bluuebunny/crossref_metadata_2025 huggingface dataset:
Magnesium Doped Hydroxyapatite-Based Coatings Obtained by Pulsed Galvanostatic Electrochemical Deposition with Adjustable Electrochemical Behavior
Authors: Vranceanu Diana Maria , Ionescu Ionut Cornel , Ungureanu Elena , Cojocaru Mihai Ovidiu , Vladescu Alina , Cotrut Cosmin Mihai
Published: July 2020

The aim of this study was to adapt the electrochemical behavior in synthetic body fluid (SBF) of hydroxyapatite-based coatings obtained by pulsed galvanostatic electrochemical deposition through addition of Mg in different concentrations. The coatings were obtained by electrochemical deposition in a typical three electrodes electrochemical cell in galvanic pulsed mode. The electrolyte was obtained by subsequently dissolving Ca(NO3)2·4H2O, NH4H2PO4, and Mg(NO3)2·6H2O in ultra-pure water and the pH value was set to 5. The morphology consists of elongated and thin ribbon-like crystals for hydroxyapatite (HAp), which after the addition of Mg became a little wider. The elemental and phase composition evidenced that HAp was successfully doped with Mg through pulsed galvanostatic electrochemical deposition. The characteristics and properties of hydroxyapatite obtained electrochemically can be controlled by adding Mg in different concentrations, thus being able to obtain materials with different properties and characteristics. In addition, the addition of Mg can lead to the control of hydroxyapatite bioactive ceramics in terms of dissolution rate.
Advanced Coatings for Buildings
Authors: Maury-Ramírez Aníbal , Flores-Colen Inês , Kanematsu Hideyuki
Published: July 2020

Based on five Special Issues in Coatings, this e-book contains a series of fifteen articles demonstrating actual perspectives and new trends in advanced coatings in buildings. Innovative materials and multiperformance solutions provide a basis, contributing also to better protection of buildings’ surfaces during the service life, and users’ wellbeing.
A Multi-Scale Feature Fusion Method Based on U-Net for Retinal Vessel Segmentation
Authors: Yang Dan , Liu Guoru , Ren Mengcheng , Xu Bin , Wang Jiao
Published: July 2020

Computer-aided automatic segmentation of retinal blood vessels plays an important role in the diagnosis of diseases such as diabetes, glaucoma, and macular degeneration. In this paper, we propose a multi-scale feature fusion retinal vessel segmentation model based on U-Net, named MSFFU-Net. The model introduces the inception structure into the multi-scale feature extraction encoder part, and the max-pooling index is applied during the upsampling process in the feature fusion decoder of an improved network. The skip layer connection is used to transfer each set of feature maps generated on the encoder path to the corresponding feature maps on the decoder path. Moreover, a cost-sensitive loss function based on the Dice coefficient and cross-entropy is designed. Four transformations—rotating, mirroring, shifting and cropping—are used as data augmentation strategies, and the CLAHE algorithm is applied to image preprocessing. The proposed framework is tested and trained on DRIVE and STARE, and sensitivity (Sen), specificity (Spe), accuracy (Acc), and area under curve (AUC) are adopted as the evaluation metrics. Detailed comparisons with U-Net model, at last, it verifies the effectiveness and robustness of the proposed model. The Sen of 0.7762 and 0.7721, Spe of 0.9835 and 0.9885, Acc of 0.9694 and 0.9537 and AUC value of 0.9790 and 0.9680 were achieved on DRIVE and STARE databases, respectively. Results are also compared to other state-of-the-art methods, demonstrating that the performance of the proposed method is superior to that of other methods and showing its competitive results.
Wave–Particle–Entanglement–Ignorance Complementarity for General Bipartite Systems
Authors: Wu Wei , Wang Jin
Published: July 2020

Wave–particle duality as the defining characteristic of quantum objects is a typical example of the principle of complementarity. The wave–particle–entanglement (WPE) complementarity, initially developed for two-qubit systems, is an extended form of complementarity that combines wave–particle duality with a previously missing ingredient, quantum entanglement. For two-qubit systems in mixed states, the WPE complementarity was further completed by adding yet another piece that characterizes ignorance, forming the wave–particle–entanglement–ignorance (WPEI) complementarity. A general formulation of the WPEI complementarity can not only shed new light on fundamental problems in quantum mechanics, but can also have a wide range of experimental and practical applications in quantum-mechanical settings. The purpose of this study is to establish the WPEI complementarity for general multi-dimensional bipartite systems in pure or mixed states, and extend its range of applications to incorporate hierarchical and infinite-dimensional bipartite systems. The general formulation is facilitated by well-motivated generalizations of the relevant quantities. When faced with different directions of extensions to take, our guiding principle is that the formulated complementarity should be as simple and powerful as possible. We find that the generalized form of the WPEI complementarity contains unequal-weight averages reflecting the difference in the subsystem dimensions, and that the tangle, instead of the squared concurrence, serves as a more suitable entanglement measure in the general scenario. Two examples, a finite-dimensional bipartite system in mixed states and an infinite-dimensional bipartite system in pure states, are studied in detail to illustrate the general formalism. We also discuss our results in connection with some previous work. The WPEI complementarity for general finite-dimensional bipartite systems may be tested in multi-beam interference experiments, while the second example we studied may facilitate future experimental investigations on complementarity in infinite-dimensional bipartite systems.
Supported and Suspended 2D Material-Based FET Biosensors
Authors: Masurkar Nirul , Varma Sundeep , Mohana Reddy Arava Leela
Published: July 2020

Field Effect Transistor (FET)-based electrochemical biosensor is gaining a lot of interest due to its malleability with modern fabrication technology and the ease at which it can be integrated with modern digital electronics. To increase the sensitivity and response time of the FET-based biosensor, many semiconducting materials have been categorized, including 2 dimensional (2D) nanomaterials. These 2D materials are easy to fabricate, increase sensitivity due to the atomic layer, and are flexible for a range of biomolecule detection. Due to the atomic layer of 2D materials each device requires a supporting substrate to fabricate a biosensor. However, uneven morphology of supporting substrate leads to unreliable output from every device due to scattering effect. This review summarizes advances in 2D material-based electrochemical biosensors both in supporting and suspended configurations by using different atomic monolayer, and presents the challenges involved in supporting substrate-based 2D biosensors. In addition, we also point out the advantages of nanomaterials over bulk materials in the biosensor domain.
Synergies between e-Mobility and Photovoltaic Potentials—A Case Study on an Urban Medium Voltage Grid
Authors: Vopava Julia , Bergmann Ulrich , Kienberger Thomas
Published: July 2020

To reduce CO2 emissions, it is necessary to cover the increasing energy demand of e-mobility with renewable energy sources. Therefore, the influence of increasing e-mobility and synergy effects between e-mobility and renewable energy sources need to be investigated. The case study presented here shows results from the analysis of grid-side and energetic synergy effects between e-mobility charged only at work and photovoltaic (PV) potentials. The basis of the grid study is a simplified cell-based grid model. Following the determination of synthetic charging profiles for e-mobility, PV potential profiles, load and production profiles, we perform load flow calculations for different scenarios and a simulation period of one year using the grid model. After the grid study, the energy analyses are carried out using four key performance indicators. The grid study shows that line overloads caused by PV production are only reduced and not avoided by increasing e-mobility and vice versa. The increase in the power peak of e-mobility, by shifting the charging processes into the peak of PV potentials, leads to a reduction of the production surplus in summer, while in winter the line utilisation increases. By modelling PV potentials on real irradiation and temperature data, the investigation of key performance indicators can identify not only seasonal fluctuations but also daily fluctuations.
Effect of Diesel Fuel-Coconut Oil-Butanol Blends on Operational Parameters of Diesel Engine
Authors: Čedík Jakub , Pexa Martin , Holúbek Michal , Aleš Zdeněk , Pražan Radek , Kuchar Peter
Published: July 2020

The global concentration of greenhouse gasses in the atmosphere is increasing as well as the emissions of harmful pollutants. Utilization of liquid biofuels in combustion engines helps to reduce these negative effects. For diesel engines, the most common alternative fuels are based on vegetable oils. Blending neat vegetable oils with diesel and/or alcohol fuels is a simple way to make them suitable for diesel engines. In this study, coconut oil was used in ternary fuel blends with diesel and butanol. Coconut oil is a potentially usable source of renewable energy, especially in the Pacific, where it is a local product. Diesel fuel-coconut oil-butanol fuel blends were used in concentrations of 70%/20%/10% and 60%/20%/20%, and 100% diesel fuel was used as a reference. The effect of the fuel blends on the production of harmful emissions, engine smoke, performance parameters, fuel consumption and solid particles production was monitored during the measurement. The engine was kept at a constant speed during the measurement and the load was selected at 50%, 75% and 100%. From the results, it can be stated that in comparison with diesel fuel, specific fuel consumption increased with a positive effect on the reduction of engine smoke.
Evaluation of Filtered Spark Gap on the Lightning Protection of Distribution Transformers: Experimental and Simulation Study
Authors: Pourakbari-Kasmaei Mahdi , Mahmood Farhan , Krbal Michal , Pelikan Ludek , Orságová Jaroslava , Toman Petr , Lehtonen Matti
Published: July 2020

Protection of transformers, as one of the most expensive equipment in the power system, against lightning overvoltage impulses is a vital task. This paper, for the first time so far, investigates the effects of a filtered spark gap on the protection level of transformers against lightning overvoltage impulses. The filter is an inductor that is placed in series with the transformer and before the spark gap aiming to reduce the voltage at the connection point of the spark gap, and hence, enhancing the protection level of the transformer under lightning overvoltages. The experimental laboratory tests are accomplished on a 400 kVA, 22/0.4 kV, Delta-Star ( Δ − Y ) connection type transformer under 110 kV, and 125 kV overvoltage impulses, whereas the size of the spark gap is set to 80 mm and two inductors of 35 μ H and 119 μ H are considered. In order to perform a more in-depth analysis, a model that works reasonably close to the empirical case is developed in the EMTP-RV software. An optimization algorithm is used to determine the sensitive parameters of the double-exponential function, which is used to reproduce the applied laboratory lightning impulse voltages in the EMTP-RV environment. Moreover, the transformer is modeled according to the Cigre Guidelines (Working Group 02 of Study Committee 33). The behavior of the spark gap is simulated as close as the practical situation using the disruptive effect method. The preciseness of the simulated filtered spark gap model is verified by comparing the results of the simulated model in the EMTP-RV with the results of experimental tests. After verifying the model, different sizes of inductors are studied in the EMTP-RV environment to investigate whether larger or smaller inductors provide better protection for the transformer under lightning conditions. A comparison is performed among the conventional spark gap, surge arrester, and the filtered spark gap to provide a better analysis of the potential of the proposed device. The results indicate that proper sizing of the inductor, within an effective range, slightly enhances the protection level of the transformer.
Understanding Turn-On Transients of SiC High-Power Modules: Drain-Source Voltage Plateau Characteristics
Authors: Zhang Maosheng , Ren Na , Guo Qing , Sheng Kuang
Published: July 2020

The SiC (silicon carbide) high-power module has great potential to replace the IGBT (insulated gate bipolar transistor) power module in high-frequency and high-power applications, due to the superior properties of fast switching and low power loss, however, when the SiC high-power module operates under inappropriate conditions, the advantages of the SiC high-power module will be probably eliminated. In this paper, four kinds of SiC high-power modules are fabricated to investigate fast switching performance. The variations in characteristics of drain-source voltage at turn-on transient under the combined conditions of multiple factors are studied. A characteristic of voltage plateau is observed from the drain-source voltage waveform at turn-on transient in the experiments, and the characteristic is reproduced by simulation. The mechanism behind the voltage plateau is studied, and it is revealed that the characteristic of drain-source voltage plateau is a reflection of the miller plateau effect of gate-source voltage on drain-source voltage under the combined conditions of fast turn-on speed and low DC bus voltage, while the different values of drain-source voltage plateau are attributed to the discrepancy of structure between upper-side and lower-side in the corresponding partial path of the drain circuit loop inside the module, with the standard 62 mm package outline.
Decarbonization of Australia’s Energy System: Integrated Modeling of the Transformation of Electricity, Transportation, and Industrial Sectors
Authors: Aboumahboub Tino , Brecha Robert J. , Shrestha Himalaya Bir , Fuentes Ursula , Geiges Andreas , Hare William , Schaeffer Michiel , Welder Lara , Gidden Matthew J.
Published: July 2020

To achieve the Paris Agreement’s long-term temperature goal, current energy systems must be transformed. Australia represents an interesting case for energy system transformation modeling: with a power system dominated by fossil fuels and, specifically, with a heavy coal component, there is at the same time a vast potential for expansion and use of renewables. We used the multi-sectoral Australian Energy Modeling System (AUSeMOSYS) to perform an integrated analysis of implications for the electricity, transport, and selected industry sectors to the mid-century. The state-level resolution allows representation of regional discrepancies in renewable supply and the quantification of inter-regional grid extensions necessary for the physical integration of variable renewables. We investigated the impacts of different CO2 budgets and selected key factors on energy system transformation. Results indicate that coal-fired generation has to be phased out completely by 2030 and a fully renewable electricity supply achieved in the 2030s according to the cost-optimal pathway implied by the 1.5 °C Paris Agreement-compatible carbon budget. Wind and solar PV can play a dominant role in decarbonizing Australia’s energy system with continuous growth of demand due to the strong electrification of linked energy sectors.
Numerical Methods for Optimization of the Horizontal Directional Drilling (HDD) Well Path Trajectory
Authors: Wiśniowski Rafał , Łopata Paweł , Orłowicz Grzegorz
Published: July 2020

Advances in the field of material engineering, computerization, automation, and equipment miniaturization enable modernization of the existing technologies and development of new solutions for construction, inspection, and renovation of underground pipelines. Underground pipe installations are used in the energy sector, gas industry, telecommunications, water and sewage transport, heating, chemical industry, and environmental engineering. In order to build new pipeline networks, dig and no-dig techniques are used. Horizontal Directional Drilling (HDD) is one of the most popular trenchless technologies. The effectiveness of HDD technology application is mostly determined by its properly designed trajectory. Drilling failures and complications, which often accompany the application of the HDD technology, result from poor design of the well path in relation to the existing geological and drilling conditions. The article presented two concepts of Horizontal Directional Drilling well path trajectory design: Classic sectional, which is a combination of straight and curvilinear sections, and a single-section chain curve trajectory (catenary). Taking into account the advantages and disadvantages of the catenary trajectory relative to the sectional trajectory, the author’s solution was presented, which was the implementation of the sectional trajectory with a maximum shape similarity to the catenary trajectory. The new approach allowed us to take advantage of a chain curve trajectory and was easier to implement using the available technology. The least squares method, based on deviations from a catenary trajectory, was set as the matching criterion. The process of searching for a trajectory, being a combination of straight and curvilinear sections as similar as possible to a catenary-type trajectory, was carried out using two methodologies: State space search and a genetic algorithm. The article shows the pros and cons of both optimization methodologies. Taking into account the technical and technological limitations of HDD drilling devices, a new approach was proposed, combining the methodology of state space search with the genetic algorithm. A calculation example showed the application of the proposed methodology in an engineering design process.
Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
Authors: Zhang Shuai , Hou Yuyong , Liu Zhiyong , Ji Xiang , Wu Di , Wang Weijie , Zhang Dongyuan , Wang Wenya , Chen Shulin , Chen Fangjian
Published: July 2020

Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of Nannochloropsis oceanica IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO4 in a 16.4 mA·cm−2 current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation.
Modern Power System Dynamics, Stability and Control
Authors: Alexandridis Antonio T.
Published: July 2020

This Special Issue of Energies, “Modern Power System Dynamics, Stability and Control”, addresses the core problem of deploying novel aspects in the analysis of modern power systems as these are composed after the high penetration of distributed generation (DG) with different renewable energy sources (RES). The focus is given either on the new whole power and control system configuration or on individual cases of DG sources, power converters and other general or specific plants and devices. The problem can be tackled with different methodologies and may have several, more or less valuable and complicated solutions. The twenty-three accepted papers certainly offer a good contribution in a wide range of applications; they are extended from basic system theory perspectives, fundamental nonlinear analysis tools and novel modeling deployments to some interesting particular system and control issues.
Oak Taproot Growth Disruption Differentially Impacts Root Architecture during Nursery Production
Authors: Hankin Shanon , Watson Gary
Published: July 2020

For urban trees with strong taproots, a shift in root growth towards increased lateral root development could improve tree performance in compacted, poorly drained urban soils. In effort to achieve this desired shift, various propagation and production practices exist within the nursery industry. However, the effectiveness of practices used to disrupt taproot development, as well as their impact on root architecture, has been largely undocumented. To determine how seedling root systems respond to taproot growth disruption, we pruned oak seedling taproots either mechanically at 5 and/or 15 cm, or via air pruning at 15 cm. Taproot regeneration and lateral root development were evaluated after two years. Taproot pruning resulted in multiple regenerated taproots. The location and number of times the taproot(s) was pruned did not appear to alter the ultimate number. Mechanical taproot pruning did not affect lateral root development above the first pruning cut location at 5 or 15 cm, but generally increased the density of lateral roots below the pruning cut, likely due to the multiple taproots present. Most lateral roots were fine roots less than 1 mm in diameter (fine roots), being unlikely to become long-lived components of the root system architecture. The average number of lateral roots on air pruned (AP) seedlings was generally greater than on the same taproot segment of control (C) seedlings. To determine how these seedling changes impact the root regeneration of liner stock, we planted both taproot pruned and taproot air pruned seedlings in in-ground fabric bags filled with field soil (B) or directly into the field without bags (F). Root regeneration potential (RRP) at the bottom and lateral surfaces of the root ball were evaluated. There was less RRP on the lateral surface of the root ball in taproot air pruned, container-grown (CG) compared to taproot pruned, bare root (BR) bur oak liners, and there was no difference in red oak liners. The multiple taproots of mechanically pruned BR seedlings did not result in excessive taproot development as liners. In contrast, CG seedling taproots restricted by air pruning produced more regenerated taproots after transplanting. While seedling taproot growth disruption does disrupt the growth of a dominant single taproot and alters the architecture toward increasing the number of lateral roots, these practices do not result in laterally dominated root architecture at the liner stage of nursery production. Future research should determine how these production methods effect lateral root growth after a tree is established in the landscape and determine appropriate combinations of production methods for different species.
Pine Straw Raking and Growth of Southern Pine: Review and Recommendations
Authors: Dickens David , Morris Lawrence , Clabo David , Ogden Lee
Published: July 2020

Pine straw, the uppermost forest floor layer of undecayed, reddish-brown pine needles, is raked, baled, and sold as a landscaping mulch throughout the southeastern United States. Loblolly (Pinus taeda, L.), longleaf (P. palustris, Mill.), and slash (P. elliottii Engelm. var. elliottii) pine are the three southern pine species commonly raked for pine straw. The value of pine straw as a forest product is large. Private landowner pine straw revenues have steadily increased throughout the southeastern United States over the past two decades and now total more than USD 200 million. Information is limited on the short- or long-term effects of pine straw removal on foliage production or stand growth in southern pine stands. Results from most published studies suggest that annual pine straw raking without fertilization on non-old-field sites reduces straw yields compared to no raking. Old-field sites often do not benefit from fertilization with increased pine straw or wood volume yields. Though fertilization may be beneficial for pine straw production on some sites, understory vegetation presence and disease prevalence may increase following fertilization. This review addresses pine straw removal effects on pine straw production and stand growth parameters based on recent studies and provides fertilization recommendations to maintain or improve pine straw production and stand growth and yield.
Sequencing and Analysis of the Sex Determination Region of Populus trichocarpa
Authors: Zhou Ran , Macaya-Sanz David , Schmutz Jeremy , Jenkins Jerry W. , Tuskan Gerald A. , DiFazio Stephen P.
Published: July 2020

The ages and sizes of a sex-determination region (SDR) are difficult to determine in non-model species. Due to the lack of recombination and enrichment of repetitive elements in SDRs, the quality of assembly with short sequencing reads is universally low. Unique features present in the SDRs help provide clues about how SDRs are established and how they evolve in the absence of recombination. Several Populus species have been reported with a male heterogametic configuration of sex (XX/XY system) mapped on chromosome 19, but the exact location of the SDR has been inconsistent among species, and thus far, none of these SDRs has been fully assembled in a genomic context. Here we identify the Y-SDR from a Y-linked contig directly from a long-read PacBio assembly of a Populus trichocarpa male individual. We also identified homologous gene sequences in the SDR of P. trichocarpa and the SDR of the W chromosome in Salix purpurea. We show that inverted repeats (IRs) found in the Y-SDR and the W-SDR are lineage-specific. We hypothesize that, although the two IRs are derived from the same orthologous gene within each species, they likely have independent evolutionary histories. Furthermore, the truncated inverted repeats in P. trichocarpa may code for small RNAs that target the homologous gene for RNA-directed DNA methylation. These findings support the hypothesis that diverse sex-determining systems may be achieved through similar evolutionary pathways, thereby providing a possible mechanism to explain the lability of sex-determination systems in plants in general.
Synthetic Pesticides Used in Agricultural Production Promote Genetic Instability and Metabolic Variability in Candida spp.
Authors: Potocki Leszek , Baran Aleksandra , Oklejewicz Bernadetta , Szpyrka Ewa , Podbielska Magdalena , Schwarzbacherová Viera
Published: July 2020

The effects of triazole fungicide Tango® (epoxiconazole) and two neonicotinoid insecticide formulations Mospilan® (acetamiprid) and Calypso® (thiacloprid) were investigated in Candida albicans and three non-albicans species Candida pulcherrima, Candida glabrata and Candida tropicalis to assess the range of morphological, metabolic and genetic changes after their exposure to pesticides. Moreover, the bioavailability of pesticides, which gives us information about their metabolization was assessed using gas chromatography-mass spectrophotometry (GC-MS). The tested pesticides caused differences between the cells of the same species in the studied populations in response to ROS accumulation, the level of DNA damage, changes in fatty acids (FAs) and phospholipid profiles, change in the percentage of unsaturated to saturated FAs or the ability to biofilm. In addition, for the first time, the effect of tested neonicotinoid insecticides on the change of metabolic profile of colony cells during aging was demonstrated. Our data suggest that widely used pesticides, including insecticides, may increase cellular diversity in the Candida species population-known as clonal heterogeneity-and thus play an important role in acquiring resistance to antifungal agents.
Lessons from South Korea Regarding the Early Stage of the COVID-19 Outbreak
Authors: Chang Min Cheol , Baek Jong Hyun , Park Donghwi
Published: July 2020

South Korea has experienced difficulty in controlling the spread of the novel coronavirus disease (COVID-19) during the early stages of the outbreak. South Korea remains passionately determined to protect Koreans against COVID-19 and through trial and error hopes to improve the strategies used to limit the outbreak. Here, we review how COVID-19 spread and what prevention strategies were implemented during the early stages of the outbreak in South Korea. We investigated online newspapers published in South Korea from 21 January 2020 to 20 March 2020, and reviewed academic medical articles related to COVID-19. Additionally, we acquired data on COVID-19 cases through the official website for COVID-19 in South Korea. To date, numerous measures have been applied by the government and the medical community during the early stages of the COVID-19 outbreak including the reporting of methods for diagnostic testing, patient classification, the introduction of drive-through screening centers, COVID-19 preventive measures, implementation of government policies for the shortage of face masks, and entry restrictions. Here, we present data from the early stages of the COVID-19 outbreak and measures to prevent its spread in South Korea. We believe that sharing the experience of South Korea during the COVID-19 outbreak can help other countries to implement strategies to prevent its rapid transmission.
Dietary Acid-Base Balance in High-Performance Athletes
Authors: Baranauskas Marius , Jablonskienė Valerija , Abaravičius Jonas Algis , Samsonienė Laimutė , Stukas Rimantas
Published: July 2020

Physical exercise leads to metabolic changes that affect the acid-base balance in skeletal muscles and other tissues. Nutrition is one of the factors that may influence the acid-base balance in the body. Keeping alkaline circumstances in the body is important not only for health and athletic performance in training but also during competition in many sport events. This is especially significant for athletes who practice in sport at the highest level of competition. The aim of the study was to determine the dietary acid-base balance in competitive Lithuanian high-performance athletes, and to evaluate the effect of actual diets of athletes on NEAP (net endogenous acid production), muscle mass and body mineral content during a four-year Olympic cycle. The research participants were 18.1 ± 3.3-year-old Lithuanian high performance athletes (n = 323). The actual diet was investigated using the 24 h recall dietary survey method. The measurements of body composition were performed using BIA (bioelectrical impedance analysis). The potential renal acid load of the diets of athletes (dietary PRAL) and NEAP were calculated. In 10.2% of athletes, NEAP exceeds 100 mEq · day−1 and is on average 126.1 ± 32.7 mEq · day−1. Higher NEAP in athletes is associated with lower muscle mass (β -1.2% of body weight, p < 0.001) but has no effect on the amount of minerals in the body (β 0.01% of body weight, p = 0.073). Overall, 25–30% of Lithuanian high-performance athletes use high-protein diets (2.0–4.8 g · kg−1 · day−1) leading to a dietary acid-base imbalance as well as an excessive production of endogenous acids in the body. Athletes are recommended to consume higher amounts of potassium and magnesium. An increase in calcium intake up to 1500 mg per day is recommended. In exceptional cases, periodised nutrition for athletes may involve diets complemented with bicarbonate and/or beta-alanine supplements.
Occupational ALARA Planning for Reactor Pressure Vessel Dismantling at Kori Unit 1
Authors: Kim Juyoul , Tseren Batbuyan
Published: July 2020

Assessing workers’ safety and health during the decommissioning of nuclear power plants (NPPs) is an important procedure in terms of occupational radiation exposure (ORE). Optimizing the radiation exposure through the “As Low As Reasonably Achievable (ALARA)” principle is a very important procedure in the phase of nuclear decommissioning. Using the VISIPLAN 3D ALARA planning tool, this study aimed at assessing the radiological doses to workers during the dismantling of the reactor pressure vessel (RPV) at Kori NPP unit 1. Fragmentation and segmentation cutting processes were applied to cut the primary component. Using a simulation function in VISIPLAN, the external exposure doses were calculated for each work operation. Fragmentation involved 18 operations, whereas segmentation comprised 32 operations for each fragment. Six operations were additionally performed for both hot and cold legs of the RPV. The operations were conducted based on the radioactive waste drum’s dimensions. The results in this study indicated that the collective doses decreased as the components were cut into smaller segments. The fragmentation process showed a relatively higher collective dose compared to the segmentation operation. The active part of the RPV significantly contributed to the exposure dose and thus the shielding of workers and reduced working hours need to be considered. It was found that 60Co contained in the stainless steel of the reactor vessel greatly contributed to the dose as an activation material. The sensitivity analysis, which was conducted for different cutting methods, showed that laser cutting took a much longer time than plasma cutting and contributed higher doses to the workers. This study will be helpful in carrying out the occupational safety and health management of decommissioning workers at Kori NPP unit 1 in the near future.
Sustainable Tourism as a Source of Healthy Tourism
Authors: Santos-Roldán Luna , Castillo Canalejo Ana Mª , Berbel-Pineda Juan Manuel , Palacios-Florencio Beatriz
Published: July 2020

Even though the World Tourism Organization described Sustainable Tourism as a tourism form that could contribute to the future survival of the industry, the current reality is quite different, since it has not been firmly established in society at expected levels. The present study analyzes which variables drive the consumption of this tourism type, taking tourist awareness as the key element. To this awareness, we must add the current crisis experienced by the tourism industry caused by COVID-19, since it can benefit Sustainable Tourism development, promoting less crowded destinations that favor social distancing. For this, the existing literature on Sustainable Tourism has been examined in order to create a model that highlights the relations among these variables. To determine the meaning of these relations, a sample of 308 tourists was analyzed through structural equation models using Partial Least Squares. The results show that there is a clear attitude on the part of the tourist to develop Sustainable Tourism, driven by the positive effects and motivation it entails, as well as the satisfaction the tourist perceives when consuming a responsible tourism type.
Enhancing Local Disaster Management Network through Developing Resilient Community in New Taipei City, Taiwan
Authors: Ke Kai-Yuan , Lin Yong-Jun , Tan Yih-Chi , Pan Tsung-Yi , Tai Li-Li , Lee Ching-An
Published: July 2020

Large-scaled disaster events had increasingly occurred worldwide due to global and environmental change. Evidently, disaster response cannot rely merely on the public force. In the golden hour of crisis, not only the individuals should learn to react, protect themselves, and try to help each other, but also the local school, enterprise, non-government organization (NGO), nonprofit organization (NPO), and volunteer groups should collaborate to effectively deal with disaster events. New Taipei City (NTPC), Taiwan, was aware of the need for non-public force response and therefore developed the process of enhancing local disaster management networks through promoting the resilient community since 2009. The concept of a resilient community is to build community-based capacity for mitigation, preparedness, response, and recovery in an all-hazards manner. This study organized the NTPC experience and presented the standard operation procedure (SOP) to promote the resilient community, key obstacles, maintenance mechanism, and the successful formulation of the local disaster management network. The performance of the promotion was evaluated through a questionnaire survey and found that participants affirmed the positive effect of building community capacity through the entire process. In general, the resilient community as the center of the local disaster management work is shown promising to holistically bridge the inner/outer resources and systematically respond to disaster events.
Resveratrol, Curcumin and Piperine Alter Human Glyoxalase 1 in MCF-7 Breast Cancer Cells
Authors: Schmidt Betina , Ferreira Christian , Alves Passos Carlos Luan , Silva Jerson Lima , Fialho Eliane
Published: July 2020

Breast cancer is the leading cause of cancer mortality in women worldwide. Conventional cancer treatment is costly and results in many side effects. Dietary bioactive compounds may be a potential source for breast cancer prevention and treatment. In this scenario, the aim of this study was to investigate the effects of the bioactive compounds resveratrol, curcumin and piperine (R-C-P) on MCF-7 breast cancer cells and to associate them to Glyoxalase 1 (GLO1) activity. The findings indicate that R-C-P exhibits cytotoxicity towards MCF-7 cells. R-C-P decreased mitochondrial membrane potential (ΔΨm) by 1.93-, 2.04- and 1.17-fold, respectively. Glutathione and N-acetylcysteine were able to reverse the cytotoxicity of the assessed bioactive compounds in MCF-7 cells. R-C-P reduced GLO1 activity by 1.36-, 1.92- and 1.31-fold, respectively. R-C-P in the presence of antimycin A led to 1.98-, 1.65- and 2.16-fold decreases in D-lactate levels after 2 h of treatment, respectively. Glyoxal and methylglyoxal presented cytotoxic effects on MCF-7 cells, with IC50 values of 2.8 and 2.7 mM and of 1.5 and 1.4 mM after 24 and 48 h of treatment, respectively. In conclusion, this study demonstrated that R-C-P results in cytotoxic effects in MCF-7 cells and that this outcome is associated with decreasing GLO1 activity and mitochondrial dysfunction.
β-Carbolines in Experiments on Laboratory Animals
Authors: Zawirska-Wojtasiak Renata , Fedoruk-Wyszomirska Agnieszka , Piechowska Paulina , Mildner-Szkudlarz Sylwia , Bajerska Joanna , Wojtowicz Elżbieta , Przygoński Krzysztof , Gurda Dorota , Kubicka Wiktoria , Wyszko Eliza
Published: July 2020

Some studies have ascribed a protective effect against neurodegenerative diseases to the β-carbolines harman (H) and norharman (NH), which occur mostly in coffee and coffee substitutes. We determined the concentrations of β-carbolines and undesirable compounds (such as acrylamide) in roasted coffee substitute ingredients and found that chicory coffee was optimal. Two in vivo experiments were conducted with seventeen-month-old male Sprague Dawley rats fed a diet with the addition of pure carboline standards in the first stage, and chicory in the second. We observed an increase in the level of H and NH in blood plasma, as well as higher activity of animals in the battery behavioral test, particularly in the second stage. The results of in vitro studies—particularly the level of the expression in brain tissue of genes associated with aging processes and neurodegenerative diseases—clearly show the benefits of a diet rich in β-carbolines.
Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections
Authors: Lagrée Anne-Claire , Fasani Fabienne , Rouxel Clotilde , Pivet Marine , Pourcelot Marie , Fablet Aurore , Romey Aurore , Caignard Grégory , Vitour Damien , Blaise-Boisseau Sandra , Kieda Claudine , Boulouis Henri-Jean , Haddad Nadia , Grillon Catherine
Published: July 2020

Microvascular endothelial cells constitute potential targets for exogenous microorganisms, in particular for vector-borne pathogens. Their phenotypic and functional variations according to the organs they are coming from provide an explanation of the organ selectivity expressed in vivo by pathogens. In order to make available relevant tools for in vitro studies of infection mechanisms, our aim was to immortalize bovine organospecific endothelial cells but also to assess their permissivity to viral infection. Using transfection with SV40 large T antigen, six bovine microvascular endothelial cell lines from various organs and one macrovascular cell line from an umbilical cord were established. They display their own panel of endothelial progenitor/mature markers, as assessed by flow cytometry and RT-qPCR, as well as the typical angiogenesis capacity. Using both Bluetongue and foot-and-mouth disease viruses, we demonstrate that some cell lines are preferentially infected. In addition, they can be transfected and are able to express viral proteins such as BTV8-NS3. Such microvascular endothelial cell lines bring innovative tools for in vitro studies of infection by viruses or bacteria, allowing for the study of host-pathogen interaction mechanisms with the actual in vivo target cells. They are also suitable for applications linked to microvascularization, such as anti-angiogenic and anti-tumor research, growing fields in veterinary medicine.
Signaling Modulations of miR-206-3p in Tooth Morphogenesis
Authors: Neupane Sanjiv , Aryal Yam Prasad , Kim Tae-Young , Yeon Chang-Yeol , An Chang-Hyeon , Kim Ji-Youn , Yamamoto Hitoshi , Lee Youngkyun , Sohn Wern-Joo , Kim Jae-Young
Published: July 2020

MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell growth, differentiation, apoptosis, and metabolism. The miR-206-3p has been correlated with cancer; however, developmental roles of this miRNA are unclear. In this study, we examined the expression pattern and evaluated the developmental regulation of miR-206-3p during tooth morphogenesis using ex-vivo culture method. The expression pattern of miR-206-3p was examined in the epithelium and mesenchyme of developing tooth germ with stage-specific manners. Perturbation of the expression of miR-206-3p clearly altered expression patterns of dental-development–related signaling molecules, including Axin2, Bmp2, Fgf4, Lef1 and Shh. The gene expression complemented with change in cellular events including, apoptosis and proliferation which caused altered crown and pulp morphogenesis in renal-capsule–calcified teeth. Especially, mislocalization of β-Catenin and SMAD1/5/8 were observed alongside dramatic alterations in the expression patterns of Fgf4 and Shh. Overall, our data suggest that the miR-206-3p regulate the cellular physiology during tooth morphogenesis through modulation of the Wnt, Bmp, Fgf, and Shh signaling pathways to form proper tooth pulp and crown.
The Role of the Bacterial Muramyl Dipeptide in the Regulation of GLP-1 and Glycemia
Authors: Williams Laura , Alshehri Amal , Robichaud Bianca , Cudmore Alison , Gagnon Jeffrey
Published: July 2020

The host’s intestinal microbiota contributes to endocrine and metabolic responses, but a dysbiosis in this environment can lead to obesity and insulin resistance. Recent work has demonstrated a role for microbial metabolites in the regulation of gut hormones, including the metabolic hormone, glucagon-like peptide-1 (GLP-1). Muramyl dipeptide (MDP) is a bacterial cell wall component which has been shown to improve insulin sensitivity and glucose tolerance in diet-induced obese mice by acting through the nucleotide oligomerization domain 2 (NOD2) receptor. The purpose of this study was to understand the effects of MDP on GLP-1 secretion and glucose regulation. We hypothesized that MDP enhances glucose tolerance by inducing intestinal GLP-1 secretion through NOD2 activation. First, we observed a significant increase in GLP-1 secretion when murine and human L-cells were treated with a fatty acid MDP derivative (L18-MDP). Importantly, we demonstrated the expression of the NOD2 receptor in mouse intestine and in L-cells. In mice, two intraperitoneal injections of MDP (5 mg/kg body weight) caused a significant increase in fasting total GLP-1 in chow-fed mice, however this did not lead to an improvement in oral glucose tolerance. When mice were exposed to a high-fat diet, they eventually lost this MDP-induced GLP-1 release. Finally, we demonstrated in L-cells that hyperglycemic conditions reduce the mRNA expression of NOD2 and GLP-1. Together these findings suggest MDP may play a role in enhancing GLP-1 during normal glycemic conditions but loses its ability to do so in hyperglycemia.
Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts
Authors: Liu Wenqian , Xiang Chenggang , Li Xiaojun , Wang Tao , Lu Xiaohong , Liu Zixi , Gao Lihong , Zhang Wenna
Published: July 2020

Grafting has been widely used to improve plant growth and tolerance in crop production, as well as for clarifying systemic mRNA signaling from donor to recipient tissues in organ-to-organ communication. In this study, we investigated graft partner interaction mechanisms of Cucumis sativus (Csa) and Cucurbita moschata (Cmo) using a large-scale endogenous mRNA transport. The results indicated that most mobile transcripts followed an allocation pathway from source to sink. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that mRNA mobility functions are universally common and individually specific. Identification of mRNA mobility between distant tissues in heterografts with RT-PCR (reverse transcription PCR), RT-qPCR (reverse transcriptional quantitative real time PCR), and clone sequencing were used to estimate 78.75% of selected mobile transcripts. Integration of bioinformatic analysis and RT-qPCR identification allowed us to hypothesize a scion-to-rootstock-to-scion feedback signal loop of Csa move-down and Cmo move-up mRNAs, where Csa scion move-down mRNAs were involved in carbon fixation and biosynthesis of amino acid pathways, and Cmo root received Csa move-down mRNA and then delivered the corresponding Cmo upward mRNA to scion to improve photosynthesis of cucumber scion. This formed a feedback signal loop of scion-to-rootstock-to scion to explain why pumpkin rootstock enhanced cucumber production in the industry, which was utilized for organ communication and mediates photosynthesis processes in heterograft cucurbit crops.