bluuebunny/crossref_metadata_2025 huggingface dataset:
Monitoring Tumor Targeting and Treatment Effects of IRDye 800CW and GX1-Conjugated Polylactic Acid Nanoparticles Encapsulating Endostar on Glioma by Optical Molecular Imaging
Authors: Li Yaqian , Du Yang , Liu Xia , Zhang Qian , Jing Lijia , Liang Xiaolong , Chi Chongwei , Dai Zhifei , Tian Jie
Published: March 2018

Molecular imaging used in cancer diagnosis and therapeutic response monitoring is important for glioblastoma (GBM) research. Antiangiogenic therapy currently is one of the emerging approaches for GBM treatment. In this study, a multifunctional nanoparticle was fabricated that can facilitate the fluorescence imaging of tumor and deliver a therapeutic agent to the tumor region in vivo and therefore possesses broad application in cancer diagnosis and treatment. This particle was polylactic acid (PLA) nanoparticles encapsulating Endostar, which was further conjugated with GX1 peptide and the near-infrared (NIR) dye IRDye 800CW (IGPNE). We demonstrated noninvasive angiogenesis targeting and therapy of IGPNE on U87MG xenografts in vivo using dual-modality optical molecular imaging including NIR fluorescence molecular imaging (FMI) and bioluminescence imaging (BLI). The NIR FMI results demonstrated that IGPNE had more accumulation to the tumor site compared to free IRDye 800CW. To further evaluate the antitumor treatment efficacy of IGPNE, BLI and immunohistochemistry analysis were performed on tumor-bearing mice. With the aid of molecular imaging, the results confirmed that IGPNE enhanced antitumor treatment efficacy compared to free Endostar. In conclusion, IGPNE realizes real-time imaging of U87MG tumors and improves the antiangiogenic therapeutic efficacy in vivo.
Detection of Apoptotic Cells in a Rabbit Model with Atherosclerosis-Like Lesions Using the Positron Emission Tomography Radiotracer [18F]ML-10
Authors: Hyafil Fabien , Tran-Dinh Alexy , Burg Samuel , Leygnac Sébastien , Louedec Liliane , Milliner Milan , Ben Azzouna Rana , Reshef Ayelet , Ben Ami Miri , Meilhac Olivier , Le Guludec Dominique
Published: March 2018

[18F]ML-10 (2-(5-fluoro-pentyl)-2-methylmalonic acid) is a positron emission tomography (PET) radiotracer that accumulates in cells presenting apoptosis-specific membrane alterations. The aim of this study was to test whether [18F]ML-10 allows for the detection of apoptotic cells located in atherosclerotic plaques in rabbits. Atherosclerotic plaques were induced in the aortas of five rabbits, and five additional rabbits were used as controls. Activity in the aortas was quantified in vivo and ex vivo. The localization of [18F]ML-10 to the aortic wall was identified by autoradiography. Average target to background ratios measured in vivo by PET were higher in the aortas of atherosclerotic rabbits compared with those of control rabbits (2.00 ± 0.52 vs 1.22 ± 0.30; p < .05). Differences in [18F]ML-10 uptake between atherosclerotic and control aortas were confirmed ex vivo by PET and gamma counting (23.9 ± 11.2 vs 1.1 ± 2.4 counts/pixel; p <.05; 3.6 ± 2.0 vs 0.05 ± 0.05 % of injected activity/g; p < .05, respectively). Strong correlation was observed between the accumulation of [18F]ML-10 in aortic segments as detected by autoradiography and the number of apoptotic cells on corresponding histologic sections ( r2 = .75; p < .05). In this study, we found that atherosclerotic plaques rich in apoptotic cells can be detected with [18F]ML-10 and PET.
PIXSIC: A Wireless Intracerebral Radiosensitive Probe in Freely Moving Rats
Authors: Balasse Laure , Maerk Julia , Pain Frédéric , Genoux Aurelie , Fieux Sylvain , Lefebvre Françoise , Morel Christian , Gisquet-Verrier Pascale , Lanièce Philippe , Zimmer Luc
Published: March 2018

The aim of this study was to demonstrate the potential of a wireless pixelated β+-sensitive intracerebral probe (PIXSIC) for in vivo positron emission tomographic (PET) radiopharmacology in awake and freely moving rodents. The binding of [11C]raclopride to D2 dopamine receptors was measured in anesthetized and awake rats following injection of the radiotracer. Competitive binding was assessed with a cold raclopride injection 20 minutes later. The device can accurately monitor binding of PET ligands in freely moving rodents with a high spatiotemporal resolution. Reproducible time-activity curves were obtained for pixels throughout the striatum and cerebellum. A significantly lower [11C]raclopride tracer-specific binding was observed in awake animals. These first results pave the way for PET tracer pharmacokinetics measurements in freely moving rodents.
Evaluation of Glucose Uptake in Normal and Cancer Cell Lines by Positron Emission Tomography
Authors: Maddalena Francesca , Lettini Giacomo , Gallicchio Rosj , Sisinni Lorenza , Simeon Vittorio , Nardelli Anna , Venetucci Angela Assunta , Storto Giovanni , Landriscina Matteo
Published: March 2018

To date, there is no definitive demonstration of the utility of positron emission tomography (PET) in studying glucose metabolism in cultured cell lines. Thus, this study was designed to compare PET to more standardized methods for the quantitative assessment of glucose uptake in nontransformed and transformed living cells and to validate PET for metabolic studies in vitro. Human colon and breast carcinoma cell lines and mouse embryo fibroblasts were evaluated for [18F]fluorodeoxyglucose ([18F]FDG) uptake by PET and autoradiography and 2-deoxyglucose (2-DG) incorporation by colorimetric assay and analyzed for the radiotoxic effects of [18F]FDG and the expression levels of glucose transporters. Indeed, [18F]FDG incorporation on PET was comparable to [18F]FDG uptake by autoradiography and 2-DG incorporation by colorimetric assay, although radiotracer-based methods exhibited more pronounced differences between individual cell lines. As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. Notably, [18F]FDG incorporation resulted in low apoptotic rates, with fibroblasts being slightly more sensitive to radiotracer-induced cell death. The quantitative analysis of [18F]FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines.
Emerging Applications for Optically Enabled Intravital Microscopic Imaging in Radiobiology
Authors: Maeda Azusa , Kulbatski Iris , DaCosta Ralph S.
Published: March 2018

Radiation therapy is an effective cancer treatment used in over 50% of cancer patients. Preclinical research in radiobiology plays a major role in influencing the translation of radiotherapy-based treatment strategies into clinical practice. Studies have demonstrated that various components of tumors and their microenvironments, including vasculature, immune and stem cells, and stromal cells, can influence the response of solid tumors to radiation. Optically enabled imaging techniques used in experimental animal models of cancer offer a unique and powerful way to quantitatively track spatiotemporal changes in these tumor components in vivo at macro-, meso-, and microscopic resolutions following radiotherapy. In this review, we discuss the role of both well-established and emerging intravital microscopy techniques for studying tumors and their microenvironment in vivo, in response to irradiation. The development and application of new animal models, small animal microirradiation technologies, and multimodal optically enabled intravital microscopy techniques are emphasized within the framework of preclinical radiobiology research. We also comment on the potential influence that these newer imaging techniques may have on the clinical translation of new preclinical radiobiology discoveries.
Imaging the Met Receptor Tyrosine Kinase (Met) and Assessing Tumor Responses to a Met Tyrosine Kinase Inhibitor in Human Xenograft Mouse Models with a [99mTc] (AH-113018) or CY 5** (AH-112543) Labeled Peptide
Authors: Jagoda Elaine M. , Bhattacharyya Sibaprasad , Kalen Joseph , Riffle Lisa , Leeder Avrum , Histed Stephanie , Williams Mark , Wong Karen J. , Xu Biying , Szajek Lawrence P. , Elbuluk Osama , Cecchi Fabiola , Raffensperger Kristen , Golla Meghana , Bottaro Donald P. , Choyke Peter
Published: March 2018

Developing an imaging agent targeting the hepatocyte growth factor receptor protein (Met) status of cancerous lesions would aid in the diagnosis and monitoring of Met-targeted tyrosine kinase inhibitors (TKIS). A peptide targeting Met labeled with [99mTc] had high affinity in vitro (Kd = 3.3 nM) and detected relative changes in Met in human cancer cell lines. In vivo [99mTc]-Met peptide (AH-113018) was retained in Met-expressing tumors, and high-expressing Met tumors (MKN-45) were easily visualized and quantitated using singlephoton emission computed tomography or optical imaging. In further studies, MKN-45 mouse xenografts treated with PHA 665752 (Met TKI) or vehicle were monitored weekly for tumor responses by [99mTc]-Met peptide imaging and measurement of tumor volumes. Tumor uptake of [“mTc]-Met peptide was significantly decreased as early as 1 week after PHA 665752 treatment, corresponding to decreases in tumor volumes. These results were comparable to Cy5**-Met peptide (AH-112543) fluorescence imaging using the same treatment model. [99mTc] or Cy5**-Met peptide tumor uptake was further validated by histologic (necrosis, apoptosis) and immunoassay (total Met, p Met, and plasma shed Met) assessments in imaged and nonimaged cohorts. These data suggest that [99mTc] or Cy5**-Met peptide imaging may have clinical diagnostic, prognostic, and therapeutic monitoring applications.
Determination of Fatty Acid Metabolism with Dynamic [11C]Palmitate Positron Emission Tomography of Mouse Heart In Vivo
Authors: Li Yinlin , Huang Tao , Zhang Xinyue , Zhong Min , Walker Natalie N. , He Jiang , Berr Stuart S. , Keller Susanna R. , Kundu Bijoy K.
Published: March 2018

The goal of this study was to establish a quantitative method for measuring fatty acid (FA) metabolism with partial volume (PV) and spill-over (SP) corrections using dynamic [11C]palmitate positron emission tomographic (PET) images of mouse heart in vivo. Twenty-minute dynamic [11C]palmitate PET scans of four 18- to 20-week-old male C57BL/6 mice under isoflurane anesthesia were performed using a Focus F-120 PET scanner. A model-corrected blood input function, by which the input function with SP and PV corrections and the metabolic rate constants (k1–k5) are simultaneously estimated from the dynamic [11C]palmitate PET images of mouse hearts in a four-compartment tracer kinetic model, was used to determine rates of myocardial fatty acid oxidation (MFAO), myocardial FA esterification, myocardial FA use, and myocardial FA uptake. The MFAO thus measured in C57BL/6 mice was 375.03 ± 43.83 nmol/min/g. This compares well to the MFAO measured in perfused working C57BL/6 mouse hearts ex vivo of about 350 nmol/g/min and 400 nmol/min/g. FA metabolism was measured for the first time in mouse heart in vivo using dynamic [11C]palmitate PET in a four-compartment tracer kinetic model. MFAO obtained with this model was validated by results previously obtained with mouse hearts ex vivo.
Investigating the Relationship between Transverse Relaxation Rate (R2) and Interecho Time in MagA-Expressing, Iron-Labeled Cells
Authors: Lee Casey Y. , Thompson R. Terry , Prato Frank S. , Goldhawk Donna E. , Gelman Neil
Published: March 2018

Reporter gene–based labeling of cells with iron is an emerging method of providing magnetic resonance imaging contrast for long-term cell tracking and monitoring cellular activities. This report investigates 9.4 T nuclear magnetic resonance properties of mammalian cells overexpressing MagA, a putative iron transport protein from magnetotactic bacteria. MagA-expressing MDA-MB-435 cells were cultured in the presence and absence of iron supplementation and compared to the untransfected control. The relationship between the transverse relaxation rate ( R2) and interecho time was investigated using the Carr-Purcell-Meiboom-Gill sequence. This relationship was analyzed using a model based on water diffusion in weak magnetic field inhomogeneities (Jensen-Chandra model) as well as a fast-exchange model (Luz-Meiboom model). Increases in R2 with increasing interecho time were larger in the iron-supplemented, MagA-expressing cells compared to other cells. The dependence of R2 on interecho time in these iron-supplemented, MagA-expressing cells was better represented by the Jensen-Chandra model compared to the Luz-Meiboom model, whereas the Luz-Meiboom model performed better for the remaining cell types. Our findings provide an estimate of the distance scale of microscopic magnetic field variations in MagA-expressing cells, which is thought to be related to the size of iron-containing vesicles.
Two-year Effects of Chlorhexidine-containing Adhesives on the In Vitro Durability of Resin-dentin Interfaces and Modeling of Drug Release
Authors: Malaquias P , Gutierrez MF , Hass V , Stanislawczuk R , Bandeca MC , Arrais CAG , Farago PV , Reis A , Loguercio AD
Published: March 2018

Objectives:

To evaluate the effects of addition of diacetate chlorhexidine (CHX) at different concentrations into two etch-and-rinse adhesive systems on CHX release, as well as the immediate (IM) and two-year (2-Y) resin-dentin microtensile bond strength (μTBS) and nanoleakage (NL).

Methods:

CHX was added to XP Bond (XP) and Ambar (AM) at concentrations of 0.0 wt% (control); 0.01 wt%; 0.05 wt%; and 0.1 to 0.2 wt%. To assess the cumulative CHX release, adhesive disks were made in a metallic matrix and after light-curing were stored in water. Ultraviolet spectrophotometric measurements of the storage solution were performed to examine the release kinetics of CHX. For μTBS and NL, the occlusal enamel of molars was removed and the adhesives were applied to the dentin surface after acid etching. After composite resin build-up, specimens were sectioned to obtain μTBS sticks. The specimens were subjected to μTBS and NL at IM and after 2-Y. In addition, specimens underwent examination for CHX using micro–Raman spectroscopy. All data were submitted to statistical analysis (α=0.05).

Results:

With regard to CHX release, AM showed a slower and gradual release of CHX while XP released CHX more quickly (p<0.05), and CHX was still present in the hybrid layers after 2-Y. Both adhesives showed CHX release at 2-Y water storage. Both CHX-containing adhesives showed higher μTBS values than did the control group (p<0.05).
Objetos de Aprendizaje digital para personas con discapacidad visual en estructuras de datos: grafos (OAGRAF) / Digital Learning Objects for people with visual disabilities in data structures: graphs (OAGRAF)
Authors: Archundia Sierra Etelvina , Cerón Garnica Carmen
Published: March 2018

El método ADDIE (integrado por etapas de análisis, diseño, desarrollo, implementación y evaluación) y la propuesta que integra el modelo instruccional de los cuatro componentes (Modelo 4C/ID) en los Objetos de Aprendizaje (OA) para las estructuras de datos (grafos y del algoritmo de recorrido denominado Dijkstra), permiten a la persona con discapacidad visual incorporarse al estudio de la disciplina computacional en educación superior mediante los recursos multimedia. El presente trabajo muestra un prototipo de OA implementado en la plataforma virtual MOODLE, utilizando recursos multimedia y la herramienta de eXeLearning que le permiten a los estudiantes en Ciencias de la Computación identificar los elementos necesarios en el algoritmo de Dijkstra y su aplicación en una alternativa de aprendizaje mediante el uso de la tecnología. Las pruebas de usabilidad en lo referente a la facilidad de uso, identificación de elementos y tipos de grafos, se consideró entre las categorías de muy bien y excelente. La facilidad de aplicar el algoritmo de Dijkstra obtuvo bien en desarrollo del recorrido.
Cipo y la monarquía en Metamorfosis de Ovidio
Authors: Mollo Brisco María Eugenia
Published: March 2018

Nuestro propósito en este trabajo es analizar cómo el episodio de Cipo en el libro XV de Metamorfosis de Ovidio da forma literaria a una crisis de la experiencia: el fin de la República y el pasaje a una nueva forma de gobierno, el Principado. Tomaremos como marco teórico el concepto de “estructura del sentir” de Raymond Williams que se define como la expresión de la interacción entre el “carácter social dominante de un período” y la “experiencia real”. Nosotros postulamos que el episodio de Cipo señala un conflicto entre una idea dominante, la visión negativa de la figura del rey, y un nuevo sentimiento, la posibilidad de un rey con virtus.
Dos Romas futuras: Troya y Roma en Eneida 8 y Farsalia 9
Authors: Vizzotti Martín
Published: March 2018

La memoria fue un concepto central dentro de la configuración colectiva de la antigua Roma. La civilización romana ha sido la gran configuradora e instauradora de distintas formas de memoria, que han operado dentro del proceso de construcción y legitimación cultural de Occidente por 20 siglos. Toda creación de un lieu de mémoire implica, necesariamente, la abolición o desplazamiento de otro o, al menos, el desplazamiento semántico de las memorias asociadas a ese lugar específico. Augusto tuvo la difícil tarea de presentarse y legitimarse a sí mismo dentro de un complejo proceso histórico como la continuidad aparente de un sistema político y social perimido cuando en verdad encarnaba una nueva manera de concebir y ejercer el poder que había aprendido, a partir de la experiencia de Julio César, a presentarse de modos mucho más aprehensibles y, lo que es más relevante, asimilables para el pueblo romano.

Virgilio lleva a cabo en Eneida 8 una operación literaria de resignificación del espacio, y del tiempo, tan radical como simple. La visión virgiliana aúna operaciones poéticas ideológicas y culturales donde el poder perpetuador de la palabra configura de manera efectiva en el espacio, en este caso, una Roma aeterna cuyo pasado, resignificado en su momento primigenio, legitima no una profundidad y una gloria pasadas sino una gloria futura sine fine que es confutada por Lucano con la descripción del paseo de Cesar sobre las ruinas de Troya en el libro 9.950-999, donde Troya es representada como el mnemotopo admonitorio por excelencia del destino final de Roma.
MORPHOLOGY AND SKELETAL DEVELOPMENT OF RAT (Rattus norvegicus) FETUS AFTER FED DIETS CONTAINING Calliandra calothyrsus LEAF DURING GESTATION PERIOD
Authors: Christiani Ruth Ellisa , Setyawati Iriani , Yulihastuti Dwi Ariani
Published: March 2018

This study aims to determine fetus morphology and skeleton development of rats after feddiet containingCalliandra leaf flour. This study used a completely randomized design with 16 pregnant rats that were dividedinto four treatment groups i.e. 0, 10, 17.5 and 25% of Calliandra leaf flour. The diets were given 20 g/rat/dayeach, twice a day, for 30 days (7 days of diet adaptation, 21 days of gestation period, and one day after birth).One day after the birth, all fetuses were separated from their mother for further preparation and analysis. Thedata were analyzed statistically by using SPSS 22 version. If they were distributed normally, the data would beanalyzed usingOneway ANOVA and Duncan Multiple Range Test (DMRT) to see the difference among the groups.Diets containing Calliandra leaf flour fed on pregnant rats decreased weight and length of fetuses and increasedfetus hemorrhage. However, it did not cause dead fetus or decreased the number of life fetuses. Diets containingCalliandra leaf flourfed to pregnant rats did not affect the ossification of vertebrae, costae and sternebrae. However,it inhibited ossification of metacarpus, metatarsus and vertebrae caudalis.
SUGAR BIOSYNTHESIS IN VANILLA PLANTS AND PHALAENOPSIS SEEDLINGS AFTER APPLICATION OF FERTILIZERS
Authors: Adiputra I Gede Ketut
Published: March 2018

In tropical country, vanilla encounters two main environmental conditions, wet and dry seasons. In the wet seasons when the amount of water available favours high growth rate, plants acquire more nutrients. However during dry seasons when water uptake via the root is limited and water loss from the shoot is increasing, vanilla will experience severe desiccations. In other cases, under condition of culture in bottle, phalaenopsis seedlings develop a very thin cuticle layers. These seedlings will experience a high rate of water loss when it is then transferred into ex vitro environmental conditions. Since mineral nutrient could decrease water potential in plants and enhanced by water loss, application of proper fertilizer into these vanilla plants and phalaenopsis seedling should not enhance the decreasing of water potential which could impair physiological mechanism. So, in order to ascertain nutrient requirement for these plants, two separate experiments were conducted. In the first experiment, vanilla plants grown in plantation were added 9.25, 1.1 and 4.6 g/plant Urea, TSP and KCL respectively. This experiment was repeated using different plants with application of fertilizer in higher doses; 18.5, 2.2 and 9.2 g/plant for Urea, TSPand KCl, respectively. In order to examine the capacity of these plants to synthesize sugar, samples were then harvested in the period after fertilizer application, i.e. at day 0, 11 and 24. In the second experiments, phalaenopsis seedlings were sprayed every 1-2 days with liquinox and rosasol-N fertilizer using factorial experimental design.. In order to examine the effect of the fertilizer on sugar biosynthesis, leaves of these plants were harvested at day 132. These experiments showed that addition of fertilizer into vanilla plants increased sugar content but addition of fertilizer into phalaenopsis seedling decreased sugar content. It is concluded that fertilizer applied into plants undergoing desiccation impair sugar biosynthesis.
Motivation in Mathematics Learning
Authors: Fuqoha A A N , Budiyono Budiyono , Indriati D
Published: March 2018

The purpose of this research is to know the motivation of mathematics learning in high school students of 10th grade. Data collection method is done in the form of survey conducted in Purbalingga. The instrument used is a questionnaire of motivation to learn math. Interview method is also done to get additional data which is used as supporting data in conducting analysis of data obtained previously. The result of the survey shows that the students of both schools have a high motivation to learn math with an average of 3.39. Based on the results of the analysis, a lot of the students who have high learning motivation on math dissatisfied with the value of daily tests and examinations with an average index of 2.31. It shows that high learning motivation is not enough to get a satisfactory value. The statement was corroborated by points on the understanding of mathematical material that was only able to obtain an average index of 2.56. Based on the results of interviews from 3 students, they wanted to use a good learning media that had three characteristic, that was a learning media that taught students not to give up easily, that was able to bring out a sense of pleasure, and that was challenging. Therefore, beside high learning motivation, understanding of mathematics materials supported by the use of good learning media is also required.
Media and Metamedia Management, de Francisco Campos Freire, Xosé Rúas Araújo, Valentín Alejandro Martínez Fernández, Xosé López García (Ed.)
Authors: Robledo Dioses Kelly
Published: March 2018

Diversos procesos sociales, culturales, económicos, empresariales e, incluso, políticos, se ven envueltos y condicionados por un contexto comunicacional en constante desarrollo. La creciente evolución de los medios y el ecosistema creado en torno y a consecuencia de ellos resulta ser un gran desafío no solo en términos tecnológicos, sino en modos de contar y en nuevos públicos a los que llegar.
Rachel de queiroz: uma mulher à frente do seu tempo
Authors: De Oliveira Maria Eveuma , Freire Manoel , Freire Chaves Sérgio Wellington
Published: March 2018

ResunoA relação da mulher com a literatura e com o mundo da escrita tem sido objeto de estudo de vários historiadores. Os romances de Rachel de Queiroz se destacam por enfatizarem aspectos sociais e tratarem de valores de uma época nas várias tramas que vivem seus personagens. O artigo objetiva mostrar a relevância do trabalho da escritora como fomento da escrita feminina para a literatura brasileira.Palavras-chaveHistória. Expressão literária. Gênero.Trabalho.
PROFEMINIS DAN KONTRAFEMINIS TOKOH HANAH DALAM CERPEN TELAPAK KAKI YANG MENYIMPAN SURGA KARYA NI KOMANG ARIANI
Authors: Irmawati Siti Elly , Chamalah Evi , Turahmat Turahmat
Published: March 2018

The background of short story is about intricacies of Hanah�s household life, which shows the character of Hanah�s obidience that always be in theauthorization of man, that is her husband. This study in general aimed to obtainthe description about prosfeminism and consfeminism of Hanah's figure in theshort story Telapak Kaki yang Menyimpan Surgawhich was written by Ni Komang Ariani in the Jawa Pos newspaper whichis published on 18 September 2016. The method used in this study was descriptive qualitative methods. Furthemore the approach of feminism was done to knowing about prosfeminism and consfeminism of Hanah as a wife or women in general. The results of research showed that Hanah belonged consfeminism because she was more obeyed anything her husband's told then doing activity outside the house. But,actually Hanah wished to go to school and do activities outside the house because her husband demanded to commit polygamy.
Identifikasi Ikan Genus Mystus Dengan Pendekatan Genetik
Authors: Pramono Taufik Budhi , Arfiati Diana Arfiati , Widodo Maheno Sri , Yanuhar Uun Yanuhar
Published: March 2018

Ikan-ikan dari Famili Bagridae di Indonesia mencapai 60 jenis dan salah satunya dari genus Msytus. Metode yang digunakan untuk identifikasi ikan yang sangat cepat dan akurat sangat diperlukan. DNA barcoding adalah teknik identifikasi baru dengan pendekatan molekular. Gen CO1 diamplifikasi dan produk PCR disekuensing serta dianalisis dengan menggunakan software bioinformatika. Pengeditan hasil sekuensing dan penentuan komposisi nukleotida dianalisis dengan software Mega5. Urutan DNA disejajarkan dengan ClustalW vers. 1.4. Sekuens dibandingkan dengan data GenBank menggunakan BLAST (Basic Local Aligment Search Tools) dan BOLDSystems. Pohon filogenetik dibuat dengan menggunakan metode Neighbor_Joining. Salah satu contoh identifikasi ikan dari genus Mystus yang telah dikonfirmasi adalah Mystus nigriceps menjadi Mystus singaringan.
Effect of extracts of Allium porrum in some hematological parameters of rat
Authors: Albsaysi Azhar Shya Y.
Published: March 2018

The research was aimed to know the effect of aqueous and alcoholic extracts of Allium porrum on some hematological parameters of male rats (n=35). Rats were divided into groups and  were administrated different concentrations (200, 400, and 800) mg/kg of extract daily for one week to study the parameters of hematological parameters compared to control group. The treatment with A. porrum extracts led to increasing RBC and P.C.V. for all extract concentrations and to both kinds. The aqueous extract 800 mg/kg shows more significant effect than the other levels. A significant increase in hemoglobin concentration for aqueous extract 800 mg/kg and 400 mg/kg and alcoholic extract 800 mg/kg and alcoholic extract 800 mg/kg were more significant than the other levels and a significant decrease in WBC count for alcoholic extract 400 mg/kg compared to the control group.
Correlation of CA-125 with different stages of endometriosis
Authors: Laila Runa , Nasrin Begum , Shamim Shayela , Hoque Md. Mozammel
Published: March 2018

This study was conducted to evaluate the association of serum cancer antigen (CA-125) level with the severity of pelvic endometriosis. Seventy diagnosed cases of pelvic endometriosis were included in this study. The CA-125 level was estimated in all these patients, cutoff value of the serum CA-125 level was considered 35.0 U/mL. The correlations between serum CA-125 and different stages of endometriosis were evaluated by linear regression analysis. In Stage I of endometriosis, the mean serum CA-125 level was 21.8 ± 15.1 U/mL, in Stage II 26.0 ± 17.3 U/mL, in Stage III 83.2 ± 48.9 U/mL and in Stage IV 117.0 ± 41.6 U/mL. A significant positive correlation (r=0.729; p=0.001) was found between the serum CA-125 and different stages of endometriosis.
Facile synthesis of a ZnO–BiOI p–n nano-heterojunction with excellent visible-light photocatalytic activity
Authors: Zhang Mengyuan , Qin Jiaqian , Yu Pengfei , Zhang Bing , Ma Mingzhen , Zhang Xinyu , Liu Riping
Published: March 2018

In this paper, an efficient method to produce a ZnO/BiOI nano-heterojunction is developed by a facile solution method followed by calcination. By tuning the ratio of Zn/Bi, the morphology varies from nanoplates, flowers to nanoparticles. The heterojunction formed between ZnO and BiOI decreases the recombination rate of photogenerated carriers and enhances the photocatalytic activity of ZnO/BiOI composites. The obtained ZnO/BiOI heterostructured nanocomposites exhibit a significant improvement in the photodegradation of rhodamine B under visible light (λ ≥ 420 nm) irradiation as compared to single-phase ZnO and BiOI. A sample with a Zn/Bi ratio of 3:1 showed the highest photocatalytic activity (≈99.3% after 100 min irradiation). The photodegradation tests indicated that the ZnO/BiOI heterostructured nanocomposites not only exhibit remarkably enhanced and sustainable photocatalytic activity, but also show good recyclability. The excellent photocatalytic activity could be attributed to the high separation efficiency of the photoinduced electron–hole pairs as well as the high specific area.
Graphene composites with dental and biomedical applicability
Authors: Malik Sharali , Ruddock Felicite M , Dowling Adam H , Byrne Kevin , Schmitt Wolfgang , Khalakhan Ivan , Nemoto Yoshihiro , Guo Hongxuan , Shrestha Lok Kumar , Ariga Katsuhiko , Hill Jonathan P
Published: March 2018

Pure graphene in the form of few-layer graphene (FLG) – 1 to 6 layers – is biocompatible and non-cytotoxic. This makes FLG an ideal material to incorporate into dental polymers to increase their strength and durability. It is well known that graphene has high mechanical strength and has been shown to enhance the mechanical, physical and chemical properties of biomaterials. However, for commercial applicability, methods to produce larger than lab-scale quantities of graphene are required. Here, we present a simple method to make large quantities of FLG starting with commercially available multi-layer graphene (MLG). This FLG material was then used to fabricate graphene dental-polymer composites. The resultant graphene-modified composites show that low concentrations of graphene (ca. 0.2 wt %) lead to enhanced performance improvement in physio-mechanical properties – the mean compressive strength increased by 27% and the mean compressive modulus increased by 22%. Herein we report a new, cheap and simple method to make large quantities of few-layer graphene which was then incorporated into a common dental polymer to fabricate graphene-composites which shows very promising mechanical properties.
Instructor-Created Activities to Engage Undergraduate Nursing Research Students
Authors: Pierce Linda L. , Reuille Kristina M.
Published: March 2018

Background:

In flipped or blended classrooms, instruction intentionally shifts to a student-centered model for a problem-based learning approach, where class time explores topics in greater depth, creating meaningful learning opportunities.

Method:

This article describes instructor-created activities focused on research processes linked to evidence-based practice that engage undergraduate nursing research students. In the classroom, these activities include individual and team work to foster critical thinking and stimulate student discussion of topic material.

Results:

Six activities for small and large student groups are related to quantitative, qualitative, and both research processes, as well as applying research evidence to practice. Positive student outcomes included quantitative success on assignments and robust student topic discussions, along with instructor-noted overall group engagement and interest.

Conclusion:

Using these activities can result in class time for the construction of meaning, rather than primarily information transmission. Instructors may adopt these activities to involve and stimulate students' critical thinking about research and evidence-based practice. [
J Nurs Educ.
2018;57(3):174–177.]
New Careers in Nursing: An Effective Model for Increasing Nursing Workforce Diversity
Authors: Craft-Blacksheare Melva
Published: March 2018

Background:

The Robert Wood Johnson Foundation and the American Association of Colleges of Nursing developed the New Careers in Nursing (NCIN) program to address the nursing shortage, increase workforce diversity, and raise the profession's educational level. The program provided scholarships to second-degree underrepresented or economically disadvantaged (UED) students attending an accelerated nursing program to earn a Bachelor of Science in Nursing degree.

Method:

A midwestern university received three academic-year cycles of NCIN funding. The program's model, resources, and functioning are described.

Results:

The NCIN provided exceptional financial and program support that received high marks from participants. During the three award cycles, 20 UED scholars graduated with a Bachelor of Science in Nursing degree. Nineteen of the 20 scholars passed the NCLEX-RN on the first attempt.

Conclusion:

While the NCIN program has ended, nursing school administrators and faculty wishing to promote UED student success should consider using the program's model and resources as the basis for their own program. [
J Nurs Educ.
2018;57(3):178–183.]
Obsessive-Compulsive Disorder in Children and Adolescents
Authors: Tobin Valerie
Published: March 2018

Obsessive-compulsive disorder (OCD) can be a disabling condition for children, adolescents, and their families. The majority of children and adolescents with OCD have poor academic functioning and a comorbid diagnosis. Many patients will respond with exposure and response prevention therapy. However, for those who are unable, unwilling, or do not fully respond to conservative treatment, it is essential that advanced practice nurses are able to appropriately evaluate and prescribe for OCD in their young patients. [
Journal of Psychosocial Nursing and Mental Health Services, 56
(3), 15–18.]
Influencing Factors of University Students’ Use of Social Network Sites: An Empirical Analysis in China
Authors: Liu Liqiong , Zhang Liyi , Ye Pinghao , Liu Qihua
Published: March 2018

This paper explores the influencing factors of Chinese university students in accepting and using social networking sites (SNS) to propose measures and recommendations that can guide and help these students correctly use SNS. In addition, this paper aims to provide theoretical support in increasing user loyalty for the SNS service providers and attract new users. The correlation and multiple regression analyses showed that perceived value, enjoyment, and influence positively influence the intention of individuals to use SNS.
Influence Factors of Satisfaction with Mobile Learning APP: An Empirical Analysis of China
Authors: Liu Liqiong , Zhang Liyi , Ye Pinghao , Liu Qihua
Published: March 2018

The main purpose of this study is to construct the user satisfaction structure model of mobile learning APP (software designed that run on mobile devices) from the perspective of the mobile learning participants, and to analyze the influence factors of satisfaction with mobile learning APP. The results of this study show that perceived responsiveness and perceived content are both the important factors that affect user satisfaction with mobile learning APP. Users have high satisfaction with the content conforming to the mobile learning context. Users expect to get immediate feedback in the process of learning, which can improve the learning methods and efficiency.
A Study on the Application of Automatic Scoring and Feedback System in College English Writing
Authors: Lv Xiaoxiao
Published: March 2018

With the rapid development of computer technology and the need of college Eng-lish teaching, the automatic grading of college students' English writing level is an inevitable trend. This is the inevitable result of the combination of computer tech-nology and English teaching. The theoretical basis of this paper is the feedback of English writing and related theories introduced as follows. This paper analyzes the influence of automatic scoring and feedback system on English writing level via testing the variation of student composition’s grammar, structure, length and grade in a teaching experiment. The experimental results indicate that online feed-back is effective in evaluating students' English writing proficiency, which can help students improve their writing proficiency and independent writing ability. The students' attitudes and views towards automatic grading and feedback system were investigated with 118 students in the English experiment class of SUST as the test object. The research results can suggest a guide line in the improvement of college students’ writing competence and evaluation of the effect of automatic scoring feedback system.
Application of Browser/Server Architecture in College English Online Learning System Design
Authors: Mao Lidan
Published: March 2018

In order to realize the information management of college English learning, im-prove the management efficiency of college English teaching, and reform the tra-ditional applied teaching mode, this paper proposed to design a college English online learning system, which uses Browser/Server (B/S) as the architecture, JSP technology as the programing language, and MySQL database technology as the data storage and management database. Then, this paper fully analyzed the re-quirements of network English teaching and management and the demands of system users. On this basis, functional module, database design and overall sys-tem architecture were designed, with the relevant interface displayed. The design and implementation of the system can improve the efficiency and quality of Eng-lish teaching and effectively advance the teaching level of college English, which can be applied into extensive fields.
Design and Implementation of English for Academic Purpose Online Learning System Based on Browser/Server Framework
Authors: Gong Yan
Published: March 2018

Today, with the rapid development of the information age, the education reform tends to be internationalized. The tertiary-level EFL education in colleges and universities has also changed its original model with focuses on cultivating gen-eral-purpose linguistic skills to one on students' English for Academic Purpose (EAP). EAP English instruction has been vigorously popularized in research-based universities. To achieve the informationized and standardized management for EAP English instruction work in the universities, in this paper, we design and develop a EAP English online learning system with B / S as the system develop-ment framework by which the system's overall functions are designed. MySQL is chosen as a database development tool used to implement the main object mod-ule, while JSP technology is used to support the cross-platform mechanism in order to access to diversified data sources. It is proved by the test on system op-eration that this system features operability, easy to use and maintain, and enables to meet the needs of university students for EAP English learning and teaching management, improves the students’ EAP English learning model and efficiency.
The Educational Affordances of Mobile Instant Messaging (MIM)
Authors: Klein Amarolinda Zanela , Junior José Carlos da Silva Freitas , Mattiello da Silva Juliana Vitória Vieira Mattiello , Barbosa Jorge Luis Victória , Baldasso Lucas
Published: March 2018

The popularity of Mobile Instant Messaging (MIM) has prompted educators to integrate it in teaching and learning in higher education. WhatsApp® is a multi-platform instant messaging application widely used worldwide, however, there is still little applied research on its use as a platform for educational activities in management higher education. In this article, the authors present a quantitative and qualitative assessment of a concrete experience of WhatsApp® use that involved 140 undergraduate management students. Data were collected through questionnaires answered by the participants after the end of the experience of use, and also via content analysis of their conversations inside their WhatsApp® groups. The results indicate five main educational affordances of MIM that can be considered in management education: interactivity, knowledge sharing, sense of presence, collaboration and ubiquity. The article also explores the limitations of this tool and provides suggestions of good practices of MIM use for teaching and learning.
The Role of Reputation on Trust and Loyalty
Authors: Sunday Adewale Olaleye  , Salo Jari , Ukpabi Dandison C.
Published: March 2018

The purpose of this article is to empirically examine the role of online retailer's website reputation on tablet commerce and to compare the trust arbitration between reputation and loyalty in two cultures - Finland and Nigeria. Data was collected from Finland and Nigeria, using purposive and snowball sampling methods. This article uses Structural Equation Modelling and mediation analysis. The results reveal that the reputation of online retailer's website is not a direct determinant of loyalty for online shopping while trust colligates with ease of use. The effect of an online retailer's website reputation is stronger in Nigeria than in Finland and trust is a strong predictor of ease of use in Finland than Nigeria. Furthermore, trust fully mediates the effect of reputation on loyalty in Finland and partially mediates the effect of reputation and loyalty in Nigeria. The implication of the result on existing and future managers were discussed with future direction.
Player-Driven Video Analysis to Enhance Reflective Soccer Practice in Talent Development
Authors: Hjort Anders , Henriksen Kristoffer , Elbæk Lars
Published: March 2018

In the present article, we investigate the introduction of a cloud-based video analysis platform called Player Universe (PU). Video analysis is not a new performance-enhancing element in sports, but PU is innovative in how it facilitates reflective learning. Video analysis is executed in the PU platform by involving the players in the analysis process, in the sense that they are encouraged to tag game actions in video-documented soccer matches. Following this, players can get virtual feedback from their coach. Findings show that PU can improve youth soccer players' reflection skills through consistent video analyses and tagging; coaches are important as role models and providers of feedback; and that the use of the platform primarily stimulated deliberate practice activities. PU can be seen as a source of inspiration for soccer players and clubs as to how analytical platforms can motivate and enhance reflective learning for better in-game performance.
Let's Move!
Authors: Finco Mateus David , Rocha Richard Santin , Fão Rafael Wailla , Santos Fabiana
Published: March 2018

The aim of this article was to analyze how players of Pokémon GO could adopt a healthier and active lifestyle meanwhile or after using the game, observing how active they could become in their daily routines. The methodology involved a qualitative analysis involving a sample with players who were invited to complete an online questionnaire to answer specific questions about lifestyle and healthy choices. This study involved 125 players (84 males and 41 females) in the city of Pelotas (South of Brazil) that have played for at least six months the game. As results, it was possible to observe that users have changed many habits, specifically regarding physical activity gains, as going more often to practice different sports than only running, walking or cycling, and many of players were getting into an active living practicing exercises with friends and family, out of the game. Also, many players commented that meeting new users was a good way to socialize and making groups to walk or run together, getting an extra motivation for other activities out of the game. We conclude that Pokémon GO is one of the first mobile-based gameplays that can be used to promote a healthier lifestyle with a new way of interaction, changing sedentary lifestyles with a big potential to be used in Health Education.
Teaching Social Studies With Games
Authors: Jančič Polona , Hus Vlasta
Published: March 2018

Social studies is a class students encounter in the fourth and fifth grades of primary school in Slovenia. It includes goals from the fields of geography, sociology, history, ethnology, psychology, economy, politics, ethics, aesthetics, and ecology. Among other didactic recommendations in the national curriculum for teaching, social studies include experiential learning with games. Game-based learning enables an optimal learning environment for students. The purpose of this article is to examine representation of games in social studies in primary school. The research sample consisted of 290 students of the fourth and fifth grade, 177 teachers teaching fourth and fifth grade, and 56 observed social studies lessons. Results showed that teachers rarely use didactic games in social studies. Results show that teachers rarely use game-based learning in teaching social science. Depending on the type of a game, the most commonly used one is a role-playing game. Most respondents' students like game-based learning in social studies and also estimate games are not played often enough in social studies.
The Affordances of Mobile-App Supported Teacher Observations for Peer Feedback
Authors: Çelik Sercan , Baran Evrim , Sert Olcay
Published: March 2018

Mobile technologies offer new affordances for teacher observation in teacher education programs, albeit under-examined in contrast to video technologies. The purpose of this article is to investigate the integration of mobile technologies into teacher observation. Using a case study method, the authors compare the traditional narrative paper-pen, mobile app-supported, and video observation methods. Participants included 2 experienced teachers of English as a Foreign Language who were selected as the observers and observees in a higher education institutional context. The data was collected in three different teaching sessions over 4 weeks. Data sources included lesson observation notes and semi structured interviews conducted with teachers after each session. Results suggest recommendations for the integration of mobile and video based observation tools into teacher professional development programs, pre-service and in-service teacher education programs, as well as teacher certificate programs.
Designing an Educator Toolkit for the Mobile Learning Age
Authors: Burden Kevin , Kearney Matthew
Published: March 2018

Mobile technologies have been described as ‘boundary' objects which enable teachers and learners to transcend many of the barriers such as rigid schedules and spaces which have hitherto characterised traditional forms of education. However, educators need to better understand how to design learning scenarios which genuinely exploit the unique pedagogical affordances of mobile technologies rather than replicating existing patterns and modes of behaviour. This article describes the design and development of a mobile learning toolkit for educators to realise this vision. It presents the theoretical underpinnings for the toolkit and describes the development of different tools, instruments and resources. The main aim of the toolkit is to help teacher educators and teachers build knowledge and understanding of more diverse mobile pedagogical approaches.