bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Algorithm for Capacitated Vehicle Routing Problem for Smart Cities
Authors: Sajid Mohammad , Singh Jagendra , Haidri Raza Abbas , Prasad Mukesh , Varadarajan Vijayakumar , Kotecha Ketan , Garg Deepak
Published: October 2021

Smart logistics is an indispensable building block in smart cities development that requires solving the challenge of efficiently serving the demands of geographically distributed customers by a fleet of vehicles. It consists of a very well-known NP-hard complex optimization problem, which is known as the capacitated vehicle routing problem (CVRP). The CVRP has widespread real-life applications such as delivery in smart logistics, the pharmaceutical distribution of vacancies, disaster relief efforts, and others. In this work, a novel giant tour best cost crossover (GTBCX) operator is proposed which works stochastically to search for the optimal solutions of the CVRP. An NSGA-II-based routing algorithm employing GTBCX is also proposed to solve the CVRP to minimize the total distance traveled as well as to minimize the longest route length. The simulated study is performed on 88 benchmark CVRP instances to validate the success of our proposed GTBCX operator against the nearest neighbor crossover (NNX) and edge assembly crossover (EAX) operators. The rigorous simulation study shows that the GTBCX is a powerful operator and helps to find results that are superior in terms of the overall distance traveled, length of the longest route, quality, and number of Pareto solutions. This work employs a multi-objective optimization algorithm to solve the capacitated vehicle routing problem (CVRP), where the CVRP is represented in the form of a two-dimensional graph. To compute the values’ objective functions, the distance between two nodes in the graph is considered symmetric. This indicates that the genetic algorithm complex optimization algorithm is employed to solve CVRP, which is a symmetry distance-based graph.
Simultaneous Collision of the Rigid Body at Two Points
Authors: Dragna Ionuț-Bogdan , Pandrea Nicolae , Stănescu Nicolae-Doru
Published: October 2021

We present a new approach based on the notion of inertance for the simultaneous collisions without friction of a rigid solid. The calculations are performed using the screw (plückerian) coordinates, while the results are obtained in matrix form, and they may be easily implemented for different practical situations. One calculates the velocities after collision, the energy of lost velocities, and the loss of the kinetic energy. The general algorithm of calculation is described in the paper. The main assumption is that the normal velocities at the contact points vanish simultaneously. The coefficients of restitution at the contact points may be equal or not. Some completely solved applications are also presented, and the numerical results are discussed. The numerical values depend on which coefficient of restitution is used.
Mapping Directional Mid-Air Unistroke Gestures to Interaction Commands: A User Elicitation and Evaluation Study
Authors: Xiao Yiqi , Miao Ke , Jiang Chenhan
Published: October 2021

A stroke is the basic limb movement that both humans and animals naturally and repetitiously perform. Having been introduced into gestural interaction, mid-air stroke gestures saw a wide application range and quite intuitive use. In this paper, we present an approach for building command-to-gesture mapping that exploits the semantic association between interactive commands and the directions of mid-air unistroke gestures. Directional unistroke gestures make use of the symmetry of the semantics of commands, which makes a more systematic gesture set for users’ cognition and reduces the number of gestures users need to learn. However, the learnability of the directional unistroke gestures is varying with different commands. Through a user elicitation study, a gesture set containing eight directional mid-air unistroke gestures was selected by subjective ratings of the direction in respect to its association degree with the corresponding command. We evaluated this gesture set in a following study to investigate the learnability issue, and the directional mid-air unistroke gestures and user-preferred freehand gestures were compared. Our findings can offer preliminary evidence that “return”, “save”, “turn-off” and “mute” are the interaction commands more applicable to using directional mid-air unistrokes, which may have implication for the design of mid-air gestures in human–computer interaction.
Expression, Purification and Refolding of a Human NaV1.7 Voltage Sensing Domain with Native-like Toxin Binding Properties
Authors: Schroder Ryan V. , Cohen Leah S. , Wang Ping , Arizala Joekeem D. , Poget Sébastien F.
Published: October 2021

The voltage-gated sodium channel NaV1.7 is an important target for drug development due to its role in pain perception. Recombinant expression of full-length channels and their use for biophysical characterization of interactions with potential drug candidates is challenging due to the protein size and complexity. To overcome this issue, we developed a protocol for the recombinant expression in E. coli and refolding into lipids of the isolated voltage sensing domain (VSD) of repeat II of NaV1.7, obtaining yields of about 2 mg of refolded VSD from 1 L bacterial cell culture. This VSD is known to be involved in the binding of a number of gating-modifier toxins, including the tarantula toxins ProTx-II and GpTx-I. Binding studies using microscale thermophoresis showed that recombinant refolded VSD binds both of these toxins with dissociation constants in the high nM range, and their relative binding affinities reflect the relative IC50 values of these toxins for full-channel inhibition. Additionally, we expressed mutant VSDs incorporating single amino acid substitutions that had previously been shown to affect the activity of ProTx-II on full channel. We found decreases in GpTx-I binding affinity for these mutants, consistent with a similar binding mechanism for GpTx-I as compared to that of ProTx-II. Therefore, this recombinant VSD captures many of the native interactions between NaV1.7 and tarantula gating-modifier toxins and represents a valuable tool for elucidating details of toxin binding and specificity that could help in the design of non-addictive pain medication acting through NaV1.7 inhibition.
Pyrrolizidine Alkaloid-Induced Hepatotoxicity Associated with the Formation of Reactive Metabolite-Derived Pyrrole–Protein Adducts
Authors: Ma Jiang , Li Mi , Li Na , Chan Wood Yee , Lin Ge
Published: October 2021

Pyrrolizidine alkaloids (PAs) with 1,2-unsaturated necine base are hepatotoxic phytotoxins. Acute PA intoxication is initiated by the formation of adducts between PA-derived reactive pyrrolic metabolites with cellular proteins. The present study aimed to investigate the correlation between the formation of hepatic pyrrole–protein adducts and occurrence of PA-induced liver injury (PA-ILI), and to further explore the use of such adducts for rapidly screening the hepatotoxic potency of natural products which contain PAs. Aqueous extracts of Crotalaria sessiliflora (containing one PA: monocrotaline) and Gynura japonica (containing two PAs: senecionine and seneciphylline) were orally administered to rats at different doses for 24 h to investigate PA-ILI. Serum alanine aminotransferase (ALT) activity, hepatic glutathione (GSH) level, and liver histological changes of the treated rats were evaluated to assess the severity of PA-ILI. The levels of pyrrole–protein adducts formed in the rats’ livers were determined by a well-established spectrophotometric method. The biological and histological results showed a dose-dependent hepatotoxicity with significantly different toxic severity among groups of rats treated with herbal extracts containing different PAs. Both serum ALT activity and the amount of hepatic pyrrole–protein adducts increased in a dose-dependent manner. Moreover, the elevation of ALT activity correlated well with the formation of hepatic pyrrole–protein adducts, regardless of the structures of different PAs. The findings revealed that the formation of hepatic pyrrole–protein adducts—which directly correlated with the elevation of serum ALT activity—was a common insult leading to PA-ILI, suggesting a potential for using pyrrole–protein adducts to screen hepatotoxicity and rank PA-containing natural products, which generally contain multiple PAs with different structures.
Pregnancy, Birth, Infant, and Early Childhood Neurodevelopmental Outcomes among a Cohort of Women with Symptoms of Zika Virus Disease during Pregnancy in Three Surveillance Sites, Project Vigilancia de Embarazadas con Zika (VEZ), Colombia, 2016–2018
Authors: Mercado-Reyes Marcela , Gilboa Suzanne M. , Valencia Diana , Daza Marcela , Tong Van T. , Galang Romeo R. , Winfield Christina M. , Godfred-Cato Shana , Benavides Mónica , Villanueva Julie M. , Thomas Jennifer D. , Daniels Jonathan , Zaki Sherif , Reagan-Steiner Sarah , Bhatnagar Julu , Schiffer Jarad , Steward-Clark Evelene , Ricaldi Jessica N. , Osorio Johana , Sancken Christina L. , Pardo Lissethe , Tinker Sarah C. , Anderson Kayla N. , Rico Angelica , Burkel Veronica K. , Hojnacki Jacob , Delahoy Miranda J. , González Maritza , Osorio May B. , Moore Cynthia A. , Honein Margaret A. , Ospina Martinez Martha Lucia
Published: October 2021

Project Vigilancia de Embarazadas con Zika (VEZ), an intensified surveillance of pregnant women with symptoms of the Zika virus disease (ZVD) in Colombia, aimed to evaluate the relationship between symptoms of ZVD during pregnancy and adverse pregnancy, birth, and infant outcomes and early childhood neurodevelopmental outcomes. During May–November 2016, pregnant women in three Colombian cities who were reported with symptoms of ZVD to the national surveillance system, or with symptoms of ZVD visiting participating clinics, were enrolled in Project VEZ. Data from maternal and pediatric (up to two years of age) medical records were abstracted. Available maternal specimens were tested for the presence of the Zika virus ribonucleic acid and/or anti-Zika virus immunoglobulin antibodies. Of 1213 enrolled pregnant women with symptoms of ZVD, 1180 had a known pregnancy outcome. Results of the Zika virus laboratory testing were available for 569 (48.2%) pregnancies with a known pregnancy outcome though testing timing varied and was often distal to the timing of symptoms; 254 (21.5% of the whole cohort; 44.6% of those with testing results) were confirmed or presumptive positive for the Zika virus infection. Of pregnancies with a known outcome, 50 (4.2%) fetuses/infants had Zika-associated brain or eye defects, which included microcephaly at birth. Early childhood adverse neurodevelopmental outcomes were more common among those with Zika-associated birth defects than among those without and more common among those with laboratory evidence of a Zika virus infection compared with the full cohort. The proportion of fetuses/infants with any Zika-associated brain or eye defect was consistent with the proportion seen in other studies. Enhancements to Colombia’s existing national surveillance enabled the assessment of adverse outcomes associated with ZVD in pregnancy.
Phage Therapy Related Microbial Succession Associated with Successful Clinical Outcome for a Recurrent Urinary Tract Infection
Authors: Terwilliger Austen , Clark Justin , Karris Maile , Hernandez-Santos Haroldo , Green Sabrina , Aslam Saima , Maresso Anthony
Published: October 2021

We rationally designed a bacteriophage cocktail to treat a 56-year-old male liver transplant patient with complex, recurrent prostate and urinary tract infections caused by an extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) (UCS1). We screened our library for phages that killed UCS1, with four promising candidates chosen for their virulence, mucolytic properties, and ability to reduce bacterial resistance. The patient received 2 weeks of intravenous phage cocktail with concomitant ertapenem for 6 weeks. Weekly serum and urine samples were collected to track the patient’s response. The patient tolerated the phage therapy without any adverse events with symptom resolution. The neutralization of the phage activity occurred with sera collected 1 to 4 weeks after the first phage treatment. This was consistent with immunoassays that detected the upregulation of immune stimulatory analytes. The patient developed asymptomatic recurrent bacteriuria 6 and 11 weeks following the end of phage therapy—a condition that did not require antibiotic treatment. The bacteriuria was caused by a sister strain of E. coli (UCS1.1) that remained susceptible to the original phage cocktail and possessed putative mutations in the proteins involved in adhesion and invasion compared to UCS1. This study highlights the utility of rationally designed phage cocktails with antibiotics at controlling E. coli infection and suggests that microbial succession, without complete eradication, may produce desirable clinical outcomes.
Rapid and Successful Implementation of a COVID-19 Convalescent Plasma Programme—The South African Experience
Authors: Glatt Tanya Nadia , Hilton Caroline , Nyoni Cynthia , Swarts Avril , Swanevelder Ronel , Cowley James , Mmenu Cordelia , Moyo-Gwete Thandeka , Moore Penny L. , Kutama Munzhedzi , Jaza Jabulisile , Phayane Itumeleng , Brits Tinus , Koekemoer Johan , Jentsch Ute , Nelson Derrick , van den Berg Karin , Vermeulen Marion
Published: October 2021

Background: COVID-19 convalescent plasma (CCP) has been considered internationally as a treatment option for COVID-19. CCP refers to plasma collected from donors who have recovered from and made antibodies to SARS-CoV-2. To date, convalescent plasma has not been collected in South Africa. As other investigational therapies and vaccination were not widely accessible, there was an urgent need to implement a CCP manufacture programme to service South Africans. Methods: The South African National Blood Service and the Western Cape Blood Service implemented a CCP programme that included CCP collection, processing, testing and storage. CCP units were tested for SARS-CoV-2 Spike ELISA and neutralising antibodies and routine blood transfusion parameters. CCP units from previously pregnant females were tested for anti-HLA and anti-HNA antibodies. Results: A total of 987 CCP units were collected from 243 donors, with a median of three donations per donor. Half of the CCP units had neutralising antibody titres of >1:160. One CCP unit was positive on the TPHA serology. All CCP units tested for anti-HLA antibodies were positive. Conclusion: Within three months of the first COVID-19 diagnosis in South Africa, a fully operational CCP programme was set up across South Africa. The infrastructure and skills implemented will likely benefit South Africans in this and future pandemics.
Metabolic Reprogramming of Nasal Airway Epithelial Cells Following Infant Respiratory Syncytial Virus Infection
Authors: Connelly Andrew R. , Jeong Brian M. , Coden Mackenzie E. , Cao Jacob Y. , Chirkova Tatiana , Rosas-Salazar Christian , Cephus Jacqueline-Yvonne , Anderson Larry J. , Newcomb Dawn C. , Hartert Tina V. , Berdnikovs Sergejs
Published: October 2021

Respiratory syncytial virus (RSV) is a seasonal mucosal pathogen that infects the ciliated respiratory epithelium and results in the most severe morbidity in the first six months of life. RSV is a common cause of acute respiratory infection during infancy and is an important early-life risk factor strongly associated with asthma development. While this association has been repeatedly demonstrated, limited progress has been made on the mechanistic understanding in humans of the contribution of infant RSV infection to airway epithelial dysfunction. An active infection of epithelial cells with RSV in vitro results in heightened central metabolism and overall hypermetabolic state; however, little is known about whether natural infection with RSV in vivo results in lasting metabolic reprogramming of the airway epithelium in infancy. To address this gap, we performed functional metabolomics, 13C glucose metabolic flux analysis, and RNA-seq gene expression analysis of nasal airway epithelial cells (NAECs) sampled from infants between 2–3 years of age, with RSV infection or not during the first year of life. We found that RSV infection in infancy was associated with lasting epithelial metabolic reprogramming, which was characterized by (1) significant increase in glucose uptake and differential utilization of glucose by epithelium; (2) altered preferences for metabolism of several carbon and energy sources; and (3) significant sexual dimorphism in metabolic parameters, with RSV-induced metabolic changes most pronounced in male epithelium. In summary, our study supports the proposed phenomenon of metabolic reprogramming of epithelial cells associated with RSV infection in infancy and opens exciting new venues for pursuing mechanisms of RSV-induced epithelial barrier dysfunction in early life.
Genetic Characterization and Pathogenicity of H7N7 and H7N9 Avian Influenza Viruses Isolated from South Korea
Authors: Na Eun-Jee , Kim Young-Sik , Kim Yoon-Ji , Park Jun-Soo , Oem Jae-Ku
Published: October 2021

H7 low pathogenic avian influenza viruses (LPAIVs) can mutate into highly pathogenic avian influenza viruses (HPAIVs). In addition to avian species, H7 avian influenza viruses (AIVs) also infect humans. In this study, two AIVs, H7N9 (20X-20) and H7N7 (34X-2), isolated from the feces of wild birds in South Korea in 2021, were genetically analyzed. The HA cleavage site of the two H7 Korean viruses was confirmed to be ELPKGR/GLF, indicating they are LPAIVs. There were no amino acid substitutions at the receptor-binding site of the HA gene of two H7 Korean viruses compared to that of A/Anhui/1/2013 (H7N9), which prefer human receptors. In the phylogenetic tree analysis, the HA gene of the two H7 Korean viruses shared the highest nucleotide similarity with the Korean H7 subtype AIVs. In addition, the HA gene of the two H7 Korean viruses showed high nucleotide similarity to that of the A/Jiangsu/1/2018(H7N4) virus, which is a human influenza virus originating from avian influenza virus. Most internal genes (PB2, PB1, PA, NP, NA, M, and NS) of the two H7 Korean viruses belonged to the Eurasian lineage, except for the M gene of 34X-2. This result suggests that active reassortment occurred among AIVs. In pathogenicity studies of mice, the two H7 Korean viruses replicated in the lungs of mice. In addition, the body weight of mice infected with 34X-2 decreased 7 days post-infection (dpi) and inflammation was observed in the peribronchiolar and perivascular regions of the lungs of mice. These results suggest that mammals can be infected with the two H7 Korean AIVs. Our data showed that even low pathogenic H7 AIVs may infect mammals, including humans, as confirmed by the A/Jiangsu/1/2018(H7N4) virus. Therefore, continuous monitoring and pathogenicity assessment of AIVs, even of LPAIVs, are required.
Identification of Human Norovirus GII.3 Blockade Antibody Epitopes
Authors: Yi Yufang , Wang Shuxia , Wang Xiaoli , Xiong Pei , Liu Qingwei , Zhang Chao , Yin Feifei , Huang Zhong
Published: October 2021

Human noroviruses are a common pathogen causing acute gastroenteritis worldwide. Among all norovirus genotypes, GII.3 is particularly prevalent in the pediatric population. Here we report the identification of two distinct blockade antibody epitopes on the GII.3 capsid. We generated a panel of monoclonal antibodies (mAbs) from mice immunized with virus-like particle (VLP) of a GII.3 cluster 3 strain. Two of these mAbs, namely 8C7 and 8D1, specifically bound the parental GII.3 VLP but not VLPs of GII.4, GII.17, or GI.1. In addition, 8C7 and 8D1 efficiently blocked GII.3 VLP binding with its ligand, histo-blood group antigens (HBGA). These data demonstrate that 8C7 and 8D1 are GII.3-specific blockade antibodies. By using a series of chimeric VLPs, we mapped the epitopes of 8C7 and 8D1 to residues 385–400 and 401–420 of the VP1 capsid protein, respectively. These two blockade antibody epitopes are highly conserved among GII.3 cluster 3 strains. Structural modeling shows that the 8C7 epitope partially overlaps with the HBGA binding site (HBS) while the 8D1 epitope is spatially adjacent to HBS. These findings may enhance our understanding of the immunology and evolution of GII.3 noroviruses.
BNT162b2 mRNA Vaccination Leads to Long-Term Protection from COVID-19 Disease
Authors: Rossi Claudia , Lanuti Paola , Cicalini Ilaria , De Bellis Domenico , Pierdomenico Laura , Del Boccio Piero , Zucchelli Mirco , Natale Luca , Sinjari Bruna , Catitti Giulia , Vespa Simone , Simeone Pasquale , Bologna Giuseppina , Bucci Ines , Falasca Katia , Vecchiet Jacopo , Stuppia Liborio , De Laurenzi Vincenzo , Pieragostino Damiana
Published: October 2021

The efficacy of SARS-CoV-2 mRNA-based vaccines in preventing COVID-19 disease has been extensively demonstrated; however, it is of uttermost importance to acquire knowledge on the persistence of immune-protection both in terms of levels of neutralizing antibodies and specialized memory cells. This can provide important scientific basis for decisions on the need of additional vaccine doses and on when these should be administered thus resulting in an improvement in vaccination schedules. Here, we briefly report the changes in antibody levels and cellular immunity following BNT162b2 administration. We show an important fall in anti S1-Spike antibodies in BNT162b2 vaccinated subjects overtime, paralleled by a contextual consolidation of specific spike (S) T-cells, mainly of the CD8+ compartment. Contrariwise, CD4+ S-specific response shows a considerable interindividual variability. These data suggest that the well-known antibody drop in vaccinated subjects is replaced by memory cell consolidation that can protect from severe adverse effects of SARS-CoV-2 infection.
Public Willingness and Determinants of COVID-19 Vaccination at the Initial Stage of Mass Vaccination in China
Authors: Zhao Yi-Miao , Liu Lin , Sun Jie , Yan Wei , Yuan Kai , Zheng Yong-Bo , Lu Zheng-An , Liu Lin , Ni Shu-Yu , Su Si-Zhen , Zhu Xi-Mei , Zeng Na , Gong Yi-Miao , Wu Ping , Ran Mao-Sheng , Leng Yue , Shi Jie , Shi Le , Lu Lin , Bao Yan-Ping
Published: October 2021

The present study assessed the willingness of the general population to receive COVID-19 vaccines and identified factors that influence vaccine hesitancy and resistance. A national online survey was conducted from 29 January 2021 to 26 April 2021 in China. Multinomial logistic regression analyses were conducted to identify factors that influence vaccine hesitancy and resistance. Of the 34,041 participants surveyed, 18,810 (55.3%) were willing to get vaccinated, 13,736 (40.3%) were hesitant, and 1495 (4.4%) were resistant. Rates of vaccine acceptance increased over time, with geographical discrepancies in vaccine hesitancy and resistance between provinces in China. Vaccine safety was the greatest concern expressed by most participants (24,461 [71.9%]), and the major reason for participants’ refusing vaccination (974 [65.2%]). Government agencies (23,131 [68.0%]) and social media (20,967 [61.6%]) were the main sources of COVID-19 vaccine information. Compared with vaccination acceptance, female, young and middle-aged, high income, and perceived low-risk of infection were associated with vaccine hesitancy. Histories of allergic reactions to other vaccines and depression symptoms were related to vaccine resistance. Common factors that influenced vaccine hesitancy and resistance were residing in cities and perceiving less protection with vaccines than with other protective measures. The results indicate that the rate of vaccine resistance is relatively low, but vaccine hesitancy is common. Individuals who are female, young and middle-aged, with a high income, and residing in cities are more likely to be hesitant for vaccination and should be the target populations for vaccination campaigns. Specific vaccine messaging from the government and social media could alleviate public concerns about vaccine safety and efficacy.
From Microcars to Heavy-Duty Vehicles: Vehicle Performance Comparison of Battery and Fuel Cell Electric Vehicles
Authors: Sagaria Shemin , Moreira António , Margarido Fernanda , Baptista Patricia
Published: October 2021

Low vehicle occupancy rates combined with record conventional vehicle sales justify the requirement to optimize vehicle type based on passengers and a powertrain with zero-emissions. This study compares the performance of different vehicle types based on the number of passengers/payloads, powertrain configuration (battery and fuel cell electric configurations), and drive cycles, to assess range and energy consumption. An adequate choice of vehicle segment according to the real passenger occupancy enables the least energy consumption. Vehicle performance in terms of range points to remarkable results for the FCEV (fuel cell electric vehicle) compared to BEV (battery electric vehicle), where the former reached an average range of 600 km or more in all different drive cycles, while the latter was only cruising nearly 350 km. Decisively, the cost analysis indicated that FCEV remains the most expensive option with base cost three-fold that of BEV. The FCEV showed notable results with an average operating cost of less than 7 cents/km, where BEV cost more than 10 €/km in addition to the base cost for light-duty vehicles. The cost analysis for a bus and semi-truck showed that with a full payload, FCPT (fuel cell powertrain) would be more economical with an average energy cost of ~1.2 €/km, while with BPT the energy cost is more than 300 €/km.
Ischiocavernosus Muscle Release for Urethral Obstruction Treatment after Pelvic Symphyseal Distraction Osteotomy in a Cat with Pelvic Stenosis
Authors: Roh Yoon-Ho , Kim Jeong-Nam , Byun Pill-Moo , Kim Dae-Hyun , Jeong Seong-Mok , Lee Hae-Beom
Published: October 2021

Symphyseal distraction osteotomy (SDO) with a polymethyl methacrylate (PMMA) spacer is an effective surgical treatment for cats with pelvic stenosis. This study reports the successful treatment of urethral obstruction due to ischiocavernosus muscle (IM) tension after SDO with a PMMA spacer. A 2-year-old castrated male Korean domestic shorthair feline had megacolon and pelvic canal stenosis. The ratio of the maximal diameter of the colon to the L5 length and the pelvic canal diameter ratio were 1.6 and 0.45, respectively. Pelvic SDO was performed with a PMMA spacer, leading to pelvic canal enlargement (pelvic canal diameter ratio: 0.73). Two days after surgery, dysuria was identified immediately after removing the preoperatively placed urinary catheter. Complete blood counts and serum biochemical profiles were within the reference intervals, and a positive contrast retrograde urethrogram confirmed urethral obstruction at the level of the membranous–pelvic urethra region. Increased tension of the IM leading to a narrowed urethra was suspected as the cause of urethral obstruction. After IM release at the level of origin on the ischium, the patient had an uneventful recovery with spontaneous urination. Muscle release resulted in excellent functional restoration, with no intraoperative or postoperative complications reported during the 12-month long-term follow-up. Therefore, SDO with IM release could be a feasible therapeutic option for severe pelvic stenosis without complications, such as urethral obstruction, in cats.
A Review of Ethnoveterinary Knowledge, Biological Activities and Secondary Metabolites of Medicinal Woody Plants Used for Managing Animal Health in South Africa
Authors: Selogatwe Kelebogile Martha , Asong John Awungnjia , Struwig Madeleen , Ndou Rendani Victress , Aremu Adeyemi Oladapo
Published: October 2021

Globally, the use of ethnoveterinary medicine as remedies for animal health among different ethnic groups justify the need for a systematic exploration to enhance their potential. In addition, the increasing popularity and utilisation of woody plants remain common in traditional medicine, which may be attributed to their inherent benefits. The current review was aimed at analysing ethnoveterinary surveys, biological activities, and secondary metabolites/phytochemical profiles of the woody plants of South Africa. Eligible literature (period: 2000 to 2020) were retrieved from different databases such as Google Scholar, PubMed, Sabinet, and Science Direct. Based on the inclusion and exclusion criteria, 20 ethnoveterinary surveys were eligible and were subjected to further analysis. We identified 104 woody plant species from 44 plant families that are used in the treatment of different diseases in animals, particularly cattle (70%) and goats (20%). The most mentioned (with six citations) woody plants were Terminalia sericea Burch. ex DC and Ziziphus mucronata Willd., which were followed by plants with five (Cussonia spicata Thunb., Pterocarpus angolensis DC and Vachellia karroo (Hayne) Banfi & Galasso) or four (Acokanthera oppositifolia (Lam.) Codd, Cassia abbreviata Oliv., and Strychnos henningsii Gilg) individual mentions. The most dominant families were Fabaceae (19%), Apocynaceae (5.8%), Rubiaceae (5.8%), Anacardiaceae (4.8%), Combretaceae (4.8%), Euphorbiaceae (4.8%), Malvaceae (4.8%), Rhamnaceae (4.8%), and Celastraceae (3.8%). Bark (33%), leaves (29%), and roots (19%) were the plant parts dominantly used to prepare remedies for ethnoveterinary medicine. An estimated 20% of woody plants have been screened for antimicrobial, anthelmintic, antioxidant, and cytotoxicity effects. Phytochemical profiles established a rich pool of valuable secondary metabolites (phenolic, flavonoids and condensed tannins) that may be responsible for the exerted biological activities. Overall, the significant portion of woody plants lacking empirical evidence on their biological effects indicates a major knowledge gap that requires more research efforts.
Therapeutic Effects of Risperidone against Spinal Cord Injury in a Rat Model of Asphyxial Cardiac Arrest: A Focus on Body Temperature, Paraplegia, Motor Neuron Damage, and Neuroinflammation
Authors: Lee Tae-Kyeong , Lee Jae-Chul , Tae Hyun-Jin , Kim Hyung-Il , Shin Myoung Cheol , Ahn Ji Hyeon , Park Joon Ha , Kim Dae Won , Hong Seongkweon , Choi Soo Young , Cho Jun Hwi , Won Moo-Ho
Published: October 2021

Cardiac arrest (CA) causes severe spinal cord injury and evokes spinal cord disorders including paraplegia. It has been reported that risperidone, an antipsychotic drug, effectively protects neuronal cell death from transient ischemia injury in gerbil brains. However, until now, studies on the effects of risperidone on spinal cord injury after asphyxial CA (ACA) and cardiopulmonary resuscitation (CPR) are not sufficient. Therefore, this study investigated the effect of risperidone on hind limb motor deficits and neuronal damage/death in the lumbar part of the spinal cord following ACA in rats. Mortality, severe motor deficits in the hind limbs, and the damage/death (loss) of motor neurons located in the anterior horn were observed two days after ACA/CPR. These symptoms were significantly alleviated by risperidone (an atypical antipsychotic) treatment after ACA. In vehicle-treated rats, the immunoreactivities of tumor necrosis factor-alpha (TNF-α) and interleukin 1-beta (IL-1β), as pro-inflammatory cytokines, were increased, and the immunoreactivities of IL-4 and IL-13, as anti-inflammatory cytokines, were reduced with time after ACA/CPR. In contrast, in risperidone-treated rats, the immunoreactivity of the pro-inflammatory cytokines was significantly decreased, and the anti-inflammatory cytokines were enhanced compared to vehicle-treated rats. In brief, risperidone treatment after ACA/CPR in rats significantly improved the survival rate and attenuated paralysis, the damage/death (loss) of motor neurons, and inflammation in the lumbar anterior horn. Thus, risperidone might be a therapeutic agent for paraplegia by attenuation of the damage/death (loss) of spinal motor neurons and neuroinflammation after ACA/CPR.
Water Demand Management Based on Water Consumption Data Analysis in the Emirate of Abu Dhabi
Authors: Albannay Shayma , Kazama Shinobu , Oguma Kumiko , Hashimoto Takashi , Takizawa Satoshi
Published: October 2021

In Abu Dhabi, in the United Arab Emirates (UAE), the population and water demand have nearly tripled in the last two decades. Thus, it has become critical to curtail the growing water demand. This study aimed to evaluate the efficacy of Abu Dhabi’s residential water demand management through the installation of water-saving fittings. The analysis of water consumption time-series data revealed that water consumption of the three water user categories was significantly different. Briefly: water-tariff exempt users consumed 95.19 m3/d/connection in 2019, followed by nationals with 5.14 m3/d/connection, and expats at 0.7 m3/d/connection. Nationals began saving water in 2011, which was earlier than the water tariff revision of 2015. In a newly developed area of Khalifa City, the water consumption of new residents was 46.0% less than that of old residents, indicating the effectiveness of water-saving fittings installed in new homes following the Housing Rules’ requirements. Then, based on the estimated number of new connections and the fittings’ saving efficiencies, we estimated that water-saving fittings contributed to 73.1% of the water savings since 2011. These results strongly recommend the introduction of an incentive or subsidy for owners of old houses to replace their outdated fittings with water-saving ones.
Assessment of the Disaster Resilience of Complex Systems: The Case of the Flood Resilience of a Densely Populated City
Authors: Arosio Marcello , Cesarini Luigi , Martina Mario L. V.
Published: October 2021

In the last decades, resilience became officially the worldwide cornerstone to reduce the risk of disasters and improve preparedness, response, and recovery capacities. Although the concept of resilience is now clear, it is still under debate how to model and quantify it. The aim of this work was to quantify the resilience of a complex system, such as a densely populated and urbanized area, by modelling it with a graph, the mathematical representation of the system element and connections. We showed that the graph can account for the resilience characteristics included in its definition according to the United Nations General Assembly, considering two significant aspects of this definition in particular: (1) resilience is a property of a system and not of single entities and (2) resilience is a property of the system dynamic response. We proposed to represent the exposed elements of the system and their connections (i.e., the services they exchange) with a weighted and redundant graph. By mean of it, we assessed the systemic properties, such as authority and hub values and highlighted the centrality of some elements. Furthermore, we showed that after an external perturbation, such as a hazardous event, each element can dynamically adapt, and a new graph configuration is set up, taking advantage of the redundancy of the connections and the capacity of each element to supply lost services. Finally, we proposed a quantitative metric for resilience as the actual reduction of the impacts of events at different return periods when resilient properties of the system are activated. To illustrate step by step the proposed methodology and show its practical feasibility, we applied it to a pilot study: the city of Monza, a densely populated urban environment exposed to river and pluvial floods.
Large-Volume Samplers for Efficient Composite Sampling and Particle Characterization in Sewer Systems
Authors: Nickel Jan Philip , Fuchs Stephan
Published: October 2021

The assessment of pollution from sewer discharges requires flexible and reliable sampling methods. The characteristics of the sampling system must be known to allow comparison with other studies. Large volume samplers (LVS) are increasingly used for monitoring in sewer systems and surface waters. This article provides a comprehensive description of this widely applicable sampling system, gives insight into its comparability to standard methods, and provides recommendations for researchers and practitioners involved in water quality monitoring and urban water management. Two methods for subsampling from LVS are presented, i.e., collection of homogenized or sedimented samples. Results from a sampling campaign at combined sewer overflows (CSOs) were used to investigate the comparability of both subsampling methods and conventional autosamplers (AS). Event mean concentrations (EMC) of total suspended solids (TSS) derived from homogenized LVS samples and AS pollutographs were comparable. TSS-EMC of homogenized and sedimented LVS samples were also comparable. However, differences were found for particle size distribution and organic matter content. Consequently, sedimented LVS samples, which contained solids masses in the range of 3–70 g, are recommended to be used for particle characterization. The differences between homogenized and sedimented LVS samples, e.g., the quality of homogenization and the stability of samples during sedimentation in LVS, should be further investigated. Based on LVS results, average TSS concentrations of 50–60 mg/L were found for CSOs from centralized treatment facilities in Bavaria. With a median share of 84%, particles <63 µm were the dominant fraction.
Are Rural and Small Community Aerated Wastewater Stabilization Ponds a Neglected Source of Microplastic Pollution?
Authors: Gao Zhiqiang , Cizdziel James V. , Wontor Kendall , Lu Haitao
Published: October 2021

Wastewater treatment systems collect and treat sewage that includes microplastics (MPs). However, we are not aware of any studies on the occurrence and distribution of MPs in wastewater stabilization ponds (WSPs), which serve small communities worldwide. Here, we characterized MPs (~45 µm–5 mm) in an aerated WSP serving ~500 houses and an adjacent lake. Putative MPs were most abundant in duckweed (Lemna minor) and sludge (75 ± 22 and 12.8 ± 3.1 particles/g, respectively: ±1 standard deviation (SD), n = 6, dry weight). In the water, average concentrations (particles/L ± 1 SD, n = 6) were highest in the pond (4.1 ± 0.6), followed by effluent (3.9 ± 0.5) and the lake (2.6 ± 0.6). Over 20 types of MPs were identified in each different compartment, with the distribution varying somewhat between the water, sludge, and duckweed. Polyester and polyethylene were the predominant types, followed by polyethylene terephthalate, polyacrylate, polyvinyl chloride, polystyrene, and others. Morphologies consisted of fibers (62–71%), fragments (28–37%), and beads (1–6%). High-density polymers were more frequently found in sludge. Potential sources of the MPs include synthetic textiles from laundry and other plastics washed down household drains. Overall, with ~786,000 MPs/day released in the pond effluent and with duckweed a source of food for waterfowl, we demonstrate that WSPs can be point sources of MPs to both aquatic and terrestrial ecosystems and thus deserve further scrutiny.
Analyses of Protection and Conservation According to the Fish Act 1950 in Bangladesh’s Kaptai Lake Fisheries Management
Authors: Rayhan Nafis , Schneider Petra , Islam Md. Shahidul , Rashid Aminur , Mozumder Mohammad Mojibul Hoque , Hossain Mohammad Mosarof , Begum Amany , Shamsuzzaman Md. Mostafa
Published: October 2021

Kaptai Lake (KL), the largest inland watershed in Bangladesh (ca. 700 km2) and one of Southeast Asia’s largest artificial reservoirs, features an abundant variety of indigenous fishery species. Moreover, it provides a plethora of ecological benefits to society. Nevertheless, the KL is suffering from multidimensional natural and anthropogenic stressors that threaten these wetlands’ sustainability. Though the legal framework assures sustainable conservation of fisheries resources, the implementation scenarios of fisheries laws, regulations, and policies in the KL wetland are insufficient. This study aimed at assessing the fisher’s perception of the regulation implementation efficiency of the Protection and Conservation Fish Act of 1950, while analyzing the effectiveness of the legal framework in the context of biodiversity conservation and the management sustainability of KL. By integrating qualitative and quantitative data collected through participatory Rural Appraisal (PRA) tools viz. 225 interviews with fishers, four focus group discussions, and 12 key informant interviews, the investigation was performed in four selected areas in KL. The findings show that fishers routinely disregard laws and restrictions of the Protection and Conservation of Fish Act 1950 due to various socioeconomic and political forces. Although the annual fish harvest rate from KL appears to be increasing, the lake is losing its charismatic biological diversity primarily due to inappropriate and ineffective enforcement of fishing regulations. Many fishers believe that they still follow the act’s significant laws and regulations while being involved in several destructive and prohibited fishing practices. There is a link between community awareness, the scope of the act’s provisions, the effectiveness of its enforcement, and the strength of its execution. One of the leading causes of biodiversity loss in the KL is inadequate and ineffective fishing regulations. Improvement in the enforcement of the fishing act may be the prominent option to ensure better biodiversity conservation and sustainable management of this wetland. This result calls for functional and policy attention to revising the regulations to account for socioeconomic and political elements contributing to environmental degradation. This study also highlights the urgent need for transdisciplinary collaboration initiatives and synchronous cooperation among the agencies in order to effectively implement the fishing laws and contribute to better conservation and sustainability of the Kaptai lake fisheries resources.
Digested Sludge Quality in Mesophilic, Thermophilic and Temperature-Phased Anaerobic Digestion Systems
Authors: Lanko Iryna , Hejnic Jakub , Říhová-Ambrožová Jana , Ferrer Ivet , Jenicek Pavel
Published: October 2021

Anaerobic digestion (AD) technology is commonly used to treat sewage sludge from activated sludge systems, meanwhile alleviating the energy demand (and costs) for wastewater treatment. Most often, anaerobic digestion is run in single-stage systems under mesophilic conditions, as this temperature regime is considered to be more stable than the thermophilic one. However, it is known that thermophilic conditions are advantageous over mesophilic ones in terms of methane production and digestate hygienisation, while it is unclear which one is better concerning the digestate dewaterability. Temperature-phased anaerobic digestion (TPAD) is a double-stage AD process that combines the above-mentioned temperature regimes, by operating a thermophilic digester followed by a mesophilic one. The aim of this study is to compare the digestate quality of single-stage mesophilic and thermophilic AD and TPAD systems, in terms of the dewaterability, pathogenic safety and lower calorific value (LCV) and, based on the comparison, consider digested sludge final disposal alternatives. The research is conducted in lab-scale reactors treating waste-activated sludge. The dewaterability is tested by two methods, namely, centrifugation and mechanical pressing. The experimental results show that the TPAD system is the most beneficial in terms of organic matter degradation efficiency (32.4% against 27.2 for TAD and 26.0 for MAD), producing a digestate with a high dewaterability (8.1–9.8% worse than for TAD and 6.2–12.0% better than for MAD) and pathogenic safety (coliforms and Escherichia coli were not detected, and Clostridium perfringens were counted up to 4.8–4.9 × 103, when for TAD it was only 1.4–2.5 × 103, and for MAD it was 1.3–1.8 × 104), with the lowest LCV (19.2% against 15.4% and 15.8% under thermophilic and mesophilic conditions, respectively). Regarding the final disposal, the digested sludge after TAD can be applied directly in agriculture; after TPAD, it can be used as a fertilizer only in the case where the fermenter HRT assures the pathogenic safety. The MAD digestate is the best for being used as a fuel preserving a higher portion of organic matter, not transforming into biogas during AD.
Temporal Changes in Flow Regime along the River Vistula
Authors: Bogdanowicz Ewa , Karamuz Emilia , Romanowicz Renata Julita
Published: October 2021

The flow regime in the River Vistula is influenced by climatic and geographical factors and human intervention. In this study, we focus on an analysis of flow and precipitation variability over time and space following the course of the River Vistula. Multi-purpose statistical analyses of a number of runoff and precipitation characteristics were performed to present a general overview of the temporal and spatial changes. Since the important feature of the hydrological regime of Polish rivers is the seasonality of runoff associated with the occurrence of cold (winter) and warm (summer) seasons within a hydrological year, a seasonal approach is applied to describe specific seasonal features that can be masked when using annual data. In general, the results confirm popular impressions about changes in winter season runoff characteristics, i.e., significantly decreasing daily maxima, increasing daily minima and a decrease in concentration, and so a bigger uniformity of winter daily flows. An interesting behaviour of minimum flows in the summer has been revealed, which is contrary to social perceptions and the alarming changes taking place in the other parts of the world. Additionally, precipitation indexes related to the formation of droughts show no trends, e.g., the mean value of the maximum dry spell length.
Smoothed Particle Hydrodynamics Simulation of a Mariculture Platform under Waves
Authors: Zhang Feng , Zhang Li , Xie Yanshuang , Wang Zhiyuan , Shang Shaoping
Published: October 2021

This work investigates the dynamic behaviors of floating structures with moorings using open-source software for smoothed particle hydrodynamics. DualSPHysics permits us to use graphics processing units to recreate designs that include complex calculations at high resolution with reasonable computational time. A free damped oscillation was simulated, and its results were compared with theoretical data to validate the numerical model developed. The simulated three degrees of freedom (3-DoF) (surge, heave, and pitch) of a rectangular floating box have excellent consistency with experimental data. MoorDyn was coupled with DualSPHysics to include a mooring simulation. Finally, we modelled and simulated a real mariculture platform on the coast of China. We simulated the 3-DoF of this mariculture platform under a typical annual wave and a Typhoon Dujuan wave. The motion was light and gentle under the typical annual wave but vigorous under the Typhoon Dujuan wave. Experiments at different tidal water levels revealed an earlier motion response and smaller motion range during the high tide. The results reveal that DualSPHysics combined with MoorDyn is an adaptive scheme to simulate a coupled fluid–solid–mooring system. This work provides support to disaster warning, emergency evacuation, and proper engineering design.