bluuebunny/crossref_metadata_2025 huggingface dataset:
Selecting the ‘Sustainable’ Cow Using a Customized Breeding Index: Case Study on a Commercial UK Dairy Herd
Authors: Bell Matt J. , Jauernik Greta-Marie
Published: February 2023

The aim of the current study was to investigate using a customized profit and carbon total merit index to identify sustainable milking cows and herd replacements within a commercial dairy herd. Balancing the economic, social and environmental aspects of milk production has gained interest given the increasing global demand for milk products. Furthermore, a farm-level customized breeding index with farm-derived weightings for biological traits would incorporate the effect of the farm environment. This study used a Markov chain approach to model a commercial dairy herd in the UK between the years 2017 and 2022. Production, financial, genetic and nutritional data for the herd were used as input data. The model derived the economic (GBP per unit) and carbon values (kilograms CO2-eq. emissions per unit) for a single phenotypic increase in milk volume, milk fat yield, milk protein yield, somatic cell count, calving interval and lifespan, which were used in a profit and carbon index. The study proposed a methodology for selecting individual milking cows and herd replacements based on their potential to increase herd profitability and reduce carbon emissions as a means to identify more sustainable animals for a given farm environment. Of the 370 cows and herd replacements studied, 76% were classified as sustainable with a desirable increase in profit and reduction in carbon emissions. Customized breeding indices with trait weightings derived from the farm environment and selecting individual animals on economic and carbon metrics will bring permanent and cumulative improvements to the sustainability of milk production with appropriate nutrition and management. The approach used can be applied to any commercial farm to select animals that are more sustainable.
Global Impacts of Climate Policy and Trade Agreements on Greenhouse Gas Emissions
Authors: Balogh Jeremiás , Mizik Tamás
Published: February 2023

To limit increasing air pollution and fossil- energy production, several environmental and climate agreements have been established globally. In addition, trade agreements could also serve to achieve climate-mitigation goals, through a trade policy with environmental regulation. By removing tariffs and harmonizing standards on environmentally friendly products and eliminating distortionary subsidies on fossil-energy production, climate change can be mitigated. The objective of the research is to explore the effects of economic growth, international trade agreements and climate conventions on greenhouse gas emissions between 1990 and 2019, at the global level. As an econometric method, an air-pollution function is estimated by panel-regression models. The results confirm that global climate agreements have a significant, but only small, mitigating impact on global greenhouse-gas emissions. The results supported the inverted-U-shaped environmental Kuznets curve. In contrast, the environmental impacts of free-trade agreements had ambiguous results on emissions, as the members of the World Trade Organization contributed to the decrease in air pollution, while countries that signed the regional trade agreements were unable to limit emissions.
Impact of Different Levels of Crude Protein on Production Performance and Meat Quality in Broiler Selected for Slow Growth
Authors: Usturoi Marius Giorgi , Radu-Rusu Răzvan-Mihail , Usturoi Alexandru , Simeanu Cristina , Doliș Marius Gheorghe , Rațu Roxana Nicoleta , Simeanu Daniel
Published: February 2023

The production performance and meat quality of the slow-growing hybrid Hubbard JA757 were monitored under conditions of diets differentiated by crude protein content. A total of 1200 as-hatched day-old chickens were equally and randomly allotted into two treatments (T-1 and T-2), with six replicates provided for each treatment (100 chickens/replicate). T-1 chickens received standard diets (according to Hubbard Company recommendations), and those in T-2 were fed diets supplemented with crude protein (+0.5% CP in the growing phase and +1.0% CP in the finishing phase). At the end of the investigations (age 56 days), the T-2 chickens performed better than the T-1 chickens for growth traits (+2.72% body weight; +2.77% daily growth gain; −0.34% mortality; and −4.15% feed conversion ratio); for slaughtering (+0.66% dressed yield; +1.10% breast weight; and +1.25% thigh weight); and for quality meat (+0.55% dry matter in thigh muscles and +1.52% dry matter in breast muscles) (p > 0.05). Statistically significant differences (p < 0.05) occurred between treatments for body weight, daily weight gain, and feed conversion ratio due to the 0.5% CP feed supplementation during the 15–28 day age period, justifying the usefulness of the CP increasing throughout the grower diet only and not during the finishing period.
The Determinants of Smallholder Farmers on the Functionality of Plant Health Clinics in the Vhembe District, South Africa
Authors: Rambauli Maanda , Antwi Michael Akwasi , Tshikhudo Phumudzo Patrick , Mudau Fhatuwani Nixwell
Published: February 2023

A plant health clinic is a system in which under-resourced and smallholder farmers can access basic services of plant healthcare from a plant clinic in relation to infected or symptomatic plants and plant products for pest diagnostic and identification purposes. The factors that influence smallholder farmers’ awareness and accessibility to plant health clinics in the Vhembe District Municipality of Limpopo Province, South Africa were examined in this study. The study also identified the constraints of the plant clinics that prevent their effective utilization. Three-hundred and twenty smallholder farmers (n = 320) from the population of 1600 were interviewed through a semi-structured questionnaire. A multistage random sampling technique was employed to select the 320 smallholder farmers from 8 irrigation schemes in the study area. To ensure representation, all categories of smallholder farmers were included in the study. Descriptive statistics such as frequency and percentages and Logit regression model were employed for the data analyses. The results revealed that there is a statistically significant and positive relation between communication channels and plant clinics awareness and accessibility among smallholder farmers. The distance to plant clinics has a negative influence on the accessibility of plant clinics among the farmers; however, the accessibility of plant clinic improves when visits by extension officers or plant doctors to farmers increase. The study pointed out some challenges affecting the functionality of plant clinics, which could be resolved through an efficient and effective plant clinic framework that involves major role players. An efficient and effective plant clinic framework was therefore developed by the study to improve its usefulness for diagnosing plant pest symptoms and diseases. The study concluded that plant clinic framework was important and should be considered and adopted by policy makers within the government, research institutes, and other NGOs.
Circadian Clock Contributes to Modulate Salinity Stress-Responsive Antioxidative Mechanisms and Chloroplast Proteome in Spinacia oleracea
Authors: Venkat Ajila , Bae Dong-Won , Muneer Sowbiya
Published: February 2023

Extreme abiotic stresses such as drought, salinity, and temperature reduce crop productivity significantly and pose a serious threat to the area of land used for agriculture. Therefore, there is a pressing need to create crops that can thrive in these circumstances. It has been noted that plants can maintain defense mechanisms during any environmental changes and anticipate diurnal patterns correct to a circadian-based clock. Therefore, the main aim of this study was to investigate the role of circadian core oscillators in response to salinity stress in an important vegetable crop, spinach, and obtain evidence to better understand salinity stress adaptation for crop productivity. Therefore, the current study was carried out to examine the circadian clock-based (morning–evening loop) salinity stress defense mechanism in spinach (Spinacia oleracea), a leafy vegetable crop with significant economic importance and health benefits. In the presence of dawn and dusk, the circadian clock-based defense mechanism was observed using the genotypes “Delhi Green” and “Malav Jyoti.” A photoperiodic rhythm consists of 4-h intervals for 12 h (morning–evening loop) in spinach was demonstrated under the salinity stress treatments (20 mM and 50 mM). The clock-controlled a large fraction of growth parameters such as plant height, biomass, and root-shoot ratio under salinity stress. Conversely, salinity stress resulted in upregulation of antioxidative parameters such as superoxide dismutase, ascorbate peroxidase, catalase, and other stress markers such as thiobarbituric acid reactive substances, proline content, and localizations of H2O2 and O2−1 but was altered and maintained at a certain photoperiodic time interval of the circadian clock. In distinction to results observed from antioxidative measurements performed with an early and late circadian duration of salt-treated plants, 10 am and 2 pm were revealed to be the rhythmic times for controlling salinity stress. Likewise, comprehensive measurements of the photosynthetic system under salinity stress at specific photoperiodic circadian time intervals, including net-photosynthetic rate, transpiration, stomatal conductance, PSII quantum yield, and stomata structure, were made at 10 am and 2 pm. The salinity stress response was down-streamed and the clock also regulated chloroplastic protein expression. Thus, according to our findings, photoperiodic circadian rhythms, particularly the morning–evening loop, enhanced plant survival rates by modulating cellular antioxidant mechanisms and chloroplastic proteins that further helped to reduce the effects of salinity stress.
Combined Effects of Parsnip Fermented Juice and Hawthorn Extract Regarding Pork Mince Stability: Physico-Chemical and Microbiological Aspects
Authors: Predescu Corina Nicoleta , Papuc Camelia , Stefan Georgeta , Tașbac Bogdan , Temocico Georgeta , Sărăcilă Mihaela , Untea Arabela Elena
Published: February 2023

Parsnip fermented juice (PFJ) and hawthorn extract (HE) were identified as natural nitrite and antioxidant sources for pork mince. This study aimed to determine the effects of varying levels of HE added to a constant concentration of PFJ on lipids stability, heme pigment conversion degree, residual nitrite content, and spoilage bacteria growth, during refrigeration, compared with the combined effect of synthetic nitrite and sodium ascorbate (SA). Pork mince was formulated in six different ways with sterile distilled water (NC), 100 ppm synthetic nitrite and 50 ppm SA (PC), PFJ in the concentration of 100 ppm NO2− (T1), constant level of PFJ (100 ppm NO2−), and increased level of HE, 50, 25 and 10 ppm GAE (T2, T3 and T4). During the experiment, pH increased for all the treatments, but the addition of PFJ alone or in combination with HE, it was maintained below the NC pH value. The lowest TBARS values and the highest PUFA concentrations were found in the T3, T4, and PC treatments. Of all the samples, the lowest residual nitrite values were found for T2. The highest NO-heme values were found for T2 and PC. After 9 days of storage, TVC results were higher than 5.69 logs CFU/g for all treatments. Overall, the obtained results showed that the combination of HE and PFJ could be a promising natural preservative for minced meat that could replace synthetic preservatives.
SOLARIA-SensOr-driven resiLient and adaptive monitoRIng of farm Animals
Authors: Neethirajan Suresh
Published: February 2023

Sensor-enabled big data and artificial intelligence platforms have the potential to address global socio-economic trends related to the livestock production sector through advances in the digitization of precision livestock farming. The increased interest in animal welfare, the likely reduction in the number of animals in relation to population growth in the coming decade and the growing demand for animal proteins pose an acute challenge to prioritizing animal welfare on the one hand, while maximizing the efficiency of production systems on the other. Current digital approaches do not meet these challenges due to a lack of efficient and lack of real-time non-invasive precision measurement technologies that can detect and monitor animal diseases and identify resilience in animals. In this opinion review paper, I offer a critical view of the potential of wearable sensor technologies as a unique and necessary contribution to the global market for farm animal health monitoring. To stimulate the sustainable, digital and resilient recovery of the agricultural and livestock industrial sector, there is an urgent need for testing and developing new ideas and products such as wearable sensors. By validating and demonstrating a fully functional wearable sensor prototype within an operational environment on the livestock farm that includes a miniaturized animal-borne biosensor and an artificial intelligence (AI)-based data acquisition and processing platform, the current needs, which have not yet been met, can be fulfilled. The expected quantifiable results from wearable biosensors will demonstrate that the digitization technology can perform acceptably within the performance parameters specified by the agricultural sector and under operational conditions, to measurably improve livestock productivity and health. The successful implementation of the digital wearable sensor networks would provide actionable real-time information on animal health status and can be deployed directly on the livestock farm, which will strengthen the green and digital recovery of the economy due to its significant and innovative potential.
Spatial Correlation Evolution and Driving Factors of Wheat Production in China Based on Social Network Analysis
Authors: Lv Xinru , Zhang Han , Zhang Yunhua , Li Junli
Published: February 2023

Understanding the spatial correlation network of inter-provincial wheat production is vital for ensuring food security and achieving sustainable agricultural development in China. However, the spatial correlation characteristics of wheat production and their determinants remain unclear. In this study, an improved gravity model was used to calculate the spatial correlation of inter-provincial wheat production in China based on available panel data from 2000 to 2020. The spatial-temporal evolution characteristics and the driving factors of the spatial correlation network of inter-provincial wheat production in China were analyzed using social network analysis (SNA) and a quadratic assignment procedure (QAP). The findings indicated that (1) the spatial correlation of inter-provincial wheat production first increased and then decreased. The network density increased from its lowest value (0.2598) in 2000 to its maximum value (0.2782) in 2016 and then continued to fluctuate. (2) The spatial correlation network of inter-provincial wheat production presented a “core-periphery” distribution pattern for the major wheat-producing areas (such as Jiangsu, Anhui, and Hubei) and non- major wheat-producing areas (such as Jilin, Qinghai, Guangxi, and Beijing), and the roles of the blocks in the network varied with time and space. (3) The implementation of grain-related policies (such as the abolition of agricultural taxes, the implementation of industry nurturing agriculture, and the minimum grain purchase price policy) positively affected the development of the spatial correlation network of wheat production. Since the implementation of the minimum purchase price policy for wheat in 2006, the network density reached its maximum value (0.2782), the network efficiency reached its minimum value (0.5985), and the stability of the network structure greatly improved. (4) The interactions between the internal natural conditions and the external socioeconomic factors promoted the construction of a spatial correlation network for wheat production. The differences in geographical adjacency, land resources, temperature, and sunlight hours were all significant at the 1% level, highlighting the substantial impact of these factors on the spatial correlation intensity of wheat production in China. This study provides a reference for the development of cooperative cross-regional wheat production and the formulation of distinct policies for the production of wheat and other grains.
Prediction Model of Pumpkin Rootstock Seedlings Based on Temperature and Light Responses
Authors: Yan Zhengnan , Cheng Jie , Wan Ze , Wang Beibei , Lin Duo , Yang Yanjie
Published: February 2023

Temperature and light are the key factors that affect the quality of pumpkin rootstock seedlings’ growth process. Responses to temperature and light are an important basis for optimizing the greenhouse environment. In order to determine the quantitative effects of temperature and light on the growth and development of pumpkin (Cucurbita moschata cv. RTWM6018) rootstock seedlings, relationships between temperature, light, and pumpkin rootstock seedlings growth were established using regression analysis. The results indicated that the daily average temperature had a significant negative correlation with the development time of pumpkin rootstock seedlings, and the shoot dry weight of pumpkin rootstock seedlings increased within a certain range of the daily light integral (DLI). We established a prediction model of pumpkin rootstock seedling quality indicators (hypocotyl length, stem diameter, shoot dry weight, root dry weight, root shoot ratio, and seedling quality index) based on thermal effectiveness and photosynthetic photon flux density (TEP). The coefficient of determinations (R2) of the hypocotyl length and seedling quality index prediction models of pumpkin rootstock seedlings, based on accumulated TEP, were 0.707 and 0.834, respectively. The hypocotyl length and seedling quality index prediction models of pumpkin rootstock seedlings, based on accumulated TEP, were y1 = 0.001 x2 − 0.180 x + 13.057 and y2 = 0.008 x0.722, respectively, which could be used for predicting the growth of pumpkin rootstock seedlings grown under different temperature and light conditions.
Chitosan Reduces Damages of Strawberry Seedlings under High-Temperature and High-Light Stress
Authors: Fu Erting , Zhang Yuzheng , Li Huiling , Wang Xiaozhe , Zhang Hanxiao , Xiao Wei , Chen Xiude , Li Ling
Published: February 2023

High-temperature and high-light are the main adversity stresses faced by strawberry seedlings and open-field cultivation in summer. Chitosan (CTS) is widely used in agriculture as a soil amendment, plant growth regulator, and promoter of cold resistance. However, the effects of CTS on strawberry seedlings under the combined stress of high-temperature and high-light are unclear. This study aimed to determine the effect of CTS on strawberry seedlings under the combined stress of high-temperature and high-light. In this study, Xuelixiang strawberry was used as the experimental plant material. The leaves were sprayed with 100 mg/Kg CTS or deionized aqueous solution and placed under high-temperature and high-light (38 °C, 1800 μ mol·m−2·s−1, respectively) for 8 h. In addition, a suitable temperature and light (23 °C, 400 μ mol·m−2·s−1, respectively) was sprayed with deionized water as a control. The results showed that compared with non-CTS treatment, the chlorophyll (Chl) content in strawberry plants increased by 16.9% after CTS treatment; the net CO2 exchange (Pn) increased by 74.9%; and the maximum photochemical efficiency increased by 20.1%. In addition, CTS treatment increased the content of antioxidants; increased osmotic adjustment substances; improved the activity of reduced glutathione with reduced ascorbic acid (AsA-GSH) circulating antioxidant enzymes; removed reactive oxygen species in plants in time; and reduced the damage of reactive oxygen species to photosynthetic organs and cell membranes, thereby reducing high-temperature and high-light injury to strawberry seedlings. This study shows that CTS can improve the negative effects of high-temperature and high-light stress on strawberry seedlings.
Mechanistic Understanding of Leakage and Consequences and Recent Technological Advances in Improving Nitrogen Use Efficiency in Cereals
Authors: Yadav Malu Ram , Kumar Sandeep , Lal Milan Kumar , Kumar Dinesh , Kumar Rakesh , Yadav Rajendra Kumar , Kumar Sandeep , Nanda Gangadhar , Singh Jogendra , Udawat Pushpika , Meena Nirmal Kumar , Jha Prakash Kumar , Minkina Tatiana , Glinushkin Alexey P. , Kalinitchenko Valery P. , Rajput Vishnu D.
Published: February 2023

Although nitrogen (N) is the most limiting nutrient for agricultural production, its overuse is associated with environmental pollution, increased concentration of greenhouse gases, and several human and animal health implications. These implications are greatly affected by biochemical transformations and losses of N such as volatilization, leaching, runoff, and denitrification. Half of the globally produced N fertilizers are used to grow three major cereals—rice, wheat, and maize—and their current level of N recovery is approximately 30–50%. The continuously increasing application of N fertilizers, despite lower recovery of cereals, can further intensify the environmental and health implications of leftover N. To address these implications, the improvement in N use efficiency (NUE) by adopting efficient agronomic practices and modern breeding and biotechnological tools for developing N efficient cultivars requires immediate attention. Conventional and marker-assisted selection methods can be used to map quantitative trait loci, and their introgression in elite germplasm leads to the creation of cultivars with better NUE. Moreover, gene-editing technology gives the opportunity to develop high-yielding cultivars with improved N utilization capacity. The most reliable and cheap methods include agronomic practices such as site-specific N management, enhanced use efficiency fertilizers, resource conservation practices, precision farming, and nano-fertilizers that can help farmers to reduce the environmental losses of N from the soil–plant system, thus improving NUE. Our review illuminates insights into recent advances in local and scientific soil and crop management technologies, along with conventional and modern breeding technologies on how to increase NUE that can help reduce linked N pollution and health implications.
Promoting Strawberry (Fragaria × ananassa) Stress Resistance, Growth, and Yield Using Native Bacterial Biostimulants
Authors: Sangiorgio Daniela , Cellini Antonio , Spinelli Francesco , Donati Irene
Published: February 2023

Strawberry production is challenged by several abiotic and biotic stresses, such as drought, soil salinity, and the angular leaf spot (ALS) disease caused by Xanthomonas fragariae. In recent decades, the development of commercial products containing combinations of different Plant-Growth-Promoting (PGP) microorganisms has been one of the main focuses of agricultural research. However, their results are often erratic depending on crop species, environmental conditions, and competition among the different strains or indigenous plant microbiota. The use of beneficial microorganisms selected from the crop-specific microbiota may help overcome this limitation, promoting their utilization for sustainable agriculture. The culturable bacteriota of strawberry plants was screened to identify PGP activities in vitro. Bacterial isolates were tested in vivo on strawberry plants in both optimal and stress (X. fragariae infection or salinity) conditions, allowing the selection of strains of Pseudomonas fluorescens, Stenotrophomonas rhizophila, and Agrobacterium rubi whose application showed a significant increase in plant growth and fruit production (up to seven-fold), even under stress conditions, and the ability to control ALS by over 50%. Potential synergistic effects among PGP isolates were tested by coordinated inoculation. However, plant growth and fruit quality were not promoted, except for fruit weight and size, by coordinate inoculation in comparison to m23 and m27 single-strain treatment.
Can the Adoption of ICT and Advisory Services Be Considered as a Tool of Competitive Advantage in Agricultural Holdings? A Literature Review
Authors: Kountios Georgios , Konstantinidis Christos , Antoniadis Ioannis
Published: February 2023

Knowledge of better farming methods has been a crucial step upon which agriculture has grown over time. Knowledge, on the other hand, is a currency transferred from one person to another with the vision to improve the quality of life of the other person. Agricultural knowledge has been transferred from one generation to the next based on the experience of one society and whatever knowledge they have developed in their existence. This shows that possession of a better and deeper understanding of agricultural processes and strategies is vital in developing the agricultural sector within a particular society. Therefore, better ways of acquiring agricultural knowledge together with improved methods of transmission of the same knowledge is primary in the advancement of agriculture within a group of people. In the last years, farming and agriculture have made significant strides in utilizing Information and Communication Technology (ICT), particularly in the realm of growers’ reach to market-based knowledge. Information and communication technology is being incorporated in several interventions that aim to properly educate producers about agriculture. In this context, ICT also helps them hold a competitive advantage in the process. This article addresses numerous cutting-edge initiatives that use Information and communication technology’s purpose of providing agricultural knowledge to farmers. Its assessment focuses primarily on answering the question of the intensity of change brought about by ICT and advisory services in agriculture. This focus enables this review to give a clear and conclusive view of how the two factors are creating a competitive advantage amongst different farmer groups and localities.
Comparison of the Efficacy of Trichoderma and Bacillus Strains and Commercial Biocontrol Products against Grapevine Botryosphaeria Dieback Pathogens
Authors: Langa-Lomba Natalia , González-García Vicente , Venturini-Crespo M. Eugenia , Casanova-Gascón José , Barriuso-Vargas Juan J. , Martín-Ramos Pablo
Published: February 2023

Grapevine trunk diseases (GTDs) cause significant yield losses worldwide and limit the lifespan of vineyards. In the last few years, using biological control agents (BCAs) for pruning wound protection has become a promising management strategy for the control of these pathologies. This study aimed to compare the antifungal activities of a grapevine-native Trichoderma harzianum isolate and a high-potential Bacillus velezensis strain against two pathogenic Botryosphaeriaceae species in artificially inoculated, potted, grafted plants under controlled greenhouse conditions, taking three commercial biocontrol products (based on T. atroviride I-1237, T. harzianum T-22, and Bacillus subtilis BS03 strains) as a reference. To reproduce certain field conditions more realistically, inoculation of the protective agents and the pathogens was conducted simultaneously immediately after pruning instead of allowing the BCAs to colonize the wounds before pathogen inoculation. Significant differences in necrosis lengths were detected for both Neofusicoccum parvum- and Diplodia seriata-infected plants, and a remarkable protective effect of Bacillus velezensis BUZ-14 was observed in all cases. Trichoderma-based treatments showed different efficacies against the two pathogenic fungi. While the three tested BCAs resulted in significant reductions in vascular necrosis caused by N. parvum, they did not significantly reduce D. seriata infection compared to the untreated inoculated control. The B. subtilis strain was not effective. The reported results provide support for the potential Bacillus velezensis may have for pruning wound protection against Botryosphaeriaceae fungi, encouraging its evaluation under natural field conditions.
Greater Propensity to Photosynthesize Enables Superior Grain Quality of Indica–japonica Hybrid Rice under Shading
Authors: Shang Cheng , Harrison Matthew Tom , Deng Jun , Ye Jiayu , Zhong Xuefen , Wang Chunhu , Tian Xiaohai , Huang Liying , Liu Ke , Zhang Yunbo
Published: February 2023

Indica–japonica hybrid rice (I–JR) typically has greater grain yield than that of Indica hybrid rice (IR) under prolific shading, but it is not known how shading impacts on physiological characteristics underpinning grain quality. Here, we conducted a two-year field experiment in the mid-reaches of the Yangtze River region using I–JR (genotypes Yongyou 1540 and Yongyou 538) and IR (genotypes Y-liangyou 900 and Quanyouhuazhan). We found that shading reduced grain appearance and quality, particularly milling and heading rates, and chalkiness. Shading disrupted carbon and nitrogen metabolism, impacting traits influencing the human perception of the taste of the grain, such that amylose decreased by 5.9% (I–JR) and 12.9% (IR); grain protein significantly increased, with lesser effects in I–JR than IR under shading. Shading also reduced peak, hot, and final viscosities, and breakdown value. I–JR had improved rice quality compared with that of IR due to the greater propensity of the former to photosynthesize under shading, leading to the improved functioning of carbon and nitrogen metabolism.
Genetic Divergence of Thai Indigenous Pigs from Three Distinct Geographic Regions Revealed by Microsatellite Marker Analysis
Authors: Chaweewan Kamon , Mahinchai Prapas , Kongsook Sornchai , Soponchit Surasak , Weerasamith Phuree , Awiruttapanich Wiranphat , Prapawat Pakhawan , Jamparat Warocha , Chanthaworn Thitawat , Rattanamahavichai Natinee , Weangchanok Sarisa , Arikit Siwaret , Duangjinda Monchai , Tuntivisoottikul Kunya , Chaosap Chanporn , Jirajaroenrat Kanya
Published: February 2023

Thai indigenous pigs (TIPs) are important genetic resources. Crosses with exotic pig breeds and wild boars may cause genetic losses. To date, the physical characteristics of TIPs have been inconsistent. The classification of TIPs by genetic information is needed to pursue an appropriate conservation program. In this study, the genetic diversity, cluster analysis, and phylogenetic relationship of TIPs were investigated using twenty-nine pig microsatellite markers. Blood samples were collected from TIPs from three regions of Thailand: north (NT, n = 118), northeast (NE, n = 61), and south (ST, n = 75). The mean total number of distinct alleles and the effective number of alleles per locus were 11.851 and 5.497, respectively. The mean observed heterozygosity (Ho) and mean expected heterozygosity (He) were 0.562 and 0.837, respectively. The F values of the microsatellite loci were positive under Hardy–Weinberg Equilibrium at p < 0.001, with overall mean values of Fis, Fit, and Fst of 0.247, 0.281, and 0.046, respectively. A total of 5, 5, and 17 private alleles were found at frequencies greater than 0.050 in the NT, NE, and ST pigs, respectively. Three optimal clusters (K = 3) were proposed within the TIP populations. Pigs from the NT and NE regions were mixed in two clusters, while members of the ST region were clearly separated. The phylogenetic tree confirmed that the pigs from NT and NE were each divided into two subgroups, while the pigs from ST were clustered into one group. A microsatellite analysis revealed the high genetic diversity of the TIP populations and confirmed the genetic divergence of the TIPs from the European and Chinese breeds. A genetic admixture of the TIP with the local wild boars was detected.
Improving Genomic Prediction Accuracy in the Chinese Holstein Population by Combining with the Nordic Holstein Reference Population
Authors: Zhang Zipeng , Shi Shaolei , Zhang Qin , Aamand Gert P. , Lund Mogens S. , Su Guosheng , Ding Xiangdong
Published: February 2023

The size of the reference population is critical in order to improve the accuracy of genomic prediction. Indeed, improving genomic prediction accuracy by combining multinational reference populations has proven to be effective. In this study, we investigated the improvement of genomic prediction accuracy in seven complex traits (i.e., milk yield; fat yield; protein yield; somatic cell count; body conformation; feet and legs; and mammary system conformation) by combining the Chinese and Nordic Holstein reference populations. The estimated genetic correlations between the Chinese and Nordic Holstein populations are high with respect to protein yield, fat yield, and milk yield—whereby these correlations range from 0.621 to 0.720—and are moderate with respect to somatic cell count (0.449), but low for the three conformation traits (which range from 0.144 to 0.236). When utilizing the joint reference data and a two-trait GBLUP model, the genomic prediction accuracy in the Chinese Holsteins improves considerably with respect to the traits with moderate-to-high genetic correlations, whereas the improvement in Nordic Holsteins is small. When compared with the single population analysis, using the joint reference population for genomic prediction in younger animals, results in a 2.3 to 8.1 percent improvement in accuracy. Meanwhile, 10 replications of five-fold cross-validation were also implemented in order to evaluate the performance of joint genomic prediction, thereby resulting in a 1.6 to 5.2 percent increase in accuracy. With respect to joint genomic prediction, the bias was found to be quite low. However, for traits with low genetic correlations, the joint reference data do not improve the prediction accuracy substantially for either population.
Integrating Reference Intervals into Chimpanzee Welfare Research
Authors: Whitham Jessica C. , Hall Katie , Lauderdale Lisa K. , Bryant Jocelyn L. , Miller Lance J.
Published: February 2023

Animal welfare researchers are committed to developing novel approaches to enhance the quality of life of chimpanzees living in professional care. To systematically monitor physical, mental, and emotional states, welfare scientists highlight the importance of integrating non-invasive, animal-based welfare indicators. This study aimed to create species-specific reference intervals for behavioral measures and physiological biomarkers. Specifically, we analyzed data from 40 adult chimpanzees (22 females, 18 males) residing at 16 zoological facilities to generate reference intervals for behavioral states and events, behavioral diversity, fecal glucocorticoid metabolites (GCMs), and fecal immunoglobulin-A (IgA). Comparisons of sex and age using linear regression models revealed significant differences for several behaviors. The proportion of time spent engaged in mutual/multiple social grooming significantly decreased as individuals aged. Furthermore, males spent a higher proportion of time performing aggressive contact behaviors and displaying to other chimpanzees when compared to females. Males also performed sexual examination behaviors at a higher rate than females. Behavioral diversity, fecal GCM, and fecal IgA did not vary by sex or age. In the future, values for individual chimpanzees can be compared to the ranges reported here for particular age/sex classes. Ultimately, animal care professionals can utilize reference intervals to make evidence-based decisions regarding management practices and environmental conditions.
The Effectiveness of Molecular, Karyotype and Morphological Methods in the Identification of Morphologically Conservative Sibling Species: An Integrative Taxonomic Case of the Crocidura attenuata Species Complex in Mainland China
Authors: Li Haotian , Li Yaoyao , Motokawa Masaharu , Wu Yi , Harada Masashi , Li Yuchun
Published: February 2023

The conservation of morphology has resulted in considerable issues in the taxonomy of small mammals, especially for the identification of sibling species. Moreover, it is often difficult to completely solve such taxonomic problems by relying only on a single research method. The genus Crocidura is one of the genera with a conservative morphology and high species diversity. Among them, Crocidura attenuata has been considered in the field as the most widely distributed and common species. In fact, it is a species complex containing multiple species, and the classification and distribution of this species is controversial. In this study, the species and distribution of the Crocidura attenuata species complex experienced an integrated revision using three different levels of research methods: molecular, karyotype and morphology. The results show that (1) the C. attenuata species complex contains four known species (C. attenuata, C. tanakae, C. anhuiensis and C. dongyangjiangensis) and a cryptic species distributed in Guangxi, which may be the same undescribed species as the “C. attenuata” distributed in Vietnam. (2) C. attenuata is only distributed around the Sichuan Basin, C. tanakae is the most widely distributed throughout Southern China, and C. anhuiensis and C. dongyangjiangensis are almost sympatric in Southeast China. Furthermore, (3) although the molecular method lacks a unified threshold for species classification, it can rapidly and effectively identify the species of the C. attenuata species complex. Although karyotype and morphology methods cannot completely solve the species classification issues in respect of the C. attenuata species complex, they can provide supplemental information for taxonomic purposes. Therefore, the integrated taxonomic method can present the advantages of different methodological levels, and will provide further evidence for the taxonomy of sibling species with a conservative morphology.
Effect of the Supplementation Using an Herbal Mixture as a Choline Source during Early Gestation in Rambouillet Ewes
Authors: Suárez-Suárez Nydia Emilce , Lee-Rangel Héctor Aaron , Lizarazo-Chaparro Augusto César , Mendoza-Martínez German David , Espinosa-Reyes Guillermo , Hernández-García Pedro Abel , García-López Juan Carlos , Martínez-García José Antonio , Álvarez-Fuentes Gregorio , Roque-Jiménez José Alejandro
Published: February 2023

Previous research indicates that adequate choline nutrition during late gestation improves fetal development. However, there is a lack of studies describing choline’s role during early gestation. Thus, the current study hypothesizes that an herbal mixture as a source of choline (Biocholine) positively affects offspring development from ewes supplemented during early gestation. Therefore, the objectives were to evaluate the impact of biocholine on the programming of the offspring early in life through the evaluation of dams and newborn performance. Twenty-eight four-year-old Rambouillet ewes were assigned randomly to two treatments: non-supplementation and 4 gd−1 of biocholine during the early gestation. Compared with the dams without supplementation, the ewes supplemented using biocholine showed no increase in parameters such as birth and weaning weight (p > 0.05). Additionally, the milk yield and quality of colostrum and milk did not present statistical differences (p > 0.05). However, the placental membrane development was reduced in the ewes that received supplementation with biocholine; interestingly, those dams increased the weight of the newborns during the lambing period (p < 0.05). Finally, the current study proposes necessary elucidation of how placental size is programmed and if less placental development has potential benefits in the fetus’s development.
Serum Oxytocin, Cortisol and Social Behavior in Calves: A Study in the Impossible Task Paradigm
Authors: Pinelli Claudia , Scandurra Anna , Mastellone Vincenzo , Iommelli Piera , Musco Nadia , Pero Maria Elena , Di Lucrezia Alfredo , Lotito Daria , Tudisco Raffaella , D’Aniello Biagio , Infascelli Federico , Lombardi Pietro
Published: February 2023

In this study, we explored the correlations between circulating levels of oxytocin, cortisol, and different social behaviors toward humans in 26 Italian Red Pied calves (all females, with an average age of 174 ± 24 days) using the impossible task paradigm. This paradigm has proved fruitful in highlighting the effect of socialization on the willingness to interact with humans in several domesticated species. The test consists of the violation of an expectation (recovering food from an experimental apparatus) while a caregiver and a stranger are present. Immediately after the end of the test (less than one minute), blood was collected from the coccygeal vein. Statistics were performed by the Spearman’s rank correlation; significant differences were adjusted according to Bonferroni’s correction. Cortisol correlates positively (ρ = 0.565; p < 0.05) with the latency of behaviors directed at the caregiver, and the duration of behaviors directed at the apparatus correlates negatively with both the caregiver (ρ = −0.654; p < 0.05) and a stranger (ρ = −0.644; p < 0.05). Contrary to what is reported in the literature on cows, no correlations were found between oxytocin levels and direct behaviors toward the caregiver. This highlights a different behavioral strategy between calves and cows when placed in front of an impossible task.
Heavy Metal(loid) Accumulation in the Ovarian Tissue of Free-Ranging Queens and Bitches Inhabiting Highly Polluted Urban Environments
Authors: Forte Giovanni , Ariu Federica , Bocca Beatrice , Solinas Giuliana , Leoni Giovanni Giuseppe , Podda Andrea , Madeddu Roberto , Bogliolo Luisa
Published: February 2023

There is strong scientific evidence that exposure to environmental contaminants, such as heavy metal(loid)s (HMs), can impair female reproductive function. Pets, such as cats and dogs, who share the same habitat as humans, may be particularly useful sentinel models for detecting HMs in the ovary. In the present study, we compared the concentration of essential (Ems; Cu, Fe, Mn, Se, and Zn) and non-essential metal(loid)s (NEMs; Al, As, Cd, and Pb) in the ovarian tissues of free-ranging queens and bitches of different ages living in industrialized/highly polluted (south group) and non-polluted (north group) urban areas of the island of Sardinia, Italy. The results showed that both EMs and NEMs were present at detectable concentrations in feline and canine ovaries and their levels varied according to geographical areas and animal age. Among the EMs, Cu was found elevated in older queens and bitches inhabiting the southern area. Cadmium and lead were higher in feline and canine ovaries of older animals from the south compared to those living in the north. In addition, Cd and Pb concentrations increased in individuals of both species living in the south. These findings showed new perspectives for the use of pets as early warning sentinels of environmental pollution by HMs and for the risk of human exposure within a “One Health” approach. Pets may help to study the link between exposure to metals and female reproductive disturbances in mammals.
Comparative Genomics Identifies Novel Genetic Changes Associated with Oxacillin, Vancomycin and Daptomycin Susceptibility in ST100 Methicillin-Resistant Staphylococcus aureus
Authors: Di Gregorio Sabrina , Haim María Sol , Famiglietti Ángela María Rosa , Di Conza José , Mollerach Marta
Published: February 2023

Infections due to vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA) represent a serious concern due to their association with vancomycin treatment failure. However, the underlying molecular mechanism responsible for the hVISA/VISA phenotype is complex and not yet fully understood. We have previously characterized two ST100-MRSA-hVISA clinical isolates recovered before and after 40 days of vancomycin treatment (D1 and D2, respectively) and two in vitro VISA derivatives (D23C9 and D2P11), selected independently from D2 in the presence of vancomycin. This follow-up study was aimed at further characterizing these isogenic strains and obtaining their whole genome sequences to unravel changes associated with antibiotic resistance. It is interesting to note that none of these isogenic strains carry SNPs in the regulatory operons vraUTSR, walKR and/or graXRS. Nonetheless, genetic changes including SNPs, INDELs and IS256 genomic insertions/rearrangements were found both in in vivo and in vitro vancomycin-selected strains. Some were found in the downstream target genes of the aforementioned regulatory operons, which are involved in cell wall and phosphate metabolism, staphylococcal growth and biofilm formation. Some of the genetic changes reported herein have not been previously associated with vancomycin, daptomycin and/or oxacillin resistance in S. aureus.
Overruling of Procalcitonin-Guided Antibiotics for Lower Respiratory Tract Infections in Primary Care: Ancillary Study of a Randomized Controlled Trial
Authors: Knüsli José , Lhopitallier Loïc , Kronenberg Andreas , Meuwly Jean-Yves , Opota Onya , Perrenoud Marc-Antoine , Page Marie-Anne , Kain Kevin C. , Mamin Aline , D’Acremont Valérie , Senn Nicolas , Mueller Yolanda , Locatelli Isabella , Boillat-Blanco Noémie
Published: February 2023

Background: Lower respiratory tract infections (LRTIs) in primary care are a promising target for antibiotic stewardship. A clinical trial in Switzerland showed a large decrease in antibiotic prescriptions with procalcitonin guidance (cut-off < 0.25 µg/L) compared with usual care. However, one-third of patients with low procalcitonin at baseline received antibiotics by day 28. Aim: To explore the factors associated with the overruling of initial procalcitonin guidance. Design and Setting: Secondary analysis of a cluster randomized trial in which patients with an LRTI were included. Method: Using the characteristics of patients, their disease, and general practitioners (GPs), we conducted a multivariate logistic regression, adjusted for clustering. Results: Ninety-five out of 301 (32%) patients with low procalcitonin received antibiotics by day 28. Factors associated with an overruling of procalcitonin guidance were: a history of chest pain (adjusted OR [aOR] 1.81, 95% confidence interval 1.03–3.17); a prescription of chest X-ray by the GP (aOR 4.65, 2.32–9.34); a C-reactive protein measured retrospectively above 100 mg/L (aOR 7.48, 2.34–23.93, reference ≤ 20 mg/L); the location of the GP practice in an urban setting (aOR 2.27, 1.18–4.37); and the GP’s number of years of experience (aOR per year 1.05, 1.01–1.09). Conclusions: Overruling of procalcitonin guidance was associated with GPs’ socio-demographic characteristics, pointing to the general behavioral problem of overprescription by physicians. Continuous medical education and communication training might support the successful implementation of procalcitonin point-of-care tests aimed at antibiotic stewardship.