bluuebunny/crossref_metadata_2025 huggingface dataset:
SIDGAN: Efficient Multi-Module Architecture for Single Image Defocus Deblurring
Authors: Ling Shenggui , Zhan Hongmin , Cao Lijia
Published: June 2024

In recent years, with the rapid developments in deep learning and graphics processing units, learning-based defocus deblurring has made favorable achievements. However, the current methods are not effective in processing blurred images with a large depth of field. The greater the depth of field, the blurrier the image, namely, the image contains large blurry regions and encounters severe blur. The fundamental reason for the unsatisfactory results is that it is difficult to extract effective features from the blurred images with large blurry regions. For this reason, a new FFEM (Fuzzy Feature Extraction Module) is proposed to enhance the encoder’s ability to extract features from images with large blurry regions. After using the FFEM during encoding, its PSNR (Peak Signal-to-Noise Ratio) is improved by 1.33% on the DPDD (Dual-Pixel Defocus Deblurring). Moreover, images with large blurry regions often cause the current algorithms to generate artifacts in their results. Therefore, a new module named ARM (Artifact Removal Module) is proposed in this work and employed during decoding. After utilizing the ARM during decoding, its PSNR is improved by 2.49% on the DPDD. After using the FFEM and the ARM simultaneously, compared to the latest algorithms, the PSNR of our method is improved by 3.29% on the DPDD. Following the previous research in this field, qualitative and quantitative experiments are conducted on the DPDD and the RealDOF (Real Depth of Field), and the experimental results indicate that our method surpasses the state-of-the-art algorithms in three objective metrics.
Energy Cost Optimization for Incorporating Energy Hubs into a Smart Microgrid with RESs, CHP, and EVs
Authors: Anastasiadis Anestis G. , Lekidis Alexios , Pierros Ioannis , Polyzakis Apostolos , Vokas Georgios A. , Papageorgiou Elpiniki I.
Published: June 2024

The energy carrier infrastructure, including both electricity and natural gas sources, has evolved and begun functioning independently over recent years. Nevertheless, recent studies are pivoting toward the exploration of a unified architecture for energy systems that combines Multiple-Energy Carriers into a single network, hence moving away from treating these carriers separately. As an outcome, a new methodology has emerged, integrating electrical, chemical, and heating carriers and centered around the concept of Energy Hubs (EHs). EHs are complex systems that handle the input and output of different energy types, including their conversion and storage. Furthermore, EHs include Combined Heat and Power (CHP) units, which offer greater efficiency and are more environmentally benign than traditional thermal units. Additionally, CHP units provide greater flexibility in the use of natural gas and electricity, thereby offering significant advantages over traditional methods of energy supply. This article introduces a new approach for exploring the steady-state model of EHs and addresses all related optimization issues. These issues encompass the optimal dispatch across multiple carriers, the optimal hub interconnection, and the ideal hub configuration within an energy system. Consequently, this article targets the reduction in the overall system energy costs, while maintaining compliance with all the necessary system constraints. The method is applied in an existing Smart Microgrid (SM) of a typical Greek 17-bus low-voltage distribution network into which EHs are introduced along with Renewable Energy Sources (RESs) and Electric Vehicles (EVs). The SM experiments focus on the optimization of the operational cost using different operational scenarios with distributed generation (DG) and CHP units as well as EVs. A sensitivity analysis is also performed under variations in electricity costs to identify the optimal scenario for handling increased demand.
Short-Term Load Forecasting of Electric Vehicle Charging Stations Accounting for Multifactor IDBO Hybrid Models
Authors: Tang Minan , Wang Changyou , Qiu Jiandong , Li Hanting , Guo Xi , Sheng Wenxin
Published: June 2024

The charging behavior of electric vehicle users is highly stochastic, which makes the short-term prediction of charging load at electric vehicle charging stations difficult. In this paper, a data-driven hybrid model optimized by the improved dung beetle optimization algorithm (IDBO) is proposed to address the problem of the low accuracy of short-term prediction. Firstly, the charging station data are preprocessed to obtain clear and organized load data, and the input feature matrix is constructed using factors such as temperature, date type, and holidays. Secondly, the optimal CNN-BiLSTM model is constructed using convolutional neural network (CNN) and Bi-directional Long Short-Term Memory (BiLSTM), which realizes the feature extraction of the input matrix and better captures the hidden patterns and regularities in it. Then, methods such as Bernoulli mapping are used to improve the DBO algorithm and its hyperparameters; for example, hidden neurons of the hybrid model are tuned to further improve the model prediction accuracy. Finally, a simulation experiment platform is established based on MATLAB R2023a to validate the example calculations on the historical data of EV charging stations in the public dataset of ANN-DATA, and comparative analyses are carried out. The results show that compared with the traditional models such as CNN, BiLSTM and PSO-CNN-BiLSTM, the coefficient of determination of the model exceeds 0.8921 and the root mean square error is maintained at about 4.413 on both the training and test sets, which proves its effectiveness and stability.
Study on Discharge Characteristic Performance of New Energy Electric Vehicle Batteries in Teaching Experiments of Safety Simulation under Different Operating Conditions
Authors: Gong Meilin , Chen Jiatao , Chen Jianming , Zhao Xiaohuan
Published: June 2024

High-voltage heat release from batteries can cause safety issues for electric vehicles. Relevant scientific research work is carried out in the laboratory. The battery safety of laboratory experiments should not be underestimated. In order to evaluate the safety performance of batteries in the laboratory testing of driving conditions of electric vehicles, this paper simulated and compared the discharge characteristics of two common batteries (lithium iron phosphate (LFP) battery and nickel–cobalt–manganese (NCM) ternary lithium battery) in three different operating conditions. The operating conditions are the NEDC (New European Driving Cycle), WLTP (World Light Vehicle Test Procedure) and CLTC-P (China light vehicle test cycle) for normal driving of electric vehicles. LFP batteries have a higher maximum voltage and lower minimum voltage under the same initial voltage conditions, with a maximum voltage difference variation of 11 V. The maximum current of WLTP is significantly higher than NEDC and CLTC-P operating conditions (>20 A). Low current discharge conditions should be emulated in teaching simulation and experiments for safety reasons. The simulation data showed that the LFP battery had good performance in maintaining the voltage plateau and discharge voltage stability, while the NCM battery had excellent energy density and long-term endurance.
Performance Analysis of Vermiculite–Potassium Carbonate Composite Materials for Efficient Thermochemical Energy Storage
Authors: Lin Jianquan , Zhao Qian , Huang Haotian
Published: June 2024

In this study, the preparation of the composite material consisting of expanded vermiculite (EV) and potassium carbonate (K2CO3) was conducted using a solution impregnation method. Sorption and desorption experiments were undertaken to investigate the dynamic and thermodynamic properties of the EV/K2CO3 composites with varying salt contents. The findings suggest that the EV/K2CO3 composites effectively address the issues of solution leakage resulting from the deliquescence and excessive hydration of pure K2CO3 salt, thereby substantially improving the water sorption capacity and overall stability of the composite materials. The salt content plays a vital role in the sorption and desorption processes of EV/K2CO3 composites. As the salt content rises, the resistance to sorption mass transfer increases, resulting in a decline in the average sorption rate. Concurrently, as the salt content increases, there is a corresponding increase in the average desorption rate, water uptake, and heat storage density. Specifically, at a temperature of 30 °C and a relative humidity of 60%, the EVPC40 composite with a salt content of 67.4% demonstrates water uptake, mass energy density, and volumetric energy density values of 0.68 g/g, 1633.6 kJ/kg, and 160 kWh/m3, respectively. In comparison to pure K2CO3 salt, the utilization of EV/K2CO3 composites under identical heat demand conditions results in a 57% reduction in the required reaction material. This study offers essential empirical evidence and theoretical backing for the utilization and development of EV/K2CO3 composites within thermochemical energy storage systems.
Multizone Modeling for Hybrid Thermal Energy Storage
Authors: Jäger Sarah , Pabst Valerie , Renze Peter
Published: June 2024

This study presents a one-dimensional mathematical model developed to simulate multi-zone thermal storage systems using phase change materials (PCMs). The model enables precise analysis of temperature distribution in the layered storage based on several PCM configurations and properties. It is distinguished by its adaptability to various tank geometries and the number of PCM capsules, enabling its application under diverse operating conditions. By simplifying the implementation of heat transfer processes that depend on the shape of the capsule and the thermal properties of the PCM, the computation time can be reduced to a level that makes simulations over longer periods feasible. Experimental validation confirmed the accuracy of the model, with deviations below 6%, underscoring its practical applicability. The study demonstrates that individual layering in the storage tank can be achieved by filling it with PCMs of different melting points without compromising the maximum storage capacity. It is shown that including a PCM layer can maintain the outlet temperature 20% longer while storing 14% more energy. The results point out the model’s potential to improve the performance of thermal storage systems through targeted PCM layer configurations. The model serves as the basis for the planning and optimization of these systems.
Evaluation of the Properties and Reaction-to-Fire Performance of Binderless Particleboards Made from Canary Island Palm Trunks
Authors: Ferrandez-Garcia Berta Elena , Garcia-Ortuño Teresa , Ferrandez-Villena Manuel , Ferrandez-Garcia Maria Teresa
Published: June 2024

Repurposing agricultural and forestry by-products not only is beneficial for the environment but also follows the principles of the circular economy. In southeastern Spain, the Canary Island palm tree (Phoenix canariensis W.) is widely used in urban landscapes. Plantations affected by the red weevil, a pest, generate an abundance of plant waste that must be crushed and transferred to authorized landfills. The aim of this study was to manufacture boards using particles from trunks of the Canary Island palm tree without adding any binders in order to obtain an ecological and fire-resistant product. In order to manufacture the boards, three particle sizes (<0.25, 0.25–1, and 1–2 mm), a temperature of 110 °C, a pressure of 2.6 MPa, and a pressing time of 7 min were used. The boards were pressed in a hot plate press for 7 min up to four times (7 min, 7 + 7 min, 7 + 7 + 7 min, and 7 + 7 + 7 + 7 min). The resulting boards showed good thermal performance, and the board´s reaction-to-fire performance was classified as Bd0 (an Fs value of 70.3 mm). This study also showed that boards with a particle size smaller than 0.25 mm that underwent four pressing cycles of 7 min each in the press can be categorized as grade P2 according to the European Standards (MOR of 20 N/mm2, MOE of 2589.8 N/mm2, and IB of 0.74 N/mm2). Therefore, these manufactured particleboards could be used as a flame-retardant material for the interior enclosures of buildings (vertical and horizontal) without the need for coatings.
Harnessing Hue: Advances and Applications of Fish Skin Pigmentation Genetics in Aquaculture
Authors: Liu Jialong , Yin Miaomiao , Ye Zhi , Hu Jingjie , Bao Zhenmin
Published: June 2024

Fish exhibit a broad spectrum of colors and patterns facilitated by specialized cells known as chromatophores. The vibrant coloration of fish, controlled by complex genetic and environmental interactions, serves critical roles in ecological functions such as mating, predation, and camouflage. This diversity not only makes fish an invaluable model for exploring the molecular mechanisms of pigmentation but also significantly impacts their economic value within the aquaculture industry, where color traits can drive marketability and breeding choices. This review delves into the sophisticated biological processes governing fish pigmentation and discusses their applications in enhancing aquaculture practices. By exploring the intersection of genetic regulation, environmental influences, and advanced breeding techniques, this review highlights both the scientific understanding and practical applications of fish coloration, providing a bridge between basic biological research and its application in commercial aquaculture.
Exploring Texture and Biomechanics of Food Oral Processing in Fork-Mashable Dishes for Patients with Mastication or Swallowing Impairments
Authors: Ismael-Mohammed Kovan , Bolívar-Prados Mireia , Laguna Laura , Nuñez Lara Adrian , Clavé Pere
Published: June 2024

Texture-modified diets (TMDs) are a primary compensatory treatment for hospitalized older patients with swallowing and mastication disorders. Nevertheless, the lack of a protocol for evaluating their objective textural properties hampers their industrialization and optimal patient care. Objectives: This study aimed (a) to evaluate the textural properties (maximum force, cohesiveness, and adhesiveness) and biomechanics of food oral processing (mastication cycles, time, and frequency) of ten fork-mashable dishes (Texture E BDA/IDDSI level 6), (b) to explore the impact of oral processing on texture, and (c) to measure the properties of the ready-to-swallow bolus (RSB) in healthy adults. Methods: The textural properties (maximum force, cohesiveness, and adhesiveness) of ten dishes were analyzed with a texture analyzer before and after oral processing (RSB) in five healthy adults (30 ± 3.9, 3 women). Surface electromyography was used to measure mastication cycles, time, and frequency. Results: The pre-mastication Texture Profile Analysis (TPA)-averaged values of maximum force ranged from 0.65 to 2.73 N, cohesiveness was 0.49–0.87, and adhesiveness was 0.01–0.95 N·s. Masticatory Cycles (46.87–19.13 MC) and time (36.73–15.80 S) from whole samples to RSB greatly and significantly differed among dishes, although frequency did not (1.68–1.11 MC/T). Post-mastication RSB TPA-averaged values of maximum force ranged from 0.70 to 2.24 N; cohesiveness, 0.49–0.73; and adhesiveness, 0.01–1.14 N·s. Conclusions: Despite all dishes being classified by the same qualitative descriptor (BDA level E/IDDSI level 6), there was a large and significant variation in their textural properties (maximum force, cohesiveness, and adhesiveness) when measured in SI units. In addition, in healthy adults, the masticatory cycles and time to achieve RSB greatly differed, whereas masticatory frequency remained quite constant.
Preparation and Characterization of Chitosan/Hydroxypropyl Methylcellulose Temperature-Sensitive Hydrogel Containing Inorganic Salts for Forest Fire Suppression
Authors: Gao Yanni , Zhao Yuzhou , Wang Ting
Published: June 2024

Effective forest fire suppression remains a critical challenge, necessitating innovative solutions. Temperature-sensitive hydrogels represent a promising avenue in this endeavor. Traditional firefighting methods often struggle to address forest fires efficiently while mitigating ecological harm and optimizing resource utilization. In this study, a novel intelligent temperature-sensitive hydrogel was prepared specially for forest fire extinguishment. Utilizing a one-pot synthesis approach, this material demonstrates exceptional fluidity at ambient temperatures, facilitating convenient application and transport. Upon exposure to elevated temperatures, it undergoes a phase transition to form a solid, barrier-like structure essential for containing forest fires. The incorporation of environmentally friendly phosphorus salts into the chitosan/hydroxypropyl methylcellulose gel system enhances the formation of temperature-sensitive hydrogels, thereby enhancing their structural integrity and firefighting efficacy. Morphological and thermal stability analyses elucidate the outstanding performance, with the hydrogel forming a dense carbonized layer that acts as a robust barrier against the spread of forest fires. Additionally, comprehensive evaluations employing rheological tests, cone calorimeter tests, a swelling test, and infrared thermography reveal the multifaceted roles of temperature-sensitive hydrogels in forest fire prevention and suppression strategies.
Higher Education Institutions as Strategic Centers for Promoting Social Innovation in Gerontology: Insights from the Senior Innovation Lab Training Initiative
Authors: Feijóo-Quintas Susana , Gerbaudo-González Noelia , Gandoy-Crego Manuel , Gutiérrez-Moar Mª del Carmen , Costa Elísio , Facal David
Published: June 2024

Background: Social innovation and gerontology develop a wide range of actions aimed at supporting and improving the needs of long-lived populations. Higher education institutions (HEIs) are drivers of change, and their potential to develop solutions through teaching students’ social innovation skills should be considered in the field of longevity. This article reports the results obtained by the Senior Innovation Lab (SIL) training initiative. Methods: Challenge-based learning, design thinking, and lean startup approaches were implemented in training 26 participants with the final aim of developing innovative solutions to previously identified long-lived population needs. Results: Final products were innovative ideas developed through collaboration between students, academic staff, and business employees, indicating the importance of adopting entrepreneurial approaches in academic teaching. The participants identified motivation and perseverance as the most relevant entrepreneurial skills and most of them also perceived that they possessed it. The participants also considered spotting opportunities (chance to add value) as the most desired skill throughout the whole experience. Conclusions: SIL’s assessment showed that social innovation methodologies contributed to the development of learning strategies, enabling potential solutions for the ageing challenges.
Particularities of Cataract Surgery in Elderly Patients: Corneal Structure and Endothelial Morphological Changes after Phacoemulsification
Authors: Ciorba Adela Laura , Teusdea Alin , Roiu George , Cavalu Daniela Simona
Published: June 2024

The aim of this study was to evaluate the influence of ultrasounds used in phacoemulsification during cataract surgery on the corneal structure and morphology in patients over 65 years. We compared the outcomes of phacoemulsification techniques in terms of corneal cell morphology in 77 patients over 65 years old and 43 patients under 65 years old. Corneal cell density, central corneal thickness and hexagonality were measured preoperatively and post-surgery (at 1 and 4 weeks) by specular microscopy. The effect of gender, axial length and anterior chamber depth on the parameters of corneal endothelium were evaluated. In both groups, a progressive decrease in endothelial cells was observed, starting from the first week post-surgery until the fourth postoperative week. The central corneal thickness increased in both groups with maximum values at the first week postoperatively, while their initial values were restored in the fourth week post-surgery, with no statistical difference between groups. Statistically significant differences were noticed in terms of cell hexagonality in the group over 65, showing smaller hexagonality at all preoperative and postoperative time points compared to group under 65. Our result highlights the importance of routine specular microscopy performed before surgery, regardless the age of the patients, with caution and careful attention to the phaco power intensity, ultrasound energy consumption and intraoperative manipulation of instruments, as well as proper use of viscoelastic substances to reduce corneal endothelium damage, especially in elderly patients.
A Comprehensive Examination of Age-Related Lower Limb Muscle Function Asymmetries across a Variety of Muscle Action Types
Authors: Heap-Eldridge Kylee L. , Thompson Brennan J. , Fisher Cody , Louder Talin J. , Carey Jon
Published: June 2024

Previous research has found that lower limb muscle asymmetries increase with age and are linked to fall and injury risks. However, past studies lack a wide variety of muscle function modes and measures as well as comparison to a comparable younger age group. The purpose of this study was to examine age-related lower limb muscle function asymmetries across a variety of muscle action types and velocities in young and old adults. Lower limb balance, strength, power, and velocity were evaluated with concentric, isometric, isotonic, and eccentric muscle actions during a single-leg stance test and on single- and multi-joint dynamometers in 29 young (age = 21.45 ± 3.02) and 23 old (age = 77.00 ± 4.60) recreationally active men and women. Most (15 of 17) variables showed no statistical (p > 0.05) or functional (10% threshold) limb asymmetry for either age group. There was a significant main effect (p = 0.046; collapsed across groups) found for asymmetry (dominant > non-dominant) for the isotonic peak velocity variable. There was a significant (p = 0.010) group × limb interaction for single-joint concentric peak power produced at a slow (60 deg/s) velocity due to the non-dominant limb of the young group being 12.2% greater than the dominant limb (p < 0.001), whereas the old group was not asymmetrical (p = 0.965). The findings of this investigation indicate there is largely no age-related asymmetry of the lower limbs across a range of muscle function-related variables and modes, with a couple of notable exceptions. Also, the significant asymmetries for the isotonic peak velocity variable perhaps show the sensitivity of this uncommonly used measure in detecting minimally present muscle function imbalances.
Do Atopic Dermatitis and Psoriasis Have an Impact on Cognitive Decline—Latest Research Review
Authors: Kuryłło Marcin , Mojs Ewa
Published: June 2024

Background: Atopic dermatitis and psoriasis are chronic skin diseases that affect the mental health of patients. The relationship between AD and psoriasis and cognitive processes in patients remains unclear. The aim of the review was to answer the question of whether AD and psoriasis have an impact on cognitive decline in patients. Method: A systematic literature search was conducted on PubMed and EBSCO to identify case–control, cross-sectional, or cohort studies that evaluated the association between atopic dermatitis and psoriasis and cognitive impairment. Results: Most of the studies included in the review confirmed cognitive decline in patients with atopic dermatitis and psoriasis. Conclusions: It seems that atopic dermatitis and psoriasis may negatively affect cognitive processes such as working memory, concentration, attention, and speed of motor reactions. Psychological interventions targeting distorted cognitive processing could improve the quality of life of patients with atopic dermatitis and psoriasis.
Contribution of EBSD for the Microstructural Study of Archaeological Iron Alloy Artefacts from the Archaeological Site of Loiola (Biscay, Northern Spain)
Authors: Rémazeilles Céline , Zuluaga Maria Cruz , Portillo-Blanco Haizea , Conforto Egle , Oudriss Abdelali , Ortega Luis Àngel , Alonso-Olazabal Ainhoa , Cepeda-Ocampo Juan José
Published: June 2024

Iron palaeometallurgy was carried out on three artefacts, classified as nails and excavated from the archaeological site of Loiola (La Arboleda, Biscay, northern Spain), to investigate Roman manufacturing techniques. Energy Dispersive Spectroscopy (EDS) coupled with Environmental Scanning Electron Microscopy (ESEM) and micro-Raman spectroscopy were used to obtain elemental composition and structural characterization of mineral phases. Metallurgical properties and crystallographic texture were studied by combining microscopic methods such as optical microscopy (OM), Electron Backscatter Diffraction realized in environmental mode (EBSD) and measurements of local Vickers microhardness. The three artefacts had different microstructures, distinguished by a large gradient of carbon content, although important segregations (inclusions) were observed in all of them. Two pearlite-rich artefacts showed a high density of structural defects (geometrically necessary dislocations and large crystallographic orientation gradients in pearlitic ferrite, curved pearlitic cementite) resulting from a high level of plastic deformation that occurred during the manufacturing process. The third artefact consisted of pure ferrite without structural defects. This one was clearly manufactured differently from the two others, so it probably had another functionality.
Integration of RNA-Seq and Metabolite Analysis Reveals the Key Floral Scent Biosynthetic Genes in Herbaceous Peony
Authors: Kimani Shadrack Kanyonji , Wang Shuxian , Xie Jinyi , Bao Tingting , Shan Xiaotong , Li Hongjie , Adnan  , Wang Li , Gao Xiang , Li Yueqing
Published: June 2024

Floral scent is an essential and genetically complex trait in herbaceous peonies (Paeonia lactiflora Pall.); however, specific genes related to metabolic and regulatory networks remain scantily studied. Our study integrated metabolite profiling and RNA-sequencing to screen floral scent biosynthetic genes. Hence, the major molecules identified by headspace collection combined with cultivar-specific GC-MS analysis were geraniol, β-caryophyllene, 2-phenylethanol (2-PE), citronellol, and 1,8-cineole. Genes related to terpenoids and 2-PE biosynthesis were identified after the assembly and annotation of the P. lactiflora transcriptomes. Eight angiosperm-specific terpene synthases (TPSs) from the TPS-a and TPS-b clades, as well as enzymes linked to 2-PE synthesis such as aromatic amino acid decarboxylase (AADC), phenylacetaldehyde reductase (PAR), and geranial reductase (GER) were identified. The biochemical analysis of the enzymes encoded by PlPAR1 and PlGER1 generated 2-PE from phenylacetaldehyde (PAld). The pairwise alignment of AADC1 reveals a splice variant lacking a 124 bp fragment, thus highlighting the possible role of alternative splicing in modulating floral scent composition. This study offers insights into the molecular-level biosynthesis of terpenoids and 2-PE in Peonia taxa, and provides the basis for the functional characterization, breeding, and bioengineering of prospective candidate genes for the production of floral volatiles in the Paeonia genus.
Adolescents’ Tsunami Exposure and Mental Health Consequences: Protective Role of Cultural Coping Strategies
Authors: Wickrama Thulitha , Merten Michael J. , Wickrama K. A. S. , Terrell Amanda
Published: June 2024

There is a knowledge gap regarding the link between disaster exposure and adolescent mental health problems in developing countries. This study examines the case of Sri Lanka to investigate (a) the immediate and long-term mental health impact of the 2004 tsunami disaster on adolescents and (b) the potential moderating effects of unique cultural and family practices that prevail in Sri Lanka. This study used a random sample of 160 adolescents (ages 12–19) and their mothers who were exposed to the tsunami disaster while living in a southern Sri Lankan village and provided prospective data immediately after the disaster (2005) and three years later (2008). A cross-culturally validated instrument assessed adolescent–mother dyads’ tsunami exposure, stressful loss, family cultural rituals and familism, and adolescent mental health. Structural equation modeling analysis showed that exposure and perceptions of tsunami-induced stressful experiences were associated with early and later mental health problems in adolescents. In addition, this study found that unique cultural practices and familism moderated the link between adolescent tsunami exposure, stressful experiences, and levels of PTSD and depressive symptoms. The findings of this study could be utilized to develop prevention and intervention programs that are contextually and culturally valid and empirically supported, which would be more effective for trauma-exposed adolescents in developing countries.
Arithmetic vs. Weighted Means in Fish Fillets Mercury Analyses
Authors: Heinonen-Tanski Helvi
Published: June 2024

Mercury (Hg) analyses in species of fish are performed for two reasons: (1) to safeguard human health; and (2) to assess environmental quality, since different environmental changes may increase the Hg concentrations in fish. These analyses are important since both natural and human activities can increase these Hg concentrations, which can vary extensively, depending on the species, age and catching location. Hg-contaminated fish or other marine foodstuffs can be only detected by chemical analysis. If the aim of Hg analysis is to protect the health of marine food consumers, researcher workers must consider the location where the fish were caught and interpret the results accordingly. Health and environmental officials must appreciate that in specific places, local people may have a daily diet consisting entirely of fish or other marine foods, and these individuals should not be exposed to high concentrations of Hg. Regional and national health and environmental officials should follow the recent guidance of international organizations when drawing their final conclusions about whether the products are safe or unsafe to eat. Correct statistical calculations are not always carried out; so, too high Hg amounts could be presented, and fish eaters could be protected. This work has been conducted to show the differences in Hg concentrations between weighted (weighted with fish weights) and arithmetic means. Thus, the mean that is only weighted also includes the Hg content in fishes; so, the exposure to Hg can be evaluated.
Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis
Authors: Gosetti di Sturmeck Tommaso , Malimpensa Leonardo , Ferrazzano Gina , Belvisi Daniele , Leodori Giorgio , Lembo Flaminia , Brandi Rossella , Pascale Esterina , Cattaneo Antonino , Salvetti Marco , Conte Antonella , D’Onofrio Mara , Arisi Ivan
Published: June 2024

The current hypothesis on the pathophysiology of multiple sclerosis (MS) suggests the involvement of both inflammatory and neurodegenerative mechanisms. Disease Modifying Therapies (DMTs) effectively decrease relapse rates, thus reducing relapse-associated disability in people with MS. In some patients, disability progression, however, is not solely linked to new lesions and clinical relapses but can manifest independently. Progression Independent of Relapse Activity (PIRA) significantly contributes to long-term disability, stressing the urge to unveil biomarkers to forecast disease progression. Twenty-five adult patients with relapsing–remitting multiple sclerosis (RRMS) were enrolled in a cohort study, according to the latest McDonald criteria, and tested before and after high-efficacy Disease Modifying Therapies (DMTs) (6–24 months). Through Agilent microarrays, we analyzed miRNA profiles from peripheral blood mononuclear cells. Multivariate logistic and linear models with interactions were generated. Robustness was assessed by randomization tests in R. A subset of miRNAs, correlated with PIRA, and the Expanded Disability Status Scale (EDSS), was selected. To refine the patient stratification connected to the disease trajectory, we computed a robust logistic classification model derived from baseline miRNA expression to predict PIRA status (AUC = 0.971). We built an optimal multilinear model by selecting four other miRNA predictors to describe EDSS changes compared to baseline. Multivariate modeling offers a promising avenue to uncover potential biomarkers essential for accurate prediction of disability progression in early MS stages. These models can provide valuable insights into developing personalized and effective treatment strategies.
Pathogenesis and Therapy of Oral Carcinogenesis
Authors: Tarle Marko , Lukšić Ivica
Published: June 2024

Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the head and neck with an extremely poor five-year survival rate of approximately 50 to 55%, despite significant advances in diagnostic and therapeutic procedures over the past three decades [...]
CD52/FLAG and CD52/HA Fusion Proteins as Novel Magnetic Cell Selection Markers
Authors: Kandarakov Oleg F. , Polyakova Natalia S. , Petrovskaya Alexandra V. , Bruter Alexandra V. , Belyavsky Alexander V.
Published: June 2024

At present, the magnetic selection of genetically modified cells is mainly performed with surface markers naturally expressed by cells such as CD4, LNGFR (low affinity nerve growth factor receptor), and MHC class I molecule H-2Kk. The disadvantage of such markers is the possibility of their undesired and poorly predictable expression by unmodified cells before or after cell manipulation, which makes it essential to develop new surface markers that would not have such a drawback. Earlier, modified CD52 surface protein variants with embedded HA and FLAG epitope tags (CD52/FLAG and CD52/HA) were developed by the group of Dr. Mazurov for the fluorescent cell sorting of CRISPR-modified cells. In the current study, we tested whether these markers can be used for the magnetic selection of transduced cells. For this purpose, appropriate constructs were created in MigR1-based bicistronic retroviral vectors containing EGFP and DsRedExpress2 as fluorescent reporters. Cytometric analysis of the transduced NIH 3T3 cell populations after magnetic selection evaluated the efficiency of isolation and purity of the obtained populations, as well as the change in the median fluorescence intensity (MFI). The results of this study demonstrate that the surface markers CD52/FLAG and CD52/HA can be effectively used for magnetic cell selection, and their efficiencies are comparable to that of the commonly used LNGFR marker. At the same time, the significant advantage of these markers is the absence of HA and FLAG epitope sequences in cellular proteins, which rules out the spurious co-isolation of negative cells.
The Key Role of Plant Hormone Signaling Transduction and Flavonoid Biosynthesis Pathways in the Response of Chinese Pine (Pinus tabuliformis) to Feeding Stimulation by Pine Caterpillar (Dendrolimus tabulaeformis)
Authors: Zhao Yanan , Sun Tianhua , Liu Jie , Zhang Ruibo , Yu Yongjie , Zhou Guona , Liu Junxia , Gao Baojia
Published: June 2024

In nature, plants have developed a series of resistance mechanisms to face various external stresses. As understanding of the molecular mechanisms underlying plant resistance continues to deepen, exploring endogenous resistance in plants has become a hot topic in this field. Despite the multitude of studies on plant-induced resistance, how plants respond to stress under natural conditions remains relatively unclear. To address this gap, we investigated Chinese pine (Pinus tabuliformis) using pine caterpillar (Dendrolimus tabulaeformis) under natural conditions. Healthy Chinese pine trees, approximately 10 years old, were selected for studying induced resistance in Huangtuliangzi Forestry, Pingquan City, Chengde City, Hebei Province, China. Pine needles were collected at 2 h and 8 h after feeding stimulation (FS) via 10 pine caterpillars and leaf clipping control (LCC), to simulate mechanical damage caused by insect chewing for the quantification of plant hormones and transcriptome and metabolome assays. The results show that the different modes of treatments significantly influence the contents of JA and SA in time following treatment. Three types of differentially accumulated metabolites (DAMs) were found to be involved in the initial response, namely phenolic acids, lipids, and flavonoids. Weighted gene co-expression network analysis indicated that 722 differentially expressed genes (DEGs) are positively related to feeding stimulation and the specific enriched pathways are plant hormone signal transduction and flavonoid biosynthesis, among others. Two TIFY transcription factors (PtTIFY54 and PtTIFY22) and a MYB transcription factor (PtMYB26) were found to be involved in the interaction between plant hormones, mainly in the context of JA signal transduction and flavonoid biosynthesis. The results of this study provide an insight into how JA activates, serving as a reference for understanding the molecular mechanisms of resistance formation in conifers responding to mandibulate insects.
Genome-Wide Identification of the TGA Gene Family and Expression Analysis under Drought Stress in Brassica napus L.
Authors: Duan Yi , Xu Zishu , Liu Hui , Wang Yanhui , Zou Xudong , Zhang Zhi , Xu Ling , Xu Mingchao
Published: June 2024

TGA transcription factors belong to Group D of the bZIP transcription factors family and play vital roles in the stress response of plants. Brassica napus is an oil crop with rich economic value. However, a systematic analysis of TGA gene family members in B. napus has not yet been reported. In this study, we identified 39 full-length TGA genes in B. napus, renamed TGA1~TGA39. Thirty-nine BnTGA genes were distributed on 18 chromosomes, mainly located in the nucleus, and differences were observed in their 3D structures. Phylogenetic analysis showed that 39 BnTGA genes could be divided into five groups. The BnTGA genes in the same group had similar structure and motif compositions, and all the BnTGA genes had the same conserved bZIP and DOG1 domains. Phylogenetic and synteny analysis showed that the BnTGA genes had a close genetic relationship with the TGA genes of the Brassica juncea, and BnTGA11 and BnTGA29 may play an important role in evolution. In addition, qRT-PCR revealed that three genes (BnTGA14/17/23) showed significant changes in eight experimental materials after drought treatment. Meanwhile, it can be inferred from the results of drought treatment on different varieties of rapeseed that the stress tolerance of parental rapeseed can be transmitted to the offspring through hybridization. In short, these findings have promoted the understanding of the B. napus TGA gene family and will contribute to future research aimed at B. napus resistant breeding.
Homozygosity for a Rare Plec Variant Suggests a Contributory Role in Congenital Insensitivity to Pain
Authors: Kantaputra Piranit , Daroontum Teerada , Kitiyamas Kantapong , Piyakhunakorn Panat , Kawasaki Katsushige , Sathienkijkanchai Achara , Wasant Pornswan , Vatanavicharn Nithiwat , Yasanga Thippawan , Kaewgahya Massupa , Tongsima Sissades , Cox Timothy C. , Arold Stefan T. , Ohazama Atsushi , Ngamphiw Chumpol
Published: June 2024

Congenital insensitivity to pain is a rare human condition in which affected individuals do not experience pain throughout their lives. This study aimed to identify the molecular etiology of congenital insensitivity to pain in two Thai patients. Clinical, radiographic, histopathologic, immunohistochemical, and molecular studies were performed. Patients were found to have congenital insensitivity to pain, self-mutilation, acro-osteolysis, cornea scars, reduced temperature sensation, tooth agenesis, root maldevelopment, and underdeveloped maxilla and mandible. The skin biopsies revealed fewer axons, decreased vimentin expression, and absent neurofilament expression, indicating lack of dermal nerves. Whole exome and Sanger sequencing identified a rare homozygous variant c.4039C>T; p.Arg1347Cys in the plakin domain of Plec, a cytolinker protein. This p.Arg1347Cys variant is in the spectrin repeat 9 region of the plakin domain, a region not previously found to harbor pathogenic missense variants in other plectinopathies. The substitution with a cysteine is expected to decrease the stability of the spectrin repeat 9 unit of the plakin domain. Whole mount in situ hybridization and an immunohistochemical study suggested that Plec is important for the development of maxilla and mandible, cornea, and distal phalanges. Additionally, the presence of dental anomalies in these patients further supports the potential involvement of Plec in tooth development. This is the first report showing the association between the Plec variant and congenital insensitivity to pain in humans.
The Therapeutic Effects of Blueberry-Treated Stem Cell-Derived Extracellular Vesicles in Ischemic Stroke
Authors: Jang Eunjae , Yu Hee , Kim Eungpil , Hwang Jinsu , Yoo Jin , Choi Jiyun , Jeong Han-Seong , Jang Sujeong
Published: June 2024

An ischemic stroke, one of the leading causes of morbidity and mortality, is caused by ischemia and hemorrhage resulting in impeded blood supply to the brain. According to many studies, blueberries have been shown to have a therapeutic effect in a variety of diseases. Therefore, in this study, we investigated whether blueberry-treated mesenchymal stem cell (MSC)-derived extracellular vesicles (B-EVs) have therapeutic effects in in vitro and in vivo stroke models. We isolated the extracellular vesicles using cryo-TEM and characterized the particles and concentrations using NTA. MSC-derived extracellular vesicles (A-EVs) and B-EVs were round with a lipid bilayer structure and a diameter of ~150 nm. In addition, A-EVs and B-EVs were shown to affect angiogenesis, cell cycle, differentiation, DNA repair, inflammation, and neurogenesis following KEGG pathway and GO analyses. We investigated the protective effects of A-EVs and B-EVs against neuronal cell death in oxygen–glucose deprivation (OGD) cells and a middle cerebral artery occlusion (MCAo) animal model. The results showed that the cell viability was increased with EV treatment in HT22 cells. In the animal, the size of the cerebral infarction was decreased, and the behavioral assessment was improved with EV injections. The levels of NeuN and neurofilament heavy chain (NFH)-positive cells were also increased with EV treatment yet decreased in the MCAo group. In addition, the number of apoptotic cells was decreased with EV treatment compared with ischemic animals following TUNEL and Bax/Bcl-2 staining. These data suggested that EVs, especially B-EVs, had a therapeutic effect and could reduce apoptotic cell death after ischemic injury.
Hypertriglyceridemia: Molecular and Genetic Landscapes
Authors: Scicchitano Pietro , Amati Francesca , Ciccone Marco Matteo , D’Ascenzi Flavio , Imbalzano Egidio , Liga Riccardo , Paolillo Stefania , Pastore Maria Concetta , Rinaldi Andrea , Mattioli Anna Vittoria , Cameli Matteo
Published: June 2024

Lipid disorders represent one of the most worrisome cardiovascular risk factors. The focus on the impact of lipids on cardiac and vascular health usually concerns low-density lipoprotein cholesterol, while the role of triglycerides (TGs) is given poor attention. The literature provides data on the impact of higher plasma concentrations in TGs on the cardiovascular system and, therefore, on the outcomes and comorbidities of patients. The risk for coronary heart diseases varies from 57 to 76% in patients with hypertriglyceridemia. Specifically, the higher the plasma concentrations in TGs, the higher the incidence and prevalence of death, myocardial infarction, and stroke. Nevertheless, the metabolism of TGs and the exact physiopathologic mechanisms which try to explain the relationship between TGs and cardiovascular outcomes are not completely understood. The aims of this narrative review were as follows: to provide a comprehensive evaluation of the metabolism of triglycerides and a possible suggestion for understanding the targets for counteracting hypertriglyceridemia; to describe the inner physiopathological background for the relationship between vascular and cardiac damages derived from higher plasma concentrations in TGs; and to outline the need for promoting further insights in therapies for reducing TGs plasma levels.
Being Stung Once or Twice by Bees (Apis mellifera L.) Slightly Disturbed the Serum Metabolome of SD Rats to a Similar Extent
Authors: Wang Xinyu , Zheng Xing , Wang Xue , Ji Quanzhi , Peng Wenjun , Liu Zhenxing , Zhao Yazhou
Published: June 2024

In most cases, the number of honeybee stings received by the body is generally small, but honeybee stings can still cause serious allergic reactions. This study fully simulated bee stings under natural conditions and used 1H Nuclear Magnetic Resonance (1H NMR) to analyze the changes in the serum metabolome of Sprague–Dawley (SD) rats stung once or twice by honeybees to verify the impact of this mild sting on the body and its underlying mechanism. The differentially abundant metabolites between the blank control rats and the rats stung by honeybees included four amino acids (aspartate, glutamate, glutamine, and valine) and four organic acids (ascorbic acid, lactate, malate, and pyruvate). There was no separation between the sting groups, indicating that the impact of stinging once or twice on the serum metabolome was similar. Using the Principal Component Discriminant Analysis ( PCA-DA) and Variable Importance in Projection (VIP) methods, glucose, lactate, and pyruvate were identified to help distinguish between sting groups and non-sting groups. Metabolic pathway analysis revealed that four metabolic pathways, namely, the tricarboxylic acid cycle, pyruvate metabolism, glutamate metabolism, and alanine, aspartate, and glutamate metabolism, were significantly affected by bee stings. The above results can provide a theoretical basis for future epidemiological studies of bee stings and medical treatment of patients stung by honeybees.
Molecular Study of Sudden Cardiac Death
Authors: Hostiuc Sorin
Published: June 2024

The aim of the Special Issue “Molecular study of sudden cardiac death” was to gather new studies on the molecular biology of cardiac death, from both a fundamental and clinical perspective [...]
The Role of Z Chromosome Localization Gene psmd9 in Spermatogenesis of Cynoglossus semilaevis
Authors: Zhang Yuman , Wang Yue , Liu Qian , Wang Hongyan , Wang Qian , Shao Changwei
Published: June 2024

Proteasome 26S Subunit, Non-ATPase 9 (psmd9) plays an important role in the balance of protamine and the stability of the nucleolar structure during spermatogenesis. In this study, we cloned the psmd9 of Cynoglossus semilaevis and analyzed its expression pattern. psmd9 was identified on the Z chromosome of C. semilaevis, which is considered an interesting candidate gene for spermatogenesis. qRT-PCR and FISH experiments showed that the psmd9 gene was significantly highly expressed in the testes. It is worth noting that the expression level of psmd9 in male fish testes is significantly higher than that in pseudomales. In order to further explore the role of psmd9 in spermatogenesis, a male testicular cell line was used as the experimental material. The results of the psmd9-RNAi and overexpression experiments showed that psmd9 had a synergistic effect with spermatogenesis-related genes dnd1, cfap69, dnah3 and dnajb13, but had an antagonistic effect with ccne2. Our findings offer a scientific foundation for comprehending the role of psmd9 in the spermatogenesis regulatory network of C. semilaevis.
The Intriguing World of Vascular Remodeling, Angiogenesis, and Arteriogenesis
Authors: Quax Paul H. A. , Deindl Elisabeth
Published: June 2024

Vascular remodeling is a very general feature related to angiogenesis and arteriogenesis, which are involved in neovascularization processes [...]