bluuebunny/crossref_metadata_2025 huggingface dataset:
Parallel reliability-guided algorithm for digital image correlation
Authors: Lin Zhe , Cai Tian , Wang Yanfeng
Published: December 2019

Digital image correlation is a non-contact optical method for measuring the displacement and strain on the surface of a material. The existing reliability-guided digital image correlation (RG-DIC) method is stable and reliable for a single image but it still needs a large calculational
resource for a sequence of images. Due to the decorrelation effect, the reference image must be replaced several times to correct the measurement results for an image sequence involving a large deformation or a discontinuous deformation. Since the process must be executed sequentially, image
by image, the total time required is often unacceptably large when the image sequence is long. The challenge is to find a way of improving the speed while retaining calculational reliability and measurement accuracy, which are important for the practical application of DIC. To address this
problem, an improved method is proposed in this paper. The parallel bottleneck caused by the decorrelation effect is solved through improving the parallelism to increase the processing speed. This approach can be used to calculate the strain field of the surface of the material in cases of
discontinuous deformation, such as in the area near to a crack. Compared with existing methods, this method not only retains the calculational reliability but also greatly improves calculation speed, especially on current multi-core computing platforms.
Identificação de Resíduos em uma Unidade de Alimentação e Nutrição
Authors: Mariosa Silmara Thais , Alves Márcia Keller
Published: December 2019

A análise quantitativa da geração de resíduos sólidos no setor de refeições coletivas pode ser usada como indicativo de desperdício em uma Unidade de Alimentação e Nutrição (UAN), prevenindo perdas econômicas e reduzindo o impacto ambiental causado pelos resíduos. O estudo teve como objetivo quantificar a geração de resíduos sólidos em uma UAN inserida em uma empresa do ramo metalúrgico. Foram avaliados os resíduos sólidos durante cinco dias alternados, sendo um dia em cada semana. Para servir 1.407 refeições foram gerados 134.925 g de resíduos sólidos alimentares, resultando em um per capita de 95,89 g/pessoa e 27.114 g de resíduos sólidos não alimentares, per capita de 19,27 g/pessoa. Do total de resíduos, 32,17% foram gerados no pré-preparo; 21,21% foram gerados no resto ingestão; 19,21% foram de sobra suja; 18,80% foram de resíduos não alimentares e 7,90% referentes à sobra limpa. Conclui-se que a UAN apresentou uma quantidade de resíduos sólidos per capita inferior aos valores reportados pela literatura, porém o controle de desperdício deve ser aplicado, constantemente, para dar continuidade na redução do desperdício alimentar e na geração de resíduos. Palavras-chave: Resíduos Sólidos. Nutrição. Desperdício de Alimentos. AbstractThe quantitative analysis of waste generation in the collective meal sector can be used as an indicator of waste in a Food and Nutrition Unit (FNU), preventing economic losses and reducing the environmental impact caused by the waste. This study aimed to quantify solid waste generation in a FNU inserted in a metallurgical company. The residues were evaluated during five alternate days, being one day in each week. In order to  serve 1,407 meals, 134,925 g of solid food waste was generated, resulting in a per capita of 95.89 g/person and 27,114 g of non-food solids per capita of 19.27 g/person. Of the total waste, 32.17% was generated in the pre-preparation; 21.21% was generated in the rest ingestion; 19.21% was dirty leftover food; 18.80% was disposable waste and 7.90% was clean waste. It is concluded that FNU presented a quantity of solid waste per capita lower than the values reported in the literature, but the waste control must be applied constantly to continue the reduction of food waste and waste generation. Keywords: Solid Residues. Nutrition Unit. Food Waste.
Análise da Tendência Climática nas Séries Temporais de Temperatura de Rondonópolis - MT
Authors: Batista Danielle Da Silva , Novais Jonathan Willian Zangeski
Published: December 2019

Com expansão territorial, as cidades tendem a sofrer com as alterações climáticas, estudar essas alterações se apresentam como de extrema necessidade, pois auxiliarão na mitigação desses efeitos para a sociedade. O objetivo deste trabalho foi analisar séries temporais de temperatura média da cidade de Rondonópolis-MT no período de 2004 a 2016, os dados foram coletados pelo Instituto Nacional Meteorológico (INMET), obtidos por meio de seu Banco de Dados Meteorológicos para Ensino e Pesquisa (BDMEP), para essa análise foi utilizado o teste não paramétrico de Mann Kendall. Os resultados apresentaram tendência positiva, porém não significativa, ou seja, houve aumento na temperatura média durante o período estudado. Os meses de janeiro e fevereiro apresentaram o valor de ZMK -0,011 e -0,055 respectivamente, dessa forma o valor de ZMK<0 consequentemente decrescente, isso pode ter relação com o período chuvoso na região. Palavra-chave: Mudança Climática. Microclima. Urbanização. AbstractWith territorial expansion cities tend to suffer from climate change, studying these changes are extremely necessary, as it will help in mitigating these effects to the society. The objective of this work was to analyze the temporal series of the mean temperature of the city of Rondonópolis-MT from 2004 to 2016, the data were collected by the National Meteorological Institute (INMET), obtained through its Meteorological Database for Teaching and Research BDMEP), the non-parametric Mann Kendall test was used for this analysis. The results showed a positive tendency, but not significant, that is, there was an increase in the average temperature during the studied period. The months of January and February presented the value of ZMK -0.011 and -0.055 respectively, thus the value of ZMK <0 consequently decreasing, this may be related to the rainy season in the region. Keywords: Climate Change. Microclimate. Urbanization.
Rotulagem de Ovos e Mel: Avaliação de Conformidades à Legislação Vigente
Authors: Bazanella Pricila Carla , Alves Márcia Keller
Published: December 2019

A rotulagem de gêneros alimentícios constitui uma importante ferramenta de comunicação entre o setor alimentar e os consumidores, permitindo não somente fornecer informações dos produtos, como também ajudar na garantia da sua segurança. Desse modo, o objetivo deste estudo foi descrever o levantamento das não conformidades da rotulagem de ovos e de mel de supermercados de Caxias do Sul, Rio Grande do Sul. Tratou-se de um estudo descritivo, no qual se avaliaram 24 rótulos selecionados aleatoriamente de mel e de ovos, considerando os seguintes critérios: identificação do produto, segurança sanitária, qualidade técnica do rótulo, qualidade de informação e informação sobre o atendimento ao consumidor. Os resultados foram analisados de forma descritiva, estimando-se frequências simples das variáveis do estudo por tipo de alimento. Verificou-se que todos os rótulos atendiam a legislação em vigor ao que diz respeito à identificação do produto e à qualidade técnica do rótulo. Nos demais critérios foram encontrados não conformidades, sendo as principais as que dizem respeito ao armazenamento e conservação e informações sobre o atendimento ao consumidor. Os autores consideram que os rótulos dos produtos analisados, de modo geral, apresentaram qualidade sanitária satisfatória, pois os aspectos que apresentaram maior percentual de inconformidades não devem ser considerados de risco para a saúde do consumidor. Palavras-chave: Alimentos Industrializados. Alimentos de Origem Animal. Legislação sobre Alimentos. AbstractFood labeling is an important communication tool between the food industry and consumers, allowing not only to provide product information but also to assist in ensuring its safety. Thus, the objective of this study was to describe the nonconformities of eggs and honey labeling from supermarkets in Caxias do Sul, Rio Grande do Sul, Brazil. This was a descriptive study, in which 24 randomly honey and eggs selected labels were evaluated, considering the following criteria: product identification, health safety, the lable technical quality, quality of information and information on customer service. The results were analyzed in a descriptive way, estimating simple frequencies of the study variables by type of food. It was found that all labels complied with the legislation in force regarding the product identification and the label technical quality. In the other criteria, nonconformities were found, the main ones being those related to storage and conservation and information on consumer service.  The authors consider that the labels of the analyzed products, in general, presented satisfactory sanitary quality, since the aspects that presented greater percentage of nonconformities should not be considered of risk to the consumer health. Keywords: Food Labeling. Animal Origin Foods. Industrialized Foods.
ANÁLISE DAS VESTIMENTAS FÚNEBRES DE CRIANÇAS SEPULTADAS NA IGREJA NOSSA SENHORA DO PILAR NO ANO DE 1836, SALVADOR
Authors: Araújo Rebeca Oliveira , Bovkalovski Etiane Caloy
Published: December 2019

O presente artigo analisa os usos das mortalhas em crianças enterradas na Igreja Nossa Senhora do Pilar, no ano de 1836, em Salvador (BA). Sendo que o objetivo geral se propôs em analisar o motivo da escolha de determinadas mortalhas utilizadas por crianças, com mais especificidade diferenciar as roupas usadas por meninas e meninos enterradas na Igreja Nossa Senhora do Pilar; pesquisar sobre a relação entre o batismo e uso da mortalha nas crianças; investigar a devoção aos santos através do uso das mortalhas. A pesquisa se pauta na metodologia de análise de conteúdo, com abordagem quantitativa, proposta por Bardin. As fontes utilizadas foram registros de óbitos da Igreja Nossa Senhora do Pilar, no ano de 1836.
FORMAÇÃO HUMANA E EDUCAÇÃO: CONTRAPONTO À RACIONALIDADE E À INDÚSTRIA CULTURAL
Authors: Tavares Rosa Cleudes Maria
Published: December 2019

O presente artigo objetiva compreender a educação e a formação humana na sociedade contemporânea. Sociedade que apresenta índices altos de sofrimento. Entende-se, aqui, que tal sofrimento advém da racionalidade constitutiva e constituinte das relações que marcam a mesma, sejam relações objetivas ou subjetivas. E questiona-se qual a contribuição da educação para a superação deste contexto. Para tanto, o presente artigo ancora-se na teoria crítica frankfurtiana, especialmente em Adorno e Horkheimer, cujas reflexões teóricas e metodológicas propiciam a compreensão da tensão entre sujeito e objeto, particular e universal, indivíduo e sociedade. Para a discussão, são centrais as categorias trabalho, cultura e barbárie, formação humana e educação, racionalidade e sofrimento.
EDUCAÇÃO E FORMAÇÃO HUMANA
Authors: Scarel Estelamaris Brant
Published: December 2019

Apresentação do Dossiê.
PENSAR A FORMAÇÃO HUMANA
Authors: Neves Silva Rosemary Francisca
Published: December 2019

Editorial do Volume 29, n. 2 de 2019.
Introduction to NJWLS 2019-4
Authors: Buch Anders
Published: December 2019

This issue offers four new contributions to Nordic working life studies (...)
Industry 4.0: Whose Revolution? The Digitalization of Manufacturing Work Processes
Authors: Thun Sylvi , Kamsvåg Pål F. , Kløve Birgit , Seim Eva A. , Torvatn Hans Y.
Published: December 2019

The ongoing digitalization of manufacturing work processes resulting from Industry 4.0—defined as digitalization, automation, and data exchange in manufacturing—challenges how we see and define the role of operators and managers. Consequently, this study investigates the extent to which digital tools are used and available to managers and operators in manufacturing who are experiencing digitalization due to Industry 4.0 movements. A cross-sectional study of production managers and operators (n = 417) was conducted among 10 Norwegian manufacturing companies. Results from independent t-tests and Chi-square tests indicate that, compared with operators, production managers report higher satisfaction with different digitalization experiences, more extensive use of digital systems for registration and documentation, and greater availability of digital tools. Thus, digitalization and digital tools based on the Industry 4.0 concepts seem to have only reached the managerial level, and the revolution seems to be top down.
Programa Eureka en la resolución de problemas matemáticos en estudiantes de tercer grado de primaria
Authors: Sánchez Cosar Sonia Vilma
Published: December 2019

El objetivo de esta investigación fue determinar si existían cambios notables al aplicar el programa Eureka en la resolución de problemas matemáticos en estudiantes de tercer grado de la Institución Educativa José Gálvez Barrenechea, en Tarma (Perú), durante 2017. El programa Eureka es un conjunto de actividades planificadas con el método de Pólya para la resolución de problemas matemáticos, considerando cuatro etapas: comprender el problema, elaborar un plan, ejecutar el plan y realizar una visión retrospectiva. En el estudio se empleó el método científico, experimental y estadístico.

Los resultados permitieron apreciar que los promedios variaron significativamente en el pre y el postest. En el pretest de resolución de problemas matemáticos el grado “3-B” obtuvo un puntaje de 12.96, el “3-C” logró 12.43 y el “3-D” alcanzó 12.05; mientras que en el postest de resolución de problemas matemáticos el GC (“3-B”) logró 13.00, el GE1 (“3-C”) obtuvo 16.50 y el GE2 (“3-D”) consiguió 15.16. El programa Eureka influyó significativamente en la resolución de problemas, y se concluyó que hubo variaciones significativas en la resolución de problemas matemáticos, a través del Test anova con α = 0.05 de significancia, γ = 0.95 de confianza, además F calculada es mayor que F teórica (20.65 > 3.13), quedando demostrada la eficacia del programa Eureka.
Tumors and the danger model.
Authors: Kowalczyk Dariusz W
Published: December 2019

This article reviews the evidence for the danger model in the context of immune response to tumors and the insufficiency of the immune system to eliminate tumor growth. Despite their potential immunogenicity tumors do not induce significant immune responses which could destroy malignant cells. According to the danger model, the immune surveillance system fails to detect tumor antigens because transformed cells do not send any danger signals which could activate dendritic cells and initiate an immune response. Instead, tumor cells or antigen presenting cells turn off the responding T cells and induce tolerance. The studies reviewed herein based on model tumor antigens, recombinant viral vectors and detection of tumor specific T cells by MHC/peptide tetramers underscore the critical role of tumor antigen presentation and the context in which it occurs. They indicate that antigen presentation only by activated but not by cancer or resting dendritic cells is necessary for the induction of immune responses to tumor antigens. It becomes apparent that the inability of dendritic cells to become activated provides a biological niche for tumor escape from immune destruction and seems to be a principal mechanism for the failure of tumor immune surveillance.
Comparative analysis of prostatic acid phosphatase and prostate-specific antigen mRNA levels in hyperplastic prostate stimulated with steroid hormones and growth factors.
Authors: Dulińska Joanna , Laidler Piotr , Labedź Maria
Published: December 2019

Prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) are the markers of human prostatic gland. However, it is still not completely understood if and how, steroid hormones and growth factors affect their expression and metabolism in the respect to the major pathologies of the gland. Appropriate studies were carried out on histopathologically diagnosed benign prostatic hyperplasia--BPH (n = 42) using tissue slices and cells derived from them. They were incubated with steroid hormones: 5-alpha-dihydrotestosterone (DHT), estradiol (E) and growth factors: epidermal growth factor (EGF), basic fibroblastic growth factor (bFGF) under culture conditions for up to 24 hours. P-labelled specific oligonucleotide probes were used to analyze total RNA isolated from each sample for the presence of PAP and PSA mRNAs. DHT, E, bFGF, EGF or both DHT + bFGF and DHT + EGF increased PAP and PSA mRNA levels in a time- and dose-dependent manner. The highest and statistically significant increase (P < 0.001) for PAP mRNA was observed when DHT + bFGF were present in the medium while for PSA mRNA if DHT + EGF were added to the medium. Slow but constant decrease of PAP and PSA mRNA levels was observed in the absence of each of these factors in the incubation medium. The results suggest that early expression of PSA and PAP genes and/or their mRNA stability strongly depend on DHT while differ in their response to EGF and bFGF.
Additional copies of the NOG2 and IST2 genes suppress the deficiency of cohesin Irr1p/Scc3p in Saccharomyces cerevisiae.
Authors: Białkowska Agnieszka , Kurlandzka Anna
Published: December 2019

The protein encoded by the IRR1/SCC3 gene is an element of the cohesin complex of Saccharomyces cerevisiae, responsible for establishing and maintaining sister chromatid cohesion during mitotic cell division. We noticed previously that lowering the level of expression of IRR1/SCC3 affects colony formation on solid support. Here we describe two dosage suppressors (IST2, NOG2) overcoming the inability to form colonies of an Irr1p-deficient strain. Ist2 is probably involved in osmotolerance, Nog2p is a putative GTPase required for 60S ribosomal subunit maturation, but may also participate in mRNA splicing.
Collagen of umbilical cord vein and its alterations in pre-eclampsia.
Authors: Romanowicz Lech , Jaworski Stefan
Published: December 2019

The state of the vascular system of the mother and of placenta is known to exert a great influence on intrauterinal development of the fetus. Pre-eclampsia is the most common pathological syndrome connected with pregnancy. Since collagen is one of the main constituents of the vessel wall a comparison was made with collagen content and its molecular polymorphism in umbilical cord veins of newborns from healthy and pre-eclamptic mothers. It was found that umbilical cord veins of newborns from mothers with pre-eclampsia contained 18% less collagen than those of the newborns from normal pregnancies. This decrease was accompanied by a slight decrease of collagen solubility, but all its types (I, II, IV, V and VI) were present. However, the umbilical vein wall of newborns from mothers with pre-eclampsia contained relatively less of type I and more of type III collagen than the normal umbilical cord. These differences may be connected with a disturbance of blood flow in fetus of a woman with pre-eclampsia.
Spectrophotometric method for the determination of renal ouabain-sensitive H+,K+-ATPase activity.
Authors: Bełtowski Jerzy , Wójcicka Grazyna
Published: December 2019

The aim of this work was to develop a method for renal H+,K+-ATPase measurement based on the previously used Na+,K+-ATPase assay (Beltowski et al.: J Physiol Pharmacol.; 1998, 49: 625-37). ATPase activity was assessed by measuring the amount of inorganic phosphate liberated from ATP by isolated microsomal fraction. Both ouabain-sensitive and ouabain-resistant K+-stimulated and Na+-independent ATPase activity was detected in the renal cortex and medulla. These activities were blocked by 0.2 mM imidazolpyridine derivative, Sch 28080. The method for ouabain-sensitive H+,K+-ATPase assay is characterized by good reproducibility, linearity and recovery. In contrast, the assay for ouabain-resistant H+,K+-ATPase was unsatisfactory, probably due to low activity of this enzyme. Ouabain-sensitive H+,K+-ATPase was stimulated by K+ with Km of 0.26 +/- 0.04 mM and 0.69 +/- 0.11 mM in cortex and medulla, respectively, and was inhibited by ouabain (Ki of 2.9 +/- 0.3 microM in the renal cortex and 1.9 +/- 0.4 microM in the renal medulla) and by Sch 28080 (Ki of 1.8 +/- 0.5 microM and 2.5 +/- 0.9 microM in cortex and medulla, respectively). We found that ouabain-sensitive H+,K+-ATPase accounted for about 12% of total ouabain-sensitive activity in the Na+,K+-ATPase assay. Therefore, we suggest to use Sch 28080 during Na+,K+-ATPase measurement to block H+,K+-ATPase and improve the assay specificity. Leptin administered intraperitoneally (1 mg/kg) decreased renal medullary Na+,K+-ATPase activity by 32.1% at 1 h after injection but had no effect on H+,K+-ATPase activity suggesting that the two renal ouabain-sensitive ATPases are separately regulated.
Cloning and characterization of the gene encoding ribosomal P0 phosphoprotein from Neurospora crassa.
Authors: Krokowski Dawid , Tchórzewski Marek , Grankowski Nikodem
Published: December 2019

A gene for ribosomal protein P0 that belongs to the family of ribosomal P proteins was isolated from a Neurospora crassa cDNA library, using polyclonal antibodies against recombinant P0 protein from Saccharomyces cerevisiae. This is the first gene for ribosomal P0 protein to be cloned from filamentous fungi. The derived P0 protein sequence has a strong homology to other eukaryotic P0 proteins; yet, there is a notable alteration in the conservative C-terminal region, placing this protein among the unique sequences from protozoan parasites.
Yellow lupine gene encoding stearoyl-ACP desaturase--organization, expression and potential application.
Authors: Zaborowska Zaneta , Starzycki Michał , Femiak Iwona , Swiderski Michał , Legocki Andrzej B
Published: December 2019

A gene for the delta9 desaturase specific to stearoyl-ACP (acyl carrier protein) was identified from yellow lupine (Lupinus luteus) cDNA and genomic libraries through the differential display method. The desaturase transcript appears in plants infected with Bradyrhizobium sp. (Lupinus) as revealed by Northern hybridization, RT-PCR and expression of beta-glucuronidase under the desaturase promoter. A small amount of desaturase transcript was also detected in uninfected plants, which suggests that the gene does not belong to the strict nodule-specific sequences. The desaturase provides unsaturated fatty acids for additional cell membrane synthesis. During nodule and symbiosome development a peribacteroid membrane is formed and the requirement for membrane surface increases, thus the level of desaturase expression is also higher. Transgenic plants of Nicotiana tabacum with overexpression of the full-length lupine stearoyl-ACP desaturase sequence were obtained. They revealed higher content of unsaturated fatty acids (especially oleic acid) in comparison with control plants.
The ability to overcome multidrug resistance of tumor cell lines by novel acridine cytostatics with condensed heterocyclic rings.
Authors: Bontemps-Gracz Maria M , Kupiec Agnieszka , Antonini Ippolito , Borowski Edward
Published: December 2019

Two recently synthesized groups of acridine cytostatics containing fused heterocyclic ring(s): pyrazoloacridines (PAC) and pyrazolopyrimidoacridines (PPAC) were tested in regard to their in vitro cytotoxic activity towards a panel of sensitive and resistant human tumor cell lines. The obtained results corroborate our earlier hypothesis on the essential role of heterocyclic ring fused to the acridine moiety in the ability of acridine cytostatics to overcome multidrug resistance of tumor cells. The presence, location and kind of substituents considerably influenced both the cytotoxic activity of the derivatives and their ability to overcome multidrug resistance. The same factors also affected the cytostatics ability to differentiate between tumor cell lines with various types of drug exporting pumps.
Cross-resistance to five glucocorticoids in childhood acute lymphoblastic and non-lymphoblastic leukemia samples tested by the MTT assay: preliminary report.
Authors: Styczyński Jan , Wysocki Mariusz , Debski Robert , Balwierz Walentyna , Rokicka-Milewska Roma , Matysiak Michał , Balcerska Anna , Kowalczyk Jerzy , Wachowiak Jacek , Sońta-Jakimczyk Danuta , Chybicka Alicja
Published: December 2019

In vitro antileukemic activity of five glucocorticoids and their cross-resistance pattern in childhood acute lymphoblastic and non-lymphoblastic leukemia were determined by means of the MTT assay in 25 leukemia cell samples of childhood acute leukemias. The equivalent antileukemic concentrations of the drugs tested were: 34 microM hydrocortisone (HC), 8 microM prednisolone (PRE), 1.5 microM methylprednisolone (MPR), 0.44 microM dexamethasone (DX) and 0.22 microM betamethasone (BET). In comparison with initial ALL cell samples, the relapsed ALL group was more resistant to PRE (38-fold, p = 0.044), DX (> 34-fold, p = 0.04), MPR (38-fold), BET (45-fold) and HC (33-fold). The AML cell samples were even more resistant to: PRE (> 85-fold, p = 0.001), DX (> 34-fold, p = 0.004), MPR (> 69-fold, p = 0.036), BET (> 69-fold, p = 0.038) and HC (54-fold, p = 0.059) when compared with ALL on initial diagnosis. A significant cross-resistance among all the glucocorticoids used was found. Only in some individual cases the cross-resistance was less pronounced.
TEL/JAK2 tyrosine kinase inhibits DNA repair in the presence of amifostine.
Authors: Gloc Ewa , Warszawski Mariusz , Młynarski Wojciech , Stolarska Małgorzata , Hoser Grazyna , Skorski Tomasz , Błasiak Janusz
Published: December 2019

The TEL/JAK2 chromosomal translocation (t(9;12)(p24;p13)) is associated with T cell childhood acute lymphoblastic leukemia. The TEL/JAK2 fusion protein contains the JAK2 catalytic domain and the TEL-specific oligomerization domain. TEL-mediated oligomerization of the TEL/JAK2 proteins results in the constitutive activation of the tyrosine kinase activity. Leukemia cells expressing TEL/JAK2 tyrosine kinase become resistant to anti-neoplastic drugs. Amifostine is a pro-drug which can selectively protect normal tissues against the toxicity of anticancer drugs and radiation. We investigated the effects of amifostine on idarubicin-induced DNA damage and repair in murine pro-B lymphoid BaF3 cells and BaF3-TEL/JAK2-transformed cells using alkaline single cell gel electrophoresis (comet assay). Idarubicin induced DNA damage in both cell types but amifostine reduced its extent in control non-transformed BaF3 cells and enhanced it in TEL/JAK2-transformed cells. The transformed cells did not show measurable DNA repair after exposure to amifostine and idarubicin, but cells treated only with idarubicin were able to recover within a 60-min incubation. Because TEL/JAK2-transformed cells can be considered as model cells for certain human leukemias and lymphomas we anticipate an enhancement of idarubicin cytotoxicity by amifostine in these diseases. Moreover, TEL/JAK2 tyrosine kinase might be involved in cellular response to DNA damage. Amifostine could promote apoptosis or lower the threshold for apoptosis induction dependent on TEL/JAK2 activation.
Isophosphoramide mustard analogues as prodrugs for anticancer gene-directed enzyme-prodrug therapy (GDEPT).
Authors: Misiura Konrad , Szymanowicz Daria , Kuśnierczyk Halina , Wietrzyk Joanna , Opolski Adam
Published: December 2019

Two types of prodrugs, benzyl analogues of isophosphoramide mustard (iPAM), activated by cytochrome P450, and acylthioethyl analogues, activated by esterases, were designed. In contrast to iPAM that hydrolyse rapidly, the examined compounds are stable in phosphate-buffered saline and Tris buffer. Benzyl analogues of iPAM are poor substrates for cytochrome P450, are not cytotoxic and posses no antitumour activity. Acylthioethyl analogues of iPAM are good substrates for pig liver esterase, are cytotoxic and exert antitumour activity against L1210 leukaemia in mice. The observed correlation for iPAM analogues between their susceptibility to hydrolysis and cytotoxicity and antitumour activity suggests possible application of these compounds as the prodrugs in gene-directed enzyme-prodrug therapy.
In vitro release of cytotoxic nucleoside analogs from lactide-caprolactone and lactide-glycolide copolymers.
Authors: Kryczka Tomasz , Bero Maciej , Kasperczyk Janusz , Dobrzyński Piotr , Marciniec Barbara , Popielarz-Brzezińska Maria , Grieb Pawel
Published: December 2019

The aims of our study were to assess the release of cytotoxic nucleoside analogs 5-fluorouracil and 2-chloro-2'-deoxyadenosine from different lactide-glycolide or lactide-caprolactone biodegradable copolymers and the effects of sterilization on this release. The polymers were sterilized either with ethylene oxide at 37 degrees C, or with gamma radiation (15 kGy, 20 kGy, or 25 kGy). The kinetics of nucleoside release from the copolymers were measured over 50 days. Four copolymers exhibited relatively constant release of nucleosides in micromolar concentrations during the entire observation period. Sterilization with either ethylene oxide or gamma radiation only slightly influenced nucleoside release. Further development of these copolymers as an intracerebral nucleoside delivery system for local treatment of brain tumors is indicated.
In vitro activity of oxazaphosphorines in childhood acute leukemia: preliminary report.
Authors: Styczyński Jan , Wysocki Mariusz , Debski Robert , Balwierz Walentyna , Rokicka-Milewska Roma , Matysiak Michał , Balcerska Anna , Kowalczyk Jerzy , Wachowiak Jacek , Sońta-Jakimczyk Danuta , Chybicka Alicja
Published: December 2019

Glufosfamide (beta-D-glucosyl-ifosfamide mustard) is a new agent for cancer chemotherapy. Its pharmacology is similar to commonly used oxazaphosphorines, but it does not require activation by hepatic cytochrome P-450 and preclinically demonstrates lower nephrotoxicity and myelosuppression than ifosfamide. The aim of the study was a comparison of the drug resistance profiles of glufosfamide and other oxazaphosphorines in childhood acute leukemias. Leukemic cells, taken from children with ALL on diagnosis (n = 41), ALL on relapse (n = 12) and AML on diagnosis (n = 13) were analyzed by means of the MTT assay. The following drugs were tested: glufosfamide (GLU), 4-HOO-ifosfamide (IFO), 4-HOO-cyclophosphamide (CYC) and mafosfamide cyclohexylamine salt (MAF). In the group of initial ALL samples median cytotoxicity values for GLU, IFO, CYC and MAF were 15.5, 33.8, 15.7 and 7.8 microM, respectively. In comparison with initial ALL samples, the relative resistance for GLU and IFO in relapsed ALL samples was 1.9 (p = 0.049) and 1.3 (ns), and in initial AML samples 31 (p < 0.001) and 5 (p = 0.001), respectively. All oxazaphosphorines presented highly significant cross-resistance. Glufosfamide presented high activity against lymphoblasts both on diagnosis and on relapse.
Effect of cyclosporin A on immunological response in lungs of guinea pigs infected with Trichinella spiralis.
Authors: Dzik Jolanta M , Zieliński Zbigniew , Gołos Barbara , Jagielska Elzbieta , Wranicz Mariusz , Wałajtys-Rode Elzbieta
Published: December 2019

The effects of cyclosporin A (CsA), a potent immunosuppressive drug with antiparasitic activity, on the innate immunological response in guinea pig lungs during an early period (6th and 14th days) after T. spiralis infection were studied. CsA treatment of T. spiralis-infected guinea pigs caused a significant attenuation of immunological response in lungs by decreasing lymphocyte infiltration into pulmonary alveolar space, inhibiting alveolar macrophage superoxide anion production and lowering both the production of NO metabolites measured in bronchoalveolar lavage fluid and expression of the iNOS protein in lung homogenates, allowing us to speculate that the T. spiralis-dependent immunological response is dependent on lymphocyte T function. Interestingly, CsA itself had a pro-inflammatory effect, promoting leucocyte accumulation and macrophage superoxide production in guinea pig lungs. This observation may have a relevance to the situation in patients undergoing CsA therapy. Macrophage expression of the iNOS protein, evaluated by immunoblotting was not influenced by treatment of animals with CsA or anti-TGF-antibody, indicating different regulation of the guinea pig and murine enzymes.
Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.
Authors: Kit Yuri , Kuligina Elena , Semenov Dimitry , Richter Vladimir
Published: December 2019

Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [gamma-32P]ATP. The greatest was the incorporation of P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.
The influence of a single and double biotinylation of xanthohumol on its anticancer activity
Authors: Stompor Monika , Świtalska Marta , Wietrzyk Joanna
Published: December 2019

Two biotinylated derivatives of the main hop chalcone xanthohumol (1) were prepared by a one-step synthesis via esterification using biotin and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC×HCl) and 4-dimethylaminopyridine (DMAP) as coupling reagents. The products were characterized spectroscopically and their antiproliferative activity toward MCF-7, MCF-10A, HepG2, MDA-MB-231, 4T1 and Balb/3T3 cell lines was investigated using the SRB assay. For all three tested compounds the best activity was noted in the case of human (MCF-7) and mice (4T1) breast cancer cell lines (IC50 values < 9 μM). Both biotinylated derivatives showed slightly higher anticancer activity than xanthohumol (1) towards all types of tested breast cancer cells. Double biotinylated xanthohumol (3) proved to be the most active in inhibiting cell growth, with IC50 values equal to 5.35 ± 1.5 μM for 4T1 and 8.03 ± 0.53 µM for MCF-7 cell lines. Compound 3 was also more active than 1 and 2 against liver cancer cells HepG2 (IC50 = 17.37 ± 5.1 μM), while the IC50 values for 1 and 2 were equal to 21.5 ± 2.7 and 22.1 ± 3.9 µM, respectively. 4‑O‑biotinylxanthohumol (2) was the second most active growth inhibitor, particularly with respect to MCF-7 (IC50 = 6.19 ± 1.7 μM) and 4T1 (IC50 = 6.64 ± 0.4 μM) cell lines. Our preliminary study on biotinylated xanthohumol (1) have shown that this type of functionalization is an effective method for the production of active biomolecules and study on this area should be continued thereby extending their applications.
La vulnerabilidad del sector artesanal colombiano en el ámbito nacional e internacional como ‘negocio de conocimiento tradicional’. Estudio de caso: la denominación de origen artesanal ‘Guacamayas’ en el Acuerdo Comercial entre Colombia, Perú y la Unión E
Authors: Manquillo Astaíza Natalia Andrea
Published: December 2019

Las artesanías pueden considerarse como un símbolo de conservación cultural y como un negocio en el comercio exterior. Este articulo propone entender la artesanía como un negocio de conocimiento tradicional. Para ello, la primera parte del texto abordara las características del sector artesanal colombiano y algunos factores que lo afectan en el ámbito nacional e internacional. La segunda parte se encargará de reconstruir el problema sobre la desprotección jurídica de la denominación de origen artesanal Guacamayas en el Acuerdo Comercial entre Colombia, Perú y la Unión Europea. Aquí se hallará una de las principales razones de la vulnerabilidad del sector artesanal: la desprotección jurídica en el campo de la propiedad intelectual. La conclusión de este texto mostrara que solo cuando sean superados los imaginarios sociales errados, suplidas las necesidades de las comunidades artesanales y se fortalezca el derecho a la propiedad intelectual sobre las artesanías de cara al Derecho internacional, se podrán garantizar la conservación del legado cultural y el crecimiento de un sector de la economía nacional.
Big data: un análisis documental de su uso y aplicación en el contexto de la era digital
Authors: Escobar Borja Mariana , Mercado Pérez Margareth
Published: December 2019

El Big Data es un sistema de procesamiento de datos a través de las TIC, caracterizado por variabilidad, velocidad y volumen. Esta investigación analiza su uso y aplicación en el contexto de la era digital, utilizando una revisión teórica. Como resultados, el Big Data aporta: celeridad en la gestión de información, apoyo para sistemas económicos, financieros y productivos y, fundamento a decisiones sobre aspectos sociales. Como reflexión, los sectores sociales deben invertir en tic que les permitan sacar provecho de los datos; en el ámbito investigativo, el Big Data debe conceptualizarse claramente, ya que este es difuso en el entorno teórico analizado.
Patrimonialización, desarrollo territorial y nuevos modelos de gobernanza. El caso del ramal ferroviario Talca-Constitución en la región del Maule, Chile
Authors: Leiva Cañete Fabiola , Díaz Meeks Pablo
Published: December 2019

A partir de la caracterización del caso del ramal Talca-Constitución, monumento his­tórico propiedad de una empresa pública de gestión autónoma y último ramal ferroviario en funcionamiento en Chile, el artículo expon­drá algunas reflexiones en torno a la multidi­mensionalidad que deben asumir los procesos de patrimonialización en contextos donde conviven expresiones culturales y naturales únicas con dinámicas de pobreza, exclusión, desigualdad y riesgo ambiental. También se discutirán los aportes que en este escenario tienen las miradas que buscan armonizar los procesos socioculturales de reivindicación y apropiación del territorio y su dotación de recursos bioculturales, con el diseño de estra­tegias de desarrollo territorial que colaboren con el bienestar y la calidad de vida de las co­munidades. Finalmente, se abordará la necesi­dad de imaginar y testear nuevos acuerdos de gobernanza y sistemas de gestión que se hagan cargo de la complejidad territorial, por un lado, y, por otro, puedan responder a los nuevos es­tándares de participación que diversos grupos sociales exigen en la actualidad.
Quelle collaboration public-privé pour l’intégration d’un projet minier à son territoire? Études de cas en Afrique de l'Ouest
Authors: Rey Pascal , Mazalto Marie
Published: December 2019

Le boom de l’exploitation minière en Afrique a soulevé de nombreuses questions à propos du développement régional dans les zones minières et les synergies entre les secteurs privé et public. La position adoptée par la société minière et les États et les inégalités entre les différents acteurs participent à créer le flou dans la rela­tion public-privé et sa perception. À travers des travaux de recherche et d’ex­pertise réalisés entre 2008 et 2016 en Afrique de l’Ouest, nous proposons de réexaminer les rôles et les responsabilités des parties prenantes dans la création de synergies entre les secteurs privé et public. Nous explorerons les stratégies déployées des deux côtés pour comprendre les intérêts qui influencent la mise en oeuvre des politiques.
Participación electoral en el Magdalena Medio colombiano. Un análisis del posacuerdo con las FARC
Authors: Barrios Flor Manuelita
Published: December 2019

La región del Magdalena Medio colom­biano es uno de los territorios mayormente afectados por el control político y social que ejercieron las Fuerzas Armadas Revolucionarias de Colombia (Farc), en especial en los muni­cipios ubicados en la periferia, caracterizados por estar en zonas de difícil acceso y con poca presencia estatal. En este sentido, en este docu­mento se contrasta el comportamiento político durante la refrendación de los Acuerdos de Paz con las elecciones al Senado de la República de 2018 –en las que las Farc por primera vez participaron como partido político–. Esto con el fin de analizar la configuración política antes y después de la implementación de los acuerdos, así como su incidencia en la región del Magdalena Medio, en comparación con el resto del país.
Method of computing hydraulic gradient for the case of a two-phase mixture flowing in sloping pipes
Authors: Kondratiev A.S. , Nha T.L.
Published: December 2019

The paper investigates two-phase mixture flows in sloping pipes employing two computational methods in the transitional region of pipe slope angle. We used methods of computing two-mixture flows in horizontal and vertical pipes as the basis for our equations. When flowing downwards through a sloping pipe, the solid phase volume ratio distribution changes most significantly: an axisymmetric flow through a vertical pipe transforms into a flow featuring a markedly non-uniform distribution of the solid phase along the vertical plane in the sloping pipe. When flowing upwards, the solid phase volume ratio profile is inversely transformed. Comparison of the experimental and computational data showed that the datasets are in a sufficiently good agreement. The computational method developed is semi-empirical and may be recommended for calculating hydraulic gradient in sloping pipes.
QUEM SÃO OS ATUAIS ESTUDANTES DAS LICENCIATURAS NO BRASIL? Perfil socioeconômico e relação com o magistério
Authors: Locatelli Cleomar , Diniz-Pereira Júlio Emílio
Published: December 2019

O artigo analisa o perfil dos estudantes de licenciatura no Brasil, considerando, especialmente, as condições socioeconômicas e a relação com o magistério. O objetivo é relacionar informações atuais referentes aos estudantes de licenciaturas, avaliando o contexto do trabalho docente e a formação inicial de professores. A pesquisa reuniu dados junto a cinco licenciaturas de todo o país: pedagogia, história, matemática, educação física e biologia, a partir das respostas dadas ao questionário do estudante do Exame Nacional do Desempenho do Estudante (Enade/2014-2017). Constata-se um perfil de estudante trabalhador, com renda familiar baixa, egresso de escola pública e que, em sua maioria, afirma ter escolhido o magistério pela vocação ou pela importância da profissão.WHO ARE THE TEACHER EDUCATION STUDENTS IN BRAZIL TODAY? socioeconomic profile and their relationship with teachingAbstractThis article analyzes the profile of undergraduate students from teacher education programs in Brazil, considering their socioeconomic conditions and the relationship with the teaching profession. The objective is to relate current information regarding undergraduate students, assessing the context of teaching work and initial teacher education.  The survey gathered data from five undergraduate degrees across the country: pedagogy, history, mathematics, physical education and biology, from the replies to the questionnaire of the National Survey of Student Performance (Enade/2014-2017). We can observe a profile of a student worker, with a low family income, who graduated from a public school and who, for the most part, claims to have chosen the magisterium by vocation or importance of the profession.Keywords: teacher education; teacher education programs; students’ profile.¿QUIÉNES SON LOS ACTUALES ESTUDIANTES DE LICENCIA EN BRASIL? Perfil socioeconómico y relación con la profesión docenteResumenEl artículo analiza el perfil de los estudiantes de licenciatura en Brasil, considerando, especialmente, las condiciones socioeconómicas y la relación con el magisterio. El objetivo es relacionar informaciones actuales referentes a los estudiantes de licenciaturas, evaluando el contexto del trabajo docente y la formación inicial de profesores. La encuesta reunió datos junto a cinco licenciaturas en todo el país: pedagogía, historia, matemáticas, educación física y biología, a partir de las respuestas dadas al cuestionario del estudiante del Examen Nacional del Desempeño del Estudiante (Enade / 2014-2017). Se constata un perfil de estudiante trabajador, con renta familiar baja, egresado de escuela pública y que, en su mayoría, afirma haber escogido el magisterio por la vocación o por la importancia de la profesión.Palabras clave: formación de profesores; licenciaturas, perfil de los estudiantes.
KAJIAN SEAKEEPING KAPAL FERRY RO-RO 750 GT BERBASIS PENGUJIAN HIDRODINAMIKA
Authors: Ahmad Mujahid Syaiful
Published: December 2019

The Ferry Ro-Ro is a ship that functions to crossing inter island and to transport vehicles, passengers and goods. Building a ship requires a very large investment value so that the design of the ship is a very important consideration by conduct the intens study to determine the performance of a ship, one of them is the performance of the ship motion, endurance, and reliability while sailing which can be seen by conducting a hydrodynamic test in the form of a seakeeping test on the Ferry Ro-Ro Ship Model. This study conduct the seakeeping testing due to Ferry Ro-Ro 750 GT Ship Model which aims to predict the pattern of ship behavior by using the seakeeping test methods at Manoeuvering and Ocean Basin at Laboratorium Hidrodinamika Indonesia at Balai Teknologi Hirdoinamika - BPPT with th parameter Wave Height Hs = 2.50 m, Wave Period Tp = 9.00 s by heading 180 deg and 135 deg, and model scale is 1 : 21.19. The seakeeping test procedure for the Ferry Ro-Ro 750 GT Ship Model is based on ITTC No 7.5-02-07-02 standards. Testing of the ship model is conducted by using the method of free running where ship moving at a speed of 12 knots (actual scale). Analysis of measurement results is displayed in the form of Response Amplitude Operator (RAO) and statical analysis. The hydrodynamic test results in the form of a seakeeping test of Ferry Ro-Ro 750 GT Ship Model shows the value of Root Mean Square (RMS) of each direction of the ship relative to the direction of arrival of the wave (heading) 180 deg with roll value = 0.825 deg and pitch value = 2.231 deg. And heading 135 deg with roll value = 2.410 deg and pitch value = 1.797 deg, where NORDFORSK 1987 criteria standard for RMS roll value is 6 deg, and RMS pitch value is 1.5 deg.

Keywords: Seakeeping, Ferry Ro-Ro, Hydrodynamics
A proximidade de um parque paleontológico estimulando o conhecimento entre estudantes da educação básica brasileira
Authors: Oliveira Guilherme Cordeiro da Graça de , Oliva Edwaldo , Ribeiro Fátima Sueli Neto , Balbino Ausenda Cáceres , Castro Luiz Otávio Rezende
Published: December 2019

Este trabalho aborda o ensino de Paleontologia na Educação Básica Brasileira. O objetivo principal do estudo foi investigar se a proximidade da escola de um parque paleontológico - com todas as possibilidades educativas que daí possa advir - poderia resultar em maiores conhecimentos sobre paleontologia. A metodologia empregada caracteriza-se como uma pesquisa levantamento quantitativa e exploratória. Foram aplicados questionários sobre conhecimentos básicos e os resultados obtidos sugerem que a proximidade do parque influenciou nos conhecimentos paleontológicos dos estudantes. São discutidas e sugeridas formas de utilização do parque com um efetivo espaço não formal para o ensino e aprendizagem de conceitos paleontológicos.
Política de estágio da Universidade Federal de Mato Grosso e a transição escola-trabalho
Authors: Kuhn Caiubi Emanuel Souza , Guimarães Paulo Henrique Monteiro
Published: December 2019

A transição escola-trabalho é uma preocupação cada vez maior de diversos governos no mundo, isso por que os maiores índices de desemprego em geral estão associados com o público jovem, ademais, a forma como essa transição ocorre pode determinar a condição social do indivíduo ao longo da vida. Nessa perspectiva, este estudo realizou uma análise das políticas de estágio, nos cursos de engenharia da Universidade Federal de Mato Grosso, sobre a ótica do processo de transição escola-trabalho. Para tanto, foi realizada uma análise documental dos projetos pedagógicos de curso. Os resultados demonstram que vários cursos possuem restrições que impedem a realização de estágio nos períodos iniciais, fato que pode favorecer a evasão escolar, assim como, amplia a dificuldade de estudantes em situação de vulnerabilidade econômica de acenderem no nível acadêmico.