bluuebunny/crossref_metadata_2025 huggingface dataset:
An Interdisciplinary Model to Foster Existential Resilience and Transformation
Authors: Stålbrand Ingela Steij , Brissman Ive , Nyman Lovisa , Sidenvall Erik , Tranberg Mattias , Wallin Anika , Wamsler Christine , Jacobsen Juliet
Published: January 2025

Existential threats, including threats to the self, society, and the planet, are present throughout the lifespan and form a core element of the human experience. To consolidate knowledge and constructs about how people can adequately respond to existential threats, we convened an interdisciplinary working group, which consisted of eight researchers from the fields of psychology, systemic theology, practical theology, religious studies, cognitive science, palliative care, and sustainability science. The group met one day per week for 9 months to engage in an interactive co-creative process of data collection and analyses, discussion, iterative presentations, and writing. The process resulted in the development of an interdisciplinary model that pulls together the key themes of how people, societies, and systems can foster existential resilience and transformation. The model consists of three axes across which we (individuals, groups, systems) have to strengthen or stretch our “inner muscles”: connectedness, agency, and time. All axes contribute to the development of our inner capacities and, ultimately, meaning and purpose, which are crucial to support resilience and transformation. Our interdisciplinary overarching model provides a common conceptualization for existential resilience and transformation that can bridge existing research to support individual, collective, and large-scale system-change work. Its relevance and potential implementation are illustrated and presented from different disciplinary angles.
Effect of Recovery Treatment on the Microstructure and Tribological Properties of Ultrasonic Impacted Al2FeCoNiCrW0.5 High-Entropy Alloy Coatings
Authors: Zhang Chong , Li Qingda , Hu Jun , Zhao Shengxue , Zheng Xin , Wang Hao , Liu Hongyuan
Published: January 2025

To investigate the effect of recovery treatment on the microstructure and tribological properties of ultrasonic impact-treated Al2FeCoNiCrW0.5 high-entropy alloy coatings, laser cladding technology was used to fabricate coatings on a G10450 steel substrate, followed by ultrasonic impact treatment (UIT) and recovery treatment (HR, 300 °C). The results showed that the Al2FeCoNiCrW0.5 high-entropy alloy coating consisted of BCC and FCC phases. Ultrasonic impact treatment slightly broadened the XRD diffraction peaks, while the recovery treatment had minimal effect on them. Ultrasonic impact also refined the coating grains. Ultrasonic impact treatment increased the coating hardness from 738 HV0.5 to 856 HV0.5. Although the subsequent post-annealing slightly reduced the hardness to 806 HV0.5, it significantly improved wear resistance, with wear loss decreasing from 3.273 mm3 to 2.881 mm3, representing a 15% reduction in wear rate. The improvement in wear resistance was attributed to a change in the wear mechanism of the high-entropy alloy coating. Before and after post-annealing, the mechanism transitioned from abrasive wear, adhesive wear, and oxidative wear to primarily abrasive wear and oxidative wear. Additionally, the recovery treatment transformed the surface from hard and brittle to ductile and resilient.
Mortality of Post-COVID-19 Condition: 2025 Update
Authors: Lippi Giuseppe , Sanchis-Gomar Fabian
Published: January 2025

Background: The coronavirus disease 2019 (COVID-19) pandemic has generated profound health, societal, and economic consequences, which have been further compounded by long-term sequelae commonly referred to as post-COVID-19 or long-COVID syndrome. Understanding the real-world impact of post-COVID-19 mortality is therefore critical for effective healthcare planning and resource allocation. Methods: A descriptive epidemiological study was conducted using data from the US National Center for Health Statistics 2018–2024 to identify deaths attributed to the post-COVID-19 condition, as classified by the ICD-10 code U09.9, from October 2021 to December 2024. Demographic factors such as gender, age, and place of death were also extracted. Results: By December 2024, 2653 US deaths were classified under the ICD-10 code U09.9, corresponding to an age-adjusted mortality rate of 0.089 × 100,000. Mortality was significantly higher in males compared to females (0.098 vs. 0.081 × 100,000; p < 0.001). A clear age-related mortality gradient was observed, with rates increasing almost linearly with advancing age. The largest fraction of post-COVID-19 deaths occurred at home (33.0%), followed by nursing homes (26.3%) and medical facilities (24.1%). Conclusions: These findings highlight the substantial yet complex impact of the post-COVID-19 condition on mortality, with higher rates observed in males, older adults, and individuals at home, highlighting the need for targeted healthcare interventions and resource allocation, particularly for these higher-risk groups.
Comments and Illustrations of the European Federation of Societies for Ultrasound in Medicine Guidelines: Benign Pleura Lesions (Benign Pleura Thickening, Lesions and Masses)—What Can Be Seen on Transthoracic Ultrasound?
Authors: Möller Kathleen , Maruskin Tomas , Ludwig Michael , Blank Wolfgang , Eisenmann Stephan , Jenssen Christian , Findeisen Hajo , Möller Burkhard , Dietrich Christoph F.
Published: January 2025

Pleural thickening can be the result of inflammation or infection but can also have a neoplastic origin. Depending on the clinical context, a pleural lesion or mass is often initially suspected of malignancy. Benign pleural tumors are rare, and their appearance on ultrasound (US) is also described less frequently than pleural metastases or malignancies. There are few descriptions of contrast-enhanced Ultrasound (CEUS) in particular. This review introduces the basics of transthoracic ultrasound (TUS) of the pleura and CEUS of the pleura and lung. CEUS is recommended for pulmonary applications in the EFSUMB guidelines in non-hepatic applications. This article provides an overview of the characteristics of benign pleural thickening, tumor-like lesions, and benign pleural tumors on transthoracic B-mode US with color Doppler imaging (CDI) and CEUS. In detail, characteristics in TUS and CEUS are described for infectious/inflammatory pleural thickening (empyema, tuberculous pleuritis, hemothorax, fibrothorax), pleural thickening in various systemic diseases, in tumor-like conditions (plaques, splenosis, endometriosis, mesothelial cysts, lymphangiomatosis) and benign tumors (lipoma, benign SFT, schwannoma, solitary extramedullary/extraosseous plasmacytoma). The descriptions are illustrated by corresponding US and CEUS images.
Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker
Authors: Hörauf Jason-Alexander , Singh Amit , Voth Maika , Moheimani Hamed , Schindler Cora Rebecca , Relja Borna , Leppik Liudmila , Marzi Ingo , Henrich Dirk
Published: January 2025

Background/Objectives: Liver injury is common after abdominal trauma. However, the established biomarkers of liver injury, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), lack accuracy. This study investigates whether specific liver-related microRNAs (miRNAs) are released into the circulation in trauma patients with liver injury and whether they can indicate liver damage in the early phase after major trauma. Methods: A retrospective analysis of prospectively collected data and blood samples from 26 trauma patients was conducted. The levels of miRNA-21-5p, -122-5p, -191-5p, -192-3p, and -212-3p were measured in patients with computed tomography-confirmed liver trauma (LT group, n = 12) and polytrauma patients without liver trauma (PT group, n = 14) upon emergency room (ER) admission, and 24 and 48 h after trauma. Additionally, liver-type fatty acid binding protein (L-FABP) was measured, as it has recently been discussed in the context of abdominal trauma. Results: Only miRNA-122-5p showed a significant increase in the LT group compared to the PT group, but only at the 48 h time point (p = 0.032). Conversely, L-FABP (p = 0.018) and ALT (p = 0.05) were significantly elevated in the LT group compared to the PT group at the time of ER admission. There was a moderate correlation between miRNA-122-5p and AISAbdomen (p = 0.056) and transfused red blood cell concentrates (p = 0.055). L-FABP correlated strongly with the ALT levels (p = 0.0009) and the length of stay in the ICU (p = 0.0086). Conclusions: In this study, the liver-specific miRNA-122-5p did not effectively indicate liver injury in the early acute post-traumatic phase. Future research with a large sample size should investigate whether other miRNAs can more accurately predict liver injury and the extent of hepatocellular injury, particularly in the acute post-traumatic phase.
Data from Emergency Medical Service Activities: A Novel Approach to Monitoring COVID-19 and Other Infectious Diseases
Authors: del Re Daniele , Palla Luigi , Meridiani Paolo , Soffi Livia , Loiudice Michele Tancredi , Antinozzi Martina , Cattaruzza Maria Sofia
Published: January 2025

Background: Italy, particularly the northern region of Lombardy, has experienced very high rates of COVID-19 cases and deaths. Several indicators, i.e., the number of new positive cases, deaths and hospitalizations, have been used to monitor virus spread, but all suffer from biases. The aim of this study was to evaluate an alternative data source from Emergency Medical Service (EMS) activities for COVID-19 monitoring. Methods: Calls to the emergency number (112) in Lombardy (years 2015–2022) were studied and their overlap with the COVID-19 pandemic, influenza and official mortality peaks were evaluated. Modeling it as a counting process, a specific cause contribution (i.e., COVID-19 symptoms, the “signal”) was identified and enucleated from all other contributions (the “background”), and the latter was subtracted from the total observed number of calls using statistical methods for excess event estimation. Results: A total of 6,094,502 records were analyzed and filtered for respiratory and cardiological symptoms to identify potential COVID-19 patients, yielding 742,852 relevant records. Results show that EMS data mirrored the time series of cases or deaths in Lombardy, with good agreement also being found with seasonal flu outbreaks. Conclusions: This novel approach, combined with a machine learning predictive approach, could be a powerful public health tool to signal the start of disease outbreaks and monitor the spread of infectious diseases.
Impact of HIF-1α, LOX and ITGA5 Synergistic Interaction in the Tumor Microenvironment on Colorectal Cancer Prognosis
Authors: Tatlı Doğan Hayriye , Doğan Mehmet , Kahraman Seda , Çanakçı Doğukan , Şendur Mehmet Ali Nahit , Tahtacı Mustafa , Erdoğan Fazlı
Published: January 2025

Background: As colorectal cancers are histopathologically and molecularly highly heterogeneous tumors, it is necessary to consider the tumor’s microenvironment as well as its cellular characteristics in order to determine the biological behavior of the tumor. This study included 100 patients who underwent resection for colorectal cancer. We aimed to investigate the relationships between the expression status of the HIF-1α, LOX and ITGA5 proteins and clinicopathologic parameters. Methods: HIF-1α, LOX and ITGA5 antibodies were applied immunohistochemically to tissue microarrays prepared from tumor samples. Expression status in the tumor microenvironment were evaluated using a combined scoring system based on staining intensity and the percentage of positively stained cells. Nuclear HIF-1α expression in tumor cells was quantified, with >1% considered positive. The staining of HIF-1α, ITGA5 and LOX was analyzed in relation to prognostic and molecular features. Results: The staining of HIF-1α, ITGA5 and LOX in the tumor microenvironment demonstrated a positive correlation with one another and with HIF-1α and LOX expression in tumor cells. In patients with KRAS, NRAS or BRAF mutation and the moderate to strong expression of all three of these proteins in the tumor microenvironment, the number of metastatic lymph nodes was higher than in other patients. Stage IV patients with the moderate to strong expression of HIF-1α, ITGA5 or LOX in the microenvironment had lower progression-free survival than those with weak expression (p < 0.05). In addition, female gender; moderate to strong HIF-1α, LOX and ITGA5 stromal expression; and metastatic first line chemotherapy only were found to be independently associated with an increased risk of progression. Conclusions: These markers may be useful in predicting treatment responses and may also guide the development of alternative or combined treatments that specifically target molecules such as HIF and LOX. Our study should be supported by more comprehensive studies addressing the tumor stroma and its prognostic importance.
Yaws in Africa: Past, Present and Future
Authors: Vicar Ezekiel K. , Simpson Shirley V. , Mensah Gloria I. , Addo Kennedy K. , Donkor Eric S.
Published: January 2025

Background: Yaws is an infectious, neglected tropical disease that affects the skin of many children and adolescents who live in poor, rural, low-income communities in humid, tropical areas of Africa, Southeast Asia, and the Pacific Islands. Yaws is currently endemic in at least 15 countries, but adequate surveillance data are lacking. In line with the WHO’s effort to improve early detection, diagnosis, and proper management leading to the eventual eradication of yaws, this article reviews the existing literature on yaws in Africa to highlight the epidemiological pattern, genetic variability, diagnosis modalities, treatment, and control strategies, the challenges and prospects for yaws eradication. Methods: We searched PubMed and Scopus databases to identify published data in line with the review objectives. Results: One hundred and eighty-eight peer-reviewed articles were identified by PubMed and Scopus, out of which thirty were eligible. The studies covered 11 African countries, with the reported prevalence ranging from 0.50% to 43.0%. Conclusions: There is a great prospect for eradication if countries capitalize on the availability of simple, inexpensive, and well-tolerated oral treatment that has proven effective, validated point-of-care diagnostic tests and new molecular tests. Countries should embark on integrated disease control efforts to increase sustainability and improve the quality of life for people living with this NTD in poor communities.
Conceptual Knowledge of Oral Health Among Primary School Teachers in Riyadh, Saudi Arabia—A Cross Sectional Survey
Authors: Khanagar Sanjeev. B. , Albar Rayan , Alghamdi Abdullah , Alshamrani Sultan , Alhussain Yousif
Published: January 2025

Background/Objectives: School teachers need to have a better understanding of oral health aspects as schools serve as an effective environment for learning. Educators hold a significant position in conveying the importance they attribute to oral health in their lives. According to the World Health Organization, school teachers should include oral health promotion activities to evaluate students’ oral health, track injuries, illnesses, and absenteeism related to oral health, advocate oral health prevention, and serve as role models. The aim of this study was to evaluate the conceptual understanding of oral health among primary school teachers in Riyadh, Saudi Arabia. Methods: Data for this study were collected from 404 primary school teachers using a structured and pre-validated comprehensive measure of oral health knowledge (CMOHK) questionnaire. Results: The distribution of CMOHK scores indicated that 247 (61.2%) participants demonstrated good conceptual knowledge, 95 (23.5%) participants exhibited fair knowledge, and 62 (15.3%) participants were classified as having poor knowledge. The male group exhibited lower values for the CMOHK score in comparison with the female group; however, these findings were not statistically significant. The findings indicated that the group of government school teachers exhibited higher values for the dependent variable CMOHK score compared to their private school counterparts. Conclusions: The average CMOHK score observed in this study is regarded as fair. It is crucial for school teachers to possess strong oral health knowledge, as they significantly influence the oral health knowledge and behaviors of children.
Outcomes of Broader Genomic Profiling in Metastatic Colorectal Cancer: A Portuguese Cohort Study
Authors: Roque Ricardo , Santos Rita , Santos Luís Guilherme , Coelho Rita , Fernandes Isabel , Cunha Gonçalo , Gonçalves Marta , Fraga Teresa , Paulo Judy , Bonito Nuno
Published: January 2025

Background: Colorectal cancer (CRC) is the third most diagnosed cancer globally and the second leading cause of cancer-related deaths. Despite advancements, metastatic CRC (mCRC) has a five-year survival rate below 20%. Next-generation sequencing (NGS) is recommended nowadays to guide mCRC treatment; however, its clinical utility when compared with traditional molecular testing in mCRC is debated due to limited survival improvement and cost-effectiveness concerns. Methods: This retrospective study included mCRC patients (≥18 years) treated at a single oncology centre who underwent NGS during treatment planning. Tumour samples were analysed using either a 52-gene Oncomine™ Focus Assay or a 500+-gene Oncomine™ Comprehensive Assay Plus. Variants were classified by clinical significance (ESMO ESCAT) and potential benefit (ESMO-MCBS and OncoKBTM). The Mann–Whitney and Chi square tests were used to compare characteristics of different groups, with significance at p < 0.05. Results: Eighty-six metastatic colorectal cancer (mCRC) patients were analysed, all being MMR proficient. Most cases (73.3%) underwent sequencing at diagnosis of metastatic disease, using primary tumour samples (74.4%) and a focused NGS assay (75.6%). A total of 206 somatic variants were detected in 86.0% of patients, 31.1% of which were classified as clinically significant, predominantly KRAS mutations (76.6%), with G12D and G12V variants as the most frequent. Among 33.7% RAS/BRAF wild-type patients, 65.5% received anti-EGFR therapies. Eleven patients (12.8%) had other actionable variants which were ESCAT level I-II, including four identified as TMB-high, four KRAS G12C, two BRAF V600E, and one HER2 amplification. Four received therapies classified as OncoKbTM level 1–2 and ESMO-MCBS score 4, leading to disease control in three cases. Conclusions: NGS enables the detection of rare variants, supports personalised treatments, and expands therapeutic options. As new drugs emerge and genomic data integration improves, NGS is poised to enhance real-world mCRC management.
Federated Learning and Reputation-Based Node Selection Scheme for Internet of Vehicles
Authors: Su Zhaoyu , Cheng Ruimin , Li Chunhai , Chen Mingfeng , Zhu Jiangnan , Long Yan
Published: January 2025

With the rapid development of in-vehicle communication technology, the Internet of Vehicles (IoV) is gradually becoming a core component of next-generation transportation networks. However, ensuring the activity and reliability of IoV nodes remains a critical challenge. The emergence of blockchain technology offers new solutions to the problem of node selection in IoV. Nevertheless, traditional blockchain networks may suffer from malicious nodes, which pose security threats and disrupt normal blockchain operations. To address the issues of low participation and security risks among IoV nodes, this paper proposes a federated learning (FL) scheme based on blockchain and reputation value changes. This scheme encourages active involvement in blockchain consensus and facilitates the selection of trustworthy and reliable IoV nodes. First, we avoid conflicts between computing power for training and consensus by constructing state-channel transitions to move training tasks off-chain. Task rewards are then distributed to participating miner nodes based on their contributions to the FL model. Second, a reputation mechanism is designed to measure the reliability of participating nodes in FL, and a Proof of Contribution Consensus (PoCC) algorithm is proposed to allocate node incentives and package blockchain transactions. Finally, experimental results demonstrate that the proposed incentive mechanism enhances node participation in training and successfully identifies trustworthy nodes.
Enhanced Fault Prediction for Synchronous Condensers Using LLM-Optimized Wavelet Packet Transformation
Authors: Zhang Dongqing , Li Shenglong , Hong Tao , Zhang Chaofeng , Zhao Wenqiang
Published: January 2025

This paper presents an enhanced fault prediction framework for synchronous condensers in UHVDC transmission systems, integrating Large Language Models (LLMs) with optimized Wavelet Packet Transform (WPT) for improved diagnostic accuracy. The framework innovatively employs LLMs to automatically optimize WPT parameters, addressing the limitations of traditional manual parameter selection methods. By incorporating a Multi-Head Attention Gated Recurrent Unit (MHA-GRU) network, the system achieves superior temporal feature learning and fault pattern recognition. Through intelligent parameter optimization and advanced feature extraction, the LLM component intelligently selects optimal wavelet decomposition levels and frequency bands, while the MHA-GRU network processes the extracted features for accurate fault classification. Experimental results on a high-capacity synchronous condenser demonstrate the framework’s effectiveness in detecting rotor, air-gap, and stator faults across diverse operational conditions. The system maintains efficient real-time processing capabilities while significantly reducing false alarm rates compared to conventional methods. This comprehensive approach to fault prediction and diagnosis represents a significant advancement in synchronous condenser fault prediction, offering improved accuracy, reduced processing time, and enhanced reliability for UHVDC transmission system maintenance.
Application of Convolutional Neural Networks and Recurrent Neural Networks in Food Safety
Authors: Ding Haohan , Hou Haoke , Wang Long , Cui Xiaohui , Yu Wei , Wilson David I.
Published: January 2025

This review explores the application of convolutional neural networks (CNNs) and recurrent neural networks (RNNs) in food safety detection and risk prediction. This paper highlights the advantages of CNNs in image processing and feature recognition, as well as the powerful capabilities of RNNs (especially their variant LSTM) in time series data modeling. This paper also makes a comparative analysis in many aspects: Firstly, the advantages and disadvantages of traditional food safety detection and risk prediction methods are compared with deep learning technologies such as CNNs and RNNs. Secondly, the similarities and differences between CNNs and fully connected neural networks in processing image data are analyzed. Furthermore, the advantages and disadvantages of RNNs and traditional statistical modeling methods in processing time series data are discussed. Finally, the application directions of CNNs in food safety detection and RNNs in food safety risk prediction are compared. This paper also discusses combining these deep learning models with technologies such as the Internet of Things (IoT), blockchain, and federated learning to improve the accuracy and efficiency of food safety detection and risk warning. Finally, this paper mentions the limitations of RNNs and CNNs in the field of food safety, as well as the challenges in the interpretability of the model, and suggests the use of interpretable artificial intelligence (XAI) technology to improve the transparency of the model.
Nutritional, Chemical, Antioxidant and Antibacterial Screening of Astragalus cicer L. and Astragalus glycyphyllos L. Different Morphological Parts
Authors: Shahrivari-Baviloliaei Saba , Konopacka Agnieszka , Pascoalino Liege Aguiar , Reis Filipa , Kunkowski Dawid , Petropoulos Spyridon A. , Konieczynski Pawel , Orhan Ilkay Erdogan , Plenis Alina , Viapiana Agnieszka
Published: January 2025

The chemical composition and biological activity of A. glycyphylos and A. cicer are scarcely investigated. In this study, the nutritional and chemical profiles of A. cicer and A. glycyphyllos, considering their different morphological parts (leaves, fruits and roots), were assessed together with their antioxidant and antibacterial potential. Our results showed that carbohydrates are the major macronutrients in both Astragalus species (above 62 g/100 g dry weight—DW). High amounts of ash (above 4.6 g/100 g DW) and protein (above 13.0 g/100 g DW) were also identified, particularly in leaves and fruits of A. cicer and A. glycyphyllos. Moreover, A. cicer was richer in sugars than A. glycyphyllos, while roots of both Astragalus species were the richest of fatty acids. Ten phenolic compounds were identified, with gallic acid and quercetin being predominant, above 49.84 and 37.27 μg/g DW, respectively. The mineral analysis revealed zinc and iron as the major constituents. Regarding the plants’ antioxidant and antibacterial activity, both Astragalus species had antioxidant potential, and their water extracts showed antibacterial activity against S. aureus and E. coli. Altogether, these results provide insight into the potential of A. glycyphyllos and A. cicer as a source of nutritional benefits and active phytochemicals for many people, and they can be applied in the food sector as foods and as promising sources of natural ingredients.
Predicting the Anti-SARS-CoV-2 Potential of Isoquinoline Alkaloids from Brazilian Siparunaceae Species Using Chemometric Tools
Authors: Gomes Brendo Araujo , Fernandes Diégina Araújo , Mendonça Simony Carvalho , Campos Mariana Freire , da Fonseca Thamirys Silva , Constant Larissa Esteves Carvalho , de Sousa Natalia Ferreira , Priscila Barros de Menezes Renata , de Oliveira Beatriz Albuquerque Custódio , da Silva Costa Stephany , Frensel Giovanna Barbosa , Rosa Alice Santos , Oliveira Thamara Kelcya Fonseca , Tucci Amanda Resende , Lima Júlia Nilo Henrique , Ferreira Vivian Neuza Santos , Miranda Milene Dias , Allonso Diego , Scotti Marcus Tullius , Leitão Suzana Guimarães , Leitão Gilda Guimarães
Published: January 2025

The COVID-19 pandemic has caused over 7 million deaths globally in the past four years. Siparuna spp. (Siparunaceae), which is used in Brazilian folk medicine, is considered a genus with potential antiviral alternatives. This study explored the correlation between phytochemicals in Siparuna leaf extracts (S. ficoides, S. decipiens, S. glycycarpa, S. reginae, and S. cymosa) and their potential against various SARS-CoV-2 targets. In vitro assays examined interactions between the spike protein and the ACE2 receptor, protease activity, and viral replication inhibition in Calu-3 cell models. UHPLC-MS/MS analysis, processed with MZmine and evaluated chemometrically, revealed isoquinoline alkaloids with bulbocapnine, showing promising therapeutic potential. Predictions regarding absorption, distribution, metabolism, excretion, and toxicity were conducted, along with molecular docking and dynamics simulations, to evaluate protein−ligand interaction stability. The results confirmed the antiviral activity of the Siparuna genus against SARS-CoV-2 targets, with 92% of the extracts maintaining over 70% cellular viability at 200 μg·mL−1 and 80% achieving more than 50% viral activity suppression at 50 μg·mL−1. These findings highlight the potential of isoquinoline alkaloids as novel anti-coronavirus agents and support the need for further exploration, isolation, and testing of Siparuna compounds in the fight against COVID-19.
Integrated Genetic Diversity and Multi-Omics Analysis of Colour Formation in Safflower
Authors: Qin Yonghua , Fan Kangjun , Yimamu Aidiya , Zhan Peng , Lv Lu , Li Gang , Liu Jiao , Hu Zunhong , Yan Xingchu , Hu Xueli , Liu Hong , Qin Rui
Published: January 2025

Safflower (Carthamus tinctorius L.) is a medicinal and edible cash crop that is widely cultivated worldwide. However, the genetic diversity of safflower germplasm resources and the reasons for the variations in safflower flower colour remain unclear. In this study, we used a combination of agronomic traits and Indel markers to assess the genetic diversity of 614 safflower germplasm resources. The results showed that most of the evaluated agronomic traits had high variability. The mean values of the Shannon’s information index (I) and polymorphism information content (PIC) in 50 pairs of Indel markers were 0.551 and 0.296, respectively. The population structure, neighbour-joining phylogeny, and principal coordinate analyses classified all genotypes into four subgroups, and 214 safflower core germplasms were constructed. Multiple analyses of genetic diversity parameters, range conformity, and the percentage of variance difference showed that the core germplasm did not differ significantly and could represent the original germplasm better. Transcriptome and metabolome analyses revealed that flavonoid synthesis-related genes, including CHS, F3H, ANS, and BZ1, were differentially expressed in different coloured safflowers. Most significantly, different genes and metabolite compounds in white safflowers were enriched upstream from the phenylpropanoid metabolic pathway to the production of naringenin, whereas those in red safflowers were concentrated in the downstream pathway from eriodictyol. Meanwhile, the preliminary quantification of anthocyanins and carotenoids extracted from red, orange, and white types of safflower showed that the level of both anthocyanins and carotenoids were highest in red types. This work provides new insights into the formation of different safflower flower colours and in the conservation and management of safflower germplasm.
The Cell Polarity Protein MPP5/PALS1 Controls the Subcellular Localization of the Oncogenes YAP and TAZ in Liver Cancer
Authors: Tóth Marcell , Wan Shan , Schmitt Jennifer , Birner Patrizia , Wei Teng , von Bubnoff Fabian , de la Torre Carolina , Thomann Stefan , Pinna Federico , Schirmacher Peter , Weiler Sofia Maria Elisabeth , Breuhahn Kai
Published: January 2025

The oncogenes yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are potent liver oncogenes. Because gene mutations cannot fully explain their nuclear enrichment, we aim to understand which mechanisms cause YAP/TAZ activation in liver cancer cells. The combination of proteomics and functional screening identified numerous apical cell polarity complex proteins interacting with YAP and TAZ. Co-immunoprecipitation (Co-IP) experiments confirmed that membrane protein palmitoylated 5 (MPP5; synonym: PALS1) physically interacts with YAP and TAZ. After removing different MPP5 protein domains, Co-IP analyses revealed that the PDZ domain plays a crucial role in YAP binding. The interaction between YAP and MPP5 in the cytoplasm of cancer cells was demonstrated by proximity ligation assays (PLAs). In human hepatocellular carcinoma (HCC) tissues, a reduction in apical MPP5 expression was observed, correlating with the nuclear accumulation of YAP and TAZ. Expression data analysis illustrated that MPP5 is inversely associated with YAP/TAZ target gene signatures in human HCCs. Low MPP5 levels define an HCC patient group with a poor clinical outcome. In summary, MPP5 facilitates the nuclear exclusion of YAP and TAZ in liver cancer. This qualifies MPP5 as a potential tumor-suppressor gene and explains how changes in cell polarity can foster tumorigenesis.
Tinostamustine (EDO-S101) and Its Combination with Celecoxib or Temozolomide as a Therapeutic Option for Adult-Type Diffuse Gliomas
Authors: Pawlak Wiktoria , Majchrzak-Celińska Aleksandra
Published: January 2025

Adult-type diffuse gliomas are characterized by inevitable recurrence and very poor prognosis. Novel treatment options, including multimodal drugs or effective drug combinations, are therefore eagerly awaited. Tinostamustine is an alkylating and histone deacetylase inhibiting molecule with great potential in cancer treatment. Thus, the aim of this study was to investigate its effects on glioma cells. In this context, tinostamustine was evaluated in monotherapy and as a combination therapy, with either celecoxib or temozolomide; additionally, the results were compared to the golden glioma chemotherapy standard—temozolomide. Our experiments, conducted on both temozolomide-sensitive U-87 MG astrocytoma and temozolomide-resistant U-138 MG glioblastoma cells revealed that tinostamustine and its combination with either celecoxib or temozolomide exert dose-dependent cytotoxicity, cause cell cycle arrest, induce oxidative stress-mediated apoptosis of malignant glioma cells, and mitigate their migratory potential. Astrocytoma cells were more susceptible to the tested treatments than glioblastoma cells, and, generally, those dual therapies were superior in anti-glioma efficacy compared to temozolomide. Overall, our study provides evidence that tinostamustine and the combination therapies consisting of tinostamustine and celecoxib or tinostamustine and temozolomide may represent a new approach for the effective treatment of malignant gliomas.
Energy Metabolism and Stemness and the Role of Lauric Acid in Reversing 5-Fluorouracil Resistance in Colorectal Cancer Cells
Authors: Fujiwara-Tani Rina , Luo Yi , Ogata Ruiko , Fujii Kiyomu , Sasaki Takamitsu , Sasaki Rika , Nishiguchi Yukiko , Mori Shiori , Ohmori Hitoshi , Kuniyasu Hiroki
Published: January 2025

While 5-fluorouracil (5FU) plays a central role in chemotherapy for colorectal cancer (CRC), resistance to 5FU remains a major challenge in CRC treatment, and its underlying mechanisms remain unclear. In this study, we investigated the relationship between 5FU resistance acquisition, stemness, and energy metabolism. Among the two CRC cell lines, HT29 cells exhibited glycolytic and quiescent properties, while CT26 cells relied on oxidative phosphorylation (OXPHOS) for energy. In contrast, the 5FU-resistant sublines (HT29R and CT26R), developed through continuous exposure to low concentrations of 5FU, demonstrated enhanced stemness. This was associated with glycolytic dominance, low proliferation, and reduced reactive oxygen species (ROS) production. However, treatment with the medium-chain fatty acid lauric acid shifted the cells to OXPHOS, reducing stemness, increasing ROS levels, and inducing cell death, therefore reversing 5FU resistance. These findings suggest that an enhancement in stemness and the reprogramming of energy metabolism play key roles in acquiring 5FU resistance in CRC. While lauric acid reversed 5FU resistance, further clinical studies are required.
The Training and Evaluation of the “Dual-Index” Screening Method for Neonatal Congenital Heart Disease: A Multi-Center Study in China
Authors: Huang Panpan , Gu Qing , Zhu Xiaoting , Haq Ijaz ul , Li Liling , Hu Xiaojing , Huang Guoying
Published: January 2025

Background: This study aimed to enhance the scope of neonatal congenital heart disease (CHD) screening by evaluating the effectiveness of training personnel in CHD screening using the “dual-index” method, combining pulse oximetry with cardiac murmur auscultation. Methods: From 2019 to 2022, a total of 2374 screening personnel from the Xinjiang, Yunnan, Hainan, Fujian, and Anhui provinces underwent training in neonatal CHD screening using the “dual-index” method, which involves pulse oximetry and cardiac murmur auscultation. Pre- and post-training assessments were conducted using a neonatal CHD screening knowledge questionnaire, distributed through the Questionnaire Star platform, to evaluate the impact of the training. The annual neonatal CHD screening rates were consistently recorded in these five provinces during the same period to assess the increase in screening coverage. Results: After the training, the screening personnel exhibited a significantly improved understanding of the neonatal CHD screening method (p < 0.001). Additionally, the professional background (t = −8.007, p < 0.001) and years of experience (t = 2.839, p = 0.005) of the screening personnel were identified as independent factors influencing their screening knowledge. During the same period, there was consistent linear growth in the screening coverage rate for neonatal CHD across the five provinces (χ2 = 121065.416, p < 0.001). Conclusion: Standardized training in the “dual-index” method, incorporating pulse oximetry and cardiac murmur auscultation, for screening personnel significantly enhances their screening knowledge, thereby playing a critical role in expanding the coverage of neonatal CHD screening.
Preoperative Non-Invasive Mapping for Targeted Concomitant Surgical Ablation of Non-Paroxysmal Atrial Fibrillation (PreMap Study)
Authors: Santer David , Gahl Brigitta , Dogan Ali , Bruehlmeier Florian , Camponovo Ulisse , Maguire Rory , Goldiger Larissa , Boss Vanessa , Weber Nicole , Schmuelling Lena , Gherca Stefan , Bremerich Jens , Cueni Nadine , Koechlin Luca , Kühne Michael , Miazza Jules , Reuthebuch Oliver , Hollinger Alexa , Siegemund Martin , Sticherling Christian , Eckstein Friedrich , Amacher Simon A.
Published: January 2025

Background/Objectives: The present study introduces our targeted approach for concomitant surgical ablation (CSA) using non-invasive phase mapping (NIPM) and describes its effectiveness regarding freedom from atrial fibrillation (AF). Methods: This retrospective study included cardiac surgical patients undergoing preoperative NIPM for CSA guidance. The primary outcome was freedom from AF six months after surgery. Key secondary outcomes were freedom from AF at hospital discharge and three months, frequency of biatrial ablation, feasibility and safety, the rate of CSA, complications, and levels of biomarkers. The control group consisted of patients undergoing CSA without NIPM. Results: Forty-four patients (Control: n = 31/NIPM: n = 13) were included. The NIPM group was younger (64 vs. 71 years [p = 0.044]), had a lower EuroSCORE II (2.6 vs. 3.4 [p = 0.041]), and a smaller left atrial size (46 mm vs. 54 mm [p = 0.025]). Surgery duration was longer in the NIPM group (285 vs. 230 min [p = 0.037]) with similar aortic cross-clamp times. Preoperative NIPM resulted in an effective frequency of CSA of 93%. CSA was more extensive in the NIPM group, with biatrial ablation performed in 54% vs. 26% of patients (p = 0.09). Conclusions: Routine preoperative NIPM in patients with non-paroxysmal atrial fibrillation might aid in increasing the number of patients receiving concomitant surgical ablation and developing a personalized CSA approach for every patient.
Assessment of Fluid Responsiveness via Central Venous Ultrasound Measurement: A Network Meta-Analysis
Authors: Berikashvili Levan B. , Kuznetsov Ivan V. , Yadgarov Mikhail Ya. , Ryzhkov Pavel V. , Polyakov Petr A. , Yavorovskiy Andrey G. , Yakovlev Alexey A. , Grechko Andrey V. , Likhvantsev Valery V.
Published: January 2025

Background: Ultrasonographic assessment of the diameters of various veins and their indices are among the most applied diagnostic tools for evaluating fluid responsiveness in clinical practice. Despite their widespread use, there is no definitive answer on which is preferable. Our study aimed to investigate the diagnostic accuracy of different venous diameters and their indices to assess fluid responsiveness. Methods: We conducted a systematic review and network meta-analysis, analyzing prospective studies evaluating the diagnostic accuracy of venous diameters (inferior vena cava [IVC], internal jugular vein [IJV], superior vena cava, and subclavian vena) and their indices for fluid responsiveness. Electronic databases were searched from inception until March 2024; this search was supplemented by snowballing methods. The risk of bias was evaluated with QUADAS-2, and evidence certainty was assessed using the GRADE approach. Nine prospective cohort studies (560 patients) were included. Results: The network meta-analysis revealed that the ΔCaval index exhibited a significant performance advantage over other “venous” test parameters. The caval index significantly outperformed IJV min/max and IVCmax. IJV index and IVCmin significantly outperformed IJVmin/max. The caval index was comparable to the IJV index. The caval index was comparable during mechanical ventilation and spontaneous breathing. Conclusions: In this meta-analysis, the ΔCaval index test showed higher diagnostic accuracy for fluid responsiveness compared with other venous tests. Caval and jugular indices displayed similar accuracy, and caval indices were consistent under mechanical ventilation and spontaneous breathing. Indices generally outperformed absolute values, except for IVCmin, which equaled the caval index in efficacy. This study was registered on the International Platform for Registered Protocols for Systematic Reviews and Meta-Analyses: INPLASY202430104.
Early and Mid-Term Outcomes of Isolated Type 2 Endoleak Refractory to an Embolization Procedure
Authors: Miceli Francesca , Dajci Ada , Di Girolamo Alessia , Nardis Piergiorgio , Ascione Marta , Cangiano Rocco , Gattuso Roberto , Sterpetti Antonio , di Marzo Luca , Mansour Wassim
Published: January 2025

Introduction: A type 2 endoleak (EL2) remains the most prevalent complication of endovascular aortic repair (EVAR) for an abdominal aortic aneurysm (AAA). Methods: We conducted a retrospective, single-center analysis, including patients who underwent embolization for an isolated EL2 after EVAR. The study population was stratified into two groups: Group A, consisting of patients whose EL2 resolved after the first embolization procedure, and Group B, consisting of those with refractory EL2 (rEL2). The indication for EL2 treatment was aneurysmal sac growth amounting to >10 mm from the index EVAR. The indications for endograft explantation were the absence of high comorbidities and persisting aneurysmal sac expansion. Those with high comorbidities were subjected to another endovascular procedure or a conservative approach, the latter being preferred. The primary endpoint was EL2 resolution. The secondary endpoints were mid-term outcomes in terms of aneurysmal sac shrinkage, stability and expansion rates, and aneurysm-related complications. Results: Among 57 patients, 19 patients (33.3%) showed signs of EL2 after the first embolization, whereas 38 (66.6%) presented rEL2. Of these, 14 (36.8%) presented significant aneurysmal sac expansion: 8 patients underwent a secondary embolization, while an open conversion was performed in the remaining 6 patients (42.8%), 4 of whom, in an elective setting, showed a complete resolution of EL2, while 2 patients treated in an urgent setting died from a ruptured aneurysm. Among the patients treated with a secondary embolization, only 2 patients presented EL2 resolution, while the other 6 patients (75%) showed rEL2. Out of the 38 patients with rEL2, 24 patients did not undergo further interventions; of these, 11 (45.8%) presented sac expansion, and 16% developed type IA EL. Conclusions: A strict follow-up and possibly a more aggressive treatment should be considered in an elective setting for patients with rEL2.
Post COVID-19 and Long COVID Symptoms in Otorhinolaryngology—A Narrative Review
Authors: Guntinas-Lichius Orlando , Bitter Thomas , Takes Robert , Lee Victor H. F. , Saba Nabil F. , Mäkitie Antti A. , Kowalski Luiz P. , Nixon Iain J. , Ferlito Alfio
Published: January 2025

Post/Long COVID (syndrome) is defined as a condition with symptoms persisting for more than 12 weeks after the onset of SARS-CoV-2 infection that cannot be explained otherwise. The prevalence of self-reported otorhinolaryngological Post/Long COVID symptoms is high. The aim of this review was to analyze the current literature regarding the actual prevalence, knowledge of the etiopathology, and evidence-based treatment recommendations of otorhinolaryngology-related Post/Long COVID symptoms. A systematic literature search of articles published since 2019 in PubMed and ScienceDirect was performed and resulted in 108 articles. These were the basis for this review and formed a comprehensive series of consented therapy statements on the most important of otorhinolaryngology-related Post/Long COVID symptoms. Otorhinolaryngological symptoms did not appear isolated but as part of a multi-organ syndrome. Self-reported otorhinolaryngology-related Post/Long COVID symptoms were often not confirmed by objective testing. The confirmed prevalence estimated for anosmia, dysgeusia, cough, facial palsy, hoarseness/dysphonia, acute hearing loss, tinnitus, and vertigo/dizziness was about 4%, 2%, 4–19%, 0%, 17–20%, 8%, 20%, and 5–26%, respectively. There are manifold theoretical concepts of the etiopathology of different symptoms, but there is no clear evidence-based proof. This certainly contributes to the fact that there is no effective specific treatment option for any of the symptoms mentioned. Healthcare pathways must be established so that otorhinolaryngological Post/Long COVID symptoms can be recognized and evaluated and otorhinolaryngologists can provide counseling. This would also help to establish and selectively include patients in clinical trials investigating specific therapeutic concepts.
Evaluation of Mechanical Properties of Sabai Grass (Eulaliopsis binata) Fibers and Epoxy Resin Composite Laminates Using Fly Ash as Filler Material
Authors: Kumar Shambhu , Das Ratnakar , Parida Sambit Kumar
Published: January 2025

The integration of sabai grass fibers and fly ash in epoxy resin combines the strengths of both materials for developing a tailor-made composite laminate that balances performance, sustainability, and cost-efficiency. This innovative blend of natural fibers and industrial waste promotes environmental conservation. The laminates produced could also be used in diverse industrial and structural applications. This study investigated the mechanical properties of composite laminates reinforced with sabai grass fibers, fly ash filler, and epoxy resin as the matrix. In this work, the hand lay-up method was used to fabricate composites with two stacking configurations ((0°/0°/0°/0°) and (0°/90°/90°/0°)) and filler contents of 1.5 wt.%, 3 wt.%, and 5 wt.%. Various weight fractions of fly ash filler and sabai grass fiber were integrated into the epoxy resin to evaluate their impact on tensile strength, flexural strength, and hardness. The experimental results indicate that adding fly ash significantly improves the composite’s hardness to 27 HV in the composites containing 5 wt.% filler, while sabai grass fibers contribute to enhanced tensile strength and flexural strength. The composites with (0°/0°/0°/0°) fibers and 5 wt.% filler showed a higher tensile strength of 63.5 MPa and flexural strength of 118.5 MPa. The fractured sample was analyzed with the help of FESEM images. The XRD analysis confirmed the presence of fly ash components suitable for forming a bond with epoxy. EDX was conducted to determine the elemental composition of the fly ash. FTIR analysis verified the removal of impurities such as dust, dirt, and lignin from the fiber surface following NaOH treatment.
Five New Species of Pezizales from Northeastern China
Authors: Chen Zhengqing , Bau Tolgor
Published: January 2025

Species belonging to the Pezizales are mainly saprobes in nature. They are most commonly observed in woodlands and humid environments. As a result of recent research conducted on the distribution of species in sandy areas and some National Forests Parks, five new species belonging to three genera were identified. A total of five species of disk fungi from Northeast China were identified and described based on morphological classification and molecular phylogenetics. These included Pulvinula (Pulvinula elsenensis, Pulvinula sublaeterubra), Microstoma (Microstoma jilinense, Microstoma changchunense), and Sarcoscypha (Sarcoscypha hongshiensis). Maximum likelihood and Bayesian analyses were performed using a combined nuc rDNA internal transcribed spacer region (ITS) and nuc 28S rDNA (nrLSU) dataset for the construction of phylogenetic trees. Morphological descriptions, line illustrations, and photographs of the ascocarps of these new species are provided, along with lists of the salient attributes exhibited by the species in the three genera under consideration.