bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity and Bioactivity of Marine Bacteria Associated with the Sponges Candidaspongia flabellata and Rhopaloeides odorabile from the Great Barrier Reef in Australia
Authors: Brinkmann Candice , Kearns Philip , Evans-Illidge Elizabeth , Kurtbӧke D.
Published: September 2017

Sponges and their associated microbial communities have sparked much interest in recent decades due on the abundant production of chemically diverse metabolites that in nature serve as functional compounds required by the marine sponge host. These compounds were found to carry therapeutic importance for medicinal applications. In the presented study, 123 bacterial isolates from the culture collection of the Australian Institute of Marine Science (AIMS) previously isolated from two different sponge species, namely Candidaspongia flabellata and Rhopaloeides odorabile, originating from different locations on the Great Barrier Reef in Queensland, Australia, were thus studied for their bioactivity. The symbiotic bacterial isolates were first identified using 16S rRNA gene analysis and they were found to belong to five different dominating classes of Domain Bacteria, namely Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Bacilli and Actinobacteria. Following their taxonomical categorization, the isolates were screened for their antimicrobial activity against human pathogenic microbial reference strains: Escherichia coli (ATCC® BAA-196™), E. coli (ATCC® 13706™), E. coli (ATCC® 25922™), Klebsiella pneumoniae (ATCC® BAA-1705™), Enterococcus faecalis (ATCC® 51575™), Bacillus subtilis (ATCC® 19659™), Staphylococcus aureus (ATCC® 29247™), Candida albicans (ATCC® 10231™) and Aspergillus niger (ATCC® 16888™). Over 50% of the isolates displayed antimicrobial activity against one or more of the reference strains tested. The subset of these bioactive bacterial isolates was further investigated to identify their biosynthetic genes such as polyketide synthase (PKS) type I and non-ribosomal peptide synthetase (NRPS) genes. This was done using polymerase chain reaction (PCR) with degenerate primers that have been previously used to amplify PKS-I and NRPS genes. These specific genes have been reported to be possibly involved in bacterial secondary metabolite production. In 47% of the bacterial isolates investigated, the PKS and NRPS genes were located. Some of the bacterial isolates were found to possess both gene types, which agrees with the previous reported biosynthetic ability of certain sponge-symbiotic bacteria such as the Actinobacteria or Gammaproteobacteria to produce secondary metabolites with antimicrobial activity. All these reported activities further confirm that sponge-symbiotic bacteria hold significant bioactivity with medicinal and biotechnological importance.
An Improved Power Law for Nonlinear Least-Squares Fitting?
Authors: Helyer Benjamin , Courtney Michael
Published: September 2017

Models based on a power law are prevalent in many areas of study. When regression analysis is performed on data sets modeled by a power law, the traditional model uses a lead coefficient. However, the proposed model replaces the lead coefficient with a scaling parameter and reduces uncertainties in best-fit parameters for data sets with exponents close to 3. This study extends previous work by testing each model for a range of parameters. Data sets with known values of scaling parameter and exponent were generated by adding normally distributed random errors with controlled mean and standard deviations to underlying power laws. These data sets were then analyzed for both forms of the power law. For the scaling parameter, the proposed model provided smaller errors in 96/180 cases and smaller uncertainties in 88/180 cases. In most remaining cases, the traditional model provided smaller errors or uncertainties. Examination of conditions indicates that the proposed law has potential in select cases, but due to ambiguity in the conditions which favor one model over the other, an approach similar to the one in this study is encouraged for determining which model will offer reduced errors and uncertainties in data sets where additional accuracy is desired.
Recent Progress in the Development of Diagnostic Tests for Malaria
Authors: Krampa Francis , Aniweh Yaw , Awandare Gordon , Kanyong Prosper
Published: September 2017

The impact of malaria on global health has continually prompted the need to develop effective diagnostic strategies. In malaria endemic regions, routine diagnosis is hampered by technical and infrastructural challenges to laboratories. These laboratories lack standard facilities, expertise or diagnostic supplies; thus, therapy is administered based on clinical or self-diagnosis. There is the need for accurate diagnosis of malaria due to the continuous increase in the cost of medication, and the emergence and spread of drug resistant strains. However, the widely utilized Giemsa-stained microscopy and immunochromatographic tests for malaria are liable to several drawbacks, including inadequate sensitivity and false-positive outcomes. Alternative methods that offer improvements in performance are either expensive, have longer turnaround time or require a level of expertise that makes them unsuitable for point-of-care (POC) applications. These gaps necessitate exploration of more efficient detection techniques with the potential of POC applications, especially in resource-limited settings. This minireview discusses some of the recent trends and new approaches that are seeking to improve the clinical diagnosis of malaria.
Calcium Sulfoaluminate, Geopolymeric, and Cementitious Mortars for Structural Applications
Authors: Mobili Alessandra , Belli Alberto , Giosuè Chiara , Telesca Antonio , Marroccoli Milena , Tittarelli Francesca
Published: September 2017

This paper deals with the study of calcium sulfoaluminate (CSA) and geopolymeric (GEO) binders as alternatives to ordinary Portland cement (OPC) for the production of more environmentally-friendly construction materials. For this reason, three types of mortar with the same mechanical strength class (R3 ≥ 25 MPa, according to EN 1504-3) were tested and compared; they were based on CSA cement, an alkaline activated coal fly ash, and OPC. Firstly, binder pastes were prepared and their hydration was studied by means of X-ray diffraction (XRD) and differential thermal-thermogravimetric (DT-TG) analyses. Afterwards, mortars were compared in terms of workability, dynamic modulus of elasticity, adhesion to red clay bricks, free and restrained drying shrinkage, water vapor permeability, capillary water absorption, and resistance to sulfate attack. DT-TG and XRD analyses evidenced the main reactive phases of the investigated binders involved in the hydration reactions. Moreover, the sulfoaluminate mortar showed the smallest free shrinkage and the highest restrained shrinkage, mainly due to its high dynamic modulus of elasticity. The pore size distribution of geopolymeric mortar was responsible for the lowest capillary water absorption at short times and for the highest permeability to water vapor and the greatest resistance to sulfate attack.
Structure and Composition of a Dry Mixed-Conifer Forest in Absence of Contemporary Treatments, Southwest, USA
Authors: Cram Douglas , Saud Pradip , Baker Terrell
Published: September 2017

Dry mixed-conifer forests in the Southwest occupy an important ecological and hydrological role in upper watersheds. In the absence of reoccurring fire and silvicultural treatments over the last 50 years, we quantified forest structure and composition on prevailing north and south aspects of a dry mixed-conifer forest in southcentral New Mexico using mixed models and ordination analysis in preparation for an experiment in ecological restoration. Results indicated overstory and midstory were dominated by Douglas-fir (Pseudotsuga menziesii) and shade tolerant/fire intolerant white fir (Abies concolor) with interspersed mature aspen on north aspects, and Douglas-fir and Southwestern white pine (Pinus strobiformis) on south aspects. Ponderosa pine (Pinus ponderosa), which was historically co-dominant with Douglas-fir on north and south aspects, was subdominant on south aspects and almost entirely absent on north aspects. Regeneration was dominated by white fir saplings and seedlings on north aspects while ponderosa pine was completely absent. South aspect saplings and seedlings were characterized by Douglas-fir and Southwestern white pine, but almost no ponderosa pine. Ordination analysis characterized the effect of aspect on species composition. Understanding contemporary forest structure and composition is important when planning for desired future conditions that are to be achieved through ecological restoration using silvicultural techniques designed to foster resilience.
Modelling Bidispersive Local Thermal Non-Equilibrium Flow
Authors: Franchi Franca , Nibbi Roberta , Straughan Brian
Published: September 2017

In this work, we present a system of equations which describes non-isothermal flow in a bidispersive porous medium under conditions of local thermal non-equilibrium. The porous medium consists of macro pores, and in the solid skeleton are cracks or fissures which give rise to micro pores. The temperatures in the solid skeleton and in the fluids in the macro and micro pores are all allowed to be independent. After presenting the general model, we derive a result of universal stability, which guarantees exponential decay of the solution for all initial data. We further present a concrete example by specializing the model to the problem of thermal convection in a layer heated from below.
Weakening Pin Bone Attachment in Fish Fillets Using High-Intensity Focused Ultrasound
Authors: Skjelvareid Martin , Stormo Svein , Þórarinsdóttir Kristín , Heia Karsten
Published: September 2017

High Intensity Focused Ultrasound (HIFU) can be used for the localized heating of biological tissue through the conversion of sound waves into heat. Although originally developed for human medicine, HIFU may also be used to weaken the attachment of pin bones in fish fillets to enable easier removal of such bones. This was shown in the present study, where a series of experiments were performed on HIFU phantoms and fillets of cod and salmon. In thin objects such as fish fillets, the heat is mainly dissipated at the surfaces. However, bones inside the fillet absorb ultrasound energy more efficiently than the surrounding tissue, resulting in a “self-focusing” heating of the bones. Salmon skin was found to effectively block the ultrasound, resulting in a significantly lower heating effect in fillets with skin. Cod skin partly blocked the ultrasound, but only to a small degree, enabling HIFU treatment through the skin. The treatment of fillets to reduce the pin bone attachment yielded an average reduction in the required pulling force by 50% in cod fillets with skin, with little muscle denaturation, and 72% in skinned fillets, with significant muscle denaturation. Salmon fillets were treated from the muscle side of the fillet to circumvent the need for penetration through skin. The treatment resulted in a 30% reduction in the peak pulling force and 10% reduction in the total pulling work, with a slight denaturation of the fillet surface.
Glycemic Responses, Glycemic Index, and Glycemic Load Values of Some Street Foods Prepared from Plantain (Musa spp., AAB Genome) in Côte d’Ivoire
Authors: Kouamé Camille , Kouassi Nestor , Abodo Jacko , Pereko Kingsley , Casiraghi Maria , N’dri Denis , Amani Georges
Published: September 2017

The glycemic index (GI) and glycemic load (GL) of four culinary preferences including five local street dishes prepared from three varieties of plantain at different maturity stages was determined. The GI was obtained following ISO/FDI 26642:2010 protocol, and the GL was calculated from test foods’ GI, considering the amount of available carbohydrate in the traditional portion size. GI values were 44 for Klaclo (with Ameletiha variety at all black stage), 39 for Aloco (with Agnrin variety at full yellow stage), 39 for Aloco (with Agnrin variety at full yellow with black spots stage); 45 for Chips (with Ameletiha variety at green stage) and 89 for Banane braisée (with Afoto variety at light green stage). GI values were inversely correlated with the total sugar and carbohydrate in foods (p < 0.01), and no relationship existed between the GI values and the amount of protein (p = 0.89). Except for Chips (GL = 12), the GLs of the others foods were high (GL > 20). Contrary to Banane braisée, the consumption of Klaclo, Aloco, and Chips may promote the control of postprandial glucose response. Data provides the first GI published values of plantain-based foods commonly consumed in the urban area of Abidjan (Côte d’Ivoire).
Upper Limits to Magnetic Fields in the Outskirts of Galaxies
Authors: López Ericson , Armijos-Abendaño Jairo , Llerena Mario , Aldás Franklin
Published: September 2017

Based on CO(2-1) public data, we study the monoxide oxygen gas excitation conditions and the magnetic field strength of four spiral galaxies. For the galaxy outskirts, we found kinetic temperatures in the range of ≲35–38 K, CO column densities ≲ 10 15 – 10 16 cm - 2 , and H 2 masses ≲ 4 × 10 6 – 6 × 10 8 M ⊙ . An H 2 density ≲ 10 3 cm - 3 is suitable to explain the 2 σ upper limits of the CO(2-1) line intensity. We constrain the magnetic field strength for our sample of spiral galaxies and their outskirts by using their masses and H 2 densities to evaluate a simplified magneto-hydrodynamic equation. Our estimations provide values for the magnetic field strength on the order of ≲6–31 μ G.
Salt-Stress Response Mechanisms Using de Novo Transcriptome Sequencing of Salt-Tolerant and Sensitive Corchorus spp. Genotypes
Authors: Yang Zemao , Lu Ruike , Dai Zhigang , Yan An , Tang Qing , Cheng Chaohua , Xu Ying , Yang Wenting , Su Jianguang
Published: September 2017

High salinity is a major environmental stressor for crops. To understand the regulatory mechanisms underlying salt tolerance, we conducted a comparative transcriptome analysis between salt-tolerant and salt-sensitive jute (Corchorus spp.) genotypes in leaf and root tissues under salt stress and control conditions. In total, 68,961 unigenes were identified. Additionally, 11,100 unigenes (including 385 transcription factors (TFs)) exhibited significant differential expression in salt-tolerant or salt-sensitive genotypes. Numerous common and unique differentially expressed unigenes (DEGs) between the two genotypes were discovered. Fewer DEGs were observed in salt-tolerant jute genotypes whether in root or leaf tissues. These DEGs were involved in various pathways, such as ABA signaling, amino acid metabolism, etc. Among the enriched pathways, plant hormone signal transduction (ko04075) and cysteine/methionine metabolism (ko00270) were the most notable. Eight common DEGs across both tissues and genotypes with similar expression profiles were part of the PYL-ABA-PP2C (pyrabactin resistant-like/regulatory components of ABA receptors-abscisic acid-protein phosphatase 2C). The methionine metabolism pathway was only enriched in salt-tolerant jute root tissue. Twenty-three DEGs were involved in methionine metabolism. Overall, numerous common and unique salt-stress response DEGs and pathways between salt-tolerant and salt-sensitive jute have been discovered, which will provide valuable information regarding salt-stress response mechanisms and help improve salt-resistance molecular breeding in jute.
Comparison of Flood Frequency Analysis Methods for Ungauged Catchments in France
Authors: Odry Jean , Arnaud Patrick
Published: September 2017

The objective of flood frequency analysis (FFA) is to associate flood intensity with a probability of exceedance. Many methods are currently employed for this, ranging from statistical distribution fitting to simulation approaches. In many cases the site of interest is actually ungauged, and a regionalisation scheme has to be associated with the FFA method, leading to a multiplication of the number of possible methods available. This paper presents the results of a wide-range comparison of FFA methods from statistical and simulation families associated with different regionalisation schemes based on regression, or spatial or physical proximity. The methods are applied to a set of 1535 French catchments, and a k-fold cross-validation procedure is used to consider the ungauged configuration. The results suggest that FFA from the statistical family largely relies on the regionalisation step, whereas the simulation-based method is more stable regarding regionalisation. This conclusion emphasises the difficulty of the regionalisation process. The results are also contrasted depending on the type of climate: the Mediterranean catchments tend to aggravate the differences between the methods.
Quantitative Examination of Piezoelectric/Seismoelectric Anomalies from Near-Surface Targets
Authors: Eppelbaum Lev
Published: September 2017

The piezoelectric and seismo-electrokinetic phenomena are manifested by electrical and electromagnetic processes that occur in rocks under the influence of elastic oscillations triggered by shots or mechanical impacts. Differences in piezoelectric properties between the studied targets and host media determine the possibilities of the piezoelectric/seismoelectric method application. Over a long time, an interpretation of obtained data is carried out by the use of methods developed in seismic prospecting. Examination of nature of piezoelectric/seismoelectric anomalies observed in subsurface indicates that these may be related (mainly) to electric potential field. In this paper, it is shown that quantitative analysis of piezoelectric/seismoelectric anomalies may be performed by the advanced and reliable methodologies developed in magnetic prospecting. Some examples from mining geophysics (Russia) and ancient metallurgical site (Israel) confirm applicability of the suggested approach.
“Found Performance”: Towards a Musical Methodology for Exploring the Aesthetics of Care
Authors: Wood Stuart
Published: September 2017

Concepts of performance in fine art reflect key processes in music therapy. Music therapy enables practitioners to reframe patients as performers, producing new meanings around the clinical knowledge attached to medical histories and constructs. In this paper, music therapy practices are considered in the wider context of art history, with reference to allied theories from social research. Tracing a century in art that has revised the performativity of found objects (starting with Duchamp’s “Fountain”), and of found sound (crystallised by Cage’s 4′ 33) this paper proposes that music therapy might be a pioneer methodology of “found performance”. Examples from music therapy and contemporary socially engaged art practices are brought as potential links between artistic methodologies and medical humanities research, with specific reference to notions of Aesthetics of Care.
Relationships of Sexual Dysfunction with Depression and Acceptance of Illness in Women and Men with Type 2 Diabetes Mellitus
Authors: Bąk Ewelina , Marcisz Czeslaw , Krzemińska Sylwia , Dobrzyn-Matusiak Dorota , Foltyn Agnieszka , Drosdzol-Cop Agnieszka
Published: September 2017

An increased prevalence of sexual disorders has been reported in patients with type 2 diabetes. The aim of this study is the assessment of the influence of the psychical condition, the concentration of glycated hemoglobin, the duration of diabetes, the body mass index, the age, and the subjective acceptance of the illness on sexual disorders occurring in women and men with type 2 diabetes. The study enrolled 215 patients (114 women and 101 men) with type 2 diabetes and 183 controls. Sexuality was determined in all of the studied subjects using: the Female Sexual Function Index (FSFI) in women and the International Index of Erectile Function (IIEF) in men. The occurrence of depression symptoms was determined using the Beck Depression Inventory (BDI), whereas the acceptance of the illness in diabetic patients using the Acceptance of Illness Scale (AIS). A sexual dysfunction was found in 68% of the studied diabetic women and 17% of controls. The point values of all the examined FSFI domains were significantly lower in women with diabetes than in controls (p < 0.001). Erectile disorders occurred in 82% of the studied men with diabetes and in 41% of the controls (p < 0.001). The point values of all the domains of FSFI and IIEF demonstrated a significantly negative correlation with the total BDI score, which was higher in patients with diabetes than in patients without diabetes, and a positive correlation with the total AIS score (p < 0.001). The occurrence of sexual dysfunction in patients with diabetes correlated with the age and the duration of diabetes. We conclude that sexual disorders in patients with type 2 diabetes demonstrate the correlation with the occurrence of depression and the acceptance of their illness. Sexual disorders in diabetic patients occur more frequently in older patients and in those with a longer duration of diabetes.
Stress Exposure and Physical, Mental, and Behavioral Health among American Indian Adults with Type 2 Diabetes
Authors: Walls Melissa , Sittner Kelley , Aronson Benjamin , Forsberg Angie , Whitbeck Les , Al’Absi Mustafa
Published: September 2017

American Indian (AI) communities experience disproportionate exposure to stressors and health inequities including type 2 diabetes. Yet, we know little about the role of psychosocial stressors for AI diabetes-related health outcomes. We investigated associations between a range of stressors and psychological, behavioral, and physical health for AIs with diabetes. This community-based participatory research with 5 AI tribes includes 192 AI adult type 2 diabetes patients recruited from clinical records at tribal clinics. Data are from computer-assisted interviews and medical charts. We found consistent bivariate relationships between chronic to discrete stressors and mental and behavioral health outcomes; several remained even after accounting for participant age, gender, and income. Fewer stressors were linked to physical health. We also document a dose–response relationship between stress accumulation and worse health. Findings underscore the importance of considering a broad range of stressors for comprehensive assessment of stress burden and diabetes. Policies and practices aimed at reducing stress exposure and promoting tools for stress management may be mechanisms for optimal health for AI diabetes patients.
Mothers’ Perceived Neighbourhood Environment and Outdoor Play of 2- to 3.5-Year-Old Children: Findings from the Healthy Beginnings Trial
Authors: Xu Huilan , Wen Li , Hardy Louise , Rissel Chris
Published: September 2017

Background: This study aims to investigate whether mothers’ perceived neighbourhood environment is associated with outdoor playtime of 2- to 3.5-year-old children. Methods: Cross-sectional analyses were conducted using data from the Healthy Beginnings Trial (HBT). Data on children’s outdoor playtime and mothers’ perceived neighbourhood environment were collected through face-to-face interviews with mothers when their children were 2 and 3.5 years old. Walk score was obtained from a publicly available website and population density data were obtained from Australian Census data. Multiple logistic regression models were built to investigate these associations. Results: A total of 497 and 415 mother-child dyads were retained at 2 years and 3.5 years. After adjusting for intervention group allocation and other confounding factors, at 2 years, mothers’ perceptions that ‘the neighbourhood is a good place to bring up children’, ‘it is safe to play outside during the day’, and ‘there are good parks or playgrounds in neighbourhood’ were positively associated with children’s outdoor playtime. At 3.5 years, living in a free-standing house was associated with more children’s outdoor playtime. Conclusions: Children may benefit from living in a neighbourhood that supports active lifestyle. Improving social and physical environments in neighbourhoods could be an important strategy for improving young children’s physical activity.
Association between Emotional Symptoms and Job Demands in an Asian Electronics Factory
Authors: Huang Wei-Lieh , Guo Yue Leon , Chen Pau-Chung , Wang Jui , Chu Po-Ching
Published: September 2017

Various work-related issues including mental health have been described for the electronic industry. Although East Asian countries play important roles in the electronics industry, the association between job demands and emotional symptoms has been rarely examined. The present study recruited 603 workers from either office or clean room environments in an electronics factory in Taiwan. Their personal factors, work-related factors, and emotional symptoms were assessed by a self-administered questionnaire. The symptoms of depression and hostility were reported in 24.88% and 24.38% of the subjects, respectively, while 14.93% reported both. A multivariate analysis showed that, overall, women workers were more likely to have emotional symptoms than male workers (odds ration (OR) = 1.50, 95% CI = 1.02–2.18). Among clean room workers, working under high pressure (OR = 1.84, 95% CI = 1.05–3.21), conflicting demands (OR = 2.15, 95% CI = 1.30–3.57), and social isolation at work (OR = 2.99, 95% CI = 1.23–7.30) were associated with emotional symptoms. The findings suggest that in the Asian electronics industry, for women, working under high pressure, conflicting demands, and social isolation at work are risk factors for emotional symptoms, especially for clean room workers. Further large-scale, longitudinal studies are necessary to confirm and prevent the mental health problems in this fast-evolving, highly competitive industry.
In Vitro Glucuronidation of Wushanicaritin by Liver Microsomes, Intestine Microsomes and Expressed Human UDP-Glucuronosyltransferase Enzymes
Authors: Hong Xiaodan , Zheng Yuanru , Qin Zifei , Wu Baojian , Dai Yi , Gao Hao , Yao Zhihong , Gonzalez Frank , Yao Xinsheng
Published: September 2017

Wushanicaritin, a natural polyphenol compound, exerts many biological activities. This study aimed to characterize wushanicaritin glucuronidation by pooled human liver microsomes (HLM), human intestine microsomes and individual uridine diphosphate-glucuronosyltransferase (UGT) enzyme. Glucuronidation rates were determined by incubating wushanicaritin with uridine diphosphoglucuronic acid-supplemented microsomes. Kinetic parameters were derived by appropriate model fitting. Reaction phenotyping, the relative activity factor (RAF) and activity correlation analysis were performed to identify the main UGT isoforms. Wushanicaritin glucuronidation in HLM was efficient with a high CLint (intrinsic clearance) value of 1.25 and 0.69 mL/min/mg for G1 and G2, respectively. UGT1A1 and 1A7 showed the highest activities with the intrinsic clearance (CLint) values of 1.16 and 0.38 mL/min/mg for G1 and G2, respectively. In addition, G1 was significantly correlated with β-estradiol glucuronidation (r = 0.847; p = 0.0005), while G2 was also correlated with chenodeoxycholic acid glucuronidation (r = 0.638, p = 0.026) in a bank of individual HLMs (n = 12). Based on the RAF approach, UGT1A1 contributed 51.2% for G1, and UGT1A3 contributed 26.0% for G2 in HLM. Moreover, glucuronidation of wushanicaritin by liver microsomes showed marked species difference. Taken together, UGT1A1, 1A3, 1A7, 1A8, 1A9 and 2B7 were identified as the main UGT contributors responsible for wushanicaritin glucuronidation.
Why Ubiquitin Has Not Evolved
Authors: Allan Douglas , Phillips James
Published: September 2017

Ubiquitin, discovered less than 50 years ago, tags thousands of diseased proteins for destruction. It is small (only 76 amino acids), and is found unchanged in mammals, birds, fish, and even worms, indicating that ubiquitin is perfect. Key features of its functionality are identified here using critical point thermodynamic scaling theory. These include synchronized pivots and hinges, a stabilizing central pivot, and Fano interference between first- and second-order elements of correlated long-range (allosteric) globular surface shape transitions. Comparison with its closest relative, 76 amino acid Nedd8, shows that the latter lacks all these features. A cracked elastic network model is proposed for the common target shared by many diseased proteins.
Efficacy of Octocog Alfa (Advate) in a Child with Type 3 von Willebrand Disease and Alloantibodies
Authors: Sottilotta Gianluca , Luise Francesca , Massara Elisabetta , Oriana Vincenzo , Piromalli Angela
Published: September 2017

Von Willebrand disease (VWD) is the most frequent inherited bleeding disorder and is caused by either a quantitative and/or qualitative defect of the multimeric glycoprotein vonWillebrand factor (VWF).[...]
Technical Advances in the Measurement of Residual Disease in Acute Myeloid Leukemia
Authors: Roloff Gregory , Lai Catherine , Hourigan Christopher , Dillon Laura
Published: September 2017

Outcomes for those diagnosed with acute myeloid leukemia (AML) remain poor. It has been widely established that persistent residual leukemic burden, often referred to as measurable or minimal residual disease (MRD), after induction therapy or at the time of hematopoietic stem cell transplant (HSCT) is highly predictive for adverse clinical outcomes and can be used to identify patients likely to experience clinically evident relapse. As a result of inherent genetic and molecular heterogeneity in AML, there is no uniform method or protocol for MRD measurement to encompass all cases. Several techniques focusing on identifying recurrent molecular and cytogenetic aberrations or leukemia-associated immunophenotypes have been described, each with their own strengths and weaknesses. Modern technologies enabling the digital quantification and tracking of individual DNA or RNA molecules, next-generation sequencing (NGS) platforms, and high-resolution imaging capabilities are among several new avenues under development to supplement or replace the current standard of flow cytometry. In this review, we outline emerging modalities positioned to enhance MRD detection and discuss factors surrounding their integration into clinical practice.
Wolf and Bear Depredation on Livestock in Northern Sweden 1827–2014: Combining History, Ecology and Interviews
Authors: Axelsson Linkowski Weronika , Kvarnström Marie , Westin Anna , Moen Jon , Östlund Lars
Published: September 2017

During the twenty-first century, large carnivores have increased in human dominated landscapes after being extinct or nearly extinct. This has resulted in increasing numbers of livestock killed by large carnivores. The intent of this paper is to give a land use-historical perspective on the recent livestock–carnivore conflict in boreal Sweden. More specifically we address: (1) depredation risks (livestock killed by carnivores) and (2) local knowledge of how to protect livestock from predation and whether it survived among pastoralists until the present. This study provides numeric information on carnivores, livestock and depredation, combined with oral information from summer farmers about livestock protection. We compare recent (since 1998) and historical (late nineteenth century) depredation rates in two Swedish counties. In Dalarna recent depredation rates are higher than historical rates while the opposite pattern is seen in Jämtland. Recent depredation rates in Dalarna are twice the recent rates in Jämtland, in contrast to the historical situation. Recent and historical depredation rates are of the same order. Summer farmers traditionally graze their livestock in forested areas where carnivores reside. Interviews show that traditional knowledge of how to protect livestock from carnivores was lost during the twentieth century, but recently new knowledge has developed leading to changes in summer farming practices. The carnivore–livestock situation today differs from the historical situation, not so much in levels of depredation, but mainly regarding the possibilities of farmers to face challenges associated with increasing carnivore populations.
Vegetation in Drylands: Effects on Wind Flow and Aeolian Sediment Transport
Authors: Mayaud Jerome , Webb Nicholas
Published: September 2017

Drylands are characterised by patchy vegetation, erodible surfaces and erosive aeolian processes. Empirical and modelling studies have shown that vegetation elements provide drag on the overlying airflow, thus affecting wind velocity profiles and altering erosive dynamics on desert surfaces. However, these dynamics are significantly complicated by a variety of factors, including turbulence, and vegetation porosity and pliability effects. This has resulted in some uncertainty about the effect of vegetation on sediment transport in drylands. Here, we review recent progress in our understanding of the effects of dryland vegetation on wind flow and aeolian sediment transport processes. In particular, wind transport models have played a key role in simplifying aeolian processes in partly vegetated landscapes, but a number of key uncertainties and challenges remain. We identify potential future avenues for research that would help to elucidate the roles of vegetation distribution, geometry and scale in shaping the entrainment, transport and redistribution of wind-blown material at multiple scales. Gaps in our collective knowledge must be addressed through a combination of rigorous field, wind tunnel and modelling experiments.
Precipitation Behavior and Quenching Sensitivity of a Spray Deposited Al-Zn-Mg-Cu-Zr Alloy
Authors: Sheng Xiaofei , Lei Qian , Xiao Zhu , Wang Mingpu
Published: September 2017

Precipitation behavior and the quenching sensitivity of a spray deposited Al-Zn-Mg-Cu-Zr alloy during isothermal heat treatment have been studied systematically. Results demonstrate that both the hardness and the ultimate tensile strength of the studied alloy decreased with the isothermal treatment time at certain temperatures. More notably, the hardness decreases rapidly after the isothermal heat treatment. During isothermal heat treatment processing, precipitates readily nucleated in the medium-temperature zone (250–400 °C), while the precipitation nucleation was scarce in the low-temperature zone (<250 °C) and in the high-temperature zone (>400 °C). Precipitates with sizes of less than ten nanometers would contribute a significant increase in yield strength, while the ones with a larger size than 300 nm would contribute little strengthening effect. Quenching sensitivity is high in the medium-temperature zone (250–400 °C), and corresponding time-temperature-property (TTP) curves of the studied alloy have been established.
Superplastic Deformation Mechanisms of Superfine/Nanocrystalline Duplex PM-TiAl-Based Alloy
Authors: Gong Xuebo , Duan Zhenxin , Pei Wen , Chen Hua
Published: September 2017

In this paper, the equiaxed superfine/nanocrystalline duplex PM-TiAl-based alloy with (γ + α2) microstructure, Ti-45Al-5Nb (at %), has been synthesized by high-energy ball milling and vacuum hot pressing sintering. Superplastic deformation behavior has been investigated at 1000 °C and 1050 °C with strain rates from 5 × 10−5 s−1 to 1 × 10−3 s−1. The effects of deformation on the microstructure and mechanical behaviors of high Nb containing TiAl alloy have been characterized and analyzed. The results showed that, the ultimate tensile strength of the alloy was 58.7 MPa at 1000 °C and 10.5 MPa at 1050 °C with a strain rate of 5 × 10−5 s−1, while the elongation was 121% and 233%, respectively. The alloy exhibited superplastic elongation at 1000 and 1050 °C with an exponent (m) of 0.48 and 0.45. The main softening mechanism was dynamic recrystallization of γ grains; the dislocation slip and γ/γ interface twinning were responsible for superplastic deformation. The orientation relationship of γ/γ interface twinning obeyed the classical one: (001)γ//(110)γ.
The Catastrophe of Electric Vehicle Sales
Authors: Sands Timothy
Published: September 2017

Electric vehicles have undergone a recent faddy trend in the United States and Europe, and several recent publications trumpet the continued rise of electric vehicles citing steadily-climbing monthly vehicle sales. The broad purpose of this study is to examine this optimism with some degree of mathematical rigor. Specifically, the methodology will use catastrophe theory to explore the possibility of a sudden, seemingly-unexplainable crash in vehicle sales. The study begins by defining optimal system equations that well-model the available sales data. Next, these optimal models are used to investigate the potential response to a slow dynamic acting on the relatively faster dynamic of the optimal system equations. Catastrophe theory indicates a potential sudden crash in sales when a slow dynamic is at-work. It is noteworthy that the prediction can be made even while sales are increasing.
Study of the Microstructure Evolution and Properties Response of a Friction-Stir-Welded Copper-Chromium-Zirconium Alloy
Authors: Lai Ruilin , He Diqiu , He Guoai , Lin Junyuan , Sun Youqing
Published: September 2017

In this article, the copper-chromium-zirconium (CuCrZr) alloys plates with 21 mm in thickness were butt joined together by means of FSW (friction stir welding). The properties of the FSW joints are studied. The microstructure variations during the process of FSW were investigated by optical microscopy (OM), electron back-scattered diffraction (EBSD), and transmission electron microscopy (TEM). The results show that the grains size in the nugget zone (NZ) are significantly refined, which can be attributed to the dynamic recrystallization (DRX). The microstructure distribution in the NZ is inhomogeneous and the size of equiaxed grains are decreased gradually along the thickness direction from the top to bottom area of the welds. Meanwhile, it is found that the micro-hardness and tensile strength of the welds are slightly increased along the thickness direction from the top to the bottom area of the welds. All the nano-strengthening precipitates in the BM are dissolved into the Cu matrix in the NZ. Therefore, the decreases in hardness, tensile strength, and electrical conductivity can be attributed to the comprehensive effect of dissolution of nano-strengthening precipitates into the supersaturation matrix and severe DRX in the welded NZ.
Proteomic Characterization of Armillaria mellea Reveals Oxidative Stress Response Mechanisms and Altered Secondary Metabolism Profiles
Authors: Collins Cassandra , Hurley Rachel , Almutlaqah Nada , O’Keeffe Grainne , Keane Thomas , Fitzpatrick David , Owens Rebecca
Published: September 2017

Armillaria mellea is a major plant pathogen. Yet, the strategies the organism uses to infect susceptible species, degrade lignocellulose and other plant material and protect itself against plant defences and its own glycodegradative arsenal are largely unknown. Here, we use a combination of gel and MS-based proteomics to profile A. mellea under conditions of oxidative stress and changes in growth matrix. 2-DE and LC-MS/MS were used to investigate the response of A. mellea to H2O2 and menadione/FeCl3 exposure, respectively. Several proteins were detected with altered abundance in response to H2O2, but not menadione/FeCl3 (i.e., valosin-containing protein), indicating distinct responses to these different forms of oxidative stress. One protein, cobalamin-independent methionine synthase, demonstrated a common response in both conditions, which may be a marker for a more general stress response mechanism. Further changes to the A. mellea proteome were investigated using MS-based proteomics, which identified changes to putative secondary metabolism (SM) enzymes upon growth in agar compared to liquid cultures. Metabolomic analyses revealed distinct profiles, highlighting the effect of growth matrix on SM production. This establishes robust methods by which to utilize comparative proteomics to characterize this important phytopathogen.
CO2 Absorption and Magnesium Carbonate Precipitation in MgCl2–NH3–NH4Cl Solutions: Implications for Carbon Capture and Storage
Authors: Zhu Chen , Wang Han , Li Gen , An Siyu , Ding Xiaofeng , Teng Hui , Zhao Liang
Published: September 2017

CO2 absorption and carbonate precipitation are the two core processes controlling the reaction rate and path of CO2 mineral sequestration. Whereas previous studies have focused on testing reactive crystallization and precipitation kinetics, much less attention has been paid to absorption, the key process determining the removal efficiency of CO2. In this study, adopting a novel wetted wall column reactor, we systematically explore the rates and mechanisms of carbon transformation from CO2 gas to carbonates in MgCl2–NH3–NH4Cl solutions. We find that reactive diffusion in liquid film of the wetted wall column is the rate-limiting step of CO2 absorption when proceeding chiefly through interactions between CO2(aq) and NH3(aq). We further quantified the reaction kinetic constant of the CO2–NH3 reaction. Our results indicate that higher initial concentration of NH4Cl ( ≥ 2 mol · L − 1 ) leads to the precipitation of roguinite [ ( NH 4 ) 2 Mg ( CO 3 ) 2 · 4 H 2 O ], while nesquehonite appears to be the dominant Mg-carbonate without NH4Cl addition. We also noticed dypingite formation via phase transformation in hot water. This study provides new insight into the reaction kinetics of CO2 mineral carbonation that indicates the potential of this technique for future application to industrial-scale CO2 sequestration.
Indium Mineralization in the Xianghualing Sn-Polymetallic Orefield in Southern Hunan, Southern China
Authors: Liu Jianping , Rong Yanan , Zhang Shugen , Liu Zhongfa , Chen Weikang
Published: September 2017

Although numerous W–Sn–Pb–Zn polymetallic deposits are located in southern Hunan, and In-bearing deposits are related to W–Sn–Pb–Zn polymetallic deposits, Indium mineralization in southern Hunan is poorly studied. In order to investigate the In mineralization of the Xianghualing orefield, which is a typical orefield in southern Hunan, ore bulk chemistry, microscopic observation, and electron-probe microanalysis of vein-type (type-I) and porphyry-type (type-II) Sn–Pb–Zn orebodies were studied. The In contents of the type-I orebodies varies from 0.79 to 1680 ppm (avg. 217 ppm, n = 29), and that of the type-II orebodies varies from 10 to 150 ppm (avg. 64 ppm, n = 10). Although chalcopyrite and stannite contain trace amounts of In, sphalerite is the most important In-rich mineral in the orefield. Sphalerite in type-I orebodies contains from <0.02 to 21.96 wt % In, and in type-II orebodies contains from <0.02 to 0.39 wt % In. Indium-rich chemical-zoned sphalerite contains 7 to 8 wt % In in its core and up to 21.96 wt % In in its rim. This sphalerite may be the highest In-bearing variety in Southern China. The Cd contents of the In-rich sphalerite ranges from 0.35 to 0.45 wt %, which places it in the the “Indium window” of the Cu–In–S phases. The geological and structural features of the Xianghualing orefield indicate that the In mineralization of the two types of In-bearing Sn–Pb–Zn orebodies is related to the volatile-rich, In-rich, A-type granites, and is controlled by the normal faults of magmatic-diapiric activity extensional features.
Transesterification Synthesis of Chloramphenicol Esters with the Lipase from Bacillus amyloliquefaciens
Authors: Dong Fengying , Li Lingmeng , Lin Lin , He Dannong , Chen Jingwen , Wei Wei , Wei Dongzhi
Published: September 2017

This work presents a synthetic route to produce chloramphenicol esters by taking advantage the high enantio- and regio-selectivity of lipases. A series of chloramphenicol esters were synthesized using chloramphenicol, acyl donors of different carbon chain length and lipase LipBA (lipase cloned from Bacillus amyloliquefaciens). Among acyl donors with different carbon chain lengths, vinyl propionate was found to be the best. The influences of different organic solvents, reaction temperature, reaction time, enzyme loading and water content on the synthesis of the chloramphenicol esters were studied. The synthesis of chloramphenicol propionate (0.25 M) with 4.0 g L−1 of LipBA loading gave a conversion of ~98% and a purity of ~99% within 8 h at 50 °C in 1,4-dioxane as solvent. The optimum mole ratio of vinyl propionate to chloramphenicol was increased to 5:1. This is the first report of B. amyloliquefaciens lipase being used in chloramphenicol ester synthesis and a detailed study of the synthesis of chloramphenicol propionate using this reaction. The high enzyme activity and selectivity make lipase LipBA an attractive catalyst for green chemical synthesis of molecules with complex structures.
Antibiofilm Activity and Mechanism of Action of the Disinfectant Chloramine T on Candida spp., and Its Toxicity against Human Cells
Authors: Ferreira Gabriela , Rosalen Pedro , Peixoto Larissa , Pérez Ana , Carlo Fabíola , Castellano Lúcio , Lima Jefferson , Freires Irlan , Lima Edeltrudes , Castro Ricardo
Published: September 2017

We evaluated the antifungal and anti-biofilm activity, mechanism of action and cytotoxicity of chloramine T trihydrate (CAT) against Candida spp. The Minimum Inhibitory and Fungicidal Concentrations (MIC/MFC) of CAT were determined. Changes in CAT-treated C. albicans growth kinetics and micromorphology were evaluated, as well as the mechanism of action, and its effects on biofilm. Cytotoxicity was assessed by the hemolysis method. The data were analyzed by inferential statistics (p ≤ 0.05). CAT showed antifungal activity against all strains, with MIC values ranging between 1.38 and 5.54 mmol/L (MIC75%: 2.77 mmol/L). CAT demonstrated an immediate and sustained action on C. albicans growth kinetics, particularly at 2 × MIC. This compound likely acts on the cell wall and membrane permeability simultaneously and was found to cause changes in C. albicans micromorphology. Tha antibiofilm activity of CAT was similar to that of sodium hypochlorite (p > 0.05) against mature biofilms. CAT was more effective than NaOCl in reducing mature biofilm upon 1-min exposure at 2 × MIC (24 h) and 4 × MIC (48 h) (p < 0.05). Toxicological analysis revealed that CAT had hemolytic activity between 61 and 67.7% as compared to 100% by NaOCl. CAT has antifungal and anti-biofilm properties, probably acting on both cell wall and membrane permeability, and showed low toxicity in vitro.
The Influence of Accelerated UV-A and Q-SUN Irradiation on the Antimicrobial Properties of Coatings Containing ZnO Nanoparticles
Authors: Mizielińska Małgorzata , Łopusiewicz Łukasz , Mężyńska Monika , Bartkowiak Artur
Published: September 2017

The influence of accelerated UV-A and Q-SUN irradiation on the antimicrobial properties of coatings containing ZnO nanoparticles was investigated using a polyethylene (PE) film covering. The results of the study showed that Methyl Hydroxypropyl Celluloses (MHPC) coatings did not influence the growth of S. aureus, B. cereus, E. coli, P. aeruginosa or C. albicans cells. MHPC coatings containing ZnO nanoparticles inhibited the growth of bacterial strains and reduced the number of C. albicans strains. Accelerated Q-SUN and UV-A irradiation had no influence on the antimicrobial effect of nano ZnO coatings against S. aureus, B. cereus and E. coli. Q-SUN irradiation decreased the activity of MHPC coatings containing nanoparticles against P. aeruginosa and C. albicans. An FT-IR analysis clearly showed that ZnO nanoparticles shielded the MHPC coating during Q-SUN irradiation.