bluuebunny/crossref_metadata_2025 huggingface dataset:
Relating Multi-Adjoint Normal Logic Programs to Core Fuzzy Answer Set Programs from a Semantical Approach
Authors: Cornejo M. Eugenia , Lobo David , Medina Jesús
Published: June 2020

This paper relates two interesting paradigms in fuzzy logic programming from a semantical approach: core fuzzy answer set programming and multi-adjoint normal logic programming. Specifically, it is shown how core fuzzy answer set programs can be translated into multi-adjoint normal logic programs and vice versa, preserving the semantics of the starting program. This translation allows us to combine the expressiveness of multi-adjoint normal logic programming with the compactness and simplicity of the core fuzzy answer set programming language. As a consequence, theoretical properties and results which relate the answer sets to the stable models of the respective logic programming frameworks are obtained. Among others, this study enables the application of the existence theorem of stable models developed for multi-adjoint normal logic programs to ensure the existence of answer sets in core fuzzy answer set programs.
A Method of Generating Fuzzy Implications from n Increasing Functions and n + 1 Negations
Authors: Rapti Maria N. , Papadopoulos Basil K.
Published: June 2020

In this paper, we introduce a new construction method of a fuzzy implication from n increasing functions g i : [ 0 , 1 ] → [ 0 , ∞ ) ,   ( g ( 0 ) = 0 ) ( i = 1 , 2 , … , n ,   n   ∈ ℕ ) and n + 1 fuzzy negations N i ( i = 1 , 2 , … , n + 1 ,   n   ∈ ℕ ). Imagine that there are plenty of combinations between n increasing functions g i and n + 1 fuzzy negations N i in order to produce new fuzzy implications. This method allows us to use at least two fuzzy negations N i and one increasing function g in order to generate a new fuzzy implication. Choosing the appropriate negations, we can prove that some basic properties such as the exchange principle (EP), the ordering property (OP), and the law of contraposition with respect to N are satisfied. The worth of generating new implications is valuable in the sciences such as artificial intelligence and robotics. In this paper, we have found a novel method of generating families of implications. Therefore, we would like to believe that we have added to the literature one more source from which we could choose the most appropriate implication concerning a specific application. It should be emphasized that this production is based on a generalization of an important form of Yager’s implications.
Metric f-Contact Manifolds Satisfying the (κ, μ)-Nullity Condition
Authors: Carriazo Alfonso , Fernández Luis M. , Loiudice Eugenia
Published: June 2020

We prove that if the f-sectional curvature at any point of a ( 2 n + s ) -dimensional metric f-contact manifold satisfying the ( κ , μ ) nullity condition with n > 1 is independent of the f-section at the point, then it is constant on the manifold. Moreover, we also prove that a non-normal metric f-contact manifold satisfying the ( κ , μ ) nullity condition is of constant f-sectional curvature if and only if μ = κ + 1 and we give an explicit expression for the curvature tensor field in such a case. Finally, we present some examples.
A Framework for Sharing Staff between Outbound and Inbound Airport Processes
Authors: Alodhaibi Sultan , Burdett Robert L. , Yarlagadda Prasad K.D.V.
Published: June 2020

This paper proposes an advanced simulation-optimization approach to evaluate and optimize the passenger flows within international airports. This approach allocates resources intelligently during the simulation process and balances demand and service quality. The resource allocation performed by our Advanced Resource Management (ARM) algorithm was used to develop an integrated system for arranging resources, identifying the proper resources, and allocating them throughout the model. It was used to investigate the influences of different staff allocation techniques on the inbound and outbound processes of an airport terminal. The purpose of the proposed simulation-optimization approach is to enhance passenger satisfaction through ensuring reasonable wait times during processing at the lowest cost possible (minimal staff hours).
Pyridine-2,6-Dithiocarboxylic Acid and Its Metal Complexes: New Inhibitors of New Delhi Metallo -Lactamase-1
Authors: Thomas Chris S. , Braun Doug R. , Olmos Jose Luis , Rajski Scott R. , Phillips George N. , Andes David , Bugni Tim S.
Published: June 2020

Carbapenem-resistant Enterobacteriaceae continue to threaten human health worldwide with few effective treatment options. New Delhi metallo--lactamase (NDM) enzymes are a contributing element that drive resistance to many -lactam- and carbapenem-based antimicrobials. Many NDM inhibitors are known, yet none are clinically viable. In this study, we present and characterize a new class of NDM-1 inhibitors based on a pyridine-2,6-dithiocarboxylic acid metal complex scaffold. These complexes display varied and unique activity profiles against NDM-1 in kinetic assays and serve to increase the effectiveness of meropenem, an established antibacterial, in assays using clinical Enterobacteriaceae isolates.
A Review of Challenges and Opportunities Associated with Bolted Flange Connections in the Offshore Wind Industry
Authors: Mehmanparast Ali , Lotfian Saeid , Vipin Sukumara Pillai
Published: June 2020

The use of bolted flange connections in the offshore wind industry has steeply risen in the last few years. This trend is because of failings observed in other modes of joints such as grouted joints, coupled with enormous economic losses associated with such failures. As many aspects of bolted flange connections for the offshore wind industry are yet to be understood in full, the current study undertakes a comprehensive review of the lessons learned about bolted connections from a range of industries such as nuclear, aerospace, and onshore wind for application in offshore wind industry. Subsequently, the collected information could be used to effectively address and investigate ways to improve bolted flange connections in the offshore wind industry. As monopiles constitute an overwhelming majority of foundation types used in the current offshore wind market, this work focusses on large diameter flanges in the primary load path of a wind turbine foundation, such as those typically found at the base of turbine towers, or at monopile to transition piece connections. Finally, a summary of issues associated with flanges as well as bolted connections is provided, and insights are recommended on the direction to be followed to address these concerns.
Corrosion-Fatigue Analysis of High-Strength Steel Wire by Experiment and the Numerical Simulation
Authors: Xue Songling , Shen Ruili
Published: June 2020

The paper takes the corrosion fatigue damage of cable or sling in the actual bridge as a starting point. The high-strength steel wire is chosen as the basic component to study the corrosion fatigue failure mode. The service life prediction model is put forward, which provides a basis for future research. In this paper, the S-N curves of the steel wire with the different corrosion degrees are given through fatigue tests of six groups of steel wire under different corrosion conditions. The results show that the higher the corrosion degree, the steeper the S-N curve, and the fatigue life considering corrosion are much lower than that without considering corrosion. Finally, a fatigue life prediction model considering the coupling effect of corrosion fatigue is proposed and embedded into Abaqus v6.14 (Dassault, Paris, French). The calculation results show that the fatigue model considering the corrosion can predict the service life to some extent.
Microstructure and Electrical Property of Ex-Situ and In-Situ Copper Titanium Carbide Nanocomposites
Authors: Viet Nguyen Hoang , Oanh Nguyen Thi Hoang
Published: June 2020

In this study, ex-situ Cu-TiC nanocomposites of 1, 3 and 5 vol. % TiC and in-situ Cu-TiH2-C nanocomposites (corresponding to 5 vol. % TiC) were prepared using ball milling and spark plasma sintering methods. Powder mixtures were milled for 4 h at 400 rpm. As-milled Cu-TiC composite powders were consolidated under an applied pressure of 70 MPa. The phase composition, and microstructure of the composite samples were characterized by X-ray diffraction, and scanning electron microscope and transmission electron microscope techniques, respectively. With the increasing TiC content from 1 to 5 vol. %, the hardness of the ex-situ composites when sintered at 600 °C changed between 161.4 and 178.5 HV and the electrical conductivity decreased from 52.1 to 47.6% IACS. In-situ Cu-TiH2-C nanocomposite sintered at 950 °C had higher hardness and electrical conductivity than ex-situ Cu-TiC composite due to having a homogenous distribution of nano reinforcement particles and dense structure.
Microstructural Evolution and Mechanical Properties of Graphene-Reinforced Ti-6Al-4V Composites Synthesized via Spark Plasma Sintering
Authors: Wang Wei , Zhou Haixiong , Wang Qingjuan , Wei Baojia , Xin Shewei , Gao Yuan
Published: June 2020

Ti-6Al-4V alloy (TC4) with different concentrations of graphene nanoplatelets (GNPs) were fabricated by ball milling and spark plasma sintering (SPS). Microstructure characteristics of the composites were characterized by X-Ray Diffraction (XRD), Scanning electron microscopy (SEM), and Raman. Microhardness and the compressive mechanical properties were also investigated. Experimental results showed that in the process of SPS, most of the GNPs were still retained at high pressure and temperature, and a new phase of TiC was presented due to the in-situ reaction between TiC and GNPs. Also, the strength of the composites was depended on the concentration of GNPs in TC4 matrix. Consequently, the composite with 0.8 wt. % GNPs was increased 18% in microhardness. The maximum yield strength and ductility of the composite were increased by 22.2% and 43.2%, respectively. The strengthening mechanism of the composites was further discussed, and the Orowan strengthening mechanism was the main strengthening factor.
Heterologous Production of 6-Deoxyerythronolide B in Escherichia coli through the Wood Werkman Cycle
Authors: Gonzalez-Garcia R. Axayacatl , Nielsen Lars K. , Marcellin Esteban
Published: June 2020

Polyketides are a remarkable class of natural products with diverse functional and structural diversity. The class includes many medicinally important molecules with antiviral, antimicrobial, antifungal and anticancer properties. Native bacterial, fungal and plant hosts are often difficult to cultivate and coax into producing the desired product. As a result, Escherichia coli has been used for the heterologous production of polyketides, with the production of 6-deoxyerythronolide B (6-dEB) being the first example. Current strategies for production in E. coli require feeding of exogenous propionate as a source for the precursors propionyl-CoA and S-methylmalonyl-CoA. Here, we show that heterologous polyketide production is possible from glucose as the sole carbon source. The heterologous expression of eight genes from the Wood-Werkman cycle found in Propionibacteria, in combination with expression of the 6-dEB synthases DEBS1, DEBS2 and DEBS3 resulted in 6-dEB formation from glucose as the sole carbon source. Our results show that the Wood-Werkman cycle provides the required propionyl-CoA and the extender unit S-methylmalonyl-CoA to produce up to 0.81 mg/L of 6-dEB in a chemically defined media.
Cornell’s Role in Developing Synchrotron Radiation for Mineral Physics
Authors: Bassett William A.
Published: June 2020

The newly invented diamond anvil cell (DAC) in 1960, and the newly constructed Cornell High Energy Synchrotron Source (CHESS) in 1979 were a perfect match, as CHESS could provide such an intense X-ray beam with such extraordinary properties that a whole new approach to mineral physics research became possible. The very high intensity of the X-ray beam from CHESS made it possible to make real-time observations of crystal structures during phase transitions for the first time. For instance, the olivine-spinel transition, important for understanding deep focus earthquakes can be shown to take place first by the displacive shift of oxygen layers supporting shear stress as most likely earthquake trigger followed by the diffusion of the cations to their positions in the spinel structure. X-ray emission spectra of high-pressure, high-temperature samples also made it possible to determine phase compositions, as well as the structures of complex ions in solution.
Carbon Nanomaterials for Sorption of 68Ga for Potential Using in Positron Emission Tomography
Authors: Kazakov Andrey G. , Garashchenko Bogdan L. , Ivanova Milana K. , Vinokurov Sergey E. , Myasoedov Boris F.
Published: June 2020

In present work, carbon nanomaterials (CNMs) are investigated as potential carriers of 68Ga, which is widely used in positron emission tomography (PET) in nuclear medicine. Sorption behavior of 68Ga was studied onto CNMs of various structures and chemical compositions: nanodiamonds (ND), reduced graphite oxide (rGiO) and multi-walled carbon nanotubes (MWCNT), as well as their oxidized (ND–COOH) or reduced (rGiO–H, MWCNT–H) forms. The physicochemical properties of the nanoparticles were determined by high resolution transmission electron microscopy, x-ray photoelectron spectroscopy, dynamic light scattering and potentiometric titration. The content of 68Ga in the solutions during the study of sorption was determined by gamma-ray spectrometry. The highest degree of 68Ga sorption was observed on ND and ND–COOH samples, and the optimal sorption conditions were determined: an aqueous solution with a pH of 5–7, m/V ratio of 50 μg/mL and a room temperature (25 °C). The 68Ga@ND and 68Ga@ND–COOH conjugates were found to be stable in a model blood solution—phosphate-buffered saline with a pH of 7.3, containing 40 g/L of bovine serum albumin: 68Ga desorption from these samples in 90 minutes was no more than 20% at 25 °C and up to 30% at 37 °C. Such a quantity of desorbed 68Ga does not harm the body and does not interfere with the PET imaging process. Thus, ND and ND–COOH are promising CNMs for using as carriers of 68Ga for PET diagnostics.
Deriving Optimized PID Parameters of Nano-Ag Colloid Prepared by Electrical Spark Discharge Method
Authors: Tseng Kuo-Hsiung , Lin Yur-Shan , Lin Yun-Chung , Tien Der-Chi , Stobinski Leszek
Published: June 2020

Using the electrical spark discharge method, this study prepared a nano-Ag colloid using self-developed, microelectrical discharge machining equipment. Requiring no additional surfactant, the approach in question can be used at the ambient temperature and pressure. Moreover, this novel physical method of preparation produced no chemical pollution. This study conducted an in-depth investigation to establish the following electrical discharge conditions: gap electrical discharge, short circuits, and open circuits. Short circuits affect system lifespan and cause electrode consumption, resulting in large, non-nanoscale particles. Accordingly, in this study, research for and design of a new logic judgment circuit set was used to determine the short-circuit rate. The Ziegler–Nichols proportional–integral–derivative (PID) method was then adopted to find optimal PID values for reducing the ratio between short-circuit and discharge rates of the system. The particle size, zeta potential, and ultraviolet spectrum of the nano-Ag colloid prepared using the aforementioned method were also analyzed with nanoanalysis equipment. Lastly, the characteristics of nanosized particles were analyzed with a transmission electron microscope. This study found that the lowest ratio between short-circuit rates was obtained (1.77%) when PID parameters were such that Kp was 0.96, Ki was 5.760576, and Kd was 0.039996. For the nano-Ag colloid prepared using the aforementioned PID parameters, the particle size was 3.409 nm, zeta potential was approximately −46.8 mV, absorbance was approximately 0.26, and surface plasmon resonance was 390 nm. Therefore, this study demonstrated that reducing the short-circuit rate can substantially enhance the effectiveness of the preparation and produce an optimal nano-Ag colloid.
Antibacterial and Osteogenic Activity of Titania Nanotubes Modified with Electrospray-Deposited Tetracycline Nanoparticles
Authors: Im Su-Yeon , Kim Kwang-Mahn , Kwon Jae-Sung
Published: June 2020

The nanotubular surface of titanium implants is known to have superior osteogenic activity but is also vulnerable to failure because of induced bacterial attachment and consequent secondary infection. Here, the problem was attempted to be solved by depositing nanosized tetracycline (TC)-loaded particles in poly(lactic-co-glycolic acid) on titania nanotubes (TNTs) using the electrospray deposition method. The antibacterial effect of the newly formed TNT surface was considered using the common pathogen Staphylococcus aureus. Maintenance of the biocompatibility and osteogenic characteristics of TNTs has been tested through cytotoxicity tests and osteogenic gene expression/extra-cellular matrix mineralization, respectively. The results showed that TNTs were successfully formed by anodization, and the characterization of TC deposited on the TNTs was controlled by varying the spraying parameters such as particle size and coating time. The TC nanoparticle-coated TNTs showed antibacterial activity against Staphylococcus aureus and biocompatibility with MC3T3-E1 pre-osteoblasts, while the osteogenic activity of the TNT structure was preserved, as demonstrated by osteocalcin and osteopontin gene expression, as well as Alizarin red staining. Hence, this study concluded that the electrosprayed TC coating of TNTs is a simple and effective method for the formation of bactericidal implants that can maintain osteogenic activity.
The Improvement of Nanoemulsion Stability and Antioxidation via Protein-Chlorogenic Acid-Dextran Conjugates as Emulsifiers
Authors: Liu Chang , Jin Hua , Yu Yue , Sun Jingying , Zheng Huanyu , Zhang Yan , Xu Jing , Zhu Xiuqing
Published: June 2020

In this experiment, the peanut protein isolate (PPI), soybean protein isolate (SPI), rice bran protein isolate (RBPI), and whey protein isolate (WPI) were modified by linking chlorogenic acid covalently and linking dextran by Maillard reaction to prepare protein-chlorogenic acid-dextran (PCD) conjugates. As for structures, conformational changes of conjugates were determined by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE), Fourier transform infrared (FT-IR), and fluorescence measurements. The molecular weights of PCD conjugates became larger, the structure became disorder, and the amino acid residues inside the protein were exposed to the polar environment when compared to protein-chlorogenic acid (PC) and native proteins (NPs). As for properties, the interfacial tension reduced and antioxidant activity of PCD conjugates enhanced in varying degrees. Based on this, PCD conjugates were used as emulsifiers in order to investigate the properties of nanoemulsions and compared with PC conjugates and NPs. The mean droplet diameters (MDD) results showed that the nanoemulsions that were stabilized by PCD conjugates had the smallest particle sizes and exhibited uniformly dispersed spherical shapes. The storage and oxidative stabilities of PCD conjugates were also significantly improved. In comparison, nanoemulsion that was stabilized by PPI-chlorogenic acid-dextran conjugate had the smallest particle size and optimal stability among four protein stabilized nanoemulsions.
Controllable Carbonization of Plastic Waste into Three-Dimensional Porous Carbon Nanosheets by Combined Catalyst for High Performance Capacitor
Authors: Mu Xueying , Li Yunhui , Liu Xiaoguang , Ma Changde , Jiang Hanqing , Zhu Jiayi , Chen Xuecheng , Tang Tao , Mijowska Ewa
Published: June 2020

Polyethylene terephthalate (PET) plastic has been extensively used in our social life, but its poor biodegradability has led to serious environmental pollution and aroused worldwide concern. Up to now, various strategies have been proposed to address the issue, yet such strategies remain seriously impeded by many obstacles. Herein, waste PET plastic was selectively carbonized into three-dimensional (3D) porous carbon nanosheets (PCS) with high yield of 36.4 wt%, to be further hybridized with MnO2 nanoflakes to form PCS-MnO2 composites. Due to the introduction of an appropriate amount of MnO2 nanoflakes, the resulting PCS-MnO2 composite exhibited a specific capacitance of 210.5 F g−1 as well as a high areal capacitance of 0.33 F m−2. Furthermore, the PCS-MnO2 composite also showed excellent cycle stability (90.1% capacitance retention over 5000 cycles under a current density of 10 A g−1). The present study paved an avenue for the highly efficient recycling of PET waste into high value-added products (PCSs) for electrochemical energy storage.
Thermal Performance of Hybrid-Inspired Coolant for Radiator Application
Authors: Benedict F. , Kumar Amit , Kadirgama K. , Mohammed Hussein A. , Ramasamy D. , Samykano M. , Saidur R.
Published: June 2020

Due to the increasing demand in industrial application, nanofluids have attracted the considerable attention of researchers in recent decades. The addition of nanocellulose (CNC) with water (W) and ethylene glycol (EG) to a coolant for a radiator application exhibits beneficial properties to improve the efficiency of the radiator. The focus of the present work was to investigate the performance of mono or hybrid metal oxide such as Al2O3 and TiO2 with or without plant base-extracted CNC with varying concentrations as a better heat transfer nanofluid in comparison to distilled water as a radiator coolant. The CNC is dispersed in the base fluid of EG and W with a 60:40 ratio. The highest absorption peak was noticed at 0.9% volume concentration of TiO2, Al2O3, CNC, Al2O3/TiO2, and Al2O3/CNC nanofluids which indicates a better stability of the nanofluids’ suspension. Better thermal conductivity improvement was observed for the Al2O3 nanofluids in all mono nanofluids followed by the CNC and TiO2 nanofluids, respectively. The thermal conductivity of the Al2O3/CNC hybrid nanofluids with 0.9% volume concentration was found to be superior than that of the Al2O3/TiO2 hybrid nanofluids. Al2O3/CNC hybrid nanofluid dominates over other mono and hybrid nanofluids in terms of viscosity at all volume concentrations. CNC nanofluids (all volume concentrations) exhibited the highest specific heat capacity than other mono nanofluids. Additionally, in both hybrid nanofluids, Al2O3/CNC showed the lowest specific heat capacity. The optimized volume concentration from the statistical analytical tool was found to be 0.5%. The experimental results show that the heat transfer coefficient, convective heat transfer, Reynolds number and the Nusselt number have a proportional relationship with the volumetric flow rate. Hybrid nanofluids exhibit better thermal conductivity than mono nanofluids. For instance, a better thermal conductivity improvement was shown by the mono Al2O3 nanofluids than the CNC and TiO2 nanofluids. On the other hand, superior thermal conductivity was observed for the Al2O3/CNC hybrid nanofluids compared to the other mono and hybrid ones (Al2O3/TiO2).
Difensil Immuno Reduces Recurrence and Severity of Tonsillitis in Children: A Randomized Controlled Trial
Authors: Di Stadio Arianna , della Volpe Antonio , Korsch Fiammetta M. , De Lucia Antonietta , Ralli Massimo , Martines Francesco , Ricci Giampietro
Published: June 2020

Oral supplements (OS) support the immune system in fighting upper airways infection. This study aimed to analyze the effect of Difensil Immuno (DI) on the recurrence of tonsillitis and fever in children. A multicentric randomized clinical trial was conducted. One-hundred and twenty children with chronic tonsillitis were randomly assigned to group A, B or control. Patients in group A were treated with 10 mL of DI for 90 consecutive days, patients in group B underwent treatment with 15 mL of DI for 45 consecutive days. The following data were collected at baseline (T0), T1 and T2: tonsillitis and fever episodes, tonsillar volume, blood test results. One-way ANOVA was used to analyze within and between variances. Patients in group A and B statistically improved their clinical parameters (episode of tonsillitis and fever, tonsillar volume) when compared to control group both at T1 and T2. However, T1 variances were more consistent in group A than in group B. All patients in the study groups improved their clinical outcomes. No statistically significant variances were observed in blood parameters both at T1 and T2. Our results suggest that children treated with DI had fewer episodes of tonsillitis and fever and a reduction in their tonsillar volume.
Pathophysiological Mechanisms by which Heat Stress Potentially Induces Kidney Inflammation and Chronic Kidney Disease in Sugarcane Workers
Authors: Hansson Erik , Glaser Jason , Jakobsson Kristina , Weiss Ilana , Wesseling Catarina , Lucas Rebekah A. I. , Wei Jason Lee Kai , Ekström Ulf , Wijkström Julia , Bodin Theo , Johnson Richard J. , Wegman David H.
Published: June 2020

Background: Chronic kidney disease of non-traditional origin (CKDnt) is common among Mesoamerican sugarcane workers. Recurrent heat stress and dehydration is a leading hypothesis. Evidence indicate a key role of inflammation. Methods: Starting in sports and heat pathophysiology literature, we develop a theoretical framework of how strenuous work in heat could induce kidney inflammation. We describe the release of pro-inflammatory substances from a leaky gut and/or injured muscle, alone or in combination with tubular fructose and uric acid, aggravation by reduced renal blood flow and increased tubular metabolic demands. Then, we analyze longitudinal data from >800 sugarcane cutters followed across harvest and review the CKDnt literature to assess empirical support of the theoretical framework. Results: Inflammation (CRP elevation and fever) and hyperuricemia was tightly linked to kidney injury. Rehydrating with sugary liquids and NSAID intake increased the risk of kidney injury, whereas electrolyte solution consumption was protective. Hypokalemia and hypomagnesemia were associated with kidney injury. Discussion: Heat stress, muscle injury, reduced renal blood flow and fructose metabolism may induce kidney inflammation, the successful resolution of which may be impaired by daily repeating pro-inflammatory triggers. We outline further descriptive, experimental and intervention studies addressing the factors identified in this study.
Design and Characterization of Spray-Dried Chitosan-Naltrexone Microspheres for Microneedle-Assisted Transdermal Delivery
Authors: Ogunjimi Abayomi T. , Fiegel Jennifer , Brogden Nicole K.
Published: June 2020

Naltrexone (NTX) hydrochloride is a potent opioid antagonist with significant first-pass metabolism and notable untoward effects when administered orally or intramuscularly. Microneedle (MN)-assisted transdermal delivery is an attractive alternative that can improve therapeutic delivery to deeper skin layers. In this study, chitosan-NTX microspheres were developed via spray-drying, and their potential for transdermal NTX delivery in association with MN skin treatment was assessed. A quality-by-design approach was used to evaluate the impact of key input variables (chitosan molecular weight, concentration, chitosan-NTX ratio, and feed flow rate) on microsphere physical characteristics, encapsulation efficiency, and drug-loading capacity. Formulated microspheres had high encapsulation efficiencies (70–87%), with drug-loading capacities ranging from 10–43%. NTX flux through MN-treated skin was 11.6 ± 2.2 µg/cm2·h from chitosan-NTX microspheres, which was significantly higher than flux across intact skin. Combining MN-assisted delivery with the chitosan microsphere formulation enabled NTX delivery across the skin barrier, while controlling the dose released to the skin.
Development and Characterization of Liquisolid Tablets Based on Mesoporous Clays or Silicas for Improving Glyburide Dissolution
Authors: Cirri Marzia , Mura Paola , Valleri Maurizio , Brunetti Letizia
Published: June 2020

The aim of this work was to evaluate the effectiveness of mesoporous clays or silicas to develop fast-dissolving glyburide tablets based on a liquisolid approach. Selected clay (Neusilin®US2) and silica (Aeroperl®300) allowed preparation of innovative drug liquisolid systems containing dimethylacetamide or 2-pyrrolidone as drug solvents, without using coating materials which are necessary in conventional systems. The obtained liquisolid powders were characterized for solid-state properties, flowability, compressibility, morphology, granulometry, and then used for directly compressed tablet preparation. The developed liquisolid tablets provided a marked drug dissolution increase, reaching 98% dissolved drug after 60 min, compared to 40% and 50% obtained from a reference tablet containing the plain drug, and a commercial tablet. The improved glyburide dissolution was attributed to its increased wetting properties and surface area, due to its amorphization/solubilization within the liquisolid matrix, as confirmed by DSC and PXRD studies. Mesoporous clay and silica, owing to their excellent adsorbent, flow, and compressibility properties, avoided use of coating materials and considerably improved liquid-loading capacity, reducing the carrier amount necessary to obtain freely flowing powders. Neusilin®US2 showed a superior performance than Aeroperl®300 in terms of the tablet’s technological properties. Finally, simplicity and cost-effectiveness of the proposed approach make it particularly advantageous for industrial scale-up.
Bitter Melon (Momordica charantia L.) Rootstock Improves the Heat Tolerance of Cucumber by Regulating Photosynthetic and Antioxidant Defense Pathways
Authors: Tao Mei-Qi , Jahan Mohammad Shah , Hou Kun , Shu Sheng , Wang Yu , Sun Jin , Guo Shi-Rong
Published: June 2020

High temperature is considered a critical abiotic stressor that is increasing continuously, which is severely affecting plant growth and development. The use of heat-resistant rootstock grafting is a viable technique that is practiced globally to improve plant resistance towards abiotic stresses. In this experiment, we explored the efficacy of bitter melon rootstock and how it regulates photosynthesis and the antioxidant defense system to alleviate heat stress (42 °C/32 °C) in cucumber. Our results revealed that bitter-melon-grafted seedlings significantly relieved heat-induced growth inhibition and photoinhibition, maintained better photosynthesis activity, and accumulated a greater biomass than self-grafted seedlings. We measured the endogenous polyamine and hydrogen peroxide (H2O2) contents to determine the inherent mechanism responsible for these effects, and the results showed that heat stress induced a transient increase in polyamines and H2O2 in the inner courtyard of grafted seedlings. This increment was greater and more robust in bitter-melon-grafted seedlings. In addition, the use of polyamine synthesis inhibitors MGBG (methylglyoxal bis-guanylhydrazone) and D-Arg (D-arginine), further confirmed that the production of H2O2 under heat stress is mediated by the accumulation of endogenous polyamines. Moreover, compared with other treatments, the bitter-melon-grafted seedlings maintained high levels of antioxidant enzyme activity under high temperature conditions. However, these activities were significantly inhibited by polyamine synthesis inhibitors and H2O2 scavengers (dimethylthiourea, DMTU), indicating that bitter melon rootstock not only maintained better photosynthetic activity under conditions of high temperature stress but also mediated the production of H2O2 through the regulation of the high level of endogenous polyamines, thereby boosting the antioxidant defense system and comprehensively improving the heat tolerance of cucumber seedlings. Taken together, these results indicate that grafting with a resistant cultivar is a promising alternative tool for reducing stress-induced damage.
Transcriptome Comparison of Defense Responses in the Rice Variety ‘Jao Hom Nin’ Regarding Two Blast Resistant Genes, Pish and Pik
Authors: Ngernmuen Athipat , Suktrakul Worrawit , Kate-Ngam Sureeporn , Jantasuriyarat Chatchawan
Published: June 2020

Jao Hom Nin (JHN) is a Thai rice variety with broad-spectrum resistant against rice blast fungus. JHN contains two rice blast resistant genes, Pish and Pik, located on chromosome 1 and on chromosome 11, respectively. To understand the blast resistance in JHN, the study of the defense mechanism related to the Pish and Pik genes is crucial. This study aimed to dissect defense response genes between the Pish and Pik genes using the RNA-seq technique. Differentially expressed genes (DEGs) of Pish and Pik backcross inbred lines were identified between 0 and 24 h after inoculation with rice blast spore suspension. The results showed that 1248 and 858 DEGs were unique to the Pish and Pik lines, respectively. The wall-associated kinase gene was unique to the Pish line and the zinc-finger-containing protein gene was unique to the Pik line. Pathogenicity-related proteins PR-4 and PR-10 were commonly found in both Pish and Pik lines. Moreover, DEGs functionally categorized in brassinosteriod, jasmonic acid, and salicylic acid pathways were detected in both Pish and Pik lines. These unique and shared genes in the Pish and Pik rice blast defense responses will help to dissect the mechanisms of plant defense and facilitate rice blast breeding programs.
Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
Authors: Wang Eu Sheng , Kieu Nam Phuong , Lenman Marit , Andreasson Erik
Published: June 2020

Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by Phytophthora infestans. Stacking of resistance (R) genes from wild Solanum relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato.
The Serine Carboxypeptidase-Like Gene SCPL41 Negatively Regulates Membrane Lipid Metabolism in Arabidopsis thaliana
Authors: Chen Juan , Li Wei-qi , Jia Yan-xia
Published: June 2020

The Arabidopsis has 51 proteins annotated as serine carboxypeptidase-like (SCPL) enzymes. Although biochemical and cellular characterization indicates SCPLs involved in protein turnover or processing, little is known about their roles in plant metabolism. In this study, we identified an Arabidopsis mutant, bis4 (1-butanol insensitive 4), that was insensitive to the inhibitory effect of 1-butanol on seed germination. We cloned the gene that was defective in bis4 and found that it encoded an SCPL41 protein. Transgenic Arabidopsis plants constitutively expressing SCPL41 were generated, oil body staining and lipidomic assays indicated that SCPL41-overexpressing plants showed a decrease in membrane lipid content, especially digalactosyl diglyceride (DGDG) and monogalactosyl diglyceride (MGDG) contents, while the loss of SCPL41 increased the membrane lipid levels compared with those in wild-type plants. These findings suggested that SCPL41 had acquired novel functions in membrane lipid metabolism.
Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration
Authors: Bidabadi Siamak Shirani , Jain S. Mohan
Published: June 2020

Plants generally have the highest regenerative ability because they show a high degree of developmental plasticity. Although the basic principles of plant regeneration date back many years, understanding the cellular, molecular, and physiological mechanisms based on these principles is currently in progress. In addition to the significant effects of some factors such as medium components, phytohormones, explant type, and light on the regeneration ability of an explant, recent reports evidence the involvement of molecular signals in organogenesis and embryogenesis responses to explant wounding, induced plant cell death, and phytohormones interaction. However, some cellular behaviors such as the occurrence of somaclonal variations and abnormalities during the in vitro plant regeneration process may be associated with adverse effects on the efficacy of plant regeneration. A review of past studies suggests that, in some cases, regeneration in plants involves the reprogramming of distinct somatic cells, while in others, it is induced by the activation of relatively undifferentiated cells in somatic tissues. However, this review covers the most important factors involved in the process of plant regeneration and discusses the mechanisms by which plants monitor this process.
Alkaline Hydrolysis and Dyeing Characteristics of Sea-Island-Type Ultramicrofibers of PET Tricot Fabrics with Black Disperse Dye
Authors: Kang Jeong Min , Kim Min Gu , Lee Ji Eun , Ko Jae Wang , Kim Il Jin , Lee Jae Yeon , Lee Dong Jin , Ko Seong Ik , Jung Dae Ho , Lee Seung Geol
Published: June 2020

In this study, we investigated conditions for the alkaline hydrolysis and black-disperse dyeing of sea-island-type polyethylene terephthalate (PET) ultramicrofiber tricot fabric. We examined the weight loss ratios and tensile strengths according to the NaOH content (10–30% on mass of fabric (omf)) during treatment; the optimal conditions used 25% omf NaOH for 30 min at 100 °C for an average weight loss ratio of 23.47%. By scanning electron microscope (SEM) analysis, the ‘sea’ components are extracted with increasing NaOH concentration until 25% omf NaOH, and damage of the ‘island’ components above 25% omf NaOH leads to a reduction in tensile strength. The dyeing conditions, including temperature (95–135 °C), time (20–60 min), pH buffer solution concentration (1–9 g/L), and contents of dispersant (1–9 g/L) and UV-absorbent (5–25% omf) were also explored. The optimal dyeing conditions were established as a dye concentration of 8% omf with 1 g/L dispersant, 1 g/L pH buffer solution concentration, and 10% omf UV-absorbent at 135 °C for 40 min at a 1:10 goods-to-liquor ratio. The rubbing colorfastness values for the fabrics dyed with the black disperse dye spanned four grades under dry and wet conditions. The light colorfastness values of the dyed fabrics were good to excellent in the range of 4–5 grades.
Enhancing the Mechanical and Tribological Properties of Cellulose Nanocomposites with Aluminum Nanoadditives
Authors: Shi Shih-Chen , Chen Tao-Hsing , Mandal Pramod Kumar
Published: June 2020

Hydroxypropyl methylcellulose (HPMC) is a common hydrophilic and biodegradable polymer that can form films. This study incorporated aluminum nanoadditives as an enhancement reagent into a HPMC matrix. Mechanical properties of nanocompoistes, including the tensile strength and the elastic modulus, were analyzed with a nano-tensile tester. The incorporation of additives in HPMC films significantly enhances their mechanical and film barrier properties. Evidence of bonding between the additive and matrix was observed by Fourier-transform infrared spectrometer analysis. The additives occupy the spaces in the pores of the matrix, which increases the tendency of the pore to collapse and improves the chemical bonding between the base material and the additives. The incorporation of excess additives decreases the tensile strength due to ineffective collisions between the additives and the matrix. The wear test proves that the addition of nano-additives can improve the tribology performance of the HPMC composite while reducing the wear volume and the friction. Bonding between the nanoadditives and the matrix does not help release the nanoadditives into the wear interface as a third-body layer. The main reason to enhance the tribology performance is that the nanoadditives improve the load-capacity of the composite coating. This hybrid composite can be useful in many sustainability applications.
Post-Polymerization Modification of Ring Opening Metathesis Polymerization (ROMP)-Derived Materials Using Wittig Reactions
Authors: Duty Ryan , Hobbs Christopher E.
Published: June 2020

This communication describes our recent efforts to utilize Wittig olefination reactions for the post-polymerization modification of polynorbornene derivatives prepared through ring opening metathesis polymerization (ROMP). Polymerizing α-bromo ester-containing norbornenes provides polymers that can undergo facile substitution with triphenylphosphine. The resulting polymeric phosphonium salt is then deprotonated to form an ylide that undergoes reaction with various aryl aldehydes in a one-pot fashion to yield the respective cinnamates. These materials can undergo further modification through photo-induced [2 + 2] cycloaddition cross-linking reactions.
Quantifying Oxygen Levels in 3D Bioprinted Cell-Laden Thick Constructs with Perfusable Microchannel Networks
Authors: Figueiredo Lara , Le Visage Catherine , Weiss Pierre , Yang Jing
Published: June 2020

The survival and function of thick tissue engineered implanted constructs depends on pre-existing, embedded, functional, vascular-like structures that are able to integrate with the host vasculature. Bioprinting was employed to build perfusable vascular-like networks within thick constructs. However, the improvement of oxygen transportation facilitated by these vascular-like networks was directly quantified. Using an optical fiber oxygen sensor, we measured the oxygen content at different positions within 3D bioprinted constructs with and without perfusable microchannel networks. Perfusion was found to play an essential role in maintaining relatively high oxygen content in cell-laden constructs and, consequently, high cell viability. The concentration of oxygen changes following switching on and off the perfusion. Oxygen concentration depletes quickly after pausing perfusion but recovers rapidly after resuming the perfusion. The quantification of oxygen levels within cell-laden hydrogel constructs could provide insight into channel network design and cellular responses.
Glycopolymer Brushes by Reversible Deactivation Radical Polymerization: Preparation, Applications, and Future Challenges
Authors: Ribeiro Jessica P. M. , Mendonça Patrícia V. , Coelho Jorge F. J. , Matyjaszewski Krzysztof , Serra Arménio C.
Published: June 2020

The cellular surface contains specific proteins, also known as lectins, that are carbohydrates receptors involved in different biological events, such as cell–cell adhesion, cell recognition and cell differentiation. The synthesis of well-defined polymers containing carbohydrate units, known as glycopolymers, by reversible deactivation radical polymerization (RDRP) methods allows the development of tailor-made materials with high affinity for lectins because of their multivalent interaction. These polymers are promising candidates for the biomedical field, namely as novel diagnostic disease markers, biosensors, or carriers for tumor-targeted therapy. Although linear glycopolymers are extensively studied for lectin recognition, branched glycopolymeric structures, such as polymer brushes can establish stronger interactions with lectins. This specific glycopolymer topology can be synthesized in a bottlebrush form or grafted to/from surfaces by using RDRP methods, allowing a precise control over molecular weight, grafting density, and brush thickness. Here, the preparation and application of glycopolymer brushes is critically discussed and future research directions on this topic are suggested.
Development and Evaluation of a Polyvinylalcohol -Cellulose Derivative-Based Film with Povidone-Iodine Predicted for Wound Treatment
Authors: Kida Dorota , Gładysz Olimpia , Szulc Małgorzata , Zborowski Jacek , Junka Adam , Janeczek Maciej , Lipińska Anna , Skalec Aleksandra , Karolewicz Bożena
Published: June 2020

The aim of this study was to develop and assess a polyvinyl alcohol-cellulose derivatives-based film with incorporated povidone-iodine (PVP-I) predicted for applications in the treatment of periodontitis. Films were fabricated by solvent-casting, and their physical characteristics, such as their surface and structure morphology, mechanical properties, and disintegrating time, were evaluated. For in vitro iodine release studies and evaluation, the antimicrobial activity was tested using a modified disc diffusion method against five microbial strains. For further use, we selected the film with polyvinyl alcohol-hydroxypropyl methylcellulose (PVA/HPMC_B) based on acceptable physicochemical properties. To assess the subacute toxicity of the film composition, the tissue regeneration process was tested in rats and compared to a conventional dressing commonly used in wound healing (Spongostan). Seven days after implantation, dorsal skin sections and blood samples (n = 10, in total n = 30) were examined. The wound area, epithelium, and dermis were evaluated microscopically, while the blood collected from the rats underwent biochemical analysis. The blood biochemistry results were comparable in all three groups. No significant histological differences between the Spongostan and the placebo film developed after subcutaneous implantation were observed. In contrast, the inflammation stage was reduced and the “scar” in the dermis was smaller when PVP-I and PVA/HPMC_B films were used. A smaller local inflammatory response inflicted less tissue damage, leading to the activation of subsequent regeneration phases and restoration of the area to its original state. The results obtained confirmed that PVP-I incorporated into PVA-hydroxypropyl methylcellulose film is a promising drug carrier, working faster and more effectively than the other two dressing materials evaluated. These developments provide a promising alternative in tissue regeneration and the wound healing process.
Research on the Pressure Dropin Horizontal Pneumatic Conveying for Large Coal Particles
Authors: Yang Daolong , Wang Yanxiang , Hu Zhengwei
Published: June 2020

As a type of airtight conveying mode, pneumatic conveying has the advantages of environmental friendliness and conveying without dust overflow. The application of the pneumatic conveying system in the field of coal particle conveying can avoid direct contact between coal particles and the atmosphere, which helps to reduce the concentration of air dust and improve environmental quality in coal production and coal consumption enterprises. In order to predict pressure drop in the pipe during the horizontal pneumatic conveying of large coal particles, the Lagrangian coupling method and DPM (discrete particle model) simulation model was used in this paper. Based on the comparison of the experimental results, the feasibility of the simulation was verified and the pressure drop in the pipe was simulated. The simulation results show that when the flow velocity is small, the simulation results of the DPM model are quite different from that of the experiment. When the flow velocity is large, the large particle horizontal pneumatic conveying behavior predicted by the model is feasible, which can provide a simulation reference for the design of the coal pneumatic conveying system.