bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study of the Influence of Music on Audiences’ Cognition of Animation
Authors: Wu Jun , Wu Jiede , Cheng Chien-Wen , Shih Chang-Chieh , Lin Po-Hsien
Published: November 2021

How do animation directors and music composers integrate personal creativity and expression into their work, and how do audiences understand and appreciate it as being important and worthy of discussion? This study explores the influence of music on audiences’ cognition of animation by using both quantitative and qualitative methods. Scholars specializing in aesthetics and music have conducted much research on music aesthetics and music itself. In recent years, further studies on music and film have also been carried out. However, there is a lack of research regarding audiences’ cognition of music in animation. This study focuses on the popular form of sand animation and provides insights into audiences’ cognition differences and preferences in order to uncover the core factors. The findings are that: (1) the audience perceived more consistent and subtle differences in the use of musical instruments, rhythm cadence and video–audio fit; there were also obvious differences in the perceptions of vocal skills, performance skills and musical style as well as emotional transmission; (2) three aspects of the audiences’ evaluation of an animation were affected by music: creativity, cultural meaning and preferences. The seven elements that constitute animation music (use of orchestration, vocal skills, musical style, rhythm cadence, performance techniques, emotional transmission and video–audio fit) exerted varying degrees of influence on the audiences’ evaluation of the animation film. Amongst these, video–audio fit was found to be the most important element, as it simultaneously affected the audiences’ evaluation in terms of creativity, cultural meaning and preferences; (3) audiences of different ages and professional backgrounds showed significant differences in evaluating animation films in terms of creativity, culture and preference; and (4) differences in music had a significant impact on audiences’ perceptions and evaluations of 10 facets of animation films, including the story content, role identification and spiritual fit.
Technical–tactical skill assessments in small-sided soccer games: A scoping review
Authors: Klingner Fabian C , Huijgen Barbara CH , Den Hartigh Ruud JR , Kempe Matthias
Published: November 2021

Skill assessments are essential to elite soccer coaches and clubs, to provide an evidence-based approach to player evaluation. Valid methods thereby support talent identification and development procedures (e.g. scouting and training strategies). However, it remains a complex challenge. Small-sided games have emerged as a promising tool, due to high ecological validity. Until now, no review has focused on their discriminative power. Therefore, we aimed to investigate whether technical–tactical skill assessments of small-sided games can discriminate between individual players and between teams of different skill levels (i.e. higher vs. lower playing levels and older vs. younger players) in soccer. A scoping review of PubMed, Web of Science, and MEDLINE databases was performed according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews guidelines. A total of 23 studies were included, all but one of which showed at least good methodological quality (i.e. > 50% score in quality assessment). For technical skills, small-sided games indicate discriminative power for passing skills, but only when comparing players of different playing levels, as supported by two of the included studies. Tactical categories, such as movement variability and decision-making, were more pronounced in higher level and more experienced players. However, the most conclusive finding among individual skill assessments was that the technical–tactical overall performance (a total score comprised of different subcategories) of individual players showed a positive relation to skill level in three studies. Team performance assessments in small-sided games showed that older and higher level teams mainly distinguish themselves from less-skilled peers by using the available space more efficiently. With the influence of different assessment instruments and several small-sided games modifications in mind, it may be concluded that technical–tactical skills in small-sided games can discriminate between players and teams of different skill levels. An interesting future avenue is to examine a more consistent approach to skill assessments in small-sided games, which can warrant their use for scouting and talent identification purposes.
Environmental crimes and green criminology in Bangladesh
Authors: Sharif Sherajul Mustajib , Uddin Md. Kamal
Published: November 2021

Bangladesh is an environmentally vulnerable country, where environmental crimes are massive and common. However, the environmental crime prevention mechanism in the country is very weak, and traditional policing is utilized to stop these crimes. Therefore, the green criminological approach to prevent environmental crimes in Bangladesh is underdeveloped in many ways, with a total absence of the green policing model. Hence, this study focuses on attaining a critical understanding of environmental crimes in Bangladesh by exploring the key underlying factors of environmental crimes. It also attempts to contribute to the environmental crime prevention mechanism by recommending a green policing model, while identifying the key weaknesses of the existing environmental crime prevention approach. This article implements the qualitative technique of data collection, and the analysis is based on an in-depth interview of 25 respondents, belonging to different categories of stakeholders, and participant observation. It also analyses the content of newspapers to understand the patterns of environmental crimes in Bangladesh. This article finds that environmental crimes are propagated by several political, economic, institutional, and social elements, such as the political affiliation of criminals, economic profit from natural resources, absence of institutional collaboration, and lack of social consciousness. The issue has become further aggravated due to the weakness of the crime prevention mechanism. Thus, the findings of this study suggest that environmental crimes in Bangladesh should be considered and understood from green criminological perspectives and the development of a green policing model would be effective in reducing environmental crimes in the country.
The German Multiple Sclerosis and Pregnancy Registry: rationale, objective, design, and first results
Authors: Thiel Sandra , Ciplea Andrea I. , Gold Ralf , Hellwig Kerstin
Published: November 2021

Objectives:

Multiple sclerosis (MS) and neuromyelitis optica spectrum disorders (NMOSD) predominantly affect women of reproductive age. During the last few decades many disease-modifying therapies (DMTs) have been approved. It is therefore important to provide epidemiological structures for the collection of safety information on exposed pregnancies. Data on disease activity after withdrawal of DMTs are in high demand especially as severe relapses have been described after ceasing highly effective DMTs. Although breastfeeding is recommended, it is still unclear if the early reintroduction, especially of highly effective DMTs, has a beneficial effect on postpartum relapse risk or a combination of both, however safety data are lacking.

Methods:

The German MS and Pregnancy Registry (DMSKW) is a nationwide, observational, cohort study of pregnant women with MS or NMOSD, founded in 2006. As the study procedure has undergone important adaptation in recent years, described here is the updated methodology including data source and acquisition as well as variables collected within the DMSKW.

Results:

As of December 2020, the DMSKW database comprises 2579 pregnancies, 2568 with MS and 11 with NMOSD. Women are enrolled at a median gestational week of 11 (range: 0.02–42.1), have a median postpartum follow up of 1.2 years (range: 0–9.2) with 76% of all pregnancies being exposed to a DMT, mostly in the first trimester. Spontaneous abortion and preterm birth occurred in 7% and 10%, respectively; 19% of all women suffered from at least one relapse during pregnancy, with a minimum of 6% during the third trimester of pregnancy.

Conclusion:

The DMSKW is a valuable structure in providing safety data on drug exposure during pregnancy and lactation in combination with information on disease activity up to 6 years postpartum. This article will be the reference for describing the methods of future publications from the DMSKW.
A multi-sided market of personal data resource allocation: An empirical study of China’s car-hailing platform
Authors: Huang Lei , Ladikas Miltos , He Guangxi , Hahn Julia , Schippl Jens
Published: November 2021

The current rapid development of online car-hailing services creates a serious challenge to the existing paradigm of market governance and antitrust policy. However, the debate on the market structure of the car-hailing platform requires more empirical evidence to uncover its functions. This research adopts an interdisciplinary methodology based on computer science and economics, and including software reverse engineering tools applied to the interoperability of the terminal application and resource allocation model, to demonstrate the topological market structure of personal data resources allocation in China’s car-hailing industry. Within the discussion of the hybrid nature of technology and economy, the analysis results clearly show that China’s car-hailing platform services present a multi-sided market structure when seen from the perspective of personal data resource allocation. Personal data resource (PDR), that is considered an essential market resource, is applied as an asset transferred unhindered between platforms via the application programming interface, and thus, creating a new market allocation mechanism. The connection between the car-hailing platforms and social media platforms is an essential aspect of the market competition in the domain. As applications of online platforms increase in the global context, this research offers a new perspective in personal data resource allocation with implications for the governance of the platform economy.
Politics, Power and a Pandemic: Searching for Information and Accountability During a Twitter Infodemic
Authors: LaPoe Benjamin R. , Carter Olson Candi S. , LaPoe Victoria L. , Jain Parul , Woellert Allyson , Long Aaron
Published: November 2021

During the early weeks of the U.S. COVID-19 pandemic, society was battling an infodemic–defined as a “tsunami” of online misinformation. Through the lens of mediatization theory, this article examines 800,000 tweets to understand social media information and misinformation related to the COVID-19. Through multi-layered analysis, this article details prominent key words discussed on Twitter connected to pandemic trending hashtags in early-to-mid March 2020: #Covid19 and #Coronavirus. The most prominent word themes included: novelty of this virus and associated uncertainty and the spread of misinformation; severity and widespread reach of the virus; call for collective action; and expectations relative to government action. The article explains these findings through mediatization theory, applying how technology influences social media discussions.
COVID-19 Pandemic Between Severity Facts and Prophylaxis
Authors: Liu Hui , Zhu Wenxia , Wu Yilin , Jiang Caini , Huo Lili , Belal Amany
Published: November 2021

Before COVID-19, many viruses have infected humans, so what caused COVID-19 to be considered as a pandemic? COVID-19 belongs to the coronavirus family that includes severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS). This family has caused a large number of deaths all over the world. How risky is the novel coronavirus? People and their careers were disrupted, and many businesses all over the world are now closed. From here, it seems to us that this virus is something that can make people feel afraid. In this article, we will try to understand the severity of this virus, and then disclose the available ways to confront it and ways that might improve the ability to face it, either now or in the future. Upon comparing COVID-19 with seasonal flu, we have found that COVID-19 is about 10 times more deadly, although it is not the most infectious virus. In this review, we will discuss how healthy nutrition and lifestyle may help to prevent and treat diseases, and especially COVID-19. We will focus on how to follow healthy nutrition habits and lifestyles to stop the dangers of COVID-19.
In Vitro ACE2 and 5-LOX Enzyme Inhibition by Menthol and Three Different Mint Essential Oils
Authors: Demirci Fatih , Karadağ Ayşe Esra , Biltekin Sevde Nur , Demirci Betül
Published: November 2021

Mentha arvensis L., M. citrata L., and M. spicata L. (family Lamiaceae) essential oils, and their characteristic constituent, menthol, were evaluated in vitro for angiotensin converting enzyme 2 (ACE2) and 5-lipoxygenase (5-LOX) enzyme inhibitory activity. The chemical compositions of M. arvensis, M. citrata, and M. spicata essential oils were analysed both by GC-FID, and GC/MS; 82.0%, 38.1%, and 0.4% menthol were identified, respectively. M. spicata essential oil contained 88.2% carvone as its major component.

The enzyme inhibitory activities of the essential oils were evaluated using a fluorometric multiplate based enzyme inhibition kit; the ACE2 inhibitions produced by M. arvensis, M. citrata, and M. spicata essential oils were 33%, 22%, and 73%, while the 5-LOX inhibitions were 84%, 79%, and 70%, respectively. In addition, menthol also showed remarkable ACE2 inhibition of 99.8%, whereas the 5-LOX inhibition was 79.9%. As a result, menthol and the three different mint essential oils may have antiviral potential applications against coronaviruses due to their ACE2 enzyme inhibition and anti-inflammatory features. However, further in vivo studies are needed to confirm the safety and efficacy.
Framing the Global Youth Climate Movement: A Qualitative Content Analysis of Greta Thunberg’s Moral, Hopeful, and Motivational Framing on Instagram
Authors: Molder Amanda L. , Lakind Alexandra , Clemmons Zoe E. , Chen Kaiping
Published: November 2021

Climate change is a critical global problem that requires immediate action to mitigate its effects. In recent years, youth climate activists have mobilized worldwide protests to demand action, using social media platforms to communicate and broadcast their message. This study examines Greta Thunberg's rise to global prominence through an analysis of her first year and a half of Instagram posts from June 2018 to January 2020, including visual and textual elements. First, we explore how climate change is communicated on social media by youth activists, and then examine these concepts through the unique case of Thunberg’s Instagram. Then, through qualitative content analysis, this study elucidates her communication strategy by applying the concept of framing to unpack how she frames climate change as a moral and ethical issue, uses an emotional appeal of hope, and visually frames motivational collective action to mobilize her audience. Finally, we discuss the implications of our findings to explore the complexities of communicating climate change through social media and how Thunberg's activism on Instagram may provide an example for future generations.
How and when do mobile media demands impact well-being? Explicating the Integrative Model of Mobile Media Use and Need Experiences (IM3UNE)
Authors: Schneider Frank M. , Lutz Sarah , Halfmann Annabell , Meier Adrian , Reinecke Leonard
Published: November 2021

Using mobile media can be both detrimental and beneficial for well-being. Thus, explaining how and when they elicit such effects is of crucial importance. To explicate boundary conditions and processes for digital well-being, this article introduces the Integrative Model of Mobile Media Use and Need Experiences (IM³UNE). Instead of assuming mobile media to be pathogenic, the IM³UNE offers a salutogenic perspective—it focuses on how we can stay healthy when using mobile media ubiquitously in daily life. More specifically, the model assumes that both the satisfaction and the frustration of basic psychological needs are key underlying mechanisms linking demanding mobile media use to well-being. However, the impact of these mechanisms is contingent on how users perceive, appraise, act on, and make sense of mobile media demands according to their global orientation to life (i.e., their sense of coherence, SOC). Integrating prior work, we theoretically link mindfulness, self-control, and meaningfulness to SOC's central facets, arguing that they represent crucial personal resources required to cope with mobile media demands. Thus, the offers an integrative framework, guiding further research towards a more nuanced study of mobile media’s effect on well-being.
Eye-care utilization among a Canadian diabetic refugee population: retrospective cohort study of an interdisciplinary care model
Authors: Tanya Stuti M , He Bonnie , Aubrey-Bassler Christine
Published: November 2021

Introduction

Vision health is an important and underutilized health service among newly arrived refugees in Canada, yet the body of literature on eye-care delivery in this population is limited. The study objective was to identify patterns of eye-care utilization among refugee patients with type 2 diabetes mellitus (T2DM) in Newfoundland and Labrador (NL) under an interdisciplinary clinic model comprised of family physicians, eye-care providers, and settlement services.

Methods

This was a retrospective cohort study at the Memorial University Family Medicine clinic. All patients with a new T2DM diagnosis between 2015–2020 were included. Data were described using basic statistics and unpaired t-tests. This study received full ethics approval.

Results

Seventy-three (18 refugee, 55 non-refugee) patients were included. Refugees had a higher rate of referral to an eye-care provider ( p = 0.0475) and were more likely to attend their eye-care provider appointment than non-refugees ( p = 0.016). The time from diagnosis to referral was longer for refugees than non-refugees ( p = 0.0498). A trend towards longer time from referral to appointment attendance for refugees than non-refugees was noted ( p = 0.9069).

Discussion

Refugee patients had higher rates of referral to eye-care providers and utilization of eye-care services. However, refugees also experienced a longer time to access vision screening services suggesting possible gaps in accessible care delivery. This suggests that the interdisciplinary model of care may be effective in referring refugee patients for vision screening and there may be a role for increased collaboration across family physicians, eye-care providers, and settlement services to improve accessibility of vision screening services.
Recovery After ACL Reconstruction in Male Versus Female Adolescents: A Matched, Sex-Based Cohort Analysis of 543 Patients
Authors: Maguire Kathleen , Sugimoto Dai , Micheli Lyle J. , Kocher Mininder S. , Heyworth Benton E.
Published: November 2021

Background:

Differences in postoperative recovery after anterior cruciate ligament reconstruction (ACLR) between men and women have been demonstrated in the adult population. Sex-based differences have been incompletely investigated in adolescents, which represent the subpopulation most affected by ACL injury.

Purpose/Hypothesis:

The purpose of this study was to compare the 6-month postoperative functional recovery after ACLR between adolescent boys and girls. It was hypothesized that significant differences in postoperative strength, dynamic balance, and functional hop test performance would be seen between the sexes.

Study Design:

Cohort study; Level of evidence, 3.

Methods:

Included in this study were athletes aged 12 to 19 years with closed or closing growth plates who underwent ACLR with hamstring autograft between May 2014 and May 2018 at a single institution. All athletes had undergone strength and functional testing between 5 and 8 months postoperatively. Exclusion criteria were previous knee surgery (contralateral or ipsilateral knee), concomitant injury/surgery other than meniscal tear/repair, allograft supplementation, and incomplete medical records. The limb symmetry index (LSI) for strength (measured with handheld dynamometer), as well as dynamic Y-balance and functional hop test performance, was compared between groups. To account for differences in physical characteristics between the sexes, 1-way between-group multivariate analysis of covariance was used to analyze the data.

Results:

Overall, 543 patients (211 boys, 332 girls) were included. There was no significant difference in age, body mass index, incidence of concomitant meniscal pathology, use of regional anesthesia, or time to functional testing between cohorts. Female athletes demonstrated a statistically significantly greater deficit in quadriceps strength LSI compared with male athletes (boys, +3.4%; girls, –2.3%; P = .011). Both male and female athletes demonstrated 33% hamstring strength deficits, with no statistically significant sex-based differences in dynamic balance or functional hop testing.

Conclusion:

Female athletes demonstrated greater quadriceps strength deficits than male athletes at 6 months after ACLR with hamstring autograft. Severe hamstring strength deficits persisted in both male and female patients at this time point. The correlation of such deficits to risk of ACL retear warrants continued study in the adolescent population and may support a delay in return to sports, which has been suggested in the more recent literature.
Rule by law versus rule of law: A multimodal analysis of persuasion and legal ideologies in anti-corruption discourse in China
Authors: Yuan Chuanyou , He Yufei , Liu Yujie
Published: November 2021

The authors conduct a multimodal analysis of the anti-corruption discourse in China by employing the SFL genre theory and the SF-MDA approach. Anti-corruption discourse that popularizes the anti-corruption mechanism and educates the officials constitutes an important part of China’s anti-corruption campaign. This paper first presents a genre analysis of a corpus of 51 anti-corruption videos on the official public legal education website to examine how these videos are designed in their overall organization to achieve the persuasion purpose—alert officials to stay away from corruption. It is found that most anti-corruption videos are expositions that are embedded with different story genres and emphasize the negative consequence of corruption on one’s family. Using Multimodal Analysis Video software, the authors then analyze the different reader stances enacted through a range of multimodal resources in three representative anti-corruption videos. Based on the detailed multimodal analysis, the authors finally explain how the use of linguistic and visual resources in the videos realizes the underlying ideologies of rule by law and rule of law and future implications of this study.
Cosmetic outcomes of grafted tubularized incised plate urethroplasty in primary distal penile hypospadias: prospective comparative study with the classic Snodgrass repair
Authors: Ahmed Shabib , Noureldin Yasser A. , Sherif Hammoda , Zahran Ahmed , Omar Rabea
Published: November 2021

Background

To compare the outcomes between classic tubularized incised plate (C-TIP), known as Snodgrass urethroplasty, and grafted TIP (G-TIP) in the repair of primary distal penile hypospadias.

Methods

Parents of all children presented to our tertiary care institution with primary distal penile hypospadias were asked to participate in this study. Patients were equally randomized using closed envelope method into two groups; Group A underwent repair using G-TIP and Group B underwent repair using the C-TIP. Circumcised cases and/or cases with penile chordee > 30 degrees were excluded from this study. Urethral catheter was kept for 7 to 10 days after surgery. The success rate and cosmetic outcomes assessed by HOSE score were evaluated at 6-month postoperatively.

Results

A total of 55 patients were recruited in each group. One hundred and seven patients of the 110 patients (54 and 53 in groups A and B, respectively) were evaluated at 6-month postoperatively using HOSE score. All preoperative data were comparable in both groups. Success was documented in 49/54 patients (90.7%) in group A. The five failures were secondary to two cases of glans dehiscence and three cases of residual postoperative chordee. Whereas, success was documented in 48/53 patients (90.5%) in group B. Complications were: a case of meatal stenosis, three cases of fistula, and a case of combined meatal stenosis and fistula. The HOSE score was comparable between the two groups (15.4 ± 1.09 vs. 15.6 ± 0.55; p = 0.29). However, the operative time was statistically longer in the G-TIP compared with the C-TIP (91.4 ± 6.2 min vs. 85.2 ± 6.3 min; p < 0.001), respectively.

Conclusion

The G-TIP urethroplasty provided comparable results with C-TIP in terms of cosmoses, success rate, and complications. However, G-TIP was accompanied with significantly longer operative time.
Split it up and see: using proxies to highlight divergent inter-populational performances in aquaculture standardised conditions
Authors: Toomey Lola , Dellicour Simon , Kapusta Andrzej , Żarski Daniel , Buhrke Frederik , Milla Sylvain , Fontaine Pascal , Lecocq Thomas
Published: November 2021

Background

Considering wild inter-populational phenotypic differentiation can facilitate domestication and subsequent production of new species. However, comparing all populations across a species range to identify those exhibiting suitable key traits for aquaculture (KTA; i.e. important for domestication and subsequent production) expressions is not feasible. Therefore, proxies highlighting inter-populational divergences in KTA are needed. The use of such proxies would allow to identify, prior to bioassays, the wild population pairs which are likely to present differentiations in KTA expressions in aquaculture conditions. Here, we assessed the relevance of three alternative proxies: (i) genetic distance, (ii) habitat divergence, and (iii) geographic/hydrologic distances. We performed this evaluation on seven allopatric populations ofPerca fluviatilisfor which divergences in KTA had already been shown.

Results

We showed differences in the correlation degree between the alternative proxy-based and KTA-based distance matrices, with the genetic proxy being correlated to the highest number of KTA. However, no proxy was correlated to all inter-populational divergences in KTA.

Conclusion

For future domestication trials, we suggest using a multi-proxy assessment along with a prioritisation strategy to identify population pairs which are of interest for further evaluation in bioassays.
Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohorts
Authors: Wu Zeni , Hullings Autumn G. , Ghanbari Reza , Etemadi Arash , Wan Yunhu , Zhu Bin , Poustchi Hossein , Fahraji Behnam Bagheri , Sakhvidi Mohammad Javad Zare , Shi Jianxin , Knight Rob , Malekzadeh Reza , Sinha Rashmi , Vogtmann Emily
Published: November 2021

Background

To initiate fecal and oral collections in prospective cohort studies for microbial analyses, it is essential to understand how field conditions and geographic differences may impact microbial communities. This study aimed to investigate the impact of fecal and oral sample collection methods and room temperature storage on collection samples for studies of the human microbiota.

Results

We collected fecal and oral samples from participants in two Iranian cohorts located in rural Yazd (n = 46) and urban Gonbad (n = 38) and investigated room temperature stability over 4 days of fecal (RNAlater and fecal occult blood test [FOBT] cards) and comparability of fecal and oral (OMNIgene ORAL kits and Scope mouthwash) collection methods. We calculated interclass correlation coefficients (ICCs) based on 3 alpha and 4 beta diversity metrics and the relative abundance of 3 phyla. After 4 days at room temperature, fecal stability ICCs and ICCs for Scope mouthwash were generally high for all microbial metrics. Similarly, the fecal comparability ICCs for RNAlater and FOBT cards were high, ranging from 0.63 (95% CI: 0.46, 0.75) for the relative abundance of Firmicutes to 0.93 (95% CI: 0.89, 0.96) for unweighted Unifrac. Comparability ICCs for OMNIgene ORAL and Scope mouthwash were lower than fecal ICCs, ranging from 0.55 (95% CI: 0.36, 0.70) for the Shannon index to 0.79 (95% CI: 0.69, 0.86) for Bray-Curtis. Overall, RNAlater, FOBT cards and Scope mouthwash were stable up to 4 days at room temperature. Samples collected using FOBT cards were generally comparable to RNAlater while the OMNIgene ORAL were less similar to Scope mouthwash.

Conclusions

As microbiome measures for feces samples collected using RNAlater, FOBT cards and oral samples collected using Scope mouthwash were stable over four days at room temperature, these would be most appropriate for microbial analyses in these populations. However, one collection method should be consistently since each method may induce some differences.
Identification of quantitative trait nucleotides and candidate genes for tuber yield and mosaic virus tolerance in an elite population of white guinea yam (Dioscorea rotundata) using genome-wide association scan
Authors: Agre Paterne A. , Norman Prince E. , Asiedu Robert , Asfaw Asrat
Published: November 2021

Background

Improvement of tuber yield and tolerance to viruses are priority objectives in white Guinea yam breeding programs. However, phenotypic selection for these traits is quite challenging due to phenotypic plasticity and cumbersome screening of phenotypic-induced variations. This study assessed quantitative trait nucleotides (QTNs) and the underlying candidate genes related to tuber yield per plant (TYP) and yam mosaic virus (YMV) tolerance in a panel of 406 white Guinea yam (Dioscorea rotundata) breeding lines using a genome-wide association study (GWAS).

Results

Population structure analysis using 5,581 SNPs differentiated the 406 genotypes into seven distinct sub-groups based delta K. Marker-trait association (MTA) analysis using the multi-locus linear model (mrMLM) identified seventeen QTN regions significant for TYP and five for YMV with various effects. The seveteen QTNs were detected on nine chromosomes, while the five QTNs were identified on five chromosomes. We identified variants responsible for predicting higher yield and low virus severity scores in the breeding panel through the marker-effect prediction. Gene annotation for the significant SNP loci identified several essential putative genes associated with the growth and development of tuber yield and those that code for tolerance to mosaic virus.

Conclusion

Application of different multi-locus models of GWAS identified 22 QTNs. Our results provide valuable insight for marker validation and deployment for tuber yield and mosaic virus tolerance in white yam breeding. The information on SNP variants and genes from the present study would fast-track the application of genomics-informed selection decisions in breeding white Guinea yam for rapid introgression of the targeted traits through markers validation.
Genome-wide association analysis uncovers the genetic architecture of tradeoff between flowering date and yield components in sesame
Authors: Sabag Idan , Morota Gota , Peleg Zvi
Published: November 2021

Background

Unrevealing the genetic makeup of crop morpho-agronomic traits is essential for improving yield quality and sustainability. Sesame (Sesamum indicum L.) is one of the oldest oil-crops in the world. Despite its economic and agricultural importance, it is an ‘orphan crop-plant’ that has undergone limited modern selection, and, as a consequence preserved wide genetic diversity. Here we established a new sesame panel (SCHUJI) that contains 184 genotypes representing wide phenotypic variation and is geographically distributed. We harnessed the natural variation of this panel to perform genome-wide association studies for morpho-agronomic traits under the Mediterranean climate conditions.

Results

Field-based phenotyping of the SCHUJI panel across two seasons exposed wide phenotypic variation for all traits. Using 20,294 single-nucleotide polymorphism markers, we detected 50 genomic signals associated with these traits. Major genomic region on LG2 was associated with flowering date and yield-related traits, exemplified the key role of the flowering date on productivity.

Conclusions

Our results shed light on the genetic architecture of flowering date and its interaction with yield components in sesame and may serve as a basis for future sesame breeding programs in the Mediterranean basin.
QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
Authors: Gutierrez Natalia , Torres Ana M.
Published: November 2021

Background

Ascochyta blight caused by Ascochyta fabae Speg. and broomrape (Orobanche crenata) are among the economically most significant pathogens of faba bean. Several QTLs conferring resistance against the two pathogens have been identified and validated in different genetic backgrounds. The aim of this study was to saturate the most stable QTLs for ascochyta and broomrape resistance in two Recombinant Inbred Line (RIL) populations, 29H x Vf136 and Vf6 x Vf136, to identify candidate genes conferring resistance against these two pathogens.

Results

We exploited the synteny between faba bean and the model species Medicago truncatula by selecting a set of 219 genes encoding putative WRKY transcription factors and defense related proteins falling within the target QTL intervals, for genotyping and marker saturation in the two RIL populations. Seventy and 50 of the candidate genes could be mapped in 29H x Vf136 and Vf6 x Vf136, respectively. Besides the strong reduction of the QTL intervals, the mapping process allowed replacing previous dominant and pedigree-specific RAPD flanking markers with robust and transferrable SNP markers, revealing promising candidates for resistance against the two pathogens.

Conclusions

Although further efforts in association mapping and expression studies will be required to corroborate the candidate genes for resistance, the fine-mapping approach proposed here increases the genetic resolution of relevant QTL regions and paves the way for an efficient deployment of useful alleles for faba bean ascochyta and broomrape resistance through marker-assisted breeding.
Sleep quality and emergence delirium in children undergoing strabismus surgery: a comparison between preschool- and school-age patients
Authors: Do Wangseok , Kim Hyo-Sung , Kim Seung Ha , Kang Hyunjong , Lee Dowon , Baik Jiseok , Lee Hyeon Jeong , Hong Jeong-Min
Published: November 2021

Background

Emergence delirium (ED) is common in pediatric patients undergoing general anesthesia with sevoflurane. Preoperative sleep quality is associated with the risk factors for ED. However, research on the relationship between sleep quality and ED is limited. We aimed to investigate the relationship between ED and preoperative sleep quality in pediatric patients undergoing strabismus surgery.

Methods

This clinical trial included pediatric patients aged 4–12 years who underwent elective strabismus surgery. The patients and their parents were questioned about the patients’ preoperative sleep quality using the Pittsburgh Sleep Quality Index (PSQI) questionnaire. For anesthesia induction, thiopental (5 mg/kg) and rocuronium (0.6 mg/kg) were used, and anesthesia was maintained with sevoflurane (minimum alveolar concentration, 1–1.5). After administration of a reversal drug, extubation was performed, and the patients were transferred to a post-anesthesia recovery unit. At 10 min after extubation, the degree of ED was measured using the pediatric anesthesia emergence delirium (PAED) and Watcha scale scores.

Results

Of the 62 enrolled patients, three pediatric patients were excluded. The overall incidence of ED was 22%. A total of 59 patients were divided into the two groups. The ED group and the non-ED group comprised 13 and 46 patients. Age, height and weight were significantly lower in the ED group than in the non-ED group. Preoperative PSQI and Watcha scale score were significantly higher in the ED group than in the non-ED group. Multivariate analysis showed that age (adjusted OR [95% CI]: 0.490 [0.290–0.828],p = 0.008) and preoperative PSQI score (adjusted OR [95% CI]: 2.149[1.224–3.771],p = 0.008) was associated with ED. In sub-group analysis, PAED scale and Watcha scale scores showed a moderate correlation with preoperative sleep quality in preschool-age patients.

Conclusion

In conclusion, the incidence of ED tended to be higher in younger age and poorer preoperative sleep quality in pediatric patients. In particular, the poorer sleep quality score was associated with higher incidence of ED in the preschool-age. Large-scale clinical studies and long-term follow-up studies on ED and sleep quality are required.

Trial registration

This study was registered with Clinicaltrials.gov (NCT03332407) at November 5th 2017.
Evaluation of the effect of clinical characteristics and intensive care treatment methods on the mortality of covid-19 patients aged 80 years and older
Authors: Oba Sibel , Altınay Mustafa , Salkaya Aysel , Türk Hacer Şebnem
Published: November 2021

Background

Older adults have an increased risk of mortality from Coronavirus disease 2019 (Covid-19). Despite the high number of publications on the topic of Covid-19 pandemic, few studies have focused on the intensive care treatments of Covid-19 patients aged 80 years and older.

The goal of our study is to investigate the effect of the intensive care treatments on the mortality of Covid-19 patients aged 80 years and older based on their clinical features, laboratory findings and the intensive care treatments methods.

Methods

The data of 174 patients aged 80 years and older treated from Covid-19 in intensive care unit were assessed retrospectively. The patients were divided into two groups as survivor and non-survivor. The effects of age, gender, length of stay, comorbid diseases, laboratory values, thoracic computed tomography findings, having invasive mechanical ventilation (IMV), high flow nasal cannula (HFNC) and/or non-invasive mechanical ventilation (NIMV), hemodiafiltration (HDF), anti-cytokines and plasma therapy on mortality have been investigated.

Results

The mean age and mean values of CRP, PCT, Ferritin, LDH were statistically significantly high in the non-survivor group. The mortality rate of the patients who had IMV was also statistically significantly higher compared to patients who had HFNC and/or NIMV. Albumin level and the rate of treatment with HFNC and/or NIMV were statistically significantly low in non-survivor group compared to the Survivor group.

Conclusion

ICU treatments may be beneficial for the Covid-19 patients aged 80 years and older. Increased age, high levels of CRP, PCT, ferritin, and having IMV are detected as poor outcome markers.
Monocyte distribution width (MDW) as a new tool for the prediction of sepsis in critically ill patients: a preliminary investigation in an intensive care unit
Authors: Polilli Ennio , Frattari Antonella , Esposito Jessica Elisabetta , Stanziale Andrea , Giurdanella Giuliana , Di Iorio Giancarlo , Carinci Fabrizio , Parruti Giustino
Published: November 2021

Background

Monocyte Distribution Width (MDW), a simple proxy marker of innate monocyte activation, can be used for the early recognition of sepsis along with Procalcitonin. This study explored the added value of MDW as an early predictor of ensuing sepsis in patients hospitalised in an Intensive Care Unit.

Methods

We performed an observational prospective monocentric study to estimate the analytical performance of MDW in detecting ensuing sepsis in a sample of consecutive patients assisted in an Intensive Care Unit for > 48 h for any reason. Demographic and clinical characteristics, past medical history and other laboratory measurements were included as potential predictors of confirmed sepsis in multivariate logistic regression.

Results

A total of 211 patients were observed, 129 of whom were included in the final sample due to the suspect of ensuing sepsis; of these, 74 (57%) had a confirmed diagnosis of sepsis, which was best predicted with the combination of MDW > 23.0 and PCT > 0.5 ng/mL (Positive Predictive Value, PPV: 92.6, 95% CI: 82.1–97.9). The best MDW cut-off to rule out sepsis was ≤20.0 (Negative Predictive Value, NPV: 86.4, 95% CI: 65.1–97.1). Multivariate analyses using both MDW and PCT found a significant association for MDW > 23 only (OR:17.64, 95% CI: 5.53–67.91).

Conclusion

We found that values of MDW > 23 were associated with a high PPV for sepsis, whereas values of MDW ≤ 20 were associated with a high NPV. Our findings suggest that MDW may help clinicians to monitor ICU patients at risk of sepsis, with minimal additional efforts over standard of care.
Oversampling and replacement strategies in propensity score matching: a critical review focused on small sample size in clinical settings
Authors: Bottigliengo Daniele , Baldi Ileana , Lanera Corrado , Lorenzoni Giulia , Bejko Jonida , Bottio Tomaso , Tarzia Vincenzo , Carrozzini Massimiliano , Gerosa Gino , Berchialla Paola , Gregori Dario
Published: November 2021

Background

Propensity score matching is a statistical method that is often used to make inferences on the treatment effects in observational studies. In recent years, there has been widespread use of the technique in the cardiothoracic surgery literature to evaluate to potential benefits of new surgical therapies or procedures. However, the small sample size and the strong dependence of the treatment assignment on the baseline covariates that often characterize these studies make such an evaluation challenging from a statistical point of view. In such settings, the use of propensity score matching in combination with oversampling and replacement may provide a solution to these issues by increasing the initial sample size of the study and thus improving the statistical power that is needed to detect the effect of interest. In this study, we review the use of propensity score matching in combination with oversampling and replacement in small sample size settings.

Methods

We performed a series of Monte Carlo simulations to evaluate how the sample size, the proportion of treated, and the assignment mechanism affect the performances of the proposed approaches. We assessed the performances with overall balance, relative bias, root mean squared error and nominal coverage. Moreover, we illustrate the methods using a real case study from the cardiac surgery literature.

Results

Matching without replacement produced estimates with lower bias and better nominal coverage than matching with replacement when 1:1 matching was considered. In contrast to that, matching with replacement showed better balance, relative bias, and root mean squared error than matching without replacement for increasing levels of oversampling. The best nominal coverage was obtained by using the estimator that accounts for uncertainty in the matching procedure on sets of units obtained after matching with replacement.

Conclusions

The use of replacement provides the most reliable treatment effect estimates and that no more than 1 or 2 units from the control group should be matched to each treated observation. Moreover, the variance estimator that accounts for the uncertainty in the matching procedure should be used to estimate the treatment effect.
Clinicopathological significance of HSP70 expression in gastric cancer: a systematic review and meta-analysis
Authors: Wang Xiaolu , Xie Li , Zhu Lijing
Published: November 2021

Background

Heat shock protein 70 (HSP70) has been associated with the clinicopathological characteristics and prognosis of many cancers types, implying that it is a potential cancer biomarker. However, no consensus has been reached regarding its clinicopathological and prognostic significance in patients with gastric cancer. To address this gap, we performed a systematic review and meta-analysis.

Methods

We searched PubMed, Embase, and the Cochrane Library for full-text literature according to the eligibility criteria. We used the odds ratio and hazard ratio as the suitable parameters to evaluate the clinicopathological and prognostic significance of HSP70. The statistical analysis was performed using STATA 15.0.

Results

After inclusion and exclusion of studies based on the eligibility criteria, data of 1,307 patients with gastric cancer from 9 studies were finally included. The pooled outcomes implied that HSP70 expression was significantly correlated with higher differentiation degrees, intestinal gastric cancer, and lymphovascular invasion but not with age, gender, depth of invasion,Helicobacter pyloriinfection, lymph node invasion, TNM stages, and metastasis. The pooled HR showed no significant correlation between HSP70 expression and overall survival of gastric cancer patients.

Conclusions

Our meta-analysis showed that HSP70 plays a complicated role in the development of gastric cancer. It may be directly engaged in tumour differentiation and distant invasion but cannot be considered a biomarker for predicting the prognosis of gastric cancer.
UMLF-COVID: an unsupervised meta-learning model specifically designed to identify X-ray images of COVID-19 patients
Authors: Miao Rui , Dong Xin , Xie Sheng-Li , Liang Yong , Lo Sio-Long
Published: November 2021

Background

With the rapid spread of COVID-19 worldwide, quick screening for possible COVID-19 patients has become the focus of international researchers. Recently, many deep learning-based Computed Tomography (CT) image/X-ray image fast screening models for potential COVID-19 patients have been proposed. However, the existing models still have two main problems. First, most of the existing supervised models are based on pre-trained model parameters. The pre-training model needs to be constructed on a dataset with features similar to those in COVID-19 X-ray images, which limits the construction and use of the model. Second, the number of categories based on the X-ray dataset of COVID-19 and other pneumonia patients is usually imbalanced. In addition, the quality is difficult to distinguish, leading to non-ideal results with the existing model in the multi-class classification COVID-19 recognition task. Moreover, no researchers have proposed a COVID-19 X-ray image learning model based on unsupervised meta-learning.

Methods

This paper first constructed an unsupervised meta-learning model for fast screening of COVID-19 patients (UMLF-COVID). This model does not require a pre-trained model, which solves the limitation problem of model construction, and the proposed unsupervised meta-learning framework solves the problem of sample imbalance and sample quality.

Results

The UMLF-COVID model is tested on two real datasets, each of which builds a three-category and four-category model. And the experimental results show that the accuracy of the UMLF-COVID model is 3–10% higher than that of the existing models.

Conclusion

In summary, we believe that the UMLF-COVID model is a good complement to COVID-19 X-ray fast screening models.