bluuebunny/crossref_metadata_2025 huggingface dataset:
Therapeutic effects of acupuncture in rheumatoid arthritis are associated with centromere protein F expression
Authors: Dong Weitao , Wang Bin , Zhang Rongchao , Cao Junyi , Wu Rong
Published: May 2022

Rheumatoid arthritis is a globally common autoimmune inflammatory disease found especially in China. Acupuncture (AP), a traditional Chinese medicine (TCM) treatment method, is commonly used for treating rheumatoid arthritis. Many studies have demonstrated that acupuncture alone or in combination with other treatments is beneficial to treat clinical situation of rheumatoid arthritis, thus improving function and quality of life. In this study, we found that centromere protein F (CENPF) is a key gene in rheumatoid arthritis with acupuncture treatment by using differentially expressed genes (DEGs) and random forest model analysis of GSE57983 and GSE77298. Acupuncture helps to up-regulate the expression of CENPF in tissues in rheumatoid arthritis. Functionally, overexpression of CENPF inhibits monocyte chemoattractant protein (MCP)-1, tumor necrosis factor (TNF)-α, and Interleukin (IL)-6 expressions whereas deficiency of CENPF facilitates MCP-1, TNF-α, and IL-6 expressions in a collagen-induced arthritis (CIA) rat model. Furthermore, knocked down CENPF with acupuncture treatment antagonizes the inhibition of MCP-1, TNF-α, and IL-6 expressions in a CIA rat model. CENPF could be a crucial biomarker in regulating function of acupuncture in treating rheumatoid arthritis.
Objective: The objective of this study is to study the critical role of CENPF in regulation of rheumatoid arthritis with acupuncture treatment.
Methods: PCA was used to analyze the different expression genes between AP treatment group and control group. Volcano plot and random forest model were used to analyze the decreased and increased expression genes. RT-qPCR and IF were used to measure the expression of CENPF in CIA model rat with or without AP treatment. The expression of MCP-1, TNF-α and IL-6 was measured by western blotting. The pathology character and arthritis index were used to analyze the severity of joint injury.
Results: PCA data showed that the expression of genes was different between AP treatment group and control group from GEO datasets. Volcano plot and random forest model analysis indicated that CENPF is the most significantly increased expression gene after AP treatment. RT-qPCR and IF assay showed that CENPF is reduced expression in CIA model rat, while CENPF is upregulated expression in CIA model rat with AP treatment. Furthermore, overexpression of CENPF reduced the increasing of MCP-1, TNF-α and IL-6 in CIA model rat. On the contrary, CENPF deficiency induced the expression of MCP-1, TNF-α and IL-6 in CIA model rat. Additionally, the expression of MCP-1, TNF-α and IL-6 in CIA model rat was suppressed, whereas knockdown of CENPF antagonized the decrease of MCP-1, TNF-α and IL-6 in CIA model rat with AP treatment.
Conclusions: CENPF may be a key gene in regulation of the therapeutic effects of acupuncture in rheumatoid arthritis.
Dexmedetomidine reduces dextran sulfate sodium (DSS)-induced NCM460 cell inflammation and barrier damage by inhibiting RhoA/ROCK signaling pathway
Authors: Han Xin , Wu Yini , Zhuang Yongwei , Zhang Jianmei
Published: May 2022

Objective: This study investigated the role of dexmedetomidine (DEX) in dextran sulfate sodium (DSS)-induced NCM460 cells.
Material and Methods: The viability and apoptosis of NCM460 cells treated with DEX with or without DSS were detected by CCK-8 and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) assay. The level of inflammatory factors and expression of inflammation-related proteins, tight junction proteins and Ras homolog gene family, member A/Rho-associated coiled-coil containing protein kinase (RhoA/ROCK) signaling-related proteins in NCM460 cells treated with DEX and/or U46619 (RhoA/ROCK agonist) and/or DSS were detected by the respective enzyme-linked immunosorbent assay (ELISA) kits and Western blot analysis. The permeability of NCM460 monolayers was examined with transepithelial electrical resistance (TEER) assay.
Results: DEX had no effect on NCM460 cell viability. However, DEX improved the viability and barrier damage and suppressed the apoptosis and inflammation of DSS-induced NCM460 cells. Correspondingly, the expression of inflammation-related proteins was reduced and the expression of tight junction proteins was increased in DSS-induced NCM460 cells after treatment with DEX. In addition, RhoA/ROCK signaling was activated in NCM460 cells induced by DSS, which was suppressed by DEX. The protective effects of DEX on DSS-indued NCM460 cells were reversed by U46619.
Conclusion: DEX improved viability and barrier damage while suppressed apoptosis and inflammation in DSS-indued NCM460 cells by inhibiting RhoA/ROCK signaling pathway.
Tricin attenuates the progression of LPS-induced severe pneumonia in bronchial epithelial cells by regulating AKT and MAPK signaling pathways
Authors: Yang Fan , Liu Wenming
Published: May 2022

Background: Pneumonia is a continuous and widespread disease with higher incidence, the effects of it on human life can be fearful. Tricin has been demonstrated to take part in the progression and development of diseases. However, the function of Tricin and its related regulatory pathways remain unclear. This study was planned to investigate the effects of Tricin on severe pneumonia.
Methods: The cell viability was detected through CCK-8 assay. The TNF-α, IL-1β and IL-6 levels were assessed through ELISA and RT-qPCR. The levels of MDA, SOD and GSH were tested through corresponding commercial kits. The protein expressions were examined through western blot.
Results: In our study, the lipopolysaccharide (LPS) was firstly used to stimulate cell model for severe pneumonia. We discovered that Tricin had no toxic effects on BEAS-2B cells and the decreased cell viability induced by LPS was relieved by a dose-dependent Tricin treatment. Additionally, through ELISA and RT-qPCR, it was uncovered that Tricin reduced the LPS-induced inflammation through regulating TNF-α, IL-1β and IL-6. Furthermore, Tricin relieved LPS-induced oxidative stress through reducing MDA level and enhancing SOD and GSH levels. Finally, it was demonstrated that Tricin retarded LPS-activated AKT and MAPK pathways.
Conclusion: Our findings revealed that Tricin attenuated the progression of LPS induced severe pneumonia through modulating AKT and MAPK signaling pathways. This discovery might afford one novel sight for the treatment of severe pneumonia.
Assessing the potential for Zn limitation of marine primary production: proteomic characterization of the low Zn stress response in marine diatoms
Authors:   , Kellogg Marissa
Published: May 2022

Marine diatoms are abundant photoautotrophic algae that contribute significantly to photosynthetic carbon fixation and export throughout the oceans. Zinc is an important micronutrient in algal metabolism, with scarce dissolved concentrations in the upper euphotic zone reflecting high biological demand. In this thesis, I investigated the response of marine diatoms to Zn scarcity to characterize metabolic mechanisms used to combat Zn stress. I began by assaying the ability to metabolically substitute cobalt (Co) in place of Zn in four diatom species and found that enhanced abilities to use Co are likely an adaptation to high surface dCo:dZn ratios in the native environment. I next demonstrated that Zn/Co metabolic substitution in diatoms is not universal using culture studies of Chaetoceros neogracile RS19, which has an absolute Zn requirement. Using global proteomic analysis, I then identified and characterized diatom ZCRP-A and ZCRP-B, a putative Zn-chaperone and membrane-tethered Zn acquisition protein, respectively, as two proteins involved in the low-Zn response. I demonstrated that these proteins are widespread in marine phytoplankton and can be deployed as protein biomarkers of Zn stress in the field. I furthermore documented both the detection of ZCRPs in the Southern Ocean and the existence of Zn/Fe co-limitation within the natural phytoplankton population in Terra Nova Bay, demonstrating that Zn co-limitation can indeed occur in the field, even in high macronutrient waters. Lastly, I explored the relative demand of Zn and cadmium (Cd) within the Southern Ocean community using stable 67Zn and 110Cd tracers, documenting a high demand for both metals during the austral 2017-2018 summer season and investigating the cycling of these elements within this important region. Overall, this dissertation provides new information regarding Zn acquisition and homeostasis mechanisms within marine algae and demonstrates that Zn co-limitation in the field is not only possible, but detectable via protein biomarkers.
Effect of Clubbell Training on Glenohumeral Internal and External Rotation, Muscle Function, and Ball Velocity in Baseball Pitchers
Authors: Choi Won-Ho , Lee Jung-No
Published: May 2022

OBJECTIVES This study examined the effects of clubbell exercises in a pitcher training program on the range of motion (ROM), muscle function, and its subsequent influence on pitching velocity. Additionally, this study aimed to develop an optimal training program to prevent injury while improving pitcher performance.METHODS Eighteen pitchers were included and equally divided into clubbell exercise and control groups. Clubbell training was conducted through 60-minute sessions twice a week for 12 weeks. Internal/external rotational (IR/ER) ROM, IR/ER muscle strength, muscle endurance, muscle power, and pitching velocity were measured before and after the intervention.RESULTS The IR (p = 0.051) and total ROM (TROM; p = 0.05) of the throwing arm decreased in the control group but increased in the clubbell exercise group, with an observed tendency of interaction. In the non-throwing arm, the ER ROM was significantly different between the two groups (p <0 .05), also with an observed tendency of interaction (p = 0.055). IR peak torque per body weight at 60°/sec significantly increased in both groups over the exercise period (p < 0.01). Total work per body weight and average power of IR at 180°/sec increased in both groups over the exercise period (p < 0.01). The pitching velocity decreased in both groups throughout the exercise period (p < 0.05).CONCLUSIONS Although 12 weeks of clubbell training had limited effects on ROM, the findings revealed that this exercise could be effective in improving pitching performance, preventing injuries, and maintaining long-term performance. Further studies are recommended for a more detailed assessment.
Optimization of Three-dimensional Face Recognition Algorithms in Financial Identity Authentication
Authors: Luo Cong , Fan Xiangbo , Yan Ying , Jin Han , Wang Xuan
Published: May 2022

Identity authentication is one of the most basic components in the computer network world. It is the key technology of information security. It plays an important role in the protection of system and data security. Biometric recognition technology provides a reliable and convenient way for identity authentication. Compared with other biometric recognition technologies, face recognition has become a hot research topic because of its convenience, friendliness and easy acceptance. With the maturity and progress of face recognition technology, its commercial application has become more and more widespread. Internet finance, e-commerce and other asset-related areas have begun to try to use face recognition technology as a means of authentication, so people’s security needs for face recognition systems are also increasing. However, as a biometric recognition system, face recognition system still has inherent security vulnerabilities and faces security threats such as template attack and counterfeit attack. In view of this, this paper studies the application of threedimensional face recognition algorithm in the field of financial identity authentication. On the basis of feature extraction of face information using neural network algorithm, K-L transform is applied to image high-dimensional vector mapping to make face recognition clearer. Thus, the image loss can be reduced.
Integration of Fuzzy with Incremental Import Vector Machine for Intrusion Detection
Authors: Kumar Ramamoorthy Arun , Karuppasamy K.
Published: May 2022

IDM design and implementation remain a difficult undertaking and an unsolved research topic. Multi-dimensional irrelevant characteristics and duplicate information are included in the network dataset. To boost the effectiveness of IDM, a novel hybrid model is developed that combines Fuzzy Genetic Algorithms with Increment Import Vector Machines (FGA-I2VM), which works with huge amounts of both normal and aberrant network data with high detecting accuracy and low false alarm rates. The algorithms chosen for IDM in this stage are machine learning algorithms, which learn, find, and adapt patterns to changing situations over time. Pre-processing is the most essential stage in any IDM, and feature selection is utilized for pre-processing, which is the act of picking a collection or subset of relevant features for the purpose of creating a solution model. Information Gain (IG) is utilized in this FGA-I2VM model to pick features from the dataset for I2VM classification. To train the I2VM classifier, FGA uses three sets of operations to produce a new set of inhabitants with distinct patterns: cross over operation, selection, and finally mutation. The new population is then put into the Import Vector Machine, a strong classifier that has been used to solve a wide range of pattern recognition issues. FGA are quick, especially considering their capacity to discover global optima. Another advantage of FGA is their naturally parallel nature of assessing the individuals within a population. As a classifier, I2VM has self-tuning properties that allow patterns to attain global optimums. The FGA-efficacy I2VM model’s is complemented by information gain, which improves speed and detection accuracy while having a low computing cost
Creatine Kinase and Lactate Dehydrogenase Enzymes Response to Lactate Tolerance Exercise Test
Authors: Rahmanian Karamatollah , Hooshmand Farhang , Shakeri Masihollah , Rahmanian Vahid , Jahromi Fatemeh Sotoodeh , Jahromi Abdolreza Sotoodeh
Published: May 2022

PURPOSE: This study aimed to assess alterations in serum creatine kinase (CK) and lactate dehydrogenase (LDH) levels after performing a lactate tolerance exercise test (LTET) in elite male swimmers.METHODS: Fourteen male adolescent swimmers participated in this study. All subjects performed LTET (8×100-meter swimming) with a 1-minute recovery interval between eight trainings. Plasma CK and LDH (markers of muscle damage) levels were measured 30 minute before and 24 hours after the test. A paired t-test was used for statistical analysis of data.RESULTS: Plasma CK and LDH levels increased immediately after LTET as compared to the values 30 minutes prior to exercise (188.91±34.04 vs. 148.83±29.63 mg/dL, p=.029; 318.17±53.89 vs. 272.08±52.93 mg/dL, p=.010, respectively). Both CK and LDH levels displayed a decreasing trend 24 hours post-LTET; however, there was no significant difference immediately after the test.CONCLUSIONS: Plasma CK and LDH levels increased following LTET, which is representative of muscle damage.
What the radiologist should know about the role of percutaneous gastrostomy: a pictorial essay
Authors: Tibana Tiago Kojun , Verza Leonardo , Rodrigues Bernardo Caetano da Silva , Monsignore Lucas Moretti , Abud Daniel Giansante , Nunes Thiago Franchi
Published: May 2022

Abstract The image-guided gastrostomy techniques, as transoral and transabdominal, can be performed when there is a failure of the endoscopic procedure or in some specific clinical scenarios. This pictorial essay intends to show the percutaneous gastrostomy techniques, indications, technical approaches, post-procedure care, and complications.
A lógica do autismo: uma análise a partir da autobiografia de Lucy Blackman
Authors: Bialer Marina
Published: May 2022

O presente artigo parte da hipótese que os autistas têm um saber sobre o funcionamento psíquico no autismo. Para demonstrá-lo, são estudados os relatos autobiográficos de Lucy Blackman, escritora autista não falante, cuja análise nos permitiu compreender a lógica das manifestações autísticas e destacou a viabilidade de uma nova regulação libidinal no autismo.
Nosografia dos Transtornos Afetivos: um recorte histórico de Kraepelin ao DSM-5
Authors: Rigolon Paula Schettino , Serpa Junior Octavio Domont de
Published: May 2022

A nosografia dos transtornos do humor e do afeto relaciona-se diretamente com as formas de apreensão dos quadros clínicos em cada contexto histórico. É indagado se o resgate histórico contribuiria para melhor compreensão e utilização da atual nosologia dos transtornos de humor. Objetiva-se realizar uma revisão histórica e crítica acerca de suas transformações conceituais e nosológicas com enfoque ao campo médico-psiquiátrico. O recorte pretendido se inicia com a proposição da “Insanidade Maníaco-Depressiva” por Emil Kraepelin em 1899, que é confrontada com as propostas pós-kraepelianas, em especial as de Karl Leonhard. Analisa-se a influência dessas na criação dos sistemas diagnósticos operacionalizados, DSM e CID. O “Transtorno Bipolar” foi um constructo introduzido pelo DSM-III e se mantém em uso até os dias atuais. Aborda-se também propostas posteriores, como a do “Espectro Bipolar”.
Espaço e experiência em restaurantes imigrantes
Authors: Bueno Alexandre Marcelo
Published: May 2022

Resumo O presente artigo se propõe a discutir algumas relações entre espaço e sujeitos a partir das possibilidades propiciadas pelos novos restaurantes imigrantes de São Paulo. O primeiro ponto a ser desenvolvido diz respeito ao modo como o espaço do restaurante é um objeto de valor construído e não uma significação dada naturalmente, de caráter espontâneo. O segundo, como o espaço do restaurante imigrante pode comportar diferentes experiências para sujeitos distintos por meio de seus papeis temáticos básicos. Ambos os pontos são desenvolvidos a partir da semiótica greimasiana e, em especial, da sociossemiótica elaborada por Eric Landowski. Ver-se-á, assim, como a alteridade, em seu fazer criador de espaços próprios, contribui para a elaboração de novas significações e de novos sentidos revividos para imigrantes e experienciados pelos brasileiros.
Michael Snow e o cinema de processos graduais
Authors: Callou Hermano Arraes
Published: May 2022

Resumo Este artigo pretende propor uma interpretação de Wavelength (1967), de Michael Snow, que privilegie a noção de processo, em detrimento da noção de estrutura, que tem sido o conceito predominante na extensa fortuna crítica do filme. A hipótese aqui apresentada é a de que podemos compreender melhor a proposta e as implicações do filme contextualizando seus procedimentos no debate da música e da arte processual nos anos 1960, a partir de uma genealogia do conceito crítico de processo e do estudo comparativo entre cinema, arte e música.
Eu te fodo ou você me fode? Performance, pornotopia e reivindicação estética da violência em Baise-moi, de Virginie Despentes e Coralie Trinh Thi
Authors: Almeida Gabriela Machado Ramos de , Zacariotti Daniel
Published: May 2022

Resumo Esse artigo apresenta uma análise do filme Baise-moi (2000), de Virginie Despentes e Coralie Trinh Thi, tomando-o como obra capaz de produzir rasuras nos modos de enunciar o desejo e certos afetos comumente ligados às convenções do “feminino”. Propomos uma articulação teórica entre as formulações de Diana Taylor sobre performance, de Paul B. Preciado sobre pornotopia e de Sam Bourcier sobre ação dissensual queer para proceder à análise de três sequências do longa-metragem. Acionamos também a obra teórica e literária da própria Despentes, que opera de forma complementar ao filme e nos ajuda a perceber como Baise-moi produz uma reivindicação estética da violência marcada por traços como o exagero, a hiperviolência gráfica e o sexo explícito.
Urgência de não ficção: enfrentamentos políticos e literários do jornalismo narrativo
Authors: Serelle Marcio
Published: May 2022

Resumo Neste artigo, investigo a declaração recorrente de escritores e jornalistas latino-americanos sobre a impossibilidade de se fazer ficção em face de contextos opressores. Analiso duas narrativas, Garotas mortas, de Selva Almada, e Os vampiros da realidade só matam pobres, de Eliane Brum, que se apresentam como relatos factuais de intervenção. Minha hipótese é de que se a ficção se apresenta como impossível, ela é também imprescindível, uma vez que esse tipo de narrativa a exige. Para isso, discuto a emergência e as ambiguidades da noção de não ficção e proponho, a partir de Jacques Rancière, o entendimento de ficção como estrutura inteligível erguida por meio do encadeamento de situações e de atores determinados, o que obscurece limites entre literatura e jornalismo. Na análise das obras, evidencio os atravessamentos do documental para o literário e o modo como certa consciência ficcional se manifesta textualmente, mesmo diante da urgência afirmada de não ficção.
“Pare de sofrer!”: os discursos da Igreja Universal sobre depressão na pandemia da covid-19
Authors: Rodrigues Emanuelle Gonçalves Brandão , Silva Amanda Borba da , Freitas Flávia Carvalho de
Published: May 2022

Resumo A proposta deste trabalho é compreender como a depressão durante a pandemia da covid-19 tem sido discursivizada pela Igreja Universal do Reino de Deus (IURD). Busca-se identificar quais compreensões sobre a depressão e a cura emergem nesses discursos. Para tanto, tomamos como objeto de análise os textos da seção Notícias do portal da IURD. O levantamento e o tratamento do corpus valeram-se de procedimentos da análise de conteúdo proposta por Bardin, para que, em seguida, a amostra fosse analisada discursivamente. A análise se apoiou em estudos de Dunker acerca do sofrimento psíquico na atualidade e de sua relação com o neoliberalismo (DARDOT, LAVAL, 2021). Os resultados apontam que a depressão é discursivizada pela IURD como um problema espiritual, que tem sua cura através da fé, endossando a responsabilidade de si como imperativo da obtenção de resultados, alinhando-se a certos discursos neoliberais.
Huillet-Straub no cinema moderno: autoria como primado do material e espectatorialidade libertária
Authors: Martins Dalila Camargo
Published: May 2022

Resumo Embora sua trajetória nomádica (decorrente do exílio) por vezes impeça uma vinculação nominal de Danièle Huillet e Jean-Marie Straub aos novos movimentos cinematográficos europeus do pós-guerra, o casal de cineastas se formou no bojo cinéfilo e crítico que daria origem à Nouvelle Vague e fomentou o surgimento do Junger Deutscher Film — além de sua obra atualizar o problema do realismo. Mediante à contextualização do início da carreira de Huillet-Straub e análises fílmicas sumárias, o objetivo deste artigo é introduzir a concepção huillet-straubiana de autoria, a qual se aproxima, mas difere, tanto da Politique des auteurs quanto do Autorenfilm, sendo caracterizada pelo primado do material e por uma espectatorialidade libertária.
O cinema contra-colonial Huni Kuin: a face velada do colonialismo
Authors: Felipe Marcos Aurélio
Published: May 2022

Resumo Este artigo aborda as perspectivas pós-coloniais do cinema sobre os povos originários, com foco na pragmática fílmica de reversão histórica Huni Kuin do realizador Zezinho Yube. O objetivo é analisar os regimes imagéticos pós-coloniais, do filme etnográfico e do cinema indígena, como contranarrativas à história oficial e as redes de silenciamento da agência nativa. O estudo é fundamentado na crítica pós-colonial, nos estudos de cinema e na perspectiva antropológica. A desconstrução dos regimes imagéticos oficiais de silenciamento da presença indígena na história resulta de uma pragmática fílmica de reversão de categoriais coloniais, que, sob a autogestão dos processos e produtos fílmicos, converge para o mesmo campo: quem filma, quem é filmado e o que é filmado, desenvolvendo, assim, um cinema originário contra-colonial.
Identifying cotton genotypes resistant to Meloidogyne incognita race 3 using Blup
Authors: Silva Edgard Henrique Costa , Candido Willame dos Santos , Santos Lucas da Silva
Published: May 2022

ABSTRACT Few genetic resistance sources to root-knot nematodes are known for cotton, and there are no cultivars combining both resistance and good agronomic attributes in Brazil. Techniques that allow an efficient selection of promising sources of genetic resistance are needed. This study aimed to identify cotton genotypes resistant to Meloidogyne incognita race 3 via REML/Blup. The experiment was completely randomized, with 32 genotypes and seven replicates, in a protected environment. The statistical model 83 was used. The root weight, final population, reproduction factor and reproduction index were estimated. The final population and reproduction index presented the highest values for genotypic variance and genetic variation coefficients, indicating a favorable situation for the selection of cotton genotypes resistant to the nematode. The mean heritability (h2mg) of the genotype was higher than 85 % for the final population, reproduction factor and reproduction index, opening a possibility of selection success based on genotype means. The genotypes CS8601, SA2572, Coodetec 404 and BJ 3128 are promising for crosses aiming the selection of genotypes resistant to the nematode.
Effect of climate smart agricultural practices on food security among farming households in Kwara State, North-Central Nigeria
Authors: Adebisi Luke Oloruntoba , Adebisi Oluwaremilekun Ayobami , Jonathan Asuquo , Oludare Olufunke Tomisona , ODUM Emmanuel Egbodo-Boheje
Published: May 2022

ABSTRACT Climate change brings a huge threat to the sustainability of food production and other livelihood activities in vulnerable areas like Nigeria, because it relies majorly on rain-fed agriculture. This study aimed to evaluate the effect of climate smart agricultural practices (CSAP) on food security of farming households in the Kwara State, North-Central Nigeria. A three-stage sampling technique was used to select ninety farming households, and a structured questionnaire to obtain information for the study. The collected data were analyzed using the food security index, adaptation strategy use index and logistic regression model. The result revealed that crop rotation is the most used CSAP in the study area, and that 16.67 % of the respondents are low users, 53.33 % medium users and 30 % high users of CSAP. It was also observed that 58.9 % of the farming households are food secured, while 41.1 % are food insecure. The logistic regression showed that the food security of the farming households is significantly affected by education, access to extension visits, farm size, off-farm income and CSAP.
Genetic divergence and physiological quality of dwarf castor bean lines seeds
Authors: Oliveira Neto Sebastião Soares de , Ramos Andréia Rodrigues , Oliveira Carlos Eduardo da Silva , Silva Edvaldo Aparecido Amaral da , Zanotto Maurício Dutra
Published: May 2022

ABSTRACT The development of new castor bean genotypes with high grain yield and physiological seed quality is fundamental to the economic success of the crop. This study aimed to evaluate the agronomic performance and physiological quality of seeds of twelve castor bean lines, in order to determine superior genotypes. Plant height, insertion height and number of racemes, grain yield, oil content, germination percentage and vigor were evaluated. The dwarf castor bean lines H4, H5 and H11 were responsible for a grain yield above 1,400 kg ha-1 and are, therefore, promising for getting new hybrids. The indirect selection of dwarf castor bean lines can be performed through morphologic traits. The lines H4, H6, H9, H11 and H12 produced seeds with superior vigor and germinative quality.
Genetic diversity of coconut germplasm native to South Kalimantan, Indonesia: a molecular study
Authors: Mursyidin Dindin Hidayatul , Ahyar Gusti Muhammad Zainal
Published: May 2022

ABSTRACT Coconut (Cocos nucifera L.) is one of the most important tree crops in the world, especially in the tropics. This study aimed to determine the genetic diversity of coconut germplasm native to South Kalimantan, Indonesia, using the rbcL marker. Nine coconut samples, being eight natively collected from this region and one as an outgroup (obtained from the GenBank database), were used in the study. According to the rbcL marker, the coconut germplasm native to South Kalimantan has a relatively high diversity, with a nucleotide diversity (π) of 0.51. The level of diversity is strongly correlated with the mutation present in the observed region, rbcL. The phylogenetic analysis showed that the coconut germplasm has a unique relationship, where the ‘Dalam’ cultivar is the closest to three other dwarf coconuts, i.e., ‘Genjah Kuning 1’, ‘Genjah Kuning 3’ and ‘Wulung’.
Musing over the role of emotions to promote the exodus from the Comfort Zone in English language teaching and learning at a state university in Brazil
Authors: Gomes Gysele da S. Colombo
Published: May 2022

ABSTRACT This paper focuses on the development of an empirical study over the Comfort Zone ( 2004; 2006; 2018), shedding light on the role played by emotions in empowering learners to confidently interact in English classes at a public university in Rio de Janeiro, in Brazil. The theoretical framework was based on the concept of the Comfort Zone (2004; 2006; 2018); and that of emotions (ARAGÃO, 2004; 2011; BARCELOS, 2018; MATURANA, 2002; ZEMBYLAS 2004; 2018). This qualitative and interpretive study (DENZIN; LINCOLN, 2006; LINCOLN; GUBA, 2006) used an individual questionnaire, field observations, individual participants’ reports of observation of classes and peers’ performance, individual exploratory conversations and an ethnographic group interview (ERICKSON, 1986) for debriefing, and a final individual diary of participants as tools for data collection. Results display a range of emotions impacting learners’ performance in interaction.1
Nuevos registros del género Geastrum (Agaricomycetes, Basidiomycota) para Colombia
Authors: Pinzón-Osorio César Augustio , Backes-Schuster Martina Heloisa , Carrero-Torres Claudia Carina
Published: May 2022

Se registra por primera vez a Geastrum minimum y G. pectinatum para Colombia. Los basidiomas fueron colectados en un bosque húmedo montano bajo (bh-MB) de los Cerros Orientales de Bogotá, Departamento de Cundinamarca. Las especies son descritas e ilustradas y se aporta información sobre su distribución, ecología y hábitat.
Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes v1
Authors: Gogoi Prerana , Shiozaki Momoko , Gouaux Eric
Published: May 2022

Ionotropic glutamate receptors (iGluRs) at postsynaptic terminals mediate the majority of fast excitatory neurotransmission in response to release of glutamate from the presynaptic terminal. Obtaining structural information on the molecular organization of iGluRs in their native environment, along with other signaling and scaffolding proteins in the postsynaptic density (PSD), and associated proteins on the presynaptic terminal, would enhance understanding of the molecular basis for excitatory synaptic transmission in normal and in disease states. Cryo-electron tomography (ET) studies of synaptosomes is one attractive vehicle by which to study iGluR-containing excitatory synapses. Here we describe a workflow for the preparation of glutamatergic synaptosomes for cryo-ET studies. We describe the utilization of fluorescent markers for the facile detection of the pre and postsynaptic terminals of glutamatergic synaptosomes using cryo-laser scanning confocal microscope (cryo-LSM). We further provide the details for preparation of lamellae, between ~100 to 200 nm thick, of glutamatergic synaptosomes using cryo-focused ion-beam (FIB) milling. We monitor the lamella preparation using a scanning electron microscope (SEM) and following lamella production, we identify regions for subsequent cryo-ET studies by confocal fluorescent imaging, exploiting the pre and postsynaptic fluorophores.
Immunofluorescent staining of pancreatic sections v1
Authors: Zarrouki Bader , Moullé Valentine , Poitout Vincent , CT Tremblay Caroline , Ghislain Julien
Published: May 2022

This protocol describes the steps for performing fluorescence immunohistochemistry on fixed-frozen pancreatic tissue sections. It is suitable for pancreatic tissue isolated from rats and mice at postnatal to adult stages. We routinely apply this protocol to quantify total and proliferating or apoptotic beta-, alpha- and delta-cells. Briefly, pancreata are fixed in 4% paraformaldehyde solution and cryoprotected overnight in 30% sucrose. Tissue are then embedded, frozen, sectioned and mounted on slides. Antigen retrieval is performed using sodium citrate buffer prior to immunostaining.
English as a Foreign Language in Saudi Arabia: Learners’ Evaluation of Their Language Experience
Authors: Almesaar Oun
Published: May 2022

This study focused on the understudied emotional side of learning. Specifically, it aimed at understanding the quality of the English learning journey of seven Saudi Arabian college students. For this purpose, a survey was conducted first to collect personal data, sociolinguistic data, curricular and other experiences in the path of learning English. Then, this was followed by three qualitative instruments to collect data presented in narratives, journals, and interviews. Qualitative data were analyzed inductively and deductively by thematic analysis. It was found that participants evaluated their English language learning journey in college using more positive emotional states than negative ones. These states, both positive and negative, affected participants’ reactions towards their English experience, with pleasant encounters boosting their motivation and unpleasant encounters decreasing their motivation. Moreover, results indicated that participants were dissatisfied with their English language skills, even though they seemed to focus more on their successes than on their failures. The resulting insights can assist teachers in understanding the importance of a positive and supporting English language learning environment and its impact on increasing learners’ performance and motivation.
Syntactic, Semantic and Discourse Effects on the Processing of Scrambled Japanese Sentences
Authors: Shoji Shinichi
Published: May 2022

In Japanese sentences, the default word order is ‘subject-object-verb’ (SOV). However, Japanese allows scrambling of noun phrases (NPs), for example ‘object-subject-verb’ (OSV) as opposed to the unscrambled default order. Two self-paced reading experiments in the moving window paradigm were conducted to test the effects of syntax, semantics and discourse in native Japanese and native English speakers’ processing of scrambled Japanese sentences. The first experiment examined how the syntactic factor (NP order) and semantic factor (NP animacy) affect processing of Japanese sentences. Results revealed that animacy difference between the subject-NP and object-NP contributes more to native English speakers’ processing than default SOV word order, whereas no such difference was found for native Japanese speakers. The second experiment examined the discourse effect in processing of scrambled OSV sentences. The experiment revealed that the processing of Japanese scrambled sentences is facilitated by the preceding context for both native Japanese and English speakers.
A Classroom-Centered Study of Third Tone in Mandarin Chinese
Authors: Li Bianye
Published: May 2022

Phonological third tone sandhi studies in Mandarin Chinese will lead more often to a lab-centered research, whereas the incorporation of phonetic tone sandhi studies into phonological analyses will shed a light on a classroom-centered study. This incorporation suggests a revised approach to the third tone sandhi from an articulatory perspective. As a result, the study of pitch values and pitch contours of a third tone are taken over by the study of sound positions and jaw/chin movements. The well-known five-level tonal diagram is challenged and replaced by a seven-level tonal diagram with an application of only two forms of pronouncing a third tone ---pseudo third tone and pseudo second tone. All these aim to investigate a classroom-centered study of a third tone as an understudied area to provide practical guidance for Mandarin instructors and learners of Mandarin as second language.
Integration of Perceptual Similarity With Faithful Mapping of Phonological Contrast in Loanword Adaptation: Mandarin Chinese Adaptation of English Stops
Authors: He Mosi , He Jianing
Published: May 2022

In loanword phonology, perceptual similarity and faithful mapping of phonological contrast are two main factors which influence loanword adaptation. Previous studies observe that English phonological voicing contrast is mapped to Mandarin Chinese (hereafter, Mandarin) phonological aspiration contrast, indicating faithful mapping of phonological contrast. Nevertheless, the role of perceptual similarity in adaptation of English stops to Mandarin has not been fully explored. The current study investigates the influence of perceptual similarity on loanword adaptation by examining how English voiced and voiceless stops are adapted in Mandarin Chinese using a data set of 1427 novel Mandarin loanwords from English. The results show consistent adaptation of English voiced stops as Mandarin unaspirated stops and English aspirated voiceless stops as Mandarin aspirated ones, while inconsistent adaptation patterns are found for the English unaspirated voiceless stops. In particular, English post-/s/ unaspirated voiceless stops which occupy a similar voice onset time (VOT) region to Mandarin unaspirated stops are adapted as Mandarin unaspirated ones, whereas the rest are mapped to aspirated stops in Mandarin. The overall adaptation patterns provide partial support to faithful mapping of phonological contrast and provide robust evidence for an integration of perceptual similarity with faithful mapping of phonological contrast in loanword adaptation.
Proposed 21st Century Learning Themes in English Classrooms
Authors: Alawiah Tuti , Yassi Abdul Hakim , Nasmilah  , Pammu Abidin
Published: May 2022

In many universities in Indonesia, English is still a mandatory subject. The universities provide the courses under the names Bahasa Inggris (English), English for Academic purposes, English I to II, English for Proficiency, English for Health Science, etc. Different universities give 2 credits, 3 credits, or more than 4 credits. The teaching materials used are from various sources, such as from the internet, English books published by famous publishers, or modules written by the lecturers. However, the materials provided are often not suitable for the current situation. Therefore, this research was conducted to investigate and seek better alternatives for English learning. What are the students’ target needs with respect to English class materials development? What are the students’ learning needs with respect to the development of English class material for Social and Political Science’s Students? And what suitable materials are available that are in line with 21st-century learning? This study surveyed 437 new students in the first semester from the Social and Political Science Faculty and eight English lecturers National University of Indonesia. Google form questionnaires were used to collect and analyze data. The three main indicators to be analyzed were: students’ present situation, students’ target situation, and students’ learning situation. The results show that 60.9% agree that the topics discussed relate to 21st-century learning, 80.3% of all necessary English skills are learned by the students, and they prefer learning from YouTube/social media and academic journals. Nearly half (46.9 %) were comfortable with learning through blended learning, and 95.6% agreed with the use of Google meet, Google classroom, and Zoom meetings. Furthermore, the materials considered appropriate to 21st-century learning include global awareness, finance, economics, business and entrepreneurial literacy, civic literacy, health literacy, and environmental literacy.
Teachers’ Perspectives on Foreign Language Acquisition and Mobile or Computer Assisted Language Learning: A Qualitative Study
Authors: Holbah Wael A.
Published: May 2022

The study underscores issues of teachers' perspectives, their understanding, awareness, utilization, and effectiveness of technological mediated mobile capabilities in foreign language acquisition (FLA). Seven teachers (FLA) from five nationalities were interviewed online, and the data collected and compiled along with notes were descriptively analyzed. The findings illustrate that although FLA teachers are ready to embrace changes and accept the usage of technology-mediated computer and mobile-assisted language learning (C/MALL) in online classes; however, this adaptability posits some challenges, which need technical support, training, interactions on its usage for language acquisition. The argument emphasizes the discourses between FLA teachers, parents, and institutions for creating a conducive environment and technological affordances for executing computer and mobile-assisted language learning in significant ways. Besides, it highlights the contradiction between the usage of Smart Mobile Devices (SMDs) for language acquisition and lack of efficiency and effective use of such devices by teachers, and the formalities of mobile use for teachers to include student-centered activities and students’ immersion. Finally, the study presents implications and prospects for future studies based on the perception of FLA teachers, and parents towards the use of the computer or SMDS viewed as CALL or MALL.
Conception of the adaptive functional dominant of central nervous system in the prediction of neurorehabilitation results
Authors: Starchenko A. A. , Khlunovsky A. N.
Published: May 2022

The conception of the adaptive functional dominant of central nervous system for the description of general regularities of the patho- and sanogenesis course in neurosurgicai patients is proposed. It is shown that the disease result and primary trend of rehabilitation depend on the developing adaptive dominant.
State of the control mechanisms of circular provision of the brain in patients with cerebral tumors and pharmacologic rehabilitation of their disorders
Authors: Danilov V. I. , Shulman K. M. , Studentsova I. A. , Ismagilov M. F.
Published: May 2022

Dimerhosphone (15% solution in a dose of one table spoon 3 times per day) normalizes the control mechanisms of circular provision of the brain in patients with cerebral tumors.
Medico-psychologic service and socio- medical rehabilitation of convicted persons under age with psychic pathology
Authors: Vostroknutov N. V. , Guryeva V. A. , Posokhova V. I.
Published: May 2022

It is established that the index of occu- rance of psychic disfunctions among convicted persons under age comprises 53,7%, under these conditions the stable structure of the following psychic disfunctions is stated: residual phenomena of the organic disorder of the brain of various genesis (29,6%), psychopathies (13,7%), oligophrenia (8,8%), schixophrenia (0,9%). The program of socio-mdical rehabilitation of convicted persons under age with psychic disfunctions is presented.
Rehabilitation of patients with traumatic and compressive neuropathies
Authors: Bobzin V. S. , Saikova L. A. , Kosachev V. D. , Pustozerov V. G. , Vasilyeva T. N.
Published: May 2022

The experience of recovery and rehabilitation of patients with hereditary neuromuscular diseases (more than 1000 observations) is generalized. Their own methods of pathogenetic and correcting therapy as well as patented methods are described. Their therapeutic efficacy is estimated on evidence of clinical observations, biochemical and electrophysiologic examinations. The possibility to obtain the stable and relatively prolonged remissions and to bring the disease under control is shown.
Paroxysmal course of Nishimoto-Takeuchi-Kudo disease (my-my disease)
Authors: Danilov V. I. , Zakirova N. G. , Tavluy K. P.
Published: May 2022

My-my disease was described in 1966 as a clinical and radiological syndrome by Japanese authors. Life-time diagnosis of this disease is possible only with the help of angiographic examination, which reveals its main symptom - stenosis of the supra-sphenoid section of the internal carotid artery, often on both sides. As a rule, stenotic changes also extend to the first segments of the anterior and middle cerebral arteries. The distal branches of these arteries are not affected and allow collateral blood flow.
Pain syndromes and paresthesias of the oral cavity
Authors: Grechko V. E.
Published: May 2022

Among those who seek help from dentists, a special group is made up of patients who complain of pain and discomfort in the tongue, less often in the area of the palate, the inner surfaces of the cheeks, or in other parts of the oral cavity. This disease was first designated as glossodynia, and then as glossalgia [2]. Later, in clinical practice, they begin to use the term "stomalgia", since it turned out that pain and paresthesia are localized not only in the tongue, but also in other parts of the oral cavity.
Key problems of modern forensic psychiatry
Authors: Shostakovich B. V.
Published: May 2022

Forensic psychiatry can be defined as a branch of medical science - psychiatry, whose task is to establish the mental state of a person in relation to certain legal norms and situations. Such a definition implies several main areas of activity of forensic psychiatrists and scientific research in this area. The inseparable connection between forensic psychiatry and general psychiatry, the impossibility of fruitful work of expert psychiatrists in isolation from the psychiatric clinic, without the use of an appropriate conceptual apparatus, without taking into account the development trends of diagnostics, therapy and organization, remains fundamental. Obviously, the specific tasks of forensic psychiatry at the present stage are directly related to the tasks of general psychiatry and a number of changing legal provisions, and complex integrative relations between psychiatry and law are necessary here.
Message from the editor-in-chief
Authors: Shaposhnikov Yu. G.
Published: May 2022

On behalf of the editorial board and editorial board, I would like to congratulate my colleagues on the publication of the new journal Bulletin of Traumatology and Orthopedics named after V.I. N. N. Priorov "
Quantitative Analysis of the Interstructural Space of the Compact Substance of Human Bone
Authors: Omelyanenko N. P. , Butyrin G. M.
Published: May 2022

Central Institute of Traumatology and Orthopedics. N.N. Priorova, NIIGrafit, Moscow With the help of a methodological complex - electron microscopy, mercury porosimetry, gas adsorption - and subsequent analytical data processing, new quantitative parameters of the interstructural space of the compact substance of human bone were obtained
Operation for harm to well - ikonomov as one of the methods of organ-preserving surgery for bone tumors
Authors: Makhson A. N. , Burdygin V. N.
Published: May 2022

Among the various methods of treating patients with tumors of the skin, a special place is occupied by the Vreden-Ikonomov method. It consists in resection of the affected part of the bone, its digestion (boiling) and replantation
Information
Authors: Board Editorial
Published: May 2022

SI KOT (SICOT - Societe Internationale de Chirurgie Orthopedique et de Traumatologic) The largest international society of orthopedics and traumatology, founded in October 1929 in Paris, currently unites more than 2500 specialists from 81 countries. As stated in the Charter of the society, SICOT sees its goal in its work in orthopedics and traumatology, in support of young orthopedists and traumatologists, in favor of the exchange of experience and establishment of relationships between orthopedists and traumatologists from different countries