bluuebunny/crossref_metadata_2025 huggingface dataset:
High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in Populus
Authors: Li Zhiqun , Zhao Yifan , Cheng Xuetong , Kong Bo , Sang Yaru , Zhou Qing , Wu Jian , Zhang Pingdong
Published: April 2023

High temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in Populus canescens. However, the mechanism by which high temperature induces pollen abortion remains unknown. Here, pollen abortion was induced by exposing male flower buds of P. canescens to 38 and 41 °C; pollen morphology, meiotic abnormalities, defects of the meiotic microtubular cytoskeleton, and tapetum development were characterized. We found that the dominant meiotic stage, temperature, and duration of treatment significantly affected the percentage of high temperature-induced aborted pollen. Damaged spindle microtubules and depolymerized microtubular cytoskeletons were observed, which resulted in many lagging chromosomes at anaphase I and II as well as aneuploid male gametes and micronuclei, thereby generating aborted pollen grains. Tapetum disintegration was also delayed. The anther dehisced normally, and some viable pollen grains were released. However, no sufficient evidence showed that the delayed degradation of the tapetum was responsible for pollen abortion.
Computational Analysis Reveals Distinctive Interaction of miRNAs with Target Genes in the Pathogenesis of Chronic Kidney Disease
Authors: Salim Hafiz , Dandare Abdullahi , Khalil Fareeha , Liaquat Afrose , Khan Muhammad , Naeem Aisha
Published: April 2023

The regulation of genes is crucial for maintaining a healthy intracellular environment, and any dysregulation of gene expression leads to several pathological complications. It is known that many diseases, including kidney diseases, are regulated by miRNAs. However, the data on the use of miRNAs as biomarkers for the diagnosis and treatment of chronic kidney disease (CKD) are not conclusive. The purpose of this study was to elucidate the potential of miRNAs as an efficient biomarker for the detection and treatment of CKD at its early stages. Gene expression profiling data were acquired from the Gene Expression Omnibus (GEO) and differentially expressed genes (DEGs) were identified. miRNAs directly associated with CKD were obtained from an extensive literature search. Network illustration of miRNAs and their projected target differentially expressed genes (tDEGs) was accomplished, followed by functional enrichment analysis. hsa-miR-1-3p, hsa-miR-206, hsa-miR-494 and hsa-miR-577 exhibited a strong association with CKD through the regulation of genes involved in signal transduction, cell proliferation, the regulation of transcription and apoptotic process. All these miRNAs have shown significant contributions to the inflammatory response and the processes which eventually lead to the pathogenesis of CKD. The in silico approach used in this research represents a comprehensive analysis of identified miRNAs and their target genes for the identification of molecular markers of disease processes. The outcomes of the study recommend further efforts for developing miRNA biomarkers set for the early diagnosis of CKD.
Outlining the Clinical Profile of TCIRG1 14 Variants including 5 Novels with Overview of ARO Phenotype and Ethnic Impact in 20 Egyptian Families
Authors: El-Kamah Ghada Y. , Mehrez Mennat I. , Taher Mohamed B. , El-Bassyouni Hala T. , Gaber Khaled R. , Amr Khalda S.
Published: April 2023

TCIRG1 gene mutations underlie osteopetrosis, a rare genetic disorder impacting osteoclast function with consequent brittle bones prone to fracture, in spite of being characterized by increased bone density. The disorder is known to exhibit marked genetic heterogeneity, has no treatment, and is lethal in most instances. There are reports of ethnic variations affecting bone mineral density and variants’ expression as diverse phenotypes even within individuals descending from the same pedigree. We herein focus on one of osteopetrosis’s three types: the autosomal recessive malignant form (MIM 259700) (ARO) that is almost always associated with severe clinical symptoms. We reviewed the results of about 1800 Egyptian exomes and we did not detect similar variants within our Egyptian dataset and secondary neurological deficit. We studied twenty Egyptian families: sixteen ARO patients, ten carrier parents with at least one ARO affected sib, and two fetuses. They were all subjected to thorough evaluation and TCIRG1 gene sequencing. Our results of twenty-eight individuals descending from twenty Egyptian pedigrees with at least one ARO patient, expand the phenotype as well as genotype spectrum of recessive mutations in the TCIRG1 gene by five novel pathogenic variants. Identifying TCIRG1 gene mutations in Egyptian patients with ARO allowed the provision of proper genetic counseling, carrier detection, and prenatal diagnosis starting with two families included herein. It also could pave the way to modern genomic therapeutic approaches.
Leukocyte Tyrosine Kinase (Ltk) Is the Mendelian Determinant of the Axolotl Melanoid Color Variant
Authors: Kabangu Mirindi , Cecil Raissa , Strohl Lloyd , Timoshevskaya Nataliya , Smith Jeramiah J. , Voss Stephen R.
Published: April 2023

The great diversity of color patterns observed among amphibians is largely explained by the differentiation of relatively few pigment cell types during development. Mexican axolotls present a variety of color phenotypes that span the continuum from leucistic to highly melanistic. The melanoid axolotl is a Mendelian variant characterized by large numbers of melanophores, proportionally fewer xanthophores, and no iridophores. Early studies of melanoid were influential in developing the single-origin hypothesis of pigment cell development, wherein it has been proposed that all three pigment cell types derive from a common progenitor cell, with pigment metabolites playing potential roles in directing the development of organelles that define different pigment cell types. Specifically, these studies identified xanthine dehydrogenase (XDH) activity as a mechanism for the permissive differentiation of melanophores at the expense of xanthophores and iridophores. We used bulked segregant RNA-Seq to screen the axolotl genome for melanoid candidate genes and identify the associated locus. Dissimilar frequencies of single-nucleotide polymorphisms were identified between pooled RNA samples of wild-type and melanoid siblings for a region on chromosome 14q. This region contains gephyrin (Gphn), an enzyme that catalyzes the synthesis of the molybdenum cofactor that is required for XDH activity, and leukocyte tyrosine kinase (Ltk), a cell surface signaling receptor that is required for iridophore differentiation in zebrafish. Wild-type Ltk crispants present similar pigment phenotypes to melanoid, strongly implicating Ltk as the melanoid locus. In concert with recent findings in zebrafish, our results support the idea of direct fate specification of pigment cells and, more generally, the single-origin hypothesis of pigment cell development.
Taking Care of an Adolescent and Young Adult Cancer Survivor: A Systematic Review of the Impact of Cancer on Family Caregivers
Authors: Neves Maria Carolina , Bártolo Ana , Prins Judith B. , Sales Célia M. D. , Monteiro Sara
Published: April 2023

Research usually investigates adolescents and young adults (AYA) with cancer in combination with younger and older cancer patients and survivors. However, AYAs with cancer are a unique group, and their caregivers’ experience may also differ from other caregivers of cancer survivors. This systematic review aims to understand the impact of a cancer diagnosis on family caregivers, comparing the experience of caregivers of AYA childhood cancer survivors (AYA CCS) and caregivers of AYA with cancer. Relevant studies were identified through PubMed, Scopus, and Web of Science databases, and their quality was assessed using the Joanna Briggs Institute’s critical appraisal checklists. Sixteen studies (17 reports) met the inclusion criteria. Findings were synthesized separately for caregivers of AYA CCS and caregivers of AYA with cancer. Results showed that caregivers in both groups experienced high distress after the diagnosis. Partners of AYAs with cancer experienced diminished quality of life (QoL) and over half reported moderate to high fear of cancer recurrence (FCR). Findings indicated that cancer negatively impacts family caregivers, regardless of the patient’s age at diagnosis. However, findings are heterogeneous, and most do not focus on QoL or FCR. More research is needed on the impact of cancer among these family caregivers.
Development of an Interprofessional Psychosocial Interventions Framework
Authors: Branjerdporn Grace , Gillespie Kerri Marie , Dymond Alex , Reyes Neil Josen Delos , Robertson Julia , Almeida-Crasto Alice , Bethi Shailendhra
Published: April 2023

To meet the increasingly complex needs of mental health consumers, it is essential for multidisciplinary clinicians to have capabilities across a range of psychosocial interventions. Despite this, there is scant evidence investigating the existing levels of knowledge and skills of specialties within multidisciplinary mental health teams. The purpose of this paper was to describe the self-reported capabilities of mental health clinicians, and to provide a rationale for the Psychosocial Interventions Framework Assessment (PIFA), which aims to enhance the access to, and quality of, evidence-informed practice for consumers of mental health services (MHSs) by strengthening workforce capabilities and leadership for psychosocial therapies. Using the Delphi method, the team developed a 75-item survey based on the 10-point Mental Health Recovery Star (MHRS). Participants completed a self-administered survey indicating their perceived capabilities in the PIFA items. The findings revealed lower-than-expected average scores between ‘novice’ and ‘proficient’, highlighting the need for further development of specific training and education modules for individual teams. This is the first framework of its nature to use the Recovery StarTM to determine the psychosocial areas and domains for the assessment of practitioners’ strengths and needs for skill development.
Population Genetic Diversity of Two Blue Oat Mite Species on Triticum Hosts in China
Authors: Wang Xian , Wang Wenjie , Qin Yang , Wang Mian , Li Yaying , Liu Huai
Published: April 2023

Blue oat mite species, including Penthaleus major and P. tectus, are pests widely distributed across China that cause damage to winter wheat. This study evaluated the genetic diversity of P. major and P. tectus on Triticum hosts collected from 23 geographic locations based on mitochondrial cytochrome c oxidase subunit I (COI) sequences. We identified nine haplotypes in 438 P. major individuals from 21 geographic locations and five haplotypes in 139 P. tectus individuals from 11 geographic locations. Meanwhile, P. major exhibits high values of haplotype diversity (Hd) and nucleotide diversity (Pi) (Hd = 0.534 > 0.5 and Pi = 0.012 > 0.005), representing a large stable population with a long evolutionary history. P. tectus shows low values of Hd and Pi (Hd = 0.112 < 0.5 and Pi = 0 < 0.005), which suggest recent founder events. Moreover, demographic analysis suggested that P. major and P. tectus have not undergone a recent population expansion. The lowest genetic variation was observed in Xiangzhou (XZ-HB), Zaoyang (ZY-HB), Siyang (SY-JS), and Rongxian (RX-SC), with only one species and one haplotype identified in over 30 individuals. Robust genetic differentiation was found in P. major compared to P. tectus, which provides a theoretical basis for the widespread distribution of P. major in China.
Geostatistical Analysis of the Spatial Variation of Chrysolina aeruginosa Larvae at Different Stages in Desert Ecosystems
Authors: He Zeshuai , Chen Liangyue , Yang Ying , Zhao Fuqiang , Zhou Chunmei , Zhang Dazhi
Published: April 2023

Chrysolina aeruginosa is a major pest of Artemisia ordosica, and knowledge of the spatial distribution pattern of its larvae in their natural habitat is crucial for the implementation of effective control measures. This study employed geostatistical methods to investigate the damage caused by larvae of different age groups and their spatial distribution pattern. The distribution of C. aeruginosa larvae, which cause damage to A. ordosica, differed significantly according to their age. Younger larvae were predominantly found in the middle and upper parts of the plant, whereas older larvae were mainly distributed in the middle and lower parts, with significant differences in distribution location. A generalized linear model analysis revealed that the height of the plant, and plant morphological characteristics such as height, crown width, and ground diameter were significantly correlated with the number of larvae present. Furthermore, the interaction of age with other variables had an impact on the number of larvae. Kriging interpolation showed that C. aeruginosa larvae were distributed in aggregated patches with strong spatial heterogeneity. The younger larvae were more abundant in the center of the sample site, while the older larvae tended to be distributed toward the edges. These findings provide valuable information for designing effective control programs.
A DDoS Attack Detection Method Using Conditional Entropy Based on SDN Traffic
Authors: Tian Qiwen , Miyata Sumiko
Published: April 2023

To detect each network attack in an SDN environment, an attack detection method is proposed based on an analysis of the features of the attack and the change in entropy of each parameter. Entropy is a parameter used in information theory to express a certain degree of order. However, with the increasing complexity of networks and the diversity of attack types, existing studies use a single entropy, which does not discriminate correctly between attacks and normal traffic and may lead to false positives. In this paper, we propose new state determination standards that use the normal distribution characteristics of the entropy value at the time which an attack did not occur, subdivide the normal and abnormal range represented by the entropy value, improving the accuracy of attack determination. Furthermore, we show the effectiveness of the proposed method by numerical analysis.
Therapeutic Strategies to Prevent the Recurrence of Nasal Polyps after Surgical Treatment: An Update and In Vitro Study on Growth Inhibition of Fibroblasts
Authors: Rizzi Angela , Gammeri Luca , Cordiano Raffaele , Valentini Mariagrazia , Centrone Michele , Marrone Sabino , Inchingolo Riccardo , Lohmeyer Franziska Michaela , Cavaliere Carlo , Ria Francesco , Cadoni Gabriella , Gangemi Sebastiano , Nucera Eleonora
Published: April 2023

Chronic rhinosinusitis with nasal polyps (CRSwNP) is the most bothersome phenotype of chronic rhinosinusitis, which is typically characterized by a Type 2 inflammatory reaction, comorbidities and high rates of nasal polyp recurrence, causing severe impact on quality of life. Nasal polyp recurrence rates, defined as the number of patients undergoing revision endoscopic sinus surgery, are 20% within a 5 year period after surgery. The cornerstone of CRSwNP management consists of anti-inflammatory treatment with local corticosteroids. We performed a literature review regarding the therapeutic strategies used to prevent nasal polyp recurrence after surgical treatment. Finally, we report an in vitro study evaluating the efficacy of lysine–acetylsalicylic acid and other non-steroidal anti-inflammatory drugs (ketoprofen and diclofenac) on the proliferation of fibroblasts, obtained from nasal polyp tissue samples. Our study demonstrates that diclofenac, even more so than lysine–acetylsalicylic acid, significantly inhibits fibroblast proliferation and could be considered a valid therapeutic strategy in preventing CRSwNP recurrence.
A Genetic-Algorithm-Based Approach for Optimizing Tool Utilization and Makespan in FMS Scheduling
Authors: Grassi Andrea , Guizzi Guido , Popolo Valentina , Vespoli Silvestro
Published: April 2023

This paper proposes a genetic algorithm approach to solve the identical parallel machines problem with tooling constraints in job shop flexible manufacturing systems (JS-FMSs) with the consideration of tool wear. The approach takes into account the residual useful life of tools and allocates a set of jobs with specific processing times and tooling requirements on identical parallel machines. Two metrics are introduced to evaluate the scheduling decisions and optimize the scheduling process, with the competitive goal of maximizing tool utilization and minimizing production makespan. The proposed approach searches for a set of optimal solutions on the Pareto front that offers the best possible balance between these two objectives, achieving optimal local performance in terms of both makespan and tool utilization. The approach is implemented with a customized genetic algorithm and validated on a real case study from a company operating in the aerospace sector, which confirms its effectiveness in increasing tool utilization and reducing the makespan. The results show that the proposed approach has significant practical implications for the manufacturing industry, particularly in the production of high-value materials such as those in the aerospace sector that require costly tools. This paper contributes to the operational research community by providing advanced scheduling algorithms that can optimize both the makespan and the tool utilization concurrently, improving production efficiency and maintaining competitiveness in the manufacturing industry.
Role of Endemism and Other Factors in Determining the Introduction Success of Rare and Threatened Species in Tashkent Botanical Garden
Authors: Volis Sergei , Belolipov Igor V. , Asatulloev Temur , Turgunov Mirabdulla
Published: April 2023

Although rare and threatened species are maintained in many botanical gardens around the world, detailed reports on the success or failure of their introduction appear infrequently, which makes it difficult to understand the major constraints of growing imperilled species in botanical garden living collections. Though intuitively, a level of endemism appears to be important, its role as a predictor of species cultivation success in the garden living collections has never been tested. This paper summarizes the experience of the Tashkent Botanical Garden in creating and maintaining living collections of rare and threatened species of Uzbekistan, trying to understand the role of endemism and other factors in the success and failure of these species cultivation. We found that out of 100 rare and threatened species introduced, the cultivation of 26 failed. Most of these species were endemic to the country, occupying soil types and habitats different from those of the garden site. However, surprisingly, the introduction of many analogous species has been successful. This implies that some narrow endemics can be successfully grown in botanical gardens, but to predict which can and which cannot is impossible, and there are no alternatives to introduction trials. Overall, the large number of rare and threatened species for which introductions were successful confirms the important role of ex situ conservation in preserving critically endangered biodiversity and should stimulate further work in this direction. The future efforts of the garden staff will focus on two major objectives: (i) collecting seeds of endangered species that have so far skipped attention or their collection missions have not been successful; and (ii) propagating those species that have proven cultivation success and using the propagated material for in situ actions.
Flogomicina: A Natural Antioxidant Mixture as an Alternative Strategy to Reduce Biofilm Formation
Authors: Amante Chiara , De Soricellis Chiara , Luccheo Gianni , Luccheo Luigi , Russo Paola , Aquino Rita Patrizia , Del Gaudio Pasquale
Published: April 2023

The National Institute of Health has reported that approximately 80% of chronic infections are associated with biofilms, which are indicated as one of the main reasons for bacteria’s resistance to antimicrobial agents. Several studies have revealed the role of N-acetylcysteine (NAC), in reducing biofilm formation induced by different microorganisms. A novel mixture made up of NAC and different natural ingredients (bromelain, ascorbic acid, Ribes nigrum, resveratrol, and pelargonium) has been developed in order to obtain a pool of antioxidants as an alternative strategy for biofilm reduction. The study has demonstrated that the mixture is able to significantly enhance NAC activity against different Gram-positive and Gram-negative bacteria. It has shown an increase in NAC permeation in vitro through an artificial fluid, moving from 2.5 to 8 μg/cm2 after 30 min and from 4.4 to 21.6 μg/cm2 after 180 min, and exhibiting a strongly fibrinolytic activity compared to the single components of the mixture. Moreover, this novel mixture has exhibited an antibiofilm activity against S aureus and the ability to reduce S. aureus growth by more than 20% in a time-killing assay, while on E. coli, and P. mirabilis, the growth was reduced by more than 80% compared to NAC. The flogomicina mixture has also been proven capable of reducing bacterial adhesion to abiotic surfaces of E.coli, by more than 11% concerning only the NAC. In combination with amoxicillin, it has been shown to significantly increase the drug’s effectiveness after 14 days, offering a safe and natural way to reduce the daily dosage of antibiotics in prolonged therapies and consequently, reduce antibiotic resistance.
Applications of Industry 4.0 Technologies in Warehouse Management: A Systematic Literature Review
Authors: Tikwayo Lihle N. , Mathaba Tebello N. D.
Published: April 2023

Background: Recent literature indicates that warehouse management costs account for a significant portion of overall logistics costs in companies. Warehousing requires the classification, controlling and management of inventory as well as processing of related information. Therefore, adopting efficient and reasonable warehouse management measures to achieve effective management and control of materials is a key means to flexibly adjusting the supply and demand of storage materials and reduce operating costs. There remains a gap in the understanding of benefits and barriers to the full adoption of Industry 4.0 technologies and decision support systems (DSSs) in warehouse management. Methods: This work applies a systematic literature review methodology of recent implementation case studies to analyze documented barriers and benefits of Industry 4.0 technology adoption in warehouse management. For analysis, benefits and barriers are ranked in order of importance using Pareto analysis based on their frequency of occurrence. Results: Improved process efficiency, the availability of real-time data, added competitive advantage and the ability to integrate business activities digitally are the top four most important benefits of implementing Industry 4.0 technologies and decision support systems in warehouse management. The prominent barriers to implementation are high life cycle cost, challenging physical environment/layout, inadequate supporting resource constraints, increased security risk and high energy consumption. Conclusions: Barriers to implementing Industry 4.0 technologies are interrelated in nature and prevent businesses from realizing the full benefit of implemented Industry 4.0 technologies. Adequate financial support, new knowledge and skills are required to be able to ensure the successful implementation of Industry 4.0 in warehousing management.
An Improved Load Distribution Model for Gear Transmission in Thermal Elastohydrodynamic Lubrication
Authors: Lu Ruxin , Tang Wencheng , Huang Qi , Xie Junjie
Published: April 2023

The gear drive generally operates in elastohydrodynamic lubrication (EHL) contacts, and the existence of oil film effectively reduces wear and improves transmission stability. However, little research has been devoted to studying the effect of lubrication characteristics on load distribution of gear transmissions. In order to investigate the coupling effect between the lubrication behavior and load distribution, an analytical load distribution model suitable for EHL contact spur gear pairs is proposed. The non-Newtonian transient thermal EHL solution, flexibility of meshing teeth, structural coupling deformation of the gear body and extended tooth contact are considered in the deformation compatibility condition for iteratively solving the load distribution. A parametric analysis is performed to determine the influence of load torque and rotation speed on load sharing ratio and loaded static transmission error. The transient lubrication behaviors based on the proposed load distribution model is compared with that obtained from the traditional model. A series of comparisons with different models demonstrated the correctness, significance and generality of the present model. The results show that it is necessary to consider the thermal EHL calculation into the iterative solution procedure of load distribution model for EHL contact gear pairs. The proposed model is a useful supplement for an accurate study of thermal EHL characteristics of gear transmissions.
Autoimmune Thyroiditis Shifting from Hashimoto’s Thyroiditis to Graves’ Disease
Authors: Daramjav Narantsatsral , Takagi Junko , Iwayama Hideyuki , Uchino Kaori , Inukai Daisuke , Otake Kazuo , Ogawa Tetsuya , Takami Akiyoshi
Published: April 2023

In 15–20% of cases, Graves’ disease (GD) shifts to Hashimoto’s thyroiditis (HT), while the shift from HT to GD is rare. We present a case of a patient in whom HT shifted to GD, along with a literature review. A 50-year-old woman with myxedema was diagnosed with Hashimoto’s disease due to hypothyroidism and the presence of antibodies against thyroid peroxidase (TPOAb) and thyroglobulin (TgAb); she also had thyroid stimulating antibodies (TSAb) without any signs of GD. Although thyroid hormone replacement therapy improved her thyroid function, 2 months later, hyperthyroidism appeared and did not improve after discontinuation of the replacement therapy. The patient was diagnosed with GD, which improved with antithyroid agent administration. To date, only 50 cases regarding conversion from HT to GD have been reported. The median age is 44 years (range, 23–82 years), and the median time of conversion is 7 years (range, 0.1–27 years). The male-to-female ratio of HT conversion to GD is 1:9, closer to that of regular GD (1:10) than that of general HT (1:18). All patients received thyroid hormone replacement therapy for hypothyroidism due to HT. Continuous evaluation of TSAb levels is recommended in HT, particularly in cases of TSAb-positive and those under replacement, since it may help predict conversion to GD. Evaluating the clinical characteristics of patients with HT preceding GD is crucial to ensure appropriate treatment and reduce the risk of adverse events.
Study of Innovative GO/PBI Composites as Possible Proton Conducting Membranes for Electrochemical Devices
Authors: Di Virgilio Matteo , Basso Peressut Andrea , Pontoglio Angelo , Latorrata Saverio , Dotelli Giovanni
Published: April 2023

The appeal of combining polybenzimidazole (PBI) and graphene oxide (GO) for the manufacturing of membranes is increasingly growing, due to their versatility. Nevertheless, GO has always been used only as a filler in the PBI matrix. In such context, this work proposes the design of a simple, safe, and reproducible procedure to prepare self-assembling GO/PBI composite membranes characterized by GO-to-PBI (X:Y) mass ratios of 1:3, 1:2, 1:1, 2:1, and 3:1. SEM and XRD suggested a homogenous reciprocal dispersion of GO and PBI, which established an alternated stacked structure by mutual π-π interactions among the benzimidazole rings of PBI and the aromatic domains of GO. TGA indicated a remarkable thermal stability of the composites. From mechanical tests, improved tensile strengths but worsened maximum strains were observed with respect to pure PBI. The preliminary evaluation of the suitability of the GO/PBI X:Y composites as proton exchange membranes was executed via IEC determination and EIS. GO/PBI 2:1 (IEC: 0.42 meq g−1; proton conductivity at 100 °C: 0.0464 S cm−1) and GO/PBI 3:1 (IEC: 0.80 meq g−1; proton conductivity at 100 °C: 0.0451 S cm−1) provided equivalent or superior performances with respect to similar PBI-based state-of-the-art materials.
Assessing Cast Aluminum Alloys with Computed Tomography Defect Metrics: A Gurson Porous Plasticity Approach
Authors: Gul Armağan , Aslan Ozgur , Kayali Eyüp Sabri , Bayraktar Emin
Published: April 2023

Aluminum alloys have inherent tendencies to produce casting defects caused by alloying or metal melt flow inside the mold. The traditional detection method for these defects includes reduced pressure tests, which assess metal quality in a destructive manner. This leaves a gap between metal quality assessments and tensile test correlations. Computed tomography (CT) scans offer crucial assistance in evaluating the internal quality of castings without damaging the structure. This provides a valuable opportunity to couple mechanical tests with numerical methods such as finite element analysis to predict the mechanical performance of the alloy. The present study aims to evaluate the internal quality of cast aluminum alloys using CT scans and to correlate the defect metrics obtained from CT scans with mechanical test results. The Gurson-type material model and finite element methodology have been used to validate the correlation studies. Therefore, we propose a more holistic approach to predicting the behavior of metals by coupling damage models with CT scans and mechanical tests. The study investigates several CT metrics such as the defect volume, total defect surface, biggest defect surface, and projected area of defects. The conclusion reveals that CT scans provide crucial assistance in evaluating the internal quality of castings, and CT defect metrics can be used to build correlations between mechanical tests and CT evaluations. The study also suggests that the concept of adjusted representative material yield parameter (ARMY) or computed representative material yield parameter (CRMY) can be used to correlate CT metrics with mechanical strength in cast materials and parts for a given aluminum alloy. Overall, the study proposes a more comprehensive methodology to assess the quality of cast aluminum alloys and couple the quality to mechanical performance.
Shear Transformation Zone and Its Correlation with Fracture Characteristics for Fe-Based Amorphous Ribbons in Different Structural States
Authors: Dong Weiwei , Dong Minshuai , Qian Danbo , Zhang Jiankang , Zhu Shigen
Published: April 2023

Fe-based amorphous alloys often exhibit severe brittleness induced by annealing treatment, which increases the difficulties in handling and application in the industry. In this work, the shear transformation zone and its correlation with fracture characteristics for FeSiB amorphous alloy ribbons in different structural states were investigated. The results show that the bending strain decreases sharply with the annealing temperature increase, accompanied by decreased shear band density and the induced plastic deformation zone. Furthermore, the microscopic fracture surface features transform from a micron-scale dimple pattern to nano-scale dimples and periodic corrugations. According to nano-indentation results, the strain rate sensitivity and shear transformation zone volume change significantly upon annealing treatment, which is responsible for the deterioration of bending ductility and the transition of microscopic fracture surface features.
Mycotoxin Contamination Status of Cereals in China and Potential Microbial Decontamination Methods
Authors: Zhang Jing , Tang Xi , Cai Yifan , Zhou Wen-Wen
Published: April 2023

The presence of mycotoxins in cereals can pose a significant health risk to animals and humans. China is one of the countries that is facing cereal contamination by mycotoxins. Treating mycotoxin-contaminated cereals with established physical and chemical methods can lead to negative effects, such as the loss of nutrients, chemical residues, and high energy consumption. Therefore, microbial detoxification techniques are being considered for reducing and treating mycotoxins in cereals. This paper reviews the contamination of aflatoxins, zearalenone, deoxynivalenol, fumonisins, and ochratoxin A in major cereals (rice, wheat, and maize). Our discussion is based on 8700 samples from 30 provincial areas in China between 2005 and 2021. Previous research suggests that the temperature and humidity in the highly contaminated Chinese cereal-growing regions match the growth conditions of potential antagonists. Therefore, this review takes biological detoxification as the starting point and summarizes the methods of microbial detoxification, microbial active substance detoxification, and other microbial inhibition methods for treating contaminated cereals. Furthermore, their respective mechanisms are systematically analyzed, and a series of strategies for combining the above methods with the treatment of contaminated cereals in China are proposed. It is hoped that this review will provide a reference for subsequent solutions to cereal contamination problems and for the development of safer and more efficient methods of biological detoxification.
PdxNiy/TiO2 Electrocatalysts for Converting Methane to Methanol in An Electrolytic Polymeric Reactor—Fuel Cell Type (PER-FC)
Authors: Coelho Jéssica F. , Gutierrez Isabely M. , Filho Nivaldo G. P. , Zambiazi Priscilla J. , Neto Almir O. , de Souza Rodrigo F. B.
Published: April 2023

PdxNiy/TiO2 bimetallic electrocatalysts were used in fuel cell polymeric electrolyte reactors (PER-FC) to convert methane into methanol through the partial oxidation of methane promoted by the activation of water at room temperature. X-ray diffraction measurements showed the presence of Pd and Ni phases and TiO2 anatase phase. TEM images revealed mean particle sizes larger than those reported for PdNi materials supported, indicating that TiO2 promotes particle aggregation on its surface. Information on the surface structure of electrocatalysts obtained by Raman spectra indicated the presence or formation of NiO. The PER-FC tests showed the highest power density for the electrocatalyst with the lowest amount of nickel Pd80Ni20/TiO2 (0.58 mW cm−2). The quantification of methanol through the eluents collected from the reactor showed higher concentrations of methanol produced, revealing that the use of TiO2 as a support also increased the reaction rate.
Selenized Chickpea Sprouts Hydrolysates as a Potential Anti-Aging Ingredient
Authors: Serrano-Sandoval Sayra N. , Jiménez-Rodríguez Antonio , Hernández-Pérez Jesús , Chavez-Santoscoy Rocio Alejandra , Guardado-Félix Daniela , Antunes-Ricardo Marilena
Published: April 2023

Skin aging represents a health and aesthetic problem that could result in infections and skin diseases. Bioactive peptides can potentially be used in skin aging regulation. Chickpea (Cicer arietinum L.) selenoproteins were obtained from germination with 2 mg Na2SeO3/100 g of seeds for 2 days. Alcalase, pepsin, and trypsin were used as hydrolyzers, and a membrane < 10 kDa was used to fractionate the hydrolysate. Se content, antioxidant capacity, elastase and collagen inhibition, functional stability, and preventative capacity were analyzed. Significant increases in Se content were found in germinated chickpea flour and protein related to the control. An increase of 38% in protein was observed in the selenized flour related to the control. A band (600–550 cm−1) observed in the selenized hydrolysates suggested the insertion of Se into the protein. Hydrolysates from pepsin and trypsin had the highest antioxidant potential. Se enhanced the stability of total protein and protein hydrolysates through time and increased their antioxidant capacity. Hydrolysates > 10 kDa had higher elastase and collagenase inhibition than the total protein and hydrolysates < 10 kDa. Protein hydrolysates < 10 kDa 6 h before UVA radiation had the highest inhibition of collagen degradation. Selenized protein hydrolysates showed promising antioxidant effects that could be related to skin anti-aging effects.
Optimization of Pulsed Laser Ablation and Radio-Frequency Sputtering Tandem System for Synthesis of 2D/3D Al2O3-ZnO Nanostructures: A Hybrid Approach to Synthesis of Nanostructures for Gas Sensing Applications
Authors: Labis Joselito Puzon , Albrithen Hamad A. , Hezam Mahmoud , Ali Shar Muhammad , Algarni Ahmad , Alhazaa Abdulaziz N. , El-Toni Ahmed Mohamed , Alduraibi Mohammad Abdulaziz
Published: April 2023

In this paper, a unique hybrid approach to design and synthesize 2D/3D Al2O3-ZnO nanostructures by simultaneous deposition is presented. Pulsed laser deposition (PLD) and RF magnetron sputtering (RFMS) methods are redeveloped into a single tandem system to create a mixed-species plasma to grow ZnO nanostructures for gas sensing applications. In this set-up, the parameters of PLD have been optimized and explored with RFMS parameters to design 2D/3D Al2O3-ZnO nanostructures, including nanoneedles/nanospikes, nanowalls, and nanorods, among others. The RF power of magnetron system with Al2O3 target is explored from 10 to 50 W, while the ZnO-loaded PLD’s laser fluence and background gases are optimized to simultaneously grow ZnO and Al2O3-ZnO nanostructures. The nanostructures are either grown via 2-step template approach, or by direct growth on Si (111) and MgO<0001> substrates. In this approach, a thin ZnO template/film was initially grown on the substrate by PLD at ~300 °C under ~10 milliTorr (1.3 Pa) O2 background pressure, followed by growth of either ZnO or Al2O3-ZnO, using PLD and RFMS simultaneously under 0.1–0.5 Torr (13–67 Pa), and Ar or Ar/O2 background in the substrate temperate range of 550–700 °C. Growth mechanisms are then proposed to explain the formation of Al2O3-ZnO nanostructures. The optimized parameters from PLD-RFMS are then used to grow nanostructures on Au-patterned Al2O3-based gas sensor to test its response to CO gas from 200 to 400 °C, and a good response is observed at ~350 °C. The grown ZnO and Al2O3-ZnO nanostructures are quite exceptional and remarkable and have potential applications in optoelectronics, such in bio/gas sensors.
Use of Nickel Oxide Catalysts (Bunsenites) for In-Situ Hydrothermal Upgrading Process of Heavy Oil
Authors: Alonso Jiménez Padilla Pedro , Djimasbe Richard , Zairov Rustem , Yuan Chengdong , Al-Muntaser Ameen A. , Stepanov Alexey , Nizameeva Guliya , Dovzhenko Alexey , Suwaid Muneer A. , Varfolomeev Mikhail A. , Zinnatullin Almaz L.
Published: April 2023

In this study, Nickel oxide-based catalysts (NixOx) were synthesized and used for the in-situ upgrading process of heavy crude oil (viscosity 2157 mPa·s, and API gravity of 14.1° at 25 °C) in aquathermolysis conditions for viscosity reduction and heavy oil recovery. All characterizations of the obtained nanoparticles catalysts (NixOx) were performed through Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), X-Ray and Diffraction (XRD), and ASAP 2400 analyzer from Micromeritics (USA), methods. Experiments of catalytic and non-catalytic upgrading processes were carried out in a discontinuous reactor at a temperature of 300 °C and 72 bars for 24 h and 2% of catalyst ratio to the total weight of heavy crude oil. XRD analysis revealed that the use of nanoparticles of NiO significantly participated in the upgrading processes (by desulfurization) where different activated form catalysts were observed, such as α-NiS, β-NiS, Ni3S4, Ni9S8, and NiO. The results of viscosity analysis, elemental analysis, and 13C NMR analysis revealed that the viscosity of heavy crude oil decreased from 2157 to 800 mPa·s, heteroatoms removal from heavy oil ranged from S—4.28% to 3.32% and N—0.40% to 0.37%, and total content of fractions (ΣC8–C25) increased from 59.56% to a maximum of 72.21%, with catalyst-3 thank to isomerization of normal and cyclo-alkanes and dealkylation of lateral chains of aromatics structures, respectively. Moreover, the obtained nanoparticles showed good selectivity, promoting in-situ hydrogenation-dehydrogenation reactions, and hydrogen redistribution over carbons (H/C) is improved, ranging from 1.48 to a maximum of 1.77 in sample catalyst-3. On the other hand, the use of nanoparticle catalysts have also impacted the hydrogen production, where the H2/CO provided from the water gas shift reaction has increased. Nickel oxide catalysts have the potential for in-situ hydrothermal upgrading of heavy crude oil because of their great potential to catalyze the aquathermolysis reactions in the presence of steam.
Emerging Two-Dimensional Materials: Inspiring Nanotechnologies for Smart Energy Management
Authors: Bonera Emiliano , Molle Alessandro
Published: April 2023

Two-dimensional (2D) materials are a class of materials that can be reduced to a thickness of a few layers, exhibiting peculiar and innovative properties relative to their three-dimensional solid counterparts [...]
Good Practices for Scientific Article Writing with ChatGPT and Other Artificial Intelligence Language Models
Authors: Castellanos-Gomez Andres
Published: April 2023

The release of ChatGPT by OpenAI in late November 2022 has shaken the foundations of many industries based in content generation and writing [...]
Sarcopenia and Cognitive Decline in Older Adults: Targeting the Muscle–Brain Axis
Authors: Arosio Beatrice , Calvani Riccardo , Ferri Evelyn , Coelho-Junior Hélio José , Carandina Angelica , Campanelli Federica , Ghiglieri Veronica , Marzetti Emanuele , Picca Anna
Published: April 2023

Declines in physical performance and cognition are commonly observed in older adults. The geroscience paradigm posits that a set of processes and pathways shared among age-associated conditions may also serve as a molecular explanation for the complex pathophysiology of physical frailty, sarcopenia, and cognitive decline. Mitochondrial dysfunction, inflammation, metabolic alterations, declines in cellular stemness, and altered intracellular signaling have been observed in muscle aging. Neurological factors have also been included among the determinants of sarcopenia. Neuromuscular junctions (NMJs) are synapses bridging nervous and skeletal muscle systems with a relevant role in age-related musculoskeletal derangement. Patterns of circulating metabolic and neurotrophic factors have been associated with physical frailty and sarcopenia. These factors are mostly related to disarrangements in protein-to-energy conversion as well as reduced calorie and protein intake to sustain muscle mass. A link between sarcopenia and cognitive decline in older adults has also been described with a possible role for muscle-derived mediators (i.e., myokines) in mediating muscle–brain crosstalk. Herein, we discuss the main molecular mechanisms and factors involved in the muscle–brain axis and their possible implication in cognitive decline in older adults. An overview of current behavioral strategies that allegedly act on the muscle–brain axis is also provided.
Human Milk Composition and Nutritional Status of Omnivore Human Milk Donors Compared with Vegetarian/Vegan Lactating Mothers
Authors: Ureta-Velasco Noelia , Keller Kristin , Escuder-Vieco Diana , Fontecha Javier , Calvo María V. , Megino-Tello Javier , Serrano José C. E. , Romero Ferreiro Carmen , García-Lara Nadia Raquel , Pallás-Alonso Carmen R.
Published: April 2023

Women of childbearing age in Western societies are increasingly adopting vegetarian diets. These women are sometimes rejected as milk donors, but little about the composition of their milk is known. The present study aimed to compare the intake, nutritional status, and nutritional composition of human milk from omnivore human milk donors (Donors) and vegetarian/vegan lactating mothers (Veg). Milk, blood, and urine samples from 92 Donors and 20 Veg were used to determine their fatty acid profiles, as well as vitamins and minerals. In a representative sample of both groups, we also determined the lipid class profile as a distribution of neutral and polar lipids, the molecular species of triacylglycerols, and the relative composition of phospholipids in their milk. A dietary assessment was conducted with a five-day dietary record (while considering the intake of supplements). We highlight the following results, expressed as the mean (SE), for the Veg vs. Donors: (1) Their docosahexaenoic acid (DHA) intake was 0.11 (0.03) vs. 0.38 (0.03) g/day; the plasma DHA was 0.37 (0.07) vs. 0.83 (0.06)%; and the milk DHA was 0.15 (0.04) vs. 0.33 (0.02)%. (2) Their milk B12 levels were 545.69 (20.49) vs. 482.89 (4.11) pM; 85% of the Veg reported taking B12 supplements (mean dose: 312.1 mcg/day); and the Veg group showed no differences with Donors in terms of total daily intake or plasma B12. (3) Their milk phosphatidylcholine levels were 26.88 (0.67) vs. 30.55 (1.10)%. (4) Their milk iodine levels were 126.42 (13.37) vs. 159.22 (5.13) mcg/L. In conclusion, the Vegs’ milk was shown to be different from the Donors’ milk, mainly due to its low DHA content, which is concerning. However, raising awareness and ensuring proper supplementation could bridge this gap, as has already been achieved for cobalamin.
Optimal Serum 25(OH)D Levels and Vitamin D Intake in Korean Postmenopausal Women
Authors: Shin Hye Ran , Lee Ye Jin , Ly Sun Yung
Published: April 2023

Vitamin D plays a crucial role in regulating the growth and maintenance of the musculoskeletal system. Postmenopausal women are vulnerable to bone fractures because of the decrease in bone mineral density (BMD). Therefore, this study aimed to identify the determinants that influence BMD and the 25(OH)D levels in Korean postmenopausal women. This study collected general and dietary intake information, measured biochemical indices, and conducted BMD tests in 96 postmenopausal women residing in a metropolitan area in Korea. This study analyzed factors that influenced serum 25-hydroxyvitamin D (25(OH)D) and BMD, as well as the correlation between the intact parathyroid hormone (iPTH) and serum 25(OH)D levels. The serum 25(OH)D levels increased by 0.226 ng/mL in the summertime, 0.314 ng/mL in the wintertime, and 0.370 ng/mL on annual average when vitamin D intake rose by 1 µg/1000 kcal. When the serum 25(OH)D levels were ≥18.9 ng/mL, the iPTH levels did not rapidly increase. To maintain the serum 25(OH)D levels at ≥18.9 ng/mL, a daily vitamin D intake of ≥13.21 µg was required. Consequently, consuming vitamin D-fortified foods or vitamin D supplements is necessary to improve both bone health and vitamin D nutritional status.
Fruit Extract, Rich in Polyphenols and Flavonoids, Modifies the Expression of DNMT and HDAC Genes Involved in Epigenetic Processes
Authors: Nowrasteh Ghodratollah , Zand Afshin , Raposa László Bence , Szabó László , Tomesz András , Molnár Richárd , Kiss István , Orsós Zsuzsa , Gerencsér Gellért , Gyöngyi Zoltán , Varjas Tímea
Published: April 2023

Recently, the field of epigenetics has been intensively studied in relation to nutrition. In our study, the gene expression patterns of histone deacetylases (HDACs), which regulate the stability of histone proteins, and DNA methyltransferases (DNMTs), which regulate DNA methylation, were determined in mice. The animals were fed a human-equivalent dose of the aqueous extract of fruit seeds and peels, which is rich in flavonoids and polyphenols, for 28 days and then exposed to the carcinogen 7,12-dimethylbenz(a)anthracene (DMBA). The concentrations of trans-resveratrol and trans-piceid were determined in the consumed extract by HPLC and were 1.74 mg/L (SD 0.13 mg/L) and 2.37 mg/L (SD 0.32 mg/L), respectively, which corresponds to the consumption of 0.2–1 L of red wine, the main dietary source of resveratrol, in humans daily. Subsequently, 24 h after DMBA exposure, the expression patterns of the HDAC and DNMT genes in the liver and kidneys were determined by qRT-PCR. The DMBA-induced expression of the tested genes HDAC1, HDAC2, DNMT1, DNMT3A and DNMT3B was reduced in most cases by the extract. It has already been shown that inhibition of the DNMT and HDAC genes may delay cancer development and tumour progression. We hypothesise that the extract studied may exert chemopreventive effects.