bluuebunny/crossref_metadata_2025 huggingface dataset:
Strengths‐Based Assessment and Inclusive Language for Patients With Intellectual and Developmental Disabilities
Authors: Jain Shivani , Shah Karishma , Woo Seungmin , Dykman Morgan , Fung Lawrence K. , Rork Jillian F.
Published: December 2024

Pediatric dermatology patients with intellectual and developmental disabilities (IDD) and comorbid cutaneous conditions often face barriers to effective healthcare due to differences in communication preferences and sensitivities to environmental factors. The clinical intake process serves as a potential intervention point to help better understand and meet patients' needs. Strengths‐based assessment and considerations around identity‐first versus person‐first language are tools that can improve the clinical intake process in pediatric dermatology. We provide examples of intake questions and recommendations to help guide IDD‐informed care.
Global, Regional and National Burden of Maternal Obstructed Labour and Uterine Rupture, 1990–2021: Global Burden of Disease Study 2021
Authors: Guo Zhifeng , Ji Wangquan , Yan Mengqing , Shi Yang , Chen Teng , Bai Fanghui , Wu Yu , Guo Zhe , Song Linlin
Published: December 2024

Background

Maternal obstructed labour and uterine rupture (MOLUR) are among the major maternal labour complications that threaten maternal and child health.

Objective

The objective of this study was to systematically analyse the global burden of MOLUR using the Global Burden of Disease 2021 (GBD 2021) database to inform further improvements in maternal‐related public health policies.

Methods

Incidence and disability‐adjusted life‐year (DALY) data on MOLUR from 1990 to 2021 were collected in the GBD 2021. The joinpoint and Bayesian age–period–cohort models were used to analyse and predict time burden trends. The slope index and concentration index were used to evaluate health inequality. Frontier analysis was used to visualise the potential for burden reduction in individual countries or territories.

Results

In 2021, 13,471,093 (95% uncertainty interval [UI] 8,938,373, 19,008,282) incident cases of MOLUR were reported worldwide, which caused 1,067,270 (95% UI 896,161, 1,275,042) DALYs. Over the past three decades, there has been an overall downward trend in the age‐standardised incidence rate (ASIR) and age‐standardised DALY rate (ASDR) of MOLUR globally, with the ASIR decreasing from 554.0 (95% UI 355.6, 786.3) per 100,000 in 1990 to 347.0 (95% UI 228.8, 489.4) in 2021. The ASDR decreased from 65.4 (95% UI 56.0, 75.7) per 100,000 in 1990 to 27.0 (95% UI 22.7, 32.2) in 2021. By 2040, the global ASIRs and ASDRs projections for MOLUR will likely continue to decline. Socioeconomic‐related inequalities are narrowing, but the burden remains concentrated in low socioeconomically developed countries. Israel and Afghanistan showed the largest differences from the frontier boundaries of ASIR and ASDR.

Conclusions

Although the global burden of MOLUR has declined in the last three decades, it remains high and is still concentrated in economically underdeveloped countries. The reduction in DALYs attributable to MOLUR globally reflects significant progress in improving maternal health and reducing complications of childbirth.
The primacy of the practical: Russell Ford's Experience and Empiricism: Hegel, Hume, and the Early Deleuze
Authors: Lawlor Leonard
Published: December 2024

As the subtitle indicates, this article examines Russell Ford's new book on Deleuze's 1953 Empiricism and Subjectivity. Ford's book especially illuminates Deleuze's book on Hume in two ways. First, he shows how Deleuze's first book intervenes in an ongoing debate in French philosophy between transcendence and immanence. Second, Ford provides an intense reading of Deleuze's first book. The question, however, that Ford's book aims to answer is the nature of empiricism itself. My article reconstructs the precise definition of empiricism found in Experience and Empiricism. But also, because Ford stresses the priority of the practical in Deleuze's interpretation of Hume, my article, near the end, shows how Deleuze's view of the practical in his 1968 Difference and Repetition contests the view of the practical presented in Deleuze's Empiricism and Subjectivity.
A Decade in Transition: Forms of Recalibration in the Italian Regional Health Systems
Authors: Betti Marco , Maino Franca
Published: December 2024

Starting in the 1990s, healthcare legislation in Italy gradually expanded the powers and responsibilities of the regions. In this context, focusing solely on the National Health Service (NHS) may have limited our understanding of regional‐level transformations over the past decade. This article examines the restructuring of Italian Regional Health Services (SSR) from 2011 to 2021. We specifically analyse two key dimensions of healthcare: ordinary hospital beds and territorial structures, paying close attention to their composition and the public‐private nature of the offer. Our article aims to achieve two main objectives. Firstly, it describes the changes that have occurred in different SSRs over the past decade, deepening the functional, institutional and distributive aspects of ‘recalibration’. Secondly, we explore whether homogeneous clusters of SSRs emerged or if, on the contrary, the recalibration process has given rise to a kaleidoscope of health systems, underscoring the significant regional differentiation that has long characterised Italian healthcare.
Subsoiling with straw return promotes soil nitrogen supply and increase maize yield in saline‐alkaline farmland of the Yellow River Delta
Authors: Yu Chunxiao , Liu Hanwen , Dong Shide , Ma Qian , Zhang Haibo , Zhang Yi , Cui Guangxu , Yang Shihong , Wang Guangmei
Published: December 2024

The distinct soil physicochemical properties of saline‐alkali farmland often lead to nutrient deficiencies, particularly in nitrogen. Both straw return and tillage are essential practices for modifying saline‐alkali soils, however, their combined effects on soil properties, nitrogen(N) transformation and absorption, as well the crop yield remain inadequately understood. A field experiment was conducted to assess the cumulative impacts of straw return and tillage (rotary tillage, deep tillage and subsoiling) on soil salinity, nitrogen transformation and maize yield. The results revealed that the soil pH and electrical conductivity (EC) significantly decreased from the 0–20 cm layer to the 20–40 cm layer, indicating that deep tillage was less effective on salt accumulation in the surface soil. The return of straw return and tillage practices significantly influenced the fractions of soil organic nitrogen (SON) and the distribution percentage in total nitrogen. Additionally, these practices notably interacted to increase soil acid insoluble‐N, active SON and stable SON. The combination of straw return and rotary tillage resulted in a higher content of stable SON in the 0–20 cm soil layer. Conversely, the combination of straw return and subsoiling proved to be more effective on soil available N and active SON supplying, as well as maintaining stable nitrogen levels. Furthermore, straw return combined with subsoiling achieved the highest plant nitrogen uptake and yield, with increases of 21.7% and 38.2%, respectively, compared to the rotary without straw. Principal component analysis (PCA) revealed that stable SON was the primary contributor influencing maize yield, while inorganic N in the 0–20 cm layer and hydrolysable ammonium nitrogen (NH4+‐N) in the 20–40 cm were also the closely related factors. These results provide a new insight for implementing straw return measures to enhance soil quality and increase crop production in saline‐alkali farmland.
Phylogeny and classification of Cixiidae (Hemiptera, Fulgoromorpha): A new evolutionary scenario for the most diverse planthopper family
Authors: Luo Yang , Bucher Manon , Bourgoin Thierry , Löcker Birgit , Feng Ji‐Nian
Published: December 2024

The Cixiidae represent the most diverse family within Hemiptera Fulgoromorpha, accounting for nearly 20% of the described species. A molecular phylogenetic analysis of 147 taxa reveals a new evolutionary scenario for the family, identifying four major lineages: borystheninian (restricted to the Borysthenini), oecleinian and pentastirinian, grouped in one clade, sister to the cixiinian one. In the oecleinian lineage, the Oecleini are paraphyletic, including the Bothriocerini. Three groups are identified in the pentastirinian lineage: the Hyalesthes+, Pentastiridius+ and Oliarus+ clades. Within the cixiinian lineage, as traditionally recognised, the Cixiini tribe is polyphyletic, involving a basally separated Achaemenes clade, a newly described Chidaeini trib. nov., and the ‘true Cixiini’ clade, which itself remains paraphyletic, including the Semonini. The Andini tribe appears paraphyletic, including the Brixiini, and the position of the Gelastocephalini is yet to be confirmed. Despite its significance, the sampling remains incomplete, hindering, in our opinion, the formal taxonomic recognition of these lineages with formal ranks for a new classification of the Cixiidae. Fossil‐calibrated tree analysis indicates that Cixiidae originated in Lower Jurassic, approximately 181 million years ago. The four identified main lineages diverged during the Lower Jurassic in some 12 million years only, 155 million years ago. All currently recognised tribes and new major clades revealed with this study were present as early as the mid‐Cretaceous, around 100 million years ago; however, the Bennini tribe and the ‘true Cixiini’ clade emerged later, some 75 million years ago.
Phytosulfokine downregulates defense‐related WRKY transcription factors and attenuates pathogen‐associated molecular pattern‐triggered immunity
Authors: Liu Dian , Jelenska Joanna , Morgan Jessica M. , Greenberg Jean T.
Published: December 2024

Phytosulfokine (PSK) is a plant growth‐promoting peptide hormone that is perceived by its cell surface receptors PSKR1 and PSKR2 in Arabidopsis. Plants lacking the PSK receptors show phenotypes consistent with PSK signaling repressing some plant defenses. To gain further insight into the PSK signaling mechanism, comprehensive transcriptional profiling of Arabidopsis treated with PSK was performed, and the effects of PSK treatment on plant defense readouts were monitored. Our study indicates that PSK's major effect is to downregulate defense‐related genes; it has a more modest effect on the induction of growth‐related genes. WRKY transcription factors (TFs) emerged as key regulators of PSK‐responsive genes, sharing commonality with a pathogen‐associated molecular pattern (PAMP) responses, flagellin 22 (flg22), but exhibiting opposite regulatory directions. These PSK‐induced transcriptional changes were accompanied by biochemical and physiological changes that reduced PAMP responses, notably mitogen‐activated protein kinase (MPK) phosphorylation (previously implicated in WRKY activation) and the cell wall modification of callose deposition. Comparison with previous studies using other growth stimuli (the sulfated plant peptide containing sulfated tyrosine [PSY] and Pseudomonas simiae strain WCS417) also reveals WRKY TFs' overrepresentations in these pathways, suggesting a possible shared mechanism involving WRKY TFs for plant growth–defense trade‐off.
Restless legs syndrome among blood donors: A systematic review and meta‐analysis
Authors: Ngoma Alain M. , Mutombo Paulin B. , Mosli Mahmoud , Omokoko Magot D. , Nollet Kenneth E. , Ohto Hitoshi
Published: December 2024

Background and Objectives

Restless legs syndrome (RLS), with adverse health outcomes, has been linked to blood donation, but evidence published thus far has not been rigorously analysed. This systematic review aggregates existing evidence on RLS among blood donors and identifies associated factors worthy of further investigation.

Materials and Methods

MEDLINE and EMBASE were searched for articles published through 16 December 2023. Eleven studies from eight countries were selected from 142 publications. The pooled prevalence of RLS was calculated using a random‐effects model, with heterogeneity assessed by the Cochran Q and I2 statistics. Meta‐regression and sensitivity analyses explored sources of heterogeneity and the robustness of findings.

Results

Eleven studies, involving 20,255 blood donors, were included. The pooled prevalence of RLS among blood donors was 10.30% (95% confidence interval [CI]: 5.54%–16.30%), which was significantly higher than in the general adult population (3.0%, 95% CI: 1.4%–3.8%). Meta‐regression identified the year of study and geographical region as significant sources of heterogeneity. From the five studies that used logistic regression analyses, female sex and older age stand out as associated factors. No publication bias was detected, and sensitivity analysis confirmed the robustness of results.

Conclusion

Our findings suggest a high burden of RLS among blood donors, underscoring the need for further research with standardized criteria, appropriate design and analytical methodologies to better understand the impact of RLS on individual donors and the global blood supply.
Cluster Versus Rest-Redistribution Training: Similar Improvements in Neuromuscular Capacities in Female Team-Sport Athletes
Authors: Janicijevic Danica , Cuevas-Aburto Jesualdo , Jukic Ivan , Gu Yaodong , García-Ramos Amador
Published: December 2024

Purpose: This study’s purpose was to investigate the midterm effects of alternative set configurations (cluster [CL] and rest redistribution [RR]) on lower- and upper-body neuromuscular capacities in female athletes. Method: Twenty team-sport female athletes were randomly assigned to a CL (n = 10) or RR (n = 10) training group. The study protocol comprised 2 pretests, 12 training sessions, and a posttest. Both groups engaged in identical exercises (squat and bench press), load intensity (75% of 1-repetition maximum), and volume (18 repetitions per exercise). The distinction between the groups lay in the total session rest time: The CL group had 23 minutes (3 sets of 6 repetitions with 30 s of intraset rest every 2 repetitions and 3 min of interset rest), whereas the RR group had 17 minutes (9 sets of 2 repetitions with 45 s of interset rest). Countermovement-jump height and load–velocity relationship variables (load-intercept, velocity-intercept, and area under the load–velocity relationship line) were assessed during the squat and bench-press exercises. Results: All dependent variables revealed greater values at posttest compared with pretest (P ≤ .040; averaged Hedges g = 0.35 for CL and 0.60 for RR), but time × group interactions never reached statistical significance (P ≥ .144). Likewise, the comparison of the magnitude of changes between the 2 groups revealed only trivial differences, except for a small greater change in bench-press area under the load–velocity relationship line for RRG (Hedges g = 0.40). Conclusions: RR is a more efficient strategy than CL for inducing strength gains in female athletes.
Navigating a Path: Multidisciplinary Perspectives on Transitions in Sport Coaching
Authors: Dieffenbach Kristen , Blackett Alex , Rynne Steven , Chroni Stiliani “Ani”
Published: December 2024

This editorial for the Special Issue on Coaching Transitions in Sport highlights the importance of understanding the range of within-sport career transitions individuals experience while moving into and throughout their careers. Currently, the ways in which coaches adapt, evolve, and sustain their roles amid diverse and often complex career transitions have been largely unexamined. While other research has centered largely on topics such as coach effectiveness, stress, burnout, and well-being, this issue takes a multidisciplinary approach to broaden our understanding of coaching careers, focusing on transitions that occur over time and across contexts. Drawing on perspectives from psychology, sociology, pedagogy, and management, the manuscripts in this Special Issue provide much-needed insights into how coaching transitions are influenced by factors such as organizational support, interpersonal relationships, and cultural expectations. To grow this field of study, future research must examine diverse contexts beyond elite sport, incorporate experiences from marginalized groups, explore institutional and policy impacts, and consider the personal and systemic impacts of transitions. The cumulative impact of this Special Issue is intended to be the stimulation of a more comprehensive and supportive approach to coaching development, as well as continued interest in conducting research in this area.
Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging
Authors: Mizushima Kenta , Kumamoto Yasuaki , Tamura Shoko , Yamanaka Masahito , Mochizuki Kentaro , Li Menglu , Egoshi Syusuke , Dodo Kosuke , Harada Yoshinori , Smith Nicholas I. , Sodeoka Mikiko , Tanaka Hideo , Fujita Katsumasa
Published: December 2024

Raman microscopy is an emerging molecular imaging technology, yet its signal-to-noise ratio (SNR) in measurements of biological specimens is severely limited because of the small cross section of Raman scattering. Here, we present Raman imaging techniques of cryofixed specimens to overcome SNR limitations by enabling long exposure of specimens under highly stabilized low-temperature conditions. The observation of frozen specimens in a cryostat at a constant low temperature immediately after rapid freezing enabled the improvement of SNR and enhanced the spatial and spectral resolution. We also confirmed that the cryofixation can preserve physicochemical states of specimens by observing alkyne-labeled coenzyme Q in cytosol and hemeproteins in acute ischemic myocardium, which cannot be done by fixation using chemical reagents. Last, we applied the technique for multiplex Raman imaging of label-free endogenous molecules and alkyne-tagged molecules in cryofixed HeLa cells, demonstrating its capability of high-content imaging of complex biological phenomena while maintaining physiological conditions.
Repurposing an epithelial sodium channel inhibitor as a therapy for murine and human skin inflammation
Authors: Winge Mårten C. G. , Nasrallah Mazen , Jackrazi Leandra V. , Guo Konnie Q. , Fuhriman Jessica M. , Szafran Rebecca , Ramanathan Muthukumar , Gurevich Irina , Nguyen Ngon T. , Siprashvili Zurab , Inayathullah Mohammed , Rajadas Jayakumar , Porter Douglas F. , Khavari Paul A. , Butte Atul J. , Marinkovich M. Peter
Published: December 2024

Inflammatory skin disease is characterized by a pathologic interplay between skin cells and immunocytes and can result in disfiguring cutaneous lesions and systemic inflammation. Immunosuppression is commonly used to target the inflammatory component; however, these drugs are often expensive and associated with side effects. To identify previously unidentified targets, we carried out a nonbiased informatics screen to identify drug compounds with an inverse transcriptional signature to keratinocyte inflammatory signals. Using psoriasis, a prototypic inflammatory skin disease, as a model, we used pharmacologic, transcriptomic, and proteomic characterization to find that benzamil, the benzyl derivative of the US Food and Drug Administration–approved diuretic amiloride, effectively reversed keratinocyte-driven inflammatory signaling. Through three independent mouse models of skin inflammation (Rac1
G12V
transgenic mice, topical imiquimod, and human skin xenografts from patients with psoriasis), we found that benzamil disrupted pathogenic interactions between the small GTPase Rac1 and its adaptor NCK1. This reduced STAT3 and NF-κB signaling and downstream cytokine production in keratinocytes. Genetic knockdown of sodium channels or pharmacological inhibition by benzamil prevented excess Rac1-NCK1 binding and limited proinflammatory signaling pathway activation in patient-derived keratinocytes without systemic immunosuppression. Both systemic and topical applications of benzamil were efficacious, suggesting that it may be a potential therapeutic avenue for treating skin inflammation.
Comparison of the molecular FluoroType Mycobacteria VER 1.0 and the Maldi BioTyper Mycobacteria assays for the identification of non-tuberculous mycobacteria
Authors: Guiraud Jennifer , Piau Caroline , Enault Cécilia , Nkpa Charron Emilie , Ducos Danièle , Lafuente Christine , Ménard Armelle , Peuchant Olivia
Published: December 2024

Accurate identification of non-tuberculous mycobacterial (NTM) species is crucial for the diagnosis and appropriate management of NTM infections. This study aimed to evaluate the performance of two assays, FluoroType Mycobacteria VER 1.0 and Maldi BioTyper (MBT) Mycobacteria. The two assays were evaluated using 119 NTM, including 85 slow-growing mycobacteria and 34 rapid-growing mycobacteria, representing a total of 33 species isolated in three French clinical laboratories. We used the GenoType assays as reference method for species identification, followed by 16S rRNA gene sequencing if the GenoType kits returned
Mycobacterium
sp. Compared to the reference method, the FluoroType Mycobacteria assay provided correct species identification in 89.9% of cases (107/119). Among the most frequently encountered species in clinical settings, low concordance was obtained for
Mycobacterium intracellulare
(82.4%, 14/17),
Mycobacterium gordonae
(66.7%, 6/9), and
Mycobacterium xenopi
(75%, 6/8). Misidentification was obtained in two cases (
Mycobacterium smegmatis
instead of
Mycobacterium mageritense
, and
Mycobacterium mucogenicum
instead of
Mycobacterium phocaicum
). Using the MBT Mycobacteria assay, 78.1% (93/119) of NTM isolates were correctly identified at the species level. One
Mycobacterium europaeum
isolate was misidentified as
M. intracellulare
/
Mycobacterium chimaera
. In five cases, the assay provided more accurate NTM identification compared to GenoType assays, in which closely related species are identified as a group. The FluoroType Mycobacteria VER 1.0 and the MBT Mycobacteria assays are useful tools for NTM identification from positive cultures, reducing handling time compared to GenoType assays. Their routine use in laboratories must take into consideration their performance and limitations in clinical settings.
mSphere of Influence: How the single cell contributes to the collective
Authors: Lewin Gina R.
Published: December 2024

Gina Lewin works in the field of microbial ecology, with a focus on the human microbiota. In this mSphere of Influence article, she reflects on how two papers describing bacterial single-cell RNA-seq—“Prokaryotic single-cell RNA sequencing by
in situ
combinatorial indexing” by S. B. Blattman, W. Jiang, P. Oikonomou, and S. Tavazoie (Nat Microbiol 5:1192–1201, 2020,
https://doi.org/10.1038/s41564-020-0729-6
) and “Microbial single-cell RNA sequencing by split-pool barcoding” by A. Kuchina, L. M. Brettner, L. Paleologu, C. M. Roco, et al. (Science 371:eaba5257, 2021,
https://doi.org/10.1126/science.aba5257
)—impacted her work by developing a new approach to study how single cells of bacteria contribute to ecosystem-level processes.
Combined mutations in nonstructural protein 14, envelope, and membrane proteins mitigate the neuropathogenicity of SARS-CoV-2 Omicron BA.1 in K18-hACE2 mice
Authors: Sangare Kotou , Liu Shufeng , Selvaraj Prabhuanand , Stauft Charles B. , Starost Matthew F. , Wang Tony T.
Published: December 2024

We previously reported that mutations outside the spike protein play a role in the attenuation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron BA.1 variant in human ACE2 transgenic mice (K18-hACE2). Here, we assessed the pathogenicity of SARS-CoV-2 (WA1/2020) containing mutations from the Omicron BA.1 variant in K18-hACE2 mice. At an infection dose of 10
4
plaque-forming units (PFU), WA1 virus carrying Omicron BA.1 Nsp14(I42V), E(T9I), M(D3G/Q19E/A63T), but not Nsp6(Δ105–107, I189V), substitutions showed significant reduction in lethality. Interestingly, reduction of viral load is more pronounced in the brains than in the lungs. Subsequent analyses suggest that BA.1 E(T9I) and M(D3G/Q19E/A63T) substitutions result in less efficient packaging of virus-like particles. Given that Nsp14(I42V), E(T9I), M(Q19E/A63T) are well preserved in subsequent omicron subvariants, including currently circulating variants, our findings highlight the importance of understanding how non-spike mutations affect the pathogenicity of SARS-CoV-2 variants.

IMPORTANCE

Inoculation of transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) with SARS-CoV-2 often leads to a fatal brain infection. Omicron BA.1 variant, however, was found to be non-lethal in this model. Here, we systematically assessed the effect of individual mutations of Omicron BA.1 on the pathogenicity of the virus in hACE2 transgenic mice and found that combination of 5 mutations of Nsp14, E, and M of BA.1 variant significantly lowered brain viral load and reduced lethality. These results provide new insights into how SARS-CoV-2 Omicron BA.1 is attenuated.
Development and validation of genome-informed and multigene-based qPCR and LAMP assays for accurate detection of
Dickeya solani
: a critical quarantine pathogen threatening the potato industry
Authors: Dobhal Shefali , Santillana Gem , Stulberg Michael J. , Arizala Dario , Alvarez Anne M. , Arif Mohammad
Published: December 2024

Dickeya solani
one of the most aggressive pectinolytic phytopathogens, causes blackleg disease in potatoes, resulting in significant economic losses and adversely impacting one of the world’s most important food crops. The diagnostics methods are critical in monitoring the latent infection for international trade of potato seed tubers and in implementation of control strategies. Our study employed a whole-genome comparative approach, identifying unique target gene loci (LysR and TetR family of transcriptional regulators gene regions) and designing loop-mediated isothermal amplification (LAMP) and a multi-gene-based multiplex TaqMan qPCR assays for specific detection and differentiation of
D. solani
. Both methods underwent meticulous validation with extensive inclusivity and exclusivity panels, exhibiting 100% accuracy and no false positives or negatives. The LAMP method demonstrated the detection limit of 100 fg and 1 CFU per reaction using pure genomic DNA and crude bacterial cell lysate, respectively. The qPCR detection limit was 1 pg, 100 fg and 10 fg with quadplex, triplex, and singleplex, respectively. None of the assays were impacted by any inhibitory or competitive effects after adding host DNA (in qPCR) or crude lysate (in LAMP). The assays proved robust and reproducible in detecting the target pathogen in infected samples, with the LAMP assay being field-deployable due to its simplicity and rapid results acquisition within approximately 9 min. The reproducibility was confirmed by performing the assay in two independent laboratories. These assays offer a robust, rapid, and reliable solution for routine testing, with applications in phytosanitary inspection, disease diagnosis, and epidemiological studies.

IMPORTANCE

Dickeya solani
, one of the most aggressive soft rot causing bacteria and a quarantine pathogen, poses a severe threat to food security by causing substantial economic losses to the potato industry. Accurate and timely detection of this bacterium is vital for monitoring latent infections, particularly for international trade of potato seed tubers, and for implementing effective control strategies. In this research, we have developed LAMP and multi-gene-based multiplex TaqMan qPCR assays for specific detection of
D. solani
. These assays, characterized by their precision, rapidity, and robustness, are crucial for distinguishing
D. solani
from related species. Offering unparalleled sensitivity and specificity, these assays are indispensable for phytosanitary inspection and epidemiological monitoring, providing a powerful tool enabling management of this threatening pathogen.
Erratum to: Automated Synthesis of [N-Methyl-11C]choline, Radiopharmaceutical for Tumor Imaging by PET
Authors: Vaulina D. D. , Kuznetsova O. F. , Orlovskaya V. V. , Fedorova O. S. , Krasikova R. N.
Published: December 2024

Erratum
Erratum to: Nanodiamonds as Lutetium-177 Carriers for Nuclear Medicine
Authors: Kazakov A. G. , Babenya J. S. , Ekatova T. Y. , Vinokurov S. E. , Khvorostinin E. Y. , Ushakov I. A. , Zukau V. V. , Stasyuk E. S. , Nesterov E. A. , Sadkin V. L. , Rogov A. S. , Myasoedov B. F.
Published: December 2024

Erratum
Erratum to: Magneto-Biological Physics
Authors: Buchachenko A. L.
Published: December 2024

An Erratum to this paper has been published: https://doi.org/10.1134/S1990793124340025
Erratum to: Statistical Analysis of Flux Enhancements of Energetic Electrons in the Low-Latitudinal Ionosphere According to the Data from the NOAA/POES and MetOp Satellites from 1998 to 2022
Authors: Golubkov M. G. , Suvorova A. V. , Dmitriev A. V. , Golubkov G. V.
Published: December 2024

An Erratum to this paper has been published: https://doi.org/10.1134/S1990793124340050
Babinski-Nageotte syndrome after vertebral artery dissection and foramen arcuale
Authors: Marques Ana João , Azoia Carolina , Rocha Luís , Almendra Ricardo
Published: December 2024

Vertebrobasilar ischaemia happens for a myriad of reasons. Among the rarest is the existence of a foramen arcuale (FA). This condition results from the ossification of the lateral edge of the posterior atlantooccipital membrane, closing, completely or partially, the top of this groove, turning it into a foramen or a channel. The estimated prevalence of this anatomical variant is around 9% for the complete form. It is postulated that entrapment of vertebral artery can occur. We share the case of a man in his 30s presenting with sudden imbalance, vertigo, vomiting and hiccups. Neurological observation disclosed Babinski-Nageotte syndrome. CT scan revealed left vertebral artery (VA) signs of dissection at its third segment (VA3), occlusion of its ipsilateral fourth segment (VA4) and bilateral FA. MRI confirmed ischaemic lesions in vertebrobasilar territory. The patient underwent surgical excision of contralateral FA. No new neurological adverse events occurred after 2 years of follow-up.
MANIPULATION CORROSION RESISTANCE AND SUPER-HYDROPHOBICITY OF LASER SURFACE TEXTURED AISI 316 STAINLESS STEEL DECORATED WITH HEXADECYLTRIMETHOXYSILANE (HDTMS)
Authors: LIU LIN , ZHANG LUXIA , LIN NAIMING , YUAN SHUO , YANG YANG , LIU ZHIQI , ZENG QUNFENG , NOURI MEISAM
Published: December 2024

Super-hydrophobicity inspired by natural water-repellent surfaces has received tremendous attention for surface functionalization of metallic materials in many applications. Problems of corrosion and/or contamination that happens on the surface can be partially solved as the material obtained super-hydrophobicity. Appropriate surface pattern and low surface energy are the two key factors that can realize super-hydrophobicity. In the present paper, laser surface texturing (LST) followed by decoration of Hexadecyltrimethoxysilane (HDTMS) was conducted to manipulate the super-hydrophobicity and corrosion resistance of AISI 316 stainless steel (SS) on the surface. Scanning electron microscopy (SEM) and an ultra-depth field microscope were employed to characterize the surface morphologies of the received samples before and after corrosion tests. Corrosion resistance and super-hydrophobicity for the related samples were investigated using electrochemical tests and water contact angle measurements. It was found that various surface patterns differed with intervals (250, 150, 80, and [Formula: see text]m,) between two micro-dimples have formed on 316 SS sample surfaces after LST treatment. The surface roughness values gradually increased with the decrease of interval. The 316 SS samples obviously reduced surface energy values after HDTMS decoration. In simulated haze solution, electrochemical tests demonstrated that the 316 SS sample treated with [Formula: see text] (HT-316 SS, [Formula: see text]m) exhibited lower corrosion and higher corrosion potential current density than the smooth 316 SS (S-316 SS), [Formula: see text]-316 SS (HS-316 SS), and LST 316 SS (T-316 SS, [Formula: see text]m) samples. As expected, the HT-316 SS ([Formula: see text]m) exhibited the highest water contact angle of 155.85∘ as compared to HS-316 SS and other HT-316 SS samples. Meanwhile, the superhydrophobic surface of HT-316 SS ([Formula: see text]m) exhibits super-oleophilic and self-cleaning properties.
Investigation of infertility treatments on infertile couples through fractional-order modeling approach
Authors: Yeolekar Mahesh A. , Yeolekar Bijal M. , Khirsariya Sagar R. , Chauhan Jignesh P.
Published: December 2024

In this paper, our aim is to construct a nonlinear mathematical model to study the impact of treatment on the infertility problem. We utilize a well-known Caputo derivative with fractional-order to decrease the issue of infertility treatments. Banach fixed-point theory is used to determine the existence and uniqueness of the suggested model. Equilibrium points have been calculated by generating reproduction numbers as a part of local stability analysis, and Ulam–Hyers–Rassias stability is discussed as a generalized form of system to prove global stability. We use a fractional-order Euler’s method to determine the numerical and graphical results. The result manifests the improvement of achieving a healthy pregnancy through hormonal and IVF treatment in women.
About this title - Structure and Evolution of Laurussian Orogens in Europe and North America from Geophysical Investigations
Authors: Ben-Mansour W. , Schiffer C. , Gradmann S.
Published: December 2024

Two main Paleozoic continental collisions leading to the assembly of the palaeocontinent Laurussia, reshaped the surface topography and lithosphere in large areas of present-day Europe and North America. This volume presents recent advances in our understanding of the structure and evolution of these orogens from different geophysical methods and perspectives.
Neural Circuits of Fear and Anxiety: Insights from a Neuroethological Perspective
Authors: Melleu Fernando Falkenburguer , Canteras Newton Sabino
Published: December 2024

The predatory imminence continuum (PIC) of antipredator defensive behavior has been a helpful strategy for modeling anxiety and fear-related disorders in nonclinical research. The PIC is divided into three different sequential stages that reflect defensive behavioral strategy in response to predatory imminence. However, the PIC was experimentally addressed using a series of shock-based fear conditioning experiments rather than predatory threats. In this study, we will consider the PIC in a more naturalistic behavioral setting, focusing on analyzing the neural systems of animals responding to terrestrial and aerial predators. Of relevance, there is a sequential engagement of the distinct neural circuits along each phase of the PIC. In the preencounter phase, prefrontal cortical networks are particularly involved in planning and organizing behavioral responses to ambiguous threats. As the predatory cues or the real predator is detected, there is an engagement of amygdalar and hippocampal > hypothalamic pathways in conjunction with the periaqueductal gray, which organize fear responses. This dynamic particularly reveals how specific neural circuits are set into action to subserve distinct defensive responses. Moreover, we further explored the neural circuits governing other fearful situations outside the context of the PIC, including agonistic social encounters and interoceptive challenges. This analysis revealed an interesting overlap between the neural systems responding to these threats and those involved in response to predatory threats. The present review clarifies how defensive circuits respond to natural threats and provides a more realistic view of the neural systems underlying anxiety and fear responses.
Antidiabetic Effect of Acalypha hispida Extract and Nanoextract: Mechanism of Action on Pancreatic β‐Cell Function via Histone Acetylation
Authors: Alfarisi Hamzah , Sa’diah Siti , Juliandi Berry , Wresdiyati Tutik
Published: December 2024

The global prevalence of diabetes mellitus was 537 million in 2021 and is projected to increase to 783 million by 2045. Diabetes progression is also associated with histone modifications. Previous studies have reported that Acalypha hispida leaves are rich in flavonoids and phenolic acids, which are associated with antihyperglycemic activity. Recently, we produced a nanoextract of A. hispida leaf that contains bioactive compounds with antidiabetic effects. However, there were no studies reported the antidiabetic efficacy of A. hispida extract and nanoextract. Hence, we aimed to evaluate the antidiabetic effects of A. hispida extract and nanoextract on pancreatic β‐cells, insulin secretion, glycogen content, and histone modifications in streptozotocin‐induced diabetic rats. Diabetic rats received oral doses of the extract (300 mg/kg bw) and nanoextract (30 and 60 mg/kg bw) daily for 28 days. Bodyweight, blood glucose levels, insulin levels, islets of Langerhans and pancreatic β‐cells, glycogen content, acetylation of lysine 9 on histone 3 (H3K9Ac) level, hematology, and lipid profile were assessed. Analyses of variance, principal component analysis (PCA), and partial least squares were performed. The nanoextract of A. hispida was successfully produced at a nanosize of 733 nm. The extract (300 mg/kg bw) and nanoextract (60 mg/kg bw) exhibited antidiabetic effects based on the following parameters: decreased blood glucose levels, increased pancreatic β‐cell number and density, increased insulin levels, decreased H3K9Ac level in the islets of Langerhans and liver, enhanced glycogen levels in the liver, and improved lipid metabolism. Moreover, PCA indicated that the nanoextract (60 mg/kg bw) showed the strongest antidiabetic effect based on these parameters. The increased β‐cell number and density led to decreased blood glucose levels. In conclusion,​ A. hispida extract and nanoextract have antidiabetic effects by restoring β‐cell number and density and decreasing H3K9 acetylation in the islets of Langerhans in streptozotocin‐induced diabetic rats.
Diagnostic Performance of Dermoscopy and Clinical Visual Diagnosis for Plantar Warts
Authors: León-Herce Diego , García-Oreja Sara , Navarro-Pérez David , Tardáguila-García Aroa , Lázaro-Martínez José Luis , Álvaro-Afonso Francisco Javier
Published: December 2024

Background: Plantar warts caused by the human papilloma virus (HPV) are one of the most frequent pathologies in podiatry. Diagnosis is usually limited to the clinical presentation of the lesion. Biopsy and polymerase chain reaction (PCR) are expensive and can be difficult to access. However, the dermatoscope is a noninvasive tool that covers the gap between microscopic and macroscopic diagnosis.

Objective: This study compares the effectiveness of diagnosis of plantar warts using a dermatoscope versus visual clinical signs.

Methods: The study evaluated 25 patients with suspected HPV plantar warts by visual or dermatoscopic signs. Upon clinical suspicion of HPV, a sample was taken for PCR analysis. A dermatoscopic image of the plantar wart was collected, and the characteristic clinical signs were evaluated, including the discontinuity of dermatoglyphs, hemorrhagic dots, reddish linear vessels, verruciform surface and frog‐spawn appearance.

Results: All 25 patients showed positive results in molecular testing. Dermatoscopic findings compatible with HPV were obtained for 100% (25/25) of patients, while clinical signs were observed in 84% (21/25). The most common finding was the alteration of dermatoglyphs, which was present in all patients at the dermoscopic level. The sensitivity of the dermatoscope was 100% and identical to that of PCR.

Conclusion: The dermatoscope appears to be a useful, noninvasive and rapid tool for clinical use in the diagnosis of plantar warts.
The Role of Ozonated Jerusalem Artichoke Ointment on the Healing of Surgically Created Full‐Thickness Cutaneous Wounds in Rabbits
Authors: Atiyah Ali Ghazi , Hasan Mustafa Salah , Owain Maher Saber
Published: December 2024

Jerusalem artichoke (J.A.) tubers contain compounds that exhibit anti‐inflammatory effects and can minimize tissue damage. Ozone is an alternative antimicrobial and immunomodulatory agent for promoting tissue regeneration. The present study aimed to evaluate the therapeutic effect of the ozonated J.A. ointment on a surgically created full‐thickness cutaneous wound in rabbit models. The previously prepared J.A. ointment was ozonated using a Herrmann generator, followed by a subsequent evaluation of its physical and antibacterial properties. Thirty healthy male albino rabbits were used in this study. The animals were divided into two equal groups: the control and treated group. An excisional wound model was used to assess wound healing activities. All of the animals underwent surgical preparation of their dorsal surfaces, and excisional lesions of 3 cm in diameter were created on each animal’s dorsal surface of the thoracolumbar region. In the control group, the wounds were left untreated. The animals in the treatment group received a topical application of ozonated J.A. ointment twice daily for five days following the injury. The animals were euthanized on Days 7, 14, and 21 after the injury for histological evaluation. The agar well diffusion method demonstrated the antimicrobial efficacy of the ozonated J.A. ointment. Also, macroscopic and histopathological results showed a significant (p < 0.05) increase in wound area contraction with enhancement re‐epithelization in the treated group compared to the control group. In conclusion, the ozonated ointment derived from J.A. tubers has antibacterial properties and can promote and enhance the wound healing process.
Supplementary Data from Mathematical Model-Driven Deep Learning Enables Personalized Adaptive Therapy
Authors: Gallagher Kit , Strobl Maximilian A.R. , Park Derek S. , Spoendlin Fabian C. , Gatenby Robert A. , Maini Philip K. , Anderson Alexander R.A.
Published: December 2024

Supplementary Information File
Cytogenomic Characterization of Murine Osteosarcoma Cell Line SEWA
Authors: Liehr Thomas , Rincic Martina
Published: December 2024

Introduction: The SEWA cell line, which is derived from a virus-induced murine osteosarcoma (OS) ascites, was established in the 1980s from a serially transplanted male-derived tumor that was first published in 1961. It has been applied in about 50 studies but was never genetically characterized in detail; this study fills that gap. Methods: The SEWA cell line was analyzed for its chromosomal constitution using molecular cytogenetic approaches. Array comparative genomic hybridization was performed to characterize copy number alterations. Results: SEWA has a near-diploid karyotype without Y-chromosome material. The complex karyotype includes neocentrics and simple and complex rearrangements. Amplification of MYC oncogene was detected in two homogeneously staining regions on two different derivative chromosomes. Conclusion: An in silico translation of the obtained results to the human genome indicated that SEWA is suitable as a model for advanced human OS.
The Effect of Dietary Phosphate Load on Urinary Supersaturation and Phosphate Metablism in Non-stone-forming Asian Individuals
Authors: Li Xuezhu , Zhang Weichen , Huang Tao , Chen Yingjie , Li Jiaolun , Ding Feng , Wang Wenji
Published: December 2024

Introduction Phosphate ion is common in the core of urinary stones and may initiate stone formation. However, the precise role of phosphate in the initiation of stone formation remains obscure. We assessed the effects of dietary phosphate load on urinary stone risk and phosphate metabolism.
Methods Ten non-stone-forming healthy volunteers completed this randomized, crossover study. Each subject was provided a high or low phosphate diet for 3 days. After a 2-day equilibration period with a moderate phosphate diet, the participants received a low or high phosphate diet for another 3 days. Serum, fecal and 24-hour urine samples were collected at the end of each intervention.
Results High dietary phosphate load increased urinary phosphate excretion, and low dietary phosphate decreased urinary phosphate excretion. In addition, urine pH was lower and supersaturation index of uric acid was higher after high a phosphate diet. There was no significant difference in the supersaturation index for calcium oxalate, brushite and hydroxyapatite, or in 24-hour urinary citrate between the high and the low dietary phosphate. Also, no significant change was observed in fecal phosphate excretion after a high or low phosphate diet. The serum phosphate level increased after a high phosphate diet, but there was no difference in serum phosphate between low phosphate and moderate phosphate diets.
Conclusion High dietary phosphate load led to higher urinary phosphate excretion, a higher supersaturation index of uric acid, and lower urine pH. Stone formers should be advised to limit the intake of high phosphate source diet, including high protein diets and phosphate-based food additives.
Diagnostic Imaging of Primary Cutaneous Osteoma of the Forehead
Authors: Nakaso Soma , Ishii Nobuaki , Akimoto Masataka , Ogawa Rei
Published: December 2024

Introduction: Osteoma cutis is a rare benign condition characterized by ectopic bone formation in the dermis or subcutaneous tissue. Typically, osteoma cutes result from inflammation, tumors, or scars. However, primary cases occasionally present with no apparent preceding lesion. Case Presentation: we report a case of primary osteoma cutis located on a 20-year-old woman's forehead. Diagnostic imaging revealed a nodule of bone tissue within the dermis and contiguous cartilage tissue, which was later histologically confirmed. The patient underwent surgical resection under local anesthesia and successfully recovered with no neurological symptoms or signs of recurrence 6 months after the surgery. Conclusion: We report this case because, to the best of our knowledge, there have been no reports of primary osteoma cutis with complete preoperative imaging findings.
Participación de niñas, niños y adolescentes institucionalizados: perspectivas durante la emergencia sociosanitaria
Authors: Zúñiga Daniela , Mardones Rodolfo , Wentzel Viether Marianne , Sanhueza José
Published: December 2024

(analytical)This work explores the perspectives of directors, educators, children and adolescents institutionalized under protection measures, regarding the principle of participation and the right to be heard in the context of a health emergency. The study has a qualitative approach, instrumental single case design. 21 focused interviews were carried out in 4 residences in the Los Ríos Region, Chile, and their contents were analyzed. The perspectives about the right to be heard and participation are linked to the organizational characteristics of the residences; highly structuring, precarious and with high responsibility in the care of children and adolescents. Although the rights are known and valued by the actors, the application is difficult since it challenges building a sensitive and flexible relationship with children and adolescents in an adverse residential context.
Keywords:  Youth participation; institutionalization; pandemic; child welfare; child rights; governmental policy.
Da aculturação à formação: o papel dos estudos sobre a imigração na obra de Florestan Fernandes
Authors: Fernandes Júnior João Gilberto Belvel
Published: December 2024

Having received little attention from the interpreters of Florestan Fernandes’ work until recently, the author’s migratory studies,  although revisited by specialists in this field, have been the object of implicit or explicit accusations of blame for the poor development of the theme in sociology, and especially at Escola Sociológica Paulista, from  the 1940s onwards. This work aims to respond to this “sociology of abandonment” in the  interpretation of Fernandes’ work, showing, through a systematic interpretation, that the author’s supposed departure from migratory studies did not happen without the provisions of an accumulative movement, characteristic of the formative model that the author fought for.