bluuebunny/crossref_metadata_2025 huggingface dataset:
The Ernesto Cave, northern Italy, as a candidate auxiliary reference section for the definition of the Anthropocene series
Authors: Borsato Andrea , Fairchild Ian J , Frisia Silvia , Wynn Peter M , Fohlmeister Jens
Published: January 2023

Annually laminated stalagmites ER77 and ER78 from Grotta di Ernesto provide an accurate annual record of environmental and anthropogenic signals for the last ~200 years. Two major transitions are recorded in the stalagmites. The first coincides with the year 1840 CE, when a change from porous and impurity-rich-laminae to clean, translucent laminae occurs. This is accompanied by a steady increase in the growth rate, a decrease in fluorescence and a sharp increase in δ13C values. These changes concur with the end of the Little Ice Age. The second transition takes place around the year 1960 CE and corresponds with an increase in both annual growth rate and sulfur concentration in stalagmite ER78 at 4.2 mm from the top, and with the deflection point in the 14C activity curve in stalagmite ER77 at 4.8 mm from the top. This latter is the stratigraphic signal proposed as the primary guide for the definition of the Anthropocene series. The following shift toward depleted δ34S–SO4 in stalagmite ER78 suggests that industrial pollution is a major source of sulfur. The interpretation of atmospheric signals (S, δ34S, 14C) in the stalagmites is affected by attenuation and time lags and the environmental signals are influenced by soil and ecosystem processes, while other anthropogenic signals (δ15N, 239Pu) are not recorded. For these reasons, the stalagmite record is here proposed as an auxiliary (reference) section rather than a global standard. In summary, Grotta di Ernesto contains one of the best stalagmite records documenting the Anthropocene, and one of only two stalagmite records where the S peak has been measured at high resolution.
Heartbeat sound classification using a hybrid adaptive neuro-fuzzy inferences system (ANFIS) and artificial bee colony
Authors: Keikhosrokiani Pantea , Naidu A/P Anathan A. Bhanupriya , Iryanti Fadilah Suzi , Manickam Selvakumar , Li Zuoyong
Published: January 2023

Cardiovascular disease is one of the main causes of death worldwide which can be easily diagnosed by listening to the murmur sound of heartbeat sounds using a stethoscope. The murmur sound happens at the Lub-Dub, which indicates there are abnormalities in the heart. However, using the stethoscope for listening to the heartbeat sound requires a long time of training then only the physician can detect the murmuring sound. The existing studies show that young physicians face difficulties in this heart sound detection. Use of computerized methods and data analytics for detection and classification of heartbeat sounds will improve the overall quality of sound detection. Many studies have been worked on classifying the heartbeat sound; however, they lack the method with high accuracy. Therefore, this research aims to classify the heartbeat sound using a novel optimized Adaptive Neuro-Fuzzy Inferences System (ANFIS) by artificial bee colony (ABC). The data is cleaned, pre-processed, and MFCC is extracted from the heartbeat sounds. Then the proposed ABC-ANFIS is used to run the pre-processed heartbeat sound, and accuracy is calculated for the model. The results indicate that the proposed ABC-ANFIS model achieved 93% accuracy for the murmur class. The proposed ABC-ANFIS has higher accuracy in compared to ANFIS, PSO ANFIS, SVM, KSTM, KNN, and other existing studies. Thus, this study can assist physicians to classify heartbeat sounds for detecting cardiovascular disease in the early stages.
Drinker Identity Development: Shame, Pride, and a Thirst to Belong
Authors: Uscola Colter J.
Published: January 2023

Identity theorists assume that individuals intentionally construct and maintain a culturally valued sense of self. Although this logic makes sense for positive identities—doctor, parent, or scientist—it becomes questionable when applied to the construction of negative, or stigmatized, identities, such as that of a drinker. By interviewing 16 members of a metropolitan recovery community, I focus on how marginalized identities form seemingly absent of intention. In doing so, I show how stress and negative messaging from guardians, peers, and community members produce persistent painful emotions that restrict access to culturally valued identity pathways and steer individuals toward spaces of consumption. Through each lost socially valued role, the drinker identity becomes more salient, achieving more importance in daily life and becoming central to individuals’ lived experiences. That is, the drinker role becomes a primary source of positive affect and belongingness when these essential ingredients of social life are unobtainable elsewhere. More broadly, I challenge current theoretical assumptions that dominated intervention strategies and recovery policy for decades and offer considerations for policy and intervention programs.
Return to Play After Shoulder Surgery in Professional Baseball Players: A Systematic Review and Meta-analysis
Authors: Giberson-Chen Carew C. , Shaw Brian L. , Rudisill Samuel S. , Carrier Robert E. , Farina Evan M. , Pearson Brad , Asnis Peter D. , O’Donnell Evan A.
Published: January 2023

Background:

The current literature lacks an updated review examining return to play (RTP) and return to prior performance (RTPP) after shoulder surgery in professional baseball players.

Purpose:

To summarize the RTP rate, RTPP rate, and baseball-specific performance metrics among professional baseball players who underwent shoulder surgery.

Study Design:

Systematic review; Level of evidence, 4.

Methods:

A literature search was performed utilizing the PubMed, MEDLINE, and CINAHL databases and according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Inclusion criteria were English-language studies reporting on postoperative RTP and/or RTPP in professional baseball players who underwent shoulder surgery between 1976 and 2016. RTP rates, RTPP rates, and baseball-specific performance metrics were extracted from qualifying studies. A total of 2034 articles were identified after the initial search. Meta-analysis was performed where applicable, yielding weighted averages of RTP and RTPP rates and comparisons between pitchers and nonpitchers for each type of surgery. Baseball-specific performance metrics were reported as a narrative summary.

Results:

Overall, 26 studies featuring 1228 professional baseball players were included. Patient-level outcome data were available for 529 players. Surgical interventions included rotator cuff debridement (n = 197), rotator cuff repair (RCR; n = 43), superior labrum from anterior to posterior repair (n = 124), labral repair (n = 103), latissimus dorsi/teres major (LD/TM) repair (n = 21), biceps tenodesis (n = 17), coracoclavicular ligament reconstruction (n = 15), anterior capsular repair (n = 5), and scapulothoracic bursectomy (n = 4). Rotator cuff debridement was the most common surgical procedure, while scapulothoracic bursectomy was the least common (37.2% and 0.8% of interventions, respectively). Meta-analysis revealed that the RTP rate was highest for LD/TM repair (84.5%) and lowest for RCR (53.5%), while the RTPP rate was highest for LD/TM repair (100.0%) and lowest for RCR (27.9%). RTP and RTPP rates were generally higher for position players than for pitchers. Nonvolume performance metrics were unaffected by shoulder surgery, while volume statistics decreased or remained similar.

Conclusion:

RTP and RTPP rates among professional baseball players were modest after most types of shoulder surgery. Among surgical procedures commonly performed on professional baseball players, RTP and RTPP rates were highest for LD/TM repair and lowest for RCR.
Discrepancies in Objective and Subjective Cognition in Middle-Aged and Older Adults: Does Personality Matter?
Authors: Costa Amy N. , Nowakowski Lauren M. , McCrae Christina S. , Cowan Nelson , Curtis Ashley F.
Published: January 2023

Associations between subjective cognition and current objective functioning are inconclusive. Given known associations between personality and cognition, this study tested whether personality moderates associations between subjective memory and objective cognition in middle-aged and older adults. Participants ( N = 62, Mage = 63.8, SD = 7.7, 33 men) completed assessments of personality (Big Five Inventory-10), subjective memory (Cognitive Failures Questionnaire [CFQ-memory]), and objective cognition (processing speed, attention, inhibition [Stroop], working memory [Sternberg], set-shifting [Wisconsin Card Sorting Task]). Multiple regressions and simple slopes analyses examined whether personality moderates associations between subjective memory and objective cognition, controlling for age, number of medical conditions, and household income. Extraversion moderated associations between processing speed and CFQ-memory. Agreeableness moderated associations between set-shifting and CFQ-memory. Among individuals with higher extraversion and lower agreeableness, objectively worse cognition was associated with the fewest memory complaints. Findings suggest personality may impact the discrepancies between subjective memory and objective cognition in mid-to-late life.
Managers’ and Administrators’ Perspectives on Digital Technology Use in Regional Long-Term Care Homes During the COVID-19 Pandemic
Authors: Khowaja Asif , Syed Nawal , Michener Kaitlyn , Mechelse Kristin , Koning Henriette
Published: January 2023

In this paper, we explore managers’ and administrators’ perspectives on digital technology use for residents during province-wide lockdowns (June–August 2021) during the COVID-19 pandemic in seven regional long-term care homes (LTC) in Niagara, Canada. Fifteen semi-structured interviews were conducted with participants representing operational, financial, and recreational departments where we discussed their needs and factors influencing the use of digital technology during the phases of increased restrictions on visitors and social isolation. Our findings indicate extensive use of cellular devices including smartphones, however additional iPads were needed to meet the ever-rising demand for virtual connections. Almost all participants revealed supportive leadership, redeployed staff, and community donations as main facilitators for technology use. Barriers related to managing varying elderly cognitive capacities and technical issues affected technology use. Based on our findings, we conclude that financial commitment and community support are integral for future-proofing LTC homes with technological innovations.
Classroom Assessment in Higher Education
Authors: Rao N. J. , Banerjee Shilpi
Published: January 2023

Classroom assessment is the process of documenting the knowledge, skills, attitudes and beliefs of learners. It provides essential feedback to both instructors and students to improve their teaching methods for guiding and motivating students to be actively involved in their learning. Assessment drives learning. Formative assessments enable the instructor to guide the students to learn well. Summative assessments enable the measurement of levels of attainment of course outcomes and act as feedback to course design and curriculum improvement. This article presents the underlying principles of assessment through a discussion of assessment approaches and their purposes, types of assessment items, quality of assessment and summative assessment plans. Quality assessment instruments can be developed through an understanding of the quality attributes of assessment items, the process of designing assessment instruments, designing a variety of assessment items, and devising plans to evaluate them through rubrics. An approach is presented for creating a summative assessment plan that can also lead to the attainment of outcomes as per the requirements of programme accreditation.
A network-based analysis to assess COVID-19 disruptions in the Bogotá BRT system
Authors: Garcia-Arteaga Juan D. , Lotero Laura
Published: January 2023

The global COVID-19 crisis has severely affected mass transit in the cities of the global south. Fear of widespread propagation in public spaces and the dramatic decrease in human mobility due to lockdowns have resulted in a significant reduction of public transport options. We analyze the case of TransMilenio in Bogotá, a massive Bus Rapid Transit system that is the main mode of transport for an urban area of roughly 10 million inhabitants. Concerns over social distancing and new health regulations reduced the number of trips to under 20% of its historical values during extended periods of time during the lockdowns. This has sparked a renewed interest in developing innovative data-driven responses to COVID-19 resulting in large corpora of TransMilenio data being made available to the public. In this paper we use a database updated daily with individual passenger card swipe validation microdata including entry time, entry station, and a hash of the card’s ID. The opportunity of having daily detailed minute-to-minute ridership information and the challenge of extracting useful insights from the massive amount of raw data (∼1,000,000 daily records) require the development of tailored data analysis approaches. Our objective is to use the natural representation of urban mobility offered by networks to make pairwise quantitative similarity measurements between daily commuting patterns and then use clustering techniques to reveal behavioral disruptions as well as the most affected geographical areas due to the different pandemic stages. This method proved to be efficient for the analysis of large amount of data and may be used in the future to make temporal analysis of similarly large datasets in urban contexts.
‘Often in between’: Thinking through research methods and Indigenous sovereignty with Yuin Country
Authors: Arnold Crystal , Atchison Jennifer , McKnight Anthony
Published: January 2023

Indigenous scholars have been calling for renewed attention to the theorisation and practice of sovereignty, including within research. Their scholarship has drawn attention to the sovereignty of people within research processes, as well as diverse expressions of sovereignty. In this article we bring these two dimensions into conversation to consider how research methods might enable and enliven understandings and practices of sovereignty within geography. Illustrating Yuin approaches to research through poetry, observation and dreaming, we show how Country is a living enactment of sovereignty. It dynamically contributes to research processes and enables nonhuman entities to communicate within and beyond their territory as sovereign subjects. In particular, Yuin research methods acknowledge the significant contribution of plants in the theorisation~storying and practice of sovereign Country. Through plants, we come to develop a knowledge of sovereign Country as often in between things, including places and knowledges, vulnerability and protection, and removal and persistence. Such insights are respectfully offered here in the spirit of broadening disciplinary perspectives and capacities in order to revitalise research that addresses the relationships between territory and its people.
Comparing two mathematical competencies frameworks from China and Denmark
Authors: Hong Yanjun , Jankvist Uffe Thomas
Published: January 2023

In this article, we compare two disciplinary competency descriptions of mathematics. More precisely, we describe and compare the Core Mathematics Competencies (CMC) framework in the Chinese mainland with the Danish mathematics competencies framework (KOM). We look for similarities and differences in the conceptualization of “competency”, the structure of the two frameworks as well in their specific competency descriptions and ask the question of how to articulate any identified similarities and differences. Relying on a distinction between disciplinary competency descriptions for internal and for external use, we conclude that while KOM is to be regarded as a more or less classical competency description for internal use, that is, one that facilitates communication between various practitioners within a discipline, CMC encompasses more aspects of a competency description for external use, that is, it also facilitates communication between practitioners and recipients.
Analysis of immune responses in patients with CLL after heterologous COVID-19 vaccination
Authors: Lee Hye Kyung , Hoechstetter Manuela A. , Buchner Maike , Pham Trang Thu , Huh Jin Won , Müller Katharina , Zange Sabine , von Buttlar Heiner , Girl Philipp , Wölfel Roman , Brandmeier Lisa , Pfeuffer Lisa , Furth Priscilla A. , Wendtner Clemens-Martin , Hennighausen Lothar
Published: January 2023

Patients with chronic lymphocytic leukemia (CLL) treated with B-cell pathway inhibitors and anti-CD20 antibodies exhibit low humoral response rates following SARS-CoV-2 vaccination. To investigate this observation, a prospective single-institution study was conducted comparing peripheral blood mononuclear cell transcriptional response with antibody and T-cell response rates following heterologous BNT162b2/ChAdOx1 vaccination of 15 patients with CLL/small lymphocytic lymphoma (SLL). Two-dose antibody response rate was 40%, increasing to 53% after booster. Patients on Bruton tyrosine kinase inhibitor (BTKi) and venetoclax ± anti-CD20 antibody within 12 months of vaccination responded inferiorly compared with those under BTKi alone. The 2-dose–T-cell response rate was 80%, which increased to 93% after the booster dose. Key transcriptional findings were that interferon–mediated signaling activation including activation of the JAK-STAT pathway generally occurred within days of vaccination, but was independent from the magnitude of the antibody response. Increasing counts of IGHV genes were associated with B-cell reconstitution and improved humoral response rate in the vaccinated patients. T-cell responses in patients with CLL appeared independent of treatment status, whereas higher humoral response rate was associated with BTKi treatment and B-cell reconstitution. Boosting was particularly effective when intrinsic immune status was improved by CLL treatment. Limitations included studying a relatively small cohort, with different treatments and vaccination schedules.
Endotyping COPD: hypoxia-inducible factor-2 as a molecular “switch” between the vascular and airway phenotypes?
Authors: Myronenko Oleh , Foris Vasile , Crnkovic Slaven , Olschewski Andrea , Rocha Sonia , Nicolls Mark R. , Olschewski Horst
Published: January 2023

COPD is a heterogeneous disease with multiple clinical phenotypes. COPD endotypes can be determined by different expressions of hypoxia-inducible factors (HIFs), which, in combination with individual susceptibility and environmental factors, may cause predominant airway or vascular changes in the lung. The pulmonary vascular phenotype is relatively rare among COPD patients and characterised by out-of-proportion pulmonary hypertension (PH) and low diffusing capacity of the lung for carbon monoxide, but only mild-to-moderate airway obstruction. Its histologic feature, severe remodelling of the small pulmonary arteries, can be mediated by HIF-2 overexpression in experimental PH models. HIF-2 is not only involved in the vascular remodelling but also in the parenchyma destruction. Endothelial cells from human emphysema lungs express reduced HIF-2α levels, and the deletion of pulmonary endothelialHif-2α leads to emphysema in mice. This means that both upregulation and downregulation of HIF-2 have adverse effects and that HIF-2 may represent a molecular “switch” between the development of the vascular and airway phenotypes in COPD. The mechanisms of HIF-2 dysregulation in the lung are only partly understood. HIF-2 levels may be controlled by NAD(P)H oxidasesviairon- and redox-dependent mechanisms. A better understanding of these mechanisms may lead to the development of new therapeutic targets.
Complexity vs linearity: relations between functional traits in a heterotrophic protist
Authors: Svendsen Nils A. , Radchuk Viktoriia , Morel-Journel Thibaut , Thuillier Virginie , Schtickzelle Nicolas
Published: January 2023

Background

Functional traits are phenotypic traits that affect an organism’s performance and shape ecosystem-level processes. The main challenge when using functional traits to quantify biodiversity is to choose which ones to measure since effort and money are limited. As one way of dealing with this, Hodgson et al. (Oikos 85:282, 1999) introduced the idea of two types of traits, with soft traits that are easy and quick to quantify, and hard traits that are directly linked to ecosystem functioning but difficult to measure. If a link exists between the two types of traits, then one could use soft traits as a proxy for hard traits for a quick but meaningful assessment of biodiversity. However, this framework is based on two assumptions: (1) hard and soft traits must be tightly connected to allow reliable prediction of one using the other; (2) the relationship between traits must be monotonic and linear to be detected by the most common statistical techniques (e.g. linear model, PCA).

Results

Here we addressed those two assumptions by focusing on six functional traits of the protist species Tetrahymena thermophila, which vary both in their measurement difficulty and functional meaningfulness. They were classified as: easy traits (morphological traits), intermediate traits (movement traits) and hard traits (oxygen consumption and population growth rate). We detected a high number (> 60%) of non-linear relations between the traits, which can explain the low number of significant relations found using linear models and PCA analysis. Overall, these analyses did not detect any relationship strong enough to predict one trait using another, but that does not imply there are none.

Conclusions

Our results highlighted the need to critically assess the relations among the functional traits used as proxies and those functional traits which they aim to reflect. A thorough assessment of whether such relations exist across species and communities is a necessary next step to evaluate whether it is possible to take a shortcut in quantifying functional diversity by collecting the data on easily measurable traits.
Patients with indolent lymphomas are at high risk of infections: experience from a German outpatient clinic
Authors: Lutz Christoph , Feiten Stefan , Chakupurakal Geothy , Heymanns Jochen , Thomalla Jörg , van Roye Christoph , Weide Rudolf
Published: January 2023

Background

Patients with indolent B-cell non-Hodgkin lymphomas (B-NHLs) have an increased risk of infections which is caused by pathomechanisms of the diseases itself but also as a result of anti-tumor therapy. Especially the effects of anti-CD20 antibodies are well understood as these lead to decreased antibody production. Most studies regarding immunodeficiency in B-NHLs were conducted with multiple myeloma and chronic lymphocytic leukemia patients. As these studies not always represent the general population we collected and analyzed real world data from patients with indolent lymphomas and a control group (CG).

Results

Patients with B-NHLs undergoing therapy or who were regularly monitored in a watch and wait approach had, over the time of one year, an increased rate of infections compared to the CG of 145 healthy volunteers (mean: 11.66 vs. 7.13 infections per 1000 days). Consistent with this finding B-NHL patients received more antibiotic treatment (mean: 11.17 vs. 6.27 days) and were more often hospitalized than persons from the CG (mean: 5.19 vs. 0.99 days per 1000 days). Lymphoma patients without immunodeficiency had a lower infection rate than patients with non-symptomatic and symptomatic immunodeficiency (mean: 10.91 vs. 12.07 and 12.36 per 1000 days). The number of infections differed statistically significant for the subgroups and CG (7.13 per 1000 days). Patients with symptomatic immunodeficiency were mostly treated with regular immunoglobulin substitutions and infection rates were comparable to those of patients with asymptomatic immunodeficiency.

Conclusions

Our data suggest the use of an approach with regular immune monitoring including the measurement of immunoglobulin levels and regular appointments for clinical assessment of all indolent lymphoma patients in order to identify patients with increased risk of infections. It also raises the question if patients with immunodeficiency should be treated more often with regular immunoglobulin substitution, but so far more studies are necessary to answer this question.
CTLA4+CD4+CXCR5−FOXP3+ T cells associate with unfavorable outcome in patients with chronic HBV infection
Authors: Wen Chunhua , Dong Zheyu , Wang Yiyue , Ye Guofu , Ma Yanchen , Yi Xuan , Zhou Yang , Li Xiaoyi , Zheng Xinchun , Hou Jinlin , Li Yongyin , Tang Libo
Published: January 2023

Background

A major barrier to achieving a favorable outcome of chronic HBV infection is a dysregulated HBV-specific immune response resulting from immunosuppressive features of FOXP3+ T cells. A better definition of FOXP3+ T cells is essential for improving the prognosis of HBV infection. We aimed to investigate the role of CD4+CXCR5−FOXP3+ T cells with CTLA4 expression in patients with chronic HBV infection.

Methods

Treatment-naïve chronic HBV-infected patients, HBV-related hepatic failure, and a longitudinal cohort of chronic hepatitis B (CHB) patients with nucleos(t)ide analogue treatment were enrolled for analysis of CD4+CXCR5−FOXP3+ T cell responses by flow cytometry and single-cell RNA sequencing (scRNA-seq).

Results

ScRNA-seq revealed that circulating CD4+CXCR5−FOXP3+ T cells presented distinct inhibitory features compared to spleen tissue. Meanwhile, patients with treatment-naïve chronic HBV infection or with HBV-related hepatic failure showed an upregulation of immune-suppressive features (PD-1, CTLA4, GITR) on CD4+CXCR5−FOXP3+T cells; in vitro analysis found HBeAg and HBcAg stimulation induced elevated levels of inhibitory molecules. Notably, the frequency of CTLA4+CD4+CXCR5−FOXP3+ T cells was positively correlated with HBV DNA levels, and longitudinal analysis demonstrated a high frequency of this subset at 12 weeks of antiviral treatment predicted unfavorable outcome in CHB patients.

Conclusions

CTLA4+CD4+CXCR5−FOXP3+ T cells are related to unfavorable outcomes in HBV-infected patients; these data indicated that alleviating CTLA4+CD4+CXCR5−FOXP3+ T cells may improve the prognosis of HBV infection.
Underlying mechanisms of long-term potentiation during the inhibition of the cannabinoid CB1 and GABAB receptors in the dentate gyrus of hippocampus
Authors: Nazari Masoumeh , Karimi Seyed Asaad , Komaki Somayeh , Kourosh Arami Masoumeh , Komaki Alireza
Published: January 2023

Background

The release of various neurotransmitters and thereby the excitability of neuronal circuits are regulated by the endocannabinoid system in an activity-dependent manner. Hippocampal long-term potentiation (LTP) is augmented in cannabinoid type 1 (CB1) receptor-deficient mice. CB1 receptors exist on GABAergic axon terminals in the hippocampus. In our previous work, we showed that CB1 antagonists increased the population spike (PS) amplitude, field excitatory post-synaptic potential (fEPSP), and the LTP induction in the dentate gyrus (DG) of the rat hippocampus while the GABAB antagonist decreased these parameters. Determining the underlying mechanisms of the pre- and/or postsynaptic locus of LTP expression is of great importance. In this study, we investigated whether LTP alteration acutely caused by CB1 and GABAB receptor antagonists (AM251 and CGP55845, respectively) happens at the postsynaptic or presynaptic regions, or at both. Therefore, the paired-pulse ratio (PPR) was assessed prior to and following the LTP induction in the studied groups.

Methods

Male Wistar rats were randomly assigned to the groups of control, AM251, CGP55845, CGP55845 + AM251. A high-frequency stimulation (HFS) of the perforant path (PP) was used to induce LTP in the DG region.

Results

Statistical analysis revealed that AM251 produced significant increase in excitatory postsynaptic potential (EPSP) slope and amplitude of PS. Conversely, administration of CGP55845 produced decrease in slope of EPSP. The current results indicated that the PPR was not influenced by LTP induction in the presence of AM251 or CGP55845 either alone or their combination.

Conclusions

It can be concluded that the site causing LTP expression is, at least in part, the postsynaptic site because PPR was not influenced by LTP induction in the presence of AM251 or CGP55845 either alone or their combination.
Proteomic analysis of protein lysine 2-hydroxyisobutyrylation (Khib) in soybean leaves
Authors: Zhao Wei , Ren Ting-Hu , Zhou Yan-Zheng , Liu Sheng-Bo , Huang Xin-Yang , Ning Tang-Yuan , Li Geng
Published: January 2023

Background

Protein lysine 2-hydroxyisobutyrylation (Khib) is a novel post-translational modification (PTM) discovered in cells or tissues of animals, microorganisms and plants in recent years. Proteome-wide identification of Khib-modified proteins has been performed in several plant species, suggesting that Khib-modified proteins are involved in a variety of biological processes and metabolic pathways. However, the protein Khib modification in soybean, a globally important legume crop that provides the rich source of plant protein and oil, remains unclear.

Results

In this study, the Khib-modified proteins in soybean leaves were identified for the first time using affinity enrichment and high-resolution mass spectrometry-based proteomic techniques, and a systematic bioinformatics analysis of these Khib-modified proteins was performed. Our results showed that a total of 4251 Khib sites in 1532 proteins were identified as overlapping in three replicates (the raw mass spectrometry data are available via ProteomeXchange with the identifier of PXD03650). These Khib-modified proteins are involved in a wide range of cellular processes, particularly enriched in biosynthesis, central carbon metabolism and photosynthesis, and are widely distributed in subcellular locations, mainly in chloroplasts, cytoplasm and nucleus. In addition, a total of 12 sequence motifs were extracted from all identified Khib peptides, and a basic amino acid residue (K), an acidic amino acid residue (E) and three aliphatic amino acid residues with small side chains (G/A/V) were found to be more preferred around the Khib site. Furthermore, 16 highly-connected clusters of Khib proteins were retrieved from the global PPI network, which suggest that Khib modifications tend to occur in proteins associated with specific functional clusters.

Conclusions

These findings suggest that Khib modification is an abundant and conserved PTM in soybean and that this modification may play an important role in regulating physiological processes in soybean leaves. The Khib proteomic data obtained in this study will help to further elucidate the regulatory mechanisms of Khib modification in soybean in the future.
Anaesthetic considerations in Shrinking Man syndrome: two case reports
Authors: Xu Yan , Hong Ying , Liu Xin , Zhou Li , Jiang Chunling
Published: January 2023

Background

Shrinking Man syndrome (SMS) is a rare but often serious complication of dialysis-dependent end-stage renal disease, characterized by significant loss of height, bone pain, bone deformity, and skin itching. Patients with SMS always have abnormal facial changes and cardiovascular system damage (manifested by hypertension, hypotension, cardiovascular calcification, and valvular heart disease), which pose a great challenge to anaesthesiologists. The purpose of this report is to describe our anaesthetic experience regarding two patients with SMS combined with alterations of the airway and cardiovascular system.

Case presentation

We describe two cases of SMS treated at West China Hospital, a tertiary care centre in Chengdu, China. All cases met the diagnostic criteria, which comprised 1) dialysis-dependent end-stage renal disease, 2) loss of height, and 3) bone pain and bone deformity. One patient had an anticipated difficult airway and moderate-to-severe mitral stenosis. The other patient presented with significant hypotension. Anaesthetic considerations included awake fibreoptic bronchoscopy-assisted tracheal intubation, real-time transoesophageal echocardiogram monitoring and individualized blood pressure management strategies.

Conclusion

This case series highlights the importance of adequate preoperative assessment and preparation, as well as individualized anaesthetic management, in patients with SMS.
Misspecification of confounder-exposure and confounder-outcome associations leads to bias in effect estimates
Authors: Schuster Noah A. , Rijnhart Judith J. M. , Bosman Lisa C. , Twisk Jos W. R. , Klausch Thomas , Heymans Martijn W.
Published: January 2023

Background

Confounding is a common issue in epidemiological research. Commonly used confounder-adjustment methods include multivariable regression analysis and propensity score methods. Although it is common practice to assess the linearity assumption for the exposure-outcome effect, most researchers do not assess linearity of the relationship between the confounder and the exposure and between the confounder and the outcome before adjusting for the confounder in the analysis. Failing to take the true non-linear functional form of the confounder-exposure and confounder-outcome associations into account may result in an under- or overestimation of the true exposure effect. Therefore, this paper aims to demonstrate the importance of assessing the linearity assumption for confounder-exposure and confounder-outcome associations and the importance of correctly specifying these associations when the linearity assumption is violated.

Methods

A Monte Carlo simulation study was used to assess and compare the performance of confounder-adjustment methods when the functional form of the confounder-exposure and confounder-outcome associations were misspecified (i.e., linearity was wrongly assumed) and correctly specified (i.e., linearity was rightly assumed) under multiple sample sizes. An empirical data example was used to illustrate that the misspecification of confounder-exposure and confounder-outcome associations leads to bias.

Results

The simulation study illustrated that the exposure effect estimate will be biased when for propensity score (PS) methods the confounder-exposure association is misspecified. For methods in which the outcome is regressed on the confounder or the PS, the exposure effect estimate will be biased if the confounder-outcome association is misspecified. In the empirical data example, correct specification of the confounder-exposure and confounder-outcome associations resulted in smaller exposure effect estimates.

Conclusion

When attempting to remove bias by adjusting for confounding, misspecification of the confounder-exposure and confounder-outcome associations might actually introduce bias. It is therefore important that researchers not only assess the linearity of the exposure-outcome effect, but also of the confounder-exposure or confounder-outcome associations depending on the confounder-adjustment method used.
Efficacy and safety of antibiotic therapy for post-Lyme disease? A systematic review and network meta-analysis
Authors: Zhang Xiaoqian , Jiang Yuwei , Chen Yihan , Yang Jiaru , Zhang Xiaoqi , Xing Lu , Liu Aihua , Bao Fukai
Published: January 2023

Background

At present, the pathogenesis of post-treatment Lyme disease (PTLDS) is not clear, so the treatment scheme of PTLDS, especially antibiotic treatment, is still controversial. This study aims to evaluate the efficacy of antibiotics in the treatment of PTLDS using network meta-analysis (NMA).

Methods

Following PRISMA guidelines, a systematic literature search was conducted on randomized controlled trials in PubMed, EMBASE, Web of Science and Cochrane Library (the literature was published from database inception through December 16, 2022). Using random effect model and fixed effect model. STATA17.0 software was used to evaluate the quality and heterogeneity of the included research literature.

Results

The system included 4 randomized controlled trials (485 subjects). The network meta-analysis showed that ceftriaxone had better results than placebo [Mean = 0.87, 95% CI (0.02, 1.71)] and doxycycline [Mean = 1.01, 95% CI (0.03, 1.98)] in FSS scale scores. There was no statistical difference in FSS scale scores of other drugs after treatment. In terms of FSS score results, Ceftriaxone was the best intervention according to the SUCRA value of each treatment (97.7). The analysis of outcome indicators such as Beck Depression Inventory (BDI), Mental-health Scale and Physical-functioning scale showed that there was no statistically significant difference between the antibiotic group and placebo group.

Conclusion

Ceftriaxone treatment may be the best choice for antibiotic treatment of PTLD, which provides useful guidance for antibiotic treatment of PTLD in the future.
Eculizumab as a treatment for C3 glomerulopathy: a single-center retrospective study
Authors: Welte Thomas , Arnold Frederic , Westermann Lukas , Rottmann Felix A. , Hug Martin J. , Neumann-Haefelin Elke , Ganner Athina
Published: January 2023

Background

C3 Glomerulopathy (C3G) is a rare glomerular disease caused by dysregulation of the complement pathway. Based on its pathophysiology, treatment with the monoclonal antibody eculizumab targeting complement C5 may be a therapeutic option. Due to the rarity of the disease, observational data on the clinical response to eculizumab treatment is scarce.

Methods

Fourteen patients (8 female, 57%) treated for C3 glomerulopathy at the medical center of the University of Freiburg between 2013 and 2022 were included. Subjects underwent biopsy before enrollment. Histopathology, clinical data, and response to eculizumab treatment were analyzed. Key parameters to determine the primary outcome were changes of estimated glomerular filtration rate (eGFR) over time. Positive outcome was defined as > 30% increase, stable outcome as ±30%, negative outcome as decrease > 30% of eGFR.

Results

Eleven patients (78.8%) were treated with eculizumab, three received standard of care (SoC, 27.2%). Median follow-up time was 68 months (IQR: 45–98 months).

Median eculizumab treatment duration was 10 months (IQR 5–46 months). After eculizumab treatment, five patients showed a stable outcome, six patients showed a negative outcome. Among patients receiving SoC, one patient showed a stable outcome, two patients showed a negative outcome.

Conclusions

The benefit of eculizumab in chronic progressive C3 glomerulopathy is limited.
Analysis of length of stay and treatment emergent complications in hospitalized myasthenia gravis patients with exacerbation
Authors: Ramsaroop Taylor , Gelinas Deborah , Kang Seung Ah , Govindarajan Raghav
Published: January 2023

Introduction

AIMS

Myasthenia Gravis (MG) is an autoimmune neuromuscular disease in which patients suffer from recurrent exacerbation. There are insufficient data measuring the effects of the resources employed before and during acute exacerbation on subsequent disease outcomes. This study aims to identify factors which lead to lengthened hospital stay.

Methods

This is a retrospective chart review of acute MG exacerbations requiring hospitalization. Exacerbations were identified using ICD-9/ICD-10 codes and considered the following variables: age and Myasthenia Gravis Foundation of America (MGFA) class at initial MG diagnosis, age and MGFA class at exacerbation, sex, thymectomy, cause of exacerbation, treatment regimen at time of exacerbation, inpatient treatment regimen, length of hospital stay (LOS), intubation, use of noninvasive ventilation, complications, and disposition.

Results

Seventy patients with 141 hospitalizations were identified. Crisis management characterized by intubation and plasmapheresis positively correlated with LOS (both p < .001). Almost 1/5 hospitalizations required intubation. Previous thymectomy negatively correlated with LOS (p < .05). In contrast, male sex correlated with longer LOS (p < .05). One-third of hospital stays were followed by discharge to a post-acute care facility, 7% home with home health, and 1 hospitalization resulted in death.

Discussion

Plasmapheresis, intubation, and male sex were associated with increased LOS in acute MG exacerbation. Intubation appears to be the strongest predictor of LOS. Those with previous thymectomy had shorter hospital stays. The role of thymectomy in the acute setting merits further analysis.
Activate & fire: a feasibility study in combining acoustic stimulation and continuous theta burst stimulation in chronic tinnitus
Authors: Schoisswohl Stefan , Langguth Berthold , Weber Franziska C. , Abdelnaim Mohamed A. , Hebel Tobias , Schecklmann Martin
Published: January 2023

Background

Low frequency repetitive transcranial magnetic stimulation (rTMS) is commonly used to inhibit pathological hyperactivity of the auditory cortex in tinnitus. Novel and supposedly superior and faster inhibitory protocols such as continuous theta burst stimulation (cTBS) were examined as well, but so far there is not sufficient evidence for a treatment application in chronic tinnitus. rTMS effects in general are dependent on the brain state immediate before stimulation. This feasibility study was designed based on the concept to shift the pathological intrinsic brain state of tinnitus patients via acoustic stimulation (“activate”) and induce inhibitory effects via cTBS (“fire”).

Methods

Seven tinnitus patients with response in residual inhibition received 10 consecutive daily sessions of a combinatory treatment comprised of 3-minute acoustic stimulation with white noise followed by 600 pulses of cTBS over the left temporo-parietal cortex (activate & fire). A control group of 5 patients was treated parallel to the activate & fire data collection with 10 sessions á 3000 pulses of 1 Hz rTMS over the left temporo-parietal cortex.

Results

The activate & fire protocol was well tolerated except in one patient with tinnitus loudness increase. This patient was excluded from analyses. No statistical superiority of the activate & fire treatment approach in alleviating tinnitus-related symptoms was evident. Power calculations showed an effect size of 0.706 and a needed sample size of 66 for statistical significant group differences. On a descriptive level the activate & fire group demonstrated a stronger decrease in tinnitus-related symptoms.

Conclusion

The present feasibility study showed that combining acoustic stimulation with magnetic brain stimulation may be well-tolerable in the majority of patients and represents a promising treatment approach for tinnitus by hypothetically alter the intrinsic state prior to brain stimulation.
A case of pituitary gland abscess associated with granulomatous hypophysitis
Authors: Rabiei Mohammad Mahdi , Ebrahimzadeh Kaveh , Davoudi Zahra , Bidari Zerehpoosh Farahnaz , Javandoust Gharehbagh Farid , Sedaghati Roghayeh , Lotfollahi Legha , Kalhor Fatemeh , Alavi Darazam Ilad
Published: January 2023

Background

Granulomatous hypophysitis is a rare disease that presents with chronic inflammation of the pituitary gland. In this study, we reported a case of granulomatous hypophysitis associated with a pituitary abscess.

Case presentation

A 39-year-old woman presented with a 2-year history of infertility. For the past six months, she has suffered from amenorrhea, decreased libido, headaches, and vertigo. She was referred to our hospital with a suspected diagnosis of nonfunctioning pituitary adenoma based on her presentation and brain MRI findings. She underwent trans-sphenoidal surgery (TSS). Direct observation during surgery revealed drainage of malodor pus and pituitary gland abscess. The histopathological evaluation also showed granulomatous hypophysitis and neutrophilic microabscess formation.

The patient was initially treated with high doses of ceftriaxone (2 g twice daily) and metronidazole (500 mg (mg) four times per day). Also, the patient received cortisol replacement therapy after the operation. After obtaining the antibiogram and culture results, the treatment regimen was continued for 4 weeks postoperatively, followed by amoxicillin-clavulanate (500/125 mg three times daily) for a total duration of 12 weeks.

Conclusion

The patient recovered uneventfully and the postoperative MRI was normal without any remnant lesions.
Prolonged pregnancy and stillbirth among women with overweight or obesity – a population-based study in Sweden including 64,632 women
Authors: Akselsson Anna , Rossen Jenny , Storck-Lindholm Elisabeth , Rådestad Ingela
Published: January 2023

Background

The proportion of overweight or obese pregnant women is increasing in many countries and babies born to a mother who is overweight or obese are at higher risk for complications. Our primary objective was to describe sociodemographic and obstetric factors across Body Mass Index (BMI) classifications, with secondary objective to investigate stillbirth and other pregnancy outcomes in relation to BMI classifications and gestational week.

Methods

This population-based cohort study with data partly based on a cluster-randomized controlled trial includes 64,632 women with singleton pregnancy, giving birth from 28 weeks’ gestation. The time period was January 2016 to 30 June 2018 (2.5 years). Women were divided into five groups according to BMI: below 18.5 underweight, 18.5–24.9 normal weight, 25.0–29.9 overweight, 30.0–34.9 obesity, 35.0 and above, severe obesity.

Results

Data was obtained for 61,800 women. Women who were overweight/obese/severely obese had lower educational levels, were to a lesser extent employed, were more often multiparas, tobacco users and had maternal diseases to a higher extent than women with normal weight. From 40 weeks’ gestation, overweight women had a double risk of stillbirth compared to women of normal weight (RR 2.06, CI 1.01–4.21); the risk increased to almost four times higher for obese women (RR 3.97, CI 1.6–9.7). Women who were obese or severely obese had a higher risk of almost all pregnancy outcomes, compared to women of normal weight, such as Apgar score < 7 at 5 min (RR1.54, CI 1.24–1.90), stillbirth (RR 2.16, CI 1.31–3.55), transfer to neonatal care (RR 1.38, CI 1.26–1.50), and instrumental delivery (RR 1.26, CI 1.21–1.31).

Conclusions

Women who were obese or severely obese had a higher risk of almost all adverse pregnancy outcomes and from gestational week 40, the risk of stillbirth was doubled. The findings indicate a need for national guidelines and individualized care to prevent and reduce negative pregnancy outcomes in overweight/obese women. Preventive methods including preconception care and public health policies are needed to reduce the number of women being overweight/obese when entering pregnancy.