bluuebunny/crossref_metadata_2025 huggingface dataset:
HIV-1 and IL-1β regulate astrocytic CD38 through mitogen-activated protein kinases and nuclear factor-κB signaling mechanisms
Authors: Mamik Manmeet K , Banerjee Sugato , Walseth Timothy F , Hirte Renee , Tang Lin , Borgmann Kathleen , Ghorpade Anuja
Published: December 2011

Background

Infection with human immunodeficiency virus type-1 (HIV)-1 leads to some form of HIV-1-associated neurocognitive disorders (HAND) in approximately half of the cases. The mechanisms by which astrocytes contribute to HIV-1-associated dementia (HAD), the most severe form of HAND, still remain unresolved. HIV-1-encephalitis (HIVE), a pathological correlate of HAD, affects an estimated 9-11% of the HIV-1-infected population. Our laboratory has previously demonstrated that HIVE brain tissues show significant upregulation of CD38, an enzyme involved in calcium signaling, in astrocytes. We also reported an increase in CD38 expression in interleukin (IL)-1β-activated astrocytes. In the present investigation, we studied regulatory mechanisms of CD38 gene expression in astrocytes activated with HIV-1-relevant stimuli. We also investigated the role of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB in astrocyte CD38 regulation.

Methods

Cultured human astrocytes were transfected with HIV-1YU-2proviral clone and levels of CD38 mRNA and protein were measured by real-time PCR gene expression assay, western blot analysis and immunostaining. Astrocyte activation by viral transfection was determined by analyzing proinflammatory chemokine levels using ELISA. To evaluate the roles of MAPKs and NF-κB in CD38 regulation, astrocytes were treated with MAPK inhibitors (SB203580, SP600125, U0126), NF-κB interfering peptide (SN50) or transfected with dominant negative IκBα mutant (IκBαM) prior to IL-1β activation. CD38 gene expression and CD38 ADP-ribosyl cyclase activity assays were performed to analyze alterations in CD38 levels and function, respectively.

Results

HIV-1YU-2-transfection significantly increased CD38 mRNA and protein expression in astrocytes (p < 0.01) in a dose-dependent manner and induced astrocyte activation. IL-β-activation of HIV-1YU-2-transfected astrocytes significantly increased HIV-1 gene expression (p < 0.001). Treatment with MAPK inhibitors or NF-κB inhibitor SN50 abrogated IL-1β-induced CD38 expression and activity in astrocytes without altering basal CD38 levels (p < 0.001). IκBαM transfection also significantly inhibited IL-1β-mediated increases in CD38 expression and activity in astrocytes (p < 0.001).

Conclusion

The present findings demonstrate a direct involvement of HIV-1 and virus-induced proinflammatory stimuli in regulating astrocyte-CD38 levels. HIV-1YU-2-transfection effectively induced HIV-1p24 protein expression and activated astrocytes to upregulate CCL2, CXCL8 and CD38. In astrocytes, IL-1β-induced increases in CD38 levels were regulated through the MAPK signaling pathway and by the transcription factor NF-κB. Future studies may be directed towards understanding the role of CD38 in response to infection and thus its role in HAND.
Effects of Sound on Postural Stability during Quiet Standing
Authors: Park Sung Ha , Lee Kichol , Lockhart Thurmon , Kim Sukwon
Published: December 2011

Loss of postural stability can increase the likelihood of slips and falls in workplaces. The present study intended to extend understanding of the effects of frequency and pressure level of sound on postural stability during standing. Eleven male subjects participated. Standing on a force platform, the subjects' center of pressures were measured under different combinations of pressure level and frequency of the sound. Variables such as the position variability of COP and the length of postural sway path in anterior-posterior (AP) and medio-lateral (ML) direction were evaluated. Subjective ratings of perceived disturbance at each experimental condition were also obtained using a 7-point rating scale. Results showed that the length of sway path and the position variability of COP increased as the frequency of sound increased in posterior-anterior axis. The effect of sound pressure level, however, was not significant on both the postural sway length and the position variability of COP. These results suggested substantial disturbance of standing balance system among subjects exposed to high frequency noise. The results implied that physical workers should be alerted that their abilities of postural balance could be degraded significantly as disturbance caused by a sound existed.
Prevalence of porcine circovirus-like agent P1 in Jiangsu, China
Authors: Wen Libin , He Kongwang , Yang Hanchun , Yu Zhengyu , Mao Aihua , Zhong Shulin , Ni Yanxiu , Zhang Xuehan , Li Bin , Wang Xiaomin , Zhou Junming , Guo Rongli , Lv Lixin , Jiang Jieyuan
Published: December 2011

Recently, we identified a novel porcine circovirus type 2-like agent P1 isolate from swine. The present study represents the first survey of P1 prevalence in swine herds from Jiangsu, China, by using PCR targeting the complete genome of P1. Prevalences of 50% and 19% were found among 6 herds and 248 animals, respectively. The results indicate a high prevalence of P1 in China pig populations.
Histopathological investigation in porcine infected with torque teno sus virus type 2 by inoculation
Authors: Mei Miao , Zhu Ling , Wang Yun , Xu Zhiwen , Zhao Ling , Peng Xi , Wu Yunfei , Li Song , Guo Wanzhu
Published: December 2011

Background

Porcine torque teno sus virus (TTSuV) is a small icosahedral and non-enveloped virus which contains a single-stranded (ssDNA), circular and negative DNA genome and infects mainly vertebrates and is currently classified into the 'floating' genus Anellovirus of Circoviridae with two species. Viral DNA of both porcine TTSuV species has a high prevalence in both healthy and diseased pigs worldwide and multiple infections of TTSuV with distinct genotypes or subtypes of the same species has been documented in the United States, Europe and Asia. However, there exists no information about histopathological lesions caused by infection with porcine TTSuV2.

Methods

Porcine liver tissue homogenate with 1 ml of 6.91 × 107genomic copies viral loads of porcine TTSuV2 that had positive result for torque teno sus virus type 2 and negative result for torque teno sus virus type 1 and porcine pseudorabies virus type 2 were used to inoculate specific pathogen-free piglets by intramuscular route and humanely killed at 3,7,10,14,17,21 and 24 days post inoculation (dpi), the control pigs were injected intramuscularly with 1 ml of sterile DMEM and humanely killed the end of the study for histopathological examination routinely processed, respectively.

Results

All porcine TTSuV2 inoculated piglets were clinic asymptomatic but developed myocardial fibroklasts and endocardium, interstitial pneumonia, membranous glomerular nephropathy, and modest inflammatory cells infiltration in portal areas in the liver, foci of hemorrhage in some pancreas islet, a tiny amount red blood cells in venule of muscularis mucosae and outer longitudinal muscle, rarely red blood cells in the microvasculation and infiltration of inflammatory cells (lymphocytes and eosinophils) of tonsil and hilar lymph nodes, infiltration of inflammatory lymphocytes and necrosis or degeneration and focal gliosis of lymphocytes in the paracortical zone after inoculation with porcine TTSuV2-containing tissue homogenate.

Conclusions

Analysis of these presentations revealed that porcine TTSuV2 was readily transmitted to TTSuV-negative swine and that infection was associated with characteristic pathologic changes in specific pathogen-free piglets inoculated with porcine TTSuV2. Those results indicated no markedly histopathological changes happened in those parenchymatous organs, especially the digestive system and immune system when the specific pathogen-free pigs were infected with porcine TTSuV2, hence, to some extent, it was not remarkable pathological agent for domestic pigs at least. So, porcine TTSuV2 could be an unrecognized pathogenic viral infectious etiology of swine. This study indicated a directly related description of lesions responsible for TTSuV2 infection in swine.
Reproducible computational biology experiments with SED-ML - The Simulation Experiment Description Markup Language
Authors: Waltemath Dagmar , Adams Richard , Bergmann Frank T , Hucka Michael , Kolpakov Fedor , Miller Andrew K , Moraru Ion I , Nickerson David , Sahle Sven , Snoep Jacky L , Le Novère Nicolas
Published: December 2011

Background

The increasing use of computational simulation experiments to inform modern biological research creates new challenges to annotate, archive, share and reproduce such experiments. The recently published Minimum Information About a Simulation Experiment (MIASE) proposes a minimal set of information that should be provided to allow the reproduction of simulation experiments among users and software tools.

Results

In this article, we present the Simulation Experiment Description Markup Language (SED-ML). SED-ML encodes in a computer-readable exchange format the information required by MIASE to enable reproduction of simulation experiments. It has been developed as a community project and it is defined in a detailed technical specification and additionally provides an XML schema. The version of SED-ML described in this publication is Level 1 Version 1. It covers the description of the most frequent type of simulation experiments in the area, namely time course simulations. SED-ML documents specify which models to use in an experiment, modifications to apply on the models before using them, which simulation procedures to run on each model, what analysis results to output, and how the results should be presented. These descriptions are independent of the underlying model implementation. SED-ML is a software-independent format for encoding the description of simulation experiments; it is not specific to particular simulation tools. Here, we demonstrate that with the growing software support for SED-ML we can effectively exchange executable simulation descriptions.

Conclusions

With SED-ML, software can exchange simulation experiment descriptions, enabling the validation and reuse of simulation experiments in different tools. Authors of papers reporting simulation experiments can make their simulation protocols available for other scientists to reproduce the results. Because SED-ML is agnostic about exact modeling language(s) used, experiments covering models from different fields of research can be accurately described and combined.
Role of blood cell-associated angiotensin II type 1 receptors in the cerebral microvascular response to ischemic stroke during angiotensin-induced hypertension
Authors: Nagai Mutsumi , Terao Satoshi , Vital Shantel A , Rodrigues Stephen F , Yilmaz Gokhan , Granger D Neil
Published: December 2011

Background

Angiotensin II type 1 receptor (AT1R) blockers lower the incidence of ischemic stroke in hypertensive patients and attenuate brain inflammation and injury in animal models. Although AT1R on both blood cells (BC) and vascular endothelial cells (EC) can be activated by angiotensin II (Ang II) to elicit inflammation, little is known about the relative contributions of AT1R expressed on BC and EC to the brain injury responses to ischemia and reperfusion (I/R) in the setting of angiotensin-induced hypertension.

Methods

The contributions of BC- and EC-associated AT1R to I/R-induced brain inflammation and injury were evaluated using wild type (WT), AT1aR-/-, and bone marrow chimera mice with either a BC+/EC+ (WT→WT) or BC-/EC+ (AT1aR-/-→WT) distribution of AT1aR. The adhesion of leukocytes and platelets in venules, blood brain barrier (BBB) permeability and infarct volume were monitored in postischemic brain of normotensive and Ang II-induced hypertensive mice.

Results

The inflammatory (blood cell adhesion) and injury (BBB permeability, infarct volume) responses were greatly exaggerated in the presence of Ang II-induced hypertension. The Ang II-enhanced responses were significantly blunted in AT1aR-/-mice. A similar level of protection was noted in AT1aR-/-→WT mice for BBB permeability and infarct volume, while less or no protection was evident for leukocyte and platelet adhesion, respectively.

Conclusions

BC- and EC-associated AT1aR are both involved in the brain injury responses to ischemic stroke during Ang II-hypertension, with EC AT1aR contributing more to the blood cell recruitment response and BC AT1aR exerting a significant influence on the BBB disruption and tissue necrosis elicited by I/R.
Management of people with acute low-back pain: a survey of Australian chiropractors
Authors: Walker Bruce F , French Simon D , Page Matthew J , O'Connor Denise A , McKenzie Joanne E , Beringer Katherine , Murphy Kerry , Keating Jenny L , Michie Susan , Francis Jill J , Green Sally E
Published: December 2011

Introduction

Chiropractors commonly provide care to people with acute low-back pain (LBP). The aim of this survey was to determine how chiropractors intend to support and manage people with acute LBP and if this management is in accordance with two recommendations from an Australian evidence-based acute LBP guideline. The two recommendations were directed at minimising the use of plain x-ray and encouraging the patient to stay active.

Methods

This is a cross sectional survey of chiropractors in Australia. This paper is part of the ALIGN study in which a targeted implementation strategy was developed to improve the management of acute LBP in a chiropractic setting. This implementation strategy was subsequently tested in a cluster randomised controlled trial. In this survey phase of the ALIGN study we approached a random sample of 880 chiropractors in three States of Australia. The mailed questionnaire consisted of five patient vignettes designed to represent people who would typically present to chiropractors with acute LBP. Four vignettes represented people who, according to the guideline, would not require a plain lumbar x-ray, and one vignette represented a person with a suspected vertebral fracture. Respondents were asked, for each vignette, to indicate which investigation(s) they would order, and which intervention(s) they would recommend or undertake.

Results

Of the 880 chiropractors approached, 137 were deemed ineligible to participate, mostly because they were not currently practising, or mail was returned to sender. We received completed questionnaires from 274 chiropractors (response rate of 37%). Male chiropractors made up 66% of respondents, 75% practised in an urban location and their mean number of years in practice was 15. Across the four vignettes where an x-ray was not indicated 68% (95% Confidence Intervals (CI): 64%, 71%) of chiropractors responded that they would order or take an x-ray. In addition 51% (95%CI: 47%, 56%) indicated they would give advice to stay active when it was indicated. For the vignette where a fracture was suspected, 95% (95% CI: 91%, 97%) of chiropractors would order an x-ray.

Conclusion

The intention of chiropractors surveyed in this study shows low adherence to two recommendations from an evidence-based guideline for acute LBP. Quality of care for these patients could be improved through effective implementation of evidence-based guidelines. Further research to find cost-effective methods to increase implementation is warranted.
Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry
Authors: Nagaveni Voleti , Sravani Manyam , Prabhakar Sripadi , Vairamani Mariappanadar
Published: December 2011

Protein–ligand interactions were studied for bovine insulin–amino sugar systems under electrospray ionisation mass spectrometry conditions. The isomeric amino sugars showed differences in the relative abundance of 1:1 protein–ligand complex formation. The electrospray ionisation and tandem mass spectrometry results of the complex clearly demonstrated that the differences in the interaction of isomeric sugars with insulin are mainly due to the differences in their gas-phase basicity. The same phenomenon is replicated in the formation of complexes between insulin and other ligands, such as amino acids, as well as in the binding of the amino sugars with amyloid β 1–40 peptide.
Launching nurse-initiated HIV rapid testing in Veterans Affairs primary care: a comprehensive overview of a self-sustaining implementation
Authors: Knapp H , Anaya H D , Feld J E , Hoang T , Goetz M B
Published: December 2011

Our objectives were to use foundational pilot findings to guide the implementation of an HIV rapid testing (RT) intervention at one Veterans Affairs outpatient clinic and to evaluate the success and sustainability of this intervention over the course of one year. Policy modifications were drafted and adopted to enable nurses to order, administer, interpret and document HIV RTs. Staff enrolled in a two-part training sessions designed to teach pre- and post-test counselling techniques and the mechanics of administering, interpreting and coding test results in the patients’ medical records. They were subsequently evaluated on their efforts at: (1) increasing HIV RT, (2) sustaining this effort one year post-launch. Enabling nurses to carry out HIV RT resulted in a significant increase in not only HIV RT, but also HIV testing rates overall at this facility, measured over the first year of this implementation. Our findings indicate that targeted strategies, aimed at increasing HIV RT rates, worked to increase testing rates overall, and also, that our initial testing strategies were independently sustainable, which is in contrast to findings in the literature on implementation science.
Severe Neutropenia Following a Prolonged Course of Vancomycin That Progressed to Agranulocytosis with Drug Reexposure
Authors: Duff Jennifer M , Moreb Jan S , Muwalla Firas
Published: December 2011

OBJECTIVE:

To report a case of severe neutropenia after discontinuing prolonged treatment with vancomycin that progressed to agranulocytosis with vancomycin reexposure.

CASE SUMMARY:

A 78-year-old woman presented with severe neutropenia (absolute neutrophil count [ANC] 37 cells/μL) and hypocellular bone marrow with absence of myeloid elements 8 weeks after discontinuing a 3-week treatment course of vancomycin 750 mg every 12 hours. Filgrastim 300 μg daily was started for neutropenia and vancomycin 750 mg every 12 hours and aztreonam 1 g every 8 hours were initiated for catheter-related acute thrombophlebitis of the upper extremity. The patient's ANC decreased to 10 cells/μL within 3 days of starting vancomycin. We suspected an autoimmune process, potentially related to vancomycin exposure, and began treatment with methylprednisolone 1 mg/kg daily. The ANC precipitously dropped to 0 cells/μL despite treatment with steroids and an increased filgrastim dose of 480 μg/day. All antibiotics were discontinued on the fifth day of hospitalization. Within 48 hours, her neutrophil count showed recovery (white blood cell count 500 cells/μL; 10% neutrophils).

DISCUSSION:

Idiosyncratic drug-induced agranulocytosis is an uncommon phenomenon but is often associated with serious consequences such as sepsis. We believe this case is unique because of the unusually late neutropenia discovered several weeks after finishing a prolonged course of vancomycin. Furthermore, agranulocytosis developed after unintentional rechallenge with vancomycin. According to the Naranjo probability scale, this case illustrates a probable adverse event caused by vancomycin.

CONCLUSIONS:

This case demonstrates a serious adverse event potentially associated with vancomycin use, and calls attention to the safety of rechallenging with vancomycin during a possible drug-induced neutropenia.
Thyroid hyperactivity with high thyroglobulin in serum despite sufficient iodine intake in chronic cold adaptation in an Arctic Inuit hunter population
Authors: Andersen Stig , Kleinschmidt Kent , Hvingel Bodil , Laurberg Peter
Published: December 2011

Objective

Adult man hosts brown adipose tissue with the capacity to consume energy and dissipate heat. This is essential for non-shivering thermogenesis and its activation depends on sympathetic activity and thyroid hormones. This led us to evaluate the impact of chronic cold exposure on thyroid activity and thyroid hormones in serum in Arctic residents.

Design

Comparative, population-based study (n=535) performed in Greenland.

Methods

Hunters were compared with other men, and Inuit in remote settlements in East Greenland with no modern housing facilities were compared with the residents of the capital city in West Greenland and residents of a major town in East Greenland in a cross-sectional study. We used interview-based questionnaires, measured TSH, free thyroxine, free triiodothyronine (fT3), thyroglobulin (TG) antibody and TG (a measure of thyroid activity) in serum, and iodine and creatinine in spot urine samples.

Results

Serum TG was the highest among hunters (P=0.009) and settlement dwellers (P=0.001), who were most markedly exposed to cold, even though they had the highest urinary iodine excretion (hunters,P<0.001; settlement dwellers,P<0.001). Hunters and settlement dwellers also had the lowest fT3(hunters,P<0.001; settlement dwellers,P<0.001) after adjusting for gender, age, smoking habits, alcohol intake and iodine excretion in multivariate linear regression models. TSH was not influenced by measures of cold exposure (hunter,P=0.36; residence,P=0.91).

Conclusions

Cold exposure influenced thyroid hormones and TG in serum in Arctic populations consistent with consumption of thyroid hormone and higher thyroid hormone turnover. Findings emphasise that changes in thyroid activity are essential in cold adaptation in Arctic residents.
Age dependent changes in arsenic and nicotine induced oxidative stress in male rat
Authors: Jain Anshu , Flora Swaran J. S.
Published: December 2011

The present study was planned to compare the differential toxicity of arsenic and nicotine among three different age groups of rats. In this study blood and tissue oxidative stress was determined in different age group of rats exposed to arsenic (2 mg/kg, i.p.) and nicotine (6 mg/kg, i.p.) for 2 weeks. Arsenic inhibited blood δ-aminolevulinic acid dehydratase activity in all age groups of rats while, a moderate inhibition was noted following nicotine exposure in young and old rats. Arsenic caused an increase in blood reactive oxygen species in young and old rats while glutathione level increased only in young rats unlike nicotine. A significant increase in thiobarbituric acid reactive substance was noted in liver of young and old rats while, hepatic GSH showed an increase in young and a decrease in old rats. Catalase activity decreased significantly in arsenic exposed young and old rats. At the given dose and duration both toxicants did not induce oxidative stress in rat brain. Blood arsenic concentration increased with age whereas its level brain and liver was independent of age. Conclusively, young and old animals were found to be most sensitive to both toxicants; with arsenic being more toxic than nicotine targeting liver.
Impact of anesthesia on patient and endoscopist satisfaction after colonoscopy — A pilot study
Authors: Szegô Eszter , Iványi Zsolt , László András , Gál János
Published: December 2011

Introduction

Colonoscopy is a standard diagnostic tool for the investigation and surveillance of diseases affecting the colon. The procedure can be uncomfortable and sometimes very painful, resulting in increased cecal intubation time and lower completion rate. However, it seems to be apparent that anesthesia for this procedure increases patient satisfaction; data are lacking about the impact of anesthesia on the technical performance of colonoscopic examination.

Aim

In our observational survey, we studied patients undergoing colonoscopy with or without anesthesia. We compared patient satisfaction, difficulties in endoscopy, and the impact of anesthesia on the examination room occupancy.

Methods

We enrolled 60 patients undergoing elective, outpatient colonoscopy because of various reasons. The patients were able to choose between anesthesia and sedation. Difficulties in colonoscopy were evaluated by the endoscopist's rating and by the time to cecal intubation. We assessed patient satisfaction by a numeric rating scale.

Results

We observed that neither the duration of colonoscopy nor the time spent in the examination room was different in the two groups (p 0.825, 0.998). There was a significant improvement in both patient and endoscopist satisfaction scores in patients undergoing anesthesia (p 0.0007).

Conclusion

We found that during colonoscopy, compared to sedation, anesthesia increases both endoscopist and patient satisfaction without prolonged occupation of the examination room.
New possibilities in the endovascular treatment of supraaortic vessels
Authors: Nemes Balázs
Published: December 2011

Cerebrovascular disease, including stroke, represents the third-leading cause of death in Hungary and a leading cause of disability among the elderly population. The majority of all strokes are ischemic, mostly secondary to thromboembolic disease of the supraaortic vessels. We investigated new therapeutic methods in the endovascular treatment of these diseases. Surgical revascularization of supraaortic trunk stenosis is associated with high morbidity and mortality rates. Balloon angioplasty has become an increasingly accepted treatment of stenoocclusive supraaortic arterial disease. Natural history data and treatment guidelines do not exist for innominate and proximal common carotid artery lesions. We have confirmed in a large series of innominate artery angioplasties that it is a safe and effective procedure with an excellent initial success rate, with a lower complication rate than the surgical option and with a similar long-term patency rate as for surgery.

In the largest published study on transfemoral angioplasty of ostial and proximal common carotid artery stenosis we have proved that endovascular treatment has high success rate with low stroke/death rate. Carotid stenting (CAS) is an evolving alternative to surgery in the treatment of patients with carotid stenosis. Stent selection is influenced by several factors, including the carotid anatomy and lesion characteristics. We examined the wall adaptability of a new closed-cell carotid stent (NexStent), which was designed for carotid bifurcation treatment. Data obtained from angiographic and computed tomographic images indicate that the stent provides adequate expansion and adaptation to the carotid bifurcation.

There are two types of restenosis after carotid artery interventions: the early restenosis develops mainly within the first 24 months after the revascularization procedure and its pathological background is myointimal hyperplasia; on the other hand late restenosis is rather due to progression of primary atherosclerosis and occurs more than 2 years after carotid endarterectomy (CEA). We compared the early restenosis rate in a consecutive series of CAS versus CEA patients at a single cardiovascular institution. The data suggest that the incidence of restenosis after stenting was less common than after surgery.

Our results may help vascular surgeons and interventional radiologists to consider risk versus benefit when deciding treatment options for supraaortic arterial stenosis.
Decision support for the management of oil well drill cuttings
Authors: Abbe Owen E. , Grimes Sue M. , Fowler Geoff D.
Published: December 2011

Depending on the hole size and mud type used in the drilling process, oil well drill cuttings can be a relatively high-volume solid waste stream from drilling operations in the oil and gas exploration and production industry. Current management practices tend to involve thermal treatment followed by landfill disposal. The waste-to-resource conversion approach, however, provides opportunities that are not reflected in the current management system. The drill cuttings waste management decision support tool described in this work allows for the consideration of alternative reuse applications such as in construction materials, oil well reinjection, wetlands restoration, and the manufacture of stable leach-resistant material such as glass ceramics, in a move to divert treated drill cuttings from landfill towards zero waste disposal.
A streamlined life-cycle assessment and decision tool for used tyres recycling
Authors: Curry Robin , Powell Jane , Gribble Nicola , Waite Steve
Published: December 2011

In order to achieve progress towards sustainable resource management, it is essential to evaluate options for the reuse and recycling of secondary raw materials, in order to provide a robust evidence base for decision makers. This paper presents the research undertaken in the development of a web-based decision-support tool (the used tyres resource efficiency tool) to compare three processing routes for used tyres compared to their existing primary alternatives. Primary data on the energy and material flows for the three routes, and their alternatives were collected and analysed. The methodology used was a streamlined life-cycle assessment (sLCA) approach. Processes included were: car tyre baling against aggregate gabions; car tyre retreading against new car tyres; and car tyre shred used in landfill engineering against primary aggregates. The outputs of the assessment, and web-based tool, were estimates of raw materials used, carbon dioxide emissions and costs. The paper discusses the benefits of carrying out a streamlined LCA and using the outputs of this analysis to develop a decision-support tool. The strengths and weakness of this approach are discussed and future research priorities identified which could facilitate the use of life cycle approaches by designers and practitioners.
Pollution from waste in Archangelsk region, north-west Russia
Authors: Román Elisabeth , Hiljanen Risto
Published: December 2011

The first list identifying environmental ‘hot spots’ in the Russian part of the Barents region was prepared in 1995. An updated report, prepared by the Nordic Environment Finance Corporation in collaboration with the Arctic Monitoring and Assessment Programme in 2003, lists 42 hot spots and proposals for 52 investment projects to mitigate the environmental impact of these hot spots, which are given priority based on appreciation of the impacts they have on ecosystems and population. The Barents Hot Spots Facility (BHSF) programme, which receives funding from the governments of Finland, Iceland, Norway and Sweden, aims to reduce the environmental problems described in the hot spots list. The nuclear safety sector is not included. From October 2007 to February 2008, a project concerning pollution abatement opportunities in the waste management sector in the Archangelsk region was carried out within the BHSF programme. The objective of the project was to analyse toxic solid waste in the hot spot of Archangelsk Oblast and make recommendations for technical assistance and possible BHSF funding. In this article, the situation in the Archangelsk region is described and conclusions are drawn for the actions needed.
Reduced pollinator service and elevated pollen limitation at the geographic range limit of an annual plant
Authors: Moeller David A. , Geber Monica A. , Eckhart Vincent M. , Tiffin Peter
Published: December 2011

Mutualisms are well known to influence individual fitness and the population dynamics of partner species, but little is known about whether they influence species distributions and the location of geographic range limits. Here, we examine the contribution of plant–pollinator interactions to the geographic range limit of the California endemic plant Clarkia xantiana ssp. xantiana. We show that pollinator availability declined from the center to the margin of the geographic range consistently across four years of study. This decline in pollinator availability was caused to a greater extent by variation in the abundance of generalist rather than specialist bee pollinators. Climate data suggest that patterns of precipitation in the current and previous year drove variation in bee abundance because of its effects on cues for bee emergence in the current year and the abundance of floral resources in the previous year. Experimental floral manipulations showed that marginal populations had greater outcross pollen limitation of reproduction, in parallel with the decline in pollinator abundance. Although plants are self‐compatible, we found no evidence that autonomous selfing contributes to reproduction, and thus no evidence that it alleviates outcross pollen limitation in marginal populations. Furthermore, we found no association between the distance to the range edge and selfing rate, as estimated from sequence and microsatellite variation, indicating that the mating system has not evolved in response to the pollination environment at the range periphery. Overall, our results suggest that dependence on pollinators for reproduction may be an important constraint limiting range expansion in this system.
The Impact of the Individual Mandate and Internal Revenue Service Form 990 Schedule H on Community Benefits From Nonprofit Hospitals
Authors: Principe Kristine , Adams E. Kathleen , Maynard Jenifer , Becker Edmund R.
Published: December 2011

In response to a growing concern that nonprofit hospitals are not providing sufficient benefit to their communities in return for their tax-exempt status, the Internal Revenue Service (IRS) now requires nonprofit hospitals to formally document the extent of their community contributions.

While the IRS is increasing financial scrutiny of nonprofit hospitals, many provisions in the recently passed historical health reform legislation will also have a significant impact on the provision of uncompensated care and other community benefits.

We argue that health reform does not render the nonprofit organizational form obsolete. Rather, health reform should strengthen the nonprofit hospitals’ ability to fulfill their missions by better targeting subsidies for uncompensated care and potentially increasing subsidized health services provision, many of which affect the public's health.
Individual Participant Data Meta-Analysis of Mechanical Workplace Risk Factors and Low Back Pain
Authors: Griffith Lauren E. , Shannon Harry S. , Wells Richard P. , Walter Stephen D. , Cole Donald C. , Côté Pierre , Frank John , Hogg-Johnson Sheilah , Langlois Lacey E.
Published: December 2011

Objectives. We used individual participant data from multiple studies to conduct a comprehensive meta-analysis of mechanical exposures in the workplace and low back pain.

Methods. We conducted a systematic literature search and contacted an author of each study to request their individual participant data. Because outcome definitions and exposure measures were not uniform across studies, we conducted 2 substudies: (1) to identify sets of outcome definitions that could be combined in a meta-analysis and (2) to develop methods to translate mechanical exposure onto a common metric. We used generalized estimating equation regression to analyze the data.

Results. The odds ratios (ORs) for posture exposures ranged from 1.1 to 2.0. Force exposure ORs ranged from 1.4 to 2.1. The magnitudes of the ORs differed according to the definition of low back pain, and heterogeneity was associated with both study-level and individual-level characteristics.

Conclusions. We found small to moderate ORs for the association of mechanical exposures and low back pain, although the relationships were complex. The presence of individual-level OR modifiers in such an area can be best understood by conducting a meta-analysis of individual participant data.
A Smoke-Free Paso del Norte: Impact Over 10 Years on Smoking Prevalence Using the Behavioral Risk Factor Surveillance System
Authors: Taylor Thom , Cooper Theodore V. , Hernandez Nora , Kelly Michael , Law Jon , Colwell Brian
Published: December 2011

Objectives. We assessed the impact of a tobacco control initiative over 10 years on cessation and prevention.

Methods. We examined 2000–2009 Behavioral Risk Factor Surveillance System cases of a metropolitan statistical area (MSA) with systematic tobacco control efforts throughout the decade (El Paso, TX) and 2 comparison MSAs similar in size and population with less coordinated tobacco control efforts (Austin-Round Rock, TX and San Antonio, TX).

Results. Yearly, El Paso exhibited a 6% increase in the prevalence of former smokers, a 6% decrease in prevalence of daily smokers, and a 7% decrease in the prevalence of established smoking (≥ 100 cigarettes per lifetime and currently smoking); we did not observe similar trends in the comparison MSAs. There was no change in the prevalence of nondaily smokers in any of the MSAs.

Conclusions. The coordinated tobacco control activities in El Paso are related to cessation among daily smokers and prevention of established smoking at the population level but have not stimulated cessation among nondaily smokers. Comprehensive tobacco control should focus more on not only daily smokers but also nondaily smokers.
Reduction in Purchases of Sugar-Sweetened Beverages Among Low-Income Black Adolescents After Exposure to Caloric Information
Authors: Bleich Sara N. , Herring Bradley J. , Flagg Desmond D. , Gary-Webb Tiffany L.
Published: December 2011

Objectives. We examined the effect of an intervention to provide caloric information about sugar-sweetened beverages (SSBs) on the number of SSB purchases.

Methods. We used a case-crossover design with 4 corner stores located in low-income, predominately Black neighborhoods in Baltimore, Maryland. The intervention randomly posted 1 of 3 signs with the following caloric information: (1) absolute caloric count, (2) percentage of total recommended daily intake, and (3) physical activity equivalent. We collected data for 1600 beverage sales by Black adolescents, aged 12–18 years, including 400 during a baseline period and 400 for each of the 3 caloric condition interventions.

Results. Providing Black adolescents with any caloric information significantly reduced the odds of SSB purchases relative to the baseline (odds ratio [OR] = 0.56; 95% confidence interval [CI] = 0.36, 0.89). When examining the 3 caloric conditions separately, the significant effect was observed when caloric information was provided as a physical activity equivalent (OR = 0.51; 95% CI = 0.31, 0.85).

Conclusions. Providing easily understandable caloric information—particularly a physical activity equivalent—may reduce calorie intake from SSBs among low-income, Black adolescents.
Social Norms, Collective Efficacy, and Smoking Cessation in Urban Neighborhoods
Authors: Karasek Deborah , Ahern Jennifer , Galea Sandro
Published: December 2011

Objectives. We examined the separate and combined relations of neighborhood-level social norms and collective efficacy with individuals’ cigarette smoking cessation.

Methods. We modeled the hazard of quitting over a 5-year period among 863 smokers who participated in the 2005 New York Social Environment Study.

Results. In adjusted Cox proportional hazard models, prohibitive neighborhood smoking norms were significantly associated with higher rates of smoking cessation (second quartile hazard ratio [HR] = 1.17; 95% confidence interval [CI] = 0.59, 2.32; third quartile HR = 2.37; 95% CI = 1.17, 4.78; fourth quartile HR = 1.80; 95% CI = 0.85, 3.81). We did not find a significant association between neighborhood collective efficacy and cessation or significant evidence of a joint relation of collective efficacy and smoking norms with cessation.

Conclusions. Neighborhood social norms may be more relevant than is collective efficacy to smoking cessation. The normative environment may shape health behavior and should be considered as part of public health intervention efforts.
Longitudinal Associations Between Adolescent Alcohol Use and Adulthood Sexual Risk Behavior and Sexually Transmitted Infection in the United States: Assessment of Differences by Race
Authors: Khan Maria R. , Berger Amanda T. , Wells Brooke E. , Cleland Charles M.
Published: December 2011

Objectives. We examined race differences in the longitudinal associations between adolescent alcohol use and adulthood sexually transmitted infection (STI) risk in the United States.

Methods. We estimated multivariable logistic regression models using Waves I (1994–1995: adolescence) and III (2001–2002: young adulthood) of the National Longitudinal Study of Adolescent Health (n = 10 783) to estimate associations and assess differences between Whites and African Americans.

Results. In adjusted analyses, adolescent alcohol indicators predicted adulthood inconsistent condom use for both races but were significantly stronger, more consistent predictors of elevated partnership levels for African Americans than Whites. Among African Americans but not Whites, self-reported STI was predicted by adolescent report of any prior use (adjusted odds ratio [AOR] = 1.47; 95% confidence interval [CI] = 1.00, 2.17) and past-year history of getting drunk (AOR = 1.53; 95% CI = 1.01, 2.32). Among Whites but not African Americans, biologically confirmed STI was predicted by adolescent report of past-year history of getting drunk (AOR = 1.68; 95% CI = 1.07, 2.63) and consistent drinking (AOR = 1.65; 95% CI = 1.03, 2.65).

Conclusions. African American and White adolescent drinkers are priority populations for STI prevention. Prevention of adolescent alcohol use may contribute to reductions in adulthood STI risk.
Effect of Same-Sex Marriage Laws on Health Care Use and Expenditures in Sexual Minority Men: A Quasi-Natural Experiment
Authors: Hatzenbuehler Mark L. , O'Cleirigh Conall , Grasso Chris , Mayer Kenneth , Safren Steven , Bradford Judith
Published: December 2011

Objectives. We sought to determine whether health care use and expenditures among gay and bisexual men were reduced following the enactment of same-sex marriage laws in Massachusetts in 2003.

Methods. We used quasi-experimental, prospective data from 1211 sexual minority male patients in a community-based health center in Massachusetts.

Results. In the 12 months after the legalization of same-sex marriage, sexual minority men had a statistically significant decrease in medical care visits (mean = 5.00 vs mean = 4.67; P = .05; Cohen's d = 0.17), mental health care visits (mean = 24.72 vs mean = 22.20; P = .03; Cohen's d = 0.35), and mental health care costs (mean = $2442.28 vs mean = $2137.38; P = .01; Cohen's d = 0.41), compared with the 12 months before the law change. These effects were not modified by partnership status, indicating that the health effect of same-sex marriage laws was similar for partnered and nonpartnered men.

Conclusions. Policies that confer protections to same-sex couples may be effective in reducing health care use and costs among sexual minority men.
Identifying Unaddressed Systemic Health Conditions at Dental Visits: Patients Who Visited Dental Practices but Not General Health Care Providers in 2008
Authors: Strauss Shiela M. , Alfano Michael C. , Shelley Donna , Fulmer Terry
Published: December 2011

We assessed the proportion and characteristics of patients who do not regularly visit general health care providers but do visit dentists and whose unaddressed systemic health conditions could therefore be identified by their dentist. Of the 26.0% of children and 24.1% of adults that did not access general outpatient health care in 2008, 34.7% and 23.1%, respectively, visited a dentist. They varied by census region, family income, and sociodemographics. Dental practices can serve as alternate sites of opportunity to identify health concerns among diverse groups of US patients.
The Late Cenozoic evolution of the Aksu basin (Isparta Angle; SW Turkey). New insights
Authors: Poisson André , Orszag-Sperber Fabienne , Kosun Erdal , Bassetti Maria-Angella , Müller Carla , Wernli Roland , Rouchy Jean-Marie
Published: December 2011

The Mio-Pliocene basins around the Antalya gulf in SW Turkey developed above the Tauric Mesozoic platforms on which the Antalya nappes had been thrusted (in Late Cretaceous-Paleocene times). The closure of the initial Isparta Angle during these events (E-W compression) initiated the N-S orientation of the main structural lines, which persisted later and explains the orientation of the Aksu basin in contrast with the E-W orientation of the eastern Neo-gene Mediterranean basins. The area, and all southwestern Turkey, became emergent at the end of the Oligocene and were the site of shallow-marine carbonate deposits in the Chattian-Aquitanian, giving way to the wide Lycian basin in Burdigalian-Langhian times. The progressive emplacement of the Lycian nappes from the north over this basin provoked first its subsidence and then its emersion when the nappes attained their final position over the Bey Daglari platform in Langhian times. Coinciding, or in response to the Lycian nappes emplacement, the Aksu basin was initiated as an elongated N-S graben which was filled by thick accumulations of terrestrial and marine deposits(including coral reefs), which derived from the erosion of the Lycian allochton and its basement (Langhian?, Serravallian and Tortonian times). The syn-sedimentary tectonics : reactivation of the normal faults along the west margin of the basin, the continuous uplift of the neighbouring continental areas (beginning of the Aksu thrust), governed the geometry of the basin. As a result and due to the uplift of its northern margin, the Aksu basin migrated towards the south and in Messinian times it was reduced to a narrow gulf along the eastern margin of which the Gebiz limestones were deposited as fringing coral reefs. The age of these limestones has been debated. Our new data allow us to attribute them to the Messinian. The drastic retreat of the sea at the end of this period, provoked the erosion of large parts of the Messinian deposits and the formation of deep canyons on land and under the sea down to the Antalya abyssal plain, in which evaporites were deposited. During the Zanclean transgression, the Eskiköy-Kargi canyon was filled by coarse clastics of a Gilbert delta derived from the northern continental area following a model well known elsewhere in the Mediterranean basins. Southward, shallow-marine sands and marls unconformably cover the remnants of the Messinian deposits and the emergent areas of the southern Antalya gulf. After Zanclean times (end of Pliocene?), the Aksu basin was deformed, due to the west-directed Aksu compressional event (end of the Aksu thrust). Quaternary terraces of the Aksu river at various altitudes, as well as the terraces of the Antalya tufa can be related to sea level fluctuations.