bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on Rapid and Non-Destructive Detection of Coffee Powder Adulteration Based on Portable Near-Infrared Spectroscopy Technology
Authors: Zhang Fujie , Yu Xiaoning , Li Lixia , Song Wanxia , Dong Defeng , Yue Xiaoxian , Chen Shenao , Zeng Qingyu
Published: February 2025

This study explores the feasibility of using portable near-infrared spectroscopy for the rapid and non-destructive detection of coffee adulteration. Spectral data from adulterated coffee samples in the 900–1700 nm range were collected and processed using five preprocessing methods. For qualitative detection, the Support Vector Machine (SVM) algorithm was applied. For quantitative detection, two optimization algorithms, Invasive Weed Optimization (IWO) and Binary Chimp Optimization Algorithm (BChOA), were used for the feature wavelength selection. The results showed that convolution smoothing combined with multiple scattering correction effectively improved the signal-to-noise ratio. SVM achieved 96.88% accuracy for qualitative detection. For the quantitative analysis, the IWO algorithm identified key wavelengths, reducing data dimensionality by 82.46% and improving accuracy by 10.96%, reaching 92.25% accuracy. In conclusion, portable near-infrared spectroscopy technology can be used for the rapid and non-destructive qualitative and quantitative detection of coffee adulteration and can serve as a foundation for the further development of rapid, non-destructive testing devices. At the same time, this method has broad application potential and can be extended to various food products such as dairy, juice, grains, and meat for quality control, traceability, and adulteration detection. Through the feature wavelength selection method, it can effectively identify and extract spectral features associated with these food components (such as fat, protein, or characteristic compounds), thereby improving the accuracy and efficiency of detection, further ensuring food safety and enhancing the level of food quality control.
Self-Enhanced Near-Infrared Copper Nanoscale Electrochemiluminescence Probe for the Sensitive Detection of Ciprofloxacin in Foods
Authors: Wu Jie , Qin Yuanjie , Mei Xiaoxin , Cai Lin , Hao Wen , Fang Guozhen
Published: February 2025

Ciprofloxacin (CIP), a widely used broad-spectrum antibiotic, poses a serious threat to human health and environmental safety due to its residues. The complementary monomers molecularly imprinted electrochemiluminescence sensor (MIECLS) based on a polyvinylpyrrolidone-functionalized copper nanowires (CuNWs@PVP) luminescent probe was constructed for the ultra-sensitive detection of CIP. CuNWs with low cost and high conductivity exhibited near-infrared electrochemiluminescence (NIR ECL) properties, yet their self-aggregation and oxidation led to a weakened emission phenomenon. PVP with solvent affinity and large skeleton was in situ attached to CuNWs surface to avoid CuNWs sedimentation and aggregation, and self-enhanced ECL signals were achieved. The bifunctional monomers molecularly imprinted polymer (MIP) possessed complementary active centers that increased their affinity with CIP, enhancing the accurate and sensitive detection of the target substances. The linear range of CIP using MIECLS was 5.00 × 10−9–5.00 × 10−5 mol L−1 with a low limit of detection (LOD) of 2.59 × 10−9 mol L−1, while the recovery rates of CIP in the spiking recovery experiment were 84.39% to 92.48%. The combination of bifunctional monomer MIP and NIR copper-based nano-luminescent probe provides a new method for the detection of CIP in food.
A Short Note on Fractal Interpolation in the Space of Convex Lipschitz Functions
Authors: Gota Fatin , Massopust Peter
Published: February 2025

In this short note, we consider fractal interpolation in the Banach space Vθ(I) of convex Lipschitz functions defined on a compact interval I⊂R. To this end, we define an appropriate iterated function system and exhibit the associated Read–Bajraktarević operator T. We derive conditions for which T becomes a Ratkotch contraction on a closed subspace of Vθ(I), thus establishing the existence of fractal functions of class Vθ(I). An example illustrates the theoretical findings.
User Adoption of Electrified Powertrains: Identification of Factors Through Discrete Choice Modelling
Authors: Sica Lorenzo , Carboni Angela , Deflorio Francesco Paolo , Fappanni Filippo , Botta Cristiana
Published: February 2025

This study identified the main factors affecting car selection decisions through discrete choice experiments based on a large dataset collected in four European countries in 2023 using stated choice questionnaires. The choice set includes six current and popular car powertrains with factors related to vehicle features, user characteristics, and specific geographical contexts, which can influence the adoption of vehicles with electrified powertrains. An easily applicable multinomial logit model was first proposed to explore the effects of selected attributes and the model’s ability to reproduce user preferences with different incentive policies, geographical contexts, and energy prices. A mixed logit model and a segmented multinomial logit model were introduced to consider the sample’s heterogeneity. The first captures the preference dispersion among respondents related to incentives and operational costs. The second, which specifically classifies users based on car market segments, showed a greater variation in factors related to the purchase cost and battery range. The models estimate the weight of nine factors, offering support for targeted policy recommendations. Cost-related factors confirm their relevance in choices, and the analysis shows that users who want to enhance their vehicle range by 1 km are willing to pay approximately EUR 80.
Innovative Processing and Sterilization Techniques to Unlock the Potential of Silk Sericin for Biomedical Applications
Authors: Veiga Anabela , Ramírez-Jiménez Rosa Ana , Santos-Rosales Víctor , García-González Carlos A. , Aguilar Maria Rosa , Rojo Luis , Oliveira Ana L.
Published: February 2025

Silk sericin (SS), a by-product of the textile industry, has gained significant attention for its biomedical potential due to its biocompatibility and regenerative potential. However, the literature lacks information on SS processing methods and the resulting physicochemical properties. This study represents the first step in protocol optimization and standardization. In the present work, different processing techniques were studied and compared on SS extracted from boiling water: evaporation, rotary evaporation, lyophilization, and dialysis, which presented a recovery yield of approximately 27–32%. The goal was to find the most promising process to concentrate extracted SS solutions, and to ensure that the SS structure was highly preserved. As a result, a new cryo-lyophilization methodology was proposed. The proposed method allows for the preservation of the amorphous structure, which offers significant advantages including complete dissolution in water and PBS, an increase in storage stability, and the possibility of scaling-up, making it highly suitable for industrial and biomedical applications. The second part of the work focused on addressing another challenge in SS processing: efficient and non-destructive sterilization. Supercritical CO2 (scCO2) has been gaining momentum in the last years for sterilizing sensitive biopolymers and biological materials due to its non-toxicity and mild processing conditions. Thus, scCO2 technology was validated as a mild technique for the terminal sterilization of SS. In this way, it was possible to engineer a sequential cryo-lyophilization/scCO2 sterilization process which was able to preserve the original properties of this natural silk protein. Overall, we have valorized SS into a sterile, off-the-shelf, bioactive, and water-soluble material, with the potential to be used in the biomedical, pharmaceutical, or cosmetic industries.
Encapsulation of Lactoferrin in Calcium-Alginate Microparticles and Its Release Therefrom Under Neutral and Mild Acidic Conditions: Synthesis, Characterization and Mathematical Modeling
Authors: Paduano Teresa , Zuppolini Simona , Vitiello Rosa , Zarrelli Mauro , Tesser Riccardo , Borriello Anna
Published: February 2025

Bio-based polymeric stimuli-responsive materials have attracted increasing interest, especially in the pharmacological and nutraceutical fields. These materials mainly consist of macromolecules capable of conformational and chemical changes in response to external signals. One active molecule mostly used in bio-related areas is lactoferrin (Lf), which is attracting attention due to its beneficial effects (antimicrobial, anti-inflammatory, and anti-carcinogenic) on the human body. Since pH or temperature in the human body can promote Lf degradation, encapsulation in a suitable system is required. A valid solution is to encapsulate the Lf in a polysaccharidic matrix such as alginate (ALG) thanks to its biocompatibility and easy gelation with bivalent cations. This work aims to encapsulate iron-depleted Lf in alginate gel microspheres for stability improvement by ionic cross-linking with Ca2+ ions. The obtained particles were characterized in terms of structure, thermal stability, and morphology, and their swelling capability was determined. Release studies were carried out on the freeze-dried particles to investigate the effect of neutral pH 7 and acidic pH 5. At last, the optimization of the loaded system was completed by developing a mathematical model able to predict the swelling behavior of the carrier particle and the subsequent Lf kinetic release over time.
Amine-Functionalized Gellan Gum-Based Hydrogel Loaded with Adipose Stem Cell-Derived Small Extracellular Vesicles: An In Vitro Proof of Concept for Enhancing Diabetic Foot Ulcer Healing
Authors: Tomasello Laura , Biondo Mattia , Biscari Giuseppina , Di Rosa Luigi , Palumbo Fabio Salvatore , Fiorica Calogero , Pitarresi Giovanna , Vasto Sonya , Pizzolanti Giuseppe , Arnaldi Giorgio
Published: February 2025

Diabetic foot ulcers (DFUs) are chronic wounds and a common complication of diabetes. A promising strategy in the treatment of DFUs involves the use of stem cell derivatives, such as small extracellular vesicles (sEVs), which can enhance cell proliferation and reduce inflammation while avoiding immunogenic responses. In this study, we evaluated the ability of adipose mesenchymal stem cell- (ASC)-derived sEVs to enhance the proliferation of human fibroblasts, which play a crucial role in wound regenerative processes. To mimic the inflammatory environment of DFUs, fibroblasts were cultured into the gellan gum (GG) modified with ethylenediamine (EDA) hydrogel scaffolds loaded with ASC-derived sEVs, under pro-inflammatory cytokines. Our comparative analysis demonstrated that sEVs loaded in GG-EDA hydrogel improved fibroblast viability in pro-inflamed conditions while retaining the anti-inflammatory and immunomodulatory properties of their cells of origin. By modulating the gene expression profile of fibroblasts to promote cell proliferation, wound healing and re-epithelialization, our system presents a promising therapeutic strategy for DFU healing.
Advances in Remote Sensing and Deep Learning in Coastal Boundary Extraction for Erosion Monitoring
Authors: Blais Marc-André , Akhloufi Moulay A.
Published: February 2025

Erosion is a critical geological process that degrades soil and poses significant risks to human settlements and natural habitats. As climate change intensifies, effective coastal erosion management and prevention have become essential for our society and the health of our planet. Given the vast extent of coastal areas, erosion management efforts must prioritize the most vulnerable and critical regions. Identifying and prioritizing these areas is a complex task that requires the accurate monitoring and forecasting of erosion and its potential impacts. Various tools and techniques have been proposed to assess the risks, impacts and rates of coastal erosion. Specialized methods, such as the Coastal Vulnerability Index, have been specifically designed to evaluate the susceptibility of coastal areas to erosion. Coastal boundaries, a critical factor in coastal erosion monitoring, are typically extracted from remote sensing images. Due to the extensive scale of coastal areas and the complexity of the data, manually extracting coastal boundaries is challenging. Recently, artificial intelligence, particularly deep learning, has emerged as a promising and essential tool for this task. This review provides an in-depth analysis of remote sensing and deep learning for extracting coastal boundaries to assist in erosion monitoring. Various remote sensing imaging modalities (optical, thermal, radar), platforms (satellites, drones) and datasets are first presented to provide the context for this field. Artificial intelligence and its associated metrics are then discussed, followed by an exploration of deep learning algorithms for extracting coastal boundaries. The presented algorithms range from basic convolutional networks to encoder–decoder architectures and attention mechanisms. An overview of how these extracted boundaries and other deep learning algorithms can be utilized for monitoring coastal erosion is also provided. Finally, the current gaps, limitations and potential future directions in this field are identified. This review aims to offer critical insights into the future of erosion monitoring and management through deep learning-based boundary extraction.
Numerical Analysis of Jacked and Impact-Driven Pile Installation Procedures in Offshore Wind Turbine Foundations
Authors: Chan Ka Lok , Lopez-Querol Susana , Martin-Moreta Pedro
Published: February 2025

The increasing global demand for renewable energy has resulted in a high interest in wind power, with offshore wind farms offering better performance than onshore installations. Coastal nations are thus, actively developing offshore wind turbines, where monopiles are the predominant foundation type. Despite their widespread use, the effects of monopile installation methods on the overall foundation behaviour are not sufficiently yet understood. This study investigates how different pile installation procedures—jacked and impact-driven—affect the lateral capacity of monopile foundations under both monotonic and dynamic lateral loads, by comparing them with wished-in-place monopiles, the usual assumption in design, for which no soil disturbance due to installation is considered. Three finite element 3D models were employed to simulate these cases, i.e., wished-in-place monopile, jacked, and impact-driven pile, incorporating soil zoning in the latter cases to replicate the effects of the installation methods. Comparisons between all these models, when subject to lateral monotonic and cyclic loads, are presented and discussed in terms of displacements in the soil and horizontal normal stresses. Results reveal that these installation methods significantly influence soil reactions, impacting the lateral performance of monopiles under both monotonic and dynamic conditions. The impact-driven pile demonstrated the most significant influence on the monopile behaviour. These findings highlight the need for engineers to account for installation effects in the design of monopile foundations to enhance performance and reliability, as well as the optimisation of their design.
Impact of a 10-Week Aqua Fitness Intervention on Physical Fitness and Psychosocial Measures in Inactive Healthy Adult Women
Authors: Dalamitros Athanasios A. , Kouloglou Aristotelis , Nasoufidis Giorgos , Stogiannidou Kleopatra , Eradli Nur , Manou Vasiliki
Published: February 2025

Background/objectives: Previous studies on aquatic exercises have primarily focused on either physical fitness or psychological outcomes. This study examines the effects of a structured 10-week aqua fitness program on physical fitness and psychosocial outcomes in healthy adult women. Additionally, a 4-week training cessation period was incorporated to assess the sustainability of any observed physical fitness benefits. Methods: A total of 32 female participants (mean age 51.28 ± 9.12 years) with prior aqua aerobics experience engaged in supervised aqua fitness sessions, conducted three times per week (~55 min/session) at moderate intensity (RPE = 12, on a 6–20 scale). The physical fitness outcomes assessed included dominant hand grip strength, lower limb muscle endurance, dynamic balance, mobility, and upper and lower limb flexibility. The psychosocial outcomes included subjective well-being and social inclusion. Results: The results demonstrate significant improvements in dynamic balance (ES = 0.85) and lower limb flexibility (ES = 0.73 and 0.65 for the two limbs, respectively), with smaller yet notable gains observed in other physical fitness outcomes (ES = from 0.20 to 0.48). On the contrary, only a marginal improvement was detected in a single domain of subjective well-being (environmental domain, ES = 0.35) and no changes were observed across the seven domains of social inclusion. Importantly, all physical fitness gains were maintained during the 4-week training cessation period, with lower limb flexibility showing additional improvements. Conclusions: These findings underscore the effectiveness of supervised aqua fitness programs in enhancing physical fitness in middle-aged women, while their impact on psychosocial outcomes appears limited in this population.
Influence of Gender Role on Resilience and Positive Affect in Female Nursing Students: A Cross-Sectional Study
Authors: Mayor-Silva L. Iván , Moreno Guillermo , Meneses-Monroy Alfonso , Martín-Casas Patricia , Hernández-Martín Marta M. , Moreno-Pimentel Antonio G. , Rodríguez-Leal Leyre
Published: February 2025

Introduction: Women experience more social barriers, gender stereotypes, biases, and discrimination than men, which can increase their vulnerability to mental health problems. Therefore, it is essential to adopt a gender perspective in research on nursing students, examining the impact of these factors on their well-being and psychological resources like resilience. This study aims to analyze the relationship between gender roles in resilience and positive or negative affect among female nursing students. Methods: A cross-sectional study was conducted with first- and fourth-year female nursing students at a public university in Madrid, Spain. Sociodemographic variables, positive and negative affect (PANAS scale), resilience (CD-RISC scale), and gender roles (BRSI inventory) were analyzed. ANOVA, correlation analysis, and linear regression models were used to study the relationships between variables. Results: The study included 338 students with a mean age of 21.43 years, of which 80.2% had a high level of resilience, with a positive affect score of 31.96 (SD: 7.34) and a negative affect score of 22.99 (SD: 7.35). Overall, 48.5% had undifferentiated roles, 23.7% feminine roles, 14.2% androgynous roles, and 13.6% masculine roles. Female students with masculine and androgynous roles showed higher resilience levels (93.48% and 97.92%) compared to those with feminine and undifferentiated roles (81.25% and 70.73%) (p < 0.001). Female students with androgynous and masculine roles showed higher positive affect levels compared to those with feminine and undifferentiated roles (p < 0.001), with no differences in negative affect. These results were observed in both first- and fourth-year students. A high correlation was found between masculine roles and positive affect and resilience in both first- and fourth-year students. Conclusions: Gender roles influence positive affect and resilience in females. Among female nursing students, androgynous and masculine roles are associated with higher levels of resilience and positive affect compared to feminine and undifferentiated roles. Differences in psychological well-being may be related to socially constructed gender roles rather than biological sex, with masculine roles enhancing resilience and feminine roles correlating with greater vulnerability.
Severity Benchmarks for the Level of Personality Functioning Scale—Brief Form 2.0 (LPFS-BF 2.0) in Polish Adults
Authors: Juras Karolina , Mendrok Mateusz , Pach Janusz , Moroń Marcin
Published: February 2025

Background/Objectives: The Level of Personality Functioning Scale—Brief Form 2.0 (LPFS-BF 2.0) is a self-report screening measure of personality impairments according to the DSM-5 Alternative Model for Personality Disorders and the ICD-11 classification of personality disorders. Nevertheless, reliable cut-off scores that could help in clinical decision making are still lacking for many populations. The aim of this study was to develop severity benchmarks of the LPFS-BF 2.0 for a Polish population based on the item response theory (IRT) approach. Methods: A sample of Polish adults (n = 530) took part in the study. The participants assessed their personality functioning and pathological personality traits and provided information about psychiatric diagnosis and psychotherapy seeking. The severity benchmarks were developed using IRT and validated using mean and frequency comparisons between groups of different personality impairments according to the developed cut-offs. Results: Confirmatory factor analysis (CFA) supported a unidimensional model of the LPFS-BF 2.0. The graded IRT model indicated satisfactory item functioning for all LPFS-BF 2.0 items. The normative observed score thresholds at different latent severity levels of personality impairments were developed, and significant overall differences were found between the LPFS-BF 2.0 norm-based severity benchmarks in pathological personality traits and psychotherapy seeking. Conclusions: The IRT-based cut-offs for the LPFS-BF 2.0 identified individuals high on pathological personality traits (particularly disinhibition) and were predictive of psychotherapy seeking. The developed severity benchmarks allow for the interpretation of LPFS-BF 2.0 scores, supporting clinical diagnosis and relevant decision making in the Polish population. Practical implications for healthcare practice and research are being discussed.
Anxiety and Depression and Related Risk Factors in Italian Healthcare Providers Involved in Adverse Events
Authors: Busch Isolde Martina , Mazzi Maria Angela , Cosci Fiammetta , Berti Loretta , Marinelli Veronica , Moretti Francesca , Maggioni Olga , Wu Albert W. , Rimondini Michela
Published: February 2025

Background/Objectives: Despite the importance of the second victim phenomenon for healthcare systems, there is limited research on Italian healthcare providers. We assessed emotional distress in individuals impacted by an adverse event using the Withstand-PSY Questionnaire (WS-PSY-Q). Additionally, we aimed to identify potential risk factors for anxiety and depression. Methods: A cross-sectional online survey of 284 participants. Measures included the WS-PSY-Q, Beck Depression Inventory-II (BDI-II), and State-Trait Anxiety Inventory (STAI-Y). Descriptive analyses and seemingly unrelated regression, jointly estimating anxiety and depression, were conducted using Stata (version 18). Results: Fifty-nine percent of the participants tested positive for anxiety (WS-PSY-Q anxiety subscale ≥ 16), thirty-seven percent for depression (WS-PSY-Q depression subscale ≥ 22), and thirty-five percent for both. In the final model, anxiety symptoms following the adverse event were associated with pre-event anxiety levels (p < 0.01), seeking psychological help (p < 0.05), self-perceived responsibility (p < 0.01), severity of the adverse event for the patient (p < 0.05), and punitive workplace climate (p < 0.05). Correlates of post-event depressive symptoms included pre-existing depression (p < 0.01), self-perceived responsibility (p < 0.01), severity of the impact of the adverse event (p < 0.01), punitive or neutral workplace climate (p < 0.05), and seeking psychological help (p < 0.01). Conclusions: This study adds to the growing understanding of the mental health difficulties that healthcare workers in Italy encounter after adverse events, addressing both individual and systemic risk factors. Proactive implementation of mental health measures for healthcare workers could foster their well-being after adverse events and promote a stronger, more just organizational culture.
A Cross-Sectional Survey on the Management of Medication Adherence Among Healthcare Professionals in Saudi Arabia
Authors: Khawagi Wael Y. , Baali Fahad H. , Alnefaie Norah M. , Albishi Shatha A. , Al-swat Alla H. , Alshahrani Dinan A. , Alshemaimri Ragad A. , Alshehri Abdullah A.
Published: February 2025

Background/Objectives: Medication adherence is essential for effective healthcare, significantly influencing treatment success and overall health outcomes. However, there is limited understanding of how healthcare professionals in Saudi Arabia manage and support medication adherence. This study aims to examine physicians’ and pharmacists’ approaches to managing medication adherence in Saudi Arabia by examining the methods used for adherence assessment, interventions to enhance adherence, and the challenges faced. Methods: A cross-sectional study was conducted over nine months using a self-administered online questionnaire. The study targeted physicians and pharmacists actively working in Saudi Arabia. The questionnaire was distributed through professional networks to ensure a broad and representative sample. Results: A total of 397 healthcare professionals met the inclusion criteria, comprising 81.1% pharmacists and 18.9% physicians. Direct patient inquiry was the most common assessment method, frequently or always used by 81.3% of physicians and 57.1% of pharmacists. Treatment response evaluation was similarly frequent (89.3% of physicians and 56.2% of pharmacists). Standardized tools, such as the Morisky Medication Adherence Scale, were underutilized (14.7%). Adherence-enhancing interventions focused on patient education, and their use was reported by 89.3% of physicians and 74.2% of pharmacists as frequent or always. Written information was more commonly used by pharmacists (65.8%) than physicians (45.3%). Barriers included excessive workloads and short consultation times (59.9%), absence of effective systems for tracking adherence (51.9%), lack of reliable tools for assessing adherence (48.9%), and insufficient training in behavioral interventions (48.1%). Conclusions: This study reveals significant differences in medication adherence management practices between physicians and pharmacists in Saudi Arabia, emphasizing their distinct roles. Key barriers, including excessive workload, limited consultation time, and inadequate tracking systems, hinder the adoption of evidence-based tools. Tailored interventions, enhanced interprofessional collaboration, and systemic support are essential to address these challenges and improve adherence management, ultimately enhancing patient outcomes.
Perceptions of Nurse–Surgeon Communication in the Operating Room: A Q-Methodology Study
Authors: An Jeong Hwa , Yeun Eun Ja , Lee Sul Hee , Kim Ho Young
Published: February 2025

Inadequate communication between nurses and surgeons can lead to patient injuries and increased healthcare costs. This study aimed to identify and understand subjective perceptions of communication between Korean nurses and surgeons in operating rooms (ORs), focusing on their values, beliefs, and attitudes. The Q-methodology was employed in this research, as it integrates the strengths of both quantitative and qualitative approaches. A convenience sample of 46 participants classified 45 Q-statements by using a nine-point forced normal grid. The collected data were analyzed by using by-person factor analysis with the pc-QUANL program. This study revealed four distinct types of nurse–surgeon communication in the OR: professional communication, cynical conflict, passive task-oriented communication, and relationship-oriented endurance. These four types accounted for 58.7% of the total variance, distributed as follows: type 1 (38.7%), type 2 (9.8%), type 3 (5.4%), and type 4 (4.8%). The eigenvalues were 15.8, 4.1, 2.2, and 1.9, respectively. Based on these findings, tailored strategies to enhance nurse–surgeon communication according to each type are essential. Enhancing communication dynamics can lead to more effective interactions, improve patient care and safety, and boost job satisfaction among healthcare professionals. The results have significant implications for healthcare organizations and nursing managers aiming to improve nurses’ communication skills. Additionally, this study provides insights for healthcare organizations in other countries regarding the communication competence traits among Korean health professionals.
The Interplay of Politics and Conspiracy Theories in Shaping Vaccine Hesitancy in a Diverse Cultural Setting in Italy
Authors: Wiedermann Christian J. , Plagg Barbara , Rina Patrick , Piccoliori Giuliano , Engl Adolf
Published: February 2025

Vaccine hesitancy presents a significant challenge to public health, particularly in culturally diverse regions, such as South Tyrol, Italy. This article examines the interplay between political influences, conspiracy theories, and vaccine hesitancy in South Tyrol, an autonomous province characterised by its linguistic diversity and historical scepticism toward central authority. This study aimed to identify the important drivers of vaccine hesitancy and propose targeted strategies to enhance vaccine acceptance. Peer-reviewed and grey literature was examined to explore the sociocultural factors, political dynamics, and conspiracy narratives influencing vaccine hesitancy in South Tyrol. The analysis incorporated publicly available materials, including propaganda from anti-vaccine organisations, and regional public health data to contextualise the findings. Vaccine hesitancy in South Tyrol was influenced by historical tensions with the central government, cultural alignment with Austrian healthcare practices, and politically motivated opposition to vaccination. Conspiracy theories disseminated by local organisations and political entities exploit concerns regarding governmental overreach and personal autonomy. These dynamics are compounded by the selective misrepresentation of scientific discourse, which further polarises public opinion. Addressing vaccine hesitancy in South Tyrol requires culturally sensitive communication, community engagement through trusted local figures, transparency in health policies, and the proactive monitoring of misinformation. These strategies can mitigate mistrust and promote vaccine acceptance in regions with similar sociopolitical complexities.
Effects of Climate Change on Health and Health Systems: A Systematic Review of Preparedness, Resilience, and Challenges
Authors: Gkouliaveras Vasileios , Kalogiannidis Stavros , Kalfas Dimitrios , Kontsas Stamatis
Published: February 2025

Climate change has a significant impact on the population’s health and negatively affects the functioning of healthcare systems. Health systems must be operationally prepared to handle the challenges posed by environmental change. Resilience is required to adapt quickly to critical environmental conditions and reduce carbon emissions. In this systematic review strategies, for health system preparedness and resilience are examined to address the impacts of climate change, and the barriers and challenges faced when implementing them. To identify studies, the Scopus, PubMed and Google Scholar databases were searched three times (from April to October 2024, 21 April, 15 June, and 9 September) for the years 2018 to 2024, using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) methodology. Specifically, the search identified 471 articles, of which the specified inclusion and exclusion criteria (secondary studies with inclusion criteria, being in English, etc.) were met by sixteen (16) studies. According to the findings of the studies reviewed, adaptation strategies focus on structural changes, the development of training programs, the development of surveillance systems, and appropriate operational plans. The leader’s ability to motivate employees to achieve defined goals, continuous evaluation of goals and interventions, and learning from previous disasters play an important role in their implementation. Similarly, key policies and strategies for mitigation include the adoption of sustainable practices, such as recycling and cultural change. However, lack of resources (human, material, financial) and increased demand for health services make it difficult to implement adaptation and mitigation strategies. The findings of the review are mainly theoretical in nature and are confirmed by other studies. It is suggested that further research on resilience and preparedness of health systems should be pursued, leading to their sustainability and the formulation of appropriate policies.
Tunisian Pediatricians’ Attitudes and Practices Toward COVID-19 Immunization and Other Vaccines
Authors: Cherif Ines , Gharbi Rabeb , Kharroubi Ghassen , Affes Walid , Bettaieb Jihene
Published: February 2025

Pediatricians are among the most trusted sources of vaccine information for parents. We aimed, in this study, to describe the attitudes and practices of Tunisian pediatricians regarding non-National Immunization Schedule (NIS) vaccines, specifically the COVID-19 vaccination for children, and to identify factors associated with their willingness to recommend it. We conducted a national cross-sectional study among Tunisian pediatricians between July and October 2023 using a standardized questionnaire administered face-to-face. We calculated prevalence with 95% confidence intervals (95%CIs) and adjusted odds ratios (aOR) using multivariable logistic regression. Of 330 contacted pediatricians, 192 (58.2%) responded (mean age: 50.9 ± 12.9 years). The majority (89.1%, 95% CI: [84.6–93.5]) said that they recommend other vaccines that are not part of the NIS and 40.6% [33.7–47.6] declared their willingness to recommend the COVID-19 vaccination for children. The odds of pediatricians willing to recommend the COVID-19 vaccination for children were higher among those who believed that this vaccine would reduce school absenteeism (aOR = 2.3 [1.1–5.1]) and among those who have great confidence in the Ministry of Health’s recommendations regarding COVID-19 vaccination (aOR = 6.1 [2.2–16.9]). More than half of the pediatricians in Tunisia recommend other vaccines that are not part of the NIS but show hesitancy toward the COVID-19 vaccine. Thus, involving pediatricians in the decision-making process for childhood vaccination strategies is crucial.
Biological Activities of Constituents from Rosa roxburghii and Their Mechanisms Based on Network Pharmacology and Biological Verification
Authors: Xiang Li-Juan , Zhang Shuang , Luo Ming-Liang , Long Xing-Xiang , Zhou Ying , Yin Xin
Published: February 2025

Rosa roxburghii Tratt is widely cultivated in southwestern areas of China for a range of purposes, including food and medicine. To enhance its application value, one previously undescribed compound (1) and six known compounds (2–7) were isolated from the roots of R. roxburghii. The structures of compounds (1–7) were determined through NMR, HR-MS, and CD experiments, and by comparison of their spectroscopic data with values from the literature. Roxbubenzoate A (1) contained a benzoyl glucuronosyl glycerol scaffold featuring a rare α-glucuronosyl linkage. Seven compounds (1–7) were tested for their anti-inflammatory, α-glucosidase-inhibitory, and radical-scavenging activities. Compound 3 showed a significant inhibitory effect on the release of NO in LPS-induced RAW264.7 macrophages, with an IC50 value of 7.8 ± 0.2 μM. Compounds 2, 4, and 7 exhibited moderate inhibitory activity against α-glucosidase, with inhibition rates of 50.1%, 46.7%, and 41.1% at a concentration of 200 μM, respectively. Compounds 1, 2, and 6 exhibited moderate ABTS radical-scavenging activity, with IC50 values of 107.0 ± 1.1, 142.6 ± 0.8, and 128.3 ± 1.2 μM, respectively. The network pharmacological analysis and molecular docking results suggested that 3 may be able to treat inflammation by binding TNF-α and IL-6 targets. Finally, the expression of IL-6 and TNF-α in LPS-induced RAW264.7 macrophages was detected through ELISA, and 3 showed a strong inhibitory effect on IL-6 release. These findings offer a novel perspective on the development of rich medicinal plant resources from R. roxburghii roots.
Role of 11β-Hydroxysteroid Dehydrogenase and Mineralocorticoid Receptor on Alzheimer’s Disease Onset: A Systematic Review
Authors: Di Vincenzo Mariangela , Pellegrino Pamela , Schiappa Genny , Campanati Anna , Del Vescovo Valerio , Piccirillo Silvia , Ambrogini Patrizia , Arnaldi Giorgio , Orciani Monia
Published: February 2025

The role of 11β-HSD1 in Alzheimer’s disease (AD) has garnered significant attention due to its involvement in glucocorticoid metabolism, neuroinflammation, and cognitive decline. This review explores the current understanding of 11β-HSD1 in AD, examining genetic, preclinical, and clinical research. Genetic studies have identified 11β-HSD1 polymorphisms that may influence AD risk, although findings remain inconsistent. Mechanistically, 11β-HSD1 promotes neurodegeneration through the dysregulation of glucocorticoid activity, contributing to hippocampal atrophy, amyloid plaque formation, and tau pathology. Preclinical studies have shown that 11β-HSD1 inhibitors offer neuroprotective effects, including enhanced cognitive function, reduced inflammation, and improved mitochondrial activity. However, clinical trials, including those involving ABT-384 and Xanamem, have produced mixed results, with no substantial cognitive improvements despite effective enzyme inhibition. These inconsistencies highlight the complexity of AD and the challenges in translating preclinical findings into clinical outcomes. Moreover, while 11β-HSD1 inhibition holds therapeutic potential, other strategies targeting neuroinflammation, autophagy, and glucocorticoid signaling are also being explored. Ongoing research is focusing on optimizing 11β-HSD1 inhibitors, identifying biomarkers for patient selection, and investigating combination therapies to enhance treatment efficacy. Ultimately, 11β-HSD1’s role in AD presents a promising therapeutic target, but further studies are required to fully understand its potential in managing the disease.
Impact of African-Specific ACE2 Polymorphisms on Omicron BA.4/5 RBD Binding and Allosteric Communication Within the ACE2–RBD Protein Complex
Authors: Barozi Victor , Tastan Bishop Özlem
Published: February 2025

Severe acute respiratory symptom coronavirus 2 (SARS-CoV-2) infection occurs via the attachment of the spike (S) protein’s receptor binding domain (RBD) to human ACE2 (hACE2). Natural polymorphisms in hACE2, particularly at the interface, may alter RBD–hACE2 interactions, potentially affecting viral infectivity across populations. This study identified the effects of six naturally occurring hACE2 polymorphisms with high allele frequency in the African population (S19P, K26R, M82I, K341R, N546D and D597Q) on the interaction with the S protein RBD of the BA.4/5 Omicron sub-lineage through post-molecular dynamics (MD), inter-protein interaction and dynamic residue network (DRN) analyses. Inter-protein interaction analysis suggested that the K26R variation, with the highest interactions, aligns with reports of enhanced RBD binding and increased SARS-CoV-2 susceptibility. Conversely, S19P, showing the fewest interactions and largest inter-protein distances, agrees with studies indicating it hinders RBD binding. The hACE2 M82I substitution destabilized RBD–hACE2 interactions, reducing contact frequency from 92 (WT) to 27. The K341R hACE2 variant, located distally, had allosteric effects that increased RBD–hACE2 contacts compared to WThACE2. This polymorphism has been linked to enhanced affinity for Alpha, Beta and Delta lineages. DRN analyses revealed that hACE2 polymorphisms may alter the interaction networks, especially in key residues involved in enzyme activity and RBD binding. Notably, S19P may weaken hACE2–RBD interactions, while M82I showed reduced centrality of zinc and chloride-coordinating residues, hinting at impaired communication pathways. Overall, our findings show that hACE2 polymorphisms affect S BA.4/5 RBD stability and modulate spike RBD–hACE2 interactions, potentially influencing SARS-CoV-2 infectivity—key insights for vaccine and therapeutic development.
Correlations Between Gut Microbiota Composition, Medical Nutrition Therapy, and Insulin Resistance in Pregnancy—A Narrative Review
Authors: Enache Robert-Mihai , Roşu Oana Alexandra , Profir Monica , Pavelescu Luciana Alexandra , Creţoiu Sanda Maria , Gaspar Bogdan Severus
Published: February 2025

Many physiological changes accompany pregnancy, most of them involving metabolic perturbations. Alterations in microbiota composition occur both before and during pregnancy and have recently been correlated with an important role in the development of metabolic complications, such as insulin resistance and gestational diabetes mellitus (GDM). These changes may be influenced by physiological adaptations to pregnancy itself, as well as by dietary modifications during gestation. Medical nutritional therapy (MNT) applied to pregnant women at risk stands out as one of the most important factors in increasing the microbiota’s diversity at both the species and genus levels. In this review, we discuss the physiological changes during pregnancy and their impact on the composition of the intestinal microbiota, which may contribute to GDM. We also discuss findings from previous studies regarding the effectiveness of MNT in reducing insulin resistance. In the future, additional studies should aim to identify specific gut microbial profiles that serve as early indicators of insulin resistance during gestation. Early diagnosis, achievable through stool analysis or metabolite profiling, may facilitate the timely implementation of dietary or pharmaceutical modifications, thereby mitigating the development of insulin resistance and its associated sequelae.
Antibiofilm Activities of Halogenated Pyrimidines Against Enterohemorrhagic Escherichia coli O157:H7
Authors: Jeon Hyejin , Kim Yong-Guy , Lee Jin-Hyung , Lee Jintae
Published: February 2025

Enterohemorrhagic Escherichia coli (EHEC) is a significant public health concern due to its ability to form biofilms, enhancing its resistance to antimicrobials and contributing to its persistence in food processing environments. Traditional antibiotics often fail to target these biofilms effectively, leading to increased bacterial resistance. This study aims to explore the efficacy of novel antibiofilm agents, specifically halogenated pyrimidine derivatives, against EHEC. We screened pyrimidine and 31 halogenated pyrimidine derivatives for their antimicrobial and antibiofilm activities against EHEC using biofilm quantification assays, SEM analysis, motility, and curli production assessments. Our findings reveal that certain halogenated pyrimidine derivatives, notably 2-amino-5-bromopyrimidine (2A5BP), 2-amino-4-chloropyrrolo[2,3-d]pyrimidine (2A4CPP), and 2,4-dichloro-5-iodo-7H-pyrrolo[2,3-d]pyrimidine (2,4DC5IPP) at 50 µg/mL, exhibited significant inhibitory effects on EHEC biofilm formation without affecting bacterial growth, suggesting a targeted antibiofilm action. These compounds effectively reduced curli production and EHEC motility, essential factors for biofilm integrity and development. qRT-PCR analysis revealed that two active compounds downregulated the expression of key curli genes (csgA and csgB), leading to reduced bacterial adhesion and biofilm formation. Additionally, in silico ADME–Tox profiles indicated that these compounds exhibit favorable drug-like properties and lower toxicity compared with traditional pyrimidine. This study highlights the potential of halogenated pyrimidine derivatives as effective antibiofilm agents against EHEC, offering a promising strategy for enhancing food safety and controlling EHEC infections. The distinct mechanisms of action of these compounds, particularly in inhibiting biofilm formation and virulence factors without promoting bacterial resistance, underscore their therapeutic potential.
Bisabolane Sesquiterpenes with Anti-Chlamydial Activity Isolated from Ligularia narynensis
Authors: Gao Na , He Yi-Lin , Qi Hui-Ming , Yang Hong-Ying , Li Guo-Li , Li Zhao-Cai , Shen Tong
Published: February 2025

Chlamydia are obligate intracellular bacterial pathogens affecting humans and animals, causing miscarriage, stillbirth, or weak fetuses in the late stages of pregnancy of goats and sheep. Because there is no commercial vaccine for chlamydia in animals, drug treatment has become the most effective curative method. Natural products, also known as secondary metabolites, are becoming one of the main sources used in new drug development because of their structural diversity and biodiversity. In natural products, plant sources play a major role in the development process of new drugs. In this study, five undescribed highly oxygenated bisabolane sesquiterpenes (Pararubin W, Pararubin X, Pararubin Y., Pararubin Z, and Pararubin AA) were isolated from whole plants of Ligularia narynensis. Their chemical structures were determined via analyses of HRESIMS, IR, 1D, and 2D NMR data, along with the assignment of their relative configurations. These compounds were tested for their anti-chlamydial activity. The results show that compounds 1 and 5 inhibited the growth of Chlamydia abortus in host cells in a dose-dependent manner.
SMURF1-Induced Ubiquitination of FTH1 Disrupts Iron Homeostasis and Suppresses Myogenesis
Authors: Xiong Xia , Li Wen , Yu Chunlin , Qiu Mohan , Zhang Zengrong , Hu Chenming , Zhu Shiliang , Yang Li , Pen Han , Song Xiaoyan , Chen Jialei , Xia Bo , Han Shunshun , Yang Chaowu
Published: February 2025

Ferritin heavy chain 1 (FTH1) is pivotal in the storage, release, and utilization of iron, plays a crucial role in the ferroptosis pathway, and exerts significant impacts on various diseases. Iron influences skeletal muscle development and health by promoting cell growth, ensuring energy metabolism and ATP synthesis, maintaining oxygen supply, and facilitating protein synthesis. However, the precise molecular mechanisms underlying iron’s regulation of skeletal muscle growth and development remain elusive. In this study, we demonstrated that the conditional knockout (cKO) of FTH1 in skeletal muscle results in muscle atrophy and impaired exercise endurance. In vitro studies using FTH1 cKO myoblasts revealed notable decreases in GSH concentrations, elevated levels of lipid peroxidation, and the substantial accumulation of Fe2+, collectively implying the induction of ferroptosis. Mechanistically, E3 ubiquitin-protein ligase SMURF1 (SMURF1) acts as an E3 ubiquitin ligase for FTH1, thereby facilitating the ubiquitination and subsequent degradation of FTH1. Consequently, this activation of the ferroptosis pathway by SMURF1 impedes myoblast differentiation into myotubes. This study identifies FTH1 as a novel regulator of muscle cell differentiation and skeletal muscle development, implicating its potential significance in maintaining skeletal muscle health through the regulation of iron homeostasis.