bluuebunny/crossref_metadata_2025 huggingface dataset:
The GATA-Type Transcriptional Factor Are1 Modulates the Expression of Extracellular Proteases and Cellulases in Trichoderma reesei
Authors: Qian Yuanchao , Sun Yu , Zhong Lixia , Sun Ningning , Sheng Yifan , Qu Yinbo , Zhong Yaohua
Published: August 2019

Trichoderma reesei is a biotechnologically important filamentous fungus with the remarkable ability to secrete large amounts of enzymes, whose production is strongly affected by both the carbon and nitrogen sources. While the carbon metabolism regulators are extensively studied, the regulation of enzyme production by the nitrogen metabolism regulators is still poorly understood. In this study, the GATA transcription factor Are1, which is an orthologue of the Aspergillus global nitrogen regulator AREA, was identified and characterized for its functions in regulation of both protease and cellulase production in T. reesei. Deletion of the are1 gene abolished the capability to secrete proteases, and complementation of the are1 gene rescued the ability to produce proteases. Quantitative RT-PCR analysis revealed that the transcripts of protease genes apw1 and apw2 were also significantly reduced in the Δare1 strain when grown in the medium with peptone as the nitrogen source. In addition, deletion of are1 resulted in decreased cellulase production in the presence of (NH4)2SO4. Consistent with the reduction of cellulase production, the transcription levels of the major cellulase genes, including cbh1, cbh2, egl1, and egl2, were dramatically decreased in Δare1. Sequence analysis showed that all promoter regions of the tested protease and cellulase genes contain the consensus GATA elements. However, the expression levels of the major cellulase transcription activator Xyr1 and the repressor Cre1 had no significant difference between Δare1 and the parental strain QM9414, indicating that the regulatory mechanism deserves further investigation. Taken together, these results demonstrate the important role of Are1 in the regulation of protease and cellulase production in T. reesei, although these processes depend on the kind of nitrogen sources. The findings in this study contribute to the understanding of the regulation network of carbon and nitrogen sources in filamentous fungi.
VceC Mediated IRE1 Pathway and Inhibited CHOP-induced Apoptosis to Support Brucella Replication in Goat Trophoblast Cells
Authors: Zhi Feijie , Zhou Dong , Bai Furong , Li Junmei , Xiang Caixia , Zhang Guangdong , Jin Yaping , Wang Aihua
Published: August 2019

The effectors of the type IV secretion system (T4SS) of bacteria play important roles in mediating bacterial intracellular proliferation and manipulating host-related pathway responses to bacterial infection. Brucella Spp. inhibit the apoptosis of host cells to benefit their own intracellular proliferation. However, the underlying mechanisms between T4SS effectors and Brucella-inhibited apoptosis in goat trophoblast cells remain unclear. Here, based on Brucella suis vaccine strain 2, the VceC was deleted by allelic exchange. We show that ΔVceC was able to infect and proliferate to high titers in goat trophoblast cells (GTCs) and increase C/EBP-homologous protein (CHOP)-mediated apoptosis. GRP78 expression decreased upon ΔVceC infection. In addition, we discovered that the inositolrequiring enzyme 1 (IRE1) pathway was inhibited in this process. Changing endoplasmic reticulum (ER) stress affected Brucella intracellular replication in GTCs. The replication of ΔVceC was more sensitive under the different ERstress conditions in the GTC line after treatment with ER stress inhibitors 4 phenyl butyric acid (4-PBA) or ER stress activator Tm. Together, our findings show that VceC has a protective effect on the intracellular persistence of Brucella infection, and inhibits ER stress-induced apoptosis in the CHOP pathway. The present work provides new insights for understanding the mechanism of VceC in the establishment of chronic Brucella infection.
The Hen or the Egg: Impaired Alveolar Oxygen Diffusion and Acute High-altitude Illness?
Authors: Mairbäurl Heimo , Dehnert Christoph , Macholz Franziska , Dankl Daniel , Sareban Mahdi , Berger Marc M.
Published: August 2019

Individuals ascending rapidly to altitudes >2500 m may develop symptoms of acute mountain sickness (AMS) within a few hours of arrival and/or high-altitude pulmonary edema (HAPE), which occurs typically during the first three days after reaching altitudes above 3000–3500 m. Both diseases have distinct pathologies, but both present with a pronounced decrease in oxygen saturation of hemoglobin in arterial blood (SO2). This raises the question of mechanisms impairing the diffusion of oxygen (O2) across the alveolar wall and whether the higher degree of hypoxemia is in causal relationship with developing the respective symptoms. In an attempt to answer these questions this article will review factors affecting alveolar gas diffusion, such as alveolar ventilation, the alveolar-to-arterial O2-gradient, and balance between filtration of fluid into the alveolar space and its clearance, and relate them to the respective disease. The resultant analysis reveals that in both AMS and HAPE the main pathophysiologic mechanisms are activated before aggravated decrease in SO2 occurs, indicating that impaired alveolar epithelial function and the resultant diffusion limitation for oxygen may rather be a consequence, not the primary cause, of these altitude-related illnesses.
Screening Pathways through China, the Asia Pacific Region, the World
Authors: Wiley Veronica , Webster Dianne , Loeber Gerard
Published: August 2019

The International Society for Neonatal Screening (ISNS) has met regularly at both international meetings and those of the various chapters [...]
In Vivo Effects of A Pro-PO System Inhibitor on the Phagocytosis of Xenorhabdus Nematophila in Galleria Mellonella Larvae
Authors: De Lerma Barbaro Andrea , Gariboldi Marzia B. , Mastore Maristella , Brivio Maurizio F. , Giovannardi Stefano
Published: August 2019

Xenorhabdus nematophila is a Gram-negative bacterium symbiont of the entomopathogen nematode Steinernema carpocapsae whose immunosuppressive properties over host’s immune response have been thoroughly investigated. In particular, live X. nematophila actively impairs phagocytosis in host’s hemocytes through the secretion of inhibitors of eicosanoids synthesis. In this article we have investigated the cell surface structural features of X. nematophila responsible for the elusion from phagocytosis. To this end we have studied the uptake of heat-killed (hk), fluorescein isothiocyanate (FITC)-labeled X. nematophila by phagocytes from both a host insect and a mammalian species. In vitro dead X. nematophila passively resists engulfment by insect hemocytes without impairing the phagocytosis machinery whereas, unexpectedly, in vivo a significant phagocytosis of dead X. nematophila was observed. X. nematophila in vivo phagocytosis was increased by the co-injection of the specific inhibitor of pro-phenoloxidase (PO) system phenylthiourea (PTU), even if these effects were not observed in in vitro tests. Furthermore, biochemical modifications of X. nematophila cell wall implement in vivo phagocytosis, suggesting that this bacterium avoid phagocytosis because the ligand of phagocytic receptors is somehow buried or disguised in the cell wall. Finally, dead X. nematophila escapes engulfment even by human phagocytes suggesting that X. nematophila could be a useful model to investigate escape from phagocytosis by mammalian macrophages.
Investigation of Relative Development and Reproductivity Fitness Cost in Three Insecticide-Resistant Strains of Aedes aegypti from Thailand
Authors: Saingamsook Jassada , Yanola Jintana , Lumjuan Nongkran , Walton Catherine , Somboon Pradya
Published: August 2019

Knockdown resistance (kdr) and detoxification enzymes are major resistance mechanisms in insecticide-resistant Aedes aegypti throughout the world. Persistence of the resistance phenotype is associated with high fitness of resistance alleles in the absence of insecticide pressure. This study determined the relative fitness cost of three insecticide-resistant strains of Aedes aegypti—PMD, PMD-R, and UPK-R—and a hybrid under similar laboratory conditions in the absence of insecticide. The PMD strain is resistant to DDT with no kdr alleles; the PMD-R is resistant to DDT and permethrin with 1534C homozygous kdr alleles; and UPK-R is resistant to DDT, permethrin, and deltamethrin with 989P + 1016G homozygous alleles. The DDT-resistant PMD strain had the highest fitness compared with the two DDT/pyrethroid-resistant strains (PMD-R and UPK-R) and hybrid. Consistent fitness costs were observed in the DDT/pyrethroid-resistant strains and hybrid, including shorter wing length, reduced egg hatchability, shorter female lifespan, and shorter viability of eggs after storage, whereas no effect was observed on blood feeding rate. In addition, reduced egg production was observed in the PMD-R strain and prolonged developmental time was seen in the UPK-R strain. The corresponding hybrid that is heterozygous for kdr alleles was fitter than either of the homozygous mutant genotypes. This is in accordance with the high frequency of heterozygous genotypes observed in natural populations of Ae. aegypti in Chiang Mai city.
Efficacy and Efficiency of Nutritional Support Teams
Authors: Reber Emilie , Strahm Rachel , Bally Lia , Schuetz Philipp , Stanga Zeno
Published: August 2019

Malnutrition is frequent in patients during a hospital admission and may further worsen during the hospital stay without appropriate nutritional support. Malnutrition causes greater complication rates, morbidity, and mortality rates, which increases the length of hospital stay and prolongs rehabilitation. Early recognition of individual nutritional risk and timely initiation of a tailored nutritional therapy are crucial. Recent evidence from large-scale trials suggests that efficient nutritional management not only improves the nutritional status, but also prevents negative clinical outcomes and increases patients’ quality of life. Multifaceted clinical knowledge is required to ensure optimal nutritional support, according to a patient’s individual situation and to avoid potential complications. Furthermore, clear definition of responsibilities and structuring of patient, and work processes are indispensable. Interdisciplinary and multiprofessional nutritional support teams have been built up to ensure and improve the quality and safety of nutritional treatments. These teams continuously check and optimize the quality of procedures in the core areas of nutritional management by implementing nutritional screening processes using a validated tool, nutritional status assessment, an adequate nutritional care plan development, prompt and targeted nutritional treatment delivery, and provision of accurate monitoring to oversee all aspects of care, from catering to artificial nutrition. The foundation of any nutritional care plan is the identification of patients at risk. The aim of this narrative review is to provide an overview about composition, tasks, and challenges of nutritional support teams, and to discuss the current evidence regarding their efficiency and efficacy in terms of clinical outcome and cost effectiveness.
Research and Development in Carbon Fibers and Advanced High-Performance Composites Supply Chain in Europe: A Roadmap for Challenges and the Industrial Uptake
Authors: Koumoulos Elias , Trompeta Aikaterini-Flora , Santos Raquel-Miriam , Martins Marta , Santos Cláudio , Iglesias Vanessa , Böhm Robert , Gong Guan , Chiminelli Agustin , Verpoest Ignaas , Kiekens Paul , Charitidis Costas
Published: August 2019

Structural materials, typically based on metal, have been gradually substituted by high-performance composites based on carbon fibers, embedded in a polymer matrix, due to their potential to provide lighter, stronger, and more durable solutions. In the last decades, the composites industry has witnessed a sustained growth, especially due to diffusion of these materials in key markets, such as the construction, wind energy, aeronautics, and automobile sectors. Carbon fibers are, by far, the most widely used fiber in high-performance applications. This important technology has huge potential for the future and it is expected to have a significant impact in the manufacturing industry within Europe and, therefore, coordination and strategic roadmapping actions are required. To lead a further drive to develop the potential of composites into new sectors, it is important to establish strategic roadmapping actions, including the development of business and cost models, supply chains implementation, and development, suitability for high volume markets and addressing technology management. Europe already has a vibrant and competitive composites industry that is supported by several research centers, but for its positioning in a forefront position in this technology, further challenges are still required to be addressed.
A Numerical Assessment of Artificial Reef Pass Wave-Induced Currents as a Renewable Energy Source
Authors: Sous Damien
Published: August 2019

The present study aims to estimate the potential of artificial reef pass as a renewable source of energy. The overall idea is to mimic the functioning of natural reef–lagoon systems in which the cross-reef pressure gradient induced by wave breaking is able to drive an outward flow through the pass. The objective is to estimate the feasibility of a positive energy breakwater, combining the usual wave-sheltering function of immersed breakwater together with the production of renewable energy by turbines. A series of numerical simulations is performed using a depth-averaged model to understand the effects of each geometrical reef parameter on the reef–lagoon hydrodynamics. A synthetic wave and tide climate is then imposed to estimate the potential power production. An annual production between 50 and 70 MWh is estimated.
Origin of the Genetic Code Is Found at the Transition between a Thioester World of Peptides and the Phosphoester World of Polynucleotides
Authors: Hartman Hyman , Smith Temple F.
Published: August 2019

The early metabolism arising in a Thioester world gave rise to amino acids and their simple peptides. The catalytic activity of these early simple peptides became instrumental in the transition from Thioester World to a Phosphate World. This transition involved the appearances of sugar phosphates, nucleotides, and polynucleotides. The coupling of the amino acids and peptides to nucleotides and polynucleotides is the origin for the genetic code. Many of the key steps in this transition are seen in the catalytic cores of the nucleotidyltransferases, the class II tRNA synthetases (aaRSs) and the CCA adding enzyme. These catalytic cores are dominated by simple beta hairpin structures formed in the Thioester World. The code evolved from a proto-tRNA, a tetramer XCCA interacting with a proto-aminoacyl-tRNA synthetase (aaRS) activating Glycine and Proline. The initial expanded code is found in the acceptor arm of the tRNA, the operational code. It is the coevolution of the tRNA with the aaRSs that is at the heart of the origin and evolution of the genetic code. There is also a close relationship between the accretion models of the evolving tRNA and that of the ribosome.
First-Principle Calculation of High Absorption-TlGaTe2 for Photovoltaic Application
Authors: Rasukkannu Murugesan , Velauthapillai Dhayalan , Vajeeston Ponniah
Published: August 2019

We use first-principle calculations based on hybrid functional and the Bethe-Salpeter equation method to investigate the electronic and optical properties of dichalcogenide TlGaTe2. Based on theoretical studies, TlGaTe2 has until recently been considered as an indirect band gap material, however; by employing more accurate hybrid functional model, we showed that although TlGaTe2 has an indirect band gap of 1.109 eV, it also exhibits a fundamental direct band gap of 1.129 eV. Our finding was further confirmed by the optical studies on TlGaTe2, which show that the absorption peak is registered at a photon energy of 1.129 eV. It was also shown that TlGaTe2 has high optical absorption peaks in the visible region. Based on phonon and elastic constant calculations, it was shown that TlGaTe2 is dynamically and mechanically stable. Our findings show that TlGaTe2 is a potential candidate for photovoltaic application.
Investigating the Effect of Aggregate Characteristics on the Macroscopic and Microscopic Fracture Mechanisms of Asphalt Concrete at Low-Temperature
Authors: Xu Yinshan , Jiang Yingjun , Xue Jinshun , Ren Jiaolong
Published: August 2019

The low-temperature crack of asphalt concrete is considered to be one of the main deteriorations in asphalt pavements. However, there have been few studies on the composite effects of the aggregate characteristics and fracturing modes on the low-temperature cracking of asphalt concrete. Hence, the edge cracked semi-circular bend tests and the discrete element modeling approaches are combined to investigate the effect of the aggregate contents, aggregate morphological features and aggregate distributions on the fracture behavior of asphalt concrete in different fracturing modes at different temperatures. The results show that the fracture toughness and the crack extended time reduce with the increasing aggregate orientation and flatness and the decreased aggregate content. The effect of aggregate flatness is nonlinear, and its reduction trend grows gradually with the increasing flatness. The total number of failed contacts is reduced with the increasing aggregate orientation and flatness, particularly at 10 °C. The number of failed contacts that occurred in the aggregate–mastic interface in Quasi-Mode II fracturing is slightly higher than that in other fracturing modes. The aggregate distribution in the crack initiation zone greatly influenced the crack resistance, particularly at 10 °C. The research is beneficial to better understand the fracture mechanisms of asphalt concrete at low-temperature.
How to Choose the Superconducting Material Law for the Modelling of 2G-HTS Coils
Authors: Robert Bright Chimezie , Fareed Muhammad Umar , Ruiz Harold Steven
Published: August 2019

In an attempt to unveil the impact of the material law selection on the numerical modelling and analysis of the electromagnetic properties of superconducting coils, in this paper we compare the four most common approaches to the E-J power laws that serve as a modelling tool for the conductivity properties of the second generation of high-temperature superconducting (2G-HTS) tapes. The material laws considered are: (i) the celebrated E-J critical-state like-model, with constant critical current density and no dependence with the magnetic field; (ii) the classical Kim’s model which introduces an isotropic dependence with the environment magnetic field; (iii) a semi-empirical Kim-like model with an orthonormal field dependence, J c ( B ) , widely used for the modelling of HTS thin films; and (iv) the experimentally measured E–J material law for SuperPower Inc. 2G-HTS tapes, which account for the magneto-angular anisotropy of the in-field critical current density J c ( B ; θ ) , with a derived function similar to Kim’s model but taking into account some microstructural parameters, such as the electron mass anisotropy ratio ( γ ) of the superconducting layer. Particular attention has been given to those physical quantities which within a macroscopic approach can be measured by well-established experimental setups, such as the measurement of the critical current density for each of the turns of the superconducting coil, the resulting distribution of magnetic field, and the curve of hysteretic losses for different amplitudes of an applied alternating transport current at self-field conditions. We demonstrate that although all these superconducting material laws are equally valid from a purely qualitative perspective, the critical state-like model is incapable of predicting the local variation of the critical current density across each of the turns of the superconducting coil, or its non-homogeneous distribution along the width of the superconducting tape. However, depending on the physical quantity of interest and the error tolerance allowed between the numerical predictions and the experimental measurements, in this paper decision criteria are established for different regimes of the applied current, where the suitability of one or another model could be ensured, regardless of whether the actual magneto angular anisotropy properties of the superconducting tape are known.
Surface Modification of Pure Magnesium Mesh for Guided Bone Regeneration: In Vivo Evaluation of Rat Calvarial Defect
Authors: Wu Shuang , Jang Yong-Seok , Kim Yu-Kyoung , Kim Seo-Young , Ko Seung-O , Lee Min-Ho
Published: August 2019

Guided bone regeneration is a therapeutic method that uses a barrier membrane to provide space available for new bone formation at sites with insufficient bone volume. Magnesium with excellent biocompatibility and mechanical properties has been considered as a promising biodegradable material for guided bone regeneration; however, the rapid degradation rate in the physiological environment is a problem to be solved. In this study, surface modification of pure magnesium mesh was conducted by plasma electrolytic oxidation and hydrothermal treatment to form a densely protective layer on the Mg substrate. The protective layer mainly consisted of Mg(OH)2 with the amorphous calcium phosphate. Then, weight loss measurement and Micro-CT imaging were performed after an immersion test in a simulated body fluid. The effect of surface modification of the magnesium mesh on the guided bone regeneration was evaluated through an in vivo test using the rat calvarial defect model. The biodegradation of the magnesium mesh was identified to be significantly retarded. Additionally, the surface modification of Mg also can improve the bone volume and bone density of calvarial defect in comparison with that of the pristine Mg mesh.
Strength without Size Effect and Formula of Strength for Concrete and Natural Marble
Authors: Zhou Guangchun , Shi Jun , Yu Maohong , Zhang Yu , Li Xiaochun , Zhao Yan
Published: August 2019

Throughout the several-hundred-year-long history of the concept of strength, inaccurate material strength as a result of the size effect and the inconsistency of strength theories have been two continuous and challenging issues, and have even been taken to be inherent attributes of material strength. Applying the structural stressing state theory and method, this study experimentally investigates the uniaxial load-bearing process of concrete specimens and reveals their stressing state mutation features at specific load levels. Exploration of this general feature resulted in the discovery of essential strength, which is basically without size effect. Then, biaxial and triaxial experiments with concrete specimens were conducted in order to obtain the results for various combinations of principal stresses on essential strength. Consequently, according to Yu’s unified strength theory, the formula for strength of concrete was determined by fitting the relation between the combined principal stresses and the essential strength, which was verified by experiments carried out using natural marble specimens. Essential strength could promote the accuracy of strength indices, and the formula for strength might replace the existing strength theories for brittle materials. The initial solution of these two classic issues could make a new contribution to Yu’s unified strength theory and its final goal, promoting related research on material strength and leading to a more rational use of material strength in practical engineering.
Celebrations for the 50-Year Anniversary of IFToMM
Authors: Ceccarelli Marco , Huang Tian , Zielinska Teresa , Carretero Juan Antonio , Uhl Tadeusz
Published: August 2019

In this paper celebrations of the fifty-years anniversary of IFToMM, the International Federation for the Promotion of Mechanism and Machine Science, is reported with main aspects of the event that has been organized on 30 June 2019 during a special session at the IFToMM World Congress in Krakow, Poland. Memories of the celebration are illustrated with photos and historical data.
n0-Order Weighted Pseudo Δ-Almost Automorphic Functions and Abstract Dynamic Equations
Authors: Wang Chao , Agarwal Ravi P. , O’Regan Donal , N’Guérékata Gaston M.
Published: August 2019

In this paper, we introduce the concept of a n 0 -order weighted pseudo Δ n 0 δ -almost automorphic function under the matched space for time scales and we present some properties. The results are valid for q-difference dynamic equations among others. Moreover, we obtain some sufficient conditions for the existence of weighted pseudo Δ n 0 δ -almost automorphic mild solutions to a class of semilinear dynamic equations under the matched space. Finally, we end the paper with a further discussion and some open problems of this topic.
α-Conotoxin TxIB: A Uniquely Selective Ligand for α6/α3β2β3 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Conditioned Place Preference in Mice
Authors: You Shen , Li Xiaodan , Xiong Jian , Zhu Xiaoyu , Zhangsun Dongting , Zhu Xiaopeng , Luo Sulan
Published: August 2019

α-Conotoxin TxIB is a specific antagonist of α6/α3β2β3(α6β2*) nicotinic acetylcholine receptor (nAChR) with an IC50 of 28 nM. Previous studies have shown that α6β2* nAChRs are abundantly expressed in midbrain dopaminergic neurons and play an important role in mediating the mechanism of nicotine and other drugs reward effect. It provided important targets for the development of anti-addiction drugs. The present study evaluated the pharmacological activity of TxIB in vivo with conditioned place preference (CPP) model, which were induced by subcutaneous injection (s.c.) of nicotine (NIC, 0.5 mg/kg). α-Conotoxin TxIB inhibited the expression and reinstatement of CPP in mice dose-dependently, but had no significant effect on locomotor activity. The concentrations of dopamine (DA), γ-aminobutyric acid (GABA) and noradrenaline (NE) in different brain regions were measured by enzyme-linked immunosorbent assay (ELISA). We found that TxIB could inhibit the concentrations of DA, GABA and NE in different brain regions (such as nucleus accumbens (NAc), hippocampus (HIP) and prefrontal cortex (PFC)) in NIC-induced mice. The concentrations of DA and NE were decreased in ventral tegmental area (VTA), while GABA had little change. The current work described the inhibition activity of TxIB in NIC-induced CPP, suggesting that α6β2* nAChR-targeted compound may be a promising drug for nicotine addiction treatment.
Physiological Gait versus Gait in VR on Multidirectional Treadmill—Comparative Analysis
Authors: Jochymczyk-Woźniak Katarzyna , Nowakowska Katarzyna , Polechoński Jacek , Sładczyk Sandra , Michnik Robert
Published: August 2019

Background and objectives: Virtual reality (VR) is increasingly often finding applications in physiotherapy and health promotion. Recent years have seen the use of advanced technologies in the promotion of physical activity (PA) in society. New simulators, e.g., treadmills, enable the performance of PA (e.g., locomotive movements) in VR (artificially created virtual world). The question of how such movements are similar to natural forms of human locomotion (march, run) inspired the comparative analysis of physiological gait and gait in VR on a multidirectional Omni treadmill. Materials and Methods: The tests involved the use of the BTS Smart system for the triplanar analysis of motion. The test involved 10 healthy females aged 20–24 (weight: 52 ± 3.1 kg, height 162 ± 5.4 cm). Measurements were performed at two stages. The first stage involved the standard assessment of physiological gait, whereas the second was focused on gait forced by the Omni treadmill. The following gait parameters were analyzed: Flexion-extension in the ankle, knee joint and hip joint, rotation in the hip joint and knee joint, foot progression, adduction-abduction in the knee joint and hip joint, pelvic obliquity, pelvic tilt, pelvic rotation as well as energy expenditure and the movement of the body center of mass. Results: The analysis of the test results revealed the existence of differences in the kinematics of physical gait and gait on the treadmill. The greatest differences were recorded in relation to the dorsal-plantar flexion in the ankle, the foot progression, the rotation of the knee joint, pelvic tilt and rotation. In addition, the gait on the treadmill is characterized by the longer duration of the stance phase and reduced ranges of the following movements: Flexion-extension in the ankle, knee joint and hip joint, adduction-abduction in the hip joint as well as rotation in the ankle and hip joint. The values of potential, kinetic and total energy recorded in relation to forced gait are significantly lower than those of physiological gait. Conclusions: Because of the fact that the parameters of gait on the Omni platform vary significantly from the parameters of physical gait, the application of the Omni treadmill in the re-education of gait during rehabilitation should be treated with considerable care. Nonetheless, the treadmill has adequate potential to become a safe simulator enabling active motion in VR using locomotive movements.
Antiviral Activity of Exopolysaccharides Produced by Lactic Acid Bacteria of the Genera Pediococcus, Leuconostoc and Lactobacillus against Human Adenovirus Type 5
Authors: Biliavska Liubov , Pankivska Yulia , Povnitsa Olga , Zagorodnya Svitlana
Published: August 2019

Background and objectives: The use of antagonistic probiotic microorganisms and their byproducts represents a promising approach for the treatment of viral diseases. In the current work, the effect of exopolysaccharides (EPSs) produced by lactic acid bacteria from different genera on the structural and functional characteristics of cells and the development of adenoviral infection in vitro was studied. Materials and Methods: Cytotoxicity of six EPSs of lactic acid bacteria of the genera Lactobacillus, Leuconostoc and Pediococcus was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The influence of the EPSs on the infectivity of human adenovirus type 5 (HAdV-5) and on the cell cycle under a condition of adenovirus infection was studied using plaque reduction assay and flow cytometric analysis, respectively. Results: It was shown that exopolysaccharides were non-toxic to Madin-Darby bovine kidney cells (MDBK) as they reduced their viability by 3–17%. A change in the distribution of the cell cycle phases in the non-infected cell population treated with EPSs was observed. The analysis demonstrated an increase in the number of cells in the S phase by 47% when using EPSs 15a and a decrease in the number of cells in the G1 phase by 20–27% when treated with the EPSs 15a, 33a, and 19s. The use of EPSs did not led to the normalization of the life cycle of HAdV-5 infected cells to the level of non-infected cells. The EPSs showed low virucidal activity and reduced the HAdV-5 infectivity to 85%. Among the studied exopolysaccharides, anti-adenovirus activity was found for EPS 26a that is produced by Lactobacillus spp. strain. The treatment of cells with the EPS following virus adsorption completely (100%) suppressed the formation and release of HAdV-5 infectious. Conclusions: EPS 26a possessed distinct anti-HAdV-5 activity and the obtained data demonstrate the potential of using exopolysaccharides as anti-adenoviral agents.
Validation of Laser Capture Microdissection Protocol in Endometriosis Studies
Authors: da Costa Katiane de Almeida , Malvezzi Helena , Viana Bruno Gallani , Filippi Renée Zon , Neme Rosa Maria , Aloia Thiago Pinheiro Arrais , Podgaec Sérgio , Piccinato Carla de Azevedo
Published: August 2019

Background and Objectives: The presence of endometrial-like tissue outside the uterine cavity is a key feature of endometriosis. Although endometriotic lesions appear to be histologically quite similar to the eutopic endometrium, detailed studies comparing both tissues are required because their inner and surrounding cellular arrangement is distinct. Thus, comparison between tissues might require methods, such as laser capture microdissection (LCM), that allow for precise selection of an area and its specific cell populations. However, it is known that the efficient use of LCM depends on the type of studied tissue and on the choice of an adequate protocol. Recent studies have reported the use of LCM in endometriosis studies. The main objective of the present study is to establish a standardized protocol to obtain good-quality microdissected material from eutopic or ectopic endometrium. Materials and Methods: The main methodological steps involved in the processing of the lesion samples for LCM were standardized to yield material of good quality to be further used in molecular techniques. Results: We obtained satisfactory results regarding the yields and integrity of RNA and protein obtained from LCM-processed endometriosis tissues. Conclusion: LCM can provide more precise analysis of endometriosis biopsies, provided that key steps of the methodology are followed.
Novel Characteristics of Race-Specific Genetic Functions in Korean CADASIL
Authors: Kim Yerim , Lee Seung-Hoon
Published: August 2019

Background and Objectives: Previous studies found differences in the characteristics of NOTCH3 mutations in Caucasians and Asians with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Therefore, we sought to investigate the correlations between genetic and clinical/radiological findings in Korean CADASIL patients including some variants of unknown significance (VUS). Materials and Methods: We screened 198 patients with a suspected diagnosis of CADASIL between 2005 and 2015 via Sanger sequencing. Results: A total of 34 subjects (52.5 ± 9.5 years) were included. The majority of the mutations were in exon 3 and exon 11. R75P mutations (n = 5), followed by Y465C and R544C mutations (n = 4) were the most prevalent. Patients with those mutations exhibited less frequent anterior temporal (AT) or external capsular (EC) hyperintensities compared to patients with other locus mutations. Hemorrhagic stroke (HS) was found to be associated with mutations in exon 3 (R75P), exon 9 (Y465C), exon 11 (R587C), and exon 22 (R1175W variants), which were common locations in our study. Although it is unclear that genetic differences might affect the phenotypes in ethnicities, Asian population shows less migraine or seizure, but more intracerebral hemorrhage. Unlike in westernized countries, typical AT or EC hyperintensities may not be significant MRI markers, at least in Korean CADASIL patients. Furthermore, similar to R75P phenotypes, it is a novel finding that patients with Y465C and R1175W VUS have less frequent AT involvement than Caucasians. Conclusion: The associations between HS and common genetic locations account for the increased development of intracerebral hemorrhage in Koreans rather than Caucasians. We suggest that some CADASIL mutations appear to impart novel region-specific characteristics.
Plant Tissue Localization and Morphological Conversion of Azospirillum brasilense upon Initial Interaction with Allium cepa L.
Authors: Hong Leidong , Orikasa Yoshitake , Sakamoto Hisayo , Ohwada Takuji
Published: August 2019

The genus Azospirillum is recognized as plant growth-promoting bacteria that exert beneficial effects on the host plant and is morphologically converted into cyst-like cells (i.e., c-form) in association with poly-β-hydroxybutyrate (PHB) accumulation in the cells under stress conditions. We constructed Azospirillum brasilense, labeled with reporter genes (gus/gfp, mCherry) and examined the plant tissue localization along with a morphological conversion into the c-form upon its initial interaction with onion seedlings (Allium cepa L.). The PHB granules in the A. brasilense cells were easily detected under fluorescence as “black holes”, rendering it possible to monitor the morphological conversion from vegetative to the c-form cells. The results showed that the A. brasilense cells on the surface of the roots and bulbs (underground stem) began converting at three days following inoculation and that the cell conversion was significantly advanced with time along with the cell population increase. The endophytic infection of A. brasilense into the bulb tissues was also confirmed, although these likely constituted vegetative cells. Moreover, the morphological conversion into the c-form was induced under nitrogen-restricted conditions. Analysis of the biochemical properties of the A. brasilense cells during cell conversion revealed that the acetylene reduction activity correlated positively with the PHB accumulation in the cells converting into the c-form under nitrogen-restricted conditions.
Disinfectant Susceptibility of Biofilm Formed by Listeria monocytogenes under Selected Environmental Conditions
Authors: Skowron Krzysztof , Wałecka-Zacharska Ewa , Grudlewska Katarzyna , Gajewski Piotr , Wiktorczyk Natalia , Wietlicka-Piszcz Magdalena , Dudek Andżelika , Skowron Karolina Jadwiga , Gospodarek-Komkowska Eugenia
Published: August 2019

Listeria monocytogenes is a one of the most important food-borne pathogens. Its ability to form biofilm contributes to increased resistance to disinfectants and inefficient disinfection, posing a serious threat for the food industry, and in the end the consumer. The aim of this study was the comparison of the biofilm formation ability of L. monocytogenes strains on stainless steel, under different environmental conditions (temperature, pH, NaCl concentration, nutrients availability), and the assessment of biofilm susceptibility to disinfectants. The bactericidal activity of four disinfectants in two concentrations (100% and 50% of working solution) against biofilm was conducted on four clinical strains, four strains isolated from food and one reference strain ATCC 19111. It was found that biofilm susceptibility to disinfectants was influenced by environmental conditions. Biofilm susceptibility correlated with the decrease of temperature, pH, nutrients availability and salinity of the environment. The least sensitive to disinfectants was biofilm produced at pH = 4 (the bacterial number ranged from 0.25 log CFU × cm−2 to 1.72 log CFU × cm−2) whereas the most sensitive was biofilm produced at pH = 9 (5.16 log CFU × cm−2 to 7.84 log CFU × cm−2). Quatosept was the most effective disinfectant, regardless of the conditions. In conclusion, biofilm susceptibility to disinfectants is strain-dependent and is affected by environmental conditions.
Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
Authors: Hernández-Arriaga Angélica , Baumann Anja , Witte Otto W. , Frahm Christiane , Bergheim Ina , Camarinha-Silva Amélia
Published: August 2019

The mouth is an important niche for bacterial colonization. Previous research used mouth microbiota to predict diseases like colon cancer and inflammatory bowel disease (IBD). It is still unclear how the sampling methodology influences microbial characterization. Our aim was to determine if the sampling methods, e.g., cotton swab or tissue biopsy, and the age influence the oral microbial composition of mice. Microbial DNA was extracted using a commercial kit and characterized targeting the 16s rRNA gene from mouth swabs and tissue biopsies from 2 and 15 months old C57BL/6 male mice kept in the same SPF facility. Our results show statistical different microbial community of the different ages, type of sampling, and the two fixed factors age x type of sample (p-value < 0.05). At the genus level, we identified that the genera Actinobacillus, Neisseria, Staphylococcus, and Streptococcus either increase or decrease in abundance depending on sampling and age. Additionally, the abundance of Streptococcus danieliae, Moraxella osloensis, and some unclassified Streptococcus was affected by the sampling method. While swab and tissue biopsies both identified the common colonizers of oral microbiota, cotton swabbing is a low-cost and practical method, validating the use of the swab as the preferred oral sampling approach.
Rare Earth Elements (REE) in Al- and Fe-(Oxy)-Hydroxides in Bauxites of Provence and Languedoc (Southern France): Implications for the Potential Recovery of REEs as By-Products of Bauxite Mining
Authors: Mondillo Nicola , Balassone Giuseppina , Boni Maria , Chelle-Michou Cyril , Cretella Salvatore , Mormone Angela , Putzolu Francesco , Santoro Licia , Scognamiglio Gennaro , Tarallo Marcella
Published: August 2019

Bauxites in southern France (Provence and Languedoc) have been exploited since the beginning of the last century. Though most of the deposits are now subeconomic or mined-out, these bauxites represent model analogs for other economic bauxites of the world. These Cretaceous karst-type deposits lie directly on Jurassic carbonates, and have been formed through a combination of different processes: in-situ alteration of siliciclastic sediments deposited on carbonate platforms, and reworking of early bauxites in the karst network. In this study, we present preliminary bulk rock geochemical and in-situ laser ablation (LA) -ICP-MS analyses on Al- and Fe-oxy-hydroxides of Provence (Les Baux-de-Provence) and Languedoc (Villeveyrac, Loupian) bauxites, with the aim of evaluating the concentrations of rare earth elements (REEs) and their deportment in these minerals. REEs have total average concentrations of 700 mg/kg in the analyzed samples, which are mostly composed of boehmite, γ-AlO(OH), and Fe-oxy-hydroxides (hematite and goethite). Maximum REEs concentrations are commonly associated with positive Ce anomalies in chondrite-normalized patterns. In contrast with other examples from the literature, it has been observed that high REE concentrations also occur in samples apparently devoid or poor of REE-minerals. In these samples, the total amount of REEs is positively correlated with that of Ga (commonly contained in boehmite). LA-ICP-MS trace element analyses on boehmite and Fe-oxy-hydroxides have shown that while the Al-hydroxide contains the suite of REEs, goethite and hematite are preferentially enriched only in Ce. Considering that Al-hydroxides are digested during the Bayer process, an interesting issue to develop in the future is whether (and how) REEs released during Al-hydroxide digestion could be recovered together with Al from the pregnant leach liquor, as routinely done for Ga.
The Importance of Reaction Conditions on the Chemical Structure of N,O-Acylated Chitosan Derivatives
Authors: Piegat Agnieszka , Goszczyńska Agata , Idzik Tomasz , Niemczyk Agata
Published: August 2019

The structure of acylated chitosan derivatives strongly determines the properties of obtained products, influencing their hydrodynamic properties and thereby their solubility or self-assembly susceptibility. In the present work, the significance of slight changes in acylation conditions on the structure and properties of the products is discussed. A series of chitosan-acylated derivatives was synthesized by varying reaction conditions in a two-step process. As reaction media, two diluted acid solutions—i.e., acetic acid and hydrochloric acid)—and two coupling systems—i.e., 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride (EDC) and N–hydroxysulfosuccinimide (EDC/NHS)—were used. The chemical structure of the derivatives was studied in detail by means of two spectroscopic methods, namely infrared and nuclear magnetic resonance spectroscopy, in order to analyze the preference of the systems towards N- or O-acylation reactions, depending on the synthesis conditions used. The results obtained from advanced 1H-13C HMQC spectra emphasized the challenge of achieving a selective acylation reaction path. Additionally, the study of the molecular weight and solution behavior of the derivatives revealed that even slight changes in their chemical structure have an important influence on their final properties. Therefore, an exact knowledge of the obtained structure of derivatives is essential to achieve reaction reproducibility and to target the application.
Protective Responses Induced by Chiral 3-Dichloroacetyl Oxazolidine Safeners in Maize (Zea mays L.) and the Detoxification Mechanism
Authors: Gao Shuang , Liu Yan-Yan , Jiang Jing-Yu , Fu Ying , Zhao Li-Xia , Li Chun-Yan , Ye Fei
Published: August 2019

Herbicide safeners selectively protect crops from herbicide injury while maintaining the herbicidal effect on the target weed. To some extent, the detoxification of herbicides is related to the effect of herbicide safeners on the level and activity of herbicide target enzymes. In this work, the expression of the detoxifying enzyme glutathione S-transferase (GST) and antioxidant enzyme activities in maize seedlings were studied in the presence of three potential herbicide safeners: 3-dichloroacetyl oxazolidine and its two optical isomers. Further, the protective effect of chiral herbicide safeners on detoxifying chlorsulfuron in maize was evaluated. All safeners increased the expression levels of herbicide detoxifying enzymes, including GST, catalase (CAT), and peroxidase (POD) to reduce sulfonylurea herbicide phytotoxicity in maize seedlings. Our results indicate that the R-isomer of 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine can induce glutathione (GSH) production, GST activity, and the ability of GST to react with the substrate 1-chloro-2,4-dinitrobenzene (CDNB) in maize, meaning that the R-isomer can protect maize from damage by chlorsulfuron. Information about antioxidative enzyme activity was obtained to determine the role of chiral safeners in overcoming the oxidative stress in maize attributed to herbicides. The interaction of safeners and active target sites of acetolactate synthase (ALS) was demonstrated by molecular docking modeling, which indicated that both isomers could form a good interaction with ALS. Our findings suggest that the detoxification mechanism of chiral safeners might involve the induction of the activity of herbicide detoxifying enzymes as well as the completion of the target active site between the safener and chlorsulfuron.
Computational Approaches for Drug Discovery
Authors: Brogi Simone
Published: August 2019

Computational approaches represent valuable and essential tools in each step of the drug discovery and development trajectory [...]
The Effects of Chloride Flux on Drosophila Heart Rate
Authors: Stanley Catherine E. , Mauss Alex S. , Borst Alexander , Cooper Robin L.
Published: August 2019

Approaches are sought after to regulate ionotropic and chronotropic properties of the mammalian heart. Electrodes are commonly used for rapidly exciting cardiac tissue and resetting abnormal pacing. With the advent of optogenetics and the use of tissue-specific expression of light-activated channels, cardiac cells cannot only be excited but also inhibited with ion-selective conductance. As a proof of concept for the ability to slow down cardiac pacing, anion-conducting channelrhodopsins (GtACR1/2) and the anion pump halorhodopsin (eNpHR) were expressed in hearts of larval Drosophila and activated by light. Unlike body wall muscles in most animals, the equilibrium potential for Cl− is more positive as compared to the resting membrane potential in larval Drosophila. As a consequence, upon activating the two forms of GtACR1 and 2 with low light intensity the heart rate increased, likely due to depolarization and opening of voltage-gated Ca2+ channels. However, with very intense light activation the heart rate ceases, which may be due to Cl– shunting to the reversal potential for chloride. Activating eNpHR hyperpolarizes body wall and cardiac muscle in larval Drosophila and rapidly decreases heart rate. The decrease in heart rate is related to light intensity. Intense light activation of eNpHR stops the heart from beating, whereas lower intensities slowed the rate. Even with upregulation of the heart rate with serotonin, the pacing of the heart was slowed with light. Thus, regulation of the heart rate in Drosophila can be accomplished by activating anion-conducting channelrhodopsins using light. These approaches are demonstrated in a genetically amenable insect model.