bluuebunny/crossref_metadata_2025 huggingface dataset:
Large Fragment InDels Reshape Genome Structure of Porcine Alveolar Macrophage 3D4/21 Cells
Authors: Li Xiaolong , Zhang Xiaoqian , Luo Yandong , Liu Ru , Sun Yan , Zhao Shuhong , Yu Mei , Cao Jianhua
Published: August 2022

The porcine monomyeloid cell line, or 3D4/21 cells, is an effective tool to study the immune characteristics and virus infection mechanism of pigs. Due to the introduction of the neomycin resistance gene and the SV40 large T antigen gene, its genome has undergone essential changes, which are still unknown. Studying the variation in genome structure, especially the large fragments of insertions and deletions (InDels), is one of the proper ways to reveal these issues. In this study, an All-seq method was established by combining Mate-pair and Shotgun sequencing methods, and the detection and verification of large fragments of InDels were performed on 3D4/21 cells. The results showed that there were 844 InDels with a length of more than 1 kb, of which 12 regions were deletions of more than 100 kb in the 3D4/21 cell genome. In addition, compared with porcine primary alveolar macrophages, 82 genes including the CD163 had lost transcription in 3D4/21 cells, and 72 genes gained transcription as well. Further referring to the Hi-C structure, it was found that the fusion of the topologically associated domains (TADs) caused by the deletion may lead to abnormal gene function. The results of this study provide a basis for elaborating the genome structure and functional variation in 3D4/21 cells, provide a method for rapid and convenient detection of large-scale InDels, and provide useful clues for the study of the porcine immune function genome and the molecular mechanism of virus infection.
Mechanism of SCD Participation in Lipid Droplet-Mediated Steroidogenesis in Goose Granulosa Cells
Authors: Yuan Xin , Abdul-Rahman Ibn Iddriss , Hu Shenqiang , Li Liang , He Hua , Xia Lu , Hu Jiwei , Ran Mingxia , Liu Yali , Abdulai Mariama , Wang Jiwen
Published: August 2022

Stearoyl-CoA desaturase (SCD) is a key enzyme catalyzing the rate-limiting step in monounsaturated fatty acids (MUFAs) production. There may be a mechanism by which SCD is involved in lipid metabolism, which is assumed to be essential for goose follicular development. For this reason, a cellular model of SCD function in goose granulosa cells (GCs) via SCD overexpression and knockdown was used to determine the role of SCD in GC proliferation using flow cytometry. We found that SCD overexpression induced and SCD knockdown inhibited GCs proliferation. Furthermore, ELISA analysis showed that SCD overexpression increased the total cholesterol (TC), progesterone, and estrogen levels in GCs, while SCD knockdown decreased TC, progesterone, and estrogen levels (p < 0.05). Combining these results with those of related multi-omics reports, we proposed a mechanism of SCD regulating the key lipids and differentially expressed gene (DEGs) in glycerophospholipid and glycerolipid metabolism, which participate in steroidogenesis mediated by the lipid droplet deposition in goose GCs. These results add further insights into understanding the lipid metabolism mechanism of goose GCs.
Comprehensive Analyses of Simple Sequence Repeat (SSR) in Bamboo Genomes and Development of SSR Markers with Peroxidase Genes
Authors: Liu Yan , Xiao Xiaoyan , Li Guangzhu , Zhu Chenglei , Yang Kebin , Feng Xiaohu , Lou Yongfeng , Gao Zhimin
Published: August 2022

Simple sequence repeats (SSRs) are one of the most important molecular markers, which are widespread in plants. Bamboos are important forest resources worldwide. Here, the comprehensive identification and comparative analysis of SSRs were performed in three woody and two herbaceous bamboo species. Altogether 567,175 perfect SSRs and 71,141 compound SSRs were identified from 5737.8 Mb genome sequences of five bamboo species. Di-nucleotide SSRs were the most predominant type, with an average of ~50,152.2 per species. Most SSRs were located in intergenic regions, while those located in genic regions were relatively less. Moreover, the results of annotation distribution indicated that terms with P450, peroxidase and ATP-binding cassette transporter related to lignin biosynthesis might play important roles in woody and herbaceous bamboos under the mediation of SSRs. Furthermore, the peroxidase gene family consisted of a large number of genes containing SSRs was selected for the evolutionary relationship analysis and SSR markers development. Fifteen SSR markers derived from peroxidase family genes of Phyllostachys edulis were identified as polymorphic in 34 accessions belonging to seven genera in Bambusoideae. These results provided a comprehensive insight of SSR markers into bamboo genomes, which would facilitate bamboo research related to comparative genomics, evolution and marker-assisted selection.
Complete Chloroplast Genome Features of Dendrocalamusfarinosus and Its Comparison and Evolutionary Analysis with Other Bambusoideae Species
Authors: Pei Jialong , Wang Yong , Zhuo Juan , Gao Huibin , Vasupalli Naresh , Hou Dan , Lin Xinchun
Published: August 2022

Dendrocalamus farinosus is one of the essential bamboo species mainly used for food and timber in the southwestern region of China. In this study, the complete chloroplast (cp) genome of D. farinosus is sequenced, assembled, and the phylogenetic relationship analyzed. The cp genome has a circular and quadripartite structure, has a total length of 139,499 bp and contains 132 genes: 89 protein-coding genes, eight rRNAs and 35 tRNAs. The repeat analyses showed that three types of repeats (palindromic, forward and reverse) are present in the genome. A total of 51 simple sequence repeats are identified in the cp genome. The comparative analysis between different species belonging to Dendrocalamus revealed that although the cp genomes are conserved, many differences exist between the genomes. The analysis shows that the non-coding regions were more divergent than the coding regions, and the inverted repeat regions are more conserved than the single-copy regions. Moreover, these results also indicate that rpoC2 may be used to distinguish between different bamboo species. Phylogenetic analysis results supported that D. farinosus was closely related to D. latiflorus. Furthermore, these bamboo species’ geographical distribution and rhizome types indicate two evolutionary pathways: one is from the tropics to the alpine zone, and the other is from the tropics to the warm temperate zone. Our study will be helpful in the determination of the cp genome sequences of D. farinosus, and provides new molecular data to understand the Bambusoideae evolution.
Mining High-Level Imaging Genetic Associations via Clustering AD Candidate Variants with Similar Brain Association Patterns
Authors: Wu Ruiming , Bao Jingxuan , Kim Mansu , Saykin Andrew J. , Moore Jason H. , Shen Li ,   on behalf of ADNI
Published: August 2022

Brain imaging genetics examines associations between imaging quantitative traits (QTs) and genetic factors such as single nucleotide polymorphisms (SNPs) to provide important insights into the pathogenesis of Alzheimer’s disease (AD). The individual level SNP-QT signals are high dimensional and typically have small effect sizes, making them hard to be detected and replicated. To overcome this limitation, this work proposes a new approach that identifies high-level imaging genetic associations through applying multigraph clustering to the SNP-QT association maps. Given an SNP set and a brain QT set, the association between each SNP and each QT is evaluated using a linear regression model. Based on the resulting SNP-QT association map, five SNP–SNP similarity networks (or graphs) are created using five different scoring functions, respectively. Multigraph clustering is applied to these networks to identify SNP clusters with similar association patterns with all the brain QTs. After that, functional annotation is performed for each identified SNP cluster and its corresponding brain association pattern. We applied this pipeline to an AD imaging genetic study, which yielded promising results. For example, in an association study between 54 AD SNPs and 116 amyloid QTs, we identified two SNP clusters with one responsible for amyloid beta clearances and the other regulating amyloid beta formation. These high-level findings have the potential to provide valuable insights into relevant genetic pathways and brain circuits, which can help form new hypotheses for more detailed imaging and genetics studies in independent cohorts.
Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers
Authors: Yacouba Zare , Isidore Houaga , Michel Kere , Isidore Gnanda B. , Boureima Traore , Vinsoun Millogo , Maurice Konkobo , Ousseni Bandaogo , Moussa Zongo , Valerie Bougouma-Yameogo M. C. , Romdhane Rekaya , Joseph Nianogo A.
Published: August 2022

The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogenetic relationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (FIS) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (FST) and among-individual differentiation (FIT), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso.
Occupational Stress and Personality in Medical Doctors from Romania
Authors: Muntean Lorena Mihaela , Nireștean Aurel , Mărușteri Marius , Sima-Comaniciu Andreea , Lukacs Emese
Published: August 2022

Occupational stress amongst doctors has been intensively studied as doctors are exposed to several stress factors daily. The purpose of this study was to investigate if there are associations between personality dimensions and the factors that generate stress at work. We conducted a cross-sectional study of 280 medical doctors from Romania between February 2021 and September 2021 who were evaluated using the DECAS and ASSET Scales. Our results showed that the agreeableness and emotional stability dimensions of personality, according to the Big Five model, were statistically associated with work relationships (A p < 0.0001; ES p = 0.0005), work-life balance (A p = 0.008; ES p = 0.01), overload (A p = 0.01; ES p = 0.001), job security (A p < 0.0001; ES p = 0.002), job control (A p = 0.001; ES p = 0.009), resources and communication (A p = 0.0002; ES p < 0.0001), and job conditions (A p = 0.005; ES p = 0.03). The conscientiousness dimension was statistically associated with job control (p = 0.02). Doctors from different specialties experienced stress differently, with psychiatrists and doctors from preclinical specialties reporting the lowest levels of stress. Internists and surgeons reported higher levels of stress. This study showed that the dimensions of agreeableness and emotional stability were both associated with variables indicative of the level of stress felt at work.
Effects of Home-Based Nine-Square Step Exercises for Fall Prevention in Thai Community-Dwelling Older Adults during a COVID-19 Lockdown: A Pilot Randomized Controlled Study
Authors: Dejvajara Disatorn , Aungkasuraphan Ranlaphat , Palee Piyathida , Piankusol Chanodom , Sirikul Wachiranun , Siviroj Penprapa
Published: August 2022

The deterioration of muscle strength in aging has been associated with fall risks. During the COVID-19 pandemic, older adults were restricted from doing outdoor activities. This study aimed to investigate the effect of Nine-Square Step Exercises (NSSE) on improving physical performance and balance in older adults at risk of falling. We conducted an open-labelled, assessor-blinded, randomized controlled trial in 46 (aged 65–84 years) community-dwelling older adults. They were randomly assigned to an NSSE group (n = 24) instructed to perform the program for at least 45 days over 8 weeks or a control group (n = 22). The outcomes were measured by the Timed Up and Go Test (TUG), the Berg Balance Scale (BBS), the Five-Times-Sit-to-Stand test (FTSS), and hand grip strength during the baseline, 4th and 8th weeks in both groups. A mixed-effect linear regression model analysis was performed to estimate the independent effect of NSSE by the intention-to-treat over the 8-week period. The NSSE group showed significant weekly changes in BBS (β 0.57, 95% CI: 0.30, 0.84), TUG (β −0.44, 95% CI: −0.74, −0.14), and FTSS (β −0.52, 95% CI: −0.78, −0.25), demonstrating beneficial improvements in lower extremity and balance, whereas the control group did not demonstrate significant changes over time in any parameter.
Evaluation of the Influence of Biological Factors during the Course of Treatment in Patients with Ovarian Cancer
Authors: Schneider-Matyka Daria , Skwirczyńska Edyta , Gaur Maria , Hukowska-Szematowicz Beata , Kwiatkowski Sebastian , Mikla Marzena , Grochans Elżbieta , Cymbaluk-Płoska Aneta
Published: August 2022

The aim of this study was to evaluate the influence of β-endorphins and serotonin on the course of treatment, disease-free time, and overall survival of patients with ovarian cancer. This study may contribute to the identification of modifiable factors that may influence the treatment of ovarian cancer. The research was carried out in a group of 162 patients of which 139 respondents were included in the research; ovarian cancer was diagnosed in 78 of these patients. The study consisted of three stages. In the first stage of diagnostics, a survey among the patients was carried out. In the second stage—5 mL of blood was collected from each patient (n = 139) in the preoperative period to determine the concentration of β-endorphin and serotonin. In the third stage—blood samples were collected from those patients who had completed chemotherapy treatment or had surgery. Concentrations of β-endorphin and serotonin were measured by the Luminex method, using the commercial Luminex Human Discovery Assay kit. The average age of the patients was 62.99 years. The level of β-endorphin significantly differs among patients diagnosed with ovarian cancer and among patients in the control group (202.86; SD—15.78 vs. 302.00; SD—24.49). A lower level of β-endorphins was found in the patients with a recurrence of the neoplastic process compared to those without recurrence (178.84; SD—12.98 vs. 205.66; SD—13.37). On the other hand, the level of serotonin before chemotherapy was higher in the group of people with disease recurrence compared to those without recurrence (141.53; SD—15.33 vs. 134.99; SD—10.08). Statistically significantly positive correlations were found between the level of β-endorphin and both disease-free time (β-endorphin levels before chemotherapy: rho Spearman 0.379, p < 0.027; β-endorphin levels after chemotherapy: rho Spearman 0.734 p < 0.001) and survival time (β-endorphin levels before chemotherapy: rho Spearman 0.267, p < 0.018; β-endorphin levels after chemotherapy: rho Spearman 0.654 p < 0.001). 1. The levels of serotonin and β-endorphin levels are significantly related to ovarian cancer and change during treatment. 2. High mean preoperative concentrations of β-endorphins were significantly related to overall survival and disease-free time.
Defeat, Entrapment, and Hopelessness: Clarifying Interrelationships between Suicidogenic Constructs
Authors: Oakey-Frost D. Nicolas , Moscardini Emma H. , Russell Kirsten , Rasmussen Susan , Cramer Robert J. , Tucker Raymond P.
Published: August 2022

Psychological theories of suicide posit conceptually similar constructs related to the development of suicidal thinking. These constructs often evince high-magnitude interrelationships across studies. Within these theories, defeat, entrapment and hopelessness standout as conceptually and quantitatively similar. Theoretical improvements may be facilitated through clarifying the subscale and item-level similarities among these constructs. Factor analytic and phenomenological work has demonstrated equivocal evidence for a distinction between defeat and entrapment; hopelessness is not typically analyzed together with defeat and entrapment despite evidence of large-magnitude interrelationships. This study explored the interrelationships among the foregoing constructs within a sample of undergraduate students (N = 344) from two universities within the Southeastern United States. Participants, oversampled for lifetime history of suicidal ideation and attempts, completed an online cross-sectional survey assessing defeat, entrapment, hopelessness and SI. Exploratory factor and parallel analyses demonstrated support for a one factor solution when analyzed at subscale level of the three measures as well as when all items of the three measures were analyzed together. Ad hoc exploratory structural equation modeling (ESEM) bifactor results evinced support for the existence of a single, general factor at the item level. Item level communalities and bifactor fit indices suggest that hopelessness may be somewhat distinct from defeat and entrapment. Clinical and theoretical implications are discussed in the context of study limitations.
Social Integration and Residence Intention of Foreigners in Western China: Evidence from Xi’an
Authors: Guo Xiaoxian , Zhang Qian , Cheng Yaxin , Song Jing’en
Published: August 2022

Since “Belt and Road Initiative” (BRI) of 2014, the number of foreigners in China has increased rapidly and China has become an importing country for immigrants, a change ongoing since the beginning of the 21st century. To respond to the rapidly increasing number of foreigners in China, the government frequently revised the immigration policies and issued new regulations for foreigners. However, scholars understand very little about how the foreigners perceive their integration into Chinese society or decide to pursue long-term residency or lawful permanent resident status. While some pioneering studies touch on this, with samples from the coastal megacities, no empirical evidence has been collected from smaller, inner cities. Three new findings about the foreigners in Xi’an, a major city in western China, fill this literature gap. First, the level of subjective social integration is largely influenced by the local networks. Second, the level of objective social integration depends largely on local and hometown networks. Third, the intention to obtain long-term and permanent residency in China is more evident in those foreigners who come from countries covered by the BRI and who consider China to be a better place to live than their home country.
Social, Economic and Overall Health Impacts of COVID-19 on People Living with Disabilities in King County, WA
Authors: Turcheti Nicole , Laurent Amy A. , Delgado Christina , Sainati Kayla , Johnson Kris , Wong Eva Y.
Published: August 2022

The COVID-19 pandemic and the associated mitigation measures to reduce the spread of disease affected the social, economic, and overall health of individuals. Quantitative administrative datasets typically did not contain demographic information that allowed for reporting or analysis of the impacts of COVID-19 on people living with disabilities. Understanding the experiences of this population during the pandemic can inform the design of public health responses that are more robust and better connected to community. This paper describes a qualitative participatory study with a diverse sample of people living with disabilities in King County, WA. Through 2 listening sessions and 35 semi-structured interviews, it examines what impacts COVID-19 brought for people living with disabilities; elucidates the supports that were helpful in addressing COVID-19 impacts; examines inequities faced by the disability community; and sheds light on how to engage with this community to inform the public health emergency response. The process, protocols, findings, and lessons learned are replicable by other local health departments and could be incorporated as part of routine data collection and considered for future public health emergencies.
Sickness Presenteeism among Employees Having Workplace Conflicts—Results from Pooled Analyses in Latvia
Authors: Lakiša Svetlana , Matisāne Linda , Gobiņa Inese , Orru Hans , Vanadziņš Ivars
Published: August 2022

The study’s objective was to investigate the associations between workplace conflicts and self-reported sickness presenteeism defined as going to work while being ill. Cross-sectional survey data pooled from four national surveys in years 2006, 2010, 2013 and 2018 with a study sample of 6368 employees (mean age 42.9 years and 52.9% females) were used. Respondents were randomly drawn from different regions and industries; therefore, the sample is representative of the working population of Latvia. The computer-assisted personal interviewing (CAPI) method was used to collect data at respondents’ places of residence. The associations between conflicts in the workplace and presenteeism were analyzed by using binomial logistic regression and calculated as odds ratios (ORs) with 95% confidence intervals (CIs) adjusted (aOR) for gender, age, education, and survey year. On average, 11% of respondents reported sickness presenteeism during the last year. The odds of presenteeism significantly increased for all types of workplace conflicts, but most for conflicts with managers (OR = 2.84). The odds of presenteeism doubled for those reporting conflicts with other employees (OR = 2.19) and conflicts with customers (OR = 1.85). The odds of sickness presenteeism were significantly higher if the workplace conflicts occurred often (seven times for conflicts between managers and employees, and four times for conflicts with customers) and with other employees. Presenteeism frequency increased more than three times if respondents had more than two types of conflict at work. The results of this study show that having any type of conflict in the workplace significantly increases the frequency of sickness presenteeism, especially when conflicts are frequent or an employee has more types of conflicts in the workplace. The study results justify the need to implement targeted and effective workplace conflict management measures at the organizational level to decrease sickness presenteeism.
Temporal Instability of Factors Affecting Injury Severity in Helmet-Wearing and Non-Helmet-Wearing Motorcycle Crashes: A Random Parameter Approach with Heterogeneity in Means and Variances
Authors: Ijaz Muhammad , Liu Lan , Almarhabi Yahya , Jamal Arshad , Usman Sheikh Muhammad , Zahid Muhammad
Published: August 2022

Not wearing a helmet, not properly strapping the helmet on, or wearing a substandard helmet increases the risk of fatalities and injuries in motorcycle crashes. This research examines the differences in motorcycle crash injury severity considering crashes involving the compliance with and defiance of helmet use by motorcycle riders and highlights the temporal variation in their impact. Three-year (2017–2019) motorcycle crash data were collected from RESCUE 1122, a provincial emergency response service for Rawalpindi, Pakistan. The available crash data include crash-specific information, vehicle, driver, spatial and temporal characteristics, roadway features, and traffic volume, which influence the motorcyclist’s injury severity. A random parameters logit model with heterogeneity in means and variances was evaluated to predict critical contributory factors in helmet-wearing and non-helmet-wearing motorcyclist crashes. Model estimates suggest significant variations in the impact of explanatory variables on motorcyclists’ injury severity in the case of compliance with and defiance of helmet use. For helmet-wearing motorcyclists, key factors significantly associated with increasingly severe injury and fatal injuries include young riders (below 20 years of age), female pillion riders, collisions with another motorcycle, large trucks, passenger car, drivers aged 50 years and above, and drivers being distracted while driving. In contrast, for non-helmet-wearing motorcyclists, the significant factors responsible for severe injuries and fatalities were distracted driving, the collision of two motorcycles, crashes at U-turns, weekday crashes, and drivers above 50 years of age. The impact of parameters that predict motorcyclist injury severity was found to vary dramatically over time, exhibiting statistically significant temporal instability. The results of this study can serve as potential motorcycle safety guidelines for all relevant stakeholders to improve the state of motorcycle safety in the country.
Self-Reported Sleepiness after 2, 4, and 7 Consecutive Night Shifts and Recovery Days in Danish Police Officers
Authors: Jensen Marie Aarrebo , Nielsen Helena Breth , Sallinen Mikael , Kristiansen Jesper , Hansen Åse Marie , Garde Anne Helene
Published: August 2022

Background: Night shift work often implies shorter sleep duration and this can lead to sleepiness, which has been associated with an increased risk of accidents and injuries. The aim is to study how the number of consecutive night shifts affects self-reported sleepiness. Participants and methods: The study was a quasi-experimental, within-subject crossover study with 73 police officers. Three work schedules of two, four, and seven consecutive night shifts followed by the same number of recovery days, i.e., days worked or days off, was performed by all participants. Sleepiness was self-reported using the Karolinska sleepiness scale (KSS) every fourth hour on the last night shift and the last recovery day in each sequence. Results: We observed differences in the level of sleepiness between recovery days and night shift days but no differences in the pattern of sleepiness levels on night shift days in the different work schedules. The highest levels of KSS were observed before bedtime (at 07:00 after a night shift and 23:00 on a recovery day). Conclusion: The number of consecutive night shifts did not affect the self-reported levels of self-reported sleepiness among Danish police officers.
Experiences of Health-Promoting Activities among Individuals with Knee Pain: The Halland Osteoarthritis Cohort
Authors: Sylwander Charlotte , Sunesson Evelina , Andersson Maria L. E. , Haglund Emma , Larsson Ingrid
Published: August 2022

Knee pain is an early sign of knee osteoarthritis (KOA) and a risk factor for chronic widespread pain (CWP). Early prevention is vital, and more research is needed to understand health-promoting activities for individuals with knee pain from a patient perspective. This study aimed to explore how individuals with knee pain experienced health-promoting activities. Explorative qualitative design with inductive approach was applied to explore the experiences of 22 individuals (13 women, 9 men; median age: 52). Semi-structured interviews were conducted and analysed using latent qualitative content analysis. The results revealed health-promoting activities in individuals with knee pain and were interpreted in the overall theme, striving for balance in everyday life. Two categories explored the content in health-promoting activities: (1) Caring for the body—being physically active, having a healthy diet, and utilising support; and (2) Managing life stressors—allowing for recovery, promoting vitality, and safeguarding healthy relationships. In conclusion, individuals with knee pain described various health-promoting activities. They strived for balance in everyday life by caring for the body and managing life stressors. We suggest that a broader approach to everyday life can be helpful in treatment plans and health promotion to manage and prevent KOA and CWP, while striving for a healthy lifestyle.
Relationship between Asymmetry Indices, Anthropometric Parameters, and Physical Fitness in Obese and Non-Obese High School Students
Authors: Haddad Monoem , Abbes Zied , Ibrahim Rony , Aganovic Zlatan , Bibi Khalid , Padulo Johnny
Published: August 2022

Impaired balance is associated with an increased risk of lower extremity injuries. The purpose of this study was to investigate the relationship between age, anthropometric measurement, and asymmetry index (AI) in an adolescent high school population. Twenty-six male students (15 ± 1.0 years) were randomly selected. Body composition, measurements of vertical jump height using a countermovement jump test (CMJ), and dynamic single stance balance using the Y-balance test (YBT), were collected over 4 weeks. Hierarchical multiple linear regression analyses were used as dimension reduction techniques in four different blocks to determine valid predictors for AIs. In the first regression analysis, controlling for age, body mass, height, and body mass index (BMI), the regression coefficient (B = 0.383, 95% confidence interval [CI] [0.088, 0.679], p < 0.05) associated with body fat indicated that with each additional unit of body fat, the YBT AI increased by 0.383 units. In the second regression analysis, controlling for age, body mass, and BMI, the regression coefficients associated with height (B = −1.692, 95% CI [−3.115, −0.269], p < 0.05] and body fat percentage (B = 0.529, 95% CI [0.075, 0.983], p < 0.05) indicated that with each additional unit of height or body fat percentage, the CMJ AI decreased by 1.692 units and increased by 0.529 units. Grouping participants based on body fat percentage had a significant effect on the AIs (p < 0.05) of the CMJ and YBT. The AI of the CMJ was 15% higher, and that of the YBT was 7% higher in non-obese students than obese students. These findings contribute to the knowledge of the local community and the emerging body of literature on motor skills and competence related to weight in this population.
Has Smartphone Use Influenced Loneliness during the COVID-19 Pandemic in Japan?
Authors: Nguyen Trinh Xuan Thi , Lal Sumeet , Abdul-Salam Sulemana , Yuktadatta Pattaphol , McKinnon Louis , Khan Mostafa Saidur Rahim , Kadoya Yoshihiko
Published: August 2022

The influence of smartphone use on increased risk of feeling lonely has been recognized as a global public health concern. However, it is unclear whether this influence has changed during the ongoing COVID-19 pandemic, during which smartphones have become a particularly important means of communication due to health safety measures restricting personal interactions. We used Hiroshima University’s online survey data collected from 18–28 February 2022, to assess the impact of smartphone use on loneliness in Japan. The final sample included 2630 participants aged over 20 years, with loneliness measured using the UCLA scale and smartphone use calculated as the duration of usage in minutes/day. Weighted logit regression analysis was used to examine the association between smartphone use and loneliness, with other demographic, socioeconomic, and psychological characteristics as explanatory variables. Contrary to conventional evidence, our findings show that smartphone use mitigated the risk of loneliness during the pandemic. This was especially true among females under 65 years old. We found that age, subjective health status, future anxiety, and depression impacted this relationship. The findings of this study can help guide policymaking by showing the importance of providing adequate digital platforms to manage loneliness and mental health during times of isolation.
Spatial Heterogeneity and Its Influencing Factors of Syphilis in Ningxia, Northwest China, from 2004 to 2017: A Spatial Analysis
Authors: Wang Ruonan , Li Xiaolong , Hu Zengyun , Jing Wenjun , Zhao Yu
Published: August 2022

Syphilis remains a growing and resurging infectious disease in China. However, exploring the influence of environmental factors on the spatiotemporal distribution of syphilis remains under explore. This study aims to analyze the spatiotemporal distribution characteristics of syphilis in Ningxia, Northwest China, and its potential environmental influencing factors. Based on the standardized incidence ratio of syphilis for 22 administrative areas in Ningxia from 2004 to 2017, spatiotemporal autocorrelation and scan analyses were employed to analyze the spatial and temporal distribution characteristics of syphilis incidence, while a fixed-effect spatial panel regression model identified the potential factors affecting syphilis incidence. Syphilis incidence increased from 3.78/100,000 in 2004 to 54.69/100,000 in 2017 with significant spatial clustering in 2007 and 2009–2013. The “high–high” and “low–low” clusters were mainly distributed in northern and southern Ningxia, respectively. The spatial error panel model demonstrated that the syphilis incidence may be positively correlated with the per capita GDP and tertiary industry GDP and negatively correlated with the number of health facilities and healthcare personnel. Sex ratio and meteorological factors were not significantly associated with syphilis incidence. These results show that the syphilis incidence in Ningxia is still increasing and has significant spatial distribution differences and clustering. Socio-economic and health-resource factors could affect the incidence; therefore, strengthening syphilis surveillance of migrants in the economically developed region and allocating health resources to economically underdeveloped areas may effectively help prevent and control syphilis outbreaks in high-risk cluster areas of Ningxia.
Effect of Small-Sided Games with and without the Offside Rule on Young Soccer Players: Reliability of Physiological Demands
Authors: Custódio Igor Junio Oliveira , Dos Santos Renan , de Oliveira Ildefonso Rafael , Andrade André , Diniz Rodrigo , Peixoto Gustavo , Bredt Sarah , Praça Gibson Moreira , Chagas Mauro Heleno
Published: August 2022

This study aimed to compare the physiological demand between three vs. three small-sided games (SSGs) with (3vs.3WITH) and without (3vs.3WITHOUT) the offside rule, as well as the within- and between-session reliability of this demand. Twenty-four U-17 soccer athletes performed various three vs. three (plus goalkeepers) SSGs with and without the offside rule. The data collection was performed within an eight-week period. Athletes’ heart rate was monitored during the SSG. The variables analyzed were the percentage mean heart rate (HRMEAN%) and the percentage peak heart rate (HRPEAK%). For the analysis of within-session reliability, the mean value of the first two and last two SSG bouts performed within one day were used. The between-session reliability was calculated using the mean value of the four SSG bouts of each SSG type performed on two different days. In both SSGs, the values for reliability were significant and were classified as moderate to excellent. There were no significant differences in the physiological demand among SSG types. We concluded that the offside rule does not influence the physiological demand in a three vs. three SSG and the HRMEAN% and HRPEAK% present moderate to excellent reliability in a three vs. three SSG with and without the offside rule.
Prolonged Grief Symptoms among Suicide-Loss Survivors: The Contribution of Intrapersonal and Interpersonal Characteristics
Authors: Levi-Belz Yossi , Ben-Yaish Tamir
Published: August 2022

Background: Suicide-loss survivors (SLSs) are a population with unique characteristics that place them at increased risk for developing grief complications and painful feelings of guilt that may impact their supportive social environment. However, no studies to date have examined the role of intrapersonal and interpersonal variables that may contribute to prolonged grief symptoms (PGS) as outlined by the new DSM-5 criteria. The present study aimed to extend knowledge regarding the role of interpersonal variables, such as perceived burdensomeness, thwarted belongingness, and self-disclosure, in determining the impact of guilt on the development of PGS among SLSs. Method: This study is part of a longitudinal study, though, in this study, we used a cross-sectional examination of the recently completed fourth measurement. Study participants included 152 SLSs aged 22 to 76 who completed questionnaires measuring guilt, depression, perceived burdensomeness, thwarted belongingness, self-disclosure, and PGS using the Prolonged Grief–Revised Inventory. Participants’ demographics and loss-related characteristics, such as time since suicide and participant’s age at the time of suicide, were examined. Results: Confirming the hypotheses, intrapersonal variables (i.e., guilt and depression), as well as interpersonal variables (i.e., perceived burdensomeness, thwarted belongingness, and self-disclosure), contributed significantly to PGS beyond sociodemographic and loss-related factors. Perceived burdensomeness significantly moderated the contribution of guilt to PGS: for participants with high burdensomeness levels, guilt contributed to PGS more strongly than for participants with low burdensomeness. Conclusion: Guilt is an important contributor to PGS among SLSs, and perceived burdensomeness plays a critical role in moderating this contribution. In light of these findings, it can be suggested that SLSs with high levels of guilt should receive special attention and may benefit from therapeutic interventions focusing on reducing maladaptive cognitions that elicit intense guilt or perceived burden.
Response Model for Urban Area Source Pollution and Water Environmental Quality in a River Network Region
Authors: Lai Qiuying , Ma Jie , He Fei , Wei Geng
Published: August 2022

With the development of cities, urban area source pollution has become more severe and a significant source of water pollution. To study the relationship between urban area source pollution and water environmental quality in a river network, this study uses a city in the Yangtze River Delta, China, as an example. The Storm Water Management Model (SWMM) model and the MIKE11 model were combined into a unified modeling framework and used to simulate dynamic changes in the water quality of a river network under light rain, moderate rain, and heavy rain. In the study period, the annual urban area source input loads of potassium permanganate (CODMn), total phosphorus (TP), and ammonia nitrogen were 29.8, 0.9, and 4.8 t, respectively. The influence of light rain on the water quality of the river network was lagging and temporary, and rainfall area pollution was the primary contributor. Under the scenario of moderate rain, overflow from a pipeline network compounded rainfall runoff, resulting in a longer duration of impact on the water quality in the river. Additionally, the water quality in the river course was worse under moderate rain than under light or heavy rain. Under the scenario of heavy rain, rain mainly served a dilutive function. This research can provide support for urban area source pollution control and management.
Talking on the Phone While Driving: A Literature Review on Driving Simulator Studies
Authors: Boboc Răzvan Gabriel , Voinea Gheorghe Daniel , Buzdugan Ioana-Diana , Antonya Csaba
Published: August 2022

Distracted driving is a growing concern around the world and has been the focus of many naturalistic and simulator-based studies. Driving simulators provide excellent practical and theoretical help in studying the driving process, and considerable efforts have been made to prove their validity. This research aimed to review relevant simulator-based studies focused on investigating the effects of the talking-on-the-phone-while-driving distraction on drivers’ behavior. This work is a scoping review which followed the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Extension for Scoping Reviews (PRISMA-ScR) guidelines. The search was performed on five databases, covering twenty years of research results. It was focused on finding answers to three research questions that could offer an overview of the main sources of distraction, the research infrastructure, and the measures that were used to analyze and predict the effects of distractions. A number of 4332 studies were identified in the database search, from which 83 were included in the review. The main findings revealed that TPWD distraction negatively affects driving performance, exposing drivers to dangerous traffic situations. Moreover, there is a general understanding that the driver’s cognitive, manual, visual, and auditory resources are all involved, to a certain degree, when executing a secondary task while driving.
Levels and Correlates of Numeracy Skills in Lebanese Adults with Diabetes: A Cross-Sectional Study
Authors: Bouclaous Carmel , Azar Luna Joe , Barmo Nour , Daher Ralph , Tabaja Jana , El Hout Ghida , Berika Lina
Published: August 2022

Diabetes numeracy skills are required in the interpretation of food labels, insulin pump dosage, the interpretation of blood glucose meter data, and the determination of carbohydrate intake. This study assessed the levels and correlates of numeracy skills in Lebanese adults with diabetes to identify those most at risk of uncontrolled diabetes. In total, 299 adults with diabetes, mean age 47.4 ± 19.8 years, took the questionnaire. It consisted of self-developed items on sociodemographic and health-related factors, in addition to the Diabetes Numeracy Test-15 (DNT-15) and the Single Item Literacy Screener. Many participants (62%) scored < 10 on the DNT-15 indicating insufficient numeracy skills. DNT-15 scores were positively associated with literacy, exercise, healthy diet, perceived diabetes control, frequency of glycaemia measurement, ability to afford treatment, and ease of understanding information related to diabetes. Age, BMI, and complications were negatively correlated with DNT-15 score. Numeracy skills were higher in males, single individuals, and in people with type 1 diabetes, fewer complications, controlled HbA1c, higher income, higher education, a prior visit to a dietician, and ability to maintain personal care despite COVID-19. Interventions to strengthen numeracy skills would empower individuals with diabetes, lead to appropriate self-management behaviors, and prevent health complications in at-risk individuals.
Emotional Burden among Pharmacists and Pharmacy Technicians during the COVID-19 Lockdown: A Cross Sectional Study
Authors: Baldonedo-Mosteiro Carmen , Mosteiro-Díaz María-Pilar , Franco-Correia Sara , Tardón Adonina
Published: August 2022

This study aims to investigate the prevalence of depression and anxiety symptoms among Spanish community pharmacists and pharmacy technicians during the coronavirus disease 2019 (COVID-19) lockdown. A descriptive cross-sectional quantitative study was designed. An online survey was administered to participants from 4 to 21 April 2020 using a questionnaire assessing sociodemographic information and the Spanish version of the Hospital Anxiety and Depression Scale (HADS). Informed consent to participate was requested. Participants comprised 1162 pharmacy staff from Spain with an average age of 39.15 ± 9.718, from 20 to 65 years old, of whom 83% were women, and 50.6% were married. More than half of the participants expressed symptoms of depression (62.7%) and anxiety (70.9%). An important prevalence of anxiety and depression symptoms has been detected among Spanish pharmacists and pharmacy technicians during the COVID-19 lockdown. Being a woman, smoking, feeling fear, feeling stress and believing that pharmacists/pharmacy technicians are very exposed to COVID-19 seem to be associated with higher HADS scores.
Gender Differences in Associations between Biomechanical and Psychosocial Work Exposures and Age of Withdrawal from Paid Employment among Older Workers
Authors: Undem Karina , Leinonen Taina , Kristensen Petter , Merkus Suzanne L. , Hasting Rachel L. , Gran Jon Michael , Mehlum Ingrid S.
Published: August 2022

Background: Work exposures are known predictors of withdrawal from employment, but the associations between work exposures and withdrawal may vary with gender. This study evaluated gender differences in associations between biomechanical and psychosocial work exposures and age of withdrawal from paid employment among older workers in Norway. Methods: 77,558 men and 67,773 women (born 1949–1953) were followed from age 62 until withdrawal from paid employment or end of follow-up in 2016 (up to five years follow-up). Information about eight biomechanical and seven psychosocial exposures was obtained from a gender-specific job exposure matrix. Using Cox regression, the difference in mean estimated time until withdrawal between non-exposed and exposed was calculated for each gender and work exposure separately. Results: The largest gender difference was found for high psychological demands. Among men, the non-exposed withdrew earlier than the exposed (−3.66 months (95% CI: −4.04–−3.25 months)), and contrary among women (0.71 (0.28–1.10)), resulting in a gender difference of 4.37 (3.81–4.97) months. Gender differences were also found for monotonous work (4.12 (3.51–4.69) months), hands above shoulder height (2.41 (1.76–3.10) months), and high iso-strain (2.14 (1.38–2.95) months). Conclusions: There were observed gender differences in the associations between some biomechanical and psychosocial work exposures and mean age of withdrawal from paid employment among older workers. However, the results are likely affected by the selection of who remains in the workforce at age 62 and should be interpreted accordingly.
Colorimetric Approach for Nucleic Acid Salmonella spp. Detection: A Systematic Review
Authors: Ahmad Faris Asma Nadia , Ahmad Najib Mohamad , Mohd Nazri Muhammad Najmi , Hamzah Amir Syahir Amir , Aziah Ismail , Yusof Nik Yusnoraini , Mohamud Rohimah , Ismail Irneza , Mustafa Fatin Hamimi
Published: August 2022

Water- and food-related health issues have received a lot of attention recently because food-poisoning bacteria, in particular, are becoming serious threats to human health. Currently, techniques used to detect these bacteria are time-consuming and laborious. To overcome these challenges, the colorimetric strategy is attractive because it provides simple, rapid and accurate sensing for the detection of Salmonella spp. bacteria. The aim of this study is to review the progress regarding the colorimetric method of nucleic acid for Salmonella detection. A literature search was conducted using three databases (PubMed, Scopus and ScienceDirect). Of the 88 studies identified in our search, 15 were included for further analysis. Salmonella bacteria from different species, such as S. Typhimurium, S. Enteritidis, S. Typhi and S. Paratyphi A, were identified using the colorimetric method. The limit of detection (LoD) was evaluated in two types of concentrations, which were colony-forming unit (CFU) and CFU per mL. The majority of the studies used spiked samples (53%) rather than real samples (33%) to determine the LoDs. More research is needed to assess the sensitivity and specificity of colorimetric nucleic acid in bacterial detection, as well as its potential use in routine diagnosis.