bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Gibberellic Acid to Increase the Salt Tolerance of Leaf Lettuce and Rocket Grown in a Floating System
Authors: Vetrano Filippo , Moncada Alessandra , Miceli Alessandro
Published: April 2020

Hydroponics need water of good quality to prepare a balanced nutrient solution that could allow plants to reach their maximum yield potential. The rising difficulties in finding water with good quality have led to the compelling necessity of identifying sustainable ways to use saline water, limiting its negative effect on crop yield and quality. The exogenous supplementation of plant growth regulators, such as gibberellic acid (GA3), can be effective in increasing plant growth and vigor, thus helping plants to better cope with salt stress. The aim of this study was to evaluate the feasibility to increase the salt tolerance of leaf lettuce and rocket grown in a floating system by adding GA3 (10−6 M) to mineral nutrient solutions (MNS) with increasing salinity (0, 10, and 20 mM NaCl). Leaf lettuce and rocket plants suffered a significant reduction of growth and yield, determined by the reduction of biomass, leaf number, and leaf area, even with moderate salt stress (10 mM NaCl). The supplementation of exogenous GA3 through the MNS allowed plants to substantially counterbalance salt stress by enhancing various morphological and physiological traits, such as biomass accumulation, leaf expansion, stomatal conductance and water and nitrogen use efficiency. The effects of salt stress and GA3 treatment varied according to the species, thus indicating that this interaction may improve salt tolerance by activating different adaptation systems.
Barriers and Facilitators for Adopting Sustainable Soil Management Practices in Mediterranean Olive Groves
Authors: Aznar-Sánchez José A. , Velasco-Muñoz Juan F. , López-Felices Belén , del Moral-Torres Fernando
Published: April 2020

Soil is a fundamental resource, subject to severe and quick degradation processes because of the pressure of human activities, particularly in many regions of the Mediterranean where agriculture is an important economic activity. It has been proven that the use of sustainable soil management practices can potentially give rise to the creation of a carbon sink, an increase of soil organic matter content, the maintenance of crop productivity and a reduction in erosion. Despite the existence of scientific evidence about the benefits generated by the use of sustainable practices on soil, many farmers are reluctant to adopt them. The objective of this study is to identify and give a hierarchical structure to the factors that condition the adoption of sustainable practices in the management of agricultural soil. The case of olive tree cultivation in Southeast Spain has been studied, using a participatory qualitative methodology. The results show a series of seven principal barriers (information, costs, risk aversion, characteristics of the farm and sustainable practices, macro factors, and cultural barriers) and five facilitators (technology, farmer training, awareness, incentives, and social pressure) for the adoption of the proposed sustainable agricultural practices. The principal political and legislative actions proposed to increase the adoption of sustainable agricultural practices include: administrative control, fostering environmental awareness, technical knowledge, and on-farm demonstrations; and, on the economic and financial level, incorporation of both general incentives and subsidizing specific costs. This study contributes to the development and discussion of intervention proposals that are designed to stimulate the implementation of sustainable practices in agricultural soil management.
Soil Carbon Dynamics under Pastures in Andean Socio-Ecosystems of Colombia
Authors: Ordoñez María-Cristina , Casanova Olaya Juan Fernando , Galicia Leopoldo , Figueroa Apolinar
Published: April 2020

Models can help to explain the main interactions, magnitudes, and velocity by which biological processes accumulate soil organic carbon (SOC) in pastures. An explanatory model using Insight Maker software was constructed for each soil under natural and cultivated pastures, using theoretical carbon models and data which were collected monthly in andisol sites. The model was calibrated and validated by comparing the modeled data to the field data until the smallest prediction error was reached. The indicators used were the mean absolute error (MAE), root-mean-square error (RMSE), mean absolute percentage error (MAPE) and the coefficient of determination (R2). In natural pasture soil, the diversification of organic inputs consistently promoted the growth of microbial biomass and metabolic efficiency. In contrast, intensive management of cultivated pastures, involving the removal of plant cover, plowing and low input of organic matter, stressed the microbial community and increased the potential carbon loss through secondary mineralization and surface runoff. The application of modeling indicated that it is necessary to improve agronomic practices in cultivated pastures, to maintain soil cover and to increase the application of organic fertilizer by 1.5 times, in order to reduce stress on the microbial biomass, accumulate SOC, minimize organic matter mineralization and reduce C losses due to surface runoff. Therefore, improving agricultural management based on the understanding of soil processes will allow increasing the potential for SOC storage, while improving pasture sustainability.
Productivity, Energy and Economic Balance in the Production of Different Cultivars of Winter Oilseed Rape. A Case Study in North-Eastern Poland
Authors: Sokólski Mateusz , Jankowski Krzysztof Józef , Załuski Dariusz , Szatkowski Artur
Published: April 2020

In this study, the agricultural inputs, energy requirements and costs associated with the production of semi-dwarf (PR45 D03 and Avenir) and long-stem (Visby) cultivars of winter oilseed rape were optimized in an experiment with 35-1 fractional factorial design. A field experiment was carried out in the Agricultural Experiment Station in Bałcyny (north-eastern Poland) in 2008–2011. The study investigated the responses of two morphotypes of hybrid cultivars of winter oilseed rape to key yield-forming factors (seeding date, seeding rate, nitrogen fertilization) and yield protection factors (fungal disease control). Agronomic inputs were tested at three levels. Our findings indicate that production technologies (characterized by a different intensity of agricultural inputs) should target the specific requirements of winter oilseed rape cultivars. Semi-dwarf cultivars of winter oilseed rape (PR45 D03 and Avenir) were characterized by higher yield potential at different input levels than the long-stem cultivar (Visby). Semi-dwarf cultivars required higher levels of agricultural inputs than the long-stem cultivar. Semi-dwarf cultivars grown in high-input technologies were characterized by the highest energy efficiency ratio. In contrast, the long-stem cultivar was characterized by the optimal energy input-energy output ratio in the low-input technology. Regardless of cultivar, high-input production technologies were more profitable because the resulting increase in seed yield significantly outweighed the rise in production costs.
The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)
Authors: Petrović Tamara G. , Vučić Tijana Z. , Nikolić Sonja Z. , Gavrić Jelena P. , Despotović Svetlana G. , Gavrilović Branka R. , Radovanović Tijana B. , Faggio Caterina , Prokić Marko D.
Published: April 2020

Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge activates some stress response, its effect on oxidative stress has not been adequately examined. This study investigated whether the presence/absence of a shelter modifies the oxidative status (the antioxidant system and oxidative damage) and aggressive behavior of crested newt larvae (Triturus macedonicus and its hybrid with T. ivanbureschi). Our results show that individuals reared with shelters had lower values of the tested antioxidant parameters (catalase, glutathione peroxidase, glutathione S-transferase and glutathione), indicating a lower production of reactive species than individuals reared without shelter. The same pattern was observed in both T. macedonicus and its hybrid. Contrary to the activation of some physiological pathways, shelter availability did not significantly affect the rate of intraspecific aggressive behavior. The physiological benefits of shelter use can be manifested as a lower requirement for investment in the energy necessary for the maintenance of the upregulated antioxidant defenses, activation of repair systems and synthesis of endogenous antioxidants. This study highlights the importance of shelter provision, which may be valuable in habitat restoration and animal conservation studies.
Feather Corticosterone Measurements of Greater Flamingos Living under Different Forms of Flight Restraint
Authors: Reese Lukas , Baumgartner Katrin , von Fersen Lorenzo , Merle Roswitha , Ladwig-Wiegard Mechthild , Will Hermann , Haase Gudrun , Tallo-Parra Oriol , Carbajal Annaïs , Lopez-Bejar Manel , Thöne-Reineke Christa
Published: April 2020

Deflighting zoo birds is a practice that receives increasing criticism due to its presumed incompatibility with animal welfare. To our knowledge, this is the first approach to address this problem in a scientific way. To do this, we compared feather corticosterone (CORTf) from Greater Flamingos (Phoenicopterus roseus, n = 151) of different flight status (i.e., pinioned, feather clipped, airworthy) from twelve different zoological institutions. To complement the hormone measurements, behavioral observations (scan samplings) were conducted prior to feather sampling within the presumed time frame of feather growth. We hypothesized that CORTf of the deflighted flamingos would differ from CORTf of their airworthy conspecifics. No significant difference in CORTf was found between the three groups, and our hypothesis was rejected. However, the impact of the institution itself (i.e., the housing conditions) proved to be the most dominant variable (variance between the institutions = 53.82%). Due to high variability, the behavioral observations were evaluated descriptively but did not give rise to doubt the findings in CORTf. Therefore, we assume that the method of flight restraint of Greater Flamingos does not have a measurable effect on CORTf. We consider this model for evaluating animal welfare of zoo birds a useful tool and provide ideas for further adjustments for consecutive studies.
Repeatability and Reproducibility of Measures of Bovine Methane Emissions Recorded using a Laser Detector
Authors: Niero Giovanni , Cendron Filippo , Penasa Mauro , De Marchi Massimo , Cozzi Giulio , Cassandro Martino
Published: April 2020

Methane (CH4) emissions represent a worldwide problem due to their direct involvement in atmospheric warming and climate change. Ruminants are among the major players in the global scenario of CH4 emissions, and CH4 emissions are a problem for feed efficiency since enteric CH4 is eructed to the detriment of milk and meat production. The collection of CH4 phenotypes at the population level is still hampered by costly and time-demanding techniques. In the present study, a laser methane detector was used to assess repeatability and reproducibility of CH4 phenotypes, including mean and aggregate of CH4 records, slope of the linear equation modelling the aggregate function, and mean and number of CH4 peak records. Five repeated measurements were performed in a commercial farm on three Simmental heifers, and the same protocol was repeated over a period of three days. Methane emission phenotypes expressed as parts per million per linear meter (ppm × m) were not normally distributed and, thus, they were log-transformed to reach normality. Repeatability and reproducibility were calculated as the relative standard deviation of five measurements within the same day and 15 measurements across three days, respectively. All phenotypes showed higher repeatability and reproducibility for log-transformed data compared with data expressed as ppm × m. The linear equation modelling the aggregate function highlighted a very high coefficient of determination (≥0.99), which suggests that daily CH4 emissions might be derived using this approach. The number of CH4 peaks resulted as particularly diverse across animals and therefore it is a potential candidate to discriminate between high and low emitting animals. Results of this study suggest that laser methane detector is a promising tool to measure bovine CH4 emissions in field conditions.
Welfare Assessment on Pasture: A Review on Animal-Based Measures for Ruminants
Authors: Spigarelli Chiara , Zuliani Anna , Battini Monica , Mattiello Silvana , Bovolenta Stefano
Published: April 2020

Outdoor and extensive farming systems allow animals to behave in a natural way and are often perceived as welfare friendly. Nonetheless, the natural environment poses multiple challenges to the welfare of animals, sometimes hampering their capacity to cope. Welfare assessment in outdoor and extensive systems has been rarely investigated, and little is known about the most appropriate indicators. The aim of this review was to identify animal-based measures of welfare to apply in extensive and pasture-based systems in domestic ruminants. Through the use of a dedicated software for systematic reviews, 810 papers were screened and a total of 52 papers were retained for in-depth analysis. ABM resulting from these papers were initially divided according to the species (cattle and small ruminants, including sheep and goats) and then to four principles: comfort, behavior, feeding and health. The results showed that welfare data were collected applying different methodologies, with an increasing use of sensors in recent years. The need to herd and restrain animals for individual data collection is one of the major constraints to data collection in extensive farming systems. It is suggested that welfare assessment in outdoor/extensive farming systems is carried out by following shared procedures in order to provide evidence of the higher animal welfare claims that these products often imply compared to indoor systems.
Impact of Poultry Farmers’ Participation in Modern Food Retail Markets on Household Dietary Diversity: Lessons from Southeast Nigeria
Authors: Onyeneke Robert Ugochukwu , Emenekwe Chukwuemeka Chinonso , Chidiebere-Mark Nneka Maris , Munonye Jane Onuabuchi , Aligbe Jonathan Ogbeni , Kanu Clementina , Izuogu Chibuzo Uzoma , Njoku Chukwudi Loveday , Uwazie Uwazie Iyke , Uwadoka Christian Obioma , Azuamairo Gillian Chidozie
Published: April 2020

This study analyzed the interrelationships between participation in MFRMs and dietary diversity of poultry farming households in Southeast Nigeria. We used cross-sectional data from poultry farmers in Southeast Nigeria and employed instrumental variable and seemingly unrelated regression models to estimate the impact of MFRM participation and major linkages to poultry farm households’ dietary diversity. The results show that participating in MFRMs, relative to traditional markets, improved poultry farmers’ dietary diversity. Moreover, dietary diversity was positively related to higher poultry farm incomes, higher value of own poultry products consumed, and larger area of vegetable cultivated using poultry droppings as manure. Furthermore, increased poultry farm income, higher value of own poultry products consumed, and larger area of vegetable land cultivated using poultry droppings as manure increased the dietary diversity of the farm households. In contrast, a higher share of poultry production revenue controlled by men reduced household dietary diversity. These findings make clear the potential of improving farming households’ nutrition outcomes by promoting participation in MFRMs and the major impact pathways.
A Distributed Indoor Mapping Method Based on Control-Network-Aided SLAM: Scheme and Analysis
Authors: Tang Jian , Wen Jingren , Qian Chuang
Published: April 2020

Indoor mobile mapping techniques are important for indoor navigation and indoor modeling. As an efficient method, Simultaneous Localization and Mapping (SLAM) based on Light Detection and Ranging (LiDAR) has been applied for fast indoor mobile mapping. It can quickly construct high-precision indoor maps in a certain small region. However, with the expansion of the mapping area, SLAM-based mapping methods face many difficulties, such as loop closure detection, large amounts of calculation, large memory occupation, and limited mapping precision. In this paper, we propose a distributed indoor mapping scheme based on control-network-aided SLAM to solve the problem of mapping for large-scale environments. Its effectiveness is analyzed from the relative accuracy and absolute accuracy of the mapping results. The experimental results show that the relative accuracy can reach 0.08 m, an improvement of 49.8% compared to the mapping result without loop closure. The absolute accuracy can reach 0.13 m, which proves the method’s feasibility for distributed mapping. The accuracies under different numbers of control points are also compared to find the suitable structure of the control network.
Design, Construction, and Modeling of a BAUV with Propulsion System Based on a Parallel Mechanism for the Caudal Fin
Authors: Aparicio-García Cristina Tehaní , Naula Duchi Edisson A. , Garza-Castañón Luis E. , Vargas-Martínez Adriana , Martínez-López J. Israel , Minchala-Ávila Luis I.
Published: April 2020

Traditional propulsion systems for autonomous underwater vehicles (AUVs) have several deficiencies, such as the invasion of the aquatic environment through the generation of noise and damage to the ecosystem, higher energy consumption, and a unidirectional thruster vector. The last characteristic constrains the maneuverability of the vehicle. This paper proposes a 3-DOF spherical 3 universal–cylindrical–universal and 1 spherical joint (3UCU-1S) parallel mechanism coupled to an artificial caudal fin to produce a vectored thruster for a biomimetic AUV (BAUV). First, the design and construction of the prototype are described. Then, the kinematics and dynamics analysis of the parallel mechanism is presented. Finally, a motion study shows the types of movements that can be achieved with the mechanism to perform flapping of the caudal fin in different directions.
Terahertz Single-Pixel Imaging Improved by Using Silicon Wafer with SiO2 Passivation
Authors: She Rongbin , Liu Wenquan , Wei Guanglu , Lu Yuanfu , Li Guangyuan
Published: April 2020

We demonstrate terahertz single-pixel imaging is improved by using a photomodulator based on silicon passivated with SiO 2 . By exploring various SiO 2 thicknesses, we show that the modulation factor of the as-fabricated terahertz photomodulator can reach 0.9, three times that based on bare silicon. This improvement originates from chemical passivation, as well as anti-reflection. Single-pixel imaging experiments based on the compressed sensing method show that reconstructed images adopting the new photomodulator have better quality than the conventional terahertz modulator based on bare silicon. Since the passivation process is routine and low cost, we expect this work will reduce the cost of terahertz photomodulator and single-pixel THz imaging, and advance their applications.
Ultrawideband Low-Profile and Miniaturized Spoof Plasmonic Vivaldi Antenna for Base Station
Authors: Dai Li Hui , Tan Chong , Zhou Yong Jin
Published: April 2020

Stable radiation pattern, high gain, and miniaturization are necessary for the ultra-wideband antennas in the 2G/3G/4G/5G base station applications. Here, an ultrawideband and miniaturized spoof plasmonic antipodal Vivaldi antenna (AVA) is proposed, which is composed of the AVA and the loaded periodic grooves. The designed operating frequency band is from 1.8 GHz to 6 GHz, and the average gain is 7.24 dBi. Furthermore, the measured results show that the radiation patterns of the plasmonic AVA are stable. The measured results are in good agreement with the simulation results.
Dynamic Evaluation of Heat Thefts Due to Different Thermal Performances and Operations between Adjacent Dwellings
Authors: Canale Laura , Battaglia Vittoria , Ficco Giorgio , Puglisi Giovanni , Dell’Isola Marco
Published: April 2020

Apartment position and operation within buildings play a significant role on energy consumption and also on perceived thermal comfort. Dwellings with favorable positions can have significant benefit, also when heated for a limited number of hours, if compared to apartments located in disadvantaged positions (i.e., upper or lower floors or north-oriented). This may be the cause of debates, especially in buildings with central heating, when heat costs are shared among tenants by means of sub-metering systems. In this paper, authors address this issue by studying the “heat thefts” phenomenon in dynamic conditions in a low-insulated building, when the heating system is used unevenly by the tenants (i.e., with different temperatures and/or use). To this end, a social housing building located in Mediterranean climate, where daily temperature excursions and solar heat gains enhance the dynamics of the heat flows, has been chosen as the case-study. The real operation of the building has been simulated in different operational scenarios and the model has been validated against energy consumption data collected experimentally. Results confirm that special allocation and or/compensation strategies should be taken in heat costs allocation in order to avoid accentuating situations of inequalities, especially in low-insulated and/or occasionally heated buildings.
Energy Efficiency and Thermal Performance of Office Buildings Integrated with Passive Strategies in Coastal Regions of Humid and Hot Tropical Climates in Madagascar
Authors: Kameni Nematchoua Modeste , Vanona Jean Christophe , Orosa José A.
Published: April 2020

Researchers have used passive strategies, such as the implementation of thermal insulation and the use of phase change materials (PCM), in several studies, but some problems have not yet been solved. It is the case of showing the real effect of external shading combined with thermal insulation and phase change materials to improve the thermal performance and energy efficiency of office buildings in tropical coastal areas. Another pending problem to be solved is to define the impact produced by passive strategies on the performance of workers in office buildings in coastal zones. It is with a view to answering all these questions that this study was envisaged with the main objective of evaluating, analyzing, comparing, and discussing the effect of thermal insulation and phase change materials on thermal comfort and energy demand in coastal areas of hot and humid tropical climates located in the island of Madagascar. In this sense, hourly climate data for the past 30 years have served as the basis for assessing environmental conditions of future climate. It was found that the PCMs have a more significant effect on the coastal zone of hot climates than humid tropical climates. The results of the statistical analyses showed that the application of passive strategies stabilizes indoor air temperatures to between 23 °C and 28 °C in the offices, which is the recommended comfort range in these regions. In the coastal regions of Madagascar, up to 30% of cooling energy is expected to be reduced by combining the introduction of thermal insulation and PCM materials.
A New Land-Use Dataset for the Weather Research and Forecasting (WRF) Model
Authors: Li Huoqing , Zhang Hailiang , Mamtimin Ali , Fan Shuiyong , Ju Chenxiang
Published: April 2020

The USGS (United States Geological Survey) land-use data used in the Weather Research and Forecasting (WRF) model have become obsolete as they are unable to accurately represent actual underlying surface features. Therefore, this study developed a new multi-satellite remote-sensing land-use dataset based on the latest GLC2015 (Global Land Cover, 2015) land-use data, which had 300 m spatial resolution. The new data were used to update the default USGS land-use dataset. Based on observational data from national meteorological observing stations in Xinjiang, northwest China, a comparison of the old USGS and new GLC2015 land-use datasets in the WRF model was performed for July 2018, where the simulated variables included the sensible heat flux (SHF), latent heat flux (LHF), surface skin temperature (Tsk), two-meter air temperature (T2), wind speed (Winds), specific humidity (Q2) and relative humidity (RH). The results indicated that there were significant differences between the two datasets. For example, our statistical verification results found via in situ observations made by the MET (model evaluation tools) illustrated that the bias of T2 decreased by 2.54%, the root mean square error (RMSE) decreased by 1.48%, the bias of Winds decreased by 10.46%, and the RMSE decreased by 6.77% when using the new dataset, and the new parameter values performed a net positive effect on land–atmosphere interactions. These results suggested that the GLC2015 land-use dataset developed in this study was useful in terms of improving the performance of the WRF model in the summer months.
Is It Time to Rethink Our Weight Loss Paradigms?
Authors: Gentil Paulo , Viana Ricardo Borges , Naves João Pedro , Del Vecchio Fabrício Boscolo , Coswig Victor , Loenneke Jeremy , de Lira Claudio André Barbosa
Published: April 2020

Strategies aiming to promote weight loss usually include anything that results in an increase in energy expenditure (exercise) or a decrease in energy intake (diet). However, the probability of losing weight is low and the probability of sustained weight loss is even lower. Herein, we bring some questions and suggestions about the topic, with a focus on exercise interventions. Based on the current evidence, we should look at how metabolism changes in response to interventions instead of counting calories, so we can choose more efficient models that can account for the complexity of human organisms. In this regard, high-intensity training might be particularly interesting as a strategy to promote fat loss since it seems to promote many physiological changes that might favor long-term weight loss. However, it is important to recognize the controversy of the results regarding interval training (IT), which might be explained by the large variations in its application. For this reason, we have to be more judicious about how exercise is planned and performed and some factors, like supervision, might be important for the results. The intensity of exercise seems to modulate not only how many calories are expended after exercise, but also where they came from. Instead of only estimating the number of calories ingested and expended, it seems that we have to act positively in order to create an adequate environment for promoting healthy and sustainable weight loss.
Regulation of the Single Polar Flagellar Biogenesis
Authors: Kojima Seiji , Terashima Hiroyuki , Homma Michio
Published: April 2020

Some bacterial species, such as the marine bacterium Vibrio alginolyticus, have a single polar flagellum that allows it to swim in liquid environments. Two regulators, FlhF and FlhG, function antagonistically to generate only one flagellum at the cell pole. FlhF, a signal recognition particle (SRP)-type guanosine triphosphate (GTP)ase, works as a positive regulator for flagellar biogenesis and determines the location of flagellar assembly at the pole, whereas FlhG, a MinD-type ATPase, works as a negative regulator that inhibits flagellar formation. FlhF intrinsically localizes at the cell pole, and guanosine triphosphate (GTP) binding to FlhF is critical for its polar localization and flagellation. FlhG also localizes at the cell pole via the polar landmark protein HubP to directly inhibit FlhF function at the cell pole, and this localization depends on ATP binding to FlhG. However, the detailed regulatory mechanisms involved, played by FlhF and FlhG as the major factors, remain largely unknown. This article reviews recent studies that highlight the post-translational regulation mechanism that allows the synthesis of only a single flagellum at the cell pole.
Oxidized LDL Modify the Human Adipocyte Phenotype to an Insulin Resistant, Proinflamatory and Proapoptotic Profile
Authors: Santiago-Fernández Concepción , Martin-Reyes Flores , Tome Mónica , Ocaña-Wilhelmi Luis , Rivas-Becerra Jose , Tatzber Franz , Pursch Edith , Tinahones Francisco J. , García-Fuentes Eduardo , Garrido-Sánchez Lourdes
Published: April 2020

Little information exists in humans on the regulation that oxidized low-density lipoprotein (oxLDL) exerts on adipocyte metabolism, which is associated with obesity and type 2 diabetes. The aim was to analyze the oxLDL effects on adipocytokine secretion and scavenger receptors (SRs) and cell death markers in human visceral adipocytes. Human differentiated adipocytes from visceral adipose tissue from non-obese and morbidly obese subjects were incubated with increasing oxLDL concentrations. mRNA expression of SRs, markers of apoptosis and autophagy, secretion of adipocytokines, and glucose uptake were analyzed. In non-obese and in morbidly obese subjects, oxLDL produced a decrease in insulin-induced glucose uptake, a significant dose-dependent increase in tumor necrosis factor-α (TNF-α), IL-6, and adiponectin secretion, and a decrease in leptin secretion. OxLDL produced a significant increase of Lox-1 and a decrease in Cxcl16 and Cl-p1 expression. The expression of Bnip3 (marker of apoptosis, necrosis and autophagy) was significantly increased and Bcl2 (antiapoptotic marker) was decreased. OxLDL could sensitize adipocytes to a lower insulin-induced glucose uptake, a more proinflammatory phenotype, and could modify the gene expression involved in apoptosis, autophagy, necrosis, and mitophagy. OxLDL can upregulate Lox-1, and this could lead to a possible amplification of proinflammatory and proapoptotic effects of oxLDL.
Electrochemical (Bio)Sensors for Pesticides Detection Using Screen-Printed Electrodes
Authors: Pérez-Fernández Beatriz , Costa-García Agustín , Muñiz Alfredo de la Escosura-
Published: April 2020

Pesticides are among the most important contaminants in food, leading to important global health problems. While conventional techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) have traditionally been utilized for the detection of such food contaminants, they are relatively expensive, time-consuming and labor intensive, limiting their use for point-of-care (POC) applications. Electrochemical (bio)sensors are emerging devices meeting such expectations, since they represent reliable, simple, cheap, portable, selective and easy to use analytical tools that can be used outside the laboratories by non-specialized personnel. Screen-printed electrodes (SPEs) stand out from the variety of transducers used in electrochemical (bio)sensing because of their small size, high integration, low cost and ability to measure in few microliters of sample. In this context, in this review article, we summarize and discuss about the use of SPEs as analytical tools in the development of (bio)sensors for pesticides of interest for food control. Finally, aspects related to the analytical performance of the developed (bio)sensors together with prospects for future improvements are discussed.
Combined Effects of Ethnicity and Education on Burden of Depressive Symptoms over 24 Years in Middle-Aged and Older Adults in the United States
Authors: Assari Shervin
Published: April 2020

Ethnicity and educational attainment are among the major social determinants of depression in the general population. While high education credentials protect individuals against depressive symptoms, this protection may be weaker for ethnic minority groups such as Hispanic Whites compared to the majority group (non-Hispanic Whites). Built on marginalization-related diminished returns (MDRs), the current study used 24-year follow-up data from a nationally representative sample of middle-aged and older adults to explore ethnic variation in the protective effect of education levels against the burden of depressive symptoms over time. Data for this analysis were borrowed from the Health and Retirement Study (HRS 1992–ongoing), a nationally representative longitudinal study. HRS followed 8314 middle-aged and older adults (50+ years old) for up to 24 years. From this number, 763 (9.2%) were Hispanic White, and 7551 (90.8%) were non-Hispanic White Americans. Education level was the independent variable. We had two outcomes. Firstly, using cluster analysis, individuals were categorized to low- and high-risk groups (regarding the burden of depressive symptoms over 24 years); secondly, average depressive symptoms were observed over the 24 years of follow up. Age and gender were the covariates. Ethnicity was the moderator. Linear and logistic regression were used for analysis. Logistic regression showed that, overall, high educational credentials reduced the odds of chronic depressive symptoms over the 24 years of follow-up. Linear regression also showed that higher years of education were associated with lower average depressive symptoms over time. Both models showed statistically significant interactions between ethnicity and graduation, indicating a smaller protective effect of high education against depressive symptoms over the 24 years of follow-up time among Hispanic with respect to non-Hispanic White people. In line with the MDRs, highly educated Hispanic White Americans remain at high risk for depressive symptoms, a risk that is unexpected given their education. The burden of depressive symptoms, however, is lowest for highly educated non-Hispanic White Americans. Policies that exclusively focus on equalizing educational gaps across ethnic groups may fail to eliminate the ethnic gap in the burden of chronic depressive symptoms, given the diminished marginal health return of education for ethnic minorities. Public policies must equalize not only education but also educational quality across ethnic groups. This aim would require addressing structural and environmental barriers that are disproportionately more common in the lives of ethnic minorities across education levels. Future research should test how contextual factors, residential segregation, school segregation, labor market practices, childhood poverty, and education quality in urban schools reduce the health return of educational attainment for highly educated ethnic minorities such as Hispanics.
Key Decision-Makers and Persuaders in the Selection of Energy-Efficient Technologies in EU Residential Buildings
Authors: Camarasa Clara , Heiberger Raphael , Hennes Lena , Jakob Martin , Ostermeyer York , Rosado Leonardo
Published: April 2020

With regard to residential energy use in the European Union (EU), most studies consider potential adopters of the technology (e.g., private owners) as being the sole decision-makers in the technology selection. However, during an integrated decision-making process (e.g., a construction project) multiple stakeholders will interact, influencing each other’s judgement, thereby making it difficult to discern who is affecting the final decision, and to what extent. The goal of this study is to outline the full network of stakeholders involved in the decision-making process, along with their degree of power and interaction in the technology choice. For this purpose, empirical evidence from a multi-country survey is examined using social network analysis (SNA). The information is compared across building typologies, project types and countries (i.e., Italy, Spain, Germany, Poland, the United Kingdom, France, Belgium and the Netherlands). The results demonstrate that, in EU residential buildings, potential adopters of the technology are not the only stakeholders involved in the technology selection. They are in all instances in communication with multiple stakeholders, some of whom also hold a high level of power in the decision (i.e., key persuaders). Furthermore, their level of power and communication varies substantially across building typologies, project types and countries.
L-Glucose: Another Path to Cancer Cells
Authors: Ono Koki , Takigawa Shota , Yamada Katsuya
Published: April 2020

Cancerous tumors comprise cells showing metabolic heterogeneity. Among numerous efforts to understand this property, little attention has been paid to the possibility that cancer cells take up and utilize otherwise unusable substrates as fuel. Here we discuss this issue by focusing on l-glucose, the mirror image isomer of naturally occurring d-glucose; l-glucose is an unmetabolizable sugar except in some bacteria. By combining relatively small fluorophores with l-glucose, we generated fluorescence-emitting l-glucose tracers (fLGs). To our surprise, 2-NBDLG, one of these fLGs, which we thought to be merely a control substrate for the fluorescent d-glucose tracer 2-NBDG, was specifically taken up into tumor cell aggregates (spheroids) that exhibited nuclear heterogeneity, a major cytological feature of malignancy in cancer diagnosis. Changes in mitochondrial activity were also associated with the spheroids taking up fLG. To better understand these phenomena, we review here the Warburg effect as well as key studies regarding glucose uptake. We also discuss tumor heterogeneity involving aberrant uptake of glucose and mitochondrial changes based on the data obtained by fLG. We then consider the use of fLGs as novel markers for visualization and characterization of malignant tumor cells.
Mass Spectrometry Imaging Reveals Neutrophil Defensins as Additional Biomarkers for Anti-PD-(L)1 Immunotherapy Response in NSCLC Patients
Authors: Berghmans Eline , Jacobs Julie , Deben Christophe , Hermans Christophe , Broeckx Glenn , Smits Evelien , Maes Evelyne , Raskin Jo , Pauwels Patrick , Baggerman Geert
Published: April 2020

(1) Background: Therapeutic blocking of the interaction between programmed death-1 (PD-1) with its ligand PD-L1, an immune checkpoint, is a promising approach to restore the antitumor immune response. Improved clinical outcomes have been shown in different human cancers, including non-small cell lung cancer (NSCLC). Unfortunately, still a high number of NSCLC patients are treated with immunotherapy without obtaining any clinical benefit, due to the limitations of PD-L1 protein expression as the currently sole predictive biomarker for clinical use; (2) Methods: In this study, we applied mass spectrometry imaging (MSI) to discover new protein biomarkers, and to assess the possible correlation between candidate biomarkers and a positive immunotherapy response by matrix-assisted laser desorption/ionization (MALDI) MSI in 25 formalin-fixed paraffin-embedded (FFPE) pretreatment tumor biopsies (Biobank@UZA); (3) Results: Using MALDI MSI, we revealed that the addition of neutrophil defensin 1, 2 and 3 as pretreatment biomarkers may more accurately predict the outcome of immunotherapy treatment in NSCLC. These results were verified and confirmed with immunohistochemical analyses. In addition, we provide in-vitro evidence of the immune stimulatory effect of neutrophil defensins towards cancer cells; and (4) Conclusions: With proteomic approaches, we have discovered neutrophil defensins as additional prospective biomarkers for an anti-PD-(L)1 immunotherapy response. Thereby, we also demonstrated that the neutrophil defensins contribute in the activation of the immune response towards cancer cells, which could provide a new lead towards an anticancer therapy.
(002) Oriented Bi2O2CO3 Nanosheets with Enhanced Photocatalytic Performance for Toluene Removal in Air
Authors: Ding Junping , Wang Huanchun , Luo Yidong , Xu Yushuai , Liu Jinsheng , Lin Yuanhua
Published: April 2020

Layer-structured Bi2O2CO3 is a novel photocatalyst for eliminating environmental pollutants. In this work, Bi2O2CO3 nanosheets were synthesized by hydrothermal methods, followed by annealing in nitrogen. (002) oriented Bi2O2CO3 nanosheets were obtained and characterized by XRD, SEM, XPS, BET and UV-Vis diffuse reflectance spectra. Photocatalytic properties were investigated by toluene removal in air, with the assistant of Bi2O2CO3 nanosheets under artificial irradiation. Our results show that Bi2O2CO3 annealed in nitrogen exhibited high full-light-driven photocatalytic activity for toluene photocatalytic decomposition, which may be ascribed to facet orientation evolution during the annealing process and enhanced efficient charge separation. The sample annealed at 150 °C for 8 h (BOC-150-8 h) showed high stability and the highest toluene removal rate, which was up to 99%. The final degradation products were detected by gas chromatography–mass spectrometer (GC-MS) and CO2 was verified to be the primary product. Photocatalytic mineralization of toluene in air over Bi2O2CO3 was proposed. This work may provide a foundation for application of annealed Bi2O2CO3 in indoor air purification.
TMSCl-Catalyzed Tandem Reaction of Dihydroisobenzofuran Acetals with Indoles
Authors: Zhang Ming , Lu Sicong , Li Guofeng , Hong Liang
Published: April 2020

A TMSCl-catalyzed tandem reaction of dihydroisobenzofuran acetals with indoles has been developped, which could provide an efficient and straightforward access to various tetrahydroisoquinolones in moderate to excellent yields. This process involved the first addition of the indoles to acetals, followed by skeletal rearrangement.
Targeting and Crossing the Blood-Brain Barrier with Extracellular Vesicles
Authors: Saint-Pol Julien , Gosselet Fabien , Duban-Deweer Sophie , Pottiez Gwënaël , Karamanos Yannis
Published: April 2020

The blood–brain barrier (BBB) is one of the most complex and selective barriers in the human organism. Its role is to protect the brain and preserve the homeostasis of the central nervous system (CNS). The central elements of this physical and physiological barrier are the endothelial cells that form a monolayer of tightly joined cells covering the brain capillaries. However, as endothelial cells regulate nutrient delivery and waste product elimination, they are very sensitive to signals sent by surrounding cells and their environment. Indeed, the neuro-vascular unit (NVU) that corresponds to the assembly of extracellular matrix, pericytes, astrocytes, oligodendrocytes, microglia and neurons have the ability to influence BBB physiology. Extracellular vesicles (EVs) play a central role in terms of communication between cells. The NVU is no exception, as each cell can produce EVs that could help in the communication between cells in short or long distances. Studies have shown that EVs are able to cross the BBB from the brain to the bloodstream as well as from the blood to the CNS. Furthermore, peripheral EVs can interact with the BBB leading to changes in the barrier’s properties. This review focuses on current knowledge and potential applications regarding EVs associated with the BBB.
Macrophage-Derived Extracellular Vesicle Promotes Hair Growth
Authors: Rajendran Ramya Lakshmi , Gangadaran Prakash , Seo Chang Hoon , Kwack Mi Hee , Oh Ji Min , Lee Ho Won , Gopal Arunnehru , Sung Young Kwan , Jeong Shin Young , Lee Sang-Woo , Lee Jaetae , Ahn Byeong-Cheol
Published: April 2020

Hair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles (MAC-EVs) in promoting hair growth. We studied the proliferation, migration, and expression of growth factors of human-DP cells in the presence or absence of MAC-EVs. Additionally, we tested the effect of MAC-EV treatment on hair growth in a mouse model and human hair follicles. Data from western blot and flow cytometry showed that MAC-EVs were enriched with Wnt3a and Wnt7b, and more than 95% were associated with their membrane. The results suggest that Wnt proteins in MAC-EVs activate the Wnt/β-catenin signaling pathways, which leads to activation of transcription factors (Axin2 and Lef1). The MAC-EVs significantly enhanced the proliferation, migration, and levels of hair-inductive markers of DP cells. Additionally, MAC-EVs phosphorylated AKT and increased the levels of the survival protein Bcl-2. The DP cells treated with MAC-EVs showed increased expression of vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Treatment of Balb/c mice with MAC-EVs promoted hair follicle (HF) growth in vivo and also increased hair shaft size in a short period in human HFs. Our findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss.
Modulation of STAT3 Signaling, Cell Redox Defenses and Cell Cycle Checkpoints by β-Caryophyllene in Cholangiocarcinoma Cells: Possible Mechanisms Accounting for Doxorubicin Chemosensitization and Chemoprevention
Authors: Di Sotto Antonella , Di Giacomo Silvia , Rubini Elisabetta , Macone Alberto , Gulli Marco , Mammola Caterina Loredana , Eufemi Margherita , Mancinelli Romina , Mazzanti Gabriela
Published: April 2020

Cholangiocarcinoma (CCA) is an aggressive group of biliary tract cancers, characterized by late diagnosis, low effective chemotherapies, multidrug resistance, and poor outcomes. In the attempt to identify new therapeutic strategies for CCA, we studied the antiproliferative activity of a combination between doxorubicin and the natural sesquiterpene β-caryophyllene in cholangiocarcinoma Mz-ChA-1 cells and nonmalignant H69 cholangiocytes, under both long-term and metronomic schedules. The modulation of STAT3 signaling, oxidative stress, DNA damage response, cell cycle progression and apoptosis was investigated as possible mechanisms of action. β-caryophyllene was able to synergize the cytotoxicity of low dose doxorubicin in Mz-ChA-1 cells, while producing cytoprotective effects in H69 cholangiocytes, mainly after a long-term exposure of 24 h. The mechanistic analysis highlighted that the sesquiterpene induced a cell cycle arrest in G2/M phase along with the doxorubicin-induced accumulation in S phase, reduced the γH2AX and GSH levels without affecting GSSG. ROS amount was partly lowered by the combination in Mz-ChA-1 cells, while increased in H69 cells. A lowered expression of doxorubicin-induced STAT3 activation was found in the presence of β-caryophyllene in both cancer and normal cholangiocytes. These networking effects resulted in an increased apoptosis rate in Mz-ChA-1 cells, despite a lowering in H69 cholangiocytes. This evidence highlighted a possible role of STAT3 as a final effector of a complex network regulated by β-caryophyllene, which leads to an enhanced doxorubicin-sensitivity of cholangiocarcinoma cells and a lowered chemotherapy toxicity in nonmalignant cholangiocytes, thus strengthening the interest for this natural sesquiterpene as a dual-acting chemosensitizing and chemopreventive agent.