bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-designing Science Lessons in Spanish: Connecting Science, Home Language, and Community for Undergraduates
Authors: Nation Jasmine McBeath , Yep Alejandra
Published: August 2024

Nuestra Ciencia (NC) is a university-school partnership connecting faculty and undergraduates with elementary learners from bilingual schools. Interdisciplinary teams of undergraduates and faculty develop experiments and educational resources, and lead activities in Spanish. The program teaches elementary students everyday microbiology concepts, engages them in hands-on science, and connects them with Spanish-speaking scientific role models. This chapter describes the collaborative process of developing and teaching a virtual lesson in Spanish about vaccines’ mechanism of action. We analyze the impact of NC undergraduates’ views on research, scientific content in Spanish, and sense of belonging to STEM and higher education. We present how undergraduates came to view research as collaborative and relevant, as they reported bringing their “whole selves” into the design and teaching process, and saw the “real-world impact” of microbiology lessons that mattered to their communities. Additionally, designing and teaching in Spanish impacted their views on academic Spanish, as they connected science, home language, and community. Multiple goals of NC focus on Spanish-speaking children’s microbiology literacy and interest in science. However, the impact of our co-design process on undergraduates shows that, as we work on designing and implementing lessons, NC also has a positive impact on undergraduates and their families.
Making Connections: Pandemic-Era Lessons from a Maker-Centered University–Community Partnership
Authors: Martin Lee , Lee Jade , Leapman J. C. , Rainey-Ruiz Ann
Published: August 2024

Beta Lab Links connects UC Davis faculty and students with young people in the region to support them in imagining, designing, and building “maker” projects of their own choosing. The idea of “making” is broad and has a long history, but it has recently become associated with playful tinkering and design work that combines low-tech craft technologies (e.g., sewing, woodworking, and papercraft) with high-tech tools (e.g., microcontrollers, 3-D printers, software design). Advocates of maker-centered education emphasize its potential to foster STEM identity, knowledge, and skill development in a fun and inclusive environment.

Typically, Beta Lab Links work in person at the schools or community centers youth already attend, using a mobile maker van to create “pop-up” making spaces. During COVID-19 closures, Beta Lab Links collaborated with the Boys & Girls Club of Greater Sacramento to distribute maker kits to families and hold weekly online sessions with upper elementary-school-aged youth. These activities focused primarily on papercraft and electronics, as these were novel, STEM-rich, and did not require additional tools.

This chapter shares Beta Lab’s core pedagogical commitments and explores how the move online provided a catalyst for reflection and new insights into processes of connection and community through making.
Microstructural homogenization and mechanical enhancement of aluminum matrix composites via multi-pass friction stir processing with SiC reinforcements
Authors: Leszczyńska-Madej Beata , Madej Marcin , Wąsik Anna , Węglowska Aleksandra
Published: August 2024

In this study, the environmentally friendly friction stir processing (FSP) method was utilized to fabricate surface composites employing technical aluminum matrix 1050-H14 and aluminum alloy 6060-T4 reinforced with silicon carbide (SiC) particles. Microstructure analysis, employing light and scanning electron microscopy, in conjunction with comprehensive evaluations of hardness, compressive strength, and tribological properties, was conducted to elucidate significant findings. The results reveal that an augmented number of FSP passes contributes to the homogenization of microstructure, leading to the alteration of SiC particle morphology and fragmentation. Consequently, this phenomenon results in improved mechanical properties, particularly noteworthy in the case of AA6060-T4 alloy matrix composites, and enhanced wear resistance. Both AA1050-SiC and AA6060-SiC composites demonstrate notable increases in compressive strength compared to their unreinforced matrices. Particularly noteworthy is the substantial enhancement in compressive strength observed in the AA6060-SiCp composite, escalating from 249 to 331 MPa (at ε = 0.1) and from 398 to 715 MPa (at ε = 0.2) with an increase in the number of FSP passes. Additionally, FSP’s ability to precisely control process parameters such as tool rotational speed and traverse speed allows for the optimization of mechanical properties and microstructural characteristics tailored to specific application requirements. This study highlights the potential of FSP in fabricating high-performance aluminum matrix composites with superior strength and wear resistance, positioning it as a viable technique for advanced engineering applications. The environmentally friendly nature of FSP, due to its solid-state operation and reduced energy consumption, further underscores its suitability for sustainable manufacturing practices.
Metabolic activation of WHO-congeners PCB28, 52, and 101 by human CYP2A6: evidence from in vitro and in vivo experiments
Authors: Randerath Isabella , Schettgen Thomas , Müller Julian Peter , Rengelshausen Jens , Ziegler Susanne , Quinete Nathalia , Bertram Jens , Laieb Salah , Schaeffeler Elke , Kaifie Andrea , Just Katja S. , Voigt Aaron , Tremmel Roman , Schwab Matthias , Stingl Julia C. , Kraus Thomas , Ziegler Patrick
Published: August 2024

Despite extensive research on the metabolism of polychlorinated biphenyls (PCBs), knowledge gaps persist regarding their isoform-specific biotransformation pathways. This study aimed to elucidate the role of different cytochrome P450 enzymes in PCB metabolism, focusing on WHO-congeners 2,4,4′-trichlorobiphenyl (PCB28), 2,2′,5,5′-tetrachlorobiphenyl (PCB52), and 2,2′,4,5,5′-pentachlorobiphenyl (PCB101). Utilizing engineered HEK293 cell lines, we investigated the in vitro metabolism of these PCBs by CYP1A2, CYP2C8, CYP2C9, CYP3A4, CYP2A6, and CYP2E1, revealing robust production of hydroxylated metabolites. Our results show that CYP2A6 plays a major role in the metabolism of these congeners responsible for predominant formation of para-position hydroxylated metabolites, with concentrations reaching up to 1.61 µg/L (5,89 nM) for PCB28, 316.98 µg/L (1,03 µM) for PCB52, and 151.1 µg/L (441 nM) for PCB101 from a 20 µM parent PCB concentration. Moreover, concentration-dependent cytotoxic and cytostatic effects induced by reactive intermediates of the PCB hydroxylation pathway were observed in HEK293CYP2A6 cells, for all three congeners tested. CYP2A6 was specifically capable of activating PCBs 28 and 101 to genotoxic metabolites which produced genetic defects which were propagated to subsequent generations, potentially contributing to carcinogenesis. In a clinical study examining CYP2A6 enzyme activity in formerly exposed individuals with elevated internal PCB levels, a participant with increased enzyme activity showed a direct association between the phenotypic activity of CYP2A6 and the metabolism of PCB28, confirming the role of CYP2A6 in the in vivo metabolism of PCB28 also in humans. These results altogether reinforce the concept that CYP2A6 plays a pivotal role in PCB congener metabolism and suggest its significance in human health, particularly in the metabolism of lower chlorinated, volatile PCB congeners.
Deep learning-aided respiratory motion compensation in PET/CT: addressing motion induced resolution loss, attenuation correction artifacts and PET-CT misalignment
Authors: Lu Yihuan , Kang Fei , Zhang Duo , Li Yue , Liu Hao , Sun Chen , Zeng Hao , Shi Lei , Zhao Yumo , Wang Jing
Published: August 2024

Purpose

Respiratory motion (RM) significantly impacts image quality in thoracoabdominal PET/CT imaging. This study introduces a unified data-driven respiratory motion correction (uRMC) method, utilizing deep learning neural networks, to solve all the major issues caused by RM, i.e., PET resolution loss, attenuation correction artifacts, and PET-CT misalignment.

Methods

In a retrospective study, 737 patients underwent [18F]FDG PET/CT scans using the uMI Panorama PET/CT scanner. Ninety-nine patients, who also had respiration monitoring device (VSM), formed the validation set. The remaining data of the 638 patients were used to train neural networks used in the uRMC. The uRMC primarily consists of three key components: (1) data-driven respiratory signal extraction, (2) attenuation map generation, and (3) PET-CT alignment. SUV metrics were calculated within 906 lesions for three approaches, i.e., data-driven uRMC (proposed), VSM-based uRMC, and OSEM without motion correction (NMC). RM magnitude of major organs were estimated.

Results

uRMC enhanced diagnostic capabilities by revealing previously undetected lesions, sharpening lesion contours, increasing SUV values, and improving PET-CT alignment. Compared to NMC, uRMC showed increases of 10% and 17% in SUVmax and SUVmean across 906 lesions. Sub-group analysis showed significant SUV increases in small and medium-sized lesions with uRMC. Minor differences were found between VSM-based and data-driven uRMC methods, with the SUVmax was found statistically marginal significant or insignificant between the two methods. The study observed varied motion amplitudes in major organs, typically ranging from 10 to 20 mm.

Conclusion

A data-driven solution for respiratory motion in PET/CT has been developed, validated and evaluated. To the best of our knowledge, this is the first unified solution that compensates for the motion blur within PET, the attenuation mismatch artifacts caused by PET-CT misalignment, and the misalignment between PET and CT.
The prevalence and anatomy of accessory navicular bone: a meta-analysis
Authors: Stolarz Kacper , Osiowski Aleksander , Preinl Maciej , Osiowski Maksymilian , Jasiewicz Barbara , Taterra Dominik
Published: August 2024

Purpose

There have been over 40 descriptions of the common developmental variants of the accessory ossicles of the feet. Although predominantly asymptomatic, they sometimes may be linked to painful conditions. One of the most common accessory ossicles in the foot is the accessory navicular bone (AN), located on the medial side of the foot. Our research provides a first meta-analysis on this topic that establishes its frequency by contrasting 39 studies from across the globe.

Methods

Up to February 2024, PubMed and Embase databases were thoroughly searched for research on the AN. Eligible data regarding AN prevalence was extracted. This study strictly adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.

Results

A total of 39 studies, 11,015 patients, and 36,837 feet were analyzed in our study. The pooled prevalence estimate (PPE) of AN was found to be 17.5% (95%CI: 11.5–25.7) and 12.6% (95%CI: 10.1–15.5) in patients and feet analyses, respectively. Accessory navicular occurred bilaterally in 50.0% of patients, with similar distribution in gender-based groups (21.1% of males and 22.0% of females were confirmed with AN). Accessory navicular was most prevalent in the East Asian population (38.4%) and least prevalent in North Americans (8.0%). No significant differences in AN prevalence were found when comparing different imaging modalities (X-ray and cadaver dissection).

Conclusion

Accessory navicular is a common finding in imaging studies. Its prevalence depends on the population covered by the study but is not affected by the patient’s gender or the imaging modality utilized for AN assessment.
Infant Botulism: In Search of Clostridium botulinum Spores
Authors: Harris Richard A. , Dabritz Haydee A.
Published: August 2024

Infant botulism is now the most common form of human botulism in Canada and the United States. Infant botulism is a severe neuroparalytic disease caused by ingestion of the spore-forming neurotoxic clostridia, including Clostridium botulinum that colonize the large intestine and subsequently produce botulinum neurotoxin in situ. It has been over a century since the first surveys documenting the ubiquitous prevalence of C. botulinum in soils around the world. Since then, honey has been identified as the only well-known risk factor for infant botulism despite a multitude of international environmental surveys isolating C. botulinum spores from ground soil, aquatic sediments, and commonly available infant foods. Associations of infant botulism cases with confirmed sources of C. botulinum exposure have primarily implicated outdoor soil and indoor dust, as well as commonly ingested foods including honey, dry cereals, and even powdered infant formula. Yet the origin of infection remains unknown for most infant botulism cases. This review summarizes the various surveys from around the world for C. botulinum in environmental soils and sediments, honey, and other infant foods, as well as laboratory-confirmed associations with documented infant botulism cases. Additional factors are also discussed, including the composition of infant gut microbiota and the practice of breastfeeding. We make several recommendations to better identify sources of exposure to C. botulinum spores that could lead to effective preventive measures and help reduce the incidence of this rare but life-threatening disease.
Image-based modeling of coupled electro-chemo-mechanical behavior of Li-ion battery cathode using an interface-modified reproducing kernel particle method
Authors: Susuki Kristen , Allen Jeffery , Chen Jiun-Shyan
Published: August 2024

An interface-modified reproducing kernel particle method (IM-RKPM) is introduced in this work to allow for a direct model construction from image pixels of heterogeneous polycrystalline Li-ion battery microstructures. The interface-modified reproducing kernel (IM-RK) approximation is constructed through scaling of a kernel function by a regularized distance function in conjunction with strategic placement of interface node locations. This leads to RK shape functions with either weak or strong discontinuities across material interfaces, suitable for modeling various interface mechanics. With the placement of a triple junction node and distance-based scaling of kernel functions, the resulting IM-RK shape function also possesses proper discontinuities at the triple junctions. This IM-RK approximation effectively remedies the well-known Gibb’s oscillation in the smooth approximation of discontinuities. Different from the conventional meshfree approaches for interface discontinuities, this IM-RK approach is done without additional degrees of freedom associated with the enrichment functions, and it is formulated with the standard procedures in the RK shape function construction. This work focuses on identifying the accuracy and convergence properties of IM-RKPM for modeling the coupled electro-chemo-mechanical system. A linear patch test is formulated and numerically tested for the electro-chemo-mechanical coupled problem with a Butler–Volmer boundary condition representing the physical conditions in Li-ion battery microstructures. This is followed by verification of the optimal rates of convergence of IM-RKPM for solving the coupled problem with higher order solutions. The image-based modeling of Li-ion battery microstructures in the numerical examples demonstrates the applicability of the proposed method to realistic Li-ion battery materials modeling.
Proposal and application of a convective wind gustiness parameterization based on convective available potential energy
Authors: Jing Xueyi , Wang Lanning , Wu Qizhong , Cheng Huaqiong
Published: August 2024

Wind gustiness is an effective approach for addressing mesoscale enhancement in estimating air-sea interface fluxes. In this study, an improved convective gustiness parameterization scheme based on convective available potential energy (CAPE) is formulated utilizing reanalysis data and wind observations from moored buoys. This new parameterization was examined by implementing it in the air-sea flux transfer scheme within the National Center for Atmospheric Research Community Earth System Model (NCAR CESM) version 2.1.3 and contrasting it with a previously proposed scheme based on convective precipitation rate. The results indicate a significant reduction in negative biases of surface winds and latent heat fluxes over the tropical Indian Ocean and western Pacific during summer with the implementation of either gustiness scheme, resulting in an average increase in latent heat flux of approximately 9 W·m− 2. Specifically, the CAPE-based scheme shows a more favorable impact in the Indo-Pacific Warm Pool region, whereas the precipitation-based scheme exhibits inferior performance in the western Pacific Intertropical Convergence Zone. Further analysis reveals that the inclusion of the gustiness scheme distinctly alters surface evaporation and latent heat flux, influencing vertical motion in the area with active convective activity and effectively improving precipitation simulations by up to 18%. Moreover, the CAPE-based scheme reduces the bias in summer precipitation simulations by approximately 5% more than the precipitation-based scheme.
Medical treatment of miscarriage using misoprostol—a retrospective study
Authors: Meister Laura , Künnemann Ines , Fettke Franziska , Lux Anke , Ignatov Atanas
Published: August 2024

Purpose

The treatment of early miscarriage with medication is effective and low in side effects. Nevertheless, no uniform dosage regimen has yet been established, nor has it been possible to determine whether previous pregnancies and births with their respective modes of delivery play a role in the effectiveness of Misoprostol. This study aimed to find predictive parameters for successful treatment with Misoprostol in early miscarriage.

Methods

In a retrospective study at the Otto von Guericke University Women’s Hospital, records of patients with early miscarriage and medical treatment using Misoprostol from 2018 to 2021 were reviewed for this purpose. The need for a curettage subsequent to treatment was scored as a parameter of failure. The data were analyzed using Statistical Package for the Social Science Version 28.0. The significance level was set to 0.050.

Results

We found that successful therapy with misoprostol was seen in 86% (n = 114). 14% (n = 20) of the patients had curettage after taking Misoprostol as advised. Out of 134 women, 16% (n = 21) reported mild side effects, with nausea as the leading one (9.2% (n = 12)). Significance was found comparing the measurement of double endometrial stripe thickness after the second cycle of Misoprostol in women with and without curettage after medical treatment (exact value two-sided 0.035 at p < 0.05). A cutoff value at 8.8 mm was calculated using ROC Analysis.

Conclusions

Our results indicate that the treatment of early miscarriage in the first trimester with Misoprostol is effective and has few side effects. The measurement of the endometrial stripe thickness after the second cycle of Misoprostol via transvaginal ultrasound could present a predictive marker during therapy.
Personal inhalable paper dust exposure and potential determinants among paper industry workers in Ethiopia
Authors: Tafese Ararso , Kumie Abera , Abegaz Teferi , Abaya Samson Wakuma , Moen Bente E. , Deressa Wakgari , Bråtveit Magne
Published: August 2024

Purpose

Excessive paper dust during paper production may harm the workers’ respiratory health. We wanted to assess the inhalable paper dust levels and its determinants among paper industry workers.

Methods

A study was conducted in Ethiopia to assess the level of personal inhalable paper dust exposure among four paper mills. A total of 150 samples were collected using the IOM sampler attached to Side Kick Casella pumps at a flow rate of 2 L/min. The samples were analyzed in Nemko Norlab, Norway. Linear mixed-effect models were applied to identify determinants of inhalable paper dust.

Results

The geometric mean of personal inhalable paper dust was 3.3 mg/m3 with 80% of the measurements exceeding the Swedish occupational exposure limit (OEL) of 2 mg/m3. The linear mixed-effects model showed that the level of dust was 28% higher when using high-speed than when using low-speed rewinding machines, while paper mills with an average of more than four machines per job group had 22% higher exposure than paper mills with a lower number of machines. Furthermore, working in packing and preparation was associated with higher dust exposure than in other areas.

Conclusions

The dust exposure levels were above the Swedish OEL for 80% of the samples. This indicates that preventive measures should be established in the industry. The exposure model identified high-speed rewinding machines, a high number of machines, and work in preparation and packing as associated with high levels of paper dust exposure.
Cardiorespiratory and muscle oxygenation responses to voluntary hypoventilation at low lung volume in upper body repeated sprints
Authors: Rosa Cristóvão H. , Monteiro Cristina P. , Barata Cláudia , Espada Mário C. , Valamatos Maria João , Bento André , Minhalma Ricardo J. , Reis Joana Filipa
Published: August 2024

Purpose

To investigate the impact of voluntary hypoventilation at low lung volumes (VHL) during upper body repeated sprints (RS) on performance, metabolic markers and muscle oxygenation in Brazilian Jiu-Jitsu (BJJ) athletes.

Methods

Eighteen male well-trained athletes performed two randomized RS sessions, one with normal breathing (RSN) and another with VHL (RS-VHL), on an arm cycle ergometer, consisting of two sets of eight all-out 6-s sprints performed every 30 s. Peak (PPO), mean power output (MPO), and RS percentage decrement score were calculated. Arterial oxygen saturation (SpO2), heart rate (HR), gas exchange, and muscle oxygenation of the long head of the triceps brachii were continuously recorded. Blood lactate concentration ([La]) was measured at the end of each set. Bench press throw peak power (BPPP) was recorded before and after the RS protocol.

Results

Although SpO2 was not different between conditions, PPO and MPO were significantly lower in RS-VHL. $${\dot{\text{V}}}$$

V
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E, HR, [La], and RER were lower in RS-VHL, and VO2 was higher in RS-VLH than in RSN. Muscle oxygenation was not different between conditions nor was its pattern of change across the RS protocol influenced by condition. [La] was lower in RS-VHL than in RSN after both sets.

Conclusion

Performance was significantly lower in RS-VHL, even though SPO2 was not consistent with hypoxemia. However, the fatigue index was not significantly affected by VHL, nor was the neuromuscular upper body power after the RS-VHL protocol. Additionally, [La] was lower, and oxygen consumption was higher in RS-VHL, suggesting a higher aerobic contribution in this condition.
Ten years of detecting Neoehrlichia mikurensis infections in Sweden: demographic, clinical and inflammatory parameters
Authors: Wennerås Christine , Wass Linda , Bergström Beatrice , Grankvist Anna , Lingblom Christine
Published: August 2024

Purpose

To increase knowledge about the varied clinical manifestations of human infection with the emerging tick-borne pathogen Neoehrlichia mikurensis.

Methods

All patients diagnosed in Sweden with N. mikurensis infection during a 10-year period (2013–2023) were investigated regarding their demographic factors, risk factors, comorbidities, clinical signs and symptoms, and laboratory results. Multivariate models were generated using “Orthogonal Projections to Latent Structures-Discriminant Analysis” to identify clinical and immune parameters associated with N. mikurensis infection.

Results

During the 10-year period, 134 patients were diagnosed with N. mikurensis infection, 102 of whom were included in this study. Most of the patients (79%) were immunosuppressed. The main comorbidities were malignant B-cell lymphomas, multiple sclerosis, and rheumatoid arthritis. Rituximab therapy (59%) and splenectomy (14%) featured prominently. All patients resided in the southern tick-endemic part of Sweden, yet one-third of them were diagnosed in wintertime when ticks are inactive. Two asymptomatically infected blood donors were identified but transfusion-transmitted infection was not confirmed. Increased levels of C-reactive protein, orosomucoid, and total IgM in serum were associated with neoehrlichiosis. Previously unreported symptoms such as ankle edema, neck pain, numbness, and sudden deafness were detected in some patients. One case of aplastic anemia partially improved after eradication of the infection.

Conclusions

Neoehrlichiosis is a multi-faceted emerging infectious disease.
Feasibility and outcomes of robotic sphincter-preserving surgery for rectal cancer after neoadjuvant treatment in patients with preexisting colostomy
Authors: Nozawa H. , Sakamoto A. , Murono K. , Sasaki K. , Emoto S. , Ishihara S.
Published: August 2024

Background

Diverting colostomy followed by neoadjuvant treatment is a treatment of choice for obstructive rectal cancer. Such patients may be treated via a robotic approach with several advantages over conventional laparoscopic surgery. Conversely, the existing stoma may interfere with the optimal trocar position and thus affect the quality of robotic surgery. Moreover, the console surgeon does not face the patient, which may endanger the stoma.

Methods

Patients with rectal cancer who underwent sphincter-preserving surgery were retrospectively investigated using a robotic platform after neoadjuvant treatment at our hospital. Based on pretreatment stoma creation, patients were divided into the NS (those without a stoma) and S groups (patients with a stoma). Baseline characteristics, types of neoadjuvant treatment, short-term surgical outcomes, postoperative anorectal manometric data, and survival were compared between the groups.

Results

The NS and S groups comprised 65 and 9 patients, respectively. Conversion to laparotomy was required in three patients in the NS group. The S group required a longer console time than the NS group (median: 367 vs. 253 min, respectively, p = 0.038); however, no difference was observed in the total operative time (p = 0.15) and blood loss (p = 0.70). Postoperative complication rates, anorectal function, and oncological outcomes were similar between the groups.

Conclusions

Although console time was longer in patients with a stoma, robotic surgery could be performed safely like in those without a stoma after neoadjuvant treatment.
The Current State of Tinnitus Diagnosis and Treatment: a Multidisciplinary Expert Perspective
Authors: Kleinjung Tobias , Peter Nicole , Schecklmann Martin , Langguth Berthold
Published: August 2024

Tinnitus, the perception of sound without an external source, affects 15% of the population, with 2.4% experiencing significant distress. In this review, we summarize the current state of knowledge about tinnitus management with a particular focus on the translation into clinical practice. In the first section, we analyze shortcomings, knowledge gaps, and challenges in the field of tinnitus research. Then, we highlight the relevance of the diagnostic process to account for tinnitus heterogeneity and to identify all relevant aspects of the tinnitus in an individual patient, such as etiological aspects, pathophysiological mechanisms, factors that contribute most to suffering, and comorbidities. In the next section, we review available treatment options, including counselling, cognitive-behavioral therapy (CBT), hearing aids and cochlear implants for patients with a relevant hearing loss, sound generators, novel auditory stimulation approaches, tinnitus retraining therapy (TRT), pharmacological treatment, neurofeedback, brain stimulation, bimodal stimulation, Internet- and app-based digital approaches, and alternative treatment approaches. The evidence for the effectiveness of the various treatment interventions varies considerably. We also discuss differences in current respective guideline recommendations and close with a discussion of how current pathophysiological knowledge, latest scientific evidence, and patient perspectives can be translated in patient-centered care.
The value of perforator flap reconstruction in painful soft tissue calcifications
Authors: Kouba Loraine , Fabi Adriano , Glatz Kathrin , Thoma Anna , Lariu Ana , Burger Maximilian , Schweizer Thierry , Schaefer Dirk J. , Kappos Elisabeth A.
Published: August 2024

Soft tissue calcifications frequently cause debilitating pain and functional impairments, considerably affecting patients’ quality of life. As they are rare entities, evidence remains sparse, especially regarding treatment effectiveness and recurrence rates. While both pharmacological and surgical treatments may alleviate symptoms, complete resection is currently believed to prevent long-term recurrence of deposits. To improve understanding and raise awareness for soft tissue calcifications, the goal of this study was to review the current state of treatment and to compare benefits and possibilities of flap reconstruction versus simple excision in improving quality of life. Furthermore, we include a successful case report of complete resolution of symptoms following quadruple perforator flap reconstruction. By systematic literature review, studies published in MEDLINE between 1980 and 2024 reporting on surgical treatment and outcome of soft tissue calcifications were included, in addition to a detailed description of our case report. A total of 53 studies reporting on 197 patients with soft tissue calcifications were included. Simple surgical excision was the most commonly (85.9%) employed procedure, demonstrating a substantial recurrence rate of 13.3%. In contrast, no patients who underwent radical excision experienced recurrence. Dermal matrix grafts and flap reconstruction were successfully used in patients requiring substantial tissue coverage, highlighting their value in complex defect reconstruction following radical excision. The combination of complete surgical resection and flap reconstruction reduces recurrence rates and improves postoperative outcomes and quality of life of these patients, supporting early radical surgical intervention as the gold standard treatment for soft tissue calcifications.
Are physical and mechanical properties of 3D resins dependent on the manufacturing method?
Authors: Rizzante Fabio , Hales Hannah , Teich Sorin , Furuse Adilson Yoshio , Mendonça Gustavo , Brennes Christian
Published: August 2024

This research analyzed the effect of the manufacturing method on the flexural strength and color stability of 3D-printed resins used for producing indirect restorations. For this, two dental restorative biocompatible resin materials, OnX (OnX, SprintRay) and CB (Crown and Bridge, Dentca), were divided into 2 groups according with manufacturing method (printed with a Pro95 3D printer – SprintRay; and not printed, with samples obtained with the fluid resin being poured on PVS molds for further light activation in the post-curing process), and subdivided into 2 groups according to the post-curing method: VG (Valo Grand, Ultradent Products) for 120 s and PC (Procure 2, SprintRay). Bar-shaped samples were used to evaluate the flexural strength 24 h after storage in distilled water at 37 °C using a universal testing machine. Disk-shaped samples were used to evaluate the color stability with a spectrophotometer at baseline, after 1–7 days in dark dry storage at 37 °C, and after 1 day of artificial aging in water at 60 °C. Data were evaluated using 3-way ANOVA (flexural strength) and 4-way repeated measures ANOVA (color stability), followed by the Tukey’s HSD test (α = .05). Flexural strength showed significant results for resin (p < .001), while manufacturing and post-curing methods were not significant (p > .05). The interaction effects between resin and manufacturing method (p = .978), and between resin, manufacturing method and post-curing method (p = .659) were not significant. In general, OnX showed higher flexural strength values than CB, regardless of manufacturing method or post-curing protocol. Color stability results showed significant results for resin (p < .001), time (p < .001), resin and time (p = .029), and resin and curing method (p < .001), but no differences considering resin and manufacturing mode (p = .87), or resin, manufacturing method and curing method (p = .35). In general, OnX showed a higher color change than CB, longer storage times resulted in increased color change for both materials, and CB cured with VG showed lower color alteration than CB cured with PC2. The manufacturing method (3D printed or not 3D printed) does not seem to influence the flexural strength and color stability of 3D printed resins. This may indicate that, at least from a physical–mechanical perspective, the final properties of the material are mainly dependent on the post-curing process.
Canopy openness, proportion of deciduous trees and topsoil C/N ratio drive the yield, but their effect on the polyphenol content of medicinal plants is species-specific
Authors: Jaroszewicz Bogdan , Stojek Katarzyna , Bruelheide Helge , Ponette Quentin , Scherer-Lorenzen Michael , Verheyen Kris , Kiss Anna K.
Published: August 2024

Forests and woodlands are the major source of wild medicinal plants worldwide. In our study, we aimed to identify the factors influencing the yield and polyphenol content of Aegopodium podagraria L., Galium aparine L., Rubus fruticosus L., Rubus idaeus L., Stachys sylvatica L. and Urtica dioica L., the common and abundant medicinal plant species in the study areas. We showed that European temperate forests are potentially an important source of the medicinal resources. Light availability, controlled by canopy cover, proportion of deciduous trees and stand basal area were the most important factors positively influencing both abundance and quality of medicinal plants. The C/N ratio and pH of the topsoil were the most important factors positively influencing the content of phenolic compounds. The phenolic content was highly species-specific and varied according to local environmental conditions. A high proportion of deciduous species and a high canopy openness increased the yield and quality of medicinal plants by ensuring high light availability. Plants with high total polyphenol content should also be sought on biologically active (non-acidic) soils with a high C/N ratio. Our results can be used to guide forest management in areas where harvesting of understory medicinal plants is an important provisioning ecosystem service. In many cases a forest management scenario friendly to medicinal plants may require only a minor changes in forest management intensity, as cultivation or enhanced growth of MD plants can take place in intensively thinned forests and cleared forest patches, without competing with timber production.
Automatic Detection of Fatigued Gait Patterns in Older Adults: An Intelligent Portable Device Integrating Force and Inertial Measurements with Machine Learning
Authors: Zhang Guoxin , Hong Tommy Tung-Ho , Li Li , Zhang Ming
Published: August 2024

Purpose

This study aimed to assess the feasibility of early detection of fatigued gait patterns for older adults through the development of a smart portable device.

Methods

The smart device incorporated seven force sensors and a single inertial measurement unit (IMU) to measure regional plantar forces and foot kinematics. Data were collected from 18 older adults walking briskly on a treadmill for 60 min. The optimal feature set for each recognition model was determined using forward sequential feature selection in a wrapper fashion through fivefold cross-validation. The recognition model was selected from four machine learning models through leave-one-subject-out cross-validation.

Results

Five selected characteristics that best represented the state of fatigue included impulse at the medial and lateral arches (increased, p = 0.002 and p < 0.001), contact angle and rotation range of angle in the sagittal plane (increased, p < 0.001), and the variability of the resultant swing angular acceleration (decreased, p < 0.001). The detection accuracy based on the dual signal source of IMU and plantar force was 99%, higher than the 95% accuracy based on the single source. The intelligent portable device demonstrated excellent generalization (ranging from 93 to 100%), real-time performance (2.79 ms), and portability (32 g).

Conclusion

The proposed smart device can detect fatigue patterns with high precision and in real time. Significance: The application of this device possesses the potential to reduce the injury risk for older adults related to fatigue during gait.
Factors Associated with Usage of Oral-PrEP among Female Sex Workers in Nairobi, Kenya, Assessed by Self-Report and a Point-of-Care Urine Tenofovir Immunoassay
Authors: Shah Pooja , Spinelli Matthew , Irungu Erastus , Kabuti Rhoda , Ngurukiri Pauline , Babu Hellen , Kungu Mary , Champions The Maisha Fiti Study , Nyabuto Chrispo , Mahero Anne , Devries Karen , Kyegombe Nambusi , Medley Graham F. , Gafos Mitzy , Seeley Janet , Weiss Helen A. , Kaul Rupert , Gandhi Monica , Beattie Tara S. , Kimani Joshua
Published: August 2024

Pre-exposure prophylaxis (PrEP) is highly effective at reducing HIV acquisition. We aimed to estimate usage of oral-PrEP, and factors associated with adherence among female sex workers (FSWs) in Nairobi, Kenya, using a novel point-of-care urine tenofovir lateral flow assay (LFA). The Maisha Fiti study randomly selected FSWs from Sex Worker Outreach Program clinics in Nairobi. Data were collected from 1003 FSWs from June-October 2019, including surveys on self-reported oral-PrEP adherence. Adherence was also measured using the LFA for HIV-negative FSWs currently taking oral-PrEP. Informed by a social-ecological theoretical framework, we used hierarchical multivariable logistic regression models to estimate associations between individual, interpersonal/community, and structural/institutional-level factors and either self-reported or LFA-assessed adherence. Overall, 746 HIV-negative FSWs aged 18–40 participated in the study, of whom 180 (24.1%) self-reported currently taking oral-PrEP. Of these, 56 (31.1%) were adherent to oral-PrEP as measured by LFA. In the multivariable analyses, associations with currently taking oral-PrEP included having completed secondary education, high alcohol/substance use, feeling empowered to use PrEP, current intimate partner, no recent intimate partner violence, having support from sex worker organisations, experiencing sex work-related stigma, and seeking healthcare services despite stigma. Associations with oral-PrEP LFA-measured adherence measured included having only primary education, experience of childhood emotional violence, belonging to a higher wealth tertile, and being nulliparous. Oral-PrEP adherence, measured by self-report or objectively, is low among FSWs in Nairobi. Programs to improve oral-PrEP usage among FSWs should work to mitigate social and structural barriers and involve collaboration between FSWs, healthcare providers and policymakers.
Should one (be allowed to) replace the Cipollini’s?
Authors: Ausloos Marcel
Published: August 2024

One examines and discusses proposals on whether riders could be replaced in a team during multi-stage races, and how much a team final time at the end of the race would change (be "adjusted") if only the riders having completed the race are taken into account for ranking teams. A few results of the two main multi-stage races, the men Tour de France and the Giro d’Italia, are used as case studies. The impact of disqualification later on, due to doping, much after the end of such a race, is also examined in the case of two Tour de France. The statistical discussion is based on the Kendall-$$\tau $$τcoefficients for comparing team ranks at the end of these multi-stages races cases. One observes that there are significant differences in the results of the discussed measures. It is shown that there is much variety in results significance, whence demonstrating many interests of the "adjusted indicators". Moreover, it is argued that the "adjusted" rank indicator would promote more competitive and more attractive daily stages and lead to more valuable race management.
Screening persistent organic pollutants for effects on testosterone and estrogen synthesis at human-relevant concentrations using H295R cells in 96-well plates
Authors: Strand Denise , Nylander Erik , Höglund Andrey , Lundgren Bo , Martin Jonathan W. , Karlsson Oskar
Published: August 2024

Many persistent organic pollutants (POPs) are suspected endocrine disruptors and it is important to investigate their effects at low concentrations relevant to human exposure. Here, the OECD test guideline #456 steroidogenesis assay was downscaled to a 96-well microplate format to screen 24 POPs for their effects on viability, and testosterone and estradiol synthesis using the human adrenocortical cell line H295R. The compounds (six polyfluoroalkyl substances, five organochlorine pesticides, ten polychlorinated biphenyls and three polybrominated diphenyl ethers) were tested at human-relevant levels (1 nM to 10 µM). Increased estradiol synthesis, above the OECD guideline threshold of 1.5-fold solvent control, was shown after exposure to 10 µM PCB-156 (153%) and PCB-180 (196%). Interestingly, the base hormone synthesis varied depending on the cell batch. An alternative data analysis using a linear mixed-effects model that include multiple independent experiments and considers batch-dependent variation was therefore applied. This approach revealed small but statistically significant effects on estradiol or testosterone synthesis for 17 compounds. Increased testosterone levels were demonstrated even at 1 nM for PCB-74 (18%), PCB-99 (29%), PCB-118 (16%), PCB-138 (19%), PCB-180 (22%), and PBDE-153 (21%). The MTT assay revealed significant effects on cell viability after exposure to 1 nM of perfluoroundecanoic acid (12%), 3 nM PBDE-153 (9%), and 10 µM of PCB-156 (6%). This shows that some POPs can interfere with endocrine signaling at concentrations found in human blood, highlighting the need for further investigation into the toxicological mechanisms of POPs and their mixtures at low concentrations relevant to human exposure.

Graphical Abstract
Government participation in virtual negotiations: evidence from IPCC approval sessions
Authors: Bayer Patrick , Crippa Lorenzo , Hughes Hannah , Hermansen Erlend
Published: August 2024

The Covid-19 pandemic challenged global governance in unprecedented ways by requiring intergovernmental meetings to be held online. For the Intergovernmental Panel on Climate Change (IPCC), this meant that the intergovernmental approval of the key findings of the Sixth Assessment Report (AR6) had to be conducted virtually. In this paper, we assess how the move away from face-to-face meetings affected country participation in IPCC approval sessions. Our findings demonstrate that virtual meetings increased the size of member governments’ delegations, but this did not necessarily translate into a greater number of interventions during the approval of the Summary for Policymakers (SPM) as time zone differences reduced engagement levels significantly—particularly for countries from the Pacific, East Asian, and Latin American regions whose delegations often found themselves in IPCC meetings late at night and early in the morning. These results offer initial, empirically robust evidence about what online meetings can and cannot achieve for promoting more inclusive global governance at a time when the IPCC and other organizations reflect on the future use of virtual and hybrid meeting formats.
Urbanisation has impacted the population genetic structure of the Eurasian red squirrel in Japan within a short period of 30 years
Authors: Takahata Yu , Uchida Kenta , Kutsukake Nobuyuki , Shimamoto Tatsuki , Asari Yushin , Terai Yohey
Published: August 2024

Human activities often induce maladaptive effects in urban wildlife, such as increased inbreeding and decreased gene flow, consequently leading to a loss of biodiversity. Although maladaptive effects are commonly reported in urban wildlife, some wildlife species are not affected. Understanding the conditions and factors that affect the genetic structure of wildlife is crucial for its conservation in urban environments. Eurasian red squirrels Sciurus vulgaris in Obihiro City, Hokkaido, Japan, have settled in large residential lots with a high risk of vehicle collisions for approximately 30 years, raising the possibility that the urban population would be genetically isolated from nearby rural populations and may experience a rapid decrease in genetic diversity. In this study, we analysed the population structure, genetic distance, and nucleotide diversity of squirrels in the Obihiro area using genome-wide single nucleotide polymorphisms (SNPs). Principal component analysis and ADMIXTURE showed that urban and rural squirrel populations formed distinct clusters with intermediate urban and rural boundary populations. Genetic distances between the urban populations were greater than that of other pairs. Nucleotide diversities were lower in urban populations than in other populations, suggesting limited gene flow between urban and other populations, possibly due to urbanisation. The change in the genetic characteristics of urban squirrel populations during this short period demonstrates the strong impact of urbanisation. Our findings suggest that a genome-wide approach to the genetic structure of wildlife contributes to its conservation in urban environments.
The Effects of Growing Groups and Scarcity on the Use of a Common Pool Resource – a Lab-in-the-Field Experiment with Lake Victoria Fishers
Authors: Dannenberg Astrid , Klatt Charlotte , Pico Pia
Published: August 2024

Using a lab-in-the-field experiment with Ugandan fishers, we study if and how the use of a common pool resource changes when the resource is either scarce or abundant and when the number of users increases over time. Both resource scarcity and a growing group require users to be more constrained, that is, more cooperative, in order to maintain the resource. However, the results show that fishers do not curtail their harvesting behavior under increased pressure, leading to rapid overexploitation when scarce resources are used by a growing group. This implies a particular need for sustainable management when scarce resources are exposed to in-migration.
Distance: A 2024 re-examination
Authors: Kellerman Aharon
Published: August 2024

Distance, a concept that continues to be relevant and rather complex, is far from being obsolete despite its diminishing role in the age of virtual space. It represents spatial separation, a notion that was first quantified in Egypt some 5,000 years ago. The evolution of distance can be traced through five distinct phases: natural (absolute and relative distances), relational (time and cost distances, distance decay, friction of distance, and distanciation), technological (extensibility, accessibility, and speed), virtual (proximity, digital, cognitive, and metaphorical distances), and optional (optional and estimated distances). These historical phases have built upon each other, leading to our contemporary experience of all distance classes depending on specific separation cases. Modern humans may overcome any distance on earth, one way or another, unless prevented from doing so by political national sovereignties.
Riemannian Newton Methods for Energy Minimization Problems of Kohn–Sham Type
Authors: Altmann R. , Peterseim D. , Stykel T.
Published: August 2024

This paper is devoted to the numerical solution of constrained energy minimization problems arising in computational physics and chemistry such as the Gross–Pitaevskii and Kohn–Sham models. In particular, we introduce Riemannian Newton methods on the infinite-dimensional Stiefel and Grassmann manifolds. We study the geometry of these two manifolds, its impact on the Newton algorithms, and present expressions of the Riemannian Hessians in the infinite-dimensional setting, which are suitable for variational spatial discretizations. A series of numerical experiments illustrates the performance of the methods and demonstrates their supremacy compared to other well-established schemes such as the self-consistent field iteration and gradient descent schemes.