bluuebunny/crossref_metadata_2025 huggingface dataset:
Silicon Supplementation Modulates Physiochemical Characteristics to Balance and Ameliorate Salinity Stress in Mung Bean
Authors: Al Murad Musa , Muneer Sowbiya
Published: May 2022

Mung bean is a low-cost high-protein legume that is sensitive to salinity. Salt stress has been demonstrated to be mitigated by silicon (Si). In legumes, the potential for silicon (Si)-mediated abiotic stress reduction has mainly been ignored. Moreover, there is little information on the specific role of comparable Si (sodium silicate) concentrations in salinity stress reduction. As a result, the current study investigated the impact of two distinct Si concentrations (1 and 5 mM) on the physiochemical features of the “mung bean,” one of the most extensively cultivated legumes, when exposed to salinity (10, 20, and 50 mM NaCl). Salinity stress reduced growth variables such as biomass, nodule formation, plant length, height, and photosynthetic measures, which were mitigated by silicon supplementation at 5 mM sodium silicate. The inclusion of silicon increased the expression of photosynthetic proteins such as PSI, PSII, and LHCs under salt stress. Salinity stress also caused oxidative damage in the mung bean in the form of hydrogen peroxide (H2O2) and superoxide radical (O2−), leading in increased lipid peroxidation (MDA) and electrolyte leakage. In contrast, 5 mM sodium silicate tends to scavenge free radicals, reducing lipid peroxidation (MDA) and electrolyte loss. This was linked to significant silica deposition in the leaf epidermis, which eventually functioned as a mechanical barrier in mitigating the deleterious effects of salt stress. Si supplementation also decreased Na+ uptake while increasing K+ uptake. Silicon, specifically 5 mM sodium silicate, was found to minimize salinity stress in mung bean by altering physio-chemical parameters such as photosynthetic machinery, Na+/K+ homeostasis, mechanical barriers, osmolyte production, and oxidative stress.
Exploring the Co-creation Value of Residents to Tourists From the Perspective of Place Attachment and Economic Benefits
Authors: Niu Han-Jen , Chen Mei-Jen
Published: May 2022

Local development enhances the economic capacity and quality of life of the residents and, in particular, attracts tourism to the area. The co-creative value of the residents and the tourists can improve the consensus of the residents on the sustainable development of the place. This study focuses on the factors influencing the co-creation of value between residents and visitors in the Tamsui area near Taipei. The research hypothesis is based on the components of local attachment, economic benefits brought by tourists, environmental costs, social and cultural welfare of the place, life satisfaction of the residents, and the value of co-creation between residents and tourists. A total of 430 questionnaires were collected through a questionnaire survey and statistical data were analyzed using a structural equation model, including descriptive statistical analysis, measurement reliability and validity verification, model fit, and structural model analysis to validate the research hypotheses. The study found that place attachment positively and significantly affects the economic benefits, environmental costs, and socio-cultural welfare of residents about tourists. Resident satisfaction is positively and significantly affected by the environmental costs from visitors and by socio-cultural welfare, but there is no significant impact from economic benefits. Finally, based on the findings of the study, practical recommendations were made for enhancing co-creation value between Tamsui residents and visitors, including enhancing residents’ feelings of place attachment and construction of local social culture and welfare. For the residents of Tamsui, unlike the local government and enterprises, need to be able to create value with tourists in order to have a friendly relationship with them and develop regional tourism in a sustainable manner.
An Agile Systems Modeling Framework for Bed Resource Planning During COVID-19 Pandemic in Singapore
Authors: Lam Sean Shao Wei , Pourghaderi Ahmad Reza , Abdullah Hairil Rizal , Nguyen Francis Ngoc Hoang Long , Siddiqui Fahad Javaid , Ansah John Pastor , Low Jenny G. , Matchar David Bruce , Ong Marcus Eng Hock
Published: May 2022

Background

The COVID-19 pandemic has had a major impact on health systems globally. The sufficiency of hospitals' bed resource is a cornerstone for access to care which can significantly impact the public health outcomes.

Objective

We describe the development of a dynamic simulation framework to support agile resource planning during the COVID-19 pandemic in Singapore.

Materials and Methods

The study data were derived from the Singapore General Hospital and public domain sources over the period from 1 January 2020 till 31 May 2020 covering the period when the initial outbreak and surge of COVID-19 cases in Singapore happened. The simulation models and its variants take into consideration the dynamic evolution of the pandemic and the rapidly evolving policies and processes in Singapore.

Results

The models were calibrated against historical data for the Singapore COVID-19 situation. Several variants of the resource planning model were rapidly developed to adapt to the fast-changing COVID-19 situation in Singapore.

Conclusion

The agility in adaptable models and robust collaborative management structure enabled the quick deployment of human and capital resources to sustain the high level of health services delivery during the COVID-19 surge.
Case Study: Efficacy of Engineering Controls in Mitigating Diacetyl and 2,3-Pentanedione Emissions During Coffee Grinding
Authors: Stanton Marcia L. , McClelland Tia L. , Beaty Michael , Ranpara Anand , Martin Stephen B.
Published: May 2022

Exposure to elevated levels of diacetyl in flavoring and microwave popcorn production has been associated with respiratory impairment among workers including from a severe lung disease known as obliterative bronchiolitis. Laboratory studies demonstrate damage to the respiratory tract in rodents exposed to either diacetyl or the related alpha-diketone 2,3-pentanedione. Respiratory tract damage includes the development of obliterative bronchiolitis-like changes in the lungs of rats repeatedly inhaling either diacetyl or 2,3-pentanedione. In one flavored coffee processing facility, current workers who spent time in higher diacetyl and 2,3-pentanedione areas had lower lung function values, while five former flavoring room workers were diagnosed with obliterative bronchiolitis. In that and other coffee roasting and packaging facilities, grinding roasted coffee beans has been identified as contributing to elevated levels of diacetyl and 2,3-pentanedione. To reduce worker exposures, employers can take various actions to control exposures according to the hierarchy of controls. Because elimination or substitution is not applicable to coffee production facilities not using flavorings, use of engineering controls to control exposures at their source is especially important. This work demonstrates the use of temporary ventilated enclosures around grinding equipment in a single coffee roasting and packaging facility to mitigate diacetyl and 2,3-pentanedione emissions from grinding equipment to the main production space. Concentrations of diacetyl and 2,3-pentanedione were measured in various locations throughout the main production space as well as inside and outside of ventilated enclosures to evaluate the effect of the enclosures on exposures. Diacetyl and 2,3-pentanedione concentrations outside one grinder enclosure decreased by 95 and 92%, respectively, despite ground coffee production increasing by 12%, after the enclosure was installed. Outside a second enclosure, diacetyl and 2,3-pentanedione concentrations both decreased 84%, greater than the 33% decrease in ground coffee production after installation. Temporary ventilated enclosures used as engineering control measures in this study effectively reduced emissions of diacetyl and 2,3-pentanedione at the source in this facility. These findings motivated management to explore options with a grinding equipment manufacturer to permanently ventilate their grinders to reduce emissions of diacetyl and 2,3-pentanedione.
Design for Pandemic Information: Examining the Effect of Graphs on Anxiety and Social Distancing Intentions in the COVID-19
Authors: Luo Jing , Zhang Yaqi , Song Yao
Published: May 2022

To increase public awareness and disseminate health information, the WHO and health departments worldwide have been visualizing the latest statistics on the spread of COVID-19 to increase awareness and thus reduce its spread. Within various sources, graphs are frequently used to illustrate COVID-19 datasets. Limited research has provided insights into the effect of different graphs on emotional stress and ineffective behavioral strategies from a cross-cultural perspective. The result of current research suggests a graph with a high proportion size of the colored area (e.g., stacked area graph) might increase people's anxiety and social distancing intentions; people in collectivist culture might have a high level of anxiety and social distancing intentions; the effect of different graphs on social distancing intentions is mediated by anxiety experienced. Theoretical contribution and practical implications on health communication were also discussed in this study.
Coping Strategy, Life Style and Health Status During Phase 3 of Indian National Lockdown for COVID-19 Pandemic—A Pan-India Survey
Authors: Nagarathna Raghuram , Sharma Manjunath N. K. , Ilavarasu Judu , Kulkarni Ravi , Anand Akshay , Majumdar Vijaya , Singh Amit , Ram Jagat , Rain Manjari , Nagendra Hongasandra R.
Published: May 2022

The implementation of timely COVID-19 pan-India lockdown posed challenges to the lifestyle. We looked at the impact of lifestyle on health status during the lockdown in India. A self-rated scale, COVID Health Assessment Scale (CHAS) was circulated to evaluate the physical health or endurance, mental health i.e. anxiety and stress, and coping ability of the individuals under lockdown. This is a pan-India cross-sectional survey study. CHAS was designed by 11 experts in 3 Delphi rounds (CVR = 0.85) and was circulated through various social media platforms, from 9th May to 31st May 2020, across India by snowball circulation method. CHAS forms of 23,760 respondents were downloaded from the Google forms. Logistic regression using R software was used to compare vulnerable (>60 years and with chronic diseases) with non-vulnerable groups. There were 23,317 viable respondents. Majority of respondents included males (58·8%). Graduates/Postgraduates (72·5%), employed (33·0%), businessmen (6·0%), and professionals (9·7%). The vulnerable group had significantly (OR 1.31, p < 0.001) higher representation of overweight individuals as compared to non-vulnerable group. Regular use of tobacco (OR 1.62, p = 0.006) and other addictive substances (OR 1.80, p = 0.039) showed increased vulnerability. Respondents who consume junk food (OR 2.19, p < 0.001) and frequently snack (OR 1.16, p < 0.001) were more likely to be vulnerable. Respondents involved in fitness training (OR 0.57, p < 0.001) or did physical works other than exercise, yoga, walk or household activity (OR 0.88, p = 0.004) before lockdown were less likely to be vulnerable. Majority had a very good lifestyle, 94.4% never smoked or used tobacco, 92.1% were non-alcoholic, 97.5% never used addictive substances, 84.7% had good eating habits, 75.4% were vegetarians, 82.8% had “good” sleep, 71.7% did physical activities. Only 24.7% reported “poor” coping ability. Depression with somewhat low feeling were more likely to be vulnerable (OR 1.26, p < 0.001). A healthy lifestyle that includes healthy eating, proper sleep, physical activeness and non-addictive habits supports better coping ability with lesser psychological distress among Indian population during lockdown.
Expert-Led Module Improves Non-STEM Undergraduate Perception of and Willingness to Receive COVID-19 Vaccines
Authors: Morra Christina N. , Adkins-Jablonsky Sarah J. , Barnes M. Elizabeth , Pirlo Obadiah J. , Almehmi Sloan E. , Convers Bianca J. , Dang Derek L. , Howell Michael L. , Fleming Ryleigh , Raut Samiksha A.
Published: May 2022

As evidence mounted that existing prevention methods would be insufficient to end the COVID-19 pandemic, it became clear that vaccines would be critical to achieve and maintain reduced rates of infection. However, vaccine-hesitant sentiments have become widespread, particularly in populations with lower scientific literacy. The non-STEM major (called non-major) college students represent one such population who rely on one or more science classes to develop their scientific literacy and thus, become candidates of interest for the success of the COVID-19 vaccine campaign. As these students have fewer opportunities to learn how to identify reputable scientific sources or judge the validity of novel scientific findings, it is particularly important that these skills are included in the science courses offered to non-majors. Two concurrent non-major biology courses (N = 98) at the University of Alabama at Birmingham in Spring 2021 completed Likert questionnaires with open-ended questions prior to and after an expert-led Vaccine Awareness educational intervention addressing vaccine-related concerns. In the module, experts gave presentations about COVID-19 related to microbiology, epidemiological factors, and professional experiences relating to COVID-19. Ten students agreed to participate in post-semester one-on-one interviews. Student interviews revealed that students perceived guest lecturers as providing more information and assurance. Questionnaire data showed an increase in student willingness to accept a COVID-19 vaccine as well as increased student perception of the COVID-19 vaccines as both safe and effective (Wilcoxon Rank Sum Test, p < 0.05). However, the questionnaire data revealed 10 of 98 students remained vaccine-resistant, and these students expressed insufficient research and side effects as leading vaccination concerns. Overall, we show expert-led modules can be effective in increasing non-majors willingness to accept COVID-19 vaccines. Future research should explore the experiences of non-majors and guest lectures, particularly as they relate to vaccination and vaccine concerns.
How the Italian Twitter Conversation on Vaccines Changed During the First Phase of the Pandemic: A Mixed-Method Analysis
Authors: Gesualdo Francesco , Parisi Lorenza , Croci Ileana , Comunello Francesca , Parente Andrea , Russo Luisa , Campagna Ilaria , Lanfranchi Barbara , Rota Maria Cristina , Filia Antonietta , Tozzi Alberto Eugenio , Rizzo Caterina
Published: May 2022

In the context of the European Joint Action on Vaccination, we analyzed, through quantitative and qualitative methods, a random sample of vaccine-related tweets published in Italy between November 2019 and June 2020, with the aim of understanding how the Twitter conversation on vaccines changed during the first phase of the pandemic, compared to the pre-pandemic months. Tweets were analyzed by a multidisciplinary team in terms of kind of vaccine, vaccine stance, tone of voice, population target, mentioned source of information. Multiple correspondence analysis was used to identify variables associated with vaccine stance. We analyzed 2,473 tweets. 58.2% mentioned the COVID-19 vaccine. Most had a discouraging stance (38.1%), followed by promotional (32.5%), neutral (22%) and ambiguous (2.5%). The discouraging stance was the most represented before the pandemic (69.6%). In February and March 2020, discouraging tweets decreased intensely and promotional and neutral tweets dominated the conversation. Between April and June 2020, promotional tweets remained more represented (36.5%), followed by discouraging (30%) and neutral (24.3%). The tweets' tone of voice was mainly polemical/complaining, both for promotional and for discouraging tweets. The multiple correspondence analysis identified a definite profile for discouraging and neutral tweets, compared to promotional and ambiguous tweets. In conclusion, the emergence of SARS-CoV-2 caused a deep change in the vaccination discourse on Twitter in Italy, with an increase of promotional and ambiguous tweets. Systematic monitoring of Twitter and other social media, ideally combined with traditional surveys, would enable us to better understand Italian vaccine hesitancy and plan tailored, data-based communication strategies.
The Effectiveness of the Moving to Emptiness Technique on Clients Who Need Help During the COVID-19 Pandemic: A Real-World Study
Authors: Tao Yanqiang , Chen Yi , Zhou Wen , Lai Lihui , Liu Tianjun
Published: May 2022

With Western therapeutic techniques prevailing in Chinese therapies, some techniques that include Chinese traditional cultural features are required since some cultural factors are not considered in the Western method. Our study introduced a new technique, the moving to emptiness technique (MET), which combines Western structural progress and core factors of Chinese culture. Seventeen therapists treated 107 clients with the MET. Clients reported their target symptoms initially, and therapists helped them transfer invisible symptoms to perceivable stuff and remove their jarring stuff using the psychological emptiness area. At the end of the consultations, we found that MET could eliminate symptoms immediately. By grouping target symptoms according to their frequency, the results showed that clients in the high-frequency symptom group had higher rehabilitation rates than those in the low-frequency symptom group. Additionally, the results of the bereavement group were better than those of the non-bereavement group, indicating that the MET can significantly alleviate clients' target symptoms. In future studies, the replication and stability of the MET can be assessed by integrating questionnaires, experimental designs, and neurological equipment.
Relationship Between Lipid Profiles and Hypertension: A Cross-Sectional Study of 62,957 Chinese Adult Males
Authors: Chen Siwei , Cheng Wenke
Published: May 2022

Background

Patterns of dyslipidemia and incidence of hypertension have been rarely reported in Asian populations with inconsistent findings. To accumulate further evidence in Asian populations, the study aimed to investigate the relationship between lipid profiles and hypertension in Chinese adult males.

Methods

We conducted a cross-sectional study based on the data from the DATADRYAD database. The overall population was divided into hypertensive and non-hypertensive groups based on baseline blood pressure levels. For continuous variables, Mann-Whitney test was performed between two groups, while Kruskal-Wallis and Dunn tests were used among multiple groups. The chi-square test was carried out for dichotomous variables. Spearman's correlation coefficient was employed to assess the association between systolic blood pressure (SBP), diastolic blood pressure (DBP) and lipid profiles, whereas the relationship between lipid profiles and the incidence of hypertension was evaluated using multivariate logistic regression. The Bayesian network (BN) model was adopted to investigate the relationship between clinical characteristics and hypertension, and the importance of related predictor to the incidence of hypertension was obtained to make conditional probability analysis.

Results

Finally, totally 62,957 participants were included in this study. In the lipid profiles, total cholesterol (TC), low-density cholesterol (LDL-c), and non- high-density lipoprotein cholesterol (non-HDL-c) were higher in the hypertensive population (p <0.001). In the fully multivariate model, for every 1 mg/dl increase in TC, LDL-c and non-HDL, the risk of hypertension increased by 0.2% [1.002 (1.001–1.003)], 0.1% [1.001 (1.000–1.002)], and 0.1% [1.001 (1.000–1.002)]. Meanwhile, HDL-c became positively associated with the incidence of hypertension (p for trend < 0.001) after adjusting for the body mass index (BMI), and 1 mg/dl increment in HDL-c increased the risk of hypertension by 0.2% [1.002 (1.000–1.002)] after fully adjusting for multiple variables. Furthermore, the BN showed that the importance of age, BMI, fasting plasma glucose (FPG), and TC to the effect of hypertension is 43.3, 27.2, 11.8, and 5.1%, respectively.

Conclusion

Elevated TC, LDL-c, and non-HDL-c were related to incidence of hypertension in Chinese adult males, whereas triglycerides (TG) was not significantly associated. The relationship between HDL-c and hypertension incidence shifted from no association to a positive correlation after adjusting for the BMI. Moreover, the BN model displayed that age, the BMI, FPG, and TC were strongly associated with hypertension incidence.
Estimating Inner Magnetospheric Radial Diffusion Using a Hybrid-Vlasov Simulation
Authors: George H. , Osmane A. , Kilpua E. K. J. , Lejosne S. , Turc L. , Grandin M. , Kalliokoski M. M. H. , Hoilijoki S. , Ganse U. , Alho M. , Battarbee M. , Bussov M. , Dubart M. , Johlander A. , Manglayev T. , Papadakis K. , Pfau-Kempf Y. , Suni J. , Tarvus V. , Zhou H. , Palmroth M.
Published: May 2022

Radial diffusion coefficients quantify non-adiabatic transport of energetic particles by electromagnetic field fluctuations in planetary radiation belts. Theoretically, radial diffusion occurs for an ensemble of particles that experience irreversible violation of their third adiabatic invariant, which is equivalent to a change in their Roederer L* parameter. Thus, the Roederer L* coordinate is the fundamental quantity from which radial diffusion coefficients can be computed. In this study, we present a methodology to calculate the Lagrangian derivative of L* from global magnetospheric simulations, and test it with an application to Vlasiator, a hybrid-Vlasov model of near-Earth space. We use a Hamiltonian formalism for particles confined to closed drift shells with conserved first and second adiabatic invariants to compute changes in the guiding center drift paths due to electric and magnetic field fluctuations. We investigate the feasibility of this methodology by computing the time derivative of L* for an equatorial ultrarelativistic electron population travelling along four guiding center drift paths in the outer radiation belt during a 5 minute portion of a Vlasiator simulation. Radial diffusion in this simulation is primarily driven by ultralow frequency waves in the Pc3 range (10–45 s period range) that are generated in the foreshock and transmitted through the magnetopause to the outer radiation belt environment. Our results show that an alternative methodology to compute detailed radial diffusion transport is now available and could form the basis for comparison studies between numerical and observational measurements of radial transport in the Earth’s radiation belts.
The Road to Technical Proficiency in Cytoreductive Surgery for Peritoneal Carcinomatosis: Risk-Adjusted Cumulative Summation Analysis
Authors: Santullo Francesco , Abatini Carlo , Attalla El Halabieh Miriam , Ferracci Federica , Lodoli Claudio , Barberis Lorenzo , Giovinazzo Francesco , Di Giorgio Andrea , Pacelli Fabio
Published: May 2022

Background

Cytoreductive surgery (CRS) is a technically demanding procedure, and there is considerable debate about its safe application. This study investigated the learning curve for CRS and the clinical outcomes of consecutive patients treated by a single surgeon at a single institution.

Methods

We collected 251 consecutive patients who underwent CRS for peritoneal metastases by a single surgeon at Fondazione Policlinico Universitario A. Gemelli IRCCS, between January 2016 and December 2020. The learning curve was estimated using the cumulative summation analysis (CUSUM) for operative time (OT). Risk-adjusted CUSUM (RA-CUSUM) charts were developed using a composite variable (surgical failure), defined as the occurrence of at least one of the following events: major postoperative complications (Clavien–Dindo grade ≥3), blood loss ≥500 mL, incomplete cytoreduction. Three learning phases were thus derived from the RA-CUSUM analysis, and were compared in terms of perioperative outcomes.

Results

CUSUM-OT showed that the operation time improved significantly after the 161th case. RA-CUSUM analysis allowed to break the CRS learning curve into three different phases: phase 1, “the learning phase” (cases 1–99), phase 2 “the experienced phase” (cases 100–188), and phase 3, “the mastership phase” (cases 189–251). The rate of major postoperative complications decreased significantly over the three phases (p = 0.019). Operative time decreased significantly as well (p = 0.031) and was significantly shorter in phase 3 with respect to the other two phases (phase 3 vs phase 2: 420 min vs 500 min, p = 0.017; phase 3 vs phase 1: 420 min vs 503 min, p = 0.021). Blood loss consistently decreased throughout the three phases (p = 0.001). The rate of incomplete cytoreduction was significantly lower in phase 3 than in phase 2 (4.8% vs 14.6%, p = 0.043).

Conclusion

The CRS failure rate stabilized after the first 99 cases, and the complete surgical proficiency was achieved after 189 cases. A standardised and mentored learning model is a safer strategy to shorten the learning process, to reduce morbidity and mortality, to improve oncologic outcomes.
The Association Between Dietary Intake and Improvement of LARS Among Rectal Cancer Patients After Sphincter-Saving Surgery-A Descriptive Cohort Study
Authors: Liu Wen , Xia Hai Ou
Published: May 2022

Background

Dietary management was an important strategy for controlling low anterior resection syndrome (LARS) after sphincter-saving surgery, however, the influencing dietary factors of LARS are not completely clear. This study aims at exploring the specific association between perioperative intake of foods and nutrients and the improvement of LARS within the first 6 months after surgery.

Methods

This study applied a prospective cohort design. 210 consecutive patients were admitted in the colorectal surgical ward after the sphincter-saving surgery in a tertiary hospital in China from May to November in 2020. The perioperative food intake was assessed by the food frequency questionnaire, and the bowel symptoms were assessed by the Low Anterior Resection Syndrome Score Scale. The binary logistic regression was used to analyze the collected data.

Results

It was found out that both the intake of oil before surgery and at 6 months after surgery were significantly associated with the improvement of LARS. The average daily intake of livestock and poultry meats and oil during the first 6 months after sphincter-saving surgery were also associated with the improvement of LARS.

Conclusions

The relationship between the intake of Livestock and poultry meats and oil and the improvement of LARS was significant in this study. It provides evidence for medical staff to make up effective interventions of moderating diet to promote the relief of LARS during the first 6 months after sphincter-saving surgery.
Evaluation of Serum AMH, INHB Combined with Basic FSH on Ovarian Reserve Function after Laparoscopic Ovarian Endometriosis Cystectomy
Authors: Tang Yan , Li Yanning
Published: May 2022

Objective

The value of serum AMH, INHB, and bFSH levels in assessing postoperative ovarian reserve function was analyzed by measuring serum anti-Mullerian hormone (AMH), inhibin B (INHB), and basal follicle-stimulating hormone (bFSH) levels in patients after laparoscopic cystectomy for endometrioma.

Methods

From June 2019 to December 2021, 124 patients underwent laparoscopic cystectomy for endometrioma in our hospital were selected, and the serum AMH, INHB, bFSH level, antral follicle count (AFC) of all patients before and after operation were detected and compared. According to the results of postoperative testing, all the patients were divided into normal group (n = 86), diminished ovarian reserve (DOR) group (n = 27), and premature ovarian failure (POF) group (n = 11). Pearson correlation model and subject operating characteristic curve (ROC) were used to analyze the correlation and diagnostic value of serum AMH, INHB and bFSH levels with postoperative ovarian reserve function, respectively.

Results

After operation, the levels of serum AMH, INHB and AFC in the DOR group and POF group decreased compared with those before the operation, and the serum bFSH levels increased (p < 0.05). After operation, the levels of serum AMH, INHB and AFC in DOR group and POF group were lower than those in normal group,and the serum bFSH levels were higher than the normal group; the levels of serum AMH, INHB and AFC in POF group were lower than those in DOR group, and the serum bFSH levels were higher than the DOR group (p < 0.05). Pearson analysis showed that serum AMH and INHB levels were negatively correlated with bFSH, and positively correlated with the number of AFC, the serum bFSH level was negatively correlated with the number of AFC (p < 0.05). The diagnostic values of serum AMH, bFSH, INHB and the combination of the three tests for postoperative abnormal ovarian reserve function were 0.866 (95% CI, 0.801–0.923), 0.810 (95% CI, 0.730–0.890), 0.774 (95% CI, 0.687–0.860) and 0.940 (95% CI, 0.900–0.981), respectively.

Conclusion

Serum AMH and INHB levels decreased and bFSH levels increased in patients after laparoscopic cystectomy for endometrioma, both of which were closely related to postoperative ovarian reserve function, and both could evaluate ovarian reserve function after ovarian cyst debulking, and the combined test could significantly improve the detection rate.
Irradiated Non-replicative Lactic Acid Bacteria Preserve Metabolic Activity While Exhibiting Diverse Immune Modulation
Authors: Porfiri Luca , Burtscher Johanna , Kangethe Richard T. , Verhovsek Doris , Cattoli Giovanni , Domig Konrad J. , Wijewardana Viskam
Published: May 2022

In the recent years, safety concerns regarding the administration of probiotics led to an increased interest in developing inactivated probiotics, also called “paraprobiotics”. Gamma irradiation represents a promising tool that can be used to produce safe paraprobiotics by inhibiting replication while preserving the structure, the metabolic activity, and the immunogenicity of bacteria. In this study, we evaluated the ability of four strains of lactic acid bacteria (LAB: Lacticaseibacillus casei, Lactobacillus acidophilus, Lactiplantibacillus plantarum, and Lacticaseibacillus paracasei) in preserving the metabolic activity and the immune modulation of swine porcine peripheral blood mononuclear cells, after gamma irradiation or heat inactivation. Our results show that all four strains retained the metabolic activity following gamma irradiation but not after heat inactivation. In terms of immune-modulatory capacity, irradiated L. acidophilus and Lc. paracasei were able to maintain an overall gene expression pattern similar to their live state, as heat inactivation did with Lc. casei. Moreover, we show that the two inactivation methods applied to the same strain can induce an opposed expression of key genes involved in pro-inflammatory response (e.g., IFNα and interleukin-6 for Lc. casei), whereas gamma irradiation of L. acidophilus and Lc. paracasei was able to induce a downregulation of the anti-inflammatory TGFβ. Taken together, our data show that immune modulation can be impacted not only by different inactivation methods but also by the strain of LAB selected. This study highlights that gamma irradiation harbors the potential to produce safe non-replicative metabolically active LAB and identifies immunomodulatory capacities that may be applied as vaccine adjuvants.
Partial Substitution of Alfalfa Hay by Stevia (Stevia rebaudiana) Hay Can Improve Lactation Performance, Rumen Fermentation, and Nitrogen Utilization of Dairy Cows
Authors: Jiang Maocheng , Datsomor Osmond , Cheng Zhiqiang , Meng Zitong , Zhan Kang , Yang Tianyu , Huang Yinghao , Yan Qi , Zhao Guoqi
Published: May 2022

The objective of this study was to determine the effect of replacing isonitrogenous and isoenergetic basis alfalfa hay (AH) with stevia (Stevia rebaudiana) hay in dairy cow diets on nutrient digestion, milk performance, rumen fermentation, and nitrogen (N) utilization. In this study, 24 healthy Holstein lactating dairy cattle with a similar milk yield of 33.70 ± 2.75 (mean ± SD) kg, days in milk 95.98 ± 23.59 (mean ± SD) days, and body weight 587.75 ± 66.97 (mean ± SD) kg were selected and randomly allocated into three groups. The constituents of the three treatments were (1) 30.0% AH, and 0% stevia hay (SH) for the AH group; (2) 24.0% AH, and 6% SH for the 6% SH group; (3) 18.0% AH, and 12% SH for the 12% SH group. The substitution of AH with SH did not affect dry matter intake (DMI), gross energy (GE), and other nutrients intake but increased the digestibility of neutral detergent fiber (NDF) and acid detergent fiber (ADF). Compared with the AH diet, the cows fed the 6% SH diet had a higher milk yield and concentration of milk fat. Fecal and urinary nitrogen (N) were lower in cows fed a 6% SH diet than in cows fed the AH diet. Milk N secretion and milk N as a percentage of N intake were higher in cows fed a 6% SH diet than in cows fed AH diets. The concentration of ruminal volatile fatty acids, acetic acid, and ammonia-N were higher in cows fed a 6% SH diet than in cows fed an AH diet. By comparison, the 12% SH group did not affect milk yield, milk composition, N utilization, and rumen fermentation compared with the AH and 6% SH groups. In conclusion, it appears that feeding 6% SH, replacing a portion of AH, may improve lactation performance and N utilization for lactating dairy cows.
Prolonged-Release Once-Daily Formulation of Tacrolimus Versus Standard-of-Care Tacrolimus in de novo Kidney Transplant Patients Across Europe
Authors: Budde Klemens , Rostaing Lionel , Maggiore Umberto , Piotti Giovanni , Surace Daniela , Geraci Silvia , Procaccianti Claudio , Nicolini Gabriele , Witzke Oliver , Kamar Nassim , Albano Laetitia , Büchler Matthias , Pascual Julio , Gutiérrez-Dalmau Alex , Kuypers Dirk , Wekerle Thomas , Głyda Maciej , Carmellini Mario , Tisone Giuseppe , Midtvedt Karsten , Wennberg Lars , Grinyó Josep M.
Published: May 2022

Background: Tacrolimus is the calcineurin inhibitor of choice for preventing acute rejection episodes in kidney transplant patients. However, tacrolimus has a narrow therapeutic range that requires regular monitoring of blood concentrations to minimize toxicity. A new once-daily tacrolimus formulation, LCP-tacrolimus (LCPT), has been developed, which uses MeltDose™ drug-delivery technology to control drug release and enhance overall bioavailability. Our study compared dosing of LCPT with current standard-of-care tacrolimus [immediate-release tacrolimus (IR-Tac) or prolonged-release tacrolimus (PR-Tac)] during the 6 months following de novo kidney transplantation. Comparisons of graft function, clinical outcomes, safety, and tolerability for LCPT versus IR-Tac/PR-Tac were also performed.

Methods: Standard immunological risk patients with end-stage renal disease who had received a de novo kidney transplant were randomized (1:1) to LCPT (N = 200) or IR-Tac/PR-Tac (N = 201).

Results: Least squares (LS) mean tacrolimus total daily dose from Week 3 to Month 6 was significantly lower for LCPT than for IR-Tac/PR-Tac. Although LS mean tacrolimus trough levels were significantly higher for LCPT than IR-Tac/PR-Tac, tacrolimus trough levels remained within the standard reference range for most patients. There were no differences between the groups in treatment failure measures or safety profile.

Conclusion: LCPT can achieve similar clinical outcomes to other tacrolimus formulations, with a lower daily dose.

Clinical Trial Registration:https://clinicaltrials.gov/, identifier NCT02432833.
Optimal Intervention for Initial Treatment of Anastomotic Biliary Complications After Right Lobe Living Donor Liver Transplantation
Authors: Kim Min Seob , Hong Suk Kyun , Woo Hye Young , Cho Jae-Hyung , Lee Jeong-Moo , Yoon Kyung Chul , Choi YoungRok , Yi Nam-Joon , Lee Kwang-Woong , Suh Kyung-Suk
Published: May 2022

Graphical Abstract
Hearing Impairments as an Overlooked Condition in Kidney Transplant Recipients
Authors: Simsir Melis , Yildiz Muhammed Gazi , Karatas Murat , Dalgic Abdullah , Ozturk Ilyas , Tatar Erhan , Eren Necmi , Erken Ertugrul , Gungor Ozkan , Altunoren Orcun
Published: May 2022

Graphical Abstract
Progression of AFP SCORE is a Preoperative Predictive Factor of Microvascular Invasion in Selected Patients Meeting Liver Transplantation Criteria for Hepatocellular Carcinoma
Authors: Herrero Astrid , Boivineau Lucile , Cassese Gianluca , Assenat Eric , Riviere Benjamin , Faure Stéphanie , Bedoya José Ursic , Panaro Fabrizio , Guiu Boris , Navarro Francis , Pageaux Georges-Philippe
Published: May 2022

Microvascular invasion (MVI) is one of the main prognostic factors of hepatocellular carcinoma (HCC) after liver transplantation (LT), but its occurrence is unpredictable before surgery. The alpha fetoprotein (AFP) model (composite score including size, number, AFP), currently used in France, defines the selection criteria for LT. This study’s aim was to evaluate the preoperative predictive value of AFP SCORE progression on MVI and overall survival during the waiting period for LT. Data regarding LT recipients for HCC from 2007 to 2015 were retrospectively collected from a single institutional database. Among 159 collected cases, 34 patients progressed according to AFP SCORE from diagnosis until LT. MVI was shown to be an independent histopathological prognostic factor according to Cox regression and competing risk analysis in our cohort. AFP SCORE progression was the only preoperative predictive factor of MVI (OR = 10.79 [2.35–49.4]; p 0.002). The 5-year overall survival in the progression and no progression groups was 63.9% vs. 86.3%, respectively (p = 0.001). Cumulative incidence of HCC recurrence was significantly different between the progression and no progression groups (Sub-HR = 4.89 [CI 2–11.98]). In selected patients, the progression of AFP SCORE during the waiting period can be a useful preoperative tool to predict MVI.
Treatment With Diflunisal in Domino Liver Transplant Recipients With Acquired Amyloid Neuropathy
Authors: Nedkova-Hristova Velina , Baliellas Carmen , González-Costello José , Lladó Laura , González-Vilatarsana Emma , Vélez-Santamaría Valentina , Casasnovas Carlos
Published: May 2022

Graphical Abstract
Impact of Government Policies on Research and Development (R&D) Investment, Innovation, and Productivity: Evidence from Pesticide Firms in China
Authors: Hu Ruifa , Yu Changxin , Jin Yanhong , Pray Carl , Deng Haiyan
Published: May 2022

China’s pesticide industry has played an important role in the growth of agricultural productivity in both China and worldwide, but its development is hampered by production inefficiency and the lack of innovation of active ingredients. To improve innovation and the productivity of the pesticide industry, the Chinese government has implemented a series of policies to stimulate private research and development (R&D) and firm innovation. Using the firm-level panel data of the Annual Survey of Industrial Firms (ASIF) collected by the National Bureau of Statistics in 2001–2007, this study examines the linkages between R&D investment, innovation, and productivity with a focus on the role of government policies. The results show that pesticide firms with a higher intensity of R&D investment were associated with a higher patent intensity, and more innovated firms were associated with a higher productivity. Public research, intellectual property enforcement, production subsidy, foreign direct investment (FDI), and being export oriented were positively associated with the innovation and productivity of pesticide firms.
High-Vigor Seeds Associated with Seed Hardness and Water Absorption Rate in Rice (Oryza sativa L.)
Authors: Li Zhenan , Zhang Tianyu , Zhu Minyi , Li Chengyong , Li Bin , Lu Xiujuan , Wang Jianhua , Jia Liangquan , Qi Hengnian , Wang Xiaomin , Zhao Guangwu
Published: May 2022

Seed physical properties are stable and visualized, and believed to be reference indicators for screening high-vigor seeds. However, the detailed relationship between seed vigor and its physical properties is not fully understood in rice. To elucidate the relationship mentioned above, seed physical properties such as seed size, hardness, and water absorption rate, and seed vigor indicators including germination rate, salt-stressed germination rate, and drought-stressed germination rate were determined among different rice cultivars. Significant differences in seed vigor indicators and seed physical properties were recorded among different rice cultivars. Germination rate, salt-stressed germination rate, drought-stressed germination rate, seed hardness, and water absorption rate ranged from 32.0 ± 1.7% to 99.7 ± 0.3%, 14.4 ± 2.4% to 99.7 ± 0.3%, 3.3 ± 2.6% to 95.7 ± 2.1%, 69.15 ± 0.15 N to 74.56 ± 0.14 N, and 0.09 ± 0.00 g/h to 0.12 ± 0.00 g/h, respectively. Additionally, correlation analysis showed that seed hardness and water absorption rate were significantly positively related to seed vigor (r = 0.33 **−0.41 **, from 2014 to 2016; r = 0.45 **−0.65 **, in 2021). Moreover, principal component analysis determined that the first principal component explained 91.4%, 90.1%, and 89.9% of the variance of seed physical properties, respectively, and loaded on seed hardness and water absorption rate. These results indicate that seed hardness and water absorption rate can be recommended as efficient indicators for screening rice seeds with high vigor.
Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for Ananas comosus L. Merr. Hybrid “MD2”
Authors: Kessel-Domini Argelys , Pérez-Brito Daisy , Guzmán-Antonio Adolfo , Barredo-Pool Felipe A. , Mijangos-Cortés Javier O. , Iglesias-Andreu Lourdes Georgina , Cortés-Velázquez Alberto , Canto-Flick Adriana , Avilés-Viñas Susana A. , Rodríguez-Llanes Yaritza , Santana-Buzzy Nancy
Published: May 2022

The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (Ananas comosus L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants. Ten treatments, with different concentrations of 2,4-Dichlorophenoxyacetic (2,4-D) and Picloram (P), in the absence or presence of 6-Benzylaminopurine (BAP), were used for in vitro morphogenesis induction, as well as histological and molecular techniques, in order to characterize the morphogenic responses induced. Significant differences between treatments tested, to induce callus and buds, were assessed by the Kruskal Wallis method and the Mann–Whitney U-tests. Different pineapple regeneration routes were identified, showing the high regeneration potential of this species. The medium containing 2 mg L−1 2,4-D and 2 mg L−1 BAP, where indirect somatic embryogenesis occurred, was selected as the most efficient treatment, with an average of 120 somatic embryos per explant, differing significantly from the rest of the treatments. It was also demonstrated that the pineapple plants regenerated in vitro preserved the genetic identity of the donor genotype, which represents a high degree of confidence for the application of indirect somatic embryogenesis for A. comusus clonal propagation.
Impact of Population Aging and Renewable Energy Consumption on Agricultural Green Total Factor Productivity in Rural China: Evidence from Panel VAR Approach
Authors: Li Houjian , Zhou Xiaolei , Tang Mengqian , Guo Lili
Published: May 2022

China is moving toward the important goal of being a green and low-carbon country, and the current severity level of population aging is of particular concern to the government. Aging, renewable energy consumption, and technological progress are closely linked. In this research, a panel vector autoregressive (PVAR) model is employed to investigate the long-run equilibrium relationship between population aging, renewable energy consumption and agricultural green total factor productivity using panel data for 30 Chinese provinces (cities) from 2000 to 2019. The findings reveal that, in the long run, both population aging and renewable energy use have considerable positive impacts on agricultural green total factor productivity. In addition, in order to more intuitively understand the impact of population aging and renewable energy consumption on agricultural green total factor productivity, the analysis adopts the impulse response function and variance decomposition. The contributions of population aging and renewable energy consumption to agricultural green total factor productivity are 2.23% and 0.56%, respectively, when the lag period is chosen to be 15, which implies that population aging and renewable energy use will continuously contribute to agricultural green total factor productivity. The study results have significant theoretical implications for understanding China’s aging population structure and current renewable energy use. Given the above results, this study puts forward countermeasures and suggestions from four aspects: improving agricultural infrastructure, increasing agricultural technology investment, increasing the stock of agricultural human capital and strengthening international cooperation.
A Fast Neural Network Based on Attention Mechanisms for Detecting Field Flat Jujube
Authors: Li Shilin , Zhang Shujuan , Xue Jianxin , Sun Haixia , Ren Rui
Published: May 2022

The efficient identification of the field flat jujube is the first condition to realize its automated picking. Consequently, a lightweight algorithm of target identification based on improved YOLOv5 (you only look once) is proposed to meet the requirements of high-accuracy and low-complexity. At first, the proposed method solves the imbalance of data distribution by improving the methods of data enhancement. Then, to improve the accuracy of the model, we adjust the structure and the number of the Concentrated-Comprehensive Convolution Block modules in the backbone network, and introduce the attention mechanisms of Efficient Channel Attention and Coordinate Attention. On this basis, this paper makes lightweight operations by using the Deep Separable Convolution to reduce the complexity of the model. Ultimately, the Complete Intersection over Union loss function and the non-maximum suppression of Distance Intersection over Union are used to optimize the loss function and the post-processing process, respectively. The experimental results show that the mean average precision of improved network reaches 97.4%, which increases by 1.7% compared with the original YOLOv5s network; and, the parameters, floating point of operations, and model size are compressed to 35.39%, 51.27%, and 37.5% of the original network, respectively. The comparison experiments are conducted around the proposed method and the common You Only Look Once target detection algorithms. The experimental results show that the mean average precision of the proposed method is 97.4%, which is higher than the 90.7%, 91.7%, and 88.4% of the YOLOv3, YOLOv4, and YOLOx-s algorithms, and the model size decreased to 2.3%, 2.2%, and 15.7%, respectively. The improved algorithm realizes a reduction of complexity and an increase in accuracy, it can be suitable for lightweight deployment to a mobile terminal at a later stage, and it provides a certain reference for the visual detection of picking robots.