bluuebunny/crossref_metadata_2025 huggingface dataset:
Adhesive and Magnetic Properties of Polyvinyl Butyral Composites with Embedded Metallic Nanoparticles
Authors: Terziyan Tatyana V. , Safronov Alexander P. , Beketov Igor V. , Medvedev Anatoly I. , Armas Sergio Fernandez , Kurlyandskaya Galina V.
Published: December 2021

Magnetic metallic nanoparticles (MNPs) of Ni, Ni82Fe18, Ni50Fe50, Ni64Fe36, and Fe were prepared by the technique of the electrical explosion of metal wire. The average size of the MNPs of all types was in the interval of 50 to 100 nm. Magnetic polymeric composites based on polyvinyl butyral with embedded metal MNPs were synthesized and their structural, adhesive, and magnetic properties were comparatively analyzed. The interaction of polyvinyl butyral (supplied as commercial GE cryogenic varnish) with metal MNPs was studied by microcalorimetry. The enthalpy of adhesion was also evaluated. The positive values of the enthalpy of interaction with GE increase in the series Ni82Fe18, Ni64Fe36, Ni50Fe50, and Fe. Interaction of Ni MNPs with GE polymer showed the negative change in the enthalpy. No interfacial adhesion of GE polymer to the surface of Fe and permalloy MNPs in composites was observed. The enthalpy of interaction with GE polymer was close to zero for Ni95Fe5 composite. Structural characterization of the GE/Ni composites with the MNPs with the lowest saturation magnetization confirmed that they tended to be aggregated even for the materials with lowest MNPs concentrations due to magnetic interaction between permalloy MNPs. In the case of GE composites with Ni MNPs, a favorable adhesion of GE polymer to the surface of MNPs was observed.
An Improved Timed Elastic Band (TEB) Algorithm of Autonomous Ground Vehicle (AGV) in Complex Environment
Authors: Wu Jiafeng , Ma Xianghua , Peng Tongrui , Wang Haojie
Published: December 2021

In recent decades, the Timed Elastic Band (TEB) algorithm is widely used for the AGV local path panning because of its convenient and efficiency. However, it may make a local detour when encountering a curve turn and cause excessive energy consumption. To solve this problem, this paper proposed an improved TEB algorithm to make the AGV walk along the wall when turning, which shortens the planning time and saves energy. Experiments were implemented in the Rviz visualization tool platform of the robot operating system (ROS). Simulated experiment results reflect that an amount of 5% reduction in the planning time has been achieved and the velocity curve implies that the operation was relatively smooth. Practical experiment results demonstrate the effectiveness and feasibility of the proposed method that the robots can avoid obstacles smoothly in the unknown static and dynamic obstacle environment.
Generalisation Gap of Keyword Spotters in a Cross-Speaker Low-Resource Scenario
Authors: Lepak Łukasz , Radzikowski Kacper , Nowak Robert , Piczak Karol J.
Published: December 2021

Models for keyword spotting in continuous recordings can significantly improve the experience of navigating vast libraries of audio recordings. In this paper, we describe the development of such a keyword spotting system detecting regions of interest in Polish call centre conversations. Unfortunately, in spite of recent advancements in automatic speech recognition systems, human-level transcription accuracy reported on English benchmarks does not reflect the performance achievable in low-resource languages, such as Polish. Therefore, in this work, we shift our focus from complete speech-to-text conversion to acoustic similarity matching in the hope of reducing the demand for data annotation. As our primary approach, we evaluate Siamese and prototypical neural networks trained on several datasets of English and Polish recordings. While we obtain usable results in English, our models’ performance remains unsatisfactory when applied to Polish speech, both after mono- and cross-lingual training. This performance gap shows that generalisation with limited training resources is a significant obstacle for actual deployments in low-resource languages. As a potential countermeasure, we implement a detector using audio embeddings generated with a generic pre-trained model provided by Google. It has a much more favourable profile when applied in a cross-lingual setup to detect Polish audio patterns. Nevertheless, despite these promising results, its performance on out-of-distribution data are still far from stellar. It would indicate that, in spite of the richness of internal representations created by more generic models, such speech embeddings are not entirely malleable to cross-language transfer.
A MIMO Radar Signal Processing Algorithm for Identifying Chipless RFID Tags
Authors: Su Chen , Zou Chuanyun , Jiao Liangyu , Zhang Qianglin
Published: December 2021

In this paper, the multiple-input, multiple-output (MIMO) radar signal processing algorithm is efficiently employed as an anticollision methodology for the identification of multiple chipless radio-frequency identification (RFID) tags. Tag-identifying methods for conventional chipped RFID tags rely mostly on the processing capabilities of application-specific integrated circuits (ASICs). In cases where more than one chipless tag exists in the same area, traditional methods are not sufficient to successfully read and distinguish the IDs, while the direction of each chipless tag can be obtained by applying MIMO technology to the backscattering signal. In order to read the IDs of the tags, beamforming is used to change the main beam direction of the antenna array and to receive the tag backscattered signal. On this basis, the RCS of the tags can be retrieved, and associated IDs can be identified. In the simulation, two tags with different IDs were placed away from each other. The IDs of the tags were successfully identified using the presented algorithm. The simulation result shows that tags with a distance of 0.88 m in azimuth can be read by a MIMO reader with eight antennas from 3 m away.
A Hollow-Core Photonic-Crystal Fiber-Optic Gyroscope Based on a Parallel Double-Ring Resonator
Authors: Shen Heliang , Chen Kan , Zou Kang , Gong Yijia , Bi Ran , Shu Xiaowu
Published: December 2021

A novel system structure of resonant fiber optical gyroscope using a parallel double hollow-core photonic crystal fiber ring resonator is proposed, which employs the double closed loop and reciprocal modulation–demodulation technique to solve the problem of the length mismatch between rings. This structure can suppress the residual amplitude modulation noise and laser frequency noise, essentially eliminating the influence of the Rayleigh backscattering noise and dramatically reduce the Kerr-effect-induced drift by three orders of magnitude. Thanks to its excellent noise suppression effect, the sensitivity of this novel system can approach the shot-noise-limited theoretical value of 8.94 × 10−7 rad/s assuming the length of the fiber ring resonator is 10 m.
Green Stability Indicating Organic Solvent-Free HPLC Determination of Remdesivir in Substances and Pharmaceutical Dosage Forms
Authors: Ibrahim Adel Ehab , Deeb Sami El , Abdelhalim Emad Mahmoud , Al-Harrasi Ahmed , Sayed Rania Adel
Published: December 2021

A green liquid chromatographic method is considered in this work to minimize the environmental impact of waste solvents. One important principle is to replace or eliminate the use of hazardous organic solvents. Organic impurities in any active pharmaceutical ingredient could arise either during the process of its synthesis, or as degradation products developed throughout the shelf-life. Remdesivir (RDS) is an antiviral drug, approved by the US Food and Drug Adminstration (-FDA), to treat SARS-Cov-2 virus during its pandemic crisis. We studied the stability of remdesivir against several degradation pathways using the organic solvent-free liquid chromatographic technique. Separation was performed on RP-C18 stationary phase using mixed-micellar mobile phase composed of a mixture of 0.025 M Brij-35, 0.1 M sodium lauryl sulfate (SLS), and 0.02 M disodium hydrogen phosphate, adjusted to pH 6.0. The mobile phase flow rate was 1 mL min−1, and detection was carried out at a wavelength of 244 nm. We profiled the impurities that originated in mild to drastic degradation conditions. The method was then validated according to International Conference of Harmonization (ICH) guidelines within a linearity range of 5–100 μg mL−1 and applied successfully for the determination of the drug in its marketed dosage form. A brief comparison was established with reported chromatographic methods, including a greenness assessment on two new metrics (GAPI and AGREE). This study is the first to be reported as eco-friendly, solvent-free, and stability indicating LC methodology for RDS determination and impurity profiling.
Flexible, Biocompatible PET Sheets: A Platform for Attachment, Proliferation and Differentiation of Eukaryotic Cells
Authors: Samanta Soumen , Gaad Diana , Cabet Eva , Lilienbaum Alain , Singh Ajay , Aswal Dinesh K. , Chehimi Mohamed M.
Published: December 2021

Transparent, flexible, biaxially oriented polyethylene terephthalate (PET) sheets were modified by bioactive polymer-fibronectin top layers for the attachment of cells and growth of muscle fibers. Towards this end, PET sheets were grafted with 4-(dimethylamino)phenyl (DMA) groups from the in situ generated diazonium cation precursor. The arylated sheets served as macro-hydrogen donors for benzophenone and the growth of poly(2-hydroxy ethyl methacrylate) (PHEMA) top layer by surface-confined free radical photopolymerization. The PET-PHEMA sheets were further grafted with fibronectin (FBN) through the 1,1-carbonyldiimidazole coupling procedure. The bioactive PET-PHEMA-I-FBN was then employed as a platform for the attachment, proliferation and differentiation of eukaryotic cells which after a few days gave remarkable muscle fibers, of ~120 µm length and ~45 µm thickness. We demonstrate that PET-PHEMA yields a fast growth of cells followed by muscle fibers of excellent levels of differentiation compared to pristine PET or standard microscope glass slides. The positive effect is exacerbated by crosslinking PHEMA chains with ethylene glycol dimethacrylate at initial HEMA/EGDA concentration ratio = 9/1. This works conclusively shows that in situ generated diazonium salts provide aryl layers for the efficient UV-induced grafting of biocompatible coating that beneficially serve as platform for cell attachment and growth of muscle fibers.
Role of Iron Phthalocyanine Coordination in Catecholamines Detection
Authors: Martin Cibely S. , Rubira Rafael J. G. , Silva Jaqueline N. , Aléssio Priscila
Published: December 2021

Catecholamines are an important class of neurotransmitters responsible for regularizing, controlling, and treating neural diseases. Based on control and diseases treatment, the development of methodology and dives to sensing is a promissory technology area. This work evaluated the role of iron phthalocyanine coordination (FePc) with the specific groups from catecholamine molecules (L-dopa, dopamine, epinephrine, and the amino acid tyrosine) and the effect of this coordination on electrochemical behavior. The in situ coordination analysis was performed through isotherms π-A of FePc Langmuir films in the absence and presence of catecholamines. The π-A isotherm indicates a strong interaction between FePc monolayer and L-Dopa and DA, which present a catechol group and a side chain with a protonated amino group (-NH3+). These strong interactions with catechol and amine groups were confirmed by characterization at the molecular level using the surface-enhanced Raman spectroscopy (SERS) from a Langmuir–Schaefer monolayer deposited onto Ag surfaces. The electrochemical measurements present a similar tendency, with lower oxidation potential observed to DA>L-Dopa>Ep. The results corroborate that the coordination of the analyte on the electron mediator surface plays an essential role in an electrochemical sensing application. The FePc LS film was applied as a sensor in tablet drug samples, showing a uniformity of content of 96% for detecting active compounds present in the L-Dopa drug samples.
Cytotoxicity of an Innovative Pressurised Cyclic Solid–Liquid (PCSL) Extract from Artemisia annua
Authors: Culurciello Rosanna , Bosso Andrea , Di Fabio Giovanni , Zarrelli Armando , Arciello Angela , Carella Francesca , Leonardi Leonardo , Pazzaglia Laura , De Vico Gionata , Pizzo Elio
Published: December 2021

Therapeutic treatments with Artemisia annua have a long-established tradition in various diseases due to its antibacterial, antioxidant, antiviral, anti-malaria and anti-cancer effects. However, in relation to the latter, virtually all reports focused on toxic effects of A. annua extracts were obtained mostly through conventional maceration methods. In the present study, an innovative extraction procedure from A. annua, based on pressurised cyclic solid–liquid (PCSL) extraction, resulted in the production of a new phytocomplex with enhanced anti-cancer properties. This extraction procedure generated a pressure gradient due to compressions and following decompressions, allowing to directly perform the extraction without any maceration. The toxic effects of A. annua PCSL extract were tested on different cells, including three cancer cell lines. The results of this study clearly indicate that the exposure of human, murine and canine cancer cells to serial dilutions of PCSL extract resulted in higher toxicity and stronger propensity to induce apoptosis than that detected by subjecting the same cells to Artemisia extracts obtained through canonical extraction by maceration. Collected data suggest that PCSL extract of A. annua could be a promising and economic new therapeutic tool to treat human and animal tumours.
Pain Reduction in Adults with Limb Spasticity Following Treatment with IncobotulinumtoxinA: A Pooled Analysis
Authors: Wissel Jörg , Camões-Barbosa Alexandre , Comes Georg , Althaus Michael , Scheschonka Astrid , Simpson David M.
Published: December 2021

Some studies have shown that incobotulinumtoxinA reduces spasticity-associated pain, but further evidence is needed. This exploratory analysis pooled pain-relief data from six Phase 2 or 3 studies of incobotulinumtoxinA (four placebo-controlled studies) for treating upper limb spasticity in adults. Spasticity-associated pain was assessed at baseline and 4 weeks post incobotulinumtoxinA injection using the disability assessment scale (DAS) for pain. Only data for patients with pain at baseline were analysed. Overall, 544 (incobotulinumtoxinA, N = 415; placebo, N = 129) of 937 patients (58.1%) experienced pain at baseline. At Week 4, a significantly greater proportion of incobotulinumtoxinA- (52.1%) than placebo-treated patients (28.7%; Chi-square p < 0.0001) showed a response (≥1-point improvement in DAS pain score). In logistic regression analysis, incobotulinumtoxinA-treated patients were 2.6 times more likely to achieve this endpoint than placebo-treated patients. A significant difference between incobotulinumtoxinA and placebo was observed regardless of baseline pain severity. Additionally, 27.1% of incobotulinumtoxinA- versus 12.4% of placebo-treated patients reported complete pain relief at Week 4 (p = 0.0006). Pain relief increased with multiple injection cycles. To achieve patient-centred care, pain relief may be considered a treatment goal in adults with spasticity-associated pain regardless of pain severity. This study contributes to understanding the benefits of incobotulinumtoxinA in treating limb spasticity-associated pain.
Triceps Surae Muscle Characteristics in Spastic Hemiparetic Stroke Survivors Treated with Botulinum Toxin Type A: Clinical Implications from Ultrasonographic Evaluation
Authors: Battaglia Marco , Cosenza Lucia , Scotti Lorenza , Bertoni Michele , Polverelli Marco , Loro Alberto , Santamato Andrea , Baricich Alessio
Published: December 2021

Equinovarus foot is one of the most commonly spasticity related conditions in stroke survivors, leading to an impaired gait and poor functional performances. Notably, spastic muscles undergo a dynamic evolution following typical pathophysiological patterns. Botulinum Neurotoxin Type A (BoNT-A) is the gold standard for focal spasticity treatment, and ultrasound (US) imaging is widely recommended to guide injections and monitor muscle evolution. The role of BoNT-A in influencing muscle fibroadipose degeneration is still unclear. In this study, we analyzed medial gastrocnemius (MG) and soleus (SOL) US characteristics (cross-sectional area, muscle thickness, pennation angle, and mean echo intensity) in 53 patients. MG and SOL alterations, compared to the unaffected side, depend on the spasticity only and not on the BoNT-A treatment. In functionally preserved patients (functional ambulation classification, FAC > 3; modified Ashworth scale, MAS ≤ 2), the ultrasonographic changes of MG compared to ipsilateral SOL observed in the paretic limb alone seems to be due to histological, anatomical, pathophysiological, and biomechanical differences between the two muscles. In subjects with poor walking capability and more severe spasticity, such ipsilateral difference was found in both calves. In conclusion, BoNT-A does not seem to influence muscle degeneration. Similar muscles undergo different evolution depending on the grade of walking deficit and spasticity.
Prevalence of Mycotoxins and Endotoxins in Total Mixed Rations and Different Types of Ensiled Forages for Dairy Cows in Lithuania
Authors: Vaičiulienė Gintarė , Bakutis Bronius , Jovaišienė Jurgita , Falkauskas Rimvydas , Gerulis Gediminas , Kerzienė Sigita , Baliukonienė Violeta
Published: December 2021

In this study, 119 samples of total mixed rations and different types of ensiled forage (maize and grass silage, and haylage) collected in 2019–2020 from dairy farms in Lithuania were analyzed to evaluate the quantitative occurrence of mycotoxins and endotoxins. Samples were analyzed using high-performance liquid chromatography (HPLC) with a fluorescent (FLD) and an ultraviolet detector (UV) of mycotoxins and a detection assay based on the ELISA technology for endotoxins. The study included toxins regulated within the European Union (aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON) and T-2 toxin) and nonregulated toxins (endotoxins). Mycotoxin analysis showed that 49.58% of the samples out of 119 were positive for AFB1, 52.11% for ZEA and DON, 55.47% for T-2 toxin and 84.04% for endotoxins. In the contaminated samples, the highest mean values of AFB1 and T-2 toxin were determined in the grass silage samples, while ZEA and DON–were determined in the maize silage samples. Maize silage samples had the highest ZEA and DON concentrations, exceeding the EU maximum permissible concentration limits. In the haylage samples, AFB1 mycotoxin exceeded the maximum concentration limits. The highest mean value of endotoxins was determined in the total mixed rations samples. This is the first study to provide information about the concentrations of mycotoxins and endotoxins in total mixed rations and different types of ensiled forages for dairy cows in Lithuania.
Evidence of the Involvement of a Cyclase Gene in the Biosynthesis of Ochratoxin A in Aspergillus carbonarius
Authors: Ferrara Massimo , Gallo Antonia , Cervini Carla , Gambacorta Lucia , Solfrizzo Michele , Baker Scott E. , Perrone Giancarlo
Published: December 2021

Ochratoxin A (OTA) is a well-known mycotoxin with wide distribution in food and feed. Fungal genome sequencing has great utility for identifying secondary metabolites gene clusters for known and novel compounds. A comparative analysis of the OTA-biosynthetic cluster in A. steynii, A. westerdijkiae, A. niger, A. carbonarius, and P. nordicum has revealed a high synteny in OTA cluster organization in five structural genes (otaA, otaB, ota, otaR1, and otaD). Moreover, a recent detailed comparative genome analysis of Aspergilli OTA producers led to the identification of a cyclase gene, otaY, located in the OTA cluster between the otaA and otaB genes, encoding for a predicted protein with high similarity to SnoaLs domain. These proteins have been shown to catalyze ring closure steps in the biosynthesis of polyketide antibiotics produced in Streptomyces. In the present study, we demonstrated an upregulation of the cyclase gene in A. carbonarius under OTA permissive conditions, consistent with the expression trends of the other OTA cluster genes and their role in OTA biosynthesis by complete gene deletion. Our results pointed out the involvement of a cyclase gene in OTA biosynthetic pathway for the first time. They represent a step forward in the understanding of the molecular basis of OTA biosynthesis in A. carbonarius.
Singularities in Inflationary Cosmological Models
Authors: Fernández-Jambrina Leonardo
Published: December 2021

Due to the accelerated expansion of the universe, the possibilities for the formation of singularities has changed from the classical Big Bang and Big Crunch singularities to include a number of new scenarios. In recent papers it has been shown that such singularities may appear in inflationary cosmological models with a fractional power scalar field potential. In this paper we enlarge the analysis of singularities in scalar field cosmological models by the use of generalised power expansions of their Hubble scalars and their scalar fields in order to describe all possible models leading to a singularity, finding other possible cases. Unless a negative scalar field potential is considered, all singularities are weak and of type IV.
Exercising under COVID-2x: Conceptualizing Future Green Spaces in Australia’s Neighborhoods
Authors: Spennemann Dirk H. R.
Published: December 2021

The COVID-19 pandemic has proven to be a social and economic disruptor on a global scale, severely curtailing people’s ability to travel and engage in many recreation activities. Periodic lockdown and stay-at-home orders have exacerbated the situation. In this social climate, urban green spaces have attained a high significance for the maintenance of the physical and mental health of the population. Given the presence of similar coronaviruses in animal populations, it can be predicted that future epidemics and even pandemics will occur. Urban planning needs to incorporate the lessons learnt during COVID-19 in order to future-proof our communities. This paper reviews the role that urban green spaces played during the COVID-19 pandemic. Based on these observations, the paper discusses a range of options for the provision of pandemic-sensitive spaces for physical exercise and mental recreation. Design concepts for long-term planning adjustments as well as for future ad-hoc solutions are provided. These include the provision of social distancing ‘pods’ embedded in design and landscaping of permanent parks, the design of ad-hoc, socially distanced ‘parklets’ on a quietened street and a rethink of the design of curb-to-boundary setbacks (nature strip) in residential streets.
Pathological Features of Echovirus-11-Associated Brain Damage in Mice Based on RNA-Seq Analysis
Authors: Zhang Guoyan , Li Jichen , Sun Qiang , Zhang Keyi , Xu Wenbo , Zhang Yong , Wu Guizhen
Published: December 2021

Echovirus 11 (E11) is a neurotropic virus that occasionally causes fatal neurological diseases in infected children. However, the molecular mechanism underlying the disease and pathological spectrum of E11 infection remains unclear. Therefore, we modelled E11 infection in 2-day-old type I interferon receptor knockout (IFNAR−/−) mice, which are susceptible to enteroviruses, with E11, and identified symptoms consistent with the clinical signs observed in human cases. All organs of infected suckling mice were found to show viral replication and pathological changes; the muscle tissue showed the highest viral replication, whereas the brain and muscle tissues showed the most obvious pathological changes. Brain tissues showed oedema and a large number of dead nerve cells; RNA-Seq analysis of the brain and hindlimb muscle tissues revealed differentially expressed genes to be abundantly enriched in immune response-related pathways, with changes in the Guanylate-binding protein (GBP) and MHC class genes, causing aseptic meningitis-related symptoms. Furthermore, human glioma U251 cell was identified as sensitive target cells for E11 infection. Overall, these results provide new insights into the pathogenesis and progress of aseptic meningitis caused by E11.
Immunopathology of RSV: An Updated Review
Authors: Bergeron Harrison C. , Tripp Ralph A.
Published: December 2021

RSV is a leading cause of respiratory tract disease in infants and the elderly. RSV has limited therapeutic interventions and no FDA-approved vaccine. Gaps in our understanding of virus–host interactions and immunity contribute to the lack of biological countermeasures. This review updates the current understanding of RSV immunity and immunopathology with a focus on interferon responses, animal modeling, and correlates of protection.
Production- and Purification-Relevant Properties of Human and Murine Cytomegalovirus
Authors: Ravlić Sanda , Brgles Marija , Hiršl Lea , Jonjić Stipan , Halassy Beata
Published: December 2021

There is a large unmet need for a prophylactic vaccine against human cytomegalovirus (HCMV) to combat the ubiquitous infection that is ongoing with this pathogen. A vaccination against HCMV could protect immunocompromised patients and prevent birth defects caused by congenital HCMV infections. Moreover, cytomegalovirus (CMV) has a number of features that make it a very interesting vector platform for gene therapy. In both cases, preparation of a highly purified virus is a prerequisite for safe and effective application. Murine CMV (MCMV) is by far the most studied model for HCMV infections with regard to the principles that govern the immune surveillance of CMVs. Knowledge transfer from MCMV and mice to HCMV and humans could be facilitated by better understanding and characterization of the biological and biophysical properties of both viruses. We carried out a detailed investigation of HCMV and MCMV growth kinetics as well as stability under the influence of clarification and different storage conditions. Further, we investigated the possibilities to concentrate and purify both viruses by ultracentrifugation and ion-exchange chromatography. Defective enveloped particles were not separately analyzed; however, the behavior of exosomes was examined during all experiments. The effectiveness of procedures was monitored using CCID50 assay, Nanoparticle tracking analysis, ELISA for host cell proteins, and quantitative PCR for host cell DNA. MCMV generally proved to be more robust in handling. Despite its greater sensitivity, HCMV was efficiently (100% recovery) purified and concentrated by anion-exchange chromatography using QA monolithic support. The majority of the host genomic DNA as well as most of the host cell proteins were removed by this procedure.
A Novel Terrestrial Rabies Virus Lineage Occurring in South America: Origin, Diversification, and Evidence of Contact between Wild and Domestic Cycles
Authors: Caraballo Diego A. , Lema Cristina , Novaro Laura , Gury-Dohmen Federico , Russo Susana , Beltrán Fernando J. , Palacios Gustavo , Cisterna Daniel M.
Published: December 2021

The rabies virus (RABV) is characterized by a history dominated by host shifts within and among bats and carnivores. One of the main outcomes of long-term RABV maintenance in dogs was the establishment of variants in a wide variety of mesocarnivores. In this study, we present the most comprehensive phylogenetic and phylogeographic analysis, contributing to a better understanding of the origins, diversification, and the role of different host species in the evolution and diffusion of a dog-related variant endemic of South America. A total of 237 complete Nucleoprotein gene sequences were studied, corresponding to wild and domestic species, performing selection analyses, ancestral states reconstructions, and recombination analyses. This variant originated in Brazil and disseminated through Argentina and Paraguay, where a previously unknown lineage was found. A single host shift was identified in the phylogeny, from dog to the crab-eating fox (Cerdocyon thous) in the Northeast of Brazil. Although this process occurred in a background of purifying selection, there is evidence of adaptive evolution -or selection of sub-consensus sequences- in internal branches after the host shift. The interaction of domestic and wild cycles persisted after host switching, as revealed by spillover and putative recombination events.
Cardiomyopathy Associated with Anti-SARS-CoV-2 Vaccination: What Do We Know?
Authors: Parra-Lucares Alfredo , Toro Luis , Weitz-Muñoz Sebastián , Ramos Cristóbal
Published: December 2021

The SARS-CoV-2 pandemic has mobilized many efforts worldwide to curb its impact on morbidity and mortality. Vaccination of the general population has resulted in the administration of more than 6,700,000,000 doses by the end of October 2021, which is the most effective method to prevent hospitalization and death. Among the adverse effects described, myocarditis and pericarditis are low-frequency events (less than 10 per 100,000 people), mainly observed with messenger RNA vaccines. The mechanisms responsible for these effects have not been specified, considering an exacerbated and uncontrolled immune response and an autoimmune response against specific cardiomyocyte proteins. This greater immunogenicity and reactogenicity is clinically manifested in a differential manner in pediatric patients, adults, and the elderly, determining specific characteristics of its presentation for each age group. It generally develops as a condition of mild to moderate severity, whose symptoms and imaging findings are self-limited, resolving favorably in days to weeks and, exceptionally, reporting deaths associated with this complication. The short- and medium-term prognosis is favorable, highlighting the lack of data on long-term evolution, which should be determined in longer follow-ups.
COVID-19 Immunisation, Willingness to Be Vaccinated and Vaccination Strategies to Improve Vaccine Uptake in Australia
Authors: Wang Bing , Nolan Rebecca , Marshall Helen
Published: December 2021

The COVID-19 vaccine rollout is crucial to lifting community and economic restrictions. This cross-sectional study aimed to assess: (a) COVID-19 vaccine uptake and associated factors; (b) COVID-19 vaccine intentions and associated factors; (c) community support for COVID-19 vaccination strategies and associated factors. The survey was conducted between May and July 2021 in Australia. Of 3003 participants, 30% reported they were already vaccinated and 39% indicated they would get vaccinated. Low socioeconomic and education levels, non-English speaking backgrounds and being parents were associated with decreased vaccine willingness and/or vaccination rates. High levels of support for vaccination strategies were demonstrated with mandatory vaccination being less preferable. Respondents from non-English speaking backgrounds were more likely to support a mandatory vaccination policy. Respondents with the highest socioeconomic level were more likely to support vaccination requirements for international travel, visiting nursing homes and working in healthcare settings. Respondents who were aged ≥70 years were more likely to support all proposed vaccination strategies. Targeted campaigns should be implemented for parents and those who live in socioeconomic disadvantaged areas and have lower educational attainment. Concise and clear vaccine information should be provided in lay and multiple languages to improve vaccine confidence. Vaccine enforcement policies should be considered and implemented with caution.
Relics and Historical Uses of Human Zootherapeutic Products in Contemporary Spanish Ethnoveterinary Medicine
Authors: González José A. , Vallejo José Ramón
Published: December 2021

(1) Background: this review documents the wide repertoire of practices and remedies based on the use of human-derived products in Spanish ethnoveterinary medicine (EVM) from the early 20th century to the present. These practices are compared with historical data and those of other countries; (2) Methods: a search using advanced functions in the most important databases in the fields of ethnobiology, EVM, folklore, and ethnography was performed. Information was obtained from 29 documentary sources; (3) Results: from the search of the literature, 46 use-reports related to the veterinary use of human urine, menstrual fluid, saliva, breast milk, and faeces were recorded. These zootherapeutic resources are/were used to treat 20 animal diseases, in particular dermatological ailments. In addition, many practices of the magical–religious type are documented; (4) Conclusions: the veterinary uses described and analysed here are fundamental to the development of therapeutic tools and creating teaching and learning processes in new popular veterinary practices adapted to the users and those who demand them. The information collected could form a scientific foundation for future inventories of local veterinary knowledge (LVK) and research addressing the discovery of new drugs for livestock. This work contributes to the inventory of some uses, traditional practices, and rituals seriously threatened by the progressive loss of LVK in Europe.
Helminth Infections in Cattle and Goats in Kanchanaburi, Thailand, with Focus on Strongyle Nematode Infections
Authors: Income Nicharee , Tongshoob Jarinee , Taksinoros Sarawut , Adisakwattana Poom , Rotejanaprasert Chawarat , Maneekan Pannamas , Kosoltanapiwat Nathamon
Published: December 2021

Gastrointestinal helminths are major enteric parasites affecting the health of important livestock ruminants, such as cattle and goats. It is important to routinely survey these animals for helminth infections to allow effective management and control programs to be implemented. A cross-sectional helminth survey carried out in Kanchanaburi Province, Thailand, revealed the infection rate of gastrointestinal helminths in cattle (n = 157) and goats (n = 117) to be 35.7% and 88%, respectively, by microscopic fecal examination, and a 100% herd prevalence was observed in goats. Eggs of strongyle nematodes, Strongyloides spp., Trichuris spp., Capillaria spp., Paramphistomum spp., and Moniezia spp. were detected, with a relatively high rate of strongyle nematode infection in both cattle (28.7%) and goats (86.3%). Mixed infections were observed in 14.3% and 35.9% of egg-positive samples from cattle and goats, respectively. Risk factor analysis showed that dairy cattle were 5.1 times more likely to be infected with strongyles than meat cattle. In contrast, meat goats were 9.3 times more likely to be infected with strongyles than dairy goats. The inverse findings in cattle and goats are discussed. Female gender was associated with a higher risk of strongyle infection in goats. DNA sequencing and in-house semi-nested PCR with primers specific to a region in the internal transcribed spacer 2 (ITS2) were successfully used to identify strongyle genera in randomly selected egg-positive cattle (n = 24) and goat (n = 24) samples. Four strongyle genera, i.e., Cooperia spp., Haemonchus spp., Oesophagostomum spp., and Trichostrongylus spp. were identified by DNA sequencing. By semi-nested PCR, Cooperia spp. were detected as a major parasite of cattle (70.8%), whereas Haemonchus spp. were abundant in goats (100%). The majority of samples from cattle (58.3%) and goats (95.8%) were found to coinfect with at least two strongyle genera, suggesting that coinfection with multiple strongyle genera was more common than single infection in these animals.
Application of Ostrom’s Institutional Analysis and Development Framework in River Water Conservation in Southern Ecuador. Case Study—The Zamora River
Authors: Oñate-Valdivieso Fernando , Massa-Sánchez Priscilla , León Patricio , Oñate-Paladines Arianna , Cisneros Mónica
Published: December 2021

Water is essential for life and human activities. In addition to the constant increase in water demand, there are problems caused by inefficient governance, such as the discharge of untreated wastewater into rivers and seas, which is aggravated by the limited participation of civil society in decision-making. To face current and future challenges, solid public policies must be implemented, focused on measurable objectives, following planned and predetermined schedules on an appropriate scale, based on a clear assignment of functions to the competent authorities, and subject to periodic monitoring and evaluation. The Institutional Analysis and Development framework proposed by Ostrom made it possible to identify gaps in the existing governance, and to establish actions that could strengthen the institutional framework with the active participation of social actors, in order to achieve an effective conservation of water resources in southern Ecuador. The present study determined that regulations are not coherent with the conflict, the design of policies, and the effects of decision-making. The formal rules for wastewater management are not applied, and there is an incipient citizen participation, as well as disarticulation in institutions responsible for wastewater management. Recommendations were made to strengthen the institutional framework and governance of wastewater.
Responses of Net Anthropogenic N Inputs and Export Fluxes in the Megacity of Chengdu, China
Authors: Ding Yao , Lai Chengyue , Shi Qing , Ouyang Lili , Wang Zhaoli , Yao Gang , Jia Binyang
Published: December 2021

Anthropogenic N inputs have become progressively more problematic and have profoundly affected the water quality in megacities throughout China. Thus, to design and implement appropriate megalopolis watershed management, it is important to understand the relationship between N inputs and exports and to identify the N pollution sources. To that end, in this work, the net anthropogenic N inputs (NANI) in Chengdu City were estimated based on statistical data collected between 1970 and 2019. N input fluxes and pollution sources were estimated through sample collection and field measurements that were performed between 2017 and 2019, while nitrate (NO3−) was identified using stable isotope and Bayesian model (SIAR) analysis. The NANI was found to be affected primarily by livestock and poultry consumption of N rich feed. Moreover, the N export fluxes and runoff showed a high degree of correlation. Notably, NO3− fluxes exhibited a significant increase over the course of the study period, such that, by 2019, the total N fluxes (18,883.85 N kg/km2) exceeded the NANI (17,093.87 N kg/km2). The results indicate that although livestock and poultry farming were the original primary sources of NANI, their contributions declined on an annual basis. Moreover, with the emphasis placed on point source management in Chengdu City, domestic sewage discharge has been significantly reduced. Therefore, N retention in groundwater is thought to be the factor driving the N flux increase. These findings are pivotal to solving the N pollution problem in megacities like Chengdu (China).
Machine Learning and Urban Drainage Systems: State-of-the-Art Review
Authors: Kwon Soon Ho , Kim Joong Hoon
Published: December 2021

In the last decade, machine learning (ML) technology has been transforming daily lives, industries, and various scientific/engineering disciplines. In particular, ML technology has resulted in significant progress in neural network models; these enable the automatic computation of problem-relevant features and rapid capture of highly complex data distributions. We believe that ML approaches can address several significant new and/or old challenges in urban drainage systems (UDSs). This review paper provides a state-of-the-art review of ML-based UDS modeling/application based on three categories: (1) operation (real-time operation control), (2) management (flood-inundation prediction) and (3) maintenance (pipe defect detection). The review reveals that ML is utilized extensively in UDSs to advance model performance and efficiency, extract complex data distribution patterns, and obtain scientific/engineering insights. Additionally, some potential issues and future directions are recommended for three research topics defined in this study to extend UDS modeling/applications based on ML technology. Furthermore, it is suggested that ML technology can promote developments in UDSs. The new paradigm of ML-based UDS modeling/applications summarized here is in its early stages and should be considered in future studies.
Molecular Methods for Pathogenic Bacteria Detection and Recent Advances in Wastewater Analysis
Authors: Zhang Shuxin , Li Xuan , Wu Jiangping , Coin Lachlan , O’Brien Jake , Hai Faisal , Jiang Guangming
Published: December 2021

With increasing concerns about public health and the development of molecular techniques, new detection tools and the combination of existing approaches have increased the abilities of pathogenic bacteria monitoring by exploring new biomarkers, increasing the sensitivity and accuracy of detection, quantification, and analyzing various genes such as functional genes and antimicrobial resistance genes (ARG). Molecular methods are gradually emerging as the most popular detection approach for pathogens, in addition to the conventional culture-based plate enumeration methods. The analysis of pathogens in wastewater and the back-estimation of infections in the community, also known as wastewater-based epidemiology (WBE), is an emerging methodology and has a great potential to supplement current surveillance systems for the monitoring of infectious diseases and the early warning of outbreaks. However, as a complex matrix, wastewater largely challenges the analytical performance of molecular methods. This review synthesized the literature of typical pathogenic bacteria in wastewater, types of biomarkers, molecular methods for bacterial analysis, and their recent advances in wastewater analysis. The advantages and limitation of these molecular methods were evaluated, and their prospects in WBE were discussed to provide insight for future development.
A Numerical Simulation on the Leakage Event of a High-Pressure Hydrogen Dispenser
Authors: Wang Benjin , Shen Yahao , Lv Hong , He Pengfei
Published: December 2021

For the sake of the increasing demand of hydrogen fuel cell vehicles, there are more concerns on the safety of hydrogen refueling stations. As one of the key pieces of equipment, the hydrogen dispenser has drawn attention on this aspect since it involves massive manual operations and may be bothered by a high probability of failure. In this paper, a numerical study is conducted to simulate the possible leakage events of the hydrogen dispenser based on a prototype in China whose working pressure is 70 MPa. The leakage accident is analyzed with respect to leakage sizes, leak directions, and the time to stop the leakage. It is found that, due to the large mass flow rate under such high pressure, the leak direction and the layout of the components inside the dispenser become insignificant, and the ignitable clouds will form inside the dispenser in less than 1 s if there is a leakage of 1% size of the main tube. The ignitable clouds will form near the vent holes outside the dispenser, which may dissipate quickly if the leakage is stopped. On the other hand, the gas inside the dispenser will remain ignitable for a long time, which asks for a design with no possible ignition source inside. The results can be useful in optimizing the design of the dispenser, regarding the reaction time and sensitivity requirements of the leakage detector, the size and amount of vent holes, etc.
Gastromania on italian tv
Authors: Giannitrapani Alice
Published: December 2021

By turning on the TV, at any time of the day or night, one can comeacross programs in which food is the undisputed protagonist. Actually, thepresence of food on TV is not a contemporary phenomenon, but it goesback to the origins of television. Over time, the way of narrating food hasbeen transformed, as well as the role attributed to it and the values (gastronomicand social) associated to it.In this paper, after having traced a historical overview of Italian foodtelevision programming, we focus on the analysis of four recent programs.The objective is to understand how settings, rhythmic and temporal scansion,and the distribution of roles among the various actors involved configurenarratives with well-structured mechanisms and convey differentways of understanding cooking, the role of the chef, and the relationshipwith the audience at home.
Estimulación del pensamiento lógico-matemático de los escolares a través de los rincones pedagógicos
Authors: Palma Sánchez Betsy Jazmín , Sabando Intriago Katiuska Maricela , Mestre Gómez Ulises , Delgado Gozembach Janeth De Lourdes
Published: December 2021

Es tendencia actual en la educación el desarrollo de destrezas y habilidades para contribuir y promover en el niño y niña un conocimiento, transformarlo y relacionarlo en crear las actividades de manera eficiente y efectivo en contribuir en un cambio y responder a las necesidades elementales que permitan ayudar al infante en construir aprendizajes significativos y útiles para el desempeño real del desarrollo de las personas en busca de alternativas que enfrentar las consecuencias negativas que sufre en contexto social, cultural y medioambiental. Es importante proporcionar a los niños una gran variedad de lenguajes para la comunicación, aparte del lenguaje hablado. Todos los lenguajes que conviven ya en la mente y en las actividades de los niños deben potenciarse para que crezcan y se conviertan en fuerzas generadoras de otros lenguajes y de otras potencialidades creadoras. En el trabajo se fundamenta la manera en que los espacios didácticos de construcción permiten el desarrollo del pensamiento lógico-matemático en los escolares.
PALABRAS CLAVE: desarrollo habilidades; lenguaje de comunicación; rincones pedagógicos; pensamiento lógico-matemático.
Stimulation of the logical-mathematical thinking of school students through pedagogical corners
ABSTRACT
The current trend in education is the development of skills and abilities to contribute and promote knowledge in children, transform it and relate it to create activities efficiently and effectively to contribute to a change and respond to the basic needs that allow helping the infant in building meaningful and useful learning for the real performance of the development of people in search of alternatives to face the negative consequences suffered in social, cultural and environmental context. It is important to provide children with a wide variety of languages ​​for communication in addition to spoken language. All languages ​​that already coexist in children's minds and activities must be empowered so that they grow and become generating forces for other languages ​​and other creative potentialities. The work is based on the way in which the didactic construction spaces allow the development of logical-mathematical thinking in schoolchildren.
KEYWORDS: skills development; communication language; pedagogical corners; logical-mathematical thinking.