bluuebunny/crossref_metadata_2025 huggingface dataset:
Oral Rehabilitation of Patients Sustaining Orofacial Injuries: The UPenn Initiative
Authors: Zhang Q.Z. , Chen C. , Chang M.B. , Shanti R.M. , Cannady S.B. , O’Malley B.W. , Shi S. , Le A.D.
Published: October 2019

Tissue injuries in the oral and maxillofacial structures secondary to trauma, warfare, ablative cancer, and benign tumor surgery result in significant losses of speech, masticatory and swallowing functions, aesthetic deformities, and overall psychological stressors and compromise. Optimal oral rehabilitation remains a formidable challenge and an unmet clinical need due to the influence of multiple factors related to the physiologic limitations of tissue repair, the lack of site and function-specific donor tissues and constructs, and an integrated team of multidisciplinary professionals. The advancements in stem cell biology, biomaterial science, and tissue engineering technologies, particularly the 3-dimensional bioprinting technology, together with digital imaging and computer-aided design and manufacturing technologies, have paved the path for personalized/precision regenerative medicine. At the University of Pennsylvania, we have launched the initiative to integrate multidisciplinary health professionals and translational/clinical scientists in medicine, dentistry, stem cell biology, tissue engineering, and regenerative medicine to develop a comprehensive, patient-centered approach for precision and personalized reconstruction, as well as oral rehabilitation of patients sustaining orofacial tissue injuries and defects, especially oral cancer patients.
Dynamic characterisation of pressure transducers using shock tube methods
Authors: Knott Andy J , Robinson Ian A
Published: October 2019

This paper describes the characterisation of the dynamic response of a range of pressure transducer systems. The transducers were subjected to virtually instantaneous pressure step inputs in the National Physical Laboratory’s shock tube facilities. The magnitudes of these pressure steps were derived from ideal gas theory, with prior commissioning tests having been performed to demonstrate the theory’s validity in this application. The results demonstrate a significant variation in response obtained from various combinations of transducer, instrumentation settings, and mounting arrangement.
Population Diversity and Ancestral Diversity As Distinct Contributors to Outgroup Prejudice
Authors: Shrira Ilan
Published: October 2019

Previous research has shown conflicting findings on how population diversity influences outgroup prejudice. In some cases, prejudice is greater when minority groups make up a larger portion of the population, whereas in other cases, prejudice is lower as diversity increases. This article examined how the diversity of a culture’s ancestry—or its historical heterogeneity—would be related to outgroup attitudes. Historically heterogeneous populations descend from ancestors who have migrated from many parts of the world over the past 500 years and, as a result, have a longer legacy of contact with diverse groups of people. The results of two cross-cultural studies found that greater heterogeneity predicted lower levels of outgroup prejudice, and some evidence that diversity in the current population was related to increased prejudice. The findings suggest that intergroup attitudes have deeply entrenched roots that cannot be fully understood by looking at current indicators.
Data Performativity and Health: The Politics of Health Data Practices in Europe
Authors: Blouin Gabriel G.
Published: October 2019

The European Commission produces the European Core Health Indicators (ECHI), a database containing different tools used to compare European Union (EU) countries and recommend policy changes. The ECHI feeds multiple reports and documents and finds its way into health policies. From this arises the main research question addressed in this paper: How is health in Europe influenced by ECHI data practices? Specifically, we look at how some health issues or populations are prioritized or dismissed, which ultimately shapes the meaning of and knowledge about health in Europe. To do so, we first develop the conceptual framework of “data performativity,” underlining how data practices shape their object/subject. We then explore the politics of evidence behind the ECHI health data that materialize into (1) the absence of some health issues and populations and (2) the hypervisibility of neoliberal health. In the end, we argue, the ECHI serves as a site of individual, collective, and political identity enunciation.
Anticipating a Second Wave of Standards-Based Grading Implementation and Understanding the Potential Barriers: Perceptions of High School Principals
Authors: Townsley Matt , Buckmiller Tom , Cooper Robyn
Published: October 2019

As secondary school leaders consider a shift toward standards-based grading (SBG) practices, they are no doubt weighing the odds of a successful implementation process. This research followed up on a study from 2014 to identify the challenges secondary school leaders experience when changing the currency of the classroom from points to learning. The results indicated that the game is changing and a new wave of SBG implementation is on the horizon.
Development and Validation of a Short Form of the Child–Adolescent Perfectionism Scale
Authors: Bento Carmen , Pereira Ana Telma , Azevedo Julieta , Saraiva Jorge , Flett Gordon L. , Hewitt Paul L. , Macedo Antonio
Published: October 2019

The objective of the present study was to develop and assess the validity of a short form of the Child–Adolescent Perfectionism Scale (CAPS). Two Portuguese samples composed of 756 adolescents were used to cross-validate the factorial structure of a nine-item Child–Adolescent Perfectionism Scale—Short Form (CAPS–SF). The CAPS–SF consists of a four-item self-oriented perfectionism subscale and a five-item socially prescribed perfectionism subscale. Both subscales demonstrated adequate internal consistency. Confirmatory factor analysis (CFA) of the CAPS–SF supported the same two-factor structure and represented a very good fit to the data for both groups. Other analyses found that the brief measure of socially prescribed perfectionism was associated with measures of distress and forms of self-criticism. The CAPS–SF appears to represent a reliable and valid alternative to the original CAPS. Overall, the CAPS-SF is considerably briefer than the original CAPS and it offers an economical and valid alternative when measuring perfectionism in children and adolescents.
How to Create Objects With Your Mind: From Object-Based Attention to Attention-Based Objects
Authors: Ongchoco Joan Danielle K. , Scholl Brian J.
Published: October 2019

When staring at a blank grid, one can readily “see” simple shapes—a peculiar experience that does not occur when viewing an empty background. But just what does this “seeing” entail? Previous work has explored many cues to object-based attention (e.g., involving continuity and closure), but here we asked whether attention can be object based even when there are no cues to objecthood. Observers viewed simple grids and attended to particular squares until they could effectively “see” shapes such as a capital H or I. During this scaffolded attention, two probes appeared, and observers reported whether they were the same or different. Remarkably, this produced a traditional same-object advantage: In several experiments (including high-powered direct replications), performance was enhanced for probes presented on the same (purely imagined) object, compared with equidistant probes presented on different objects. We conclude that attention not only operates over objects but also can effectively create object representations.
Synergistic application of operational modal analysis and ambient noise deconvolution interferometry for structural and damage identification in historic masonry structures: three case studies of Italian architectural heritage
Authors: García-Macías Enrique , Kita Alban , Ubertini Filippo
Published: October 2019

Conservation techniques within the framework of structural health monitoring, particularly through dynamic measurements and operational modal analysis, are becoming popular for condition-based maintenance and decision-making in historic structures. Nonetheless, while effective for giving insight into the overall behaviour of structures, these techniques may fail at detecting local damages with limited effects on the modal features of the system. In this regard, the analysis of propagating waves throughout the structure poses an attractive alternative for data-driven damage identification. Specifically, some encouraging results have been reported on the application of seismic interferometry to reinforced concrete structures, albeit the number of works concerning ambient vibrations is far scarce, and practically nonexistent in the realm of historic structures. In this light, this article explores the synergistic application of operational modal analysis and ambient noise deconvolution interferometry for the structural identification of historic structures through three different case studies, namely the Sciri Tower in Perugia, the Consoli Palace in Gubbio and the bell-tower of the Basilica of San Pietro in Perugia. The first case study represents a typical example of a masonry tower inserted into a building aggregate, while the second one constitutes a particular case of a monumental masonry palace. The presented results and discussion cover diverse aspects of the identification of wave velocities, signal processing strategies, effects of dispersion and robustness of the identification. Finally, the case study of the bell-tower of the Basilica of San Pietro illustrates the application of operational modal analysis and deconvolution interferometry for damage identification. To do so, two different ambient vibration tests conducted before and after the 2016 Central Italy seismic sequence are studied. The results show concentrated reductions in the wave velocities in the area of the belfry, which demonstrates that deconvolution interferometry constitutes a complementary technique to operational modal analysis for damage localization and, to some extent, damage quantification.
Beyond Maternal Mortality: A Systematic Review of Evidences on Mistreatment and Disrespect During Childbirth in Health Facilities in India
Authors: Jungari Suresh , Sharma Baby , Wagh Dhananjay
Published: October 2019

The aim of this systematic review is to examine current evidence on the nature and extent of disrespect and abuse (D&A), mistreatment and practices of respectful maternity care of women during childbirth in India. Electronic databases were searched for published studies relevant to the topic. The search was conducted from May to September 2018. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to conduct the review. A results synthesis was done using the Bowser and Hill landscape analytical framework for D&A of women during childbirth. Eleven studies are included in this review of which six were cross-sectional, four were qualitative, and one used a mixed-method approach. The type of abuse most frequently reported was the lack of respect and dignity (nondignified care) experienced by the women, usually in the form of negative and unfriendly attitudes of the providers. The least frequent form of mistreatment was physical abuse and detention in the facilities. The frequency of reported D&A was high, ranging from 10% to 77.3%. These behaviors were influenced by lack of education and empowerment of the women, their low socioeconomic status, poor training of providers and supervision, and a lack of accountability. Overall, disrespectful and abusive behavior had adverse impact on the utilization of health facilities for childbirth. It created a psychological distance between women and health providers. To our knowledge, this is the first systematic literature review to be conducted on respectful maternity care in India.
Silencing PSME3 induces colorectal cancer radiosensitivity by downregulating the expression of cyclin B1 and CKD1
Authors: Song Wen , Guo Cuiping , Chen Jianxiong , Duan Shiyu , Hu Yukun , Zou Ying , Chi Honggang , Geng Jian , Zhou Jun
Published: October 2019

Resistance to radiotherapy remains a severe obstacle in the treatment of high-risk colorectal cancer patients. Recent studies have indicated that proteasome activator complex subunit 3 (PSME3) participates in the development and progression of various human malignancies and is proposed to play a role in tumor radioresistance. However, the impact of PSME3 on radioresistance of colorectal cancer has been largely unknown. In the present study, the enhanced expression of PSME3 was observed in colorectal cancer cells and tissue. Upregulation of PSME3 was significantly implicated in lymph node state, lymphovascular invasion, and Dukes' stage. Furthermore, high PSME3 expression was closely linked to poorer overall and progression-free survival in patients with colorectal cancer. The study further demonstrated that the proliferative, invasive and migratory potential of colorectal cancer cells was effectively inhibited in vitro after silencing PSME3. Our results verified that knockdown of PSME3 probably triggered cell cycle arrest at the G2/M phase by downregulation of cyclinB1 and CDK1, thereby enhancing the radiosensitivity of colorectal cancer cells. These data illustrated that PSME3 is a promising biomarker predictive of colorectal cancer prognosis and silencing of PSME3 may provide with a new approach for sensitizing the radiotherapy in colorectal cancer.

Impact statement

It is reported that colorectal cancer (CRC) is the third most common cancer worldwide and the fourth leading cause of cancer-related death. At present, the main treatment method of colorectal cancer is surgery, supplemented by radiotherapy and chemotherapy. Among them, radiotherapy plays an important role in the treatment of locally advanced colorectal cancer, surgery, and chemotherapy. Our study found that down-regulation of PSME3 may enhance the radiosensitivity of CRC cells by triggering cell cycle arrest, which suggests that silence PSME3 may provide a new method for improving the radiosensitivity of CRC. What’more, our research also demonstrated that PSME3 may promote proliferation, invasive and migratory potential of CRC cells, which implies that PSME3 might be a biomarker of CRC for early diagnosis and treatment.
An Internet of Things infrastructure for gait characterization in assisted living environments and its application in the discovery of associations between frailty and cognition
Authors: González Iván , Navarro Fco Javier , Fontecha Jesús , Cabañero-Gómez Luis , Hervás Ramón
Published: October 2019

This article presents an easy-to-deploy and low-cost Internet of Things infrastructure for gait characterization based on a set of wireless inertial sensors, called nodes, connected to the same local area network. These nodes allow acquiring inertial raw data from the trunk of each frail elder involved in explicit gait trials carried out directly in the elderly care homes. The Internet of Things infrastructure has been validated for Quantitative Gait Analysis showing an adequate accuracy in the demarcation of relevant gait events and in the estimation of stride interval variability. The latter, in combination with other characteristics that are commonly used to assess the state of frail elders and which come from anthropometric, biological, nutritional, functional, and mobility domains, allows us to perform a cross-sectional cohort study and a subsequent multiple logistic regression to evaluate their impact on cognitive functioning. The cohort study and the multivariate regression are performed using a sample of 81 frail elders from two nursing homes in Spain. The results obtained indicate that frail elders aged 90 years or older, with moderate dependence in daily functioning and with a stride interval gait variability greater than 6%, were most likely to suffer cognitive impairment, representing what is called cognitive frail.
School Nurses on the Front Lines of Medicine: When You Don’t Need to “STOP AT AN ER STAT!”—The Role of Urgent Care Centers in the Management of Students With Nonemergent Chief Complaints
Authors: Rothstein Rachel , Zhen Kevin , Olympia Robert P.
Published: October 2019

In the midst of our nation’s growing primary care provider shortage and subsequent overutilization of the emergency room (ER), urgent care centers represent an accessible, convenient, and affordable alternative for the management of “urgencies” or nonemergent illnesses and injuries. Despite the presence of competent healthcare providers and a wide array of services at urgent care centers, patients continue to pursue care in the ER for urgencies. School nurses may utilize urgent care centers for the evaluation and management of students with nonemergent medical or traumatic chief complaints. This article discusses the field of urgent care medicine, describes the pediatric services provided at urgent care centers, and summarizes the capabilities of centers to deal with potential urgencies versus emergencies in children and adolescents based on their chief complaint.
The efficacy of herbal medicines on anxiety and depression in peri- and postmenopausal women: A systematic review and meta-analysis
Authors: Shahmohammadi Afshar , Ramezanpour Negar , Mahdavi Siuki Mahsa , Dizavandi Fathemeh , Ghazanfarpour Masumeh , Rahmani Yousef , Tahajjodi Rezvan , Babakhanian Masoudeh
Published: October 2019

Objective

The main objective in this systematic review was to analyse herbal medicine interventions for anxiety and depression to detect possible benefits of herbal medicines in peri- and postmenopausal women.

Material and method

The trials published since inception to August 2017 were searched on the databases of MEDLINE, ISI Web of Science, Scopus and Cochran central register of controlled trials

Results

Twenty-one trials were included into systematic review. The anxiety score (standardized mean difference = −1.19; p = 0.04; six trials) and depression (standardized mean difference = −0.952; p = 0.023; five trials) was lower in the phytoestrogen group compared to the placebo. However, the heterogeneity was notably high among trials that assessed anxiety outcome (p < 0.0001; I2 = 97.12%) and depression (p < 0.0001; I2 = 94.51%), and we were unable to reduce the heterogeneity to below 90% after one with one exclusion of the studies; 1000 mg of fenugreek extract on depression and anxiety, 600 mg of Trigonella foenum‐graecum de‐husked seed extract on psychosocial symptoms, 0.4 mg/ml Maca extract on anxiety, 500 mg of Hop plant on depression and anxiety, 80 mg or more than 80 mg of red clover-derived isoflavones on psychological symptoms had significant effect. Non-significant effect was observed in black cohosh on anxiety. However, significant effect of black cohosh was seen on psychological symptoms. Fennel had a positive effect in both patients with anxiety or depression and healthy women. Flaxseed group showed a non-significant effect on psychological symptoms.

Conclusion

The present systematic review found that some of the herbal medicines may have good effect in the relief of the anxiety and the depression.
The Weaklaw Vent, SE Scotland: Metasomatism of eruptive products by carbo-hydro-fluids of probable mantle origin
Authors: Upton Brian G. J. , Odling Nic , Kirstein Linda A. , Underhill John R. , Puziewicz Jacek , Ntaflos Theodore , Bagiński Bogusław , Hillier Steve , Andersen Jens C. , Rollinson Gavin K. , Perrillat Jean-Philippe
Published: October 2019

The Weaklaw vent in SE Scotland (East Lothian coast), inferred to be Namurian, produced lava spatter and volcanic bombs. The latter commonly contained ultramafic xenoliths. All were metasomatised by carbonic fluids rich in incompatible elements. The lavas and xenoliths are inferred to have been basanites and lherzolites prior to metasomatism. The abundance and size of (carbonated) peridotite xenoliths at Weaklaw denotes unusual rapidity of magma ascent and high-energy eruption making Weaklaw exceptional in the British Isles. The lavas and xenoliths were altered subsequently by low-temperature (<200°C) carbo-hydrous fluids to carbonate, clay and quartz assemblages. A small irregular tuffisite ‘dyke’ that transects the ejecta is also composed dominantly of carbonates and clays. The peridotitic xenoliths are typically foliated, interpreted as originating as pre-entrainment mantle shear-planes.

Analyses of the relic spinels shows them to be compositionally similar to spinels in local unaltered lherzolites from near-by basanitic occurrences. Chromium showed neither significant loss nor gain but was concentrated in a di-octahedral smectite allied to volkonskoite. It is in the complex association of smectite with other clays, chlorite and possibly fuchsite that the diverse incompatible elements are concentrated.

We conclude that late Palaeozoic trans-tensional fault movement caused mantle shearing. Rapid ascent of basanite magma entrained large quantities of sheared lithospheric mantle. This was followed by ascent of an aggressive carbonate-/ hydroxyl-rich fluid causing pervasive metasomatism. The vent is unique in several ways: in its remarkable clay mineralogy and in displaying such high Cr-clays in a continental intra-plate setting; in being more productive in terms of its ‘cargo’ of peridotite xenoliths; in presenting an essentially un-eroded sequence of Namurian extrusives; and, not least, for giving evidence for post-eruptive, surface release of small-melt, deep-source fluids.
Using imputation-based whole-genome sequencing data to improve the accuracy of genomic prediction for combined populations in pigs
Authors: Song Hailiang , Ye Shaopan , Jiang Yifan , Zhang Zhe , Zhang Qin , Ding Xiangdong
Published: October 2019

Background

For genomic selection in populations with a small reference population, combining populations of the same breed or populations of related breeds is an effective way to increase the size of the reference population. However, genomic predictions based on single nucleotide polymorphism (SNP)-chip genotype data using combined populations with different genetic backgrounds or from different breeds have not shown a clear advantage over using within-population or within-breed predictions. The increasing availability of whole-genome sequencing (WGS) data provides new opportunities for combined population genomic prediction. Our objective was to investigate the accuracy of genomic prediction using imputation-based WGS data from combined populations in pigs. Using 80K SNP panel genotypes, WGS genotypes, or genotypes on WGS variants that were pruned based on linkage disequilibrium (LD), three methods [genomic best linear unbiased prediction (GBLUP), single-step (ss)GBLUP, and genomic feature (GF)BLUP] were implemented with different prior information to identify the best method to improve the accuracy of genomic prediction for combined populations in pigs.

Results

In total, 2089 and 2043 individuals with production and reproduction phenotypes, respectively, from three Yorkshire populations with different genetic backgrounds were genotyped with the PorcineSNP80 panel. Imputation accuracy from 80K to WGS variants reached 92%. The results showed that use of the WGS data compared to the 80K SNP panel did not increase the accuracy of genomic prediction in a single population, but using WGS data with LD pruning and GFBLUP with prior information did yield higher accuracy than the 80K SNP panel. For the 80K SNP panel genotypes, using the combined population resulted in a slight improvement, no change, or even a slight decrease in accuracy in comparison with the single population for GBLUP and ssGBLUP, while accuracy increased by 1 to 2.4% when using WGS data. Notably, the GFBLUP method did not perform well for both the combined population and the single populations.

Conclusions

The use of WGS data was beneficial for combined population genomic prediction. Simply increasing the number of SNPs to the WGS level did not increase accuracy for a single population, while using pruned WGS data based on LD and GFBLUP with prior information could yield higher accuracy than the 80K SNP panel.
Asian elephants exhibit post-reproductive lifespans
Authors: Chapman Simon N. , Jackson John , Htut Win , Lummaa Virpi , Lahdenperä Mirkka
Published: October 2019

Background

The existence of extended post-reproductive lifespan is an evolutionary puzzle, and its taxonomic prevalence is debated. One way of measuring post-reproductive life is with post-reproductive representation, the proportion of adult years lived by females after cessation of reproduction. Analyses of post-reproductive representation in mammals have claimed that only humans and some toothed whale species exhibit extended post-reproductive life, but there are suggestions of a post-reproductive stage for false killer whales and Asian elephants. Here, we investigate the presence of post-reproductive lifespan in Asian elephants using an extended demographic dataset collected from semi-captive timber elephants in Myanmar. Furthermore, we investigate the sensitivity of post-reproductive representation values to availability of long-term data over 50 years.

Results

We find support for the presence of an extended post-reproductive stage in Asian elephants, and that post-reproductive representation and its underlying demographic rates depend on the length of study period in a long-lived animal.

Conclusions

The extended post-reproductive lifespan is unlikely due to physiological reproductive cessation, and may instead be driven by mating preferences or condition-dependent fertility. Our results also show that it is crucial to revisit such population measures in long-lived species as more data is collected, and if the typical lifespan of the species exceeds the initial study period.
Exceptionally high rates of positive selection on the rbcL gene in the genus Ilex (Aquifoliaceae)
Authors: Yao Xin , Tan Yun-hong , Yang Jun-bo , Wang Yan , Corlett Richard T. , Manen Jean-François
Published: October 2019

Background

The genus Ilex (Aquifoliaceae) has a near-cosmopolitan distribution in mesic habitats from tropical to temperate lowlands and in alpine forests. It has a high rate of hybridization and plastid capture, and comprises four geographically structured plastid groups. A previous study showed that the plastid rbcL gene, coding for the large subunit of Rubisco, has a particularly high rate of non-synonymous substitutions in Ilex, when compared with other plant lineages. This suggests a strong positive selection on rbcL, involved in yet unknown adaptations. We therefore investigated positive selection on rbcL in 240 Ilex sequences from across the global range.

Results

The rbcL gene shows a much higher rate of positive selection in Ilex than in any other plant lineage studied so far (> 3000 species) by tests in both PAML and SLR. Most positively selected residues are on the surface of the folded large subunit, suggesting interaction with other subunits and associated chaperones, and coevolution between positively selected residues is prevalent, indicating compensatory mutations to recover molecular stability. Coevolution between positively selected sites to restore global stability is common.

Conclusions

This study has confirmed the predicted high incidence of positively selected residues in rbcL in Ilex, and shown that this is higher than in any other plant lineage studied so far. The causes and consequences of this high incidence are unclear, but it is probably associated with the similarly high incidence of hybridization and introgression in Ilex, even between distantly related lineages, resulting in large cytonuclear discordance in the phylogenies.
Modeling honey yield, defensive and swarming behaviors of Italian honey bees (Apis mellifera ligustica) using linear-threshold approaches
Authors: Andonov Sreten , Costa Cecilia , Uzunov Aleksandar , Bergomi Patrizia , Lourenco Daniela , Misztal Ignacy
Published: October 2019

Background

Genetic improvement of honey bees is more difficult compared to other livestock, due to the very different reproductive behavior. Estimation of breeding values requires specific adjustment and the use of sires in the pedigree is only possible when mating of queens and drones is strictly controlled. In the breeding program of the National Registry for Italian Queen Breeders and Bee Producers the paternal contribution is mostly unknown. As stronger modeling may compensate for the lack of pedigree information, we tested two models that differed in the way the direct and maternal effects were considered. The two models were tested using 4003 records for honey yield, defensive and swarming behaviors of Italian honey bee queens produced between 2002 and 2014. The first model accounted for the direct genetic effect of worker bees and the genetic maternal effect of the queen, whereas model 2 considered the direct genetic effect of the queen without maternal effect. The analyses were performed by linear (honey production) and threshold (defensive and swarming behavior) single-trait models; estimated genetic correlations among traits were obtained by a three-trait linear-threshold model.

Results

For all traits, the highest predictability (correlation between breeding values estimated with and without performance records) was obtained with model 2, where direct genetic effect of queens was considered. With this model, heritability estimates were 0.26 for honey yield, 0.36 for defensive behavior, and 0.34 for swarming behavior. Multi-trait estimation resulted in similar or higher heritability estimates for all traits. A low, positive genetic correlation (0.19) was found between honey yield and defensive behavior, whereas the genetic correlation between honey yield and swarming behavior was moderate (0.41). A strong, positive genetic correlation was found between defensive and swarming behaviors (0.62). Predictability for multi-trait evaluations was higher for honey yield (0.46) and defensive behavior (0.30) but almost identical for swarming behavior (0.45) compared to corresponding single-trait predictability.

Conclusions

Multi-trait evaluation using a model that accounts for the direct genetic effect of queen was the best approach for breeding value estimation of Italian honey bees. The results suggest a new direction for selection of linear and categorical traits in breeding programs where drone origin is unknown.
A high-resolution genomic composition-based method with the ability to distinguish similar bacterial organisms
Authors: Zhou Yizhuang , Zhang Wenting , Wu Huixian , Huang Kai , Jin Junfei
Published: October 2019

Background

Genomic composition has been found to be species specific and is used to differentiate bacterial species. To date, almost no published composition-based approaches are able to distinguish between most closely related organisms, including intra-genus species and intra-species strains. Thus, it is necessary to develop a novel approach to address this problem.

Results

Here, we initially determine that the “tetranucleotide-derived z-value Pearson correlation coefficient” (TETRA) approach is representative of other published statistical methods. Then, we devise a novel method called “Tetranucleotide-derivedZ-valueManhattanDistance” (TZMD) and compare it with the TETRA approach. Our results show that TZMD reflects the maximal genome difference, while TETRA does not in most conditions, demonstrating in theory that TZMD provides improved resolution. Additionally, our analysis of real data shows that TZMD improves species differentiation and clearly differentiates similar organisms, including similar species belonging to the same genospecies, subspecies and intraspecific strains, most of which cannot be distinguished by TETRA. Furthermore, TZMD is able to determine clonal strains with the TZMD = 0 criterion, which intrinsically encompasses identical composition, high average nucleotide identity and high percentage of shared genomes.

Conclusions

Our extensive assessment demonstrates that TZMD has high resolution. This study is the first to propose a composition-based method for differentiating bacteria at the strain level and to demonstrate that composition is also strain specific. TZMD is a powerful tool and the first easy-to-use approach for differentiating clonal and non-clonal strains. Therefore, as the first composition-based algorithm for strain typing, TZMD will facilitate bacterial studies in the future.
The interplay between miR156/SPL13 and DFR/WD40–1 regulate drought tolerance in alfalfa
Authors: Feyissa Biruk A. , Arshad Muhammad , Gruber Margaret Y. , Kohalmi Susanne E. , Hannoufa Abdelali
Published: October 2019

Background

DevelopingMedicago sativaL. (alfalfa) cultivars tolerant to drought is critical for the crop’s sustainable production. miR156 regulates various plant biological functions by silencing SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors.

Results

To understand the mechanism of miR156-modulated drought stress tolerance in alfalfa we used genotypes with altered expression levels of miR156, miR156-regulatedSPL13, andDIHYDROFLAVONOL-4-REDUCTASE(DFR) regulatingWD40–1. Previously we reported the involvement of miR156 in drought tolerance, but the mechanism and downstream genes involved in this process were not fully studied. Here we illustrate the interplay between miR156/SPL13 and WD40–1/DFR to regulate drought stress by coordinating gene expression with metabolite and physiological strategies. Low to moderate levels of miR156 overexpression suppressedSPL13and increasedWD40–1to fine-tuneDFRexpression for enhanced anthocyanin biosynthesis. This, in combination with other accumulated stress mitigating metabolites and physiological responses, improved drought tolerance. We also demonstrated that SPL13 binds in vivo to theDFRpromoter to regulate its expression.

Conclusions

Taken together, our results reveal that moderate relative miR156 transcript levels are sufficient to enhance drought resilience in alfalfa by silencingSPL13and increasingWD40–1expression, whereas higher miR156 overexpression results in drought susceptibility.
Epidemiology and risk factors of patients with types of acute coronary syndrome presenting to a tertiary care hospital in Sri Lanka
Authors: Ralapanawa Udaya , Kumarasiri Pallegoda Vithanage Ranjith , Jayawickreme Kushalee Poornima , Kumarihamy Prabashini , Wijeratne Yapa , Ekanayake Madhushanka , Dissanayake Chandira
Published: October 2019

Background

Acute Coronary Syndrome (ACS) is one of the leading causes of death worldwide and studies have shown higher mortality rates and premature death in South Asian countries. The occurrence and effect of risk factors differ by type ofACS.Epidemiological studies in the Sri Lankan population are limited.

Methods

This is a cross sectional descriptive study conducted at the Teaching Hospital Peradeniya, Sri Lanka among patients presenting with ACS. Data was collected by an interviewer administered structured questionnaire and epidemiological patterns and risk factors were analyzed.

Results

The sample of 300 patients had a mean age of 61.3+/− 12.6 and male sex showed higher association with all three type of ACS compared to female with aPvalue of 0.001. This study showed higher mean age of 62.2 ± 11.4 years amongst unstable angina (UA) patients and 61.9 ± 14.5 years amongst non ST elevation myocardial infarction (NSTEMI) patients compared to 59.2 ± 11.2 years for ST elevation myocardial infarction (STEMI) patients with no significant statistical difference (P = 0.246). Approximately 55.8% STEMI patients, 39.8% UA and 35.5% NSTEMI patients were smokers indicating a significant association between smoking and STEMI (P = 0.017). Nearly 54.5% STEMI, 35.4% UA and 32.7% NSTEMI patients consumed alcohol and there was a very strong association between alcohol consumption and STEMI (P = 0.006). Almost 51.8% NSTEMI patients, 47.8% UA patients and 29.9% STEMI patients had hypertension(HT) (P = 0.008) indicating significant association of HT with UA and NSTEMI. About 33.6% UA patients and 30.0% NSTEMI patients had DM whilst only 22.1% of STEMI patients had DM of no significance (p = 0.225). Around 15.0% patients with UA, 25.5% with NSTEMI and 11.7% with STEMI had dyslipidemia (P = 0.032). There was a very strong association between a past history of ACS or stable angina with NSTEMI and UA (P = 0.001).

Conclusion

Smoking and alcohol abuse are significantly associated with STEMI.Patients with NSTEMI or Unstable Angina had higher rates of hypertension and were more likely to have a history of ACS or stable angina than STEMI patients. Patients with NSTEMI were more likely than patients with STEMI or UA to have dyslipidemia.
Polypharmacy in older patients: identifying the need for support by a community pharmacist
Authors: Beuscart Jean-Baptiste , Petit Ségolène , Gautier Sophie , Wierre Patrick , Balcaen Thibaut , Lefebvre Jean-Marc , Kambia Nicolas , Bertoux Elisabeth , Mascaut Daniel , Barthélémy Christine , Cuny Damien , Puisieux François , Décaudin Bertrand
Published: October 2019

Background

The community pharmacist is a key player in medication reviews of older outpatients. However, it is not always clear which individuals require a medication review. The objective of the present study was to identify high-priority older patients for intervention by a community pharmacist.

Methods

As part of their final-year placement in a community pharmacy, pharmacy students conducted 10 interviews each with older adults (aged 65 or over) taking at least five medications daily. The student interviewer also offered to examine the patient’s home medicine cabinet. An interview guide was developed by an expert group to assess the difficulties in managing and taking medications encountered by older patients.

Results

The 141 students interviewed a total of 1370 patients (mean age: 81.5; mean number of medications taken daily: 9.3). Of the 1370 interviews, 743 (54.2%) were performed in the patient’s home, and thus also included an examination of the home medicine cabinet. Adverse events were reported by 566 (42.0%) patients. A total of 378 patients (27.6%) reported difficulties in preparing, administering and/or swallowing medications. The inspections of medicine cabinets identified a variety of shortcomings: poorly located cabinets (in 15.0% of inspections), medication storage problems (21.7%), expired medications (40.7%), potentially inappropriate medications (15.0%), several different generic versions of the same drug (19.9%), and redundant medications (20.4%).

Conclusions

In a community pharmacy setting, high-priority older patients for intervention by a community pharmacist can be identified by asking simple questions about difficulties in managing, administering, taking or storing medications.
Anticholinergic and benzodiazepine medication use and risk of incident dementia: a UK cohort study
Authors: Grossi Carlota M. , Richardson Kathryn , Fox Chris , Maidment Ian , Steel Nicholas , Loke Yoon K. , Arthur Antony , Myint Phyo Kyaw , Campbell Noll , Boustani Malaz , Robinson Louise , Brayne Carol , Matthews Fiona E. , Savva George M.
Published: October 2019

Background

Studies suggest that anticholinergic medication or benzodiazepine use could increase dementia risk. We tested this hypothesis using data from a UK cohort study.

Methods

We used data from the baseline (Y0), 2-year (Y2) and 10-year (Y10) waves of the Medical Research Council Cognitive Function and Ageing Study. Participants without dementia at Y2 were included (n = 8216). Use of benzodiazepines (including nonbenzodiazepine Z-drugs), anticholinergics with score 3 (ACB3) and anticholinergics with score 1 or 2 (ACB12) according to the Anticholinergic Cognitive Burden scale were coded as ever use (use at Y0 or Y2), recurrent use (Y0 and Y2), new use (Y2, but not Y0) or discontinued use (Y0, but not Y2). The outcome was incident dementia by Y10. Incidence rate ratios (IRR) were estimated using Poisson regression adjusted for potential confounders. Pre-planned subgroup analyses were conducted by age, sex and Y2 Mini-Mental State Examination (MMSE) score.

Results

Dementia incidence was 9.3% (N = 220 cases) between Y2 and Y10. The adjusted IRRs (95%CI) of developing dementia were 1.06 (0.72, 1.60), 1.28 (0.82, 2.00) and 0.89 (0.68, 1.17) for benzodiazepines, ACB3 and ACB12 ever-users compared with non-users. For recurrent users the respective IRRs were 1.30 (0.79, 2.14), 1.68 (1.00, 2.82) and 0.95 (0.71, 1.28). ACB3 ever-use was associated with dementia among those with Y2 MMSE> 25 (IRR = 2.28 [1.32–3.92]), but not if Y2 MMSE≤25 (IRR = 0.94 [0.51–1.73]).

Conclusions

Neither benzodiazepines nor ACB12 medications were associated with dementia. Recurrent use of ACB3 anticholinergics was associated with dementia, particularly in those with good baseline cognitive function. The long-term prescribing of anticholinergics should be avoided in older people.
Bridging the gap between research-based knowledge and clinical practice: a qualitative examination of patients and physiotherapists’ views on the Otago exercise Programme
Authors: Worum Hilde , Lillekroken Daniela , Ahlsen Birgitte , Roaldsen Kirsti Skavberg , Bergland Astrid
Published: October 2019

Background

Falls and fall-related injuries exacerbate the health problems of older adults, and they are a public health concern. Despite an abundance of research, the implementation of evidence-based fall prevention programs has been slow and limited, additionally and these programs have not reduced the incidence of falling. Therefore, the primary objective of the present study was to examine patients and physiotherapists’ views on the factors that influence the implementation of the community- and evidence-based Otago Exercise Programme for fall prevention.

Methods

We conducted eight in-depth interviews with physiotherapists and patients, and a focus group interview with 12 physiotherapists and authority figures who represented local hospitals and municipalities. The resultant qualitative data were subjected to thematic analysis.

Results

The analysis yielded two main themes:the researcher’s role and position in the implementation processandthe tension between research-based knowledge and clinical practice. The participants believed that research-based knowledge can address the challenges of clinical practice. Further, the patients reported that the fall prevention program made them feel safe and enhanced their ability to cope with daily life. The physiotherapists also observed that research findings do not readily translate into clinical practice. Further, they contended that research-based knowledge is not universal and that it cannot be generalized across different contexts; instead, it must be adapted and translated into a user-friendly language. The findings suggest that the application of research-based knowledge does equate tofilling up empty jarsand thatresearch-based knowledge does not flow from the expert to the non-expert as water through a tube.Indeed, physiotherapists and patients are nottabula rasa.Additionally, the participants believed that researchers and stakeholders must think critically about who has the power and voice to createa common understanding.

Conclusions

Our findings delineate the means by which the gap between research and practice regarding the Otago fall prevention program can bridged. The program can guide clinical work and provide important information that can be used to improve the quality of other fall prevention programs. However, the research-based knowledge that it confers must be adapted for use in clinical contexts.
A case report of disseminated nocardiosis with ocular involvement in a myasthenia gravis patient and literature review
Authors: Wang Shuhui , Jiang Bin , Li Yao , Shang Yanchang , Liu Zhengshan , Zhang Yongbo
Published: October 2019

Background

Nocardiosis is a rare and life-threatening opportunistic infection in immunocompromised patients. Myasthenia gravis (MG) patients are potentially at risk of nocardia infection because of the use of immunosuppressive agents. To date, only 7 patients with MG have been reported to have nocardiosis. Disseminated nocardiosis with ocular involvement has not been reported in MG patients.

Case presentation

A 66-year-old man with MG who was receiving treatment with methylprednisolone and azathioprine was found to have a respiratory infection. He also had heterogeneous symptoms with skin, brain and ocular manifestations. Nocardia bacteria verified by the culture of puncture fluid, and a diagnosis of disseminated nocardiosis was made. Except for left eye blindness, the patient completely recovered from the disease with combination antibiotic therapy. To further understand nocardiosis in patients with MG, we reviewed the previous relevant literature. According to the literature, this is the first report of disseminated nocardiosis with ocular involvement in an MG patient.

Conclusions

MG patients with immunosuppressant treatments are potentially at risk of a rare nocardia infection, and a favourable prognosis can be achieved through early diagnosis and appropriate antibiotic therapy.
Maternal health service utilisation of adolescent women in sub-Saharan Africa: a systematic scoping review
Authors: Mekonnen Tensae , Dune Tinashe , Perz Janette
Published: October 2019

Introduction

Sub-Saharan Africa has the highest rate of adolescent pregnancy in the world. While pregnancy during adolescence poses higher risks for the mother and the baby, the utilisation of maternity care to mitigate the effects is low. This review aimed to synthesise evidence on adolescent mothers’ utilisation of maternity care in Sub-Saharan Africa and identify the key determinant factors that influence adolescent mothers’ engagement with maternity care.

Method

A systematic review of scholarly literature involving seven databases: ProQuest, PubMed, EMBASE/Elsevier, SCOPUS, PsycINFO, CINAHL and Infomit was conducted. Studies published in English between 1990 and 2017 that examined Sub-Saharan adolescent mothers’ experiences of utilising biomedical maternity care during pregnancy, delivery and the post-partum period were included.

Results

From 296 relevant articles 27 were identified that represent the experience of adolescent mothers’ maternal health service utilisation in Sub-Saharan Africa. The review indicates that maternal health service utilisation in the majority of Sub-Saharan African countries is still low. There is also a wide discrepancy in the use of maternity care services by adolescent mothers across countries in Sub-Saharan Africa.

Conclusions

The review reveals that a significant number of adolescents in Sub-Saharan Africa do not access and use maternity services during pregnancy. Several factors from individual to systemic levels contributed to low access and utilisation. This implies that interventions targeting the women, their partners, healthcare professionals, communities and the organisations (local to national) are necessary to improve adolescent mother’s engagement with maternity care in Sub-Saharan Africa.