bluuebunny/crossref_metadata_2025 huggingface dataset:
Reflections on a career in operational science: some lessons learned
Authors: Jolly Gill
Published: May 2023

In this short essay, I reflect on my career and some of the inspirational scientists that have influenced the way I have chosen to work. I highlight some of my lessons learnt as a woman scientist in an environment that remains largely male-dominated at a senior level. Volcanology (and science more generally) as a profession has changed for the good over the last four decades, with many early and mid-career women aiming for, and achieving, senior roles. I look forward to seeing the next-generation of diverse volcanologists drive the science forward to keep communities safer from volcanic activity.
Thyroid hormone membrane receptor binding and transcriptional regulation in the sea urchin Strongylocentrotus purpuratus
Authors: Taylor Elias , Wynen Hannah , Heyland Andreas
Published: May 2023

Thyroid hormones (THs) are small amino acid derived signaling molecules with broad physiological and developmental functions in animals. Specifically, their function in metamorphic development, ion regulation, angiogenesis and many others have been studied in detail in mammals and some other vertebrates. Despite extensive reports showing pharmacological responses of invertebrate species to THs, little is known about TH signaling mechanisms outside of vertebrates. Previous work in sea urchins suggests that non-genomic mechanisms are activated by TH ligands. Here we show that several THs bind to sea urchin (Strongylocentrotus purpuratus) cell membrane extracts and are displaced by ligands of RGD-binding integrins. A transcriptional analysis across sea urchin developmental stages shows activation of genomic and non-genomic pathways in response to TH exposure, suggesting that both pathways are activated by THs in sea urchin embryos and larvae. We also provide evidence associating TH regulation of gene expression with TH response elements in the genome. In ontogeny, we found more differentially expressed genes in older larvae compared to gastrula stages. In contrast to gastrula stages, the acceleration of skeletogenesis by thyroxine in older larvae is not fully inhibited by competitive ligands or inhibitors of the integrin membrane receptor pathway, suggesting that THs likely activate multiple pathways. Our data confirms a signaling function of THs in sea urchin development and suggests that both genomic and non-genomic mechanisms play a role, with genomic signaling being more prominent during later stages of larval development.
Long-term effects of GH therapy in adult patients with Prader-Willi syndrome: a longitudinal study
Authors: Grugni Graziano , Sartorio Alessandro , Soranna Davide , Zambon Antonella , Grugni Lucia , Zampino Giuseppe , Crinò Antonino
Published: May 2023

Introduction

Prader-Willi syndrome (PWS) is a complex disorder resulting from the failure of expression of paternal alleles in the PWS region of chromosome 15. The PWS phenotype resembles that observed in the classic non-PWS GH deficiency (GHD), including short stature, excessive fat mass, and reduced muscle mass. To date, a small number of studies on the long-term effects of GH treatment are available in adult subjects with PWS.

Methods

In this longitudinal study, 12 obese subjects with PWS (GHD/non-GHD 6/6) were treated for a median of 17 years, with a median GH dose of 0.35 mg/day. The median age was 27.1 years. Anthropometric, body composition, hormonal, biochemical, and blood pressure variables were analyzed in all subjects.

Results

Waist circumference was significantly lower at the end of the treatment period (p-value=0.0449), while body mass index (BMI) did not differ significantly. Compared to the baseline, a highly significant reduction of Fat Mass % (FM%) was observed (p-value=0.0005). IGF-I SDS values significantly increased during GH therapy (p-value=0.0005). A slight impairment of glucose homeostasis was observed after GH therapy, with an increase in the median fasting glucose levels, while insulin, HOMA-IR, and HbA1c values remained unchanged. Considering GH secretory status, both subjects with and without GHD showed a significant increase in IGF-I SDS and a reduction of FM% after GH therapy (p-value= 0.0313 for all).

Discussion

Our results indicate that long-term GH treatment has beneficial effects on body composition and body fat distribution in adults with PWS associated with obesity. However, the increase in glucose values during GH therapy should be considered, and continuous surveillance of glucose metabolism is mandatory during long-term GH therapy, especially in subjects with obesity.
Presence of 6PPD-quinone in runoff water samples from Norway using a new LC–MS/MS method
Authors: Kryuchkov Fedor , Foldvik Anders , Sandodden Roar , Uhlig Silvio
Published: May 2023

The chemical 6PPD-quinone is highly toxic to some fish species of the Oncorhynchus and Salvelinus genera and is the oxidation product of the common car tire additive 6PPD. We present a new sample preparation method that involves liquid-liquid extraction of water samples followed by silica-based solid phase extraction prior to LC–MS/MS analysis. The new sample preparation method showed good analyte recovery from spiked water samples (78%–91%) and a low ion suppression effect, surpassing previously published methods. This new method was successfully validated, achieving a limit of quantification of 5 ng/L and estimated expanded measurement uncertainty of 18.6%. In a proof-of-concept study, the method was applied to several water samples from various sources in Southern Norway. These were runoff samples from tunnel washing, from a tunnel runoff treatment plant and downstream of the plant drain. In addition, two water samples from puddles were included: one was run-off from an artificial soccer turf field and one from a puddle on a country road. The results of the analyses revealed that the concentration of 6PPD-quinone was above the LC50 reported for coho salmon (Oncorhynchus kisutch) in all samples except the samples from and downstream of the treatment plant. The highest measured concentration was 258 ng/L, which is the 2.7-fold of the reported LC50 in coho salmon (95 ng/L). Our initial data emphasize the need for more comprehensive environmental monitoring of 6PPD-quinone as well as toxicological studies in aquatic organisms.
Anti-staphylococcus aureus adaptive immunity is impaired in end-stage renal disease patients on hemodialysis: one-year longitudinal study
Authors: Darbouret- Hervier Anne , Assi Nada , Asensio Marie-Jeanne , Bernabe Beatrice , Lechevallier Aurélie , Iantomasi Raffaella , Rokbi Bachra , Botelho-Nevers Elisabeth , Ruiz Sophie
Published: May 2023

Introduction

Patients with end-stage renal disease (ESRD) display defects in adaptive and innate immunity, increasing susceptibility to infection. Staphylococcus aureus (S. aureus) is a major cause of bacteraemia in this population and is associated with increased mortality. More information on the immune response to S. aureus in these patients is needed to inform effective vaccine development.

Methods

A longitudinal prospective study was carried out at two medical centers and included 48 ESRD patients who started chronic hemodialysis (HD) treatment ≤3 months before inclusion. Control samples were taken from 62 consenting healthy blood donors. Blood samples were obtained from ESRD patients at each visit, on month (M) 0 (beginning of HD), M6 and M12. Around 50 immunological markers of adaptive and innate immunity were assessed to compare immune responses to S. aureus in ESRD patients versus controls to document the changes on their immune profile during HD.

Results

S. aureus survival in whole blood was significantly higher in ESRD patients than in controls at M0 (P=0.049), while impaired oxidative burst activity was observed in ESRD patients at all timepoints (P<0.001). S. aureus-specific immunoglobulin G (IgG) responses to iron surface determinant B (IsdB) and S. aureus α hemolysin (Hla) antigens were lower in ESRD patients than in healthy donors at M0 (P=0.003 and P=0.007, respectively) and M6 (P=0.05 and P=0.03, respectively), but were restored to control levels at M12. Moreover, S. aureus-specific T-helper cell responses were comparable to controls for IsdB but were impaired for Hla antigen at all timepoints: 10% of ESRD patients responded to Hla at M0, increasing to 30% at M12, compared with 45% of healthy donors. B-cell and T-cell concentrations in blood were significantly reduced (by 60% and 40%, respectively) compared with healthy controls. Finally, upregulation of Human Leucocyte Antigen-DR (HLA-DR) and C-C chemokine Receptor type 2 (CCR2) was impaired at M0 but was restored during the first year of HD.

Conclusion

All together, these results show that adaptive immunity was largely impaired in ESRD patients, whereas innate immunity was less impacted and tended to be restored by HD.
Coalescing disparate data sources for the geospatial prediction of mosquito abundance, using Brazil as a motivating case study
Authors: Musah Anwar , Browning Ella , Aldosery Aisha , Valerio Graciano Borges Iuri , Ambrizzi Tercio , Tunali Merve , Başibüyük Selma , Yenigün Orhan , Moreno Giselle Machado Magalhaes , de Lima Clarisse Lins , da Silva Ana Clara Gomes , dos Santos Wellington Pinheiro , Massoni Tiago , Campos Luiza Cintra , Kostkova Patty
Published: May 2023

One of the barriers to performing geospatial surveillance of mosquito occupancy or infestation anywhere in the world is the paucity of primary entomologic survey data geolocated at a residential property level and matched to important risk factor information (e.g., anthropogenic, environmental, and climate) that enables the spatial risk prediction of mosquito occupancy or infestation. Such data are invaluable pieces of information for academics, policy makers, and public health program managers operating in low-resource settings in Africa, Latin America, and Southeast Asia, where mosquitoes are typically endemic. The reality is that such data remain elusive in these low-resource settings and, where available, high-quality data that include both individual and spatial characteristics to inform the geospatial description and risk patterning of infestation remain rare. There are many online sources of open-source spatial data that are reliable and can be used to address such data paucity in this context. Therefore, the aims of this article are threefold: (1) to highlight where these reliable open-source data can be acquired and how they can be used as risk factors for making spatial predictions for mosquito occupancy in general; (2) to use Brazil as a case study to demonstrate how these datasets can be combined to predict the presence of arboviruses through the use of ecological niche modeling using the maximum entropy algorithm; and (3) to discuss the benefits of using bespoke applications beyond these open-source online data sources, demonstrating for how they can be the new “gold-standard” approach for gathering primary entomologic survey data. The scope of this article was mainly limited to a Brazilian context because it builds on an existing partnership with academics and stakeholders from environmental surveillance agencies in the states of Pernambuco and Paraiba. The analysis presented in this article was also limited to a specific mosquito species, i.e., Aedes aegypti, due to its endemic status in Brazil.
Inspiration of marine sponges to design a structure for managing the coastal hydrodynamics and protection: numerical study
Authors: Hashempour Masoumeh , Kolahdoozan Morteza
Published: May 2023

Climate changes and sediment discharge within the oceans trigger many problems, such as coastline erosion and coral reef extinction hazards. Therefore, it is crucial to control wave hydrodynamics in the desired manner to protect marine environments. To prevent the promotion of sedimentation, nature has its response. The tubular sponge is a marvelous animal. It has a perforated body and sucks nutrition and water from these perforations; then, it pumps the undigested materials out from the top outlet. In the current study, an apparatus inspired by natural tubular sponges (synthetic sponges) was designed. The computational fluid dynamics derived from the Reynolds-averaged Navier-Stokes equations and image processing technique (surfaceLIC) was deployed to study how the synthetic sponge affects the wave hydrodynamics. The results revealed that the suction of the body and outflow shielding phenomenon of one sponge reduces the wave transmission by up to ≈7%. In addition, the swing motion of the jet by wave train and effluent cloud generation causes the shear on the sponge. Therefore, the momentum exchange enhances through the water column (≈46% increase of turbulent kinetic energy). It is similar to the swing behavior of flexible vegetation. Furthermore, the surfaceLIC result revealed that the effluent cloud shape changes to a pear shape, symmetric, stretched (transition), and asymmetric by increasing the pumping discharge to 600 L/h. Observing the chute–jet phenomenon by surfaceLIC also proved the diffraction and creation of a low-velocity zone in the shadow region, which is proof of a breaking wave due to the sponge’s suction/pumping and perforated body. Consequently, it can be concluded that a synthetic sponge can act as both rigid and flexible vegetation. The synthetic sponge is anticipated to mitigate sedimentation by creating unique vortices, circulating flow, and its body shape.
Genetic analysis of dystonia-related genes in Parkinson's disease
Authors: Wang Yige , Zhao Yuwen , Pan Hongxu , Zeng Qian , Zhou Xiaoxia , Xiang Yaqin , Zhou Zhou , Xu Qian , Sun Qiying , Tan Jieqiong , Yan Xinxiang , Li Jinchen , Guo Jifeng , Tang Beisha , Yu Qiao , Liu Zhenhua
Published: May 2023

Objective

Parkinson's disease (PD) and dystonia are two closely related movement disorders with overlaps in clinical phenotype. Variants in several dystonia-related genes were demonstrated to be associated with PD; however, genetic evidence for the involvement of dystonia-related genes in PD has not been fully studied. Here, we comprehensively investigated the association between rare variants in dystonia-related genes and PD in a large Chinese cohort.

Methods

We comprehensively analyzed the rare variants of 47 known dystonia-related genes by mining the whole-exome sequencing (WES) and whole-genome sequencing (WGS) data from 3,959 PD patients and 2,931 healthy controls. We initially identified potentially pathogenic variants of dystonia-related genes in patients with PD based on different inheritance models. Sequence kernel association tests were conducted in the next step to detect the association between the burden of rare variants and the risk for PD.

Results

We found that five patients with PD carried potentially pathogenic biallelic variants in recessive dystonia-related genes including COL6A3 and TH. Additionally, we identified 180 deleterious variants in dominant dystonia-related genes based on computational pathogenicity predictions and four of which were considered as potentially pathogenic variants (p.W591X and p.G820S in ANO3, p.R678H in ADCY5, and p.R458Q in SLC2A1). A gene-based burden analysis revealed the increased burden of variant subgroups of TH, SQSTM1, THAP1, and ADCY5 in sporadic early-onset PD, whereas COL6A3 was associated with sporadic late-onset PD. However, none of them reached statistical significance after the Bonferroni correction.

Conclusion

Our findings indicated that rare variants in several dystonia-related genes are suggestively associated with PD, and taken together, the role of COL6A3 and TH genes in PD is highlighted.
Reliability, validity and normal ranges of the Zur Balance Scale for detecting mild postural control differences: introducing the modified, short version mZBS
Authors: Zur Oz , Ben-Rubi Shimron Hadas , Deutsch Lisa , Carmeli Eli
Published: May 2023

Introduction

Balance is achieved through interactions between the vestibular, somatosensory, and visual systems. There are several clinical tests to measure postural stability. However, most of them do not assess postural stability with head movements, which is the main function of the vestibular system, and those that do, require the use of sizeable, expensive equipment. Therefore, an applicable, easy-to-perform test that challenges the function of the visual, somatosensory and vestibular systems, using head movements, is needed. The Zur Balance Scale (ZBS) contains ten conditions, which are a combination of surfaces (floor or Styrofoam with subject standing on its width in Romberg position or its length in tandem position), stances (Romberg or tandem), tasks (no head movement with eyes open or closed and horizontal or vertical head movements with eyes open). The purpose of this study was to determine the validity, inter- and intra-examiner reliability, and normal performance values of the ZBS among individuals 29–70-years of age and to introduce the modified version: the mZBS, using kinetic measurements.

Methods

Healthy participants ages 29–70 years were evaluated for inter- and intra-tester reliability (n = 65), kinetic measurements on a force plate, and validity compared to the modified clinical test of sensory interaction and balance (mCTSIB) (n = 44) and characterization of normal values (n = 251).

Results

Zur Balance Scale head movements, duration of each condition (up to 10 s) and the total ZBS score agreed across examiners (ICC > 0.8). Normal ZBS scores were negatively correlated with age (r = −0.34; P < 0.0001). Older subjects (60–70 years) had a median score of 95.5 compared with younger subjects, where medians ranged from 97.6 to 98.9. Kinetic parameters showed positive correlations between ZBS and the mCTSIB scores, with the highest correlation between the five Romberg tasks (modified ZBS).

Conclusion

Zur Balance Scale is a valid and reliable test. Its advantages include using head movements and the ability to detect minimal differences in postural control, even in healthy populations. Kinetic evaluation of the ZBS enables the use of a modified, shorter version of the ZBS (mZBS).
A garment that measures brain activity: proof of concept of an EEG sensor layer fully implemented with smart textiles
Authors: López-Larraz Eduardo , Escolano Carlos , Robledo-Menéndez Almudena , Morlas Leyre , Alda Alexandra , Minguez Javier
Published: May 2023

This paper presents the first garment capable of measuring brain activity with accuracy comparable to that of state-of-the art dry electroencephalogram (EEG) systems. The main innovation is an EEG sensor layer (i.e., the electrodes, the signal transmission, and the cap support) made entirely of threads, fabrics, and smart textiles, eliminating the need for metal or plastic materials. The garment is connected to a mobile EEG amplifier to complete the measurement system. As a first proof of concept, the new EEG system (Garment-EEG) was characterized with respect to a state-of-the-art Ag/AgCl dry-EEG system (Dry-EEG) over the forehead area of healthy participants in terms of: (1) skin-electrode impedance; (2) EEG activity; (3) artifacts; and (4) user ergonomics and comfort. The results show that the Garment-EEG system provides comparable recordings to Dry-EEG, but it is more susceptible to artifacts under adverse recording conditions due to poorer contact impedances. The textile-based sensor layer offers superior ergonomics and comfort compared to its metal-based counterpart. We provide the datasets recorded with Garment-EEG and Dry-EEG systems, making available the first open-access dataset of an EEG sensor layer built exclusively with textile materials. Achieving user acceptance is an obstacle in the field of neurotechnology. The introduction of EEG systems encapsulated in wearables has the potential to democratize neurotechnology and non-invasive brain-computer interfaces, as they are naturally accepted by people in their daily lives. Furthermore, supporting the EEG implementation in the textile industry may result in lower cost and less-polluting manufacturing processes compared to metal and plastic industries.
Intraneuronal accumulation of amyloid-β peptides as the pathomechanism linking autism and its co-morbidities: epilepsy and self-injurious behavior — the hypothesis
Authors: Frackowiak Janusz , Mazur-Kolecka Bozena
Published: May 2023

Autism spectrum disorder (ASD) is associated with enhanced processing of amyloid-β precursor protein (APP) by secretase-α, higher blood levels of sAPPα and intraneuronal accumulation of N-terminally truncated Aβ peptides in the brain cortex — mainly in the GABAergic neurons expressing parvalbumin — and subcortical structures. Brain Aβ accumulation has been also described in epilepsy—the frequent ASD co-morbidity. Furthermore, Aβ peptides have been shown to induce electroconvulsive episodes. Enhanced production and altered processing of APP, as well as accumulation of Aβ in the brain are also frequent consequences of traumatic brain injuries which result from self-injurious behaviors, another ASD co-morbidity. We discuss distinct consequences of accumulation of Aβ in the neurons and synapses depending on the Aβ species, their posttranslational modifications, concentration, level of aggregation and oligomerization, as well as brain structures, cell types and subcellular structures where it occurs. The biological effects of Aβ species which are discussed in the context of the pathomechanisms of ASD, epilepsy, and self-injurious behavior include modulation of transcription—both activation and repression; induction of oxidative stress; activation and alteration of membrane receptors’ signaling; formation of calcium channels causing hyper-activation of neurons; reduction of GABAergic signaling — all of which lead to disruption of functions of synapses and neuronal networks. We conclude that ASD, epilepsy, and self-injurious behaviors all contribute to the enhanced production and accumulation of Aβ peptides which in turn cause and enhance dysfunctions of the neuronal networks that manifest as autism clinical symptoms, epilepsy, and self-injurious behaviors.
Real Life Clinical Management and Survival in Advanced Cutaneous Melanoma: The Italian Clinical National Melanoma Registry Experience
Authors: Crispo Anna , Corradin Maria Teresa , Giulioni Erika , Vecchiato Antonella , Del Fiore Paolo , Queirolo Paola , Spagnolo Francesco , Vanella Vito , Caracò Corrado , Tosti Giulio , Pennacchioli Elisabetta , Giudice Giuseppe , Nacchiero Eleonora , Quaglino Pietro , Ribero Simone , Giordano Monica , Marussi Desire , Barruscotti Stefania , Guida Michele , De Giorgi Vincenzo , Occelli Marcella , Grosso Federica , Cairo Giuseppe , Gatti Alessandro , Massa Daniela , Atzori Laura , Calvani Nicola , Fabrizio Tommaso , Mastrangelo Giuseppe , Toffolutti Federica , Celentano Egidio , Budroni Mario , Gandini Sara , Rossi Carlo Riccardo , Testori Alessandro , Palmieri Giuseppe , Ascierto Paolo A. ,   the Clinical National Melanoma Registry Study Group at the Italian Melanoma Intergroup
Published: May 2023

Background

Cutaneous melanoma (CM) is one of the most aggressive types of skin cancer. Currently, innovative approaches such as target therapies and immunotherapies have been introduced in clinical practice. Data of clinical trials and real life studies that evaluate the outcomes of these therapeutic associations are necessary to establish their clinical utility. The aim of this study is to investigate the types of oncological treatments employed in the real-life clinical management of patients with advanced CM in several Italian centers, which are part of the Clinical National Melanoma Registry (CNMR).

Methods

Melanoma-specific survival and overall survival were calculated. Multivariate Cox regression models were used to estimate the hazard ratios adjusting for confounders and other prognostic factors.

Results

The median follow-up time was 36 months (range 1.2-185.1). 787 CM were included in the analysis with completed information about therapies. All types of immunotherapy showed a significant improved survival compared with all other therapies (p=0.001). 75% was the highest reduction of death reached by anti-PD-1 (HR=0.25), globally immunotherapy was significantly associated with improved survival, either for anti-CTLA4 monotherapy or combined with anti-PD-1 (HR=0.47 and 0.26, respectively) and BRAFI+MEKI (HR=0.62).

Conclusions

The nivolumab/pembrolizumab in combination of ipilimumab and the addition of ant-MEK to the BRAFi can be considered the best therapies to improve survival in a real-world-population. The CNMR can complement clinical registries with the intent of improving cancer management and standardizing cancer treatment.
Prospective monitoring of chronic myeloid leukemia patients from the time of TKI discontinuation: the fate of peripheral blood CD26+ leukemia stem cells
Authors: Pacelli Paola , Santoni Adele , Sicuranza Anna , Abruzzese Elisabetta , Giai Valentina , Crugnola Monica , Annunziata Mario , Galimberti Sara , Iurlo Alessandra , Luciano Luigiana , Sorà Federica , Fava Carmen , Bestoso Elena , Marzano Cristina , Cartocci Alessandra , Defina Marzia , Sammartano Vincenzo , Cencini Emanuele , Raspadori Donatella , Bocchia Monica
Published: May 2023

Introduction: In chronic myeloid leukemia (CML), about half of the patients achieving a deep and stable molecular response with tyrosine kinase inhibitors (TKIs) may discontinue TKI treatment without disease recurrence. As such, treatment-free remission (TFR) has become an ambitious goal of treatment. Given the evidence that deepness and duration of molecular response are necessary but not sufficient requisites for a successful TFR, additional biological criteria are needed to identify CML patients suitable for efficacious discontinuation. Leukemia stem cells (LSCs) are supposed to be the reservoir of the disease. Previously, we demonstrated that residual circulating CD34+/CD38-/CD26+ LSCs were still detectable in a consistent number of CML patients during TFR.

Methods: CML LSCs could be easily identified by flow-cytometry as they express the CD34+/CD38-/CD26+ phenotype. In this study, we explored the role of these cells and their correlation with molecular response in a cohort of 109 consecutive chronic phase CML patients prospectively monitored from the time of TKI discontinuation.

Results: After a median observation time of 33 months from TKI discontinuation, 38/109 (35%) patients failed TFR after a median time of 4 months, while 71/109 (65%) patients are still in TFR. At TKI discontinuation, peripheral blood CD26+LSCs were undetectable in 48/109 (44%) patients and detectable in 61/109 (56%). No statistically significant correlation between detectable/undetectable CD26+LSCs and the rate of TFR loss was found (p = 0.616). The incidence of TFR loss based on the type of TKI treatment was statistically significant for imatinib treatment compared to that of nilotinib (p = 0.039). Exploring the behavior of CD26+LSCs during TFR, we observed fluctuating values that were very variable between patients, and they were not predictive of TFR loss.

Discussion: Up to date, our results confirm that CD26+LSCs are detectable at the time of TKI discontinuation and during TFR. Moreover, at least for the observation median time of the study, the persistence of “fluctuating” values of residual CD26+LSCs does not hamper the possibility to maintain a stable TFR. On the contrary, even patients discontinuing TKI with undetectable CD26+LSCs could undergo TFR loss. Our results suggest that factors other than residual LSCs “burden” playing an active role in controlling disease recurrence. Additional studies evaluating CD26+LSCs’ ability to modulate the immune system and their interaction in CML patients with very long stable TFR are ongoing.
Structural material nuclear data basic research
Authors: Avrigeanu Marilena , Avrigeanu Vlad
Published: May 2023

The nuclear data evaluation for deuteron-induced reactions andα-particle emission by neutron interactions is addressed within “Nuclear data for fusion technology, from basic research to full-scale applications.” The status and open questions related to these subjects in the area of nuclear data for fusion technology, specifically for the nuclear design of the ITER fusion device, the European DEMO fusion reactor, and the IFMIF-DONES Irradiation Facility, are briefly reviewed. A firm demand for accurate cross-sections of reactions induced by neutrons and deuterons exists, in this respect, within a more enlarged energy range up to 50 MeV than for fission applications. The current requirements are closely met by the TENDL Evaluated Nuclear Data Library, settled using the TALYS nuclear model code, which is one of the most widely used codes in basic research and applications including nuclear fusion technology. However, further improvement of this data library has recently been suggested, while, with respect to fission applications, not only the aforementioned energy range but also the diversity of nuclear data for fusion technologies is plainly stretched. Consequently, the progress of nuclear data activities conducted more recently on deuteron-induced reactions andα-emission by neutron interactions, throughout the European Fusion Program and subsequent to previous achievements within F4E and EUROfusion programs, is wholly summarized.
Sprint skating profile of competitive male bandy players: determination of positional differences and playing level
Authors: van den Tillaar Roland , Pojskic Haris , Andersson Håkan
Published: May 2023

This study aimed to compare sprint skating profile characteristics of the different playing positions of junior and senior bandy players. In total, 111 male national-level bandy players (age: 20.7 ± 5.0 years, height: 1.80 ± 0.05 m, body mass: 76.4 ± 0.4 kg, training experience: 13.8 ± 5.0 yrs) were tested on their sprint skating profile over 80 m. The main findings were that no differences between positions were found in sprint skating performance (speed and acceleration), but that elite players were in general heavier (p < 0.05) than junior players (80.0 ± 7.1 vs. 73.1 ± 8.1 kg), they could accelerate faster (2.96 ± 0.22 vs. 2.81 ± 0.28 m/s2), and they reached a higher velocity (10.83 ± 0.37 vs. 10.24 ± 0.42 m/s) earlier over 80 m than the junior players. This implies that junior level players should spend more time in power and sprint training to meet the specific demands of playing at a higher, elite level.
The study of the effectiveness of design-based engineering learning: the mediating role of cognitive engagement and the moderating role of modes of engagement
Authors: Wei Lina , Zhang Wei , Lin Chenhua
Published: May 2023

Aim

Design-based engineering learning (DBEL) offers a potentially valuable approach to engineering education, but its mechanism of action has yet to be verified by empirical studies. Accordingly, the present study aimed to establish whether DBEL produces better learning outcomes, thereby building a strong, empirically grounded case for further research into engineering education.

Methods

To build a more comprehensive model of design-based engineering learning, the variables of cognitive engagement (the mediator) and modes of engagement (the moderator) were introduced to build a theoretical process model. Questionnaires and multiple linear regression analysis were used to verify the model.

Results and discussion

All four features of DBEL (design practice, interactive reflection, knowledge integration, and circular iteration) were found to exert significant and positive effects on learning outcomes. Moreover, cognitive engagement was found to both fully and partially mediate the relationships between these features and the outcomes of engineering learning; under two different modes of engagement, the positive effects of the learning features on cognitive engagement differed significantly.

Conclusion

The paper concluded the following: (1) a design-based learning approach can enhance engineering students’ learning outcomes, (2) cognitive engagement mediates between design-based engineering learning and learning outcomes (3) a systematic mode of engagement produces better learning outcomes than a staged modes of engagement.
Predictors of job satisfaction among teachers in Germany during the SARS-CoV-2 pandemic: cross-sectional results of a nationwide online questionnaire
Authors: Dicks Theresa , Eggert Viktoria , Koestner Clemens , Zähme Carolina , Beutel Till , Kalo Kristin , Letzel Stephan , Dietz Pavel
Published: May 2023

Introduction

During the SARS-CoV-2 pandemic teaching was changed several times to distance learning. To consider the associated stresses and challenges for teachers a nationwide cross-sectional study was performed in March 2021 in which N = 31,089 teachers from Germany participated.

Methods

A multiple linear regression model with stepwise inclusion of thematically sorted variables (sociodemographic, SARS-CoV-2- and work-related variables) was used to identify relevant predictors of job satisfaction.

Results

The analysis revealed that work-related variables were significant predictors of job satisfaction. In the third regression model, when all variables are included the adjusted R2 was 0.364. Overall, the results showed that, e.g., work predictability (b = 0.097), influence at work (b = 0.118), and meaning of work (b = 0.212) increased job satisfaction. In contrast, increased emotional exhaustion (b = −0.016), feelings of unfair treatment (b = −0.048), and work family conflicts (b = −0.154) deteriorated job satisfaction.

Discussion

The results indicate that future research should focus especially the work-related topics in more detail and that job satisfaction is a useful concept for analyzing working conditions from a public health point of view.
Two-sample Mendelian randomization analysis evaluates causal associations between inflammatory bowel disease and osteoporosis
Authors: Dai Zhujiang , Xu Weimin , Ding Rui , Peng Xiang , Shen Xia , Song Jinglue , Du Peng , Wang Zhongchuan , Liu Yun
Published: May 2023

Introduction

Over the past few years, multiple observational studies have speculated a potential association between inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn’s disease (CD), and osteoporosis. However, no consensus has been reached regarding their interdependence and pathogenesis. Herein, we sought to further explore the causal associations between them.

Methods

We validated the association between IBD and reduced bone mineral density in humans based on genome-wide association studies (GWAS) data. To investigate the causal relationship between IBD and osteoporosis, we performed a two-sample Mendelian randomization study using training and validation sets. Genetic variation data for IBD, CD, UC, and osteoporosis were derived from published genome-wide association studies in individuals of European ancestry. After a series of robust quality control steps, we included eligible instrumental variables (SNPs) significantly associated with exposure (IBD/CD/UC). We adopted five algorithms, including MR Egger, Weighted median, Inverse variance weighted, Simple mode, and Weighted mode, to infer the causal association between IBD and osteoporosis. In addition, we evaluated the robustness of Mendelian randomization analysis by heterogeneity test, pleiotropy test, leave-one-out sensitivity test, and multivariate Mendelian randomization.

Results

Genetically predicted CD was positively associated with osteoporosis risk, with ORs of 1.060 (95% CIs 1.016, 1.106; p = 0.007) and 1.044 (95% CIs 1.002, 1.088; p = 0.039) for CD in the training and validation sets, respectively. However, Mendelian randomization analysis did not reveal a significant causal relationship between UC and osteoporosis (p > 0.05). Furthermore, we found that overall IBD was associated with osteoporosis prediction, with ORs of 1.050 (95% CIs 0.999, 1.103; p = 0.055) and 1.063 (95% CIs 1.019, 1.109; p = 0.005) in the training and validation sets, respectively.

Conclusion

We demonstrated the causal association between CD and osteoporosis, complementing the framework for genetic variants that predispose to autoimmune disease.
Associations of dietary fiber intake with chronic inflammatory airway diseases and mortality in adults: a population-based study
Authors: Lin Shanhong , Zhu Ning , Zhang Shengmin
Published: May 2023

Objective

The objective of this study was to investigate the potential association between dietary fiber intakes and the prevalence of chronic inflammatory airway diseases (CIAD), as well as mortality in participants with CIAD.

Methods

Data was collected from the National Health and Nutrition Examination Survey (NHANES) 2013–2018, with dietary fiber intakes being calculated as the average of two 24-h dietary reviews and divided into four groups. CIAD included self-reported asthma, chronic bronchitis, and chronic obstructive pulmonary disease (COPD). Through December 31, 2019, mortality was identified from the National Death Index. In cross-sectional studies, multiple logistic regressions were used to assess dietary fiber intakes associated with the prevalence of total and specific CIAD. Dose–response relationships were tested using restricted cubic spline regression. In prospective cohort studies, cumulative survival rates were calculated using the Kaplan–Meier method and compared using log-rank tests. Multiple COX regressions were used to assess dietary fiber intakes associated with mortality in participants with CIAD.

Results

A total of 12,276 adults were included in this analysis. The participants had a mean age of 50.70 ± 17.4 years and was 47.2% male. The prevalence of CIAD, asthma, chronic bronchitis, and COPD were 20.1, 15.2, 6.3, and 4.2%, respectively. The median daily consumption of dietary fiber was 15.1 [IQR 10.5, 21.1] g. After adjusting for all confounding factors, linear and negative associations were observed between dietary fiber intakes and the prevalence of total CIAD (OR = 0.68 [0.58–0.80]), asthma (OR = 0.71 [0.60–0.85]), chronic bronchitis (OR = 0.57 [0.43–0.74]) and COPD (OR = 0.51 [0.34–0.74]). In addition, the fourth quartile of dietary fiber intake levels remained significantly associated with a decreased risk of all-cause mortality (HR = 0.47 [0.26–0.83]) compared to the first quartile.

Conclusion

Dietary fiber intakes were found to be correlated with the prevalence of CIAD, and higher dietary fiber intakes were associated with a reduced mortality in participants with CIAD.
Liquid Nitrogen Flow Boiling Critical Heat Flux in Additively Manufactured Cooling Channels
Authors: Ortega Debra , Amador Alejandro , Ahmad Mohiuddin , Choudhuri Ahsan , Rahman Md Mahamudur
Published: May 2023

This paper presents an experimental characterization of liquid nitrogen (LN2) flow boiling in additively manufactured minichannels. There is a pressing need of concerted efforts from the space exploration and thermal transport communities to design high-performance rocket engine cooling channels. A close observation of the literature gaps warrants a systematic cryogenic flow boiling characterization of asymmetrically heated small (<3 mm) non-circular channels fabricated with advanced manufacturing technologies at mass flux > 3000 kg/m2s and pressure > 1 MPa. As such, this work presents the LN2 flow boiling results for three asymmetrically heated additively manufactured GR-Cop42 channels of 1.8 mm, 2.3 mm, and 2.5 mm hydraulic diameters. Twenty different tests have been performed at mass flux~3805–14,295 kg/m2s, pressures~1.38 and 1.59 MPa, and subcooling~0 and 5 K. A maximum departure from nucleate boiling (DNB)-type critical heat flux (CHF) of 768 kW/m2 has been achieved for the 1.8 mm channel. The experimental results show that CHF increases with increasing LN2 flow rate (337–459 kW/m2 at 25–57 cm3/s for 2.3 mm channel) and decreasing channel size (307–768 kW/m2 for 2.5–1.8 mm channel). Finally, an experimental DNB correlation has been developed with 10.68% mean absolute error.
Long-Term Chemical and Organic Fertilization Differently Affect Soil Aggregates and Associated Carbon and Nitrogen in the Loess Plateau of China
Authors: Yang Caidi , Sainju Upendra M. , Li Chao , Fu Xin , Zhao Fazhu , Wang Jun
Published: May 2023

Fertilizer sources may have variable effects on soil aggregation, aggregated-associated C and N, and wheat yield. A 34-year field experiment was performed to evaluate the influences of chemical and organic fertilization on soil aggregates and associated carbon and nitrogen under winter wheat in a Cumulic Haplustoll of the Loess Plateau, China. Treatments included unfertilized control (CK), inorganic N fertilizer (NF), inorganic P fertilizer (PF), inorganic N and P fertilizer (NP), organic manure (M), inorganic N fertilizer plus manure (NM), inorganic P fertilizer plus manure (PM), and inorganic N plus P fertilizers plus manure (NPM). Compared to CK, long-term fertilization significantly increased the proportion of soil macro-aggregates, mean weight diameter (MWD), and geometric mean diameter (GMD), but decreased the proportion of micro-aggregates and fractal dimension, especially fertilizer plus manure. Compared to CK, manure treatments (M, NM, PM, and NPM) had a better improvement on soil organic carbon (SOC), soil total nitrogen (STN), particle organic C, and microbial biomass C in all aggregates than the fertilizer alone. The SOC in different aggregates increased with the increased aggregate size, which was because the larger aggregates formed by the binding of the smaller aggregates and organic matter. PON increased in NM and NPM, and MBN was more sensitive to N fertilizer. The C/N ratio in bulk soil and aggregates decreased with fertilization, especially in fertilizer with manure and in macro-aggregates. The improved soil structure was related to the increased SOC and STN, which was proved by the positive correlations among SOC and STN with macro-aggregates and MWD. A correlation analysis also showed that the contribution rate of SOC and STN in macro-aggregates was positively associated with the macro-aggregate and stability. Therefore, the sequestration of C and N in soil was related to aggregate size and was mainly affected by larger aggregates. The results demonstrated that fertilizer with manure improved the soil structure and fertility better than fertilizer alone, thus increasing crop yield.
Determination of Feed Yield and Quality Parameters of Bermudagrass (Cynodon dactylon L. (Pers.)) Populations Collected from Natural Flora
Authors: Severoglu Sedat , Gullap M. Kerim
Published: May 2023

A two-year study (2020–2021) was conducted in the Erzurum province to investigate the feed quality parameters of 102 genotypes of Bermudagrass (Cynodon dactylon) collected from different locations in natural flora. Two control cultivars were also examined in this study, namely Coastal and Survivor. Parameters such as the fresh herbage weight, dry herbage weight, crude protein (CP) ratio, acid detergent fiber (ADF) ratio, neutral detergent fiber (NDF) ratio, and relative feed values (RFVs) were analyzed. In the first year of this study, Bermudagrass populations collected from natural flora were rooted in pots under greenhouse conditions. In the second year, the rooted plants were transplanted into experimental fields at the Atatürk University Plant Production Implementation and Research Center. The present genotypes generally had superior crude protein, ADF, NDF ratios, and relative feed values (RFVs) than the control cultivar. The fresh herbage weights ranged from 9.20 to 95.37 g per plant, while the dry herbage weights varied from 5.22 to 45.24 g per plant. The findings of this study showed that most of the genotypes collected from natural flora had superior feed quality parameters to the control genotypes.
High Prevalence of Microsporidia in the North African Hedgehog (Atelerix algirus) in the Canary Islands, Spain
Authors: Baz-González Edgar , Abreu-Acosta Néstor , Foronda Pilar
Published: May 2023

Microsporidia are unicellular eukaryotic obligate intracellular parasites with a wide range of hosts reported worldwide; however, little is known about the epidemiological data on microsporidia infection in animals from the Canary Islands. Since data on microsporidia infection in hedgehog species are scarce, the aim of this study was to analyze the presence and identity of microsporidia in a group of North African hedgehogs (Atelerix algirus) using microscopic and molecular methods. From December 2020 to September 2021, a total of 36 fecal samples were collected from naturally deceased hedgehogs from Tenerife and Gran Canaria. All samples showed spore-compatible structures (100%; 36/36) under microscopic analysis, of which 61.1% (22/36) were amplified via the nested-polymerase chain reaction (PCR) targeting the partial sequence of the 16S rRNA gene, the internal transcribed spacer (ITS) region, and the partial sequence of the 5.8S rRNA gene. After Sanger sequencing and ITS analysis, Enterocytozoon bieneusi was detected in 47.2% (17/36) of the samples, identifying two novel genotypes (AAE1 and AAE2), followed by the detection of an undetermined species in 8.3% (3/36) and Encephalitozoon cuniculi genotype I in 5.6% (2/36) of the samples. This study constitutes the first report of microsporidia species in Atelerix algirus worldwide, highlighting the high prevalence of zoonotic species.
Effect of CXCL17 on Subcutaneous Preadipocytes Proliferation in Goats
Authors: Lu Guangyu , Ma Xiaotong , Wang Fei , Chen Dingshuang , Lin Yaqiu , Wang Youli , Liu Wei , Li Yanyan
Published: May 2023

The presence or absence of subcutaneous adipose accumulation will affect the energy storage, insulation resistance and metabolism of animals. Proliferation and differentiation of preadipocytes play a significant role in lipid deposition. The objective of this study was to clone the goat CXCL17 gene and investigate its potential functions on goat subcutaneous preadipocytes’ proliferation by gaining or losing function in vitro. The goat CXCL17 gene was cloned by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and bioinformatics analysis was performed. The expression of the CXCL17 gene in the different goat tissues and adipocytes at different differentiation stages was detected by real-time fluorescence quantitative PCR (qPCR). The results showed that the cloned sequence of goat CXCL17 gene is 728 bp and the CDS region is 357 bp, encoding 118 amino acids. CXCL17 protein is located in nucleus, cytoplasm, mitochondria and extracellular matrix. Tissue-expression profiles revealed that CXCL17 expressed in all of the examined tissues. In visceral tissues, the highest expression level was found in lung (p < 0.01); in muscle tissues, the highest CXCL17 expression level was found in the longissimus dorsi (p < 0.01) and in adipose tissues, the highest expression level was found in subcutaneous adipose (p <0.01). Compared with those cells before differentiation, CXCL17 expression levels upregulated at 48 h (p < 0.01), 72 h (p < 0.01), 120 h (p < 0.01) and downregulated at 96 h (p < 0.01). Furthermore, the results of crystal violet staining and semi-quantitative assay showed that transfection with 1 μg CXCL17 expression plasmid reduced the cell numbers in vitro. Meanwhile, the expression of CCND1 was significantly decreased. A similar consequence happened after interfering with CXCL17 expression. However, plasmid transfected with 2 μg pEGFPN1-CXCL17 increased the number of cells in vitro. These results suggest that CXCL17 is involved in the proliferation of goat subcutaneous preadipocytes.