bluuebunny/crossref_metadata_2025 huggingface dataset:
Advancements and Challenges of Ammonia as a Sustainable Fuel for the Maritime Industry
Authors: Chavando Antonio , Silva Valter , Cardoso João , Eusebio Daniela
Published: June 2024

The maritime industry needs sustainable, low-emission fuels to reduce the environmental impact. Ammonia is one of the most promising alternative fuels because it can be produced from renewable energy, such as wind and solar. Furthermore, ammonia combustion does not emit carbon. This review article covers the advantages and disadvantages of using ammonia as a sustainable marine fuel. We start by discussing the regulations and environmental concerns of the shipping sector, which is responsible for around 2% to 3% of global energy-related CO2 emissions. These emissions may increase as the maritime industry grows at a compound annual growth rate of 4.33%. Next, we analyze the use of ammonia as a fuel in detail, which presents several challenges. These challenges include the high price of ammonia compared to other fossil fuels, the low reactivity and high toxicity of ammonia, NOx, and N2O emissions resulting from incomplete combustion, an inefficient process, and NH3 slipping. However, we emphasize how to overcome these challenges. We discuss techniques to reduce NOx and N2O emissions, co-combustion to improve reactivity, waste heat recovery strategies, the regulatory framework, and safety conditions. Finally, we address the market trends and challenges of using ammonia as a sustainable marine fuel.
C-Rate- and Temperature-Dependent State-of-Charge Estimation Method for Li-Ion Batteries in Electric Vehicles
Authors: Aslan Eyyup , Yasa Yusuf
Published: June 2024

Li-ion batteries determine the lifespan of an electric vehicle. High power and energy density and extensive service time are crucial parameters in EV batteries. In terms of safe and effective usage, a precise cell model and SoC estimation algorithm are indispensable. To provide an accurate SoC estimation, a current- and temperature-dependent SoC estimation algorithm is proposed in this paper. The proposed SoC estimation algorithm and equivalent circuit model (ECM) of the cells include current and temperature effects to reflect real battery behavior and provide an accurate SoC estimation. For including current and temperature effects in the cell model, lookup tables have been used for each parameter of the model. Based on the proposed ECM, the unscented Kalman filter (UKF) approach is utilized for estimating SoC since this approach is satisfactory for nonlinear systems such as lithium-ion batteries. The experimental results reveal that the proposed approach provides superior accuracy when compared to conventional methods and it is promising in terms of meeting electric vehicle requirements.
Three-Level Secure Smart Protection for Ring Grid-Connected Distributed Generation
Authors: Bakkar Mostafa , Bogarra Santiago , Córcoles Felipe , Aboelhassan Ahmed , Wang Shuo , Iglesias Javier
Published: June 2024

The penetration increase in distributed generators (DGs) into smart grids (SGs) will lead to new challenges, especially in protection systems. In the case of ring grids, the behavior of the short-circuit current is affected by DGs, and the medium voltage (MV) transformer connections significantly influence the changes; therefore, the protection strategies must be adapted for these scenarios. This study provides a comprehensive protection system for the MV distribution system (DS), including reconfigurable smart ring grids. The proposed protection methods contain three protection algorithms. The first protection algorithm relies on communication among all protective devices (PDs) in the grid, whereas the second protection method uses communication among PDs along the same line. Then, a third algorithm built on the local data of each PD is suggested as a backup to prevent communication issues and offer more reliable protection. MATLABTM SIMULINK simulations and experimental results on a scalable hardware grid were also employed to validate the protection algorithms.
Recalibrated Correlations between Dynamic Cone Penetrometer (DCP) Data and California Bearing Ratio (CBR) in Subgrade Soil
Authors: Chokkerd Jirawat , Udomchai Artit , Sultornsanee Sivarit , Angkawisittpan Niwat , Jantosut Piyanat , Sangiamsak Noppadol , Kaewhanam Nopanom
Published: June 2024

This study investigates the correlation between the California Bearing Ratio (CBR) and the Dynamic Cone Penetrometer (DCP) for subgrade soil analysis. The paper aims to provide practical equations for predicting CBR values from DCP test results, therefore enhancing the efficiency of soil assessments in engineering practice. By analyzing test data and proposing correlations for different soil groups, the study introduces recalibrated correlations that demonstrate high accuracy in predicting CBR values. The newly proposed equations offer reliable predictions with R2 values of 0.89, 0.92, and 0.94 for clean sand, silty sand or sandy silt, and cohesive soil, respectively. These correlations serve as valuable tools for engineers, enabling rapid and accurate CBR estimations for improved decision-making in various engineering projects.
A New Algorithmic Method for Reverse Osmosis Desalination Analysis: Design Optimization and Parametric Study
Authors: Aridi Rima , Al Mawla Mohamad , Harika Elias , Lemenand Thierry , Khaled Mahmoud , Gad El-Rab Mostafa
Published: June 2024

Population growth, coupled with industrial and agricultural development, has resulted in increased demand for freshwater supply. For countries with scarce water resources, desalination constitutes the only viable solution to this problem. Reverse osmosis (RO) technology has become widely used as the membrane materials have been upgraded and the costs have been reduced. Nowadays, RO is the foremost technology for desalting different types of water such as seawater, brackish, and tap water. However, its design is critical since many parameters are involved in obtaining a good design. The high use of RO encourages the establishment of a procedure that facilitates the design process and helps in obtaining an optimum-performance RO desalination system. This paper presents a procedure divided into three parts: (1) classifying RO parameters; (2) choosing the parameters in a certain order and doing the calculation process through 12 steps; and (3) then inserting the selected parameters and the obtained values on RO System Analysis (ROSA) software. These points are then summarized by creating an algorithmic chart to follow during the design phase of the RO system using ROSA. An example on the proposed list is then taken to validate the procedure, and a comparison is conducted on choosing different values for the parameters. The results of this comparative study show that choosing different parameters affects the RO system productivity. Additionally, every design has a specific optimum set of parameters, which depends upon the design constraints set by the user.
The Issue of Estimating the Maintenance and Operation Costs of Buildings: A Case Study of a School
Authors: Obradović Dino , Briš Alić Martina , Čulo Ksenija
Published: June 2024

The operations and maintenance phase is typically the longest phase within the building life cycle. Proper and consistent building maintenance is imperative for several reasons, including extending the life of buildings, improving occupants’ comfort and safety, and ultimately saving on long-term costs. Budgets for maintenance should be foreseen. The costs of statutory periodic inspections, the costs of replacing worn materials and elements, the costs of periodic works and repairs, the costs of reactive maintenance and the costs of operation will be shown for the analyzed building—a school. This paper outlines the development of a 15-year maintenance plan and program for the building, covering the period from 2024 to 2038. The plan incorporates a discount rate of 3.64% and accounts for inflation when calculating costs. The present value of the costs of maintenance and operation of the building for 15 years is EUR 1,978,292.20 and the largest share of these costs is the operation cost of the building with EUR 1,599,002.18 (80.83%). A sensitivity analysis was conducted by varying the discount rate and analysis period, resulting in recalculated present values for maintenance costs. The analysis reveals a correlation of 26.73% between the present value of maintenance and operation costs over a 15-year period and the associated capital costs.
Pectinase Production from Cocoa Pod Husk in Submerged Fermentation and Its Application in the Clarification of Apple Juice
Authors: Rozendo Anderson Steyner , Vandenberghe Luciana Porto de Souza , de Mattos Patricia Beatriz Gruening , Rogez Hervé Louis Ghislain , Soccol Carlos Ricardo
Published: June 2024

The present work aimed to use cocoa pod husk (CPH) and its extracted pectin as a potential substrate for the production of pectinase and to test the enzyme produced in the clarification process of apple juice. CPH with a particle size of <0.84 mm was employed for pectinase production by a selected strain of Aspergillus niger NRRL 2270. The optimization of the physicochemical conditions of the production medium led to an enzymatic activity of 602.03 U/g dry CPH, which was obtained under the following conditions: 110.25 g/L of CPH, 5% w/v pectin extract, 0.05 g/L of yeast extract, incubation at 28 °C, and pH 4, representing a 176% increase in enzymatic activity under the evaluated conditions. The production kinetics of pectinase showed maximum enzymatic activity at 96 h. Subsequently, the enzymatic extract was precipitated, microfiltered, and ultrafiltrated, resulting in 4852.50 U/mg of specific activity. The enzymatic activity after recovery and purification processes corresponded to 819 U/g dry CPH. Finally, a clarification stage of apple juice was carried out, in which the produced pectinase (CauPec) showed turbidity of 448.89 NTU compared to 417.89 NTU for the commercial enzyme and a viscosity of 1.86 cP, CauPec, and 1.19 cP, commercial pectinase, as well as soluble solids of 8.0 for commercial pectinase and 8.73 for CauPec. Therefore, it can be concluded that CPH and its pectin extract were excellent substrates for the production of pectinases, whose formulation is highly stable and can be applied in the clarification of apple juice.
Development of Foam-Free Biosurfactant Production Processes Using Bacillus licheniformis
Authors: Leal Eduardo , Teixeira José A. , Gudiña Eduardo J.
Published: June 2024

Microbial biosurfactants are considered environmentally friendly alternatives to synthetic surfactants in numerous applications. One of the main bottlenecks to their widespread use is the lack of effective processes for their production on an industrial scale. Biosurfactant production using conventional aerated bioreactors results in excessive foaming due to the combination of air injection and their tensioactive properties. A not widely explored approach to overcome this problem is the development of foam-free production processes, which require the identification and characterization of appropriate microorganisms. Bacillus licheniformis EL3 was evaluated for biosurfactant production under oxygen-limiting conditions in a bioreactor, using a mineral medium containing glucose as a carbon source and NaNO3 and NH4Cl as nitrogen sources. After optimizing the operational conditions, glucose concentration, and inoculum strategy, B. licheniformis EL3 produced 75 ± 3 mg biosurfactant/L in 43 h. The purified biosurfactant exhibited exceptional surface active properties, with minimum surface tension values (29 mN/m) and a critical micelle concentration (27 mg/L) similar to those achieved with commercial surfactin. Furthermore, biosurfactant yield per substrate (YP/S = 0.007 g biosurfactant/g glucose) was similar to the figures reported for Bacillus subtilis strains grown in similar conditions, whereas biosurfactant yield per biomass (YP/X = 0.755 g biosurfactant/g biomass) and specific biosurfactant productivity (qBS = 0.018 g biosurfactant/(g biomass × h)) were almost three times higher when compared to previous reports. The results obtained indicate that B. licheniformis EL3 is a promising candidate for the development of foam-free biosurfactant production processes at an industrial scale.
Digital Identity in the EU: Promoting eIDAS Solutions Based on Biometrics
Authors: Ruiu Pietro , Saiu Salvatore , Grosso Enrico
Published: June 2024

Today, more than ever before, technological progress is evolving rapidly, and in the absence of adequate regulatory frameworks, the big players in the digital market (the so-called Big Techs) are exploiting personal data (name, address, telephone numbers) and private data (political opinions, religious beliefs, financial information, or health status) in an uncontrolled manner. A crucial role in this scenario is played by the weakness of international regulatory frameworks due to the slow response time of legislators who are incapable, from a regulatory point of view, of keeping pace with technological evolution and responding to the new requirements coming from the social context, which is increasingly characterized by the pervasive presence of new technologies, such as smartphones and wearable devices. At the European level, the General Data Protection Regulation (GDPR) and the Regulation on Electronic Identification, Authentication and Trust Services (eIDAS) have marked a significant turning point in the regulatory landscape. However, the mechanisms proposed present clear security issues, particularly in light of emerging concepts such as digital identity. Moreover, despite the centrality of biometric issues within the European regulatory framework and the practical introduction of biometric data within electronic national identity (eID) cards, there are still no efforts to use biometric features for the identification and authentication of a person in a digital context. This paper clarifies and precisely defines the potential impact of biometric-based digital identity and hypothesizes its practical use for accessing network-based services and applications commonly used in daily life. Using the Italian eID card as a model, an authentication scheme leveraging biometric data is proposed, ensuring full compliance with GDPR and eIDAS regulations. The findings suggest that such a scheme can significantly improve the security and reliability of electronic identification systems, promoting broader adoption of eIDAS solutions.
Simulation Modeling of the Process of Danger Zone Formation in Case of Fire at an Industrial Facility
Authors: Matveev Yuri , Abu-Abed Fares , Zhironkina Olga , Zhironkin Sergey
Published: June 2024

Proactive prevention and fighting fire at industrial facilities, often located in urbanized clusters, should include the use of modern methods for modeling danger zones that appear during the spread of the harmful combustion products of various chemicals. Simulation modeling is a method that allows predicting the parameters of a danger zone, taking into account a number of technological, landscape, and natural-climatic factors that have a certain variability. The purpose of this research is to develop a mathematical simulation model of the formation process of a danger zone during an emergency at an industrial facility, including an explosion of a container with chemicals and fire, with the spread of an aerosol and smoke cloud near residential areas. The subject of this study was the development of a simulation model of a danger zone of combustion gases and its graphical interpretation as a starting point for timely decision making on evacuation by an official. The mathematical model of the process of danger zone formation during an explosion and fire at an industrial facility presented in this article is based on the creation of a GSL library from data on the mass of explosion and combustion products, verification using the Wald test, and the use of algorithms for calculating the starting and ending points of the danger zone for various factor values’ variables, constructing ellipses of the boundaries of the distribution of pollution spots. The developed model makes it possible to calculate the linear dimensions and area of the danger zone under optimistic and pessimistic scenarios, constructing a graphical diagram of the zones of toxic doses from the source of explosion and combustion. The results obtained from the modeling can serve as the basis for making quick decisions about evacuating residents from nearby areas.
Physical and Thermochemical Properties of Selected Wood Species in Nigeria: A Fuel Suitability and Pelleting Potential Assessment
Authors: Yunusa Suleiman Usman , Narra Satyanarayana , Mensah Ebenezer , Preko Kwasi , Saleh Aminu
Published: June 2024

Scientific studies on the impact of wood species on solid fuel production, performance, and sustainability are grossly inadequate. The knowledge of this is imperative as users of solid fuels are increasing rapidly, especially in Africa. On this note, it becomes necessary to explore measures that will improve its efficiency and sustainability as an energy source. This study investigates some properties of selected wood species used as an energy source in Nigeria and their pelleting potential. Nine samples were characterized and assessed for suitability of pelleting following four wood pellet quality standards. The properties investigated are physical (moisture content and density) and thermochemical (calorific value, ash content, volatile matter, fixed carbon, and ultimate properties (carbon, nitrogen, hydrogen, oxygen, sulfur, arsenic, cadmium, and lead)). These were selected because they are among the most important pellet parameters contained in the quality standards. The findings revealed a net calorific value between 10.61 MJ.kg−1 for Tectona grandis and 18.44 MJ.kg−1 for Eucalyptus cam. The ash content, volatile matter, and fixed carbon contents of the samples range between 2.1 and 24.4%, 65.94 and 87.77%, and 3.51 and 18.63%, respectively. Anogeissus leiocarpus was found to be the species with the best rating score in terms of fuel properties, while Vitellaria paradoxa was the lowest. However, in terms of conformity with the four wood pellet standards, Khaya senegalensis, Parkia biglobosa, and Eucalyptus cam., having presented density, calorific value, sulfur, arsenic, cadmium, and lead within the limits of the wood pellet quality standards, were considered the best wood species in terms of fuel suitability and pelleting potential. The findings therefore suggest that not all wood species are suitable as fuel. Thus, for species that do not meet the standard wood pellet requirements, alternatives such as the use of biomass blends, additives, or process adjustments can be employed to adapt the quality to the standards or by using the fuels in improved cookstoves.
Oral Hygiene Profile of Schoolchildren from Bucharest, Romania—How It Can Be Used and Improved for Better Prevention of Oral Diseases
Authors: Funieru Cristian , Nicolescu Mihnea Ioan , Băicuş Cristian , Slușanschi Oana , Bica Clara Ilinca , Moldoveanu Andreea , Perpelea Anca Cristina , Țandără Adrian Teodor
Published: June 2024

Background: Oral hygiene is essential for low caries or gingivitis prevalence in children. This study aimed to determine the oral hygiene profile of children from secondary schools (10–17 years) in Bucharest, Romania, and to explore options for preventing their future oral diseases. Methods: The study was cross-sectional, with a sample of n = 1595 pupils. The sample was stratified by clusters in city areas, grades, and the criterion of the presence/absence of in-school dental service. Dental check-ups were performed by using a commune dental examination kit. The oral hygiene simplified index (OHI; Silness and Löe) was used to assess the children’s oral hygiene status. Five questions were used to determine oral hygiene habits. Results: The mean value of the OHI was 1.04. Some differences in oral hygiene scores were found for different socioeconomic variables, such as parents’ education (p < 0.05): parents with vs. without a university degree—0.95/0.94 (male/female) vs. 1.06. Most of the children used manual toothbrushes (88%). Conclusions: The analysis of objective data (OHI) revealed a good level of oral hygiene, with many socioeconomic disparities.
A Rare Onset of T-Lymphoid Blast Crisis in Chronic Myeloid Leukemia with Two Distinct Blast Populations
Authors: Mongia Alessandra , Romano Francesca , Ciullini Mannurita Sara , Peruzzi Benedetta , Bencini Sara , Parrini Daniela , Fasano Laura , Fanelli Alessandra
Published: June 2024

Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by bone marrow expansion and the proliferation of one or more myeloid cell lineages, predominantly driven by the expression of the constitutively active fusion product tyrosine kinase BCR:ABL1. Rarely, CML patients directly develop a blast crisis (BC), mostly of myeloid origin. CML at blast crisis with a T-cell phenotype at diagnosis, without any prior history of CML, is extremely rare. Herein, we describe one rare CML case, in a young man showing an unusual and early T-lymphoid blastic crisis at diagnosis, as the first onset of a previously unknown CML. The multidisciplinary collaboration between laboratorians and clinicians for the diagnosis and management of this atypical case was crucial in outlining both a targeted pharmacological treatment and a successful hematopoietic stem cell transplantation.
Towards a Capability Maturity Model for Micro-Credential Providers in European Higher Education
Authors: Iatrellis Omiros , Samaras Nicholas , Kokkinos Konstantinos
Published: June 2024

Navigating the complexities of establishing sustainable micro-credentialing programs in European higher education requires a tailored approach for providers. The goal of this research is to develop an assessment tool that enhances the implementation and recognition of micro-credentials in European higher education. This article introduces the INVEST Micro-credentialing Capability Maturity Model (MCMM), designed to assist educational institutions in assessing and enhancing their readiness for micro-credential establishment. Through a systematic six-stage process, we identify supportive conditions, specify levels of process maturity, develop domains and aspects, characterize levels of capability, consult with stakeholders, and finalize the model. The resulting Capability Maturity Model (CMM) outlines five levels of process maturity encompassing educational, administrative, regulatory, and collaborative aspects, aligning with the principles outlined by the European Union (EU) initiatives for micro-credential establishment. Through establishing clear objectives, the model aids in the strategic development and evaluation of micro-credential programs. Stakeholders are offered a comprehensive roadmap to evaluate their progress, identify required resources, and strategize for further advancement in micro-credentialing initiatives.
Analysis of Landscape Pattern Evolution and Impact Factors in the Mainstream Basin of the Tarim River from 1980 to 2020
Authors: Jiang Lili , Li Yating
Published: June 2024

The mainstream basin of the Tarim River serves as a vital ecological security barrier that prevents the merging and expansion of deserts and an important strategic corridor directly linking Qinghai and Xinjiang. With society’s development and climate change, ecological issues such as river interruption, vegetation degradation, and land desertification in the basin have notably intensified, and the ecological security is facing a critical test. Exploring the characteristics of landscape changes and their driving factors within the basin is crucial in improving the ecological environment system’s management. Based on land use data from 1980 to 2020, this study analyzed the characteristics of the spatiotemporal changes and pattern evolution of the landscape through a landscape transfer matrix and landscape pattern indices. It further revealed the impact factors of the landscape pattern through canonical correspondence analysis. The results showed that (1) in 1980–2020, the areas of desert, forest, farmland, and settlement landscapes increased, while the area of grassland landscape decreased, and the water landscape showed an “increasing–decreasing–recovery” pattern. The landscape transition types mainly included the transition from grassland to desert; mutual transitions among farmland, grassland, and forests; mutual transitions between water and grassland; and the transition from farmland to settlements. (2) The overall landscape pattern demonstrated increased fragmentation, shape complexity, and evenness with decreased aggregation. Furthermore, different landscapes exhibited distinct characteristics of landscape pattern changes; for instance, grassland landscape showed severe fragmentation, while desert landscape displayed the strongest dominance. (3) The landscape pattern was a result of the combined impact of natural and human factors, with the soil thickness (SOT), road density (ROD), annual actual evapotranspiration (AAE), population density (POD), and mean annual temperature (MAT) exhibiting significant influences. Specifically, the settlement and farmland landscapes were mainly influenced by the mean annual relative humidity (MAH), POD, GDP density (GDP), and distance to artificial water (DAW); the forest, grassland, and water landscapes were mainly influenced by the SOT, soil organic matter content (SOM), AAE, ROD, elevation (ELE), MAT, slope (SLP), and distance to natural water (DNW); and the desert landscape was mainly influenced by the DAW, DNW, SLP, AAE, SOT, SOM, and ROD. These findings can provide a scientific reference for landscape management and restoration, as well as sustainable social and economic development, in the mainstream basin of the Tarim River.
Alcohol Consumption Norms and the Favored Alcohol Consumption Policies of Citizens of Seoul
Authors: Kang Jina , Han Hyeri , Kim Hyeongsu , Park AhHyun , Kim Dasom , Hwang Rahil , Kim Miyoung , Ko Young , Jung Sungwon
Published: June 2024

The purpose of this study was to define the alcohol consumption norms and attitudes toward alcohol regulation policies among citizens of Seoul and the relationships between such norms and the favored regulatory policies. The study population consisted of 1001 adults aged 19–80 years living in Seoul. We collected demographic data and data on health behaviors, attitudes towards drinking, and preferred alcohol regulation policies. The correlations between drinking and the favored regulatory policies were analyzed. Male, as well as being employed, aged 19–39 years, single, a smoker, and a current or heavy episodic drinker were associated with more positive attitudes toward drinking (all p < 0.001) and less desire for alcohol regulation policies (all p < 0.001). We found a significant negative correlation between attitudes toward drinking and preferred alcohol regulation policies (p < 0.001). Participants who favored reduced or no alcohol consumption and a reduction in alcohol-related harm were more accepting of restrictive alcohol consumption policies. To establish alcohol control polices, differences in drinking norms within populations should be considered. Furthermore, for a successful alcohol control policy, efforts should be made to change drinking norms, as well as consider differences in regulatory policy preferences between population groups.
A Synergistic Strategy Combining Chemotherapy and Photodynamic Therapy to Eradicate Prostate Cancer
Authors: Shirke Aditi A. , Walker Ethan , Chavali Sriprada , Ramamurthy Gopalakrishnan , Zhang Lifang , Panigrahi Abhiram , Basilion James P. , Wang Xinning
Published: June 2024

Prostate cancer is the most prevalent cancer among men in the United States and is a leading cause of cancer-related death. Prostate specific membrane antigen (PSMA) has been established as a biomarker for prostate cancer diagnosis and treatment. This study aimed to develop a novel theranostic agent, PSMA-1-MMAE-Pc413, which integrates a PSMA-targeting ligand, the photosensitizer Pc413, and the microtubular inhibitor monomethyl auristatin E (MMAE) for synergistic therapeutic efficacy. In vitro uptake studies revealed that PSMA-1-MMAE-Pc413 demonstrated selective and specific uptake in PSMA-positive PC3pip cells but not in PSMA-negative PC3flu cells, with the uptake in PC3pip cells being approximately three times higher. In vitro cytotoxicity assays showed that, when exposed to light, PSMA-1-MMAE-Pc413 had a synergistic effect, leading to significantly greater cytotoxicity in PSMA-positive cells (IC50 = 2.2 nM) compared to PSMA-1-Pc413 with light irradiation (IC50 = 164.9 nM) or PSMA-1-MMAE-Pc413 without light irradiation (IC50 = 12.6 nM). In vivo imaging studies further demonstrated the selective uptake of PSMA-1-MMAE-Pc413 in PC3pip tumors. In in vivo studies, PSMA-1-MMAE-Pc413 dramatically improves the therapeutic outcome for prostate cancer by providing a synergistic effect that surpasses the efficacy of each treatment modality alone in PC3pip tumors. These findings suggest that PSMA-1-MMAE-Pc413 has strong potential for clinical application in improving prostate cancer treatment.
Preoperative Immune Cell Dysregulation Accompanies Ovarian Cancer Patients into the Postoperative Period
Authors: Ulevicius Jonas , Jasukaitiene Aldona , Bartkeviciene Arenida , Dambrauskas Zilvinas , Gulbinas Antanas , Urboniene Daiva , Paskauskas Saulius
Published: June 2024

Ovarian cancer (OC) poses a significant global health challenge with high mortality rates, emphasizing the need for improved treatment strategies. The immune system’s role in OC progression and treatment response is increasingly recognized, particularly regarding peripheral blood mononuclear cells (PBMCs) and cytokine production. This study aimed to investigate PBMC subpopulations (T and B lymphocytes, natural killer cells, monocytes) and cytokine production, specifically interleukin-1 beta (IL-1β), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-12 (IL-12), and tumor necrosis factor alpha (TNFα), in monocytes of OC patients both preoperatively and during the early postoperative period. Thirteen OC patients and 23 controls were enrolled. Preoperatively, OC patients exhibited changes in PBMC subpopulations, including decreased cytotoxic T cells, increased M2 monocytes, and the disbalance of monocyte cytokine production. These alterations persisted after surgery with subtle additional changes observed in PBMC subpopulations and cytokine expression in monocytes. Considering the pivotal role of these altered cells and cytokines in OC progression, our findings suggest that OC patients experience an enhanced pro-tumorigenic environment, which persists into the early postoperative period. These findings highlight the impact of surgery on the complex interaction between the immune system and OC progression. Further investigation is needed to clarify the underlying mechanisms during this early postoperative period, which may hold potential for interventions aimed at improving OC management.
Human Acellular Collagen Matrices—Clinical Opportunities in Tissue Replacement
Authors: Verboket René D. , Henrich Dirk , Janko Maren , Sommer Katharina , Neijhoft Jonas , Söhling Nicolas , Weber Birte , Frank Johannes , Marzi Ingo , Nau Christoph
Published: June 2024

The field of regenerative medicine is increasingly in need of effective and biocompatible materials for tissue engineering. Human acellular dermal matrix (hADM)-derived collagen matrices stand out as a particularly promising candidate. Their ability to preserve structural integrity, coupled with exceptional biocompatibility, positions them as a viable choice for tissue replacement. However, their clinical application has been largely confined to serving as scaffolds. This study aims to expand the horizon of clinical uses for collagen sheets by exploring the diverse cutting-edge clinical demands. This review illustrates the clinical utilizations of collagen sheets beyond traditional roles, such as covering skin defects or acting solely as scaffolds. In particular, the potential of Epiflex®, a commercially available and immediately clinically usable allogeneic membrane, will be evaluated. Collagen sheets have demonstrated efficacy in bone reconstruction, where they can substitute the induced Masquelet membrane in a single-stage procedure, proving to be clinically effective and safe. The application of these membranes allow the reconstruction of substantial tissue defects, without requiring extensive plastic reconstructive surgery. Additionally, they are found to be apt for addressing osteochondritis dissecans lesions and for ligament reconstruction in the carpus. The compelling clinical examples showcased in this study affirm that the applications of human ADM extend significantly beyond its initial use for skin defect treatments. hADM has proven to be highly successful and well-tolerated in managing various etiologies of bone and soft tissue defects, enhancing patient care outcomes. In particular, the application from the shelf reduces the need for additional surgery or donor site defects.
Enhancing Efficiency of Nitrate Reduction to Ammonia by Fe and Co Nanoparticle-Based Bimetallic Electrocatalyst
Authors: Kuznetsova Irina , Lebedeva Olga , Kultin Dmitry , Mashkin Mikhail , Kalmykov Konstantin , Kustov Leonid
Published: June 2024

The green and sustainable electrocatalytic conversion of nitrogen-containing compounds to ammonia is currently in high demand in order to replace the eco-unfriendly Haber–Bosch process. Model catalysts for the nitrate reduction reaction were obtained by electrodeposition of metal Co, Fe, and bimetallic Fe/Co nanoparticles from aqueous solutions onto a graphite substrate. The samples were characterized by the following methods: SEM, XRD, XPS, UV–vis spectroscopy, cyclic (and linear) voltammetry, chronoamperometry, and electrochemical impedance spectroscopy. In addition, the determination of the electrochemically active surface was also performed for all electrocatalysts. The best electrocatalyst was a sample containing Fe-nanoparticles on the layer of Co-nanoparticles, which showed a Faradaic efficiency of 58.2% (E = −0.785 V vs. RHE) at an ammonia yield rate of 14.6 μmol h−1 cm−2 (at ambient condition). An opinion was expressed to elucidate the mechanism of coordinated electrocatalytic action of a bimetallic electrocatalyst. This work can serve primarily as a starting point for future investigations on electrocatalytic conversion reactions to ammonia using model catalysts of the proposed type.
Prediction of the Aggressive Clinical Course of Papillary Thyroid Carcinoma Based on Fine Needle Aspiration Biopsy Molecular Testing
Authors: Lukyanov Sergei A. , Titov Sergei E. , Kozorezova Evgeniya S. , Demenkov Pavel S. , Veryaskina Yulia A. , Korotovskii Denis V. , Ilyina Tatyana E. , Vorobyev Sergey L. , Zhivotov Vladimir A. , Bondarev Nikita S. , Sleptsov Ilya V. , Sergiyko Sergei V.
Published: June 2024

Molecular genetic events are among the numerous factors affecting the clinical course of papillary thyroid carcinoma (PTC). Recent studies have demonstrated that aberrant expression of miRNA, as well as different thyroid-related genes, correlate with the aggressive clinical course of PTC and unfavorable treatment outcomes, which opens up new avenues for using them in the personalization of the treatment strategy for patients with PTC. In the present work, our goal was to assess the applicability of molecular markers in the preoperative diagnosis of aggressive variants of papillary thyroid cancer. The molecular genetic profile (expression levels of 34 different markers and BRAF mutations) was studied for 108 cytology specimens collected by fine-needle aspiration biopsy in patients with PTC having different clinical manifestations. Statistically significant differences with adjustment for multiple comparisons (p < 0.0015) for clinically aggressive variants of PTC were obtained for four markers: miRNA-146b, miRNA-221, fibronectin 1 (FN1), and cyclin-dependent kinase inhibitor 2A (CDKN2A) genes. A weak statistical correlation (0.0015 < p < 0.05) was observed for miRNA-31, -375, -551b, -148b, -125b, mtDNA, CITED1, TPO, HMGA2, CLU, NIS, SERPINA1, TFF3, and TMPRSS4. The recurrence risk of papillary thyroid carcinoma can be preoperatively predicted using miRNA-221, FN1, and CDKN2A genes.
Comment on Lai et al. Dipeptidyl Peptidase 4 Stimulation Induces Adipogenesis-Related Gene Expression of Adipose Stromal Cells. Int. J. Mol. Sci. 2023, 24, 16101
Authors: Cordero Oscar J. , Kotrulev Martin , Gomez-Touriño Iria
Published: June 2024

Adiponectin is a circulating hormone secreted by adipose tissue that exerts, unlike other adipokines such as leptin, anti-inflammatory, anti-atherosclerotic and other protective effects on health. Adiponectin receptor agonists are being tested in clinical trials and are expected to show benefits in many diseases. In a recent article, LW Chen’s group used monocyte chemoattractant protein-1 (MCP-1/CCL2) to improve plasma levels of adiponectin, suggesting the involvement of dipeptidyl peptidase 4 (DPP4/CD26) in the mechanism. Here, we discuss the significance of the role of DPP4, favoring the increase in DPP4-positive interstitial progenitor cells, a finding that fits with the greater stemness and persistence of other DPP4/CD26-positive cells.
Deciphering Immune Responses to Immunization via Transcriptional Analysis: A Narrative Review of the Current Evidence towards Personalized Vaccination Strategies
Authors: Papadatou Ioanna , Geropeppa Maria , Piperi Christina , Spoulou Vana , Adamopoulos Christos , Papavassiliou Athanasios G.
Published: June 2024

The development of vaccines has drastically reduced the mortality and morbidity of several diseases. Despite the great success of vaccines, the immunological processes involved in protective immunity are not fully understood and several issues remain to be elucidated. Recently, the advent of high-throughput technologies has enabled a more in-depth investigation of the immune system as a whole and the characterization of the interactions of numerous components of immunity. In the field of vaccinology, these tools allow for the exploration of the molecular mechanisms by which vaccines can induce protective immune responses. In this review, we aim to describe current data on transcriptional responses to vaccination, focusing on similarities and differences of vaccine-induced transcriptional responses among vaccines mostly in healthy adults, but also in high-risk populations, such as the elderly and children. Moreover, the identification of potential predictive biomarkers of vaccine immunogenicity, the effect of age on transcriptional response and future perspectives for the utilization of transcriptomics in the field of vaccinology will be discussed.
Reducing FKBP51 Expression in the Ventral Hippocampus Decreases Auditory Fear Conditioning in Male Rats
Authors: Irizarry-Méndez Nashaly , Criado-Marrero Marangelie , Hernandez Anixa , Colón Maria , Porter James T.
Published: June 2024

Fear conditioning evokes a physiologic release of glucocorticoids that assists learning. As a cochaperone in the glucocorticoid receptor complex, FKBP51 modulates stress-induced glucocorticoid signaling and may influence conditioned fear responses. This study combines molecular and behavioral approaches to examine whether locally reducing FKBP51 expression in the ventral hippocampus is sufficient to affect fear-related behaviors. We hypothesized that reducing FKBP51 expression in the VH would increase glucocorticoid signaling to alter auditory fear conditioning. Adult male rats were injected with an adeno-associated virus (AAV) vector expressing short hairpin – RNAs (shRNA) targeting FKBP5 into the ventral hippocampus to reduce FKBP5 levels or a control AAV. Infusion of FKBP5-shRNA into the ventral hippocampus decreased auditory fear acquisition and recall. Although animals injected with FKBP5-shRNA showed less freezing during extinction recall, the difference was due to a reduced fear recall rather than improved extinction. Reducing ventral hippocampus FKBP51 did not affect exploratory behavior in either the open field test or the elevated zero maze test but did increase passive behavior in the forced swim test, suggesting that the reduction in auditory fear recall was not due to more active responses to acute stress. Furthermore, lower ventral hippocampus FKBP51 levels did not alter corticosterone release in response to restraint stress, suggesting that the reduced fear recall was not due to lower corticosterone release. Our findings suggest FKBP51 in the ventral hippocampus plays a selective role in modulating fear-learning processes and passive behavioral responses to acute stress rather than hypothalamic-pituitary-adrenal axis reactivity or exploratory responses.
Nanostructure-Mediated Transport of Therapeutics through Epithelial Barriers
Authors: Hansen M. Eva , Ibrahim Yasmin , Desai Tejal A. , Koval Michael
Published: June 2024

The ability to precisely treat human disease is facilitated by the sophisticated design of pharmacologic agents. Nanotechnology has emerged as a valuable approach to creating vehicles that can specifically target organ systems, effectively traverse epithelial barriers, and protect agents from premature degradation. In this review, we discuss the molecular basis for epithelial barrier function, focusing on tight junctions, and describe different pathways that drugs can use to cross barrier-forming tissue, including the paracellular route and transcytosis. Unique features of drug delivery applied to different organ systems are addressed: transdermal, ocular, pulmonary, and oral delivery. We also discuss how design elements of different nanoscale systems, such as composition and nanostructured architecture, can be used to specifically enhance transepithelial delivery. The ability to tailor nanoscale drug delivery vehicles to leverage epithelial barrier biology is an emerging theme in the pursuit of facilitating the efficacious delivery of pharmacologic agents.
Extracellular Vesicles Derived from Opuntia ficus-indica Fruit (OFI-EVs) Speed Up the Normal Wound Healing Processes by Modulating Cellular Responses
Authors: Valentino Anna , Conte Raffaele , Bousta Dalila , Bekkari Hicham , Di Salle Anna , Calarco Anna , Peluso Gianfranco
Published: June 2024

Plant-derived extracellular vesicles (EVs) have been recognized as important mediators of intercellular communication able to transfer active biomolecules across the plant and animal kingdoms. EVs have demonstrated an impressive array of biological activities, displaying preventive and therapeutic potential in mitigating various pathological processes. Indeed, the simplicity of delivering exogenous and endogenous bioactive molecules to mammalian cells with their low cytotoxicity makes EVs suitable agents for new therapeutic strategies for a variety of pathologies. In this study, EVs were isolated from Opuntia ficus-indica fruit (OFI-EVs) and characterized by particle size distribution, concentration, and bioactive molecule composition. OFI-EVs had no obvious toxicity and demonstrated a protective role in the inflammatory process and oxidative stress in vitro model of chronic skin wounds. The results demonstrated that pretreatment with OFI-EVs decreased the activity and gene expression of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) in the LPS-stimulated human leukemia monocytic cell line (THP-1). Furthermore, OFI-EVs promote the migration of human dermal fibroblasts (HDFs), speeding up the normal wound healing processes. This study sheds light, for the first time, on the role of OFI-EVs in modulating important biological processes such as inflammation and oxidation, thereby identifying EVs as potential candidates for healing chronic cutaneous wounds.
Molecular Mechanisms of Dementia 2.0
Authors: Cantone Mariagiovanna
Published: June 2024

Dementia and the other neurodegenerative disorders represent a complex pathophysiological process [...]
Astaxanthin Induces Apoptosis in MCF-7 Cells through a p53-Dependent Pathway
Authors: Kim Koanhoi , Cho Hyok-rae , Son Yonghae
Published: June 2024

Astaxanthin (3,3′-dihydroxy-β,β-carotene-4,4′-dione; AXT) is a xanthophyll β-carotenoid found in microalgae, seafood, fungi, complex plants, flamingos, and quail. It is well known that AXT plays a role as a drug with antioxidant and antitumor properties. Furthermore, several studies have reported that the reagent shows anti-inflammatory and neuroprotective effects. Recently, it was found that AXT acts as a peroxisome proliferator-activated receptor γ (PPARγ) modulator. To investigate the effect of AXT on MCF-7 cells (a human breast cancer cell line), the cells were treated with various concentrations of AXT. The treatment induced the decrease in cell number in a dose-dependent manner. Additionally, the Annexin V-positive cells were increased by the AXT treatment. These results indicated that apoptosis was induced in the tumor cells through the treatment of AXT. To elucidate the connection between apoptosis and p53, the levels of p53 and p21 proteins were assessed. Consequently, it was observed that the expression of p53 and p21 increased proportionally to the concentration of the AXT treatment. These findings suggest that the apoptosis of MCF-7 cells induced by AXT operates through a p53-dependent pathway, implying that AXT could potentially have a beneficial role in future breast cancer treatments. Thus, our results will provide a direction for future cancer challenges.
Anti-Persisters Activity of Lacticaseibacillus rhamnosus Culture Filtrates against Pseudomonas aeruginosa in Artificial Sputum Medium
Authors: Bianchi Marta , Esin Semih , Kaya Esingül , Batoni Giovanna , Maisetta Giuseppantonio
Published: June 2024

Persisters are antibiotic-tolerant bacteria, playing a role in the recalcitrance and relapse of many bacterial infections, including P. aeruginosa pulmonary infections in Cystic Fibrosis (CF) patients. Among novel antimicrobial strategies, the use of probiotics and their products is emerging as a particularly promising approach. The aim of this study was to evaluate the anti-persisters activity of culture filtrate supernatants of Lacticaseibacillus rhamnosus (LRM-CFS) against P. aeruginosa in artificial sputum medium (ASM), which resembles the CF lung environment. Planktonic persisters of two clinical strains of P. aeruginosa (PaCF1 and PaCF4) were obtained following two different procedures: (i) exposing stationary-phase cultures to cyanide m-chlorophenylhydrazone (CCCP) in LB medium; (ii) incubating stationary-phase cultures with high doses of tobramycin (128-fold MIC) in ASM. In addition, persisters from biofilm were obtained by exposing 48 h old biofilm of P. aeruginosa to 128 x MIC of ciprofloxacin. LRM-CFS at dilutions of 1:6 and 1:4 resulted in being bactericidal in ASM against both PaCF1 and PaCF4 persisters obtained after CCCP or tobramycin treatment. Moreover, LRM-CFS at dilution 1:4 caused a reduction of antibiotic-tolerant bacteria in the biofilm of both P. aeruginosa strains. Overall, LRM-CFS represents a promising adjuvant therapeutic strategy against P. aeruginosa recalcitrant infections in CF patients.