacement of electronic processing, transmission, and storage of information with optical devices as many hope. It is believed that this sophisticated goal can be achieved with devices that implement the slow-light regime associated with the phenomenon of electromagnetically induced transparency, which can be considered as a special case of Fano resonance. The review completes with one more promising topic related to quantum electrodynamics in structures with Fano cavities.
Modulation based ranging for direct displacement measurements of a dynamic surface
Authors: Rainey Kevin , Gilbertson Steve , Kalb Daniel , Beery Thomas
Published: June 2021

We developed a method for directly measuring displacement of a moving surface for use with dynamic or high explosive driven experiments. The technique, called “Modulation Based Ranging” (MBR), overcomes the errors associated with integrating a velocity history of an object undergoing non-radial flow, while also providing the exact displacement of the object with sub 100 µm resolution. A discussion of sources of phase sensitive errors is presented along with a demonstration of the applied corrections. Excellent agreement between MBR and integrated velocity from the Photonic Doppler Velocimetry (PDV) technique was observed when no non-radial flow was present. We then demonstrated the ability of MBR to accurately measure true displacement of a surface subjected to a strong non-radial component.
Sub-millisecond switching of multi-level liquid crystal on silicon spatial light modulators for increased information bandwidth
Authors: Pivnenko Mike , Li Kun , Chu Daping
Published: June 2021

Sub-millisecond response time with a refresh rate higher than 2000 frames per second (fps) and no degradation of the contrast ratio or diffraction efficiency is demonstrated in working liquid crystal on silicon (LCOS) spatial light modulators (SLMs) with 8-bit grey levels of amplitude and phase modulations. This makes possible to achieve an information bandwidth of about 190 Gb s-1 with a 4k LCOS operating at 10-bit phase modulation levels. The normalised contrast stays at almost the unit level for a frame rate up to 1700 fps and at higher than 0.9 for 2500 fps. The diffraction efficiency stays above -1.0 dB for a frame rate up to 2400 fps. Such a fast response allows us to eliminate image blurring in replaying a fast movie.
Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy
Authors: Wells Chad R. , Cao Youfang , Durham David P. , Byrareddy Siddappa N. , Ansari Aftab A. , Ruddle Nancy H. , Townsend Jeffrey P. , Galvani Alison P. , Perelson Alan S.
Published: June 2021

Treating macaques with an anti-α4β7 antibody under the umbrella of combination antiretroviral therapy (cART) during early SIV infection can lead to viral remission, with viral loads maintained at < 50 SIV RNA copies/ml after removal of all treatment in a subset of animals. Depletion of CD8+lymphocytes in controllers resulted in transient recrudescence of viremia, suggesting that the combination of cART and anti-α4β7 antibody treatment led to a state where ongoing immune responses kept the virus undetectable in the absence of treatment. A previous mathematical model of HIV infection and cART incorporates immune effector cell responses and exhibits the property of two different viral load set-points. While the lower set-point could correspond to the attainment of long-term viral remission, attaining the higher set-point may be the result of viral rebound. Here we expand that model to include possible mechanisms of action of an anti-α4β7 antibody operating in these treated animals. We show that the model can fit the longitudinal viral load data from both IgG control and anti-α4β7 antibody treated macaques, suggesting explanations for the viral control associated with cART and an anti-α4β7 antibody treatment. This effective perturbation to the virus-host interaction can also explain observations in other nonhuman primate experiments in which cART and immunotherapy have led to post-treatment control or resetting of the viral load set-point. Interestingly, because the viral kinetics in the various treated animals differed—some animals exhibited large fluctuations in viral load after cART cessation—the model suggests that anti-α4β7 treatment could act by different primary mechanisms in different animals and still lead to post-treatment viral control. This outcome is nonetheless in accordance with a model with two stable viral load set-points, in which therapy can perturb the system from one set-point to a lower one through different biological mechanisms.
Mosquito-bite infection of humanized mice with chikungunya virus produces systemic disease with long-term effects
Authors: Hibl Brianne M. , Dailey Garnes Natalie J. M. , Kneubehl Alexander R. , Vogt Megan B. , Spencer Clinton Jennifer L. , Rico-Hesse Rebecca R.
Published: June 2021

Chikungunya virus (CHIKV) is an emerging, mosquito-borne alphavirus responsible for acute to chronic arthralgias and neuropathies. Although it originated in central Africa, recent reports of disease have come from many parts of the world, including the Americas. While limiting human CHIKV cases through mosquito control has been used, it has not been entirely successful. There are currently no licensed vaccines or treatments specific for CHIKV disease, thus more work is needed to develop effective countermeasures. Current animal research on CHIKV is often not representative of human disease. Most models use CHIKV needle inoculation via unnatural routes to create immediate viremia and localized clinical signs; these methods neglect the natural route of transmission (the mosquito vector bite) and the associated human immune response. Since mosquito saliva has been shown to have a profound effect on viral pathogenesis, we evaluated a novel model of infection that included the natural vector,Aedesspecies mosquitoes, transmitting CHIKV to mice containing components of the human immune system. Humanized mice infected by 3–6 mosquito bites showed signs of systemic infection, with demonstrable viremia (by qRT-PCR and immunofluorescent antibody assay), mild to moderate clinical signs (by observation, histology, and immunohistochemistry), and immune responses consistent with human infection (by flow cytometry and IgM ELISA). This model should give a better understanding of human CHIKV disease and allow for more realistic evaluations of mechanisms of pathogenesis, prophylaxis, and treatments.
Coronavirus surveillance in wildlife from two Congo basin countries detects RNA of multiple species circulating in bats and rodents
Authors: Kumakamba Charles , Niama Fabien R. , Muyembe Francisca , Mombouli Jean-Vivien , Kingebeni Placide Mbala , Nina Rock Aime , Lukusa Ipos Ngay , Bounga Gerard , N’Kawa Frida , Nkoua Cynthia Goma , Atibu Losoma Joseph , Mulembakani Prime , Makuwa Maria , Tamufe Ubald , Gillis Amethyst , LeBreton Matthew , Olson Sarah H. , Cameron Kenneth , Reed Patricia , Ondzie Alain , Tremeau-Bravard Alex , Smith Brett R. , Pante Jasmine , Schneider Bradley S. , McIver David J. , Ayukekbong James A. , Hoff Nicole A. , Rimoin Anne W. , Laudisoit Anne , Monagin Corina , Goldstein Tracey , Joly Damien O. , Saylors Karen , Wolfe Nathan D. , Rubin Edward M. , Bagamboula MPassi Romain , Muyembe Tamfum Jean J. , Lange Christian E.
Published: June 2021

Coronaviruses play an important role as pathogens of humans and animals, and the emergence of epidemics like SARS, MERS and COVID-19 is closely linked to zoonotic transmission events primarily from wild animals. Bats have been found to be an important source of coronaviruses with some of them having the potential to infect humans, with other animals serving as intermediate or alternate hosts or reservoirs. Host diversity may be an important contributor to viral diversity and thus the potential for zoonotic events. To date, limited research has been done in Africa on this topic, in particular in the Congo Basin despite frequent contact between humans and wildlife in this region. We sampled and, using consensus coronavirus PCR-primers, tested 3,561 wild animals for coronavirus RNA. The focus was on bats (38%), rodents (38%), and primates (23%) that posed an elevated risk for contact with people, and we found coronavirus RNA in 121 animals, of which all but two were bats. Depending on the taxonomic family, bats were significantly more likely to be coronavirus RNA-positive when sampled either in the wet (Pteropodidae and Rhinolophidae) or dry season (Hipposideridae, Miniopteridae, Molossidae, and Vespertilionidae). The detected RNA sequences correspond to 15 alpha- and 6 betacoronaviruses, with some of them being very similar (>95% nucleotide identities) to known coronaviruses and others being more unique and potentially representing novel viruses. In seven of the bats, we detected RNA most closely related to sequences of the human common cold coronaviruses 229E or NL63 (>80% nucleotide identities). The findings highlight the potential for coronavirus spillover, especially in regions with a high diversity of bats and close human contact, and reinforces the need for ongoing surveillance.
Effects of substrate stiffness and actin velocity on in silico fibronectin fibril morphometry and mechanics
Authors: Weinberg Seth H. , Saini Navpreet , Lemmon Christopher A.
Published: June 2021

Assembly of the extracellular matrix protein fibronectin (FN) into insoluble, viscoelastic fibrils is a critical step during embryonic development and wound healing; misregulation of FN fibril assembly has been implicated in many diseases, including fibrotic diseases and cancer. We have previously developed a computational model of FN fibril assembly that recapitulates the morphometry and mechanics of cell-derived FN fibrils. Here we use this model to probe two important questions: how is FN fibril formation affected by the contractile phenotype of the cell, and how is FN fibril formation affected by the stiffness of the surrounding tissue? We show that FN fibril formation depends strongly on the contractile phenotype of the cell, but only weakly on in vitro substrate stiffness, which is an analog for in vivo tissue stiffness. These results are consistent with previous experimental data and provide a better insight into conditions that promote FN fibril assembly. We have also investigated two distinct phenotypes of FN fibrils that we have previously identified; we show that the ratio of the two phenotypes depends on both substrate stiffness and contractile phenotype, with intermediate contractility and high substrate stiffness creating an optimal condition for stably stretched fibrils. Finally, we have investigated how re-stretch of a fibril affects cellular response. We probed how the contractile phenotype of the re-stretching cell affects the mechanics of the fibril; results indicate that the number of myosin motors only weakly affects the cellular response, but increasing actin velocity results in a decrease in the apparent stiffness of the fibril and a decrease in the stably-applied force to the fibril. Taken together, these results give novel insights into the combinatorial effects of substrate stiffness and cell contractility on FN fibril assembly.
Identification and evaluation of the inhibitory effect of Prunella vulgaris extract on SARS-coronavirus 2 virus entry
Authors: Ao Zhujun , Chan Mable , Ouyang Maggie Jing , Olukitibi Titus Abiola , Mahmoudi Mona , Kobasa Darwyn , Yao Xiaojian
Published: June 2021

Until now, antiviral therapeutic agents are still urgently required for treatment or prevention of SARS-coronavirus 2 (SCoV-2) virus infection. In this study, we established a sensitive SCoV-2 Spike glycoprotein (SP), including an SP mutant D614G, pseudotyped HIV-1-based vector system and tested their ability to infect ACE2-expressing cells. Based on this system, we have demonstrated that an aqueous extract from the Natural herb Prunella vulgaris (NhPV) displayed potent inhibitory effects on SCoV-2 SP (including SPG614 mutant) pseudotyped virus (SCoV-2-SP-PVs) mediated infections. Moreover, we have compared NhPV with another compound, Suramin, for their anti-SARS-CoV-2 activities and the mode of their actions, and found that both NhPV and Suramin are able to directly interrupt SCoV-2–SP binding to its receptor ACE2 and block the viral entry step. Importantly, the inhibitory effects of NhPV and Suramin were confirmed by the wild type SARS-CoV-2 (hCoV-19/Canada/ON-VIDO-01/2020) virus infection in Vero cells. Furthermore, our results also demonstrated that the combination of NhPV/Suramin with an anti-SARS-CoV-2 neutralizing antibody mediated a more potent blocking effect against SCoV2-SP-PVs. Overall, by using SARS-CoV-2 SP-pseudotyped HIV-1-based entry system, we provide strong evidence that NhPV and Suramin have anti-SARS-CoV-2 activity and may be developed as a novel antiviral approach against SARS-CoV-2 infection.