bluuebunny/crossref_metadata_2025 huggingface dataset:
The Alteration of Chloride Homeostasis/GABAergic Signaling in Brain Disorders: Could Oxidative Stress Play a Role?
Authors: Abruzzo Provvidenza M. , Panisi Cristina , Marini Marina
Published: August 2021

In neuronal precursors and immature neurons, the depolarizing (excitatory) effect of γ-Aminobutyric acid (GABA) signaling is associated with elevated [Cl−]i; as brain cells mature, a developmental switch occurs, leading to the decrease of [Cl−]i and to the hyperpolarizing (inhibitory) effect of GABAergic signaling. [Cl−]i is controlled by two chloride co-transporters: NKCC1, which causes Cl− to accumulate into the cells, and KCC2, which extrudes it. The ontogenetic upregulation of the latter determines the above-outlined switch; however, many other factors contribute to the correct [Cl−]i in mature neurons. The dysregulation of chloride homeostasis is involved in seizure generation and has been associated with schizophrenia, Down’s Syndrome, Autism Spectrum Disorder, and other neurodevelopmental disorders. Recently, much effort has been put into developing new drugs intended to inhibit NKCC1 activity, while no attention has been paid to the origin of [Cl−]i dysregulation. Our study examines the pathophysiology of Cl− homeostasis and focuses on the impact of oxidative stress (OS) and inflammation on the activity of Cl− co-transporters, highlighting the relevance of OS in numerous brain abnormalities and diseases. This hypothesis supports the importance of primary prevention during pregnancy. It also integrates the therapeutic framework addressed to restore normal GABAergic signaling by counteracting the alteration in chloride homeostasis in central nervous system (CNS) cells, aiming at limiting the use of drugs that potentially pose a health risk.
Long-Term Treatment of Cuban Policosanol Attenuates Abnormal Oxidative Stress and Inflammatory Response via Amyloid Plaques Reduction in 5xFAD Mice
Authors: Kim Jin-Ho , Lim Dong-Kyun , Suh Yoo-Hun , Chang Keun-A
Published: August 2021

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder resulting in cognitive decline or dementia, the number of patients with AD is continuously increasing. Although a lot of great progress has been made in research and development of AD therapeutics, there is no fundamental cure for this disease yet. This study demonstrated the memory-improving effects of Cuban policosanol (PCO) in 5xFAD mice, which is an animal model of AD. Following 4-months of treatment with PCO in 5xFAD mice, we found that the number of amyloid plaques decreased in the brain compared to the vehicle-treated 5xFAD mice. Long-term PCO treatment in 5xFAD mice resulted in the reduction of gliosis and abnormal inflammatory cytokines level (interleukin [IL]-1β, IL-6, and tumor necrosis factor [TNF]-α) in the cortex and hippocampus. Levels of lipid peroxide (4-hydroxynonenal [4-HNE]) and superoxide dismutase (SOD1 and SOD2) levels were also recoverd in the brains of PCO-treated 5xFAD mice. Notably, PCO administration reduced memory deficits in the passive avoidance test, as well as synaptic loss (PSD-95, synaptophysin) in 5xFAD mice. Collectively, we identified the potential effects of PCO as a useful supplement to delay or prevent AD progression by inhibiting the formation of Aβ plaques in the brain.
Effects of pH and Temperature on Water under Pressurized Conditions in the Extraction of Nutraceuticals from Chaga (Inonotus obliquus) Mushroom
Authors: Abu-Reidah Ibrahim M. , Critch Amber L. , Manful Charles F. , Rajakaruna Amanda , Vidal Natalia P. , Pham Thu H. , Cheema Mumtaz , Thomas Raymond
Published: August 2021

Currently, there is increased interest in finding appropriate food-grade green extraction systems capable of extracting these bioactive compounds from dietary mushrooms for applications in various food, pharmacological, or nutraceutical formulations. Herein, we evaluated a modified Swiss water process (SWP) method using alkaline and acidic pH at low and high temperature under pressurized conditions as a suitable green food grade solvent to obtained extracts enriched with myco-nutrients (dietary phenolics, total antioxidants (TAA), vitamins, and minerals) from Chaga. Ultra-high performance liquid chromatography coupled to high resolution accurate mass tandem mass spectrometry (UHPLC-HRAMS-MS/MS) was used to assess the phenolic compounds and vitamin levels in the extracts, while inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the mineral contents. Over 20 phenolic compounds were quantitatively evaluated in the extracts and the highest total phenolic content (TPC) and total antioxidant activity (TAA) was observed at pH 11.5 at 100 °C. The most abundant phenolic compounds present in Chaga extracts included phenolic acids such as protocatechuic acid 4-glucoside (0.7–1.08 µg/mL), syringic acid (0.62–1.18 µg/mL), and myricetin (0.68–1.3 µg/mL). Vitamins are being reported for the first time in Chaga. Not only, a strong correlation was found for TPC with TAA (r-0.8, <0.0001), but also, with individual phenolics (i.e., Salicylic acid), lipophilic antioxidant activity (LAA), and total antioxidant minerals (TAM). pH 2.5 at 100 °C treatment shows superior effects in extracting the B vitamins whereas pH 2.5 at 60 and 100 °C treatments were outstanding for extraction of total fat-soluble vitamins. Vitamin E content was the highest for the fat-soluble vitamins in the Chaga extract under acidic pH (2.5) and high temp. (100 °C) and ranges between 50 to 175 µg/100 g Chaga. Antioxidant minerals ranged from 85.94 µg/g (pH7 at 100 °C) to 113.86 µg/g DW (pH2.5 at 100 °C). High temperature 100 °C and a pH of 2.5 or 9.5. The treatment of pH 11.5 at 100 °C was the most useful for recovering phenolics and antioxidants from Chaga including several phenolic compounds reported for the first time in Chaga. SWP is being proposed herein for the first time as a novel, green food-grade solvent system for the extraction of myco-nutrients from Chaga and have potential applications as a suitable approach to extract nutrients from other matrices. Chaga extracts enriched with bioactive myconutrients and antioxidants may be suitable for further use or applications in the food and nutraceutical industries.
5-Hydroxymaltol Derived from Beetroot Juice through Lactobacillus Fermentation Suppresses Inflammatory Effect and Oxidant Stress via Regulating NF-kB, MAPKs Pathway and NRF2/HO-1 Expression
Authors: Kim Su-Lim , Choi Hack Sun , Ko Yu-Chan , Yun Bong-Sik , Lee Dong-Sun
Published: August 2021

Inflammation is the first response of the immune system against bacterial pathogens. This study isolated and examined an antioxidant derived from Lactobacillus fermentation products using cultured media with 1% beet powder. The antioxidant activity of the beet culture media was significantly high. Antioxidant activity-guided purification and repeated sample isolation yielded an isolated compound, which was identified as 5-hydoxymaltol using nuclear magnetic resonance spectrometry. We examined the mechanism of its protective effect on lipopolysaccharide (LPS)-induced inflammation of macrophages. 5-Hydroxymaltol suppressed nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. It also suppressed tumor necrosis factor α (TNF-α), interleukin (IL)-1β, and inducible nitric oxide synthase (iNOS) in the messenger RNA and protein levels in LPS-treated RAW 264.7 cells. Moreover, it suppressed LPS-induced nuclear translocation of NF-κB (p65) and mitogen-activated protein kinase activation. Furthermore, 5-hydroxymaltol reduced LPS-induced reactive oxygen species (ROS) production as well as increased nuclear factor erythroid 2–related factor 2 and heme oxygenase 1 expression. Overall, this study found that 5-hydroxymaltol has anti-inflammatory activities in LPS-stimulated RAW 264.7 macrophage cells based on its inhibition of pro-inflammatory cytokine production depending on the nuclear factor κB signaling pathway, inhibition of LPS-induced reactive oxygen species production, inhibition of LPS-induced mitogen-activated protein kinase induction, and induction of the nuclear factor erythroid 2–related factor 2/heme oxygenase 1 signaling pathway. Our data showed that 5-hydroxymaltol may be an effective compound for treating inflammation-mediated diseases.
Role of Coenzyme Q10 in Health and Disease: An Update on the Last 10 Years (2010–2020)
Authors: Cirilli Ilenia , Damiani Elisabetta , Dludla Phiwayinkosi Vusi , Hargreaves Iain , Marcheggiani Fabio , Millichap Lauren Elizabeth , Orlando Patrick , Silvestri Sonia , Tiano Luca
Published: August 2021

The present review focuses on preclinical and clinical studies conducted in the last decade that contribute to increasing knowledge on Coenzyme Q10’s role in health and disease. Classical antioxidant and bioenergetic functions of the coenzyme have been taken into consideration, as well as novel mechanisms of action involving the redox-regulated activation of molecular pathways associated with anti-inflammatory activities. Cardiovascular research and fertility remain major fields of application of Coenzyme Q10, although novel applications, in particular in relation to topical application, are gaining considerable interest. In this respect, bioavailability represents a major challenge and the innovation in formulation aspects is gaining critical importance.
Experimental Study of Military Crawl as a Special Type of Human Quadripedal Automatic Locomotion
Authors: Skvortsov Dmitry , Anisimov Victor , Aizenshtein Alina
Published: August 2021

The biomechanics of military crawl locomotion is poorly covered in scientific literature so far. Crawl locomotion may be used as a testing procedure which allows for the detection of not only obvious, but also hidden locomotor dysfunctions. The aim of the study was to investigate the biomechanics of crawling among healthy adult participants. Eight healthy adults aged 15–31 (four women and four men) were examined by means of a 3D kinematic analysis with Optitrack optical motion-capture system which consists of 12 Flex 13 cameras. The movements of the shoulder, elbow, knee, and hip joints were recorded. A person was asked to crawl 4 m on his/her belly. The obtained results including space-time data let us characterize military crawling in terms of pelvic and lower limb motions as a movement similar to walking but at a more primitive level. Progressive and propulsive motions are characterized as normal; additional right–left side motions—with high degree of reciprocity. It was found that variability of the left-side motions is significantly lower than that of the right side (Z = 4.49, p < 0.0001). The given normative data may be used as a standard to estimate the test results for patients with various pathologies of motor control (ataxia, abasia, etc.).
Cybersecurity against the Loopholes in Industrial Control Systems Using Interval-Valued Complex Intuitionistic Fuzzy Relations
Authors: Nasir Abdul , Jan Naeem , Gumaei Abdu , Khan Sami Ullah , Albogamy Fahad R.
Published: August 2021

Technology is rapidly advancing and every aspect of life is being digitalized. Since technology has made life much better and easier, so organizations, such as businesses, industries, companies and educational institutes, etc., are using it. Despite the many benefits of technology, several risks and serious threats, called cyberattacks, are associated with it. The method of neutralizing these cyberattacks is known as cybersecurity. Sometimes, there are uncertainties in recognizing a cyberattack and nullifying its effects using righteous cybersecurity. For that reason, this article introduces interval-valued complex intuitionistic fuzzy relations (IVCIFRs). For the first time in the theory of fuzzy sets, we investigated the relationships among different types of cybersecurity and the sources of cyberattacks. Moreover, the Hasse diagram for the interval-valued complex intuitionistic partial order set and relation is defined. The concepts of the Hasse diagram are used to inspect different cybersecurity techniques and practices. Then, using the properties of Hasse diagrams, the most beneficial technique is identified. Furthermore, the best possible selection of types of cybersecurity is made after putting some restrictions on the selection. Lastly, the advantages of the proposed methods are illuminated through comparison tests.
Design of the High-Speed PMSG with Two Different Shaft Material Considering Overhang Effect and Mechanical Characteristics
Authors: Lee Jeong-In , Bang Tae-Kyoung , Lee Hoon-Ki , Woo Jong-Hyeon , Nah Junghyo , Choi Jang-Young
Published: August 2021

In general, high-speed machines should be designed to satisfy electromagnetic and mechanical characteristics. In this study, the design of high-speed permanent magnet synchronous generator with two different shaft materials considering overhang effect and mechanical characteristics was performed. It was confirmed that the leakage magnetic flux generated by the two shaft materials electromagnetically affects the high-speed generator. Additionally, it is important to accurately predict the natural frequency mode and critical speed to prevent damage and vibration of the rotating body owing to scattering during high-speed rotation. Therefore, the mechanical characteristics of the designed model were analyzed. In this study, we propose a design method that considers both the electromagnetic effects and mechanical characteristics. Subsequently, verification was performed through experiments and comparisons for the validity and reliability of the proposed design method.
Novel Whole Blood MicroRNAs Predicting Chronic Kidney Disease in South Africans with Hypertension and Diabetes Mellitus
Authors: Motshwari Dipuo D. , George Cindy , Matshazi Don M. , Weale Cecil J. , Davids Saarah F. G. , Erasmus Rajiv T. , Kengne Andre P. , Matsha Tandi E.
Published: August 2021

The asymptomatic nature of and lack of effective early-stage diagnostic tools in CKD, predisposes individuals to the risk of end-stage CKD and related complications. Whole blood microRNAs (miRNAs) have the potential for CKD risk screening. We evaluated the expression profile of six novel whole blood miRNAs as well as their ability to predict prevalent CKD in individuals with hypertension and/or diabetes. We included 911 individuals with hypertension and/or diabetes, of which 18.8% had prevalent CKD. The miRNA expression was analyzed using quantitative reverse transcription PCR (RT-PCR). Five of the six miRNAs, namely hsa-miR-novel-chr1_36178, hsa-miR-novel-chr2_55842, hsa-miR-novel-chr7_76196, hsa-miR-novel-chr5_67265, and hsa-miR-novel-chr13_13519, were significantly increased in people with CKD (all p < 0.028). Only the increased expression of hsa-miR-novel-chr2_55842 and hsa-miR-novel-chr7_76196 were independently associated with reduced estimated glomerular filtration rate (eGFR) (both p ≤ 0.038), while all the analyzed miRNAs were positively associated with prevalent CKD (all p ≤ 0.038). All the blood miRNAs were acceptable predictors of CKD (C-statistic > 0.7 for all), with similar predictive capacity (p = 0.202). However, hsa-miR-novel-chr13_13519 added to CKD prediction beyond conventional factors (p = 0.040). Novel whole blood miRNAs showed an acceptable discriminative power to predict prevalent CKD; thereby suggesting the potential use of these miRNAs, particularly hsa-miR-novel-chr13_13519, in clinical practice as a screening tool for CKD in high-risk individuals.
Agastache rugosa Extract and Its Bioactive Compound Tilianin Suppress Adipogenesis and Lipogenesis on 3T3-L1 Cells
Authors: Hwang Jae Min , Lee Mun-Hoe , Lee Jin-Hee , Lee Jong Hun
Published: August 2021

Agastache rugosa, or Korean mint, is an herb used as a spice, food additive and traditional medicinal ingredient. It has desirable effects, such as its antibacterial, antifungal and antioxidant properties. A. rugosa contains many phenolic compounds studied for their various health benefits, with the primary components being tilianin. A. rugosa extract (ARE), which was extracted with ethanol and freeze-dried, contained 21.14 ± 0.15 mg/g of tilianin with a total polyphenol content of 38.11 ± 0.88 mg/g. Next, the antiadipogenic effect of A. rugosa and tilianin was clarified using 3T3-L1 cells, which differentiate into adipocytes and develop lipid droplets. 3T3-L1 cells were treated with ARE or tilianin and lipid accumulation (%) was calculated through oil red O staining. Tilianin elicited dose-dependent decrease in lipid accumulation (% of positive control) (30 μM 92.10 ± 1.19%; 50 μM 69.25 ± 1.78%; 70 μM 54.86 ± 1.76%; non-differentiation 18.10 ± 0.32%), assessed by oil-red-O staining, whereas ARE treatments caused consistent diminution in lipid accumulation regardless of dose (100 μM 86.90 ± 4.97%; 200 μM 87.25 ± 4.34%; 400 μM 88.54 ± 2.27%; non-differentiation 17.96 ± 1.30%), indicating that both compounds have anti-obesity effects on adipocytes. Treatment with ARE lowered the mRNA (PPARγ; C/EBPα; FABP4; SREBP1; ACC; FAS) and protein (PPARγ; C/EBPα; SREBP1) levels of adipogenesis and lipogenesis-related factors. Tilianin showed a greater effect on the mRNA levels compared with ARE. Thus, tilianin and ARE may have anti-adipogenic and anti-lipogenic effects on 3T3-L1 cells and be possible candidates of obesity-related supplements.
Ozone Activation on TiO2 Studied by IR Spectroscopy and Quantum Chemistry
Authors: Aminev Timur , Krauklis Irina , Pestsov Oleg , Tsyganenko Alexey
Published: August 2021

The adsorption of different isotopic ozone mixtures on TiO2 at 77K was studied using FTIR spectroscopy and DFT calculations of cluster models. In addition to weakly bound ozone with band positions close to those of free or dissolved molecules, the spectrum of chemisorbed species was observed. The splitting of the ν1+3 combination band to eight maxima due to different isotopomers testified to the loss of molecule symmetry. The frequencies of all the isotopic modifications of the ozone molecules which form monodentate or bidentate complexes with four- or five-coordinated titanium atoms were calculated and compared with those of experimentally observed spectra. The four considered complexes adequately reproduced the splitting of the ν1+3 vibration band and the lowered anharmonism of chemisorbed O3. The energetically most favorable monodentate complex with four-coordinated titanium atoms showed good agreement with the observed spectra, although a large difference between the frequencies of ν1 and ν3 modes was found. For better coherence with the experiment, the interaction of the molecule with adjacent cations must be considered.
Rapid Requirements Elicitation of Enterprise Applications Based on Executable Mockups
Authors: Filipović Milorad , Vuković Željko , Dejanović Igor , Milosavljević Gordana
Published: August 2021

Software development begins with the requirements. Misunderstandings with customers in this early phase of development result in wasted development time. This work investigates the possibility of using executable UI mockups in the initial phases of functional requirements elicitation during the development of business applications. Although there has been a lot of research in the field in recent years, we find that there is still a need to improve model-driven tool design in order to enable customer participation from the initial phases of requirement specifications based on working prototypes. These prototypes can directly be reused in the rest of the development process. To meet the goal, we have been developing an open-source solution called Kroki that enables rapid collaborative development. We conducted a series of 10 joint user sessions with domain experts from different domains and backgrounds, resulting in the prototype specifications ranging from 7 to 20 screen mockups accompanied with domain models, developed in two-hour time frames. In this paper, we present our tool design that contributes to rapid joint development, and the results from the user sessions.
Fatigue Characteristics of Double Damage Reinforced Prestressed Hollow Slab Beams under Freeze-Thaw Cycle Erosion
Authors: Zheng Yuanxun , Liu Jiaqi , Guo Pan , Gan Chao
Published: August 2021

A bridge structure is subjected to different external loads and environmental effects during its operation, which results in different types and degrees of damage to the structure during its service life. Reinforcement is often required to maintain regular operation and extend its service life. However, a reinforced bridge structure continues to be subjected to vehicle loads and environmental erosion. Therefore, research on the durability deterioration mechanisms and fatigue life decay of reinforced structures is key to ensuring the long service lives of bridge structures. To study the influence of freeze–thaw cycle erosion on the basic mechanical properties and fatigue characteristics of a bridge structure and a strengthened structure, 2 m long prestressed hollow slab beams were designed and fabricated based on the principle of a similarity ratio and subsequently pre-cracked by fatigue failure. The prestressed hollow slab beams were strengthened after fatigue damage by two methods: pasting steel plates and pasting carbon fiber cloths. After this, a freeze–thaw cycle test was conducted to study the dynamic and static mechanical index changes and the attenuation of the fatigue characteristics of the prestressed strengthened hollow slab beams under freeze–thaw cycle erosion. Meanwhile, a numerical model for reinforced structures was established based on the ABAQUS software to study the mechanisms governing the attenuation of the fatigue life of the prestressed hollow slab beams with different freeze–thaw cycles. The results showed that the deflections and strains observed for the two methods were less than those prior to reinforcement. For instance, the deflection in the span decreased by 14–15%, and the compressive strain decreased by 5.2% to 6%. Under the fatigue load, the prestressed hollow slab beams strengthened by the two methods could withstand a fatigue load cycle of 2 million, and the reinforced components exhibited good fatigue resistance. Under cyclic erosion and fatigue loading, the deflections and strains in the reinforced prestressed hollow slab beams were increased by varying degrees, such as a 30–40% increase in the tensile strain and a 65–70% increase in the span. The fatigue life of the reinforced hollow slab beams decreased with the increasing number of freeze–thaw cycles, and the decay rate of the fatigue life was accelerated.
The Study on Internal Flow Characteristics of Disc Filter under Different Working Condition
Authors: Chi Yanbing , Yang Peiling , Ma Zixuan , Wang Haiying , Liu Yuxuan , Jiang Bingbing , Hu Zongguang
Published: August 2021

A disc filter (DF) is an important component in a micro irrigation system. However, it has a high head loss and low filtration efficiency, which can lead to the inoperability of micro irrigation systems. To improve the filtration ability and to decrease the pressure loss of the irrigation system, it is necessary to internalize the hydraulic characteristics of DFs. In this study, the filter bed of a DF was divided into three parts, i.e., upper, middle, and lower, which were wrapped with a transparent film. The wrapped part was completely blocked. The purpose was to analyze the hydraulic characteristics of different clogged conditions in three types of filters under four types of flows. In addition, we attempted to simulate the filter operation process with computational fluid dynamics, based on two aspects—a macroscopic model and a simplified model. The results showed that the patterns of head loss among all of the DFs was consistent, and the macroscopic model that treated filter bed as a porous medium could express the measured results. The macroscopic models observed that there was a circular flow in the DF, and the flow velocity presented a symmetrical distribution in a horizontal direction. The middle of the filter element appeared in a high-pressure area and demonstrated the highest head loss, which may be the main flow area of the DF, and the inner flow characteristics of the DF were consistent under different conditions. The simplified models showed that the main flow area is near the filter bed in the inner DF, and the flow is tangent to the filter bed between 45 and 90 degrees in a horizontal direction. The uneven distribution of velocity and pressure on the filter bed might be necessary factors to impact filter efficiency.
Applications of Artificial Intelligence in Fire Safety of Agricultural Structures
Authors: Maraveas Chrysanthos , Loukatos Dimitrios , Bartzanas Thomas , Arvanitis Konstantinos G.
Published: August 2021

Artificial intelligence applications in fire safety of agricultural structures have practical economic and technological benefits on commercial agriculture. The FAO estimates that wildfires result in at least USD 1 billion in agriculture-related losses due to the destruction of livestock pasture, destruction of agricultural buildings, premature death of farm animals, and general disruption of agricultural activities. Even though artificial neural networks (ANNs), genetic algorithms (GAs), probabilistic neural networks (PNNs), and adaptive neurofuzzy inference systems (ANFISs), among others, have proven useful in fire prevention, their application is limited in real farm environments. Most farms rely on traditional/non-technology-based methods of fire prevention. The case for AI in agricultural fire prevention is grounded on the accuracy and reliability of computer simulations in smoke movement analysis, risk assessment, and postfire analysis. In addition, such technologies can be coupled with next-generation fire-retardant materials such as intumescent coatings with a polymer binder, blowing agent, carbon donor, and acid donor. Future prospects for AI in agriculture transcend basic fire safety to encompass Society 5.0, energy systems in smart cities, UAV monitoring, Agriculture 4.0, and decentralized energy. However, critical challenges must be overcome, including the health and safety aspects, cost, and reliability. In brief, AI offers unlimited potential in the prevention of fire hazards in farms, but the existing body of knowledge is inadequate.
Wind Characteristics in Mountainous Valleys Obtained through Field Measurement
Authors: Wang Feng , Chen Xinming , He Rui , Liu Yan , Hao Jianming , Li Jiawu
Published: August 2021

The wind characteristics of the mountainous environment in Western China are extremely complex. Many advances have occurred in the study of wind characteristics in plains or hilly areas. However, some limitations remain in the study of mountain passes and riverbanks. We selected a mountain pass and river beach in a certain area as the research objects, then set up observation instruments at different locations to establish temporary observation stations to collect more than one year of measured data. By processing the measurement data during the observation period, the mean wind characteristics and fluctuating wind characteristics of the area were obtained. Through comparison with the standard, we found strong nonstationary characteristics of the wind, such as large deviations between the wind field characteristics values in the standard and measured values, proving the need for wind field observations of mountain passes and riverbanks.
ZFTool: A Software for Automatic Quantification of Cancer Cell Mass Evolution in Zebrafish
Authors: Carreira María J. , Vila-Blanco Nicolás , Cabezas-Sainz Pablo , Sánchez Laura
Published: August 2021

Background: Zebrafish (Danio rerio) is a model organism for the study of human cancer. Compared with the murine model, the zebrafish model has several properties ideal for personalized therapies. The transparency of the zebrafish embryos and the development of the pigment-deficient ”casper“ zebrafish line give the capacity to directly observe cancer formation and progression in the living animal. Automatic quantification of cellular proliferation in vivo is critical to the development of personalized medicine. Methods: A new methodology was defined to automatically quantify the cancer cellular evolution. ZFTool was developed to establish a base threshold that eliminates the embryo autofluorescence, automatically measures the area and intensity of GFP (green-fluorescent protein) marked cells, and defines a proliferation index. Results: The proliferation index automatically computed on different targets demonstrates the efficiency of ZFTool to provide a good automatic quantification of cancer cell evolution and dissemination. Conclusion: Our results demonstrate that ZFTool is a reliable tool for the automatic quantification of the proliferation index as a measure of cancer mass evolution in zebrafish, eliminating the influence of its autofluorescence.
Analysis of the Dynamic Stiffness, Hysteresis Resonances and Complex Responses for Nonlinear Spring Systems in Power-Form Order
Authors: Wang Qingtao , Zhang Zhiyong , Ying Yongheng , Pang Zhaojun
Published: August 2021

Power-form nonlinear contact force models are widely adopted in relatively moving parts of macro (e.g., rolling bearings considering Hertzian contact restoring force between rolling elements and bearing raceways) or micro (e.g., the micro cantilever probe system of atomic force microscopy) scale mechanical systems, and contact resonance could cause serious problems of wear, contact fatigue, vibration, and noise, which has attracted widespread attention. In the present paper, the softening/hardening stiffness characteristics of continuous and one-sided contact power-form nonlinear spring models are addressed, respectively, by the analysis of the monotone features of resonant frequency-response skeleton lines. Herein, the period-n solution branch and its stability characteristics are obtained by the harmonic balance and alternating frequency/time domain (HB–AFT) method and Floquet theory. Compared with previous studies, this paper will furtherly clarify the influences of externally normal load, the power form exponent term, and excitation amplitude on the softening/hardening stiffness characteristics of general power-form spring systems. In addition, for a power-form system with a one-sided contact, the phenomena of primary and super/sub-harmonic hysteretic resonances inducing period-doubling, folding bifurcation, the coexistence of multiple solutions are demonstrated. Besides, it gives the evolution mechanism of two types of intermittency chaos in a one-sided contact system. The overall results may have certain basic theoretical significance and engineering values for the control of vibration and noise in contact mechanical systems.
Carbon Dioxide Adsorption on Carbon Nanofibers with Different Porous Structures
Authors: Chiang Yu-Chun , Huang Chih-Cheng , Chin Wei-Ting
Published: August 2021

Electrospinning techniques have become an efficient way to produce continuous and porous carbon nanofibers. In view of CO2 capture as one of the important works for alleviating global warming, this study intended to synthesize polyacrylonitrile (PAN)-based activated carbon nanofibers (ACNFs) using electrospinning processes for CO2 capture. Different structures of PAN-based ACNFs were prepared, including solid, hollow, and porous nanofibers, where poly(methyl methacrylate) (PMMA) was selected as the sacrificing core or pore generator. The results showed that the PMMA could be removed successfully at a carbonization temperature of 900 °C, forming the hollow or porous ACNFs. The diameters of the ACNFs ranged from 500 to 900 nm, and the shell thickness of the hollow ACNFs was approximately 70–110 nm. The solid ACNFs and hollow ACNFs were microporous materials, while the porous ACNFs were characterized by hierarchical pore structures. The hollow ACNFs and porous ACNFs possessed higher specific surface areas than that of the solid ACNFs, while the solid ACNFs exhibited the highest microporosity (94%). The CO2 adsorption capacity on the ACNFs was highly dependent on the ratio of V<0.7 nm to Vt, the ratio of Vmi to Vt, and the N-containing functional groups. The CO2 adsorption breakthrough curves could be curve-fitted well with the Yoon and Nelson model. Furthermore, the 10 cyclic tests demonstrated that the ACNFs are promising adsorbents.
Radiation-Induced Stable Radicals in Calcium Phosphates: Results of Multifrequency EPR, EDNMR, ESEEM, and ENDOR Studies
Authors: Murzakhanov Fadis F. , Grishin Peter O. , Goldberg Margarita A. , Yavkin Boris V. , Mamin Georgy V. , Orlinskii Sergei B. , Fedotov Alexander Yu. , Petrakova Natalia V. , Antuzevics Andris , Gafurov Marat R. , Komlev Vladimir S.
Published: August 2021

This article presents the results of a study of radiation-induced defects in various synthetic calcium phosphate (CP) powder materials (hydroxyapatite—HA and octacalcium phosphate—OCP) by electron paramagnetic resonance (EPR) spectroscopy at the X, Q, and W-bands (9, 34, 95 GHz for the microwave frequencies, respectively). Currently, CP materials are widely used in orthopedics and dentistry owing to their high biocompatibility and physico-chemical similarity with human hard tissue. It is shown that in addition to the classical EPR techniques, other experimental approaches such as ELDOR-detected NMR (EDNMR), electron spin echo envelope modulation (ESEEM), and electron-nuclear double resonance (ENDOR) can be used to analyze the electron–nuclear interactions of CP powders. We demonstrated that the value and angular dependence of the quadrupole interaction for 14N nuclei of a nitrate radical can be determined by the EDNMR method at room temperature. The ESEEM technique has allowed for a rapid analysis of the nuclear environment and estimation of the structural positions of radiation-induced centers in various crystal matrices. ENDOR spectra can provide information about the distribution of the nitrate radicals in the OCP structure.
An Experimental Study of Nailed Soil Slope Models: Effects of Building Foundation and Soil Characteristics
Authors: Mohamed Mahmoud , Ahmed Mohd , Mallick Javed
Published: August 2021

A soil nailing system is a proven effective and economic method used to stabilize earth slopes from the external (factors increasing the shear stress) and internal (factors decreasing material strength) failure causes. The laboratory models with scales of 1:10 are used to study the behavior of nailed soil slope with different soil and building foundation parameters. The models consist of Perspex strips as facing and steel bars as a nailing system to increase the stability of the soil slope. The models of sand beds are formed using an automatic sand raining system. Devices and instruments are installed to monitor the behavior of soil-nailed slope during and after construction. The effect of the soil type, soil slope angle, foundation width and position on the force mobilized in the nail, lateral displacement of the slope, settlement of the foundation and the earth pressure at the slope face, under and behind the soil mass at various foundation pressures, has been observed. It is found that the increase of soil density reduces both slopes facing displacement and building foundation settlements. The slope face displacement and footing settlement will increase with an increase in the width of the foundation and foundation position near the crest of the slope.
The Impact of Geographical Location on the Chemical Compositions of Pimpinella lutea Desf. Growing in Tunisia
Authors: AL-Hmadi Hekmat , El Mokni Ridha , Joshi Rajesh K. , Ashour Mohamed L. , Hammami Saoussen
Published: August 2021

Essential oils are generally produced to confer the protection of medicinal plants against several natural enemies. Variations of chemical and physical environmental factors exert significant influences on plant development. They hence may affect the quality and quantity of volatile organic metabolites of interest and, therefore, the economic applications of essential oils. This research focused on the effects of the harvest region on the production and analytes present in Tunisian Pimpinella lutea Desf. Apiaceae that were collected in three different growing environments (North and South Bizerta and Tabarka). Essential oils extracted from a variety of genotypes were analyzed, for the first time, using gas chromatography and mass spectrometry (GC/FID and GC/MS). The determination of the percentage of essential oil components allowed the recognition of three chemotypes: α-trans-Bergamotene quantified at a percentage of 18.1% in North Bizerta (NBEO), muurola-4,10(14)-dien-1-β-ol identified in South Bizerta (10.1%, SBEO) and acora-3,7(14)-dien present in a high level of 29.1% in Tabarka population (TEO). The richness of different populations in sesquiterpenes (60.2–78.1%) suggests that Pimpinella lutea Desf. may be used in different industrial segments.
Visual MAV Tracker with Adaptive Search Region
Authors: Park Wooryong , Lee Donghee , Yi Junhak , Nam Woochul
Published: August 2021

Tracking a micro aerial vehicle (MAV) is challenging because of its small size and swift motion. A new model was developed by combining compact and adaptive search region (SR). The model can accurately and robustly track MAVs with a fast computation speed. A compact SR, which is slightly larger than a target MAV, is less likely to include a distracting background than a large SR; thus, it can accurately track the MAV. Moreover, the compact SR reduces the computation time because tracking can be conducted with a relatively shallow network. An optimal SR to MAV size ratio was obtained in this study. However, this optimal compact SR causes frequent tracking failures in the presence of the dynamic MAV motion. An adaptive SR is proposed to address this problem; it adaptively changes the location and size of the SR based on the size, location, and velocity of the MAV in the SR. The compact SR without adaptive strategy tracks the MAV with an accuracy of 0.613 and a robustness of 0.086, whereas the compact and adaptive SR has an accuracy of 0.811 and a robustness of 1.0. Moreover, online tracking is accomplished within approximately 400 frames per second, which is significantly faster than the real-time speed.
Dropped Object Impact Analysis Considering Frequency and Consequence for LNG-FPSO Topside Module
Authors: Lee Kwangkook , Ryu Hyunsu
Published: August 2021

Recently, quantitative risk assessment (QRA) has been widely used as a decision-making tool in the offshore industry. This study focused on analyzing dropped objects in the design of a modern offshore platform. A modified QRA procedure was developed for assessing production module protection against accidental external loads. Frequency and consequence analyses were performed using the developed QRA procedure. An exceedance curve was plotted, and a high-risk management item was derived through this process. In particular, simulations and experiments were used to verify the difference between the potential and impact energies according to drop orientation. When the object dropped in a specific orientation, the impact energy was confirmed to be up to 4.7 times greater than the potential energy. To reflect the QRA results in structural design, the proposed procedure should be used to calculate the maximum impact energy. The proposed procedure provides a step-by-step guide to assess the damage capacity of a production area as well as the damage frequency and consequences.
The Threshold between the Material and Immaterial Light. Notes on Luminescence and Dark Environments
Authors: Rocha Ana Margarida , Almeida Teresa , Machado Graciela
Published: August 2021

This article discusses the phenomenon of luminescence in the production and visualization of images from an art-practice standpoint. The theoretical argument is developed through an analysis of artistic work that explores, inserts, expands, articulates, and interrogates the internal contradictions of UV light and the transitivity of light-sensitive materials in installation contexts. This investigation explores complexities in the encounter of antagonistic concepts: the threshold phenomena between materiality and immateriality, visibility and invisibility, light and darkness, disclosure and concealment. It aims to articulate a new perspective on contemporary debates on physiological, psychological, and environmental effects of light and darkness, articulated through aesthetic experience and artistic practice. Methods for engaging in the sensation of light and darkness will be introduced and how it unfolds as experiential qualities within installation projects will be considered.
Variations in the Peczely Macro-Synoptic Types (1881–2020) with Attention to Weather Extremes in the Pannonian Basin
Authors: Mika János , Károssy Csaba , Lakatos László
Published: August 2021

Daily Peczely circulation types are investigated over 140 years (1881–2020). After presenting monthly mean frequencies and durations of the 13 circulation types, two further questions are investigated: (i) How do the circulation types influence local weather extremes?; (ii) Are there significant trends in the frequency of the original and the grouped circulation types in the recent monotonically warming 50 year period (1971–2020)? The answers are as follows: (i) Four local weather extremes were investigated in nine grid-points of the Pannonian Basin and analyzed in the central months of the seasons. It was established that high precipitation and wind maxima occur in almost all circulation types and months, whereas for both high temperature maxima and low temperature minima, there are six circulation types, where no extremity occurred in one, two, or three investigated months. (ii) In the last 50 years, 37% of the linear seasonal frequency trends have been significant. However, these trends are rarely significant in the shorter monotonously warming (1911–1940) and cooling (1941–1970) 30-year periods. Therefore, the significant trends of the last 50 years are unlikely to be the direct consequences of the monotonous hemispherical warming. Since these hemispherical temperature trends are most likely caused by different sets of physical reasons, the reality of the presented circulation frequency trends needs to be validated by climate models.
The Optimization Strategies on Clarification of the Misconceptions of Big Data Processing in Dynamic and Opportunistic Environments
Authors: Li Wei , Tang Maolin
Published: August 2021

This paper identifies four common misconceptions about the scalability of volunteer computing on big data problems. The misconceptions are then clarified by analyzing the relationship between scalability and the impact factors including the problem size of big data, the heterogeneity and dynamics of volunteers, and the overlay structure. This paper proposes optimization strategies to find the optimal overlay for the given big data problem. This paper forms multiple overlays to optimize the performance of individual steps in terms of MapReduce paradigm. The optimization is to achieve the maximum overall performance by using a minimum number of volunteers, not overusing resources. This paper has demonstrated that the simulations on the concerned factors can fast find the optimization points. This paper concludes that always welcoming more volunteers is an overuse of available resources because they do not always bring benefit to the overall performance. Finding optimal use of volunteers are possible for the given big data problems even on the dynamics and opportunism of volunteers.
Gastroprotection against Rat Ulcers by Nephthea Sterol Derivative
Authors: Mohamed Tarik A. , Elshamy Abdelsamed I. , Ibrahim Mahmoud A. A. , Atia Mohamed A. M. , Ahmed Rania F. , Ali Sherin K. , Mahdy Karam A. , Alshammari Shifaa O. , Al-Abd Ahmed M. , Moustafa Mahmoud F. , Farrag Abdel Razik H. , Hegazy Mohamed-Elamir F.
Published: August 2021

Different species belonging to the genus Nephthea (Acyonaceae) are a rich resource for bioactive secondary metabolites. The literature reveals that the gastroprotective effects of marine secondary metabolites have not been comprehensively studied in vivo. Hence, the present investigation aimed to examine and determine the anti-ulcer activity of 4α,24-dimethyl-5α-cholest-8β,18-dihydroxy,22E-en-3β-ol (ST-1) isolated from samples of a Nephthea species. This in vivo study was supported by in silico molecular docking and protein–protein interaction techniques. Oral administration of ST-1 reduced rat stomach ulcers with a concurrent increase in gastric mucosa. Molecular docking calculations against the H+/K+-ATPase transporter showed a higher binding affinity of ST-1, with a docking score value of −9.9 kcal/mol and a pKi value of 59.7 nM, compared to ranitidine (a commercial proton pump inhibitor, which gave values of −6.2 kcal/mol and 27.9 µM, respectively). The combined PEA-reactome analysis results revealed promising evidence of ST-1 potency as an anti-ulcer compound through significant modulation of the gene set controlling the PI3K signaling pathway, which subsequently plays a crucial role in signaling regarding epithelialization and tissue regeneration, tissue repairing and tissue remodeling. These results indicate a probable protective role for ST-1 against ethanol-induced gastric ulcers.
3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink
Authors: Restan Perez Milena , Sharma Ruchi , Masri Nadia Zeina , Willerth Stephanie Michelle
Published: August 2021

Current treatments for neurodegenerative diseases aim to alleviate the symptoms experienced by patients; however, these treatments do not cure the disease nor prevent further degeneration. Improvements in current disease-modeling and drug-development practices could accelerate effective treatments for neurological diseases. To that end, 3D bioprinting has gained significant attention for engineering tissues in a rapid and reproducible fashion. Additionally, using patient-derived stem cells, which can be reprogrammed to neural-like cells, could generate personalized neural tissues. Here, adipose tissue-derived mesenchymal stem cells (MSCs) were bioprinted using a fibrin-based bioink and the microfluidic RX1 bioprinter. These tissues were cultured for 12 days in the presence of SB431542 (SB), LDN-193189 (LDN), purmorphamine (puro), fibroblast growth factor 8 (FGF8), fibroblast growth factor-basic (bFGF), and brain-derived neurotrophic factor (BDNF) to induce differentiation to dopaminergic neurons (DN). The constructs were analyzed for expression of neural markers, dopamine release, and electrophysiological activity. The cells expressed DN-specific and early neuronal markers (tyrosine hydroxylase (TH) and class III beta-tubulin (TUJ1), respectively) after 12 days of differentiation. Additionally, the tissues exhibited immature electrical signaling after treatment with potassium chloride (KCl). Overall, this work shows the potential of bioprinting engineered neural tissues from patient-derived MSCs, which could serve as an important tool for personalized disease models and drug-screening.