bluuebunny/crossref_metadata_2025 huggingface dataset:
Humidification–Cooling System in Semi-Insulated Box-Type Cowsheds Prevent the Loss of Milk Productivity Due to Thermal Stress
Authors: Bleizgys Rolandas , Naujokienė Vilma , Čėsna Jonas
Published: May 2022

Heat stress is becoming an increasingly important problem in Lithuanian cowsheds. Microclimate formation systems were evaluated in the seven most popular cowsheds in Lithuania, with different wall and roof constructions, insulation, ventilation intensity controls, and one cowshed was additionally equipped with an air-cooling system—the air is cooled by spraying water droplets with a high pressure. In cowsheds equipped with fans to intensify the movement of air, the temperature does not fall below the outdoor temperature and the temperature humidity index (THI) is higher than outdoors. During the heat period, the THI rises to 82 and the cows get moderate thermal stress, which adversely affects feed intake and milk yield of dairy cows. In the cowshed, where the air humidification–cooling system is installed, the air temperature during heat is lower than the average in the field of 2.61 ± 0.74 °C. Although the relative humidity in the cowshed is on average 16.29 ± 4.12% wetter during heat than outside, the THI in the barn is lower than outside. The air temperature in this cowshed decreases by 3.17 ± 0.86 °C compared to cowsheds without an air humidification–cooling system. The air humidification–cooling system creates good conditions to reduce the conditions for cows to experience thermal stress. Further research is needed to optimize the amount of water spray and droplet size and increase the efficiency of the cooling system.
Nitrogen Critical Level in Leaves in ‘Chardonnay’ and ‘Pinot Noir’ Grapevines to Adequate Yield and Quality Must
Authors: Tassinari Adriele , Stefanello Lincon Oliveira , Schwalbert Rai Augusto , Vitto Beatriz Baticini , Kulmann Matheus Severo de Souza , Santos João Pedro Jung , Arruda Wagner Squizani , Schwalbert Raissa , Tiecher Tadeu Luis , Ceretta Carlos Alberto , De Conti Lessandro , Schumacher Rafael Lizandro , Brunetto Gustavo
Published: May 2022

The nitrogen (N) critical level in leaves and maximum technical efficiency (MTE) doses contribute to the grape yield, must quality, and fertilizers rationalization. This study aimed to define sufficient ranges and critical levels in leaves and maximum technical efficiency doses to obtain high yields and quality must in grapevines grown in sandy soil in a subtropical climate. ‘Chardonnay’ and ‘Pinot Noir’ grapevines were subjected to the annual application of 0, 10, 20, 40, 60, and 80 kg N ha−1 in a vineyard. The nitrogen concentration in leaves at flowering and veraison, grape yield and grape must chemical parameters (total soluble solids—TSS, total titratable acidity—TTA and total anthocyanins—TA) were evaluated. The N critical level in leaves at flowering was different between grapevines cultivars, but this was not observed in leaves at veraison. It was possible to estimate MTE for ‘Chardonnay’ grapevines, in the evaluated growing seasons. In the range of higher probability of the N critical level in leaves, the TSS and TTA variables showed opposite responses, for both cultivars. This study proposes different N critical levels for red and white grapevines, in a subtropical climate.
Application of Artificial Neural Networks Sensitivity Analysis for the Pre-Identification of Highly Significant Factors Influencing the Yield and Digestibility of Grassland Sward in the Climatic Conditions of Central Poland
Authors: Niedbała Gniewko , Wróbel Barbara , Piekutowska Magdalena , Zielewicz Waldemar , Paszkiewicz-Jasińska Anna , Wojciechowski Tomasz , Niazian Mohsen
Published: May 2022

Progressive climate changes are the most important challenges for modern agriculture. Permanent grassland represents around 70% of all agricultural land. In comparison with other agroecosystems, grasslands are more sensitive to climate change. The aim of this study was to create deterministic models based on artificial neural networks to identify highly significant factors influencing the yield and digestibility of grassland sward in the climatic conditions of central Poland. The models were based on data from a grassland experiment conducted between 2014 and 2016. Phytophenological data (harvest date and botanical composition of sward) and meteorological data (average temperatures, total rainfall, and total effective temperatures) were used as independent variables, whereas qualitative and quantitative parameters of the feed made from the grassland sward (dry matter digestibility, dry matter yield, and protein yield) were used as dependent variables. Nine deterministic models were proposed Y_G, DIG_G, P_G, Y_GB, DIG_GB, P_GB, Y_GC, DIG_GC, and P_GC, which differed in the input variable and the main factor from the grassland experiment. The analysis of the sensitivity of the neural networks in the models enabled the identification of the independent variables with the greatest influence on the yield of dry matter and protein as well as the digestibility of the dry matter of the first regrowth of grassland sward, taking its diverse botanical composition into account. The results showed that the following factors were the most significant (rank 1): the average daily air temperature, total rainfall, and the percentage of legume plants. This research will be continued on a larger group of factors influencing the output variables and it will involve an attempt to optimise these factors.
Monitoring Coffee Leaf Rust (Hemileia vastatrix) on Commercial Coffee Farms in Hawaii: Early Insights from the First Year of Disease Incursion
Authors: Aristizábal Luis F. , Johnson Melissa A.
Published: May 2022

Coffee leaf rust (CLR, Hemileia vastatrix) is considered the most damaging coffee disease worldwide, causing reduced yields and even plant death. CLR was detected in Hawaii for the first time in 2020, and quickly spread across the state. We initiated a CLR monitoring program in Kona, West Hawaii Island, to track the spread of this new invasive disease across a broad elevational gradient. The goals of the program were to assist growers in the early detection of CLR, to characterize patterns of disease incidence across the region, and to collect information on farm agronomics, management practices, and costs to apply fungicides, all of which can be used to develop Integrated Pest Management (IPM) strategies for this pathogen. We monitored 30 coffee lots in Kona, located between 204 and 875 m elevation. Average CLR incidence remained below 4% early in the season and increased to 36% during harvest. We observed no significant difference in CLR incidence between low-, mid- and high-elevation farms. A significant reduction in the number of leaves per branch was observed at the end of the harvest season, and a significant negative correlation was found between the number of leaves per branch and maximum CLR severity. Mean disease incidence and mean severity were observed to have a significant positive correlation. Incidence increased above threshold levels (5%), despite most growers applying preventative fungicides 3–10 times throughout the season, suggesting that improved coverage and timing of applications is needed along with the addition of systemic fungicides. Our study provides the first insights into CLR disease patterns under the unique and variable conditions under which Hawaiian coffee is grown, and will aid in the development of IPM programs that can be used to sustain Hawaii’s coffee industry under this new threat.
Global Changes in Cultivated Area and Breeding Activities of Durum Wheat from 1800 to Date: A Historical Review
Authors: Martínez-Moreno Fernando , Ammar Karim , Solís Ignacio
Published: May 2022

Durum wheat is grown globally on 13.5 million ha in 2020/2021, which amounts to 6.2% of the wheat area. It is assumed that in the past it was more important, but the extent of that importance is unknown. In this work, a historical estimation of the durum wheat area globally was carried out, based on data of the main cultivating countries. Many of the data from the earliest period were based on percentage to all wheat. During the nineteenth century, the percentage of durum wheat to all wheat globally was around 14–16%. However, throughout the 19th and beginning of the 20th century, in America (USA, Canada, Argentina), Asia (Russia, China, India), and Australia, new land was sown with bread wheat, and therefore the percentage of durum wheat fell steadily to 7–9% from 1950 to 2005, and to 6–7% since then. For many years, Russia was the country with more durum wheat cultivation, with around six million ha in the period 1910–1940. Turkey, Italy, Algeria, and India were also big historical players regarding cultivation of this crop. Currently, Canada, Algeria, Italy, and Turkey have the largest durum wheat acreage. The main breeding activities and the future of durum wheat are discussed.
Morphological and Biochemical Factors Associated with Constitutive Defense to Thrips in Alfalfa
Authors: Zhang Zhiqiang , Chen Qi , Sa Rula , Dai Rui , Shuang Shuang , Jiang Xiaohong , Liu Huijie , Tan Yao , Tang Fang , Temuer Buhe
Published: May 2022

Plants have evolved a series of inducible or constitutive defense mechanisms in response to herbivore attack. Constitutive plant defenses are morphological and biochemical traits of the plants themselves, regardless of the presence of herbivores. We bred an alfalfa variety (Caoyuan No.4) with high thrips resistance, but the mechanisms underlying Caoyuan No.4 resistance to thrips are not well understood. To explore the constitutive defense of Caoyuan No.4, the morphological and biochemical traits associated with constitutive defense to thrips in alfalfa were analyzed using a thrips-susceptible alfalfa accession (Caoyuan No.2) as a control. The results showed that Caoyuan No.4 had thicker palisade tissue and parenchyma tissue, wider collenchyma, phloem, cambium and lignin layer, and smaller epidermal cells and stomatal aperture compared to Caoyuan No.2. Moreover, Caoyuan No.4 showed more non-glandular trichomes in both leaves and stems, but less glandular trichomes and more wax in stems. In addition, the results of the widely targeted metabolomics analysis showed that metabolites related to flavonoid, isoflavonoid, flavone and flavonol biosynthesis, as well as cysteine and methionine metabolism, differed between CaoyuanNo.2 and Caoyuan No.4. These findings shed new light on the constitutive insect defense of plants associated with physical or biochemical traits and may provide convenient markers for breeding thrips-resistant alfalfa cultivars.
Hydrochemical Assessment of Water Used for Agricultural Soil Irrigation in the Water Area of the Three Morava Rivers in the Republic of Serbia
Authors: Pivić Radmila , Maksimović Jelena , Dinić Zoran , Jaramaz Darko , Majstorović Helena , Vidojević Dragana , Stanojković-Sebić Aleksandra
Published: May 2022

The assessment of the suitability and status of irrigation water quality from the aspect of its potential negative impact on soil salinization and mapping of spatial distribution within the area of the three Morava rivers, which includes the South, West, and Great Morava basins, was the purpose of this research. A total of 215 samples of irrigation water were tested, and their quality was evaluated based on the analysis of the following parameters: pH, electrical conductivity (EC), total dissolved salt (TDS), sodium adsorption ratio (SAR), and content of SO42−, Cl−, HCO3−, CO3 2−, Mg2+, Ca2+, Na+, and K+. The results showed that the average content of ions was as follows: Ca2+ > Mg2+ > Na+ > K+ and HCO3− > SO42− > Cl− > CO32−. The assessment of irrigation water suitability was determined by calculating the following indices: percentage sodium (Na %), residual sodium carbonate (RSC), permeability index (PI), magnesium hazard (MH), potential salinity (PS), Kelley’s index (KI), total hardness (TH), irrigation water quality index (IWQI). Based on Wilcox’s diagram, the USSL diagram, and the Doneen chart, it was concluded that most of the samples were suitable for irrigation. Using multivariate statistical techniques and correlation matrices in combination with other hydrogeochemical tools such as Piper’s, Chadha’s, and Gibbs diagrams, the main factors associated with hydrogeochemical variability were identified.
Sex Differences in Mate Choice Preference Characteristics of Aequidens rivulatus
Authors: Li Haixia , Wang Jie , Zhang Xu , Hu Yu , Cai Qinglin , Liu Ying , Ma Zhen
Published: May 2022

The mating roles of males and females, to a certain extent, are dynamic and variable. Several factors influence the mate choice process. Nonetheless, the main preference features have not yet been fully understood in Aequidens rivulatus. In this study, because of its natural pairing characteristics, A. rivulatus was selected to explore the mate choice preferences of different sexes. Specifically, male and female behavioral performances were described and quantified through a “no-choice paradigm” during mate choice. A total of 12 behavioral performances were defined in male mate choice (experiment 1), whereas 14 behavioral performances were defined in female mate choice (experiment 2). According to the obtained results, unselected females did not display any proactive behaviors in experiment 1, whereas unselected males exhibited proactive behaviors in experiment 2, including quivering, nipping, tail beating, swimming up and down, and aggression. It was also found that both male and female individuals tend to express dislike rather than like. Those behaviors with higher frequencies (e.g., quivering) often mean less energy expenditure, thus easier repeatability. Moreover, principal component analysis (PCA) was employed to extract and identify mate choice preference features. Preliminary results indicated that male preferences for a mate were mainly associated with body size, behavioral intention, and appearance, whereas the intensity of female preferences was in the order of body size, appearance, and behavioral intention. In addition, sex hormone levels were associated with mate choices.
Is the Hitchcock Story Really True? Public Opinion on Hooded Crows in Cities as Input to Management
Authors: Kövér László , Paládi Petra , Benmazouz Isma , Šorgo Andrej , Špur Natalija , Juhász Lajos , Czine Péter , Balogh Péter , Lengyel Szabolcs
Published: May 2022

In recent years, the Hooded crow (Corvus cornix) has become one of the most successful wild bird species in urban environments across Europe. Hooded crows can cause several problems in cities, including trash scattering, noise disturbance, and aggressive behavior toward humans or pets, and they can be potential vectors of pathogens. To find effective solutions, the public has to be involved in the decision-making process in urban planning management, managed by the city administration. In this study, we surveyed the attitude of people in Hungary towards crows and crow management by collecting information using an online questionnaire containing 65 questions published in 14 Facebook groups. We found that many people were familiar with corvid species and had personal experience with them. In most cases, these experiences were not negative, so the crows were not or only rarely perceived to cause problems to people, such as aggressive behavior, damage to cars or stealing something. Most respondents recognized that the presence of large numbers of hooded crows is a problem to be solved and acknowledged that they do not know how to resolve it. The majority of people expressed their interest in raising public awareness of crows but not in their management actions, which they believe should be implemented by experts. Most respondents preferred passive, harmless methods. More direct methods such as egg/chick removal from the nest, control by trapping, poisoned baits or firearms, or oral contraceptives were the least acceptable. These results express the difficulty in identifying a control method for managing hooded crow populations that is both acceptable to most people and effective at the same time. This study demonstrates the importance of involving public opinion in wildlife management and providing more information to citizens to reduce human-crow conflicts.
Anti-Müllerian Hormone Inhibits FSH-Induced Cumulus Oocyte Complex In Vitro Maturation and Cumulus Expansion in Mice
Authors: Yu Xue , Li Zan , Zhao Xinzhe , Hua Liping , Liu Shuanghang , He Changjiu , Yang Liguo , Davis John S. , Liang Aixin
Published: May 2022

Anti-Müllerian hormone (AMH) is secreted by the ovaries of female animals and exerts its biological effects through the type II receptor (AMHR2). AMH regulates follicular growth by inhibiting the recruitment of primordial follicles and reducing the sensitivity of antral follicles to FSH. Despite the considerable research on the actions of AMH in granulosa cells, the effect of AMH on the in vitro maturation of oocytes remains largely unknown. In the current study, we showed that AMH is only expressed in cumulus cells, while AMHR2 is produced in both cumulus cells and oocytes. AMH had no significant effect on COCs nuclear maturation, whereas it inhibited the stimulatory effects of FSH on COCs maturation and cumulus expansion. Moreover, AMH treatment effectively inhibited the positive effect of FSH on the mRNA expressions of Hyaluronan synthase 2 (Has2), Pentraxin 3 (Ptx3), and TNF-alpha-induced protein 6 (Tnfaip 6) genes in COCs. In addition, AMH significantly decreased the FSH-stimulated progesterone production, but did not change estradiol levels. Taken together, our results suggest that AMH may inhibit the effects of FSH-induced COCs in vitro maturation and cumulus expansion. These findings increase our knowledge of the functional role of AMH in regulating folliculogenesis.
Screening and Carrier Rate of Neuronal Ceroid Lipofuscinosis in Chihuahua Dogs in Japan
Authors: Pervin Shahnaj , Islam Md Shafiqul , Tada Naomi , Tsutsui Toshihiko , Rahman Mohammad Mahbubur , Yabuki Akira , Tacharina Martia Rani , Rakib Tofazzal Md , Maki Shinichiro , Yamato Osamu
Published: May 2022

Neuronal ceroid lipofuscinosis (NCL) is a group of rare lethal neurodegenerative lysosomal storage diseases that occur in a range of dog breeds, including Chihuahuas. Recently, a homozygous single base-pair deletion (c.846delT), which causes a frame shift generating a premature stop codon (p.Phe282Leufs13*) in the canine CLN7/MFSD8 gene, has been identified as a causative mutation for NCL in Chihuahuas. The objective of this study was to determine the frequency of the mutant allele and/or carrier rate of NCL in Chihuahuas in Japan using a newly designed real-time PCR assay. Samples of saliva were randomly collected from 1007 Chihuahua puppies during physical examinations prior to the transportation to pet shops. Screening results revealed a carrier rate of 1.29%, indicating a mutant allele frequency (0.00645) that is considered sufficiently high to warrant measures for the control and prevention of this lethal disease. The genotyping assay designed in this study could make a valuable contribution to the control and prevention of NCL.
Use of Gnawing Hay Blocks: Effects on Productive Performance, Behavior and Reactivity of Growing Rabbits Kept in Parks with Different Sex-Group Compositions
Authors: Birolo Marco , Trocino Angela , Zuffellato Andrea , Pirrone Fabrizio , Bordignon Francesco , Xiccato Gerolamo
Published: May 2022

To improve animal welfare in collective park housing systems, this study assessed the effects of the provision of gnawing hay blocks and the group composition (F: females, M: males, FM: mixed sex) on performance, behavior, and reactivity of 288 growing rabbits reared in 18 parks (16 rabbits/park) from 31 to 73 days of age. The presence of gnawing blocks inside the feeding area of the parks scarcely affected performance and budget time, but decreased the time spent in the resting area compared to parks without blocks (p < 0.001); it increased the time spent moving during the open field test (p < 0.05) and the rate of rabbits that approached the object in the novel-object test (p < 0.05). As for sex-group composition, the feed conversion ratio was lower in parks F and FM than in park M (p < 0.05). During the open-field test, FM rabbits spent more time moving (p < 0.05), whereas M rabbits displayed self-grooming for a longer time (p < 0.01). Results related to production, behavior, and reactivity indicate the provision of gnawing blocks for welfare improvement, but do not support the change from current mixed-sex to single-sex rearing.
Liver Receptor homolog-1 Regulates Apoptosis of Bovine Ovarian Granulosa Cells by Progestogen Receptor Signaling Pathway
Authors: Xu Dejun , Jiang Xiaohan , Wang Yukun , Song Shuaifei
Published: May 2022

The purpose of the present investigation was to assess the function of LRH-1 on GCs and the mechanisms involved. Here, LRH- was highly expressed in the bovine GCs of atretic follicles. Treatment with 50 μM of LRH-1 agonist (DLPC) significantly induced the expression of LRH-1 (p < 0.05). In particular, LRH-1 activation blocked the progestogen receptor signaling pathway via downregulating progesterone production and progestogen receptor levels (p < 0.05), but had no effect on 17 beta-estradiol synthesis. Meanwhile, LRH-1 activation promoted the apoptosis of GCs and increased the activity of caspase 3 (p < 0.05). Importantly, upregulating the progestogen receptor signaling pathway with progestogen could attenuate the LRH-1-induced proapoptotic effect. Moreover, treatment with progestogen decreased the activity of the proapoptotic gene caspase 3 and increased the expression of antiapoptotic gene Bcl2 in LRH-1 activated GCs (p < 0.05). Taken together, these results demonstrate that LRH-1 might be dependent on the progestogen receptor signaling pathway to modulate bovine follicular atresia.
Impact of the COVID-19 Pandemic on Primate Research and Conservation
Authors: Reuter Kim , Andriantsaralaza Seheno , Hansen Malene , LaFleur Marni , Jerusalinsky Leandro , Louis Edward , Ratzimbazafy Jonah , Williamson Elizabeth , Mittermeier Russell
Published: May 2022

There is evidence to suggest that the Coronavirus Disease 2019 (COVID-19) pandemic may hamper our achievement of the Sustainable Development Goals (SDGs). Here, we use non-human primates as a case study to examine the impacts of COVID-19 on the ability to achieve biodiversity conservation and management sustainability targets. We collected data through a survey of members of the IUCN SSC Primate Specialist Group from January to March 2022. Of the 93 experts that responded to our survey, we found that 39% had not been able to visit any of their field sites since March 2020, 54% said they had less funding available for their primate-related work, and only one out of ten said they had managed to achieve at least 76–100% of their planned primate-related work since March 2020. Six out of ten respondents (61%) felt that primate conservation efforts in protected areas were worse than before the onset of the COVID-19 pandemic and one-third (33%) felt hunting was happening more frequently than before. This study provides evidence of the impacts of COVID-19 on progress towards achieving the SDGs, and provides practical lessons learned for biodiversity conservation efforts moving forward.
Social Behavior and Group Formation in Male Asian Elephants (Elephas maximus): The Effects of Age and Musth in Wild and Zoo-Housed Animals
Authors: LaDue Chase A. , Vandercone Rajnish P. G. , Kiso Wendy K. , Freeman Elizabeth W.
Published: May 2022

Asian elephants are endangered, and the long-term viability of the species depends on integrative approaches to address the sustainability of in-situ and ex-situ populations. Growing evidence shows that male elephants exhibit extensive and flexible social behavior that rivals the complexity of that of females. Male elephant sociality is expected to change dramatically around the unique sexual state of musth. However, data related to male Asian elephant sociality is lacking. Here, we conducted complementary observations in Wasgamuwa National Park, Sri Lanka, and North American zoos of male Asian elephant social behavior. Age and musth status, along with other factors, were associated with variation in social behavior and group formation of males. In wild male elephants, both musth status and age impacted elephant associations within all-male and mixed-sex groups: non-musth elephants were generally sighted less often in mixed-sex groups as they aged, while the inverse occurred with musth elephants. Musth status interacted with age to predict the number of conspecifics with which a wild male elephant associated: younger males were observed with more females during non-musth (but the opposite was true during musth), and male elephants between 20 and 30 years were observed with the highest number of male conspecifics except during musth. Finally, we found variation in aggression, prosocial behavior, and submissive behavior was influenced by intrinsic (age and musth status) and extrinsic factors (group size and type) in similar ways in both populations; prosocial behavior was most common and was influenced by the number of conspecifics present (both populations), and age, group type, and musth status (zoo population), while aggression was rare, especially among older elephants. We suggest that longitudinal studies of this threatened species will be particularly helpful to promote the reproduction and conservation of Asian elephants in in-situ and ex-situ environments.
Performance, Blood Lipid Profile, and the Expression of Growth Hormone Receptor (GHR) and Insulin-like Growth Factor-1 (IGF-1) Genes in Purebred and Crossbred Quail Lines
Authors: Shafik Basant M. , Kamel Eman R. , Mamdouh Maha , Elrafaay Shimaa , Nassan Mohamed A. , El-Bahy Salah M. , El-Tarabany Mahmoud S. , Manaa Eman A.
Published: May 2022

The aim was to evaluate the performance, blood lipid profile, and the relative expression of growth-related genes in purebred white and brown quail lines and their crossbred lines. A total of 240 one-day-old Japanese quail chicks of white and brown line, their crossbred line (WBQ: male white × female brown), and reciprocal crossbred line (BWQ: male brown × female white) were divided into four equal groups (60 birds each). The white quail line showed significantly higher final body weight, daily gain, and feed intake compared with the other quail lines (p < 0.001). Meanwhile, both crossbred quail lines (WBQ and BWQ) showed significantly lower FCR compared with both purebred quail lines (p = 0.001). Both crossbred quail lines showed greater dressing percentages compared with both purebred quail lines (p = 0.038). The brown quail line showed significantly (p = 0.05) higher levels of serum triglycerides and VLDL compared with the white and BWQ lines. The WBQ crossbred line exhibited significantly higher mRNA expression of GHR and IGF-1 genes compared with other quail lines (p < 0.001). Both crossbred lines (WBQ and BWQ) exhibited negative heterosis percentages for body weight (−4.39 and −3.90%, respectively) and feed intake (−10.87 and −14.59%, respectively). Meanwhile, heterosis percentages for FCR (−6.46 and −9.25%, respectively) and dressing percentage (7.54 and 6.38%, respectively) were improved in both crossbred lines. The WBQ line showed high heterosis percentages for the expression of GHR and IGF-1 genes (52.28 and 88.81%, respectively). In conclusion, the WBQ line exhibited significantly greater dressing percentage and better FCR, as well as higher mRNA expression of GHR and IGF-1 genes. These results may be helpful to improve breeding programs and to develop commercial lines of meat-type Japanese quail.
Epidemiological Prevalence of Phenotypical Resistances and Mobilised Colistin Resistance in Avian Commensal and Pathogenic E. coli from Denmark, France, The Netherlands, and the UK
Authors: Mead Andrew , Billon-Lotz Candice , Olsen Rikke , Swift Ben , Richez Pascal , Stabler Richard , Pelligand Ludovic
Published: May 2022

Colistin has been used for the treatment of non-invasive gastrointestinal infections caused by avian pathogenic E. coli (APEC). The discovery of mobilised colistin resistance (mcr) in E. coli has instigated a One Health approach to minimise colistin use and the spread of resistance. The aim of this study was to compare colistin susceptibility of APECs (collected from Denmark n = 25 and France n = 39) versus commensal E. coli (collected from the Netherlands n = 51 and the UK n = 60), alongside genetic (mcr-1–5) and phenotypic resistance against six other antimicrobial classes (aminoglycosides, cephalosporins, fluoroquinolones, penicillins, sulphonamides/trimethoprim, tetracyclines). Minimum inhibitory concentration (MIC) values were determined using a broth microdilution method (EUCAST guidelines), and phenotypic resistance was determined using disk diffusion. Colistin MIC values of APEC were significantly lower than those for commensals by 1 dilution (p < 0.0001, Anderson-Darling test), and differences in distributions were observed between countries. No isolate carried mcr-1–5. Three phenotypically resistant isolates were identified in 2/62 APEC and 1/111 commensal isolates. Gentamicin or gentamicin–ceftriaxone co-resistance was observed in two of these isolates. This study showed a low prevalence of phenotypic colistin resistance, with no apparent difference in colistin resistance between commensal E. coli strains and APEC strains.
Routine Postoperative Antibiotic Prophylaxis Offers No Benefit after Hepatectomy—A Systematic Review and Meta-Analysis
Authors: Murtha-Lemekhova Anastasia , Fuchs Juri , Teroerde Miriam , Chiriac Ute , Klotz Rosa , Hornuss Daniel , Larmann Jan , Weigand Markus A. , Hoffmann Katrin
Published: May 2022

Background: Prophylactic antibiotics are frequently administered after major abdominal surgery including hepatectomies aiming to prevent infective complications. Yet, excessive use of antibiotics increases resistance in bacteria. The aim of this systematic review and meta-analysis is to assess the efficacy of prophylactic antibiotics after hepatectomy (postoperative antibiotic prophylaxis, POA). Method: This systematic review and meta-analysis were completed according to the current PRISMA guidelines. The protocol has been registered prior to data extraction (PROSPERO registration Nr: CRD42021288510). MEDLINE, Web of Science and CENTRAL were searched for clinical reports on POA in hepatectomy restrictions. A random-effects model was used for synthesis. Methodological quality was assessed with RoB2 and ROBINS-I. GRADE was used for the quality of evidence assessment. Results: Nine comparative studies comprising 2987 patients were identified: six randomized controlled trials (RCTs) and three retrospectives. POA did not lead to a reduction in postoperative infective complications or have an effect on liver-specific complications—post-hepatectomy liver failure and biliary leaks. POA over four or more days was associated with increased rates of deep surgical site infections compared to short-term administration for up to two days (OR 1.54; 95% CI [1.17;2.03]; p = 0.03). Routine POA led to significantly higher MRSA incidence as a pathogen (p = 0.0073). Overall, the risk of bias in the studies was low and the quality of evidence moderate. Conclusion: Routine POA cannot be recommended after hepatectomy since it does not reduce postoperative infection or liver-specific complications but contributes to resistance in bacteria. Studies into individualized risk-adapted antibiotic prophylaxis strategies are needed to further optimize perioperative treatment in liver surgery.
Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (Rhodiola rosea L.) Commercial Samples
Authors: Polumackanycz Milena , Konieczynski Pawel , Orhan Ilkay Erdogan , Abaci Nurten , Viapiana Agnieszka
Published: May 2022

The aim of the study was to compare the chemical composition of the water and hydromethanolic extracts of R. rosea commercial samples in relation to their biological activity. For this purpose, the HPLC method was used for the determination of eleven phenolic compounds and AAS/AES was used for determination of five essential elements. Moreover, the contents of total phenolic, total flavonoid, total phenolic acids, and L(+)-ascorbic acid were determined. The antioxidant activity was assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS radical scavenging activity, ferric-reducing/antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) assays, while the inhibitory activity against AChE and BChE enzymes was determined using Ellman’s method. The results showed that the hydromethanolic extracts of R. rosea were richer in phenolic compounds and showed higher antioxidant and neurobiological activity than the water extracts. However, the water extracts gave higher contents of determined elements. Among the individual phenolic compounds gallic acid (2.33 mg/g DW) and sinapic acid (386.44 µg/g DW) had the highest concentrations in the hydromethanolic and water extracts, respectively. Moreover, the most extracts were observed to be more efficient on BChE. Moreover, the correlation analysis indicated a high positive relationship between chemical composition and biological activity in both extracts of R. rosea.
Roles of Heme Oxygenase-1 in Neuroinflammation and Brain Disorders
Authors: Wu Yi-Hsuan , Hsieh Hsi-Lung
Published: May 2022

The heme oxygenase (HO) system is believed to be a crucial mechanism for the nervous system under stress conditions. HO degrades heme to carbon monoxide, iron, and biliverdin. These heme degradation products are involved in modulating cellular redox homeostasis. The first identified isoform of the HO system, HO-1, is an inducible protein that is highly expressed in peripheral organs and barely detectable in the brain under normal conditions, whereas HO-2 is a constitutive protein that is highly expressed in the brain. Several lines of evidence indicate that HO-1 dysregulation is associated with brain inflammation and neurodegeneration, including Parkinson’s and Alzheimer’s diseases. In this review, we summarize the essential roles that the HO system plays in ensuring brain health and the molecular mechanism through which HO-1 dysfunction leads to neurodegenerative diseases and disruption of nervous system homeostasis. We also provide a summary of the herbal medicines involved in the regulation of HO-1 expression and explore the current situation regarding herbal remedies and brain disorders.
Developmental and Early Life Origins of Hypertension: Preventive Aspects of Melatonin
Authors: Tain You-Lin , Hsu Chien-Ning
Published: May 2022

Hypertension represents a major disease burden worldwide. Abundant evidence suggests that hypertension can originate in early life. Adverse programming processes can be prevented by early life intervention—namely, reprogramming—to avoid developing chronic diseases later in life. Melatonin is an endogenously produced hormone with a multifaceted biological function. Although melatonin supplementation has shown benefits for human health, less attention has been paid to exploring its reprogramming effects on the early life origins of hypertension. In this review, first, we discuss the physiological roles of melatonin in pregnancy, fetal development, and the regulation of blood pressure. Then, we summarize the epidemiological and experimental evidence for the early life origins of hypertension. This is followed by a description of the animal models used to examine early melatonin therapy as a reprogramming strategy to protect against the early life origins of hypertension. A deeper understanding of the developmental programming of hypertension and recent advances in early melatonin intervention might provide a path forward in reducing the global burden of hypertension.
Synergistic Antiproliferation of Cisplatin and Nitrated [6,6,6]Tricycle Derivative (SK2) for a Combined Treatment of Oral Cancer Cells
Authors: Wang Sheng-Chieh , Yen Ching-Yu , Shiau Jun-Ping , Chang Meng-Yang , Hou Ming-Feng , Jeng Jiiang-Huei , Tang Jen-Yang , Chang Hsueh-Wei
Published: May 2022

SK2, a nitrated [6,6,6]tricycle derivative with an n-butyloxy group, showed selective antiproliferation effects on oral cancer but not on normal oral cells. This investigation assessed for the first time the synergistic antiproliferation potential of cisplatin/SK2 in oral cancer cells. Cell viability assay at 24 h showed that a low dose of combined cisplatin/SK2 (10 μM/10 μg/mL) provided more antiproliferation than cisplatin or SK2 alone. Cisplatin/SK2 triggered also more apoptosis inductions in terms of subG1 accumulation, annexin V, pancaspase, and caspase 3/8/9 measurements. Moreover, cisplatin/SK2 provided more oxidative stress and DNA damage in oral cancer cells than independent treatments. Oxidative stress inhibitors rescued the cisplatin/SK2-induced antiproliferation and oxidative stress generation. Moreover, cisplatin/SK2 induced more antiproliferation, apoptosis, oxidative stress, and DNA damage in oral cancer cells than in normal oral cells (S-G). In conclusion, low-dose cisplatin/SK2 combined treatment promoted selective and synergistic antiproliferation in oral cancer cells depending on oxidative-stress-associated responses.
Utility of Obesity Indicators for Predicting Hypertension among Older Persons in Limpopo Province, South Africa
Authors: Modjadji Perpetua , Salane Mulalo Caroline , Mokwena Kebogile Elizabeth , Mudau Tshimangadzo Selina , Mphekgwana Peter Modupi
Published: May 2022

In view of the epidemic proportions of obesity in South Africa and its relationship to cardiometabolic diseases, such as hypertension, a cross sectional study was conducted to investigate the utility of obesity indicators for predicting hypertension among older persons (≥60 years, n = 350) in the Limpopo Province of South Africa. The WHO STEPwise approach was used to collect data on demographic and lifestyle factors. Anthropometrics and blood pressure were measured according to the standard procedures. Receiver operating characteristic curves (ROC) were used to investigate and compare the ability of obesity indicators to predict overall hypertension and either increased systolic (SBP) or increased diastolic (DBP) blood pressure. The area under the ROC curve (AUC) was used to assess a certain indicator’s potential to predict overall hypertension and either increased SBP or increased DBP. Multivariate logistic regression analysis was used to determine the relationship of hypertension with obesity indicators. The mean age of the participants was 69 years (±SD = 7), and hypertension (46%), general obesity (36%) and abdominal obesity (57%) were prevalent among older persons. The obesity indicator body mass index (BMI) (AUC = 0.603 (0.52; 0.69)) was the best predictor of hypertension in older men. Waist circumference (WC) (AUC = 0.640 (0.56; 0.72)) and waist-to-height ratio (WHtR) (AUC = 0.605 (0.52; 0.69)) were better predictors of hypertension than BMI and waist-to-hip ratio (WHR) in older women. After adjustment for risk factors, only WC (AOR = 1.22 (1.16; 1.79)) was significantly associated with hypertension in older women, proposing WC as a screening tool for the prediction of hypertension in South African older women.
Evaluation of City–Industry Integration Development and Regional Differences under the New Urbanization: A Case Study of Sichuan
Authors: Gan Lu , Wei Lihong , Huang Shan , Lev Benjamin , Jiang Wen
Published: May 2022

As an important focus of China’s new urbanization construction, the policy of “city–industry integration” has played a key role in promoting the sustainable development of urban construction. This paper integrates the three major elements of social service, ecology and environment, and science and technology into the traditional indicator system of “industry, city, and people”. Firstly, the PSO-AHP-EM model was constructed to empower the indicators subjectively and objectively, and the degree of city–industry integration was sorted by the GRA-TOPSIS model. Secondly, the Gini coefficient and Theil index were used to further explore the non-equilibrium of city–industry integration development. Finally, an empirical study was conducted on 18 prefecture-level cities in Sichuan Province from 2010 to 2019, and their development laws were analyzed. The results show that: (1) The weights obtained by the PSO-AHP solution were better than the weights obtained by AHP. (2) The level of city–industry integration in Sichuan was not high, and most cities need to be further developed. (3) The regional difference of city–industry integration was relatively small, and the main source of the difference was Chengdu Plain Economic Zone. This paper puts forward some suggestions to provide scientific support for the evaluation of city–industry integration.
Flexural Analysis Model of Externally Prestressed Steel-Concrete Composite Beam with Nonlinear Interfacial Connection
Authors: Yan Wutong , Chen Liangjiang , Han Bing , Xie Huibing , Sun Yue
Published: May 2022

Interfacial slip effects and the unbonded phenomenon of external tendons are the key mechanical features of the externally prestressed steel-concrete composite beams (EPCBs). In this paper, an 8-node fiber beam element is built for the nonlinear analysis of the composite beam with interfacial slip effects. A multi-node slipping cable element is proposed for the simulation of external tendons. The derived formulations are programmed in OpenSees as newly developed element classes to be conveniently used for the flexural analysis of EPCBs. The effectiveness of the proposed model is fully verified against the experimental tests of simply supported and continuous beams and then applied to the parametric study. The results show that the increasing deviator spacing will significantly decrease the tendon effective depth at ultimate states and further decrease the flexural capacity. The larger effective depth is beneficial to the tendon stress increments and further improves the flexural capacity. The enhancement of interfacial shear connection degree will increase the structural capacity but the effects on the tendon stress increments and second-order effects were not monotonic.
Some FFT Algorithms for Small-Length Real-Valued Sequences
Authors: Majorkowska-Mech Dorota , Cariow Aleksandr
Published: May 2022

This paper proposes fast algorithms for computing the discrete Fourier transform for real-valued sequences of lengths from 3 to 9. Since calculating the real-valued DFT using the complex-valued FFT is redundant regarding the number of needed operations, the developed algorithms do not operate on complex numbers. The algorithms are described in matrix–vector notation and their data flow diagrams are shown.
Natural Language Processing and Machine Learning Supporting the Work of a Psychologist and Its Evaluation on the Example of Support for Psychological Diagnosis of Anorexia
Authors: Rojewska Katarzyna , Maćkowska Stella , Maćkowski Michał , Różańska Agnieszka , Barańska Klaudia , Dzieciątko Mariusz , Spinczyk Dominik
Published: May 2022

Objective: This study sought to address the use of computer-aided diagnosis and therapy for anorexia nervosa. This paper presents the means by which the use of natural language processing methods can augment the work of psychologists. Method: We evaluated this method based on its efficacy when diagnosing anorexia nervosa. Using natural language processing and machine learning, we developed methods for analyzing five basic emotions, analyzing a patient’s body perception, and detecting six potential areas of difficulties for computer support of psychological diagnosis of anorexia. We surveyed 43 psychologists to obtain feedback on these tools. Results: We evaluated efficacy in terms of patient relationship, substantive aspects of the diagnosis, and diagnostic procedures. In terms of patient relationship, we found a noticeable decrease in the patient’s resistance and better support in verifying the substantive scope of the diagnostic thesis. Discussion: The presented methods can be a supporting tool for monitoring the diagnostic process and increasing the degree of self-diagnosis and self-reflection by the patient. This tool can increase the accuracy of the diagnostic process by reducing patient resistance. This will increase access to the patient’s psychopathology.
MARL-Based Dual Reward Model on Segmented Actions for Multiple Mobile Robots in Automated Warehouse Environment
Authors: Lee Hyeoksoo , Hong Jiwoo , Jeong Jongpil
Published: May 2022

The simple and labor-intensive tasks of workers on the job site are rapidly becoming digital. In the work environment of logistics warehouses and manufacturing plants, moving goods to a designated place is a typical labor-intensive task for workers. These tasks are rapidly undergoing digital transformation by leveraging mobile robots in automated warehouses. In this paper, we studied and tested realistically necessary conditions to operate mobile robots in an automated warehouse. In particular, considering conditions for operating multiple mobile robots in an automated warehouse, we added more complex actions and various routes and proposed a method for improving sparse reward problems when learning paths in a warehouse with reinforcement learning. Multi-Agent Reinforcement Learning (MARL) experiments were conducted with multiple mobile robots in an automated warehouse simulation environment, and it was confirmed that the proposed reward model method makes learning start earlier even there is a sparse reward problem and learning progress was maintained stably. We expect this study to help us understand the actual operation of mobile robots in an automated warehouse further.
Positron Emission Tomography in Coronary Heart Disease
Authors: de Almeida José , Martinho Sofia , Gonçalves Lino , Ferreira Maria
Published: May 2022

With advances in scanner technology, postprocessing techniques, and the development of novel positron emission tomography (PET) tracers, the applications of PET for the study of coronary heart disease have been gaining momentum in the last few years. Depending on the tracer and acquisition protocol, cardiac PET can be used to evaluate the atherosclerotic lesion (plaque imaging) and to assess its potential consequences—ischemic versus nonischemic (perfusion imaging) and viable versus scarred (viability imaging) myocardium. The scope of this review is to summarize the role of PET in coronary heart disease.
Semi-Automatic Method of Extracting Road Networks from High-Resolution Remote-Sensing Images
Authors: Yang Kaili , Cui Weihong , Shi Shu , Liu Yu , Li Yuanjin , Ge Mengyu
Published: May 2022

Road network extraction plays a critical role in data updating, urban development, and decision support. To improve the efficiency of labeling road datasets and addressing the problems of traditional methods of manually extracting road networks from high-resolution images, such as their slow speed and heavy workload, this paper proposes a semi-automatic method of road network extraction from high-resolution remote-sensing images. The proposed method needs only a few points to extract a single road in the image. After the roads are extracted one by one, the road network is generated according to the width of each road and the spatial relationships among the roads. For this purpose, we use regional growth, morphology, vector tracking, vector simplification, endpoint modification, road connections, and intersection connections to generate road networks. Experiments on four images with different terrains and different resolutions show that this method has high extraction accuracy under different image conditions. The comparisons with the semi-automatic GVF-snake method based on regional growth also showed its advantages and potentiality. The proposed method is a novel form of semi-automatic road network extraction, and it significantly increases the efficiency of road network extraction.