bluuebunny/crossref_metadata_2025 huggingface dataset:
A Fast Computer-Generated Holographic Method for VR and AR Near-Eye 3D Display
Authors: Yang Xin , Zhang HongBo , Wang Qiong-Hua
Published: October 2019

A fast computer-generated holographic method with multiple projection images for a near-eye VR (Virtual Reality) and AR (Augmented Reality) 3D display is proposed. A 3D object located near the holographic plane is projected onto a projection plane to obtain a plurality of projected images with different angles. The hologram is calculated by superposition of projected images convolution with corresponding point spread functions (PSF). Holographic 3D display systems with LED as illumination, 4f optical filtering system and lens as eyepiece for near-eye VR display and holographic optical element (HOE) as combiner for near-eye AR display are designed and developed. The results show that the proposed calculation method is about 38 times faster than the conventional point cloud method and the display system is compact and flexible enough to produce speckle noise-free high-quality VR and AR 3D images with efficient focus and defocus capabilities.
Evaluating the Effect of Wheel Polygons on Dynamic Track Performance in High-Speed Railway Systems Using Co-Simulation Analysis
Authors: Song Ying , Du Yanliang , Zhang Xuemei , Sun Baochen
Published: October 2019

With increases in train speed and traffic density, problems due to wheel polygons and those caused by wheel–rail impacts will increase accordingly, which will affect train operational safety and passenger ride comfort. This paper investigates the effects of polygonal wheels on the dynamic performance of the track in a high-speed railway system. The wheel–rail interaction forces caused by wheel polygons are determined using a dynamic vehicle–track model, and the results are entered into a slab track finite element model. The influence of the harmonic order and out-of-roundness (OOR) amplitude of wheel polygons on the transient dynamic characteristics of the track(von Mises equivalent stress, displacement, and acceleration) is examined under high-speed conditions. The results indicate that the vibration acceleration and von Mises equivalent stress of the rail increase in proportion to the harmonic order and the OOR amplitude and velocity of a polygonized wheel. The vibration displacement of the rail first increases and then decreases with a change in the harmonic order, and reaches a maximum at the ninth order. The dynamic responses of the concrete slab layer, cement-asphalt layer, and support layer increase linearly with the harmonic order and amplitude of wheel polygons and decrease from top to bottom. Through a combination of numerical simulations and real-time monitoring of rail vibrations, this study provides guidance on potential sensor locations to identify polygonized wheels before they fail.
Deep-Learning-Based Defective Bean Inspection with GAN-Structured Automated Labeled Data Augmentation in Coffee Industry
Authors: Chou Yung-Chien , Kuo Cheng-Ju , Chen Tzu-Ting , Horng Gwo-Jiun , Pai Mao-Yuan , Wu Mu-En , Lin Yu-Chuan , Hung Min-Hsiung , Su Wei-Tsung , Chen Yi-Chung , Wang Ding-Chau , Chen Chao-Chun
Published: October 2019

In the production process from green beans to coffee bean packages, the defective bean removal (or in short, defect removal) is one of most labor-consuming stages, and many companies investigate the automation of this stage for minimizing human efforts. In this paper, we propose a deep-learning-based defective bean inspection scheme (DL-DBIS), together with a GAN (generative-adversarial network)-structured automated labeled data augmentation method (GALDAM) for enhancing the proposed scheme, so that the automation degree of bean removal with robotic arms can be further improved for coffee industries. The proposed scheme is aimed at providing an effective model to a deep-learning-based object detection module for accurately identifying defects among dense beans. The proposed GALDAM can be used to greatly reduce labor costs, since the data labeling is the most labor-intensive work in this sort of solutions. Our proposed scheme brings two main impacts to intelligent agriculture. First, our proposed scheme is can be easily adopted by industries as human effort in labeling coffee beans are minimized. The users can easily customize their own defective bean model without spending a great amount of time on labeling small and dense objects. Second, our scheme can inspect all classes of defective beans categorized by the SCAA (Specialty Coffee Association of America) at the same time and can be easily extended if more classes of defective beans are added. These two advantages increase the degree of automation in the coffee industry. The prototype of the proposed scheme was developed for studying integrated tests. Testing results of a case study reveal that the proposed scheme can efficiently and effectively generate models for identifying defective beans with accuracy and precision values up to 80 % .
Theoretical Study on Geometries of Electrodes in Laboratory Electrical Resistivity Measurement
Authors: Hong Chang-Ho , Chong Song-Hun , Cho Gye-Chun
Published: October 2019

Electrical resistivity tests have been widely conducted in multiple scales, from a few centimeters to kilometers. While electrode spacing is used to define field resistance, laboratory measurements in a limited space need to consider electrode geometry. However, there are no studies that theoretically explore the effects of the geometries of electrodes and container size on laboratory electrical resistivity measurements. This study formulates a theoretical electrical resistance for the geometry of cylindrical electrodes and the size of a non-conductive container with the method of image charges. As a complementary study, experimental tests were conducted to verify the derived equations. The discussion includes the concepts of the spherical equivalent electrodes and a simple design method for container size.
Toolpath Strategies for 5DOF and 6DOF Extrusion-Based Additive Manufacturing
Authors: Jensen Mathias Laustsen , Mahshid Rasoul , D’Angelo Greta , Walther Jeppe U. , Kiewning Malte K. , Spangenberg Jon , Hansen Hans Nørgaard , Pedersen David Bue
Published: October 2019

This paper introduces two new deposition-strategies for five degrees of freedom (5DOF) and 6DOF extrusion-based additive manufacturing (AM), called the tool path projection- and parent-child-approach, respectively. The tool path projection method can be automated, and allows for the generation of concentric shells layers, which remedy geometrical deviations (known as the stair-case effect) that are typically seen in 3DOF AM processes that potentially require secondary post treatment by machining or grinding of the final part. In the parent-child approach, the designer specifies the manufacturing direction for each distinct feature, thereby helping to remove the need for support material, as well as enabling new features to be dynamically added to the part.
Topographic Effects on Titan’s Dune-Forming Winds
Authors: Larson Erik J. L.
Published: October 2019

The Cassini mission made an unexpected discovery when it found evidence of linear dune fields on Titan’s surface. The orientation of the dunes and their interaction with topography allow scientists to estimate the dominant wind direction on the surface of Titan. There is some consensus in the community that the dune-forming winds must be net westerly, however, there is an active debate about the dune-forming wind regime. This debate has been guided by several studies of Earth dune fields considered analogous to the Titan dunes including those in Namibia, the Sahara, the Serengeti, and China. Complicating this active debate about the surface wind regime is the fact that global circulation models (GCMs) have historically not been able to reproduce westerly surface winds in the tropics. Here we use the Titan Community Atmosphere Model (CAM) to quantify the impact of topography and an added torque on Titan’s dune-forming winds. Dunes tend to form at higher elevations on Titan, and adding topography to the model alters the near-surface wind directions, making them more westerly and consistent with the dune orientations. The addition of topography and added torque create a wind regime that is consistent with linear dunes in areas of stabilized sediment.
Discrimination of Oviposition Deterrent Volatile β-Ionone by Odorant-Binding Proteins 1 and 4 in the Whitefly Bemisia tabaci
Authors: Li Fengqi , Li Du , Dewer Youssef , Qu Cheng , Yang Zhen , Tian Jiahui , Luo Chen
Published: October 2019

: The whitefly, Bemisia tabaci, is an important invasive economic pest of agricultural crops worldwide. β-ionone has a significant oviposition repellent effect against B. tabaci, but the olfactory molecular mechanism of this insect for recognizing β-ionone is unclear. To clarify the binding properties of odorant-binding proteins (OBPs) with β-ionone, we performed gene cloning, evolution analysis, bacterial expression, fluorescence competitive binding assay, and molecular docking to study the binding function of OBP1 and OBP4 on β-ionone. The results showed that after the OBP1 and OBP4 proteins were recombined, the compound β-ionone exhibited a reduction in the fluorescence binding affinity to <50%, with a dissociation constant of 5.15 and 3.62 μM for OBP1 and OBP4, respectively. Our data indicate that β-ionone has high affinity for OBP1 and OBP4, which play a crucial role in the identification of oviposition sites in B. tabaci. The findings of this study suggest that whiteflies employ β-ionone compound in the selection of the suitable egg-laying sites on host plants during the oviposition behavior.
Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
Authors: Maity Nitu , Mishra Saroj
Published: October 2019

Human serum albumin (HSA), sourced from human serum, has been an important therapeutic protein for several decades. Pichia pastoris is strongly considered as an expression platform, but proteolytic degradation of recombinant HSA in the culture filtrate remains a major bottleneck for use of this system. In this study, we have reported the development of a medium that minimized proteolytic degradation across different copy number constructs. A synthetic codon-optimized copy of HSA was cloned downstream of α–factor secretory signal sequence and expressed in P. pastoris under the control of Alcohol oxidase 1 promoter. A two-copy expression cassette was also prepared. Culture conditions and medium components were identified and optimized using statistical tools to develop a medium that supported stable production of HSA. Comparative analysis of transcriptome data obtained by cultivation on optimized and unoptimized medium indicated upregulation of genes involved in methanol metabolism, alternate nitrogen assimilation, and DNA transcription, whereas enzymes of translation and secretion were downregulated. Several new genes were identified that could serve as possible targets for strain engineering of this yeast.
Proteases Immobilization for In Situ Time-Limited Proteolysis on MALDI Chips
Authors: Rosulek Michal , Darebna Petra , Pompach Petr , Slavata Lukas , Novak Petr
Published: October 2019

A large number of different enzyme immobilization techniques are used in the field of life sciences, clinical diagnostics, or biotechnology. Most of them are based on a chemically mediated formation of covalent bond between an enzyme and support material. The covalent bond formation is usually associated with changes of the enzymes’ three-dimensional structure that can lead to reduction of enzyme activity. The present work demonstrates a potential of an ambient ion-landing technique to effectively immobilize enzymes on conductive supports for direct matrix-assisted laser desorption/ionization (MALDI) mass spectrometry analyses of reaction products. Ambient ion landing is an electrospray-based technique allowing strong and stable noncovalent and nondestructive enzyme deposition onto conductive supports. Three serine proteolytic enzymes including trypsin, α-chymotrypsin, and subtilisin A were immobilized onto conductive indium tin oxide glass slides compatible with MALDI mass spectrometry. The functionalized MALDI chips were used for in situ time-limited proteolysis of proteins and protein–ligand complexes to monitor their structural changes under different conditions. The data from limited proteolysis using MALDI chips fits to known or predicted protein structures. The results show that functionalized MALDI chips are sensitive, robust, and fast and might be automated for general use in the field of structural biology.
Formation of a Pd/MgO Structured Catalyst for the Aqueous Oxidation of Silane to Silanol
Authors: Zhou Jun , Cao Xiaoqing , Wang Hongna , Li Song
Published: October 2019

The catalytic oxidation of silanes to produce silanols using water as an oxidant at mild temperatures is a major challenge in Si-H activation. Highly efficient and easy-to-recycle catalysts based on Pd nanoparticles are in high demand. In this study, Pd nanoparticles embedded in an MgO porous overlayer on an Mg plate as a structured catalyst was prepared by the plasma electrolyte oxidation (PEO) technique. The Pd/MgO catalyst is strongly anchored to the MgO plate, building a structured catalyst. Fabrication parameters such as the temperature of the electrolyte and applied voltage significantly influenced the structure of the obtained Pd/MgO catalyst and in turn its catalytic activity. The catalytic activities of Pd/MgO were evaluated by activation of a Si-H bond for catalyzing the aqueous oxidation of silanes to silanol at mild temperatures. The catalytic activity of Pd nanoparticles is favored by their electro-deficient state due to influence from the MgO substrate. The Pd/MgO catalyst exhibits good performance stability during recycling. This work paves the way for fabricating structured catalysts with long-term stability and enhanced metal–oxide interaction.
Update on the Role of the Non-Canonical Wnt/Planar Cell Polarity Pathway in Neural Tube Defects
Authors: Wang Mingqin , de Marco Patrizia , Capra Valeria , Kibar Zoha
Published: October 2019

Neural tube defects (NTDs), including spina bifida and anencephaly, represent the most severe and common malformations of the central nervous system affecting 0.7–3 per 1000 live births. They result from the failure of neural tube closure during the first few weeks of pregnancy. They have a complex etiology that implicate a large number of genetic and environmental factors that remain largely undetermined. Extensive studies in vertebrate models have strongly implicated the non-canonical Wnt/planar cell polarity (PCP) signaling pathway in the pathogenesis of NTDs. The defects in this pathway lead to a defective convergent extension that is a major morphogenetic process essential for neural tube elongation and subsequent closure. A large number of genetic studies in human NTDs have demonstrated an important role of PCP signaling in their etiology. However, the relative contribution of this pathway to this complex etiology awaits a better picture of the complete genetic architecture of these defects. The emergence of new genome technologies and bioinformatics pipelines, complemented with the powerful tool of animal models for variant interpretation as well as significant collaborative efforts, will help to dissect the complex genetics of NTDs. The ultimate goal is to develop better preventive and counseling strategies for families affected by these devastating conditions.
Heat Integration of a Boiler and Its Corresponding Environmental Study in an Oleochemical Production Plant: An Industry Case Study in Malaysia
Authors: Koh Kai Seng , Chew Shee Jia , Choo Chee Ming , Chok Vui Soon
Published: October 2019

The growing demands for oleochemical products are expected to reach approximately RM 157.59 billion by 2026 due to an increased drive from the food and beverages, chemicals, and pharmaceutical industries. However, this will lead to an increase in energy consumption and subsequent flue gas emission. Proper utilization of waste gas recovery systems is thus a major research area, focusing on reducing fuel consumption and emissions of greenhouse gases without affecting process performance. In this paper, a palm oil-based oleochemical plant is studied. The fuel consumption and emission of flue gas from a thermal oil boiler were measured and the feasibility of implementation of a waste heat recovery system and its environmental impact study. The results show that the implementation of such a system can reduce natural fuel gas consumption by 17.29% and approximately 149.29 t per annum of carbon dioxide gas (CO2). Moreover, the concentration of CO2 released into highly-populated communities is estimated through a Gaussian Plume Model at different wind speed conditions. The preliminary results show that the CO2 concentration at two locations—an apartment and a local school located within 1.5 km of the plant—is well below the concentration limit of 1.938 g/m3 recommended by the Wisconsin Department of Health and Services.
Synthesis of MgFe Layered Double Hydroxide from Iron-Containing Acidic Residual Solution and Its Adsorption Performance
Authors: Golban Alin , Lupa Lavinia , Cocheci Laura , Pode Rodica
Published: October 2019

The paper presents a new method of layered double hydroxide (LDH) synthesis starting from secondary sources, namely acidic residual solutions. The iron content of the acidic solution resulting from the pickling step of the hot-dip galvanizing process make it suitable to be used as an iron precursor in LDH synthesis. Here, Mg4Fe–LDH synthesized through the newly proposed method presented structural and morphological characteristics similar to the properties of layered double hydroxides synthesized from analytical-grade reagents. Moreover, the as-synthesized LDH and its calcined product presented efficient adsorption properties in the removal process of Mo(VI) from aqueous solutions. The adsorption studies are discussed from the equilibrium, kinetic, and thermodynamic points of view. The proposed novel technologies present both economic and environmental protection benefits.
Egestion Versus Excretion: A Meta-Analysis Examining Nutrient Release Rates and Ratios across Freshwater Fauna
Authors: Halvorson Halvor M. , Atkinson Carla L.
Published: October 2019

In aquatic settings, animals directly affect ecosystem functions through excretion of dissolved nutrients. However, the comparative role of egestion as an animal-mediated nutrient flux remains understudied. We conducted a literature survey and meta-analysis to directly compare nitrogen (N), phosphorus (P), and N:P of egestion compared to excretion rates and ratios across freshwater animals. Synthesizing 215 datasets across 47 animal species (all primary consumers or omnivores), we show that the total N and P egestion rates exceed inorganic N and P excretion rates but not total N and P excretion rates, and that proportions of P egested compared to excreted depend on body size and animal phylum. We further show that variance of egestion rates is often greater than excretion rates, reflecting greater inter-individual and temporal variation of egestion as a nutrient flux in comparison to excretion. At phylogenetic levels, our analysis suggests that Mollusca exhibit the greatest rates and variance of P egestion relative to excretion, especially compared to Arthropoda. Given quantitative evidence of egestion as a dominant and dynamic animal-mediated nutrient flux, our synthesis demonstrates the need for additional studies of rates, stoichiometry, and roles of animal egestion in aquatic settings.
A Comparison of Filtering Approaches Using Low-Speed DACs for Hardware-in-the-Loop Implemented in FPGAs
Authors: Yushkova Marina , Sanchez Alberto , de Castro Angel , Martínez-García M. Sofía
Published: October 2019

The use of Hardware-in-the-Loop (HIL) systems implemented in Field Programmable Gate Arrays (FPGAs) is constantly increasing because of its advantages compared to traditional simulation techniques. This increase in usage has caused new challenges related to the improvement of their performance and features like the number of output channels, while the price of HIL systems is diminishing. At present, the use of low-speed Digital-to-Analog Converters (DACs) is starting to be a commercial possibility because of two reasons. One is their lower price and the other is their lower pin count, which determines the number and price of the FPGAs that are necessary to handle those DACs. This paper compares four filtering approaches for providing suitable data to low-speed DACs, which help to filter high-speed input signals, discarding the need of using expensive high-speed DACS, and therefore decreasing the total cost of HIL implementations. Results show that the selection of the appropriate filter should be based on the type of the input waveform and the relative importance of the dynamics versus the area.
Incremental Capacity Curve Peak Points-Based Regression Analysis for the State-of-Health Prediction of a Retired LiNiCoAlO2 Series/Parallel Configured Battery Pack
Authors: Lee Hyunjun , Park Jounghu , Kim Jonghoon
Published: October 2019

To recycle retired series/parallel battery packs, it is necessary to know their state-of-health (SOH) correctly. Unfortunately, voltage imbalances between the cells occur repeatedly during discharging/charging. The voltage ranges for the discharge/charge of a retired series/parallel battery pack are reduced owing to the voltage imbalances between the cells. To determine the accurate SOH of a retired series/parallel battery pack, it is necessary to calculate the total discharge capacity using fully discharging/charging tests. However, a fully discharging/charging test is impossible owing to the reduced voltage range. The SOH of a retired series/parallel battery pack with a voltage imbalance should be estimated within the reduced discharging/charging voltage range. This paper presents a regression analysis of the peak point in the incremental capacity (IC) curve from the fresh state to a 100-cycle aging state. Moreover, the SOH of the considered retired series/parallel battery pack was estimated using a regression analysis model. The error in the SOHs of the retired series/parallel battery pack and linear regression analysis model was within 1%, and hence a good accuracy is achieved.
Heat-Absorbing Capacity of High-Heat-Flux Components in Nuclear Fusion Reactors
Authors: Lee Namkyu , Kim Beom Seok , Moon Hokyu , Lim Joon-Soo , Cho Hyung Hee
Published: October 2019

Nuclear fusion energy is a solution to the substitution of fossil fuels and the global energy deficit. However, among the several problems encountered for realizing a nuclear fusion reactor, the divertor presents difficulties due to the tremendous heat flux (~10 MW/m2) from high-temperature plasma. Also, neutrons produce additional heat (~17.5 MW/m3) from collisions with the materials’ atoms. This may lead to unexpected effects such as thermal failure. Thus, a comprehensive investigation on the divertor module is needed to determine the heat-absorbing capacity of the divertor module so to maintain the effect of incident heat flux. In this study, using an analytical approach and a simulation, the quantitative effect of heat generation on the thermophysical behavior, such as temperature and thermal stress, was analyzed while maintaining the incident heat flux. Then, a correlated equation was derived from the thermal design criteria, namely, the maximum thimble temperature and the safety factor at the vulnerable point. Finally, on the basis of the thermal design criteria, the heat-absorbing capacity of a nuclear fusion reactor in operating conditions was determined. This study contributes to the understanding of the divertor’s effects in nuclear fusion reactors for high-heat-flux and high-temperature applications.
Sensorless-MTPA Control of Permanent Magnet Synchronous Motor Based on an Adaptive Sliding Mode Observer
Authors: Ye Mengting , Shi Tingna , Wang Huimin , Li Xinmin , Xia Changliang
Published: October 2019

Different from the traditional method of the interior permanent magnet synchronous motor (IPMSM), the sensorless maximum torque per ampere (MTPA) control scheme in this paper does not need two observers for rotor position and d-q axis inductances, respectively. It only needs an adaptive sliding mode observer (ASMO) based on the extended flux (EF) to realize double-loop control and MTPA operation simultaneously. The adaptive mechanism of rotor speed is designed to ensure stability of the ASMO. The rotor position and the difference between d-axis and q-axis inductances are obtained from the estimated EF to acquire the MTPA points when the position sensor of the IPMSM is absent. The proposed scheme is realized on a 20kW IPMSM where the sensorless control performance and the MTPA control performance are tested. The effectiveness of the proposed method is verified by the experiment results.
Photovoltaic Energy Yield Improvement in Two-Stage Solar Microinverters
Authors: Chub Andrii , Vinnikov Dmitri , Stepenko Serhii , Liivik Elizaveta , Blaabjerg Frede
Published: October 2019

The focus in this paper is on the two-stage photovoltaic (PV) microinverters using a buck-boost dc/dc front-end converter. Wide input voltage range of the front-end converter enables operation under shaded conditions but results in mediocre performance in the typical voltage range. These microinverters can be controlled with either fixed or variable dc-link voltage control methods. The latter improves the converter efficiency considerably in the range of the most probable maximum power point (MPP) locations. However, the buck-boost operation of the front-end converter results in noticeable variations of the efficiency across the input voltage range. As a result, conventional weighted efficiency metrics cannot be used to predict annual energy productions by the microinverters. This paper proposes a new methodology for the estimation of annual energy production based on annual profiles of the solar irradiance and ambient temperature. Using this methodology, quantification of the annual energy production is provided for two geographical locations.
A Protection System for Improved Ring-Bus DC Microgrids
Authors: Zhang Zhiming , Chen Qing , Xie Ranran , Zheng Yi
Published: October 2019

An improved system structure and a corresponding protection system are proposed in this paper, which aims at providing future DC microgrids with suitable protection ideas. At first, the ring-bus system is adjusted to balance the system control and protection and make the system more conventional for the equipment expansion. In addition, based on this structure, a protection system is established. It consists of two parts, which are local protection and pilot centralized protection. The local protection is designed for protecting the vulnerable power electronic components in converters and the pilot protection is mainly proposed for the fault isolation. The combination between two parts makes the whole system overcome the contradiction between protection speed and reliability and the method also takes the protection suitability into consideration. Finally, all the methods are verified by the simulation system based on the PSCAD /EMTDC.
Alternative Methodology to Calculate the Directional Characteristic Settings of Directional Overcurrent Relays in Transmission and Distribution Networks
Authors: Jaramillo Serna José de Jesús , López-Lezama Jesús M.
Published: October 2019

When addressing the problem of calculating the settings for directional overcurrent elements, the focus is usually the determination of the pickup, time dial and operating characteristic, in order to ensure proper selectivity with adjacent protection elements, thus limiting the problem related to the settings calculation of the direction determination characteristic to the application of typical settings and general guidelines, which cannot provide a reliable measure of the suitability of such settings. The present article describes in detail an alternative methodology for determining these settings, based on a characterization of the power system where the directional protection is to be applied, through the performance of a detailed short-circuit sensitivity analysis. From this, an optimization problem is formulated and solved to obtain the main settings shaping the direction determination characteristic, and then, a series of variables are used to measure the performance of the obtained settings, and even to improve it. The obtained results show the advantages of the application of the proposed methodology over the traditional methodology, based on typical settings and general guidelines, pointing out the risks of using the later.
Monitoring, Assessment and Management of Forest Insect Pests and Diseases
Authors: Choi Won Il , Park Young-Seuk
Published: October 2019

Forest pests are one of the most important factors disturbing forest ecosystems, by impacting forestry economy, ecosystem services, biodiversity, and sustainable ecosystem management. Monitoring the occurrence of forest pests offers clues to understand their impacts on the forest ecosystem and develop a sustainable ecosystem management strategy. This special issue is designed to create a better understanding of the changes and impacts of forest pests according to forest changes, caused by natural or anthropogenic causes. There are 13 papers published in this special issue, covering several issues concerning forest pests. Two of the papers reviewed the changes in forest pests in Korea or Poland. The remaining twelve papers covered issues concerning the monitoring, assessment, and management of forest pests. Through this special issue, we expect to contribute towards the improvement of our knowledge of the structures and processes in forest ecosystems relating to forest pests and fundamental information for the effective management of forest pests.
Fall Ethephon Application Enhances the Freezing Tolerance of Magnolia wufengensis During Overwintering
Authors: Duan Xiaojing , Cai Chuheng , Yang Yang , Chen Faju , Sang Ziyang , Ma Luyi
Published: October 2019

Magnolia wufengensis L.Y. Ma et L. R. Wang, a rare species which has been introduced and cultivated from southern China to northern China, frequently suffers from freezing injuries. To figure out the influence of ethephon (ETH) application on the cold tolerance during the natural overwintering of M. wufengensis, one-year shoots subjected to ethephon application at four concentrations (0, 700, 1000, and 1500 mg·L−1) were collected to measure the physiological and biochemical changes from September 2017 to 1 April 2018. The fall ETH application increased the freezing tolerance of M. wufengensis, and the optimum concentration for M. wufengensis was 1000 mg·L−1 (T2), which not only improved the shoot freezing tolerance by 1.4 times, but also led to a 25.0% faster cold acclimation rate and 13.7% slower de-acclimation rate. Moreover, 1000 mg·L−1 ETH delayed bud-burst in the spring by 10. 7 d, which was helpful for effectively avoiding cold spells in the spring, and improved the rates of bud survival by 47.1%. The improved freezing tolerance under exogenous ETH application was associated with an increased dehydration and accumulation of proline content. It seems that exogenous ETH application may be used on M. wufengensis grown in northern China to protect against freezing tolerance during the overwintering period.
Geographical Distribution and Environmental Correlates of Eleutherosides and Isofraxidin in Eleutherococcus senticosus from Natural Populations in Forests at Northeast China
Authors: Guo Shenglei , Wei Hongxu , Li Junping , Fan Ruifeng , Xu Mingyuan , Chen Xin , Wang Zhenyue
Published: October 2019

Non-wood forest products (NWFPs) derived from understory plants are attracting attention about sustainable forestry development. Geographical distribution and climate correlates of bioactive compounds are important to the regional management for the natural reserves of medical plants in forests. In this study, we collected Eleutherococcus senticosus individuals from 27 plots to map the special distribution of concentrations of eleutheroside B, eleutheroside E, and isofraxidin in forests of Northeast China. Compound concentrations in both aerial and underground organs were further detected for relationships with the average of 20-year records of temperature, precipitation, and relative humidity (RH). We found higher shoot eleutheroside B concentration in populations in northern and low-temperature regions (R = −0.4394; P = 0.0218) and in eastern and high-RH montane forests (R = 0.5003; P = 0.0079). The maximum-likelihood regression indicated that both RH (Pr > Chi-square, 0.0201) and longitude (Pr > Chi-square, 0.0026) had positive contributions to eleutheroside B concentration in roots, but precipitation had strongly negative contributions to the concentrations of eleutheroside E (Pr > Chi-square, 0.0309) and isofraxidin (Pr > Chi-square, 0.0014) in roots. Both geography and climate factors had effects on the special distribution of medical compounds in E. senticosus plants in natural populations in Northeast China. The management of NWFP plants at the regional scale should consider effects from climatic geography.
Shifts of the Mean Centers of Potential Vegetation Ecosystems under Future Climate Change in Eurasia
Authors: Fan Zemeng , Fan Bin
Published: October 2019

Climate change dominantly controls the spatial distributions of potential vegetation ecosystems; the shift trends in the mean centers of potential vegetation ecosystems could be used to explain their responses to climate change. In terms of the climate observation data of Eurasia for the period from 1981 to 2010 and the climate scenario data for the period from 2011 to 2100 under the three Representative Concentration Pathways (RCPs) scenarios of RCP2.6, RCP4.5 and, RCP8.5, which were released by the Coupled Model Intercomparison Project Phase 5 (CMIP5), the Holdridge Life Zone (HLZ) ecosystem model was improved to quantitatively classify the potential vegetation types, and the shift model of mean center was adopted to compute the trends in the spatiotemporal shifts of potential vegetation types in Eurasia. The results showed that the mean centers of the major potential vegetation ecosystems would be distributed in the central and southern parts of Eurasia. Under the RCP2.6, RCP4.5, and RCP8.5 scenarios, the potential shift distances of the mean centers of the vegetation types under the RCP8.5 scenario would be the largest, and those of the polar/nival area, subpolar/alpine moist tundra, warm temperate dry forest, subtropical moist forest, cool temperate moist forest, cool temperate wet forest, subtropical wet forest, subtropical thorn woodland, warm temperate moist forest and subtropical dry forest would be larger than those in the other potential vegetation types in Eurasia. Moreover, the shift directions of the mean centers of the major potential vegetation types would generally shift northward, and subtropical dry forest, warm temperate moist forest and subpolar/alpine moist tundra would be the most sensitive to change among all vegetation types under the three scenarios for the period from 2011 to 2100.
Ectopic Expression of Multiple Chrysanthemum (Chrysanthemum × morifolium) R2R3-MYB Transcription Factor Genes Regulates Anthocyanin Accumulation in Tobacco
Authors: Hong Yan , Li Mengling , Dai Silan
Published: October 2019

The generation of chrysanthemum (Chrysanthemum × morifolium) flower color is mainly attributed to the accumulation of anthocyanins. In the anthocyanin biosynthetic pathway in chrysanthemum, although all of the structural genes have been cloned, the regulatory function of R2R3-MYB transcription factor (TF) genes, which play a crucial role in determining anthocyanin accumulation in many ornamental crops, still remains unclear. In our previous study, four light-induced R2R3-MYB TF genes in chrysanthemum were identified using transcriptomic sequencing. In the present study, we further investigated the regulatory functions of these genes via phylogenetic and alignment analyses of amino acid sequences, which were subsequently verified by phenotypic, pigmental, and structural gene expression analyses in transgenic tobacco lines. As revealed by phylogenetic and alignment analyses, CmMYB4 and CmMYB5 were phenylpropanoid and flavonoid repressor R2R3-MYB genes, respectively, while CmMYB6 was an activator of anthocyanin biosynthesis, and CmMYB7 was involved in regulating flavonol biosynthesis. Compared with wild-type plants, the relative anthocyanin contents in the 35S:CmMYB4 and 35S:CmMYB5 tobacco lines significantly decreased (p < 0.05), while for 35S:CmMYB6 and 35S:CmMYB7, the opposite result was obtained. Both in the 35S:CmMYB4 and 35S:CmMYB5 lines, the relative expression of several anthocyanin biosynthetic genes in tobacco was significantly downregulated (p < 0.05); on the contrary, several genes were upregulated in the 35S:CmMYB6 and 35S:CmMYB7 lines. These results indicate that CmMYB4 and CmMYB5 negatively regulate anthocyanin biosynthesis in chrysanthemum, while CmMYB6 and CmMYB7 play a positive role, which will aid in understanding the complex mechanism regulating floral pigmentation in chrysanthemum and the functional divergence of the R2R3-MYB gene family in higher plants.
Location Modeling of Final Palaeolithic Sites in Northern Germany
Authors: Hamer Wolfgang B. , Knitter Daniel , Grimm Sonja B. , Serbe Benjamin , Eriksen Berit Valentin , Nakoinz Oliver , Duttmann Rainer
Published: October 2019

Location modeling, both inductive and deductive, is widely used in archaeology to predict or investigate the spatial distribution of sites. The commonality among these approaches is their consideration of only spatial effects of the first order (i.e., the interaction of the locations with the site characteristics). Second-order effects (i.e., the interaction of locations with each other) are rarely considered. We introduce a deductive approach to investigating such second-order effects using linguistic hypotheses about settling behavior in the Final Palaeolithic. A Poisson process was used to simulate a point distribution using expert knowledge of two distinct hunter–gatherer groups, namely, reindeer hunters and elk hunters. The modeled points and point densities were compared with the actual finds. The G-, F-, and K-function, which allow for the identification of second-order effects of varying intensity for different periods, were applied. The results reveal differences between the two investigated groups, with the reindeer hunters showing location-related interaction patterns, indicating a spatial memory of the preferred locations over an extended period of time. Overall, this paper shows that second-order effects occur in the geographical modeling of archaeological finds and should be taken into account by using approaches such as the one presented in this paper.
Toxoplasma gondii Serointensity and Seropositivity: Heritability and Household-Related Associations in the Old Order Amish
Authors: Duffy Allyson R. , O’Connell Jeffrey R. , Pavlovich Mary , Ryan Kathleen A. , Lowry Christopher A. , Daue Melanie , Raheja Uttam K. , Brenner Lisa A. , Markon André O. , Punzalan Cecile M. , Dagdag Aline , Hill Dolores E. , Pollin Toni I. , Seyfang Andreas , Groer Maureen W. , Mitchell Braxton D. , Postolache Teodor T.
Published: October 2019

Toxoplasma gondii (T. gondii) is an intracellular parasite infecting one third of the world’s population. Latent T. gondii infection has been associated with mental illness, including schizophrenia and suicidal behavior. T. gondii IgG antibody titers were measured via ELISA. The heritability of T. gondii IgG was estimated using a mixed model that included fixed effects for age and sex and random kinship effect. Of 2017 Old Order Amish participants, 1098 had positive titers (54.4%). The heritability for T. gondii serointensity was estimated to be 0.22 (p = 1.7 × 10−8 and for seropositivity, it was estimated to be 0.28 (p = 1.9 × 10−5). Shared household environmental effects (i.e., household effects) were also determined. Household effects, modeled as a random variable, were assessed as the phenotypic covariance between any two individuals who had the same current address (i.e., contemporaneous household), and nuclear household (i.e., the phenotypic covariance between parents and children only, not other siblings or spouses). Household effects did not account for a significant proportion of variance in either T. gondii serointensity or T. gondii seropositivity. Our results suggest a significant familial aggregation of T. gondii serointensity and seropositivity with significant heritability. The shared household does not contribute significantly to family aggregation with T. gondii, suggesting that there are possible unmeasured non-household shared and non-shared environmental factors that may play a significant role. Furthermore, the small but significant heritability effects justify the exploration of genetic vulnerability to T. gondii exposure, infection, virulence, and neurotropism.
A Comparative Study of Water Quality and Human Health Risk Assessment in Longevity Area and Adjacent Non-Longevity Area
Authors: Yu Jiawen , Zhou Jinlong , Long Aihua , He Xinlin , Deng Xiaoya , Chen Yunfei
Published: October 2019

A longevity area in Xinjiang, China and an adjacent non-longevity area both have similar climatic and hydrogeological conditions, and the residents of the two control groups have similar ethnic composition, diets and lifestyles. This study investigated if differences in groundwater quality between the longevity area and the non-longevity area are associated with the health of residents in the two control groups. In order to quantitatively describe the groundwater quality of the two control groups and its influence on human health, the Fuzzy Comprehensive Evaluation Method (FCEM) was used to compare and assess the overall water environment of the two control groups. Furthermore, the human health risk of groundwater for the two control groups was assessed using the Health Risk Assessment Model recommended by the U.S. Environmental Protection Agency (USEPA). Results showed that the overall water environment categories for the longevity area and non-longevity area are moderate quality (grade III) and very poor quality (grade V), respectively. The main health risk in the longevity area water environment is the non-carcinogenic risk (HQLLV) caused by Cl−. The main health risks in the non-longevity area water environment are the non-carcinogenic risk (HQCA) caused by Cl− and the carcinogenic risk (RiskCA) caused by As. The total health risk (HRall) caused by over-standard inorganic pollutants in the water environment of the non-longevity area is 3.49 times higher than that of the longevity area. In addition, the study showed that the water environment pollution downstream of the Keriya River is conjunctively caused by agricultural activities and domestic sewage. The overall water environment of the longevity area is more conducive to the health-longevity of residents than the non-longevity area.
Regulatory Policies for Alcohol, other Psychoactive Substances and Addictive Behaviours: The Role of Level of Use and Potency. A Systematic Review
Authors: Rehm Jürgen , Crépault Jean-François , Hasan Omer , Lachenmeier Dirk , Room Robin , Sornpaisarn Bundit
Published: October 2019

The object of this contribution based on a systematic review of the literature is to examine to what degree the level of use and potency play a role in regulatory policies for alcohol, other psychoactive substances and gambling, and whether there is an evidence base for this role. Level of use is usually defined around a behavioural pattern of the user (for example, cigarettes smoked per day, or average ethanol use in grams per day), while potency is defined as a property or characteristic of the substance. For all substances examined (alcohol, tobacco, opioids, cannabis) and gambling, both dimensions were taken into consideration in the formulation of most regulatory policies. However, the associations between both dimensions and regulatory policies were not systematic, and not always based on evidence. Future improvements are suggested.
A Proteomics-Based Approach Reveals Differential Regulation of Urine Proteins between Metabolically Healthy and Unhealthy Obese Patients
Authors: Benabdelkamel Hicham , Masood Afshan , Okla Meshail , Al-Naami Mohammed Y. , Alfadda Assim A.
Published: October 2019

Metabolic dysfunction associated with obesity threatens to inundate health care resources by increasing the incidences of obesity-related diseases. The aim of the present study was to investigate the changes in the urinary proteome of 18 individuals classified into metabolically healthy obese (MHO) and metabolically unhealthy obese (MUHO) patients. Proteome analysis was performed using the two-dimensional difference in gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS). Upon analysis, a total of 54 proteins were found to be affected with ≥1.5-fold change (ANOVA, p ≤ 0.05), of which 44 proteins were upregulated and 10 proteins were downregulated. These differentially abundant proteins were related to nuclear factor κB (NF-κB) and p38 mitogen-activated protein (MAP) kinase pathways and were involved in cellular compromise, inflammatory response, and cancer. Proteins involved in inflammation (fibrinogen alpha (FIBA), serotransferrin (TRFE, and kininogen-1 (KNG1)) and insulin resistance (ADP-ribosylation factor (ARF)-like protein 15 (ARL15) and retinol-binding protein 4 (RET4)) were found to be significantly increased in the urine samples of MUHO compared to MHO patients. Investigating the effects of obesity on urinary proteins can help in developing efficient diagnostic procedures for early detection and prevention of obesity-related complications.
A Generalized Kinetic Model for Coupling between Stepping and ATP Hydrolysis of Kinesin Molecular Motors
Authors: Xie Ping , Guo Si-Kao , Chen Hong
Published: October 2019

A general kinetic model is presented for the chemomechanical coupling of dimeric kinesin molecular motors with and without extension of their neck linkers (NLs). A peculiar feature of the model is that the rate constants of ATPase activity of a kinesin head are independent of the strain on its NL, implying that the heads of the wild-type kinesin dimer and the mutant with extension of its NLs have the same force-independent rate constants of the ATPase activity. Based on the model, an analytical theory is presented on the force dependence of the dynamics of kinesin dimers with and without extension of their NLs at saturating ATP. With only a few adjustable parameters, diverse available single molecule data on the dynamics of various kinesin dimers, such as wild-type kinesin-1, kinesin-1 with mutated residues in the NLs, kinesin-1 with extension of the NLs and wild-type kinesin-2, under varying force and ATP concentration, can be reproduced very well. Additionally, we compare the power production among different kinesin dimers, showing that the mutation in the NLs reduces the power production and the extension of the NLs further reduces the power production.