bluuebunny/crossref_metadata_2025 huggingface dataset:
STRATEGI PEMASARAN HASIL PENGELOLAAN HASIL BANK SAMPAH SAKURA PALEMBANG DI MASA PANDEMI COVID-19
Authors: Okfarianti Trisa , Kusnadi Kusnadi , Murdiati Eni , Hidayat Hidayat , Karerek Karerek
Published: May 2023

This research is motivated by what happened at the Sakura Waste Bank, apart from dealing with other similar waste banks, they also faced unstable conditions due to the Covid 19 Pandemic. This caused sales at the waste bank to experience significant declines. With the issuance of government regulations not to leave the house, of course this has a big impact. This will hinder the marketing process of the Sakura waste bank, which can normally carry out marketing activities directly to consumers, but with this crowd restriction, the waste bank cannot do so, so it has to change its marketing strategy in order to survive during the current pandemic. With the formulation of the problem, namely (1) What are the obstacles faced by the Sakura Garbage Bank during the pandemic? (2) what marketing strategy was implemented by the Sakura Waste Bank during the Pandemic? This research uses descriptive qualitative which consists of observation, documentation and interviews as data collection techniques sourced from primary data and secondary data. The data analysis technique uses data reduction, data presentation and conclusion drawing. From the results of the implementation of this study, implementing a marketing strategy at the Sakura Palembang Waste Bank during the pandemic, namely using a SWOT analysis marketing strategy. The inhibiting factor of this research is the lack of awareness of human resources including millennials towards the Waste Bank business.

 Keywords: Marketing Strategy, Marketing, Waste Bank, Covid -19
ANALISIS FAKTOR YANG MEMPENGARUHI JAMAAH UMRAH DALAM MENGAMBIL KEPUTUSAN PENGGUNAAN TRAVEL UMRAH FAIRUZ TOUR PALEMBANG
Authors: Sari Wulan Purnama , Nuraida Nuraida , Darmawan Candra , Nasution Nurseri Hasnah
Published: May 2023

The problem taken from this research is that the high number of Umrah pilgrims each year causes the number of pilgrims on the Fairuz Tour Palembang Umrah travel to fluctuate. This is because when making decisions on the use of Umrah travel, many Umrah travel services do not meet regulations, so many factors are taken into consideration by pilgrims. This study aims to determine whether there are individual factors, environmental factors, and marketing stimulus factors that influence Umrah pilgrims in making decisions about using Fairuz Tour Palembang Umrah travel. In this study, researchers analyzed the factors that influence Umrah pilgrims in making decisions about the use of Fairuz Tour Palembang Umrah travel. Respondents in this study were pilgrims who had performed Umrah using Fairuz Tour Palembang Umrah travel with the use of the data used in this study, namely primary data obtained directly from respondents by filling out questionnaires. Then analyze the data obtained in the form of quantitative analysis. Quantitative analysis includes validity test, reliability test, classical assumption test, multiple linear regression analysis, with partial test (T test), simultaneous test (F test), and determinant test (R2). with a significance level of 0.1. From the results of this study indicate that all the independent variables tested have a positive and significant effect on consumer decisions with the F test and t test while the Adjusted R Square number of 0.414 indicates 41.4% variation in consumer decisions obtained from the three independent variables where the remaining 68, 6% is explained by other factors outside this study. 

Keywords: Individual Factors, Environmental Factors, Marketing Stimulus Factors, Consumer Decision
TINJAUAN PERAN PEMIMPIN PERUBAHAN DALAM ORGANISASI
Authors: Saiful Saiful
Published: May 2023

Rosalynn Carter (pakdosen.co.id) revealed that a leader brings other people from a place they want to go, someone who brings their supporters, followers to a place they may not want to go, but who must lead them to achieve the goals they want. go". Therefore, the progress of the organization can be seen from the changes made, of course the one who makes the changes is one of the leaders in the organization, because the leader is able to move all elements of the organization, to achieve results in accordance with the objectives. The change does not stand alone, but there are various factors that determine the process of change that is carried out. In general, there are factors from within the organization and factors from outside the organization, among which is the leader factor, where the role of the leader is to make changes to the organization he leads. A leader is also seen from controlling a change he does, a control that is the core of the leadership role in the organization. Change has pros and cons, for those who are contra because their position will change to feel uncomfortable because of changes in the organization, while for the pros feel the need for organizational change in order to achieve more goals than what has been achieved so far
STRATEGI MANAJEMEN SOSIALISASI PENYELENGGARAAN HAJI DAN UMRAH PT FAJRI TOUR TRAVEL TANGERANG
Authors: Rafiudin Ahmad , Aini Silvia Qurrotul
Published: May 2023

The problems studied in this thesis are: How is the Umrah Jamaat Socialization Strategy carried out by Travel Indonesia PT. The purpose of this research is to find out the form of strategy in socializing with Umrah pilgrims at PT Fajri Tour Travel Tangerang. facts and data regarding the form of the Umrah Jama'at Socialization Strategy at PT Fajri Tour Travel Tangerang, then the data is described, analyzed and discussed to answer these problems. From the results of this study it was found that the strategy used by PT. Fajri Tour Travel Tangerang is an Intensive Strategy, which includes product development and increased product sales. This form of development is adapted to situations and conditions with a dynamic form, one of its characteristics is that marketing implementers can be creative according to the shape of the market they are facing. So with this dynamic form of development, it is also hoped that the increase in product sales has the opportunity to increase consumer acquisition from time to time. While the socialization process is carried out in collaboration with institutional parties, such as the taklim assembly, Islamic holidays and other events related to program socialization, and also always carries out socialization by providing good services, such as office services and when doing activities. socialization in agencies or institutions.

Keywoard: Management, Travel, Socialization
2D Simplified Wildfire Spreading Model in Python: From NumPy to CuPy
Authors: San Martin Daniel , Torres Claudio
Published: May 2023

Wildfires are a problem of great interest in the world because of the damage they cause every year to forest, wild fauna and flora, an also threatening human lives, among others. There are some computational models for numerical simulations to address this phenomena, and a few of them have an open-source implementation. The goal of this work is to extend a CPU Python’s implementation of wildfire open-source framework developed in NumPy, to a GPU improved version using CuPy. The algorithm used is based on a numerical discretization of a wildfire spreading mathematical model described by a system of partial differential equations. Computational and mathematical components, numerical simulations and applications are described in details in the document. In addition, this work includes includes a brief performance comparison between both implementations, pointing out that we can achieve good execution times using the CuPy GPU implementation without spending enough programming effort.
Susanna e os anciãos: análise comparativa das obras de Artemísia Gentileschi (1610) e Jan Both (1642)
Authors:   Faculdade Arquidiocesana de Mariana, Pereira Débora de Viveiros
Published: May 2023

Este trabalho tem como objetivo analisar comparativamente duas obras com o mesmo tema, executadas por artistas distintos, a partir de tais conceitos dualistas: “celestial x mundano”, “prazer x dor” e “esperança x desespero”. As obras escolhidas contemplam a temática bíblica da história de “Susana e os anciãos” segundo a visão de Jan Both e Artemísia Gentileschi.
A iconografia dos doges venezianos, os rituais e as construções arquitetônicas de Veneza segundo a obra de Cesare Vecellio
Authors:   Universidade Estadual de Campinas, Carvalho Larissa Sousa de
Published: May 2023

Em "De gli habiti antichi, et moderni di diuerse parti del mondo" (1590), Cesare Vecellio dedica 118 gravuras para sua gloriosa Veneza (séculos XII-XVI). O autor toma a liberdade de acrescentar comentários que abstraem o exame do vestuário para abordar outras questões, como perspectivas da Praça de São Marcos, explicações sobre a forma de governo, rituais, entre outros aspectos da Serenissima. Analisaremos o seu conteúdo escrito e teceremos comparações com a arte veneziana do período, utilizada como sua legitimadora. Além disso, a iconografia dos doges será cotejada e aprofundada, mediante considerações sobre o contexto em que tal personagem se inseria e demais relações possíveis.
Pintores coloniais nas minas setecentistas: a vez de Manoel Ribeiro Rosa
Authors:   Universidade Federal de Minas Gerais, Rezende Leandro Gonçalves de , Leopoldino Armando Magno de Abreu ,   Universidade Federal de Minas Gerais
Published: May 2023

Nas últimas décadas, a produção historiográfica sobre as artes nas Minas Setecentistas cresceu substancialmente, criando e destruindo mitos, enaltecendo e desvalorizando gênios. O olhar sobre o patrimônio artístico mineiro, principalmente sobre a arte sacra, ainda conserva o encanto daqueles primeiros modernistas, que, em meados do século XX, aprofundaram seus estudos no almejo de valorizar aquela que seria “uma manifestação genuína da Arte Brasileira”. Criaram o conceito de Barroco Mineiro cujo ápice estaria na produção de Aleijadinho e Ataíde. No entanto, uma leva de bons escultores, entalhadores, pintores, pedreiros, enfim, uma leva de artistas e artífices não foi contemplada por essas pioneiras pesquisas.
O laboratório de mídias locativas (laloca) e a pesquisa histórica: mapeamento das obras arquitetônicas do estilo art déco em Juiz de Fora
Authors:   Universidade Federal de Juiz de Fora, Reis Filho Lúcio , Suppia Alfredo ,   Universidade Federal de Juiz de Fora
Published: May 2023

Esta comunicação visa divulgar o trabalho do Laboratório de Mídias Locativas e Cinema GPS (LaLoca), baseado no Instituto de Artes e Design da Universidade Federal de Juiz de Fora. Primeiramente, trataremos da relevância do estilo art déco na arquitetura de Juiz de Fora, Minas Gerais. Em seguida, passaremos a considerações sobre o fenômeno das mídias locativas e, por fim, procederemos a um breve estudo de caso, um relato do projeto de documentário locativo dedicado ao mapeamento das edificações em estilo art déco na cidade. Em síntese, propomos analisar o recurso às mídias locativas (locative media) enquanto ferramenta de pesquisa e divulgação do conhecimento histórico.
Inversões conceituais: a publicação como espaço de exposição no campo ampliado
Authors:   Universidade Federal do Espírito Santo, Oliveira Silfarlem Junior de
Published: May 2023

Seguindo o curso das transformações, inquietudes e ampliações tanto do objeto quanto do próprio campo da arte por volta dos anos 60 e 70 do século passado (acompanhando a revolução nos meios de comunicação), as experiências artísticas conceituais, processuais e performativas irão adotar outros “espaços expositivos”, bem como, em muitos casos, problematizar os papéis definidos anteriormente na produção do objeto autônomo, do curador, do crítico e do artista. Neste sentido, o texto aqui apresentado centrar-se-á na publicação como espaço expositivo e, ao mesmo tempo, como obra (objeto) de arte.
Thornton dial: fora do quê?
Authors:   Saint Louis University, Dennis Whitney
Published: May 2023

Este trabalho pretende expor algumas dificuldades que existem para os artistas que não se enquadram facilmente nas categorias principais da história da arte, usando o exemplo de Thornton Dial. A arte do Thornton Dial, e sua carreira junta com Bill Arnett, seu patrono, propõe muitas perguntas, a muito variadas. Este trabalho tenta pesquisar sobre algumas delas, principalmente sobre a categorização do artista, e a dificuldade na comprensão dele usando metodos convencias da historia da arte. O fin desta pesquisa deverá lançar alguma luz sobre os fatores que mantiveram à Dial fora das instituções do corrente principal, e analisar porque é que Dial e outros artistas como ele, são muitas vezes deixados de fora do famoso Cânon de arte elevado. Também se examinara o progresso obtido pelo principal promotor Dial, Bill Arnett, através da sua determinação implacável para obter reconhecimento para Dial e ver sua inclusão nos principais instituições de arte. É importante notar que os acontecimentos históricos que são mencionados aqui são simplesmente destacados por sua relevância para este trabalho, e de modo nenhum abrangem a totalidade da longa e complexa história do Dial e Arnett juntos.
No need to renew: The end of Japan’s teacher license renewal system and the future
Authors: Chapple Julian
Published: May 2023

In 2007, the Japanese Ministry of Education revised the Education Law to pave the way for the introduction of a teaching license renewal system in 2009. The reform required all teachers to complete at least 30 hours of training in specially tailored university courses as a prerequisite for renewal of their teaching licenses every 10 years. In late 2021, however, the government abruptly announced it was ending the scheme in 2022 in a policy about face reminiscent of the abolition of yutori kyōiku (“education without pressure”) in 2007. This qualitative study aims to examine the background, outline and issues of Japan’s license renewal system in order to understand the reasons for its demise and illustrate the similarities with the yutori kyōiku experiment. Drawing on interview data and reflections from the author’s experiences with the system over 12 years, it looks at some of Japan’s unique training opportunities and, employing examples from other countries, examines what kind of ‘training’ is needed moving forward. It concludes by calling for a widening of the interpretation of ‘training,’ and indeed, the license itself, so as to increase diversity in what is a considerably restrictive employment sector.
The Introduction and Implications of Teacher AI Education Policy in China
Authors:   The Association of Korea Counseling Psychology Education Welfare, PARK Young-Jin
Published: May 2023

Now that global attention is paid to AI education, China is aggressively implementing AIeducation for teachers at the government level. China which induced AI education early to theregular curriculum is producing teachers through teacher training and re-education courses. Thisstudy investigated the introduction and current status of teacher AI education in China mainlywith both nonconfidential and confidential documents and analyzed the characteristics of it. Inthat process, this researcher examined problems related with teacher supply and demand andthe policy of flexibility in different school levels and also analyzed feasibility and problemsassociated with the placement of applicants for teacher training and the plan to appointnonregular teachers. Korea requires a systematic approach to expanding AI education andtraining related teachers from now on. To reduce problems that may occur in it, this authorintended to derive implications from the cases in China regarding the sufficient input ofbudgets, the matter of political use, teachers’ voluntary participation and support from theMinistry of Education, or connection with career education. This study was written mainlythrough literature review, but expert interviews were done, too, partly.
Análisis de las propiedades psicométricas de la Brief Resilience Scale (BRS) en universitarios peruanos
Authors: Escudero Nolasco Juan Carlos , Bracamonte Acasiete Génesis Carolina , Canales Yauri Marycielo , Domínguez Bohórquez Rosa Patricia , García Pérez Geraldine Janeth
Published: May 2023

Esta investigación de tipo instrumental tuvo como objetivo analizar las evidencias psicométricas de la Brief Resilence Scale (BRS) en universitarios peruanos. Participaron 468 estudiantes de universidades públicas y privadas, siendo 62,6% mujeres y 37.4% varones, con un rango de edad entre los 18 a 50 años (M=23.97; DE=6.58). Se ratificó el ajuste del modelo oblicuo mediante el análisis factorial confirmatorio (CFI=.993; TLI=.987; RMSEA=.004; SRMR=.023) y se obtuvieron valores adecuados de confiabilidad (α =.79 y ω =.85). Además, se determinó la validez de criterio en relación con la misma variable con la Brief Resilent Coping Scale (BRCS) (r=.606); para la validez convergente y divergente se empleó el Life Orientation Test (LOT – R) (r=.617) y la Depression Anxiety Stress Scale-21 (DASS – 21) siendo los coeficientes de correlación de r=-.546 en depresión, r= -.515 en ansiedad y r= -.514 en estrés. Finalmente, se analizó la invarianza según sexo, edad y universidad de los participantes, encontrándose adecuados valores de equidad en cuatro niveles de invarianza factorial. Por lo tanto, se concluye que la BRS es un instrumento con adecuadas evidencias de validez, confiabilidad y equidad para medir la resiliencia en universitarios peruanos.
Blockchain-Enabled Quality Improvement Digital Twin for Clinical Trials
Authors: Vatankhah Barenji Reza , Wbrahimi Hariry Reza
Published: May 2023

Clinical trials are research studies performed on people that are aimed at evaluating a medical, surgical, or behavioural intervention. It is a primary way that researchers find out if a new treatment, like a new drug or diet, or medical device is safe and effective in people. In this paper blockchain technology is embedded in a digital twin aiming to improve the quality of clinical trials through the boost of data integrity among the quality-related activities, and to promote participants' safety. First, a blockchain-embedded quality-enabled digital twin is proposed and the interactions among the enablers and peers are highlighted. Then, a prototype of the proposed system is developed, and the data of a pilot trial is used to justify the applicability of the system. The results showed that the proposed system is efficient, and hence it is feasible to adopt into a clinical trial. With the successful development of the proposed system, it is promising to provide effective data integration and knowledge management for improving participant safety.
Cytoplasmic Membrane Homeostasis is Important for the Pathogenicity of Pathogenic Fungi
Authors: Peng Yuejin , Chen Bin
Published: May 2023

The cytoplasmic membrane is the fundamental component of all living cells, which participates in various physiological processes, such as material exchange, stress response, cell recognition, signal transduction, cellular immunity, apoptosis, pathogenicity, etc. The normal function of a cytoplasmic membrane requires stable organization of transmembrane protein-lipid microdomains, transmembrane protein-cell wall microdomains, and cytoskeleton-transmembrane protein microdomains. Here, we review the mechanisms and functions of various membrane lipid components, fatty acid content and saturation, membrane curvature, and cell wall and cytoskeleton in plasma membrane homeostasis affecting the pathogenicity of pathogenic fungi. Pathogenic fungi maintains plasma membrane homeostasis and contributes to fungal virulence by maintaining plasma membrane assembly, structural and functional integrity of pathogenic fungi at various stages of cell development through interactions among lipid components of cytoplasmic membranes, transmembrane proteins, cytoskeleton and cell wall components, etc.
Mississippi River climate model–based hydrograph projections at the Tarbert Landing location
Authors: LaHatte W. , Tavakoly Ahmad , Lytle Sara , Lewis James
Published: May 2023

To better understand and prepare for the possible effects associated with potential climate changes on the lower Mississippi River, the State of Louisiana Coastal Protection and Restoration Authority sought information on the historical, current, and projected future hydrodynamics of the Mississippi River. To this end, flow duration curves (FDC) for the Tarbert Landing location were generated, based on climate models derived from two of the four scenarios of the Coupled Model Intercomparison Project, Phase 5 (CMIP5), multimodel ensemble representative concentration pathways (RCPs). The global CMIP5 datasets were used by the variable infiltration capacity land surface model to produce a runoff dataset, using a bias-correction spatial disaggregation approach. The runoff datasets were then applied to simulate streamflow using the Routing Application for Parallel computatIon of Discharge (RAPID) river routing model. Based on the streamflow, FDCs were calculated for 16 CMIP5 as well as observed historical data at the Tarbert Landing location. Key observations from the results are that the 90th percentile exceedance of the simulated versus the observed flows is more frequent for the RCP 8.5 scenario than for the RCP 4.5 scenario and that the maximum annual flows for the RCP 8.5 scenario are generally smaller than for the RCP 4.5 scenario.
Diurnal variation in methane emission from a rice paddy due to ebullition
Authors: Kajiura Masako , Tokida Takeshi
Published: May 2023

The diurnal variation in methane (CH4) emission commonly observed in rice paddies has been linked to changes in air and soil temperatures. The temperature dependence is not simple, however, and the mechanisms underlying it in relation to the diurnal variation are still poorly understood. Here, we investigated the diurnal variation of CH4 flux and its relationship with air temperature in a Japanese paddy by dividing the total flux into two major pathways, plant-mediated and bubbling emission. The diurnal variation of total flux was minimal at the early parts of reproductive growth (panicle formation and booting stages), when plant-mediated emission was dominant. At a later stage (heading period), ebullition accounted for 43–70% of the total emission, and CH4 emission via both pathways varied diurnally; however, the diurnal range was much greater for bubbling emission (ratio of max. to min. = 3.5) than for plant-mediated emission (ratio of max. to min. = 1.4). The Bubbling emission depended on the temperature, but the emission was greater during the flux-increasing phase in the morning than the flux-decreasing phase in the afternoon even at the same temperature, forming a hysteresis in the flux versus temperature relationship. These results indicate that abiotic processes, such as a change in the solubility of gases and the temperature dependence of the gas-phase volume (Charles’s law), may play a central role in shaping the diurnal pattern of the CH4 ebullition.
MIR210HG may promote the progression of preeclampsia through CDHR5
Authors: Sun Ningxia , Zhang Lu , Xu Jine , Han Mengmeng , Chen Aiping , Liu Shiguo
Published: May 2023

Introduction:Because early diagnosis and timely detection of preeclampsia (PE) remains challenge. This study aims to explore the differential expression and pathogenic mechanism of the lncRNA MIR210HG in peripheral blood, which will lay the basis for its use as a biomarker of PE.
Methods: Total RNA was extracted from the peripheral blood of 88 women with preeclamptic pregnancies and 81 women with normal pregnancies. The MIR210HG expression levels were determined by RT-PCR and western blot. SiRNA and MIR210HG overexpression plasmids were constructed and transfected into human trophoblast and vascular endothelial cells.
Results: The lncRNA MIR210HG was significantly upregulated in the peripheral blood of patients with PE compared to the control group. Additionally, the expression level of MIR210HG in patients with early-onset PE was significantly higher than that of the control and late-onset PE groups. CDHR5, identified by bioinformatics predictions after RT-PCR confirmation, was upregulated in placental tissue compared to the control group. Furthermore, cytology experiments confirmed that knockdown of MIR210HG also decreased CDHR5 in human trophoblast and vascular endothelial cells. After knockdown of CDHR5, expression of MIR210HG was also reduced.
Discussion: The lncRNA MIR210HG is highly expressed in the peripheral blood of PE, indicating MIR210HG may become a potential biomarker for the diagnosis and prediction of PE. In addition, MIR210HG may interact with CDHR5 to jointly promote the progression of PE. However, further experiments are needed to confirm the pathogenic mechanism.
An exploratory study of international tourists’ engagement in Māori indigenous tourism
Authors: Kheiri Jay , Nasihatkon Violet
Published: May 2023

The authors have requested that this preprint be removed from Research Square.
An operational matrix method based on scalingfunction of Daubechies wavelet to solve stochastic differential equations
Authors: Shariati Nasim Madah , Yaghouti Mohammadreza , Alipanah Amjad
Published: May 2023

In our joint work, by introducing Quasi Block-Pulse (QBP) functions, we provide integration and stochastic integration operational matrices based on scaling function of Daubechies wavelet to solve linear and nonlinea rstochastic differential equations. Due to interesting properties of the Daubechies wavelet such as orthogonality, compactly support and vanishing moments, these operational matrices have good properties. The convergenc eof the proposed method is presented and in order to verify the accuracy of the proposed method some numerical examples are provided.
Female sexual response to audiovisual stimuli in 2D/3D modality and first/third person perspective stance
Authors: Marečková Alena , Hůla Martin , Klapilová Kateřina , Novák Ondřej
Published: May 2023

The study investigates the effect of sexually explicit video stimuli displayed in a different modality (3D vs. 2D) and perspective (first vs. third person) on female sexual arousal, both subjective and genital, valence ratings, and sexual and general presence. We exposed 47 heterosexual female participants to four stimuli, two displayed on a flat screen and two using a VR headset. We used almost identical videos in both modalities and perspectives, thus eliminating any confounding variables caused by using different videos. We measured genital arousal via vaginal photoplethysmography (VPG) and subjective responses using questionnaires. We did not find any differences in modality or perspective in genital arousal. 2D videos were considered more subjectively arousing than the 3D videos (d = 0.33, p = 0.02). 2D videos and third-person perspective videos elicited stronger feelings of sexual (2D/3D: d = 0.86, p < 0.001; first/third person: d = 0.50, p < 0.001) and general presence (2D/3D: d = 1.09, p < 0.001; first/third person: d = 0.75, p < 0.001 ) than 3D videos and first-person perspective videos. Our findings are in discord with the previous studies. We discuss the possible causes of the observed differences.
Analysis of the Most Probable Exit Path in the synthetic gene network with genetic toggle
Authors: Guo Zhuqin , Xu Wei , Zhang Wenting
Published: May 2023

In the process of the development of biotechnology, the research on the manipulation and regulation of biochemical molecules such as genes and proteins has become a hot direction. In particular, the synthetic gene network with genetic toggle has attracted the attention of many scholars due to its great creative space and strong operability. However, the current research on synthetic gene network with genetic toggle mainly focuses on the influence of noise on the stability of the system and stochastic resonance. Little research has been done on exploring the switching process of the system with genetic toggle under noise excitation. In this paper, the most probable exit path(MPEP) is proposed to understand the switching of genetic toggle in the system. Firstly, we studied the MPEP of protein concentration switching in a bistable synthetic gene network with genetic toggle in E.coli organism when noise intensity is very small. Large deviation theory is used to explore the MPEP of protein concentration switching from a new perspective. Secondly, OM action functional is introduced to get the MPEP of the system under Gaussian white noise excitation. It is revealed that two independent noises have opposite effects on protein concentration switching. Finally, the MPEP is shown by energy landscape, and it is found that the reduction of noise intensity is not conducive to protein concentration switching.
EphrinA5 regulates cell motility by modulating lncRNA/DNA triplex-dependent targeting of DNMT1 to the Ncam1 promoter
Authors: Yildiz Can Bora , Kundu Tathagata Tathagata , Gehrmann Julia , Koesling Jannis , Ravaei Amin , Jakovcevski Mira , Pensold Daniel , Zimmerman Olav , Rossetti Giulia , Costa Ivan G. , Zimmer-Bensch Geraldine
Published: May 2023

Background:
Cell-cell communication is mediated by membrane receptors and their cognate ligands, such as the Eph/ephrin system, and dictates physiological processes, including cell proliferation and migration. However, whether and how Eph/ephrin signaling culminates in transcriptional regulation is largely unknown. Epigenetic mechanisms are key for integrating external “signals”, e.g., from neighboring cells, into the transcriptome. We have previously reported that ephrinA5 stimulation of immortalized cerebellar granule (CB) cells elicits transcriptional changes of lncRNAs and protein-coding genes. LncRNAs represent important adaptors for epigenetic writers through which they regulate gene expression. Hence, we here aimed to investigate, whether ephrinA5 can act on gene transcription through modulating lncRNA-mediated targeting of the DNA methyltransferase 1 (DNMT1) to gene promoters, thereby regulating cell motility.
Results:
We analyzed the interaction of lncRNA with protein-coding genes by the combined power of in silico modeling of RNA/DNA interactions and respective wet lab approaches. We found that Snhg15, a cancer-related lncRNA, forms a triplex structure with the Ncam1 promoter and interacts with DNMT1. EphrinA5 stimulation leads to reduced Snhg15 expression, diminished Snhg15/DNMT1 interaction and decreased DNMT1 association with the Ncam1 promoter. These findings can explain the attenuated Ncam1 promoter methylation and elevated Ncam1 expression induced by ephrinA5 stimulation that in turn elicits decreased cell motility of CB cells.
Conclusion:
Based on our findings, we propose that ephrinA5 influences gene transcription via lncRNA-targeted DNA methylation underlying the regulation of cellular motility. Such mechanism could be relevant in the context of cancerogenic processes, known to involve Eph/ephrin signaling and epigenetic remodelling.
The Effectiveness of a Medical Cocktail Therapy for Non-healing Refractory Ischemic Diabetic Foot Ulcers- Case Reports
Authors: Sureshkumar Pichakacheri , Kumar Sidharth S
Published: May 2023

INTRODUCTION:
Diabetic foot ulcers (DFU) are a serious complication of diabetes mellitus that can have significant medical and socioeconomic impact as well as increase in mortality rates. Healing of ischemic or neuro ischemic foot ulcers is most challenging as conventional therapies often fail to achieve adequate vascularity. Ulcers of the heel area can be very severe and strenuous to treat and can eventually end up in the amputation of the limb due to poor vascularity. So optimization of therapies to improve vascularity should be a major objective in successfully treating such cases.
CASE PRESENTATION:
Here we report two cases of chronic non-healing ischemic ulcers of the heel area of the foot. Patients approached us after the conventional medical treatments had failed to achieve healing. We treated them with a cocktail (Diabcare protocol) that we formulated using existing medications along with appropriate wound care and antibiotics. Diabcare protocol comprises plaque regression agents (statins), antiplatelet drugs (Aspirin), novel oral anticoagulant (Rivaroxaban) and vasoactive agents [PDE3 inhibitor (Cilostazol) and PDE5 inhibitor (Tadalafil)]. On Serial measurements, ulcers showed progressive healing and wound contraction. Vascularity improved significantly and fresh granulation appeared within days to weeks of initiation of therapy. Ulcers healed completely, with spontaneous epithelialization in the first case and after SSG in the second case, within a mean period of three months.
CONCLUSION
The ‘Diabcare protocol’ is found to be effective in healing difficult-to-heal ischemic foot ulcers where conventional treatment fails.
Causes of accelerated High-Tide Flooding in the U.S. since 1950
Authors: Sun Qiang , Dangendorf Sönke , Wahl Thomas , Thompson Philip
Published: May 2023

The U.S. coastlines have experienced rapid increases in occurrences of High Tide Flooding (HTF) during recent decades. While it is generally accepted that relative mean sea level (RMSL) rise is the dominant cause for this, an attribution to individual components is still lacking. Here we use local sea-level budgets to attribute past changes in HTF days to RMSL and its individual contributions. We find that while RMSL rise generally explains >90% of long-term increases in HTF days, their spatial patterns also depend on the flooding threshold location and local water level characteristics. Vertical land motion dominates long-term increases in HTF particularly in the northeast, while sterodynamic sea level (SDSL) is most important elsewhere and on shorter temporal scales. We show that the recent SDSL acceleration in the Gulf of Mexico has led to an increase in HTF of more than 220%, a number that is unpreceded both in time and space.
MSFC: A New Feature Construction Method for Accurate Diagnosis of Mass Spectrometry Data
Authors: feng xin , dong zheyuan , Li Yingrui , Cheng Qian , Xin Yongxian , Xin Ruihao
Published: May 2023

Background
Mass spectrometry technology can realize dynamic detection of many complex matrix samples in a simple, rapid, compassionate, precise, and high-throughput manner and has become an indispensable tool in accurate diagnosis. The mass spectrometry data analysis is mainly to analyze all metabolites in the organism quantitatively and to find the relative relationship between metabolites and physiological and pathological changes.
Methods
A feature construction of mass spectrometry data (MSFS) method is proposed to construct the features of the original mass spectrometry data, so as to reduce the noise in the mass spectrometry data, reduce the redundancy of the original data and improve the information content of the data. Chi-square test is used to select the optimal non-redundant feature subset from high-dimensional features. And the optimal feature subset is visually analyzed and corresponds to the original mass spectrum interval. Training in 10 kinds of supervised learning models, and evaluating the classification effect of the models through various evaluation indexes.
Results
Taking two public mass spectrometry datasets as examples, the feasibility of the method proposed in this paper is verified. In the coronary heart disease dataset, during the identification process of mixed batch samples, the classification accuracy on the test set reached 1.000; During the recognition process, the classification accuracy on the test set advanced to 0.979. On the colorectal liver metastases data set, the classification accuracy on the test set reached 1.000.
Conclusion
This paper attempts to use a new raw mass spectrometry data preprocessing method to realize the alignment operation of the raw mass spectrometry data, which significantly improves the classification accuracy and provides another new idea for mass spectrometry data analysis. Compared with MetaboAnalyst software and existing experimental results, the method proposed in this paper has obtained better classification results.
Association of the previous experience of maternal migration absence with non-suicidal self-injury (NSSI) in their children: A cross-sectional study in Anhui, China
Authors: Akezhuoli Hailati , Guo Shi , Zhao Honghui , Xu Jiayao , Xie Qianwen , Zhou Xudong , Lu Jingjing , Li Lu
Published: May 2023

Background
Non-suicidal self-injury (NSSI) emerged as an escalating health concern in youths worldwide, particularly in the vulnerable. In China, a burgeoning internal migration in human history has been tearing families apart, generating a surging number of left-behind children (LBC) with a lack of parental supervision. Despite the empirical acknowledgment of parental migration as a risk predictor of the NSSI among LBC, there remains a lack of understanding of the specific role of the maternal migration experience.
Objective
This study sought to determine the association of maternal migration experience and its characteristics in terms of age at initiation and length with 12-month NSSI among children. Participants & setting: Data were collected by a self-administrated questionnaire survey conducted among a school-based sample of students in the Anhui province, China.
Methods
A total of 2476 participants were analyzed, and the Chi-squared tests and binary logistic regression analysis were employed.
Results
Compared with children whose father is currently migrating and the mother never migrated for work (FM-MN), children whose father never migrated but the mother previously migrated for work (FN-MP, OR = 2.21, 95% CI [1.27–3.84], p < 0.01) and children whose father is currently migrating and the mother previously migrated for work (FM-MP, OR = 1.46, 95% CI [1.00-2.13], p < 0.05) evidenced greater odds of the prevalence of 12-month NSSI. And also, a significantly higher risk for 12-month NSSI was found among FN-MP and FM-MP compared with children whose parents lived in the household and neither had ever migrated for work (FN-MN). For those children with maternal migration experience, while controlling for the paternal migration characteristics, there was no difference between before and after school age at initiation of migration in the risk of NSSI, nor were there any differences across 1–3, 4–6, and more than 6 years of maternal migration length.
Conclusions
Findings suggested the potential long-term effect of the experience of maternal migration on children and provided implications for the early identification and prevention of children at risk for NSSI, particularly within the maternal migrant family context.
Impact of preoperative occult renal dysfunction on early and late prognosis in elderly patients with coronary artery bypass in China
Authors: Liu Zhonghe , Zhang Chong , Zhou Le , Sun Yangyang , Pan Yilin , Han Lixiang , Jin Lei , Li Zhi , Zhang Yangyang
Published: May 2023

Background
Renal dysfunction is related to the poor outcomes of cardiac surgery. Preoperative occult renal dysfunction (PORD) is defined as patients with normal serum creatinine (Scr) but the glomerular filtration rate (GFR) less than 60 ml/min/1.73m2. However, there is no report on prognosis of elderly PORD patients undergoing coronary artery bypass grafting (CABG). This problem deserves further exploration.
Methods
Totally 1103 patients undergoing CABG with normal Scr and age ≥ 70 years old were enrolled. The estimated GFR (eGFR) of each patient was monitored using the Berlin initiative study formula. The patients were divided by eGFR into PORD group and control group. Propensity score matching was used to adjust preoperative characteristics. Univariate and multivariate analyses were performed to identify risk factors for postoperative acute kidney injury (AKI) and postoperative mortality. The Kaplan-Meier method was applied to estimate survival rates. A multivariate cox regression model was used to analyze the impacts of factors on long-term survival.
Results
Elderly patients with normal Scr had high PORD proportion (27.65%). The PORD group had significantly higher preoperative Cr (99.20 ± 12.74 vs. 71.57 ± 11.96), postoperative AKI rates (39.13% vs. 17.75%) and postoperative mortality (5.80% vs. 1.81%) compared with control group. PORD was a significant risk factor for postoperative mortality (OR = 11.648) and postoperative AKI (OR = 2.731). PORD was also a significant risk factor for long-term death after CABG (HR = 1.724).
Conclusions
PORD is a risk factor for postoperative AKI and short- and long-term death in elderly patients undergoing CABG. eGFR shall be used to assess preoperative renal function.
Effects of prefrontal tDCS on working memory performance in chronic alcohol and tobacco use
Authors: Göttgens Franziska , Habel¹ Ute , Wallheinke Paul , Blendy Julie A. , Weidler¹ Carmen
Published: May 2023

Deficits in working memory (WM) are observable in a wide range of those psychiatric disorders associated with decreased activation of the prefrontal cortex. Given that WM is a crucial process supporting various cognitive functions, deficits interfere with daily life tasks and psychiatric treatment. The present study examines the influence of prefrontal brain stimulation on WM performance in alcohol-dependent patients (AD), tobacco users (TU) and healthy controls (HC). In a double-blind, sham-controlled study, transcranial direct current stimulation (tDCS) was applied to upregulate right dorsolateral prefrontal cortex (DLPFC) activity. Participants received anodal tDCS with current at an intensity of 1.5 mA for 20 minutes in the active group. A large reference electrode (10x10cm) was chosen to minimize stimulation effects and situated over the bilateral supraorbital area. While being stimulated, participants performed the n-back task as a measure of WM performance. Results revealed no significant differences in WM performance between active and sham stimulated participants. Further, performance in the AD group did not differ from TU and HC. According to previous research, stimulation the left DLPFC may more effective in enhancing WM performance. Our findings suggest that the use of larger reference electrodes can minimize the impact on underlying brain regions.
Feasibility and Acceptability of Spanish-language Facebook Group on Latino Parents’ COVID-19 Vaccine Beliefs: Case study of the Brigada Digital de Salud
Authors: Andrade Elizabeth L. , Gonzalez Anna I. , Wysota Christina N. , Favetto Carla , Gomez Kaitlyn , Broniatowski David , Abroms Lorien C.
Published: May 2023

Background
COVID-19 vaccine uptake among U.S. Latino adults has been slower than other groups, and younger Latino children continue to be underrepresented among vaccinated populations. Parental vaccine hesitancy has been an important barrier, often stemming from concerns about safety, effectiveness, side effects, and exposure to social media misinformation. The Brigada Digital de Salud was established in May 2021 to address misinformation by disseminating credible, science-based, and culturally-appropriate COVID-19 information in Spanish on social media platforms.
Methods
We conducted a 5-week randomized controlled trial in August-September, 2022 using two private Facebook groups (n = 55 intervention and n = 65 control) to test intervention feasibility and acceptability of COVID-19 vaccine promotion among Spanish-speaking Latino parents. Also of interest were the effects of Brigada Digital content on vaccine beliefs, intentions, and uptake. Intervention participants received daily COVID-19 prevention and vaccination posts with prompts for interaction. All study participants completed baseline and follow-up surveys. Participant engagement was assessed using Facebook analytics.
Results
Feedback from intervention participants regarding content, moderators, and group experience was positive. Participants agreed that posts were informative (4.3/5), trustworthy (4.2/4), and addressed their COVID-19 vaccine concerns (4.2/5). Participants also agreed that moderators were well-informed (4.3/5) and helpful (4.2/5), and they would recommend the group to a friend (4.4/5). Participants remained engaged in the group for the 5-week period, and had, on average, 36.4 post views, 10.6 post reactions, and 3 post comments per participant. Both intervention and control group participants reported greater COVID-19 vaccine-supportive beliefs for adults and children at follow-up; these beliefs increased more among intervention participants, though differences were not statistically significant. When asked about why parent participants had not yet vaccinated their children across all ages, reasons mirrored those reported nationally, with principal concerns being related to potential side effects, safety, and that the vaccine was developed too quickly
Conclusions
This intervention approach shows considerable promise for Latino parents in terms of feasibility, appeal, and appropriateness, and preliminary evidence suggest potential to improve parent COVID-19 vaccine beliefs for adults and children. This intervention approach and content should be further tested with groups of parents who express less supportive COVID-19 vaccine views or have unvaccinated children.
Lambert-Uniform Scan Statistic: Hot Spot Detection of Covid-19 Using a New One-Parameter Scan Statistic
Authors: Abolhassani Ali
Published: May 2023

The spatial scan statistic is widely applied for detection of spatial clusters. These types of clustersare particularly important in epidemiology. Timely warning about the spread of a disease in thesociety can prevent a disaste.Spatial scan statistic can be applied on different types of spatial data to detect the location ofclusters. However, only the two-parameter Beta distribution is conducted on ratios, percentagesand bounded spatial data in the case of hotspot detection which is based on the regression modeland needs explanatory variables.In this study we focus on the recently proposed one-parameter Lambert-Uniform distributionto construct a new scan statistic for spatial bounded data. We present algorithms for detectingirregular and circular spatial clusters based on the new Lambert-Uniform scan statistic. Theadvantages of Lambert-Uniform scan over the Beta scan are at least in two aspects. First, theLambert-Uniform distribution has only one parameter, hence, in the process of clustering, oneneeds to estimate less parameters comparing Beta scan. Second, unlike Beta scan, the LambertUniform scan does not require regression explanatory variables.We consider different scenarios for spatial cluster detection and check the efficiency of LambertUniform scan statistic with the help of common criteria in cluster detection, i.e., precision, recall,bias value, and F 1. We also discuss the importance of the number of data in each cell of themap. We propose to apply MCMC method in sparsely populated maps before performing clusterdetection procedure. The simulation results show that the Lambert-Uniform spatial scan statisticis a suitable method for clustering the bounded spatial data. As a practical example, we considera map of Covid-19 disease and perform clustering based on circular and irregular shape scan.