bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of High Energy Mining-Induced Seismic Shocks from Different Mining Activity Regions on a Multiple-Support Road Viaduct
Authors: Boroń Paweł , Dulińska Joanna Maria , Jasińska Dorota
Published: August 2020

In this paper, the dynamic responses of a large-scale multiple-support road viaduct to mining-induced seismic events registered in two regions of mining activity were compared. The regions differ in geological structure, which results in discrepancies in the dominant frequency content. Spatial variation of ground motion causing the kinematic excitation non-uniformity was accounted for in the dynamic analyses of this large-scale structure. Non-uniform mining-induced kinematic excitation models were proposed, with respect to the specificity of mining origin quakes. The dynamic performance of the viaduct was determined using three different methods of calculation: the time history analysis, the response spectrum analysis, and the multiple support response spectrum analysis. Both the uniform and non-uniform kinematic excitation models were adopted for the dynamic performance assessment. The research revealed that the dynamic response of some members of the structure, determined using the non-uniform excitation model, was significantly greater than that obtained for the uniform one. Hence, in the dynamic analysis of multiple-support structures under mining-induced events, the effect of spatial variation of ground motion should be considered. The study pointed out that the commonly used response spectrum analysis may lead to the underestimation of the dynamic response of large-scale multiple-support structures. Instead, the multiple support response spectrum method, which takes into account the non-uniformity of ground motion, is recommended as a conservative approximation. This method provides a safe upper estimation of the full-dynamic analysis results of large-scale structures under mining-induced tremors. Finally, the research indicated that the dynamic performance of a structure strongly depends on the frequency range attributed to a specific mining region. The dynamic performance of identical engineering structures under tremors of similar maximal amplitudes may differ significantly due to discrepancies in frequency contents of shocks occurring in various mining regions.
A GIS-Based Planning Approach for Urban Power and Natural Gas Distribution Grids with Different Heat Pump Scenarios
Authors: Kisse Jolando M. , Braun Martin , Letzgus Simon , Kneiske Tanja M.
Published: August 2020

Next to building insulation, heat pumps driven by electrical compressors (eHPs) or by gas engines (geHPs) can be used to reduce primary energy demand for heating. They come with different investment requirements, operating costs and emissions caused. In addition, they affect both the power and gas grids, which necessitates the assessment of both infrastructures regarding grid expansion planning. To calculate costs and CO2 emissions, 2000 electrical load profiles and 180 different heat demand profiles for single-family homes were simulated and heat pump models were applied. In a case study for a neighborhood energy model, the load profiles were assigned to buildings in an example town using public data on locations, building age and energetic refurbishment variants. In addition, the town’s gas distribution network and low voltage grid were modeled. Power and gas flows were simulated and costs for required grid extensions were calculated for 11% and 16% heat pump penetration. It was found that eHPs have the highest energy costs but will also have the lowest CO2 emissions by 2030 and 2050. For the investigated case, power grid investments of 11,800 euros/year are relatively low compared to gas grid connection costs of 70,400 euros/year. If eHPs and geHPs are combined, a slight reduction of overall costs is possible, but emissions would rise strongly compared to the all-electric case.
Optimization Wells Placement Policy for Enhanced CO2 Storage Capacity in Mature Oil Reservoirs
Authors: Kuk Michał , Kuk Edyta , Janiga Damian , Wojnarowski Paweł , Stopa Jerzy
Published: August 2020

One of the possibilities to reduce carbon dioxide emissions is the use of the CCS method, which consists of CO2 separation, transport and injection of carbon dioxide into geological structures such as depleted oil fields for its long-term storage. The combination of the advanced oil production method involving the injection of carbon dioxide into the reservoir (CO2-EOR) with its geological sequestration (CCS) is the CCS-EOR process. To achieve the best ecological effect, it is important to maximize the storage capacity for CO2 injected in the CCS phase. To achieve this state, it is necessary to maximize recovery factor of the reservoir during the CO2-EOR phase. For this purpose, it is important to choose the best location of CO2 injection wells. In this work, a new algorithm to optimize the location of carbon dioxide injection wells is developed. It is based on two key reservoir properties, i.e., porosity and permeability. The developed optimization procedure was tested on an exemplary oil field simulation model. The obtained results were compared with the option of arbitrary selection of injection well locations, which confirmed both the legitimacy of using well location optimization and the effectiveness of the developed optimization method.
Pyrolysis Kinetic Parameters of Omari Oil Shale Using Thermogravimetric Analysis
Authors: Abu El-Rub Ziad , Kujawa Joanna , Al-Gharabli Samer
Published: August 2020

Oil shale is one of the alternative energies and fuel solutions in Jordan because of the scarcity of conventional sources, such as petroleum, coal, and gas. Oil from oil shale reservoirs can be produced commercially by pyrolysis technology. To optimize the process, mechanisms and rates of reactions need to be investigated. Omari oil shale formation in Jordan was selected as a case study, for which no kinetic models are available in the literature. Oil shale was analyzed using the Fischer assay method, proximate analysis (moisture, volatile, and ash), gross calorific value, elemental analysis (CHNS), and X-ray fluorescence (XRF) measurements. Non-isothermal thermogravimetric analysis was applied to study the kinetic parameters (activation energy and frequency factor) at four selected heating rates (5, 10, 15, and 20 °C/min). When oil shale was heated from room temperature to 1100 °C, the weight loss profile exhibited three different zones: drying (devolatilization), pyrolysis, and mineral decomposition. For each zone, the kinetic parameters were calculated using three selected methods: integral, temperature integral approximation, and direct Arrhenius plot. Furthermore, the activation energy in the pyrolysis zone was 112–116 kJ/mol, while the frequency factor was 2.0 × 107 − 1.5 × 109 min−1. Moreover, the heating rate has a directly proportional relationship with the rate constant at each zone. The three different methods gave comparable results for the kinetic parameters with a higher coefficient of determination (R2) for the integral and temperature integral approximation compared with the direct Arrhenius plot. The determined kinetic parameters for Omari formation can be employed in developing pyrolysis reactor models.
Proposal of a System for Assessment of the Sustainability of Municipalities (Sasmu) Included in the Spanish Network of National Parks and Their Surroundings
Authors: Martínez-Vega Javier , Rodríguez-Rodríguez David , Fernández-Latorre Francisco M. , Ibarra Paloma , Echeverría Maite , Echavarría Pilar
Published: August 2020

It is usually considered that Protected Areas (PAs) are an efficient tool for policies to conserve biodiversity. However, there is evidence that some pressures and threats arise from processes taking place both inside them and in their surroundings territories—habitat loss, changes in land use, fragmentation of natural ecosystems. In this paper, we aim to test the hypothesis that municipalities located in the Socioeconomic Influence Zones (SIZs) of the fifteen National Parks (NPs) in Spain are more sustainable than those in their surroundings or, conversely, that the municipalities of their surroundings are more unsustainable. To measure their sustainability, we propose a system for assessment using fifteen indicators selected by experts. The methodology is based on the normalization of the data of each indicator, comparing them with a desirable target value defined in terms of sector policies and strategies. We then aggregate the indicators for each group in three indices that cover the classic dimensions of sustainability—environmental, economic and social. On a network scale, the results show that municipalities inside the SIZs are 1.594 points more sustainable environmentally, 0.108 economically and 0.068 socially than those of their surroundings. A system for assessment of the sustainability of municipalities (SASMU) may be a useful tool for NP managers, and for local and regional administrations, when setting priorities for policies, projects and compensation for regulatory restrictions related to NPs.
Investigating Connections between Need for Cognitive Closure and Climate Change Concern in College Students
Authors: Orr Margaret , Stewart Alan , Grundstein Andrew
Published: August 2020

Understanding how people’s worldviews and individual personality differences affect their thinking about anthropogenic climate change is critical to communication efforts regarding this issue. This study surveyed University of Georgia students to investigate the role that need for cognitive closure plays in level of climate change worry. The relationship between these two was found to involve suppression—a subset of mediation—by the social dimension of political conservatism. Political conservatism was also found to play a mediating role in the relationship between need for cognitive closure and support for governmental and personal climate solutions. However, social conservatism played this mediator role in women, and functioned as a suppressor for men. These findings help inform audience segmentation and creation of climate-related messages based on audience worldview and personality.
Quantifying the Impact of the Grain-for-Green Program on Ecosystem Health in the Typical Agro-Pastoral Ecotone: A Case Study in the Xilin Gol League, Inner Mongolia
Authors: Wang Zhaoyi , Yu Qianru , Guo Luo
Published: August 2020

The Green-for-Grain program (GGP) is the largest environmental restoration program in China. It is effective in controlling land desertification but at the same time is highly affected by regional differences. Ecosystem health, as an important indicator of ecosystem sustainability, can effectively assess the ecological impact of the GGP and provide a basis for follow-up actions. As a typical agro-pastoral ecotone along the Great Wall, the Xilin Gol League has seen increasing land-use intensity, thus, it is crucial to understand the ecological conditions of the region in order to deploy a policy of the GGP in accordance with local conditions. In this study, using remote sensing images and social statistics data from 1990–2015, land-use transformation and the turning point of vegetation coverage was determined. Based on the pressure-state-response (PSR) model, an ecological health evaluation system was constructed to quantify the temporal and spatial variation of ecosystem health. Then, the spatial correlation between the changes in forest and grass coverage, as well as the changes in the ecosystem health index (EHI), was evaluated using GeoDa software. The results showed that (1) grassland was the primary land-use/land-cover (LULC) in the Xilin Gol League. Since 2000, land-use transfer types changed significantly and grassland degradation weakened; landscape connectivity increased, and vegetation coverage increased. (2) Over the past 25 years, the ecosystem in the study area was at a subhealthy level and showed a trend toward a healthy level. (3) The spatial correlation between △Area% (change in forest and grass coverage) and △EHI (change in ecosystem health index) was positive between 2000 and 2015 and the correlation gradually increased, indicating that the GGP did enhance the health of the ecosystem of Xilin Gol. This study provided a specific reference for the evaluation of ecosystem health in the agro-pastoral ecotone of China and a theoretical basis for the implementation of sustainable management policies in the study area.
Secular Trends in Physical Fitness of Children and Adolescents: A Review of Large-Scale Epidemiological Studies Published after 2006
Authors: Eberhardt Tanja , Niessner Claudia , Oriwol Doris , Buchal Lydia , Worth Annette , Bös Klaus
Published: August 2020

Physical fitness (PF) of children and adolescents is an important resource for their future health. Population-based studies, however, rarely report secular changes of PF, although monitoring of these is crucial to deriving information for adequate interventions. This review aims to report trends in PF of children and adolescents. A literature search was conducted in PubMed in July 2019. Cohort studies published in English allowing statements to be made on trends in PF by comparing youth between the ages of four and 18 years were included. The review identified 24 studies from 16 countries meeting the inclusion criteria, with an overall sample size of more than 860,000 children and adolescents. Through a standardized quality assessment tool, we classified two studies as strong, 21 as moderate, and only one as weak. We analyzed specific secular trends separately for the five different dimensions: endurance, strength, speed, flexibility, and coordination. The majority of studies report a decline of PF over time; however, a few studies report conflicting results. Performance in endurance, strength, and flexibility decreased over time, whereas there was no consistent trend reported for speed and coordination. Overall, there is no international standard on examining and reporting changes or secular trends in PF of children and adolescents, and comparability of studies is limited due to heterogeneous conditions of conducting and analyzing PF tests. Consequently, standardized and consistent international monitoring should be implemented.
Extracellular ATP as an Inter-Kingdom Signaling Molecule: Release Mechanisms by Bacteria and Its Implication on the Host
Authors: Spari Daniel , Beldi Guido
Published: August 2020

The purine adenosine 5′-triphosphate (ATP) is not only a universal intracellular energy carrier but plays also an important role as extracellular signaling molecule. Purinergic signaling is involved in many physiological and pathological processes like coagulation, inflammation, or sepsis in mammals. ATP is well-known as a messenger for intercellular communications in multicellular organisms, but phylogenetically much older unicellular organisms like yeast or bacteria use ATP as an extracellular signaling molecule as well. However, the mechanisms of ATP secretion by bacteria and its extracellular implications still have to be elucidated. This review will provide an overview of the current knowledge about bacterial extracellular ATP (eATP) under homeostatic conditions and during growth. Possible secretion mechanisms of ATP by bacteria will be discussed and implications of bacterial ATP are shown, with a focus on bacteria–host interactions.
Upregulation of the TRPA1 Ion Channel in the Gastric Mucosa after Iodoacetamide-Induced Gastritis in Rats: A Potential New Therapeutic Target
Authors: Csekő Kata , Pécsi Dániel , Kajtár Béla , Hegedűs Ivett , Bollenbach Alexander , Tsikas Dimitrios , Szabó Imre László , Szabó Sándor , Helyes Zsuzsanna
Published: August 2020

Acute gastritis is often untreatable by acid secretion-inhibiting drugs. Understanding the protective mechanisms including the role of Transient Receptor Potential Ankyrin1 (TRPA1) and Vanilloid1 (TRPV1) channels localized on capsaicin-sensitive afferents and non-neuronal structures might identify novel therapeutic approaches. Therefore, we characterized a translational gastritis model using iodoacetamide (IAA) and investigated TRPA1/V1 expressions. Wistar rats and CD1, C57Bl/6J mice were exposed to IAA-containing (0.05, 0.1, 0.2, 0.3, 0.5%) drinking water for 7 or 14 days. Body weight and water consumption were recorded daily. Macroscopic lesions were scored, qualitative histopathologic investigation was performed, TRPA1/V1 immunopositivity and mRNA expressions were measured. IAA induced a concentration-dependent weight loss and reduced water intake in both species. Hyperemia, submucosal edema, inflammatory infiltration and hemorrhagic erosions developed after 7 days, while ulcers after 14 days in rats. Trpa1 mRNA/protein expressions were upregulated at both timepoints. Meanwhile, TRPV1 immunopositivity was upregulated in the gastric corpus after 0.05% IAA ingestion, but downregulated after 0.2%, whereas Trpv1 mRNA did not change. Interestingly, no macroscopic/microscopic changes were observed in mice. These are the first data for the concentration- and duration-dependent changes in the IAA-induced gastritis in rats accompanied by TRPA1 upregulation, therefore, its therapeutic potential in gastritis should further be investigated.
The Specific Molecular Composition and Structural Arrangement of Eleutherodactylus Coqui Gular Skin Tissue Provide Its High Mechanical Compliance
Authors: Hui Justin , Sharma Shivang , Rajani Sarah , Singh Anirudha
Published: August 2020

A male Eleutherodactylus Coqui (EC, a frog) expands and contracts its gular skin to a great extent during mating calls, displaying its extraordinarily compliant organ. There are striking similarities between frog gular skin and the human bladder as both organs expand and contract significantly. While the high extensibility of the urinary bladder is attributed to the unique helical ultrastructure of collagen type III, the mechanism behind the gular skin of EC is unknown. We therefore aim to understand the structure–property relationship of gular skin tissues of EC. Our findings demonstrate that the male EC gular tissue can elongate up to 400%, with an ultimate tensile strength (UTS) of 1.7 MPa. Species without vocal sacs, Xenopus Laevis (XL) and Xenopus Muelleri (XM), elongate only up to 80% and 350% with UTS~6.3 MPa and ~4.5 MPa, respectively. Transmission electron microscopy (TEM) and histological staining further show that EC tissues’ collagen fibers exhibit a layer-by-layer arrangement with an uninterrupted, knot-free, and continuous structure. The collagen bundles alternate between a circular and longitudinal shape, suggesting an out-of-plane zig-zag structure, which likely provides the tissue with greater extensibility. In contrast, control species contain a nearly linear collagen structure interrupted by thicker muscle bundles and mucous glands. Meanwhile, in the rat bladder, the collagen is arranged in a helical structure. The bladder-like high extensibility of EC gular skin tissue arises despite it having eight-fold lesser elastin and five times more collagen than the rat bladder. To our knowledge, this is the first study to report the structural and molecular mechanisms behind the high compliance of EC gular skin. We believe that these findings can lead us to develop more compliant biomaterials for applications in regenerative medicine.
The Significance of Mitochondrial Dysfunction in Cancer
Authors: Luo Yongde , Ma Jianjia , Lu Weiqin
Published: August 2020

As an essential organelle in nucleated eukaryotic cells, mitochondria play a central role in energy metabolism, maintenance of redox balance, and regulation of apoptosis. Mitochondrial dysfunction, either due to the TCA cycle enzyme defects, mitochondrial DNA genetic mutations, defective mitochondrial electron transport chain, oxidative stress, or aberrant oncogene and tumor suppressor signaling, has been observed in a wide spectrum of human cancers. In this review, we summarize mitochondrial dysfunction induced by these alterations that promote human cancers.
A Plant-Derived Antigen–Antibody Complex Induces Anti-Cancer Immune Responses by Forming a Large Quaternary Structure
Authors: Kim Deuk-Su , Kang Yang Joo , Lee Kyung Jin , Qiao Lu , Ko Kinarm , Kim Dae Heon , Myeung Soon Chul , Ko Kisung
Published: August 2020

The antigen–antibody complex (AAC) has novel functions for immunomodulation, encouraging the application of diverse quaternary protein structures for vaccination. In this study, GA733 antigen and anti-GA733 antibody proteins were both co-expressed to obtain the AAC protein structures in a F1 plant obtained by crossing the plants expressing each protein. In F1 plant, the antigen and antibody assembled to form a large quaternary circular ACC structure (~30 nm). The large quaternary protein structures induced immune response to produce anticancer immunoglobulins G (IgGs) that are specific to the corresponding antigens in mouse. The serum containing the anticancer IgGs inhibited the human colorectal cancer cell growth in the xenograft nude mouse. Taken together, antigens and antibodies can be assembled to form AAC protein structures in plants. Plant crossing represents an alternative strategy for the formation of AAC vaccines that efficiently increases anticancer antibody production.
Murine Long Noncoding RNA Morrbid Contributes in the Regulation of NRAS Splicing in Hepatocytes In Vitro
Authors: Fefilova Anna , Melnikov Pavel , Prikazchikova Tatiana , Abakumova Tatiana , Kurochkin Ilya , Mazin Pavel V. , Ziganshin Rustam , Sergeeva Olga , Zatsepin Timofei S.
Published: August 2020

The coupling of alternative splicing with the nonsense-mediated decay (NMD) pathway maintains quality control of the transcriptome in eukaryotes by eliminating transcripts with premature termination codons (PTC) and fine-tunes gene expression. Long noncoding RNA (lncRNA) can regulate multiple cellular processes, including alternative splicing. Previously, murine Morrbid (myeloid RNA repressor of Bcl2l11 induced death) lncRNA was described as a locus-specific controller of the lifespan of short-living myeloid cells via transcription regulation of the apoptosis-related Bcl2l11 protein. Here, we report that murine Morrbid lncRNA in hepatocytes participates in the regulation of proto-oncogene NRAS (neuroblastoma RAS viral oncogene homolog) splicing, including the formation of the isoform with PTC. We observed a significant increase of the NRAS isoform with PTC in hepatocytes with depleted Morrbid lncRNA. We demonstrated that the NRAS isoform with PTC is degraded via the NMD pathway. This transcript is presented almost only in the nucleus and has a half-life ~four times lower than other NRAS transcripts. Additionally, in UPF1 knockdown hepatocytes (the key NMD factor), we observed a significant increase of the NRAS isoform with PTC. By a modified capture hybridization (CHART) analysis of the protein targets, we uncovered interactions of Morrbid lncRNA with the SFPQ (splicing factor proline and glutamine rich)-NONO (non-POU domain-containing octamer-binding protein) splicing complex. Finally, we propose the regulation mechanism of NRAS splicing in murine hepatocytes by alternative splicing coupled with the NMD pathway with the input of Morrbid lncRNA.
An Inducible Diabetes Mellitus Murine Model Based on MafB Conditional Knockout under MafA-Deficient Condition
Authors: Deng Zhaobin , Matsumoto Yuka , Kuno Akihiro , Ojima Masami , Xiafukaiti Gulibaikelamu , Takahashi Satoru
Published: August 2020

Diabetes mellitus is an increasingly severe chronic metabolic disease that is occurring at an alarming rate worldwide. Various diabetic models, including non-obese diabetic mice and chemically induced diabetic models, are used to characterize and explore the mechanism of the disease’s pathophysiology, in hopes of detecting and identifying novel potential therapeutic targets. However, this is accompanied by disadvantages, such as specific conditions for maintaining the incidence, nonstable hyperglycemia induction, and potential toxicity to other organs. Murine MAFA and MAFB, two closely-linked islet-enriched transcription factors, play fundamental roles in glucose sensing and insulin secretion, and maintenance of pancreatic β-cell, respectively, which are highly homologous to human protein orthologs. Herein, to induce the diabetes mellitus model at a specific time point, we generated Pdx1-dependent Mafb-deletion mice under Mafa knockout condition (A0BΔpanc), via tamoxifen-inducible Cre-loxP system. After 16 weeks, metabolic phenotypes were characterized by intraperitoneal glucose tolerance test (IPGTT), urine glucose test, and metabolic parameters analysis. The results indicated that male A0BΔpanc mice had obvious impaired glucose tolerance, and high urine glucose level. Furthermore, obvious renal lesions, impaired islet structure and decreased proportion of insulin positive cells were observed. Collectively, our results indicate that A0BΔpanc mice can be an efficient inducible model for diabetes research.
Dietary Protein and Fat Intake Affects Diabetes Risk with CDKAL1 Genetic Variants in Korean Adults
Authors: Choi Woo Jeong , Jin Hyun-Seok , Kim Sung-Soo , Shin Dayeon
Published: August 2020

Cyclin-dependent kinase 5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is one of the strongest diabetes loci identified to date; evidence suggests that it plays an important role in insulin secretion. Dietary factors that affect insulin demand might enhance the risk of diabetes associated with CDKAL1 variants. Our aim was to examine the interactions between dietary protein and fat intake and CDKAL1 genetic variants in relation to the risk of diabetes in Korean adults. Single nucleotide polymorphisms (SNPs) were selected with a genome-wide association study (GWAS) for diabetes after adjustment for age, gender, and examination site. Using data from the Health Examinees (HEXA) Study of the Korean Genome and Epidemiology Study (KoGES), 3988 middle-aged Korean adults between 40–76 years of age (2034 men and 1954 women) were included in the study. Finally, rs7756992 located within the CDKAL1 gene region was selected from GWAS (p-value < 5 × 10−8). Multivariable logistic regression models were used to evaluate the interactions between genotypes and dietary protein and fat intake in relation to diabetes risk after adjustment for age, gender, BMI, waist circumference, physical activity, smoking status, drinking habits, and examination site. Significant interactions between CDKAL1 rs7756992 and dietary protein and fat intake for the risk of diabetes were observed in men (p-value < 0.05). In women, significant interactions between dietary protein and fat intake and CDKAL1 variants (rs7756992) were associated with increased risk of diabetes (p-value < 0.05). Dietary protein and fat intake interacted differently with CDKAL1 variants in relation to the risk of diabetes in Korean adults of both genders. These findings indicate that CDKAL1 variants play a significant role in diabetes and that dietary protein and fat intake could affect these associations.
Discovery of Novel Agents on Spindle Assembly Checkpoint to Sensitize Vinorelbine-Induced Mitotic Cell Death against Human Non-Small Cell Lung Cancers
Authors: Chang Ya-Ching , Tseng Yu-Ling , Leu Wohn-Jenn , Du Chi-Min , Jiang Yi-Huei , Hsu Lih-Ching , Hsu Jui-Ling , Hou Duen-Ren , Guh Jih-Hwa
Published: August 2020

Non-small cell lung cancer (NSCLC) accounts about 80% of all lung cancers. More than two-thirds of NSCLC patients have inoperable, locally advanced or metastatic tumors. Non-toxic agents that synergistically potentiate cancer-killing activities of chemotherapeutic drugs are in high demand. YL-9 was a novel and non-cytotoxic compound with the structure related to sildenafil but showing much less activity against phosphodiesterase type 5 (PDE5). NCI-H460, an NSCLC cell line with low PDE5 expression, was used as the cell model. YL-9 synergistically potentiated vinorelbine-induced anti-proliferative and apoptotic effects in NCI-H460 cells. Vinorelbine induced tubulin acetylation and Bub1-related kinase (BUBR1) phosphorylation, a necessary component in spindle assembly checkpoint. These effects, as well as BUBR1 cleavage, were substantially enhanced in co-treatment with YL-9. Several mitotic arrest signals were enhanced under combinatory treatment of vinorelbine and YL-9, including an increase of mitotic spindle abnormalities, increased cyclin B1 expression, B-cell lymphoma 2 (Bcl-2) phosphorylation and increased phosphoproteins. Moreover, YL-9 also displayed synergistic activity in combining with vinorelbine to induce apoptosis in A549 cells which express PDE5. In conclusion. the data suggest that YL-9 is a novel agent that synergistically amplifies vinorelbine-induced NSCLC apoptosis through activation of spindle assembly checkpoint and increased mitotic arrest of the cell cycle. YL-9 shows the potential for further development in combinatory treatment against NSCLC.
Spectroscopic Investigation of the Kinetic Mechanism Involved in the Association of Potyviral VPg with the Host Plant Translation Initiation Factor eIF4E
Authors: Walter Jocelyne , Barra Amandine , Charon Justine , Tavert-Roudet Geneviève , Michon Thierry
Published: August 2020

The infectious cycle of potyviruses requires the formation of a complex between the viral genome-linked protein VPg and the host eukaryotic translation initiation factor 4E, eIF4E. Mutations associated with plant resistance to potyviruses were previously mapped at the eIF4E surface, while on the virus side, mutations leading to plant resistance breaking were identified within the VPg. In the present study, fluorescence spectroscopy was used to probe the contribution of the VPg intrinsically disordered region bearing amino acids determinant of the resistance breaking, to the VPg–eIF4E binding mechanism. Synthetic peptides encompassing the VPg88–120 central region were found to tightly bind to eIF4E. Fluorescence energy transfer experiments show that, upon binding to eIF4E, the N and C termini of the VPg88–111 fragment move closer to one another, at a distance compatible with a α-helix folding. When the VPg112–120 region, which contains amino acids associated with resistance breakdown, is appended to VPg88–111, the complex formation with eIF4E switches from a single-step to a two-step kinetic model. This study revisits a recent investigation of the VPg–eIF4E complex by specifying the contribution of the VPg central helix and its appended disordered region to VPg association with eIF4E.
Multidrug-Resistant Gram-Negative Bacteria Decolonization in Immunocompromised Patients: A Focus on Fecal Microbiota Transplantation
Authors: Alagna Laura , Palomba Emanuele , Mangioni Davide , Bozzi Giorgio , Lombardi Andrea , Ungaro Riccardo , Castelli Valeria , Prati Daniele , Vecchi Maurizio , Muscatello Antonio , Bandera Alessandra , Gori Andrea
Published: August 2020

Antimicrobial resistance is an important issue for global health; in immunocompromised patients, such as solid organ and hematological transplant recipients, it poses an even bigger threat. Colonization by multidrug-resistant (MDR) bacteria was acknowledged as a strong risk factor to subsequent infections, especially in individuals with a compromised immune system. A growing pile of studies has linked the imbalance caused by the dominance of certain taxa populating the gut, also known as intestinal microbiota dysbiosis, to an increased risk of MDR bacteria colonization. Several attempts were proposed to modulate the gut microbiota. Particularly, fecal microbiota transplantation (FMT) was successfully applied to treat conditions like Clostridioides difficile infection and other diseases linked to gut microbiota dysbiosis. In this review we aimed to provide a look at the data gathered so far on FMT, focusing on its possible role in treating MDR colonization in the setting of immunocompromised patients and analyzing its efficacy and safety.
The Importance of Males to Bumble Bee (Bombus Species) Nest Development and Colony Viability
Authors: Belsky Joseph E. , Camp Allison A. , Lehmann David M.
Published: August 2020

Bumble bee population declines over the last decade have stimulated strong interest in determining causative factors and necessary conservation measures. Research attention has largely been directed toward bumble bee worker and queen health and their contributions to population stability, while male bees (i.e., drones) have typically been overlooked regarding their role in influencing colony fitness and longevity. In this review we assess existing literature on the diverse role of males within bumble bee nests and their importance to queen health and fitness, as well as to overall nest success. The implications of reproductive measures, including sperm transfer, mating behavior, mating plugs, and male immunity, among other topics, are examined. Overall, bumble bee males are found to drive colony function in a unique manner. Current knowledge gaps pertaining to the role of males are discussed. We highlight the importance of drones to queen success and fitness in many ways, and suggest future research exploring impacts of this often-neglected caste.
Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra® 3D) on the Foraging and Blood-Feeding Behaviors of Aedes albopictus Using Laboratory Rodent Model
Authors: Laidoudi Younes , Tahir Djamel , Medkour Hacène , Varloud Marie , Mediannikov Oleg , Davoust Bernard
Published: August 2020

Dinotefuran-Permethrin-Pyriproxyfen (DPP) is used to kill and repel mosquitoes from dogs. However, the influence of the product on the host-seeking behavior of mosquitoes remains unknown. The interference of DPP with the host selection of unfed female Aedes albopictus was investigated. A total of 18 animals (9 mice and 9 rats) were divided into three groups of six animals each. DU: DPP treated rats (n = 3) with untreated mice (n = 3), UD: DPP treated mice (n = 3) with untreated rats (n = 3) and control UU: untreated mice (n = 3) and untreated rats (n = 3). In each group, the rats and mice were placed 30 cm apart. After sedation, the animals in each group were exposed twice (Day 1 and Day 7 post-treatment) for one hour to 71 ± 3 female mosquitoes. Mosquitoes were categorized after the 2-h post-exposure period as dead or alive. Blood-meal origin was determined from mosquitoes using a newly customized duplex qPCR. The highest values of forage ratio (1.36 ≥ wi ≤ 1.88) and selection index (0.63 ≥ Bi ≤ 0.94) for rat hosts indicates a preference of mosquitoes for this species as compared to mice when co-housed during the exposure. The mosquitoes only seldom fed on mice, even in the untreated group. The anti-feeding effect of DPP was therefore only assessed on rat’s hosts. The results showed that DPP, when directly applied on rats, provided a direct protection of 82% and 61% on Day 1 and Day 7, respectively, while when applied on mice hosts (UD), the DPP provided an indirect protection of 21% and 10% on Day 1 and Day 7, respectively. The results showed also that DPP, when applied on rats, provided a direct protection against Ae. albopictus bites. This effect did not result in increased exposure of the untreated host placed in the same cage at a distance of 30 cm.
Essential Oils as Post-Harvest Crop Protectants against the Fruit Fly Drosophila suzukii: Bioactivity and Organoleptic Profile
Authors: Bedini Stefano , Cosci Francesca , Tani Camilla , Pierattini Erika Carla , Venturi Francesca , Lucchi Andrea , Ioriatti Claudio , Ascrizzi Roberta , Flamini Guido , Ferroni Giuseppe , Taglieri Isabella , Conti Barbara
Published: August 2020

The essential oils extracted from mandarin (Citrus reticulata Blanco) fruits, and from tea tree (Maleleuca alternifolia (Maiden and Betche) Cheel) leaves have been chemically analyzed and tested for their bioactivity against D. suzukii. Besides, to estimate consumers’ acceptability of the essential oil (EO) treatments, we evaluated their impact on the organoleptic characteristics of the EO-treated fruits. The main chemical constituents of the two EOs were 1,8-cineole and 4-terpineol for M. alternifolia (22.4% and 17.6% of the total components, respectively), and limonene (83.6% of the total components) for C. reticulata. The behavioral tests indicate that the two EOs are able to deter D. suzukii oviposition and that D. suzukii shows positive chemotaxis to low concentrations of the EOs and negative chemotaxis when the EO concentration increases. While no negative effects on the organoleptic profiles were detected for fruits treated with C. reticulata EO, the olfactory profile of fruits treated with M. alternifolia EO was so negative that they were defined as “not suitable for consumption” by panellists. Overall, our findings indicate that the use of EOs for the post-harvest protection of small fruits is feasible, provided that the essential oils are selected not only for their bioactivity against the insect pest but also for their affinity with the consumers’ sensorial system.
Characterization of Raman Spectroscopy System Transfer Functions in Intensity, Wavelength, and Time
Authors: Lin Yu-Chung , Sinfield Joseph V.
Published: August 2020

The emergence of a wide variety of relatively low-cost compact spectrometers has led to an increase in the use of spectroscopic techniques by researchers in a broad array of fields beyond those that have traditionally employed these analytical methods. While the fundamental elements and functions of Raman systems are generally consistent, the specific components that compose a system may vary in number, design, and configuration, and researchers often modify off-the-shelf spectrometers for unique applications. Understanding the effect of instrument design and components on acquired information is thus crucial and provides the prospect to optimize the system to individual needs and to properly compare results obtained with different systems while also reducing the potential for unintended misinterpretation of data. This paper provides a practical treatment of the influences in a typical compact spectroscopy system that can impact the extent to which the output of the system is representative of the observed environment, a relationship that in measurement science is classically termed the system transfer function. For clarity, the transfer function is developed in terms of traditional Raman output parameters, namely intensity, wavelength, and time.
The Association Between Hypertension in Pregnancy and Preterm Birth with Fetal Growth Restriction in Singleton and Twin Pregnancy: Use of Twin Versus Singleton Charts
Authors: Kalafat Erkan , Abiola Aisha , Thilaganathan Basky , Bhide Amar , Khalil Asma
Published: August 2020

Objective: To compare the rates of fetal growth restriction (FGR) in singleton and twin pregnancies using singleton and twin-specific birthweight standards. Methods: The study included liveborn twin and singleton pregnancies between January 2000 and January 2019. Hypertensive disorders of pregnancy (HDP) included gestational hypertension and pre-eclampsia. The study outcomes were FGR or small-for-gestational-age (SGA) at birth as assessed using singleton and twin reference charts. Results: The analysis included 1473 twin and 62,432 singleton pregnancies. In singleton pregnancies the risk of PTB <34 weeks without HDP (OR 2.82, p < 0.001), delivery ≥34 weeks with HDP (OR 2.38, p < 0.001), and PTB <34 weeks with HDP (OR 13.65, p < 0.001) were significantly higher in the pregnancies complicated by FGR compared to those without. When selective fetal growth restriction (sFGR) was assessed using the singleton standard, the risk of PTB <34 weeks without HDP (OR 1.03, p = 0.872), delivery ≥34 weeks with HDP (OR 1.36, p = 0.160) were similar in the pregnancies complicated by sFGR compared to those without, while the risk of PTB <34 weeks with HDP (OR 2.41, p = 0.025) was significantly higher in the pregnancies complicated by sFGR compared to those without. When sFGR was assessed using the twin-specific chart, the risk of PTB <34 weeks without HDP (OR 3.55, p < 0.001), delivery ≥34 weeks with HDP (OR 3.17, p = 0.004), and PTB <34 weeks with HDP (OR 5.69, p < 0.001) were significantly higher in the pregnancies complicated by sFGR compared to those without. The stronger and more consistent association persisted in the subgroup analyses according to chorionicity. The strength of association in dichorionic twin pregnancies resembles that of the singletons more closely and consistently when the FGR was diagnosed using the twin-specific charts. Conclusion: FGR in twin pregnancies has a stronger and more consistent association with HDP and PTB when using twin-specific rather than singleton charts. This study provides further evidence supporting the use of twin-specific charts when assessing fetal growth in twin pregnancies.
Environmental Impact on Male (In)Fertility via Epigenetic Route
Authors: Cescon Matilde , Chianese Rosanna , Tavares Renata S.
Published: August 2020

In the last 40 years, male reproductive health—which is very sensitive to both environmental exposure and metabolic status—has deteriorated and the poor sperm quality observed has been suggested to affect offspring development and its health in adult life. In this scenario, evidence now suggests that epigenetics shapes endocrine functions, linking genetics and environment. During fertilization, spermatozoa share with the oocyte their epigenome, along with their haploid genome, in order to orchestrate embryo development. The epigenetic signature of spermatozoa is the result of a dynamic modulation of the epigenetic marks occurring, firstly, in the testis—during germ cell progression—then, along the epididymis, where spermatozoa still receive molecules, conveyed by epididymosomes. Paternal lifestyle, including nutrition and exposure to hazardous substances, alters the phenotype of the next generations, through the remodeling of a sperm epigenetic blueprint that dynamically reacts to a wide range of environmental and lifestyle stressors. With that in mind, this review will summarize and discuss insights into germline epigenetic plasticity caused by environmental stimuli and diet and how spermatozoa may be carriers of induced epimutations across generations through a mechanism known as paternal transgenerational epigenetic inheritance.
Mental Health of Medical and Non-Medical Professionals during the Peak of the COVID-19 Pandemic: A Cross-Sectional Nationwide Study
Authors: Maciaszek Julian , Ciulkowicz Marta , Misiak Blazej , Szczesniak Dorota , Luc Dorota , Wieczorek Tomasz , Fila-Witecka Karolina , Gawlowski Pawel , Rymaszewska Joanna
Published: August 2020

Background: The study aimed to compare psychopathological expressions during the COVID-19 (novel coronavirus disease 2019) pandemic, as declared on March 11th 2020 by the World Health Organization, with respect to which institutional variables might distinguish the impact of COVID-19 in medical and non-medical professionals. Methods: A cross-sectional study was performed nationwide between 16th March and the 26th April 2020 in Poland. A total of 2039 respondents representing all healthcare providers (59.8%) as well as other professionals filled in the sociodemographic section, the General Health Questionnaire-28 and the author’s questionnaire with questions related to exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the availability of protective measures, quarantine, change of working hours and place of employment during the pandemic, as well as feelings associated with the state of the pandemic. Results: Medical professionals more often presented with relevant psychopathological symptoms (GHQ-28 (General Health Questionnaire-28) total score >24) than the non-medical group (60.8% vs. 48.0%, respectively) such as anxiety, insomnia and somatic symptoms even after adjustment for potential confounding factors. Male sex, older age and appropriate protective equipment were associated with significantly lower GHQ-28 total scores in medical professionals, whereas among non-medical professionals, male sex was associated with significantly lower GHQ-28 total scores. Conclusions: Somatic and anxiety symptoms as well as insomnia are more prevalent among medical staff than workers in other professions. Targeting the determinants of these differences should be included in interventions aimed at restoring psychological well-being in this specific population. Apparently, there are present gender differences in psychological responses that are independent of profession.
Associations between Subsequent Hospitalizations and Primary Ambulatory Services Utilization within the First Year after Acute Myocardial Infarction and Long-Term Mortality
Authors: Plakht Ygal , Greenberg Dan , Gilutz Harel , Arbelle Jonathan Eli , Shiyovich Arthur
Published: August 2020

Healthcare resource utilization peaks throughout the first year following acute myocardial infarction (AMI). Data linking the former and outcomes are sparse. We evaluated the associations between subsequent length of in-hospital stay (SLOS) and primary ambulatory visits (PAV) within the first year after AMI and long-term mortality. This retrospective analysis included patients who were discharged following an AMI. Study groups: low (0–1 days), intermediate (2–7) and high (≥8 days) SLOS; low (<10) and high (≥10 visits) PAV, throughout the first post-AMI year. All-cause mortality was set as the primary outcome. Overall, 8112 patients were included: 55.2%, 23.4% and 21.4% in low, intermediate and high SLOS groups respectively; 26.0% and 74.0% in low and high-PAV groups. Throughout the follow-up period (up to 18 years), 49.6% patients died. Multivariable analysis showed that an increased SLOS (Hazard ratio (HR) = 1.313 and HR = 1.714 for intermediate and high vs. low groups respectively) and a reduced number of PAV (HR = 1.24 for low vs. high groups) were independently associated with an increased risk for mortality (p < 0.001 for each). Long-term mortality following AMI is associated with high hospital and low primary ambulatory services utilization throughout the first-year post-discharge. Measures focusing on patients with increased SLOS and reduced PAV should be considered to improve patient outcomes.
Thermal Analysis of Crosslinking Reactions in Epoxy Nanocomposites Containing Polyvinyl Chloride (PVC)-Functionalized Nickel-Doped Nano-Fe3O4
Authors: Jouyandeh Maryam , Ganjali Mohammad Reza , Karami Zohre , Rezapour Morteza , Bagheri Babak , Zarrintaj Payam , Mouradzadegun Arash , Habibzadeh Sajjad , Saeb Mohammad Reza
Published: August 2020

This work reports on the thermal analysis of epoxy containing polyvinyl chloride (PVC) surface-functionalized magnetic nanoparticles (PVC–S/MNP) and its bulk-modified nickel-doped counterpart (PVC–S/MNP/Bi–B). Nanoparticles were synthesized through the cathodic electro-deposition method. The morphology of particles was imaged on a field-emission scanning electron microscope (FE-SEM), while X-ray diffraction analysis and Fourier-transform infrared spectroscopy (FTIR) were used to detect changes in the structure of nanoparticles. The magnetic behavior of particles was also studied by vibrating sample magnetometry (VSM). In particular, we focused on the effect of the bulk (Ni-doping) and surface (PVC-capping) modifications of MNPs on the thermal crosslinking of epoxy using nonisothermal differential scanning calorimetry (DSC) varying the heating rate. The cure labels of the prepared nanocomposites were assigned to them, as quantified by the cure index. The good cure state was assigned to the system containing PVC–S/MNP/Bi–B as a result of excessive ring opening of epoxy. Cure kinetics parameters of PVC–S/MNP/Bi–B incorporated epoxy was obtained by the use of isoconversional methodology. The activation energy of epoxy was decreased upon addition of 0.1 wt% of PVC–S/MNP/Bi–B due to the reaction of Cl− of PVC by the functional groups of resin.