bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptive robotic system for the inspection of aerospace slat actuator mount
Authors: Morsi Nour M. , Mata Mario , Harrison Colin S. , Semple David
Published: June 2024

Introduction: Robotics uptake in the aerospace industry is low, mainly due to the low-volume/high-accuracy production that aerospace manufacturers require. Furthermore, aerospace manufacturing and assembly sites are often unstructured environments not specifically suitable for robots to operate in.

Methods: This paper introduces a robotic visual inspection system using off-the-shelf components able to inspect the mounting holes for wing slat actuators without the need for fixed-coordinate programming; the part just needs to be left within reach of the robot. Our system sets one of the opposed pairs of mounting holes as a reference (the “datum”) and then compares the tilt of all other pairs of mounting holes with respect to it. Under the assumption that any deviation in the mounting hole tilt is not systematic but due to normal manufacturing tolerances, our system will either guarantee the correct alignment of all mounting holes or highlight the existence of misaligned holes.

Results and Discussion: Computer-vision tilt measurements are performed with an error of below 0.03° using custom optimization for the sub-pixel determination of the center and radius of the mounting holes. The error introduced by the robot’s motion from the datum to each of the remaining hole pairs is compensated by moving back to the datum and fixing the orientation again before moving to inspect the next hole pair. This error is estimated to be approximately 0.05°, taking the total tilt error estimation for any mounting hole pair to be 0.08° with respect to the datum. This is confirmed by manually measuring the tilt of the hole pairs using a clock gauge on a calibrated table (not used during normal operation).
Temporal Trends in Tobacco Smoking Prevalence During the Period 2010–2020 in Vietnam: A Repeated Cross-Sectional Study
Authors: Vu Lan Thi Hoang , Bui Quyen Thi Tu , Shelley Donna , Niaura Raymond , Tran Bao Quoc , Pham Nga Quynh , Nguyen Lam Tuan , Chu Annie , Pratt Angela , Thi Lan Pham Chi , Hoang Minh Van
Published: June 2024

Objectives: This study used repeated cross-sectional data from three national surveys in Vietnam to determine tobacco smoking prevalence from 2010 to 2020 and disparities among demographic and socioeconomic groups.

Methods: Tobacco smoking temporal trends were estimated for individuals aged 15 and over and stratified by demographic and socioeconomic status (SES). Prevalence estimates used survey weights and 95% confidence intervals. Logistic regression models adjusted for survey sample characteristics across time were used to examine trends.

Results: Tobacco smoking prevalence dropped from 23.8% in 2010 to 22.5% in 2015 and 20.8% in 2020. The adjusted OR for 2015 compared to 2010 was 0.87, and for 2020 compared to 2010 was 0.69. Smoking decreased less for employed individuals than unemployed individuals in 2020 compared to 2010. Smoking was higher in the lower SES group in all 3 years. Higher-SES households have seen a decade-long drop in tobacco use.

Conclusion: This prevalence remained constant in lower SES households. This highlights the need for targeted interventions to address the specific challenges faced by lower-SES smokers and emphasizes the importance of further research to inform effective policies.
The Role of Crop Wild Relatives and Landraces of Forage Legumes in Pre-Breeding as a Response to Climate Change
Authors: Perić Katarina , Čupić Tihomir , Krizmanić Goran , Tokić Branimir , Andrić Luka , Ravlić Marija , Meglič Vladimir , Tucak Marijana
Published: June 2024

Climate change and the variable extreme weather conditions that today’s agricultural producers are dealing with represent some of the most important issues in food production. Nowadays, weather patterns are increasingly unpredictable, characterized by frequent fluctuations in temperature, precipitation, and extreme weather events. As a result, there is an increasing demand for scientists to develop more resilient and tolerant crop cultivars. Plant breeders must become creative and utilize all available resources to create modern high-yielding and widely adapted cultivars to help agriculture grow and thrive amidst the emerging changes. Forage legumes, due to their beneficial characteristics, are among the crops that can contribute to mitigating the consequences of climate change. Furthermore, what certainly does not contribute to weather conditions is the erosion of plant genetic material, which has been caused by the modernization of agriculture and the selection of the best cultivars with desirable traits over many years. Crop wild relatives (CWRs) and landraces represent plant genetic materials rich in novel gene variants that contain traits for resistance and tolerance to different climatic conditions. To expand the genetic base of cultivars and mitigate the consequences of climate change, breeders are increasingly utilizing pre-breeding methods. These methods include all the activities connected to the identification of desirable genes and traits from un-adapted materials, such as CWRs, and the transformation of these traits to an intermediate set of materials that can be used for creating new cultivars. This review paper will cover the pre-breeding process, including its components, and the resistance and/or tolerance of the CWRs and landraces of forage legumes to different extreme environmental conditions.
Ruminant Grazing Lands in the Tropics: Silvopastoral Systems and Tithonia diversifolia as Tools with Potential to Promote Sustainability
Authors: Krüger Ana Maria , Lima Paulo de Mello Tavares , Ovani Vagner , Pérez-Marquéz Simón , Louvandini Helder , Abdalla Adibe Luiz
Published: June 2024

Food security, sustainability of food production, and greenhouse gas (GHG) production of ruminant livestock are topics that generate scrutiny and debates worldwide. In a scenario of increasing human population and concerns with climate change, it is necessary to increase animal-derived food in sustainable operations. Grazing systems are crucial for ruminant production worldwide, and in the tropics, well-managed grasslands can provide sustainable intensification of this activity. In these regions, production often relies on grass monoculture managed extensively, a practice that commonly has led to the occurrence of degraded soils, limited animal productivity, and increased intensity of GHG emissions. Silvopastoralism is a practice that promotes several ecosystem services, showing potential to maintain soil quality while reducing the environmental impacts of ruminant production. These systems also have the potential to improve animal productive performance and reduce GHG emissions. The review was guided by a search in the Web of Science database using population terms and refined by document type (Article) and language (English OR Portuguese) following PRISMA protocol. Infographics were created using the Bibliometrix package in R software (version 4.3.2), and a specific topic on Tithonia diversifolia (Hemsl.) A. Gray was explored to demonstrate the importance of this forage resource for tropical silvopastoral systems and its potential contribution to food security. The T. diversifolia shrub is widely distributed in Latin America and tropical regions and presents several characteristics that make it a good option for silvopastoral systems. Focusing on the tropics, our objectives were to present one literature review addressing the role of grazing ruminant production towards the current climate change and food security challenges. Additionally, we aimed to explore the state of knowledge on silvopastoral systems and the use of T. diversifolia, presenting their potential to cope with this scenario of increased concerns with the sustainability of human activities.
Comparison of Antibiotic Use and the Frequency of Diseases Depending on the Size of Herd and the Type of Cattle Breeding
Authors: Kupczyński Robert , Bednarski Michał , Sokołowski Marcin , Kowalkowski Wojciech , Pacyga Katarzyna
Published: June 2024

Diseases are responsible for losses in livestock production by increasing animal mortality and reducing productivity. The administration of antibiotics can help mitigate these negative effects. However, inappropriate use can lead to severe complications, such as raising antibiotic resistance. The purpose of this study was to perform a comparative analysis of antibiotic use and disease frequency over four years, based on the size of dairy farms and the type of farm. The study covered a 4-year period and included medium dairy farms (20–50 cows, n = 13), large dairy farms (>250 cows, n = 8), and large beef farms (n = 8). The collected data involved antimicrobial use but also included farm demographics, animal health, disease frequency, and herd management practices. The criteria used to categorise antibiotics into groups A–D were based on the EMA guidelines. The carried-out study showed that the large dairy cattle farms had the highest antibiotic consumption (18.29 mg·PCU−1), due to the high frequency of diseases, and consequently, the treatment of calf (diarrhoea, lung inflammations) and cow diseases (general treatment and mastitis). Cattle on large beef farms suffer mainly from general diseases caused by maintenance and herd management conditions. The use of restrict antibiotics was, in some cases, unjustified (antibiotics for dry cow therapy). Future studies should consider a larger number of farms, taking into account the given direction of cattle production.
The Simulated Physiological Oocyte Maturation (SPOM) System Enhances Cytoplasmic Maturation and Oocyte Competence in Cattle
Authors: Navarro Micaela , Fanti Tomás , Ortega Nicolas Matias , Waremkraut Magalí , Guaimas Francisco , Mutto Adrian Ángel , Blüguermann Carolina
Published: June 2024

In vitro embryo production is a widely applied technique that allows the expansion of genetics and accelerated breeding programs. However, in cattle, this technique still needs improvement in order to reach quality and pregnancy rates comparable to in vivo-derived embryos. One of the limitations of this technique is related to in vitro maturation, where a heterogeneous population of oocytes is harvested from follicles and cultured in vitro in the presence of gonadotropic hormones to induce maturation. As a result, oocytes with different degrees of competence are obtained, resulting in a decrease in the quality and quantity of embryos obtained. A novel system based on the use of cyclic adenosine monophosphate (cAMP) modulators was developed to enhance bovine oocyte competence, although controversial results were obtained depending on the in vitro embryo production (IVP) system used in each laboratory. Thus, in the present work, we employed a reported cAMP protocol named Simulated Physiological Oocyte Maturation (SPOM) under our IVP system and analysed its effect on cytoplasmic maturation by measuring levels of stress-related genes and evaluating the activity and distribution of mitochondria as a marker for cytoplasmic maturation Moreover, we studied the effect of the cAMP treatment on nuclear maturation, cleavage, and blastocyst formation. Finally, we assessed the embryo quality by determining the hatching rates, total cell number per blastocyst, cryopreservation tolerance, and embryo implantation. We found that maturing oocytes in the presence of cAMP modulators did not affect nuclear maturation, although they changed the dynamic pattern of mitochondrial activity along maturation. Additionally, we found that oocytes subjected to cAMP modulators significantly improved blastocyst formation (15.5% vs. 22.2%, p < 0.05). Blastocysts derived from cAMP-treated oocytes did not improve cryopreservation tolerance but showed an increased hatching rate, a higher total cell number per blastocyst and, when transferred to hormonally synchronised recipients, produced pregnancies. These results reflect that the use of cAMP modulators during IVM results in competent oocytes that, after fertilisation, can develop in more blastocysts with a better quality than standard IVM conditions.
Bacterial Contamination of Environmental Surfaces of Veterinary Rehabilitation Clinics
Authors: Spratt Henry G. , Millis Nicholas , Levine David , Brackett Jenna , Millis Darryl
Published: June 2024

The presence of potentially pathogenic bacteria on veterinary clinic surfaces may be problematic. In this study, we collected swab samples (Fisherbrand, double transport swabs with Stuart’s liquid medium) and water samples from five veterinary rehabilitation clinics. Swabs and water samples were transported to a microbiology lab for processing. At the lab, swabs were used to inoculate Hardy’s Cdiff Banana Broth (for Clostridium difficile [Cdiff]) and five different types of bacterial growth media, including Hardy CHROM MRSA agar (methicillin-resistant Staphylococcus aureus [MRSA] and S. pseudintermedius [SIM]), mannitol salt agar (S. aureus [SA]), eosin methylene blue agar (enterics [ENT]), Pseudomonas isolation agar (Pseudomonas spp. [PS]), and tryptic soy agar [TSA] (non-specific). The most prominent presumptive species cultured was Cdiff (on nearly 55% of swabs). Bacillus spp. and enteric bacteria were encountered on nearly 35% of swabs, with MRSA and SIM on just over 10% of swabs. The most contaminated sample site was harnesses/life jackets used with the underwater treadmill (33% of swabs). The underwater treadmill water had total bacterial counts from 1,600 to 2,800 cfu/mL. Of all presumptive bacterial species detected, SIM tends to be more pathogenic for dogs. Targeted cleaning/disinfecting in these clinics could help reduce risks for both animals and caregivers utilizing these clinics.
Long Non-Coding RNAs Differentially Expressed in Swine Fetuses
Authors: Campos Francelly G. , Ibelli Adriana M. G. , Cantão Maurício E. , Oliveira Haniel C. , Peixoto Jane O. , Ledur Mônica C. , Guimarães Simone E. F.
Published: June 2024

Long non-coding RNAs (lncRNAs) are non-coding transcripts involved in various biological processes. The Y chromosome is known for determining the male sex in mammals. LncRNAs on the Y chromosome may play important regulatory roles. However, knowledge about their action mechanisms is still limited, especially during early fetal development. Therefore, we conducted this exploratory study aiming to identify, characterize, and investigate the differential expression of lncRNAs between male and female swine fetuses at 35 days of gestation. RNA-Seq libraries from 10 fetuses were prepared and sequenced using the Illumina platform. After sequencing, a data quality control was performed using Trimmomatic, alignment with HISAT2, and transcript assembly with StringTie. The differentially expressed lncRNAs were identified using the limma package of the R software (4.3.1). A total of 871 potentially novel lncRNAs were identified and characterized. Considering differential expression, eight lncRNAs were upregulated in male fetuses. One was mapped onto SSC12 and seven were located on the Y chromosome; among them, one lncRNA is potentially novel. These lncRNAs are involved in diverse functions, including the regulation of gene expression and the modulation of chromosomal structure. These discoveries enable future studies on lncRNAs in the fetal stage in pigs.
Effects of Freshwater Acidification on the Gut Microbial Community of Trachemys scripta elegans
Authors: Niu Xin , Dang Zhaohui , Hong Meiling , Shi Haitao , Ding Li
Published: June 2024

Freshwater acidification (FA) has become a global environmental problem, posing a potential threat to freshwater ecosystems. The gut microbiota plays a crucial role in the host’s response and adaptation to new environments. In this study, we investigated the changes in microbial communities in Red-eared slider (Trachemys scripta elegans) under acidic conditions to reveal the ecological impacts of acidification on freshwater turtles. The results showed that there were significant differences in β-diversity (p = 0.03), while there were no significant differences in the α-diversity of gut microbiota in T. s. elegans between the different levels of acidification (pH of 5.5, 6.5, 7.5). Both the Gut Microbiome Health Index (GMHI) and the Microbial Dysbiosis Index (MDI) exhibited significant differences when comparing environments with a pH of 5.5 to those with a pH of 6.5 (p < 0.01). A comparative analysis between pH levels of 5.5 and 6.5 also revealed substantial differences (p < 0.01). Likewise, a comparative analysis between pH levels of 6.5 and 7.5 also revealed substantial differences (p < 0.01). At the phylum level, Firmicutes, Fusobacteria, and Bacteroidota formed a major part of the gut microbial community, Fusobacteria showed significant differences in different acidity environments (p = 0.03). At the genus level, Cetobacterium, Turicibacter, unclassified Eubacteriaceae, and Anaerorhabdus_furcosa_group showed significant differences in different acidity environments. The pH reduced interactivity in the gut microbiota of T. s. elegans. In addition, LEfSe analysis and functional prediction revealed that the potentially_pathogenic and stress_tolerant functional characteristics also showed significant differences in different acidity environments. The findings underscore the pivotal role of the gut microbiota in T. s. elegans in response to freshwater acidification and provide a foundation for further exploration into the impacts of acidification on freshwater ecosystems.
Cryptosporidium spp. Infection and Genotype Identification in Pre-Weaned and Post-Weaned Calves in Yunnan Province, China
Authors: Deng Meng-Ling , Heng Zhao-Jun , Li Liu-Jia , Yang Jian-Fa , He Jun-Jun , Zou Feng-Cai , Shu Fan-Fan
Published: June 2024

Background: Cryptosporidium is a globally distributed zoonotic protozoan parasite in humans and animals. Infection is widespread in dairy cattle, especially in calves, resulting in neonatal enteritis, production losses and high mortality. However, the occurrence of Cryptosporidium spp. in pre- and post-weaned calves in Yunnan Province remains unclear. Methods: We collected 498 fecal samples from Holstein calves on 10 different farms in four regions of Yunnan Province. Nested PCR and DNA sequencing were used to determine the infection, species and genotypes of Cryptosporidium spp. in these animals. Results: The overall occurrence of Cryptosporidium spp. in Holstein calves was 32.9% (164/498), and the prevalence in pre- and post-weaned calves was 33.5% (106/316) and 31.9% (58/182), respectively. Four Cryptosporidium species were identified in these animals, namely C. bovis (n = 119), C. parvum (n = 23), C. ryanae (n = 20) and C. andersoni (n = 2). Based on sequencing analysis of the 60 kDa glycoprotein gene of C. bovis, C. parvum and C. ryanae, six subtypes of C. bovis (XXVIe, XXVIb, XXVIf, XXVIa XXVIc and XXVId), two subtypes of C. parvum (IIdA19G1 and IIdA18G1) and four subtypes of C. ryanae (XXIf, XXId, XXIe and XXIg) were identified. Conclusions: These results provide essential information to understand the infection rate, species diversity and genetic structure of Cryptosporidium spp. populations in Holstein pre-weaned and post-weaned calves in Yunnan Province. Further, the presence of IIdA18G1 and IIdA19G1 in C. parvum implies significant animal and public health concerns, which requires greater attention and more preventive measures.
Development and ELISA Characterization of Antibodies against the Colistin, Vancomycin, Daptomycin, and Meropenem: A Therapeutic Drug Monitoring Approach
Authors: Garzon Vivian , Salvador J.-Pablo , Marco M.-Pilar , G.-Pinacho Daniel , Bustos Rosa-Helena
Published: June 2024

More than 70% of bacteria are resistant to all or nearly all known antimicrobials, creating the need for the development of new types of antimicrobials or the use of “last-line” antimicrobial therapies for the treatment of multi-resistant bacteria. These antibiotics include Glycopeptide (Vancomycin), Polymyxin (Colistin), Lipopeptide (Daptomycin), and Carbapenem (Meropenem). However, due to the toxicity of these types of molecules, it is necessary to develop new rapid methodologies to be used in Therapeutic Drug Monitoring (TDM). TDM could improve patient outcomes and reduce healthcare costs by enabling a favorable clinical outcome. In this way, personalized antibiotic therapy emerges as a viable option, offering optimal dosing for each patient according to pharmacokinetic (PK) and pharmacodynamic (PD) parameters. Various techniques are used for this monitoring, including high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and immunoassays. The objective of this study is the development and characterization by ELISA of specific polyclonal antibodies for the recognition of the antibiotics Vancomycin (glycopeptide), Colistin (polymyxin), Daptomycin (lipopeptide), and Meropenem (carbapenem) for future applications in the monitoring of these antibiotics in different fluids, such as human plasma. The developed antibodies are capable of recognizing the antibiotic molecules with good detectability, showing an IC50 of 0.05 nM for Vancomycin, 7.56 nM for Colistin, 183.6 nM for Meropenem, and 13.82 nM for Daptomycin. These antibodies offer a promising tool for the precise and effective therapeutic monitoring of these critical antibiotics, potentially enhancing treatment efficacy and patient safety.
Dramatic Suppression of Lipogenesis and No Increase in Beta-Oxidation Gene Expression Are among the Key Effects of Bergamot Flavonoids in Fatty Liver Disease
Authors: Parafati Maddalena , La Russa Daniele , Lascala Antonella , Crupi Francesco , Riillo Concetta , Fotschki Bartosz , Mollace Vincenzo , Janda Elzbieta
Published: June 2024

Bergamot flavonoids have been shown to prevent metabolic syndrome, non-alcoholic fatty liver disease (NAFLD) and stimulate autophagy in animal models and patients. To investigate further the mechanism of polyphenol-dependent effects, we performed a RT2-PCR array analysis on 168 metabolism, transport and autophagy-related genes expressed in rat livers exposed for 14 weeks to different diets: standard, cafeteria (CAF) and CAF diet supplemented with 50 mg/kg of bergamot polyphenol fraction (BPF). CAF diet caused a strong upregulation of gluconeogenesis pathway (Gck, Pck2) and a moderate (>1.7 fold) induction of genes regulating lipogenesis (Srebf1, Pparg, Xbp1), lipid and cholesterol transport or lipolysis (Fabp3, Apoa1, Lpl) and inflammation (Il6, Il10, Tnf). However, only one β-oxidation gene (Cpt1a) and a few autophagy genes were differentially expressed in CAF rats compared to controls. While most of these transcripts were significantly modulated by BPF, we observed a particularly potent effect on lipogenesis genes, like Acly, Acaca and Fasn, which were suppressed far below the mRNA levels of control livers as confirmed by alternative primers-based RT2-PCR analysis and western blotting. These effects were accompanied by downregulation of pro-inflammatory cytokines (Il6, Tnfa, and Il10) and diabetes-related genes. Few autophagy (Map1Lc3a, Dapk) and no β-oxidation gene expression changes were observed compared to CAF group. In conclusion, chronic BPF supplementation efficiently prevents NAFLD by modulating hepatic energy metabolism and inflammation gene expression programs, with no effect on β-oxidation, but profound suppression of de novo lipogenesis.
Diurnal-Rhythmic Relationships between Physiological Parameters and Photosynthesis- and Antioxidant-Enzyme Genes Expression in the Raphidophyte Chattonella marina Complex
Authors: Mukai Koki , Qiu Xuchun , Takai Yuki , Yasuo Shinobu , Oshima Yuji , Shimasaki Yohei
Published: June 2024

Diurnal rhythms in physiological functions contribute to homeostasis in many organisms. Although relationships between molecular biology and diurnal rhythms have been well studied in model organisms like higher plants, those in harmful algal bloom species are poorly understood. Here we measured several physiological parameters and the expression patterns of photosynthesis-related and antioxidant-enzyme genes in the Chattonella marina complex to understand the biological meaning of diurnal rhythm. Under a light–dark cycle, Fv/Fm and expression of psbA, psbD, and 2-Cys prx showed significant increases in the light and decreases during the dark. These rhythms remained even under continuous dark conditions. DCMU suppressed the induction of psbA, psbD, and 2-Cys prx expression under both light regimes. Oxidative stress levels and H2O2 scavenging activities were relatively stable, and there was no significant correlation between H2O2 scavenging activities and antioxidant-enzyme gene expression. These results indicate that the Chattonella marina complex has developed mechanisms for efficient photosynthetic energy production in the light. Our results showed that this species has a diurnal rhythm and a biological clock. These phenomena are thought to contribute to the efficiency of physiological activities centered on photosynthesis and cell growth related to the diurnal vertical movement of this species.
Optimizing Cellulase—Limosilactobacillus fermentum ZC529 Synergy Fermentation for Preserving Macadamia integrifolia Pericarp’s Potential Use as Antioxidants
Authors: Zhang Chen , Huang Haibo , Liu Bifan , Tang Xiongzhuo , Tan Bi’e , Jiang Qian , Yin Yulong
Published: June 2024

Macadamia integrifolia pericarps (MIP) are byproducts of nut production which are rich in natural antioxidant compounds, making them an excellent source for extracting bioactive compounds. However, the antioxidant compounds in MIP are easily oxidized under natural storage conditions, resulting in significant biomass loss and resource wastage. To preserve the potential of MIP to be used as an antioxidant product, we employed cellulase and Limosilactobacillus fermentum ZC529 (L.f ZC529) fermentation and utilized response surface methodology to optimize the fermentation parameters for mitigating the antioxidant loss. Total antioxidant capacity (T-AOC) was used as the response variable. The fermented MIP water extract (FMIPE) was obtained via ultrasound-assisted extraction, and its biological activity was evaluated to optimize the best fermentation conditions. Results indicated that a cellulase dosage of 0.9%, an L.f ZC529 inoculation size of 4 mL/100 g, and a fermentation time of 7 days were the optimal conditions for MIP fermentation. Compared to spontaneous fermentation, these optimal conditions significantly increased the total phenolic and total flavonoid contents (p < 0.05). T-AOC was 160.72% increased by this optimal fermentation (p < 0.05). Additionally, supplementation with varying concentrations of FMIPE (6.25%, 12.5%, and 25%) increased the T-AOC, SOD activity, and GSH content, and reduced MDA levels of the oxidative-stressed Drosophila melanogaster (p < 0.05). Moreover, 12.5% and 25% of FMIPE treatments elevated CAT activity in the Drosophila melanogaster (p < 0.05). The effects of FMIPE on GSH and MDA in Drosophila melanogaster were equivalent to the 0.5% vitamin C (Vc) treatment. In summary, synergistic fermentation using cellulase and L.f ZC529 effectively preserves the antioxidant activity of the MIP, offering a simple, eco-friendly method to promote the utilization of MIP resources.
Research on Validation Method on Retrieval of Atmospheric Temperature and Humidity Profile Using a Microwave Sounder
Authors: He Qiurui , Li Jiaoyang , Zhang Ruiling , Jia Junqi , Guo Xiao
Published: June 2024

The commonly used reference atmospheric profiles for the validation of retrieved atmospheric profiles for microwave sounders have bias compared with real atmospheric profile values, which is detrimental to the validation of the retrieval. Microwave sounder observations are the direct measurements of microwave radiation in atmospheric conditions and are a true representation of the status of the atmosphere. This paper proposed a validation method for the retrieved atmospheric temperature and atmospheric humidity profiles of the satellite-based microwave sounder using its own in-orbit observations. The validation experiments are performed both for the retrievals of the microwave temperature sounder-II (Xi’an Branch, China Academy of Space Technology, Xi’an, China. MWTS-II) and the microwave humidity and temperature sounder (National Space Science Center, Chinese Academy of Sciences, Beijing, China. MWHTS). The validation results show that the retrieved temperature profiles of MWTS-II have higher accuracy compared to the temperature profiles of ERA5 in the atmospheric pressure range of 3–30 hPa, and the accuracy of the rest of the pressure range is comparable between the profiles of ERA5 and the retrieved profiles. And the retrieved temperature profiles of MWHTS have higher accuracy compared to the temperature profiles of ERA5 in the atmospheric pressure level around 50 hPa and lower accuracy in the rest of the pressure levels. In addition, the retrieved humidity profiles of MWHTS have higher accuracy compared to the humidity profiles of ERA5 in the atmospheric pressure range of 350–925 hPa. The proposed validation method for the retrieved atmospheric temperature and atmospheric humidity profiles of MWHTS using its own observations is promising for improving the feasibility and reliability of the validation, and can be a good reference for the application of the satellite in-orbit observations and the optimization of the microwave sounders.
Do Chinese Residents’ Perceptions of Air Pollution Affect Their Evaluation of Central Government Performance? The Moderating Role of Environmental Knowledge
Authors: Shen Yifei , Lu Chuntian , Liu Meng
Published: June 2024

In China, winning the battle for blue skies is a focal point of the central government’s environmental governance efforts. Public evaluations provide validity and legitimacy to the Chinese government’s top-level design for environmental governance. This study utilizes data from two waves of the Chinese General Social Survey (CGSS) conducted in 2013 and 2021, paired with objective air quality data, to conduct a longitudinal analysis of public evaluation of central government environmental governance in China. Furthermore, it explores the relationships between perceived air pollution, objective air quality, environmental knowledge, and public assessment of central government environmental performance. The findings indicate the following: (1) Over the past decade, there has been a noticeable improvement in air quality in China, leading to a significant enhancement in public perception of the central government’s environmental performance. (2) Subjective perceptions of air pollution have a significant negative impact on evaluations of the central government, whereas objective environmental governance measures do not exhibit significant effects. (3) Environmental knowledge plays a negative moderating role in the relationship between perceived air pollution and public assessment of central government environmental performance; individuals with higher levels of environmental knowledge tend to express greater dissatisfaction with the central government’s environmental performance upon perceiving air pollution. These research findings offer valuable insights for informing the formulation of environmental governance policies by the central government of China and provide lessons for other developing and highly polluting countries.
Assessing Carbon Sink Capacity in Coal Mining Areas: A Case Study from Taiyuan City, China
Authors: Chen Fan , Liu Yang , Guo Jinkai , Bai He , Wu Zhitao , Liu Yang , Li Ruijin
Published: June 2024

Climate warming and air pollution are atmospheric environmental problems that have aroused broad concern worldwide. Greenhouse gas emissions are the main cause of global warming. In addition to reducing carbon emissions, increasing carbon sink capacity and improving environmental quality are essential for building green and low-carbon enterprises under carbon peak and carbon neutrality goals. Currently, the research on the methods and application of carbon sink capacity assessment in coal mining enterprises is limited. Given this, this study estimated the carbon absorption, carbon storage, and net ecosystem productivity of a typical coal mining area in Taiyuan City, China, and compared the characteristics and applicability of the three methods. The results showed the following: (1) The total carbon absorption (carbon sink) of the mining area in 2021 was 117.39 t, the primary source of which is forest land. (2) The total carbon storage in the mining area in 2021 was 29,561.96 t. From different land use types, the carbon storage in the mining area mainly came from forest land (27,867.73 t); from the perspective of carbon pool, soil carbon storage (21,970.96 t) had the most significant contribution to the carbon storage of mining areas. (3) The net ecosystem productivity of the mining area in 2021 was 781.97 g/(m2·a), indicating that the ecosystem of the mining area was a carbon sink. (4) The three estimation methods differed in the current case. The estimation method for carbon absorption is the simplest, and the results are the most intuitive. The estimation method for net ecosystem productivity is the most complex. The carbon sink estimation via carbon storage needs to collect two years of data. Enterprises should assess the carbon sink capacity of mining areas based on existing conditions and data. This study proposes methods for estimating carbon sink capacity in mining areas, which have positive practical significance for the low-carbon green development of coal mine enterprises.
Evaluation of the Zenith Tropospheric Delay (ZTD) Derived from VMF3_FC and VMF3_OP Products Based on the CMONOC Data
Authors: Zhang Haoran , Chen Liang , Yang Fei , Ma Jingge , Zhang Junya , Sun Wenyu , Xu Shiqi
Published: June 2024

Prior tropospheric information, especially zenith tropospheric delay (ZTD), is particularly important in GNSS data processing. The two types of ZTD models, those that require and do not require meteorological parameters, are the most commonly used models, whether the non-difference or double-difference mode is applied. To improve the accuracy of prior tropospheric information, the Vienna Mapping Functions (VMFs) data server provides a gridded set of global tropospheric products based on the ray-tracing technique using Numerical Weather Models (NWMs). Note that two types of gridded tropospheric products are provided: the VMF3_OP for the post-processing applications and the VMF3_FC for real-time applications. To explore the accuracy and adaptability of these two grid products, a comprehensive analysis and discussion were conducted in this study using the ZTD data from 255 stations of the Crustal Movement Observation Network of China (CMONOC) as references. The numerical results indicate that both VMF3_FC and VMF3_OP exhibit high accuracy, with RMSE/Bias values of 17.53/2.25 mm and 14.62/2.67 mm, respectively. Both products displayed a temporal trend, with larger RMSE values occurring in summer and smaller values in winter, along with a spatial trend of higher values in the southeast of China and lower values in the northwest of China. Additionally, VMF3_OP demonstrated superior performance to VMF3_FC, with smaller RMSE values for each month and each hour. For the RMSE difference between these two products, 108 stations had a difference of more than 3 mm, and the number of stations with a difference exceeding 1 mm reached 217. Moreover, the difference was more significant in the southeast than in the northwest. This study contributes to the understanding of the differences between the two precision products, aiding in the selection of suitable ZTD products based on specific requirements.
Spatio–Temporal Evolution of Electric Field, Magnetic Field and Thermal Infrared Remote Sensing Associated with the 2021 Mw7.3 Maduo Earthquake in China
Authors: Yang Muping , Zhang Xuemin , Zhong Meijiao , Guo Yufan , Qian Geng , Liu Jiang , Yuan Chao , Li Zihao , Wang Shuting , Zhai Lina , Li Tongxia , Shen Xuhui
Published: June 2024

This study presents the spatio–temporal evolution of the electric and magnetic fields recorded by the China Seismo–Electromagnetic Satellite (CSES) and the thermal infrared remote sensing data observed by the Chinese stationary meteorological satellites Feng Yun–2G (FY–2G) associated with the 2021 Mw7.3 Maduo earthquake. Specifically, we analyzed the power spectrum density (PSD) data of the electric field in the extremely low frequency (ELF) band, the geomagnetic east–west vector data, and the temperature of brightness blackbody (TBB) data to investigate the spatio–temporal evolution characteristics under quiet space weather conditions (Dst > −30 nT and Kp < 3). Results showed that (1) the TBB radiation began to increase notably along the northern fault of the epicenter ~1.5 months prior to the occurrence of the earthquake. It achieved its maximum intensity on 17 May, and the earthquake occurred as the anomalies decreased. (2) The PSD in the 371 Hz–500 Hz and 700 Hz–871 Hz bands exhibited anomaly perturbations near the epicenter and its magnetic conjugate area on May 17, with particularly notable perturbations observed in the latter. The anomaly perturbations began to occur ~1 month before the earthquake, and the earthquake occurred as the anomalies decreased. (3) Both the magnetic –east–west component vector data and the ion velocity Vx data exhibited anomaly perturbations near the epicenter and the magnetic conjugate area on 11 May and 16 May. (4) The anomaly perturbations in the thermal infrared TBB data, CSES electric field, and magnetic field data all occurred within a consistent perturbation time period and spatial proximity. We also conducted an investigation into the timing, location, and potential causes of the anomaly perturbations using the Vx ion velocity data with magnetic field –east–west component vector data, as well as the horizontal –north–south and vertical component PSD data of the electric field with the magnetic field –east–west component vector data. There may be both chemical and electromagnetic wave propagation models for the “lithosphere—atmosphere—ionosphere” coupling (LAIC) mechanism of the Maduo earthquake.
Finite Element Analysis of Cervical Spine Kinematic Response during Ejection Utilising a Hill-Type Dynamic Muscle Model
Authors: Gong Yikang , Cheng Zhenghan , Teo Ee-Chon , Gu Yaodong
Published: June 2024

To determine the impact of active muscle on the dynamic response of a pilot’s neck during simulated emergency ejection, a detailed three-dimensional (3D) cervical spine (C0–T1) finite element (FE) model integrated with active muscles was constructed. Based on the Hill-type model characterising the muscle force activation mechanics, 13 major neck muscles were modelled. The active force generated by each muscle was simulated as functions of (i) active state (Na), (ii) velocity (Fv(v)), and (iii) length (FL(L)). An acceleration-time profile with an initial acceleration rate of 125 G·s−1 in the 0–80 ms period, reaching peak acceleration of 10 G, then kept constant for a further 70 ms, was applied. The rotational angles of each cervical segment under these ejection conditions were compared with those without muscles and with passive muscles derived from the previous study. Similar trends of segmental rotation were observed with S- and C-curvature of the cervical spine in the 150 ms span analysed. With active muscles, the flexion motion of the C0–C2 segments exhibited higher magnitudes of rotation compared to those without muscle and passive muscle models. The flexion motion increased rapidly and peaked at about 95–105 ms, then decreased rapidly to a lower magnitude. Lower C2–T1 segments exhibited less variation in flexion and extension motions. Overall, during emergency ejections, active muscle activities effectively reduce the variability in rotational angles across cervical segments, except C0–C2 segments in the 60–120 ms period. The role of the active state dynamics of the muscles was crucial to the magnitude of the muscle forces demonstrated. This indicates that it is crucial for pilots to consciously contract their muscles before ejection to prevent cervical spine injuries.
Uncovering Interoceptive Human Insular Lobe Function through Intraoperative Cortical Stimulation—A Review
Authors: Zinn Pascal O. , Habib Ahmed , Deng Hansen , Gecici Neslihan Nisa , Elidrissy Hayat , Alami Idrissi Yassine , Amjadzadeh Mohammadreza , Sherry Natalie Sandel
Published: June 2024

The insular cortex, a critical hub in the brain’s sensory, cognitive, and emotional networks, remains an intriguing subject of study. In this article, we discuss its intricate functional neuroanatomy, emphasizing its pivotal role in processing olfactory information. Through concise exploration, we delve into the insula’s diverse connectivity and its involvement in sensory integration, particularly in olfaction. Stimulation studies in humans reveal compelling insights into the insula’s contribution to the perception of smell, hinting at its broader implications for cognitive processing. Additionally, we explore an avenue of research in which studying olfactory processing via insular stimulation could unravel higher-level cognitive processes. This innovative approach could help give a fresh perspective on the interplay between sensory and cognitive domains, offering valuable insights into the neural mechanisms underlying cognition and emotion. In conclusion, future research efforts should emphasize a multidisciplinary approach, combining advanced imaging and surgical techniques to explore the intricate functions of the human insula. Moreover, awake craniotomies could offer a unique opportunity for real-time observation, shedding light on its neural circuitry and contributions to higher-order brain functions. Furthermore, olfaction’s direct cortical projection enables precise exploration of insular function, promising insights into cognitive and emotional processes. This multifaceted approach will deepen our understanding of the insular cortex and its significance in human cognition and emotion.
Have I Been Touched? Subjective and Objective Aspects of Tactile Awareness
Authors: Cirillo Emanuele , Zavattaro Claudio , Gammeri Roberto , Serra Hilary , Ricci Raffaella , Berti Anna
Published: June 2024

Somatosensory tactile experience is a key aspect of our interaction with the environment. It is involved in object manipulation, in the planning and control of actions and, in its affective components, in the relationships with other individuals. It is also a foundational component of body awareness. An intriguing aspect of sensory perception in general and tactile perception in particular is the way in which stimulation comes to consciousness. Indeed, although being aware of something seems a rather self-evident and monolithic aspect of our mental states, sensory awareness may be in fact modulated by many different processes that impact on the mere stimulation of the skin, including the way in which we perceive our bodies as belonging to us. In this review, we first took into consideration the pathological conditions of absence of phenomenal experience of touch, in the presence of implicit processing, as initial models for understanding the neural bases of conscious tactile experience. Subsequently, we discussed cases of tactile illusions both in normal subjects and in brain-damaged patients which help to understand which high-order processes impact tactile awareness. Finally, we discussed the observations reported in the review in light of some influential models of touch and body representation.
The Complex Relationship between Sleep and Cognitive Reserve: A Narrative Review Based on Human Studies
Authors: Balsamo Francesca , Berretta Erica , Meneo Debora , Baglioni Chiara , Gelfo Francesca
Published: June 2024

Sleep and brain/cognitive/neural reserve significantly impact well-being and cognition throughout life. This review aims to explore the intricate relationship between such factors, with reference to their effects on human cognitive functions. The specific goal is to understand the bidirectional influence that sleep and reserve exert on each other. Up to 6 February 2024, a methodical search of the literature was conducted using the PubMed database with terms related to brain, cognitive or neural reserve, and healthy or disturbed sleep. Based on the inclusion criteria, 11 articles were selected and analyzed for this review. The articles focus almost exclusively on cognitive reserve, with no explicit connection between sleep and brain or neural reserve. The results evidence sleep’s role as a builder of cognitive reserve and cognitive reserve’s role as a moderator in the effects of physiological and pathological sleep on cognitive functions. In conclusion, the findings of the present review support the notion that both sleep and cognitive reserve are critical factors in cognitive functioning. Deepening comprehension of the interactions between them is essential for devising strategies to enhance brain health and resilience against age- and pathology-related conditions.
Designing for a Circular Economy in the Architecture, Engineering, and Construction Industry: Insights from Italy
Authors: Alhawamdeh Mahmoud , Lee Angela , Saad Ali
Published: June 2024

Resource consumption in the construction industry is expected to increase globally in the coming years. Additionally, construction and demolition waste (CDW) remains a significant priority within numerous global policies due to its vast volume and the inefficiencies in its management. This situation results in substantial environmental repercussions, primarily due to the low rates of material recovery in the manufacturing processes for new building materials. In response, the concept of the circular economy (CE) emerges as a promising solution across various sectors. CE promotes more resource- and energy-efficient practices, reducing waste generation and mitigating the environmental impacts associated with product life cycles while also unlocking potential economic opportunities. The primary aim of this study is to identify and assess the design practices influencing the adoption of CE principles within the Italian architectural, engineering, and construction (AEC) sector. The study’s main contribution lies in a survey of 77 Italian designers to explore the core strategies driving the development of comprehensive circular approaches. This investigation seeks to understand the constraints and opportunities for CE implementation. The findings will assist in decision-making, inform policy, promote literacy around the CE topic, enable new quality standards, and serve as a baseline reference for businesses regarding sustainability investment indexes and markets.