bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurocognitive and cerebellar function in ADHD, autism and spinocerebellar ataxia
Authors: Cundari Maurizio , Vestberg Susanna , Gustafsson Peik , Gorcenco Sorina , Rasmussen Anders
Published: June 2023

The cerebellum plays a major role in balance, motor control and sensorimotor integration, but also in cognition, language, and emotional regulation. Several neuropsychiatric disorders such as attention deficit-hyperactivity disorder (ADHD), autism spectrum disorder (ASD), as well as neurological diseases such as spinocerebellar ataxia type 3 (SCA3) are associated with differences in cerebellar function. Morphological abnormalities in different cerebellar subregions produce distinct behavioral symptoms related to the functional disruption of specific cerebro-cerebellar circuits. The specific contribution of the cerebellum to typical development may therefore involve the optimization of the structure and function of cerebro-cerebellar circuits underlying skill acquisition in multiple domains. Here, we review cerebellar structural and functional differences between healthy and patients with ADHD, ASD, and SCA3, and explore how disruption of cerebellar networks affects the neurocognitive functions in these conditions. We discuss how cerebellar computations contribute to performance on cognitive and motor tasks and how cerebellar signals are interfaced with signals from other brain regions during normal and dysfunctional behavior. We conclude that the cerebellum plays a role in many cognitive functions. Still, more clinical studies with the support of neuroimaging are needed to clarify the cerebellum’s role in normal and dysfunctional behavior and cognitive functioning.
Refueling the post COVID-19 brain: potential role of ketogenic medium chain triglyceride supplementation: an hypothesis
Authors: Juby Angela G. , Cunnane Stephen C. , Mager Diana R.
Published: June 2023

COVID-19 infection causes cognitive changes in the acute phase, but also after apparent recovery. Over fifty post (long)-COVID symptoms are described, including cognitive dysfunction (“brain fog”) precluding return to pre-COVID level of function, with rates twice as high in females. Additionally, the predominant demographic affected by these symptoms is younger and still in the workforce. Lack of ability to work, even for six months, has significant socio-economic consequences. This cognitive dysfunction is associated with impaired cerebral glucose metabolism, assessed using 18F-fluorodeoxyglucose-positron emission tomography (FDG-PET), showing brain regions that are abnormal compared to age and sex matched controls. In other cognitive conditions such as Alzheimer’s disease (AD), typical patterns of cerebral glucose hypometabolism, frontal hypometabolism and cerebellar hypermetabolism are common. Similar FDG-PET changes have also been observed in post-COVID-19, raising the possibility of a similar etiology. Ketone bodies (B-hydroxybutyrate, acetoacetate and acetone) are produced endogenously with very low carbohydrate intake or fasting. They improve brain energy metabolism in the face of cerebral glucose hypometabolism in other conditions [mild cognitive impairment (MCI) and AD]. Long-term low carbohydrate intake or prolonged fasting is not usually feasible. Medium chain triglyceride (MCT) is an exogenous route to nutritional ketosis. Research has supported their efficacy in managing intractable seizures, and cognitive impairment in MCI and AD. We hypothesize that cerebral glucose hypometabolism associated with post COVID-19 infection can be mitigated with MCT supplementation, with the prediction that cognitive function would also improve. Although there is some suggestion that post COVID-19 cognitive symptoms may diminish over time, in many individuals this may take more than six months. If MCT supplementation is able to speed the cognitive recovery, this will impact importantly on quality of life. MCT is readily available and, compared to pharmaceutical interventions, is cost-effective. Research shows general tolerability with dose titration. MCT is a component of enteral and parenteral nutrition supplements, including in pediatrics, so has a long record of safety in vulnerable populations. It is not associated with weight gain or adverse changes in lipid profiles. This hypothesis serves to encourage the development of clinical trials evaluating the impact of MCT supplementation on the duration and severity of post COVID-19 cognitive symptoms.
Chronic pain, chronic stress and substance use: overlapping mechanisms and implications
Authors: Schaffer J. , Fogelman N. , Seo D. , Sinha R.
Published: June 2023

Chronic pain is among the most common reasons adults in the U.S. seek medical care. Despite chronic pain's substantial impact on individuals' physical, emotional, and financial wellness, the biologic underpinnings of chronic pain remain incompletely understood. Such deleterious impact on an individuals' wellness is also manifested in the substantial co-occurrence of chronic stress with chronic pain. However, whether chronic stress and adversity and related alcohol and substance misuse increases risk of developing chronic pain, and, if so, what the overlapping psychobiological processes are, is not well understood. Individuals suffering with chronic pain find alleviation through prescription opioids as well as non-prescribed cannabis, alcohol, and other drugs to control pain, and use of these substances have grown significantly. Substance misuse also increases experience of chronic stress. Thus, given the evidence showing a strong correlation between chronic stress and chronic pain, we aim to review and identify overlapping factors and processes. We first explore the predisposing factors and psychologic features common to both conditions. This is followed by examining the overlapping neural circuitry of pain and stress in order to trace a common pathophysiologic processes for the development of chronic pain and its link to substance use. Based on the previous literature and our own findings, we propose a critical role for ventromedial prefrontal cortex dysfunction, an overlapping brain area associated with the regulation of both pain and stress that is also affected by substance use, as key in the risk of developing chronic pain. Finally, we identify the need for future research in exploring the role of medial prefrontal circuits in chronic pain pathology. Critically, in order to alleviate the enormous burden of chronic pain without exacerbating the co-occurring substance misuse crisis, we emphasize the need to find better approaches to treat and prevent chronic pain.
Neuroimaging of pediatric tumors of the sellar region—A review in light of the 2021 WHO classification of tumors of the central nervous system
Authors: Maia Rúben , Miranda André , Geraldo Ana Filipa , Sampaio Luísa , Ramaglia Antonia , Tortora Domenico , Severino Mariasavina , Rossi Andrea
Published: June 2023

Sellar/suprasellar tumors comprise about 10% of all pediatric Central Nervous System (CNS) tumors and include a wide variety of entities, with different cellular origins and distinctive histological and radiological findings, demanding customized neuroimaging protocols for appropriate diagnosis and management. The 5th edition of the World Health Organization (WHO) classification of CNS tumors unprecedently incorporated both histologic and molecular alterations into a common diagnostic framework, with a great impact in tumor classification and grading. Based on the current understanding of the clinical, molecular, and morphological features of CNS neoplasms, there have been additions of new tumor types and modifications of existing ones in the latest WHO tumor classification. In the specific case of sellar/suprasellar tumors, changes include for example separation of adamantinomatous and papillary craniopharyngiomas, now classified as distinct tumor types. Nevertheless, although the current molecular landscape is the fundamental driving force to the new WHO CNS tumor classification, the imaging profile of sellar/suprasellar tumors remains largely unexplored, particularly in the pediatric population. In this review, we aim to provide an essential pathological update to better understand the way sellar/suprasellar tumors are currently classified, with a focus on the pediatric population. Furthermore, we intend to present the neuroimaging features that may assist in the differential diagnosis, surgical planning, adjuvant/neoadjuvant therapy, and follow-up of this group of tumors in children.
Custom-made semi-joint prosthesis replacement combined ligament advanced reinforcement system (LARS) ligament reconstruction for the limb salvage surgery of malignant tumors in the distal femur in skeletal immature children
Authors: Zan Pengfei , Shen Jiakang , Liu Kaiyuan , Wang Hongsheng , Cai Zhengdong , Ma Xiaojun , Sun Wei
Published: June 2023

Purpose

To explore the application of Custom-made Semi-joint prosthesis replacement combined with Ligament Advanced Reinforcement System (LARS) ligament reconstruction for the limb salvage surgery (LSS) of malignant tumors in the distal femur and provide selections for the LSS of malignant tumors in skeletal immature children.

Methods

A total of 8 children with malignant tumors in the distal femur who underwent Custom-made Semi-joint prosthesis replacement combined LARS ligament reconstruction for LSS from January, 2018 until December, 2019 in our bone and soft tissue tumor center were retrospectively recruited. The prosthesis related complications, oncological prognosis and knee function were observed, and the surgical efficacy was comprehensively evaluated.

Results

The average follow-up time was 36.6 months (30–50 months). The average osteotomy length was 13.2 cm (8–20 cm) according to the preoperative imaging results and the length of the customized prosthesis. Two years after operation, the average MSTS-93 score was 24.4 (16–29) which indicated good limb functions. The range of motion of the knee was 0°–120°, with an maximum average of 100°. At last follow-up, the average height of the children increased by 8.4 cm (6–13 cm), and the average limb shortening was 2.7 cm (1.8–4.6 cm). One patient had wound complications in the early postoperative period, wound scab fell off to form superficial ulcer, in whom debridement and suturing were performed. One patient developed hematogenous disseminated prosthesis infection 2 years after surgery, and the prosthesis is now in situ with anti-infection treatment. One patient developed pulmonary metastasis during follow-up, and received chemotherapy and targeted therapy with lesion well controlled. At the last follow-up, there was no local tumor recurrence or prosthesis loosening.

Conclusion

Under the premise of appropriate case selection, customized semi-joint prosthesis replacement combined with LARS ligament reconstruction provides a new option for LSS in children with distal femur malignant tumors. LARS ligament reconstruction ensures the stability and range of motion of the knee joint, which maximally preserves the epiphysis of the tibia side and the growth function of the tibia side, reduces the complications of limb length inequality in the long term and creates conditions for limb lengthening or total joint replacement in adults.
Minipuberty assessment in newborns with hypoxic ischemic encephalopathy treated with therapeutic hypothermia: a single-center case–control study
Authors: Lanciotti Lucia , Sica Rossella , Penta Laura , Parisi Francesca , Argentiero Alberto , Radicioni Maurizio , Di Cara Giuseppe , Di Genova Francesca , Verrotti Alberto , Troiani Stefania , Esposito Susanna Maria Roberta
Published: June 2023

Introduction

The aim of our single-center case–control study is to evaluate whether minipuberty occurs in patients with hypoxic ischemic encephalopathy (HIE) who underwent therapeutic hypothermia (TH). We intend to conduct this evaluation by confronting the values of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and the values of testosterone in males and estradiol in females between newborns with HIE and in subsequent TH and healthy controls.

Methods

We enrolled 40 patients (age: 56–179 days; 23 males), of whom 20 met the inclusion criteria for the case group and who underwent TH. A blood sample was taken from each patient at approximately 10 weeks of age to evaluate FSH and LH from the serum samples of all patients and to evaluate 17-beta estradiol (E2) and testosterone levels, respectively, from the serum samples of female and male patients.

Results

It was found that minipuberty occurred in the case group patients, with no significant differences reported from the control group and with hormonal serum levels comparable to healthy infants of the control group (FSH 4.14 mUI/ml ± 5.81 SD vs. 3.45 mUI/ml ± 3.48 SD; LH 1.41 mUI/ml ±1.29 SD vs. 2.04 mUI/ml ±1.76 SD; testosterone in males 0.79 ng/ml ± 0.43 SD vs. 0.56 ng/ml ± 0.43 SD; 17-beta estradiol in females 28.90 pg/ml ± 16.71 SD vs. 23.66 pg/ml ± 21.29 SD).

Discussion

The results of the present study may pave the way for further research and the evaluation of more possible advantages of TH.
NRICM101 ameliorates SARS-CoV-2–S1-induced pulmonary injury in K18-hACE2 mice model
Authors: Wei Wen-Chi , Tsai Keng-Chang , Liaw Chia-Ching , Chiou Chun-Tang , Tseng Yu-Hwei , Liao Geng-You , Lin Yu-Chi , Chiou Wen-Fei , Liou Kuo-Tong , Yu I-Shing , Shen Yuh-Chiang , Su Yi-Chang
Published: June 2023

The coronavirus disease 2019 (COVID-19) pandemic continues to represent a challenge for public health globally since transmission of different variants of the virus does not seem to be effectively affected by the current treatments and vaccines. During COVID-19 the outbreak in Taiwan, the patients with mild symptoms were improved after the treatment with NRICM101, a traditional Chinese medicine formula developed by our institute. Here, we investigated the effect and mechanism of action of NRICM101 on improval of COVID-19-induced pulmonary injury using S1 subunit of the SARS-CoV-2 spike protein-induced diffuse alveolar damage (DAD) of hACE2 transgenic mice. The S1 protein induced significant pulmonary injury with the hallmarks of DAD (strong exudation, interstitial and intra-alveolar edema, hyaline membranes, abnormal pneumocyte apoptosis, strong leukocyte infiltration, and cytokine production). NRICM101 effectively reduced all of these hallmarks. We then used next-generation sequencing assays to identify 193 genes that were differentially expressed in the S1+NRICM101 group. Of these, three (Ddit4, Ikbke, Tnfaip3) were significantly represented in the top 30 enriched downregulated gene ontology (GO) terms in the S1+NRICM101 group versus the S1+saline group. These terms included the innate immune response, pattern recognition receptor (PRR), and Toll-like receptor signaling pathways. We found that NRICM101 disrupted the interaction of the spike protein of various SARS-CoV-2 variants with the human ACE2 receptor. It also suppressed the expression of cytokines IL-1β, IL-6, TNF-α, MIP-1β, IP-10, and MIP-1α in alveolar macrophages activated by lipopolysaccharide. We conclude that NRICM101 effectively protects against SARS-CoV-2-S1-induced pulmonary injury via modulation of the innate immune response, pattern recognition receptor, and Toll-like receptor signaling pathways to ameliorate DAD.
Combination of deep learning with representation learning in X-ray prohibited item detection
Authors: Rao Jianghao , Qin Peng , Zhou Gaofan , Li Meihui , Zhang Jianlin , Bao Qiliang , Peng Zhenming
Published: June 2023

During X-ray inspection detection, a detector converts the collected X-rays from objects into electrical signals, which are then transmitted to a computer for image processing and analysis. From the aspect of digital image processing, detection tasks mainly focus on data processing and transformation to identify valuable features, which make the algorithms more effective. The consistent requirement for speed and accuracy in X-ray prohibited item detection is still not fully satisfied, especially in pictures obtained under special imaging conditions. For noisy X-ray images with heavy occlusion, a direct and suitable approach of representation learning is the optimal solution. According to our study, we realized that heterogeneous information fusion from different extraction approaches can be applied effectively to overcome this issue. We proposed two innovative algorithms to extract effective features of X-ray objects to significantly improve the efficiency of X-ray prohibited item detection. The brief model we proposed fuses the representations learned from the noisy X-ray images and outperforms the best model (DOAM-O) so far on OPIXray. Furthermore, the attention module we designed to select information on deep learning and representation strengthens the model; considering this, the model utilizes lesser time for both training and inference, which makes it easier to be trained on a lightweight computing device.
A comprehensive study of the aerodynamic characteristics of an aspect ratio of 6.25:1 inclined square prism
Authors: Chen Zengshun , Ning Guoliang , Zhang Likai , Li Sijia , Wang Siyu , Xu Yemeng , Yuan Chenfeng , Tse T. K. , Li Cruz Y. , Xue Xuanyi
Published: June 2023

Inclined prismatic structures have grown in popularity in civil engineering due to increased architectural and aesthetic demands, leading to a surging need to study their aerodynamic properties. In this study, an inclined square prism was examined using wind tunnel tests and numerical simulations with an aspect ratio of 6.25:1. The Synchronous Multi-Pressure Sensing System (SMPSS) wind tunnel technique was used to evaluate the aerodynamic characteristics of the structure, including surface pressure distribution, local force spectra, force coefficient, coherence, and the Strouhal number. Moreover, the numerical Large-eddies simulation (LES) were also deployed to investigate the flow field morphology around the structure. Results show that the orientation, direction, and angle of inclination direction significantly affect the prism’s aerodynamic characteristics. Several flow field phenomena were also revealed. Forward inclination enhances the downwash flow, whereas the backward counterpart enhances the upwash flow, and transverse inclination produces a noticeable modification in the vortex morphology near the prism base. These findings can inform structural design and contaminant dispersion efforts.
Hibiscus attenuates renovascular hypertension–induced aortic remodeling dose dependently: the oxidative stress role and Ang II/cyclophilin A/ERK1/2 signaling
Authors: ShamsEldeen Asmaa Mohammed , Fawzy Ahmed , Ashour Hend , Abdel-Rahman Marwa , Nasr Hend Elsayed , Mohammed Lina Abdelhady , Abdel Latif Noha Samir , Mahrous Amr M. , Abdelfattah Shereen
Published: June 2023

Introduction: The high levels of angiotensin II (Ang II) can modify the vascular tone, enhance vascular smooth muscle cells (VSMCs) proliferation and hypertrophy and increase the inflammatory cellular infiltration into the vessel wall. The old herbal nonpharmacological agent, Hibiscus (HS) sabdariffa L has multiple cardioprotective impacts; thus, we investigated the role of HS extract in amelioration of renovascular hypertension (RVH)-induced aortic remodeling.

Materials and methods: Thirty-five rats (7/group) were randomly allocated into 5 groups; group: I: Control-sham group, and RVH groups; II, III, IV, and V. The rats in RVH groups were subjected to the modified Goldblatt two-kidneys, one clip (2K1C) for induction of hypertension. In group: II, the rats were left untreated whereas in group III, IV, and V: RVH-rats were treated for 6 weeks with low dose hibiscus (LDH), medium dose hibiscus (MDH), and high dose hibiscus (HDH) respectively.

Results: We found that the augmented pro-contractile response of the aortic rings was ameliorated secondary to the in-vivo treatment with HS dose dependently. The cyclophilin A (CyPA) protein levels positively correlated with the vascular adhesion molecule-1 (VCAM-1) and ERK1/2, which, in turn, contribute to the reactive oxygen species (ROS) production. Daily HS intake modified aortic renovation by enhancing the antioxidant capacity, restraining hypertrophy and fibrosis, downregulation of the metastasis associated lung adenocarcinoma transcript (MALAT1), and cyclophilin A (CyPA)/ERK1/2 levels.

Discussion: Adding to the multiple beneficial effects, HS aqueous extract was able to inhibit vascular smooth muscle cell proliferation induced by 2K1C model. Thus, adding more privilege for the utilization of the traditional herbal extracts to attenuate RVH-induced aortopathy.
Reduction of bioavailability and phytotoxicity effect of cadmium in soil by microbial-induced carbonate precipitation using metabolites of ureolytic bacterium Ochrobactrum sp. POC9
Authors: Zakrzewska Marta , Rzepa Grzegorz , Musialowski Marcin , Goszcz Aleksandra , Stasiuk Robert , Debiec-Andrzejewska Klaudia
Published: June 2023

The application of ureolytic bacteria for bioremediation of soil contaminated with heavy metals, including cadmium (Cd), allows for the efficient immobilization of heavy metals by precipitation or coprecipitation with carbonates. Microbially-induced carbonate precipitation process may be useful also in the case of the cultivation of crop plants in various agricultural soils with trace but legally permissible Cd concentrations, which may be still uptaken by plants. This study aimed to investigate the influence of soil supplementation with metabolites containing carbonates (MCC) produced by the ureolytic bacterium Ochrobactrum sp. POC9 on the Cd mobility in the soil as well as on the Cd uptake efficiency and general condition of crop plants (Petroselinum crispum). In the frame of the conducted studies (i) carbonate productivity of the POC9 strain, (ii) the efficiency of Cd immobilization in soil supplemented with MCC, (iii) crystallization of cadmium carbonate in the soil enriched with MCC, (iv) the effect of MCC on the physico-chemical and microbiological properties of soil, and (v) the effect of changes in soil properties on the morphology, growth rate, and Cd-uptake efficiency of crop plants were investigated. The experiments were conducted in soil contaminated with a low concentration of Cd to simulate the natural environmental conditions. Soil supplementation with MCC significantly reduced the bioavailability of Cd in soil with regard to control variants by about 27-65% (depending on the volume of MCC) and reduced the Cd uptake by plants by about 86% and 74% in shoots and roots, respectively. Furthermore, due to the decrease in soil toxicity and improvement of soil nutrition with other metabolites produced during the urea degradation (MCC), some microbiological properties of soil (quantity and activity of soil microorganisms), as well as the general condition of plants, were also significantly improved. Soil supplementation with MCC enabled efficient Cd stabilization and significantly reduced its toxicity for soil microbiota and plants. Thus, MCC produced by POC9 strain may be used not only as an effective Cd immobilizer in soil but also as a microbe and plant stimulators.
Assembly and analysis of the first complete mitochondrial genome of Punica granatum and the gene transfer from chloroplast genome
Authors: Lu Guilong , Zhang Kai , Que Youxiong , Li Yanfeng
Published: June 2023

Pomegranate (Punica granatum L.) is one of the oldest fruits with edible, medicinal and ornamental values. However, there is no report on the mitochondrial genome of pomegranate. In this study, the mitochondrial genome of P. granatum was sequenced, assembled and analyzed in detail, while the chloroplast genome was assembled using the same set of data. The results showed that the P. granatum mitogenome had a multi branched structure, using BGI + Nanopore mixed assembly strategy. The total genome length was 404,807 bp, with the GC content of 46.09%, and there were 37 protein coding genes, 20 tRNA genes and three rRNA genes. In the whole genome, 146 SSRs were identified. Besides, 400 pairs of dispersed repeats were detected, including 179 palindromic, 220 forward and one reverse. In the P. granatum mitochondrial genome, 14 homologous fragments of chloroplast genome were found, accounting for 0.54% of the total length. Phylogenetic analysis showed that among the published mitochondrial genomes of related genera, P. granatum had the closest genetic relationship with Lagerstroemia indica of Lythraceae. The 580 and 432 RNA editing sites were predicted on 37 protein coding genes of mitochondrial genome using BEDTools software and online website PREPACT respectively, but all were from C to U, of which ccmB and nad4 gene were most frequently edited, with 47 sites. This study provides a theoretical basis for understanding the evolution of higher plants, species classification and identification, and will also be useful for further utilization of pomegranate germplasm resources.
STOP1 and STOP1-like proteins, key transcription factors to cope with acid soil syndrome
Authors: Li Xinbo , Tian Yifu
Published: June 2023

Acid soil syndrome leads to severe yield reductions in various crops worldwide. In addition to low pH and proton stress, this syndrome includes deficiencies of essential salt-based ions, enrichment of toxic metals such as manganese (Mn) and aluminum (Al), and consequent phosphorus (P) fixation. Plants have evolved mechanisms to cope with soil acidity. In particular, STOP1 (Sensitive to proton rhizotoxicity 1) and its homologs are master transcription factors that have been intensively studied in low pH and Al resistance. Recent studies have identified additional functions of STOP1 in coping with other acid soil barriers: STOP1 regulates plant growth under phosphate (Pi) or potassium (K) limitation, promotes nitrate (NO3-) uptake, confers anoxic tolerance during flooding, and inhibits drought tolerance, suggesting that STOP1 functions as a node for multiple signaling pathways. STOP1 is evolutionarily conserved in a wide range of plant species. This review summarizes the central role of STOP1 and STOP1-like proteins in regulating coexisting stresses in acid soils, outlines the advances in the regulation of STOP1, and highlights the potential of STOP1 and STOP1-like proteins to improve crop production on acid soils.
Multi-generation study of heavy ion beam-induced mutations and agronomic trait variations to accelerate rice breeding
Authors: Ren Weibin , Wang He , Du Yan , Li Yan , Feng Zhuo , Zhou Xinhui , Kang Guisen , Shu Qingyao , Guo Tao , Guo Huijun , Yu Lixia , Jin Wenjie , Yang Fu , Li Jingpeng , Ma Jianzhong , Li Wenjian , Xu Chaoli , Chen Xia , Liu Xiao , Yang Chenan , Liu Luxiang , Zhou Libin
Published: June 2023

Heavy ion beam (HIB) is an effective physical mutagen that has been widely used in plant mutational breeding. Systemic knowledge of the effects caused by different HIB doses at developmental and genomic levels will facilitate efficient breeding for crops. Here we examined the effects of HIB systematically. Kitaake rice seeds were irradiated by ten doses of carbon ion beams (CIB, 25 – 300 Gy), which is the most widely used HIB. We initially examined the growth, development and photosynthetic parameters of the M1 population and found that doses exceeding 125 Gy caused significant physiological damages to rice. Subsequently, we analyzed the genomic variations in 179 M2 individuals from six treatments (25 – 150 Gy) via whole-genome sequencing (WGS). The mutation rate peaks at 100 Gy (2.66×10-7/bp). Importantly, we found that mutations shared among different panicles of the same M1 individual are at low ratios, validating the hypothesis that different panicles may be derived from different progenitor cells. Furthermore, we isolated 129 mutants with distinct phenotypic variations, including changes in agronomic traits, from 11,720 M2 plants, accounting for a 1.1% mutation rate. Among them, about 50% possess stable inheritance in M3. WGS data of 11 stable M4 mutants, including three lines with higher yields, reveal their genomic mutational profiles and candidate genes. Our results demonstrate that HIB is an effective tool that facilitates breeding, that the optimal dose range for rice is 67 – 90% median lethal dose (LD50), and that the mutants isolated here can be further used for functional genomic research, genetic analysis, and breeding.
Balancing benefits and risks in lung cancer therapies: patient preferences for lung cancer treatment alternatives
Authors: Oliveri Serena , Lanzoni Lucilla , Veldwijk Jorien , de Wit G. Ardine , Petrocchi Serena , Janssens Rosanne , Schoefs Elise , Smith Meredith Y. , Smith Ian , Nackaerts Kristiaan , Vandevelde Marie , Louis Evelyne , Decaluwé Herbert , De Leyn Paul , Declerck Hanne , Petrella Francesco , Casiraghi Monica , Galli Giulia , Garassino Marina Chiara , Girvalaki Charis , Huys Isabelle , Pravettoni Gabriella
Published: June 2023

Background

In the treatment of Non-Small Cell Lung Cancer (NSCLC) the combination of Immuno- Oncotherapy (IO) and chemotherapy (CT) has been found to be superior to IO or CT alone for patients’ survival. Patients and clinicians are confronted with a preference sensitive choice between a more aggressive treatment with a greater negative effect on quality of life versus alternatives that are less effective but have fewer side effects.

Objectives

The aims of this study were to: (a) quantify patients’ preferences for relevant attributes related to Immuno-Oncotherapy treatment alternatives, and (b) evaluate the maximum acceptable risk (MAR)/Minimum acceptable benefit (MAB) that patients would accept for treatment alternatives.

Methods

An online preference survey using discrete-choice experiment (DCE) was completed by NSCLC patients from two hospitals in Italy and Belgium. The survey asked patients’ preferences for five patient- relevant treatment attributes. The DCE was developed using a Bayesian D-efficient design. DCE analyses were performed using mixed logit models. Information regarding patient demographics, health literacy, locus of control, and quality of life was also collected.

Results

307 patients (158 Italian, 149 Belgian), stage I to IV, completed the survey. Patients preferred treatments with a higher 5-year survival chance as the most important attribute over all the other attributes. Preference heterogeneity for the attribute weights depended on health literacy, patients’ age and locus of control. Patients were willing to accept a substantially increased risks of developing side effects in exchange for the slightest increase (1%) in the chance of surviving at least 5 years from the diagnosis of cancer. Similarly, patients were willing to accept a switch in the mode of administration or complete loss of hair to obtain an increase in survival.

Conclusion

In this study, the proportion of respondents who systematically preferred survival over all other treatment attributes was particularly high. Age, objective health literacy and locus of control accounted for heterogeneity in patients’ preferences. Evidence on how NSCLC patients trade between survival and other NSCLC attributes can support regulators and other stakeholders on assessing clinical trial evidence and protocols, based on patients’ conditions and socio-demographic parameters.
Association between the BDNF Val66Met polymorphism and major depressive disorder: a systematic review and meta-analysis
Authors: Wang Yuxia , Li Ou , Li Nana , Sha Zhongwei , Zhao Zhenghao , Xu Jian
Published: June 2023

Study objectives

This meta-analysis analytically reviewed recent studies concerning the potential associations between the brain-derived neurotrophic factor (BDNF) Val66Met polymorphism and susceptibility to major depressive disorder (MDD), with subgroup analyses for race and age.

Methods

Relevant case-control studies were systematically searched for in PubMed, Embase, the Web of Science, China National Knowledge Infrastructure (CNKI), Wanfang, and Sinomed databases. A total of 24 studies were finally identified to have reported outcomes including alleles, dominant genes, recessive genes, homozygosity, and heterozygosity. Subgroup meta-analyses were performed based on participant age and ethnicity. Publication bias was represented by funnel plots. All meta-analyses of the randomized controlled trials included for evaluation were performed using RevMan5.3 software.

Results

The findings revealed no significant association between BDNF Val66Met polymorphism and MDD. However, the Met allele was found to be associated with genetic susceptibility to MDD among white populations on subgroup analysis (OR = 1.25, 95% CI: 1.05–1.48, P = 0.01). In the genetic model, dominant (OR = 1.40, 95% CI: 1.18–1.66, P = 0.0001), recessive (OR = 1.70, 95% CI: 1.05–2.78, P = 0.03), and homozygous (OR = 1.77, 95% CI: 1.08–2.88, P = 0.02) genes were all associated with MDD.

Conclusions

Despite the outcome limitations, this meta-analysis confirmed that the BDNF Val66Met polymorphism is a susceptibility factor for MDD in white populations.
Effect of agility ladder training with a cognitive task (dual task) on physical and cognitive functions: a randomized study
Authors: Castillo de Lima Vivian , Castaño Luz Albany Arcila , Sampaio Ricardo Aurélio Carvalho , Sampaio Priscila Yukari Sewo , Teixeira Camila Vieira Ligo , Uchida Marco Carlos
Published: June 2023

Introduction

Agility training (AT) is used to improve neuromuscular performance and dynamic balance, which are crucial for the physical function of older adults. Activities of daily living, which decrease with age, involve tasks that simultaneously require motor, and cognitive abilities and can be considered dual tasks.

Methods

This study investigates a training program's physical and cognitive effects using an agility ladder on healthy older adults. This program consisted of 30-min sessions twice per week and lasted for 14 weeks. The physical training included four different sequences with progressive difficulty levels, while the cognitive training (CT) included different verbal fluency (VF) tasks for each physical task. Sixteen participants (mean age of 66.9 ± 5.0 years) were allocated to two groups: AT alone (AT) and dual-task training (AT combined with CT [AT + CT]). Assessments were performed before and after 14 weeks of interventions using physical functional tests (e.g., Illinois agility test, five times sit-to-stand test, timed up and go [TUG], and one-leg stand) and cognitive tests (cognitive TUG, verbal fluency, attention, and scenery picture memory test).

Results

After this period, both groups had significant differences in physical performance, muscle power, agility, static and dynamic balance, and short-term memory, whereas only the AT + CT group improved phonological verbal fluency, executive function (TUG combined with a cognitive task), attention (trail-making test-B), and short-term memory (scenery picture memory test).

Conclusion

Indicating that only the group that received direct cognitive training had better enhanced cognitive function.

Clinical trial registration

www.ClinicalTrials.gov, identifier: RBR-7t7gnjk.
Epidemiological characteristics and transmission dynamics of the COVID-19 outbreak in Hohhot, China: a time-varying SQEIAHR model analysis
Authors: Ma Yifei , Xu Shujun , Luo Yuxin , Qin Yao , Li Jiantao , Lei Lijian , He Lu , Wang Tong , Yu Hongmei , Xie Jun
Published: June 2023

Background

On September 28, 2022, the first case of Omicron subvariant BF.7 was discovered among coronavirus disease 2019 (COVID-19) infections in Hohhot, China, and then the epidemic broke out on a large scale during the National Day holiday. It is imminently necessary to construct a mathematical model to investigate the transmission dynamics of COVID-19 in Hohhot.

Methods

In this study, we first investigated the epidemiological characteristics of COVID-19 cases in Hohhot, including the spatiotemporal distribution and sociodemographic distribution. Then, we proposed a time-varying Susceptible-Quarantined Susceptible-Exposed-Quarantined Exposed-Infected-Asymptomatic-Hospitalized-Removed (SQEIAHR) model to derive the epidemic curves. The next-generation matrix method was used to calculate the effective reproduction number (Re). Finally, we explored the effects of higher stringency measures on the development of the epidemic through scenario analysis.

Results

Of the 4,889 positive infected cases, the vast majority were asymptomatic and mild, mainly concentrated in central areas such as Xincheng District. People in the 30–59 age group primarily were affected by the current outbreak, accounting for 53.74%, but females and males were almost equally affected (1.03:1). Community screening (35.70%) and centralized isolation screening (26.28%) were the main ways to identify positive infected cases. Our model predicted the peak of the epidemic on October 6, 2022, the dynamic zero-COVID date on October 15, 2022, a number of peak cases of 629, and a cumulative number of infections of 4,963 (95% confidential interval (95%CI): 4,692 ~ 5,267), all four of which were highly consistent with the actual situation in Hohhot. Early in the outbreak, the basic reproduction number (R0) was approximately 7.01 (95%CI: 6.93 ~ 7.09), and then Re declined sharply to below 1.0 on October 6, 2022. Scenario analysis of higher stringency measures showed the importance of decreasing the transmission rate and increasing the quarantine rate to shorten the time to peak, dynamic zero-COVID and an Re below 1.0, as well as to reduce the number of peak cases and final affected population.

Conclusion

Our model was effective in predicting the epidemic trends of COVID-19, and the implementation of a more stringent combination of measures was indispensable in containing the spread of the virus.
A qualitative inquiry of experiences of HIV-related stigma and its effects among people living with HIV on treatment in rural Kilifi, Kenya
Authors: Wanjala Stanley W. , Nyongesa Moses K. , Mapenzi Rachael , Luchters Stanley , Abubakar Amina
Published: June 2023

Background

The pervasiveness of HIV-related stigma and discrimination, and its consequences on HIV prevention and treatment, have been well documented. However, little is known about the lived experiences of HIV-related stigma and its effects among the general adult population living with HIV in rural African settings. This study set out to explore this knowledge gap.

Methods

From April to June 2018, we conducted in-depth interviews with a convenience sample of 40 adults living with HIV aged 18–58 years in Kilifi, Kenya. A semi-structured interview guide was used to explore experiences of HIV-related stigma and its impact on these adults. A framework approach was used to analyze the data using NVIVO 11 software.

Results

Participants reported experiences of HIV-related stigma in its various forms (anticipated, perceived, internalised, and enacted), as well as its effects on HIV treatment and social and personal spheres. The internalisation of stigma caused by enacted stigma impacted care-seeking behavior resulting in worse overall health. Anxiety and depression characterized by suicidal ideation were the results of internalised stigma. Anticipated stigma prompted HIV medication concealment, care-seeking in remote healthcare facilities, and care avoidance. Fewer social interactions and marital conflicts resulted from perceived stigma. Overall, HIV-related stigma resulted in partial and non-disclosure of HIV seropositivity and medication non-adherence. At a personal level, mental health issues and diminished sexual or marital prospects (for the unmarried) were reported.

Conclusion

Despite high awareness of HIV and AIDS among the general population in Kenya, adults living with HIV in rural Kilifi still experience different forms of HIV-related stigma (including self-stigma) that result in a raft of social, personal, and HIV-treatment-related consequences. Our findings underscore the urgent need to reevaluate and adopt more effective strategies for implementing HIV-related anti-stigma programs at the community level. Addressing individual-level stigma will require the design of targeted interventions. To improve the lives of adults living with HIV in Kilifi, the effects of HIV-related stigma, particularly on HIV treatment, must be addressed.
Examining human-animal interactions and their effect on multidimensional frailty in later life: a scoping review
Authors: Taeckens Ashley , Corcoran Mary , Wang Kaipeng , Morris Kevin N.
Published: June 2023

This scoping review sought to compile outcomes associated with any human-animal interaction study regarding adults aged 50 and older in any living context and concerning a multidimensional (i.e., physical, psychological, cognitive, and social) perspective of frailty. Despite our best attempts at incorporating the broadest inclusion criteria possible, only four articles were relevant to this review. Participants across the included studies were rural, community-dwelling Japanese or Chinese individuals aged 60 years and older. Thematic analysis of reported results includes dog ownership as a protective factor regarding frailty, the interconnected health effects of pet ownership, and increased meaning and purpose through pet ownership implications. More research is needed globally to determine how human-animal interactions may moderate frailty comprehensively, as well as the efficacy and appropriateness of these interactions or interventions in older adult populations and across cultural boundaries.
Multilevel logistic regression analysis of factors associated with delivery care service utilization among childbearing women in Ethiopia
Authors: Gebrekrstos Zeru Naod , Bedada Tolessa Dechasa , Abdisa Fufa Jaleta , Girma Fufa Bonsa
Published: June 2023

Delivery service utilization is one of the key and proven interventions to reduce maternal death during childbearing. In Ethiopia, the utilization of health facilities for delivery service is still at a lower level. This study intends to model the determinant factors for the delivery care service utilization of childbearing mothers in Ethiopia using the 2016 Ethiopian demographic and health survey data. A cross-sectional study design was selected to assess factors associated with delivery care among mothers who had at least one child in the last 5 years before the survey aged 15–49 years in the data. Among these eligible mothers, 3,052 (27.7%) mothers had received delivery service care from health professionals. The results of multilevel logistic regression indicated that those at age 35–49 years (AOR = 0.7808, 95% CI: 0.5965–1.1132), an urban place of residence (AOR = 5.849 95% CI: 4.2755–8.0021), woman's higher level of education (AOR = 3.484, 95% CI: 2.0214–6.0038) and partner's higher educational level (AOR = 1.9335, 95% CI: 3,808–2.07352), household wealth index (AOR = 1.99, 95% CI: 1.724–2.3122), most every day exposed to mass media (AOR = 3.068, 95% CI: 1.456–6.4624), 2–4 birth order number (AOR = 0.604, 95% CI: 0.51845–1.4213), using contraceptive type (AOR = 1.4584, 95% CI: 1.2591–1.6249) and visiting more than 4 antenatal care visits (AOR = 7.574, 95% CI: 6.4824–8.84896) were more likely to give birth at a health facility compared to their counterparts. The woman's and partner's educational level, household wealth index, exposure to mass media and number of antenatal care visits had a positive association with delivery assistance whereas birth order had a negative association. The findings of this study were valuable implications to support strategies and interventions to address delivery care service in Ethiopia.
Consumers behavior, attitudes, and beliefs regarding baobab (Adansonia digitata L.) fruit and pulp consumption in Sudan
Authors: Saeed Hassabelrasoul A. M. , Adam Yahia Omar , Donkor Emmanuel , Mithöfer Dagmar
Published: June 2023

There are growing number of empirical studies on the baobab value chain in Sub-Saharan Africa. Most studies focus on nutritional composition, traditional uses, the distribution of baobab trees, and collection. However, there are few studies on the marketing of baobab fruit pulp, especially on consumer behavior, attitudes, and beliefs regarding baobab fruit pulp. In this context, our study aims to explore consumer behavior, attitudes and beliefs regarding baobab fruit pulp. We also evaluate the market development potential of baobab fruit pulp in selected markets in El Obeid and Khartoum in Sudan. The study employs a mixed methods approach that includes a survey (N = 499), focus group discussion (N = 16), stakeholder interviews (N = 2), and a SWOT analysis. The study shows that the consumption of baobab fruit pulp is common among Sudanese consumers. Consumers also show strong positive attitudes and beliefs, as well as social support for baobab consumption. Nutritional and health benefits tend to drive consumer interest in baobab fruit pulp. The study also identified high demand, export opportunities, and extraction of ingredients from baobab as opportunities to develop baobab markets. However, a lack of quality control, regulations, poor distribution, and insufficient fruit supply may limit exploration of these opportunities. Therefore, it is crucial to raise knowledge of the nutritional and functional properties of baobab fruit, as well as its ability to fight health-related diseases, to further develop local markets. Processors must also improve the quality and safety of their goods. Policymakers must also create a regulatory structure that supports Sudan’s baobab value chain.
Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis
Authors: Prasolova Olga , Krylova Ekaterina , Bogomazova Alexandra , Soltynskaya Irina , Sklyarov Oleg , Gordeeva Veronika , Timofeeva Irina , Motorygin Anton , Panin Alexander
Published: June 2023

Over the past 10 years, immunization of cattle in Russia has been performed using vaccines from Brucella abortus strains 82, 19 and 75/79. To prevent brucellosis in small ruminants, two vaccines have been used, from the Brucella melitensis strain REV-1 and the B. abortus strain 19; note that twice as many animals have been immunized with the former vaccine than with the latter vaccine. The disadvantage of using these preparations is the formation of prolonged post-vaccination seropositivity, which is especially pronounced in animals after immunization with vaccines from B. abortus strain 19 and B. melitensis strain REV-1. This study aims to perform the whole genome sequencing of Brucella vaccine strains from the Russian collection. A bioinformatics analysis of the genomic data proved that the vaccine strains 75/79AB, 82, R-1096, and the KV 17/100 belong to ST-2, 104 M to ST-1, KV 13/100 to ST-5. This analysis allowed us to characterize vaccine strains’s phylogenetic relationships and to prove the close relation of vaccine strains 75/79AB, 82, R-1096. Also, we defined candidate mutations in genes pmm, wbdA, wbkA, wboA, and eryB, which could be responsible for the attenuated virulence of vaccine strains. The complete genomic sequences of B. abortus strains make further studies of bacterial pathogenicity determinants and virulence phenotype feasible, as well as their use in quality control of animal medicines.