bluuebunny/crossref_metadata_2025 huggingface dataset:
The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene
Authors: Li Charng-Pei , Wu Dong-Hong , Huang Shou-Horng , Meng Menghsiao , Shih Hsien-Tzung , Lai Ming-Hsin , Chen Liang-Jwu , Jena Kshirod K. , Hechanova Sherry Lou , Ke Ting-Jyun , Chiu Tai-Yuan , Tsai Zong-Yuan , Chen Guo-Kai , Tsai Kuan-Chieh , Leu Wei-Ming
Published: January 2023

Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice.
An Overview of Circulating Cell-Free Nucleic Acids in Diagnosis and Prognosis of Triple-Negative Breast Cancer
Authors: Tierno Domenico , Grassi Gabriele , Zanconati Fabrizio , Bortul Marina , Scaggiante Bruna
Published: January 2023

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer due to its molecular heterogeneity and poor clinical outcomes. Analysis of circulating cell-free tumor nucleic acids (ctNAs) can improve our understanding of TNBC and provide efficient and non-invasive clinical biomarkers that may be representative of tumor heterogeneity. In this review, we summarize the potential of ctNAs to aid TNBC diagnosis and prognosis. For example, tumor fraction of circulating cell-free DNA (TFx) may be useful for molecular prognosis of TNBC: high TFx levels after neoadjuvant chemotherapy have been associated with shorter progression-free survival and relapse-free survival. Mutations and copy number variations of TP53 and PIK3CA/AKT genes in plasma may be important markers of TNBC onset, progression, metastasis, and for clinical follow-up. In contrast, the expression profile of circulating cell-free tumor non-coding RNAs (ctncRNAs) can be predictive of molecular subtypes of breast cancer and thus aid in the identification of TBNC. Finally, dysregulation of some circulating cell-free tumor miRNAs (miR17, miR19a, miR19b, miR25, miR93, miR105, miR199a) may have a predictive value for chemotherapy resistance. In conclusion, a growing number of efforts are highlighting the potential of ctNAs for future clinical applications in the diagnosis, prognosis, and follow-up of TNBC.
Noncoding RNAs: Master Regulator of Fibroblast to Myofibroblast Transition in Fibrosis
Authors: Zhang Huamin , Zhou Yutong , Wen Dada , Wang Jie
Published: January 2023

Myofibroblasts escape apoptosis and proliferate abnormally under pathological conditions, especially fibrosis; they synthesize and secrete a large amount of extracellular matrix (ECM), such as α-SMA and collagen, which leads to the distortion of organ parenchyma structure, an imbalance in collagen deposition and degradation, and the replacement of parenchymal cells by fibrous connective tissues. Fibroblast to myofibroblast transition (FMT) is considered to be the main source of myofibroblasts. Therefore, it is crucial to explore the influencing factors regulating the process of FMT for the prevention, treatment, and diagnosis of FMT-related diseases. In recent years, non-coding RNAs, including microRNA, long non-coding RNAs, and circular RNAs, have attracted extensive attention from scientists due to their powerful regulatory functions, and they have been found to play a vital role in regulating FMT. In this review, we summarized ncRNAs which regulate FMT during fibrosis and found that they mainly regulated signaling pathways, including TGF-β/Smad, MAPK/P38/ERK/JNK, PI3K/AKT, and WNT/β-catenin. Furthermore, the expression of downstream transcription factors can be promoted or inhibited, indicating that ncRNAs have the potential to be a new therapeutic target for FMT-related diseases.
Identification of FGF13 as a Potential Biomarker and Target for Diagnosis of Impaired Glucose Tolerance
Authors: Chen Qi , Li Fangyu , Gao Yuanyuan , Yang Fengying , Yuan Li
Published: January 2023

Early identification of pre-diabetes provides an opportunity for intervention and treatment to delay its progression to type 2 diabetes mellitus (T2DM). We aimed to identify the biomarkers of impaired glucose tolerance (IGT) through bioinformatics analysis. The GSE76896 dataset, including non-diabetic (ND), IGT, and T2DM clinical samples, was deeply analyzed to identify 309 Co-DEGs for IGT and T2DM. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that inflammatory responses and the PI3K-AKT signaling pathway are important patho-physiological features of IGT and T2DM. Protein–protein interaction (PPI) network analysis and cytoHubba technolgy identified seven hub genes: namely, CCL2, CXCL1, CXCL8, EDN1, FGF13, MMP1, and NGF. The expression and ROC curves of these hub genes were validated using the GSE38642 dataset. Through an immunofluorescence assay, we found that the expression of FGF13 in islets of mice in the HFD and T2DM groups was significantly lower than in the control group. Similarly, the level of FGF13 in the sera of IGT and T2DM patients was lower than that in the healthy group. Together, these results suggest that FGF13 can be treated as a novel biomarker of IGT, which may provide new targets for the diagnosis and treatment of pre-diabetes and T2DM.
Spatial Transcriptome Profiling of Mouse Hippocampal Single Cell Microzone in Parkinson’s Disease
Authors: Jia Erteng , Sheng Yuqi , Shi Huajuan , Wang Ying , Zhou Ying , Liu Zhiyu , Qi Ting , Pan Min , Bai Yunfei , Zhao Xiangwei , Ge Qinyu
Published: January 2023

The hippocampus is an important part of the limbic system in the human brain that has essential roles in spatial navigation and cognitive functions. It is still unknown how gene expression changes in single-cell in different spatial locations of the hippocampus of Parkinson’s disease. The purpose of this study was to analyze the gene expression features of single cells in different spatial locations of mouse hippocampus, and to explore the effects of gene expression regulation on learning and memory mechanisms. Here, we obtained 74 single-cell samples from different spatial locations in a mouse hippocampus through microdissection technology, and used single-cell RNA-sequencing and spatial transcriptome sequencing to visualize and quantify the single-cell transcriptome features of tissue sections. The results of differential expression analysis showed that the expression of Sv2b, Neurod6, Grp and Stk32b genes in a hippocampus single cell at different locations was significantly different, and the marker genes of CA1, CA3 and DG subregions were identified. The results of gene function enrichment analysis showed that the up-regulated differentially expressed genes Tubb2a, Eno1, Atp2b1, Plk2, Map4, Pex5l, Fibcd1 and Pdzd2 were mainly involved in neuron to neuron synapse, vesicle-mediated transport in synapse, calcium signaling pathway and neurodegenerative disease pathways, thus affecting learning and memory function. It revealed the transcriptome profile and heterogeneity of spatially located cells in the hippocampus of PD for the first time, and demonstrated that the impaired learning and memory ability of PD was affected by the synergistic effect of CA1 and CA3 subregions neuron genes. These results are crucial for understanding the pathological mechanism of the Parkinson’s disease and making precise treatment plans.
Recent Studies of Artificial Intelligence on In Silico Drug Distribution Prediction
Authors: Tran Thi Tuyet Van , Tayara Hilal , Chong Kil To
Published: January 2023

Drug distribution is an important process in pharmacokinetics because it has the potential to influence both the amount of medicine reaching the active sites and the effectiveness as well as safety of the drug. The main causes of 90% of drug failures in clinical development are lack of efficacy and uncontrolled toxicity. In recent years, several advances and promising developments in drug distribution property prediction have been achieved, especially in silico, which helped to drastically reduce the time and expense of screening undesired drug candidates. In this study, we provide comprehensive knowledge of drug distribution background, influencing factors, and artificial intelligence-based distribution property prediction models from 2019 to the present. Additionally, we gathered and analyzed public databases and datasets commonly utilized by the scientific community for distribution prediction. The distribution property prediction performance of five large ADMET prediction tools is mentioned as a benchmark for future research. On this basis, we also offer future challenges in drug distribution prediction and research directions. We hope that this review will provide researchers with helpful insight into distribution prediction, thus facilitating the development of innovative approaches for drug discovery.
The Black Box Orchestra of Gut Bacteria and Bile Acids: Who Is the Conductor?
Authors: Majait Soumia , Nieuwdorp Max , Kemper Marleen , Soeters Maarten
Published: January 2023

Over the past decades the potential role of the gut microbiome and bile acids in type 2 diabetes mellitus (T2DM) has been revealed, with a special reference to low bacterial alpha diversity. Certain bile acid effects on gut bacteria concern cytotoxicity, or in the case of the microbiome, bacteriotoxicity. Reciprocally, the gut microbiome plays a key role in regulating the bile acid pool by influencing the conversion and (de)conjugation of primary bile acids into secondary bile acids. Three main groups of bacterial enzymes responsible for the conversion of bile acids are bile salt hydrolases (BSHs), hydroxysteroid dehydrogenases (HSDHs) and enzymes encoded in the bile acid inducible (Bai) operon genes. Interventions such as probiotics, antibiotics and fecal microbiome transplantation can impact bile acids levels. Further evidence of the reciprocal interaction between gut microbiota and bile acids comes from a multitude of nutritional interventions including macronutrients, fibers, prebiotics, specific individual products or diets. Finally, anatomical changes after bariatric surgery are important because of their metabolic effects. The heterogeneity of studies, diseases, bacterial species and (epi)genetic influences such as nutrition may challenge establishing specific and detailed interventions that aim to tackle the gut microbiome and bile acids.
Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen
Authors: Zrelovs Nikita , Jansons Juris , Kazaka Tatjana , Kazaks Andris , Dislers Andris
Published: January 2023

The bacterial genus Pantoea comprises species found in a variety of different environmental sources. Pantoea spp. are often recovered from plant material and are capable of both benefitting the plants and acting like phytopathogens. Some species of Pantoea (including P. agglomerans) are considered opportunistic human pathogens capable of causing various infections in immunocompromised subjects. In this study, a strain of P. agglomerans (identified by 16S rRNA gene sequencing) was isolated from a dead specimen of an unidentified Latvian grasshopper species. The retrieved strain of P. agglomerans was then used as a host for the potential retrieval of phages from the same source material. After rounds of plaque purification and propagation, three high-titer lysates corresponding to putatively distinct phages were acquired. Transmission electron microscopy revealed that one of the phages was a myophage with an unusual morphology, while the two others were typical podophages. Whole-genome sequencing (WGS) was performed for each of these isolated phages. Genome de novo assembly and subsequent functional annotation confirmed that three different strictly lytic phages were isolated. Elaborate genomic characterization of the acquired phages was performed to elucidate their place within the so-far-uncovered phage diversity.
Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials
Authors: Lyagin Ilya , Stepanov Nikolay , Presnov Denis , Trifonov Artem , Efremenko Elena
Published: January 2023

The self-assembling of nanosized materials is a promising field for research and development. Multiple approaches are applied to obtain inorganic, organic and composite nanomaterials with different functionality. In the present work, self-assembling nanocomplexes (NCs) were prepared on the basis of enzymes and polypeptides followed by the investigation of the influence of low-molecular weight biologically active compounds on the properties of the NCs. For that, the initially possible formation of catalytically active self-assembling NCs of four hydrolytic enzymes with nine effectors was screened via molecular modeling. It allowed the selection of two enzymes (hexahistidine-tagged organophosphorus hydrolase and penicillin acylase) and two compounds (emodin and naringenin) having biological activity. Further, such NCs based on surface-modified enzymes were characterized by a batch of physical and biochemical methods. At least three NCs containing emodin and enzyme (His6-OPH and/or penicillin acylase) have been shown to significantly improve the antibacterial activity of colistin and, to a lesser extent, polymyxin B towards both Gram-positive bacteria (Bacillus subtilis) and Gram-negative bacteria (Escherichia coli).
Acknowledgment to the Reviewers of International Journal of Plant Biology in 2022
Authors:   International Journal of Plant Biology Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
Risk Factors Involved in the High Incidence of Bladder Cancer in an Industrialized Area in North-Eastern Spain: A Case–Control Study
Authors: Caballero José M. , Gili José M. , Pereira Juan C. , Gomáriz Alba , Castillo Carlos , Martín-Baranera Montserrat
Published: January 2023

Bladder cancer (BC) is the most common of the malignancies affecting the urinary tract. Smoking and exposure to occupational and environmental carcinogens are responsible for most cases. Vallès Occidental is a highly industrialized area in north-eastern Spain with one of the highest incidences of BC in men. We carried out a case–control study in order to identify the specific risk factors involved in this area. Three hundred and six participants were included (153 cases BC and 153 controls matched for age and sex): in each group, 89.5% (n = 137) were male and the mean age was 71 years (range 30–91; SD = 10.6). There were no differences between groups in family history, body mass index, or dietary habits. Independent risk factors for CV were smoking (OR 2.08; 95% CI 1.30–3.32; p = 0.002), the use of analgesics in nonsmokers (OR 10.00; 95% CI 1.28–78.12; p = 0.028), and profession (OR: 8.63; 95% CI 1.04–71.94; p = 0.046). The consumption of black and blond tobacco, the use of analgesics in nonsmokers, and occupational exposures are risk factors for the development of BC in this area, despite the reduction in smoking in the population and the extensive measures taken in the last few decades in major industries to prevent exposure to occupational carcinogens.
Gallium-Doped Hydroxyapatite Shows Antibacterial Activity against Pseudomonas aeruginosa without Affecting Cell Metabolic Activity
Authors: Mosina Marika , Siverino Claudia , Stipniece Liga , Sceglovs Artemijs , Vasiljevs Renats , Moriarty T. Fintan , Locs Janis
Published: January 2023

Calcium phosphates (CaPs) have been used in bone regeneration for decades. Among the described CaPs, synthetic hydroxyapatite (HAp) has a chemical composition similar to that of natural bone. Gallium-containing compounds have been studied since the 1970s for the treatment of autoimmune diseases and have shown beneficial properties, such as antibacterial activity and inhibition of osteoclast activity. In this study, we synthesized hydroxyapatite (HAp) powder with Ga doping ratios up to 6.9 ± 0.5 wt% using the wet chemical precipitation method. The obtained products were characterized using XRD, BET, FTIR, and ICP-MS. Ga3+ ion release was determined in the cell culture media for up to 30 days. Antibacterial activity was assessed against five bacterial species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes. The biocompatibility of the GaHAp samples was determined in human fibroblasts (hTERT-BJ1) through direct and indirect tests. The structure of the synthesized products was characteristic of HAp, as revealed with XRD and FTIR, although the addition of Ga caused a decrease in the crystallite size. Ga3+ was released from GaHAp paste in a steady manner, with approximately 40% being released within 21 days. GaHAp with the highest gallium contents, 5.5 ± 0.1 wt% and 6.9 ± 0.5 wt%, inhibited the growth of all five bacterial species, with the greatest activity being against Pseudomonas aeruginosa. Biocompatibility assays showed maintained cell viability (~80%) after seven days of indirect exposure to GaHAp. However, when GaHAp with Ga content above 3.3 ± 0.4 wt% was directly applied on the cells, a decrease in metabolic activity was observed on the seventh day. Overall, these results show that GaHAp with Ga content below 3.3 ± 0.4 wt% has attractive antimicrobial properties, without affecting the cell metabolic activity, creating a material that could be used for bone regeneration and prevention of infection.
Acknowledgment to the Reviewers of Journal of Molecular Pathology in 2022
Authors:   JMP Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
Behaviour and Microstructural Characteristics of Lime-GGBS-Treated Kaolin Clay Contaminated with Gypsum
Authors: Jeremiah Jeremiah J. , Abbey Samuel J. , Booth Colin A. , Kashyap Anil
Published: January 2023

In this experimental study, the physico-mechanical and microstructural properties of sulphate-bearing clays have been investigated. Sulphate bearing soils constituted by mixing kaolin and gypsum at 0%, 15%, 25%, and 35% gypsum contents were treated with 12% ordinary Portland cement (OPC) and 4%Lime (L) and 8% ground granulated blast furnace slag (GGBS) and subjected to compaction, swell, unconfined compressive strength (UCS), California bearing ratio (CBR), and scanning electron microscopy (SEM) and energy dispersive spectrometry (EDX) analyses. The results of the study showed that the use of L-GGBS improved the soaked CBRs of the treated samples by over 43% when compared to OPC-treated samples after 7-days curing. A reduction in water absorption by 82% was also observed with L-GGBS treatment after 28-days curing. The UCS results also showed better performance with L-GGBS treatment exceeding 856% at 28 days. The effect of increased cementitious product with increasing gypsum content was negated by simultaneous and rapid growth of ettringite minerals which reduced the strength and increased swelling of OPC treated samples up to 18.92%, exceeding allowable limits of 2.5% as specified in Highway Agency Advice Note HA 74/07. The L-GGBS treated gypseous soil samples meet the strength requirement for stabilised sub-base (CS) and stabilised road-bases (CB1 and CB2) as described in TRL ORN31. Hence, the use of L-GGBS combination was found to be effective in ameliorating sulphate-induced expansion and therefore encouraged in the stabilisation of subgrade and road-base materials with high sulphate contents.
Hydro- and Icephobic Properties and Durability of Epoxy Gelcoat Modified with Double-Functionalized Polysiloxanes
Authors: Ziętkowska Katarzyna , Kozera Rafał , Przybyszewski Bartłomiej , Boczkowska Anna , Sztorch Bogna , Pakuła Daria , Marciniec Bogdan , Przekop Robert Edward
Published: January 2023

Anti-icing coatings have provided a very good alternative to current, uneconomic, active deicing methods, and their use would bring a number of significant benefits to many industries, such as aviation and energy. Some of the most promising icephobic surfaces are those with hydrophobic properties. However, the relationship between hydrophobicity and low ice adhesion is not yet clearly defined. In this work, chemical modification of an epoxy gelcoat with chemical modifiers from the group of double organofunctionalized polysiloxanes (generally called multifunctionalized organosilicon compounds (MFSCs)) was applied. The anti-icing properties of manufactured coatings were determined by means of measurements of shear strength between the ice layer and the modified surface, conducted using a tensile machine. In the work, tests were also performed on the roughness, wettability, and durability of the properties in an aging chamber. It was found that the performed modifications of the coating’s chemical composition by the addition of polysiloxanes enabled us to reduce ice adhesion by 51% and to increase the water contact angle by 14% in comparison to the neat gelcoat. A reduction in ice adhesion was also observed with the increasing water contact angle and with decreasing surface roughness. In addition, only one modification recorded an increase in ice adhesion after exposure in the aging chamber.
The Effect of Fe/Al Ratio and Substrate Hardness on Microstructure and Deposition Behavior of Cold-Sprayed Fe/Al Coatings
Authors: Wang You , Deng Nan , Tong Zhenfeng , Zhou Zhangjian
Published: January 2023

Fe/Al composite coatings with compositions of Fe-25 wt.% Al, Fe-50 wt.% Al and Fe-75 wt.% Al were deposited on pure Al and P91 steel plates by a cold spray, respectively. The microstructure of the cross-section of the fabricated coatings was characterized by SEM and EDX. The bonding strength between the coatings and substrates was measured and analyzed. The effects of the Fe/Al ratios and substrate hardness on the deposition behavior were investigated. It was interesting to find fragmented zones in all fabricated coatings, which were composed of large integrated Al particles and small fragmented Al particles. Meanwhile, the fraction of fragmented zones varied with the fraction of the actual Fe/Al ratio. An Fe/Al ratio of 50/50 appeared to be an optimized ratio for the higher bonding strength of coatings. The in situ hammer effect caused by larger and harder Fe particles played an important role in the cold spray process. The substrate with the higher hardness strengthened the in situ hammer effect and further improved the bonding strength.
Testing and Analysis of Uniaxial Mechanical Fatigue, Charpy Impact Fracture Energy and Microhardness of Two Low-Carbon Steels
Authors: Brnic Josip , Balos Sebastian , Brcic Marino , Dramicanin Miroslav , Krscanski Sanjin , Milutinovic Mladomir , Ding Biao , Gao Zeng
Published: January 2023

The paper presents and analyzes the results of experimental tests performed on two non-alloy low carbon steels (1.1141 and 1.0122) in cases of their exposure to impact fracture energy and uniaxial high cyclic mechanical stress-controlled fatigue. The experimental results provide insight into the changes in the Charpy impact fracture energy of the V-notched test specimen that occur as a result of temperature changes. The experimental results also provide insight into the mechanical response of the tested materials to mechanical uniaxial high-cycle fatigue at room temperature in an air atmosphere and at different applied stress ratios. Material fatigue tests refer to symmetric (R = −1), asymmetric (R = −0.5) and pulsating tensile (R = 0) cycles. The test results are shown in the S–N diagrams and refer to the highest applied stresses in relation to the number of failures at a given stress ratio. Using the modified staircase method, the fatigue limit (endurance limit) was calculated for both tested materials at each prescribed stress ratio. For both tested steel alloys, and at prescribed stress ratios, the fatigue limit levels (σ_f) are shown as follows: for steel C15E+C (1.1141)→σf[250.8R=−1; 345.4R=−0.5; 527R=0](MPa); and for steel S235JRC+C (1.0122)→σf[202R=−1; 310R=−0.5; 462R=0](MPa). All uniaxial fatigue tests were performed on unnotched, smooth, highly-polished specimens. The microhardness of both materials was also tested.
Fluorescent Carbon Dots for Super-Resolution Microscopy
Authors: Sun Xiangcheng , Mosleh Nazanin
Published: January 2023

Conventional fluorescence microscopy is limited by the optical diffraction of light, which results in a spatial resolution of about half of the light’s wavelength, approximately to 250–300 nm. The spatial resolution restricts the utilization of microscopes for studying subcellular structures. In order to improve the resolution and to shatter the diffraction limit, two general approaches were developed: a spatially patterned excitation method and a single-molecule localization strategy. The success of super-resolution imaging relies on bright and easily accessible fluorescent probes with special properties. Carbon dots, due to their unique properties, have been used for super-resolution imaging. Considering the importance and fast development of this field, this work focuses on the recent progress and applications of fluorescent carbon dots as probes for super-resolution imaging. The properties of carbon dots for super-resolution microscopy (SRM) are analyzed and discussed. The conclusions and outlook on this topic are also presented.
Polymer and Composite Materials in Two-Phase Passive Thermal Management Systems: A Review
Authors: Alqahtani Ali Ahmed , Bertola Volfango
Published: January 2023

The application of polymeric and composite materials in two-phase passive heat transfer devices is reviewed critically, with a focus on advantages and disadvantages of these materials in thermal management systems. Recent technology developments led to an increase of the power density in several applications including portable electronics, space and deployable systems, etc., which require high-performance and compact thermal management systems. In this context, passive two-phase systems are the most promising heat transfer devices to dissipate large heat fluxes without external power supply. Usually, heat transfer systems are built with metals due to their excellent thermal properties. However, there is an increasing interest in replacing metallic materials with polymers and composites that can offer cost-effectiveness, light weight and high mechanical flexibility. The present work reviews state-of the-art applications of polymers and composites in two-phase passive thermal management systems, with an analysis of their limitations and technical challenges.
Adding Value to Secondary Aluminum Casting Alloys: A Review on Trends and Achievements
Authors: Nunes Helder , Emadinia Omid , Soares Rui , Vieira Manuel F. , Reis Ana
Published: January 2023

Aluminum is a critical element of the circular economy as it can be recycled several times. Moreover, Al recycling is a more economically and environmentally efficient procedure than the primary Al production from ores. Secondary aluminum alloys are mostly used in casting applications since it is possible to accommodate their chemical composition through secondary manufacturing processes. However, the quality of the alloys may be considerably altered during the different steps of the recycling process. Inadequate waste sorting might result in excessive contamination. Iron is the most dangerous contaminant because it causes brittle and fragile intermetallic phases, which significantly impacts the mechanical characteristics of alloys. In addition, the microstructure of the alloy changes significantly after multiple cycles of remelting. These issues lead to the downcycling of aluminum, i.e., in other words, the reduction in the overall quality of the alloys. Thus, it has been shown that a number of procedures, including ultrasonic melt treatment and microalloying with rare earths, can somewhat alter the shape of the Fe-rich phases in order to reduce the shortcomings of downcycling. However, a solid mechanical characterization is still missing in order to improve the Fe-rich phase alteration.
Modeling and Vibration Control of Sandwich Composite Plates
Authors: Huang Zhicheng , Peng Huanyou , Wang Xingguo , Chu Fulei
Published: January 2023

A finite element dynamic model of the sandwich composite plate was developed based on classical laminate theory and Hamilton’s principle. A 4-node, 7-degree-of-freedom three-layer plate cell is constructed to simulate the interaction between the substrate, the viscoelastic damping layer, and the piezoelectric material layer. Among them, the viscoelastic layer is referred to as the complex constant shear modulus model, and the equivalent Rayleigh damping is introduced to represent the damping of the substrate. The established dynamics model has too many degrees of freedom, and the obtained dynamics model has good controllability and observability after adopting the joint reduced-order method of dynamic condensation in physical space and equilibrium in state space. The optimal quadratic (LQR) controller is designed for the active control of the sandwich panel, and the parameters of the controller parameters, the thickness of the viscoelastic layer, and the optimal covering position of the sandwich panel are optimized through simulation analysis. The results show that the finite element model established in this paper is still valid under different boundary conditions and different covering methods, and the model can still accurately and reliably represent the dynamic characteristics of the original system after using the joint step-down method. Under different excitation signals and different boundary conditions, the LQR control can effectively suppress the vibration of the sandwich plate. The optimal cover position of the sandwich plate is near the solid support end and far from the free-degree end. The parameters of controller parameters and viscoelastic layer thickness are optimized from several angles, respectively, and a reasonable optimization scheme can be selected according to the actual requirements.
Combining Ultrafiltration and Nanofiltration to Obtain a Concentrated Extract of Purified Polyphenols from Wet Olive Pomace
Authors: Sánchez-Arévalo Carmen M. , Pérez García-Serrano Ane , Vincent-Vela María Cinta , Álvarez-Blanco Silvia
Published: January 2023

Despite the environmental concerns raised every year by the generation of high volumes of wet olive pomace, it contains valuable phenolic compounds that are essential for the valorization of this by-product. In this work, an integrated process to recover phenolic compounds from wet olive pomace is proposed. It consists of ultrasound-assisted solid-liquid extraction, followed by ultrafiltration and nanofiltration. Several commercial membranes were studied at different operational conditions. The ultrafiltration stage allowed the purification of biophenols, which were obtained in the permeate stream. Regarding organic matter, satisfactory rejection values were obtained with both commercial UH030 and UP005 membranes (Microdyn Nadir), but the latter provided more efficient purification and higher values of permeate flux, above 18 L·h−1·m−2 at 2.5 bar and 1.5 m·s−1. Later, this permeate stream was concentrated by means of a nanofiltration process, obtaining polyphenol rejection values that surpassed 85% with the commercial NF270 membrane (DuPont), then achieving the concentration of the previously purified polyphenols.
Effect of CeO2 Content on Melting Performance and Microstructure of CaO-Al2O3-SiO2-MgO Refining Slag
Authors: Zhao Bo , Wu Wei , Zhi Jianguo , Su Cheng , Yao Tonglu
Published: January 2023

CeO2 can be applied to refining slag to minimize the size of inclusions, speed up the deoxidization process, and adsorb Al2O3 inclusions. The impact through which CeO2 content affects slag’s melting efficiency is still uncertain. The thermal analyzer was used to measure the thermogravimetric analysis (TG) and differential scanning calorimetry (DSC) curves of the slag melting process. According to the study results, with the increase in CeO2 content, the melting temperature of slag decreased first and then increased. The slag’s melting point fell from 1364 °C to 1324 °C and then rose to 1503 °C. XRD and SEM were used to analyze the CaO-Al2O3-SiO2-MgO-CeO2 slag’s microstructure. The mineral-phase structure of CeO2-containing refining slag was primarily composed of Ca2SiO4 and 3CaO·Al2O3, MgO, SiO2, CaO·Al2O3, and Ca8Ce6Al6O26. The proportion of 3CaO·Al2O3, CaO·Al2O3, and Ca2SiO4 decreased as the rare-earth-oxide content increased, while the proportion of Ca8Ce6Al6O26 increased. FactSage was used to estimate the equilibrium-phase compositions of slags with various compositions, and a model for predicting melting points was carried out by a linear regression model. Results were obtained through the analysis of equilibrium-phase composition and crystal structure transformation. The main reasons for the melting point decrease were the change of degree of polymerization and the decrease in contents and complete melting temperature of high-melting-point Ca3Al2O6 and Ca2SiO4 compounds. The latter increase in melting point was due to the number of Ca8Ce6Al6O26 compounds and precipitation temperature increases and the complexity of the structural-network increases.
Relation of Aortic Waveforms with Gut Hormones following Continuous and Interval Exercise among Older Adults with Prediabetes
Authors: Battillo Daniel J. , Malin Steven K.
Published: January 2023

Prediabetes raises cardiovascular disease risk, in part through elevated aortic waveforms. While insulin is a vasodilatory hormone, the gut hormone relation to aortic waveforms is less clear. We hypothesized that exercise, independent of intensity, would favor aortic waveforms in relation to gut hormones. Older adults (61.3 ± 1.5 yr; 33.2 ± 1.1 kg/m2) with prediabetes (ADA criteria) were randomized to undertake 60 min of work-matched continuous (CONT, n = 14) or interval (INT, n = 14) exercise for 2 wks. During a 180 min 75-g OGTT, a number of aortic waveforms (applanation tonometry) were assessed: the augmentation pressure (AP) and index (AIx75), brachial (bBP) and central blood pressure (cBP), pulse pressure (bPP and cPP), pulse pressure amplification (PPA), and forward (Pf) and backward pressure (Pb) waveforms. Acylated-ghrelin (AG), des-acylated ghrelin (dAG), GIP, and GLP-1active were measured, and correlations were co-varied for insulin. Independent of intensity, exercise increased VO2peak (p = 0.01) and PPA120min (p = 0.01) and reduced weight (p < 0.01), as well as AP120min (p = 0.02) and AIx75120min (p < 0.01). CONT lowered bSBP (p < 0.02) and bDBP (p < 0.02) tAUC180min more than INT. There were decreases dAG0min related to Pb120min (r = 0.47, p = 0.03), cPP120min (r = 0.48, p = 0.02), and AP120min (r = 0.46, p = 0.02). Declines in AG tAUC60min correlated with lower Pb120min (r = 0.47, p = 0.03) and cPP120min (r = 0.49, p = 0.02) were also found. GLP-1active 0min was reduced associated with lowered AP180min (r = 0.49, p = 0.02). Thus, while CONT exercise favored blood pressure, both intensities of exercise improved aortic waveforms in relation to gut hormones after controlling for insulin.
Strain Energy Dissipation Characteristics and Neural Network Model during Uniaxial Cyclic Loading and Unloading of Dry and Saturated Sandstone
Authors: Hao Yang , Wu Yu , Cui Ruoyu , Cao Kewang , Niu Dongdong , Liu Chunhui
Published: January 2023

The energy dissipation characteristics are important features of rock damage and failure during loading. However, the quantitative relationship between energy dissipation and rock failure is not clear. In this work, acoustic emission monitoring tests during uniaxial cyclic loading and unloading were conducted on sandstones in two conditions, namely dry and saturated, to investigate the energy evolution characteristics. Then, an index of the absolute energy ratio and a dynamic adjustment coefficient were put forward to represent rock damage and failure. A recurrent neural network was employed to predict the dynamic adjustment coefficient of dissipative strain energy. The results showed that (1) water saturation promoted the increased rate of dissipative strain energy during the last loading and unloading, but suppressed the sudden drop in elastic strain energy. (2) In the early and middle stages of cyclic loading–unloading, the absolute acoustic emission energy of dry and saturated rock samples was mainly generated by the historical maximum stress, while the absolute acoustic emission energy was mainly generated by cycle loading–unloading in the final cyclic stages. (3) The absolute energy ratio of both dry and saturated rock samples showed a sudden increase at the last cyclic loading–unloading, and this phenomenon can be taken as a precursor of rock damage of cycle loading–unloading. (4) The recurrent neural network for the prediction of the dynamic adjustment coefficient shows good agreement for rock failure related to dissipative strain energy. The results can provide experimental and prediction models for the monitoring and warning of rock engineering disasters in slopes, hydraulic fractures, tunnels, and coal mines.
Review on Extraction, Modification, and Synthesis of Natural Peptides and Their Beneficial Effects on Skin
Authors: An Jiabing , Tsopmejio Ivan Stève Nguepi , Wang Zi , Li Wei
Published: January 2023

Peptides, functional nutrients with a size between those of large proteins and small amino acids, are easily absorbed by the human body. Therefore, they are seeing increasing use in clinical medicine and have revealed immunomodulatory and anti-inflammatory properties which could make them effective in healing skin wounds. This review sorted and summarized the relevant literature about peptides during the past decade. Recent works on the extraction, modification and synthesis of peptides were reviewed. Importantly, the unique beneficial effects of peptides on the skin were extensively explored, providing ideas for the development and innovation of peptides and laying a knowledge foundation for the clinical application of peptides.
Improvement of the Chemical Reactivity of Michael Acceptor of Ethacrynic Acid Correlates with Antiproliferative Activities
Authors: El Abbouchi Abdelmoula , El Brahmi Nabil , Hiebel Marie-Aude , Ghammaz Hamza , El Fahime Elmostafa , Bignon Jérôme , Guillaumet Gérald , Suzenet Franck , El Kazzouli Saïd
Published: January 2023

The present study aims to report the design, synthesis, and biological activity of new ethacrynic acid (EA) analogs (6–10) obtained by the double modulation of the carboxylic acid moiety and the aromatic ring with the aim to increase the chemical reactivity of Michael acceptor of EA. All obtained compounds were characterized by 1H and 13C NMR, IR, and high-resolution mass spectrometry. The antiproliferative activity was evaluated in vitro using MMT test, in a first step, against HL60 cell line and in a second step, on a panel of human cancer cell lines such as HCT116, A549, MCF7, PC3, U87-MG, and SKOV3, and normal cell line MRC5 in comparison with positive control doxorubicin. Among all the tested compounds, the product 8 containing a propargyl and a hydroxyl groups, allowing an intramolecular hydrogen bond with the keto group of EA, exhibited a pronounced and selective activity in a nanomolar range against HL60, A549, PC3, and MCF7 with IC50 values of 15, 41.2, 68.7, and 61.5 nM, respectively. Compound 8 also showed a good selectivity index (SI) against HL60 and moderate SI against the other three human cancer cells (A549, PC3, and MCF7). The study of the structure-activity relationship showed that both modifications of the carboxylic group and the introduction of an intramolecular hydrogen bond are highly required to improve the antiproliferative activities. The molecular modeling studies of compound 8 revealed that it favorably binds to the glutathione S-transferase active site, which may explain its interesting anticancer activity. These new compounds have potential to be developed as novel therapeutic agents against various cancer types.
Toward Accurate yet Effective Computations of Rotational Spectroscopy Parameters for Biomolecule Building Blocks
Authors: Barone Vincenzo , Di Grande Silvia , Puzzarini Cristina
Published: January 2023

The interplay of high-resolution rotational spectroscopy and quantum-chemical computations plays an invaluable role in the investigation of biomolecule building blocks in the gas phase. However, quantum-chemical methods suffer from unfavorable scaling with the dimension of the system under consideration. While a complete characterization of flexible systems requires an elaborate multi-step strategy, in this work, we demonstrate that the accuracy obtained by quantum-chemical composite approaches in the prediction of rotational spectroscopy parameters can be approached by a model based on density functional theory. Glycine and serine are employed to demonstrate that, despite its limited cost, such a model is able to predict rotational constants with an accuracy of 0.3% or better, thus paving the way toward the accurate characterization of larger flexible building blocks of biomolecules.
Synthesis and Properties of Norphthalocyanines Functionalized with a Tetrathiacrown Ether–Tetrathiafulvalene Substituent
Authors: Hou Ruibin , Liu Xiaoyu , Xia Yan , Li Dongfeng
Published: January 2023

To construct novel ion receptors and D-A self-assembly systems for materials with better functions, the annulation of a tetrathiafulvalene donor with a magnesium norphthalocyanine core via a flexible tetrathiacrown ether bridge afforded a new triad 1. The structure of this product was characterized by 1H NMR and infrared spectroscopy, time-of-flight mass spectrometry, and elemental analysis. The optical and electrochemical properties were investigated using UV–vis spectroscopy and cyclic voltammetry. The complex of triad 1 and 2,3,5,6- tetrafluoro-7,7,8,8-tetracyanoquinodimethane produced electron transfer with a radical cationic character, as confirmed by UV–Vis and electron paramagnetic resonance analysis. Furthermore, the target compound presented evident intramolecular charge-transfer interactions in ground states, which were explained using density functional theory. Furthermore, norphthalocyanine 1 was able to coordinate Ag+ through the peripheral ligating oxathiaether crown.
Molecularly Imprinted Nanomaterials with Stimuli Responsiveness for Applications in Biomedicine
Authors: Zhang Yan , Wang Qinghe , Zhao Xiao , Ma Yue , Zhang Hongbo , Pan Guoqing
Published: January 2023

The review aims to summarize recent reports of stimuli-responsive nanomaterials based on molecularly imprinted polymers (MIPs) and discuss their applications in biomedicine. In the past few decades, MIPs have been proven to show widespread applications as new molecular recognition materials. The development of stimuli-responsive nanomaterials has successfully endowed MIPs with not only affinity properties comparable to those of natural antibodies but also the ability to respond to external stimuli (stimuli-responsive MIPs). In this review, we will discuss the synthesis of MIPs, the classification of stimuli-responsive MIP nanomaterials (MIP-NMs), their dynamic mechanisms, and their applications in biomedicine, including bioanalysis and diagnosis, biological imaging, drug delivery, disease intervention, and others. This review mainly focuses on studies of smart MIP-NMs with biomedical perspectives after 2015. We believe that this review will be helpful for the further exploration of stimuli-responsive MIP-NMs and contribute to expanding their practical applications especially in biomedicine in the near future.
Comparison of Antioxidant Capacity and Network Pharmacology of Phloretin and Phlorizin against Neuroinflammation in Traumatic Brain Injury
Authors: Ongay Kubra Kizil , Granato Daniel , Barreto George E.
Published: January 2023

Neuroinflammation is a hallmark of traumatic brain injury (TBI)’s acute and chronic phases. Despite the medical and scientific advances in recent years, there is still no effective treatment that mitigates the oxidative and inflammatory damage that affects neurons and glial cells. Therefore, searching for compounds with a broader spectrum of action that can regulate various inflammatory signaling pathways is of clinical interest. In this study, we determined not only the in vitro antioxidant capacity of apple pomace phenolics, namely, phlorizin and its metabolite, phloretin, but we also hypothesize that the use of these bioactive molecules may have potential use in TBI. We explored the antioxidant effects of both compounds in vitro (DPPH, iron-reducing capacity (IRC), and Folin–Ciocalteu reducing capacity (FCRC)), and using network pharmacology, we investigated the proteins involved in their protective effects in TBI. Our results showed that the antioxidant properties of phloretin were superior to those of phlorizin in the DPPH (12.95 vs. 3.52 mg ascorbic acid equivalent (AAE)/L), FCRC (86.73 vs. 73.69 mg gallic acid equivalent (GAE)/L), and iron-reducing capacity (1.15 vs. 0.88 mg GAE/L) assays. Next, we examined the molecular signature of both compounds and found 11 proteins in common to be regulated by them and involved in TBI. Meta-analysis and GO functional enrichment demonstrated their implication in matrix metalloproteinases, p53 signaling, and cell secretion/transport. Using MCODE and Pearson’s correlation analysis, a subcluster was generated. We identified ESR1 (estrogen receptor alpha) as a critical cellular hub being regulated by both compounds and with potential therapeutic use in TBI. In conclusion, our study suggests that because of their vast antioxidant effects, probably acting on estrogen receptors, phloretin and phlorizin may be repurposed for TBI treatment due to their ease of obtaining and low cost.
Classification and Multifaceted Potential of Secondary Metabolites Produced by Bacillus subtilis Group: A Comprehensive Review
Authors: Iqbal Sajid , Begum Farida , Rabaan Ali A. , Aljeldah Mohammed , Al Shammari Basim R. , Alawfi Abdulsalam , Alshengeti Amer , Sulaiman Tarek , Khan Alam
Published: January 2023

Despite their remarkable biosynthetic potential, Bacillus subtilis have been widely overlooked. However, their capability to withstand harsh conditions (extreme temperature, Ultraviolet (UV) and γ-radiation, and dehydration) and the promiscuous metabolites they synthesize have created increased commercial interest in them as a therapeutic agent, a food preservative, and a plant-pathogen control agent. Nevertheless, the commercial-scale availability of these metabolites is constrained due to challenges in their accessibility via synthesis and low fermentation yields. In the context of this rising in interest, we comprehensively visualized the antimicrobial peptides produced by B. subtilis and highlighted their prospective applications in various industries. Moreover, we proposed and classified these metabolites produced by the B. subtilis group based on their biosynthetic pathways and chemical structures. The biosynthetic pathway, bioactivity, and chemical structure are discussed in detail for each class. We believe that this review will spark a renewed interest in the often disregarded B. subtilis and its remarkable biosynthetic capabilities.