bluuebunny/crossref_metadata_2025 huggingface dataset:
Pendidikan Mitigasi Bencana dan Kesiapsiagaan Anak dalam Menghadapi Gempa Bumi di SDN 09 Berok Nipah
Authors: Maharani Shania , Sari Rebbi Permata , Ikbal Revi Neini , Rahmi Hidayatul
Published: January 2025

Gempa bumi merupakan bencana alam yang sering kali menimbulkan korban jiwa, terutama pada anak-anak yang rentan akibat kurangnya pemahaman mengenai mitigasi bencana. SDN 09 Berok Nipah berada di garis tepi pantai Kota Padang yang memiliki 72 siswa yang menjadi kelompok rentan terhadap gempa. Tujuan penelitian untuk mengetahui pengaruh pendidikan mitigasi bencana terhadap kesiapsiagaan anak usia sekolah dalam  menghadapi gempa bumi di SDN 09 Berok Nipah. Penelitian menggunakan desain quasy eksperimen one grup pretest-postest. Populasi penelitian anak usia sekolah kelas II-V sebanyak 53 siswa. Proses penelitian dilakukan pada bulan Maret-Agustus 2024, teknik pengambilan sampel menggunakan random sampling sebanyak 34 responden. Pengumpulan data dilakukan menggunakan kuesioner pretest dan posttest setelah diberikan penyuluhan. Data dianalisis secara univariat dan bivariat menggunakan uji wilcoxon .Hasil penelitian rata-rata tingkat pengetahuan sebelum diberikan pendidikan mitigasi bencana 52,18, dan rata-rata pengetahuan sesudah diberikan pendidikan mitigasi bencana  91,06 selisih rata-rata pengatahuan 38,9. Ada pengaruh pendidikan mitigasi bencana gempa bumi terhadap kesiapsiagaan (p-0,000) pada anak usia sekolah di SDN 09 Berok Nipah tahun 2024. Kesimpulan : Diharapkan melalui kepala sekolah dan tenaga pengajar dapat memberikan informasi tentang mitigasi gempa dengan melaksanakan penyuluhan kesehatan dan simulasi bencana secara berkala setiap semester agar siswa mendapatkan pengetahuan yang baik.
Risiko Obesitas dengan Penyakit Stroke di Asia: Studi Meta-Analisis
Authors: Hasnah Fadhilatul
Published: January 2025

Penelitian ini menggunakan metode systematic review dan meta-analisis untuk melihat risiko obesitas dengan penyakit stroke di Asia. Analisis lebih lanjut berdasarkan subtipe stroke juga dilakukan. Pencarian literatur dilakukan pada database PubMed, EBSCO dan Google Scholar. Tes Q dilakukan untuk menentukan heterogenitas studi yang dimasukkan. Funnel Plot, uji Regresi Egger dan metode Trim and Fill digunakan untuk mengidentifikasi bias publikasi dan dengan transformasi model antara Fixed Effect Model dan Random Effect Model untuk analisis sensitivitas. Hasil analisis data yang ditampilkan pada forest plot, menunjukkan bahwa adanya hubungan obesitas dengan kejadian stroke dengan nilai p= 0.002 dan nilai pooled odds ratio sebesar 1,68 (95% CI 1,21-2,33), sehingga dapat disimpulkan bahwa orang yang didiagnosa obesitas berisiko 1,68 kali mengalami kejadian stroke dibandingkan orang yang tidak obesitas. Pada tes bias publikasi menggunakan Egger’s regression test, diperoleh nilai intercept tidak sama dengan nol yaitu 3,88384. Berdasarkan hasil Egger’s test, juga mengindikasikan adanya pengaruh bias publikasi pada hubungan obesitas dengan penyakit stroke.
Evaluasi Efektivitas Program Posyandu Lanjut Usia (Lansia) di Kelurahan Sumpang Binangae
Authors: Muhajirin Musdalifah , Majid Makhrajani , Umar Fitriani , Haniarti Haniarti
Published: January 2025

Posyandu lansia adalah pusat pelayanan kesehatan yang ditujukan bagi lansia, namun pemanfaatannya di Kelurahan Sumpang Binangae masih rendah. Tujuan penelitian ini untuk mengevaluasi pelaksanaan dan pemanfaatan posyandu lansia di Kelurahan Sumpang Binangae Kabupaten Barru, Jenis penelitian adalah kualitatif dengan pendekatan survei. Penelitian dilakukan pada bulan Februari-Maret 2023. Informan dalam penelitian ini sebanyak 18 orang terdiri dari informan kunci yaitu Kepala Puskesmas 1 orang, Petugas Kesehatan lansia 1 orang dan kader posyandu 4 orang terdiri dari kader Manunggal, Maju, Bahagia dan Jaya. Informan biasa yaitu lansia sebanyak 12 orang. Pemilihan informan dengan metode snowball sampling. Hasil penelitian menunjukkan bahwa pelaksanaan posyandu lansia di Kelurahan Sumpang Binangae Kabupaten Barru dilakukan secara efektif dengan penentuan jadwal pelaksanaan posyandu yang rutin serta pelayanan kesehatan secara maksimal. Kegiatan dilakukan mulai dari pendaftaran, pemeriksaan serta pengobatan dan pencegahan pada Lansia baik di posyandu maupun melalui program kunjungan rumah lansia. Pemanfaatan posyandu lansia dikategorikan rendah disebapkan oleh beberapa faktor penghambat diantaranya yaitu dukungan keluarga yang kurang dan pengetahuan lansia yang masih kurang dilihat dari kurang efektifnya mereka melakukan kunjungan dan melakukan pemeriksaan ke posyandu lansia. Disarankan kepada pengelola Posyandu lansia agar memberikan sosialisasi dengan pendekatan kekeluargaan sehingga lansia dan keluarganya dapat mendukung seluruh program kegiatan di posyandu
Aspek Sosial Budaya Terhadap Pemanfaatan Posyandu Lansia di Wilayah Kerja Puskesmas Bilokka
Authors: Suwe Andi Tenri , Usman Usman , Majid Makhrajani , Umar Fitriani
Published: January 2025

Aspek sosial budaya dari pengetahuan, kebiasaan dan kepercayaan Lansia pada umumnya mempengaruhi minat dan keinginanan untuk memanfaatkan Posyandu Lansia. faktor utama menurunnya tingkat pemanfaatan posyandu Lansia di Puskesmas Bilokka Kec. Pancalautang Kab. Sidrap yang menjadi alasan penelitian ini. Tujuan penelitian untuk mendeskripsikan aspek sosial budaya dilihat dari pengetahuan, kepercayaan dan kebiasaan lansia dalam memanfaatkan posyandu lansia. Jenis penelitian ini kualitatif yang dilaksanakan pada bulan Maret-April 2023. Informan dalam penelitian ini Kepala Puskesmas sebagai informan kunci, dan informan biasa yaitu petugas kesehatan 1 orang, Kader 4 orang dan Lansia 8 orang serta tokoh masyarakat 2 orang. Pemilihan informan dengan metode snowball sampling. Hasil penelitian ini menunjukkan pengetahuan lansia tergolong rendah dengan tingkat kesadaran pemanfaatan posyandu Lansia yang minim. Aspek sosial budaya dilihat dari kepercayaan lansia dalam memanfaatkan posyandu yaitu kepercayaan Lansia masih kuat pada keyakinan bahwa pemanfaatan posyandu Lansia hanya dilakukan ketika Lansia memiliki gangguan kesehatan berat. Aspek sosial budaya menunjukkan bahwa kebiasaan berdasarkan pengalaman pengobatan yang dilakukan oleh Lansia lebih dipilih dari pada pemeriksaan melalui posyandu Lansia sehingga minat mereka berkurang dan kurangnya dukungan keluarga dalam menanamkan kebiasaan datang ke posyandu lansia. disaran untuk tetap melakukan pendampingan dan pemeriksaan kepada lansia yang datang ke Posyandu.
Exploring the role of locally-sourced ingredients in the rural hospitality system of Hungary
Authors: Csapody Bence
Published: January 2025

The aim of this research is to examine the role of local ingredients in the Hungarian rural hospitality system and to analyse the benefits and drawbacks of their utilization in restaurants. Previous literature lacks comprehensive studies investigating the use of local products in restaurants and the collaboration between producers and restaurants, particularly those employing qualitative methods involving both parties. To address this research gap, sixteen interviews were conducted with restaurant representatives and local producers. The findings of this research contribute to understanding the structural barriers hindering the effective integration of local products in restaurant operations. The study reveals that the dynamic nature of restaurant offerings relying on local ingredients, poses challenges for chefs, requiring constant innovation. Additionally, factors such as reliability, transparency, and hyper-local procurement strategies play crucial roles in the adoption of local products. However, logistics and communication emerge as significant shortcomings in restaurant-producer relations, and addressing these issues could improve efficiency, financial outcomes, and product quality. Furthermore, the study provides practical recommendations for enhancing the efficiency of short value chains and fostering mutual understanding between producers and restaurants.
UNVEILING ASTROTOURISTS’ PROFILES. ACTIVITIES, MOTIVATIONS, ECONOMIC AND SOCIAL BENEFITS AND EXPERIENCES
Authors: Solano-Sánchez Miguel Ángel , Aja-Valle Jaime , Arteaga-Sánchez Rocío , López-Guzmán Tomás
Published: January 2025

This research aims to determine the variations that occur in the socio-demographic profile (gender, age, educational level and income) of the visitor interested in astrotourism based on some questions about the activities they carry out related to astronomy, motivations, economic and social benefits and the assessment of experiences about astrotourism through data gathered by a questionnaire. An artificial neural network is developed to generate potential “identikits” or estimated profiles of astrotourists based on the replies incorporated into the model and easily customisable by the researcher, this being especially useful in the development of products and services adapted to astrotourism.
The Analysis of Competitiveness and Export Performance of Indonesia's Frozen Shrimp Commodity (HS 030617) to Sweden
Authors: Manalu Doni Sahat Tua , Wijaya Allieccia Tessalonika , Aditia Derry
Published: January 2025

Udang beku Indonesia, khususnya di pasar Eropa termasuk Swedia, merupakan salah satu komoditas ekspor perikanan unggulan karena memiliki umur simpan yang panjang dan aman dalam transportasi. Meskipun memiliki keunggulan tersebut, kinerja ekspor udang beku Indonesia baru-baru ini menunjukkan penurunan yang memprihatinkan, mencerminkan perlunya penyesuaian strategi. Penelitian ini mengatasi tantangan ini dengan menganalisis daya saing udang beku Indonesia di pasar Uni Eropa serta mengidentifikasi area peningkatan guna menghadapi persaingan global yang semakin ketat. Menggunakan data ekspor dari tahun 2018 hingga 2022, penelitian ini menerapkan metode Revealed Comparative Advantage (RCA) untuk mengevaluasi posisi kompetitif Indonesia di pasar Swedia. Hasil penelitian menunjukkan bahwa meskipun udang beku Indonesia tetap kompetitif, komoditas ini menghadapi persaingan ketat dari negara seperti Ekuador dan India. Oleh karena itu, studi ini merekomendasikan penguatan daya saing melalui diversifikasi produk, inovasi pengolahan, dan peningkatan promosi untuk menjaga stabilitas dan pertumbuhan ekspor udang beku Indonesia di Uni Eropa.
Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains
Authors: Sánchez-Quintero Ángela , Parsy Aurélien , Adrien Amandine , Spitzer Lea , Jiménez-Lamana Javier , Fernandes Susana C. M. , Beigbeder Jean-Baptiste
Published: January 2025

This study evaluated the growth performance of Tetradesmus obliquus and Chlorella vulgaris microalgae cultivated in diluted liquid digestate supplemented with CO2, comparing their efficiency to that of a conventional synthetic media. The presence of an initial concentration of ammonium of 125 mg N-NH4+.L-1 combined with the continuous injection of 1% v/v CO2 enhanced the optimal growth responses and bioremediation potential for both strains in 200-mL cultures. In 6-L flat panel reactors, T. obliquus exhibited superior biomass production, achieving a final biomass concentration of 1.29 ± 0.06 g.L-1, while C. vulgaris reached only 0.36 ± 0.02 g.L-1. Both strains effectively contributed to the bioremediation of the digestate-based culture media, with up to 100% of N-NH4+, 50% of COD, and 55% of P-PO43- removals. The high nitrogen levels in the digestate-based medium significantly increased protein content, with 46.21% ± 3.98% dry weight (DW) for T. obliquus and 44.17% ± 2.24% DW for C. vulgaris as compared to the microalgae cultivated in commercial media. Additionally, the metal content of the microalgal biomass was analyzed to assess its potential use as biostimulants in compliance with European regulations. While chromium concentrations slightly exceeded regulatory thresholds in both strains, the levels of other metals remained within permissible limits.
Two previously undescribed triterpenoid saponins from the roots and rhizomes of Caulophyllum robustum Maxim
Authors: Zhang Cong-Yu , Wang Yu-Mei , Qiu Peng-Cheng , Feng Jia-Yu , Wang Bing-Wen , Cao Yu , Wei Yi-Sha , Zhou Yi-Tong , Tang Hai-Feng , Lu Yun-Yang , Zhang Qian
Published: January 2025

Since ancient times, plants have provided humans with important bioactive compounds for the treatment of various diseases. Nine compounds were isolated from the roots and rhizomes of Caulophyllum robustum (a plant in the family Panaxaceae), including two new saponins C. Spanion A and C. Spanion B (1-2) and seven known saponins (3-9). The cytotoxicity of these compounds on human cancer cell lines was analyzed using MTT method. Compounds 6 and 9 exhibit cytotoxicity towards these three types of human cancer cells (<10 μM). By utilizing the SEA platform for target prediction, a common tumor related target CD81 was identified. The molecular docking of saponins 1, 2, 6, and 9 with CD81 protein showed strong binding affinities ranging from -4.5 to -7.1 kcal/mol. Research has shown that these compounds can become potential anti-tumor drugs. Further research is still recommended to understand its exact molecular mechanism and toxicological effects.
The interplay between sex, lifestyle factors and built environment on 20-year cardiovascular disease incidence; the ATTICA study (2002–2022)
Authors: Sigala Evangelia G. , Chrysohoou Christina , Barkas Fotios , Liberopoulos Evangelos , Sfikakis Petros P. , Faka Antigoni , Tsioufis Costas , Pitsavos Christos , Panagiotakos Demosthenes
Published: January 2025

Background and aim

This study aims to investigate the role of the built environment in terms of urban-rural disparities in cardiovascular disease (CVD) epidemiology, focusing on middle- and long-term CVD risk assessment. Moreover, this study seeks to explore sex-specific differences in urban and rural settings.

Methods

The ATTICA Study is a prospective study conducted from 2002 onwards. At baseline, a random sample of 3,042 CVD-free adults (49.8% men) were randomly drawn from the population of the Attica region, in Greece, with 78% dwelling in urban and 22% in rural municipalities. Follow-up examinations were performed in 2006, 2012, and 2022. Of the total participants, 1,988 had complete data for CVD assessment in the 20-year follow-up.

Results

The 10-year and 20-year CVD incidence was 11.8%, 28.0% in rural municipalities and 16.8%, 38.7% in urban municipalities, respectively (ps < 0.05). Unadjusted data analyses revealed significant differences in clinical, laboratory, and lifestyle-related CVD risk factors between urban and rural residents (ps < 0.05). Additionally, sex-based discrepancies in clinical, anthropometric, circulating, and lifestyle risk factors were observed in stratified analyses of urban and rural settings. Multivariate analyses, including generalized structural equation modeling (GSEM), revealed that the impact of the urban built environment on the long-term (20-year) CVD risk is mediated by lifestyle-related risk factors.

Conclusion

Urban inhabitants exhibit a higher long-term CVD incidence compared to their rural counterparts, which was partially explained by their lifestyle behaviors. Targeted strategic city planning efforts promoting healthier lifestyle-related behaviors at the micro-environment level could potentially mitigate built-environment impacts on CVD health.
Exposure to specific polyfluoroalkyl chemicals is associated with cardiovascular disease in US adults: a population-based study
Authors: Xiao Wenwen , Jian Guojin , Ma Fei , Li Hong , Yang Xiaohong , Zhang Hengyang , Cao Yongping
Published: January 2025

Background

Polyfluoroalkyl chemicals (PFCs) present potential health risks due to their persistence and bioaccumulation. However, there is currently insufficient evidence regarding their impact on cardiovascular disease (CVD). Consequently, it is imperative to investigate the correlation between PFCs and CVD.

Methods

The data was collected from National Health and Nutrition Examination Survey in 2005–2012. Logistic regression models were employed to assess the association between single PFC and CVD. Generalized additive model (GAM) was used for evaluating nonlinear relationships. Subgroup analyses were conducted to explore interaction effects. Bayesian kernel machine regression (BKMR) and weighted quantile sum (WQS) models were used to evaluate the joint effect of PFC exposures on CVD.

Results

In logistic regression, PFDE, MPAH, and PFUA were positively associated with CVD. In the GAM, there was a significant nonlinear relationship between MPAH and CVD. Subgroup analysis revealed the interaction of gender and race in the effects of PFCs and CVD. PFUA was positively correlated with CVD in males but show no significant difference in females. PFDE was positively associated with CVD among non-Hispanic white individual. The results of BKMR indicated that the impact of mixed PFCs on CVD increased initially and then weakened, showing an overall positive trend. The results of WQS suggested that PFDO contributed most to the effect.

Conclusion

Our study showed that serum PFDE, MPAH, and PFUA levels were positively correlated with CVD. PUFA was found to interact with gender and race in relation to CVD. A general positive correlation exists between mixed exposure to PFCs and CVD, with PFDO being the most contributory PFC. Our study provided important evidence for probing the impact of PFCs on CVD and laid a foundation for further mechanism research.
Rice stable carbon and nitrogen isotope values as potential indicators of watering and manuring regimes
Authors: Wu Rubi , Belfield Eric J. , Dong Yu , Feng Shangzong , Fu Xiangdong , Harberd Nicholas P. , Yang Fan , Charles Michael , Bogaard Amy
Published: January 2025

Rice (Oryza sativa L.) is an important cereal crop across East, South and Southeast Asia, from prehistory to today, and grows in a range of ecological conditions, from rainfed upland to deep water. Previous research on early rice in the Lower Yangtze River basin (LYRB) suggested shifts back and forth over time between wet and dry field conditions, and some application of animal dung and/or human feces as fertilizer. In this study, stable carbon and nitrogen isotope analysis was conducted on grains from rice of heritage landraces and modern varieties grown in a glasshouse pot experiment with three watering levels and two manuring levels, and from recent rice fields in China and South Korea including paddy and dry conditions, and with variable manuring histories. The pot experiment results indicate that there is a negative correlation between the δ13C values and watering levels in one heritage accession and three modern accessions; in the remaining two heritage accessions and one modern accession, a similar trend in δ13C values is observed between watering levels. The rice field results show that, similarly, the δ13C values of rice from paddy fields were significantly lower than those from dry fields. The results from the pot experiment also indicate that both watering and manuring tend to increase the δ15N values of rice grains, while the field samples show that intensive manuring in initial growing seasons does not have a consistent effect on rice δ15N values. Overall, stable carbon and nitrogen isotope values of rice grains appear to be useful indicators of water status, with potential in combination to disentangle watering and manuring practices.
Comparative study on blasting simulation vibration reduction and field vibration reduction experiment of adjacent oil pipeline
Authors: Chen Yi , Zhang Ruichong , Liang Lujia , Wang Yi , Chen Jiaozhong , Xie Chengyu
Published: January 2025

Introduction

Yongxing Tunnel No.1’s complex geology near a buried oil pipeline on the Guizhou-Nanning high-speed route poses blasting risks. Prioritizing pipeline safety, efforts focus on minimizing vibration impacts.

Methods

Research uses numerical simulations and field tests to analyze three delay times and three spacing charge materials for vibration reduction.

Results

Optimal delay is 30 ms, yielding a 0.52 cm/s vibration velocity, 20%-29% lower than 28 ms and 32 ms. Soil is the most effective spacer, achieving 0.46 cm/s, 30.30%-22.03% lower than water and air. Field tests align with simulations.

Discussion

Findings provide a reference for optimal vibration reduction and safe construction under similar conditions.
Democracy is at risk: beliefs of Chilean teachers about the transmission of hate speech in teacher education
Authors: Marolla-Gajardo Jesús
Published: January 2025

Conversations about hate speech are a complex issue. It is not a new problem; on the contrary, society has been confronted with hate speech against specific communities at different moments. The present study aims to investigate the beliefs of Chilean teachers working in teacher education and their relationship with hate speech that may have occurred in their practice. The methodology was quantitative. The participants were 200 teachers. The data collection instrument was a survey to determine teachers’ beliefs. The results showed that teachers expressed concern about the problem and stated that action must be taken to combat hate speech. At the same time, they argued that their colleagues perpetuate and reproduce hate speech in their practice, which is also a complex situation that needs to be addressed. Finally, there is also a controversy about the limits of freedom of expression.
Efficacy of cartilage-targeted IGF-1 in a mouse model of growth hormone insensitivity
Authors: Tailor Krishma , van Ree Janine , Stowe Timothy , Ventura Brit , Sisk Connor , Courtis Joanna , Camp Anna , Elzamzami Fatima , van Deursen Jan , O’Brien Robert , Baron Jeffrey , Lui Julian C.
Published: January 2025

Recombinant human IGF-1 is used to treat severe primary IGF-1 deficiency, but this treatment requires twice-daily injection, often does not fully correct the growth deficit, and has important off-target effects. We therefore sought to target IGF-1 to growth plate cartilage by generating fusion proteins combining IGF-1 with single-chain human antibody fragments that target matrilin-3, a cartilage matrix protein. We previously showed that this cartilage-targeting IGF-1 fusion protein (CV1574-1) promoted growth plate function in a GH-deficient (lit) mouse model. Here, we studied CV1574-1 in a second mouse model, C57BL/6 wild-type mice treated with pegvisomant to induce GH resistance. In this model, once-daily injections of CV1574-1 for 5 days partially restored the pegvisomant-induced decrease in growth plate height without increasing kidney cell proliferation. Furthermore, we found that subcutaneous CV1574-1 showed significantly reduced hypoglycemic effect compared to injection of IGF-1 itself. Lastly, to gain mechanistic insights into the role of matrilin-3 targeting, we assessed the ability of CV1574-1 to activate AKT signaling in vitro and found that CV1574-1 caused a prolonged increase in AKT signaling compared to IGF-1 and that this effect was dependent on matrilin-3. Taken together, our findings provide further evidence that cartilage-targeted therapy could provide new pharmacological approaches for the treatment of childhood growth disorders, such as GH-insensitivity syndrome.
Impact of the fishing ban on fish diversity and population structure in the middle reaches of the Yangtze River, China
Authors: Du Juan , Tian Huiwu , Xiang Zhiyuan , Zhao Kangshun , Yu Lixiong , Duan Xinbin , Chen Daqing , Xu Jun , Liu Mingdian
Published: January 2025

The Yangtze River has experienced severe ecological degradation due to intensive human activities, including dam construction, land reclamation, and overfishing. These disturbances have disrupted the natural habitats of the Yangtze River, leading to a sharp decline in fish biodiversity and fishery resources. To address this ecological crisis, the Chinese government implemented a 10-year fishing ban in January 2021 to mitigate pressures on fish populations, restore aquatic habitats, and promote biodiversity recovery. The middle reaches of the Yangtze River are characterized by diverse fish species and a critical habitat for aquatic life, this study seeks to assess the effects of the fishing ban on fish diversity, body structure, population and community dynamics in this region. Fish monitoring data collected from 2017–2019 (pre-ban) and 2021–2023 (post-ban) were analyzed to evaluate changes in fish body size, species diversity, and community structure. The analysis results using the PSD method indicate that fish body size has increased following the fishing ban, suggesting the improvement of population structures, and a change in the complexity of food web structure. Species diversity indices showed partial recovery, but the recovery was uneven across different sampling sites. While fish populations showed signs of improvement, particularly in terms of body size and community stability, species diversity remained at relatively low levels in some areas, indicating that full recovery in biodiversity and resource levels may require extended conservation efforts. These findings suggest that while the fishing ban has had a positive initial impact on fish populations and ecological conditions, continued and long-term conservation measures are essential for fully recovering the river’s biodiversity and restoring its fishery resources. The study also highlights the importance of monitoring fish species diversity, body structure, and community dynamics as part of ongoing efforts to evaluate the effectiveness of the fishing ban and refine resource management strategies for the middle reaches of the Yangtze River.
Preparation of phosphogypsum ecological concrete and study on its phytogenic properties
Authors: Liu Rongxin , Liu Chunduo , Du Jiang , Wang Cheng , Yuan Yonghui , Zhang Xin
Published: January 2025

In this study, vegetative eco-concrete was prepared based on electrolytic manganese slag/phosphogypsum composite cementitious material as binder and clayey ceramic grains as aggregate. Based on the conditions of porosity and water-cement ratio, the optimal proportion of phosphogypsum-based eco-concrete was investigated, and concrete specimens with good performance were prepared (14 days compressive strength: 3.49 MPa, permeability coefficient: 1.37, total porosity: 24.5%, Improved compressive strength by 15% and water retention by 20%). The nutrient matrix of vegetative eco-concrete with different phosphogypsum/electrolytic manganese slag ratios was designed and modified, and the vegetative performance of the eco-concrete was investigated using four-season grass, ryegrass, clippings and clover as the grass species. The results showed that the eco-concrete based on phosphogypsum as raw material was rich in nutrients such as nitrogen, phosphorus and potassium, which could meet the requirements of plant growth, Supporting plant growth with a 30% increase in root length and 25% improvement in biomass compared to control concrete. The addition of improvers had a good passivation effect on heavy metals such as As, Cu, Cr, Zn, Sb and Pb in the phytogenic substrate. The adaptability of different grass species to the planting substrate was Four Seasons > ryegrass > shepherd’s purse > clover, alfalfa, dogbane; the application of electrolytic manganese slag substrate had the best performance of planting, and the planting substrate with the application of improver inhibited the growth of plants. The study addresses the challenge of using phosphogypsum as a binder in concrete, which has traditionally faced issues with strength and stability. By optimizing the mix ratio and curing process, we were able to achieve a concrete material that not only performs well mechanically but also supports plant growth.
Variability of PRDM9 in buffaloes
Authors: Santana Luca Godoi Rocha , Alves Jackeline Santos , Feitosa Fabieli Loise Braga , Rocha Victoria Camilla Parente , Tonhati Humberto , Costa Raphael Bermal , de Camargo Gregório Miguel Ferreira
Published: January 2025

The buffalo population raised in Brazil tend to show loss of genetic variability over generations, with significant estimates of inbreeding depression. Besides mating genetically distant individuals, other tools can be used to maintain/increase the genetic variability of the population, such as the use of PRDM9 genotypes. The PRDM9 gene promotes the creation of crossing-over points across the genome, with each allele promoting the creation of a different hotspot. Thus, increasing the frequency of less frequent alleles in the population, allows the emergence of new haplotypes and increases genetic variability. So, this study aimed to characterize the alleles of the PRDM9 gene circulating in the Murrah, Jaffarabadi, and Mediterranean breeds and verify their potential impact on genetic diversity management within the populations. The three alleles (B, C and D) were found in the three breeds at different frequencies, as well as the genotypic frequencies. The mating of different homozygous genotypes and genotypes carrying less frequent alleles may increase recombination rates and population variability. Four described variants and one new variant for allele D were found by sequencing. It was verified that it is possible to mate sires and dams with different PRDM9 genotypes in order to try to increase genetic variability in buffalo populations, improving the matings choices in buffalo breeding, helping to maintain production levels.
Streptococcus intermedius promotes synchronous multiple primary lung cancer progression through apoptosis regulation
Authors: Deng Yi , Dong Zhi Xiang , Yang Gao Hua , Krimsky William S. , Tai Yong Hang , Peng Hao , Huang Gui Ting , Xu Jia Xin , Sarkar Saiyad A. , Peng Jun , Qian Kai
Published: January 2025

Background

Dysbiosis of the lung microbiome can contribute to the initiation and progression of lung cancer. Synchronous multiple primary lung cancer (sMPLC) is an increasingly recognized subtype of lung cancer characterized by high morbidity, difficulties in early detection, poor prognosis, and substantial clinical challenges. However, the relationship between sMPLC pathogenesis and changes in the lung microbiome remains unclear.

Methods

In this study, 16S rRNA sequencing was performed on clinical samples to analyze lung microbiome composition. Real-time quantitative PCR (qPCR) was used to quantify bacterial abundance in lung tissues. In addition, flow cytometry was conducted to evaluate cell cycle progression and apoptosis in lung tumor cells.

Results

Clinical cohort studies demonstrated that sMPLC occurrence is associated with disturbances in the lung microbiome. Notably, Streptococcus intermedius was enriched in the lungs of sMPLC patients compared with non-tumor controls and accumulated preferentially in tumor tissues. S. intermedius shortened the cell cycle and inhibited apoptosis in lung cancer cells. Analyses of oral and gut microbiomes in different patient cohorts revealed a strong correlation between oral microbiome imbalances and lung microbiome composition in sMPLC patients.

Conclusions

These findings characterize the lung microbiota in sMPLC and identify S. intermedius as a potentially influential bacterial strain. This study provides significant new insights into the diagnosis and treatment of sMPLC.
Extracellular matrix shapes cancer stem cell behavior in breast cancer: a mini review
Authors: Li Lei , Tang Yidan , Qiu Ling , Li Zhengrui , Wang Ruo
Published: January 2025

Today, cancer has become one of the leading global tragedies. It occurs when a small number of cells in the body mutate, causing some of them to evade the body’s immune system and proliferate uncontrollably. Even more irritating is the fact that patients with cancers frequently relapse after conventional chemotherapy and radiotherapy, leading to additional suffering. Scientists thereby presume that cancer stem cells (CSCs) are the underlying cause of metastasis and recurrence. In recent years, it was shown that not only can chemotherapy and radiotherapy underperform in the treatment of breast cancer, but they can also increase the number of breast cancer stem cells (BCSCs) that transform regular breast cancer cells into their own population. Such data somewhat support the aforementioned hypothesis. Meanwhile, our understanding of the extracellular matrix (ECM) has changed considerably over the last decade. A lot of studies have bit by bit complemented human knowledge regarding how the ECM greatly shapes the behaviors of BCSCs. In this review, we highlighted the influence on BCSCs exerted by different critical components and biochemical properties of ECM.
Synergistic defecation effects of Bifidobacterium animalis subsp. lactis BL-99 and fructooligosaccharide by modulating gut microbiota
Authors: Zhang Qi , Zhao Wen , Luo Jie , Shi Shaoqi , Niu Xiaokang , He Jian , Wang Yicheng , Zeng Zhaozhong , Jiang Qiuyue , Fang Bing , Chen Juan , Li Yixuan , Wang Fuqing , He Jingjing , Guo Jie , Zhang Ming , Zhang Liwei , Ge Shaoyang , Hung Wei-Lian , Wang Ran
Published: January 2025

Introduction

Synbiotics have revealed the possibility of improving constipation through gut microbiota. The synergistic efficacy of Bifidobacterium animalis subsp. lactis BL-99 (BL-99) and fructooligosaccharide (FOS) on constipation have not been investigated.

Methods

Loperamide-induced constipated mice model was established to explore the effect of BL-99, FOS, and BL-99+FOS on changes of defecation-related parameters, gut microbiota and metabolites.

Results and discussion

The results showed that BL-99, FOS, and BL-99+FOS each alleviated constipation, with the synbiotic showing significant efficacy in the first black stool defecation time, fecal number, fecal weight, and the gastrointestinal transit rate (P < 0.05). Additionally, significant increased in serum 5-HT and IL-10 were observed in the BL-99+FOS group, alongside an increased relative abundance of Lachnospiraceae_NK4A136_group, Blautia, and Clostridium sensu stricto 1, while significantly reducing the relative abundance of Alistipes and Bacteroides. These changes facilitated alterations in short-chain fatty acids (SCFAs) metabolism, and were closely associated with the expression of genes related to the 5-HT pathway and the modulation of serum inflammatory factors. This study provides a theoretical basis for BL-99 and FOS synbiotics to improve constipation by regulating the gut microbiota and metabolites.
Clinical characteristics and immunotherapy response in paraneoplastic neurologic syndrome patients with increased number of high-risk antibodies
Authors: Wang Gong , Chen Mao , Gao Fei , Guo Meng , Li Maohua , He Qian , Jiang Jiaojin , Huang Cheng , Chen Xiaoyan , Xu Rui
Published: January 2025

Objective

To investigate the differences of clinical characteristics and treatment outcomes between paraneoplastic neurologic syndrome (PNS) patients with one high-risk antibody and patients with two high-risk antibodies.

Methods

We retrospectively analyzed the data of 51 PNS patients with high-risk antibody. Clinical data were extracted from the patients’ electronic medical records. Clinical presentations, cerebrospinal fluid (CSF) parameters, radiological characteristics and treatment outcomes between patients with one high-risk antibody and patients with two high-risk antibodies were analyzed.

Results

41 patients with 1 high-risk antibody and 10 patients with 2 high-risk antibodies were enrolled in this study. It was found that psychobehavioral abnormality (OR = 11.327, 95% CI: 1.371 to 93.602, P = 0.024), bowel and bladder dysfunction (OR = 23.537, 95% CI: 1.753 to 316.005, P = 0.017), and total protein of CSF (OR = 61.556, 95% CI: 2.926 to 1294.974, P = 0.008) were risk factors for increased number of high-risk antibodies in PNS. After immunotherapy treatment, Expanded Disability Status Scale (EDSS) scores in PNS patients with 2 high-risk antibodies were higher than that in PNS patients with 1 high-risk antibody (4.8 ± 2.4 vs. 3.0 ± 2.4, p = 0.043). EDSS change analysis also revealed that average EDSS score decreased after treatment in PNS with 1 Ab group while increased in PNS with 2 Abs group (p = 0.032).

Conclusions

Psychobehavioral abnormality, bowel and bladder dysfunction, and total protein of CSF were three variables associated with increased number of high-risk antibodies in PNS patients, while increased number of high-risk antibodies might indicate a poor immunotherapy response. Our findings might help to understand the association of PNS patients’ clinical features and high-risk antibodies, as well as to guide clinical practice.
Combining single-cell analysis and molecular docking techniques to construct a prognostic model for colon adenocarcinoma and uncovering inhibin subunit βb as a novel therapeutic target
Authors: Wu Qinqing , Ye Lu , Wu Yuwei , Zhao Mengyu , Lu Jiaxin , Yu Yanping , Niu Yixiao , Zhang Luxiao , Zuo Peijun
Published: January 2025

Background

Colon adenocarcinoma (COAD) is a malignancy with a high mortality rate and complex biological characteristics and heterogeneity, which poses challenges for clinical treatment. Anoikis is a type of programmed cell death that occurs when cells lose their attachment to the extracellular matrix (ECM), and it plays a crucial role in tumor metastasis. However, the specific biological link between anoikis and COAD, as well as its mechanisms in tumor progression, remains unclear, making it a potential new direction for therapeutic strategy research.

Methods

We employed transcriptomic data and clinical information from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to pinpoint differentially expressed anoikis-related genes (ARGs) in COAD. Using Cox proportional hazards models and Lasso regression analysis, we developed a prognostic signature derived from these ARGs. We also investigated the roles and interactions of these genes in the tumor microenvironment by analyzing single-cell RNA sequencing data. Additionally, we employed molecular docking techniques to evaluate the potential of inhibin subunit beta B (INHBB) as therapeutic targets and to assess the binding affinity of candidate drugs. Finally, we used gene knockout techniques to silence the key gene INHBB and explored its biological functions in vitro.

Results

In our study, by analyzing the expression differences of ARGs, we successfully classified patients with COAD. Kaplan-Meier survival analysis demonstrated that patients with elevated risk scores experienced poorer prognosis, a finding that was confirmed in both the training and validation cohorts. Additionally, immune infiltration analysis revealed a notable increase in immune cell presence within the tumor microenvironment of high-risk patients. Molecular docking identified potential drug candidates with high binding affinity to INHBB, including risperidone. Furthermore, in vitro experiments with INHBB showed that downregulation of its expression in COAD cell lines significantly reduced cellular viability and migration capacity.

Conclusion

In summary, our research, based on the expression characteristics of ARGs, provides new insights into the precise classification, prognosis assessment, and identification of potential therapeutic targets in COAD. It also validates the key role of INHBB in the progression of COAD, establishing the foundation for future personalized treatment strategies.
Technological progress bias and its impact on resource efficiency in China’s mariculture industry
Authors: Kong Jiaojiao , Sun Yanan
Published: January 2025

Technological progress (TC) is an important driving force of resource efficiency, and its bias has an important impact on resource efficiency. Based on the data of China’s mariculture industry from 2008 to 2020, this paper constructs a double-layer nested CES production function, and uses the seemingly unrelated regression method to estimate the elasticity of substitution between resource elements and non-resource elements of mariculture industry, and measures the level of resource biased technological progress (RBTC). On this basis, the vector autoregressive model is used to explore the relationship between RBTC, resource price and resource efficiency. The results show that: Firstly, there is complementarity between the resource elements of China’s mariculture industry and the non-resource elements aggregated by labor and capital. Secondly, there is a long-term equilibrium relationship between resource biased technological progress, resource price and resource efficiency. resource biased technological progress has a short-term negative and long-term positive impact on resource efficiency, and resource price has a short-term negative and long-term positive impact on resource efficiency. Based on this, this paper puts forward relevant policy recommendations to promote the improvement of resource efficiency of mariculture.
Demographic features, clinical characteristics, and comorbid relation in hidradenitis suppurativa: a population-based study
Authors: Kaya Gökhan , Özgen Fatma Pelin , Kelahmetoğlu Osman , Su Küçük Özlem , Onsun Nahide
Published: January 2025

Background/objective

Hidradenitis suppurativa (HS) is a chronic inflammatory disease affecting apocrine gland areas, characterized by painful nodules and abscesses that may result in sinus tracts and scarring. The global prevalence of HS is increasing due to heightened awareness, improved diagnostic methods, rising obesity rates, and higher smoking prevalence. This study aimed to describe the epidemiological, clinical, and comorbid characteristics of HS patients.

Methodology

This retrospective descriptive cross-sectional study included 193 outpatients aged between 15 and 73 years who visited a tertiary HS clinic between 2017 and 2022. Demographic, clinical, and comorbid characteristics were recorded and analyzed using chi-square and ordinal regression methods.

Results

The mean age was 34.5 ± 12.1 years, with a mean disease duration of 5.9 ± 6.7 years. According to the Hurley classification, 61.1, 24.4, and 14.5% were stages I, II, and III, respectively. Comorbidities were present in 48.2% of patients, with psychiatric disorders (19.2%), diabetes mellitus (14%), and hypertension (9.3%) being most common. Disease duration, smoking, male gender, and atypical localization were associated with increased disease severity.

Conclusion

Geographic and cultural factors influence the prevalence, severity, and management of HS, necessitating tailored treatment. Effective management requires multidisciplinary screening for early detection and prevention of comorbidities, including psychiatric disorders, cardiovascular conditions, and metabolic syndrome.
Influential articles in autism and gut microbiota: bibliometric profile and research trends
Authors: Ying Jiangbo , Zhang Melvyn Weibin , Wei Ker-Chiah , Wong Sunny H. , Subramaniam Mythily
Published: January 2025

Objective

Autism spectrum disorder (ASD) is a common neurodevelopmental disorder. Increasing evidence suggests that it is potentially related to gut microbiota, but no prior bibliometric analysis has been performed to explore the most influential works in the relationships between ASD and gut microbiota. In this study, we conducted an in-depth analysis of the most-cited articles in this field, aiming to provide insights to the existing body of research and guide future directions.

Methods

A search strategy was constructed and conducted in the Web of Science database to identify the 100 most-cited papers in ASD and gut microbiota. The Biblioshiny package in R was used to analyze and visualize the relevant information, including citation counts, country distributions, authors, journals, and thematic analysis. Correlation and comparison analyses were performed using SPSS software.

Results

The top 100 influential manuscripts were published between 2000 and 2021, with a total citation of 40,662. The average number of citations annually increased over the years and was significantly correlated to the year of publication (r = 0.481, p < 0.01, Spearman’s rho test). The United States was involved in the highest number of publications (n = 42). The number of publications in the journal was not significantly related to the journal’s latest impact factor (r = 0.016, p > 0.05, Spearman’s rho test). Co-occurrence network and thematic analysis identified several important areas, such as microbial metabolites of short-chain fatty acids and overlaps with irritable bowel syndrome.

Conclusion

This bibliometric analysis provides the key information of the most influential studies in the area of ASD and gut microbiota, and suggests the hot topics and future directions. The findings of this study can serve as a valuable reference for researchers and policymakers, guiding the development and implementation of the scientific research strategies in this area.
Phylogeography and biological characterization of H12N2 virus isolated from whooper swan in Central China
Authors: Ren Pengfei , Gao Zhen , Li Xing , Tang Jiao , Li Pei , Huang Zhonglin , Guo Jinchi , Cui Pengfei , Jin Lin , Li Junping , Liang Libin
Published: January 2025

Wild birds and waterfowl serve as the natural reservoirs of avian influenza viruses (AIVs). When AIVs originating from wild birds cross species barriers to infect mammals or humans, they pose a significant threat to public health. The H12 subtype of AIVs primarily circulates in wild birds, with relatively few isolates reported worldwide, and the evolutionary and biological characteristics of H12 subtype AIVs remain largely unknown. In this study, we analyzed the spatiotemporal distribution of H12 subtype AIVs worldwide and conducted a comprehensive investigation into the evolutionary and biological characteristics of an H12N2 virus isolated from a whooper swan in Central China. Phylogenetic analysis revealed that the H12N2 isolate belongs to the Eurasian lineage, with its HA gene likely originating from a duck-derived H12N5 virus and its NA gene potentially derived from an H9N2 virus, indicating that it is a complex reassorted virus. Animal experiments in domestic ducks and chickens demonstrated that the virus replicates at low levels in the respiratory tract of poultry and exhibits moderate horizontal transmission in ducks. However, it is capable of efficient horizontal transmission in chickens. Mouse infection experiments revealed that the virus could be detected in the nasal turbinates and lungs of mice, indicating that the H12N2 virus can infect mice without prior adaptation. In vitro studies revealed that the virus replicates efficiently in MDCK cells, with significantly higher titers than those in DF1 cells. These findings, combined with the mouse infection results, suggest that the H12N2 virus poses a potential risk of mammalian infection. This study provides valuable insights regarding the characteristics of the H12N2 virus and highlights the importance of ongoing surveillance and risk assessment of AIVs originating from wild birds.