bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Tables S1-S2, Figures S1-S6 from Pharmaceutical Reactivation of Attenuated Apoptotic Pathways Leads to Elimination of Osimertinib Drug-Tolerant Cells
Authors: Martin Matthew J. , Floc'h Nicolas , Pfeifer Matthias , Criscione Steven , Delpuech Oona , Gagrica Sladjana , Yao Yi , McDermott Ultan , Smith Paul D.
Published: April 2023

Supplementary Table 1. Cell lines used. Supplementary Table 2. Antibodies used. Supplementary Figure S1. Osimertinib drug tolerant cells re-aquire apoptotic capacity after drug holiday. Supplementary Figure S2. BH3 mimetics can trigger apoptosis in established DTPs. Supplementary Figure S3. DTPs upregulate cIAP proteins and are sensitive to SMAC mimetic treatment. Supplementary Figure S4. SMAC mimetics inhibit DTP growth in vivo. Supplementary Figure S5. BH3 and SMAC mimetics enhance apoptosis induced by agents targeting resistance drivers. Supplementary Figure S6. Measuring transcript levels for anti-apoptotic genes in osimertinib DTPs.
Figure S5 from Impaired Antitumor Immune Response in MYCN-amplified Neuroblastoma Is Associated with Lack of CCL2 Secretion and Poor Dendritic Cell Recruitment
Authors: Kacher Jamila , Manches Olivier , Aspord Caroline , Sartelet Hervé , Chaperot Laurence
Published: April 2023

Supplementary figure 5 shows analysis of MYCN level in NB tumors in GSE62564 according to MYCN amplification status
Figure S4 from Impaired Antitumor Immune Response in MYCN-amplified Neuroblastoma Is Associated with Lack of CCL2 Secretion and Poor Dendritic Cell Recruitment
Authors: Kacher Jamila , Manches Olivier , Aspord Caroline , Sartelet Hervé , Chaperot Laurence
Published: April 2023

Supplementary figure 4 shows flow cytometry gating strategy for cell subsets identification (migration experiments)
Supplementary Figure S3 from A Novel PHD2/VHL-mediated Regulation of YAP1 Contributes to VEGF Expression and Angiogenesis
Authors: Bora-Singhal Namrata , Saha Biswarup , Mohankumar Durairaj , Padmanabhan Jaya , Coppola Domenico , Chellappan Srikumar
Published: April 2023

Downregulation of VEGF mRNA when YAP1 is depleted
Supplementary Material S8 from Diagnostic Accuracy of Blood-based Biomarkers for Pancreatic Cancer: A Systematic Review and Meta-analysis
Authors: Kane Laura E. , Mellotte Gregory S. , Mylod Eimear , O'Brien Rebecca M. , O'Connell Fiona , Buckley Croí E. , Arlow Jennifer , Nguyen Khanh , Mockler David , Meade Aidan D. , Ryan Barbara M. , Maher Stephen G.
Published: April 2023

Figure S8 panels A-E
Supplementary Material S6 from Diagnostic Accuracy of Blood-based Biomarkers for Pancreatic Cancer: A Systematic Review and Meta-analysis
Authors: Kane Laura E. , Mellotte Gregory S. , Mylod Eimear , O'Brien Rebecca M. , O'Connell Fiona , Buckley Croí E. , Arlow Jennifer , Nguyen Khanh , Mockler David , Meade Aidan D. , Ryan Barbara M. , Maher Stephen G.
Published: April 2023

QUADAS-2
Supplementary Material S5 from Diagnostic Accuracy of Blood-based Biomarkers for Pancreatic Cancer: A Systematic Review and Meta-analysis
Authors: Kane Laura E. , Mellotte Gregory S. , Mylod Eimear , O'Brien Rebecca M. , O'Connell Fiona , Buckley Croí E. , Arlow Jennifer , Nguyen Khanh , Mockler David , Meade Aidan D. , Ryan Barbara M. , Maher Stephen G.
Published: April 2023

Complete list of extracted data fields
Figure S2 from CD47-blocking Antibody ZL-1201 Promotes Tumor-associated Macrophage Phagocytic Activity and Enhances the Efficacy of the Therapeutic Antibodies and Chemotherapy
Authors: Cao Anthony , Yi Jiaqing , Tang Xinyan , Szeto Christopher W. , Wu Renyi , Wan Bing , Fang Xu , Li Shou , Wang Lei , Wang Lina , Li Jing , Ye Qiuping , Huang Tom , Hsu Karl , Kabbarah Omar , Zhou Haiying
Published: April 2023

CD47 expression and phagocytosis by ZL-1201
Table TS2 from Loss of the Volume-regulated Anion Channel Components LRRC8A and LRRC8D Limits Platinum Drug Efficacy
Authors: Widmer Carmen A. , Klebic Ismar , Domanitskaya Natalya , Decollogny Morgane , Howald Denise , Siffert Myriam , Essers Paul , Nowicka Zuzanna , Stokar-Regenscheit Nadine , van de Ven Marieke , de Korte-Grimmerink Renske , Galván José A. , Pritchard Colin E.J. , Huijbers Ivo J. , Fendler Wojciech , Vens Conchita , Rottenberg Sven
Published: April 2023

Primer sequences used for Lrrc8a or Lrrc8d knockout control in 2D cell lines or Lrrc8d knockout mice or reconstitution experiments
Figure FS5 from Loss of the Volume-regulated Anion Channel Components LRRC8A and LRRC8D Limits Platinum Drug Efficacy
Authors: Widmer Carmen A. , Klebic Ismar , Domanitskaya Natalya , Decollogny Morgane , Howald Denise , Siffert Myriam , Essers Paul , Nowicka Zuzanna , Stokar-Regenscheit Nadine , van de Ven Marieke , de Korte-Grimmerink Renske , Galván José A. , Pritchard Colin E.J. , Huijbers Ivo J. , Fendler Wojciech , Vens Conchita , Rottenberg Sven
Published: April 2023

Lrrc8d KO mice tolerate higher cisplatin doses than wild type mice
Figure FS1 from Loss of the Volume-regulated Anion Channel Components LRRC8A and LRRC8D Limits Platinum Drug Efficacy
Authors: Widmer Carmen A. , Klebic Ismar , Domanitskaya Natalya , Decollogny Morgane , Howald Denise , Siffert Myriam , Essers Paul , Nowicka Zuzanna , Stokar-Regenscheit Nadine , van de Ven Marieke , de Korte-Grimmerink Renske , Galván José A. , Pritchard Colin E.J. , Huijbers Ivo J. , Fendler Wojciech , Vens Conchita , Rottenberg Sven
Published: April 2023

Generation of monoclonal knockout cell lines of Lrrc8a or Lrrc8d used in main Figure
Supplementary Figure 9 from Remodeling of the Tumor Microenvironment Through PAK4 Inhibition Sensitizes Tumors to Immune Checkpoint Blockade
Authors: Abril-Rodriguez Gabriel , Torrejon Davis Y. , Karin Daniel , Campbell Katie M. , Medina Egmidio , Saco Justin D. , Galvez Mildred , Champhekar Ameya S. , Perez-Garcilazo Ivan , Baselga-Carretero Ignacio , Singh Jas , Comin-Anduix Begoña , Puig-Saus Cristina , Ribas Antoni
Published: April 2023

Supplementary Figure 9: PAK4 inhibitor, A0317859, also synergizes with PD-1 blockade in YUMM2.1 melanoma cells.
Supplementary Table ST3 from Antibody Binding and Neutralization of Live SARS-CoV-2 Variants Including BA.4/5 Following Booster Vaccination of Patients with B-cell Malignancies
Authors: Chang Andres , Akhtar Akil , Lai Lilin , Orellana-Noia Victor M. , Linderman Susanne L. , McCook-Veal Ashley A. , Switchenko Jeffrey M. , Saini Manpreet , Valanparambil Rajesh M. , Blum Kristie A. , Allen Pamela B. , Lechowicz Mary Jo , Romancik Jason T. , Ayers Amy , Leal Alyssa , O'Leary Colin B. , Churnetski Michael C. , Baird Katelin , Kives Melissa , Wrammert Jens , Nooka Ajay K. , Koff Jean L. , Dhodapkar Madhav V. , Suthar Mehul S. , Cohen Jonathon B. , Ahmed Rafi
Published: April 2023

Supplementary Table 3. Univariate analyses of clinical variables associated with booster response
Figure S1 from MEK5-ERK5 Axis Promotes Self-renewal and Tumorigenicity of Glioma Stem Cells
Authors: Fukasawa Kazuya , Lyu Jiajun , Kubo Takuya , Tanaka Yuki , Suzuki Akane , Horie Tetsuhiro , Tomizawa Akane , Osumi Ryoma , Iwahashi Sayuki , Tokumura Kazuya , Murata Misato , Kobayashi Masaki , Todo Tomoki , Hirao Atsushi , Hinoi Eiichi
Published: April 2023

Figure S1
Table TS3 from Misaligned Chromosomes are a Major Source of Chromosomal Instability in Breast Cancer
Authors: Tucker John B. , Bonema Sarah C. , García-Varela Rebeca , Denu Ryan A. , Hu Yang , McGregor Stephanie M. , Burkard Mark E. , Weaver Beth A.
Published: April 2023

Table S3. Results from multivariable regression, related to Figure 3.
Supplementary Figure 3 from Endothelial VEGFR Coreceptors Neuropilin-1 and Neuropilin-2 Are Essential for Tumor Angiogenesis
Authors: Benwell Christopher J. , Johnson Robert T. , Taylor James A.G.E. , Price Christopher A. , Robinson Stephen D.
Published: April 2023

NRP co-depletion promotes VEGF receptor degradation
Figure S7 from Loss of Dok-3 in Non-tumor Cells Induces Malignant Transformation of Benign Epithelial Tumor Cells of the Intestine
Authors: Arimura Sumimasa , Inoue-Yamauchi Akane , Katayama Kotoe , Kanno Tatsuo , Jozawa Hiroki , Imoto Seiya , Yamanashi Yuji
Published: April 2023

mRNA expression of Dok-1 and Dok-2, but not Dok-3, is detectable in tumor epithelial cells.
Figure S3 from Loss of Dok-3 in Non-tumor Cells Induces Malignant Transformation of Benign Epithelial Tumor Cells of the Intestine
Authors: Arimura Sumimasa , Inoue-Yamauchi Akane , Katayama Kotoe , Kanno Tatsuo , Jozawa Hiroki , Imoto Seiya , Yamanashi Yuji
Published: April 2023

Assessment of invasion depth.
Figure S1 from Loss of Dok-3 in Non-tumor Cells Induces Malignant Transformation of Benign Epithelial Tumor Cells of the Intestine
Authors: Arimura Sumimasa , Inoue-Yamauchi Akane , Katayama Kotoe , Kanno Tatsuo , Jozawa Hiroki , Imoto Seiya , Yamanashi Yuji
Published: April 2023

Loss of Dok-1/-2 enhances intestinal tumor growth in Apc mice.
Supplementary Table ST1 from Targeting MLL Methyltransferases Enhances the Antitumor Effects of PI3K Inhibition in Hormone Receptor–positive Breast Cancer
Authors: Jones Robert B. , Farhi Jonathan , Adams Miranda , Parwani Kiran K. , Cooper Garrett W. , Zecevic Milica , Lee Richard S. , Hong Andrew L. , Spangle Jennifer M.
Published: April 2023

Supplementary Table 1 shows p values for IHC staining that corresponds to the graphs shown in Figure 5D
Figure S4 from Targeting MLL Methyltransferases Enhances the Antitumor Effects of PI3K Inhibition in Hormone Receptor–positive Breast Cancer
Authors: Jones Robert B. , Farhi Jonathan , Adams Miranda , Parwani Kiran K. , Cooper Garrett W. , Zecevic Milica , Lee Richard S. , Hong Andrew L. , Spangle Jennifer M.
Published: April 2023

Figure S4 shows combined PI3K and MLL1 inhibition reduces cell viability and enhances apoptosis
Table S5 from Elevated Dietary Carbohydrate and Glycemic Intake Associate with an Altered Oral Microbial Ecosystem in Two Large U.S. Cohorts
Authors: Monson Kelsey R. , Peters Brandilyn A. , Usyk Mykhaylo , Um Caroline Y. , Oberstein Paul E. , McCullough Marjorie L. , Purdue Mark P. , Freedman Neal D. , Hayes Richard B. , Ahn Jiyoung
Published: April 2023

Sensitivity analyses, taxon abundance
Table S2 from Elevated Dietary Carbohydrate and Glycemic Intake Associate with an Altered Oral Microbial Ecosystem in Two Large U.S. Cohorts
Authors: Monson Kelsey R. , Peters Brandilyn A. , Usyk Mykhaylo , Um Caroline Y. , Oberstein Paul E. , McCullough Marjorie L. , Purdue Mark P. , Freedman Neal D. , Hayes Richard B. , Ahn Jiyoung
Published: April 2023

Carbohydrate and GI Beta diversity
Figure S7 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells
Authors: Milutinovic Stefan , Abe Jun , Jones Emma , Kelch Inken , Smart Kathryn , Lauder Sarah N. , Somerville Michelle , Ware Carl , Godkin Andrew , Stein Jens V. , Bogle Gib , Gallimore Awen
Published: April 2023

Impact of Treg depletion and LTβR agonist treatment on the immune cell organisation in fibrosarcoma tumours.
Figure S6 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells
Authors: Milutinovic Stefan , Abe Jun , Jones Emma , Kelch Inken , Smart Kathryn , Lauder Sarah N. , Somerville Michelle , Ware Carl , Godkin Andrew , Stein Jens V. , Bogle Gib , Gallimore Awen
Published: April 2023

Global overview of intra-tumoral HEVs in 4T1 tumours following PI-3065 treatment.
Figure S5 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells
Authors: Milutinovic Stefan , Abe Jun , Jones Emma , Kelch Inken , Smart Kathryn , Lauder Sarah N. , Somerville Michelle , Ware Carl , Godkin Andrew , Stein Jens V. , Bogle Gib , Gallimore Awen
Published: April 2023

LSFM imaging of Treg depleted and T-cell depleted fibrosarcomas.
Figure S3 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells
Authors: Milutinovic Stefan , Abe Jun , Jones Emma , Kelch Inken , Smart Kathryn , Lauder Sarah N. , Somerville Michelle , Ware Carl , Godkin Andrew , Stein Jens V. , Bogle Gib , Gallimore Awen
Published: April 2023

Impact of Treg depletion on LN HEV remodelling
Supplementary Figure S3 from Targeting NEDDylation is a Novel Strategy to Attenuate Cisplatin-induced Nephrotoxicity
Authors: Jones Trace M. , Espitia Claudia M. , Chipollini Juan , Lee Benjamin R. , Wertheim Jason A. , Carew Jennifer S. , Nawrocki Steffan T.
Published: April 2023

Knockdown of NRF2 blunts pevonedistat-mediated protection from cisplatin.
Supplementary Figure S1 from The GPER Agonist LNS8801 Induces Mitotic Arrest and Apoptosis in Uveal Melanoma Cells
Authors: Ambrosini Grazia , Natale Christopher A. , Musi Elgilda , Garyantes Tina , Schwartz Gary K.
Published: April 2023

The mitotic effect of LNS8801 is GPER-independent
PSReliP: an integrated pipeline for analysis and visualization of population structure and relatedness based on genome-wide genetic variant data
Authors: Solovieva Elena , Sakai Hiroaki
Published: April 2023

Background

Population structure and cryptic relatedness between individuals (samples) are two major factors affecting false positives in genome-wide association studies (GWAS). In addition, population stratification and genetic relatedness in genomic selection in animal and plant breeding can affect prediction accuracy. The methods commonly used for solving these problems are principal component analysis (to adjust for population stratification) and marker-based kinship estimates (to correct for the confounding effects of genetic relatedness). Currently, many tools and software are available that analyze genetic variation among individuals to determine population structure and genetic relationships. However, none of these tools or pipelines perform such analyses in a single workflow and visualize all the various results in a single interactive web application.

Results

We developed PSReliP, a standalone, freely available pipeline for the analysis and visualization of population structure and relatedness between individuals in a user-specified genetic variant dataset. The analysis stage of PSReliP is responsible for executing all steps of data filtering and analysis and contains an ordered sequence of commands from PLINK, a whole-genome association analysis toolset, along with in-house shell scripts and Perl programs that support data pipelining. The visualization stage is provided by Shiny apps, an R-based interactive web application. In this study, we describe the characteristics and features of PSReliP and demonstrate how it can be applied to real genome-wide genetic variant data.

Conclusions

The PSReliP pipeline allows users to quickly analyze genetic variants such as single nucleotide polymorphisms and small insertions or deletions at the genome level to estimate population structure and cryptic relatedness using PLINK software and to visualize the analysis results in interactive tables, plots, and charts using Shiny technology. The analysis and assessment of population stratification and genetic relatedness can aid in choosing an appropriate approach for the statistical analysis of GWAS data and predictions in genomic selection. The various outputs from PLINK can be used for further downstream analysis. The code and manual for PSReliP are available at https://github.com/solelena/PSReliP.
Are medical students happy despite unhappy conditions: a qualitative exploration of medical student cohorts during disruptive conditions
Authors: Esguerra Stephen , Chiu Fiona Thuy , Espinoza Alyssa , Williams Dan , Clithero-Eridon Amy
Published: April 2023

Background

Shortly after the World Health Organization declared the SARS-CoV-2 outbreak a worldwide pandemic, medical school governing bodies issued guidance recommending pausing clinical rotations. Prior to the availability of COVD-19 vaccines, many schools implemented exclusively online curriculums in the didactic and clinical years. These unprecedented events and paradigm changes in medical education could contribute to trainee burnout, wellness, and mental health.

Methods

This single-institution study interviewed first, second, and third-year medical students from a medical school in the southwestern United States. A semi-structured interview was conducted with paper-based Likert scale questions rating perceived happiness were administered both at the time of the interview and one year later in order to understand how their student experience and happiness were impacted. In addition, we asked participants to describe any major life events they experienced since the first interview.

Results

Twenty-seven volunteers participated in the original interview. Twenty-four from the original cohort participated in the one-year follow-up. Happiness as a sense of self and who you “should be” was challenged during the pandemic and changes in happiness over time were not systematic across classes. Stress was caused not only by the pandemic which was experienced by all, but by a tripartite state of individual circumstances, academic workload requirements, and the world at large. Primary themes from the interviews were clustered around the individual, learner, and future professional levels and focused on the primacy of relationships, emotional wellness, stress management, professional identity, and impacts of educational disruptions. These themes created risk factors for developing imposter syndrome. Students demonstrated resiliency across cohorts and were able to utilize a variety of strategies to achieve and maintain both physical and mental health, but the primacy of relationships both personally and professionally was noted.

Conclusion

Medical students’ identities as individual persons, a learner, and future medical professionals were all impacted by the pandemic. The results from this study suggest that the COVID-19 pandemic and changes in the learning format and environment may create a new risk factor in the development of imposter syndrome. There is also an opportunity to re-consider resources to achieve and maintain wellness during a disrupted academic environment.
Application of computed tomography-guided hook-wire localization technique in thoracoscopic surgery for small pulmonary nodules (≤ 10 mm)
Authors: Yang Yuan , Qin Chenhui , Ma Yue , Lu Zhongting , Zhang Yun , Li Tao
Published: April 2023

Objective

This study aimed to investigate the safety and efficacy of the computed tomography (CT)-guided hook-wire localization technique in thoracoscopic surgery for small pulmonary nodules (≤ 10 mm) and to identify the risk factors for localization-related complications.

Methods

The medical records of 150 patients with small pulmonary nodules treated from January 2018 to June 2021 were retrospectively analyzed. According to preoperative hook-wire positioning status, they were divided into the localization group (50 cases) or the control group (100 cases). The operation time, intraoperative blood loss, hospital stay, and conversion rate to thoracotomy were recorded and compared between groups. Uni- and multivariate binary logistic regression analysis was used to identify the risk factors for localization-related complications.

Results

A total of 58 nodules were localized in 50 patients in the localization group, and the localization success rate was 98.3% (57/58). In one case, the positioning pin fell off before wedge resection was performed. The mean nodule diameter was 7.05 mm (range, 2.8–10.0 mm), while the mean depth from the pleura was 22.40 mm (range, 5.47–79.47 mm). There were 8 cases (16%) of asymptomatic pneumothorax, 2 (4%) of intrapulmonary hemorrhage, and 1 (2%) of pleural reaction.The mean operation time of the localization group (103.88 ± 41.74 min) was significantly shorter than that of the control group (133.30 ± 45.42 min) (P < 0.05). The mean intraoperative blood loss of the localization group (44.20 ± 34.17 mL) was significantly lower than that of the control group (112.30 ± 219.90 mL) (P < 0.05). The mean hospital stay of the localization group (7.96 ± 2.34 days) was significantly shorter than that of the control group (9.21 ± 3.25 days).Multivariate binary logistic analysis showed that localization times of small pulmonary nodules in the localization group was an independent risk factor for localization-related pneumothorax.

Conclusions

Our results suggest that the CT-guided hook-wire localization technique is beneficial for localizing small pulmonary nodules. Specifically, it is helpful for the diagnosis and treatment of early lung cancer because it can accurately remove lesions, decrease intraoperative blood loss, shorten operation time and hospitalization stay, and reduce thoracotomy conversion rate. Simultaneous positioning of multiple nodules can easily lead to positioning-related pneumothorax.
Dihydroartemisinin-piperaquine or sulphadoxine-pyrimethamine for the chemoprevention of malaria in children with sickle cell anaemia in eastern and southern Africa (CHEMCHA): a protocol for a multi-centre, two-arm, double-blind, randomised, placebo-controlled superiority trial
Authors: Nkosi-Gondwe Thandile , Robberstad Bjarne , Opoka Robert , Kalibbala Dennis , Rujumba Joseph , Galileya Lufina Tsirizani , Akun Pamela , Nambatya Winnie , Ssenkusu John , TerKuile Feiko , Phiri Kamija , Idro Richard
Published: April 2023

Background

An estimated 300,000 babies are born with sickle cell anaemia (SCA) annually. Affected children have chronic ill health and suffer premature death. Febrile illnesses such as malaria commonly precipitate acute crises in children with SCA. Thus, chemoprophylaxis for malaria is an important preventive strategy, but current regimes are either sub-optimally effective (e.g. monthly sulphadoxine-pyrimethamine, SP) or difficult to adhere to (e.g. daily proguanil). We propose dihydroartemisinin-piperaquine (DP) as the agent with the most potential to be used across Africa.

Methods

This will be a randomised, double-blind, parallel-group superiority trial of weekly single-day courses of DP compared to monthly single-day courses of SP in children with SCA. The study will be conducted in eastern (Uganda) and southern (Malawi) Africa using randomisation stratified by body weight and study centre. Participants will be randomised using an allocation of 1:1 to DP or SP. We will investigate the efficacy, safety, acceptability and uptake and cost-effectiveness of malaria chemoprevention with weekly courses of DP vs monthly SP in 548 to 824 children with SCA followed up for 12–18 months. We will also assess toxicity from cumulative DP dosing and the development of resistance. Participant recruitment commenced on 30 April 2021; follow-up is ongoing.

Discussion

At the end of this study, findings will be used to inform regional health policy. This manuscript is prepared from protocol version 2.1 dated 1 January 2022.

Trial registration

The trial was registered at ClinicalTrials.gov, NCT04844099. Registered on 08 April 2021.
The Right to Need-Satisfaction
Authors: Elder-Woodward Jim
Published: April 2023

This article argues that a ‘Right to Need-Satisfaction’ should be the basis of the Scottish Government’s proposed National Care Service. This service will take a ‘human rights-based approach’. The premise of this right is: if people are to participate in society, then they must be given sufficient resources to do so. However, this stance is insufficient unless such resources are accompanied by an awareness of people’s own oppressed situation within society and the support people need from their peer group.
Scaling Comb-Driven Resonator-Based DWDM Silicon Photonic Links to Multi-Tb/s in the Multi-FSR Regime
Authors: James Aneek , Novick Asher , Rizzo Anthony , Parsons Robert , Jang Kaylx , Hattink Maarten , Bergman Keren
Published: April 2023

The use of chip-based micro-resonator Kerr frequency combs in conjunction with dense wavelength-division multiplexing (DWDM) enables massively parallel intensity-modulated direct-detection (IM-DD) data transmission with low energy consumption. Resonator-based modulators and filters used in such systems can limit the number of usable wavelength channels due to practical constraints on the maximum achievable free spectral range (FSR). In this work, we introduce the design of multi-Tb/s comb-driven resonator-based silicon photonic links by leveraging the multi-FSR regime. We demonstrate the viability of the link architecture with yield estimates that are supported by extensive wafer-scale measurements of 704 micro-resonators fabricated in a commercial complementary metal–oxide–semiconductor (CMOS) foundry. We show that a 2.80 Tb/s link is realizable with a ≥ 6σ yield (~99.999%), and aggregate bandwidths of 3.76 Tb/s and 4.72 Tb/s are possible if yield targets are relaxed (3σ and 1σ respectively). All designs represent a 1.94—3.28× boost to aggregate link bandwidth while maintaining BER ≤ 10−10 performance, with a theoretical bandwidth of 10.51 Tb/s being possible for sufficiently robust resonators. We use high-speed BER measurements to inform co-optimization of data rate and aggressor spacing (λag), limiting any additional loss-based power penalties to off-resonance insertion loss (IL) and routing loss. This work demonstrates that, through the multi-FSR regime, there is a clear path toward Kerr comb-driven ultra-broadband, high bandwidth silicon photonic links that can support next-generation data centers and high performance computers.
A qualitative insight into researchers’ perceptions of gender inequality in medical and dental research institutions in Nigeria
Authors: Folayan Morenike Oluwatoyin , Olowokeere Adekemi , Lusher Joanne , Aina Olabisi , Gascon Ana , Martínez-Pérez Guillermo Z.
Published: April 2023

Objective

The aim of the study was to gain a qualitative insight into scientific researchers’ perceptions of gender inequality inside Nigerian research institutions through an investigation of how gender equality is enacted in medical and dental research institutions in Nigeria.

Methods

This descriptive and cross-sectional qualitative study probed decision-making around navigating gender inequity and explored opinions about how a supportive environment for female medical and dental researchers could be established. Data were collected through semi-structured telephone interviews with 54 scientific researchers across 17 medical and dental academic institutions in Nigeria between March and July 2022. Data were transcribed verbatim and analyzed using thematic analysis.

Results

Three core themes emerged: institutionalized male dominance in research institutions; changing narratives on gender equalities in research and academic enterprise; and women driving the conscience for change in research institutions. Female medical and dental researchers’ perceived gender equality was challenging mainstream androcentric values in knowledge production within the medical and dental field; and queries the entrenchment of patriarchal values that promote a low number of female medical and dental trainees, fewer female research outputs, and few women in senior/managerial positions in the medical fields.

Conclusion

Despite the general view that change is occurring, a great deal remains to be done to facilitate the creation of a supportive environment for female medical and dental researchers in Nigeria.
Transformation of forest governance: policy concepts and actor changes in social forestry program implementation in Indonesia
Authors: Ramadhan R. , Dhavio M. , Hanafi R. , Daulay M.H. , Prakosa G.G.
Published: April 2023

In Indonesia, particularly in Java, forest governance has been taking place since the State Forest Corporation (SFC) came to power. The emergence of the Social Forestry Forest Utilization Permit (IPHPS) and Strengthening and Protection-Forestry Partnership (Kulin-KK) programs in the
SFC area marked the start of an essential phase of social forestry (SF), in which forest access and management were transferred to community groups. The 2017 regulatory policy regarding SF programs in the SFC region significantly impacts governance and actor relations in Java. This study examines
the transformation that is taking place using the concept of policy arrangement and analysis of changes in actors at the program implementation level. We find that policy coalitions are formed by government actors and civil society groups using forest and socioeconomic condition-based discourse.
The rules of the game that are formed cannot be divorced from the interests of the actors who desire change. The findings at IPHPS indicate that SFC actors have less impact than they do in the Kulin-KK program, where they remain powerful because this program is regarded as a continuation of
the PHBM concept that SFC introduced. Community group facilitators are influential actors in a network of community groups in Java that are mainly involved in promoting social forestry programs. This study proposes expanding community participation in the program so that previously powerful
actors' interests do not exploit it.
An intersectional analysis of women's experiences in forest carbon monitoring in Peru, Cameroon and Nepal
Authors: McConnell A.E. , Schenkein S.F. , Nuñez-Iturri G. , Gurung D. , Siwe R. , Leahy J.E. , Kenefic L.S. , Wilson S. , Straub C.L.
Published: April 2023

Gender equity has progressed unequally within science, technology, engineering and mathematics (STEM) fields, with geosciences and specifically the Earth Observation (EO) field remaining largely unbalanced. Limited work has centred women's experiences in forest carbon monitoring (FCM),
a field that combines EO and in situ data collection. Fewer studies have focused on perceptions of women from Latin America, Africa and Asia. This study examines participants in SilvaCarbon, an interagency technical cooperation program of the U.S. Government to build FCM capacity in
tropical countries, where fewer women have historically participated as participants or training leaders. To better understand barriers that women face in FCM, we analyse personal narratives shared by women in Peru, Cameroon and Nepal. The framework of intersectionality facilitates exploration
of intersectional identities in concert with gender that affect women in three distinct country contexts. Findings indicate that individuals' opportunities in FCM are affected by multiple combined identities and circumstances at the personal and structural levels, and through socially constructed,
attributed, and perceived identities.
Evaluation of DNA barcodes in discriminating Quercus species from Lam Dong, Vietnam
Authors: Van Ngoc Nguyen , Van Duy Nong , Minh Phuong Nguyen Thi , Binh Hoang Thi
Published: April 2023

DNA barcoding is a tool for species discrimination and identification which helps overcome the problem of identification that cannot be covered by morphological identification. Quercus is the second biggest genus of Fagaceae in Vietnam as well as Lam Dong province after Lithocarpus. However, the species discrimination study of the Quercus species in Vietnam and Lam Dong province has yet to be well uncovered due to ambiguous species boundaries and the lack of universal molecular markers. In this study, the DNA barcodes were tested to discriminate among the species of the Quercus genus in Lam Dong province. A total of sixteen and two samples of the Quercus and Lithocarpus genus (out-group) were tested using matK, rbcL, and internal transcribed spacer (ITS). Of which, the new sequences in this study were sequenced from six species and one unknown species of Quercus, the rest was retrieved from GenBank. The BLAST, neighbor-joining, and Bayesian methods were employed to examine species discrimination success. The results showed that ITS was an efficient single-locus barcode for Quercus species by yielding the highest rate of universality as well as the best discriminatory and authentication power among the barcodes examined. In addition, the combination of ITS+matK+rbcL achieved the highest species discrimination. Therefore, matK and rbcL should not be used as DNA barcodes for the species identification of Quercus, whereas the combination of three genes that were proposed in this study is the most suitable DNA barcode for identifying Quercus species in Lam Dong province.
The Effect of Physical Activity on State of Mind: The Mediating Role of Mental Resilience during the COVID-19 Pandemic
Authors: Mi Xuan , Zhang Meng , Zhang Guoli
Published: April 2023

To investigate the current state of home-based physical activity during the COVID-19 pandemic and to explore the effects of physical activity on state of mind and the mediating role of mental resilience, we selected five hundred people from across China to fill out the relevant scales by a convenience sampling method. The data were statistically analyzed using SPSS 22.0 and Amos 22.0. This study found that residents living at home during the COVID-19 pandemic reported reduced physical activity. Home-based physical activity can improve the state of mind directly and improve it indirectly by influencing mental resilience. Residents are advised to participate in physical activity when they stay at home, as doing so benefits their physical and mental health.
Evaluation of associations between TP53 codon 72 polymorphism and onset and progression of oral potentially malignant disorders based on case-control studies
Authors: Zhou Shanxin , Liu Yu , Yang Xi , Zhou Qin
Published: April 2023

Background
Recently, a systematic review and meta-analysis demonstrated that overexpression of p53 immunoprotein was significantly associated with progression risk of oral potentially malignant disorders (OPMD). However, the results of investigations on TP53 genetic typing in OPMD were inconsistent and inconclusive.
Methods
A systematic evaluation was conducted to identify all eligible case-control studies on the associations between TP53 codon 72 polymorphism and both onset and progression of OPMD.
Results
A total of 768 OPMD patients and 1173 healthy individuals were identified from 12 eligible case-control studies on TP53 codon 72 polymorphism OPMD onset. In overall and subgroup analyses, no significantly risk of OPMD onset was observed in the cases for genetic models including allele C vs. G, homozygote CC vs. GG, heterozygote GC vs. GG, dominant GC + CC vs. GG, and recessive CC vs. GG + GC (all P-value of association test > 0.05). Further, a total of 465 OPMD patients and 775 oral squamous cell carcinoma (OSCC) ones were identified from 8 eligible case-control studies on this polymorphism in OPMD progression to OSCC. The analyses revealed that there was also no significantly risk of OPMD progression in the cases for the genetic models (all P-value of association test > 0.05).
Conclusion
Our data of a pooled-analysis indicates that TP53 codon 72 polymorphism may not act as genetic factor for the risk of OPMD onset and progression. Combined with the conclusion by a systematic review and meta-analysis, we put forward a new opinion that TP53 genetic typing cloud not influence p53 protein expression in OPMD.
P53 upregulation by USP7-engaging molecular glues
Authors: Lu Boxun , Li Zhaoyang , Wang Ziying , An Ping , Zhang Hang , Zhong Chao , Liu Rui , Ma Zhiqiang , Lu Junmei , Pan Chengfang , Xing Dong , Fei Yiyan , Ding Yu
Published: April 2023

Molecular glues are typically small chemical molecules that act on the interface between the target protein and the degradation machinery to trigger ternary complex formation. Identification of molecular glues is challenging, and there has been a lack of target-upregulating molecular glues, which are desired for many targets such as tumor suppressor proteins (TSPs). TSPs are usually degraded by the proteasome through polyubiquitination (poly-ub) by specific E3 ligases, whereas deubiquitinases (DUBs) are capable of removing poly-ub conjugates to counteract these E3 ligases. Thus, small molecular glues that enhance the anchoring of TSPs to DUBs may stabilize them through deubiquitination. Here, through small-molecule microarray-based technology and unbiased screening, we identified three potential molecular glues that may tether P53 to the DUB USP7 and elevate the P53 level. Among them, bromocriptine (BC) is an FDA-approved drug showing the most robust effects. We further demonstrated that BC increased P53 stability via the predicted molecular glue mechanism engaging USP7. To confirm the generality of the screening platform, we identified another USP7-engaging molecular glue that upregulates PTEN, which is another well-known TSP. Taken together, we established a potential screening platform that may facilitate the discovery of novel molecular glues stabilizing TSPs via engaging the DUB USP7. Similar strategies could be applied to the identification of other types of molecular glues that may benefit drug discovery and chemical biology studies.
Earthquake Source Parameters in Zagros Region (Iran) From the Time -Evolutive P-wave Displacement
Authors: Nazeri Sahar , Abdi Fatemeh , Ismail Amir , Rahimi Habib , Zollo Aldo
Published: April 2023

The rupture process of the recent moderate-to-large earthquakes in the Zagros area along the Iran plateau is investigated by analysing the strong motion data provided by the Iranian Building and Housing Research Centre (BHRC). The selected dataset includes the largest and deadliest 2017 Mw 7.3, Iran-Iraq (Ezgeleh) earthquake. The earthquake source parameters (moment magnitude, rupture duration and length, average slip, and static stress drop) are determined using a time-domain, parametric modelling technique based on the time evolution of the P-wave displacement signals. The earthquake source parameters are calculated from simulated triangular moment-rate functions assuming the circular source models for a constant rupture velocity. The anelastic attenuation effect is modelled through the independent frequency-Q parameter ranging from 50 to 200 and accounted for by a post-processing procedure that retrieves the attenuation-corrected, moment-rate triangular shape. Results show that the average static stress-drop with different \({Q}_{P}\), varies between < Δ𝜎>=0.9 (0.7 − 1.2) MPa and < Δ𝜎>=1.6 (1.2 − 2.0) MPa. Assuming a circular rupture model for Ezgeleh earthquake, we estimate a moment magnitude of 6.9, rupture duration of 7 seconds, source radius of 16 km, average slip of about 2 m and static stress drop of 3.4 MPa.
Can a bulky glycocalyx promote catch bonding in early integrin adhesion? Perhaps a bit
Authors: Blanchard Aaron
Published: April 2023

Many types of cancer overexpress bulky glycoproteins to form a thick glycocalyx layer. The glycocalyx physically separates the cell from its surroundings, but recent work has shown that the glycocalyx can paradoxically increase adhesion to soft tissues and therefore promote the metastasis of cancer cells. This surprising phenomenon occurs because the glycocalyx forces adhesion molecules (called integrins) on the cell’s surface into clusters. These integrin clusters have cooperative effects that allow them to form stronger adhesions to surrounding tissues than would be possible with equivalent numbers of un-clustered integrins. These cooperative mechanisms have been intensely scrutinized in recent years; a more nuanced understanding of the biophysical underpinnings of glycocalyx-mediated adhesion could uncover therapeutic targets, deepen our general understanding of cancer metastasis, and elucidate general biophysical processes that extend far beyond the realm of cancer research. This work examines the hypothesis that the glycocalyx has the additional effect of increasing mechanical tension experienced by clustered integrins. Integrins function as mechanosensors that undergo catch bonding – meaning the application of moderate tension increases integrin bond lifetime relative to the lifetime of integrins experiencing low tension. In this work, a three-state chemomechanical catch bond model of integrin tension is used to investigate catch bonding in the presence of a bulky glycocalyx. This modeling suggests that a bulky glycocalyx can lightly trigger catch bonding, increasing the bond lifetime of integrins at adhesion edges by up to 100%. The total number of integrin-ligand bonds within an adhesion is predicted to increase by up to ~ 60% for certain adhesion geometries. Catch bonding is predicted to decrease the activation energy of adhesion formation by ~ 1–4 kBT, which translates to a ~ 3–50× increase in the kinetic rate of adhesion nucleation. This work reveals that integrin mechanic and clustering likely both contribute to glycocalyx-mediated metastasis.
Flexible surface-enhanced Raman scattering strips using colloidal ink of gold- silver alloyed nanoparticles
Authors: Tian Honghao , Qin Youjian , Liu Hongmei , Li Tian , Li Yuting , Fang Xiaohui , Zhang Xinping
Published: April 2023

Surface-enhanced Raman scattering (SERS) has been used for trace detection at the single-molecule level. The low-cost preparation of high-performance test strips has enabled the development of SERS techniques. In this study, oil-dispersible metal or alloy nanoparticles prepared by the Brust-Schiffrin method were used as "inks" in a ballpoint pen to handwrite SERS test strips on polytetrafluoroethylene (PTFE) membranes. Because of the good PTFE lipophilicity, the flexible substrates had good uniformity. The large laser damage threshold of the PTFE membrane also enabled increased laser powers for SERS testing. The Au and Ag alloy nanoparticle inks exhibited increased performance with larger proportions of Ag. The Au1Ag8 nanoparticles had the best properties, and those strips could detect 10-11-M Rhodamine 6G dyes in a 5-µL volume with an enhancement factor of 5.4×108. The SERS strips were used to demonstrate detection of malachite green, the use of which is prohibited in aquaculture and fish tanks.
Synthesis Cobalt Complexed Single Chain Polymer and its Nanographene-based Composites, Electrical, Optical, and Thermal properties
Authors: Zebari Osama Ismail Haji , DEMİRELLİ Kadir , Zeebaree Samie Yaseen Sharaf , Tuncer Hülya
Published: April 2023

Atom transfer radical polymerization (ATRP) technique was used in the preparation of Poly [4-((4-vinylbenzyl)oxy) phthalonitrile-co-methyl methacrylate] P(VBOPN 13.4%- co-MMA) at a temperature of 110oC. Single chain polymer complexed cobalt phthalocyanine (SCP-CoPc) have been synthesized via cyclotautomerization approach using aforementioned copolymer caring VBOPN pendant group to form green colored single chain folded polymer molecule at a high range of temperature 140-150oC and high dilution condition of cyclohexanol solvent. The formation of cobalt atom collapsed via intramolecular forces into single chain polymer was characterized by Fourier-transform infrared spectroscopy and UV/Vis spectroscopy and shows high solubility in most organic solvents. The thermal of SCP-CoPc and nanogaphene-based SCP-CoPc/NG 6 wt% nanocomposite as well as electrical, dielectric, and optical properties of both materials were investigated. SCP-CoPc/NG 6 wt% nanocomposite was observed to have temperature-dependent increases in dielectric and dc. electrical conductivity. The activation energy, Ea was found to be 0.295 eV for the I. region and 0.39 eV for the II region, demonstrating that the conduction followed the Arrhenius equation. Estimates were made regarding the optical constants of SCP-CoPc/NG 6 wt%, specifically the refractive index, the real value of the optical dielectric constant, and the optical band gap. Both the direct band gap and the refractive index of SCP-CoPc and SCP-CoPc/NG 6 wt% were calculated, and the direct band gap of SCP-CoPc was calculated to be 2.83 eV, while the direct band gap of SCP-CoPc/NG 6 wt% was calculated to be 2.58 eV.
Efficacy of the peritoneovenous shunt as palliative care for cancer patients with refractory ascites: a randomized controlled trial (JIVROSG-0803 study)
Authors: Arai Yasuaki , Sone Miyuki , Takeuchi Yoshito , Sugawara Shunsuke , Aramaki Takeshi , Tanaka Toshihiro , Sato Mikio , Morita Shinichi
Published: April 2023

Background
A randomized, controlled trial to evaluate the superiority of peritoneovenous shunt (PVS) over the other treatments for refractory ascites in advanced cancer patient was conducted.
Subjects and methods
The subjects were advanced cancer patients with symptoms due to refractory ascites which are difficult to control by medical therapy and cannot be improved by methods other than drainage and with a Palliative Prognostic Index of <6. They were randomly allocated in a 1:1 ratio to receive either PVS placement (test group) or the other treatment (control group). The area under the curve (AUC) of symptom improvement score curve calculated with visual analog scale was compared between the groups as the primary endpoint. The AUC of EQ-5D, SF-8 were also compared between the groups, and adverse events were evaluated with the CTCAE version 3 up to 4 weeks after enrollment or until protocol treatment was discontinued.
Results
Forty patients were enrolled between November 2009 and July 2018, with 20 allocated to test group and 20 control group. The mean AUC of the symptom improvement score curves were 133.2 (95% CI: 98.6-167.7) in the test group and 69.9 (95% CI: 34.4-105.4) in the control group (p=0.014). Therefore, the mean AUC was significantly larger in the test group, resulting the primary endpoint was met. There was no significant difference between the groups in SF-8 and EQ-5D. Though some known adverse events were observed, there was no significant difference in the frequency of individual adverse events between the groups.
Conclusions
PVS significantly improves the symptom of pain due to refractory ascites compared to other treatments. Although not as effective as improving the comprehensive QOL evaluated with SF-8 and EQ-5D, PVS may be considered the standard of care for advanced cancer patients with refractory ascites.
Clinical value of circulating tumor cells and hematological parameters in 617 Chinese patients with colorectal cancer: Retrospective analysis
Authors: He Yuhao , He Xinxin , Zhou Yubo , Luo Shanshan
Published: April 2023

Background:Circulating tumor cells (CTCs) was a popular non-invasive liquid biopsy technique in recent years. The purpose of this study was to investigate the relationship between circulating tumor cells and colorectal cancer.
Methods: The clinical data of 617 patients with colorectal cancer from October 2019 to March 2022 were retrospectively collected to analyze the correlation between CTCs and clinicopathologic characteristics.
Results: The CTCs value increased with the progression of T stage, M stage and TNM stage (P < 0.05), but had no correlation with N stage (P > 0.05). Binary logistic regression analysis showed that CTCs, CEA, CA125 and CA199 were independent risk factors for distant CRC metastasis. Compared with CTCs, CEA, CA125 and CA199, the Logistic model had the highest AUC (AUC=0.778,95%CI: 0.732-0.824), and the specificity and sensitivity were 82.9% and 63.2%, respectively. After operation, chemoradiotherapy and other treatment for CRC, CTCs and CEA were significantly decreased compared with before treatment (P < 0.05). In addition, Spearman Correlation showed significant correlation between CTCs and IgG (P=0.000).
Conclusion: CTCs was closely related to the stage of colorectal cancer, the prediction of distant organ metastasis, and the evaluation of therapeutic effect, which is helpful for clinical decision-making.