bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Osteoporosis Pharmacotherapy on Functional Outcomes after Ischemic Stroke
Authors: Sohn Jong-Hee , Kim Chulho , Kim Yerim , Park So Young , Lee Sang-Hwa
Published: July 2023

This study evaluated whether osteoporosis pharmacotherapy (OPT) affected functional outcomes in acute ischemic stroke patients with osteoporosis. Using a single-center registry database, we consecutively registered acute ischemic stroke patients between May 2016 and December 2020. All patients older than 55 years underwent routine bone densitometry within 7 days of stroke onset. OPT prescription was confirmed by reviewing medical records. We classified the patients into OPT and no OPT groups. We performed propensity score matching (PSM) to overcome the imbalance in multiple covariates between the two groups. We investigated whether OPT affected 1-year functional outcomes by multivariate analysis using a PSM cohort. Among 1307 consecutively registered acute ischemic stroke patients, 381 patients were enrolled in this study, of whom 134 (35.2%) were prescribed OPT at discharge, which was maintained for 1 year. In a multivariate analysis using a PSM cohort, the OPT group had a lower risk of dependency (odds ratio [OR], 0.52; 95% confidence interval [CI], 0.27–0.996) and poor functional outcome at 1 year (OR, 0.24; 95% CI, 0.10–0.57). The OPT group also had increased chance of late functional improvement (OR, 6.16; 95% CI, 1.12–33.79). This study showed that OPT could reduce dependency and poor functional outcomes and increase the chance of improving functional outcomes at 3 months and 1 year after ischemic stroke onset, and these findings could be helpful for improving functional outcomes and bone health after ischemic stroke.
Endoscopic Conservative Treatment of Upper Urinary Tract Urothelial Carcinoma with a Thulium Laser: A Systematic Review
Authors: Candela Luigi , Ventimiglia Eugenio , Solano Catalina , Chicaud Marie , Kutchukian Stessy , Panthier Frederic , Corrales Mariela , Villa Luca , Briganti Alberto , Montorsi Francesco , Salonia Andrea , Doizi Steeve , Traxer Olivier
Published: July 2023

Introduction: Thulium lasers (TLs), namely the Thulium fiber laser (TFL) and the Thulium:YAG (Tm:YAG), are being increasingly adopted for the conservative treatment of upper urinary tract urothelial carcinoma (UTUC). However, to date, the real clinical impact of TLs on UTUC management remains not well-characterized. We performed a review of the literature to summarize the current evidence on TLs for UTUC treatment. Materials and Methods: We performed a systematic review in January 2023 using the Embase and Medline online databases, according to the PRISMA recommendations and using the PICO criteria. Outcomes of interest were: (i) to assess the safety and feasibility of TLs in the treatment of UTUC, and (ii) to evaluate the oncological outcomes in terms of tumor recurrence and conservative treatment failure. Moreover, we described TL characteristics and its interaction with soft tissue. Results: a total of 458 articles were screened, and six full texts including 273 patients were identified. All the included studies were retrospective series. Mean patient age ranged from 66 to 73 years. The indication of a conservative treatment was elective and imperative in 21.7–85% and 15–76% of cases, respectively. Laser power settings varied from 5 to 50 W. No intraoperative complications were reported, and all the procedures were successfully performed. The tumor recurrence rate was 17.7–44%, and the indication to radical nephroureterectomy was 3.7–44% during a follow-up of 6–50 months. Most of the postoperative complications were mild and transient, and ureteral strictures were reported in two studies. Major limitations were the retrospective nature of the studies, the small sample sizes, and the short follow-up. Conclusions: TL is an effective and safe technology for endoscopic UTUC treatment. However, current available literature lacks prospective and multicentric studies with large population sizes and long-term follow-up.
Anatomical and Surgical Implications of the Usage of Bichat Fat Pad in Oroantral Communication, Maxillary, Palatal, and Related Surgeries—Narrative Review
Authors: Nelke Kamil , Morawska Alicja , Błaszczyk Bartłomiej , Janeczek Maciej , Pasicka Edyta , Łukaszewski Marceli , Żak Krzysztof , Dobrzyński Maciej
Published: July 2023

The buccal fat pad, also called the Bichat’s fat pad (BFP), is an encapsulated fat mass located in the cheek. This type of specialized fat mass can be used both as a pedicular or free graft in various surgeries and approaches. Due to its easy access from the oral cavity approach, it is commonly used for oroantral and palatal fistula closure. The knowledge of its anatomy and surrounding tissues plays a role in its mobilization and suturing onto the desired defect in the palatal or maxillary region. The BFP is mostly associated with the primary approach used for a fistula or bone surgery. Alternatively, the procedure can be performed with a single approach incision, which does not compromise the appearance or the function of the operating or adjacent areas. The most important inclusion criteria for BFP usage and surgical limitations are highlighted. The BFP is used for multiple purposes in reconstructive and oncology surgery and also has its use in esthetic and facial contouring procedures. The amount, volume, and shape of the BFP are mostly associated with the scope of their usage. The aim of the following narrative review is to present the surgical and anatomical implications of fat pads in maxillary and palatal surgeries.
Emerging Therapies for Antibody-Mediated Rejection in Kidney Transplantation
Authors: Abuazzam Farah , Dubrawka Casey , Abdulhadi Tarek , Amurao Gwendolyn , Alrata Louai , Yaseen Alsabbagh Dema , Alomar Omar , Alhamad Tarek
Published: July 2023

Despite the advances in immunosuppressive medications, antibody-mediated rejection (AMR) continues to be a major cause of kidney allograft failure and remains a barrier to improving long-term allograft survival. Recently, there have been significant advances in the understanding of the pathophysiological process of AMR, along with the development of new therapeutic options. Additionally, surveillance protocols with donor-derived cell-free DNA and gene profile testing have been established, leading to the early detection of AMR. A multitude of clinical trials are ongoing, opening numerous opportunities for improving outcome in kidney transplant recipients. In this brief review, we discuss the emerging therapies for managing both active and chronic active AMR and highlight the ongoing clinical trials.
Right Transcephalic Ventriculo-Subclavian Shunt in the Surgical Treatment of Hydrocephalus—An Original Procedure for Drainage of Cerebrospinal Fluid into the Venous System
Authors: Liţescu Mircea , Cristian Daniel Alin , Coman Violeta Elena , Erchid Anwar , Pleşea Iancu Emil , Bordianu Anca , Lupaşcu-Ursulescu Corina Veronica , Florea Costin George , Coman Ionuţ Simion , Grigorean Valentin Titus
Published: July 2023

The objectives of this article are to present an original surgical procedure for the temporary or definitive resolution of hydrocephalus, in the case of repeated failure of standard treatment techniques, and to present a case that was resolved using this surgical technique. Materials and methods: We present the case of a 20-year-old male patient with congenital hydrocephalus who underwent a number of 39 shunt revisions, given the repetitive dysfunctions of various techniques (ventriculo-peritoneal shunt, ventriculo-cardiac shunt). The patient was evaluated with the ventricular catheter externalized at the distal end and it was necessary to find an emergency surgical solution, considering the imminent risk of meningitis. The patient was also associated with the diagnosis of acute lithiasic cholecystitis. Results and discussions: The final chosen solution, right ventriculo-venous drainage using the cephalic vein, was a temporary surgical solution, but there are signs that this procedure can provide long-term ventricular drainage. Conclusions: Transcephalic ventriculo-subclavian drainage represents an alternative technical option, which can be used when established options become ineffective.
Ice Composites Strengthened by Organic and Inorganic Nanoparticles
Authors: Golovin Yuri I. , Samodurov Alexander A. , Tyurin Alexander I. , Rodaev Vyacheslav V. , Golovin Dmitry Yu. , Vasyukov Vladimir M. , Razlivalova Svetlana S. , Buznik Vyacheslav M.
Published: July 2023

Extensive development of the Arctic, rich in various natural resources, requires a lot of cheap ecologically safe construction materials preferably using local renewable sources. Ice being easily available in regions with a cold climate meets all the above criteria but has low strength, high fragility and a tendency to creep. Known strengthening techniques using macroscopic and microfiber additives have many drawbacks. This paper describes a new approach to ice strengthening by adding organic or inorganic 50–100 nm nanoparticles (NPs) to the water to be frozen and presents experimental results and possible mechanisms analysis of polycrystalline ice strengthening using NPs. Cellulose and silica NPs have been tested as typical representatives of such additives. Both are hydrophilic, ecologically safe, widespread, cheap and can be produced from local renewable raw materials. It is shown that the addition of 0.01 to 5 wt.% of NPs results in a reduction of ice composite average grain size by a factor of 5.3 ± 0.7 and an increase of its compression strength by a factor of 2.5 ± 0.3. The highest sensitivity of ice strength to NPs concentration is between 0.1 and 1 wt.%. In this concentration range, the increase in strength is reversely proportional to the square root of the average grain size. Experimental data fit Griffith’s relation better than Hall–Petch, so ice strength is limited by cracks with the length proportional to average grain size.
Carbonization of Zr-Loaded Thiourea-Functionalized Styrene-Divinylbenzene Copolymers: An Easy Way to Synthesize Nano-ZrO2@C and Nano-(ZrC, ZrO2)@C Composites
Authors: Martiz Alejandro , Károly Zoltán , Bereczki Laura , Trif László , Farkas Attila , Menyhárd Alfréd , Kótai László
Published: July 2023

Thermal processing of Zr-loaded ion-exchangers is a facile route to synthetize (ZrO2, ZrC)@C composites. In the present paper, furnace and RF-thermal plasma processing of ZrOCl2 loaded thiourea-functionalized styrene-divinylbenzene copolymer was investigated and led to composites containing ZrO2 and ZrC. Different ZrO2@C composites were formed between 1000 and 1400 °C in 2 h, whereas the composite containing ZrC was created at 1400 °C in 8 h. The ratio of ZrO2/ZrC, the prevailing ZrO2 modifications, and the crystallite sizes strongly depend on the synthesis conditions. The ZrC-containing composites formed only at 1400 °C in 8 h and by the plasma treatment of the ZrO2@C sample prepared in the furnace, resulting in 8 and 16% ZrC content, with 44 and 41 nm ZrC crystallite sizes, respectively. The ZrO2-containing composites (tetragonal, monoclinic, and cubic modifications with 65–88 nm ZrO2 crystallite sizes and 15–43 m2/g BET surface areas) formed in a tube furnace between 1000 and 1400 °C in 2 h. All ZrO2@C composites had both amorphous carbon and graphite, and their ratio is temperature dependent. The carbonaceous compounds were characterized by Raman spectroscopy with analysis of the G and D band intensities. XPS studies showed the surface oxidation of ZrC.
Multifunctional Graphene–Polymer Nanocomposite Sensors Formed by One-Step In Situ Shear Exfoliation of Graphite
Authors: Ashraf Ali , Chang Elizabeth , Rahman Md Ashiqur , Ghosh Dipannita , Islam Nazmul , Lynch-Branzoi Jennifer K.
Published: July 2023

Graphene nanocomposites are a promising class of advanced materials for sensing applications; yet, their commercialization is hindered due to impurity incorporation during fabrication and high costs. The aim of this work is to prepare graphene–polysulfone (G−PSU) and graphene–polyvinylidene fluoride (G−PVDF) nanocomposites that perform as multifunctional sensors and are formed using a one-step, in situ exfoliation process whereby graphite is exfoliated into graphene nanoflakes (GNFs) directly within the polymer. This low-cost method creates a nanocomposite while avoiding impurity exposure since the raw materials used in the in situ shear exfoliation process are graphite and polymers. The morphology, structure, thermal properties, and flexural properties were determined for G−PSU and G−PVDF nanocomposites, as well as the electromechanical sensor capability during cyclic flexural loading, temperature sensor testing while heating and cooling, and electrochemical sensor capability to detect dopamine while sensing data wirelessly. G−PSU and G−PVDF nanocomposites show superior mechanical characteristics (gauge factor around 27 and significantly enhanced modulus), thermal characteristics (stability up to 500 °C and 170 °C for G−PSU and G−PVDF, respectively), electrical characteristics (0.1 S/m and 1 S/m conductivity for G−PSU and G−PVDF, respectively), and distinguished resonant peaks for wireless sensing (~212 MHz and ~429 MHz). These uniquely formed G−PMC nanocomposites are promising candidates as strain sensors for structural health monitoring, as temperature sensors for use in automobiles and aerospace applications, and as electrochemical sensors for health care and disease diagnostics.
Fast Compressed Sensing of 3D Radial T1 Mapping with Different Sparse and Low-Rank Models
Authors: Paajanen Antti , Hanhela Matti , Hänninen Nina , Nykänen Olli , Kolehmainen Ville , Nissi Mikko J.
Published: July 2023

Knowledge of the relative performance of the well-known sparse and low-rank compressed sensing models with 3D radial quantitative magnetic resonance imaging acquisitions is limited. We use 3D radial T1 relaxation time mapping data to compare the total variation, low-rank, and Huber penalty function approaches to regularization to provide insights into the relative performance of these image reconstruction models. Simulation and ex vivo specimen data were used to determine the best compressed sensing model as measured by normalized root mean squared error and structural similarity index. The large-scale compressed sensing models were solved by combining a GPU implementation of a preconditioned primal-dual proximal splitting algorithm to provide high-quality T1 maps within a feasible computation time. The model combining spatial total variation and locally low-rank regularization yielded the best performance, followed closely by the model combining spatial and contrast dimension total variation. Computation times ranged from 2 to 113 min, with the low-rank approaches taking the most time. The differences between the compressed sensing models are not necessarily large, but the overall performance is heavily dependent on the imaged object.
A Study on the Hydrodynamics and Coupling Effects of the Multibody Floating Photovoltaic (FPV) Concept
Authors: Zhang Fan , Shi Wei , Wang Qingqing
Published: July 2023

Floating photovoltaics (FPVs) have been developed rapidly in the past few years and will gradually become the “third pillar” of the photovoltaic industry. To better understand the performance of FPV floaters, this paper provides an in-depth study on the hydrodynamics of a single FPV module and the coupling effects of multiple modules. The results show that a conventional frequency domain approach, which includes both panel and Morison models, may not necessarily provide realistic results. Even after adding an additional damping matrix for the floaters based on empirical values from the oil and gas (O&G) industry, and a free surface damping model between the pontoons, the responses were still not convincing. Therefore, a nonlinear time-domain hydrodynamic solver was introduced. Further studies and comparisons were performed to understand the behavior of the module, and some updated damping coefficients were summarized. Thereafter, a multibody hydrodynamic model was built to check the coupling effects. With the additional damping surface on the gap surface among the modules, some attempts were made to derive reasonable results, when the model test was not available. Preliminary studies of both a scaled-down system (with 9 modules and mooring lines) and a full-scale system (with 90 modules, buoys, and mooring systems) were also investigated, and some initial results were demonstrated.
Light-Induced Changes in Secondary Metabolite Production of Trichoderma atroviride
Authors: Missbach Kristina , Flatschacher Daniel , Bueschl Christoph , Samson Jonathan Matthew , Leibetseder Stefan , Marchetti-Deschmann Martina , Zeilinger Susanne , Schuhmacher Rainer
Published: July 2023

Many studies aim at maximizing fungal secondary metabolite production but the influence of light during cultivation has often been neglected. Here, we combined an untargeted isotope-assisted liquid chromatography–high-resolution mass spectrometry-based metabolomics approach with standardized cultivation of Trichoderma atroviride under three defined light regimes (darkness (PD), reduced light (RL) exposure, and 12/12 h light/dark cycle (LD)) to systematically determine the effect of light on secondary metabolite production. Comparative analyses revealed a similar metabolite profile upon cultivation in PD and RL, whereas LD treatment had an inhibiting effect on both the number and abundance of metabolites. Additionally, the spatial distribution of the detected metabolites for PD and RL was analyzed. From the more than 500 detected metabolites, only 25 were exclusively produced upon fungal growth in darkness and 85 were significantly more abundant in darkness. The majority were detected under both cultivation conditions and annotation revealed a cluster of substances whose production followed the pattern observed for the well-known T. atroviride metabolite 6-pentyl-alpha-pyrone. We conclude that cultivation of T. atroviride under RL can be used to maximize secondary metabolite production.
Interactions of EGFR/PTEN/mTOR-Pathway Activation and Estrogen Receptor Expression in Cervical Cancer
Authors: Bartl Thomas , Grimm Christoph , Mader Robert M. , Zielinski Christoph , Prager Gerald , Unseld Matthias , Herac-Kornauth Merima
Published: July 2023

(1) Objective: Late-line chemotherapy rechallenge in recurrent cervical cancer is associated with modest therapy response but significant side effects. As mTOR pathways modulate cellular growth via estrogen receptor (ER) signaling and combined mTOR and ER inhibition previously demonstrated survival benefits in breast cancer, this exploratory study evaluates mTOR pathway and ER expression interactions in a preclinical cervical cancer model. (2) Methods: Immunostaining of a 126-tumor core tissue microarray was performed to assess phosphorylated-mTOR and ER expression. To identify tumor subsets with different clinical behavior, expression results were matched with clinicopathologic patient characteristics, and both univariate and multivariable survival statistics were performed. (3) Results: phosphorylated-mTOR correlates with ER (r = 0.309, p < 0.001) and loss of PTEN expression (r = −2.09, p = 0.022) in tumor samples across stages but not in matched negative controls. Positive ER expression is observed significantly more often in phosphorylated-mTOR positive samples (30.0% vs. 6.3%, p = 0.001). In the subgroup of phosphorylated-mTOR positive tumors (n = 60), ER expression is associated with improved survival (p = 0.040). (4) Conclusion: ER expression appears closely intertwined with EGFR/PTEN/mTOR-pathway activation and seems to define a subgroup with clinically distinct behavior. Considering limited therapeutic options in recurrent cervical cancer, further validation of combined mTOR and ER inhibition in selected patients could appear promising.
Longitudinal Analysis of Pulmonary Function Impairment One Year Post-COVID-19: A Single-Center Study
Authors: Suppini Noemi , Fira-Mladinescu Ovidiu , Traila Daniel , Motofelea Alexandru Catalin , Marc Monica Steluta , Manolescu Diana , Vastag Emanuela , Maganti Ram Kiran , Oancea Cristian
Published: July 2023

Persistent pulmonary impairment post-COVID-19 has been reported, albeit variably. This single-center observational study aims to longitudinally evaluate pulmonary function in 140 COVID-19 survivors one year after recovery, assessing associations with disease severity and pre-existing lung conditions. Participants aged 18 and older, with confirmed SARS-CoV-2 infection, were evaluated using spirometry and Diffusion Capacity of Lungs for Carbon Monoxide (DLCO) tests. Pulmonary function parameters like Forced Expiratory Volume at 1 s (FEV1), Forced Vital Capacity (FVC), and Total Lung Capacity (TLC) were measured. Participants were stratified by age, gender, body mass index, smoking status, and lung damage severity via computed tomography (CT). The cohort consisted of mostly males (58.6%), with a mean age of 53.8 years and body mass index of 24.9 kg/m2. Post-COVID fibrosis was seen in 22.7%, 27.3%, and 51.9% of mild, moderate, and severe disease patients, respectively (p = 0.003). FVC significantly reduced with disease severity (p < 0.001), while FEV1, FEF25-75, and DLCO showed a non-significant downward trend. FEV1/FVC ratio increased with disease severity (p = 0.033), and TLC and RV significantly declined (p = 0.023 and p = 0.003, respectively). A one-year follow-up indicated a non-significant change in FVC, FEV1, FEV1/FVC ratio, FEF25-75, and RV compared with the 40-day measurement, but it revealed significant improvements in DLCO and TLC (p = 0.010). There were significant mean increases in FVC, FEV1, DLCO, TLC, and RV across all disease severities over one year. They were most pronounced in the patients with a history of severe COVID-19, who had a better recovery over one year, compared with the mild and moderate COVID-19 patients whose lung function almost normalized. One year after the SARS-CoV-2 infection, we observed a significant association between disease severity and post-COVID fibrotic changes. Though some lung function parameters remained stable over the year, significant improvements were noted in DLCO and TLC. Particularly, individuals with severe disease showed substantial recovery in lung function, indicating the potential reversibility of COVID-19-related pulmonary damage.
Visualizing the Landscape of Green Gentrification: A Bibliometric Analysis and Future Directions
Authors: Gou Feicui , Zhai Wenya , Wang Zilin
Published: July 2023

Green gentrification has emerged as a critical and dynamic research field in environmental justice and gentrification. Few studies have systematically reviewed the current state of green gentrification literature. This paper adopts a CiteSpace-based bibliometric approach to quantitatively and qualitatively analyze 186 publications from the Web of Science database from 2009 to 2022. The objective is to assess the fundamental characteristics, identify research hotspots, themes, frontiers, and future trends in green gentrification, and present a knowledge map. The results reveal the following: (1) Green gentrification research has moved through the preparation, primary, and prosperity stages and, since 2014, has experienced exponential growth while maintaining a robust upward trend. Knowledge of green gentrification is primarily drawn from urban studies, environmental research, geography, and interdisciplinary trends that are increasingly apparent. Although North America and Europe have produced the most research outcomes, the number of studies from South America, Asia, and Australia is rising. (2) Research hotspots initially comprised the conceptualization and theoretical framework of green gentrification and then shifted to empirical studies that identify green gentrification and its social–spatial effects. The current research focus is on the mechanisms and characteristics of green gentrification. (3) Five key themes were identified, including conceptualizing green gentrification, operationalizing green gentrification for local applicability, social–spatial effects, exploring explanatory frameworks or models, and strategic response to green gentrification. (4) Four potential future directions for green gentrification research in the context of three current research trends were proposed: broadening the scope of non-North American case studies, advancing interdisciplinary theoretical innovation, expanding the analysis of green gentrification characteristics, and exploring demand-side drivers of green gentrification. This study enhances the understanding of existing knowledge in green gentrification-related research and provides a reference for future theoretical and empirical studies.
Bilingualism in Brazil: An Examination of Its Effect on the Formation of Individual Identities
Authors: Arnaiz-Castro Patricia , Espejo-Mohedano Roberto
Published: July 2023

The 21st century has witnessed a substantial increase in research focused on the benefits of bilingualism for individuals. The aspects that have received the most attention have been executive functions. And communication skills. Less is known, however, about the noncognitive and nonlinguistic aspects of bilingualism. Personality psychologists claim that personality is the result of a combination of nature-related and nurture-related factors, but the latter have not been sufficiently addressed in studies on bilingualism. Thus, to bridge this gap, the present contribution pursues this line of inquiry by adopting a quantitative approach to the examination of the self-perceived mobility, employability, and intercultural competence of participants who studied in a bilingual education program. A total of 835 respondents living in Brazil completed an online questionnaire. Mann–Whitney U and Wilcoxon W tests showed higher scores for bilingual graduates than for their nonbilingual counterparts on the three subscales. Correlation analyses revealed moderate-to-high positive correlations between bilingual graduates’ perception of their way of being and their way of life on the one hand, and among their self-perceived mobility, employability, and intercultural competence on the other. Likewise, the analyses showed statistically significant positive correlations between being proficient in more than one foreign language and the dimension of mobility. Our findings illustrate the influence that bilingualism/multilingualism exerts on factors that determine everyday life and corroborate and expand the research conducted in this strand. Avenues for further related research are discussed.
Importance of Pharmacogenetics and Drug–Drug Interactions in a Kidney Transplanted Patient
Authors: Concha Julia , Sangüesa Estela , Saez-Benito Ana M. , Aznar Ignacio , Berenguer Nuria , Saez-Benito Loreto , Ribate M. Pilar , García Cristina B.
Published: July 2023

Tacrolimus (TAC) is a narrow-therapeutic-range immunosuppressant drug used after organ transplantation. A therapeutic failure is possible if drug levels are not within the therapeutic range after the first year of treatment. Pharmacogenetic variants and drug–drug interactions (DDIs) are involved. We describe a patient case of a young man (16 years old) with a renal transplant receiving therapy including TAC, mycophenolic acid (MFA), prednisone and omeprazole for prophylaxis of gastric and duodenal ulceration. The patient showed great fluctuation in TAC blood concentration/oral dose ratio, as well as pharmacotherapy adverse effects (AEs) and frequent diarrhea episodes. Additionally, decreased kidney function was found. A pharmacotherapeutic follow-up, including pharmacogenetic analysis, was carried out. The selection of the genes studied was based on the previous literature (CYP3A5, CYP3A4, POR, ABCB1, PXR and CYP2C19). A drug interaction with omeprazole was reported and the nephrologist switched to rabeprazole. A lower TAC concentration/dose ratio was achieved, and the patient’s condition improved. In addition, the TTT haplotype of ATP Binding Cassette Subfamily B member 1 (ABCB1) and Pregnane X Receptor (PXR) gene variants seemed to affect TAC pharmacotherapy in the studied patient and could explain the occurrence of long-term adverse effects post-transplantation. These findings suggest that polymorphic variants and co-treatments must be considered in order to achieve the effectiveness of the immunosuppressive therapy with TAC, especially when polymedicated patients are involved. Moreover, pharmacogenetics could influence the drug concentration at the cellular level, both in lymphocyte and in renal tissue, and should be explored in future studies.
In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
Authors: Seisenov Bolat , Duimbayev Dulat , Kazhgaliyev Nurlybay , Abdrakhmanov Talgat , Tegza Alexandra , Abeldinov Rustem , Burambayeva Nadezhda , Temirzhanova Alma , Tegza Ivan , Kemeshev Zhomart , Zhanabayev Assylbek , Akhmetbekov Nurbolat , Aisin Marat , Zhugunissov Kuandyk , Issimov Arman
Published: July 2023

In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (p ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms.
Extracellular Vesicles and Cancer Multidrug Resistance: Undesirable Intercellular Messengers?
Authors: Bucci-Muñoz María , Gola Aldana Magalí , Rigalli Juan Pablo , Ceballos María Paula , Ruiz María Laura
Published: July 2023

Cancer multidrug resistance (MDR) is one of the main mechanisms contributing to therapy failure and mortality. Overexpression of drug transporters of the ABC family (ATP-binding cassette) is a major cause of MDR. Extracellular vesicles (EVs) are nanoparticles released by most cells of the organism involved in cell–cell communication. Their cargo mainly comprises, proteins, nucleic acids, and lipids, which are transferred from a donor cell to a target cell and lead to phenotypical changes. In this article, we review the scientific evidence addressing the regulation of ABC transporters by EV-mediated cell–cell communication. MDR transfer from drug-resistant to drug-sensitive cells has been identified in several tumor entities. This was attributed, in some cases, to the direct shuttle of transporter molecules or its coding mRNA between cells. Also, EV-mediated transport of regulatory proteins (e.g., transcription factors) and noncoding RNAs have been indicated to induce MDR. Conversely, the transfer of a drug-sensitive phenotype via EVs has also been reported. Additionally, interactions between non-tumor cells and the tumor cells with an impact on MDR are presented. Finally, we highlight uninvestigated aspects and possible approaches to exploiting this knowledge toward the identification of druggable processes and molecules and, ultimately, the development of novel therapeutic strategies.
A Comparison of Truck Driver Pre-Employment Hair and Urine Drug Test Efficacy
Authors: Li Ming , Voss M. Douglas , Cangelosi Joseph D.
Published: July 2023

Background: To help ensure roadway safety, the federal government mandates trucking companies to conduct pre-employment urine drug tests before allowing drivers to operate a commercial motor vehicle. Unfortunately, urine testing has a short detection window and is easily thwarted, leading some carriers to employ hair testing. Methods: t-tests were utilized to compare hair and urine pre-employment drug test results provided by seven large U.S. trucking companies. Results: results indicate that hair’s positivity rate is statistically greater than urine for each examined drug and across all drugs combined. Conclusions: This paper is the only supply chain work of which we are aware that assesses the statistical differences between hair and urine testing positivity rates. Results support hair testing’s increased ability to prevent lifestyle drug users from operating commercial motor vehicles and should be considered by public policy makers considering whether hair testing results should be allowed into the Drug and Alcohol Clearinghouse.
Wear Resistance of In Situ NbC-Reinforced Laser Cladding Ni45 Coatings
Authors: Liu Yingpeng , Wang Kaiming , Fu Hanguang , Zong Bin , Zhang Jiguang
Published: July 2023

In situ NbC-reinforced laser cladding Ni45 coatings have the advantages of high bond strengths, low dilution rates, small heat-affected zones and good wear resistance and have broad application prospects in the field of surface strengthening and repair of workpieces such as automotive molds and engine turbines. Previous studies have mostly used pure niobium powder for in situ synthesis to prepare Ni-based NbC coatings with a high production cost. In this paper, NbC was successfully synthesized in situ in Ni45 powder using inexpensive FeNb65 and Cr3C2. The prepared coating has a uniform microstructure and excellent wear resistance, and the reinforced phases are mainly NbC and Cr23C6. Coating 4# with 25 wt.% FeNb65 + Cr3C2 has the highest microhardness of 776.3HV0.2, about 1.45 times that of the Ni45 coating, and its wear resistance is 36.36 min/mg, about 60.6 times that of the Cr12MoV steel base material and about 23.76 times that of the Ni45 coating.
Evaluation of Fluidity and Strength of High-Early-Strength Cement-Based Repair Materials by Adding SB Latex and Wollastonite Mineral Fibers
Authors: Choo Yeon-Jae , Koo Jae-Hyuk , Lee Su-Jin , Park Chan-Gi
Published: July 2023

Concrete structures often fail to perform their original functions due to problems such as deterioration and damage over time. Therefore, various repair materials have been studied to maintain deteriorated concrete structures. This study experimentally investigated the mechanical properties of high-early-strength cement-based repair materials for spraying. For spraying, the cement-based materials should have adoptable fluidity and strength: 200 ± 100 mm for flow; 20 MPa at 24 h and 40 MPa at 28 days for compressive strength, and 8 MPa at 28 days for flexural strength. Wollastonite mineral fibers (3–5 wt.%) and styrene–butadiene (SB) latex (5–7 wt.%) were studied to enhance this requirement. Fluidity was evaluated by flow test and measuring the heat of hydration; mechanical properties were evaluated in terms of compressive and flexural strength. The cement-to-silica sand ratio (C:S ratio) was also applied differently to adjust the pot life of polymer cement-based material (1:1 and 1:1.5) as a binder. Because wollastonite mineral fibers and SB latex affect workability, the water-to-binder ratio was regulated to reach the target flow according to the amount of wollastonite mineral fibers and SB latex. Regardless of the C:S ratio, all studied mixtures met the target 28 day compressive strength at 24 h, decreasing in strength with increasing amounts of wollastonite mineral fibers and latex. Flexural strength also fulfilled the target value, and it increased with increasing amounts of wollastonite mineral fibers and latex, unlike compressive strength. The optimal mix proportion of high-early-strength cement-based repair materials constituted 3 wt.% wollastonite mineral fibers and 5 wt.% SB latex as the binder in a C:S ratio of 1:1.5.
A Review on Multiplicity in Multi-Material Additive Manufacturing: Process, Capability, Scale, and Structure
Authors: Verma Ayush , Kapil Angshuman , Klobčar Damjan , Sharma Abhay
Published: July 2023

Additive manufacturing (AM) has experienced exponential growth over the past two decades and now stands on the cusp of a transformative paradigm shift into the realm of multi-functional component manufacturing, known as multi-material AM (MMAM). While progress in MMAM has been more gradual compared to single-material AM, significant strides have been made in exploring the scientific and technological possibilities of this emerging field. Researchers have conducted feasibility studies and investigated various processes for multi-material deposition, encompassing polymeric, metallic, and bio-materials. To facilitate further advancements, this review paper addresses the pressing need for a consolidated document on MMAM that can serve as a comprehensive guide to the state of the art. Previous reviews have tended to focus on specific processes or materials, overlooking the overall picture of MMAM. Thus, this pioneering review endeavors to synthesize the collective knowledge and provide a holistic understanding of the multiplicity of materials and multiscale processes employed in MMAM. The review commences with an analysis of the implications of multiplicity, delving into its advantages, applications, challenges, and issues. Subsequently, it offers a detailed examination of MMAM with respect to processes, materials, capabilities, scales, and structural aspects. Seven standard AM processes and hybrid AM processes are thoroughly scrutinized in the context of their adaptation for MMAM, accompanied by specific examples, merits, and demerits. The scope of the review encompasses material combinations in polymers, composites, metals-ceramics, metal alloys, and biomaterials. Furthermore, it explores MMAM’s capabilities in fabricating bi-metallic structures and functionally/compositionally graded materials, providing insights into various scale and structural aspects. The review culminates by outlining future research directions in MMAM and offering an overall outlook on the vast potential of multiplicity in this field. By presenting a comprehensive and integrated perspective, this paper aims to catalyze further breakthroughs in MMAM, thus propelling the next generation of multi-functional component manufacturing to new heights by capitalizing on the unprecedented possibilities of MMAM.
Biomechanical Analysis of Palateless Splinted and Unsplinted Maxillary Implant-Supported Overdentures: A Three-Dimensional Finite Element Analysis
Authors: Frolo Mária , Řehounek Luboš , Jíra Aleš , Pošta Petr , Hauer Lukáš
Published: July 2023

The objective of this study was to compare the distribution of stress in the maxillary bone, dental implants, and prosthetic components supporting implant-supported maxillary overdentures with partial palatal coverage, in both splinted and unsplinted designs. Two models of maxillary overdentures were designed using the Exocad Dental CAD program, which included cancellous and cortical bone. The complete denture design and abutments (locator abutments in the unsplinted and Hader bar with Vertix attachments placed distally in the splinted variant) were also designed. The denture material was PEEK (Polyetheretherketone), and the method used to analyze patient-specific 3D X-ray scans was 3D QCT/FEA (three-dimensional quantitative computed tomography-based finite element analysis). Loading was divided into three load cases, in the frontal region (both incisors of the denture) and distal region (both molars and first premolar of the denture). The forces applied were 150 N with an oblique component with a buccal inclination of 35° in the frontal region, and 600 N with a buccal inclination of 5° (molars) or solely vertical (premolar) in the distal region. The model with locator abutments showed higher stresses in all load cases in both analyzed implant variants and in the maxilla. The differences in stress distribution between the splinted and unsplinted variants were more significant in the distal region. According to the results of the present study, the amount of stress in bone tissue and dental implant parts was smaller in the splinted, bar-retained variant. The findings of this study can be useful in selecting the appropriate prosthetic design for implant-supported maxillary overdentures with partial palatal coverage.
Effect of Modifier Form on Mechanical Properties of Hypoeutectic Silumin
Authors: Lipiński Tomasz
Published: July 2023

Aluminum–silicon alloys require modification due to their coarse-grained microstructures and resulting low strength properties. So far, research into the modification process has focused on the use of various chemical components and technological processes, the tasks of which are to refine the microstructure and, thus, increase the mechanical properties of the alloy. In this paper, the answer to the question of whether the form of the modifier influences the modification effect of the hypoeutectic silumin will be found. The tests were carried out using the popular silumin AlSi7Mg. To answer our research question, the alloy was modified under comparable conditions using the following elements: Ti, B, and master alloys AlTi1.5 and AlB1.5. Modifiers in the form of Sr and master alloy AlSr1.5 were also used. All mentioned modifiers were produced and introduced into the liquid alloy in the form of a powder and a rod. Master alloys AlSr1.5 were also produced via cooling from the liquid state through cooling in air and the second variant at a speed of 200 °C/s (in the form of powder and a thin strip). The microstructure and mechanical properties were analyzed based on the following measures: tensile strength, elongation, and hardness of silumin. Based on the conducted research, it was found that the form of the modifier also affects the modification effect visible in the form of changes in the microstructure and mechanical properties. For the powder-modified alloy, greater fineness in the eutectic phase (α and B phases) and an increase in all analyzed mechanical properties were obtained.
Moldflow Simulation and Characterization of Pure Copper Fabricated via Metal Injection Molding
Authors: Bahanan Warda , Fatimah Siti , Song Hyunseok , Lee Eun Hye , Kim Dong-Ju , Yang Hae Woong , Woo Chang Hoon , Ryu Jungho , Widiantara I Putu , Ko Young Gun
Published: July 2023

Metal injection molding (MIM) is a representative near-net-shape manufacturing process that fabricates advanced geometrical components for automobile and device industries. As the mechanical performance of an MIM product is affected by green-part characteristics, this work investigated the green part of pure copper processed with MIM using the injection temperature of ~180 °C and injection pressure of ~5 MPa. A computational analysis based on the Moldflow program was proposed to simulate the effectivity of the process by evaluating the confidence of fill, quality prediction, and pressure drop of three distinctive regions in the green part. The results showed that the ring and edge regions of the green parts showed localized behavior, which was related to processing parameters including the position of the gate. A microstructural observation using scanning electron microscopy and a 3D X-ray revealed that both the surface and body matrix consisted of pores with some agglomeration of micro-pores on the edges and ring part, while any critical defects, such as a crack, were not found. A microhardness analysis showed that the three regions exhibited a reasonable uniformity with a slight difference in one specific part mainly due to the localized pore agglomeration. The simulation results showed a good agreement with the microstructures and microhardness data. Thus, the present results are useful for providing guidelines for the sound condition of MIM-treated pure copper with a complex shape.
Using Metal Magnetic Memory to Evaluate the Effect of Welding Method and Weld Temperature on Magnetic Field Strength in Structural Steel
Authors: Wieczorska Agata , Kosoń-Schab Agnieszka
Published: July 2023

Tests of welds are carried out inter-operatively, during the execution of the steel structure, as well as after the structure is welded, but even before its assembly. Steel structures already in service are also examined to detect potential cracks, delamination, or corrosion loss of thickness having the effect of weakening the mechanical strength of the structure. Such examinations are some the elements that comprise a structural health assessment. In this paper, the metal memory method was used to evaluate the effect of the welding method and weld temperature on the change in magnetic field strength. S235JR structural steel was used for the study, which was subjected to milling and MMA, TIG, and MIG welding. The results of measurement experiments carried out for each welding method are presented as graphs.
Briquettes Made of Branches Wood of Three Mangrove Species Bonded by Starch Adhesive
Authors: Tambunan Hardiansyah , Nuryawan Arif , Iswanto Apri Heri , Risnasari Iwan , Basyuni Mohammad , Fatriasari Widya
Published: July 2023

The development and utilization of wood briquettes is one of the efforts to reduce dependence on fossil fuels, including to fulfill overseas market need. This study aimed to evaluate the properties of wood briquettes made of the branches of three mangrove species and to analyze the effect of different wood species of mangrove branches, and the types of starch adhesive, on the quality of wood briquettes. The wood briquettes made in this study were 3 cm × 4 cm in a cylindrical shape using three wood species of mangrove branches, namely mata buaya (Bruguiera sexangula), buta-buta (Excoecaria agallocha), and bakau minyak (Rhizophora apiculata), while the adhesives used were tapioca starch, maize starch, and potato starch. The results showed that the moisture, ash content, and calorific value of the wood briquettes mostly met the ISO 17225-3:2-2020 class A2 standard and the specification and quality standards of wood briquettes for Grade A2 issued by the Korea Forest Research Institute, except the density. Wood briquettes made of mata buaya by using the three types of starch adhesives generally had better properties than all other types of wood briquettes. The interaction of mangrove wood species and the types of starch adhesive had a significant effect on the properties of wood briquettes, except for volatile matter and calorific value for which they had no significant effect. The use of wood briquettes from mangrove wood branches contributes to sustainable forest management and maintains the ecological function of mangrove forests while providing environmentally friendly alternative energy for households as a source of fuel/energy. Furthermore, future research is needed, such as investigating the optimal pressing pressure needed to achieve higher density of the wood briquettes.