bluuebunny/crossref_metadata_2025 huggingface dataset:
An approach to genetic testing in patients with metastatic castration-resistant prostate cancer in Singapore
Authors: Kanesvaran Ravindran , Chia Puey Ling , Chiong Edmund , Chua Melvin Lee Kiang , Ngo Nye Thane , Ow Samuel , Sim Hong Gee , Tan Min-Han , Tay Kiang Hiong , Wong Alvin Seng Cheong , Wong Siew Wei , Tan Puay Hoon
Published: March 2023

Introduction: There has been a rapid evolution in the treatment strategies for metastatic castration-resistant prostate cancer (mCRPC) following the identification of targetable mutations, making genetic testing essential for patient selection. Although several international guidelines recommend genetic testing for patients with mCRPC, there is a lack of locally endorsed clinical practice guidelines in Singapore.
Method: A multidisciplinary specialist panel with representation from medical and radiation oncology, urology, pathology, interventional radiology, and medical genetics discussed the challenges associated with patient selection, genetic counselling and sample processing in mCRPC.
Results: A clinical model for incorporating genetic testing into routine clinical practice in Singapore was formulated. Tumour testing with an assay that is able to detect both somatic and germline mutations should be utilised. The panel also recommended the “mainstreaming” approach for genetic counselling in which pre-test counselling is conducted by the managing clinician and post-test discussion with a genetic counsellor, to alleviate the bottlenecks at genetic counselling stage in Singapore. The need for training of clinicians to provide pre-test genetic counselling and educating the laboratory personnel for appropriate sample processing that facilitates downstream genetic testing was recognised. Molecular tumour boards and multidisciplinary discussions are recommended to guide therapeutic decisions in mCRPC. The panel also highlighted the issue of reimbursement for genetic testing to reduce patient-borne costs and increase the reach of genetic testing among this patient population.
Conclusion: This article aims to provide strategic and implementable recommendations to overcome the challenges in genetic testing for patients with mCRPC in Singapore.

Keywords: Clinical model, genetic counselling, genetic testing, homologous recombination repair genes, metastatic castration-resistant prostate cancer
Determinants of the Success in Tasks of Indonesian Army Personnel in the UN Peace Mission in Lebanon
Authors:   Associate Professor, Department of Management, Faculty of Economics and Business, Universitas Malikussaleh, Aceh, Indonesia, Matriadi Faisal , Arifin AHadi ,   Professor, Department of Management, Faculty of Economics and Business, Universitas Malikussaleh, Aceh, Indonesia, Ginting Edy Saputra ,   Indonesian Army Personnel (TNI AD) who joined the UNIFIL peace mission in Lebanon and Magister student at the Faculty of Economics and Business, Universitas Malikussaleh, Aceh, Indonesia, Ikramuddin Ikramuddin ,   Doctor of Management, Department of Management, Faculty of Economics and Business, Universitas Malikussaleh, Aceh, Indonesia
Published: March 2023

This study examines and analyzes the Influence of Discipline and Workplace Spirituality on the success of the tasks of Indonesian Army Personnel in the U.N. peacekeeping mission in Lebanon, with motivation as an Intervening Variable. The respondents used are 136 members of the Indonesian Army in Lebanon taken using the Census sampling method. The data analysis method used is Structural Equation Modeling (SEM) using AMOS 21. The results indicate that discipline and workplace spirituality positively and significantly influence work motivation and the success of the tasks of Indonesian Army Personnel, and work motivation positively and significantly influence the success of the duties of Indonesian Army Personnel. The results of the mediation effect test prove that work motivation can partially mediate the influence of discipline and workplace spirituality on the success in the tasks of Indonesian Army Personnel as members of the U.N. peacekeeping forces in Lebanon.
Tendency of Accounting Fraud from Aspect Internal Control, Compatibility of Compensation and Individual Morality
Authors:   Faculty of Economy and Business Universitas Muhhamaddiyah Palembang, Sania Sunardi dan
Published: March 2023

Testing the tendency of accounting fraud from the aspect of internal control, appropriateness of compensation and individual morality needs to be proven through research. The type of research used is associative. The location of the research was at 7 State-Owned Enterprises (BUMN) in Palembang City. The data used were primary data. Data collection techniques were through interviews and questionnaires. The sample collection method used a saturated sample. The population in this study were internal auditors and financial employees as many as 35 respondents. The data analysis method used was quantitative. The results showed that partially internal control and individual morality had no effect on the tendency of accounting fraud, then the appropriateness of compensation had a significant effect on the tendency of accounting fraud.
MEDICAL MANAGEMENT OF BROKEN GUIDEWIRE: CASE REPORT
Authors:   Cardiology Department A Ibn Sina Hospital, NADIA FELLAT , NASSIMA EL KARROUMI ,   Cardiology Department A Ibn Sina Hospital, KENZA ALAMI ,   Cardiology Department A Ibn Sina Hospital, ROKAYA FELLAT ,   Cardiology Department A Ibn Sina Hospital
Published: March 2023

Bifurcation lesions were once indicating surgery but not anymore, now they will follow the same principles for revascularization of non-bifurcation lesions. However, PCI of bifurcation lesions can be world widely challenging due to anatomic characteristics of each lesion. Guidewire fracture is known to be a rare complication of percutaneous coronary intervention. The consequences however might be deadly, and its management is still debated until further guidelines. The aim of this article is to raise the difficulties of the interventional care of the LAD/Diagonal lesions.
Islam Wasathiyah
Authors: Pajarianto Hadi
Published: March 2023

Wasatiyah Islam is a religious interpretation with the principles of justice, tolerance, and upholding humanity despite different religions, ethnicities, ethnicities and customs. This is the face of Islam that Muhammadiyah believes in and implements in its various charities. The aims of this study were (i) to identify how non-Muslim students perceive religious education materials; (ii) to analyze the behavior of lecturers in displaying Islamic wasathiyah; and (iii) to analyze the interaction of Muslim and non-Muslim students in the implementation of Wasathiyah Islam. This research is descriptive and quantitative by utilizing the frequency distribution. Respondents in this study were 25 non-Muslim students at the Muhammadiyah University of Palopo. The research results show that; first, non-Muslim students have a good perception of AIK material, which has included wasatiyah principles in it. Second, the behavior of lecturers has shown fairness and equality in granting rights to Muslim and non-Muslim students, according to wasatiyah principles. Third, the interaction of Muslim and non-Muslim students takes place with the principles of equality and justice. The implication of the results of this research is that every educational institution must be open to adherents of other religions to continue their studies. With the principles of Wasatiyah Islam, religious education can be carried out, even for non-Muslim students

Keywords: Islam, Wasatiyah, Religious Education, Non-Muslim, Muhammadiyah
Management Support Practices on the Department of Education Reading Program
Authors: Paro Dayrocas Mary Grace
Published: March 2023

This case study explores the management support practices of the Department of Education Reading Program in Panabo City Division, Davao Del Norte, Philippines. Seven (7) school principals were interviewed using Key Informants Interview, and another seven (7) school reading teachers were invited to the Focus Group Discussion using the same enhanced researcher–made interview guide. Findings revealed that the Department of Education manifested management support practices on the reading program, such as policies, teachers, external stakeholders, learning spaces, and learning resources, which were perceived to be consistent with general system theory of Ludwig von Bertalanffy (1928). Each input has a unique process, output, and outcome in this study. This paper provided implications for practice and further research on management support in a reading program.
Prevalence of Clostridium perfringens and Clostridium difficile carrying cpa, tcdA toxin genes in raw meat and leafy vegetables
Authors: Ta Thi Yen , Ninh Thi Hanh , Vu Thi Hai Ha , Pham Van Quan , Nguyen Thanh Trung ,   National Institute for Food Control, Hanoi, Vietnam,   National Institute for Food Control, Hanoi, Vietnam,   National Institute for Food Control, Hanoi, Vietnam,   National Institute for Food Control, Hanoi, Vietnam,   National Institute for Food Control, Hanoi, Vietnam
Published: March 2023

The genus Clostridium is a Gram-positive, spore-forming bacterium. Most species of Clostridium spp. usually are found in soil, water, dead plants, animal carcasses, and play an important role in the decomposition of substances in nature. Raw meat and fresh vegetable are more likely to be contaminated with spores or vegetative cells of the genus Clostridium. The study results showed that the percentage of samples contaminated with raw meat and leafy vegetables collected at markets in Dong Da, Ha Dong and Hoang Mai districts infected with C. perfringens was 26.67% (n = 150) and 26.82% (n = 220). The percentage of raw meat and leafy vegetable samples contaminated with C. difficile was very low with 6.00% (n = 150) and 0.9% (n = 220), respectively. Gene amplification reactions revealed the presence of cpa toxin gene in all C. perfringens strains and detected 02 C. difficile strains carrying the tcdA gene.
Plant Life: An Intelligent Mobile Plant Disease Diagnostic System using Deep Learning
Authors: Dr. Muthu Senthil B  , Mummaneni Sravani  , Konduru Theja Sree  , Krithik Shri M. P.
Published: March 2023

Agriculture is an important sector that considerably enhances a nation's economic development. Farmers encounter several obstacles every year in order to produce high-quality crops. Plant diseases are a significant factor in the failure of the harvest. Additionally, pests, specialized illnesses, or natural disasters cause massive crop losses every year. It raises serious concerns about the application of sustainable advanced technologies to agricultural problems. The proposed intelligent system's main job is to detect plant disease using an image captured in the mobile application. It employs the region-based convolution neural network (fast-RCNN) and pre-trained model generation for the set of datasets and captured images. To ensure long-term viability, the mobile app is integrated with cloud services. The proposed system also includes recommendations to help farmers determine whether plant conditions are healthy or unhealthy, as well asthe recommendations to cure disease.
Aparentemente inamovibles e incorruptibles
Authors: Pastor Andrés Gema , Guerra Antonio
Published: March 2023

Este ensayo propone un diálogo entre un texto, en forma de tragedia griega clásica, escrito por Gema Pastor Andrés y una serie fotografías que forman parte del fotolibro La luz que nos ciega de Antonio Guerra. Ambas propuestas hablan de la creación, la muerte, la percepción, la sensación y la representación, la tecnología, el arte… pero, sobre todo, acerca del peso del lugar que las ha inspirado, la Castilla contemporánea, despoblada y desertificada por el fuego, el hielo, la producción agrícola y la densidad de su cultura.
Bomba de miel en el lugar de trabajo, plástica social en el museo de arte
Authors: Gutiérrez Galindo Blanca
Published: March 2023

Uno de los proyectos más significativos del artista alemán Joseph Beuys ((1921-1986) es Bomba de Miel en el lugar de trabajo, realizado en 1977 en conjunto con los miembros de la Universidad Internacional Libre, una iniciativa ciudadana creada por el artista como foro internacional permanente abierto a la discusión de los problemas sociales. El objetivo de este texto es analizar ese proyecto en el marco de las ideas de arte ampliado y plástica social que animaron la actividad artística de Beuys a partir de los años setenta. Mostraré su importancia como parte de una redefinición neovanguardista del arte en el contexto de una Alemania Occidental dividida por la Guerra Fría y en plena efervescencia ciudadana.
The Luminous Horizon of Le Corbusier’s Last Project
Authors: Klein Richard
Published: March 2023

Le Corbusier's project for the Congress Hall for the city of Strasbourg between 1962 and 1965 has a relatively discreet place in historiography. However, it is frequently presented as a synthesis that skilfully blends the archaism of the rough finishes with the new machinist features of the 1960s. The evolution of the last design phases shows how the spectacular and event-based dimensions largely take precedence over the usual practices of the rue de Sèvres workshop.
LC150+, a conversation with Rene Tan, Jonathan Quek, Ian Soon, Keefe Chooi and Jannelle Ho
Authors: Lapunzina Alejandro
Published: March 2023

Interview
Estrategia de control selectivo de temperatura y humedad para un invernadero solar chino con un enfoque basado en eventos
Authors: Liu Ran , Guzmán José Luis , García-Mañas Francisco , Li Ming
Published: March 2023

Este artículo presenta la aplicación de un esquema de control selectivo de temperatura y humedad para invernaderos solares chinos, que son los más utilizados en las provincias del norte de China. En primer lugar, para controlar la temperatura, se propone un controlador PI con un enfoque basado en eventos. Tras la evaluación de varios valores de la banda de ocurrencia de eventos, se obtiene una solución que permite reducir en un 43,8%el número de aperturas y cierres de las ventanas del invernadero,  mientras que el error de temperatura se incrementa sólo en un 1,13 %. En segundo lugar, se ha diseñado un  controlador para la humedad relativa y otro para la humedad absoluta. Los resultados muestran que el control de humedad relativa funciona adecuadamente cuando la consigna no es demasiado elevada. Sin embargo, la acción de control se deteriora cuando la consigna es superior al 70 %. En comparación, el control de humedad absoluta permite regular la humedad para referencias de cualquier valor, pero la precisión de control es menor. Finalmente, mediante un estudio en simulación, se demuestra la efectividad de la estrategia de control selectivo de temperatura con un esquema que da prioridad para controlar la humedad cuando ésta alcanza límites no deseados. Esta estrategia de control consigue mantener la humedad relativa por debajo del 80% mientras que controla la temperatura en la consigna establecida, evitando así que la alta humedad dañe al cultivo.
Manipulación visual-táctil para la recogida de residuos domésticos en exteriores
Authors: Castaño-Amorós Julio , Páez-Ubieta Ignacio de Loyola , Gil Pablo , Puente Santiago Timoteo
Published: March 2023

Este artículo presenta un sistema de percepcion orientado a la manipulación robótica, capaz de asistir en tareas de navegación, clasificacion y recogida de residuos domésticos en exterior. El sistema está compuesto de sensores táctiles ópticos, cámaras RGBD y un LiDAR. Estos se integran en una plataforma móvil que transporta un robot manipulador con pinza. El sistema consta de tres modulos software, dos visuales y uno táctil. Los módulos visuales implementan arquitecturas CNNs para la localización y reconocimiento de residuos sólidos, además de estimar puntos de agarre. El módulo táctil, también basado en CNNs y procesamiento de imagen, regula la apertura de la pinza para controlar el agarre a partir de informacion de contacto. Nuestra propuesta tiene errores de localizacion entorno al 6 %, una precisión de reconocimiento del 98 %, y garantiza estabilidad de agarre el 91 % de las veces. Los tres modulos trabajan en tiempos inferiores a los 750 ms.
Effect of group size and escape enrichment on reproductive performance of breeding does in part-time group housing
Authors: Van Damme Liesbeth G. W. , Delezie Evelyne , Maertens Luc , Ampe Bart , Tuyttens Frank A. M.
Published: March 2023

Societal demands for group housing of social farm animals such as rabbits are increasing due to animal welfare concerns. When breeding does are housed in groups, maternal protective behaviour negatively affects the reproductive performance of the does. In part-time group housing, does are housed in single-litter cages starting shortly before parturition until the first part of lactation and then group housed for the second part after their protective behaviour has diminished and the kits are more resilient. The aim of this study was to evaluate the reproductive performance of breeding does in a part-time group housing system with provision of escape enrichment and different group size (and concomitant different stocking density, as pen size remained unchanged). For each of the four consecutive reproductive cycles, 42 does with their kits switched at 22 d post-partum (pp) from single-litter to group housing for a total period of 13 d in pens of 200×102 cm by removing walls between four adjacent single-litter cages. Newly created group pens (N =12 pens per treatment) varied in group size, stocking density and enrichment: groups of either three or four does were divided over same-size pens with or without escape enrichment (2×2 factorial design). The escape enrichment was comprised of two sets of PVC pipes and two extra elevated platforms. Does were weighed and kits counted at parturition, after which kits were cross-fostered. Kits were counted and weighed again 22 and 35 d pp. Results for mean doe fertility (90.0%), number of kits (9.1 kits/litter) and kit weight (1037.3 g/kit) at weaning were comparable with professional farms using single-litter cages. No significant effects of group size and escape enrichment were found for any of the reproductive parameters. While housed in group, seven does were removed from the experiment, from which four does were severely injured. Post-grouping kit mortality was rather low (6.7%), but three kits were euthanised due to severe injuries. In conclusion, provision of escape enrichment and altered group size (and stocking density) had no profound effect on the reproductive performance of part-time group housed does.
Measuring the economic performance of small-scale rabbit production agribusiness enterprises
Authors: Mukaila Ridwan
Published: March 2023

Reducing malnutrition and poverty remains at the centre of policy. Rabbit rearing, of great economic importance, is a critical pathway to achieving this. Good knowledge of the profitability of rabbit production and its driving factors can enhance participation in rabbit production. Thus, this study examined the economic performance (profitability) of rabbit production, the factors influencing profitability and its barriers. Descriptive statistics, profitability analysis, the Tobit regression model and Garret ranking were employed to achieve the objectives. The results indicated that rabbit production was economical, productive and profitable, with a gross margin of N675,990 (USD 1,633.5), a net income of N663,974 (USD 1,604.4), a profit ratio of 0.6, a benefit-cost ratio of 2.7, a return on capital invested of 1.7 and an operating ratio of 0.4. The factors that enhanced rabbit production profitability were stock size, education, experience, membership of the association and labour availability, whereas mortality, disease outbreaks and feeding costs were inhibiting factors to profitability. The major constraints affecting rabbit production are disease, a high mortality rate and poor access to credit. These call for the provision of disease management training and credits to motivate people to engage in rabbit farming, which will, in turn, lower poverty and increase protein availability.
All-round counterattack to conquer lung cancer
Authors: Jang Seung Hun
Published: March 2023

Background: Lung cancer is increasing exponentially as the population ages. To conquer lung cancer, early diagnosis, developing new treatments, and combining multidisciplinary treatment modalities are essential.Current Concepts: Since the national lung cancer screening program for high-risk individuals using low-dose chest computed tomography has launched, the rate of early diagnosis of lung cancer is expected to increase. The development of immune checkpoint inhibitors and target agents is paying off in terms of producing new anticancer drugs. Immune checkpoint inhibitors are administered in combination with existing treatment modalities in various clinical situations, such as for not only patients with metastatic lung cancer but also for those with resectable lung cancer and with surgically unresectable locally advanced disease. These trials dramatically improved survival outcomes. The development of targeted anticancer drugs is also advancing at a rapid pace. The survival rate of patients with lung cancer who have specific gene mutations has greatly improved when targeted anticancer drugs are administered alone or in combination with conventional therapies.Discussion and Conclusion: Early diagnosis of lung cancer and the development of new treatment modalities are greatly improving the prognosis of patients with lung cancer. Attempts to combine conventional and new treatment modalities should continue. It is necessary to discuss changing medical policies for long-term survivors, which will inevitably increase.
New targeted therapies for non-small cell lung cancer
Authors: Lee Eun Hye , Kwak Se Hyun , Kim Chi Young , Gwon Hye Ran , Kim Eun Young , Chang Yoon Soo
Published: March 2023

Background: Lung cancer is representative of a high frequency and high mortality disease and ranks at the top in incidence and mortality in Korea, when excluding thyroid cancer. In this manuscript, we focused on current targeted therapies for lung cancer used widely in the medical field.Current Concepts: The majority of patients with lung cancer cannot be treated with surgery only and require chemotherapeutics or radiation therapy. Currently, classical chemotherapeutic agents, targeted agents, and immune checkpoint inhibitors are the most widely used. Recently, the Research and Development of antibody-drug conjugates is gaining attention, and this may become a more widely prescribed treatment in the future. Among the available treatment options, targeted therapy is becoming increasingly feasible and widespread for treating inoperable lung cancers, where driver mutations have been identified, and for adjuvant or neoadjuvant therapies. Next-generation sequencing (NGS) improves the ability to identify driver mutations that were previously difficult to detect and can also be performed on blood samples where no cancer tissue is available for testing. This makes it possible to identify therapeutic targets for targeted therapy more rapidly.Discussion and Conclusion: The most common type of lung cancer in Korea is adenocarcinoma, for which a driver mutation has been identified. Newly developed drugs target previously problematic mutations or cancer cell lines that have acquired resistance induced during the treatment process. The survival rate of patients with lung cancer is expected to improve with the development of tailored treatments for targets identified from the NGS data of the patient. This paper will help clinicians understand the current state of targeted therapies for lung cancer treatment.
Descriptive analysis of the incidence rate of post-acute COVID-19 syndrome in the Republic of Korea Army
Authors: Kim Taejae , Ha Beomman , Yang Ji-in , Kim Mi-Jung , Seo Kyung-Wan
Published: March 2023

Background: This study aimed to identify the incidence rate of post-coronavirus disease-2019 (COVID-19) conditions in the Republic of Korea (ROK) Army and to investigate the trend of the incidence rate according to changes in dominant variants.Methods: We used the results of a 19-item, self-completed survey of those who had recovered from COVID-19 in the ROK Army between March 24, 2020, and April 30, 2022. We used both descriptive and multiple logistic regression analyses to identify factors associated with the incidence rate of post-COVID-19 conditions.Results: Among the total of 48,623 COVID-19 episodes in the ROK Army, the overall incidence rate of post-COVID-19 conditions was 32.9%. Based on the survey, the incidence of cough was the highest at 15.4%, followed by fatigue (15.1%) and sputum (13.8%). The delta variant had the highest incidence rate of post-COVID-19 conditions at 50.7%, whereas the omicron variant had the lowest at 19.7%. Concerning the type of post-COVID-19 condition, the neuropsychiatric symptoms had the highest incidence at 27.4% when the delta variant was dominant, and the respiratory symptoms were highest at 37.3% when the omicron variant was dominant. In the case of smell and taste symptoms, the incidence rate was high at 21.1% only when the delta variant was predominant.Conclusion: The overall incidence rate of post-COVID-19 conditions in the ROK Army was 32.9%. When the delta variant was dominant, the overall incidence as well as the proportion of neuropsychiatric symptoms were high. However, as the omicron variant became dominant, the overall incidence decreased, but the proportion of respiratory symptoms increased.
Problems of patriotic education and forms of its implementation in educational institutions of higher education of law enforcement agencies of Russia
Authors: Реент Ю.А.
Published: March 2023

В статье освещаются теоретические основы и практический опыт формирования патриотических убеждений в образовательных организациях высшего образования, в первую очередь – Федеральной службы исполнения наказаний. Предметом исследования определены процессы патриотического воспитания в социально-педагогических условиях. Целью статьи является оценка общих педагогических и организационных наработок, как исторически сложившихся в отечественных учебных заведениях, осуществляющих подготовку сотрудников правоохранительных органов, так и формирующихся вновь в современных социально-политических условиях. Высказываемые рекомендации и выводы базируются на анализе материалов, содержащихся в ряде государственных нормативно-правовых актов и реализуемых в практической деятельности сотрудников уголовно-исполнительной системы. Указывается, что эта работа является сферой ответственности не только педагогики, психологии или теории образования, но истории, философии права и ряда иных научных направлений, учебных дисциплин.

The article highlights the theoretical foundations and practical experience in the formation of patriotic beliefs in educational institutions of higher education, in the first place - the Federal Penitentiary Service. The subject of the research is the processes of patriotic education in socio-pedagogical conditions. The purpose of the article is to assess the general pedagogical and organizational developments, both historically established in domestic educational institutions that train law enforcement officers, and emerging again in modern sociopolitical conditions. The recommendations and conclusions made are based on the analysis of the materials contained in a number of state regulations and implemented in the practical activities of the employees of the penal system. It is indicated that this work is the responsibility of not only pedagogy, psychology or the theory of education, but history, philosophy of law and a number of other scientific areas, academic disciplines.
Molecular genetic markers of resistance and virulence of invasive Klebsiella pneumoniae strains according to whole genome sequencing data
Authors: Bonda N. A. , Stoma I. О. , Osipkina O. V. , Ziatskov A. A. , Shaforost A. S. , Karpova E. V. , Tapalski D. V.
Published: March 2023

Objective. To evaluate genetic mechanisms of antibiotic resistance and virulence of invasive strains of Klebsiella pneumoniae isolated from inpatients using whole genome sequencing.

Materials and methods. For two carbapenem-resistant multiple-antibiotic-resistant invasive strains of K.pneumoniae, as well as two carbapenem-sensitive invasive strains of K.pneumoniae, sequencing was performed using the MiSeq genomic sequencer (Illumina). Genomic sequences were assembled and annotated. Sequence type determination, search for plasmids and virulence factors, antibiotic resistance genes, and efflux mechanisms were performed.

Results. K.pneumoniae strains belonged to sequence types ST395, ST101, ST111, and ST512 s and had a hypermucoid phenotype. The iutA aerobactin genes were detected in both sensitive and carbapenem-resistant strains. Virulence genes fimH, fyuA, and irp2 were detected in one strain isolated from blood. Carbapenemase genes (blaKPC, blaNDM) were detected in two strains. Aminoglycosides and fluoroquinolones resistance genes were detected in 3 of 4 strains. All strains showed the presence of different systems of active antibiotic elimination from the microbial cell.

Conclusion. The possibility of identifying hypervirulent strains of K.pneumoniae using a complex phenotypic test along with hvKp genotyping is shown. The results of full-genome sequencing reflect significant resistance of hypervirulent K.pneumoniae strains isolated from blood to most antibiotics, including β-lactams, aminoglycosides, fluoroquinolones, phosphomycin, chloramphenicol and polymyxins.
Cytological method of research in the diagnosis of urinary pathology in liver cirrhosis
Authors: Malaeva E. G. , Zaitseva L. P. , Knyazyuk A. S. , Osipkina O. V.
Published: March 2023

Objective. To determine the possibilities of cytological method of urine cellular sediment examination for diagnosis and differential diagnosis of renal and urinary tract pathology in liver cirrhosis.

Materials and methods. A standard observational study of 50 inpatients with liver cirrhosis was performed. Cytological preparations of urine sediment were prepared by liquid cytology and cytocentrifugation methods and after fixation were 

Results. Cytological study of cellular urine sediment in hospitalized patients with cirrhosis revealed dysmorphic (leached) erythrocytes in 31% of cases, statistically significantly more frequently in patients with decompensation of the disease and 30-day mortality (p<0,05). Microflora in the urine was found in 63% of patients, more often of mixed nature (27%). In 46% of patients with decompensated liver cirrhosis, including acute renal injury, cytological preparation of urinary sediment is represented by multicellular elements with the presence of superficial and deep urothelium and renal epithelium.

Conclusions. Cytological method of studying the cellular urine sediment is an accessible, informative and cheap diagnostic method, the possibilities of which are to determine the presence and origin of cellular elements (urothelium of the surface and deep layers, altered erythrocytes), microflora and its source (genital tract, urinary tract), which, in combination with other methods, allows to verify the diagnosis of the patient and to carry out differential diagnosis of acute tubular necrosis and hepatorenal syndrome.
Clinical-immunological and neurophysiological correlations in paraneoplastic polyneuropathy caused by small cell lung cancer
Authors: Ponomarev V. V. , Reduto E. V.
Published: March 2023

Objective. To study the correlation between the neurological, immunological and neurophysiological signs of paraneoplastic polyneuropathy (PPNP) caused by small cell lung cancer (SCLC), to improve the quality of diagnosis of this pathology.

Materials and methods. Clinical, immunological (anti-Hu) and neurophysiological (electromyography (EMG), electroneuromyography (ENMG), somatosensory evoked potentials (SSEP)) examination of 61 patients with PPNP caused by SCLC are presented.

Results. It was established that sensory disorders in the limbs are the first and obligate clinical symptoms of PPNP, which precede the initial diagnosis of SCLC in 56% in 3-12 months, characterized by a predominance of a decrease in surface sensitivity in the form of distal hypoesthesia in 70% of cases. The motor form is characterized by an older age (67 years) than the sensory (60 years) and sensorimotor (58.0 years, p <0,05). Motor disorders in the limbs occur in 46% of cases, manifested by mild symmetrical distal paresis of the legs and subclinical damage to the motor nerves of the upper extremities according to the results of ENMG (p <0,001). Detection of anti-Hu in plasma is highly correlated with a significant increase in latency and a decrease in amplitudes component N22 in the conduct of SSEP, which indicates the defeat of the axons of the sensory nerves of the limbs (p <0,05). Changes in amplitude parameters, with preserved speed indicators of nerves according to the results of ENMG indicate an axonal type of lesion (p <0,05), which, in combination with the detection of anti-Hu in plasma, are pathognomonic signs of the paraneoplastic nature of the process.

Conclusion. As a result of the study, clinical, immunological and neurophysiological features of various forms of PPNP were identified. The obtained correlations make it possible to diagnose the corresponding type of nerve pathology more accurately and in a timely manner and to suspect the development of oncological disease in time. The revealed changes in the indicators of the N22 component of SSEP can serve as a marker of axonal lesion of peripheral nerves, which with high diagnostic efficiency justifies the expediency of inclusion in the protocol of the ENMG study for verification of additional signs characteristic of PPNP.
Surgical anatomy of the intrapelvic branches of the superior gluteal artery in people of a mesohomorphic somatotype
Authors: Kuzmenko A. V. , Zhdanovich V. N.
Published: March 2023

Objectives. To explore data on branching variants of the intrapelvic part of the superior gluteal artery in people of mesomorphic somatotype.

Materials and methods. We used 48 male dead bodies (deceased aged 25 to 82) and 30 female dead bodies (deceased aged 28 to 78) whose deaths were the result of accidental causes unrelated to the pelvic organs pathology. The following methods were used to achieve the goal of the study: vascular injection, dissection, statistical processing.

Results. The pattern of localization of anastomotic branches of the intrapelvic part of the superior gluteal artery (SGA) in humans of both sexes of mesomorphic somatotype was established for the first time. In men, 59.1% of all anastomotic branches of the SGA are located in the proximal third, 36.4% of anastomoses are in the middle third of this artery, and 4.5% of arterial connections are in the distal third of a. glutea superior. In women, arterial anastomoses branch from the proximal third of the intrapelvic portion of the SGA in 66.6% of cases and in the middle third of this artery in 33.3% of cases. The SGA in men of mesomorphic somatotype anastomoses more frequently than in women with the same type of build. It was found that intrathecal branches and anastomoses of the SGA in men and women of mesomorphic somatotype are predominantly localized along the proximal two thirds of this artery length. New data were obtained on the number of branches originating from a. glutea superior in the pelvic cavity, such as the lateral sacral artery (LSA), iliac-lumbar artery (ILA), obturator artery (OA), middle rectus, lower ureteric artery and rami musculares of the SGA. The SGA was found to be the most frequent source of lateral sacral artery formation in the pelvic cavity in men and women of mesomorphic somatotype.

Conclusions. The patterns of branching of the intrathecal part of the SGA are presented. The results obtained are recommended to be used for endovascular occlusion of the SGA to stop bleeding from the damaged distal third of this artery.
Pathomorphological changes in the organs of laboratory animals with experimental liver cirrhosis
Authors: Evseenko D. A. , Dundarov Z. A. , Nadyrov E. A.
Published: March 2023

Objective. Develop an experimental model of liver cirrhosis and describe pathomorphological changes in organs and tissues of laboratory animals.

Materials and methods. Liver cirrhosis was simulated on 11 sexually mature Wistar male white rats. The control group consisted of 12 healthy laboratory animals. Acute toxic liver injury resulting in cirrhosis was induced by intraperitoneal injection of 50% tetrachloromethane (CCl4) solution on olive oil on the first day of the experiment in the dosage of 0.1 ml CCl4 + 0.4 ml olive oil per 100 g of the animal body weight, on the second day of the experiment 0.3 ml CCl4 + 0.2 ml olive oil per 100 g of the animal body weight. To synergize and potentiate the hepatotoxic effect of CCl4, animals had 10% ethanol solution freely available daily. The duration of the experiment was 65 days. Clinical and laboratory parameters were evaluated, histological evaluation of the preparations was performed. The obtained data were compared with the control group of animals.

Results. Reproducibility of the model was 82% (9 animals). The indicator of lymphocyte blabbing index showed the development of liver cirrhosis in animals of the experimental group. Comprehensive morphological confirmation of the developed liver cirrhosis was obtained.

Conclusion. The proposed methodology of liver cirrhosis formation correlates with pathomorphological changes in lymphocyte cell wall, changes in liver tissue and internal organs in human liver cirrhosis.
Dystrophic changes in the sciatic nerve: a comparison of ultrasonographic and morphological data (pilot study)
Authors: Yurkovskiy A. M. , Pismennikova E. I. , Achinovich S. L.
Published: March 2023

Objective. To study the possibility of using ultrasonography to identify mild (early), moderately severe and significantly severe dystrophic changes in the sciatic nerve.

Materials and methods. To achieve this goal, we compared ultrasonographic and histological data from 15 pairs of sciatic nerves taken from 8 men and 7 women aged 58 (52; 68) years who had been treated for different oncological diseases and died in 2022 from the underlying disease.

Results. Parallelism was revealed between the ultrasonographic pattern in mild, moderately severe and significantly severe dystrophic changes of the sciatic nerve, on the one hand, and the histological and immunohistochemical patterns, on the other hand.

Conclusion: Ultrasonography (B-mode) allows identifying mild, moderately severe and significantly severe dystrophic changes of the sciatic nerve.
Early clinical manifestations of dissecting aneurysm of the thoracic aorta and the importance of their role for the timely diagnosis of the disease
Authors: Kadochkina N. G. , Pronevich A. V.
Published: March 2023

This article presents a clinical case of dissecting thoracic aortic aneurysm. The first clinical manifestations were typical symptoms of the disease. Timely diagnosis and treatment are particularly important to prevent a fatal outcome. Late diagnosis may be related also to the lack of doctors’ caution with regard to dissecting aortic aneurysm.
Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand
Authors: Bahuguna Ananya , Dubey Shiv Kumar , Garia Vaishali , Kumar Ravendra , Prakash Om , Rawat Dharmendra Singh
Published: March 2023

The current study describes the analysis of the phytochemical composition and biological activities of various polarity extracts of the Anaphalis busua plant that was collected at an altitude of 1654 m in the Himalayan terrain of Uttarakhand, India. The extracts were prepared by the cold percolation method, which was then subjected to GC-MS for phytochemical analysis. A total of 31 compounds were identified that constituted 94.95% of the total methanolic extract. Mome inositol (31.03%) was identified as the main compound in the methanolic extract. Twenty-two compounds that comprise 68.24% of the total hexane extract were identified. Tetracontane (19.33%) was present in a significant proportion. The methanolic extract demonstrated potent antioxidant activity in terms of DPPH radical scavenging and metal chelating activity that have IC50 values of 81.71±1.334 and 11.26±0.005 µg/mL, respectively, compared to standards ascorbic acid and EDTA that have IC50 values at 12.71±0.02 and 11.36±0.06 µg/mL, respectively. The methanolic extract showed potent anti-inflammatory activity with an IC50 value of 24.10±0.09 µg/mL in comparison to standard diclofenac potassium with an IC50 value of 18.95±0.03 µg/mL. In vitro studies reveal that A. busua has a strong therapeutic potential and, if further explored, may prove to be a powerful antioxidant, anti-inflammatory, and cost-effective agent compared to synthetically derived agents from pharmaceutical industries.
Biogenic synthesis of selenium nanoparticles using Hibiscus esculentus L. extract: Catalytic degradation of organic dye and its anticancer, antibacterial and antifungal activities
Authors: Ebrahimzadeh Mohammad Ali , Moradsomarein Mina , Lalerdi Fatemeh Sadeghi , Alizadeh Seyedeh Roya
Published: March 2023

In this work, we develop the synthesis of selenium nanoparticles (B@SeNPs) using a green method using the aqueous extract of Hibiscus esculentus L. Various techniques were used to characterize bio-synthesized B@SeNPs. The mixture color was clearly changed to reddish at 45-50 °C and the extract pH = 6. According to Fourier transform infrared spectroscopy (FT-IR), the B@SeNPs were produced, capped, and stabilized using biomolecules found in plant extracts. The energy dispersive X-ray (EDX) analysis profile revealed an atomic Se signal (1.39 mV). The powder X-ray diffraction (PXRD) pattern confirmed the hexagonal phase crystalline form of B@SeNPs. The zeta potential for SeNPs was determined to be -51.3 mV. Scanning electron microscope (SEM) and transmission electron microscopy (TEM) micrographs revealed spherical Se particles with sizes of roughly 62 nm. Furthermore, B@SeNPs can degrade methylene blue dye by 98.3% at 21 min with a rate constant of 0.1023 min-1 in the presence of NaBH4. In biological evaluation, the synthesized nanoparticles have been proven to be effective against two human cancers (AGS and MCF-7 cells) with IC50 values of 20.46 and 88.43 µg/mL, respectively. Additionally, B@SeNPs showed high safety in the Beas cell line (normal) at 123 µg/mL as the highest concentration. The biofabricated SeNPs had a moderate antibacterial effect against ATCC and multidrug-resistant clinical isolates. They had no antifungal activity against the tested fungus strains except C. albicans (IFRC 1873), with a MIC value of 138.75 µg/mL. Finally, the green-synthesized B@SeNPs could be a contender for further testing as a chemotherapeutic agent in the treatment of some human cancers.
Spectroscopic study of solvent effects on the electronic absorption spectra of morpholine and its complexes
Authors: Masoud Mamdouh Saad , Ali Alaa Eldin , Elasala Gehan Shaaban , Elwardany Rehab Elsaid
Published: March 2023

The electronic absorption spectra of morpholine and its five morpholine complexes have been studied in different solvents of various polarities. The regression and correlation coefficients have been calculated with the SPSS program. Solvation energy relationships were deduced from spectral shifts and correlated with solvent parameters α (solvent hydrogen bond donor acidity), β (solvent hydrogen bond acceptor basicity), and π* (dipolarity/polarizability). The percentage contributions of the calculated solvatochromic parameters show that classic solvation effects play a major role in explaining the spectral shifts in all investigated complexes. The blue shift of [Fe(MOR)3Cl3]·4H2O, [Ni(MOR)4Cl2]·4H2O, and [Cu(MOR)4Cl2]·6H2O complexes is due to the formation of hydrogen bonds, which suggests the stabilization of the ground electronic state compared with the excited state. [CuNi(MOR)2Cl4]·4H2O and [CuZn(MOR)3Cl4]·2H2O are mixed metal complexes that suffer a red shift due to the solute-solvent interactions, which causes stabilization of the excited solute state with increasing solvent polarity. The bands are affected by specific solute-solvent interactions including hydrogen bond donor ability (acidity) and hydrogen bond acceptor ability (basicity) and nonspecific solute-solvent interactions including electromagnetic interaction between the dipole moments of solute and polar solvents.
Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy
Authors: Nejres Aws Maseer , Najem Moath Abdallah
Published: March 2023

A new fast and simple selective method for the simultaneous determination of lisinopril dihydrate and amlodipine in combined drugs was developed using the fourth derivative spectrum method, based on the zero-crossing-point technique for the determination of compounds in drugs. The wavelength values for lisinopril dihydrate and amlodipine in solvent medium were found to be (203, 207, and 231 nm) and (215, 254, and 277 nm), respectively, with the average obeying Beer’s law in the range of lisinopril dihydrate 2.0 to 45.0 µg/mL and amlodipine 2.0 to 35.0 µg/mL. Lisinopril dihydrate has molar absorptivity regions (9227.76-11700.28 L/mol.cm, 203 nm), (15320.74-20795.59 L/mol.cm, 207 nm), and (2207.60-3311.40 L/mol.cm, 231 nm), while amlodipine (5886.72-10914.96 L/mol.cm, 215 nm), (5518.8-6418.16 L/mol.cm, 254 nm) and (1676.08-1921.36 L/mol.cm, 277 nm). The recovery rate of lisinopril dihydrate in the pharmaceutical dosage forms range was 95.13 to 102.60% and amlodipine 95.14 to 102.80%. The results of the relative error showed that the interferences did not affect the method of estimating these compounds. The proposed method has been successfully applied to estimate pharmaceutical dosage forms.
GC/EI-MS and UV-Vis analysis of pesticide residues in cultivated Catha edulis Forsk (Khat) from selected farms in Meru County, Kenya
Authors: Oyugi Albert Morang’a , Adongo John Onyango , Mudalungu Cynthia Muhavi , Kibet Joshua Kiprotich
Published: March 2023

In this study, an analysis of pesticide residues was performed using a gas chromatography/ electron impact mass spectrometer (GC/EI-MS) to qualitatively assess and characterize pesticide residues in khat leaves sampled from selected agricultural farms in Meru County, Kenya. A solid-phase microextraction (SPME) procedure followed by GC/EI-MS analysis led to the detection and identification of six pesticide compounds from the sample-ion chromatograms. They include cypermethrin, acephate, cyhalothrin, cyfluthrin, chlorpyrifos, and chlorfenvinphos. The prevalence rate of pesticide contamination was determined to be 54.5% of the sample size. Of the identified pesticide residues, 50% were compounds based on pyrethroids and the other 50% were based on organophosphate. Four of the six identified pesticides were chlorinated compounds. A quick, easy, cheap, effective, rugged, and safe UV-vis double beam spectrophotometric technique based on copper (II) chelation reactions leading to colored copper pesticide complexes was developed, validated, and applied to quantify and compare the levels of selected pesticide compounds found in the khat samples. UV-vis wavelength-scan measurements performed on pesticide compounds chelated with copper (II) ions revealed maximum absorption of Cu-cypermethrin and Cu-acephate at 321 and 207 nm, respectively. The standards calibration curves developed from the UV-Vis quantitation technique showed excellent linearity in the concentration range of 0.5-10.0 µg/L (R2 = 0.99) for both cypermethrin and acephate standards. The estimated limits of quantification (LOQ) were 0.25-0.26 µg/L, respectively. The UV-Vis quantitation results from the selected samples (in which residues were confirmed to be present) revealed that acephate (an organophosphate residue) occurred at higher concentration levels (range 2.897-7.978 µg/L) than cypermethrin (2.145 µg/L). For the pesticides quantitatively analysed in the selected samples, the levels were below the maximum residue limit (MRL). The hazard quotients (HQ) were in the range of between 0.247-0.797.