bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations between Taste and Smell Sensitivity, Preference and Quality of Life in Healthy Aging—The NutriAct Family Study Examinations (NFSE) Cohort
Authors: Lim Shirley X. L. , Höchenberger Richard , Busch Niko A. , Bergmann Manuela , Ohla Kathrin
Published: March 2022

Taste and smell function decline with age, with robust impairment in the very old. Much less is known about taste and smell function in young and middle aged. We investigated taste and smell sensitivity via thresholds in a sub-sample of the NutriAct Family Study (NFS), the NFS Examinations cohort (NFSE; N = 251, age M = 62.5 years). We examined different aspects relating to taste and smell function: the degree to which taste and smell sensitivity relate to another and to taste and smell preferences, the role of gender and age, as well as effects on Quality of Life (QoL). Taste thresholds were highly correlated, but no correlation was observed between taste and smell thresholds and between thresholds and preference. Women were more sensitive for both taste and smell than men. We found no effect of age on sensitivity and no effect of sensitivity on QoL. All null findings were complemented by Bayesian statistics. Together our results indicate the independence of taste and smell despite their overlap during sensorial experiences. We found no evidence for age-related sensory decline, which could be due to our sample’s characteristics of non-clinical volunteers with good dental health and 93% non-smokers.
Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio
Authors: Rodríguez-Pérez María Dolores , Pérez de Algaba Inmaculada , Martín-Aurioles Esther , Arrebola María Monsalud , Ortega-Hombrados Laura , Verdugo Cristina , Fernández-Prior María África , Bermúdez-Oria Alejandra , De La Cruz José Pedro , González-Correa José Antonio
Published: March 2022

The aim of this study was to assess the possible neuroprotective effect of 3′,4′-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). The neuroprotective effect was assessed in a hypoxia–reoxygenation model in brain slices and by quantifying retinal nerve cells. The animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic rats (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DR treated with DHPG (0.5 mg/kg/day), or (5) with 1 mg/kg/day, (6) DR treated with HT plus DHPG 0.5 mg/kg/day, or (7) HT plus 1 mg/kg/day p.o. DHPG. Diabetic animals presented higher levels of oxidative stress variables and lower numbers of neuronal cells in retinal tissue. The administration of DHPG or HT reduced most of the oxidative stress variables and brain lactate dehydrogenase efflux (LDH) as an indirect index of cellular death and also reduced the loss of retinal cells. The association of DHPG+HT in the same proportions, as found in EVOO, improved the neuroprotective and antioxidant effects of both polyphenols.
Dietary Acid Load Modulation of Asthma-Related miRNAs in the Exhaled Breath Condensate of Children
Authors: Mendes Francisca Castro , Paciência Inês , Cavaleiro Rufo João , Silva Diana , Delgado Luís , Moreira André , Moreira Pedro
Published: March 2022

Individual nutrients and bioactive compounds have been implicated in the expression of microRNAs (miRNAs), which are related to inflammation and asthma. However, evidence about the impact of diet is scarce. Therefore, we aimed to assess the association between dietary acid load and asthma-related miRNA in the exhaled breath condensate (EBC) of school-aged children. This cross-sectional analysis included 150 participants aged 7 to 12 years (52% girls) from a nested case–control study, which randomly selected 186 children attending 71 classrooms from 20 public schools located in city of Porto, Portugal. Dietary data were collected by one 24 h-recall questionnaire. Dietary acid load was assessed using the potential renal acid load (PRAL) and net endogenous acid production (NEAP) scores. Based on previous studies, eleven asthma-related miRNAs were chosen and analyzed in EBC by reverse transcription-quantitative real-time PCR. PRAL, NEAP and miRNAs were categorized as high or low according to the median. Logistic regression models were performed to assess the association between dietary acid load scores and miRNAs. Children in high dietary acid load groups (PRAL ≥ 14.43 and NEAP ≥ 55.79 mEq/day) have significantly increased odds of having high miR-133a-3p levels. In conclusion, higher dietary acid loads possibly modulate asthma-related miRNAs of school-aged children.
Therapeutic Efficacy of Orally Administered Nitrofurantoin against Animal African Trypanosomosis Caused by Trypanosoma congolense Infection
Authors: Suganuma Keisuke , N’Da David D. , Watanabe Ken-ichi , Tanaka Yusuke , Mossaad Ehab , Elata Afraa , Inoue Noboru , Kawazu Shin-ichiro
Published: March 2022

Animal African trypanosomosis (AAT) leads to emaciation and low productivity in infected animals. Only six drugs are commercially available against AAT; they have severe side effects and face parasite resistance. Thus, the development of novel trypanocidal drugs is urgently needed. Nitrofurantoin, an antimicrobial, is used for treating bacterial urinary tract infections. Recently, we reported the trypanocidal effects of nitrofurantoin and its analogs in vitro. Furthermore, a nitrofurantoin analog, nifurtimox, is currently used to treat Chagas disease and chronic human African trypanosomiasis. Thus, this study was aimed at evaluating the in vivo efficacy of nitrofurantoin in treating AAT caused by Trypanosoma congolense. Nitrofurantoin was orally administered for 7 consecutive days from 4 days post-infection in T. congolense-infected mice, and the animals were observed for 28 days. Compared to the control group, the treatment group showed significantly suppressed parasitemia at 6 days post-infection. Furthermore, survival was significantly prolonged in the group treated with at least 10 mg/kg nitrofurantoin. Moreover, 100% survival and cure was achieved with a dose of nitrofurantoin higher than 30 mg/kg. Thus, oral nitrofurantoin administration has potential trypanocidal efficacy against T. congolense-induced AAT. This preliminary data will serve as a benchmark when comparing future nitrofurantoin-related compounds, which can overcome the significant shortcomings of nitrofurantoin that preclude its viable use in livestock.
Reducing the Bitter Taste of Pharmaceuticals Using Cell-Based Identification of Bitter-Masking Compounds
Authors: Beltrán Leopoldo Raul , Sterneder Sonja , Hasural Ayse , Paetz Susanne , Hans Joachim , Ley Jakob Peter , Somoza Veronika
Published: March 2022

The palatability of a pharmaceutical preparation is a significant obstacle in developing a patient-friendly dosage form. Bitter taste is an important factor for patients in (i) selecting a certain drug from generic products available in the market and (ii) adhering to a therapeutic regimen. The various methods developed for identification of bitter tasting and bitter-taste modulating compounds present a number of limitations, ranging from limited sensitivity to lack of close correlations with sensory data. In this study, we demonstrate a fluorescence-based assay, analyzing the bitter receptor TAS2R-linked intracellular pH (pHi) of human gastric parietal (HGT-1) cells as a suitable tool for the identification of bitter tasting and bitter-taste modulating pharmaceutical compounds and preparations, which resembles bitter taste perception. Among the fluorometric protocols established to analyze pHi changes, one of the most commonly employed assays is based on the use of the pH-sensitive dye SNARF-1 AM. This methodology presents some limitations; over time, the assay shows a relatively low signal amplitude and sensitivity. Here, the SNARF-1 AM methodology was optimized. The identified bicarbonate extrusion mechanisms were partially inhibited, and measurements were carried out in a medium with lower intrinsic fluorescence, with no need for controlling external CO2 levels. We applied the assay for the screening of flavonoids as potential bitter-masking compounds for guaifenesin, a bitter-tasting antitussive drug. Our findings revealed that eriodictyol, hesperitin and phyllodulcin were the most potent suitable candidates for bitter-masking activity, verified in a human sensory trial.
Novel Fluticasone Propionate and Salmeterol Fixed-Dose Combination Nano-Encapsulated Particles Using Polyamide Based on L-Lysine
Authors: Alyami Mohammad H. , Dahmash Eman Zmaily , Ali Dalia Khalil , Alyami Hamad S. , AbdulKarim Hussien , Alsudir Samar A.
Published: March 2022

One of the key challenges in developing a dry powder inhaler (DPI) of an inhalable potent fixed-dose combination (FDC) is the ability of the formulation to generate an effective and reproducible aerosol able to reach the lower parts of the lungs. Herein, a one-step approach is presented to expedite the synthesis of nanoaggregates made from a biocompatible and biodegradable polyamide based on L-lysine amino acid employing market-leading active pharmaceutical ingredients (fluticasone propionate (FP) and salmeterol xinafoate (SAL)) for the management of asthma. The nanoaggregates were synthesized using interfacial polycondensation that produced nanocapsules with an average particle size of 226.7 ± 35.3 nm and zeta potential of −30.6 ± 4.2 mV. Differential scanning calorimetric analysis and x-ray diffraction, as well as scanning electron microscopy of the produced FDC, revealed the ability of the produced nanocapsules to encapsulate the two actives and display the best aerodynamic performance. The FDC nanocapsules displayed 88.5% and 98.5% of the emitted dose for FP and SAL, respectively. The fine particle fraction of the nominated dose was superior to the marketed product (Seretide Diskus®, Brentford, United Kingdom). The in-vitro release study showed an extended drug release profile. Our findings suggest that nanoaggregates using polyamides based on L-lysine and interfacial polycondensation can serve as a good platform for pulmonary drug delivery of FDC systems.
Traditional and Computational Screening of Non-Toxic Peptides and Approaches to Improving Selectivity
Authors: Robles-Loaiza Alberto A. , Pinos-Tamayo Edgar A. , Mendes Bruno , Ortega-Pila Josselyn A. , Proaño-Bolaños Carolina , Plisson Fabien , Teixeira Cátia , Gomes Paula , Almeida José R.
Published: March 2022

Peptides have positively impacted the pharmaceutical industry as drugs, biomarkers, or diagnostic tools of high therapeutic value. However, only a handful have progressed to the market. Toxicity is one of the main obstacles to translating peptides into clinics. Hemolysis or hemotoxicity, the principal source of toxicity, is a natural or disease-induced event leading to the death of vital red blood cells. Initial screenings for toxicity have been widely evaluated using erythrocytes as the gold standard. More recently, many online databases filled with peptide sequences and their biological meta-data have paved the way toward hemolysis prediction using user-friendly, fast-access machine learning-driven programs. This review details the growing contributions of in silico approaches developed in the last decade for the large-scale prediction of erythrocyte lysis induced by peptides. After an overview of the pharmaceutical landscape of peptide therapeutics, we highlighted the relevance of early hemolysis studies in drug development. We emphasized the computational models and algorithms used to this end in light of historical and recent findings in this promising field. We benchmarked seven predictors using peptides from different data sets, having 7–35 amino acids in length. According to our predictions, the models have scored an accuracy over 50.42% and a minimal Matthew’s correlation coefficient over 0.11. The maximum values for these statistical parameters achieved 100.0% and 1.00, respectively. Finally, strategies for optimizing peptide selectivity were described, as well as prospects for future investigations. The development of in silico predictive approaches to peptide toxicity has just started, but their important contributions clearly demonstrate their potential for peptide science and computer-aided drug design. Methodology refinement and increasing use will motivate the timely and accurate in silico identification of selective, non-toxic peptide therapeutics.
The Phenomenon of Drug Emulsion Carriers Compaction during Their Movement in Microstructures
Authors: Błaszczyk Mariola M. , Sęk Jerzy , Przybysz Łukasz
Published: March 2022

The greatest challenges of modern pharmacology are the design of drugs with the highest possible efficacy of an active substance and with the lowest possible invasiveness for the whole organism. A good solution features the application of a bioactive substance in different carriers. The effectiveness of such preparations is determined not only by the properties of the drug, but primarily by the dynamics of carrier movement in the body. This is the reason why studies on the dispersed systems transport in micro- and nanostructures are becoming important. This paper presents a study of emulsion systems transport in microcapillaries. A dispersed phase thickening effect was observed during the process, which resulted in a concentration increase of the flowing emulsion, in some cases up to 10 times. This phenomenon directly influences transport dynamics of such substances in microstructures and should be taken into account when designing drug parameters (concentration, release time, and action range). The effect was investigated for three different emulsions concentrations and presented quantitatively. The scales of this phenomenon occurrence at different flow conditions were investigated, and their magnitudes were modelled and described. This allows the prediction of the flow resistance in the movement of given dispersion systems, as a function of the flow rate, the emulsion parameters, and the microchannel size.
The Geriatric Virtual Escape Room in Pharmacy Education: Female Students Escape Significantly Faster than Male Students
Authors: Badr Aisha F.
Published: March 2022

Due to COVID-19 and the limitation of face-face teaching, electronic adaptation for formative and continuous assessment methods were greatly used and documented between 2020 and 2021. This study aims to implement a virtual escape room that will help assist and refine problem-solving skills in fifth-year pharmacy students by reviewing Beer’s criteria and selecting the most appropriate management. A descriptive cross-sectional study was conducted following the implementation of the virtual escape room using google form. Students had to unlock five puzzles using Beer’s criteria. To evaluate pharmacy students’ perception of this method, they completed a survey to identify their views of the game. Of the 128 students enrolled in the geriatric course, all were able to escape (100%). A one-sample t-test indicated statistical significance between gender. Female students escaped statistically faster than male students (p < 0.00002) and were more likely to recommend the game to other students and thought the game encouraged them to think of the material in a new way, whereas male students were more neutral towards it. In conclusion, the geriatric virtual escape room was successfully implemented as a pilot innovative method to assist in virtual learning. However, future studies should investigate virtual gamification in pharmacy education and its impact on learning, as well as identify if there were any gender-specific differences in using these tools.
Multidisciplinarity, Interdisciplinarity, and Transdisciplinarity: The Tower of Babel in the Age of Two Cultures
Authors: Schroeder Marcin J.
Published: March 2022

Despite the continuous emphasis on globalization, we witness increasing divisions and divisiveness in all domains of human activities. One of the reasons, if not the main one, is the intellectual fragmentation of humanity, compared in the title to the failed attempt at building the Biblical Tower of Babel. The attempts to reintegrate worldview, fragmented by the specialization of education (C.P. Snow’s The Two Cultures) and expected to be achieved through reforms in curricula at all levels of education, were based on the assumption that the design of a curriculum should focus on the wide distribution of subjects of study, as if the distribution was the goal. The key point is not the distribution of themes, but the development of skills in the integration of knowledge. The quantitative assessment of the width of knowledge by the number of disciplines is of secondary importance. We cannot expect the miracle that students without any intellectual tools developed for this purpose would perform the job of integration, which their teachers do not promote or demonstrate, and which they cannot achieve for themselves. There are many other reasons for the increasing interest in making inquiries interdisciplinary, but there is little progress in the methodology of the integration of knowledge. This paper is a study of the transition from multidisciplinarity to interdisciplinarity, and further, to transdisciplinarity, with some suggestions regarding the use of methodological tools of structuralism and the choice of a conceptual framework.
Inconsistent, Vague, and…Just? An Analysis of the National Football League’s 2021 COVID-19 Policy
Authors: Gimbel Steven , Radzevick Joseph
Published: March 2022

The National Football League, the premier professional organization for American football, developed a policy concerning the protocol in cases where players contract COVID-19. This policy includes elements such as collective punishment that appear, at first glance, to be morally problematic. To the contrary, the policy is indeed morally acceptable as we should not think of organizations such as the NFL in the same way we think of governments in stable nations, but rather in the same way that we think of hybrid justice systems in countries where because of histories of colonialism, the identity of citizens is divided, with tribal identities being more important than national citizenship.
Mitigation of Gastric Damage Using Cinnamomum cassia Extract: Network Pharmacological Analysis of Active Compounds and Protection Effects in Rats
Authors: Lee Ji Hwan , Kwak Hee Jae , Shin Dongchul , Seo Hye Jin , Park Shin Jung , Hong Bo-Hee , Shin Myoung-Sook , Kim Seung Hyun , Kang Ki Sung
Published: March 2022

Gastritis is a common disease worldwide that is caused by various causes such as eating habits, smoking, severe stress, and heavy drinking, as well as Helicobacter pylori infections and non-steroidal anti-inflammatory drugs. Cinnamomum cassia is a tropical aromatic evergreen tree commonly used as a natural medicine in Asia and as a functional food ingredient. Studies have reported this species’ anti-obesity, anti-diabetic, and cardiovascular disease suppression effects. We evaluated the potential effects of C. cassia using non-steroidal anti-inflammatory drugs (NSAIDs), ethanol (EtOH), and ethanol/hydrochloric acid (HCl)-induced gastric mucosal injury models. C. cassia extracts reduced the area of gastric mucosa injury caused by indomethacin, NSAID, EtOH, and EtOH/HCl. We also applied a network pharmacology-based approach to identify the active compounds, potential targets, and pharmacological mechanisms of C. cassia against gastritis. Through a network pharmacology analysis, 10 key components were predicted as anti-gastritis effect-related compounds of C. cassia among 51 expected active compounds. The NF-κB signaling pathway, a widely known inflammatory response mechanism, comprised a major signaling pathway within the network pharmacology analysis. These results suggest that the anti-gastritis activities of C. cassia may be induced via the anti-inflammatory effects of key components, which suppress the inflammation-related genes and signaling pathways identified in this study.
Vascular Plants Flora of Mire Ecosystem of the Bolshoy Shantar Island (the Far East of Russia)
Authors: Kuptsova Viktoriya A. , Antonova Lyubov A. , Chakov Vladimir V.
Published: March 2022

This article presents the findings of the authors’ study of the mire ecosystem vascular plants of the island of Bolshoy Shantar, which is the largest island in the Shantar archipelago. Bolshoy Shantar Island is an insular ecosystem, the study of which can provide insight into the natural “polygons” of evolution at work. The botanical research was conducted through the application of traditional techniques of floristic and geobotanical studies. The material for this article was drawn from 73 floristic and 54 geobotanical descriptions made between 2016–2018 in the north-eastern part of Bolshoy Shantar Island on four mire massifs associated with various hypsometric surfaces. The findings of this study indicate that the flora of vascular plants of the mires of Bolshoy Shantar Island reflect the peculiarities of a regional mire type that formed in the insular conditions of the Pacific. The species richness of the vascular flora of the island’s mire ecosystems is evidenced by a total species count of 158, composed of 109 genera and 48 families, which accounts for more than one quarter (26.3%) of the Shantar archipelago’s flora. Over half of these species (63.7%) form the core of the mire flora.
Ecophysiological Leaf Traits of Forty-Seven Woody Species under Long-Term Acclimation in a Botanical Garden
Authors: Sun Qinglin , Lai Liming , Zhou Jihua , Liu Xin , Zheng Yuanrun
Published: March 2022

Ex situ conservation plays an important role in the conservation and utilization of plant resources. In recent years, botanical gardens have greatly improved the ex situ conservation of plants, and research has mainly focused on morphological characteristics, reproduction technology, and conservation value. There are few studies on the ecophysiological traits of plants after conservation. Forty-seven plants that are frequently used in North China and were grown in the Beijing Botanic Garden were selected to measure their photosynthetic traits, light-use efficiency (LUE), water–use efficiency (WUE), specific leaf area (SLA), relative chlorophyll content (SPAD), and leaf water potential (φ). An analysis of variance showed that there were significant differences in the ecophysiological traits of the leaves of 47 woody species. The light saturation point (LSP), net photosynthetic rate at light saturation (Pnmax), φ, and SLA had significant differences among different plant life forms. The SLA and SPAD of leaves were significantly different among the families. The LUE of all species reached its maximum under a low light intensity, and species with a large difference between the light saturation point and light compensation point had larger Pnmax values. This research further adds to the understanding of the adaptation mechanisms of plants to the environment under the conditions of a botanical garden as well as the environmental fitness in a long-term ex situ domestication and then helps with scientifically setting up artificial management conditions.
Preparation of Needleless Electrospinning Polyvinyl Alcohol/Water-Soluble Chitosan Nanofibrous Membranes: Antibacterial Property and Filter Efficiency
Authors: Lou Ching-Wen , Lin Meng-Chen , Huang Chen-Hung , Lai Mei-Feng , Shiu Bing-Chiuan , Lin Jia-Horng
Published: March 2022

Electrospinning is an efficient method of producing nanofibers out of polymers that shows a great potential for the filtration territory. Featuring water-soluble chitosan (WS-CS), a low-pollution process and a self-made needleless machine, PVA/WS-CS nanofibrous membranes were prepared and evaluated for nanofiber diameter, bacteriostatic property, filtration efficiency, pressure drop, and quality factor. Test results indicate that the minimal fiber diameter was 216.58 ± 58.15 nm. Regardless of the WS-CS concentration, all of the PVA/WS-CS nanofibrous membranes attained a high porosity and a high water vapor transmission rate (WVTR), with a pore size of 12.06–22.48 nm. Moreover, the membranes also exhibit bacteriostatic efficacy against Staphylococcus aureus, an optimal quality factor of 0.0825 Pa−1, and a filtration efficiency as high as 97.0%, that is 72.5% higher than that of common masks.
Spectroscopic Ellipsometry and Quartz Crystal Microbalance with Dissipation for the Assessment of Polymer Layers and for the Application in Biosensing
Authors: Plikusiene Ieva , Maciulis Vincentas , Ramanavicius Arunas , Ramanaviciene Almira
Published: March 2022

Polymers represent materials that are applied in almost all areas of modern life, therefore, the characterization of polymer layers using different methods is of great importance. In this review, the main attention is dedicated to the non-invasive and label-free optical and acoustic methods, namely spectroscopic ellipsometry (SE) and quartz crystal microbalance with dissipation (QCM-D). The specific advantages of these techniques applied for in situ monitoring of polymer layer formation and characterization, biomolecule immobilization, and registration of specific interactions were summarized and discussed. In addition, the exceptional benefits and future perspectives of combined spectroscopic ellipsometry and QCM-D (SE/QCM-D) in one measurement are overviewed. Recent advances in the discussed area allow us to conclude that especially significant breakthroughs are foreseen in the complementary application of both QCM-D and SE techniques for the investigation of polymer structure and assessment of the interaction between biomolecules such as antigens and antibodies, receptors and ligands, and complementary DNA strands.
Birefringence of Thin Uniaxial Polymer Films Estimated Using the Light Polarization Ellipse
Authors: Postolache Mihai , Dimitriu Dan Gheorghe , Nechifor Cristina Delia , Condurache Bota Simona , Closca Valentina , Dorohoi Dana Ortansa
Published: March 2022

A simple method for determining the linear birefringence of the thin layers based on the determination of the orientation of the polarization ellipse of totally polarized light is proposed and it is applied to PVA thin foils. Theoretical notions and the experimental procedure are described. The linear birefringence of polymer thin foils with different degrees of stretching is determined and the applicability of the method is discussed.
Comparison of Protein Content, Availability, and Different Properties of Plant Protein Sources with Their Application in Packaging
Authors: Senthilkumaran Anupriya , Babaei-Ghazvini Amin , Nickerson Michael T. , Acharya Bishnu
Published: March 2022

Plant-based proteins are considered to be one of the most promising biodegradable polymers for green packaging materials. Despite this, the practical application of the proteins in the packaging industry on a large scale has yet to be achieved. In the following review, most of the data about plant protein-based packaging materials are presented in two parts. Firstly, the crude protein content of oilseed cakes and meals, cereals, legumes, vegetable waste, fruit waste, and cover crops are indexed, along with the top global producers. In the second part, we present the different production techniques (casting, extrusion, and molding), as well as compositional parameters for the production of bioplastics from the best protein sources including sesame, mung, lentil, pea, soy, peanut, rapeseed, wheat, corn, amaranth, sunflower, rice, sorghum, and cottonseed. The inclusion of these protein sources in packaging applications is also evaluated based on their various properties such as barrier, thermal, and mechanical properties, solubility, surface hydrophobicity, water uptake capacity, and advantages. Having this information could assist the readers in exercising judgement regarding the right source when approving the applications of these proteins as biodegradable packaging material.
The Distribution and Polymerization Mechanism of Polyfurfuryl Alcohol (PFA) with Lignin in Furfurylated Wood
Authors: Xu Jindi , Hu Dongying , Zheng Qi , Meng Qiulu , Li Ning
Published: March 2022

There is increasing interest in furfurylated wood, but the polymerization mechanism between its internal polyfurfuryl alcohol (PFA) and lignin is still uncertain. This paper investigated the distribution of PFA and the feasibility of the polymerization of PFA with lignin in furfurylated balsa wood. The wood first immersed in the furfuryl alcohol (FA) solution followed by in situ polymerization and the distribution of PFA was characterized by Raman, fluorescence microscopy, SEM, and CLSM. Then, the mill wood lignin (MWL) of balsa wood and lignin model molecules were catalytically polymerized with PFA, respectively, studying the mechanism of interaction between PFA and lignin. It was concluded that PFA was mainly deposited in cell corner with high lignin concentration, and additionally partly deposited in wood cell cavity due to high concentration of FA and partial delignification. TGA, FTIR, and NMR analysis showed that the cross-linked network structure generated by the substitution of MWL aromatic ring free position by PFA hydroxymethyl enhanced the thermal stability. New chemical shifts were established between PFA and C5/C6 of lignin model A and C2/C6 of model B, respectively. The above results illustrated that lignin-CH2-PFA linkage was created between PFA and lignin in the wood cell wall.
Novel Thymoquinone Nanoparticles Using Poly(ester amide) Based on L-Arginine-Targeting Pulmonary Drug Delivery
Authors: Dahmash Eman Zmaily , Ali Dalia Khalil , Alyami Hamad S. , AbdulKarim Hussien , Alyami Mohammad H. , Aodah Alhassan H.
Published: March 2022

Thymoquinone (TQ), the main active constituent of Nigella sativa, has demonstrated broad-spectrum antimicrobial, antioxidant, and anti-inflammatory effects, which suggest its potential use in secondary infections caused by COVID-19. However, clinical deployment has been hindered due to its limited aqueous solubility and poor bioavailability. Therefore, a targeted delivery system to the lungs using nanotechnology is needed to overcome limitations encountered with TQ. In this project, a novel TQ-loaded poly(ester amide) based on L-arginine nanoparticles was prepared using the interfacial polycondensation method for a dry powder inhaler targeting delivery of TQ to the lungs. The nanoparticles were characterized by FTIR and NMR to confirm the structure. Transmission electron microscopy and Zetasizer results confirmed the particle diameter of 52 nm. The high-dose formulation showed the entrapment efficiency and loading capacity values of TQ to be 99.77% and 35.56%, respectively. An XRD study proved that TQ did not change its crystallinity, which was further confirmed by the DSC study. Optimized nanoparticles were evaluated for their in vitro aerodynamic performance, which demonstrated an effective delivery of 22.7–23.7% of the nominal dose into the lower parts of the lungs. The high drug-targeting potential and efficiency demonstrates the significant role of the TQ nanoparticles for potential application in COVID-19 and other respiratory conditions.
Chemical Recycling of Vacuum-Infused Thermoplastic Acrylate-Based Composites Reinforced by Basalt Fabrics
Authors: Meyer zu Reckendorf Inès , Sahki Amel , Perrin Didier , Lacoste Clément , Bergeret Anne , Ohayon Avigaël , Morand Karynn
Published: March 2022

The objective of this work was to compare the material recovered from different chemical recycling methodologies for thermoplastic acrylate-based composites reinforced by basalt fabrics and manufactured by vacuum infusion. Recycling was done via chemical dissolution with a preselected adapted solvent. The main goal of the study was to recover undamaged basalt fabrics in order to reuse them as reinforcements for “second-generation” composites. Two protocols were compared. The first one is based on an ultrasound technique, the second one on mechanical stirring. Dissolution kinetics as well as residual resin percentages were evaluated. Several parameters such as dissolution duration, dissolution temperature, and solvent/composite ratio were also studied. Recycled fabrics were characterized through SEM observations. Mechanical and thermomechanical properties of second-generation composites were determined and compared to those of virgin composites (called “first-generation” composites). The results show that the dissolution protocol using a mechanical stirring is more adapted to recover undamaged fabrics with no residual resin on their surface. Moreover, corresponding second-generation composites display equivalent mechanical properties than first generation ones.
Insights into the Impact of Rosmarinic Acid on CHO Cell Culture Improvement through Transcriptomics Analysis
Authors: Huang Zhuangrong , Xu Jianlin , Tian Jun , Aron Kathryn , Qian Yueming , Borys Michael , Li Zhengjian , Pendse Girish
Published: March 2022

The use of antioxidants in Chinese hamster ovary (CHO) cell cultures to improve monoclonal antibody production has been a topic of great interest. Nevertheless, the antioxidants do not have consistent benefits of production improvement, which might be cell line specific and/or process specific. In this work, we investigated how treatment with the antioxidant rosmarinic acid (RA) improved cell growth and titer in CHO cell cultures using transcriptomics. In particular, transcriptomics analysis indicated that RA treatment modified gene expression and strongly affected the MAPK and PI3K/Akt signaling pathways, which regulate cell survival and cell death. Moreover, it was observed that these signaling pathways, which had been identified to be up-regulated on day 2 and day 6 by RA, were also up-regulated over time (from initial growth phase day 2 to slow growth or protein production phase day 6) in both conditions. In summary, this transcriptomics analysis provides insights into the role of the antioxidant RA in industrial cell culture processes. The current study also represents an example in the industry of how omics can be applied to gain an in-depth understanding of CHO cell biology and to identify critical pathways that can contribute to cell culture process improvement and cell line engineering.
Prediction of Heavy Metal Concentrations in Contaminated Sites from Portable X-ray Fluorescence Spectrometer Data Using Machine Learning
Authors: Xia Feiyang , Fan Tingting , Chen Yun , Ding Da , Wei Jing , Jiang Dengdeng , Deng Shaopo
Published: March 2022

Portable X-ray fluorescence (pXRF) spectrometers provide simple, rapid, nondestructive, and cost-effective analysis of the metal contents in soils. The current method for improving pXRF measurement accuracy is soil sample preparation, which inevitably consumes significant amounts of time. To eliminate the influence of sample preparation on PXRF measurements, this study evaluates the performance of pXRF measurements in the prediction of eight heavy metals’ contents through machine learning algorithm linear regression (LR) and multivariate adaptive regression spline (MARS) models. Soil samples were collected from five industrial sites and separated into high-value and low-value datasets with pXRF measurements above or below the background values. The results showed that for Cu and Cr, the MARS models were better than the LR models at prediction (the MARS-R2 values were 0.88 and 0.78; the MARS-RPD values were 2.89 and 2.11). For the pXRF low-value dataset, the multivariate MARS models improved the pXRF measurement accuracy, with the R2 values improved from 0.032 to 0.39 and the RPD values increased by 0.02 to 0.37. For the pXRF high-value dataset, the univariate MARS models predicted the content of Cu and Cr with less calculation. Our study reveals that machine learning methods can better predict the Cu and Cr of large samples from multiple contaminated sites.
Contextual, Taphonomic, and Paleoecological Insights from Anurans on Tiwanaku Sites in Southern Peru
Authors: Rubinatto Serrano Juliana , Vallejo-Pareja Maria Camila , deFrance Susan D. , Baitzel Sarah I. , Goldstein Paul S.
Published: March 2022

We examine the processes that resulted in the deposition of bones of at least three anuran genera on four archaeological sites associated with the Tiwanaku culture occupied between 700–1100 CE in the Moquegua Valley of far southern Peru. We review archaeological data and ethnographic accounts of Andean peoples using frogs and toads for food and in rain-quest rituals. Anuran bones are common in prehispanic cemeteries, but far less common in habitational and ceremonial sites. The quantity of anuran remains in certain cemeteries is anomalous in comparison to other archaeological sites in the valley and to Tiwanaku sites in other geographic settings. We conclude that anurans are most common where abandoned below-ground rock-covered tombs are likely to have been reused by nesting owls, and propose that most anuran remains in archaeological contexts in Moquegua are the result of predation. We consider environmental, cultural and taphonomic explanations and posit that the abundance of anuran remains relates to the 14th-century Miraflores ENSO event. This event generated increased rainfall in the desert, creating conditions favorable for frogs and toads, and predation by owls. We also advocate for the use of fine-screening to recover small-sized animal remains, such as anurans, that can be used to understand taphonomic processes and paleoenvironmental conditions.
Interreligious Dialogue and Pilgrimage: The Case of the Tamil Community in Palermo
Authors: Salerno Rossana M.
Published: March 2022

This ethnographic research on the territory of Palermo is a comparison between the old immigrant Tamil generations, coming from Sri Lanka, and the new, native residents of Palermo. There are two points of generational convergence in the community: the cult during the festive rituals of Santa Rosalia, and the territorial symbolism of Mount Pellegrino. This peculiarity, qualitatively analysed through the NVivo software, is revealed by two specific and organized events: the first by the archbishop’s curia and the second by the community itself. The first consists of the participation in the day dedicated to interreligious dialogue (from 2014 until today), and the second is the night of the “acchianata” (ascent) on 3 and 4 September. The day dedicated to “interreligious dialogue” involves the meeting of all confessions/religious communities in Palermo, creating an annual meeting ritual under the sign of Santa Rosalia.
The Bestowal of Noble Titles upon the Mountain and Water Spirits in Tang China
Authors: Zhu Yi
Published: March 2022

In the Tang era, official sacrifices to mountain and water spirits became more methodical than those of the preceding dynasties. What deserves more attention is that the imperial court bestowed noble titles, which were normally awarded to aristocrats and powerful officials, on the twenty-eight mountain and water spirits, including the Five Sacred Peaks, Four Strongholds, Four Seas and Four Waterways. These practices reflected the two-sided attitude of the Tang rulers to the mountain and water spirits. When confronting violent political changes, the rulers yearned for blessings and protection from these natural deities. On the other hand, with the expansion of monarchical power in the secular world, they sought to establish their authority in the realm of divinity. Running parallel in most cases, the bestowal of nobility and the official sacrificial system constituted the official cult of mountain and water spirits, which survived until the first years of the Ming Dynasty.
Sweet Asceticism: An Ethnographic Study of Female Renouncers in the Chaitanya Vaiṣṇava Tradition
Authors: Taneja Leena
Published: March 2022

This paper is based on an ethnographic study which aims to examine female asceticism in the Chaitanya Vaishnava sect, a Hindu devotional school found in the region of Vrindavan in Northwest India. Asceticism, meaning to renounce worldly life, is deeply rooted in Hindu practice. Yet, despite its wide acceptance, female asceticism has remained on the margins of Hindu religious experience. Despite the lack of a model of asceticism for women, scripturally and sociologically, female ascetics are a growing religious group in India. This paper seeks to use empirical data collected during two years of fieldwork to examine how asceticism is articulated and performed by women living in Vrindavan. It builds upon recent interventions in key areas of feminist scholarship and asceticism in South Asia by engaging a religious sect which has received little attention by feminist scholars. This engagement, it is believed, can productively enlarge the field of feminist theologizing and South Asian asceticism.
A New Spatial Filtering Algorithm for Noisy and Missing GNSS Position Time Series Using Weighted Expectation Maximization Principal Component Analysis: A Case Study for Regional GNSS Network in Xinjiang Province
Authors: Li Wudong , Li Zhao , Jiang Weiping , Chen Qusen , Zhu Guangbin , Wang Jian
Published: March 2022

Common Mode Error (CME) presents a kind of spatially correlated error that is widespread in regional Global Navigation Satellite System (GNSS) networks and should be eliminated during postprocessing of a GNSS position time series. Several spatiotemporal filtering methods have been developed to mitigate the effects of CME. However, such methodologies become inappropriate when missing and noisy data exists. In this research, we introduce a novel spatial filtering algorithm called Weighted Expectation Maximization Principal Component Analysis (WEMPCA) for detecting and removing CME from noisy GNSS position time series with missing values, among which formal errors of daily GNSS solutions are utilized to weight the input data. Compared with traditional PCA and the special case of EMPCA, simulation experiments demonstrate that the new WEMPCA algorithm always has outstanding performance over others. The WEMPCA algorithm was then successfully used to extract the CME from real noisy and missing GNSS position time series in Xinjiang province. Our results show that only the first principal component exhibits significant spatial response, with average values of 70.11%, 66.53%, and 52.45% for North, East, and Up (NEU) components, respectively, indicating that it represents the CME of this region. After removing CME, the canonical correlation coefficients and root mean square error of GNSS residual time series, as well as the amplitudes of power-law noises (PLN), are obviously decreased in all three directions. However, the white noise (WN) amplitudes are found to diminish exclusively in the North and East component, not in the Up components. Moreover, the average velocity differences before and after filtering CME are 0.19 mm/year, 0.03 mm/year, and −0.56 mm/year for the NEU components, respectively, indicating that CME has an influence on the GNSS station velocity estimation. The velocity uncertainty is also reduced by 43.51%, 38.64%, and 40.39% on average for the NEU components, respectively, implying that the velocity estimates are more reliable and accurate after removing CME. Therefore, we conclude that the new WEMPCA approach provides an efficient solution to detect and mitigate CME from the noisy and missing GNSS position time series.
Vegetation Browning Trends in Spring and Autumn over Xinjiang, China, during the Warming Hiatus
Authors: Li Moyan , Yao Junqiang , Guan Jingyun , Zheng Jianghua
Published: March 2022

Satellite-derived vegetation records (GIMMS3g-NDVI) report that climate warming promotes vegetation greening trends; however, the climate impacts on vegetation growth during the global warming hiatus period (1998–2012) remain unclear. In this study, we focused on the vegetation change trend in Xinjiang in spring and autumn before and during the recent warming hiatus period, and their climate-driving mechanisms, which have not been examined in previous studies. Based on satellite records, our results indicated that the summer normalized difference vegetation index (NDVI) in Xinjiang experienced a greening trend, while a browning trend existed in spring and autumn during this period. The autumn NDVI browning trend in Xinjiang was larger than that in spring; however, the spring NDVI displayed a higher correlation with climatic factors than did the autumn NDVI. During the warming hiatus, spring climatic factors were the main controlling factors of spring NDVI, and spring vapor pressure deficit (VPD) had the highest positive correlation with spring NDVI, followed by spring temperature. The larger increase in air temperature in spring than in autumn resulted in increased VPD differences in spring and autumn. In autumn, summer climatic factors (e.g., VPD, WS, RH, and precipitation) were significantly correlated with the autumn NDVI during the warming hiatus. However, the autumn temperature was weakly correlated with the autumn NDVI. Our results have significant implications for understanding the response of vegetation growth to recent and future climatic conditions.
An Interpretation Approach of Ascending–Descending SAR Data for Landslide Identification
Authors: Ren Tianhe , Gong Wenping , Gao Liang , Zhao Fumeng , Cheng Zhan
Published: March 2022

The technique of interferometric synthetic aperture radar (InSAR) is increasingly employed for landslide detection over large areas, even though the limitations of initial InSAR analysis results have been well acknowledged. Steep terrain in mountainous areas may cause geometric distortions of SAR images, which could affect the accuracy of InSAR analysis results. In addition, due to the existence of massive ground deformation points in the initial InSAR analysis results, accurate landslide recognition from the initial results is challenging. To efficiently identify potential landslide areas from the ascending–descending SAR datasets, this paper presents a novel interpretation approach to analyze the initial time-series InSAR analysis results. Within the context of the proposed approach, SAR visibility analysis, conversion analysis of deformation rates obtained from the time-series InSAR analysis, and spatial analysis and statistics tools for cluster extraction are incorporated. The effectiveness of the proposed approach is illustrated through a case study of landslide identification in Danba, a county in Sichuan, China. The potential landslide regions in the study area are identified based on the interpretation of small baseline subset InSAR (SBAS-InSAR) results, obtained with ascending–descending Sentinel-1A datasets. Finally, on the basis of the field survey results, a total of 21 landslides are detected in the potential landslide regions identified, through which the results obtained from the proposed interpretation approach are tested.