bluuebunny/crossref_metadata_2025 huggingface dataset:
Deconstruction in Collections of Ukrainian Designers: Specific Characteristics and Ways of Interpretation
Authors:   Kharkiv State Academy of Design and Arts, Shevchuk Khrystyna
Published: May 2023

This paper is a research of deconstructivism features in Ukrainian design within 2010 — 2021, the main artistic-compositional and expressive means of deconstruction in Ukrainian fashion are traced, the peculiarities of their interpretation and characteristic combinations of features are analyzed. The main manifestations of deconstructivism in modern Ukrainian design are systematized and generalized on the basis of visual research, using the analytical method and the method of comparative historical analysis. To highlight the specific features of fashion deconstruction in Ukraine in the general context of the phenomenon, a structural-functional method is used. An analysis of clothing collections of the last decade has proved that Ukrainian design often shows such signs of deconstructivism as operating with artistic techniques and symbols of other arts and eras, emphasizing individual elements and their combinations, deliberate exaggeration, using the principle of asymmetry in clothing constructing (asymmetry of sewing or decor), dynamic, contrast and chaotic clothing designs, departure from the classic rules of construction of clothing — moving elements, replacing them, seeming illogicality. It is argued and shown on specific examples that Ukrainian designers follow limit color and textures — natural colors for shaping, less often use accent color mix. Typical means of decoration in terms of deconstruction, such as technological imperfections, cuts, holes, unobstructed edges of products, specifics of the use of non-textile materials are illustrated. The peculiarities of deconstruction manifestations in Ukrainian clothing design and characteristic combinations of features in the work of such clothing brands as Elena Burenina, Dzhus, Ostel, Balossa, Juliya Kros, Vozianiv and others are analyzed. Scientific novelty lies in systematic research of the main features of deconstruction of Ukrainian design at the beginning of the 21st century, outlines the boundaries of the phenomenon to define in modern Ukrainian fashion. The results of the study can be used by designers to create fashion products, as well as by researchers for an in-depth study of the phenomenon of reconstruction.
Effect of Ni-based Fillers on Corrosion Resistance of Heat Affected Zone of Super Austenitic Stainless Steel (UNS S31254) Welded by GTAW
Authors: Park Jin Sung , Cho Dong Min , Hong Seung Gab , Yun Tae Ho , Kim Sung Jin
Published: May 2023

The effect of Ni-based fillers on corrosion resistance of welded super austenitic stainless steel (SASS) was examined. The use of Ni-based fillers for gas tungsten arc welded SASS leads to the formation of fine Cr-Mo enriched phase in its weld unmixed zone, which makes it more susceptible to the localized corrosion. The metallographic observations showed that the pits were initiated around the fine Cr-Mo enriched phase precipitated in unmixed zone, and they were propagated along the base metal. The degree of corrosion damage among the welded samples using the three types of Ni-based fillers (Inconel 625, Inconel 622, Hastelloy 276) increased with the Mo contents in the fillers applied. The higher the Mo contents in the fillers, the higher the size/number ratio of Cr-Mo enriched phase with higher concentration of Mo, precipitated in unmixed zone. Based on the results, it is proposed that the Mo contents in Ni-based high alloyed fillers can have a great effect on the precipitation behavior of Cr-Mo enriched phase in unmixed zone, causing deterioration of corrosion resistance of SASS welds. Therefore, the Mo content in the filler should be optimized with the content similar to that of the base metal so as not to susceptible to localized corrosion.
Detection of Undiagnosed Elevated Cardiovascular Risk Biomarkers among HIV-Positive Patients on Antiretroviral Therapy (ART) in Kigali-City, Rwanda
Authors: Bushaku Marcus , Nyamwange Caleb , Kwena Arthur , Twagirumukiza Marc , Utumatwishima Jean Nepo , Mugeni Regine , Davila-Roman Victor , Nishimwe Aurore , Schectman Kenneth , Cade W Todd , Reeds Dominic , Mutimura Eugene
Published: May 2023

Background: Similar to other African countries, life expectancy of people living with HIV infection has improved due to access to antiretroviral therapy (ART) in Rwanda. However, both HIV infection and use of ART are associated with cardiovascular disease (CVD) risks, due to adverse changes in some biomarkers, causing dyslipidemia and other metabolic imbalances. Biomarkers for CVD risk in HIV-infected individuals taking ART, has not been well characterized in Rwanda. We evaluated the association between the use and duration of ART and biomarkers of CVD risk among HIV infected adults in Rwanda.

Methods: A total of 150 participants (18-45 years) from HIV clinics in public Health Center in Kigali included n=30 HIV-uninfected (HIV-) and n=120 HIV-infected (HIV+) adults. Among the HIV+ adults, n=40 participants were ART-naïve. Cross-sectional data were collected on healthrelated behaviors and biochemical markers of CVD risk. We compared CVD-related biomarkers between HIV-, HIV+ ART-naïve, and HIV+ on ART groups.

Results: The majority of participants were women (60%) and HIV- were younger (35 ± 6 vs. 31 ± 6 years). Total cholesterol and triglycerides concentrations were associated with ART usage. Serum triglycerides concentrations were lower in HIV+ ART-naïve compared to HIV+ on ART (76.6 ± 38.9 mg/dl vs. 85.0 ± 38.3 mg/dl; p< 0.01). While total cholesterol concentrations were higher in HIV+ on ART than HIV+ ART-naïve (136.0 ± 45.1 mg/dl vs. 130.0 ± 36.5 mg/dl; p<0.04), HDL-C was higher in those taking ART (68.7 ± 30.0 mg/dl vs. 55.0 ± 25.7 mg/dl; p=0.02) among HIV+ on ART for 0-6 months and 7-12 months respectively.

Conclusion: Elevated levels of cardiovascular risk biomarker profiles (serum total cholesterol and triglycerides) were associated with use of ART in young adults with HIV in the present study. Although these values were within the upper limits of normal, our findings suggest early alterations in biomarkers of cardiovascular risk. These findings underscore the need for early evaluation of lipid profiles as biomarkers of CVD risk, to effectively monitor how ART may contribute to cardiovascular disease and deter treatment programs in Rwanda and other African countries.
IL-1 in Abdominal Aortic Aneurysms
Authors: Millar Jessica , Nasser Elias , Ailawadi Gorav , Salmon Morgan
Published: May 2023

Abdominal Aortic Aneurysms (AAA) remain a clinically devastating disease with no effective medical treatment therapy. AAAs are characterized by immune cell infiltration, smooth muscle cell apoptosis, and extracellular matrix degradation. Interleukin-1 (IL-1) has been shown to play role in AAA associated inflammation through immune cell recruitment and activation, endothelial dysfunction, production of reactive oxygen species (ROS), and regulation of transcription factors of additional inflammatory mediators. In this review, we will discuss the principles of IL-1 signaling, its role in AAA specific inflammation, and regulators of IL-1 signaling. Additionally, we will discuss the influence of genetic and pharmacological inhibitors of IL-1 on experimental AAAs. Evidence suggests that IL-1 may prove to be a potential therapeutic target in the management of AAA disease.
Genç Kadınlarda Siber Zorbalık ve Siber Mağduriyet Düzeyinin İncelenmesi
Authors: BALMUMCU Ayça , YÜCEYILMAZ Hilal
Published: May 2023

Bu çalışmada üniversitede öğrenim gören genç kadınlarda siber zorbalık ve siber mağduriyet düzeylerinin incelenmesi amaçlanmıştır. Tanımlayıcı tipte olan araştırmanın çalışma grubunu bir üniversitede 15 Ekim-1 Aralık 2022 tarihleri arasında, öğrenim gören 450 kız öğrenci oluşturmuştur. Araştırma verilerinin toplanmasında “Tanımlayıcı anket formu” ve “Üniversite Öğrencileri için Yenilenmiş Siber Zorbalık Ölçeği” kullanılmıştır. Araştırma sonucunda öğrencilerin siber zorbalık ölçek toplam puan ortalaması 12.65 ± 3.61, siber mağduriyet ölçek toplam puan ortalaması ise 15.4 ± 5.38 olarak bulunmuştur. Siber zorbalık ve siber mağduriyet ölçekleri arasında anlamlı bir ilişki saptanmıştır (p
Assessment of indoor air quality and comfort by comparing an energy simulation and actual data in Native American shelters
Authors: Fowler Paul , Del Ama Gonzalo Fernando , Newell Sarah , Poolman James , Montero Burgos Maria J. , González Lezcano Roberto Alonso
Published: May 2023

Introduction: This research will determine if a native American shelter (wigwam) can create comfort and if while doing so can provide healthy indoor air quality (IAQ) levels as defined by current standards. Concurrent to this research a technique to digitally model the outcomes of comfort created within the shelter was developed.

Methods: A fullsize example of a wigwam was built and data from inside and outside the wigwam monitored for comparison. Data collected both inside and outside was temperature and relative humidity of the air, collected inside the wigwam were CO2, VOC, and PM2.5 levels. The wigwam allowed us to compare the accuracy of a digital model created in Design Builder. The Design Builder model was made to the specific size, materials, and location of the actual wigwam. This allowed an accurate comparison of temperature and relative humidity levels. Design-Builder accurately recreated the attributes of the full-size wigwam.

Results and Discussion: It was found that comfort can be achieved to modern standards in this native shelter; as temperature, relative humidity, and rainfall exposure can all be controlled to acceptable levels. Indoor air quality is always at an acceptable level when a fire isn’t active. When an open fire is introduced, the particulates and VOC released into the interior of the wigwam are at dangerous levels. A woodstove with flue pipe allowed for comfort to be maintained at healthier air quality levels but did not reach acceptable levels for particulate matter.
Epithelial cells sense local stiffness via Piezo1 mediated cytoskeletal reorganization
Authors: Jetta Deekshitha , Shireen Tasnim , Hua Susan Z.
Published: May 2023

Local substrate stiffness is one of the major mechanical inputs for tissue organization during its development and remodeling. It is widely recognized that adherent cells use transmembrane proteins (integrins) at focal adhesions to translate ECM mechanical cues into intracellular bioprocess. Here we show that epithelial cells respond to substrate stiffening primarily via actin cytoskeleton organization, that requires activation of mechanosensitive Piezo1 channels. Piezo1 Knockdown cells eliminated the actin stress fibers that formed on stiff substrates, while it had minimal effect on cell morphology and spreading area. Inhibition of Piezo1 channels with GsMTx4 also significantly reduced stiffness-induced F-actin reorganization, suggesting Piezo1 mediated cation current plays a role. Activation of Piezo1 channels with specific agonist (Yoda1) resulted in thickening of F-actin fibers and enlargement of FAs on stiffer substrates, whereas it did not affect the formation of nascent FAs that facilitate spreading on the soft substrates. These results demonstrate that Piezo1 functions as a force sensor that couples with actin cytoskeleton to distinguish the substrate stiffness and facilitate epithelial adaptive remodeling.
Isolation and in vitro characterization of novel S. epidermidis phages for therapeutic applications
Authors: Štrancar Vida , Marušić Monika , Tušar Jasmina , Praček Neža , Kolenc Marko , Šuster Katja , Horvat Simon , Janež Nika , Peterka Matjaž
Published: May 2023

S. epidermidis is an important opportunistic pathogen causing chronic prosthetic joint infections associated with biofilm growth. Increased tolerance to antibiotic therapy often requires prolonged treatment or revision surgery. Phage therapy is currently used as compassionate use therapy and continues to be evaluated for its viability as adjunctive therapy to antibiotic treatment or as an alternative treatment for infections caused by S. epidermidis to prevent relapses. In the present study, we report the isolation and in vitro characterization of three novel lytic S. epidermidis phages. Their genome content analysis indicated the absence of antibiotic resistance genes and virulence factors. Detailed investigation of the phage preparation indicated the absence of any prophage-related contamination and demonstrated the importance of selecting appropriate hosts for phage development from the outset. The isolated phages infect a high proportion of clinically relevant S. epidermidis strains and several other coagulase-negative species growing both in planktonic culture and as a biofilm. Clinical strains differing in their biofilm phenotype and antibiotic resistance profile were selected to further identify possible mechanisms behind increased tolerance to isolated phages.
Functional adaptive resources for large herbivores in African savannas: an ecological-gradient based framework
Authors: Fynn Richard W. S. , Provenza Frederick D.
Published: May 2023

The viability of large herbivore populations in the face of climate change, environmental variability, disease and predation will be determined by their freedom to assess and respond to these factors through access to a range of functional seasonal resources and habitats. Their responses will be contingent upon various organismal traits, such as body size, mouth and digestive anatomy, which also facilitate coexistence among sympatric species. In this paper we develop a functional adaptive forage resource framework based on plant biomass, quality and phenology responses on ecological productivity gradients. We show how large herbivores coexist and respond to environmental variability, disease and predation by their foraging responses in relation to functional adaptive resources, as mediated by their anatomical traits. Below a critical body size, where predation limits population size, large herbivores adopt a variety of predation avoidance strategies, which are linked to their anatomical traits and foraging strategies. Mouth anatomy, and its interaction with body size, appears to be the major anatomical trait determining large herbivore selection for grass height. Body size is the major trait determining vulnerability to predators. Ecological productivity gradients underlain by variation in soil moisture availability over the annual cycle support high quality forage in the least productive (driest) regions, which promote growth and reproduction (a fecundity resource). Reserves of adequate quality forage in moderate productivity regions and buffers of low-quality forage in wetter and more productive regions of the gradient prevent loss of body stores over the dry season and starvation during droughts. Fire and grazing contribute towards providing high quality forage by removing old, low-quality material and preventing forage maturation. Consuming a high diversity of plant species distributed across ecological gradients promotes phytochemical diversity in the diet, which functions as medicinal resources to promote health while combating disease and parasites. Large herbivores are becoming increasingly restricted by ecosystem fragmentation in their access to the full range of these functional resource classes. The negative consequences for large herbivore populations of reduced access to these resource classes is compounded by climate change, where conditions are hotter and drought frequency and intensity is expected to be higher.
Carotid atherosclerotic plaque segmentation in multi-weighted MRI using a two-stage neural network: advantages of training with high-resolution imaging and histology
Authors: Li Ran , Zheng Jie , Zayed Mohamed A. , Saffitz Jeffrey E. , Woodard Pamela K. , Jha Abhinav K.
Published: May 2023

Introduction

A reliable and automated method to segment and classify carotid artery atherosclerotic plaque components is needed to efficiently analyze multi-weighted magnetic resonance (MR) images to allow their integration into patient risk assessment for ischemic stroke. Certain plaque components such as lipid-rich necrotic core (LRNC) with hemorrhage suggest a greater likelihood of plaque rupture and stroke event. Assessment for presence and extent of LRNC could assist in directing treatment with impact upon patient outcomes.

Methods

To address the need to accurately determine the presence and extent of plaque components on carotid plaque MRI, we proposed a two-staged deep-learning-based approach that consists of a convolutional neural network (CNN), followed by a Bayesian neural network (BNN). The rationale for the two-stage network approach is to account for the class imbalance of vessel wall and background by providing an attention mask to the BNN. A unique feature of the network training was to use ground truth defined by both high-resolution ex vivo MRI data and histopathology. More specifically, standard resolution 1.5 T in vivo MR image sets with corresponding high resolution 3.0 T ex vivo MR image sets and histopathology image sets were used to define ground-truth segmentations. Of these, data from 7 patients was used for training and from the remaining two was used for testing the proposed method. Next, to evaluate the generalizability of the method, we tested the method with an additional standard resolution 3.0 T in vivo data set of 23 patients obtained from a different scanner.

Results

Our results show that the proposed method yielded accurate segmentation of carotid atherosclerotic plaque and outperforms not only manual segmentation by trained readers, who did not have access to the ex vivo or histopathology data, but also three state-of-the-art deep-learning-based segmentation methods. Further, the proposed approach outperformed a strategy where the ground truth was generated without access to the high resolution ex vivo MRI and histopathology. The accurate performance of this method was also observed in the additional 23-patient dataset from a different scanner.

Conclusion

In conclusion, the proposed method provides a mechanism to perform accurate segmentation of the carotid atherosclerotic plaque in multi-weighted MRI. Further, our study shows the advantages of using high-resolution imaging and histology to define ground truth for training deep-learning-based segmentation methods.
The effects of cardiac structure, valvular regurgitation, and left ventricular diastolic dysfunction on the diagnostic accuracy of Murray law–based quantitative flow ratio
Authors: Yang Junqing , Huang Yuming , Li Xiaoshan , Jia Qianjun , Deng Huiliang , Xie Nianjin , Huang Meiping , Fei Hongwen
Published: May 2023

Objective

The study aimed to investigate the diagnostic accuracy of Murray law–based quantitative flow ratio (μQFR) from a single angiographic view in patients with abnormal cardiac structure, left ventricular diastolic dysfunction, and valvular regurgitation.

Background

μQFR is a novel fluid dynamics method for deriving fractional flow reserve (FFR). In addition, current studies of μQFR mainly analyzed patients with normal cardiac structure and function. The accuracy of μQFR when patients had abnormal cardiac structure, left ventricular diastolic dysfunction, and valvular regurgitation has not been clear.

Methods

This study retrospectively analyzed 261 patients with 286 vessels that underwent both FFR and μQFR prior to intervention. The cardiac structure and function were measured using echocardiography. Pressure wire–derived FFR ≤0.80 was defined as hemodynamically significant coronary stenosis.

Results

μQFR had a moderate correlation with FFR (r = 0.73, p < 0.001), and the Bland–Altman plot presented no difference between the μQFR and FFR (0.006 ± 0.075, p = 0.192). With FFR as the standard, the diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of μQFR were 94.06% (90.65–96.50), 82.56% (72.87–89.90), 99.00% (96.44–99.88), 97.26 (89.91–99.30), and 92.96% (89.29–95.44), respectively. The concordance of μQFR/FFR was not associated with abnormal cardiac structure, valvular regurgitation (aortic valve, mitral valve, and tricuspid valve), and left ventricular diastolic function. Coronary hemodynamics showed no difference between normality and abnormality of cardiac structure and left ventricular diastolic function. Coronary hemodynamics demonstrated no difference among valvular regurgitation (none, mild, moderate, or severe).

Conclusion

μQFR showed an excellent agreement with FFR. The effect of abnormal cardiac structure, valvular regurgitation, and left ventricular diastolic function did not correlate with the diagnostic accuracy of μQFR. Coronary hemodynamics showed no difference in patients with abnormal cardiac structure, valvular regurgitation, and left ventricular diastolic function.
Associations of adenovirus-reactive immunoglobulins with atrial fibrillation and body mass index
Authors: Gumanova Nadezhda G. , Zlobina Polina D. , Bogdanova Natalya L. , Brutyan Hakob A. , Kalemberg Elena N. , Metelskaya Victoria A. , Davtyan Karapet V. , Drapkina Oksana M.
Published: May 2023

Adenovirus (AdV) has been suggested to be involved in pathogenesis of atrial fibrillation (AF). We aimed to evaluate an association between AdV-specific immunoglobulins G in the serum (AdV-IgG) and AF. The present case-control study comprised two cohorts, including cohort 1 of patients with AF and cohort 2 of asymptomatic subjects. Initially, two groups, MA and MB, were selected from the cohorts 1 and 2, respectively, for serum proteome profiling using an antibody microarray to identify possible relevant protein targets. The data of microarray analysis indicated a possible overall increase in the total adenovirus signals in the group MA vs. group MB, suggesting potential relevance of adenoviral infection to AF. Then, the groups A (with AF) and B (control) were selected from the cohorts 1 and 2, respectively, to assay the presence and levels of AdV-IgG- by ELSA. The prevalence of AdV-IgG-positive status demonstrated a 2-fold increase in the group A (AF) compared with that in the group B (asymptomatic subjects); odds ratio 2.06 (95%CI: 1.11–3.84; P = 0.02). The prevalence of obesity demonstrated an approximately 3-fold increase in AdV-IgG-positive patients of the group A compared with that in AdV-IgG-negative patients of the same group A (odds ratio 2.7; 95% CI: 1.02–7.1; P = 0.04). Thus, AdV-IgG-positive reactivity was independently associated with AF, and AF was independently associated with BMI, indicating that adenoviral infection may be a possible etiological factor for AF.
Crustal structure and the seismogenic environment in Yunnan imaged by double-difference tomography
Authors: Sun Ya , Deng Shilin , Huang Jiwen
Published: May 2023

The large-scale faulting and earthquake activities that developed extensively in the Yunnan area are associated with the collision of India and Eurasia. The fine crustal structure can provide a better understanding of the crustal deformation, seismogenic environment, and rupture processes. We performed a new 3-dimensional (3D) P wave velocity structure and seismic relocation using double-difference tomography based on seismic observations. The tomography images show that large-scale low-velocity anomalies spread around the margin of the south Chuan–Dian Block, Xiaojiang fault (XJF), and the Lijiang–Xiaojinhe fault (LJ-XJHF) in the middle and lower crust. There is an obvious high-speed anomaly in the Emeishan large igneous province (ELIP). We infer that the low-velocity anomaly under the LJ-XJHF zone may be derived from the lower crustal flow extruded from the central Tibetan plateau and obstructed by the ELIP, while the velocity anomalies around the XJF might be caused by shear heating, which is associated with the large-deep strike–slip fault and the transmission of stress in the southeast direction. The inversion results also show that the Yangbi earthquake occurred at the NW–SE boundary of high and low velocity from the upper crust to the lower crust, which coincides well with the location of the Yangbi earthquake sequence and the Weixi–Qiaohou fault. Meanwhile, the earthquake relocations show that the aftershocks are mainly distributed at low velocities. All the aforementioned research results indicate that the Yangbi earthquake might be attributed to the intrusion of the soft material flow along the Weixi–Qiaohou fault in the NW–SE direction. These low-viscosity crustal materials would cause brittle fractures and result in NW–SE sinistral strike–slip faults.
Experiences of precarious work within higher education institutions: a qualitative evidence synthesis
Authors: Solomon Shihaam , Du Plessis Marieta
Published: May 2023

Introduction

A qualitative evidence synthesis was employed, to identify and synthesize the best evidence on the experiences of precariously employed academics in high education institutions.

Methods

The Joanna Briggs Institute (JBI) principles were followed. The identified studies were screened by titles and abstracts (n = 308)-full-text (n = 19), employing these inclusion criteria: studies reporting on precarious employment experiences in higher education; part-time or fixed-term academic positions; qualitative studies between 2010 to 2021. The selected studies were not limited to a particular geographical location. A quality appraisal was conducted. Data were extracted while findings from the included studies (n = 19) were collated using meta-aggregation with the Joanna Briggs Institute Qualitative Assessment and Review Instrument (JBI-QARI). The primary study findings emanated from research conducted across 14 countries both from the northern and southern hemispheres.

Results

Ninety-four extracted findings were aggregated into 19 categories and then grouped into five synthesized findings: (1) Precarity is created and perpetuated through structural changes in the global economy and wider higher education landscape; (2) Coping strategies precariously employed academics used to endure precarious employment in higher education; (3) Gendered dimensions shaping employment precarity in academia; (4) Impact of precarious employment on academics; (5) Impact of academic precarity on the university.

Discussion

These precariously employed academics felt overwhelmed, vulnerable, exploited, stressed, anxious, and exhausted with their employment conditions. These circumstances include operating in unstable and insecure employment with no guarantees of permanent employment. The need to reassess policies and practices within higher education institutions is necessary and could offer these precariously employed academics the much-needed support and assistance to combat the effects of precarious employment.
The osteocyte and its osteoclastogenic potential
Authors: Marahleh Aseel , Kitaura Hideki , Ohori Fumitoshi , Noguchi Takahiro , Mizoguchi Itaru
Published: May 2023

The skeleton is an organ of dual functionality; on the one hand, it provides protection and structural competence. On the other hand, it participates extensively in coordinating homeostasis globally given that it is a mineral and hormonal reservoir. Bone is the only tissue in the body that goes through strategically consistent bouts of bone resorption to ensure its integrity and organismal survival in a temporally and spatially coordinated process, known as bone remodeling. Bone remodeling is directly enacted by three skeletal cell types, osteoclasts, osteoblasts, and osteocytes; these cells represent the acting force in a basic multicellular unit and ensure bone health maintenance. The osteocyte is an excellent mechanosensory cell and has been positioned as the choreographer of bone remodeling. It is, therefore, not surprising that a holistic grasp of the osteocyte entity in the bone is warranted. This review discusses osteocytogenesis and associated molecular and morphological changes and describes the osteocytic lacunocanalicular network (LCN) and its organization. We highlight new knowledge obtained from transcriptomic analyses of osteocytes and discuss the regulatory role of osteocytes in promoting osteoclastogenesis with an emphasis on the case of osteoclastogenesis in anosteocytic bones. We arrive at the conclusion that osteocytes exhibit several redundant means through which osteoclast formation can be initiated. However, whether osteocytes are true “orchestrators of bone remodeling” cannot be verified from the animal models used to study osteocyte biology in vivo. Results from studying osteocyte biology using current animal models should come with the caveat that these models are not osteocyte-specific, and conclusions from these studies should be interpreted cautiously.
Age-related modifications in CYP-dependent drug metabolism: role of stress
Authors: Konstandi Maria , Johnson Elizabeth O.
Published: May 2023

Accumulating clinical evidence indicates extensive inter-individual variations in the effectiveness and adverse effects of standard treatment protocols, which are largely attributed to the multifactorial regulation of the hepatic CYP-dependent drug metabolism that is connected with either transcriptional or post-translational modifications. Age and stress belong to the most important factors in CYP gene regulation. Alterations in neuroendocrine responses to stress, which are associated with modified hypothalamo-pituitary-adrenal axis function, usually accompany ageing. In this light, ageing followed by a decline of the functional integrity of organs, including liver, a failure in preserving homeostasis under stress, increased morbidity and susceptibility to stress, among others, holds a determinant role in the CYP-catalyzed drug metabolism and thus, in the outcome and toxicity of pharmacotherapy. Modifications in the drug metabolizing capacity of the liver with age have been reported and in particular, a decline in the activity of the main CYP isoforms in male senescent rats, indicating decreased metabolism and higher levels of the drug-substrates in their blood. These factors along with the restricted experience in the use of the most medicines in childhood and elderly, could explain at an extent the inter-individual variability in drug efficacy and toxicity outcomes, and underscore the necessity of designing the treatment protocols, accordingly.
Implementation of a patient-centered remote wound monitoring system for management of diabetic foot ulcers
Authors: Keegan Alana C. , Bose Sanuja , McDermott Katherine M. , Starks White Midori P. , Stonko David P. , Jeddah Danielle , Lev-Ari Eilat , Rutkowski Joanna , Sherman Ronald , Abularrage Christopher J. , Selvin Elizabeth , Hicks Caitlin W.
Published: May 2023

Background

Regular clinical assessment is critical to optimize lower extremity wound healing. However, family and work obligations, socioeconomic, transportation, and time barriers often limit patient follow-up. We assessed the feasibility of a novel, patient-centered, remote wound management system (Healthy.io Minuteful for Wound Digital Management System) for the surveillance of lower extremity wounds.

Methods

We enrolled 25 patients from our outpatient multidisciplinary limb preservation clinic with a diabetic foot ulcer, who had undergone revascularization and podiatric interventions prior to enrollment. Patients and their caregivers were instructed on how to use the digital management system and asked to perform one at-home wound scan per week for a total of 8 weeks using a smartphone application. We collected prospective data on patient engagement, smartphone app useability, and patient satisfaction.

Results

Twenty-five patients (mean age 65.5 ± 13.7 years, 60.0% male, 52.0% Black) were enrolled over 3 months. Mean baseline wound area was 18.0 ± 15.2 cm2, 24.0% of patients were recovering from osteomyelitis, and post-surgical WiFi stage was 1 in 24.0%, 2 in 40.0%, 3 in 28.0%, and 4 in 8.00% of patients. We provided a smartphone to 28.0% of patients who did not have access to one that was compatible with the technology. Wound scans were obtained by patients (40.0%) and caregivers (60.0%). Overall, 179 wound scans were submitted through the app. The mean number of wound scans acquired per patient was 0.72 ± 0.63 per week, for a total mean of 5.80 ± 5.30 scans over the course of 8 weeks. Use of the digital wound management system triggered an early change in wound management for 36.0% of patients. Patient satisfaction was high; 94.0% of patients reported the system was useful.

Conclusion

The Healthy.io Minuteful for Wound Digital Management System is a feasible means of remote wound monitoring for use by patients and/or their caregivers.
V2 vasopressin receptor mutations: future personalized therapy based on individual molecular biology
Authors: Erdélyi László Sándor , Hunyady László , Balla András
Published: May 2023

The diluting and concentrating function of the kidney plays a crucial role in regulating the water homeostasis of the body. This function is regulated by the antidiuretic hormone, arginine vasopressin through the type 2 vasopressin receptor (V2R), allowing the body to adapt to periods of water load or water restriction. Loss-of-function mutations of the V2R cause X-linked nephrogenic diabetes insipidus (XNDI), which is characterized by polyuria, polydipsia, and hyposthenuria. Gain-of-function mutations of the V2R lead to nephrogenic syndrome of inappropriate antidiuresis disease (NSIAD), which results in hyponatremia. Various mechanisms may be responsible for the impaired receptor functions, and this review provides an overview of recent findings about the potential therapeutic interventions in the light of the current experimental data.
SARS-CoV-2 bivalent mRNA vaccine with broad protection against variants of concern
Authors: Ma Qinhai , Li Man , Ma Lin , Zhang Caroline , Zhang Hong , Zhong Huiling , Wen Jian , Wang Yongsheng , Yan Zewei , Xiong Wei , Wu Linping , Guo Jianmin , Yang Wei , Yang Zifeng , Zhang Biliang
Published: May 2023

Introduction

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant has rapidly spread around the globe. With a substantial number of mutations in its Spike protein, the SARS-CoV-2 Omicron variant is prone to immune evasion and led to the reduced efficacy of approved vaccines. Thus, emerging variants have brought new challenges to the prevention of COVID-19 and updated vaccines are urgently needed to provide better protection against the Omicron variant or other highly mutated variants.

Materials and methods

Here, we developed a novel bivalent mRNA vaccine, RBMRNA-405, comprising a 1:1 mix of mRNAs encoding both Delta-derived and Omicron-derived Spike proteins. We evaluated the immunogenicity of RBMRNA-405 in BALB/c mice and compared the antibody response and prophylactic efficacy induced by monovalent Delta or Omicron-specific vaccine with the bivalent RBMRNA-405 vaccine in the SARSCoV-2 variant challenge.

Results

Results showed that the RBMRNA-405 vaccine could generate broader neutralizing antibody responses against both Wuhan-Hu-1 and other SARS-CoV-2 variants, including Delta, Omicron, Alpha, Beta, and Gamma. RBMRNA-405 efficiently blocked infectious viral replication and lung injury in both Omicron- and Delta-challenged K18-ACE2 mice.

Conclusion

Our data suggest that RBMRNA-405 is a promising bivalent SARS-CoV-2 vaccine with broad-spectrum efficacy for further clinical development.
Combination of violet light irradiation and collagenase treatments in a rabbit model of keratoconus
Authors: Kobashi Hidenaga , Yano Takashi , Tsubota Kazuo
Published: May 2023

Purpose

We evaluated the use of collagenase treatment to generate a rabbit model of keratoconus and the impact of violet light (VL) irradiation on the disease model in six Japanese White rabbits.

Methods

After epithelial debridement, the collagenase group was treated with a collagenase type II solution for 30 min; the control group was treated with a solution without collagenase. Three rabbits also underwent VL irradiation (375 nm, irradiance 310 μW/cm2) for 3 h daily for 7 days after topical collagenase application. Slit-lamp microscopy results, steep keratometry (Ks), corneal astigmatism, central corneal thickness, and axial length were examined before and after the procedure. The corneas were obtained on day 7 for biomechanical evaluation.

Results

A significant increase in Ks and corneal astigmatism was observed in the collagenase and VL irradiation groups compared with the control group on day 7. No significant difference was found in the change in corneal thickness between the groups. The elastic modulus at 3, 5, and 10% strain was significantly lower in the collagenase group than in the control group. There was no significant difference in the elastic modulus at any level of strain between the collagenase and VL irradiation groups. The average axial length at day 7 was significantly longer in the collagenase and VL irradiation groups than in the control group. Collagenase treatment induced a model of keratoconus by steepening the keratometric and astigmatic values. There was no significant difference in the observed elastic behavior of normal and ectatic corneas under physiologically relevant stress levels.

Conclusion

VL irradiation did not cause regression of corneal steepening in a collagenase-induced model during short-term observation.
Determining clinical biomarkers to predict long-term SARS-CoV-2 antibody response among COVID-19 patients in Bangladesh
Authors: Ahmed Tasnuva , Hasan S. M. Tafsir , Akter Afroza , Tauheed Imam , Akhtar Marjahan , Rahman Sadia Isfat Ara , Bhuiyan Taufiqur Rahman , Ahmed Tahmeed , Qadri Firdausi , Chowdhury Fahima
Published: May 2023

Background

Information on antibody responses following SARS-CoV-2 infection, including the magnitude and duration of responses, is limited. In this analysis, we aimed to identify clinical biomarkers that can predict long-term antibody responses following natural SARS-CoV-2 infection.

Methodology

In this prospective study, we enrolled 100 COVID-19 patients between November 2020 and February 2021 and followed them for 6 months. The association of clinical laboratory parameters on enrollment, including lactate dehydrogenase (LDH), neutrophil–lymphocyte ratio (NLR), C-reactive protein (CRP), ferritin, procalcitonin (PCT), and D-dimer, with predicting the geometric mean (GM) concentration of SARS-CoV-2 receptor-binding domain (RBD)-specific IgG antibody at 3 and 6 months post-infection was assessed in multivariable linear regression models.

Result

The mean ± SD age of patients in the cohort was 46.8 ± 14 years, and 58.8% were male. Data from 68 patients at 3 months follow-up and 55 patients at 6 months follow-up were analyzed. Over 90% of patients were seropositive against RBD-specific IgG till 6 months post-infection. At 3 months, for any 10% increase in absolute lymphocyte count and NLR, there was a 6.28% (95% CI: 9.68, −2.77) decrease and 4.93% (95% CI: 2.43, 7.50) increase, respectively, in GM of IgG concentration, while any 10% increase for LDH, CRP, ferritin, and procalcitonin was associated with a 10.63, 2.87, 2.54, and 3.11% increase in the GM of IgG concentration, respectively. Any 10% increase in LDH, CRP, and ferritin was similarly associated with an 11.28, 2.48, and 3.0% increase in GM of IgG concentration at 6 months post-infection.

Conclusion

Several clinical biomarkers in the acute phase of SARS-CoV-2 infection are associated with enhanced IgG antibody response detected after 6 months of disease onset. The measurement of SARS-CoV-2 specific antibody responses requires improved techniques and is not feasible in all settings. Baseline clinical biomarkers can be a useful alternative as they can predict antibody response during the convalescence period. Individuals with an increased level of NLR, CRP, LDH, ferritin, and procalcitonin may benefit from the boosting effect of vaccines. Further analyses will determine whether biochemical parameters can predict RBD-specific IgG antibody responses at later time points and the association of neutralizing antibody responses.
Case report: Sulfasalazine-induced hypersensitivity
Authors: Kuchinskaya Ekaterina M. , Chikova Irina A. , Kostik Mikhail M.
Published: May 2023

Drug-induced hypersensitivity syndrome (DiHS)/drug reaction with eosinophilia and systemic symptoms (DRESS) is a systemic inflammatory condition that is characterized by multisystemic involvement (liver, blood, and skin), heterogeneous manifestations (fever, rash, lymphadenopathy, and eosinophilia), and an unpredictable course; cases of DiHS/DRESS caused by sulfasalazine are rare in children compared to adults. We report a case of a 12-year-old girl with juvenile idiopathic arthritis (JIA) and sulfasalazine-related hypersensitivity who developed fever, rash, blood abnormalities, and hepatitis complicated with hypocoagulation. The treatment with intravenous and then oral glucocorticosteroids was effective. We also reviewed 15 cases (67% male patients) of childhood-onset sulfasalazine-related DiHS/DRESS from the MEDLINE/PubMed and Scopus online databases. All reviewed cases had a fever, lymphadenopathy, and liver involvement. Eosinophilia was reported in 60% of patients. All patients were treated with systemic corticosteroids, and one patient required emergency liver transplantation. Two patients (13%) died. A total of 40.0% of patients satisfied RegiSCAR definite criteria, 53.3% were probable, and 80.0% satisfied Bocquet's criteria. Only 13.3% satisfied typical and 20.0% atypical DIHS criteria from the Japanese group. Pediatric rheumatologists should be aware of DiHS/DRESS due to its similarities to other systemic inflammatory syndromes (especially systemic JIA, macrophage activation syndrome, and secondary hemophagocytic lymphohistiocytosis). Further studies of DiHS/DRESS syndrome in children are needed to improve its recognition and differential diagnostic and therapeutic options.
Effects of HBsAg carriers on pregnancy complications in pregnant women: a retrospective cohort study
Authors: Weng Mengqing , Wang Jie , Yin Jingfeng , Ren Wenning , Wei Caiping , Yang Wenshan , He Huimin
Published: May 2023

Objective

Hepatitis B virus (HBV) infection is a major health threat worldwide, especially in developing countries. We aimed to investigate the impact of hepatitis B carrier on pregnancy complications in pregnant women, in China.

Methods

This retrospective cohort study was conducted by using data from the EHR system of Longhua District People’s Hospital in Shenzhen, China, from January 2018 to June 2022. Binary logistic regression was used to evaluate the relationship between HBsAg carrier status and pregnancy complications and pregnancy outcomes.

Results

The study included 2095 HBsAg carriers (exposed group) and 23,019 normal pregnant women (unexposed group). Pregnant women in the exposed group were older than the pregnant women in the unexposed group (29 (27,32) vs. 29 (26,32), p < 0.001). In addition, the incidence of some adverse pregnancy complications in the exposure group was lower than that in the unexposed group, including hypothyroidism of pregnancy (adjusted odds ratio [aOR], 0.779; 95% confidence interval [CI], 0.617–0.984; p = 0.036), hyperthyroidism of pregnancy (aOR, 0.388; 95% CI, 0.159–0.984; p = 0.038), pregnancy induced hypertension (aOR, 0.699; 95% CI, 0.551–0.887; p = 0.003), antepartum hemorrhage (aOR, 0.294; 95% CI, 0.093–0.929; p = 0.037). However, compared with the unexposed group, the exposed group had a higher risk of lower birth weight (aOR, 1.12; 95% CI, 1.02–1.23; p = 0.018) and intrahepatic cholestasis of pregnancy (aOR, 2.888, 95% CI, 2.207–3.780; p < 0.001).

Conclusion

The prevalence rate of HBsAg carriers in pregnant women in Longhua District of Shenzhen was 8.34%. Compared with normal pregnant women, HBsAg carriers have a higher risk of ICP, a lower risk of gestational hypothyroidism and PIH, and a lower birth weight of their infants.
Hemoporfin-mediated photodynamic therapy with general anesthesia showed superior efficacy in the treatment of port-wine stains: a retrospective evaluation
Authors: Hu Yan-Yan , Chen Kai , Wang Lin-Lin , Wang Jia-Fang , Chen Xi , Cao Li-Juan , Jiang Qian , Wang Zhen-Xing , Qian Shan-Shan , Chen Zhi-Jun , Chen Liu-Qing , Li Dong-Sheng
Published: May 2023

Background

Hemoporfin-mediated photodynamic therapy (PDT) is an effective treatment for port-wine stains (PWS), and pain is the main adverse effect of this therapy. General anesthesia is commonly used for pain management during PDT, but the effect of general anesthetics on the subsequent treatment efficacy of PDT in PWS has not been reported.

Objectives

To assess the use of general anesthesia combined with PDT compared with PDT alone in 207 PWS patients, and to provide further safety and efficacy data on this combined therapy.

Methods

Propensity score matching (PSM) was used at a 2:1 ratio to create a general anesthetic group (n = 138) and a highly comparable nonanesthetic group (n = 69). The clinical outcomes were evaluated, and the treatment reactions and adverse effects were recorded after one treatment with PDT.

Results

After matching, there was no significant difference in the demographic data of the patients in the two groups (p > 0.05), while the treatment efficacy was significantly higher in the general anesthetic group than in the nonanesthetic group (76.81 vs. 56.52%, p < 0.05). Moreover, logistic regression analysis confirmed that patients receiving general anesthesia showed an association with a good response to PDT (OR = 3.06; 95% CI, 1.57–6.00; p = 0.0011). Purpura lasted longer in the general anesthetic group, but the other treatment reactions and adverse effects were similar in the two groups (p > 0.05). No serious systemic adverse reactions were observed.

Conclusion

We recommend this combined therapy, which is associated with painless, as a high efficacy treatment option for PWS patients, especially for patients with a poor response to multiple PDT alone treatments.
Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
Authors: Cao Tingshuai , Wang Shangren , Pan Yang , Guo Feng , Wu Bin , Zhang Yingchun , Wang Yujie , Tian Jiaqing , Xing Qingfei , Liu Xiaoqiang
Published: May 2023

Introduction

Semen quality is decreasing worldwide, leading to increased male infertility. This study analyzed the microbiota of the gut, semen, and urine in individuals with semen abnormalities to identify potential probiotics and pathogenic bacteria that affect semen parameters and help develop new methods for the diagnosis and treatment of patients with semen abnormalities.

Methods

We recruited 12 individuals with normal semen parameters (control group), 12 with asthenospermia but no semen hyperviscosity (Group_1), 6 with oligospermia (Group_2), 9 with severe oligospermia or azoospermia (Group_3), and 14 with semen hyperviscosity only (Group_4). The semen, gut, and urine microbiota were examined by analyzing the 16S ribosomal RNA gene sequence using next-generation sequencing.

Results

The gut microbes were clustered into the highest number of operational taxonomic units, followed by urine and semen. Furthermore, the α-diversity of gut microbes was highest and significantly different from that of urine and semen microbiota. The microbiota of the gut, urine, and semen were all significantly different from each other in terms of β-diversity. The gut abundance of Collinsella was significantly reduced in groups 1, 3, and 4. Furthermore, the gut abundance of Bifidobacterium and Blautia was significantly decreased in Group_1, while that of Bacteroides was significantly increased in Group_3. The abundance of Staphylococcus was significantly increased in the semen of groups 1 and 4. Finally, Lactobacillus abundance was significantly reduced in the urine of groups 2 and 4.

Discussion

This study comprehensively describes the differences in intestinal and genitourinary tract microbiota between healthy individuals and those with abnormal semen parameters. Furthermore, our study identified Collinsella, Bifidobacterium, Blautia, and Lactobacillus as potential probiotics. Finally, the study identified Bacteroides in the gut and Staphylococcus in semen as potential pathogenic bacteria. Our study lays the foundation of a new approach to the diagnosis and treatment of male infertility.