bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanism of Antiradical Activity of Newly Synthesized 4,7-Dihydroxycoumarin Derivatives-Experimental and Kinetic DFT Study
Authors: Milanović Žiko , Dimić Dušan , Žižić Milan , Milenković Dejan , Marković Zoran , Avdović Edina
Published: December 2021

Coumarin derivatives have proven beneficial biological activities, but the mechanism of their radical scavenging potency is not fully understood. In this study, the antiradical capacity of two newly synthesized 4,7-dihydroxycoumarin derivatives: (E)-3-(1-((3-hydroxy-4-methoxyphenyl)amino)-ethylidene)-2,4-dioxochroman-7-yl acetate (A-3OH) and (E)-3-(1-((4-hydroxy-3-methoxyphenyl)amino)ethylidene)-2,4-dioxochroman-7-yl acetate (A-4OH) towards HO• were examined by Electron Paramagnetic Resonance (EPR) Spectroscopy and Density Functional Theory (DFT). The compounds were fully characterized by the elemental microanalysis, IR, and NMR spectroscopies. The effect of pH on the acid–base equilibria is separately discussed and the predominant species at the physiological pH were determined. Several common mechanisms (Hydrogen Atom Transfer (HAT), Single-Electron Transfer followed by Proton Transfer (SET-PT), Sequential Proton Loss followed by Electron Transfer (SPLET), Radical Adduct Formation (RAF), and Intramolecular Hydrogen Atom Abstraction (iHAA)) of radical scavenging were investigated based on thermodynamic and kinetic parameters. EPR results indicated that both compounds significantly reduce the amount of present HO•. The results of the kinetic DFT study demonstrated that both compounds predominantly exhibit antiradical capacity through HAT and SPLET mechanisms. The estimated overall rate constants (koverall) proved that A-4OH shows better antioxidant capacity than A-3OH which is well-correlated with the results obtained by EPR measurement.
Identification, Characterization and Expression Analysis of Anthocyanin Biosynthesis-related bHLH Genes in Blueberry (Vaccinium corymbosum L.)
Authors: Zhang Yongyan , Liu Fan , Wang Bin , Wu Huan , Wu Junwei , Liu Jiapeng , Sun Yueting , Cheng Chunzhen , Qiu Dongliang
Published: December 2021

Basic helix-loop-helix proteins (bHLHs) play very important roles in the anthocyanin biosynthesis of many plant species. However, the reports on blueberry anthocyanin biosynthesis-related bHLHs were very limited. In this study, six anthocyanin biosynthesis-related bHLHs were identified from blueberry genome data through homologous protein sequence alignment. Among these blueberry bHLHs, VcAN1, VcbHLH42-1, VcbHLH42-2 and VcbHLH42-3 were clustered into one group, while VcbHLH1-1 and VcbHLH1-2 were clustered into the other group. All these bHLHs were of the bHLH-MYC_N domain, had DNA binding sites and reported conserved amino acids in the bHLH domain, indicating that they were all G-box binding proteins. Protein subcellular location prediction result revealed that all these bHLHs were nucleus-located. Gene structure analysis showed that VcAN1 gDNA contained eight introns, while all the others contained seven introns. Many light-, phytohormone-, stress- and plant growth and development-related cis-acting elements and transcription factor binding sites (TFBSs) were identified in their promoters, but the types and numbers of cis-elements and TFBSs varied greatly between the two bHLH groups. Quantitative real-time PCR results showed that VcAN1 expressed highly in old leaf, stem and blue fruit, and its expression increased as the blueberry fruit ripened. Its expression in purple podetium and old leaf was respectively significantly higher than in green podetium and young leaf, indicating that VcAN1 plays roles in anthocyanin biosynthesis regulation not only in fruit but also in podetium and leaf. VcbHLH1-1 expressed the highest in young leaf and stem, and the lowest in green fruit. The expression of VcbHLH1-1 also increased as the fruit ripened, and its expression in blue fruit was significantly higher than in green fruit. VcbHLH1-2 showed high expression in stem but low expression in fruit, especially in red fruit. Our study indicated that the anthocyanin biosynthesis regulatory functions of these bHLHs showed certain spatiotemporal specificity. Additionally, VcAN1 might be a key gene controlling the anthocyanin biosynthesis in blueberry, whose function is worth exploring further for its potential applications in plant high anthocyanin breeding.
New Circulating Circular RNAs with Diagnostic and Prognostic Potential in Advanced Colorectal Cancer
Authors: Radanova Maria , Mihaylova Galya , Tasinov Oskan , Ivanova Desislava P. , Stoyanov George St. , Nazifova-Tasinova Neshe , Manev Rostislav , Salim Ayshe , Nikolova Miglena , Ivanova Diana G. , Conev Nikolay , Mihaylova Zhasmina , Donev Ivan
Published: December 2021

Circular RNAs (circRNAs) are a group of special endogenous long non-coding RNAs which are highly stable in the circulation, and, thus, more suitable as new biomarkers of colorectal cancer (CRC). The aim of our study was to explore the plasma expression levels of four circRNAs: has_circ_0001445, hsa_circ_0003028, hsa_circ_0007915 and hsa_circ_0008717 in patients with CRC and to evaluate their associations with clinicopathological characteristics and the clinical outcome of the patients. CircRNAs were extracted from patients’ plasma obtained prior to chemotherapy. Their expression levels were measured by qPCR and calculated applying the 2−ΔΔCt method. The levels of all four circRNAs were significantly increased in the plasma of CRC patients. At the optimal cut-off values hsa_circ_0001445 and hsa_circ_0007915 in plasma could significantly distinguish between patients with or without metastatic CRC with 92.56% sensitivity and 42.86% specificity, and with 86.07% sensitivity and 57.14% specificity, respectively. The mean overall survival (OS) of patients with high/intermediate expression of hsa_circ_0001445 was 30 months, significantly higher in comparison with the mean OS of the patients with low expression—20 months (log-rank test, p = 0.034). In multivariate Cox regression analysis, the low levels of hsa_circ_0001445 were also associated with shorter survival (HR = 1.59, 95% CI: 1.02–2.47, p = 0.040). A prognostic significance of hsa_circ_0001445 for patients with metastatic CRC was established.
Lupinus albus Protein Components Inhibit MMP-2 and MMP-9 Gelatinolytic Activity In Vitro and In Vivo
Authors: Mota Joana , Direito Rosa , Rocha João , Fernandes João , Sepodes Bruno , Figueira Maria Eduardo , Raymundo Anabela , Lima Ana , Ferreira Ricardo Boavida
Published: December 2021

Matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) are regarded as important clinical targets due to their nodal-point role in inflammatory and oncological diseases. Here, we aimed at isolating and characterizing am MMP-2 and-9 inhibitor (MMPI) from Lupinus albus and at assessing its efficacy in vitro and in vivo. The protein was isolated using chromatographic and 2-D electrophoretic procedures and sequenced by using MALDI-TOF TOF and MS/MS analysis. In vitro MMP-2 and 9 inhibitions were determined on colon adenocarcinoma (HT29) cells, as well as by measuring the expression levels of genes related to these enzymes. Inhibitory activities were also confirmed in vivo using a model of experimental TNBS-induced colitis in mice, with oral administrations of 15 mg·kg−1. After chromatographic and electrophoretic isolation, the L. albus MMP-9 inhibitor was found to comprise a large fragment from δ-conglutin and, to a lower extent, small fragments of β-conglutin. In vitro studies showed that the MMPI successfully inhibited MMP-9 activity in a dose-dependent manner in colon cancer cells, with an IC50 of 10 µg·mL−1 without impairing gene expression nor cell growth. In vivo studies showed that the MMPI maintained its bioactivities when administered orally and significantly reduced colitis symptoms, along with a very significant inhibition of MMP-2 and -9 activities. Overall, results reveal a novel type of MMPI in lupine that is edible, proteinaceous in nature and soluble in water, and effective in vivo, suggesting a high potential application as a nutraceutical or a functional food in pathologies related to abnormally high MMP-9 activity in the digestive system.
Juvenile African Clawed Frogs (Xenopus laevis) Express Growth, Metamorphosis, Mortality, Gene Expression, and Metabolic Changes When Exposed to Thiamethoxam and Clothianidin
Authors: Jenkins Jill A. , Hartop Katherine R. , Bukhari Ghadeer , Howton Debra E. , Smalling Kelly L. , Mize Scott V. , Hladik Michelle L. , Johnson Darren , Draugelis-Dale Rassa O. , Brown Bonnie L.
Published: December 2021

Neonicotinoids (NEO) represent the main class of insecticides currently in use, with thiamethoxam (THX) and clothianidin (CLO) primarily applied agriculturally. With few comprehensive studies having been performed with non-target amphibians, the aim was to investigate potential biomarker responses along an adverse outcome pathway of NEO exposure, whereby data were collected on multiple biological hierarchies. Juvenile African clawed frogs, Xenopus laevis, were exposed to commercial formulations of THX and CLO at high (100 ppm) and low (20 ppm) concentrations of the active ingredient. Mortality, growth, development, liver metabolic enzyme activity, and gene expression endpoints were quantified. Tadpoles (n > 1000) from NF 47 through tail resorption stage (NF 66) were exposed to NEO or to NEO-free media treatments. Liver cell reductase activity and cytotoxicity were quantified by flow cytometry. Compared to control reference gene expressions, levels of expression for NEO receptor subunits, cell structure, function, and decontamination processes were measured by RT-qPCR by using liver and brain. Mortality in THX high was 21.5% compared to the control (9.1%); the metabolic conversion of THX to CLO may explain these results. The NF 57 control tadpoles were heavier, longer, and more developed than the others. The progression of development from NF 57–66 was reduced by THX low, and weight gain was impaired. Liver reductases were highest in the control (84.1%), with low NEO exhibiting the greatest reductions; the greatest cytotoxicity was seen with THX high. More transcriptional activity was noted in brains than in livers. Results affirm the utility of a study approach that considers multiple complexities in ecotoxicological studies with non-target amphibians, underscoring the need for simultaneously considering NEO concentration-response relationships with both whole-organism and biomarker endpoints.
The Study of a 231 French Patient Cohort Significantly Extends the Mutational Spectrum of the Two Major Usher Genes MYO7A and USH2A
Authors: Mansard Luke , Baux David , Vaché Christel , Blanchet Catherine , Meunier Isabelle , Willems Marjolaine , Faugère Valérie , Baudoin Corinne , Moclyn Melody , Bianchi Julie , Dollfus Helene , Gilbert-Dussardier Brigitte , Dupin-Deguine Delphine , Bonneau Dominique , Drumare Isabelle , Odent Sylvie , Zanlonghi Xavier , Claustres Mireille , Koenig Michel , Kalatzis Vasiliki , Roux Anne-Françoise
Published: December 2021

Usher syndrome is an autosomal recessive disorder characterized by congenital hearing loss combined with retinitis pigmentosa, and in some cases, vestibular areflexia. Three clinical subtypes are distinguished, and MYO7A and USH2A represent the two major causal genes involved in Usher type I, the most severe form, and type II, the most frequent form, respectively. Massively parallel sequencing was performed on a cohort of patients in the context of a molecular diagnosis to confirm clinical suspicion of Usher syndrome. We report here 231 pathogenic MYO7A and USH2A genotypes identified in 73 Usher type I and 158 Usher type II patients. Furthermore, we present the ACMG classification of the variants, which comprise all types. Among them, 68 have not been previously reported in the literature, including 12 missense and 16 splice variants. We also report a new deep intronic variant in USH2A. Despite the important number of molecular studies published on these two genes, we show that during the course of routine genetic diagnosis, undescribed variants continue to be identified at a high rate. This is particularly pertinent in the current era, where therapeutic strategies based on DNA or RNA technologies are being developed.
Probabilistic Evaluation of the Exploration–Exploitation Balance during the Search, Using the Swap Operator, for Nonlinear Bijective S-Boxes, Resistant to Power Attacks
Authors: Legón-Pérez Carlos Miguel , Menéndez-Verdecía Jorge Ariel , Martínez-Díaz Ismel , Sosa-Gómez Guillermo , Rojas Omar , Veloz-Remache Germania del Roció
Published: December 2021

During the search for S-boxes resistant to Power Attacks, the S-box space has recently been divided into Hamming Weight classes, according to its theoretical resistance to these attacks using the metric variance of the confusion coefficient. This partition allows for reducing the size of the search space. The swap operator is frequently used when searching with a random selection of items to be exchanged. In this work, the theoretical probability of changing Hamming Weight class of the S-box is calculated when the swap operator is applied randomly in a permutation. The precision of these probabilities is confirmed experimentally. Its limit and a recursive formula are theoretically proved. It is shown that this operator changes classes with high probability, which favors the exploration of the Hamming Weight class of S-boxes space but dramatically reduces the exploitation within classes. These results are generalized, showing that the probability of moving within the same class is substantially reduced by applying two swaps. Based on these results, it is proposed to modify/improve the use of the swap operator, replacing its random application with the appropriate selection of the elements to be exchanged, which allows taking control of the balance between exploration and exploitation. The calculated probabilities show that the random application of the swap operator is inappropriate during the search for nonlinear S-boxes resistant to Power Attacks since the exploration may be inappropriate when the class is resistant to Differential Power Attack. It would be more convenient to search for nonlinear S-boxes within the class. This result provides new knowledge about the influence of this operator in the balance exploration–exploitation. It constitutes a valuable tool to improve the design of future algorithms for searching S-boxes with good cryptography properties. In a probabilistic way, our main theoretical result characterizes the influence of the swap operator in the exploration–exploitation balance during the search for S-boxes resistant to Power Attacks in the Hamming Weight class space. The main practical contribution consists of proposing modifications to the swap operator to control this balance better.
Learnable Leaky ReLU (LeLeLU): An Alternative Accuracy-Optimized Activation Function
Authors: Maniatopoulos Andreas , Mitianoudis Nikolaos
Published: December 2021

In neural networks, a vital component in the learning and inference process is the activation function. There are many different approaches, but only nonlinear activation functions allow such networks to compute non-trivial problems by using only a small number of nodes, and such activation functions are called nonlinearities. With the emergence of deep learning, the need for competent activation functions that can enable or expedite learning in deeper layers has emerged. In this paper, we propose a novel activation function, combining many features of successful activation functions, achieving 2.53% higher accuracy than the industry standard ReLU in a variety of test cases.
Short-Term Load Forecasting Based on the Transformer Model
Authors: Zhao Zezheng , Xia Chunqiu , Chi Lian , Chang Xiaomin , Li Wei , Yang Ting , Zomaya Albert Y.
Published: December 2021

From the perspective of energy providers, accurate short-term load forecasting plays a significant role in the energy generation plan, efficient energy distribution process and electricity price strategy optimisation. However, it is hard to achieve a satisfactory result because the historical data is irregular, non-smooth, non-linear and noisy. To handle these challenges, in this work, we introduce a novel model based on the Transformer network to provide an accurate day-ahead load forecasting service. Our model contains a similar day selection approach involving the LightGBM and k-means algorithms. Compared to the traditional RNN-based model, our proposed model can avoid falling into the local minimum and outperforming the global search. To evaluate the performance of our proposed model, we set up a series of simulation experiments based on the energy consumption data in Australia. The performance of our model has an average MAPE (mean absolute percentage error) of 1.13, where RNN is 4.18, and LSTM is 1.93.
Resilience of Railway Transport to Four Types of Natural Hazards: An Analysis of Daily Train Volumes
Authors: Fabella Vigile Marie , Szymczak Sonja
Published: December 2021

A crucial step in measuring the resilience of railway infrastructure is to quantify the extent of its vulnerability to natural hazards. In this paper, we analyze the vulnerability of the German railway network to four types of natural hazards that regularly cause disruptions in German rail operations: floods, mass movements, slope fires, and tree falls. Using daily train traffic data matched with various data on disruptive events, we quantify the extent to which these four types of natural hazard reduce daily train traffic volumes. With a negative binomial count data regression, we find evidence that the track segments of the German railway network are most vulnerable to floods, followed by mass movements and tree-fall events. On average, floods reduce traffic on track segments by 19% of the average daily train traffic, mass movements by 16%, and tree fall by 4%. Moreover, when more than one type of natural hazard affects the track segment on the same day, train traffic on that segment falls by 34% of the average train traffic. Slope fires have an ambiguous and nonrobust effect on train traffic due to the reverse causality due to its triggering factors. This is the first study that attempts to rank different natural hazards according to their impact on railway traffic. The results have implications for the selection of resilience strategy and can help prioritize policy measures.
PbS1−xSex-Quantum-Dot@MWCNT/P3HT Nanocomposites with Tunable Photoelectric Conversion Performance
Authors: Zhu He , Hu Huilin , Ye Minheng , Ye Jinhua , Wang Defa
Published: December 2021

The photoelectric performance of quantum dots (QDs)-based nanocomposites is closely related to the optical properties of QDs, which play a critical role in the optical absorption and separation/transfer of charge carriers. Herein, we report a nanocomposite composed of light absorber PbS1−xSex quantum dots (QDs), electron-conducting multiwalled carbon nanotubes (MWCNTs) and hole-conducting poly-3-hexylthiophene (P3HT) with tunable photoelectric conversion performance. In addition to using the quantization effect, we proposed solid-solution PbS1−xSex QDs (x = 0, 0.25, 0.5, 0.75, 1) for band gap engineering. In particular, we successfully synthesized relatively small (~5.3 nm) and uniform QDs via the hot-injection method by using PbCl2, S/Se powder and environmentally friendly oleylamine (OLA) as the precursors and/or solvent. By increasing the content of Se, the band gap of PbS1−xSex QDs decreased along with the decrease in the conduction band and valence band edges. The suitable energy level alignment enabled the efficient transfer of photoinduced charge carriers, and hence a much higher photoelectric conversion performance of the PbS1−xSex-QD@MWCNT/P3HT nanocomposites than the individual QDs, P3HT, and binary PbS1−xSex-QD@MWCNT, as well as the best performance, was achieved over PbS0.75Se0.25-QD@MWCNT/P3HT.
Revision and Phylogenetic Analysis of the Genus Phonoctonus Stål, 1853 (Heteroptera, Reduviidae, Harpactorinae)
Authors: Bugaj-Nawrocka Agnieszka , Chłond Dominik
Published: December 2021

This paper presents a taxonomic revision and phylogenetic analysis of nine known species of the genus Phonoctonus Stål, 1853, distributed exclusively in the Afrotropical region. The revision and phylogenetic analysis were performed using morphological data. A full redescription for all species is provided, taxonomical problems are clarified, and diagnostic characters are presented and illustrated. Based on the analysis, thirteen species are recognised as valid: Phonoctonus bifasciatus stat. nov., P. caesar, P. elegans, P. fairmairei stat. nov., P. fasciatus, P. grandis, P. immitis stat. rev., P. luridus, P. lutescens, P. nigrofasciatus stat. rev., P. picta stat. nov., P. picturatus stat. rev., and P. principalis. New synonymies are proposed: Phonoctonus nigrofasciatus= P. fasciatus var. poultoni syn. nov., P. picturatus = P. fasciatus var. discalis syn. nov., and P. principalis = P. validus syn. nov. An identification key to separate the species, drawings of dorsal habitus, and distribution maps of all valid species are presented. Performed morphological phylogenetic analyses indicate monophyly of the genus Phonoctonus.
Alientoma, a Dynamic Database for Alien Insects in Greece and Its Use by Citizen Scientists in Mapping Alien Species
Authors: Kalaentzis Konstantinos , Kazilas Christos , Demetriou Jakovos , Koutsoukos Evangelos , Avtzis Dimitrios N. , Georgiadis Christos
Published: December 2021

Invasive alien species have been increasingly acknowledged as a major threat to native biodiversity and ecosystem services, while their adverse impacts expand to human health, society and the economy on a global scale. Insects represent one of the most numerous alien organismic groups, accounting for about one fifth of their total number. In Greece, a large number of alien insects have been identified, currently reaching 469 species. In recent decades, the contribution of citizen science towards detecting and mapping the distribution of alien insects has been steeply increasing. Addressing the need for up-to-date information on alien species as well as encouraging public participation in scientific research, the Alientoma website—derived from “alien” and the Greek word “entoma”, meaning insects, is presented. The website aims towards providing updated information on alien species of insects to the public as well as the scientific community, raising awareness about biological invasions and addressing their distribution and impacts inter alia. By maintaining a dynamic online database alongside a strong social media presence since its launch, Alientoma has attracted individuals mainly from Greece and Cyprus, interacting with the website through a total of 1512 sessions. Alientoma intends to establish a constantly increasing network of citizen scientists and to supplement early detection, monitoring and management efforts to mitigate the adverse impacts of alien insects in Greece.
Confinement in Extruded Nanocomposites Based on PCL and Mesoporous Silicas: Effect of Pore Sizes and Their Influence in Ultimate Mechanical Response
Authors: Díez-Rodríguez Tamara M. , Blázquez-Blázquez Enrique , Antunes Nadine L. C. , Ribeiro Maria do Rosário , Pérez Ernesto , Cerrada María L.
Published: December 2021

In this study, nanocomposites based on polycaprolactone (PCL) and two types of mesoporous silicas, MCM-41 and SBA-15, were attained by melt extrusion. The effect of the silica incorporated within the PCL matrix was observed, firstly, in the morphological characteristics and degradation behavior of the resultant composites. DSC experiments provided information on the existence of confinement in the PCL–SBA-15 materials through the appearance of an additional small endotherm, located at about 25–50 °C, and attributed to the melting of constrained crystallites. Displacement to a slightly lower temperature of this endothermic event was observed in the first heating run of PCL–MCM-41 composites, attributed to the inferior pore size in the MCM-41 particles. Thus, this indicates variations in the inclusion of PCL chains within these two mesostructures with different pore sizes. Real-time variable-temperature small-angle X-ray scattering (SAXS) experiments with synchrotron radiation were crucial to confirm the presence of PCL within MCM-41 and SBA-15 pores. Accurate information was also deduced from these measurements regarding the influence of these two mesoporous MCM-41 and SBA-15 silicas on PCL long spacing. The differences found in these morphological and structural features were responsible for the ultimate mechanical response exhibited by the two sets of PCL nanocomposites, with a considerably higher increase of mechanical parameters in the SBA-15 family.
Polylactic Acid Piezo-Biopolymers: Chemistry, Structural Evolution, Fabrication Methods, and Tissue Engineering Applications
Authors: Farahani Amirhossein , Zarei-Hanzaki Abbas , Abedi Hamid Reza , Tayebi Lobat , Mostafavi Ebrahim
Published: December 2021

Polylactide acid (PLA), as an FDA-approved biomaterial, has been widely applied due to its unique merits, such as its biocompatibility, biodegradability, and piezoelectricity. Numerous utilizations, including sensors, actuators, and bio-application—its most exciting application to promote cell migration, differentiation, growth, and protein–surface interaction—originate from the piezoelectricity effect. Since PLA exhibits piezoelectricity in both crystalline structure and an amorphous state, it is crucial to study it closely to understand the source of such a phenomenon. In this respect, in the current study, we first reviewed the methods promoting piezoelectricity. The present work is a comprehensive review that was conducted to promote the low piezoelectric constant of PLA in numerous procedures. In this respect, its chemistry and structural origins have been explored in detail. Combining any other variables to induce a specific application or to improve any PLA barriers, namely, its hydrophobicity, poor electrical conductivity, or the tuning of its mechanical properties, especially in the application of cardiovascular tissue engineering, is also discussed wherever relevant.
Hippocampal Adaptations to Continuous Aerobic Training: A Functional and Ultrastructural Evaluation in a Young Murine Model
Authors: Cariati Ida , Bonanni Roberto , Pallone Gabriele , Scimeca Manuel , Frank Claudio , Tancredi Virginia , D’Arcangelo Giovanna
Published: December 2021

Aerobic training is known to influence cognitive processes, such as memory and learning, both in animal models and in humans. Particularly, in vitro and in vivo studies have shown that aerobic exercise can increase neurogenesis in the dentate gyrus, improve hippocampal long-term potentiation (LTP), and reduce age-related decline in mnemonic function. However, the underlying mechanisms are not yet fully understood. Based on this evidence, the aim of our study was to verify whether the application of two aerobic training protocols, different in terms of speed and speed variation, could modulate synaptic plasticity in a young murine model. Therefore, we assessed the presence of any functional changes by extracellular recordings in vitro in mouse hippocampal slices and structural alterations by transmission electron microscopy (TEM). Our results showed that an aerobic training protocol, well designed in terms of speed and speed variation, significantly contributes to improving synaptic plasticity and hippocampal ultrastructure, optimizing its benefits in the brain. Future studies will aim to clarify the underlying biological mechanisms involved in the modulation of synaptic plasticity induced by aerobic training.
Methodology for the Automated Visual Detection of Bird and Bat Collision Fatalities at Onshore Wind Turbines
Authors: Happ Christof , Sutor Alexander , Hochradel Klaus
Published: December 2021

The number of collision fatalities is one of the main quantification measures for research concerning wind power impacts on birds and bats. Despite being integral in ongoing investigations as well as regulatory approvals, the state-of-the-art method for the detection of fatalities remains a manual search by humans or dogs. This is expensive, time consuming and the efficiency varies greatly among different studies. Therefore, we developed a methodology for the automatic detection using visual/near-infrared cameras for daytime and thermal cameras for nighttime. The cameras can be installed in the nacelle of wind turbines and monitor the area below. The methodology is centered around software that analyzes the images in real time using pixel-wise and region-based methods. We found that the structural similarity is the most important measure for the decision about a detection. Phantom drop tests in the actual wind test field with the system installed on 75 m above the ground resulted in a sensitivity of 75.6% for the nighttime detection and 84.3% for the daylight detection. The night camera detected 2.47 false positives per hour using a time window designed for our phantom drop tests. However, in real applications this time window can be extended to eliminate false positives caused by nightly active animals. Excluding these from our data reduced the false positive rate to 0.05. The daylight camera detected 0.20 false positives per hour. Our proposed method has the advantages of being more consistent, more objective, less time consuming, and less expensive than manual search methods.
Overmolding of Hybrid Long and Short Carbon Fiber Polypropylene Composite: Optimizing Processing Parameters
Authors: Budiyantoro Cahyo , Rochardjo Heru S. B. , Nugroho Gesang
Published: December 2021

Injection overmolding was used to produce hybrid unidirectional continuous-short carbon fiber reinforced polypropylene. Polypropylene pellets containing short carbon fibers were melted and overmolded on unidirectional carbon fibers, which act as the core of the composite structure. Four factors were varied in this study: fiber pretension applied to unidirectional fibers, injection pressure, melting temperature, and backpressure used for melting and injecting the composite pellet. This study aimed to evaluate the effect of these factors on fiber volume fraction, flexural strength, and impact strength of the hybrid composite. The relationship between factors and responses was analyzed using Box–Behnken Response Surface Methodology (RSM) and analysis of variance (ANOVA). Each aspect was divided into three levels. There were 27 experimental runs carried out, with three replicated center points. The results showed that the injection molding process parameters had no significant effect on the fiber’s volume fraction. On the other hand, melting temperature and fiber pretension significantly affected impact strength and flexural strength.
Impulse-Based Manufacturing Technologies
Authors: Psyk Verena
Published: December 2021

Modern manufacturing faces extensive technological and economic challenges to remain competitive under the current political and social conditions [...]
Neural Network Based Deep Learning Method for Multi-Dimensional Neutron Diffusion Problems with Novel Treatment to Boundary
Authors: Xie Yuchen , Wang Yahui , Ma Yu , Wu Zeyun
Published: December 2021

In this paper, the artificial neural networks (ANN) based deep learning (DL) techniques were developed to solve the neutron diffusion problems for the continuous neutron flux distribution without domain discretization in advance. Due to its mesh-free property, the DL solution can easily be extended to complicated geometries. Two specific realizations of DL methods with different boundary treatments are developed and compared for accuracy and efficiency, including the boundary independent method (BIM) and boundary dependent method (BDM). The performance comparison on analytic benchmark indicates BDM being the preferred DL method. Novel constructions of trial function are proposed to generalize the application of BDM. For a more in-depth understanding of the BDM on diffusion problems, the influence of important hyper-parameters is further investigated. Numerical results indicate that the accuracy of BDM can reach hundreds of times higher than that of BIM on diffusion problems. This work can provide a new perspective for applying the DL method to nuclear reactor calculations.
MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori
Authors: Hu Congwu , Dong Zhanqi , Deng Boyuan , Wu Qin , Chen Peng , Lu Cheng , Pan Minhui
Published: December 2021

As microRNAs (miRNAs) are important expression regulators of coding RNA, it is important to characterize their role in the interaction between hosts and pathogens. To obtain a comprehensive understanding of the miRNA alternation in Bombyx mori (B. mori) infected with Nosema bombycis (N. bombycis), RNA sequencing and stem-loop qPCR were conducted to screen and identify the significantly differentially expressed miRNAs (DEmiRNAs). A total of 17 such miRNAs were identified in response to N. bombycis infection, among which miR6498-5p efficiently inhibited the proliferation of N. bombycis in BmE-SWU1 (BmE) cells by downregulating pyridoxal phosphate phosphatase 2 (BmPLPP2). In addition, a fluorescence in situ hybridization (FISH) assay showed that miR6498-5p was located in the cytoplasm of BmE cells, while it was not found in the schizonts of N. bombycis. Further investigation of the effect of BmPLPP2 on the proliferation of schizonts found that the positive factor BmPLPP2 could facilitate N. bombycis completing its life cycle in cells by overexpression and RNAi of BmPLPP2. Our findings offer multiple new insights into the role of miRNAs in the interaction between hosts and microsporidia.
Malassezia restricta Pneumonia in Solid Organ Transplant Recipients: First Report of Two Cases
Authors: Mularoni Alessandra , Graziano Elena , Medaglia Alice Annalisa , Buscemi Barbara , Eddens Taylor , Martino Lavinia , Di Carlo Daniele , Cascio Antonio , Conaldi Pier Giulio , Bertani Alessandro , Grossi Paolo Antonio
Published: December 2021

Emerging fungal infections are a major challenge in solid organ transplantation (SOT) and are associated with high morbidity and mortality. We report two cases of Malassezia restricta pneumonia in SOT recipients. Infections were diagnosed with molecular analysis and histology. Patients were treated with antifungal therapy and have fully recovered.
Combination of Strobilurin and Triazole Chemicals for the Management of Blast Disease in Mushk Budji -Aromatic Rice
Authors: Mohiddin Fayaz Ahmad , Bhat Nazir A. , Wani Shabir H. , Bhat Arif H. , Ahanger Mohammad Ashraf , Shikari Asif B. , Sofi Najeebul Rehman , Parveen Shugufta , Khan Gazala H. , Bashir Zaffar , Vachova Pavla , Hassan Sabry , Sabagh Ayman EL
Published: December 2021

Rice blast is considered one of the most important fungal diseases of rice. Although diseases can be managed by using resistant cultivars, the blast pathogen has successfully overcome the single gene resistance in a short period and rendered several varieties susceptible to blast which were otherwise intended to be resistant. As such, chemical control is still the most efficient method of disease control for reducing the losses caused due to diseases. Field experiments were conducted over two successive years, 2018 and 2019, in temperate rice growing areas in northern India. All the fungicides effectively reduced leaf blast incidence and intensity, and neck blast incidence under field conditions. Tricyclazole proved most effective against rice blast and recorded a leaf blast incidence of only 8.41%. Among the combinations of fungicides, azoxystrobin + difenoconazole and azoxystrobin + tebuconazole were highly effective, recording a leaf blast incidence of 9.19 and 10.40%, respectively. The chemical combination mancozeb + carbendazim proved less effective in controlling the blast and it recorded a disease incidence of 27.61%. A similar trend was followed in neck blast incidence with tricyclazole, azoxystrobin + difenoconazole, and azoxystrobin + tebuconazole showing the highest levels of blast reductions. It is evident from the current study that the tested fungicide combinations can be used as alternatives to tricyclazole which is facing the challenges of fungicide resistance development and other environmental concerns and has been banned from use in India and other countries. The manuscript may provide a guideline of fungicide application to farmers cultivating susceptible varieties of rice.
Risk of Vestibulocochlear Disorders in Patients with Migraine or Non-Migraine Headache
Authors: Lee Sang-Hwa , Kim Jong-Ho , Kwon Young-Suk , Lee Jae-June , Sohn Jong-Hee
Published: December 2021

Headaches, especially migraines, have been associated with various vestibular symptoms and syndromes. Tinnitus and hearing loss have also been reported to be more prevalent among migraineurs. However, whether headaches, including migraine or non-migraine headaches (nMH), are associated with vestibular and cochlear disorders remains unclear. Thus, we sought to investigate possible associations between headache and vestibulocochlear disorders. We analyzed 10 years of data from the Smart Clinical Data Warehouse. In patients with migraines and nMH, meniere’s disease (MD), BPPV, vestibular neuronitis (VN) and cochlear disorders, such as sensorineural hearing loss (SNHL) and tinnitus, were collected and compared to clinical data from controls who had health check-ups without headache. Participants included 15,128 with migraines, 76,773 patients with nMH and controls were identified based on propensity score matching (PSM). After PSM, the odds ratios (OR) in subjects with migraine versus controls were 2.59 for MD, 2.05 for BPPV, 2.98 for VN, 1.74 for SNHL, and 1.97 for tinnitus, respectively (p < 0.001). The OR for MD (1.77), BPPV (1.73), VN (2.05), SNHL (1.40), and tinnitus (1.70) in patients with nMH was also high after matching (p < 0.001). Our findings suggest that migraines and nMH are associated with an increased risk of cochlear disorders in addition to vestibular disorders.
Defects in MMR Genes as a Seminal Example of Personalized Medicine: From Diagnosis to Therapy
Authors: Dal Buono Arianna , Gaiani Federica , Poliani Laura , Correale Carmen , Laghi Luigi
Published: December 2021

Microsatellite instability (MSI) is the landmark feature of DNA mismatch repair deficiency, which can be found in 15–20% of all colorectal cancers (CRC). This specific set of tumors has been initially perceived as a niche for geneticists or gastroenterologists focused on inherited predispositions. However, over the years, MSI has established itself as a key biomarker for the diagnosis, then extending to forecasting the disease behavior and prognostication, including the prediction of responsiveness to immunotherapy and eventually to kinase inhibitors, and possibly even to specific biological drugs. Thanks to the contribution of the characterization of MSI tumors, researchers have first acknowledged that a strong lymphocytic reaction is associated with a good prognosis. This understanding supported the prognostic implications in terms of the low metastatic potential of MSI-CRC and has led to modifications in the indications for adjuvant treatment. Furthermore, with the emergence of immunotherapy, this strong biomarker of responsiveness has exemplified the capability of re-activating an effective immune control by removing the brakes of immune evasion. Lately, a subset of MSI-CRC emerged as the ideal target for kinase inhibitors. This therapeutic scenario implies a paradox in which appropriate treatments for advanced disease are effective in a set of tumors that seldom evolve towards metastases.
Applicability of Pharmacogenomically Guided Medication Treatment during Hospitalization of At-Risk Minority Patients
Authors: Saulsberry Loren , Danahey Keith , Middlestadt Merisa , O’Leary Kevin J. , Nutescu Edith A. , Chen Thomas , Lee James C. , Ruhnke Gregory W. , George David , House Larry , van Wijk Xander M. R. , Yeo Kiang-Teck J. , Choksi Anish , Hartman Seth W. , Knoebel Randall W. , Friedman Paula N. , Rasmussen Luke V. , Ratain Mark J. , Perera Minoli A. , Meltzer David O. , O’Donnell Peter H.
Published: December 2021

Known disparities exist in the availability of pharmacogenomic information for minority populations, amplifying uncertainty around clinical utility for these groups. We conducted a multi-site inpatient pharmacogenomic implementation program among self-identified African-Americans (AA; n = 135) with numerous rehospitalizations (n = 341) from 2017 to 2020 (NIH-funded ACCOuNT project/clinicaltrials.gov#NCT03225820). We evaluated the point-of-care availability of patient pharmacogenomic results to healthcare providers via an electronic clinical decision support tool. Among newly added medications during hospitalizations and at discharge, we examined the most frequently utilized medications with associated pharmacogenomic results. The population was predominantly female (61%) with a mean age of 53 years (range 19–86). On average, six medications were newly prescribed during each individual hospital admission. For 48% of all hospitalizations, clinical pharmacogenomic information was applicable to at least one newly prescribed medication. Most results indicated genomic favorability, although nearly 29% of newly prescribed medications indicated increased genomic caution (increase in toxicity risk/suboptimal response). More than one of every five medications prescribed to AA patients at hospital discharge were associated with cautionary pharmacogenomic results (most commonly pantoprazole/suboptimal antacid effect). Notably, high-risk pharmacogenomic results (genomic contraindication) were exceedingly rare. We conclude that the applicability of pharmacogenomic information during hospitalizations for vulnerable populations at-risk for experiencing health disparities is substantial and warrants continued prospective investigation.
Dairy Sheep and Goat Farmers: Socio-Demographic Characteristics and Their Associations with Health Management and Performance on Farms
Authors: Lianou Daphne T. , Fthenakis George C.
Published: December 2021

The objectives were to determine the socio-demographic profiles of small ruminant dairy farmers and to study associations with management practices, production outcomes and health parameters on their farms. In total, 325 sheep flocks and 119 goat herds across Greece were included in the study and visited for collection of information. Socio-demographic characteristics of the dairy farmers and details of management practices, production outcomes and health parameters on their farms were recorded. For the analysis of results, multivariable models were created using mixed-effects logistic regression, with farms as the random effect. Most dairy farmers were male (93.2%), most were full-time professionals in farming (89.4%) and most had a farming family tradition (86.9%). The mean age was 47.0 years and the mean farming experience was 24.3 years. For 17.3% of the farmers, the highest level of education received was primary education, for 54.3% it was secondary education, for 16.4% it was post-secondary vocational training and for 12.0% it was tertiary education. In 35.4% of dairy farms, external farm workers were employed. Of the socio-demographic characteristics, farming experience was associated with geographical location of farms, management system, breed of animals, application of quarantine measures, laboratory evaluation of feedstuffs, ultrasonographic examination for pregnancy diagnosis, application of vaccination against clostridial infections, means of calculation of bodyweight for drug administration to animals, maintenance of colostrum bank, number of annual veterinary visits, annual milk production per animal, number of newborns and somatic cell counts and total bacterial counts in milk. Further, the employment of external farm workers on the farm was associated with management system, machine-milking, number and breed of animals, application of quarantine measures, laboratory evaluation of feedstuffs, ultrasonographic examination for pregnancy diagnosis, number of annual veterinary visits, annual milk production per animal, protein content in milk and number of newborns. The findings indicate that dairy sheep/goat farming is still a family-driven business, but, nevertheless, there are now younger people among these farmers, many of them with post-secondary education. Socio-demographic characteristics may influence the management practices applied, which in turn can have consequences for production and health results of the farms.
Researching the Complexities of the School Subject Swedish as a Second Language: A Linguistic Ethnographic Project in Four Schools
Authors: Hedman Christina , Magnusson Ulrika
Published: December 2021

This article focuses on language education policy for language learners in Sweden by building on a synthesis of findings from a research project on the school subject Swedish as a second language (SSL). The project was located in three upper secondary schools and one primary school with a large proportion of migrant students, of whom a majority studied SSL. We present previously published core findings, as well as revisit data for new analyses. The overarching aim is to contribute new knowledge on the complexities of arranging for sustainable, equitable and high-quality language educational provisions that include the teaching and learning of the language of schooling, through the lens of SSL. First, we outline and discuss the relatively unique design of SSL and discourses surrounding the subject, and also make some international comparisons with English as an Additional Language. Secondly, we discuss the role of pedagogical scaffolding of advanced literacy and literary content, and of multilingual aspects in SSL, as well as examine policy frictions in the data. We conclude by reflecting on the role of teacher competences and research methodology. Researching a second language subject is to stand in the crossroad of macro policy, the theory and practice of language education, and equity. All these aspects need to be considered to reach sustainable educational goals.