bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Feeding Frequency on Liver Transcriptome: Unveiling Appetite-Regulating Peptides in Mexican Pike Silverside (Chirostoma estor)
Authors: Juárez-Gutiérrez Mitzi Ernestina , Martínez-Chávez Carlos Cristian , Godoy-Figueroa Claudia Yaneth , Jiménez-Jacinto Verónica , Ríos-Durán María Gisela , Martínez-Palacios Carlos Antonio , Navarrete-Ramírez Pamela
Published: September 2024

The Mexican pike silverside (Chirostoma estor) is a zooplanktivorous, agastric short-intestined species, and it has been found that increased-frequency feeding (twelve feedings a day) improved feed efficiency and promoted growth by 70%. This work determined the effect of different juvenile feeding frequencies upon the C. estor liver transcriptome. The level of the expression of appetite-regulating peptides was analyzed in silico to understand the mechanisms involved in appetite control in this species. Differential expression analysis showed that up-regulated genes between treatments were related to metabolism, digestive processes, immune system response, apoptosis, growth, and oxidative stress. This information explains the better performance of pike silverside fed 12 times daily. Appetite regulatory peptides were identified for the first time in the liver of C. estor in response to high feeding frequencies, contributing to the general knowledge of the roles of each family of neuropeptides in this agastric, short-intestined fish. The information presented here emphasizes the need to explore further the complex physiological processes involved in appetite regulation in C. estor. Additionally, it will serve as a basis for more specific targeted studies of appetite control to elucidate the mechanisms behind this process.
Analyzing Hydrothermal Wave Transitions through Rotational Field Application Based on Entropy Production
Authors: Ban Takahiko , Fujiwara Ryo , Shigeta Keigo
Published: September 2024

In this study, we evaluated the nonlinear dynamics of convection flow using the thermodynamic variational principle, focusing on scenarios where multiple external forces, such as a thermal gradient and rotational field, are applied to a shallow annular pool. We observed that with the increase in the thermal gradient, the flow changed from an axial flow to a rotational oscillatory flow with the wave amplitudes aligned. Further increasing the temperature difference led to a rotational oscillatory flow characterized by alternating wave generation and annihilation. Our analysis of the flow, considering heat fluxes orthogonal to the thermal gradient, allowed us to describe the flow state as a phase at equilibrium. The state transition of the flow was accompanied by a discontinuous jump in the heat flux, which occurred at the intersection of the entropy production curves. The first transition occurred at a temperature difference ΔT=12.4 K Marangoni number,Ma=1716 and the second at ΔT = 16.3 K Ma=2255. Analysis based on entropy production could accurately predict the observed transition points.
Dynamics Analysis and Adaptive Synchronization of a Class of Fractional-Order Chaotic Financial Systems
Authors: Zhang Panhong , Wang Qingyi
Published: September 2024

It is of practical significance to realize a stable and controllable financial system by using chaotic synchronization theory. In this paper, the dynamics and synchronization are studied for a class of fractional-order chaotic financial systems. First, the stability and dynamics of the fractional-order chaotic financial system are analyzed by using the phase trajectory diagram, time series diagram, bifurcation diagram, and Lyapunov exponential diagram. Meanwhile, we obtain the range of each parameter that puts the system in a periodic state, and we also reveal the relationship of the derivative order and the chaotic behaviors. Then, the adaptive control strategy is designed to achieve synchronization of the chaotic financial system. Finally, the theoretical results and control method are verified by numerical simulations.
Distributed Adaptive Formation Control for Fractional-Order Multi-Agent Systems with Actuator Failures and Switching Topologies
Authors: Li Jing , Yan Zixiang , Shi Xingyun , Luo Xuqiong
Published: September 2024

In this paper, a class of distributed adaptive formation control problems are investigated for second-order nonlinear fractional-order multi-agent systems with actuator failures and switching topologies. To address these challenges, two adaptive coupling gains based on agents’ position and velocity are incorporated into the control protocol. Using the Lyapunov method along with graph theory and matrix analysis, sufficient conditions for system stability are derived in the presence of actuator failures and switching topologies. The effectiveness of the proposed control protocol is demonstrated through numerical simulations, which show its capability to maintain stable formation control under these challenging conditions.
Asymptotic Synchronization for Caputo Fractional-Order Time-Delayed Cellar Neural Networks with Multiple Fuzzy Operators and Partial Uncertainties via Mixed Impulsive Feedback Control
Authors: Fan Hongguang , Yi Chengbo , Shi Kaibo , Chen Xijie
Published: September 2024

To construct Caputo fractional-order time-delayed cellar neural networks (FOTDCNNs) that characterize real environments, this article introduces partial uncertainties, fuzzy operators, and nonlinear activation functions into the network models. Specifically, both the fuzzy AND operator and the fuzzy OR operator are contemplated in the master–slave systems. In response to the properties of the considered cellar neural networks (NNs), this article designs a new class of mixed control protocols that utilize both the error feedback information of systems and the sampling information of impulse moments to achieve network synchronization tasks. This approach overcomes the interference of time delays and uncertainties on network stability. By integrating the fractional-order comparison principle, fractional-order stability theory, and hybrid control schemes, readily verifiable asymptotic synchronization conditions for the studied fuzzy cellar NNs are established, and the range of system parameters is determined. Unlike previous results, the impulse gain spectrum considered in this study is no longer confined to a local interval (−2, 0) and can be extended to almost the entire real number domain. This spectrum extension relaxes the synchronization conditions, ensuring a broader applicability of the proposed control schemes.
State-Space Approach to the Time-Fractional Maxwell’s Equations under Caputo Fractional Derivative of an Electromagnetic Half-Space under Four Different Thermoelastic Theorems
Authors: Al-Lehaibi Eman A. N. , Youssef Hamdy M.
Published: September 2024

This paper introduces a new mathematical modelling method of a thermoelastic and electromagnetic half-space in the context of four different thermoelastic theorems: Green–Naghdi type-I, and type-III; Lord–Shulman; and Moore–Gibson–Thompson. The bunding plane of the half-space surface is subjected to ramp-type heat and traction-free. We consider that Maxwell’s time-fractional equations have been under Caputo’s fractional derivative definition, which is the novelty of this work. Laplace transform techniques are utilized to obtain solutions using the state-space approach. Laplace transform’s inversions were calculated using Tzou’s iteration method. The temperature increment, strain, displacement, stress, induced electric field, and induced magnetic field distributions were obtained numerically and are illustrated in figures. The time-fraction parameter of Maxwell’s equations had a major impact on all the studied functions. The time-fractional parameter of Maxwell’s equations worked as resistant to the changing of temperature, particle movement, and induced magnetic field, while it acted as a catalyst to the induced electric field through the material. Moreover, all the studied functions have different values in the context of the four studied theorems.
The Spatial Variation of Soil Structure Fractal Derived from Particle Size Distributions at the Basin Scale
Authors: He Yujiang , Peng Borui , Dai Lei , Wang Yanyan , Liu Ying , Wang Guiling
Published: September 2024

The accurate characterization of soil structure is fundamental to groundwater science, environmental ecology, and Earth systems science. To address the challenge of quantifying the high spatial variability of large-scale soil structures, this study used a laser particle size analyzer to measure the distribution of soil particle size in 207 samples from ten profiles across the Daqing and Ziya River basins in the North China Plain. The quantified soil structure, expressed as soil fractal dimension D, was derived using monofractal theory. Various spatial analysis techniques, including Moran’s I index, correlation analysis heat maps, the Kolmogorov–Smirnov one-sample test, and geostatistical semivariogram function, were jointly applied to investigate the spatial variability of soil structural fractals across different depths in the piedmont plain–coastal areas of the two river basins. The results indicate the following: (1) Quantitative analysis confirms that under the influence of piedmont alluvial and fluvial dynamics, soil D values homogenize from the piedmont to the coastal areas, with decreasing particle size differences closer to the coast. However, the spatial variability of the soil structural fractals in the Ziya River Basin was greater than that in the Daqing River Basin. (2) The combined effects of climate change, regional differences, and human activity led to greater spatial variability in the soil structural fractals in the Ziya River Basin than in the Daqing River Basin. The correlation between D values and burial depth was strongest in the Xianxian profile (−0.78), whereas the spatial correlation was strongest in the Hengshui and Dacheng profiles (−0.47). (3) The greatest spatial variability in soil D values occurred at depths of 1–2 m, with a coefficient of variation of 23.595%, which was significantly higher than those at depths of 0–1 (14.569%) and 2–3 m (16.284%).
Multifractal Analysis of the Impact of Fuel Cell Introduction in the Korean Electricity Market
Authors: Ock Seung Eun , Lee Minhyuk , Song Jae Wook
Published: September 2024

This study employs multifractal detrended fluctuation analysis to investigate the impact of fuel cell introduction in the Korean electricity market via the lens of multifractal scaling behavior. Using multifractal analysis, the research delineates discrepancies between peak and off-peak hours, accounting for the daily cyclicity of the electricity market, and proposes a crossover point detection method based on the Chow test. Furthermore, the impacts of fuel cell introduction are evidenced through various methods that encompass multifractal spectra and market efficiency. The findings initially indicate a higher degree of multifractality during off-peak hours relative to peak hours. In particular, the crossover points emerged solely during off-peak hours, unveiling short- and long-term dynamics predicated on a near-annual cycle. Additionally, the average Hurst exponent for the short-term was 0.542, while the average for the long-term was 0.098, representing a notable discrepancy. The introduction of fuel cells attenuated the heterogeneity in the scaling behavior, which is potentially attributable to the decreased volatility in both the supply and demand spectra. Remarkably, after the introduction of fuel cells, there was a discernible decrease in the influence of long-range correlation within multifractality, and the market exhibited an increased propensity toward random-walk behavior. This phenomenon was also detected in the market deficiency measure, from an average of 0.536, prior to the introduction, to an average of 0.267, following the introduction, signifying an improvement in market efficiency. This implies that the introduction of fuel cells into the market engendered increased supply stability and a consistent increase in demand, mitigating volatility on both the supply and demand sides, thus increasing market efficiency.
Analytic Approach Solution to Time-Fractional Phi-4 Equation with Two-Parameter Fractional Derivative
Authors: Massoun Youssouf , Alomari Abedel-Karrem , Cesarano Clemente
Published: September 2024

This paper is devoted to building a general framework for constructing a solution to fractional Phi-4 differential equations using a Caputo definition with two parameters. We briefly introduce some definitions and properties of fractional calculus in two parameters and the Phi-4 equation. By investigating the homotopy analysis method, we built the solution algorithm. The two parameters of the fractional derivative gain vary the behavior of the solution, which allows the researchers to fit their data with the proper parameter. To evaluate the effectiveness and accuracy of the proposed algorithm, we compare the results with those obtained using various numerical methods in a comprehensive comparative study.
On Extended Class of Totally Ordered Interval-Valued Convex Stochastic Processes and Applications
Authors: Javed Muhammad Zakria , Awan Muhammad Uzair , Ciurdariu Loredana , Dragomir Silvestru Sever , Almalki Yahya
Published: September 2024

The intent of the current study is to explore convex stochastic processes within a broader context. We introduce the concept of unified stochastic processes to analyze both convex and non-convex stochastic processes simultaneously. We employ weighted quasi-mean, non-negative mapping γ, and center-radius ordering relations to establish a class of extended cr-interval-valued convex stochastic processes. This class yields a combination of innovative convex and non-convex stochastic processes. We characterize our class by illustrating its relationships with other classes as well as certain key attributes and sufficient conditions for this class of processes. Additionally, leveraging Riemann–Liouville stochastic fractional operators and our proposed class, we prove parametric fractional variants of Jensen’s inequality, Hermite–Hadamard’s inequality, Fejer’s inequality, and product Hermite–Hadamard’s like inequality. We establish an interesting relation between means by means of Hermite–Hadamard’s inequality. We utilize the numerical and graphical approaches to showcase the significance and effectiveness of primary findings. Also, the proposed results are powerful tools to evaluate the bounds for stochastic Riemann–Liouville fractional operators in different scenarios for a larger space of processes.
Shear Mechanism and Optimal Estimation of the Fractal Dimension of Glass Bead-Simulated Sand
Authors: Li Xuefeng , Wang Rui
Published: September 2024

Spherical glass beads weaken the influences of particle morphology, surface properties, and microscopic fabric on shear strength, which is significant for revealing the relationship between macroscopic particle friction mechanisms and the particle size distribution of sand. This paper explores the shear mechanical properties of glass beads with different particle size ratios under different confining pressures. It obtains the particle size ratio and fractal dimension D through an optimal mechanical response. Simultaneously, we explore the range of the fractal dimension D under well-graded conditions. The test results show that the strain-softening degree of Rs is more obvious under a highly effective confining pressure, and the strain-softening degree of Rs can reach 0.669 when the average particle size d¯ is 0.5 mm. The changes in the normalized modulus ratio Eu/Eu50 indicate that the particle ratio and arrangement are the fundamental reasons for the different macroscopic shear behaviors of particles. The range of the peak effective internal friction angle φ is 23 °~35 °, and it first increases and then decreases with the increase in the effective confining pressure. As the average particle size increases, the peak stress ratio MFL and the peak effective internal friction angle φ first increase and then decrease, and both can be expressed using the Gaussian function. The range of the fractal dimension D for well-graded particles is 1.873 to 2.612, and the corresponding average particle size d¯ ranges from 0.433 to 0.598. Under the optimal mechanical properties of glass beads, the particle size ratio of 0.25 mm to 0.75 mm is 23:27, and the fractal dimension D is 2.368. The study results provide a reference for exploring friction mechanics mechanisms and the optimal particle size distributions of isotropic sand.
Matrix Compression and Pore Heterogeneity in the Coal-Measure Shale Reservoirs of the Qinshui Basin: A Multifractal Analysis
Authors: Zhong Baoyuan , Zhu Yanming , Feng Guangjun , Xiang Jie , Wang Yang
Published: September 2024

The application of high-pressure fluid induces the closure of isolated pores inside the matrix and promotes the generation of new fractures, resulting in a compressive effect on the matrix. To examine the compressibility of coal-measure shale samples, the compression of the coal–shale matrix in the high-pressure stage was analyzed by a low-pressure nitrogen gas adsorption and mercury intrusion porosimetry experiment. The quantitative parameters describing the heterogeneity of the pore-size distribution of coal-measure shale are obtained using multifractal theory. The results indicate that the samples exhibit compressibility values ranging from 0.154 × 10−5 MPa−1 to 4.74 × 10−5 MPa−1 across a pressure range of 12–413 MPa. The presence of pliable clay minerals enhances the matrix compressibility, whereas inflexible brittle minerals exhibit resistance to matrix compression. There is a reduction in local fluctuations of pore volume across different pore sizes, an improvement in the autocorrelation of PSD, and a mitigation of nonuniformity after correction. Singular and dimension spectra have advantages in multifractal characterization. The left and right spectral width parameters of the singular spectrum emphasize the local differences between the high- and low-value pore volume areas, respectively, whereas the dimensional spectrum width is more suitable for reflecting the overall heterogeneity of the PSD.
Concentrating Solutions for Fractional Schrödinger–Poisson Systems with Critical Growth
Authors: Shen Liejun , Squassina Marco
Published: September 2024

In this paper, we consider a class of fractional Schrödinger–Poisson systems (−Δ)su+λV(x)u+ϕu=f(u)+|u|2s*−2u and (−Δ)tϕ=u2 in R3, where s,t∈(0,1) with 2s+2t>3, λ>0 denotes a parameter, V:R3→R admits a potential well Ω≜intV−1(0) and 2s*≜63−2s is the fractional Sobolev critical exponent. Given some reasonable assumptions as to the potential V and the nonlinearity f, with the help of a constrained manifold argument, we conclude the existence of positive ground state solutions for some sufficiently large λ. Upon relaxing the restrictions on V and f, we utilize the minimax technique to show that the system has a positive mountain-pass type by introducing some analytic tricks. Moreover, we investigate the asymptotical behavior of the obtained solutions when λ→+∞.
Rheological Properties and Antioxidant Activity of Gelatin-Based Edible Coating Incorporating Tomato (Solanum lycopersicum L.) Extract
Authors: Estrella-Osuna Danya E. , Ruiz-Cruz Saul , Rodríguez-Félix Francisco , Figueroa-Enríquez Cielo E. , González-Ríos Humberto , Fernández-Quiroz Daniel , Márquez-Ríos Enrique , Tapia-Hernández José Agustín , Pérez-Álvarez José Ángel , Suárez-Jiménez Guadalupe Miroslava
Published: September 2024

Gelatin is a promising biopolymer for edible coatings thanks to its low cost and gelling properties. However, its weak mechanical properties limit its use. This study aimed to develop a gelatin coating with tomato extract, analyzing its antioxidant activity and rheological properties for food applications. Gelatin concentrations (2, 5, and 7%) were evaluated, and it was determined that 7% with 7.5% glycerol was the optimal mixture. Three concentrations of tomato extract (0.5, 1, and 1.5%) were added, and antioxidant activity was evaluated using the ABTS technique, as well as the interaction of components through FT-IR and physicochemical analysis. The results showed that there were no significant differences in terms of their physicochemical characterization, maintaining a pH of 5 and a yellowish hue. The FT-IR spectra indicated there were hydrogen bond interactions between gelatin and the extract. The antioxidant capacity was higher with the 1.5% extract, achieving an inhibition of 58.9%. It was found that the combination of the different materials used improved the rheological (specifically the viscosity and stability of the material) and antioxidant properties of the gelatin. These findings suggest that modified gelatin coatings may be effective in extending the shelf life of foods.
Antibiotic-Loaded Hydrogel for the Treatment of Lower-Limb Fracture-Related Infections: A Single Center’s Multidisciplinary Experience
Authors: De Meo Daniele , Martini Paolo , Lo Torto Federico , Petrucci Flavia , Ordonez Reyna Jessica , Candela Vittorio , Iaiani Giancarlo , Oliva Alessandra , Ribuffo Diego , Gumina Stefano
Published: September 2024

A fracture-related infection (FRI) is a severe complication of an orthopedic trauma, often leading to challenging treatments and poor outcomes. The surgical strategies are typically categorized into one-stage or two-stage procedures, with the use of systemic and local antibiotics being crucial for infection management. This study assessed the efficacy of an antibiotic-loaded hydrogel (ALH) applied over the internal fixation devices for treating FRIs, comparing the outcomes between the one-stage (OS) and two-stage (TS) reconstructions. This retrospective study included 17 patients with an FRI treated using the ALH at a single center. The patients were divided into OS and TS reconstruction groups. The data on demographics, surgical procedures, antibiotic regimens, and outcomes were collected. The primary and secondary outcomes included the infection cure rate, bone union, complications, and reoperation rates. Among the 17 patients (mean age 48.5 years, 16 males), infections were predominantly in the tibia, with 12 chronic and 5 acute cases. Seven patients had monomicrobial infections, and nine had multidrug-resistant pathogens. No significant differences were found between the OS and TS groups in terms of the infection cure rate, bone union, or complications. One patient in the OS group experienced an infection recurrence, and bone healing was achieved in all but one case. Additional complications included delayed wound closure in two cases and implant failure in one case, requiring a reoperation. The ALH demonstrated potential as an effective local antibiotic treatment for FRIs, particularly in the one-stage reconstructions, allowing for a safe application of internal fixation devices. However, further research with larger sample sizes and longer follow-ups is needed to validate these findings.
New Supramolecular Hydrogels Based on Diastereomeric Dehydrotripeptide Mixtures for Potential Drug Delivery Applications
Authors: Oliveira Carlos B. P. , Carvalho André , Pereira Renato B. , Pereira David M. , Hilliou Loic , Jervis Peter J. , Martins José A. , Ferreira Paula M. T.
Published: September 2024

Self-assembly of peptide building blocks offers unique opportunities for bottom-up preparation of exquisite nanostructures, nanoarchitectures, and nanostructured bulk materials, namely hydrogels. In this work we describe the synthesis, characterization, gelation, and rheological properties of new dehydrotripeptides, Cbz-L-Lys(Cbz)-L,D-Asp-∆Phe-OH and (2-Naph)-L-Lys(2-Naph)-L,D-Asp-∆Phe-OH, containing a N-terminal lysine residue Nα,ε-bis-capped with carboxybenzyl (Cbz) and 2-Naphthylacetyl (2-Naph) aromatic moieties, an aspartic acid residue (Asp), and a C-terminal dehydrophenylalanine (∆Phe) residue. The dehydrotripeptides were obtained as diastereomeric mixtures (L,L,Z and L,D,Z), presumably via aspartimide chemistry. The dehydrotripeptides afforded hydrogels at exceedingly low concentrations (0.1 and 0.04 wt%). The hydrogels revealed exceptional elasticity (G’ = 5.44 × 104 and 3.43 × 106 Pa) and self-healing properties. STEM studies showed that the diastereomers of the Cbz-capped peptide undergo co-assembly, generating a fibrillar 3D network, while the diastereomers of the 2-Naph-capped dehydropeptide seem to undergo self-sorting, originating a fibril network with embedded spheroidal nanostructures. The 2-Naph-capped hydrogel displayed full fast recovery following breakup by a mechanical stimulus. Spheroidal nanostructures are absent in the recovered hydrogel, as seen by STEM, suggesting that the mechanical stimulus triggers rearrangement of the spheroidal nanostructures into fibers. Overall, this study demonstrates that diastereomeric mixtures of peptides can be efficacious gelators. Importantly, these results suggest that the structure (size, aromaticity) of the capping group can have a directing effect on the self-assembly (co-assembly vs. self-sorting) of diastereomers. The cytotoxicity of the newly synthesized gelators was evaluated using human keratinocytes (HaCaT cell line). The results indicated that the two gelators exhibited some cytotoxicity, having a small impact on cell viability. In sustained release experiments, the influence of the charge on model drug compounds was assessed in relation to their release rate from the hydrogel matrix. The hydrogels demonstrated sustained release for methyl orange (anionic), while methylene blue (cationic) was retained within the network.
BioMOF@cellulose Glycerogel Scaffold with Multifold Bioactivity: Perspective in Bone Tissue Repair
Authors: Rosado Albert , Borrás Alejandro , Sánchez-Soto Miguel , Labíková Magdaléna , Hettegger Hubert , Ramírez-Jiménez Rosa Ana , Rojo Luís , García-Fernández Luís , Aguilar María Rosa , Liebner Falk , López-Periago Ana M. , Ayllón José A. , Domingo Concepción
Published: September 2024

The development of new biomaterials for musculoskeletal tissue repair is currently an important branch in biomedicine research. The approach presented here is centered around the development of a prototypic synthetic glycerogel scaffold for bone regeneration, which simultaneously features therapeutic activity. The main novelty of this work lies in the combination of an open meso and macroporous nanocrystalline cellulose (NCC)-based glycerogel with a fully biocompatible microporous bioMOF system (CaSyr-1) composed of calcium ions and syringic acid. The bioMOF framework is further impregnated with a third bioactive component, i.e., ibuprofen (ibu), to generate a multifold bioactive system. The integrated CaSyr-1(ibu) serves as a reservoir for bioactive compounds delivery, while the NCC scaffold is the proposed matrix for cell ingrowth, proliferation and differentiation. The measured drug delivery profiles, studied in a phosphate-buffered saline solution at 310 K, indicate that the bioactive components are released concurrently with bioMOF dissolution after ca. 30 min following a pseudo-first-order kinetic model. Furthermore, according to the semi-empirical Korsmeyer-Peppas kinetic model, this release is governed by a case-II mechanism, suggesting that the molecular transport is influenced by the relaxation of the NCC matrix. Preliminary in vitro results denote that the initial high concentration of glycerol in the NCC scaffold can be toxic in direct contact with human osteoblasts (HObs). However, when the excess of glycerol is diluted in the system (after the second day of the experiment), the direct and indirect assays confirm full biocompatibility and suitability for HOb proliferation.
The Impact of Anchoring on Seafloor Integrity: An Integrated Assessment within a Major Bunkering Area of the Maltese Islands
Authors: Mizzi Michelle , Deidun Alan , Gauci Adam , Gauci Ritienne
Published: September 2024

International shipping is a fundamental component of the global economy. As the industry expands, the demand for high-capacity vessels increases, raising concerns about their impact on the marine environment. While awaiting access to port facilities, vessels often anchor to save fuel and prevent drifting, but this practice is a significant cause of mechanical disturbance to the seafloor and benthic habitats. Identifying and quantifying anchoring pressure is essential for better managing and mitigating the damage to the seafloor. The Automatic Identification System (AIS) can be utilized to assess anchoring pressure by transmitting vessel information (e.g., position, type and size) to other vessels and coastal stations. This research evaluates anchoring pressure in a strategically located bunkering area around the Maltese Islands using AIS data collected from an antenna at the University of Malta. An arbitrary index was developed to determine anchoring pressure, and the AIS data was used to create GIS maps showing the location and size of vessels within the bunkering area, as well as plots depicting anchoring pressure by vessel type, seasonality, and density. This study serves as a blueprint for future assessments of anchoring pressures from various maritime activities in other areas around the Maltese Islands and provides a decision support tool for national policy-making related to Descriptor 6 (Seafloor Integrity) of the Marine Strategy Framework Directive (MSFD), the Maritime Spatial Planning Directive (MSPD), and the management plan for Sites of Community Interest (SCI) and Marine Protected Areas (MPAs).
Tactile Simultaneous Localization and Mapping Using Low-Cost, Wearable LiDAR
Authors: LaRocco John , Tahmina Qudsia , Simonis John , Liang Taylor , Zhang Yiyao
Published: September 2024

Tactile maps are widely recognized as useful tools for mobility training and the rehabilitation of visually impaired individuals. However, current tactile maps lack real-time versatility and are limited because of high manufacturing and design costs. In this study, we introduce a device (i.e., ClaySight) that enhances the creation of automatic tactile map generation, as well as a model for wearable devices that use low-cost laser imaging, detection, and ranging (LiDAR,) used to improve the immediate spatial knowledge of visually impaired individuals. Our system uses LiDAR sensors to (1) produce affordable, low-latency tactile maps, (2) function as a day-to-day wayfinding aid, and (3) provide interactivity using a wearable device. The system comprises a dynamic mapping and scanning algorithm and an interactive handheld 3D-printed device that houses the hardware. Our algorithm accommodates user specifications to dynamically interact with objects in the surrounding area and create map models that can be represented with haptic feedback or alternative tactile systems. Using economical components and open-source software, the ClaySight system has significant potential to enhance independence and quality of life for the visually impaired.
Diagnostic Performance of Host and Viral DNA Methylation Analysis in the Identification of Anal Intraepithelial Neoplasia and Cancer: Systematic Review and Meta-Analysis
Authors: Muresu Narcisa , Puci Mariangela Valentina , Sotgiu Giovanni , Sechi Illari , Cossu Andrea , Usai Manuela , Piana Andrea Fausto
Published: September 2024

Introduction: DNA methylation-based biomarkers have been investigated as useful tools in the carcinogenesis process, including the triage of HPV-associated cancers. In this context, we conducted a systematic review and meta-analysis focused on evaluating the changes in the level of DNA methylation in cases of pre-cancerous (i.e., anal intraepithelial neoplasia, AIN-1, -2., -3) and cancerous (i.e., squamous cell carcinoma, SCC) anal lesions. Methods: A research in the PubMed, Scopus, and Web of Science databases was carried out, following the PRISMA 2020 protocol, using the following keywords: “anal cancer”, “anal intraepithelial neoplasia”, “methylation”, and “epigenetic”. All observational studies that reported the level of DNA methylation by grade of anal lesions and for different target genes were included. The QUADAS-2 tool was used to assess the studies’ quality, whereas pooled prevalence, sensitivity, specificity, and diagnostic odds ratio (DOR) were employed to verify the accuracy of the test in the detection of high-grade lesions. Results: Eight studies met the inclusion criteria, involving a total of 1555 clinical samples. The prevalence of methylation-positive samples by histological grading was 27%, 45%, 54%, and 98% for AIN1, AIN2, AIN3, and SCC, respectively. Similar results were observed for the DOR, with higher ORs in more severe lesions. The pooled AUC (95%CI) for the diagnosis of ≥AIN2 was 0.68 (0.63–0.73). Conclusions: The present review and meta-analysis support the introduction of DNA methylation-based biomarkers in the triage of subjects with low-grade anal lesions and in the monitoring of treatment outcomes. Standardized protocols and a prospective study design are needed to implement methylation tests in clinical practice.
Perceptions and Interest in Lung Cancer Screening by Smoking Status: A Cross-Sectional Study of HINTS 6 (2022)
Authors: Lin Wenxue , Alasqah Ibrahim , Alotaibi Saad A. , Alqarawi Nada , Almutairi Sulaiman Sulmi , Saraiva Ariana , Raposo António
Published: September 2024

Background: Lung cancer screening guidelines prioritize individuals with a history of smoking due to their higher risk of the disease. Methods: Our study examines the awareness and interest in low-dose computed tomography (LDCT) lung cancer screening among different smoking statuses using data from the National Cancer Institute’s Health Information National Trends Survey (HINTS) 6 (2022). We analyzed data from HINTS 6, including 3915 participants on smoking status, LDCT screening, and telehealth use. Participants were categorized as current smokers, former smokers, and non-smokers. Results: Current smokers had the highest likelihood of being recommended for LDCT screening (OR: 7.1, aOR: 10.4) compared with non-smokers. Former smokers also had increased odds of screening recommendations (OR: 3.1, aOR: 3.4) than non-smokers. Despite higher screening recommendations, current smokers exhibited significantly lower interest in cancer screening (interest rating score: 2.1) compared with non-smokers (interest rating score: 2.4) and former smokers (interest rating score: 2.5). Current smokers rated their telehealth care experiences more positively in terms of care quality compared with non-smokers. Conclusions: Our findings underscore a gap in cancer screening interest among current smokers despite their higher likelihood of being recommended for LDCT screening. The favorable perception of telehealth among current smokers provides an opportunity to enhance engagement and promote LDCT scan through telehealth care.
Translation, Cultural Adaptation of the Portuguese Provider Attitudes toward Cardiac Rehabilitation and Referral (PACRR-P) Scale and Assessment of Its’ Measurement Properties
Authors: Cruz Mayara Moura Alves da , Vanderlei Luiz Carlos Marques , Takahashi Carolina , Laurino Maria Julia Lopez , Cruz Murilo Reis Alves da , Grace Sherry L. , Ghisi Gabriela L. M.
Published: September 2024

Background/Objectives: Access to cardiac rehabilitation (CR) is contingent upon physician referrals, yet these are often inadequate, particularly in low-resource settings. This multi-method study aimed to translate, culturally adapt, and validate the Portuguese version of the Provider Attitudes toward CR and Referral (PACRR-P) scale, as well as to identify key factors influencing CR referral in a Latin American context for the first time. Methods: The PACRR was translated into Brazilian Portuguese through a rigorous process involving independent translation, back-translation, and expert panel review to ensure face, content, and cross-cultural validity. A total of 44 Brazilian physicians completed the questionnaire, allowing for an assessment of internal consistency, criterion validity, and convergent validity. Results: The findings confirmed the face, content, and cultural validity of the 20 translated items, with a mean item clarity rating of 4.8/5. The final version included 17 of the original 19 PACRR-P items, with a Cronbach’s alpha of 0.73. Referral rates were significantly associated with over one-third of the PACRR-P items, preliminarily supporting the scale’s criterion validity, while correlations with the ReCaRe scores further supported its convergent validity. The most prominent barriers to referral were a lack of familiarity with CR site locations, absence of a standard referral form, and lack of automatic referral processes. Conclusions: The PACRR’s validity and reliability among Portuguese-speaking providers are preliminarily supported.
Qarasiña Culinary Tradition: Conserving Quinoa (Chenopodium quinoa) as an Intangible Cultural Heritage in Jach’a Puni (Andean Community), Bolivia
Authors: Cocarico Simón , Rivera Diego , Beck Stephan , Obón Concepción
Published: September 2024

Heritage encompasses features integral to the culture of a particular society, such as traditional foods that have historical significance and continue to be part of the community’s identity. These culinary traditions must be preserved to sustain cultural identity and community well-being. This study aims to document the role of qarasiña, along with other ancestral foods, in establishing quinoa (Chenopodium quinoa) as a cultural keystone species in the Jach’a Puni community. Primary data were gathered through in-depth interviews with local experts and community observations, supplemented by a comprehensive literature review. Findings reveal that in Jach’a Puni, all food is considered sacred. Three main types of qarasiña are identified: jupha qarasiña, ajara qarasiña, and kita qarasiña. The primary ingredients are domesticated quinoa (jupha) flour (Chenopodium quinoa var. quinoa) and wild quinoa (ajara) flour (Chenopodium quinoa var. melanospermum and C. hircinum). Qarasiña is integral to community banquets and social events. Although quinoa is celebrated globally as the “golden grain”, there is a risk that qarasiña may disappear and quinoa could be replaced by wheat or maize, threatening its status as a cornerstone species in the Bolivian Altiplano.
The Three-Dimensional Model as a ‘Scientific Fact’: The Scientific Methodology in Hypothetical Reconstruction
Authors: Apollonio Fabrizio I.
Published: September 2024

For more than thirty years, 3D digital modelling has been used more and more widely as a research tool in various disciplinary fields. Despite this, the 3D models produced by different research, investigation, and speculation activities are still only used as a basis and as sources for the production of images and scientific contributions (papers in journals, contributions in conference proceedings, etc.) in dissemination and cultural activities, but without having yet assumed full autonomy as a ‘scientific fact’, as a product of research, or as a means of scientific debate and progress. This paper outlines the context in the field of architecture and archeology in which the use of 3D models has become increasingly widespread, reaching a level of full maturity, and how the field of hypothetical reconstruction can be characterized as an autonomous/scientific discipline through the definition and adoption of a scientific, transparent, verifiable, reusable, and refutable method. In this context, the definition of the 3D model as a product of scientific speculation and research is proposed.
Mosaic Technology in the Armenian Chapel Birds Mosaic, Jerusalem: Characterizing the Polychrome Hidden Sinopia
Authors: Asscher Yotam , Ricci Giulia , Reato Michela , Peters Ilana , Leviant Abraham , Neguer Jacques , Avrahami Mark , Artioli Gilberto
Published: September 2024

Since the Hellenistic period, preparatory drawings known as sinopiae were employed as guidelines for mosaicists in creating mosaics. The sinopiae served as the basis for style and content, facilitating the placement of colored tesserae in the supporting mortar. The technology of the mosaic and pigments used reflect the capacity of the mosaic workshop and its master. This work explores a polychrome sinopia that was found under a Byzantine mosaic of an Armenian Chapel in Jerusalem, by a multi-analytical characterization of mineralogical and chemical properties. The composition of the pigments in the black and red areas of the sinopia include carbon black and red ochre, respectively, utilized in the fresco technique. Since colored tesserae are placed in wet mortar, it can be deduced that mosaicists worked together with painters during the executionary steps. This has corresponding implications for historical and artistic specializations at mosaic workshops, with deeper understanding of mosaic production processes. This research also highlights the importance of studying sinopiae under floor mosaics, which is a source of information on the pigments, paintings techniques, and the people who executed the work, all embedded in mortar which is well protected below the stone tesserae.
Classification of Vase Life Day Based on Petal Colorimetric Data in Cut Lisianthus Using AutoML
Authors: Kwon Hye Sook , Heo Seong
Published: September 2024

This study investigated the potential of petal colorimetric data to classify vase life stages in cut lisianthus flowers (Eustoma grandiflorum). We analyzed the changes in the petal color space over time, focusing on the b* value as an indicator of senescence due to increasing yellowing caused by copigmentation. A comparative analysis was conducted between two cultivation methods: soil (S) and hydroponic (H) cultivation. The objective was to evaluate the performance of machine learning models trained to classify vase life stages based on petal color data. Automated machine learning models exhibited better performance in H-cultivated cut flowers, effectively distinguishing days within the vase life stages from Days 1 to 14 for H cultivation. Cut flowers cultivated under S conditions showed less variation in the color space from Days 1 to 9, maintaining a relatively uniform color range. This made it more difficult to distinguish the vase life stages compared to H cultivation. These findings demonstrate that petal color metrics can serve as reliable indicators of cut flower senescence and potentially facilitate nondestructive methods for classifying vase life stages. This technology holds promise for wider applications in the floriculture industry, improving quality control, and extending the vase life of various cut-flower crops.
Uncovering Tourist Visit Intentions on Social Media through Sentence Transformers
Authors: Fantozzi Paolo , Maccario Guglielmo , Naldi Maurizio
Published: September 2024

The problem of understanding and predicting tourist behavior in choosing their destinations is a long-standing one. The first step in the process is to understand users’ intention to visit a country, which may later translate into an actual visit. Would-be tourists may express their intention to visit a destination on social media. Being able to predict their intention may be useful for targeted promotion campaigns. In this paper, we propose an algorithm to predict visit (or revisit) intentions based on the texts in posts on social media. The algorithm relies on a neural network sentence-transformer architecture using optimized embedding and a logistic classifier. Employing two real labeled datasets from Twitter (now X) for training, the algorithm achieved 90% accuracy and balanced performances over the two classes (visit intention vs. no-visit intention). The algorithm was capable of predicting intentions to visit with high accuracy, even when fed with very imbalanced datasets, where the posts showing the intention to visit were an extremely small minority.
Ultrastructure of Immatures Stages and Life Cycle of Helicobia aurescens (Diptera: Sarcophagidae: Sarcophaginae)
Authors: Cortinhas Lucas Barbosa , Mendonça Paloma Martins , Perrut Eliane Gomes , Barbosa Rodrigo Rocha , Santos-Mallet Jacenir Reis dos , Queiroz Margareth Maria de Carvalho
Published: September 2024

Helicobia aurescens is a flesh fly associated with pig and rat carcasses. This study aims to describe the life cycle at two temperatures (27 ± 1 °C and 29 ± 1 °C) and analyze the morphological characteristics of the eggs, larvae, and puparia of H. aurescens using scanning electron microscopy (SEM). Temperature is an abiotic factor that greatly influences the development of insects. The larval development of H. aurescens lasts longer at 27 ± 1 °C than it does at 29 ± 1 °C, affecting the growth of newly hatched larvae into adults. The females larviposited three times more at 27 °C than they did at 29 °C, and the number of days laying larvae was also greater. At 27 °C, they laid larvae for 38 days, whereas, at 29 °C, the females larviposited for 21 days. No hatching lines or median areas were detected on the exochorion with SEM, as in the other sarcophagid species. The first-instar larva is very similar to those of the other species. The second instar has anterior spiracles present on the first thoracic segment, with six or seven ramifications aligned regularly. On the third instar, these structures have eight ramifications in a regular row and are located dorsolaterally. The puparium morphology is similar to that of the third-instar larvae.
Non-Feeding Transmission Modes of the Tomato Yellow Leaf Curl Virus by the Whitefly Bemisia tabaci Do Not Contribute to Reoccurring Leaf Curl Outbreaks in Tomato
Authors: Marchant Wendy G. , Brown Judith K. , Gautam Saurabh , Ghosh Saptarshi , Simmons Alvin M. , Srinivasan Rajagopalbabu
Published: September 2024

Tomato yellow leaf curl virus (TYLCV) causes significant yield loss in tomato production in the southeastern United States and elsewhere. TYLCV is transmitted by the whitefly Bemisia tabaci cryptic species in a persistent, circulative, and non-propagative manner. Unexpectedly, transovarial and sexual transmission of TYLCV has been reported for one strain from Israel. In this study, the potential contribution of the B. tabaci B cryptic species transovarial and sexual transmission of TYLCV (Israel strain, Georgia variant, Georgia, USA) to reoccurring outbreaks was investigated by conducting whitefly-TYLCV transmission assays and virus DNA detection using end point PCR, DNA quantitation via real-time PCR, and virion detection by immunocapture PCR. TYLCV DNA was detectable in four, two, and two percent of first-generation fourth-instar nymphs, first-generation adults, and second-generation adults, respectively, following transovarial acquisition. Post-mating between viruliferous counterparts, the virus’s DNA was detected in four percent of males and undetectable in females. The accumulation of TYLCV DNA in whiteflies from the transovarial and/or sexual experiments was substantially lower (100 to 1000-fold) compared with whitefly adults allowed a 48-hr acquisition-access period on plants infected with TYLCV. Despite the detection of TYLCV DNA in whiteflies from the transovarial and/or mating experiments, the virions were undetectable by immunocapture PCR—a technique specifically designed to detect virions. Furthermore, tomato test plants exposed to whitefly adults that presumably acquired TYLCV transovarially or through mating remained free of detectable TYLCV DNA. Collectively, the extremely low levels of TYLCV DNA and complete absence of virions detected in whiteflies and the inability of the B. tabaci cryptic species B to transmit TYLCV to test tomato plants following transovarial and mating acquisition indicate that neither transovarial nor sexual transmission of TYLCV are probable or epidemiologically relevant for TYLCV persistence in this pathosystem.