bluuebunny/crossref_metadata_2025 huggingface dataset:
Time-course effects of exercise intervention on executive function in adolescents with obesity
Authors: Hu Lingling , Shen Qiqi , Yin Hengchan , Cui Lei
Published: September 2024

Objective

This study was to investigate the developmental characteristics of executive function (EF) in obese adolescents and the time-course effects of a 14-week exercise intervention combining aerobic exercise and resistance training on EF in this population.

Methods

The experimental group of 28 obese junior high school students participated in the exercise intervention combining aerobic exercise and resistance training, while the control group of 24 healthy weight junior high school students engaged in the regular recess exercise. EF, including inhibition, working memory, and cognitive flexibility, was assessed 1 week prior to the exercise intervention and at 12 and 14 weeks post-intervention. Changes in EF sub-functions in both groups at different time points during the exercise intervention were analyzed.

Results

The findings revealed that obese junior high school students exhibited lower levels of inhibition (p = 0.003, Cohen’s d = 0.848) and cognitive flexibility (p = 0.013, Cohen’s d = 0.706) compared to their healthy weight peers. The exercise intervention combining aerobic exercise and resistance training led to significant improvements in EF among obese junior high school students, with inhibition (p < 0.01, Cohen’s d = 0.713; p = 0.003, Cohen’s d = 0.683) and cognitive flexibility (p = 0.001, Cohen’s d = 0.797; p < 0.01, Cohen’s d = 0.890) showing significant improvement at 12 and 14 weeks post-intervention, and working memory demonstrating significant improvement at 14 weeks (p = 0.004, Cohen’s d = 0.710). No significant differences were observed in EF over time in healthy weight junior high school students.

Conclusion

Obese adolescents had impaired EF, as evidenced by low levels of the inhibition and cognitive flexibility compared to healthy weight adolescents. The exercise intervention combining aerobic exercise and resistance training had a positive effect on EF of obese adolescents. The time-course effects of the intervention on improvements in inhibition, working memory, and cognitive flexibility varied with intervention duration in obese adolescents, with significant changes in inhibition and cognitive flexibility observed at 12 weeks and significant changes in working memory at 14 weeks.
Strengthening the military stoic tradition: enhancing resilience in military service members and public safety personnel through functional disconnection and reconnection
Authors: McElheran Megan , Annis Franklin C. , Duffy Hanna A. , Chomistek Tessa
Published: September 2024

This paper addresses operational stress injuries (OSIs) among military service members (SM) and public safety personnel (PSP) resulting from prolonged exposure to potentially psychologically traumatic events (PPTEs). While psychotherapeutic interventions for post-traumatic stress injuries (PTSIs) are well established, there is a significant gap in evidence-based mental health training programs addressing proactive mitigation of negative outcomes from PPTEs. Building on the Functional Disconnection/Functional Reconnection (FD/FR) model, we introduce FD/FR 2, emphasizing early identification and management of psychological risks. FD/FR 2 discusses the practice of emotional suppression, or “pseudo-stoicism,” and its potential negative impact on mental health. By integrating authentic Stoic principles, FD/FR 2 offers practical exercises to enhance resilience and well-being, addressing a critical need in current training approaches for military SM and PSP.
Illness cognitions and parental stress symptoms following a child’s cancer diagnosis
Authors: Lietaviete Inese , Martinsone Baiba
Published: September 2024

Objective

This research aims to explore parents’ cognitive beliefs, specifically illness cognitions, in response to their children being diagnosed with cancer. This study is an initial step toward providing regular psychosocial assessment as a standard for psychosocial care for children with cancer and their families in Latvia.

Methods

Data were collected from 120 parents (mostly mothers, n = 109) as an initial evaluation of the psychosocial risks faced by families participating in the support program “Holistic and Multidisciplinary Support for Children with Functional Disabilities and Their Family Members,” which was managed by the Children’s Hospital Foundation at the Children’s Clinical University Hospital in Riga (Latvia) from 2020 to 2023. The patients comprised 66 boys and 54 girls (M age = 7.1, SD = 4.7, range: 0–17 years) with diverse cancer diagnoses. The parents completed the Latvian version of the Psychosocial Assessment Tool [adapted from PAT 3.1], with five subscales included in the data analysis (Social Support, Child Problems, Family Problems, Stress Reactions, and Family Beliefs). The Family Belief subscale was adjusted specifically to address the unique objectives and research questions of the current study.

Results

Significant correlations were found between children’s psychological problems (e.g., getting upset about medical procedures, hyperactivity, excessive use of electronic devices, etc.) and parental stress reactions after diagnosis and with self-reported symptoms of anxiety and depression. The associations were statistically significant, even after controlling for sociodemographic and medical factors such as diagnosis. The relationship between children’s problems and parental stress reactions was partly mediated by Family Beliefs about illness. The most informative beliefs associated with parental stress symptoms and the family’s psychosocial risk level were identified, and significant results were found according to the main component of beliefs (catastrophic vs. optimistic) that explained 42% of the variance in the Family Beliefs subscale.

Implication

Understanding the habitual responses to stress and identifying the thinking patterns of parents that lead to distorted views and maladaptive coping are essential for customizing personalized interventions to enhance treatment compliance. The Latvian version of PAT is a useful psychosocial screening measure in pediatric oncology settings.
The relationship between cognitive emotion regulation strategies and emotional manipulation among health colleges students: a cross-sectional correlational study
Authors: Alwesmi Mai B. , Bayounes Rasha Mohammed , Binrushaydan Norah Nasser , Alanazi Maha Ahmad , Salem Raghad Mohamed , Alomairi Raghad Ahmad , Albugami Amal Zaid , Alzahrani Ebtisam Mohammed , Alahmari Lama A. , Youssef Naglaa
Published: September 2024

Emotional intelligence is commonly associated with career success. Employees with higher emotional intelligence tend to reap greater benefits. However, emotional manipulation has been reported as a dark side of emotional intelligence, which refers to the self-perceived ability to control the emotions and actions of others for self-benefit. Healthcare professionals with high emotional intelligence often improve the overall patient experience. However, their ability to manipulate emotions can have a detrimental impact on the quality of treatment. This cross-sectional correlational study assessed cognitive emotion regulation strategies (CER) and emotional manipulation among students of health colleges. Two instruments were used to collect the data: 1) the emotional manipulation scale and 2) the cognitive emotion regulation questionnaire. A total of 362 students from health colleges responded to the questionnaire. The students had a moderate tendency to use emotional manipulation (25.09 ± 6.79 out of 50). The most frequently used CER strategies included adaptive strategies such as positive reappraisal with a mean score of 7.45 ± 2.15 out of 10 and maladaptive strategies such as rumination with a mean score of 7.33 ± 2.23 out of 10. The emotional manipulation score had a small but statistically significant negative correlation with two adaptive CER strategies: positive refocusing (r = -0.146, p = 0.005) and focus on planning (r = -0.144, p = 0.006). This study sheds light on the relationship between CER strategies and emotional manipulation, suggesting that poor use of adaptive strategies is associated with emotional manipulation. This finding highlights the importance of designing interventional programs that improve the ability of health colleges students to regulate their cognitive and emotional responses, thus improving their overall well-being and performance as future healthcare workers.
Climate change distress and impairment in Germany
Authors: König Lars , Breves Priska , Linnemann Gesa Alena , Hamer Tim , Suhr Ralf
Published: September 2024

Introduction

Climate change has been widely recognized as one of the most challenging problems facing humanity and it imposes serious mental health threats. It is important, however, to differentiate between the affective experience of distress over climate change and the functional impairments associated with climate change. Such a distinction is crucial because not all negative affective states are pathological, and they might even motivate pro-environmental behavior. Functional impairments, like not being able to work or maintaining social relationships, however, might require immediate treatment. This study assesses climate change distress and climate change impairment within the population of Germany using a population-representative sample. The results identify vulnerable subgroups and thereby can help to facilitate the development of target group specific intervention programs. Furthermore, this study explores whether climate change distress and climate change impairment are associated with general health, physical health, mental health, and diverse health behaviors.

Methods

Study participants were drawn from a panel which is representative of the German-speaking population in Germany with Internet access. Participants answered a series of questionnaires regarding their climate change distress, climate change impairment, general health, physical health, mental health, and diverse health behaviors. To evaluate differences between subgroups, Bayesian independent samples t-tests were calculated. To evaluate associations between constructs, Bayesian correlations were calculated.

Results

Especially women, younger people, people from West Germany, and people with a high level of formal education seem to experience higher levels of climate change distress. Regarding climate change impairment, the results suggest that especially women, older people, people from West Germany, people with a low level of formal education, people with a low or middle social status, and people with an inadequate/problematic health literacy seem to experience higher levels of climate change impairment. Furthermore, climate change distress and climate change impairment were weakly and differently associated with general health, physical health, mental health, and diverse health behaviors.

Discussion

Climate change distress and impairment are not evenly distributed within German society. The results of this study provide a starting point for the development of target group specific intervention programs.
The knowledge, training, and willingness of first year students in Xuzhou, China to perform bystander cardiopulmonary resuscitation: a cross-sectional study
Authors: Qin Zhaohui , Zheng Shuyao , Liu Chenxu , Ren Yuxin , Wang Ran , Zhang Sitian , Gu Xiao , Li Yichen , Yan Xianliang , Xu Tie
Published: September 2024

Background

Bystander Cardiopulmonary Resuscitation (CPR) can significantly improve the rate of return of spontaneous circulation in patients with cardiac arrest. Since first year students with no specific academic background are energetic and quick to learn, many Chinese schools offer first-aid training course including CPR to them before they start school. However, data on CPR knowledge, training, and willingness among first year students are lacking in most regions of China, which makes the effectiveness of CPR training unknown.

Objectives

To evaluate first year students’ knowledge level, training experience and rescue willingness for CPR of first year students in Xuzhou, and to analyze the influencing factors of CPR knowledge level and rescue willingness of first year students in Xuzhou.

Design

A cross-sectional study.

Participations

A total of 9,887 first year students from three universities in Xuzhou city were selected by multi-stage random cluster sampling method.

Methods

A self-designed five-part structured questionnaire was used to investigate the knowledge, training and willingness of CPR among first year students. Independent sample t-test, χ2-test and Logistic regression were used for data analysis.

Results

The average score of CPR knowledge was 2.44 (±1.60), 99.13% of the respondents were willing to participate in CPR training, and 66.25% had received CPR training. Respondents with rural household registration, relatives who had suffered from serious diseases, relatives engaged in the medical profession, good self-rated quality of life, CPR training, and willing to CPR training had higher CPR knowledge levels. 76.77% of the respondents were willing to perform bystander CPR. Women, those who had received CPR training, and those who were willing to receive CPR training were more willing to help patients with sudden cardiac arrest. Lack of first aid knowledge and skills (82.61%) was the biggest obstacle hindering respondents from performing rescue.

Conclusion

Most of the first year students of Xuzhou University in China have CPR training experience and have a strong willingness to train. Most are willing to perform bystander CPR, but have a low knowledge level. Colleges and universities can adopt diversified training methods, make plans for regular CPR retraining and other strategies to improve the quality and effect of CPR training for college students.
Remote coaching for supporting the implementation of treatment for depression in primary care in Madhya Pradesh, India: protocol for a cluster randomized controlled trial
Authors: Bondre Ameya P. , Singh Abhishek , Tugnawat Deepak , Chandke Dinesh , Khan Azaz , Shrivastava Ritu , Lu Chunling , Ramaswamy Rohit , Patel Vikram , Bhan Anant , Naslund John A.
Published: September 2024

Background

Upwards of ninety percent of individuals living with depression in India do not have access to evidence-based treatments, especially in rural areas. Integrating these treatments into primary care is essential for bridging this care gap. This trial aims to evaluate whether a remote coaching implementation support strategy, referred to as Enhanced Implementation Support, is superior to routine support, referred to as Routine Implementation Support, in supporting the delivery of collaborative depression care in rural primary care centers.

Methods

Employing a cluster-randomized hybrid type-III implementation trial design, 14 primary care facilities in Sehore district, Madhya Pradesh, will implement a collaborative depression care package based on the WHO's mhGAP program. Facilities will be randomized to either Enhanced Implementation Support or the Routine Implementation Support control condition. Enhanced Implementation Support consists of remote coaching and technical assistance, supplemented with in-person visits, and guided by the Plan-Do-Study-Act implementation cycles. The primary implementation outcome is the proportion of outpatients screened for depression by facility staff, with secondary outcomes including the proportions of outpatients who screen positive for depression, are referred to the medical officer, and initiate treatment. Secondary patient outcomes include proportion of patients who achieve reduction in depression symptom severity at 3-month follow up. Acceptability, feasibility, and fidelity of the depression care package will be assessed through routine observations collected during field visits, facility audits, and qualitative exit interviews with facility staff. Costs of delivering the Enhanced Implementation Support strategy will also be estimated.

Discussion

This trial can inform efforts to integrate depression care in rural primary care facilities in a low-resource setting, and illuminate whether external coaching support is superior relative to existing implementation support for achieving these goals.

Trial Registration

NCT05264792.
Multiparameter analysis of Diuron and its metabolites (DCA and DCPMU) in different stages of zebrafish (Danio rerio) development
Authors: Peixoto Paloma Vitória Lima , Sales Bianca Camargo Penteado , Aggio Luiza , Viriato Cristina , Pereira Lilian Cristina
Published: September 2024

Introduction

Diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a prominent herbicide in Brazilian agriculture, particularly in sugarcane farming. This herbicide persists in the environment and is transformed into potentially toxic metabolites, such as 3,4-dichloroaniline (DCA) and 3-(3,4-dichlorophenyl)-1-methylurea (DCPMU), raising concerns that encompass environmental contamination and One Health, which unites environmental and human health. Zebrafish are an appropriate experimental model to evaluate toxicity: they metabolically and genetically resemble humans and can be applied in ecotoxicology.

Methods

In this study, we have employed multiparameter evaluation to characterize the toxicity of environmentally relevant concentrations (0.5–100 μM) of Diuron, DCA, or DCPMU in Brazil, a developing country. In the early stages of zebrafish development, we performed the fish embryo toxicity test according to the guidelines established in OECD Protocol No. 236, extended to 144 h post fertilization (hpf). We also investigated the oxygen consumption rate in zebrafish embryos at 24 hpf, evaluated the acetylcholinesterase (AChE) enzymatic activity in zebrafish larvae at 96 hpf, and used the Comet Assay to assess DNA fragmentation in zebrafish larvae at 96 hpf. We subjected adult zebrafish to acute exposure for 96 h in accordance with OECD protocol no. 203 for the Acute Toxicity Test in Fish. Following this exposure, we analyzed mutagenicity by using the micronucleus test, which was complemented by quantification of nuclear anomalies (NA) in erythrocytes and further confirmed by histopathological investigations.

Results and discussion

Exposure to any of the tested Diuron concentrations damaged DNA, and NA became significant beyond 10 μM Diuron. At 10 μM, DCA altered AChE activity, suggesting neuromuscular toxicity. Between 0.5 and 5 μM, DCA was potentially genotoxic, and 5–10 μM DCA induced nuclear pleomorphism. At all the tested concentrations, DCPMU damaged DNA, and NA emerged between 1 and 10 μM DCPMU. Adult zebrafish exposed to any of the tested Diuron, DCA, or DCPMU concentrations for 96 h did not exhibit significant histopathological alterations. Erythrocyte morphology analysis did not reveal mutagenicity. Although we observed non-mutagenic genotoxicity, chromosomal instability is of concern. The findings of this study contribute to our understanding of how Diuron and its metabolites affect zebrafish and highlights the need for multiparametric assessment to elucidate how herbicides and their metabolites impact the environment and human health. This endeavor informs regulatory measures for safely using Diuron and similar herbicides and reveals substantial implications for developing nations grappling with escalating water contamination due to pesticide use.
Potential financial loss and risk factors for hamstring muscle injuries in elite male Brazilian soccer players: a season-long prospective cohort pilot study
Authors: Oliveira-Júnior Otaviano , Gabbett Tim J. , Bittencourt Natalia F. N. , Quintão Roberto C. , Reis Guilherme F. , Claudino João G. , Lasmar Rodrigo C. P. , Leopoldino Amanda A. O.
Published: September 2024

Purpose

The aim of this pilot study was to analyze the potential financial loss and a range of potential risk factors for hamstring muscle injuries in elite Brazilian soccer.

Methods

Thirty-four male players (age: 25 ± 6 years; stature: 180 ± 8 cm; body mass: 78 ± 9 kg; minutes played in matches: 2243 ± 1423 min) from an elite professional soccer club were monitored during a 12-month season. Muscle injury was identified by magnetic resonance imaging and the severity was defined according to the number of days away: minimal (1–3 days), mild (4–7 days), moderate (8–28 days), severe (>28 days). Potential financial loss due to the team's under achievements was determined. Dorsiflexion range of motion, eccentric knee flexor strength and isokinetic tests were performed during the pre-season. Association between dependent variables and the occurrence of injury was evaluated.

Results

Nine hamstring muscle injuries with moderate severity were found in 8 athletes. Recovery time was 22 days off the field on average. Potential financial loss was $-43.2 million USD and earnings on merit money was 21%. Previous injury, increased flexor deficit 60° /sec and increased flexor fatigue index 300°/sec were all associated with a greater chance of hamstring muscle injury. Ankle dorsiflexion range of motion was significantly lower in the injured group (35.6 ± 3° vs. 39.1 ± 4.9°; p = 0.017, effect size = −0.74).

Conclusion

High financial burden was found in elite Brazilian soccer during one full season. Injured athletes had high hamstring fatigue index, knee flexor strength deficit, ankle range of motion restriction and previous hamstring muscle injury when compared to non-injured athletes. Therefore, preventive approaches in professional soccer players with previous hamstring injuries should be a priority.
Feature importance for estimating rating of perceived exertion from cardiorespiratory signals using machine learning
Authors: Cheng Runbei , Haste Phoebe , Levens Elyse , Bergmann Jeroen
Published: September 2024

Introduction

The purpose of this study is to investigate the importance of respiratory features, relative to heart rate (HR), when estimating rating of perceived exertion (RPE) using machine learning models.

Methods

A total of 20 participants aged 18 to 43 were recruited to carry out Yo-Yo level-1 intermittent recovery tests, while wearing a COSMED K5 portable metabolic machine. RPE information was collected throughout the Yo-Yo test for each participant. Three regression models (linear, random forest, and a multi-layer perceptron) were tested with 8 training features (HR, minute ventilation (VE), respiratory frequency (Rf), volume of oxygen consumed (VO2), age, gender, weight, and height).

Results

Using a leave-one-subject-out cross validation, the random forest model was found to be the most accurate, with a root mean square error of 1.849, and a mean absolute error of 1.461 ± 1.133. Feature importance was estimated via permutation feature importance, and VE was found to be the most important for all three models followed by HR.

Discussion

Future works that aim to estimate RPE using wearable sensors should therefore consider using a combination of cardiovascular and respiratory data.
Risk map of human intake of mercury through fish consumption in Latin America and the Caribbean
Authors: Vergara Elvira , Pancetti Floria , Zúñiga Liliana , Fabres Karen , Bahamonde Paulina
Published: September 2024

Mercury (Hg) is a persistent pollutant highly bioaccumulated in the aquatic environment through the food chain reaching high concentration levels in the tissues of predator fishes. Among the relevant sources of anthropogenic mercury emissions in Latin America and the Caribbean (LAC), mining is one of the most important along with soil erosion due to deforestation and agricultural activities where pesticides are intensively used. Several reports have demonstrated an association between a fish-based diet with elevated Hg levels in the blood and neurotoxic effects in humans. In this systematic review with quantitative analysis data from 92 articles were compiled, providing evidence of Hg concentration in fishes that are commonly consumed in LAC. An assessment was conducted using three indices for health risk: the Minamata Initial Assessment (MIA), the Target Hazard Quotient (THQ), and the Meals per Week (MPW) index. Of the 410 fish species studied, 5.4% had concentrations above 0.95 μg g−1 of wet weight (ww), which is the recommended limit for Total Hg (THg) ingestion through fish consumption according to the MIA index, regardless of the water habitat (i.e., marine or freshwater). Additionally, the Target Hazard Quotient (THQ) calculation indicated high-risk values (THQ ≥ 1) in 15 out of the 19 countries studied, and very high-risk values (THQ ≥ 10) were obtained from Hg concentrations measured in 5 fish species inhabiting watersheds in Trinidad and Tobago, Suriname and Peru. Finally, recommendations on fish consumption were made based on the MPW index. This study reveals the need for updated biomonitoring studies of Hg concentrations in fish to perform more accurate human health risk analyses.
Employee Engagement and Innovative Work Behavior: The Mediating Role of Knowledge-Sharing Behavior in the United Arab Emirates (UAE) Service Context
Authors: Elamin Abdallah M. , Aldabbas Hazem , Ahmed Ahmed Zain Elabdin , Abdullah Abdulaziz N.
Published: September 2024

The aim of this study was to examine the mediating role of knowledge-sharing behavior (KSB) in the relationship between employee engagement and innovative work behavior (IWB). We collected 193 completed survey responses from employees working in the service sector in the United Arab Emirates (UAE). Drawing on social exchange theory (SET), we employed hierarchical regression to analyze the research framework and the mediation effect. The primary findings indicate a significant positive association between employee engagement and IWB, as well as between employee engagement and KSB. Additionally, there is a significant positive association between KSB and IWB. Furthermore, employee engagement has an indirect effect on IWB via the mediating role of KSB. We recommend further research and practical investigation into how employee engagement contributes to enhancing knowledge-sharing behavior and IWB, ultimately improving organizational performance.
Farmers’ Adoption of Agricultural Nature-Based Solutions in Northeast China: An Extended Theory of Planned Behavior Approach
Authors: Zhang Minglong , Huang Yanfang , Zhang Yunzheng , Nie Fengying , Jia Xiangping
Published: September 2024

The academic field has limited studies on implementing nature-based solutions (NbSs) in Chinese agriculture, especially regarding households’ and farmers’ attitudes toward the acceptance of agricultural NbS technologies. Based on this, this study aims to fill this knowledge gap by examining the factors behind farmers’ agricultural practices of NbS adoption in Northeast China. Accordingly, this paper constructs an extended theoretical framework of planned behavior (TPB) and empirically tests it using partial least squares structural equation modeling (PLS-SEM) on data from 298 farmers in Inner Mongolia. It was found that incorporating institutional trust (IT) into TPB improves the prediction of Chinese farmers’ intention to de-farm behavior. Unlike previous studies, this study identified two indirect pathways influencing farmers’ intention to adopt agricultural NbS technologies: from institutional trust through attitude to intention, and from institutional trust through perceived behavioral control to intention. The findings broaden the research perspective in this area, provide empirical evidence for the application of NbSs in agricultural practices in China and other developing countries, and demonstrate in practice that NbS has been promoted as an essential tool to maximize nature’s ability to provide ecosystem services and create direct economic benefits for farmers through increased yields and reduced costs.
Addressing Rural Decline: China’s Practices in Rural Transformation and Farmers’ Income Growth
Authors: Zhang Deshuo , Lin Qingning , Mao Shiping
Published: September 2024

In the context of global rural decline, fostering endogenous momentum through rural transformation to increase farmers’ incomes is a challenge that nations worldwide must address. This study utilizes the 2022 “China Rural Micro-Economic Data” to construct a multidimensional rural transformation index system at the village level, encompassing demographic, land, industrial, social, digital, and ecological transformations. This study evaluates the levels of rural transformation across 15 surveyed provinces in China. Furthermore, it empirically examines the impact of rural transformation on farmers’ incomes, the underlying mechanisms, and the heterogeneity of different transformation models. The findings are as follows: (1) Coastal economically developed regions exhibit higher levels of rural transformation, while inland agricultural provinces show significant lag; (2) Rural transformation effectively promotes the upgrading of agricultural value chains, increases farmers’ market participation, and enhances their access to financial services, thereby boosting farmers’ incomes; and (3) The impact of different transformation models on farmers’ incomes varies significantly; industrial, social, and demographic transformations contribute most prominently to income growth. While digital transformation shows some positive effects, it remains relatively limited. Land and ecological transformations have yet to demonstrate a significant positive impact on farmers’ incomes.
Sarcocornia fruticosa, a Potential Candidate for Saline Agriculture: Antioxidant Levels in Relation to Environmental Conditions in the Eastern Iberian Peninsula
Authors: Ortega Albero Neus , Vallejo Sardon Sara , Lupuţ Ioan , Boscaiu Monica , Donat-Torres Maria P. , Fita Ana , González-Orenga Sara
Published: September 2024

Sustainable crop production requires an innovative approach due to increasing soil salinisation and decreasing freshwater availability. One promising strategy is the domestication of naturally salt-tolerant plant species with commercial potential. Sarcocornia fruticosa is a highly salt-tolerant halophyte, common in Mediterranean marshes, which may hold promise for biosaline agriculture. This study included 11 populations of this species spread over the territory of the Valencian Community in eastern Spain. Climatic data for each locality were obtained from the nearest meteorological stations. Soil analyses included texture, pH, electroconductivity, organic carbon and organic matter. Biochemical analyses on wild-sampled plant material focused on antioxidant compounds, such as carotenoids, phenolics, flavonoids and proline with malondialdehyde (MDA) used as a marker of oxidative stress. All variables (climatic, edaphic and biochemical) were evaluated together using Principal Component Analysis and Spearman correlation. The results obtained indicated some climatic differences in terms of mean annual precipitation, with a clear N-S gradient and considerable edaphic variability. However, none of the environmental conditions showed a clear correlation with plant biochemical characteristics. Significant differences in the levels of phenolic compounds, flavonoids and MDA between populations were probably due to genetic factors and cannot be explained as a response to environmental conditions.
Long-Term Minimum Tillage and Straw Retention Promote Macroaggregate Formation, Carbon and Nitrogen Sequestration under Wheat-Maize Rotation in Northern China
Authors: Ren Zhijie , Han Xiaojie , Han Zhidong , Tian Wenzhong , Li Junhong , Lv Junjie , Shen Yuanxin , Xie Yingxin , Ma Geng , Li Gezi , Zhao Yanan , Wang Chenyang
Published: September 2024

Conservation tillage is believed to promote soil aggregate stability, carbon (C) and nitrogen (N) sequestration, but the underlying mechanisms remain unclear. In this study, soil samples from an 18-year experiment including conventional tillage with straw removal (CT), deep scarification with straw mulching (DS), and no-tillage with straw mulching (NT) were used to obtain different fractions based on a comprehensive wet-sieving method of aggregate and particle size. The results showed that NT and DS increased soil organic carbon (SOC) and N by 9.3–16.4% and 10.8–25.8%, respectively, in addition to increasing the weight proportion of macroaggregates and the contribution of macroaggregate-associated C and N to total SOC and N. The C change in the total POM accounted for 77.4% and 79.9% of the total SOC increase by NT and DS, while the MAOM only accounted for 29.2% and 25.2%, respectively. Meanwhile, microaggregates-within-macroaggregates accounted for 96.9% and 90.5% of the SOC increase by NT and DS, respectively. The total SOC and N were positively correlated with the C and N of the macroaggregates and subfractions. In conclusion, the formation of macroaggregates drives soil C and N sequestration under conservation tillage, and POM and mM were important functional pools in this process.
Biochar Increases Rice Yield in Soda Saline-Alkali Paddy Fields by Improving Saline-Alkali Stress and Phosphorus Use Efficiency
Authors: Li Xuebin , Che Weikang , Piao Junlong , Song Yang , Wang Xudong , Zhang Yue , Miao Shihao , Wang Hongyue , Xie Liming , Sun Jiayi , Jin Feng
Published: September 2024

Soda saline-alkali significantly hinders rice growth, phosphorus utilization efficiency, and yield formation. The application of biochar can alleviate the adverse effects of saline-alkali stress on crops. However, there is limited research on the interaction between biochar and phosphorus fertilizer concerning ionic accumulation, phosphorus utilization efficiency, and rice yield in soda saline-alkali soils. A two-year field experiment was conducted to study the combined effects of biochar and phosphate fertilizer on ionic accumulation, physiological status, phosphorus utilization efficiency, and rice yield in soda saline-alkali soil. Four treatments were established for the study: NK (225 kg N, 75 kg K ha−1 year−1), NPK (225 kg N, 70 kg P, 75 kg K ha−1 year−1), NK + B [225 kg N, 75 kg K ha−1 year−1, 1.5% biochar (w/w)], and NPK + B [225 kg N, 70 kg P, 75 kg K ha−1 year−1, 1.5% biochar (w/w)]. The findings indicated that the combined application of biochar and phosphorus fertilizer (NPK + B) significantly reduced the Na+ concentration, Na+/K+ ratio, malondialdehyde (MDA), superoxide anion (O2−), and hydrogen peroxide (H2O2) levels in rice plants. Furthermore, it resulted in a significant increase in K+ concentration and elevated the levels of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), proline (Pro), soluble protein (SP), soluble sugar (SS), and acid phosphatase (ACP). The NPK + B treatment exhibited a significant difference compared to the other treatments (p < 0.05). Compared with NK, phosphorus accumulation and phosphorus utilization efficiency under NPK + B were significantly increased (p < 0.05). The average of biomass yield, grain yield, and harvest index of NPK + B, NK + B, and NPK significantly surpassed those of NK by 6.28–12.25%, 19.80–42.13%, and 11.59–24.64%, respectively. Moreover, a significant difference was observed between NPK + B and the other treatments (p < 0.05). Principal component analysis of the two-year mean data revealed a strong positive correlation of 89.5% for PC1 and a minor negative correlation of 4.4% for PC2. Our research findings demonstrate that the combination of biochar and phosphorus fertilizer effectively enhances salt and alkali tolerance in rice plants, resulting in increased yield through improved ionic balance and physiological status.
Spatio-Temporal Dynamics of Soil Penetration Resistance Depending on Different Conservation Tillage Systems
Authors: Jug Danijel , Jug Irena , Radočaj Dorijan , Wilczewski Edward , Đurđević Boris , Jurišić Mladen , Zsembeli Jozsef , Brozović Bojana
Published: September 2024

As conservation tillage becomes one of the foundations of sustainable crop production, important questions arise about its value, which needs to be defined and evaluated. One of the most important indicators of soil compaction is penetration resistance (PR), which comes as a short-term response to the state of soil physics. The objective of this work is to compare different tillage treatments (TT) on soil compaction on silty clay loam Stagnosol and silt Gleysol in the continental part of Croatia. The research included three tillage treatments: ST—conventional tillage, CTD—deep conservation tillage, and CTS—shallow conservation tillage. PR was determined on each soil depth of 5 cm up to 80 cm, and measuring was provided on two measuring dates. The obtained results showed a higher influence of the year factor than TT. In the upper layers (up to 35 cm), PR values between TT were with significant differences, but in most cases below root-limiting critical values, while in deeper soil layers (35–80 cm), we found that penetration values on each tillage treatment begin to stabilize and smooth out in most cases, with similar dynamics on both soil types and measurement dates. In most of the cases, the highest PR was measured for conservation treatments in wetter soil conditions.
Reducing the Sodium Adsorption Ratio Improves the Soil Aggregates and Organic Matter in Brackish-Water-Irrigated Cotton Fields
Authors: Xie Yucai , Ning Huifeng , Zhang Xianbo , Zhou Wang , Xu Peiwen , Song Yinping , Li Nanfang , Wang Xingpeng , Liu Hao
Published: September 2024

The assessment of soil health relies on key parameters such as soil aggregates and organic matter content. Therefore, examining the impact of irrigation water ion composition and variations in salinity on soil aggregates and organic matter is imperative, which is key to developing a theoretical basis for the sustainable utilization of saline water resources, particularly in extremely arid regions. This experiment was conducted to investigate the impact of different irrigation water salinity treatments (T3: 3 g/L, T5: 5 g/L, and T7: 7 g/L) on the root zone soil of cotton fields. Each salinity treatment included three variations of the sodium adsorption ratio (SAR) at S10: 10 (mmol/L)1/2, S15: 15 (mmol/L)1/2, and S20: 20 (mmol/L)1/2. Local freshwater irrigation served as the control, resulting in a total of 10 treatments. Our findings show that the soil Ca2+ and Mg2+ content increased with higher irrigation water salinity but decreased with increasing irrigation water SAR. The relative macroaggregate stability and the content of water-stable macroaggregates and soil organic matter (SOM) decreased as the irrigation water salinity and SAR increased. In comparison to T3S20, T5S10 did not improve the soil Na+ content but significantly increased the soil Ca2+ content by 147.76%, while the water-stable aggregate and SOM saw a notable increase of 7.66% and 9.86%, respectively. Reducing the SAR in brackish water lessens its negative impact on soil aggregates in cotton fields. This is primarily because Ca2+ counteracts the dispersive effect of high Na+ concentrations and promotes aggregate formation. Irrigation water with a salinity of 3 g/L and an SAR of 10 (mmol/L)1/2 positively affected the stabilization of soil aggregates and organic matter.
Anatomy of the Male Reproductive System of Sugar Gliders (Petaurus breviceps)
Authors: Yllera María del Mar , Alonso-Peñarando Diana , Lombardero Matilde
Published: September 2024

The present study provides a detailed macroscopic examination (with some microscopic insights) of the genital apparatus of seven adult and intact male sugar gliders, as well as one castrated individual. The scrotum is pendulous and attached to the ventral abdominal wall, situated in the caudal part of the abdomen and cranial to the cloacal opening. The testes are oval shaped with their long axes oriented vertically. The epididymides are attached along the caudomedial border of their respective testes. The head and tail of the epididymides are in close proximity to the poles of the testes but are not directly attached. The deferent ducts are positioned laterally to their ipsilateral ureter as they run near the dorsal surface of the urinary bladder. The ampulla of the deferent duct is absent. The penis is located post-scrotally, lacks insertion into the bony pelvis, and has a bifid distal end. The crura of the penis originate within the ischiocavernosus muscles, and there are two bulbs of the penis. When flaccid, the entire penis is concealed in the perineal region and externalizes through an orifice situated in the ventral part of the cloaca, traversing its floor towards the cloacal opening. The urethral external orifice is positioned at the point where the bifurcation of the free part of the penis begins. The prostate gland resides in the pelvic cavity and consists of two parts: a macroscopically visible body surrounding the urethra and a microscopically identifiable portion embedded within the walls of the duct. Sugar gliders possess two pairs of bulbous bulbourethral glands, located dorsally and laterally to the rectum, but lack vesicular glands.
Relating Wildlife Camera Trap Data to Tick Abundance: Testing the Relationship in Different Habitats
Authors: Vada Rachele , Zanet Stefania , Occhibove Flavia , Fantini Enrica , Palencia Pablo , Ferroglio Ezio
Published: September 2024

The increase in acarological risk of tick bites is significantly driven by profound changes in landscape, which alter the density and distribution of wildlife that support tick populations. As a result of habitat shifts and land abandonment, which create environments conducive to tick proliferation, the risk of disease transmission to humans and animals is increasing. In this context, it is important to explore tick ecology by applying a comprehensive methodology. In this study, we examined the relationship between wildlife temporal occupancy and tick abundance in two distinct regions: an alpine hunting district and a natural park in the Apennines. For each sampling point, we calculated wildlife temporal occupancy from camera trap pictures and estimated ticks’ abundance from dragging transects in the area immediately surrounding camera traps. In modelling the relationship between those two variables, we included abiotic factors such as saturation deficit, normalized difference vegetation index, and altitude. Results show the importance of altitude and wildlife temporal occupancy (itself related to different habitat and land management characteristics) on the ecology of questing ticks. If employed in management decisions for natural environments, such information is useful to modulate the acarological risk and thus the risk of tick-borne pathogens’ transmission.
Efficacy and Growth Performance between Two Different Ionophore Coccidiostats (Narasin and Salinomycin) in Broiler Chickens after Challenge with Eimeria spp.
Authors: Rogala-Hnatowska Monika , Gould George , Mehrotra Shubhi , Drażbo Aleksandra , Konieczka Paweł , Ramasami Prakash , Kozłowski Krzysztof
Published: September 2024

The objective of this study was primarily to assess the different performance impacts of two ionophore coccidiostats (narasin and salinomycin) used to manage coccidiosis. While both products may be efficacious in controlling disease challenges, previous literature has suggested that some ionophores are less well tolerated by the broiler chickens. In this study, we were particularly interested to know how the use of different coccidiostat programs translates into broiler health and performance, as measured by zootechnical parameters such as the feed conversion ratio, average daily gain, and final body weight. A total of 352 male Ross 308 one-day-old broilers were randomly divided into two treatment groups (T1 and T2). Treatment 1 included a basal diet (BD) + nicarbazin/narasin (Maxiban®, Elanco) at 100 ppm 0–24 days, narasin at 70 ppm 25–42 days, and (2) Treatment 2 included basal diet + nicarbazin/narasin at 100 ppm 0–24 days, salinomycin (Sacox®, Huvepharma) at 70 ppm 25–42 days. Efficacy and performance parameters, slaughter analysis, dry matter (DM) in litter, and intestinal integrity (I2) were measured for the broilers from both treatment groups. The findings demonstrated more favorable results for broilers reared in the group diet fed with narasin (in the finisher phase), including higher daily body weight gain, higher final body weight, lower feed conversion ratio value (improved feed efficiency), and higher European Production Efficiency Factor value, compared with the salinomycin-supplemented group.
Molecular Identification and Engineering a Salt-Tolerant GH11 Xylanase for Efficient Xylooligosaccharides Production
Authors: Ma Jiao , Sun Zhongke , Ni Zifu , Qi Yanli , Sun Qianhui , Hu Yuansen , Li Chengwei
Published: September 2024

This study identified a salt-tolerant GH11 xylanase, Xynst, which was isolated from a soil bacterium Bacillus sp. SC1 and can resist as high as 4 M NaCl. After rational design and high-throughput screening of site-directed mutant libraries, a double mutant W6F/Q7H with a 244% increase in catalytic activity and a 10 °C increment in optimal temperature was obtained. Both Xynst and W6F/Q7H xylanases were stimulated by high concentrations of salts. In particular, the activity of W6F/Q7H was more than eight times that of Xynst in the presence of 2 M NaCl at 65 °C. Kinetic parameters indicated they have the highest affinity for beechwood xylan (Km = 0.30 mg mL−1 for Xynst and 0.18 mg mL−1 for W6F/Q7H), and W6F/Q7H has very high catalytic efficiency (Kcat/Km = 15483.33 mL mg−1 s−1). Molecular dynamic simulation suggested that W6F/Q7H has a more compact overall structure, improved rigidity of the active pocket edge, and a flexible upper-end alpha helix. Hydrolysis of different xylans by W6F/Q7H released more xylooligosaccharides and yielded higher proportions of xylobiose and xylotriose than Xynst did. The conversion efficiencies of Xynst and W6F/Q7H on all tested xylans exceeded 20%, suggesting potential applications in the agricultural and food industries.
USP18 Is Associated with PD-L1 Antitumor Immunity and Improved Prognosis in Colorectal Cancer
Authors: Jifu Cili , Lu Linxia , Ding Jiaxin , Lv Mengjun , Xia Jun , Wang Jingtao , Wang Peijun
Published: September 2024

Background: Compared with conventional chemotherapy and targeted therapy, immunotherapy has improved the treatment outlook for a variety of solid tumors, including lung cancer, colorectal cancer (CRC), and melanoma. However, it is effective only in certain patients, necessitating the search for alternative strategies to targeted immunotherapy. The deubiquitinating enzyme USP18 is known to play an important role in various aspects of the immune response, but its role in tumor immunity in CRC remains unclear. Methods: In this study, multiple online datasets were used to systematically analyze the expression, prognosis, and immunomodulatory role of USP18 in CRC. The effect of USP18 on CRC was assessed via shRNA-mediated knockdown of USP18 expression in combination with CCK-8 and colony formation assays. Finally, molecular docking analysis of USP18/ISG15 and programmed death-ligand 1 (PD-L1) was performed via HDOCK, and an ELISA was used to verify the potential of USP18 to regulate PD-L1. Results: Our study revealed that USP18 expression was significantly elevated in CRC patients and closely related to clinicopathological characteristics. The experimental data indicated that silencing USP18 significantly promoted the proliferation and population-dependent growth of CRC cells. In addition, high USP18 expression was positively correlated with the CRC survival rate and closely associated with tumor-infiltrating CD8+ T cells and natural killer (NK) cells. Interestingly, USP18 was correlated with the expression of various chemokines and immune checkpoint genes. The results of molecular docking simulations suggest that USP18 may act as a novel regulator of PD-L1 and that its deficiency may potentiate the antitumor immune response to PD-L1 blockade immunotherapy in CRC. Conclusions: In summary, USP18 shows great promise for research and clinical application as a potential target for CRC immunotherapy.