bluuebunny/crossref_metadata_2025 huggingface dataset:
Why is manganese so valuable to bacterial pathogens?
Authors: Čapek Jan , Večerek Branislav
Published: February 2023

Apart from oxygenic photosynthesis, the extent of manganese utilization in bacteria varies from species to species and also appears to depend on external conditions. This observation is in striking contrast to iron, which is similar to manganese but essential for the vast majority of bacteria. To adequately explain the role of manganese in pathogens, we first present in this review that the accumulation of molecular oxygen in the Earth’s atmosphere was a key event that linked manganese utilization to iron utilization and put pressure on the use of manganese in general. We devote a large part of our contribution to explanation of how molecular oxygen interferes with iron so that it enhances oxidative stress in cells, and how bacteria have learned to control the concentration of free iron in the cytosol. The functioning of iron in the presence of molecular oxygen serves as a springboard for a fundamental understanding of why manganese is so valued by bacterial pathogens. The bulk of this review addresses how manganese can replace iron in enzymes. Redox-active enzymes must cope with the higher redox potential of manganese compared to iron. Therefore, specific manganese-dependent isoenzymes have evolved that either lower the redox potential of the bound metal or use a stronger oxidant. In contrast, redox-inactive enzymes can exchange the metal directly within the individual active site, so no isoenzymes are required. It appears that in the physiological context, only redox-inactive mononuclear or dinuclear enzymes are capable of replacing iron with manganese within the same active site. In both cases, cytosolic conditions play an important role in the selection of the metal used. In conclusion, we summarize both well-characterized and less-studied mechanisms of the tug-of-war for manganese between host and pathogen.
Using video calls to study children's conversational development: The case of backchannel signaling
Authors: Bodur Kübra , Nikolaus Mitja , Prévot Laurent , Fourtassi Abdellah
Published: February 2023

Understanding children's conversational skills is crucial for understanding their social, cognitive, and linguistic development, with important applications in health and education. To develop theories based on quantitative studies of conversational development, we need (i) data recorded in naturalistic contexts (e.g., child-caregiver dyads talking in their daily environment) where children are more likely to show much of their conversational competencies, as opposed to controlled laboratory contexts which typically involve talking to a stranger (e.g., the experimenter); (ii) data that allows for clear access to children's multimodal behavior in face-to-face conversations; and (iii) data whose acquisition method is cost-effective with the potential of being deployed at a large scale to capture individual and cultural variability. The current work is a first step to achieving this goal. We built a corpus of video chats involving children in middle childhood (6–12 years old) and their caregivers using a weakly structured word-guessing game to prompt spontaneous conversation. The manual annotations of these recordings have shown a similarity in the frequency distribution of multimodal communicative signals from both children and caregivers. As a case study, we capitalize on this rich behavioral data to study how verbal and non-verbal cues contribute to the children's conversational coordination. In particular, we looked at how children learn to engage in coordinated conversations, not only as speakers but also as listeners, by analyzing children's use of backchannel signaling (e.g., verbal “mh” or head nods) during these conversations. Contrary to results from previous in-lab studies, our use of a more spontaneous conversational setting (as well as more adequate controls) revealed that school-age children are strikingly close to adult-level mastery in many measures of backchanneling. Our work demonstrates the usefulness of recent technology in video calling for acquiring quality data that can be used for research on children's conversational development in the wild.
A review of shared control in automated vehicles: System evaluation
Authors: Sarabia Joseba , Marcano Mauricio , Pérez Joshué , Zubizarreta Asier , Diaz Sergio
Published: February 2023

Shared control has gained considerable attention in the automated vehicle field in recent years, both from a theoretical point of view and also with multiple applications. The development of shared control systems was discussed in a previous review, which presented a taxonomy focused on control algorithms. However, it is still necessary to understand how these systems should be assessed in terms of system performance, driver behavior, cooperation, and road safety. This paper aims to review and classify evaluation methods used in recent studies with real drivers. Results of the present review showed that shared control continues to be of interest to researchers of automated vehicles. The methodology for system evaluation has evolved, with more participants, better testing platforms, and a greater number of comparison baselines. To guide the path toward implementing shared control features in commercial vehicles, this review aims to help researchers to perform relevant evaluation studies in future developments.
Cardiovascular toxicity of tyrosine kinase inhibitors during cancer treatment: Potential involvement of TRPM7
Authors: Liu Qing , Li Suyao , Qiu Yuran , Zhang Jiayu , Rios Francisco J. , Zou Zhiguo , Touyz Rhian M.
Published: February 2023

Receptor tyrosine kinases (RTKs) are a class of membrane spanning cell-surface receptors that transmit extracellular signals through the membrane to trigger diverse intracellular signaling through tyrosine kinases (TKs), and play important role in cancer development. Therapeutic approaches targeting RTKs such as vascular endothelial growth factor receptor (VEGFR), epidermal growth factor receptor (EGFR), and platelet-derived growth factor receptor (PDGFR), and TKs, such as c-Src, ABL, JAK, are widely used to treat human cancers. Despite favorable benefits in cancer treatment that prolong survival, these tyrosine kinase inhibitors (TKIs) and monoclonal antibodies targeting RTKs are also accompanied by adverse effects, including cardiovascular toxicity. Mechanisms underlying TKI-induced cardiovascular toxicity remain unclear. The transient receptor potential melastatin-subfamily member 7 (TRPM7) is a ubiquitously expressed chanzyme consisting of a membrane-based ion channel and intracellular α-kinase. TRPM7 is a cation channel that regulates transmembrane Mg2+ and Ca2+ and is involved in a variety of (patho)physiological processes in the cardiovascular system, contributing to hypertension, cardiac fibrosis, inflammation, and atrial arrhythmias. Of importance, we and others demonstrated significant cross-talk between TRPM7, RTKs, and TK signaling in different cell types including vascular smooth muscle cells (VSMCs), which might be a link between TKIs and their cardiovascular effects. In this review, we summarize the implications of RTK inhibitors (RTKIs) and TKIs in cardiovascular toxicities during anti-cancer treatment, with a focus on the potential role of TRPM7/Mg2+ as a mediator of RTKI/TKI-induced cardiovascular toxicity. We also describe the important role of TRPM7 in cancer development and cardiovascular diseases, and the interaction between TRPM7 and RTKs, providing insights for possible mechanisms underlying cardiovascular disease in cancer patients treated with RTKI/TKIs.
Quantitative reconstruction of Early Cretaceous dune morphology in the Ordos paleo-desert and its paleoclimatic implications
Authors: Cao Shuo , Ma Jing , Zhang Laiming
Published: February 2023

Deserts are among the most important terrestrial sedimentary environments and have existed in all periods throughout Earth’s history. In contrast to modern deserts, the ability to quantitatively evaluate the area and desertification degrees of ancient deserts is limited due to the lack of unambiguous proxies. Dune morphology and their spatial arrangements directly reflect the degree of desertification of deserts. Recently, a methodology for obtaining the morphological parameters of dunes was established, although it has not been applied to ancient dunes and deserts in deep time. In the Ordos Basin, both the Early Cretaceous desert and modern deserts (Hobq Desert and Mu Us Desert) were well developed and exposed, which makes the Ordos Basin an ideal place to quantitatively evaluate the paleo-desert based on the proposed methodology. In this study, a total of 698 and 1,490 morphological parameters reflecting the Early Cretaceous desert and modern deserts in the Ordos Basin were obtained, respectively. For the Ordos paleo-desert, our results show that its area was larger and its degree of desertification was more severe than those of modern mid-latitude deserts, including the modern desert in the Ordos Basin, but were similar to those of the modern low-latitude deserts. Our results, therefore, suggest that the Ordos paleo-desert was a non-negligible and large desert during an interval of Earth’s history. Since the other controlling factors, such as regional geography and sources of both the ancient and modern deserts in the Ordos Basin, have remained nearly unchanged, we speculate that it may have been the relatively high temperature, high levels of atmospheric carbon dioxide and relatively low precipitation during the greenhouse period (i.e., Early Cretaceous) that led to the large area and severe degree of desertification of the Ordos paleo-desert.
Progress in salivary glands: Endocrine glands with immune functions
Authors: Shang Yu Feng , Shen Yi Yang , Zhang Meng Chen , Lv Min Chao , Wang Tong Ying , Chen Xue Qun , Lin Jun
Published: February 2023

The production and secretion of saliva is an essential function of the salivary glands. Saliva is a complicated liquid with different functions, including moistening, digestion, mineralization, lubrication, and mucosal protection. This review focuses on the mechanism and neural regulation of salivary secretion, and saliva is secreted in response to various stimuli, including odor, taste, vision, and mastication. The chemical and physical properties of saliva change dynamically during physiological and pathophysiological processes. Moreover, the central nervous system modulates salivary secretion and function via various neurotransmitters and neuroreceptors. Smell, vision, and taste have been investigated for the connection between salivation and brain function. The immune and endocrine functions of the salivary glands have been explored recently. Salivary glands play an essential role in innate and adaptive immunity and protection. Various immune cells such as B cells, T cells, macrophages, and dendritic cells, as well as immunoglobins like IgA and IgG have been found in salivary glands. Evidence supports the synthesis of corticosterone, testosterone, and melatonin in salivary glands. Saliva contains many potential biomarkers derived from epithelial cells, gingival crevicular fluid, and serum. High level of matrix metalloproteinases and cytokines are potential markers for oral carcinoma, infectious disease in the oral cavity, and systemic disease. Further research is required to monitor and predict potential salivary biomarkers for health and disease in clinical practice and precision medicine.
Associations between smoke exposure and osteoporosis or osteopenia in a US NHANES population of elderly individuals
Authors: Hou Wenyuan , Chen Shaoqi , Zhu Caiyu , Gu Yifan , Zhu Lei , Zhou Zhengxin
Published: February 2023

Background

Tobacco exposure is considered to be a risk factor for reduced bone mineral density (BMD), which may result in osteopenia. Cotinine, a metabolite of nicotine, is commonly utilized as a marker of tobacco exposure. Nevertheless, there are limited clinical data on the associations between osteoporosis (OP) or osteopenia and smoking status or serum cotinine level.

Methods

We thoroughly examined the NHANES cross-sectional data from 2005 to 2010, 2013 to 2014, and 2017 to 2018. Multivariate logistic regression models were applied to assess the associations among smoking status and serum cotinine levels as well as OP and osteopenia. The relationships between serum cotinine level and OP and osteopenia were also assessed using the restricted cubic spline (RCS) method.

Results

A total of 10,564 participants were included in this cross-sectional study. The mean age of the study population was 64.85 ± 9.54 years, and the patients were predominantly male (51.9%). We found that the relationships between higher serum cotinine levels (≥3 ng/ml) and the prevalence of osteoporosis (Model 1: OR=2.27 [1.91-2.69]; Model 2: OR=2.03 [1.70-2.43]; Model 3: OR=2.04 [1.70-2.45]; all p for trend <0.001) remained significant after adjustment for covariates by applying the lowest serum cotinine levels (<0.05 ng/ml) as the reference. Similar results were observed for current smokers, who were more likely to develop OP compared with nonsmokers (Model 1: OR=2.30 [1.90-2.79]; Model 2: OR=2.16 [1.77-2.64]; Model 3: OR=2.16 [1.77-2.65]). Moreover, higher serum cotinine levels were found to be strongly and positively correlated with the prevalence of osteopenia (OR=1.60 [1.42-1.80]). A similar relationship was observed between current smokers and the prevalence of osteopenia compared with nonsmokers (OR=1.70 [1.49-1.94]). RCS regression also showed that serum cotinine levels were nonlinearly and positively correlated with OP and osteopenia, with inflection points of 5.82 ng/ml and 3.26 ng/ml, respectively.

Conclusion

This study showed that being a smoker was associated with the prevalence of OP or osteopenia compared with being a nonsmoker and that there was a strong nonlinear positive dose−response relationship between serum cotinine levels and OP and osteopenia.
Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimp
Authors: Wang Sheng , Li Haoyang , Li Qinyao , Yin Bin , Li Sedong , He Jianguo , Li Chaozheng
Published: February 2023

Toll-like receptors (TLR) play a crucial role in the detection of microbial infections in vertebrates and invertebrates. Mammalian TLRs directly recognize a variety of structurally conserved microbial components. However, invertebrates such as Drosophila indirectly recognize microbial products by binding to the cytokine-like ligand Spätzle, which activates signaling cascades that are not completely understood. In this study, we investigated the signaling events triggered by Toll in response to lipopolysaccharide (LPS), a cell wall component of gram-negative bacteria, and Vibrio parahaemolyticus infection in the arthropod shrimp Litopenaeus vannamei. We found that five of the nine Tolls from L. vannamei bound to LPS and the RNAi of LvToll1, LvToll2, LvToll3, LvToll5, and LvToll9 weakened LvDorsal-L phosphorylation induced by V. parahaemolyticus. All nine Tolls combined with MyD88 via the TIR domain, thereby conferring signals to the tumor necrosis factor receptor-associated factor 6 (TRAF6)-transforming growth factor-β activated kinase 1 binding protein 2 (TAB2)-transforming growth factor-β activated kinase 1 (TAK1) complex. Further examination revealed that the LvTRAF6-LvTAB2-LvTAK1 complex contributes to Dorsal-L phosphorylation and nuclear translocation during V. parahaemolyticus infection. Overall, shrimp Toll1/2/3/5/9–TRAF6/TAB2/TAK1–Dorsal cascades protect the host from V. parahaemolyticus infection, which provides a better understanding of how the innate immune system recognizes and responds to bacterial infections in invertebrates.
Characterization of the tumor-infiltrating immune repertoire in muscle invasive bladder cancer
Authors: Benítez Raquel , Yu Katherine , Sirota Marina , Malats Núria , Pineda Silvia
Published: February 2023

Introduction

Muscle-invasive bladder cancer (MIBC) is a heterogeneous disease with several taxonomic molecular subtypes showing different genetic, clinical, and epidemiological profiles. It has been suggested that MIBC-subtypes follow different tumorigenesis pathways playing decisive roles at different stages of tumor development, resulting in distinct tumor microenvironment containing both innate and adaptive immune cells (T and B lymphocytes). We aim to characterize the MIBC tumor microenvironment by analyzing the tumor-infiltrating B and T cell repertoire according to the taxonomic molecular subtypes.

Methods

RNAseq data from 396 MIBC samples included in TCGA were considered. The subtype information was collected from the international consensus taxonomic classification describing six subtypes: Basal/Squamous-like (Ba/Sq), Luminal papillary (LumP), Luminal non-Specify (LumNS), Luminal unstable (LumU), Stroma-rich, and Neuroendocrine-like (NE-like). Using MiXCR, we mapped the RNA read sequences to their respective B-cell receptor (BCR) and T-cell receptor (TCR) clonotypes. To evaluate the BCR and TCR differences among subtypes, we compared diversity measures (richness and diversity) using a Wilcoxon test and we performed a network analysis to characterize the clonal expansion. For the survival analysis stratified by subtypes, Cox regression models adjusted for age, region, and pathological stage were performed.

Results

Overall, we found different patterns of tumor-infiltrating immune repertoire among the different MIBC subtypes. Stroma-rich and Ba/Sq tumors showed the highest BCR and TCR infiltration while LumP showed the lowest. In addition, we observed that the Ba/Sq and Stroma-rich tumors were more clonally expanded than the Luminal subtypes. Moreover, higher TCR richness and diversity were significantly associated with better survival in the Stroma-rich and Ba/Sq subtypes.

Discussion

This study provides evidence that MIBC subtypes present differences in the tumor microenvironment, in particular, the Ba/Sq and the Stroma-rich are related with a higher tumoral-infiltrating immune repertoire, which seems to be translated into better survival. Determining the causes of the different tumoral-infiltrating immune repertoire according to the MIBC molecular subtypes will help to improve our understanding of the disease and the distinct responses to immunotherapy of MIBC.
Traditional healers’ roles, and the challenges they face in the prevention and control of local disease outbreaks and pandemics: The case of the East Gojjam Zone in northwestern Ethiopia
Authors: Gietaneh Wodaje , Simieneh Muluye Molla , Endalew Bekalu , Tarekegn Senay , Petrucka Pammla , Eyayu Dawit
Published: February 2023

Objectives

The main objective of this study was to assess the roles of traditional healers and the challenges they face in the of prevention and control of both local disease outbreaks and the COVID-19 pandemic, with a special emphasis on the work of traditional healers and healing centers in the East Gojjam Zone in northwestern Ethiopia, between 2020 and 2021.

Methods

From 25 February 2021 to 2 May 2021, a mixed-methods study (qualitative techniques combined with a quantitative approach) was carried out. The study was conducted by traditional healers and at healing centers in the East Gojjam Zone. The quantitative sample size was calculated based on the assumption of a single population proportion formula. As part of the qualitative research, levels of data saturation were continuously monitored, and were used to determine what the maximum number of study participants should be. Traditional healers and their clients were the study units for the quantitative component, whereas traditional healthcare providers (of all types) and religious leaders were purposively selected as the study units for the qualitative part. Descriptive and inferential statistical methods of analysis, and narrative- and content-wise methods of analysis, were used for the quantitative and qualitative components of this study, respectively.

Results

The quantitative findings of this study showed that 64.27% of respondents (95% CI 59.53% to 68.74%) had a good awareness of regional disease outbreaks and of the COVID-19 pandemic. Only 9.59% of people had a positive opinion regarding local disease outbreaks, the COVID-19 pandemic, and the preventive and control measures that were employed in response to these (95% CI 7.11% to 12.83%). In addition, this study revealed that a small percentage of participants (i.e., 2.16%) used traditional control and preventive measures in response to the COVID-19 pandemic and local disease outbreaks.

Conclusion

Less than one-tenth of respondents had a favorable attitude toward local disease outbreaks, the current COVID-19 pandemic, and the preventive and control measures that were employed in response to these. In addition, only a small number of study participants had actually used conventional control and preventive measures in response to local disease outbreaks and the COVID-19 pandemic. Nearly two-thirds of respondents had a good understanding of the preventive and control measures that were employed in response to local disease outbreaks and the COVID-19 pandemic.
Language switching and domain-general control in interpreters
Authors: Nour Soudabeh , Struys Esli
Published: February 2023

The purpose of this paper is to examine whether and how training and professional experience in interpreting affect task switching in this bilingual population. In the first experiment, we compared a group of interpreting students to a group of translation students using the bilingual categorization task to assess their domain-specific language switching before and after training. In the second experiment, we added a group of professional interpreters to the participants in experiment 1 to test prepotent response inhibition using the Simon task (domain-general). First, the results showed training-related improvement in the bilingual categorization task in both student groups, indicating a similar effect for translation and interpreting training. Second, both student groups showed better performance on the Simon task compared to professional interpreters, but only on response times and not on accuracy. The correlation analyses of the two tasks in student groups only showed significant correlations between the global RTs and supported the hypothesis that proactive language control may depend more on inhibition than on the switching-specific factor. Considering language background, the lower onset age of L2 acquisition (AOA2) in the interpreting students (compared to the translation students) was significantly correlated with the congruency effect in the Simon task, indicating an impact of language background on domain-general control. Results were discussed in light of the different engaging elements, including task specificity, training length, research method, and participants' linguistic profile.
Evaluation of the sensitivity on mesoscale eddy associated with the sea surface height anomaly forecasting in the Kuroshio Extension
Authors: Jiang Lin , Duan Wansuo , Wang Hui , Liu Hailong , Tao Lingjiang
Published: February 2023

The sensitivity of the sea surface height anomaly (SSHA) forecasting on the accuracy of mesoscale eddies over the Kuroshio Extension region, which was determined by the conditional non-linear optimal perturbation (CNOP) method using a two-layer quasigeostrophic model, is evaluated by adopting multiply realistic marine datasets through an advanced particle filter assimilation method. It is shown that, if additional observations are preferentially assimilated to the sensitive area of mesoscale eddies identified by the CNOP, where the eddies present a clear high- to low-velocity gradient along the eddy rotation, the forecasting skill of the SSHA can be more significantly improved. It is also demonstrated that the forecasts of the SSHA in the region where the large-scale mean flow possesses much stronger barotropic and/or baroclinic instability tend to exhibit stronger sensitivity to the accuracy of the initial field in the sensitive area of mesoscale eddies. Therefore, more attention should be preferentially paid to the assimilation of the additional observations of the mesoscale eddies for the SSHA forecast in the region with a strong velocity shear of ocean circulation. The present study verifies the sensitivity on mesoscale eddies of SSHA forecasts derived by the two-layer quasigeostrophic model using multiply sets of realistic oceanic data, especially including observation and reanalysis data, which further additionally demonstrates the importance of targeted observations of mesoscale eddies to the SSHA forecast in the regions of strong velocity shear of ocean circulation and provides a more credible scientific basis for the field campaign of the targeted observations for mesoscale eddies associated with the SSHA forecasting.
Impact of Piriformospora indica on various characteristics of tomatoes during nickel nitrate stress under aeroponic and greenhouse conditions
Authors: Mahmoodi Nazanin , Movahedi Zahra , Ghabooli Mehdi
Published: February 2023

As an essential nutrient for plant growth, nickel's (Ni) requirement is very low, and its augmented level causes environmental pollution and toxicity. Being a root endophytic fungus, Piriformospora indica (P. indica) can be beneficial to many plants under stress and non-stress conditions, particularly in terms of their improved growth performance. P. indica, as evidenced, enhances tolerance and resistance in most plants once they experience a range of stresses caused by biotic and abiotic factors, e.g., diseases and heavy metals. Against this background, the positive effects of P. indica on the tomato plants under Ni-induced stress (300, 600, and 900 mg L−1) were analyzed in three experiments at labs, at greenhouses, and via aeroponics in this study. The growth traits of the tomato plants, such as root length (RL) and root dry weight (RDW), were accordingly found to be positively boosted in the cases treated with P. indica compared to the non-treated ones. Treating with P. indica also thwarted the negative effects of Ni on some biochemical traits, including anthocyanin (Anth), proline (Pro), catalase (CAT), and glutathione peroxidase (GPx), while significantly minimizing the adverse impacts of this heavy metal at different levels on hydrogen peroxide (H2O2). Despite this, the Ni-stressed plants indicated much better traits in the presence of this fungus, compared with the non-treated ones, in most of the cases measured. Moreover, the photosynthetic pigments, i.e., chlorophyll a and b (Chl a & b) and carotenoid content (Carrot), were significantly higher in the tomato plants treated with P. indica under high Ni-induced stress as compared with the non-treated ones under non-Ni conditions, in which these pigments were low. The pro-production was further observed all through the P. indica inoculation, which could aid the treated plants in becoming Ni-stress-tolerant. Finally, the current study contributed to a better understanding of how to use the P. indica symbiosis to induce heavy metal tolerance in tomato plants, such as Ni, to meet the goals of sustainable agriculture.
Plasma and urinary extracellular vesicles as a source of RNA biomarkers for prostate cancer in liquid biopsies
Authors: Bajo-Santos Cristina , Brokāne Agnese , Zayakin Pawel , Endzeliņš Edgars , Soboļevska Kristīne , Belovs Alberts , Jansons Juris , Sperga Māris , Llorente Alicia , Radoviča-Spalviņa Ilze , Lietuvietis Vilnis , Linē Aija
Published: February 2023

Introduction: Extracellular vesicles (EVs) have emerged as a very attractive source of cancer- derived RNA biomarkers for the early detection, prognosis and monitoring of various cancers, including prostate cancer (PC). However, biofluids contain a mixture of EVs released from a variety of tissues and the fraction of total EVs that are derived from PC tissue is not known. Moreover, the optimal biofluid—plasma or urine—that is more suitable for the detection of EV- enclosed RNA biomarkers is not yet clear.

Methodology: In the current study, we performed RNA sequencing analysis of plasma and urinary EVs collected before and after radical prostatectomy, and matched tumor and normal prostate tissues of 10 patients with prostate cancer.

Results and Discussion: The most abundant RNA biotypes in EVs were miRNA, piRNA, tRNA, lncRNA, rRNA and mRNA. To identify putative cancer-derived RNA biomarkers, we searched for RNAs that were overexpressed in tumor as compared to normal tissues, present in the pre-operation EVs and decreased in the post-operation EVs in each RNA biotype. The levels of 63 mRNAs, 3 lncRNAs, 2 miRNAs and 1 piRNA were significantly increased in the tumors and decreased in the post-operation urinary EVs, thus suggesting that these RNAs mainly originate from PC tissue. No such RNA biomarkers were identified in plasma EVs. This suggests that the fraction of PC-derived EVs in urine is larger than in plasma and allows the detection and tracking of PC-derived RNAs.
Towards a multi-abrasive grinding model for the material point method
Authors: Leroch S. , Grützmacher P. G. , Heckes H. , Eder S. J.
Published: February 2023

An efficient optimization of surface finishing processes can save high amounts of energy and resources. Because of the large occurring deformations, grinding processes are notoriously difficult to model using standard (mesh-based) micro-scale modeling techniques. In this work, we use the meshless material point method to study the influence of abrasive shape, orientation, rake angle, and infeed depth on the grinding result. We discuss the chip morphology, the surface topography, cutting versus plowing mode, the material removal rate, and the chip temperature. A generalization of our model from a straightforward single-abrasive approach to a multiple-abrasive simulation with pseudo-periodical boundary conditions greatly increases the degree of realism and lays the foundation for comparison with real finishing processes. We finally compare our results for multiple abrasives to those obtained for a scaled-down molecular dynamics system and discuss similarities and differences.
Prediction of short-term prognosis of patients with hypertensive intracerebral hemorrhage by radiomic-clinical nomogram
Authors: Wang Jing , Zhou Lu , Chen Yuanyuan , Zhou Hongli , Tan Yuanxin , Zhong Weijia , Zhou Zhiming
Published: February 2023

Hypertensive intracerebral hemorrhage (HICH) is the most common type of spontaneous intracerebral hemorrhage in China which is associated with high mortality and disability. We sought to develop and validate a noncontrast computed tomography (NCCT)-based nomogram model to achieve short-term prognostic prediction for patients with HICH. We retrospectively studied 292 patients with HICH from two medical centers, and they were divided into training (n = 151), validation (n = 66), and testing cohorts (n = 75). Based on radiomics, univariate and multivariate, and logistic regression analyses, four models (black hole sign, clinical, radiomics score, and combined models) were established to predict the prognosis of patients with HICH 30 days after the onset. The results suggested that the combined model had the best predictive performance with the area under the receiver operating characteristic curve (AUC) of 0.821, 0.816, and 0.815 in the training, validation, and testing cohorts, respectively. In addition, a radiomics-clinical (R-C) nomogram was visualized. A calibration curve analysis showed that the R-C nomogram had satisfactory calibration in the three cohorts. A decision curve analysis demonstrated that the R-C nomogram was clinically valuable. Our results suggest that the R-C nomogram can accurately and reliably predict the short-term prognosis of patients with HICH and provide a useful evaluation for making individualized treatment plans.
Phenotyping variants of tumefactive demyelinating lesions according to clinical and radiological features—A case series
Authors: Boyle Thérèse , Fernando Suran L. , Drummond James , Fontes Ariadna , Parratt John
Published: February 2023

Background

Tumefactive demyelinating lesions (TDLs) are defined as lesions >2 cm on MRI of the brain. They are identified in a range of demyelinating diseases including massive demyelination due to Marburg's acute MS, Schilder's Disease, Balo's concentric sclerosis, and Tumefactive MS. Apart from the rare demyelinating variants which are often diagnosed histologically, there are no detailed data to phenotype TDLs.

Methods

We describe the clinical and radiological features of four similar patients with very large TDLs (>4 cm), that are not consistent with the rare demyelinating variants and may represent a distinct phenotype.

Results

All patients presented with hemiplegia and apraxia. The mean age at onset was 37 years with an equal sex distribution. All patients were diagnosed with Tumefactive demyelination based on MRI and CSF analysis, precluding the need for brain biopsy. All responded to potent immunotherapy (including high dose corticosteroids, plasma exchange, rituximab, and/or cyclophosphamide). The mean lag from diagnosis to treatment was 1 day. The median EDSS at presentation was six and recovery to a median EDSS of two occurred over 6 months.

Conclusion

We propose that Tumefactive lesions larger than 4 cm are termed “Giant demyelinating lesions” (GDLs) not only on the basis of size, but a rapid and fulminant demyelinating presentation leading to acute, severe neurological disability that is, nonetheless, responsive to immunotherapy. Further clinical studies are required to ratify this proposed phenotype, establish the immunological profile and best treatment for such patients.
From study abroad to study at home: Spontaneous neuronal activity predicts depressive symptoms in overseas students during the COVID-19 pandemic
Authors: Li Tong , Du Xiaoyu , Zhang Xiang , Dong Aiping , Yuan Xianshun , Yu Tianyi , Diao Ruiyuan , Duan Shuai , Shen Zijian , Yuan Letian , Wang Ximing
Published: February 2023

The objective of this study was to evaluate symptoms of depression and anxiety as well as changes in spontaneous neuronal activity in college students studying abroad during the coronavirus 2019 (COVID-19) pandemic. We examined functional brain changes using resting-state functional magnetic resonance imaging (fMRI), the amplitude of low-frequency fluctuations (ALFF), and regional homogeneity (ReHo) in overseas students with enforced isolation due to the COVID-19 pandemic. Additionally, emotional assessments were administered to determine the severity of depression and anxiety. The questionnaire results showed that anxiety and depressive symptoms differed between overseas students (i.e., those attending an overseas college virtually) and local students (i.e., those attending a local college in person). The fMRI data revealed higher ALFF values in the bilateral superior medial frontal gyrus, bilateral pre-central gyrus, left insula, and left superior temporal gyrus as well as lower ALFF values in the bilateral paracentral lobule (supplementary motor area) in overseas students. Moreover, ReHo analysis also revealed significant differences between overseas students and local students. Compared with local students, overseas students showed significantly increased ReHo in the right inferior frontal and superior temporal gyri and decreased ReHo in the bilateral paracentral lobule, bilateral superior medial frontal gyrus (supplementary motor area), and bilateral pre-central gyrus. In addition, in overseas students, altered ReHo in the cluster including the left superior and medial frontal gyri, pre-central gyrus, and paracentral lobule was significantly positively correlated with Self-Rating Depression Scale scores. Thus, spontaneous brain activity in overseas students changed during the COVID-19 pandemic. This change in brain function might be related to depression and anxiety symptoms. These results suggest that mental health services are needed to decrease the risk of anxiety and depression among college students studying abroad during the COVID-19 pandemic.
Safety and efficacy of a modified busulfan/cyclophosphamide conditioning regimen incorporating cladribine for autologous hematopoietic stem cell transplantation in acute myeloid leukemia
Authors: Shi Yuan-Yuan , Liu Zeng-Yan , Zhang Gui-Xin , He Yi , Han Ming-Zhe , Feng Si-Zhou , Zhang Rong-Li , Jiang Er-Lie
Published: February 2023

This is a small phase I study examining the safety and efficacy of a cladribine (CLAD)-containing conditioning regimen prior to autologous hematopoietic stem cell transplantion (auto-HSCT) for patients with acute myeloid leukemia (AML). All patients, aged 15–54 years (median 32 years), had favorable/intermediate risk AML (n = 20) or acute promyelocytic leukemia (APL; n = 2) and no evidence of minimal residual disease (MRD) prior to transplantation. Fourteen of the 22 patients received the conditioning regimen as follows: busulfan (Bu) + cyclophosphamide (Cy) + CLAD + cytarabine (Ara-c) or idarubicin. The conditioning regimen of 8 patients was without Cy nor idarubicin to reducing adverse cardiac reaction: the regimen of Bu + CLAD+ Ara-c for 6 patients; and the regimen of Bu + melphalan + CLAD + Ara-c for the other 2 patients. All 22 AML patients received peripheral blood stem cell transplantation. The number of infused mononuclear cells and CD34+ cells was 10.00 (2.88–20.97) × 108/kg and 1.89 (1.52–10.44) × 106/kg, respectively. Hematopoietic reconstitution was achieved in all patients, with a median time of 13 (10–34) days for neutrophils and 28 (14–113) days for platelets. Two patients suffered from pulmonary infection, 4 patients suffered from septicemia during the neutropenic stage, and the others suffered from infection or gastrointestinal reaction without exceeding grade 3 after conditioning, which were all alleviated by anti-infection and other supportive treatment. None of the patients died of transplantation-related complications. At a median follow-up of 29.5 (ranging from 4.0 to 60.0) months, three patients relapsed after auto-HSCT at a median time of 6 (ranging from 0.5 to 10.0) months. One patient died due to relapse at 18 months after auto-HSCT. The remaining 21 patients were all alive, including 19 patients with negative MRD. The other 2 patients achieved negative MRD after allogeneic HSCT or chemotherapy. The estimated 2-year survival, relapse, and Leukemia-free survival rates were 94.1 ± 5.7%, 14.7 ± 7.9% and 85.3 ± 7.9%, respectively. A CLAD-combination conditioning regimen is efficient and safe for auto-HSCT, indicating an effective approach for AML treatment.
Preparation and optimization of surface stabilized cryptotanshinone nanocrystals with enhanced bioavailability
Authors: Zhao Wenzheng , Ruan Bohao , Sun Xiaoyi , Yu Zhenwei
Published: February 2023

Cryptotanshinone (CTS) is a plant product extracted from Salvia miltiorrhiza Bunge with various pharmacological significances. In addition to its activities against coronary heart disease, hyperlipidemia, stroke, hepatitis and chronic renal failure, it demonstrates antimetastatic effects. However, its clinical use is limited due to its poor aqueous solubility and oral bioavailability. Herein, CTS nanocrystals were prepared with the precipitation method followed by high-pressure homogenization using Poloxamer 407 as the stabilizer. A stable product was further obtained by lyophilization. The particle size of the CTS nanocrystals was 315.67 ± 11.02 nm, and the zeta potential was near 0 mV. The crystallinity was confirmed by DSC and PXRD. The saturation solubility was substantially increased from 0.97 ± 0.12 μg/ml to 62.29 ± 1.91 μg/ml, and the dissolution rate was also significantly accelerated. A pharmacokinetic study in rats revealed an improvement in oral bioavailability (2.87-fold) with CTS nanocrystals compared to the raw drug. In conclusion, the results of this study suggest a feasible formulation for the oral delivery of CTS.
Daily activity patterns and body temperature of the Oriental migratory locust, Locusta migratoria manilensis (Meyen), in natural habitat
Authors: Li Hongmei , Zhu Jingquan , Cheng Yumeng , Zhuo Fuyan , Liu Yinmin , Huang Jingfeng , Taylor Bryony , Luke Belinda , Wang Meizhi , González-Moreno Pablo
Published: February 2023

Current pest management techniques would benefit from understanding the behavioural rhythms of the target pest and its body temperature, a critical aspect not well studied and potentially limiting the effectiveness of biopesticides under natural conditions. This study aims 1) to understand under natural conditions the behavioural patterns of different stages of hoppers and adults of Locusta migratoria manilensis and 2) to identify the environmental factors modulating their body temperature through field observation. We carried out an intensive field sampling in two of the main locust breeding regions in China, recording the body temperature (day and night), morphological traits (stage, sex and size) and microhabitat of 953 individuals. The results revealed that locusts preferred the ground as their main activity subhabitat, particularly for hoppers. Adults tended to move upper in the reed canopy at two peaks (10-11 h and 14-15 h). Locusts body temperature during daytime increased with development stage and size, while the opposite pattern occurred during night time. Entompathogenic fungi are more effective if the body temperature of the target pest is in a proper range without too high or too low. Application of biopesticides should focus on younger locusts spraying in the morning or at dusk as the locusts have lower body temperatures.
The effect of explicit and implicit online self-compassion interventions on sleep quality among Chinese adults: A longitudinal and diary study
Authors: Sheng Haili , Wang Ruibing , Liu Conghui
Published: February 2023

Objective

This study aimed to investigate the effects of explicit and implicit online intervention methods for self-compassion on improving sleep quality among Chinese adults.

Methods

A total of 328 adult participants were recruited to complete the Pittsburgh Sleep Quality Questionnaire and Self-compassion Scale, and 168 participants were randomly assigned to one of three different conditions: two self-compassion intervention groups (self-compassion writing intervention asked participants to write several sentences with self-compassion, and self-compassion combination intervention asked participants to combine words into complete sentences with self-compassion) and one control group. After 1 week of online self-compassion intervention with daily sleep quality measured simultaneously, 150 participants completed the posttest of the Pittsburgh Sleep Quality Questionnaire and Self-Compassion Scale.

Results

The pretest and posttests results showed that the self-compassion level and sleep quality of the self-compassion writing intervention group were significantly better than those of the control group. However, there was no significant difference between the self-compassion combination intervention group and the control group. For the diary tracking results, sleep quality was significantly better for both the self-compassion writing intervention group and self-compassion combination intervention group than the control group, however, the self-compassion writing intervention group showed great improvements.

Conclusion

Both self-compassion writing and combination interventions were effective in improving sleep quality, and the effect of self-compassion writing was more stable.
Meaningful approaches to content selection and ways of working: Norwegian instrumental music teachers’ experiences
Authors: Jordhus-Lier Anne , Karlsen Sidsel , Nielsen Siw Graabræk
Published: February 2023

The Norwegian municipal schools of music and arts are publicly funded institutions which offer extra-curricular activities for children and adolescents in music and other art forms. The system is designed to ideally reach all children, but while each municipality is legally responsible for providing school of music and arts education for its inhabitants, the law does not state anything about teaching content or ways of working. Consequently, this is up to the teachers to decide. What is taught in these schools is, however, relevant to whether children feel included or excluded. This means that music teachers’ beliefs and actions are among the factors that influence who will feel welcomed and who will feel alienated. On this basis, in this article we explore music teachers’ approach to content-related decision-making processes by asking about their meaningful approaches to selecting content and ways of working within instrumental music teaching. To answer that question, we discuss what kinds of teaching content the teachers choose in general, as well as for beginner and advanced students, the reasons they express for selecting content, which, if any, music they find not to be suitable as teaching content, and how they work with the selected repertoire. We draw on empirical data from a survey among 151 music teachers and an interview study with 11 music teachers. Discussing results from these sources of data in relation to the Nordic and German music Didaktik theories, enables us to address meaningful approaches to selecting content and ways of working. From the analysis, we draw conclusions about what the music teachers experience as meaningful approaches. These can be summarized as (a) the centrality of the students in the process of selecting content; (b) genre versatility, meaning that students should be exposed to a broad range of musical genres and styles; and (c) that students are exposed to the “classical repertoire,” or the standard repertoire within a genre or tradition. In general, what seems to be meaningful for the teachers is working “close to the student’s wishes and preferences,” but in ways that relate to a variety of Didaktik principles.
The impact of the COVID-19 pandemic on undergraduate and postgraduate students: A cross-sectional survey
Authors: Zhu Lu , Zhou Ying , Huang Yiyue , Lei Xinxin , Guo Haoran , Hu Yibo , Wu Songjiang , Lei Li , Guo Aiyuan
Published: February 2023

Background

The COVID-19 pandemic has impacted many facets of life. This study focuses on undergraduate and postgraduate students in China to explore how the pandemic has affected health status, daily life, learning situations, graduation-related situations, and their studies or work planning.

Methods

This study sent online questionnaires to 2,395 participants to investigate the extent to which they were affected by the epidemic in the various aspects mentioned above and to understand what help they tend to get in the face of these effects.

Results

A total of 2,000 valid questionnaires were collected. The physical health of 82.90% of the respondents was affected to varying degrees, with male students, non-medical students, and graduates being more affected than female students, students with medical majors, and non-graduates, respectively. The proportion of students affected by mental health, the total amount of physical exercise, emotional life, and interpersonal communication was 86.35, 88.65, 80.15, and 90.15%, respectively. Compared with medical students and non-graduates, non-medical students and graduates were more affected. In addition, students’ learning and graduation conditions have also been affected to a certain extent: 13.07% of students may not be able to graduate on time, and the proportion of postgraduate students’ graduations affected was higher than that of undergraduate students.

Conclusion

The COVID-19 pandemic has affected the health status of students, their daily lives, learning situations, and so on to varying degrees. We need to pay attention to the issues, provide practical solutions, and provide a basis for better responses to similar epidemics in the future.