bluuebunny/crossref_metadata_2025 huggingface dataset:
Cross-sectional study of self-report of health issues in patients who had recovered from moderate-severe COVID-19 in Ho Chi Minh City, Vietnam
Authors: Tran Nguyen Ngoc , Quy Khoa , Pham Huy Ngoc , Van Khanh Trinh Ngoc , Ung Hoang Nguyen Vu , Pham Tung Huy , Cao Viet Quoc , Vuong Lan Ngoc
Published: June 2023

There is increasing recognition that acute coronavirus disease (COVID-19) can be followed by a period of long-term symptoms (“long COVID” or post-COVID-19 syndrome [PCS]). This study investigated health issues in patients who had recovered from moderate-severe COVID-19 in District 10, Ho Chi Minh City, Vietnam.

This cross-sectional study utilized a questionnaire and telephone interviews in patients with confirmed COVID-19 who were treated in hospital between July 27 and September 15, 2021. The questionnaire gathered data on demographics, symptoms during acute COVID-19, and symptoms in the post-COVID-19 period. Logistic regression models were used to identify potential contributing factors with health issues after moderate-severe COVID-19. Health issues after moderate-severe COVID-19, similar to post-COVID-19 syndrome, were defined as the appearance of any signs or symptoms that developed during acute illness or after recovery and persisted for ≥12 weeks and could not be explained by any other medical conditions.

Out of 98 individuals who completed the survey, 72 (73.5%) had symptoms after moderate-severe COVID-19. The most common symptoms were hair loss (56.1%), fatigue (42.9%), dyspnea (33.7%), sleep difficulties (26.5%), memory loss (20.4%), persistent cough (12.2%), myalgia (10.2%), muscle weakness (9.2%), palpitation (8.2%), joint pain (8.2%), and persistent sputum (6.1%). Among them, two symptoms - hair loss and fatigue - often co-occurred with dyspnea or sleep difficulties.

This analysis provides the first indication of the relatively high prevalence of health issues in patients who had recovered from moderate-severe COVID-19 in Ho Chi Minh City, Vietnam. This could help the local health system to detect and manage health issues after moderate-severe COVID-19 in the future.
The role of budget emphasis and participation on budgetary slack for start up industry
Authors: - Anthonius
Published: June 2023

The main background of this research are the problem issues of companies in start up industry. Budgeting has been the crucial factor to be researched, related to efficiency, cost thrift, and performance improvement in start up companies. This quantitative research uses the questionnaire research instrument towards 120 startup company’s managers. The spss method were applied in data processing stage. The testing result shows that budget participation has positive impact toward budgetary slack, while budget emphasis has no impacts whatsoever. The implication of this research’s result can be utilized by startup industries as input related to Budget Participation, budget emphasis and Budgetary Slack so that the appointed budget’s target can be implemented and achieved efficiently. The originality of this research is the test related to budget emphasis, budget participation and budgetary slack in Indonesia’s startup industries.
Pengaruh persepsi kebermanfaatan dan kemudahan penggunaan e-samsat terhadap pembayaran pajak kendaraan bermotor di Kabupaten Bekasi
Authors: Septiani Jati , Siringoringo Whereson
Published: June 2023

The e-samsat application is an improvement from Bapenda service system in serving motor vehicle tax payments by Motor Vehicle Taxpayers. This study aims to determine the effect of taxpayers' perceptions of the usefulness and convenience of the e-samsat application as a factor that influences taxpayers in using the e-samsat application. This research use a quantitative method with the population Motor Vehicle Taxpayers who are registered in Samsat Bekasi Regency. The research results that taxpayers' perceptions of the usefulness of the e-samsat application have a positive effect on the use of e-samsat in motor vehicle tax payments, and taxpayers' perceptions of the ease of use of e-samsat applications have a positive effect on the use of e-samsat in Bekasi Regency.
Analisis determinan harga saham perusahaan perbankan di Bursa Efek Indonesia
Authors: Boru Hotang Keri , Taufik Eindy , . Ivander
Published: June 2023

The purpose of this research is to examine whether audit opinion, firm size dan public accounting firm have an effect on stock prices. The population of this research is banking companies listed on the Indonesia Stock Exchange in 2016-2020. The sample selection wa carried out using a puporsive sampling of banking companies listed on the Indonesia Stock Exchange. This research used IBM SPSS software version 25. The analytical tools used in this test are classical assumption test and multiple regression. The result showed that audit opinion and firm size had a significant effect on stock prices. Yet, the public accounting firm has no significant effect on stock prices.
Indonesia’s tax revenue: The effect of inflation rate and economic growth
Authors: Hamdani Deni , . Ridwan
Published: June 2023

In order to ascertain and analyze the impact of Indonesia's inflation rate and economic growth on tax collections, between 2012 and 2020, the Ministry of Finance's Directorate General of Taxes performed research. Data collection approaches were acquired through literature and documentation for this research, which is categorized as descriptive and verificative research with a quantitative approach. The sampling method employs non-probability sampling, which creates a saturated sample by taking samples from the entire population. Multiple linear regression analysis is used to analyze data, while the t-test and f-test are used to assess hypotheses. The results show that inflation rate has no significant positive effect on tax revenue. Besides, economic growth has no significant negative effect on tax revenue. It is hoped that government will pay attention for tax revenue every year.
Gambaran Pengetahuan Ibu Hamil tentang Deteksi Dini Tanda Bahaya Kehamilan di Puskesmas Tambelan Sampit
Authors: Nisma Nisma , Hidayah Nurul , Jamil Nurul
Published: June 2023

ABSTRACT The morbidity and mortality that occurs in pregnant women is due to a lack of knowledge regarding the causes, effects and management of complications that occur during pregnancy. Lack of knowledge of pregnant women causes mothers to be unable to carry out early detection because they do not recognize the danger signs of pregnancy so that complications of pregnancy are often handled late. This study aims to identify mother's knowledge about early detection of danger signs of pregnancy at the Tambelan Sampit Health Center. This type of research is a type of descriptive research. Sampling in this study using total sampling. Research respondents were pregnant women who checked their pregnancies at the Tambelan Sampit Health Center. The research instrument was a questionnaire regarding the knowledge of pregnant women regarding the detection of danger signs of pregnancy. The results of the study showed that the majority of respondents had sufficient knowledge of 17 respondents (33.3%), had good knowledge of 7 respondents (23.3%) and a small portion had less knowledge of 6 respondents (20%). The conclusion of this study is that the majority of mothers who check their pregnancies at the Tambelan Sampit Health Center have sufficient knowledge about early detection of danger signs of pregnancy.  Keywords: Knowledge, Pregnancy, Early Detection  ABSTRAK Mordibitas dan mortalitas yang terjadi pada ibu hamil disebabkan masih kurangnya pengetahuan mengenai sebab, akibat dan penanggulangan komplikasi yang terjadi selama kehamilan. Kurangnya pengetahuan ibu hamil menyebabkan ibu tidak dapat melakukan deteksi dini karena tidak mengenali tanda bahaya kehamilan sehingga seringkali penanganan komplikasi kehamilan terlambat tertangani. Penelitian ini bertujuan mengidentifikasi pengetahuan ibu tentang deteksi dini tanda bahaya kehamilan  di Puskesmas Tambelan Sampit. enis penelitian ini merupakan jenis penelitian deskriptif. Pengembilan sampel dalam penelitian ini menggunakan total sampling. Responden penelitian adalah ibu hamil yang memeriksakan kehamilan di Puskesmas Tambelan Sampit. Instrument penelitian berupa kuesioner tetang pengetahuan ibu hamil tetang deteksi tanda bahanya kehamilan. Hasil penelitian menujukan responden Sebagian besar berpengetahuan cukup sebanyak 17 responden (33,3%),berpengetahuan baik sebanyak 7 responden (23,3%) dan sebagian kecil berpengetahuan kurang sebanyak 6 responden (20%).Simpulan penelitian ini ialah bebagian besar ibu yang memeriksakan kehamilan di Puskesmas Tambelan Sampit memiliki pengetahuan cukup tetang deteksi dini tanda bahaya kehamilan.Kata Kunci: Pengetahuan, Kehamilan, Deteksi Dini
Identificationof Y Ang Factor Affects Level of Parental Anxiety Towards Covid 19 Vaccination in Children
Authors: Anggraini Ika Rizki , Aini Nurul
Published: June 2023

ABSTRACT The covid 19 virus has affected children, and adolescents and children now account for more than a fifth of new cases in parts of the world. Vaccination is carried out from all ages, including children over 6 years old. But the challenge is about how parents can vaccinate their children. This is important to do because parents are the key in all decision-making determinants in children. In addition, this study is important to be carried out as an evaluation material in achieving the coverage of the covid 19 immunization target in children.  The purpose of this study was to identify actors who influenced the level of parental anxiety towards covid 19 vaccination in Children. This research method uses descriptive analysis with a cross sectional study approach. The population and sample are parents who have children aged 6-18 years. This study used an accidental sampling method with the number of 30respondents of parents who had children aged 6-18 years. The research sites are located in several elementary schools in the Malang city area that have carried out covid 19 vaccinations. The results of this study showed that the age of <50 years old parents, namely 16 people (53.3%), of which 18 people (60%) did not experience anxiety.  Ibu household (IRT) which was 17 respondents (56.7%) with a mild anxiety level of 5 people, respondents were dominated by those with 2 children, namely 21 people (70%) consisting of 4 people experiencing severe anxiety. Conclusion: The results show that parents who have psychological factors are related to the level of parental anxiety towards covid 19 vaccination in children. Keywords: Covid-19, Anxiety, Parents, Vaccination in Children
ЗНАЧУЩІ КОРЕЛЯТИ ПОЗИТИВНОГО ПСИХІЧНОГО ЗДОРОВ’Я У СТРУКТУРІ ПСИХОЛОГІЧНОГО БЛАГОПОЛУЧЧЯ ОСОБИСТОСТІ
Authors: Паловскі Ю.
Published: June 2023

Стаття містить огляд сутнісних характеристик та структури психологічного благополуччя як базисної складової психічного здоров’я особистості. Обґрунтовано, що психічне здоров’я та психологічне благополуччя співвідносяться між собою як ціле і частина. Емпіричним шляхом виявлено низку значущих корелятів психічного здоров’я у структурі психологічного благополуччя військовослужбовців та медичних працівників.
Influence of polymer solution concentration and media permeability on the residual resistance factor
Authors: Moldabayeva G. Zh , Effendiyev G. M , Imansakipova Z. B , Tuzelbayeva Sh. R , Abbasova S. V
Published: June 2023

Purpose. Determination of the required concentration of polymer solution, providing the maximum well insulation effect, depending on the filtration characteristics of the medium. Methodology. The research was conducted by experimental method using the methodology of planning the experiment. The theory of rational planning was used by varying two variables on five levels. Media permeability and polymer solution concentration were used as variables. Methods of mathematical statistics were used in processing the results. Findings. Experimental studies made it possible to build a model expressing the dependence of the residual resistance factor on the permeability of the medium and the concentration of the polymer solution. By further statistical processing the connection between permeability and necessary concentration of polymer solution was obtained. Originality. The experimental studies have substantiated the possibility of evaluating the influence of medium permeability and concentration of polymer solution on the residual resistance factor and determined its dependence on the concentration of polymer solution and medium permeability. The main point of polymer solutions application is justification and choice of the reagent concentration. The concentration should be selected in such a way that it provides the maximum value of the residual resistance factor and the viscosity of the solution necessary to level out permeability heterogeneity of the environment to some extent. To achieve this, a relationship has been obtained that allows determining the concentration of the polymer solution at a given permeability of the environment that provides the maximum residual resistance factor. Practical value. The conducted experimental studies allow developing the ideas about the regularities of water manifestations. The results of the research allow selecting purposefully both the formulations of composite systems and the technology of their application to improve the efficiency of oil production and to limit water inflows in specific geological and physical conditions.
Experimental research on muffle furnace dynamic properties
Authors: Horbiychuk M. I , Lazoriv N. T , Kohutyk M. I , Manuliak I. Z
Published: June 2023

Purpose. To develop the method and software for building empirical models of muffle furnaces with two heating elements. Methodology. The method for processing experimental data according to the improved algorithm, which is based on the areas method, is used for constructing empirical models of the muffle furnace. Experimental research of the muffle furnace dynamic is conducted using the following methodology. The muffle furnace is cooled to the room temperature and then the lower heater is turned on and temperatures at the furnace output are fixed using the experimental two-channel temperature controller MIC-344. The second cycle of the experimental research began by cooling the furnace to the room temperature with turning on the upper heater. Observations based on the results of the experiment are conducted until the temperature is stabilized at the two outputs of the muffle furnace. Archiving of temperature trends is carried out using the RS485/Ethernet interface and the SmartReview software (a product of private limited company “Microl”). Findings. The developed software can be used for iteratively selection of the optimal empirical model based on the criterion of the root mean square deviation of the calculated and experimental data. The calculating method for the acceleration curve based on the transfer functions coefficients with a variable discreteness step is improved in order to compare the experimental data with the empirical modeling results. Twelve empirical models were tested for four signal transmission channels in the process of experimental research. It has been established that only four of them are stable (have left-hand roots of the characteristic equation). Among the selected sustainable models, the model with the numerator polynomial equaled to two and denominator polynomial equaled to three has been established as the best model (according to the established criterion). A structural diagram is created based on the synthesized empirical model of the muffle furnace, which includes four transfer functions and cross connections. Originality. For the first time, the empirical model of a muffle furnace with two electrical energy sources has been developed, which describes its dynamic properties as the object of automatic control with high accuracy, which enables to reveal the presence of internal cross-connections, that significantly complicate the controlling process of the object. Practical value. The created empirical models with two electric sources (tens) can be used for the synthesis of the high-precision automatic temperature control system of the muffle furnace.
PENGEMBANGAN ALAT EVALUASI KOGNITIF BERBASIS ISPRING SUITE 9 PADA MATERI LARUTAN PENYANGGA DI SMA NEGERI 9 KOTA BENGKULU
Authors: Kiki Ramadhanti Pratiwi , Alwi Wiwit , Solikhin Febrian
Published: June 2023

This research was a research and development to produce a cognitive evaluation tool based on iSpring Suite 9. The purpose of this study was to determine the feasibility of items and a cognitive evaluation tool based on iSpring Suite 9 on buffer solution material in terms of the assessment of material experts and media experts, knowing the validity empirical and reliability of the items on the cognitive evaluation tool based on iSpring Suite 9 on buffer solution material, and knowing the results of implementing the cognitive evaluation tool based on iSpring Suite 9 on buffer solution material in class XI MIPA 1 SMA Negeri 9 Bengkulu City. The development model used was the ADDIE development model which consists analysis, design, development, implementation, and evaluation. This research was conducted at SMA Negeri 9 Bengkulu City with the research subjects being 10 students of class XI MIPA 1 for small-scale trials and 30 students of class XI MIPA 1 for field testing. The results obtained (1) the feasibility level of cognitive evaluation tools on the material aspect was 94.25% and from the media aspect was 90.83% or in the very feasible category, (2) based on the results of empirical validation obtained 20 valid items, (3) based on the reliability test obtained a coefficient of 0.951 which was included in the very high category, (4) based on the results of the implementation of the cognitive evaluation tool applied in the classroom, it was proven that it can be used to measure student learning outcomes with a mastery percentage of 83.3%.
UJI AKTIVITAS ANTIKOLESTEROL EKSTRAK BATANG Uncaria cordata (Lour.) Merr. TERHADAP KADAR KOLESTEROL TOTAL MENCIT JANTAN (Mus musculus)
Authors: Indriyani Rika , Dewi Handayani  , Nurhamidah Nurhamidah , Sundaryono Agus
Published: June 2023

The aim of this study was to determine the anticholesterol activity of the stem extract of Uncaria cordata (Lour.) Merr. on total cholesterol levels in male mice (Mus musculus). Before carrying out the anticholesterol activity test, phytochemical tests were carried out to determine the content of secondary metabolites in Uncaria cordata stems. Furthermore, the anticholesterol activity test was carried out in vivo using male mice, male mice were induced by high-fat diet (PTL) in the form of beef brain, quail egg yolk and propylthiouracil to increase cholesterol levels in mice until they experienced hypercholesterolemia, then mice were induced by plant extracts to see anticholesterol activity. Cholesterol levels were measured using a glucometer (Autocheck). Phytochemical test results showed the content of secondary metabolites in the form of flavonoids, saponins, alkaloids, tannins and terpenoids. The results of the anti-cholesterol activity test showed that there was anti-cholesterol activity as indicated by a decrease in cholesterol levels in mice with hypercholesterolemia. A decrease of 11.45% at a dose of 5.3 mg/30grW; 12.89% at a dose of 10.2mg/30grW and 21.05% at a dose of 21.2mg/30grW.

Key words : Anticholesterol, Uncaria cordata (Lour.) Merr., Hypercholesterolemia
Good practices for the development of budget impact models at regional level
Authors: Marcellusi Andrea , Ragonese Angela , Marinozzi Andrea , Bortolami Alberto , Mucherino Sara , Moreno Carolina , Antenori Amalia , Ferrario Matteo , Simonelli Claudia , Zanuzzi Matteo , Cicoira Marco , Lasala Ruggero , Russoniello Francesco , Attanasio Francesco , Donati Caterina , Roni Chiara , Gemmi Fabrizio , Mennini Francesco Saverio , Russo Pierluigi , Scroccaro Giovanna , Canonico PierLuigi
Published: June 2023

Introduction: The present work aims to discuss the current scenario of procedures and regulations regarding budget impact analysis/models (BIA/BIM) at regional level in Italy and to provide a standardized approach and detailed recommendations for developing these analyses. Method: A systematic review of the literature was conducted in order to collect existing guidelines or specific regional procedures for budget impact analysis in Italy. All the records were analysed in qualitative terms according to a pre-specified analytical framework, based on the ISPOR BIA guidelines. At the end of the analysis, a consensus questionnaire was developed to establish agreed approaches and to provide possible solutions to any critical issues. A list of 39 statements was developed. The survey was distributed to 69 experts who rated their level of agreement with each statement on a 5-point Likert scale. Consensus was predefined as more than 66% of the panel agreeing/disagreeing with any given statement. Results: Sisty-nine experts answered the questionnaire; a total of 30/39 statements achieved consensus. There was agreement on most of the statements. Time horizon to consider and costs were the issues on which no agreement was found. The results allowed the working group to define a list of good practices. Conclusion: While the structure and development of BIM are now well-known and well-applied at national level, there remains a great diversity of management of BIM tools at regional level. Consensus was reached among participating experts, as to the main characteristics, determinants and features of regional BIA/BIM in the perspective of the Italian payer.
Óbito infantil por anemia nutricional no Brasil entre os anos de 2008 e 2020: um estudo epidemiológico
Authors: Cajaiba Ryan Ferreira , Gomes Victor Alexandre Santos , Costa Juliane Nascimento , Silveira Mayara da Cruz , Damasceno Pollyanna Ribeiro , Trindade Thaís Pereira , Ferreira Mariane Santos
Published: June 2023

A anemia caracteriza-se pela baixa taxa de hemoglobina no sangue. Em crianças, esta patologia constitui grave problema, uma vez que é prejudicial para o desenvolvimento do organismo infantil e por vezes ocasionando o óbito. Desse modo, este trabalho tem por objetivo descrever o perfil epidemiológico de óbitos infantis por anemias nutricionais entre regiões brasileiras no período de 2008 e 2020. Para tanto, trata-se de um estudo ecológico, de caráter transversal, retrospectivo, descritivo e quantitativo, a partir de dados secundários do Departamento de Informática do Sistema Único de Saúde, seguindo as variáveis: região, período, local de ocorrência, faixa etária, sexo, etnia/raça, idade da mãe, escolaridade, duração da gestação, tipo de gravidez, tipo de parto e peso ao nascer. No qual a pesquisa identificou o nordeste com maior taxa de óbitos (53%), seguido da região Norte (27%), Sudeste (9,2%), Centro-Oeste (5,8%) e Sul (3,3%), representando uma grande disparidade entre as regiões. Os fatores que provocam tal disparidade estão intimamente ligados às condições socioeconômicas que os indivíduos se encontram. Assim, é fundamental que se combata as desigualdades socioeconômicas, promovendo o acesso igualitário aos serviços de saúde para o combate à mortalidade infantil por meio do diagnóstico precoce. Tornando, assim, a mortalidade infantil por anemia nutricional menos incidente.
Sífilis adquirida: uma investigação epidemiológica
Authors: Couto Nivia Colares , Freitas Thiago Colares , Ataide Pedro Paulo Oliveira
Published: June 2023

A sífilis adquirida é uma das infecções sexualmente transmissíveis mais preocupantes no Brasil, pois exibe vários estágios podendo causar de lesões teciduais a demência e dano vascular nem sempre apresenta manifestações clínica mesmo assintomática uma pessoa pode transmitir e disseminar a infecção tornando-a uma epidemiologia de difícil controle. O objetivo dessa pesquisa é analisar a distribuição espacial dos indivíduos acometidos por sífilis adquirida na região Norte do Brasil no período de 2019- 2022, fomentando as estratégias de ações e controle da doença considerando os interferentes de acesso ao tratamento. A pesquisa realizada é de natureza descritiva, retrospectiva, clinica epidemiológica através de um estudo das análises do sistema de informações de agravo de notificação (Sinan Net). Os resultados indicam que no período de análise foram notificados 39.884 casos de sífilis somente na região Norte do Brasil onde o estado do Amazonas (37%), Pará (25%) e Rondônia (12%) lideram a lista de notificações seguidos dos estados com menores notificações, Tocantins (10%), Acre (6%), Amapá (5%) e Roraima (5%). O gênero masculino continua sendo o mais afetado já a faixa etária 20 a 29 para ambos os gêneros é a mais recorrente há mais de uma década sugerindo que sejam traçados planos de educação sexual, proteção, prevenção e tratamentos mais eficientes para esse público.
Impact of Diﬀerent Physiotherapy Programs on Balance and Gait in Patients with Cerebral Infarction
Authors: Smailytė Paula , Lendraitienė Eglė , Žemaitienė Ieva
Published: June 2023

Background. An ischemic stroke has long-term adverse effects on the physical, emotional, and social well-being of the stroke survivor, so it is very important to help the patient regain independence and lost functions as quickly and efficiently as possible. Dynamic Neuromuscular Stability exercises can restore movement patterns in these patients by simulating central nervous system.

The aim. To compare the effects of conventional physiotherapy and dynamic neuromuscular stabilization integrated into conventional physiotherapy on balance and gait in patients with cerebral infarction.

Methods. The study included 30 subjects who survived an ischemic stroke. Subjects were randomly divided into two groups of 15 people in each for different interventions: Conventional physiotherapy and Conventional physiotherapy combined with integrated dynamic neuromuscular stabilization. Subjects were examined at a baseline and after the interventions. Patients’ balance was assessed using the Biodex platform, which was chosen to evaluate fall risk, trunk stability, stability limits and sensory organization. Gait parameters (step length and step width) of the affected and unaffected leg were assessed using the Zebris FDM-T treadmill.

Results. Both conventional physiotherapy and physiotherapy with integrated dynamic neuromuscular stabilization improved gait parameters of the patients, as well as improving the results of the tests of fall risk, postural stability, stability limits, and the results of the sensory integration test when testing with eyes open on a stable surface (p<0.05). However, the results of the sensory integration tests while standing on a stable surface with eyes closed (p<0.001), with eyes open on an unstable surface (p<0.001) and with eyes closed on an unstable surface (p=0.021) improved only post physiotherapy with integrated dynamic neuromuscular stabilization.

Conclusions. Both conventional physiotherapy and physiotherapy with integrated dynamic neuromuscular stabilization significantly improved gait and balance parameters in stroke patients.

 Keywords: Dynamic neuromuscular stabilization, stroke, balance, trunk stability, gait.
Верификация расчетных методик на основе экспериментальных исследований фрагментов железобетонных блоков
Authors: АМБАРЦУМЯН Сергей Александрович , МАНУКЯН Артур Ванушевич , МКРТЫЧЕВ Олег Вартанович , АНДРЕЕВ Михаил Иванович
Published: June 2023

Решение вопросов увеличения объемов жилищного строительства и доступности жилья для населения возможно только при повышении скорости строительства. Строительство многоэтажных зданий из крупномодульных железобетонных блоков - один из способов решения этой задачи. Приведены результаты исследований в части расчетного сопровождения испытаний на сдвиг фрагментов крупномодульных железобетонных блоков с доведением их до разрушения. Разработанные с учетом полученных материалов расчетные методики и модели объемных блоков позволяют учесть действительную жесткость (податливость) узлов, нелинейный характер деформирования несущих элементов и реальную диаграмму работы материала. Такие подходы дают возможность применять их в расчете многоэтажных зданий из объемных железобетонных блоков и проектировать оптимальные конструкции с учетом имеющихся резервов несущей способности.
Smart Governance Policy: An Overview of The Local Government Performance
Authors: Sarofah Riska
Published: June 2023

One of the challenges in digital transformation is building a comprehensive digital ecosystem. This article aims to analyze smart governance policies in improving government efficiency in Indonesia. The number of applications cannot be used as a benchmark in determining the performance of governance implementation. This article uses a qualitative research method with the approach of a systematic literature review. The results of the study found that smart governance policies are oriented to 4 indicators, including community participation, effective governance, public and social services, and political perspectives and strategies. This policy will be successful if implemented collaboratively and integratively. The novelty of the research is in the exploration of policies in local government because until now smart governance policies have not been fully implemented, especially in regions that do not have a clear vision strategy. This research also develops various smart governance policy challenges including digital technology infrastructure, organization, policy reform, and collaborative work development.
Kualitas Terjemahan Idiom Dan Technical Terminolgy Dengan Google Translate (Studi Kasus: Terjemahan Mahasiswa)
Authors: Afriani Afriani
Published: June 2023

Tujuan dari penelitian ini adalah untuk menganalisis kualitas terjemahan idiom dan terminologi khusus yang dilakukan oleh mahasiswa dengan menggunakan teknologi penerjemahan berupa ‘Google Translate’ (GT). Data diperoleh dengan menggunakan dua metode. Pertama, satu set instrumen yang berisi teks sumber (TSu)dalam bahasa Inggris. Siswa diminta untuk menerjemahkan ke dalam bahasa Indonesia sebagai bahasa sasaran (BSa). Kedua, wawancara untuk mendapatkan informasi tentang pengalaman dan pendapat mahasiswa tentang penggunaan GT. Terjemahan yang dihasilkan untuk idiom dan terminologi khusus tidak berkualitas karena dua sebab. Pertama, GT tidak dapat menerjemahkan unsur budaya karena masih dibutuhkan penerjemah manusia dalam proses penerjemahan. Kedua, mahasiswa kurang memiliki pengetahuan tentang bagaimana menggunakan GT dengan baik, sehingga pendidikan terkait GT menjadi penting bagi para penerjemah. Rekomendasi dari penelitian ini adalah mahasiswa atau penerjemah terlebih dahulu harus mengidentifikasi idiom dan terminologi khusus TSu sebelum menerjemahkan menggunakan GT. Kedua, mahasiswa atau penerjemah harus mengetahui konteks TSu sebelum menerjemahkan menggunakan GT. Terakhir, teks sasaran yang dihasilkan dengan GT harus dibaca ulang sehingga terasa wajar dan berterima di dalam budaya pembaca BSa.
The Potential Antibacterial Effect of Papaya Leaf Extract (Carica papaya L) and Miana Leaf Extract (Coleus scutellarioides L) as Adjuvant Therapy for Rifampicin-Resistant Tuberculosis
Authors: Zaini Wawan Sofwan , Kurniati Nining , Khayan Khayan , Ihsan Bagus Muhammad , Puspita Widyana Lakshmi , Hanif Muhammad Ifham
Published: June 2023

The adhesion of Rifampicin-resistant TB to neutrophils plays an essential role in colonization. Several active compounds in papaya leaf and Miana leaf (Coleus scutellarioides L) are believed to regulate or prevent the formation of bacterial colonies. The purpose of this study was to determine the anti-bacterial effectiveness of extracts of papaya leaf (Carica Papaya L.) and Miana leaf (Coleus scutellarioides L) against bacterial isolates of Rifampicin-Resistant TB strain (RR). This research method is a in vitro laboratory experiment, and extracts of papaya leaf and Miana leaf (50, 25, 12,5%) were tested as anti-bacterial using the M-TB susceptibility test using the Proportion Method. The results showed the anti-bacterial ability of papaya leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 0% so that it was included in the Sensitive category, but at a concentration of 25% the resistance percentage value was 42.86% % and a concentration of 12.5%, the percentage value of resistance is 42.86% so that it is included in the category of resistance (Resistant > 1% and Sensitive < 1%). The anti-bacterial ability of miana leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 5.33%, at a concentration of 25%, with a resistance percentage value of 17.14%, and at a concentration of 12.5%, with a resistance percentage value of 100%, so all are included in the resistant category. The Conclusion 50% papaya leaf extract inhibits the formation of Rifampicin-resistant MTBC-resistant bacterial colonies, allowing its usage as a substitute ingredient in Rifampicin-resistant MTBC-resistant medications.
Dissecting regulatory T cell expansion using polymer microparticles presenting defined ratios of self-antigen and regulatory cues
Authors: Bridgeman Christopher J. , Shah Shrey A. , Oakes Robert S. , Jewell Christopher M.
Published: June 2023

Biomaterials allow for the precision control over the combination and release of cargo needed to engineer cell outcomes. These capabilities are particularly attractive as new candidate therapies to treat autoimmune diseases, conditions where dysfunctional immune cells create pathogenic tissue environments during attack of self-molecules termed self-antigens. Here we extend past studies showing combinations of a small molecule immunomodulator co-delivered with self-antigen induces antigen-specific regulatory T cells. In particular, we sought to elucidate how different ratios of these components loaded in degradable polymer particles shape the antigen presenting cell (APC) -T cell interactions that drive differentiation of T cells toward either inflammatory or regulatory phenotypes. Using rapamycin (rapa) as a modulatory cue and myelin self-peptide (myelin oligodendrocyte glycoprotein- MOG) – self-antigen attacked during multiple sclerosis (MS), we integrate these components into polymer particles over a range of ratios and concentrations without altering the physicochemical properties of the particles. Using primary cell co-cultures, we show that while all ratios of rapa:MOG significantly decreased expression of co-stimulation molecules on dendritic cells (DCs), these levels were insensitive to the specific ratio. During co-culture with primary T cell receptor transgenic T cells, we demonstrate that the ratio of rapa:MOG controls the expansion and differentiation of these cells. In particular, at shorter time points, higher ratios induce regulatory T cells most efficiently, while at longer time points the processes are not sensitive to the specific ratio. We also found corresponding changes in gene expression and inflammatory cytokine secretion during these times. The in vitro results in this study contribute to in vitro regulatory T cell expansion techniques, as well as provide insight into future studies to explore other modulatory effects of rapa such as induction of maintenance or survival cues.
Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes
Authors: Pisani Silvia , Mauri Valeria , Negrello Erika , Mauramati Simone , Alaimo Gianluca , Auricchio Ferdinando , Benazzo Marco , Dorati Rossella , Genta Ida , Conti Bice , Ferretti Virginia Valeria , De Silvestri Annalisa , Pietrabissa Andrea , Marconi Stefania
Published: June 2023

Introduction: The problem of organs’ shortage for transplantation is widely known: different manufacturing techniques such as Solvent casting, Electrospinning and 3D Printing were considered to produce bioartificial scaffolds for tissue engineering purposes and possible transplantation substitutes. The advantages of manufacturing techniques’ combination to develop hybrid scaffolds with increased performing properties was also evaluated.

Methods: Scaffolds were produced using poly-L-lactide-co-caprolactone (PLA-PCL) copolymer and characterized for their morphological, biological, and mechanical features.

Results: Hybrid scaffolds showed the best properties in terms of viability (>100%) and cell adhesion. Furthermore, their mechanical properties were found to be comparable with the reference values for soft tissues (range 1–10 MPa).

Discussion: The created hybrid scaffolds pave the way for the future development of more complex systems capable of supporting, from a morphological, mechanical, and biological standpoint, the physiological needs of the tissues/organs to be transplanted.
The covert symphony: cellular and molecular accomplices in breast cancer metastasis
Authors: Si Hongjiang , Esquivel Madelyn , Mendoza Mendoza Erika , Roarty Kevin
Published: June 2023

Breast cancer has emerged as the most commonly diagnosed cancer and primary cause of cancer-related deaths among women worldwide. Although significant progress has been made in targeting the primary tumor, the effectiveness of systemic treatments to prevent metastasis remains limited. Metastatic disease continues to be the predominant factor leading to fatality in the majority of breast cancer patients. The existence of a prolonged latency period between initial treatment and eventual recurrence in certain patients indicates that tumors can both adapt to and interact with the systemic environment of the host, facilitating and sustaining the progression of the disease. In order to identify potential therapeutic interventions for metastasis, it will be crucial to gain a comprehensive framework surrounding the mechanisms driving the growth, survival, and spread of tumor cells, as well as their interaction with supporting cells of the microenvironment. This review aims to consolidate recent discoveries concerning critical aspects of breast cancer metastasis, encompassing the intricate network of cells, molecules, and physical factors that contribute to metastasis, as well as the molecular mechanisms governing cancer dormancy.
Laboratory automation, informatics, and artificial intelligence: current and future perspectives in clinical microbiology
Authors: Mencacci Antonella , De Socio Giuseppe Vittorio , Pirelli Eleonora , Bondi Paola , Cenci Elio
Published: June 2023

Clinical diagnostic laboratories produce one product—information—and for this to be valuable, the information must be clinically relevant, accurate, and timely. Although diagnostic information can clearly improve patient outcomes and decrease healthcare costs, technological challenges and laboratory workflow practices affect the timeliness and clinical value of diagnostics. This article will examine how prioritizing laboratory practices in a patient-oriented approach can be used to optimize technology advances for improved patient care.
Securing small and medium ports and their supply chain services
Authors: Kyranoudi Pinelopi , Polemi Nineta
Published: June 2023

This paper argues that small and medium sized ports (SMPs) are as important as larger ones in terms of supply chain service (SCS) management and security, as they can become the weakest links for national and European Union (EU) resilience and security. It focuses on explaining key concepts about SMPs, their characteristics (e.g., size, operational field, infrastructure), potential threats (e.g., interception of sensitive information, illegal access, terrorism) and attacks (cyber, cyber-physical), as well as basic security concepts (e.g., attack path, attack vector, risk). Three SCS attack scenarios for SMPs are described based on different types of threats, which could cause catastrophic impacts, even paralyzing an SMP propagated in its SCS. Finally, a risk management methodology for SCSs that can be used by SMPs, named CYSMET, is presented considering their capabilities, needs and constraints.
Levels and clinical significance of the m6A methyltransferase METTL14 in patients with coronary heart disease
Authors: Guo Fengxia , He Mei , Hu Bing , Li Gang
Published: June 2023

Objective

To investigate the association of methyltransferase-like protein 14 (METTL14) expression with coronary heart disease (CHD).

Methods

Three hundred and sixteen patients who attended Henan Provincial People's Hospital between June 2019 and February 2021 with principal symptoms of pain or tightness in the chest and who underwent coronary angiography for definitive diagnosis were enrolled. The uric acid, TG, TC, LDL-C, HDL-C, apolipoprotein A1, free fatty acid, lipoprotein a, homocysteine, CRP, and SAA levels were examined. The levels of METTL14, TNF-α, MCP-1, VCAM-1, ICAM-1, and IL-6 were evaluated by ELISA.

Results

Patients with CHD had significantly higher m6A methyltransferase activity. In addition, the incidence of diabetes and hypertension, as well as the concentrations of TC, CRP, and SAA were higher in CHD patients. Patients with coronary lesion branches also had significantly increased TG, LDL-C, CRP, and SAA levels. TNF-α, MCP-1, VCAM-1, ICAM-1, and IL-6 expression was also markedly increased in the CHD group (P < 0.001) as was the expression of METTL14 (P < 0.001). The METTL14 expression levels also differed significantly in relation to the number of branches with lesions (P < 0.01) and were correlated with SAA, VCAM-1, ICAM-1, IL-6, and the Gensini score. ROC curve analyses of METTL14 in CHD indicated an AUC of 0.881 (0.679, 0.894) with a cut-off value of 342.37, a sensitivity of 77%, and a specificity of 84%. MCP-1, VCAM-1, IL-6, SAA, and METTL14 were found to independently predict CHD risk.

Conclusions

METTL14 levels were found to be positively associated with inflammatory markers and to be an independent predictor of CHD risk.
Physiological and pathological characteristics of vascular endothelial injury in diabetes and the regulatory mechanism of autophagy
Authors: Liu Hanyu , Wang Xueru , Gao Hong , Yang Chan , Xie Chunguang
Published: June 2023

Vascular endothelial injury in diabetes mellitus (DM) is the major cause of vascular disease, which is closely related to the occurrence and development of a series of vascular complications and has a serious negative impact on a patient’s health and quality of life. The primary function of normal vascular endothelium is to function as a barrier function. However, in the presence of DM, glucose and lipid metabolism disorders, insulin resistance, inflammatory reactions, oxidative stress, and other factors cause vascular endothelial injury, leading to vascular endothelial lesions from morphology to function. Recently, numerous studies have found that autophagy plays a vital role in regulating the progression of vascular endothelial injury. Therefore, this article compares the morphology and function of normal and diabetic vascular endothelium and focuses on the current regulatory mechanisms and the important role of autophagy in diabetic vascular endothelial injury caused by different signal pathways. We aim to provide some references for future research on the mechanism of vascular endothelial injury in DM, investigate autophagy’s protective or injurious effect, and study potential drugs using autophagy as a target.
Decreased IGF1R attenuates senescence and improves function in pancreatic β-cells
Authors: Iwasaki Kanako , Lalani Benjamin , Kahng Jiho , Carapeto Priscila , Sanjines Stephanie , Hela Francesko , Abarca Cristian , Tsuji Tadataka , Darcy Justin , Bartke Andrzej , Tseng Yu-Hua , Kulkarni Rohit N. , Aguayo-Mazzucato Cristina
Published: June 2023

Introduction

The enhanced β-cell senescence that accompanies insulin resistance and aging contributes to cellular dysfunction and loss of transcriptional identity leading to type 2 diabetes (T2D). While senescence is among the 12 recognized hallmarks of aging, its relation to other hallmarks including altered nutrient sensing (insulin/IGF1 pathway) in β-cells is not fully understood. We previously reported that an increased expression of IGF1R in mouse and human β-cells is a marker of older β-cells; however, its contribution to age-related dysfunction and cellular senescence remains to be determined.

Methods

In this study, we explored the direct role of IGF1R in β-cell function and senescence using two independent mouse models with decreased IGF1/IGF1R signaling: a) Ames Dwarf mice (Dwarf +/+), which lack growth hormone and therefore have reduced circulating levels of IGF1, and b) inducible β-cell-specific IGF1R knockdown (βIgf1rKD) mice.

Results

Compared to Dwarf+/- mice, Dwarf+/+ mice had lower body and pancreas weight, lower circulating IGF1 and insulin levels, and lower IGF1R and p21Cip1 protein expression in β-cells, suggesting the suppression of senescence. Adult βIgf1rKD mice showed improved glucose clearance and glucose-induced insulin secretion, accompanied by decreased p21Cip1 protein expression in β-cells. RNA-Seq of islets isolated from these βIgf1rKD mice revealed the restoration of three signaling pathways known to be downregulated by aging: sulfide oxidation, autophagy, and mTOR signaling. Additionally, deletion of IGF1R in mouse β-cells increased transcription of genes important for maintaining β-cell identity and function, such as Mafa, Nkx6.1, and Kcnj11, while decreasing senescence-related genes, such as Cdkn2a, Il1b, and Serpine 1. Decreased senescence and improved insulin-secretory function of β-cells were also evident when the βIgf1rKD mice were fed a high-fat diet (HFD; 60% kcal from fat, for 5 weeks).

Discussion

These results suggest that IGF1R signaling plays a causal role in aging-induced β-cell dysfunction. Our data also demonstrate a relationship between decreased IGF1R signaling and suppressed cellular senescence in pancreatic β-cells. Future studies can further our understanding of the interaction between senescence and aging, developing interventions that restore β-cell function and identity, therefore preventing the progression to T2D.
Electroacupuncture treatment ameliorates metabolic disorders in obese ZDF rats by regulating liver energy metabolism and gut microbiota
Authors: Ding Lei , Teng Rufeng , Zhu Yifei , Liu Fengming , Wu Lili , Qin Lingling , Wu Xi , Liu Tonghua
Published: June 2023

Metabolic disorders represent a major therapeutic challenge to public health worldwide due to their dramatically increasing prevalence. Acupuncture is widely used as adjuvant therapy for multiple metabolic diseases. However, detailed biological interpretation of the acupuncture stimulations is still limited. The gut and the liver are intrinsically connected and related to metabolic function. Microbial metabolites might affect the gut-liver axis through multiple mechanisms. Liver metabolomics and 16S rRNA sequencing were used to explore the specific mechanism of electroacupuncture in treating ZDF rats in this study. Electroacupuncture effectively improved glycolipid metabolism disorders of the ZDF rats. Histopathology confirmed that electroacupuncture improved diffuse hepatic steatosis and hepatocyte vacuolation, and promoted glycogen accumulation in the liver. The treatment significantly improved microbial diversity and richness and upregulated beneficial bacteria that maintain intestinal epithelial homeostasis and decreased bacteria with detrimental metabolic features on host metabolism. Liver metabolomics showed that the main effects of electroacupuncture include reducing the carbon flow and intermediate products in the TCA cycle, regulating the metabolism of various amino acids, and inhibiting hepatic glucose output and de novo lipogenesis. The gut-liver axis correlation analysis showed a strong correlation between the liver metabolites and the gut microbiota, especially allantoin and Adlercreutzia. Electroacupuncture treatment can improve abnormal energy metabolism by reducing oxidative stress, ectopic fat deposition, and altering metabolic fluxes. Our results will help us to further understand the specific mechanism of electroacupuncture in the treatment of metabolic diseases.