bluuebunny/crossref_metadata_2025 huggingface dataset:
O ensino primário no governo Juscelino Kubitschek (1956-1961)
Authors: Padilha Caio Augusto Toledo
Published: October 2024

Este trabalho consiste em uma análise sócio-histórica da atuação do governo Juscelino Kubitschek (1956-1961) na educação, mais especificamente no âmbito do ensino primário, em um período marcado pelo avanço do sistema capitalista de produção. Nessa direção, a educação escolar atinge novos patamares de importância na agenda estatal, uma vez que se tornava imperativo garantir a efetivação do direito à educação e, não obstante, formar os indivíduos para o exercício da cidadania em um cenário democrático e qualificá-los para o exercício dos novos postos de trabalho que o capitalismo proporcionaria nos anos vindouros. Isto posto, foi realizado um levantamento de dados junto a fontes primárias e secundárias, que posteriormente foram sistematizados e analisados criticamente, possibilitando a identificação do que foi idealizado e efetivamente realizado pelo governo Kubitschek no âmbito do ensino primário para adequá-lo às necessidades de um país em acelerado processo de modernização econômica, cultural, política e social.
Percepción sobre prácticas sexuales e infecciones de transmisión sexual en jóvenes universitarios de Lima, Perú
Authors: Carrillo Garibay Mariafernanda , Flores Fuentes Milagros , Melgar Morán Carlos Christian
Published: October 2024

Objetivo: Determinar las percepciones sobre las prácticas sexuales e infecciones de transmisión sexual en jóvenes de la Universidad Nacional de Ingeniería, Lima, Perú. Materiales y métodos: Estudio descriptivo, con enfoque cuantitativo y de corte transversal. La muestra estuvo conformada por 361 jóvenes de cuatro carreras de Ingeniería del primer al quinto año. Se diseñó un cuestionario que fue sometido a juicio de expertos; la validez de contenido mediante la prueba binomial fue de p = 0,016; y la confiabilidad se realizó mediante prueba piloto, resultando un alfa de Cronbach de 0,783. Resultados: La mayoría de los encuestados fueron hombres (87,8 %), solteros (97,2 %) y el 63,7 % ya había iniciado su vida sexual; la edad promedio de inicio de vida sexual fue 18,2 años. Las percepciones medianamente favorables acerca de las infecciones de transmisión sexual fueron prevalentes en ambos géneros: el 77,3 % en mujeres y el 71,6 % en hombres. En cuanto a las prácticas sexuales, el 65,9 % en el sexo femenino y el 77,9 % del sexo opuesto mostraron percepciones medianamente favorables. Conclusiones: Los jóvenes de una universidad de ingeniería tienen percepciones medianamente favorables con relación a las prácticas sexuales e infecciones de transmisión sexual.
Estudio de la viabilidad del uso de carbón activado de residuos agroindustriales en un filtro electro-Fenton como alternativa al carbón activado comercial para el tratamiento de agua residual textil
Authors: Vásquez-Gómez Nancy F. , Martínez Rodrigo J. , Banda-Alemán Jorge A. , Espejel-Ayala Fabricio , García-Espinoza Josué D. , Godínez Luis A. , Robles Irma
Published: October 2024

El presente trabajo se enfocó a analizar la factibilidad de implementar carbones activados fabricados con residuos agroindustriales en un dispositivo electroquímico filtrante. El dispositivo consta de un reactor a escala de laboratorio de 90 mL empacado con carbón activado y se utilizó azul brillante FCF (AB) como contaminante modelo. Se evaluaron tres variables mediante un diseño factorial completo: precursor del carbón activado: cáscara de naranja o borra de café; modo de operación del reactor: continuo o por lotes; tipo de proceso electroquímico: electro-Fenton o electrooxidación. Las condiciones óptimas consistieron en utilizar carbón activado de cáscara de naranja y operar el proceso en continuo mediante electro-Fenton, las cuales redujeron la concentración de colorante en 40 % tras 180 min de operación. Se validaron estas condiciones en un dispositivo a escala prototipo de 425 mL tanto para el AB como en agua residual proveniente de una planta textil. Para esta última, la variable de respuesta evaluada fue la disminución de carbono orgánico total. Los resultados para el AB muestran más del 60 % de eficiencia al usar el colorante; sin embargo, la concentración de carbono orgánico total del agua residual se mantuvo constante, lo cual indica que el dispositivo aún requiere estudios complementarios para ser implementado utilizando carbones activados de origen agroindustrial.
Validation of Electromechanical Transient Model for Large-Scale Renewable Power Plant Based on Fast-Responding Generator Method
Authors: Zhao Dawei , Ning Yujie , Zhang Chuanzhi , Ma Jin , Qian Minhui , Liu Yanzhang
Published: October 2024

The requirements for accurate models of renewable energy power plant are urgent for power system operation analysis. Most existing model research in this area is for wind turbine and photovoltaic (PV) power generation unit, a rare renewable power plant model validation mainly adopts the single-machine infinite-bus system. The single equivalent machine method is always used, and the interactions between the power plant and the grid are ignored. The voltage at the interface bus is treated as constant, although this is not consistent with its actual characteristics. The phase shifter method of hybrid dynamic simulation has been applied in the model validation of wind farm. However, this method is heavily dependent on phasor measurement units (PMU) data, resulting in limited application scope, and it is difficult to realize the model error location step by step. In this paper, the fast-responding generator method is used for renewable power plant model validation. The complete scheme comprising model validation, error localization, parameter sensitivity analysis, and parameter correction is proposed. Model validation is conducted based on measured records from a large-scale PV power plant in northwest China. The comparison of simulated and measured data verifies the feasibility and accuracy of the proposed scheme.
Analyzing Impacts of Marble Industry on Public Health
Authors: Anwar Seemul
Published: October 2024

A vast amount of non-biodegradable waste is generated during mining and processing operations of dimensional stones such as marble. This, over the years has become a global nuisance by damaging the environment through means of more than one. Therefore it has become necessary to find a technique to safely dispose or reuse marble waste. This paper reviews the impact of replacement of the conventional materials by marble waste was studied in terms of fresh, mechanical and durability properties of concrete. The air pollution caused by the dust generated in the process of crushing and extracting marble can cause serious problems to the general physiological functions of plants and it affects human life as well. Therefore, the objectives of this study were to assess the air quality of areas with marble factories and areas without marble factories, where the concentration of particulate matter in terms of total suspended particles (TSP) was determined. This paper also reviews some eco-innovation options considering the pollution caused, consumption of water and electricity and the disposal of marble waste and their waste.
Baxdrostat and finerenone: new aldosterone synthase-aldosterone-mineralocorticoid receptor hormonal system inhibitors for the drug treatment of resistant arterial hypertension
Authors: Kuzmin O. B. , Buchneva N. V. , Belyanin V. V. , Zhezha V. V. , Stolbova M. V.
Published: October 2024

Resistant arterial hypertension is characterized by failure to control target blood pressure despite long-term use of optimal or maximum tolerated doses of three different antihypertensive drugs, including diuretic. Patients with resistant hypertension are included in a group of people at high risk of cardiovascular and renal complications, including accelerated progression of chronic kidney disease with a more rapid transition to the final stage of the disease. Resistant hypertension is based on a salt-sensitive, volume-dependent form of hypertension, which usually occurs against the background of increased aldosterone production and normal or even decreased renin plasma activity. A key role in its formation is played by an increase of sodium reabsorption in the kidneys, associated with excessive activity of aldosterone-sensitive epithelial sodium channels (ENaC), which control the reabsorption of this ion in the distal segments of the nephron. Its assumed that in this pathological process, in addition to aldosterone, is also involved the small Rho GTFase Rac1 — regulatory G-protein, which can enter into a direct ligand-independent interaction with mineralcorticoid receptors, performing the function of a powerful nonsteroidal activator of the transmission of their intracellular signals. Based on controlled, randomized clinical trials, the optimal fourth drug to overcome resistance in such patients is the steroid mineralcorticoid receptor antagonist spironolactone. However, the inclusion of this drug in antihypertensive therapy not only fails to control blood pressure in a significant proportion of patients with resistant hypertension, but also significantly increases the risk of hyperkalemia, especially in people with impaired renal function. The review presents data on the pharmacodynamics and pharmacokinetics of new inhibitors of aldosterone synthase-aldosterone-mineralocorticoid receptor hormonal system baxdrostat and finerenone, as well as the results of clinical studies assessing the clinical effectiveness and safety profile of these drugs in patients with resistant hypertension.
Original drugs and generics: are there any differences from the clinician’s point of view?
Authors: Martsevich S. Yu. , Kutishenko N. P. , Drapkina O. M.
Published: October 2024

The article defines the original drug (OD) and examines the history of the appearance of generic drugs (GD), as well as the evolution of views to prove their bioequivalence to OD. The question is considered to what extent pharmacokinetic equivalence can guarantee the clinical equivalence of OD and GD. The data on the rules of registration of GD in different countries are provided. A brief overview of various types of studies comparing the clinical efficacy and safety of OD and GD (meta-analyses, randomized controlled trials, observational studies, description of clinical cases) and their main results is given. The results of a number of observational studies on the replacement of OD with GD and its consequences are presented. The existing system of current quality control of GD is described, numerous cases of recall of GD due to detected violations during their production are given. It is mentioned about individual cases of clinical inefficiency of GD and their side effects identified by the practical physicians. It is concluded that a qualitatively produced GD with proven pharmacokinetic equivalence to OD is able to provide therapy of the same quality as OD. However, numerous cases of the appearance of GD on the pharmaceutical market, which does not correspond to the quality of OD, make us somewhat wary of therapy based on VP. Both the practitioner and the patient should know which of the drugs prescribed by the international nonproprietary name is OD and which is GD.
DISEÑO DE UN PICO SATÉLITE CANSAT PARA LA PREDICCIÓN DEL MONÓXIDO DE CARBONO (CO) EN LA UNIVERSIDAD LIBRE, SEDE EL BOSQUE
Authors: Lemus Casas Jaime Alfonso , Saavedra Polanco María Alejandra , Santa Quintero Ricardo Andres
Published: October 2024

This research aims to design the hardware for a CANSAT picosatellite for the prediction of carbon monoxide (CO) in the air at the Libre University El Bosque campus. The goal is to identify the necessary sensors to collect data and establish the levels of CO pollution in different areas of the mentioned university.Additionally, the defined functional and non-functional requirements necessitate a detailed analysis for the selection of sensors, control board, communication system, and power system. A requirements verification model based on use cases involving quantitative and qualitative measurements, as well as theoretical maximum and minimum values, is also required.
DOI: http://dx.doi.org/10.21017/rimci.2024.v11.n21.a150
EXPLORANDO LAS POSIBILIDADES DEL EDGE COMPUTING EN LA ERA DE LA CONECTIVIDAD
Authors: Garnica Estrada Evelyn , Guerrero Rincón Joan Sebastián
Published: October 2024

In today’s age of connectivity, Edge Computing is emerging as an innovative solution that redefines the way data is processed and analyzed. This article describes Edge Computing, exploring its possibilities and its impact on currenttechnology, addressing aspects from latency reduction to improving efficiency in data use. The advantages and challenges that this technology introduces in fields such as the Internet of Things (IoT), real-time data analytics and privacy are illustrated. With a focus on the evolution of cloud computing, this article invites readers to explore how edge computing is transforming connectivity and opening new possibilities for innovation in an increasingly interconnected world.
DOI: http://dx.doi.org/10.21017/rimci.2024.v11.n21.a160
Türk-İslam Kültüründe Söğüt Ağacı ve Osmanlı Tasvirlerinin İkonografisi Üzerine
Authors: Altıer Semiha
Published: October 2024

Çeşitli ağaç türleri fiziksel özellikleriyle bağlantılı olarak, neredeyse her toplumun kültüründe önemli bir yer tutmuştur. Bunlar içinde söğüt zarif ve esnek yapısı, meyvesiz oluşu, su kenarlarında yetişmesi ve dallarının yukarıdan aşağı doğru ahenkli salınışı gibi özellikleriyle çeşitli kültürlerde hem benzer hem farklı çağrışımlar yapmıştır. Batı dünyasında ölüm, yas, kavuşamayan aşıklar ve hüznü temsil ederken; Uzakdoğu’da kadının zerafetini, soyu, iffeti, ayrılığı; Orta Asya’da ise koruyuculuğu, şifa, şans ve bereketi çağrıştırmıştır.
Söğüt, Türk ve İslam kültüründe, halk hikayelerinde, atasözlerinde, türkülerde, ninnilerde, masallarda, manilerde ve yer adlarında, tarikat ehlinin kerametlerinde ve isimlerinde görülür. Osmanlı tasvir sanatları söz konusu olduğunda ise çoğunlukla su kenarındaki manzara, kent ve yapı görüntülerinde resimlenmiştir. Çalışmanın odak noktasını ise 19.-20. yüzyıllarda Osmanlı taşrasında tarikatların etkin olduğu bölgelerdeki yapıların duvar resimlerinde inançlara ve cennet imgesine gönderme yapan söğüt tasvirleri oluşturmuştur. Bu tasvirler, tarikat ehli olduğu anlaşılan halk ressamları ve isimleriyle bilemediğimiz yerel sanatçılar tarafından minyatür tarzında, bazı örneklerde ise dönemin natüralist anlayışına uygun yapılmışlardır.
Sınırlı sayıda olsa söğüt ağacı tasvirleri Osmanlı halk kültürünün zenginliğini görsel olarak da seyircisine sunmaktadır.
Распространение вариантов вируса SARS-COV-2, вызывающих интерес (VOI) и находящихся под наблюдением (VUM), на основе количества их геномов, депонированных в базу данных GISAID за неделю с 12 по 18 октября 2024 г.
Authors: Дмитриева Людмила , Чумачкова Елена , Краснов Ярослав , Осина Наталья , Зимирова Анастасия , Иванова Александра , Карнаухов Игорь , Караваева Татьяна , Щербакова Светлана , Кутырев Владимир
Published: October 2024

В обзоре представлена информация по циркулирующим в настоящее время вариантам вируса SARS-COV-2 Omicron вызывающих интерес (VOI) и находящихся под наблюдением (VUM), геномные последовательности которых размещены в международной базе данных GISAID за неделю с 12 по 18 октября 2024 г.
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 12.10.2024 г. по 18.10.2024 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: October 2024

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19 за неделю с 12.10.2024 г. по 18.10.2024 г.
Intra-articular Calcaneal Fracture Fixation Complicated by a Traumatic Chylous Effusion
Authors: Murray Benjamin , Serhan Karolina A. , Deafenbaugh Bradley K. , Manson H. Corey
Published: October 2024

Case:

This is a rare case describing the management of a traumatic chylous effusion discovered during operative fixation of a Sanders type III calcaneus fracture in a 58-year-old man, sustained after a fall from height. Chylous effusions are uncommon and can mimic septic arthritis. Given the concern for possible infection intraoperatively, provisional K-wire–assisted reduction was performed and definitively maintained through antibiotic mixed polymethylmethacrylate cement. Despite foregoing traditional fixation techniques, an excellent outcome was achieved.

Conclusion:

This case outlines the use of antibiotic cement as a reduction aid for an intra-articular calcaneus fracture in the setting of a traumatic chylous effusion.
BACIA ESCOLA
Authors: Kobiyama Masato , Ruoso Erika Gabriella , Campagnolo Karla , Sato Anderson Mululo , Fagundes Marina Refatti , Zambrano Fernando Campo
Published: October 2024

A crescente complexidade global exige uma gestão avançada de setores como recursos hídricos, desastres e meio ambiente, destacando abordagens baseadas em ciência, evidências, usabilidade e participação da comunidade local. Isso conecta fortemente ciência e comunidade, e ressalta a importância da ciência cidadã. Para implementar essa gestão integrada, o uso da bacia escola é essencial. Este estudo teve como objetivo desenvolver a aplicação prática da bacia escola, esclarecendo seus aspectos fundamentais por meio de análise histórica, definição do conceito, orientações para implementação, discussão da ciência cidadã e apresentação de dois estudos de caso no Brasil. A revisão bibliográfica identificou que o termo “bacia escola” foi utilizado pela primeira vez na década de 1960 no nordeste brasileiro, sendo considerado pioneiro no Brasil e no mundo. Com base nas análises, este trabalho define a bacia escola como uma bacia hidrográfica onde ocorrem monitoramento e diagnóstico em campo, utilizada para pesquisas científicas e atividades didáticas voltadas ao aprendizado de ciências, educação e formação intelectual dos cidadãos envolvidos. Devido à sua função social, a bacia escola se configura como uma unidade ideal para a hidrologia, especialmente a socio-hidrologia, promovendo a ciência cidadã e praticando a hidrossolidariedade na sociedade. Demonstrando a efetividade da bacia escola através de dois estudos de caso, este trabalho sugere a criação de um Programa Nacional de Bacias Escola no Brasil.
PROJETO ÁGUA BOA:
Authors: Fuziel Camila , Fernandes Diogenes Paulo Ricarde , Moita Porto Reny , Vieira dos Santos Júnior Fausto , Barroso Campêlo Marcellus  José , Silva do Valle Armando
Published: October 2024

Este relato de trabalho de campo aborda o Projeto Água Boa, desenvolvido no município de Careiro da Várzea-AM, com foco em ações voltadas para o abastecimento de água potável. O projeto tem beneficiado comunidades ribeirinhas, promovendo a saúde e o bem-estar da população local. Foi criado em resposta aos desafios climáticos enfrentados no Amazonas. Governo do Estado, por meio da Secretaria de Desenvolvimento Urbano e Metropolitano (SEDURB), Unidade Gestora de Projetos Especiais (UGPE) e Companhia de Saneamento do Amazonas (COSAMA), uniu esforços com o Fundo das Nações Unidas para a Infância (UNICEF), para implementar essa importante iniciativa. Em 2020, durante a pandemia de Covid-19, o projeto incluiu a instalação de três Soluções Alternativas Coletivas de Abastecimento de Água para Consumo Humano (SAC). Utilizando a Ferramenta de Análise de Gargalos no Setor de Água, Saneamento e Higiene (WASH BAT) e Avaliação Participativa dos Riscos Climáticos, foram identificadas as principais dificuldades de acesso à água potável e ao esgotamento sanitário, bem como os riscos climáticos que afetam o abastecimento no município. O projeto foi concebido para garantir água segura às populações ribeirinhas em situação de vulnerabilidade socioambiental. A metodologia adotada inclui a implementação de uma SAC, que contempla os processos de coagulação, floculação, sedimentação, filtração e desinfecção, além de infraestrutura projetada com base na resiliência a eventos climáticos extremos. O projeto também engloba a capacitação das comunidades locais e a celebração de uma cooperação com o município. O Projeto Água Boa é uma iniciativa colaborativa, que busca soluções sustentáveis e eficazes para os desafios enfrentados pelas comunidades ribeirinhas. Já beneficia cerca de 4 mil pessoas, fortalecendo a resiliência das populações locais frente aos eventos extremos intensificados pelas mudanças climáticas.
WITHDRAWN: Different Storage Conditions Affects Microbial Profiles in Chicken Cecal Chyme and Lung
Authors: Yang Jiajun , Wang Jing , Zhang Bo , Qin Shunyi , Zhang Hao , Liu Guofang
Published: October 2024

The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Metabolic rewiring in fat-depleted Drosophila reveals triglyceride:glycogen crosstalk and identifies cDIP as a new regulator of energy metabolism
Authors: Henne W. Mike , Ugrankar-Banerjee Rupali , Tran Son , Bowerman Jade , Paul Blessy , Zacharias Lauren , Mathews Thomas , DeBerardinis Ralph
Published: October 2024

Tissues store excess nutrients as triglyceride or glycogen, but how these reserves are sensed and communicate remains poorly understood. Here we identify molecular players orchestrating this metabolic balance during fat depletion. We show fat body (FB)-specific depletion of fatty acyl-CoA synthase FASN1 in Drosophila causes near-complete fat loss and metabolic remodeling that dramatically elevates glycogen storage and carbohydrate metabolism. Proteomics and metabolomics identify key factors necessary for rewiring including glycolysis enzymes and target-of-brain-insulin (tobi). FASN1-deficient flies are viable but starvation sensitive, oxidatively stressed, and infertile. We also identify CG10824/cDIP as upregulated in FASN1-depleted Drosophila. cDIP is a leucine-rich-repeat protein with homology to secreted adipokines that fine-tune energy signaling, and is required for fly development in the absence of FASN1. Collectively, we show fat-depleted Drosophila rewire their metabolism to complete development, and identify cDIP as a putative new cytokine that signals fat insufficiency and may regulate energy homeostasis.
Unlocking the Power of Source-Separated Organic Municipal Waste for Fertilizer Production and Sustainable Waste Management in Ethiopia
Authors: Ali Hussien , Leta Seyoum , Hussen Ahmed , Hassen Bantamlak , Terfie Tadesse Alemu
Published: October 2024

Purpose
This study aims to optimize the process of converting organic municipal solid waste (MSW) into organic fertilizer using a pilot Aerated Static Pile (ASP) composting system. It focuses on identifying the best waste mixing ratios, optimizing operational parameters, and evaluating the quality of the resulting fertilizer to enhance waste management in developing countries like Ethiopia, where resources are limited, and urbanization is increasing.
Methods
The study tested biodegradable organic MSW, supplemented with cow-dung and sediment inocula, in various compositions. It analyzed key composting parameters such as; pH, carbon to nitrogen (C/N) ratio, moisture content, and electrical conductivity to determine the optimal composting conditions. Additionally, heavy metal concentrations were measured before and after composting to ensure the safety of the final product.
Results
The study identified optimal composting conditions: a pH of 5.43 to 7.89, a C/N ratio of 23:1 to 42:1, moisture content of 60–66%, and electrical conductivity of 4.10–5.24%. Heavy metal concentrations remained within regulatory limits throughout the process. The optimization results showed that the natural sediment inoculum (Run I) was more efficient than the cow-dung inoculum (Run II) for decomposition. In Run I, Pile P3 (50% food waste, 50%-yard waste) produced the best quality compost, followed by Pile P2 (75% food waste, 25%-yard waste). Pile P4 (25% food waste, 75%-yard waste) had lower temperatures, hindering decomposition.
Conclusion
This research underscores the importance of optimized composting processes and provides valuable insights into implementing ASP composting technology for organic waste management in Ethiopia and beyond.
Graphical Abstract
Monitoring and assessment of polycyclic aromatic hydrocarbons in pizza samples (meat and chicken) using modified MSPE extraction and GC/MS method
Authors: Shariatifar Nabi , Arabameri Majid , Moazzen Mojtaba , Shavli Parisa , Hadian Zahra
Published: October 2024

The objective of present research was to assess the PAH (polycyclic aromatic hydrocarbon) concentrations in Pizza (chicken and beef) cooked, by technique of MSPE-GC/MS (magnetic solid phase extraction-gas chromatography mas spectrophotometry). The outcomes revealed the mean ± SD of PAH4, ∑PAHs and BaP in all pizza samples was 2.97 ± 1.82, 12.75 ± 2.1 and 0.18 ± 0.02µg/kg, respectively. Based on the present results, in meat pizza samples, the mean ± SD (min-max) of PAH4, total PAHs and BaP was 4.20 ± 0.9 14.90 ± 1.59 and 0.31 ± 0.02 µg/kg, respectively, and in chicken pizza samples was 1.5 ± 0.8 10.18 ± 1.96 and 0.03 ± 0.01, respectively. A Heat map was used to understand the individual similarities and distinctions between PAHs detected in each sample with color intensity. The Based on the Monte Carlo consequences achieved, the EDI (estimated daily intake) of PAH measured was ranked as B(k)F > Fl > B(a)P > B(b)F > B(a)A > Ph > CHR > I(1,2,3-cd)P > D(a,h)A. The ILCR (incremental lifetime cancer risk) for the children and adults was 2.28E-8 and 6.07E-9, respectively. The research’s outcomes indicate the contents of PAH in pizza (chicken and beef) do not pose a safety concern for consumers in Iran (ILCR < 10− 6).
Comprehensive analysis of the cardiac whole-transcriptome expression profiling involved in rehabilitation exercise improving myocardial remodeling after acute myocardial infarction
Authors: Chen Xueying , Chen Mingrui , Li Zhongxin , Yan Yugang , Lin Nan , Ni Liangchun , Zhang Yang , Kang Nan , Gao Haizhu , Guo Cuimei , Bian Xinxin , Zhang Shaohui , Zhang Qingyun , Gan Lijun
Published: October 2024

Rehabilitation exercise (RE) has been shown to mitigate cardiac remodeling and enhance cardiac function in patients with acute myocardial infarction (AMI). However, the precise molecular mechanisms underlying these effects remain incompletely elucidated. In this study, we established a mice model of acute myocardial infarction (AMI) and implemented an 8-week rehabilitation exercise intervention. Whole-transcription sequencing was conducted to examine the expression patterns of mRNA and non-coding RNAs (ncRNAs) in the myocardium of mice with AMI, with and without rehabilitation exercise (RE). GO, KEGG pathway enrichment and protein-protein interaction (PPI) network analyses were performed. And the mRANs within the ceRNA network were validated by RT-PCR. Our findings demonstrate that RE effectively enhances cardiac function, attenuates fibrosis and promotes angiogenesis in the myocardial tissue following MI. Furthermore, bio informatics tools and databases were utilized to investigate potential functions and associations of non-coding RNAs. Our data revealed that: a total of 100 long ncRNAs (lncRNAs), 14 microRNAs (miRNAs), 131 circular RNAs (circRNAs), and 1028 messenger RNAs (mRNAs) were significant. The most prominent pathways involved in RE-mediated improvement of cardiac remodeling after AMI are the PI3K-Akt signaling pathway, cytokine − cytokine receptor interaction, chemokine signaling pathway and MAPK signaling pathway. In addition, lncRNA‒miRNA-mRNA and circRNA-miRNA‒mRNA networks of RE-mediated improvement of cardiac remodeling after AMI were constructed. The present study elucidates the physiological roles of mRNA and ncRNAs in facilitating cardiac remodeling post-AMI. Our findings establish a theoretical framework for investigating the mechanisms underlying cardiac remodeling after AMI, while also providing valuable insights for exploring potential therapeutic interventions for AMI.
Development and validation of a prediction model for intrapartum fever related to chorioamnionitis in parturients undergoing epidural analgesia
Authors: Ling Liang , Liu Bo , Li Chunping , Zhang Dan , Jia Fei , Tang Yong , Chen Benzhen , Wang Mengqiao , Zhang Jian
Published: October 2024

Intrapartum fever is a common complication in parturients undergoing epidural analgesia(EA), significantly increasing the incidence of maternal and infant complications. This study aims to develop and validate a prediction model for intrapartum fever related to chorioamnionitis(IFTC) in parturients undergoing epidural analgesia. A total of 596 parturients with fever (axillary temperature ≥ 38℃) who received EA from January 2020 to December 2023 were included and randomly assigned to the training set (N = 417) and the validation set (N = 179) according to the ratio of 7:3. The independent risk factors were screened by univariate and multivariate logistic regression analysis to develop a nomogram model. Decision curve analysis (DCA) was used to evaluate the clinical effectiveness and discrimination of the model.;calibration curve was used to assess the accuracy of the model. Maximum temperature, meconium-stained amniotic fluid, C-reactive protein (CRP), gestational age and BMI were independent risk factors for predicting IFTC,and the area under receiver operating characteristic curve(AUC) of the training set and the validation set were 0.744 (0.691–0.796) and 0.793 (0.714–0.872), respectively. The calibration curve showed good consistency between predicted and actual results. DCA curve showed that the model had clinical value throughout a broad threshold probability range. The nomogram prediction model based on CRP, meconium-stained amniotic fluid, maximum temperature, gestational age and BMI has good predictive performance for the risk of IFTC in EA parturients.
The advisability of science fair project topic origins: a case study of learners from disadvantaged backgrounds
Authors: Stott Angela Elisabeth
Published: October 2024

For learners from disadvantaged backgrounds with limited science fair-related skills, knowledge, and community support, investigating the advisability of various ways of arriving at a science fair project topic is needed. This mixed-methods case study uses questionnaire data and science fair awards regarding the engagement of 23 grade 9-10 learners in a year-long university-led intervention. Data analysis was conducted for four post-hoc determined topic-origin groups of these learners’ projects: internet, self, adult, and adult after the learner had displayed competence. Descriptive statistics and thematic analysis were used to determine whether each topic-origin group met the advisability criteria regarding learners’ perceptions of value and facilitators’ perceptions of feasibility. Each learner who was provided with a topic by an adult without first having displayed competence through designing a project of their choosing required unfeasibly high levels of facilitator input to remain in the programme. Generally, the greatest success was observed for the learners to whom an adult gave a topic after displaying competence. These findings and how the intervention was conducted are explained in terms of the four paradigms of learning and instructional design. A framework (Instruct-Expose-Explore-Formalise) for deriving science fair topics is suggested to enhance the efficiency and effectiveness of similar interventions.
Abnormalities in Brain Complexity in Children with Autism Spectrum Disorder: A Sleeping State Functional MRI Study.
Authors: Fu Shishun , Wang Xiang , Chen Ziwei , Huang Zengfa , Feng Yin , Xie Yuanliang , Li Xiang , Yang Chunlan , Xu Shoujun
Published: October 2024

Background and Objective: The theory of complexity loss in mentally disordered brain is widely acknowledged. However, the findings of autism research do not seem to align well with this theory. We aim to investigate the brain complexity in children with ASD (Autism Spectrum Disorders) compared with the TD (Typical Developed) children in sleeping state.
Method: 42 ASD children and 42 TD children were imaged using sleep-state functional magnetic resonance imaging (ss-fMRI), and brain complexity was analyzed by employing sample entropy (SampEn) and transfer entropy (TE). For the ASD group, we also investigated the relationship of symptom severity with SampEn and with TE.
Results: In compared with TD group, ASD group showed significant elevated SampEn in the right inferior frontal gyrus. However, in the group of TD, 13 pairs of brain regions exhibit higher TE compared to the ASD group. In the ASD group, the TE of 5 pairs of brain regions is higher than in the TD group. A positive correlation was found between cerebellum-amygdala TE in ASD group and ABC total scores.
Conclusion: This sleeping-state fMRI study provide evidence that ASD children exhibited aberrant brain complexity in compare with the TD children. The complexity of the autistic brain is composed of aberrant randomness in brain activity and anomalous information transmission between brain regions. We propose that the abnormally diminished quantity of information transmission may potentially underlie the decreased complexity of the autistic brain.
Toward precision medicine: Exploring proteomic signatures in sepsis and non-infectious systemic inflammatory response syndrome
Authors: Ruiz-Sanmartín Adolfo , Ribas Vicent , Suñol David , Chiscano-Camón Luis , Martín Laura , Bajaña Iván , Bastida Juliana , Larrosa Nieves , González Juan José , Carrasco María Dolores , Canela Núria , Ferrer Ricard , Ruiz-Rodríguez Juan Carlos
Published: October 2024

Background
The search for new biomarkers that allow an early diagnosis in sepsis has become a necessity in medicine. The objective of this study is to identify potential protein biomarkers of differential expression between sepsis and non-infectious systemic inflammatory response syndrome (NISIRS).
Methods
Prospective observational study of a cohort of septic patients activated by the Sepsis Code and patients admitted with NISIRS, during the period 2016–2017. A mass spectrometry-based approach was used to analyze the plasma proteins in the enrolled subjects. Subsequently, using recursive feature elimination (RFE) classification and cross-validation with a vector classifier, an association of these proteins in patients with sepsis compared to patients with NISIRS. The protein-protein interaction network was analyzed with String software.
Results
277 patients were included (141 with sepsis and 136 with NISIRS). After performing RFE, 30 proteins (SERPINA4, ITIH1, ITIH3, SERPINA3, F12, FN1, SERPINA6, APOE, GSN, C3, SERPINF1, C5, LBP, CD14, FCN3, C6, C1RL, PRDX2, APOB, PPBP, SAA1, VWF, LRG1, AFM, BTD, ORM1, RBP4, LUM, COL1A1, CA1) demonstrated an association with sepsis compared to patients with NISIRS with an accuracy of 0.49 ± 0.035, precision of 0.967 ± 0.037, specificity of 0.910 ± 0.103, sensitivity of 0.964 ± 0.035 and an area under the curve (AUC) of 0.937. Of these PPBP, V1RL, C5, vWF and SERPINA4 have a greater association with Sepsis compared to NISIRS.
Conclusion
There are proteomic patterns associated with sepsis compared to NISIRS with different strength of association. Advances in understanding these protein changes may allow for the identification of new biomarkers or therapeutic targets in the future.
Fibroblasts derived C3 promotes the progression of experimental periodontitis through macrophage M1 polarization and osteoclast differentiation
Authors: Sun Hongchen , ren feilong , Zheng Shize , Luo Huanyu , Yu Xiaoyi , Li Xianjing , Song Shaoyi , Bu Wenhuan
Published: October 2024

Complement C3 plays a critical role in periodontitis. However, its source, role and underlying mechanisms remain unclear. In our study, by analyzing single-cell sequencing data from mouse model of periodontitis, we identified that C3 is primarily derived from periodontal fibroblasts. Subsequently, we demonstrated that C3a has a detrimental effect in ligature-induced periodontitis. C3ar-/- mice exhibited significantly less destruction of periodontal support tissues compared to wild-type mice, characterized by mild gingival tissue damage and reduced alveolar bone loss. This reduction was associated with decreased production of pro-inflammatory mediators and reduced osteoclast infiltration in the periodontal tissues. Mechanistic studies suggested that C3a could promote macrophage polarization and osteoclast differentiation. Finally, by analyzing single-cell sequencing data from the periodontal tissues of patients with periodontitis, we found that the results observed in mice were consistent with human data. Therefore, our findings clearly demonstrate the destructive role of fibroblast-derived C3 in ligature-induced periodontitis, driven by macrophage M1 polarization and osteoclast differentiation. These data strongly support the feasibility of C3a-targeted interventions for the treatment of human periodontitis.
Refractory testicular germ cell tumors are highly sensitive to the targeting of polycomb pathway demethylases KDM6A and KDM6B
Authors: Shokry Doha , Khan Mehwish W , Powell Christine , Johnson Samantha , Rennels Brayden C. , Boyd Raya I. , Sun Zhengyang , Fazal Zeeshan , Freemantle Sarah J. , Parker Maryanna H. , Vieson Miranda D. , Samuelson Jonathan P. , Spinella Michael J. , Singh Ratnakar
Published: October 2024

Testicular germ cell tumors (TGCTs) can be treated with cisplatin-based therapy. However, a clinically significant number of cisplatin-resistant patients die from progressive disease as no effective alternatives exist. Curative cisplatin therapy results in acute and life-long toxicities in the young TGCT patient population providing a rationale to decrease cisplatin exposure. In contrast to genetic alterations, recent evidence suggests that epigenetics is a major driving factor for TGCT formation, progression, and response to chemotherapy. Hence, targeting epigenetic pathways with “epidrugs” is one potential relatively unexplored strategy to advance TGCT treatment beyond cisplatin. In this report, we demonstrate for the first time that targeting polycomb demethylases KDM6A and KDM6B with epidrug GSK-J4 can treat both cisplatin-sensitive and -resistant TGCTs. While GSK-J4 had minimal effects alone on TGCT tumor growth in vivo, it dramatically sensitized cisplatin-sensitive and -resistant TGCTs to cisplatin. We validated KDM6A/KDM6B as the target of GSK-J4 since KDM6A/KDM6B genetic depletion had a similar effect to GSK-J4 on cisplatin-mediated anti-tumor activity and transcriptome alterations. Pharmacologic and genetic targeting of KDM6A/KDM6B potentiated or primed the p53-dominant transcriptional response to cisplatin, with also evidence for basal activation of p53. Further, several chromatin modifier genes, including BRD4, lysine demethylases, chromodomain helicase DNA binding proteins, and lysine methyltransferases, were repressed with cisplatin only in KDM6A/KDM6B-targeted cells, implying that KDM6A/KDM6B inhibition sets the stage for extensive chromatin remodeling of TGCT cells upon cisplatin treatment. Our findings demonstrate that targeting polycomb demethylases is a new potent pharmacologic strategy for treating cisplatin resistant TGCTs that warrants clinical development.
Effects of rapid enteral feed advancement with early human milk fortification in
preterm infants > 30 weeks gestation in a resource limited setting- a randomized
controlled trial
Authors: Haribalakrishna Anitha , Bhakar Ganesh , Balasubramanian Haribalakrishna , Vijayanathan Muthu , Sriniva Lakshmi
Published: October 2024

Objective: To study the effects of rapid enteral feed advancement with early feed fortification in stable very low birth weight (VLBW) infants >30 weeks gestation.
Study design: Preterm infants (N=92) were randomized to a) rapid feed advancement-early fortification - REF group (enteral feed advanced at 25-30ml/kg/day, fortification at 50ml/kg/day) or b) slow feed advancement-late fortification-SLF group (feed advanced at 15-20ml/kg/day, fortification at 100ml/kg/day).
Results: The primary outcome-time to regain birth weight was significantly lower in REF group (9 days vs 13 days, P=0.02). REF group reached full enteral feeds earlier (6 days vs 9 days, P=0.001), had lower rates of sepsis (13% vs 38%, P=0.007) and shorter hospital stay (10 days vs 15 days, P=0.01). At one year, the median Z-scores for weight [-1.5 vs -2.2, P<0.001] and head circumference [1.1 vs 0, P<0.001) were significantly higher in the REF group.
Conclusions: In VLBW infants >30 weeks, rapid feed advancement with early fortification resulted in early postnatal regain of birth weight with positive effects on growth at one year.
The Immune System’s Role in Glymphatic System Clearance and Alzheimer’s Disease Risk: A Csf Proteome-wide Study
Authors: Cullell Natalia , Caruana Giovanni , Elias Andrea , Delgado-Sanchez Ariane , Artero Cristina , Buongiorno Maria Teresa , Almería Marta , Ray Nicola J , Correa Sonia A.L. , Krupinski Jerzy
Published: October 2024

Background: The emerging evidence of the role of the glymphatic system (GS) in Alzheimer’s disease (AD) provides new opportunities for intervention from the earliest stages of the disease. The aim of the study is to evaluate the efficacy of GS in AD to identify new disease biomarkers.
Methods: We performed a two-stage proteomic study to evaluate the GS health using intravenous gadolinium-based contrast agent (GBCA) with serial T1 3T magnetic resonance imaging (MRI) in individuals with amnestic mild cognitive impairment (a-MCI). In Stage 1 (evaluated in the Cohort 1 of a-MCI participants (n=11)), we correlated the levels of 7K CSF proteins (estimated by SOMAscan) with GS health in 78 Freesurfer-segmented brain regions of interest (ROIs).
Results: A total of 173 different proteins were significantly associated with GS health (p-value < 10-3). The stronger correlations were identified for GRAAK, NSUN6, SHPS1 and TIM-4. A pathway enrichment analysis revealed that the proteins associated with GS health were mainly implicated in neurodegenerative processes, immunity and inflammation. In Stage 2, we validated these proteomic results in a new cohort of a-MCI participants (with and without evidence of AD pathology in CSF (aMCI(-) and aMCI(+); n = 22 and 7, respectively) and healthy controls (n = 10). Proteomic prediction models were generated in each ROI. These were compared with demographic-only models for identifying participants with aMCI(-) and aMCI(+) vs controls. This analysis was repeated to determine if the models could identify those with aMCI(+) from both aMCI(-) and controls. The proteomic models were found to outperform the demographic-only models.
Conclusions: Our study identifies proteins linked with GS health and involved the immune system in a-MCI participants.
Comprehensive upper-limb human-machine interaction strategies for post-stroke upper-limb rehabilitation
Authors: Xia Kang , Chang Xue-Dong , Liu Chong-Shuai , Sun Han , Wang Yi-Min , Wang Xin-Wei
Published: October 2024

Background
Stroke and its related complications, place significant burdens on human society in the 21st century, and lead to substantial demands for rehabilitation. To fulfill rehabilitation needs, human-machine interaction (HMI) technology strives continuously. Traditional passive HMI strategy requires device to be dynamically well-fitting, and executes command precisely. Advanced active HMI strategy also demands the device to react fast and accurately based on user’s intention. However, current study usually focuses on limited aspect of HMI, a complete HMI study which addresses the complexity of stroke related complications and provide the possibility for personalized post-stroke upper-limb rehabilitation is lacking.
Method
An Up-limb Rehabilitation Device and Utility System (UarDus) is proposed along with 3 HMI strategies namely robot-in-charge, therapist-in-charge and patient-in-charge. Based on physiological structure of human upper-limb and scapulohumeral rhythm (SHR) of shoulder, a base exoskeleton with 14 degrees of freedoms (DoFs) is designed as foundation of the 3 strategies. Passive robot-in-charge and therapist-in-charge strategies provides fully-assisted rehabilitation options. The active patient-in-charge strategy incorporates data acquisition matrices and a new deep learning model, which is developed based on CNN and Transformer structure, aims to provide partially-assisted rehabilitation.
Results
Kinematically, the work space of the base exoskeleton is presented first. Utilizing motion capture technology, the GH center of both human and exoskeleton is compared the well-matched curves suggesting comfortable dynamic wear experience. For robot-in-charge and therapist-in-charge strategy, the desired and measured angle-time curve present good correlation, with low phase difference, which serve the purpose of real-time control. Featuring the patient-in-charge strategy, Kernel Density Estimation (KDE) result suggesting reasonable sensor-machine-human synergy. Applying K-fold (K = 10) cross-validation method, the classification accuracy of the proposed model achieves an average of 99.6% for the designated 15 actions. The proposed model is also evaluated on public datasets, demonstrating outstanding response time and accuracy comparing with state-of-art models.
Conclusions
A validated exoskeleton hardware system named UarDus is constructed, along with the 3 HMI strategies proposed. This work provides possibility for people with stroke to engage in complex personalized rehabilitation training with dynamic comfortable wear experience.
Levodopa responsive parkinsonism in Spinocerebellar ataxia 27B
Authors: El-Masri Shaddy , Wijaithum Piyanit , Chugh Pooja , Te Joshua , Triplett James
Published: October 2024

Background:
Spinocerebellar ataxia 27B (SCA27B) is a recently characterised cause of individuals presenting with late onset cerebellar ataxia. Here, we present a case of late onset cerebellar ataxia with associated Parkinsonism that is responsive to levodopa.
Case presentation:
A 65 year-old female presents with a progressive deterioration in her gait secondary to truncal ataxia since the age of 62 years. Throughout the course of her illness, she developed asymmetrical parkinsonism, bowel and bladder incontinence, and had a background of parasomnias preceding her presentation and also a family history of late onset parkinsonism and early onset cognitive impairment. Brain magnetic resonance imaging revealed atrophy of the midbrain, PONS, and middle cerebellar peduncles. Genetic testing confirmed a pathogenic triplet repeat within the FGF14 gene consistent with a diagnosis of SCA27B. Levodopa was commenced for her parkinsonism, with improvement in the speed and amplitude of her movements, and restoration of partial independence.
Conclusion
SCA27B should be considered in individuals presenting with late onset ataxia, and may mimic alternate neurodegenerative conditions. This case report demonstrates that associated parkinsonism may be responsive to levodopa.