bluuebunny/crossref_metadata_2025 huggingface dataset:
Desert Ascetics of Egypt
Authors: Brooks Hedstrom Darlene L.
Published: February 2024

Egypt is revered as the home of the famous Desert Ascetics, who first embraced a monastic life and established homosocial communities on the borders of their urban centres in the Nile Valley. Regarded as angels and warriors, the wisdom of the Desert Ascetics formed part of the oral and literary tradition of wonder-working saints whose commitment to asceticism was legendary and inspirational. This book grounds the mythologized stories of Desert Ascetics in the materiality of the desert, demonstrating the closeness of the desert, the connections between non-monastic and monastic communities, and the exciting insights into lived monasticism through the archaeology of monasticism in Egypt.
Hong Kong Pop Culture in the 1980s
Authors: Chu Yiu-Wai
Published: February 2024

This book deals with the 1980s - the 'golden decade' of Hong Kong pop culture - in which a cosmopolitan lifestyle of pop and chic emerged in the city. Bookended by two major historical incidents, the 1980s will probably enter the annals of Hong Kong history as the decade that defined its future after reversion to Mainland China. Having witnessed and experienced the rise of Hong Kong pop culture to unprecedented heights in this decade, the author enhances its context through a story about his own personal belongings. Examining popular genres including television, film, music, fashion, disco and city magazine, this book teases out the distinctive aspects of Hong Kong pop culture that defined (his) Hong Kong. As Hong Kong has been undergoing drastic changes in recent years, it is necessary to point toward new imaginaries by re-examining its development. Toward this end, this book will shed light on an important research area of Hong Kong Studies as an academic discipline.
Gender and Self-Fashioning at the Intersection of Art and Science
Authors: Powell-Warren Catherine
Published: February 2024

At once collector, botanist, reader, artist, and patron, Agnes Block is best described as a cultural producer. A member of an influential network in her lifetime, today she remains a largely obscure figure. The socioeconomic and political barriers faced by early modern women, together with a male-dominated tradition in art history, have meant that too few stories of women's roles in the creation, production, and consumption of art have reached us. This book seeks to write Block and her contributions into the art and cultural history of the seventeenth-century Netherlands, highlighting the need for and advantages of a multifaceted approach to research on early modern women. Examining Block's achievements, relationships, and objects reveals a woman who was independent, knowledgeable, self-aware, and not above self-promotion. Though her gender brought few opportunities and many barriers, Agnes Block succeeded in fashioning herself as Flora Batava, a liefhebber at the intersection of art and science.
The Paradoxes of Japan's Cultural Identity
Authors: Starrs Roy
Published: February 2024

Japan is widely regarded as having a unique culture and a strong national identity. Paradoxically, however, many basic elements of Japanese culture are not originally Japanese. Since the beginning of its history, Japan has been one of the world's major importers of foreign cultures. Its culture was thoroughly 'hybrid' long before that word became fashionable in contemporary global studies. But this does not mean that Japan's culture lacks originality. The Japanese have always made strikingly original contributions, even improvements, to whatever they imported. Even more significantly, the 'hybridity' of their culture produced ongoing tensions that served as a kind of creative dynamo for Japanese writers, artists, and intellectuals. This book explores the fundamental creative tension between the native and the foreign in many areas of Japanese culture, from politics and religion to art and literature - a tension also often interpreted as between tradition and modernity.
A Systems-Thinking Model of Data Management and Use in US Archaeology
Authors: Bollwerk Elizabeth , Gupta Neha , Smith Jolene
Published: February 2024

Overview

Archaeology in the United States is caught in a “curation crisis” (Childs 1995; Childs and Warner 2019; Marquardt et al. 1982; SAA Advisory Committee on Curation 2003; Trimble and Marino 2003) and a “digital data crisis” (or “deluge”) more specifically (Bevan 2015; Clarke 2015; Kansa and Kansa 2021; Katsianis et al. 2022; Kersel 2015; McManamon et al. 2017:239–240; Rivers Cofield et al. 2024). Recent estimates suggest that, collectively, over 1.4 billion dollars are spent annually to support archaeological work that is mandated by federal law (SRI Foundation 2020). Although substantial efforts are underway to generate and provide mechanisms for managing, curating, and sharing the resultant digital data, we suggest that a critical step that has yet to be taken is to describe and visualize the components, connections, and causal dynamics of the US digital data system as it currently functions. Here, we specifically apply a “systems thinking” approach to produce such a high-level model of this system. We argue that understanding and visualizing this system will help us all “think bigger” (Heilen and Manney 2023); identify sources of knowledge, opportunities for critical analysis, collaboration, and capacity building; and increase much-needed archaeological digital literacy (Kansa and Kansa 2022). We conceptualize this as bringing “equilibrium” to the system, and in this article, we make several suggestions on how to bring this about. These insights can enable practitioners to better understand their roles in and contributions to the overall system and to evaluate efforts to improve data sharing, management, and curation practices not only within their organizations and departments but beyond.
A content analysis: analyzing topics of conversation under the #sustainability hashtag on Twitter
Authors: Gerber Aydan
Published: February 2024

This study aimed to identify and understand the major topics of discussion under the #sustainability hashtag on Twitter (now known as “X”) and understand user engagement. The sharp increase in social media usage combined with a rise in climate anomalies in recent years makes the area of sustainability with respect to social media a critical topic. Python was used to gather Twitter posts between January 1, 2023, and March 1, 2023. User engagement metrics were analyzed using a variety of statistical analysis methods, including keyword-frequency analysis and Latent Dirichlet Allocation (LDA), which were used to identify significant topics of discussion under the #sustainability hashtag. Additionally, histograms and scatter plots were used to visualize user engagement. LDA analysis was conducted with 7 topics after trials were run with various topics and results were analyzed to determine which number of topics best fit the dataset. The frequency analysis provided a basic overview of the discourse surrounding #sustainability with the topics of technology, business and industry, environmental awareness, and discussion of the future. The LDA model provided a more comprehensive view, including additional topics such as Environmental, Social, and Governance (ESG) and infrastructure, investing, collaboration, and education. These findings have implications for researchers, businesses, organizations, and politicians seeking to align their strategies and actions with the major topics surrounding sustainability on Twitter to have a greater impact on their audience. Researchers can use the results of this study to guide further research on the topic or contextualize their study with existing literature within the field of sustainability.
Measures of maximal entropy of bounded density shifts
Authors: GARCÍA-RAMOS FELIPE , PAVLOV RONNIE , REYES CARLOS
Published: February 2024

We find sufficient conditions for bounded density shifts to have a unique measure of maximal entropy. We also prove that every measure of maximal entropy of a bounded density shift is fully supported. As a consequence of this, we obtain that bounded density shifts are surjunctive.
The Liar’s Dividend: Can Politicians Claim Misinformation to Evade Accountability?
Authors: SCHIFF KAYLYN JACKSON , SCHIFF DANIEL S. , BUENO NATÁLIA S.
Published: February 2024

This study addresses the phenomenon of misinformation about misinformation, or politicians “crying wolf” over fake news. Strategic and false claims that stories are fake news or deepfakes may benefit politicians by helping them maintain support after a scandal. We posit that this benefit, known as the “liar’s dividend,” may be achieved through two politician strategies: by invoking informational uncertainty or by encouraging oppositional rallying of core supporters. We administer five survey experiments to over 15,000 American adults detailing hypothetical politician responses to stories describing real politician scandals. We find that claims of misinformation representing both strategies raise politician support across partisan subgroups. These strategies are effective against text-based reports of scandals, but are largely ineffective against video evidence and do not reduce general trust in media. Finally, these false claims produce greater dividends for politicians than alternative responses to scandal, such as remaining silent or apologizing.
Characteristics and Trends of the Campbell Plateau Meander in the Southern Ocean: 1993–2020
Authors: Liu Xinlong , Meyer Amelie , Chapman Christopher C.
Published: February 2024

Meanders are significant features of the Antarctic Circumpolar Current in the Southern Ocean and sites of enhanced upwelling, cross‐frontal tracer fluxes, and exchanges between the surface and deep ocean. They often overlap the locations of fronts and are linked to topographic features. While much is known about Southern Ocean fronts and how they are changing, the response of meanders to climate change is largely unexplored. In this study, we investigate the Campbell Plateau meander south of New Zealand. We apply a local gradient maxima method to satellite altimetry data to identify the position of the meander and estimate its width, geostrophic current speed, and associated trends from 1993 to 2020. We find that the position of the meander has been relatively fixed, except for a section downstream of the Plateau, which has shifted northward by about 0.4° latitude per decade. The meander has become flatter at the western edge of the Plateau, but steeper at the eastern edge of the Plateau. Overall, the meander has widened by 2 km per decade and accelerated by 0.01 m s−1 per decade, particularly downstream of the Plateau. These findings are consistent with other work on standing meanders in the Southern Ocean. Increases in eddy kinetic energy and of the South Pacific Gyre index support our hypotheses that changes in the downstream jet stability and the South Pacific Gyre contribute to these observed trends. The impacts of these trends on cross‐frontal transport remain to be evaluated.
Understanding Biases in Indian Ocean Seasonal SST in CMIP6 Models
Authors: McKenna Sebastian , Santoso Agus , Sen Gupta Alex , Taschetto Andréa S.
Published: February 2024

The latest generation of climate models continue to exhibit biases in their representation of climatological sea surface temperature (SST), affecting their ability to simulate climate variability including the Indian Ocean Dipole which is typically too strong. Here, we analyze the surface layer heat budget of the Indian Ocean to diagnose the processes leading to biases in climatological SST biases in 20 Coupled Model Intercomparison Project Phase 6 (CMIP6) models, in comparison to a suite of observational and reanalysis products. In the western tropical Indian Ocean, we find that weaker than observed winds reduce the strength of surface currents leading to warm SST bias. In the south‐eastern tropical Indian Ocean, overly strong southeasterly winds are associated with overestimated coastal upwelling that increases cooling across CMIP6 models. In the Arabian Sea, overly strong surface winds increase latent heat loss and leads to cool SST biases. We also analyze other regions like the Bay of Bengal, where a persistent cool bias cannot be explained by seasonal heat budgets, and southern Indian Ocean, where overly strong surface winds are responsible for cool SST biases. These biases in surface processes are supported by intermodel relationships between relevant variables, thus explaining differences in simulating the climatological SSTs across the model ensemble. Our analysis suggests that biases in atmospheric processes in particular surface winds are a primary cause of biases in Indian Ocean SST. Reducing these biases would improve the simulation of climate variability in the Indian Ocean toward more reliable climate projections and predictions.
Hsa_circ_0007990 promotes breast cancer growth via inhibiting YBX1 protein degradation to activate E2F1 transcription
Authors: Xu Tao , Xiong Mengqiu , Hong Qiwei , Pan Bei , Xu Mu , Wang Ying , Sun Yalan , Sun Huiling , Pan Yuqin , Wang Shukui , He Bangshun
Published: February 2024

Breast cancer (BC) is the most commonly diagnosed malignant tumour in females worldwide. Although remarkable advances in early detection and treatment strategies have led to decreased mortality, recurrence and metastasis remain the major causes of cancer death in BC patients. Increasing evidence has demonstrated that circular RNAs (circRNAs) play critical roles in cancer progression. However, the detailed biological functions and molecular mechanisms of circRNAs in BC are unclear. The aim of this study was to investigate the possible role of circRNAs in the progression of BC. Differentially expressed circRNAs in BC were identified by integrating breast tumour-associated somatic CNV data and circRNA high-throughput sequencing. Aberrant hsa_circ_0007990 expression and host gene copy number were detected in BC cell lines via quantitative polymerase chain reaction (qPCR). The expression level of hsa_circ_0007990 in BC tissues was validated by in situ hybridization (ISH). Loss- and gain-of-function experiments were performed in vitro and in vivo, respectively, to explore the potential biological function of hsa_circ_0007990 in BC. The underlying mechanisms of hsa_circ_0007990 were investigated through MS2 RNA pull-down, RNA immunoprecipitation, RNA fluorescence in situ hybridization, immunofluorescence, chromatin immunoprecipitation and luciferase reporter assays. The levels of hsa_circ_0007990 were elevated in BC tissues and cell lines, an effect that was partly due to host gene copy number gains. Functional assays showed that hsa_circ_0007990 promoted BC cell growth. Mechanistically, hsa_circ_0007990 could bind to YBX1 and inhibit its degradation by preventing ubiquitin/proteasome-dependent degradation, thus enhancing the expression of the cell cycle-associated gene E2F1. Rescue experiments suggested that hsa_circ_0007990 promoted BC progression through YBX1. In general, our study demonstrated that hsa_circ_0007990 modulates the ubiquitination and degradation of YBX1 protein and further regulates E2F1 expression to promote BC progression. We explored the possible function and molecular mechanism of hsa_circ_0007990 in BC and identified a novel candidate target for the treatment of BC.
HMGB1 promotes mitochondrial transfer between hepatocellular carcinoma cells through RHOT1 and RAC1 under hypoxia
Authors: Jing Mengjia , Xiong Xiaofeng , Mao Xin , Song Qianben , Zhang Lumiao , Ouyang Yiming , Pang Yingzhi , Fu Yu , Yan Wei
Published: February 2024

Mitochondrial transfer plays an important role in various diseases, and many mitochondrial biological functions can be regulated by HMGB1. To explore the role of mitochondrial transfer in hepatocellular carcinoma (HCC) and its relationship with HMGB1, field emission scanning electron microscopy, immunofluorescence, and flow cytometry were used to detect the mitochondrial transfer between HCC cells. We found that mitochondrial transfer between HCC cells was confirmed using tunnel nanotubes (TNTs). The transfer of mitochondria from the highly invasive HCC cells to the less invasive HCC cells could enhance the migration and invasion ability of the latter. The hypoxic conditions increased the mitochondrial transfer between HCC cells. Then the mechanism was identified using co-immunoprecipitation, luciferase reporter assay, and chromatin immunoprecipitation. We found that RHOT1, a mitochondrial transport protein, promoted mitochondrial transfer and the migration and metastasis of HCC cells during this process. Under hypoxia, HMGB1 further regulated RHOT1 expression by increasing the expression of NFYA and NFYC subunits of the NF-Y complex. RAC1, a protein associated with TNTs formation, promoted mitochondrial transfer and HCC development. Besides, HMGB1 regulated RAC1 aggregation to the cell membrane under hypoxia. Finally, the changes and significance of related molecules in clinical samples of HCC were analyzed using bioinformatics and tissue microarray analyses. We found that HCC patients with high HMGB1, RHOT1, or RAC1 expression exhibited a relatively shorter overall survival period. In conclusion, under hypoxic conditions, HMGB1 promoted mitochondrial transfer and migration and invasion of HCC cells by increasing the expression of mitochondrial transport protein RHOT1 and TNTs formation-related protein RAC1.
Psychological well-being in Europe after the outbreak of war in Ukraine
Authors: Scharbert Julian , Humberg Sarah , Kroencke Lara , Reiter Thomas , Sakel Sophia , ter Horst Julian , Utesch Katharina , Gosling Samuel D. , Harari Gabriella , Matz Sandra C. , Schoedel Ramona , Stachl Clemens , Aguilar Natalia M. A. , Amante Dayana , Aquino Sibele D. , Bastias Franco , Bornamanesh Alireza , Bracegirdle Chloe , Campos Luís A. M. , Chauvin Bruno , Coetzee Nicoleen , Dorfman Anna , dos Santos Monika , El-Haddad Rita W. , Fajkowska Malgorzata , Göncü-Köse Asli , Gnisci Augusto , Hadjisolomou Stavros , Hale William W. , Katzir Maayan , Khechuashvili Lili , Kirchner-Häusler Alexander , Kotzur Patrick F. , Kritzler Sarah , Lu Jackson G. , Machado Gustavo D. S. , Martskvishvili Khatuna , Mottola Francesca , Obschonka Martin , Paolini Stefania , Perugini Marco , Rohmer Odile , Saeedian Yasser , Sergi Ida , Shani Maor , Skimina Ewa , Smillie Luke D. , Talaifar Sanaz , Talhelm Thomas , Tokat Tülüce , Torres Ana , Torres Claudio V. , Van Assche Jasper , Wei Liuqing , Yalçın Aslı , van Zalk Maarten , Bühner Markus , Back Mitja D.
Published: February 2024

The Russian invasion of Ukraine on February 24, 2022, has had devastating effects on the Ukrainian population and the global economy, environment, and political order. However, little is known about the psychological states surrounding the outbreak of war, particularly the mental well-being of individuals outside Ukraine. Here, we present a longitudinal experience-sampling study of a convenience sample from 17 European countries (total participants = 1,341, total assessments = 44,894, countries with >100 participants = 5) that allows us to track well-being levels across countries during the weeks surrounding the outbreak of war. Our data show a significant decline in well-being on the day of the Russian invasion. Recovery over the following weeks was associated with an individual’s personality but was not statistically significantly associated with their age, gender, subjective social status, and political orientation. In general, well-being was lower on days when the war was more salient on social media. Our results demonstrate the need to consider the psychological implications of the Russo-Ukrainian war next to its humanitarian, economic, and ecological consequences.
Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane H+-ATPase
Authors: Fuji Saashia , Yamauchi Shota , Sugiyama Naoyuki , Kohchi Takayuki , Nishihama Ryuichi , Shimazaki Ken-ichiro , Takemiya Atsushi
Published: February 2024

Plasma membrane H+-ATPase provides the driving force for light-induced stomatal opening. However, the mechanisms underlying the regulation of its activity remain unclear. Here, we show that the phosphorylation of two Thr residues in the C-terminal autoinhibitory domain is crucial for H+-ATPase activation and stomatal opening in Arabidopsis thaliana. Using phosphoproteome analysis, we show that blue light induces the phosphorylation of Thr-881 within the C-terminal region I, in addition to penultimate Thr-948 in AUTOINHIBITED H+-ATPASE 1 (AHA1). Based on site-directed mutagenesis experiments, phosphorylation of both Thr residues is essential for H+ pumping and stomatal opening in response to blue light. Thr-948 phosphorylation is a prerequisite for Thr-881 phosphorylation by blue light. Additionally, red light-driven guard cell photosynthesis induces Thr-881 phosphorylation, possibly contributing to red light-dependent stomatal opening. Our findings provide mechanistic insights into H+-ATPase activation that exploits the ion transport across the plasma membrane and light signalling network in guard cells.
A neural signature for the subjective experience of threat anticipation under uncertainty
Authors: Liu Xiqin , Jiao Guojuan , Zhou Feng , Kendrick Keith M. , Yao Dezhong , Gong Qiyong , Xiang Shitong , Jia Tianye , Zhang Xiao-Yong , Zhang Jie , Feng Jianfeng , Becker Benjamin
Published: February 2024

Uncertainty about potential future threats and the associated anxious anticipation represents a key feature of anxiety. However, the neural systems that underlie the subjective experience of threat anticipation under uncertainty remain unclear. Combining an uncertainty-variation threat anticipation paradigm that allows precise modulation of the level of momentary anxious arousal during functional magnetic resonance imaging (fMRI) with multivariate predictive modeling, we train a brain model that accurately predicts subjective anxious arousal intensity during anticipation and test it across 9 samples (total n = 572, both gender). Using publicly available datasets, we demonstrate that the whole-brain signature specifically predicts anxious anticipation and is not sensitive in predicting pain, general anticipation or unspecific emotional and autonomic arousal. The signature is also functionally and spatially distinguishable from representations of subjective fear or negative affect. We develop a sensitive, generalizable, and specific neuroimaging marker for the subjective experience of uncertain threat anticipation that can facilitate model development.
Chronic endoplasmic reticulum stress in myotonic dystrophy type 2 promotes autoimmunity via mitochondrial DNA release
Authors: Rösing Sarah , Ullrich Fabian , Meisterfeld Susann , Schmidt Franziska , Mlitzko Laura , Croon Marijana , Nattrass Ryan G , Eberl Nadia , Mahlberg Julia , Schlee Martin , Wieland Anja , Simon Philipp , Hilbig Daniel , Reuner Ulrike , Rapp Alexander , Bremser Julia , Mirtschink Peter , Drukewitz Stephan , Zillinger Thomas , Beissert Stefan , Paeschke Katrin , Hartmann Gunther , Trifunovic Aleksandra , Bartok Eva , Günther Claudia
Published: February 2024

Myotonic dystrophy type 2 (DM2) is a tetranucleotide CCTG repeat expansion disease associated with an increased prevalence of autoimmunity. Here, we identified an elevated type I interferon (IFN) signature in peripheral blood mononuclear cells and primary fibroblasts of DM2 patients as a trigger of chronic immune stimulation. Although RNA-repeat accumulation was prevalent in the cytosol of DM2-patient fibroblasts, type-I IFN release did not depend on innate RNA immune sensors but rather the DNA sensor cGAS and the prevalence of mitochondrial DNA (mtDNA) in the cytoplasm. Sublethal mtDNA release was promoted by a chronic activation of the ATF6 branch of the unfolded protein response (UPR) in reaction to RNA-repeat accumulation and non-AUG translated tetrapeptide expansion proteins. ATF6-dependent mtDNA release and resulting cGAS/STING activation could also be recapitulated in human THP-1 monocytes exposed to chronic endoplasmic reticulum (ER) stress. Altogether, our study demonstrates a novel mechanism by which large repeat expansions cause chronic endoplasmic reticulum stress and associated mtDNA leakage. This mtDNA is, in turn, sensed by the cGAS/STING pathway and induces a type-I IFN response predisposing to autoimmunity. Elucidating this pathway reveals new potential therapeutic targets for autoimmune disorders associated with repeat expansion diseases.
Inorganic perovskite-based active multifunctional integrated photonic devices
Authors: Han Qi , Wang Jun , Tian Shuangshuang , Hu Shen , Wu Xuefeng , Bai Rongxu , Zhao Haibin , Zhang David W. , Sun Qingqing , Ji Li
Published: February 2024

The development of highly efficient active integrated photonic circuits is crucial for advancing information and computing science. Lead halide perovskite semiconductors, with their exceptional optoelectronic properties, offer a promising platform for such devices. In this study, active micro multifunctional photonic devices were fabricated on monocrystalline CsPbBr3 perovskite thin films using a top-down etching technique with focused ion beams. The etched microwire exhibited a high-quality micro laser that could serve as a light source for integrated devices, facilitating angle-dependent effective propagation between coupled perovskite-microwire waveguides. Employing this strategy, multiple perovskite-based active integrated photonic devices were realized for the first time. These devices included a micro beam splitter that coherently separated lasing signals, an X-coupler performing transfer matrix functions with two distinguishable light sources, and a Mach-Zehnder interferometer manipulating the splitting and coalescence of coherent light beams. These results provide a proof-of-concept for active integrated functionalized photonic devices based on perovskite semiconductors, representing a promising avenue for practical applications in integrated optical chips.
Atlantic Water warming increases melt below Northeast Greenland’s last floating ice tongue
Authors: Wekerle Claudia , McPherson Rebecca , von Appen Wilken-Jon , Wang Qiang , Timmermann Ralph , Scholz Patrick , Danilov Sergey , Shu Qi , Kanzow Torsten
Published: February 2024

The 79 North Glacier (79NG) features Greenland’s largest floating ice tongue. Even though its extent has not changed significantly in recent years, observations have indicated a major thinning of the ice tongue from below. Both ocean warming and an increase in subglacial discharge from the ice sheet induced by atmospheric warming could increase the basal melt; however, available observations alone cannot tell which of these is the main driver. Here, we employ a global simulation which explicitly resolves the ocean circulation in the cavity with 700 m resolution to disentangle the impact of the ocean and atmosphere. We find that the interannual variability of basal melt below 79NG over the past 50 years is mainly associated with changes in the temperature of the Atlantic Intermediate Water inflow, which can be traced back across the Northeast Greenland continental shelf to the eastern Fram Strait with a lag of 3 years.
Cepharanthine analogs mining and genomes of Stephania accelerate anti-coronavirus drug discovery
Authors: Leng Liang , Xu Zhichao , Hong Bixia , Zhao Binbin , Tian Ya , Wang Can , Yang Lulu , Zou Zhongmei , Li Lingyu , Liu Ke , Peng Wanjun , Liu Jiangning , An Zhoujie , Wang Yalin , Duan Baozhong , Hu Zhigang , Zheng Chuan , Zhang Sanyin , Li Xiaodong , Li Maochen , Liu Zhaoyu , Bi Zenghao , He Tianxing , Liu Baimei , Fan Huahao , Song Chi , Tong Yigang , Chen Shilin
Published: February 2024

Cepharanthine is a secondary metabolite isolated from Stephania. It has been reported that it has anti-conronaviruses activities including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Here, we assemble three Stephania genomes (S. japonica, S. yunnanensis, and S. cepharantha), propose the cepharanthine biosynthetic pathway, and assess the antiviral potential of compounds involved in the pathway. Among the three genomes, S. japonica has a near telomere-to-telomere assembly with one remaining gap, and S. cepharantha and S. yunnanensis have chromosome-level assemblies. Following by biosynthetic gene mining and metabolomics analysis, we identify seven cepharanthine analogs that have broad-spectrum anti-coronavirus activities, including SARS-CoV-2, Guangxi pangolin-CoV (GX_P2V), swine acute diarrhoea syndrome coronavirus (SADS-CoV), and porcine epidemic diarrhea virus (PEDV). We also show that two other genera, Nelumbo and Thalictrum, can produce cepharanthine analogs, and thus have the potential for antiviral compound discovery. Results generated from this study could accelerate broad-spectrum anti-coronavirus drug discovery.
Exploiting lung adaptation and phage steering to clear pan-resistant Pseudomonas aeruginosa infections in vivo
Authors: Ashworth Eleri A. , Wright Rosanna C. T. , Shears Rebecca K. , Wong Janet K. L. , Hassan Akram , Hall James P. J. , Kadioglu Aras , Fothergill Joanne L.
Published: February 2024

Pseudomonas aeruginosa is a major nosocomial pathogen that causes severe disease including sepsis. Carbapenem-resistant P. aeruginosa is recognised by the World Health Organisation as a priority 1 pathogen, with urgent need for new therapeutics. As such, there is renewed interest in using bacteriophages as a therapeutic. However, the dynamics of treating pan-resistant P. aeruginosa with phage in vivo are poorly understood. Using a pan-resistant P. aeruginosa in vivo infection model, phage therapy displays strong therapeutic potential, clearing infection from the blood, kidneys, and spleen. Remaining bacteria in the lungs and liver displays phage resistance due to limiting phage adsorption. Yet, resistance to phage results in re-sensitisation to a wide range of antibiotics. In this work, we use phage steering in vivo, pre-exposing a pan resistant P. aeruginosa infection with a phage cocktail to re-sensitise bacteria to antibiotics, clearing the infection from all organs.
Harmonic amide bond density as a game-changer for deciphering the crosslinking puzzle of polyamide
Authors: Xue Yu-Ren , Liu Chang , Ma Zhao-Yu , Zhu Cheng-Ye , Wu Jian , Liang Hong-Qing , Yang Hao-Cheng , Zhang Chao , Xu Zhi-Kang
Published: February 2024

It is particularly essential to analyze the complex crosslinked networks within polyamide membranes and their correlation with separation efficiency for the insightful tailoring of desalination membranes. However, using the degree of network crosslinking as a descriptor yields abnormal analytical outcomes and limited correlation with desalination performance due to imperfections in segmentation and calculation methods. Herein, we introduce a more rational parameter, denoted as harmonic amide bond density (HABD), to unravel the relationship between the crosslinked networks of polyamide membranes and their desalination performance. HABD quantifies the number of distinct amide bonds per unit mass of polyamide, based on a comprehensive segmentation of polyamide structure and consistent computational protocols derived from X-ray photoelectron spectroscopy data. Compared to its counterpart, HABD overcomes the limitations and offers a more accurate depiction of the crosslinked networks. Empirical data validate that HABD exhibits the expected correlation with the salt rejection and water permeance of reverse osmosis and nanofiltration polyamide membranes. Notably, HABD is applicable for analyzing complex crosslinked polyamide networks formed by highly functional monomers. By offering a powerful toolbox for systematic analysis of crosslinked polyamide networks, HABD facilitates the development of permselective membranes with enhanced performance in desalination applications.
Clinical and biomarker results from a phase II trial of combined cabozantinib and durvalumab in patients with chemotherapy-refractory colorectal cancer (CRC): CAMILLA CRC cohort
Authors: Saeed Anwaar , Park Robin , Pathak Harsh , Al-Bzour Ayah Nedal , Dai Junqiang , Phadnis Milind , Al-Rajabi Raed , Kasi Anup , Baranda Joaquina , Sun Weijing , Williamson Stephen , Chiu Yu-Chiao , Osmanbeyoglu Hatice Ulku , Madan Rashna , Abushukair Hassan , Mulvaney Kelly , Godwin Andrew K. , Saeed Azhar
Published: February 2024

CAMILLA is a basket trial (NCT03539822) evaluating cabozantinib plus the ICI durvalumab in chemorefractory gastrointestinal cancer. Herein, are the phase II colorectal cohort results. 29 patients were evaluable. 100% had confirmed pMMR/MSS tumors. Primary endpoint was met with ORR of 27.6% (95% CI 12.7-47.2%). Secondary endpoints of 4-month PFS rate was 44.83% (95% CI 26.5-64.3%); and median OS was 9.1 months (95% CI 5.8-20.2). Grade≥3 TRAE occurred in 39%. In post-hoc analysis of patients with RAS wild type tumors, ORR was 50% and median PFS and OS were 6.3 and 21.5 months respectively. Exploratory spatial transcriptomic profiling of pretreatment tumors showed upregulation of VEGF and MET signaling, increased extracellular matrix activity and preexisting anti-tumor immune responses coexisting with immune suppressive features like T cell migration barriers in responders versus non-responders. Cabozantinib plus durvalumab demonstrated anti-tumor activity, manageable toxicity, and have led to the activation of the phase III STELLAR-303 trial.
Diffractive optical computing in free space
Authors: Hu Jingtian , Mengu Deniz , Tzarouchis Dimitrios C. , Edwards Brian , Engheta Nader , Ozcan Aydogan
Published: February 2024

Structured optical materials create new computing paradigms using photons, with transformative impact on various fields, including machine learning, computer vision, imaging, telecommunications, and sensing. This Perspective sheds light on the potential of free-space optical systems based on engineered surfaces for advancing optical computing. Manipulating light in unprecedented ways, emerging structured surfaces enable all-optical implementation of various mathematical functions and machine learning tasks. Diffractive networks, in particular, bring deep-learning principles into the design and operation of free-space optical systems to create new functionalities. Metasurfaces consisting of deeply subwavelength units are achieving exotic optical responses that provide independent control over different properties of light and can bring major advances in computational throughput and data-transfer bandwidth of free-space optical processors. Unlike integrated photonics-based optoelectronic systems that demand preprocessed inputs, free-space optical processors have direct access to all the optical degrees of freedom that carry information about an input scene/object without needing digital recovery or preprocessing of information. To realize the full potential of free-space optical computing architectures, diffractive surfaces and metasurfaces need to advance symbiotically and co-evolve in their designs, 3D fabrication/integration, cascadability, and computing accuracy to serve the needs of next-generation machine vision, computational imaging, mathematical computing, and telecommunication technologies.
Offshoring emissions through used vehicle exports
Authors: Newman Saul Justin , Schulte Kayla , Morellini Micol Matilde , Rahal Charles , Leasure Douglas R.
Published: February 2024

Policies to reduce transport emissions often overlook the international flow of used vehicles. We quantify the rate at which used vehicles generated CO2 and pollution for all used vehicles exported from Great Britain—a globally leading used vehicle exporter—across 2005–2021. Destined for low–middle-income countries, exported vehicles fail roadworthiness standards and, even under extremely optimistic ‘functioning-as-new’ assumptions, generate at least 13–53% more emissions than scrapped or on-road vehicles.
Impact of gamma irradiation on physico-chemical and electromagnetic interference shielding properties of Cu2O nanoparticles reinforced LDPE nanocomposite films
Authors: Bekhit Mohamad , Fathy E. S. , Sharaf A. , Shiple M.
Published: February 2024

In the current work, cuprous oxide (Cu2O) nanoparticles coated with Tween 80 were successfully synthesized via the chemical reduction method. Nanocomposites composed of low-density polyethylene (LDPE) and different ratios of Cu2O nanoparticles were fabricated by the melt mixing process. 10% of ethyl vinyl acetate (EVA) as a compatibilizing agent was added to the molten LDPE matrix and the mixing process continued until homogenous nanocomposites were fabricated. To study the influence of ionizing radiation on the fabricated samples, the prepared species were exposed to 50 and 100 kGy of gamma rays. The synthesized Cu2O nanoparticles were investigated by transmission electron microscopy (TEM) and X-ray diffraction (XRD). XRD and TEM analysis illustrated the successful formation of spherical Cu2O nanoparticles with an average size of 16.8 nm. The as-prepared LDPE/Cu2O nanocomposites were characterized via different techniques such as mechanical, thermal, morphological, XRD, and FTIR. Electromagnetic interference shielding (EMI) of the different nanocomposite formulations was performed as a promising application for these materials in practical life. The electromagnetic shielding effectiveness (SE) of the produced samples was measured in the X-band of the radio frequency range from 8 to 12 GHz using the vector network analyzer (VNA) and a proper waveguide. All the samples were studied before and after gamma-ray irradiation under the same conditions of pressure and temperature. The shielding effectiveness increased significantly from 25 dB for unirradiated samples to 35 dB with samples irradiated with 100 kGy, which reflects 40% enhancement in the effectiveness of the shielding.
Effects of sepiolite addition to acrylic-latex paint on pull-off adhesion strength in nanosilver-impregnated and thermally-modified beech (Fagus orientalis L.) wood
Authors: Taghiyari Hamid R. , Antov Petar , Soltani Abolfazl , Ilies Dorina Camelia , Nadali Elham , Lee Seng Hua , Grama Vasile , Simona Tripa
Published: February 2024

Sepiolite is a silicate mineral that improves the fire properties in solid wood when mixed with a water-based coating. The present study was carried out to investigate and evaluate the effects of sepiolite addition to acrylic-latex paint on the pull-off adhesion strength, as an important characteristic of paints and finishes used in the modern furniture industry and historical furniture as well for preservation and restoration of heritage objects. Sepiolite was added at the rate of 10%, and brushed onto plain-sawn beech (Fagus orientalis L.) wood specimens, unimpregnated and impregnated with a 400 ppm silver nano-suspension, which were further thermally modified at 185 °C for 4 h. The results showed that thermal modification had a decreasing effect on the pull-off adhesion strength, primarily as a result of the thermal degradation of cell-wall polymers (mostly hemicelluloses). Still, a decreased wettability as a result of condensation and plasticization of lignin was also partially influential. Based on the obtained results,thermal modification was found to have a significant influence on pull-off adhesion strength. Sepiolite addition had a decreasing effectin all treatments, though the effect was not statistically significant in all treatments. The maximum and minimum decreases due to sepiolite addition were observed in the unimpregnated control (21%) and the thermally-modified NS-impregnated (4%) specimens. Other aspects of the sepiolite addition, and further studies that cover different types of paints and coatings, should be evaluated before coming to a final firm conclusion in this regard.
Numerical simulation and tool parameters optimization of aluminum alloy transmission intermediate shell
Authors: Zhao Haiyue , Cao Yan , Bai Yu , Yao Hui , Tian Chunlei
Published: February 2024

Due to its challenging manufacturing and intricate morphology, the aluminum alloy transmission intermediate shell used in vehicle transmission has been the focus of many academic studies. In this study, the three-dimensional cutting model is condensed to a two-dimensional cutting model and utilized to simulate the finishing process of an aluminum alloy workpiece using the finite element modeling program DEFORM-3D. Through orthogonal testing and range analysis, the impact of integral end mill side edge parameters on cutting performance was investigated. It is determined that tool chamfering has a greater impact on cutting performance than tool rake and relief angles, that chamfering width has the most impact on cutting force, and that chamfering angle has the greatest impact on cutting temperature. The workpiece's surface roughness is tested during a cutting experiment, and an analysis of the data reveals that the finite element simulation model is accurate and the orthogonal test method is reasonable. The tool chamfer has a greater impact on roughness than the tool rake angle and relief angle. The tool settings are further optimized using the firefly method. By examining the data, it is determined that the prediction model is correct and the optimization model is reasonable. The cutting efficiency is higher and the surface quality is better when the chamfer width is 0.17 mm and the chamfer angle is 7.3° or 18.3°. Therefore, optimizing the side edge parameters of the integral end mill during the finishing process of a thin-walled aluminum alloy shell has practical technical value.
Neutrophil to lymphocyte ratio may predict efficacy of anti-PD-1 inhibitors in advanced EGFR-mutant non-small cell lung cancer: retrospective cohort study
Authors: Chen Jianxin , Zheng Qinhong , Zhu Shijian , Qiu Dan , Wang Junhui
Published: February 2024

This study aimed to investigate the associations between the clinical characteristics and effectiveness of anti-PD-1 inhibitors in patients with EGFR-sensitive mutations, aiming to identify the potential subgroup of patients who might benefit from anti-PD-1 inhibitor treatment. Patients with advanced non-small cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR)-sensitive mutations who received subsequent anti-PD-1 inhibitors in combination with chemotherapy/antiangiogenic agents or alone after progression to tyrosine kinase inhibitors (TKIs) were screened. Clinical characteristics, including hematological parameters, were investigated for potential correlations with clinical outcomes. Subgroup and multivariate analyses were used for further confirmation of the relationship. Kaplan–Meier curves and Cox survival regression models using the log-rank test were used for progression-free survival (PFS) and overall survival (OS) assessments between the groups. Multiple regression analysis was performed using the standard regression coefficient values. The Wilcoxon test was used for the analysis of the variation in NLR. P ≤ 0.05 was considered to indicate statistical significance. This study was a retrospective study. Twenty-two patients met the inclusion criteria and were included in the study. The median PFS was 3.05 months (95% CI, 2.9–10.2 months). The median OS was 7.30 months (95% CI, 5.2–18.1 months). PFS in low neutrophil to lymphocyte ratio (NLR ≤ 4) was significantly longer than high NLR (NLR > 4, 5.7 months versus 2.0 months, HR, 0.35, 95% CI, 0.08–0.63, P = 0.0083). The OS in the low NLR group was also significantly better than that in the high NLR group (OS, 21.3 months versus 5.0 months, HR, 0.33; 95% CI, 0.09–0.74; P = 0.0163). In the multivariate analysis, NLR was the only significant factor for OS benefits (β = 3.535, 95% CI, 1.175–10.636, P = 0.025). Further investigation revealed that front-line TKIs exposure may contribute to the elevation or decrease of NLR, and finally lead to different efficacy outcomes by anti-PD-1 inhibitors. The findings suggest that a portion of advanced NSCLC patients with low NLR characteristics (NLR ≤ 4), even those harboring EGFR-sensitive mutations, could benefit from anti-PD-1 inhibitors as further line treatment after progression to EGFR-TKIs.
Quantification of ischemic brain edema after mechanical thrombectomy using dual-energy computed tomography in patients with ischemic stroke
Authors: Steffen Paul , Winkelmeier Laurens , Kniep Helge , Geest Vincent , Soltanipanah Setareh , Fiehler Jens , Broocks Gabriel
Published: February 2024

Net water uptake (NWU) is a quantitative imaging biomarker used to assess cerebral edema resulting from ischemia via Computed Tomography (CT)-densitometry. It serves as a strong predictor of clinical outcome. Nevertheless, NWU measurements on follow-up CT scans after mechanical thrombectomy (MT) can be affected by contrast staining. To improve the accuracy of edema estimation, virtual non-contrast images (VNC-I) from dual-energy CT scans (DECT) were compared to conventional polychromatic CT images (CP-I) in this study. We examined NWU measurements derived from VNC-I and CP-I to assess their agreement and predictive value in clinical outcome. 88 consecutive patients who received DECT as follow-up after MT were included. NWU was quantified on CP-I (cNWU) and VNC-I (vNWU). The clinical endpoint was functional independence at discharge. cNWU and vNWU were highly correlated (r = 0.71, p < 0.0001). The median difference between cNWU and vNWU was 8.7% (IQR: 4.5–14.1%), associated with successful vessel recanalization (mTICI2b-3) (ß: 11.6%, 95% CI 2.9–23.0%, p = 0.04), and age (ß: 4.2%, 95% CI 1.3–7.0%, p = 0.005). The diagnostic accuracy to classify outcome between cNWU and vNWU was similar (AUC:0.78 versus 0.77). Although there was an 8.7% median difference, indicating potential edema underestimation on CP-I, it did not have short-term clinical implications.
A cooperative optimization method for the layout of shared bicycle parking areas and delivery quantity
Authors: Chen Hao , Wang Wenxian , Cheng Ligang , Li Ping
Published: February 2024

With the popularity of shared bicycles in urban areas, more and more residents choose this fast and convenient mode of transportation for short-distance travel. By optimizing the layout of shared bicycle parking areas and delivery quantity, the investment cost of shared bicycle enterprises can be effectively reduced, and the convenience of residents' travel can be improved at the same time. In this paper, we develop a collaborative optimization model for the layout of the shared bicycle parking area and delivery quantity, aiming at minimizing the walking distance of residents and the investment cost of enterprises, while considering the constraints of the parking area's attractive range and the number of bicycles placed. Aiming at the characteristics of this mixed integer nonlinear problem, an improved genetic algorithm incorporating symmetric individual precision control mechanism is designed. Finally, taking the planned area between the Second Ring Road and the Third Ring Road in the northern part of Jin-niu District, Chengdu as the background, the proposed collaborative optimization model for the layout of shared bicycle parking areas and delivery quantity is applied to a real scene. The results show that after optimization, the number of parking areas is reduced by 2, and the total investment cost is reduced by about 12.2%.