bluuebunny/crossref_metadata_2025 huggingface dataset:
Coordinated State Analysis and Differential Regulation of Territorial Spatial Functions in Underdeveloped Regions: A Case Study of Gansu Province, China
Authors: Xie Baopeng , Wang Quanxi , Huang Beiying , Chen Ying , Yang Jie , Qi Peixin
Published: January 2022

The coordinated development of territorial spatial functions is the main manifestation of the sustainable utilization of territorial resources. Identifying spatial functions and their coordinated relationship has become an important guarantee for regional coordinated development, and is of great significance to the construction of a sustainable land development and protection pattern. This study constructs a classification and function scoring system for Production-Living-Ecological Spaces (PLES) by using land-use data from Gansu Province in 2000 and 2020, and revises the spatial functions of the land to supplement the deficiencies of existing related researches by combining socio-economic data (GDP, population density) and ecosystem service value data. In the aforementioned works, the Mechanical Equilibrium Model in physics is referenced to explore the coordinated state among territorial spatial functions. The results show that the high-value areas of production function are mainly distributed in the eastern and central areas of the Loess Plateau, with a strip-like distribution in the Hexi Corridor. The high-value areas of living function are consistent with that in the center of the city. The distribution of high-value areas of ecological function is in line with the topographic distribution pattern of mountainous areas in Gansu Province. The distribution pattern of the coordinated state of land space function in Gansu Province is relatively stable from 2000 to 2020. Simultaneously, the functional dominant area and the promotion area are identified according to the deviation of the coordinated degree of production-living-ecological function. After clarifying the functional characteristics of each county, the author proposes a differentiated regulation strategy of territorial spatial function.
Exploring the Energy Saving Potential in Private, Public and Non-Motorized Transport for Ten Swedish Cities
Authors: Kenworthy Jeffrey R. , Svensson Helena
Published: January 2022

Transport energy conservation research in urban transport systems dates back principally to the Organization of the Petroleum Exporting Countries’ (OPEC) “Arab Oil Embargo” (1973–1974) and the Iranian revolution (1979), when global oil supplies became threatened and costs rose steeply. Two subsequent Gulf Wars (1991 and 2003) highlighted the dangerous geo-political dimensions of Middle-Eastern oil. In latter times, the urgency to reduce global CO2 output to avoid catastrophic climate change has achieved great prominence. How to reduce passenger transport energy use therefore remains an important goal, which this paper pursues in ten Swedish cities, based on five scenarios: (1) increasing the relatively low public transport (PT) seat occupancy in each Swedish city to average European levels (buses 35%, light rail 48%, metro 60% and suburban rail 35%); (2) doubling existing PT seat occupancy in each Swedish city; (3) increasing existing car occupancy in each Swedish city by 10%; (4) decreasing existing energy use per car vehicle kilometer by 15%; (5) increasing existing modal split for daily trips by non-motorized modes to 50% in each city. A sixth “best-case scenario” is also explored by simultaneously combining scenarios 2 to 5. The data used in the paper come from systematic empirical research on each of the ten Swedish cities. When applied individually, scenario 2 is the most successful for reducing passenger transport energy use, scenarios 1 and 4 are next in magnitude and produce approximately equal energy savings, followed by scenario 5, with scenario 3 being the least successful. The best-case, combined scenario could save 1183 million liters of gasoline equivalent in the ten cities, representing almost a 60% saving over their existing 2015 total private passenger transport energy use and equivalent to the combined 2015 total annual private transport energy use of Stockholm, Malmö and Jönköping. Such findings also have important positive implications for the de-carbonization of cities. The policy implications of these findings and the strategies for increasing public transport, walking and cycling, boosting car occupancy and decreasing vehicular fuel consumption in Swedish cities are discussed.
Operating Successfully on a New Technological Path: The Effect of External Search
Authors: Stucki Tobias , Woerter Martin
Published: January 2022

Switching to a new technological path is often a serious economic challenge for companies. Incumbents, in particular, are often led by their organizational routines, traditional technological orientation, and experience, and run the risk of losing contact with new technologies, which can decrease their competitiveness. We analyze whether opening up the innovation process to external knowledge partners can help to overcome such path dependence and enable firms to operate successfully on a new technological path. We develop a theoretical concept that shows the potential of external knowledge sources for operating successfully on a new technological path and test it empirically using the example of green technologies. Green technologies are not only relevant for addressing the current environmental problems, but they are also an example of a new technological path that is proving difficult for companies to switch to. Overall, we find strong direct effects of external (green) knowledge on green innovation success. The results even indicate that the direct effect of external knowledge tends to be larger for green than for non-green innovation.
Sustainable Streetscapes: Design Approaches and Examples of Viennese Practice
Authors: Furchtlehner Jürgen , Lehner Daniela , Lička Lilli
Published: January 2022

Streets are ubiquitous and cater to various functions in a city. However, today most streets are unilaterally used and designed likewise. Car-centred spatial distribution is currently being questioned in the course of urban densification and in light of climate and ecological challenges. The presented work focuses on a multi-layered transformation of streetscapes towards a multi-purpose social and ecological space, which goes beyond a mere redistribution of space and functions. This paper draws from the results of an interdisciplinary research project headed by the Institute of Landscape Architecture (University of Natural Resources and Life Sciences, Vienna). The Viennese situation is aligned with international trends. The research includes comparative analysis of streetscapes in Vienna and comparable cities, literature reviews, collaborative workshops and qualitative interviews. As a result, progressive layout specifications and quality aspects for future streets are proposed and presented in extracts. Furthermore, the goal of green space social equity is linked. The paper concludes by arguing for comprehensive consideration and redesign of streetscapes as one promising puzzle to counteract the evident challenges of climate change in urban settings. Its range reaches from small scale microclimatic improvements up to citywide provision of accessible, useable, ecologically sound and sustainable public space with new standards for streets as potential backbone.
Circularity in Practice: Review of Main Current Approaches and Strategic Propositions for an Efficient Circular Economy of Materials
Authors: Megevand Benjamin , Cao Wen-Jun , Di Maio Francesco , Rem Peter
Published: January 2022

This paper aims to summarize, propose, and discuss existing or emerging strategies to shift towards a circular economy of materials. To clarify the landscape of existing circular practices, a new spectrum is proposed, from product-based strategies, where entire products go through several life cycles without being reprocessed, to material-based approaches, extracting, recycling, and reprocessing materials from the waste flow. As refillable packaging does not lose any functionality or value, when re-used through many life cycles, product-based strategies are globally extremely efficient and must be promoted. It appears however that their implementation is only possible at the scale of individual products such as packaging containers, relying on the cooperation of involved companies and consumers. It appears more and more urgent to focus as well on a more systematic and flexible material-oriented scheme. The example of circular glass recycling is a success in many countries, and technologies become nowadays available to extend such practices to many other materials, such as rigid plastics. An ideal would be to aim at an economy of materials that would imitate the continuous material cycle of the biosphere. Technological and business strategies are presented and discussed, aiming at a relevant impact on circularity.
Indoor Air Quality in Healthcare Units—A Systematic Literature Review Focusing Recent Research
Authors: Fonseca Ana , Abreu Isabel , Guerreiro Maria João , Barros Nelson
Published: January 2022

The adequate assessment and management of indoor air quality in healthcare facilities is of utmost importance for patient safety and occupational health purposes. This study aims to identify the recent trends of research on the topic through a systematic literature review following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) methodology. A total of 171 articles published in the period 2015–2020 were selected and analyzed. Results show that there is a worldwide growing research interest in this subject, dispersed in a wide variety of scientific journals. A textometric analysis using the IRaMuTeQ software revealed four clusters of topics in the sampled articles: physicochemical pollutants, design and management of infrastructures, environmental control measures, and microbiological contamination. The studies focus mainly on hospital facilities, but there is also research interest in primary care centers and dental clinics. The majority of the analyzed articles (85%) report experimental data, with the most frequently measured parameters being related to environmental quality (temperature and relative humidity), microbiological load, CO2 and particulate matter. Non-compliance with the WHO guidelines for indoor air quality is frequently reported. This study provides an overview of the recent literature on this topic, identifying promising lines of research to improve indoor air quality in healthcare facilities.
Characteristic of Stomatal Conductance and Optimal Stomatal Behaviour in an Arid Oasis of Northwestern China
Authors: Han Tuo , Feng Qi , Yu Tengfei , Yang Xiaomei , Zhang Xiaofang , Li Kuan
Published: January 2022

Stomatal conductance (gs), the process that governs plant carbon uptake and water loss, is fundamental to most Land Surface Models (LSMs). With global change accelerating, more attention should be paid to investigating stomatal behavior, especially in extremely arid areas. In this study, gas exchange measurements and environmental/biological variables observations during growing seasons in 2016 and 2017 were combined to investigate diurnal and seasonal characteristics of gs and the applicability of the optimal stomatal conductance model in a desert oasis vineyard. The results showed that the responses of gs to environmental factors (photosynthesis active radiation, PAR; vapor pressure deficit, VPD; and temperature, T) formed hysteresis loops in the daytime. The stomatal conductance slope, g1, a parameter in the unified stomatal optimal model, varied in different growing seasons and correlated with the soil-to-leaf hydraulic conductance (KL). These results indicated the potential bias when using a constant g1 value to simulate gs and highlighted that the water-use strategy of oasis plants might not be consistent throughout the entire growing season. Our findings further help to achieve a better understanding of stomata behavior in responding to climate change and encourage future efforts toward a more accurate parameterization of gs to improve the modeling of LSMs.
Sustainability and Agricultural Regeneration in Hungarian Agriculture
Authors: Kovách Imre , Megyesi Boldizsár Gergely , Bai Attila , Balogh Péter
Published: January 2022

Generational renewal is a core issue in European agriculture. Despite the continuous efforts of governments and the EU Council, the ageing of farmers seems an unstoppable process, accompanied by land concentration, the decrease in agricultural activity and the transformation of the European countryside. Consequently, there is a very rich scientific literature analysing the problem; a great part of it argues that the young farmer problem consists, in fact, in a number of different problems, with these problems showing huge regional differences. Hungary, as a new member state, with a heterogeneous (both fragmented and concentrated) land-use structure offers a good field to analyse generational renewal. Our paper is based on the first results of an ongoing Horizon 2020 project analysing rural regeneration. As a part of the research study, 48 semi-structured interviews were conducted with young farmers, successors of farmers and new entrants into farming. In our paper, we explore how education, access to land and family traditions influenced generational renewal and how it impacts sustainability practices.
Analytical Comparison of German Owner’s Budget Estimate and Chinese Contractor’s Bid: A Case Study for a Warehouse Building Project in China
Authors: Li Gen , Xu Rong
Published: January 2022

Nowadays, the need for scientific and modernized engineering cost management has been increasingly prominent. Hence, this study compared the differences of the quantities’ terms, the management philosophy, the related institutions (or specifications), and other factors in engineering cost management between China and Germany accompanied by a case study for a warehouse building project in China. There are two main parts. Firstly, according to the literature research, the comparative analysis was conducted on the engineering cost-control mode, the list of bills of quantities, and other parameters between China and Germany. On the basis of this, the “differences” between China and Germany especially in the bidding process were further studied with the empirical analysis of engineering cases, which is a supplementary argument to the theoretical analysis. Finally, several related suggestions were put forward, including proposing a set of “universal” bills of quantities (which can be understood by Chinese enterprises and also accepted by foreign-funded enterprises), the establishment of the whole process, a dynamic and information-based project cost-management system, cultivation of talents with the advanced management concept, and the development of international engineering companies and project-management companies with core competitiveness. The conclusions are expected to provide some references for China’s construction industry to further expand the international market and to increase the influences in global markets.
Consumers’ Willingness to Buy CRISPR Gene-Edited Tomatoes: Evidence from a Choice Experiment Case Study in Germany
Authors: Götz Linde , Svanidze Miranda , Tissier Alain , Brand Duran Alejandro
Published: January 2022

The CRISPR gene-editing (GE) breeding method is used to increase the resilience of high-yielding tomato cultivars against pests and diseases, reducing crop protection requirements. This study investigated consumers’ willingness to buy CRISPR GE tomatoes in a repeated discrete-choice experiment. We observed a strong positive effect of providing information on the CRISPR breeding technology, while the sensory experience of the CRISPR GE tomatoes in a visit to a greenhouse had a rather weak, predominantly negative effect on the participants’ willingness to buy CRISPR GE tomatoes. We found that roughly half of the 32 participants demonstrated constant CRISPR GE tomato choices during the experiments, and these participants were mainly employed as scientists. However, the rest of the participants changed their CRISPR GE tomato choices, with the majority showing an increase in their willingness to buy CRISPR GE tomatoes; these “changers” were dominated by non-scientists. Science communication on CRISPR GE breeding technology should target people with little knowledge about the technology, and consumers of organic tomatoes seem to have more specified, stable preferences regarding the technology. Further, scientific information about the CRISPR GE methodology should preferentially be provided when new technology and information about it are not yet widespread and people have not yet formed a strong opinion about the technology.
Food Neophobia among Brazilian Children: Prevalence and Questionnaire Score Development
Authors: de Almeida Priscila Claudino , Vasconcelos Ivana Aragão Lira , Zandonadi Renata Puppin , Nakano Eduardo Yoshio , Raposo António , Han Heesup , Araya-Castillo Luis , Ariza-Montes Antonio , Botelho Raquel Braz Assunção
Published: January 2022

This study aims to create and validate a score to classify food neophobia among Brazilian children (from the ages of 4 to 11 years) and investigate the prevalence of food neophobia. This descriptive cross-sectional population-based study is conducted following three steps: (i) the application of an instrument to identify food neophobia in Brazilian children by their caregivers; (ii) the instrument’s score definition; and (iii) the evaluation and characterization of the national prevalence of food neophobia among Brazilian children. The scores were categorized into three levels, based on the tertial approximation: low, moderate, and high. The study had 1112 participants, and the prevalence of high food neophobia was observed in 33.4% of Brazilian children. The prevalence of food neophobia allowed us to identify this behavior in Brazilian children and better understand the population. Boys were significantly more neophobic than girls. The general neophobia score and domains did not significantly differ between Brazilian regions and age groups. It is worrying that food neophobia did not decrease with advancing age. The score for the complete instrument with 25 items, or the 3 domains, makes its use practical. It can be used to assess neophobia with more caution, evaluate the most neophobic children, and enable more targeted professional interventions to promote healthier and sustainable eating habits.
External Two Stage DEA Analysis of Bank Efficiency in West Balkan Countries
Authors: Milenković Nada , Radovanov Boris , Kalaš Branimir , Horvat Aleksandra Marcikić
Published: January 2022

Since the beginning of the application of the Data Envelopment Analysis (DEA) model in various areas of the economy, it has found its wide application in the field of finance, more specifically banks, in the last few years. The focus of this research was to determine the sustainability of the intermediate function of banks, especially in recent years when interest rates on deposits have been at a minimum level. The research was divided into two parts, wherein the first part determined the efficiency of the intermediate function of banks in the countries of the Western Balkans in the period from 2015 to 2019. The second part approached the regression analysis in which we determined the influence of the bank size, type of bank, and mergers and acquisitions (M&A) activity on the defined efficiency. In the first stage we applied the output-oriented DEA model using deposits, labor costs, and capital as input variables; on the other side, we used loans and investments as output variables. We used data from the revised financial statements of the banks operating in Serbia, Bosnia and Herzegovina, Montenegro, North Macedonia, and Albania. The results of our study showed that there is a difference in efficiency levels between countries and within countries in the considered time period. Furthermore, Tobit regression analysis showed a significant and negative influence of the bank type and M&A on relative technical efficiency of banks, and a positive and significant relationship between bank size and relative efficiency. These findings suggest that large commercial banks can sustain on the West Balkan market. It is to be expected that less efficient small banks will be taken over by large and more efficient banks.
Durability Assessment of ETICS: Comparative Evaluation of Different Insulating Materials
Authors: Landolfi Roberto , Nicolella Maurizio
Published: January 2022

The External Thermal Insulation Composite System (ETICS) is a common cladding technology that is widely used thanks to its well-known advantages. Despite previous studies dealing with ETICS durability in real-building case studies or involving accelerated ageing tests in climatic chambers, little progress has been made in the knowledge of the long-term durability of the system. In order to realize optimized maintenance plans for this component, the durability of the whole system, and of the most-used insulating materials for the ETICS (i.e., cork, polyurethane, rock wool, glass wool, grey EPS, and fiberfill wood), has been investigated. Based on previous experiments on ageing cycles, different climatic chambers were used to accelerate performance decay by simulating natural outdoor exposure in order to assess different physical and thermal characteristics (thermal transmittance, decrement factor, time shift, water absorption, thermal resistance, and conductivity). Recorded trends show that materials with lower thermal conductivity exhibit lower performance decay, and vice versa. The durability of the ETICS with different insulating materials (as the only variable in the different samples) was evaluated in order to quantify service life and then correctly plan maintenance interventions. Life-cycle assessment must take into account service life and durability for each material of the system. A higher durability of insulating materials allows for the execution of less maintenance interventions, with the loss of less performance over time. This study shows the physical and thermal behavior of the ETICS during its service life, comparing the differences induced by the most-used insulating materials. As a result of accelerated ageing cycles, the analyzed ETICS reveals a low grade of decay and measured performances show little degradation; for thermal conductivity, differences between the measured and the declared conductivities by technical datasheet were observed.
Investigating the Impact of International Markets and New Digital Technologies on Business Innovation in Emerging Markets
Authors: Dana Léo-Paul , Salamzadeh Aidin , Mortazavi Samira , Hadizadeh Morteza
Published: January 2022

International markets and digital technologies are considered among the factors affecting business innovation. The emergence and deployment of digital technologies in emerging markets increase the innovation potential in businesses. Companies with an entrepreneurial orientation also strengthen their innovation capabilities. The present study aimed to investigate the impact of international markets and new digital technologies on business innovation in emerging markets, and to estimate the mediating effect of entrepreneurial orientation on this relationship. The present research was applied research in terms of aim and descriptive survey in terms of data collection method and quantitative in terms of the type of collected data. A standard questionnaire was to collect data. The study’s statistical population consisted of all companies providing business services in Tehran, Iran. To analyse the data, the structural equation modelling method with partial least squares method and Smart PLS-3 Software was used. The results revealed that international markets and digital technologies are positively associated with innovation. They also revealed that when a company’s entrepreneurial orientation increases, the digital technologies and international markets will be more involved in mutual relationships.
Nutrient Flows and Balances in Mixed Farming Systems in Madagascar
Authors: Fanjaniaina Marie Lucia , Stark Fabien , Ramarovahoaka Noelly Phostin , Rakotoharinaivo Jeanny Fiononana , Rafolisy Tovonarivo , Salgado Paulo , Becquer Thierry
Published: January 2022

Mixed farming systems are still prevalent in sub-Saharan Africa. In these systems, the recycling of nutrients through crop-livestock integration (CLI) practices is crucial for the sustainability of soil fertility and crop production. The objective of this study was to analyze nutrient (N, P, K) flows and balances of mixed farming systems to assess CLI contribution to the performance of those systems. We hypothesized that more intensive farms had a better nutrient balance at the farm level, and that improved biomass management methods improved their nutrient balance. Nine farms in the Madagascar highlands were selected, some corresponding to poor traditional farms with only draft cattle; some small or medium-sized, more intensive farms with a dairy herd; and some of the latter with some improvement to management methods of livestock effluents (manure composting, liquid manure collection). The nutrient balance of the farming systems was determined, and performance indicators were calculated at both farming, livestock, and CLI levels. Results showed that nutrient recycling through CLI is significant in the functioning of the systems studied, contributing primarily to circulating nutrient flows (up to 76%) and leading to greater efficiency and productivity. Nutrient flows resulting from these practices mainly concerned animal feeding (higher than 60% of nutrient flows), even if manure management was central for crop fertilization and that manure remained a desired animal product of these types of farms (up to 100% of animal products). Large negative balances of N and K (up to 80% of inputs) were observed in traditional livestock systems with draft cattle. They were smaller (39–68%) in more intensive dairy farms. Composting of manure did not decrease negative balances, whereas their magnitude was significantly reduced by the collection of liquid manure (19% for N; 42% for K). Better management of biomass at the farm level, in particular the collection of liquid manure, seemed to substantially reduce nutrient losses in MFS.
Friction Characteristics Analysis of Symmetric Aluminum Alloy Parts in Warm Forming Process
Authors: Xia Jiansheng , Zhao Jun , Dou Shasha
Published: January 2022

There are many typical symmetric large plastic deformation problems in aluminum alloy stamping. Warm stamping technology can improve the formability of materials and obtain parts with high-dimensional accuracy. Friction behavior in the stamping process is significant for the forming quality. An accurate friction coefficient is helpful in improving the prediction accuracy of forming defects. It is hard to obtain a unified and precise friction model through simple experiments due to the complicated contact conditions. To explore the effect of friction behavior on the forming quality, warm friction experiments of the AA6061 aluminum alloy and P20 steel with different process parameters were carried out using a high-temperature friction tester CFT-I (Equipment Type), including temperatures, the interface load, and sliding speeds. The variation curves of the friction coefficient with various parameters were obtained and analyzed. The results show that the friction coefficient increases with temperature and decreases with the sliding speed and load. Then, the influences of process parameters on the surface morphology of the samples after friction were observed by an optical microscope; adhesive wear occurred when the temperature increased, and the surface scratch increased and deepened with the increase in the load. Finally, the friction coefficient models of the speed and load were established by analyzing the data with Original software. Compared with the experimental and the finite element analysis results of the symmetrical part, the errors of the velocity friction model in thickness and springback angle are less than 4% and 5%, respectively. The mistakes of the load friction model are less than 6% and 7%, respectively. The accuracy of the two friction models is higher than that of the constant friction coefficient. Therefore, those coefficient models can effectively improve the simulation accuracy of finite element software.
Hybrid Analysis of the Decision-Making Factors for Software Upgrade Based on the Integration of AHP and DEMATEL
Authors: Kim Dosung , Kim Mi
Published: January 2022

Software is a very important part to implement advanced information systems, such as AI and IoT based on the latest hardware equipment of the fourth Industrial Revolution. In particular, decision making for software upgrade is one of the essential processes that can solve problems for upgrading the information systems. However, most of the decision-making studies for this purpose have been conducted only from the perspective of the IT professional and management position. Moreover, software upgrade can be influenced by various layers of decision makers, so further research is needed. Therefore, it is necessary to conduct research on what factors are required and affect the decision making of software upgrade at various layers of organization. For this purpose, decision factors of software upgrade are identified by literature review in this study. Additionally, the priority, degree of influence and relationship between the factors are analyzed by using the AHP and DEMATEL techniques at the organizational level of users, managers and IT professionals. The results show that the priority, weight value, causal relationship of decision factors of users, managers and IT professionals who constitute the organizational level were very different. The managers first considered the benefits, such as ROI, for organization as a leader. The users tended to consider their work efficiency and changes due to the software upgrade first. Finally, the IT professionals considered ROI, budget and compatibility for the aspect of the managers and users. Therefore, the related information of each organizational level can be presented more clearly for the systematic and symmetrical decision making of software upgrade based on the results of this study.
Assistive Technologies for Supporting the Wellbeing of Older Adults
Authors: Dratsiou Ioanna , Varella Annita , Romanopoulou Evangelia , Villacañas Oscar , Cooper Sara , Isaris Pavlos , Serras Manex , Unzueta Luis , Silva Tatiana , Zurkuhlen Alexia , MacLachlan Malcolm , Bamidis Panagiotis D.
Published: January 2022

As people age, they are more likely to develop multiple chronic diseases and experience a decline in some of their physical and cognitive functions, leading to the decrease in their ability to live independently. Innovative technology-based interventions tailored to older adults’ functional levels and focused on healthy lifestyles are considered imperative. This work proposed a framework of active and healthy ageing through the integration of a broad spectrum of digital solutions into an open Pan-European technological platform in the context of the SHAPES project, an EU-funded innovation action. In conclusion, the SHAPES project can potentially engage older adults in a holistic technological ecosystem and, therefore, facilitate the maintenance of a high-quality standard of life.
Snakebite Associated Thrombotic Microangiopathy and Recommendations for Clinical Practice
Authors: Noutsos Tina , Currie Bart J. , Wijewickrama Eranga S. , Isbister Geoffrey K.
Published: January 2022

Snakebite is a significant and under-resourced global public health issue. Snake venoms cause a variety of potentially fatal clinical toxin syndromes, including venom-induced consumption coagulopathy (VICC) which is associated with major haemorrhage. A subset of patients with VICC develop a thrombotic microangiopathy (TMA). This article reviews recent evidence regarding snakebite-associated TMA and its epidemiology, diagnosis, outcomes, and effectiveness of interventions including antivenom and therapeutic plasma-exchange. Snakebite-associated TMA presents with microangiopathic haemolytic anaemia (evidenced by schistocytes on the blood film), thrombocytopenia in almost all cases, and a spectrum of acute kidney injury (AKI). A proportion of patients require dialysis, most survive and achieve dialysis free survival. There is no evidence that antivenom prevents TMA specifically, but early antivenom remains the mainstay of treatment for snake envenoming. There is no evidence for therapeutic plasma-exchange being effective. We propose diagnostic criteria for snakebite-associated TMA as anaemia with >1.0% schistocytes on blood film examination, together with absolute thrombocytopenia (<150 × 109/L) or a relative decrease in platelet count of >25% from baseline. Patients are at risk of long-term chronic kidney disease and long term follow up is recommended.
Characterization of the Composition and Biological Activity of the Venom from Vespa bicolor Fabricius, a Wasp from South China
Authors: Wu Yong-Hua , Zhang Yu , Fang Dan-Qiao , Chen Jing , Wang Jing-An , Jiang Lin , Lv Zhu-Fen
Published: January 2022

We analyzed, for the first time, the major components and biological properties of the venom of Vespa bicolor, a wasp from South China. Using HPLC and SDS-PAGE, combined with LC–MS/MS, MALDI-TOF-MS, and NMR data to analyze V. bicolor venom (VBV), we found that VBV contains three proteins (hyaluronidase A, phospholipase A1 (two isoforms), and antigen 5 protein) with allergenic activity, two unreported proteins (proteins 5 and 6), and two active substances with large quantities (mastoparan-like peptide 12a (Vb-MLP 12a), and 5-hydroxytryptamine (5-HT)). In addition, the antimicrobial activity of VBV was determined, and results showed that it had a significant effect against anaerobic bacteria. The minimum inhibitory concentration and minimum bactericidal concentration for Propionibacterium acnes were 12.5 µg/mL. Unsurprisingly, VBV had strong antioxidant activity because of the abundance of 5-HT. Contrary to other Vespa venom, VBV showed significant anti-inflammatory activity, even at low concentrations (1 µg/mL), and we found that Vb-MLP 12a showed pro-inflammatory activity by promoting the proliferation of RAW 264.7 cells. Cytotoxicity studies showed that VBV had similar antiproliferative effects against all tested tumor cell lines (HepG2, Hela, MCF-7, A549, and SASJ-1), with HepG2 being the most susceptible. Overall, this study on VBV has high clinical importance and promotes the development of Vespa bicolor resources.
Estimating the Cosmological Constant from Shadows of Kerr–de Sitter Black Holes
Authors: Afrin Misba , Ghosh Sushant G.
Published: January 2022

The Event Horizon Telescope collaboration has revealed the first direct image of a black hole, as per the shadow of a Kerr black hole of general relativity. However, other Kerr-like rotating black holes of modified gravity theories cannot be ignored, and they are essential as they offer an arena in which these theories can be tested through astrophysical observation. This motivates us to investigate asymptotically de Sitter rotating black holes wherein interpreting the cosmological constant Λ as the vacuum energy leads to a deformation in the vicinity of a black hole—new Kerr–de Sitter solution, which has a richer geometric structure than the original one. We derive an analytical formula necessary for the shadow of the new Kerr–de Sitter black holes and then visualize the shadow of black holes for various parameters for an observer at given coordinates (r0,θ0) in the domain (r0,rc) and estimate the cosmological constant Λ from its shadow observables. The shadow observables of the new Kerr–de Sitter black holes significantly deviate from the corresponding observables of the Kerr–de Sitter black hole over an appreciable range of the parameter space. Interestingly, we find a finite parameter space for (Λ, a) where the observables of the two black holes are indistinguishable.
Extending the Coding Potential of Viral Genomes with Overlapping Antisense ORFs: A Case for the De Novo Creation of the Gene Encoding the Antisense Protein ASP of HIV-1
Authors: Pavesi Angelo , Romerio Fabio
Published: January 2022

Gene overprinting occurs when point mutations within a genomic region with an existing coding sequence create a new one in another reading frame. This process is quite frequent in viral genomes either to maximize the amount of information that they encode or in response to strong selective pressure. The most frequent scenario involves two different reading frames in the same DNA strand (sense overlap). Much less frequent are cases of overlapping genes that are encoded on opposite DNA strands (antisense overlap). One such example is the antisense ORF, asp in the minus strand of the HIV-1 genome overlapping the env gene. The asp gene is highly conserved in pandemic HIV-1 strains of group M, and it is absent in non-pandemic HIV-1 groups, HIV-2, and lentiviruses infecting non-human primates, suggesting that the ~190-amino acid protein that is expressed from this gene (ASP) may play a role in virus spread. While the function of ASP in the virus life cycle remains to be elucidated, mounting evidence from several research groups indicates that ASP is expressed in vivo. There are two alternative hypotheses that could be envisioned to explain the origin of the asp ORF. On one hand, asp may have originally been present in the ancestor of contemporary lentiviruses, and subsequently lost in all descendants except for most HIV-1 strains of group M due to selective advantage. Alternatively, the asp ORF may have originated very recently with the emergence of group M HIV-1 strains from SIVcpz. Here, we used a combination of computational and statistical approaches to study the genomic region of env in primate lentiviruses to shed light on the origin, structure, and sequence evolution of the asp ORF. The results emerging from our studies support the hypothesis of a recent de novo addition of the antisense ORF to the HIV-1 genome through a process that entailed progressive removal of existing internal stop codons from SIV strains to HIV-1 strains of group M, and fine tuning of the codon sequence in env that reduced the chances of new stop codons occurring in asp. Altogether, the study supports the notion that the HIV-1 asp gene encodes an accessory protein, providing a selective advantage to the virus.
Detection of Porcine Respirovirus 1 (PRV1) in Poland: Incidence of Co-Infections with Influenza A Virus (IAV) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in Herds with a Respiratory Disease
Authors: Woźniak Aleksandra , Cybulski Piotr , Denes Lilla , Balka Gyula , Stadejek Tomasz
Published: January 2022

Porcine respirovirus 1 (PRV1) is also known as porcine parainfluenza virus 1 (PPIV1). The prevalence and the role of PRV1 infections for pig health is largely unknown. In order to assess the PRV1 prevalence in Poland, nasal swabs and oral fluids collected from pigs from 30 farms were examined with RT real-time PCR. Additionally, IAV and PRRSV infection statuses of PRV1-positive samples were examined. The results showed that the virus is highly prevalent (76.7% farms positive) and different patterns of PRV1 circulation in herds with mild–moderate respiratory disease were observed. Co-infections with IAV and PRRSV were infrequent and detected in 8 (23.5%) and 4 (11.8%) out of 34 PRV1-positive nasal swab pools from diseased pens, respectively. In one pen PRV1, IAV, and PRRSV were detected at the same time. Interestingly, PRV1 mean Ct value in samples with co-infections was significantly lower (29.8 ± 3.1) than in samples with a single PRV1 infection (32.5 ± 3.6) (p < 0.05), which suggested higher virus replication in these populations. On the other hand, the virus detection in pig populations exhibiting respiratory clinical signs, negative for PRRSV and IAV, suggests that PRV1 should be involved in differential diagnosis of respiratory problems.
HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread
Authors: Kalinichenko Svetlana , Komkov Dmitriy , Mazurov Dmitriy
Published: January 2022

So far, only two retroviruses, human immunodeficiency virus (HIV) (type 1 and 2) and human T-cell lymphotropic virus type 1 (HTLV-1), have been recognized as pathogenic for humans. Both viruses mainly infect CD4+ T lymphocytes. HIV replication induces the apoptosis of CD4 lymphocytes, leading to the development of acquired immunodeficiency syndrome (AIDS). After a long clinical latency period, HTLV-1 can transform lymphocytes, with subsequent uncontrolled proliferation and the manifestation of a disease called adult T-cell leukemia (ATLL). Certain infected patients develop neurological autoimmune disorder called HTLV-1-associated myelopathy, also known as tropical spastic paraparesis (HAM/TSP). Both viruses are transmitted between individuals via blood transfusion, tissue/organ transplantation, breastfeeding, and sexual intercourse. Within the host, these viruses can spread utilizing either cell-free or cell-to-cell modes of transmission. In this review, we discuss the mechanisms and importance of each mode of transmission for the biology of HIV-1 and HTLV-1.
Actin Contributes to the Hyperexpression of Baculovirus Polyhedrin (polh) and p10 as a Component of Transcription Initiation Complex (TIC)
Authors: Chen Nan , Chen Guanping , Kong Xiangshuo , Wu Xiaofeng
Published: January 2022

Hyperexpression of polh and p10, two very late genes, is one of the remarkable characteristics in the baculovirus life cycle. However, the mechanisms underlying the hyperexpression of these two genes are still incompletely understood. In this study, actin was identified as a highly potential binding partner of polh and p10 promoters by conducting DNA pull-down and LC–MS/MS analyses. Inhibiting actin dynamics delayed and decreased the transcription of polh and p10. Actin interacted with viral RNA polymerase and transcription regulators, and the nuclear import of viral polymerase was inhibited with the disruption of actin dynamics. Simultaneously, the high enrichment of actin in polh and p10 promoters discovered via a chromatin immunoprecipitation (ChIP) assay indicated that actin was a component of the viral polymerase TIC. Moreover, overexpression of actin surprisingly upregulated the expression of luciferase (Luc) under the control of polh and p10 promoters. Taken together, actin participated in the hyperexpression of polh and p10 as a component of TIC. These results facilitate the promotion of the expression efficiency of foreign genes in the baculovirus expression vector system (BEVS).
Bayesian Inference of State-Level COVID-19 Basic Reproduction Numbers across the United States
Authors: Mallela Abhishek , Neumann Jacob , Miller Ely F. , Chen Ye , Posner Richard G. , Lin Yen Ting , Hlavacek William S.
Published: January 2022

Although many persons in the United States have acquired immunity to COVID-19, either through vaccination or infection with SARS-CoV-2, COVID-19 will pose an ongoing threat to non-immune persons so long as disease transmission continues. We can estimate when sustained disease transmission will end in a population by calculating the population-specific basic reproduction number ℛ0, the expected number of secondary cases generated by an infected person in the absence of any interventions. The value of ℛ0 relates to a herd immunity threshold (HIT), which is given by 1−1/ℛ0. When the immune fraction of a population exceeds this threshold, sustained disease transmission becomes exponentially unlikely (barring mutations allowing SARS-CoV-2 to escape immunity). Here, we report state-level ℛ0 estimates obtained using Bayesian inference. Maximum a posteriori estimates range from 7.1 for New Jersey to 2.3 for Wyoming, indicating that disease transmission varies considerably across states and that reaching herd immunity will be more difficult in some states than others. ℛ0 estimates were obtained from compartmental models via the next-generation matrix approach after each model was parameterized using regional daily confirmed case reports of COVID-19 from 21 January 2020 to 21 June 2020. Our ℛ0 estimates characterize the infectiousness of ancestral strains, but they can be used to determine HITs for a distinct, currently dominant circulating strain, such as SARS-CoV-2 variant Delta (lineage B.1.617.2), if the relative infectiousness of the strain can be ascertained. On the basis of Delta-adjusted HITs, vaccination data, and seroprevalence survey data, we found that no state had achieved herd immunity as of 20 September 2021.
Emerging Socioeconomic Disparities in COVID-19 Vaccine Second-Dose Completion Rates in the United States
Authors: Gertz Autumn , Rader Benjamin , Sewalk Kara , Brownstein John S.
Published: January 2022

Although COVID-19 vaccination plans acknowledge a need for equity, disparities in two-dose vaccine initiation have been observed in the United States. We aim to assess if disparity patterns are emerging in COVID-19 vaccination completion. We gathered (n = 843,985) responses between February and November 2021 from a web survey. Individuals self-reported demographics and COVID-19 vaccination status. Dose initiation and completion rates were calculated incorporating survey weights. A multi-variate logistic regression assessed the association between income and completing vaccination, accounting for other demographics. Overall, 57.4% initiated COVID-19 vaccination, with 84.5% completing vaccination. Initiation varied by income, and we observed disparities in completion by occupation, race, age, and insurance. Accounting for demographics, higher incomes are more likely to complete vaccination than lower incomes. We observe disparities in completion across annual income. Differences in COVID-19 vaccination completion may lead to two tiers of protection in the population, with certain sub-groups being better protected from future infection.
Harnessing Mycobacterium bovis BCG Trained Immunity to Control Human and Bovine Babesiosis
Authors: Bastos Reginaldo G. , Alzan Heba F. , Rathinasamy Vignesh A. , Cooke Brian M. , Dellagostin Odir A. , Barletta Raúl G. , Suarez Carlos E.
Published: January 2022

Babesiosis is a disease caused by tickborne hemoprotozoan apicomplexan parasites of the genus Babesia that negatively impacts public health and food security worldwide. Development of effective and sustainable vaccines against babesiosis is currently hindered in part by the absence of definitive host correlates of protection. Despite that, studies in Babesia microti and Babesia bovis, major causative agents of human and bovine babesiosis, respectively, suggest that early activation of innate immune responses is crucial for vertebrates to survive acute infection. Trained immunity (TI) is defined as the development of memory in vertebrate innate immune cells, allowing more efficient responses to subsequent specific and non-specific challenges. Considering that Mycobacterium bovis bacillus Calmette-Guerin (BCG), a widely used anti-tuberculosis attenuated vaccine, induces strong TI pro-inflammatory responses, we hypothesize that BCG TI may protect vertebrates against acute babesiosis. This premise is supported by early investigations demonstrating that BCG inoculation protects mice against experimental B. microti infection and recent observations that BCG vaccination decreases the severity of malaria in children infected with Plasmodium falciparum, a Babesia-related parasite. We also discuss the potential use of TI in conjunction with recombinant BCG vaccines expressing Babesia immunogens. In conclusion, by concentrating on human and bovine babesiosis, herein we intend to raise awareness of BCG TI as a strategy to efficiently control Babesia infection.