bluuebunny/crossref_metadata_2025 huggingface dataset:
Revenue Diversification, Risk and Bank Performance of Vietnamese Commercial Banks
Authors: Ngoc Nguyen Khanh
Published: August 2019

In the future, when the process of economic integration in the banking sector is more powerful, and competitive, diversifying revenue is an inevitable and objective trend to help the banks increase profits, minimize risks and improve their competitive position in the system. The research is on the relationship between revenue diversification, risk and bank performance using data from audited financial statements and annual reports of 26 commercial banks listed and unlisted in Vietnam during the period 2010–2018. The research method uses Generalized Method of Moment (GMM) modeling techniques to solve endogenous problems, variance and autocorrelation in the research model. Research results show that diversification negatively impacts profitability and the higher the diversification, the higher the risk of commercial banks. However, the more diversified listed banks, the more increased the bank’s stability. The banks show the weakness and lack of experience of the banking system in developing a reasonable profit transformation model. The revenue diversification of banks is currently passive and moves slowly. Interest income is still the motivation of bank development, boosting profit growth. Growth, as well as the contribution from service activities, is not commensurate with potentials; although there are many positive points, they are not enough to cover risks from net interest income activities.
Farms or Forests? Understanding and Mapping Shifting Cultivation Using the Case Study of West Garo Hills, India
Authors: Kurien Amit John , Lele Sharachchandra , Nagendra Harini
Published: August 2019

Attempts to study shifting cultivation landscapes are fundamentally impeded by the difficulty in mapping and distinguishing shifting cultivation, settled farms and forests. There are foundational challenges in defining shifting cultivation and its constituent land-covers and land-uses, conceptualizing a suitable mapping framework, and identifying consequent methodological specifications. Our objective is to present a rigorous methodological framework and mapping protocol, couple it with extensive fieldwork and use them to undertake a two-season Landsat image analysis to map the forest-agriculture frontier of West Garo Hills district, Meghalaya, in Northeast India. We achieve an overall accuracy of ~80% and find that shifting cultivation is the most extensive land-use, followed by tree plantations and old-growth forest confined to only a few locations. We have also found that commercial plantation extent is positively correlated with shortened fallow periods and high land-use intensities. Our findings are in sharp contrast to various official reports and studies, including from the Forest Survey of India, the Wastelands Atlas of India and state government statistics that show the landscape as primarily forested with only small fractions under shifting cultivation, a consequence of the lack of clear definitions and poor understanding of what constitutes shifting cultivation and forest. Our results call for an attentive revision of India’s official land-use mapping protocols, and have wider significance for remote sensing-based mapping in other shifting cultivation landscapes.
3D Viscoplastic Finite Element Modeling of Dislocation Generation in a Large Size Si Ingot of the Directional Solidification Stage
Authors: Lin Maohua , Wu Xinjiang , Liao Xinqin , Shi Min , Ou Disheng , Tsai Chi-Tay
Published: August 2019

Growing very large size silicon ingots with low dislocation density is a critical issue for the photovoltaic industry to reduce the production cost of the high-efficiency solar cell for affordable green energy. The thermal stresses, which are produced as the result of the non-uniform temperature field, would generate dislocation in the ingot. This is a complicated thermal viscoplasticity process during the cooling process of crystal growth. A nonlinear three-dimensional transient formulation derived from the Hassen-Sumino model (HAS) was applied to predict the number of dislocation densities, which couples the macroscopic viscoplastic deformation with the microscopic dislocation dynamics. A typical cooling process during the growth of very large size (G5 size: 0.84 m × 0.84 m × 0.3 m) Si ingot is used as an example to validate the developed HAS model and the results are compared with those obtained from qualitatively critical resolved shear stress model (CRSS). The result demonstrates that this finite element model not only predicts a similar pattern of dislocation generation with the CRSS model but also anticipate the dislocation density quantity generated in the Si ingot. A modified cooling process is also employed to study the effect of the cooling process on the generation of the dislocation. It clearly shows that dislocation density is drastically decreased by modifying the cooling process. The results obtained from this model can provide valuable information for engineers to design a better cooling process for reducing the dislocation density produced in the Si ingot under the crystal growth process.
On-Site Health Monitoring of Composite Bolted Joint Using Built-In Distributed Eddy Current Sensor Network
Authors: Liu Qijian , Sun Hu , Wang Tao , Qing Xinlin
Published: August 2019

There is an urgent need to monitor the structural state of composite bolted joints while still remaining in service; however, there are many difficulties in analyzing their strength and failure modes. In this paper, a built-in distributed eddy current (EC) sensor network based on EC array sensing film is developed to monitor the hole-edge damages of composite bolted joints. The EC array sensing film is bonded onto the bolt and consists of one exciting coil and four separate sensing coils. Experiments are conducted on unidirectional composite specimens to validate the ability of the EC array sensing film to quantitatively track the damage that occurs at the hole edge and to investigate the performances of the EC array sensing films with different configurations of the exciting coil. Experimental results show that the induced voltage of sensing coil changes only if the damage appears on the laminate structure where that particular sensing coil is located, whereas the induced voltages of the other sensing coils on other laminate plates remain unchanged. Numerical simulation based on the finite element method is also carried out to investigate and explain the phenomena observed in the experiments and to analyze the distribution of the EC around the bolt hole. Both experimental and numerical simulation results demonstrate that the developed EC array sensing film can effectively identify not only whether there is damage at the hole edge but also the damage location within the thickness and quantitative size.
Characterization, Recombinant Production and Structure-Function Analysis of NvCI, A Picomolar Metallocarboxypeptidase Inhibitor from the Marine Snail Nerita versicolor
Authors: Covaleda-Cortés Giovanni , Hernández Martha , Trejo Sebastián Alejandro , Mansur Manuel , Rodríguez-Calado Sergi , García-Pardo Javier , Lorenzo Julia , Vendrell Josep , Chávez María Ángeles , Alonso-del-Rivero Maday , Avilés Francesc Xavier
Published: August 2019

A very powerful proteinaceous inhibitor of metallocarboxypeptidases has been isolated from the marine snail Nerita versicolor and characterized in depth. The most abundant of four, very similar isoforms, NvCla, was taken as reference and N-terminally sequenced to obtain a 372-nucleotide band coding for the protein cDNA. The mature protein contains 53 residues and three disulphide bonds. NvCIa and the other isoforms show an exceptionally high inhibitory capacity of around 1.8 pM for human Carboxypeptidase A1 (hCPA1) and for other A-like members of the M14 CPA subfamily, whereas a twofold decrease in inhibitory potency is observed for carboxypeptidase B-like members as hCPB and hTAFIa. A recombinant form, rNvCI, was produced in high yield and HPLC, mass spectrometry and spectroscopic analyses by CD and NMR indicated its homogeneous, compact and thermally resistant nature. Using antibodies raised with rNvCI and histochemical analyses, a preferential distribution of the inhibitor in the surface regions of the animal body was observed, particularly nearby the open entrance of the shell and gut, suggesting its involvement in biological defense mechanisms. The properties of this strong, small and stable inhibitor of metallocarboxypeptidases envisage potentialities for its direct applicability, as well as leading or minimized forms, in biotechnological/biomedical uses.
Erectile Dysfunction: A Primer for in Office Management
Authors: Sami Samir , Stern Noah , Di Pierdomenico Andrew , Katz Brandon , Brock Gerald
Published: August 2019

Introduction: Optimizing erectile dysfunction (ED) remains a clinically significant endeavor as insufficient outcomes from oral, injectable and even surgical approaches to treatment remain less than ideal. In this report, we integrate evolving knowledge and provide an algorithmic approach for the clinician to fine-tune management. Methods: We performed a PubMed and Medline search of Erectile Dysfunction treatment optimization, enhanced patient efficacy for ED, and why men fail ED treatment. All relevant papers for the past two decades were reviewed. Results: Establishing the goals and objectives of the patient and partner while providing detailed instructions for treatment can minimize failures and create an environment that allows treatment optimization. A thorough work-up may identify reversible or contributing causes. We identified several areas where treatment of ED could be optimized. These include; management of associated medical conditions, lifestyle improvements, PDE5 inhibitor prescription strategies, management of hypogonadism and the initiation of intracavernosal injection therapy (ICI). Conclusions: In our view, once a man presents for help to the clinician, use of the simple strategies identified in this review to optimize the tolerability, safety and effectiveness of the selected treatment should result in enhanced patient and partner satisfaction, with improved outcomes.
A Review of Current and Emerging Therapeutic Options for Erectile Dysfunction
Authors: Chung Eric
Published: August 2019

Contemporary treatment algorithms for erectile dysfunction (ED) involve the use of medical therapies such as phosphodiesterase type 5 (PDE5) inhibitors and intracavernosal injection therapy of vasoactive agents, as well as vacuum erection devices and penile prosthesis implants in medically refractory cases. However, the current therapeutic options only address the symptoms of ED and not the underlying pathogenesis that results in ED. Newer and novel ED therapies aspire to reverse ED conditions by preventing cavernosal fibrosis, promoting endothelial revascularization and modulating various neuro-hormonal pathways. Regenerative therapeutic strategies such as low-intensity shock wave, gene and cellular-based therapies, and penile transplants are designed to improve penile hemodynamics and revitalize the cavernosal smooth muscle to mitigate and/or reverse underlying ED. This state-of-art article evaluates current and emerging therapeutic options for ED.
Friction Drilling of Difficult-to-Machine Materials: Workpiece Microstructural Alterations and Tool Wear
Authors: Dehghan Shayan , Ismail Mohd Idris Shah b. , Ariffin Mohd Khairol Anuar b. Mohd , Baharudin B. T. Hang Tuah b.
Published: August 2019

Difficult-to-machine materials are metals that have great toughness, high work-hardening, and low thermal conductivity. Friction drilling of difficult-to-machine materials is a technically challenging task due to the difficulty of friction drilling, leading to excessive tool wear, which adversely affects surface integrity and product performance. In the present study, the microstructural changes of workpieces and tool wear for friction drilling of AISI304, Ti-6Al-4V, and Inconel718 are characterized. It helps to have an in-depth understanding of heat generation mechanics by friction and the mechanism of the friction drilling process. The study contributes to providing an enhanced microstructural characterization of workpiece and tool conditions, which identifies the material behavior and shows how it affects the bushing formation quality and drilling tool performance. The results reveal that the abrasive wear is mostly observed in the conical region of the tool, which has maximum contact with hole-wall. Moreover, the low thermal conductivity of Ti-6Al-4V increases frictional heat generation severely, and reduces product quality and tool life subsequently.
Investigation of Geometric Characteristics in Curved Surface Laser Cladding with Curve Path
Authors: Lian Guofu , Zhang Hao , Zhang Yang , Huang Xu , Chen Changrong , Jiang Jibin
Published: August 2019

Laser cladding on curved surfaces is essential in industrial applications for restoration and remanufacturing of high-value parts. This study investigated the influence of different factors on clad width, clad height, and dilution rate in curved surface laser cladding with curved path. Mathematical models were developed using central composite designs to predict these geometric characteristics by controlling laser power, scanning speed, gas flow, and altering the outside radius of the cylindrical substrate. Analysis of variance and response surface methodology indicated that clad width increased with increasing laser power and reducing scanning speed. Clad height positively correlated to laser power and negatively correlated to the outside radius of the cylindrical substrate. Increasing the laser power while decreasing the scanning speed led to an increase in dilution rate. Afterwards, the geometric characteristics of the clad were improved by optimizing these factors with the target to maximize clad width and height as well as to minimize dilution rate. The difference between model predictions and experimental validations for clad width, clad height, and dilution rate were 3.485%, 3.863%, and 6.566%, respectively. The predicted accuracy was verified with these models, and they were able to provide theoretical guidance to predict and control the geometric characteristics of curved surface laser cladding with a curved path.
A Polyphasic Approach Aids Early Detection of Potentially Toxigenic Aspergilli in Soil
Authors: Cafà Giovanni , Caggiano Benedetta , Reeve Michael A. , Bhatti Hamzah , Honey Sabyan F. , Bajwa Babar , Buddie Alan G.
Published: August 2019

Key chili and maize growing areas of Pakistan were selected for a focused baseline study of the levels of Aspergillus spp. Investigations were undertaken using a combination of molecular and culture-based techniques. Samples investigated included soil samples, one-year-old corn cobs, and fresh chili from selected locations. Aspergillus strains obtained from corn cobs were screened using coconut milk agar, resulting in one strain that was positive for aflatoxin production. Whole genome sequencing (WGS) with low coverage techniques were employed to screen the isolates for differences in the ribosomal RNA gene cluster and mitochondrial genome, with the aflatoxigenic strain proving to have a distinctive profile. Finally, strains were subjected to matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry (MALDI-ToF-MS) in order to obtain a proteomic ‘fingerprint’ which was used to distinguish the aflatoxigenic strain from the other isolates. The next generation sequencing (NGS) study was broadened to incorporate metabarcoding with ITS rRNA for determining the microbial biodiversity of the soil samples and presumptive screening for the presence of aflatoxigenic strains. Using information gleaned from the WGS results, a putative aflatoxigenic operational taxonomic unit (OTU) was observed in four of the 15 soil samples screened by metabarcoding. This method may have beneficial applications in early detection and surveillance programs in agricultural soils and commodities.
Thermal Transport Properties of Olivine, Wadsleyite, and Ringwoodite—A Review
Authors: Xiong Zili , Zhang Baohua
Published: August 2019

Knowledge of the thermal diffusivity D and thermal conductivity κ of olivine and its high-pressure polymorphs, wadsleyite and ringwoodite, is crucial to understand the heat transfer efficiency and thermal structure of Earth’s mantle. In the last few decades, great progress has been made in measurement techniques and experimental results seeking to determine the thermophysical properties of geomaterials. The aim of this paper is to provide a systematic overview of the current knowledge of the thermal transport properties of olivine and its high-pressure polymorphs, their dependence on factors, such as pressure, temperature, and composition, and physical models to describe their variations. Some related geoscience applications and perspectives for the future are also suggested.
Uncertainty and Sensitivity Analysis at Low Value of Determination Coefficient of Regression Analysis: Case of I-129 Release from RBMK-1500 SNF under Disposal Conditions
Authors: Narkuniene Asta , Poskas Povilas , Justinavicius Darius
Published: August 2019

As in other nuclear countries, the operation of the Ignalina nuclear power plant in Lithuania has led to the accumulation of around 22 thousand assemblies of spent nuclear fuel (SNF). The development of geological disposal program involves an iterative assessment of the system safety supported by scientific research on radionuclides migration and related processes. This study focused on the application of Contribution to the Sample Mean (CSM) and Contribution to Sample Variance (CSV) methods to complement the uncertainty and sensitivity analyses of the time-dependent flux of I-129 from the engineered barriers of a conceptual disposal facility for RBMK-1500 SNF (RBMK is abbreviation of “High Power Channel-type Reactor” (in Russian)). The analysis was performed using a MATLAB platform (8.0.0.783 (R2012b), MathWorks, MA, USA). The mean and variance ratios derived from CSM and CSV plots were applied to estimate the effect of reduced uncertainty range on mean flux and its variance, and the uncertainty analysis was also complimented. Increasing the lower bounding value of defect size enlargement time range to 4.6 × 104 years would lead to a lower mean flux until 5 × 104 years after repository closure. Later on (up to 1 million years after repository closure), the only reduction of the upper bounding value of the SNF dissolution rate range would affect a decreased mean flux.
Selective Leaching of Rare Earth Elements (REEs) from Eudialyte Concentrate after Sulfation and Thermal Decomposition of Non-REE Sulfates
Authors: Balinski Adam , Atanasova Petya , Wiche Oliver , Kelly Norman , Reuter Markus Andreas , Scharf Christiane
Published: August 2019

Eudialyte, a sodium rich zirconosilicate, is one of the promising sources for REEs (rare earth elements), particularly for HREEs + Y (heavy rare earth elements and yttrium). The key challenge in hydrometallurgical processing is the prevention of silica gel formation and REE separation from resulting multi-element leach solutions. This study deals with the selective extraction of REE from eudialyte concentrate by selective roasting. In this method, metal ions are converted into sulfates, followed by the decomposition of non-REE sulfates in a roasting step and the water leaching of the calcine. The effect of acid addition, roasting temperature, roasting time, pulp density and leaching time is studied. For sufficient conversion of REEs into sulfates, sulfuric acid is added in excess. At a roasting temperature of ≥750 °C sulfates of zirconium, hafnium, niobium, aluminum and iron decompose into sparingly soluble compounds, while REE and manganese sulfates remain stable up to a roasting time of 120 min. The silica present in the calcine is found to be metastable even after roasting. The amount of leached Si4+ is dependent predominantly on the pH value of the leaching medium. Applying the method, REEs can be efficiently separated from zirconium, hafnium, niobium, aluminum and iron. However, only diluted solutions can be produced. Water leaching of calcine at high solid/liquid ratios causes REE losses resulting from formation of double sulfates and gypsum. The acid excess removed from the reaction mixture in the roasting stage can be simply recovered by treatment of the gas phase.
Sensor-Based Ore Sorting Technology in Mining—Past, Present and Future
Authors: Robben Christopher , Wotruba Hermann
Published: August 2019

While the deposit qualities for mineral raw materials are constantly decreasing, the challenges for sustainable raw material processing are increasing. This applies not only to the demand for minimizing the consumption of energy, water, and reagents, but also to the reduction of residual materials, especially fine and difficult-to-landfill materials. Sensor-based ore sorting can be used as a separation process for coarser grain sizes before the application of fine comminution and separation technologies and is applicable for a large variety of mineral raw materials. Sensor-based ore sorting applies at various points in the process flow diagram and is suitable for waste elimination, for material diversion into different process lines, for the production of pre- and final concentrates, as well as for the reprocessing of coarse-grained waste dumps and other applications. The article gives an overview of the development and state of the art of sensor-based ore sorting for mineral raw materials and introduces various applications.
Etlingera elatior-Mediated Synthesis of Gold Nanoparticles and Their Application as Electrochemical Current Enhancer
Authors: Azri Farah Asilah , Selamat Jinap , Sukor Rashidah , Yusof Nor Azah , Ahmad Raston Nurul Hanun , Nordin Noordiana , Jambari Nuzul Noorahya
Published: August 2019

This work presents a simple green synthesis of gold nanoparticles (AuNPs) by using an aqueous extract of Etlingera elatior (torch ginger). The metabolites present in E. elatior, including sugars, proteins, polyphenols, and flavonoids, were known to play important roles in reducing metal ions and supporting the subsequent stability of nanoparticles. The present work aimed to investigate the ability of the E. elatior extract to synthesise AuNPs via the reduction of gold (III) chloride hydrate and characterise the properties of the nanoparticles produced. The antioxidant properties of the E. elatior extract were evaluated by analysing the total phenolic and total flavonoid contents. To ascertain the formation of AuNPs, the synthesised particles were characterised using the ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HRTEM), energy-dispersive X-ray (EDX) microscopy, and dynamic light scattering (DLS) measurement. The properties of the green synthesised AuNPs were shown to be comparable to the AuNPs produced using a conventional reducing agent, sodium citrate. The UV-Vis measured the surface plasmon resonance of the AuNPs, and a band centered at 529 nm was obtained. The FTIR results proved that the extract contained the O-H functional group that is responsible for capping the nanoparticles. The HRTEM images showed that the green synthesized AuNPs were of various shapes and the average of the nanoparticles’ hydrodynamic diameter was 31.5 ± 0.5 nm. Meanwhile, the zeta potential of −32.0 ± 0.4 mV indicates the high stability and negative charge of the AuNPs. We further successfully demonstrated that using the green synthesised AuNPs as the nanocomposite to modify the working surface of screen-printed carbon electrode (SPCE/Cs/AuNPs) enhanced the rate of electron transfer and provided a sensitive platform for the detection of Cu(II) ions.
Evaluation of the Antifungal Activity of Mentha x piperita (Lamiaceae) of Pancalieri (Turin, Italy) Essential Oil and Its Synergistic Interaction with Azoles
Authors: Tullio Vivian , Roana Janira , Scalas Daniela , Mandras Narcisa
Published: August 2019

The promising antimicrobial activity of essential oils (EOs) has led researchers to use them in combination with antimicrobial drugs in order to reduce drug toxicity, side effects, and resistance to single agents. Mentha x piperita, known worldwide as “Mentha of Pancalieri”, is produced locally at Pancalieri (Turin, Italy). The EO from this Mentha species is considered as one of the best mint EOs in the world. In our research, we assessed the antifungal activity of “Mentha of Pancalieri” EO, either alone or in combination with azole drugs (fluconazole, itraconazole, ketoconazole) against a wide panel of yeast and dermatophyte clinical isolates. The EO was analyzed by GC-MS, and its antifungal properties were evaluated by minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) parameters, in accordance with the CLSI guidelines, with some modifications. The interaction of EO with azoles was evaluated through the chequerboard and isobologram methods. The results suggest that this EO exerts a fungicidal activity against yeasts and a fungistatic activity against dermatophytes. Interaction studies with azoles indicated mainly synergistic profiles between itraconazole and EO vs. Candida spp., Cryptococcus neoformans, and Trichophyton mentagrophytes. Thus, the “Mentha of Pancalieri” EO may act as a potential antifungal agent and could serve as a natural adjuvant for fungal infection treatment.
Identification of New Antifungal Agents Targeting Chitin Synthesis by a Chemical-Genetic Method
Authors: Li Yan , Sun Hongmin , Zhu Xiaohong , Bian Cong , Wang Yanchang , Si Shuyi
Published: August 2019

Fungal infection is a leading cause of mortality in immunocompromised population; thus, it is urgent to develop new and safe antifungal agents. Different from human cells, fungi have a cell wall, which is composed mainly of polysaccharide glucan and chitin. The unique cell wall structure is an ideal target for antifungal drugs. In this research, a chemical-genetic method was used to isolate antifungal agents that target chitin synthesis in yeast cells. From a compound library, we isolated two benzothiazole compounds that showed greater toxicity to yeast mutants lacking glucan synthase Fks1 compared to wild-type yeast cells and mutants lacking chitin synthase Chs3. Both of them inhibited the activity of chitin synthase in vitro and reduced chitin level in yeast cells. Besides, these compounds showed clear synergistic antifungal effect with a glucan synthase inhibitors caspofungin. Furthermore, these compounds inhibited the growth of Saccharomyces cerevisiae and opportunistic pathogen Candida albicans. Surprisingly, the genome-wide mass-spectrometry analysis showed decreased protein level of chitin synthases in cells treated with one of these drugs, and this decrease was not a result of downregulation of gene transcription. Therefore, we successfully identified two new antifungal agents that inhibit chitin synthesis using a chemical-genetic method.
Enhanced Cycle Stability of Zinc Sulfide Anode for High-Performance Lithium-Ion Storage: Effect of Conductive Hybrid Matrix on Active ZnS
Authors: Nguyen Quoc Hanh , Park Taehyun , Hur Jaehyun
Published: August 2019

Zinc sulfide (ZnS) nanocrystallites embedded in a conductive hybrid matrix of titanium carbide and carbon, are successfully fabricated via a facile high-energy ball-milling (HEBM) process. The structural and morphological analyses of the ZnS-TiC-C nanocomposites reveal that ZnS and TiC nanocrystallites are homogeneously distributed in an amorphous carbon matrix. Compared with ZnS-C and ZnS composites, the ZnS-TiC-C nanocomposite exhibits significantly improved electrochemical performance, delivering a highly reversible specific capacity (613 mA h g−1 over 600 cycles at 0.1 A g−1, i.e., ~85% capacity retention), excellent long-term cyclic performance (545 mA h g−1 and 467 mA h g−1 at 0.5 A g−1 and 1 A g−1, respectively, after 600 cycles), and good rate capability at 10 A g−1 (69% capacity retention at 0.1 A g−1). The electrochemical performance is significantly improved, primarily owing to the presence of conductive hybrid matrix of titanium carbide and amorphous carbon in the ZnS-TiC-C nanocomposites. The matrix not only provides high conductivity but also acts as a mechanical buffering matrix preventing huge volume changes during prolonged cycling. The lithiation/delithiation mechanisms of the ZnS-TiC-C electrodes are examined via ex situ X-ray diffraction (XRD) analysis. Furthermore, to investigate the practical application of the ZnS-TiC-C nanocomposite, a coin-type full cell consisting of a ZnS-TiC-C anode and a LiFePO4–graphite cathode is assembled and characterized. The cell exhibits excellent cyclic stability up to 200 cycles and a good rate performance. This study clearly demonstrates that the ZnS-TiC-C nanocomposite can be a promising negative electrode material for the next-generation lithium-ion batteries.
Thermal Flow Self-Assembled Anisotropic Chemically Derived Graphene Aerogels and Their Thermal Conductivity Enhancement
Authors: Huang Jinhui , Zhang Buning , Valdiserri Paolo , Huang Xue , Yin Guoqiang , Cui Yingde
Published: August 2019

In this study, we investigated the directional heating of graphene oxide (GO) dispersion to generate a temperature gradient and form a simulated “ocean current” inside the dispersion so that GO sheets could be aligned in a directional manner and then reduced and self-assembled into anisotropic reduced graphene oxide (rGO) gel. After freeze-drying and varying degrees of vacuum microwave treatment, anisotropic chemically derived graphene aerogels (AGAs) were obtained. Through performance detection and the analysis of the results, it was verified that the AGAs with certain characteristics of “ocean current” were prepared in this experiment, and its axial direction has obvious directional arrangement. After being treated by vacuum microwave for a short time (1 min.), the axial thermal conductivity of the composite materials (AGA-adsorbed paraffin) was observed to be 1.074 W/mK, and the thermal conductivity enhancement efficiency was 995%; as compared with similar thermal conductivity enhancement composites that were found in previous studies, the proposed method in this paper has the advantages of simple processing, high efficiency, and energy conservation.
Elasticity of Cross-Linked Titania Nanocrystal Assemblies Probed by AFM-Bulge Tests
Authors: Hensel Andreas , Schröter Clemens J. , Schlicke Hendrik , Schulz Norbert , Riekeberg Svenja , Trieu Hoc Khiem , Stierle Andreas , Noei Heshmat , Weller Horst , Vossmeyer Tobias
Published: August 2019

In order to enable advanced technological applications of nanocrystal composites, e.g., as functional coatings and layers in flexible optics and electronics, it is necessary to understand and control their mechanical properties. The objective of this study was to show how the elasticity of such composites depends on the nanocrystals’ dimensionality. To this end, thin films of titania nanodots (TNDs; diameter: ~3–7 nm), nanorods (TNRs; diameter: ~3.4 nm; length: ~29 nm), and nanoplates (TNPs; thickness: ~6 nm; edge length: ~34 nm) were assembled via layer-by-layer spin-coating. 1,12-dodecanedioic acid (12DAC) was added to cross-link the nanocrystals and to enable regular film deposition. The optical attenuation coefficients of the films were determined by ultraviolet/visible (UV/vis) absorbance measurements, revealing much lower values than those known for titania films prepared via chemical vapor deposition (CVD). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed a homogeneous coverage of the substrates on the µm-scale but a highly disordered arrangement of nanocrystals on the nm-scale. X-ray photoelectron spectroscopy (XPS) analyses confirmed the presence of the 12DAC cross-linker after film fabrication. After transferring the films onto silicon substrates featuring circular apertures (diameter: 32–111 µm), freestanding membranes (thickness: 20–42 nm) were obtained and subjected to atomic force microscopy bulge tests (AFM-bulge tests). These measurements revealed increasing elastic moduli with increasing dimensionality of the nanocrystals, i.e., 2.57 ± 0.18 GPa for the TND films, 5.22 ± 0.39 GPa for the TNR films, and 7.21 ± 1.04 GPa for the TNP films.
Mice Lacking the Intestinal and Renal Neutral Amino Acid Transporter SLC6A19 Demonstrate the Relationship between Dietary Protein Intake and Amino Acid Malabsorption
Authors: Javed Kiran , Bröer Stefan
Published: August 2019

Dietary protein restriction has beneficial impacts on metabolic health. B0AT1 (SLC6A19) is the major transporter of neutral amino acids at the intestinal epithelia and absorbs the bulk of the diet-derived neutral amino acids from the intestinal lumen. It also reabsorbs neutral amino acids in the renal proximal tubules. Mice lacking B0AT1 show cellular outcomes of protein restriction, such as high FGF21 levels and low mTORC1 activity. Moreover, they have improved glucose homeostasis and resist diet-induced obesity. In this study, we investigated the relationship between protein restriction and dietary protein intake in C57Bl6/J wild-type (wt) and SLC6A19-knockout (SLC6A19ko) mice. When SLC6A19ko mice were fed diets containing 5%, 25%, or 52% of their total calories derived from protein, no differences in food intake or weight gain were observed. All essential amino acids significantly positively correlated with increasing dietary casein content in the wt mice. The SLC6A19ko mice showed reduced postprandial levels of essential amino acids in plasma, particularly following high-protein diets. Upon fasting, essential amino acids were the same in the wt and SLC6A19ko mice due to reduced amino acid catabolism. Bacterial metabolites originating from amino acid fermentation correlated with the dietary protein content, but showed a complex profile in the blood of the SLC6A19ko mice. This study highlights the potential of SLC6A19 as a knock-out or inhibition target to induce protein restriction for the treatment of metabolic disorders.
Korean Red Ginseng Extract Increases Apoptosis by Activation of the Noxa Pathway in Colorectal Cancer
Authors: Jeong Yoon A. , Kim Bo Ram , Kim Dae Young , Jeong Soyeon , Na Yoo Jin , Kim Jung Lim , Yun Hye Kyeong , Kim Bu Gyeom , Park Seong Hye , Jo Min Jee , Lee Sun Il , Han Byung-Cheol , Lee Dae-Hee , Oh Sang Cheul
Published: August 2019

Background: Although the anticancer activity of Korean Red Ginseng (KRG) has been known in various cancers, the mechanism of KRG-induced apoptosis is unknown in colorectal cancer (CRC). In our study, we examined whether KRG induces apoptosis in CRC cells. Methods: In the cell viability assay, the concentration of the appropriate KRG extracts was fixed at 2.5 mg/mL in numerous CRC cells. This fixed concentration was in other experiments, and it was confirmed that the KRG extracts induce apoptosis in CRC cells. Results: We found that KRG induced Noxa activation and apoptosis and increased endoplasmic reticulum stress via reactive oxygen species production. This indicated that KRG efficiently enhanced cell death in CRC cells. Conclusion: Our results show that KRG can be used as a possible anticancer drug for patients with CRC
Effectiveness of Non-Animal Chondroitin Sulfate Supplementation in the Treatment of Moderate Knee Osteoarthritis in a Group of Overweight Subjects: A Randomized, Double-Blind, Placebo-Controlled Pilot Study
Authors: Rondanelli Mariangela , Braschi Valentina , Gasparri Clara , Nichetti Mara , Faliva Milena Anna , Peroni Gabriella , Naso Maurizio , Iannello Giancarlo , Spadaccini Daniele , Miraglia Niccolò , Putignano Pietro , Alalwan Tariq A. , Perna Simone
Published: August 2019

Osteoarthritis (OA) is the most common form of arthritis in the world and is characterized by pain, various disabilities and loss of quality of life. Chondroitin sulfate (CS) is recommended as first-line therapy. CS of non-animal origin is of great interest for safety and sustainability reasons. This study aims to investigate the anti-inflammatory effects, anti-pain and ability-enhancement of a short-term supplementation with non-animal CS in overweight subjects with OA. In a randomized, double-blind, placebo-controlled pilot study, 60 overweight adults with symptomatic OA were allocated to consume 600 mg of non-animal CS (n = 30) or a placebo (n = 30) daily for 12 consecutive weeks. The assessment of knee-pain, quality of life, related inflammation markers and body composition was performed at 0, 4 and 12 weeks. The Tegner Lysholm Knee Scoring (TLKS) scale of the experimental group showed a statistically significant increase (+10.64 points; confidence interval (95% confidence interval (CI) 5.57; 15.70; p < 0.01), while the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score decreased (−12.24 points; CI 95% −16.01; −8.38; p < 0.01). The results also showed a decrease in the C-reactive protein (CRP) level (−0.14 mg/dL, CI 95% −0.26; −0.04; p < 0.01) and erythrocyte sedimentation rate (ESR) level (−5.01 mm/h, CI 95% −9.18; −0.84, p < 0.01) as well as the visual analogue scale (VAS) score in both knees. In conclusion, this pilot study demonstrates the effectiveness of non-animal CS supplementation in overweight subjects with knee OA in improving knee function, pain and inflammation markers.
Sustainable Elimination (Zero Cases) of Sleeping Sickness: How Far Are We from Achieving This Goal?
Authors: Akazue Pearl Ihuoma , Ebiloma Godwin U. , Ajibola Olumide , Isaac Clement , Onyekwelu Kenechukwu , Ezeh Charles O. , Eze Anthonius Anayochukwu
Published: August 2019

The recent massive reduction in the numbers of fresh Human African Trypanosomiasis (HAT) infection has presented an opportunity for the global elimination of this disease. To prevent a possible resurgence, as was the case after the reduced transmission of the 1960s, surveillance needs to be sustained and the necessary tools for detection and treatment of cases need to be made available at the points of care. In this review, we examine the available resources and make recommendations for improvement to ensure the sustenance of the already achieved gains to keep the trend moving towards elimination.
Aminoglycoside Allergic Reactions
Authors: Childs-Kean Lindsey M. , Shaeer Kristy M. , Varghese Gupta Sheeba , Cho Jonathan C.
Published: August 2019

Aminoglycosides are antimicrobial agents that are primarily used for infections caused by Gram-negative pathogens. The purpose of this article is to review the allergic reactions reported in the published literature to aminoglycoside antibiotics. A thorough PubMed search was conducted and excluded non-allergic adverse reactions to aminoglycosides. Allergic reactions to aminoglycosides occur infrequently, but can include cutaneous reactions as well as systemic reactions, including anaphylaxis. Of the evaluated aminoglycosides, gentamicin had the most reported allergic reactions, including the most reports of anaphylaxis, followed by tobramycin, and then amikacin. Most reports of allergic reactions occurred in patients who had a prior exposure to some dosage form of an aminoglycoside. Cross-reactivity among aminoglycosides is common and occurs due to the similarities in their chemical structures. Desensitization protocols to tobramycin have been described in the literature.
Ultrafast Hyperspectral Transient Absorption Spectroscopy: Application to Single Layer Graphene
Authors: Gesuele Felice
Published: August 2019

We describe the basic principles and the experimental implementation of the hyperspectral transient absorption technique, based on femtosecond laser sources. In this technique the samples were optically “pumped” using the femtosecond tunable pulse delivered by an Optical Parametric Amplifier, and “probed” for changes in transmission in a broad spectral range with a “white light” laser-generated supercontinuum. The spectra were collected by a pair of multichannel detectors which allowed retrieval of the absorbance change in a wide spectral range in one time. The use of the supercontinuum probe introduced artifacts in the measured 2D data set which could be corrected with a proper calibration of the chirp. The configuration with crossed polarization for pump and probe pulse extended the spectral measured range above and below the pump energy within the same experiment. We showed the versatility of the technique by applying it to the investigation of the charge carrier dynamics in two-dimensional single layer graphene.
Effect of Long-Term Fungicide Applications on Virulence and Diversity of Colletotrichum spp. Associated to Olive Anthracnose
Authors: Materatski Patrick , Varanda Carla , Carvalho Teresa , Dias António Bento , Campos Maria Doroteia , Gomes Luis , Nobre Tânia , Rei Fernando , Félix Maria do Rosário
Published: August 2019

In this study, the presence and variability of Colletotrichum spp. was evaluated by comparing fungal isolates obtained from olive trees under long-time phytosanitary treatments with trees without any phytosanitary treatments (treated and untreated, respectively). Olive fruits of trees of the highly susceptible ‘Galega vulgar’ cultivar growing in the Alentejo region were used as samples. From the 210 olive trees sampled (half from treated and half from untreated orchards), 125 (59.5%) presented Colletotrichum spp., with a significant lower number of infected trees in treated (39) when compared to untreated orchards (86). The alignment and analysis of beta-tubulin (tub2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), chitin synthase (CHS-1) and histone H3 (HIS-3) gene sequences allowed the identification of all 125 isolates as belonging to the C. acutatum complex. The vast majority of the isolates (124) were identified as C. nymphaeae and one isolate, from an untreated tree, was identified as C. godetiae. Isolates were divided into five different groups: Group A: 39 isolates from treated trees matched in 100% with C. nymphaeae sequences from the database; Group B: 76 isolates from untreated trees matched in 100% with C. nymphaeae sequences from the database; Group C: one isolate from untreated trees presenting a single nucleotidic difference in the HIS-3 sequence; Group D: eight isolates from untreated trees presenting differences in two nucleotides in the tub2 sequences that changed the protein structure, together with differences in two specific nucleotides of the GAPDH sequences; Group E: one isolate, from untreated olive trees, matched 100% with C. godetiae sequences from the database in all genes. Considering the similarities of the sampled areas, our results show that the long-time application of fungicides may have caused a reduction in the number of olive trees infected with Colletotrichum spp. but an increase in the number of fruits positive to Colletotrichum spp. within each tree, which may suggest different degrees of virulence of Colletotrichum isolates from trees growing different management regimes. It is imperative that the fungicides described as causing resistance are applied at appropriate times and intervals, since their efficiency decreases when applied incorrectly and new and more virulent species may arise.
Valuation of Soil Organic Carbon Stocks in the Contiguous United States Based on the Avoided Social Cost of Carbon Emissions
Authors: Mikhailova Elena A. , Groshans Garth R. , Post Christopher J. , Schlautman Mark A. , Post Gregory C.
Published: August 2019

Soil organic carbon (SOC) generates several ecosystem services (ES), including a regulating service by sequestering carbon (C) as SOC. This ES can be valued based on the avoided social cost of carbon (SC-CO2) from the long-term damage resulting from emissions of carbon dioxide (CO2). The objective of this study was to assess the value of SOC stocks, based on the avoided SC-CO2 ($42 per metric ton of CO2 in 2007 U.S. dollars), in the contiguous United States (U.S.) by soil order, soil depth (0–20, 20–100, 100–200 cm), land resource region (LRR), state, and region using information from the State Soil Geographic (STATSGO) database. The total calculated monetary value for SOC storage in the contiguous U.S. was between $4.64T (i.e., $4.64 trillion U.S. dollars, where T = trillion = 1012) and $23.1T, with a midpoint value of $12.7T. Soil orders with the highest midpoint SOC storage values were 1): Mollisols ($4.21T), 2) Histosols ($2.31T), and 3) Alfisols ($1.48T). The midpoint values of SOC normalized by area within soil order boundaries were ranked: 1) Histosols ($21.58 m−2), 2) Vertisols ($2.26 m−2), and 3) Mollisols ($2.08 m−2). The soil depth interval with the highest midpoint values of SOC storage and content was 20–100 cm ($6.18T and $0.84 m−2, respectively), while the depth interval 100–200 cm had the lowest midpoint values of SOC storage ($2.88T) and content ($0.39 m−2). The depth trends exemplify the prominence of SOC in the upper portions of soil. The LRRs with the highest midpoint SOC storage values were: 1) M – Central Feed Grains and Livestock Region ($1.8T), 2) T – Atlantic and Gulf Coast Lowland Forest and Crop Region ($1.26T), and 3) K – Northern Lake States Forest and Forage Region ($1.16T). The midpoint values of SOC normalized by area within LRR boundaries were ranked: 1) U – Florida Subtropical Fruit, Truck Crop, and Range Region ($6.10 m−2), 2) T – Atlantic and Gulf Coast Lowland Forest and Crop Region ($5.44 m−2), and 3) K – Northern Lake States Forest and Forage Region ($3.88 m−2). States with the highest midpoint values of SOC storage were: 1) Texas ($1.08T), 2) Minnesota ($834B) (i.e., $834 billion U.S. dollars, where B = billion = 109), and 3) Florida ($742B). Midpoint values of SOC normalized by area within state boundaries were ranked: 1) Florida ($5.44 m−2), 2) Delaware ($4.10 m−2), and 3) Minnesota ($3.99 m−2). Regions with the highest midpoint values of SOC storage were: 1) Midwest ($3.17T), 2) Southeast ($2.44T), and 3) Northern Plains ($2.35T). Midpoint values of SOC normalized by area within region boundaries were ranked: 1) Midwest ($2.73 m−2), 2) Southeast ($2.31 m−2), and 3) East ($1.82 m−2). The reported values and trends demonstrate the need for policies with regards to SOC management, which requires incentives within administrative boundaries but informed by the geographic distribution of SOC.
Combining Deep Learning and Prior Knowledge for Crop Mapping in Tropical Regions from Multitemporal SAR Image Sequences
Authors: Cué La Rosa Laura Elena , Queiroz Feitosa Raul , Nigri Happ Patrick , Del’Arco Sanches Ieda , Ostwald Pedro da Costa Gilson Alexandre
Published: August 2019

Accurate crop type identification and crop area estimation from remote sensing data in tropical regions are still considered challenging tasks. The more favorable weather conditions, in comparison to the characteristic conditions of temperate regions, permit higher flexibility in land use, planning, and management, which implies complex crop dynamics. Moreover, the frequent cloud cover prevents the use of optical data during large periods of the year, making SAR data an attractive alternative for crop mapping in tropical regions. This paper evaluates the effectiveness of Deep Learning (DL) techniques for crop recognition from multi-date SAR images from tropical regions. Three DL strategies are investigated: autoencoders, convolutional neural networks, and fully-convolutional networks. The paper further proposes a post-classification technique to enforce prior knowledge about crop dynamics in the target area. Experiments conducted on a Sentinel-1 multitemporal sequence of a tropical region in Brazil reveal the pros and cons of the tested methods. In our experiments, the proposed crop dynamics model was able to correct up to 16.5% of classification errors and managed to improve the performance up to 3.2% and 8.7% in terms of overall accuracy and average F1-score, respectively.