bluuebunny/crossref_metadata_2025 huggingface dataset:
Garlicnin B1, an Active Cyclic Sulfide from Garlic, Exhibits Potent Anti-Inflammatory and Anti-Tumor Activities
Authors: Gao Shanghui , Yang Kai , Nohara Toshihiro , Ikeda Tsuyoshi , Zhou Jian-Rong , Yokomizo Kazumi , Fang Jun
Published: April 2023

This study aimed to investigate the pharmacological activities of garlicnin B1, a cyclic sulfide compound found abundantly in garlic and structurally similar to onionin A1, which has been shown to possess strong anti-tumor effects. In vitro studies demonstrated that garlicnin B1 significantly reduced intracellular reactive oxygen species triggered by hydrogen peroxide in colon cancer cells. In a mouse colitis model induced by dextran sulfate sodium, garlicnin B1 at a low dose (5 mg/kg) remarkably ameliorated the symptoms and pathological progression. Additionally, garlicnin B1 exhibited considerable tumoricidal activity with an IC50 value of ~20 μM, as observed in cytotoxicity assays. In vivo experiments using the mouse sarcoma S180 transplanted model and the azoxymethane (AOM) or DSS-induced colon cancer model showed that garlicnin B1 effectively suppressed tumor growth in a dose-dependent manner, with marked inhibition at 80 mg/kg. These results suggest that garlicnin B1 has diverse functions that could be achieved by carefully manipulating the dosing regimen. We anticipate that garlicnin B1 has the potential to be used beneficially in the future for the treatment of cancer and inflammatory diseases, although further studies are warranted to elucidate its mechanisms of action.
Hypoxia Enhances Oxidative Stress in Neutrophils from ZZ Alpha-1 Antitrypsin Deficiency Patients
Authors: Magallón María , Castillo-Corullón Silvia , Bañuls Lucía , Pellicer Daniel , Romero Teresa , Martínez-Ferraro Carlos , Navarro-García María Mercedes , Herrejón Alberto , González Cruz , Dasí Francisco
Published: April 2023

Alpha-1 antitrypsin deficiency (AATD) is a neutrophilic inflammatory disorder that may result in local hypoxia, reactive oxygen and nitrogen species (ROS/RNS) production, and increased damage in adjacent tissues. This study aims to determine the impact of hypoxia on neutrophil oxidative stress profile in AATD patients. Neutrophils were isolated from AATD patients and control volunteers and exposed to hypoxia (1% O2 for 4 h), ROS/RNS, mitochondrial parameters, and non-enzymatic antioxidant defenses measured by flow cytometry. The expression of enzymatic antioxidant defenses was determined by qRT-PCR. Our results indicate that ZZ-AATD neutrophils produce higher amounts of hydrogen peroxide, peroxynitrite, and nitric oxide and decreased levels of the antioxidant enzymes catalase, superoxide dismutase, and glutathione reductase. Likewise, our results show a decrease in mitochondrial membrane potential, indicating that this organelle could be involved in the production of the reactive species observed. No decrease in glutathione and thiol levels were observed. The accumulation of substances with high oxidative capacity would explain the greater oxidative damage observed in proteins and lipids. In conclusion, our results indicate that, compared to MM control individuals, ZZ-AATD neutrophils show increased ROS/RNS production under hypoxic conditions opening a new rationale for using antioxidant therapies to treat the disease.
Essential Oils in Postharvest Treatment against Microbial Spoilage of the Rosaceae Family Fruits
Authors: Magri Anna , Curci Martina , Battaglia Valerio , Fiorentino Antonio , Petriccione Milena
Published: April 2023

Fruits undergo numerous chemical, physical, and microbiological changes during storage that shorten their postharvest life, reducing shelf-life and boosting food loss. Food quality and safety are seriously threatened by postharvest infections, one of the factors behind postharvest deterioration and mycotoxin contamination in fruits. The control of postharvest deterioration is a big concern because there are few management methods available. Several attempts have been undertaken to prevent the microbial degradation of fresh food at the postharvest stage without using synthetic fungicides, which are dangerous for the environment and people’s health. A good substitute for synthetic fungicides among them is the use of natural plant compounds, such as essential oils included or not included in the edible coatings. This review’s aim was to collect information from the scientific literature on the biological activity of essential oil, with or without edible coatings, against pathogens that cause the postharvest spoilage of many fruit belonging to Rosaceae family in order to develop appropriate substitute tactics for synthetic fungicides in the treatment of postharvest fruit diseases. Advances and obstacles surrounding emerging methods that may be useful for enhancing the effectiveness and dependability of essential oils were evaluated.
Does Carrier Envelope Phase Affect the Ionization Site in a Neutral Diatomic Molecule?
Authors: Schimmoller Alex , Pasquinilli Harrison , Landsman Alexandra S.
Published: April 2023

A recent work shows how to extract the ionization site of a neutral diatomic molecule by comparing Quantum Trajectory Monte Carlo (QTMC) simulations with experimental measurements of the final electron momenta distribution. This method was applied to an experiment using a 40-femtosecond infrared pulse, finding that a downfield atom is roughly twice as likely to be ionized as an upfield atom in a neutral nitrogen molecule. However, an open question remains as to whether an assumption of the zero carrier envelope phase (CEP) used in the above work is still valid for short, few-cycle pulses where the CEP can play a large role. Given experimentalists’ limited control over the CEP and its dramatic effect on electron momenta after ionization, it is desirable to see what influence the CEP may have in determining the ionization site. In this paper, we employ QTMC techniques to simulate strong-field ionization and electron propagation from neutral N2 using an intense 6-cycle laser pulse with various CEP values. Comparing simulated electron momenta to experimental data indicates that the ratio of down-to-upfield ions remains roughly 2:1 regardless of the CEP. This confirms that the ionization site of a neutral molecule is determined predominantly by the laser frequency and intensity, as well as the ground-state molecular wavefunction, and is largely independent of the CEP.
Intraluminal Therapy for Helicobacter pylori Infection—Comparison of Medicament Containing Tetracycline, Metronidazole, and Bismuth versus Amoxicillin, Metronidazole, and Clarithromycin: A Randomized Controlled Study
Authors: Liu Ting-Wen , Chen Yen-Po , Ho Cheng-Yu , Chen Ming-Jen , Wang Horng-Yuan , Shih Shou-Chuan , Liou Tai-Cherng
Published: April 2023

Helicobacter pylori (H. pylori) can be eradicated immediately via local application of single-dose medicament on endoscopic examination. In our previous report, “the eradication rate of intraluminal therapy for H. pylori infection (ILTHPI) is 53.7% (51/95) using medicament containing amoxicillin, metronidazole, and clarithromycin”. We aimed to evaluate the efficacy and adverse events of medicament containing tetracycline, metronidazole, and bismuth and to improve the efficacy of stomach acid control before ILTHPI. After usage of dexlansoprazole (60 mg b.i.d.) or vonoprazan (20 mg q.d.) for 3 days before ILTHPI, 103 of 104 (99.1%) symptomatic H. pylori-infected treatment-naïve patients achieved levels of stomach pH ≥ 6. Patients were randomized to receive ILTHPI with medicaments containing tetracycline, metronidazole, and bismuth (Group A, n = 52) or amoxicillin, metronidazole, and clarithromycin (Group B, n = 52). The eradication rate of ILTHPI was similar between Group A (76.5%; 39/51) and Group B (84.6%, 44/52) (p = 0.427) and the adverse event was mild diarrhea (2.9%; 3/104). The eradication rate significantly increased from 53.7% (51/95) to 84.6% (44/52) after acid control (p = 0.0004) for Group B patients. The overall eradication rates of successful ILTHPI plus 7-day non-bismuth (Group A) or 7-day bismuth (Group B) oral quadruple therapy for ILTHPI failure patients were both excellent (96.1% for Group A and 98.1% for Group B).
Multiple Actions of Telomerase Reverse Transcriptase in Cell Death Regulation
Authors: Palamarchuk Anastasia I. , Kovalenko Elena I. , Streltsova Maria A.
Published: April 2023

Telomerase reverse transcriptase (TERT), a core part of telomerase, has been known for a long time only for its telomere lengthening function by reverse transcription of RNA template. Currently, TERT is considered as an intriguing link between multiple signaling pathways. The diverse intracellular localization of TERT corresponds to a wide range of functional activities. In addition to the canonical function of protecting chromosome ends, TERT by itself or as a part of the telomerase complex participates in cell stress responses, gene regulation and mitochondria functioning. Upregulation of TERT expression and increased telomerase activity in cancer and somatic cells relate to improved survival and persistence of such cells. In this review, we summarize the data for a comprehensive understanding of the role of TERT in cell death regulation, with a focus on the interaction of TERT with signaling pathways involved in cell survival and stress response.
Carbon Nanomaterials-Based Screen-Printed Electrodes for Sensing Applications
Authors: Silva Rafael Matias , da Silva Alexsandra Dias , Camargo Jéssica Rocha , de Castro Bruna Santos , Meireles Laís Muniz , Silva Patrícia Soares , Janegitz Bruno Campos , Silva Tiago Almeida
Published: April 2023

Electrochemical sensors consisting of screen-printed electrodes (SPEs) are recurrent devices in the recent literature for applications in different fields of interest and contribute to the expanding electroanalytical chemistry field. This is due to inherent characteristics that can be better (or only) achieved with the use of SPEs, including miniaturization, cost reduction, lower sample consumption, compatibility with portable equipment, and disposability. SPEs are also quite versatile; they can be manufactured using different formulations of conductive inks and substrates, and are of varied designs. Naturally, the analytical performance of SPEs is directly affected by the quality of the material used for printing and modifying the electrodes. In this sense, the most varied carbon nanomaterials have been explored for the preparation and modification of SPEs, providing devices with an enhanced electrochemical response and greater sensitivity, in addition to functionalized surfaces that can immobilize biological agents for the manufacture of biosensors. Considering the relevance and timeliness of the topic, this review aimed to provide an overview of the current scenario of the use of carbonaceous nanomaterials in the context of making electrochemical SPE sensors, from which different approaches will be presented, exploring materials traditionally investigated in electrochemistry, such as graphene, carbon nanotubes, carbon black, and those more recently investigated for this (carbon quantum dots, graphitic carbon nitride, and biochar). Perspectives on the use and expansion of these devices are also considered.
In Vivo Biomarker Imaging: Paving the Way for Precision Medicine
Authors: Liu Guanshu , Yang Xing , Zhou Xin
Published: April 2023

In vivo bioimaging has become an indispensable tool in contemporary biomedical research and medicine [...]
Clinical Evaluation of Sleep Disorders in Parkinson’s Disease
Authors: Lauretani Fulvio , Testa Crescenzo , Salvi Marco , Zucchini Irene , Giallauria Francesco , Maggio Marcello
Published: April 2023

The paradigm of the framing of Parkinson’s disease (PD) has undergone significant revision in recent years, making this neurodegenerative disease a multi-behavioral disorder rather than a purely motor disease. PD affects not only the “classic” substantia nigra at the subthalamic nuclei level but also the nerve nuclei, which are responsible for sleep regulation. Sleep disturbances are the clinical manifestations of Parkinson’s disease that most negatively affect the quality of life of patients and their caregivers. First-choice treatments for Parkinson’s disease determine amazing effects on improving motor functions. However, it is still little known whether they can affect the quantity and quality of sleep in these patients. In this perspective article, we will analyze the treatments available for this specific clinical setting, hypothesizing a therapeutic approach in relation to neurodegenerative disease state.
Modality-Attention Promotes the Neural Effects of Precise Timing Prediction in Early Sensory Processing
Authors: Meng Jiayuan , Li Xiaoyu , Zhao Yingru , Li Rong , Xu Minpeng , Ming Dong
Published: April 2023

Precise timing prediction (TP) enables the brain to accurately predict the occurrence of upcoming events in millisecond timescale, which is fundamental for adaptive behaviors. The neural effect of the TP within a single sensory modality has been widely studied. However, less is known about how precise TP works when the brain is concurrently faced with multimodality sensory inputs. Modality attention (MA) is a crucial cognitive function for dealing with the overwhelming information induced by multimodality sensory inputs. Therefore, it is necessary to investigate whether and how the MA influences the neural effects of the precise TP. This study designed a visual–auditory temporal discrimination task, in which the MA was allocated to visual or auditory modality, and the TP was manipulated into no timing prediction (NTP), matched timing prediction (MTP), and violated timing prediction (VTP) conditions. Behavioral and electroencephalogram (EEG) data were recorded from 27 subjects, event-related potentials (ERP), time–frequency distributions of inter-trial coherence (ITC), and event-related spectral perturbation (ERSP) were analyzed. In the visual modality, precise TP led to N1 amplitude and 200–400 ms theta ITC variations. Such variations only emerged when the MA was attended. In auditory modality, the MTP had the largest P2 amplitude and delta ITC than other TP conditions when the MA was attended, whereas the distinctions disappeared when the MA was unattended. The results suggest that the MA promoted the neural effects of the precise TP in early sensory processing, which provides more neural evidence for better understanding the interactions between the TP and MA.
Exploring the Mediating Role of Executive Function in the Relationship between Aerobic Fitness and Academic Achievement in Adolescents
Authors: Park Seyun , Chun Haeyong , Etnier Jennifer L. , Yun Daehyun
Published: April 2023

(1) Background: The performance of physical activity at a sufficient volume can result in improvements in fitness. Aerobic fitness is a particular aspect of fitness that has consistently been shown to be related to both cognitive performance and academic achievement. Cognitive performance, particularly executive function, is itself predictive of academic achievement. It has been hypothesized that the benefits of aerobic fitness for academic achievement are due to improvements in executive function. This study explores the mediating role of cognitive performance on the relationship between physical fitness and academic achievement in high-school-aged adolescents. (2) Methods: High school students (N = 283, 127 male, 156 females, mean age = 16.05 years, SD = 0.41) performed a shuttle run test to assess aerobic fitness and completed the Stroop Color, Stroop Word, and Stroop Color–Word tests to measure information processing and inhibition. They also completed the National Union Academic Achievement Assessment (NUAAA) as part of their high school requirements. (3) Results: Mediation analyses showed that inhibition (performance in the Stroop Color–Word test) fully mediated the relationship between aerobic fitness and both mathematics and Korean performance. (4) Conclusions: This cross-sectional investigation suggests an important mediating role of cognitive performance related to executive function in understanding the relationship between aerobic fitness and the academic achievement of high-school-aged adolescents. This suggests that enhancements in performance related to executive function, which are attributed to increases in aerobic fitness, could explain the observed benefits for academic attainment.
Inflammation Control and Tumor Growth Inhibition of Ovarian Cancer by Targeting Adhesion Molecules of E-Selectin
Authors: Yang Bowen , Yin Shanmei , Zhou Zishuo , Huang Luyao , Xi Mingrong
Published: April 2023

Objective: The aim is to use E-selectin-binding peptide (ESBP) to actively recognize E-selectin, so allowing a drug delivery system to actively recognize the cells and inhibit the tumor growth of ovarian cancer by targeting adhesion molecules of E-selectin. An ovarian-cancer-directed drug delivery system was designed based on the high affinity of E-selectin-binding peptide (ESBP) to E-selectin. The effects and mechanisms of ESBP-bovine serum albumin (BSA) polymerized nanoparticles were investigated. Methods: BSA polymerized nanoparticles (BSANPs) and ESBP-BSANPs-paclitaxel (PTX) were prepared and their characteristics were measured. The in vitro targetability and cytotoxicity of ESBP-BSANPs-PTX were evaluated through in vitro drug uptake and MTT experiments. The mechanisms of ESBP-BSANPs-PTX were investigated via apoptosis, wound healing and immunohistochemistry assays. The in vivo targeting properties and drug effects were observed in a mouse tumor-bearing model. Results: In vitro experiments revealed an increase in the uptake of ESBP-BSANPs-FITC. The cytotoxicity of ESBP-BSANPs-PTX in A2780/CP70, HUVEC, RAW264.7 and ID8 cells was higher than that of PTX alone. ESBP-BSANPs-PTX increased cell apoptosis in a dose-dependent manner and exhibited a greater ability to inhibit cell migration than BSANPs-PTX. In vivo experiments demonstrated the targetability and good effects of ESBP-BSANPs. Conclusions: ESBP-BSANPs-PTX improve PTX targetability, provide tumor-specific and potent therapeutic activities, and show promise for the development of agents in preclinical epithelial ovarian cancer.
Peer Verbal Encouragement Enhances Offensive Performance Indicators in Handball Small-Sided Games
Authors: Sahli Faten , Sahli Hajer , Trabelsi Omar , Jebabli Nidhal , Zghibi Makram , Haddad Monoem
Published: April 2023

Objective: This study aimed at assessing the effects of two verbal encouragement modalities on the different offensive and defensive performance indicators in handball small-sided games practiced in physical education settings. Methods: A total of 14 untrained secondary school male students, aged 17 to 18, took part in a three-session practical intervention. Students were divided into two teams of seven players (four field players, a goalkeeper, and two substitutes). During each experimental session, each team played one 8 min period under teacher verbal encouragement (TeacherEN) and another under peer verbal encouragement (PeerEN). All sessions were videotaped for later analysis using a specific grid focusing on the balls played, balls won, balls lost, shots on goal, goals scored, as well as the ball conservation index (BCI), and the defensive efficiency index (DEI). Results: The findings showed no significant differences in favor of TeacherEN in all the performance indicators that were measured, whereas significant differences in favor of PeerEN were observed in balls played and shots on goal. Conclusions: When implemented in handball small-sided games, peer verbal encouragement can produce greater positive effects than teacher verbal encouragement in terms of offensive performance.
Timing of Ketogenic Dietary Therapy (KDT) Introduction and Its Impact on Cognitive Profiles in Children with Glut1-DS—A Preliminary Study
Authors: Barthold Martina , Jurkutat Anne , Goetz Regina , Schubring Lucia , Spiegler Juliane , Fries Ann-Sophie , Kiesel Lucia , Klepper Joerg
Published: April 2023

The aim of this research was to characterize cognitive abilities in patients with Glut1-Deficiency syndrome (Glut1DS) following ketogenic diet therapy (KDT). Methods: The cognitive profiles of eight children were assessed using the Wechsler Intelligence Scale (WISC-IV). The effect of ketogenic diet therapy (KDT) on individual subareas of intelligence was analyzed considering the potential influence of speech motor impairments. Results: Patients with Glut1DS showed a wide range of cognitive performance levels. Some participants showed statistically and clinically significant discrepancies between individual subdomains of intelligence. Both variables, KDT initiation as well as duration, had a positive effect on the overall IQ score. Significant correlations were partially found between the time of KDT initiation and the level of IQ scores, depending on the presence of expressive language test demands of the respective subtests of the WISC-IV. Accordingly, the participants benefited les in the linguistic cognitive domain. The discrepancies in cognitive performance profiles of patients with Glut1DS can be attributed to the possibility of a negative distortion of the results due to the influence of speech motor impairments. Conclusions: The individual access skills of test persons should be more strongly considered in test procedures for the assessment of intelligence to reduce the negative influence of motor deficits on test performance. Specific characterization and systematization of the speech disorder are indispensable for determining the severity of speech motor impairment in Glut1DS. Therefore, a stronger focus on dysarthria during diagnosis and therapy is necessary.
Prevalence and Predictive Factors for Exclusive Breastfeeding at Six Months among Thai Adolescent Mothers
Authors: Thaithae Suparp , Yimyam Susanha , Polprasarn Pridsadaporn
Published: April 2023

Exclusive breastfeeding (EBF) in the first six months of life is the best and the most frequent choice for infants since it has important benefits for the infants and their mothers. However, the exclusive breastfeeding rate in Thailand remains low, especially among adolescent mothers. This predictive correlation study aimed to investigate factors predicting breastfeeding at six months among 253 Thai adolescent mothers from nine hospitals of the Bangkok Metropolitan Administration. Data were collected by using seven questionnaires: the Personal Characteristics, Pregnancy Intention and Breastfeeding Practice, Perceived Benefits of Breastfeeding, Perceived Barriers to Breastfeeding, Breastfeeding Self-Efficacy, Family Support, Maternity Care Practice, and Digital Technology Literacy Questionnaires. Data were analyzed by using descriptive statistics and logistic regression. The findings revealed that the prevalence of EBF at six months among Thai adolescent mothers was only 17.39%, and predictive factors were occupation (work/study) (p = 0.034), digital technology literacy (p < 0.001), family support (p = 0.021), pregnancy intention (p = 0.001), breastfeeding self-efficacy (p = 0.016), and perceived benefits of breastfeeding (p = 0.004). These factors could, together, predict the EBF rate at six months among Thai adolescent mothers in 42.2% (Nagelkerke R2 = 0.422) of the cases. These findings may help health professionals to develop activities and strategies for promoting exclusive breastfeeding by increasing breastfeeding self-efficacy, perceived benefits of breastfeeding, and family support, as well as improving digital technology skill among Thai adolescent mothers, especially student/employed adolescents who have unintended pregnancies.
Factors for Prolonged Pain and Restriction of Movement Following Hemiepiphysiodesis Plating for the Correction of Lower Limb Malalignment in the Frontal Plane: An Explorative Analysis
Authors: Braun Sebastian , Brenneis Marco , Meurer Andrea , Holder Jana , Stief Felix
Published: April 2023

The correction of valgus leg malalignment in children using implant-mediated growth guidance is widely used and effective. Despite the minimal invasive character of the procedure, a relevant number of patients sustain prolonged pain and limited mobility after temporary hemiepiphysiodesis. Our aim was to investigate implant-associated risk factors (such as implant position and screw angulation), surgical- or anesthesia-related risk factors (such as type of anesthesia, use, and duration), and pressure of tourniquet or duration of surgery for these complications. Thirty-four skeletally immature patients with idiopathic valgus deformities undergoing hemiepiphysiodesis plating from October 2018–July 2022 were enrolled in this retrospective study. Participants were divided into groups with and without prolonged complications (persistent pain, limited mobility of the operated knee between five weeks and six months) after surgery. Twenty-two patients (65%) had no notable complications, while twelve patients (35%) had prolonged complications. Both groups differed significantly in plate position relative to physis (p = 0.049). In addition, both groups showed significant differences in the distribution of implant location (p = 0.016). Group 1 had a shorter duration of surgery than group 2 (32 min vs. 38 min, p = 0.032) and a lower tourniquet pressure (250 mmHg vs. 270 mmHg, p = 0.019). In conclusion, simultaneous plate implantation at the femur and tibia and metaphyseal plate positioning resulted in prolonged pain and a delay of function. In addition, the amplitude of tourniquet pressure or duration of surgery could play a factor.
Effect of Nigella sativa on Selected Gastrointestinal Diseases
Authors: Jarmakiewicz-Czaja Sara , Zielińska Magdalena , Helma Kacper , Sokal Aneta , Filip Rafał
Published: April 2023

Nigella sativa L. (family Ranunculaceae), also known as black cumin, has been used in cuisine around the world for many years. Due to its health-promoting properties, it can be used not only in the food industry but also in medicine. The main bioactive compound contained in the black cumin extract is thymoquinone (TQ), which has a special therapeutic role. The results of research in recent years confirmed its hypoglycemic, hypolipemic, and hepatoprotective effects, among others. In addition, the results of laboratory tests also indicate its immunomodulatory and anticancer effects, although there is still a lack of data on the mechanisms of how they are involved in the fight against cancer. Including this plant material in one’s diet can be both an element of prophylaxis and therapy supporting the treatment process, including pharmacological treatment. However, attention should be paid to its potential interactions with drugs used in the treatment of chronic diseases.
Plasma-Assisted Nitrogen Doping of Langmuir–Blodgett Self-Assembled Graphene Films
Authors: Tomašević-Ilić Tijana , Škoro Nikola , Jovanović Đorđe , Puač Nevena , Spasenović Marko
Published: April 2023

Graphene films prepared from solution and deposited by Langmuir–Blodgett self-assembly technique (LBSA) were treated with radio-frequency (13.56 MHz) nitrogen plasma in order to investigate the influence of the time of nitrogen plasma exposure on the work function, sheet resistance, and surface morphology of LBSA graphene films. Kelvin probe force microscopy and sheet resistance measurements confirm nitrogen functionalization of our films, with the Fermi level shifting in a direction that indicates binding to a pyridinic and/or pyrrolic site. Upon 1 min of nitrogen plasma exposure, the sheet resistance decreases and there is no obvious difference in film morphology. However, plasma exposure longer than 5 min leads to the removal of graphene flakes and degradation of graphene films, in turn, affecting the flake connectivity and increasing film resistance. We show that by changing the exposure time, we can control the work function and decrease sheet resistance, without affecting surface morphology. Controllability of the plasma technique has an advantage for graphene functionalization over conventional doping techniques such as chemical drop-casting. It allows for the controllable tuning of the work function, surface morphology, and sheet resistance of LBSA graphene films, which is substantial for applications in various optoelectronic devices.
A Novel FPGA Implementation of the NAND-PUF with Minimal Resource Usage and High Reliability
Authors: Della Sala Riccardo , Scotti Giuseppe
Published: April 2023

In this work we propose a novel implementation on recent Xilinx FPGA platforms of a PUF architecture based on the NAND SR-latch (referred to as NAND-PUF in the following) which achieves an extremely low resource usage with very good overall performance. More specifically, a 4 bit NAND-PUF macro has been designed referring to the Artix-7 platform occupying only 2 slices. The optimum excitation sequence has been determined by analysing the reliability versus the excitation time of the PUF cells under supply voltage variations. A 128 bit NAND-PUF has been tested on 16 FPGA boards under supply voltage and temperature variations and measured performances have been compared against state-of-the-art PUFs from the literature. The comparison has shown that the proposed PUF implementation exhibits the best reliability performance while occupying the minimum FPGA resource usage achieved in the PUF literature.
Relative Stability of Pyrazinamide Polymorphs Revisited: A Computational Study of Bending and Brittle Forms Phase Transitions in a Broad Temperature Range
Authors: Dubok Aleksandr S. , Rychkov Denis A.
Published: April 2023

Pyrazinamide may exist in at least four known polymorphic forms, which were obtained experimentally. One of these polymorphs, (α), shows outstanding mechanical properties, demonstrating a significant anisotropic plasticity in a three-point bending test, while the δ form was brittle. Despite a δ → α transition as well as β and γ behavior being experimentally studied, the relative stability of pyrazinamide polymorphs remains unclear and even controversial. In this work we provide a pure computational study of the thermodynamic relationships between all four polymorphs as a function of temperature using periodic DFT calculations. It was shown that the β but not the δ form is the most stable at low temperatures. Moreover, the relative stability of the δ form in comparison to α is questioned, showing that the “brittle to bending” δ → α transition was kinetically hindered in the experiments. We show that α and γ polymorphs were stabilized at higher temperatures due to an entropy term. Finally, the calculated stability of the bending α form of pyrazinamide at room temperature was in perfect agreement with previous experiments, which showed a transformation of all other forms to α during six month storage or grinding.
Modeling COVID-19 Infection Rates by Regime-Switching Unobserved Components Models
Authors: Haimerl Paul , Hartl Tobias
Published: April 2023

The COVID-19 pandemic is characterized by a recurring sequence of peaks and troughs. This article proposes a regime-switching unobserved components (UC) approach to model the trend of COVID-19 infections as a function of this ebb and flow pattern. Estimated regime probabilities indicate the prevalence of either an infection up- or down-turning regime for every day of the observational period. This method provides an intuitive real-time analysis of the state of the pandemic as well as a tool for identifying structural changes ex post. We find that when applied to U.S. data, the model closely tracks regime changes caused by viral mutations, policy interventions, and public behavior.
Hybrid General Regression NN Model for Efficient Operation of Centralized TEG System under Non-Uniform Thermal Gradients
Authors: Khan Noman Mujeeb , Ahmed Abbas , Haider Syed Kamran , Zafar Muhammad Hamza , Mansoor Majad , Akhtar Naureen
Published: April 2023

The global energy demand, along with the proportionate share of renewable energy, is increasing rapidly. Renewables such as thermoelectric generators (TEG) systems have lower power ratings but a highly durable and cost-effective renewable energy technology that can deal with waste heat energy. The main issues associated with TEG systems are related to their vigorous operating conditions. The dynamic temperature gradient across TEG surfaces induces non-uniform temperature distribution (NUTD) that significantly impacts the available output electrical energy. The mismatching current impact may lower the energy yield by up to 70% of extractable thermal energy. As a solution, a hybrid general regression neural network (GRNN) orca predation algorithm (OPA) is proposed; backpropagation limitations are minimized by utilizing the stochastic optimization algorithm named OPA. The conclusions are evaluated and contrasted with highly improved versions of the conventional particle swarm optimization (PSO), grey wolf optimizer (GWO), and Harris hawk optimization (HHO). A detailed analytical and statistical analysis is carried out through five distinct case studies, including field stochastic data study, NUTD, varying temperature, and load studies. Along with statistical matrix errors such as MAE, RMSE, and RE, the results are assessed in terms of efficiency, tracking, and settling time. The results show that superior performance is achieved by the proposed GRNN-OPA based MPPT by 35% faster tracking, and up to 90–110% quicker settling time which, in turn, enables the 4–8% higher energy accumulation over a longer period of operation. A low-cost experimental setup is devised to further validate the practicality of the proposed techniques. From such comprehensive analysis, it can be safely concluded that the proposed GRNN-OPA successfully undertakes the drawbacks of existing classical MPPT methods with higher efficiency.
Renewable Energy and Governance Resilience in the Gulf
Authors: Sim Li-Chen
Published: April 2023

The six Gulf monarchies—Bahrain, Kuwait, Oman, Qatar, Saudi Arabia, and United Arab Emirates (UAE)—are more politically stable than their peers in the Middle East and North Africa. Explanations for governance resilience range from repression to neopatrimonial and instrumental legitimacy, hydrocarbon-based rentierism, and permissive regional and international environments. This paper considers, in view of the proliferation and uptake of renewable energy in the Gulf, how governance resilience may be affected as a result of changes in state-society relations during the energy transition away from a fossil-fuel-based energy system. It offers a qualitative analysis of the impact of renewable energy deployment in the Gulf, supported by a rich array of secondary literature and data. It also offers a deep, if brief, dive to highlight intra-regional nuances. The authors conclude that in the short term, renewable energy deployment has a very modest impact given its limited share of power generation. In the longer term, even assuming that stated ambitions for renewable energy are fulfilled, no negative impact on monarchial resilience is expected thanks to gains in legitimacy and revenue streams, as well as purposeful alignment with an external environment supportive of renewable power in developing countries.
The Lithium-Ion Battery Recycling Process from a Circular Economy Perspective—A Review and Future Directions
Authors: Sheth Rahil Parag , Ranawat Narendra Singh , Chakraborty Ayon , Mishra Rajesh Prasad , Khandelwal Manoj
Published: April 2023

Ever since the introduction of lithium-ion batteries (LIBs) in the 1970s, their demand has increased exponentially with their applications in electric vehicles, smartphones, and energy storage systems. To cope with the increase in demand and the ensuing environmental effects of excessive mining activities and waste production, it becomes crucial to explore ways of manufacturing LIBs from the resources that have already been extracted from nature. It is possible by promoting the re-usage, refurbishing, and recycling of the batteries and their constituent components, rethinking the fundamental design of devices using these batteries, and introducing the circular economy model in the battery industry. This paper through a literature review provides the current state of CE adoption in the lithium-ion battery industry. The review suggests that the focus is mostly on recycling at this moment in the battery industry, and a further understanding of the process is needed to better adapt to other CE practices such as reuse, remanufacture, refurbishment, etc. The paper also provides the steps involved in the recycling process and, through secondary case studies, shows how some of the industries are currently approaching battery recycling. Thus, this paper, through review and secondary cases, helps us to understand the current state of LIB recycling and CE adoption.
Sustainable Co-Management of Acid Mine Drainage with Struvite Synthesis Effluent: Pragmatic Synergies in Circular Economy
Authors: Masindi Vhahangwele , Mbhele Ryneth , Foteinis Spyros
Published: April 2023

Herein, the alkaline supernatant of a struvite recovery system from municipal wastewater was successfully co-managed with acid mine drainage (AMD). Various ratios (v/v) of AMD to struvite supernatant were examined, and the quality of the passively co-treated effluent and of the generated sludge were examined using state-of-the-art analytical techniques including ICP-OES, FE-SEM/FIB/EDX, XRD, XRF, and FTIR. The optimum ratio was 1:9, where metals and sulphate were largely removed from AMD, i.e., from higher to lower score Fe (~100%) ≥ Pb (~100%) ≥ Ni (99.6%) ≥ Cu (96%) ≥ As (95%) ≥ Al (93.7%) ≥ Zn (92.7%) > Ca (90.5%) > Mn (90%) ≥ Cr (90%) > sulphate (88%) > Mg (85.7%), thus implying that opportunities for mineral recovery could be pursued. The pH of the final effluent was regulated to acceptable discharge levels, i.e., 6.5 instead of 2.2 (AMD) and 10.5 (struvite supernatant), while a notable reduction in the electrical conductivity further implied the attenuation of contaminants. Overall, results suggest the feasibility of the passive co-treatment of these wastewater matrices and that opportunities for direct scaling up exist (e.g., using waste stabilization ponds). Furthermore, apart from the initial recovery of struvite from municipal wastewater, metals could also be recovered from AMD and water could be reclaimed, therefore introducing circular economy and zero liquid discharge in wastewater treatment and management.
Organic Materials and AMF Addition Promote Growth of Taxodium ‘zhongshanshan’ by Improving Soil Structure
Authors: Zeng Jingyi , Ma Shilin , Liu Jing , Qin Shenghua , Liu Xin , Li Tao , Liao Yi , Shi Yuxuan , Zhang Jinchi
Published: April 2023

Soil salinization is considered a type of global-scale soil degradation, whereby excessive salinity severely diminishes soil health, which is primarily manifested through disrupted soil structures and reduced fertility. Furthermore, plant growth capacity is inhibited, and productivity is diminished. Consequently, the improvement of saline soils is regarded as a particularly important aspect of enhancing land production. To elucidate the roles of organic amendments and mycorrhizal fungi in the improvement of saline soils, seven treatments were set up, including biochar alone (B), straw alone (S), arbuscular mycorrhizal fungi alone (A), biochar in combination with arbuscular mycorrhizal fungi (BA), straw in combination with arbuscular mycorrhizal fungi (SA), and a control (CK). The results revealed that the seedling height growth rate under the BA treatment was significantly higher than that of the CK by 31.66%. The capillary porosity of the soil under the addition of BA was significantly higher than the CK by 3.17% in the 0–20 cm soil layer. The BA treatment reduced the fractal dimension of soil aggregates considerably by 19.06% in the 0–20 cm soil layer, and 13.71% in the 40–60 cm soil layer in contrast to the CK, respectively. In addition, the positive effects of the BA treatment were significant in the 20–40 cm soil layer. Overall, the application of biochar alone promoted the water stability of soil aggregates. The combination of arbuscular mycorrhizal fungi and biochar promoted plant growth, improved soil pore structures, promoted agglomerate water stability, and led to improved microbial activities. The results showed that organic amendments applied in conjunction with AMF improved the environment of salinized soil, which is a key factor in the promotion of plant growth and the long-term stability of soil health. This study provides a key technical basis for remediation of salinized soil.
Research on the Resilience Assessment of Rural Landscapes in the Context of Karst Rocky Desertification Control: A Case Study of Fanhua Village in Guizhou Province
Authors: Ying Bin , Li Sensen , Xiong Kangning , Hou Yufeng , Liu Ting , Sun Ruonan
Published: April 2023

The ecological rehabilitation project has greatly curbed the serious problem of karst rocky desertification (KRD) in southern China and significantly changed the ecological environment and landscape pattern of the karst rocky desertification control areas (KRDCA). As one of the most important social–ecological fragile areas in the world, rural landscapes in KRDCA still show a strong sensitivity to disturbance. To reduce risks and improve the resilience of landscapes, this paper constructs a framework for assessing rural landscape resilience in KRDCA from the three dimensions of ecology, engineering, and social culture, based on the concept of resilience defined by the United Nations International Agency for Disaster Reduction. Considering the characteristics of rural landscapes in KRDCA, we select typical villages for empirical study. The results show the following: (1) The KRDCA is highly sensitive to natural disasters due to its special dual geomorphic structure characteristics. The disaster preparedness capacity of villages is the key factor determining the resilience of rural landscapes. The analysis of the disaster preparedness capacities of rural landscape structures with different vulnerability characteristics can be used as an effective means of evaluating the resilience level of rural landscapes in KRDCA. (2) Based on the empirical analysis of Fanhua village, which is a typical KRDCA in southern China, we found that the ecological system and engineering system of the village landscape have high resilience, while the resilience of the social and cultural systems are weak. This is due to the fact that the large number of rural population emigration in recent years has resulted in villages being at the key node of the reorganization of the social and cultural value system. The unstable sociocultural value system reduces the ability of rural landscapes to adapt to disturbance or environmental change. The study results could guide improvement strategies for subsequent landscape planning and inspire new ideas and methods for the implementation of rural revitalization strategies and the improvement of landscape resilience in KRDCA.
Influence of Weathering on the Structural Performance of Sheathing-to-Timber Connections
Authors: Cowled Craig J. L. , Slattery Tom P. , Crews Keith , Brooke Harrison
Published: April 2023

The sheathing-to-timber connection (STC) is a critical component of timber-framed shear walls. The STC provides the shear wall system with its racking resistance, while anchors and tiedowns provide resistance to sliding and overturning, respectively. Because building materials are exposed to weathering during construction, this study aims to quantify the influence of weathering on the structural performance of STCs. To achieve this aim, a total of 117 small-scale specimens were fabricated with 5 different sheathing types and 2 different timber species. Each specimen comprised 2 panels of sheathing connected to 2 short lengths of pine timber (90×35 mm cross-section), with a total of 16/2.8ϕ×30 mm (l) galvanised clouts at 45 mm spacings. Some specimens were tested under the EN 594 monotonic loading protocol and others were tested under the ISO 16670 cyclic loading protocol. Some specimens were exposed to the weather for a period of 6 months before being tested, while others were stored in an air-conditioned environment before being tested. The results show that weathering reduces the ultimate and yield capacity of STC connections by 3% and 5% on average, respectively; however, this result is not statistically significant for most sheathing types. The results varied, with some configurations having an ultimate capacity up to 16% higher and others having an ultimate capacity as much as 20% lower for weathered specimens compared to unweathered specimens. However, weathering reduces the stiffness of STCs by 61% and ductility by 50%, a statistically significant result. For most sheathing types, these findings do not support reductions to the design capacity of STCs that have been exposed to weathering.
Physicochemical, Microbiological, and Sensory Properties of Set-Type Yoghurt Supplemented with Camel Casein Hydrolysate
Authors: Metwalli Ali A. , Ismail Elsayed A. , Elkhadragy Manal F. , Yehia Hany M.
Published: April 2023

The microbiological, rheological, and sensory properties of set-type yoghurt were investigated in the presence of camel and cow casein hydrolysates produced by the action of trypsin enzymes. The hydrolysates significantly decreased the fermentation and coagulation time of the yoghurt production. The rate of pH decrease was significantly (p < 0.05) higher in samples treated with cow casein hydrolysate in comparison with control samples. Compared with the control, the cell growth of the yoghurt culture increased with the supplementation of the casein hydrolysate. Moreover, the survival of lactic acid bacteria (LAB) was enhanced by the addition of hydrolysates. The fortification of cow milk with camel and cow casein hydrolysates contributed to a significant improvement of the rheological and sensory properties of yoghurt. In conclusion, camel and cow casein hydrolysate could be used as a supplement in set-type yoghurt production with a potential beneficial effect on fermentation time, survival of total bacterial count, and overall acceptability.
Quantitative PCR Assay as a Tool for the Detection of Lactobacilli in Sicilian Table Olives Produced at an Industrial Scale
Authors: Vaccalluzzo Amanda , Pino Alessandra , Bosco Georgiana , Caggia Cinzia , Randazzo Cinzia Lucia
Published: April 2023

Table olives are an important fermented product of the Mediterranean area consumed all over the world. In our era, the food industry requires a safe and stable final product with desirable characteristics for the consumer. In the present study, two different experimental fermentations (L, with Lactiplantibacillus plantarum strains, and LY, with L. plantarum strains and Wickerhamomyces anomalus strain) were conducted and monitored up to 180 days and compared with a spontaneous fermentation, used as control (C). The safety and stability of table olives were determined by applying a plate count and quantitative real-time PCR (qPCR) approach. Compared with the control sample (C), experimental fermentations showed a faster acidification and a good inhibition rate of spoilage bacteria, indicating the safety of the process. Quantitative PCR data confirmed the abundance of the Lactobacillus group in both experimental table olives, confirming the importance of the starter cultures for the stability of the final product. In conclusion, the use of starter cultures ensures the safety of industrially produced table olives, and the application of qPCR seems to be a promising tool to detect and quantify lactobacilli as a positive biomarker of table olive fermentation.