bluuebunny/crossref_metadata_2025 huggingface dataset:
Tratamento cirúrgico para o ceratocisto odontogênico e análise das suas características imaginológicas e histopatológicas: Relato de caso
Authors: Figueiredo Neto Félix Augusto , Silva Ana Júlia Carla de Oliveira , Souto Elmano Marques Martins da Costa , Pereira Mateus José Carvalho , Soares Betânia Maria , Grandinetti Helenice de Andrade Marigo , Castro Carlos Henrique Bettoni Cruz de , Resende Renata Gonçalves
Published: January 2024

O Ceratocisto Odontogênico (CO) é uma lesão que acomete os ossos da mandíbula e maxila, de crescimento lento e assintomático. Se origina do tecido odontogênico por proliferação do tecido mesenquimal, epitelial, ou de ambos, com produção de ceratina em seu interior. O tratamento do CO acontece em diferentes etapas e abordagens terapêuticas, portanto, o objetivo desse artigo é descrever, através de um relato de caso, o tratamento realizado para um CO desde a conclusão da marsupialização, abordagem cirúrgica realizada e acompanhamento pós-operatório de dois anos em um paciente masculino de 15 anos. A lesão apresentava-se radiolúcida, unilocular, com halo radiopaco, crescimento anteroposterior sem destruição da cortical óssea e a abordagem clínica inicial consistiu na realização de punção aspirativa e biopsia incisional cuja análise histopatológica confirmou o diagnóstico de CO. Sendo assim, realizou-se a marsupialização com cânula a fim de estimular o processo de neoformação óssea e reduzir o tamanho da lesão para ser favorável removê-la completamente, além de conferir uma margem segurança às estruturas nobres adjacentes. Com seis meses de descompressão da lesão, o paciente foi submetido a enucleação cirúrgica da lesão associada a osteotomia periférica. O aspecto radiográfico, dois anos após a cirurgia, mostra neoformação óssea local, integridade de estruturas nobres como o canal mandibular e sem evidência de recidiva da lesão. O paciente será acompanhado por mais três anos, a cada seis meses.
Pemanfaatan media sosial sebagai media promosi, branding pada produk UMKM olahan pisang dan olahan susu
Authors: Sari Ardilla Khusliana , Sulhan M.
Published: January 2024

Berdasarkan Data Kementerian Koperasi dan Usaha Kecil dan Menengah (Kementerian KUKM) pada tahun 2021, jumlah pelaku UMKM di Indonesia mencapai 64,2 juta dengan kontribusi terhadap produk domestik bruto (PDB) sebesar 61,07 % atau Rp8.573,89 triliun. UMKM mampu menyerap 97 % dari total angkatan kerja dan mampu menghimpun hingga 60,4 % dari total investasi di Indonesia. (kementrian Keuangan RI) UMKM memiliki peran penting bagi perekonomian Indonesia terutama saat terjadinya krisis, UMKM dapat meliwati Krisis moneter pada saat masa pandemi COVID-19, namun banyak pelaku UMKM yang mengaku usahanya mengalami penurunan penjualan di karenakan adaptasi penjualan akibat COVID-19 yang tadinya bisa dilaksanakan secara offline harus menyesuaikan menjadi pemasaran secara online, seperti halnya dua UMKM yang ada di Kecamatan Tutur Kabupaten Pasuruan ini. Letak Kecamatan Tutur sendiri berjakarak sekitar 24 km dari Universitas Yudharta Pasuruan. Kedua UMKM ini  memiliki produk yang terbuat dari sumber daya alam yang di hasilkan di Kecamatan Tutur seperti susu dan pisang mengingat Kecamatan Tutur adalah distributor susu sapi terbesar di Kabupaten Pasuruan. Namun semenjak terjadinya COVID-19 produk yang di produksi mengalami penurunan omset pembelian. Dengan adanya program pengabdian masyarakat yang di tugaskan kepada mahasiswa semester 7 Universitas Yudharta penulis melakukan pembimbingan dan pelatihan promosi produk dengan cara sederhana melalui media sosial yang sering di gunakan oleh khalayak ramai.
Pengembangan ngaji hadis melalui software mausuah al-hadith al-sharif untuk mencegah pemahaman radikal
Authors: Rosyid Achmad Fathor , Ahsani Nasirudin Al
Published: January 2024

Pemahaman radikal merupakan permasalahan utama yang perlu dicegah di Kabupaten Jember. Bahkan ketua GP Ansor mengatakan Jember sudah masuk zona merah radikalisme.  Tentunya hal tersebut tidak bisa dibiarkan begitu saja. Perlu adanya upaya pencegahan pemahaman radikal yang dilakukan oleh pemerintah, tokoh masyarakat, serta akademisi. Pengabdian ini menggunakan metode Asset Based Community Development (ABCD). Pengabdian ini dilakukan dengan: a) Discovery, melalui observasi aset individu, sosial, dan agama, serta FGD; b) Dream, menyatukan mimpi Pimpinan Pondok, Kepala KUA, MWCNU, dan santri, c) Design, mengakomodir masukan pelatihan Software al-Mausuah al-Hadith al-Sharif, d) Define, mengkoordinasikan para tokoh, dan e) Destiny, melakukan pelatihan Software hadis. Pengembangan ngaji hadis diukur dengan teori Konstruktivisme, yaitu: a) menggunakan software hadis sebagai alat bantu, b) software hadis sebagai alat interaksi, c) membebaskan santri mengeksplorasi informasi, d) memfasilitasi santri untuk berdiskusi, e) menelurkan duta moderasi beragama. Pencegahan paham radikal dibuktikan dengan: a) Pimpinan Pondok dan santrinya menegaskan tidak perlu mengubah hukum di Indonesia, b) para santri lebih terampil dalam mengakses hadis, c) menegaskan prinsip kesetaraan, d) para santri berpikir sebelum membuat penilaian, e) menunjukkan sikap inklusivitas, f) Pimpinan pondok menitikberatkan pada penjelasan dan uswah, dan g) menegaskan kepercayaan pada Pancasila dan UUD 1945 sebagai dasar negara Indonesia.
Implementasi proses sertifikasi halal self declare dalam mendukung pertumbuhan UMKM
Authors: Ariska Nenda , Setiawan Romi Adetio , Setiawan Adi , Zaki Khozin
Published: January 2024

Dalam konteks pertumbuhan ekonomi negara, Usaha Mikro, Kecil, dan Menengah (UMKM) memegang peran sentral. Undang-Undang Nomor 33 Tahun 2014 tentang Jaminan Produk Halal (UU JPH) telah mengubah status sertifikasi halal dari yang semula sukarela menjadi wajib. Penerapan Undang-Undang ini menekankan pentingnya peran Pendamping Proses Produk Halal (P3H) untuk membantu pelaku usaha dalam mengurus sertifikasi halal. Tujuan utama dari pendampingan ini adalah meningkatkan jumlah UMKM yang memperoleh sertifikasi halal, memperkuat kepercayaan konsumen, memberikan jaminan dalam proses produksi bagi para produsen, meningkatkan daya saing produk, merangsang pertumbuhan omset penjualan, membuka peluang pemasaran global, serta mematuhi ketentuan pemerintah terkait regulasi halal. Pelaksanaan program pendampingan ini terdiri dari tiga tahap, yaitu: pra pengabdian, pelaksanaan pendampingan, dan evaluasi pasca pendampingan. Kegiatan pendampingan melibatkan beberapa aspek, seperti memberikan pemahaman akan pentingnya sertifikasi halal bagi UMKM, membantu dalam pengurusan legalitas usaha melalui Nomor Induk Berusaha (NIB), dan memfasilitasi program sertifikasi halal gratis (SEHATI). Selanjutnya, pemberian NIB, sertifikat halal, serta logo halal menjadi bagian dari upaya pendampingan ini. Kegiatan ini difokuskan pada pelaku UMKM yang berlokasi di kecamatan tertentu di Kota Bengkulu. Pendampingan dilaksanakan dalam rentang waktu antara 1 Juli hingga 1 November 2023, yang melibatkan 11 pelaku usaha. Melalui kurasi dan sosialisasi self declare, sebanyak 10 UMKM memenuhi persyaratan yang ditetapkan. Hingga akhir Oktober, telah terbit 10 sertifikat halal yang dikeluarkan oleh Majelis Ulama Indonesia (MUI) berdasarkan hasil pendampingan.
Interaksi Manusia dan Lingkungan dalam Kajian Filosofis
Authors: Ratnasari Anisza , Basuki Dwisusanto Yohanes
Published: January 2024

The study of human and environmental interactions focuses on the symbiotic relationship between humans and the environment. This discussion cannot be separated from socio-cultural aspects that involve cross-disciplinary discussions, such as psychology, anthropology, sociology, social psychology, geography, ethology, and so on seek to find answers to questions about how the environment has been shaped, perceived, used and enjoyed. This study aims to explore the nature of human interaction and the environment. A qualitative method with an explanatory descriptive approach was used to find the philosophical background of human and environmental interactions. Based on the exploration, it was found that human characteristics as individuals or groups play a role in shaping their environment, there are unique mechanisms for connecting humans and the environment, and there is an influence of the environment on humans or vice versa.
Kajian Penggunaan Material Beton Ekspos pada Gedung Panjang Taman Ismail Marzuki Jakarta
Authors: Satria Widi Dwi , Damanik Novita Hillary Christy , Marda Ahmad Zaldian , Juniarto Ardiansyah , Fauzi Zulfikar Ahmad
Published: January 2024

TTaman Ismail Marzuki is an arts and culture center in Jakarta that is rich in historical value. After being revitalized, Taman Ismail Marzuki appears with a torn and modern aesthetic. Gedung Panjang is one of the buildings in Ismail Marzuki Park, which looks iconic with a piano-like appearance and looks like steps from a distance. The dominant use of exposed concrete material is used in long buildings to give the impression of a natural building and make it appear as it is. On the other hand, there is an assumption that exposed concrete is a monotonous material, dark in color, and prone to weather damage. This research aims to identify the use of exposed concrete to be able to provide a different perspective in answering the pros and cons of using exposed concrete and provide an overview of the sense of place in the building. case study research is applied using qualitative methods by identifying the long building and other architectural works of Andra Matin as a comparison. Data collection is done by literature study from relevant articles as well as visual observation through field visits and utilizing YouTube media. The results showed that the Long Building, which has the concept of openness and honesty of materials, maximizes the use of exposed concrete materials with large repetitive concrete columns and beams with high ceilings so as to give a dramatic effect to the building. The application of exposed concrete materials combined with other elements in the building gives the impression of a varied building atmosphere but remains in harmony with the monochromatic color game. Disadvantages of exposed concrete that must be anticipated such as the monotonous color of the space because it is dominated by dark colors, moisture on the concrete surface and large maintenance costs if any damage arises.
Law, Violence and Justice in Derrida’s ‘Force of Law’
Authors: Pirovolakis Eftichis
Published: January 2024

In ‘Force of Law’, Derrida’s discussion of the ‘unstable’ distinction between law and justice exemplifies the deconstructive double bind and makes this a very significant text in virtue of its juridical, political and ethical import. The first section focuses on Derrida’s deployment of the polysemous term ‘force’. ‘Force’ refers to the enforceability of the law but also to the performative and interpretative foundational violence at the moment when a new order of legality is instituted. In the second section, I argue that Derrida’s insistence on the differential relation between law and justice and on the corollary deconstructibility of the law leads to a critique of the current legal system and its axiomatics. I show that deconstruction appeals to unconditional justice in order to call for an excessive responsibility on the part of the legal system, to broaden the category of subject of law, and to have an impact on the lives of others in the margins of the established political order.
Green Synthesis and Characterization of Silver Nanoparticles from Ficus palmata Forssk. and Evaluation of their Antibacterial Activity Against Resistant Bacteria
Authors: Rehman Tayyab , Shumail Hoor , Khalid Shah , Haq Syed Inzimam Ul
Published: January 2024

In the present study, the synthesis and characterization of silver nanoparticles were carried out through a green chemistry approach. The purpose was to determine the antibacterial potential of environmentally safe nanoparticles against commonly reported resistant bacteria. The nanoparticles were synthesized using the aqueous extract of Ficus palmata Forssk. leaves. The active components present in the leaf extract acted as reducing and capping agents. The method used was easy, cost-effective, eco-friendly, and less time-consuming. To determine the shape, size, and crystalline nature, the nanoparticles underwent X-ray diffraction, and scanning electron microscopy. The synthesized nanoparticles were found to be crystalline in nature and spherical in shape, with an average diameter of about 30 nm. The antibacterial activity of the silver nanoparticles (20 µl) was evaluated against a range of antibiotic-resistant bacteria using standard microbiological procedures. They were found to be effective against MRSA, Bacillus subtilis, Staphylococcus aureus, and Klebsiella pneumonia.
Z Kuşağının Türkiye’nin Dış Politikalarına Yönelik Yaklaşımı Üzerine Bir Alan Araştırması
Authors: Dilekli Karatay Zeynep , Koyuncu Ahmet Ayhan
Published: January 2024

Toplumsal bir gerçeklik olarak nitelendirilen kuşak, geçmişten günümüze varlığını koruyarak gelebilmeyi başarmış toplumsal bir olgudur. Literatürde Sessiz Kuşak, Bebek Patlaması Kuşağı, X, Y ve Z kuşakları olmak üzere ele alınan beş temel kuşak bulunmaktadır. Z kuşağı sonrasında doğanlar ise Alfa olarak kabul edilmeye başlanmıştır. Z kuşağı 1996-2010 yılları arasında doğanları kapsayan bir kuşaktır. Z kuşağı bireyleri günün en az üç saatini sosyal medya kullanarak geçirmektedir. Bu kuşak bireyleri gündemi daha çok internet ve sosyal medya platformlarından takip etmektedir. Çoğunluğunun eğitim hayatı devam eden bu kuşak bireyleri gelecekleri adına da oldukça kaygılıdırlar. Z kuşağının dış politikalara nasıl yaklaştığını ortaya koymayı amaçlaması nedeniyle çalışmada yaklaşımlara ilişkin nedensel yorumlar sınırlı tutulmuştur. Çalışmadan elde edilen veriler Z kuşağının dış politikalara ilgisiz olmadığını göstermektedir. Z kuşağının dış politika yaklaşımlarında özellikle yakınlık duydukları siyasi partinin ve bulunulan bölgenin etkili olduğu sonucuna ulaşılmıştır. Fakat çeşitli demografik değişkenler Z kuşağının yaklaşımlarının farklılaşmasında etkili olmamıştır. Bu sonuç Z kuşağının benzer özellikler taşıyan en küresel kuşak olduğunu da doğrulamaktadır. Araştırma evrenini Türkiye’deki Z kuşağı, örneklemini ise 18-27 yaş aralığında olan toplam 698 Z kuşağı bireyi oluşturmaktadır. Veriler anket tekniği ile toplanmış ve bilimsel olarak analiz edilmiştir.
Type 1 diabetes mellitus in the context of high levels of rural deprivation: differences in demographic and anthropometric characteristics between urban and rural cases in NW Ethiopia
Authors: Balcha Shitaye A. , Phillips David I. , Trimble Elisabeth R.
Published: January 2024

Background

While there is increasing evidence for an altered clinical phenotype of Type 1 diabetes in several low-and middle-income countries, little is known about urban-rural differences and how the greater poverty of rural environments may alter the pattern of disease.

Objective

Investigation of urban-rural differences in demographic and anthropometric characteristics of type 1 diabetes in a resource-poor setting.

Research design and methods

Analysis of a unique case register, comprising all patients (rural and urban) presenting with Type 1 diabetes over a 20 yr. period in a poor, geographically defined area in northwest Ethiopia. The records included age, sex, place of residence, together with height and weight at the clinical onset.

Results

A total of 1682 new cases of Type 1 diabetes were registered with a mean age of onset of 31.2 (SD 13.4) yr. The patients were thin with 1/3 presenting with a body mass index (BMI) <17kg/m2. There was a striking male predominance of cases when clinical onset was between 20 and 35 yr., this was more marked in the very poor rural dwellers compared to the urban population. While most patients with Type 1 diabetes presented with low BMIs and reduced height, stunting preferentially affected rural men.

Conclusions

These data have led to the hypothesis that complex interactions among poor socioeconomic conditions in early life affect both pancreatic function and the development of autoimmunity and provide a possible explanation of the unusual phenotype of Type 1 diabetes in this very poor community.
Transcriptomic analysis reveals the lipid metabolism-related gene regulatory characteristics and potential therapeutic agents for myocardial ischemia-reperfusion injury
Authors: Wu Jiahe , Cai Huanhuan , Hu Xiaorong , Wu Wei
Published: January 2024

Background

Impaired energy balance caused by lipid metabolism dysregulation is an essential mechanism of myocardial ischemia-reperfusion injury (MI/RI). This study aims to explore the lipid metabolism-related gene (LMRG) expression patterns in MI/RI and to find potential therapeutic agents.

Methods

Differential expression analysis was performed to screen the differentially expressed genes (DEGs) and LMRGs in the MI/RI-related dataset GSE61592. Enrichment and protein-protein interaction (PPI) analyses were performed to identify the key signaling pathways and genes. The expression trends of key LMRGs were validated by external datasets GSE160516 and GSE4105. The corresponding online databases predicted miRNAs, transcription factors (TFs), and potential therapeutic agents targeting key LMRGs. Finally, the identified LMRGs were confirmed in the H9C2 cell hypoxia-reoxygenation (H/R) model and the mouse MI/RI model.

Results

Enrichment analysis suggested that the “lipid metabolic process” was one of the critical pathways in MI/RI. Further differential expression analysis and PPI analysis identified 120 differentially expressed LMRGs and 15 key LMRGs. 126 miRNAs, 55 TFs, and 51 therapeutic agents were identified targeting these key LMRGs. Lastly, the expression trends of Acadm, Acadvl, and Suclg1 were confirmed by the external datasets, the H/R model and the MI/RI model.

Conclusion

Acadm, Acadvl, and Suclg1 may be the key genes involved in the MI/RI-related lipid metabolism dysregulation; and acting upon these factors may serve as a potential therapeutic strategy.
Triglyceride-glucose index is a predictor of the risk of prostate cancer: a retrospective study based on a transprostatic aspiration biopsy population
Authors: Zhou Yijie , Li Tianqi , Muheiyati Guliman , Duan Yajun , Xiao Songtao , Gao Yi , Tao Ning , An Hengqing
Published: January 2024

Background

Current research suggests that prostate cancer (PCa), one of the most common cancers in men, may be linked to insulin resistance (IR).Triglyceride-glucose index (TyG index) was made for a marker of insulin resistance. We investigated the relationship between the TyG index and the risk of PCa.

Objective

To assess the correlation and dose-response relationship between TyG index and prostate cancer.

Method

Retrospectively, 316 patients who required prostate biopsy puncture in the First Affiliated Hospital of Xinjiang Medical University from March 2017 to July 2021 were collected, and the relationship between factors such as the TyG index and prostate cancer was analyzed by Logistic regression model combined with a restricted cubic spline.

Results

(1) The differences in age, initial PSA and TyG index between the two groups were statistically significant; (2) Logistic regression results showed that the risk of prostate cancer in the highest quartile of the TyG index (Q4) was 3.387 times higher than that in the lowest quartile (Q1) (OR=3.387,95% CI [1.511,7.593], P=0.003); (3) The interaction results showed a significant interaction between the TyG index Q4 group and age with the risk of developing prostate cancer (P for interaction<0.001). (4) The results of the restricted cubic spline showed a linear dose-response relationship between the TyG index and the risk of prostate cancer; (5) The Receiver operating characteristic (ROC) curve results showed that the area under the curve (AUC) of the TyG index combined with initial PSA and age was 0.840, with a sensitivity and specificity of 62.5% and 93.3%, respectively.

Conclusion

TyG index and age are risk factors for prostate cancer, and the interaction between the TyG index and different risk factors may increase the risk of prostate cancer. TyG index has some predictive value for the risk of prostate cancer, and the risk of prostate cancer can be reduced by controlling the levels of blood lipids and blood glucose.
Exploring the evolving function of soluble intercellular adhesion molecule-1 in junction dynamics during spermatogenesis
Authors: Xiao Xiang , Han Yating , Li Qin , Zheng Dongwang , Cheng C. Yan , Ni Ya
Published: January 2024

Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane glycoprotein expressed on immune, endothelial, and epithelial cells. Its ectodomain can be proteolytically cleaved to release a circulating soluble form called sICAM-1. Clinical studies demonstrate sICAM-1 is upregulated in various diseases and associated with disease severity. Research has identified sICAM-1 as a regulator of the blood-testis barrier (BTB) and spermatogenesis. Overexpression of sICAM-1 weakened the BTB in vitro and in vivo, downregulated junction proteins including N-cadherin, γ-catenin, and connexin 43, and caused germ cell loss. This contrasts with barrier-strengthening effects of membrane-bound ICAM-1. sICAM-1 may act as a molecular switch enabling germ cells to open BTB and Sertoli-germ cell adhesion for transport across the seminiferous epithelium. While the mechanism remains unclear, reduced SRC family kinase (SFK) signaling was observed following sICAM-1 overexpression. SRC promotes BTB protein endocytosis and degradation, influences cytoskeletal dynamics, and affects cell polarity. As sICAM-1 overexpression phenocopies SRC inhibition, SRC may operate downstream of sICAM-1 in regulating BTB dynamics and spermatogenesis. Investigating sICAM-1’s structure-function regions and downstream targets will elucidate the molecular mechanisms of junction disruption. This knowledge could enable strategies targeting sICAM-1/SRC to modulate BTB permeability and treat male infertility or diseases involving endothelial/epithelial barrier dysfunction.
First-line treatment with sodium-glucose cotransporter 2 inhibitors and glucagon-like peptide-1 receptor agonists in type 2 diabetic population at low risk of cardiovascular disease: a meta-analysis
Authors: Deng Rui , Mei Kaibo , Song Tiangang , Huang Jinyi , Wu Yifan , Yu Peng , Yan Zhiwei , Liu Xiao
Published: January 2024

Background

The benefit of first-line use of sodium-dependent glucose transport 2 inhibitors (SGLT2i) and glucagon-like peptide-1 receptor agonists (GLP-1RAs) in type 2 diabetes mellitus (T2DM) with low risk of cardiovascular diseases are not clear.

Methods

PubMed, EMBASE and Cochrane Library databases were searched to identify eligible randomized controlled trials. We used the odds ratio (OR) and mean difference (MD) and the corresponding 95% confidence interval (CI) to assess the dichotomous and continuous variable, respectively.

Results

Thirteen studies involving 2,885 T2DM at low risk of cardiovascular diseases were included. Compared to placebo, first line use of SGLT2i significantly reduced glycosylated hemoglobin type A1C (HbA1c) (MD: -0.72), weight (MD: -1.32) and fasting plasma glucose (FPG) (MD: -27.05) levels. Compared with metformin, SGLT2i reduced body weight (MD: -1.50) and FPG (MD: -10.13) more effectively, with similar reduction for HbA1c (MD: -0.05). No significant increased safety adverse was found for SGLT2i, including nasopharyngitis (OR: 1.07), urinary tract infection (OR: 2.31), diarrhea (OR: 1.18) and hypoglycemia (OR: 1.06). GLP-1RAs significantly reduced HbA1c (MD: -1.13), weight (MD: -2.12) and FPG (MD: -31.44) levels as first-line therapy compared to placebo. GLP-1RAs significantly increased occurrence of diarrhea (OR: 2.18), hypoglycemia (OR: 3.10), vomiting (OR: 8.22), and nausea (OR: 4.41).

Conclusion

First line use of SGLT2i and GLP-1RAs is effective in reducing HbA1c, weight, and FPG levels in T2DM patients at low risk for cardiovascular disease. SGLT2i may be superior to metformin in controlling body weight and FPG. GLP-1RAs may increase the occurrence of diarrhea, hypoglycemia, vomiting, and nausea.

Systematic review registration

PROSPERO (International Prospective Register of Systematic Reviews. https://www.york.ac.uk/inst/crd, CRD42022347233).
An advanced fractal-based well testing model capturing fracture complexity in low permeability tight gas reservoirs
Authors: Wang Kun , Li Li , Xie Mingying , Dai Jianwen , Feng Shasha , Li Min , Huang Ruijie , Liu Siyu
Published: January 2024

The study of heterogeneous and complex oil and gas reservoirs poses a significant challenge due to the problem of unstable seepage flow. This research investigates the non-linear seepage flow patterns in such reservoirs, with a particular focus on ultra-low permeability tight gas reservoirs, using fractal theory. By analysing the scale invariance in the seepage flow of ultra-low permeability tight gas reservoirs, we have derived a fractal geometric expression based on the capillary pressure curve. This expression was integrated with mercury intrusion porosimetry to study the fractal dimension of ultra-low permeability reservoirs. Our experimental results indicate the presence of fractal characteristics in ultra-low permeability tight gas reservoirs. Based on this, we have investigated the fractal nature of matrix porosity and fractures and their effect on the stress sensitivity of ultra-low permeability tight gas reservoirs. This led to the development of a new fractal model for seepage flow in these reservoirs, which takes into account the non-Darcy flow behaviour of gas in nanoscale pores. The mathematical model was simplified using point source solutions and regular perturbation methods. The analysis shows that the fractal parameters of the matrix and fractures, together with the adsorption coefficients and the permeability modulus, have a significant influence on the dynamic pressure behaviour. The effects of slip and diffusion coefficients and stress sensitivity on the dynamics were also investigated. The pressure dynamics curves of ultra-low permeability tight gas reservoirs show distinct differences from those of homogeneous reservoirs and conventional dual porosity reservoirs. This study not only reveals the unique dynamic pressure characteristics of ultra-low permeability tight gas reservoirs, but also shows that the traditional reservoir models are, under certain conditions, special cases of the model proposed in this paper.
Analysis and quantitative evaluation of wind turbine frequency support capabilities in power systems
Authors: He Hongliang , Xiao Huangqing , Yang Ping
Published: January 2024

With the rapid development of photovoltaic and wind power, the penetration rate of renewable energy in power system is gradually increasing. This escalation poses a challenge as it results in a continuous reduction in the inertia and damping of the power grid, accentuating the issue of frequency stability in power systems. It is one of the effective measures to deal with this risk that renewable energy sources such as wind turbines actively provide frequency support for power grid. This paper carries out research into the examination of wind turbines’ capacity to contribute to system frequency support, considering two aspects: inertia support and primary frequency regulation capabilities. Subsequently, the frequency control method of the wind turbine support system is analyzed, emphasizing the roles of rotor kinetic energy control and power reserve control in facilitating frequency support. The evaluation of the transient frequency support capability of wind turbines is introduced, incorporating factors such as control methods, controller parameters, and the duration of transient frequency support. Key metrics, including accumulated energy and frequency change rate indices during the transient frequency support stage, are proposed to quantitatively assess the transient frequency support capability of wind turbines. These indices provide a comprehensive framework for the quantitative evaluation of wind turbine transient frequency support capabilities.
Lung single-cell RNA profiling reveals response of pulmonary capillary to sepsis-induced acute lung injury
Authors: Yang Ruhao , Zheng Ting , Xiang Hongyu , Liu Menglin , Hu Ke
Published: January 2024

Background

Sepsis-induced acute lung injury (ALI) poses a significant threat to human health. Endothelial cells, especially pulmonary capillaries, are the primary barriers against sepsis in the lungs. Therefore, investigating endothelial cell function is essential to understand the pathophysiological processes of sepsis-induced ALI.

Methods

We downloaded single-cell RNA-seq expression data from GEO with accession number GSE207651. The mice underwent cecal ligation and puncture (CLP) surgery, and lung tissue samples were collected at 0, 24, and 48 h. The cells were annotated using the CellMarker database and FindAllMarkers functions. GO enrichment analyses were performed using the Metascape software. Gene set enrichment Analysis (GSEA) and variation Analysis (GSVA) were performed to identify differential signaling pathways. Differential expression genes were collected with the “FindMarkers” function. The R package AUCell was used to score individual cells for pathway activities. The Cellchat package was used to explore intracellular communication.

Results

Granulocytes increased significantly as the duration of endotoxemia increased. However, the number of T cells, NK cells, and B cells declined. Pulmonary capillary cells were grouped into three sub-clusters. Capillary-3 cells were enriched in the sham group, but declined sharply in the CLP.24 group. Capillary-1 cells peaked in the CLP.24 group, while Capillary-2 cells were enriched in the CLP.48 group. Furthermore, we found that Cd74+ Capillary-3 cells mainly participated in immune interactions. Plat+ Capillary-1 and Clec1a+ Capillary-2 are involved in various physiological processes. Regarding cell-cell interactions, Plat+ Capillary-1 plays the most critical role in granulocyte adherence to capillaries during ALI. Cd74+ Capillary cells expressing high levels of major histocompatibility complex (MHC) and mainly interacted with Cd8a+ T cells in the sham group.

Conclusion

Plat+ capillaries are involved in the innate immune response through their interaction with neutrophils via ICAM-1 adhesion during endotoxemia, while Cd74+ capillaries epxressed high level of MHC proteins play a role in adaptive immune response through their interaction with T cells. However, it remains unclear whether the function of Cd74+ capillaries leans towards immunity or tolerance, and further studies are needed to confirm this.
TRIM56: a promising prognostic immune biomarker for glioma revealed by pan-cancer and single-cell analysis
Authors: Wang Bingcheng , Wang Zhihai , Li Yuchen , Shang Zehan , Liu Zihao , Fan Hao , Zhan Rucai , Xin Tao
Published: January 2024

Tripartite-motif 56 (TRIM56) is a member of the TRIM family, and was shown to be an interferon-inducible E3 ubiquitin ligase that can be overexpressed upon stimulation with double-stranded DNA to regulate stimulator of interferon genes (STING) to produce type I interferon and thus mediate innate immune responses. Its role in tumors remains unclear. In this study, we investigated the relationship between the expression of the TRIM56 gene and its prognostic value in pan-cancer, identifying TRIM56 expression as an adverse prognostic factor in glioma patients. Therefore, glioma was selected as the primary focus of our investigation. We explored the differential expression of TRIM56 in various glioma subtypes and verified its role as an independent prognostic factor in gliomas. Our research revealed that TRIM56 is associated with malignant biological behaviors in gliomas, such as proliferation, migration, and invasion. Additionally, it can mediate M2 polarization of macrophages in gliomas. The results were validated in vitro and in vivo. Furthermore, we utilized single-cell analysis to investigate the impact of TRIM56 expression on cell communication between glioma cells and non-tumor cells. We constructed a multi-gene signature based on cell markers of tumor cells with high TRIM56 expression to enhance the prediction of cancer patient prognosis. In conclusion, our study demonstrates that TRIM56 serves as a reliable immune-related prognostic biomarker in glioma.
Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma
Authors: Yu Tiantian , Xu-Monette Zijun Y. , Lagoo Anand , Shuai Wen , Wang Bangchen , Neff Jadee , Carrillo Luis F. , Carlsen Eric D. , Pina-Oviedo Sergio , Young Ken H.
Published: January 2024

Introduction

Our previous studies have demonstrated that tumor-infiltrating lymphocytes (TILs), including normal B cells, T cells, and natural killer (NK) cells, in diffuse large B-cell lymphoma (DLBCL) have a significantly favorable impact on the clinical outcomes of patients treated with standard chemoimmunotherapy. In this study, to gain a full overview of the tumor immune microenvironment (TIME), we assembled a flow cytometry cohort of 102 patients diagnosed with DLBCL at the Duke University Medical Center.

Methods

We collected diagnostic flow cytometry data, including the proportion of T cells, abnormal B cells, normal B cells, plasma cells, NK cells, monocytes, and granulocytes in fresh biopsy tissues at clinical presentation, and analyzed the correlations with patient survival and between different cell populations.

Results

We found that low T cell percentages in all viable cells and low ratios of T cells to abnormal B cells correlated with significantly poorer survival, whereas higher percentages of normal B cells among total B cells (or high ratios of normal B cells to abnormal B cells) and high percentages of NK cells among all viable cells correlated with significantly better survival in patients with DLBCL. After excluding a small number of patients with low T cell percentages, the normal B cell percentage among all B cells, but not T cell percentage among all cells, continued to show a remarkable prognostic effect. Data showed significant positive correlations between T cells and normal B cells, and between granulocytes and monocytes. Furthermore, we constructed a prognostic model based on clinical and flow cytometry factors, which divided the DLBCL cohort into two equal groups with remarkable differences in patient survival and treatment response.

TILs, including normal B cells, T cells, and NK cells, are associated with favorable clinical outcomes in DLBCL, and flow cytometry capable of quantifying the TIME may have additional clinical utility for prognostication.
Case report: Giant lymph node metastases: a new opportunity for cancer radioimmunotherapy?
Authors: Bian Yuan , Zhang Zhenhua , Deng Xiangyu , Wen Qinglian , Li Dan
Published: January 2024

Background

Despite the significant progress made in radiotherapy and chemotherapy for the treatment of cervical cancer, patients with lymph node metastasis still have a poor prognosis. It is widely accepted that lymph node metastasis plays a crucial role in the spread of cancer to other organs and is considered an independent factor in predicting a poor prognosis. However, recent research suggests that the importance of lymph nodes in tumor therapy needs to be reevaluated, as preserving the integrity of lymph nodes before immunotherapy can enhance treatment effectiveness.

Case presentation

In this report, we present two cases of advanced cervical cancer patients with giant metastatic lymph node lesions in the neck. These patients were effectively treated with a combination of local radiotherapy and immunotherapy after conventional chemoradiotherapy had failed. The combination therapy resulted in significant clinical improvements, with patient 1 achieving over 12 months of progression-free survival (PFS) and patient 2 maintaining sustained remission for an impressive 24 months.

Conclusions

The combination of local radiotherapy and immunotherapy shows promise as a viable treatment option for cervical cancer patients with distant lymph node metastasis, and the giant lymph node metastases may play an important role in this process, which might provide a new opportunity for cancer radioimmunotherapy.
Understanding the caring network of podoconiosis patients in Rwanda: a qualitative study
Authors: Risat Md Ilias Kamal , Davey Gail , Mugume Peter , Zaman Shahaduz
Published: January 2024

Introduction

Globally, a total of almost 4 million people live with podoconiosis in 32 potentially endemic countries including Rwanda. Podoconiosis is a non-infectious geochemical disease that causes massive swelling of the lower leg and is caused by long-term exposure to red clay soil found in tropical highland areas. The disease is a disabling neglected tropical disease (NTD) and is associated with profound stigma, discrimination and comorbid mental health conditions. Treatment interventions are commonly known as morbidity management and disability prevention. Both biomedical and traditional treatments are used by affected people. However, understandings informed by the social sciences of care in the context of NTDs are largely unexplored. This study explored the perspectives and experiences of care among care receivers (podoconiosis patients) and caregivers (family members, traditional healers) in the district of Huye, Rwanda.

Method

The study used qualitative methods including seventeen InDepth Interviews (eleven patients, two traditional healers, two care professionals, and two family members) and participant observation in a health centre and patients’ houses.

Result

A caring network was found amongst the podoconiosis patients, their family members, care professionals and traditional healers. Caring network is not only about the medical treatments, but also about the caring relationship amongst them.

Discussion

Using notions of ‘Network’ and ‘Collectives’, from the care ethics literature the study shows that in addition to care work requiring professional know-how, it is also about the relationships between patients, their families, traditional healers, and biomedical care professionals.
Culture-based and culture-independent approach for the study of the methanogens and obligate anaerobes from different landfill sites
Authors: Prakash Om , Dewala Sahab Ram , Nimonkar Yogesh , Patil Shalaka K. , Chauhan Ashvini , Yadav Amit , Dhotre Dheeraj P. , Ranade Dilip R.
Published: January 2024

The landfill is a cheap way of solid waste management in developing countries. The majority of landfills are non-sanitary and work as open garbage dumping sites and pose threats to public and environmental health. Therefore, an in-depth understanding of the chemistry and microbiology of landfills is imperative to develop the right policies for landfill management. In the current study, we investigated the chemistry and microbiology of three Indian landfill sites using culture-based and culture-independent molecular approaches. Our data indicate that the nature of landfills varies from site to site in terms of chemistry, pollutants, and pathogens. We also enriched and cultivated three methanogens using an optimized medium and constructed two high-quality draft genomes from enriched microbiomes using metagenome-assembled genome approaches. The phylogenomic study of one draft genome showed the highest 93% sequence similarity with members of Methanomassiliicoccaceae and was always enriched with Acholoplasma and Anaerohalosphaera lusitana. Despite all the efforts, we did not isolate it in pure culture and hypothesized that for the cultivation of some not-yet-cultured methanogen, the presence of other organisms plays an important role, and their syntrophic interaction must be discerned for its successful cultivation in the future. Co-cultivation of amino acid-degrading organisms indicates that their co-culture can assist in boosting the growth of methanogens. In addition, our data indicated that landfill leachate contains a heavy load of pollutants and treatment is a must before discharge in nature or use in irrigation or biofertilizer.
Causal relationship between gut microbiota and hidradenitis suppurativa: a two-sample Mendelian randomization study
Authors: Liu Chengling , Liu Xingchen , Li Xin
Published: January 2024

Background

Accumulating evidence suggests that alterations in gut microbiota composition are associated with the hidradenitis suppurativa (HS). However, the causal association between gut microbiota and HS remain undetermined.

Methods

We performed a bidirectional two-sample Mendelian randomization (MR) analysis using genome-wide association study summary data of gut microbiota and hidradenitis suppurativa from the MiBioGen consortium which concluded 18,340 individuals analyzed by the MiBioGen Consortium, comprising 211 gut microbiota. HS data were acquired from strictly defined HS data collected by FinnGenbiobank analysis, which included 211,548 European ancestors (409 HS patients, 211,139 controls). The inverse variance weighted method (IVW), weighted median (WME), simple model, weighted model, weighted median, and MR-Egger were used to determine the changes of HS pathogenic bacterial taxa, followed by sensitivity analysis including horizontal pleiotropy analysis. The MR Steiger test evaluated the strength of a causal association and the leave-one-out method assessed the reliability of the results. Additionally, a reverse MR analysis was carried out to seek for possible reverse causality.

Results

By combining the findings of all the MR steps, we identified four causal bacterial taxa, namely, Family XI, Porphyromonadaceae, Clostridium innocuum group and Lachnospira. The risk of HS might be positively associated with a high relative abundance of Clostridium innocuum group (Odds ratio, OR 2.17, p = 0.00038) and Lachnospira (OR 2.45, p = 0.017) but negatively associated with Family XI (OR 0.67, p = 0.049) and Porphyromonadaceae (OR 0.29, p = 0.014). There were no noticeable outliers, horizontal pleiotropy, or heterogeneity. Furthermore, there was no proof of reverse causation found in the reverse MR study.

Conclusion

This study indicates that Clostridium innocuum group and Lachnospira might have anti-protective effect on HS, whereas Family XI and Porphyromonadaceae might have a protective effect on HS. Our study reveals that there exists a beneficial or detrimental causal effect of gut microbiota composition on HS and offers potentially beneficial methods for therapy and avoidance of HS.
Acupuncture therapy for Parkinson’s disease: a case report demonstrating symptomatic improvement without medication
Authors: Lei Suying , Liu Qing , Leong IanI , Fan Jingqi , Tsang YauKeung , Liu Xin , Xu Xiaoyan , Zhuang Lixing
Published: January 2024

Background

Parkinson’s disease (PD) often necessitates immediate medical intervention following diagnosis. In recent years, there has been a noticeable increase in clinical investigations assessing the efficacy of acupuncture in PD, with many studies reporting positive outcomes. Ethical guidelines commonly endorse pharmaceutical therapies for PD, leading ongoing research to combine acupuncture with standard drug-based treatments. At present, there is a conspicuous absence of dedicated clinical research exclusively examining the independent impact of acupuncture on PD treatment.

Case

In a clinical observation, we documented a case involving a 75-year-old male displaying progressive, characteristic PD symptoms, including evident limb tremors, rigidity, bradykinesia, fatigue, and additional non-motor symptoms. The patient received a confirmed diagnosis of PD. Due to the refusal of the patient to take medication, we exclusively administered acupuncture therapy. The outcomes indicated a noteworthy enhancement in the clinical symptoms of the patient solely through acupuncture intervention.

Conclusion

This case affirms that using acupuncture in isolation significantly improved both the motor and non-motor symptoms in the patient. Acupuncture could potentially serve as an alternative therapy for patients who decline or are intolerant to anti-Parkinson drugs. However, further studies are needed to assess its long-term efficacy. This case report obtained approval from the Ethics Committee of the First Affiliated Hospital of Guangzhou University of Chinese Medicine (Ethics number: K-2023-127).
Quantified fat fraction as biomarker assessing disease severity in rare Charcot–Marie–Tooth subtypes
Authors: Sun Xingwen , Liu Xiaoxuan , Zhao Qiang , Zhang Lihua , Yuan Huishu
Published: January 2024

Objective

Charcot–Marie–Tooth (CMT) disease is the most common inherited neuromuscular disorder. Multi-echo Dixon MRI technique is a highly sensitive method for quantifying muscle fatty infiltration, which may provide excellent value for the assessment of CMT. Due to the rareness of the disease, its use in CMT disease has been rarely evaluated, especially in subtypes.

Methods

Thirty-four CMT1 patients, 25 CMT2 patients, and 10 healthy controls were recruited. All of the recruited CMT1 patients are CMT1A with PMP22 duplication. Among CMT2 patients, 7 patients are CMT2A with MFN2 mutation, and 7 patients have SORD mutations. Multi-echo Dixon MRI imaging was performed. The fat fractions (FFs) of 5 muscle compartments of the leg were measured at proximal, middle, and distal levels by two specialized musculoskeletal radiologists. Comparisons between CMT1, CMT2, and genetically defined subtypes were conducted.

Results

A proximal-distal gradient (27.6 ± 15.9, 29.9 ± 19.7, and 40.5 ± 21.4, p = 0.015) with a peroneal predominance (p = 0.001) in fat distribution was observed in CMT1. Significant differences in the soleus muscle FFs at proximal (19.1 ± 14.7 vs. 34.8 ± 25.1, p = 0.034) and medial levels (23.5 ± 21 vs. 38.0 ± 25.6, p = 0.044) were observed between CMT1 and CMT2 patients. Between PMP2 duplication and MFN2 mutation group, a significant difference in the soleus muscle FF was also observed (23.5 ± 21.0 vs. 54.7 ± 20.2, p = 0.039). Prominent correlations of calf muscle FFs with functional scores were observed.

Discussion

Multi-echo Dixon MRI imaging is a valuable tool for assessing disease severity in CMT. The difference in patterns of fatty infiltration of CMT subtypes is first reported, which could provide references when making targeted training plans.
B-Mode ultrasound imaging in diagnosing carpal tunnel syndrome: an auxiliary diagnostic tool for hand surgeons
Authors: Chen Qiang , Zou Xiaodi , Xia Yanting , Hu Yingnan , Chen Congxian , Zheng Ping
Published: January 2024

Objective

The purpose of this article is to explore the effectiveness of B-Mode ultrasound as an auxiliary diagnostic tool for carpal tunnel syndrome (CTS). It aims to demonstrate the advantages of B-Mode ultrasound, including its non-invasive nature and its ability to provide real-time imaging, in localizing nerve compression and predicting postoperative outcomes.

Methods

The study included 40 patients who were subjected to preoperative B-ultrasonography. The approach focused on evaluating the consistency of B-Mode ultrasound results with intraoperative findings. It also assessed the importance of employing standardized imaging techniques and emphasized the need for cooperation between hand surgeons and sonographers for accurate diagnosis.

Results

B-Mode ultrasound findings in the study were consistent with intraoperative observations, indicating its reliability. Additionally, B-Mode ultrasound was able to identify other anatomical abnormalities within the carpal canal that may contribute to CTS symptoms, such as persistent median arteries, median nerve bifurcation, and space-occupying lesions like cysts and tumors.

Conclusion

The article concludes that B-Mode ultrasound should be considered a valuable supplementary diagnostic tool for CTS, particularly in instances where clinical signs and electrophysiological studies do not offer clear results. However, it should not replace established diagnostic methods for CTS.