bluuebunny/crossref_metadata_2025 huggingface dataset:
Information Bottleneck Classification in Extremely Distributed Systems
Authors: Ullmann Denis , Rezaeifar Shideh , Taran Olga , Holotyak Taras , Panos Brandon , Voloshynovskiy Slava
Published: October 2020

We present a new decentralized classification system based on a distributed architecture. This system consists of distributed nodes, each possessing their own datasets and computing modules, along with a centralized server, which provides probes to classification and aggregates the responses of nodes for a final decision. Each node, with access to its own training dataset of a given class, is trained based on an auto-encoder system consisting of a fixed data-independent encoder, a pre-trained quantizer and a class-dependent decoder. Hence, these auto-encoders are highly dependent on the class probability distribution for which the reconstruction distortion is minimized. Alternatively, when an encoding–quantizing–decoding node observes data from different distributions, unseen at training, there is a mismatch, and such a decoding is not optimal, leading to a significant increase of the reconstruction distortion. The final classification is performed at the centralized classifier that votes for the class with the minimum reconstruction distortion. In addition to the system applicability for applications facing big-data communication problems and or requiring private classification, the above distributed scheme creates a theoretical bridge to the information bottleneck principle. The proposed system demonstrates a very promising performance on basic datasets such as MNIST and FasionMNIST.
Effects of Building Energy Efficiency Measures on Air Quality at the Neighborhood Level in Athens, Greece
Authors: Frilingou Natasha , Bouris Demetri
Published: October 2020

The high concentration of pollutant sources, complex topography, and regional meteorology are all factors that may contribute to air episodes in dense urban areas. Energy use in buildings is a significant source of pollution in the Greater Athens Area (GAA), Greece, where over 90% of the existing building stock has been classified below energy class B. The present study focuses on the potential effects that a realistic level of building energy efficiency upgrades will have on the air quality over the GAA. Results are expected to be relevant to similar urban areas. Furthermore, the study of primary pollutants’ dispersion is applied at a 1.2 × 1.2 km spatial resolution, providing significant local (neighborhood) level information. Numerical simulations were performed using EPA’s CALPUFF modeling system with wind field input from an independent numerical weather prediction using NCAR’s Weather Research and Forecasting (WRF) model. In order to calculate emission rates from major roads, highways, shipping ports, residential heating installations, and major industrial facilities, data were taken from National and European statistics, demographics, and local topography. After validation, the modeling system was used to examine three building energy efficiency upgrade scenarios, implemented on 20% of the buildings. Ground level concentrations of SO2, NOx, CO, and PM10 were calculated and reductions of up to 9% were found for GAA maximum values but up to 18% for local values that were also close to or above the European safety thresholds.
Dispersion Effects of Particulate Lead (Pb) from the Stack of a Lead Battery Recycling Plant
Authors: Karali Dimitra , Stavridis Alexandros , Loupa Glykeria , Rapsomanikis Spyridon
Published: October 2020

The contribution of emissions from the stack of a lead battery recycling plant to atmospheric lead concentrations and, eventually, to the topsoil of the surrounding area, were studied. A Gaussian dispersion model, of the American Meteorological Society/United States Environmental Protection Agency Regulatory Model, (AERMOD) was used to determine atmospheric total suspended particulate lead dispersion, which originated from stack emissions, over the wider study area. Stack emission parameters were obtained from online measurements of the industry control sensors. AERMOD simulated two scenarios for four calendar years, 2015 to 2018, one for the typical stack measured operating conditions and one for the legal limit operating conditions (emissions from the stack set by legislation to 0.5 mg m−3). Deposition fluxes modeled the input of atmospheric total suspended particulate Pb to the topsoil of the area. X-ray fluorescence (XRF) analyses were used to determine lead concentrations in the topsoil. The modeling results were compared with topsoil of six inhabited locations downwind from the stack in the direction of the prevailing winds to estimate the influence of lead deposition on topsoil near the industrial area.
CO2 Capture, Use, and Storage in the Cement Industry: State of the Art and Expectations
Authors: Plaza Marta G. , Martínez Sergio , Rubiera Fernando
Published: October 2020

The implementation of carbon capture, use, and storage in the cement industry is a necessity, not an option, if the climate targets are to be met. Although no capture technology has reached commercial scale demonstration in the cement sector yet, much progress has been made in the last decade. This work intends to provide a general overview of the CO2 capture technologies that have been evaluated so far in the cement industry at the pilot scale, and also about the current plans for future commercial demonstration.
CFD Modeling of the Catalyst Oil Slurry Hydrodynamics in a High Pressure and Temperature as Potential for Biomass Liquefaction
Authors: Wodołażski Artur , Skiba Jacek , Zarębska Katarzyna , Polański Jarosław , Smolinski Adam
Published: October 2020

The paper presents the simulation of a catalyst-paraffin oil slurry hydrodynamics under high pressure and temperature in a convex bottom reactor with a Rushton turbine which was conducted with an application of computational fluid dynamics (CFD) modeling. An analysis to obtain a uniform distribution of solid catalyst particles suspended in paraffin oil was carried out as a potential for biomass liquefaction. The effects of the particle diameter, bed density, liquid viscosity, and the initial solid loading on slurry hydrodynamics in high pressure and temperature behavior were investigated using the Eulerian–Eulerian two-fluid model and the standard k-ε turbulence model. The main objective was to assess the performance in agitating highly concentrated slurries to obtain slurry velocity, concentration, the degree of homogeneity, and to examine their effect on the mixing quality. The results of the analysis are applied to predicting the impact of the most efficient conditions on slurry suspension qualities as potential for biomass liquefaction.
Design of an Optimized Thermal Management System for Li-Ion Batteries under Different Discharging Conditions
Authors: Bhattacharjee Ankur , Mohanty Rakesh K. , Ghosh Aritra
Published: October 2020

The design of an optimized thermal management system for Li-ion batteries has challenges because of their stringent operating temperature limit and thermal runaway, which may lead to an explosion. In this paper, an optimized cooling system is proposed for kW scale Li-ion battery stack. A comparative study of the existing cooling systems; air cooling and liquid cooling respectively, has been carried out on three cell stack 70Ah LiFePO4 battery at a high discharging rate of 2C. It has been found that the liquid cooling is more efficient than air cooling as the peak temperature of the battery stack gets reduced by 30.62% using air cooling whereas using the liquid cooling method it gets reduced by 38.40%. The performance of the liquid cooling system can further be improved if the contact area between the coolant and battery stack is increased. Therefore, in this work, an immersion-based liquid cooling system has been designed to ensure the maximum heat dissipation. The battery stack having a peak temperature of 49.76 °C at 2C discharging rate is reduced by 44.87% to 27.43 °C after using the immersion-based cooling technique. The proposed thermal management scheme is generalized and thus can be very useful for scalable Li-ion battery storage applications also.
MELCOR Analysis of a SPARC Experiment for Spray-PAR Interaction during a Hydrogen Release
Authors: Kim Hyoung Tae , Kim Jongtae
Published: October 2020

A series of experiments were performed in the SPARC (spray-aerosol-recombiner-combustion) test facility to simulate a hydrogen mitigation system with the actuation of a PAR (passive auto-catalytic re-combiner) and spray system. In this study, the SPARC-SPRAY-PAR (SSP1) experiment is chosen to benchmark the MELCOR (a lumped-parameter code for severe accident analysis) predictions against test data. For this purpose, firstly we prepared the base input model of the SPARC test vessel, and tested it by a simple verification problem with well-defined boundary conditions. The implementation of a currently used PAR correlation in MELCOR is shown to be appropriate for the simulation of a PAR actuation experiment. In an SSP1 experiment, the PAR is reacting with hydrogen, and the spray actuation starts as soon as hydrogen injection is complete. The MELCOR simulation well predicts the pressure behavior and the gas flow affected by operating both a PAR and spray system. However, the local hydrogen concentration measurement near the inlet nozzle is much higher than the volume average-value by MELCOR, since high jet flow from the nozzle is dispersed in the corresponding cell volume. The experimental reproduction of the phenomena we expect, or, conversely, the identification of phenomena we do not understand, will continue to support the verification of analytical models using experimental data and to analyze the impact of spray on PAR operations in severe accident conditions.
Operation and Sensitivity Analysis of a Micro-Scale Hybrid Trigeneration System Integrating a Water Steam Cycle and Wind Turbine under Different Reference Scenarios
Authors: Figaj Rafał , Sornek Krzysztof , Podlasek Szymon , Żołądek Maciej
Published: October 2020

Renewable energy sources, such as solar, wind, biomass, and geothermal energy, are being more and more adopted in small and micro-scale distributed generation systems. In this context, different hybrid configurations and layouts that may adopt, lead to different energy and economic performance of energy generation systems. In micro-scale applications, biomass and solar energy sources are more frequently investigated in literature compared to other combinations as biomass and wind energy. The analysis of the performance of a novel small-scale trigeneration system is presented in this paper. The system includes biomass boiler, water steam turbine, absorption chiller, and wind turbine, and it is linked to the electric grid by means of a bidirectional connection, allowing to the store virtually the electrical energy produced in excess, and use when needed. For the proposed system, a zootechnical farm and a residential building are considered as case study, including different scenarios for the reference energy system. The Transient System Simulation (TRNSYS software is used to model, simulate, and investigate the system performance under realistic operation conditions. Energy and economic performance of the system is assessed by means of a daily, weekly, and yearly analysis. The effect of the main design parameters, as steam and wind turbine power on the system performance, is investigated by means of a sensitivity analysis. The investigations show that the Simple Pay Back time of the proposed system is below 6 years, when the biomass is free, capacities of steam and wind turbines lower than 4 kW are selected, and a reference system with a natural gas boiler and electrical grid is considered. The system allows one to achieve satisfactory energy and economic performance under the considered conditions, when a proper design of the system component capacities is adopted.
Power Quality Measurement and Active Harmonic Power in 25 kV 50 Hz AC Railway Systems
Authors: Seferi Yljon , Blair Steven M. , Mester Christian , Stewart Brian G.
Published: October 2020

Railway electrical networks rated at 25 kV 50 Hz are characterised by significant levels of voltage and current harmonics. These frequency components are also time varying in nature due to the movement of trains and changing operational modes. Processing techniques used to evaluate harmonic results, although standardised, are not explicitly designed for railway applications, and the smoothing effect that the standard aggregation algorithms have on the measured results is significant. This paper analyses the application accuracy of standardised power quality (PQ) measurement algorithms, when used to measure and evaluate harmonics in railway electrical networks. A shorter aggregation time interval is proposed for railway power quality measurement instruments, which offers more accurate estimated results and improved tracking of time varying phenomena. Harmonic active power present in railway electrical networks is also evaluated in order to quantify the impact it has on the energy accumulated by electrical energy meters installed on-board trains. Analysis performed on 12 train journeys shows significant levels of non-fundamental active power developed for short periods of time. As an energy meter will inadvertently absorb the financial cost of non-fundamental energy produced by other trains or other external power flows, results are provided to support recommendations for future standards to measure only fundamental frequency energy within train energy measurement meters.
Soil Is a Net Source of Methane in Tropical African Forests
Authors: Castaldi Simona , Bertolini Teresa , Nicolini Giacomo , Valentini Riccardo
Published: October 2020

Research Highlights: Monitoring of soil CH4 fluxes in African tropical forest conducted run for almost two years, contributing to the scant information on greenhouse gas (GHG) fluxes from forests available from this region. Data showed that the forest soil acted as a net yearly source of CH4. Hotspots of CH4 emissions were measured both in upland and lowland areas of the forest, and on an annual basis they overcame the soil CH4 sink during drier periods or in well-drained areas. Background and Objectives: Atmospheric studies indicate that tropics are a strong CH4 source. Regional budgets attribute the majority of this source to wetland ecosystems and flooded lowland forests, whereas un-flooded forests are considered net CH4 sinks, although few studies in tropical forests, in particular in Africa, are available. The present work aims to contribute to this knowledge gap. Materials and Methods: Monitoring campaigns were conducted along the year in the tropical forest of the Ankasa National Park, Ghana, in two contrasting environments, uphill and downhill, using close static chambers coupled with gas chromatography. Results: The uphill area was a net weak CH4 sink with mean daily fluxes ranging from −1.29 to 0.44 mg CH4 m−2 d−1. The downhill area was a significant CH4 source with mean daily fluxes ranging from −0.67 to 188.09 mg CH4 m−2 d−1 and with peaks up to 1312 mg CH4 m−2 d−1 in the wet season. Conclusions: The net annual soil CH4 budget for the Ankasa Park, normalizing the proportion of downhill areas over the whole park surface, was a source of about 3.3 kg CH4 ha−1 yr−1. Overlooking such areas might lead to underestimates of the total CH4 source strength of forested areas.
Predicting the Potential Distribution of Two Varieties of Litsea coreana (Leopard-Skin Camphor) in China under Climate Change
Authors: Pan Jinwen , Fan Xin , Luo Siqiong , Zhang Yaqin , Yao Shan , Guo Qiqiang , Qian Zengqiang
Published: October 2020

Climate change considerably affects vegetation growth and may lead to changes in vegetation distribution. Leopard-skin camphor is an endangered species, and the main raw material for hawk tea, and has various pharmacodynamic functions. Studying the potential distribution of two leopard-skin camphor varieties under climate change should assist in the effective protection of these species. We collected the distribution point data for 130 and 89 Litsea coreana Levl. var. sinensis and L. coreana Levl. var. lanuginosa, respectively, and data for 22 environmental variables. We also predicted the potential distribution of the two varieties in China using the maximum entropy (MaxEnt) model and analyzed the key environmental factors affecting their distribution. Results showed that the two varieties are mainly located in the subtropical area south of the Qinling Mountains–Huai River line in the current and future climate scenarios, and the potentially suitable area for L. coreana Levl. var. lanuginosa is larger than that of L. coreana Levl. var. sinensis. Compared with current climatic conditions, the potentially suitable areas of the two leopard-skin camphor varieties will move to high-latitude and -altitude areas and the total suitable area will increase slightly, while moderately and highly suitable areas will be significantly reduced under future climatic scenarios. For example, under a 2070-RCP8.5 (representative of a high greenhouse gas emission scenario in the 2070s) climatic scenario, the highly suitable areas of L. coreana Levl. var. sinensis and L. coreana Levl. var. lanuginosa are 6900 and 300 km2, and account for only 10.27% and 0.21% of the current area, respectively. Temperature is the key environmental factor affecting the potential distribution of the two varieties, especially the mean daily diurnal range (Bio2) and the min temperature of the coldest month (Bio6). The results can provide a reference for relevant departments in taking protective measures to prevent the decrease or extinction of the species under climate change.
Divergent Mutational Landscapes of Consensus and Minority Genotypes of West Nile Virus Demonstrate Host and Gene-Specific Evolutionary Pressures
Authors: Caldwell Haley S. , Lasek-Nesselquist Erica , Follano Paisley , Kramer Laura D. , Ciota Alexander T.
Published: October 2020

Our current understanding of the natural evolution of RNA viruses comes largely from consensus level genetic analyses which ignore the diverse mutant swarms that comprise within-host viral populations. The breadth and composition of viral mutant swarms impact viral fitness and adaptation, and the capacity for swarm plasticity is likely to be particularly important for arthropod-borne viruses (arboviruses) that cycle between taxonomically divergent hosts. Despite this, characterization of the relationship between the selective pressures and genetic signatures of the mutant swarm and consensus sequences is lacking. To clarify this, we analyzed previously generated whole genome, deep-sequencing data from 548 West Nile virus samples isolated from avian tissues or mosquitoes in New York State from 1999–2018. Both consensus level (interhost) and minority level (intrahost) nucleotide and amino acid sequences were analyzed, and diversity at each position was calculated across the genome in order to assess the relationship between minority and consensus sequences for individual genes and hosts. Our results indicate that consensus sequences are an inept representation of the overall genetic diversity. Unique host and gene-specific signatures and selective pressures were identified. These data demonstrate that an accurate and comprehensive understanding of arbovirus evolution and adaptation within and between hosts requires consideration of minority genotypes.
Banned Books behind Bars: Prototyping a Data Repository to Combat Arbitrary Censorship Practices in U.S. Prisons
Authors: Cauley Kate
Published: October 2020

“Banned Books Behind Bars” is a social justice project that aims to shed light on the complex problem of information access in prison and to explore potential prototypes for possible solutions to some of these obstacles, in particular access to books and printed information. The United States is home to five percent of the world’s population but a staggering twenty-five percent of the world’s total prisoners. For many incarcerated individuals, access to information is a struggle: censorship, book banning, and lack of adequate library facilities or collections are common. Over the course of conducting preliminary research, this project evolved through the research process of ideation. Through the participatory action research method, qualitative interviews with volunteers from banned books organizations helped to identify potential digital tools meant to aid in the fight against the First Amendment violations that incarcerated individuals face daily. Furthermore, the interviews clarified that the first step toward creating an impactful digital project involves converting various forms of unstructured data, including newspaper articles, prison censorship forms, and state published banned book lists, into structured data. Through this discovery, “Banned Books Behind Bars” became an endeavor to standardize practices of data aggregation amongst banned books organizations throughout the country. Gathering concrete data about the practice of banning books within prisons requires an elevated level of transparency. Incarcerated individuals, their families, and prison reform activists need a platform for reporting data on censorship practices, and, ultimately, for bringing awareness to the arbitrary application of censorship guidelines within the complex world of incarceration. The final prototype is a digital repository, created with Airtable software, which offers authoritative dataset consolidation for activists and organizations working to deliver books to prisoners.
Evaluation of Variability in Dietary Quality of School Lunches Meeting National School Lunch Program Guidelines by Socioeconomic Status and Rurality
Authors: Joyce Jillian M. , Rosenkranz Richard R. , Rosenkranz Sara K.
Published: October 2020

Research suggests that the dietary quality (DQ) of school lunches meeting the National School Lunch Program (NSLP) requirements may vary significantly. Possible drivers of variation include factors, such as socioeconomic status (SES) and rurality. The purpose of this cross-sectional study was to determine whether there was variation in nutrient content and DQ by SES and rurality, when analyzing middle school lunch menus meeting NSLP requirements. A random sample of 45 Kansas middle school lunch menus each were obtained from websites of randomly selected districts from low- and high-SES strata. Thirty-day menus were analyzed for nutrient content. Healthy Eating Index (HEI) 2015 scores were calculated for DQ. Rurality was determined for schools by National Center for Education Statistics (NCES) locale. There were significant differences in added sugar (p < 0.001) and calcium (p = 0.001) favoring high-SES menus, and in sodium (p = 0.001) favoring low-SES menus. There were no nutrient differences by rurality. The HEI scores were not different by SES or rurality, with a mean score (SD) 61.9 (2.6) across all schools. Middle school lunch DQ in Kansas does not vary by SES or rurality. Efforts to improve DQ should focus on all foodservice operations, not specifically low-SES or rural schools.
Efficiency of Wastewater Treatment Plants (WWTPs) for Microplastic Removal: A Systematic Review
Authors: Cristaldi Antonio , Fiore Maria , Zuccarello Pietro , Oliveri Conti Gea , Grasso Alfina , Nicolosi Ilenia , Copat Chiara , Ferrante Margherita
Published: October 2020

Plastic is widely used for human activities (food packaging, medical, technological devices, etc.) and there is a growing concern regarding the risks for environmental and human health because they have still not been fully evaluated. Particularly, microplastics (primary and secondary) are present in all environmental compartments and this poses a potential threat because of their entry into the food chain. Furthermore, microplastics can absorb numerous pollutants that can be accumulated in the human body through bioaccumulation and biomagnification processes. We carried out a systematic review using a PRISMA approach to verify the efficiency of wastewater treatment plants (WWTPs) for microplastic removal. The international databases (PubMed, Science Direct, Scopus) were used to find published studies on efficiency of wastewater treatment plants (WWTPs) for microplastic removal. The search period was between January 2010 and June 2020. Over 1000 full research papers were initially selected through the use of keywords. After that, the papers were further selected by English language, title, and abstract, and duplicate papers and non-relevant papers were eliminated according to eligibility criteria. Finally, we included 15 full research papers. In each of the 15 full research papers selected, the microplastics identified were categorized by the authors for shape, size, and type of polymers identified. The characterization of the various types of microplastics was performed by Fourier Transform Infrared Spectroscopy (FTIR) or Raman spectroscopy. We have observed how wastewater treatments plants located in different continents (Europe, Asia, North America) mostly use a primary and secondary type of treatment that allows one to reach a high percentage of microplastics removal from wastewater. Most of the wastewater treatments plants investigated reported a microplastics removal efficiency greater than 90%, but despite this, millions of microplastics continue to be released every day into the aquatic environment. Then, in the near future, efficient and common standardized protocols for monitoring MPs should be drawn up, as well as increasing the knowledge of sources and strategies to further reduce microplastics contamination of treated wastewater.
Effect of per Capita Income on the Relationship between Periodontal Disease during Pregnancy and the Risk of Preterm Birth and Low Birth Weight Newborn. Systematic Review and Meta-Analysis
Authors: Moliner-Sánchez Carmen Alba , Iranzo-Cortés José Enrique , Almerich-Silla José Manuel , Bellot-Arcís Carlos , Ortolá-Siscar José Carmelo , Montiel-Company José María , Almerich-Torres Teresa
Published: October 2020

This work analyzed the available evidence in the scientific literature about the risk of preterm birth and/or giving birth to low birth weight newborns in pregnant women with periodontal disease. A systematic search was carried out in three databases for observational cohort studies that related periodontal disease in pregnant women with the risk of preterm delivery and/or low birth weight, and that gave their results in relative risk (RR) values. Eleven articles were found, meeting the inclusion criteria. Statistically significant values were obtained regarding the risk of preterm birth in pregnant women with periodontitis (RR = 1.67 (1.17–2.38), 95% confidence interval (CI)), and low birth weight (RR = 2.53 (1.61–3.98) 95% CI). When a meta-regression was carried out to relate these results to the income level of each country, statistically significant results were also obtained; on the one hand, for preterm birth, a RR = 1.8 (1.43–2.27) 95% CI was obtained and, on the other hand, for low birth weight, RR = 2.9 (1.98–4.26) 95% CI. A statistically significant association of periodontitis, and the two childbirth complications studied was found, when studying the association between these results and the country’s per capita income level. However, more studies and clinical trials are needed in this regard to confirm the conclusions obtained.
The Impact of Social Factors on Job Crafting: A Meta-Analysis and Review
Authors: Wang Huatian , Li Peikai , Chen Shi
Published: October 2020

Despite the considerable focus on job characteristics and individual differences in job crafting research, the influence of social factors on job crafting has not been well-acknowledged. Based on social interaction and job crafting literature, this meta-analysis estimates the associations between social factors (i.e., organizational insiders and outsiders) and job crafting, and how these social factors contribute to employee outcomes through their job crafting. Based on a sample of 51 empirical studies that included 54 independent samples (N = 17,863), we found that social factors of positive leadership styles (e.g., empowering and transformational) and coworker support were positively related to employee job crafting. Moreover, leadership showed a stronger correlation with employee job crafting than coworker support and Leader-Member-Exchange (LMX). Further, our study showed that employee job crafting positively mediates the relationships between social factors and work outcomes (e.g., job performance and well-being). Our study contributes to job crafting literature by integrating social factors into the job crafting model and demonstrating that the social context of work (in particular organizational insiders) plays a crucial role in shaping employees’ job crafting behavior. We also emphasize the critical role that job crafting plays in transmitting valuable social resources into improved work outcomes. Building on our results, we provide future direction for job crafting research and discuss how our results can imply practice in terms of job crafting training.
What Are the Experiences of Emotional Labor and Workplace Violence that Are More Harmful to Health in Korean Workforce?
Authors: Hwang Won Ju , Yang Hye Kyung , Kim Ji Hye
Published: October 2020

(1) Background: This study aims to investigate the impact of emotional labor and workplace violence on sleep disturbance, depression, and health status in workers. (2) Methods: Data from 34,742 participants of the 2011–2014 Korean Working Conditions Survey were included in this study. We compared the incidence of sleep disturbance, depression, and health status according to emotional labor and workplace violence and used logistic regression to analyze factors that affect health status. (3) Results: Emotional laborers were more likely to experience sleep disturbance, depression and anxiety, and muscle pain. Workers who have experienced workplace violence were more likely to experience depression and anxiety, abdominal pain, and sleep disturbance. (4) Conclusion: Emotional labor and workplace violence have a grave impact on physical and mental health, with particularly greater effects on mental health. In addition, workplace violence has a greater health impact than emotional labor. The findings of this study suggest the need to implement programs that stabilize and heal workers who have experienced emotional labor and to enforce regulations and policies to protect workers from verbal and physical abuse.
Risk Management in First Aid for Acute Drug Intoxication
Authors: Piccioni Andrea , Cicchinelli Sara , Saviano Luisa , Gilardi Emanuele , Zanza Christian , Brigida Mattia , Tullo Gianluca , Volonnino Gianpietro , Covino Marcello , Franceschi Francesco , La Russa Raffaele
Published: October 2020

Drug abuse (cannabis, cocaine, opiates, and synthetic drugs) is an increasing phenomenon, especially in the younger population, thus leading to more cases of intoxication requiring evaluation in the emergency department and subsequent hospitalization. In 2017, 34.2% of students reported having used an illegal psychoactive substance in their lifetime, while 26% reported having done so over the past year. We made a review about the effectiveness of the role of the temporary observation unit in the emergency department to improve management of acute drugs intoxication. We checked medical literature from the last 10 years (2009–2019). The following electronic databases were systematically searched: MEDLINE-PubMed, Web of Science, Scopus, and the Cochrane Central Register of Controlled Trials. Then, a systematic review was carried out according to the Preferred Reporting Items for Systematic Review standards. Intoxicated patients usually display a favorable medical course, few diagnostic and therapeutic interventions, a short stay in the hospital, and, when hospitalization is needed, semi-intensive therapy is a feasible solution; therefore, intoxicated patients are ideal candidates for a temporary observation unit. The emergency department is very important to manage intoxicated patients; however, the hospitalization of these patients is often not necessary.
Design and Validation of the PercOV-S Questionnaire for Measuring Perceived Obstetric Violence in Nursing, Midwifery and Medical Students
Authors: Mena-Tudela Desirée , Cervera-Gasch Agueda , Alemany-Anchel María José , Andreu-Pejó Laura , González-Chordá Víctor Manuel
Published: October 2020

Background: Obstetric violence could be defined as the dehumanized treatment or abuse of health professionals towards the body or reproductive process of women. Some practices associated with obstetric violence have been routinely standardized and do not include the woman in decision making. This type of violence has consequences for the health of both the mother and the baby and that of the professionals who practice or observed it. Methods: A questionnaire consisting of 33 items that measured perception through a Likert scale was developed. Some sociodemographic variables were collected. The instrument was applied to a sample of nursing, medicine and midwifery students to determine its psychometric properties. Results: The final sample consisted of 153 students. The Kaiser-Meyer-Olkin (p = 0.918) and Barlett tests (p ≤ 0.001) allowed for factor analysis, which explained 54.47% of the variance in two factors called protocolized-visible obstetric violence and non-protocolized-invisible obstetric violence. Conclusions: The PercOV-S (Perception of Obstetric Violence in Students) instrument was validated. The distribution and content of the two factors are closely related to obstetric violence against women. The existence of statistically significant relationships between the sociodemographic variables collected and the global measurements, domains and items of the PercOV-S scale highlight the normalization of obstetric violence as a central factor for future studies.
Expression of EMT-Related Genes in Hybrid E/M Colorectal Cancer Cells Determines Fibroblast Activation and Collagen Remodeling
Authors: Druzhkova Irina , Shirmanova Marina , Ignatova Nadezhda , Dudenkova Varvara , Lukina Maria , Zagaynova Elena , Safina Dina , Kostrov Sergey , Didych Dmitry , Kuzmich Alexey , Sharonov George , Rakitina Olga , Alekseenko Irina , Sverdlov Eugene
Published: October 2020

Collagen, the main non-cellular component of the extracellular matrix (ECM), is profoundly reorganized during tumorigenesis and has a strong impact on tumor behavior. The main source of collagen in tumors is cancer-associated fibroblasts. Cancer cells can also participate in the synthesis of ECM; however, the contribution of both types of cells to collagen rearrangements during the tumor progression is far from being clear. Here, we investigated the processes of collagen biosynthesis and remodeling in parallel with the transcriptome changes during cancer cells and fibroblasts interactions. Combining immunofluorescence, RNA sequencing, and second harmonic generation microscopy, we have explored the relationships between the ratio of epithelial (E) and mesenchymal (M) components of hybrid E/M cancer cells, their ability to activate fibroblasts, and the contributions of both cell types to collagen remodeling. To this end, we studied (i) co-cultures of colorectal cancer cells and normal fibroblasts in a collagen matrix, (ii) patient-derived cancer-associated fibroblasts, and (iii) mouse xenograft models. We found that the activation of normal fibroblasts that form dense collagen networks consisting of large, highly oriented fibers depends on the difference in E/M ratio in the cancer cells. The more-epithelial cells activate the fibroblasts more strongly, which correlates with a dense and highly ordered collagen structure in tumors in vivo. The more-mesenchymal cells activate the fibroblasts to a lesser degree; on the other hand, this cell line has a higher innate collagen remodeling capacity. Normal fibroblasts activated by cancer cells contribute to the organization of the extracellular matrix in a way that is favorable for migratory potency. At the same time, in co-culture with epithelial cancer cells, the contribution of fibroblasts to the reorganization of ECM is more pronounced. Therefore, one can expect that targeting the ability of epithelial cancer cells to activate normal fibroblasts may provide a new anticancer therapeutic strategy.
SATMF Suppresses the Premature Senescence Phenotype of the ATM Loss-of-Function Mutant and Improves Its Fertility in Arabidopsis
Authors: Zhang Yi , Wang Hou-Ling , Gao Yuhan , Guo Hongwei , Li Zhonghai
Published: October 2020

Leaf senescence is the final stage of leaf development. It is accompanied by the remobilization of nutrients from senescent leaves to developing organs. The occurrence of senescence is the consequence of integrating intrinsic and environmental signals. DNA damage triggered by stresses has been regarded as one of the reasons for senescence. To prevent DNA damage, cells have evolved elaborate DNA repair machinery. The ataxia telangiectasia mutated (ATM) functions as the chief transducer of the double-strand breaks (DSBs) signal. Our previous study suggests that ATM functions in lifespan regulation in Arabidopsis. However, ATM regulatory mechanism on plant longevity remains unclear. Here, we performed chemical mutagenesis to identify the components involved in ATM-mediated longevity and obtained three dominant mutants satmf1~3, suppressor of atm in fertility, displaying delayed senescence and restored fertility in comparison with atm mutant. Molecular cloning and functional analysis of SATMF (suppressor of atm in fertility) will help to understand the underlying regulatory mechanism of ATM in plants, and shed light on developing new treatments for the disease Ataxia-telangiectasia.
A Global Proteomic Approach Sheds New Light on Potential Iron-Sulfur Client Proteins of the Chloroplastic Maturation Factor NFU3
Authors: Berger Nathalie , Vignols Florence , Touraine Brigitte , Taupin-Broggini Maël , Rofidal Valérie , Demolombe Vincent , Santoni Véronique , Rouhier Nicolas , Gaymard Frédéric , Dubos Christian
Published: October 2020

Iron-sulfur (Fe-S) proteins play critical functions in plants. Most Fe-S proteins are synthetized in the cytosol as apo-proteins and the subsequent Fe-S cluster incorporation relies on specific protein assembly machineries. They are notably formed by a scaffold complex, which serves for the de novo Fe-S cluster synthesis, and by transfer proteins that insure cluster delivery to apo-targets. However, scarce information is available about the maturation pathways of most plastidial Fe-S proteins and their specificities towards transfer proteins of the associated SUF machinery. To gain more insights into these steps, the expression and protein localization of the NFU1, NFU2, and NFU3 transfer proteins were analyzed in various Arabidopsis thaliana organs and tissues showing quite similar expression patterns. In addition, quantitative proteomic analysis of an nfu3 loss-of-function mutant allowed to propose novel potential client proteins for NFU3 and to show that the protein accumulation profiles and thus metabolic adjustments differ substantially from those established in the nfu2 mutant. By clarifying the respective roles of the three plastidial NFU paralogs, these data allow better delineating the maturation process of plastidial Fe-S proteins.
Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies
Authors: Carbone Annalucia , Linkova Natalia , Polyakova Victoria , Mironova Ekaterina , Hashimova Ulduz , Gadzhiev Ahmed , Safikhanova Khatira , Kvetnaia Tatiana , Krylova Julia , Tarquini Roberto , Mazzoccoli Gianluigi , Kvetnoy Igor
Published: October 2020

Melatonin (MT) and sirtuins (SIRT) are geroprotective molecules that hold back the aging process and the development of age-related diseases, including cardiovascular pathologies. Buccal epithelium (BE) sampling is a non-invasive procedure, yielding highly informative material for evaluating the expression of genes and proteins as well as the synthesis of molecules. Among these, MT and SIRTs are valuable markers of the aging process and age-related pathologies. The purpose of this study was to examine age-related expression patterns of these signaling molecules, in particular MT, SIRT1, SIRT3, and SIRT6 in BE of subjects of different ages with and without arterial hypertension (AH). We used real-time polymerase chain reaction (RT-PCR) and immunofluorescence analysis by confocal microscopy. We found that MT immunofluorescence intensity in BE decreases with aging, more evidently in AH patients. SIRT3 and SIRT6 genes expression and immunofluorescence intensity in BE was decreased in aging controls. In AH patients, SIRT1, SIRT3, and SIRT6 gene expression and immunofluorescence intensity in BE was decreased in relation to age and in comparison with age-matched controls. In conclusion, the evaluation of MT and sirtuins in BE could provide a non-invasive method for appraising the aging process, also when accompanied by AH.
ROS-Dependent ER Stress and Autophagy Mediate the Anti-Tumor Effects of Tributyltin (IV) Ferulate in Colon Cancer Cells
Authors: Celesia Adriana , Morana Ornella , Fiore Tiziana , Pellerito Claudia , D’Anneo Antonella , Lauricella Marianna , Carlisi Daniela , De Blasio Anna , Calvaruso Giuseppe , Giuliano Michela , Emanuele Sonia
Published: October 2020

Organotin compounds represent potential cancer therapeutics due to their pro-apoptotic action. We recently synthesized the novel organotin ferulic acid derivative tributyltin (IV) ferulate (TBT-F) and demonstrated that it displays anti-tumor properties in colon cancer cells related with autophagic cell death. The purpose of the present study was to elucidate the mechanism of TBT-F action in colon cancer cells. We specifically show that TBT-F-dependent autophagy is determined by a rapid generation of reactive oxygen species (ROS) and correlated with endoplasmic reticulum (ER) stress. TBT-F evoked nuclear factor erythroid-2 related factor 2 (Nrf2)-mediated antioxidant response and Nrf2 silencing by RNA interference markedly increased the anti-tumor efficacy of the compound. Moreover, as a consequence of ROS production, TBT-F increased the levels of glucose regulated protein 78 (Grp78) and C/EBP homologous protein (CHOP), two ER stress markers. Interestingly, Grp78 silencing produced significant decreasing effects on the levels of the autophagic proteins p62 and LC3-II, while only p62 decreased in CHOP-silenced cells. Taken together, these results indicate that ROS-dependent ER stress and autophagy play a major role in the TBT-F action mechanism in colon cancer cells and open a new perspective to consider the compound as a potential candidate for colon cancer treatment.
Protective Effects of Colomast®, a New Formulation of Adelmidrol and Sodium Hyaluronate, in a Mouse Model of Acute Restraint Stress
Authors: D’Amico Ramona , Siracusa Rosalba , Fusco Roberta , Cordaro Marika , Genovese Tiziana , Peritore Alessio Filippo , Gugliandolo Enrico , Crupi Rosalia , Impellizzeri Daniela , Cuzzocrea Salvatore , Paola Rosanna Di
Published: October 2020

Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones. Stress is also known to be a risk factor for the development of depression and anxiety. However, until today there are no suitable therapies for treating of stress. The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress. Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization. Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction. Moreover, Colomast® was able to restore tight junction in both ileum and hippocampus and cortex. Additionally, we demonstrated that Colomast® ameliorated depression and anxiety-related behaviours, and modulate inflammatory and apoptosis pathways also in brain after ARS induction. In conclusion, our results suggest Colomast® to be a potential approach to ARS.
DNA Methylation in Nonalcoholic Fatty Liver Disease
Authors: Hyun Jeongeun , Jung Youngmi
Published: October 2020

Nonalcoholic fatty liver disease (NAFLD) is a widespread hepatic disorder in the United States and other Westernized countries. Nonalcoholic steatohepatitis (NASH), an advanced stage of NAFLD, can progress to end-stage liver disease, including cirrhosis and liver cancer. Poor understanding of mechanisms underlying NAFLD progression from simple steatosis to NASH has limited the development of effective therapies and biomarkers. An accumulating body of studies has suggested the importance of DNA methylation, which plays pivotal roles in NAFLD pathogenesis. DNA methylation signatures that can affect gene expression are influenced by environmental and lifestyle experiences such as diet, obesity, and physical activity and are reversible. Hence, DNA methylation signatures and modifiers in NAFLD may provide the basis for developing biomarkers indicating the onset and progression of NAFLD and therapeutics for NAFLD. Herein, we review an update on the recent findings in DNA methylation signatures and their roles in the pathogenesis of NAFLD and broaden people’s perspectives on potential DNA methylation-related treatments and biomarkers for NAFLD.
The Distinct Function and Localization of METTL3/METTL14 and METTL16 Enzymes in Cardiomyocytes
Authors: Arcidiacono Orazio Angelo , Krejčí Jana , Bártová Eva
Published: October 2020

It has become evident that epitranscriptome events, mediated by specific enzymes, regulate gene expression and, subsequently, cell differentiation processes. We show that methyltransferase-like proteins METTL3/METTL14 and N6-adenosine methylation (m6A) in RNAs are homogeneously distributed in embryonic hearts, and histone deacetylase (HDAC) inhibitors valproic acid and Trichostatin A (TSA) up-regulate METTL3/METTL14 proteins. The levels of METTL3 in mouse adult hearts, isolated from male and female animals, were lower in the aorta and pulmonary trunks when compared with atria, but METT14 was up-regulated in the aorta and pulmonary trunk, in comparison with ventriculi. Aging caused METTL3 down-regulation in aorta and atria in male animals. Western blot analysis in differentiated mouse embryonic stem cells (mESCs), containing 10–30 percent of cardiomyocytes, showed METTL3/METTL14 down-regulation, while the differentiation-induced increased level of METTL16 was observed in both wild type (wt) and HDAC1 depleted (dn) cells. In parallel, experimental differentiation in especially HDAC1 wild type cells was accompanied by depletion of m6A in RNA. Immunofluorescence analysis of individual cells revealed the highest density of METTL3/METTL14 in α-actinin positive cardiomyocytes when compared with the other cells in the culture undergoing differentiation. In both wt and HDAC1 dn cells, the amount of METTL16 was also up-regulated in cardiomyocytes when compared to co-cultivated cells. Together, we showed that distinct anatomical regions of the mouse adult hearts are characterized by different levels of METTL3 and METTL14 proteins, which are changed during aging. Experimental cell differentiation was also accompanied by changes in METTL-like proteins and m6A in RNA; in particular, levels and distribution patterns of METTL3/METTL14 proteins were different from the same parameters studied in the case of the METTL16 protein.