bluuebunny/crossref_metadata_2025 huggingface dataset:
Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
Authors: Sega Pawel , Kruszka Katarzyna , Bielewicz Dawid , Karlowski Wojciech , Nuc Przemyslaw , Szweykowska-Kulinska Zofia , Pacak Andrzej
Published: February 2020

Background: Small RNAs (sRNAs) are 18–24 nt regulatory elements which are responsible for plant development regulation and participate in many plant stress responses. Insufficient inorganic phosphate (Pi) concentration triggers plant responses to balance the internal Pi level. Results: In this study, we describe Pi-starvation-responsive small RNAs and transcriptome changes in barley (Hordeum vulgare L.) using Next-Generation Sequencing (NGS) data derived from three different types of NGS libraries: (i) small RNAs, (ii) degraded RNAs, and (iii) functional mRNAs. We find that differentially and significantly expressed miRNAs (DEMs, p-value < 0.05) are represented by 162 (44.88 % of total differentially expressed small RNAs) molecules in shoot and 138 (7.14 %) in root; mainly various miR399 and miR827 isomiRs. The remaining small RNAs (i.e., those without perfect match to reference sequences deposited in miRBase) are considered as differentially expressed other sRNAs (DESs, Bonferroni correction). In roots, a more abundant and diverse set of other sRNAs (1796 unique sequences, 0.13 % from total unique reads obtained under low-Pi) contributes more to the compensation of low-Pi stress than that in shoots (199 unique sequences, 0.01 %). More than 80 % of differentially expressed other sRNAs are upregulated in both organs. Additionally, in barley shoots, upregulation of small RNAs is accompanied by strong induction of two nucleases (S1/P1 endonuclease and 3’-5’ exonuclease). This suggests that most small RNAs may be generated upon endonucleolytic cleavage to increase the internal Pi pool. Transcriptomic profiling of Pi-starved barley shoots identify 98 differentially expressed genes (DEGs). A majority of the DEGs possess characteristic Pi-responsive cis-regulatory elements (P1BS and/or PHO element), located mostly in the proximal promoter regions. GO analysis shows that the discovered DEGs primarily alter plant defense, plant stress response, nutrient mobilization, or pathways involved in the gathering and recycling of phosphorus from organic pools.Conclusions: Our results provide comprehensive data to demonstrate complex responses at the RNA level in barley to maintain Pi homeostasis and indicate that barley adapts to Pi scarcity through elicitation of RNA degradation. Novel P-responsive genes were selected as putative candidates to overcome low-Pi stress in barley plants.
RNA-sequencing reveals positional memory of multipotent mesenchymal stromal cells from oral and maxillofacial tissue transcriptomes
Authors: Onizuka Satoru , Yamazaki Yasuharu , Park Sung-Joon , Sugimoto Takayuki , Sone Yumiko , Sjöqvist Sebastian , Usui Michihiko , Takeda Akira , Nakai Kenta , Nakashima Keisuke , Iwata Takanori
Published: February 2020

Background Multipotent mesenchymal stromal cells (MSCs) can be isolated from numerous tissues and are attractive candidates for therapeutic clinical applications due to their immunomodulatory and pro-regenerative capacity. Although the minimum criteria for defining MSCs have been defined, their characteristics are known to vary depending on their tissue of origin.Results We isolated and characterized human MSCs from three different bones (ilium (I-MSCs), maxilla (Mx-MSCs) and mandibular (Md-MSCs)) and proceeded with next generation RNA-sequencing. Furthermore, to investigate the gene expression profiles among other cell types, we obtained RNA-seq data of human embryonic stem cells (ESCs) and several types of MSCs (periodontal ligament-derived MSCs, bone marrow-derived MSCs, and ESCs-derived MSCs) from the Sequence Reads Archive and analyze the transcriptome profile. We found that MSCs derived from tissues of the maxillofacial region, such as the jaw bone and periodontal ligament, were HOX-negative, while those derived from other tissues were HOX-positive. We also identified that MSX1, LHX8, and BARX1 , an essential regulator of craniofacial development, were strongly expressed in maxillofacial tissue-derived MSCs. Although MSCs may be divided into two distinct groups, the cells originated from over the neck or not, on the basis of differences in gene expression profile, the expression patterns of all CD antigen genes were similar among different type of MSCs, except for ESCs.Conclusions Our findings suggest that MSCs from different anatomical locations, despite meeting general characterization criteria, have remarkable differences in gene expression and positional memory. Although stromal cells from different anatomical sources are generally categorized as MSCs, their differentiation potential and biological functions vary. We suggested that MSCs may retain an original tissue memory about the developmental process, including gene expression profiles. This could have an important impact when choosing an appropriate cell source for regenerative therapy using MSCs.
High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid
Authors: Zhang Xue , Du Wenmei , Zhang Junjie , Zou Zhen , Ruan Changchun
Published: February 2020

Background The parasitoid insect Trichogramma dendrolimi can successfully enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi. Previous studies on diapause have focused mostly on ecological characteristics, so the molecular mechanism of diapause in T. dendrolimi is mostly unknown. In this study, we compared transcriptomes of diapause and non-diapause T. dendrolimi to identify genes involved in the development of diapause. Results Transcriptome sequencing was performed using different samples, including diapause prepupae, pupae after diapause, normal prepupae, and pupae. This initially yielded a total of 87,022 unigenes with an average length of 1,604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7,593 sequences with an average length of 3351 bp. Among the differentially expressed genes, 1,204 were specifically expressed during the diapause stage, and 820 were significantly up-regulated. The result of GO enrichment analysis revealed that binding, oxidation−reduction process, and ribosome biogenesis were significantly affected. Ten genes were selected for validation using a quantitative real-time PCR (qPCR). The changes showed the same trend between the qPCR and RNA-Seq results. Based on previous studies and our data, several genes were identified to be involved in diapause, including UDP-glucuronosyltransferase; Glutathione-S-transferase, ATGs, zinc finger protein genes, cell cycle-related genes, Trehalase, trehalose transporter, hsp68, p53, and DNA damage-regulated gene 1 (pdrg1), and genes related to lipid metabolism were also included. Conclusions In this study, we generated a great amount of transcriptome data from T. dendrolimi, providing a resource for gene function research. The diapause-related genes that we identified establish a valuable basis for future studies on the molecular mechanisms of diapause, not only for T. dendrolimi, but for other species as well.
Genome-wide identification and expression analysis of WRKY transcription factors in pearl millet (Pennisetum glaucum) under dehydration and salinity stress.
Authors: Chanwala Jeky , Satpati Suresh , Dixit Anshuman , Parida Ajay , Giri Mrunmay Kumar , Dey Nrisingha
Published: February 2020

Background: Plants have developed various sophisticated mechanisms to cope up with climate extremes and different stress conditions, especially by involving specific transcription factors (TFs). The members of the WRKY TF family are well known for their role in plant development, phytohormone signaling and developing resistance against biotic or abiotic stresses. In this study, we performed a genome-wide screening to identify and analyze the WRKY TFs in pearl millet ( Pennisetum glaucum; PgWRKY ) , which is one of the most widely grown cereal crop in the semi-arid regions. Results: A total number of 97 putative PgWRKY proteins were identified and classified into three major Groups (I-III) based on the presence of WRKY DNA binding domain and zinc-finger motif structures. Members of Group II have been further subdivided into five subgroups (IIa-IIe) based on the phylogenetic analysis. In-silico analysis of PgWRKYs revealed the presence of various cis-regulatory elements in their promoter region like ABRE, DRE, ERE, EIRE, Dof, AUXRR, G-box, etc., suggesting their probable involvement in growth, development and stress responses of pearl millet. Chromosomal mapping evidenced uneven distribution of identified 97 PgWRKY genes across all the seven chromosomes of pearl millet. Synteny analysis of PgWRKYs established their orthologous and paralogous relationship among the WRKY gene family of Arabidopsis thaliana, Oryza sativa and Setaria italica . Gene ontology (GO) annotation functionally categorized these PgWRKYs under cellular components, molecular functions and biological processes. Further, the differential expression pattern of PgWRKY s was noticed in different tissues (leaf, stem, root) and under both drought and salt stress conditions. The expression pattern of PgWRKY33 , PgWRKY62 and PgWRKY65 indicates their probable involvement in both dehydration and salinity stress responses in pearl millet. Conclusion: Functional characterization of identified PgWRKY s can be useful in delineating their role behind the natural stress tolerance of pearl millet against harsh environmental conditions. Further, these PgWRKY s can be employed in genome editing for millet crop improvement. Keywords : Pearl millet, WRKY Transcription Factors , cis-regulatory elements, Synteny, Abiotic stress
MiR-3613-3p from carcinoma associated fibroblasts exosomes promoted breast cancer cell proliferation and metastasis by regulating SOCS2 expression
Authors: Liu Yonglei , Yang Yanfei , Du Junxian , Lin Dong , Li Feng
Published: February 2020

Background Exosomes carrying microRNA (miRNAs) mediate cell to cell communication which are important regulators in cancer growth and progression. However, the roles and molecular mechanism of the miRNAs in the exosomes from carcinoma associated fibroblasts (CAFs) are still not clear. Methods The targeted gene of miR-3613-3p was predicted by TargetScan and miRanda. The proliferation of cancer cells was conducted by cell counting kit-8 and colony formation assay. Cancer cell migration and invasion were measured by wound healing assay and Transwell assays respectively. Luciferase activity was assayed by dual luciferase assay system. miRNA and mRNA expression was measured by real time RT-PCR. SOCS2 protein levels was assayed by western blotting. Results It was verified that miR-3613-3p was up-regulated in exosomes from fibroblasts educated by TGF-β1, breast cancer cells and breast cancer tissues. Exosomal miR-3613-3p promoted breast cancer cell proliferation and metastasis. Loss-of-function experiments revealed that miR-3613-3p down-regulation in the CAFs exosomes suppressed cell proliferation and drug resistance in breast cancer by targeting SOCS2 expression. The clinical data showed that miR-3613-3p was negatively related to SOCS2 in breast cancer tissues. Conclusion These findings demonstrated that activated fibroblasts exosomes with high levels of miR-3613-3p played an oncogenic role in breast cancer cell survival, metastasis and drug responses, which suggested that the oncogenic role of miR-3613-3p in breast cancer progression.
Whole blood transcriptomic investigation identifies long non-coding RNAs as regulators in sepsis
Authors: Cheng Lixin , Nan Chuanchuan , Kang Lin , Zhang Ning , Liu Sheng , Chen Huaisheng , Hong Chengying , Chen Youlian , Liang Zhen , Liu Xueyan
Published: February 2020

Background: Sepsis is a fatal disease referring to the presence of a known or strongly suspected infection coupled with systemic and uncontrolled immune activation causing multiple organ failure. However, neither pathogenic long non-coding RNAs (lncRNAs) nor biological network analysis in sepsis draws enough attention to the society of sepsis studies. Methods: We performed an in-silico investigation of the gene coexpression pattern for the patients response to all-cause sepsis in consecutive intensive care unit (ICU) admissions. Sepsis coexpression gene modules were identified using WGCNA and enrichment analysis. lncRNAs were determined as sepsis biomarkers based on the interactions among lncRNAs and the identified modules. Results: Twenty-three sepsis modules, including both differentially expressed modules and prognostic modules, were identified from the whole blood RNA expression profilings of sepsis patients. Five lncRNAs, FENDRR, MALAT1, TUG1, CRNDE, and ANCR, were detected as sepsis regulators based on the interactions among lncRNAs and the identified coexpression modules. Furthermore, we found that CRNDE and MALAT1 may act as miRNA sponges of sepsis related miRNAs to regulate the expression of sepsis modules. Ultimately, FENDRR, MALAT1, TUG1, and CRNDE were reannotated using three independent lncRNA expression datasets and validated as differentially expressed lncRNAs. Conclusion: The procedure facilitates the identification of prognostic biomarkers and novel therapeutic strategies of sepsis. Our findings highlight the importance of transcriptome modularity and regulatory lncRNAs in the progress of sepsis.
PREDIRA (PRediction mEdical DevIce for Rheumatoid Arthritis): Scale-up of unique predictive online platform highly improving the quality of life of Rheumatoid Arthritis’ patient by personalized and efficient biotherapies prescription
Authors: Nuñez Dalifer Freites , Baillet Athan , Rodriguez-Rodriguez Luis , Nguyen Minh Vu Chuong , Gonzalez Isidoro , Pablos Jose Luis , Balsa Alejandro , Vazquez Monica , Gaudin Philippe , Fernandez-Gutierrez Benjamín
Published: February 2020

Background: Rheumatoid arthritis (RA) is one of the leading chronic inflammatory rheumatism. First-line therapy with synthetic disease modifying anti-rheumatic drugs (sDMARD) is insufficiently effective in 40% of cases and these patients are treated with biotherapies. The increases use of these drugs each year is becoming a public health issue with considerable economic burden. This cost is 20 times higher than that of sDMARD. However, among patients treated with biotherapies, clinical practice shows that about one-third will not respond to the selected drug. In non-response cases, practitioners currently have no choice but to perform an empirical switching between different treatments, because no tool capable of predicting the response or non-response to these molecules is currently available.Methods : The study is a prospective, phase III, controlled, multicenter, and randomized, single-blind (patient) clinical trial, including RA patients with a previous failure to anti-TNF therapies. The main objective is the analysis of the clinical and pharmacoeconomic impact after 6 months of treatment. Intervention arm: Prescription of biotherapy (rituximab, adalimumab, abatacept) using SinnoTest® software, a prediction software based on proteomic biomarkers. Control arm: Prescription of biotherapy based on current practice, without the SinnoTest® software (any biotherapy). In addition, a sub study will be carried out within this trial to generate a biobank and further analyze the proteomic profile of the patients and their modification throughout the study.Discussion : This clinical trial study will be the first validation study of a biotherapy response prediction software, bringing personalized medicine into the management of RA. We expect that the findings from this study will bring several benefits for the patient and the Health Care System. Trial registration : The study was registered at clincaltrials.gov (NCT04147026). Registered on 31 October, 2019.
Evaluation the efficacy and safety of fire needle compared to filiform needle on knee osteoarthritis: study protocol for a randomised controlled trial
Authors: Gao Yuanjie , Liu Lu , Li Bin , Guo Jing , Liu Huilin , Wang Shaosong , Zhang Fan , Ji Xu , Fu Yuanbo , Wang Yizhan , Sun Jingqing , Yuan Fang
Published: February 2020

Background: Knee osteoarthritis is a common clinical chronic degenerative disease associated with high morbidity and long-term disability. Previous studies have confirmed the efficacy of acupuncture on knee osteoarthritis. Fire needle acupuncture is a combination of heat and acupuncture, which may be more effective than the commonly used filiform needle acupuncture. This study is designed as a randomized controlled trial to evaluate the efficacy and safety of fire needle acupuncture compared to filiform needle acupuncture in knee osteoarthritis patients. Methods and analysis: A total of 100 participants will be randomly assigned to two different groups. Participants will receive fire-needle acupuncture treatment in the fire needle group, while participants in the filiform needle group will be treated with a filiform needle at the same acupuncture points as the fire needle group. All participants will receive a 6-weeks of treatment (2 times per week). The primary outcome is the change of the Western Ontario and McMaster Universities Osteoarthritis Index, and the secondary outcomes include the change of the Visual Analogue Scale, and 12-item Short Form Health Survey from baseline to endpoint. Ethics and dissemination: Ethical approval of this study was granted by the Research Ethical Committee of Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University (2018SB-066). Written informed consent will be obtained from all participants. Outcomes of the trial will be disseminated through peer-reviewed publications. Trial registration number: ChiCTR1800019579
Effects of perioperative ERAS pathway management VS traditional management on clinical outcomes in laparoscopic-assisted radical resection of distal gastric cancer The GISSG18-01 Randomized Clinical Trial
Authors: Tian Yulong , Cao Shougen , Li Leping , He Qingsi , Xia Lijian , Jiang Lixin , Ding Yinlu , Wang Xinjian , Wang Hao , Mao Weizheng , Hui Xizeng , Shi Yiran , Zhang Huanhu , Chu Xianqun , Kehlet Henrik , Zhou Yanbing
Published: February 2020

Background: As well known, the incidence of gastric cancer in East Asian countries is much higher than the international average. Therefore, improving the prognosis of patients and establishing effective clinical pathways are important topics for the prevention and treatment of gastric cancer. At present, the enhanced recovery after surgery (ERAS) pathway is widely used in the field of gastric surgery. Many RCT studies have proven that the ERAS regimen can not improve the short-term clinical outcomes of gastric cancer patients. However, a prospective study on the effect of the ERAS pathway on the prognosis of gastric cancer patients has not been reported. This trial aims to confirm whether ERAS pathway can improve disease-free survival (DFS) and overall survival (OS) in patients undergoing laparoscopic-assisted radical resection for distal gastric cancer.Methods/design: This study is a prospective, multicenter, randomized controlled trial (RCT). This experiment will include randomly divided groups, the experimental group and the control group, according to a proportion of 1:1. The perioperative period of the experimental group will be managed according to the ERAS pathway, and the control group will be managed according to the traditional management mode. An estimated 400 patients will be enrolled. The main endpoint is to compare the 3-year OS and PFS between the two groups.Discussion: This RCT should demonstrate whether ERAS pathway is superior to traditional treatment on inflammatory indexes, short-term clinical outcome and survival for laparoscopic assisted radical resection of distal gastric cancer.Our data can provide evidence that the ERAS pathway improves survival in patients with gastric cancer.Trial registration: Chinese Clinical Trial Registry, CHiCTR1900022438. Registered on 11 April 2019
Is immediate weight bearing safe for lateral malleolus fracture after surgery?
Authors: Lee Sang-June , Choi Young Rak , Choi SeongJu , Lee Ho Seong , Park Seung-Hwan
Published: February 2020

Introduction Lateral malleolus fracture is one of the most common fractures. However, there is controversy regarding the rehabilitation protocols used after surgery. In particular, the initiation point for weight bearing has not been standardized. In the present study, we investigated the prognostic difference between immediate and delayed weight bearing on lateral malleolus fractures. Materials and Methods The medical records of matched 50 and 41 patients in the immediate and delayed weight-bearing groups were reviewed retrospectively. All patients were treated with open reduction and internal fixation using an anatomical locking compression plate. In the immediate weight-bearing group (IWB), tolerable weight bearing (i.e., can be endured immediately after surgery with crutches) was permitted. In the delayed weight-bearing group (DWB), weight bearing was completely restricted for 4 weeks after surgery. Ankle motion exercise was permitted in both groups starting from the day after surgery. Radiographic assessment data and clinical outcomes were reviewed. Results No significant differences in the radiographic assessments and complications were found between the two groups. Significant differences in shortened length of hospital stay and time to return to work with the IWB rehabilitation protocol were confirmed (IWB group vs. DWB group: 5.7 vs. 8.0 days and 6.0 vs. 8.0 months, respectively). A significant difference in sport factor was observed in the Foot and Ankle Outcome Score at 3 months postoperatively (75.5 vs 68.5). Conclusions We found no significant differences between the two groups with respect to postoperative radiological outcome and complications. The benefits of shortening the time to return to work and length of hospital stay associated with the IWB rehabilitation protocol were confirmed. In conclusion, immediate weight bearing is recommended in patients with lateral malleolus fracture after anatomical reduction and firm fixation by surgery.
Diagnostıc Performance Of Erythropoetin And Erythropoetin Receptor Levels In Patients With Generalized Anxiety Disorder
Authors: Kurutas Ergul Belge
Published: February 2020

Background Generalized anxiety disorder (GAD) is a prevalent psychiatric disorder. Diagnosis of GAD depends on subjective complaints of patients, thus the need for biological markers is constantly emerging. This study aimed, for the first time, to investigate diagnostic value of Erythropoietin (Epo) and its receptor (EpoR) levels in drug-naive patients with GAD. Methods This study included 45 newly diagnosed drug-naive patients with GAD, aged and gender-matched 30 healthy controls. Medical histories were obtained, and physical examinations and laboratory tests were conducted; the Hamilton Anxiety Rating Scale (HAM-A) was also used for all participants. Serum Epo and EpoR levels were measured by ELISA. Results HAM-A score was significantly higher in GAD patients versus the controls (p< 0.05). While the levels of Epo in patients with GAD were lower than the control patients, EpoR levels were increased in these patients (p<0.05). Epo/EpoR ratios were significantly lower in the patients with GAD than in the control subjects (p<0.05). A positive significant correlation was observed between the EpoR level and the HAM-A score (r= 0.755, p<0.001). However, there was a negative significant correlation between Epo levels and HAM-A score (r= -0.749, p<0.001). ROC analysis revealed both sensitivity and specificity of 100%, respectively, for the presence of anxiety when the serum Epo value was 7.64 ng/mL (the area under the curve was 1.000) and EpoR value was 0.97 ng/mL (the area under the curve was 1.000). Conclusion Our findings showed that Epo/EpoR ratio may be an important biomarker for GAD. Epo may be a good choice for GAD treatment.
Clinical Implications of 37 Childhood Cases with 2019-nCoV Infection in Shenzhen, China
Authors: Wang Yanrong , LIU Yingxia , Yang Yang , WANG Xianfeng , LIU Lei
Published: February 2020

Background 2019-nCoV was first identified in December 2019 in samples obtained from a 61 years old man who died of acute respiratory failure in the city of Wuhan, China with a subsequent outbreak in China. Till now, there were rare reports about childhood patients with 2019-nCoV infection.Methods We report 37 children diagnosed with 2019-nCoV infection admitted in the Third People’s hospital of Shenzhen from December 11, 2019 to February 11, 2020.Results There were 9 mild cases, 20 common cases and 1 severe case and 7 cases with asymptomatic infection. The age ranged from 7 months to 18 years old and the median age was 7 years old. The common clinical features were fever (29.7%, 11/37) and cough (32.4%, 12/37). No death occurred among our patients. 86.5% (32/37) children were infected with 2019-nCoV after their family members (parents or grandparents). 1 child was identified with 2019-nCoV infection after 3 times testing. The majority (78.4%) of cases occurred in those children who travelled to Hubei Province. The percentage of cases in preschool and in schools was 21.6% and 54.1% (P<0.05) respectively.Conclusions Family cluster transmission of 2019-nCoV was suspected in 86.5% of patients. Timely continuous 2019-nCoV pathogen testing is recommended. More attention should be paid to school children.
Molecular assessment of kelch13 non-synonymous mutations in Plasmodium falciparum isolates from Central African Republic (2017–2019)
Authors: Nzoumbou-Boko Romaric , Panté-Wockama Chris-Boris Gildas , Ngoagoni Carine , Petiot Nathalie , Legrand Eric , Vickos Ulrich , Gody Jean-Chrysostome , Manirakiza Alexandre , Ndoua Christophe , Lombard Jean-Pierre , Menard Didier
Published: February 2020

Background: Over the last decade, Artemisinin-based Combination Therapies (ACT) have contributed substantially to the decrease in malaria-related morbidity and mortality. The emergence of Plasmodium falciparum parasites resistant to artemisinin derivatives in Southeast Asia and the risk of their spread or of local emergence in sub-Saharan Africa are a major threat to public health. This study thus set out to estimate the proportion of P. falciparum isolates, with PfKelch13 gene mutations associated with artemisinin resistance previously detected in Southeast Asia. Methods: Blood samples were collected in two sites of Bangui, the capital of the Central African Republic form 2017 to 2019. DNA was extracted and nested PCR were carried out to detect Plasmodium species and mutations in the propeller domain of the PfKelch13 gene. Results: A total of 255 P. falciparum isolates were analyzed. Among them, P. ovale DNA was found in four samples (1.57%, 4/255). Of 187 samples with interpretable PfKelch13 sequences, four isolates presented a mutation in the PfKelch13 gene (2.1%, 4/187), including one non-synonymous mutation (Y653N) (0.5%, 1/187). This mutation has never been described as associated with artemisinin resistance in Southeast Asia and its in vitro phenotype is unknown. Conclusion: This preliminary study indicates the need for a larger study on samples collected across the whole country along with the evaluation of in vitro and in vivo phenotype profiles of PfKelch13 mutant parasites to estimate the risk of artemisinin resistance in the CAR.
Sex differences in the relationship between dietary pattern adherence and cognitive function among older adults: Findings from the NuAge study
Authors: D’Amico Danielle , Parrott Matthew D. , Greenwood Carol E. , Ferland Guylaine , Gaudreau Pierrette , Belleville Sylvie , Laurin Danielle , Anderson Nicole D. , Kergoat Marie-Jeanne , Morais Jose A. , Presse Nancy , Fiocco Alexandra J.
Published: February 2020

Background Consumption of a prudent dietary pattern rich in healthy nutrients is associated with enhanced cognitive performance in older adulthood, while a Western dietary pattern low in healthy nutrients is associated with poor age-related cognitive function. Sex differences exist in dietary intake among older adults; however, there is a paucity of research examining the relationship between sex-specific dietary patterns and cognitive function in later life. Methods The current study aimed to investigate sex differences in the relationship between sex-specific dietary pattern adherence and global cognitive function at baseline and over a 3-year follow-up in 1268 community-dwelling older adults ( M age =74 years, 52% women) from the Quebec Longitudinal Study on Nutrition and Successful Aging (NuAge). A Food Frequency Questionnaire was used to estimate dietary intake. Sex-specific dietary pattern scores were derived using principal component analysis. Global cognition was assessed using the Modified Mini-Mental State Examination (3MS). Results Adjusted linear mixed effects models indicated that a healthy, prudent dietary pattern was not associated with baseline cognitive performance in men or women. No relationship was found between Western dietary pattern adherence and baseline cognitive function in women. Among men, adherence to an unhealthy, Western dietary pattern was associated with poorer baseline cognitive function ( β = -0.652, p = 0.02). No association was found between prudent or Western dietary patterns and cognitive change over time in men or women. Conclusions These findings highlight the importance of conducting sex-based analyses in aging research and suggest that the relationship between dietary pattern adherence and cognitive function in late life may be sex-dependent.
Comparison of Childbirth Experiences and Postpartum Depression in Three Groups of Primiparous Women with no Participation, Irregular Participation and Regular Participation in Childbirth Preparation Classes
Authors: Hassanzadeh Robab , Abbas-Alizadeh Fateme , Meedya Shahla , Charandabi Sakineh Mohammad-Alizadeh , Mirghafourvand Mojgan
Published: February 2020

Background Assessment of women’s childbirth experience is an important indicator of maternal care systems. Positive childbirth experiences improve mothers’ health, whereas negative childbirth experiences can cause psychological stresses and, in extreme cases, may lead to postpartum depression. This study compared childbirth experiences and postpartum depression levels in three groups of primiparous women with no participation, irregular participation, and regular participation in childbirth preparation classes. Methods In this cohort study, 204 primiparous women at 35-37 weeks of gestation were selected using cluster sampling from the health centers of Tabriz, Iran. The participants were assigned to three groups of no participation, irregular participation (attending 1-3 sessions), and regular participation (attending 4-8 sessions) in childbirth preparation classes. Interviews were conducted one month after delivery to complete the Childbirth Experience Questionnaire (CEQ) and Edinburgh Postnatal Depression Scale (EPDS). The general linear model (GLM) was used to compare childbirth experience and postpartum depression among groups. Results Based on the general linear model, the mean score of childbirth experience in women in the regular participation group was significantly higher than those of women in irregular participation (P= 0.032) and in no participation (P<0.001) groups. In addition, the mean score of postpartum depression was significantly lower in women in the regular participation group than that of the women in the no participation group (P<0.001). However, no significant difference was found between the regular and irregular participation groups in terms of postpartum depression levels (P= 0.257). Conclusion Prenatal training classes improved childbirth experience and reduced postpartum depression levels. These training classes seem to improve mothers’ health by increasing their knowledge and awareness of their abilities to endure labor pain. Ethical Code IR.TBZMED.REC.1398.066
Anchor free object detection method using mask optimization
Authors: Fan Beibei , Yang HE , Guo Ling ling
Published: February 2020

The anchor-free method based on key point detection has made great progress. However, the anchor-free method is too dependent on using a convolutional network to generate a rough heat map. It is difficult for the network to detect objects whose shape changes greatly, and small objects are difficult to detect. In order to solve this problem, first of all, we use the most advanced mask attention mechanism algorithm in the network to increase the accuracy of the thermodynamic generation of network detection. Then, We also designed an optimized fire model to reduce the size of the model. The masking mechanism optimizes the feature map of the network to enhance the detection capability in network space and improve the accuracy of heatmaps generation. Our approach achieved an accuracy of 91.84% and a recall 89.83% in the Tencent-100K dataset. Our approach is also competitive with the most advanced approach.
Prevalence and risk factors for bone loss in Southern Chinese with rheumatic diseases
Authors: Hu Zhuoran , Xu Shuiming , Yang Qingyuan , Qi Jun , Lin He , Ni Weifeng , Du Keqian , Gu Jieruo , Lin Zhiming
Published: February 2020

Objectives: This study was to explore the prevalence of different severities of bone loss and the potential risk factors in rheumatic patients.
Method: An analytical cross-sectional study design was performed. For the present study, 1,398 rheumatic patients and 302 healthy subjects were recruited and further accepted for data collection and blood and bone mineral density tests. Risk factors for bone loss in rheumatic patients were further analyzed by using multivariate logistic regression analysis.
Results: (1) Rheumatic patients in this study are consisted of 40.0% rheumatoid arthritis, 14.7% systemic lupus erythematosus, 14.2% osteoarthritis, 9.2% ankylosing spondylosis, 7.9% gout, 7.0% primary Sjogren syndrome, 3.8% systemic sclerosis, and 3.2% mixed connective tissue disease. (2) In male patients aged under 50 and premenopausal female patients, the bone mineral density score of patients is lower than the healthy persons with the same gender and age (34.3% vs 18.2%， P =0.045). (3) Osteopenia and osteoporosis are more prevailing in male patients aged or older than 50 and postmenopausal female patients than healthy counterparts (92.7% vs 87.2%, P =0.017). (4) Regression analysis indicated that compared with a healthy population, those with systemic lupus erythematosus, rheumatoid arthritis and ankylosing spondylosis gained the highest odd ratio of ‘score below the expected range for age’, osteopenia and osteoporosis, respectively. (5) Hypovitaminosis D, ‘CRP elevated’ was commonly-associated with impaired bone mineral density in all rheumatic patients.
Conclusion: Bone loss commonly occurs at any age in the setting of rheumatic diseases. Hypovitaminosis D, elevated CRP was probably commonly-associated with impaired BMD in all age groups of rheumatic patients.
Distal radial cortical bone thickness correlates with bone mineral density and can predict osteoporosis: a cohort study
Authors: Ye ChunXiao , Guo YingBin , Zheng YouHui , Wu ZhenBin , Chen KaiYu , Zhang XiaoLing , Xiao LiangXiu , Chen ZhiMing
Published: February 2020

Background We evaluated the correlation of the bone mineral density (BMD) of the hip and lumbar spine with the distal radius cortical thickness (DRCT) measured on anteroposterior radiographs to establish a method for predicting osteoporosis. Methods We assessed 147 patients aged ≥50 years with distal radius fractures who underwent wrist radiographs and dual-energy X-ray absorptiometry. The DRCT was measured and calculated at two levels of the distal radius of the injured wrist on the radiographs. Results In the Pearson correlation and simple linear regression analyses, the DRCT was positively correlated with hip BMD (r = 0.393, P < 0.01) and lumbar spine BMD (r = 0.529, P < 0.01). Each 1-mm increase in DRCT was associated with a 0.051-g/cm 2 increase in hip BMD (R 2 = 0.154, P < 0.01) and a 0.080-g/cm 2 increase in lumbar spine BMD (R 2 = 0.280, P < 0.01). A DRCT of 5.1 mm was selected as the cutoff point for predicting osteoporosis, with the highest Youden index of 0.560, 83.3% sensitivity, 72.7% specificity, and a 66.7% negative predictive value. Conclusion Cortical thickness measurements obtained from anteroposterior wrist radiographs were positively correlated with hip and lumbar spine BMD measurements. This technique is suggested as a rapid, inexpensive, and sensitive method for predicting osteoporosis.
Existence of Hepatitis B Surface Protein Mutations and Other Variants: Demand for Hepatitis B Infection Control in Cambodia
Authors: Ko Ko , Takahashi Kazuaki , Nagashima Shintaro , Yamamoto Chikako , Vichit Ork , Sugiyama Aya , Akita Tomoyuki , Ohisa Masayuki , Chuon Channarena , Hoss Shafiqul Md , Mao Bunsoth , Tanaka Junko
Published: February 2020

Aims This study aimed to detect Hepatitis B virus (HBV) genome sequences and their variants as of nationwide scale using dried blood spot (DBS) samples and to provide up-to-date reference data for infection control and surveillance in Cambodia. Method Among 2,518 children age 5-7 years and their 2,023 mothers participated in 2017 Cambodia nationwide sero-survey on hepatitis B surface antigen (HBsAg) prevalence using multistage random sampling strategy, 95 mothers and 13 children positive to HBsAg were included in this study. HBV DNA was extracted from DBS, then performed polymerase chain reaction. HBV genotypes and potential variants were examined by partial and full length genomic analysis. Results HBV DNA positive rate was 3.46% (70/2023) in mothers and 0.48% (12/2518) in their children. Genotype C (80.49%) was abundantly found throughout the whole Cambodia whilst genotype B (19.51%) was exclusively found in regions bordering Vietnam. HBs mutants were found in 24.29% of mothers and 16.67% of children with HBV DNA positive sera. Full-length genome analysis revealed the homology of 99.62-100% in each mother-child pair. Genotype B was clarified to recombinant genotype B4/C2 and B2/C2. Double (48.39%) and combination mutation (32.26%) were observed in core promoter region of HBV C1 strains. Conclusion This study showed the capable of DBS for large-scale molecular epidemiological study of HBV in resource limited countries. Full-genome sequences yield the better understanding of sub-genotypes, their variants and the degree of homology between strains isolated from mother-child pairs calls for effective strategies on prevention, control and surveillance of mother-to-child HBV transmission in Cambodia.
Effect of isospin averaging for $ppK^-$ kaonic cluster
Authors: Vlahovic Branislav , Filikhin Igor
Published: February 2020

The kaonic cluster ppK^-ppK−
is described by isospin-dependent N{\bar K}NK‾
potentials with significant difference between singlet and triplet
components. The quasi-bound state energy of the system is calculated
based on the configuration space Faddeev equations within isospin and
averaged potential models. The isospin averaging of
N{\bar K}NK‾
potentials is used to simplify the isospin model to isospinless one. We
show that three-body bound state energy E_{3}E3
has a lower bound within the isospin formalism due to relation
\left\vert E_{3}(V_{NN}=0)\right\vert<2\left\vert E_{2}\right\vert|E3(VNN=0)|<2|E2|,
where E_{2}E2
is the binding energy of isospin singlet state of the
N{\bar K}NK‾
subsystem. The averaged potential model demonstrates opposite relation
between |E_{2}||E2|
and |E_{3}(V_{NN}=0)||E3(VNN=0)|.
An accurate allowance for initial and final state interactions in the treatment of the alpha-alpha Bremsstrahlung
Authors: Arslanaliev Adam , Shebeko Aleksandr V.
Published: February 2020

The alpha-alpha bremsstrahlung is studied using the generalization of
the Siegert theorem. The corresponding amplitude is written in the gauge
invariant form. Special attention is paid to taking into account the
Coulomb interaction. Some correlation function is found and its
dependence on the strong alpha-alpha interaction is discussed.
QED in the clothed-particle representation: a fresh look at positronium properties treatment
Authors: Kostylenko Yan , Arslanaliev Adam , Shebeko Aleksandr V.
Published: February 2020

We have extended our previous applications of the method of unitary
clothing transformations (UCTs) in mesodynamics [1,2] to quantum
electrodynamics (QED) [3,4]. An analytical expression for the QED
Hamiltonian in the clothed-particle representation (CPR) has been
derived. Its distinctive feature is the appearance of a new family of
the Hermitian and energy independent interaction operators built up in
the e^2e2-order
for the clothed electrons and positrons instead the primary canonical
interaction between electromagnetic and electron-positron fields. The
problem of describing the bound states in QED in case of the positronium
system has been considered. The first correction to the energy of the
ground state of the para-positronium and its decay rate to two photons
has been calculated by using the new interaction operators.
Three-nucleon force effects in the FSI configuration of the d(n, nn)p breakup reaction
Authors: Hiroyuki Kamada , Witała Henryk , Golak Jacek , Skibiński Roman
Published: February 2020

We investigated three-nucleon (3N) force effects in the final state
interaction (FSI) configuration of the d (n,nn) pd(n,nn)p
breakup reaction at the incoming nucleon energy
E_nEn=
200 MeV. Although 3N force effects for the elastic nucleon-deuteron
scattering cross section at comparable energies are located
predominantly in the region of intermediate and backward angles, the
corresponding 3N force effects for the integrated FSI configuration
breakup cross section are found also at forward scattering angles.
A time-dependent Hartree-Fock study of triple-alpha dynamics
Authors: Stevenson Paul , Willerton J. L.
Published: February 2020

Time-dependent Hartree-Fock calculations have been performed for
fusion reactions of ^44He
+ ^44He
\rightarrow→^88Be^**,
followed by ^44He
+ ^88Be^**.
Depending on the orientation of the initial state, a linear chain
vibrational state or a triangular vibration is found in
^{12}12C,
with transitions between these states observed. The vibrations of the
linear chain state and the triangular state occur at
\simeq≃9
and 4 MeV respectively.
Lattice phase shifts and mixing angles for an arbitrary number of coupled channels
Authors: Bovermann Lukas , Epelbaum Evgeny , Krebs Hermann , Lee Dean
Published: February 2020

We present a lattice method for determining scattering phase shifts
and mixing angles for the case of an arbitrary number of coupled
channels. The proposed method combines a spherical wall boundary
condition and a channel-mixing auxiliary potential to extract the
full-rank S-matrix from the radial wave functions. We consider the
scattering problem of two spin-1 bosons interacting with a test
potential involving up to four coupled channels. For this benchmark
system, the phase shifts and mixing angles are shown to agree on the
lattice and in the continuum. Our method should allow to extend previous
two-channel nuclear lattice EFT simulations to mixing of more than two
partial waves.
Organização e realização do trabalho docente
Authors: De Castro Bueno Lucimara , Bagolin Zambom Luciana
Published: February 2020

Este artigo propõe compreender como professores organizam e desenvolvem seu trabalho, diante das condições encontradas nos contextos reais. Parte da concepção da ergonomia para analisar o trabalho desenvolvido por professoras do Ensino Médio no contexto de uma escola pública. Mediante utilização de entrevista semiestruturada e observação de aulas, são descritos e analisados o tempo e espaço destinados ao planejamento docente. Foi possível evidenciar que o tempo destinado para planejamento é insuficiente, se considerarmos a diversidade de situações, de alunos e de turmas que as professoras atendem, e que elas não contam, na escola, com um ambiente que ofereça recursos e espaços adequados para tanto. Assim, essa ação costuma ser realizada na residência das professoras, ocupando parte considerável de seu período de descanso. Isso não parece ser registrado, nem considerado, nem contabilizado formalmente como trabalho. Portanto, o rótulo “horas de planejamento” acaba ocultando a variedade e a complexidade de ações que compõem o planejamento docente.
Synthesis of Multi-walled Carbon Nanotubes Decorated with ZnO/Ag Nanoparticles by Co-precipitation Method
Authors: Ahmed Duha S. , Mohammed Mohammad R. , Mohammed Mustafa K.A.
Published: February 2020

Background:

This research indicated that such abstract are more informative and more
readable, in field of preparation of nanocomposite materials.

Aim:

The purpose of this work represented by decoration of Carbon Nanotubes (CNTs) with nanoparticles
using co-precipitation method and exhibit different physical properties from those of CNTs
alone and its appropriate for this particular journal.

Methods:

Preparation MWCNTs surface by Ag/ZnO nanoparticles using two steps represented by
acid treatment of MWCNTs in a mixture acid of sulfuric acid (95% H2SO4) and nitric acid (65%
HNO3) at 3:1 ratio and second step represented by decoration of Ag/ZnO nanoparticles on FMWCNTs
using co-precipitation method to prepare ZnO/Ag nanoparticles decorated on F-MWCNTs
as nanocomposite material.

Results:

The results of this work reveal the decoration of Ag-doped ZnO nanoparticles on the FMWCNTs
was achieved without any impurities as shown in XRD, Raman spectra and FTIR and
FESEM observations reveal that Ag-doped ZnO nanoparticles have spherical morphologies and adhered
on the functionalized MWCNTs, with diameters are about 22-30 nm. Also it is clear that there
were no any other materials on the surface of F-MWCNTs except ZnO and Ag which verifies the
results obtained from XRD and EDS analysis.

Conclusion:

In this article, Ag-doped ZnO nanoparticles have been successfully decorated on to modified
MWCNTs by an in-situ co-precipitation method. From XRD results, it can be seen that the (002)
peak of MWCNTs slightly increased after the addition of Ag-doped ZnO nanoparticles. Raman spectra
and FTIR analysis confirmed the formation of Zn-C or Zn-O-C bonds between acid-treated MWCNTs
and Ag-doped zinc oxide. The FESEM and EDS analysis revealed that the decoration of Ag-doped ZnO
nanoparticles on the F-MWCNTs was achieved without any impurities. Finally, the small sizes Agdoped
ZnO NPs deposited on F-MWCNTs make it very promising for biomedicine applications.
Membaca dan Menulis Permulaan Untuk Anak Usia Dini
Authors: Christianti Martha
Published: February 2020

Tujuan pengembangan bahasa untuk anak usia dini adalah agar anak mampu berkomunikasi dengan orang lain. Komunikasi tersebut dapat dilakukan dengan berbagai cara seperti membaca dan menulis. Dengan demikian, belajar membaca dan menulis sudah dapat diawali sejak usia dini yaitu kesadaran literasi dan eksplorasi dengan lingkungan. Ada beberapa kesiapan yang mempengaruhi anak untuk dapat membaca yaitu kesiapan fisik, kesiapan perceptual, kesiapan kognitif, kesiapan linguistik, kesiapan afektif, dan kesiapan lingkungan. Beberapa kegiatan bermain dapat dirancang pendidik untuk mengembangkan membaca dan menulis permulaan anak disesuaikan dengan tahapan membaca dan menulis dari para ahli. Kata Kunci: baca-tulis permulaan
KAJIAN LITERATUR PERKEMBANGAN PENGETAHUAN FONETIK PADA ANAK USIA DINI
Authors: Christianti Martha
Published: February 2020

Artikel ini merupakan kajian literatur perkembangan pengetahuan fonetik pada anak. Fonetik berkaitan dengan bunyi bahasa dan simbol. Perkembangan fonetik pada anak terjadi sangat alami pada kemampuan bahasa reseptif dan produksi bahasa. Tahap perkembangan pengetahuan fonetikpada anak terbagi dalam tahap bayi, batita, prasekolah dan taman kanak-kanak. Setiap tahap perkembangannya menunjukkan karakteristik yang khusus. Pengetahuan tentang perkembangan pengetahuan fonetik berguna bagi guru dalam menstimulasi fonetik anak dan bagi peneliti untuk melakukan penelitian terhadap fonetik anak dalam konteks budaya Indonesia. Kata kunci: perkembangan pengetahuan fonetik