bluuebunny/crossref_metadata_2025 huggingface dataset:
FIGURE 3 from PARP Inhibitors Effectively Reduce MAPK Inhibitor Resistant Melanoma Cell Growth and Synergize with MAPK Inhibitors through a Synthetic Lethal Interaction In Vitro and In Vivo
Authors: Fröhlich Lisa Marie , Niessner Heike , Sauer Birgit , Kämereit Sofie , Chatziioannou Eftychia , Riel Simon , Sinnberg Tobias , Schittek Birgit
Published: September 2023

MAPKi resistant cells show decreased ATM expression. A, Immunoblot analysis of A375 R cells treated with 5 μmol/L talazoparib (Tala.) for 24 hours or untreated (Ctr.). Relative protein expression is shown. Actin control was reused from Fig. 2E as the Western blots were performed in the same experiment. B, Immunoblot analysis of chromatin bound fraction of A375 S and R cells after the treatment with 0.005% MMS and 2 μmol/L talazoparib (+) for 6 hours or untreated cells (−). Relative protein expression is shown. C, RT2 Profiler PCR Array Human DNA Repair was performed in A375 S versus R. log2 fold change in the expression of the corresponding genes of R compared with S cells as well as the log10P values of the respective gene expression changes are shown in the volcano plot. Technical triplicates were performed. D, Immunoblot analysis of A375 and Mel1617 S and R cells. Relative protein expression is shown. E, Relative ATM gene expression of A375 and Mel1617 S and R is shown. Unpaired t test was performed to compare the Ctr. with Tala. group. F, IHC of paraffin-embedded tumors of a patient before and after MAPKi resistance stained for ATM. 10X magnification was used. Quantification of the ATM positive cells was performed and % ATM positive cells is shown. G, MUH cell viability assay of HT-144 melanoma cell line treated with different concentrations of talazoparib (Tala.) starting at 50 μmol/L for 72 hours. IC50 level is shown. H, MUH cell viability assay of A375 S and R cells treated with different concentrations of Ku-55933 (Ku.) for 72 hours. Comparison of fits of the nonlinear regression was performed to analyze statistical differences between S and R cells. I, MUH cell viability assay of A375 S cells treated with different concentrations of talazoparib for 72 hours. Cells were additionally treated with a stable concentration of 2 μmol/L Ku-55933 or remained untreated. Comparison of fits of the nonlinear regression was performed to analyze statistical differences between cells treated with Ku-55933 or untreated cells.
Figure S8 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

IFN-γ but not IL-4 is associated with metaplastic pit cell expansion.
Figure S6 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

Spatial profiling reveals enrichment of Muc4 and Areg expression from the luminal surface in the Hp+KRAS+ gastric epithelium.
Figure S5 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

The dysplasia marker gene Trop2 is rarely detected in the central epithelial megacluster, whereas Muc4 and Areg are primarily detected in the central epithelial megacluster.
Figure S4 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

The epithelial subclusters pit_2, pit_6, pit_8 and neck_2 are expanded in Hp+KRAS+ mice.
Figure S3 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

Genes driving the subclustering of the major gastric epithelial cell types.
Figure S2 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

Cell cluster frequencies change according to Hp infection status and induction of constitutively active KRAS.
Figure S14 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

Figure S14. Metaplastic pit cells can be seen in gastric cancer samples.
Figure S13 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

MUC4 expression was not associated with sex or age in our cohort of 47 gastric cancer cases seen in the United States Pacific Northwest.
Figure S11 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

Gastric inflammation is greatest in Hp+KRAS+ mice.
Figure S10 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

Gating strategy for detection of T cell populations by flow cytometry.
FIGURE 5 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

The EGFR ligand amphiregulin is another marker of metaplastic pit cells. A, The heat map shows the normalized expression of the top 25 genes that are most significantly differentially expressed between Muc4-expressing (bottom) and non-expressing (top) metaplastic pit cells (pit_2, pit_6, and pit_8 cells). Genes are ranked from left to right in order of statistical significance. B,Muc4 and Areg coexpression was assessed for each cluster in UMAP #2. Only the five indicated clusters had a Pearson correlation P value < 0.05. The expression of Muc4 and Areg in counts per 10,000 reads is plotted for each cell in the given cluster. Trend lines are plotted and shading indicates the confidence interval of the linear estimation. C, Fluorescent ISH was used to detect Muc4 (green) and Areg (magenta) from representative mice at 12 weeks; DAPI (blue) indicates nuclei and single channel images are shown in grayscale. Scale bars, 100 μm.
FIGURE 3 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Authors: O'Brien Valerie P. , Kang Yuqi , Shenoy Meera K. , Finak Greg , Young William C. , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E. , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M. , Koch Meghan A. , Gottardo Raphael , Salama Nina R.
Published: September 2023

Gastric immunosuppression prevents metaplastic pit cell expansion. A–G, Flow cytometry was performed at 6 weeks using Hp−KRAS+ and Hp+KRAS− mice, and Hp+KRAS+ mice who received either oral dexamethasone (“dex”) starting at 2 weeks or water alone (control, “ctrl”). Graphs indicate the number of live leukocytes isolated from the gastric lamina propria of the indicated mouse groups. CD4−CD8−Ly6C+Ly6G+ neutrophils (A), CD11b variable Ly6C+ MHC II– circulating monocytes (B), CD64+F4/80+ macrophages (C), CD11c high MHC II high conventional dendritic cells (D), CD3+CD8α+CD103+CD44+ effector T cells (E), CD3+CD4+CD103+CD44+ effector T cells (F), CD3+CD4+CD103+CD44+FoxP3+ regulatory T cells (G). N = 2 independent experiments were conducted and data from one experiment is shown. Datapoints represent actual values for each individual mouse, zeroes are plotted at the limit of detection (10 cells), and bars indicate median values. H–J,Hp+KRAS+ mice that were control-treated or dexamethasone-treated are shown. H,Hp was cultured from stomach homogenate supernatants at time of euthanasia. CFU, colony-forming units. Zeroes are plotted at the limit of detection (10 CFU). I and J,Muc4 (brown) was detected by ISH in N = 2 independent experiments with n = 3–6 mice per group. I, Muc4 expression was scored in a blinded fashion. J, Representative images are shown. Scale bars, 100 μm. †, P < 0.10; *, P ≤ 0.05; **, P < 0.01; ns, not significant, Mann–Whitney U test.
Table S4 from Clinical Benefit of Autologous Stem Cell Transplantation for Patients with Multiple Myeloma Achieving Undetectable Minimal Residual Disease after Induction Treatment
Authors: Liu Jiahui , Yan Wenqiang , Fan Huishou , Xu Jingyu , Li Lingna , Du Chenxing , Mao Xuehan , Yan Yuting , Xu Yan , Sui Weiwei , Deng Shuhui , Yi Shuhua , Anderson Kenneth C. , Qiu Lugui , Zou Dehui , An Gang
Published: September 2023

Univariate and multivariate analyses of MRD negativity duration
Table S3 from Clinical Benefit of Autologous Stem Cell Transplantation for Patients with Multiple Myeloma Achieving Undetectable Minimal Residual Disease after Induction Treatment
Authors: Liu Jiahui , Yan Wenqiang , Fan Huishou , Xu Jingyu , Li Lingna , Du Chenxing , Mao Xuehan , Yan Yuting , Xu Yan , Sui Weiwei , Deng Shuhui , Yi Shuhua , Anderson Kenneth C. , Qiu Lugui , Zou Dehui , An Gang
Published: September 2023

Univariate analyses of PFS and OS in 407 transplant-eligible myeloma patients
TABLE 2 from Clinical Benefit of Autologous Stem Cell Transplantation for Patients with Multiple Myeloma Achieving Undetectable Minimal Residual Disease after Induction Treatment
Authors: Liu Jiahui , Yan Wenqiang , Fan Huishou , Xu Jingyu , Li Lingna , Du Chenxing , Mao Xuehan , Yan Yuting , Xu Yan , Sui Weiwei , Deng Shuhui , Yi Shuhua , Anderson Kenneth C. , Qiu Lugui , Zou Dehui , An Gang
Published: September 2023

Multivariate analyses of PFS and OS in 407 patients with transplant-eligible myeloma
Figure S5 from Clinical Benefit of Autologous Stem Cell Transplantation for Patients with Multiple Myeloma Achieving Undetectable Minimal Residual Disease after Induction Treatment
Authors: Liu Jiahui , Yan Wenqiang , Fan Huishou , Xu Jingyu , Li Lingna , Du Chenxing , Mao Xuehan , Yan Yuting , Xu Yan , Sui Weiwei , Deng Shuhui , Yi Shuhua , Anderson Kenneth C. , Qiu Lugui , Zou Dehui , An Gang
Published: September 2023

The impact of continued MRD-negative status (≥2 years) on PFS
Figure S4 from Clinical Benefit of Autologous Stem Cell Transplantation for Patients with Multiple Myeloma Achieving Undetectable Minimal Residual Disease after Induction Treatment
Authors: Liu Jiahui , Yan Wenqiang , Fan Huishou , Xu Jingyu , Li Lingna , Du Chenxing , Mao Xuehan , Yan Yuting , Xu Yan , Sui Weiwei , Deng Shuhui , Yi Shuhua , Anderson Kenneth C. , Qiu Lugui , Zou Dehui , An Gang
Published: September 2023

Subgroup analyses for prognostic impact of ASCT
Figure S3 from Clinical Benefit of Autologous Stem Cell Transplantation for Patients with Multiple Myeloma Achieving Undetectable Minimal Residual Disease after Induction Treatment
Authors: Liu Jiahui , Yan Wenqiang , Fan Huishou , Xu Jingyu , Li Lingna , Du Chenxing , Mao Xuehan , Yan Yuting , Xu Yan , Sui Weiwei , Deng Shuhui , Yi Shuhua , Anderson Kenneth C. , Qiu Lugui , Zou Dehui , An Gang
Published: September 2023

The prognostic impact of ASCT and early MRD status by molecular risk groups
FIGURE 1 from Clinical Benefit of Autologous Stem Cell Transplantation for Patients with Multiple Myeloma Achieving Undetectable Minimal Residual Disease after Induction Treatment
Authors: Liu Jiahui , Yan Wenqiang , Fan Huishou , Xu Jingyu , Li Lingna , Du Chenxing , Mao Xuehan , Yan Yuting , Xu Yan , Sui Weiwei , Deng Shuhui , Yi Shuhua , Anderson Kenneth C. , Qiu Lugui , Zou Dehui , An Gang
Published: September 2023

The impact of ASCT and early MRD status on prognosis. Impact of early MRD-negative status on PFS (A) and impact of early MRD-negative status on OS (B); impact of ASCT by different MRD status on PFS (C); impact of ASCT by different MRD status on OS (D). ASCT: autologous stem-cell transplant; early MRD status: MRD status after induction treatment; NR: not reached; NS: not significant; OS: overall survival; PFS: progression-free survival; *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Pancreatic Ductal Adenocarcinoma Cells Regulate NLRP3 Activation to Generate a Tolerogenic Microenvironment
Authors: Amo-Aparicio Jesus , Dominguez Adrian , Atif Shaikh M. , Dinarello Alberto , Azam Tania , Alula Kibrom M. , Piper Miles , Lieu Christopher H. , Lentz Robert W. , Leal Alexis D. , Bagby Stacey M. , Messersmith Wells A. , Karam Sana D. , Dinarello Charles A. , Pitts Todd M. , Marchetti Carlo
Published: September 2023

Defining feature of pancreatic ductal adenocarcinoma (PDAC) that participates in the high mortality rate and drug resistance is the immune-tolerant microenvironment which enables tumors to progress unabated by adaptive immunity. In this study, we report that PDAC cells release CSF-1 to induce nucleotide-binding domain, leucine-rich containing family, pyrin domain-containing-3 (NLRP3) activation in myeloid cells. Increased NLRP3 expression was found in the pancreas of patients with PDAC when compared with normal pancreas which correlated with the formation of the NLRP3 inflammasome. Using human primary cells and an orthotopic PDAC mouse model, we show that NLRP3 activation is responsible for the maturation and release of the inflammatory cytokine IL1β which selectively drives Th2-type inflammation via COX2/PGE2 induction. As a result of this inflammation, primary tumors were characterized by reduced cytotoxic CD8+ T-cell activation and increased tumor expansion. Genetic deletion and pharmacologic inhibition of NLRP3 enabled the development of Th1 immunity, increased intratumoral levels of IL2, CD8+ T cell–mediated tumor suppression, and ultimately limited tumor growth. In addition, we observed that NLRP3 inhibition in combination with gemcitabine significantly increased the efficacy of the chemotherapy. In conclusion, this study provides a mechanism by which tumor-mediated NLRP3 activation exploits a distinct adaptive immunity response that facilitates tumor escape and progression. Considering the ability to block NLRP3 activity with safe and small orally active molecules, this protein represents a new promising target to improve the limited therapeutic options in PDAC.

Significant:

This study provides novel molecular insights on how PDAC cells exploit NLRP3 activation to suppress CD8 T-cell activation. From a translational perspective, we demonstrate that the combination of gemcitabine with the orally active NLRP3 inhibitor OLT1177 increases the efficacy of monotherapy.
Baseline Assessment of Marine Resources Caught by Fishermen in Selected Coastal Barangays of Cawayan, Masbate, Philippines
Authors: Ibañez, Jr. Roger Y. , Velza Jacob Frederick , Ompoc Janice , Oporto Mark Dave , Mobo Froilan
Published: September 2023

Marine resources have been essential for human consumption and livelihood activities, particularly for small-scale fishermen in the fishing industry. This study employed a descriptive research design, utilizing interviews and structured survey questionnaires for data collection. The findings revealed the presence of 28 fish species caught by the fishermen in Cawayan, including Asian seabass, bisugo, dolphin fish, giant trevally, gold band goatfish, grouper, lattice monocle bream, mackerel tuna, mullet, needlefish, narrow barred, octopus, oxeye scad, pink shrimp, pin spotted spinefoot, pony fish, sardines, sailfish, shark, short mackerel, slip mouth fish, squid, stingray, threadfin emperor, whiting, and yellow stripe scad. The study also identified five shellfish species, namely baler shell, fierce shell, mother-of-pearl shell, scallop, and ark shell. Seaweed species, including sea moss and sea grapes, as well as the presence of blue crab, contributed to the diverse marine ecosystem. Age emerged as a significant factor in fishing, as older fishermen exhibited extensive knowledge and expertise in identifying the caught fish species. Younger individuals, on the other hand, demonstrated physical strength and minimized the risk of work-related injuries. Notably, a significant portion of fishermen (32%) operated boats they did not own, with motorboats being the preferred choice for the majority (93%). Additionally, 72% of respondents indicated that their fishing income covered only their daily expenses, while 57% lacked alternative sources of income during unfavorable weather conditions. The researchers recommend educating and equipping fishermen with the latest fishing knowledge and skills, facilitated by collaborative efforts among the Bureau of Fisheries and Aquatic Resources (BFAR), local government units (LGUs), community extensionists, and extension workers. Sharing sustainable and advanced fishing practices will contribute to the well-being and long-term prosperity of fishing communities.
Home- School Link: Tutor’s Capacity-Building Enhancement Training for effective implementation of Modular distance learning
Authors: Mamolo Jessica Normie F. , Kintanar Fitzgerald C.
Published: September 2023

This study discovers the effectiveness of modular distance learning as an alternative learning modality and proposes a home-school link: a tutor’s capacity-building enhancement training. The study examines the perceived level of competence and tutoring experiences of the parents while administering the self-learning modules (SLM) at home. The study determines the relevance of parents’ tutoring competence and experiences to the learner’s academic achievement, which were identified using a mixed-methods approach through conducting both surveys and interviews. The study used a purposive sampling technique to select the participants. This study reveals that parents are only proficient in administering the module, which demonstrates their need to undergo capacity-enhancement training. Facilitating the SLM emphasizes the significance of the delivery of instruction, employing the student-centered method, appropriate teaching strategies, student motivation, behavior management strategies, and time management. In this connection, the training specifically provides opportunities for the home tutors to acquire the knowledge and skills that they need to deliver a high-quality education to the learners. Moreover, this study reveals the different interventions and challenges encountered while administering modular distance learning. Conducting the teaching-learning process at home can be difficult for home tutors who do not possess the necessary competence to facilitate the SLM, which imposes a risk of producing high-quality outcomes in learners' academic achievement. Hence, it is crucial to implement the home-school link: a tutor’s capacity-building enhancement training to nurture the necessary competences of the home tutors as partners in education. Implementing the enhancement training ensures the quality education our learners wholly deserve.
Effects of labour costs on different branches of service production in Benin
Authors: Houndjo André
Published: September 2023

The study's objective is to evaluate the influence of labour costs on the output of the service sector industries, particularly in the following industries: trade, transport and communication, hotels and restaurants. Inspired by the work of the BLS, we have constructed the labour cost index for the Eurozone, a very high-frequency indicator that will allow us to achieve our objective. The analysis was done over eight years, from the different quarters of 2014 to the third quarter of 2021. Using linear regression, it explores the relationships between the value added of the branches and other variables such as the labour cost index and credit to the economy. To this end, we conducted several tests that allowed us to opt for an error correction model (ECM). The results revealed that labour costs positively influence the firms’ output in the different sectors under study.
Sleep Quality of SHS STEM Students Post-pandemic
Authors: Encabo Justin Jacob , Berja Hazel Marie , Chavez Chrissa Leaj , Dela Pena Sheila Mae , Dormindo Juliana Marie , Lim Jwennyth Wackyn , De Oca Phillip Raymund
Published: September 2023

Sleep is an integral component of maintaining overall health and well-being. Inadequate sleep can result in detrimental effects, both on mental and physiological aspects. While the impact of the COVID-19 pandemic on students' sleep quality was extensively examined, there remains a dearth of literature investigating the prevalence of sleep quality after the shift to a post-pandemic lifestyle. This study utilized a descriptive design and gathered data from 202 randomly selected grades 11 and 12 STEM students. We found that 68.81% of them have poor sleep quality. Data also showed that female students obtained high PSQI scores and had a significantly higher prevalence of poor sleep quality than males. However, there was no significant difference in sleep quality between grade levels. In conclusion, sleep quality among STEM students in the post-pandemic setting is poor, implying the need for further assessment of influencing factors in students' sleep patterns, strategies, and management skills. Furthermore, students should be educated on the importance of sleep and the negative consequences of poor sleep quality on regular activities, mental & physical health, and general well-being.
Memórias dos visitantes do Museu Nacional: lembranças que não se apagam
Authors: Costa Andréa Fernandes , Dahmouche Mônica Santos , Damico José Sergio , Mano Sonia , Cazelli Sibele
Published: September 2023

The Observatório de Museus e Centros de Ciência e Tecnologia (OMCC&T) conducted research entitled Remembrances, Memories, Impacts: What Constitutes the Public of a Science Museum?. A total of 619 forms were filled by visitors of six different museums, of which 87 were drawn to be interviewed about their recollections related to visits to museums. Since over 70% of the memories refer specifically to the National Museum, we chose to focus on these interviewees. With document analysis, which included regiments, institutional reports, photographic collection and journalistic articles, the research investigated the history of the institution, obtaining important elements that help to understand the construction of the relationship of the Museum with its various publics throughout its two thousand years. The historical approach highlights, among other information, the original and current location of the institution and its related problems; the participation of the media in the dissemination process; the configuration of the Museum’s grand collection; the sharing of the territory with a public park and zoo, which conferred on the site characteristics of family and social leisure as well as many visitors throughout the decades. The speeches from the interviewees formed the second pillar of the study, which detailed the emotional bonds of their memories. The reports were characterized by valuing family connections, feelings of happiness, amazement at the magnificence of the palace architecture and the collection, in addition to the deep sadness experienced by the fire that destroyed most of the National Museum property.
BIM como plataforma de participação cidadã
Authors: Pita Juliano Veraldo da Costa , Tramontano Marcelo
Published: September 2023

O presente artigo explora o processo de construção de uma plataforma online para participação comunitária no processo produtivo de obras públicas. O artigo conclui que a plataforma é viável, mas sua implementação demanda uma açào integrada entre diversos atores. Inicialmente são expostos os fundamentos que direcionam esta contrução, seguido pela discussão dos passos do processo, iniciando-se com uma análise de soluçòes existentes, o desenvolvimento de um primeiro protótipo seguido de um ciclo de testes e, finalmente, o desenvolvimento da segunda versão da plataforma. Ao longo do texto são realizadas considerações das decisões e ações em confronto com os fundamentos da pesquisa. Por fim, o artigo encerra-se com conclusòes aprciais da pesquisa ainda em andamento e discute direcionamentos futuros.
Estrutura metodológica para avaliação de desempenho ambiental no processo de projeto de edifícios públicos
Authors: Silva Juliano Libraga da , Passuello Ana Carolina B.
Published: September 2023

O setor da construção possui protagonismo nos impactos ambientais globais e, no Brasil, detém uma parcela significativa representada por edifícios públicos, os quais possuem legislação para adoção de critérios de sustentabilidade em seus projetos, porém limitadas ações práticas. Neste contexto, o presente trabalho propõe uma estrutura metodológica para estimar potenciais impactos ambientais do ciclo de vida de edifícios públicos brasileiros, utilizando a técnica de avaliação do ciclo de vida (ACV) na comparação de soluções construtivas, durante o processo de projeto. O método foi estruturado a partir de revisão sistemática sobre as aplicações da ACV nas fases iniciais de projeto, da análise do processo de projeto de edifícios públicos e por normativas relativas à técnica de ACV. Um estudo de caso foi utilizado para testar o instrumento e os resultados apontam que, com a alteração pontual em um sistema construtivo com impactos relevantes, são obtidas melhorias significativas no desempenho ambiental de todo o ciclo de vida da edificação. Também foi conduzida uma análise de sensibilidade, para verificar a influência dos períodos de vida útil de projeto, considerados para os diferentes cenários avaliados. Foi possível identificar que, com o suporte de especialistas, o método pode contribuir para a inclusão da avaliação de desempenho ambiental na Administração Pública.
Otimização da extração de quantitativos para orçamento de obras por meio de software BIM: uma proposta de matriz de parâmetros
Authors: Santos Ranielle Lopes dos , Campelo Filho Cláudio Roberto , Valério Bianca Maria Vasconcelos
Published: September 2023

No presente artigo, exploramos o processo de construção de uma plataforma online que faz uso das características próprias do BIM (Building Information Modeling) para promover a participação comunitária no processo produtivo de obras públicas, como parte da pesquisa em desenvolvimento pelo grupo Nomads.usp sobre possibilidades de uso de meios digitais para participação. Concluímos que a plataforma é viável, mas sua implementação demanda uma ação integrada entre os diversos atores envolvidos. Apresentamos aqui a análise de soluções e aplicativos existentes, que fundamentou o desenvolvimento de um primeiro protótipo e seu ciclo de testes e, finalmente, o desenvolvimento de uma segunda versão da plataforma. Ao longo do texto, são realizadas considerações sobre as decisões e ações em relação com os fundamentos da pesquisa. Por fim, encerramos o artigo com conclusões parciais da pesquisa, ainda em andamento, e discutimos direcionamentos futuros.
Perfil da produção científica sobre música e idosos: uma revisão bibliométrica
Authors: Mendonça Diogo de Souza , Flores-Gomes Gerson , Silva Lydio Roberto , Oliveira Valdomiro de , Vagetti Gislaine Cristina
Published: September 2023

Contexto: A música tem se mostrado como uma alternativa viável na melhoria e manutenção da qualidade de vida da população idosa. Objetivo: investigar o perfil da produção científica sobre os temas Música e Idosos no período de 2018 e 2022.  Método: foi utilizado o método de revisão do tipo bibliométrica, demonstrando as leis de Lotka, Bradford e Zipf. A busca foi realizada com recorte temporal de 2018 a 2022, com os descritores music, oldert adults, elderly, senior e aged em documentos de acesso aberto da base científica Web of Science. Resultados: A busca na base de dados resultou em 1738 documentos, 1479 artigos completos e 135 revisões da literatura. As principais autoras sobre o tema no período foram Daisy Fancourt (Reino Unido) e Felicity Anne Baker (Austrália). Os Estados Unidos da América foi o país mais produtivo. A principal revista foi a Frontiers in psych com 358 publicações no período. As principais palavras-chave foram Music, Music therapy e Dementia. Conclusões: foi possível perceber a música sendo utilizada para tratamento dos Transtornos Neurocognitivos (leve e maior), do mal de Alzheimer, cognição e memória, e que a musicoterapia estudou tratamentos para a qualidade de vida, saúde e bem-estar, particularmente no tratamento dos sintomas de stress, depressão e ansiedade. A consistência dos resultados permite a indicação de revisões sistemáticas da literatura sobre os tratamentos de desordens neurocognitivas a partir do uso de música para população idosa.
A Importância da autoeficácia na resiliência de idosos cantores de corais durante a pandemia
Authors: Hackenberg Crismarie Casper , Beggiato Sheila Maria Ogasavara , Pastre Taís Glauce Fernandes de Lima , Brun Gilson , Oliveira Valdomiro de , Vagetti Gislaine Cristina
Published: September 2023

Este trabalho tem o objetivo de analisar de que maneira os relatos sobre as fontes de autoeficácia de pessoas idosas ajudam a explicar a autoeficácia geral e fatores associados observados em praticantes do canto coral em modelo remoto durante a pandemia da Covid-19. Realizada nos anos de 2021 e 2022, esta pesquisa durou 1 ano e 5 meses e utilizou a metodologia sequencial mista, integrando resultados quantitativos e qualitativos. Os instrumentos utilizados foram: Entrevista telefônica para avaliação do estado cognitivo (TICS-M), Questionários Sociodemográfico e Socioeconômico da Associação Brasileira de Empresas de Pesquisa (ABEP), Escala de Autoeficácia Geral Percebida (EAGP) e uma entrevista semiestruturada. O estudo quantitativo teve amostra de 140 pessoas idosas, integrantes de 60 corais, em 7 estados do país, que estavam em ação durante a pandemia. No estudo qualitativo foram realizadas entrevistas individuais com 12 coralistas que participaram da amostra quantitativa. Os resultados indicaram que as fontes de autoeficácia confirmaram os altos níveis de autoeficácia geral da amostra. A autoeficácia geral alta da amostra evidencia um grupo motivado e capaz de realizar atividades e desafios musicais, mesmo pela primeira vez atuando em modelo remoto. As fontes de autoeficácia apontaram os domínios da experiência direta e dos indicadores fisiológicos e afetivos como o mais fortalecedores de resilência dos idosos cantores durante a pandemia, sendo o domínio de experiência vicária o mais enfraquecedor.
Comparison of Ambulatory and Home Blood Pressure Variability for Cardiovascular Prognosis and Biomarkers
Authors: Narita Keisuke , Hoshide Satoshi , Kario Kazuomi
Published: September 2023

BACKGROUND:

Although blood pressure variability (BPV) is reported to be associated with cardiovascular prognoses, it has not been established whether ambulatory BPV (ABPV; ie, short-term 24-hour BPV) or home BPV (HBPV; day-to-day BPV) is a superior clinical marker.

METHODS:

We analyzed the associations of ABPV and HBPV with cardiovascular prognoses and biomarkers in 1314 hypertensive outpatients who underwent both home and ambulatory BP measurements in the J-HOP study (Japan Morning-Surge Home Blood Pressure). BPV was evaluated by the SD, coefficient of variation, and average real variability of the patients’ 24-hour ambulatory and home systolic BP values.

RESULTS:

During the median 7.0-year follow-up, 109 cardiovascular events occurred. All SD, coefficient of variation, and average real variability values of the HBPV were significantly associated with cardiovascular risk even after adjusting by average 24-hour ambulatory systolic BP and each ABPV value: 1 SD of hazard ratio (95% CI) for the SD, 1.36 (1.14–1.63); coefficient of variation, 1.38 (1.16–1.66); and average real variability 1.29 (1.10–1.51) of HBPV. The ABPV parameters did not exhibit comparable relationships. The cardiovascular risk spline curves showed a trend toward increased risks with increasing HBPV parameters. There were no differences between ABPV and HBPV in the relationships with B-type natriuretic peptide and the urine albumin-creatine ratio.

CONCLUSIONS:

In this comparative analysis of ambulatory and home BP monitoring values in individuals with hypertension, ABPV was not significantly associated with cardiovascular prognosis adjusted by average BP level, and HBPV was suggested to have modest superiority in predicting cardiovascular prognosis compared with ABPV.
Is Discussing Controversial Issues in RE a Magic Bullet to Promote Social Cohesion? Mapping Opportunities and Challenges Based on Previous Research
Authors: Herbst Jan-Hendrik
Published: September 2023

In the public debate, it is often argued that RE is important because it promotes social cohesion. In the academic debate, however, this position is controversial because, firstly, this goal is associated with a politicization of RE and, secondly, empirical evidence on whether or how RE causes this effect seems to be lacking. In this article, I argue first that cohesion is an adequate subgoal of RE, assuming a social rather than a state-political understanding of cohesion. Second, I refer to international studies on teaching controversial issues (CIs), which show that students can learn participation, tolerance, and common good orientation through high-quality exploration of CIs in class. However, due to methodological difficulties and negative side effects, the discussion of CIs in RE cannot be assumed to be a magic bullet for achieving cohesion. For instance, uncertainties and ambiguities accompany forms of dissent in the classroom, which may lead to authoritarian dispositions and political intolerance. In addition, there are particular challenges for inclusive and especially denominational RE in terms of teachers, issues, students, and external factors.
Young Adults Doing Lived Theology: Three Modes of Theologising
Authors: Laiho Marjukka
Published: September 2023

This study asks: “How do young adults theologise?” It explores creative processes of navigating a gap or a wound experienced by Finnish young adults’ between their personal beliefs and values and the Lutheran religious tradition. The dramatic religious decline and change among young adults has been shown in many studies. Here, the situation is explored through co-theologising with young adults. Based on narrative-discursive analysis of reflexive interviews, the study depicts three modes of theologising: 1) Cognitive theologising explores faith expressed in words, 2) Affective theologising focuses on the power of sensing and feeling, and 3) Practical theologising looks at the human potential and capacity to act. The aim of these modes is not to bridge the gap or heal the wound but to describe and understand it. In the end, these ‘acts of theologising’ are put together, pointing to novel methodological approaches embracing uncertainty and doubt, such as holistic theologising.
Judicial Perspectives on Transforming Equality
Authors: Ma Geoffrey
Published: September 2023

In law, equality is the antithesis of discrimination. As in many other areas of human rights law, however, the right balance very often has to be achieved between competing arguments which are reasonable on their face but which pull in different directions. How does the law achieve this? Constitutional instruments and statutes of course set out some principles and guidelines, but it is largely left to the courts to determine where the balance lies in any given situation. Such balance can only be reached through a principled, not arbitrary, approach that is ultimately based on the concept of dignity. Such principled approach includes the proportionality exercise.
Empathy, a Hallmark of Equality: Shaping Fearlessness Into Transformative Decision-Making and Teaching
Authors: Kapur Naina
Published: September 2023

Whether acting as lawyers, activists, judges, policy makers or academics, there is a void in our equality agendas. This void has enabled the contemporary state to rationalise inequality through the propagation of fear. How can we talk about equality if we do not talk about the current crisis of fear? What shapes our contemporary fears? How might we surpass them? Two contemporary narratives, both from judges, offer the possibility of prevailing over certain kinds of fear to meet equality outcomes. With everything to lose in hostile political circumstances, Justice Attaee’s fearlessness materialises from her lived experiences of inequality from where empathy naturally flows for the benefit of women’s equality against the hostile tide of both Afghan politics and global betrayal. Having never walked ‘in the shoes’ of lgbtqia+ persons, fearlessness for Justice Venkatesh, a deeply conservative judge, is put to the test. Taking on the social fears of himself and others, he cultivates the skill of empathy through the rare and unconventional choice of experiential immersion. Both justices serve as examples for creating inclusive experiential courses on empathy and social context within legal/judicial education institutes and practice, where the lives of those most impacted become central to such learning.
Microverse, Mezzoverse, Macroverse: Protection Against Discrimination in an Artificialised World?
Authors: Muntarbhorn Vitit
Published: September 2023

The article addresses the issue of protection against discrimination in an artificialised world, shaped by automation, algorithms and artificial intelligence (ai) and interlinked with digitalisation. While traditionally advocacy against discrimination was based mainly on the call for specific anti-discrimination laws coupled with other actions, there is a more recent entry point in the form of personal data protection laws. These can help to safeguard privacy in relation to personal data which adds to protection against discrimination. However, the right to privacy and the right to freedom of expression (covering data flows) must be balanced well in the process, and the article refers to recent experiences from various regions. The contribution of different stakeholders is essential. Options include regulation by the State, self-regulation by the business sector, co-regulation between a mix of governmental and business-related cooperation, due diligence measures, technical solutions, consumer-based initiatives, specific attention to vulnerabilities, checks and balances against abuse of power, and demonopolisation.
The Audiovisual Mismatch Negativity in Predictive and Non-Predictive Speech Stimuli in Older Adults With and Without Hearing Loss
Authors: Randazzo Melissa , Smith Paul J. , Priefer Ryan , Senzer Deborah R. , Froud Karen
Published: September 2023

Adults with aging-related hearing loss (ARHL) experience adaptive neural changes to optimize their sensory experiences; for example, enhanced audiovisual (AV) and predictive processing during speech perception. The mismatch negativity (MMN) event-related potential is an index of central auditory processing; however, it has not been explored as an index of AV and predictive processing in adults with ARHL. In a pilot study we examined the AV MMN in two conditions of a passive oddball paradigm — one AV condition in which the visual aspect of the stimulus can predict the auditory percept and one AV control condition in which the visual aspect of the stimulus cannot predict the auditory percept. In adults with ARHL, evoked responses in the AV conditions occurred in the early MMN time window while the older adults with normal hearing showed a later MMN. Findings suggest that adults with ARHL are sensitive to AV incongruity, even when the visual is not predictive of the auditory signal. This suggests that predictive coding for AV speech processing may be heightened in adults with ARHL. This paradigm can be used in future studies to measure treatment related changes, for example via aural rehabilitation, in older adults with ARHL.
Changes in Conversations Across a Century of Popular Cinema
Authors: Cutting James E.
Published: September 2023

Over the course of a century, filmmakers of popular cinema have composed conversations between two characters in several ways. Here, I investigate three. The oldest but initially rare conversational format occurred in silent movies, placing single characters in alternating shots either at midframe or on the same side of the screen. Next, beginning about 1930, movies began to show dyads with the camera looking over the shoulder of one character, and then reversing to show the other over the shoulder of the first. And finally, by the 1950s they increasingly began to place characters in alternating shots on opposite sides of the screen. I assessed the relative frequencies of these three types in over 60,000 shots in 210 movies released from 1915 to 2015. Each of these yoked-shot pairings increased, and by about 1990 they encompassed more than a third of all shots in popular cinema. But their increases were not uniform and their relative prominence has continually changed almost to the present day. A relevant factor is that mean shot durations decreased from the 1960s into the 2000s. Strikingly, once the average shot duration declined to about 5 s by the 1990s, the durations of same-side character pairings became systematically shorter in duration than opposing-side pairings, likely because of constraints of eye movements.
Impact of Westward-Propagating ISO over Mid-High-Latitude Eurasia on SSW during Boreal Winter
Authors: Fan Linjie , Yang Shuangyan
Published: September 2023

In this study, the impact of intraseasonal oscillation (ISO) over mid-high-latitude Eurasia on stratosphere sudden warming (SSW) events during boreal winter is examined based on the NCEP–NCAR reanalysis data. It is found that the dominant ISO periodicity is 10–30 days. By extended empirical orthogonal function, two leading ISO modes with opposite directions of westward and eastward propagation are extracted. Since the eastward-propagating mode was involved by previous studies, this study is focused on the westward-propagating one. As revealed, the westward-propagating ISO propagates from the Canadian Arctic Archipelago to western Europe, which is dominated by waves 1–2. The analysis of lead–lag correlation and phase division indicates that SSW events prefer to occur at the time of lagging the westward-propagating ISO phase 2 by 7–9 days. The diagnosis of the dynamic process of ISO impacting SSW suggests that during the westward journey of the ISO, the corresponding pattern leads to sea ice loss over the Barents–Kara Sea, which emanates upward-propagating planetary waves. At the same time, the ISO circulation expands the easterly anomalies, which weakens westerly flow that favors the planetary waves of wave 1 and wave 2 propagating upward into the stratosphere to affect the onset of SSW. By applying the subseasonal-to-seasonal (S2S) reforecast data from the ECMWF model, the probability density functions of zonal wind anomaly verify the impact of westward-propagating ISO on SSW.

significance statement

The intraseasonal oscillation (ISO) is a vital prediction source for the forecast of extreme weather and climate in both the troposphere and the stratosphere, but the relationship between the mid-high-latitude one and stratospheric extreme weather events lacks research. The purpose of this study is to understand how the ISO over mid-high-latitude Eurasia affects the stratosphere sudden warming (SSW). Our results find that the westward-propagating ISO can impact the onset of SSW events by leading to sea ice loss over the Barents–Kara Sea and the expansion of easterly anomalies. This provides a new clue for the extended-range forecast of SSW.