bluuebunny/crossref_metadata_2025 huggingface dataset:
Atrial fibrillation prediction by combining ECG markers and CMR radiomics
Authors: Pujadas Esmeralda Ruiz , Raisi-Estabragh Zahra , Szabo Liliana , Morcillo Cristian Izquierdo , Campello Víctor M. , Martin-Isla Carlos , Vago Hajnalka , Merkely Bela , Harvey Nicholas C. , Petersen Steffen E. , Lekadir Karim
Published: November 2022

Atrial fibrillation (AF) is the most common cardiac arrhythmia. It is associated with a higher risk of important adverse health outcomes such as stroke and death. AF is linked to distinct electro-anatomic alterations. The main tool for AF diagnosis is the Electrocardiogram (ECG). However, an ECG recorded at a single time point may not detect individuals with paroxysmal AF. In this study, we developed machine learning models for discrimination of prevalent AF using a combination of image-derived radiomics phenotypes and ECG features. Thus, we characterize the phenotypes of prevalent AF in terms of ECG and imaging alterations. Moreover, we explore sex-differential remodelling by building sex-specific models. Our integrative model including radiomics and ECG together resulted in a better performance than ECG alone, particularly in women. ECG had a lower performance in women than men (AUC: 0.77 vs 0.88, p < 0.05) but adding radiomics features, the accuracy of the model was able to improve significantly. The sensitivity also increased considerably in women by adding the radiomics (0.68 vs 0.79, p < 0.05) having a higher detection of AF events. Our findings provide novel insights into AF-related electro-anatomic remodelling and its variations by sex. The integrative radiomics-ECG model also presents a potential novel approach for earlier detection of AF.
Gustatory function of sensilla chaetica on the labial palps and antennae of three tortricid moths (Lepidoptera: Tortricidae)
Authors: Amat Carles , Marion-Poll Frédéric , Navarro-Roldán Miguel A. , Gemeno César
Published: November 2022

In adult Lepidoptera the labial palps are best known for their role in CO2detection, but they can also bear sensilla chaetica which function is unknown. The number and distribution of sensilla chaetica in labial palps was studied using a bright field microscope. To determine if these sensilla have a gustatory function, we performed single sensillum electrophysiology recordings from palp and antennal sensilla of adult moths ofCydia pomonella(L.),Grapholita molesta(Busck) andLobesia botrana(Denis and Shieffermüller). Each sensillum was stimulated with 3 doses of one of four test stimulus (sucrose, fructose, KCl and NaCl). Overall, responses (spikes/s−1) increased with dose, and were higher in the palps than in the antennae, and higher to sugars than to salts. With sugars the response increased with concentration in the palp but not in the antenna. With salts there was a drop in response at the intermediate concentration. The number and position of sensilla chaetica on labial palps was variable among individuals. Sensilla were located in the most exposed areas of the palp. Differences in sensilla distribution were detected between species. Such differences among species and between palps and antenna suggest that taste sensilla on the palps have an unforeseen role in adaptation.
New insights on the active degassing system of the Lipari–Vulcano complex (South Italy) inferred from Local Earthquake Tomography
Authors: Totaro C. , Aloisi M. , Ferlito C. , Orecchio B. , Presti D. , Scolaro S.
Published: November 2022

Seismic tomography is a very powerful and effective approach to look at depths beneath volcanic systems thus helping to better understand their behaviour. The P-wave and S-wave velocity ratio, in particular, is a key parameter useful to discriminate the presence of gas, fluids and melts. We computed the first 3-D overall model of Vp, Vs and Vp/Vs for the Lipari–Vulcano complex, central sector of the Aeolian volcanic archipelago (southern Italy). The investigated area has been characterized in recent times by fumaroles, hydrothermal activity and active degassing. In particular, in the Vulcano Island, several episodes of anomalous increases of fumarole temperature and strong degassing have been recorded in the past decades and the last “crisis”, started in September 2021, is still ongoing. For tomographic inversion we collected ~ 4400 crustal earthquakes that occurred in the last thirty years and we used the LOcal TOmography Software LOTOS. The results clearly depicted two low Vp and Vp/Vs anomalies located up to ~ 8 km depths below Vulcano and the western offshore of Lipari, respectively. These anomalies can be associated to the large presence of gas and they furnish a first picture of the gas-filled volumes feeding the main degassing activity of the area.
Sperm tendency to agglutinate in motile bundles in relation to sperm competition and fertility duration in chickens
Authors: Sayed M. A. M. , Abd-Elhafeez Hanan H. , Afifi O. S. , Marzouk M. W. , El-Sherry Taymour M.
Published: November 2022

A unique sperm behavior was observed in Egyptian chickens. Sperm showed a tendency to agglutinate forming motile thread-like bundles. Sperm agglutination behavior, kinematics, and some morphometric measures were studied in relation to sperm competition and fertility duration in Sharkasi and Dandarawi chickens. Sperm tendency to agglutinate was assessed by examining sperm morphology using scanning electron microscopy, Acridine orange-stained semen smears using fluorescence microscopy, and recording videos of sperm under phase contrast microscope. Sperm velocity and morphometric measures were evaluated using image-J software. To assess sperm competition, Sharkasi and Dandarawi hens were artificially inseminated by semen pools possessing equal number of Sharaksi and Dandarawi sperm. Artificial insemination was repeated ten times. The eggs obtained were incubated, and the hatchlings were discriminated as descending from Sharkasi or Dandarawi fathers according to their phenotype. To assess the fertility duration, Sharkasi and Dandarawi hens were inseminated by semen collected from roosters of the same strain. Eggs were collected for a period of 28 days post-insemination and incubated. Sharkasi spermatozoa showed higher tendency to agglutinate forming longer and thicker motile bundles. No significant differences were observed in sperm curvilinear and straight line velocity and in sperm morphometric measures between Sharkasi and Dandarawi chickens. Sharkasi roosters fathered 81.6% and 67.7% of the hatchlings produced by Sharkasi and Dandarawi mothers, respectively. The fertility period in Sharkasi and Dandarawi was 22 and 14 days, respectively. We suggest that the differences seen in sperm competitiveness and fertility duration can be attributed to sperm agglutination behavior.
Factors influencing lion movements and habitat use in the western Serengeti ecosystem, Tanzania
Authors: Schooler Sarah L. , Finnegan Shannon P. , Fowler Nicholas L. , Kellner Kenneth F. , Lutto Ashley L. , Parchizadeh Jamshid , van den Bosch Merijn , Zubiria Perez Alejandra , Masinde Lusato M. , Mwampeta Stanslaus B. , Boone Hailey M. , Gantchoff Mariela G. , Hill Jacob E. , Kautz Todd M. , Wehr Nathaniel H. , Fyumagwa Robert , Belant Jerrold L.
Published: November 2022

Protected areas that restrict human activities can enhance wildlife habitat quality. Efficacy of protected areas can be improved with increased protection from illegal activities and presence of buffer protected areas that surround a core protected area. Habitat value of protected areas also can be affected by seasonal variation in anthropogenic pressures. We examined seasonal space use by African lions (Panthera leo) within a core protected area, Serengeti National Park, Tanzania, and surrounding buffer protected areas with varying protection strengths. We used lion locations in logistic regression models during wet and dry seasons to estimate probability of use in relation to protection strength, distance to protected area edge, human and livestock density, distance to roads and rivers, and land cover. Lions used strongly protected buffer areas over the core protected area and unprotected areas, and moved away from protected area boundaries toward the core protected area when buffer protected areas had less protection. Lions avoided high livestock density in the wet season and high human density in the dry season. Increased strength of protection can decrease edge effects on buffer areas and help maintain habitat quality of core protected areas for lions and other wildlife species.
Wave-like patterns in parameter space interpreted as evidence for macroscopic effects resulting from quantum or quantum-like processes in the brain
Authors: Kurtev Stoyan
Published: November 2022

Data from eight numerosity estimation experiments reliably exhibit wave-like patterns in plots of the standard deviations of the response times along the abstract parameter of the magnitude of the error in the numerosity estimation. An explanation for this phenomenon is proposed in terms of an analogy between response times and error magnitude on one hand, and energy and position of quantum particles on the other, constructed using an argument for an overlap between the mathematical apparatus describing Hopfield-type neural networks and quantum systems, established by some researchers. Alternative explanations are presented within the traditional explanatory framework of oscillations due to neural firing, involving hypothetical mechanisms for converting oscillation patterns in time to oscillation patterns in the space of an abstract parameter, such as the magnitude of the error during numerosity estimation. The viability of the proposal of causal influences propagating from the microscale of quantum phenomena to the macroscale of human behavior, needed for the first type of explanation, is exemplified by the phenomenon of magnetoreception in some species of birds, which is allegedly quantum in nature.
Sharp dose profiles for high precision proton therapy using strongly focused proton beams
Authors: Reaz Fardous , Sjobak Kyrre Ness , Malinen Eirik , Edin Nina Frederike Jeppesen , Adli Erik
Published: November 2022

The main objective of radiotherapy is to exploit the curative potential of ionizing radiation while inflicting minimal radiation-induced damage to healthy tissue and sensitive organs. Proton beam therapy has been developed to irradiate the tumor with higher precision and dose conformity compared to conventional X-ray irradiation. The dose conformity of this treatment modality may be further improved if narrower proton beams are used. Still, this is limited by multiple Coulomb scattering of protons through tissue. The primary aim of this work was to develop techniques to produce narrow proton beams and investigate the resulting dose profiles. We introduced and assessed three different proton beam shaping techniques: (1) metal collimators (100/150 MeV), (2) focusing of conventional- (100/150 MeV), and (3) focusing of high-energy (350 MeV, shoot-through) proton beams. Focusing was governed by the initial value of the Twiss parameter$$\alpha$$α ($$\alpha _0$$α0), and can be implemented with magnetic particle accelerator optics. The dose distributions in water were calculated by Monte Carlo simulations using Geant4, and evaluated by target to surface dose ratio (TSDR) in addition to the transverse beam size ($$\sigma _T$$σT) at the target. The target was defined as the location of the Bragg peak or the focal point. The different techniques showed greatly differing dose profiles, where focusing gave pronouncedly higher relative target dose and efficient use of primary protons. Metal collimators with radii$$<\,2~\mathrm{mm}$$<2mmgave low TSDRs ($$<~0.7$$<0.7) and large$$\sigma _T$$σT($$>~3.6~\hbox {mm}$$>3.6mm). In contrast, a focused beam of conventional ($$150~\hbox {MeV}$$150MeV) energy produced a very high TSDR ($$>~80$$>80) with similar$$\sigma _T$$σTas a collimated beam. High-energy focused beams were able to produce TSDRs$$>~100$$>100and$$\sigma _T$$σTaround 1.5 mm. From this study, it appears very attractive to implement magnetically focused proton beams in radiotherapy of small lesions or tumors in close vicinity to healthy organs at risk. This can also lead to a paradigm change in spatially fractionated radiotherapy. Magnetic focusing would facilitate FLASH irradiation due to low losses of primary protons.
Female bone physiology resilience in a past Polynesian Outlier community
Authors: Miszkiewicz Justyna J. , Buckley Hallie R. , Feldman Michal , Kiko Lawrence , Carlhoff Selina , Naegele Kathrin , Bertolini Emilie , Guimarães Nathalia R. Dias , Walker Meg M. , Powell Adam , Posth Cosimo , Kinaston Rebecca L.
Published: November 2022

Remodelling is a fundamental biological process involved in the maintenance of bone physiology and function. We know that a range of health and lifestyle factors can impact this process in living and past societies, but there is a notable gap in bone remodelling data for populations from the Pacific Islands. We conducted the first examination of femoral cortical histology in 69 individuals from ca. 440–150 BP Taumako in Solomon Islands, a remote ‘Polynesian Outlier’ island in Melanesia. We tested whether bone remodelling indicators differed between age groups, and biological sex validated using ancient DNA. Bone vascular canal and osteon size, vascular porosity, and localised osteon densities, corrected by femoral robusticity indices were examined. Females had statistically significantly higher vascular porosities when compared to males, but osteon densities and ratios of canal-osteon (~ 8%) did not differ between the sexes. Our results indicate that, compared to males, localised femoral bone tissue of the Taumako females did not drastically decline with age, contrary to what is often observed in modern populations. However, our results match findings in other archaeological samples—a testament to past female bone physiology resilience, also now observed in the Pacific region.
Membrane damage mechanism of protocatechualdehyde against Micrococcus luteus and its effect on pork quality characteristics
Authors: Liao Sichen , Gong Guoli , Wang Xuyang , Tian Lu
Published: November 2022

This study investigated the mechanism of membrane damage by protocatechualdehyde (PCA) against Micrococcus luteus and assessed effects of PCA on the sensory and physicochemical properties of pork. The mechanism of PCA inhibition on M. luteus was studied by determining the minimum inhibitory concentration (MIC) based on membrane potential, intracellular ATP concentration, intracellular pH, confocal laser scanning microscopy (CLSM), and field emission gun scanning electron microscopy (FEG-SEM). The results showed that the MIC of PCA against M. luteus was 1.25 mg/mL. Hyperpolarization of the bacterial cell membrane, a decrease in the intracellular ATP concentration, and intracellular pH indicated that PCA damaged the cell membrane of M. luteus. FEG-SEM observation revealed that PCA could cause surface collapse, cell membrane rupture, and content outflow of M. luteus. Additionally, PCA was found to inhibit increases in the total number of colonies, the thiobarbituric acid reactive substances (TBARS) value growth rate, and moisture mobility in raw pork. Additionally, it improved the color and texture of raw pork, all of which effectively prolonged its shelf life. This study will encourage the application of PCA as a natural antibacterial agent in the food industry.
Correlates of domain-specific sedentary behaviors and objectively assessed sedentary time among elementary school children
Authors: Koohsari Mohammad Javad , Oka Koichiro , Shibata Ai , McCormack Gavin R. , Hanibuchi Tomoya , Nakaya Tomoki , Ishii Kaori
Published: November 2022

Understanding the correlates of sedentary behavior among children is essential in developing effective interventions to reduce sitting time in this vulnerable population. This study aimed to identify correlates of domain-specific sedentary behaviors and objectively assessed sedentary time among a sample of children in Japan. Data from 343 children (aged 6–12 years) living in Japan were used. Domain-specific sedentary behaviors were assessed using a questionnaire. Total sedentary time was estimated using hip-worn accelerometers. Twenty-two potential correlates across five categories (parental characteristics, household indoor environment, residential neighborhood environment, school environment, and school neighborhood environment) were included. Multivariable linear regression models were used to identify correlates of domain-specific sedentary behaviors and objectively assessed sedentary time. Eight correlates were significantly associated with children’s domain-specific sedentary behaviors: mother’s and father’s age, mother’s educational level, having a video/DVD recorder/player, having a video console, having a TV one’s own room, home’s Walk Score®, and pedestrian/cycling safety. No significant associations were found between potential correlates and accelerometer-based total sedentary time. These findings highlight that strategies to reduce children’s sedentary time should consider the context of these behaviors. For example, urban design attributes such as perceived pedestrian and cycling safety can be improved to reduce children’s car sitting time.
Microbiological analysis and bioremediation bioassay for characterization of industrial effluent
Authors: Zabermawi Nidal Mohammed Omar , Alyhaiby Amani H. , El-Bestawy Ebtesam A.
Published: November 2022

This study aims to investigate bacteria for biodegradation of oil pollutants from oily industrial wastewater to be used as bioremediation tools and to determine the characterization of bioremediation bioassays. A screening bioassay was carried out using six exogenous environmental bacterial strains to degrade oily pollution, which indicated promising clearance of the oily wastewater. Two strains, namely Enterobacter cloacae 279-56 (R4) and Pseudomonas otitis MCC10330 (R19), could successfully eliminate oil content and reasonable removal of the organic load. Results showed that the two promising bacterial candidates (R4 and R19) were selected according to the preliminary screening of the six tested bacteria considered the most efficient for all the tested parameters. The highest Removal Efficiency (Removal Efficiency resulted in Residual levels of total dissolved solids (TDS), biochemical oxygen demand, chemical oxygen demand, and Oil content in the treated oily wastewater effluents are 1940, 171, 131, and 84 mg/l respectively where these results are not within safe discharge limits, except for TDS. Hence, the bioremediation assays were carried out using the mixed culture since it was the most efficient strain for degrading all tested parameters.
Monensin inhibits mast cell mediated airway contractions in human and guinea pig asthma models
Authors: Liu Jielu , Nie Mu , Dong Caijuan , Säfholm Jesper , Pejler Gunnar , Nilsson Gunnar , Adner Mikael
Published: November 2022

Asthma is a common respiratory disease associated with airway hyperresponsiveness (AHR), airway inflammation and mast cell (MC) accumulation in the lung. Monensin, an ionophoric antibiotic, has been shown to induce apoptosis of human MCs. The aim of this study was to define the effect of monensin on MC responses, e.g., antigen induced bronchoconstriction, and on asthmatic features in models of allergic asthma. Tracheal segments from house dust mite (HDM) extract sensitized guinea pigs were isolated and exposed to monensin, followed by histological staining to quantify MCs. Both guinea pig tracheal and human bronchi were used for pharmacological studies in tissue bath systems to investigate the monensin effect on tissue viability and antigen induced bronchoconstriction. Further, an HDM-induced guinea pig asthma model was utilized to investigate the effect of monensin on AHR and airway inflammation. Monensin decreased MC number, caused MC death, and blocked the HDM or anti-IgE induced bronchoconstriction in guinea pig and human airways. In the guinea pig asthma model, HDM-induced AHR, airway inflammation and MC hyperplasia could be inhibited by repeated administration of monensin. This study indicates that monensin is an effective tool to reduce MC number and MCs are crucial for the development of asthma-like features.
Characterization of bacterial diversity between two coastal regions with heterogeneous soil texture
Authors: Zakavi Maryam , Askari Hossein , Shahrooei Mohammad
Published: November 2022

Studying microbial diversity and the effects of external factors on the microbiome could expand our understanding of environmental alterations. Silt and sand are mineral particles that form soil texture and even though most of the soils on earth contain a fraction of them and some other soils form almost by them, their effects on the microbiome remained to elucidate. In this study, the bacterial biodiversity of sand and silt clay soils was investigated. Furthermore, their effects on plant growth have been determined. Our data showed that biodiversity and biomass of microbiome are higher in silt-based soil. It is interesting that the pseudomonas genera only exist in silt-based soil while it is in the absence of sand-based soil. In contrast, B. thuringiensis could be found in sand-based soil while it is not found in silt texture. Our data also demonstrated that there are no significant changes in stress response between the two groups however, differential physiological changes in plants inoculated with silt and sand based bacterial isolates have been observed. This data could indicate that smaller size particles could contain more bacteria with higher biodiversity due to providing more surfaces for bacteria to grow.
A bioinformatics approach to the identification of novel deleterious mutations of human TPMT through validated screening and molecular dynamics
Authors: Saxena Sidharth , Krishna Murthy T. P. , Chandrashekhar C. R. , Patil Lavan S. , Aditya Abhinav , Shukla Rohit , Yadav Arvind Kumar , Singh Tiratha Raj , Samantaray Mahesh , Ramaswamy Amutha
Published: November 2022

Polymorphisms of Thiopurine S-methyltransferase (TPMT) are known to be associated with leukemia, inflammatory bowel diseases, and more. The objective of the present study was to identify novel deleterious missense SNPs of TPMT through a comprehensive in silico protocol. The initial SNP screening protocol used to identify deleterious SNPs from the pool of all TPMT SNPs in the dbSNP database yielded an accuracy of 83.33% in identifying extremely dangerous variants. Five novel deleterious missense SNPs (W33G, W78R, V89E, W150G, and L182P) of TPMT were identified through the aforementioned screening protocol. These 5 SNPs were then subjected to conservation analysis, interaction analysis, oncogenic and phenotypic analysis, structural analysis, PTM analysis, and molecular dynamics simulations (MDS) analysis to further assess and analyze their deleterious nature. Oncogenic analysis revealed that all five SNPs are oncogenic. MDS analysis revealed that all SNPs are deleterious due to the alterations they cause in the binding energy of the wild-type protein. Plasticity-induced instability caused by most of the mutations as indicated by the MDS results has been hypothesized to be the reason for this alteration. While in vivo or in vitro protocols are more conclusive, they are often more challenging and expensive. Hence, future research endeavors targeted at TPMT polymorphisms and/or their consequences in relevant disease progressions or treatments, through in vitro or in vivo means can give a higher priority to these SNPs rather than considering the massive pool of all SNPs of TPMT.
Learned end-to-end high-resolution lensless fiber imaging towards real-time cancer diagnosis
Authors: Wu Jiachen , Wang Tijue , Uckermann Ortrud , Galli Roberta , Schackert Gabriele , Cao Liangcai , Czarske Juergen , Kuschmierz Robert
Published: November 2022

Recent advances in label-free histology promise a new era for real-time diagnosis in neurosurgery. Deep learning using autofluorescence is promising for tumor classification without histochemical staining process. The high image resolution and minimally invasive diagnostics with negligible tissue damage is of great importance. The state of the art is raster scanning endoscopes, but the distal lens optics limits the size. Lensless fiber bundle endoscopy offers both small diameters of a few 100 microns and the suitability as single-use probes, which is beneficial in sterilization. The problem is the inherent honeycomb artifacts of coherent fiber bundles (CFB). For the first time, we demonstrate an end-to-end lensless fiber imaging with exploiting the near-field. The framework includes resolution enhancement and classification networks that use single-shot CFB images to provide both high-resolution imaging and tumor diagnosis. The well-trained resolution enhancement network not only recovers high-resolution features beyond the physical limitations of CFB, but also helps improving tumor recognition rate. Especially for glioblastoma, the resolution enhancement network helps increasing the classification accuracy from 90.8 to 95.6%. The novel technique enables histological real-time imaging with lensless fiber endoscopy and is promising for a quick and minimally invasive intraoperative treatment and cancer diagnosis in neurosurgery.
Photonic Weyl semimetals in pseudochiral metamaterials
Authors: Chern Ruey-Lin , Chou Yi-Ju
Published: November 2022

We investigate the photonic topological phases in pseudochiral metamaterials characterized by the magnetoelectric tensors with symmetric off-diagonal chirality components. The underlying medium is considered a photonic analogue of the type-II Weyl semimetal featured with two pairs of tilted Weyl cones in the frequency-wave vector space. As the ’spin’-degenerate condition is satisfied, the photonic system consists of two hybrid modes that are completely decoupled. By introducing the pseudospin states as the basis for the hybrid modes, the photonic system is described by two subsystems in terms of the spin-orbit Hamiltonians with spin 1, which result in nonzero spin Chern numbers that determine the topological properties. Surface modes at the interface between vacuum and the pseudochiral metamaterial exist in their common gap in the wave vector space, which are analytically formulated by algebraic equations. In particular, the surface modes are tangent to both the vacuum light cone and the Weyl cones, which form two pairs of crossing surface sheets that are symmetric about the transverse axes. At the Weyl frequency, the surface modes that connect the Weyl points form four Fermi arc-like states as line segments. Topological features of the pseudochiral metamaterials are further illustrated with the robust transport of surface modes at an irregular boundary.
Real-world experience of intravenous ferric derisomaltose evaluated through safety and efficacy reporting in the UK
Authors: Sinclair Rhona C. F. , Nadaraja Sean , Kennedy Nicholas A. , Wakatsuki Mai , Bhandari Sunil
Published: November 2022

Ferric derisomaltose (FDI; Monofer) is used in clinical practice to treat iron deficiency, but the safety and efficacy of FDI has not been robustly evaluated in a large real-world study. This retrospective, multicentre, audit-based, observational study provides pragmatic information about safety and clinical responses with FDI across therapy areas and patient populations, helping to facilitate treatment decisions. Participating sites provided data from the medical records of adults who had received ≥ 1 FDI infusion. The primary outcome was the incidence of adverse reactions within 24 hours of the FDI infusion. Secondary outcomes included the change from baseline in haemoglobin and ferritin up to 12 months post infusion. In total, 19 sites provided data for a total of 7354 FDI-treated patients; 64.3% of patients were female, and 42.2% were aged ≥ 70 years. Surgery was the main hospital specialty (34.5%). The incidence of any recorded adverse reactions, hypersensitivity reactions, and anaphylaxis were 1.7%, 0.4%, and < 0.1%, respectively, regardless of baseline anaemia status. Statistically significant increases in haemoglobin and ferritin were observed between baseline and Month 4 following FDI treatment (p < 0.0001). Improvements in haemoglobin were more pronounced for hospital specialties where operative blood loss is expected (surgery/obstetrics) compared with those where blood loss is not expected. This study provides real-world clinical evidence for the low risk of adverse reactions with FDI across diverse patient populations, providing reassurance that intravenous iron is not associated with serious toxicity. These findings may inform changes in intravenous iron delivery to provide effective therapy to more patients in need.
Development of an intrinsic health risk prediction model for camera-based monitoring of older adults living alone
Authors: Kim Minji , Hong Song-iee , Youm Sekyoung
Published: November 2022

The number of older adults in Korea is increasing, along with the number of depressed older patients. The causes of depression in older adults include social isolation with negligible interaction with others, irregular nutritional habits, and self-negligence, i.e., they do not engage in any activity. These factors, self-negligence, social isolation, and irregular nutritional habits, are defined as inherent health risks, and in this study, we detected them. These factors can only be derived through long-term monitoring, but the current monitoring system for older adults is severely limited as it focuses only on emergencies, such as “falls.” Therefore, in this study, the goal was to perform long-term monitoring using a camera. In order to capture the physical characteristics of the older adults, the ETRI-Activity3D data were used for training, and the skeleton-based action recognition algorithm Posec3d was used. By defining 90 frames as the time taken for one action, we built a monitoring system to enable long-term monitoring of older adult by performing multiple action detection in one video. A reliable monitoring system, with 98% accuracy, 98% precision, 99% recall, and 98% F1, was successfully established for health monitoring of older adults. This older adult monitoring technology is expected to improve the quality of medical services in a medical environment as well as the objective, activities of daily living test, which does not depend on the observer through daily life detection.
A formula to improve the reliability of optical axial length measurement in IOL power calculation
Authors: De Bernardo Maddalena , Cione Ferdinando , Capasso Luigi , Coppola Alessia , Rosa Nicola
Published: November 2022

To verify the influence of axial length (AL) variations after cataract surgery in IOL power calculation. Patients underwent ophthalmic evaluation before surgery, including optical biometry with IOLMaster 500. Same exams were repeated 2 months after surgery: AL of operated eye was evaluated using two modes (pseudophakic/aphakic options). Mean Keratometry and AL changes were analyzed. Furthermore, refractive prediction error (PE) was back-calculated with Barrett Universal-II, Hoffer-Q, Holladay-1 and SRK/T formulas. To eliminate any systematic error, the mean error (ME) was zeroed-out for each formula. MEs and median absolute errors (MedAEs) of PEs were analyzed. Two-hundred-one operated eyes of 201 patients and 201 opposite eyes were evaluated. In operated eyes, mean AL difference was − 0.11 ± 0.07 mm (p < 0.001) with pseudophakic option and 0.00 ± 0.07 mm (p = 0.922) with aphakic option. There were not-statistically significant differences between MedAE of PEs calculated after zeroing-out the ME with different ALs (p > 0.05). Instead, only MEs of PEs obtained with postoperative ALs-pseudophakic option were not-statistically different from zero (p > 0.05). AL measurement change after cataract surgery is probably due to a systematic error in optical biometer in case of phakic eyes. A correction factor applied to preoperative AL could eliminate any systematic error in IOL power calculation without modifying the lens constant.
A novel classification of HCC basing on fatty-acid-associated lncRNA
Authors: Xu Yating , Yu Xiao , Zhang Qiyao , He Yuting , Guo Wenzhi
Published: November 2022

Aberrant long noncoding RNA (lncRNA) expression and fatty acid signaling dysfunction both contribute to hepatocellular carcinoma (HCC) occurrence and development. However, the relationship and interaction mechanism between lncRNAs and fatty acid signaling in HCC remain unclear. Data regarding RNA expression and clinical outcomes for patients with HCC were obtained from The Cancer Genome Atlas (TCGA), HCCDB, and the Gene Expression Omnibus (GEO) databases. Hallmark pathways were identified using the single-sample gene set enrichment analysis (ssGSEA) method. ConsensusClusterPlus was used to establish a consistency matrix for classifying samples into three subtypes. A risk signature was established, and predictive values for key lncRNAs related to prognosis were evaluated using Kaplan–Meier analysis and receiver operating characteristic curves. The ESTIMATE algorithm, MCP-Counter, and ssGSEA were used to evaluate the characteristics of the tumor immune microenvironment. The CTRP2.0 and PRISM were used to analyze drug sensitivity in HCC subtypes. We discovered seven fatty-acid-associated lncRNAs with predictive prognostic capabilities, including TRAF3IP2-AS1, SNHG10, AL157392.2, LINC02641, AL357079.1, AC046134.2, and A1BG-AS. Three subtypes were obtained, which presented with differences in prognosis, clinical information, mutation features, pathway traits, immune characteristics, and drug sensitivity. The seven key lncRNAs identified in this study might serve as promising biomarkers for predicting prognosis in patients with HCC, and the three HCC subtypes classified according to lncRNA expression profiles could improve HCC classification.
Treatment burden among patients with heart failure attending cardiac clinic of Tikur Anbessa Specialized Hospital: an explanatory sequential mixed methods study
Authors: Hassen Minimize , Mekonnen Desalew , Muhammed Oumer Sada
Published: November 2022

Emerging evidences hypothesized that patients with heart failure are susceptible to experience treatment burden. Despite this fact, no attempt was made so far to address this neoteric construct in the sub-Saharan African health care context. Hence, this study aimed to assess patients’ and health care providers’ perspectives on how to decrease treatment burden among patients with heart failure attending the adult cardiac clinic of Tikur Anbessa Specialized Hospital (TASH). An explanatory sequential mixed methods study was conducted at the adult cardiac clinic of TASH, Addis Ababa, Ethiopia from August 01 to September 30, 2021. Simple random and purposive sampling techniques were employed to select participants for quantitative and qualitative studies, respectively. Descriptive analysis was done to summarize the quantitative data. Logistic regression analysis was performed to identify predictors of treatment burden. P value < 0.05 was considered to declare statistical significance. Qualitative data were analyzed by using thematic analysis. A total of 325 patients were enrolled in the quantitative study. For the qualitative study, 14 patients and 11 health care providers (five nurses and six medical doctors) were included. Participants mean global Treatment Burden Questionnaire (TBQ-15) score was 27.22 ± 19.35. Approximately 12% (n = 38) patients indicated high treatment burden (TBQ-15 global score ≥ 59) with a median global score of 63(60–69). Higher education level (adjusted odds ratio [AOR] = 6.66, 95% confidence interval [CI]: 1.16–38.43), presence of two and more comorbidities (AOR = 2.74, 95%CI: 1.02–7.39), daily intake of more than five pills (AOR = 7.38, 95%CI: 2.23–24.41), poor medication availability (AOR = 3.33, 95%CI: 1.33–8.36), presence of medication adverse effects (AOR = 4.04, 95%CI: 1.63–10.03), and higher monthly cost of medication (AOR = 5.29, 95%CI: 1.46–19.18) were predictors of treatment burden. Patients and healthcare providers' propositions were primarily focused on improving self-care management, structural organization of the clinic and hospital, and healthcare system provision. Our findings demonstrated that a substantial proportion of patients faced low levels of treatment burden. This study unveiled that improving self-care management, structural organization of the clinic, and healthcare system provision had paramount importance to reducing treatment burden. Hence, factors affecting treatment burden should be considered when designing tailored healthcare interventions for patients with heart failure.
Solar water disinfection in large-volume containers: from the laboratory to the field. A case study in Tigray, Ethiopia
Authors: García-Gil Ángela , García-Muñoz Rafael A. , Martínez-García Azahara , Polo-López Maria Inmaculada , Wasihun Araya Gebreyesus , Teferi Mekonen , Asmelash Tsehaye , Conroy Ronan , McGuigan Kevin G. , Marugán Javier
Published: November 2022

The lack of safe drinking water affects communities in low-to-medium-income countries most. This barrier can be overcome by using sustainable point-of-use water treatments. Solar energy has been used to disinfect water for decades, and several efforts have been made to optimise the standard procedure of solar water disinfection (SODIS process). However, the Health Impact Assessment of implementing advanced technologies in the field is also a critical step in evaluating the success of the optimisation. This work reports a sustainable scaling-up of SODIS from standard 2 L bottles to 25 L transparent jerrycans (TJC) and a 12-month field implementation in four sites of Tigray in Ethiopia, where 80.5% of the population lives without reliable access to safe drinking water and whose initial baseline average rate of diarrhoeal disease in children under 5 years was 13.5%. The UVA dose required for 3-log reduction of E. coli was always lower than the minimum UVA daily dose received in Tigray (9411 ± 55 Wh/m2). Results confirmed a similar decrease in cases of diarrhoea in children in the implementation (25 L PET TJC) and control (2 L PET bottles) groups, supporting the feasibility of increasing the volume of the SODIS water containers to produce safer drinking water with a sustainable and user-friendly process.
MiR-27a as a diagnostic biomarker and potential therapeutic target in systemic sclerosis
Authors: Bayati Paria , Kalantari Mahsa , Assarehzadegan Mohammad-Ali , Poormoghim Hadi , Mojtabavi Nazanin
Published: November 2022

Systemic sclerosis (SSc) or scleroderma is a multiorgan rheumatoid disease characterized by skin tightening or organ dysfunction due to fibrosis, vascular damage, and autoimmunity. No specific cause has been discovered for this illness, and hence no effective treatment exists for it. On the other hand, due to the lack of diagnostic biomarkers capable of effectively and specifically differentiating the patients, early diagnosis has not been possible. Due to their potent regulatory roles in molecular pathways, microRNAs are among the novel candidates for the diagnosis and treatment of diseases like SSc. MiR-27a is a microRNA known for its role in the pathogenesis of fibrosis and cancer, both of which employ similar signaling pathways; hence we hypothesized that Mir-27a could be dysregulated in the blood of individuals affected by SSc and it might be useful in the diagnosis or treatment of this disease. Blood was collected from 60 SSc patients (30 limited and 30 diffuse) diagnosed by a rheumatologist according to ACR/AULAR criteria; following RNA isolation and cDNA synthesis; real-time qPCR was performed on the samples using Taq-Man probes and data were analyzed by the ΔΔCT method. Also, potential targets of miR-27a were evaluated using bioinformatics. It was revealed that miR-27a was significantly down-regulated in SSc patients in comparison to healthy individuals, but there was no difference in miR-27 expression between limited and diffused SSc patients. Besides, miR-27a was found to target several contributing factors to SSc. It seems that miR-27a has a protective role in SSc, and its downregulation could result in the disease's onset. Based on bioinformatics analyses, it is speculated that miR-27a likely targets factors contributing to the pathogenesis of SSc, which are elevated upon the downregulation of miR-27a; hence, miR-27a mimics could be considered as potential therapeutic agents for the treatment of SSc in future studies. Since no difference was observed between limited and diffuse patient groups, it is unlikely that this microRNA has a role in disease progression. According to ROC analysis of qPCR data, miR-27a could be employed as a valuable diagnostic biomarker for SSc.
Classification of flood-generating processes in Africa
Authors: Tramblay Yves , Villarini Gabriele , Saidi Mohamed Elmehdi , Massari Christian , Stein Lina
Published: November 2022

River flooding has large societal and economic impacts across Africa. Despite the importance of this topic, little is known about the main flood generating mechanisms in Africa. This study is based on 13,815 flood events that occurred between 1981 and 2018 in 529 catchments. These flood events are classified to identify the different flood drivers: excess rains, long rains and short rains. Out of them, excess rains on saturated soils in Western Africa, and long rains for catchments in Northern and Southern Africa, are the two dominant mechanisms, contributing to more than 75% of all flood events. The aridity index is strongly related to the spatial repartition of the different flood generating processes showing the climatic controls on floods. Few significant changes were detected in the relative importance of these drivers over time, but the rather short time series available prevent a robust assessment of flood driver changes in most catchments. The major implication of these results is to underline the importance of soil moisture dynamics, in addition to rainfall, to analyze the evolution of flood hazards in Africa.
Shifting in the global flood timing
Authors: Fang Gonghuan , Yang Jing , Li Zhi , Chen Yaning , Duan Weili , Amory Charles , De Maeyer Philippe
Published: November 2022

Climate change will have an impact on not only flood magnitude but also on flood timing. This paper studies the shifting in flood timing at 6167 gauging stations from 1970 to 2010, globally. The shift in flood timing and its relationship with three influential factors (maximum 7-day precipitation, soil moisture excess, and snowmelt) are investigated. There is a clear global pattern in the mean flooding date: winter (Dec–Feb) across the western Coastal America, western Europe and the Mediterranean region, summer (Jun–Aug) in the north America, the Alps, Indian Peninsula, central Asia, Japan, and austral summer (Dec–Feb) in south Africa and north Australia area. The shift in flood timing has a trend from − 22 days per decade (earlier) to 28 days per decade (delayed). Earlier floods were found extensively in the north America, Europe and northeast Australia while delayed floods were prevailing in the Amazon, Cerrado, south Africa, India and Japan. Earlier flood timing in the north America and Europe was caused by earlier snowmelt while delayed extreme soil moisture excess and precipitation have jointly led to delayed floods around the monsoon zone, including south Africa, India and Japan. This study provides an insight on the shifting mechanism of flood timing, and supports decisions on the global flood mitigation and the impact from future climate change.
Sustained productivity and agronomic potential of perennial rice
Authors: Zhang Shilai , Huang Guangfu , Zhang Yujiao , Lv Xiutao , Wan Kejiang , Liang Jian , Feng Yupeng , Dao Jinrong , Wu Shukang , Zhang Lin , Yang Xu , Lian Xiaoping , Huang Liyu , Shao Lin , Zhang Jing , Qin Shiwen , Tao Dayun , Crews Timothy E. , Sacks Erik J. , Lyu Jun , Wade Len J. , Hu Fengyi
Published: November 2022

There is an urgent need for agricultural systems to intensify sustainably, increasing crop productivity, farmer livelihoods and soil health while using fewer resources. Crop perennialization, the conversion of especially annual grains to perennial forms, has shown such possibility. Here we report the successful breeding of perennial rice and assess its performance and potential. Domesticated, annual Asian rice (Oryza sativa) was hybridized with its perennial African relative Oryza longistaminata. From a single planting, irrigated perennial rice produced grain for eight consecutive harvests over four years, averaging 6.8 Mg ha−1 harvest−1 versus the 6.7 Mg of replanted annual rice, which required additional labour and seed. Four years of cropping with perennial rice resulted in soils accumulating 0.95 Mg ha–1 yr–1 organic carbon and 0.11 Mg ha−1 yr−1 nitrogen, along with increases in soil pH (0.3–0.4) and plant-available water capacity (7.2 mm). Perennial cultivars are strongly preferred by farmers; growing them saves 58.1% of labour and 49.2% of input costs in each regrowth cycle. In 2021, perennial rice was grown on 15,333 ha by 44,752 smallholder farmers in southern China. Suited to a broad range of frost-free environments between 40° N and 40° S, perennial rice is a step change with potential to improve livelihoods, enhance soil quality and inspire research on other perennial grains.
Chromosome-level genome and population genomics reveal evolutionary characteristics and conservation status of Chinese indigenous geese
Authors: Ouyang Jing , Zheng Sumei , Huang Min , Tang Hongbo , Qiu Xiaohui , Chen Shoujin , Wang Zhangzhang , Zhou Zhongdong , Gao Yuren , Xiong Yanpeng , Zeng Guohua , Huang Jimin , He Jiugen , Ren Jun , Chen Hao , Yan Xueming
Published: November 2022

Geese are herbivorous birds that play an essential role in the agricultural economy. We construct the chromosome-level genome of a Chinese indigenous goose (the Xingguo gray goose, XGG; Anser cygnoides) and analyze the adaptation of fat storage capacity in the goose liver during the evolution of Anatidae. Genomic resequencing of 994 geese is used to investigate the genetic relationships of geese, which supports the dual origin of geese (Anser cygnoides and Anser anser). Chinese indigenous geese show higher genetic diversity than European geese, and a scientific conservation program can be established to preserve genetic variation for each breed. We also find that a 14-bp insertion in endothelin receptor B subtype 2 (EDNRB2) that determines the white plumage of Chinese domestic geese is a natural mutation, and the linkaged alleles rapidly increase in frequency as a result of genetic hitchhiking, leading to the formation of completely different haplotypes of white geese under strong artificial selection. These genomic resources and our findings will facilitate marker-assisted breeding of geese and provide a foundation for further research on geese genetics and evolution.