bluuebunny/crossref_metadata_2025 huggingface dataset:
Expression of Enzymes Associated with Prostaglandin Synthesis in Equine Conceptuses
Authors: Budik Sven , Walter Ingrid , Leitner Marie-Christine , Ertl Reinhard , Aurich Christine
Published: April 2021

In the horse, mobility of the conceptus is required for maternal recognition of pregnancy depending on secretion of prostaglandins by the conceptus. The aim of this study was to determine the expression and localization of key enzymes of the different pathways leading to synthesis of prostaglandin E2 and F2α in the equine conceptus during the mobility phase. Enzyme expression was analyzed via quantitative RT-PCR in total RNA samples of equine conceptuses collected on days 10 (n = 5), 12 (n = 12), 14 (n = 5) and 16 (n = 7) from healthy mares. Relative abundance of cyclooxygenase (COX)-2 mRNA was higher (p < 0.05) than of COX-1 irrespective of conceptus age and for phospholipase A2 on day 16 in comparison to all other days (p < 0.01). Abundance of mRNA of cytosolic and microsomal prostaglandin E synthase (PGES) and of carbonyl reductase (CBR) 1 was not influenced by conceptus age. Immunohistochemically, COX-1, COX-2, as well as cytosolic and microsomal PGES were present in both the ectodermal and endodermal layer of the yolk sac wall. CBR-1 was restricted to periembryonic disc area. The localisation of the key enzymes explains the mechanism of embryo mobility. In vitro incubation of primary trophoblast cell cultures with oxytocin had no effect on key enzyme synthesis.
Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria
Authors: Galler Herbert , Luxner Josefa , Petternel Christian , Reinthaler Franz F. , Habib Juliana , Haas Doris , Kittinger Clemens , Pless Peter , Feierl Gebhard , Zarfel Gernot
Published: April 2021

In recent years, antibiotic-resistant bacteria with an impact on human health, such as extended spectrum β-lactamase (ESBL)-containing Enterobacteriaceae, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE), have become more common in food. This is due to the use of antibiotics in animal husbandry, which leads to the promotion of antibiotic resistance and thus also makes food a source of such resistant bacteria. Most studies dealing with this issue usually focus on the animals or processed food products to examine the antibiotic resistant bacteria. This study investigated the intestine as another main habitat besides the skin for multiresistant bacteria. For this purpose, faeces samples were taken directly from the intestines of swine (n = 71) and broiler (n = 100) during the slaughter process and analysed. All samples were from animals fed in Austria and slaughtered in Austrian slaughterhouses for food production. The samples were examined for the presence of ESBL-producing Enterobacteriaceae, MRSA, MRCoNS and VRE. The resistance genes of the isolated bacteria were detected and sequenced by PCR. Phenotypic ESBL-producing Escherichia coli could be isolated in 10% of broiler casings (10 out of 100) and 43.6% of swine casings (31 out of 71). In line with previous studies, the results of this study showed that CTX-M-1 was the dominant ESBL produced by E. coli from swine (n = 25, 83.3%) and SHV-12 from broilers (n = 13, 81.3%). Overall, the frequency of positive samples with multidrug-resistant bacteria was lower than in most comparable studies focusing on meat products.
The Human 2-Cys Peroxiredoxins form Widespread, Cysteine-Dependent- and Isoform-Specific Protein-Protein Interactions
Authors: van Dam Loes , Pagès-Gallego Marc , Polderman Paulien E. , van Es Robert M. , Burgering Boudewijn M. T. , Vos Harmjan R. , Dansen Tobias B.
Published: April 2021

Redox signaling is controlled by the reversible oxidation of cysteine thiols, a post-translational modification triggered by H2O2 acting as a second messenger. However, H2O2 actually reacts poorly with most cysteine thiols and it is not clear how H2O2 discriminates between cysteines to trigger appropriate signaling cascades in the presence of dedicated H2O2 scavengers like peroxiredoxins (PRDXs). It was recently suggested that peroxiredoxins act as peroxidases and facilitate H2O2-dependent oxidation of redox-regulated proteins via disulfide exchange reactions. It is unknown how the peroxiredoxin-based relay model achieves the selective substrate targeting required for adequate cellular signaling. Using a systematic mass-spectrometry-based approach to identify cysteine-dependent interactors of the five human 2-Cys peroxiredoxins, we show that all five human 2-Cys peroxiredoxins can form disulfide-dependent heterodimers with a large set of proteins. Each isoform displays a preference for a subset of disulfide-dependent binding partners, and we explore isoform-specific properties that might underlie this preference. We provide evidence that peroxiredoxin-based redox relays can proceed via two distinct molecular mechanisms. Altogether, our results support the theory that peroxiredoxins could play a role in providing not only reactivity but also selectivity in the transduction of peroxide signals to generate complex cellular signaling responses.
Special Issue on Pesticide Applications in Agricultural Systems
Authors: Manetto Giuseppe , Cerruto Emanuele , Longo Domenico , Papa Rita
Published: April 2021

The European Directive 2009/128/EC on the Sustainable Use of Pesticides recognizes the use of Plant Protection Products (PPP) as having great impact on human health and the environment [...]
Effect of Allium senescens Extract on Sorafenib Resistance in Hepatocarcinoma Cells
Authors: Park Sohyeon , Park Yoonjin , Shin Heejong , Kim Boyong , Lee Seunggwan
Published: April 2021

Although Allium species are involved in bioactivity, to the best of our knowledge, there is no research on the effects of Allium senescens on drug resistance in hepatocarcinoma. Ultra-high performance liquid chromatography was used to determine the concentration of several bioactive compounds in A. senescens extract; flow cytometry, reverse transcription–quantitative polymerase chain reaction, and siRNA-mediated knockdown to estimate the levels of different markers in HepG2 cells. The quantity of p-coumaric acid in the extract was 4.7291 ± 0.06 μg/mL, and the protein of relevant evolutionary and lymphoid interest (PRELI) in the resistant cells decreased 2.1 times in the presence of p-coumaric acid. The resistant cells strongly downregulated the efflux transporters (ABCB1, ABCC2, and ABCG2) when exposed to the extract or p-coumaric acid and when PRELI was knocked down, in contrast to the influx proteins (OCT-1). Additionally, the extract induced mitochondrial apoptosis and suppressed autophagy. Consequently, the extract and p-coumaric acid attenuated drug resistance of HepG2 cells through the downregulation of PRELI, a key protein associated with the modulation of drug transporter expression, the activation of autophagy, and mitochondrial apoptosis. Our results indicate that A. senescens extract is beneficial in protecting cancer cells against drug resistance and sustaining the efficacy of sorafenib against liver cancer.
Straight Gait Research of a Small Electric Hexapod Robot
Authors: Zhang Feng , Zhang Shidong , Wang Qian , Yang Yujie , Jin Bo
Published: April 2021

Gait is an important research content of hexapod robots. To better improve the motion performance of hexapod robots, many researchers have adopted some high-cost sensors or complex gait control algorithms. This paper studies the straight gait of a small electric hexapod robot with a low cost, which can be used in daily life. The strategy of “increasing duty factor” is put forward in the gait planning, which aims to reduce foot sliding and attitude fluctuation in robot motion. The straight gaits of the robot include tripod gait, quadrangular gait, and pentagonal gait, which can be described conveniently by discretization and a time sequence diagram. In order to facilitate the user to control the robot to achieve all kinds of motion, an online gait transformation algorithm based on the adjustment of foot positions is proposed. In addition, according to the feedback of the actual attitude information, a yaw angle correction algorithm based on kinematics analysis and PD controller is designed to reduce the motion error of the robot. The experiments show that the designed gait planning scheme and control algorithm are effective, and the robot can achieve the expected motion. The RMSE of the row, pitch, and yaw angle was reduced by 35%, 25%, and 12%, respectively, using the “increasing duty factor” strategy, and the yaw angle was limited in the range −3°~3° using the yaw angle correction algorithm. Finally, the comparison with related works and the limitations are discussed.
Selenium-Containing Polysaccharides—Structural Diversity, Biosynthesis, Chemical Modifications and Biological Activity
Authors: Górska Sandra , Maksymiuk Anna , Turło Jadwiga
Published: April 2021

Selenosugars are a group of sugar derivatives of great structural diversity (e.g., molar masses, selenium oxidation state, and selenium binding), obtained as a result of biosynthesis, chemical modification of natural compounds, or chemical synthesis. Seleno-monosaccharides and disaccharides are known to be non-toxic products of the natural metabolism of selenium compounds in mammals. In the case of the selenium-containing polysaccharides of natural origin, their formation is also postulated as a form of detoxification of excess selenium in microorganisms, mushroom, and plants. The valency of selenium in selenium-containing polysaccharides can be: 0 (encapsulated nano-selenium), IV (selenites of polysaccharides), or II (selenoglycosides or selenium built into the sugar ring to replace oxygen). The great interest in Se-polysaccharides results from the expected synergy between selenium and polysaccharides. Several plant- and mushroom-derived polysaccharides are potent macromolecules with antitumor, immunomodulatory, antioxidant, and other biological properties. Selenium, a trace element of fundamental importance to human health, has been shown to possess several analogous functions. The mechanism by which selenium exerts anticancer and immunomodulatory activity differs from that of polysaccharide fractions, but a similar pharmacological effect suggests a possible synergy of these two agents. Various functions of Se-polysaccharides have been explored, including antitumor, immune-enhancement, antioxidant, antidiabetic, anti-inflammatory, hepatoprotective, and neuroprotective activities. Due to being non-toxic or much less toxic than inorganic selenium compounds, Se-polysaccharides are potential dietary supplements that could be used, e.g., in chemoprevention.
Sustained Antibacterial Effect and Wear Behavior of Quaternary Ammonium Contact-Killing Dental Polymers after One-Year of Hydrolytic Degradation
Authors: Balhaddad Abdulrahman A. , Mokeem Lamia S. , Weir Michael D. , Xu Huakun , Melo Mary Anne S.
Published: April 2021

This study intended to investigate the long-term antibacterial effect, mechanical performance, and surface topography of new anticaries dental composites. While most artificial aging studies of dental resins lasted for 30–90 days, this study prolonged the water-aging to one year to be more clinically relevant. The base resin was loaded with dimethylaminohexadecyl methacrylate (DMAHDM) at 3 or 5 wt.% and nano-sized amorphous calcium phosphate (NACP) at 20 wt.%. Composites were subjected to one-year water storage and wear. Following water aging, samples were evaluated for flexural strength, elastic modulus, and microbiological assays. Biofilm plate counting method, metabolic assay, colorimetric quantification of lactic acid, and Baclight bacterial viability assay were measured after one year. Topography changes (ΔRa, ΔRq, ΔRv, ΔRt) were examined after wear and observed by scanning electron microscopy. Biofilm assays and topography changes data were analyzed via one-way ANOVA and Tukey’s tests. Mechanical properties and normalized data were verified using a t-test. The flexural strength values for the formulations that contained 5% DMAHDM-20% NACP, 3% DMAHDM, and 5% DMAHDM were reduced significantly (p < 0.05) in relation to the baseline but the values were still above the ISO standards. No significant differences were observed between the groups concerning the topography changes, except for the ΔRt, where there was a significant increase in the 5% DMAHDM-20% NACP group. All the groups demonstrated robust biofilm-inhibition, with slightly reduced antibacterial properties following water aging. The aged samples reduced the total microorganisms, total streptococci, and mutans streptococci by 1.5 to 3-log, compared to the experimental control. The new formulations containing DMAHDM and NACP were able to sustain the antibacterial performance after one-year of aging. Mechanical properties and surface topography were slightly affected over time.
Adversarial Optimization-Based Knowledge Transfer of Layer-Wise Dense Flow for Image Classification
Authors: Yeo Doyeob , Kim Min-Suk , Bae Ji-Hoon
Published: April 2021

A deep-learning technology for knowledge transfer is necessary to advance and optimize efficient knowledge distillation. Here, we aim to develop a new adversarial optimization-based knowledge transfer method involved with a layer-wise dense flow that is distilled from a pre-trained deep neural network (DNN). Knowledge distillation transferred to another target DNN based on adversarial loss functions has multiple flow-based knowledge items that are densely extracted by overlapping them from a pre-trained DNN to enhance the existing knowledge. We propose a semi-supervised learning-based knowledge transfer with multiple items of dense flow-based knowledge extracted from the pre-trained DNN. The proposed loss function would comprise a supervised cross-entropy loss for a typical classification, an adversarial training loss for the target DNN and discriminators, and Euclidean distance-based loss in terms of dense flow. For both pre-trained and target DNNs considered in this study, we adopt a residual network (ResNet) architecture. We propose methods of (1) the adversarial-based knowledge optimization, (2) the extended and flow-based knowledge transfer scheme, and (3) the combined layer-wise dense flow in an adversarial network. The results show that it provides higher accuracy performance in the improved target ResNet compared to the prior knowledge transfer methods.
Influence of Meteorological Conditions and Aerosol Properties on the COVID-19 Contamination of the Population in Coastal and Continental Areas in France: Study of Offshore and Onshore Winds
Authors: Piazzola Jacques , Bruch William , Desnues Christelle , Parent Philippe , Yohia Christophe , Canepa Elisa
Published: April 2021

Human behaviors probably represent the most important causes of the SARS-Cov-2 virus propagation. However, the role of virus transport by aerosols—and therefore the influence of atmospheric conditions (temperature, humidity, type and concentration of aerosols)—on the spread of the epidemic remains an open and still debated question. This work aims to study whether or not the meteorological conditions related to the different aerosol properties in continental and coastal urbanized areas might influence the atmospheric transport of the SARS-Cov-2 virus. Our analysis focuses on the lockdown period to reduce the differences in the social behavior and highlight those of the weather conditions. As an example, we investigated the contamination cases during March 2020 in two specific French areas located in both continental and coastal areas with regard to the meteorological conditions and the corresponding aerosol properties, the optical depth (AOD) and the Angstrom exponent provided by the AERONET network. The results show that the analysis of aerosol ground-based data can be of interest to assess a virus survey. We found that moderate to strong onshore winds occurring in coastal regions and inducing humid environment and large sea-spray production episodes coincides with smaller COVID-19 contamination rates. We assume that the coagulation of SARS-Cov-2 viral particles with hygroscopic salty sea-spray aerosols might tend to inhibit its viral infectivity via possible reaction with NaCl, especially in high relative humidity environments typical of maritime sites.
Proposal to Refine Solar Radiation of Typical Meteorological Year Database and Evaluation on the Influence of Air-Conditioning Load
Authors: Yuan Jihui , Emura Kazuo , Farnham Craig
Published: April 2021

The Typical meteorological year (TMY) database is often used to calculate air-conditioning loads, and it directly affects the building energy savings design. Among four kinds of TMY databases in China—including Chinese Typical Year Weather (CTYW), International Weather for Energy Calculations (IWEC), Solar Wind Energy Resource Assessment (SWERA) and Chinese Standard Weather Data (CSWD)—only CSWD is measures solar radiation, and it is most used in China. However, the solar radiation of CSWD is a measured daily value, and its hourly value is separated by models. It is found that the cloud ratio (diffuse solar radiation divided by global solar radiation) of CSWD is not realistic in months of May, June and July while compared to the other sets of TMY databases. In order to obtain a more accurate cloud ratio of CSWD for air-conditioning load calculation, this study aims to propose a method of refining the cloud ratio of CSWD in Shanghai, China, using observed solar radiation and the Perez model which is a separation model of high accuracy. In addition, the impact of cloud ratio on air-conditioning load has also been discussed in this paper. It is shown that the cloud ratio can yield a significant impact on the air conditioning load.
ERK1/2: An Integrator of Signals That Alters Cardiac Homeostasis and Growth
Authors: Gilbert Christopher J. , Longenecker Jacob Z. , Accornero Federica
Published: April 2021

Integration of cellular responses to extracellular cues is essential for cell survival and adaptation to stress. Extracellular signal-regulated kinase (ERK) 1 and 2 serve an evolutionarily conserved role for intracellular signal transduction that proved critical for cardiomyocyte homeostasis and cardiac stress responses. Considering the importance of ERK1/2 in the heart, understanding how these kinases operate in both normal and disease states is critical. Here, we review the complexity of upstream and downstream signals that govern ERK1/2-dependent regulation of cardiac structure and function. Particular emphasis is given to cardiomyocyte hypertrophy as an outcome of ERK1/2 activation regulation in the heart.
The Impact of COVID-19 on Depressive Symptoms through the Lens of Sexual Orientation
Authors: Duarte Mariana , Pereira Henrique
Published: April 2021

This research seeks to explore the impact of COVID-19 on depressive symptoms, analyzing discrepancies of sexual orientation in a Portuguese-speaking sample. 1590 individuals participated, of which 63% were women, and 88% self-identified as straight. Participants responded to the depression sub-scale of the Beck Symptoms Iventory-18, the fear of COVID-19 scale and the COVID-19 negative impact scale. Depressive symptoms observed were higher than expected, and several significant differences were obtained: women and self-identified bisexual participants had higher levels of depressive symptoms compared to male and straight and gay or lesbian participants. Depressive symptoms negatively correlated with age and positively correlated with COVID-19 aggravated responses, fear of COVID-19, and negative impact of COVID-19. Hierarchical linear regression analysis showed that age, gender and sexual orientation explained 6% of the variance of depressive symptoms, and when fear and the negative impact of COVID-19 was added, the model explained 23% of results. This study provides an important contribution to the understanding of factors arising from the pandemic that may have an impact on the mental health of sexual minorities.
Intersection of DNA Repair Pathways and the Immune Landscape Identifies PD-L2 as a Prognostic Marker in Epithelial Ovarian Cancer
Authors: Batman Samantha , Matsuo Koji , Mhawech-Fauceglia Paulette , Munro Elizabeth , Weisenberger Mercedes , Allen Allison , Joshi Sonali , Machida Hiroko , Matsuzaki Shinya , Bozanovic Tatjana , Pejovic Tanja
Published: April 2021

Background: Targeting DNA repair and immune checkpoint pathways has been the focus of multiple clinical trials. In this study, we explore the association between DNA repair proteins, immune response markers, and clinical outcome in women with EOC. Methods: Immunohistochemical analysis of TMA with 181 EOC samples was used to determine expression levels for DNA repair proteins (PARP, PTEN, p53, H2Ax, FANCD2, and ATM) and immune-markers (CD4, CD8, CD68, PD-L2, PD-L1, and FOXP3). Biomarker expression was correlated to clinical data. Prognostic discriminatory ability was assessed per the combination of biomarkers. Results: Tumor immunity biomarkers correlated with HRD biomarkers. High PD-L2 was significantly associated with high expression of CD8 (r = 0.18), CD68 (r = 0.17), and FOXp3 (r = 0.16) (all, p < 0.05). In a multivariate analysis, PD-L2 (hazard ratio (HR) 1.89), PARP (HR 1.75), and PTEN (HR 1.96) expressions were independently associated with decreased progression-free survival (PFS), whereas PD-L1 (HR 0.49) and CD4 (HR 0.67) were associated with improved PFS (all, p < 0.05). In 15 biomarker combinations, six combinations exhibited a discriminatory ability of >20% for the 4.5-year PFS rate, with four based on PD-L2 (PARP, PTEN, CD4, and PD-L1, 20.5–30.0%). Conclusions: Increased PD-L2 expression is a prognostic marker of decreased survival in EOC. Interaction between tumor DNA repair and microenvironment determines tumor progression and survival.
The Role of lncRNAs in the Pathobiology and Clinical Behavior of Multiple Myeloma
Authors: Carrasco-León Arantxa , Amundarain Ane , Gómez-Echarte Nahia , Prósper Felipe , Agirre Xabier
Published: April 2021

MM is a hematological neoplasm that is still considered an incurable disease. Besides established genetic alterations, recent studies have shown that MM pathogenesis is also characterized by epigenetic aberrations, such as the gain of de novo active chromatin marks in promoter and enhancer regions and extensive DNA hypomethylation of intergenic regions, highlighting the relevance of these non-coding genomic regions. A recent study described how long non-coding RNAs (lncRNAs) correspond to 82% of the MM transcriptome and an increasing number of studies have demonstrated the importance of deregulation of lncRNAs in MM. In this review we focus on the deregulated lncRNAs in MM, including their biological or functional mechanisms, their role as biomarkers to improve the prognosis and monitoring of MM patients, and their participation in drug resistance. Furthermore, we also discuss the evidence supporting the role of lncRNAs as therapeutic targets through different novel RNA-based strategies.
Inter-Reader Agreement of Diffusion-Weighted Magnetic Resonance Imaging for Breast Cancer Detection: A Multi-Reader Retrospective Study
Authors: Pesapane Filippo , Rotili Anna , Penco Silvia , Montesano Marta , Agazzi Giorgio Maria , Dominelli Valeria , Trentin Chiara , Pizzamiglio Maria , Cassano Enrico
Published: April 2021

Purpose: In order to evaluate the use of un-enhanced magnetic resonance imaging (MRI) for detecting breast cancer, we evaluated the accuracy and the agreement of diffusion-weighted imaging (DWI) through the inter-reader reproducibility between expert and non-expert readers. Material and Methods: Consecutive breast MRI performed in a single centre were retrospectively evaluated by four radiologists with different levels of experience. The per-breast standard of reference was the histological diagnosis from needle biopsy or surgical excision, or at least one-year negative follow-up on imaging. The agreement across readers (by inter-reader reproducibility) was examined for each breast examined using Cohen’s and Fleiss’ kappa (κ) statistics. The Wald test was used to test the difference in inter-reader agreement between expert and non-expert readers. Results: Of 1131 examinations, according to our inclusion and exclusion criteria, 382 women were included (49.5 ± 12 years old), 40 of them with unilateral mastectomy, totaling 724 breasts. Overall inter-reader reproducibility was substantial (κ = 0.74) for expert readers and poor (κ = 0.37) for non- expert readers. Pairwise agreement between expert readers and non-expert readers was moderate (κ = 0.60) and showed a statistically superior agreement of the expert readers over the non-expert readers (p = 0.003). Conclusions: DWI showed substantial inter-reader reproducibility among expert-level readers. Pairwise comparison showed superior agreement of the expert readers over the non-expert readers, with the expert readers having higher inter-reader reproducibility than the non-expert readers. These findings open new perspectives for prospective studies investigating the actual role of DWI as a stand-alone method for un-enhanced breast MRI.
Liquid Biopsy in Pancreatic Cancer: Are We Ready to Apply It in the Clinical Practice?
Authors: Heredia-Soto Victoria , Rodríguez-Salas Nuria , Feliu Jaime
Published: April 2021

Pancreatic ductal adenocarcinoma (PDAC) exhibits the poorest prognosis of all solid tumors, with a 5-year survival of less than 10%. To improve the prognosis, it is necessary to advance in the development of tools that help us in the early diagnosis, treatment selection, disease monitoring, evaluation of the response and prognosis. Liquid biopsy (LB), in its different modalities, represents a particularly interesting tool for these purposes, since it is a minimally invasive and risk-free procedure that can detect both the presence of genetic material from the tumor and circulating tumor cells (CTCs) in the blood and therefore distantly reflect the global status of the disease. In this work we review the current status of the main LB modalities (ctDNA, exosomes, CTCs and cfRNAs) for detecting and monitoring PDAC.
Down-Regulation of the Proteoglycan Decorin Fills in the Tumor-Promoting Phenotype of Ionizing Radiation-Induced Senescent Human Breast Stromal Fibroblasts
Authors: Mavrogonatou Eleni , Papadopoulou Adamantia , Fotopoulou Asimina , Tsimelis Stathis , Bassiony Heba , Yiacoumettis Andreas M. , Panagiotou Petros N. , Pratsinis Harris , Kletsas Dimitris
Published: April 2021

Down-regulation of the small leucine-rich proteoglycan decorin in the stroma is considered a poor prognostic factor for breast cancer progression. Ionizing radiation, an established treatment for breast cancer, provokes the premature senescence of the adjacent to the tumor stromal fibroblasts. Here, we showed that senescent human breast stromal fibroblasts are characterized by the down-regulation of decorin at the mRNA and protein level, as well as by its decreased deposition in the pericellular extracellular matrix in vitro. Senescence-associated decorin down-regulation is a long-lasting process rather than an immediate response to γ-irradiation. Growth factors were demonstrated to participate in an autocrine manner in decorin down-regulation, with bFGF and VEGF being the critical mediators of the phenomenon. Autophagy inhibition by chloroquine reduced decorin mRNA levels, while autophagy activation using the mTOR inhibitor rapamycin enhanced decorin transcription. Interestingly, the secretome from a series of both untreated and irradiated human breast cancer cell lines with different molecular profiles inhibited decorin expression in young and senescent stromal fibroblasts, which was annulled by SU5402, a bFGF and VEGF inhibitor. The novel phenotypic trait of senescent human breast stromal fibroblasts revealed here is added to their already described cancer-promoting role via the formation of a tumor-permissive environment.
Catalytic Combustion of Toluene over Highly Dispersed Cu-CeOx Derived from Cu-Ce-MOF by EDTA Grafting Method
Authors: Sun Wenjie , Huang Yijia , Li Xiaomin , Huang Zhen , Xu Hualong , Shen Wei
Published: April 2021

In this work, Cu-CeOx-MOF catalysts with well-dispersed Cu in different contents were synthesized via the ethylenediaminetetraacetic acid (EDTA) grafting method. EDTA was grafted in Ce-MOF-808 to anchor Cu and then the metal-organic frameworks (MOFs) were utilized as sacrificial template to form highly performed Cu-CeOx-MOF for toluene catalytic combustion. In this series of samples, Cu-CeOx-MOF-0.2 had a higher ratio of Oα/(Oα+Oβ), more oxygen vacancies and performed better low-temperature reducibility. Cu-CeOx-MOF-0.2 showed outstanding catalytic activity and stability. The T90 (temperature when toluene conversion achieved 90%) of Cu-CeOx-MOF-0.2 was 226 °C at 60,000 mL/(gcat∙h). In situ diffuse reflectance infrared transform spectroscopy (in situ DRIFTS) results revealed that the opening of aromatic ring and the deep oxidation of carboxylate were key steps for toluene catalytic combustion over Cu-CeOx-MOF-0.2.
Mechanism of Guaiacol Hydrodeoxygenation on Cu (111): Insights from Density Functional Theory Studies
Authors: Konadu Destiny , Kwawu Caroline Rosemyya , Tia Richard , Adei Evans , de Leeuw Nora Henriette
Published: April 2021

Understanding the mechanism of the catalytic upgrade of bio-oils via the process of hydrodeoxygenation (HDO) is desirable to produce targeted oxygen-deficient bio-fuels. We have used calculations based on the density functional theory to investigate the reaction mechanism of HDO of guaiacol over Cu (111) surface in the presence of H2, leading to the formation of catechol and anisole. Our analysis of the thermodynamics and kinetics involved in the reaction process shows that catechol is produced via direct demethylation, followed by dehydrogenation of –OH and re-hydrogenation of catecholate in a concerted fashion. The de-methylation step is found to be the rate-limiting step for catechol production with a barrier of 1.97 eV. Formation of anisole will also proceed via the direct dehydroxylation of guaiacol followed by hydrogenation. Here, the rate-limiting step is the dehydroxylation step with an energy barrier of 2.07 eV. Thermodynamically, catechol formation is favored while anisole formation is not favored due to the weaker interaction seen between anisole and the Cu (111) surface, where the binding energies of guaiacol, catechol, and anisole are -1.90 eV, −2.18 eV, and −0.72 eV, respectively. The stepwise barriers also show that the Cu (111) surface favors catechol formation over anisole as the rate-limiting barrier is higher for anisole production. For catechol, the overall reaction is downhill, implying that this reaction path is thermodynamically and kinetically preferred and that anisole, if formed, will more easily transform.
Proline Concentration and Its Metabolism Are Regulated in a Leaf Age Dependent Manner But Not by Abscisic Acid in Pea Plants Exposed to Cadmium Stress
Authors: Zdunek-Zastocka Edyta , Grabowska Agnieszka , Michniewska Beata , Orzechowski Sławomir
Published: April 2021

The accumulation of proline is one of the defense mechanisms of plants against the harmful effects of adverse environmental conditions; however, when pea plants were treated for 12 h with CdCl2, the proline concentration decreased in the youngest A (not expanded) and B1 (expanded) leaves, and did not change significantly in the B2 (mature, expanded) or C (the oldest) leaves. After 24 h of cadmium (Cd) stress, the proline concentration remained low in A and B1 leaves, while in B2 and C leaves, it increased, and after 48 h, an increase in the proline concentration in the leaves at each stage of development was observed. The role of proline in the different phases of plant response to the Cd treatment is discussed. Changes in proline accumulation corresponded closely with changes in the transcript levels of PsP5CS2, a gene encoding D1-pyrroline-5-carboxylate synthetase involved in proline synthesis, and PsPDH1, a gene encoding proline dehydrogenase engaged in proline degradation. CdCl2 application induced the expression of PsProT1 and PsProT2, genes encoding proline transporters, especially during the first 12 h of treatment in A and B1 leaves. When the time courses of abscisic acid (ABA) and proline accumulation were compared, it was concluded that an increase in the proline concentration in the leaves of Cd-treated pea plants was more related to a decrease in chlorophyll concentration (leaves B2 and C) and an increase in the malondialdehyde level (A and B1 leaves) than with an increase in ABA concentration alone. Exogenous application of ABA (0.5, 5, 50 µM) significantly increased the proline concentration in the A leaves of pea plants only, and was accompanied by an elevated and repressed expression of PsP5CS2 and PsPDH1 in these leaves, respectively. The presented results suggest that under Cd stress, the accumulation of proline in leaves of pea plants may take place independently of the ABA signaling.
Tailoring the Glass Composition to Increase the Thermal Stability without Impacting the Crystallization Behavior of Oxyfluorophosphate Glass
Authors: Ojha Nirajan , Dmitrieva Iuliia , Blanc Wilfried , Petit Laeticia
Published: April 2021

Even though the (75 NaPO3-25 CaF2) (in mol%) glass can be heat-treated into transparent glass-ceramic with Er3+ doped CaF2 crystals precipitating in the volume of the glass during heat-treatment, this glass was found to be a poor glass former, limiting its use as upconverter under 975 nm pumping. In this study, the impact of the glass composition on the thermal, optical and structural properties of the glass was investigated in order to understand how the glass composition can be tailored for the development of thermally stable upconverter glass-based material. The addition of MgO, Fe2O3 and Al2O3 in the NaPO3-CaF2 glass system increases the thermal stability of glass due to the depolymerization of the glass network. However, the changes in the glass composition also impacted on the nucleation and growth process. Indeed, CaF2 and other crystals were found in the newly developed glasses after heat-treatment leading to glass-ceramics with lower intensity of upconversion than the (75 NaPO3-25 CaF2) glass-ceramic used as a reference. Glasses were also prepared with different concentrations of Er2O3 and ErF3. These glasses were found to be promising as not only are they thermally stable, but they also exhibit green and red emission with high intensity under 975 nm pumping due to Er3+ clustering.
Comparative Effectiveness of Pediatric Integrative Medicine: A Pragmatic Cluster-Controlled Trial
Authors: Vohra Sunita , Punja Salima , Jou Hsing , Schlegelmilch Michael , Wilson Beverly , Spavor Maria , Grundy Paul , Mackie Andrew S. , Conway Jennifer , Hartfield Dawn
Published: April 2021

Symptoms of pain, nausea/vomiting, and anxiety (PNVA) are highly prevalent in pediatric inpatients. Poorly managed symptoms can lead to decreased compliance with care, and prolonged recovery times. Pharmacotherapy used to manage PNVA symptoms is of variable effectiveness and carries safety risks. Complementary therapies to manage these symptoms are gaining popularity due to their perceived benefits and low risk of harm. Pediatric integrative medicine (PIM) is the combination of complementary therapies with conventional medicine in pediatric populations. A two-arm, cluster-controlled, pragmatic clinical trial was carried out to compare the effectiveness of a PIM service in conjunction with usual care, versus usual care only to treat PNVA symptoms in hospitalized pediatric patients. The primary outcome was the improvement of PNVA symptom severity using a 10-point numerical rating scale. Participant enrollment occurred between January 2013 and January 2016. A total of 872 participants (usual care n = 497; PIM n = 375) were enrolled. The PIM therapies significantly reduced PNVA symptom severity (p < 0.001). This study found that a hospital-based PIM service is both safe and effective for alleviating PNVA symptoms. Future research should carry out this work in other pediatric inpatient divisions, and in other sites to determine the reproducibility of findings.
Complete Penetrance but Different Phenotypes in a Korean Family with Maternal Interstitial Duplication at 15q11.2-q13.1: A Case Report
Authors: Han Ji Yoon , Lee Hyun Joo , Lee Young-Mock , Park Joonhong
Published: April 2021

The 15q duplication syndrome (dup15q) is due to the presence of at least one additional derived copy of the Prader–Willi syndrome/Angelman syndrome (PWS/AS) critical region that is approximately 5 Mb long within chromosome 15q11.2-q13.1. This report describes distinct roles of the origin of interstitial (int) dup15q underlining the critical importance of maternally active imprinted genes in the contribution to complete penetrance but different phenotypes of neuropsychotic disorders such as schizophrenia (SCZ) and autism spectrum disorder (ASD) in a Korean family. The proband’s mother as a consultant visited our hospital for her offspring’s genetic counseling and segregation analysis. She had two daughters diagnosed as SCZ or ASD and one son diagnosed as ASD. To resolve the potential genetic cause of SCZ and ASD in the proband and her sibling, whole genomic screening of chromosomal rearrangements by array-comparative genomic hybridization (CGH) was performed using SurePrint G3 Human CGH + SNP Microarray 4 × 180 K. Results of the array-CGH analysis revealed an interstitial duplication at 15q11.2-q13.1 (duplication size of 5.4 Mb) in the mother and her three offspring with SCZ or ASD. Our case, together with previous findings of high occurrence of psychotic disorder, suggest that maternally expressed gene product in the critical region of PWS/AS might mediate the risk of neurodevelopmental disorder (ASD) as well as psychotic disorder (SCZ). Multiple cytogenetic and molecular methods are recommended for investigating children with 15q11.2-q13.1 duplication and neuropsychotic disorders.
Boredom in Adolescence: Validation of the Italian Version of the Multidimensional State Boredom Scale (MSBS) in Adolescents
Authors: Spoto Andrea , Iannattone Sara , Valentini Perla , Raffagnato Alessia , Miscioscia Marina , Gatta Michela
Published: April 2021

Boredom in adolescence is often underestimated, although it may be the sign of a profound unease or be associated with psychological disorders. Given the complexity of the construct of boredom and its increasing prevalence among adolescents in recent years, the present study aimed to validate the factorial structure of the Italian version of the Multidimensional State Boredom Scale (MSBS) in adolescents using a cross-validation approach. The study involved 272 students (33.8% males, 66.2% females) aged 14–19 (M = 15.9, SD = 1.38) living in northern and central Italy. In addition to the MSBS, the Symptoms Checklist 90-R (SCL 90-R) and the Children’s Depression Inventory (CDI) were administered. Exploratory and confirmatory factor analyses validated a 23-item structure of the MSBS, comprising five correlated factors. The tool showed a good internal consistency for these factors and a good convergent and factor validity. The MSBS consequently seems a valid and reliable method for assessing boredom in adolescence. The cut-off for the total score that could pinpoint cases posing a potential clinical risk was 88. A weak correlation was found between the total level of boredom and the daily Internet usage, while no relationship emerged between boredom and age, gender, and grades. Since excessive levels of boredom may conceal a general unease that could develop into structured psychological disorders, the value of the MSBS lies in enabling us to identify in advance adolescents at potential clinical risk.
Effectiveness and Compatibility of Nanoparticle Based Multifunctional Coatings on Natural and Man-Made Stones
Authors: Zuena Martina , Ruggiero Ludovica , Della Ventura Giancarlo , Bemporad Edoardo , Ricci Maria Antonietta , Sodo Armida
Published: April 2021

The interaction of microorganisms with stone materials leads to biodeterioration processes, which may cause aesthetic damages and the loss of durability and strength of the substrates. Innovative solutions against this process are represented by nanotechnologies. In our previous works, 2-mercaptobenzothiazole was successfully encapsulated within two silica-based nanodevices: nanocapsules and mesoporous nanoparticles. Such loaded nanodevices have been dispersed in TEOS based coatings, characterized as far as their chemical–physical properties and in vitro biocide efficacy. Here, we adopt a multi-technic approach, to assess the coatings efficacy and compatibility with four types of stones of cultural heritage interest, namely, mortar, brick, travertine, and Carrara marble. In particular, we determine the protective function of the coatings, based on water transport properties (reduction up to a factor 10 of the water absorption for brick and mortar, without significantly influencing water vapor transmission rate), morphology of the surface (absence of coating cracks and color changes), and TiO2 photocatalytic activity. Consequently, these coatings can be considered suitable for application on stone artifacts, without interfering with their artistic appearance.
Validating Anti-Infective Activity of Pleurotus Opuntiae via Standardization of Its Bioactive Mycoconstituents through Multimodal Biochemical Approach
Authors: Tiwari Pandey Aprajita , Pandey Ishan , Kanase Anurag , Verma Amita , Garcia-Canibano Beatriz , Dakua Sarada , Balakrishnan Shidin , Singh Mohan
Published: April 2021

Mushrooms produce a variety of bioactive compounds that are known to have anti-pathogenic properties with safer and effective therapeutic effects in human disease prognosis. The antibacterial activity of ethanol and methanol extracts of Pleurotus opuntiae were checked against pathogenic microorganisms viz. Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis NCIM 2300, Proteus vulgaris NCIM 5266, Serratia marcescens NCIM 2078, Shigella flexeneri NCIM 5265, Moraxella sp. NCIM 2795, Staphylococcus aureus ATCC 25923 by agar well diffusion method at different concentrations of the extracts. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts was determined by INT (Iodonitrotetrazolium chloride) colorimetric assay. Extracts were standardized by thin layer chromatography (TLC) in different solvent systems. The Retention factors (Rf) of different compounds were calculated by high performance TLC (HPTLC) fingerprinting at UV 254, 366, and 540 nm before and after derivatization. The ethanol and methanol extracts of P. opuntiae showed bactericidal activity against all the test pathogens at MIC values of 15.6 to 52.08 mg/mL and 20.81 to 52.08 mg/mL respectively. Whereas the MBC values for ethanol and methanol extract of P. opuntiae against all pathogens were recorded as 26.03 to 62.5 mg/mL and 125 mg/mL respectively. Preliminary mycochemical screening of both the extracts revealed high contents of bioactive compounds. Amongst all the solvent systems used in TLC, the best result was given by chloroform + hexane (8:2) which eluted out 5 different compounds (spots). HPTLC results revealed spots with different Rf values for all the 24 compounds present. Thus, it can be inferred from the present investigation that the mycoconstituents could be an alternative medication regimen and could play a role in new drug discoveries against different infections. Further, the antimicrobial components of these mushrooms can be transformed to bioengineered antimicrobial coatings for surfaces, drug and other hybrid systems for public health implications in combating persistent infections.
Stability, Elastic and Electronic Properties of Ta2N by First-Principles Calculations
Authors: Zhu Longpeng , Wang Jiong , Dong Chenchen , Du Yong , Shang Shun-Li , Liu Zi-Kui
Published: April 2021

Owing to exploring the influence of the N atoms ordering in Ta2N compounds on their properties, the stability, elastic, and electronic properties of Ta2N compounds (Ta2N-I: P3¯ml and Ta2N-II: P3¯1m) were investigated using first-principles calculations based on density functional theory. Ta2N-II is energetically favorable according to the enthalpy of formation. Elastic constants were employed to reveal the stronger resistance to deformation, but weaker anisotropy, in Ta2N-II. A ductile-brittle transition was found between Ta2N-I (ductile) and Ta2N-II (brittle). The partial density of states showed a stronger orbital hybridization of Ta-d and N-p in Ta2N-II, resulting in stronger covalent bonding. The charge density difference illustrated the interaction of the Ta-N bond and electron distribution of Ta2N.
UAVs Trajectory Optimization for Data Pick Up and Delivery with Time Window
Authors: Khoufi Ines , Laouiti Anis , Adjih Cedric , Hadded Mohamed
Published: April 2021

Unmanned Aerial Vehicles (UAVs), also known as drones, are a class of aircraft without the presence of pilots on board. UAVs have the ability to reduce the time and cost of deliveries and to respond to emergency situations. Currently, UAVs are extensively used for data delivery and/or collection to/from dangerous or inaccessible sites. However, trajectory planning is one of the major UAV issues that needs to be solved. To address this question, we focus in this paper on determining the optimized routes to be followed by the drones for data pickup and delivery with a time window with an intermittent connectivity network, while also having the possibility to recharge the drones’ batteries on the way to their destinations. To do so, we formulated the problem as a multi-objective optimization problem, and we showed how to use the Non-dominated Sorting Genetic Algorithm II (NSGA-II) to solve this problem. Several experiments were conducted to validate the proposed algorithm by considering different scenarios.
Few-Shot Learning with a Novel Voronoi Tessellation-Based Image Augmentation Method for Facial Palsy Detection
Authors: Abayomi-Alli Olusola Oluwakemi , Damaševičius Robertas , Maskeliūnas Rytis , Misra Sanjay
Published: April 2021

Face palsy has adverse effects on the appearance of a person and has negative social and functional consequences on the patient. Deep learning methods can improve face palsy detection rate, but their efficiency is limited by insufficient data, class imbalance, and high misclassification rate. To alleviate the lack of data and improve the performance of deep learning models for palsy face detection, data augmentation methods can be used. In this paper, we propose a novel Voronoi decomposition-based random region erasing (VDRRE) image augmentation method consisting of partitioning images into randomly defined Voronoi cells as an alternative to rectangular based random erasing method. The proposed method augments the image dataset with new images, which are used to train the deep neural network. We achieved an accuracy of 99.34% using two-shot learning with VDRRE augmentation on palsy faces from Youtube Face Palsy (YFP) dataset, while normal faces are taken from Caltech Face Database. Our model shows an improvement over state-of-the-art methods in the detection of facial palsy from a small dataset of face images.