bluuebunny/crossref_metadata_2025 huggingface dataset:
High levels of dietary methionine improves sitagliptin-induced hepatotoxicity by attenuating oxidative stress in hypercholesterolemic rats
Authors: Kumar Avinash , Pathak Rashmi , Palfrey Henry A. , Stone Kirsten P. , Gettys Thomas W. , Murthy Subramanyam N.
Published: January 2020

Background

Both cholesterol (Cho) and methionine (Met, a precursor for homocysteine) are risk factors for fatty liver disease. Since Western diets are rich in Cho and Met, we investigated the hepatic effects of feeding a diet enriched in Met and Cho. Further, based on the reported anti-oxidative and lipid lowering properties of sitagliptin (an antidiabetic drug), we tested whether it could counteract the negative effects of high Cho and Met. We therefore hypothesized that sitagliptin would ameliorate the development of liver pathology that is produced by feeding diets rich in either Cho, Met, or both.

Methods

Male Sprague Dawley rats were fed ad libitum a) control diet, or b) high Met or c) high Cho, or d) high Met + high Cho diets for 35 days. From day 10 to 35, 50% of rats in each dietary group were gavaged with either vehicle or an aqueous suspension of sitagliptin (100 mg/kg/day). Liver samples were harvested for histological, molecular, and biochemical analyses.

Results

The high Cho diet produced significant hepatic steatosis which was unaffected by sitagliptin. Contrary to expectation, sitagliptin exacerbated expression of hepatic markers of oxidative stress and fibrosis in rats fed high Cho. Corresponding increases in 4-hydroxynonenal adducts and collagen deposition were demonstrated by immunohistochemistry and sirius red staining. These hepatic changes were absent in rats on the high Met diet and they were comparable to controls. The inclusion of Met in the high Cho diet resulted in significant reduction of the hepatic steatosis, oxidative stress, and fibrosis produced by high Cho alone.

Conclusion

Sitagliptin exacerbated the effects of high Cho on both oxidative stress and fibrosis, resulting in NASH like symptoms that were significantly reversed by the inclusion of Met.
Effectiveness of a new approach for exclusive breastfeeding counselling on breastfeeding prevalence, infant growth velocity and postpartum weight loss in overweight or obese women: protocol for a randomized controlled trial
Authors: Aldana-Parra Fanny , Olaya Gilma , Fewtrell Mary
Published: January 2020

Background

Maternal overweight, infant feeding and early growth velocity are risk factors for obesity later in life. The first one thousand days are a window of opportunity to program health and disease. Exclusive breastfeeding may protect against obesity; however, it is not consistently practiced. Obesity rates have been increasing worldwide. Overweight or obese women have lower rates of breastfeeding and face mechanical, psychological and biological difficulties. Breastfeeding counselling is a successful strategy to support breastfeeding in normal weight women; but there is a lack of evidence on its effectiveness in overweight women. Our purpose is to evaluate a new approach to exclusive breastfeeding counselling based on Carl Rogers’ Centred-Client Theory in overweight women, and to examine effects on breastfeeding prevalence, infant growth velocity and maternal postpartum weight loss.

Methods

A two-arm simple randomized controlled trial will be conducted in overweight and obese women recruited in a Baby Friendly Hospital in Bogotá, Colombia. The intervention is exclusive breastfeeding counselling based on Rogers’ theory but adapted for overweight women; it will be performed during the last month of pregnancy, 24 h after delivery and during early infancy (1 and 3 months postpartum). The primary outcomes will be exclusive breastfeeding prevalence, infant growth velocity and maternal weight loss from birth up to 4 months after delivery; and the secondary outcomes will be prolactin and macronutrient levels in breast milk and serum prolactin levels. Intention to treat analysis will be performed to estimate the effect of the new counselling approach compared to standard management on the prevalence of exclusive breastfeeding, infant growth velocity and maternal weight loss.

Discussion

We hypothesize that the intervention will result in an increase in the initiation and maintenance of exclusive breastfeeding, allowing adequate infant growth velocity and maternal weight loss after delivery. It is hoped that the results of this trial will provide evidence to support public health policy on supporting breastfeeding in this vulnerable group of women.

Trial registration

(UTN) U1111–1228-9913 February 20th 2019; ISRCTN15922904

February 27th 2019, retrospectively registered.
Definitive chemoradiotherapy in patients with squamous cell cancers of the head and neck - results from an unselected cohort of the clinical cooperation group “Personalized Radiotherapy in Head and Neck Cancer”
Authors: Schüttrumpf Lars , Marschner Sebastian , Scheu Katrin , Hess Julia , Rietzler Sibylle , Walch Axel , Baumeister Philipp , Kirchner Thomas , Ganswindt Ute , Zitzelsberger Horst , Belka Claus , Maihoefer Cornelius
Published: January 2020

Background

Definitive chemoradiotherapy (dCRT) is a standard treatment for patients with locally advanced head and neck cancer. There is a clinical need for a stratification of this prognostically heterogeneous group of tumors in order to optimize treatment of individual patients. We retrospectively reviewed all patients with head and neck squamous cell carcinoma (HNSCC) of the oral cavity, oropharynx, hypopharynx, or larynx, treated with dCRT from 09/2008 until 03/2016 at the Department of Radiation Oncology, LMU Munich. Here we report the clinical results of the cohort which represent the basis for biomarker discovery and molecular genetic research within the framework of a clinical cooperation group.

Methods

Patient data were collected and analyzed for outcome and treatment failures with regard to previously described and established risk factors.

Results

We identified 184 patients with a median follow-up of 65 months and a median age of 64 years. Patients received dCRT with a median dose of 70 Gy and simultaneous chemotherapy in 90.2% of cases, mostly mitomycin C / 5-FU in concordance with the ARO 95–06 trial. The actuarial 3-year overall survival (OS), local, locoregional and distant failure rates were 42.7, 29.8, 34.0 and 23.4%, respectively. Human papillomavirus-associated oropharynx cancer (HPVOPC) and smaller gross tumor volume were associated with significantly improved locoregional tumor control rate, disease-free survival (DFS) and OS in multivariate analysis. Additionally, lower hemoglobin levels were significantly associated with impaired DFS und OS in univariate analysis. The extent of lymph node involvement was associated with distant failure, DFS and OS. Moreover, 92 patients (50%) of our cohort have been treated in concordance with the ARO 95–06 study, corroborating the results of this study.

Conclusion

Our cohort is a large unselected monocentric cohort of HNSCC patients treated with dCRT. Tumor control rates and survival rates compare favorably with the results of previously published reports. The clinical data, together with the available tumor samples from biopsies, will allow translational research based on molecular genetic analyses.
HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR
Authors: Maroun Christopher A. , Al Feghali Karine , Traboulsi Henri , Dabbous Helene , Abbas Fatmeh , Dunya Gabriel , Ziade Georges , Mahfouz Rami , Youssef Bassem , Tamim Hani , Geara Fady , Khalifeh Ibrahim , Moukarbel Roger V.
Published: January 2020

Background

Given the paucity of data and widely variable rates that have been reported, the main objective of this study was to examine the prevalence of HPV-positivity in oropharyngeal squamous cell carcinoma (OPSCC) in Middle Eastern patients presenting to one of the region’s largest tertiary care centers using polymerase chain reaction (PCR) amplification of the HPV E6/E7 oncogenes, a highly sensitive and specific method of detection.

Methods

Medical charts and archived pathological specimens were obtained for patients diagnosed with biopsy proven oropharyngeal cancer who presented to the American University of Beirut Medical Center between 1972 and 2017. DNA was extracted from paraffin-embedded specimens and tested for 30 high-risk and low-risk papilloma viruses using the PCR-based EUROarray HPV kit (EuroImmun).

Results

A total of 57 patients with oropharyngeal cancer were initially identified; only 34 met inclusion/exclusion criteria and were included in the present study. Most patients were males (73.5%) from Lebanon (79.4%). The most common primary tumor site was in the base of tongue (50%), followed by the tonsil (41.2%). The majority of patients (85.3%) tested positive for HPV DNA.

Conclusion

The prevalence of HPV-positivity amongst Middle Eastern OPSCC patients, specifically those from Lebanon, may be far greater than previously thought. The Lebanese population and other neighboring Middle Eastern countries may require a more vigilant approach towards HPV detection and awareness. On an international level, further research is required to better elucidate non-classical mechanisms of HPV exposure and transmission.
Isolated auditory neuropathy at birth in congenital cytomegalovirus infection
Authors: Natale Fabio , De Curtis Mario , Bizzarri Bianca , Orlando Maria Patrizia , Ralli Massimo , Liuzzi Giuseppina , Caravale Barbara , Franco Francesco , Gaeta Aurelia , Giancotti Antonella , Russo Francesca Yoshie , Turchetta Rosaria
Published: January 2020

Background

Congenital cytomegalovirus (cCMV) infection is the most frequent non-genetic cause of sensorineural hearing-loss (SNHL) (i.e., hearing loss due to a cochlear and/or auditory nerve damage). It is widely accepted that SNHL at birth, when associated to cCMV symptomatic infection involving the central nervous system, benefits from antiviral therapy started in the neonatal period. Conversely, there is no consensus for antiviral treatment in congenitally infected infants diagnosed with isolated SNHL (i.e., SNHL in an otherwise asymptomatic infant) at birth.

Our aim was to assess the frequency and the auditory outcome of isolated SNHL at birth due to auditory neuropathy (AN) (i.e., SNHL in a patient with normal cochlear function and auditory nerve dysfunction) in infants with cCMV infection.

Methods

We retrospectively reviewed the clinical history of 60 infants, born at term, with cCMV asymptomatic infection, without additional risk factors for SNHL, and exhibiting bilateral “pass” otoacustic emissions (OAE). None of them underwent antiviral therapy.

Hearing thresholds were assessed by means of Auditory Brainstem Responses (ABR). AN affected children were followed up until possible normalization of the hearing thresholds or definitive diagnosis of AN. Each infant diagnosed with monolateral or bilateral AN was classified according to the worst ear threshold.

Results

In our population, the first ABR was performed at a mean age of 5.00 ± 2.79 (SD) months and AN was diagnosed in 16/60 (26.67%) infants; in 4 infants the AN was defined as mild (4/4 monolateral), moderate in 11 (5/11 bilateral), and severe in 1 (bilateral). The mean age at first ABR was 3.69 ± 2.80 (SD) months in the 16 babies with AN and 5.48 ± 2.66 (SD) months in the 44 infants with normal hearing (p = 0.007). All AN cases spontaneously recovered a normal auditory threshold over time. The mean length of the audiological follow-up was 32.44 ± 17.58 (SD) months (range 5–60 months).

Conclusion

A delayed maturation of the auditory pathways should be considered when a mild/moderate isolated AN at birth is detected in cCMV infected infants. Prospective studies conducted on larger populations, and with a longer audiological follow-up, are needed to confirm our findings.
Blood biomarkers as surrogate endpoints of treatment responses to aerobic exercise and cognitive training (ACT) in amnestic mild cognitive impairment: the blood biomarkers study protocol of a randomized controlled trial (the ACT Trial)
Authors: Li Danni , Mielke Michelle M. , Bell W. Robert , Reilly Cavan , Zhang Lin , Lin Feng Vankee , Yu Fang
Published: January 2020

Background

Alzheimer’s disease (AD) is an epidemic with tremendous public health impacts because there are currently no disease-modifying therapeutics. Randomized controlled trials (RCTs) for prevention of AD dementia often use clinical endpoints that take years to manifest (e.g., cognition) or surrogate endpoints that are costly or invasive (e.g., magnetic resonance imaging [MRI]). Blood biomarkers represent a clinically applicable alternative surrogate endpoint for RCTs that would be both cost-effective and minimally invasive, but little is known about their value as surrogate endpoints for treatment responses in the prevention of AD dementia.

Methods

The objective of this study is to investigate blood neuropathological, neurodegenerative, and neurotrophic biomarkers as surrogate endpoints for treatment responses to three interventions in older adults with amnestic mild cognitive impairment (aMCI, a prodromal stage of AD): aerobic exercise, cognitive training, and combined aerobic exercise and cognitive training (ACT). We chose these three sets of biomarkers for their unique mechanistic associations with AD pathology, neurodegeneration and neurogenesis. This study is built on the ACT Trial (1R01AG055469), a single-blinded, multi-site, 2 × 2 factorial phase II RCT that examines the synergistic effects of a 6-month ACT intervention on cognition and MRI biomarkers (AD-signature cortical thickness and hippocampal volume) (n = 128). In this ACT Trial blood biomarkers study, we will enroll 120 ACT Trial participants with aMCI and measure blood biomarkers at baseline and at 3, 6, 12, and 18 months. The goals are to (1) determine the effect of interventions on blood biomarkers over 6 months, (2) evaluate blood biomarkers as surrogate endpoints for predicting cognitive responses to interventions over 18 months, and (3, exploratory) examine blood biomarkers as surrogate endpoints for predicting brain MRI biomarker responses to interventions over 18 months.

Discussion

This study aims to identify new blood biomarkers that can track cognitive decline or AD-related brain atrophy among patients with aMCI subjected to a regimen of aerobic exercise and cognitive training. Findings from this study will drive the further use of blood biomarkers in developing effective prevention and treatment strategies for AD dementia.

Trial registration

ClinicalTrials.gov, NCT03313895. Registered on 18 October 2017.
Protocol for a randomized controlled trial examining multilevel prediction of response to behavioral activation and exposure-based therapy for generalized anxiety disorder
Authors: Santiago J. , Akeman E. , Kirlic N. , Clausen A. N. , Cosgrove K. T. , McDermott T. J. , Mathis B. , Paulus M. , Craske M. G. , Abelson J. , Martell C. , Wolitzky-Taylor K. , Bodurka J. , Thompson W. K. , Aupperle Robin L.
Published: January 2020

Background

Only 40–60% of patients with generalized anxiety disorder experience long-lasting improvement with gold standard psychosocial interventions. Identifying neurobehavioral factors that predict treatment success might provide specific targets for more individualized interventions, fostering more optimal outcomes and bringing us closer to the goal of “personalized medicine.” Research suggests that reward and threat processing (approach/avoidance behavior) and cognitive control may be important for understanding anxiety and comorbid depressive disorders and may have relevance to treatment outcomes. This study was designed to determine whether approach-avoidance behaviors and associated neural responses moderate treatment response to exposure-based versus behavioral activation therapy for generalized anxiety disorder.

Methods/design

We are conducting a randomized controlled trial involving two 10-week group-based interventions: exposure-based therapy or behavioral activation therapy. These interventions focus on specific and unique aspects of threat and reward processing, respectively. Prior to and after treatment, participants are interviewed and undergo behavioral, biomarker, and neuroimaging assessments, with a focus on approach and avoidance processing and decision-making. Primary analyses will use mixed models to examine whether hypothesized approach, avoidance, and conflict arbitration behaviors and associated neural responses at baseline moderate symptom change with treatment, as assessed using the Generalized Anxiety Disorder–7 item scale. Exploratory analyses will examine additional potential treatment moderators and use data reduction and machine learning methods.

Discussion

This protocol provides a framework for how studies may be designed to move the field toward neuroscience-informed and personalized psychosocial treatments. The results of this trial will have implications for approach-avoidance processing in generalized anxiety disorder, relationships between levels of analysis (i.e., behavioral, neural), and predictors of behavioral therapy outcome.

Trial registration

The study was retrospectively registered within 21 days of first participant enrollment in accordance with FDAAA 801 with ClinicalTrials.gov,NCT02807480. Registered on June 21, 2016, before results.
Exploring the recruitment, ethical considerations, conduct and information dissemination of an audiology trial: a pretrial qualitative study (q-COACH)
Authors: Francis-Auton Emilie , Warren Chris , Braithwaite Jeffrey , Rapport Frances
Published: January 2020

Introduction

Randomised controlled trials (RCTs), while still considered the gold standard approach in medical research, can encounter impediments to their successful conduct and the dissemination of results. Pretrial qualitative research can usefully address some of these impediments, including recruitment and retention, ethical conduct, and preferred methods of dissemination. However, pretrial qualitative work is rarely undertaken in audiology. The Comparison of outcomes with hearing aids and cochlear implants in adults with moderately severe-to-profound bilateral sensorineural hearing loss (COACH) is a proposed RCT aiming to clarify when hearing aids (HAs) or cochlear implants (CIs) are the most suitable for different degrees of hearing loss and for which kinds of patients. q-COACH is a pretrial, qualitative study examining stakeholders’ experiences of HAs and CIs, current clinical practices and stakeholders’ perspectives of the design, conduct and dissemination plans for the proposed COACH study.

Methods

Twenty-four participants including general practitioners, audiologists, adult HA users, and adult support networks undertook either semi-structured individual or paired interviews and completed demographic questionnaires. Data were analysed thematically.

Results

Four key themes arose from this study: 1) rethinking sampling and recruitment strategies, 2) ethical considerations, 3) refining trial conduct, and 4) interconnected, appropriate and accessible methods of results dissemination.

Conclusions

This qualitative investigation identified key considerations for the proposed RCT design, conduct and dissemination to help with successful implementation of COACH, and to indicate a plan of action at all RCT stages that would be acceptable to potential participants. By drawing on the perspectives of multiple key stakeholders and including a more general discussion of their experience and opinions of hearing loss, hearing device use and service availability, the study revealed experiential and ethical paradigms in which stakeholders operate. In so doing, q-COACH has exposed the benefits of preliminary qualitative investigations that enable detailed and rich understandings of the phenomenon at stake, forestalling problems and improving the quality of trial design, conduct and dissemination, while informing future RCT development discussions.
Use of mepolizumab in adult patients with cystic fibrosis and an eosinophilic phenotype: case series
Authors: Zhang Lijia , Borish Larry , Smith Anna , Somerville Lindsay , Albon Dana
Published: January 2020

Background

Cystic fibrosis (CF) is characterized by inflammation, progressive lung disease, and respiratory failure. Although the relationship is not well understood, patients with CF are thought to have a higher prevalence of asthma than the general population. CF Foundation (CFF) annual registry data in 2017 reported a prevalence of asthma in CF of 32%. It is difficult to differentiate asthma from CF given similarities in symptoms and reversible obstructive lung function in both diseases. However, a specific asthma phenotype (type 2 inflammatory signature), is often identified in CF patients and this would suggest potential responsiveness to biologics targeting this asthma phenotype. A type 2 inflammatory condition is defined by the presence of an interleukin (IL)-4high, IL-5high, IL-13high state and is suggested by the presence of an elevated total IgE, specific IgE sensitization, or an elevated absolute eosinophil count (AEC). In this manuscript we report the effects of using mepolizumab in patients with CF and type 2 inflammation.

Results

We present three patients with CF (63, 34 and 24 year of age) and personal history of asthma, who displayed significant eosinophilic inflammation and high total serum IgE concentrations (type 2 inflammation) who were treated with mepolizumab. All three patients were colonized with multiple organisms including Pseudomonas aeruginosa and Aspergillus fumigatus and tested positive for specific IgE to multiple allergens. We examined the effect of mepolizumab on patients’ lung function (FEV1), blood markers of type 2 inflammation, systemic corticosteroid use and frequency of CF exacerbations. One patient had a substantial increase in lung function after starting mepolizumab and all three patients had a substantial benefit in regards to reduced oral CCS use. While none of the patients showed significant changes in the exacerbation rates there was markedly reduced requirements for oral CCS with exacerbations. In addition, mepolizumab had a positive effect on type 2 inflammatory markers, reducing markers of allergic inflammation in all 3 patients.

Conclusions

Mepolizumab appears to have a positive effect on clinical course in patients with CF presenting with a type 2 phenotype characterized by allergic sensitization and hyper-eosinophilia.
Paramedics and Physician Assistants in Israel
Authors: Hooker Roderick S.
Published: January 2020

Israeli emergency medicine is undergoing change. The paramedic is experiencing high separation rates because the position is understaffed, overworked, and underpaid. Physician assistants (PAs) were introduced into the emergency department by training paramedics and to date they seem satisfied with this new role. Experience in other countries indicates that PAs can improve access to care, reduce errors, increase efficiency and have satisfying roles in health systems. The Israeli health system will need to determine if additional roles for PAs will be accepted by the public and physicians alike.
Prevention and treatment of acute lung injury with time-controlled adaptive ventilation: physiologically informed modification of airway pressure release ventilation
Authors: Nieman Gary F. , Gatto Louis A. , Andrews Penny , Satalin Joshua , Camporota Luigi , Daxon Benjamin , Blair Sarah J. , Al-khalisy Hassan , Madden Maria , Kollisch-Singule Michaela , Aiash Hani , Habashi Nader M.
Published: January 2020

Mortality in acute respiratory distress syndrome (ARDS) remains unacceptably high at approximately 39%. One of the only treatments is supportive: mechanical ventilation. However, improperly set mechanical ventilation can further increase the risk of death in patients with ARDS. Recent studies suggest that ventilation-induced lung injury (VILI) is caused by exaggerated regional lung strain, particularly in areas of alveolar instability subject to tidal recruitment/derecruitment and stress-multiplication. Thus, it is reasonable to expect that if a ventilation strategy can maintain stable lung inflation and homogeneity, regional dynamic strain would be reduced and VILI attenuated. A time-controlled adaptive ventilation (TCAV) method was developed to minimize dynamic alveolar strain by adjusting the delivered breath according to the mechanical characteristics of the lung. The goal of this review is to describe how the TCAV method impacts pathophysiology and protects lungs with, or at high risk of, acute lung injury. We present work from our group and others that identifies novel mechanisms of VILI in the alveolar microenvironment and demonstrates that the TCAV method can reduce VILI in translational animal ARDS models and mortality in surgical/trauma patients. Our TCAV method utilizes the airway pressure release ventilation (APRV) mode and is based on opening and collapsing time constants, which reflect the viscoelastic properties of the terminal airspaces. Time-controlled adaptive ventilation uses inspiratory and expiratory time to (1) gradually “nudge” alveoli and alveolar ducts open with an extended inspiratory duration and (2) prevent alveolar collapse using a brief (sub-second) expiratory duration that does not allow time for alveolar collapse. The new paradigm in TCAV is configuring each breath guided by the previous one, which achieves real-time titration of ventilator settings and minimizes instability induced tissue damage. This novel methodology changes the current approach to mechanical ventilation, from arbitrary to personalized and adaptive. The outcome of this approach is an open and stable lung with reduced regional strain and greater lung protection.
Mapping evidence on women’s knowledge and practice of breast self-examination in sub-Saharan Africa: a scoping review protocol
Authors: Udoh Roseline H. , Ansu-Mensah Monica , Tahiru Mohammed , Bawontuo Vitalis , Kuupiel Desmond
Published: January 2020

Background

Globally, breast cancer is the most common malignant condition in women. Breast self-examination practice following correct procedure potentially can lead to early detection of breast abnormalities. We propose to systematically chart literature and examine the scope of evidence on women’s knowledge and practice of breast self-examination in sub-Saharan Africa (SSA).

Methods

Our scoping review methods will be guided by the framework proposed by Arksey and O'Malley, Levac et al. and Joanna Briggs Institute guidelines. Literature searches will be conducted in the following electronic databases (from 2008 onwards): PubMed/MEDLINE, Scopus, Web of Science, CINAHL, PsycINFO and Health Sources. Grey literature will be identified through searching dissertation databases, Google Scholar and governmental databases. Two reviewers will screen all citations and full-text articles We will abstract data, organise them into themes and sub-themes, summarise them and report the results using a narrative synthesis. The study methodological quality (or bias) will be appraised using a mixed-method appraisal tool.

Discussion

The findings from the scoping review will contribute to obtain an understanding of the women’s knowledge and practice of breast self-examination in sub-Saharan Africa, and will likely reveal the depth of evidence helping to identify gaps for future research. Results will be published in a peer-reviewed journal. Implications for clinical practice and health policy will be discussed.
Measuring women’s experiences of maternity care: protocol for a systematic review of self-report survey instruments
Authors: Beecher Claire , Greene Richard , O’Dwyer Laura , Ryan Ethel , White Mark , Beattie Michelle , Devane Declan
Published: January 2020

Background

The use of survey instruments to measure women’s experiences of their maternity care is regarded internationally as an indicator of the quality of care received. To ensure the credibility of the data arising from these instruments, the methodological quality of development must be high. This paper reports the protocol for a systematic review of self-report instruments used to measure women’s experiences of their maternity care.

Methods

Citation databases CINAHL, Ovid MEDLINE and EMBASE will be searched from 2002 to 2018 using keywords including women, experience, maternity care, questionnaires, surveys, and self-report. Citations will be screened by two reviewers, in two rounds, for inclusion as per predetermined inclusion and exclusion criteria. Data extraction forms will be populated with data, extracted from each study, to evaluate the methodological quality of each survey instrument and the criteria for good measurement properties using quality criteria. Data will also be extracted to categorise the items included in each survey instrument. A combination of a structured narrative synthesis and quantitate summaries in tabular format will allow for recommendations to be made on the use, adaptation and development of future survey instruments.

Discussion

The value of survey instruments that evaluate women’s experiences of their maternity care, as a marker of quality care, has been recognised internationally with many countries employing the use of such instruments to inform policy and practice. The development of these instruments must be methodologically sound and the instrument itself fit for the purpose and context in which it is used. This protocol describes the methods that will be used to complete a systematic review that will serve as a guide for choosing the most appropriate existing instruments to use or adapt so that they are fit for purpose, in addition to informing the development of new instruments.

Systematic review registration

PROSPERO CRD42018105325
Chlortetracycline inhibits seed germination and seedling growth in Brassica campestris by disrupting H2O2 signaling
Authors: Cheong Mi Sun , Yoon Young-Eun , Kim Jin Wook , Hong Young Kyu , Kim Sung Chul , Lee Yong Bok
Published: January 2020

Antibiotics have been identified as a new type of environmental contaminant because of their increased use in farm animal production systems. Those drugs that animals are not absorbed mostly are excreted in the feces and urine and contaminates soils. However, the effects of antibiotics on crop plants are still largely unknown. In this study, we determined the effects of chlortetracycline (CTC), a veterinary drug released into the agricultural field by grazing animals or through manure application, on the growth and physiology of Brassica campestris seedlings. Differently from animals, Brassica campestris seedlings have accumulated 5–10-fold higher CTC during cultivation rather than excretion. Morphologically, CTC delays seed germination and inhibits seedling growth such as shortening primary root length and decreasing chlorophyll level. At the molecular level, CTC accumulation in plants downregulated the expression of superoxide dismutase (SOD) genes and decreased the production of hydrogen peroxide (H2O2). Since H2O2 is one of the signaling components involved in the regulation of root growth, exogenous application of H2O2 partially restored the growth and physiology of CTC-treated seedlings. These results suggest that application of CTC-containing manure or compost to soil delays seed germination and inhibits plant growth.
FTY720, a sphingosine analog, altered placentome histoarchitecture in ewes
Authors: Dunlap Kathrin A. , White Bryan G. , Erikson David W. , Satterfield M. Carey , Pfarrer Christiane , Wu Guoyao , Bazer Fuller W. , Burghardt Robert C. , Bayless Kayla J. , Johnson Greg A.
Published: January 2020

Background

The lysosphingolipid, sphingosine-1-phosphate, is a well-described and potent pro-angiogenic factor. Receptors, as well as the sphingosine phosphorylating enzyme sphingosine kinase 1, are expressed in the placentomes of sheep and the decidua of rodents; however, a function for this signaling pathway during pregnancy has not been established. The objective of this study was to investigate whether sphingosine-1-phosphate promoted angiogenesis within the placentomes of pregnant ewes. Ewes were given daily jugular injections of FTY720 (2-amino-2[2-(− 4-octylphenyl)ethyl]propate-1,3-diol hydrochloride), an S1P analog.

Results

FTY720 infusion from days 30 to 60 of pregnancy did not alter maternal organ weights nor total number or mass of placentomes, but did alter placentome histoarchitecture. Interdigitation of caruncular crypts and cotyledonary villi was decreased, as was the relative area of cotyledonary tissue within placentomes. Also, the percentage of area occupied by cotyledonary villi per unit of placentome was increased, while the thickness of the caruncular capsule was decreased in ewes treated with FTY720. Further, FTY720 infusion decreased the number and density of blood vessels within caruncular tissue near the placentome capsule where the crypts emerge from the capsule. Finally, FTY720 infusion decreased asparagine and glutamine in amniotic fluid and methionine in allantoic fluid, and decreased the crown rump length of day 60 fetuses.

Conclusions

While members of the sphingosine-1-phosphate signaling pathway have been characterized within the uteri and placentae of sheep and mice, the present study uses FTY720 to address the influence of S1P signaling on placental development. We present evidence that modulation of the S1P signaling pathway results in the alteration of caruncular vasculature, placentome architecture, abundance of amino acids in allantoic and amniotic fluids, and fetal growth during pregnancy in sheep. The marked morphological changes in placentome histoarchitecture, including alteration in the vasculature, may be relevant to fetal growth and survival. It is somewhat surprising that fetal length was reduced as early as day 60, because fetal growth in sheep is greatest after day 60. The subtle changes observed in the fetuses of ewes exposed to FTY720 may indicate an adaptive response of the fetuses to cope with altered placental morphology.
The effects of oral administration of Cola nitida on the pharmacokinetic profile of metoclopramide in rabbits
Authors: Amadi Cecilia Nwadiuto , Nwachukwu Wisdom Izuchukwu
Published: January 2020

Background

Cola nitida is commonly chewed in many West African cultures to ease hunger pangs and sometimes for their stimulant and euphoriant qualities. Metoclopramide is a known substrate for P-gp, SULT2A1 and CYP2D6 and studies have revealed that caffeine- a major component of Cola nitida can induce P-glycoprotein (P-gp), SULT2A1 and SULT1A1, hence a possible drug interaction may occur on co-administration. The aim of this study was to investigate the pharmacokinetic interactions of Cola nitida and metoclopramide in rabbits.

Methods

The study was performed in two stages using five healthy male rabbits with a 1-week washout period between treatments. Stage one involved oral administration of metoclopramide (0.5 mg/kg) alone while in the second stage, metoclopramide (0.5 mg/kg) was administered concurrently with Cola nitida (0.7 mg/kg). Blood samples were collected after each stage at predetermined intervals and analyzed for plasma metoclopramide concentration using HPLC.

Results

Compared with control, the metoclopramide/Cola nitida co-administration produced a decrease in plasma concentration of metoclopramide at all the time intervals except at the 7th hour. The following pharmacokinetic parameters were also decreased: area under the curve (51%), peak plasma concentration (39%), half-life (51%); while an increase in elimination rate constant (113%) and clearance rate (98%) were noted indicating rapid elimination of the drug. A minimal decrease in absorption rate (10%) was also observed.

Conclusions

The results of this study reveal a possible herb-drug interaction between Cola nitida and metoclopramide.
Broad consent under the GDPR: an optimistic perspective on a bright future
Authors: Hallinan Dara
Published: January 2020

Broad consent – the act of gaining one consent for multiple potential future research projects – sits at the core of much current genomic research practice. Since the 25th May 2018, the General Data Protection Regulation (GDPR) has applied as valid law concerning genomic research in the EU and now occupies a dominant position in the legal landscape. Yet, the position of the GDPR concerning broad consent has recently been cause for concern in the genomic research community. Whilst the text of the GDPR apparently supports the practice, recent jurisprudence contains language which is decidedly less positive. This article takes an in-depth look at the situation concerning broad consent under the GDPR and – despite the understandable concern flowing from recent jurisprudence – offers a positive outlook. This positive outlook is argued from three perspectives, each of which is significant in defining the current, and ongoing, legitimacy and utility of broad consent under the GDPR: the principled, the legal technical, and the practical.
Soil salinity assessment by using near-infrared channel and Vegetation Soil Salinity Index derived from Landsat 8 OLI data: a case study in the Tra Vinh Province, Mekong Delta, Vietnam
Authors: Nguyen Kim-Anh , Liou Yuei-An , Tran Ha-Phuong , Hoang Phi-Phung , Nguyen Thanh-Hung
Published: January 2020

Salinity intrusion is a pressing issue in the coastal areas worldwide. It affects the natural environment and causes massive economic loss due to its impacts on the agricultural productivity and food safety. Here, we assessed the salinity intrusion in the Tra Vinh Province, in the Mekong Delta of Vietnam. Landsat 8 OLI image was utilized to derive indices for soil salinity estimate including the single bands, Vegetation Soil Salinity Index (VSSI), Soil Adjusted Vegetation Index (SAVI), Normalized Difference Vegetation Index (NDVI), and Normalized Difference Salinity Index (NDSI). Statistical analysis between the electrical conductivity (EC1:5, dS/m) and the environmental indices derived from Landsat 8 OLI image was performed. Results indicated that spectral values of near-infrared (NIR) band and VSSI were better correlated with EC1:5(r2= 0.8 andr2= 0.7, respectively) than the other indices. Comparative results show that soil salinity derived from Landsat 8 was consistent with in situ data with coefficient of determination,R2= 0.89 and RMSE = 0.96 dS/m for NIR band andR2= 0.77 and RMSE = 1.27 dS/m for VSSI index. Findings of this study demonstrate that Landsat 8 OLI images reveal a high potential for spatiotemporally monitoring the magnitude of soil salinity at the top soil layer. Outcomes of this study are useful for agricultural activities, planners, and farmers by mapping the
soil salinitycontamination for better selection of accomodating crop types to reduce economical loss in the context of climate change. Our proposed method that estimates soil salinity using satellite-derived variables can be potentially useful as a fast-approach to detect the soil salinity in the other regions with low cost and considerable accuracy.
Application of wavelet for seismic wave analysis in Kathmandu Valley after the 2015 Gorkha earthquake, Nepal
Authors: Adhikari Binod , Dahal Subodh , Karki Monika , Mishra Roshan Kumar , Dahal Ranjan Kumar , Sasmal Sudipta , Klausner Virginia
Published: January 2020

In this paper, we estimate the seismogenic energy during the Nepal Earthquake (25 April 2015) and studied the ground motion time-frequency characteristics in Kathmandu valley. The idea to analyze time-frequency characteristic of seismogenic energy signal is based on wavelet transform which we employed here. Wavelet transform has been used as a powerful signal analysis tools in various fields like compression, time-frequency analysis, earthquake parameter determination, climate studies, etc. This technique is particularly suitable for non-stationary signal. It is well recognized that the earthquake ground motion is a non-stationary random process. In order to characterize a non-stationary random process, it is required immeasurable samples in the mathematical sense. The wavelet transformation procedures that we follow here helps in random analyses of linear and non-linear structural systems, which are subjected to earthquake ground motion. The manners of seismic ground motion are characterized through wavelet coefficients associated to these signals. Both continuous wavelet transform (CWT) and discrete wavelet transform (DWT) techniques are applied to study ground motion in Kathmandu Valley in horizontal and vertical directions. These techniques help to point out the long-period ground motion with site response. We found that the long-period ground motions have enough power for structural damage. Comparing both the horizontal and the vertical motion, we observed that the most of the high amplitude signals are associated with the vertical motion: the high energy is released in that direction. It is found that the seismic energy is damped soon after the main event; however the period of damping is different. This can be seen on DWT curve where square wavelet coefficient is high at the time of aftershock and the value decrease with time. In other words, it is mostly associated with the arrival of Rayleigh waves. We concluded that long-period ground motions should be studied by earthquake engineers in order to avoid structural damage during the earthquake. Hence, by using wavelet technique we can specify the vulnerability of seismically active region and local topological features out there.
Evaluation of landslide hazard and its impacts on hilly environment of the Nilgiris District - a geospatial approach
Authors: Thennavan Edison , Pattukandan Ganapathy Ganapathy
Published: January 2020

Landslide Hazard Zonation (LSH) maps play a key role in landuse planning particularly in landslide prone areas. LSH mapping is globally accepted one for analyzing the area for landslide susceptibility. Different approaches were followed by many researchers in India to prepare landslide hazard zonation mapping depending upon their need and requirement. The Nilgiris district in Western Ghats of India is one of the severe to high landslide hazard prone areas of India. Many agencies have carried out research on LSH mapping for the Nilgiris district with different scales. A systematic study of inventory and zonation was 122carried out in 1980’s by government agencies. However there is no proper updation or documentation on landslides after 1980’s in the district The purpose of this paper is to review the existing landslide-related studies in the district of The Nilgiris and review the district’s existing landslide hazard map with updated information. Landslide hazard maps in The Nilgiris were compiled in the GIS platform from various authenticated sources. Data on landslides from 1824 to 2014 were collected and a spatial database on landslides was created. A detailed inventory was analyzed and used for revision of the district’s landslide hazard impact on the 2009 landslides.. Based on the landslide inventory and densely populated areas and repeated landslides at the same locations, the most landslide hazard areas were identified.
‘Neo-Crosssexual’ fashion in contemporary men’s suits
Authors: Lee Hoe Ryung , Kim Jongsun , Ha Jisoo
Published: January 2020

Today, fashionable menswear is appropriating traditionally feminine design elements on an unparalleled international scale. This phenomenon should not be interpreted purely as a subversive gender issue, but should also be viewed as an expression of personal style and taste. In order to properly describe this phenomenon, the term ‘crosssexual’ must be introduced to English fashion vocabulary. This paper examines the innovative design characteristics of contemporary crosssexual menswear emerging prominently in men’s suits since 2015 and also raises the necessity of coining a new term: ‘neo-crosssexual’. Ultimately, this paper reveals how neo-crosssexual fashion employs a great variety of innovative silhouettes and novel design elements—ranging from structural or decorative details to colors, fabrics, patterns, and accessories—all of which enable the wearer to express whatever image of themselves they desire. Previous studies have interpreted crosssexual fashion as men simply portraying themselves as effeminate by wearing such clothing, but this conclusion is reductive and reveals there is still an unconscious recognition of limiting binary associations. The richness of neo-crosssexual fashion design, rather, gives individuals freedom to choose a sartorial image devoid of binary constraints. Conventionally classified ‘feminine designs’ are now perceived as creative and functional means to fluidity. This positive cultural shift has led to an increasing number of men choosing to wear suits that incorporate ‘feminine’ design elements, freeing this traditional garment from its limited formal use and symbolic hegemonic power.
Prostate cancer survivors’ preferences on the delivery of diet and lifestyle advice: a pilot best-worst discrete choice experiment
Authors: Robles Luke A. , Wright Stuart J. , Hackshaw-McGeagh Lucy , Shingler Ellie , Shiridzinomwa Constance , Lane J. Athene , Martin Richard M. , Burden Sorrel
Published: January 2020

Background

Lifestyle factors, including diet and physical activity, are associated with prostate cancer progression and mortality. However, it is unclear how men would like lifestyle information to be delivered following primary treatment. This study aimed to identify men’s preferences for receiving lifestyle information.

Methods

We conducted a cross-sectional pilot best-worst discrete choice experiment which was nested within a feasibility randomised controlled trial. Our aim was to explore men’s preferences of receiving diet and physical activity advice following surgery for localised prostate cancer. Thirty-eight men with a mean age of 65 years completed best-worst scenarios based on four attributes: (1) how information is provided; (2) where information is provided; (3) who provides information; and (4) the indirect cost of receiving information. Data was analysed using conditional logistic regression. Men’s willingness to pay (WTP) for aspects of the service was calculated using an out-of-pocket cost attribute.

Results

The combined best-worst analysis suggested that men preferred information through one-to-one discussion β = 1.07, CI = 0.88 to 1.26) and not by email (β = − 1.02, CI = − 1.23 to − 0.80). They preferred information provided by specialist nurses followed by dietitians (β = 0.76, CI = 0.63 to 0.90 and − 0.16, CI = − 0.27 to − 0.05 respectively) then general nurses (β = − 0.60, CI = − 0.73 to − 0.48). Three groups were identified based on their preferences. The largest group preferred information through individual face-to-face or group discussions (β = 1.35, CI = 1.05 to 1.63 and 0.70, CI = 0.38 to 1.03 respectively). The second group wanted information via one-to-one discussions or telephone calls (β = 1.89, CI = 1.41 to 2.37 and 1.03, CI = 0.58 to 1.48 respectively), and did not want information at community centres (β = − 0.50, CI = − 0.88 to − 0.13). The final group preferred individual face-to-face discussions (β = 0.45, CI = 0.03 to 0.88) but had a lower WTP value (£17).

Conclusions

Men mostly valued personalised methods of receiving diet and physical activity information over impersonal methods. The out-of-pocket value of receiving lifestyle information was important to some men. These findings could help inform future interventions using tailored dietary and physical activity advice given to men by clinicians following treatment for prostate cancer, such as mode of delivery, context, and person delivering the intervention. Future studies should consider using discrete choice experiments to examine information delivery to cancer survivor populations.