bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Assessment of Collagen and Its Crosslink Content in the Humerus from Primiparous Dairy Cows with Spontaneous Humeral Fractures Due to Osteoporosis from New Zealand
Authors: Wehrle-Martinez Alvaro , Naffa Rafea , Back Penny , Rogers Chris W. , Lawrence Kevin , Loo Trevor , Sutherland-Smith Andrew , Dittmer Keren
Published: September 2022

Numerous cases of spontaneous humeral fracture in primiparous dairy cows from New Zealand have prompted the study of the condition to establish probable causes or risk factors associated with the condition. Previous studies identified inadequate protein-calorie malnutrition as an important contributory factor. Earlier case studies also reported that ~50% of cows have low liver and/or serum copper concentration at the time of humeral fracture. Because copper is so closely associated with the formation of collagen cross-links, the aim of this study was to compare collagen and collagen crosslink content in the humerus from primiparous cows with and without humeral fractures and to determine the role of copper in the occurrence of these fractures. Humeri were collected from cows with and without humeral fractures, ground, and the collagen and collagen cross-link content measured using high-performance liquid chromatography. Collagen content was significantly higher in the humeri of cows without humeral fractures, while total collagen crosslink content was significantly higher in the humerus of cows with humeral fractures. These results indicate other factor/s (e.g., protein-calorie undernutrition) might be more important than the copper status in the occurrence of humeral fractures in dairy cows in New Zealand.
From Contagium vivum fluidum to Riboviria: A Tobacco Mosaic Virus-Centric History of Virus Taxonomy
Authors: Zerbini F. Murilo , Kitajima Elliot W.
Published: September 2022

Viruses were discovered as agents of disease in the late 19th century, but it was not until the 1930s that the nature of these agents was elucidated. Nevertheless, as soon as viral diseases started to be recognized and cataloged, there were attempts to classify and name viruses. Although these early attempts failed to be adopted by the nascent virology community, they are evidence of the human compulsion to try to organize the natural world into well-defined categories. Different classification schemes were proposed during the 20th century, but again none were widely embraced by virologists. In 1966, with the creation of the International Committee on Nomenclature of Viruses (eventually renamed as the International Committee on Taxonomy of Viruses), a more organized effort led to an official taxonomy in which viruses were classified into families and genera. At present, a much better understanding of the evolutionary relationships among viruses has led to the establishment of a 15-rank taxonomy based primarily on these evolutionary relationships. This review of virus taxonomy will be centered on the tobacco mosaic virus (TMV), the agent of the disease studied by Dmitry Ivanovsky and the first virus to be recognized as such, which was often historically at the center of major advancements in virology during the 20th century.
Integration of Nanometer-Range Label-to-Label Distances and Their Distributions into Modelling Approaches
Authors: Jeschke Gunnar
Published: September 2022

Labelling techniques such as electron paramagnetic resonance spectroscopy and single-molecule fluorescence resonance energy transfer, allow access to distances in the range of tens of angstroms, corresponding to the size of proteins and small to medium-sized protein complexes. Such measurements do not require long-range ordering and are therefore applicable to systems with partial disorder. Data from spin-label-based measurements can be processed into distance distributions that provide information about the extent of such disorder. Using such information in modelling presents several challenges, including a small number of restraints, the influence of the label itself on the measured distance and distribution width, and balancing the fitting quality of the long-range restraints with the fitting quality of other restraint subsets. Starting with general considerations about integrative and hybrid structural modelling, this review provides an overview of recent approaches to these problems and identifies where further progress is needed.
Role of Ion Channels in the Development of Pulmonary Arterial Hypertension
Authors: Antigny Fabrice
Published: September 2022

Pulmonary arterial hypertension (PAH) is an uncommon, progressive, and fatal disease [...]
Radiobiological Aspects of FLASH Radiotherapy
Authors: Hageman Eline , Che Pei-Pei , Dahele Max , Slotman Ben J. , Sminia Peter
Published: September 2022

Radiotherapy (RT) is one of the primary treatment modalities for cancer patients. The clinical use of RT requires a balance to be struck between tumor effect and the risk of toxicity. Sparing normal tissue is the cornerstone of reducing toxicity. Advances in physical targeting and dose-shaping technology have helped to achieve this. FLASH RT is a promising, novel treatment technique that seeks to exploit a potential normal tissue-sparing effect of ultra-high dose rate irradiation. A significant body of in vitro and in vivo data has highlighted a decrease in acute and late radiation toxicities, while preserving the radiation effect in tumor cells. The underlying biological mechanisms of FLASH RT, however, remain unclear. Three main mechanisms have been hypothesized to account for this differential FLASH RT effect between the tumor and healthy tissue: the oxygen depletion, the DNA damage, and the immune-mediated hypothesis. These hypotheses and molecular mechanisms have been evaluated both in vitro and in vivo. Furthermore, the effect of ultra-high dose rate radiation with extremely short delivery times on the dynamic tumor microenvironment involving circulating blood cells and immune cells in humans is essentially unknown. Therefore, while there is great interest in FLASH RT as a means of targeting tumors with the promise of an increased therapeutic ratio, evidence of a generalized FLASH effect in humans and data to show that FLASH in humans is safe and at least effective against tumors as standard photon RT is currently lacking. FLASH RT needs further preclinical investigation and well-designed in-human studies before it can be introduced into clinical practice.
Mechanics and Energetics of Human Feet: A Contemporary Perspective for Understanding Mobility Impairments in Older Adults
Authors: Takahashi Kota Z. , Krupenevich Rebecca L. , Lenz Amy L. , Kelly Luke A. , Rainbow Michael J. , Franz Jason R.
Published: September 2022

Much of our current understanding of age-related declines in mobility has been aided by decades of investigations on the role of muscle–tendon units spanning major lower extremity joints (e.g., hip, knee and ankle) for powering locomotion. Yet, mechanical contributions from foot structures are often neglected. This is despite the emerging evidence of their critical importance in youthful locomotion. With the rapid growth in the field of human foot biomechanics over the last decade, our theoretical knowledge of young asymptomatic feet has transformed, from long-held views of the foot as a stiff lever and a shock absorber to that of a versatile system that can modulate mechanical power and energy output to accommodate various locomotor task demands. In this perspective review, we predict that the next set of impactful discoveries related to locomotion in older adults will emerge by integrating the novel tools and approaches that are currently transforming the field of human foot biomechanics. By illuminating the functions of the feet in older adults, we envision that future investigations will refine our mechanistic understanding of mobility deficits affecting our aging population, which may ultimately inspire targeted interventions to rejuvenate the mechanics and energetics of locomotion.
Cytokine Profile at Diagnosis Affecting Trough Concentration of Infliximab in Pediatric Crohn’s Disease
Authors: Kwon Yiyoung , Kim Eun-Sil , Kim Yoon-Zi , Choe Yon-Ho , Kim Mi-Jin
Published: September 2022

Background: This study aims to measure the concentration of cytokines produced during the inflammation process to investigate if there are any differences in response to treatment of pediatric Crohn’s disease and to determine if the initial tumor necrosis factor-alpha (TNF-α) level affected the trough concentration of infliximab (IFX). Methods: This study included 30 pediatric patients with moderate-to-severe Crohn’s disease. At the time of diagnosis, blood samples were collected for the measurement of cytokines (IL-6, TNF-α, IL-17A, and IL-10). Blood samples were extracted from patients who had begun IFX treatment to measure the IFX trough concentration immediately before the fourth dose administration. Results: All cytokines (TNF-α, IL-6, IL-10, and IL-17A) were significantly higher in patients who did not achieve clinical or biochemical remission than in those who did (p = 0.027, 0.006, 0.017, 0.032, respectively). TNF-α had a negative correlation with the IFX trough concentration (Pearson coefficient = −0.425, p = 0.034). The diagnostic capability of the initial TNF-α concentration to predict under the therapeutic IFX trough concentration, defined as less than 3 µg/mL, had an area under the receiver operating characteristic of 0.730 (p = 0.049). The TNF-α concentration was set at 27.6 pg/mL as the cutoff value. Conclusions: Measuring cytokines at the time of diagnosis can be used to predict the treatment response. Measuring the initial TNF-α concentration may help to predict the treatment response to IFX. When the initial TNF-α concentration is greater than 27.6 pg/mL, a higher dose of IFX may be more appropriate than routinely administering 5 mg/kg of IFX to maintain the therapeutic concentration.
Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein
Authors: Chang Ting-Chou , Sun Aileen Y. , Huang Yu-Chung , Wang Chih-Hui , Wang Shau-Chun , Chau Lai-Kwan
Published: September 2022

The global pandemic of COVID-19 has created an unrivalled need for sensitive and rapid point-of-care testing (POCT) methods for the detection of infectious viruses. For the novel coronavirus SARS-CoV-2, the nucleocapsid protein (N-protein) is one of the most abundant structural proteins of the virus and it serves as a useful diagnostic marker for detection. Herein, we report a fiber optic particle plasmon resonance (FOPPR) biosensor which employed a single-stranded DNA (ssDNA) aptamer as the recognition element to detect the SARS-CoV-2 N-protein in 15 min with a limit of detection (LOD) of 2.8 nM, meeting the acceptable LOD of 106 copies/mL set by the WHO target product profile. The sensor chip is a microfluidic chip based on the balance between the gravitational potential and the capillary force to control fluid loading, thus enabling the power-free auto-flowing function. It also has a risk-free self-contained design to avoid the risk of the virus leaking into the environment. These findings demonstrate the potential for designing a low-cost and robust POCT device towards rapid antigen detection for early screening of SARS-CoV-2 and its related mutants.
Cervical Imaging in the Low Resource Setting: A Review
Authors: Gonzalez Mariacarla , Boonya-Ananta Tananant , Madhivanan Purnima , Ramella-Roman Jessica C.
Published: September 2022

Cervical cancer is one of the most significant global health inequities of our time and is the fourth most common cancer in women worldwide, disproportionally affecting developing countries where the disease burden is 84%. Sometimes referred to as preventable cancer, it progresses slowly, providing a window of time for routine screening in which pre-cancerous lesions can be identified and treated. The high mortality rate can be attributed to many reasons, including the high cost of cytology-based screening, lack of human resources to conduct screening, and inadequate preventive medicine services and systems. Due to its slow progression, early intervention is feasible with appropriate screening. However, the standard screening procedures require access to lab-based tests and physician expertise. Several imaging devices have been introduced in the literature to aid cervical screening in low-resource settings. This review details the instrumentation and clinical testing of devices currently deployed in low-resource locations worldwide. The devices’ imaging, portability, illumination, and power requirements (among other metrics) are documented with specifics of human pilot studies conducted with these tools.
Biosensors and Drug Delivery in Oncotheranostics Using Inorganic Synthetic and Biogenic Magnetic Nanoparticles
Authors: Zimina Tatiana M. , Sitkov Nikita O. , Gareev Kamil G. , Fedorov Viacheslav , Grouzdev Denis , Koziaeva Veronika , Gao Huile , Combs Stephanie E. , Shevtsov Maxim
Published: September 2022

Magnetic nanocarriers have attracted attention in translational oncology due to their ability to be employed both for tumor diagnostics and therapy. This review summarizes data on applications of synthetic and biogenic magnetic nanoparticles (MNPs) in oncological theranostics and related areas. The basics of both types of MNPs including synthesis approaches, structure, and physicochemical properties are discussed. The properties of synthetic MNPs and biogenic MNPs are compared with regard to their antitumor therapeutic efficiency, diagnostic potential, biocompatibility, and cellular toxicity. The comparative analysis demonstrates that both synthetic and biogenic MNPs could be efficiently used for cancer theranostics, including biosensorics and drug delivery. At the same time, reduced toxicity of biogenic particles was noted, which makes them advantageous for in vivo applications, such as drug delivery, or MRI imaging of tumors. Adaptability to surface modification based on natural biochemical processes is also noted, as well as good compatibility with tumor cells and proliferation in them. Advances in the bionanotechnology field should lead to the implementation of MNPs in clinical trials.
Reversible Splenial Lesion Syndrome (RESLES) after Nitrous Oxide Abuse: A Case Report
Authors: Tao Yiming , Han Jie , Jian Xiangdong , Li Yongsheng
Published: September 2022

Background: Reversible splenial-lesion syndrome (RESLES) is a relatively rare and underrecognized clinical-imaging syndrome involving the splenium of the corpus callosum (SCC). RESLES can be caused by various etiologies. Case description: An 18-year-old man with no previous history of neurological or psychiatric disorders presented to our hospital with headache, intermittent blurred vision, and limb weakness after 150 days of recreational nitrous-oxide abuse. The patient’s serum vitamin B12 concentration was normal, and magnetic-resonance imaging (MRI) examination revealed isointensity on T1-weighted imaging (T1WI) of the corpus callosum and high signal intensity on T2WI, T2FLAIR, and diffusion-weighted MRI (DWI); thus, a diagnosis of RESLES was established. The patient received 0.5 mg of mecobalamin daily and nitrous oxide was discontinued. After 4 weeks, the patient’s symptoms disappeared and the imaging examination revealed normal findings. Conclusion: We report for the first time a case of headache, blurred vision, and hallucination caused by RESLES associated with nitrous-oxide abuse. In cases of headaches and hallucinations of unknown etiology, the possibility of RESLES caused by nitrous oxide abuse should be considered.
Pimavanserin and Parkinson’s Disease Psychosis: A Narrative Review
Authors: Rissardo Jamir Pitton , Durante Ícaro , Sharon Idan , Fornari Caprara Ana Letícia
Published: September 2022

Pimavanserin (PMV) is the first approved drug for treating hallucinations and delusions in Parkinson’s disease (PD) psychosis. Psychosis is one of the leading causes of nursing home placement in people with PD. Furthermore, hallucinations are a more frequent cause of institutionalization than motor disability or dementia related to PD. The management of PD psychosis involves antipsychotic medications. Most of the drugs in this class directly block dopamine D2 receptors, leading to significantly worsening motor symptoms in patients with PD. The most commonly used medications for managing PD psychosis are quetiapine, clozapine, and PMV. This literature review aims to study pimavanserin’s history, mechanism, clinical trials, and post-marketing experience. PMV is a potent 5-HT2A receptor antagonist/inverse agonist. Moreover, this drug can interact with 5-HT2C receptors. We calculated some physicochemical descriptors and pharmacokinetic properties of PMV. Eight clinical trials of PMV and PD psychosis are registered on ClinicalTrials.gov. Only four of them have complete results already published. Meta-analytic results showed that PMV efficacy is inferior to clozapine. However, PMV has a significantly lower number of side-effects for managing psychosis in PD. Medicare database assessment revealed 35% lower mortality with PMV compared to other atypical antipsychotics. Moreover, sensitive statistical analysis demonstrated that PMV is a protective factor for the risk of falls in individuals with PD.
Assessment of Executive and Cognitive Functions in Children with Restless Sleep Disorder: A Pilot Study
Authors: DelRosso Lourdes M. , Vega-Flores German , Ferri Raffaele , Mogavero Maria P. , Diamond Adele
Published: September 2022

Restless sleep disorder affects children and is characterized by frequent nocturnal movements, iron deficiency, and daytime symptoms such as poor school performance or behavioral problems. Although sleep parameters have been thoroughly studied and daytime sleepiness has been previously assessed, neurocognitive and executive functions have not. In this study, we evaluated neurocognitive functions in a group of 13 children diagnosed with restless sleep disorder using the National Institute of Health Toolbox (NIH toolbox). The mean age was 10.62 (S.D. 2.785). Among them, seven were male and six were female. The fully corrected T-scores (adjusted for demographic variables: age, ethnicity, and education level) showed the lowest values for the Flanker test (selective attention) and dimensional change card sorting test (cognitive flexibility and inhibitory control), with a very large effect size vs. the corresponding expected frequencies. For all the other tests, the average scores were 50; however, individual children scored low on pattern recognition and two composite scores (fluid and total). In conclusion, these data support the fact that cognitive functions are affected in children with restless sleep disorder, especially selective attention. Clinicians must recognize sleep disorders and daytime impairment in order to promptly intervene and prevent cognitive impairments.
Understanding Disclosure of Health Information to Workplace Friends
Authors: Kingsley Westerman Catherine Y. , Haverkamp Emily M. , Zeng Cheng
Published: September 2022

The purpose of this study was to learn about the process of disclosing health information to a coworker friend using the lens of Communication Privacy Management Theory. The study explores emerging themes regarding health information disclosure and predicts associations between privacy, social support, risk, stigma, and the willingness to disclose health information to a friend at work. Employees were asked to recall a time they shared health information with a coworker friend and report about the interaction via open-ended items and scales on a survey. The study found that as emotional support, instrumental support, perceived risk, and stigma of the information increased, so did the tendency to disclose to a coworker friend. Increased privacy of the information was associated with a decrease in the tendency to disclose. A thematic analysis of the open-ended results also revealed that employees shared information associated with personal on-going health problems to seek support, to relate to their coworker friends, and to maintain their friendship. The findings also indicated that employees were likely to receive social support from their coworker friends even if they were not seeking it.
The Search for Solar Architecture in Asia in the Works of the Architect Antonin Raymond: A Protracted Balance between Culture and Nature
Authors: Almodóvar-Melendo Jose-Manuel , Rodríguez-Cunill Inmaculada , Cabeza-Lainez Joseph
Published: September 2022

The renowned architect Antonin Raymond undertook a large amount of work in Japan for more than fifty years. He arrived to the archipelago as a partner to Frank Lloyd Wright, who had received the commission for the paramount Imperial Hotel. Nevertheless, Raymond became almost revolutionary in that country for his realizations of projects and buildings. Simultaneously, he pioneered the concept of solar houses, a notion that proved to be fundamental for bioclimatic design. Raymond realized that because of the influence of geomancy and Daoism, the buildings of Japan had a sort of cosmic link, especially to the sun and the seasons, and in this way, he took great concern with solar exposure, shading, and daylighting, both in the way in which they were composed and also in the materials that were employed, such as lumber, reed roofs, and paper screens. We have tried to identify these trends in his most significant buildings and establish a sequence of how they evolved towards a contemporary and truly sustainable design. The main result presented is that for the reasons presented, Raymond influenced a number of celebrated architects in Japan, India, and the USA. In fact, instead of pursuing the pure abstract minimalism common in his epoch, he made use of the ideas of naturalism in architecture, which in the case of Asia were related to ritual cosmology. In this article, we have outlined how such a procedure was possible and what it entailed for complex domains such as sun-lighting and daylighting, ventilation, acoustics, and re-interpretation of vernacular architecture. The connection with the main forces of the environment, which effectively interact through architecture, when considered from a philosophical and even spiritual point of view as he did, resulted in a kind of artistic revitalization of traditions belonging to Daoism and Shintoism and inaugurated a new manner of sustainable thinking for the building profession that endures today.
Smart Tech 4.0 in the Built Environment: Applications of Disruptive Digital Technologies in Smart Cities, Construction, and Real Estate
Authors: Ullah Fahim
Published: September 2022

Since the beginning of industrialization, there have been several paradigm shifts initiated through technological revolutions, inventions, and leaps [...]
Knowledge Mapping Analysis of the Study of Rural Landscape Ecosystem Services
Authors: Wang Yinyi , Zhang Yaping , Yang Guofu , Cheng Xiaomeng , Wang Jing , Xu Bin
Published: September 2022

Understanding the research lineage of rural landscape ecosystem services (RLESs) is of importance for improving rural landscapes and developing sustainable ecosystem services. However, there is currently no literature analysis on the scientific quantification and visualization of RLESs. In this study, 4524 articles related to RLESs from 1990 to 2021 were analyzed using the bibliometric method and ISI Web of Science database. The results show that RLES research hotspots have gradually shifted from the early keywords of “vegetation”, “land use change”, “agriculture”, “rural gradients” and “models” to the emerging “cultural ecosystem services”, “rural tourism”, “landscape preferences” and “policy guidance”. Scholars from developed and developing countries place different emphases on research hotspots in terms of research content, scale and methodology due to differences in their research backgrounds and other aspects. In addition, five categories of research fronts were obtained through literature co-citation analysis. Through burst word detection analysis, combined with basic research and research hotspots and frontier analysis, we concluded that future RLES research will focus on four areas: (1) the relationship and collaboration between and management of biodiversity and ecosystem services; (2) the landscape value of RLESs; (3) land-use changes and ecosystem service values; and (4) research methods for innovative RLESs. Our findings may contribute to better in-depth RLES research by providing a theoretical reference and practical help for future related research.
Condition Prediction for Existing Educational Facilities Using Artificial Neural Networks and Regression Analysis
Authors: Hassan Ahmed M. , Adel Kareem , Elhakeem Ahmed , Elmasry Mohamed I. S.
Published: September 2022

Infrastructural assets such as roads, bridges, and buildings make a considerable contribution to national economies. These assets deteriorate due to aging, environmental conditions, and other external factors. Maintaining the performance of an asset in line with rational repair strategies represents a considerable challenge for decision-makers, who may not pay attention to developing adequate maintenance plans or leave the assets unmaintained. Worldwide, organizations are under pressure to ensure the sustainability of their assets. Such organizations may burden their treasury with random maintenance operations, especially with a limited budget. This research aims to develop a generalized condition assessment approach to monitor and evaluate existing facility elements. The proposed approach represents a methodology to determine the element condition index (CI). The methodology is reinforced with an artificial neural network (ANN) model to predict the element deterioration. The performance of this model was evaluated by comparing the obtained predicted CIs with ordinary least squares (OLS) regression model results to choose the most accurate prediction technique. A case study was applied to a group of wooden doors. The ANN model showed reliable results with R2 values of 0.99, 0.98, and 0.99 for training, cross-validation, and testing sets, respectively. In contrast, the OLS model R2 value was 1.00. These results show the high prediction capability of both models with an advantage to the OLS model. Applying this approach to different elements can help decision-makers develop a preventive maintenance schedule and provide the necessary funds.
Experimental and Seismic Response Study of Laminated Rubber Bearings Considering Different Friction Interfaces
Authors: Zhang Bingzhe , Wang Kehai , Lu Guanya , Qiu Wenhua , Yin Weitao
Published: September 2022

Unbonded LRBs (laminated rubber bearings) are commonly applied in small-to-medium-span bridges in China. The frictional sliding characteristics of LRBs have a vital influence on the seismic response of the bridge. Nine square LRBs were subjected to the quasi-static displacement loading test in this paper, and the differences in sliding characteristics of LRBs at the interface of steel and concrete test pad were investigated. The variation of the friction coefficient during sliding was then analyzed. Based on the experimental data, a three-fold mechanical constitutive model of LRBs that considers the breakaway-sliding friction characteristics is established. Further, the bridge seismic demands in longitudinal directions with different friction interfaces are compared by nonlinear dynamic analysis on a typical LRB-supported concrete bridge. The results show the causalities of the displacements and decreases of the friction coefficient of the LRB. The breakaway coefficient of friction of the concrete surface was generally greater than that of the steel in the pre-sliding stage, while the sliding coefficient of friction of the steel interface in the post-sliding stage was greater than that of the concrete. Moreover, the proposed three-fold constitutive model is able to simulate the frictional sliding behavior of LRBs accurately. Lastly, the seismic design of small-to-medium-span bridges should take into account the breakaway-sliding friction effect of the LRBs and the preference for steel as friction pads for LRBs is recommended.
Augmented and Virtual Reality (AR/VR) for Education and Training in the AEC Industry: A Systematic Review of Research and Applications
Authors: Tan Yi , Xu Wenyu , Li Shenghan , Chen Keyu
Published: September 2022

With updated equipment and maturing technology, the applications of augmented and virtual reality (AR/VR) technologies in the architecture, engineering, and construction (AEC) industry are receiving increasing attention rapidly. Especially in education and training, an increasing number of researchers have started to implement AR/VR technologies to provide students or trainees with a visual, immersive, and interactive environment. In this article, a systematic review of AR/VR technologies for education and training in the AEC industry is conducted. First of all, through comprehensive analysis, 82 related studies are identified from two databases, namely Scopus and Web of Science. Secondly, the VOSviewer is used to analyze the current status of AR/VR for education and training in the AEC industry. Thirdly, the identified studies are classified into different categories according to their application domains by qualitative analysis. Fourthly, after a further filtering, 17 out of the 82 studies are included in the meta-analysis to quantify the actual impact of AR/VR. The results indicate that there are some limitations in the applications of AR/VR for education and training in the AEC industry. Finally, to further explore the reasons for the existence of limitations, the 82 studies are summarized to analyze the current challenges of AR/VR for education and training in the AEC industry. This study also provides insights into future trends in AR/VR for education and training in the AEC industry.
Development and In Vitro Assessment of a Novel Vacuum-Based Tissue-Holding Device for Laparoscopic and Robotic Kidney Cancer Operations
Authors: Gabi Michael , Bieri Uwe , Ramakrishnan Venkat , Niemann Tilo , Nocito Antonio , Brader Nadine , Maake Caroline , Hefermehl Lukas John
Published: September 2022

In this paper, we describe the development and evaluation of a novel tissue-holding device (THD) for use during robotic-assisted laparoscopic partial nephrectomy. The THD is a vacuum-based apparatus made of either 3D-printed polyethylene or stainless steel. The proximal end connects to suction tubing routed outside the body, while the distal end is conically shaped and designed to firmly interface with the tumor. Device feasibility studies were performed on six porcine kidneys, two porcine livers, and two embalmed human cadavers. A Likert-scale rating was used to assess device setup, suction, and tissue handling. Additional tests were performed using the daVinci Xi® robotic system. Finally, the holding force of the THD was assessed using different standard vacuum systems and pressure settings. In porcine tissue, the device setup, tissue suction, and handling were rated as “good”. THD insertion and removal was uncomplicated. In a simulated transabdominal approach on fixed human cadavers, the device setup, suction, and tissue handling were also rated as “good”. No macroscopic tissue compromise or device deterioration was noted. The handling and holding abilities using the daVinci Xi® robotic system were also rated “good”. The device was able to successfully hold over 300 g of tissue at a suction pressure of −600 mmHg. The preliminary evaluation of the THD demonstrated satisfactory results.
Breast-Specific Gamma Imaging: An Added Value in the Diagnosis of Breast Cancer, a Systematic Review
Authors: De Feo Maria Silvia , Sidrak Marko Magdi Abdou , Conte Miriam , Frantellizzi Viviana , Marongiu Andrea , De Cristofaro Flaminia , Nuvoli Susanna , Spanu Angela , De Vincentis Giuseppe
Published: September 2022

Purpose: Breast cancer is the most common solid tumor and the second highest cause of death in the United States. Detection and diagnosis of breast tumors includes various imaging modalities, such as mammography (MMG), ultrasound (US), and contrast-enhancement MRI. Breast-specific gamma imaging (BSGI) is an emerging tool, whereas morphological imaging has the disadvantage of a higher absorbed dose. Our aim was to assess if this imaging method is a more valuable choice in detecting breast malignant lesions compared to morphological counterparts. Methods: research on Medline from 1995 to June 2022 was conducted. Studies that compared at least one anatomical imaging modality with BSGI were screened and assessed through QUADAS2 for risk of bias and applicability concerns assessment. Sensitivity, specificity, positive and negative predictive value (PPV and NPV) were reported. Results: A total of 15 studies compared BSGI with MMG, US, and MRI. BSGI sensitivity was similar to MRI, but specificity was higher. Specificity was always higher than MMG and US. BSGI had higher PPV and NPV. When used for the evaluation of a suspected breast lesion, the overall sensitivity was better than the examined overall sensitivity when BSGI was excluded. Risk of bias and applicability concerns domain showed mainly low risk of bias. Conclusion: BSGI is a valuable imaging modality with similar sensitivity to MRI but higher specificity, although at the cost of higher radiation burden.
Communication Tools Used in Cancer Communication with Children: A Scoping Review
Authors: Yamaji Noyuri , Suzuki Daichi , Suto Maiko , Sasayama Kiriko , Ota Erika
Published: September 2022

Background: Although communication tools might guide healthcare professionals in communicating with children about cancer, it is unclear what kind of tools are used. This scoping review aimed to map the communication tools used in cancer communication among children with cancer, families, and healthcare professionals. Methods: A comprehensive search using PubMed (including MEDLINE), Embase, CENTRAL, PsycINFO, and CINAHL was conducted on 1 August 2021. We mapped communication tools and their impacts. Results: We included 25 studies (9 experimental studies and 16 feasibility studies) of 29 reports and found 21 communication tools. There was a lack of communication tools that were (1) accessible and validated, (2) designed for healthcare professionals, (3) targeted children, families, and healthcare professionals, and (4) were designed to meet the needs of children and families. Experimental studies showed that the communication tools improved children’s knowledge and psychological outcomes (e.g., health locus of control, quality of life, self-efficacy). Conclusion: We mapped communication tools and identified areas that needed further research, including a lack of tools to guide healthcare professionals and share information with children and families. Further research is needed to develop and evaluate these communication tools. Moreover, it is necessary to investigate how communication tools support children, families, and healthcare professionals.
MAPK Signaling Pathway in Oral Squamous Cell Carcinoma: Biological Function and Targeted Therapy
Authors: Cheng Yuxi , Chen Juan , Shi Yuxin , Fang Xiaodan , Tang Zhangui
Published: September 2022

Oral squamous cell carcinoma accounts for 95% of human head and neck squamous cell carcinoma cases. It is highly malignant and aggressive, with a poor prognosis and a 5-year survival rate of <50%. In recent years, basic and clinical studies have been performed on the role of the mitogen-activated protein kinase (MAPK) signaling pathway in oral cancer. The MAPK signaling pathway is activated in over 50% of human oral cancer cases. Herein, we review research progress on the MAPK signaling pathway and its potential therapeutic mechanisms and discuss its molecular targeting to explore its potential as a therapeutic strategy for oral squamous cell carcinoma.
Cancer as a Channelopathy—Appreciation of Complimentary Pathways Provides a Different Perspective for Developing Treatments
Authors: Gould Harry J. , Paul Dennis
Published: September 2022

Life depends upon the ability of cells to evaluate and adapt to a constantly changing environment and to maintain internal stability to allow essential biochemical reactions to occur. Ions and ion channels play a crucial role in this process and are essential for survival. Alterations in the expression of the transmembrane proteins responsible for maintaining ion balance that occur as a result of mutations in the genetic code or in response to iatrogenically induced changes in the extracellular environment is a characteristic feature of oncogenesis and identifies cancer as one of a constellation of diseases known as channelopathies. The classification of cancer as a channelopathy provides a different perspective for viewing the disease. Potentially, it may expand opportunities for developing novel ways to affect or reverse the deleterious changes that underlie establishing and sustaining disease and developing tolerance to therapeutic attempts at treatment. The role of ions and ion channels and their interactions in the cell’s ability to maintain ionic balance, homeostasis, and survival are reviewed and possible approaches that mitigate gain or loss of ion channel function to contribute to new or enhance existing cancer therapies are discussed.
Advances in Image-Guided Radiotherapy in the Treatment of Oral Cavity Cancer
Authors: Nien Hsin-Hua , Wang Li-Ying , Liao Li-Jen , Lin Ping-Yi , Wu Chia-Yun , Shueng Pei-Wei , Chung Chen-Shuan , Lo Wu-Chia , Lin Shih-Chiang , Hsieh Chen-Hsi
Published: September 2022

Image-guided radiotherapy (IGRT) is an advanced auxiliary radiotherapy technique. During cancer treatment, patients with oral cavity cancer (OCC) experience not only disease but also adverse effects due to RT. IGRT provides the relevant advantages of RT by precisely delivering tumoricidal doses via real-time knowledge of the target volume location and achieves maximal tumor control with minimal complications as recommended for cancer treatment. Additionally, studies have shown that IGRT can improve clinical outcomes in terms of not only treatment side effects but also survival benefits for cancer patients. IGRT can be performed alongside various imaging methods, including computed tomography and magnetic resonance imaging, and at different times during the radiotherapy regimen. This article reviews the literature to discuss the effects and importance of IGRT for patients with OCC, examines the rationale underlying the advantages of IGRT, discusses the limitations of IGRT with respect to different techniques, and summarizes the strategies and future prospects of IGRT in the treatment of OCC.
Non-Invasive Biomarkers for Immunotherapy in Patients with Hepatocellular Carcinoma: Current Knowledge and Future Perspectives
Authors: Pallozzi Maria , Di Tommaso Natalia , Maccauro Valeria , Santopaolo Francesco , Gasbarrini Antonio , Ponziani Francesca Romana , Pompili Maurizio
Published: September 2022

The treatment perspectives of advanced hepatocellular carcinoma (HCC) have deeply changed after the introduction of immunotherapy. The results in responders show improved survival compared with Sorafenib, but only one-third of patients achieve a significant benefit from treatment. As the tumor microenvironment exerts a central role in shaping the response to immunotherapy, the future goal of HCC treatment should be to identify a proxy of the hepatic tissue condition that is easy to use in clinical practice. Therefore, the search for biomarkers that are accurate in predicting prognosis will be the hot topic in the therapeutic management of HCC in the near future. Understanding the mechanisms of resistance to immunotherapy may expand the patient population that will benefit from it, and help researchers to find new combination regimens to improve patients’ outcomes. In this review, we describe the current knowledge on the prognostic non-invasive biomarkers related to treatment with immune checkpoint inhibitors, focusing on serological markers and gut microbiota.
Clinical and Prognostic Significance of the Eighth Edition Oral Cancer Staging System
Authors: Ghantous Yasmin , Nashef Aysar , Sidransky David , Abdelraziq Murad , Alkeesh Kutaiba , Araidy Shareef , Koch Wayne , Brait Mariana , Abu El-Naaj Imad
Published: September 2022

Objectives: The most notable changes in the eighth edition of the AJCC Cancer Staging System include incorporating the depth of invasion (DOI) into T staging and extranodal extension (ENE) into N staging. In this study, we retrospectively assessed the prognostic and clinical implications of the eighth TNM staging system. Materials and Methods: Patients with Oral Squamous Cell Carcinoma (OSCC) who were treated surgically between 2010 and 2017 were retrospectively reviewed. Tumors were first staged according to the seventh edition and restaged using the eighth edition. The prognostic value of the resultant upstaging was evaluated. Results: Integrating the DOI into the T classification resulted in the upstaging of 65 patients, whereas incorporating ENE into the N staging resulted in the upstaging of 18 patients (p < 0.001). Upstaging due to DOI integration had no significant effect on OS or DSS (p > 0.05). Conclusion: Our results demonstrate the importance of incorporating ENE into nodal staging and considering adjuvant therapy when ENE is present.
Bone Mineral Density as an Individual Prognostic Biomarker in Patients with Surgically-Treated Brain Metastasis from Lung Cancer (NSCLC)
Authors: Ilic Inja , Potthoff Anna-Laura , Borger Valeri , Heimann Muriel , Paech Daniel , Giordano Frank Anton , Schmeel Leonard Christopher , Radbruch Alexander , Schuss Patrick , Schäfer Niklas , Herrlinger Ulrich , Vatter Hartmut , Lakghomi Asadeh , Schneider Matthias
Published: September 2022

Patients with BM are in advanced stages of systemic cancer, which may translate into significant alterations of body composition biomarkers, such as BMD. The present study investigated the prognostic value of BMD on overall survival (OS) of 95 patients with surgically-treated BM related to NSCLC. All patients were treated in a large tertiary care neuro-oncological center between 2013 and 2018. Preoperative BMD was determined from the first lumbar vertebrae (L1) from routine preoperative staging computed tomography (CT) scans. Results were stratified into pathologic and physiologic values according to recently published normative reference ranges and correlated with survival parameters. Median preoperative L1-BMD was 99 Hounsfield units (HU) (IQR 74–195) compared to 140 HU (IQR 113–159) for patients with pathological and physiologic BMD (p = 0.03), with a median OS of 6 versus 15 months (p = 0.002). Multivariable analysis revealed pathologic BMD as an independent prognostic predictor for increased 1-year mortality (p = 0.03, OR 0.5, 95% CI 0.2–1.0). The present study suggests that decreased preoperative BMD values may represent a previously unrecognized negative prognostic factor in patients of BM requiring surgery for NSCLC. Based on guideline-adherent preoperative staging, BMD may prove to be a highly individualized, readily available biomarker for prognostic assessment and treatment guidance in affected patients.
Lin28 Regulates Cancer Cell Stemness for Tumour Progression
Authors: Lin Zhuohui , Radaeva Mariia , Cherkasov Artem , Dong Xuesen
Published: September 2022

Tumours develop therapy resistance through complex mechanisms, one of which is that cancer stem cell (CSC) populations within the tumours present self-renewable capability and phenotypical plasticity to endure therapy-induced stress conditions and allow tumour progression to the therapy-resistant state. Developing therapeutic strategies to cope with CSCs requires a thorough understanding of the critical drivers and molecular mechanisms underlying the aforementioned processes. One such hub regulator of stemness is Lin28, an RNA-binding protein. Lin28 blocks the synthesis of let-7, a tumour-suppressor microRNA, and acts as a global regulator of cell differentiation and proliferation. Lin28also targets messenger RNAs and regulates protein translation. In this review, we explain the role of the Lin28/let-7 axis in establishing stemness, epithelial-to-mesenchymal transition, and glucose metabolism reprogramming. We also highlight the role of Lin28 in therapy-resistant prostate cancer progression and discuss the emergence of Lin28-targeted therapeutics and screening methods.
The Crosstalk between Microbiome and Immunotherapeutics: Myth or Reality
Authors: Tojjari Alireza , Abushukair Hassan , Saeed Anwaar
Published: September 2022

The gut microbiome refers to microorganisms and their genetic material influencing local and systemic inflammation. Inflammation is known to contribute to cancer development, progression, and treatment. Evidence suggests that modulating the gut microbiome may affect responses to various cancer therapies. The gut microbiota has been suggested to have an impact on immunotherapy efficacy, especially the currently widely used immune checkpoint inhibitors in various malignancies. Microbial interventions like fecal microbiota transplantation, various probiotics, or even antibiotics can increase or decrease the tumor’s sensitivity to immunotherapy. However, not all tumors react in the same manner, highlighting the tumor microenvironment heterogeneity across tumor types and the influence this has on the crosstalk between the microbiome and therapy outcomes. In this study, we intend to review the association between the gut microbiota and immunotherapy response in cancer patients and the factors regulating this interaction.
Taraxasterol Inhibits Tumor Growth by Inducing Apoptosis and Modulating the Tumor Microenvironment in Non-Small Cell Lung Cancer
Authors: Lu Junjie , Shuai Bo , Shou Zhexing , Guo Weina , Zhou Cong , Ouyang Xiaohu , Zhou Haifeng , Li Junyi , Cui Jing , Jiang Feng , Jin Kim Yun , Sarapultsev Alexey , Li Fangfei , Zhang Ge , Luo Shanshan , Hu Desheng
Published: September 2022

Taraxasterol (TAX), one of the active components in Dandelion, demonstrated strong antitumor properties in several cancers. However, the effect and underlying mechanism of TAX in non-small cell lung cancer (NSCLC) is unclear. In this study, we showed that TAX inhibited the proliferation of cells by inducing S-phase cell cycle arrest and prevented cell migration by interfering epithelial-mesenchymal transition (EMT) in Lewis lung cancer (LLC) cells and lung carcinoma SPC-A1 cells. The pharmacological network analysis predicted that induction of apoptosis might be the potential mechanism of TAX-mediated cell deaths. Further in vitro experiments showed that TAX could significantly induce cancer cell apoptosis as verified by increased pro-apoptotic molecules including Bax, caspase-9, and PARP1 downregulated anti-apoptotic protein Bcl-2; and decreased mitochondrial potential. The LLC subcutaneous tumor model demonstrated that TAX inhibited tumor growth by induction of apoptosis and inhibition of proliferation in vivo, which is consistent with the in vitro data. Importantly, TAX administration downregulated the proportion of Treg cells and upregulated CD107a+ NK cells in the tumor microenvironment in the tumor model. Together, these data reveal that TAX performs its antitumor effect by inducing apoptosis and modulating the tumor microenvironment, providing evidence that TAX could serve as a potential natural drug for lung cancer therapy.