bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Anticancer Activity of 7MeERT over Ertredin: A Comparative Study on Cancer Cell Proliferation and NDUFA12 Binding
Authors: Atsumi Sonoko , Nosaka Chisato , Onodera Takefumi , Adachi Hayamitsu , Watanabe Takumi , Kawada Manabu , Shibuya Masabumi , Park Se In , Kwon Ho Jeong
Published: September 2024

We have previously identified Ertredin (3-(2-amino-5-bromophenyl) quinoxalin-2(1H)-one) as a compound that suppresses 3D spheroid formation and tumorigenesis in NIH3T3 cells induced by Epidermal Growth Factor Receptor variant III (EGFRvIII) transduction. One of its targets has been shown to be NDUFA12 (NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex Subunit 12), a component protein of oxidative phosphorylation complex I. In this report, we compared the growth inhibitory activity of Ertredin with its methylated analogue 7MeERT (3-(2-amino-5-bromophenyl)-7-methylquinoxalin-2(1H)-one) on human cancer cells. 7MeERT induced the inhibition of the proliferation of various cancer cells similarly to Ertredin and showed higher activity in glioblastoma cells, A431 cells overexpressing EGFR (wild type), and multiple myeloma cells. Molecular docking analysis and a Cellular Thermal Shift Assay (CETSA) suggested that 7MeERT binds to NDUFA12 similarly to Ertredin. The binding of 7MeERT and Ertredin to NDUFA12 in glioblastoma was further supported by the inhibition of the oxygen consumption rate. These results suggest that 7MeERT also binds to NDUFA12, inhibits oxidative phosphorylation, and has a higher anti-cancer cell growth inhibitory activity than Ertredin.
Efficacy of IS Code Provisions for Design of Eccentrically Loaded Single Angle Compression Members
Authors: Vivek Sai Kota , Dar Mohammad Adil , Narayanan Subramanian
Published: September 2024

In practical applications, steel single angles are often subjected to eccentric loading when connected to a gusset plate through one leg only, resulting in complex compression behaviour. This behaviour has not been as extensively studied as that of concentrically loaded members. Various international codes of practice offer differed approaches for designing these elements. The literature indicates that the Indian Standard Code IS 800–2007 accurately predicts the axial capacity of eccentrically loaded single angle columns. However, a recent amendment to IS 800 in 2024 introduced modified design provisions. This study is the first to explore the implications of these latest design provisions by comparing them with earlier provisions and experimental strengths reported in the literature. Initially, the design strengths as per the previous and latest design provisions for various Indian Standard Angles (ISA) were presented and compared accounting for varying slenderness ratio, plate slenderness ratio (b/t), and different types of end connections and restraints. The findings reveal that the latest design provisions generally result in much higher design strengths compared to the earlier provisions, with a maximum increase of 104.84%. Upon noticing the significant variation, this study is further extended to compare with the reported data available in the literature. The nominal strengths calculated using the latest provisions were often higher than the strengths reported in the literature considered in this study, indicating potential unsafe design.
Direct Strength Design of Circular Cylindrical Steel Shells under Uniform External Pressure
Authors: Manera Rodrigo da Silva , Basaglia Cilmar , Camotim Dinar
Published: September 2024

Based on a systematic literature review, no design guidelines addressing practical issues related to steel circular hollow section (CHS) members under hydrostatic pressure have been documented thus far, except for the design recommendations proposed by the European standard EN 1993-1-6 concerning the strength and stability of shell structures. Considering that the existing codes are often outdated and subject to improvement, there is a growing demand for modern solutions that can accurately replicate the stability loss response of equipment subject to external pressure. The development of a more direct and simple method, augmented by reliable computational techniques, is advisable to avoid the numerous challenges associated with manual calculations. This approach will streamline the design process and mitigate the complexity of current practical or technical approaches. Thus, this research has formulated a method to predict design strength through the direct application of the critical elastic buckling stress, determined from Generalised Beam Theory (GBT) analyses, induced by hydrostatic pressure in custom-crafted equations, finely tuned for this specific objective. The authors present a formulation of GBT to analyze the elastic buckling behavior of CHS subject to both combined axial compression and external pressure. This work provides an extensive study of the behavior of circular cylindrical shells under external loading conditions. It first introduces the analyses of GBT and the theoretical and empirical formulas applicable to this objective. It then describes the steps involved in the development and validation of a numerical model that simulates the response of circular cylindrical shells under hydrostatic pressure, conducting an extensive parametric study using finite element analyses (FEAs). The results from available tests validate the finite element (FE) model. Upon achieving adequate agreement between the generated numerical and experimental results, new resistance reduction curves for cylindrical shell members are developed based on the results of the parametric study. Additionally, the study compares the existing provisions of EN 1993-1-6 for the design of cylindrical shells under hydrostatic pressure. Finally, a statistical approach is adopted to compare the responses of cylindrical shells calculated according to the EN 1993-1-6 code recommendations with those obtained through the newly proposed method.
Tensile Deformability of Shotcrete in Tunnel Primary Support: A Case Study
Authors: Sun Shunxian , Tian Haiguang , Zhang Zhanjun , Diao Zhaoke , Deng Longhua , Yang Xuxu , Li Chunmeng
Published: September 2024

Shotcrete strain in the primary support of a tunnel will produce non-loading strain at an early age due to the influence of its own temperature change, hardening shrinkage, spraying force, and other factors, which means that current strain-monitoring results fail to reflect the real strain, and the strain value after stabilization is high. In addition, tensile strain may be evident in the final result, even exceeding the tensile warning value, but, in actuality, the on-site lining is very stable, with no cracks or damage. Therefore, it is necessary to understand the strain characteristics of shotcrete in the primary support of a tunnel. Based on the long-span tunnel project at Shishan Road Station on the Qingdao Metro Line 6, in situ and indoor pull tests of concrete strain were designed while only considering temperature change, hardening shrinkage, and spraying force. This study shows the following: (1) The strain in shotcrete is greatly affected by temperature changes, hardening shrinkage, and shotcrete force in the first three days, reaching its peak value in the second to third days, while tending to be stable at about the seventh day. (2) The real strain of the shotcrete was tested, and the warning value was adjusted from 90 με to 120 με. (3) The strain value at the third day was taken as the initial value, and the previous monitoring results were revised. The revised results align with the trends shown during real tests performed on-site, providing guidance for tunnel engineering support monitoring.
Comprehensive Review of the Advancements, Benefits, Challenges, and Design Integration of Energy-Efficient Materials for Sustainable Buildings
Authors: Alassaf Yahya
Published: September 2024

Energy-efficient materials are essential in buildings to reduce energy consumption, lower greenhouse gas emissions, and enhance indoor comfort. These materials help address the increasing energy demand and environmental impact of traditional construction methods. This paper presents a comprehensive literature review that explores advanced materials and technologies for improving building energy efficiency, sustainability, and occupant comfort. The study applies a comparative analysis of peer-reviewed research to examine key technologies analyzed include building-integrated photovoltaics, advanced insulating materials, reflective and thermal coatings, glazing systems, phase-change materials, and green roofs and walls. The study highlights the significant energy savings, thermal performance, and environmental benefits of these materials. By integrating these technologies, buildings can achieve enhanced energy efficiency, reduced carbon footprints, and improved indoor comfort. The findings underscore the potential of advanced building materials in fostering sustainable construction practices. The methodology of this review involves collecting, analyzing, summarizing, comparing and synthesizing existing research to draw conclusions on the performance and efficiency of these technologies.
Influencing Factors, Design Methods, and Buoyancy Reduction Measures for Basement Anti-Flotation Engineering
Authors: Yang Meng , Fu Xu , Liu Huanqin , Wang Lintai , Li Qian
Published: September 2024

Basement anti-flotation design is crucial in modern urban construction. An increase in groundwater buoyancy can cause basement structures to uplift, leading to structural instability or even damage. To ensure the stability and safety of underground structures under various hydrogeological conditions, anti-flotation design must comprehensively consider factors such as construction, design, supervision, structure, and hydrology. During construction, improper dewatering measures or unreasonable construction progress may lead to an abnormal rise in groundwater levels, increasing the risk of anti-flotation. Design considerations must include sufficient safety margins, supervision must fully recognize the impact of groundwater, and the structural dead load must be adequate. Anti-flotation stability verification includes both overall and local anti-flotation, involving the calculation of groundwater buoyancy, structural self-weight, and overburden, and selecting appropriate anti-flotation stability safety factors. The assessment and selection of the anti-flotation design water level are also critical. Common anti-flotation measures include adding counterweights, tension-resistant piles, compression and tension-resistant piles, and hydro-pressure reduction methods, while reinforcement and repair methods include epoxy resin grouting and steel plating reinforcement. Through systematic analysis and comprehensive research, scientific basis and technical support are provided for anti-flotation design, enhancing design efficiency and reliability and ensuring the safety and stability of underground spaces. Future research will develop more accurate calculation methods, improve design standards, and explore new anti-flotation technologies and materials.
Novel Approach to Protect Red Revolutionary Heritage Based on Artificial Intelligence Algorithm and Image-Processing Technology
Authors: Yi Junbo , Tian Yan , Zhao Yuanfei
Published: September 2024

The red revolutionary heritage is a valuable part of China’s historical and cultural legacy, with the potential to generate economic benefits through its thoughtful development. However, challenges such as insufficient understanding, lack of comprehensive planning and layout, and limited protection and utilization methods hinder the full realization of the political, cultural, and economic value of red heritage. To address these problems, this paper thoroughly examines the current state of red revolutionary heritage protection and identifies the problems within the preservation process. Moreover, it proposes leveraging advanced artificial intelligence (AI) technology to repair some damaged image data. Specifically, this paper introduces a red revolutionary cultural relic image-restoration model based on a generative adversarial network (GAN). This model was trained using samples of damaged image and utilizes high-quality models to restore these images effectively. The study also integrates real-world revolutionary heritage images for practical application and assesses its effectiveness through questionnaire surveys. The survey results show that AI algorithms and image-processing technologies hold significant potential in the protection of revolutionary heritage.
Research on the Modular Design Method and Application of Prefabricated Residential Buildings
Authors: Luo Xiaoyong , Zheng Xutong , Liao Chao , Xiao Yang , Deng Chao , Liu Siyu , Chen Qi
Published: September 2024

As one of the key ways to realize the industrialization and green development of construction, prefabricated construction is conducive to saving resources and energy and improving labor productivity and quality. Aiming to solve the problem of the lack of standardization in the design of prefabricated residential buildings, which leads to the components not being universally used and the industrial characteristics not being fully embodied, while excessive standardization leads to a lack of personalization and flexibility, the modular design theory is applied to the standardized design of prefabricated residential buildings in this study. The application route of modular design theory in the standardized design is constructed, that is, “system decomposition—module design—module combination”. Taking residential buildings within a height of 54 m as an example, each basic functional module is standardized and combined into standard plans. At the same time, the functional space module design based on modular coordination and the module combination design based on the trinity of “modulus, pattern, and mode” are discussed. This research is of great significance for giving full play to the comprehensive benefits of prefabricated concrete structures in quality improvement, cost reduction, and rapid assembly.
Influence of Freezing Tunnel Excavation on Foundation Settlement of Buildings
Authors: Zhang Jun , Liu Jiguo , Fu Shuoren , Hong Zequn
Published: September 2024

In this paper, the effects of the cement content and excavation speed parameters on the improvement effect of the artificial freezing method and the characteristics of frozen walls are studied by means of a field test, numerical simulation and theoretical model. The optimization effect of the cement content in the artificial freezing method is studied. It is found that 10% is the best content, which can maximize the freezing wall thickness and grouting effect, and promote uniform distribution of the temperature field. At the same time, the influence of the excavation speed on the stress and settlement of the foundation soil is analyzed. It is pointed out that the increase of the excavation speed will aggravate the settlement and stress redistribution, which may threaten the building structure. The evaluation method proposed in this paper verifies that the construction deformation and settlement control are within the safety standards and provides theoretical support and construction guidance for tunnel engineering.
Performance of Asphalt Mixtures Modified with Desulfurized Rubber and Rock Asphalt Composites
Authors: Xie Shengjia , Cheng Zhiqiang , Zhou Yue , Cao Yadong , Wang Tao , Zhang Zhiqiang , Dai Yiqing , Zhang Weihao
Published: September 2024

This study explores the performance of asphalt mixtures modified with North American rock asphalt and desulfurized rubber particles at varying rubber-to-asphalt ratios ranging from 18% to 36% by weight. A comprehensive set of laboratory tests, including high-temperature rutting tests, low-temperature bending tests, indirect tensile tests, and freeze–thaw splitting tests, were conducted to evaluate the modified mixtures. The results indicate that both wet and dry blending methods produce mixtures that meet technical requirements, with the optimal asphalt-to-aggregate ratio determined to be 7.1%. At a rubber-to-asphalt ratio of 18%, the wet blending method slightly improves high-temperature rutting resistance compared to the dry method. However, an increase in rubber content generally enhances rutting resistance regardless of the blending technique. The wet blending method excels in low-temperature crack resistance, possibly due to better rubber dispersion, while an increase in rubber content diminishes crack resistance due to a thinning asphalt film. In terms of fatigue performance, the dry blending method results in significantly longer fatigue life, with a 27% rubber-to-asphalt ratio exhibiting optimal balance. The dry method consistently outperforms the wet method in water stability, and the resistance to water damage increases with rubber content. In conclusion, this study provides valuable insights into optimizing rubber-to-asphalt ratios and blending methods for various application needs, showcasing the benefits of rock asphalt and desulfurized rubber particles in asphalt modification.
Leucocyte Telomere Length and Lung Cancer Risk: A Systematic Review and Meta-Analysis of Prospective Studies
Authors: Fabiani Roberto , Chiavarini Manuela , Rosignoli Patrizia , Giacchetta Irene
Published: September 2024

Although numerous epidemiological studies are available, the relationship between leukocyte telomere length (LTL) and lung cancer risk is still controversial. This systematic review and meta-analysis, performed according to the PRISMA statement and MOOSE guidelines, aims to summarize the evidence and calculate the risk of lung cancer associated with LTL. The literature search was performed on PubMed, Web of Science, and Scopus databases through May 2024. A random-effects model was used to calculate the pooled risk. Heterogeneity was assessed using I2 and Cochran’s Q statistic. Begg’s and Egger’s tests were used to detect publication bias. Based on 8055 lung cancer cases and 854,653 controls (nine prospective studies), longer LTL was associated with a significant 42% increment in all types of lung cancer risk (OR 1.42, 95% CI 1.24–1.63). The effect was even more evident for adenocarcinomas (OR 1.98, 95% CI 1.69–2.31), while no association was observed for squamous cell carcinoma (OR 0.87, 95% CI 0.72–1.06). Significantly, no association was found for current smokers (OR 1.08, 95% CI 0.90–1.30), while it remained high for both never-smokers (OR 1.92, 95% CI 1.62–2.28) and former smokers (OR 1.34, 95% CI 1.11–1.62). No significant publication bias was evidenced. Longer LTL is associated with an increment in lung cancer risk particularly in never-smoker subjects.
Risk of Esophageal and Gastric Cancer in Patients with Type 2 Diabetes Receiving Glucagon-like Peptide-1 Receptor Agonists (GLP-1 RAs): A National Analysis
Authors: Ayoub Mark , Aibani Rafi , Dodd Tiana , Ceesay Muhammed , Bhinder Muhammad , Faris Carol , Amin Nisar , Daglilar Ebubekir
Published: September 2024

Introduction: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are becoming more popular in managing type 2 diabetes mellitus (T2DM). Concerns linger over potential links to malignancies like pancreatic and thyroid cancers, requiring more research to clarify their safety profiles. Additionally, evidence suggests GLP-1 RAs may lower colorectal and pancreatic cancer risk, especially in obese and overweight individuals, indicating a protective effect beyond weight loss. Current studies leave a gap in comprehensively understanding cancer risks associated with GLP-1 RAs, which prompts further research to enhance our understanding of their overall safety. Methods: We queried the US Collaborative Network (63 health care organizations) of the TriNetX research database. Patients with T2DM were identified and divided into two cohorts: patients on GLP-1 RAs and patients not on GLP-1 RAs. We excluded tobacco use and alcohol use disorders, obese patients with a body mass index (BMI) of >25 kg/m2, and those with a family history of gastrointestinal malignancy, infectious mononucleosis, chronic gastritis, pernicious anemia, helicobacter pylori infection, or gastroesophageal reflux disease (GERD). We used a 1:1 propensity score matching (PSM) model using patients’ baseline characteristics, medications, labs, and genetics. We compared the rate of gastric cancer and esophageal cancer at the seven-year mark. Results: A total of 2,748,431 patients with T2DM were identified. Of those, 6% (n = 167,077) were on a GLP-1 RA and 94% (n = 2,581,354) were not on a GLP-1 RA. After PSM, both cohorts included 146,277 patients. Patients with T2DM who were on a GLP-1 RA, compared to those who were not, had a statistically significant lower risk of both gastric cancer (0.05% vs. 0.13%, p < 0.0001) and esophageal cancer (0.04% vs. 0.13%, p < 0.0001) at the seven-year mark. Conclusion: The use of GLP-1 RAs in patients with T2DM does not significantly increase the risk of gastric or esophageal cancer. This finding supports the continued use of GLP-1 analogues as a therapeutic option in managing T2DM, considering their well-established benefits and low risk of complications. Based on the study results, these medications may even have a protective effect against these malignancies.
The Role of Whole-Gland and Focal Cryotherapy in Recurrent Prostate Cancer
Authors: Pio Faozia , Murdock Andeulazia , Fuller Renee E. , Whalen Michael J.
Published: September 2024

Prostate cancer is the most common non-cutaneous malignancy in men, with the majority of newly diagnosed patients eligible for active surveillance. Despite definitive treatment, a considerable percentage of men will experience biochemical recurrence and even regional and distant metastatic recurrence after radiation therapy or radical prostatectomy. Salvage prostatectomy, while oncologically effective, poses significant morbidity with poor functional outcomes. Salvage cryotherapy has emerged as a promising alternative for localized recurrence, demonstrating safety and efficacy. This review examines the oncologic and functional outcomes of whole-gland and focal salvage cryotherapy, including disease-free survival, cancer-specific survival, and overall survival. The crucial role of multiparametric prostate MRI and evolving role of next-generation PSMA-targeted PET imaging are also examined. The comparison of outcomes of cryotherapy to other salvage ablation modalities, such as high-intensity focused ultrasound (HIFU), is also explored.
The Solute Carrier (SLC) Transporter Superfamily as Therapeutic Targets for the Treatment of Head and Neck Squamous Cell Carcinoma
Authors: Cho Sang Yeon , Kang Nam Sook
Published: September 2024

Background: Head and neck squamous cell carcinoma (HNSC) is the most prevalent cancer in the head and neck region, originating from the mucosal epithelium of the oral cavity, pharynx, and larynx. The solute carrier (SLC) transporter superfamily, consisting of over 400 proteins across 65 families, plays a crucial role in cellular functions and presents promising targets in precision oncology. This study aims to analyze the expression of SLC transporters in HNSC and their potential as biomarkers and therapeutic targets. Methods: We leveraged mRNA and protein expression data from The Cancer Genome Atlas (TCGA) and The Human Protein Atlas (HPA) to examine SLC transporter expression in HNSC. Gene Set Enrichment Analysis (GSEA) was conducted to assess the involvement of SLC transporters in various oncogenic pathways. Results: Significant upregulation of SLC transporters was observed in tumor tissues compared to normal tissues, with notable increases in SLC16A3, SLC53A1, SLC25A32, and SLC2A3. This upregulation correlated with poorer overall survival (OS) and disease-specific survival (DSS). GSEA revealed that these transporters are significantly involved in critical oncogenic pathways, including epithelial-mesenchymal transition (EMT), angiogenesis, and hypoxia, which are vital for cancer progression and metastasis. Conclusions: The study identifies SLC transporters as potential biomarkers and therapeutic targets in HNSC. Targeting these transporters with small molecule inhibitors could disrupt essential supply routes for cancer cells, enhancing treatment efficacy and improving patient outcomes. This study paves the way for developing SLC-based target therapies in precision oncology, with the goal of improving survival rates for patients with HNSC.
Robotic Stereotactic Ablative Radiotherapy for Patients with Early-Stage Lung Cancer: Results of an Interim Analysis
Authors: Zygogianni Anna , Koukourakis Ioannis M. , Georgakopoulos John , Armpilia Christina , Liakouli Zoi , Desse Dimitra , Ntoumas Georgios , Simopoulou Foteini , Nikoloudi Maria , Kouloulias Vassilis
Published: September 2024

Background/Objectives: Surgery is the primary treatment for early-stage lung cancer. Patients with medically inoperable lung carcinomas and patients who refuse to undergo surgery are treated with definite radiotherapy. Stereotactic ablative radiotherapy (SABR) is a compelling non-invasive therapeutic modality for this group of patients that confers promising results. Methods: We report an interim analysis of an ongoing trial. Eighty-one patients with medically inoperable early-stage (T1,2N0) lung cancer underwent SABR in our institution. SABR was delivered via the CyberKnife M6 robotic radiosurgery system. The endpoints of the analysis were treatment efficacy and tolerance. Results: There were no acute or late toxicities from the skin or the connective tissue of the thorax. A grade 2/3 lung injury of non-clinical significance was noted in 6% of patients, which was directly related to a higher biologically effective dose (BEDα/β = 3) and larger irradiation lung volumes in both univariate and multivariate analyses. A local control (LC) was achieved in 100% of the patients at the first follow-up, and the projected 24-month local progression-free survival (LPFS) rate was 95%. The projected 24-month disease-specific overall survival (OS) was 94%. Conclusions: High LC and OS rates can be achieved with SABR for early-stage lung cancer, with minimal toxicity. This study continues to recruit patients.
miR-409-3p Regulates IFNG and p16 Signaling in the Human Blood of Aging-Related Hearing Loss
Authors: Jung Junseo , Lee Jeongmin , Kang Hyunsook , Park Kyeongjin , Kim Young Sun , Ha Jungho , So Seongjun , Sung Siung , Yun Jeong Hyeon , Jang Jeong Hun , Choi Seong Jun , Choung Yun-Hoon
Published: September 2024

Presbycusis, also referred to as age-related hearing loss (ARHL), is a multifaceted condition caused by the natural aging process affecting the auditory system. Genome-wide association studies (GWAS) in human populations can identify potential genes linked to ARHL. Despite this, our knowledge of the biochemical and molecular mechanisms behind the condition remains incomplete. This study aims to evaluate a potential protective tool for ARHL treatment by comparing human blood-based target gene-miRNA associations regulated in ARHL. To identify promising target genes for ARHL, we utilized an mRNA assay. To determine the role of miRNA in ARHL, we investigated the expression profile of miRNA in whole blood in ARHL patients with real-time polymerase chain reaction (RT-qPCR). A reporter gene assay was performed to confirm the regulation of candidate genes by microRNA. Through RT-qPCR validation analysis, we finally confirmed the relationship between ARHL and the role of the interferon-gamma (IFNG) gene. This gene can be regarded as an age-related gene. Through gene ontology (GO) analysis, it has been found that these genes are enriched in pathways related to apoptosis. Among them, IFNG induces an inflammatory response, apoptotic cell death, and cellular senescence. We found that miR-409-3p downregulates the expression of the IFNG in vitro. In addition, the downregulation of the IFNG by miRNA 409-3p promoted cell apoptosis and suppressed proliferation. In conclusion, our study produced gene signatures and associated microRNA regulation that could be a protective key for ARHL patients. IFNG genes and miR-409-3p should be investigated for their usefulness as a new biomarker for treatment modality.
Overcoming Antigen Escape and T-Cell Exhaustion in CAR-T Therapy for Leukemia
Authors: Bartoszewska Elżbieta , Tota Maciej , Kisielewska Monika , Skowron Izabela , Sebastianka Kamil , Stefaniak Oliwia , Molik Klaudia , Rubin Jakub , Kraska Karolina , Choromańska Anna
Published: September 2024

Leukemia is a prevalent pediatric cancer with significant challenges, particularly in relapsed or refractory cases. Chimeric antigen receptor T-cell (CAR-T) therapy has emerged as a personalized cancer treatment, modifying patients’ T cells to target and destroy resistant cancer cells. This study reviews the current therapeutic options of CAR-T therapy for leukemia, addressing the primary obstacles such as antigen escape and T-cell exhaustion. We explore dual-targeting strategies and their potential to improve treatment outcomes by preventing the loss of target antigens. Additionally, we examine the mechanisms of T-cell exhaustion and strategies to enhance CAR-T persistence and effectiveness. Despite remarkable clinical successes, CAR-T therapy poses risks such as cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). Our findings highlight the need for ongoing research to optimize CAR-T applications, reduce toxicities, and extend this innovative therapy to a broader range of hematologic malignancies. This comprehensive review aims to provide valuable insights for improving leukemia treatment and advancing the field of cancer immunotherapy.
Archaeological Excavation, Protection, and Display Engineering Design Practice: A Case Study in the Ruins of the Imperial City of the Minyue Kingdom
Authors: Zhou Shihui , Zhang Lei , Chen Yile , Zheng Liang , Lei Nengzhong , Zhang Jiali
Published: September 2024

The Han Dynasty Ruins in Chengcun Village of Wuyishan City, also known as the Ruins of the Imperial City of the Minyue Kingdom, are located on the hilly slope southwest of Chengcun Village, Xingtian Town, Wuyishan City, Fujian Province, China. These are ruins of a Han Dynasty city. Wuyi Mountain’s World Cultural and Natural Heritage Committee declared it a World Heritage Site in 1999. It is also the only imperial city site from the Han Dynasty that has been declared a World Heritage Site in China, and it is the most well-preserved large-scale imperial city site from the Middle Ages on the Pacific Rim. This study used comprehensive archaeological techniques, including archaeological excavation work, site information recording, erosion situation analysis, and geological surveys, to design and implement protective engineering projects in response to existing problems. In this study, the researchers conducted a geological survey of the study area to analyze the topography, rock and soil distribution characteristics, groundwater storage conditions, and geotechnical engineering conditions. At the same time, they explored the preservation status of the site, including the preservation status of the East Gate and the East City Wall, and they analyzed the causes of damage. Finally, the investigation and analysis results guided the design of a site display project, which included safeguarding against collapse and erosion, treating trees and shrubs, and designing the exhibition project for the East Gate. This study provides some practical reference for the excavation and archaeological work of the royal city in the surrounding areas. At the same time, in terms of the technical process of the project, it is also hoped to provide ideas for international ancient city excavation, display, and protection projects.
The Lacquer Craft of the Corridor Coffin (徼道棺) from Tomb No. 2 of Tushan in Eastern Han Dynasty, Xuzhou
Authors: Li Zongmin , Li Xiaojun , Yu Jinyong , Sun Yihua , Geng Jianjun , Zhao Xing
Published: September 2024

Tomb No. 2 of Tushan in Xuzhou is the tomb of King Chu of the Eastern Han Dynasty, and it was an important archaeological discovery in China. The unique placement and crafting techniques of a lacquer coffin that was unearthed from the burial corridor are of significant importance in the study of tombs. In order to characterise the sample’s microstructure, elemental composition, and structural composition, as well as to study the crafting techniques of the coffin in the corridor, a range of analytical techniques were employed, including ultra-depth microscopy, scanning electron microscopy with SEM-EDS, Raman spectroscopy, FTIR, and XRD. The results indicate that the cross-sectional structure of the fragments comprises a pigment layer and a lacquer ash layer, with the latter being further divided into tile ash and bone ash layers. No lacquer film layer was observed. The primary colouring agent in the pigment layer was HgS, which contained a minor quantity of organic binder. The primary component of the tile ash layer was quartz, while the osseous ash layer comprised particles and collagen derived from mammalian bones. The lacquer crafting technique employed in the construction of the coffin was relatively simple and inconsistent with the assumption of it having a noble status. The findings of this research offer experimental data for the identification, preservation, and technical restoration of the corridor coffin in the future.
Research Progress of Marine Anti-Fouling Coatings
Authors: Wu Shaoqian , Wu Shuo , Xing Shilong , Wang Tianshu , Hou Jiabin , Zhao Yuantao , Li Wenge
Published: September 2024

The extended immersion of ships in seawater frequently results in biofouling, a condition characterized by the accumulation of marine organisms such as barnacles and algae. To combat this issue, the application of anti-fouling coatings to the hull surfaces of vessels has emerged as one of the most effective strategies. In response to the increasing global emphasis on environmental sustainability, there is a growing demand for anti-fouling coatings that not only demonstrate superior anti-fouling efficacy but also adhere to stringent environmental standards. The traditional use of organotin-based self-polishing anti-fouling coatings, known for their high toxicity, has been prohibited due to environmental concerns. Consequently, there is a progressive shift toward the development and application of environmentally friendly anti-fouling coatings. This paper reviews the toxicity and application limitations associated with conventional anti-fouling coatings. It provides a comprehensive overview of recent advancements in the field, including the development of novel self-polishing anti-fouling coatings, low surface energy coatings, biomimetic coatings, and nanostructured coatings, each leveraging distinct anti-fouling mechanisms. The paper evaluates the composition and performance of these emerging coatings and identifies key technical challenges that remain unresolved. It also proposes a multi-faceted approach to addressing these challenges, suggesting potential solutions for enhancing the effectiveness and environmental compatibility of anti-fouling technologies. The paper forecasts future research directions and development trajectories for marine anti-fouling coatings, emphasizing the need for continued innovation to achieve both environmental sustainability and superior anti-fouling performance.
Application of the 5th WHO Guidelines for the Diagnosis of Lung Carcinoma in Small Lung Biopsies in a Tertiary Care Center: Is Insecurity of Pathologists for the Accurate Diagnosis Justified?
Authors: Beckert Manuela , Meyer Christian , Papadopoulos Thomas , Levidou Georgia
Published: September 2024

Background/Objectives: The diagnosis of lung carcinoma (LC) is currently performed in small biopsies and according to the WHO classification by using limited stains to spare tissue for molecular testing. This procedure, however, often causes diagnostic uncertainty among pathologists. Methods: In this retrospective analysis, we compared the diagnosis made by these guidelines in 288 lung biopsies with that using more stains, as retrieved from our archive. We also compared the results of p63 and p40 immunoexpression and investigated the diagnostic role of p53/Rb1. Results: In our investigation, we reached a definite diagnosis with a mean number of one stain compared with six stains in the original diagnostic procedure, with a 97.3% concordance rate. Only in the case of metastases, a clear advantage is proven in the use of more stains, especially in the absence of clinical information. We also found a comparable utility of p40 and p63 for the diagnosis of squamous cell carcinoma, despite the higher p63 expression in other histological types. Moreover, normal p53/Rb1 expression could be utilized for the exclusion of small-cell LC. Conclusions: Our study confirms the diagnostic certainty achieved by the suggestions of the WHO classification and justifies the potential insecurity in the absence of adequate communication with the treating clinician.
The Development of a Yolov8-Based Model for the Measurement of Critical Shoulder Angle (CSA), Lateral Acromion Angle (LAA), and Acromion Index (AI) from Shoulder X-ray Images
Authors: Selçuk Turab
Published: September 2024

Background: The accurate and effective evaluation of parameters such as critical shoulder angle, lateral acromion angle, and acromion index from shoulder X-ray images is crucial for identifying pathological changes and assessing disease risk in the shoulder joint. Methods: In this study, a YOLOv8-based model was developed to automatically measure these three parameters together, contributing to the existing literature. Initially, YOLOv8 was used to segment the acromion, glenoid, and humerus regions, after which the CSA, LAA angles, and AI between these regions were calculated. The MURA dataset was employed in this study. Results: Segmentation performance was evaluated with the Dice and Jaccard similarity indices, both exceeding 0.9. Statistical analyses of the measurement performance, including Pearson correlation coefficient, RMSE, and ICC values demonstrated that the proposed model exhibits high consistency and similarity with manual measurements. Conclusions: The results indicate that automatic measurement methods align with manual measurements with high accuracy and offer an effective alternative for clinical applications. This study provides valuable insights for the early diagnosis and management of shoulder diseases and makes a significant contribution to existing measurement methods.
An Innovative Hybrid Model for Automatic Detection of White Blood Cells in Clinical Laboratories
Authors: Aksoy Aziz
Published: September 2024

Background: Microscopic examination of peripheral blood is a standard practice in clinical medicine. Although manual examination is considered the gold standard, it presents several disadvantages, such as interobserver variability, being quite time-consuming, and requiring well-trained professionals. New automatic digital algorithms have been developed to eliminate the disadvantages of manual examination and improve the workload of clinical laboratories. Objectives: Regular analysis of peripheral blood cells and careful interpretation of their results are critical for protecting individual health and early diagnosis of diseases. Because many diseases can occur due to this, this study aims to detect white blood cells automatically. Methods: A hybrid model has been developed for this purpose. In the developed model, feature extraction has been performed with MobileNetV2 and EfficientNetb0 architectures. In the next step, the neighborhood component analysis (NCA) method eliminated unnecessary features in the feature maps so that the model could work faster. Then, different features of the same image were combined, and the extracted features were combined to increase the model’s performance. Results: The optimized feature map was classified into different classifiers in the last step. The proposed model obtained a competitive accuracy value of 95.6%. Conclusions: The results obtained in the proposed model show that the proposed model can be used in the detection of white blood cells.
Introducing the PrimeD Framework: Teacher Practice and Professional Development through Shulman’s View of Professionalism
Authors: Saderholm Jon , Ronau Robert N. , Rakes Christopher R. , Bush Sarah B. , Mohr-Schroeder Margaret J.
Published: September 2024

This paper clarifies and expands the definition of teacher professional practice, grounded in the commonplaces of professionalism outlined by Lee Shulman. We present the Professional Development: Research, Implementation, and Evaluation (PrimeD) framework as a lens for transforming professional development into a practice that engages teachers as professionals. This discussion explores teachers’ roles in both their classrooms and the profession. The inclusion of PrimeD evaluation and research in the development and practice of mathematics teachers addresses Shulman’s professionalism commonplaces. PrimeD was tested as a lens for professionalism in mathematics teacher education programs at four universities. In the study, teachers collaborated as professionals on developing and testing novel ways to approach mathematics lessons. In general, teachers’ efforts to conduct structured experimentation in their lessons were disconnected from traditional views of the role of a teacher. As a result, teachers who did develop and test lesson trials in this PD program did not frequently continue experimentation. Typically, teachers wanted to collaborate on testing classroom activities but did not have resources to do so (e.g., time, collaborative planning). Systemic changes are needed to promote sustainable change, allowing teachers to collaborate and share the results of classroom research.
An Improved Routing Protocol for Optimum Quality of Service in Device-to-Device and Energy Efficiency in 5G/B5G
Authors: Bunu Sanusi Mohammad , Alani Omar Younis , Saraee Mohammad
Published: September 2024

Some challenges when implementing the optimized link state routing (OLSR) protocol on real-life devices and simulators are unmanageable: link quality, rapid energy depletion, and high processor loads. The causes of these challenges are link state processing, unsuitable multipoint relay (MPR) nodes, and information base maintenance. This paper proposes a structured, energy-efficient link sensing and database maintenance technique. The improved OLSR in the paper replaces the OLSRv2’s HELLO, HELLO, and Topology Control (TC) message sequence with a new sequence. MPR nodes are not mandated to broadcast TC messages if the number of nodes and their OLSRv2 addresses remain unchanged after subsequent broadcasts or if no node reported 2-hop symmetric connections. The paper further proposes an MPR selection technique that considers four parameters: node battery level, mobility speed, node degree, and connection to the base station for optimum relay selection. It combines the four parameters into one metric to reduce energy dissipation and control routing overhead. The modifications were implemented in NS-3, and the simulation results show that our improved OLSR outperforms the existing OLSR, OLSRv2 and other improved routing protocols in energy consumption, routing overhead, the packet delivery ratio and end-to-end delay, as compared to the related literature.
The Desmoplakin Phenotype Spectrum: Is the Inflammation the “Fil Rouge” Linking Myocarditis, Arrhythmogenic Cardiomyopathy, and Uncommon Autoinflammatory Systemic Disease?
Authors: D’Elia Saverio , Caputo Adriano , Natale Francesco , Pezzullo Enrica , Limongelli Giuseppe , Golino Paolo , Cimmino Giovanni , Loffredo Francesco S.
Published: September 2024

Myocarditis is an inflammatory condition of cardiac tissue presenting significant variability in clinical manifestations and outcomes. Its etiology is diverse, encompassing infectious agents (primarily viruses, but also bacteria, protozoa, and helminths) and non-infectious factors (autoimmune responses, toxins, and drugs), though often the specific cause remains unidentified. Recent research has highlighted the potential role of genetic susceptibility in the development of myocarditis (and in some cases the development of inflammatory dilated cardiomyopathy, i.e., the condition in which there is chronic inflammation (>3 months) and left ventricular dysfunction\dilatation), with several studies indicating a correlation between myocarditis and genetic backgrounds. Notably, pathogenic genetic variants linked to dilated or arrhythmic cardiomyopathy are found in 8–16% of myocarditis patients. Genetic predispositions can lead to recurrent myocarditis and a higher incidence of ventricular arrhythmias and heart failure. Moreover, the presence of DSP mutations has been associated with distinct pathological patterns and clinical outcomes in arrhythmogenic cardiomyopathy (hot phases). The interplay between genetic factors and environmental triggers, such as viral infections and physical stress, is crucial in understanding the pathogenesis of myocarditis. Identifying these genetic markers can improve the diagnosis, risk stratification, and management of patients with myocarditis, potentially guiding tailored therapeutic interventions. This review aims to synthesize current knowledge on the genetic underpinnings of myocarditis, with an emphasis on desmoplakin-related arrhythmogenic cardiomyopathy, to enhance clinical understanding and inform future research directions.
Revision of the Most Primitive Taxa of the Family Gyrodactylidae (van Beneden et Hesse, 1864) (Platyhelminthes, Monopisthocotyla) Based on ITS rDNA Phylogeny
Authors: Janulewicz Jakub , Pietkiewicz Maciej , Ziętara Marek S.
Published: September 2024

Background: For the past 25 years, the ITS rDNA (ITS1-5.8S-ITS2) of Gyrodactylidae has been crucial for species identification, description, and phylogeny. This family includes 25 genera parasitizing marine and freshwater fish, initially distinguished by morphological differences in attachment and/or male copulatory organs. Gyrodactylus Nordmann, 1832, the most species-rich genus, has approximately 500 described species and an additional 25,000 species suspected. The genus is not monophyletic, and the functionally adaptive nature of morphological diagnostic characters complicates the delimitation of new genera. Methods: A phylogeny based on ITS rDNA was proposed to address these challenges, using only complete sequences of primitive taxa. Fifty-four sequences were aligned with the MUSCLE v5.1 algorithm, creating a 1590 ps long matrix. Maximum Likelihood (ML) and Bayesian Inference (BI) methods with the models TVM+F+G4 and SYM+G4 for ITS1–ITS2 and 5.8S, respectively, were inferred using IQ-TREE v2.3.5 and BEAST v2.7.6.0. Results: The findings revealed eleven main lineages. Four of them are proposed for classification into new genera: Cichlidarus gen. nov., Iraqemembranatus gen. nov., Macracanthus gen. nov., and Rysavyius gen. nov. Elevating the subgenus G. (Gyrodactylus) to genus rank was supported. Conclusions: The presented phylogeny provides a foundation for developing a classification system within Gyrodactylidae that is both reasonable and comprehensive.