bluuebunny/crossref_metadata_2025 huggingface dataset:
Pemberdayaan Masyarakat Melalui Kelompok Dukungan Sebaya (KDS) pada Orang Dengan HIV/AIDS di Kota Ternate Provinsi Maluku Utara Tahun 2020
Authors: Supriyatni Nani , Andiani Andiani , Rahayu Agustin , Lestari Tutik
Published: March 2021

Program penanggulangan AIDS di Indonesia mempunyai 4 pilar, yaitu pencegahan (prevention); Perawatan Dukungan dan Pengobatan (PDP); mitigasi dampak berupa dukungan psikososio-ekonomi; serta penciptaan lingkungan yang kondusif (creating enabling environment) (Kemenkes, 2015). Komitmen kuat semua unsur di masyarakat bahwa epidemi HIV/AIDS adalah tanggung jawab bersama pemerintah, non-pemerintah dan masyarakat untuk bahu membahu menekan penyebaran HIV/AIDS. Tujuan : Memberikan dukungan secara psikologis pada teman-teman populasi Kunci dan ODHA melalui Kelompok Dukungan Sebaya/Lembaga Kesejahteraan Sosial Maku Dudara. Metode: dilaksanakan dengan Observasi dan wawancara langsung dengan KDS/LKS Makududara dengan Tahapan yaitu Persiapan, Pengkajian, Perencanaan Alternatif Program, Pemformalisasi Rencana Aksi, Pelaksanaan, Evaluasi. Hasil : Setiap pengurus KDS /LKS Maku Dudara memiliki kemampuan sebagai fasilitator dalam memberikan motivasi kepada sesama teman-teman ODHA bahwa mereka membutuhkan dukungan secara psikologis terhadap kondisi kesehatan saat ini (dengan status HIV). Tahap Pelaksanaan Kegiatan Keikutsertaan teman-teman ODHA terlibat di dalam pengurus KDS/LKS Maku Dudara diharapkan mampu menjalankan program kegiatan yang dapat memberi dukungan kepada teman-teman sesama ODHA. Kerjasama antara pengurus KDS/LKS Maku Dudara bersama teman-teman ODHA dengan fasilitas pelayanan kesehatan selama ini berjalan dengan baik. yaitu : Pendampingan dan Penjangkauan Populasi Kunci dan ODHA, Memfasilitasi Akses Layanan Kesehatan, Bincang Bincang Sehat (BBS), Bakti Sosial, Kunjungan Rumah ODHA, Hari AIDS Sedunia (HAS).
Mulheres na luta: 150 anos em busca de liberdade, igualdade e sororidade
Authors: Farias Ana Luiza Amancio de , Chibante Caroline Gomes , Shubert Carla Oliveira , Silva Cristiane Vanessa Da , Xavier Rozânia Bicego , São Bento Paulo Alexandre De Souza
Published: March 2021

Resenha-se o livro Mulheres na luta: 150 anos em busca de liberdade, igualdade e sororidade (título original: KVINNER I KAMP: 150 ÅRS KAMP FOR FRIHET, LIKHET, SØSTERSKAP!), traduzido do norueguês por Kristin Lie Garrubo e publicado no Brasil, no ano de 2019, pela Editora SCHWARCZ S.A, após acordo com Nordik Literary Agency, França. Propõe-se, no hardbook, com 128 páginas, sob a égide da ludicidade, abordar um século e meio da história de luta das mulheres acerca de liberdade, igualdade e sororidade.
Desafios enfrentados pelos enfermeiros frente à criança com osteogênese imperfeita
Authors: Souza Camila Cruz de , Kropf Rayanne Kelly Pinheiro , Domingues Érika Sousa , Pires Zorahyde Ribeiro , Pinto Ana Cristina Silva
Published: March 2021

Objetivo: identificar os desafios dos enfermeiros na assistência à criança com osteogênese imperfeita. Método: trata-se de um estudo qualitativo, descritivo e exploratório, com 14 enfermeiras plantonistas do complexo pediátrico, que responderam a um questionário. Analisaram-se os dados pela Análise de Conteúdo. Resultados: entendeu-se que o cuidado dessas crianças se torna um desafio, visto que qualquer intervenção inapropriada relacionada à mobilização poderá desencadear complicações devido à fragilidade óssea. Conclusão: aponta-se que o estudo serviu como aprendizado para uma doença não muito conhecida, a qual instiga alguns profissionais de saúde em relação ao seu manejo. Torna-se indispensável, nesse contexto, a sistematização da assistência de Enfermagem aos portadores de OI, já que necessitam de cuidados diferenciados. Descritores: Enfermagem; Ortopedia; Assistência à Saúde; Cuidado da Criança; Enfermeiras Pediátricas; Osteogênese Imperfeita. AbstractObjective: to identify nurses' challenges in the care of children with osteogenesis imperfecta. Method: this is a qualitative, descriptive and exploratory study with 14 on duty nurses in the pediatric complex, who answered a questionnaire. Data was analyzed by Content Analysis. Results: it was understood that the care of these children becomes a challenge, since any inappropriate intervention related to mobilization may trigger complications due to bone fragility. Conclusion: it is pointed out that the study served as learning for a disease that is not well known, which instigates some health professionals regarding its management. In this context, the systematization of nursing care for patients with OI becomes indispensable, as they require special care. Descriptors: Nursing; Orthopedics; Delivery of Health Care; Child Care; Nurses Pediatric; Osteogenesis.ResumenObjetivo: identificar los desafíos de los enfermeros en el cuidado de niños con osteogénesis imperfecta. Método: este es un estudio cualitativo, descriptivo y exploratorio con 14 enfermeras pediátricas en el complejo pediátrico, que respondieron un cuestionario. Los datos fueron analizados por el Análisis de Contenido. Resultados: se entendió que el cuidado de estos niños se convierte en un desafío, ya que cualquier intervención inapropiada relacionada con la movilización puede desencadenar complicaciones debido a la fragilidad ósea. Conclusión: se señala que el estudio sirvió como aprendizaje para una enfermedad no conocida, lo que instiga a algunos profesionales de la salud a tratarla. En este contexto, la sistematización de la atención de enfermería para pacientes con OI se vuelve indispensable, ya que requieren atención diferenciada. Descriptores: Enfermería; Ortopedia; Prestación de Atención de Salud; Cuidado del Niño; Enfermeras Pediátricas; Osteogénesis.
Ritual Pammungkara jodoh Terhadap Pernikahan Dini Di Kabupaten Jeneponto Tahun 2020
Authors: Dewi Dewi , Gobel Fatma Afrianty , Muh. Khidri Alwi  , Een Kurnaesih
Published: March 2021

Latar Belakang: Ritual Pammungkara Jodoh terhadap pernikahan dini merupakan kebiasaan yang dilakukan oleh masyarakat dari zaman dahulu sehingga bersifat  turun temurun yang sampai sekarang masyarakat masih melakukan ritual seperti itu. Pengaruh dari budaya yang ada di Desa Maccini Baji Kabupaten Jeneponto adalah  masalah yang rumit, banyak hal yang berhubungan di sana, oleh karena itu masalah ini sangat perlu perhatian khusus oleh masyarakat yang sadar akan hal itu, karena merugikan remaja dan menghancurkan masa depan remaja, karena selepas itu remaja tersebut akan menikah di bawah umur yang secara fisiologis belum siap, tetapi karena banyak faktor yang mempengaruhi sehingga para orang tua membawa anaknya ke sanro jika sudah berumur 15 tahun, termasuk faktor budaya yang masih erat di desa  dan mereka  mempercayai sanro akan kekuatannya, ditambah lagi faktor ekonomi mereka yang kurang mampu yang hanya rata-rata mengharapkan hasil bumi pertahun, dan pendidikan rendah sehingga pengetahuan mereka kurang akan dampak dan bahayanya remaja yang melakukan  pernikahan dini tetapi masyarakat tidak paham akan hal itu, bahkan mereka menganggap dirinya sudah lepas tanggung jawab jika anak putrinya sudah menikah, kurangnya ilmu agama juga mempengaruhi terjadinya ritual pammungkara jodoh bahkan mereka membenarkan bahwa Sanro mendapatkan kekuatan karena diberikan Rahmat oleh Allah SWT. Tujuan penelitian ini dilakukan untuk menganalisa Ritual Pammungkara Jodoh terhadap pernikahan dini di Kabupaten Jeneponto. Metode: Jenis penelitian adalah penelitian kualitatif dengan pendekatan fenomologi untuk mengeksplorasi mengenai fenomena Ritual Pammungkara Jodoh terhadap pernikahan dini di Kabupaten Jeneponto melalui observasi indepeth dan interview selama penelitian berlangsung. Hasil: Hasil penelitian diperoleh bahwa banyak faktor yang mempengaruhi remaja melakukan Ritual Pammungkara Jodoh terhadap pernikahan dini  di Kabupaten Jeneponto antara lain: Pengaruh budaya yang masih kuat, tingkat ekonomi yang rendah, Pengetahuan rendah, dan kurangnya keyakinan kepada Allah SWT.
Identifikasi Risiko Kecelakan Kerja Terhadap Pekerja Di PT. IKI Makassar Tahun 2020 (Studi Pada Pekerja Proses Marking)
Authors: Riri Rifdah Mufiidah Rusli , A. Fachrin Suharni , Asrina Andi
Published: March 2021

Introduction: Management of occupational health and safety is an integrated effort to manage the risks involved in company activities that can resulted in human injury, damage or disruption to the company's business is divided into three parts, namely Hazard Identification, Risk Assessment and Risk Control (HIRARC) aims to identify potential hazards in the workplace by linking workers, tasks, work equipment and the work environment. The purpose of this study was to identify occupational accident risk then assess the level of occupational accident risk at PT. IKI Makassar in 2020 and how to control it. Methods: The research is qualitative. The approach used in this research is a quasi-qualitative approach. The technique used is observation, in-depth interview) and documentation continuously throughout the study to analyze further about risk identification and control that can be done in the marking process at PT. IKI Makassar. Prior to qualitative research, the risk management process was measured using the HIRARC. The informants in this study were divided into three, that are key informants is the inspector K3, regular informants (part of production process management and knowing the flow of the marking production process), and supporting informants (the workers who work in part of the marking production process) at PT. IKI Makassar. Results: From the results of the study using the HIRARC (Hazard Identification, Risk Assessment and Risk Control) table, it was found that the highest potential hazards and risks were found in the marking process in cutting plates where the most frequent potential hazards such as slipping, falling, slicing and scratching. The conclusion of this research is the Potential dangers contained in the marking process at PT. Makassar IKI covers mechanical hazards in the marking area, ie pinched, dropped, scratched, sliced, knocked and crushed.
Penegakan Hukum Terhadap Penyalahgunaan Narkotika: Studi Kota Makassar
Authors: Kamarudin Muhammad Faisal , Agis Abdul , Fadil Ahmad
Published: March 2021

Tujuan penelitian: (1) Untuk mengetahui dan menganalisis tentang penegakan hukum terhadap penyalahgunaan narkotika di kota makassar; (2) Untuk mengetahui dan menganalisis tentang faktor-faktor apa saja yang mempengaruhi penegakan hukum terhadap penyalahgunaan narkotika di kota makassar. Metode penelitian yang digunakan pada penulisan ini adalah penelitian empiris. Kesimpulan penelitian: (1) Penegakan hukum terhadap penyalahgunaan narkotika di kota makassar dilaksanakan dengan menggunakan sarana penal dan non penal. (2) Faktor yang mempengaruhi penegakan hukum terhadap penyalahgunaan narkotika di kota makassar adalah Faktor kesulitan terhadap saksi yang masih menghambat kepolisian dalam hal penanganan terhadap penyalahgunaan narkotika di masyarakat; Faktor kemampuan finansial menjadi salah satu faktor yang mempengaruhi penegakan hukum terhadap penyalahgunaan narkotika; Faktor mental atitude para penegak hukum juga mempengaruhi penegakan hukum terhadap penyalahgunaan narkotika karena faktanya di masyarakat ada yang beranggapan atitude para penegak hukum dalam hal pemberantasan tindak pidana narkotika terkesan tebang pilih.

Research purposes: (1) To find out and analyze the law enforcement of narcotics abuse in Makassar; (2) To find out and analyze what factors influence the law enforcement of narcotics abuse in Makassar. The research method used in this paper is empirical research. Research conclusions: (1) Law enforcement against narcotics misuse in the city of Makassar is carried out by using penal and non-penal means. (2) Factors that influence law enforcement against narcotics abuse in the city of Makassar are the difficulties for witnesses who are still hampering the police in handling narcotics abuse in the community; The financial capability factor is one of the factors that influence law enforcement against narcotics abuse; Mental factor atitude of law enforcement also influences law enforcement against narcotics abuse because the fact is that there are those in the community who assume that the attitudes of law enforcers in the eradication of narcotics crime seem to be selective.
Perlindungan Hukum Hak-Hak Istri Pasca Perceraian
Authors: Nasriah Nasriah , Busthami Dachran S , Baharuddin Hamza
Published: March 2021

Penelitian bertujuan mengetahui bentuk perlindungan Terhadap Istri Melalui putusan pengadilan di Pengadilan Agama Belopa dan mengetahui pelaksanaan isi putusan hakim Pengadilan Agama Belopa tentang pemberian nafkah mantan istri akibat cerai talak. Metode yang digunakan adalah metode penelitian yuridis-sosiologis. Hasil penelitian menunjukkan bahwa Penerapan bentuk perlindungan hukum terhadap istri yang diberikan melalui putusan pengadilan yaitu berupa pemberian nafkah lampau, nafkah mut’ah, nafkah iddah, dengan cara pembebanan kepada bekas suami. Dan Pelaksanaan pemberian nafkah mantan istri akibat cerai talak dilaksanakan setelah suami membacakan ikrar talak atau setelah putusan berkekuatan hukum tetap. Namun dalam prakteknya, banyak suami yang tidak mau membayarkan nafkah mantan istri di persidangan, sehingga hakim memberikan kebijakan dengan memerintahkan suami untuk membayarkan mut’ah, nafkah iddah, dan nafkah madhiyah sebelum pembacaan ikrar talak atau menunda sidang penyaksian ikrar talak bagi suami yang ingkar terhadap kewajibannya. Kebijakan tersebut dilakukan untuk memberikan perlindungan hak-hak mantan istri dan memberikan keadilan bagi istri yang ditalak oleh suaminya.

This study aims to determine the form of protection for wives through court decisions at the Belopa Religious Court and to find out the implementation of the contents of the Belopa Religious Court judge's decision regarding providing a living for ex-wives due to divorce. The method used is a sociological-juridical research method. The results showed that the application of the form of legal protection to the wife that was given through a court decision was in the form of giving a past income, living a mut'ah, iddah income, by means of imposition to the ex-husband. And the provision of income for the ex-wife due to divorce is carried out after the husband has read the divorce vow or after the decision has permanent legal force. However, in practice, many husbands do not want to pay for their ex-wives in court, so the judge provides a policy by ordering their husbands to pay mut'ah, iddah livelihoods, and madhiyah livelihoods before reading the divorce vows or postpone the hearing of the divorce vows for husbands who renounce obligation. This policy was carried out to protect the rights of the ex-wife and to provide justice for the wife who was bullied by her husband.
Implementasi Perlindungan Hukum Terhadap Anak Melalui Diversi Oleh Jaksa Penuntut Umum
Authors: Ramlah Ramlah , Fahmal A Muin , Nuh Muhammad Syarief
Published: March 2021

Penelitian bertujuan menganalisis implementasi perlindungan hukum terhadap anak melalui diversi oleh jaksa penuntut umum. Tipe penelitian ini adalah yuridis empiris, data yang diperoleh penulis dari studi dokumen maupun wawancara dengan pihak yang berkepentingan dalam hal ini pihak Kejaksaan Negeri Makassar, kemudian dilakukan analisis deskriptif kuantitatif. Hasil Penelitian implementasi upaya diversi yang dilakukan oleh Jaksa Penuntut Umum terhadap perkara tindak pidana anak di Kejaksaan Negeri Makassar telah terlaksana dengan baik dari segi prosedural. Pelaksanaan yang dilakukan oleh aparat penegak telah sesuai dengan undang-undang sistem peradilan anak serta Peraturan Jaksa Agung Republik Indonesia. Namun kenyataannya pada pelaksanaan diversi di Kejaksaan Negeri Makassar seringkali dijumpai anak yang mengulangi tindak pidana sehingga Jaksa Penuntut Umum tidak mengupayakan diversi melainkan melimpahkan berkas perkara pada Pengadilan Negeri Makassar, yang mana telah sesuai dengan unsur-unsur pelaksanaan diversi itu sendiri sebagaimana yang diatur dalam Pasal 7 Ayat 2 Undang-Undang Nomor 11 Tahun 2012 Tentang Sistem Peradilan Anak. Faktor yang menghambat terhadap implementasi perlindungan hukum terhadap anak melalui diversi oleh jaksa penuntut umum antara lain: substansi hukum, struktur hukum dan budaya hukum.

This study aims to analyze the implementation of legal protection against children through diversion by the public prosecutor. This type of research is juridical empirical, the data obtained by the author from document studies and interviews with interested parties, in this case the Makassar District Attorney, then carried out a quantitative descriptive analysis. The results of the research on the implementation of diversion efforts carried out by the public prosecutor on child criminal cases at the Makassar District Prosecutor's Office have been carried out well from a procedural perspective. Implementation carried out by the enforcement apparatus is in accordance with the law on the juvenile justice system and the Attorney General's Regulation of the Republic of Indonesia. However, in reality in the implementation of diversion at the Makassar District Prosecutor's Office, there are often children who repeat the crime so that the Public Prosecutor does not seek diversion but instead delegates the case file to the Makassar District Court, which is in accordance with the elements of the diversion implementation itself as regulated in Article 7. Paragraph 2 of Law Number 11 Year 2012 concerning the Juvenile Justice System. Factors that hinder the implementation of legal protection against children through diversion by the public prosecutor include: legal substance, legal structure and legal culture.
Fungsi Kepolisian Dalam Penyidikan Tindak Pidana Korupsi Pengadaan Barang Dan Jasa
Authors: Ridwan Ridwan , Thalib Hambali , Djanggih Hardianto
Published: March 2021

Penelitian ini bertujuan Untuk menganalisis dan menjelaskan peran kepolisian dalam penyidikan tindak pidana korupsi pengadaan barang dan jasa di wilayah hukum Polres Pelabuhan Makassar. Untuk menganalisis dan menjelaskan faktor-faktor yang mempengaruhi fungsi kepolisian dalam penyidikan tindak pidana korupsi pengadaan barang dan jasa di wilayah Polres Pelabuhan Makassar. Berdasarkan analisis terhadap data dan fakta penulis berkesimpulan bahwa Fungsi kepolisian dalam penyidikan tindak pidana korupsi pengadaan barang dan jasa di wilayah hukum Polres Pelabuhan Makassar terimplementasi. Faktor-faktor yang mempengaruhi fungsi kepolisian dalam penyidikan tindak pidana korupsi pengadaan barang dan jasa di wilayah Polres Pelabuhan Makassar adalah struktur hukum dan budaya hukum masyarakat.

The aims are: To analyze and explain the role of police corruption investigation procurement of goods and services in the jurisdiction of port resort police makassar; To analyze and explained the factors influencing the police investigation of corruption procurement of goods and services in the port resort police makassar. The result of the research, The police investigation of corruption procurement of goods and services in the jurisdiction of makassar implemented port police; Affects functioning of police in the corruption investigation procurement of goods and services in the makassar port resort police structure culture is law and the laws of society.
Pengaruh Pengembangan Sumber Daya Aparatur Terhadap Kinerja Aparatur Biro Hukum dan Ham Setda Provinsi Sulawesi Selatan
Authors: Saharuddin S , Jafar Sahari
Published: March 2021

The results of this study indicate that all variables, namely: education, training and career development have a positive and significant influence simultaneously on the performance of Apparatus Bureau of Law and Ham Setda South Sulawesi Province. Partially found that career development factor that has the most dominant significant influence on the performance of Apparatus Bureau of Law Dan Ham Setda South Sulawesi Province.
Teaching an International Bestseller: Reflections on Encouraging Caring Global Citizenship
Authors: Davidova Evguenia
Published: March 2021

This article discusses an integrative model of gradual scaffolding of curricular and pedagogical strategies, based on the theoretical framework of a “caring global citizenship.” It provides concrete practices for internationalization of general education that could be implemented in a wide variety of institutional settings. Such a model addresses ethnocentrism by expanding students' knowledge and perspectives on societies influenced by American politics, and fosters empathy as both value and skill.
Linkages Between Grade Point Average and Student Ratings
Authors: Richardson Robert D , Williams Robert L.
Published: March 2021

In order to better understand the potential influence of high school students’ grades on how they rate their teachers and schools, we explored the relationship between student grade point average and student ratings of teacher and school effectiveness in 370 classes taught by 230 instructors with over 6,000 students in grades 9–12 in an Intermountain West school district. Teachers were evaluated with an 18-item student survey. Students also rated their schools with six additional items. The performance measure was the grade point average (GPA) for the quarter in which students evaluated their teachers and schools. ANOVAs showed that both year in school and GPA were positively linked with ratings of teacher effectiveness and ratings of school experiences. Seniors were more positively disposed toward their teachers and school experiences than students at lower grade levels. Most of the ratings of specific items on the teacher effectiveness and school-experience scales were significantly, though not strongly, correlated with GPAs. We discuss implications and suggestions for future research.
Moderne Schauspieldidaktik im Grenzbereich der Psychotherapie
Authors: Konradi Elias
Published: March 2021

Whether we like it or not, in human interaction we all hide behind certain “masks”. These masks are always present and supposed to protect us. Actors have to overcome this protective mechanism in order to fill characters with real life. This is where the “hot chair” method gets used.

For this book five acting students from Alanus University were asked about their respective experiences with the “hot chair” method. Their reports provide information on the opportunities and risks of this method. Five potential types of risks and opportunities were identified. The book closes with recommendations for active users and thus offers an asset for every acting didactic or theater teacher.
Reformbedürftigkeit des Fortpflanzungsmedizinrechts
Authors: Merrem Marie-Theres
Published: March 2021

In Germany, technologies and procedures that serve to induce pregnancy are strongly regulated or forbidden by the embryo protection law. Marie-Theres Merrem initially takes a critical look at the current legal situation, with particular emphasis on egg, semen and embryo donation, elective single embryo transfer, gestational surrogacy, post-mortem fertilization, and egg freezing. Based on this, implementation proposals for a renewed Reproductive Medicine Act are presented, which takes current scientific knowledge, social values and the needs of those affected into account.
La iconografía de Cristo oculto y la «Fe oscura» de S. Juan de La Cruz.
Authors: Cuesta Garcia de Leonardo Maria Josefa
Published: March 2021

Al poco de morir Juan de la Cruz, en los inicios del siglo XVII, se intenta su canonización. Para ello se buscan imágenes que aludan al mismo, con carácter milagroso y haciendo referencia a su doctrina.  Remitiéndonos a distintos textos,  veremos cómo sus seguidores construyen una imagen inédita para materializar un concepto, el de la «Fe oscura», usando el término dado por el creador del mismo, Juan de la Cruz.

PALABRAS CLAVE: 

S. Juan de la Cruz, Cristo oculto, Fe oscura, C. Boel, K. Knyf, C. de Mallery, Carmelitas descalzos, Siglos XVI-XVII.
Series de gestión ISO: más allá de sus objetos
Authors: Garcia Alsina Montserrat
Published: March 2021

Las series de normas de gestión ISO recogen diversos requerimientos para la correcta gestión de aspectos como la calidad (9000), el medio ambiente (14000), la seguridad de la información (27000) o los documentos (30300). Además, están dirigidas a la excelencia y mejora continúa. Pero si nos paramos a pensar, estos sistemas de gestión van más allá, y su operativa necesita de la implicación de metodologías y conocimientos reunidos en disciplinas hasta ahora descuidadas en muchas de las organizaciones: la gestión documental y la inteligencia competitiva.
Estética Instagram: de fotografías, filtros y 'punctum'
Authors: San Cornelio Gemma
Published: March 2021

Domingo por la tarde, mientras juega la selección de fútbol, salgo a dar un paseo y me siento en un banco en una avenida de Barcelona mientras contemplo a mi lado a dos chicos haciendo toques con un balón. De repente, observo en el gemelo de uno de los chicos un tatuaje de una cámara réflex y pienso: ésta sería una foto genial para publicarla en Instagram. Saco mi móvil, activo la aplicación y trato de encuadrar…
Influence of water availability and temperature on estimates of microbial extracellular enzyme activity
Authors: Gomez Enrique J. , Delgado Jose A. , Gonzalez Juan M.
Published: March 2021

Soils are highly heterogeneous and support highly diverse microbial communities. Microbial extracellular enzymes breakdown complex polymers into small assimilable molecules representing the limiting step of soil organic matter mineralization. This process occurs on to soil particles although currently it is typically estimated in laboratory aqueous solutions. Herein, estimates of microbial extracellular enzyme activity were obtained over a broad range of temperatures and water availabilities frequently observed at soil upper layers. A Pseudomonas strain presented optimum extracellular enzyme activities at high water activity whereas a desiccation resistant bacterium (Deinococcus) and a soil thermophilic isolate (Parageobacillus) showed optimum extracellular enzyme activity under dried (i.e., water activities ranging 0.5–0.8) rather that wet conditions. Different unamended soils presented a distinctive response of extracellular enzyme activity as a function of temperature and water availability. This study presents a procedure to obtain realistic estimates of microbial extracellular enzyme activity under natural soil conditions of extreme water availability and temperature. Improving estimates of microbial extracellular enzyme activity contribute to better understand the role of microorganisms in soils.
Contribuições para Indicação Geográfica (IG): considerações sobre Itororó – BA como uma potencial IG para Carne de Sol
Authors: Santos Joelito Cruz , Carvalho dos Santos Wagna Piler
Published: March 2021

Este trabalho tratou de identificar as características associadas da carne de sol produzida no município de Itororó, Bahia, Brasil, com o objetivo de demonstrar a potencialidade de registro de Indicação Geográfica (IG) para o território abrangido pelo município. A pesquisa foi dividida em duas partes, diferenciação do produto e a determinação de área de indicação geográfica. Feita a revisão bibliográfica, foram comparadas as ações, as características existentes no município em questão, assim como as características em relação ao produto. Conclui-se que há a potencialidade de o território do município de Itororó, BA obter a proteção industrial pela Indicação Geográfica para o produto alimentício "carne do sol".
Disseminação da Propriedade Intelectual como Estratégia para Políticas de Ciência, Tecnologia e Inovação: o caso do Sistema Pernambucano de Inovação (SPIn)
Authors: Bemfica Eduardo Andrade , Cavalcanti André Marques
Published: March 2021

Este trabalho tem como objetivo propor ações de disseminação do conhecimento em Propriedade Intelectual (PI) para as instituições que compõem o Sistema Pernambucano de Inovação (SPIn) mapeadas no documento do governo do estado intitulado Estratégia de Ciência, Tecnologia e Inovação para Pernambuco 2017-2022 (ECT&I-PE). A metodologia utilizada foi uma abordagem qualitativa, de caráter descritivo, baseada em análise da literatura correspondente ao tema e em pesquisa documental. No referencial teórico, houve uma exposição sobre o papel dos Sistemas Regionais de Inovação (SRI) e a importância da disseminação da Propriedade Intelectual (PI) como estratégia dentro do processo de inovação. Como resultado, são apresentadas propostas com linhas de ações de disseminação do conhecimento da PI agrupadas aos seis eixos estratégicos do documento, a fim de nortear e de impactar as políticas públicas de inovação para a área de Ciência, Tecnologia e Inovação do estado.
Prospecção Tecnológica sobre Biomarcadores Relacionados com Diagnóstico de Doenças Psiquiátricas
Authors: Santos Ana Caroline Melo dos , Silva Denise Macedo da , Santos Bruna Brandão dos , Farias Karol Fireman de , Figueiredo Elaine Virginia Martins de Souza
Published: March 2021

Os transtornos mentais configuram-se como uma doença que possui características psicológicas associadas ao comprometimento funcional resultante de disfunção biopsicossocial. Métodos de detecção com a utilização de biomarcadores que permitam e que gerem resultados rápidos, seguros e reprodutíveis são necessários para auxiliar os profissionais de saúde na avaliação e no monitoramento das doenças psiquiátricas. Foram incluídas as descrições das patentes que abordassem o desenvolvimento de um biomarcador aplicado para o diagnóstico de doenças psiquiátricas, posteriormente foram excluídas as patentes repetidas na mesma base, patentes de Adição e que abordassem métodos de imagem e com enfoque em doenças neurológicas. A prospecção mostrou que a maioria dos depósitos de patentes envolvendo tecnologias para comorbidades psiquiátricas, teve um aumento crescente nos últimos anos principalmente a partir de 2012, tendo os Estados Unidos como maior país depositante e acúmulo de inventores, com pouco desenvolvimento tecnológico sobre a temática no Brasil.
Indicações Geográficas: agregação de valor da renda de bilro de Saubara
Authors: Conceição Valdir Silva , Rocha Angela Machado
Published: March 2021

O objetivo deste trabalho é analisar a possibilidade de geração de valor da renda de bilro de Saubara por meio do registro de Indicação Geográfica (IG), verificando a evolução da importância dada ao tema, e debater a estratégica da IG como um dos instrumentos geradores de valor e os seus aspectos mercadológicos, o que proporciona um diferencial competitivo para o produto no mercado nacional e internacional. A implantação de uma IG valoriza o produto e/ou serviço, o patrimônio cultural, o território, a diversidade, a identidade e a tradição da cultura local, a propriedade intelectual e a ancestralidade com o saber-fazer, padroniza o produto, garante a qualidade, capacita a mão de obra entre outros benefícios para a comunidade. A pesquisa vai ser do tipo empírico e de caráter qualitativo, utilizando como método a pesquisa bibliográfica, a revisão da literatura sobre a renda de bilro, o município de Saubara e a IG e os benefícios agregados advindos do registro de uma IG para as rendeiras e para o território no qual se encontra inserido.
Non‐processive transcription of poly[d(A—T)] by wheat germ RNA polymerase II
Authors: Durand Robert , Job Claudette , Teissère Marcel , Job Dominique
Published: July 2002

RNA product distribution obtained during the transcription of poly[d(A—T)] by wheat germ RNA polymerase IIA under various experimental conditions was analyzed by high resolution polyacrylamide gel electrophoresis. Poly[r(A—U)] synthesis proceeded as if wheat germ RNA polymerase II was a non‐processive enzyme: a ladder of RNA products of increasing lengths was obtained, which apparently, terminated at every other nucleotide. RNA release was not dependent upon nucleoside triphosphate substrate concentrations. A likely explanation would be that ternary complexes enzyme: DNA: RNA were very much unstable; moreover, oligonucleotides released were not re‐used for further elongation by the enzyme.
Heparin solubilizes asymmetric acetylcholinesterase from rat neuromuscular junction
Authors: Torres Juan Carlos , Inestrosa Nibaldo C.
Published: July 2002

We are interested in the factors involved in the anchorage of acetylcholinesterase (AChE) to the synaptic basal lamina. Here, we report studies showing that heparin, a sulfated glycosaminoglycan, specifically solubilized AChE from endplate regions of rat diaphragm muscle. Of the several molecular forms of AChE present in that region, heparin only released the asymmetric A12 and A8 forms of the enzyme. Our results strongly support the involvement of heparin‐like macromolecules in the in vivo immobilization of the collagen‐tailed forms of AChE to the basal lamina of the neuromuscular junction.
Occurrence of two distinct citrate synthases in a mutant of Pseudomonas aeruginosa and their growth‐dependent variation
Authors: Solomon M. , Weitzman P.D.J.
Published: July 2002

Two distinct citrate synthases have been found in a mutant of Pseudomonas aeruginosa — a ‘large’ form (M
r 2̃50 000) and a ‘small’ form (M
r 1̃00 000). In common with the citrate synthase of wild‐type Ps. aeruginosa and other aerobic Gram‐negative bacteria, the ‘large’ enzyme is regulated by both NADH and AMP, whereas the ‘small’ enzyme is insensitive to these effectors. The relative proportions of the two citrate synthases vary with the stage of growth of the bacterial culture. The ‘large’ form predominates in logarithmic phase, whereas the ‘small’ form is the major component in stationary phase. The two forms do not appear to be interconvertible by a simple dissociation—association mechanism.
The mitochondrial phosphate carrier reconstituted in liposomes is inhibited by doxorubicin
Authors: Cheneval D. , Müller M. , Carafoli E.
Published: July 2002

The phosphate carrier has been isolated from beef heart mitochondria in the presence of cardiolipin and reconstituted in asolectin vesicles. It has been found that 100 μM doxorubicin and 100 μM Br‐daunomycin inhibit the unidirectional phosphate uptake in the reconstituted liposomes to the same extent as N‐ethylmaleimide. The inhibition by Br‐daunomycin is not due to covalent interaction with the carrier. The specific interaction between doxorubicin and cardiolipin is responsible for the inhibition of the phosphate carrier. Br‐daunomycin interacts with 3 mitochondrial proteins of apparent M
r ∼45 000, ∼35 000 and ∼30 000.
Essential protein factors for polyprenyl pyrophosphate synthetases
Authors: Fujii H. , Koyama T. , Ogura K.
Published: July 2002

Heptaprenyl pyrophosphate synthetase from Bacillus subtilis was dissociated into two essential components, none of which had catalytic activity alone. The enzyme activity was restored when the two components were combined with each other. Both fractions, designated components I and II in the order of their elution from DEAE‐Sephadex, appeared to be proteins of M
r 30 000. Component I was much more stable than component II which was easily destroyed by relatively mild heat treatment. Neither was inter‐changeable with any of the essential components of hexaprenyl pyrophosphate synthetase of Micrococcus luteus B‐P 26.
A product‐regulated fructose 2,6‐bisphosphatase occurs in green leaves
Authors: Cséke Csaba , Stitt Mark , Balogh Árpád , Buchanan Bob B.
Published: July 2002

An enzyme catalyzing the hydrolytic conversion of fructose 2,6‐bisphosphate (Fru‐2,6‐P2) to fructose 6‐phosphate (Fru‐6‐P) and Pi has been identified and purified from plants, specifically the cytosolic fraction of spinach leaf parenchyma cells. Partially purified preparations of the enzyme, designated fructose 2,6‐bisphosphatase (Fru‐2,6‐P2ase), were inhibited by products of the reaction (i.e., Pi and Fru‐6‐P) but showed no response to a protein phosphorylation system known to inhibit the corresponding enzyme in mammalian cells. Fru‐2,6‐P2ase co‐purified with fructose 6‐phosphate,2‐kinase, the enzyme catalyzing the synthesis of Fru‐2,6‐P2. The observed pattern of regulation of the enzymes functional in the synthesis and breakdown of Fru‐2,6‐P2 reinforces the conclusion that chloroplasts play a role in controlling cytosolic carbon processing in leaves.
Some properties of adenylate cyclase which might be important for memory formation
Authors: Dudai Yadin
Published: July 2002

Analysis of the biochemical defects detected in a Drosophila memory mutant permits dissection of properties of adenylate cyclase which may play a role in elementary memory mechanisms. Of special relevance appear to be those properties which are related to the intracellular regulation of the enzyme.
Involvement of lysine residues in the binding of ovine prolactin and human growth hormone to lactogenic receptors
Authors: de la Llosa P. , Chêne N. , Martal J.
Published: July 2002

The lactogenic activity (L.A.) of oPRL and hGH derivatives obtained by chemical modifications of lysine residues was studied by radioreceptor assay. Control treatment with borohydride had a slight effect on the L.A. of hGH but drastically reduced the oPRL activity; this latter was preserved in the presence of iodoacetamide. Methylation, ethylation, guanidination and acetimidination affected the L.A. of both hormones as a function of the degree of modification. The structure‐binding relationships to the lactogenic receptors are discussed, suggesting that the lysine or arginine residues in homologous positions 42, 51, 73, 128, 146 of oPRL and 47, 50, 73, 128, 147 of hGH might be particularly involved.
Primary structure of toxin IV of Leiurus quinquestriatus quinquestriatus
Authors: Kopeyan Charles , Martinez Gérard , Rochat Hervé
Published: July 2002

We report the sequence of toxin IV of Leiurus quinquestriatus quinquestriatus, one of the five toxins lethal for the mouse purified from the venom of this scorpion. Automatic sequencing of the S‐carboxymethylated protein and derived peptides obtained by action of the Staphylococcus aureus protease allowed us to work out the complete sequence of 65 residues. The toxin which was found to be blocked at the C‐terminal end has an extra residue at the N‐terminus as compared with other toxins from Asian and African scorpions. Moreover, toxin IV must be classified as an α‐scorpion toxin since it was shown to displace competitively 125I‐toxin II of Androctonus australis Hector (125I‐AaH II) from its binding site on rat brain synaptosomes. Nevertheless, it cannot be classified in one of the groups of scorpion toxins previously defined on the level of their antigenic properties. Again the classifications of scorpion toxins based on sequence homologies or antigenic properties support one another.
A novel structural transition in poly(dG‐Me5dC):Z⇄B⇄Z
Authors: Latha P.K. , Brahmachari Samir K.
Published: July 2002

Poly(dG‐Me5dC) is known to exhibit a B→Z transition in the presence of very high concentrations of NaCl. For the first time, we report the presence of a Z‐structure in sodium concentrations as low as 0.5 mM. A novel Z⇄B⇄Z transition is observed as the salt concentration is gradually increased. The role of water structure in B to Z transitions is discussed.
High‐affinity association and degradation of 125I‐labelled low density lipoproteins by human hepatocytes in primary culture
Authors: Kosykh V.A. , Preobrazhensky S.N. , Ivanov V.O. , Tsibulsky V.P. , Repin V.S. , Smirnov V.N.
Published: July 2002

Human hepatocyte
LDL
Cell association
Degradation
High affinity association
[Ser7]neurotensin: isolation from guinea pig intestine
Authors: Shaw Christopher , Thim Lars , Conlon J.Michael
Published: July 2002

Using three antisera to neurotensin of defined regional specificity, a novel neurotensin has been identified in extracts of guinea pig brain and small intestine. The primary structure of the peptide was established as: pGlu Leu Tyr GIu Asn Lys Ser Arg Arg Pro Tyr He Leu. Guinea pig neurotensin differs from bovine neurotensin by substitution of a prolyl residue by a seryl residue at position 7. Synthetic [Ser7]neurotensin showed identical Chromatographie and immunochemical properties to guinea pig neurotensin. This difference in primary structure may account for some of the anomalous pharmacological effects of bovine neurotensin on guinea pig tissues.
Redox studies of the tetraheme cytochrome c3 isolated from the propionate‐oxidizing, sulfate‐reducing bacterium Desulfobulbus elongatus
Authors: Samain Eric , Albagnac Guy , Legall Jean
Published: July 2002

A tetraheme cytochrome c
3 was purified from extracts of Desulfobulbus elongatus. Redox potentiometry studies indicated that one of the four hemes has a uniquely high E
m value (−30 mV), whereas the other three have a lower redox potential with E
m values of −165 mV. In the presence of a membrane preparation from Db. elongatus the cytochrome c
3 is partially reduced (23% at pH 7.6) by succinate. The physiological significance of the high redox potential of this cytochrome c
3 is discussed as a function of the propionate metabolism of Desulfobulbus which is known to proceed via the succinate pathway.
Isolation from chicken antrum, and primary amino acid sequence of a novel 36‐residue peptide of the gastrin/CCK family
Authors: Dimaline R. , Young J. , Gregory H.
Published: July 2002

A peptide that cross‐reacted with C‐terminal gastrin/CCK antisera was isolated from chicken antral extracts by a combination of gel filtration and reversed‐phase HPLC. The sequence was: Phe‐Leu‐Pro‐His‐Val‐Phe‐Ala‐Glu‐Leu‐Ser‐Asp‐Arg‐Lys‐Gly‐Phe‐Val‐Gln‐Gly‐Asn‐Gly‐Ala‐Val‐Glu‐Ala‐Leu‐His‐Asp‐His‐Phe‐Tyr‐Pro‐Asp‐Trp‐Met‐Asp‐Phe(NH2). Aside from the C‐terminal tetrapeptide and the Tyr residue, the molecule does not resemble other known forms of gastrin or CCK. The peptide was a potent stimulus of avian gastric acid but not pancreatic secretion. The results have important implications for the structure‐activity and evolutionary relationships of the gastrin/CCK family.
On the primary site of control in the spontaneous development of small‐intestinal sucrase‐isomaltase after birth
Authors: Sebastio Gianfranco , Hunziker Walter , Ballabio Andrea , Auricchio Salvatore , Semenza Giorgio
Published: July 2002

We have compared the appearance in the small intestine of baby rabbits, of sucrase and isomaltase activities and of the sucrase‐isomaltase mRNA. For the latter we used a cDNA probe encompassing ~4.1 kb from the 5'‐end of pro‐sucrase‐isomaltase cDNA [(1986) Cell 46, 227‐234]. Sucrase‐isomaltase mRNA and the enzyme activities developed simultaneously from the 15th day after birth onwards. Over two orders of magnitude the enzymatic activities and sucrase‐isomaltase mRNA matched one another closely, thus ruling out translation as the main site of biosynthetic control during spontaneous development, while rendering very probable transcription as the primary site of control. However, we cannot rule out the possibility that, prior to day 15, sucrase‐isomaltase mRNA might be degraded so rapidly that it is not translated.
Molecular cloning and sequencing of cDNA for rat cathepsin H Homology in pro‐peptide regions of cysteine proteinases
Authors: Ishidoh Kazumi , Imajoh Shinobu , Emori Yasufumi , Ohno Shigeo , Kawasaki Hiroshi , Minami Yasufumi , Kominami Eiki , Katunuma Nobuhiko , Suzuki Koichi
Published: July 2002

A cDNA for rat cathepsin H was isolated and sequenced. The deduced protein comprising 333 amino acid residues is composed of a typical signal sequence (21 residues), a pro‐peptide region (92 residues) and a mature enzyme region (220 residues). The amino acid sequence in the pro‐peptide region, in particular, residues Phe‐(−41) to Ser‐(−29) of cathepsin H, is highly homologous to the pro‐peptide regions of other cysteine proteinases. This homologous region may play a role in the processing of cysteine proteinases.
ATPase activity of the microvillar 110 kDa polypeptide‐calmodulin complex is activated in Mg2+ and inhibited in K+‐EDTA by F‐actin
Authors: Krizek Janet , Coluccio Lynne M. , Bretscher Anthony
Published: July 2002

Highly purified microvillar 110 kDa polypeptide‐calmodulin (110 K‐cam) complex was confirmed to have ATPase activities characteristic of a myosin. The effect of F‐actin on these activities was investigated. The Mg2+‐ATPase is activated about 2‐fold by F‐actin in a dose‐dependent fashion, whereas the K+‐EDTA ‐ATPase is inhibited by > 90% by F‐actin. These data provide evidence for a functional relationship between the ATPase activity of 110K‐cam and its interaction with F‐actin. They also extend the similarities between 110K‐cam and myosin. The results suggest that higher cells contain in addition to myosin a second class of myosin‐like molecules represented by 110K‐cam.
Intracellular distribution of DNA methyltransferase during the cell cycle
Authors: Vogel M.C. , Papadopoulos T. , Müller-Hermelink H.K. , Drahovsky D. , Pfeifer G.P.
Published: July 2002

The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nuclei with weak cytoplasmic staining. The DNA methyltransferase antigen was absent in early G1 phase, appeared in late G1 prior to the onset of DNA synthesis and persisted throughout S and G2 phases of the cell cycle. Mitotic cells showed a particularly strong staining intensity. These results show that DNA methyltransferase levels fluctuate during the cell cycle. This has possible implications on the stability of the DNA methylation pattern.
Isolation and analysis of the mouse opsin gene
Authors: Baehr W. , Falk J.D. , Bugra K. , Triantafyllos J.T. , McGinnis J.F.
Published: July 2002

We have identified three overlapping 5′‐truncated mouse opsin cDNA clones by immunologically screening a λgt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was interrupted by four introns positioned precisely as those previously described for other mammalian opsins. In contrast to the single major opsin mRNA in the bovine and human retina, Northern analysis of mouse retina RNA demonstrated the presence of at least five distinct species of polyadenylated opsin mRNAs. Their sizes ranged from 1.7 kb to 5.1 kb.
Inhibition of the amplified bombesin‐stimulated inositol phosphate response in N‐ras transformed cells by high density culturing
Authors: Wakelam Michael J.O.
Published: July 2002

The bombesin‐stimulated inositol phosphate response is only increased in cells transformed by the overexpression of N‐ras when they are cultured under sub‐confluent conditions. The inhibition of the amplified bombesin stimulation can be partially reversed by either, incubation of the cells for 5 h with 1 mM suramin, or by a 30 min preincubation of cells in excess buffer. The inhibitory effects are probably caused by the effects of autocrines and may explain some of the different published observations concerning the effects of ras gene products upon inositol phospholipid metabolism.
Activation of a brain‐specific protein kinase C subspecies in the presence of phosphatidylethanol
Authors: Asaoka Yoshinori , Kikkawa Ushio , Sekiguchi Kazuo , Shearman Mark S. , Kosaka Yoshiyuki , Nakano Yoshio , Satoh Tadashi , Nishizuka Yasutomi
Published: July 2002

Protein kinase C (PKC) is normally activated by diacylglycerol in the presence of Ca2+ and phosphatidylserine. At physiological concentrations of Ca2+, however, phosphatidylethanol, a product of the phospholipase D‐catalyzed transphosphatidylation reaction between membrane phospholipids and ethanol, can replace phosphatidylserine, and activate PKC. This mode of activation is most effective for the γ‐subspecies, which is expressed only in central nervous tissue. Phosphatidylmethanol is also effective to some extent. Consideration of these results suggests the possibility that ethanol may exert some effect on signal transduction in this tissue via changes in protein phosphorylation.