bluuebunny/crossref_metadata_2025 huggingface dataset:
Sporadic Hemangioblastoma of the Kidney: A clinicopathologic study of 3 cases and review of the literature
Authors: Xu Yanmei , Ma Xuehua , Ma Yong , Li Juan , Zhang Renya , Li Xiaomei
Published: November 2020

Background: Hemangioblastoma is a benign tumor of unknown histogenesis that mainly occurs in the central nervous system (CNS) associated with von Hippel-Lindau (VHL) disease. Much attention has been paid to the renal hemangioblastoma, but there are still some challenges in the differential diagnosis.Case presentation: Here, we describe three cases of sporadic renal hemangioblastoma with no clinical features of VHL diseases. All the three patients (male: 2; female: 1) were 40-56 years old. In all cases, the tumors were surrounded by a thick fibrous capsule and well-demarcated from the surrounding renal parenchyma. Tumors were composed of sheets or nests of polygonal to short spindle tumor cells and a rich capillary network. In case 1 and case 3, the large polygonal tumor cells contained abundant pale or eosinophilic cytoplasm, and some of the cells possessed intracytoplasmic lipid vacuoles. In case 2, tumor cells were characterized by uniform size, mild, clear or lightly stained cytoplasm and typical "clear cell" appearance. In the views of immunohistochemistry, the polygonal stromal cells were strongly and diffusely positive for α-inhibin, NSE, S100 protein, and vimentin. CD10 and PAX8 were positive, while EMA and CK showed focally positive in case 3. CK8/18, HMB45, MelanA, CgA, Syn, SMA, Desmin and CD56 were all negative. CD34 and CD31 outlined the contours and distribution of vascular networks in tumors. Conclusions: Renal hemangioblastoma is rare and prone to be misdiagnosed. More attention should be paid to the morphological features and reasonable application of immunohistochemistry for the diagnosis of hemangioblastoma.
miR-200c/FUT4 axis prevents the proliferation of colon cancer cells by downregulating the Wnt/β-catenin pathway
Authors: Cong Jinchun , Yang Chuanjia , Xia Zhixiu , Gong Jian , Zhang Hong
Published: November 2020

BackgroundMicroRNA(miR)-200c has been widely reported to be involved in colon cancer progress. However, the mechanisms of miR-200c in regulating tumor metastasis and growth remain to be fully elucidated. This study aimed to investigate the mechanism of miR-200c targets fucosyltransferase 4 (FUT4) on the proliferation of colon cancer.MethodsThe miR-200c and FUT4 mRNA levels in LoVo and SW480 cells were measured by real-time quantitative polymerase chain reaction. Further, miR-200c mimic, FUT4 siRNA and FUT4 mimic were transfected into cells, separately. Cell counting kit-8, plate colony formation and transwell assays were used to analyse the cells biological behaviour.. Immunofluorescence was used to analyse the Ki-67 expression Moreover, the Wnt/β-catenin pathway-related proteins were detected by western blots. A double luciferase experiment was performed to confirm the relationship between miR-200c and FUT4. In vivo, tumour growth and Wnt/β-catenin pathway-related proteins were also analysed. ResultsIn vitro, the expression of miR-200c and FUT4 were negatively correlated in LoVo and SW480 cells (correlation coefficients were -0.9046 and -0.9236, respectively). MiR-200c overexpression inhibited the proliferation, migration and invasion of LoVo and SW480 cells by downregulating FUT4. The Ki67-positive cells and Wnt/β-catenin signalling pathway-related proteins were reduced in the miR-200c overexpression and FUT4 silencing groups. A dual luciferase reporting system identified FUT4 as the target of miR-200c. The results in vivo were further confirmed the foundation of cells study.ConclusionsIn summary, miR-200c overexpression inhibits proliferation of colon cancer targeting FUT4 to downregulate the Wnt/β-catenin pathway, which promises molecular targets to inhibit metastasis for colon cancer therapy.
ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc
Authors: Cheng Yuanyuan , Lin Lishuang , Li Xiaoyan , Lu Aiqi , Hou Chenjian , Wu Qian , Hu Xiaomu , Zhou Zhongwen , Chen ZhongQing , Tang Feng
Published: November 2020

Background: Triple-negative breast cancer (TNBC) is the most challenging breast cancer subtype to treat, because it is so aggressive with shorter survival. Chemotherapy remains the standard treatment due to the lack of specific and effective molecular targets. The aim of the present study is to investigate the potential roles of A Disintegrin and Metalloproteinase 10 (ADAM10) on TNBC cells and the effects of combining ADAM10 expression and neoadjuvant chemotherapy treatment (NACT) to improve the overall survival in breast cancer patients.Methods: Using a series of breast cancer cell lines, we measured the expression of ADAM10 and its substrates by quantitative real-time PCR assay (qRT-PCR) and western blot analysis. Cell migration and invasion, cell proliferation, drug sensitivity assay, cell cycle and apoptosis were conducted in MDA-MB-231 cells cultured with ADAM10 siRNA. The effect of ADAM10 down-regulation by siRNA on its substrates was assessed by western blot analysis. We performed immunohistochemical staining for ADAM10 in clinical breast cancer tissues in 94 patients receiving NACT.Results: The active form of ADAM10 was highly expressed in TNBC cell lines. Knockdown of ADAM10 in MDA-MB-231 cells led to a significant decrease in cell proliferation, migration, invasion and the IC50 value of paclitaxel and adriamycin, while induced cell cycle arrest and apoptosis. And these changes were correlated with down-regulation of Notch signaling, CD44 and cellular prion protein (PrPc). In clinical breast cancer cases, a high ADAM10 expression in pre-NACT samples was strongly associated with poorer response to NACT and shorter overall survival. Conclusions: These data suggest the previously unrecognized roles of ADAM10 in contributing to the progression and chemo-resistance of TNBC.
The Influence of Gingival Phenotype on the Morphology of the Maxillary Central Papilla
Authors: Belák Šimon , Žižka Radovan , Starosta Martin , Zapletalová Jana , Šedý Jiří , Štefanatný Michal
Published: November 2020

BackgroundPreservation of the interdental papilla is an essential part of the functional and esthetic rehabilitation of dental treatment. It has been described that thicker gingival tissues are more resistant to recession. The main objective of this investigation was to analyze whether a thin gingival phenotype represents a potential risk factor affecting interdental papilla fill, height, or width in an esthetic region between maxillary central incisors. The secondary goals were: 1) to analyze parameters describing the papilla – fill, height, width, and effect of papilla base width on the vertical papillary dimension; 2) to determine correlation between different non-invasive measurements of gingival thickness; 3) to compare both sexes.MethodsA total of 54 periodontally healthy students (20–30 years old) were included in the study. Gingival thickness was measured using Pirop Ultrasonic Biometer. Gingival phenotype was also assessed by gingival probe transparency. Papilla height and width were measured, and the degree of papilla recession was classified.ResultsNo significant relationship between papilla fill, height, width and gingival probe transparency or gingival thickness was found. Gingival thickness and gingival probe transparency showed a significant relationship (P < 0.001). There was a significant relationship between papilla height and papilla fill (P = 0.028). A papilla which filled the interdental space completely seemed to be shorter. A strong positive correlation between papilla height and papilla width was found (P <0.0001). The papilla between maxillary central incisors was significantly higher in males (P = 0.01).ConclusionThe appearance of the interdental papilla may be influenced by various factors. Within the limitations of this study, the results showed that the thin gingival phenotype alone is no potential risk factor affecting interdental papilla fill, height, or width. It seems that there may be some effect of papilla base width on its vertical dimension. Gingival probe transparency is a simple reliable method of assessment of gingival thickness with a threshold value of 1-mm gingival thickness between the thick and thin phenotypes.
Prediction of the VeriStrat test in first-line therapy of pemetrexed-based regimens for advanced lung adenocarcinoma patients
Authors: Jia Bo , Dong Zhi , Wu Di , Zhao Jun , Wu Meina , An Tongtong , Wang Yuyan , Zhuo Minglei , Li Jianjie , Wang Yang , Zhang Jie , Zhao Xinghui , Li Sheng , Li Junfeng , Ma Menglei , Chen Chen , Yang Xue , Zhong Jia , Chen Hanxiao , Wang Jingjing , Chi Yujia , Zhai Xiaoyu , Cui Song , Zhang Rong , Ma Qingwei , Fang Jian , Wang Ziping
Published: November 2020

Background：Although advanced non-squamous non-small cell lung cancer (NSCLC) patients have significantly better survival outcomes after pemetrexed based treatment, a subset of patients still show intrinsic resistance and progress rapidly. Therefore we aimed to use a blood-based protein signature (VeriStrat, VS) to analyze whether VS could identify the subset of patients who had poor efficacy on pemetrexed therapy. Methods：This study retrospectively analysed 72 advanced lung adenocarcinoma patients who received first-line pemetrexed/platinum or combined with bevacizumab treatment. Results：Plasma samples from these patients were analysed using VS and classified into the Good (VS-G) or Poor (VS-P) group. The relationship between efficacy and VS status was further investigated. Of the 72 patients included in this study, 35 (48.6%) were treated with pemetrexed plus platinum and 37 (51.4%) were treated with pemetrexed/platinum combined with bevacizumab. Among all patients, 60 (83.3%) and 12 (16.7%) patients were classified as VS-G and VS-P, respectively. VS-G patients had significantly better median progression-free survival (PFS) (Unreached vs. 4.2 months; P<0.001) than VS-P patients. In addition, the partial response (PR) rate was higher in the VS-G group than that in the VS-P group (46.7% vs 25.0%, P=0.212). Subgroup analysis showed that PFS was also significantly longer in the VS-G group than that in the VS-P group regardless of whether patients received chemotherapy alone or chemotherapy plus bevacizumab. Conclusions：Our study indicated that VS might be considered as a novel and valid method to predict the efficacy of pemetrexed-based therapy and identify a subset of advanced lung adenocarcinoma patients who had intrinsic resistance to pemetrexed based regimens. However, larger sample studies are still needed to further confirm this result.
Prognostic value of Glypican family genes in early-stage pancreatic ductal adenocarcinoma after pancreaticoduodenectomy and possible mechanisms
Authors: Liu Jun-qi , Liao Xi-wen , Wang Xiang-kun , Yang Cheng-kun , Zhou Xin , Liu Zheng-qian , Han Quan-fa , Fu Tian-hao , Zhu Guang-zhi , Han Chuang-ye , Su Hao , Huang Jian-lu , Ruan Guo-tian , Yan Ling , Ye Xin-ping , Peng Tao
Published: November 2020

Background: This study explored the prognostic significance of Glypican (GPC) family genes in patients with pancreatic ductal adenocarcinoma (PDAC) after pancreaticoduodenectomy using data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO).Methods: A total of 112 PDAC patients from TCGA and 48 patients from GEO were included in the analysis. The relationship between overall survival and the expression of GPC family genes as well as basic clinical characteristics was analyzed using the Kaplan-Meier method with the log-rank test. Joint effects survival analysis was performed to further examine the relationship between GPC genes and prognosis. A prognosis nomogram was established based on clinical characteristics and prognosis-related genes. Prognosis-related genes were investigated by genome-wide co-expression analysis and gene set enrichment analysis (GSEA) was carried out to identify potential mechanisms of these genes affecting prognosis.Results: In TCGA database, high expression of GPC2, GPC3, and GPC5 was significantly associated with favorable survival (log-rank P = 0.031, 0.021, and 0.028, respectively; adjusted P value = 0.005, 0.022, and 0.020, respectively), and joint effects analysis of these genes was effective for prognosis prediction. The prognosis nomogram was applied to predict the survival probability using the total scores calculated. Genome-wide co-expression and GSEA analysis suggested that the GPC2 may affect prognosis through sequence-specific DNA binding, protein transport, cell differentiation and oncogenic signatures (KRAS, RAF, STK33, and VEGFA). GPC3 may be related to cell adhesion, angiogenesis, inflammatory response, signaling pathways like Ras, Rap1, PI3K-Akt, chemokine, GPCR, and signatures like cyclin D1, p53, PTEN. GPC5 may be involved in transcription factor complex, TFRC1, oncogenic signatures (HOXA9 and BMI1), gene methylation, phospholipid metabolic process, glycerophospholipid metabolism, cell cycle, and EGFR pathway.Conclusion: GPC2, GPC3, and GPC5 expression may serve as prognostic indicators in PDAC, and combination of these genes showed a higher efficiency for prognosis prediction.
Clinical diagnosis in paediatric patients at urban primary health care facilities in southern Malawi: a longitudinal observational study
Authors: Gondwe Mtisunge Joshua , Henrion Marc YR , O’Byrne Thomasena , Masesa Clemens , Lufesi Norman , Dube Queen , Majamanda Maureen D , Makwero Martha , Lalloo David G , Desmond Nicola
Published: November 2020

Background: Despite health centres being the first point of contact of care, there are challenges faced in providing care to patients at this level. In Malawi, service provision barriers reported at this level included long waiting times, high numbers of patients and erratic consultation systems which lead to mis-diagnosis and delayed referrals. Proper case management at this level of care is critical to prevent severe disease and deaths in children. Objective: Adopting ETAT to improve ability to identify severe illness in children at primary health centre (PHC) level through comparison with secondary level diagnoses. Methods: We implemented ETAT mHealth algorithm at eight urban PHCs in Blantyre, Malawi between April 2017 and September 2018. Health workers and support staff were trained in mHealth ETAT. Stabilisation rooms were established and equipped with emergency equipment. All PHCs used an electronic tracking system to triage and track sick children on referral to secondary care, facilitated by a unique barcode. Support staff at PHC triaged sick children using ETAT Emergency (E), Priority (P) and Queue (Q) symptoms and clinician gave clinical diagnosis. The secondary level diagnosis was considered as a gold standard. We used statistical computing software R (v3.5.1) and used exact 95% binomial confidence intervals when estimating diagnosis agreement proportions.Results: Eight-five percentage of all cases where assigned to E (9.0%) and P (75.5%) groups. Pneumonia was the most common PHC level diagnosis across all three triage groups (E, P, Q). The PHC level diagnosis of trauma was the most commonly confirmed diagnosis at secondary level facility (85.0%), while a PHC diagnosis of pneumonia was least likely to be confirmed at secondary level (39.56%). The secondary level diagnosis least likely to have been identified at PHC level was bronchiolitis 3 (5.2%). The majority of bronchiolitis cases (n = 50; (86.2%) were classified as pneumonia at the PHC level facility.Conclusions Implementing a sustainable and consistent ETAT approach with stabilisation and treatment capacity at PHC level reinforce staff capacity to diagnose and has the potential to reduce other health system costs through fewer, timely and appropriate referrals.
Virulent secondary metabolites of entomopathogenic bacteria genera, Xenorhabdus and Photorhabdus, inhibit phospholipase A2 to suppress host insect immunity
Authors: Mollah Md. Mahi Imam , Kim Yonggyun
Published: November 2020

Background: Xenorhabdus and Photorhabdus are entomopathogenic bacteria that cause septicemia and toxemia in insects. They produce secondary metabolites to induce host immunosuppression. Their metabolite compositions vary among bacterial species. Little is known about the relationship between metabolite compositions and the bacterial pathogenicity. The objective of this study was to compare pathogenicity and production of secondary metabolites of 14 bacterial isolates (species or strains) of Xenorhabdus and Photorhabdus. Results: All bacterial isolates exhibited insecticidal activities after hemocoelic injection to Spodoptera exigua (a lepidopteran insect) larvae, with median lethal doses ranging from 168.8 to 641.3 CFU per larva. Bacterial infection also led to immunosuppression by inhibiting eicosanoid biosynthesis. Bacterial culture broth was fractionated into four different organic extracts. All four organic extracts of each bacterial species exhibited insecticidal activities and resulted in immunosuppression. These organic extracts were subjected to GC-MS analysis which predicted 182 compounds, showing differential compositions for 14 bacteria isolates. There were positive correlations between total number of secondary metabolites produced by each bacterial culture broth and its bacterial pathogenicity based on immunosuppression and insecticidal activity. From these correlation results, 70 virulent compounds were selected from secondary metabolites of high virulent bacterial isolates by deducting those of low virulent bacterial isolates. These selected virulent compounds exhibited significant immunosuppressive activities by inhibiting eicosanoid biosynthesis. They also exhibited relatively high insecticidal activities.Conclusion: Virulence variation between Xenorhabdus and Photorhabdus is determined by their different compositions of secondary metabolites, of which PLA2 inhibitors play a crucial role.
CircTP63 promotes hepatocellular carcinoma progression by sponging miR-155-5p and upregulating ZBTB18
Authors: Wang Jiantao , Che Jinbiao
Published: November 2020

Background: Hepatocellular carcinoma (HCC) is the leading cause of tumor-related death worldwide due to high morbidity and mortality, yet lacking effective biomarkers and therapies. Circular RNAs (circRNAs) are a group of non-coding RNAs that regulate gene expression through interacting with miRNAs, implicating in the tumorigenesis and progression. A novel circRNA, circTP63, was reported to be an oncogene in HCC. However, its role in HCC remains unclear.Methods: qRT-PCR was used to assess the mRNA levels of CircTP63 in 90 pairs of tumor and adjacent normal tissues from HCC patients, one human normal hepatic epithelial cell line and HCC cell lines. CCK-8, colony formation, transwell, and flow cytometry assays were performed to detect the cellular function of circTP63/miR-155-5p/ZBTB18 in HCC cells. HCC xenograft mice models were established to assess the in vivo effect of circTP63. Bioinformatic analysis, RNA pull-down and luciferase assays were used to determine the interaction among circTP63/miR-155-5p/ZBTB18.Results: circTP63 was significantly upregulated in HCC tissues and cell lines. High circTP63 expression is closely associated with the tumor stages, lymph node metastasis, and poor prognosis of HCC patients. Functionally, knockdown of circTP63 inhibited cell proliferation, migration, invasion, and promoted cell apoptosis of HCC. Meanwhile, overexpression of circTP63 enhanced HCC progression. Mechanically, circTP63 was a sponge of miR-155-5p to facilitate the ZBTB18 expression, and the ZBTB18 expression in HCC tissues was negatively associated with the survival rate of HCC patients. Furthermore, rescued assays revealed that the reduced tumor-promoting effect on HCC cells induced by knockdown of circTP63 can be reversed by miR-155-5p inhibitor or ZBTB18 overexpression.Conclusion: Our data highlight a critical circTP63-miR-155-5p-ZBTB18 regulatory network involved in the HCC progression, gaining mechanistic insights into the function of circRNAs in HCC progression, and providing effective biomarkers and therapeutic targets for HCC treatment.
Mesenchymal stem cell–derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation
Authors: Xuemei Hu , Shen Nan , Liu Anqi , Wang Weidong , Zhang Lihua , Sui Zhigang , Tang Qingzhu , Du Xiangning , Yang Ning , Ying Wantao , Qin Biaojie , Li Zhitong , Li Lin , Wang Nan , Lin Hong-Li
Published: November 2020

Renal interstitial fibrosis (RIF) is an incurable pathological lesion in progressive chronic kidney diseases. Myofibroblast proliferation and microvascular damage are two important events in RIF, and pericytes are a major source of myofibroblasts in the kidney. However, the underlying mechanisms remain poorly characterized. We report that core fucosylation (CF), a post-translational modification of proteins, is essential for pericyte activation by regulating profibrotic and antifibrotic signaling pathways as a “hub-like” target. Mesenchymal stem cell (MSC)-derived exosomes reside specifically in the obstructed kidney and deliver microRNA (miR)-34c-5p to inhibit CF, reducing pericyte activation and renal fibrosis. Furthermore, we clarify that the CD81–EGFR ligand–receptor complex aids the entry of exosomal miR-34c-5p into pericytes. Our results reveal a novel mechanism of pericyte activation based on CF and suggest a potential use of MSC exosomes as a new therapeutic strategy for RIF by inhibiting CF, the hub-like target of profibrotic signaling activation.
A gapless unambiguous RNA metagenome-assembled genome sequence of a unique SARS-CoV-2 variant encoding spike S813I and ORF1a A859V substitutions
Authors: Soliman May Sherif , AbdelFattah May , Aman Soad M. N. , Ibrahim Lamyaa M. , Aziz Ramy Karam
Published: November 2020

The novel severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is causing an unprecedented pandemic, threatening global health, daily life, and economy. Genomic surveillance continues to be a critical effort towards tracking the virus and containing its spread, and more genomes from diverse geographical areas and different time points are needed to provide an appropriate representation of the virus evolution. We here report the successful assembly of one single gapless, unambiguous contiguous sequence representing the complete viral genome from a nasopharyngeal swab of an infected healthcare worker in Cairo, Egypt. The sequence has all typical features of SARS-CoV-2 genomes, with no protein-disrupting mutations; however, three mutations are worth highlighting and future tracking: a synonymous mutation causing a rare spike S-813-I variation) and two less frequent ones leading to an A41V variation in NSP3, encoded by ORF1a (ORF1a A895V), and a Q677H variation in the spike protein. Both affected proteins, S and NSP3, are relevant to vaccine and drug development. While the genome, named CU_S3, belongs to the prevalent global genotype, marked by the D614G spike variation, the combined variations in the spike proteins and ORF1a have not been observed in any of the 197,000 genomes reported to date. Future studies will assess the biological, pathogenic, and epidemiologic implications of this set of genetic variations.
Psychological factors influencing the feeling of loneliness in female students with hearing impairments
Authors: Baranauskienė Ingrida , Kovalenko Alla , Leonova Inna
Published: November 2020

The article presents the study results on the factors influencing the emergence of the feeling of loneliness in female students with hearing impairments. The results obtained indicate the presence of the feeling of loneliness in students with hearing impairments, especially, this feeling emerges most often in the family sphere. The feeling of loneliness is associated not only with interpersonal relationships, but also with personal traits, attitudes, needs. The sources of irritability and emotional instability for female students with hearing impairments are: insufficient satisfaction of their need for social and cultural communication, existing gender conflicts between partners, which are caused by social stereotypes and gender inequality. The main reasons for the feeling of loneliness in female students with hearing impairments are fears: of loneliness, dependence, rejection by others, both in interpersonal and social relations, as well as social anxiety. The feeling of loneliness emerges under the influence of the following factors: fear of communication, interpersonal relationships, personal characteristics, as well as dissatisfaction with one’s own life quality. Female students with hearing impairments require special attention from university staff to integrate them into the educational environment, establish contacts with peers and family members.
INFLUENCE OF EXOCRINE PANCREATIC INSUFFICIENCY ON THE COURSE OF TYPE 2 DIABETES MELLITUS IN PATIENTS WITH PRIMARY OSTEOARTHRITIS
Authors: Babinets Liliya , Halabitska Iryna
Published: November 2020

The study of the functional capacity of the pancreas in chronic pancreatitis (CP) in comorbidity with type 2 diabetes mellitus (DM2) and primary osteoarthritis (OA) is also relevant given that patients with this combination are associated with the presence of metabolic syndrome. The aim of the study was to investigate the state of functional capacity of the pancreas in patients with comorbid chronic pancreatitis and type 2 diabetes mellitus with osteoarthritis. Material and methods. 117 patients with CP with OA with concomitant DM2 and without it were studied. The main group consisted of 92 outpatients with CP with OA in combination with diabetes in the phase of stable or unstable remission, the comparison group – 25 patients with isolated CP with primary OA, and the control group – 30 healthy individuals. Diagnoses of OA, CP and DM2 were established according to generally accepted criteria and protocols Results. Analysis of the data showed that on average in the contingent of studied patients with comorbid OA, CP and DM2 excretory insufficiency of pancreas was significantly more severe than in the group of patients with isolated CP with OA. Conclusions. In the combined course of CP and DM2 with OA, the correlation between fecal α-elastase-1 and glycosylated hemoglobin was statistically significantly stronger than in the group of patients with isolated CP, indicating a mutually aggravating effect of CP and DM2.
Assessment of Groundwater Geochemistry of Vel River basin, Western Maharashtra, India
Authors: Gaikwad S. K. , Pathan N. D. , Bansode N. S. , Gaikwad S. P. , Badhe Y. P. , Naik T. S. , Sawant S. V.
Published: November 2020

To study the chemistry of major ion in groundwater from Vel (Velu) River basin, sixty (60) samples of dug wells and bore wells were collected and analyzed using standard techniques given by APHA. It shows order of dominance for cations, Na+ > Ca2+ > Mg2+ > K+ and in anionic concentration as HCO3- > Cl- > SO42- in groundwater. The pH of groundwater is slightly alkaline (range: pH 7.0 - 8.1), while average values of Electrical Conductivity (EC) is about 2641 µS/cm indicating high mineralization of groundwater. In general, the cationic concentration (Na+, K+, Ca2+ and Mg2+) of the groundwater increase in the downstream side (from Northwest to South east), suggesting geological control on the composition of groundwater while highest concentration is in lower part of the basin are generally associated with the high salinity. In the major anions, bicarbonate (HCO3-) is higher due to rock-water interaction. Average value of chloride is about of 235 mg/L due to discharge zones along with anthropogenic activities. The geochemical data plotted on Piper Trilinear Diagram is showing dominant hydro-chemical facies: Ca2++Mg2+, Na++ K+, Cl-+ SO42- -HCO3- found in 83.3 % samples indicating the alkaline earth exceeding the alkalis and the strong acids exceeds the weak acids. The pH, Total Hardness (TH) and Magnesium (Mg2+) of the samples show more proportion of samples falling above desirable limit. Otherwise the quality of groundwater is good for drinking. The irrigation indices like SAR, KR and SSP were considered to evaluate groundwater suitability for irrigation. Comparing with SAR parameter all samples are excellent to good for irrigation. In SSP, 33.3 % samples are within permissible, while 66.6% samples are doubtful for irrigation purpose. In KR almost all samples (excluding 04 samples in lower side of basin) are suitable for irrigation. So, variations in climate, geology with anthropogenic activities are modifying the groundwater geochemistry of Vel River Basin.
REPERCUSSÕES DO PRECEDENTE, ATIVISMO JUDICIAL E O CASO CONCRETO EM MATÉRIA AMBIENTAL: DISCUSSÃO E JULGAMENTO SOBRE A SITUAÇÃO DO AMIANTO COMO UMA INOVAÇÃO LEGISLATIVA
Authors: Cruz Álvaro Ricardo De Souza , Cabral Ana Luiza Novais
Published: November 2020

O ativismo judicial sempre foi motivo de embates entre grandes doutrinadores no Brasil e no mundo. O presente artigo realiza conjecturas do caso emblemático no direito ambiental brasileiro sobre o amianto, no qual a Corte declarou a inconstitucionalidade da Lei Federal 9.055/1995 que permitia o uso controlado do material em todo o país mesmo que a inconstitucionalidade não tenha sido o objeto da presente ação. Tendo em vista que o material é maléfico para o meio ambiente bem como para a saúde humana, o ativismo realizado pelo Supremo Tribunal Federal foi entendido como um benefício social. Assim, utilizando-se de uma metodologia teórico-jurídico com raciocínio dedutivo, através de análise doutrinária e jurisprudencial serão observados importantes fatores que influenciam diretamente no enaltecimento do Poder Judiciário brasileiro e o proveitoso julgamento no caso concreto do amianto no Brasil como um precedente para realização de novos casos de ativismo judicial como uma inovação legislativa às avessas.
DA MUTAÇÃO CONSTITUCIONAL AO ATIVISMO JUDICIAL: UMA ANÁLISE DO ENTENDIMENTO DO SUPREMO TRIBUNAL FEDERAL SOBRE PRISÃO EM SEGUNDA INSTÂNCIA
Authors: Sousa Andréia Carvalho de , Leitão Macell Cunha
Published: November 2020

O discurso de combate à criminalidade presente na esfera pública brasileira tem fomentado o debate sobre a admissibilidade da prisão após a condenação em segunda instância e, por conseguinte, a problemática jurídica acerca do princípio constitucional da presunção de inocência. Diante desse contexto, o presente trabalho buscou investigar os limites à mutação constitucional para verificar em que medida a interpretação do Supremo Tribunal Federal que flexibiliza a garantia fundamental de presunção de inocência consiste numa prática de ativismo judicial. Para tanto, foi realizada revisão bibliográfica sobre o tema, bem como a análise dos Habeas Corpus 126.292 e 152.752 e das Ações Declaratórias de Constitucionalidade 43 e 44. A investigação demonstrou que a mutação constitucional que autoriza a prisão antes do trânsito em julgado de sentença penal condenatória consiste em uma perversão de um instituto que busca preservar a supremacia e a força normativa da Constituição. Nesse caso, evidenciada enquanto prática de ativismo judicial que extrapola os limites interpretativos do texto normativo, a mutação se revela inconstitucional, pois contrária ao núcleo fundamental do Estado Democrático de Direito. Os resultados desse estudo implicam na crítica aos argumentos consequencialistas que levam à suspensão de direitos e garantias constitucionais por razões de segurança e de efetividade.
MULTIPARENTALIDADE E A EFETIVIDADE DO DIREITO DA PERSONALIDADE AOS ALIMENTOS: UMA ANÁLISE A PARTIR DA VISÃO DO SUPREMO TRIBUNAL FEDERAL N. RE 898.060
Authors: Siqueira Dirceu Pereira , Lima Henriqueta Fernanda C.A.F.
Published: November 2020

A Constituição Federal veio não só inaugurar uma nova constitucional, mas atribuir à dignidade da pessoa humana a função de irradiar seus efeitos para todo o ordenamento jurídico, inclusive, às relações privadas, até então norteadas pelo patrimonialismo. No âmbito do Direito das Famílias não foi diferente: o modelo tradicional de família, decorrente do matrimonio, cedeu espaço a novas configurações familiares, em que a multiparentalidade passou a ser uma realidade, exigindo dos julgadores interpretação para além das regras. Dentro dessa perspectiva, o afeto passa a ser elemento essencial, de modo que a filiação socioafetiva passa a ter preponderância ao critério meramente biológico, admitindo-se a existência concomitante do pai/mãe sanguíneo com o afetivo, exsurgindo o debate doutrinário e jurisprudencial quanto aos efeitos patrimoniais dessa relação. Nesse viés, o presente estudo se dedicará a analisar, especificamente, tomando como base os princípios da dignidade da pessoa humana, da solidariedade, da afetividade e da paternidade responsável, o instituto dos alimentos, aqui considerando como direito da personalidade, apontando as controvérsias, as implicações práticas e a efetividade, com o fito de compreender como os alimentos podem ser resguardados nessa nova formatação de familiar sem se incorrer em insegurança jurídica e enriquecimento indevido. Para tanto, utilizar-se-á do método dedutivo, de pesquisa teórica e da metodologia de análise de decisões, especialmente do julgado emblemático do Supremo Tribunal Federal (STF) no Recurso Extraordinário (RExt) 898.060 por ter sedimentado o debate quanto à pluriparentalidade, sem contudo, ter adentrado à análise dos limites, alcance e, sobretudo, os impactos jurídicos noutros institutos.
O ECOTURISMO E A PLANIFICAÇÃO DA GESTÃO AMBIENTAL NO DESENVOLVIMENTO DE ESTRATÉGIAS EM ÁREAS NATURAIS PROTEGIDAS
Authors: Gomes Magno Federici , Gonçalves Joana Rita Gomes
Published: November 2020

Com o presente artigo, pretende-se discorrer acerca da importância do ecoturismo, enquanto turismo sustentável preservacionista dos recursos naturais, biodiversidade e vida selvagem, nos seus impactos e benefícios face à necessidade preservacionista do ambiente natural. O turismo sustentável envolve as comunidades locais e a sua efetiva participação na proteção dos patrimónios natural, cultural e histórico e, no desenvolvimento de estratégias e planejamento para que se alcancem as dimensões social, ambiental e econômica da sustentabilidade. A metodologia de pesquisa utilizada será bibliográfica e documental de raciocínio dedutivo.
Alocação de alunos por turmas em rede municipal de ensino: percepções de diretores escolares
Authors: Martins Angela Maria , Rosa Sanny , Sousa Sandra Maria Zákia Lian
Published: November 2020

Este artigo discute quais são os mecanismos de seleção adotados por diretores de escolas municipais para acolhimento e alocação de alunos por turmas e que podem, eventualmente, acentuar diferenças e desigualdades no processo de escolarização. Procedeu-se a uma dinâmica de grupo com a participação de 18 diretores de escolas e 5 assessoras da Secretaria de Educação Municipal de um município da Região Metropolitana de São Paulo. Os dados sinalizam as práticas que preponderam na rede municipal: matrícula por proximidade da rede física escolar; autoavaliações docentes; preferências docentes.
A atuação, no cenário da escola, limitada por práticas de gestão empresarial
Authors: Motta Henri De Leão , Araujo Jair Jonko
Published: November 2020

Este artigo objetiva discutir a atuação de professoras de uma escola estadual rural de ensino médio do município de Pelotas/RS no processo de redesenho curricular da política do Ensino Politécnico. Ajustado ao paradigma qualitativo, fundamenta-sena teoria da atuação de Stephen Ball, a produção de dados decorreu do exame documental de e-mails da escola relacionados à construção desta política e do debate, em um grupo focal, com cinco professores, cujo processo de análise foi organizado a partir da análise textual discursiva. Conclui-se que a política estimulou práticas de gestão empresarial da escola, imbricadas na burocratização e no controle das vozes internas.
Construcciones discursivas sobre el sujeto de la educación en la política educativa APRENDER
Authors: Falkin Camila , Farenzena Nalú
Published: November 2020

El artículo trata de la política Atención Prioritaria en Entornos con Dificultades Estructurales Relativas (APRENDER), de la educación primaria uruguaya. Se discuten los sentidos construidos sobre el sujeto de la educación en una política focalizada en niños que viven en contextos de pobreza, partiendo de la perspectiva del Análisis Político del Discurso y de un corpus conformado por documentos oficiales y entrevistas a técnicos. Se identifica una tensión entre una concepción que subraya las posibilidades educativas de los sujetos y otra que, tanto en los discursos de los años 90 como en los de la era progresista, los visualiza como seres carentes.
CRISES NA EDUCAÇÃO, REPRESENTAÇÃO E FORMAÇÃO DE PROFESSOR: UMA CONTRIBUIÇÃO DO PENSAMENTO COMPLEXO NA OBRA DE MORIN
Authors: Ferreira Maria de Fátima De Andrade , Santos Cinthia Maria Seibert
Published: November 2020

Este artigo é uma reflexão sobre crises na educação, representação e formação de professor, buscando destacar razões do mal-estar em tempos de crises contemporâneas e, de que modo as políticas públicas educacionais são implementadas nessa direção. Os desafios à formação docente são diversos, merecem tratamento rigoroso e devem fazer parte de discussões que nos retire da banalidade, autoritarismo, “achismos”, com que o conhecimento (MORIN, 2011; MOSCOVICI, 1978; CASTORIADIS, 2000) é tratado pela escola e setores responsáveis pela educação como direitos para todos brasileiros.
The Relationship of Parenting Style and Perception of Sexuality, Gender and Norm of Reproductive Health among Adolescents
Authors: Wulandari Eka Aprilia , Susanto Tantut , Nur Kholid Rosyidi Muhammad
Published: November 2020

Parenting is an interaction between children and parents, including fulfilling physical, psychological needs, and socializing norms in society. The results of the adaptation of parenting styles include sexuality, gender, and norm perception (SGNP) in adolescent reproductive health. This study aims to analyze the relationship between parenting style with SGNP in adolescent reproductive health in Patrang District, Jember Regency. This study was a correlational analytic with a cross-sectional approach conducted among 158 participants with a cluster random sam­pling technique. Data collection used a questionnaire to identify the characteristics of participants, parenting with Parenting Style Dimension Questionnaire (PSDQ), and SGNP in adolescent repro­duc­tive health with Sexuality, Gender, and Norms Questionnaire. Spearman Rank Correlation as an analysis method was used to answer the objectives of this study. Among 158 participants, 65.8% were authoritative parenting, 20.9% were authoritarian and 13.3% were permissive. Besides, 53.2% of adolescents had positive of SGNP in reproductive health. There is a relationship between parenting style and SGNP in adolescent reproductive health (r = 0.193; p = 0.015). Therefore, a planned intervention is needed such as educating parents and adolescents to be able to apply proper parenting to form positive perceptions.
Regulation Improvement of Right to Education Policy for Street Children in Padang City
Authors: Niswar Aurora Virgonia , Hanida Rozidateno Putri , Putera Roni Ekha
Published: November 2020

The fulfillment policy of the right to education for street children has been included in some policies in Padang City. However, it still hasn't been able to solve the problem. Hence, policy improvements are as impressive as research topics. The propose of this research is to describe and analyze the implementation process of education rights fulfillment for street children in Padang city. This research was conducted using qualitative research methods and descriptive design by employing library research and field research. The data were collected through interviews and documentation. The results of this research show that the policy needs improvement because there have been obstacles during the imple­mentation of the policy, which make the policy not function optimally.
Nicotinamide: Oversight of Metabolic Dysfunction Through SIRT1, mTOR, and Clock Genes
Authors: Maiese Kenneth
Published: November 2020

Metabolic disorders that include diabetes mellitus present significant challenges for
maintaining the welfare of the global population. Metabolic diseases impact all systems of the
body and despite current therapies that offer some protection through tight serum glucose control,
ultimately such treatments cannot block the progression of disability and death realized with
metabolic disorders. As a result, novel therapeutic avenues are critical for further development to
address these concerns. An innovative strategy involves the vitamin nicotinamide and the pathways
associated with the silent mating type information regulation 2 homolog 1 (Saccharomyces cerevisiae)
(SIRT1), the mechanistic target of rapamycin (mTOR), mTOR Complex 1 (mTORC1),
mTOR Complex 2 (mTORC2), AMP activated protein kinase (AMPK), and clock genes. Nicotinamide
maintains an intimate relationship with these pathways to oversee metabolic disease and improve
glucose utilization, limit mitochondrial dysfunction, block oxidative stress, potentially function
as antiviral therapy, and foster cellular survival through mechanisms involving autophagy.
However, the pathways of nicotinamide, SIRT1, mTOR, AMPK, and clock genes are complex and
involve feedback pathways as well as trophic factors such as erythropoietin that require a careful
balance to ensure metabolic homeostasis. Future work is warranted to gain additional insight into
these vital pathways that can oversee both normal metabolic physiology and metabolic disease.
An Overview of Ovarian Cancer: Molecular Processes Involved and Development of Target-based Chemotherapeutics
Authors: Shaik Basheerulla , Zafar Tabassum , Balasubramanian Krishnan , Gupta Satya P.
Published: November 2020

Ovarian cancer is one of the leading gynecologic diseases with a high mortality rate
worldwide. Current statistical studies on cancer reveal that over the past two decades, the fifth most
common cause of death related to cancer in females of the western world is ovarian cancer. In spite of
significant strides made in genomics, proteomics and radiomics, there has been little progress in transitioning
these research advances into effective clinical administration of ovarian cancer. Consequently,
researchers have diverted their attention to finding various molecular processes involved in the
development of this cancer and how these processes can be exploited to develop potential chemotherapeutics
to treat this cancer. The present review gives an overview of these studies which may update
the researchers on where we stand and where to go further. The unfortunate situation with ovarian
cancer that still exists is that most patients with it do not show any symptoms until the disease has
moved to an advanced stage. Undoubtedly, several targets-based drugs have been developed to treat
it, but drug-resistance and the recurrence of this disease are still a problem. For the development of
potential chemotherapeutics for ovarian cancer, however, some theoretical approaches have also been
applied. A description of such methods and their success in this direction is also covered in this review.
Coumarins as Potential Anti-drug Resistant Cancer Agents: A Mini Review
Authors: Wang Gangqiang , Sun Shaofa , Wu Bin , Liu Jikai
Published: November 2020

Cancer is the main cause of death, and the emergence of resistance by cancer cells towards
existing anticancer agents is one of the major problems for effective chemotherapy, creating a great
demand for the development of novel anticancer drugs. Coumarins, abundantly found in nature, constitute
an important class of pharmacological agents possessing physiological activities. The mechanism
investigations elucidated that coumarins could lead to self-programmed cancer cell death (apoptosis)
via different mechanisms; thereby coumarin-based derivatives possess potential activity against
various forms of cancers, including drug-resistant, even multidrug-resistant cancers. This review article
describes the recent progress in the discovery, structural modification, structure-activity relationship,
and mechanism of action studies of coumarin-based derivatives with potential activity against
drug-resistant cancers, covering articles published between 2010 and 2020.
Rectal Cancer in Patients Younger than 40: Tumor Characteristics and Comparative Survival Based on a Single Institution
Authors: Hilal Lara , Hakim Ayman , El-Chediak Alissar , Temraz Sally , Mukherji Deborah , El-Husseini Ziad , Shamseddine Ali
Published: November 2020

Aim:

The aim of this study was to determine tumor characteristics and outcomes of patients
with rectal cancer <40 years old compared to those above that age at a single institution in
Lebanon.

Background:

The incidence of colorectal cancer is increasing in younger adults with limited data from the Middle East.

Objective:

Overall survival (OS) and disease-free survival (DFS) were estimated using Kaplan-Meier.

Methods:

We conducted a retrospective study of patients diagnosed with rectal cancer over 15 years. Data were collected
regarding demographics, stage, pathology, treatment, and outcomes. Patients were stratified by age with 40 years as the
cut-off. Descriptive statistics were conducted.

Results:

Data for 105 cases were reviewed, 18 patients were aged under 40 years old and 87 patients
were above 40 years old. Younger patients had more poorly differentiated tumors than older
patients and were more likely to have tumors with signet-ring features. 5-year DFS was 35% and
51.5% for patients below and above 40 years old, respectively (P=0.04). OS was similar in the two
age groups, with a median follow-up of 36 months.

Conclusion:

Further prospective studies with a larger sample size and molecular markers are needed to better understand
the characteristics of rectal cancer in the young age group. With worse DFS in our study and emerging evidence of a
correlation between younger age at diagnosis and poor outcomes, consideration should be given to more personalized
upfront intensification of treatment in the young.
Forecasting of COVID-19 Cases in India: A Predictive Study
Authors: Sharma Pooja , Sharma Tanu , Veer Karan
Published: November 2020

An outbreak of new coronavirus (COVID-19) originated by SARS-CoV has reached
212 countries throughout the world. India is the second-highest populated country, so it is critical
to forecasting the confirmed cases and deaths due to pandemic. To fulfil the purpose, three machine
learning models Linear Regression, Multilayer Perceptron, and Sequential Minimal Optimization
Regression are used. The predictive data of three geographic regions (India, Maharashtra, and
Tamil Nadu) are compared with the data considered to be adequate in practice. The analysis concluded
that Sequential Minimal Optimization Regression can be adopted for possible pandemic predictions
such as COVID-19.