bluuebunny/crossref_metadata_2025 huggingface dataset:
The Health Effects of Dietary Nitrate on Sarcopenia Development: Prospective Evidence from the UK Biobank
Authors: Na Jigen , Tan Yuefeng , Zhang Yanan , Na Xiaona , Shi Xiaojin , Yang Celi , Li Zhihui , Ji John S. , Zhao Ai
Published: December 2024

Nitrate is abundant in natural foods, especially plant-based foods, having the potential to enhance muscle function. However, its relationship with sarcopenia in the context of daily diet remains unexplored. This cohort study investigated the associations between dietary nitrate intake and sarcopenia, as well as related symptoms, using data including 28,229 participants with a mean follow-up of 9.37 years from the UK Biobank. Dietary nitrate intake was estimated using a comprehensive nitrate food database. Adjusted logistic regression models suggested potential inverse associations between total nitrate intake and risks of sarcopenia plus pre-sarcopenia (Sarc-Presarc), low hand grip strength (HGS), and low walking pace. Similar results were primarily observed for nitrate from plant-based foods. With higher intake, females appeared to have higher HGS and a decreased risk of Sarc-Presarc, while males exhibited a reduced risk of low walking pace. The inverse association between nitrate intake and low skeletal muscle mass index was more evident in individuals aged 65 and above. These associations seemed independent of antioxidants, though higher antioxidants might augment the protective effect against low walking pace. Mediation analyses indicated that protein homeostasis and blood pressure might mediate these associations. These findings suggested that a higher dietary nitrate intake from plant-based foods could contribute to sarcopenia prevention, though further research is needed to confirm these observations.
Comprehensive Review of Biological Functions and Therapeutic Potential of Perilla Seed Meal Proteins and Peptides
Authors: Hu Yangfan , Luo Huan , Netala Vasudeva Reddy , Li He , Zhang Zhijun , Hou Tianyu
Published: December 2024

This comprehensive review explores the biological functions of Perilla frutescens seed proteins and peptides, highlighting their significant potential for health and therapeutic applications. This review delves into the mechanisms through which perilla peptides combat oxidative stress and protect cells from oxidative damage, encompassing free radical scavenging, metal chelating, in vivo antioxidant, and cytoprotective activities. Perilla peptides exhibit robust anti-aging properties by activating the Nrf2 pathway, enhancing cellular antioxidant capacity, and supporting skin health through the promotion of keratinocyte growth, maintenance of collagen integrity, and reduction in senescent cells. Additionally, they demonstrate antidiabetic activity by inhibiting α-amylase and α-glucosidase. The cardioprotective effects of perilla peptides are underscored by ACE-inhibitory activities and combat oxidative stress through enhanced antioxidant defenses. Further, perilla peptides contribute to improved gut health by enhancing beneficial gut flora and reinforcing intestinal barriers. In liver, kidney, and testicular health, they reduce oxidative stress and apoptotic damage while normalizing electrolyte levels and protecting against cyclophosphamide-induced reproductive and endocrine disruptions by restoring hormone synthesis. Promising anticancer potential is also demonstrated by perilla peptides through the inhibition of key cancer cell lines, alongside their anti-inflammatory and immunomodulating activities. Their anti-fatigue effects enhance exercise performance and muscle function, while perilla seed peptide nanoparticles show potential for targeted drug delivery. The diverse applications of perilla peptides support their potential as functional food additives and therapeutic agents.
Current Status of Bioprinting Using Polymer Hydrogels for the Production of Vascular Grafts
Authors: Matějková Jana , Kaňoková Denisa , Matějka Roman
Published: December 2024

Cardiovascular disease is one of the leading causes of death and serious illness in Europe and worldwide. Conventional treatment—replacing the damaged blood vessel with an autologous graft—is not always affordable for the patient, so alternative approaches are being sought. One such approach is patient-specific tissue bioprinting, which allows for precise distribution of cells, material, and biochemical signals. With further developmental support, a functional replacement tissue or vessel can be created. This review provides an overview of the current state of bioprinting for vascular graft manufacturing and summarizes the hydrogels used as bioinks, the material of carriers, and the current methods of fabrication used, especially for vessels smaller than 6 mm, which are the most challenging for cardiovascular replacements. The fabrication methods are divided into several sections—self-supporting grafts based on simple 3D bioprinting and bioprinting of bioinks on scaffolds made of decellularized or nanofibrous material.
Texture Perception and Chewing of Agar Gel by People with Different Sensitivity to Hardness
Authors: Smirnov Vasily , Khramova Daria , Chistiakova Elizaveta , Zueva Natalya , Vityazev Fedor , Velskaya Inga , Popov Sergey
Published: December 2024

Hardness is one of the dominant sensory characteristics of food. This study estimated the effect of sensitivity to hardness on the texture perception and chewing function using 2, 4, and 6% agar gels. Increasing the concentration of agar resulted in an increase in gel hardness and springiness, measured by texture profile analysis. Non-trained participants (n = 95) reported more subjective hardness and springiness during chewing gel samples as the agar concentration increased. Based on the relationship value of the instrumental and sensory data, all participants were divided into low-, medium-, and high-sensitivity groups (n = 25, 44, and 26). Low sensitivity to hardness was associated with low sensitivity to brittleness, springiness, chewiness, moisture, and swallowability. In all three groups, enhanced agar gel hardness increased the temporal chewing characteristics in a similar manner. However, in those with a high hardness sensitivity, the area amplitude of the masseter and temporalis muscles grew to a lesser extent than in those with a low or medium sensitivity. The activity of the suprahyoid muscles increased with the increasing agar gel hardness, regardless of sensitivity. All groups showed a similar salivation and bolus fragmentation while chewing gel. Thus, people’s sensitivity to hardness was associated with different perceptions of the gel’s textural properties and changes in masticatory muscle activity.
Quality Characteristics and In Vitro Digestibility of Starch Gel in White Noodles Prepared with Short-Chain Glucan Aggregates (SCGA)
Authors: Park Jae-Geun , Ye Sang-Jin , Oh Seon-Min , Shin Jae-Sung , Bae Ji-Eun , Choi Hyun-Wook , Baik Moo-Yeol
Published: December 2024

Short-chain glucan aggregates (SCGA), a type of resistant starch (RS) Ⅲ, is produced by debranching amylopectin with pullulanase and inducing self-assembly. Despite its low digestibility and high RS content, SCGA has not been applied to real food systems, especially noodles. The objective of this study was to determine the feasibility of low-digestible noodles using SCGA and to evaluate their quality characteristics and in vitro digestibility of starch gel. SCGA-noodles (SN) were prepared by substituting 0, 10, 20, and 25% of wheat flour with SCGA, and non-digestible maltodextrin (NMD) replaced 7% of the flour for comparison. Adding SCGA increased L- and b-values of the dough, resulting in a brighter appearance, while the NMD increased a-values. The substitution with SCGA weakened the gluten network, reducing dough and texture properties. Notably, cooked SN25 broke immediately in the tensile test, indicating substitution up to 20% is feasible in noodles. NMD7 formed sticky dough and showed extensive elongation without breaking. SN20 and SN25 significantly increased RS content and decreased the estimated glycemic index (eGI) compared to the control. However, NMD7 did not significantly reduce in vitro starch digestibility. In conclusion, this research confirmed the potential of SCGA as a low-digestible ingredient for noodles and other food applications.
Beyond Needles: Immunomodulatory Hydrogel-Guided Vaccine Delivery Systems
Authors: Rana Md Mohosin , Demirkaya Cigdem , De la Hoz Siegler Hector
Published: December 2024

Vaccines are critical for combating infectious diseases, saving millions of lives worldwide each year. Effective immunization requires precise vaccine delivery to ensure proper antigen transport and robust immune activation. Traditional vaccine delivery systems, however, face significant challenges, including low immunogenicity and undesirable inflammatory reactions, limiting their efficiency. Encapsulating or binding vaccines within biomaterials has emerged as a promising strategy to overcome these limitations. Among biomaterials, hydrogels have gained considerable attention for their biocompatibility, ability to interact with biological systems, and potential to modulate immune responses. Hydrogels offer a materials science-driven approach for targeted vaccine delivery, addressing the shortcomings of conventional methods while enhancing vaccine efficacy. This review examines the potential of hydrogel-based systems to improve immunogenicity and explores their dual role as immunomodulatory adjuvants. Innovative delivery methods, such as microneedles, patches, and inhalable systems, are discussed as minimally invasive alternatives to traditional administration routes. Additionally, this review addresses critical challenges, including safety, scalability, and regulatory considerations, offering insights into hydrogel-guided strategies for eliciting targeted immune responses and advancing global immunization efforts.
Contribution of Phosphorylation Modification to Stability and Antibacterial Activity of Egg White Protein Nanogels Loaded with Cinnamon Bark Essential Oil
Authors: Rao Sheng-Qi , Gao Xin-Ru , Liu Hui , Wang Zhi-Rong , Yang Zhen-Quan
Published: December 2024

This study evaluated the potential usage of phosphorylated egg white protein (P-EWP) nanogels fabricated via microwave-induced phosphorylation modification and gel process and further ultrasonic nanometrization as novel delivery systems for cinnamon bark essential oil (CBEO). Compared to EWP-CBEO nanogels without chemical phosphorylation, the obtained P-EWP-CBEO nanogels have shown smaller average hydrodynamic diameter (133.6 nm), relatively uniform size distribution (polydispersity index around 0.265), enhanced negative surface charge (−35.4 mV), and improved stability under the conditions of high temperature (up to 90 °C) and ionic strength (up to 200 mM NaCl). Moreover, P-EWP-CBEO nanogels, with hydrophobic interactions and disulfide bonds as the main intermolecular forces, exhibited a remarkable conformational change in microstructures. In addition, the results of the antibacterial experiments on Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes showed that the MIC values of P-EWP-CBEO nanogels were two times lower than those of EWP-CBEO nanogels and could completely inhibit the growth of pathogenic bacteria within 108 h. Hence, we have suggested that P-EWP-CBEO nanogels are successfully fabricated with improved physicochemical properties as novel potential natural preservatives in the food industry.
Genome-Wide Identification and Comprehensive Analysis of the PPO Gene Family in Glycine max and Glycine soja
Authors: Song Ziye , Wang Bo , Liu Jia , Liu Nianxi , Yi Zhigang , Li Zhi , Dong Zhimin , Zhang Chunbao , Dong Yingshan , Li Yuqiu
Published: December 2024

Background: Polyphenol oxidases (PPOs) form a multigene family that is widely distributed in plants, animals, and insects. To date, PPOs have been identified in plants such as Populus L. and Solanum tuberosum L., but studies on PPOs in soybean (Glycine max (L.) Merr.) and wild soybean (Glycine soja Sieb. and Zucc.) remain limited. Methods: To clarify the nature, structure, evolution, expression pattern, and interaction network of PPOs in these plants, we performed bioinformatics analysis and evaluated the expression patterns of PPOs in soybean and wild soybean throughout the growth period and under salt stress. Results: We identified 17 and 15 genes belonging to the PPO family. These genes were distributed across chromosomes 7 and 6 and could be divided into three groups. Most of these genes only contained one coding sequence (CDS), and their gene structure, conserved motifs, and 3D structures were very similar. Although there were a few intraspecies gene duplications, 75 gene replication pairs between soybean and wild soybean were detected. A Ka/Ks analysis showed that the PPOs in these plants were mainly subjected to purity selection. Moreover, the expression of the PPO genes varied greatly during different stages of the growth period and under salt stress, showing high temporal and spatial specificity. The protein interaction networks of these genes appeared to be quite distinct. Through the interaction analysis of the candidate gene GmPPO2 selected under salt stress, Glyma.07G059000, Glyma.10G279000, and Glyma.03G167900 were identified as the candidate genes regulating salt stress tolerance in soybean. Conclusions: These findings provide a foundation for further research on the evolution of soybean and wild soybean, as well as the functions of the PPO gene family.
Green Health—A New Open Access Journal
Authors: Lin Hualiang , Domingo Jose L.
Published: December 2024

In an era where global environmental and public health challenges intersect with emerging opportunities, the launch of the journal Green Health (ISSN 3042-5832) [...]
Associations of Body Mass Index and Percent Body Fat with Osteoporosis, Sarcopenia, and Osteosarcopenia: A Retrospective Study Based on Postmenopausal Women in China
Authors: Zhao Shengli , Hong Jiacong , Li Haonan , Zhang Xiaoyan , Wan Yong , Chen Bailing
Published: December 2024

Background/Objectives: Alterations in the body mass index (BMI) and percent body fat (PBF) have been considered to be related to aging-induced changes in bone and muscle. This study aimed to evaluate the associations of the BMI and PBF with osteoporosis, sarcopenia, and osteosarcopenia in postmenopausal women. Methods: A total of 342 participants who underwent musculoskeletal function assessments at the First Affiliated Hospital of Sun Yat-sen University between January 2015 and December 2022 were retrospectively screened. The diagnosis of osteoporosis was based on the WHO criteria, and sarcopenia was diagnosed according to the 2019 consensus of the Asian Working Group for Sarcopenia. Results: The BMI was positively correlated with the musculoskeletal function assessment parameters (bone mineral density, relative skeletal muscle index, and grip strength) and identified as an independent protective factor for sarcopenia alone (OR = 0.592, 95% CI: 0.455–0.769) or osteosarcopenia (OR = 0.411, 95% CI: 0.319–0.529), with a moderate diagnostic accuracy (area under the curve [AUC] = 0.682) for the former and a high diagnostic accuracy (AUC = 0.823) for the latter. However, the PBF was negatively correlated with the relative skeletal muscle index and identified as a risk factor for osteosarcopenia (OR = 1.404, 95% CI: 1.007–1.959), with a moderate diagnostic accuracy (AUC = 0.613). Conclusions: A higher BMI and lower PBF were associated with a lower prevalence of osteosarcopenia in postmenopausal women. Further research is required to elucidate the independent effects of the BMI and PBF on bone health.
Effect of Strength Training on Body Composition, Volumetrics and Strength in Female Breast Cancer Survivors
Authors: Pardo-Hernández Rocío , Fernández-Solana Jessica , González-Bernal Jerónimo J. , Romero-Pérez Ena Monserrat , Horta-Gim Mario Alberto , Riojas Pesqueira Luis Enrique , Muñoz-Alcaraz María Nieves , González-Santos Josefa , Santamaría-Peláez Mirian
Published: December 2024

Background/Aims: This cross-sectional study investigates body composition and strength in female breast cancer survivors, focusing on the effects of radical mastectomy and the presence of upper extremity lymphoedema. The main objective was to understand body composition, volumetry, and strength, as well as response to strength training in female breast cancer survivors. Methods: Twenty-three women (aged 42–74 years old) with radical mastectomy in the last five years were assessed by measuring body composition (weight, water percentage, fat, muscle, and lean mass), maximal strength, perimeters, and brachial volumes. Participants completed a 10-week strength training program of moderate intensity with 20 training sessions. No significant differences were found between the affected/healthy hemispheres in terms of composition, perimeters, and volumetrics. However, 11 women were found to have lymphoedema (47.8%). No statistically significant differences were found between hemibodies after the intervention, although improvements were obtained in pectoral strength and manual grip, as well as in muscle mass and lean mass [p = 0.002 each]. Cases with lymphoedema were reduced to 5 (21.73%). Conclusions: While strength training is shown to benefit body composition, strength, and the incidence of lymphoedema in mastectomized women, further scientific evidence is needed with larger controlled trials and follow-up studies to validate these findings, as well as the impact on the quality of life of these survivors.
Investigating the Effects of Optimized Mineral Fertilization on Plant Growth, Physiological Traits, Tuber Yield, and Biochemical Contents of Potato Crop
Authors: Chabani Hadjer , Tarchoun Neji , Amami Roua , Saadaoui Wassim , Mezghani Najla , Alexopoulos Alexios A. , Petropoulos Spyridon A.
Published: December 2024

Plants of two potato (Solanum tuberosum L.) varieties ‘Spunta’ (mid-early maturity) and ‘Kensa’ (mid-late maturity) were subjected to three nitrogen (N), phosphorus (P), and potassium (K) fertilization regimes, with T1 optimal rates (as recommended by the Tunisian Potato Technical Centre), T2 high rates (+25% of T1), and T3 low rates (−25% of T1). Plant growth, yield components, chlorophyll fluorescence (Fo, Fm, Fv/Fm), photosynthetic active radiation (PAR), real evapotranspiration (RET), and biochemical parameters in mature leaves and tubers (total soluble sugars (TSSs) and starch in both leaves and tubers) were evaluated. Our results showed a significant effect of fertilizer rates on plant growth, physiological, yield, and quality traits, as well as on biochemical contents of leaves and tubers, as well as on the variety. The application of high rates (T2) resulted in increased chlorophyll fluorescence (Fo) and high ratios of Fv/Fm, and it reduced Fm and photosynthetic active radiation (PAR). The highest yield per plant (615.4 g of tubers) and average number of tubers/plant (6.44) were observed in cv. ‘Spunta’ subjected to optimal fertilizer rate (T1), while more than 50% of tubers of this variety were classified as size C1 (>50 mm). On the other hand, high rates (T2) increased the yield per plant (436.74 g; approximately 9.3% compared to T1) and the number of tubers per plant (5.70) in cv. ‘Kensa’, with approximately 56% of tubers being classified in the C1 category. High rates also increased sucrose and starch content in tubers, regardless of the variety, without being significantly different from the other fertilization regimes. In conclusion, our results provide important information regarding the effect of fertilization practice on potato growth and yield parameters and the biochemical composition of leaves and tubers. Therefore, it could be suggested that the application of reduced NPK rates (−25% of optimal rates) in mid-early varieties (namely cv. ‘Spunta’) could reduce the production cost without compromising yield and quality components.
Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis
Authors: Ai Jiaqi , Wang Wuhong , Hu Tianhua , Hu Haijiao , Yan Yaqin , Wang Jinglei , Wang Yunzhu , Hu Na , Pang Hongtao , Bao Chonglai , Wei Qingzhen
Published: December 2024

MYB transcription factors (TFs) represent one of the largest gene families in plants, and previous studies have demonstrated their involvement in regulating anthocyanin synthesis. Eggplant is an important anthocyanin-rich solanaceae vegetable crop. In this study, a total of 219 MYB gene-family members were identified using the complete and high-quality eggplant genome, comprising 105 1R-MYBs, 107 R2R3-MYBs, 5 3R-MYBs, and 2 4R-MYBs. Using phylogenetic analysis, we divided them into 33 subfamilies. Members of the SmeMYB gene family are unevenly distributed on 12 chromosomes, but are mainly concentrated at the upper and lower ends of the chromosomes. In addition, the expression levels of R2R3-MYBs in differently colored eggplant tissues (peel, calyx, stem, flower, and leaf) were investigated with RNA-seq (RNA sequencing). A total of 13 differentially expressed R2R3-MYB transcription factors related to the synthesis of anthocyanins in different eggplant tissues were screened out. SmeMYB110, SmeMYB175, and SmeMYB182 were also found to play significant roles in this process. Furthermore, 10 MYB TFs were identified as potential genes regulating anthocyanin synthesis in different tissues. The quantitative real-time PCR (qRT-PCR) analysis results showed that SmeMYB175 was regarded as the most likely key transcription factor regulating anthocyanin synthesis in calyx. These results not only deepen our understanding of the MYB gene family in eggplant but also lay a solid foundation for further research on the regulation of SmeR2R3-MYBs in anthocyanin synthesis across diverse eggplant tissues.
Determining the Impact Bruising of Goji Berry Using a Pendulum Method
Authors: Jiang Yanwu , Chen Qingyu , Wei Naishuo
Published: December 2024

Lycium barbarum L. (goji), as an economic crop, has a high added value. However, the tender and fragile fruits are easily damaged during harvesting and transportation, leading to fruit bruising, which can cause rotting or black–brown spots after drying, seriously affecting the quality and price. In this study, two varieties of goji were used to determine and evaluate fruit bruising using a pendulum impact test, and the impact process was recorded using a high-speed camera and impact force sensor. This study discussed the energy changes during the impact process of fruits and conducted a correlation analysis of the impact energy, absorbed energy, restitution coefficient, impact force, and other indicators, analyzing the changes in each indicator with the falling height. The results showed that 0.2 m could be considered a critical height for damaging the fruit of goji. Furthermore, this study calculated the bruise susceptibility of the different varieties at different heights, which can be used for predicting bruising during the harvesting and collection of goji berries and ultimately for estimating the damage caused by mechanical harvesting.
A Method for Tomato Ripeness Recognition and Detection Based on an Improved YOLOv8 Model
Authors: Yang Zhanshuo , Li Yaxian , Han Qiyu , Wang Haoming , Li Chunjiang , Wu Zhandong
Published: December 2024

With the rapid development of agriculture, tomatoes, as an important economic crop, require accurate ripeness recognition technology to enable selective harvesting. Therefore, intelligent tomato ripeness recognition plays a crucial role in agricultural production. However, factors such as lighting conditions and occlusion lead to issues such as low detection accuracy, false detections, and missed detections. Thus, a deep learning algorithm for tomato ripeness detection based on an improved YOLOv8n is proposed in this study. First, the improved YOLOv8 model is used for tomato target detection and ripeness classification. The RCA-CBAM (Region and Color Attention Convolutional Block Attention Module) module is introduced into the YOLOv8 backbone network to enhance the model’s focus on key features. By incorporating attention mechanisms across three dimensions—color, channel, and spatial attention—the model’s ability to recognize changes in tomato color and spatial positioning is improved. Additionally, the BiFPN (Bidirectional Feature Pyramid Network) module is introduced to replace the traditional PANet connection, which achieves efficient feature fusion across different scales of tomato skin color, size, and surrounding environment and optimizes the expression ability of the feature map. Finally, an Inner-FocalerIoU loss function is designed and integrated to address the difficulty of ripeness classification caused by class imbalance in the samples. The results show that the improved YOLOv8+ model is capable of accurately recognizing the ripeness level of tomatoes, achieving relatively high values of 95.8% precision value and 91.7% accuracy on the test dataset. It is concluded that the new model has strong detection performance and real-time detection.
Accuracy Evaluation Method for Vector Data Based on Hexagonal Discrete Global Grid
Authors: Ma Yue , Li Guoqing , Zhao Long , Yao Xiaochuang
Published: December 2024

With the continuous advancement of technology for obtaining geographic spatial data, the accumulated volume of such data has been increasing, thus imposing higher demands on the storage, organization, and management of such data. As a new form of data management, the Discrete Global Grid System (DGGS) provides standardized descriptions and the exchange of geographic information on a global scale, enabling the efficient storage and application of large-scale global spatial data. Constituting a traditional type of GIS spatial data, vector data have advantages such as clear positions, implicit attributes, and suitability for map output. The representation of vector data in the global discrete grid network based on an equal-area projection, such as the hexagonal grid, fundamentally solves problems such as data redundancy, geometric deformation, and data discontinuity that arise when representing multiple vector data in a gridded format. This paper proposes different gridding methods for various types of vector data, and a quantifiable accuracy evaluation system is established from the perspectives of geographical deviation, geometric features, and topological relationships, to evaluate the accuracy of the gridded vector data, covering all types of gridded vector data based on the hexagonal grid. The evaluation method is generally applicable to all hexagonal-grid-based gridded vector data, and can be generalized based on application scenarios, for evaluating the usability of hexagonal grid vector data.
Genome-Wide Identification and Expression Analysis of HSP70 Gene Family Under High-Temperature Stress in Lettuce (Lactuca sativa L.)
Authors: Wang Qian , Sun Wenjing , Duan Yipei , Xu Yikun , Wang Huiyu , Hao Jinghong , Han Yingyan , Liu Chaojie
Published: December 2024

The heat shock protein 70 (HSP70) family plays an important role in the growth and development of lettuce and in the defense response to high-temperature stress; however, its bioinformatics analysis in lettuce has been extremely limited. Genome-wide bioinformatics analysis methods such as chromosome location, phylogenetic relationships, gene structure, collinearity analysis, and promoter analysis were performed in the LsHSP70 gene family, and the expression patterns in response to high-temperature stress were analyzed. The mechanism of LsHSP70-19 in heat resistance in lettuce was studied by virus-induced gene silencing (VIGS) and transient overexpression techniques. The results showed that a total of 37 LsHSP70 genes were identified by the Hidden Markov Model (HMM) and Protein Family Database (Pfam). These 37 LsHSP70 genes were classified into groups A, B, C, and D by phylogenetic relationships. They were mainly localized on seven chromosomes except for chromosome 3; gene structure analysis showed that LsHSP70 contained 1–9 exons, and the protein structure domains of genes in the same group were highly conserved. The covariance analysis showed that nine pairs of LsHSP70 genes existed between LsHSP70 members, and lettuce LsHSP70 and sunflower HaHSP70 had been more conserved in the evolutionary process. The promoter analysis showed that there were a large number of cis-acting elements related to phytohormones, growth, development, stress, and light response in LsHSP70. In addition, the results of the expression pattern analysis for all LsHSP70 genes under high-temperature stress showed that 28 out of 37 LsHSP70 genes were able to respond to heat stress, and only LsHSP70-8, LsHSP70-14, LsHSP70-19, LsHSP70-23, and LsHSP70-24 were able to respond rapidly to heat stress (2 h). The expression of LsHSP70-19 was higher at different periods under high-temperature stress; the overexpression of LsHSP70-19, the plant fresh weight, and the root weight were better than the control (CK); and the heat resistance was better. These results suggest that LsHSP70-19 may play an important role under high-temperature stress in lettuce.
microRNA as an Important Mediator in the Regulation of Male Gallus gallus domesticus Reproduction: Current State of the Problem
Authors: Pozovnikova Marina , Ivershina Anastasiya , Stanishevskaya Olga , Silyukova Yuliya
Published: December 2024

During all periods of male ontogenesis, physiological processes responsible for the correct functioning of reproductive organs and spermatogenesis are under the influence of various factors (neuro-humoral, genetic, and paratypical). Recently, the attention of researchers has increasingly turned to the study of epigenetic factors. In scientific publications, one can increasingly find references to the direct role of microRNAs, small non-coding RNAs involved in post-transcriptional regulation of gene expression, in the processes of development and functioning of reproductive organs. Although the role of microRNAs in the reproduction of mammals, including humans, has been intensively studied, this area of knowledge in birds remains under-researched and limited to single experiments. This is likely due to the unique features of embryogenesis and the structure of the avian reproductive system. This review summarizes the current state of knowledge on the role of microRNAs in avian reproduction. Insight into the molecular basis of spermatogenesis in Gallus gallus domesticus is provided. Data on the functions and mechanisms by which microRNAs influence the processes of growth, development, and formation of rooster germ cells that determine the necessary morphofunctional qualitative characteristics of mature spermatozoa are summarized. Particular attention is paid to miRNA biogenesis as an important step affecting the success of spermatogenesis, as well as the role of miRNAs in avian sex differentiation during early embryogenesis. The modern literature sources systematized in this review, revealing the questions about the role of miRNAs in the reproductive function of birds, create a theoretical basis and define new perspectives and directions for further research in this field.
Cochlear Mechanics Are Preserved After Inner Ear Delivery of Gold Nanoparticles
Authors: Pan Dorothy W. , Kim Jinkyung , Quiñones Patricia M. , Ricci Anthony J. , Applegate Brian E. , Oghalai John S.
Published: December 2024

Novel therapeutic delivery systems and delivery methods to the inner ear are necessary to treat hearing loss and inner ear disorders. However, numerous barriers exist to therapeutic delivery into the bone-encased and immune-privileged environment of the inner ear and cochlea, which makes treating inner ear disorders challenging. Nanoparticles (NPs) are a type of therapeutic delivery system that can be engineered for multiple purposes, and posterior semicircular canal (PSCC) infusion is a method to directly deposit them into the cochlea. We sought to assess PSCC infusion of gold NPs into the cochlea, including the NPs’ distribution and effect on cochlear mechanics. We performed optical coherence tomography (OCT) imaging to monitor PSCC infusion of gold NPs into the cochlear chambers. OCT imaging demonstrated that the infusion specifically targeted the perilymphatic spaces within the cochlea. We assessed cochlear mechanics by using OCT vibrometry to measure sound-evoked movements of the basilar membrane. We found no changes in cochlear mechanics between measurements at baseline, after the PSCC canalostomy, immediately after the infusion, and 1 h after the infusion of gold NPs (p > 0.05, paired t-test). These findings validate the PSCC infusion approach for perfusing the cochlear perilymphatic space with a nanoparticle delivery system. Thus, PSCC infusion of nanoparticles is a feasible therapeutic delivery technique for treating inner ear disorders while preserving residual cochlear function.
PTHrP Promotes RBP4 Expression Under the Control of PPARγ in the Kidney
Authors: Nieto-Bona María Paz , Carrasco Almudena G. , Medina-Gomez Gema , Bosch Ricardo J. , Izquierdo-Lahuerta Adriana
Published: December 2024

Parathyroid hormone-related protein (PTHrP) and retinol-binding protein 4 (RBP4) have been associated with a worse prognosis of kidney disease. Recently, the direct interconnection between PTHrP and the peroxisome proliferator-activated receptor gamma (PPARγ), a nuclear receptor whose activation is nephroprotective, has been discovered. The aim of this study was to analyze the relationship between PTHrP, PPARγ, and RBP4. For this purpose, we analyzed the levels of these proteins, which were studied in the kidneys of five experimental groups of mice at 6 weeks of age: controls, diabetics, insulin-treated diabetics, transgenic mice overexpressing PTHrP at the renal level, and the latter mice that were also induced with diabetes. In addition, we also analyzed the expression levels of these molecules in two mouse podocyte cell lines, controls and PPARγKO, subjected to a lipotoxic insult by palmitic acid. We found that RBP4 and PTHrP are increased in the kidney in pathological conditions and that insulin and PPARγ act regulating PTHrP and RBP4 expression, suggesting that the regulation of this system is critical for the maintenance of renal homeostasis and how it becomes imbalanced in different pathophysiological conditions.
Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways
Authors: Wu Chenxi , Xu Xinru , Shi Yueyue , Li Fenfen , Zhang Xiaoxi , Huang Yan , Xia Daozong
Published: December 2024

Neutrophil extracellular traps (NETs) formation is a key process in inflammatory diseases like gout, but the underlying molecular mechanisms remain incompletely understood. This study aimed to establish a model to examine the formation of NETs induced by monosodium urate (MSU) and phorbol 12-myristate 13-acetate (PMA) and to elucidate their molecular pathways. Laser confocal microscopy was used to visualize NET formation, while flow cytometry was employed to detect reactive oxygen species (ROS) production. The microstructure of neutrophils was observed by transmission electron microscopy, and the expression of key proteins was determined by Western blotting. Additionally, the effect of various inhibitors targeting the MAPK signaling pathway on NET formation was evaluated. They include the Ras inhibitor Salirasib, Raf inhibitor Vemurafenib, ERK inhibitor PD98059, and p38 MAPK inhibitor SB203580, as well as NADPH oxidase inhibitor DPI and neutrophil elastase inhibitor Alvelestat. The results showed that MSU and PMA triggered significant NET formation, which was accompanied by increased ROS levels, lactate dehydrogenase release, dsDNA, and IL-8. Notably, selective MAPK pathway inhibitors and DPI and Alvelestat, except for SB203580, effectively down-regulated these indicators. These data indicated that the activation of a signaling pathway involving Ras-Raf-ERK, which is dependent on ROS, is crucial for the induction of NET formation by MSU and PMA. Given the involvement of NETs in multiple pathologies, our findings could potentially serve as molecular targets for the intervention and treatment of crystal-related diseases, especially for gout.
Characterization of Mesenchymal and Neural Stem Cells Response to Bipolar Microsecond Electric Pulses Stimulation
Authors: Innamorati Giorgia , Sanchez-Petidier Marina , Bergafora Giulia , Codazzi Camilla , Palma Valentina , Camera Francesca , Merla Caterina , André Franck M. , Pedraza Maria , Moreno Manzano Victoria , Caramazza Laura , Colella Micol , Marracino Paolo , Balucani Marco , Apollonio Francesca , Liberti Micaela , Consales Claudia
Published: December 2024

In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that stem cell fate can be controlled by applying continuous electric field stimulation. This study aims to characterize the effect of a specific microsecond electric pulse stimulation (bipolar pulses of 100 µs + 100 µs, delivered for 30 min at an intensity of 250 V/cm) to induce an increase in cell proliferation on mesenchymal stem cells (MSCs) and induced neural stem cells (iNSCs). The effect was evaluated in terms of (i) cell counting, (ii) cell cycle, (iii) gene expression, and (iv) apoptosis. The results show that 24 h after the stimulation, cell proliferation, cell cycle, and apoptosis are not affected, but variation in the expression of specific genes involved in these processes is observed. These results led us to investigate cell proliferation until 72 h from the stimulation, observing an increase in the iNSCs number at this time point. The main outcome of this study is that the microsecond electric pulses can modulate stem cell proliferation.
Polystyrene Microplastics Induce Photosynthetic Impairment in Navicula sp. at Physiological and Transcriptomic Levels
Authors: Li Xi , Wang Zunyan , Chen Yiyong , Li Qi
Published: December 2024

The rising concentration of microplastics (MPs) in aquatic environments poses increasing ecological risks, yet their impacts on biological communities remain largely unrevealed. This study investigated how aminopolystyrene microplastics (PS-NH2) affect physiology and gene expression using the freshwater alga Navicula sp. as the test species. After exposing Navicula sp. to high PS-NH2 concentrations for 24 h, growth was inhibited, with the most significant effect seen after 48 h. Increasing PS-NH2 concentrations reduced chlorophyll content, maximum photochemical quantum yield (Fv/Fm), and the photochemical quenching coefficient (Qp), while the non-photochemical quenching coefficient (NPQ) increased, indicating a substantial impact on photosynthesis. PS-NH2 exposure, damaged cell membrane microstructures, activated antioxidant enzymes, and significantly increased malondialdehyde (MDA), glutathione peroxidase (GPX), and superoxide dismutase (SOD) activities. Transcriptomic analysis revealed that PS-NH2 also affected the gene expression of Navicula sp. The differentially expressed genes (DEGs) are mainly related to porphyrin and chlorophyll metabolism, carbon fixation in photosynthesis, endocytosis, and glycolysis/gluconeogenesis. Protein–protein interaction (PPI) analysis revealed significant interactions among DEGs, particularly within photosystem II. These findings shed insights into the toxic mechanisms and environmental implications of microplastic interactions with phytoplankton, deepening our understanding of the potential adverse effects of microplastics in aquatic ecosystems.
Cannabinoids: Role in Neurological Diseases and Psychiatric Disorders
Authors: Kumar Ujendra
Published: December 2024

An impact of legalization and decriminalization of marijuana is the gradual increase in the use of cannabis for recreational purposes, which poses a potential threat to society and healthcare systems worldwide. However, the discovery of receptor subtypes, endogenous endocannabinoids, and enzymes involved in synthesis and degradation, as well as pharmacological characterization of receptors, has led to exploration of the use of cannabis in multiple peripheral and central pathological conditions. The role of cannabis in the modulation of crucial events involving perturbed physiological functions and disease progression, including apoptosis, inflammation, oxidative stress, perturbed mitochondrial function, and the impaired immune system, indicates medicinal values. These events are involved in most neurological diseases and prompt the gradual progression of the disease. At present, several synthetic agonists and antagonists, in addition to more than 70 phytocannabinoids, are available with distinct efficacy as a therapeutic alternative in different pathological conditions. The present review aims to describe the use of cannabis in neurological diseases and psychiatric disorders.
Nitric Oxide Pre-Treatment Advances Bulblet Dormancy Release by Mediating Metabolic Changes in Lilium
Authors: Yang Chenglong , Xu Xiaoping , Ali Muhammad Moaaz , He Xing , Guo Wenjie , Chen Faxing , Fang Shaozhong
Published: December 2024

The lily is a globally popular cut flower, and managing dormancy in lily bulblets is essential for continuous, year-round production. While nitric oxide (NO) has been shown to influence seed dormancy and germination, its role in dormancy release in lilies was previously unconfirmed. In this study, we investigated the effects of NO on dormancy release in lily bulblets using SNP and c-PTIO. Results showed that SNP treatment promoted dormancy release, while c-PTIO inhibited it. Measurement of endogenous NO levels in the bulbs, along with enzyme activities of NOS-like and NR and gene expression levels of LoNOS-IP and LoNR, confirmed that NO plays a role in promoting dormancy release in lilies. To further elucidate the physiological mechanisms involved, we analyzed H2O2 levels, antioxidant enzyme activities, endogenous hormone levels, and carbohydrate metabolism in the bulbs. Findings demonstrated that NO facilitated dormancy release by increasing H2O2, gibberellins (GAs), indole-3-acetic acid (IAA), zeatin riboside (ZR), reducing sugars, and by accelerating the metabolism of abscisic acid (ABA) and starch. This study provides a foundation for deeper investigation into the mechanisms underlying dormancy release in lily bulbs.
Recent Advances and Future Prospects of Lithium Recovery from Low-Grade Lithium Resources: A Review
Authors: Gu Jihan , Liang Binjun , Luo Xianping , Zhang Xin , Yuan Weiquan , Xiao Bin , Tang Xuekun
Published: December 2024

The growing demand for lithium, driven by the widespread adoption of electric vehicles and renewable energy storage systems, has sparked interest in developing low-grade lithium resources. This comprehensive review explores the types, distribution, extraction technologies, challenges, and future prospects of low-grade lithium resources. This article focuses on low-grade lithium sources such as clays, brines, coal, and coal by-products, and analyzes the principles, advantages, and limitations of key extraction techniques, including acid-alkaline leaching, bioleaching, adsorption, and membrane separation. Furthermore, this review discusses the technical, economic, and environmental sustainability challenges associated with developing low-grade lithium resources and proposes corresponding strategies. Future research should focus on improving the selectivity and efficiency of extraction and processing technologies, optimizing separation processes, and developing green and cost-effective extraction methods. Establishing supportive policy frameworks, strengthening international cooperation, and knowledge sharing are crucial for promoting the sustainable development of low-grade lithium resources. This review provides stakeholders with comprehensive insights and recommendations for addressing the growing lithium demand and achieving the Sustainable Development Goals.
A Novel Fluorescent Chemosensor Based on Rhodamine Schiff Base: Synthesis, Photophysical, Computational and Bioimaging Application in Live Cells
Authors: Aduroja Oyedoyin , Shaw Roosevelt , Uota Sisay , Abiye Isaac , Wachira James , Abebe Fasil
Published: December 2024

A novel rhodamine-6G derivative RdN was synthesized by condensing rhodamine glyoxal and 3-hydroxy-2-naphthoic hydrazide using a microwave irradiation-assisted reaction. Colorimetric and photophysical studies have demonstrated that the molecule produced can selectively sense Pb2+ and Cu2+ ions in a solution of CH3CN/H2O (9:1, v/v). The spirolactam ring of RdN opens upon complexation with the cations, forming a highly fluorescent complex and a visible color change in the solution. The compound RdN was further studied with the help of computational methods such as the Density Functional Theory (DFT) method and time-dependent density theory (TD-DFT) calculations to study the binding interactions and properties of the molecule. DFT calculations and job plot data supported the 2:1 complex formation between RdN and Pb2+/Cu2+. The limit of detection for Pb2+ was determined to be 0.112 µM and 0.130 µM for Cu2+. The probe RdN was applied to the image of Pb2+ and Cu2+ ions in living cells and is safe for biomedical applications. It is used to monitor Pb2+ in environmental water samples.
Microbiome of Hyalomma dromedarii (Ixodida: Ixodidae) Ticks: Variation in Community Structure with Regard to Sex and Host Habitat
Authors: Perveen Nighat , Cabezas-Cruz Alejandro , Iliashevich Daniil , Abuin-Denis Lianet , Sparagano Olivier Andre , Willingham Arve Lee
Published: December 2024

Hyalomma dromedarii ticks are the main hematophagous ectoparasites of camels, harboring a variety of microbes that can affect tick vector competence and pathogen transmission. To better understand the tick microbiome influenced by sex and host habitat, we analyzed the bacterial community of H. dromedarii male and female ticks collected from camel farms, livestock markets, and slaughterhouses, representing the range of major habitats in the UAE, by sequencing the 16S rRNA gene. Tick samples were collected during 2022 and 2023. A total of 40 ticks (male (15), female (15), and nymph (10)) were selected from tick samples collected from camels and processed for genomic DNA and next-generation sequencing using the Illumina MiSeq platform. We obtained 151,168 read counts, and these formed 237 operational taxonomic units representing 11 phyla, 22 classes, 77 families, and 164 genera. The phyla Actinomycetota, Bacillota, Bacteroidota, Pseudomonadota, and Fusobacteriota were the most abundant. The bacterial genus Corynebacterium dominated the microbiomes of farm-collected female H. dromedarii ticks, while Proteus dominated the microbiomes of farm-collected male H. dromedarii ticks. In comparison, the microbiomes of H. dromedarii ticks collected from slaughterhouse samples were dominated by genus Francisella in both males and females. Our results confirm that the bacterial microbiomes of H. dromedarii ticks vary by sex and habitat settings. Furthermore, recent findings could deliver insight into the differences in the ability of camel ticks to acquire, maintain, and transmit pathogens in various habitats that may impact the tick vector competence of medically and agriculturally important species in the Middle East and North Africa (MENA) region and Asia.