bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparisons of lymphocytes profiles and inflammatory cytokines levels in blood of patients with differed severity of infection by human adenovirus type 7 Running title: HAdV-7 infection and immune dysfunction
Authors: Sun Junping , Ma Xidong , Zhang Mingyue , Xie Mei , Zhang Xingang , Han Xinjie , Li Xinfu , Zhou Enlu , Wang Junyu , She Danyang , Wang Jianxin
Published: September 2022

Background
Human adenovirus (HAdV) infection outbreak causes community-acquired pneumonia. Cellular immune dysfunction and hypercytokinemia play important roles in the pathogenesis of adenovirus respiratory infection. Some soluble factors in peripheral blood can predict the virus-induced disease progression accurately. The expression levels of inflammatory cytokines differ among patients with different disease severity. However, whether and how HAdV-7 infection influences the composition of blood immune cells and serum cytokine levels in patients at different disease stages, as well as the diagnosis and prognosis values of these parameters, have rarely been intensively studied. We aimed to investigate lymphocytes profiles and cytokines levels in blood of patients at different disease stages upon human adenovirus type 7 (HAdV-7) infections, and explored the diagnosis and prognosis values of the investigated parameters.
Methods
Patients from two outbreaks of HAdV-7 in military of China were categorized into upper respiratory infection (URI) group, common pneumonia (CP) group and severe pneumonia (SP) group according to disease severity. Peripheral blood samples were subjected to routine laboratory tests, while flow cytometry and ELISA were used to measure the lymphocyte subsets and cytokines in blood, respectively. The receiver operating characteristic (ROC) curves were performed to examine the diagnostic and prognostic abilities of these blood parameters.
Results
Signs of imbalanced lymphocytes composition and hypercytokinemia were observed in HAdV-7-infected patients. The percentages of CD3+ T cells and NK cells were significantly decreased along with the aggravation of the disease, particularly for NK cells and CD4+ T cells. The neutrophil to lymphocyte ratio (NLR) increased significantly in patients with more severe disease. In addition, the levels of serum CXCL10, IL-2 and TNF-α were positively correlated with disease severity, while reduced levels of IFN-γ and IL-10 were found in SP patients. Furthermore, analysis of ROC showed that multiple parameters including the percentage of blood CD3+ cells and serum CXCL10 level could predict the progression of HAdV-7 infection.
Conclusions
Imbalance of immune state with hypercytokinemia occurred during HAdV-7 infection. The percentages of blood immune cells such as CD3+ T cells and the levels of serum cytokines such as CXCL10 showed potential diagnosis and prognosis values in HAdV-7 infection.
Sediment vibration characteristics based on accelerometer measurements
Authors: Wang Pingyi , Yuan Jun , Wang Meili , Wang Mi
Published: September 2022

The study of the vibration phenomenon of pebbles under turbulence is still a gap despite recent technological advancements in measurement capabilities. In this study, the vibration process of a fully exposed, isolated smart pebble on a rough bed was measured using a miniature inertial accelerometer and combined with simultaneous local measurements of the near-bed velocities. The paper conducts a series of experimental studies with different flow conditions. The test data match well with the manually observed phenomena, indicating the authenticity of the sediment vibration data collected by the measurement system. The test results show that the pebble motion (before entrainment) subjected to turbulence is a nonlinear vibration process, and its vibration types include in-situ vibration and ex-situ strong vibration. The probability distribution for the amplitude of vibration acceleration is well parameterized by the normal distribution. The vibration intensity tends to increase before approaching the threshold , but it weakens when approaching the point. The sediment vibration frequency is within 20~25 Hz, similar to the flow pulsation frequency and belongs to low-frequency vibration. The data indicate that the near-bed flow velocity is most directly related to the particle vibration events.
Hope, coping strategies, and their predictors in older prostate and breast cancer patients under radiotherapy: a cross sectional study
Authors: Yildizel Sema , Arayici Mehmet Emin , Umay Cenk , Sahin Sevnaz
Published: September 2022

Purpose: The aim of this paper was to evaluate the level of hope, coping methods, and the factors affecting them in patients aged 60 years and over who were diagnosed with breast and prostate cancer, who underwent surgery and/or chemotherapy according to the condition of the disease, and who were at the stage of radiotherapy (RT).
Method: A total of 66 patients, 37 breast cancer and 29 prostate cancer patients, were included in this cross-sectional study. RT and treatment information of the patients were collected by using the records in the hospital database. The personal information of the participants was also collected through the personal information form and scales [HHI (Heart Hope Index), COPE (Coping Attitudes Assessment Scale), VAS (Visual Analogue Scale)].
Results:The median age of the participants was 65 (min-max: 60-80) years. There was a decrease in the weight and waist circumference of the participants after RT (p<0.05). There was no significant change in the HHI and COPE total scores of the patients before and after RT (p>0.05). When only patients with prostate cancer were studied, the self-help sub-dimension of COPE was found to be lower (p=0.018).
Conclusions: It is recommended that patients with prostate cancer be evaluated more sensitively in terms of coping levels during and after radiotherapy. There is an increasing need for further studies in larger populations and cohorts related to this topic in the literature.
Implications for Cancer Survivors: Multidimensional evaluations and interventions may be required in terms of hope levels and coping attitudes of cancer patients receiving radiotherapy.
Bifidobacterium animalis sup F1-7 and Lactobacillus plantarum FWDG alleviate the malnutrition of mice via ghrelin-GHSR/PKC pathway
Authors: Lu Youyou , Wang Ruiqi , Wu Yeting , Cui Qingyu , Tian Xiaoying , Zhang Zhe , Yi Huaxi , Liu Tongjie , Gong Pimin , Zhang Lanwei
Published: September 2022

Purpose: Intestinal microecology was closely
related to malnutrition, but the related mechanism was still
unclear. This study aimed to reveal how microorganisms alleviated
malnutrition via ghrelin-GHSR/PKC-SCFAs
pathway.

Methods and results: Melanoma cells
B16F10-induced malnourished mice of lung cancer. Strains Bif.
animalis F1-7 and L. plantarum FWDG alleviated the
malnutrition of mice to some extent by increasing the dietary
intake level of mice, but the effect of Bif. animalis F1-7
was more prominent. This process was through increasing the
expression level of GHSR and PKC, reducing the expression level of
IP3, and finally regulating the secretion level of ghrelin and
improving the anorexia of mice. Meanwhile, Bif. animalis
F1-7 also reduced the expression levels of GLUT2, SGLT1 and PEPT1
genes and proteins, promoted the increase of acetic acid, butyric
acid, isovaleric acid and total acid levels, regulated the
nutritional transport process of intestinal energy, and finally
improved the nutritional status of tumor mice via
ghrelin-GHSR/PKC-SCFAs pathway.

Conclusion: Our study provided a data support
for the application of potentially beneficial microorganisms of
Bif.animalis F1-7 could acts as an auxiliary component to
alleviate malnutrition.
Analysis of communal Pathogenesis and Immune Infiltration Characteristics Between Psoriasis and Pulmonary Arterial Hypertension
Authors: xia qingyue , su wenxing , cheng yuxin , zeng ni , lu zhiyu , Su Wenxing , Luo Dan
Published: September 2022

Background
Increasingly evidence has shown pulmonary arterial hypertension (PAH) was predisposed to occur in psoriasis, however, the common mechanism of this phenomenon is still not fully clarified. This study aims to further explore the molecular mechanisms of this complication.
Methods
Four datasets were downloaded from the Gene Expression Omnibus (GEO) database based on the study inclusion/exclusion criteria. After screening the communal DEGs, modules, and hub genes of psoriasis and PAH, subsequent bioinformatic analyses, consisting of function annotation analysis, co-expression analysis, drug-gene interaction prediction, and mRNA–miRNA regulation network construction were conducted. Moreover, Immune cell infiltration analysis and correlation analysis were performed to further uncover the related immune pathogenesis in psoriasis and PAH.
Results
170 communal DEGs, 4 modules, and 6 hub genes were identified between GSE15197 and GSE30999, and the expression of hub genes was verified in the GSE41662 and GSE113439 respectively. The function annotation analysis of these genes mainly enriched in the Immune System and associated signal transduction, and the immune cell infiltration analysis highlighted the existence of the overlap in terms of mast cells between PAH and psoriasis.
Conclusions
The analysis of communal DEGs, modules, and hub genes underlined the potential role of the immune system and associated signal transduction in the common pathogenesis of psoriasis and PAH, and immune Infiltration analysis of two diseases provide us with new perspectives and exploring direction. Moreover, six hub genes (MYO5A, CDT1, ASPM, ACTR2, PTPN11, and SOST) may be used as biomarkers or therapeutic targets in psoriasis and PAH.
Comparison of clinical and radiological outcomes after three different surgical treatments for resistant calcifying tendinitis of the shoulder: a short-term randomized controlled trial.
Authors: Verstraelen Freek , Schotanus Martijn , Klemann-Harings Steffie , Heerspink Okke Lambers , Jansen Edwin
Published: September 2022

Background: A preferable surgical treatment for patients with conservative therapy resistant calcifying tendinitis of the shoulder is still a matter of debate. Therefore, the purpose of this study was to evaluate and compare short-term clinical and radiological results of three surgical treatment options for these patients.
Methods: A multicenter randomized trial was conducted. 69 patients were randomly assigned to receive 1. subacromial decompression (Group SAD), 2. debridement of calcifications (Group D), or 3. debridement of calcifications with SAD (Group D+SAD). Stringent inclusion and exclusion criteria were used. Primary outcome was improvement in VAS for pain (pVAS) six months postoperatively. Secondary outcomes were improvement in pVAS six weeks postoperatively, functional outcome (CMS, DASH, ASES), radiological outcome, side treatments and complications.
Results: The improvement in pVAS was significant in all groups (p<0.001) and did not differ between the groups after six months. Six weeks postoperatively, the improvement in pVAS was significantly (p=0.03) less in Group SAD compared to Group D+SAD (16.5mm, SD 19.3mm vs 33.1mm, SD 19.7mm, respectively). The mean size of calcifications decreased significantly in all groups (p<0.0001). In Group SAD the size of the calcifications decreased less (p=0.04) compared to Group D and group D+SAD after six weeks. Group SAD received more side treatments (p=0.003) compared to Group D+SAD (9 vs 1), which were mainly subacromial cortisone injections.
Conclusions: All patient groups showed significant pain relief and improvement of shoulder function six months after surgery. However, patients in group SAD showed inferior pain relief and improvement in DASH score after six weeks. Furthermore, this group required more postoperative side treatments. No significant differences in clinical and radiological outcomes were observed between patients in group D compared to group D+SAD. Therefore, an arthroscopic debridement without subacromial decompression seems to be advisable for patients with therapy resistant calcifying tendinitis of the shoulder.
Level of evidence: 2, Open Label Randomized Clinical Trial.
IRB: METC Zuyderland MC. Number: 14-T-112.
Registered at trialregister.nl: NL 4947
Discovery and significance of nanoparticles released by plants
Authors: Zhang Wanqin , Cao JianJin , Luo Xiaoen , Qiu Junwei , Qi Yonghao
Published: September 2022

As an important part of nature, the role of plants in the material cycle has been widely studied. Nanoparticles, as a common form of existence, have also been confirmed to be absorbed and retained by plants, but there are few studies on the environmental effects of exhaled nanoparticles from plants. In this paper, a sampling device is designed to sample the exhaled substances from plants in Fogang, Qingyuan, Guangdong province and Liushutang, Hunan Province, and analyzed by transmission electron microscopy. It is found that plants emit a large number of nanoparticles. The particle diameter is mostly between 50-300nm, usually sulfate and phosphate. The discovery of nanoparticles emitted by plants makes an important supplement to the respiration of plants. It is estimated that about 5.066×1019 nanoparticles are produced by plants every month. These particles have a similar effect to anthropogenic sulfate particles but have a smaller particle size and have an important impact on global cooling and rainfall.
The abnormal glucose metabolism due to endogenous serine synthesis for selenoprotein expression in vitro under high-Se
Authors: Wang Qin , Wang Jianrong , Zhan Shuo , Han Feng , Liu Yiqun , Huang Zhenwu
Published: September 2022

Objective: The purpose of this study is to observe whether the glycolysis bypass is activated under high-Se stress.
Method: Firstly, five different concentrations of selenomethionine (SeMet, 0.001, 0.01, 0.1, 1 and 10 mmol/L) were added into the three kinds of cell lines (L02, HepG2 and HCT-116) and incubated for 48 hours. The expressions of selenoproteins (GPX1 and SELENOP) and key enzymes for the de novo serine biosynthesis (PHGDH) or synthetic serine as the one carbon unit donor (SHMT1) were analyzed by ELISA and WB to screen the most sensitive cell line. Then, with more detailed levels of SeMet (0.001, 0.005, 0.01, 0.025, 0.05, 0.075, 0.1, 0.25, 0.5, 1, and 10 µmol/L) given, L02 cell was cultured for 48 hours to analyze the trends of selenoproteins and serine-related metabolic enzymes by WB. Finally, the impact of exogenous serine or glycine on the expressions of selenoproteins and metabolic enzymes were evaluated in L02 cell cultured in high-Se media.
Result: Unlike in HepG2 cell and HCT-116 cell, the expression trend of selenoproteins and key metabolic enzymes with increased SeMet concentration is similar in L02 cells, which is a parabola with slight differences at the top. The corresponding SeMet concentration for the inflection point of GPx1, SELENOP, PHGDH and SHMT1 is 0.075µmol/L, 0.05µmol/L, 0.05µmol/L and 0.05µmol/L respectively. Also in L02 cell cultured in high-Se media (added with 0.05µmol/L SeMet), there were dose-dependent feedback inhibition effects of exogenous serine or glycine on the expressions of PHGDH. Meanwhile there were promotional effects of exogenous serine or glycine on the expressions of GPx1 and SELENOP.
Conclusions: The L02 cell line is the most suitable for the studies of selenoprotein expression and regulation in vitro among the three kinds of cell lines. Under high-Se stress, the serine de novo synthetic pathway might be utilized to synthesize more endogenous serine and could be feedback inhibited by exogenous serine or glycine. Further research works need to be taken based on the well-tested high-Se induced IR animal models.
Risk of postpartum depressive symptoms is influenced by psychological burden related to the COVID-19 pandemic and dependent from individual stress coping
Authors: Meister Sarah , Dreyer Eva-Maria , Hahn Laura , Thomann Marilena , Keilmann Lucia , Beyer Susanne , Mayer Clarissa , Prins Gwendolin , Hasbargen Uwe , Mahner Sven , jeschke udo , Kolben Thomas , Burges Alexander
Published: September 2022

Purpose
There are different studies worldwide, which have shown a higher risk of mental disorders due to the COVID-19 pandemic. One aim of this study was to characterize factors influencing the psychological burden related to the COVID-19 pandemic and the impact on postpartum depressive symptoms. Further, the role of individual stress and coping strategies was analyzed in this context.
Material and Methods
131 women, who were in obstetric care at the LMU Clinic Munich, between March and October 2020, were questioned by different self-report questionnaires, before birth, one month, two months and 6 months after birth. A designed questionnaire to evaluate the psychological burden related to the COVID-19 pandemic, a modified version of the Stress and coping inventory (SCI) and the Edinburgh Postnatal Depression Scale (EPDS) were utilized
Results
We could show that the psychological burden related to the COVID-19 pandemic influenced the EPDS score one, two and 6 months after birth. In addition, the prenatal stress and individual coping strategies affected the EPDS and the burden related to the COVID-19 pandemic before and after birth significantly.
Conclusion
An association of the psychological burden related to the COVID-19 pandemic with the risk of developing postpartum depressive symptoms could be shown in this study. In this context, the separation of the partner and the family were recognized as important factors. Furthermore, the SCI was identified as effective screening instrument for mothers before delivery to possibly avoid the development of postpartum depression by early diagnosis and intervention.
Succinate promotes pulmonary fibrosis through GPR91 and predicts death in idiopathic pulmonary fibrosis
Authors: He Yijun , Han Yuanyuan , Zou Lijun , Yao Tingting , Zhang Yan , Lv Xin , Jiang Mao , Long Lingzhi , Li Mengyu , Cheng Xiaoyun , Jiang Guoliang , Peng Zhangzhe , Tao Lijian , Jie Meng
Published: September 2022

Background
IPF has been associated with a notable disruption of cellular metabolism. It is still unknown how cellular energy metabolism problems contribute to the onset of pulmonary fibrosis. In an effort to pinpoint the metabolic factors that contribute to pulmonary fibrosis, we investigated the serum metabolomic changes among IPF patients.
Methods
Metabolomics was performed on serum samples from IPF patients using liquid chromatography-mass spectrometry. Further evidence for the crucial metabolic difference came from an IPF cohort. Multivariate analysis and COX regression analysis confirmed its clinical utility in the diagnosis and prognosis of IPF. Our study explored the potential role of this metabolic molecule in pulmonary fibrosis in mice and cells.
Results
Through metabolomics research, it was discovered that the TCA cycle intermediates changed dramatically in IPF, with succinate being of particular concern. There was an accumulation of serum succinate in a prospective cohort of 55 patients with IPF compared to 19 healthy controls. A high serum succinate level was independently associated with higher rates of disease progression (OR = 13.087, 95% CI (2.819–60.761)) and mortality (HR = 3.418, 95% CI (1.308–8.927)). A succinate accumulation was also observed in mice with lung fibrosis induced by bleomycin (BLM). Exogenous succinate administration exacerbated the lung fibrosis caused by BLM, whereas lowering succinate accumulation lessened the fibrosis. Succinate-specific receptors (GPR91) were also elevated in lung tissues from patients with IPF. Furthermore, GPR91-/- mice were protected against lung fibrosis caused by BLM. Through GPR91, succinate promoted TGF-induced activation of normal human lung fibroblasts in vitro.
Conclusions
The findings demonstrate a previously unknown and highly novel association between energy metabolic reprogramming, succinate, fibroblast activation, and clinical prognosis in IPF.
LncRNA CASC7 acts as a tumor suppressor in bladder cancer through modulating miR-103a-5p/TFPI2 axis.
Authors: XIAO JIANHUA , NING JIN-ZHUO , RUAN YUAN , CHENG FAN
Published: September 2022

Background
It was demonstrated that aberrant expression of long noncoding RNAs (LncRNAs) exerts regulatory effects on several malignancies. The lncRNA CASC7 effect on bladder cancer (BC) was not demonstrated. CASC7 role in BC was investigated in the current study.
Material and Methods
CASC7 expression was investigated. Using a luciferase reporter test, the link between CASC7, miR-103a-5p, and TFPI2 was confirmed. CCK-8 and flow cytometry assay were utilized for detecting apoptosis and cell viability. Transwell and wound healing tests were utilized for evaluating the invasion and migration cell capabilities. For analyzing the TFPI2, p-GSK-3β, β-catenin, and GSK-3β protein level, western blot was utilized.
Results
CASC7 expression greatly decreased in BC cell lines and clinical specimens. In function loss and gain assays, CASC7 prevents migration, proliferation, and invasion of the cell and induces cell death in BC cells, in accordance with research. In addition, CASC7 has been reported to enhance TFPI2 expression and negatively regulate the miR-103a-5p expression. Consequently, TFPI2 as a downstream target of miR-103a-5p and miR-103a-5p as a direct target of CASC7 were investigated. Moreover, over-expression of CASC7 markedly reduced the p-GSK-3β, β-catenin, expression. In contrast, the knockdown of CASC7 had the opposite effect.
Conclusion
Our findings showed that BC CASC7 functions as a tumor suppressor via miR-103a-5p and TFPI2. Its regulatory impact is related to the miR-103a-5p/TFPI2/Wnt/ β-catenin signaling pathway. In the BC treatment, CASC7 can serve as a biomarker or therapeutic target.
An efficient, eco-friendly combined technique of matrix solid-phase dispersion (MSPD)-dispersive liquid–liquid micro-extraction (DLLME) coupled with gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS) for determining polybrominated diphenyl ethers (PBDEs) in vegetables
Authors: Xu Sijie , Wang Junxia , Deng Dengxian , Wang Xuedong , Zhang Zhanen
Published: September 2022

A novel method of MSPD combined with DLLME for detecting 7 PBDEs in vegetables was developed by GC-MS/MS quantitation. Firstly, the freeze-dried vegetables were ground and blended with complex sorbents, and packed into a homemade column. PBDEs were eluted with only small amounts of organic solvent, collected and concentrated. Afterward, DLLME procedure was proceeded through addition of 5 mL of ultrapure water to the conical centrifuge tube for purification and pre-concentration. The optimal conditions in the MSPD and DLLME procedures were evaluated using single factor optimization. The results shown that sample weight was 0.2 g, a mixture of C18 and PSA was used as a composite sorbent with a dispersion ratio of 1:4, the elution solvent was a mixture of 8 mL n-hexane-dichloromethane (1:1,v/v) in MSPD process. After that, DLLME procedure was used 500 µl acetonitrile as dispersant and 35 µl carbon tetrachloride as extractant. The developed method showed a good linearity (R2 > 0.9992) between 1 and 1000 ng·g− 1 for all compounds, and matrix effects between − 3.3% and − 18.2%. Limits of detection (LODs) were between 0.08 and 1.00 ng·g− 1. Furthermore, the average recoveries of PBDEs were between 82.9% and 113.8% for all compounds, except for BDE 183 (between 58.5% and 82.5%) with the RSD of lower than 5.2%. This method proved to be saved-solvent, low-cost, eco-friendly, and efficient for the quantitative determination of PBDEs in actual vegetable samples under optimal conditions.
Common Genetic Abnormalities and Phenotypic Scoring in Saudi Patients with Acute B-Lymphoblastic Leukemia
Authors: Alenazi Maha , Alzahrani Faisal , Msmar Amir H. Msmar , Hassan Fathelrahman
Published: September 2022

Introduction: B-acute lymphoblastic leukemia (B-ALL) is a heterogenous disease that has diverse immature cell phenotypes early detection of high-risk groups. The aim of this study was to predictive scoring system using a fully standardized Euroflow eight-color panel.
Methods:The expression of the different CD markers involved in the B-ALL Euroflow panel was investigated by measuring their positivity, percentage, and median fluorescence intensity.
Results: CD9, CD123, and TdT were used to predict TCF3PBX1 with 80% SN and 100% SP. CD20 and CD66 were used to predict hypoploidy with 63% SN and 100% SP. As a result, no useful discriminative scoring system was developed.
Conclusion: Four scoring systems were proposed for the prediction of the most common cytogenetic abnormalities of Saudi B-ALL
Computerized Diagnosis of Polycystic Ovary Syndrome Using Machine Learning and Swarm Intelligence Techniques
Authors: R Subha , R Nayana B , Radhakrishnan Rekha , P Sumalatha
Published: September 2022

Polycystic Ovary Syndrome (PCOS) is one of the profound causes of infertility in women. Early detection, and treatment is essential in improving the prognosis in patients. The current conditions of fertility in India are skeptical, wherein women are at higher risk. PCOS is one of the major causes of infertility and scales upto 20% of women population in India. This requires a timely and accurate diagnosis which can be accomplished by developing automated diagnosing models. Having noted that the data to be dealt with consists of both clinical and non-clinical inputs, the effective information alone needs to be considered for the diagnosis. This necessitates an intelligent selection of features before diagnosing. Thus, swarm intelligence (SI) for feature selection and machine learning for classification is considered to develop a robust and efficient diagnostic model to detect PCOS condition. Initially, optimal features are selected using statistical approaches namely, correlation and Chi Square test and exhaustive search procedure by recursive elimination. Further, the SI algorithms, Particle Swarm Optimization (PSO) and Flashing firefly (FF) are attempted to identify the optimal number and feasible combination of features. Random forest classifier has been used in the ML model for classification. A comparative analysis of the results is discussed and validated based on the parameters accuracy of training and testing, precision, recall, F1-score, and AUC-ROC. The results reveal that ML models with different feature selection algorithms give best performance for different feature dimensions and the model with PSO based feature selection gives the highest performance with minimum feature size. Also PSO based algorithm evades the problem of redundancy in the feature subset.
Incidence and risk factors of postoperative hyperamylasemia and pancreatitis following total knee arthroplasty: a retrospective study
Authors: Liu Song , Li Fangguo , Hu Wei , Yang Qihao , Zhang Chi , Wang Zhao
Published: September 2022

Background
Postoperative hyperamylasemia and pancreatitis are recognized complications after abdominal and spinal surgeries. The aim of this study is to investigate the incidence and identify risk factors for postoperative hyperamylasemia and pancreatitis following total knee arthroplasty.
Methods
170 patients undergoing total knee arthroplasty were retrospectively identified from our database from January 2017 to January 2021. Patients were divided into normal and hyperamylasemia groups based on the presence of serum amylase level within or greater than the normal range. Postoperative pancreatitis was defined as serum amylase level greater than five times above the normal range. Patient demographics, perioperative parameters were investigated with student t test, chi square test and multivariate logistic regression analysis.
Results
43 patients (25.3%) exhibited postoperative hyperamylasemia while eight patients (4.7%) developed postoperative pancreatitis. One patient (0.6%) was designated as having clinical pancreatitis. More patients with Hypertriglyceridemia (HTG) were noted in hyperamylasemia group (P = 0.009) compared with normal group. Hyperamylasemia group showed higher preoperative serum amylase (74.95 vs 55.62 IU/L, P < 0.001), higher IBL (117.67 vs 77.01 mL, P = 0.040) and longer surgical duration (132.98 vs 107.01 min, P = 0.041). Multivariate logistic analysis revealed that HTG (OR = 0.189, P = 0.006), preoperative serum amylase (OR = 1.042, P < 0.001) and IBL (OR = 1.004, P = 0.022) were independent risk factors for postoperative hyperamylasemia.
Conclusions
A significant percentage of patients developed hyperamylasemia after total knee arthroplasty. Patients with HTG, higher preoperative serum amylase and higher IBL had an increased risk of developing postoperative hyperamylasemia and pancreatitis.
The effect of different iron sources on hemoglobin and myoglobin synthesis, and the key genes related to iron metabolism in skeletal muscle in the weanling piglets
Authors: Xu Qilong , Fan Jiajun , Gu Dianchao , Shi Bingzhao , Wang Dan , Zhu Huiling , Liu Jinsong , Liu Yulan , Kang Ping
Published: September 2022

This study was carried out to investigate the effect of different iron sources on hemoglobin and myoglobin (Mb) synthesis, and the mRNA expression of the key genes related to iron metabolism in skeletal muscle in the piglets. Forty eight piglets (Duroc × Large White × Landrace, 9.39 ± 1.55 kg, 40 ± 2 d) were assigned into four treatments including control group, a basal diet without iron supplemented in mineral premix; ferrous sulfate (FeSO4) group, the basal diet supplemented with FeSO4 to provide 100 mg supplemental Fe/kg dry matter (DM); ferrous glycinate (Fe-Gly) group, the basal diet supplemented with Fe-Gly to provide 80 mg supplemental Fe/kg DM; amino acid-Fe(II)-chelator complexes group, the basal diet supplemented with amino acid-Fe(II)-chelator complexes to provide 30 mg supplemental Fe/kg DM. There were six pens for each treatment, and each pen had two piglets. The experiment was lasted for 28 days. The results showed that different iron source supplementation increased the mean corpuscular hemoglobin in blood and Mb concentration in muscles (P < 0.05). FeSO4 and Fe-Gly supplementation increased the mean corpuscular volume and mean cell hemoglobin concentration in blood (P < 0.05). In addition, different iron source increased hephaestin expression in longissimus dorsi muscle (P < 0.1), decreased transferrin receptor 1 expression in longissimus dorsi muscle (P < 0.05), and increased lipocalin 2 expression in gastrocnemius muscle, respectively (P < 0.05). In summary, iron supplementation has positive effectiveness in myoglobin biosynthesis and can mobilize iron in muscles into the circulation regardless of iron sources.
Dynesys versus Dynamic Hybrid System in the treatment of Multi-Segmental Lumbar Degenerative Disease: a retrospective study
Authors: Zhang Xin , Xiao Xiao , Wang Hongyu , Wang Song , Yang Dazhi , Peng Songlin
Published: September 2022

Background

Of all the spinal diseases, lumbar degenerative disease (LDD) is a common and frequently-occurring disease and often develops into multi-segmental LDD over time. When multi-segment LDD occurs in middle-aged people who love sports, dynamic internal fixation, which can preserve the range of motion (ROM) and prevent adjacent segment degeneration (ASD), is more suitable for such people than interbody fusion. Nowadays, researchers have mainly focused on comparing the effectiveness of hybrid fixation versus rigid fusion, or Dynesys fixation versus rigid fusion, whereas Dynesys fixation and hybrid fixation have not been adequately compared in multi-segmental LDD in terms of clinical and radiographic evaluations.

Methods

We included 35 patients with multi-segmental LDD from January 2015 to August 2019, divided into Dynesys (only used Dynesys system, n = 22) and Hybrid (used Dynesys system and an intervertebral cage, n = 13) groups. Clinical outcomes were evaluated using perioperative data, Oswestry Disability Index (ODI), and Visual Analogue Scale (VAS). Radiologic evaluations included lumbar spine X-ray, MRI, and CT. Furthermore, different complications were analyzed.

Results

At the last follow-up, ODI and VAS of each group were significantly improved (p < 0.05), and the ROM of operating segments decreased. However, Dynesys group preserved a larger extent of ROM than that of Hybrid group at the final follow-up (p < 0.05). ROM of the upper adjacent segment was increased in both groups (p < 0.05), while the disc heights were significantly decreased at the final follow-up than those one-week post-surgery (p < 0.05). Besides, Dynesys group had a more obvious decrease in the disc height of dynamic segments than Hybrid group (p < 0.05). No significant difference existed in complications between both groups (p > 0. 05).

Conclusion

Dynesys and hybrid fixations can preserve ROM of stabilized segments. Dynesys fixation can preserve a larger extent of ROM than that of Hybrid fixation. However, hybrid fixation can better preserve the disc height of the non-fusion level. Overall, both surgical procedures can be employed as effective treatments for middle-aged and physically active patients with multi-segmental LDD.
Effect of Cryothermic and Radiofrequency Cox-Maze IV Ablation on Atrial Size and Function Assessed by 3D Echocardiography, A Randomized Trial. To Freeze or to Burn
Authors: Boano Gabriella , Vanky Farkas , Aneq Meriam Åström
Published: September 2022

BACKGROUND
Cox-maze procedures are safe methods of restoring sinus rhythm (SR) in patients with atrial fibrillation (AF), and are often performed concomitant with mitral valve (MV) surgery. Atrial linear scars are achieved using Cryothermy (Cryo) or radiofrequency (RF) techniques. It is unclear how these energy sources differ in terms of effects on late left atrial (LA) reverse remodeling. We used 3-dimensional echocardiography (3DE) to compare the impact of Cryo and RFprocedures on LA size and function one year after Cox-maze IV ablation concomitant with MV surgery.
METHODS
Seventy-two patients with MV disease and AF were randomized to Cryo (n = 35) or RF (n = 37) ablation. Another 33 patients were enrolled according to the protocol without ablation (NoMaze). All patients underwent echocardiogram before and one year after surgery. Between-group differences were evaluated using the Student’s paired t-test. Fisher’s analysis was used for categorical data.
RESULTS
Forty-two ablated patients recovered sinus rhythm (SR) one year post-surgery, and had comparable left and right systolic ventricular function and a mean LA size reduction of 23% (LA volume index decreased from 66 ± 24 to 54 ± 13 ml/m2; p = 0.004). The 3DE extracted reservoir and booster function were higher after RF (37 ± 10% vs. 26 ± 6%; p < 0.001) than Cryo ablation (18 ± 9 vs. 7 ± 4%; p < 0.001), while passive conduit function was comparable between groups (24 ± 11 vs. 20 ± 8%; p = 0.17). Compared to maze groups, patients with restored SR without ablation (7 patients) had similar LA size reduction but more preserved systolic atrial function. The level of LAVI reduction depended on AF duration. Up to 30% reduction in 60% of patients with documented AF less than 6 months compared to 11% in those with AF for more than 5 years.
CONCLUSIONS
SR restoration after mitral surgery and maze resulted in LA size reduction, irrespective of the applied energy source. Using 3DE, we demonstrated that compared to RF, Cryo produced an extended ablation area that implies LA structural remodeling affecting LA systolic function. Reduced reverse size remodeling was associated with longer AF history.
TRIAL REGISTRATION
DOI 10.1186/ISRCTN14454361.
FY-3E GNOS-R: a new illustration of global soil moisture monitoring
Authors: Yang Guanglin , Du Xiaoyong , Huang Lingyong , Bai Weihua , Sun Yueqiang , Xia Junming , Yin Cong , Huang Feixiong , Wu Xuerui , Liu Yan , Zhang Peng , Hu Xinqing , Sun Ling , Qi Chengli , Zhang Xiaoxin , Wang Jinsong
Published: September 2022

The Fengyun-3E (FY-3E), which is the first early-morning-orbit meteorological satellite, was launched by China on July 10, 2021. One of its 11 payloads is the GNSS Occultation Sounder II (GNOS-II), which is a combination of Global Navigation Satellite System (GNSS) Radio Occultation and GNSS reflectometry (GNSS-R) sensor. An important scientific goal of GNOS reflectometry (GNOS-R) is soil moisture detection. Different from previous GNSS-R missions, such as CYGNSS (Cyclone Global Navigation Satellite System), the coverage of FY-3E GNOS-R is pan-global (±85◦ latitude), providing an opportunity for global soil moisture estimation. Owing to data volume limitations, soil moisture retrieval using artificial intelligence is not employed; instead, soil moisture estimation is based on physical scattering models. The LAnd surface GNSS Reflection Simulator (LAGRS) is a spaceborne GNSS-R simulator designed specifically for FY-3E GNOS-R, which provides corresponding theoretical values of surface reflectivity. To ensure the accuracy of soil moisture estimation, the retrieval process includes not only calibration but also removal of surface roughness and vegetation effects. Using the LAGRS model, we obtained the calibration factor and roughness–vegetation factor, and with the mask of Fresnel reflectivity, we achieved soil moisture retrieval. During the entire retrieval process, The Soil Moisture Active Passive (SMAP) ancillary global soil moisture data were treated as reference values. A reasonable degree of consistency was found between the FY-3E GNOS-R soil moisture retrievals and the SMAP data (correlation coefficient: 0.9599, root mean square error: 0.0483 cm3/cm3). This study represents the first illustration of global soil moisture retrieval based on physical models using FY-3E GNOS-R.
A Dodgson-Hare Synthesis
Authors: Green-Armytage James
Published: September 2022

In 1876, Charles Dodgson (better known as Lewis Carroll) proposed a committee election procedure that chooses the Condorcet winner when one exists and otherwise eliminates candidates outside the Smith set, then allows for re-votes until a Condorcet winner emerges. The present paper discusses Dodgson’s work in the context of strategic election behavior and suggests a “Dodgson-Hare” method, a variation on Dodgson’s procedure, for use in public elections. This method allows for candidate withdrawal and employs Hare’s plurality-loser-elimination method to resolve persistent cycles. Given plausible assumptions about how candidates decide whether to withdraw when there is a cycle, Dodgson-Hare outperforms Hare, Condorcet-Hare, and 12 other voting rules in a series of spatial-model simulations that count how often each rule is vulnerable to coalitional manipulation. In the case of a one-dimensional spatial model, all coalitional voting strategies that are possible under Condorcet-Hare can be undone in Dodgson-Hare, by the withdrawal of candidates who have incentive to withdraw.
JEL Classifications: D71, D72
Body dissatisfaction and social anxiety among adolescents: A moderated mediation model of feeling of inferiority, family cohesion and friendship quality
Authors: Dou Qianqian , Chang Ruosong , Xu Hui
Published: September 2022

Previous research has documented that body dissatisfaction is positively related to social anxiety. However, little is known about the mediating and moderating mechanisms underlying this relation. Based on ecological systems theory, this study aimed to examine the chain mediation effect of friendship quality and family cohesion in the relationship between body dissatisfaction and social anxiety among adolescents and whether this mediating process was moderated by feeling of inferiority. A total of 972 students (mean age = 15.77 years, SD = 0.856) were enrolled in this study. All participants completed questionnaires regarding adolescent body dissatisfaction, feeling of inferiority, family cohesion, friendship quality and social anxiety. Our study found that body dissatisfaction was positively associated with social anxiety. Feeling of inferiority and family cohesion were found to play a chain-mediated role in the relation between body dissatisfaction and social anxiety. In addition, friendship quality plays a moderating role in the relationship between body dissatisfaction and feeling of inferiority. According to ecological systems theory, it suggested that family cohesion and friendship quality, as important elements in the microsystem of adolescents, interacted with each other, and interacted with the core parts of the ecosystem, such as body dissatisfaction and feeling of inferiority. These effects in turn have an impact on the individual's psychological characteristics-social anxiety. These findings show lights on how body dissatisfaction is associated with social anxiety in adolescents.
Moral distress in psychiatric nurses in Covid-19 crisis
Authors: tavakol nahid , molazem zahra , rakhshan mahnaz , asemani omid , bagheri shahpar
Published: September 2022

Background: The COVID-19 epidemic has put heavy pressure on nurses. Psychiatric nurses are also exposed to moral distress due to the special conditions of psychiatric patients and patient’s lack of cooperation in observing health protocols.
This study has been conducted to explore and describe factors that caused moral distress in psychiatric nurses during the COVID-19 epidemic.
Method: This qualitative study with a conventional content analysis approach involved 12 nurses at Shiraz University of Medical Sciences in Iran. This study was conducted in the winter of 2021. Data collection was performed by unstructured interviews, data analysis was performed based on the five steps of Graneheim and Lundman.
Results: From the data analysis, 252 initial codes were extracted, which were obtained by continuous comparison and integration of subcategories of 4 main categories including emotional responses, relational factors, Institutional factors, and management factors caused by Moral Distress in psychiatric nurses.
Conclusion: new dimensions of the causes of moral distress associated with the COVID-19 pandemic are discovered. Managers and planners should equip psychiatric hospitals with isolation facilities and Personal Protection Equipment for patients and nurses. Strengthening the ethical climate by improving communication skills and individual nursing empowerment to prevent moral distress is recommended.
Development and Validation of the COPD Scale among the System of Quality of Life Instruments for Chronic Diseases QLICD-COPD (V2.0)
Authors: Pan Haiyan , Yang Zheng , Zhao Zhihuan , Lin Yue , Wu Bin , Pan Congcong , Wan Chonghua
Published: September 2022

Background Chronic obstructive pulmonary
disease (COPD) is a chronic disease with high mortality and severe
morbidity that keep increasing the burden of the health of the
population. Chronic Obstructive Pulmonary Disease (QLICD-COPD)
(V2.0) was used to measure the quality of life for patients with
COPD. The study aimed to develop the instruments to evaluate a
quality-of-life questionnaire for COPD.

Methods The questionnaires were developed
after reviewing the literature and three hundred and twenty-one
patients with COPD were evaluated by a series of statistical
methods consisting of the paired t-test, correlation analysis, and
factor analysis.

Results The overall Cronbach’s alpha
coefficient was 0.889 (greater than the accepted reference value of
0.7), and the coefficients of most domains and facets were in the
range 0.6 to 0.88, except some facets of psychological domain and
social domain. The split-half reliability coefficients of the
domains and the total scale is greater than 0.6 apart from the
physiological domain. Intra-domain internal consistency
coefficients were higher than inter-domain correlation coefficients
and each item was more strongly correlated with its domain than
with other domains. SF-36 domains with high reliability and
validity closed linked with the scores of QLICD-COPD (2.0) except
the general health and social domains. The paired t-test
showed significant changes in the scores of most domains after
treatment with P<0.001, and the domains and facets with
significant score changes after treatment obtained relatively large
standardized response mean(SRM).

Conclusion The QLICD-COPD (V2.0) questionnaire
shows good reliability, validity, and responsiveness, and can serve
as an instrument for evaluating the quality of life of patients
with COPD in China.
High Expression of GMNN Predicts Malignant Progression and Poor Prognostic in ACC
Authors: Zhao Xinzhao , Zhang Xuezhou , Shao Shixiu , Yang Qingbo , Shen Chengquan , Yang Xuecheng , Jiao Wei , Liu Jing , Wang Yonghua
Published: September 2022

Background
Adrenocortical carcinoma (ACC) is a rare endocrine neoplasm, which is characterized by poor prognosis and high recurrence rate. Novel and reliable prognostic and metastatic biomarkers are lacking for ACC patients. This study aims at screening potential prognostic biomarkers and therapeutic targets of ACC through bioinformatic methods and immunohistochemical (IHC) analysis.
Methods
In the present study, by using the Gene Expression Omnibus (GEO) database we identified differentially expressed genes (DEGs) in ACC and validated these DEGs in The Cancer Genome Atlas (TCGA) ACC cohort. A DEGs-based signature was additionally constructed and we assessed its prognostic and prescient worth for ACC by survival analysis and nomogram. Immunohistochemistry (IHC) was used to verify the relationship between hub gene-GMNN expressions and clinicopathologic outcomes in ACC patients.
Results
A total of 24 DEGs correlated with the prognosis of ACC were screened from the TCGA and GEO databases. Five DEGs were subsequently selected in a signature which was closely related to the survival rates of ACC patients. Among these genes, GMNN was identified as a hub gene and was independently associated with the survival of ACC. Meanwhile, in our cohort we also found that GMNN was significantly overexpressed in tumor tissues and was closely related to the pathological features and prognostic of ACC.
Conclusions
GMNN is a novel tumor marker for predicting the malignant progression, metastasis and prognosis of ACC, and may be a potential therapeutic target for ACC.