bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficiency of utilization of absorbed amino acids in growing lambs given forage and forage: barley diets
Authors: MacRae J. C. , Bruce L. A. , Brown D. S.
Published: September 2010

The efficiency of utilization of absorbed essential amino acids (AA) was studied in wether lambs (35 to 40 kg live weight) given dried grass and dried grass: barley pelleted diets over an intake range from maintenance (M) to 2·5 M energy intake. Each animal was prepared with a duodenal and Heal simple (T-shaped) cannulafor the collection of digesta entering and leaving the small intestine and with a catheter into the abomasum for the infusion of digesta phase markers (103Ru phenanthroline and51Cr ethylene diamine tetra acetic acid). The efficiencies of utilization of total AA and individual essential AA (EAA) were calculated from the ratios of the regressions describing AA retention per unit nitrogen (N) intake (assessed using the N retention data obtained in the present study and the AA composition of N retention derived during an accompanying comparative slaughter experiment) and AA absorption per unit N intake. These ratios for total EAA were 0·5 for the grass diet and 0·59 for the grass plus barley diet. Values for individual EAA ranged from 0·32 for threonine in sheep given the grass diet to 0·88 for arginine in sheep given the grass: barley diet. Whilst the ratios for total and individual EAA were generally higher for the grass: barley diet the very wide 95% confidence limits associated with these derived values make any between-diet or between-EAA comparisons equivocal. The data appear to support the introduction by the Agricultural and Food Research Council (1992), of a scaling factor to reduce the high efficiency of utilization of AA used previously.
The effect of increasing the acidity or osmolality of grass silage by the addition of free or partially neutralized lactic acid on silage intake by sheep and upon osmolality and acid-base balance
Authors: Rooke J. A.
Published: September 2010

A well fermented grass silage was mixed with 9·3 g lactic acid per kg silage supplied either as the free acid (lactic acid) or partially neutralized to pH 5·2 with sodium hydroxide (sodium lactate) to give three dietary treatments. Treatment with lactic acid reduced silage pH (P < 0·05) from 4·03 to 3·80 and increased (P < 0·01) the amount of sodium hydroxide required to raise silage pH to 6·5 (neutralizing value) from 167 to 261 mmol/kg silage and osmolality (P < 0·01) from 712 to 854 mosmoljkg silage; sodium lactate treatment increased (P < 0·05) silage pH to 4·15 and osmolality (P < 0·01) to 964 mosmoljkg silage but did not change neutralizing value.

Wlien fed in a randomized-block design to either six intact lambs or to four rumen fistulated sheep lactic acid treatment reduced (P < 0·05) silage dry matter (DM) intake (g/kg weight0·75) from 34·7 to 27·8 for intact sheep and from 35·5 to 29·9 for fistulated sheep. Intakes of the sodium lactate-treated silage tended to be higher (39·2, intact and 40·1, fistulated sheep, g/kg weight0·75) than the control silage. Silage DM intake was correlated more closely with neutralizing value (r2 = 0·34) than pH (rz = 0·24).

Treatment of silage with lactic acid or sodium lactate did not change rumen pH, volatile fatty acid patterns or osmolality. Blood pH, pCO2 and bicarbonate concentrations were not changed by the diets offered and were within normal ranges. Urine acid-base balance was not affected by lactic acid treatment whereas urine pH, and bicarbonate and sodium excretion were (P < 0·01) increased by sodium lactate treatment of the silage.
Responses in carcass composition to divergent selection for components of efficient lean growth rate in pigs
Authors: Cameron N. D. , Curran M. K.
Published: September 2010

Carcass composition was measured after six generations of divergent selection for lean growth rate on ad-libitum and restricted feeding, lean food conversion and daily food intake in populations of Large White (LW) and Landrace (LR) pigs. There were 161 half-carcass dissections in LW pigs and for LR pigs, a double sampling procedure combined information from 53 half-carcass and 53 hand joint dissections. The performance test started at 30 kg and finished at 85 kg with ad-libitum feeding and after 84 days with restricted feeding, and pigs were slaughtered at the end of the test.

In the LR population, selection for lean growth on restricted feeding increased carcass lean content (605 v. 557 (s.e.d. 19) g/kg), but there were no significant responses in carcass lean content with the selection strategies on adlibitum feeding. Selection for lean food conversion and high lean growth on restricted feeding reduced carcass fat content (201 v. 241 (s.e.d. 14) and 150 v. 218 (s.e.d. 18) g/kg), but selection for high lean growth rate with adlibitum increased carcass fat content (212 v. 185 (s.e.d. 11) g/kg). Responses in carcass composition were not significant with selection on daily food intake.

In the LW population, selection for high lean food conversion or low daily food intake increased carcass lean content (539 v. 494 and 543 v. 477 (s.e.d. 11) g/kg) to a greater extent than selection on lean growth rate (509 v. 475 g/kg). Responses in carcass fat content were equal and opposite to those in carcass lean content. Selection on lean growth rate with ad-libitum feeding increased lean tissue growth rate (LTGR) (491 v. 422 (s.e.d. 23) g/day), but there was no change in fat tissue growth rate (FTGR) (206 v. 217 (s.e.d. 15) g/day). In contrast, FTGR was reduced with selection on lean food conversion (169 v. 225 g/day), but LTGR was not significantly increased (520 v. 482 g/day). Selection for lean growth rate with restricted feeding combined the desirable strategies of lean growth rate on adlibitum feeding and lean food conversion, as LTGR was increased (416 v. 359 (s.e.d. 12) g/day) and FTGR decreased (126 v. 156 (s.e.d. 7) g/day). The preferred selection strategy may be lean growth rate on restricted feeding, which simultaneously emphasizes rate and efficiency of lean growth.

For ad-libitum fed LW pigs, coheritabilities for growth rate, daily food intake and backfat depth with carcass lean content were negative (-0·12, -0·22 and -0·50 (s.e. 0·05), but positive with carcass subcutaneous fat content (0·22, 0·24 and 0·50), when estimated from six generations of performance test data and carcass dissection data in generations 2, 4 and 6.
A comparison of the welfare of sows in different housing conditions
Authors: Broom D. M. , Mendl M. T. , Zanella A. J.
Published: September 2010

Twelve sows in good quality unstrawed stalls, three groups of five sows in strawed pens with individual feeding stalls and sows in a 38-sow group in a strawed yard with an electronic sow feeder were compared during the first four parities. They originated from the same source, were about 9 months of age and in the 7th week of their first pregnancy at the start of the experiment and were kept in adjacent rooms in a building, cared for by the same staff and given the same diets at a rate of 2·2 kg/day per animal. No new animals were added to the groups or stalls during the study and animals returned to the same condition after periods in farrowing and service accommodation. Using a wide range of welfare indicators, it was clear that stall-housed sows had more problems than group-housed sows and that tliese problems were worse in the fourth than in the first pregnancy. By the fourth pregnancy, stall-housed sows spent proportionately 0·14 of time showing activities which were clearly stereotypies and much time on activities which were sometimes stereotyped, i.e. ‘drinking’ and rooting or chewing at pen fittings making a total of proportionately 0·50 of time. Comparable figures for group-housed sows were much lower (0·037 and 0·081 in total). Stall-housed sows were also more aggressive than group-housed by the fourth pregnancy and their body weights were lower. There were no differences using physiological or immunological tests or measures of reproductive output. When the two group-housing systems were compared, sows in the electronic feeder system showed more fighting, especially soon after initial mixing, but fewer total agonistic interactions than sows in groups of five during the first pregnancy. Oral stereotypies were slightly higher in small groups, perhaps because of smaller pen space, than in larger groups but much lower than in stalls. By the fourth pregnancy there were few differences between sows in small and large groups and all seemed to have adapted well to the conditions. Evaluation of welfare in different housing systems requires use of a wide range of measures and of long-term studies.
Utilization of dietary carbohydrates by fish: a comparative study in eel and trout
Authors: García-Gallego M. , Bazoco J. , Suàrez M. D. , Sanz A.
Published: September 2010

To evaluate the ability of the eel to use diets with high carbohydrate content, a comparative study was made by using the rainbow trout, a carnivorous fish, as a reference species. Four isoenergenetic diets (in terms of gross energy) were formulated incorporating different protein/carbohydrate ratios (450/200, 370/300, 290/400 and 210/500 as g/kg dry matter, respectively) and each one was tested in three replicates groups of eels and trout. The data on growth, food conversion and utilization of dietary protein and energy show that the eel has a comparatively greater ability than the rainbow trout for using diets with a high carbohydrate level. Thus it is postulated that higher carbohydrate levels could be used for formulating cheaper diets.
The requirement of the gilthead seabream (Sparus aurata L.) for nicotinic acid
Authors: Morris P. C. , Davies S. J.
Published: September 2010

Semi-purified diets were used to determine the nicotinic acid requirement of the gilthead seabream, Sparus aurata. Vitamin losses during food manufacture were minimal resulting in dietary levels which were close to the declared content. Dietary nicotinic acid contents of 52·21 mg/kg and below resulted in sub-optimum growth, reduced food efficiency and poor values for apparent net protein utilization. Diets containing less than 28·67 mg/kg gave a low proportion of carcass lipid concomitant with an elevation in the relative content of carcass moisture. Although gross deficiency symptoms were not observed, a reduced hepatosomatic index and a slightly lower plasma glucose concentration were detected amongst the fish given diets supplemented with nicotinic acid at the level of 25 mg/kg or less. Based upon the results of broken line analysis and data derived by modelling the weight gain of the fish, the nicotinic acid requirement of Sparus aurata fingerlings lies between 63 and 83 mg/kg of diet or 1·57 to 2·07 µg/kg body weight per day.
The influence of animal age and sward height on the herbage intake and grazing behaviour of Charolais cattle
Authors: Cazcarra R. Ferrer , Petit M.
Published: September 2010

Mature dry cows, 18-month-old heifers and 7-month-old female calves of the Charolais breed (eight animals of each age) were strip-grazed on moderate (15cm) or tall (52 and 32cm) cocksfoot swards over two 15-day experimental periods. The swards were leafy regrowths of 2 (short) and 8 (tall) weeks of age. Herbage intake was estimated by the alkane method, and grazing behaviour was visually recorded.

Bite weight was lower, grazing time and biting rate greater, and organic matter in take of grass (OMIG) higher on shorter swards, the latter being related to a higher in vitro digestibility and nitrogen concentration. Average OMIG per kg live weight (M) was 15·2, 19·1 and 17·0 (s.e.d. 0·49)g in cows, heifers and calves respectively. The average allometric coefficient between OMIG and M was 0·88 (similar between swards), and close to that obtained with hay after the grazing trial.

Faecal markers suggested a greater selectivity of calves compared with older cattle. Average bite weights (BW) were 310 (s.e. 40), 467 (s.e. 112) and 718 (s.e. 144)mg OM in calves, heifers and cows respectively. The allometric coefficient between BW and M was higher in short swards than in tall ones. Biting rates increased and grazing times tended to decrease with age of cattle.
Relationship between rank order and productive parameters in Verata goats during milking
Authors: Patón D. , Martín L. , Cereijo M. , Rota A. , Rojas A. , Tovar J.
Published: September 2010

The hierarchic behaviour of 71 goats of the Verata breed from two independent flocks on two different farms (no.2 = 34 and no. 2 = 37) was studied during the milking-period of a year. Two statistical procedures: canonical correspondence analysis (CCA) and a log-linear model were used. In the CCA, the total frequencies of each goat in each of the 12 positions at the milking stalls during 210 days of lactation were computed as original variables. Canonical variables were the average values of dairy milk production, proportion of protein and proportion of fat. The first two CCA axes accounted for proportionately 0·72 of the variance. The first axis showed the effect of rank order (V1 = 0·45) and the second axis the variability between animals for the same group of hierarchy (V2 = 0·27). The first position (AC1, = 0·58; AC2 = 0·23) and the last position (AC1 = 0·11; AC2 = 0·26) of the milking stalls were in absolute contributions the most significant in the distribution of the four observed groups of hierarchy, as defined by their frequency of access to the first position. Two parameters were highly correlated with both axes: milk protein content (r1, = - 0·8; r2 = 0·9) and the day milk production (r1 = 0·6; r2 = 0·5). These results showed a direct relationship between rank order and milk production, whereas the variability in each group was best defined by the protein content. The log-linear model showed that goats of lactation numbers one, two and three have a similar behaviour with respect to their locations at the milking stalls, being distributed more randomly (â1, = 0·68 < â2 = 0·7 < â3 = 0·71) than goats of a lactation number four (â4 = 2·95). This effect could be a consequence of two different but related factors: either the animals develop a preference for a particular position over time, or that animals in their fourth lactation showed a more stable behaviour pattern which affected their body condition.
Autumnal timing of photoperiodic manipulation critical via melatonin to winter pelage development in mink
Authors: Valtonen M. , Vakkuri O. , Blomstedt L.
Published: September 2010

Quantitive collection of night-time urine every 2nd week was used to elucidate the temporal relationship between changes in melatonin production, weight gain, and priming of the winter pelage under natural light conditions and under a long photoperiod (18L: 6D) imposed at different times in four groups of female mink during autumn. In mink maintained outdoors under the natural photoperiod (group 1), melatonin excretion was 1·80 (s.d. 0·80) ng per night (no. = 3) at the beginning of September, thereafter it began to decrease slowly until November when it was only 0·62 (s.d. 0·32) ng per night. These mink had a normal autumn moult during the first half of October and the winter pelage was mature at the end of November. When mink maintained outdoors were transferred to 18L: 6D on 7 September (group 2), excretion of melatonin decreased promptly, autumn moult was disrupted and winter fur priming delayed. Long photoperiod after mid October (group 3), did not interfere with pelage maturation despite the decrease in melatonin production. Mink implanted with melatonin on 14 July (group 4) showed very high melatonin excretion in early September. In these animals the winter pelage growth was unaffected by the long-day conditions; the pelage matured in mid October. Body weight increased in mink of all groups during autumn. This increase levelled off or a weight loss (group 2) was seen along with the final hair maturation. Activation of hair follicles occurred during a 4 to 6 week period. According to these results, melatonin is the photoperiodic signal to autumnal weight increase and autumn moult but seems not to be necessary for later pelage growth and maturation.
The influence of feeder type and the method of group allocation at weaning on voluntary food intake and growth in piglets
Authors: Pluske J. R. , Williams I. H.
Published: September 2010

Two hundred and ten piglets weaned at 30 days of age and weighing 9 kg were allocated to a 3 × 2 factorial arrangement of treatments to examine the influence of feeder type ('wet and dry' single-space feeder (SSF), 'dry' SSF, or multi-space feeder) and method of group allocation (piglets from different litters were mixed together, or piglets were weaned with their littermates) on performance after weaning. The use of a 'wet and dry' SSF did not enhance growth rate or voluntary food intake (P > 0-05) in the 28 days after weaning. Piglets feeding from 'wet and dry' SSF grew proportionately 0-06 slower in the 28 days following weaning as a result of a 0-45 proportional decrease (P = 0.013) in growth in the 1st week. Piglets mixed together from different litters at weaning grew proportionately 0.34 faster (P = 0.010) in the first 14 days after weaning than piglets weaned as littermates. This initial difference resulted in a 0-14 proportional increase in growth rate from day 1 to 28 (385 v. 339 glday, P < 0.001). Piglets from different litters mixed at weaning consumed proportionately from 0.06 to 0.16 more food (P = 0.096) than piglets weaned as entire litters. No interactions between feeder design and the method of group allocation at weaning occurred for any of the production parameters measured. Single-space feeders failed to increase the production of weaner piglets, whilst mixing non-littermate piglets into a new social group seemingly enhanced performance from weaning to 56 days of age.
Reducing stress in piglets as a means of increasing production after weaning: administration of amperozide or co-mingling of piglets during lactation?
Authors: Pluske J. R. , Williams I. H.
Published: September 2010

The aim of this experiment was to reduce piglet stress and improve performance following moving and mixing at weaning by use of the anti-aggressive drug amperozide and the practice of co-mingling piglets during lactation. Thirty-six piglets weaned at 29 days of age were allocated to a 2 × 2 factorial arrangement of treatments as follows: (1) injection of 1 ml amperozide or placebo (0.9% saline) at the time of weaning, and (2) co-mingling or no comingling of piglets from day 10 of lactation to weaning. At weaning all piglets were injected with pliytohaemagglutinin or saline, their change in skin-fold thickness was measured 24 h later and behaviour was recorded every 5 min for the first 120 min after weaning. At 4 and 24 h after weaning the depth and extent of injuries were recorded. Daily live-weight gain, apparent voluntary food intake and food conversion ratio were not improved (P > 0.05) by administration of amperozide or co-mingling of piglets during lactation. Cell-mediated immunity, as evidenced by a 0.17 proportional decrease (P <; 0.001) in skin-fold thickness following the injection of phytohaemagglutinin, was decreased in piglets not given amperozide at weaning. Injection of amperozide caused a 0.26 proportional increase (P < 0.001) in the amount of time piglets spent lying and caused piglets to display no interest in the feeder (P < 0.001) in the first 2 h after weaning. Consequently piglets not given amperozide were more active, spent more time at the feeder and participated in more aggressive encounters (P < 0.01). A significant interaction occurred between co-mingling and amperozide for mean injury score at both 4 (P < 0.01) and 24 h (P < 0.05) after weaning. When measured 4 h after weaning, co-mingling of piglets during suckling reduced mean injury score regardless of whether piglets were given amperozide or not at weaning. When piglets were not allowed to co-mingle during lactation, amperozide reduced mean injury score proportionately by around 0-44 (P < 0.01) compared with piglets administered with saline. Despite the administration of amperozide and the practice of comingling causing a reduction in stress and an improvement in welfare in this experiment, there was no beneficial effect on performance in the first 2 weeks after weaning.
Marker-assisted selection in an outbred poultry breeding nucleus
Authors: van der Beck S. , van Arendonk J. A. M.
Published: September 2010

The value of using a marker for a quantitative trait locus (QTL) affecting a sex-limited trait in an outbred poultry breeding nucleus was studied. Marker and QTL were in linkage equilibrium in the base population. The recombination rate between marker and QTL was 0-05. A closed nucleus with 9000 chickens per generation was deterministically simulated. The genetic model contained polygenes and a QTL linked to a marker. Genetic effects explained proportionately 0·3 of the phenotypic variance before selection. Under selection, polygenic variance reached an equilibrium and QTL variance decreased continuously over time. Cocks were selected in two steps. First the best cocks of each full-sib family were selected (within-family selection) while final selection took place after information on fiill-sibs was available. Hens were selected after they had completed production. The effect of using marker information in estimating breeding values was studied in an ongoing breeding programme. Transmission of marker alleles was always traceable. Cumulative response over five generations increased proportionately by 0·06 to 0·13 if a marker linked to a QTL that explained 0·2 of the genetic variance was used. Cumulative response increased up to 0·28 if the QTL explained 0-8 of the genetic variance. Additional response due to the use of a marker increased with increasing intensity of within-family selection of cocks, increased with increasing variance explained by the QTL and was higher if within-family selection of cocks was carried out after rather than before their sibs had complete records.
Milk production from grass silage diets: effects of high-protein concentrates for lactating heifers and cows on intake, milk production and milk nitrogen fractions
Authors: Sutton J. D. , Aston K. , Beever D. E. , Dhanoa M. S.
Published: September 2010

To examine the effects of increasing the crude protein (CP) content of concentrates at either equal concentrate intake (increasing CP intake) or at reducing concentrate intake (equal CP intake), 44 multiparous Holstein-Friesian cows were allocated to one of five treatments for weeks 4 to 18 of lactation. The treatments were 6 kg dry matter (DM) per day of concentrates containing nominally 200, 300 or 400 g CP per kg DM or 9 or 3 kg DM per day of concentrates containing 200 or 600 g CP per kg DM respectively. In addition 23 first-calf cows (heifers) were offered 5 kg DM per day of concentrates containing 200, 300 or 400 g CP per kg DM. All the animals were offered first-cut perennial ryegrass silagead libitum. Increasing the concentrate CP content at equal intake caused only small and non-significant increases in silage intake by both heifers and cows. It significantly increased yields of milk and milk protein for both groups but whereas the cows responded to both increments of CP, the heifers only responded to the first. The concentrations of total protein, true protein, casein and non-protein nitrogen (NPN) in milk were significantly increased but the concentration of whey protein remained unchanged. Increasing CP concentration while the concentrate ration was reduced enhanced silage intake but had no significant effect on yields of milk or milk solids. The concentrations of total protein, true protein (non-significant), casein and NPN all increased but whey protein was unchanged. Overall the concentrations of casein and NPN were linearly related to the dietary CP concentration. With both concentrate strategies the proportion of true protein in total milk protein decreased and the proportion of NPN increased with increasing dietary CP concentration but the changes, though highly significant, were relatively small. It is concluded that the strategy of reducing the concentrate ration while maintaining concentrate CP intake causes only small reductions in milk solids production but it is dependent for success on supplies of high-quality grass silage to substitute for the concentrates.
Immunohistochemical quantification of fast-myosin in frozen histological sections of goat limb muscles
Authors: Manabe N. , Azuma Y , Furuya Y. , Kuramitsu K. , Kuribayashi Y. , Nagano N. , Miyamoto H.
Published: September 2010

Fast-myosin in frozen histological sections of eight, 10, 11 and nine muscles of the upper forelimb, lower forelimb, upper hindlimb and lower hindlimb, respectively, of goats was quantified by an immunohistochemical micromethod based on the enzyme-linked immunosorbent assay. The structure of the muscles is well preserved during the immunohistochemical measurement. High fast-myosin levels (more than 201 mg/g total protein) were observed in the triceps brachii (lateral head), rectus femoris, vastus lateralis, semitendinosus, semimembranosus, gastrocnemius (lateral head) and long digital extensor muscles. In contrast, low fast-myosin levels (less than 50 mg/g) were found in the triceps brachii (medial head), superficial digital flexor, vastus intermedialis, and soleus muscles. Fast-myosin-positive fibres (type II or fast-twitch type) were distributed more in the superficial regions than in the deeper regions in the triceps brachii (lateral and long heads), biceps brachii, brachialis, biceps femoris, vastus lateralis, vastus medialis, semimembranosus and gastrocnemius (lateral and medial heads) muscles. In contrast, type IIfibres were distributed more in the deeper regions than in the superficial regions in the extensor carpi radialis, deep digital flexor, cranial tibial, deep digital flexor and superficial digital flexor muscles. When the results obtained by the immunohistochemical micromethod were compared with those obtained by biochemical techniques and by histomorphometrical analyses, high correlations were noted. This technique could be used in research projects to study the muscle characteristics that determine meat quality.
A two-diet system andad libitumlactation feeding of the sow 1. Sow performance
Authors: Neil M. , Ogle B. , Annèr K.
Published: September 2010

Effects of a two-diet system combined withad libitumlactation feeding of sows on food consumption, sow live weight (LW), backfat depth, condition scoring, rebreeding interval, symptoms of agalactia and culling were studied on 60 sows followed for four parities. Feeding regimes were: CR, conventional i.e. restricted during gestation and lactation; SA, a simplified diet offered at a restricted level during gestation and a conventional dietad libitumduring lactation; CA, conventional during gestation and the same dietad libitumduring lactation. During lactation CR sows consumed 5·9 kg food daily (71 MJ metabolizable energy (ME)), whereas SA and CA sows consumed on average 7·0 kg (85 MJ ME), the difference being larger in multiparous than in primiparous sows and larger in the first than in later weeks of lactation. From the second farrowing onward sows on CR treatment were lighter and had thinner backfat and lower condition scores than sows on CA treatment, with sows on SA treatment in between, i.e. approaching the CR sows in the gestation periods and the CA sows in the lactation periods. At first service LW was 130 kg and backfat thickness 14 mm. During the fourth lactation LW averaged 190 kg in CR sows and around 220 kg for SA and CA sows, and backfat thickness was 11 mm in CR sows and around 16 mm in SA and CA sows. SA sows tended to rebreed earlier after weaning than did CR or CA sows. CA sows and in particular SA sows had higher incidences of agalactia symptoms and higher rectal temperatures post partum than CR sows. The culling rates did not differ between feeding regimes, although the predominant causes for culling did, being shoulder lesions and abscesses in thin CR sows and leg disorders in SA and CA sows. At the end of the experiment, however, more SA sows than others were retained in the experimental herd. It was concluded that the SA feeding regime was superior in terms of sow performance, despite an increased incidence of agalactia symptoms.
Partitioning of dietary protein during lactation in the Meishan synthetic and European White breeds of pig
Authors: Sinclair A. G. , Edwards S. A. , Hoste S. , McCartney A. , Fowler V. R.
Published: September 2010

Litter size of commercial European sow lines can be increased by the introduction of genes from the highly prolific, but obese Meishan. The introduction of these genes into sow lines may necessitate different nutritional strategies for lactation. A 2 × 2 × 2 factorial experiment was used to investigate the influence of breed type (Meishan synthetic 50% (M) or purebred European Landrace and Large White (W)) and parity (1 or 3) on response to dietery protein level (H = 178 g crude protein (CP) per kg 8 g lysine per kg, L = 136 g CP per kg 5 g lysine per kg over a 5-week lactation. M were lighter and fatter at farrowing than W (P < 0·001), consumed more food (M = 7·33 v.W = 6·98 (s.e.d. 0·17) kg/day, P < 0·05) but had higher maternal weight loss (M = 16·2 v.W = 11·9 (s.e.d. 1·88) kg, P < 0·05) and higher fat loss (M = 6·5 v. W = 26 (s.e.d. 077) mm P2, P < 0·001) during lactation. Litter growth rate was higher for M than for W (P < 0·005) and for H than for L (P < 0·005). Differences were found between breeds in the partitioning of food protein. Overall, higher correlations between lysine intake and litter growth rates were found in M (r = 0·55, P < 0·001) than in W (r = 0·13, P > 0·05). Lysine intake and sow weight loss were more correlated in W(r = 0·66, P < 0·001) than in M (r = 0·15), P > 0·05). Fat loss was correlated with initial fat depth in M (r = 0·61, P < 0·001) but not in W. Weaning to conception intervals showed a breed × diet interaction (loge days: ML = 1·38, MH = 1·55, WL = 1·73, WH = 1·43, s.e.d. 0·14, P < 0·05). It is concluded that M, with greater initial fat reserves, catabolize more body fat to support greater milk output. This is facilitated by provision of a high protein diet. The W sows do not utilize the extra protein for milk production but instead use it to minimize weight loss (maintain protein status of their own body) which may help prevent an extended weaning to conception interval.
Differences in the hiding behaviour of new-born red deer and hybrid 1/4 Père David's × 3/4 red deer calves
Authors: Endicott-Davies D. R. , Barrie A. N. , Fisher M. W.
Published: September 2010

The behaviour of six pure red deer and six hybrid 3/4 red deer × 1/4 Pere David's deer calves born to pure red deer hinds, was observed from 1 to 24 days of age. Young calves (1 to 8 days of age) spent most time hiding, with neither genotype regularly observed in open view (red deer 11 (s.e. 2·0) % and hybrids 14 (s.e. 2·0) %). However, between 9 and 16 days the hybrid calves were in open view more than twice as often as the red deer calves (38 (s.e. 3·2) %v.18 (s.e. 2·6) %, respectively;P< 0·01). Both genotypes spent over half (both 54 (s.e. 3·5) %) of their time in open view from 17 to 24 days of age. As they grew older, the hybrid calves also spent significantly less time prone and more time standing, compared with the red deer calves.

These results indicate that the behaviour of the hybrids, is somewhat precocious compared with pure red deer calves, and furthermore provide evidence that a component of neonatal behaviour in deer may be inherited.
Modelling lactation curves of dairy cows with emphasis on individual variability
Authors: Pérochon L. , Coulon J. B. , Lescourret F.
Published: September 2010

Five published equations were compared on their ability to adjust different patterns of lactation curves. The equation y(t) = a exp(b1t'2/2 + b2/t – c(l + t'/2)t') with t' = (t – 21·4)/l00 (i) was retained because of the quality of the adjustment and the absence of convergence problems when applied on individual curves. Including an effect of season (SE) and an effect of pregnancy (PE) improved the quality of individual adjustments (no. = 339). The final equation was equal to (i) + SE + PE with SE = a + b cos (2π(wc+ w - l)/52) + c sin(2n(wc+ w - 1)/52), with wc= week of the year at calving, w = week after calving and a = 0·0065, b = -1·26 and c = 0·374, and PE = a (wp–18) e-bwp, with wp= week of pregnancy and a, b fixed parameters. Individual cow characteristics were used to analyse equation (i) parameters. The predictions obtained with this equation and several predictive functions of the equation (i) parameters, which differed in the way they included or not the potential of production, were compared. With no indication of this potential, the prediction was very poor. With the initial production (mean production of the 4th, 5th and 6th days of lactation) as an estimate of this potential, 75% of the lactations had the median of absolute values of errors less than 2·95 kg/day. The results were highly improved by using the yield during the 5th week of lactation. The threshold of 2·95 was reduced to about 2 kg/day. The quality of the individual prediction was better for primiparous than for multiparous cows, and for French Friesian and Montbeliarde cows than for pure or crossbred Holstein cows. Although individual predictions were not always satisfactory, they provided excellent agreement when averaged per group (20 cows).
Effect of increasing digestible undegraded protein supply to dairy cows in late gestation on the yield and composition of milk during the subsequent lactation
Authors: Moorby J. M. , Dewhurst R. J. , Marsden S.
Published: September 2010

Effects of feeding a protein supplement to dairy cows during the dry period on performance during the following lactation were investigated in two experiments. Holstein-Friesian cows were paired towards the end of lactation, and, after drying off, one of each pair received a typical dry cow management regime of ad libitum grass silage (experiment 1), or a mix of grass silage and distillers' grains or pressed beet pulp (experiment 2). The other cows were offered restricted access to the same basal diet, together with ad libitum access to barley straw and 0·5 kg/day high protein maize gluten meal. During the following lactation, animals from both groups were treated without reference to dry period treatment, and were offered equal access to the same lactation diet. Data were analysed by analysis of variance of experiment means and by parallel curve analysis using sample means. In experiment 1, milk yields were similar (27·2 v. 27·9 (s.e.d. 2·12) kg/day for control and supplemented animals respectively) but milk protein yields, and hence concentrations, were significantly higher (P < 0·001) from supplemented animals (28·9 v. 31·8 (s.e.d. 0·58) g/kg). In experiment 2, milk yields were significantly higher (P < 0·001) from supplemented animals (mean 33·3 v. 35·4 (s.e.d. 1·66) kg/day; however, milk protein yields were also significantly increased (P < 0·001) and the change in milk protein concentration was small. No difference in dry-matter intake was recorded in a subset of animals during early lactation in experiment 2. It is hypothesized that the maternal labile body protein pool was maintained or replenished during the dry period by the provision of the protein supplement, and that this had a significant effect on subsequent lactation performance.
Jugular and ovarian venous profiles of progesterone and associated endometrial progesterone concentrations in pregnant and non-pregnant ewes
Authors: Abecia J. A. , Rhind S. M. , Goddard P. J. , McMillen S. R. , Ahmadi S. , Elston D. A.
Published: September 2010

Jugular and ovarian venous profiles of progesterone and endometrial progesterone concentrations were measured in pregnant and non-pregnant ewes. Mean jugular concentrations were not significantly different (3·12v.2·39 μg/l). The incidence of progesterone concentrations above a chosen threshold was significantly greater in the ovarian vein than in the jugular vein (P < 0·05) and was greater (P > 0·05) in the ovarian venous plasma of pregnant than of non-pregnant ewes. Mean endometrial progesterone concentrations were significantly higher in pregnant than in non-pregnant ewes (T < 0·05). It is suggested that the lower progesterone concentrations in the ovarian veins of non-pregnant ewes may have a rôle in pregnancy failure and that ovarian venous concentrations may influence endometrial progesterone concentrations.
Effects of soaking hay fodder for horses on dust and mineral content
Authors: Moore-Colyer M. J. S.
Published: September 2010

Four bales of hay were chosen from a stack of hay, barn-stored for 6 months. Each bale was subdivided into four 2·5 kg sections and one section from each bale was subjected to one of four water soaking treatments: 0, 0·5, 3 or 12 h. Post treatment, the samples were shaken for 9 min under a Negretti LS45F personal dust sampler which sampled the surrounding air. The samples were than analysed for levels of respirable particules < 5 μm in size and for amounts of sodium (Na), potassium (K), phosphorus (P), magnesium (Mg), calcium (Ca) and nitrogen (N). Soaking for 30 min reduced the number of respirable particles by about 90% but also reduced the Na, K and P content of the hay. Soaking for a longer period, at least up to 12 h, did not give a significant further reduction in number of respirable particles but there was loss of Mg and further losses in Na, K, and P. These results clearly indicate that soaking for 30 min is sufficient to reduce the respirable challenge with minimum nutrient loss.
Energetic requirements and physiological adaptation of draught horses to ploughing work
Authors: Pérez R. , Valenzuela S. , Merino V. , Cabezas I. , García M. , Bou R. , Ortiz P.
Published: September 2010

Five Chilean crossbred draught horses were used to study variation in work performance and biochemical and physiological parameters whilst working with a mould board plough (36 kg) for 6 h/day. The draught force, distance travelled, work done and the estimated extra energy for work were determined for each horse. The results showed that the horses used only 68·0 (s.e. 1·9)% of the total available working time ploughing a mean area of 3283 (s.e. 168) m2 with a mean depth and width of furrow of 12·6 and 22·3 cm, respectively. Estimated daily energy expenditure by the horses during work was 2·24 (s.e. 0·02) times maintenance requirements. The small changes observed in the physiological variables: heart rate, respiratory rate, packed cell volume, haemoglobin concentration and enzyme activity of creatine kinase, lactate dehydrogenase and aspartate amino transferase, showed that the horses were exercised under submaximal conditions. However, the significant increase in blood cortisol concentrations showed that horses experienced some degree of stress during ploughing work. The significant increase in blood triglycerides concentration observed after each period of work seems to demonstrate that fat mobilization is an important metabolic pathway as an energy supply for the working muscles during prolonged and low speed draught work.
The effects of wilting of grass prior to ensiling on the response to bacterial inoculation. 2. Intake and performance by dairy cattle over three harvests
Authors: Patterson D. C. , Yan T. , Gordon F. J.
Published: September 2010

Four silages (unwilted with and without inoculant, and wilted with and without inoculant) were prepared from perennial ryegrass swards at each of three harvests over the growing season. The four silages from each of the first (primary growth), second (first regrowth) and third (second regrowth) harvests were offered ad libitum to 48 dairy cows during periods 2, 1 and 3 respectively, in a two (control and additive) × two (unwilted and wilted) × three (harvest (period)) change-over design experiment with 8-week experimental periods. The animals also received a concentrate supplement at 7·0, 6·1 and 5·2 kg dry matter (DM) per day in the first, second and third periods respectively. The supplement was based on barley, molasses and soya-bean meal.

There were significant interactions between inoculation and wilting across the three harvests on silage DM intake (F<0-01), milk yield (P<0·05), and outputs of fat (P<0·01) and protein (P<0·05). Inoculation had no significant effects on silage intake and milk production across the unwilted and wilted silages. However, within the unwilted silages, inoculation significantly increased silage DM intake by 0·46 kg/day (P < 0·05) and fat yield by 0·032 kg/day (P<0·05). In contrast, within the wilted silages inoculation significantly reduced fat yield by 0·030 kg/day (P < 0·05). Wilting of grass prior to ensiling significantly increased silage DM intake by 0·73 kg/day (P <0·001), milk yield by 0·42 kg/day (P<0·05), fat yield by 0·053 kg/day (P < 0·001) and protein yield by 0·047 kg/day (P < 0·001) across the inoculant-treated and untreated silages. The effects however were mainly derived from the untreated silages as within the inoculant-treated materials the differences in silage intake and milk yield were not significant between unwilted and wilted treatments. The results of the current experiment indicate that wilting with no additive significantly improved silage intake and milk production, but otherwise the improvement was reduced with wilting following inoculation. Inoculation significantly increased silage intake and fat yield when used with the unwilted grass, but it significantly reduced fat yield when used with the wilted grass.
Genetic and phenotypic relationships between performance test and reproduction traits in Large White pigs
Authors: Kerr J. C. , Cameron N. D.
Published: September 2010

Genetic and phenotypic relationships between performance test and reproduction traits were estimated, after five generations of divergent selection for components of efficient lean growth, in a population of Large Wltite pigs. On ad-libitum feeding, a total of 4334 pigs were performance tested, of which 884 selected gilts had measurements of reproduction traits. On a restricted feeding regime, 1558 pigs were tested, which included 336 selected gilts with reproduction records. For pigs given food ad libitum, genetic correlations between litter weights at birth and weaning with daily food intake (0·48 and 0·42, s.e. 0·16) and with growth rate on test (0·65 and 0·52) were positive, but correlations with backfat depths were not significantly different from zero. For pigs given food at a restricted level, litter birth weight was positively genetically correlated with growth rate (0·50, s.e. 0·18) and negatively correlated with backfat depths (-0·48, s.e. 0·16). Phenotypic and environmental correlations between performance test and reproduction traits were all less than 0·10 in magnitude, for pigs tested on either feeding regime. The variation in backfat depth enabled detection of a non-linear relationship between predicted breeding values for litter weight at birth with predicted breeding values for average backfat depth of farrowing gilts performance tested on ad-libitum feeding, but not for gilts tested on restricted feeding. The positive genetic correlations between growth rate and daily food intake with litter traits suggested that selection strategies which change growth and daily food intake may result in relatively greater genetic changes in piglet growth rate than in litter size.
The influence of feeding regimen and lactation length on growth, carcass characteristics and meat quality of once-bred gilts
Authors: Ellis M. , Fowler V. R. , Franklin M. F. , Wood J. D. , Varley M. A.
Published: September 2010

A study was made to investigate the influence of pregnancy feeding level (ad libitum (AL) and restricted (R); 2·0 kg food per day) and lactation length on growth, carcass, meat quality and processing characteristics of pregnant gilts (P) slaughtered after weaning on day 0 (P·0), day 10 (P·10) or day 35 (P·35) after farrowing. Non-pregnant (NP) control gilts were slaughtered at live weights of approximately 95 kg (NP-L), 135 kg (NP-M), and 160 kg (NP-H). P gilts were mated at an average age of 172 days and an average live weight of 97 kg. Pregnancy per se reduced live weight and lean growth rates compared with NP-H animals which were slaughtered at a similar age to the once-bred animals. Weight loss during lactation tended to increase with lactation length and was higher for gilts given food ad libitum during pregnancy compared with restricted animals. The weight of closely trimmed lean in the carcass was similar for the P·0, P·10 and P·35 groups (59·5 v. 62·2 v. 58·8 kg respectively; average s.e. 2·42) and all once-bred gilt groups virtually doubled trimmed lean yields during pregnancy and lactation. Curing yields were similar for P and NP gilts. As lactation length increased, the fat content of the longissimus dorsi and of the overlying subcutaneous fat layers tended to decrease and the moisture content of these two tissues increased. The major impact offeeding level was on fat levels which were significantly higher for AL animals. Trimmed lean yields were also higher for AL gilts suggesting that lean growth rates were not being maximized on the R feeding regime. Subjective muscle colour scores indicated that the longissmus dorsi from P gilts was darker than from NP-L animals and muscle colour tended to become darker with increasing lactation length. There were few significant differences between treatment groups for eating quality. Meat from once-bred gilts was generally judged to be tougher than that from NP-L animals and toughness generally increased with lactation length. This study confirms the potential of the once-bred gilt as an efficient producer of pig meat but raises issues regarding meat quality that require further research.
The effect of storage time and number of spermatozoa per insemination dose on semen characteristics and fertilizing capacity of boar semen diluted with Beltsville Thaw Solution (BTS) extender
Authors: Alexopoulos C. , Boscos C. , Saratsis Ph. , Saoulidis C. , Kyriakis S.
Published: September 2010

Semen collection from three boars was performed twice a week, and doses (100 ml each) of semen diluted with Beltsville Thaw Solution (BTS) extender were prepared containing 1 × 109, 3 × 109 and 5 × 109 spermatozoa. Diluted semen was then stored at 17°C for a maximum of 120 h. Percentage of motile spermatozoa (PMS) and type of spermatozoa motility (TSM) were determined by microscopic examinations performed at 24-h intervals, from zero time (beginning of storage at 17°C) up to 120 h of storage, and a sperm motility index (SMI) calculated. Sows (no. = 360) were divided into nine experimental groups (40 sows per group) and a combination of number of spermatozoa per dose and storage time was used for artificial insemination in each group. All the animals were inseminated twice at 12 and 36 h after detection of standing oestrus by a teaser boar. Return and farrowing data including litter size were recorded. PMS, TSM and SMI decreased significantly (P < 0·01) from 48 h up to 120 h of storage, this being more marked in semen doses of 5 × 109 spermatozoa. When semen stored for up to 24 h was used, no significant differences (P > 0·05) regarding return rates and farrowing rates were observed among the three groups inseminated with semen containing different numbers of spermatozoa per dose. Inseminations with semen doses of 1 × 109 and 3 × 109 spermatozoa stored for 48 to 72 h have significantly lower return rates (P < 0·05) and significantly higher farrowing rates (P < 0·05) than with doses of 5 × 109 spermatozoa. Inseminations with semen stored for more than 72 h gave relatively higher return rates and lower farrowing rates. Semen storage time and number of spermatozoa per dose appeared to reduce litter size only where semen doses of 5 X109 spermatozoa were inseminated after storage for more than 48 h.