bluuebunny/crossref_metadata_2025 huggingface dataset:
A novel phenolic derivative inhibits AHL-dependent quorum sensing signaling in Pseudomonas aeruginosa
Authors: Bernabè Giulia , Marzaro Giovanni , Di Pietra Giuseppe , Otero Ana , Bellato Massimo , Pauletto Anthony , Scarpa Melania , Sut Stefania , Chilin Adriana , Dall’Acqua Stefano , Brun Paola , Castagliuolo Ignazio
Published: September 2022

Increasing antibiotic resistance and the decline in the pharmaceutical industry’s investments have amplified the need for novel treatments for multidrug-resistant bacteria. Quorum sensing (QS) inhibitors reduce pathogens’ virulence without selective pressure on bacteria and provide an alternative to conventional antibiotic-based therapies. P. aeruginosa uses complex QS signaling to control virulence and biofilm formation. We aimed to identify inhibitors of P. aeruginosa QS acting on acyl-homoserine lactones (AHL)-mediated circuits. Bioluminescence and qRT-PCR assays were employed to screen a library of 81 small phenolic derivatives to reduce AHL-dependent signaling. We identified GM-50 as the most active compound inhibiting the expression of AHL-regulated genes but devoid of cytotoxic activity in human epithelial cells and biocidal effects on bacteria. GM-50 reduces virulence factors such as rhamnolipids, pyocyanin, elastase secretion, and swarming motility in P. aeruginosa PAO1 laboratory strain. By molecular docking, we provide evidence that GM-50 highly interacts with RhlR. GM-50 significantly improved aztreonam-mediated biofilm disruption. Moreover, GM-50 prevents adhesion of PAO1 and inflammatory damage in the human A549 cell line and protects Galleria mellonella from PAO1-mediated killing. GM-50 significantly reduces virulence factors in 20 P. aeruginosa clinical isolates from patients with respiratory tract infections. In conclusion, GM-50 inhibits AHL-signaling, reduces virulence factors, enhances the anti-biofilm activity of aztreonam, and protects G. mellonella larvae from damage induced by P. aeruginosa. Since GM-50 is active on clinical strains, it represents a starting point for identifying and developing new phenolic derivatives acting as QS-inhibitors in P. aeruginosa infections.
Network pharmacology and experimental verification-based strategy to explore the underlying mechanism of Liu Jun An Wei formula in the treatment of gastrointestinal reactions caused by chemotherapy for colorectal cancer
Authors: Li Gaobiao , Liu Liying , Yin Yiran , Wang Mengmeng , Wang Lei , Dou Jianwei , Wu Hongwei , Yang Yufei , He Bin
Published: September 2022

Background: Liu Jun An Wei formula (LJAW), derived from “Liu Jun Zi Decoction”, is a classical prescription of Tradition Chinese Medicine and has been used for the treatment of gastrointestinal reactions caused by chemotherapy for colorectal cancer (CRC) for many years. Its molecular mechanism remains to be further explored.

Objective: To clarify the mechanism of LJAW in attenuating gastrointestinal reactions caused by chemotherapy for CRC.

Methods: The 5-fluorouracil (5-FU) induced mouse and intestine organoid models were established to observe the effect of LJAW. The ingredients of LJAW were analyzed and identified by UPLC-Q-TOF-MS technology. Targets of LJAW and chemotherapy-induced gastrointestinal reactions were collected from several databases. “Ingredient-target” network and protein-protein interaction network were constructed based on network pharmacology. Then, gene ontology (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed. Subsequently, molecular docking method was used to verify the interaction between the core ingredients and key targets. The results were validated by both in vivo experiments and organoid experiments. Western Blot was used to analyze the influence of LJAW on key targets including PI3K, AKT1, MAPK1, MAPK14 proteins and their phosphorylated proteins. RT-qPCR and Western Blot were used to detect the mRNA and protein levels of apoptosis-related gene PUMA.

Results: Compared with the 5-FU group, the LJAW group had better morphology in mouse small intestine and intestine organoids. In total, 18 core ingredients and 19 key targets were obtained from 97 ingredients and 169 common targets. KEGG analysis showed that the common targets were involved in PI3K/Akt, MAPK, apoptosis and other signal pathways, which are closely related to gastrointestinal injury. Experiments confirmed that LJAW lowered the expressions of phosphorylated proteins including p-PI3K, p-AKT1, p-MAPK1, and p-MAPK14 and reduced the mRNA and protein levels of PUMA.

Conclusion: LJAW shows protective effect on 5-FU induced small intestine and intestinal organoids injury. LJAW attenuates gastrointestinal reactions caused by chemotherapy for CRC probably by regulating apoptosis-related genes through PI3K/AKT and MAPK signaling pathways.
Salvia miltiorrhiza in thorax and abdomainal organ fibrosis: A review of its pharmacology
Authors: Yang Zhao , Qi Jingshu , Ping Dabing , Sun Xin , Tao Yanyan , Liu Chenghai , Peng Yuan
Published: September 2022

Organ fibrosis is a common pathological change that finally results in organ failure, which involves the destruction of parenchyma cells, the activation of mesenchymal cells and the imbalance of immunological cells. In recent years, although some breakthroughs have been made in understanding the pathogenesis and therapeutics of organ fibrosis, no registered drugs could directly target the fibrotic process, which constitutes a major biomedical challenge. Salvia miltiorrhiza (SM) is a well-known medicinal plant in China, which has been widely applied because of its pharmacological effects on anti-oxidative, anti-myocardial infarction, anti-fibrotic, anti-inflammatory, and anti-neoplastic properties. Accumulated evidence suggested that SM played critical roles against organ fibrosis in vivo and in vitro experiments by its multiple biological compounds. In this review, we discussed the recent advances on the phytochemistry and pharmacological mechanisms of SM and its active ingredients in liver, lung, kidney, and heart fibrosis, which might help to promote the treatment of fibrotic diseases in thorax and abdomainal viscera in clinic.
Portable flexible probe for detecting blood supply status in clinical surgery
Authors: Xia Jie , Pan Jiadong , Yan Ruijian , Zhang Fan , Zhang Luxi , Feng Gang , Luo Jack , Dong Shurong , Wang Yueming
Published: September 2022

In the medical field, it is important to monitor and evaluate the blood supply status of organs and tissues during the clinical surgery. However, this largely depends on the surgeon’s experience and naked eye, which is easy to misjudge due to the interference of blood stains or other factors. A portable and flexible photoplethysmographic (PPG) detection probe is developed in this paper. And a new evaluation methodology of blood supply status is proposed based on this probe. Three typical indicators based on PPG is proposed to comprehensively evaluate the blood supply status, which are the blood oxygen saturation and its pulsation, differential characteristics of different lights, and time-frequency energy spectral characteristic. The probe and its evaluation methodology are verified using the brain of rats as a model.
Changes in aortic diameter induced by weight loss: The HELENA trial- whole-body MR imaging in a dietary intervention trial
Authors: Stoll Sibylle , Sowah Solomon A. , Fink Matthias A. , Nonnenmacher Tobias , Graf Mirja E. , Johnson Theron , Schlett Christopher L. , von Stackelberg Oyunbileg , Kirsten Romy , Bamberg Fabian , Keller Jeffrey , Ulrich Cornelia M. , Kaaks Rudolf , Kauczor Hans-Ulrich , Rengier Fabian , Kühn Tilman , Nattenmüller Johanna
Published: September 2022

Obesity-related metabolic disorders such as hypertension, hyperlipidemia and chronic inflammation have been associated with aortic dilatation and resulting in aortic aneurysms in many cases. Whether weight loss may reduce the risk of aortic dilatation is not clear. In this study, the diameter of the descending thoracic aorta, infrarenal abdominal aorta and aortic bifurcation of 144 overweight or obese non-smoking adults were measured by MR-imaging, at baseline, and 12 and 50 weeks after weight loss by calorie restriction. Changes in aortic diameter, anthropometric measures and body composition and metabolic markers were evaluated using linear mixed models. The association of the aortic diameters with the aforementioned clinical parameters was analyzed using Spearman`s correlation. Weight loss was associated with a reduction in the thoracic and abdominal aortic diameters 12 weeks after weight loss (predicted relative differences for Quartile 4: 2.5% ± 0.5 and -2.2% ± 0.8, p < 0.031; respectively). Furthermore, there was a nominal reduction in aortic diameters during the 50-weeks follow-up period. Aortic diameters were positively associated with weight, visceral adipose tissue, glucose, HbA1c and with both systolic and diastolic blood pressure. Weight loss induced by calorie restriction may reduce aortic diameters. Future studies are needed to investigate, whether the reduction of aortic diameters via calorie restriction may help to prevent aortic aneurysms.
Changes of Neolithic subsistence in south Hangzhou Bay coast, eastern China: An adaptive strategy to landscape processes
Authors: Dai Jinqi , Deng Lanjie , Feng Dan , Zhao Xiaoshuang , Wang Huimin , Li Xueming , Xiao Li , Zhang Xiaoyu , Chen Jing , Li Maotian , Chen Zhongyuan , Liu Yan , Sun Qianli
Published: September 2022

The transition from hunting and gathering to agricultural subsistence is a striking feature of the Neolithic revolution worldwide. Known as the cradle of a series of representative Neolithic cultures, south Hangzhou Bay (SHB) witnessed substantial changes in both landscape and human subsistence during the Holocene, yet the relationship between them was not well established. Here, we combined archaeobotanical results from sediment cores with archaeological findings to illustrate the subsistence changes during the Neolithic regime in the context of the landscape process in SHB. Our result showed that SHB was inundated by marine transgression 8,200 years ago without significant human imprints. At 8,200–7,600 cal yr. BP, the initial coastal wetland formation at locations with the semi-enclosed landscape would have facilitated the activities of hunting-gathering, incipient rice cultivation, and collecting seafood if accessible. Pollen and phytoliths evidence from multiple sediment cores in the Yaojiang Valley (YJV) suggested a desalinization process of wetland in the following hundreds of years. This amelioration of the environment had favored the intermittent rice cultivation at various locations in the YJV, where archaeological evidence was absent. Since 7,000–6,600 cal yr. BP, as freshwater wetland expanded with coastal progradation, a wide variety of food resources became available. Meanwhile, rice domestication began to serve as a crucial food supplement as evidenced by both microfossil results and archaeological findings. With the expansion of the coastal plain after 5,500 cal yr. BP, rice farming became widespread and rice consumption was increasingly important in the diet, as supported by discoveries of upgraded farming tools, abundant rice remains, and ancient rice paddies. Above all, the change of subsistence from hunting-gathering to rice farming exhibited an adaptive strategy in response to landscape evolution from an initial marine-influenced setting to a later coastal plain.
Advances in molecular mechanisms underlying cadmium uptake and translocation in rice
Authors: Ai Hao , Wu Daxia , Li Chunli , Hou Mengmeng
Published: September 2022

The increasing cadmium (Cd) pollution in paddy fields has severely threatened China’s ecological and food safety. Cultivation of low Cd accumulation varieties to reduce Cd content in rice or cultivation of Cd-tolerant varieties for phytoremediation are considered effective methods to control Cd pollution in paddy fields. However, the underlying molecular mechanism of Cd absorption and transport by rice plants needs to be deciphered to cultivate these varieties. Here, we summarized the molecular mechanisms underlying Cd absorption and transport in rice, as well as the variation of Cd accumulation among rice varieties, the QTLs related to Cd accumulation in rice, and discusses the direction of future research.
Fine mapping of the Hairy glume (Hg) gene in a chromosome variation region at the distal terminus of 1AS
Authors: Luo Wei , Zhou Jieguang , Liu Jiajun , Liu Yanlin , Mu Yang , Tang Huaping , Xu Qiang , Deng Mei , Jiang Qiantao , Chen Guoyue , Qi Pengfei , Wang Jirui , Jiang Yunfeng , Chen Zhongxu , Zheng Zhi , Wei Yuming , Zheng Youliang , Lan Xiujin , Ma Jian
Published: September 2022

Trichomes are differentiated epidermal cells and exist on above-ground organs of nearly all land plants with important roles in resistance to a wide range of biotic and abiotic stresses. We attempted to obtain candidate gene (s) for Hairy glume (Hg), responsible for the trichome on wheat glume, by using bulked segregant exome capture sequencing (BSE-Seq), while Hg was only mapped in 0.52–3.26 Mb of 1AS. To further fine map this gene and identify candidate genes in this region, a near isogenic line-derived population consisting of 2,050 F2 lines was generated in the present study. By analyzing this population, Hg was fine mapped into a 0.90 cM region covering a physical distance of ~825.03 Kb encompassing 6 high- and 23 low-confidence genes in the reference genome of Chinese Spring. A presence-absence variation was identified in the fine mapping region through analyses of sequence-tagged sites markers and genome sequences of the hairy glume parent of the near isogenic lines. The results presented here will be useful for further cloning Hg in wheat.
A soybean sodium/hydrogen exchanger GmNHX6 confers plant alkaline salt tolerance by regulating Na+/K+ homeostasis
Authors: Jin Ting , An Jiaxin , Xu Huadong , Chen Jie , Pan Lang , Zhao Ranran , Wang Ning , Gai Junyi , Li Yan
Published: September 2022

Alkaline soil has a high pH due to carbonate salts and usually causes more detrimental effects on crop growth than saline soil. Sodium hydrogen exchangers (NHXs) are pivotal regulators of cellular Na+/K+ and pH homeostasis, which is essential for salt tolerance; however, their role in alkaline salt tolerance is largely unknown. Therefore, in this study, we investigated the function of a soybean NHX gene, GmNHX6, in plant response to alkaline salt stress. GmNHX6 encodes a Golgi-localized sodium/hydrogen exchanger, and its transcript abundance is more upregulated in alkaline salt tolerant soybean variety in response to NaHCO3 stress. Ectopic expression of GmNHX6 in Arabidopsis enhanced alkaline salt tolerance by maintaining high K+ content and low Na+/K+ ratio. Overexpression of GmNHX6 also improved soybean tolerance to alkaline salt stress. A single nucleotide polymorphism in the promoter region of NHX6 is associated with the alkaline salt tolerance in soybean germplasm. A superior promoter of GmNHX6 was isolated from an alkaline salt tolerant soybean variety, which showed stronger activity than the promoter from an alkaline salt sensitive soybean variety in response to alkali stress, by luciferase transient expression assays. Our results suggested soybean NHX6 gene plays an important role in plant tolerance to alkaline salt stress.
Genome-wide identification of calcineurin B-like protein-interacting protein kinase gene family reveals members participating in abiotic stress in the ornamental woody plant Lagerstroemia indica
Authors: Yu Chunmei , Ke Yongchao , Qin Jin , Huang Yunpeng , Zhao Yanchun , Liu Yu , Wei Hui , Liu Guoyuan , Lian Bolin , Chen Yanhong , Zhong Fei , Zhang Jian
Published: September 2022

Calcineurin B-like protein-interacting protein kinases (CIPKs) play important roles in plant responses to stress. However, their function in the ornamental woody plant Lagerstroemia indica is remains unclear. In this study, the LiCIPK gene family was analyzed at the whole genome level. A total of 37 LiCIPKs, distributed across 17 chromosomes, were identified. Conserved motif analysis indicated that all LiCIPKs possess a protein kinase motif (S_TKc) and C-terminal regulatory motif (NAF), while seven LiCIPKs lack a protein phosphatase interaction (PPI) motif. 3D structure analysis further revealed that the N-terminal and C-terminal 3D-structure of 27 members are situated near to each other, while 4 members have a looser structure, and 6 members lack intact structures. The intra- and interspecies collinearity analysis, synonymous substitution rate (Ks) peaks of duplicated LiCIPKs, revealed that ∼80% of LiCIPKs were retained by the two whole genome duplication (WGD) events that occurred approximately 56.12–61.16 million year ago (MYA) and 16.24–26.34 MYA ago. The promoter of each LiCIPK contains a number of auxin, abscisic acid, gibberellic acid, salicylic acid, and drought, anaerobic, defense, stress, and wound responsive cis-elements. Of the 21 members that were successfully amplified by qPCR, 18 LiCIPKs exhibited different expression patterns under NaCl, mannitol, PEG8000, and ABA treatments. Given that LiCIPK30, the AtSOS2 ortholog, responded to all four types of stress it was selected for functional verification. LiCIPK30 complements the atsos2 phenotype in vivo. 35S:LiCIPK-overexpressing lines exhibit increased leaf area increment, chlorophyll a and b content, reactive oxygen species scavenging enzyme activity, and expression of ABF3 and RD22, while the degree of membrane lipid oxidation decreases under NaCl treatment compared to WT. The evolutionary history, and potential mechanism by which LiCIPK30 may regulate plant tolerance to salt stress were also discussed. In summary, we identified LiCIPK members involved in abiotic stress and found that LiCIPK30 transgenic Arabidopsis exhibits more salt and osmotic stress tolerance than WT. This research provides a theoretical foundation for further investigation into the function of LiCIPKs, and for mining gene resources to facilitate the cultivation and breeding of new L. indica varieties in coastal saline-alkali soil.
1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes
Authors: Li Rui , Ma Jiheng , Gu Hui , Jia Wenjun , Shao Yuanzhi , Li Wen
Published: September 2022

Ethylene burst is an important sign of the initiation of postharvest mango ripening and softening is a typical characteristic of fruit ripening. However, the intrinsic link between ethylene release and fruit softening during ripening of postharvest mangoes is still not clear. The aim of this study was to investigate the effects of ethylene and its action inhibitor 1-methylcyclopropene (1-MCP) on fruit softening and ripening and the underlying regulatory mechanisms. Results showed that ethephon (ETH) promoted ethylene release and enhanced MDA content and activities of cell wall degrading enzymes, whereas 1-MCP treatment exhibited an opposite effect. Moreover, real-time quantitative polymerase chain reaction indicated that the transcription levels of genes involved in cell wall degradation (MiPG, Miβ-GAL and MiPE), ethylene biosynthesis (MiACO1 and MiACS6) and ethylene response factor (MiERF8) were remarkably induced by ETH. Correlation analysis further revealed that the production of ethylene was significantly negatively correlated with firmness, but positively correlated with MDA content, activities of cell wall degrading enzymes and expressions of MiPG and Miβ-GAL. Furthermore, yeast one hybrid (Y1H) assay showed that MiERF2 and MiERF8 could directly bind to the promotor of MiPG and then regulate its transcription. These findings suggest that ethylene production is closely associated with fruit softening, and MiERF2 and MiERF8 and MiPG may play crucial roles in regulation of ripening and softening of postharvest mangoes.
RNAseq, transcriptome analysis and identification of DEGs involved in development and ripening of Fragaria chiloensis fruit
Authors: Gaete-Eastman Carlos , Stappung Yazmina , Molinett Sebastián , Urbina Daniela , Moya-Leon María Alejandra , Herrera Raúl
Published: September 2022

Fragaria chiloensis (Chilean strawberry) is a native species that produces fruit with an exotic pinkish color and a fruity aroma. It has a non-climacteric pattern of fruit ripening, and it is the mother of the commercial Fragaria x ananassa. The ripening of F. chiloensis fruit seems stimulated by ABA, and a complete set of genes participate in its softening, color, and aroma development. In addition, a set of transcription factors regulate the entire process, but few of them have been described. Over the last two decades, RNA-seq was used to identify genes at three fruit development/ripening stages, named C2 (unripe, large green) to C4 (full ripe), in whole fruit and fruit without achenes. A total of 204,754 contigs were assembled considering all samples, obtaining an N50 of 1.125 bp. Differentially expressed genes (DEGs) between two samples were identified, obtaining a total of 77,181 DEGs. Transcripts for genes involved in ABA biosynthesis present high and differential expression during the C2, C3, and C4 stages. Besides, contigs corresponding to ABA receptors, which interact with a regulatory network, are also differentially expressed. Genes associated with cell wall remodeling and those involved in flavonoid synthesis were also differentially expressed. An interaction network was built considering differentially expressed genes for the phenylpropanoid and flavonoid molecular pathways and having FcMYB1 as a transcription factor regulator. Identifying key genes could give an option to control the ripening of this non-climacteric fruit.
Production and secretion of functional SARS-CoV-2 spike protein in Chlamydomonas reinhardtii
Authors: Kiefer Anna Maria , Niemeyer Justus , Probst Anna , Erkel Gerhard , Schroda Michael
Published: September 2022

The spike protein is the major protein on the surface of coronaviruses. It is therefore the prominent target of neutralizing antibodies and consequently the antigen of all currently admitted vaccines against SARS-CoV-2. Since it is a 1,273-amino acids glycoprotein with 22 N-linked glycans, the production of functional, full-length spike protein was limited to higher eukaryotes. Here we report the production of full-length SARS-CoV-2 spike protein – lacking the C-terminal membrane anchor – as a secreted protein in the prefusion-stabilized conformation in the unicellular green alga Chlamydomonas reinhardtii. We show that the spike protein is efficiently cleaved at the furin cleavage site during synthesis in the alga and that cleavage is abolished upon mutation of the multi-basic cleavage site. We could enrich the spike protein from culture medium by ammonium sulfate precipitation and demonstrate its functionality based on its interaction with recombinant ACE2 and ACE2 expressed on human 293T cells. Chlamydomonas reinhardtii is a GRAS organism that can be cultivated at low cost in simple media at a large scale, making it an attractive production platform for recombinant spike protein and other biopharmaceuticals in low-income countries.
Genome-wide identification of the AlkB homologs gene family, PagALKBH9B and PagALKBH10B regulated salt stress response in Populus
Authors: Zhao Ye , Guo Qi , Cao Sen , Tian Yanting , Han Kunjin , Sun Yuhan , Li Juan , Yang Qingshan , Ji Qingju , Sederoff Ronald , Li Yun
Published: September 2022

The AlkB homologs (ALKBH) gene family regulates N6-methyladenosine (m6A) RNA methylation and is involved in plant growth and the abiotic stress response. Poplar is an important model plant for studying perennial woody plants. Poplars typically have a long juvenile period of 7–10 years, requiring long periods of time for studies of flowering or mature wood properties. Consequently, functional studies of the ALKBH genes in Populus species have been limited. Based on AtALKBHs sequence similarity with Arabidopsis thaliana, 23 PagALKBHs were identified in the genome of the poplar 84K hybrid genotype (P. alba  ×  P. tremula var. glandulosa), and gene structures and conserved domains were confirmed between homologs. The PagALKBH proteins were classified into six groups based on conserved sequence compared with human, Arabidopsis, maize, rice, wheat, tomato, barley, and grape. All homologs of PagALKBHs were tissue-specific; most were highly expressed in leaves. ALKBH9B and ALKBH10B are m6A demethylases and overexpression of their homologs PagALKBH9B and PagALKBH10B reduced m6A RNA methylation in transgenic lines. The number of adventitious roots and the biomass accumulation of transgenic lines decreased compared with WT. Therefore, PagALKBH9B and PagALKBH10B mediate m6A RNA demethylation and play a regulatory role in poplar growth and development. Overexpression of PagALKBH9B and PagALKBH10B can reduce the accumulation of H2O2 and oxidative damage by increasing the activities of SOD, POD, and CAT, and enhancing protection for Chl a/b, thereby increasing the salt tolerance of transgenic lines. However, overexpression lines were more sensitive to drought stress due to reduced proline content. This research revealed comprehensive information about the PagALKBH gene family and their roles in growth and development and responsing to salt stress of poplar.
Metabolome and transcriptome association analysis revealed key factors involved in melatonin mediated cadmium-stress tolerance in cotton
Authors: Li Ling , Yan Xuyu , Li Juan , Wu Xiang , Wang Xiukang
Published: September 2022

Cadmium (Cd), a non-essential element for plant, is a ubiquitous and highly toxic heavy metal, seriously endangering agricultural production and human health. As a nonedible economic crop, cotton (Gossypium hirsutum L.) has great potential in remediation of Cd contaminated soil, but its underlying mechanism is still unknown. Melatonin (MT), as a plant growth regulator, is involved in alleviating Cd toxicity in some plants, but the molecular mechanisms of MT-mediated Cd detoxification in cotton are largely unknown. This study investigated the possible molecular mechanisms of the MT-mediated Cd detoxification in cotton seedlings by comparative transcriptomic and metabolomic analyses. The results showed that the cotton seedlings were dwarfed and the leaves were wilted and yellow under Cd stress. The application of 50 µmol L−1 MT significantly increased the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content under Cd stress, but 100 µmol L−1 MT significantly decreased SOD activity, while increased ascorbate peroxidase (APX) activity significantly. The addition of 100 μmol L−1 MT significantly increased Cd concentration in the shoots and roots under Cd stress. RNA-seq analysis showed that 5573, 7105, 7253, 25, 198, 9 up-regulated and 6644, 7192, 7404, 9, 59, 0 down-regulated differentially expressed genes (DEGs) were identified in the comparisons of CK vs T1, CK vs T2, CK vs T3, T1 vs T2, T1 vs T3 and T2 vs T3, respectively. It was revealed that MT promoted the expression of certain related genes under Cd stress, and the effect of 100 µmol L−1 MT was better. Moreover, UPLC-MS/MS widely targeted metabolites analyses showed that 195, 150, 150, 12, 24, 59 up-regulated and 16, 11, 23, 38, 127, 66 down-regulated differentially accumulated metabolites (DAMs) were changed in the CK vs T1, CK vs T2, CK vs T3, T1 vs T2, T1 vs T3 and T2 vs T3, respectively. It was revealed that MT induced the synthesis of alkaloids and flavonoids, and inhibited or reduced the synthesis of lipids, amino acids and their derivatives. The comprehensive analyses of transcriptomic and metabolic data showed that 33 DEGs and 4 DAMs, 46 DEGs and 16 DAMs, and 1 DEGs and 1 DAMs were dominantly involved in the pathways of valine, leucine and isoleucine degradation, ABC transporter, alpha-linolenic acid metabolism, respectively. It was revealed that there were three major mechanisms involved in MT-mediated Cd detoxification in cotton, including the enhancement of antioxidant capacity regulated by APX, flavonoids and alkaloids; accumulation of secondary metabolites related to Cd chelation, such as amino acids and derivatives; and regulation of cadmium ion transportation, such as ABC transporter activation. In conclusion, this study provides new insights into the MT-mediated Cd stress response.
Influence of psychological autonomy support of peer instruction: A novel interactive approach using Instagram in language learning
Authors: Alfadda Hind , Afzaal Muhammad , Mahdi Hassan , Alaudan Rasha , Curle Samantha
Published: September 2022

This study makes an original contribution to knowledge by investigating the impact of Just-in-Time (JiT) teaching and peer instruction (PI) strategies on the promotion of students’ achievement, interaction, and motivation in English language learning. Students were recruited from an undergraduate TESOL program in the first semester of 2019 at a University in Saudi Arabia. A multiple method research design was used to address the research questions robustly and rigorously. First, a two-group quasi-experimental design was implemented. In the first group, a lecture-based strategy was used (n = 28), while in the second group, JiT and PI strategies were used (n = 27) in English language lessons. An innovative research method was used in this study: a private Instagram account was created, serving as a platform through which teacher and students could communicate using interactive posts and comments. Second, students filled out a survey reflecting on this medium of language teaching and learning. Finally, focus group interviews were conducted to determine students’ views on the use of Instagram as an educational tool in terms of its flexibility and usefulness. The findings of the study suggest that JiT teaching, and PI promoted students’ achievement and enhanced students’ motivation, particularly in relation to fluency and novelty in the second language. Furthermore, students viewed Instagram as a successful educational tool that significantly facilitated their collaboration. Nevertheless, this study also highlights the challenges of using JiT teaching and PI to facilitate language learning. Suggestions for practitioners are specified; including providing adequate e-learning tools for students to enhance language learning motivation and achievement.
Exploring the status of the human operator in Industry 4.0: A systematic review
Authors: Cunha Liliana , Silva Daniel , Maggioli Sarah
Published: September 2022

Industry 4.0 (I4.0) promises to transform jobs and working conditions through the implementation of unprecedented human-machine interaction modes. As the operator working in these new settings, known as the Operator 4.0, is a relatively recent concept, and although technological developments are expected to support workers and require higher labor skills, the risks and health impacts resulting from these changes remain underexplored. This systematic review aims to (i) systematize literature findings on how workers are perceived and participate in I4.0 work systems; (ii) identify the main technological changes driven by I4.0; and (iii) instigate discussion regarding the impacts these changes may have for workers and the sustainability of work systems. Following a systematic review approach using the PRISMA protocol, the articles were organized into two main analysis axes: the technical changes brought about by I4.0, and the representation of the human worker within these new work settings. The findings reveal that a techno-centered approach still seems to be dominant in guiding the implementation of I4.0 models; secondly, as a consequence, the social dimensions of work tend to remain as residual issues, overshadowed by the promises related with technology (e.g., productivity, efficiency); finally, the representation of the Operator 4.0 remains blurry, as he/she is perceived as gender neutral, skillful, and perfectly fit for work, assuring the functioning (and compensating for the limits) of these systems. While I4.0 promises safer and more productive workplaces, issues related to employment conditions, emerging risks and health impacts become more prominent when analyzed from an activity-centered perspective. In terms of future research, a more heuristic analysis could be achieved through a participatory and work-centered approach and following a gender perspective. This way, visibility could be conferred to another side of I4.0, thus guaranteeing conditions for the sustainable development of these work situations.
The impact of psychological distress on problematic smartphone use among college students: The mediating role of metacognitions about smartphone use
Authors: Chen Huohong , Ma Jing , Guan Jinliang , Yin Lin , Shi Zifu , Zhang Yihan
Published: September 2022

A mediation model was constructed to clarify the relationship and mechanisms linking psychological distress to problematic smartphone use (PSU), focusing on the mediating role of metacognitions about smartphone use. A questionnaire method was used to investigate psychological distress, metacognitions about smartphone use, and problematic smartphone use among 664 college students. The results showed that (1) psychological distress had a significant positive predictive effect on problematic smartphone use, and (2) there were differences in the underlying mechanisms linking different types of psychological distress to problematic smartphone use. Specifically, negative metacognition about smartphone use partially mediated the relationship between depression and problematic smartphone use, whereas anxiety could act on problematic smartphone use through the parallel mediation of positive metacognition about smartphone use and negative metacognition about smartphone use, with the latter having a greater positive mediating effect than the former. These findings reveal the mechanism of action linking different types of psychological distress to problematic smartphone use from the perspective of the cognitive-behavioral model of pathological Internet use, which has implications for the prevention and intervention of problematic smartphone use among college students.
Rethinking mental health wellness among adolescents living with HIV in the African context: An integrative review of mental wellness components
Authors: Orth Zaida , Van Wyk Brian
Published: September 2022

Objective

Adolescents living with HIV (ALHIV) are considered to be at heightened risk for developing mental health problems in comparison to their peers due to the burden of living with a stigmatized condition and managing a chronic condition. Poorer mental health outcomes among ALHIV are associated with lower rates of adherence to anti-retroviral therapy (ART). It is necessary to improve mental wellness among ALHIV as this acts as a buffer against developing mental health problems which, if left untreated can evolve into mental health disorders. Research on mental wellness concepts among ALHIV is underdeveloped which is associated with a lack of appropriate measures of mental wellness. We conducted an integrative review to conceptualize mental wellness and consider the critical components for measuring mental wellness in ALHIV.

Method

An integrative review of published literature focusing on mental wellness of ALHIV in the African context was conducted. The process was guided by the PRISMA operational steps. As part of our problem identification phase, we drew on findings from a previous systematic review of mental wellness instruments and a qualitative photovoice study on exploring the experiences of ALHIV, to develop an initial framework of 13 mental wellness concepts and behaviors which informed the search strategy.

Results

The review included 17 articles from which we identified six mental wellness concepts: Connectedness, Sense of Coherence (SOC), Self-esteem, Self-acceptance, Hope for the Future and Spirituality as well as six behaviors facilitating mental wellness: Coping, Resilience, Purpose in Life (goals), Self-efficacy, Adherence Self-efficacy, and Leisure Activities. All of these concepts and behaviors have been noted in our previous research (systematic review and qualitative work), with the exception of adherence self-efficacy. Based on the findings from this review and our previous work, we adapted the Salutogenic Model of Health developed by Antonovsky in 1987, to propose a Salutogenic Model of Mental Wellness (SMoMW) for ALHIV in the African context. This SMoMW may be used to develop an age and culturally appropriate measure of mental wellness for ALHIV.

Conclusion

The findings from this review used to conceptualize mental wellness among ALHIV which can be used to develop a measurement of mental wellness.
High fluency can improve recognition sensitivity based on learned metacognitive expectations
Authors: Esser Sarah , Lustig Clarissa , Haider Hilde
Published: September 2022

Fluency of processing has shown to influence recognition judgments. Fluency most commonly induces a liberal response bias to judge fluently processed information as well-known because knowledge of a high correlation between the frequency of encounters, memory strength, and thus fluency of processing has been acquired in the past. In this study, we aimed to show that high fluency can increase recognition judgment sensitivity as well if the participants had encountered fluent and non-fluent processing during training. Thirty-three participants have been trained with a 12-element sequence in a serial reaction time task. During training, the response stimulus interval alternated block-wise between constant (fluent) and variable (non-fluent). Participants showed a higher capability of discriminating between old and new test sequences under fluent than under non-fluent test conditions. Furthermore, participants did not show any liberal or conservative bias after they have been trained with alternating fluency.
How much consciousness is there in complexity?
Authors: Koculak Marcin , Wierzchoń Michał
Published: September 2022

The notion of complexity currently receives significant attention in neuroscience, mainly through the popularity of the Integrated Information Theory (IIT). It has proven successful in research centred on discriminating states of consciousness, while little theoretical and experimental effort was directed toward studying the content. In this paper, we argue that exploring the relationship between complexity and conscious content is necessary to understand the importance of information-theoretic measures for consciousness research properly. We outline how content could be experimentally operationalised and how rudimental testable hypotheses can be formulated without requiring IIT formalisms. This approach would not only allow for a better understanding of aspects of consciousness captured by complexity but could also facilitate comparison efforts for theories of consciousness.
Most and least important attributes for domestic travel: A best-worst scaling approach
Authors: Lee Soyeun Olivia , Kim JooHyang , Han Heesup
Published: September 2022

This study identifies the most important and least important selection attributes in Korean domestic travel during the COVID-19 pandemic. A total of 632 responses were used as the final analysis by conducting a survey of Koreans who have experienced travel in Korea since the outbreak of COVID-19. In order to explain tourists’ domestic travel selection preferences, best-worst scaling was used. As a result of the study, it was found that the destination environment is most important and is considered relatively important in the order of accommodation, major activities, expenditure, and crowdedness. On the other hand, length of stay, transport mode, travel time, and media exposure showed relatively low importance. In a situation where overseas travel is unstable due to the risk of infectious diseases, it is urgent to identify changes in domestic travel trends/factors that are important to tourists and respond to their needs and expectations. This study is academically expanded in that it not only bridges the research gap that previous studies have missed but also considers and ranks the importance of domestic travel factors at the same time.
Understanding the paradox between tragedies of the commons and the anticommons: From a cognitive psychology perspective
Authors: Yang Xiaowei , Yang Shiqi , Wang Yuejin , Zhang Shuyu , Luo Songmei
Published: September 2022

The tragedy of the commons refers to the overuse of resources which are rival in consumption but lack excludability and it also refers to rent dissipation. While the tragedy of the anticommons is a tragedy closely connected with underuse of resources that are rival in consumption and with too strong excludability. The prior studies proved that the tragedy of the commons and the tragedy of the anticommons are symmetric from the perspective of pure mathematics, especially the game theory, which was later refuted by behavioral economics experiments. According to them, the tragedy of the anticommons is severer than the tragedy of the commons. The asymmetry of the tragedy of the commons and the tragedy of the anticommons is a paradox by these different research methods. This paradox shows that there are imperfections in the completely rational economic man hypothesis set up by neoclassical economics. As a fundamental theory, the tragedy of the commons is quite influential in many disciplines, such as microeconomics, public sector economics, ecological economics, environmental economics, management, sociology, property law, and political science. And the tragedy of the anticommons theory has also opened its door of both theoretical research and practical implications since its acceptance by Nobel laureate Buchanan, the main founder of public choice school. Only when theoretical issues are thoroughly discussed and made clear enough, can people avoid misunderstanding or misusing the commons theory. Thus, it is necessary to elucidate the paradox between them. Based on Simon’s bounded rationality, Kahneman and Tversky’s prospect theory, value function, Thaler’s mental accounting, endowment effect, and other cognitive psychological tools, this study clearly shows that agents’ decision-making process is not just based on the long-believed marginal benefit and marginal cost analysis advocated by traditional neoclassical economists. Agents’ decision-making is a process in which agents selectively absorb, code the objective marginal revenue and marginal cost, and feed relevant information to their brain. Therefore, what plays a directly decisive role is not the objective marginal revenue and marginal cost per se, but the mentally perceived subjective utility of marginal revenue and marginal cost by the human brain. Followed by this research clue, the paradox between the tragedy of the commons and the tragedy of the anticommons is elucidated from the perspective of cognitive psychology.
Variants in the zinc transporter-3 encoding gene (SLC30A3) in schizophrenia and bipolar disorder: Effects on brain glutamate–A pilot study
Authors: Jelen Luke A. , Green Mark S. , King Sinead , Morris Alex G. , Zhang Xinyuan , Lythgoe David J. , Young Allan H. , De Belleroche Jacqueline , Stone James M.
Published: September 2022

Zinc transporter 3 (ZnT3) has been implicated in the aetiopathology of schizophrenia. In this pilot study, we tested the hypothesis that the presence of a minor allele of two variants in the gene encoding ZnT3 (SLC30A3) affects brain glutamate and cognitive activity in patients with schizophrenia and bipolar affective disorder. Fifteen patients with schizophrenia (SCZ), 15 with bipolar affective disorder type 2 (BD), and 14 healthy volunteers (HV) were genotyped for two SLC30A3 single nucleotide polymorphisms (rs11126936 and rs11126929). They also underwent structural and functional MRI (n-back) imaging as well as static (PRESS) and functional magnetic resonance spectroscopy (n-back) on a 3 Tesla MRI system. SCZ with at least one copy of the minor allele showed reductions in dorsal anterior cingulate cortex glutamate during the n-back task, whereas SCZ without the minor allele showed an increase in glutamate. BD with the minor allele had reduced glutamate in the anterior cingulate cortex (p < 0.05). There was no effect of SLC30A3 genotype on BOLD activation during n-back or on cortical brain volume. This study supports the further investigation of SLC30A3 and its role in glutamatergic neurotransmission and in the neuropathology of mental illness.
Continuity of mental health care during the transition from prison to the community following brief periods of imprisonment
Authors: Browne Christie C. , Korobanova Daria , Chemjong Prabin , Harris Anthony W. F. , Glozier Nick , Basson John , Spencer Sarah-Jane , Dean Kimberlie
Published: September 2022

Purpose

The prison-to-community transition period is one of high risk and need, particularly for those with mental illness. Some individuals cycle in and out of prison for short periods with little opportunity for mental health stabilization or service planning either in prison or the community. This study describes the socio-demographic, clinical and criminal justice characteristics of individuals with mental illness and frequent, brief periods of imprisonment, examines continuity of mental health care between prison and the community for this group, and reports on their post-release mental health and criminal justice outcomes.

Design/methodology/approach

This study examined a sample of 275 men who had recently entered prison in New South Wales (NSW), Australia, who had been charged with relatively minor offenses and had been identified on reception screening as having significant mental health needs. Baseline demographic and mental health information was collected via interview and file review and contacts with the prison mental health service were recorded for the period of incarceration. Follow-up interviews were conducted 3 months post-release to determine level of health service contact and mental health symptoms. Information on criminal justice contact during the 3 month period was also collected.

Findings

The majority (85.5%) of the sample had contact with a mental health professional during their period of incarceration. Mental health discharge planning was, however, lacking, with only one in 20 receiving a referral to a community mental health team (CMHT) and one in eight being referred for any kind of mental health follow-up on release. Of those followed up 3 months post-release (n = 113), 14.2% had had contact with a CMHT. Of those released for at least 3 months (n = 255), one in three had received new charges in this period and one in five had been reincarcerated.

Conclusion

Continuity of mental health care for those exiting prison is poor, particularly for those with mental health needs experiencing brief periods of imprisonment, and rates of CMHT contact are low in the immediate post-release period. These findings suggest a need for early identification of individuals in this group for timely commencement of intervention and release planning, and opportunities for diversion from prison should be utilized where possible.