bluuebunny/crossref_metadata_2025 huggingface dataset:
Pathways and Timescales of Connectivity Around the Antarctic Continental Shelf
Authors: Dawson Hannah R. S. , Morrison Adele K. , England Matthew H. , Tamsitt Veronica
Published: January 2023

The Antarctic Slope Current (ASC) and Antarctic Coastal Current advect heat, freshwater, nutrients, and biological organisms westward around the Antarctic margin, providing a connective link between different sectors of the continental shelf. Yet the strength and pathways of connectivity around the continent, and the timescales of advection, remain poorly understood. We use daily velocity fields from a global high‐resolution ocean‐sea ice model, combined with Lagrangian particle tracking, to shed light on these timescales and improve our understanding of circumpolar connectivity around Antarctica. Virtual particles were released along vertical transects over the continental shelf every 5 days for a year and were tracked forward in time for 21 years. Analysis of the resulting particle trajectories highlights that the West Antarctic sector has widespread connectivity with all regions of the Antarctic shelf. Advection around the continent is typically rapid with peak transit times of 1–5 years for particles to travel 90° of longitude downstream. The ASC plays a key role in driving connectivity in East Antarctica and the Weddell Sea, while the Coastal Current controls connectivity in West Antarctica, the eastern Antarctic Peninsula, and along the continental shelf east of Prydz Bay. Connectivity around the shelf is impeded in two main locations, namely, the tip of the Antarctic Peninsula and Cape Adare in the Ross Sea, where significant export of water from the continental shelf is found. These findings help to understand the locations and timescales over which anomalies, such as meltwater from the Antarctic Ice Sheet, can be redistributed downstream.
Mechanisms of Heat Flux Across the Southern Greenland Continental Shelf in 1/10° and 1/12° Ocean/Sea Ice Simulations
Authors: Morrison Theresa J. , Dukhovskoy Dmitry S. , McClean Julie L. , Gille Sarah T. , Chassignet Eric P.
Published: January 2023

The presence of warm Atlantic water on the Greenland continental shelf has been connected to the accelerated melting of the Greenland Ice Sheet, particularly in the southwest and southeast shelf regions. Results from two high‐resolution coupled ocean‐sea ice simulations that utilized either the 1/10° Parallel Ocean Program (POP) or the 1/12° HYbrid Coordinate Ocean Model (HYCOM) are used to understand the flux of heat on and off the southern Greenland shelf. The analysis reveals that the region of greatest heat flux onto the shelf is southeast Greenland. On the southwestern shelf, heat is mainly exported from the shelf to the interior basins. We identify differences in the shelf break current structure and on‐shelf heat content between the two simulations. Just south of the Denmark Strait, there is a seasonally persistent pattern of multi‐day variability in the cross‐shelf heat flux in both simulations. In the POP simulation, this high‐frequency signal results in net on‐shore heat flux. In the HYCOM simulation, the signal is weaker and results in net off‐shelf heat flux. This variability is consistent with Denmark Strait Overflow eddies traveling along the shelf break.
Driving Forces of Extreme Updrafts Associated With Convective Bursts in the Eyewall of a Simulated Tropical Cyclone
Authors: Qin Nannan , Wu Liguang , Liu Qingyuan , Zhou Xingyang
Published: January 2023

Many studies have indicated that convective bursts (CBs) are closely related to tropical cyclone (TC) intensification, but few studies have been conducted on the mechanisms that control the formation and evolution of CBs. In this study, the 1‐min output data of a simulated TC are used to understand the convective extreme updrafts of CBs in the TC eyewall. Three different reference states including the local square‐area mean (LAM), the lower wavenumber‐components mean (WNM), and the arc‐area mean (ArcM) are used to calculate the driving forces of convective extreme updrafts. Contrary to the WNM and ArcM reference states, the LAM reference state struggles to capture realistic basic state structures. The LAM reference state can be modified through the inclusion of the mean hydrometeor mixing ratio in the basic state to produce physically consistent forcing in relation to the simulated convective extreme updrafts. The simulated convective extreme updrafts in the TC eyewall exhibit two peaks at middle and upper levels, respectively, since the effect of hydrometeor loading, decelerates the air parcels between the updraft maxima. While the positive buoyancy makes air parcels in the CBs accelerate at middle levels, in agreement with previous studies, it is found that, at the upper levels, both the positive buoyancy and the upward vertical perturbation pressure gradient force accelerate the air parcels. This study suggests that the vertical perturbation pressure gradient force also plays an important role in the formation of CBs in the TC eyewall.
The Ratio Distribution of Evapotranspiration to Precipitation well Related With Soil Thickness in Karst Watershed of Southwest China
Authors: Zhang Rongfei , Xu Xianli , Guo Jingsong , Sheng Zhuping
Published: January 2023

Evapotranspiration (ET) is predominant variable for water management in various types of ecosystems, and it has been intensively studied through in situ measuring and modeling methods. However, it is challenging to measure actual ET and upscale it to regional level for karst landscapes, where aquifers typically show remarkable and rapid responses to precipitation events. There is an urgent need to quantify water fluxes to provide reliable evidence for the protection and sustainable management of karst water resources. Therefore, in this study, five plots were built to observe actual ET based on Thermal Dissipation Probes, redesigned ventilated chamber and in‐site microlysimeters in a karst watershed in southwest China. Then, three models (Penman‐Monteith‐Leurning, PML; Remote Sensing‐Priestley and Taylor; and Hargreaves) were constructed to upscale ET estimation to the regional level based on Landsat‐8 images and MODIS data. The results showed that: (a) The PML model performed better than other models (p < 0.01) with higher R2 values (0.72 for MODIS images and 0.87 for Landsat‐8 images); (b) Daily ET exhibited significant seasonal variability and different spatial distribution, and yearly ET exhibited well related with soil thickness (R2 = 0.58); (c) Daily ET had a slightly positive correlation with DEM, however, had a negative correlation with ground temperature. This study is helpful to improve accuracy of measured and estimated ET by combining remote sensing data, and it has important implications for upscaling it to regional level. It suggests that ET is strongly regulated by constant supply of water, vegetation coverage, and available energy in karst catchments.
Stable Water Isotopes as an Indicator of Surface Water Intrusion in Shallow Aquifer Wells: A Cold Climate Perspective
Authors: Yapiyev Vadim , Rossi Pekka M. , Ala‐Aho Pertti , Marttila Hannu
Published: January 2023

Groundwater in shallow aquifers is commonly used for community water supply in cold climates. Shallow groundwaters are inherently susceptible to contamination from land‐use and surface water intrusion threatening drinking water usage. We used a large‐scale snapshot data set of stable water isotopes from shallow glaciofluvial aquifers used for drinking water supply in Northern Finland to assess surface water intrusion risks and recharge conditions. This data set was supplemented by long‐term stable water isotope precipitation data, Geographic Information System proximity analysis and multivariate statistics. The isotope analysis suggest that a warm season contributes about 60% to the total annual precipitation in the region. This is reflected in the aquifers isotopic composition as it represents an approximately equal mixture of warm and cold season precipitation. Groundwater isotope data normalized to precipitation inputs by line‐conditioned excess (lc‐excess) was used to flag the water supply wells impacted by surface water intrusions. The proximity analysis showed some of the wells may be affected by intrusions from gravel pit ponds, lakes and peatland drainage. On the larger scale, the wells in coastal areas were more likely to have evaporated water (intrusion) compared to inland regions of Northern Finland due to lower water availability and the presence of man‐made structures. This application of stable water isotopes with lc‐excess is a useful approach not only for recharge studies but also within water management for supply well surface water contamination risk assessment.
Labour market participation after spinal cord injury. A register-based cohort study
Authors: Halvorsen Annette , Steinsbekk Aslak , Leiulfsrud Annelie Schedin , Post Marcel W. M. , Biering-Sørensen Fin , Pape Kristine
Published: January 2023

Study design

A register based cohort study.

Objectives

To investigate labour market participation following spinal cord injury (SCI) and to describe the impact of personal and SCI characteristics.

Setting

Norway.

Methods

Persons registered with SCI in the Norwegian SCI registry 2011–2017, and matched reference individuals without SCI from the general population (named controls) were followed for up to six years after injury using national registry data on employment, education, income, and social security benefits. Main measures of labour market participation were: (1) Receiving any amount of pay for work, and (2) Receiving sickness and disability benefits.

Results

Among the 451 persons with SCI (aged 16–66 years and working before injury), the estimated percentages receiving pay for work and sickness and disability benefits in the sixth years after injury were 63% (95% CI 57–69) and 67% (95% CI 61–72).

Corresponding percentages for the controls (n = 1791) were 91% (95% CI 90–93) for receiving pay for work and 13% (95% CI 12–15) for receiving sickness and disability benefits. Among persons with SCI, less severe neurological outcome, higher level of education, younger age at injury, and a stronger pre-injury attachment to employment (higher employment income, having an employer, less receipt of benefits), were associated with higher labour market participation.

Conclusion

SCI substantially decreased labour market participation up to six years after injury compared to matched controls. Even if a relatively large proportion of persons with SCI remained in some degree of work activity, more than half did so in combination with receiving benefits.
Reciprocal regulation of LINC00941 and SOX2 promotes progression of esophageal squamous cell carcinoma
Authors: Lu Jun-Tao , Yan Zhao-Yang , Xu Tong-Xin , Zhao Fan , Liu Lei , Li Fei , Guo Wei
Published: January 2023

LINC00941 is a novel long noncoding RNA (lncRNA) and emerging as an important factor in cancer development. However, the exact function and relative regulatory mechanism of LINC00941 in carcinogenesis of esophageal squamous cell carcinoma (ESCC) remain to be further clarified. The present study was to investigate the expression level, functions, and mechanisms of LINC00941 in ESCC tumorigenesis. LINC00941 was significantly upregulated in ESCC, and upregulated LINC00941 was correlated with dismal patient outcomes. LINC00941 functioned as an oncogene by promoting cells proliferation, stemness, migration, and invasion in ESCC. In terms of mechanisms, SOX2 could bind directly to the promoter region of LINC00941 and activate its transcription. In turn, LINC00941 upregulated SOX2 through interacting with interleukin enhancer binding factor 2 (ILF2) and Y-box binding protein 1 (YBX1) at the transcriptional and post-transcriptional levels. LINC00941 recruited ILF2 and YBX1 to the promoter region of SOX2, leading to upregulation of the transcription of SOX2. Moreover, LINC00941 could promote the binding ability of ILF2 and YBX1 on mRNA of SOX2 and further stabilize SOX2 mRNA. Therefore, LINC00941 contributed to the malignant behaviors of ESCC cells via the unrestricted increase in SOX2 expression. In conclusion, our data indicate that LINC00941 exacerbates ESCC progression through forming a LINC00941-ILF2/YBX1-SOX2 positive feedback loop, and LINC00941 may be a promising prognostic and therapeutic target for ESCC.
Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection
Authors: Zhao Shuangshuang , Xu Qiuping , Cui Yanqin , Yao Su , Jin Sihui , Zhang Qian , Wen Zeyu , Ruan Haihua , Liang Xin , Chao Yanjie , Gong Sitang , Sansonetti Philippe , Wei Ke , Tang Hong , Jiu Yaming
Published: January 2023

A variety of intracellular bacteria modulate the host cytoskeleton to establish subcellular niches for replication. However, the role of intermediate filaments, which are crucial for mechanical strength and resilience of the cell, and in bacterial vacuole preservation remains unclear. Here, we show thatSalmonellaeffector SopB reorganizes the vimentin network to form cage-like structures that surroundSalmonella-containing vacuoles (SCVs). Genetic removal of vimentin markedly disrupts SCV organization, significantly reduces bacterial replication and cell death. Mechanistically, SopB uses its N-terminal Cdc42-binding domain to interact with and activate Cdc42 GTPase, which in turn recruits vimentin around SCVs. A high-content imaging-based screening identified that MEK1/2 inhibition led to vimentin dispersion. Our work therefore elucidates the signaling axis SopB-Cdc42-MEK1/2 as mobilizing host vimentin to maintain concrete SCVs and identifies a mechanism contributing toSalmonellareplication. Importantly, Trametinib, a clinically-approved MEK1/2 inhibitor identified in the screen, displayed significant anti-infection efficacy againstSalmonellaboth in vitro and in vivo, and may provide a therapeutic option for treating drug-tolerant salmonellosis.
Ligand recognition mechanism of the human relaxin family peptide receptor 4 (RXFP4)
Authors: Chen Yan , Zhou Qingtong , Wang Jiang , Xu Youwei , Wang Yun , Yan Jiahui , Wang Yibing , Zhu Qi , Zhao Fenghui , Li Chenghao , Chen Chuan-Wei , Cai Xiaoqing , Bathgate Ross A .D. , Shen Chun , Eric Xu H. , Yang Dehua , Liu Hong , Wang Ming-Wei
Published: January 2023

Members of the insulin superfamily regulate pleiotropic biological processes through two types of target-specific but structurally conserved peptides, insulin/insulin-like growth factors and relaxin/insulin-like peptides. The latter bind to the human relaxin family peptide receptors (RXFPs). Here, we report three cryo-electron microscopy structures of RXFP4–Gi protein complexes in the presence of the endogenous ligand insulin-like peptide 5 (INSL5) or one of the two small molecule agonists, compound 4 and DC591053. The B chain of INSL5 adopts a single α-helix that penetrates into the orthosteric pocket, while the A chain sits above the orthosteric pocket, revealing a peptide-binding mode previously unknown. Together with mutagenesis and functional analyses, the key determinants responsible for the peptidomimetic agonism and subtype selectivity were identified. Our findings not only provide insights into ligand recognition and subtype selectivity among class A G protein-coupled receptors, but also expand the knowledge of signaling mechanisms in the insulin superfamily.
Piezo robotic hand for motion manipulation from micro to macro
Authors: Zhang Shijing , Liu Yingxiang , Deng Jie , Gao Xiang , Li Jing , Wang Weiyi , Xun Mingxin , Ma Xuefeng , Chang Qingbing , Liu Junkao , Chen Weishan , Zhao Jie
Published: January 2023

Multiple degrees of freedom (DOFs) motion manipulation of various objects is a crucial skill for robotic systems, which relies on various robotic hands. However, traditional robotic hands suffer from problems of low manipulation accuracy, poor electromagnetic compatibility and complex system due to limitations in structures, principles and transmissions. Here we present a direct-drive rigid piezo robotic hand (PRH) constructed on functional piezoelectric ceramic. Our PRH holds four piezo fingers and twelve motion DOFs. It achieves high adaptability motion manipulation of ten objects employing pre-planned functionalized hand gestures, manipulating plates to achieve 2L (linear) and 1R (rotary) motions, cylindrical objects to generate 1L and 1R motions and spherical objects to produce 3R motions. It holds promising prospects in constructing multi-DOF ultra-precision manipulation devices, and an integrated system of our PRH is developed to implement several applications. This work provides a new direction to develop robotic hand for multi-DOF motion manipulation from micro scale to macro scale.
Target templates and the time course of distractor location learning
Authors: Hanne Aylin A. , Tünnermann Jan , Schubö Anna
Published: January 2023

When searching for a shape target, colour distractors typically capture our attention. Capture is smaller when observers search for a fixed target that allows for a feature-specific target template compared to a varying shape singleton target. Capture is also reduced when observers learn to predict the likely distractor location. We investigated how the precision of the target template modulates distractor location learning in an additional singleton search task. As observers are less prone to capture with a feature-specific target, we assumed that distractor location learning is less beneficial and therefore less pronounced than with a mixed-feature target. Hierarchical Bayesian parameter estimation was used to fit fine-grained distractor location learning curves. A model-based analysis of the time course of distractor location learning revealed an effect on the asymptotic performance level: when searching for a fixed-feature target, the asymptotic distractor cost indicated smaller distractor interference than with a mixed-feature target. Although interference was reduced for distractors at the high-probability location in both tasks, asymptotic distractor suppression was less pronounced with fixed-feature compared to mixed-feature targets. We conclude that with a more precise target template less distractor location learning is required, likely because the distractor dimension is down-weighted and its salience signal reduced.
Synthesis, antimicrobial activity and molecular docking studies of spiroquinoline-indoline-dione and spiropyrazolo-indoline-dione derivatives
Authors: Gul Melek , Turk Celikoglu Emine , Idil Onder , Tas Gamze , Pelit Emel
Published: January 2023

Spiro[benzo[h]quinoline-7,3′-indoline]diones and spiro[indoline-3,4′-pyrazolo[3,4-b]quinoline]diones were efficiently synthesized via one-pot multi-component reactions under ultrasound-promoted conditions. Spiro[benzo[h]quinoline-7,3′-indoline]dione derivatives were successfully developed by the reaction of isatins, naphthalene-1-amine and 1,3-dicarbonyl compounds. The spiro[indoline-3,4′-pyrazolo[3,4-b]quinoline]dione derivatives were prepared by the reaction of isatins, 5-amino-1-methyl-3-pheylpyrazole, and 1,3-dicarbonyl compounds by using ( ±)-camphor-10-sulfonic acid as a catalyst in H2O/EtOH (3:1 v/v) solvent mixture. The antibacterial activity of the synthesized compounds was evaluated against,Enterococcus faecalis,Staphylococcus aureusandCandida albicans. Compounds 4b, 4h, and 6h showed the strongest antimicrobial activity toward both bacteria. The MIC values of these compounds ranged from 375–3000 µg/mL. The effect of these compounds (4b, 4h, 6h) as a function of applied dose and time was investigated by a kinetic study, and the interaction with these antimicrobial results was simulated by a molecular docking study. We also used the docking approach with Covid-19 since secondary bacterial infections. Docking showed that indoline-quinoline hybrid compounds 4b and 4h exerted the strongest docking binding value against the active sites of 6LU7. In addition, the synthesized compounds had a moderate to good free radical scavenging activity.
Timely sown maize hybrids improve the post-anthesis dry matter accumulation, nutrient acquisition and crop productivity
Authors: Zhiipao R. R. , Pooniya Vijay , Biswakarma Niraj , Kumar Dinesh , Shivay Y. S. , Dass Anchal , Mukri Ganapati , Lakhena K. K. , Pandey R. K. , Bhatia Arti , Govindasamy Prabhu , Burman Anamika , Babu Subhash , Jat R. D. , Dhaka A. K. , Swarnalakshmi Karivaradharajan
Published: January 2023

Delayed sowing of maize hybrids could exacerbate the capability of maximizing the yield potential through poor crop stand, root proliferation, nutrient uptake, and dry matter accumulation coupled with the inadequate partitioning of the assimilates. This study appraised the performance of five recent maize hybrids viz., PMH-1, PJHM-1, AH-4158, AH-4271, and AH-8181 under timely and late sown conditions of the irrigated semi-arid ecologies. Timely sowing had the grain and stover yields advantage of 16–19% and 12–25%, respectively over the late sown maize hybrids. The advanced hybrids AH-4271 and AH-4158 had higher grain yields than the others. During the post-anthesis period, a greater dry matter accumulation and contribution to the grain yield to the tune of 16% and 10.2%, respectively, was observed under timely sown conditions. Furthermore, the nutrient acquisition and use efficiencies also improved under the timely sown. The nutrient and dry matter remobilization varied among the hybrids with AH-4271 and PMH-1 registering greater values. The grain yield stability index (0.85) was highest with AH-4158 apart from the least yield reduction (15.2%) and stress susceptibility index (0.81), while the maximum geometric mean productivity was recorded with the AH-4271 (5.46 Mg ha−1). The hybrids AH-4271 and PJHM-1 exhibited improved root morphological traits, such as root length, biomass, root length density, root volume at the V5 stage (20 days after sowing, DAS) and 50% flowering (53 DAS). It is thus evident that the timely sowing and appropriate hybrids based on stress tolerance indices resulted in greater yields and better utilization of resources.
Synthesis, characterization, and biodegradation studies of new cellulose-based polymers
Authors: Tabaght F. E. , Azzaoui K. , Idrissi A. El , Jodeh S. , Khalaf B. , Rhazi L. , Bellaouchi R. , Asehraou A. , Hammouti B. , Sabbahi R.
Published: January 2023

New cellulose carbamates and cellulose acetate carbamates were prepared by classical addition reaction of isocyanates with alcohols. A Telomerization technique was used to make the grafted molecules strongly anchored and more hydrophobic. These molecules were grafted into cellulose and CA chains, respectively. The structures of the synthesized derivatives were confirmed using Nuclear Magnetic Resonance Spectroscopy, Fourier Transform Infrared and Thermogravimetric Analysis, and their solubility phenomenon was also established, and the carbamate derivatives showed better solubility compared to cellulose. Their ability to biodegrade was investigated, and it was concluded that Cell-P1 and CA-P1 derivatives are more biodegradable than the other samples. These results suggest that the resulting compounds can be used effectively in many useful industrial fields, for instance, eco-friendly food packaging, domains that use materials that are environmentally friendly and sustainable and the development of green chemistry.
Studying the effect of self-selected background music on reading task with eye movements
Authors: Que Ying , Zheng Yueyuan , Hsiao Janet H. , Hu Xiao
Published: January 2023

Using background music (BGM) during learning is a common behavior, yet whether BGM can facilitate or hinder learning remains inconclusive and the underlying mechanism is largely an open question. This study aims to elucidate the effect of self-selected BGM on reading task for learners with different characteristics. Particularly, learners’ reading task performance, metacognition, and eye movements were examined, in relation to their personal traits including language proficiency, working memory capacity, music experience and personality. Data were collected from a between-subject experiment with 100 non-native English speakers who were randomly assigned into two groups. Those in the experimental group read English passages with music of their own choice played in the background, while those in the control group performed the same task in silence. Results showed no salient differences on passage comprehension accuracy or metacognition between the two groups. Comparisons on fine-grained eye movement measures reveal that BGM imposed heavier cognitive load on post-lexical processes but not on lexical processes. It was also revealed that students with higher English proficiency level or more frequent BGM usage in daily self-learning/reading experienced less cognitive load when reading with their BGM, whereas students with higher working memory capacity (WMC) invested more mental effort than those with lower WMC in the BGM condition. These findings further scientific understanding of how BGM interacts with cognitive tasks in the foreground, and provide practical guidance for learners and learning environment designers on making the most of BGM for instruction and learning.
Age-dependent thrombin generation predicts 30-day mortality and symptomatic thromboembolism after multiple trauma
Authors: Lesbo Maj , Hviid Claus V. B. , Brink Ole , Juul Svend , Borris Lars C. , Hvas Anne-Mette
Published: January 2023

Trauma-induced coagulopathy (TIC) is a risk factor for death and is associated with deviations in thrombin generation. TIC prevalence and thrombin levels increase with age. We assayed in vivo and ex vivo thrombin generation in injured patients (n = 418) to specifically investigate how age impacts thrombin generation in trauma and to address the prognostic ability of thrombin generation. Biomarkers of thrombin generation were elevated in young (< 40 years) and older (≥ 40 years) trauma patients. In vivo thrombin generation was associated with Injury Severity Score (ISS) and this association was stronger in young than older patients. In vivo thrombin generation decreased faster after trauma in the young than the older patients. Across age groups, in vivo thrombin generation separated patients dying/surviving within 30 days at a level comparable to the ISS score (AUC 0.80 vs. 0.82, p > 0.76). In vivo and ex vivo thrombin generation also predicted development of thromboembolic events within the first 30 days after the trauma (AUC 0.70–0.84). In conclusion, younger trauma patients mount a stronger and more dynamic in vivo thrombin response than older patients. Across age groups, in vivo thrombin generation has a strong ability to predict death and/or thromboembolic events 30 days after injury.
Annual changes in grip strength and skeletal muscle mass in chronic liver disease: observational study
Authors: Endo Kei , Kakisaka Keisuke , Kuroda Hidekatsu , Miyasaka Akio , Takikawa Yasuhiro , Matsumoto Takayuki
Published: January 2023

Sarcopenia is a common complication in patients with chronic liver disease (CLD); however, the progression of sarcopenia over the course of CLD is unclear. The present study therefore determined the natural course of the progression of sarcopenia in patients with CLD and the effect of liver cirrhosis (LC) on this progression. This observational study analyzed patients with chronic hepatitis (CH) (n = 536) and LC (n = 320) who underwent evaluations of the grip strength and skeletal muscle mass of the arms, trunk, and legs for sarcopenia between 2016 and 2021. A bioelectrical impedance analysis was used to evaluate skeletal muscle mass. The annual rate of change (%/year) in two tests were compared between patients with CH and LC. The annual rates of change in grip strength and skeletal muscle of arms, trunk, and legs of patients with CH and LC were − 0.84% vs. − 2.93%, − 0.54% vs. − 1.71%, − 0.43% vs. − 1.02%, and − 0.76% vs. − 1.70% for men and − 0.12% vs. − 1.71%, − 0.66% vs. − 1.71%, − 0.49% vs. − 1.31%, and − 0.76% vs. − 1.54% for women, respectively. The progression of sarcopenia was greater in LC patients than in CH patients and that the decrease in grip strength was most prominent in the progression of sarcopenia in patients with LC.
Gamma irradiation mediated production improvement of some myco-fabricated nanoparticles and exploring their wound healing, anti-inflammatory and acetylcholinesterase inhibitory potentials
Authors: El-Sayed El-Sayed R. , Mansour Doaa S. , Morsi Reham M. , Elmonem Hanan A. Abd
Published: January 2023

In the current scenario, scaling up the microbial production of nanoparticles with diverse biological applications is an emerging prospect for NPs’ sustainable industry. Thus, this paper was conducted to develop a suitable applicative process for the myco-fabrication of cobalt-ferrite (CoFeNPs), selenium (SeNPs), and zinc oxide (ZnONPs) nanoparticles. A strain improvement program using gamma irradiation mutagenesis was applied to improve the NPs-producing ability of the fungal strains. The achieved yields of CoFeNPs, SeNPs, and ZnONPs were intensified by a 14.47, 7.85, and 22.25-fold increase from the initial yield following gamma irradiation and isolation of stable mutant strains. The myco-fabricated CoFeNPs, SeNPs, and ZnONPs were then exploited to study their wound healing, and anti-inflammatory. In addition, the acetylcholinesterase inhibition activities of the myco-fabricated NPs were evaluated and analyzed by molecular docking. The obtained results confirmed the promising wound healing, anti-inflammatory, and acetylcholinesterase inhibition potentials of the three types of NPs. Additionally, data from analyzing the interaction of NPs with acetylcholinesterase enzyme by molecular docking were in conformation with the experimental data.
Truncated ring-A amaryllidaceae alkaloid modulates the host cell integrated stress response, exhibiting antiviral activity to HSV-1 and SARSCoV-2
Authors: McNulty James , Babu-Dokuburra Chanti , Scattolon Jon , Zepeda-Velazquez Carlos , Wesesky Maribeth A. , Caldwell Jill K. , Zheng Wenxiao , Milosevic Jadranka , Kinchington Paul R. , Bloom David C. , Nimgaonkar Vishwajit L. , D’Aiuto Leonardo
Published: January 2023

The total synthesis of four novel mono-methoxy and hydroxyl substituted ring-A dihydronarciclasine derivatives enabled identification of the 7-hydroxyl derivative as a potent and selective antiviral agent targeting SARSCoV-2 and HSV-1. The concentration of this small molecule that inhibited HSV-1 infection by 50% (IC50), determined by using induced pluripotent stem cells (iPCS)-derived brain organ organoids generated from two iPCS lines, was estimated to be 0.504 µM and 0.209 µM. No significant reduction in organoid viability was observed at concentrations up to 50 mM. Genomic expression analyses revealed a significant effect on host-cell innate immunity, revealing activation of the integrated stress response via PERK kinase upregulation, phosphorylation of eukaryotic initiation factor 2α (eIF2α) and type I IFN, as factors potentiating multiple host-defense mechanisms against viral infection. Following infection of mouse eyes with HSV-1, treatment with the compound dramatically reduced HSV-1 shedding in vivo.
Experimental evaluation of the energy dissipation efficiency of the vortex flow section of drop shafts
Authors: Mahmoudi-Rad Mohammad , Najafzadeh Mohammad
Published: January 2023

In urban wastewater collection and drainage networks, vortex structures are recruited to transfer fluid between two conduits with significant level differences. During the drop shaft, in addition to preventing the fluid from falling due to vortex flow formation, a significant amount of the fluid energy is dissipated due to wall friction of vertical shaft. In the present study, by constructing a physical model with a scale of 1:10 made of Plexiglas, the energy dissipation efficiency in the vertical shaft has been investigated. In this way, the performance of dimensional analysis indicates that the flow Froude number (Fr) and the ratio of drop total height to shaft diameter (L⁄D) are parameters affecting the efficiency of flow energy dissipation in the vertical shaft (ηs). This research considers four levels of Fr factor (1.77, 2.01, 2.18, and 2.32) and three levels of L⁄D factor (10, 13, and 16). Additionally, four replications for 12 possible combinations allow us to carry out 48 experiments and the full factorial method. The results demonstrated that the energy dissipation efficiency in the vertical shaft changes varies from 10.80 to 62.29%. Moreover, ηs values decrease with an increase in Fr whereas the efficiency increases with increasing L⁄D ratio. Furthermore, the regression analysis gave a second-order polynomial equation which is a function of Fr and L⁄D to accurately estimate the flow energy dissipation efficiency in the vertical shaft.
Enhanced intelligent approach for determination of crude oil viscosity at reservoir conditions
Authors: Peiro Ahmady Langeroudy Kiana , Kharazi Esfahani Parsa , Khorsand Movaghar Mohammad Reza
Published: January 2023

Oil viscosity plays a prominent role in all areas of petroleum engineering, such as simulating reservoirs, predicting production rate, evaluating oil well performance, and even planning for thermal enhanced oil recovery (EOR) that involves fluid flow calculations. Experimental methods of determining oil viscosity, such as the rotational viscometer, are more accurate than other methods. The compositional method can also properly estimate oil viscosity. However, the composition of oil should be determined experimentally, which is costly and time-consuming. Therefore, the occasional inaccessibility of experimental data may make it inevitable to look for convenient methods for fast and accurate prediction of oil viscosity. Hence, in this study, the error in viscosity prediction has been minimized by taking into account the amount of dissolved gas in oil (solution gas–oil ratio: Rs) as a representative of oil composition along with other conventional black oil features including temperature, pressure, and API gravity by employing recently developed machine learning methods based on the gradient boosting decision tree (GBDT): extreme gradient boosting (XGBoost), CatBoost, and GradientBoosting. Moreover, the advantage of the proposed method lies in its independence to input viscosity data in each pressure region/stage. The results were then compared with well-known correlations and machine-learning methods employing the black oil approach applying least square support vector machine (LSSVM) and compositional approach implementing decision trees (DTs). XGBoost is offered as the best method with its greater precision and lower error. It provides an overall average absolute relative deviation (AARD) of 1.968% which has reduced the error of the compositional method by half and the black oil method (saturated region) by five times. This shows the proper viscosity prediction and corroborates the applied method's performance.
Repellent efficacy of 20 essential oils on Aedes aegypti mosquitoes and Ixodes scapularis ticks in contact-repellency assays
Authors: Luker Hailey A. , Salas Keyla R. , Esmaeili Delaram , Holguin F. Omar , Bendzus-Mendoza Harley , Hansen Immo A.
Published: January 2023

Cases of mosquito- and tick-borne diseases are rising worldwide. Repellent products can protect individual users from being infected by such diseases. In a previous study, we identified five essential oils that display long-distance mosquito repellency using a Y-tube olfactometer assay. In the current study, the contact repellent efficacy of 20 active ingredients from the Environmental Protection Agency’s (EPA) Minimum Risk Pesticides list were tested using Aedes aegypti and Ixodes scapularis. We utilized an arm-in-cage assay to measure complete protection time from mosquito bites for these active ingredients. To measure tick repellency, we used an EPA-recommended procedure to measure the complete protection time from tick crossings. We found that of the 20 ingredients tested, 10% v/v lotion emulsions with clove oil or cinnamon oil provided the longest protection from both mosquito bites and tick crossings. We conclude that in a 10% v/v emulsion, specific active ingredients from the EPA Minimum Risk Pesticides list can provide complete protection from mosquito bites and tick crossings for longer than one hour.
Analysis of dog breed diversity using a composite selection index
Authors: Hsu Wei-Tse , Williamson Peter , Khatkar Mehar Singh
Published: January 2023

During breed development, domestic dogs have undergone genetic bottlenecks and sustained selective pressures, as a result distinctive genomic diversity occurs to varying degrees within and between breed groups. This diversity can be identified using standard methods or combinations of these methods. This study explored the application of a combined selection index, composite selection signals (CSS), derived from multiple methods to an existing genotype dataset from three breed groups developed in distinct regions of Asia: Qinghai-Tibet plateau dogs (adapted to living at altitude), Xi dogs (with superior running ability) and Mountain hounds (used for hunting ability). The CSS analysis confirmed top ranked genomic regions on CFA10 and CFA21 in Qinghai-Tibet plateau dogs, CFA1 in Xi dogs and CFA5 in Mountain hounds. CSS analysis identified additional significant genomic regions in each group, defined by a total of 1,397, 1,475 and 1,675 significant SNPs in the Qinghai-Tibetan Plateau dogs, Xi dogs and Mountain hounds, respectively. Chitinase 3 Like 1 (CHI3L1) and Leucine Rich Repeat Containing G Protein-Coupled Receptor 6 (LGR6) genes were located in the top ranked region on CFA7 (0.02–1 Mb) in the Qinghai-Tibetan Plateau dogs. Both genes have been associated with hypoxia responses or altitude adaptation in humans. For the Xi dogs, the top ranked region on CFA25 contained the Transient Receptor Potential Cation Channel Subfamily C Member 4 (TRPC4) gene. This calcium channel is important for optimal muscle performance during exercise. The outstanding signals in the Mountain dogs were on CFA5 with 213 significant SNPs that spanned genes involved in cardiac development, sight and generation of biochemical energy. These findings support the use of the combined index approach for identifying novel regions of genome diversity in dogs. As with other methods, the results do not prove causal links between these regions and phenotypes, but they may assist in focusing future studies that seek to identify functional pathways that contribute to breed diversity.
Narrowing region for tropical convections in the western North Pacific
Authors: Yun Sanghyeon , Kang Namyoung , Jang Chan Joo
Published: January 2023

Considering that the subtropical highs and tropical convections are observed as negative and positive vorticities respectively, the large-scale features of the atmospheric environment can be effectively represented using streamfunctions as defined by the Laplacian. By investigating the geographical patterns of streamfunctions from different modes of environmental variability, this study conceptualizes how the subtropical high expands and the region for tropical convections migrates in the western North Pacific. It is confirmed that, owing to the expansion of the subtropical high, the limited ocean area for tropical convections even bounded by the equator becomes narrower in the “La Niña mode” than that in the “El Niño mode”. This study finds that a warmer environment is likely to further expand the subtropical high to the west, and then the westernmost shift in the region for tropical convections appears in the “warmer La Niña mode”. A linear perspective suggests that every warmer La Niña environment could be one that people have scarcely experienced before.
Characterization of graphene oxide-ziziphus seeds and its application as a hazardous dye removal adsorbent
Authors: Al-Shehri Badria M. , Al-Zahrani Fatimah A. M. , El-Shishtawy Reda M. , Awwad Nasser S. , Sayed M. A. , Khan Khalid Ali
Published: January 2023

The zizphus seeds are considered as a biomaterial residues that has been used for removing of organic industrial waste such as 2-((10-octyl-9,10-dihydroanthracene-2-yl) methylene) malononitrile (PTZS-CN) dye from aqueous solutions utilizing graphene oxide-Ziziphus (GO-Ziziphus). A batch study explored the impacts of various experimental circumstances, including solution pH, initial dye concentration, temperature, and contact time. General order, nonlinear pseudo-first order and pseudo-second order, elvoich model and intraparticiple diffusion were utilized to analyze the kinetic data. The adsorption kinetics of dye onto GO-ziziphus adsorption was best mentioned by nonlinear pseudo-first order. Similarly, the intra-particle diffusion plots revealed one exponential line throughout the adsorption process. The Freundlich, Dubinin-Radushkevich, and Langmuir models were employed to examine isothermal data. It provided the best fit of the dye adsorption isothermal data onto GO-ziziphus Freundlich models. Besides, the calculated free energies showed that the adsorption progression was physical adsorption. Thermodynamic calculations revealed that dye adsorption onto GO-ziziphus was exothermic and spontaneous. The combined results indicated that GO-ziziphus powder might be used to treat dye-rich wastewater effectively.
Research on stress performance of universally wrapped assembled joints of lattice wind power tower with concrete-filled steel tube
Authors: Wen Yang , Liu Zhen , Xiong Wen
Published: January 2023

With the control parameters of the wall thickness of the ball table and the thickness of the pressure plate of the ball table, four universal wrapped assembled joint models were designed for the research of stress performance via static loading test. The failure mode of the joint, the axial force–displacement curve of the web member, the equivalent stress distribution of the ball table and the pressure plate of the ball table were analyzed, and the ABQAUS finite element software was used for the analysis of parameter expansion of the specimen. The research results show that the failure modes of universally wrapped assembled joints can be divided into the buckling failure of the ball table and the strength failure of the material. The wall thickness of the ball table is the key parameter affecting the failure mode of the joints, and the thickness of the pressure plate of the ball table is the crucial parameter influencing the ultimate bearing capacity of the joints. The maximum equivalent stress of the pressure plate and the ball table at compression zone for all the joints is occurred at the side squeezed by the bolt ball, and the growth rate of the equivalent stress for the ball table at compression zone is more uniform than that at tension zone. According to the finite element analysis results, when the wall thickness of the ball table is greater than 7 mm, the thickness of the pressure plate of the ball table is greater than 16 mm, the growth rate of the ultimate bearing capacity of the joint is significantly decreased, therefore, it is recommended to use the wall thickness of 7 mm and the pressure plate thickness of 16 mm in the actual design of the project.
Coral reef structural complexity loss exposes coastlines to waves
Authors: Carlot Jérémy , Vousdoukas Michalis , Rovere Alessio , Karambas Theofanis , Lenihan Hunter S. , Kayal Mohsen , Adjeroud Mehdi , Pérez-Rosales Gonzalo , Hedouin Laetitia , Parravicini Valeriano
Published: January 2023

Coral reefs offer natural coastal protection by attenuating incoming waves. Here we combine unique coral disturbance-recovery observations with hydrodynamic models to quantify how structural complexity dissipates incoming wave energy. We find that if the structural complexity of healthy coral reefs conditions is halved, extreme wave run-up heights that occur once in a 100-years will become 50 times more frequent, threatening reef-backed coastal communities with increased waves, erosion, and flooding.
BORC complex specific components and Kinesin-1 mediate autophagy evasion by the autophagy-resistant Mycobacterium tuberculosis Beijing strain
Authors: Tunganuntarat Janpen , Kanjanasirirat Phongthon , Khumpanied Tanawadee , Benjaskulluecha Salisa , Wongprom Benjawan , Palaga Tanapat , Siregar Tegar Adriansyah Putra , Borwornpinyo Suparerk , Chaiprasert Angkana , Palittapongarnpim Prasit , Ponpuak Marisa
Published: January 2023

Autophagy induction by starvation has been shown to enhance lysosomal delivery to mycobacterial phagosomes, resulting in the restriction of the Mycobacterium tuberculosis reference strain H37Rv. In contrast to H37Rv, our previous study showed that strains belonging to the notorious M. tuberculosis Beijing genotype could evade autophagic elimination. Our recent RNA-Seq analysis also discovered that the autophagy-resistant M. tuberculosis Beijing strain (BJN) evaded autophagic control by upregulating the expression of Kxd1, a BORC complex component, and Plekhm2, both of which function in lysosome positioning towards the cell periphery in host macrophages, thereby suppressing enhanced lysosomal delivery to its phagosome and sparing the BJN from elimination as a result. In this work, we further characterised the other specific components of the BORC complex, BORC5-8, and Kinesin proteins in autophagy resistance by the BJN. Depletion of BORCS5-8 and Kinesin-1, but not Kinesin-3, reverted autophagy avoidance by the BJN, resulting in increased lysosomal delivery to the BJN phagosomes. In addition, the augmented lysosome relocation towards the perinuclear region could now be observed in the BJN-infected host cells depleted in BORCS5-8 and Kinesin-1 expressions. Taken together, the data uncovered new roles for BORCS5-8 and Kinesin-1 in autophagy evasion by the BJN.
Epithelial cell-derived cytokine TSLP activates regulatory T cells by enhancing fatty acid uptake
Authors: Kasuya Tadamichi , Tanaka Shigeru , Tamura Jun , Etori Keishi , Shoda Jumpei , Hattori Koto , Endo Yusuke , Kitajima Masayuki , Kageyama Takahiro , Iwamoto Taro , Yokota Masaya , Iwata Arifumi , Suto Akira , Suzuki Kotaro , Suzuki Harumi , Ziegler Steven F. , Nakajima Hiroshi
Published: January 2023

Epithelial cells control a variety of immune cells by secreting cytokines to maintain tissue homeostasis on mucosal surfaces. Regulatory T (Treg) cells are essential for immune homeostasis and for preventing tissue inflammation; however, the precise molecular mechanisms by which epithelial cell-derived cytokines function on Treg cells in the epithelial tissues are not well understood. Here, we show that peripheral Treg cells preferentially respond to thymic stromal lymphoprotein (TSLP). Although TSLP does not affect thymic Treg differentiation, TSLP receptor-deficient induced Treg cells derived from naïve CD4+ T cells are less activated in an adoptive transfer model of colitis. Mechanistically, TSLP activates induced Treg cells partially through mTORC1 activation and fatty acid uptake. Thus, TSLP modulates the activation status of induced Treg through the enhanced uptake of fatty acids to maintain homeostasis in the large intestine.