bluuebunny/crossref_metadata_2025 huggingface dataset:
Adjunct n-3 Long-Chain Polyunsaturated Fatty Acid Treatment in Tuberculosis Reduces Inflammation and Improves Anemia of Infection More in C3HeB/FeJ Mice With Low n-3 Fatty Acid Status Than Sufficient n-3 Fatty Acid Status
Authors: Hayford Frank E. A. , Dolman Robin C. , Ozturk Mumin , Nienaber Arista , Ricci Cristian , Loots Du Toit , Brombacher Frank , Blaauw Renée , Smuts Cornelius M. , Parihar Suraj P. , Malan Linda
Published: August 2021

Populations at risk for tuberculosis (TB) may have a low n-3 polyunsaturated fatty acid (PUFA) status. Our research previously showed that post-infection supplementation of n-3 long-chain PUFA (LCPUFA) in TB without TB medication was beneficial in n-3 PUFA sufficient but not in low-status C3HeB/FeJ mice. In this study, we investigated the effect of n-3 LCPUFA adjunct to TB medication in TB mice with a low compared to a sufficient n-3 PUFA status. Mice were conditioned on an n-3 PUFA-deficient (n-3FAD) or n-3 PUFA-sufficient (n-3FAS) diet for 6 weeks before TB infection. Post-infection at 2 weeks, both groups were switched to an n-3 LCPUFA [eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA)] supplemented diet and euthanized at 4- and 14- days post-treatment. Iron and anemia status, bacterial loads, lung pathology, lung cytokines/chemokines, and lung lipid mediators were measured. Following 14 days of treatment, hemoglobin (Hb) was higher in the n-3FAD than the untreated n-3FAS group (p = 0.022), whereas the n-3FAS (drug) treated control and n-3FAS groups were not. Pro-inflammatory lung cytokines; interleukin-6 (IL-6) (p = 0.011), IL-1α (p = 0.039), MCP1 (p = 0.003), MIP1- α (p = 0.043), and RANTES (p = 0.034); were lower, and the anti-inflammatory cytokine IL-4 (p = 0.002) and growth factor GMCSF (p = 0.007) were higher in the n-3FAD compared with the n-3FAS mice after 14 days. These results suggest that n-3 LCPUFA therapy in TB-infected mice, in combination with TB medication, may improve anemia of infection more in low n-3 fatty acid status than sufficient status mice. Furthermore, the low n-3 fatty acid status TB mice supplemented with n-3 LCPUFA showed comparatively lower cytokine-mediated inflammation despite presenting with lower pro-resolving lipid mediators.
RanBP3 Regulates Proliferation, Apoptosis and Chemosensitivity of Chronic Myeloid Leukemia Cells via Mediating SMAD2/3 and ERK1/2 Nuclear Transport
Authors: Li Qian , Huang Zhenglan , Peng Yuhang , Wang Xin , Jiang Guoyun , Wang Teng , Mou Ke , Feng Wenli
Published: August 2021

Abnormal subcellular localization of proteins is an important cause of tumorigenesis and drug resistance. Chromosome region maintenance 1 (CRM1), the nuclear export regulator of most proteins, has been confirmed to be over-expressed in various malignancies and is regarded as an efficient target. But the potential role of the CRM1 cofactor RanBP3 (Ran Binding Protein 3) is left unrevealed in chronic myeloid leukemia (CML). Here, we first detected the level of RanBP3 in CML and found an elevated RanBP3 expression in CML compared with control. Then we used shRNA lentivirus to down-regulated RanBP3 in imatinib sensitive K562 cells and resistant K562/G01 cells and found RanBP3 silencing inhibited cell proliferation by up-regulating p21, induced caspase3-related cell apoptosis, and enhanced the drug sensitivity of IM in vitro. Notably, we observed that RanBP3 silencing restored imatinib sensitivity of K562 cells in NOD/SCID mice. Mechanistically, the nuclear aggregation of SMAD2/3 revealed that tumor suppressor axis (TGF-β)-SMAD2/3-p21 was the anti-proliferation program related to RanBP3 knockdown, and the decrease of cytoplasmic ERK1/2 caused by RanBP3 interference leaded to the down-regulation of anti-apoptosis protein p(Ser112)-BAD, which was the mechanism of increased cell apoptosis and enhanced chemosensitivity to imatinib in CML. In summary, this study revealed the expression and potential role of RanBP3 in CML, suggesting that targeting RanBP3 alone or combined with TKIs could improve the clinical response of CML.
Circulating Cell-Free DNA-Based Detection of Tumor Suppressor Gene Copy Number Loss and Its Clinical Implication in Metastatic Prostate Cancer
Authors: Dong Xiaoxi , Zheng Tiantian , Zhang Minhua , Dai Chao , Wang Lili , Wang Lei , Zhang Ruipeng , Long Yuan , Wen Danyi , Xie Feng , Zhang Yue , Huang Yong , Dong Jianguo , Liu Huan , Du Pan , King Bonnie L. , Tan Winston , Jia Shidong , Lu Chris X. , Kohli Manish , Wang Haitao , Yu Jianjun
Published: August 2021

Current liquid biopsy assays lack sufficient sensitivity to detect copy number loss, which limits the interrogation of critical tumor suppressor gene deletions during cancer progression and treatment. Here we describe a liquid biopsy assay with improved sensitivity for detection of copy number loss in blood samples with low levels of circulating tumor DNA, and demonstrate its utility by profiling PTEN, RB1, and TP53 genetic loss in metastatic prostate cancer patients.
Lower Expression of miR-26a in PBMCs Indicates the Occurrence of Early-Onset Neonatal Sepsis and Is Partly Mediated by the Upregulation of PTEN
Authors: Zhao Ziyan , Zhang Jiajie
Published: August 2021

Aim: It is difficult to identify neonatal sepsis early due to the lack of specific markers. The aim of the present study was to explore whether miR-26a expression in peripheral blood mononuclear cells (PBMCs) could be used as a diagnostic marker of the disease and whether phosphatase and tensin homolog (PTEN) was involved in suppressing miR-26a expression.

Methods: A total of 51 early-onset septic newborns and 102 healthy newborns were included. Blood specimens were collected from septic newborns at the time of clinical diagnosis (baseline) and again between 72 and 96 h after birth. Blood specimens were collected from healthy newborns on admission. The expressions of miR-26a and PTEN in PBMCs were measured using real-time quantitative PCR (RT-qPCR). Other data, including hemoculture, were collected from medical records.

Results: In septic newborns with and without a positive hemoculture, a lower baseline level of miR-26a in PBMCs was associated with a higher risk of disease. Additionally, at baseline, there was a certain linear relationship between the levels of miR-26a and two serological inflammatory markers (i.e., white blood cell count and C-reactive protein level) in septic newborns. In addition, the baseline expressions of miR-26a and PTEN showed a reverse linear relationship. Compared with those at baseline, the expression of miR-26a was higher and the expression of PTEN was lower in septic newborns starting at 72 h after birth.

Conclusion: A lower baseline miR-26a expression in PBMCs indicated the occurrence of early-onset neonatal sepsis, and a reduced miR-26a expression might be partly related to the inflammatory process and PTEN upregulation.
Interleukin-6: A Novel Target for Cardio-Cerebrovascular Diseases
Authors: Su Jian-Hui , Luo Meng-Yi , Liang Na- , Gong Shao-Xin , Chen Wei , Huang Wen-Qian , Tian Ying , Wang Ai-Ping
Published: August 2021

Cardio-Cerebrovascular Disease is a collective term for cardiovascular disease and cerebrovascular disease, being a serious threat to human health. A growing number of studies have proved that the content of inflammatory factors or mediators determines the stability of vascular plaque and the incidence of cardio-cerebrovascular event, and involves in the process of Cardio-Cerebrovascular Diseases. Interleukin-6 is a widely used cytokine that causes inflammation and oxidative stress, which would further result in cardiac and cerebral injury. The increased expression of interleukin-6 is closely related to atherosclerosis, myocardial infarction, heart failure and ischemic stroke. It is a key risk factor for these diseases by triggering inflammatory reaction and inducing other molecules release. Therefore, interleukin-6 may become a potential target for Cardio-Cerebrovascular Diseases in the future. This paper is aimed to discuss the expression changes and pathological mechanisms of interleukin-6 in Cardio-Cerebrovascular Diseases, and to provide a novel strategy for the prevention and treatment of Cardio-Cerebrovascular Diseases.
Mechanical Stimulation-Induced Calcium Signaling by Piezo1 Channel Activation in Human Odontoblast Reduces Dentin Mineralization
Authors: Matsunaga Mayumi , Kimura Maki , Ouchi Takehito , Nakamura Takashi , Ohyama Sadao , Ando Masayuki , Nomura Sachie , Azuma Toshifumi , Ichinohe Tatsuya , Shibukawa Yoshiyuki
Published: August 2021

Odontoblasts play critical roles in dentin formation and sensory transduction following stimuli on the dentin surface. Exogenous stimuli to the dentin surface elicit dentinal sensitivity through the movement of fluids in dentinal tubules, resulting in cellular deformation. Recently, Piezo1 channels have been implicated in mechanosensitive processes, as well as Ca2+ signals in odontoblasts. However, in human odontoblasts, the cellular responses induced by mechanical stimulation, Piezo1 channel expression, and its pharmacological properties remain unclear. In the present study, we examined functional expression of the Piezo1 channel by recording direct mechanical stimulation-induced Ca2+ signaling in dentin matrix protein 1 (DMP-1)-, nestin-, and dentin sialophosphoprotein (DSPP)-immunopositive human odontoblasts. Mechanical stimulation of human odontoblasts transiently increased intracellular free calcium concentration ([Ca2+]i). Application of repeated mechanical stimulation to human odontoblasts resulted in repeated transient [Ca2+]i increases, but did not show any desensitizing effects on [Ca2+]i increases. We also observed a transient [Ca2+]i increase in the neighboring odontoblasts to the stimulated cells during mechanical stimulation, showing a decrease in [Ca2+]i with an increasing distance from the mechanically stimulated cells. Application of Yoda1 transiently increased [Ca2+]i. This increase was inhibited by application of Gd3+ and Dooku1, respectively. Mechanical stimulation-induced [Ca2+]i increase was also inhibited by application of Gd3+ or Dooku1. When Piezo1 channels in human odontoblasts were knocked down by gene silencing with short hairpin RNA (shRNA), mechanical stimulation-induced [Ca2+]i responses were almost completely abolished. Piezo1 channel knockdown attenuated the number of Piezo1-immunopositive cells in the immunofluorescence analysis, while no effects were observed in Piezo2-immunopositive cells. Alizarin red staining distinctly showed that pharmacological activation of Piezo1 channels by Yoda1 significantly suppressed mineralization, and shRNA-mediated knockdown of Piezo1 also significantly enhanced mineralization. These results suggest that mechanical stimulation predominantly activates intracellular Ca2+ signaling via Piezo1 channel opening, rather than Piezo2 channels, and the Ca2+ signal establishes intercellular odontoblast-odontoblast communication. In addition, Piezo1 channel activation participates in the reduction of dentinogenesis. Thus, the intracellular Ca2+ signaling pathway mediated by Piezo1 channels could contribute to cellular function in human odontoblasts in two ways: (1) generating dentinal sensitivity and (2) suppressing physiological/reactional dentinogenesis, following cellular deformation induced by hydrodynamic forces inside dentinal tubules.
GRIA2/ENPP3 Regulates the Proliferation and Migration of Vascular Smooth Muscle Cells in the Restenosis Process Post-PTA in Lower Extremity Arteries
Authors: Zhou Mi , Qi Lixing , Gu Yongquan
Published: August 2021

Restenosis is the main restriction on the long-term efficacy of percutaneous transluminal angioplasty (PTA) therapy for peripheral artery disease (PAD). Interventions to prevent restenosis are poor, and the exact mechanism is unclear. Here, we aimed to elucidate the role of GRIA2 in the restenosis process post-PTA in lower extremity arteries. We searched the differentially expressed genes (DEGs) between atherosclerotic and restenotic artery plaques in the Gene Expression Omnibus (GEO), and five DEGs were identified. Combined with Gene Ontology (GO) enrichment analysis, GRIA2 was significantly correlated with the restenosis process. Tissue samples were used to examine GRIA2 expression by immunofluorescence staining of atherosclerotic and restenotic artery plaques. The regulation of GRIA2 in vascular smooth muscle cells (VSMCs) was confirmed by lentiviral transfection. Overexpression of GRIA2 promoted the proliferation and migration of VSMCs. Using Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and protein–protein interaction (PPI) network, a strong connection between ENPP3 and GRIA2 was discovered. In vitro results showed that the high expression of GRIA2 in VSMCs enhanced the expression of ENPP3, while downregulation of GRIA2 downregulated ENPP3. GRIA2 is highly differentially expressed in restenotic arterial plaques, promoting the proliferation and migration of VSMCs through upregulation of ENPP3. These discoveries will help us to obtain a better understanding of restenosis in lower extremity arteries.
Atlantic Salmon (Salmo salar) Cage-Site Distribution, Behavior, and Physiology During a Newfoundland Heat Wave
Authors: Gamperl Anthony K. , Zrini Zoe A. , Sandrelli Rebeccah M.
Published: August 2021

Background: Climate change is leading to increased water temperatures and reduced oxygen levels at sea-cage sites, and this is a challenge that the Atlantic salmon aquaculture industry must adapt to it if it needs to grow sustainably. However, to do this, the industry must better understand how sea-cage conditions influence the physiology and behavior of the fish.

Method: We fitted ~2.5 kg Atlantic salmon on the south coast of Newfoundland with Star-Oddi milli-HRT ACT and Milli-TD data loggers (data storage tags, DSTs) in the summer of 2019 that allowed us to simultaneously record the fish's 3D acceleration (i.e., activity/behavior), electrocardiograms (and thus, heart rate and heart rate variability), depth, and temperature from early July to mid-October.

Results: Over the course of the summer/fall, surface water temperatures went from ~10–12 to 18–19.5°C, and then fell to 8°C. The data provide valuable information on how cage-site conditions affected the salmon and their determining factors. For example, although the fish typically selected a temperature of 14–18°C when available (i.e., this is their preferred temperature in culture), and thus were found deeper in the cage as surface water temperatures peaked, they continued to use the full range of depths available during the warmest part of the summer. The depth occupied by the fish and heart rate were greater during the day, but the latter effect was not temperature-related. Finally, while the fish generally swam at 0.4–1.0 body lengths per second (25–60 cm s−1), their activity and the proportion of time spent using non-steady swimming (i.e., burst-and-coast swimming) increased when feeding was stopped at high temperatures.

Conclusion: Data storage tags that record multiple parameters are an effective tool to understand how cage-site conditions and management influence salmon (fish) behavior, physiology, and welfare in culture, and can even be used to provide fine-scale mapping of environmental conditions. The data collected here, and that in recent publications, strongly suggest that pathogen (biotic) challenges in combination with high temperatures, not high temperatures + moderate hypoxia (~70% air saturation) by themselves, are the biggest climate-related challenge facing the salmon aquaculture industry outside of Tasmania.
Utilizing Plant Synthetic Biology to Improve Human Health and Wellness
Authors: Barnum Collin R. , Endelman Benjamin J. , Shih Patrick M.
Published: August 2021

Plants offer a vast source of bioactive chemicals with the potential to improve human health through the prevention and treatment of disease. However, many potential therapeutics are produced in small amounts or in species that are difficult to cultivate. The rapidly evolving field of plant synthetic biology provides tools to capitalize on the inventive chemistry of plants by transferring metabolic pathways for therapeutics into far more tenable plants, increasing our ability to produce complex pharmaceuticals in well-studied plant systems. Plant synthetic biology also provides methods to enhance the ability to fortify crops with nutrients and nutraceuticals. In this review, we discuss (1) the potential of plant synthetic biology to improve human health by generating plants that produce pharmaceuticals, nutrients, and nutraceuticals and (2) the technological challenges hindering our ability to generate plants producing health-promoting small molecules.
Teaching and Learning Modalities in Higher Education During the Pandemic: Responses to Coronavirus Disease 2019 From Spain
Authors: Verde Ana , Valero Jose Manuel
Published: August 2021

The effects of the pandemic have affected and continue to affect education methods every day. The education methods are not immune to the pandemic periods we are facing, so teachers must know how to adapt their methods in such a way that teaching, and its quality, is not negatively affected. This study provides an overview of different types of teaching methodology before, during, and after the coronavirus disease 2019 (COVID-19) pandemic. This study describes the different types of teaching (e.g., presence learning, blended learning, and distance education) used in two Spanish Universities (i.e., one private and one public) during the pandemic. A new teaching methodology is proposed. The purpose of this study report is to share what we learned about the response to COVID-19. Results provide a basis for reflection about the pros and cons of teaching and learning modalities in higher education. The current situation demands that we continue to rethink what is the best methodology for teaching so that the education of students is not affected in any way. This study is useful for learning about different teaching methods that exist and which ones may suit us best depending on the context, situation, and needs of our students.
From Identity to Enaction: Identity Behavior Theory
Authors: Simons Jack D.
Published: August 2021

The article deals with the meaning of identity in action regulation. A strengths-based action model, Identity Behavior Theory (IBT), is concerned with the role that identity plays in the prediction of behavioral enaction, and implications for education, science, and clinical practice. With this respect the article explores and discusses how enacted behavior, including intention and action, depends on level of subscription to identity as well as on resilience and attitudes that are related to such a behavior. The article also illustrates fields of application of IBT, use of IBT with underrepresented and marginalized groups, and as an instrument for assessing and testing possible effects of resilience, attitudes, and identity on the enacted behavior. IBT is now used to examine behavior in a variety of educational contexts in the United States, and more studies are needed to satisfactorily validate application of the model empirically.
An Examination of Risk Factors for Tobacco and Cannabis Smoke Exposure in Adolescents Using an Epigenetic Biomarker
Authors: Andersen Allan , Gerrard Meg , Gibbons Frederick X. , Beach Steven R. H. , Philibert Robert
Published: August 2021

Objective: Evolving patterns of nicotine and cannabis use by adolescents require new tools to understand the changing epidemiology of these substances. Here we describe the use of a novel epigenetic biomarker sensitive to both tobacco and cannabis smoke in a longitudinal sample of high-risk adolescents. We examine risk factors for positivity for this epigenetic biomarker in comparison to positivity for conventional serum biomarkers of nicotine and cannabis use.

Method: Eastern Iowa 10th graders who had a friend or family member who smoked were eligible to participate in a longitudinal study over 10–12th grades. Subjects provided self-report data on nicotine, tobacco, and cannabis use patterns as well as blood samples that were used for serum cotinine and THC assays. DNA was prepared for analysis of methylation at the CpG cg05575921, a sensitive indicator of smoke exposure. Relationships between positivity for each these biomarkers and a variety of risk factors, including demographics, family and peer relationships, psychopathology, willingness to smoke, and perceptions of typical cigarette and cannabis users, were examined at the 10th (n = 442), 11th (n = 376), and 12th (n = 366) grade timepoints.

Results: A increasing proportion of subjects were positive for cotinine (5–16%), THC (3–10%), and cg05575921 methylation (5–7%) across timepoints, with some overlap. Self-reported combusted tobacco and cannabis use was strongly correlated with all biomarkers, whereas cg05575921 methylation was not correlated with reported e-cigarette use. Dual users, defined as those positive for nicotine and THC in the 12th grade showed the greatest cumulative smoke exposure, indicated by cg05575921 methylation. Subjects reported more positive attitudes toward cannabis users than cigarette smokers, and willingness to smoke and positive perceptions of tobacco and cannabis smokers were significant risk factors for biomarker positivity across timepoints.

Conclusion: We conclude that measurement of cg05575921 methylation in adolescents is a useful tool in detecting tobacco smoking in adolescents, and may be a novel tool for the detection of cannabis smoking and cannabis and tobacco co-use, though non-combusted forms of nicotine use do not appear to be detectable by this method.
A Phase II Randomized, Double-Blind, Placebo-Controlled Study of the Efficacy, Safety, and Tolerability of Arbaclofen Administered for the Treatment of Social Function in Children and Adolescents With Autism Spectrum Disorders: Study Protocol for AIMS-2-TRIALS-CT1
Authors: Parellada Mara , San José Cáceres Antonia , Palmer Melanie , Delorme Richard , Jones Emily J. H. , Parr Jeremy R. , Anagnostou Evdokia , Murphy Declan G. M. , Loth Eva , Wang Paul P. , Charman Tony , Strydom Andre , Arango Celso
Published: August 2021

Background: Autism Spectrum Disorder (ASD or autism) is characterized by difficulties in social communication and interaction, which negatively impact on individuals and their families' quality of life. Currently no pharmacological interventions have been shown to be effective for improving social communication in autism. Previous trials have indicated the potential of arbaclofen for improving social function among autistic children and adolescents with fluent speech. The AIMS2TRIALS-Clinical Trial 1 (AIMS-CT1) will examine whether arbaclofen is superior to placebo in improving social function and other secondary outcomes over 16 weeks, along with safety and tolerability profiles.

Methods: AIMS-CT1 is an international, multi-site, double-blind, parallel group Phase II randomized clinical trial. It will include 130 males and females aged 5:0–17:11 years, with a diagnosis of ASD and fluent speech. Eligible participants will be randomized on a ratio of 1:1 for a 16-week treatment period. Medication will be titrated over 5 weeks. The primary outcome is the effect on social function from weeks 0 to 16 measured on the Socialization domain of the Vineland Adaptive Behavior Scales, 3rd editionTM. Secondary outcome measures include the CGI–S (Clinical Global Impression–Severity), CGI–I (Clinical Global Impression–Improvement), other areas of adaptive function, social communication and other autism symptoms, co-occurring behavior problems and health-related quality of life. Genetic and electrophysiological markers will be examined as potential stratifiers for treatment response. Exploratory novel digital technologies will also be used to measure change, examining simultaneously the validity of digital biomarkers in natural environments. The safety and tolerability of the drug will also be examined. Our protocol is very closely aligned with a parallel Canadian trial of 90 participants (ARBA Study, US NCT number: NCT03887676) to allow for secondary combined analyses. Outcomes will be compared using both an Intent-to-reat and Per Protocol approach.

Discussion: The outcomes of this trial, combined with the parallel Canadian trial, will contribute to the evidence base for medications used to help social difficulties among young autistic individuals; demonstrate the capabilities of the AIMS-2-TRIALS network of academic centers to deliver clinical trials; and support future drug development.

Clinical Trial Registration: EudraCT number: 2018-000942-21 and ClinicalTrials.gov registry number: NCT03682978. Currently under protocol v.7.2, dated 20.11.2020.
Rationale for Mass Masking in Controlling the COVID-19 Pandemic
Authors: Tam Shing Yau , Tam Victor C. W. , Law Helen K. W. , Khaw May Ling , Lee Shara W. Y.
Published: August 2021

The rapid spread of the coronavirus disease 2019 (COVID-19) into a global pandemic caught the world unprepared. Previously effective measures for containing disease outbreaks were overwhelmed, necessitating strict controls such as lockdowns or curfews. Among the disease control interventions, community mass masking was one of the highly controversial issues with differing opinions on its indications or effectiveness from different health authorities around the world. Regions where community mass masking was timely introduced were associated with lower transmission rates, and more effective disease control. In this article, we discuss the evidence on the effectiveness, and rationale for community mass masking to prevent the COVID-19 transmission. Areas for further research to define the role of mass masking in light of the COVID-19 pandemic will be suggested. This would help policy makers in formulating mass masking policies.
Multiple Health Behavior Programs in School Settings: Strategies to Promote Transfer-of-Learning Through Life Skills Education
Authors: Velasco Veronica , Celata Corrado , Griffin Kenneth W. ,   Estensione LST group
Published: August 2021

Typically, schools implement health promotion programs that focus on a single behavioral domain. Multiple related health topics may be addressed using separate interventions, potentially producing overlap in program content. However, integrative approaches in health promotion have the potential to produce interventions capable of improving multiple health behaviors. In particular, more research is needed to identify the conditions and the factors that can promote the transfer of learning to broaden the target outcomes of health promotion programs. The present study aims to identify the characteristics of an evidence-based life skills education program that can facilitate the transfer of learning to different health behaviors not initially targeted by the program, and the strategies for achieving successful transfer. A two round Delphi method was used with a diverse group of 21 experts in health promotion, life skills education, and methods of pedagogy for early adolescent students. Questionnaires with open and closed-ended questions were administered on-line. Content qualitative analysis was run, integrating codes, subcategories, and categories of the two rounds of the study. Results showed strong consensus among experts about the potential for promoting the transfer of skills from one health domain to another. Many elements were identified as important factors that facilitate the transfer of learning. Strategies for successful transfer were related to teaching methods, educational approaches, and consistency with the broader school curriculum. Findings suggest that the successful transfer of learning to a new health domain requires that educators recognize its importance and explicitly designate it as an educational aim.
Blower-Powered Soft Inflatable Joints for Physical Human-Robot Interaction
Authors: Niiyama Ryuma , Seong Young ah , Kawahara Yoshihiro , Kuniyoshi Yasuo
Published: August 2021

Inflatables are safe and lightweight structures even at the human scale. Inflatable robots are expected to be applied to physical human-robot interaction (pHRI). Although active joint mechanisms are essential for developing inflatable robots, the existing mechanisms are complex in structure and it is difficult to integrate actuators, which diminish the advantages of inflatables. This study proposes blower-powered soft inflatable joints that are easy to fabricate and contain enough space for an actuation inside. The joints are driven by tendon wires pulled by linear actuators. We derived a theoretical model for both unilateral and bilateral joints and demonstrated a hugging robot with multiple joints as an application of the proposed joint mechanism. The novelty of the proposed joint mechanism and the inflatable robot is that rigid parts have been thoroughly eliminated and the tendons for actuation have been successfully hidden inside. Moreover, the active control of the internal pressure makes inflatables resistant to punctures. We expect that the contact safety of inflatable robots will facilitate advancement of the pHRI field.
Coping Mechanisms of Previously Diagnosed and New HIV-Discordant, Heterosexual Couples Enrolled in a Pilot HIV Self-Testing Intervention Trial in Central Uganda
Authors: Matovu Joseph K. B. , Kisa Rose , Malek Angela M. , Vrana-Diaz Caroline , Mukama Semei Christopher , Musoke William , Korte Jeffrey E. , Wanyenze Rhoda K.
Published: August 2021

Introduction: Learning that a couple has HIV-discordant results can create tensions in the relationship including separation. We explored the coping mechanisms of HIV-discordant, heterosexual couples enrolled in an HIV self-testing (HIVST) intervention trial in Central Uganda.

Materials and Methods: This qualitative study was nested within a pilot HIVST intervention trial targeting pregnant women and their male partners in central Uganda. In-depth interviews were conducted with 18 individuals from 13 HIV-discordant couples between July and September 2018; 18 months after the end of the main trial. Data were collected on the couples' initial reactions after learning about their HIV-discordant status, mechanisms adopted by couples to cope with HIV-discordance, and suggestions on how couples in similar situations can be supported. Interviews were transcribed verbatim and analysed manually following a thematic framework approach.

Findings: Of the 13 HIV-discordant couples, the female partner was HIV-positive (M–F+) in seven, while the male partner was HIV-positive (F–M+) in six. The mean (±SD) age of the participants was 32.6 (±6.4) years and participants had stayed together for an average of 5.5 (±3.6) years. Fourteen participants from nine couples already knew about their HIV-discordant status by the time they participated in the HIVST trial. After learning about their HIV-discordant status, most individuals (15) thought of abandoning their relationship; three (3) thought of committing suicide. To cope with HIV-discordance, some couples reported that they sought professional counselling support from healthcare providers, and this was particularly true for couples that were already aware of their HIV-discordant status by the time they participated in the HIVST trial. However, new couples that learnt about their HIV-discordant status after participating in the trial reported that they sought psycho-social support from friends or relatives. In the majority of cases, couples reported that they reduced the frequency of sex or abstained from sex. Some couples temporarily separated from their partners, while a few others resorted to using condoms to reduce HIV infection risk.

Conclusion: Couples used a variety of approaches to cope with HIV-discordance. Study findings underscore the importance of ongoing professional counselling and psycho-social support in helping couples to cope with HIV-discordance.
Social Relations and Everyday Consumption Rituals: Barriers or Prerequisites for Sustainability Transformation?
Authors: Boström Magnus
Published: August 2021

Macro-institutional structures and consumerist culture force and urge people to reproduce unsustainable levels of consumption. A crucial role for sociology, the article argues, is to address theoretically and empirically the intersection between social relations and (over)consumption. The purpose with this article is to address how social relations are involved in both reproducing and challenging consumer culture. This is done by emphasizing the intersection of consumer culture and socially integrating everyday rituals and drawing on literature on both voluntary and involuntary (the pandemic) disruption of consumer practices. The Covid-19 pandemic brings unexpected opportunities to highlight this intersection, as the pandemic offers a window of opportunity for lifestyle change. The review shows there are important lessons about both challenges and opportunities, gained from both voluntary and involuntary disruption of consumer practices.
Magnetospheric Mass Density as Determined by ULF Wave Analysis
Authors: Takahashi Kazue , Denton Richard E.
Published: August 2021

The technique to estimate the mass density in the magnetosphere using the physical properties of observed magnetohydrodynamic waves is known as magnetoseismology. This technique is important in magnetospheric research given the difficulty of determining the density using particle experiments. This paper presents a review of magnetoseismic studies based on satellite observations of standing Alfvén waves. The data sources for the studies include AMPTE/CCE, CRRES, GOES, Geotail, THEMIS, Van Allen Probes, and Arase. We describe data analysis and density modeling techniques, major results, and remaining issues in magnetoseismic research.
Constrained Dynamic Mean-Variance Portfolio Selection in Continuous-Time
Authors: Wu Weiping , Wu Lifen , Xue Ruobing , Pang Shan
Published: August 2021

This paper revisits the dynamic MV portfolio selection problem with cone constraints in continuous-time. We first reformulate our constrained MV portfolio selection model into a special constrained LQ optimal control model and develop the optimal portfolio policy of our model. In addition, we provide an alternative method to resolve this dynamic MV portfolio selection problem with cone constraints. More specifically, instead of solving the correspondent HJB equation directly, we develop the optimal solution for this problem by using the special properties of value function induced from its model structure, such as the monotonicity and convexity of value function. Finally, we provide an example to illustrate how to use our solution in real application. The illustrative example demonstrates that our dynamic MV portfolio policy dominates the static MV portfolio policy.
Seasonal Dynamics of Dry Matter Accumulation and Nutrients in a Mature Miscanthus × giganteus Stand in the Lower Silesia Region of Poland
Authors: Mos Michal , Robson Paul R. H. , Buckby Sam , Hastings Astley F. , Helios Waldemar , Jama-Rodzeńska Anna , Kotecki Andrzej , Kalembasa Dorota , Kalembasa Stanisław , Kozak Marcin , Chmura Kazimierz , Serafin-Andrzejewska Magdalena , Clifton-Brown John
Published: August 2021

Biomass from M. × giganteus has great promise for use within the bioeconomy sectors, but to maximise environmental benefits, crops must produce high yields while minimising energetically costly inputs. Complex interactions between soil conditions, climatic variations, plant maturity and genotype influence yields and nutrient dynamics, which in turn impacts crop sustainability. To investigate the flux of growth and nutrients in response to a changing environment, M. × giganteus was grown in southwest Poland and sampled monthly (June–November) from 2010 to 2012. Measurements examined the interaction between plant growth and leaf development, and nutrient (N, P, K, Ca and Mg) concentrations of rhizomes, stems and leaves. The three growth years studied were markedly different for growth and meteorological conditions. Between 2010 and 2011, above ground biomass yield increased significantly from 16.5 ± 0.4 t ha−1 to 20.1 ± 0.5 t ha−1. The 2012 rhizome weights at the beginning of the growth season were halved due to extreme frost; however, resulting yield was similar (19.9 ± 0.6 5 t ha−1). Final yield from all three years were successfully predicted using MISCANFOR, and modelling indicated crop yield was water-limited. The seasonal flux of N and K from rhizome to stems and leaves then back to the rhizome at the onset of senescence was as expected in 2010 and 2011. In 2012, no such trend was evident especially for N suggesting different macronutrient sources from rhizome and soil improves the resilience of perennial crop yield across a range of diverse growth conditions.
Climate Variability and Change Affect Crops Yield under Rainfed Conditions: A Case Study in Gedaref State, Sudan
Authors: Osman Maysoon A. A. , Onono Joshua Orungo , Olaka Lydia A. , Elhag Muna M. , Abdel-Rahman Elfatih M.
Published: August 2021

It is projected that, on average, annual temperature will increase between 2 °C to 6 °C under high emission scenarios by the end of the 21st century, with serious consequences in food and nutrition security, especially within semi-arid regions of sub-Saharan Africa. This study aimed to investigate the impact of historical long-term climate (temperature and rainfall) variables on the yield of five major crops viz., sorghum, sesame, cotton, sunflower, and millet in Gedaref state, Sudan over the last 35 years. Mann–Kendall trend analysis was used to determine the existing positive or negative trends in temperature and rainfall, while simple linear regression was used to assess trends in crop yield over time. The first difference approach was used to remove the effect of non-climatic factors on crop yield. On the other hand, the standardized anomaly index was calculated to assess the variability in both rainfall and temperature over the study period (i.e., 35 years). Correlation and multiple linear regression (MLR) analyses were employed to determine the relationships between climatic variables and crops yield. Similarly, a simple linear regression was used to determine the relationship between the length of the rainy season and crop yield. The results showed that the annual maximum temperature (Tmax) increased by 0.03 °C per year between the years 1984 and 2018, while the minimum temperature (Tmin) increased by 0.05 °C per year, leading to a narrow range in diurnal temperature (DTR). In contrast, annual rainfall fluctuated with no evidence of a significant (p > 0.05) increasing or decreasing trend. The yields for all selected crops were negatively correlated with Tmin, Tmax (r ranged between −0.09 and −0.76), and DTR (r ranged between −0.10 and −0.70). However, the annual rainfall had a strong positive correlation with yield of sorghum (r = 0.64), sesame (r = 0.58), and sunflower (r = 0.75). Furthermore, the results showed that a longer rainy season had significant (p < 0.05) direct relationships with the yield of most crops, while Tmax, Tmin, DTR, and amount of rainfall explained more than 50% of the variability in the yield of sorghum (R2 = 0.70), sunflower (R2 = 0.61), and millet (R2 = 0.54). Our results call for increased awareness among different stakeholders and policymakers on the impact of climate change on crop yield, and the need to upscale adaptation measures to mitigate the negative impacts of climate variability and change.
Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy
Authors: Morroni Gianluca , Fioriti Simona , Salari Federica , Brenciani Andrea , Brescini Lucia , Mingoia Marina , Giovanetti Eleonora , Pocognoli Antonella , Giacometti Andrea , Molinelli Elisa , Offidani Annamaria , Simonetti Oriana , Cirioni Oscar
Published: August 2021

Background: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guidelines. Non-susceptible isolates were epidemiologically characterized by pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing, and further investigated by PCR and whole genome sequencing to detect penicillin-binding protein (PBP) mutations as well as antibiotic resistance and virulence genes. Results: Fourteen isolates out of two hundred and thirty-nine (5.8%) were non-susceptible to ceftaroline (MIC > 1 mg/L), with differences between the EUCAST and CLSI interpretations. The characterized isolates belonged to seven different pulsotypes and three different clones (ST228/CC5-t041-SCCmecI, ST22/CC22-t18014-SCCmecIV, and ST22/CC22-t022-SCCmecIV), confirming a clonal diffusion of ceftaroline non-susceptible strains. Mutations in PBPs involved PBP2a for ST228-t041-SCCmecI strains and all the other PBPs for ST22-t18014-SCCmecIV and ST22-t022-SCCmecIV clones. All isolates harbored antibiotic resistance and virulence genes with a clonal distribution. Conclusion: Our study demonstrated that ceftaroline non-susceptibile isolates belonged not only to ST228 strains (the most widespread clone in Italy) but also to ST22, confirming the increasing role of these clones in hospital infections.
Therapeutic Potential of Polyphenols in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia
Authors: Novak Valentina , Rogelj Boris , Župunski Vera
Published: August 2021

Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are severe neurodegenerative disorders that belong to a common disease spectrum. The molecular and cellular aetiology of the spectrum is a highly complex encompassing dysfunction in many processes, including mitochondrial dysfunction and oxidative stress. There is a paucity of treatment options aside from therapies with subtle effects on the post diagnostic lifespan and symptom management. This presents great interest and necessity for the discovery and development of new compounds and therapies with beneficial effects on the disease. Polyphenols are secondary metabolites found in plant-based foods and are well known for their antioxidant activity. Recent research suggests that they also have a diverse array of neuroprotective functions that could lead to better treatments for neurodegenerative diseases. We present an overview of the effects of various polyphenols in cell line and animal models of ALS/FTD. Furthermore, possible mechanisms behind actions of the most researched compounds (resveratrol, curcumin and green tea catechins) are discussed.
Plastic Behavior of Laser-Deposited Inconel 718 Superalloy at High Strain Rate and Temperature
Authors: Peroni Lorenzo , Scapin Martina
Published: August 2021

Nickel-based superalloys have several applications for components exposed to high temperatures and high strain rate loading conditions during services. The objective of this study was to investigate the tensile properties of Inconel 718 produced using the laser metal deposition technique. Specimens with different heat treatments were investigated. Experimental tests were performed at the DYNLab at Politecnico di Torino (Italy). The temperature sensitivity was investigated between 20 °C and 1000 °C on a Hopkinson bar setup at a nominal strain rate of 1500 s−1. The specimens heating was obtained by means of an induction heating system, and the temperature control was performed by thermocouples, an infrared pyrometer, and a high-speed infrared camera. The thermal images were analyzed to check the uniformity of the heating and to investigate the presence of adiabatic self-heating. The results showed that the materials strength exhibited a significant drop starting from 800 °C. The strain rate influence was investigated at room temperature, and limited sensitivity was found covering six orders of magnitude in the strain rate. A preliminary analysis of the fracture mode was performed. Finally, different solutions for the strength material modeling were proposed and discussed with the aim of identifying models to be used in finite element simulations.
Influence of Yeast Strain on Odor-Active Compounds in Fiano Wine
Authors: Genovese Alessandro , Caporaso Nicola , Moio Luigi
Published: August 2021

The type of yeast strain used for wine alcoholic fermentation dramatically affects its final volatile composition and, therefore, its sensory properties. In this study, the influence of four oenological Saccharomyces strains (three S. cerevisiae and one S. bayanus) on wine volatile composition was determined on the Fiano variety, a typical cultivar from the Campania region (Italy), fermented in oak barrique. Fiano wines were analyzed by means of gas chromatography/mass spectrometry (GC/MS) and gas chromatography/olfactometry (GC/O). The results showed that the four selected yeast strains had a significant impact on the majority of volatile compounds as shown by the concentration of volatile compounds and based on the Aroma Extract Dilution Analysis (AEDA) values for many of the odor volatile compounds. This resulted in a dramatic change of the odor impact of the wines, such as the “fruity” attribute, which was higher compared to the control, and caused some changes of other odor attributes, particularly “floral”, “phenolic” and “honey”. This research demonstrates the potential of using these selected yeast strains and this technological approach of oak fermentation for this typical white wine grape variety.
Enumeration of Brettanomyces in Wine Using Impedance
Authors: van Wyk Sanelle , Silva Filipa
Published: August 2021

Brettanomyces bruxellensis is a wine spoilage concern in wineries around the world. In order to maintain wine quality during storage and ageing, it is imperative to control and monitor this yeast. Being a fastidious slow growing yeast, which requires 5 to 14 days of incubation for visible growth in agar plates, it is difficult to detect growth (colonies) by conventional agar plate count method. Yeast enumeration by impedance was investigated because previous research using other microorganisms has shown that it is potentially faster than plate counting. The relationship between plate counting and impedance detection times was investigated for Brettanomyces inoculated in red wine samples. A linear relationship between log plate count concentrations and impedance detection times was found. Incubation time was reduced from 120 h down to 0.9 and 57.7 h for samples with 6.7 × 107 and 1.8 × 102 cfu/mL, respectively, using the ‘indirect’ impedance method. The ‘direct’ method also reduced the incubation times to 9.5 and 81.9 h, for the same concentrations. The ‘indirect’ impedance method has the potential to be used by the wine industry to control and monitor the Brettanomyces numbers in wines.