bluuebunny/crossref_metadata_2025 huggingface dataset:
Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies
Authors: Magistrelli Giovanni , Pontini Guillemette , Poitevin Yves , Malinge Pauline , Bourguignon Jérémie , Gauye Florence , Fleury Elise , Plèche Nicolas , Galissaires Lydia , Fischer Nicolas
Published: August 2018

Bispecific antibodies (bsAbs) are often composed of several polypeptide chains that have to be expressed adequately to enable optimal assembly and yield of the bsAb. κλ bodies are a bispecific format with a native IgG structure, composed of two different light chains that pair with a common heavy chain. Introduction of non-optimal codons into the sequence of a particular polypeptide is an effective strategy for down modulating its expression. Here we applied this strategy but restricted the modification of the codon content to the constant domain of one light chain. This approach facilitates parallel optimization of several bsAbs by using the same modified constant domains. Partial sequence de-optimization reduced expression of the targeted polypeptide. Stable cell pools could be isolated displaying increased bispecific antibody titers as well as changes in the abundance of undesired by-products that require elimination during downstream processing. Thus, modulating the relative expression of polypeptides can have a significant impact on bsAb titer and product related impurities; which are important factors for large scale manufacturing for clinical supply.
Prototype Design and Performance Tests of Beijing Astronaut Robot
Authors: Sun Zeyuan , Li Hui , Jiang Zhihong , Song Zhenzi , Mo Yang , Ceccarelli Marco
Published: August 2018

This paper proposes a novel chameleon-like astronaut robot that is designed to assist, or even substitute, a human astronauts in a space station to complete dangerous and prolonged work, such as maintenance of solar panels, and so on. The robot can move outside the space station freely via the hundreds of aluminum handrails, which are provided to help astronauts move. The robot weighs 30 kilograms, and consists of a torso, three identical 4-degree of freedom (DOF) arms, three end effectors, and three monocular vision system on each end effector. Via multi-arm associated motion, the robot can realize three kinds of motion modes: walking, rolling, and sliding. Numerous experiments have been conducted in a simulation environment and a ground verification platform. Experimental results reveal that this robot has excellent motion performance.
Health Monitoring for Balancing Tail Ropes of a Hoisting System Using a Convolutional Neural Network
Authors: Zhou Ping , Zhou Gongbo , Zhu Zhencai , Tang Chaoquan , He Zhenzhi , Li Wei , Jiang Fan
Published: August 2018

With the arrival of the big data era, it has become possible to apply deep learning to the health monitoring of mine production. In this paper, a convolutional neural network (CNN)-based method is proposed to monitor the health condition of the balancing tail ropes (BTRs) of the hoisting system, in which the feature of the BTR image is adaptively extracted using a CNN. This method can automatically detect various BTR faults in real-time, including disproportional spacing, twisted rope, broken strand and broken rope faults. Firstly, a CNN structure is proposed, and regularization technology is adopted to prevent overfitting. Then, a method of image dataset description and establishment that can cover the entire feature space of overhanging BTRs is put forward. Finally, the CNN and two traditional data mining algorithms, namely, k-nearest neighbor (KNN) and an artificial neural network with back propagation (ANN-BP), are adopted to train and test the established dataset, and the influence of hyperparameters on the network diagnostic accuracy is investigated experimentally. The experimental results showed that the CNN could effectively avoid complex steps such as manual feature extraction, that the learning rate and batch-size strongly affected the accuracy and training efficiency, and that the fault diagnosis accuracy of CNN was 100%, which was higher than that of KNN and ANN-BP. Therefore, the proposed CNN with high accuracy, real-time functioning and generalization performance is suitable for application in the health monitoring of hoisting system BTRs.
Partial Block Scheme and Adaptive Update Model for Kernelized Correlation Filters-Based Object Tracking
Authors: Jeong Soowoong , Paik Joonki
Published: August 2018

In visual object tracking, the dynamic environment is a challenging issue. Partial occlusion and scale variation are typical challenging problems. We present a correlation-based object tracking based on the discriminative model. To attenuate the influence by partial occlusion, partial sub-blocks are constructed from the original block, and each of them operates independently. The scale space is employed to deal with scale variation using a feature pyramid. We also present an adaptive update model with a weighting function to calculate the frame-adaptive learning rate. Theoretical analysis and experimental results demonstrate that the proposed method can robustly track drastic deformed objects. The sparse update reduces the computational cost for real-time tracking. Although the partial block scheme generation increases the computational cost, we present a novel sparse update approach to reduce the computational cost drastically for real-time tracking. The experiments were performed on a variety of sequences, and the proposed method exhibited better performance compared with the state-of-the-art trackers.
Advances in Micro-Bioreactor Design for Organ Cell Studies
Authors: Mandenius Carl-Fredrik
Published: August 2018

n/a
Targeting the Microenvironment in High Grade Serous Ovarian Cancer
Authors: Nwani Nkechiyere G. , Sima Livia E. , Nieves-Neira Wilberto , Matei Daniela
Published: August 2018

Cancer–stroma interactions play a key role in cancer progression and response to standard chemotherapy. Here, we provide a summary of the mechanisms by which the major cellular components of the ovarian cancer (OC) tumor microenvironment (TME) including cancer-associated fibroblasts (CAFs), myeloid, immune, endothelial, and mesothelial cells potentiate cancer progression. High-grade serous ovarian cancer (HGSOC) is characterized by a pro-inflammatory and angiogenic signature. This profile is correlated with clinical outcomes and can be a target for therapy. Accumulation of malignant ascites in the peritoneal cavity allows for secreted factors to fuel paracrine and autocrine circuits that augment cancer cell proliferation and invasiveness. Adhesion of cancer cells to the mesothelial matrix promotes peritoneal tumor dissemination and represents another attractive target to prevent metastasis. The immunosuppressed tumor milieu of HGSOC is permissive for tumor growth and can be modulated therapeutically. Results of emerging preclinical and clinical trials testing TME-modulating therapeutics for the treatment of OC are highlighted.
NiFeOx as a Bifunctional Electrocatalyst for Oxygen Reduction (OR) and Evolution (OE) Reaction in Alkaline Media
Authors: Paulraj Alagar Raj , Kiros Yohannes , Göthelid Mats , Johansson Malin B.
Published: August 2018

This article reports the two-step synthesis of NiFeOx nanomaterials and their characterization and bifunctional electrocatalytic activity measurements in alkaline electrolyte for metal-air batteries. The samples were mostly in layered double hydroxide at the initial temperature, but upon heat treatment, they were converted to NiFe2O4 phases. The electrochemical behaviour of the different samples was studied by linear sweep voltammetry and cyclic voltammetry on the glassy carbon electrode. The OER catalyst activity was observed for low mass loadings (0.125 mg cm−2), whereas high catalyst loading exhibited the best performance on the ORR side. The sample heat-treated at 250 °C delivered the highest bi-functional oxygen evolution and reduction reaction activity (OER/ORR) thanks to its thin-holey nanosheet-like structure with higher nickel oxidation state at 250 °C. This work further helps to develop low-cost electrocatalyst development for metal-air batteries.
Validation of the Names of Four Weevil Species Described by Caldara & Košťál, Description of Four New Species of the Afrotropical Weevil Genus Afroryzophilus (Coleoptera, Curculionidae); Diversity 2018, 10, 37
Authors: Caldara Roberto , Košťál Michael
Published: August 2018

Four new species of the erirhinine genus Afroryzophilus Lyal, 1990 from Africa are described, A. centrafricanus sp. n., A. congoanus sp. n., A. kuscheli sp. n. and A. somalicus sp. n., with bibliographic reference to fuller descriptions and illustrations in the recent paper by Caldara & Košťál (2018) published in the journal Diversity 10 (2), 37, in which the names were not made available under the rules of the International Code of Zoological Nomenclature dealing with electronic publication.
Work Availability and Exergy Analysis
Authors: Ahmadi Pouria , Rezaie Behnaz
Published: August 2018

n/a
Entropy Applications in Environmental and Water Engineering
Authors: Cui Huijuan , Sivakumar Bellie , Singh Vijay
Published: August 2018

n/a
A DC Short-Circuit Fault Ride Through Strategy of MMC-HVDC Based on the Cascaded Star Converter
Authors: Wang Yingjie , Yang Bo , Zuo Huifang , Liu Haiyuan , Yan Haohao
Published: August 2018

A modular multilevel converter based high voltage direct current (MMC-HVDC) with DC fault self-clearing is adopted to deal with the DC short-circuit fault. However, the constant power load characteristic of the sub-modules causes capacitor voltages to diverge and the converter to go out of hot standby. To address this problem, a novel DC short-circuit fault ride through strategy is proposed. According to the polarities of grid voltages, the working or blockage of the upper and lower bridge arms is chosen according to six sections to obtain a cascaded star converter. The capacitor voltages of MMC sub-modules are maintained and balanced through the control similar to the cascaded star converter. Moreover, in order not to change zero crossing, a cluster balancing control method by scaling the amplitudes of the modulated waves is proposed to balance the capacitor voltages between phase clusters. The strategy also achieves the DC Bus line-to-line equipotential and no fault current generated. With the switches of two modes (normal operation and fault ride through operation) after the fault is cleared, the power transfer of MMC-HVDC can be recovered quickly. Finally, the effectiveness of the proposed fault ride through strategy is demonstrated on the 21-level MMC-HVDC simulation model in PSCAD/EMTDC.
Empirical Comparison of Neural Network and Auto-Regressive Models in Short-Term Load Forecasting
Authors: López Miguel , Sans Carlos , Valero Sergio , Senabre Carolina
Published: August 2018

Artificial Intelligence (AI) has been widely used in Short-Term Load Forecasting (STLF) in the last 20 years and it has partly displaced older time-series and statistical methods to a second row. However, the STLF problem is very particular and specific to each case and, while there are many papers about AI applications, there is little research determining which features of an STLF system is better suited for a specific data set. In many occasions both classical and modern methods coexist, providing combined forecasts that outperform the individual ones. This paper presents a thorough empirical comparison between Neural Networks (NN) and Autoregressive (AR) models as forecasting engines. The objective of this paper is to determine the circumstances under which each model shows a better performance. It analyzes one of the models currently in use at the National Transport System Operator in Spain, Red Eléctrica de España (REE), which combines both techniques. The parameters that are tested are the availability of historical data, the treatment of exogenous variables, the training frequency and the configuration of the model. The performance of each model is measured as RMSE over a one-year period and analyzed under several factors like special days or extreme temperatures. The AR model has 0.13% lower error than the NN under ideal conditions. However, the NN model performs more accurately under certain stress situations.
Numerical Investigation of the Effects of Steam Mole Fraction and the Inlet Velocity of Reforming Reactants on an Industrial-Scale Steam Methane Reformer
Authors: Yeh Chun-Lang
Published: August 2018

Steam methane reforming (SMR) is the most common commercial method of industrial hydrogen production. Control of the catalyst tube temperature is a fundamental demand of the reformer design because the tube temperature must be maintained within a range that the catalysts have high activity and the tube has minor damage. In this paper, the transport and chemical reaction in an industrial-scale steam methane reformer are simulated using computational fluid dynamics (CFD). Two factors influencing the reformer temperature, hydrogen yield and stress distribution are discussed: (1) the mole fraction of steam (YH2O) and (2) the inlet velocity of the reforming reactants. The purpose of this paper is to get a better understanding of the flow and thermal development in a reformer and thus, to make it possible to improve the performance and lifetime of a steam reformer. It is found that the lowest temperature at the reforming tube surface occurs when YH2O is 0.5. Hydrogen yield has the highest value when YH2O is 0.5. The wall shear stress at the reforming tube surface is higher at a higher YH2O. The surface temperature of a reforming tube increases with the inlet velocity of the reforming reactants. Finally, the wall shear stress at the reforming tube surface increases with the inlet velocity of the reforming reactants.
A 120 W Class-E Power Module with an Adaptive Power Combiner for a 6.78 MHz Wireless Power Transfer System
Authors: Choi Ui-Gyu , Yang Jong-Ryul
Published: August 2018

In this article, a highly efficient power module is presented with two class-E power amplifiers and an adaptive power combiner for transmitting output powers >100 W at 6.78 MHz in a wireless power transfer system. The losses caused by the combiners and interstage matching circuits or mismatching between the amplifier, and the combiners can significantly reduce the overall efficiency of the power module. To achieve an efficient combination of the output amplifier signals, the adaptive power combiner is proposed based on the consideration of the optimum load impedance characteristics of the power amplifiers. The input impedance of the combiner is designed using series capacitors and resistors between the two input ports of the combiner and the two output signals of the class-E amplifiers at the optimum load condition. The output performances of the proposed module can decrease based on the component mismatch between the two power amplifiers. The proposed power module was implemented on an FR4 PCB, with a 15 mm metal heat sink, and demonstrated an output power of 123.3 W, a power-added efficiency of 85.7%, and a power gain of 25.6 dB at 6.78 MHz. The second harmonic suppression of the module was 37 dBc.
A New Gob-Side Entry Layout Method for Two-Entry Longwall Systems
Authors: Wu Rui , He Qingyuan , Oh Joung , Li Zecheng , Zhang Chengguo
Published: August 2018

The gob-side entry layout is popular at two-entry longwall mine sites in China for the benefit of improving the coal recovery rate. Currently, two methods have been widely used to develop gob-side entries, including gob-side entry retaining and gob-side entry driving. Gob-side entry retaining maximizes the recovery rate by pillarless mining but increases the difficulty in gob-side entry support. Also, this method has limited applications in hard roof conditions. The gob-side entry driving mine site uses the rib pillar to separate the gob entry and the gob area of the previous panel, which leads to additional coal losses. The waste is more intolerable in large-cutting-height panels and longwall top coal caving panels as the Chinese government limits the minimum recovery rate of longwall panels using these mining methods. In this paper, a new gob-side entry layout method, termed gob-side pre-backfill driving, is established to overcome the shortcomings of the existing methods. The new method eliminates rib pillar losses and enhances gob-side entry stability. The feasibility of gob-side pre-backfill driving is studied by numerical modelling and a field trial at Changcun Mine in China. The results indicate that gob-side pre-backfill driving is an alternative for gob-side entry development. This method is practical and also has the potential to bring significant economic benefits to the mining industry.
Italian Experience on Electrical Storage Ageing for Primary Frequency Regulation
Authors: Benato Roberto , Dambone Sessa Sebastian , Musio Maura , Palone Francesco , Polito Rosario Maria
Published: August 2018

The paper describes the results of different types of ageing tests performed by Terna (the Italian Transmission System Operator) applied to several electrochemical technologies, namely lithium-based and sodium-nickel chloride-based technologies. In particular, the tested lithium-based technologies exploit a graphite-based anode and the following cathode electrochemistries: lithium iron phosphate, lithium nickel cobalt aluminium, lithium nickel cobalt manganese, and lithium titanate. These tests have been performed in the storage labs located in Sardinia (Codrongianos) and Sicily (Ciminna). The aim of the storage labs is intended to give the electrical grid ancillary services, for example, primary frequency regulation, secondary frequency regulation, voltage regulation, synthetic rotational inertia provision, and many more. For the primary frequency regulation service, the ageing of the batteries is difficult to foresee as the ageing tests are not standardized. The authors proposed some novel cycle types, which showed that, in several cases, the frequency regulation cycle ages the batteries much more than the standard cycle. The standard cycle definition has been adopted in the paper to identify a battery cycle test that was carried out to uniformly compare and rank the different technologies. Moreover, sodium-nickel chloride batteries are unaffected by the types of cycle and have a negligible ageing. In addition, lithium manganese oxide and lithium titanate batteries show very good behaviour with a slight degradation of the dischargeable energy, irrespectively of the type of cycle. Inversely, lithium nickel cobalt aluminium technology shows a considerable ageing and a strong dependence on the cycle types. Even if the theoretical explanations of such aging behaviours need time to be understood and expounded, the authors are convinced that the scientific community should become aware of these experimental results.
Partial Redesign of an Accelerator Driven System Target for Optimizing the Heat Removal and Minimizing the Pressure Drops
Authors: Lomonaco Guglielmo , Alessandroni Giacomo , Borreani Walter
Published: August 2018

Accelerator Driven Systems (ADS) seem to be a good solution for safe nuclear waste transmutation. One of the most important challenges for this kind of machine is the target design, particularly for what concerning the target cooling system. In order to optimize this component a CFD-based approach has been chosen. After the definition of a reference design (Be target cooled by He), some parameters have been varied in order to optimize the thermal-fluid-dynamic features. The final optimized target design has an increased security margin for what regarding Be melting and reduces the maximum coolant velocity (and consequently even more the pressure drops).
Formulation of Broiler Chicken Feeds Using Distillers Dried Grains with Solubles
Authors: Rochell Samuel J.
Published: August 2018

Distillers dried grains with solubles (DDGS) is a coproduct of corn-based ethanol production that can be a valuable source of energy, digestible amino acids, and available phosphorus in poultry feeds. Dietary incorporation of DDGS reduces the amount of primary ingredients such as corn and soybean meal needed to formulate poultry diets, improving the sustainability of both biofuel and poultry production. The nutritional value of DDGS has been extensively evaluated since it became increasingly available to feed producers in the early 2000s, but evolving methods of ethanol production and coproduct fractionation necessitate its continued characterization. Attempts to relate nutrient utilization of DDGS to its chemical composition have revealed that fiber content is a primary determinant of dietary energy value of DDGS for poultry. Distillers corn oil, which is extracted from thin stillage during production of distillers grains, can also be supplemented into poultry diets as an energy-dense lipid source in place of animal fats or other vegetable-based oils. Poultry feeding practices in the United States are also evolving, including increased adoption of all vegetable-based diets and reduced use of in-feed antimicrobials. Therefore, further characterization of both the nutritional value of DDGS and its impact on gastrointestinal health will support its continued use in poultry diets.
Materialized Genealogy: From Anonymous Cemetery Populations to Creating Alternative Narratives about Individuals and Family Burial Space
Authors: Anthony Sian
Published: August 2018

Archaeologists frequently excavate historic burials and the vast majority of the graves will be unidentified. It is rare, and also surprisingly difficult, to unite documentary sources and gravestone identities with the burials underneath. Sites are therefore interpreted and analyzed as holistic anonymous populations rather than as individual graves. Excavation of a 19th and 20th century cemetery in Copenhagen created an opportunity to explore the topic of working with identified graves through connecting to genealogical sources and theoretical approaches which are rarely encountered in archaeology. This study used alternative genealogies of grave plots based on different source materials: family trees, burial plot registers, and excavated archaeological evidence to illustrate the complementary interpretations that can be created. The research touches upon important issues of the rights and responsibilities of using the names and personal data of the dead; particularly in relation to their descendants. The conclusion is that it is vital to consider including names and sometimes personal information as doing so has deepened understanding of the variations within burial customs, the use of grave plot space and invited more personal narratives within a heavily structured system of burying the dead.
Dehumanized Victims: Analogies and Animal Avatars for Palestinian Suffering in Waltz with Bashir and “War Rabbit”
Authors: Kunert-Graf Rachel
Published: August 2018

A common convention in comics and animation is the use of animal stand-ins to provide an access point for human experiences. Whether representing anthropomorphized characters navigating very human experiences or depicting four-legged creatures impacted by human action, this strategy has the manifest intent of fostering viewer identification and empathy. In particular, artists sometimes deploy animal avatars in representations of persecution and historical trauma to avoid depicting identity categories such as race, nationality and sexuality, which constitute the ostensible basis for persecution. In this way, the use of animals to represent human suffering universalizes experiences for which difference matters. In this essay, I explore how these animal stand-ins enable or foreclose empathy with Palestinian victims in the close reading of two primary texts, “War Rabbit” and Waltz with Bashir, which employ animal avatars in place of a direct depiction of Palestinian suffering. These illustrated narratives, one a comic and one an animated film, visually rhyme animal and human suffering and verbally lament the deaths of animals. I argue that both texts fail to unpack the analogies they construct, such that these constructions ultimately represent the actual Palestinians victims as being mute and irrational. Thus this use of animal avatars, which is meant to be foster empathy, is instead oblique, and risks further dehumanizing victims and negating their experiences.
The Misogynous Politics of Shame
Authors: Bergoffen Debra
Published: August 2018

Joanna Bourke’s account of the ways that changing ideas of rape reflect the gendered norms of the times, and Eric Reitan’s proposal that rape ought to remain a contested concept amenable to evolving principles of ethical sexual relationships, speak to the ways that social, cultural, and political contexts influence our understanding of sexual violence. Though the criteria that are used to define rape change, one thing remains constant: the raped person is shamed. As she is shamed, she is degraded. This paper argues that until we understand the role that shame plays in enabling sexual violence by humiliating, silencing, and stigmatizing its victims, changes in our depictions of rape will neither disable the personal devastation of being raped nor dismantle the social practices and political institutions that rely on rape to maintain misogynous inequalities. Following the Introduction (Section 1) it is divided into three parts. Section 2, The Shame of Being Human, discusses the psychological and phenomenological accounts of shame. It alerts us to the ways that shame defines us insofar as it reveals the truth of human intersubjectivity and mutual interdependency. Section 3, Debilitating Shame, describes the ways that shame has been exploited to enable and enforce sexed and gendered inequalities. Section 4, Shame: Demanding Justice, examines the ways that shame, in its role as the protector of the self, undermines the effects of debilitating shame and fosters a politics of sexual integrity by affirming the dignity of the interdependencies that tie us to each other.
Sustainable Urbanization Performance Evaluation Based on “Origin” and “Modernization” Perspectives: A Case Study of Chongqing, China
Authors: Bian Jing , Ren Hong , Liu Ping , Zhang Yu
Published: August 2018

Although the acceleration of urbanization brings social and economic development, it also produces various problems. Accurate evaluation of sustainable urbanization performance can help local governments summarize experiences and solve problems. Sustainable urban development should focus not only on modern construction, but also on original natural ecosystems and traditional cultural protection. This paper develops a holistic framework based on an “origin” and “modernization” perspectives and uses the multilevel extension method and the Analytic Hierarchy Process (AHP) method for accurately evaluating sustainable urbanization performance. A case study of Chongqing City in China demonstrates the process of using the holistic framework and evaluation method. The empirical results from this study indicate that Chongqing has a medium level of sustainable urbanization. The city is considered as a medium level in “origin” performance and the “modernization” performance is good, while uncoordinated. The case study reveals that the proposed framework and the method are effective theoretical bases for policy-makers and practitioners to understand the performance of urban sustainability and for promoting urbanization toward better sustainability. Beyond the application case, the holistic framework and method can be applied to other cities.
A Novel Multiattribute Decision-Making Method Based on Point–Choquet Aggregation Operators and Its Application in Supporting the Hierarchical Medical Treatment System in China
Authors: Zhang Runtong , Xing Yuping , Wang Jun , Shang Xiaopu , Zhu Xiaomin
Published: August 2018

The hierarchical medical treatment system is an efficient way to solve the problem of insufficient and unbalanced medical resources in China. Essentially, classifying the different degrees of diseases according to the doctor’s diagnosis is a key step in pushing forward the hierarchical medical treatment system. This paper proposes a framework to solve the problem where diagnosis values are given as picture fuzzy numbers (PFNs). Point operators can reduce the uncertainty of doctor’s diagnosis and get intensive information in the process of decision making, and the Choquet integral operator can consider correlations among symptoms. In order to take full advantage of these two kinds of operators, in this paper, we firstly define some point operators under the picture fuzzy environment, and further propose a new class of picture fuzzy point–Choquet integral aggregation operators. Moreover, some desirable properties of these operators are also investigated in detail. Then, a novel approach based on these operators for multiattribute decision-making problems in the picture fuzzy context is introduced. Finally, we give an example to illustrate the applicability of the new approach in assisting hierarchical medical treatment system. This is of great significance for integrating the medical resources of the whole society and improving the service efficiency of the medical service system.
Caffeinated Drinks Intake, Late Chronotype, and Increased Body Mass Index among Medical Students in Chongqing, China: A Multiple Mediation Model
Authors: Zhang Yangchang , Xiong Yang , Dong Jia , Guo Tingting , Tang Xiaoman , Zhao Yong
Published: August 2018

Background: This paper investigates the problems regarding caffeinated drinks intake, late chronotype, and increased body mass index (BMI) among medical students at a Chinese university. Methods: This cross-sectional study was conducted in 2018 with 616 medical students from Chongqing Medical University in Chongqing, China, whose information were collected by a self-reported questionnaire that included four sections: Demographic characteristics; Caffeinated drinks intake and physical state; Morningness-Eveningness Questionnaire; Depression Anxiety Stress Scale 21. Multiple mediation analyses were conducted to test the impact of late chronotype on increased BMI through caffeinated drinks consumption through two models. Results: The significantly mediated effect of caffeinated drinks consumption was revealed (estimate: −0.01, SE = 0.01, 95% CI [−0.02, −0.01]), and which played a positive role in linking late chronotype (B = −0.01, SE = 0.01, p < 0.001) and increased BMI (B = 1.37, SE = 0.21, p < 0.01), but their significant association did not be found in reversed model. In addition, physical activity and inactivity times demonstrated significant indirect effects in the two models. Conclusions: Interventions should focus on reducing caffeinated drinks intake and sedentary behavior time, enhancing physical activity among medical students.
Human Polyomaviruses and Papillomaviruses
Authors: Moens Ugo
Published: August 2018

n/a
Phytochemicals in Helicobacter pylori Infections: What Are We Doing Now?
Authors: Salehi Bahare , Sharopov Farukh , Martorell Miquel , Rajkovic Jovana , Ademiluyi Adedayo Oluwaseun , Sharifi-Rad Mehdi , Fokou Patrick Valere Tsouh , Martins Natália , Iriti Marcello , Sharifi-Rad Javad
Published: August 2018

In this critical review, plant sources used as effective antibacterial agents against Helicobacter pylori infections are carefully described. The main intrinsic bioactive molecules, responsible for the observed effects are also underlined and their corresponding modes of action specifically highlighted. In addition to traditional uses as herbal remedies, in vitro and in vivo studies focusing on plant extracts and isolated bioactive compounds with anti-H. pylori activity are also critically discussed. Lastly, special attention was also given to plant extracts with urease inhibitory effects, with emphasis on involved modes of action.
Modulation of Gene Expression in Liver of Hibernating Asiatic Toads (Bufo gargarizans)
Authors: Jin Long , Yu Jian Ping , Yang Zai Jun , Merilä Juha , Liao Wen Bo
Published: August 2018

Hibernation is an effective energy conservation strategy that has been widely adopted by animals to cope with unpredictable environmental conditions. The liver, in particular, plays an important role in adaptive metabolic adjustment during hibernation. Mammalian studies have revealed that many genes involved in metabolism are differentially expressed during the hibernation period. However, the differentiation in global gene expression between active and torpid states in amphibians remains largely unknown. We analyzed gene expression in the liver of active and torpid Asiatic toads (Bufo gargarizans) using RNA-sequencing. In addition, we evaluated the differential expression of genes between females and males. A total of 1399 genes were identified as differentially expressed between active and torpid females. Of these, the expressions of 395 genes were significantly elevated in torpid females and involved genes responding to stresses, as well as contractile proteins. The expression of 1004 genes were significantly down-regulated in torpid females, most which were involved in metabolic depression and shifts in the energy utilization. Of the 715 differentially expressed genes between active and torpid males, 337 were up-regulated and 378 down-regulated. A total of 695 genes were differentially expressed between active females and males, of which 655 genes were significantly down-regulated in males. Similarly, 374 differentially expressed genes were identified between torpid females and males, with the expression of 252 genes (mostly contractile proteins) being significantly down-regulated in males. Our findings suggest that expression of many genes in the liver of B. gargarizans are down-regulated during hibernation. Furthermore, there are marked sex differences in the levels of gene expression, with females showing elevated levels of gene expression as compared to males, as well as more marked down-regulation of gene-expression in torpid males than females.
Enhanced Stretch Formability of AZ31 Magnesium Alloy Thin Sheet by Induced Precompression and Sequent Annealing
Authors: Wang Lifei , Song Bo , Zhang Zhengyong , Zhang Hua , Han Tingzhuang , Cao Xiaoqing , Wang Hongxia , Cheng Weili
Published: August 2018

In this study, precompression deformation with a strain level of 5.38% along the transverse direction (TD) at room temperature was conducted on a AZ31 magnesium alloy thin sheet with thickness of 1mm. Then subsequent annealing treatment was carried out at various temperatures (200, 300, 400, and 500 °C) to induce static recrystallization (SRX) and grain growth. The stretch formability was also investigated using the hemispherical test. The results showed that the twinning texture induced by the precompression process was nearly inherited by recrystallized grains after annealing process. Grains grew up and the size increased with the increase of annealing temperature. The largest grain size was obtained when annealing at 400 °C. The mechanical properties including strength and ductility decreased due to the development of coarse grains, however, the stretch formability was enhanced significantly. Indeed, the IE-value increased from 2.83 mm in the as-received Mg alloy sheet to 5.78 mm in the precompressed and 400 °C annealed specimens, leading to an improvement of 104%. This was ascribed to the rotated grain orientation and higher activity of (10–12) twins in coarse grains.
Enhanced Antibacterial Activity of Silver Doped Titanium Dioxide-Chitosan Composites under Visible Light
Authors: Li Jie , Xie Bing , Xia Kai , Li Yingchun , Han Jing , Zhao Chunmao
Published: August 2018

Nano titanium dioxide (TiO2) with photocatalytic activity was firstly modified by diethanolamine, and it was then doped with broad spectrum antibacterial silver (Ag) by in situ method. Further, both Ag doped TiO2-chitosan (STC) and TiO2-chitosan (TC) composites were prepared by the inverse emulsion cross-linking reaction. The antibacterial activities of STC composites were studied and their antibacterial mechanisms under visible light were investigated. The results show that in situ doping and inverse emulsion method led to good dispersion of Ag and TiO2 nanoparticles on the cross-linked chitosan microsphere. The STC with regular particle size of 1–10 μm exhibited excellent antibacterial activity against E. coli, P. aeruginosa and S. aureus under visible light. It is believed that STC with particle size of 1–10 μm has large specific surface area to contact with bacterial cell wall. The increased antibacterial activity was attributed to the enhancement of both electron-hole separations at the surface of nano-TiO2 by the silver ions under the visible light, and the synergetic and sustained release of strong oxidizing hydroxyl radicals of nano-TiO2, together with silver ions against bacteria. Thus, STC composites have great potential applications as antibacterial agents in the water treatment field.
Multiscale Modeling and Simulation of Directional Solidification Process of Ni-Based Superalloy Turbine Blade Casting
Authors: Xu Qingyan , Yang Cong , Zhang Hang , Yan Xuewei , Tang Ning , Liu Baicheng
Published: August 2018

Ni-based superalloy turbine blades have become indispensable structural parts in modern gas engines. An understanding of the solidification behavior and microstructure formation in directional solidified turbine blades is necessary for improving their high-temperature performance. The multiscale simulation model was developed to simulate the directional solidification process of superalloy turbine blades. The 3D cellular automaton-finite difference (CA-FD) method was used to calculate heat transfer and grain growth on the macroscopic scale, while the phase-field method was developed to simulate dendrite growth on the microscopic scale. Firstly, the evolution of temperature field of an aero-engine blade and a large industrial gas turbine blade was studied under high-rate solidification (HRS) and liquid-metal cooling (LMC) solidification processes. The varying withdrawal velocity was applied to change the curved mushy zone to a flat shape. Secondly, the grain growth in the aero-engine blade was simulated, and the grain structures in the starter block part and the spiral selector part in the HRS process were compared with those in the LMC process. The simulated grain structures were generally in agreement with experimental results. Finally, the dendrite growth in the typical HRS and LMC solidification process was investigated and the simulation results were compared with the experimental results in terms of dendrite morphology and primary dendritic spacing.
Metal Sequestration through Coupled Dissolution–Precipitation at the Brucite–Water Interface
Authors: Hövelmann Jörn , Putnis Christine , Benning Liane
Published: August 2018

The increasing release of potentially toxic metals from industrial processes can lead to highly elevated concentrations of these metals in soil, and ground- and surface-waters. Today, metal pollution is one of the most serious environmental problems and thus, the development of effective remediation strategies is of paramount importance. In this context, it is critical to understand how dissolved metals interact with mineral surfaces in soil–water environments. Here, we assessed the processes that govern the interactions between six common metals (Zn, Cd, Co, Ni, Cu, and Pb) with natural brucite (Mg(OH)2) surfaces. Using atomic force microscopy and a flow-through cell, we followed the coupled process of brucite dissolution and subsequent nucleation and growth of various metal bearing precipitates at a nanometer scale. Scanning electron microscopy and Raman spectroscopy allowed for the identification of the precipitates as metal hydroxide phases. Our observations and thermodynamic calculations indicate that this coupled dissolution–precipitation process is governed by a fluid boundary layer at the brucite–water interface. Importantly, this layer differs in composition and pH from the bulk solution. These results contribute to an improved mechanistic understanding of sorption reactions at mineral surfaces that control the mobility and fate of toxic metals in the environment.
Ethanolic Extract of Origanum vulgare Suppresses Propionibacterium acnes-Induced Inflammatory Responses in Human Monocyte and Mouse Ear Edema Models
Authors: Chuang Lu-Te , Tsai Tsung-Hsien , Lien Tsung-Jung , Huang Wen-Cheng , Liu Jun-Jen , Chang Hsiang , Chang Mei-Ling , Tsai Po-Jung
Published: August 2018

Acne vulgaris (acne) is a common inflammatory skin disorder, and Propionibacterium acnes plays a major role in the development and progression of acne inflammation. Herbs possessing antimicrobial and anti-inflammatory activity have been applied as a medical option for centuries. In this study, we examined the suppressive effect of ethanolic oregano (Origanum vulgare) extract on live P. acnes-induced in vivo and in vitro inflammation. Following ethanol extraction of oregano leaves, four compounds with strong antioxidant activity, including rosmarinic acid, quercetin, apigenin, and carvacrol, were identified by high-performance liquid chromatography. Using the mouse ear edema model, we demonstrated that ethanol oregano extracts (EOE) significantly suppressed P. acnes-induced skin inflammation, as measured by ear thickness (32%) and biopsy weight (37%). In a separate study, using the co-culture of P. acnes and human THP-1 monocytes, EOE reduced the production of interleukin (IL)-8, IL-1β and tumor necrosis factor (TNF)-α up to 40%, 37%, and 18%, respectively, as well as the expression of these three pro-inflammatory mediators at the transcriptional level. Furthermore, EOE inhibited the translocation of nuclear factor-kappa B (NF-κB) into the nucleus possibly by inactivating toll-like receptor-2 (TLR2). The suppressive effect of EOE on live P. acnes-induced inflammatory responses could be due, in part, to the anti-inflammatory and antioxidant properties, but not the anti-microbial effect of EOE.
Molecular Dynamics on Wood-Derived Lignans Analyzed by Intermolecular Network Theory
Authors: Sandberg Thomas Olof , Weinberger Christian , Smått Jan-Henrik
Published: August 2018

The dynamics of interactions to a solvent is a key factor in the proper characterization of new molecular structures. In molecular dynamics simulations, the solvent molecules are explicitly present, thereby defining a more accurate description on how the solvent molecules affect the molecular conformation. Intermolecular interactions in chemical systems, e.g., hydrogen bonds, can be considered as networks or graphs. Graph theoretical analyses can be an outstanding tool in analyzing the changes in interactions between solvent and solute. In this study, the software ChemNetworks is applied to interaction studies between TIP4P solvent molecules and organic solutes, i.e., wood-derived lignan-based ligands called LIGNOLs, thereby supporting the research of interaction networks between organic molecules and solvents. This new approach is established by careful comparisons to studies using previously available tools. In the hydration studies, tetramethyl 1,4-diol is found to be the LIGNOL which was most likely to form hydrogen bonds to the TIP4P solvent.
Synthesis of 2,6-Diamino-Substituted Purine Derivatives and Evaluation of Cell Cycle Arrest in Breast and Colorectal Cancer Cells
Authors: Bosco Bartolomeo , Defant Andrea , Messina Andrea , Incitti Tania , Sighel Denise , Bozza Angela , Ciribilli Yari , Inga Alberto , Casarosa Simona , Mancini Ines
Published: August 2018

Reversine is a potent antitumor 2,6-diamino-substituted purine acting as an Aurora kinases inhibitor and interfering with cancer cell cycle progression. In this study we describe three reversine-related molecules, designed by docking calculation, that present structural modifications in the diamino units at positions 2 and 6. We investigated the conformations of the most stable prototropic tautomers of one of these molecules, the N6-cyclohexyl-N6-methyl-N2-phenyl-7H-purine-2,6-diamine (3), by Density Functional Theory (DFT) calculation in the gas phase, water and chloroform, the last solvent considered to give insights into the detection of broad signals in NMR analysis. In all cases the HN(9) tautomer resulted more stable than the HN(7) form, but the most stable conformations changed in different solvents. Molecules 1–3 were evaluated on MCF-7 breast and HCT116 colorectal cancer cell lines showing that, while being less cytotoxic than reversine, they still caused cell cycle arrest in G2/M phase and polyploidy. Unlike reversine, which produced a pronounced cell cycle arrest in G2/M phase in all the cell lines used, similar concentrations of 1–3 were effective only in cells where p53 was deleted or down-regulated. Therefore, our findings support a potential selective role of these structurally simplified, reversine-related molecules in p53-defective cancer cells.
Chemical Synthesis of Rare, Deoxy-Amino Sugars Containing Bacterial Glycoconjugates as Potential Vaccine Candidates
Authors: Behera Archanamayee , Kulkarni Suvarn S.
Published: August 2018

Bacteria often contain rare deoxy amino sugars which are absent in the host cells. This structural difference can be harnessed for the development of vaccines. Over the last fifteen years, remarkable progress has been made toward the development of novel and efficient protocols for obtaining the rare sugar building blocks and their stereoselective assembly to construct conjugation ready bacterial glycans. In this review, we discuss the total synthesis of a variety of rare sugar containing bacterial glycoconjugates which are potential vaccine candidates.
Melatonin Uptake by Cells: An Answer to Its Relationship with Glucose?
Authors: Mayo Juan C. , Aguado Arturo , Cernuda-Cernuda Rafael , Álvarez-Artime Alejandro , Cepas Vanesa , Quirós-González Isabel , Hevia David , Sáinz Rosa M.
Published: August 2018

Melatonin, N-acetyl-5-methoxytryptamine, is an indole mainly synthesized from tryptophan in the pineal gland and secreted exclusively during the night in all the animals reported to date. While the pineal gland is the major source responsible for this night rise, it is not at all the exclusive production site and many other tissues and organs produce melatonin as well. Likewise, melatonin is not restricted to vertebrates, as its presence has been reported in almost all the phyla from protozoa to mammals. Melatonin displays a large set of functions including adaptation to light: dark cycles, free radical scavenging ability, antioxidant enzyme modulation, immunomodulatory actions or differentiation–proliferation regulatory effects, among others. However, in addition to those important functions, this evolutionary ‘ancient’ molecule still hides further tools with important cellular implications. The major goal of the present review is to discuss the data and experiments that have addressed the relationship between the indole and glucose. Classically, the pineal gland and a pinealectomy were associated with glucose homeostasis even before melatonin was chemically isolated. Numerous reports have provided the molecular components underlying the regulatory actions of melatonin on insulin secretion in pancreatic beta-cells, mainly involving membrane receptors MTNR1A/B, which would be partially responsible for the circadian rhythmicity of insulin in the organism. More recently, a new line of evidence has shown that glucose transporters GLUT/SLC2A are linked to melatonin uptake and its cellular internalization. Beside its binding to membrane receptors, melatonin transportation into the cytoplasm, required for its free radical scavenging abilities, still generates a great deal of debate. Thus, GLUT transporters might constitute at least one of the keys to explain the relationship between glucose and melatonin. These and other potential mechanisms responsible for such interaction are also discussed here.