bluuebunny/crossref_metadata_2025 huggingface dataset:
Ascorbylation of a Reactive Cysteine in the Major Apple Allergen Mal d 1
Authors: Ahammer Linda , Unterhauser Jana , Eidelpes Reiner , Meisenbichler Christina , Nothegger Bettina , Covaciu Claudia E. , Cova Valentina , Kamenik Anna S. , Liedl Klaus R. , Breuker Kathrin , Eisendle Klaus , Reider Norbert , Letschka Thomas , Tollinger Martin
Published: September 2022

The protein Mal d 1 is responsible for most allergic reactions to apples (Malus domestica) in the northern hemisphere. Mal d 1 contains a cysteine residue on its surface, with its reactive side chain thiol exposed to the surrounding food matrix. We show that, in vitro, this cysteine residue is prone to spontaneous chemical modification by ascorbic acid (vitamin C). Using NMR spectroscopy and mass spectrometry, we characterize the chemical structure of the cysteine adduct and provide a three-dimensional structural model of the modified apple allergen. The S-ascorbylated cysteine partially masks a major IgE antibody binding site on the surface of Mal d 1, which attenuates IgE binding in sera of apple-allergic patients. Our results illustrate, from a structural perspective, the role that chemical modifications of allergens with components of the natural food matrix can play.
Sirtfoods: New Concept Foods, Functions, and Mechanisms
Authors: Akan Otobong Donald , Qin Dandan , Guo Tianyi , Lin Qinlu , Luo Feijun
Published: September 2022

Sirtfood is a new concept food that compounds diets that can target sirtuins (SIRTs). SIRTs are nicotinamide adenine dinucleotide (NAD+)-dependent deacylases and ADP-ribosyltransferases (enzymes). SIRTs are mediators of calorie restriction (CR) and their activation can achieve some effects similar to CR. SIRTs play essential roles in ameliorating obesity and age-related metabolic diseases. Food ingredients such as resveratrol, piceatannol, anthocyanidin, and quinine are potential modulators of SIRTs. SIRT modulators are involved in autophagy, apoptosis, aging, inflammation, and energy homeostasis. Sirtfood proponents believe that natural Sirtfood recipes exert significant health effects.
The Changes in Bell Pepper Flesh as a Result of Lacto-Fermentation Evaluated Using Image Features and Machine Learning
Authors: Ropelewska Ewa , Sabanci Kadir , Aslan Muhammet Fatih
Published: September 2022

Food processing allows for maintaining the quality of perishable products and extending their shelf life. Nondestructive procedures combining image analysis and machine learning can be used to control the quality of processed foods. This study was aimed at developing an innovative approach to distinguishing fresh and lacto-fermented red bell pepper samples involving selected image textures and machine learning algorithms. Before processing, the pieces of fresh pepper and samples subjected to spontaneous lacto-fermentation were imaged using a digital camera. The texture parameters were extracted from images converted to different color channels L, a, b, R, G, B, X, Y, and Z. The textures after selection were used to build models for the classification of fresh and lacto-fermented samples using algorithms from the groups of Lazy, Functions, Trees, Bayes, Meta, and Rules. The highest average accuracy of classification reached 99% for the models developed based on sets of selected textures for color space Lab using the IBk (instance-based K-nearest learner) algorithm from the group of Lazy, color space RGB using SMO (sequential minimal optimization) from Functions, and color space XYZ and color channel X using IBk (Lazy) and SMO (Functions). The results confirmed the differences in image features of fresh and lacto-fermented red bell pepper and revealed the effectiveness of models built based on textures using machine learning algorithms for the evaluation of the changes in the pepper flesh structure caused by processing.
Two Polysaccharides from Liupao Tea Exert Beneficial Effects in Simulated Digestion and Fermentation Model In Vitro
Authors: Qiu Siqi , Huang Li , Xia Ning , Teng Jianwen , Wei Baoyao , Lin Xiaoshan , Khan Muhammad Rafiullah
Published: September 2022

Liupao tea is an important dark tea, but few studies on purified Liupao tea polysaccharide (TPS) are reported in the literature. In this study, two TPSs, named TPS2 and TPS5, with molecular weights of 70.5 and 133.9 kDa, respectively, were purified from Liupao tea. TPS2 contained total sugar content (53.73% ± 1.55%) and uronic acid content (35.18% ± 0.96%), while TPS5 was made up of total sugar (51.71% ± 1.1%), uronic acid (40.95% ± 3.12%), polyphenols (0.43% ± 0.03%), and proteins (0.11% ± 0.07%). TPS2 and TPS5 were composed of Man, Rha, GlcA, Glc, Gal, and Ara in the molar ratios of 0.12:0.69:0.20:0.088:1.60:0.37 and 0.090:0.36:0.42:0.07:1.10:0.16, respectively. The effects of TPS2 and TPS5 on digestion and regulation of gut microbiota in hyperlipidemic rats were compared. In simulated digestion, TPS5 was degraded and had good antioxidant effect, whereas TPS2 was not affected. The bile acids binding capacities of TPS2 and TPS5 were 42.79% ± 1.56% and 33.78% ± 0.45%, respectively. During in vitro fermentation, TPS2 could more effectively reduce pH, promote the production of acetic acid and propionic acid, and reduce the ratio of Firmicutes to Bacteroidetes. TPS5 could more effectively promote the production of butyric acid and increase the abundance of genus Bacteroides. Results indicate that polysaccharides without polyphenols and proteins have better antidigestibility and bile acid binding. Meanwhile, polysaccharides with polyphenols and proteins have a better antioxidant property. Both have different effects on the gut microbiota.
“Key Factor” for Baijiu Quality: Research Progress on Acid Substances in Baijiu
Authors: Wu Yashuai , Hou Yaxin , Chen Hao , Wang Junshan , Zhang Chunsheng , Zhao Zhigang , Ao Ran , Huang He , Hong Jiaxin , Zhao Dongrui , Sun Baoguo
Published: September 2022

Baijiu is the national liquor of China, which has lasted in China for more than 2000 years. Abundant raw materials, multi-strain co-fermentation, and complex processes make the secrets of baijiu flavor and taste still not fully explored. Acid substances not only have a great influence on the flavor and taste of baijiu, but also have certain functions. Therefore, this paper provides a systematic review for the reported acid substances, especially for their contribution to the flavor and functional quality of baijiu. Based on previous studies, this paper puts forward a conjecture, a suggestion, and a point of view, namely: the conjecture of “whether acid substances can be used as ‘key factor’ for baijiu quality “; the suggestion of “the focus of research on acid substances in baijiu should be transferred to evaluating their contribution to the taste of baijiu”; and the view of “acid substances are ‘regulators’ in the fermentation process of baijiu”. It is worth thinking about whether acid substances can be used as the key factors of baijiu to be studied and confirmed by practice in the future. It is hoped that the systematic review of acid substances in baijiu in this paper can contribute to further in-depth and systematic research on baijiu by researchers in the future.
Stable Isotope Analysis for the Discrimination of the Geographical Origin of Greek Bottarga ‘Avgotaracho Messolongiou’: A Preliminary Research
Authors: Thomatou Anna-Akrivi , Psarra Eleni , Mazarakioti Eleni C. , Katerinopoulou Katerina , Tsirogiannis Georgios , Zotos Anastasios , Kontogeorgos Achilleas , Patakas Angelos , Ladavos Athanasios
Published: September 2022

Consumers are increasingly interested in the geographical origin of the foodstuff they consume as an important characteristic of food authenticity and quality. To assure the authenticity of the geographical origin, various methods have been proposed. Stable isotope analysis is a method that has been extensively used for products such as wine, oil, meat, while only a few studies have been conducted for the discrimination of seafood origin and especially for mullet roes or bottarga products. Analysis of the stable isotopes of C, N and S of Bottarga samples from four different origins were carried out. The values of δ15N (5.45‰) and δ34S (4.66‰) for the Greek Bottarga Product named ‘Avgotaracho Messolongiou’, from Messolongi lagoon were lower than other areas while δ13C values were higher (−14.84‰). The first results show that the stable isotopes ratios of carbon, nitrogen and sulphur could be used to discriminate the Greek Protected Designations of Origin Bottarga product ‘Avgotaracho Messolongiou’ from other similar products.
Efficiency of Regional Airports: Insights on the Effect of Airline Type and Seasonal Variations in Traffic
Authors: Pagoni Ioanna , Koumoutsidi Annitsa
Published: September 2022

This paper aims at exploring the efficiency of regional airports, whose traffic is highly dependent on touristic flows, and the impact of some external factors such as low-cost airlines, charter air traffic and seasonality. The analysis focuses on the airport market in Greece within the time period from 2010 to 2016. A bootstrapped data envelopment analysis model is developed and the Malmquist Productivity Index is computed to estimate the total productivity change between 2010 and 2016. This is followed by a tobit regression model to estimate the impact of the external factors on the airport efficiency scores. Our findings indicate that the considered factors significantly affect airport efficiency. More specifically, the low-cost airlines and charter flights contribute to increasing airport efficiency, while the seasonality might be seen as an obstacle to improving airport efficiency. To the best of our knowledge, this research goes beyond any previous study in the Greek airport market and could be useful for several practitioners, such as airlines, airport operators and hotel businesses, as well as policy-makers and authorities.
Selective Penetration and Profile Control Performance of Preformed Particle Gels for Heterogeneous Oil Reservoirs
Authors: Zhou Kang , Wu Dejun , An Zhibin , Liu Shuai
Published: September 2022

The preformed particle gel (PPG) has been proved to be an effective chemical agent to reduce fluid channeling and increase the sweeping efficiency. However, we still lack a clear understanding of the field-scale matching relationship between PPG size, elastic modulus and a heterogeneous reservoir. In this respect, the paper carried out various sand pack displacement experiments. The results indicated that an excessively large PPG or elastic modulus would plug a low-permeability sand pack and even increase the severity of fluid channeling. On the contrary, an excessively small PPG or elastic modulus allowed a certain degree of profile control, but the PPG could easily migrate out of high-permeability sand packs with water. If the elastic modulus remained unchanged, the suitable PPG size increased as the reservoir permeability ratio increased. On the other hand, the suitable elastic modulus increased with the increase of the reservoir permeability ratio when the PPG size was kept the same. By using regression analysis, quantitative expressions were established in order to determine the best suitable PPG size for a certain heterogeneous reservoir. When the elastic modulus was fixed, the best suitable PPG mesh exhibited a linear relation with the permeability ratio. This paper provides a useful reference to select the most convenient PPG size and elastic modulus for a potential heterogeneous reservoir, suitable to enhance oil recovery.
Hydrogel Improved Growth and Productive Performance of Mango Trees under Semi-Arid Condition
Authors: Alshallash Khalid S. , Sharaf Mohamed , Hmdy Ashraf E. , Khalifa Sobhy M. , Abdel-Aziz Hosny F. , Sharaf Ahmed , Ibrahim Mariam T. S. , Alharbi Khadiga , Elkelish Amr
Published: September 2022

Nowadays, the production of new mango cultivars is increased in many countries worldwide. The soil application of hydrogel represents a novel approach in the fruit trees industry. This investigation aims to study the effect of adding hydrogel (as soil conditioner) on the growth and yield of Shelly cv. mango trees. The experimental groups were assigned to a control group and three other treated groups, including 250, 500, or 750 g hydrogel∙tree−1. The results demonstrated that all applications of hydrogel composite had higher vegetative growth parameters, yield, and fruit quality characteristics of Shelly cv. mango trees compared to the control. The treatment of 750 g hydrogel∙tree−1 had higher values of vegetative growth parameters such as the leaf area, shoot length and tree canopy volume, compared to the control group and the other treatments. Similarly, higher values for yield and fruit quality were observed in the treatment of 750 g hydrogel∙tree−1. In conclusion, different amounts of hydrogel agent can improve the production and fruit quality of Shelly cv. mango trees in arid and semi-arid conditions in a dose-dependent manner.
Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering
Authors: Ma Yuan , Wang Xinhui , Su Ting , Lu Feng , Chang Qiang , Gao Jianhua
Published: September 2022

Hydrogels have been extensively used as scaffolds in tissue engineering for cell adhesion, proliferation, migration, and differentiation because of their high-water content and biocompatibility similarity to the extracellular matrix. However, submicron or nanosized pore networks within hydrogels severely limit cell survival and tissue regeneration. In recent years, the application of macroporous hydrogels in tissue engineering has received considerable attention. The macroporous structure not only facilitates nutrient transportation and metabolite discharge but also provides more space for cell behavior and tissue formation. Several strategies for creating and functionalizing macroporous hydrogels have been reported. This review began with an overview of the advantages and challenges of macroporous hydrogels in the regulation of cellular behavior. In addition, advanced methods for the preparation of macroporous hydrogels to modulate cellular behavior were discussed. Finally, future research in related fields was discussed.
Analysis of Building Height Impact on Land Surface Temperature by Digital Building Height Model Obtained from AW3D30 and SRTM
Authors: Danniswari Dibyanti , Honjo Tsuyoshi , Furuya Katsunori
Published: September 2022

Land surface temperature (LST) is heavily influenced by urban morphology. Building height is an important parameter of urban morphology that affects LST. Existing studies show contradicting results where building height can have a positive or negative relationship with LST. More studies are necessary to examine the impact of building height. However, high accuracy building height data are difficult to obtain on a global scale and are not available in many places in the world. Using the Digital Building Height Model (DBHM) calculated by subtracting the SRTM from AW3D30, this study analyzes the relationship between building height and Landsat LST in two cities: Tokyo and Jakarta. The relationship is observed during both cities’ warm seasons (April to October) and Tokyo’s cool seasons (November to February). The results show that building height and LST are negatively correlated. In the morning, areas with high-rise buildings tend to have lower LST than areas with low-rise buildings. This phenomenon is revealed to be stronger during the warm season. The LST difference between low-rise and mixed-height building areas is more significant than between mixed-height and high-rise building areas.
How Physical Activity Affects Knee Cartilage and a Standard Intervention Procedure for an Exercise Program: A Systematic Review
Authors: Petrigna Luca , Roggio Federico , Trovato Bruno , Zanghì Marta , Guglielmino Claudia , Musumeci Giuseppe
Published: September 2022

(1) Background: Cartilage degeneration with the natural aging process and the role of physical activity on cartilage wellness is still not clear. The objective of the present review was to understand how different physical activity interventions affect the cartilage and to propose a Standard Operating Procedure for an exercise program to maintain knee joint health; (2) Methods: Articles were collected on three different electronic databases and screened against the eligibility criteria. Results were collected in tables and the main outcomes were discussed narratively; (3) Results: A total of 24 studies have been included after the screening process and aerobic, strength, flexibility, postural balance, and mobility interventions were detected. Different protocols and types of interventions were adopted by the authors; (4) Conclusions: Physical activity interventions have mainly positive outcomes on cartilage structure, but the protocols adopted are different and various. A Standard Operating Procedure has been proposed for a physical intervention focalized on cartilage wellness that could be adopted as an intervention in the clinical setting. Furthermore, the creation of a standardized protocol wants to help scientific research to move in the same direction.
Development and Validation of a Questionnaire to Assess Knowledge and Attitudes toward COVID-19 Preventive Measures in Romania
Authors: Popa Alina Delia , Antoniu Sabina Antonela , Enache Armand Iustinian , Popa Iolanda Valentina , Dragomir Raluca Alina , Burlacu Alexandru
Published: September 2022

The World Health Organization warns about the threat of the COVID-19 sixth wave. Our aim was to propose the first validated Romanian questionnaire to assess people’s level of education and attitudes towards general measures to protect against COVID-19 infection. Our study was conducted on a sample of 194 people. The first version of the questionnaire consisted of 40 items. Items that did not meet psychometric criteria were removed. Latent components/factors were identified through exploratory factorial analysis (EFA). The Cronbach’s alpha coefficient was used to assess internal fidelity. The EFA identified three factors. Factor 1 was named “Compliance with protective measures”, factor 2 was “Attitudes toward vaccination” and factor 3 was “Attitudes regarding potential COVID-19 therapies”. The final version of the questionnaire consists of 16 items. The test’s final score predicted the presence of vaccination with an accuracy of 0.773. The questionnaire score, the diagnosis of diabetes, the advice provided by healthcare workers and the medical profession proved to be significant predictors of vaccination. The implementation of our questionnaire within national programs could identify populational areas that need specific interventions to reach vaccination targets and prevent a full-blown sixth wave of the COVID-19 pandemic in Romania.
Factor Analysis of Quality Management Systems Implementation in Healthcare: An Online Survey
Authors: Rawshdeh Mustafa , Keathley Heather , Obeidat Shahed , Athamenh Raed , Tanash Moayad , Bani Hani Dania
Published: September 2022

This paper investigates the views of healthcare researchers and professionals on the implementation of the Quality Management System (QMS) approach using a 5-point Likert scale survey. Researchers and healthcare professionals who observed or participated in QMS implementation were surveyed. Multiple channels, including occupational societies, social networking, i.e., LinkedIn, hospital’s directories, award recipients, academic researchers, and professional connections, made it possible to reach this particular sample. Participants were surveyed using a series of questions with a total of 56 questions. The survey was administrated through the web portal of Qualtrics and then analyzed both on Qualtrics and SPSS software packages. Descriptive Statistics, Exploratory Factor Analysis (EFA), and Linear Regression were employed to draw conclusions. The final sample group consisted of 71 participants representing a range of healthcare settings. EFA was conducted, producing a model of 10 emergent factors and an outcome for total improvement. Regression modeling revealed the Critical Success Factors (CSFs) and the interaction between emergent factors. The results indicated that QMS Implementation Culture, Structure, and Managerial Training are critical to the QMS implementation success. This research helps quality professionals enhance their ability to prioritize elements affecting the successful implementation of the QMS.
Implementing the STEADY Wellness Program to Support Healthcare Workers throughout the COVID-19 Pandemic
Authors: Korman Melissa B. , Steinberg Rosalie , Gagliardi Lina , Stewart Brenda , Acero Carmen Llanos , Davies Joanne , Maunder Robert , Walker Thomas , DasGupta Tracey , DiProspero Lisa , Sinyor Mark , Ellis Janet
Published: September 2022

The COVID-19 pandemic has posed an ongoing threat to the mental wellbeing of countless individuals worldwide, with healthcare workers at particularly high risk. We developed the STEADY staff wellness program prior to the pandemic based on the available literature and input from stakeholders, guided by the Knowledge-to-Action (KTA) Implementation Science Framework. We quickly adapted the STEADY program for implementation in selected high-need units within Canada’s largest trauma hospital during the pandemic’s first wave. This brief report describes implementation of the STEADY program, retroactively applying the structure of the Knowledge-to-Action Implementation Science Framework to the practical steps taken. We identified the importance of more frequent, shorter contact with HCWs that occurred in-person, with an emphasis on peer support. A flexible approach with strong support from hospital leadership were key facilitators. Our findings suggest that a flexible approach to practical program implementation, theoretically underpinned in best-practices, can result in an acceptable program that promotes increased HCW wellbeing during a pandemic.
Classification Algorithms Used in Predicting Glaucoma Progression
Authors: Tarcoveanu Filip , Leon Florin , Curteanu Silvia , Chiselita Dorin , Bogdanici Camelia Margareta , Anton Nicoleta
Published: September 2022

In this paper, various machine learning algorithms were used in order to predict the evolution of open-angle glaucoma (POAG). The datasets were built containing clinical observations and objective measurements made at the Countess of Chester Hospital in the UK and at the “St. Spiridon” Hospital of Iași, Romania. Using these datasets, different classification problems were proposed. The evaluation of glaucoma progression was conducted based on parameters such as VFI (Visual field index), MD (Mean Deviation), PSD (Pattern standard deviation), and RNFL (Retinal Nerve Fiber Layer). As classification tools, the following algorithms were used: Multilayer Perceptron, Random Forest, Random Tree, C4.5, k-Nearest Neighbors, Support Vector Machine, and Non-Nested Generalized Exemplars. The best results, with an accuracy of over 90%, were obtained with Multilayer Perceptron and Random Forest algorithms. The NNGE algorithm also proved very useful in creating a hierarchy of the input values according to their influence (weight) on the considered outputs. On the other hand, the decision tree algorithms gave us insight into the logic used in their classification, which is of practical importance in obtaining additional information regarding the rationale behind a certain rule or decision.
Parental Optimism Improves Youth Psychological Well-Being: Family Cohesion and Youth Optimism as Serial Mediators
Authors: Qi Wei , Shi Jing , Cui Lijuan
Published: September 2022

Focusing on the family system, this study simultaneously examined the effects of the parental factor, family functioning, and individual factor on youth psychological well-being. Overall, 332 youths and their parents were involved in this research and responded to an online questionnaire measuring parental optimism, family cohesion, youth optimism, and youth psychological well-being. The results suggested that (1) parental optimism was positively related to youth psychological well-being; (2) both family cohesion and youth optimism mediated the connection between parental optimism and youth psychological well-being; and (3) the link between parental optimism and youth psychological well-being was mediated by family cohesion and youth optimism in sequence. The present study reveals the underlying mechanism of how to improve youth psychological well-being from within the family system.
Pharmacist Intervention in Portuguese Older Adult Care
Authors: Rodrigues Ana Rita , Teixeira-Lemos Edite , Mascarenhas-Melo Filipa , Lemos Luís Pedro , Bell Victoria
Published: September 2022

Healthy ageing has become one of the most significant challenges in a society with an increasing life expectancy. Older adults have a greater prevalence of chronic disease, with the need for multiple medications to appropriately control these issues. In addition to their health concerns, ageing individuals are prone to loneliness, dependence, and economic issues, which may affect their quality of life. Governments and health professionals worldwide have developed various strategies to promote active and healthy ageing to improve the quality of life of older adults. Pharmacists are highly qualified health professionals, easily accessible to the population, thus playing a pivotal role in medication management. Their proximity to the patient puts them in a unique position to provide education and training to improve therapeutic adherence and identify medication-related problems. This paper aims to address the importance of Portuguese community pharmacists in the medication management of older adults, emphasising their intervention in health promotion, patient education, medication-related problems, deprescription, dose administration aids, and medication review and reconciliation. We also discuss home delivery services and medication management in long-term care facilities.
Effects of Different Rootstocks and Storage Temperatures on Postharvest Quality of Eggplant (Solanum melongena L. cv. Madonna)
Authors: Kappel Noémi , Mozafarian Maryam
Published: September 2022

In addition to mitigating biotic and abiotic stress, grafting may influence the fruit quality and postharvest quality of eggplants. Few studies have been published on the influence of grafting on the postharvest performance of eggplant fruit. The current work examined the postharvest behavior of grafted and non-grafted eggplant cv. Madonna at 0 and 10 °C storage. Rootstocks include Solanum grandiflorum × Solanum melongena (SH), Solanum torvum (ST), Solanum melongena × Solanum integrifolium (SI), Solanum integrifolium (A), and Solanum lycopersicum cv. Optifort (O) and Emperador (E). The values for soluble solids, L*, and b* of pulp declined throughout storage. The pH of the fruit pulp decreased during storage at 10 °C. The lowest firmness was observed in fruit grafted onto E and O. The oxidation potential (OP) value decreased for fruit harvested from the O rootstock. At 0 °C, the oxidation potential (OP) value increased in fruit harvested from the A rootstock. Fruit firmness reduction at the end of storage in fruit grafted onto SH was less than in the other rootstocks and control plants. Overall, we found that the storage temperature had a more significant effect than the applied rootstock on the studied parameters of the eggplant fruits.
First Hydrogenation of TiFe with Addition of 20 wt.% Ti
Authors: Ulate-Kolitsky Elena , Tougas Bernard , Huot Jacques
Published: September 2022

In this paper, we report the first hydrogenation (activation) of a 1.2Ti-0.8Fe alloy synthesized by induction melting (9 kg ingot). The alloy presented a three-phase structure composed of a main TiFe phase, a secondary Ti2Fe phase and a Ti-rich BCC phase. The alloy required cold rolling to achieve activation at room temperature. However, it did so with good kinetics, reaching saturation (2.6 wt.% H) in about 6 h. After activation, the phases identified were TiFe, Ti2FeHx and an FCC phase. The Ti2FeHx and FCC are the stable hydrides formed by the secondary Ti2Fe and BCC phases, respectively. The stoichiometry of the Ti2FeHx was calculated to be between x = 3.2–4.75. As the microstructure obtained by an industrial-scale synthesis method (induction melting) may be different than the one obtained by laboratory-scale method (arc melting), a small 3 g sample of Ti1.2Fe0.8 was synthesized by arc melting. The lab-scale sample activated (2 wt.% H in ~12 h) without the need for cold rolling. The phases identified for the lab-scale sample matched those found for the induction-melted sample. The phase fractions differed between the samples; the lab-scale sample presented a lower abundance and a finer distribution of the secondary phases. This explains the difference in the kinetics and H capacity. Based on these results it can be concluded that the alloy of composition, 1.2Ti-0.8Fe, can absorb hydrogen without the need for a heat treatment, and that finer microstructures have a strong influence on the activation kinetics regardless of the secondary phases’ phase fractions.
Busy Boards, Entrenched Directors and Corporate Innovation
Authors: Bolton Brian , Zhao Jing
Published: September 2022

We provide a comprehensive study of how different corporate governance mechanisms influence corporate innovation. Using panel data regression analysis across a sample of more than 13,600 firm-years for firms based in the United States between 1996–2010, we find that entrenched boards, though commonly associated with lower firm value, actually generate substantial innovation. We find that busy boards hinder innovation unless they also have interlocking relationships. Conversely, interlocked directors enhance innovation, unless they are busy. Directors who are CEOs or Board Chairs at other companies hinder innovation. Interestingly, despite being significant determinants of firm value in other studies, director experience, independence and ownership are not related to innovation. In order to be innovative, firms should appoint directors to leverage their professional relationships and directors must have a long-term perspective.
Comparative Study of M(Ⅱ)Al (M=Co, Ni) Layered Double Hydroxides for Silicone Foam: Characterization, Flame Retardancy, and Smoke Suppression
Authors: Zhou Lin-Lin , Li Wen-Xiong , Zhao Hai-Bo , Zhao Bin
Published: September 2022

To compare the different actions of the two representative transition metal cations of Co2+ and Ni2+ in layered double hydroxides (LDHs), CoAl-LDH and NiAl-LDH intercalated with CO32− were synthesized, and the chemical structures, microstructures, and surface areas thereof were successfully characterized. Then, the two LDHs were utilized as flame retardants and smoke suppressants for silicone foam (SiF). The densities, flame retardancy, smoke suppression, thermal stabilities, and compressive strengths of the two SiF/LDHs nanocomposites were investigated. The introduction of LDHs slightly decreased the density of SiF due to the catalytic actions of Co and Ni during the foaming process of SiF. With respect to the flame retardancy, the addition of only 1 phr of either CoAl-LDH or NiAl-LDH could effectively improve the limiting oxygen index of SiF from 28.7 to 29.6%. Based on the results of vertical flame testing and a cone calorimeter test, the flame retardancy and fire safety of the SiF were effectively enhanced by the incorporation of LDHs. In addition, owing to the good catalytic action and large specific surface area (NiAl-LDH: 174.57 m2 g−1; CoAl-LDH: 51.47 m2 g−1), NiAl-LDH revealed higher efficiencies of flame retardancy and smoke suppression than those of CoAl-LDH. According to the results of energy-dispersive X-ray spectroscopy, Co and Ni participated in the formation of protective char layers, which inhibited the release of SiO2 into the gas phase. Finally, the influences on the thermal decomposition and compressive strength for SiF resulting from the addition of LDHs are discussed.
Variant-Specific Analysis Reveals a Novel Long-Range RNA-RNA Interaction in SARS-CoV-2 Orf1a
Authors: Dukeshire Matthew , Schaeper David , Venkatesan Pravina , Manzourolajdad Amirhossein
Published: September 2022

Since the start of the COVID-19 pandemic, understanding the pathology of the SARS-CoV-2 RNA virus and its life cycle has been the priority of many researchers. Currently, new variants of the virus have emerged with various levels of pathogenicity and abundance within the human-host population. Although much of viral pathogenicity is attributed to the viral Spike protein’s binding affinity to human lung cells’ ACE2 receptor, comprehensive knowledge on the distinctive features of viral variants that might affect their life cycle and pathogenicity is yet to be attained. Recent in vivo studies into the RNA structure of the SARS-CoV-2 genome have revealed certain long-range RNA-RNA interactions. Using in silico predictions and a large population of SARS-CoV-2 sequences, we observed variant-specific evolutionary changes for certain long-range RRIs. We also found statistical evidence for the existence of one of the thermodynamic-based RRI predictions, namely Comp1, in the Beta variant sequences. A similar test that disregarded sequence variant information did not, however, lead to significant results. When performing population-based analyses, aggregate tests may fail to identify novel interactions due to variant-specific changes. Variant-specific analyses can result in de novo RRI identification.
Elevated Cerebrospinal Fluid Proteins and Albumin Determine a Poor Prognosis for Spinal Amyotrophic Lateral Sclerosis
Authors: Assialioui Abdelilah , Domínguez Raúl , Ferrer Isidro , Andrés-Benito Pol , Povedano Mónica
Published: September 2022

Amyotrophic lateral sclerosis (ALS) is a heterogeneous disease, both in its onset phenotype and in its rate of progression. The aim of this study was to establish whether the dysfunction of the blood–brain barrier (BBB) and blood–spinal cord barrier (BSCB) measured through cerebrospinal fluid (CSF) proteins and the albumin-quotient (QAlb) are related to the speed of disease progression. An amount of 246 patients diagnosed with ALS were included. CSF and serum samples were determined biochemically for different parameters. Survival analysis based on phenotype shows higher probability of death for bulbar phenotype compared to spinal phenotype (p-value: 0.0006). For the effect of CSF proteins, data shows an increased risk of death for spinal ALS patients as the value of CSF proteins increases. The same model replicated for CSF albumin yielded similar results. Statistical models determined that the lowest cut-off value for CSF proteins able to differentiate patients with a good prognosis and worse prognosis corresponds to CSF proteins ≥ 0.5 g/L (p-value: 0.0189). For the CSF albumin, the QAlb ≥0.65 is associated with elevated probability of death (p-value: 0.0073). High levels of QAlb are a bad prognostic indicator for the spinal phenotype, in addition to high CSF proteins levels that also act as a marker of poor prognosis.
Preparation of Multicolour Solid Fluorescent Carbon Dots for Light-Emitting Diodes Using Phenylethylamine as a Co-Carbonization Agent
Authors: An Yulong , Liu Can , Li Yan , Chen Menglin , Zheng Yunwu , Tian Hao , Shi Rui , He Xiahong , Lin Xu
Published: September 2022

Carbon dots (CDs), as a new type of photoluminescent nanomaterial, have attracted extensive attention in various fields because of their unique luminescence properties. However, CDs will exhibit fluorescence quenching in the solid state or aggregate state, which limits their application. In this paper, a unique strategy is proposed to regulate solutions to achieve multicolour fluorescence of CDs in the solid state. We report the successful preparation of orange, green and blue solid fluorescent CDs using citric acid, urea and phenylethylamine as precursors and methanol, ethanol and water as solvents, respectively. The solid-state fluorescence of CDs may be caused by the linkage of the phenylethyl structure to the surface of CDs during formation, which effectively disperses the CDs and prevents π–π interactions between graphitized nuclei. Meanwhile, multicolour solid fluorescent CDs are realized by adjusting the solvent in the preparation process. Based on the excellent fluorescence properties of CDs, orange, green and blue light-emitting diodes (LEDs) are prepared. A white LED (WLED) can be obtained by mixing the three colours of solid fluorescent CDs, which shows the application potential of CDs in display lighting equipment.
Alterations of Central Liver Metabolism of Pediatric Patients with Non-Alcoholic Fatty Liver Disease
Authors: Berndt Nikolaus , Hudert Christian A. , Eckstein Johannes , Loddenkemper Christoph , Henning Stephan , Bufler Philip , Meierhofer David , Sack Ingolf , Wiegand Susanna , Wallach Iwona , Holzhütter Hermann-Georg
Published: September 2022

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in children and is associated with overweight and insulin resistance (IR). Almost nothing is known about in vivo alterations of liver metabolism in NAFLD, especially in the early stages of non-alcoholic steatohepatitis (NASH). Here, we used a complex mathematical model of liver metabolism to quantify the central hepatic metabolic functions of 71 children with biopsy-proven NAFLD. For each patient, a personalized model variant was generated based on enzyme abundances determined by mass spectroscopy. Our analysis revealed statistically significant alterations in the hepatic carbohydrate, lipid, and ammonia metabolism, which increased with the degree of obesity and severity of NAFLD. Histologic features of NASH and IR displayed opposing associations with changes in carbohydrate and lipid metabolism but synergistically decreased urea synthesis in favor of the increased release of glutamine, a driver of liver fibrosis. Taken together, our study reveals already significant alterations in the NASH liver of pediatric patients, which, however, are differently modulated by the simultaneous presence of IR.
Keratocyte Differentiation Is Regulated by NF-κB and TGFβ Signaling Crosstalk
Authors: Zhou Xin , Li Junhong , Backman Ludvig J. , Danielson Patrik
Published: September 2022

Interleukin-1 (IL-1) and transforming growth factor-beta (TGFβ) are important cytokines involved in corneal wound healing. Here, we studied the effect of these cytokines on corneal stromal cell (keratocyte) differentiation. IL-1β treatment resulted in reduced keratocyte phenotype, as evident by morphological changes and decreased expression of keratocyte markers, including keratocan, lumican, ALDH3A1, and CD34. TGFβ1 treatment induced keratocyte differentiation towards the myofibroblast phenotype. This was inhibited by simultaneous treatment with IL-1β, as seen by inhibition of α-SMA expression, morphological changes, and reduced contractibility. We found that the mechanism of crosstalk between IL-1β and TGFβ1 occurred via regulation of the NF-κB signaling pathway, since the IL-1β induced inhibition of TGFβ1 stimulated keratocyte-myofibroblast differentiation was abolished by a specific NF-κB inhibitor, TPCA-1. We further found that Smad7 participated in the downstream signaling. Smad7 expression level was negatively regulated by IL-1β and positively regulated by TGFβ1. TPCA-1 treatment led to an overall upregulation of Smad7 at mRNA and protein level, suggesting that NF-κB signaling downregulates Smad7 expression levels in keratocytes. All in all, we propose that regulation of cell differentiation from keratocyte to fibroblast, and eventually myofibroblast, is closely related to the opposing effects of IL-1β and TGFβ1, and that the mechanism of this is governed by the crosstalk of NF-κB signaling.
Poly(Butylene Adipate/Terephthalate-Co-Glycolate) Copolyester Synthesis Based on Methyl Glycolate with Improved Barrier Properties: From Synthesis to Structure-Property
Authors: Wang Yanning , Yang Haicun , Li Bingjian , Liu Shi , He Mingyang , Chen Qun , Li Jinchun
Published: September 2022

The main problem of manufacturing with traditional biodegradable plastics is that it is more expensive than manufacturing with polymers derived from petroleum, and the application scope is currently limited due to poor comprehensive performance. In this study, a novel biodegradable poly(butylene adipic acid/terephthalate-co-glycolic acid) (PBATGA) copolyester with 25–60% glycolic acid units was successfully synthesized by esterification and polycondensation using cheap coal chemical byproduct methyl glycolate instead of expensive glycolic acid. The structure of the copolyester was characterized by ATR-FTIR, 1H NMR, DSC, and XRD; and its barrier property, water contact angle, heat resistance, and mechanical properties were tested. According to the experiment result, the PBATGA copolyesters showed improved oxygen (O2) and water vapor barrier character, and better hydrophilicity when compared with PBAT. The crystallization peaks of PBATGAs were elevated from 64 °C to 77 °C when the content of the GA unit was 25 mol %, meanwhile, the elongation at the break of PBATGA25 was more than 1300%. These results indicate that PBATGA copolyesters have good potentiality in high O2 and water vapor barrier and degradable packaging material.
Senescence of Tumor Cells in Anticancer Therapy—Beneficial and Detrimental Effects
Authors: Piskorz Wiktoria Monika , Cechowska-Pasko Marzanna
Published: September 2022

Cellular senescence process results in stable cell cycle arrest, which prevents cell proliferation. It can be induced by a variety of stimuli including metabolic stress, DNA damage, telomeres shortening, and oncogenes activation. Senescence is generally considered as a process of tumor suppression, both by preventing cancer cells proliferation and inhibiting cancer progression. It can also be a key effector mechanism for many types of anticancer therapies such as chemotherapy and radiotherapy, both directly and through bioactive molecules released by senescent cells that can stimulate an immune response. Senescence is characterized by a senescence-associated secretory phenotype (SASP) that can have both beneficial and detrimental impact on cancer progression. Despite the negatives, attempts are still being made to use senescence to fight cancer, especially when it comes to senolytics. There is a possibility that a combination of prosenescence therapy—which targets tumor cells and causes their senescence—with senotherapy—which targets senescent cells, can be promising in cancer treatment. This review provides information on cellular senescence, its connection with carcinogenesis and therapeutic possibilities linked to this process.
The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium–Legume Symbiosis
Authors: Jiménez-Guerrero Irene , Medina Carlos , Vinardell José María , Ollero Francisco Javier , López-Baena Francisco Javier
Published: September 2022

Rhizobia are soil bacteria that can establish a symbiotic association with legumes. As a result, plant nodules are formed on the roots of the host plants where rhizobia differentiate to bacteroids capable of fixing atmospheric nitrogen into ammonia. This ammonia is transferred to the plant in exchange of a carbon source and an appropriate environment for bacterial survival. This process is subjected to a tight regulation with several checkpoints to allow the progression of the infection or its restriction. The type 3 secretion system (T3SS) is a secretory system that injects proteins, called effectors (T3E), directly into the cytoplasm of the host cell, altering host pathways or suppressing host defense responses. This secretion system is not present in all rhizobia but its role in symbiosis is crucial for some symbiotic associations, showing two possible faces as Dr. Jekyll and Mr. Hyde: it can be completely necessary for the formation of nodules, or it can block nodulation in different legume species/cultivars. In this review, we compile all the information currently available about the effects of different rhizobial effectors on plant symbiotic phenotypes. These phenotypes are diverse and highlight the importance of the T3SS in certain rhizobium–legume symbioses.
Rhamnolipid Micellization and Adsorption Properties
Authors: Zhang Yi , Placek Tess L. , Jahan Ruksana , Alexandridis Paschalis , Tsianou Marina
Published: September 2022

Biosurfactants are naturally occurring amphiphiles that are being actively pursued as alternatives to synthetic surfactants in cleaning, personal care, and cosmetic products. On the basis of their ability to mobilize and disperse hydrocarbons, biosurfactants are also involved in the bioremediation of oil spills. Rhamnolipids are low molecular weight glycolipid biosurfactants that consist of a mono- or di-rhamnose head group and a hydrocarbon fatty acid chain. We examine here the micellization of purified mono-rhamnolipids and di-rhamnolipids in aqueous solutions and their adsorption on model solid surfaces. Rhamnolipid micellization in water is endothermic; the CMC (critical micellization concentration) of di-rhamnolipid is lower than that of mono-rhamnolipid, and both CMCs decrease upon NaCl addition. Rhamnolipid adsorption on gold surface is mostly reversible and the adsorbed layer is rigid. A better understanding of biosurfactant self-assembly and adsorption properties is important for their utilization in consumer products and environmental applications.
Walnut Shell Biowaste Valorization via HTC Process for the Removal of Some Emerging Pharmaceutical Pollutants from Aqueous Solutions
Authors: Țurcanu Anca Andreea , Matei Ecaterina , Râpă Maria , Predescu Andra Mihaela , Berbecaru Andrei-Constantin , Coman George , Predescu Cristian
Published: September 2022

This research emphasizes the performance of some eco-friendly carbon materials as hydrochars (HC) obtained by the hydrothermal carbonization (HTC) process applied to walnut shell (WS) biowaste. These materials display promising properties that can be used for environmental applications such as emerging pharmaceutical pollutant retention from water sources. Thus, three hydrochars coded HCWS1, HCWS2, and HCWS3 were obtained using a dynamic autoclave in specific conditions—temperature of 220 °C, autogenous pressure, 1:10 biomass–water weight ratio—and for three different reaction times, 1 h, 6 h, and 12 h. The HCWSs were characterized by means of ATR-FTIR and SEM-EDS analyses and tested as possible adsorbents to assess the removal efficiencies of some emerging pharmaceutical pollutants (paracetamol and methylene blue) by UV–VIS spectrophotometry. Kinetic and adsorption studies were carried out. The best results were obtained for the HCWS3 hydrochar. Further perspectives include an activation step of the hydrochars and their testing on other emerging pharmaceutical pollutants.
Vitamin D and Weight Change: A Mendelian Randomization, Prospective Study
Authors: Patriota Pollyanna , Rezzi Serge , Guessous Idris , Marques-Vidal Pedro
Published: September 2022

The association between 25-hydroxyvitamin D and 5-, 10-, or 15-year weight change were assessed in a population-based, prospective study conducted in Lausanne, Switzerland. Data from the first (2009–2012, N = 3527, 51.3% women), second (2014–2017, N = 3237, 53.8% women), and third (2018–2021, N = 2567, 54.2% women) follow-ups were used. A weighted genetic risk score (GRS) of 115 SNPs associated with vitamin D levels was constructed. At baseline, the GRS correlated positively with 25-hydroxyvitamin D levels based on a Spearman rank correlation and 95% confidence interval: 0.198 (0.166; 0.231), p < 0.001; and with body mass index: 0.036 (0.004; 0.068), p = 0.028. No association was found between quartiles of GRS and weight changes at 5, 10, or 15 years: multivariate-adjusted weight changes ± SEM at 5-years follow-up were 1.39 ± 0.17, 1.13 ± 0.17, 1.24 ± 0.17, and 1.00 ± 0.17 kg for the first to the fourth quartile of the GRS, respectively (p = 0.401). Two-step linear regression showed a significant but clinically meaningless association between GRS-derived vitamin D and weight change at 5- and 15-years: slope and 95% confidence interval for a 5 nmol/L increase in GRS-derived 25-hydroxyvitamin D levels: 0.082 (0.013; 0.150) and 0.130 (0.018; 0.243) kg, respectively. We conclude that there is little association between genetically determined 25-hydroxyvitamin D levels and weight gain.