bluuebunny/crossref_metadata_2025 huggingface dataset:
Gene Flow and Recruitment Patterns among Disjunct Populations of Allocasuarina verticillata (Lam.) L.A.S. Johnson
Authors: Zhang Yong , England Nigel , Broadhurst Linda , Li Lan , Zhong Chonglu , Bush David
Published: July 2022

Allocasuarina verticillata (Lam.) L.A.S. Johnson is a widespread species in south-eastern Australia providing vegetation cover, protecting fragile soils and providing food for birds. Understanding the effects of gene flow on the recruitment patterns, genetic differentiation and structure of fragmented populations provides fundamental guidelines to underpin plant conservation strategies and activities. In this study, four spatially disjunct populations of A. verticillata were sampled to explore the effects of population size, reproductive patterns and pollen and seed dispersal on among-population genetic diversity, genetic differentiation and structure, using field survey and microsatellite marker techniques. It was found that stands of A. verticillata were predominantly sexually reproductive, but asexual reproduction through root suckering was an additional mode of reproduction. The reproductive success of A. verticillata is positively correlated with the effective population size rather than actual population size. The reduction in effective population size and increment of spatial isolation resulted in lower genetic diversity and higher inbreeding coefficient of progenies. Moderate pairwise genetic differentiation and weak genetic structure were identified. The results suggest that exogenous, wind-mediated pollen flow provides some maintenance of genetic diversity in the isolated stands. Seed dispersal appears mainly to be over short distances (i.e., within populations), but the infrequent transport of seeds between disjunct locations cannot be ruled out as another factor that may help maintain genetic diversity.
Embedment Strength of Low- and Medium-Density Hardwood Species from Spain
Authors: Cabrera Gonzalo , Moltini Gonzalo , Baño Vanesa
Published: July 2022

The embedment strength is a key parameter in the design of timber connections with metal fasteners. This property can be determined by the equations given by design codes such as the Eurocode 5, which are based on the European Yield Model proposed by Johansen, and it depends on the value of timber density among other parameters. These equations provided by design codes are based on experimental tests performed mainly in softwood species; thus, the objective of this work is to evaluate the embedment strength of two low- and medium-density hardwood species from Spain (poplar—Populus x euroamericana; beech—Fagus sylvatica) in the parallel and perpendicular to grain directions. Four different experimental test configurations were carried out according to EN 383 for each species using two different fasteners: (i) a 9 mm-diameter screw and (ii) a 12 mm-diameter bolt. Results of embedment strength were evaluated according to three different determination methods, and later compared with the current equations provided by Eurocode 5 (EC5) and new ones proposed in the draft of the new Eurocode 5 (prEC5). Results showed that current equations overestimated perpendicular to grain embedment strength for the cases studied, while the equation proposed in prEC5 for screws fitted best perpendicular to grain embedment strength. However, it underestimated the parallel to grain one because it does not consider any difference due to load-to-grain angle (α). Finally, ratios between experimental parallel and perpendicular to grain embedment strength were studied (k90), showing 30% and 44% higher values than the theoretical values resulting from k90 equations of EC5 and prEC5 for beech with screws and bolts, respectively, and 4% and 49% higher than the theoretical values for poplar with screws and bolts, respectively.
Stoichiometric Variation in Soil Carbon, Nitrogen, and Phosphorus Following Cropland Conversion to Forest in Southwest China
Authors: Lu Mengzhen , Liu Kunping , Zhang Lijin , Zeng Fuping , Song Tongqing , Peng Wanxia , Du Hu
Published: July 2022

Soil organic carbon (SOC), nitrogen (N), and phosphorus (P) are three essential soil nutrients for plant growth, and their stoichiometric ratios are already important indices of elemental balance and the soil fertility status in soil ecosystems. The evolution mechanism of the SOC, Total Nitrogen (TN), Total Phosphorus (TP), and stoichiometry following the “conversion of cropland to forest program” (CCFP) in southwest China is not yet clear. Seven different CCFP restoration models, including Zenia insignis (RD), Toona sinensis (XC), Castanea mollissima (BL), Citrus reticulate (GJ), Zenia insignis and Guimu-1 elephant grass (RG), Guimu-1 elephant grass (GM), and abandoned cropland (LH), were chosen to explore changes in the concentration and stoichiometry of the SOC, TN, and TP, and their recovery times, at a depth of 0–100 cm. The results indicate that the SOC and TN concentrations in different restoration models all increased with restoration years in the topsoil, whereas the soil TP concentration remained relatively stable. The soil C:N and C:P ratios increased with increasing restoration years in the topsoil, whereas the N: P ratio was relatively stable over time. After ten years of reforestation, the SOC and TN concentrations decreased as the soil layer increased. The effects of the restoration model on the C: N ratios were greater in shallow soils. Our results suggest a complex reaction of SOC, soil TN, and soil TP concentrations and stoichiometry to the vegetation restoration mode, particularly in the topsoil. This research further improves the understanding of SOC, N, and P interactions and restricted nutrition, and provides relevant theoretical support for vegetation restoration in the southwest karst region.
Growth, Nutrient Accumulation, and Nutritional Efficiency of a Clonal Eucalyptus Hybrid in Competition with Grasses
Authors: Maciel Josiane Costa , Duque Tayna Sousa , Ferreira Evander Alves , Zanuncio José Cola , Plata-Rueda Angélica , Silva Valdevino Pereira , Silva Daniel Valadão , Fernandes Bruno Caio Chaves , Barros Júnior Aurélio Paes , dos Santos José Barbosa
Published: July 2022

Invasive grasses reduce resource availability, mainly nutrients in the soil, and the growth of eucalyptus plants. Efficient management to increase productivity depends on understanding levels of weed interference in eucalyptus plantations. The nutritional efficiency of eucalyptus plants in competition has been evaluated by plant tissue analysis. The objective was to evaluate the growth, relative accumulation of nutrients, and nutritional efficiency of the eucalyptus clonal hybrid I144 (Eucalyptus urophylla × Eucalyptus grandis), in competition with Megathyrsus maximus cv. BRS zuri, Urochloa brizantha cv. marandu, Urochloa decumbens cv. basilisk and in the control (eucalyptus plants without weed competition). The experiment was carried out with a completely randomized design, with four treatments and ten replications. The height, stem diameter, number of leaves, leaf area, dry matter of leaves and stem, nutrient content in leaves and uptake, transport, and N, P, and K utilization efficiency of the eucalyptus clonal hybrid were evaluated at 110 days after transplantation. The growth parameters and relative contents of macro and micronutrients in the eucalyptus clonal hybrid were lower in competition with M. maximus, U. brizantha and U. decumbens. The efficiency of N, P, and K uptake and transport by the eucalyptus clonal hybrid was 29.41 and 7.32% lower in competition with U. decumbens than in the control treatments, respectively. The efficiency of N, P, and K utilization by eucalypts was 13.73, 9.18, and 22.54% lower in competition with M. maximus, U. brizantha, and U. decumbens, respectively. The reduced growth and nutritional parameters of the eucalyptus clonal hybrid were more evident in competition with U. decumbens. Plant tissue analyses efficiently determined the level of competition for nutrients between species. Crop competition with grasses can decrease the efficiency and use of nutrients, which consequently reduces plant development and productivity.
Association of the Female Flight Ability of Asian Spongy Moths (Lymantria dispar asiatica) with Locality, Age and Mating: A Case Study from China
Authors: Akram Muhammad , Hayat Umer , Shi Juan , Anees Shoaib Ahmad
Published: July 2022

The spongy moth (formerly known as a gypsy moth), Lymantria dispar L. (Lepidoptera: Erebidae), is a polyphagous pest that defoliates various species of trees in the genera Populus, Salix, Quercus, Acer and Pinus. Egg masses of Asian spongy moths (Lymantria dispar asiatica) were obtained from five geographically distinct locations in China (Chengdu, Xifeng, Heilongjiang, Kuduer and Longhua regions) and were carried to the lab for incubation and rearing larvae up to adult emergence. The aim was to see how locality, age and mating status influenced the flying activity of female Asian spongy moths. The findings demonstrate that age significantly affects L. dispar asiatica flying activity, with flight period and distance decreasing dramatically as age increases. Locality also significantly impacts flight ability, and mating status does not affect the L. dispar asiatica female flight ability (p > 0.05). The maximum speed was observed for females from Xifeng (7.19 ± 0.34 km/h). The females from Heilongjiang were recorded as having the maximum average speed (2.35 ± 0.08 km/h). Females from Xifeng exhibited the maximum flight duration and distance (0.94 ± 0.04 h and 1.87 ± 0.18 km). Morphological features, i.e., thorax width (cm) and wing loading, significantly influenced the L. dispar asiatica flight ability (R2 = 0.93 and R2 = −0.98). The overall pattern observed for female L. dispar asiatica flight duration was Xifeng > Kuder > Chengdu > Heilongjiang > Longhua, and for flight distance, it was Xifeng > Longhua > Heilongjiang > Kuder > Chengdu. This research offers detailed information on the dispersion rate of Spongy moths in a known or newly introduced habitat, which can help us limit the spread of this polyphagous pest.
A National Map of Snag Hazard to Reduce Risk to Wildland Fire Responders
Authors: Riley Karin L. , O’Connor Christopher D. , Dunn Christopher J. , Haas Jessica R. , Stratton Richard D. , Gannon Benjamin
Published: July 2022

Falling trees and tree fragments are one of the top five causes of fatalities for wildland fire responders. In six out of ten recent years, at least one fatality from a tree strike has occurred while a fire responder was on duty, and others were injured. We used TreeMap, a national map of forest characteristics, including individual tree height, diameter, and status (live or dead), to generate a map of snag hazard for forested areas of the continental U.S. at 30 × 30 m resolution. Snag hazard was classified into categories of low, moderate, high, or extreme based on snag density and height. Within-class accuracy was as high at 86%, suggesting that the Snag Hazard map can help wildland fire managers identify and avoid exposing fire responders to hazardous conditions. Accuracy was higher outside recently disturbed areas (88%) than inside (79%), perhaps reflecting strong spatial patterns and heterogeneity of mortality within disturbed areas. The Snag Hazard map is a frequently requested product from the Forest Service’sRisk Management Assistance Group. The goal of RMA is to provide analytics to decision makers and fire leadership to facilitate risk-informed decision-making to improve safety, effectiveness, and outcomes. We present a case study showing how the Snag Hazard 2016 map was used to inform fire responders during an active wildfire incident in California during the 2020 fire season.
An Evaluation of National Park System Pilot Area Using the AHP-Delphi Approach: A Case Study of the Qianjiangyuan National Park System Pilot Area, China
Authors: Zhuang Qianda , Wang Lin , Zheng Guoquan
Published: July 2022

With the national park as an important measure of ecological protection, China has started 10 national park system pilot areas. However, the research on the comprehensive evaluation of national park construction and development is insufficient. The main purpose of this study was to establish a comprehensive evaluation index system for the pilot area of the national park system. The method of the Analytic Hierarchy Process combined with the Delphi method by ten relevant experts was used. The comprehensive evaluation index system for the national park system pilot areas was composed of the objective level and the criteria level which includes three items: natural resource conditions; research, education and recreation functions; and protection and management conditions. The indicator level involved seven items and the factor level included 31 items. Furthermore, the weight of each item in these levels was obtained through the Delphi method by the judgement of selected experts. The Qianjiangyuan National Park System Pilot Area (QNPSPA) was selected as the study case for empirical research. The score of the comprehensive evaluation of the QNPSPA was 90.801, which belongs to Grade I in the five catalogues, indicating its comprehensive construction level was very high and its protection and management measures were efficient. However, significant deficiencies also existed in the area suitability, recreation facilities and production, natural resource property rights, human landscape value and ecosystem integrity. To address these problems, five suggestions were proposed. For example, strengthening cross-administrative cooperation and communication, improving the construction of recreational facilities, etc. The evaluation framework proposed in this study could play a positive role in the construction evaluation of the pilot areas of national parks in China and is conducive to promoting the evaluation research of national parks in China and promoting the development of conservation and construction.
Measuring the Tree Height of Picea crassifolia in Alpine Mountain Forests in Northwest China Based on UAV-LiDAR
Authors: Chen Siwen , Nian Yanyun , He Zeyu , Che Minglu
Published: July 2022

Forests in alpine mountainous regions are sensitive to global climate change. Accurate measurement of tree height is essential for forest aboveground biomass estimation. Unmanned aerial vehicle light detection and ranging (UAV-LiDAR) in tree height estimation has been extensively used in forestry inventories. This study investigated the influence of varying flight heights and point cloud densities on the extraction of tree height, and four flight heights (i.e., 85, 115, 145, and 175 m) were set in three Picea crassifolia plots in the Qilian Mountains. After point cloud data were classified, tree height was extracted from a canopy height model (CHM) on the basis of the individual tree segmentation. Through comparison with ground measurements, the tree height estimations of different flight heights and point cloud densities were analyzed. The results indicated that (1) with a flight height of 85 m, the tree height estimation achieved the highest accuracy (R2 = 0.75, RMSE = 2.65), and the lowest accuracy occurred at a height of 175 m (R2 = 0.65, RMSE = 3.00). (2) The accuracy of the tree height estimation decreased as the point cloud density decreased. The accuracies of tree height estimation from low-point cloud density (R2 = 0.70, RMSE = 2.75) and medium density (R2 = 0.69, RMSE = 2.80) were comparable. (3) Tree height was slightly underestimated in most cases when CHM-based segmentation methods were used. Consequently, a flight height of 145 m was more applicable for maintaining tree height estimation accuracy and assuring the safety of UAVs flying in alpine mountain regions. A point cloud density of 125–185 pts/m2 can guarantee tree height estimation accuracy. The results of this study could potentially improve tree height estimation and provide available UAV-LiDAR flight parameters in alpine mountainous regions in Northwest China.
Northern Provenances of Silver Fir Differ with Acclimation to Contrasting Light Regimes
Authors: Robakowski Piotr , Łukowski Adrian , Ye Zi-Piao , Kryszewski Adam , Kowalkowski Wojciech
Published: July 2022

Phenotypic differentiation of Abies alba was identified among selected provenances originating from the northern edge of this species’ natural geographic range. We posited that although the seed sources of the study populations were not geographically distant, progenies would differ with respect to growth, needle pigment concentration, and values of photochemical parameters in response to contrasting light conditions. Potted seedlings of six provenances were grown for two seasons under low light (LL, 40% of full irradiance) or high light (HL, 100% of full irradiance). The results showed that the provenances differed in diameter at root collar (DRC) and in relative growth rates of height (RGR). Seedlings grown in full light had a greater mean value of DRC than those in LL. LL increased total needle chlorophyll (Chltot) and carotenoid concentrations compared with HL, and Chltot was modified by the provenance of the seedlings. The provenance “Syców”, localized at the northern limit of the species range, showed the lowest value of maximal electron transfer rate (ETRmax) and lower values of growth parameters compared with the other study provenances. Relative growth rate, Chltot, and non-photochemical quenching of fluorescence (NPQ) were correlated with some climatic characteristics of seed stands and formed clusters indicating the local adaptation of the study populations. These results provide evidence of low intraspecific A. alba differentiation in terms of growth dynamics, photochemical capacity, and tolerance to different light regimes. The intraspecific diversity found in our study among the northern lead populations could be, however, important for the natural expansion of this species further north and for the selection of provenances to be used in the programme of the A. alba restoration in the Sudety Mountains, southern Poland, where decline of this species has been observed.
Production and Potential Genetic Pathways of Three Different Siderophore Types in Streptomyces tricolor Strain HM10
Authors: Rehan Medhat , Barakat Hassan , Almami Ibtesam S. , Qureshi Kamal A. , Alsohim Abdullah S.
Published: July 2022

Siderophores are iron-chelating low-molecular-weight compounds that bind iron (Fe3+) with a high affinity for transport into the cell. The newly isolated strain Streptomyces tricolor HM10 secretes a pattern of secondary metabolites. Siderophore molecules are the representatives of such secondary metabolites. S. tricolor HM10 produces catechol, hydroxamate, and carboxylate types of siderophores. Under 20 μM FeCl3 conditions, S. tricolor HM10 produced up to 6.00 µg/mL of catechol siderophore equivalent of 2,3-DHBA (2,3-dihydroxybenzoic acid) after 4 days from incubation. In silico analysis of the S. tricolor HM10 genome revealed three proposed pathways for siderophore biosynthesis. The first pathway, consisting of five genes, predicted the production of catechol-type siderophore similar to petrobactin from Bacillus anthracis str. Ames. The second proposed pathway, consisting of eight genes, is expected to produce a hydroxamate-type siderophore similar to desferrioxamine B/E from Streptomyces sp. ID38640, S. griseus NBRC 13350, and/or S. coelicolor A3(2). The third pathway exhibited a pattern identical to the carboxylate xanthoferrin siderophore from Xanthomonas oryzae. Thus, Streptomyces strain HM10 could produce three different types of siderophore, which could be an incentive to use it as a new source for siderophore production in plant growth-promoting, environmental bioremediation, and drug delivery strategy.
Wildfires in the Siberian Arctic
Authors: Kharuk Viacheslav I. , Dvinskaya Maria L. , Im Sergei T. , Golyukov Alexei S. , Smith Kevin T.
Published: July 2022

Wildfires are increasingly understood as an ecological driver within the entire Arctic biome. Arctic soils naturally store large quantities of C, as peat has formed throughout the Holocene. For the Siberian Arctic, we used observations from the MODIS remote sensing instrument to document changes in frequency, geographic extent, and seasonal timing of wildfires as well as vegetation productivity (GPP, NPP, EVI). We also used correlation and regression analysis to identify environmental factors of temperature, precipitation, and lightning occurrence associated with these changes. For the Siberian Arctic as a whole, we found that the decadal frequency of wildfire tripled from the 2001–2010 to the 2011–2020 periods. Increased decadal frequency was accompanied by the increased extent of the burnt area by a factor of 2.6. This increase in fire frequency and extent was not uniform, with the greatest increase in western Siberia with no marked increase for the Siberian Far East. These changes were accompanied by the northward migration of the northern limit of wildfire occurrence and an increase in duration of the wildfire season. We found that annual fire frequency and the extent of burnt areas were related to various combinations of seasonal air temperature, precipitation, ground moisture, and lightning frequency. After fires, vegetation productivity rapidly recovered to pre-fire levels. The northward spread of wildfire into the tundra will release carbon long-stored as peat. The enhanced vegetation productivity, rapid recovery of carbon fixation for burnt areas and the northward migration of boreal forest tree species may offset that release and maintain the current status of the Siberian Arctic as a C sink. Increased wildfire and loss of permafrost may threaten ongoing settlement and industrialization, particularly for western Siberia.
Norwegian Consumers’ Skepticism towards Smoke-Flavoring of Salmon—Is It for Real?
Authors: Waldenstrøm Lene , Wahlgren Marte Berg , Strand Åse , Lerfall Jørgen , Gaarder Mari Øvrum
Published: July 2022

The aim of the present study was to explore Norwegian consumers’ attitudes toward smoke-flavoring of cold smoked salmon (CSS), by conducting a digital survey and focus group discussions. Some of the smoke-flavoring techniques, like atomized purified condensed smoke, is considered healthier than conventional smoking. Manufacturers of CSS are, however, hesitant to use all kinds of smoke-flavoring due to expected consumer skepticism. In the digital survey, the expected skepticism was confirmed (n = 487). Only 15% of the respondents were positively oriented toward smoke-flavoring of CSS. The selection criterion for the focus group discussion was based on the results from the survey and resulted in three attitude-oriented focus groups (negative, neutral, and positive). The negative focus group considered smoke-flavored CSS to be unnatural and out of the question to buy or eat. Unlike the negative focus group, the neutral group was curious to learn more and open to potential smoke-flavor benefits. However, credible information or science was crucial to influence CSS choice. Future studies may investigate whether the existent of this large group of curious neutral consumers (47% of the respondents) influence manufacturers (of CSS) skepticism or how various types of product information could steer consumer acceptance of smoke-flavoring of CSS.
Emerging Trends for Nonthermal Decontamination of Raw and Processed Meat: Ozonation, High-Hydrostatic Pressure and Cold Plasma
Authors: Roobab Ume , Chacha James S. , Abida Afeera , Rashid Sidra , Muhammad Madni Ghulam , Lorenzo Jose Manuel , Zeng Xin-An , Aadil Rana Muhammad
Published: July 2022

Meat may contain natural, spoilage, and pathogenic microorganisms based on the origin and characteristics of its dietary matrix. Several decontamination substances are used during or after meat processing, which include chlorine, organic acids, inorganic phosphates, benzoates, propionates, bacteriocins, or oxidizers. Unfortunately, traditional decontamination methods are often problematic because of their adverse impact on the quality of the raw carcass or processed meat. The extended shelf-life of foods is a response to the pandemic trend, whereby consumers are more likely to choose durable products that can be stored for a longer period between visits to food stores. This includes changing purchasing habits from “just in time” products “for now” to “just in case” products, a trend that will not fade away with the end of the pandemic. To address these concerns, novel carcass-decontamination technologies, such as ozone, high-pressure processing and cold atmospheric plasma, together with active and clean label ingredients, have been investigated for their potential applications in the meat industry. Processing parameters, such as exposure time and processing intensity have been evaluated for each type of matrix to achieve the maximum reduction of spoilage microorganism counts without affecting the physicochemical, organoleptic, and functional characteristics of the meat products. Furthermore, combined impact (hurdle concept) was evaluated to enhance the understanding of decontamination efficiency without undesirable changes in the meat products. Most of these technologies are beneficial as they are cost-effective, chemical-free, eco-friendly, easy to use, and can treat foods in sealed packages, preventing the product from post-process contamination. Interestingly, their synergistic combination with other hurdle approaches can help to substitute the use of chemical food preservatives, which is an aspect that is currently quite desirable in the majority of consumers. Nonetheless, some of these techniques are difficult to store, requiring a large capital investment for their installation, while a lack of certification for industrial utilization is also problematic. In addition, most of them suffer from a lack of sufficient data regarding their mode of action for inactivating microorganisms and extending shelf-life stability, necessitating a need for further research in this area.
Safety Assessment of One Lactiplantibacillus plantarum Isolated from the Traditional Chinese Fermented Vegetables—Jiangshui
Authors: Ou Dexin , Ling Na , Wang Xihao , Zou Yanyan , Dong Jingjing , Zhang Danfeng , Shen Yizhong , Ye Yingwang
Published: July 2022

Lactiplantibacillus plantarum is a kind of extensively utilized probiotic species, which plays a critical role in the prevention of pathogenic bacteria and development of functional probiotics. Our group previously isolated one Lactiplantibacillus from Jiang Shui, a traditional Chinese fermented vegetable, which remarkably inhibited the growth of Aspergillus flavus. Herein, the safety of this isolate was assessed to ensure its application feasibility in food industry. Firstly, the phenotypic analyses including tolerance to low pH and bile salt, aggregation ability, and hemolytic activity detection, indicated the isolate could survive and colonize in the gastrointestinal tract, without hemolysin activity. The susceptibilities of the isolate to eight antibiotics and the absence of most resistance genes were demonstrated by agar disk diffusion and PCR, respectively. Furthermore, no mortality or toxicity was observed in mice by in vivo tests using gross autopsy, hematology, serum biochemistry, and HE-staining. Taken together, this study demonstrated the safety of Lactiplantibacillus plantarum WYH as a probiotic strain in terms of phenotypic analyses, absence of antimicrobial resistance and toxin-related genes, as well as mice toxicity test, while supported the prospect of applying isolate in suppression of fungal growth and mycotoxin biosynthesis.
Numerical Analysis on Heat Characteristics of the Ventilation Basket for Fresh Tea Leaves
Authors: Zeng Zhixiong , Jiang Yihong , Ma Chengying , Chen Jin , Zhang Xiaodan , Lin Jicheng , Liu Yanhua , Guo Jiaming
Published: July 2022

Plastic baskets are commonly used as containers for fresh tea leaves during storage and transport after harvest. Nevertheless, there are significant challenges in controlling the core temperature of the basket since fresh tea leaves still maintain a certain degree of respiration after being harvested, with extremely high temperatures being the major factor for the color change of fresh tea leaves. A numerical model was developed to improve the temperature control of the plastic basket, by which the influence of different structural parameters on the core temperature in the plastic baskets with fresh tea leaves was analyzed. The accuracy of the model in predicting airflow and temperature distributions was validated against experimental data. The maximum RMSE was 1.158 °C and the maximum MRE was 5.410% between the simulated and test temperature value. The maximum deviation between the simulated velocity and test velocity was 0.11 m/s, the maximum RE was 29.05% and the maximum SD was 0.024. The results show that a plastic basket with a ventilation duct efficiently decreased the temperature of the fresh tea leaves and significantly affected the heat transfer between the fresh tea leaves and the ambient air compared to the plastic basket without a ventilation duct. Furthermore, the effect on the heat transfer was further expanded by the use of a plastic basket with a ventilation duct when the plastic baskets were stacked. The maximum temperature differences were 0.52 and 0.40 according to the stacked and single-layer products, respectively. The ambient temperature and the bulk density of the fresh tea leaves have a significant influence on the core temperature.
Metabolic Food Waste as Food Insecurity Factor—Causes and Preventions
Authors: Balan Ioana Mihaela , Gherman Emanuela Diana , Brad Ioan , Gherman Remus , Horablaga Adina , Trasca Teodor Ioan
Published: July 2022

The Metabolic Food Waste MFW (kg of food) first developed in 2016 as a new indicator by Serafini and Toti, indicates the amount of food consumed above the nutritional requirements, and the impact of this overconsumption on the environment. It is necessary to identify the causes and to develop potential methods to prevent and reduce MFW, at the same time as increasing consumer awareness about unsustainable diets and changing diet habits towards more environmentally conscious consumption patterns. This study was conducted by collecting and analysing existing reports and studies regarding nutritional requirements, consumer behaviour related to food consumption and food waste, environmental impacts of food waste, and the concept of Metabolic Food Waste. The process of data collection involved searching the studies available online, using keywords related to the subject of MFW and overconsumption. The references in the initial studies consulted were also analysed in order to further identify new data relevant to overconsumption and MFW. The materials studied and analysed related to the environmental impact of MFW were published by E. Toti and M. Serafini in 2016 and 2019; additionally, in order to understand the causes of overconsumption numerous studies were reviewed regarding consumer behaviour, the relationship between economic development and overconsumption, mental health and dietary habits, physical context and dietary habits, genetic predisposition, also childhood and early adulthood environment. By analysing and corroborating external data available for food waste, nutritional requirements, and the environmental impact of food waste and consumer behaviour, we identified as primary causes for MFW the lack of nutritional education and little understanding of the nutritional requirements amongst all categories of consumers, poor access to appropriate food resources or reduced availability of fresh produced food. We conclude that for the quantification of the negative impact of MFW on both the environment and human health we need decisive action to raise consumer awareness for healthy and sustainable diets, together with a uniform worldwide distribution system for nutritious food.
The Effect of Berry Pomace on Quality Changes of Beef Patties during Refrigerated Storage
Authors: Tarasevičienė Živilė , Čechovičienė Indrė , Paulauskienė Aurelija , Gumbytė Milda , Blinstrubienė Aušra , Burbulis Natalija
Published: July 2022

This study aims to evaluate the ability of raspberry and blackberry pomace to inhibit lipid oxidation and prolong the refrigerated storage of beef patties. Berry pomace was incorporated into beef patties at the concentration of 1, 3, and 5%. Packed patties were stored for 9 days at 4 °C temperature and the quality of the meat was evaluated on the 0, 3rd, 6th, and 9th day. The natural mass loss during storage, the pH as well as the lipid oxidation were evaluated by thiobarbituric acid-reactive substance (TBARS) method. GC was used to determine the amount of fatty acids and e-nose, based on ultrafast gas chromatography, was used for the determination of volatile organic compounds in beef patties before and after the storage. The highest mass loss during refrigerated storage was observed in the control beef patties, while the berry pomace absorbed water and reduced the loss. The pomace additive influenced the decrease in the patties pH during the storage. Berry pomace can be very effective in relation to lipid oxidation, and as little as 1% of berry pomace influenced the decrease in the TBAR’s values in the patties stored for nine days by 3.06 and 2.42 times, depending on the pomace compared to the control patties. The use of berry pomace in meat products can reduce lipid oxidation, increase their fiber content and act as a thickener, as well as contribute to the usage of agri-food by-products.
Hypoallergenic Wheat Line (1BS-18H) Lacking ω5-Gliadin Induces Oral Tolerance to Wheat Gluten Proteins in a Rat Model of Wheat Allergy
Authors: Yamada Yukinori , Yokooji Tomoharu , Kunimoto Kyohei , Inoguchi Koki , Ogino Ryohei , Taogoshi Takanori , Morita Eishin , Matsuo Hiroaki
Published: July 2022

The early ingestion of food can prevent the onset of food allergy related to inducing oral tolerance (OT). We developed the Hokushin wheat line as a hypoallergenic wheat (1BS-18H) lacking ω5-gliadin, a major allergen of wheat-dependent exercise-induced anaphylaxis (WDEIA). The 1BS-18H wheat had lower ability of sensitization for ω5-gliadin compared with Hokushin wheat. Here, we evaluated the induction of OT to gluten and ω5-gliadin by the early consecutive ingestion of 1BS-18H gluten using a rat model of wheat allergy. Rats were subcutaneously immunized with commercial gluten or native ω5-gliadin following the daily oral administration of gluten. The daily oral administration of 1BS-18H gluten for 5 days before immunization suppressed the increase in gluten- or ω5-gliadin-specific IgE and IgG1 antibodies induced by immunization to a level similar to Hokushin gluten. Intravenous challenge with gluten or ω5-gliadin did not decrease the rectal temperature in rats with OT induced by 1BS-18H or Hokushin gluten, although it was decreased in non-OT rats. In conclusion, the early consecutive ingestion of 1BS-18H wheat before sensitization induced OT to gluten and ω5-gliadin. These findings support the benefit of 1BS-18H wheat to prevent wheat allergy including WDEIA by consecutive ingestion in humans.
Olive Pomace Oil versus High Oleic Sunflower Oil and Sunflower Oil: A Comparative Study in Healthy and Cardiovascular Risk Humans
Authors: González-Rámila Susana , Mateos Raquel , García-Cordero Joaquín , Seguido Miguel A. , Bravo-Clemente Laura , Sarriá Beatriz
Published: July 2022

Olive pomace oil (OPO) is mainly a source of monounsaturated fat together with a wide variety of bioactive compounds, such as triterpenic acids and dialcohols, squalene, tocopherols, sterols and aliphatic fatty alcohols. To date, two long-term intervention studies have evaluated OPO’s health effects in comparison with high oleic sunflower oil (HOSO, study-1) and sunflower oil (SO, study-2) in healthy and cardiovascular risk subjects. The present study integrates the health effects observed with the three oils. Two randomized, blinded, cross-over controlled clinical trials were carried out in 65 normocholesterolemic and 67 moderately hypercholesterolemic subjects. Each study lasted fourteen weeks, with two four-week intervention phases (OPO versus HOSO or SO), each preceded by a three-week run-in or washout period. Regular OPO consumption reduced total cholesterol (p = 0.017) and LDL cholesterol (p = 0.018) levels as well as waist circumference (p = 0.026), and only within the healthy group did malondialdehyde (p = 0.004) levels decrease after OPO intake versus HOSO. Contrarily, after the SO intervention, apolipoprotein (Apo) B (p < 0.001) and Apo B/Apo A ratio (p < 0.001) increased, and to a lower extent Apo B increased with OPO. There were no differences between the study groups. OPO intake may improve cardiometabolic risk, particularly through reducing cholesterol-related parameters and waist circumference in healthy and hypercholesterolemic subjects.
Local Fractional Homotopy Perturbation Method for Solving Coupled Sine-Gordon Equations in Fractal Domain
Authors: Chen Liguo , Liu Quansheng
Published: July 2022

In this paper, the coupled local fractional sine-Gordon equations are studied in the range of local fractional derivative theory. The study of exact solutions of nonlinear coupled systems is of great significance for understanding complex physical phenomena in reality. The main method used in this paper is the local fractional homotopy perturbation method, which is used to analyze the exact traveling wave solutions of generalized nonlinear systems defined on the Cantor set in the fractal domain. The fractal wave with fractal dimension ε=ln2/ln3 is numerically simulated. Through numerical simulation, we find that the obtained solutions are of great significance to explain some practical physical problems.
Genome-Wide Association Study Identified a Quantitative Trait Locus and Two Candidate Genes on Sus scrofa Chromosome 2 Affecting Vulvar Traits of Suhuai Pigs
Authors: Yin Yanzhen , Hou Liming , Liu Chenxi , Li Kaijun , Guo Hao , Niu Peipei , Li Qiang , Huang Ruihua , Li Pinghua
Published: July 2022

Vulvar size and angle are meaningful traits in pig production. Sows with abnormal vulva generally show reproductive disorders. In order to excavate candidate loci and genes associated with pig’s vulvar traits, 270 Suhuai pigs with vulvar phenotype were genotyped by a porcine single nucleotide polymorphisms (SNP) Chip. Then, Chip data were imputed using resequenced data of 30 Suhuai pigs as a reference panel. Next, we estimated the heritability and performed a genome-wide association study (GWAS) for vulvar traits. The heritabilities for the traits vulvar length (VL), vulvar width (VW) and vulvar angle (VA) in this pig population were 0.23, 0.32 and 0.22, respectively. GWAS based on Chip data identified nine significant SNPs on the Sus scrofa chromosomes (SSC) 2, 7, 9 and 13 for VL or VW. GWAS based on imputed data identified 11 new quantitative trait loci (QTL) on SSC1, 2, 7, 8, 9, 11, 13, 16 and 17 for VL or VW. The most significant QTL for VL on SSC2 were refined to a 3.48–3.97 Mb region using linkage disequilibrium and linkage analysis (LDLA). In this refined region, FGF19 and CCND1, involved in the development of the reproductive tract, cell growth and vulvar cancer, could be new candidate genes affecting VL. Our results provided potential genetic markers for the breeding of vulvar traits in pigs and deepened the understanding of the genetic mechanism of vulvar traits.
Association of TCF7L2, CASC8 and GREM1 Polymorphisms in Patients with Colorectal Cancer and Type II Diabetes Mellitus
Authors: Mitroi Anca Florentina , Leopa Nicoleta , Dumitru Eugen , Brînzan Costel , Tocia Cristina , Dumitru Andrei , Popescu Răzvan Cătălin
Published: July 2022

Background: The aim of the study is to explore the association between the TCF7L2 rs7903146, CASC8 rs6983267 and GREM1 rs16969681 polymorphisms in patients diagnosed with type 2 diabetes mellitus (T2DM) and colorectal cancer. Methods: Sixty individuals were enrolled in this case-control study: thirty with colorectal cancer and type II diabetes mellitus (T2DM) and thirty healthy control individuals. Real-time PCR was used to determine the genotypes of TCF7L2 rs7903146, CASC8 rs 6983267 and GREM1 rs16969681 in patients with CRC and T2DM and in patients without T2DM and CRC. The Hardy–Weinberg equilibrium was determined in the control group for the genotype distribution of every polymorphism. Results: People carrying the TT genotype of rs7903146, rs6983267 and rs1696981 had a significant association with T2DM and CRC. Moreover, the people with the TT genotype of rs1696981 had a greater risk for T2DM and CRC (OR = 7, CI 0.397–23.347). Conclusions: TCF7L2 rs7903146, CASC8 rs6983267 and GREM1 rs16969681 could be risk factors for the association of T2DM with CRC.
Identification and Validation of an m7G-Related lncRNAs Signature for Prognostic Prediction and Immune Function Analysis in Endometrial Cancer
Authors: Sun Jiani , Li Li , Chen Hong , Gan Lei , Guo Xiaoqing , Sun Jing
Published: July 2022

Background: N7-methylguanosine is a novel kind of internal modification that is widespread in human mRNA. The relationship between m7G-related lncRNAs (MRL) and endometrial cancer remains unknown. The aim of our study is to explore a predictive prognosis MRL signature in endometrial cancer and identify the underlying biological mechanism. Methods: We obtained RNA-seq profiles, clinical data, and information on somatic mutations from the TCGA database and obtained m7G-related genes from a previous study. MRLs were identified through a co-expression network. The prognostic model was constructed based on 10 m7G-related lncRNAs. Differentially expressed genes between low- and high-risk groups were identified for further analysis, consisting of functional enrichment analysis, immune function analysis, somatic mutation analysis, and potential drugs exploration. Results: We constructed a 10-MRLs signature. According to the risk score, the signature was classified into high- and low-risk groups. The signature had a reliable capacity for predicting the prognosis of endometrial cancer patients. The findings about differentially expressed genes were also of great significance for therapeutic treatments for endometrial cancer and gave novel insights into exploring the underlying molecular mechanism. Conclusion: The prognostic model based on 10 MRLs is a reliable and promising approach for predicting clinical outcomes and suggesting therapeutic methods for endometrial cancer patients.
Variety and Distribution of Diatom-Based Sea Ice Proxies in Antarctic Marine Sediments of the Past 2000 Years
Authors: Allen Claire S. , Weich Zelna C.
Published: July 2022

Antarctic sea ice is an essential component of the global climate system. Reconstructions of Antarctic sea ice from marine sediment cores are a vital resource to improve the representation of Antarctic sea ice in climate models and to better understand natural variability in sea ice over centennial and sub-centennial timescales. The Thomas et al. (2019) review of Antarctic sea ice reconstructions from ice and marine cores highlighted the prominence of diatom-based proxies in this research. Here, focusing solely on the diatom-based proxy records in marine sediments, we review the composition of proxies, their advantages and limitations, as well as the spatial and temporal cover of the records over the past 2 ka in order to assess the scope for future assimilation and standardization. The archive comprises 112 records from 68 marine cores, with proxies based on more than 30 different combinations of diatom taxa as well as the relatively new, highly branched isoprenoid (HBI) biomarkers.
The “In-Between Land” of Suspicion and Ambiguity: Plotting the MS Estonia Shipwreck
Authors: Sorokin Siim
Published: July 2022

The present article is multidisciplinary, drawing on and synthesizing narrative media theories, philosophy of epistemology, conspiracy theory research, and creativity studies. I will explore the following central theoretical problem: whether it is conceptually enriching to (i) further develop the notion of and hence advance the scholarship in “conspiracy theorizing” and (ii) in doing so, would it be productive to ponder the role of peoples’ affective state of suspicion in engaging with ambiguous representations, something that is thrown into especially sharp relief by the conspiracist discourse. Accordingly, my point of departure is the concept of ambiguity and the related semantic field (including its antithesis, closure). Hereby, the concept of suspicion is introduced and treated as a creativity-enhancing, productive affect rooted in narrative thinking and construction. In particular, a specific manifestation of ambiguity apparent in digital sense-making discourses is foregrounded—a self-reproduced ambiguity. These dynamics are explored in the context of, while aspiring to overcome the scholarly emphasis on its negative valence, the practice of “conspiracy theorizing”. This popular practice is hence reconceptualized as contra-plotting. It is understood as a form of sense-making undertaken by the plotters of suspicion in challenging official explanations found unsatisfying and straining one’s belief. Such activity emerges and becomes instrumental in the face of explanatory uncertainty, such as the unsolved nature (“the how”) of the shipwreck, and is posited to be an individual and collaborative creative construction characterized by “continual interpretation”. For, as I will argue, the functional outcome of contra-plotting is to self-reproduce—not to obtain closure for the—ambiguity. Motivated by the suspicious stance, it is a necessary operative mode of such interpretation itself. In attempting to overcome their suspicions about official explanations, plotters inadvertently also ‘plot’ suspicion. Consequently, such an interpretative process corresponding to disambiguation plotting always feeds back into its own ever-expanding (narrative) ‘middle’, searching for yet immediately disregarding, as if by design, any final crystallized ‘truth’. In this context, the perhaps more understated meaning of “to interpret”—namely, to creatively supplement “deficiencies” (supplentio)—may gain in conceptual relevance. In staking the proposed theoretical apparatus, I will draw on my preliminary findings from analytical work on ‘real-time’ digital discussions—observable as a chronological forum archive—on the 1994 shipwreck of the cruise ferry MS Estonia. In order to instrumentalize the outlined tentative theoretical vocabulary, an interpretative close reading of posts from different time periods from the conspiracist forum Para-Web will be provided. This analysis combines textual and narrative analyses. The article ends with some concluding thoughts and aims for further research.