bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Deep Straw Return under Saline Conditions on Soil Nutrient and Maize Growth in Saline–Alkali Land
Authors: Zhang Hao , Gao Julin , Yu Xiaofang , Ma Daling , Hu Shuping , Shen Tianao
Published: February 2023

To clarify the effect of tillage methods on saline–alkali land improvement and maize growth in cropland salinized to different degrees, we set up two treatments (shallow rotation (15 cm depth; CK) and deep straw return (35 cm depth; DPR)) in land characterized by three different salinization degrees and analyzed the effects of the two treatments on soil nutrient content, salinity index, chlorophyll fluorescence, growth status, and yield at three salinization levels. The results show that (1) compared with CK, alkaline N, total N, Olsen P, exchangeable K, and organic matter in saline soils were all significantly improved, and total salt and pH values were reduced by 34.01–50.79% and 2.56–7.54%, respectively, under deep straw return conditions, representing the largest values in moderately saline–alkali land. (2) Compared with CK, chlorophyll fluorescence was significantly improved, and maximum photochemical efficiency (Fv/Fm), photochemical quenching (qP), and effective quantum yield of PSII (ΦPSII) were significantly increased by 8.09–15.41%, 9.13–17.93%, and 38.79–70.83% following deep straw return treatment; these increases were the largest ones observed in moderately saline–alkali land. (3) Deep straw return promoted the growth of maize and significantly increased the yield of maize. Plant height, leaf area index, and yield increased the most in moderately saline–alkali land and increased by 6.84–21.79%, 0.59–2.28 units, and 12.78–28.07%, respectively. The yield increased by 33.89 %, which was mainly due to the increase in 1000-grain weight. The results provide a theoretical basis for taking straw return measures to improve soil and increase maize yield in saline–alkali land.
The Physicochemical and Nutritional Value of Fresh and Processed Portulacaria afra (Spekboom) Leaves
Authors: Mahlanza Zamancwane P. , de Wit Maryna , Hugo Arnold , du Toit Alba
Published: February 2023

Wild-growing edible flora should be investigated to improve human food sustainability and security. Portulacaria afra (spekboom) is an undervalued edible succulent plant that thrives in harsh conditions in Southern Africa. This study determined whether the indigenous edible spekboom can be harnessed as a sustainable, nutritious food source and ingredient. Physicochemical and nutritional analyses were conducted on fresh and processed leaves (preserves, chutney, pickles, and spice). The physicochemical characteristics included morphology, color, texture, °Brix, pH, water activity, moisture content, titratable acidity, and ascorbic acid. Nutritional value determinants included crude protein, crude fiber, gross energy, and total fat content. The small, dark green fresh leaves had a soft texture and a very low sugar and fat content. Although the water content was high, water activity was low. The pH and titratable acidity showed high acidity. Leaves were high in ascorbic acid and low in protein, energy, insoluble fiber, and total fats. The leaves were successfully processed into preserved products providing possible applications in the food industry other than fresh leaves. The high acidity could be researched for application as a functional ingredient. This study provided basic information on an unexplored plant that may have potential in the food industry.
Comparative Transcriptome Analysis Identified Potential Genes and Transcription Factors for Flower Coloration in Kenaf (Hibiscus cannabinus L.)
Authors: Lyu Jae Il , Ryu Jaihyunk , Kim Dong-Gun , Kim Jung Min , Ahn Joon-Woo , Kwon Soon-Jae , Kim Sang Hoon , Kang Si-Yong
Published: February 2023

The biochemical compounds in kenaf leaves and flowers mainly consist of flavonoids, including flavonoid glycosides and floral anthocyanins. In the present study, we performed comparative transcriptome analysis using RNA-sequencing and identified putative genes involved in flower coloration in different flower developmental stages of three kenaf mutants including Baekma (white flower), Jangdae (ivory flower), and Bora (purple flower). A total of 36.1 Gb reads were generated for two kenaf accessions and 38,601 representative transcripts with an average length of 1350 bp were yielded, of which 33,057 (85.64%) were annotated against two databases. Expression profiling of the transcripts identified 1044 and 472 differentially expressed genes (DEGs) among three mutants in the young bud and full bloom stages, respectively. KEGG enrichment analysis of these DEGs revealed that the representative pathway was “biosynthesis of secondary metabolites”, including phenylpropanoid biosynthesis and flavonoid biosynthesis. Consequently, we investigated genes related to the phenylpropanoid pathway, which included 45 DEGs from a total of 1358. Our results provide useful information for understanding gene functions of flower coloration in kenaf, which will be useful in further studies.
Salinity Properties Retrieval from Sentinel-2 Satellite Data and Machine Learning Algorithms
Authors: Mzid Nada , Boussadia Olfa , Albrizio Rossella , Stellacci Anna Maria , Braham Mohamed , Todorovic Mladen
Published: February 2023

The accurate monitoring of soil salinization plays a key role in the ecological security and sustainable agricultural development of semiarid regions. The objective of this study was to achieve the best estimation of electrical conductivity variables from salt-affected soils in a south Mediterranean region using Sentinel-2 multispectral imagery. In order to realize this goal, a test was carried out using electrical conductivity (EC) data collected in central Tunisia. Soil electrical conductivity and leaf electrical conductivity were measured in an olive orchard over two growing seasons and under three irrigation treatments. Firstly, selected spectral salinity, chlorophyll, water, and vegetation indices were tested over the experimental area to estimate both soil and leaf EC using Sentinel-2 imagery on the Google Earth Engine platform. Subsequently, estimation models of soil and leaf EC were calibrated by employing machine learning (ML) techniques using 12 spectral bands of Sentinel-2 images. The prediction accuracy of the EC estimation was assessed by using k-fold cross-validation and computing statistical metrics. The results of the study revealed that machine learning algorithms, together with multispectral data, could advance the mapping and monitoring of soil and leaf electrical conductivity.
Estimating Soil Hydraulic Parameters during Ponding Infiltration Using a Hybrid Algorithm
Authors: Li Yibo , Liu Ye , Ma Xiaoyi
Published: February 2023

Accurate inversion of soil hydraulic parameters based on the van Genuchten–Mualem model has received much attention in soil science research. Herein, a hybrid algorithm method using particle swarm optimization and vector-evaluated genetic algorithm was used to invert the parameters θs, α, n, and Ks, with the objective functions of infiltration rate, cumulative infiltration, and soil water content. Then, numerical experiments were conducted on four typical soils at three initial water content levels (20, 40, and 60% effective saturation) to verify the accuracy of the inverse method. The results showed that the inversed soil water retention and conductivity curves were approximately the same as the real curves, with the root mean square errors of 0.00101–0.00192 cm3·cm−3, 0.00800–0.02519 cm3·cm−3, respectively, and both the Nash-Sutcliffe coefficients were approximately 1.0. Additionally, laboratory experiments were also performed to compare with the inversed parameters for verification, within small root mean squared errors and approximately 1.0 Nash–Sutcliffe coefficients. Furthermore, the method can also achieve acceptably accurate parameter inversion even with substantial measurement errors included in the cumulative infiltration, initial water content, and final water content. Thus, the method is effective and robust and found to be practical in field experiments.
Beef Nutritional Characteristics, Fat Profile and Blood Metabolic Markers from Purebred Wagyu, Crossbred Wagyu and Crossbred European Steers Raised on a Fattening Farm in Spain
Authors: Vázquez-Mosquera Juan M. , Fernandez-Novo Aitor , de Mercado Eduardo , Vázquez-Gómez Marta , Gardon Juan C. , Pesántez-Pacheco José L. , Revilla-Ruiz Ángel , Patrón-Collantes Raquel , Pérez-Solana Maria L. , Villagrá Arantxa , Martínez Daniel , Sebastián Francisco , Pérez-Garnelo Sonia S. , Astiz Susana
Published: February 2023

A high intramuscular fat content characterizes Wagyu (WY) cattle breed. Our objective was to compare beef from WY, WY-by-Angus, or Wangus (WN) steers with European, Angus-by-Charolais-Limousine crossbred steers (ACL), considering metabolic biomarkers pre-slaughtering and nutritional characteristics, including health-related indexes of the lipid fraction. The fattening system with olein-rich diets and no exercise restriction included 82 steers, 24 WY, 29 WN, and 29 ACL. The slaughter ages and weights were (median and interquartile range) 38.4 mo.-old (34.9–40.3 mo.) and 840 kg (785–895 kg) for WY; for WN, 30.6 mo. (26.9–36.5 mo.) and 832 kg (802–875 kg), and for ACL steers, 20.3 mo.-old (19.0–22.7 mo.) and 780 kg (715–852 kg). Blood lipid-related metabolites, except for non-esterified fatty acids (NEFA) and low-density level cholesterol (LDL), were higher in WY and WN than in ACL, while glucose was lower in WY and WN. Leptin was higher in WN than in ACL. Pre-slaughtering values of plasma HDL underscored as a possible metabolic biomarker directly related to beef quality. The amino-acid content in beef did not differ among experimental groups, except for more crude protein in ACL. Compared to ACL, WY steers showed higher intramuscular fat in sirloin (51.5 vs. 21.9%) and entrecote (59.6 vs. 27.6%), more unsaturated fatty acids in entrecote (55.8 vs. 53.0%), and more oleic acid in sirloin (46 vs. 41.3%) and entrecote (47.5 vs. 43.3%). Compared to ACL entrecote, WY and WN showed better atherogenic (0.6 and 0.55 vs. 0.69), thrombogenicity (0.82 and 0.92 vs. 1.1), and hypocholesterolemic/hypercholesterolemic index (1.9 and 2.1 vs. 1.7). Therefore, beef’s nutritional characteristics depend on breed/crossbred, slaughtering age and cut, with WY and WN entrecote samples showing a healthier lipid fraction.
Effects of the Replacement of Dietary Fish Meal with Poultry By-Product Meal on Growth and Intestinal Health of Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
Authors: Qiu Zongsheng , Xu Qiyou , Xie Dazhang , Zhao Jiantao , Yamamoto Fernando Y. , Xu Hong , Zhao Jianhua
Published: February 2023

To investigate the effect of poultry by-product meal (PBM) replacing fish meal on the growth and intestinal health of Chinese soft-shelled turtle (Pelodiscus sinensis). Four experimental diets were prepared. Fish meal was replaced by 0 (control group, PBM0), 5% (PBM5), 10% (PBM10), and 15% (PBM15) PBM. Compared to the control group, final body weight, weight gain, and specific growth rate were significantly increased, while feed conversion rate decreased significantly in the PBM10 group (p < 0.05). The PBM15 group significantly increased the moisture content and significantly decreased the ash content of the turtles (p < 0.05). The PBM5 and PBM15 groups significantly decreased the whole-body crude lipid (p < 0.05). The serum glucose content increased significantly in the PBM10 group (p < 0.05). The liver malonaldehyde content significantly decreased in the PBM5 group and in the PBM10 group (p < 0.05). Liver glutamic-oxalacetic transaminase and intestinal pepsin activity were increased significantly in the PBM15 group (p < 0.05). The expression of the intestinal interleukin 10 (IL-10) gene was significantly down-regulated in the PBM10 group and the PBM15 group (p < 0.05), the expression of the intestinal interferon-γ (IFN-γ), interleukin-8 (IL-8), and liver toll-like receptor 4 (TLR4) and toll-like receptor 5 (TLR5) genes were significantly up-regulated in the PBM5 group (p < 0.05). In summary, poultry by-product meal can be used as a protein source to replace fish meal in turtle feed. Based on quadratic regression analysis, the optimal replacement ratio is 7.39%.
Extracts of Apricot (Prunus armeniaca) and Peach (Prunus pérsica) Kernels as Feed Additives: Nutrient Digestibility, Growth Performance, and Immunological Status of Growing Rabbits
Authors: Basyony Mohamed , Morsy Amr S. , Soltan Yosra A.
Published: February 2023

This study assessed the effects of the kernel extracts of apricot (AKE; Prunus armeniaca) and peach (PKE; Prunus pérsica), and their mixture (Mix) on growth efficiency, feed utilization, cecum activity, and health status, of growing rabbits. Weaned male New Zealand White rabbits at six weeks old [n = 84, 736 ± 24 SE g body weight (BW)] were randomly allotted to four dietary groups. The first group received no feed additives (control), the second and third groups received 0.3 mL/kg BW of AKE and PKE, respectively, and the fourth group received a mixture of AKE and PKE (1:1) at 0.3 mL/kg BW (Mix). Results indicated that 2(3h)-Furanone, 5-Heptyldihydro was found in abundance in both extracts, while 1,1-Dimethyl-2 Phenylethy L Butyrate and 1,3-Dioxolane, and 4-Methyl-2-Phenyl- were the most components detected in AKE and Cyclohexanol and 10-Methylundecan-4-olide were found in abundance in PKE. All the experimental extracts enhanced (p < 0.05) the growth performance, cecal fermentation parameters, and cecal L. acidiophilus and L. cellobiosus count, while PKE and the mixture treatments presented the highest (p = 0.001) total weight gain and average weight gain without affecting the feed intake. Rabbits that received the mix treatment had the highest (p < 0.05) nutrient digestibility and nitrogen retained, and the lowest (p = 0.001) cecal ammonia concentration. All the experimental extracts enhanced (p < 0.05) the blood antioxidant indicators (including total antioxidant capacity, catalase, and superoxide dismutase concentrations), and immune response of growing rabbits. In general, fruit kernel extracts are rich sources of bioactive substances that can be used as promising feed additives to promote the growth and health status of weaned rabbits.
Survived the Glaciations, Will They Survive the Fish? Allochthonous Ichthyofauna and Alpine Endemic Newts: A Road Map for a Conservation Strategy
Authors: Bernabò Ilaria , Iannella Mattia , Cittadino Viviana , Corapi Anna , Romano Antonio , Andreone Franco , Biondi Maurizio , Gallo Splendore Marcellino , Tripepi Sandro
Published: February 2023

The Calabrian Alpine newt (Ichthyosaura alpestris inexpectata) is a glacial relict with small and extremely localised populations in the Catena Costiera (Calabria, Southern Italy) and is considered to be “Endangered” by the Italian IUCN assessment. Climate-induced habitat loss and recent fish introductions in three lakes of the Special Area of Conservation (SAC) Laghi di Fagnano threaten the subspecies’ survival in the core of its restricted range. Considering these challenges, understanding the distribution and abundance of this newt is crucial. We surveyed the spatially clustered wetlands in the SAC and neighbouring areas. First, we provide the updated distribution of this subspecies, highlighting fish-invaded and fishless sites historically known to host Calabrian Alpine newt populations and two new breeding sites that have been recently colonised. Then, we provide a rough estimate of the abundance, body size and body condition of breeding adults and habitat characteristics in fish-invaded and fishless ponds. We did not detect Calabrian Alpine newts at two historically known sites now invaded by fish. Our results indicate a reduction in occupied sites and small-size populations. These observations highlight the need for future strategies, such as fish removal, the creation of alternative breeding habitats and captive breeding, to preserve this endemic taxon.
Social Reward Behaviour in Two Groups of European Grey Wolves (Canis lupus lupus)—A Case Study
Authors: Tebelmann Hana , Gansloßer Udo
Published: February 2023

Prosociality occurs in many species and is likely to be a crucial factor for the survival of group-living animals. Social feedback is an important mechanism for the coordination of group decisions. Since group-living animals with specific personality axes, i.e., boldness, are known to provide certain benefits for their group, bold actions might receive more prosocial feedback than other actions. Our case study aims to determine whether bold behaviour, i.e., novel object interaction (Nobj), might be answered more frequently with prosocial behaviours. We investigated the differences in the frequency of occurrence in prosocial behaviours after three different individual actions in two groups of grey wolves. We aim to outline the development of a social reward behavioural category as part of social feedback mechanisms. We used Markov chain models for probability analyses, and a non-parametric ANOVA to test for differences between the influences of individual behaviours on the probability of a prosocial behaviour chain. We additionally tested for the potential influences of age, sex and personality on the frequency of Nobj. Our results suggest that bold interactions are more often responded to with prosocial behaviour. Bold behaviour might be more often socially rewarded because of its benefits for group-living animals. More research is needed to investigate whether bold behaviour is more frequently responded to prosocially, and to investigate the social reward phenomenon.
Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone
Authors: Bevilaqua Júlia Ribeiro , Rodriguez Mariana Garcia Kako , Maciel Giovanna Serpa , Vergani Gabriel Brun , Fonseca Jeferson Ferreira da , Bartlewski Pawel Mieczyslaw , Oliveira Maria Emilia Franco
Published: February 2023

Premature regression of corpora lutea (PRCL) may adversely affect the outcome of hormonal ovarian superstimulation in small ruminants, and the total dose of exogenous gonadotropins used may be one of the causes of this condition. There were two major objectives of the present study: (1) to evaluate the effects of different superovulatory doses of porcine follicle-stimulating hormone (pFSH) on the biometry, blood perfusion (Doppler), and echotextural characteristics of luteal structures; and, (2) to determine the usefulness of biometric, vascular, and echotextural luteal variables, as well as measurements of circulating progesterone (P4) concentrations for early detection of PRCL in superovulated Santa Inês ewes. Twenty-seven Santa Inês ewes received an intravaginal P4-releasing device (CIDR) from Days 0 to 8 (Day 0 = random day of the anovulatory period). An IM injection of d-cloprostenol (37.5 μg) was given at the time of the CIDR insertion and withdrawal. On Day 6, all the ewes received 300 IU of eCG IM and were divided into three treatment groups (each n = 9): G100 (100 mg); G133 (133 mg); and G200 (200 mg of pFSH) administered IM every 12 h in eight injections. Transrectal ovarian ultrasonography and jugular blood sampling for serum P4 measurements were performed on Days 11 to 15. On the day of embryo recovery (Day 15), all the ewes underwent diagnostic videolaparoscopy and were classified, based on their luteal characteristics, into three response groups: nCL (ewes with normal CL only); rCL (ewes with regressing CL only); and ewes with both nCL and rCL following the superovulatory regimen. Our present results indicate that the total pFSH doses of 100 mg and 200 mg result in similar ovulatory responses and luteal function/biometrics, although the percentage of donor ewes with nCL was greater (p < 0.05) for G100 compared with the G200 animals. An application of 133 mg of pFSH was associated with diminished luteogenesis. Lastly, circulating P4 concentrations, ultrasonographic estimates of total luteal area, and CL pixel heterogeneity (standard deviation of numerical pixel values) are promising markers of luteal inadequacy in superovulated ewes.
Effects of Water Temperature on Gonads Growth in Ambystoma mexicanum Axolotl Salamanders
Authors: Figiel Chester R.
Published: February 2023

The thermal environment is a major factor influencing amphibians. For example, amphibian reproduction occurs in specific temperatures, and minor changes in this aspect could have negative impacts on this biological process. Understanding the potential effects of temperature on reproductive output is important from both an ecological and captive breeding colony point of view. I examined temperature effects on reproduction in axolotl reared from egg to adult at 4 temperatures (15 °C, 19 °C, 23 °C, and 27 °C) These adult axolotls (n = 174) were measured and weighed, dissected, and their gonads were removed and weighed to obtain an individual’s reproductive allocation. Female axolotls reared at 23 °C had a greater Gonadosomatic Index (GSI) compared to axolotl reared at each of the other temperatures with axolotls reared at 27 °C having the lowest reproductive output. Moreover, all GSI pair-wise comparisons in the four temperature treatments were significantly different from each other (ANOVA, F (3, 66) = 61.681, p < 0.0001). Additionally male rearing temperature significantly influenced GSI (ANOVA, F (3, 89) = 10.441, p < 0.0001). Male axolotls reared at 19 °C had significantly greater GSI compared to males reared at the three other temperatures. There were no statistical differences among each of the other pair-wise comparisons. As seen in this experiment, axolotls may be especially sensitive to climate-driven warming due to their highly permeable skin and paedomorphic life history. Understanding how axolotls and other amphibian species adjust to the challenges of climate change is important in the management of this imperiled taxa.
Systematic Identification and Comparison of the Expressed Profiles of Exosomal MiRNAs in Pigs Infected with NADC30-like PRRSV Strain
Authors: Cheng Feng , Wang Hui , Zhou Lei , Lan Ganqiu , Yang Hanchun , Wang Lixian , Wang Ligang , Liang Jing
Published: February 2023

Exosomes are biological vesicles secreted and released by cells that act as mediators of intercellular communication and play a unique role in virus infection, antigen presentation, and suppression/promotion of body immunity. Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most damaging pathogens in the pig industry and can cause reproductive disorders in sows, respiratory diseases in pigs, reduced growth performance, and other diseases leading to pig mortality. In this study, we used the PRRSV NADC30-like CHsx1401 strain to artificially infect 42-day-old pigs and isolate serum exosomes. Based on high-throughput sequencing technology, 305 miRNAs were identified in serum exosomes before and after infection, among which 33 miRNAs were significantly differentially expressed between groups (13 relatively upregulated and 20 relatively downregulated). Sequence conservation analysis of the CHsx1401 genome identified 8 conserved regions, of which a total of 16 differentially expressed (DE) miRNAs were predicted to bind to the conserved region closest to the 3′ UTR of the CHsx1401 genome, including 5 DE miRNAs capable of binding to the CHsx1401 3′ UTR (ssc-miR-34c, ssc-miR-375, ssc-miR-378, ssc-miR-486, ssc-miR-6529). Further analysis revealed that the target genes of differentially expressed miRNAs were widely involved in exosomal function-related and innate immunity-related signaling pathways, and 18 DE miRNAs (ssc-miR-4331-3p, ssc-miR-744, ssc-miR-320, ssc-miR-10b, ssc-miR-124a, ssc-miR-128, etc.) associated with PRRSV infection and immunity were screened as potential functional molecules involved in the regulation of PRRSV virus infection by exosomes.
Epithelial-to-Mesenchymal Transition and Phenotypic Marker Evaluation in Human, Canine, and Feline Mammary Gland Tumors
Authors: Sammarco Alessandro , Gomiero Chiara , Beffagna Giorgia , Cavicchioli Laura , Ferro Silvia , Michieletto Silvia , Orvieto Enrico , Patruno Marco , Zappulli Valentina
Published: February 2023

Epithelial-to-mesenchymal transition (EMT) is a process by which epithelial cells acquire mesenchymal properties. EMT has been closely associated with cancer cell aggressiveness. The aim of this study was to evaluate the mRNA and protein expression of EMT-associated markers in mammary tumors of humans (HBC), dogs (CMT), and cats (FMT). Real-time qPCR for SNAIL, TWIST, and ZEB, and immunohistochemistry for E-cadherin, vimentin, CD44, estrogen receptor (ER), progesterone receptor (PR), ERBB2, Ki-67, cytokeratin (CK) 8/18, CK5/6, and CK14 were performed. Overall, SNAIL, TWIST, and ZEB mRNA was lower in tumors than in healthy tissues. Vimentin was higher in triple-negative HBC (TNBC) and FMTs than in ER+ HBC and CMTs (p < 0.001). Membranous E-cadherin was higher in ER+ than in TNBCs (p < 0.001), whereas cytoplasmic E-cadherin was higher in TNBCs when compared with ER+ HBC (p < 0.001). A negative correlation between membranous and cytoplasmic E-cadherin was found in all three species. Ki-67 was higher in FMTs than in CMTs (p < 0.001), whereas CD44 was higher in CMTs than in FMTs (p < 0.001). These results confirmed a potential role of some markers as indicators of EMT, and suggested similarities between ER+ HBC and CMTs, and between TNBC and FMTs.
The Role of Dietary Fiber in Improving Pig Welfare
Authors: Do Sungho , Jang Jae-Cheol , Lee Geon-Il , Kim Yoo-Yong
Published: February 2023

This review aims to discuss the effects of dietary fiber sources with various levels on stereotypic behaviors in sows. There are a variety of dietary fiber sources that are supplemented to feeds for sows. However, dietary fiber sources have different physio-chemical properties, leading to controversial results in feed motivation, nutrient digestibility, and behaviors in sows fed fiber-rich diets. Findings from previous studies indicated that soluble fiber delays nutrient absorption and decreases physical activity after feeding. In addition to this, it increases volatile fatty acid production, provides energy, and prolongs the feeling of satiety. It also prevents certain stereotypies and thus is paramount to sow welfare.
Virulence Characteristics and Molecular Typing of Carbapenem-Resistant ST15 Klebsiella pneumoniae Clinical Isolates, Possessing the K24 Capsular Type
Authors: Horváth Marianna , Kovács Tamás , Kun József , Gyenesei Attila , Damjanova Ivelina , Tigyi Zoltán , Schneider György
Published: February 2023

Klebsiella pneumoniae is an opportunistic pathogen that frequently causes nosocomial and community-acquired (CA) infections. Until now, a limited number of studies has been focused on the analyses of changes affecting the virulence attributes. Genotypic and phenotypic methods were used to characterise the 39 clinical K. pneumoniae isolates; all belonged to the pan-drug resistant, widespread clone ST 15 and expressed the K24 capsule. PFGE has revealed that the isolates could be divided into three distinct genomic clusters. All isolates possessed allS and uge genes, known to contribute to the virulence of K. pneumoniae and 10.25% of the isolates showed hypermucoviscosity, 94.87% produced type 1 fimbriae, 92.3% produced type 3 fimbriae, and 92.3% were able to produce biofilm. In vivo persistence could be supported by serum resistance 46.15%, enterobactin (94.87%) and aerobactin (5.12%) production and invasion of the INT407 and T24 cell lines. Sequence analysis of the whole genomes of the four representative strains 11/3, 50/1, 53/2 and 53/3 has revealed high sequence homology to the reference K. pneumoniae strain HS11286. Our results represent the divergence of virulence attributes among the isolates derived from a common ancestor clone ST 15, in an evolutionary process that occurred both in the hospital and in the community.
Knowledge, Attitude and Practices of Self-Medication Including Antibiotics among Health Care Professionals during the COVID-19 Pandemic in Pakistan: Findings and Implications
Authors: Mustafa Zia Ul , Iqbal Shahid , Asif Hafiz Rahil , Salman Muhammad , Jabbar Sehar , Mallhi Tauqeer Hussain , Khan Yusra Habib , Sono Tiyani Milta , Schellack Natalie , Meyer Johanna C. , Godman Brian
Published: February 2023

Since the emergence of COVID-19, several different medicines including antimicrobials have been administered to patients to treat COVID-19. This is despite limited evidence of the effectiveness of many of these, fueled by misinformation. These utilization patterns have resulted in concerns for patients’ safety and a rise in antimicrobial resistance (AMR). Healthcare workers (HCWs) were required to serve in high-risk areas throughout the pandemic. Consequently, they may be inclined towards self-medication. However, they have a responsibility to ensure any medicines recommended or prescribed for the management of patients with COVID-19 are evidence-based. However, this is not always the case. A descriptive cross-sectional study was conducted among HCWs in six districts of the Punjab to assess their knowledge, attitude and practices of self-medication during the ongoing pandemic. This included HCWs working a range of public sector hospitals in the Punjab Province. A total of 1173 HCWs were included in the final analysis. The majority of HCWs possessed good knowledge regarding self-medication and good attitudes. However, 60% were practicing self-medication amid the COVID-19 pandemic. The most frequent medicines consumed by the HCWs under self-medication were antipyretics (100%), antibiotics (80.4%) and vitamins (59.9%). Azithromycin was the most commonly purchase antibiotic (35.1%). In conclusion, HCWs possess good knowledge of, and attitude regarding, medicines they purchased. However, there are concerns that high rates of purchasing antibiotics, especially “Watch” antibiotics, for self-medication may enhance AMR. This needs addressing.
Insights into the Evolution of P. aeruginosa Antimicrobial Resistance in a Patient Undergoing Intensive Therapy
Authors: Liew Kwee Chin , O’Keeffe Jessica , Rajandas Heera , Lee Yin Peng , Harris Owen , Parimannan Sivachandran , Croft Larry , Athan Eugene
Published: February 2023

Whole genome sequencing (WGS) provides insights into the evolution of antimicrobial resistance, an urgent global health threat. Using WGS, we observe evolutionary adaptation of a Pseudomonas aeruginosa strain within an immunocompromised patient undergoing antibiotic therapy. Two blood isolates (EA-86 and EA-87) from the patient evolved separate adaptations for antibiotic resistance, while sharing common adaptive mutations for host immune evasion. In EA-86, a silencing mutation in the antibiotic efflux pump repressor, NfxB, increased antibiotic resistance, while in EA-87, a similar mutation was seen in the antibiotic efflux pump repressor mexR. The number of genomic variants between the two isolates give a divergence time estimate of the order of 1000 generations. This time is sufficient for a bacterial lineage to have evolved an SNP in every position in the genome and been fixed if advantageous. This demonstrates the evolutionary adaptive power accessible to bacteria and the timescale for a brute-force functional survey of the SNP fitness landscape.
Anti-Inflammatory, Antioxidant Activities, and Phytochemical Characterization of Edible Plants Exerting Synergistic Effects in Human Gastric Epithelial Cells
Authors: Atchan Achille Parfait Nwakiban , Monthe Orissa Charlène , Tchamgoue Armelle Deutou , Singh Yeshvanthi , Shivashankara Shilpa Talkad , Selvi Moorthy Karthika , Agbor Gabriel Agbor , Magni Paolo , Piazza Stefano , Manjappara Uma Venkateswaran , Kuiate Jules-Roger , Dell’Agli Mario
Published: February 2023

Dietary bioactive compounds from natural sources (e.g., herbal medicines, foods) are known to potentially suppress acute or chronic inflammation and promote the effectiveness of treatment to reduce the harmful effects of gastritis alone or in combination. In this regard, we have characterized four Cameroonian spice extracts, namely Aframomum citratum, Dichrostachys glomerata, Tetrapleura tetraptera, and Xylopia parviflora through reverse phase-high-performance liquid chromatography (RP-HPLC), ultra-performance liquid chromatography-electrospray ionization high-resolution mass spectrometry (UPLC-ESI-HRMS/MS), and Fourier transform infrared spectroscopic (FTIR) analyses and investigated their antioxidant and synergistic anti-inflammatory activities in human gastric adenocarcinoma (AGS) and gastric epithelial (GES-1) cells. The extracts showed a high amount of total phenolic (TPC: 150–290 mg gallic acid equivalents (GAE)/g of extract) and flavonoid content (TFC: 35–115 mg catechin equivalents (CE)/g of extract) with antioxidant properties in a cell-free system (1,1-Diphenyl-2-picryl-hydrazyl (DPPH) half maximal inhibitory concentration (IC50s) ≤ 45 µg/mL; 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) half maximal inhibitory concentration (IC50s) ≤ 29 µg/mL. The extracts in combination (MIX) exert a synergistic beneficial effect (combination index (CIs) < 1 and dose reduction index (DRIs) > 1) on inflammatory markers (interleukin (IL)-8 and -6 release, and nuclear factor kappa B (NF-κB) driven transcription) in human gastric epithelial cells, which may result from the presence of phenolic compounds (phenolic acids, flavonoids) or other compounds (protein, lipid, aromatic, and polysaccharide compounds) tentatively identified in the extracts. The general findings of the present study provide supporting evidence on the chemical composition of four Cameroonian dietary plants and their significant synergistic inhibitory activities on inflammatory markers of gastric epithelial cells.
Muscle Oxidative Stress Plays a Role in Hyperthyroidism-Linked Insulin Resistance
Authors: Fasciolo Gianluca , Napolitano Gaetana , Aprile Marianna , Cataldi Simona , Costa Valerio , Muscari Tomajoli Maria Teresa , Lombardi Assunta , Di Meo Sergio , Venditti Paola
Published: February 2023

While a low level of ROS plays a role in cellular regulatory processes, a high level can lead to oxidative stress and cellular dysfunction. Insulin resistance (IR) is one of the dysfunctions in which oxidative stress occurs and, until now, the factors underlying the correlation between oxidative stress and IR were unclear and incomplete. This study aims to explore this correlation in skeletal muscle, a tissue relevant to insulin-mediated glucose disposal, using the hyperthyroid rat as a model of oxidative stress. The development of IR in the liver from hyperthyroid animals has been widely reported, whereas data concerning the muscle are quite controversial. Thus, we investigated whether hyperthyroidism induces IR in skeletal muscle and the role of oxidative stress in this process. Particularly, we compared the effects of hyperthyroidism on IR both in the absence and presence of vitamin E (Vit E), acting as an antioxidant. Putative correlations between ROS production, oxidative stress markers, antioxidant capacity and changes in intracellular signalling pathways related to insulin action (AKT) and cellular stress response (EIF2α; JNK; PGC1α; BIP; and NRF1) were investigated. Moreover, we assessed the effects of hyperthyroidism and Vit E on the expression levels of genes encoding for glucose transporters (Slc2a1; Slc2a4), factors involved in lipid homeostasis and insulin signalling (Pparg; Ppara, Cd36), as well as for one of the IR-related inflammatory factors, i.e., interleukin 1b (Il1b). Our results suggest that hyperthyroidism-linked oxidative stress plays a role in IR development in muscle and that an adequate antioxidant status, obtained by vitamin E supplementation, that mitigates oxidative stress, may prevent IR development.
Dieckol, Derived from the Edible Brown Algae Ecklonia cava, Attenuates Methylglyoxal-Associated Diabetic Nephropathy by Suppressing AGE–RAGE Interaction
Authors: Cho Chi-Heung , Yoo Guijae , Kim Mingyeong , Kurniawati Ulfah Dwi , Choi In-Wook , Lee Sang-Hoon
Published: February 2023

The formation of advanced glycation end products (AGE) is linked to the pathogenesis of diabetic nephropathy. The aim of this work was to assess the therapeutic potential and underlying mechanism of action of dieckol (DK), isolated from Ecklonia cava, on renal damage induced by methylglyoxal (MGO) in mouse glomerular mesangial cells. The antiglycation properties of DK were evaluated using ELISA. We conducted molecular docking, immunofluorescence analysis, and Western blotting to confirm the mechanism by which DK prevents AGE-related diabetic nephropathy. DK treatment exhibited antiglycation properties through the inhibition of AGE production, inhibition of cross-linking between AGE and collagen, and breaking of its cross-linking. DK pretreatment exhibited protective effects on renal cells by suppressing MGO-induced intracellular reactive oxygen species (ROS) formation, intracellular MGO and AGE accumulation, activation of the apoptosis cascade and apoptosis-related protein expression, activation of receptor for AGE (RAGE) protein expression, and suppression of the glyoxalase system. Furthermore, DK exhibited a stronger binding affinity for RAGE than AGE, which was confirmed as exerting a competitive inhibitory effect on the AGE–RAGE interaction. These results demonstrated that DK is a potential natural AGE inhibitor that can be utilized to prevent and treat AGE-induced diabetic nephropathy.
Antioxidant Materials in Oral and Maxillofacial Tissue Regeneration: A Narrative Review of the Literature
Authors: Abedi Niloufar , Sajadi-Javan Zahra Sadat , Kouhi Monireh , Ansari Legha , Khademi Abbasali , Ramakrishna Seeram
Published: February 2023

Oral and maxillofacial tissue defects caused by trauma, tumor reactions, congenital anomalies, ischemic diseases, infectious diseases, surgical resection, and odontogenic cysts present a formidable challenge for reconstruction. Tissue regeneration using functional biomaterials and cell therapy strategies has raised great concerns in the treatment of damaged tissue during the past few decades. However, during biomaterials implantation and cell transplantation, the production of excessive reactive oxygen species (ROS) may hinder tissue repair as it commonly causes severe tissue injuries leading to the cell damage. These products exist in form of oxidant molecules such as hydrogen peroxide, superoxide ions, hydroxyl radicals, and nitrogen oxide. These days, many scientists have focused on the application of ROS-scavenging components in the body during the tissue regeneration process. One of these scavenging components is antioxidants, which are beneficial materials for the treatment of damaged tissues and keeping tissues safe against free radicals. Antioxidants are divided into natural and synthetic sources. In the current review article, different antioxidant sources and their mechanism of action are discussed. The applications of antioxidants in the regeneration of oral and maxillofacial tissues, including hard tissues of cranial, alveolar bone, dental tissue, oral soft tissue (dental pulp, periodontal soft tissue), facial nerve, and cartilage tissues, are also highlighted in the following parts.
The Role of Lonp1 on Mitochondrial Functions during Cardiovascular and Muscular Diseases
Authors: Zanini Giada , Selleri Valentina , Malerba Mara , Solodka Kateryna , Sinigaglia Giorgia , Nasi Milena , Mattioli Anna Vittoria , Pinti Marcello
Published: February 2023

The mitochondrial protease Lonp1 is a multifunctional enzyme that regulates crucial mitochondrial functions, including the degradation of oxidized proteins, folding of imported proteins and maintenance the correct number of copies of mitochondrial DNA. A series of recent studies has put Lonp1 at the center of the stage in the homeostasis of cardiomyocytes and muscle skeletal cells. During heart development, Lonp1 allows the metabolic shift from anaerobic glycolysis to mitochondrial oxidative phosphorylation. Knock out of Lonp1 arrests heart development and determines cardiomyocyte apoptosis. In adults, Lonp1 acts as a cardioprotective protein, as its upregulation mitigates cardiac injury by preventing the oxidative damage of proteins and lipids, and by preserving mitochondrial redox balance. In skeletal muscle, Lonp1 is crucial for cell development, as it mediates the activation of PINK1/Parkin pathway needed for proper myoblast differentiation. Skeletal muscle-specific ablation of Lonp1 in mice causes reduced muscle fiber size and strength due to the accumulation of mitochondrial-retained protein in muscle. Lonp1 expression and activity decline with age in different tissues, including skeletal muscle, and are associated with a functional decline and structural impairment of muscle fibers. Aerobic exercise increases unfolded protein response markers including Lonp1 in the skeletal muscle of aged animals and is associated with muscle functional recovery. Finally, mutations of Lonp1 cause a syndrome named CODAS (Cerebral, Ocular, Dental, Auricular, and Skeletal anomalies) characterized by the impaired development of multiple organs and tissues, including myocytes. CODAS patients show hypotonia and ptosis, indicative of skeletal muscle reduced performance. Overall, this body of observations points Lonp1 as a crucial regulator of mitochondrial functions in the heart and in skeletal muscle.
Dietary Sodium and Nonalcoholic Fatty Liver Disease: A Systematic Review
Authors: da Silva Ferreira Guilherme , Catanozi Sergio , Passarelli Marisa
Published: February 2023

(1) Introduction: Restriction in sodium intake is an important strategy for reducing cardiovascular morbidity and mortality, considering the direct influence of high-sodium diet consumption on the development of hypertension and cardiovascular diseases. There are only a few studies dealing with the influence of dietary sodium on the development of nonalcoholic fatty liver disease (NAFLD). In this systematic review, evidence in humans and animal models was compiled in a critical view of the influence of dietary sodium intake patterns on NAFLD markers; (2) Methods: Systematic review of PubMed data. Clinical outcomes included the prevalence/incidence of NAFLD for human studies, and NAFLD markers (hepatic lipogenesis, and markers of steatosis, fibrosis, and inflammation) for animal studies. The protocol was registered at the International Prospective Register of Systematic Review (PROSPERO; CRD42023390447); (3) Results and Conclusion: Seven studies in humans and eight in animals were included. All studies in humans were observational and associated high-sodium intake with NAFLD. However, in animals, both the increased and reduced consumption of sodium negatively influenced markers of liver steatosis, inflammation, and fibrosis.
Arrays of TiO2 Nanosphere Monolayers on GaN-Based LEDs for the Improvement of Light Extraction
Authors: Kim Dohyun , Jung UiJin , Heo Wonjun , Kumar Navneet , Park Jinsub
Published: February 2023

We report on the fabrication of TiO2 nanosphere (NS) monolayer arrays for the enhancement of light extraction quantum efficiency of GaN-based light-emitting diodes (LEDs). The fabricated TiO2 NSs monolayer arrays were composed of different phases of anatase (An-) and amorphous (Am-) TiO2. The arrays were transferred onto the topmost layer of LED chips via the facile icing transfer method. The LED chips covered with Am-TiO2 NS monolayer arrays showed 3.0- times enhanced light output power intensity compared with reference LED chips at a fixed injection current of 100 mA. The enhanced light extraction of LED chips by an Am-TiO2 NS monolayer can be attributed to a high transmittance (91.1%) in visible and increased light extraction probability of photons generated in LEDs, resulting from the enhanced light coupling efficiency by reduced total internal reflection (TIR). Finite-difference time-domain (FDTD) simulation results also agreed well with the experimentally observed results. Based on the experimental and theoretical results, our suggested Am- and An-TiO2 NS arrays can be considered a very facile and effective method to improve the device performance of various visible LED chips.
A Review of Degradation and Life Prediction of Polyethylene
Authors: Wang Yang , Feng Guowei , Lin Nan , Lan Huiqing , Li Qiang , Yao Dichang , Tang Jing
Published: February 2023

After around 50 years of development, the key substance known as polyethylene has been extremely influential in a variety of industries. This paper investigates how polyethylene materials have been used in the domains of water, packaging, and medicine to advance contemporary society in order to comprehend the physical and chemical alterations that polyethylene undergoes after being subjected to long-term environmental variables (e.g., temperature, light, pressure, microbiological factors, etc.). For the safe operation of polyethylene materials, it has always been of the utmost importance to evaluate polyethylene’s service life effectively. This paper reviews some of the most common literature journals on the influence of environmental factors on the degradation process of polyethylene materials and describes methods for predicting the lifetime of degradable polyethylene materials using accelerated aging tests. The Arrhenius equation, the Ozawa–Flynn–Wall (OFW) method, the Friedman method, the Coats–Redfern method, the Kissinger method and Kissinger–Akahira–Sunose (KAS) method, Augis and Bennett’s method, and Advanced Isoconversional methods are all discussed, as well as the future development of polyethylene.
Special Issue: “Turbomachinery: Theory, Design and Application”
Authors: Choi Minsuk , Kim Jin-Hyuk , Wu Dazhuan
Published: February 2023

Turbomachinery is an essential part of the industrial field, and it is usually used for transporting fluids, extracting energy from flows, and cooling heated surfaces in many industrial sites and inside machines [...]
Exploring the Keyword Network Analysis and Research Trends for Sustainable Development of Paralympic Research: Based on Changes in Classification
Authors: Kim Min-Chang
Published: February 2023

This study aims to identify an overall keyword network by drawing on core keywords in Paralympic studies and analyzing the relationships. It also aims to provide information on the changes in Paralympic study trends through a time series analysis to understand change, according to the application of a new classification (evidence-based classification). This study made a bibliographic list of 1613 studies by collecting studies published between 2003 and 2022 using keyword network analysis (KNA). By refining to exclude unsuitable studies, 1404 studies and 6414 keywords were used for the analysis. As a result of analyzing entire Paralympic studies performed between 2003 and 2022, Paralympic studies were revealed to be on the rise. The entire Paralympic studies ascertained that the areas of study were mainstream and centered on the top three keywords: athlete; performance; and classification. Secondly, as a result of analyzing Paralympic study trends in a time series manner, based on 2017 when a new classification was applied, studies related to physical impairment centered on the top two keywords, athlete and performance, were mainstream in Section 1 (2003–2016). Olympic Games, performance, and wheelchairs formed detailed study areas. In the Paralympic studies in Section 2 (2017–2022), the relevant studies centered on the top two keywords, athletes and performance, were mainstream. Parasport, disability, and classification were ascertained to form detailed study areas. Through all this, the changed trend in the keyword Paralympics was confirmed. The results of this study are meaningful in that they can contribute to the expansion of the Paralympic areas of study and invigoration by exploring core keywords and providing relevant information based on the significant change point in time, including entire Paralympic study trends.