bluuebunny/crossref_metadata_2025 huggingface dataset:
On Parameter Identification for Reaction-Dominated Pore-Scale Reactive Transport Using Modified Bee Colony Algorithm
Authors: Grigoriev Vasiliy V. , Iliev Oleg , Vabishchevich Petr N.
Published: December 2021

Parameter identification is an important research topic with a variety of applications in industrial and environmental problems. Usually, a functional has to be minimized in conjunction with parameter identification; thus, there is a certain similarity between the parameter identification and optimization. A number of rigorous and efficient algorithms for optimization problems were developed in recent decades for the case of a convex functional. In the case of a non-convex functional, the metaheuristic algorithms dominate. This paper discusses an optimization method called modified bee colony algorithm (MBC), which is a modification of the standard bees algorithm (SBA). The SBA is inspired by a particular intelligent behavior of honeybee swarms. The algorithm is adapted for the parameter identification of reaction-dominated pore-scale transport when a non-convex functional has to be minimized. The algorithm is first checked by solving a few benchmark problems, namely finding the minima for Shekel, Rosenbrock, Himmelblau and Rastrigin functions. A statistical analysis was carried out to compare the performance of MBC with the SBA and the artificial bee colony (ABC) algorithm. Next, MBC is applied to identify the three parameters in the Langmuir isotherm, which is used to describe the considered reaction. Here, 2D periodic porous media were considered. The simulation results show that the MBC algorithm can be successfully used for identifying admissible sets for the reaction parameters in reaction-dominated transport characterized by low Pecklet and high Damkholer numbers. Finite element approximation in space and implicit time discretization are exploited to solve the direct problem.
Spatial-Temporal Variation of N, P, and K Stoichiometry in Cropland of Hainan Island
Authors: Ji Chunhua , Liu Hailin , Cha Zhengzao , Lin Qinghuo , Feng Gu
Published: December 2021

Elemental stoichiometry reflects the interaction between plants, soil, and microorganisms, and links biogeochemical patterns with physiological limitations. The stoichiometry of elements in farmland soil is an important part of the function of the agroecosystem. Soil nitrogen (N), phosphorus (P), and potassium (K) are the main macronutrients in terrestrial ecosystems, which are closely related to biogeochemical cycles. Studying the temporal and spatial variability of soil nutrients in tropical farmland is of great significance for exploring the variation of soil nutrients and promoting the sustainable development of tropical agriculture. In this study, soil samples in the farmland of Hainan Island were collected at three different stages for exploring temporal and spatial variations of N, P, and K stoichiometry. Results showed that soil concentrations of available N, P, and K changed markedly with the temporal and spatial variability. The highest available N, P, and K concentrations appeared at the stage of 2016–2020 with values of 110.40 mg/kg, 51.91 mg/kg, and 82.76 mg/kg, respectively, while their lowest values were observed in 2010–2015 with 66.34 mg/kg, 11.27 mg/kg, and 45.77 mg/kg, respectively. The available nitrogen content in the three time periods first increased and then decreased with the increase of available potassium content, an opposite trend was observed between available nitrogen and phosphorus. The content of N increased in Haikou, Lingao, Ding’an, and P increased in Wengchang, and Lingshui and K increased in Danzhou and Wanning as time increased.
The Effect of the Application of Stimulants on the Photosynthetic Apparatus and the Yield of Winter Wheat
Authors: Kraus Kamil , Hnilickova Helena , Pecka Jan , Lhotska Marie , Bezdickova Alena , Martinek Petr , Kucirkova Lenka , Hnilicka Frantisek
Published: December 2021

The use of stimulation preparations seems to be a promising means for mitigating the effects of abiotic and biotic stressors. Their significance includes plant organism stimulation and metabolism optimisation, water regime, and nutrition during periods of stress. They help bridge it over and create conditions for rapid regeneration. In a field experiment, the effect of the application of stimulation preparations on cultivars Triticum aestivum L. with different genetic composition was evaluated (donor of blue aleurone colour KM-72-18; donor of a multi-row spike (MRS) KM-94-18). Our results show a predominantly positive effect of the application of stimulants on the yield and thousand-grain weight (TKW). The results obtained were influenced by the year, based on different temperatures and precipitation. Higher yields were achieved in 2020 with higher total precipitation during the grain filling period and with a higher maximum quantum yield of the photosystem II (Fv/Fm). In 2019, this period was significantly dry and warm, which was reflected in a lower yield and TKM, higher proline content in the leaves, and lower Fv/Fm values. In both experimental years, there was a higher yield of the cultivar with blue aleurone (KM-72-18). In the case of cultivars with coloured grains, the promising use of the content substances in cultivars as natural means of increasing resistance to abiotic and biotic stressors seems to be promising.
A Comparison of Drill and Broadcast Planting Methods for Biomass Production of Two Legume Cover Crops
Authors: St Aime Ricardo , Noh Enoch , Bridges William C. , Narayanan Sruthi
Published: December 2021

The effects of drill and broadcast planting methods on cover crop biomass production depend on various environmental and operational factors. We investigated whether drilling and broadcasting result in different amounts of biomass production by crimson clover (Trifolium incarnatum L.) and hairy vetch (Vicia villosa Roth) in the upstate of South Carolina, and results vary when seeding rates are increased by 50% from the standard value (22.4 kg ha−1). Field trials were conducted during the fall–winter of 2019–2020 (season one) and 2020–2021 (season two) at the Piedmont Research and Education Center in Pendleton, SC, USA. Cover crop (hairy vetch, crimson clover), planting method (broadcast, drill), and seeding rate (standard, high) treatments were arranged as a 2 × 2 × 2 factorial in both years. Aboveground biomass was measured after 22.5 weeks from planting. At standard seeding rates, crimson clover produced a higher biomass when drilled, rather than broadcasted, whereas biomass production did not vary for hairy vetch. Even with 50% higher seeding rates, broadcasting did not always produce the same biomass as that of drilling for crimson clover. Our results suggest that the advantage of drilling over broadcasting depends upon the cover crop species, as crimson clover responds well to drilling, whereas hairy vetch does not.
Pilot Cultivation of the Local Endemic Cretan Marjoram Origanum microphyllum (Benth.) Vogel (Lamiaceae): Effect of Fertilizers on Growth and Herbal Quality Features
Authors: Fanourakis Dimitrios , Paschalidis Konstantinos , Tsaniklidis Georgios , Tzanakakis Vasileios A. , Bilias Fotis , Samara Eftihia , Liapaki Eleftheria , Jouini Manal , Ipsilantis Ioannis , Maloupa Eleni , Tsoktouridis Georgios , Matsi Theodora , Krigas Nikos
Published: December 2021

Wild phytogenetic resources are threatened by overexploitation. This pressure on species and natural ecosystems can be alleviated in part by recruiting and domesticating wild-growing species under pilot cultivation with tailor-made fertilization schemes. This study focused on the pilot cultivation of Origanum microphyllum—a critically endangered local endemic plant of Crete, Greece—investigating the effect of conventional and integrated nutrient management (ΙΝΜ) fertilizers by foliar or root application and biostimulant. Above-ground biomass together with leaf chlorophyll fluorescence and color (SPAD meter, DA meter, Chroma Meter) were determined. Leaf chlorophyll, antioxidant compound (carotenoids, phenols, flavonoids), and nutrient contents were also assessed. The results showed that fertilization did not significantly affect plant growth and leaf nutrient content. Root fertilization was associated with greener leaves compared to foliar. The same trend was generally evident for antioxidant compound content. The small size of leaves may have impeded the efficiency of the foliar application. In conclusion, root application of conventional or INM fertilizers seems more suitable to promote visual quality and herbal antioxidant profile of O. microphyllum, than the foliar one.
Measurement of Plasma Resistin Concentrations in Horses with Metabolic and Inflammatory Disorders
Authors: Fuentes-Romero Beatriz , Muñoz-Prieto Alberto , Cerón José J. , Martín-Cuervo María , Iglesias-García Manuel , Aguilera-Tejero Escolástico , Díez-Castro Elisa
Published: December 2021

Obesity and its associated complications, such as metabolic syndrome, are an increasing problem in both humans and horses in the developed world. The expression patterns of resistin differ considerably between species. In rodents, resistin is expressed by adipocytes and is related to obesity and ID. In humans, resistin is predominantly produced by inflammatory cells, and resistin concentrations do not reflect the degree of obesity, although they may predict cardiovascular outcomes. The aim of this study was to investigate the usefulness of resistin and its relationship with ID and selected indicators of inflammation in horses. Seventy-two horses, included in one of the four following groups, were studied: healthy controls (C, n = 14), horses with inflammatory conditions (I, n = 21), horses with mild ID (ID1, n = 18), and horses with severe ID (ID2, n = 19). Plasma resistin concentrations were significantly different between groups and the higher values were recorded in the I and ID2 groups (C: 2.38 ± 1.69 ng/mL; I: 6.85 ± 8.38 ng/mL; ID1: 2.41 ± 2.70 ng/mL; ID2: 4.49 ± 3.08 ng/mL). Plasma resistin was not correlated with basal insulin concentrations. A significant (r = 0.336, p = 0.002) correlation was found between resistin and serum amyloid A. Our results show that, as is the case in humans, plasma resistin concentrations in horses are predominantly related to inflammatory conditions and not to ID. Horses with severe ID showed an elevation in resistin that may be secondary to the inflammatory status associated with metabolic syndrome.
Identification of Monoallelically Expressed Genes Associated with Economic Traits in Hanwoo (Korean Native Cattle)
Authors: Lim Kyu-Sang , Kim Hyung-Chul , Choi Bong-Hwan , Son Ju-Whan , Lee Kyung-Tai , Choi Tae-Jeong , Cho Yong-Min , Chai Han-Ha , Park Jong-Eun , Park Woncheoul , Lim Chiwoong , Kim Jun-Mo , Lim Dajeong
Published: December 2021

Hanwoo, an indigenous Korean cattle breed, has been genetically improved by selecting superior sires called Korean-proven bulls. However, cows still contribute half of the genetic stock of their offspring, and allelic-specific expressed genes have potential, as selective targets of cows, to enhance genetic gain. The aim of this study is to identify genes that have MAEs based on both the genome and transcriptome and to estimate their effects on breeding values (BVs) for economically important traits in Hanwoo. We generated resequencing data for the parents and RNA-sequencing data for the muscle, fat, and brain tissues of the offspring. A total of 3801 heterozygous single nucleotide polymorphisms (SNPs) in offspring were identified and they were located in 1569 genes. Only 14 genes showed MAE (seven expressing maternal alleles and seven expressing paternal alleles). Tissue-specific MAE was observed, and LANCL1 showed maternal allele expression across all tissues. MAE genes were enriched for the biological process of cell death and angiogenesis, which included ACKR3 and PDCL3 genes, whose SNPs were significantly associated with BVs of lean meat production-related traits, such as weight at 12 months of age, carcass weight, and loin eye area. In the current study, monoallelically expressed genes were identified in various adult tissues and these genes were associated with genetic capacity in Hanwoo.
Antibiotic Prophylaxis for Surgical Site Infection in General Surgery: Oncological Treatments and HIPEC
Authors: Vallicelli Carlo , Coccolini Federico , Sartelli Massimo , Ansaloni Luca , Bui Simona , Catena Fausto
Published: December 2021

The procedure of cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) is a combined surgical and oncological treatment for peritoneal carcinomatosis of various origins. Antibiotic prophylaxis is usually center-related and should be discussed together with the infectious disease specialist, taking into account the advanced oncologic condition of the patient, the complexity of surgery—often requiring multiorgan resections—and the risk of post-HIPEC neutropenia. The incidence of surgical site infection (SSI) after CRS and HIPEC ranges between 11 and 46%. These patients are also at high risk of postoperative abdominal infections and septic complications, and a bacterial translocation during HIPEC has been hypothesized. Many authors have proposed aggressive screening protocols and a high intra and postoperative alert, in order to minimize and promptly identify all possible infectious complications following CRS and HIPEC.
The Associations between Poor Antibiotic and Antimicrobial Resistance Knowledge and Inappropriate Antibiotic Use in the General Population Are Modified by Age
Authors: Guo Huiling , Hildon Zoe Jane-Lara , Lye David Chien Boon , Straughan Paulin Tay , Chow Angela
Published: December 2021

Objectives: Understanding factors influencing inappropriate antibiotic use can guide the design of interventions to improve antibiotic practices and reduce antimicrobial resistance (AMR). Methods: A nationally representative cross-sectional survey (N = 2004) was conducted between November 2020 and January 2021. Knowledge of antibiotic use and AMR using the World Health Organization’s Multi-Country AMR Survey questionnaire, and antibiotic practices were examined. Multivariable logistic regression was performed to identify factors associated with inappropriate antibiotic use and examine effect measure modifications. Results: After adjusting for potential confounding, poor knowledge of antibiotic use was associated with a 3x increased odds of inappropriate antibiotic use in adults aged ≥50 years (aOR 3.11, 95% CI [2.24–4.32]), 5× increased odds in those aged 35–49 years (aOR 4.88, 95% CI [3.32–7.16]), and 7× increased odds in those aged 21–34 years (aOR 6.58, 95% CI [4.19–10.33]). While there was no statistically significant association in adults aged ≥50 years, poor knowledge of AMR increased the odds of inappropriate antibiotic use by 4 times in adults aged 35–49 years (aOR 3.73, 95% CI [1.53–9.11]) and 5 times in those aged 21–34 years (aOR 4.90, 95% CI [1.84–13.02]). Conclusions: Targeted educational interventions for specific age groups are needed in conjunction with empowering the public with knowledge of antibiotic use and AMR.
Trimethylamine N-Oxide Promotes Autoimmunity and a Loss of Vascular Function in Toll-like Receptor 7-Driven Lupus Mice
Authors: González-Correa Cristina , Moleón Javier , Miñano Sofía , Visitación Néstor de la , Robles-Vera Iñaki , Gómez-Guzmán Manuel , Jiménez Rosario , Romero Miguel , Duarte Juan
Published: December 2021

Plasma levels of trimethylamine N-oxide (TMAO) are elevated in lupus patients. We analyzed the implication of TMAO in autoimmunity and vascular dysfunction of the murine model of systemic lupus erythematosus (SLE) induced by the activation of the Toll-like receptor (TLR)7 with imiquimod (IMQ). Female BALB/c mice were randomly divided into four groups: untreated control mice, control mice treated with the trimethylamine lyase inhibitor 3,3-dimethyl-1-butanol (DMB), IMQ mice, and IMQ mice treated with DMB. The DMB-treated groups were administered the substance in their drinking water for 8 weeks. Treatment with DMB reduced plasma levels of TMAO in mice with IMQ-induced lupus. DMB prevents the development of hypertension, reduces disease progression (plasma levels of anti-dsDNA autoantibodies, splenomegaly, and proteinuria), reduces polarization of T lymphocytes towards Th17/Th1 in secondary lymph organs, and improves endothelial function in mice with IMQ-induced lupus. The deleterious vascular effects caused by TMAO appear to be associated with an increase in vascular oxidative stress generated by increased NADPH oxidase activity, derived in part from the vascular infiltration of Th17/Th1 lymphocytes, and reduced nrf2-driven antioxidant defense. In conclusion, our findings identified the bacterial-derived TMAO as a regulator of immune system, allowing for the development of autoimmunity and endothelial dysfunction in SLE mice.
Allicin, an Antioxidant and Neuroprotective Agent, Ameliorates Cognitive Impairment
Authors: Nadeem Muhammad Shahid , Kazmi Imran , Ullah Inam , Muhammad Khushi , Anwar Firoz
Published: December 2021

Allicin (diallylthiosulfinate) is a defense molecule produced by cellular contents of garlic (Allium sativum L.). On tissue damage, the non-proteinogenic amino acid alliin (S-allylcysteine sulfoxide) is converted to allicin in an enzyme-mediated process catalysed by alliinase. Allicin is hydrophobic in nature, can efficiently cross the cellular membranes and behaves as a reactive sulfur species (RSS) inside the cells. It is physiologically active molecule with the ability to oxidise the thiol groups of glutathione and between cysteine residues in proteins. Allicin has shown anticancer, antimicrobial, antioxidant properties and also serves as an efficient therapeutic agent against cardiovascular diseases. In this context, the present review describes allicin as an antioxidant, and neuroprotective molecule that can ameliorate the cognitive abilities in case of neurodegenerative and neuropsychological disorders. As an antioxidant, allicin fights the reactive oxygen species (ROS) by downregulation of NOX (NADPH oxidizing) enzymes, it can directly interact to reduce the cellular levels of different types of ROS produced by a variety of peroxidases. Most of the neuroprotective actions of allicin are mediated via redox-dependent pathways. Allicin inhibits neuroinflammation by suppressing the ROS production, inhibition of TLR4/MyD88/NF-κB, P38 and JNK pathways. As an inhibitor of cholinesterase and (AChE) and butyrylcholinesterase (BuChE) it can be applied to manage the Alzheimer’s disease, helps to maintain the balance of neurotransmitters in case of autism spectrum disorder (ASD) and attention deficit hyperactive syndrome (ADHD). In case of acute traumatic spinal cord injury (SCI) allicin protects neuron damage by regulating inflammation, apoptosis and promoting the expression levels of Nrf2 (nuclear factor erythroid 2-related factor 2). Metal induced neurodegeneration can also be attenuated and cognitive abilities of patients suffering from neurological diseases can be ameliorates by allicin administration.
Toxicity of Antioxidant Supplements in Patients with Male Factor Infertility: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Creta Massimiliano , Arcaniolo Davide , Celentano Giuseppe , Napolitano Luigi , La Rocca Roberto , Capece Marco , Califano Gianluigi , Mangiapia Francesco , Spirito Lorenzo , Crocetto Felice , Imbimbo Ciro , Longo Nicola , De Sio Marco , Fusco Ferdinando
Published: December 2021

Treating oxidative stress through antioxidant therapy has been considered an appealing strategy in the management of male infertility. However, evidence regarding the toxicity of antioxidant therapy is controversial. We summarized the available clinical evidence on the toxicity associated with the use of antioxidants in infertile males. A systematic review was performed in March 2021. We included randomized controlled trials evaluating the incidence of adverse events in male patients with infertility receiving antioxidant therapy. Thirteen studies involving 1999 male patients were identified. Antioxidant supplementation in patients with male factor infertility was associated with a statistically significantly increased risk of nausea (Odds Ratio: 2.16, 95% Confidence Interval: 1.05–4.43, p = 0.036), headache (Odds Ratio: 3.05, 95% Confidence Interval: 1.59– 5.85 p = 0.001), and dyspepsia (Odds Ratio: 4.12, 95% Confidence Interval: 1.43–11.85, p = 0.009) compared to a placebo. Treatment discontinuation due to adverse events was not significantly higher in patients taking antioxidants compared to a placebo (Odds Ratio: 2.29, 95% Confidence Interval: 0.76–6.88, p = 0.139). When antioxidant supplementation is considered, a more accurate risk/benefit analysis is warranted.
Physical Activity vs. Redox Balance in the Brain: Brain Health, Aging and Diseases
Authors: Sutkowy Paweł , Woźniak Alina , Mila-Kierzenkowska Celestyna , Szewczyk-Golec Karolina , Wesołowski Roland , Pawłowska Marta , Nuszkiewicz Jarosław
Published: December 2021

It has been proven that physical exercise improves cognitive function and memory, has an analgesic and antidepressant effect, and delays the aging of the brain and the development of diseases, including neurodegenerative disorders. There are even attempts to use physical activity in the treatment of mental diseases. The course of most diseases is strictly associated with oxidative stress, which can be prevented or alleviated with regular exercise. It has been proven that physical exercise helps to maintain the oxidant–antioxidant balance. In this review, we present the current knowledge on redox balance in the organism and the consequences of its disruption, while focusing mainly on the brain. Furthermore, we discuss the impact of physical activity on aging and brain diseases, and present current recommendations and directions for further research in this area.
Concentration of Antioxidant Compounds from Calendula officinalis through Sustainable Supercritical Technologies, and Computational Study of Their Permeability in Skin for Cosmetic Use
Authors: Mur Raquel , Langa Elisa , Pino-Otín M. Rosa , Urieta José S. , Mainar Ana M.
Published: December 2021

The growing interest in the cosmetic industry in using compounds of natural and sustainable origin that are safe for humans is encouraging the development of processes that can satisfy these needs. Chlorogenic acid (CHA), caffeic acid (CAF) and ferulic acid (FA) are three compounds widely used within the cosmetic industry due to their functionalities as antioxidants, collagen modifiers or even as radiation protectors. In this work, two advanced separation techniques with supercritical CO2 are used to obtain these three compounds from Calendula officinalis, and these are then evaluated using a computational skin permeability model. This model is encompassed by the COSMO-RS model, the calculations of which make it possible to study the behaviour of the compounds in the epidermis. The results show that both CAF and FA are retained in the stratum corneum, while CHA manages to penetrate to the stratum spinosum. These compounds were concentrated by antisolvent fractionation with super-critical CO2 using a Response Surface Methodology to study the effect of pressure and CO2 flow rate. CHA, CAF and FA were completely retained in the precipitation vessel, with concentrations between 40% and 70% greater than in the original extract. The conditions predicted that the optimal overall yield and enrichment achieved would be 153 bar and 42 g/min.
Role of Nrf2 in Pancreatic Cancer
Authors: Cykowiak Marta , Krajka-Kuźniak Violetta
Published: December 2021

Pancreatic tumors are a serious health problem with a 7% mortality rate worldwide. Inflammatory processes and oxidative stress play important roles in the development of pancreatic diseases/cancer. To maintain homeostasis, a balance between free radicals and the antioxidant system is essential. Nuclear Factor Erythroid 2-Related Factor 2/NFE2L2 (Nrf2) and its negative regulator Kelch-Like ECH-Associated Protein 1 (Keap1) provide substantial protection against damage induced by oxidative stress, and a growing body of evidence points to the canonical and noncanonical Nrf2 signaling pathway as a pharmacological target in the treatment of pancreatic diseases. In this review, we present updated evidence on the activation of the Nrf2 signaling pathway and its importance in pancreatic cancer. Our review covers potential modulators of canonical and noncanonical pathway modulation mechanisms that may have a positive effect on the therapeutic response. Finally, we describe some interesting recent discoveries of novel treatments related to the antioxidant system for pancreatic cancer, including natural or synthetic compounds with therapeutic properties.
Prunus cerasoides Extract and Its Component Compounds Upregulate Neuronal Neuroglobin Levels, Mediate Antioxidant Effects, and Ameliorate Functional Losses in the Mouse Model of Cerebral Ischemia
Authors: Kim So-Dam , Kim Minha , Wu Hong-Hua , Jin Byung Kwan , Jeon Myung-Shin , Song Yun Seon
Published: December 2021

Prunus cerasoides (PC) has been reported to have antimicrobial and anti-inflammatory properties, but its potential as a neuroprotective agent in a mouse model of cerebral ischemia has not been explored. Considering neuroglobin (Ngb), an endogenous neuroprotective factor, as a novel approach to neuroprotection, in this study, Ngb promoter activity, Ngb expression changes, and antioxidant protection by PC extract (PCE) and PC component compounds (PCCs) were analyzed in oxygen–glucose deprivation (OGD)-treated neurons. In vivo analysis involved transient middle cerebral artery occlusion (tMCAO) in mice with pre- and post-treatment exposure to PCE. Following ischemic stroke induction, neurological behavior scores were obtained, and cellular function-related signals were evaluated in the ischemic infarct areas. In addition to PCE, certain component compounds from PCE also significantly increased Ngb levels and attenuated the intracellular ROS production and cytotoxicity seen with OGD in primary neurons. Administration of PCE reduced the infarct volume and improved neurological deficit scores in ischemic stroke mice compared with the vehicle treatment. Increased Ngb levels in infarct penumbra with PCE treatment were also accompanied by decreased markers of apoptosis (activated p38 and cleaved caspase-3). Our findings point to the benefits of Ngb-mediated neuroprotection via PCE and its antioxidant activity in an ischemic stroke model.
Optimized Cell Mixing Facilitates the Reproducible Bioprinting of Constructs with High Cell Viability
Authors: Pössl Axel , Hartzke David , Schlupp Peggy , Runkel Frank E.
Published: December 2021

Bioprinting with cell-laden hydrogels (bioink) requires the careful mixing of cells with the hydrogel carrier to ensure that the bioink is homogeneous and functional, and the printing results are reproducible. Bioink preparation is therefore a critical process step that must accommodate the specific rheological properties of different bioinks. Here, we developed a reproducible method for the optimized mixing of cells and hydrogel carriers that can be integrated into current bioprinting processes. First, we tested and optimized different mixing devices for their effect on bioink homogeneity and rheological properties, resulting in a low-shear process for the preparation of homogenous bioinks. Based on these findings, we evaluated the impact of different cell densities on the rheological profile of bioinks according to shear and temperature, and estimated the impact of shear stress intensity and duration on 1.1B4 cells. Finally, we integrated the optimized mixing method into a current printing process and monitored the printed construct for 14 days to confirm cell viability. We found that the cell viability in the printed cell-laden constructs remained in excess of 91% after 14 days.
Development of a Pre-Evaluation and Health Monitoring System for FAST Cable-Net Structure
Authors: Shen Yuzhou , Luo Bin , Jiang Peng , Ding Mingmin , Li Qingwei , Wei Yang
Published: December 2021

The Five-hundred-meter Aperture Spherical radio Telescope (FAST) is one of China’s major pieces of national infrastructure. A variable cable-net structure is used as the main supporting structure of the active reflector. The displacement of the cable net works through actuators. The realization of linkage control is a multi-degree-of-freedom and complex coupling control system. Due to factors such as the temperature difference between day and night, as well as actuator failure, the reflector control accuracy and even structural safety are affected during the position-control process of the cable net, so realizing evaluation of control accuracy and fault warning of the reflector is a significant problem. This paper proposes a pre-evaluation and health monitoring system based on advanced mechanical simulation technology. Through this system, on-site staff can expeditiously analyze the model to determine whether the cable net is currently in a safe state, predict the fatigue degree of the components, and maintain the structure when appropriate. The pre-evaluation and health monitoring system adequately ensure the stable functioning of the FAST cable net, improve the efficiency of on-site maintenance work, and markedly reduce the safety risk of the structure.
Design and Testing of Two Haptic Devices Based on Reconfigurable 2R Joints
Authors: Riabtsev Mykhailo , Petuya Victor , Urízar Mónica , Altuzarra Oscar
Published: December 2021

This paper presents the design and testing of two haptic devices, based on reconfigurable 2R joints: an active 2R spherical mechanism-based joint and a differential gear-based joint. Based on our previous works, in which the design and kinematic analysis of both reconfigurable joints were developed, the experimental setup and the various tasks intended to test the reconfigurability, precision, force feedback system and general performance, are presented herein. Two control modes for the haptic device operation are proposed and studied. The statistical analysis tools and their selection principles are described. The mechanical design of two experimental setups and the main elements are considered in detail. The Robot Operating System nodes and the topics that are used in the software component of the experimental setup are presented and explained. The experimental testing was carried out with a number of participants and the corresponding results were analyzed with the selected statistical tools. A detailed interpretation and discussion on of the results is provided.
A Herbal Concoction of Cinnamomum cassia and Artemisa annua Extracts Ameliorates Allergic Rhinitis in OVA-Induced Balb/C Mice by Inhibiting Th2 Signaling
Authors: Bae Chuhyun , Kim Jisoo , Park Soodong , Shim Jaejung , Lee Junglyoul
Published: December 2021

Allergic rhinitis (AR) is an inflammatory airway disease (IAD) that is characterized by itching, nasal obstruction, and sneezing. AR is induced by Th-2 inflammatory responses such as those mediated by IgE and IL-4. This study aims to investigate the therapeutic effects of an herbal concoction, which is a combination of Cinnamomum cassia and Artemisa annua extracts (CIAR) against ovalbumin (OVA)-induced allergic rhinitis in a Balb/C mouse model. The effect of CIAR on the Th-2 mediated inflammatory response in the AR mouse model was studied by analyzing blood or nasal fluid samples. Experimental results revealed that OVA inhalation increased IgE, IL-4, IL-33, and TSLP levels, leading to Th2-type cytokine response. CIAR was found to significantly reduce the Th-2 response and levels of cytokines, including IL-4, IL-33, and thymic stromal lymphopoietin (TSLP). CIAR also down-regulated eosinophil (EOS) and basophil (BASO) levels in the blood. Histological analyses demonstrated decreased OVA-induced thickness of the respiratory epithelium in the CIAR-treated group. Collectively, our results suggest that the herbal concoction CIAR can effectively ameliorate the development of allergic rhinitis through the inhibition of Th-2 mediated responses.
Replacing Mineral Fertilisers for Bio-Based Fertilisers in Potato Growing on Sandy Soil: A Case Study
Authors: Hendriks Chantal M. J. , Shrivastava Vaibhav , Sigurnjak Ivona , Lesschen Jan Peter , Meers Erik , Noort Rembert van , Yang Zhongchen , Rietra Rene P. J. J.
Published: December 2021

The refinement level of bio-based fertilisers (BBFs) can influence environmental and agronomic performance. This study analyses the environmental and agronomic effect of different BBFs on potato growing in sandy soil. A less refined product (liquid fraction of digestate (LFD)), two refined products (ammonium sulphate (AS) and potassium concentrate (KC)), and mineral fertilizer (MF) are compared by conducting: (i) a nitrogen (N) incubation experiment where the N release rate of the BBFs is determined, (ii) a greenhouse gas emission experiment where N2O, CO2, and CH4 emissions after BBF application are measured, (iii) a pot experiment where the nutrient fertiliser replacement value (NFRV) of the BBF is calculated, and (iv) a full-scale field trial where the potato quality and quantity and the remaining N residues in the soil after harvest are assessed. The N release rate and the NFRV of AS (142 ± 19% and 1.13, respectively) was higher compared with the LFD (113 ± 24% and 1.04) and MF (105 ± 16% and 1.00). Lowest N2O emissions were observed after the application of the less refined product (0.02 ± 0.01 per 100 g N applied) and highest for MF urea (0.11 ± 0.02 per 100 g N applied). In the full-scale field trial, no significant difference in potato yield was observed in the plots that received manure in combination with BBF or MF. This study showed that all three BBFs can safely be used in potato growing on sandy soils. However, the adoption of BBFs can be stimulated by (i) solving the practical issues that occurred during the application of LFD, (ii) making sure BBFs are on the list of RENURE materials so they can legally replace mineral fertiliser, and (iii) reducing the surplus of slurry manure to stimulate the use and fair pricing of BBF products.
Railway Wireless Communications Channel Characterization
Authors: Fernández-Berrueta Nerea , Goya Jon , Arrizabalaga Jaione , Moya Iker , Mendizabal Jaizki
Published: December 2021

Railway applications are in continuous evolution with the aim of offering a more efficient, sustainable, and safer transportation system for the users. Generally, these applications are constantly exchanging information between the systems onboard the train and the trackside through a wireless communication. Nowadays, Global System for Mobile communications-Railway (GSM-R) is the technology used by European Train Control System (ETCS), but it is becoming obsolete. Therefore, alternatives for this technology have to be found for the different railway applications. Its natural evolution is to move forward with the latest technology deployed: Long-Term Evolution (LTE), which the Public Land Mobile Networks (PLMN) have already deployed. Therefore, testing the performance of this communication technology in the railway environment could be useful to assess its suitability and reduce the cost of railway network dedicated deployment. In order to do that, a methodology to characterize the communication environment is proposed. The main goal is to measure geolocated impairments of any communication channel in a railway environment being able to determine its behavior of the different communication technologies and find out possible coverage issues. Moreover, it could help in the selection of suitable communication technology for railway. This paper presents a brief description of the communication for railways and its QoS parameters for performance measuring. Afterward, the testing methodology is described, and then, the communication channel measurement campaign on a real track in Spain where the railway environment is variable is presented (tunnels, rural/urban area…). Finally, the measurements and results on this real track in Spain are shown. The results provide suitability of the 4G technologies based on the delay requirements for the implementation of ETCS over it.
Digital Twins, Virtual Devices, and Augmentations for Self-Organising Cyber-Physical Collectives
Authors: Casadei Roberto , Pianini Danilo , Viroli Mirko , Weyns Danny
Published: December 2021

The engineering of large-scale cyber-physical systems (CPS) increasingly relies on principles from self-organisation and collective computing, enabling these systems to cooperate and adapt in dynamic environments. CPS engineering also often leverages digital twins that provide synchronised logical counterparts of physical entities. In contrast, sensor networks rely on the different but related concept of virtual device that provides an abstraction of a group of sensors. In this work, we study how such concepts can contribute to the engineering of self-organising CPSs. To that end, we analyse the concepts and devise modelling constructs, distinguishing between identity correspondence and execution relationships. Based on this analysis, we then contribute to the novel concept of “collective digital twin” (CDT) that captures the logical counterpart of a collection of physical devices. A CDT can also be “augmented” with purely virtual devices, which may be exploited to steer the self-organisation process of the CDT and its physical counterpart. We underpin the novel concept with experiments in the context of the pulverisation framework of aggregate computing, showing how augmented CDTs provide a holistic, modular, and cyber-physically integrated system view that can foster the engineering of self-organising CPSs.
Data Augmentation Based on Generative Adversarial Networks to Improve Stage Classification of Chronic Kidney Disease
Authors: Liao Yun-Te , Lee Chien-Hung , Chen Kuo-Su , Chen Chie-Pein , Pai Tun-Wen
Published: December 2021

The prevalence of chronic kidney disease (CKD) is estimated to be 13.4% worldwide and 15% in the United States. CKD has been recognized as a leading public health problem worldwide. Unfortunately, as many as 90% of CKD patients do not know that they already have CKD. Ultrasonography is usually the first and the most commonly used imaging diagnostic tool for patients at risk of CKD. To provide a consistent assessment of the stage classifications of CKD, this study proposes an auxiliary diagnosis system based on deep learning approaches for renal ultrasound images. The system uses the ACWGAN-GP model and MobileNetV2 pre-training model. The images generated by the ACWGAN-GP generation model and the original images are simultaneously input into the pre-training model MobileNetV2 for training. This classification system achieved an accuracy of 81.9% in the four stages of CKD classification. If the prediction results allowed a higher stage tolerance, the accuracy could be improved by up to 90.1%. The proposed deep learning method solves the problem of imbalance and insufficient data samples during training processes for an automatic classification system and also improves the prediction accuracy of CKD stage diagnosis.
Integrated Intelligent CAD System for Interactive Design, Analysis and Prototyping of Compression and Torsion Springs
Authors: Saric Isad , Muratovic Enis , Muminovic Adil , Muminovic Adis J. , Colic Mirsad , Delic Muamer , Pervan Nedim , Mesic Elmedin
Published: December 2021

This paper presents the development and implementation of integrated intelligent CAD (computer aided design) system for design, analysis and prototyping of the compression and torsion springs. The article shows a structure of the developed system named Springs IICAD (integrated intelligent computer aided design). The system bounds synthesis and analysis design phases by means of the utilization of parametric 3D (three-dimensional) modeling, FEM (finite element method) analysis and prototyping. The development of the module for spring calculation and system integration was performed in the C# (C Sharp) programming language. Three-dimensional geometric modeling and structural analysis were performed in the CATIA (computer aided three-dimensional interactive application) software, while prototyping is performed with the Ultimaker 3.0 3D printer with support of Cura software. The developed Springs IICAD system interlinks computation module with the basic parametric models in such a way that spring calculation, shaping, FEM analysis and prototype preparation are performed instantly.
Application of the Supercapacitor for Energy Storage in China: Role and Strategy
Authors: Yang Yanchun , Han Yinghui , Jiang Wenkun , Zhang Yuyang , Xu Yanmei , Ahmed Ayman Mahmoud
Published: December 2021

Supercapacitors are widely used in China due to their high energy storage efficiency, long cycle life, high power density and low maintenance cost. This review compares the differences of different types of supercapacitors and the developing trend of electrochemical hybrid energy storage technology. It gives an overview of the application status of supercapacitors in China’s smart grid and Energy Internet in detail. Some strategies and constructive suggestions are put forward to solve the existing problems. With the improvement of the grid-connected capacity of new energy power generation during the 14th Five-year Period of China, the supercapacitor market in China will usher in a good development opportunity. The role of the supercapacitor in achieving carbon peak carbon neutralization is prospected.
Foot Insole Pressure Distribution during the Golf Swing in Professionals and Amateur Players
Authors: Navarro Enrique , Mancebo José M. , Farazi Sima , del Olmo Malena , Luengo David
Published: December 2021

There are numerous articles that study the ground reaction forces during the golf swing, among which only a few analyze the pressure pattern distributed on the entire surface of the foot. The current study compares the pressure patterns on the foot insoles of fifty-five golfers, from three different performance levels, playing swings with driver and 5-iron clubs in the driving range. Five swings were selected for each club. During each swing, ultra-thin insole sensors (4 sensors/cm2) measure foot pressure at the frequency of 100 Hz. To perform statistical analysis, insole sensors are clustered to form seven areas, with the normalized pressure of each area being our dependent variable. A video camera was used to label the five key instants of the swing. Statistical analysis demonstrates a significant difference between the pressure distribution pattern of the left and right feet for both driver and 5-iron. However, the pressure distribution pattern remains almost the same when switching the club type from 5-iron to driver. We have also observed that there are significant differences between the pattern of professionals and players with medium and high handicap. The obtained pattern agrees with the principle of weight transfer with a different behavior between the medial and lateral areas of the foot.
Machine-Learning-Based Coefficient of Performance Prediction Model for Heat Pump Systems
Authors: Shin Ji-Hyun , Cho Young-Hum
Published: December 2021

In a heat pump system, performance is an important indicator that should be monitored for system optimization, fault diagnosis, and operational efficiency improvement. Real-time performance measurement and monitoring during heat pump operation is difficult because expensive performance measurement devices or additional installation of various monitoring sensors required for performance calculation are required. When using a data-based machine-learning model, it is possible to predict and monitor performance by finding the relationship between input and output values through an existing sensor. In this study, the performance prediction model of the air-cooled heat pump system was developed and verified using artificial neural network, support vector machine, random forest, and K-nearest neighbor model. The operation data of the heat pump system installed in the university laboratory was measured and a prediction model for each machine-learning stage was developed. The mean bias error analysis is −3.6 for artificial neural network, −5 for artificial neural network, −7.7 for random forest, and −8.3 for K-nearest neighbor. The artificial neural network model with the highest accuracy and the shortest calculation time among the developed prediction models was applied to the Building Automation System to enable real-time performance monitoring and to confirm the field applicability of the developed model.
Emerging Hydro-Climatic Patterns, Teleconnections, and Extreme Events in Changing World at Different Timescales
Authors: Agarwal Ankit , Yuan Naiming , Cheung Kevin K. W. , Shukla Roopam
Published: December 2021

The Atmosphere Special Issue, entitled “Emerging Hydro-Climatic Patterns, Teleconnections and Extreme Events in Changing World at Different Timescales”, comprises thirteen original papers [...]
Characteristics of Precipitation Diurnal Cycle over a Mountainous Area of Sumatra Island including MJO and Seasonal Signatures Based on the 15-Year Optical Rain Gauge Data, WRF Model and IMERG
Authors: Marzuki Marzuki , Yusnaini Helmi , Ramadhan Ravidho , Tangang Fredolin , Amirudin Abdul Azim Bin , Hashiguchi Hiroyuki , Shimomai Toyoshi , Vonnisa Mutya
Published: December 2021

In this study we investigate the characteristics of the diurnal precipitation cycle including the Madden–Julian oscillation (MJO) and seasonal influences over a mountainous area in Sumatra Island based on the in situ measurement of precipitation using the optical rain gauge (ORG). For comparison with ORG data, the characteristics based on the Global Precipitation Measurement (GPM) mission (IMERG) and Weather Research and Forecasting (WRF) simulations were also investigated. Fifteen years of ORG data over a mountainous area of Sumatra, namely, at Kototabang (100.32° E, 0.20° S), were analyzed to obtain the characteristics of the diurnal cycle of precipitation in this region. The diurnal cycle of precipitation presented a single peak in the late afternoon, and the peak time difference was closely related to the rain event duration. The MJO acts to modulate the diurnal amplitude but not the diurnal phase. A high precipitation amount (PA) and frequency (PF) were observed during phases 2, 3, and 4, along with an increase in the number of longer-duration rain events, but the diurnal phase was similar in all MJO phases. In terms of season, the highest PA and PF values were observed during pre-southwest and pre-northeast monsoon seasons. WRF simulation reproduced the diurnal phase correctly and more realistically than the IMERG products. However, it largely overestimated the amplitude of the diurnal cycle in comparison with ORG. These disagreements could be related to the resolution and quality of IMERG and WRF data.
Peptide Multimerization as Leads for Therapeutic Development
Authors: Sheard Dean E. , Li Wenyi , O’Brien-Simpson Neil M. , Separovic Frances , Wade John D.
Published: December 2021

Multimerization of peptide structures has been a logical evolution in their development as potential therapeutic molecules. The multivalent properties of these assemblies have attracted much attention from researchers in the past and the development of more complex branching dendrimeric structures, with a wide array of biocompatible building blocks is revealing previously unseen properties and activities. These branching multimer and dendrimer structures can induce greater effect on cellular targets than monomeric forms and act as potent antimicrobials, potential vaccine alternatives and promising candidates in biomedical imaging and drug delivery applications. This review aims to outline the chemical synthetic innovations for the development of these highly complex structures and highlight the extensive capabilities of these molecules to rival those of natural biomolecules.