bluuebunny/crossref_metadata_2025 huggingface dataset:
Demonstration of the Temporal Evolution of Tropical Cyclone “Phailin” Using Gray-Zone Simulations and Decadal Variability of Cyclones over the Bay of Bengal in a Warming Climate
Authors: Pradhan Prabodha Kumar , Kumar Vinay , Khadgarai Sunilkumar , Rao S. Vijaya Bhaskara , Sinha Tushar , Kattamanchi Vijaya Kumari , Pattnaik Sandeep
Published: September 2021

The intensity and frequency variability of cyclones in the North Indian Ocean (NIO) have been amplified over the last few decades. The number of very severe cyclonic storms (VSCSs) over the North Indian Ocean has increased over recent decades. “Phailin”, an extreme severe cyclonic storm (ESCS), occurred during 8–13 October 2013 over the Bay of Bengal and made landfall near the Gopalpur coast of Odisha at 12 UTC on 12 October. It caused severe damage here, as well as in the coastal Odisha, Andhra Pradesh, and adjoining regions due to strong wind gusts (~115 knot/h), heavy precipitation, and devastating storm surges. The fidelity of the WRF model in simulating the track and intensity of tropical cyclones depends on different cloud microphysical parameterization schemes. Thus, four sensitivity simulations were conducted for Phailin using double-moment and single-moment microphysical (MP) parameterization schemes. The experiments were conducted to quantify and characterize the performance of such MP schemes for Phailin. The simulations were performed by the advanced weather research and forecasting (WRF-ARW) model. The model has two interactive domains covering the entire Bay of Bengal and adjoining coastal Odisha on 25 km and 8.333 km resolutions. Milbrandt–Yau (MY) double-moment and WRF single-moment microphysical schemes, with 6, 5, and 3 classes of hydrometeors, i.e., WSM6, WSM5, and WSM3, were used for the simulation. Experiments for Phailin were conducted for 126 h, starting from 00 UTC 08 October to 06 UTC 13 October 2013. It was found that the track, intensity, and structure of Phailin are highly sensitive to the different microphysical parameterization schemes. Further, the precipitation and cloud distribution were studied during the ESCS stage of Phailin. The microphysics schemes (MY, WSM3, WSM5, WSM6), along with Grell–Devenyi ensemble convection scheme predicted landfall of Phailin over the Odisha coast with significant track errors. Supply of moisture remains a more crucial component than SST and wind shear for rapid intensification of the Phailin 12 h before landfall over the Bay of Bengal. Finally, the comparison of cyclone formation between two decades 2001–2010 and 2011–2020 over the Bay of Bengal inferred that the increased numbers of VSCS are attributed to the supply of abundant moisture at low levels in the recent decade 2011–2020.
Cryptic Diversity in Cladosporium cladosporioides Resulting from Sequence-Based Species Delimitation Analyses
Authors: Becchimanzi Andrea , Zimowska Beata , Nicoletti Rosario
Published: September 2021

Cladosporium cladosporioides is an extremely widespread fungus involved in associations ranging from mutualistic to pathogenic and is the most frequently represented Cladosporium species in sequence databases, such as Genbank. The taxonomy of Cladosporium species, currently based on the integration of molecular data with morphological and cultural characters, is in frequent need of revision. Hence, the recently developed species delimitation methods can be helpful to explore cryptic diversity in this genus. Considering a previous study that reported several hypothetical species within C. cladosporioides, we tested four methods of species delimitation using the combined DNA barcodes internal transcribed spacers, translation elongation factor 1-α and actin 1. The analyses involved 105 isolates, revealing that currently available sequences of C. cladosporioides in GenBank actually represent more than one species. Moreover, we found that eight isolates from this set should be ascribed to Cladosporium anthropophilum. Our results revealed a certain degree of discordance among species delimitation methods, which can be efficiently treated using conservative approaches in order to minimize the risk of considering false positives.
Gemcitabine Direct Electrochemical Detection from Pharmaceutical Formulations Using a Boron-Doped Diamond Electrode
Authors: Rus Iulia , Pusta Alexandra , Tertiș Mihaela , Barbălată Cristina , Tomuță Ioan , Săndulescu Robert , Cristea Cecilia
Published: September 2021

The development of fast and easy-to-use methods for gemcitabine detection is of great interest for pharmaceutical formulation control in both research laboratories and hospitals. In this study, we report a simple, fast and direct electrochemical method for gemcitabine detection using a boron-doped diamond electrode. The electrochemical oxidation of gemcitabine on a boron-doped diamond electrode was found to be irreversible in differential pulse voltammetry, and scan rate influence studies demonstrated that the process is diffusion-controlled. The influence of the pH and supporting electrolytes were also tested, and the optimized differential pulse voltammetry method was linear in the range of 2.5–50 μg/mL, with a detection limit of 0.85 μg/mL in phosphate-buffered saline (pH 7.4; 0.1 M). An amperometric method was also optimized for gemcitabine detection. The linear range of the method was 0.5–65 μg/mL in phosphate-buffered saline of pH 7.4 as well as pH 5.5, the limit of detection being 0.15 μg/mL. The optimized differential pulse voltammetry and amperometric detection strategies were successfully applied to pharmaceutical formulations, and the results were compared to those obtained by high-performance liquid chromatography and UV-Vis spectrophotometry with good correlations.
Gene Dosage Analysis on the Single-Cell Transcriptomes Linking Cotranslational Protein Targeting to Metastatic Triple-Negative Breast Cancer
Authors: Liu Yining , Zhao Min
Published: September 2021

Many recent efforts have been put into the association between expression heterogeneity and different cell types and states using single-cell RNA transcriptome analysis. There is only limited understanding of gene dosage effects for the genetic heterogeneity at the single-cell level. By focusing on concordant copy number variation (CNV) and expression, we presented a computational framework to explore dosage effect for aggressive metastatic triple-negative breast cancer (TNBC) at the single-cell level. In practice, we collected CNV and single-cell expression data from the same patients with independent technologies. By focusing on 47,198 consistent copy number gains (CNG) and gene up-regulation from 1145 single cells, ribosome proteins with important roles in protein targeting were enriched. Independent validation in another metastatic TNBC dataset further prioritized signal recognition particle-dependent protein targeting as the top functional module. More interesting, the increased ribosome gene copies in TNBC may associate with their enhanced stemness and metastatic potential. Indeed, the prioritization of a well-upregulated functional module confirmed by high copy numbers at the single-cell level and contributing to patient survival may indicate the possibility of targeted therapy based on ribosome proteins for TNBC.
The Metabolic Response of Various Cell Lines to Microtubule-Driven Uptake of Lipid- and Polymer-Coated Layer-by-Layer Microcarriers
Authors: Claus Claudia , Fritz Robert , Schilling Erik , Reibetanz Uta
Published: September 2021

Lipid structures, such as liposomes or micelles, are of high interest as an approach to support the transport and delivery of active agents as a drug delivery system. However, there are many open questions regarding their uptake and impact on cellular metabolism. In this study, lipid structures were assembled as a supported lipid bilayer on top of biopolymer-coated microcarriers based on the Layer-by-Layer assembly strategy. The functionalized microcarriers were then applied to various human and animal cell lines in addition to primary human macrophages (MΦ). Here, their influence on cellular metabolism and their intracellular localization were detected by extracellular flux analysis and immunofluorescence analysis, respectively. The impact of microcarriers on metabolic parameters was in most cell types rather low. However, lipid bilayer-supported microcarriers induced a decrease in oxygen consumption rate (OCR, indicative for mitochondrial respiration) and extracellular acidification rate (ECAR, indicative for glycolysis) in Vero cells. Additionally, in Vero cells lipid bilayer microcarriers showed a more pronounced association with microtubule filaments than polymer-coated microcarrier. Furthermore, they localized to a perinuclear region and induced nuclei with some deformations at a higher rate than unfunctionalized carriers. This association was reduced through the application of the microtubule polymerization inhibitor nocodazole. Thus, the effect of respective lipid structures as a drug delivery system on cells has to be considered in the context of the respective target cell, but in general can be regarded as rather low.
Current Trends on Protein Driven Bioinks for 3D Printing
Authors: Veiga Anabela , Silva Inês V. , Duarte Marta M. , Oliveira Ana L.
Published: September 2021

In the last decade, three-dimensional (3D) extrusion bioprinting has been on the top trend for innovative technologies in the field of biomedical engineering. In particular, protein-based bioinks such as collagen, gelatin, silk fibroin, elastic, fibrin and protein complexes based on decellularized extracellular matrix (dECM) are receiving increasing attention. This current interest is the result of protein’s tunable properties, biocompatibility, environmentally friendly nature and possibility to provide cells with the adequate cues, mimicking the extracellular matrix’s function. In this review we describe the most relevant stages of the development of a protein-driven bioink. The most popular formulations, molecular weights and extraction methods are covered. The different crosslinking methods used in protein bioinks, the formulation with other polymeric systems or molecules of interest as well as the bioprinting settings are herein highlighted. The cell embedding procedures, the in vitro, in vivo, in situ studies and final applications are also discussed. Finally, we approach the development and optimization of bioinks from a sequential perspective, discussing the relevance of each parameter during the pre-processing, processing, and post-processing stages of technological development. Through this approach the present review expects to provide, in a sequential manner, helpful methodological guidelines for the development of novel bioinks.
Development of Antimicrobial Nitric Oxide-Releasing Fibers
Authors: Wang Daniel C. , Clark Justin R. , Lee Richard , Nelson Adam H. , Maresso Anthony W. , Acharya Ghanashyam , Shin Crystal S.
Published: September 2021

Nitric oxide (NO) is a highly reactive gas molecule, exhibiting antimicrobial properties. Because of its reactive nature, it is challenging to store and deliver NO efficiently as a therapeutic agent. The objective of this study was to develop NO-releasing polymeric fibers (NO-fibers), as an effective delivery platform for NO. NO-fibers were fabricated with biopolymer solutions of polyvinyl pyrrolidone (PVP) and ethylcellulose (EC), and derivatives of N-diazeniumdiolate (NONOate) as NO donor molecules, using an electrospinning system. We evaluated in vitro NO release kinetics, along with antimicrobial effects and cytotoxicity in microorganisms and human cell culture models. We also studied the long-term stability of NONOates in NO-fibers over 12 months. We demonstrated that the NO-fibers could release NO over 24 h, and showed inhibition of the growth of Pseudomonas aeruginosa (P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA), without causing cytotoxicity in human cells. NO-fibers were able to store NONOates for over 12 months at room temperature. This study presents the development of NO-fibers, and the feasibility of NO-fibers to efficiently store and deliver NO, which can be further developed as a bandage.
Physicochemical Stability and Compatibility Testing of Voriconazole in All-in-One Parenteral Nutrition Admixtures
Authors: Reber Emilie , Neyer Peter , Schönenberger Katja A. , Saxer Christoph , Bernasconi Luca , Stanga Zeno , Huber Andreas , Hammerer-Lercher Angelika , Mühlebach Stefan
Published: September 2021

(1) Drug compatibility with all-in-one (AiO) parenteral nutrition (PN) admixtures is a very important pharmaceutical quality issue to be answered based on appropriate laboratory testing. We assessed voriconazole (V), a poorly water-soluble (logP ≈ 1) single-daily dosed antifungal drug monitored in patients and thus candidate for AiO PN admixing for convenient and safe patient care. We evaluated V compatibility and stability in AiO PN admixtures through adapted therapeutic drug monitoring method (drug stability) and visual microscopic emulsion stability by lipid droplets analysis improved by an automated microscopic digital assessment. (2) V was added in concentrations of 0.05/0.25/0.5 mg/mL (143.1/715.7/1431.5 µM), correlating to daily therapeutic dosing, to three commercially available industrial AiO PN admixtures. Three aliquots were stored in the refrigerator (4 °C), at room temperature (24 °C) and under stress conditions in a water bath (37 °C). Samples taken at 0/24/48/72/168 h after admixing were subjected to a stability-indicating one-week analysis. Assessment included visual examination, lipid droplet measurement according to an established and validated method (bright-field microscopy using oil immersion), pH measurement (glass electrode) and V identification/quantification (LC–MS/MS). (3) After one week, all samples at 37 °C showed slight yellow discoloration. The pH values remained stable. All samples met specifications for lipid droplets according to size (upper size ≤8 µm, mean size <4.5 ± 2 µm) and number (n ≤ 9 lipid droplets >5 µm). V concentrations were within an acceptable range, calculated for every timepoint as percent of the theoretical concentration spiked into the AiO PN. The median recovery was 98.2% (min–max, 90–112%). (4) At therapeutic doses, commercial V formulations were compatible and stable within specifications over one week in commonly used volumes of commercial AiO PN admixtures at 4–37 °C.
Synergy of Hydeal-D® and Hyaluronic Acid for Protecting and Restoring Urothelium: In Vitro Characterization
Authors: Ruggeri Marco , Pavan Mauro , Soato Matteo , Panfilo Susi , Barbera Carlo , Galesso Devis , Miele Dalila , Rossi Silvia , Di Lucia Alba , Ferrari Franca , Sandri Giuseppina
Published: September 2021

Interstitial cystitis (IC) or painful bladder syndrome is a chronic dysfunction due to an inflammatory condition, characterized by bladder pain and urinary frequency. Currently, no gold standard therapy is available since IC does not respond to conventional ones. Given these premises, the aim of this work was the in vitro characterization of biological properties (mucoadhesion and anti-inflammatory activity) of a commercial product (HydealCyst–HydC) based on hyaluronic acid (HA) and the benzyl ester of HA (Hydeal-D®) intended for bladder instillation to restore and/or protect the urothelial layer of glycosamino glycans (GAGs). The in vitro characterization demonstrated that an interaction product is formed between HA and Hydeal-D® that has a role in the rheological behavior and mucoadhesive properties. HA was identified as a key component to form the mucoadhesive joint, while the interaction of HA with Hydeal-D® improved polysaccharide stability and prolonged the activity ex vivo. Moreover, HydC is cytocompatible with urothelial cells (HTB-4) and possesses an anti-inflammatory effect towards these cells by decreasing the secretion of IL-6 and IL-8, which were both increased in patients with IC, and by increasing the secretion of sulfated GAGs. These two findings, along with the resilience properties of the formulation due to mucoadhesion, suggest the active role of HydC in protecting and restoring urothelium homeostasis.
Impact of Alkanediols on Stratum Corneum Lipids and Triamcinolone Acetonide Skin Penetration
Authors: Sigg Melanie , Daniels Rolf
Published: September 2021

Alkanediols are widely used as multifunctional ingredients in dermal formulations. In addition to their preservative effect, considering their possible impact on drug penetration is also essential for their use. In the present study, the influence of 2-methyl-2,4-pentanediol, 1,2-pentanediol, 1,2-hexanediol and 1,2-octanediol on the skin penetration of triamcinolone acetonide from four different semisolid formulations was investigated. Furthermore, confocal Raman spectroscopy measurements were performed to examine the influence of the alkanediols on stratum corneum lipid content and order. Alkanediols were found to increase the penetration of triamcinolone acetonide. However, the extent depends strongly on the formulation used. In certain formulations, 1,2-pentanediol showed the highest effect, while in others the penetration-enhancing effect increased with the alkyl chain length of the alkanediol used. None of the tested alkanediols extracted lipids from the stratum corneum nor reduced its thickness. Notwithstanding the above, the longer-chained alkanediols cause the lipids to be converted to a more disordered state, which favors drug penetration. This behavior could not be detected for the shorter-chained alkanediols. Therefore, their penetration-enhancing effect is supposed to be related to an interaction with the hydrophilic regions of the stratum corneum.
Recent Advances in ROS-Sensitive Nano-Formulations for Atherosclerosis Applications
Authors: Ji Hao , Peng Renyi , Jin Libo , Ma Jiahui , Yang Qinsi , Sun Da , Wu Wei
Published: September 2021

Over the past decade, ROS-sensitive formulations have been widely used in atherosclerosis applications such as ROS scavenging, drug delivery, gene delivery, and imaging. The intensified interest in ROS-sensitive formulations is attributed to their unique self-adaptive properties, involving the main molecular mechanisms of solubility switch and degradation under the pathological ROS differences in atherosclerosis. This review outlines the advances in the use of ROS-sensitive formulations in atherosclerosis applications during the past decade, especially highlighting the general design requirements in relation to biomedical functional performance.
Predicting Drug Release from 3D Printed Oral Medicines Based on the Surface Area to Volume Ratio of Tablet Geometry
Authors: Windolf Hellen , Chamberlain Rebecca , Quodbach Julian
Published: September 2021

3D printing offers the advantage of being able to modify dosage form geometry, which can be exploited to modify release characteristics. In this study, we investigated the influence of the surface area to volume ratio (SA/V) to change and predict release profiles of 3D printed dosage forms. Geometries with varying SA/V and dosages were designed and printed, and drug dissolution was investigated. Three drug substances were used: pramipexole, levodopa (both BCS I) and praziquantel (BCS II). Two polymers were chosen as matrix formers: polyvinyl alcohol (water-soluble) and ethylene vinyl acetate (inert). Drug release was characterized using the mean dissolution time (MDT) and established equations that describe complete dissolution curves were applied. Predictions were validated with previously un-printed dosage forms. Based on an identified MDT-SA/V correlation, the MDT can be predicted with a deviation of ≤5 min for a given SA/V. Using correlations of fit parameters and SA/V, RMSEP values of 0.6–2.8% and 1.6–3.4% were obtained for the BCS I formulations and RMSEP values of 1.0–3.8% were obtained for the BCS II formulation, indicating accurate prediction over a wide range of dissolution profiles. With this approach, MDT and release profiles of dosage forms with a given SA/V can be precisely predicted without performing dissolution tests and vice versa, the required SA/V can be predicted for a desired release profile.
Nanostructured Lipid Carriers for the Formulation of Topical Anti-Inflammatory Nanomedicines Based on Natural Substances
Authors: Ferreira Kézia Cristine Barbosa , Valle Ana Beatriz Caribé dos Santos , Paes Camila Quinetti , Tavares Guilherme Diniz , Pittella Frederico
Published: September 2021

The main function of the skin is to protect the body from the external environment. However, the skin can undergo inflammatory processes, due to genetic, hormonal, or environmental factors. When the defense system is overloaded, there is an increase in pro-inflammatory mediators and reactive oxygen species (ROS), which results in skin disorders. Among the substances used to treat these inflammatory processes, many natural substances with anti-inflammatory and antioxidant properties are being studied: nature is yet an abundant source to obtain diverse pharmacological actives. The treatment of skin diseases is usually focused on topical application, as it reduces the risk of systemic side effects and prevents drug degradation by first-pass metabolism. Thus, the properties of drug delivery vehicles can facilitate or inhibit its permeation. Due to the hydrophobic nature of the skin, a promising strategy to improve dermal drug penetration is the use of lipid-based nanoparticles, such as nanostructured lipid carriers (NLC). Therefore, in this review, we present NLC as a tool to improve dermal administration of natural substances with anti-inflammatory properties.
Plant Growth Regulators Application Enhance Tolerance to Salinity and Benefit the Halophyte Plantago coronopus in Saline Agriculture
Authors: Bueno Milagros , Cordovilla María del Pilar
Published: September 2021

Climate change, soil salinisation and desertification, intensive agriculture and the poor quality of irrigation water all create serious problems for the agriculture that supplies the world with food. Halophyte cultivation could constitute an alternative to glycophytic cultures and help resolve these issues. Plantago coronopus can be used in biosaline agriculture as it tolerates salt concentrations of 100 mM NaCl. To increase the salt tolerance of this plant, plant growth regulators such as polyamine spermidine, salicylic acid, gibberellins, cytokinins, and auxins were added in a hydroponic culture before the irrigation of NaCl (200 mM). In 45-day-old plants, dry weight, water content, osmolyte (sorbitol), antioxidants (phenols, flavonoids), polyamines (putrescine, spermidine, spermine (free, bound, and conjugated forms)) and ethylene were determined. In non-saline conditions, all plant regulators improved growth while in plants treated with salt, spermidine application was the most effective in improving growth, osmolyte accumulation (43%) and an increase of antioxidants (24%) in P. coronopus. The pretreatments that increase the sorbitol content, endogenous amines (bound spermine fraction), phenols and flavonoids may be the most effective in protecting to P. coronopus against stress and, therefore, could contribute to improving the tolerance to salinity and increase nutritional quality of P. coronopus.
Drought Stress Memory at the Plant Cycle Level: A Review
Authors: Jacques Cécile , Salon Christophe , Barnard Romain L. , Vernoud Vanessa , Prudent Marion
Published: September 2021

Plants are sessile organisms whose survival depends on their strategy to cope with dynamic, stressful conditions. It is urgent to improve the ability of crops to adapt to recurrent stresses in order to alleviate the negative impacts on their productivity. Although our knowledge of plant adaptation to drought has been extensively enhanced during the last decades, recent studies have tackled plant responses to recurrent stresses. The present review synthesizes the major findings from studies addressing plant responses to multiple drought events, and demonstrates the ability of plants to memorize drought stress. Stress memory is described as a priming effect allowing a different response to a reiterated stress when compared to a single stress event. Here, by specifically focusing on water stress memory at the plant cycle level, we describe the different underlying processes at the molecular, physiological and morphological levels in crops as well as in the model species Arabidopsis thaliana. Moreover, a conceptual analysis framework is proposed to study drought stress memory. Finally, the essential role of interactions between plants and soil microorganisms is emphasized during reiterated stresses because their plasticity can play a key role in supporting overall plant resilience.
Low Temperature Stress Mediates the Antioxidants Pool and Chlorophyll Fluorescence in Vitis vinifera L. Cultivars
Authors: Aazami Mohammad A. , Asghari-Aruq Majid , Hassanpouraghdam Mohammad B. , Ercisli Sezai , Baron Mojmir , Sochor Jiri
Published: September 2021

Grapes are sensitive to early autumn and spring low temperature damage. The current study aimed to assay the effects of cold stress (+1 °C for 4, 8, and 16 h) on three grapevine cultivars (Ghiziluzum, Khalili, and Perllete). The results showed that cold stress caused significant changes in the antioxidant and biochemicals content in the studied cultivars. Furthermore, examining the chlorophyll fluorescence indices, cold stress caused a significant increase in minimal fluorescence (F0), a decrease in maximal fluorescence (Fm), and the maximum photochemical quantum yield of photosystem II (Fv/Fm) in all cultivars. Among the studied cultivars, ‘Perllete’ had the highest increase in proline content and activity of antioxidant enzymes and also had the lowest accumulation of malondialdehyde, hydrogen peroxide, electrolyte leakage, and F0, as well as less of a decrease in Fm and Fv/Fm, and had a higher tolerance to cold stress than ‘Ghiziluzum’ and ‘Khalili’. ‘Perllete’ and ‘Ghiziluzum’ showed reasonable tolerance to the low temperature stress. ‘Khalili’ was sensitive to the stress. The rapid screening of grapevine cultivars in early spring low temperatures is applicable with the assaying of some biomolecules and chlorophyll fluorescence.
Rapid On-Farm Testing of Resistance in Lolium rigidum to Key Pre- and Post-Emergence Herbicides
Authors: Perez Martina Badano , Beckie Hugh J. , Cawthray Gregory R. , Goggin Danica E. , Busi Roberto
Published: September 2021

Overreliance on herbicides for weed control is conducive to the evolution of herbicide resistance. Lolium rigidum (annual ryegrass) is a species that is prone to evolve resistance to a wide range of herbicide modes of action. Rapid detection of herbicide-resistant weed populations in the field can aid farmers to optimize the use of effective herbicides for their control. The feasibility and utility of a rapid 7-d agar-based assay to reliably detect L. rigidum resistant to key pre- and post-emergence herbicides including clethodim, glyphosate, pyroxasulfone and trifluralin were investigated in three phases: correlation with traditional pot-based dose-response assays, effect of seed dormancy, and stability of herbicides in agar. Easy-to-interpret results were obtained using non-dormant seeds from susceptible and resistant populations, and resistance was detected similarly as pot-based assays. However, the test is not suitable for trifluralin because of instability in agar as measured over a 10-d period, as well as freshly-harvested seeds due to primary dormancy. This study demonstrates the utility of a portable and rapid assay that allows for on-farm testing of clethodim, glyphosate, and pyroxasulfone resistance in L. rigidum, thereby aiding the identification and implementation of effective herbicide control options.
Effect of the Molecular Structure of TPU on the Cellular Structure of Nanocellular Polymers Based on PMMA/TPU Blends
Authors: Sánchez-Calderón Ismael , Bernardo Victoria , Santiago-Calvo Mercedes , Naji Haneen , Saiani Alberto , Rodríguez-Pérez Miguel
Published: September 2021

In this work, the effects of thermoplastic polyurethane (TPU) chemistry and concentration on the cellular structure of nanocellular polymers based on poly(methyl-methacrylate) (PMMA) are presented. Three grades of TPU with different fractions of hard segments (HS) (60%, 70%, and 80%) have been synthesized by the prepolymer method. Nanocellular polymers based on PMMA have been produced by gas dissolution foaming using TPU as a nucleating agent in different contents (0.5 wt%, 2 wt%, and 5 wt%). TPU characterization shows that as the content of HS increases, the density, hardness, and molecular weight of the TPU are higher. PMMA/TPU cellular materials show a gradient cell size distribution from the edge of the sample towards the nanocellular core. In the core region, the addition of TPU has a strong nucleating effect in PMMA. Core structure depends on the HS content and the TPU content. As the HS or TPU content increases, the cell nucleation density increases, and the cell size is reduced. Then, the use of TPUs with different characteristics allows controlling the cellular structure. Nanocellular polymers have been obtained with a core relative density between 0.15 and 0.20 and cell sizes between 220 and 640 nm.
Shape Memory Composite Sandwich Structures with Self-Healing Properties
Authors: Quadrini Fabrizio , Bellisario Denise , Iorio Leandro , Santo Loredana , Pappas Panagiotis , Koutroumanis Nikolaos , Anagnostopoulos George , Galiotis Costas
Published: September 2021

In this study, Polyurea/Formaldehyde (PUF) microcapsules containing Dicyclopentadiene (DCPD) as a healing substance were fabricated in situ and mixed at relatively low concentrations (<2 wt%) with a thermosetting polyurethane (PU) foam used in turn as the core of a sandwich structure. The shape memory (SM) effect depended on the combination of the behavior of the PU foam core and the shape memory polymer composite (SMPC) laminate skins. SMPC laminates were manufactured by moulding commercial carbon fiber-reinforced (CFR) prepregs with a SM polymer interlayer. At first, PU foam samples, with and without microcapsules, were mechanically tested. After, PU foam was inserted into the SMPC sandwich structure. Damage tests were carried out by compression and bending to deform and break the PU foam cells, and then assess the structure self-healing (SH) and recovery capabilities. Both SM and SH responses were rapid and thermally activated (120 °C). The CFR-SMPC skins and the PU foam core enable the sandwich to exhibit excellent SM properties with a shape recovery ratio up to 99% (initial configuration recovery). Moreover, the integration of microcapsules (0.5 wt%) enables SH functionality with a structural restoration up to 98%. This simple process makes this sandwich structure ideal for different industrial applications.
Solute Diffusion into Polymer Swollen by Supercritical CO2 by High-Pressure Electron Paramagnetic Resonance Spectroscopy and Chromatography
Authors: Gromov Oleg I. , Kostenko Mikhail O. , Petrunin Alexander V. , Popova Anastasia A. , Parenago Olga O. , Minaev Nikita V. , Golubeva Elena N. , Melnikov Mikhail Ya.
Published: September 2021

High-pressure electron paramagnetic resonance (EPR) was used to measure translational diffusion coefficients (Dtr) of a TEMPONE spin probe in poly(D,L-lactide) (PDLLA) and swollen in supercritical CO2. Dtr was measured on two scales: macroscopic scale (>1 μm), by measuring spin probe uptake by the sample; and microscopic scale (<10 nm), by using concentration-dependent spectrum broadening. Both methods yield similar translational diffusion coefficients (in the range 5–10 × 10−12 m2/s at 40–60 °C and 8–10 MPa). Swollen PDLLA was found to be homogeneous on the nanometer scale, although the TEMPONE spin probe in the polymer exhibited higher rotational mobility (τcorr = 6 × 10−11 s) than expected, based on its Dtr. To measure distribution coefficients of the solute between the swollen polymer and the supercritical medium, supercritical chromatography with sampling directly from the high-pressure vessel was used. A distinct difference between powder and bulk polymer samples was only observed at the start of the impregnation process.
Manipulating Crystallization for Simultaneous Improvement of Impact Strength and Heat Resistance of Plasticized Poly(l-lactic acid) and Poly(butylene succinate) Blends
Authors: Kajornprai Todsapol , Suttiruengwong Supakij , Sirisinha Kalyanee
Published: September 2021

Crystalline morphology and phase structure play a decisive role in determining the properties of polymer blends. In this research, biodegradable blends of poly(l-lactic acid) (PLLA) and poly(butylene succinate) (PBS) have been prepared by melt-extrusion and molded into specimens with rapid cooling. The crystalline morphology (e.g., crystallinity, crystal type and perfection) is manipulated by annealing the molded products from solid-state within a short time. This work emphasizes on the effects of annealing conditions on crystallization and properties of the blends, especially impact toughness and thermal stability. Phase-separation morphology with PBS dispersed particles smaller than 1 μm is created in the blends. The blend properties are successfully dictated by controlling the crystalline morphology. Increasing crystallinity alone does not ensure the enhancement of impact toughness. A great improvement of impact strength and heat resistance is achieved when the PLLA/PBS (80/20) blends are plasticized with 5% medium molecular-weight poly(ethylene glycol), and simultaneously heat-treated at a temperature close to the cold-crystallization of PLLA. The plasticized blend annealed at 92 °C for only 10 min exhibits ten-fold impact strength over the starting PLLA and slightly higher heat distortion temperature. The microscopic study demonstrates the fracture mechanism changes from crazing to shear yielding in this annealed sample.
Polymer for Internal Hydrophobization of Cement-Based Materials: Design, Synthesis, and Properties
Authors: Liu Xiao , Song Xiaofei , Wang Ziming , Xia Chunlei , Li Ting , Li Xiaoning , Xu Qian , Cui Suping , Qian Shanshan
Published: September 2021

A series of novel comb-like poly(butyl acrylate)-g-poly(dimethylaminoethyl methacrylate) (PBA-g-PDMAEMA) with different side chain lengths were designed and successfully synthesized by the “first main chain then side chain” method. Infrared Spectroscopy (IR), 1H Nuclear Magnetic Resonance (1H NMR), and gel permeation chromatography (GPC) were used for structural confirmation and molecular weight characterization. This polymer exhibited responsive behavior from hydrophilicity to hydrophobicity under the alkaline environment of cement-based materials, with the contact angle of 105.6°, a decreased evaporation rate, and a hydrophile–lipophile balance (HLB) value. A significant internal hydrophobic effect on cement mortar was shown in the water absorption rate, which decreased by 75.2%, and a dry shrinkage-reducing rate of more than 30%. Furthermore, this polymer can effectively slow the exothermic rate, reduce the heat release, and delay the exothermic peak of cement hydration. It was interesting that these properties showed a direct correlation with the side chain length of the comb polymer. The aims of this study are to provide a new avenue to synthesize polymers with the spontaneous hydrophilicity–hydrophobicity transition in the cement system, achieving excellent internal hydrophobicity of cement-based materials, and to offer a promising alternative to resist external erosion for improving the durability and service life of cement-based materials.
Application of Aramid Nanofibers in Nanocomposites: A Brief Review
Authors: Fan Yangyang , Li Zhihua , Wei Junchao
Published: September 2021

The diameter of fibers is a critical factor in determining their final applications. When the diameter of aramid fibers changes from microns to nanoscale, its range of applications will be greatly extended. In this short review, the preparation of aramid nanofibers (ANFs) with diameters from ten nanometers to more than one hundred nanometers is introduced. Due to their excellent mechanical properties and their chemical and thermal stability, ANFs have been widely used as novel nanomaterials and composited with other materials, mainly for use in reinforced composites, energy storage, filtration and adsorption, biomedicine and electromagnetic fields. In this short review, the application of ANFs and their composites during the last 10 years is concisely summarized and a brief perspective on ANFs and their composites is also presented.
Self-Heating Mould for Composite Manufacturing
Authors: Kondratiev Andrii , Píštěk Václav , Purhina Svitlana , Shevtsova Maryna , Fomina Anna , Kučera Pavel
Published: September 2021

The shipbuilding industry, engine manufacturing, aviation, rocket and space technology are promising fields of application for polymeric composite materials. Shape-generating moulding tools with internal heating are used for the creation of a more economically viable method of moulding of internally heated composite structures. The use of a fine-fibered resistive structure in the heated tools allows implementation of effective heating of the composite and elimination of the need for expensive and energy-intensive heating equipment. The aim of this paper was the reduction of energy consumption for internally heated moulding tools by choosing the optimal parameters for their resistive layer. A method for determination of the parameters of the moulding tool resistive layer was developed. This method allows calculation of the heating layer parameters and implementation of the specified time–temperature regime for moulding of the composite structure. It was shown that energy saving for the heated fiberglass shape-generating moulding tools was from 40 to 60%. It was found that the increase in the thickness of the moulded package of the polymeric composite material resulted not only in a higher supplied power for the heating system, but also in a complication of the method for system control, because of the growing exothermic effect of the binder curing reaction. For composite products based on Hysol EA 9396 binder, thicknesses more than 4 mm are critical, because it is not possible to cope with the self-heating effect only by cooling with ambient air already utilized at the twentieth minute of the moulding process. The influence of the physical and mechanical characteristics of the moulding tool material and stiffening ribs was analysed in terms of energy consumption and controllability of the heating system. Fiberglass shows the lowest energy consumption. Heating of the aluminium and steel moulding tools for the same purpose will require 20% and 45% more power, respectively. An increase in the number of stiffening ribs has a strong effect on the heat removal of the heating system. With a small number of aluminium ribs it is not possible to maintain the specified temperature–time regime for a fiberglass moulded package of 5 mm thick with the use of the equipment. However, when the number of stiffeners is increased to 10, the exothermic effect of the reaction becomes smoother and then the heating equipment can cope with the task. An experimental prototype of heating equipment of moulding tools for the manufacturing of structures of polymeric composite materials, as well as a flexible thermal blanket for repair of non-separable structures, were developed. The results can be the basis for a new method of optimal design of parameters of moulding tool structure at minimal heat removal to the environment.
Optimisation of Through-Thickness Embedding Location of Fibre Bragg Grating Sensor in CFRP for Impact Damage Detection
Authors: Rocha Helena , Lafont Ugo , Nunes João P.
Published: September 2021

Aerospace composites are susceptible to barely visible impact damage (BVID) produced by low-velocity-impact (LVI) events. Fibre Bragg grating (FBG) sensors can detect BVID, but often FBG sensors are embedded in the mid-plan, where residual strains produced by impact damage are lower, leading to an undervaluation of the damage severity. This study compares the residual strains produced by LVI events measured by FBG embedded at the mid-plan and other through-thickness locations of carbon fibre reinforced polymer (CFRP) composites. The instrumented laminates were subjected to multiple low-velocity impacts while the FBG signals were acquired. The FBG sensor measurements allowed not only for the residual strain after damage to be measured, but also for a strain peak at the time of impact to be detected, which is an important feature to identify the nature and presence of BVID in real-life applications. The results allowed an adequate optical fibre (OF) embedding location to be selected for BVID detection. The effect of small- and large-diameter OF on the impact resistance of the CFRP was compared.
Japan’s Sacred Sumo and the Exclusion of Women: The Olympic Male Sumo Wrestler (Part 1)
Authors: DeWitt Lindsey E.
Published: September 2021

The 2020 Summer Olympic Games in Tokyo offer a fitting and timely point of departure to consider the religion-based exclusion of women and, by extension, to peer into the nation-culture-religion-gender nexus in Japan. The Japan Sumo Association, a quasi-governmental corporation, champions itself as the custodian of a divine affair cultivated by male deities and mortal men, and exclusive of women. The Sumo Association bans women and girls from entering or even touching the wrestling ring, lest they violate sumo tradition and taint their so-called sacred battlefield. Critics of sumo’s female taboo denounce the Association’s rule as anachronistic and sexist as well as illegal. The opposition focuses attention on the need for change, and justifiably so, but there exists an equally pressing need to think about why the ban prevails even with strong opposition. Olympic presentations of the male sumo wrestler open our eyes as to one such abetting force: persuasive and politically expedient visions of sumo wrestling as an ancient, sacred, and exclusively male endeavor.
The Covenants of the Prophet and the Problems of Transmission: An Analysis of a Manuscript Copied by Fāris al-Shidyāq
Authors: Morrow John Andrew
Published: September 2021

This study examines a covenant of the Prophet, namely, a treaty, patent of protection or charter of privileges, that was copied by Fāris al-Shidyāq at some time before the middle of the nineteenth century. It provides a biographical sketch of the copyist. It reproduces the Arabic original as found in Majmū‘ fawā’id along with an English translation. This is followed by a commentary on the covenant and a series of conclusions, namely, that the “Shidyāq Covenant” from 1857 is a copy of the “Rylands Covenant,” which appears to be an Ottoman-issued document dating from the sixteenth or seventeenth century. This “Shidyāq/Rylands Covenant” could represent the missing link between the “Covenant of the Prophet Muḥammad with the Christians of Najrān,” found in the Chronicle of Seert, and the “Covenant of the Prophet Muḥammad with the Christians of the World,” namely, the Testamentum et Pactiones made famous by Gabriel Sionita in 1630. The significance of this study resides in the fact that it shares a previously unpublished and unstudied covenant of the Prophet Muḥammad, in both Arabic and English, with the scholarly community, while exploring the problems posed by transmission. The more covenants that are rediscovered, the better we will understand their origin, diffusion, and relationship, allowing us to better assess their authenticity. What is more, if these documents are accepted by Muslims as authentic, either in word or in spirit, they can help counter and prevent radicalization, promote moderation, and help protect minorities.
Decentralized Enterprise Risk Management Issues under Rapidly Changing Environments
Authors: Bakos Levente , Dumitrașcu Dănuț Dumitru
Published: September 2021

Under the growing complexity of manufacturing processes, supply chains, markets and stakeholder expectations, enterprise risk management (ERM) has become an extremely important, probably yet still underdeveloped, management function. Enterprise risk management theory and practice should keep pace with the changes of rapidly changing environments, through new, more adaptive approaches. The article presents some of the results of a longitudinal survey at Eastern-European manufacturing organizations made on risk management techniques. The goal of the research was the study of risk management techniques under rapidly changing environments in highly standardized industries (pharmaceutical and automotive). The research was focused on the role of human resources in handling technology-related/operational risks and to what extent a decentralized risk management is present. Multidisciplinary cooperation, the selection of the teams, communication and the decision making within the team was analysed. During our research few common risk analysis routines were identified at the studied organizations. Through an interview-based qualitative survey, possible weaknesses of common risk identification techniques were identified. The article presents three risk evaluation methods with the same features. The answers provided during the interviews indicate that risk assessment techniques are mostly centralized (coordinated by a single person/unit), linear (based on If-Then construct) and rigid, definitively not suitable when quick changes are in the organization environment.
Sliding Windows Method Based on Terrain Self-Similarity for Higher DEM Resolution in Flood Simulating Modeling
Authors: Yin Qian , Chen Ziyi , Zheng Xin , Xu Yingjun , Liu Tianxue
Published: September 2021

A digital elevation model (DEM) is a quantitative representation of terrain and an important tool for Earth science and hydrological applications. A high-resolution DEM provides accurate basic Geodata and plays a crucial role in related scientific research and practical applications. However, in reality, high-resolution DEMs are often difficult to obtain. Due to the self-similarity present within terrains, we proposed a method using the original DEM itself as a sample to expand the DEM using sliding windows method (SWM) and generate a higher resolution DEM. The main processes of SWM include downsampling the original DEM and constructing mapping sets, searching for the optimal matching, window replacement. Then, we repeat these processes with the small-scale expansion factor. In this paper, the grid resolution of the Taitou Basin was expanded from 30 to 10 m. Overall, the superresolution reconstruction results showed that the method could achieve better outcomes than other commonly used techniques and exhibited a slight deviation (root mean square error (RMSE) = 3.38) from the realistic DEM. The generated high-resolution DEM prove to be significant in the application of flood simulation modeling.
Assessing the Reliability of Satellite and Reanalysis Estimates of Rainfall in Equatorial Africa
Authors: Nicholson Sharon E. , Klotter Douglas A.
Published: September 2021

This article examines the reliability of satellite and reanalysis estimates of rainfall in the Congo Basin and over Lake Victoria and its catchment. Nine satellite products and five reanalysis products are considered. They are assessed by way of inter-comparison and by comparison with observational data sets. The three locations considered include a region with little observational gauge data (the Congo), a region with extensive gauge data (Lake Victoria catchment), and an inland water body. Several important results emerge: for one, the diversity of estimates is generally very large, except for the Lake Victoria catchment. Reanalysis products show little relationship with observed rainfall or with the satellite estimates, and thus should not be used to assess rainfall in these regions. Most of the products either overestimate or underestimate rainfall over the lake. The diversity of estimates makes it difficult to assess the factors governing the interannual variability of rainfall in these regions. This is shown by way of correlation with sea-surface temperatures, particularly with the Niño 3.4 temperatures and with the Dipole Mode Index over the Indian Ocean. Some guidance is given as to the best products to utilize. Overall, any user must establish that the is product reliable in the region studied.