bluuebunny/crossref_metadata_2025 huggingface dataset:
A Survey of Pharmacogenomics Testing Among Physicians, Pharmacists, and Researchers From China
Authors: Guo Chengxian , Hu Biwen , Guo Chengjun , Meng Xiangguang , Kuang Yun , Huang Longjian , Wang Danling , Xu Kangwei , Zhao Yanlin , Yang Guoping , Cai Weimin , Shu Yan
Published: July 2021

To elucidate current domestic factors influencing pharmacogenomics (PGx) implementation and its future in China, we conducted a questionnaire survey on PGx applications and testing. A questionnaire-based survey was created on the popular online professional survey platform “Wenjuanxing” (www.wjx.cn) and performed via the social media platform WeChat. Among 422 participants, there were physicians (27.7%), pharmacists (31.3%), and researchers (41.0%). We found that less than 50% of physicians were aware of the importance of PGx in drug therapy, while over 50% of pharmacists and researchers recognized the importance. Only 38.5% of physicians, 40.9% of pharmacists, and 55.5% of researchers concurred that PGx analysis could lower the economic burdens for patients. However, most of the responders affirmed that PGx should be effectively implemented in clinical practices. A lack of sector standards, a lack of clinical research, and a lack of guidelines were found to be the major factors for hindering PGx clinical application. Among drugs associated with PGx assays, the most common were warfarin and clopidogrel. Although PGx research has advanced rapidly in recent years in mainland China, the clinical implementation of PGx has a long way to go.
Necroptosis: A Novel Pathway in Neuroinflammation
Authors: Yu Ziyu , Jiang Nan , Su Wenru , Zhuo Yehong
Published: July 2021

Neuroinflammation is a complex inflammatory process in the nervous system that is expected to play a significant role in neurological diseases. Necroptosis is a kind of necrosis that triggers innate immune responses by rupturing dead cells and releasing intracellular components; it can be caused by Toll-like receptor (TLR)-3 and TLR-4 agonists, tumor necrosis factor (TNF), certain microbial infections, and T cell receptors. Necroptosis signaling is modulated by receptor-interacting protein kinase (RIPK) 1 when the activity of caspase-8 becomes compromised. Activated death receptors (DRs) cause the activation of RIPK1 and the RIPK1 kinase activity-dependent formation of an RIPK1-RIPK3-mixed lineage kinase domain-like protein (MLKL), which is complex II. RIPK3 phosphorylates MLKL, ultimately leading to necrosis through plasma membrane disruption and cell lysis. Current studies suggest that necroptosis is associated with the pathogenesis of neuroinflammatory diseases, such as Alzheimer’s disease, Parkinson’s disease, and traumatic brain injury. Inhibitors of necroptosis, such as necrostatin-1 (Nec-1) and stable variant of Nec (Nec-1s), have been proven to be effective in many neurological diseases. The purpose of this article is to illuminate the mechanism underlying necroptosis and the important role that necroptosis plays in neuroinflammatory diseases. Overall, this article shows a potential therapeutic strategy in which targeting necroptotic factors may improve the pathological changes and clinical symptoms of neuroinflammatory disorders.
Cyclotides Isolated From Violet Plants of Cameroon Are Inhibitors of Human Prolyl Oligopeptidase
Authors: Gattringer Jasmin , Ndogo Olivier Eteme , Retzl Bernhard , Ebermann Carina , Gruber Christian W. , Hellinger Roland
Published: July 2021

Traditional medicine and the use of herbal remedies are well established in the African health care system. For instance, Violaceae plants are used for antimicrobial or anti-inflammatory applications in folk medicine. This study describes the phytochemical analysis and bioactivity screening of four species of the violettribeAllexis found in Cameroon.Allexis cauliflora,Allexis obanensis,Allexis batangaeandAllexis zygomorphawere evaluated for the expression of circular peptides (cyclotides) by mass spectrometry. The unique cyclic cystine-rich motif was identified in several peptides of all four species. Knowing that members of this peptide family are protease inhibitors, the plant extracts were evaluated for the inhibition of human prolyl oligopeptidase (POP). Since all four species inhibited POP activity, a bioactivity-guided fractionation approach was performed to isolate peptide inhibitors. These novel cyclotides, alca 1 and alca 2 exhibited IC50values of 8.5 and 4.4 µM, respectively. To obtain their amino acid sequence information, combinatorial enzymatic proteolysis was performed. The proteolytic fragments were evaluated in MS/MS fragmentation experiments and the full-length amino acid sequences were obtained byde novoannotation of fragment ions. In summary, this study identified inhibitors of the human protease POP, which is a drug target for inflammatory or neurodegenerative disorders.
Methadone, Buprenorphine, and Clonidine Attenuate Mitragynine Withdrawal in Rats
Authors: Hassan Rahimah , Sreenivasan Sasidharan , Müller Christian P. , Hassan Zurina
Published: July 2021

Background:Kratom orMitragyna speciosaKorth has been widely used to relieve the severity of opioid withdrawal in natural settings. However, several studies have reported that kratom may by itself cause dependence following chronic consumption. Yet, there is currently no formal treatment for kratom dependence. Mitragynine, is the major psychoactive alkaloid in kratom. Chronic mitragynine treatment can cause addiction-like symptoms in rodent models including withdrawal behaviour. In this study we assessed whether the prescription drugs, methadone, buprenorphine and clonidine, could mitigate mitragynine withdrawal effects. In order to assess treatment safety, we also evaluated hematological, biochemical and histopathological treatment effects.

Methods:We induced mitragynine withdrawal behaviour in a chronic treatment paradigm in rats. Methadone (1.0 mg/kg), buprenorphine (0.8 mg/kg) and clonidine (0.1 mg/kg) were i.p. administered over four days during mitragynine withdrawal. These treatments were stopped and withdrawal sign assessment continued. Thereafter, toxicological profiles of the treatments were evaluated in the blood and in organs.

Results:Chronic mitragynine treatment caused significant withdrawal behaviour lasting at least 5 days. Methadone, buprenorphine, as well as clonidine treatments significantly attenuated these withdrawal signs. No major effects on blood or organ toxicity were observed.

Conclusion:These data suggest that the already available prescription medications methadone, buprenorphine, and clonidine are capable to alleviate mitragynine withdrawal signs rats. This may suggest them as treatment options also for problematic mitragynine/kratom use in humans.
Species-Specific Effects of Cation Channel TRPM4 Small-Molecule Inhibitors
Authors: Arullampalam Prakash , Preti Barbara , Ross-Kaschitza Daniela , Lochner Martin , Rougier Jean-Sébastien , Abriel Hugues
Published: July 2021

Background: The Transient Receptor Potential Melastatin member 4 (TRPM4) gene encodes a calcium-activated non-selective cation channel expressed in several tissues. Mutations in TRPM4 have been reported in patients with different types of cardiac conduction defects. It is also linked to immune response and cancers, but the associated molecular mechanisms are still unclear. Thus far, 9-phenanthrol is the most common pharmacological compound used to investigate TRPM4 function. We recently identified two promising aryloxyacyl-anthranilic acid compounds (abbreviated CBA and NBA) inhibiting TRPM4. However, all aforementioned compounds were screened using assays expressing human TRPM4, whereas the efficacy of mouse TRPM4 has not been assessed. Mouse models are essential to investigate ion channel physiology and chemical compound efficacy.

Aim: In this study, we performed comparative electrophysiology experiments to assess the effect of these TRPM4 inhibitors on human and mouse TRPM4 channels heterologously expressed in TsA-201 cells.

Methods and Results: We identified striking species-dependent differences in TRPM4 responses. NBA inhibited both human and mouse TRPM4 currents when applied intracellularly and extracellularly using excised membrane patches. CBA inhibited human TRPM4, both intracellularly and extracellularly. Unexpectedly, the application of CBA had no inhibiting effect on mouse TRPM4 current when perfused on the extracellular side. Instead, its increased mouse TRPM4 current at negative holding potentials. In addition, CBA on the intracellular side altered the outward rectification component of the mouse TRPM4 current. Application of 9-phenanthrol, both intracellularly and extracellularly, inhibited human TRPM4. For mouse TRPM4, 9-phenanthrol perfusion led to opposite effects depending on the site of application. With intracellular 9-phenanthrol, we observed a tendency towards potentiation of mouse TRPM4 outward current at positive holding potentials.

Conclusion: Altogether, these results suggest that pharmacological compounds screened using “humanised assays” should be extensively characterised before application in vivo mouse models.
Sex Differences in Exercise-Training-Related Functional and Morphological Adaptation of Rat Gracilis Muscle Arterioles
Authors: Merkely Petra , Bakos Marcell , Bányai Bálint , Monori-Kiss Anna , Horváth Eszter M. , Bognár Judit , Benkő Rita , Oláh Attila , Radovits Tamás , Merkely Béla , Ács Nándor , Nádasy György L. , Török Marianna , Várbíró Szabolcs
Published: July 2021

Background

The cardiovascular effects of training have been widely investigated; however, few studies have addressed sex differences in arteriolar adaptation. In the current study, we examined the adaptation of the gracilis arterioles of male and female rats in response to intensive training.

Methods

Wistar rats were divided into four groups: male exercise (ME) and female exercise (FE) animals that underwent a 12-week intensive swim-training program (5 days/week, 200 min/day); and male control (MC) and female control (FC) animals that were placed in water for 5 min daily. Exercise-induced cardiac hypertrophy was confirmed by echocardiography. Following the training, the gracilis muscle arterioles were prepared, and their biomechanical properties and functional reactivity were tested, using pressure arteriography. Collagen and smooth muscle remodeling were observed in the histological sections.

Results

Left ventricular mass was elevated in both sexes in response to chronic training. In the gracilis arterioles, the inner radius and wall tension increased in female animals, and the wall thickness and elastic modulus were reduced in males. Myogenic tone was reduced in the ME group, whereas norepinephrine-induced vasoconstriction was elevated in the FE group. More pronounced collagen staining was observed in the ME group than in the MC group. Relative hypertrophy and tangential stress of the gracilis arterioles were higher in females than in males. The direct vasoconstriction induced by testosterone was lower in females and was reduced as an effect of exercise in males.

Conclusion

The gracilis muscle arteriole was remodeled as a result of swim training, and this adaptation was sex dependent.
Cecal Ligation and Puncture-Induced Sepsis Promotes Brown Adipose Tissue Inflammation Without Any Impact on Expression of Thermogenic-Related Genes
Authors: Moreno-Navarrete José María , Comas Ferran , de Jager Vincent , Fernández-Real José Manuel , Bouma Hjalmar R.
Published: July 2021

Background and Aims: The negative effects of chronic low-level inflammation on adipose tissue physiology have been extensively demonstrated, whereas the effects of acute inflammation are less studied. Here, we aimed to investigate the effects of sepsis-induced acute inflammation on gene expression markers of brown and white adipose tissue functionality.

Methods: Brown adipose tissue (BAT) and perirenal white adipose tissue (prWAT) gene expression markers were analyzed in cecal ligation and puncture (CLP)-induced sepsis mice model.

Results: CLP-induced sepsis attenuated expression of adipogenesis-related genes, in parallel to increased Tnf, Il6, and Ltf gene expression in prWAT. In contrast, CLP-induced sepsis resulted in increased expression of pro-inflammatory genes (Il6, Ltf, and Lbp) in BAT, without affecting expression of genes encoding for thermogenic activity.

Conclusion: Sepsis promotes both prWAT and BAT inflammation, associated with reduced adipogenesis-related gene expression in prWAT, without significant effects on BAT thermogenic genes.
Diversity in Surface Microstructures of Trichomes, Epidermal Cells, and Stomata in Lentil Germplasm
Authors: Patel Ishita , Gorim Linda Yuya , Tanino Karen , Vandenberg Albert
Published: July 2021

To develop crops capable of withstanding challenges posed by climate change, breeding strategies must focus on addressing multiple stresses occurring concurrently in plants. Leaf epidermal structures such as trichomes, stomata, and epidermal cells play an important role in mediating plant defense and could be essential traits that impart wide-ranging tolerance to biotic and abiotic stresses. Consequently, it is important to inform on the underlying diversity in these traits in lentil germplasm (Lens spp.). In this study, we characterized foliar microstructures of 12 genotypes belonging to seven wild and cultivated Lens species. We performed scanning electron microscopy on leaflet and pod surfaces for their qualitative characterization. For quantitative characterization, we observed surface imprints via light microscopy and quantified trichome density (TD), trichome length (TL), stomatal density (SD), epidermal cell density (ECD), and stomatal index (SI) on adaxial and abaxial leaflet surfaces for each genotype. We also assessed the heritability of trichome traits by evaluating interspecific recombinant inbred lines (RILs) derived from the cross Lens culinaris CDC Redberry × Lens tomentosus IG 72805. Comparing foliar microstructures, we found that TD and TL varied widely among cultivated and wild lentil genotypes. However, in most lentil genotypes, the adaxial leaflet surface had lower TD and longer trichomes compared to the abaxial surface. Pubescence on pods comprised five major phenotypes: no trichomes or glabrous pods, very short trichomes at low density, short trichomes at high density, medium-length trichomes at high density, and long trichomes at high density. Leaves of all species were amphistomatous, and SI, SD, and ECD were all higher on the adaxial compared to the abaxial surface. Adaxial surfaces had slightly sunken stomata, which might be an adaptive trait to conserve water. Quantifying TD and TL on the leaflets of interspecific RILs revealed transgressive segregation of these traits, suggesting that TD and TL are quantitative in nature. While taxonomic implications of this study are limited, a detailed description of agronomically relevant morphophysiological traits presented in this paper along with the mode of inheritance of trichomes may serve as a resource for scientists developing lentil adapted to concurrent biotic and abiotic stresses of the future.
Performing Multilingual Analysis With Linguistic Inquiry and Word Count 2015 (LIWC2015). An Equivalence Study of Four Languages
Authors: Dudău Diana Paula , Sava Florin Alin
Published: July 2021

Today, there is a range of computer-aided techniques to convert text into data. However, they convey not only strengths but also vulnerabilities compared to traditional content analysis. One of the challenges that have gained increasing attention is performing automatic language analysis to make sound inferences in a multilingual assessment setting. The current study is the first to test the equivalence of multiple versions of one of the most appealing and widely used lexicon-based tools worldwide, Linguistic Inquiry and Word Count 2015 (LIWC2015). For this purpose, we employed supervised learning in a classification problem and computed Pearson's correlations and intraclass correlation coefficients on a large corpus of parallel texts in English, Dutch, Brazilian Portuguese, and Romanian. Our findings suggested that LIWC2015 is a valuable tool for multilingual analysis, but within-language standardization is needed when the aim is to analyze texts sourced from different languages.
Cultural Differences in People’s Psychological Response to COVID-19
Authors: Yap Suhui , Lee Albert , Ji Li-Jun , Li Ye , Dong Ying
Published: July 2021

The present research studied Chinese and Euro-Canadian students during the COVID-19 pandemic, focusing on their affect, optimism, well-being, and meaning in life. The results revealed both differences and similarities across cultures. As predicted, Chinese participants reported more positive affect and less negative affect, higher optimism, higher state psychological well-being, and higher meaning presence, compared to Euro-Canadian participants. The findings were replicated after a week’s delay. Analyses on longitudinal data showed that state optimism, state well-being, and meaning presence influenced one another over time. These variables also mediated the cultural differences in one another. These results are consistent with cultural work on naïve dialecticism and non-linear lay theory of change. Results also demonstrate underlying relationships among the constructs that are common to both cultural groups. Broadly, the present research highlights the impact of culture on people’s response to challenging life situations and the mechanisms underlying these cultural differences.
What Type of Body Shape Moves Children? An Experimental Exploration of the Impact of Narrative Cartoon Character Body Shape on Children’s Narrative Engagement, Wishful Identification, and Exercise Motivation
Authors: Alon Dar , Sousa Caio Victor , Lu Amy Shirong
Published: July 2021

Background

The incorporation of narratives helps to enhance children’s engagement in active video games (AVGs), thus increasing moderate-to-vigorous physical activity (MVPA). Specific narrative elements, such as the visual representation of the characters’ body shape, have been rarely manipulated to explore their role in modulating children’s narrative engagement (NE) and exercise motivation.

Objective

To investigate the effects of character body shape manipulation (overweight/obese, average, or athletic slim) on children’s narrative immersion (NI), NE, wishful identification (WI), as well as their mediating effect on AVG and PA motivation.

Methods

Children ages 8–12 years old (N = 87) were randomly assigned to watch a 15-min animated video (designed for an existing AVG) in which the main characters had an overweight/obese, or average∗, or athletic slim body shape (all other elements were identical). Children’s NI, NE, WI, and AVG and physical activity (PA) motivation were then assessed.

Results

Controlling for social desirability, the analysis indicated that participants with a BMI of greater than the 75th percentile had a significantly higher NI, NE, WI, and PA motivation when video characters were set to the overweight/obese condition, than they did for video characters set to the average or athletic slim conditions. On the other hand, children of equal or less than the 75th percentile exposed to the average character body condition had a greater NE, WI, and PA motivation than overweight/obese or athletic slim conditions. A mediation analysis with structural equation modeling indicated that NE mediated the effect between character body shape and AVG and PA motivation.

Conclusion

Narrative cartoon characters that mirror the target participant’s body shape can increase NE, which in turn mediates AVG and PA motivation. Content producers should identify optimal strategies in character body shape design to encourage children of different weight status to participate in PA with engaging stories to maximize health narratives’ persuasive potentials.

∗The term average in this sense is not in reference to the national average body weight, but rather an average of the body weights represented in conditions A and C.
Transcultural Adaptation and Psychometric Proprieties of the Mental Toughness Inventory for Brazilian Athletes
Authors: Moreira Caio Rosas , Codonhato Renan , Fiorese Lenamar
Published: July 2021

This study has assessed the psychometric proprieties of the Mental Toughness Inventory (MTI) within the context of Brazilian sports. About 12 professionals participated in the process of adapting and translating the scale to Brazilian Portuguese. Subjects were 575 athletes (23.54 ± 5.79 years old; 58% males) who answered the MTI and the 10-item Connor–Davidson Resilience Scale (CD-RISC-10). Data were analyzed through confirmatory factor analysis (CFA), Cronbach's alpha (α), composite reliability (CR), average variance extracted (AVE), Spearman correlation, and model invariance tests. Results from CFA showed adequate fit for the original 8-item structure of the scale [Chi-square (χ2) = 27.041; p = 0.078; normalized chi-square (χ2/df) = 1.50; comparative fit index (CFI) = 0.988; Tucker–Lewis Index (TLI) = 0.981; root mean square error of approximation (RMSEA) = 0.03 [0.00–0.05]; standardized root mean residual (SRMR) = 0.030] assessing mental toughness (MT) as a single factor and the scale presented satisfactory internal consistency (CR = 0.81; α = 0.82). MT was correlated with resilience (r = 0.607), age (r = 0.276), and time of experience in the sport (r = 0.215). The MTI has also shown partial measurement invariance for sex and complete invariance across sport types. It was concluded that the MTI is a suitable tool for assessing MT in the present sample of Brazilian athletes; this instrument has potential practical application for researchers and sports psychologists who seek to develop the well-being and performance of athletes.
Bilateral Interference in Motor Performance in Homologous vs. Non-homologous Proximal and Distal Effectors
Authors: Aune Morten Andreas , Lorås Håvard , Nynes Alexander , Aune Tore Kristian
Published: July 2021

Performance of bimanual motor actions requires coordinated and integrated bilateral communication, but in some bimanual tasks, neural interactions and crosstalk might cause bilateral interference. The level of interference probably depends on the proportions of bilateral interneurons connecting homologous areas of the motor cortex in the two hemispheres. The neuromuscular system for proximal muscles has a higher number of bilateral interneurons connecting homologous areas of the motor cortex compared to distal muscles. Based on the differences in neurophysiological organization for proximal vs. distal effectors in the upper extremities, the purpose of the present experiment was to evaluate how the level of bilateral interference depends on whether the bilateral interference task is performed with homologous or non-homologous effectors as the primary task. Fourteen participants first performed a unilateral primary motor task with the dominant arm with (1) proximal and (2) distal controlled joysticks. Performance in the unilateral condition with the dominant arm was compared to the same effector’s performance when two different bilateral interference tasks were performed simultaneously with the non-dominant arm. The two different bilateral interference tasks were subdivided into (1) homologous and (2) non-homologous effectors. The results showed a significant decrease in performance for both proximal and distal controlled joysticks, and this effect was independent of whether the bilateral interference tasks were introduced with homologous or non-homologous effectors. The overall performance decrease as a result of bilateral interference was larger for proximal compared to distal controlled joysticks. Furthermore, a proximal bilateral interference caused a larger performance decrement independent of whether the primary motor task was controlled by a proximal or distal joystick. A novel finding was that the distal joystick performance equally interfered with either homologous (distal bilateral interference) or non-homologous (proximal bilateral interference) interference tasks performed simultaneously. The results indicate that the proximal–distal distinction is an important organismic constraint on motor control and for understanding bilateral communication and interference in general and, in particular, how bilateral interference caused by homologous vs. non-homologous effectors impacts motor performance for proximal and distal effectors. The results seem to map neuroanatomical and neurophysiological differences for these effectors.
Susceptibility to Scams in Older Black and White Adults
Authors: Han S. Duke , Barnes Lisa L. , Leurgans Sue , Yu Lei , Stewart Christopher C. , Lamar Melissa , Glover Crystal M. , Bennett David A. , Boyle Patricia A.
Published: July 2021

Previous reports on racial differences in scam susceptibility have yielded mixed findings, and few studies have examined reasons for any observed race differences. Older Black and White participants without dementia (N = 592) from the Minority Aging Research Study and the Rush Memory and Aging Project who completed a susceptibility to scam questionnaire and other measures were matched according to age, education, sex, and global cognition using Mahalanobis distance. In adjusted models, older Black adults were less susceptible to scams than older White adults (Beta = −0.2496, SE = 0.0649, p = 0.0001). Contextual factors did not mediate and affective factors did not moderate this association. Analyses of specific items revealed Black adults had greater knowledge of scam targeting of older adults and were less likely to pick up the phone for unidentified callers. Older Black adults are less susceptible to scams than demographically-matched older White adults, although the reasons remain unknown.
The Prevalence of Post-traumatic Stress Disorder Symptoms, Sleep Problems, and Psychological Distress Among COVID-19 Frontline Healthcare Workers in Taiwan
Authors: Lu Mei-Yun , Ahorsu Daniel Kwasi , Kukreti Shikha , Strong Carol , Lin Yi-Hsuan , Kuo Yi-Jie , Chen Yu-Pin , Lin Chung-Ying , Chen Po-Lin , Ko Nai-Ying , Ko Wen-Chien
Published: July 2021

The adverse effect of COVID-19 pandemic among individuals has been very disturbing especially among healthcare workers. This study aims to examine the prevalence of post-traumatic stress disorder (PTSD) symptoms, sleep problems, and psychological distress among COVID-19 frontline healthcare workers in Taiwan. Hence, a total of 500 frontline healthcare workers were recruited to participate in this cross-sectional study. They responded to measures on fear of COVID-19, depression, anxiety, stress, insomnia, PTSD, perceived stigma, and self-stigma. The results indicated a prevalence rate of 15.4% for PTSD symptoms, 44.6% for insomnia, 25.6% for depressive symptoms, 30.6% for anxiety symptoms, and 23.4% for stress among the participants. There were significantly positive interrelationships between all these variables. Anxiety symptoms and fear of COVID-19 predicted PTSD whereas symptoms of anxiety, fear of COVID-19, and stress predicted insomnia. The prevalence rates of the psychological problems reveal a worrying view of mental health challenges among Taiwanese frontline healthcare workers. Anxiety symptoms and fear of COVID-19 are the common predictive factors of PTSD and sleep problems suggesting that mental healthcare services for them may help prevent future occurrence of psychological problems by allaying fears of healthcare workers. Therefore, there should be mental healthcare services for healthcare workers during the COVID-19 pandemic.
COVID-19 Pandemic as an Excellent Opportunity for Global Health Diplomacy
Authors: Taghizade Sanaz , Chattu Vijay Kumar , Jaafaripooyan Ebrahim , Kevany Sebastian
Published: July 2021

Undoubtedly, the COVID-19 pandemic is not the first and most frightening global pandemic, and it may not be the last. At the very least, this phenomenon has though seriously challenged the health systems of the world; it has created a new perspective on the value of national, regional, and international cooperation during crises. The post-coronavirus world could be a world of intensified nationalist rivalries on the economic revival and political influence. However, strengthening cooperation among nations at different levels will lead to the growth of health, economy, and security. The current situation is a touchstone for international actors in coordinating the efforts in similar future crises. At present, this pandemic crisis cannot be resolved except through joint international cooperation, global cohesion, and multilateralism. This perspective concludes that the pandemic could be an excellent opportunity for the scope of global health diplomacy (GHD) and how it can be applied and practiced for strengthening five global arenas, namely (1) International Cooperation and Global Solidarity, (2) Global Economy, Trade and Development, (3) Global Health Security, (4) Strengthening health systems, and (5) Addressing inequities to achieve the global health targets. GHD proves to be very useful for negotiating better policies, stronger partnerships, and achieving international cooperation in this phase with many geopolitical shifts and nationalist mindset among many nations at this stage of COVID-19 vaccine roll-out.
Forms of Health System Fragmentation During Conflict: The Case of Yemen
Authors: Dureab Fekri , Hussain Taha , Sheikh Rashad , Al-Dheeb Najwa , Al-Awlaqi Sameh , Jahn Albrecht
Published: July 2021

The continuous and protracted conflict in Yemen has evolved into the worst humanitarian situation in modern history. All public structures in the country, including the health system and its basic functions, have been under tremendous pressures. One of the key obstacles to improve the health outcomes in Yemen is fragmentation of the health system. This study aims at exploring and documenting the forms of health system fragmentation in humanitarian and conflict-affected contexts by studying Yemen as a case study. We collected national qualitative data from key informants through in-depth interviews. A pool of respondents was identified from the Ministry of Public Health and Population, donors, and non-governmental organizations. Data were collected between May and June 2019. We interviewed eight key informants and reviewed national health policy documents, and references provided by key informants. Interviews were recorded, transcribed, and analyzed using qualitative content analysis. We further conducted a literature review to augment and triangulate the findings. Six themes emerged from our datasets and analyses, representing various forms of fragmentation: political, structural, inter-sectoral, financial, governance, and health agenda-related forms. Health system fragmentation in Yemen existed before the conflict eruption and has aggravated as the conflict evolves. The humanitarian situation and the collapsing health system enabled the influx of various national and international health actors. In conclusion, the protracted conflict and fragile situation in Yemen have accentuated the fragmentation of the health system. Addressing these fragmentations' forms by all health actors and building consensus on health system agenda are recommended. Health system analysis and in-depth study of fragmentation drivers in Yemen can be beneficial to build common ground and priorities to reduce health system fragmentation. Furthermore, capacity building of a health system is fundamental for the humanitarian development nexus, health system integration, and recovery efforts in the future.
Optimizing Healthcare Through Digital Health and Wellness Solutions to Meet the Needs of Patients With Chronic Disease During the COVID-19 Era
Authors: Seixas Azizi A. , Olaye Iredia M. , Wall Stephen P. , Dunn Pat
Published: July 2021

The COVID-19 pandemic exposed and exacerbated longstanding inefficiencies and deficiencies in chronic disease management and treatment in the United States, such as a fragmented healthcare experience and system, narrowly focused services, limited resources beyond office visits, expensive yet low quality care, and poor access to comprehensive prevention and non-pharmacological resources. It is feared that the addition of COVID-19 survivors to the pool of chronic disease patients will burden an already precarious healthcare system struggling to meet the needs of chronic disease patients. Digital health and telemedicine solutions, which exploded during the pandemic, may address many inefficiencies and deficiencies in chronic disease management, such as increasing access to care. However, these solutions are not panaceas as they are replete with several limitations, such as low uptake, poor engagement, and low long-term use. To fully optimize digital health and telemedicine solutions, we argue for the gamification of digital health and telemedicine solutions through a pantheoretical framework—one that uses personalized, contextualized, and behavioral science algorithms, data, evidence, and theories to ground treatments.
Seasonal Patterns in Incidence and Antimicrobial Resistance of Common Bacterial Pathogens in Nursing Home Patients and Their Rooms
Authors: Cassone Marco , Mantey Julia , Gontjes Kyle J. , Lansing Bonnie J. , Gibson Kristen E. , Wang Joyce , Mody Lona
Published: July 2021

Background: Colonization is the main precursor to infection, which may lead to adverse clinical outcomes among older adults in nursing homes (NHs). Understanding seasonal changes in the local burden of common bacterial pathogens is key to implementing appropriate and cost-effective infection prevention measures in this resource-constrained healthcare environment. It is thus surprising that seasonal trends in patient and environmental colonization with major bacterial pathogens are presently unknown in the expanding NH setting.

Methods: We examined the seasonal incidence of four major pathogens among 640 nursing home patients and high-touch surfaces within their rooms over 2 years. In cases where a significant number of antimicrobial-resistant strains was found, incidence in antimicrobial-susceptible and antimicrobial-resistant isolates was compared, along with antibiotic use trends.

Results: We observed spring peaks in the incidence of vancomycin-resistant enterococci (1.70 peak to trough ratio for both patient and environmental isolates) and methicillin-resistant Staphylococcus aureus (1.95 peak to trough ratio for patient isolates, 1.50 for environmental isolates). We also observed summer peaks in Klebsiella pneumoniae (1.83 and 1.82 peak to trough ratio for patient and environmental isolates, respectively), and ciprofloxacin-resistant Escherichia coli. Susceptible S. aureus and E. coli did not follow seasonal patterns.

Conclusions: A meaningful seasonal pattern may be present in the NH setting for several significant pathogens, and especially antimicrobial-resistant ones. Whether such patterns are consistent across geographic areas and over longer periods of time should be a key focus of investigation, in order to better inform timing of surveillance and infection prevention efforts in this setting.
Practical Solutions to Address COVID-19-Related Mental and Physical Health Challenges Among Low-Income Older Adults
Authors: Bergeron Caroline D. , Boolani Ali , Jansen Erica C. , Smith Matthew Lee
Published: July 2021

Low-income older adults are disproportionately impacted by the COVID-19 pandemic. In this perspective article, we review the context in which low-income older people experience the pandemic and the mental and physical health consequences they have faced to date. Then, we offer practical solutions to help improve low-income older adults' sleep, physical activity, nutrition, and stress that require no or low financial commitment. We argue that governments, communities, and organizations should make greater efforts to promote healthy living for low-income older adults in times of health emergencies to ensure their ability to be universally adopted, regardless of income and resources.
A New Strategy to Quantitatively Identify Hot-Spot Areas in Growth of New HIV Infections for Targeted Interventions
Authors: Zhu Qiyu , JiKe Chunnong , Xu Chengdong , Liang Shu , Yu Gang , Wang Ju , Xiao Lin , Liu Ping , Chen Meibin , Guan Peng , Liu Zhongfu , Jin Cong
Published: July 2021

Background: Previous geographic studies of HIV infection have usually used prevalence data, which cannot indicate the hot-spot areas of current transmission. To develop quantitative analytic measures for accurately identifying hot-spot areas in growth of new HIV infection, we investigated the geographic distribution features of recent HIV infection and long-term HIV infection using data from a whole-population physical examination in four key counties in Liangshan prefecture, which are most severely affected by HIV in China.

Methods: Through a whole-population physical examination during November 2017- June 2018 in the four key counties, a total of 5,555 HIV cases were diagnosed and 246 cases were classified as recently infected by laboratory HIV recency tests. The geospatial patterns of recent and long-term HIV infected cases were compared using ordinary least squares regression and Geodetector. Further, geospatial-heterogeneity was quantified and indicated using a residual map to visualize hot-spot areas where new infection is increasing.

Results: The geographic location of HIV cases showed an uneven distribution along major roads and clustered at road intersections. The geographic mapping showed that several areas were clustered with more recently infected HIV cases than long-term infected cases. The quantitative analyses showed that the geospatial asymmetry between recent and long-term HIV infection was 0.30 and 0.31 in ordinary least squares regression and Geodetector analysis, respectively. The quantitative analyses found twenty-three townships showing an increase in the number of recent infections.

Conclusions: Quantitative analysis of geospatial-heterogeneous areas by comparing between recent and long-term HIV infections allows accurate identification of hot-spot areas where new infections are expanding, which can be used as a potent methodological tool to guide targeted interventions and curb the spread of the epidemic.
Cost-Effectiveness Analysis of High-Risk Groups Tuberculosis Screening in Malaysia
Authors: Mohd Hassan Nor Zam Azihan , Razali Asmah , Shahari Mohd Ridzwan , Mohd Nor Sham Kunusagaran Mohd Shaiful Jefri , Halili Juanita , Zaimi Nur Amalina , Bahari Mohd Shahri , Aminuddin Farhana
Published: July 2021

Screening of high-risk groups for Tuberculosis (TB) is considered as the cornerstone for TB elimination but the measure of cost-effectiveness is also crucial in deciding the strategy for TB screening. This study aims to measure the cost-effectiveness of TB screening between the various high-risk groups in Malaysia. A decision tree model was developed to assess the cost-effectiveness of TB screening among the high-risk groups from a provider perspective using secondary data from the year 2016 to 2018. The results are presented in terms of an Incremental Cost-Effectiveness Ratio (ICER), expressed as cost per TB case detected. Deterministic and Probabilistic Sensitivity Analysis was also performed to measure the robustness of the model. TB screening among Person Living with Human Immunodeficiency Virus (PL HIV) was the most cost-effective strategy, with MYR 2,597.00 per TB case detected. This was followed by elderly, prisoners and smokers with MYR 2,868.62, MYR 3,065.24, and MYR 4,327.76 per one TB case detected, respectively. There was an incremental cost of MYR 2.49 per screening, and 3.4 TB case detection per 1,000 screening for TB screening among PL HIV in relation to TB screening among prisoners. The probability of symptomatic cases diagnosed as TB was the key driver for increasing cost-effectiveness efficacy among PL HIV. Results of the study suggest prioritization of high-risk group TB screening program by focusing on the most cost-effective strategy such as screening among PL HIV, prisoners and elderly, which has a lower cost per TB case detected.
Discovering and Summarizing Relationships Between Chemicals, Genes, Proteins, and Diseases in PubChem
Authors: Zaslavsky Leonid , Cheng Tiejun , Gindulyte Asta , He Siqian , Kim Sunghwan , Li Qingliang , Thiessen Paul , Yu Bo , Bolton Evan E.
Published: July 2021

The literature knowledge panels developed and implemented in PubChem are described. These help to uncover and summarize important relationships between chemicals, genes, proteins, and diseases by analyzing co-occurrences of terms in biomedical literature abstracts. Named entities in PubMed records are matched with chemical names in PubChem, disease names in Medical Subject Headings (MeSH), and gene/protein names in popular gene/protein information resources, and the most closely related entities are identified using statistical analysis and relevance-based sampling. Knowledge panels for the co-occurrence of chemical, disease, and gene/protein entities are included in PubChem Compound, Protein, and Gene pages, summarizing these in a compact form. Statistical methods for removing redundancy and estimating relevance scores are discussed, along with benefits and pitfalls of relying on automated (i.e., not human-curated) methods operating on data from multiple heterogeneous sources.
Global Agri-Food Sector: Challenges and Opportunities in COVID-19 Pandemic
Authors: Hamid Saima , Mir Mohammad Yaseen
Published: July 2021

COVID-19 pandemic has been catastrophic for almost everything including the global economy. Among many sectors, the food and the agriculture sector was the worst hit following the immediate lockdown and market shutdowns. Though some stability was prevalent from supply side till date, however, the severe restrictions put in place to curb the spread of pandemic have endangered the supply of agricultural and food articles contemporaneously across borders and from field to fork. While the income decline due to price falland supplies chain disruptions due to pandemic have escalated the food shortages in several of developing and developed countries. Nevertheless the global demand for food items has remained more or less unchanged owing to their inelastic demand. Even within the global level, the scenario of food security and supply chain stability has been substantially deplorable for emerging and less developing countries due to their lack of insulation to the global shocks or pandemics. Notably, the technological backwardness, excessive know-how dependence and denied accessibility on several grounds lead to poverty and food hunger in these countries. At the policy level, a holistic approach specifically targeted towards the developing and less developed economies is highly warranted to ensure an appreciable progress towards the minimisation of sensitivity with regard to agriculture and food security. Apart from the measures to insulate them from global shocks, additional steps need to be taken to alleviate their technological backwardness and denied accessibility on certain socio-cultural norms.
ChipSats for Planetary Exploration: Dynamics and Aerothermal Modeling of Atmospheric Entry and Dispersion
Authors: Umansky-Castro Joshua S. , Yap Kimberly G. , Peck Mason A.
Published: July 2021

This paper presents an orbit-to-ground model for the atmospheric entry of ChipSats, gram-scale spacecraft that offer unique advantages over their conventionally larger counterparts. ChipSats may prove particularly useful for in-situ measurements in the upper atmosphere, where spatially and temporally varying phenomena are especially difficult to characterize. Globally distributed ChipSats would enable datasets of unprecedented detail, assuming they could survive. The model presented is used to assess the survival and dispersion of a swarm of ChipSats when deployed over the Earth, Moon, Mars, and Titan. These planetary exploration case studies focus on the Monarch, the newest-generation ChipSat developed at Cornell University, in order to evaluate technology readiness for such missions. A parametric study is then conducted to inform future ChipSat design, highlighting the role of the ballistic coefficient in both peak entry temperature and mission duration.
Viral Metagenomics Reveals Diverse Viruses in the Feces Samples of Raccoon Dogs
Authors: Yang Shixing , He Yumin , Chen Xu , Kalim Ullah , Wang Yan , Yang Shuyu , Qi Haifeng , Cheng HengZheng , Lu Xiang , Wang Xiaochun , Shen Quan , Zhang Wen
Published: July 2021

Raccoon dogs as an ancient species of Canidae are the host of many viruses, including rabies virus, canine distemper virus, severe acute respiratory syndrome coronavirus, and so on. With the development of raccoon dog breeding in recent years, some viruses which infected poultry or pigs were also detected from raccoon dogs. At present, the fecal virome of raccoon dogs has been rarely studied. Using an unbiased viral metagenomic approach, we investigated the fecal virome in raccoon dogs collected from one farm of Jilin Province, China. Many DNA or RNA viruses identified in those fecal samples were mainly from seven families, including Circoviridae, Smacoviridae, Genomoviridae, Parvoviridae, Picornaviridae, Astroviridae, and Hepeviridae. This study increased our understanding of the fecal virome in raccoon dog and provided valuable information for the monitoring, prevention, and treatment of viral diseases of these animals.
Tomato Leaf Disease Diagnosis Based on Improved Convolution Neural Network by Attention Module
Authors: Zhao Shengyi , Peng Yun , Liu Jizhan , Wu Shuo
Published: July 2021

Crop disease diagnosis is of great significance to crop yield and agricultural production. Deep learning methods have become the main research direction to solve the diagnosis of crop diseases. This paper proposed a deep convolutional neural network that integrates an attention mechanism, which can better adapt to the diagnosis of a variety of tomato leaf diseases. The network structure mainly includes residual blocks and attention extraction modules. The model can accurately extract complex features of various diseases. Extensive comparative experiment results show that the proposed model achieves the average identification accuracy of 96.81% on the tomato leaf diseases dataset. It proves that the model has significant advantages in terms of network complexity and real-time performance compared with other models. Moreover, through the model comparison experiment on the grape leaf diseases public dataset, the proposed model also achieves better results, and the average identification accuracy of 99.24%. It is certified that add the attention module can more accurately extract the complex features of a variety of diseases and has fewer parameters. The proposed model provides a high-performance solution for crop diagnosis under the real agricultural environment.
Effects of Cover Crop and Tillage Method Combinations on the Microbiological Traits of Spring Wheat (Triticum aestivum L.)
Authors: Majchrzak Leszek , Bocianowski Jan , Niewiadomska Alicja
Published: July 2021

We carried out multivariate characterisation of phenotypic variability in 27 treatments that were combinations of cover crop, tillage method, and year. Canonical variate analysis was employed to observe nine microbiological traits in an experiment established in a split-plot design. Between 2011–2013, a field experiment was conducted on soils classified as Albic Luvisols. The first-order factor was analysis of the effect of sowing a stubble cover crop: (Control: No cover crop sown; cover crop sown after skimming; no tillage: Cover crop sown directly). The second experimental factor involved evaluating the effects of three tillage methods (direct sowing; simplified tillage to a depth of 12–15 cm with a combined cultivator; spring ploughing to a depth of 25 cm) on nine microbiological traits. The year of research was used as a factor to differentiate between the count and activity of soil microorganisms. The traits (moulds and copiotrophic bacteria, and oligotrophic bacteria and actinobacteria) were significantly correlated (positively or negatively) at all five terms. Pearson’s test confirmed the relationships between the physiological groups of soil microorganisms after the application of organic matter, and captured the positive correlation between moulds and copiotrophs at all terms of the study.