bluuebunny/crossref_metadata_2025 huggingface dataset:
Deciphering the origin and therapeutic targets of cancer of unknown primary: a case report that illustrates the power of integrative whole-exome and transcriptome sequencing analysis
Authors: Al Assaad Majd , Shin Nara , Sigouros Michael , Manohar Jyothi , Antysheva Zoia , Kotlov Nikita , Kiriy Daria , Nikitina Anastasiia , Kleimenov Mikhail , Tsareva Anastasiya , Makarova Anastasiya , Fomchenkova Victoria , Dubinina Julia , Boyko Alexandra , Almog Nava , Wilkes David , Escalon Joanna G. , Saxena Ashish , Elemento Olivier , Sternberg Cora N. , Nanus David M. , Mosquera Juan Miguel
Published: January 2024

Cancer of unknown primary (CUP) represents a significant diagnostic and therapeutic challenge, being the third to fourth leading cause of cancer death, despite advances in diagnostic tools. This article presents a successful approach using a novel genomic analysis in the evaluation and treatment of a CUP patient, leveraging whole-exome sequencing (WES) and RNA sequencing (RNA-seq). The patient, with a history of multiple primary tumors including urothelial cancer, exhibited a history of rapid progression on empirical chemotherapy. The application of our approach identified a molecular target, characterized the tumor expression profile and the tumor microenvironment, and analyzed the origin of the tumor, leading to a tailored treatment. This resulted in a substantial radiological response across all metastatic sites and the predicted primary site of the tumor. We argue that a comprehensive genomic and molecular profiling approach, like the BostonGene© Tumor Portrait, can provide a more definitive, personalized treatment strategy, overcoming the limitations of current predictive assays. This approach offers a potential solution to an unmet clinical need for a standardized approach in identifying the tumor origin for the effective management of CUP.
Efficacy and pharmacoeconomic advantages of Fufang Huangbai Fluid hydropathic compress in diabetic foot infections: a comparative clinical study with antimicrobial calcium alginate wound dressing
Authors: Yang Guangyao , Wang Gang , Li Zhenghong , Deng Lijuan , Wang Ning , Wang Xuewan , Zhou Tong , Zhang Jingming , Lei Yin , Wang Tao , Wang Yue , Shao Hanying , Chen Mingya , Zhang Keren , Zhou Min , Wang Xiangbao , Liu Xingfang , Ju Shang
Published: January 2024

Objective: To compare the intervention effects and pharmacoeconomic advantages of Fufang Huangbai Fluid (FFHB) hydropathic compress versus Antimicrobial Calcium Alginate Wound Dressing (ACAWD) in the treatment of diabetic foot infections (DFI).

Methods: Patients with DF who were hospitalized in the peripheral vascular Department of Dongzhimen Hospital of Beijing University of Chinese Medicine from December 2020 to February 2022 and met the inclusion and excluding criteria were allocated into the experimental group and control group through minimization randomization. The experimental group was treated with FFHB hydropathic compress for 2 weeks, while the control group was treated with ACAWD for the same duration. The wound healing of both groups was monitored for 1 month post-discharge. Clinical data from all eligible patients were collected, and differences in various indices between cohorts were analyzed.

Results: 22 in the experimental group (including two fell off) and 20 in the control group. After the treatment, the negative rate of wound culture in the experimental group was 30% and that in the control group was 10%, There was no significant difference in the negative rate of wound culture and change trend of minimum inhibitory concentration (MIC) value of drug sensitivity (p > 0.05). The infection control rate of the experimental group was 60%, and that of the control group was 25%. The difference between the two groups was statistically significant (χ2 = 5.013, p = 0.025). The median wound healing rate of the experimental group was 34.4% and that of the control group was 33.3%. There was no significant difference between the two groups (p > 0.05). During the follow-up 1 month later, the wound healing rate in the experimental group was higher, and the difference was statistically significant (p = 0.047). Pharmacoeconomic evaluations indicated that the experimental group had greater cost-effectiveness compared to the control group.

Conclusion: In the preliminary study, FFHB demonstrated comparable pathogenic and clinical efficacy to ACAWD in the treatment of mild DF infection, and exhibited superior pharmacoeconomic advantages. With the aid of infection control, the wound healing rate in the FFHB group showed notable improvement. Nevertheless, due to the limited sample size, larger-scale studies are warranted to further validate these findings.

Clinical Trial Registration: (https://www.chictr.org.cn/showproj.aspx?proj=66175), identifier (ChiCTR2000041443).
Economic evaluation of management strategies for complex regional pain syndrome (CRPS)
Authors: Zhou Xiaofeng , Zhou Yi , Zhang Xiaofei , Jiang Feng
Published: January 2024

Background:The economic impact of Complex Regional Pain Syndrome (CRPS) on both patients and the global healthcare system continues to escalate. However, the economic implications associated with management interventions for CRPS have received limited attention. Therefore, our objective is to perform a thorough examination of published economic assessments of the various management strategies utilized for CRPS.

Methods:A thorough search spanning four general medical databases and three health economic databases to identify full economic evaluations on CRPS management strategies from January 1994 to June 2023 were conducted. The quality of these studies were evaluated by employing the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) statement. To enable cross-study comparisons conducted in different countries, we adjusted the costs reported in the selected studies for inflation and converted them into 2023 US dollars.

Results:A total of nine economic evaluations, consisting of eight high-quality and one medium-quality, were identified across five nations during a span of 29 years. The findings suggest that the most economically efficient intervention for CRPS are interventional approaches of Spinal Cord Stimulation (SCS) in comparison to conventional management for long periods of time. Furthermore, in situations where there is a limited time period of less than 1 year, rehabilitation therapies, particularly physical therapy, have been demonstrated to be more effective in terms of both cost and clinical outcomes.

Conclusion:The interventional management strategies, particularly for severe and persistent CRPS over long periods, may offer the greatest cost efficiency. In conditions with limited timelines, rehabilitation measures, such as rehabilitation therapies, can be cost-effective. However, insufficient data for other common interventions prevents the formation of a definitive conclusion. Similarly, it is crucial to recognize that the results of these interventions might be affected by the selection of comparator and the threshold for willingness to pay.
Sociodemographic and clinical predictors of adherence to antidepressants in depressive disorders: a systematic review with a meta-analysis
Authors: Del Pino-Sedeño Tasmania , Infante-Ventura Diego , Hernández-González Diego , González-Hernández Yadira , González de León Beatriz , Rivero-Santana Amado , Hurtado Isabel , Acosta Artiles Francisco Javier
Published: January 2024

Introduction: Current evidence reveals concerning rates of non-adherence to antidepressant treatment, possibly influenced by various relevant determinants such as sociodemographic factors or those related to the health system and their professionals. The aim of this paper is to review the scientific evidence on sociodemographic and clinical predictors of adherence to pharmacological treatment in patients diagnosed with a depressive disorder.

Methods: a systematic review (SR) was conducted. The search for a previous SR was updated and de novo searches were performed in Medline, EMBASE, Web of Science (WoS) and PsycInfo (last 10 years). The risk of bias was assessed using the Cochrane tool for non-randomized studies—of Exposure (ROBINS-E). Meta-analyses were conducted.

Results: Thirty-nine studies (n = 2,778,313) were included, 24 of them in the meta-analyses. In the initiation phase, no association of adherence was found with any of the predictors studied. In the implementation and discontinuation phases, middle-aged and older patients had better adherence rates and lower discontinuation rates than younger ones. White patients adhered to treatment better than African-American patients.

Discussion: Age and ethnicity are presented as the predictive factors of pharmacological adherence. However, more research is needed in this field to obtain more conclusive results on other possible factors.

Systematic Review Registration: [https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023414059], identifier [CRD42023414059]
Screening and evaluation of metabolites binding PRAS40 from Erxian decoction used to treat spinal cord injury
Authors: Lin Li , Yan Jingchuan , Sun Jin , Zhang Jianfeng , Liao Bo
Published: January 2024

Objective: The PRAS40 is an essential inhibitory subunit of the mTORC1 complex, which regulates autophagy. It has been suggested that Erxian Decoction (EXD) could treat spinal cord injury (SCI) via the autophagy pathway. However, the mechanism of whether EXD acts through PRAS40 remains unclear.

Methods: With the help of immobilized PRAS40, isothermal titration calorimetry (ITC) and molecular docking, the bioactive metabolites in the EXD were screened. To establish in vitro SCI models, PC12 cells were exposed to hydrogen peroxide (H2O2) and then treated with the identified EXD substances. Furthermore, Western blot assay was carried out to identify potential molecular mechanisms involved. For assessing the effect of metabolites in vivo, the SCI model rats were first pretreated with or without the metabolite and then subjected to the immunohistochemistry (IHC) staining, Basso, Beattie & Bresnahan (BBB) locomotor rating scale, and H&E staining.

Results: The immobilized PRAS40 isolated indole, 4-nitrophenol, terephthalic acid, palmatine, sinapinaldehyde, and 3-chloroaniline as the potential ligands binding to PRAS40. Furthermore, the association constants of palmatine and indole as 2.84 × 106 M-1 and 3.82 × 105 M-1 were elucidated via ITC due to the drug-like properties of these two metabolites. Molecular docking results also further demonstrated the mechanism of palmatine binding to PRAS40. Western blot analysis of PC12 cells demonstrated that palmatine inhibited the expression of p-mTOR by binding to PRAS40, activating the autophagic flux by markedly increasing LC3. The injection of palmatine (10μM and 20 μM) indicated notably increased BBB scores in the SCI rat model. Additionally, a dose-dependent increase in LC3 was observed by IHC staining.

Conclusion: This research proved that EXD comprises PRAS40 antagonists, and the identified metabolite, palmatine, could potentially treat SCI by activating the autophagic flux.
Phenotyping of light-activated neurons in the mouse SCN based on the expression of FOS and EGR1
Authors: Riedel Casper Schwartz , Georg Birgitte , Hannibal Jens
Published: January 2024

Light-sensitive neurons are located in the ventral and central core of the suprachiasmatic nucleus (SCN), whereas stably oscillating clock neurons are found mainly in the dorsal shell. Signals between the SCN core and shell are believed to play an important role in light entrainment. Core neurons express vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP), and Neuroglobin (Ngb), whereas the shell neurons express vasopressin (AVP), prokineticin 2, and the VIP type 2 (VPAC2) receptor. In rodents, light has a phase-shifting capacity at night, which induces rapid and transient expression of the EGR1 and FOS in the SCN.

Methods: The present study used immunohistochemical staining of FOS, EGR1, and phenotypical markers of SCN neurons (VIP, AVP, Ngb) to identify subtypes/populations of light-responsive neurons at early night.

Results: Double immunohistochemistry and cell counting were used to evaluate the number of SCN neurons expressing FOS and EGR1 in the SCN. The number of neurons expressing either EGR1 or FOS was higher than the total number of neurons co-storing EGR1 and FOS. Of the total number of light-responsive cells, 42% expressed only EGR1, 43% expressed only FOS, and 15% expressed both EGR1 and FOS. Light-responsive VIP neurons represented only 31% of all VIP neurons, and EGR1 represents the largest group of light-responsive VIP neurons (18%). VIP neurons expressing only FOS represented 1% of the total light-responsive VIP neurons. 81% of the Ngb neurons in the mouse SCN were light-responsive, and of these neurons expressing only EGR1 after light stimulation represented 44%, whereas 24% expressed FOS. Although most light-responsive neurons are found in the core of the SCN, 29% of the AVP neurons in the shell were light-responsive, of which 8% expressed EGR1, 10% expressed FOS, and 11% co-expressed both EGR1 and FOS after light stimulation.

Discussion: Our analysis revealed cell-specific differences in light responsiveness between different peptidergic and Ngb-expressing neurons in different compartments of the mouse SCN, indicating that light activates diverse neuronal networks in the SCN, some of which participate in photoentrainment.
The deterioration of starch physiochemical and minerals in high-quality indica rice under low-temperature stress during grain filling
Authors: Yang Juan , Zhang Xinzheng , Wang De , Wu Jinshui , Xu Hang , Xiao Yang , Xie Hongjun , Shi Wanju
Published: January 2024

Low temperatures during the grain-filling phase have a detrimental effect on both the yield and quality of rice grains. However, the specific repercussions of low temperatures during this critical growth stage on grain quality and mineral nutrient composition in high-quality hybrid indica rice varieties have remained largely unexplored. The present study address this knowledge gap by subjecting eight high-quality indica rice varieties to two distinct temperature regimes: low temperature (19°C/15°C, day/night) and control temperature (28°C/22°C) during their grain-filling phase, and a comprehensive analysis of various quality traits, with a particular focus on mineral nutrients and their interrelationships were explored. Exposure of rice plants to low temperatures during early grain filling significantly impacts the physicochemical and nutritional properties. Specifically, low temperature increases the chalkiness rate and chalkiness degree, while decreases starch and amylopectin content, with varying effects on amylose, protein, and gelatinization temperature among rice varieties. Furthermore, crucial parameters like gelatinization enthalpy (ΔH), gelatinization temperature range (R), and peak height index (PHI) all significantly declined in response to low temperature. These detrimental effects extend to rice flour pasting properties, resulting in reduced breakdown, peak, trough, and final viscosities, along with increased setback. Notably, low temperature also had a significant impact on the mineral nutrient contents of brown rice, although the extent of this impact varied among different elements and rice varieties. A positive correlation is observed between brown rice mineral nutrient content and factors such as chalkiness, gelatinization temperature, peak viscosity, and breakdown, while a negative correlation is established with amylose content and setback. Moreover, positive correlations emerge among the mineral nutrient contents themselves, and these relationships are further accentuated in the context of low-temperature conditions. Therefore, enhancing mineral nutrient content and increasing rice plant resistance to chilling stress should be the focus of breeding efforts to improve rice quality.
Comprehensive phytochemical profiles and antioxidant activity of Korean local cultivars of red chili pepper (Capsicum annuum L.)
Authors: Jang Hyemi , Choi Mira , Jang Kyoung-Soon
Published: January 2024

Red chili pepper (Capsicum annuum L.), which belongs to the Solanaceae family, contains a variety of phytochemicals with health-promoting properties including capsaicinoids, phenolics and fatty acids. Red chili pepper is one of the most consumed vegetables in Korea and occupies the largest cultivated area among spices. In this study, the ethanolic extracts from two Korean local cultivars, namely Subicho and Eumseong, were analyzed using a hybrid trapped ion mobility Q-TOF mass spectrometer equipped with a UPLC system, and their phytochemical profiles were then compared with those of a common phytophthora disease-resistant cultivar called Dokbulwang, which is extensively used for red chili pepper powder in public spaces across Korea. Utilizing high-resolution ion-mobility Q-TOF MS analysis, 458 and 192 compounds were identified from the three different red chili peppers in positive and negative ion modes, respectively, by matching with a reference spectral library. Principal component analysis revealed clear distinctions among the three cultivars, allowing us to identify key phytochemical components responsible for discriminating the local cultivars from the public cultivar. Furthermore, the assessment of total flavonoid, phenolic, and antioxidant activity in the red pepper extracts, highlighted their diverse molecular and chemical profiles. Despite the higher total flavonoid and phenolic content values observed in the public cultivar, the radical scavenging rate was higher in the local cultivars, particularly in Subicho. This suggest the presence of stronger antioxidant compounds in the local cultivar, indicating their potential health benefits due to their rich content of bioactive compounds. Notably, the local cultivars exhibited significantly higher proportions of organic compounds (more than four times) and terpenoids (more than two times) compared to the public cultivar. Specifically, higher levels of five major capsaicinoid compounds were found in the local cultivars when compared to the public cultivar. The observed disparities in phytochemical composition and antioxidant activities indicate the molecular diversity present among these cultivars. Further exploration of the bioactive compounds in these local cultivars could prove invaluable for the development of native crops, potentially leading to the discovery of novel sources of bioactive molecules for various applications in health and agriculture.
Enhancing EEG-based attachment style prediction: unveiling the impact of feature domains
Authors: Laufer Ilan , Mizrahi Dor , Zuckerman Inon
Published: January 2024

Introduction

Attachment styles are crucial in human relationships and have been explored through neurophysiological responses and EEG data analysis. This study investigates the potential of EEG data in predicting and differentiating secure and insecure attachment styles, contributing to the understanding of the neural basis of interpersonal dynamics.

Methods

We engaged 27 participants in our study, employing an XGBoost classifier to analyze EEG data across various feature domains, including time-domain, complexity-based, and frequency-based attributes.

Results

The study found significant differences in the precision of attachment style prediction: a high precision rate of 96.18% for predicting insecure attachment, and a lower precision of 55.34% for secure attachment. Balanced accuracy metrics indicated an overall model accuracy of approximately 84.14%, taking into account dataset imbalances.

Discussion

These results highlight the challenges in using EEG patterns for attachment style prediction due to the complex nature of attachment insecurities. Individuals with heightened perceived insecurity predominantly aligned with the insecure attachment category, suggesting a link to their increased emotional reactivity and sensitivity to social cues. The study underscores the importance of time-domain features in prediction accuracy, followed by complexity-based features, while noting the lesser impact of frequency-based features. Our findings advance the understanding of the neural correlates of attachment and pave the way for future research, including expanding demographic diversity and integrating multimodal data to refine predictive models.
Isolating unique variance in mental health outcomes attributable to personality variables and childhood emotional abuse
Authors: Van Landeghem Chantal , Jakobson Lorna S.
Published: January 2024

Introduction

University students are at high risk for anxiety and depression. Our main objective was to tease apart variance in symptom severity that was uniquely attributable to four associated variables that are frequently confounded: exposure to childhood emotional abuse, alexithymia, sensory processing sensitivity (SPS), and anxiety sensitivity (AS).

Methods

University students (N = 410) completed an online survey designed to measure our four key study variables along with several other potentially relevant variables including sex, physical activity levels, and perceived COVID-19 impacts.

Results

Over half of the participants reported moderate to extremely severe symptoms of anxiety and depression. Females reported stronger signs of SPS and AS and were more likely than males to have increased their moderate/vigorous exercise since the pandemic began. After controlling for the other variables, the best predictors of perceived COVID-19 impacts were SPS, childhood emotional abuse, and current levels of physical activity. Whereas all three personality variables and childhood emotional abuse emerged as significant predictors of both depression and anxiety, neither COVID-19 impacts nor physical activity levels accounted for unique variance in either model. Unexpectedly, male sex emerged as an additional risk factor for depression, raising the possibility that males experience unique stressors and societal pressures that increase their risk of depression.

Discussion

These findings help to clarify the links between childhood emotional abuse, personality traits implicated in emotional awareness and self-regulation, and mental health. They may have important implications for the development and implementation of individualized treatments for common mental disorders.
Digital psychosocial interventions for individuals with spinal cord injury: a scoping review
Authors: Armstrong Alice , Oetinger Katja , Weimer Katja , Hönig Klaus
Published: January 2024

Objective

To provide an overview of the digital mental health care landscape for individuals with spinal cord injury (SCI).

Methods

PubMed, PsycInfo, and PSYNDEX were searched for articles meeting the following criteria: (1) article written in English or German; (2) digital psychosocial intervention; (3) SCI only; (4) treatment of individuals with SCI and not their relatives or caregivers. Records were screened by title and abstract and records meeting the inclusion criteria were obtained for full text screening. The references of identified articles were screened to find further relevant articles. The literature search was updated before submission. Risk of Bias was assessed by using the Cochrane risk-of-bias tool for randomized trials (RoB 2) and a narrative synthesis was conducted.

Results

Ten randomized-controlled trials (RCT) and ten non-randomized-controlled trials were identified and compared in this review, evaluating twelve internet- and mobile-based interventions, five smartphone apps, and three virtual reality applications. The interventions were primarily used as stand-alone aftercare programs. While some were not based on any theory, cognitive behavioral therapy mostly served as the theoretical basis for the online interventions. The extent of human support also varied greatly between the studies. The number of intervention modules ranged between 2 and 72. There were also major differences in outcome variables and effects. A meta-analytical evaluation of the data was not conducted due to heterogeneity of studies.

Conclusion

Digital applications to promote the psychosocial health of individuals with SCI are an emerging field of research with many treatment approaches still to come. First high quality RCT studies report promising results. Unfortunately, not all studies are of high quality or the interventions have been insufficiently adapted to the needs of people with SCI. Therefore, more research is needed to further develop applications, and to generalize and test the effects found in the long term.
Incentivised physical activity intervention promoting daily steps among university employees in the workplace through a team-based competition
Authors: Safi Ayazullah , Deb Sanjoy , Kelly Adam , Cole Matthew , Walker Natalie , Zariwala Mohammed Gulrez
Published: January 2024

Introduction

The benefits of walking on health and well-being is well established and regarded as the most accessible form of physical activity (PA) that most individuals can incorporate into their lives. Despite the benefits, the impact of a competitive walking intervention combined with a prize incentive in the workplace is yet to be established. The aim of this intervention was to promote PA among university employees through teams-based competition with a prize incentive targeted towards the recommended 10,000 steps per day.

Methods

A total of 49 employees participated and formed eight departmental teams ranging from Senior Admin management, Educational & Social work, Nursing & Midwifery, Sport & Exercise, Health Sciences, Admin Assistant, Library, and IT to compete in a walking intervention. Each team was handed an ActiGraph wGT3X-BT from Monday to Friday to record their walking steps. Steps. Post intervention participants completed an open-ended survey to provide their views about the intervention.

Results

The ActiGraph findings determined that steps increased by 4,799 per day from daily baseline of 5,959 to 10,758 throughout this intervention. The themes from qualitative data showed that the prize incentive and competitive nature of this intervention has motivated staff to walk more, changed their behaviour, enjoyed the team-based competition, and improved perceived productivity in the workplace.

Discussion and conclusion

This intervention increased employees’ daily steps by 4,799 and met the 10,000 steps guideline. The ‘Health Sciences’ team recorded the highest steps 531,342 followed by the ‘Education and Social Work’ accumulating 498,045 steps throughout this intervention. This intervention with prize incentive demonstrated a positive impact on employees personal and work-based outcomes as well as contributed to the workplace PA, health, and wellbeing literature, and more specifically, to the scarce research focused on university settings.
Factors affecting work-related non-fatal injuries among aged workers in South Korea
Authors: Park Jungsun , Park Jong-shik , Jung Younghoon , Na Minoh , Kim Yangho
Published: January 2024

Introduction

The objective of this paper is to investigate whether an aging workforce is associated with an increase in work-related non-fatal injuries and to explore the underlying reasons for this potential increase.

Methods

Aged workers were defined as those who were at least 55-years-old. Work-related non-fatal injuries were assessed in aged and young workers who were registered with the workers’ compensation system from 2017 to 2021 of South Korea.

Results

The mean estimated rate of work-related non-fatal injuries of aged workers (0.88/100) was about 2.5-times higher than that of younger workers (0.35/100). Most work-related non-fatal injuries in the older adults were in individuals working in the “construction sector” (36.0%), those with “elementary occupations (unskilled workers)” (45.0%), and those with employment status of “daily worker” (44.0%). “Trip & slip” (28.7%) and “falling” (19.6%) were more frequent types of work-related non-fatal injuries in aged workers relative to young workers. The category of “buildings, structures, and surfaces” was a more frequent cause of work-related non-fatal injuries in aged workers than young workers.

Discussion

The incidence of non-fatal work-related injuries is higher among aged workers compared to their younger counterparts. The increased occurrence of aged workers participating in precarious employment and jobs, along with the greater physical vulnerability, is likely the cause of their higher rate of work-related non-fatal injuries.
Initiation phase cellular reprogramming ameliorates DNA damage in the ERCC1 mouse model of premature aging
Authors: Paine Patrick Treat , Rechsteiner Cheyenne , Morandini Francesco , Desdín-Micó Gabriela , Mrabti Calida , Parras Alberto , Haghani Amin , Brooke Robert , Horvath Steve , Seluanov Andrei , Gorbunova Vera , Ocampo Alejandro
Published: January 2024

Unlike aged somatic cells, which exhibit a decline in molecular fidelity and eventually reach a state of replicative senescence, pluripotent stem cells can indefinitely replenish themselves while retaining full homeostatic capacity. The conferment of beneficial-pluripotency related traits via in vivo partial cellular reprogramming in vivo partial reprogramming significantly extends lifespan and restores aging phenotypes in mouse models. Although the phases of cellular reprogramming are well characterized, details of the rejuvenation processes are poorly defined. To understand whether cellular reprogramming can ameliorate DNA damage, we created a reprogrammable accelerated aging mouse model with an ERCC1 mutation. Importantly, using enhanced partial reprogramming by combining small molecules with the Yamanaka factors, we observed potent reversion of DNA damage, significant upregulation of multiple DNA damage repair processes, and restoration of the epigenetic clock. In addition, we present evidence that pharmacological inhibition of ALK5 and ALK2 receptors in the TGFb pathway are able to phenocopy some benefits including epigenetic clock restoration suggesting a role in the mechanism of rejuvenation by partial reprogramming.
Benefits Trafficking: human trafficking of older adults and adults with disabilities
Authors: Thomas Anna , Strickland Heather
Published: January 2024

An emerging type of trafficking is targeting overlooked at-risk adults. Benefits Trafficking is the systematic recruitment, harboring, neglect, and financial exploitation of elder and disabled adults who receive government benefits such as Social Security, Veteran's Benefits, Medicaid, and Medicare. Traffickers often pose as kind-hearted individuals offering to provide care for at-risk adults in an in-home setting. Once recruited, at-risk adults are stripped of their government benefits, held against their will, moved from location to location, and denied basic needs such as food, clothing, and adequate shelter. While Benefits Trafficking is a basic civil rights violation issue, it is also a growing public health issue. Victims of Benefits Trafficking are often the forgotten at-risk adults who have fallen through the cracks of various mental health systems, are unhoused, and no longer have a social support system in place. This area of human trafficking is unresearched and its prevalence is largely unknown outside of the few entities working in this space. This paper focuses mainly on raising awareness of Benefits Trafficking and suggestions for future funding and research initiatives.
A framework for multi-scale intervention modeling: virtual cohorts, virtual clinical trials, and model-to-model comparisons
Authors: Michael Christian T. , Almohri Sayed Ahmad , Linderman Jennifer J. , Kirschner Denise E.
Published: January 2024

Computational models of disease progression have been constructed for a myriad of pathologies. Typically, the conceptual implementation for pathology-related in silico intervention studies has been ad hoc and similar in design to experimental studies. We introduce a multi-scale interventional design (MID) framework toward two key goals: tracking of disease dynamics from within-body to patient to population scale; and tracking impact(s) of interventions across these same spatial scales. Our MID framework prioritizes investigation of impact on individual patients within virtual pre-clinical trials, instead of replicating the design of experimental studies. We apply a MID framework to develop, organize, and analyze a cohort of virtual patients for the study of tuberculosis (TB) as an example disease. For this study, we use HostSim: our next-generation whole patient-scale computational model of individuals infected with Mycobacterium tuberculosis. HostSim captures infection within lungs by tracking multiple granulomas, together with dynamics occurring with blood and lymph node compartments, the compartments involved during pulmonary TB. We extend HostSim to include a simple drug intervention as an example of our approach and use our MID framework to quantify the impact of treatment at cellular and tissue (granuloma), patient (lungs, lymph nodes and blood), and population scales. Sensitivity analyses allow us to determine which features of virtual patients are the strongest predictors of intervention efficacy across scales. These insights allow us to identify patient-heterogeneous mechanisms that drive outcomes across scales.
Assessment of community-based intervention approaches to improve the health and welfare of working donkeys in selected areas of Sidama region, Southern Ethiopia
Authors: Yalew Aweke , Darge Daniel , Melake Berhanu Mekibib
Published: January 2024

Background

Although efforts have been made by certain non-governmental organizations, like the Donkey Sanctuary, SPANA (Society for the Protection of Animals Abroad), and Brooke Ethiopia, to change the attitudes and practices of donkey owners toward improving the health and welfare of working donkeys, their impact has not been assessed so far. A cross-sectional study was conducted to assess and compare donkeys’ health and welfare problems in community-based intervention areas versus non-intervention areas in selected districts of Sidama regional state, Southern Ethiopia.

Methods

For the animal-based welfare assessments, 200 donkeys each were selected and included from intervention and non-intervention areas. The selected donkeys were then assessed for their welfare and health status using five important parameters, namely, body condition score, presence and severity of wounds, behavior, presence and severity of lameness, and presence of other signs of illness/diseases.

Results

The prevalence and severity of lameness and wounds on donkeys managed in non-intervention areas were higher than those observed in community-based intervention areas. The prevalence of lameness in the non-intervention areas (25.5%) was over two times higher than the prevalence in the intervention areas (12%). Likewise, over 37% of the donkeys in the non-intervention areas were wounded, of which 64% were suffering moderate to severe wounds. Moreover, donkeys in the intervention areas had better body condition and were alert and friendly upon human approach. There was a statistically significant difference (p < 0.01) between the intervention and non-intervention areas in all the considered parameters, namely, the presence of lameness, wound, body condition score, demeanor, and response to approach.

Conclusion and recommendations

Based on this study’s findings, the community-based intervention approach was found to improve the health and welfare of working donkeys. Therefore, comprehensive and continuous equine health and welfare promotion through community-based intervention approaches should be designed and implemented to improve the welfare of working equines in the country.
A humoral diagnostic test outperforms cellular tests in a farm with a latent tuberculosis outbreak caused by a new Mycobacterium tuberculosis complex spoligotype that affected sheep but not goats
Authors: Juste Ramon A. , Fernández-Veiga Leire , Fuertes Miguel , Fernández-Ortiz de Murua Ignacio , Cardona Guillermo , Geijo Maria V. , Garrido Joseba M. , Sevilla Iker A.
Published: January 2024

Tuberculosis (TB) is a disease caused by members of the M. tuberculosis complex (MTC) that affects numerous species. M. caprae, a member of the complex which is close to M. bovis, is emerging and affects several different hosts that include goats, cattle, sheep, pigs, rabbits, wild boar, red deer, foxes and also humans. A new M. caprae spoligotype (SB2737) was isolated from an outbreak of sheep tuberculosis affecting a mixed sheep (323)-goat (29) farm in 2021. The index case was detected by the La Rioja slaughterhouse veterinary inspection. Tracing back to the farm of origin, both species were submitted to Comparative Intradermal Tuberculin Test (CITT) and M. bovis-specific antibody ELISA tests. A subsample was also examined by IFN-γ release assay (IGRA) and all positives were slaughtered and pathologically and microbiologically investigated. Only 1.2% of sheep and no goat were positive in the CITT, and 11.4% in the IGRA sheep subsample, while up to 36.8% were positive in two consecutive M. bovis-specific antibody ELISA tests. Goats had always tested negative in annual intradermal follow-up since 2013. Upon confirmation of the immunologically positive sheep at slaughter, all the remaining negative animals were killed and 29.2% of sheep were still found infected. This raised the final overall prevalence to 37.5%. Antibody ELISA was the most sensitive (81.4%) in vivo detection method still showing a 85.0% specificity relative to pathological and microbiological tuberculosis status. It was nearly 10 times more sensitive than skin test and had an 86.8% positive predictive value. Notwithstanding a possible singular pathogenesis of the new spoligotype, this outbreak adds up to previous reports suggesting that sheep tuberculosis could be huge reservoir of infection worldwide overlooked by skin test low sensitivity or simply lack of investigation. This makes it urgent to extend the use antibody tests to address the Trojan horse of hidden M. tuberculosis complex infections on bovine TB control programs.
Treatment of corn with lactic acid delayed in vitro ruminal degradation without compromising fermentation: a biological and morphological monitoring study
Authors: Tian K. E. , Luo Gan , Aldian Dicky , Yayota Masato
Published: January 2024

Grain processed by lactic acid (LA) is known to improve ruminant growth and health. However, the exact mechanism regarding rumen hydrolysis of LA-treated grain is still ambiguous. This experiment was designed to compare the effects of 5% LA treatment on the trophic and morphological variations in corn and to discover the alternations in ruminal hydrolysis between LA-treated and untreated corn macroscopically and microscopically using in vitro fermentation method. The results showed that, compared with untreated corn (CN), corn treated with 5% LA for 48 h (CNLA) experienced a decrease in the dry matter, albumin fraction, aNDFom, and water-soluble carbohydrate content but an increase in the resistant starch content. The in vitro fermentation showed that the pH of CNLA was higher, but dry matter disappearance was lower than that of CN. Most of the fermentation indices were unaffected, except for decreased iso-butyrate and iso-valerate. The abundances of total bacteria, Prevotella spp., Streptococcus bovis, and Selenomonas ruminantium were higher, but those of Ruminococcus flavefaciens and Ruminococcus albus were lower in CNLA than in CN. There were differences in the scanning electron micrographs between CNLA and CN after 3 h of fermentation. This study suggests that treating corn with LA for 48 h can induce changes in its nutrient composition and alter the bacterial flora during subsequent in vitro fermentation. These changes appeared to be crucial contributors to the beneficial effects observed in rumen fermentation.
The Advent of Semi-Elective Lung Transplantation—Prolonged Static Cold Storage at 10°C
Authors: Hoetzenecker K. , Benazzo A. , Schwarz S. , Keshavjee S. , Cypel M.
Published: January 2024

Since the early days of clinical lung transplantation the preservation of donor organs has become a fairly standardized procedure and most centers do follow similar processes. This includes the use of low-potassium high dextran flush solutions and static cold storage (SCS) in a cooler filled with ice. Depending on the length of SCS, organs usually arrive at the recipient hospital at a temperature of 0°C–4°C. The question of the optimal storage temperature for donor lung preservation has been revisited as data from large animal experiments demonstrated that organs stored at 10°C experience less mitochondrial damage. Thus, prolonged cold ischemic times can be better tolerated at 10°C—even in pre-damaged organs. The clinical applicability of these findings was demonstrated in an international multi-center observational study including three high-volume lung transplant centers. Total clinical preservation times of up to 24 hrs have been successfully achieved in organs stored at 10°C without hampering primary organ function and short-term outcomes. Currently, a randomized-controlled trial (RCT) is recruiting patients with the aim to compare standard SCS on ice with prolonged SCS protocol at 10°C. If, as anticipated, this RCT confirms data from previous studies, lung transplantation could indeed become a semi-elective procedure.
A Structural Design and Motion Characteristics Analysis of an Inchworm-Inspired Soft Robot Based on Shape Memory Alloy Actuation
Authors: Wei Qiong , Ke Ding , Sun Zihang , Wu Zilong , Zhou Yue , Zhang Daode
Published: January 2024

Inchworms are a widely adopted bio-inspired model for soft crawling robots. Taking advantage of the good controllability of Shape Memory Alloy (SMA), this paper designs and manufactures an inchworm-inspired soft robot driven by SMA. Firstly, in the structural design, the paper compares the heat dissipation performance and driving efficiency of SMA actuators with two assembly forms: embedded and external to the silicone body. The external structure assembly design with superior performance is chosen. Secondly, in the analysis of the motion characteristics of the soft robot, a kinematic model is developed. Addressing the issue of inaccurate representation in traditional constitutive models due to difficult-to-measure parameters, such as martensite volume fraction, this paper derives an exclusive new constitutive model starting from traditional models using methods like the Taylor series and thermodynamic laws. The kinematic model is simulated using the Simulink platform to obtain its open-loop step response and sinusoidal signal response. Finally, an experimental platform is set up to conduct crawling tests on the soft robot in different planes. The experimental results show that the inchworm-inspired soft robot can perform continuous crawling motion, with a crawling speed of 0.041 mm/s on sandpaper under a constant current of 4A.
Beneficial Soil Microbiomes and Their Potential Role in Plant Growth and Soil Fertility
Authors: Vincze Éva-Boglárka , Becze Annamária , Laslo Éva , Mara Gyöngyvér
Published: January 2024

The soil microbiome plays an important role in maintaining soil health, plant productivity, and soil ecosystem services. Current molecular-based studies have shed light on the fact that the soil microbiome has been quantitatively underestimated. In addition to metagenomic studies, metaproteomics and metatranscriptomic studies that target the functional part of the microbiome are becoming more common. These are important for a better understanding of the functional role of the microbiome and for deciphering plant-microbe interactions. Free-living beneficial bacteria that promote plant growth by colonizing plant roots are called plant growth-promoting rhizobacteria (PGPRs). They exert their beneficial effects in different ways, either by facilitating the uptake of nutrients and synthesizing particular compounds for plants or by preventing and protecting plants from diseases. A better understanding of plant-microbe interactions in both natural and agroecosystems will offer us a biotechnological tool for managing soil fertility and obtaining a high-yield food production system.
Effect of Pulsed Electric Field Treatment on Seed Germination and Seedling Growth of Scutellaria baicalensis
Authors: Song Yanbo , Zhao Weiyu , Su Zhenxian , Guo Shuhong , Du Yihan , Song Xinyue , Shi Xiaojing , Li Xiaofeng , Liu Yuli , Liu Zhenyu
Published: January 2024

To explore the effects of pulsed electric field treatment on the germination of Scutellaria baicalensis seeds and the growth of seedlings, this study used the response surface methodology to design the working parameters of the pulsed electric field and treated and cultured Scutellaria baicalensis seeds. The results showed that the pulsed electric field treatment was beneficial for the germination of Scutellaria baicalensis seeds, improving the metabolic activity and stress resistance of seedlings. When the pulsed electric field treatment’s parameters were 0.5 kV·cm−1, 120 μs, and 99 pulses, the germination potential of seeds was significantly increased by 29.25% and the germination index significantly increased by 20.65%, compared to the control. From 5th to 15th day, the activities of SOD, POD, and α-amylase in the seedlings, and the contents of Pro, soluble sugars, and soluble proteins were significantly increased, compared to the control. This study provides a theoretical basis for improving the germination and seedling growth of medicinal herbs such as Scutellaria baicalensis and their practical application in production.
Applying Remote Sensing, Sensors, and Computational Techniques to Sustainable Agriculture: From Grain Production to Post-Harvest
Authors: Rodrigues Dágila , Coradi Paulo , Timm Newiton , Fornari Michele , Grellmann Paulo , Amado Telmo , Teodoro Paulo , Teodoro Larissa , Baio Fábio , Chiomento José
Published: January 2024

In recent years, agricultural remote sensing technology has made great progress. The availability of sensors capable of detecting electromagnetic energy and/or heat emitted by targets improves the pre-harvest process and therefore becomes an indispensable tool in the post-harvest phase. Therefore, we outline how remote sensing tools can support a range of agricultural processes from field to storage through crop yield estimation, grain quality monitoring, storage unit identification and characterization, and production process planning. The use of sensors in the field and post-harvest processes allows for accurate real-time monitoring of operations and grain quality, enabling decision-making supported by computer tools such as the Internet of Things (IoT) and artificial intelligence algorithms. This way, grain producers can get ahead, track and reduce losses, and maintain grain quality from field to consumer.