bluuebunny/crossref_metadata_2025 huggingface dataset:
Identifikasi Makna Kafa’ah Dalam Perkawinan
Authors: Aziz Nasaiy , Junaidi Burmawi
Published: March 2023

Kafa'ah is one of the important things in marriage, this is intended to produce harmony in the household so that it can be nurtured and the creation of a happy and harmonious household. Islam provides guidelines for choosing a partner based on four important criteria, namely religion, wealth, descent, and beauty. The different views were expressed by community leaders in Gampong Lada Village, namely expanding the meaning of kafa'ah in marriage by adding educational, position and job criteria in interpreting the meaning of kafa'ah. The problem in this thesis is how the meaning and criteria of kafa'ah in marriage and how the community leaders of Gampong Lada interpret kafa'ah and its criteria in marriage. The type of research used by the author in compiling this thesis is descriptive qualitative research. The results of this study indicate that the community leaders of Gampong Lada Village are very understanding about the meaning of kafa'ah in marriage, because the community leaders of Gampong Lada are very knowledgeable about religious knowledge. Gampong Lada community leaders interpret kafa'ah as equality, equivalence, and harmony between the prospective groom and the prospective bride when they want to get married. And the community leaders of Gampong Lada are of the view that for now the aspects of property, education, work, and position are very relevant in terms of kafa'ah, but even so, community leaders in Gampong Lada Village, Mutiara Timur District, and Pidie Regency do not rule out the religious aspect of the kafa issue. At this point, the community leaders of Gampong Pepper view that the religious aspect as the most important aspect of the meaning of kafa'ah in marriage.
Mycofloral Pattern and Its Insights of Bioactive Compounds Against Pathogens
Authors: Rajathi S. , Murugesan S. , Babu S. , Shijila Rani A.S. , Ambikapathy V. , Prakash P. , Panneerselvam A.
Published: March 2023

Soil is considered a well-studied ecological niche for microorganisms that produce beneficial physiologically active natural compounds suited for medicinal purposes. The current study sought to elucidate antifungal activity and metabolism from fungi. The paddy field mycofloral diversity pattern was analyzed from the soil, and its physiochemical characteristics were also determined from the Kanyakumari district, Tamil Nadu, from 2016-2017. Totally twenty-seven fungal species were isolated from the paddy field soil. The physicochemical parameters of temperature, moisture content, pH, organic carbon, organic matter, and organic nitrogen were analyzed with significantly correlated. The most dominant fungal species include Aspergillus flavipes, A.flavus, A.fumigatus, A.nidulans, A.niger, A.ochraceous, Curvularia lunata, Fusarium solani, Saccharomyces cerevisiae, and Trichoderma viride were identified. Natural Products (NPs) synergistic effect was exploited to discover pairwise combinations with potential antifungal activity. A high-throughput screening approach with yeast revealed that NPs in molecules are the most promising novel synergies, namely, EUG+BER. This combination synergistically inhibits fungi, including human and crop pathogens such as Candida albicans, Aspergillus fumigatus, Zymoseptoria tritici, and Botrytis cinerea. This study was about screening pairwise NPs interactions as a tool to find novel antifungal synergies with the potential. Also, improved specificity may help manage fungal pathogens.
Zinc Oxide Nanoparticles as The Anti-Bacterial Tool: In Vitro Study
Authors: Satpathy Gargibala , Rebecca Jayanti , Sahu Umabati , Das Ipsita , Manikandan E.
Published: March 2023

A new class of metal oxide nanoparticles has recently been found to be widely used in various health-related research. In particular, Zinc Oxide (ZnO) nanoparticles have occupied a prominent status due to their unique chemical and electrical properties. In our study, we aim to reveal the anti-bacterial effect of these nanoparticles. The antibacterial activity of zinc oxide (ZnO) nanoparticles against isolated pathogenic Escherichia coli (strain MTCC 723) was determined from our study. The antimicrobial properties of ZnO were also determined by adjusting the concentration of ZnO nanoparticles. By sonicating ZnO nanoparticles in water, a homogenized suspension was created, and the infusion was made at 50 – 150g/ml concentrations. Anaerobic conditions were used to culture the pathogenic strain of microbial species, and DNA was extracted using an extraction kit. For testing, equalized standard dilutions of cultivated bacteria were utilized. The anti-bacterial capabilities of zinc oxide (ZnO) nanoparticles against bacteria were measured using spectroscopic and diffusion experiments. ZnO nanoparticles with a diameter of 50 nm and a concentration of 150 g/ml lysed Escherichia coli DNA and cells. According to the findings, ZnO nanoparticles with a concentration of 150 g/ml had significantly more vigorous activity against Escherichia coli. The agar diffusion method was used to quantify the anti-bacterial activity of zinc oxide (ZnO) and the quality and amount of DNA extraction in the presence of ZnO nanoparticles (ZnO). The pathogenic Escherichia coli cells and the gene DNA are killed at the optimal dose. This study aims to know the anti-bacterial properties of ZnO nanoparticles against pathogenic Escherichia coli cells. The optimization of ZnO nanoparticles concentration against its effect on Escherichia coli cells is the next aim of this study.
Long-Lasting Chemiluminescence by Aggregation-Induced Emission Surfactant with Ultralow Critical Micelle Concentration
Authors: Yu Youkai , Yuan Zhiqin , Lu Chao
Published: March 2023

The development of green and simple chemiluminescence (CL) systems with
intensive and long-lasting emission is highly desirable in lighting and
extension of their applications. In this study, it is found that the
involvement of aggregation-induced emission (AIE) surfactant could
greatly enhance the CL of luminol–H2O2–Co2+ system. The inserted
hydrophobic tetraphenylethylene fluorophore in AIE is able to increase
the hydrophobicity of alkyl chain and decrease the critical micelle
concentration (CMC) of surfactant. The synergistic effect of
micelle-improved enrichment and CL resonance energy transfer endows
luminol–H2O2–Co2+ system intensive and long-lasting emission under
neutral pH conditions (pH 7.4). The visible emission is still observed
even after 60 min. Our study has opened a new avenue for exploring green
and simple effective CL systems through AIE surfactant with unltralow
CMC toward various applications in lighting, optical sensing, and
photocatalysis, etc.
Prediction Model and Demonstration of Regional Agricultural Carbon Emissions Based on PLS-SA-AdaBoost: A Case Study of Fujian Province, China
Authors: Qi Yanwei , Liu Huailiang , Zhao Jianbo
Published: March 2023

The prediction of regional agricultural carbon emissions is of great
significance to regional environmental protection and sustainable
development of regional agriculture. This paper puts forward a combined
prediction model integrating Partial Least Squares (PLS), Simulated
Annealing (SA) and Adaptive Boosting (AdaBoost) to predict regional
agricultural carbon emissions, which overcomes the shortcomings of
insufficient accuracy of a single model prediction. This paper conducts
a demonstrative study on the agricultural carbon emissions in Fujian
Province, China to verify the feasibility and effectiveness of the
PLS-SA-AdaBoost combined prediction model. The experimental results show
that PLS-SA-AdaBoost combined prediction model has a higher precision
than SA-AdaBoost model and PLS-SA-AdaBoost model; meanwhile
PLS-SA-AdaBoost combined prediction model shows obvious advantages
compared with other combined prediction models. In terms of five
different scenarios, the paper adopts PLS-SA-AdaBoost combined
prediction model to predict the future trend of agricultural carbon
emissions in Fujian Province.
Evolutionary dynamics of Euphorbia carniolica suggest a complex Plio-Pleistocene history of understorey species of deciduous forest in southeastern Europe
Authors: Kirschner Philipp , Záveská Eliška , Huelber Karl , Wessely Johannes , Willner Wolfgang , Schönswetter Peter , Frajman Bozo
Published: March 2023

Deciduous forests form the dominant natural vegetation of Europe today,
but were restricted to small refugia during Pleistocene cold stages,
implying an evolutionary past shaped by recurrent range contractions and
expansions. Cold-stage forest refugia were probably widespread in
southern and central Europe, with the northwestern Balkan Peninsula
being of particular importance. However, the actual number and location
of deciduous forest refugia, as well as the connections between them,
remain disputed. Here, we address the evolutionary dynamics of the
deciduous forest understorey species Euphorbia carniolica as a proxy for
past forest dynamics. To do so, we obtained genomic and morphometric
data from populations representing the species’ entire range,
investigated phylogenetic position and intraspecific genetic variation,
tested explicit demographic scenarios and applied species distribution
models. Our data support two disjoint groups linked to separate refugia
on the northwestern and central Balkan Peninsula. We find that genetic
differentiation between groups started in the early Pleistocene via
vicariance, suggesting a larger distribution in the past. Both refugia
acted as sources for founder events to the southeastern Alps and the
Carpathians, whereas the latter were likely colonized before the last
cold stage. In line with traditional views on the pre-Pleistocene origin
of many southeastern European deciduous forest species, the origin of E.
carniolica was dated to the late Pliocene. The fact that E. carniolica
evolved at a time when a period of continuous forestation was ending in
much of Eurasia provides an interesting biogeographical perspective on
the past links between Eurasian deciduous forests and their biota.
HD-NET: Humerus deep-net for humerus fracture and bony callus formation analysis
Authors: Tariq Abdullah , Shoaib Muhammad , Arshad Dr.Shazia , Khurshid Dr.Syed Khaldoon , Iqbal Dr.Faiza , Javaid Abqa
Published: March 2023

When employing x-ray images, fracture identification in orthopaedics is
a difficult task. A large percentage of humerus fracture patients are
seen in hospitals, particularly in their emergency departments. Similar
to this, after a fracture, accurate callus production monitoring is
crucial for bone healing. Thus, a fractured patient’s diagnosis and
therapy must be accurate and administered promptly. This work
investigates the use of deep learning on X-ray images of the humerus for
fracture snd bone callus formation analysis to help physicians in the
diagnosis of such fractures, especially in emergency settings.This study
is named HD-NET, which stands for Humerus Deep Net. The framework
includes image enhancement using a Gaussian filter and histogram
equalization, two-stage object detection, image super-resolution, U-NET
segmentation with feature recalibration. Finally, an LSTM with a
sequence length of 2 is used to analyze callus formation at the fracture
site. The LSTM takes the segmented area as input and outputs a
prediction for the stage of healing and potential complications. The
proposed framework was evaluated on a combination of the MURA dataset
and a self-collected dataset.Results demonstrated that in terms of
specificity, sensitivity, and accuracy, the suggested framework
performed better than earlier studies. This research can be expanded to
different bone types and is useful for orthopaedic practitioners.
Human Resource Management Practices and their potential relationship with Organizational Citizenship Behavior in educational institutes.
Authors: Khan Mubarak  Zaib , Niqab Muhammad , Hanson Janet
Published: March 2023

The current trends in technology and globalization has encouraged Human resource management practices to adopt optimized strategies in dealing with challenges face by the educational institutions. In order to achieve the desired results and outcomes in educational institutions in a definite timeframe, valuable policies, capabilities and values need to be implemented. For creating a sustainable organizational environment, the organizational citizenship behavior (OCB) has been developed for supportive and cooperative interaction and relationship between the employer and employee by cooperating with each other for effective running of the schools. The current study is aimed to investigate the relationship between HRM practices and OCB in higher secondary schools in Dir Lower& Upper. This study is quantitative in nature and data was collected through closed ended questionnaire. The collected data was analyzed with person correlation techniques. It is  found that HRM practices has a notable relationship with organizational citizenship behavior in higher secondary schools in Pakistan and results further speak out that performance appraisal has a high relationship with OCB while the rest of practices have low relationship with OCB.  After discussion in light of available literature, recommendations has been framed for educational institutions and policy maker.
A moderated-mediation analysis of supply chain efficiency, flexibility, integration, and risk management
Authors: Bhatti Asif Ali , Bhatti Sabeen  Hussain , Saif Salman
Published: March 2023

The preparedness and stability of the defense-industry supply chains have been challenged by significant disruptions and risks that can be critical to national security. Despite increasing importance, limited studies have explored the underlying factors (i.e., efficiency, flexibility, integration, and risk management) impacting the supply chain operations of national defense companies. Hence, this study aims at exploring the relational impact of supply chain risk management (SCRM) practices and organizational performance by hypothesizing a moderated-mediation model involving operational efficiency, flexibility, and supply chain integration (SCI). Using the information processing theory, the survey-based data of 2017 respondents was collected from defense-related national institutions in Pakistan. Empirical findings based on structural equation modeling validated a significant positive SCRM è Organizational Performance relationship. According to the findings, supply chain risk management is strongly connected to operational efficiency and flexibility. Additionally, the study also substantiates the mediating effects of operational performance (i.e., efficiency and flexibility), along with the significant moderation by supply chain integration in the relationship between risk management and operational efficiency. However, the results do not support the role of operational flexibility in mediating the relationship between SCRM and OP. Furthermore, the role of SCI as a moderator between SCRM è operational flexibility was not established. This research is the first to introduce an empirically validated framework of supply chain performance in the defense industry, besides conceptualizing the direct and indirect roles of SCRM, efficiency, flexibility, and integration. This study also navigates some of the critical issues and obstacles faced by the national defense industry and highlights a holistic model that facilitates a multistage supply chain decision-making.
Evaluation of derivatives of 2,3-dihydroquinazolin-4(1H)-one as inhibitors of cholinesterases and their antioxidant activity: In vitro, in silico, and kinetics studies
Authors: Babatunde Oluwatoyin , Hameed Shehryar , Mbachu Kingsley , Saleem Faiza , Chigurupati Sridevi , Wadood Abdul , Ur Rehman , Venugopal Vijayan , Khan Khalid , Taha Muhammad , Ekundayo Olusegun , Khan Maria
Published: March 2023

In search of potent inhibitors of cholinesterase enzymes and antioxidant agents, synthetic derivatives of dihydroquinazolin-4(1H)-one (1?38) were evaluated as potential anti-Alzheimer agents through in vitro acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitions and radical (DPPH and ABTS) scavenging activities. The structure?activity relationship (SAR) was mainly based on the different substituents at the aryl part which showed a significant effect on the inhibitory potential of enzymes and radical scavenging activities. The kinetic studies of most active compounds showed a noncompetitive mode of inhibition for AChE and a competitive mode of inhibition for the BChE enzyme. Additionally, molecular modelling studies were carried out to investigate the possible binding interactions of quinazolinone derivatives with the active site of both enzymes.
Numerical investigation on cooling rate in proton exchange membrane fuel cell using propylene glycol fluid
Authors: Sahoo Dillip , Ram Sri , Prasath Sriram
Published: March 2023

In this study, a cooling channel was constructed inside the fuel cell to examine the impact of cooling on proton exchange membrane fuel cell performance. The performance of the fuel cell was assessed using four different coolant mixtures: DI100 (100% deionized water ? DI), PG10 (90% DI water + 10% propylene glycol), PG20 (80% DI water + 20% propylene glycol), and PG30 (70% DI water + 30% propylene glycol). The efficiency of the fuel cell, system temperature, operating parameters, coolant, and cooling channel shape of the fuel cell were tested using a CFD model based on the finite volume approach. The test results showed that the fuel cell performance was good for both single-cell fuel cells and fuel cell stacks at temperatures of 354 K and 360 K, respectively. However, as the membrane became dehydrated above 362 K for single cell fuel cells and after 371 K for fuel cell stacks, performance of the fuel cell decreased and no appreciable improvement was seen. For single cells, the fuel cell showed good performance improvement at PG30 combinations, whereas the best performance in stacks was attained at PG20 combinations.
Numerical simulation of plasma gasification of oil-based drilling cuttings
Authors: Hu Liang , Yu Hailong , Xu Yayun , Duan Xinying , Wu Xulei , Sun Yunlan , Chen Haiqun , Zheng He , Wu Bo
Published: March 2023

In this paper, ASPEN PLUS commercial software is used to simulate the plasma gasification process of oil-based drilling cuttings, and the numerical simulation results are analyzed in detail. The conclusions can provide theoretical guidance for subsequent experiments and practical engineering applications. A set of reactor models suitable for gasification of such substances was established by using Gibbs? free energy minimization principle, and the accuracy of the model was verified by comparing the numerical simulation results with the experimental results. In this model, the effects of plasma torch power, air equivalent ratio, moisture content of feed, and the amount of CO2 added on the gasification results were simulated. Through systematic analysis, the optimal gasification parameters are determined output power of plasma torch is 27.5 kW, air equivalent ratio is 0.26, moisture content of feed is 6%, and the amount of CO2 added is 6 kg per hour.
Optimization of physical property and working condition parameters in multi-size sinter vertical tank based on exergy analysis
Authors: Zhang Si-Zong , Huang Lin-Jing , Wen Zhi , Liu Xun-Liang
Published: March 2023

Aiming at the characteristics of multi-size particles in the waste heat recovery sinter vertical tank, a steady-state gas-solid heat transfer model of multi-size ver?tical tank was established. Based on exergy analysis, the influences of physical properties and working conditions on the gas-solid heat transfer and the waste heat recovery capacity of the vertical tank were studied. Finally, the optimum pa?rameter combination was obtained by the orthogonal test method. Results showed that the mono-size sinter was no longer suitable for analyzing the gas-solid heat transfer process of the vertical tank in the actual production. The total exergy of the multi-size sinter vertical tank increased first and then decreased with the in?crease of aspect ratio in the range of 0.39-1.75, and reaches the peak value when height-diameter ratio is 0.7. The total exergy increased with the increase of tem?perature difference between gas-solid inlet temperature. The total exergy increased first and then decreased with the increase of gas-material ratio in the range of 610-1170 m3 per tone, and reached the maximum value at the gas-material ratio of 850 m3 per tone. The exergy decreased with the increase of porosity in the range of 0.548-0.603. The most suitable vertical tank parameters were height-diameter ra?tio 0.44, sinter inlet temperature 800?C, gas inlet temperature 25?C, gas-material ratio 770 m3 per tone and porosity 0.601.
Study on the simplified model of vertical double U-pipe ground heat exchanger
Authors: Wang Xin , Fu Qiang , Wang Changlong
Published: March 2023

A simplified semi-analytical model of vertical double U-pipe ground heat exchanger was established. The validity of the established model is examined by contrasting the figured outcomes with experiment data, emulation results of 3-D numerical model and calculation results of infinite line-source model for different inlet boundary conditions and configurations. After one hour, the semi-analytical model?s relative error is less than 0.32% under the boundary condition of given inlet fluid temperature. Under the boundary condition of given total heat input rate, the semi-analytical model?s relative error after ten hours is less than 0.11%, while the infinite line-source model?s relative error is less than 0.60%. The semi-analytical model is in good agreement with experiment and numerical model, and has higher calculation accuracy than infinite line-source model.
Study on the effect of ash layer on flow and heat transfer in diesel particulate filter based on 3-D thermal lattice Boltzmann method
Authors: Li Xiangdong , Zhang Tiechen , Liu Xiaori
Published: March 2023

After the Diesel engine works for a long time, dense ash layer will be formed on the diesel particulate filter surface, which will increase the back pressure on the diesel particulate filter surface and seriously affect the normal operation of the diesel particulate filter. The diesel particulate filter micro-structure is generated by 3-D reconstruction technology. Moreover, the particle layer and ash layer are reconstructed by quartet structure generation set. The exhaust gas with a velocity of 0.05-0.25 m/s is introduced into the diesel particulate filter model. Under the condition of 873 K temperature field, the 3-D lattice Boltzmann method is used to explore the influence of the particle layer and ash layer accumulated in its micro-structure on its flow and heat transfer. The results show that with the accumulation of ash, the flow velocity in the diesel particulate filter increases with the decrease of porosity. The existence of ash layer increases the pressure difference between the inlet and outlet of the structure, and the pressure difference between the inlet and outlet of the structure increases with the increase of the air inlet speed. Under the same flow conditions, the heat transfer process of structures containing ash stratification is faster, and the highest temperature point moves forward accordingly. This has certain guiding significance for exploring the thermal load of ash containing diesel particulate filter structure.
Research on geometric inverse problem based on the firefly conjugate gradient method
Authors: Wang Shoubin , Li Yunlong , Peng Guili , Xu Wenbin , Zhou Xuejun , Ju Yongqiang
Published: March 2023

In this paper the 2-D steady-state heat transfer geometric inverse problem is solved using the finite element method, conjugate gradient method, and firefly algorithm. Based on the finite element method for solving the forward heat transfer model, and based on continuous iterative optimisation of the conjugate gradient method, the accuracy of the error function of the measured and estimated values is kept within a certain range, so that the geometry of the object under test can be calculated in an inverse way. In the study of the forward problem, the temperature field distribution is solved using circular pipes as the research objects, and the feasibility of applying the finite element method to heat transfer problems is verified. The inverse problem takes the circular tube as the object and considers two different corrosion defects on the inner wall of the circular tube. Simultaneously, the firefly algorithm is introduced based on the conjugate gradient method to stochastically optimize the temperature data and suppress the fluctuation of the inversion result. Numerical experiment results indicate that the method in this paper can perform more accurate geometric shape recognition when there is a certain temperature measurement error or the temperature measurement information is relatively complete.
Lymphocyte-epidermis interactions in malignant epidermotropic lymphomas: I. Ultrastructural aspects
Authors: Schmitt D. , Thivolet J.
Published: March 2023

The interactions between lymphocytes, Langerhals cells and keratinocytes, are described by electron microscopy in cutaneous lesions of malignant epidermotropic lymphomas (mycosis fungoides and Sézary's syndrome). Various contacts are observed between the different cells. During the degenerating process of keratinocytes, Langerhans cells disappear and histiocytic cells are bound to necrosing epidermal cells. The immunological process of Pautrier microabscess formation is discussed by comparison with ultrastructural aspects of cutaneous lecions of G.V.H. reaction in man.
Immunohistochemical study on 70 human embryos concerning the epidermal binding of pemphigus and pemphigoid antibodies
Authors: Varelzidis A. , Tosca A. , Coumantakis E. , Nicolis G. , Hatzis J. , Papavasiliou S. , Stratigos J.
Published: March 2023

In the present study, the development of pemphigus and bullous pemphigoid antigens was investigated by means of the indirect immunofluorescence technique using sera of pemphigus and bullous pemphigoid patients, respectively, on human foetus skin antigenic substrate. Seventy skin specimens from embryos of 9-38 weeks of gestation were studied. Both pemphigus and bullous pemphigoid antigens were observed for the first time at about 16 weeks of gestation. Pemphigus antigen has a slower rate of evolution. Between 30 and 38 weeks both antigens were detected as strongly positive.
Association between psoriasis and the alpha 1-antitrypsin deficiency gene Z
Authors: Beckman G. , Beckman L. , Lidén S.
Published: March 2023

In a series of 72 psoriatic patients the frequency of individuals carrying the alpha 1-AT deficiency gene Z was found to be significantly (p less than 0.001) increased.
HLA antigens in methotrexate-induced liver cirrhosis
Authors: Zachariae H. , Kragballe K. , Thestrup-Pedersen K. , Kissmeyer-Nielsen F.
Published: March 2023

HLA typing was carried out in 20 psoriatics with methotrexate-induced liver cirrhosis and 44 methotrexate-treated psoriatics who at the time of investigation had not developed cirrhosis. The data were compared with results from tissue typing of 1291 healthy individuals. The antigen combination A1, B8 was found to be increased among patients with cirrhosis vis-à-vis methotrexate-treated psoriatics who did not develop liver cirrhosis. This finding is similar to data on chronic active hepatitis type A and drug-associated chronic active hepatitis. Findings of the antigen combination A1, B8 should--just as with other predisposing factors--lead to caution in liver control in psoriatics on methotrexate therapy.
REM syndrome: an immediate therapeutic response to hydroxychloroquine sulphate
Authors: Balogh E. , Nagy-Vezekényi K. , Fórizs E.
Published: March 2023

A 22-year-old woman had been suffering for 11 years with a REM syndrome. Her symptoms were provoked and aggravated by exposure to sunlight. The administration of hydroxychloroquine sulphate had a quick effect. The drug was given for 6 months, and the patient remained symptom-free even in summer, when sunbathing.
Topical application of zinc-solutions: a new treatment for herpes simplex infections of the skin?
Authors: Wahba A.
Published: March 2023

Eighteen patients experiencing 22 episodes of recurrent herpes simplex skin infections were treated with topical applications of a solution containing 4% zinc sulphate in water. In all patients, pain, tingling and burning stopped completely within the first 24 hours of zinc therapy. Crusting occurred within 1-3 days and no adverse effects were observed.
Plasma concentrations after bath treatment and oral administration of trioxsalen
Authors: Fischer T. , Hartvig P. , Bondesson U.
Published: March 2023

An analytical method comprising homogenous extraction and determination by gas chromatography mass spectrometry has been developed for the quantitation of trioxsalen in plasma. The limit of sensitivity of the method has been 2 ng/ml of trioxsalen. The method was used to monitor concentrations of trioxsalen in plasma after a 40 mg oral dose or after trioxsalen bath treatment. The concentrations in plasma after oral administration did not exceed 3 ng/ml, while in 2 patients after bath treatment trioxsalen could be determined (2-3 ng/ml). The low plasma concentrations found throw light on the poor UV-sensitizing properties found after oral trioxsalen therapy.
Pachymeningitis cervicalis hypertrophica syphilitica
Authors: Agdal N. , Hagdrup HK. , Wantzin GL.
Published: March 2023

A rare form of neurosyphilis with hypertrophic cervical pachymeningitis is presented. It is concluded that serological tests for syphilis are still indicated in patients with unexplained neurological or psychiatric symptoms.
Binding specificity of guinea pig anti-alpha-keratin polypeptide sera on human keratinocytes: comparison of their receptors with those of human epidermal cytoplasmic antibodies
Authors: Viac J. , Schmitt D. , Staquet MJ. , Thivolet J. , Ortonne JP. , Bustamante R.
Published: March 2023

Experimental anti-keratin polypeptide sera (KPS) which were prepared by immunizing guinea pigs with the P1 polypeptide (molecular weight: 67 0000 dalton) of alpha-keratin of normal human stratum corneum, were shown to react in immunofluorescence only to cell cytoplasmic antigen of the upper layers of the epidermis. No staining was detected in the basal layer. An immunolabeling performed on free epidermal cells obtained after trypsinization demonstrated by electron microscopy that receptors for KPS were tonofilaments. Minor proportions of negative cells containing tonofilaments and numerous melanosomes detected might correspond to the basal cells. An IF pattern similar to that seen with KPS was observed with some human epidermal cytoplasmic sera (ECS). However, reciprocal blocking tests performed on both rabbit lip and normal human skin with the different sera showed no inhibition. No cross-reaction was detected by immunodiffusion test between whole purified alpha-keratin and human ECS. In electron microscopy, the receptors for ECS appeared not to be keratin-like, but were located on a granular part of the peripheral keratinocyte cytoplasm. These findings confirmed two steps (basal and malphighian layers) in epidermal differentiation defined by antigenic markers and especially by the alpha-keratin component of MW 67 000 d present in normal stratum corneum.
Ultrastructural changes of zinc deficient rat epidermis: an electron microscopic study
Authors: Weismann K. , Christophersen J. , Kobayasi T.
Published: March 2023

Zinc deficiency was produced in rats by feeding them a diet containing 0.5 mg zinc per kg. After 2 weeks the zinc-deficient rats showed markedly stunted growth as compared with control rats receiving an identical diet supplemented with 50 mg zinc per kg. After about 3 weeks all rats in the zinc-deficient group developed a greasy, coarse and scaly coat with dispersed, denuded, eczematous patches. After 32 days the animals were sacrificed and skin specimens were obtained from the abdominal area. Electron microscopy showed two different patterns of epidermal changes: (A) edematous cell bodies and cell organelles, degenerate nuclei, while desmosomes were well preserved: (B) parakeratotic and dyskeratotic cells with sparse keratohyalin granules, abnormal keratinosomes, poorly developed desmosomes, and disintegrated mitochondria in the upper part of the epidermis. In the lower part, keratinocytes contained numerous ribosomes and thick chromatin aggregates in the nuclei. Dendritic cells were disintegrated in pattern A, while they appeared normal in pattern B. In both patterns mitochondria showed vacuoles and a lack of DNA granules. A transient form of type A and B changes was also observed.
Effects of PUVA on the epidermal melanocyte population in psoriatic patients
Authors: Rosdahl IK. , Swanbeck G.
Published: March 2023

The effect of PUVA treatment on the epidermal melanocyte system was studied in 24 skin biopsies from four male psoriatic patients. The patients had received 40 mg 8-methoxypsoralen and a phototoxic dose of UVA (2-12 joule/cm2), twice a week. Punch biopsies were taken from unaffected and psoriatic skin in the gluteal region before, and after three and nine treatments. The epidermal melanocytes were counted in dopa incubated split-skin preparations and the mitotic index of the keratinocytes was monitored as a control of the PUVA effect. The epidermal melanocyte number was essentially normal in non-treated psoriatic patients. During the PUVA treatment the melanin production and the DOPA activity of the melanocytes increased markedly. However, there was no significant change in the number of DOPA active melanocytes, either in the unaffected or the psoriatic skin. Thus, PUVA treatment is not necessarily associated with an increase in the number of active epidermal melanocytes.
Effect of serum and oxygen tension on human skin organ culture: a histometric analysis
Authors: Tammi R. , Jansén C.
Published: March 2023

No abstract available
Carotenoid treatment for light sensitivity: a reappraisal and six years' experience
Authors: Wennersten G.
Published: March 2023

Long-term treatment with oral carotenoids in 57 patients suffering from a variety of photodermatoses and disorders associated with cutaneous light sensitivity was evaluated. All patients were treated for two or more 6-month periods in separate years. Best therapeutic results were seen in PMLE patients, a good to excellent therapeutic response was noted for 65% of all patients, increasing to 81% of those assigned to skin types III and IV, and decreasing to 47% of those with skin types I and II. The therapeutic effect observed in disorders characterized by other mechanisms than provocation by solar radiation per se was less conspicuous, viz. for light-sensitive psoriasis and lupus erythematosus. Even here, therapeutic failure seems to be more common in individuals with skin types I and II than for skin types III and IV. Photodermatoses such as persistent light reaction, actinic reticuloid and solar urticaria did not respond to any significant degree to carotenoid treatment. Our findings would appear to justify further treatment with oral carotenoids in selected cases of PMLE, and a higher dosage level may be tried for non-responding individuals with light-sensitive psoriasis and DLE or SLE. Serious side effects have not been observed in spite of long-term therapy lasting several years.
Environmental modification of the shape of pigment-producing cells
Authors: Paul E.
Published: March 2023

With fluorescence-histochemical methods (FIF method) pigment-producing cells were investigated in benign and malignant skin lesions. Here, pigment cells appear predominantly dendritic, whilst they are separated from each other by keratinocytes, cells of hair follicles, or cells of sebaceous glands. In lentigo maligna melanoma, superficial spreading melanoma as well as in nodular melanoma and some metastases of these melanomas, the tumour cells can show a dendritic pattern under special environmental situations. It is, therefore, assumed that the shape of tumour cells in melanomas is more closely dependent on their particular environment and other modifying factors than on a different histogenesis.
Cefuroxime treatment of urethritis caused by a beta-lactamase-producing strain of Neisseria gonorrhoeae
Authors: Normark S. , Elmros T. , Norlander L. , Kjellberg E. , Holm S. , Burman LA. , Bergström S.
Published: March 2023

A patient who contracted urethritis from a beta-lactamase-producing strain of Neisseria gonorrhoeae was successfully treated with the cephalosporin derivative cefuroxime. As expected, neither cefuroxime nor cefamandole was hydrolysed by plasmid-coded gonococcal beta-lactamase. Cefuroxime ought to be a valuable and efficacious substitute for penicillins in the treatment of gonhorrhoea due to beta-lactamase-producing gonococcal strains.
Ia-like antigens in lichen planus
Authors: Tjernlund UM.
Published: March 2023

The occurrence of Ia-like antigens in the skin of patients with lichen planus was studied by an indirect immunofluorescence (IIF) technique with use of immunosorbent-purified anti-Ia antibodies. Normal skin from the patients and skin from healthy persons showed dermal histiocytes and epidermal suprabasal dendritic cells expressing these antigens. In lichen planus lesions Ia-like antigens were found on virtually all mononuclear cells in the dermal infiltrate and showed an intercellular pattern in the epidermis. The finding of cells expressing Ia-like antigens in the dermal infiltrate, most probably activated T lymphocytes, renders likely a cell-mediated type of reaction in the pathogenesis of lichen planus.
Immunofluorescent studies of complement C3 in the hair follicles of normal scalp and of scalp affected by alopecia areata
Authors: Igarashi R. , Takeuchi S. , Sato Y.
Published: March 2023

The deposition of complement C3 in the hair follicles of scalps of 4 normal subjects and of the affected parts of 9 patients with alopecia areata was found by the direct immunofluorescent methods. No deposition of immunoglobulins (IgG, IgA, IgM, IgD and IgE) was found. In the normal scalps, complement C3 was found to be deposited in the hyaline membrane of the hair bulb and in the internal layer of connective tissue sheath at the lower about 2/3 transient portion of anagen follicles, and in the thickened hyaline membrane of the catagen follicles. The deposition of complement C3 was also found in the anagen and catagen-like hair follicles even in the patients with alopecia areata, which was similar to the finding in the normal scalps. From the fact that the deposition of complement C3 was found chiefly in the transient portion of the hair follicles of the normal scalps, and that the behavior of deposition of complement C3 reflected the morphological state of hair follicles in each stage of the hair cycle, even in alopecia areata, complement C3 may be intimately related to the hair cycle, even though its significance is as yet unknown.
Immunofluorescence study in purpura pigmentosa chronica
Authors: Iwatsuki K. , Aoshima T. , Tagami H. , Ohi M. , Yamada M.
Published: March 2023

Purpura pigmentosa chronica comprises a group of vascular disorders of unknown etiology. In biopsied specimens obtained from 8 patients, we observed vascular changes such as fibrinoid degeneration and occlusive damage occasioned by a hyalinized substance as well as swollen endothelia in 3. However, in all biopsied specimens, direct immunofluorescence studies demonstrated depositions of C3 or C1q with or without immunoglobulins, and of fibrin in papillary vessels. These findings suggest the possibility that immunological processes are involved in producing the histopathologic features of this disorder.
Antireticulin antibodies in psoriasis
Authors: Siri A. , Lozano W. , Vignale RA.
Published: March 2023

Antireticulin antibodies were studied in the sera of 32 patients affected by various clinical forms of psoriasis. The indirect immunofluorescence method was used on histological sections of human kidney and mouse liver and kidney. This method was also used with sera from children with coeliac disease and from healthy controls. In 10 these antibodies were positive, while in 22 they were negative, the intensity of the titres varying with the extent of the disease. These results support the notion that immunological phenomena may be involved in the physiopathological mechanisms of psoriasis.
Prevalence of skin diseases in old age
Authors: Weismann K. , Krakauer R. , Wanscher B.
Published: March 2023

The prevalence of skin disorders was studied in an elderly population comprising 584 individuals. The mean age was 80 years (range 55-106). The prevalence of pityriasis of the scalp and asteatosis was high, 44.3% and 28.9%, respectively. Psoriasis vulgaris was found in 2.9%, vitiligo in 1.2%, and contact dermatitis in 3.8% of the group.
Circulating anticoagulant and serological tests for syphilis
Authors: Shoenfeld Y. , Shaulian E. , Shaklai M. , Kruglack J. , Feuerman E. , Pinkhas J.
Published: March 2023

Circulating anticoagulant (CA) and particularly lupus anticoagulant are commonly associated with biological false-positive tests for syphilis (BFP-STS) in patients with collagen diseases. CA was presently found in the sera of 8 out of 30 subjects with chronic BFP-STS without collagen or autoimmune diseases. It was not found in any of the 21 patients with various stages of syphilis. In 5 out of 21 elderly subjects (age greater than 70 years) in whom a positive BFP-STS was detected, there was no CA. It is concluded that the association between CA and BFP-STS is found only in patients with collagen and autoimmune diseases and in some of the younger chronic BFP-STS reactors. It is not detected in syphilitic patients or elderly subjects in whom a high incidence of BFP-STS can be found. The difference in the incidence of this association is probably due to the differing biologic behaviour of these autoantibodies.
Demonstration of circulating and tissue-fixed immune complexes in cutaneous necrotizing vasculitis
Authors: Imamura S. , Yanase K.
Published: March 2023

Simultaneous demonstration of circulating and tissue-fixed immune complexes was attempted in 22 patients with cutaneous necrotizing vasculitis (7 anaphylactoid purpura, 9 cutaneous allergic vasculitis, 2 livedo reticularis, 1 thrombophlebitis, 2 erythema elevatum diutinum and 1 acute generalized pustular bacterid). In 16 out of the 22 patients, particularly patients with anaphylactoid purpura and cutaneous necrotizing vasculitis, there was a high Clq-binding activity. Decreased levels of C3 and C4 were seen in 2 and 3 patients, respectively. In 11 out of 16 skin lesions, the granular deposits of immunoglobulins and/or complement were demonstrated in the blood vessel walls of the dermis. IgA deposit was seen in anaphylactoid purpura, and IgM deposit in other types of vasculitis. C3 deposit was the most frequently noted. There was no definite correlation between Clq-binding activity and tissue deposits.
5-S-cysteinyldopa as a substrate for tyrosinase
Authors: Hansson C. , Rorsman H. , Rosengren E.
Published: March 2023

Oxidation of 5-S-cysteinyldopa by mushroom tyrosinase was studied by measuring 5-S-cysteinyldopa consumption with HPLC. 5-S-cysteinyldopa was found to be a substrate for tyrosinase, but our results suggests that a self-catalysed oxidation induced by enzymatically formed 5-S-cysteinyl dopaquinone also takes place. Dopa oxidation by tyrosinase was determined by measuring substrate consumption with HPLC. The oxidation of 5-S-cysteinyldopa was markedly accelerated in the presence of dopa. This could be explained by dopaquinone oxidation of 5-S-cysteinyldopa, which has a lower oxidation potential than dopa.
Allergy to human seminal plasma: a presentation of six cases
Authors: Kroon S.
Published: March 2023

Six women had symptoms of immediate type allergic reactions such as localized oedema, urticaria and pruritus elicited at, or soon after intercourse. All had positive skin prick tests with dilutions of human seminal plasma. The symptoms failed to appear when the women's partners used a condom an intercourse. None of the women had IgE antibodies in serum against human seminal plasma when tested with the radioallergosorbent test. This type of allergic reaction is easily overlooked.
Vaccinia and topical steroids: a case report
Authors: Gundersen SG. , Bjorvatn B.
Published: March 2023

Widespread vaccinia developed in non-eczematous skin around the eyes and mouth of a 20-year-old man, whereas active eczematous lesions on both wrists were unaffected. The use of steroid ointments is discussed as a possible causal factor.
The pachyonychia congenita syndrome
Authors: Schönfeld PH.
Published: March 2023

This study presents two patients showing different clinical types of the Pachyonychia congenita Syndrome (PcS). For the first time there is a description of the histopathology of the blister and vesicle formation, as well as of the plantar keratosis. The essential resemblance in the histopathological changes of the different symptoms of the syndrome is discussed.
Electron microscopic observation of infiltrating neutrophils in aphthous ulceration in Behçet's disease
Authors: Honma T.
Published: March 2023

Neutrophils infiltrating the site of aphthous ulceration of the oral mucosa of patients with Behçet's disease were studied in the electron microscope. Infiltrating neutrophils without degenerating in the lamina propria are frequently phagocytosed by large mononuclear cells.
Superficial actinic porokeratosis in a patient undergoing long-term PUVA therapy
Authors: Reymond JL. , Beani JC. , Amblard P.
Published: March 2023

No abstract available
Glaucoma and amaurosis associated with long-term application of topical corticosteroids to the eyelids
Authors: Vie R.
Published: March 2023

The development of glaucoma and amaurosis in a 29-year-old woman with atopic dermatitis is described. The association of the eye involvement with many years of daily application of fluorinated corticosteroid ointment to the eyelids is suggested.
Absence of eccrine sweat glands without other ectodermal defects
Authors: Wantzin GL. , Kobayasi T.
Published: March 2023

No abstract available
Topical treatment of penile condylomata acuminata with colchicine at 48-72 hours intervals
Authors: Von Krogh G. , Rudén AK.
Published: March 2023

We previously demonstrated that 8% solutions of colchicine in ethanol eradicated penile warts in 36% of patients after a single application and in an additional 12% after a second application performed at least a week after the first. In the present study only 30% of 115 men were cured when penile warts were painted twice at intervals of 48 hours with 2% solutions and 72 hours with 4% and 8% solutions of colchicine. The lack of additive therapeutic effect accruing from the second application and the high frequency of intolerable side effects when using the latter treatment program indicate that this regimen is unsuitable for condylomata therapy.
Differences in response of psoriatic epidermis in cyclic AMP accumulation against certain adenyl cyclase agonists
Authors: Yoshikawa K. , Mori N. , Hadame K. , Sakakibara S.
Published: March 2023

Epidermal slices of pig skin and psoriatic human skin were used in a study on responses of adenyl cyclase to epinephrine, histamine and adenosine. In pig skin, histamine stimulated adenyl cyclase slightly more than epinephrine. The histamine concentration eliciting the maximum cyclic AMP accumulation was 5 x 10(-4)M, which was 20 times greater than that of epinephrine. The optimal concentration of adenosine seemed to be an additional 10 times higher (5 x 10(-3)M). When involved and uninvolved epidermal specimens from 18 psoriatic patients were incubated with these three agents, cAMP formation with histamine was unchanged or slightly increased in involved epidermis, while the response to epinephrine was markedly depressed and to adenosine moderately so. These findings suggest further that there is a selective defect in the membrane enzyme system in psoriatic epidermis.