bluuebunny/crossref_metadata_2025 huggingface dataset:
A Safety Evaluation Method for Converter Station Operation Based on Dynamic Fuzzy Theory
Authors: Song Yanzhe , Zhuang Yanjie , Fei Wenxin , Wan Jiashan , Xia Na
Published: November 2024

In order to accurately evaluate the safety risk level of converter station operations and reflect its changing trends, an evaluation method based on dynamic fuzzy theory is proposed. By constructing an initial Work Breakdown Structure-Risk breakdown structure (WBS-RBS) coupled matrix, identifying risk factors, and using the vague set screening method to screen out key risk factors, a comprehensive safety evaluation index system for converter station operations is established. The dynamic fuzzy values of all levels of evaluation indicators are calculated using dynamic fuzzy theory, intuitively reflecting the risk level and its changing trends of all levels of evaluation indicators. Taking the Suzhou ±800 kV converter station project as an example to verify this method, the results show that the safety risk level of this converter station operation is medium–low and tends to be low. This method can objectively reflect the ambiguity and randomness of risk evaluation, improve evaluation accuracy, and intuitively display the risk-changing trends, providing a reference for similar projects.
Performance and Optimization of Novel Solar-Assisted Heat Pump System with Hybrid Thermal Energy Storage
Authors: Ren Chaojie , Lin Jie , Guo Nini
Published: November 2024

In this study, a novel solar-assisted heat pump (SAHP) system with hybrid thermal energy storage is proposed. The system can address the problems of large space requirements and the unstable heating of solar heating systems and tackle the energy-efficient degradation of air source heat pumps (ASHPs) in winter. This study utilized TRNSYS18 software to establish a dynamic simulation model of the system, including the system’s model construction and the control scheme’s design. This performance study focused on analyzing the effects of the collector area and thermal energy storage (TES). The results show that with the increase in the collector area, the collector and power generation efficiencies decrease, and the system performance coefficient improves; the rise in the volume of TES leads to the collector and power generation efficiencies first increasing, and then they tend to stabilize, and the performance coefficient shows a trend of firstly increasing, and then decreasing. In terms of parameter optimization, a target optimization scheme and an evaluation model are constructed. The results indicate that the heating demand for a 116-square-meter building in the Tianjin area is met. The equivalent annual cost (EAC) of the system cost is the lowest, which is CNY 3963, when the collector area of the system is 31 square meters, the heat storage tank (HST) volume is 0.4 cubic meters and the phase-change energy storage (PCES) volume is 0.2 cubic meters. The payback period of the system is 10.59 years, which was compared to that of the ASHP. The further comparison of the economic feasibility of the system in the Lhasa, Shenyang, and Tianjin regions shows that the Lhasa region has the lowest EAC and payback period of CNY 1579 and 8.53 years, respectively, while the payback periods of Tianjin and Shenyang are 10.59 and 10.3 years, with EACs of CNY 3963 and CNY 5096, respectively.
Analysis of the Impact of Photovoltaic Generation on the Level of Energy Losses in a Low-Voltage Network
Authors: Gawlak Anna , Kornatka Mirosław
Published: November 2024

Due to the dynamic development of energy generation in photovoltaic installations, a reliable assessment of their impact on the level of energy losses in distribution networks is needed. For energy companies managing network resources, this issue has a very tangible practical aspect. Therefore, ongoing analyses of the level of electricity losses based on actual measurement data of prosumers are needed. In the paper, the influence of energy introduced by prosumer photovoltaic installations on energy losses in a low-voltage radial line is investigated. The issue is examined from three perspectives: 1. Focused on energy supplied into the low-voltage grid from photovoltaic installations; 2. the installations’ locations; and 3. the product of energy and distance from the power source. Comparative assessments are made of the examined aspects for 87 possible locations of prosumer installations in the tested low-voltage network. An analysis of energy losses is carried out both for the entire analysed network and separately for the line and the transformer. The changes in energy losses are influenced by both the power and the location of the photovoltaic installations. Based on the research findings, functions defining relative changes in energy losses in the low-voltage network are determined.
Performance Evaluation of Battery Swapping Stations for EVs: A Multi-Method Simulation Approach
Authors: Marchesano Maria Grazia , Popolo Valentina , Rozhok Anastasiia , Cavalaglio Gianluca
Published: November 2024

This study presents an optimisation framework for operating a battery swapping station (BSS) to enhance efficiency and sustainability in electric vehicle (EV) infrastructure. A hybrid modelling approach combines agent-based discrete event simulation and linear programming to model the dynamic behaviour of batteries and operational processes within the BSS. The model considers factors such as charging speed, battery degradation, grid power constraints, customer behaviour, and range anxiety. The agent-based model simulates the interaction between vehicles, batteries, and the station, capturing the stochastic nature of EVs’ arrivals and battery demand with the discrete event simulation. The linear programming component optimises battery state transitions to minimise degradation and ensure that the demand is met while respecting the power limits of the grid. Different battery types are considered based on vehicle category, each with specific capacity and usage patterns, reflecting real-world market conditions. The results demonstrate that the proposed optimisation framework can effectively manage the complex operational needs of a BSS. The proposed framework effectively balances service quality with resource efficiency by employing a strategic mix of charging modes and inventory management, reducing operational and degradation costs. This approach supports a more sustainable EV infrastructure, highlighting BSS as a viable solution to enhance the efficiency and sustainability of EV operations. Furthermore, the analysis highlights the critical role of power limits in determining charging strategies and their impact on operational efficiency. The findings suggest that with optimised operations, BSS can play a critical role in accelerating the adoption of EVs by offering a faster, more reliable, and sustainable alternative.
An Evaluation Model for the Firefighting and Rescue Capability of Cultural Relic Building Complexes in Forest Areas
Authors: Yan Long , Zheng Jiaxin , Li Qi , Zhang Guodong
Published: November 2024

Previous evaluation models for cultural relic buildings in relation to fire risk fail to consider the necessity for effective firefighting and rescue capabilities in complex forest environments. This paper incorporates variables, including those pertaining to forest fires and climatic conditions, into the assessment index system. The hierarchical analysis method and the local punishment-incentive variable weighting method are employed to introduce a compensation coefficient score. A model for the evaluation of firefighting and rescue capability in a continuous area of cultural relic buildings in conjunction with the surrounding forest environment has been developed. The firefighting and rescue capability of the Yuelu Mountain scenic spot was evaluated at 73.91 (level III) using the fixed weight method and 69.52 (level IV) using the variable weight method. The variable weight method proved to be a more accurate approach for evaluating the status and importance of dynamic targets, thus enabling a more precise evaluation of the comprehensive evaluation area. The evaluation results inform the formulation of targeted improvement measures for enhancing the firefighting and rescue capabilities of cultural relic buildings.
Shelf Life Study of Chilled Mullet (Mugil cephalus): Histamine Formation and Quality Degradation at Constant and Dynamic Storage Conditions
Authors: Ntzimani Athina , Papamichail Eirini , Dermesonlouoglou Efimia , Tsironi Theofania , Taoukis Petros
Published: November 2024

The present work aimed to evaluate and mathematically model the effect of temperature on Morganella morganii growth and histamine formation in farmed mullet (Mugil cephalus) during refrigerated storage (at constant temperatures, T = 0, 2.5, 5, 10, and 15 °C) and to validate the developed models at non-constant temperature conditions (effective temperature Teff = 7.4 °C). Shelf life evaluation of chilled mullet was also carried out based on microbial spoilage, sensory degradation, and total volatile nitrogen (TVB-N) determination. Spoilage of mullet during refrigerated storage was co-dominated by Pseudomonas spp. and Enterobacteriaceae growth. Sensory rejection (score 5 for overall impression) and the end of shelf life coincided with a total microbial load of 8 log cfu/g. The shelf life of chilled mullet was estimated at 15, 11, 7, 3, and 1.5 days at 0, 2.5, 5, 10, and 15 °C, respectively. At T 0–5 °C, the time of sensory rejection coincided with TVB-N concentrations of 10.2–12.3 mg·100 g−1, and at 10–15 °C, the samples were sensorially rejected before TVB-N development. At storage temperatures < 5 °C, sensory rejection was observed well before histamine levels reached a concentration of 50 mg/kg fish flesh. However, when abusive temperatures prevail, histamine should be considered as a risk factor for the human consumption of mullet.
Aqueous Extract of Lycium ruthenicum Murray Attenuates Neuroinflammation in C57BL/6J Mice Induced by High-Fat and High-Fructose Diet Through Regulating Gut Microbiota and Bile Acid Metabolism
Authors: Fan Xia , Dong Wei , Huang Yujie , Shu Yifan , Yan Yamei , Mi Jia , Lu Lu , Zeng Xiaoxiong , Cao Youlong
Published: November 2024

The aqueous extract of Lycium ruthenicum Murray (LRE) could attenuate neuroinflammation in mice induced by a high-fat and high-fructose diet (HFFD). Moreover, LRE could adjust bile acid (BA) metabolism and the gut microbiota. Behavioral tests revealed that LRE prevented HFFD-induced cognitive deficits. The treatment of LRE resulted in a decreased expression of inflammation-related mRNA of TNF-α, IL-6, and IL-1β in the cerebral cortex and hippocampus. Furthermore, LRE ameliorated gut microbiota disorder caused by HFFD by markedly elevating the relative abundances of Streptococcus and probiotics such as Lactococcus. Concurrently, it reduced the relative abundances of Helicobacter and Clostridium_XIVa. The levels of tauroursodeoxycholic acid, known for its neuroprotective property, and taurocholic acid, recognized as an anti-inflammatory agent, were significantly enhanced in the hippocampus and cerebral cortex due to the treatment with LRE. In a word, LRE might have the potential to alleviate HFFD-induced cognitive dysfunction by modulating intestinal microbiota and promoting the synthesis of neuroprotective BAs.
Occurrence, Antibiotic Resistance and Biofilm-Forming Ability of Listeria monocytogenes in Chicken Carcasses and Cuts
Authors: Panera-Martínez Sarah , Capita Rosa , Pedriza-González Ángela , Díez-Moura María , Riesco-Peláez Félix , Alonso-Calleja Carlos
Published: November 2024

A total of 104 samples of chicken meat acquired on the day of slaughter from two slaughterhouses in northwestern Spain were analyzed. These comprised 26 carcasses and 26 cuts from each of the two establishments. An average load of 5.39 ± 0.61 log10 cfu/g (total aerobic counts) and 4.90 ± 0.40 log10 cfu/g (psychrotrophic microorganisms) were obtained, with differences (p < 0.05) between types of samples and between slaughterhouses. Culturing methods involving isolation based on the UNE-EN-ISO 11290-1:2018 norm and identification of isolates by polymerase chain reaction (PCR) to detect the lmo1030 gene allowed the detection of Listeria monocytogenes in 75 samples (72.1% of the total; 50.0% of the carcasses and 94.2% of the cuts). The 75 isolates, one for each positive sample, were tested for resistance against a panel of 15 antibiotics of clinical interest by the disc diffusion method. All isolates belonged to the serogroup IIa (multiplex PCR assay) and showed resistance to between four and ten antibiotics, with an average value of 5.7 ± 2.0 resistances per isolate, this rising to 7.0 ± 2.1 when strains with resistance and reduced susceptibility were taken together. A high prevalence of resistance was observed for antibiotics belonging to the cephalosporin and quinolone families. However, the level of resistance was low for antibiotics commonly used to treat listeriosis (e.g., ampicillin or gentamicin). Nine different resistance patterns were noted. One isolate with each resistance pattern was tested for its ability to form biofilms on polystyrene during 72 h at 12 °C. The total biovolume of the biofilms registered through confocal laser scanning microscopy (CLSM) in the observation field of 16,078.24 μm2 ranged between 13,967.7 ± 9065.0 μm3 and 33,478.0 ± 23,874.1 μm3, and the biovolume of inactivated bacteria between 0.5 ± 0.4 μm3 and 179.1 ± 327.6 μm3. A direct relationship between the level of resistance to antibiotics and the ability of L. monocytogenes strains to form biofilms is suggested.
On Traub–Steffensen-Type Iteration Schemes With and Without Memory: Fractal Analysis Using Basins of Attraction
Authors: Junjua Moin-ud-Din , Abdullah Shahid , Kansal Munish , Ahmad Shabbir
Published: November 2024

This paper investigates the design and stability of Traub–Steffensen-type iteration schemes with and without memory for solving nonlinear equations. Steffensen’s method overcomes the drawback of the derivative evaluation of Newton’s scheme, but it has, in general, smaller sets of initial guesses that converge to the desired root. Despite this drawback of Steffensen’s method, several researchers have developed higher-order iterative methods based on Steffensen’s scheme. Traub introduced a free parameter in Steffensen’s scheme to obtain the first parametric iteration method, which provides larger basins of attraction for specific values of the parameter. In this paper, we introduce a two-step derivative free fourth-order optimal iteration scheme based on Traub’s method by employing three free parameters and a weight function. We further extend it into a two-step eighth-order iteration scheme by means of memory with the help of suitable approximations of the involved parameters using Newton’s interpolation. The convergence analysis demonstrates that the proposed iteration scheme without memory has an order of convergence of 4, while its memory-based extension achieves an order of convergence of at least 7.993, attaining the efficiency index 7.9931/3≈2. Two special cases of the proposed iteration scheme are also presented. Notably, the proposed methods compete with any optimal j-point method without memory. We affirm the superiority of the proposed iteration schemes in terms of efficiency index, absolute error, computational order of convergence, basins of attraction, and CPU time using comparisons with several existing iterative methods of similar kinds across diverse nonlinear equations. In general, for the comparison of iterative schemes, the basins of iteration are investigated on simple polynomials of the form zn−1 in the complex plane. However, we investigate the stability and regions of convergence of the proposed iteration methods in comparison with some existing methods on a variety of nonlinear equations in terms of fractals of basins of attraction. The proposed iteration schemes generate the basins of attraction in less time with simple fractals and wider regions of convergence, confirming their stability and superiority in comparison with the existing methods.
Ascorbic Acid Ameliorates Molecular and Developmental Defects in Human-Induced Pluripotent Stem Cell and Cerebral Organoid Models of Fragile X Syndrome
Authors: Gunapala Keith M. , Gadban Aseel , Noreen Faiza , Schär Primo , Benvenisty Nissim , Taylor Verdon
Published: November 2024

Fragile X Syndrome (FX) is the most common form of inherited cognitive impairment and falls under the broader category of Autism Spectrum Disorders (ASD). FX is caused by a CGG trinucleotide repeat expansion in the non-coding region of the X-linked Fragile X Messenger Ribonucleoprotein 1 (FMR1) gene, leading to its hypermethylation and epigenetic silencing. Animal models of FX rely on the deletion of the Fmr1 gene, which fails to replicate the epigenetic silencing mechanism of the FMR1 gene observed in human patients. Human stem cells carrying FX repeat expansions have provided a better understanding of the basis of epigenetic silencing of FMR1. Previous studies have found that 5-Azacytidine (5Azac) can reverse this methylation; however, 5Azac can be toxic, which may limit its therapeutic potential. Here, we show that the dietary factor Ascorbic Acid (AsA) can reduce DNA methylation in the FMR1 locus and lead to an increase in FMR1 gene expression in FX iPSCs and cerebral organoids. In addition, AsA treatment rescued neuronal gene expression and morphological defects observed in FX iPSC-derived cerebral organoids. Hence, we demonstrate that the dietary co-factor AsA can partially revert the molecular and morphological defects seen in human FX models in vitro. Our findings have implications for the development of novel therapies for FX in the future.
In Vivo Selection of S/MAR Sequences to Favour AAV Episomal Maintenance in Dividing Cells
Authors: Llanos-Ardaiz Andrea , Lantero Aquilino , Neri Leire , Mauleón Itsaso , Ruiz de Galarreta Marina , Trigueros-Motos Laia , Weber Nicholas D. , Ferrer Veronica , Aldabe Rafael , Gonzalez-Aseguinolaza Gloria
Published: November 2024

Adeno-associated viral (AAV) vector-mediated gene therapy has emerged as a promising alternative to liver transplantation for monogenic metabolic hepatic diseases. AAVs are non-integrative vectors that are maintained primarily as episomes in quiescent cells like adult hepatocytes. This quality, while advantageous from a safety perspective due to a decreased risk of insertional mutagenesis, becomes a disadvantage when treating dividing cells, as it inevitably leads to the loss of the therapeutic genome. This is a challenge for the treatment of hereditary liver diseases that manifest in childhood. One potential approach to avoid vector genome loss involves putting scaffold/matrix attachment regions (S/MARs) into the recombinant AAV (rAAV) genome to facilitate its replication together with the cellular genome. We found that the administration of AAVs carrying the human β-interferon S/MAR sequence to neonatal and infant mice resulted in the maintenance of higher levels of viral genomes. However, we also observed that its inclusion at the 3′ end of the mRNA negatively impacted its stability, leading to reduced mRNA and protein levels. This effect can be partially attenuated by incorporating nonsense-mediated decay (NMD)-inhibitory sequences into the S/MAR containing rAAV genome, whose introduction may aid in the development of more efficient and longer-lasting gene therapy rAAV vectors.
Energy Deficiency and Misdistribution Leads to Disrupted Formation in Grain Yield and Rice Quality
Authors: Wang Yiding , Li Guangyan , Ma Jiaying , Su Haoran , Hu Wenfei , Lin Junjiang , Fu Weimeng , Zeng Yvxiang , Tao Longxing , Fu Guanfu , Xiong Jie , Chen Tingting
Published: November 2024

With the progress of society and the improvement of agricultural scientific technology, the single focus on high yield for rice production has gradually shifted to high quality. Coordinated development of grain yield and rice quality has become a core issue for researchers, and the underlying mechanisms remain to be solved. Two varieties, Zhongzheyou1 (ZZY1) and Zhongzheyou8 (ZZY8), were used as study materials under field conditions. The yield of ZZY1 was higher than that of ZZY8, which was mainly characterized by a higher seed-setting rate and grain weight. The rice quality of ZZY8 was better than that of ZZY1, primarily due to lower chalkiness and a higher head rice rate. The total dry matter weight of ZZY1 was lower than that of ZZY8, but the proportion of panicle dry matter weight or nonstructural carbohydrate to the total in the former was higher than that of the latter. The maximum grain-filling rate, average grain-filling rate, and key enzyme activities of ZZY1 were significantly higher than those of ZZY8, while the active grain-filling period was shorter than that of ZZY8. Furthermore, the ATP/ATPase content and energy charge values in the grains of ZZY1 were higher than those of ZZY8 at the early grain-filling stage. Transcriptome analysis showed that carbohydrate and energy metabolism were the main ways affecting the yield and quality of the two varieties. The energy production of ZZY1 was insufficient to simultaneously supply the needs thus leading to the discordant formation in its grain yield and rice quality formation.
Action Mechanisms of Exosomes Derived from GD3/GD2-Positive Glioma Cells in the Regulation of Phenotypes and Intracellular Signaling: Roles of Integrins
Authors: Hasnat Mohammad Abul , Ohmi Yuhsuke , Yesmin Farhana , Kaneko Kei , Kambe Mariko , Kitaura Yoko , Ito Takako , Imao Yuka , Kano Keiko , Mishiro-Sato Emi , Koyanagi Hiroka , Kawamoto Yoshiyuki , Bhuiyan Robiul Hasan , Ohkawa Yuki , Tajima Orie , Furukawa Koichi , Furukawa Keiko
Published: November 2024

Extracellular vesicles (EVs) play important roles in intercellular communication in various biological events. In particular, EVs released from cancer cells have attracted special attention. Although it has been reported that cancer-associated glycosphingolipids play important roles in the enhancement of malignant properties of cancer cells, the presence, behavior, and roles of glycosphingolipids in EVs have not been elucidated. Recently, we reported crucial roles of EVs expressing gangliosides, GD2, and/or GD3 in the enhancement of cancer properties in malignant melanomas and gliomas. However, how EVs containing cancer-associated glycosphingolipids play their roles has not been reported to date. Here, we studied spatio-temporal mechanisms for GD3/GD2-containing EVs released from gliomas in the actions toward target cells. Proteome analyses of EVs with/without GD3/GD2 revealed an equally high concentration of integrin isoforms in both GD3/GD2+ and GD3/GD2- EVs. PKH26-labeled EVs attached, invaded, and distributed to/in the target cells within 1 h. GD3/GD2 formed molecular complexes with integrins on EVs as elucidated by immunoprecipitation/immunoblotting and immunocytostaining. The addition of antibodies reactive with GD3, GD2, or integrins resulted in the suppression of the enhancing effects of EVs in the cell adhesion assay. The addition of GD3/GD2 + EVs to GD3/GD2- cells clearly increased the phosphorylation levels of the PDGF receptor, FAK, and Erk1/2 in immunoblotting, suggesting GD3/GD2+ EVs activate the signaling pathway in the target cells within 15 min after addition. Anti-ganglioside antibodies clearly blocked signaling with EVs. In conclusion, EVs released from GD3/GD2-expressing glioma cells enhance cancer phenotypes and malignant signals via the cluster formation of integrins and GD3/GD2 on EVs, leading to the regulation of the cancer microenvironment.
Genome-Wide Identification of the Lectin Receptor-like Kinase Gene Family in Avena sativa and Its Role in Salt Stress Tolerance
Authors: Xiong Gui , Cui Dongli , Tian Yaqi , Schwarzacher Trude , Heslop-Harrison John Seymour , Liu Qing
Published: November 2024

Lectin receptor-like kinases (LecRLKs) are membrane-bound receptor genes found in many plant species. They are involved in perceiving stresses and responding to the environment. Oat (Avena sativa; 2n = 6x = 42) are an important food and forage crop with potential in drought, saline, or alkaline soils. Here, we present a comprehensive genome-wide analysis of the LecRLK gene family in A. sativa and the crop’s wild relatives A. insularis (4x) and A. longiglumis (2x), unveiling a rich diversity with a total of 390 LecRLK genes identified, comprising 219 G-types, 168 L-types, and 3 C-types in oats. Genes were unevenly distributed across the oat chromosomes. GFP constructs show that family members were predominantly located in the plasma membrane. Expression under salt stress demonstrated functional redundancy and differential expression of LecRLK gene family members in oats: 173 members of this family were involved in the response to salt stress, and the expression levels of three C-type genes in the root and leaf were significantly increased under salt stress. The results show the diversity, evolutionary dynamics, and functional implications of the LecRLK gene family in A. sativa, setting a foundation for defining its roles in plant development and stress resilience, and suggesting its potential agricultural application for crop improvement.
Degradation and/or Dissociation of Neurodegenerative Disease-Related Factor Amyloid-β by a Suspension Containing Calcium Hydrogen Carbonate Mesoscopic Crystals
Authors: Iwaya Nodoka , Sakudo Akikazu , Kanda Takuya , Furusaki Koichi , Onishi Rumiko , Onodera Takashi , Yoshikawa Yasuhiro
Published: November 2024

Amyloid-β (Aβ) aggregates accumulate in the brains of individuals with Alzheimer’s disease and are thought to potentially act as prions, promoting further aggregation. Consequently, the biochemistry of Aβ has emerged as a promising target for Alzheimer’s disease. CAC-717, a suspension of calcium bicarbonate mesoscopic structures derived from natural sources, has been shown to inactivate various pathogens, including prions. This study examined the effects of CAC-717 on both the formation and degradation/dissociation of Aβ aggregates using thioflavin T fluorescence and enzyme-linked immunosorbent assays. Aggregates of Aβ(1–42) peptide were generated by incubation at 37 °C for 24 h, and the effect of introducing CAC-717 on the aggregates was evaluated after further incubation at 25 °C for 30 min. Moreover, CAC-717 was also tested for its ability to inhibit the initial aggregation of Aβ. The results showed that CAC-717 significantly degraded and/or dissociated Aβ aggregates in a concentration-dependent manner. Specifically, CAC-717 treatment for 5 min disrupted Aβ aggregates to give Aβ monomer and oligomer concentrations as high as 130 nM compared to ~10 nM for the water control. In addition, CAC-717 degraded and/or dissociated aggregates within 10 s at 37 °C, and pre-treatment with CAC-717 significantly inhibited aggregation. These results suggest that CAC-717 not only degrades and/or dissociates Aβ aggregates but also inhibits their formation, highlighting its potential as a disinfectant for Alzheimer’s disease.
Improved Skin Barrier Function Along with Hydration Benefits of Viola yedoensis Extract, Aesculin, and Schaftoside and LC-HRMS/MS Dereplication of Its Bio-Active Components
Authors: Thonthula Sreelatha , Sousa Sandra De , Dubuis Alexis , Boudah Samia , Mehta Richa , Singh Akanksha , Eilstein Joan , Tabet Jean-Claude , John Sherluck , Roy Dhimoy , Pannakal Steve Thomas
Published: November 2024

The skin hydration level is a key factor that influences the physical and mechanical properties of the skin. The stratum corneum (SC), the outermost layer of the epidermis, is responsible for the skin’s barrier function. In this study, we investigated the role of a unique composition of Viola yedoensis extract for its ability to activate CD44, a cell-surface receptor of hyaluronic acid, and aquaporin-3, a water-transporting protein, in human keratinocytes (HaCaT). An ELISA assay evaluating the protein expression levels of CD44, aquaporin-3 (AQP3), filaggrin, and keratin-10 revealed that V. yedoensis extract upregulated the levels of CD44 and AQP3 by 15% and 78%, respectively. Additionally, V. yedoensis extract demonstrated a comparative effect on water vapor flux in TEWL and lipid perturbation in DSC versus the reference, glycerin. In light of this new biological efficacy, a detailed phytochemical characterization was undertaken using an integrated LC-HRMS/MS-based metabolomics approach, which provided further insights on the chemistry of V. yedoensis. This led to the identification of 29 secondary metabolites, 14 of which are reported here for the first time, including esculetin, aesculin, apigenin and kaempferol C-glycosides, megastigmane glycosides, roseoside, platanionoside B, and an eriojaposide B isomer, along with the rare, calenduloside F and esculetin diglucoside, which are reported for the first time from the genus, Viola. Notably, two active components identified in the V. yedoensis extract, namely, aesculin and schaftoside, showed an upregulation of the protein expression of CD44 in HaCaT cells by 123% and 193% within 24 h of treatment, respectively, while aesculin increased AQP3 levels by 46%. Aesculin and schaftoside also significantly upregulated the expression of K-10 levels by 299% and 116%, which was considerably higher than sodium hyaluronate, the positive control. The rationale used to characterize the new structures is outlined along with the related biosynthetic pathways envisioned to generate roseoside and Eriojaposide B. These findings provide new molecular insights to deepen the understanding of how V. yedoensis extract, along with the biomarkers aesculin and schaftoside, restores the skin barrier and skin hydration benefits.
WordDGA: Hybrid Knowledge-Based Word-Level Domain Names Against DGA Classifiers and Adversarial DGAs
Authors: Selvaraj Sarojini , Panjanathan Rukmani
Published: November 2024

A Domain Generation Algorithm (DGA) employs botnets to generate domain names through a communication link between the C&C server and the bots. A DGA can generate pseudo-random AGDs (algorithmically generated domains) regularly, a handy method for detecting bots on the C&C server. Unlike current DGA detection methods, AGDs can be identified with lightweight, promising technology. DGAs can prolong the life of a viral operation, improving its profitability. Recent research on the sensitivity of deep learning to various adversarial DGAs has sought to enhance DGA detection techniques. They have character- and word-level classifiers; hybrid-level classifiers may detect and classify AGDs generated by DGAs, significantly diminishing the effectiveness of DGA classifiers. This work introduces WordDGA, a hybrid RCNN-BiLSTM-based adversarial DGA with strong anti-detection capabilities based on NLP and cWGAN, which offers word- and hybrid-level evasion techniques. It initially models the semantic relationships between benign and DGA domains by constructing a prediction model with a hybrid RCNN-BiLSTM network. To optimize the similarity between benign and DGA domain names, it modifies phrases from each input domain using the prediction model to detect DGA family categorizations. The experimental results reveal that dodging numerous wordlists and mixed-level DGA classifiers with training and testing sets improves word repetition rate, domain collision rate, attack success rate, and detection rate, indicating the usefulness of cWGAN-based oversampling in the face of adversarial DGAs.
Small Diameter (7.5 Fr) Single-Use Flexible Ureteroscopy with Direct In-Scope Suction (DISSTM) in Conjunction with Aspiration-Assisted Flexible Access Sheath: A New Hype for Real Stone-Free?
Authors: Geavlete Petrișor , Mareș Cristian , Mulțescu Răzvan , Georgescu Dragoș , Ene Cosmin-Victor , Iordache Valentin , Geavlete Bogdan
Published: November 2024

Background: Renal lithiasis continues to represent a great challenge for modern urology in terms of minimally invasive management of lithotripsy procedures. The recent revolution of endoscopes with the miniaturization of instruments and the development of improved disposable ureteroscopes combined with recent suction methods such as suction access sheaths or direct in-scope suction (DISSTM) systems promise to further improve the outcome of renal stone treatment. Methods: Considering this technological advance, this study aims to evaluate the results obtained by combining these methods in three groups: Group 1—Single-use 7.5 Fr flexible ureteroscope with standard access sheath, Group 2—Single-use 7.5 Fr flexible ureteroscope with direct in-scope suction (DISSTM) with standard access sheath, Group 3—Single-use 7.5 Fr flexible ureteroscope with DISSTM with suction access sheath. A total number of 105 consecutive patients were enrolled in the study, divided equally in each group. Several parameters were followed, such as size and location of the stones, operative time, duration of hospitalization, the need for ureteral stent insertion, residual fragments, and subjective evaluation of the lithotripsy environment visualization. Results: The best results were highlighted in Group 3 for almost all evaluated parameters. Significant improvements were indicated in terms of stone-free rates. Conclusions: Additional large studies are needed to support these initial results, but preliminary data indicate a valuable advantage for every urologist who treats urolithiasis.
Novel Strategies for Lung Cancer Interventional Diagnostics
Authors: Smyth Robert , Billatos Ehab
Published: November 2024

Lung cancer is a major global health issue, with 2.21 million cases and 1.80 million deaths reported in 2020. It is the leading cause of cancer death worldwide. Most lung cancers have been linked to tobacco use, with changes in cigarette composition over the years contributing to shifts in cancer types and tumor locations within the lungs. Additionally, there is a growing incidence of lung cancer among never-smokers, particularly in East Asia, which is expected to increase the global burden of the disease. The classification of non-small cell lung cancer (NSCLC) into distinct subtypes is crucial for treatment efficacy and patient safety, especially as different subtypes respond differently to chemotherapy. For instance, certain chemotherapeutic agents are more effective for adenocarcinoma than for squamous carcinoma, which has led to the exclusion of squamous carcinoma from treatments like Bevacizumab due to safety concerns. This necessitates accurate histological diagnosis, which requires sufficient tissue samples from biopsies. However, acquiring adequate tissue is challenging due to the complex nature of lung tumors, patient comorbidities, and potential complications from biopsy procedures, such as bleeding, pneumothorax, and the purported risk of local recurrence. The need for improved diagnostic techniques has led to the development of advanced technologies like electromagnetic navigation bronchoscopy (ENB), radial endobronchial ultrasound (rEBUS), and robotic bronchoscopy. ENB and rEBUS have enhanced the accuracy and safety of lung biopsies, particularly for peripheral lesions, but both have limitations, such as the dependency on the presence of a bronchus sign. Robotic bronchoscopy, which builds on ENB, offers greater maneuverability and stability, improving diagnostic yields. Additionally, new imaging adjuncts, such as Cone Beam Computed Tomography (CBCT) and augmented fluoroscopy, further enhance the precision of these procedures by providing real-time, high-resolution imaging. These advancements are crucial as lung cancer is increasingly being detected at earlier stages due to screening programs, which require minimally invasive, accurate diagnostic methods to improve patient outcomes. This review aims to provide a comprehensive overview of the current challenges in lung cancer diagnostics and the innovative technological advancements in this rapidly evolving field, which represents an increasingly exciting career path for aspiring pulmonologists.
Assessing Fat Grafting in Breast Surgery: A Narrative Review of Evaluation Techniques
Authors: Bogdan Razvan-George , Helgiu Alina , Cimpean Anca-Maria , Ichim Cristian , Todor Samuel Bogdan , Iliescu-Glaja Mihai , Bodea Ioan Catalin , Crainiceanu Zorin Petrisor
Published: November 2024

Fat grafting has gained prominence in reconstructive and aesthetic surgery, necessitating accurate assessment methods for evaluating graft volume retention. This paper reviews various techniques for assessing fat and fat grafts, including their benefits and limitations. Three-dimensional (3D) scanning offers highly accurate, non-invasive volumetric assessments with minimal interference from breathing patterns. Magnetic resonance imaging (MRI) is recognized as the gold standard, providing precise volumetric evaluations and sensitivity to complications like oil cysts and necrosis. Computed tomography (CT) is useful for fat volume assessment but may overestimate retention rates. Ultrasonography presents a reliable, non-invasive method for measuring subcutaneous fat thickness. Other methods, such as digital imaging, histological analysis, and weight estimation, contribute to fat graft quantification. The integration of these methodologies is essential for advancing fat graft assessment, promoting standardized practices, and improving patient outcomes in clinical settings.
Thermal Changes During Clavicle Fracture Healing in Children
Authors: Jurić Filip , Antabak Anko , Žgaljardić Ivonne , Bosak Veršić Ana , Sršen Medančić Suzana , Augustin Goran
Published: November 2024

Introduction: Clavicle fractures are among the most common in children, typically treated conservatively, with standard radiographs used to diagnose and monitor healing. Recently, infrared thermography (IRT) has been proposed as an alternative method for fracture detection, but no study has correlated the temperature changes during callus formation. Materials and Methods: Children aged 4–18 with X-ray-diagnosed clavicle fractures were included in the study. IRT measured temperatures above the fracture and contralateral healthy side on the 1st, 4th, 8th, 15th, and 22nd day after the injury. Along with IRT, an ultrasound was used to assess callus formation. Results: The study included 27 patients with an average age of 12.4 years, mostly boys. The left side was more often affected than the right side (33%). We found a correlation between callus formation and the ∆T. A maximum temperature difference of an average of 0.7 °C was noted during the proliferative phase of callus formation. After the formation of the fibrocartilaginous callus (4th to 8th day), the temperature above the fracture declined until it was equal (22nd day) to that of the healthy side. The average temperature difference between the broken and the healthy sides was statistically significant on the 4th and 8th days (during callus formation). Conclusions: The increased skin temperature above the fracture correlates with the inflammatory phase of bone healing. After the callus is visible on ultrasound, the temperature linearly drops with no statistical difference between the injured and the healthy sides. The standard protocol for clavicle fracture treatment typically involves using X-rays to assess callus formation during follow-up. IRT has shown potential in diagnosing callus formation in children with clavicle fractures, potentially reducing the need for traditional X-rays.
Temporoparietal Fascia Flap (TPFF) in Extended Endoscopic Transnasal Skull Base Surgery: Clinical Experience and Systematic Literature Review
Authors: Offi Martina , Mattogno Pier Paolo , D’Onofrio Ginevra Federica , Serioli Simona , Valeri Federico , Della Pepa Giuseppe Maria , Arena Vincenzo , Parrilla Claudio , Chiloiro Sabrina , D’Argento Francesco , Gessi Marco , Pedicelli Alessandro , Lauretti Liverana , Paludetti Gaetano , Galli Jacopo , Olivi Alessandro , Rigante Mario , Doglietto Francesco
Published: November 2024

Background and Objectives: The temporoparietal fascia flap (TPFF) has recently emerged as an option for skull base reconstruction in endoscopic transnasal surgery when vascularized nasal flaps are not available. This study provides a systematic literature review of its use in skull base surgery and describes a novel cohort of patients. Methods: PRISMA guidelines were used for the review. Patients undergoing skull base reconstruction with TPFF in our center from May 2022 to April 2024 were retrospectively included. Data were collected on pre- and post-operative clinical and radiological features, histology, surgical procedures, and complications. Results: Sixteen articles were selected, comprising 42 patients who underwent TPFF reconstruction for treatment of complex skull base pathologies. In total, 5 of 358 patients (0.9%) who underwent tumor resection via endoscopic transanal surgery in the study period in our institution required TPFF. All had been previously treated with surgery and radiation therapy for different pathologies (three chordomas, one giant pituitary neuroendocrine tumor (PitNET), and one sarcoma). Post-operative complications included CSF leak, which resolved after flap revision, and an internal carotid artery pseudoaneurysm requiring endovascular embolization. Conclusions: TPFF is an effective option for skull base reconstruction in complex cases and should be part of the armamentarium of the skull base surgeon.
Fabrication and Microstructure Analysis of Phosphate-Coated Mg Powder for Biomedical PLA/Mg Composites
Authors: Huang Ying-Ting , Hung Fei-Yi
Published: November 2024

Powdered magnesium has been widely applied in various fields. Magnesium is a highly reactive metal, with fine particles that are easy to agglomerate and have the risk of explosion. Furthermore, the storage of Mg particles is a challenge. Therefore, powdered magnesium is usually passivated by surface modification methods. In our research, an environmentally friendly phosphate solution was used to prepare conversion coating on magnesium particles. The results demonstrated that the phosphate coating layer attached on Mg particles surface successfully. From SEM images, the average particle size reduces slightly after the coating process. The composition of the coating layer is confirmed to be OCP and HAp by XRD and EPMA. The immersion test showed that the phosphate coating improved the corrosion resistance, and the ideal processing time is 20 min. Moreover, Mg and phosphate have good biocompatibility; therefore, the coated Mg powder can be a potential candidate for biomedical applications.
Obstacle Avoidance of Surface Agent Formation Based on Streamline Traction at Fixed-Time
Authors: Liu Yiping , Niu Yameng , Zhang Jianqiang , Tao Weihao
Published: November 2024

The marine environment is highly complex and variable, featuring obstacles such as islands, buoys, and vessels. Safe navigation of the surface agent (SA) fleet is crucial for ensuring the safety of the SA fleet, enhancing operational efficiency, and guaranteeing the smooth execution of the fleet’s mission. Regarding the problem of formation obstacle avoidance for SA fleets encountering complex obstacles during navigation, this chapter presents a fixed-time-based safe navigation algorithm for the SA fleet based on streamline traction. Firstly, to precisely position each SA at the designated location within the formation, a highly malleable leader–follower formation mode is introduced. Based on an enhanced interfered fluid dynamical system (EIFDS) obstacle avoidance algorithm, the virtual Leader is guided to evade static obstacles and determine a trajectory of the designated position. Secondly, a first-order fixed-time control Lyapunov function (FTCLF) is designed based on the EIFDS obstacle avoidance algorithm to guide the angular velocity constraint. The optimal guiding angular velocity signal is obtained through quadratic programming, ensuring that the SA steers towards the designated position while avoiding obstacles. Next, for the guiding velocity amplitude signal, a first-order fixed-time control barrier function (FTCBF) is designed based on the streamline formation scheme and the inter-boat safety distance to guide the velocity amplitude constraint. The optimal guiding velocity amplitude signal is obtained through quadratic programming, guaranteeing that each SA maintains the formation while avoiding collisions with adjacent vessels. Finally, the simulation results indicate the effectiveness, superiority, and stability of the proposed fixed-time-based safe navigation guidance algorithm for the SA fleet based on streamline traction.
Exploring the Impact of Land Certification on Centralized Transfer in Rural China: The Roles of Timing, Inequality, and Governance
Authors: Pan Lan , Wan Haoran , Cui Xufeng
Published: November 2024

The existing studies have primarily discussed the impact of land certification on farmers’ land transfer behaviors, neglecting its potential for centralized transfer under the orientation of optimizing land utilization and allocation efficiency. This study employed the Probit model, Propensity Score Matching method, and IV-Probit model to explore the effects and underlying mechanisms of land certification on centralized transfer based on the China Land Economic Survey data, especially emphasizing the roles of timing, inequality, and governance. Among transfer-out households, land certification increases the likelihood of farmers adopting centralized transfer rather than decentralized transfer. After considering the differential influence exerted by the timing of certification, the earlier the households obtain the land contract management certificates, the greater the positive impact. This phenomenon can be explained from the dual perspectives of economy and governance, including rural industrial development and rural governance performance. In the heterogeneity analysis, we focused on land inequality, farmers’ risk preference, and village governance teams’ capacity. Land certification mainly benefits farmers facing weak land inequality or low-risk preference, as well as those in villages with highly educated cadres. In the new round of land certification program, our findings provide new insights for continuously optimizing land utilization and allocation.
Integrating Multi-Source Urban Data with Interpretable Machine Learning for Uncovering the Multidimensional Drivers of Urban Vitality
Authors: Xie Yuchen , Zhang Jiaxin , Li Yunqin , Zhu Zehong , Deng Junye , Li Zhixiu
Published: November 2024

The complexity of urban street vitality is reflected in the interaction of multiple factors. A deep understanding of the multi-dimensional driving mechanisms behind it is crucial to enhancing urban street vitality. However, existing studies lack comprehensive interpretative analyses of urban multi-source data, making it difficult to uncover these drivers’ nonlinear relationships and interaction effects fully. This study introduces an interpretable machine learning framework, using Nanchang, China as a case study. It utilizes urban multi-source data to explore how these variables influence different dimensions of street vitality. This study’s innovation lies in employing an integrated measurement approach which reveals the complex nonlinearities and interaction effects between data, providing a more comprehensive explanation. The results not only demonstrate the strong explanatory power of the measurement approach but also reveal that (1) built environment indicators play a key role in influencing street vitality, showing significant spatial positive correlations; (2) different dimensions of street vitality exhibit nonlinear characteristics, with transit station density being the most influential one; and (3) cluster analysis revealed distinct built environment and socioeconomic characteristics across various street vitality types. This study provides urban planners with a data-driven quantitative tool to help formulate more effective strategies for enhancing street vitality.