bluuebunny/crossref_metadata_2025 huggingface dataset:
Legal Aesthetics in The Touching Contract: Memory, Exposure and Transformation
Authors: Enright Máiréad , Kinsella Tina
Published: January 2021

This paper considers how an artwork might play a role in the political, legal and aesthetical ‘working-through’ of historical injustices. With specific reference to Sarah Browne and Jesse Jones’ participatory performance project, The Touching Contract (2016), we consider how traumatic affects from Ireland’s past continue to shape the present. Drawing primarily on the work of Jacques Rancière and Bracha L. Ettinger, we argue that The Touching Contract has the capacity to transform participants’ relation to legal history in two ways. First, by juxtaposing unspoken histories of female bodily vulnerability in relation to law with legal form during an encounter with participatory performance, this art project generates ‘mystery’, producing a productive ‘interval’ (Rancière) between the common sense of legal history and its potential re-calibration. Second, in its production of a relational ‘affectosphere’ (Kinsella), The Touching Contract provokes participants’ capacity to engage with that interval, to be genuinely affected by the transmission of legal history and respond to it in new ways.
Current perceptions of strength and conditioning coaches use of sled tow training
Authors: Williams Jason , Baghurst Timothy , Cahill Micheál J
Published: January 2021

The purpose of this study was to assess current perceptions of strength and conditioning coaches’ use of sled towing (ST) as part of their training programs. One-hundred and twenty-five coaches responded to a survey of their ST practices. Themes investigated included the primary purpose and usefulness of using ST, the loads used in short and long distances, rest times between sprints, total volume of ST sprints per session, frequency of ST activity each month, and whether coaches engaged in force-velocity profiling in ST sprints. Eighty percent of coaches either agreed or strongly agreed that ST is a useful intervention tool for improving athletic performance. Speed strength was the physiological adaptation most sought after to improve ( n = 75) followed by power ( n = 72). Bodyweight (BW) loads of 20% were the most common across all distances. The two most common rest times given between each ST repetition were one to two minutes ( n = 37) and two to three minutes ( n = 37). The most common volume responses for individual training sessions were five to eight sprints ( n = 52) and three to five times per month, respectively. These data suggest strength and conditioning coaches view ST as an integral part of programming, primarily use loads of 20% BW for both short and long sprints and seek to optimize a number of different physiological adaptations. The majority of coaches have a favorable view of ST (88%); however, current training parameters used by strength and conditioning coaches may be inadequate to achieve their desired adaptations.
Low-rank sparse subspace clustering with a clean dictionary
Authors: You Cong-Zhe , Shu Zhen-Qiu , Fan Hong-Hui
Published: January 2021

Low-Rank Representation (LRR) and Sparse Subspace Clustering (SSC) are considered as the hot topics of subspace clustering algorithms. SSC induces the sparsity through minimizing the [Formula: see text]-norm of the data matrix while LRR promotes a low-rank structure through minimizing the nuclear norm. In this paper, considering the problem of fitting a union of subspace to a collection of data points drawn from one more subspaces and corrupted by noise, we pose this problem as a non-convex optimization problem, where the goal is to decompose the corrupted data matrix as the sum of a clean and self-expressive dictionary plus a matrix of noise. We propose a new algorithm, named Low-Rank and Sparse Subspace Clustering with a Clean dictionary (LRS2C2), by combining SSC and LRR, as the representation is often both sparse and low-rank. The effectiveness of the proposed algorithm is demonstrated through experiments on motion segmentation and image clustering.
The structure and organization of collective memory representations
Authors: Mutlutürk Aysu , Tekcan Ali İ , Boduroglu Aysecan
Published: January 2021

Most collective memory research to date has focused on the temporal distribution of and generational differences in public event representations while not directly addressing the conceptual relationships between different event representations. This study investigates how individuals cluster representations of public events and how sociopolitical identity changes event clustering. Participants judged the similarity of different pairs of key public events and reported their voting behavior. Using multidimensional scaling (MDS), we identified that public events were distinguished based on their political and nonpolitical characteristics; political events were further clustered based on their specific attributes (e.g. power struggles or ethnicity issues). Voting behavior introduced variations into how people clustered political public events. Our findings suggest that collective memories that are formed in relation to a historical and sociopolitical background may be better understood within a network of relationships and at the level of specific cultures/groups rather than at a larger (e.g. national) level.
Early prolonged neutrophil activation in critically ill patients with sepsis
Authors: Törnblom Sanna , Nisula Sara , Vaara Suvi T , Poukkanen Meri , Andersson Sture , Pettilä Ville , Pesonen Eero
Published: January 2021

We hypothesised that plasma concentrations of biomarkers of neutrophil activation and pro-inflammatory cytokines differ according to the phase of rapidly evolving sepsis. In an observational study, we measured heparin-binding protein (HBP), myeloperoxidase (MPO), IL-6 and IL-8 in 167 sepsis patients on intensive care unit admission. We prospectively used the emergence of the first sepsis-associated organ dysfunction (OD) as a surrogate for the sepsis phase. Fifty-five patients (of 167, 33%) developed the first OD > 1 h before, 74 (44%) within ± 1 h, and 38 (23%) > 1 h after intensive care unit admission. HBP and MPO were elevated at a median of 12 h before the first OD, remained high up to 24 h, and were not associated with sepsis phase. IL-6 and IL-8 rose and declined rapidly close to OD emergence. Elevation of neutrophil activation markers HBP and MPO was an early event in the evolution of sepsis, lasting beyond the subsidence of the pro-inflammatory cytokine reaction. Thus, as sepsis biomarkers, HBP and MPO were not as prone as IL-6 and IL-8 to the effect of sample timing.
A quantitative analysis of two cementing techniques in total elbow replacement: A cadaveric study
Authors: Michielsen Maartje , Masson Maxime , van Haver Annemieke , Vanhees Matthias , van Riet Roger
Published: January 2021

One of the reasons for failure of total elbow replacement is loosening of the ulnar component. Cementing techniques are often outdated. A special small nozzle is needed for the ulnar component, when a cement gun is used. This may not always be available, or surgeons may prefer to use a syringe. We postulated that the use of a cement gun and smaller nozzle would result in improved filling of the ulnar canal. A cadaveric study was performed in which the ulnas of paired specimens were cemented with a cement gun or with a syringe. A 3D printed ulnar component was inserted and computed tomography scanning was performed on all specimens. Filling of the ulnar intramedullary canal was analysed using 3D reconstructions of the specimens. A greater degree of filling was seen by the use of the cement gun in 85.7%. Filling was 52.7% in the syringe group (25.1–78.7%), compared to 63.3% for the cement gun group ( p < 0.05). The use of a small nozzle cement gun provided a significantly higher filling degree of the ulnar canal. We recommend to always use a cement gun with a specific small nozzle to cement the ulnar component in total elbow arthroplasty.
Rapid Determination of 3 Components With Different Polarities in Medicinal Mushrooms by Multistep Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography Analysis
Authors: Qian Zhengming , Wu Zi , Li Chunhong , Yao ChangLiang , Tan Guoying , Li Wenjia , Guo De-an
Published: January 2021

In this study, a multistep matrix solid-phase dispersion (MSPD) combining with a high-performance liquid chromatography method was developed for assaying 3 components of different polarities (mannitol, adenosine, and ergosterol) from mushroom samples. MSPD extraction was carried out using 1 g octadecyl-bonded silica as the sorbent material, 9% methanol, 20% methanol, and 100% methanol as eluting solvents for the elution of mannitol, adenosine, and ergosterol, respectively. Mannitol was separated on an NH2P-50 4E column and detected using an evaporative light scattering detector. Adenosine and ergosterol were separated on a Poroshell 120 SB-C18 column and measured at 260 nm and 283 nm, respectively. The developed method showed good linearity ( R ≥ 0.9986) within the test range. The relative SD (RSD) of precisions were less than 1.4%, and the recoveries were 95.6%-97.0% (RSD ≤3.0%). Compared with the reported methods, the developed procedure could rapidly prepare components with different polarities (mannitol, adenosine, and ergosterol) from medical mushroom samples with less organic solvent and sample. The method is rapid and eco-friendly, which is helpful to improve the quality evaluation of medicinal mushrooms.
Management of the Tarsometatarsal Joint in the Rotational Correction of Hallux Valgus by the Modified Lapidus Procedure: Intraoperative Technical Tips to Prevent Complications
Authors: Nishikawa Danilo Ryuko Cândido , Saito Guilherme Honda , Mendes Alberto Abussamra Moreira , Prado Marcelo Pires
Published: January 2021

The modified Lapidus procedure is considered a useful tool for correction of multiplanar deformities in the hallux valgus, including first metatarsal pronation. It offers a greater power of correction compared to most other osteotomies. However, postoperative complications can occur in up to 12% of cases. The aim of this study was to describe intraoperative technical tips in the management of the tarsometatarsal joint during multiplanar correction of severe hallux valgus deformity using the modified Lapidus procedure. It is not the authors’ intention to describe a new technique, but to draw attention to intraoperative details in order to prevent complications as nonunion, extension of the first metatarsal and undercorrection of the deformity. Rotational correction of the first metatarsal with adequate bone coaptation of the first metatarsal and the medial cuneiform are the cornerstone for a satisfactory result.

Levels of Evidence: Level V, expert opinion
Impact damage protection mechanisms for elastomer-coated concrete
Authors: Fallon Chanel , McShane Graham J
Published: January 2021

Cost-efficient strategies for protecting structural elements against the effects of explosive detonations are of interest for vulnerable infrastructure. Dynamic loading due to both blast pressures and impact from fragments are of concern. This investigation focuses on the protection of concrete structural elements against impact damage. A recent experimental study by the authors demonstrated that an elastomer coating can provide a significant impact mitigating effect when applied to the impacted face of a concrete substrate. Preliminary numerical results have indicated that the elastomer serves to alter the details of damage initiation in the concrete, though there remains a limited understanding of the protective effects at play. In this work, a numerical investigation is performed to determine the mechanisms of impact damage initiation exhibited by a concrete circular cylinder of diameter, 100 mm and height, 100 mm when impacted by a 0.1 kg circular cylindrical (i.e. blunt) projectile, travelling at velocities in the range 5–150 m s−1. The influence of applying a 5 mm elastomer coating on these damage mechanisms is assessed. At the lowest impact velocities, the concrete remains undamaged, though the sub-surface stress state is influenced by the polymer coating. At higher impact velocities, two distinct damage initiation mechanisms are observed. Damage Mechanism 1 is characterised by immediate, severe concrete damage initiating under the indenter corner. Damage Mechanism 2 is characterised by more diffuse, sub-surface damage. Adding a polymer coating serves to shift damage initiation from Damage Mechanism 1–2, delaying the onset of severe concrete damage. Simplified 1D and 2D numerical models are employed to interrogate how the elastomer achieves this effect. Two protective effects are identified: (i) a temporal effect causing a reduction in the magnitude of peak acceleration and an increase in contact duration between projectile and target and (ii) a spatial effect where the stress concentration under the indenter corner is removed.
Surgical Fixation of Chondral-Only Fragments of the Knee: A Case Series With a Mean 4-Year Follow-up
Authors: Gudeman Andrew , Wischmeier Dillen , Farr Jack
Published: January 2021

Background:

Chondral-only fragments of the knee have traditionally been treated with excision, with or without cartilage restoration procedures. This is because of the historical assumption that cartilage has limited ability to heal to cancellous or subchondral bone. There is now a growing body of evidence supporting surgical fixation of these fragments.

Hypothesis:

We hypothesized that surgical fixation of chondral fragments would result in acceptable rates of healing with improvement in clinical outcome scores.

Study Design:

Case series; Level of evidence, 4.

Methods:

Data were collected on 15 surgically fixed chondral-only fragments in 14 patients. We retrospectively collected participant demographic information, lesion characteristics, primary mechanism (osteochondritis dissecans vs traumatic shear injury), fixation methods, reoperation information, second-look arthroscopic information, and clinical outcome scores. The mean clinical follow-up was 3.96 years, with a minimum of 1-year follow-up. All patients underwent follow-up magnetic resonance imaging at a mean of 2 years after the index procedure.

Results:

The mean age of our cohort was 17.7 years. We found an 80% survival rate for fixation of the fragments at a mean 4-year follow-up. There were statistically significant improvements in postoperative Knee injury and Osteoarthritis Outcome Score and Tegner scores compared with preoperative scores. Follow-up magnetic resonance imaging scans showed complete healing in 10 knees, partial healing in 2 knees, and loss of fixation in 3 knees. Second-look arthroscopic surgery of 3 knees for reasons other than fragment symptoms showed healing of the fragment, while arthroscopic surgery of 3 symptomatic knees showed loss of fixation.

Conclusion:

Surgical fixation of chondral-only lesions showed an 80% success rate with improvements in the KOOS and Tegner scores.
Confirming Proper Button Deployment of Suspensory Fixation During ACL Reconstruction
Authors: O’Brien Daniel F. , Fones Lilah , Stoj Victoria , Edgar Cory , Coyner Katherine , Arciero Robert A.
Published: January 2021

Background:

Suspensory fixation of anterior cruciate ligament (ACL) reconstruction (ACLR) grafts has emerged as a popular device for femoral graft fixation. However, improper deployment of the suspensory fixation can compromise proper graft tensioning, leading to failure and revision. Also, soft tissue interposition between the button and bone has been associated with graft migration and pain, occasionally requiring revision surgery. Many surgeons rely on manual testing and application of distal tension to the graft to confirm proper button deployment on the lateral cortex of the femur for ACL graft fixation.

Purpose:

To determine the reliability of the manual resistance maneuver when applying distal tension to deploy the suspensory device along the lateral cortex of the femur.

Study Design:

Case series; Level of evidence, 4.

Methods:

All patients undergoing ACLR with a suture button suspensory device for femoral fixation were eligible for enrollment in the study. The surgeries were performed by 3 board-certified, sports medicine fellowship–trained orthopaedic surgeons at a single outpatient surgical center between May 2018 and June 2019. All grafts were passed in a retrograde manner into the femoral tunnel, and a vigorous manual tensioning maneuver in a distal direction was placed on the graft to deploy and secure along the lateral cortex of the femur. Intraoperative mini c-arm fluoroscopy was obtained to demonstrate proper suture button positioning. If interposing tissue or an improperly flipped button was identified, rectifying steps were undertaken and recorded.

Results:

A total of 51 patients with a mean age of 33.3 years were included in the study. Of these patients, 74.5% had normal suture button positioning identified via intraoperative fluoroscopic imaging, while 15.7% had interposed soft tissue and 9.8% had an improperly flipped button. In all cases, the surgeon was able to rectify the malpositioning intraoperatively.

Conclusion:

Despite the manual sensation of proper suspensory button positioning, intraoperative fluoroscopy identified suture button deployment errors in ACLR 25% of the time. Correcting the malpositioning is not technically demanding. These findings advocate for routine intraoperative surveillance to confirm appropriate suture button seating during ACLR.
On the drivers of successful crowdfunding: The case of the platform Verkami
Authors: Martínez-Cháfer Luis , Molina-Morales Francesc Xavier , Peiró-Palomino Jesús
Published: January 2021

This article analyzes the determinants of successful crowdfunding initiatives using a sample of 5,251 projects from the reward-based Spanish platform Verkami. In contrast to most of the literature that has measured success with a dichotomous variable, we approach success with a continuous one, namely the ratio of achievement, which is measured as the obtained resources over the total amount requested by the promoter. We consider a set of potential determinants of success, which are theoretically well-grounded and cover a variety of spheres related to project features. Results suggest that factors related to the signaling theories such as partnership, having previous experience and interaction with backers are positively associated with the achievement ratio, whereas no significant impact is found for the number of rewards. In addition, we find uneven effects for low and high ratios of achievement, identifying the key determinants in different project stages.

JEL CLASSIFICATION D26; G29; L26; L86; M13; M21
Social Networks and Loneliness During the COVID-19 Pandemic
Authors: Kovacs Balazs , Caplan Nicholas , Grob Samuel , King Marissa
Published: January 2021

We utilize longitudinal social network data collected pre–COVID-19 in June 2019 and compare them with data collected in the midst of COVID in June 2020. We find significant decreases in network density and global network size following a period of profound social isolation. While there is an overall increase in loneliness during this era, certain social network characteristics of individuals are associated with smaller increases in loneliness. Specifically, we find that people with fewer than five “very close” relationships report increases in loneliness. We further find that face-to-face interactions, as well as the duration and frequency of interactions with very close ties, are associated with smaller increases in loneliness during the pandemic. We also report on factors that do not moderate the effect of social isolation on perceived loneliness, such as gender, age, or overall social network size.
The Racial Gap in Employment and Layoffs during COVID-19 in the United States: A Visualization
Authors: Dias Felipe A.
Published: January 2021

A large body of sociological research has shown that racial minorities and women experience significant disadvantages in the labor market. In this visualization, the author presents evidence from the Current Population Survey examining the effects of the coronavirus disease 2019 crisis on racial and gender inequalities in employment in the United States among prime-age workers. The author shows that the white-nonwhite gap in employment increased significantly during the post-outbreak period. Results from individual fixed-effects regression models show a strong white male advantage in the likelihood of being laid off for post-outbreak months compared with women, black men, Hispanic men, and Asian men.
Parkinson’s Patients’ Tolerance for Risk and Willingness to Wait for Potential Benefits of Novel Neurostimulation Devices: A Patient-Centered Threshold Technique Study
Authors: Hauber Brett , Mange Brennan , Zhou Mo , Chaudhuri Shomesh , Benz Heather L. , Caldwell Brittany , Ruiz John P. , Saha Anindita , Ho Martin , Christopher Stephanie , Bardot Dawn , Sheehan Margaret , Donnelly Anne , McLaughlin Lauren , Gwinn Katrina , Lo Andrew , Sheldon Murray
Published: January 2021

Background. Parkinson’s disease (PD) is neurodegenerative, causing motor, cognitive, psychological, somatic, and autonomic symptoms. Understanding PD patients’ preferences for novel neurostimulation devices may help ensure that devices are delivered in a timely manner with the appropriate level of evidence. Our objective was to elicit preferences and willingness-to-wait for novel neurostimulation devices among PD patients to inform a model of optimal trial design. Methods. We developed and administered a survey to PD patients to quantify the maximum levels of risks that patients would accept to achieve potential benefits of a neurostimulation device. Threshold technique was used to quantify patients’ risk thresholds for new or worsening depression or anxiety, brain bleed, or death in exchange for improvements in “on-time,” motor symptoms, pain, cognition, and pill burden. The survey elicited patients’ willingness to wait to receive treatment benefit. Patients were recruited through Fox Insight, an online PD observational study. Results. A total of 2740 patients were included and a majority were White (94.6%) and had a 4-year college degree (69.8%). Risk thresholds increased as benefits increased. Threshold for depression or anxiety was substantially higher than threshold for brain bleed or death. Patient age, ambulation, and prior neurostimulation experience influenced risk tolerance. Patients were willing to wait an average of 4 to 13 years for devices that provide different levels of benefit. Conclusions. PD patients are willing to accept substantial risks to improve symptoms. Preferences are heterogeneous and depend on treatment benefit and patient characteristics. The results of this study may be useful in informing review of device applications and other regulatory decisions and will be input into a model of optimal trial design for neurostimulation devices.
A Review of Special Economic Zone Led to Detrimental Implications: An Indian Experience
Authors: Parwez Sazzad
Published: January 2021

This article reviews the implications of special economic zones (SEZs) in the Indian context at both conceptual and empirical levels. It tries to draw attention toward detrimental modification in laws and actual implementation in the backdrop of judicial and executive practice. Implications in terms of socio-economic–legal–environmental taken together raise a question on the overall idea of SEZs. As evidences suggest, there are detrimental effects of SEZ operations in various forms.
Between nation and state: Boundary infrastructures, communities of practice and everyday nation-ness in the Chinese rail system
Authors: Brady Dylan
Published: January 2021

This paper draws on thinking within political geography and science and technology studies to examine how the infrastructure of the Chinese rail system situates the practices of individual passengers within the national community. Specifically, I employ Star’s boundary object framework to trace how hot water taps and their associated objects integrate multiple communities of practice into rail space while also producing fractures within the ridership. The practice of hot water drinking, like rail infrastructure, is the product of Chinese state-making. In contemporary China, however, thermoses and instant noodles have become markers not just of Chinese nation-ness but of particular sub-national communities of practice. I argue recent conflicts over their use and even presence in rail space reflect and realize fractures within broader society. This paper’s analysis of the Chinese rail system contributes to a clearer understanding of how top-down and bottom-up forces interact within China’s domestic infrastructural development. Incorporating the materiality of infrastructure allows political geographers to better understand how the nation-ness of certain bodies and practices are entangled with the built environment, moving from imagined communities to communities of practice.
Challenges for Rural Native American Students With Disabilities During COVID-19
Authors: Running Bear Candi , Terrill William P. A. , Frates Adriana , Peterson Patricia , Ulrich Judith
Published: January 2021

The COVID-19 pandemic unexpectedly changed almost all aspects of people’s everyday lives. This included new challenges in the education of Native American students with disabilities who live in rural and remote areas of the United States. Native American students with disabilities living on reservations are served by local schools, tribally controlled schools, and Bureau of Indian Education schools under the provisions of the Individuals with Disabilities Education Act. In rural reservation communities during COVID-19, special education services for students with disabilities were significantly disrupted. Contributing factors were high rates of poverty, lack of adequate funding and staffing for health care, populations with higher rates of chronic illness, high percentages of homes with no running water or electricity, shortages of certified special educators, and barriers to alternative forms of education due to lack of access to high-speed internet and technology.
A Tale of Two Countries: An International Perspective on Non-Traditional Special Education
Authors: Tekin-Iftar Elif , Jimenez Bree A. , Deniz Degirmenci Hatice
Published: January 2021

A global perspective of education, special education, rural communities, and non-traditional instruction is provided for two countries. Both Turkey and Australia have responded to the COVID-19 global pandemic in similar yet, different ways. Through the tale of two countries navigating the rapid response to school closure for students with disabilities, we all can gain understanding from examining strategies used by national, state, and local governments; school systems; and support agencies. From these strategies, we can determine effective and efficient models moving forward that support all students (e.g., students with mental health and medical needs) through non-traditional education during natural disasters, pandemics, or unexpected events
Nras
Q61R/+
and Kras
−/− cooperate to downregulate Rasgrp1 and promote lympho-myeloid leukemia in early T-cell precursors
Authors: Wen Zhi , Yun Grant , Hebert Alexander , Kong Guangyao , Ranheim Erik A. , Finn Remington , Rajagoplan Adhithi , Li Shuyi , Zhou Yun , Yu Mei , Damnernsawad Alisa , Roose Jeroen P. , Coon Joshua J. , Wen Renren , Wang Demin , Zhang Jing
Published: January 2021

Early T-cell precursor acute lymphoblastic leukemia (ETP-ALL) is an aggressive subtype of T-cell ALL. Although genetic mutations hyperactivating cytokine receptor/Ras signaling are prevalent in ETP-ALL, it remains unknown how activated Ras signaling contributes to ETP-ALL. Here, we find that in addition to the frequent oncogenic RAS mutations, wild-type (WT) KRAS transcript level was significantly downregulated in human ETP-ALL cells. Similarly, loss of WT Kras in NrasQ61R/+ mice promoted hyperactivation of extracellular signal-regulated kinase (ERK) signaling, thymocyte hyperproliferation, and expansion of the ETP compartment. Kras−/−; NrasQ61R/+ mice developed early onset of T-cell malignancy that recapitulates many biological and molecular features of human ETP-ALL. Mechanistically, RNA-sequencing analysis and quantitative proteomics study identified that Rasgrp1, a Ras guanine nucleotide exchange factor, was greatly downregulated in mouse and human ETP-ALL. Unexpectedly, hyperactivated Nras/ERK signaling suppressed Rasgrp1 expression and reduced Rasgrp1 level led to increased ERK signaling, thereby establishing a positive feedback loop to augment Nras/ERK signaling and promote cell proliferation. Corroborating our cell line data, Rasgrp1 haploinsufficiency induced Rasgrp1 downregulation and increased phosphorylated ERK level and ETP expansion in NrasQ61R/+ mice. Our study identifies Rasgrp1 as a negative regulator of Ras/ERK signaling in oncogenic Nras-driven ETP-like leukemia.
Serotyping of sub-Saharan Africa Salmonella strains isolated from poultry feces using multiplex PCR and whole genome sequencing
Authors: Kagambèga Assèta , Hiott Lari M. , Boyle David S. , McMillan Elizabeth A. , Sharma Poonam , Gupta Sushim K. , Ramadan Hazem , Cho Sohyun , Humayoun Shaheen B. , Woodley Tiffanie A. , Barro Nicolas , Jackson Charlene R. , Frye Jonathan G.
Published: January 2021

Background

Salmonella enterica remains a leading cause of food-borne diseases worldwide. Serotype information is important in food safety and public health activities to reduce the burden of salmonellosis. In the current study, two methods were used to determine serotypes of 111 strains of Salmonella isolated from poultry feces in Burkina Faso. First, Salmonella Multiplex Assay for Rapid Typing (SMART) Polymerase Chain Reaction (PCR) was used to determine the serovars of the S. enterica isolates. Second, serovar prediction based on whole genome sequencing (WGS) data was performed using SeqSero 2.0.

Results

Among the 111 Salmonella isolates, serotypes for 17 (15.31%) isolates were identified based on comparison to a panel of representative SMART codes previously determined for the 50 most common serovars in the United States. Forty-four (44) new SMART codes were developed for common and uncommon serotypes. A total of 105 (94.59%) isolates were serotyped using SeqSero 2.0 for serovar prediction based on WGS data.

Conclusion

We determined that SeqSero 2.0 was more comprehensive for identifying Salmonella serotypes from Burkina Faso than SMART PCR.
Comparative proteomic analysis of Nicotiana benthamiana plants under Chinese wheat mosaic virus infection
Authors: He Long , Jin Peng , Chen Xuan , Zhang Tian-Ye , Zhong Kai-Li , Liu Peng , Chen Jian-Ping , Yang Jian
Published: January 2021

Background

Chinese wheat mosaic virus (CWMV) is a severe threat to winter wheat and is transmitted by Polymyxa graminis. The mechanisms of interactions between CWMV and plants are poorly understood. In this study, a comparative proteomics analysis based on nanoliquid chromatography mass spectrometry (MS)/MS was conducted to characterize proteomic changes in plants responding to CWMV infection.

Results

In total, 2751 host proteins were identified, 1496 of which were quantified and 146 up-regulated and 244 down-regulated proteins were identified as differentially expressed proteins (DEPs). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that DEPs were most strongly associated with photosynthesis antenna proteins, MAPK signaling plant and glyoxylate and dicarboxylate metabolism pathways. Subcellular localization analysis predicted that more than half of the DEPs were localized in the chloroplast, an organelle indispensable for abscisic acid (ABA) synthesis. Our results suggest that CWMV infection interrupts normal chloroplast functions and decreases ABA concentrations in Nicotiana benthamiana. Further analysis showed that the ABA pathway was suppressed during CWMV infection and that ABA treatment induced plant hosts defenses against CWMV.

Conclusions

We identified several candidate proteins expressed during CWMV infection, and the ABA pathway was strongly associated with responses to CWMV infection in N. benthamiana.
Sex-specific typologies of older adults’ sedentary behaviors and their associations with health-related and socio-demographic factors: a latent profile analysis
Authors: Compernolle Sofie , De Bourdeaudhuij Ilse , Cardon Greet , Van Dyck Delfien
Published: January 2021

Background

Some types of sedentary behaviors tend to cluster in individuals or groups of older adults. Insight into how these different types of sedentary behavior cluster is needed, as recent research suggests that not all types of sedentary behavior may have the same negative effects on physical and mental health. Therefore, the aim of this study was to identify sex-specific typologies of older adults’ sedentary behavior, and to examine their associations with health-related and socio-demographic factors.

Methods

Cross-sectional data were collected as part of the BEPAS Seniors, and the Busschaert study among 696 Flemish older adults (60+). Typologies of self-reported sedentary behavior were identified using latent profile analysis, and associations with health-related and sociodemographic factors were examined using analyses of variances.

Results

Five distinct typologies were identified from seven sedentary behaviors (television time, computer time, transport-related sitting time, sitting for reading, sitting for hobbies, sitting for socializing and sitting for meals) in men, and three typologies were identified from six sedentary behaviors (television time, transport-related sitting time, sitting for reading, sitting for hobbies, sitting for socializing and sitting for meals) in women. Typologies that are characterized by high television time seem to be related to more negative health outcomes, like a higher BMI, less grip strength, and a lower physical and mental health-related quality-of-life. Typologies that are represented by high computer time and motorized transport seem to be related to more positive health outcomes, such as a lower body mass index, more grip strength and a higher physical and mental health-related quality-of-life.

Conclusions

Although causal direction between identified typologies and health outcomes remains uncertain, our results suggests that future interventions should better focus on specific types of sedentary behavior (e.g. television time), or patterns of sedentary behavior, rather than on total sedentary behavior.
Molecular characteristics of HBV infection among blood donors tested HBsAg reactive in a single ELISA test in southern China
Authors: Ye Xianlin , Li Tong , Li Ran , Liu Heng , Zhao Junpeng , Zeng Jinfeng
Published: January 2021

Background

Hepatitis B virus (HBV) infection is a major concern for blood safety in high-prevalence HBV countries such as China. In Shenzhen, dual hepatitis B surface antigen (HBsAg) enzyme-linked immunosorbent assays (ELISAs) have been adopted in parallel with nucleic acid testing (NAT) for donors for over a decade. A small proportion of blood donors test reactive (R) for HBsAg but negative through routine NAT, which can lead to HBV infection with an extremely low viral load.

Objectives

We aimed to investigate and analyze the molecular characteristics of HBV among blood donors that tested HBsAg R in a single ELISA test.

Methods

Blood donations were evaluated in this study if confirmed HBsAg R through one of two ELISA kits. Samples with non-reactive (NR) results by NAT were collected and tested for HBsAg by chemiluminescent microparticle immunoassay (CLIA) with a neutralization test. The level of HBsAg was further assessed by electrochemiluminescence immunoassay (ECLIA). The viral basic core promoter (BCP) and pre-core (PC) and S regions were amplified by nested PCR. Quantitative real-time PCR (qPCR) for viral load determination and individual donation (ID)-NAT were adopted simultaneously. HBsAg was confirmed with CLIA, ECLIA, nested PCR, qPCR, and ID-NAT.

Results

Of the 100,252 donations, 38 and 41 were identified as HBsAg R with Wantai and DiaSorin ELISA kits, respectively. Seventy-nine (0.077%, 79/100,252) blood samples with ELISA R-NR and NAT NR results were enrolled in the study. Of these, 17 (21.5%,17/79) were confirmed as HBsAg-positive. Of the 14 genotyped cases, 78.6% (11/14) were genotype B, and C and D were observed in two and one sample, respectively. Mutations were found in the S gene, including Y100C, Y103I, G145R, and L175S, which can affect the detection of HBsAg. A high-frequency mutation, T1719G (93.3%), was detected in the BCP/PC region, which reduced the viral replication.

Conclusion

A small number of blood samples with HBsAg ELISA R-NR and NAT NR results were confirmed as HBV infection, viral nucleic acids were found in most of the samples through routine NAT methods. It is necessary to employ more sensitive and specific assays for the detection of HBV infection among blood donors.
Busy day effect on intrapartum adverse maternal outcomes – a population‐based study of 601 247 singleton deliveries
Authors: Vilkko Riitta , Räisänen Sari , Gissler Mika , Stefanovic Vedran , Heinonen Seppo
Published: January 2021

Background

This was a retrospective population-based study, utilizing the data of 601 247 singleton hospital deliveries collected from the Finnish Medical Birth Register (MBR) in 2006–2016. The aim of this study was to analyse the busy day effect on intrapartum adverse maternal outcomes.

Methods

To implement the study design, daily delivery frequencies and ranges (min-max) for each delivery unit (n = 26) were stratified to the daily delivery volume distributions by the delivery unit’s annual delivery volume and profile: Category (C)1 < 1000, C2 1000–1999, C3 2000–2999, C4 ≥ 3000 and C5 the profile of university hospitals. To study the busy day effect, the quiet, optimal and busy days were defined by calculating the number of days (%) with the lowest and highest daily delivery frequencies and summed to the nearest 10 % in each hospital category. Optimal days were determined by calculating approximately 80 % of deliveries occurring between the lowest 10 %, and highest 10 % in each hospital category. Crude and adjusted odd ratios (ORs) with 99 % confidence intervals (CIs) were used to analyze the busy day effect on adverse maternal outcomes, blood transfusions, manual removal of the placenta and obstetric anal sphincter injuries, separately in each hospital category.

Results

The busy day effect was associated with the 28 % (99 % CI 8–52 %) and 25 % (99 % CI 11–40 %) increased need for blood transfusions in C2 and university hospitals (C5), respectively, whereas 22 % (99 % CI 10–31 %) less blood transfusions were needed at university hospitals during quiet days. In C3 hospitals, 83 % (99 % CI 65–92 %) less blood transfusions were needed during busy days. Obstetric and anal sphincter injury rates declined during quiet days by 22 % (99 % CI 3–38 %) only in university hospitals.

Conclusions

The findings of this study identify no specific pattern to the busy day effect for adverse maternal outcomes defined as manual removal of the placenta or obstetric and anal sphincter injuries. However, both quiet and busy days seem to be associated with increased or decreased need for blood transfusions in different sized delivery units. Findings also suggest that quiet days are associated with a decreased number of obstetric and anal sphincter injuries.
Comparative analysis of patients with upper urinary tract urothelial carcinoma in black-foot disease endemic and non-endemic area
Authors: Chang Che-Wei , Ou Chien-Hui , Yu Chih-Chin , Lo Chi-Wen , Tsai Chung-You , Cheng Pai-Yu , Chen Yung-Tai , Huang Hsu-Che , Wu Chia-Chang , Li Ching-Chia , Lee Hsiang-Ying
Published: January 2021

Background

A high incidence of upper urinary tract urothelial carcinoma has been reported in the southwestern area of Taiwan, where arsenic water contamination was considered the main cause. However, there is no definite proof to show a correlation between arsenic water contamination and upper urinary tract urothelial carcinoma. To investigate the clinical and epidemiological features of patients with upper urinary tract urothelial carcinoma between arsenic water endemic and non-endemic areas, we analyzed patients in terms of characteristics, stratified overall survival, disease-free survival, and cancer-specific survival.

Methods

The records of a total of 1194 patients diagnosed with upper urinary tract urothelial carcinoma were retrospectively reviewed. Clinical data and current medical status were collected from the medical records. Statistical analyses were performed to determine the clinical variables and stratified survival curves between endemic and non-endemic groups.

Results

Female predominance was revealed in both endemic and non-endemic groups (male:female ratio = 1:1.2–1.4). No statistical differences were found in histological types, staging, and tumor size between the two groups. Nonetheless, patients with characteristics of aging and having end-stage renal disease were outnumbered in the non-endemic group, while a higher prevalence of previous bladder tumors and more ureteral tumors were found in the endemic group. Adjusted stratified cumulative survival curves suggested a poorer prognosis in endemic patients, especially in disease-free survival of early stage disease.

Conclusions

A higher mortality rate with more previous bladder cancer history and ureteral tumors was seen in patients with upper urinary tract urothelial carcinoma residing in the arsenic water contamination area. This may be attributed to the long-term carcinogenic effect of arsenic underground water.
The possible causes for tomography suspect Keratoconus in a Chinese cohort
Authors: Feng Kang , Zhang Yu , Chen Yue-guo
Published: January 2021

Background

To explore the possible causes of tomography suspect keratoconus (TSK) marked by Tomography in screening keratoconus in a Chinese cohort, and the reasonable range of corneal horizontal diameter and thickness for decreasing the proportion of TSK.

Methods

Nested case-control study from a single center prospective cohort. All subjects were selected from the Peking University Third Hospital Ectasia Cornea Disease Cohort Project database, which included myopic patients seeking corneal refractive surgical corrections since 2013. Demographic information, basic eye examination, and auxiliary equipment examination including refraction, IOL-master, Pentacam, Sirius, and Topolyzer were recorded. In this study, all cases were classified into two groups: TSK group and normal control (NC) group, and all of them were followed up at least 2 years. The former is consisted of those whose screening examinations of tomography are abnormal, the latter is those whose screening examinations are normal. All of them have already been followed up at least 2 years without abnormalities after excimer laser corneal refractive surgeries. Unpaired t tests and Chi-square tests were used to compare the differences of indices from the tomography between the two groups.

Results

Of 183 TSK eyes (109 patients) and 160 NC eyes (83 patients), the mean age is 28.0 and 26.3 years old respectively. The corneal horizontal diameter is 11.5–11.8 mm in TSK group and 11.8–12.0 mm in NC group. The central corneal thickness is nearly 520 μm in the former and 550 μm in the latter. For Sirius, the TSK ratio of indices of SIf and SIb is 41.5 and 39.9% respectively in TSK group. For Pentacam, the TSK ratio of index IHD is 59.0% and “final D” is 72.7%.

Conclusions

Corneal horizontal diameter and central corneal thickness have great influences on the results of corneal tomography in detecting the suspect keratoconus.
Reproductive intentions and corresponding use of safer conception methods and contraception among Ugandan HIV clients in serodiscordant relationships
Authors: Wagner Glenn J. , Mindry Deborah , Hurley Emily A. , Beyeza-Kashesya Jolly , Gwokyalya Violet , Finocchario-Kessler Sarah , Wanyenze Rhoda K. , Nanfuka Mastula , Tebeka Mahlet G. , Goggin Kathy
Published: January 2021

Context

Among people living with HIV in Uganda, desires to have a child and unplanned pregnancies are both common, while utilization of safer conception methods (SCM) and modern contraceptives are low.

Methods

Three hundred eighty-nine HIV clients who reported considering childbearing with their uninfected partner enrolled in a safer conception counseling intervention trial in Uganda. Multiple regression analysis and baseline data were used to examine correlates of reproductive intentions and behaviors, including use of safer conception methods and contraception.

Results

Most (n = 313; 80.5%) reported that both they and their partner wanted to have a child now, which was associated with being married, in a longer relationship, not having a child with partner, greater SCM knowledge, lower internalized childbearing stigma, and higher perceived community stigma of childbearing. However, just 117 reported trying to conceive in the prior 6 months, which was associated with being female, not having a child with their partner, less decision-making control within the relationship, and greater perceived cultural acceptability of SCM. Among those who had tried to conceive in the past 6 months, 14 (11.9%) used SCM, which was associated with greater control in decision making. Of the 268 who were not trying to conceive, 69 (25.7%) were using a modern contraceptive, which was associated with being in a shorter relationship, less control over decision-making, more positive attitudes towards contraception and lower depression.

Conclusion

Methods to promote reproductive goals are underused by HIV serodiscordant couples, and relationships characteristics and childbearing-related stigma appear to be most influential and thus targets for intervention.

Trial registration

Clinicaltrials.gov, NCT03167879; date registered May 23, 2017.