bluuebunny/crossref_metadata_2025 huggingface dataset:
ITRAQ Based Proteomics Reveals the Potential Mechanism of Placental Injury Induced by Prenatal Stress
Authors: Li Yujie , Hou Junlin , Yang Liping , Zhang Tong , Jiang Yu , Du Zhixing , Ma Huizi , Li Gai , Zhu Jianghui , Chen Ping
Published: September 2024

Maternal stress experienced during prenatal development is recognized as a significant risk factor for neurodevelopmental and neuropsychiatric disorders across the offspring’s lifespan. The placental barrier serves a crucial function in safeguarding the fetus from detrimental exposures during gestation. However, previous investigations have not yet comprehensively elucidated the extensive connections between prenatal stress and the expression of placental proteins. In this study, we used iTRAQ-based quantitative proteomics to elucidate the placental adaptive mechanisms of pregnant rats in response to fear-induced stress. Our results showed that during pregnancy, exposure to fear-induced stress led to a pathological hypercoagulable state in the mother’s body. Placental circulation was also disrupted, significantly reducing placental efficiency and blood oxygen saturation in newborn rats. Proteomic analyses showed that most of the DEPs were annotated to the PI3K-Akt and ECM-receptor interaction signaling pathway. In addition, the expressions of CDC37, HSP90β, AKT, p-AKT and p-mTOR were down-regulated significantly in the placenta. Our results demonstrated that prenatal fear-induced stress led to inhibition of the cellular signal transduction of placental PI3K/AKT/mTOR, which affected biological processes such as rRNA processing, translation, protein folding, protein stability, and oxygen transport in the placenta. These abnormalities in biological functions could potentially damage the barrier function of the placenta and thereby result in abnormal development in the offspring.
Iodine Rearrangements of Tetraallylsilane and Synthesis of Silicon-Stereogenic Organosilanes
Authors: Tan Elliott D. , Wier Kerry E. , O’Neil Gregory W.
Published: September 2024

Tetraallylsilane can undergo either a mono or double rearrangement when treated with iodine (I2). The extent of rearrangement depends on the equivalents of I2 used, where 1 equivalent gives high yields of mono-rearranged products and excess (e.g., 3 equivalents) causes double rearrangement to occur. This transformation can be applied to the synthesis of potentially valuable silicon-stereogenic organosilanes.
Real-Time Identification and Nonlinear Control of a Permanent-Magnet Synchronous Motor Based on a Physics-Informed Neural Network and Exact Feedback Linearization
Authors: Velarde-Gomez Sergio , Giraldo Eduardo
Published: September 2024

This work proposes a novel method for the real-time identification and nonlinear control of a permanent-magnet synchronous motor (PMSM) based on a Physics-Informed Neural Network (PINN) and the exact feedback linearization approach. The proposed approach is presented in a direct-quadrature framework, where the quadrature current and the rotational speed are selected as outputs and the direct and quadrature voltages are selected as inputs. A nonlinear difference equation is selected to describe the physical dynamics of the PMSM, and a PINN is designed based on the aforementioned structure. A simplified training scheme is designed for the PINN based on a least-squares structure to facilitate online training in real time. A nonlinear controller based on exact feedback linearization is designed by considering the nonlinear model of the system identified based on the PINN. Therefore, the proposed approach involves identification and control in real time, where the PINN is trained online. In order to track the reference for the rotational speed, a nonlinear controller with integral action based on exact feedback linearization is designed based on a linear quadratic regulator. As a result, the proposed approach can be used to identify the system to be controlled in real time, and it is able to track any small change in the real model; in addition, it is robust to both external and internal disturbances, such as variations in torque load and resistance. The proposed approach is evaluated through simulation and using a real PMSM, and the results of reference tracking are evaluated under disturbances. The identification performance is evaluated by using a Taylor diagram under closed-loop and open-loop structures, where ARX and NARX structures are used for comparison. It is thereby verified that this novel proposed control approach involving a PINN-based model can adequately track the dynamics of a PMSM system, where the performance of the proposed nonlinear control is maintained even when using the identified model based on the PINN.
Failure Probability-Based Optimal Seismic Design of Reinforced Concrete Structures Using Genetic Algorithms
Authors: Bojórquez Juan , Bojórquez Edén , Leyva Herian , Barraza Manuel
Published: September 2024

Artificial intelligence (AI) has enabled several optimization techniques for structural design, including machine learning, evolutionary algorithms, as in the case of genetic algorithms, reinforced learning, deep learning, etc. Although the use of AI for weight optimization in steel and concrete buildings has been extensively studied in recent decades, multi-objective optimization for reinforced concrete (RC) and steel buildings remains challenging due to the difficulty in establishing independent objective functions and obtaining Pareto fronts. The well-known Non-Dominated Sorting Genetic Algorithm II (NSGA-II) is an efficient genetic algorithm approach for multi-objective optimization. In this work, the NSGA-II approach is considered for the multi-objective structural optimization of three-dimensional RC buildings subjected to earthquakes. For the objective of this study, two function objectives are considered: minimizing total cost and the probability of structural failure, which are obtained via several nonlinear seismic analyses of the RC buildings. Beams and columns’ cross-sectional dimensions are selected as design variables, and the Mexican Building Code (MBC) specifications are imposed as design constraints. Pareto fronts are obtained for two RC-framed buildings located in Mexico City (soft soil sites), which demonstrate the efficiency and accuracy of NSGA-II for structural optimization.
Characteristics of a Spray-Dried Porcine Blood Meal for Aedes aegypti Mosquitoes
Authors: Weaver Alexander R. , Rajagopal Nagarajan R. , Pereira Roberto M. , Koehler Philip G. , MacIntosh Andrew J. , Baldwin Rebecca W. , Batich Christopher D.
Published: September 2024

Research into mosquito-borne illnesses faces hurdles because feeding fresh animal blood to rear female mosquitoes presents logistical, economic, and safety challenges. In this study, a shelf-stable additive (spray-dried porcine blood; SDPB) hypothesized to supply accessible hemoglobin was evaluated within an alternative meal (AM) containing whey powder and PBS for rearing the yellow fever mosquito Aedes aegypti. LC–MS/MS proteomics, microbial assays, and particle reduction techniques confirmed and characterized the functionality of hemoglobin in SDPB, while engorgement, fecundity, egg viability, and meal stability bioassays assessed AM performance. Chemical assays supported hemoglobin as the phagostimulant in SDPB with aggregates partially solubilized in the AM that can be more accessible via particle reduction. Unpaired two-tailed t-tests indicate that the AM stimulates oogenesis (t11 = 13.6, p = 0.003) and is stable under ambient (1+ y; t12 = 0.576, p = 0.575) and aqueous (14 d; t12 = 0.515, p = 0.639) conditions without decreasing fecundity. Egg hatch rates for the ninth generation of AM-reared Ae. aegypti were 50–70+%. With further development, this meal may serve as a platform for mass rearing or studying effects of nutritional additives on mosquito fitness due to its low cost and stability. Future work may examine tuning spray drying parameters and resulting impacts on hemoglobin agglomeration and feeding.
Mosquito Feeding Habits in Coastal French Guiana: Mammals in the Crosshairs?
Authors: Guidez Amandine , Tirera Sourakhata , Talaga Stanislas , Lacour Guillaume , Carinci Romuald , Darcissac Edith , Donato Damien , Gaborit Pascal , Clervil Emmanuelle , Epelboin Yanouk , de Thoisy Benoit , Dusfour Isabelle , Duchemin Jean-Bernard , Lavergne Anne
Published: September 2024

Pathogens transmitted by mosquitoes (Diptera, Culicidae) in sylvatic or urban cycles involve wild or domestic animals and humans, driven by various mosquito species with distinct host preferences. Understanding mosquito–host associations is crucial for ecological insights and pathogen surveillance. In this study, we analyzed mosquito blood meals from coastal French Guiana by amplifying and sequencing host DNA from blood-fed females. Using the 12S ribosomal RNA gene and Sanger sequencing, we identified blood meals from 26 mosquito species across six genera, with 59% belonging to the Culex genus. Nanopore sequencing of selected samples showed 12 mosquito species with one to three mixed blood-meal sources. Mammals were the primary hosts (88%), followed by birds (7%), squamates (3%), and amphibians (2%), indicating a strong preference for mammalian hosts. A total of 46 vertebrate host species were identified, demonstrating high host diversity. This research provides insights into mosquito host usage and highlights the complexities of monitoring arboviruses of public health concern.
Respiratory and Neurodevelopmental Outcomes at 3 Years of Age of Neonates Diagnosed with Sleep-Disordered Breathing
Authors: Mehta Bhavesh , Waters Karen , Fitzgerald Dominic , Badawi Nadia
Published: September 2024

Objectives: Understanding the long-term consequences of sleep-disordered breathing (SDB) in neonates is crucial. A lack of consensus on diagnostic and treatment thresholds has resulted in limited research in this area. Our study aims to describe the trajectory of SDB in a cohort of high-risk neonates and their respiratory and neurodevelopmental outcomes at 3 years of age, and explore the relationship between SDB during early infancy and neurocognitive outcomes. Methods: A retrospectively identified cohort of neonates with moderate–severe SDB were prospectively followed at 3 years of age. Data collected included last polysomnography (PSG) parameters up to the age of 3 years and sleep physician’s recommendations, duration of CPAP use, compliance with treatment, timing of SDB resolution, and neurodevelopmental outcomes. Univariate and multivariate logistic regression analyses were performed to evaluate the association between important respiratory and sleep breathing parameters with the developmental outcomes. Results: Eighty neonates were included. Respiratory and developmental outcomes were available for 58 (72.5%) and 56 (70%) patients, respectively. In most patients (47/58, 81%), SDB had resolved by 3 years of age. Survival without major developmental delay was seen in 32/56 (57%), but a significant proportion (21/56, 37.5%) demonstrated global developmental delay. Following univariate analysis, primary diagnosis, apnoea–hypopnoea index (AHI) at the time of last PSG and SDB outcome was significantly associated with developmental delay. However, these associations were not seen in multivariate analysis. Conclusions: Despite severity at baseline, SDB resolved in the majority of patients with time and treatment. Although statistically insignificant, logistic regression analysis identified some clinically important associations between neonatal SDB and neurodevelopmental outcomes.
The DySLOH Study: Comparative Evaluation of the Results between the ProFlor and Lichtenstein Techniques for Open Inguinal Hernia Repair—A Randomized Controlled Trial
Authors: Romano Giorgio , Di Buono Giuseppe , Rodolico Vito , Romano Giorgio , Barletta Gabriele , Zanghì Guido , Calò Pietro Giorgio , Buscemi Salvatore , Agrusa Antonino
Published: September 2024

Background: The Lichtenstein open anterior approach with static flat meshes, the most popular inguinal hernia repair technique, has raised concerns regarding mesh fixation, defect patency, and poor quality biological response. To address these issues, the 3D dynamic ProFlor scaffold promoting a fixation-free hernia defect obliteration has been developed as an alternative. Methods: The results of open inguinal hernia repair with the ProFlor approach compared with those of the Lichtenstein repair were evaluated. Results: In a time frame of 24 months, two cohorts of patients were enrolled, 95 in the ProFlor group and 93 in the Lichtenstein group. ProFlor demonstrated superior outcomes compared to the Lichtenstein technique, with shorter procedure times, decreased intraoperative complications, and lower rates of postoperative complications. Additionally, ProFlor provided enhanced postoperative pain relief, a faster return to daily activities, and no long-term discomfort. No chronic pain was reported in the ProFlor group and 11.8% reported chronic pain in the Lichtenstein group. Conclusions: The results highlight the need to reevaluate the conventional Lichtenstein approach and align it with recent scientific progress. Further consideration of the evolving understanding of inguinal pathophysiology and groin protrusion genesis is crucial for advancing surgical techniques.
Endoscopic Submucosal Dissection for Early Gastric Cancer Exceeding Expanded Criteria—Long-Term Outcomes from the German ESD Registry
Authors: Riedl Kathrin , Probst Andreas , Ebigbo Alanna , Steinbrück Ingo , Allgaier Hans-Peter , Albers David , Mende Matthias , Anzinger Michael , Schirra Joerg , Rempel Viktor , Lorenz Albrecht , Faiss Siegbert , Wallstabe Ingo , Denzer Ulrike , Wannhoff Andreas , Dumoulin Franz Ludwig , Muzalyova Anna , Messmann Helmut
Published: September 2024

Background and aims: Endoscopic submucosal dissection (ESD) has become a standard treatment for early gastric cancer (EGC), often fulfilling guideline criteria (GC) or expanded criteria (EC). When lesions exceed the EC, surgical resection is recommended. However, a subgroup of these patients are not treated surgically. The aim of this study was to investigate the long-term follow-up of patients after ESD for EGC outside the EC (out of indication; OI). Methods: Patients who were included in the prospective German ESD registry were analyzed when ESD was performed for EGC. Patients were stratified in three groups according to histopathological features (GC, EC and OI). The results were evaluated in terms of patient characteristics, procedure characteristics and follow-up data. Results: Over a 48-month period, 195 patients from 14 German centers were included. In total, 71 lesions (36.4%) met the guideline criteria, 70 lesions (35.9%) corresponded to the expanded criteria and 54 lesions (27.7%) turned out to be OI. The R0 resection rate was significantly higher for the GC and EC groups than for the OI group (94.4% vs. 84.3% vs. 55.6%, p < 0.001). Additional surgery was not performed in 72% (39/54) of patients in the OI group. During a mean follow-up of 37 months, overall survival showed no significant difference between the EC and OI groups when endoscopic follow-up was performed without additional surgery (p = 0.064). Conclusions: The results show that a good long-term survival can be achieved after ESD for patients with OI lesions without additional surgery. The treatment decision has to be made on an individual basis, taking the patient’s comorbidities and the risk of surgical resection into account.
The Legacy of Harrington’s Rod and the Evolution of Long-Segment Constructs in Spine Surgery
Authors: Amadi Iheanyi J. , Kabangu Jean-Luc K. , Bhargav Adip G. , Camarata Paul J.
Published: September 2024

This paper delves into the historical evolution of spinal surgery, focusing on the pivotal role of the Harrington rod in treating spinal deformities. Introduced in 1955, the Harrington rod marked a significant breakthrough in neurosurgery, especially for scoliosis treatment, by offering a novel approach to spinal stabilization. Through a retrospective analysis, this study examines the development and impact of the Harrington rod, highlighting Dr. Paul Harrington’s contributions to spinal surgery. His innovative technique revolutionized the management of spinal deformities, laying the groundwork for future advancements in spinal instrumentation. Despite initial skepticism, Harrington’s methods gained acceptance, significantly influencing neurosurgical practices and patient outcomes. This study also explores subsequent advancements that built on Harrington’s work, including the transition to long-segment spine constructs and the introduction of segmental pedicle screws, which allowed for more precise deformity correction. Reflecting on Harrington’s legacy, this paper acknowledges the continuous evolution of spinal surgery, driven by the interplay between clinical challenges and technological innovations. Harrington’s pioneering spirit exemplifies the ongoing pursuit of better surgical outcomes, underscoring the importance of innovation in the field of neurosurgery.
The Evaluation of Immune Checkpoint Inhibitors and BRAF/MEK Inhibitors in Different Therapy Lines for Metastatic Melanoma: A Retrospective Study
Authors: Okuda-Hiwatashi Saki , Amagai Ryo , Fujimura Taku , Kambayashi Yumi , Watanabe-Takahashi Manami , Yamazaki Emi , Tamabuchi Erika , Itabashi Chisato , Hashimoto Akira , Asano Yoshihide
Published: September 2024

Background: Nivolumab plus ipilimumab (nivo/ipi) combination therapy is highly effective in treating advanced melanoma, but serious immune-related adverse events (irAEs) are prevalent. The overall response rate (ORR) of the BRAF inhibitor plus MEK inhibitor (BRAFi/MEKi) combination therapy for BRAFV600-mutant advanced melanoma surpasses that of immune checkpoint inhibitors (ICIs). However, the OS and PFS of BRAFi/MEKi combination therapy are inferior to those of ICIs. Methods: We retrospectively evaluated 22 melanoma patients treated with nivo/ipi therapy and 13 patients treated with encorafenib plus binimetinib (enco/bini) between November 2018 and July 2023. Results: The ORR of nivo/ipi for metastatic melanoma patients was significantly higher in the first-line cohort [60.0% (95% CI: 31.2–83.3%)] than in the second-line or beyond cohort [8.3% (95% CI: 0–37.5%)], whereas the ORR of enco/bini was comparable between the first-line cohort [75.0% (95% CI: 28.9–96.6%)] and the second-line or beyond cohort [77.8% (95% CI: 44.3–94.7%)]. The median PFS of nivo/ipi significantly improved in the first-line cohort [7.7 months (95% CI: 2.0–11.9)] compared to the second-line or beyond cohort [2.3 months (95% CI: 0.5–6.0)] (p = 0.0109). In addition to efficacy, the incidence of grade 3 or greater AEs was comparable in the first-line and second-line or beyond cohorts. Conclusions: Although our present data are based on a small number of cases, they suggest that nivo/ipi should be administered as the first-line therapy for the treatment of BRAFV600-mutant metastatic melanoma, rather than enco/bini, aligning with findings from previous clinical trials.
Overall Survival and Cancer-Specific Mortality in Patients with Prostate Cancer Undergoing Definitive Therapies: A Narrative Review
Authors: Kawase Makoto , Nakane Keita , Iinuma Koji , Kawase Kota , Taniguchi Tomoki , Tomioka Masayuki , Tobisawa Yuki , Koie Takuya
Published: September 2024

The overall survival (OS) of patients with prostate cancer (PCa) who receive locally definitive therapy is generally better than that of patients who do not receive definitive therapy. There is no difference in the incidence of local recurrence or distant metastasis between treatment modalities. Because the prognosis of PCa is relatively good, many studies have focused on quality of life after treatment as an endpoint. However, a limited number of patients develop biochemical recurrence after definitive treatment for PCa and subsequently develop distant metastasis or die from PCa. Therefore, we believe that preventing local recurrence and distant metastasis and prolonging the OS should be emphasized when selecting a treatment modality for PCa. In this review, the significance and usefulness of radical prostatectomy and radiation therapy as the main modalities of definitive therapies for local PCa and locally advanced PCa were evaluated, as well as the outcomes of OS and PCa-specific mortality and the treatment options after biochemical recurrence to improve the oncological outcomes.
Metabolic Side Effects of Risperidone in Pediatric Patients with Neurological Disorders: A Prospective Cohort Study
Authors: Alsabhan Jawza F. , Al Backer Nouf Backer , Hassan Fatimah M. , Albaker Awatif B. , Assiry Ghadeer
Published: September 2024

Background/Objectives: Risperidone-related metabolic side effects in children have been primarily linked to variations between guideline-recommended and clinical treatment procedures. We explored the metabolic effects of risperidone administration in pediatric patients diagnosed with neurological disorders, thus evaluating its influence on metabolic indicators. Methods: This prospective cohort study gathered data from electronic health records, medical databases, and clinical reports. These data included patient demographics (e.g., age, sex, and body mass index) and information on risperidone use, including dosage, dosing frequency, and treatment duration. Additionally, laboratory tests were conducted at baseline and during treatment, along with other pertinent clinical variables. Result: A total of 52 eligible children (male; 73.1%) with neurological disorders treated with risperidone were included. The mean age was 13.4 ± 2.2 years. Risperidone was administered to 32.7% of patients for <2 years, 40.4% for 2–5 years, and 26.9% for 6–9 years, with a mean duration of 3.6 years. Most (53.8%) of the children experienced at least one metabolic side effect, with hyperlipidemia being the most common (34.6%). The median prolactin level increased slightly from 448.5 ng/mL at baseline to 479 ng/mL after 6–8 weeks. No significant associations were found between age, sex, duration of treatment, dosage form, dosing frequency, and hemoglobin A1c levels. Conclusion: Monitoring metabolic and anthropometric parameters in children receiving risperidone for neurological disorders is cardinal. Clinicians should consider individualized treatment plans, closely monitor metabolic markers, and address potential risks associated with long-term risperidone use in this vulnerable population.
Condylar Remodeling and Skeletal Changes Following Occlusal Splint and Manual Therapy: A Cone Beam Computed Tomography Study in Temporomandibular Disorders
Authors: Tăut Manuela , Barbur Ioan , Hedeșiu Mihaela , Ban Alina , Leucuța Daniel , Negucioiu Marius , Buduru Smaranda Dana , Ilea Aranka
Published: September 2024

Background: Temporomandibular disorders (TMD) may be associated with degenerative disease of temporomandibular joint (TMJ), such as condyle erosion and subchondral cysts. Occlusal splint and cranio-mandibular manual therapy, or combined therapy, is recommended as a conservative treatment to alleviate pain-related signs and symptoms in TMD. This study aimed to assess osseous condylar changes and skeletal changes following occlusal splint and cranio-mandibular manual therapy in TMD using cone beam computed tomography (CBCT). Methods: A retrospective cohort study included 24 patients diagnosed with TMD. Combined therapy was performed until pain-related signs and symptoms disappeared. CBCT scans were performed before and after therapy. Osseous structure of condyles and their subsequent modifications were analyzed on CBCT images: flattening, erosion, and subchondral cyst. Sella-Nasion-A point (SNA), Sella-Nasion-B point (SNB), A point-Nasion-B point (ANB), Sella-Articulare-Gonion (Condylar angle), and anterior and posterior facial height (AFH, PFH) were measured on CBCT-generated lateral cephalograms. A paired t-test, Wilcoxon rank-sum test, McNemar test, and Stuart–Maxwell test were used for the statistical analyses. Results: The treatment period with combined therapy was 7.42 ± 3.27 months, and 21 out of 33 TMJ presenting degenerative disease (63.6%) had significant complete remodeling (p < 0.05). Following therapy, SNB significantly decreased from 75.61 ±3.47° to 74.82 ± 3.41° (p = 0.02), ANB significantly increased from 4.05° (3.35–4.9°) to 4.8° (3.3–6.12°) (p < 0.001), AFH significantly increased from 112.85 mm (109.28–118.72) to 115.3 mm (112.58–118.88) (p < 0.001), PFH/AFH significantly decreased from 64.17 (61.39–66.1) to 63 (59.68–64.51) (p = 0.012), and condylar angle significantly increased from 140.84 ± 8.18° to 144.42 ± 8.87° (p = 0.007). Conclusion: Combined therapy promoted significant condylar remodeling in TMJ degenerative disease, along with skeletal changes (mandibular retrusion and increase in facial height). Therapeutic strategies should consider condylar remodeling in TMD. Skeletal and dental parameters should be evaluated prior to occlusal splint therapy.
Assessment of the In Vitro Phosphatidylinositol Glycan Class A (PIG-A) Gene Mutation Assay Using Human TK6 and Mouse Hepa1c1c7 Cell Lines
Authors: Zhang Wenhao , Miller Charles A. , Wilson Mark J.
Published: September 2024

Gene mutations linked to diseases like cancer may be caused by exposure to environmental chemicals. The X-linked phosphatidylinositol glycan class A (PIG-A) gene, required for glycosylphosphatidylinositol (GPI) anchor biosynthesis, is a key target locus for in vitro genetic toxicity assays. Various organisms and cell lines may respond differently to genotoxic agents. Here, we compared the mutagenic potential of directly genotoxic ethyl methane sulfonate (EMS) to metabolically activated pro-mutagenic polycyclic aromatic hydrocarbons (PAHs). The two classes of mutagens were compared in an in vitro PIG-A gene mutation test using the metabolically active murine hepatoma Hepa1c1c7 cell line and the human TK6 cell line, which has limited metabolic capability. Determination of cell viability is required for quantifying mutagenicity. Two common cell viability tests, the MTT assay and propidium iodide (PI) staining measured by flow cytometry, were evaluated. The MTT assay overestimated cell viability in adherent cells at high benzo[a]pyrene (B[a]P) exposure concentrations, so PI-based cytotoxicity was used in calculations. The spontaneous mutation rates for TK6 and Hepa1c1c7 cells were 1.87 and 1.57 per million cells per cell cycle, respectively. TK6 cells exposed to 600 µM and 800 µM EMS showed significantly higher mutation frequencies (36 and 47 per million cells per cell cycle, respectively). Exposure to the pro-mutagen benzo[a]pyrene (B[a]P, 10 µM) did not increase mutation frequency in TK6 cells. In Hepa1c1c7 cells, mutation frequencies varied across exposure groups (50, 50, 29, and 81 per million cells per cell cycle when exposed to 10 µM B[a]P, 5-methylcholanthrene (5-MC), chrysene, or 16,000 µM EMS, respectively). We demonstrate that the choice of cytotoxicity assay and cell line can determine the outcome of the Pig-A mutagenesis assay when assessing a specific mutagen.
Risk Factors for Ovarian Cancer in South America: A Literature Review
Authors: Jara-Rosales Sergio , González-Stegmaier Roxana , Rotarou Elena S. , Villarroel-Espíndola Franz
Published: September 2024

Background/Objectives: In 2020, ovarian cancer ranked fourth in global incidence among gynecological cancers and remains the deadliest cancer affecting women’s health. Survival rates are significantly higher when the disease is detected at early stages; however, the lack of effective early detection methods underscores the importance of identifying risk factors in order to implement preventive strategies. The objective of this work is to provide an overview of the risk factors of ovarian cancer in South America, emphasizing those linked to social determinants, genetic components, and comorbidities. Methods: A literature search was performed using PubMed and Google Scholar. MeSH descriptors and keywords, such as “BRCA1 genes,” “BRCA2 genes”, “Latin America”, and “ovarian neoplasms” were used, along with terms related to socioeconomic and health factors. Inclusion criteria focused on original studies published in the last five years involving South American women. Results: Studies were identified from Argentina, Brazil, Chile, Colombia, Ecuador, and Peru. These studies addressed genetic factors, health status at diagnosis, and sociodemographic factors, revealing important data gaps, particularly on contraception and hormone replacement therapy. The prevalence of BRCA1 and BRCA2 mutations in South America is estimated to be 15–20% among women with inherited risk factors. Social, demographic and economic factors vary by country, although commonalities include a higher prevalence among women over 50 years of age, those with limited education, and those who face barriers to accessing health care. Conclusions: Although the literature does not conclusively establish a direct link between obesity and/or diabetes and the development of ovarian cancer, the indirect association highlights the need for further clinical studies. A general research gap related to risk factors of ovarian cancer could be observed in the South American region.
Retinal Microvascular Changes in Association with Endothelial Glycocalyx Damage and Arterial Stiffness in Patients with Diabetes Mellitus Type 2: A Cross-Sectional Study in a Greek Population
Authors: Agapitou Chrysa , Sergentanis Theodoros N. , Thymis John , Pavlidis George , Lampsas Stamatios , Korakas Emmanouil , Kountouri Aikaterini , Pliouta Loukia , Karmiris Efthymios , Lagiou Areti , Theodossiadis Panagiotis , Lambadiari Vaia , Ikonomidis Ignatios , Chatziralli Irini
Published: September 2024

Purpose: To evaluate the potential association between endothelial glycocalyx damage, as well as arterial stiffness, and the retinal changes on optical coherence tomography (OCT) and OCT-angiography (OCT-A) in patients with type 2 diabetes mellitus (DM). Methods: Participants in this cross-sectional study were 65 patients with DM type 2 and 42 age- and gender-matched controls without DM. The demographic and clinical characteristics of the participants were recorded. All patients underwent a thorough ophthalmological examination and multimodal imaging, including fundus photography, OCT, and OCT-A. In addition, evaluation of the endothelial glycocalyx thickness by measuring the perfused boundary region (PBR5-25) of the sublingual microvessel, as well as of the arterial stiffness, by measuring the carotid–femoral pulse wave velocity (PWV), the central aortic pressures and the augmentation index (Aix) was performed. Univariate and multivariate logistic regression analysis was performed for the examination of the potential association between the eye imaging variables and the cardiovascular-related variables. The odds ratios (OR) with the respective 95% confidence intervals (CI) were calculated. A p-value < 0.05 was considered statistically significant. Results: Patients with DM presented significantly higher PBR5-25 compared to controls without DM (p = 0.023). At the univariate analysis, increased PBR5-25 (≥2.19 μm vs. <2.19 μm) was associated with decreased peripapillary VD at the superior quadrant (univariate OR (95% CI) = 0.34 (0.12–0.93), p = 0.037). Multivariate logistic regression analysis showed that increased PWV (≥13.7 m/s vs. <13.7 m/s) was associated with an increased foveal avascular zone (FAZ) area on OCT-A (p = 0.044) and increased FAZ perimeter (p = 0.048). Moreover, increased Aix (≥14.745% vs. <14.745%) was associated with diabetic macular edema (DME) presence (p = 0.050) and increased perifoveal and parafoveal superior and temporal thickness on OCT (p < 0.05 for all associations). Conclusions: Markers of endothelial damage and arterial stiffness were associated with structural and microvascular retinal alterations in patients with DM, pointing out that OCT-A could be a useful biomarker for detecting potential cardiovascular risk in such patients.
The Variation in Boulder Bars Triggered by the 2018 Sedongpu Natural Dam Failure in the Yarlung Tsangpo River
Authors: Jiang Xiangang , Xie Xinlin , Guo Zhehao , Wörman Anders , Liu Xingrong , Liu Weiming , Xie Yiqin
Published: September 2024

Natural dams are formed most often in narrow, steep valleys in high mountains. The outburst floods triggered by natural dam failures result in the topography and landforms successively being altered. Boulder bars are common natural structures that are selected here to quantitatively evaluate the impact of outburst floods on the topographical and landform variations in downstream channels. In this study, we selected the Sedongpu natural dam on the Yarlung Tsangpo River formed as a result of a landslide in 2018 as an example, and studied the geomorphological changes in a river reach located 173 km downstream of the Sedongpu natural dam. The sizes and shapes of the boulder bars in this area were statistically analyzed. The results show that there are three shape types of boulder bars in this area, i.e., sickle, bamboo leaf and oval. Furthermore, it found that the relationship between the lengths and widths of boulder bars is similar before and after outburst floods, as is the relationship between perimeters and lengths of boulder bars, which means these relationships are not affected by outburst floods. And the perimeters of boulder bars are almost twice their lengths. In addition, the relationship between the areas and lengths of boulder bars follows a power function. The most important finding is that the riverine morphological features conserved self-similarity due the influence of the outburst flood erosion triggered by a natural dam failure. This finding adds to the previous observations since dam failures introduce sudden and dominating impacts on river systems.
Design and Performance of a Novel Tapered Wing Tiltrotor UAV for Hover and Cruise Missions
Authors: Rojo-Rodriguez Edgar Ulises , Rojo-Rodriguez Erik Gilberto , Araujo-Estrada Sergio A. , Garcia-Salazar Octavio
Published: September 2024

This research focuses on a novel convertible unmanned aerial vehicle (CUAV) featuring four rotors with tilting capabilities combined with a tapered form. This paper studies the transition motion between multirotor and fixed-wing modes based on the mechanical and aerodynamics design as well as the control strategy. The proposed CUAV involves information about design, manufacturing, operation, modeling, control strategy, and real-time experiments. The CUAV design considers a fixed-wing with tiltrotors and provides the maneuverability to perform take-off, hover flight, cruise flight, and landing, having the characteristics of a helicopter in hover flight and an aircraft in horizontal flight. The manufacturing is based on additive manufacturing, which facilitates the creation of a lattice structure within the wing. The modeling is obtained using the Newton–Euler equations, and the control strategy is a PID controller based on a geometric approach on SE(3). Finally, the real-time experiments validate the proposed design for the complete regime of flight, and the research meticulously evaluates the feasibility of the prototype and its potential to significantly enhance the mission versatility.
The Tightening and Untightening Modeling and Simulation of Bolted Joints
Authors: Rousseau Rashique Iftekhar , Bouzid Abdel-Hakim
Published: September 2024

Although bolted joints may appear simple and are easy to manipulate, they are challenging to model and analyze due to their complex structural patterns and statically indeterminate nature. Ensuring the structural integrity of these joints requires maintaining proper bolt preload and clamping force, which is crucial for preventing failures such as overload, excessive bearing stress, fatigue, and stripping caused by seizing or galling. Achieving the necessary clamping force involves carefully controlling the input tightening torque, which is divided into the pitch torque and the friction torques at the bolt or nut bearing surfaces and in the engaged threads. The resulting clamping force is critical for generating the required force within the bolt. However, the achieved bolt force depends on several factors, such as friction at the joint’s contact surfaces, grip length, and the relative rotation between the bolt and nut during tightening. Friction at the contact surfaces, particularly beneath the bolt head or nut and between the threads, consumes a significant portion of the applied tightening torque—approximately 90%. This paper explores the three existing bolt internal pitch, bearing, and thread friction torques that are generated by the external applied torque in a bolted joint, as well as their contributions and variations throughout a loading cycle composed of three phases: tightening, settling, and untightening. An analytical model is developed to determine these torque components, and its results are compared with those obtained from finite element (FE) modeling and experimental testing from previous studies. Finally, this study examines the torque–tension relationship during bolt tightening, offering insights into the required accuracy of bolt and clamped member stiffness. The bolt samples used in this study include M12 × 1.75 and M36 × 4 hex bolts.
Design and Analysis of a Highly Reliable Permanent Magnet Synchronous Machine for Flywheel Energy Storage
Authors: Jiang Xinjian , Zhang Lei , Li Fuwang , Zhao Zhenghui
Published: September 2024

This article aims to propose a highly reliable permanent magnet synchronous machine (PMSM) for flywheel energy-storage systems. Flywheel energy-storage systems are large-capacity energy storage technologies suitable for the short-term storage of electrical energy. PMSMs have been used in the flywheel energy-storage systems due to their advantages. One of the key requirements for PMSMs in flywheel energy-storage systems is high reliability. A double redundant winding structure is adopted to ensure fault-tolerant operation of the PMSM. The stator is designed with auxiliary teeth to reduce the short-circuit current. Moreover, the number of slots and poles is determined to ensure the winding factor, heat dissipation, and reduce losses. Moreover, the dual three-phase stator winding structure and auxiliary teeth are adopted on the PMSM to improve reliability. Afterward, the electromagnetic performance is analyzed, and the mechanical stress is investigated to ensure mechanical strength. Finally, a prototype is built and tested to verify the theoretical analysis and performance of the PMSM.
Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model
Authors: Rodríguez-Expósito Rubén L. , Sifaoui Ines , Salazar-Villatoro Lizbeth , Bethencourt-Estrella Carlos J. , Fernández José J. , Díaz-Marrero Ana R. , Sutak Robert , Omaña-Molina Maritza , Piñero José E. , Lorenzo-Morales Jacob
Published: September 2024

Acanthamoeba is a ubiquitous genus of amoebae that can trigger a severe and progressive ocular disease known as Acanthamoeba Keratitis (AK). Furthermore, current treatment protocols are based on the combination of different compounds that are not fully effective. Therefore, an urgent need to find new compounds to treat Acanthamoeba infections is clear. In the present study, we evaluated staurosporine as a potential treatment for Acanthamoeba keratitis using mouse cornea as an ex vivo model, and a comparative proteomic analysis was conducted to elucidate a mechanism of action. The obtained results indicate that staurosporine altered the conformation of actin and tubulin in treated trophozoites of A. castellanii. In addition, proteomic analysis of treated trophozoites revealed that this molecule induced overexpression and a downregulation of proteins related to key functions for Acanthamoeba infection pathways. Additionally, the ex vivo assay used validated this model for the study of the pathogenesis and therapies of AK. Finally, staurosporine eliminated the entire amoebic population and prevented the adhesion and infection of amoebae to the epithelium of treated mouse corneas.
Presence and Causes of Sterilization Equipment Failures with Biological Indicators in Dental Offices in Mexico: A Longitudinal Cohort
Authors: Patiño-Marín Nuria , Villa-García Lorena Dafnee , Terán-Figueroa Yolanda , Medina-Solis Carlo Eduardo , Rangel-Flores Yesica Yolanda , Salas-Orozco Marco Felipe , Martínez-Gutiérrez Fidel , Aguirre-López Eva Concepción
Published: September 2024

Background/Objectives: Sterilization in dental practice is crucial for infection prevention. The aim of this study was to identify the presence and causes of bacterial growth using biological indicators in dental sterilization equipment in San Luis Potosí, S.L.P., Mexico, with different consecutive measurements over a year. Methods: This longitudinal cohort, conducted from January 2022 to January 2024 in San Luis Potosí, Mexico, aimed to identify the presence and causes of bacterial growth in dental sterilization equipment using biological indicators. A total of 207 dental offices were approached, and 175 participated, providing data through questionnaires and monitoring sterilization cycles with BIs. The checks were bimonthly for one year, with a total of six checks. Results: (a) An 11% (n = 1188) incidence of bacterial growth was observed, with a higher percentage in dry heat equipment (13%). (b) Upon analyzing the six consecutive verifications over a year, no statistically significant differences were observed in the failures of the sterilization cycles when comparing the two pieces of equipment. (c) Error in temperature and time of the equipment (OR = 4.0, 95% CI = 1.6–3.9, p = 0.0001) was significantly associated with the presence of bacterial growth during the one-year period. Conclusions: Monitoring sterilization cycles and identifying the causes of bacterial growth with different consecutive verifications decreased the presence of bacterial growth (failures) during the one-year period.
Mortality Risk and Antibiotic Therapy for Patients with Infections Caused by Elizabethkingia Species—A Meta-Analysis
Authors: Huang Chienhsiu , Kuo Sufang , Lin Lichen
Published: September 2024

Background and Objectives: Patients with infections caused by Elizabethkingia species require prompt identification and effective antibiotic treatment since these spp. are typically resistant to multiple antibiotics and variable susceptibility patterns. Understanding the mortality risk of this disease is difficult because of the relatively low incidence of infections caused by Elizabethkingia spp. and the lack of published systematic evaluations of the risk factors for mortality. The aim of the present study was to investigate risk factors for mortality in patients with infections caused by Elizabethkingia spp. by conducting a meta-analysis of existing studies on these infections. Materials and Methods: Studies comparing patients who died from infections caused by Elizabethkingia spp. with patients who survived were considered for inclusion. Studies that reported one or more risk factors for mortality were considered. Clinical predisposing variables, predisposing comorbidities, and clinical outcomes of antibiotic treatment were among the risk factors for mortality. Results: The meta-analysis included twenty studies with 990 patients, and 298 patients (30.1%) died. The following risk factors for mortality were identified: intensive care unit admission, the need for mechanical ventilation, immunosuppressive or steroid therapy use, pneumonia, comorbid liver disease, and the use of inappropriate antimicrobial therapy. Conclusions: The use of appropriate antimicrobial therapy is critical for the effective management of infections caused by Elizabethkingia spp. Antimicrobial susceptibility testing would be a more reliable means of guiding treatment. The identification of the best antimicrobial drugs is needed to ensure optimal treatment recommendations for treating Elizabethkingia-related infections.
Effect of Surface Treatment on Tensile Strength of Steel Single Lap Joints Bonded with Double-Sided Acrylic Foam Tapes for Naval Applications
Authors: Di Bella Guido , Chairi Mohamed , Denaro Antonio , Bado Adriano
Published: September 2024

This study investigated single lap joints in steel used for naval carpentry. The surface was mechanically treated, and then a double-sided acrylic foam tape was applied with varying surface preparation conditions. Specifically, three different conditions were examined. Tensile tests revealed that changing the type of surface preparation significantly affects the mechanical strength of the joints. The best mechanical properties were achieved when a primer was used. Our results demonstrate that this method can be effectively employed in naval applications as an alternative to welding for non-structural applications, such as the installation of brackets for mounting electrical devices (e.g., sockets).
Exploring the Metabolism of Flubrotizolam, a Potent Thieno-Triazolo Diazepine, Using Human Hepatocytes and High-Resolution Mass Spectrometry
Authors: Gameli Prince Sellase , Kutzler Johannes , Berardinelli Diletta , Carlier Jeremy , Auwärter Volker , Busardò Francesco Paolo
Published: September 2024

Background: The abuse of psychoactive substances presents challenges in clinical and forensic toxicology. The emergence of novel and potent drugs that pose significant health risks, in particular towards frequent abusers and users unaware of the ingredients, further complicates the situation. Designer benzodiazepines have become a fast-growing subgroup of these new psychoactive substances (NPSs), and their overdose may potentially turn fatal, especially when combined with other central nervous system depressants. In 2021, flubrotizolam, a potent thieno-triazolo designer benzodiazepine, emerged on the illicit market, available online as a “research chemical”. The identification of markers of consumption for this designer benzodiazepine is essential in analytical toxicology, especially in clinical and forensic cases. Methods: We therefore aimed to identify biomarkers of flubrotizolam uptake in ten-donor-pooled human hepatocytes, applying liquid chromatography high-resolution mass spectrometry and software-aided data mining supported by in silico prediction tools. Results: Prediction studies resulted in 10 and 13 first- and second-generation metabolites, respectively, mainly transformed through hydroxylation and sulfation, methylation, and glucuronidation reactions. We identified six metabolites after 3 h human hepatocyte incubation: two hydroxylated metabolites (α- and 6-hydroxy-flubrotizolam), two 6-hydroxy-glucuronides, a reduced-hydroxy-N-glucuronide, and an N-glucuronide. Conclusions: We suggest detecting flubrotizolam and its hydroxylated metabolites as markers of consumption after the glucuronide hydrolysis of biological samples. The results are consistent with the in vivo metabolism of brotizolam, a medically used benzodiazepine and a chloro-phenyl analog of flubrotizolam.