bluuebunny/crossref_metadata_2025 huggingface dataset:
Safe Path Planning Method Based on Collision Prediction for Robotic Roadheader in Narrow Tunnels
Authors: Zhang Chao , Zhang Xuhui , Yang Wenjuan , Zhang Guangming , Wan Jicheng , Lei Mengyu , Dong Zheng
Published: February 2025

Safe path planning is essential for the autonomous operation of robotic roadheader in narrow underground tunnels, where limited perception and the robot’s geometric constraints present significant challenges. Traditional path planning methods often fail to address these issues. This paper proposes a collision prediction-integrated path planning method tailored for robotic roadheader in confined environments. The method comprises two components: collision prediction and path planning. A collision prediction model based on artificial potential fields is developed, considering the non-convex shape of the roadheader and enhancing scalability. By utilizing tunnel design information, a composite potential field model is created for both obstacles and the roadheader, enabling real-time collision forecasting. The A* algorithm is modified to incorporate the robot’s motion constraints, using a segmented weighted heuristic function based on collision predictions. Path smoothness is achieved through Bézier curve smoothing. Experimental results in both obstacle-free and obstacle-laden scenarios show that the proposed method outperforms traditional approaches in terms of computational efficiency, path length, and smoothness, ensuring safe, efficient navigation in narrow tunnels.
A Group Consensus Measure That Takes into Account the Relative Importance of the Decision-Makers
Authors: Dombi József , Fáró Jenő , Jónás Tamás
Published: February 2025

In group decision making, the knowledge, skills, and experience of the decision-makers may not be at the same level. Hence, the need arises to take into account not only the opinion, but also the relative importance of the opinion of each decision-maker. These relative importance values can be treated as weights. In a group decision making situation, it is not only the weighted aggregate output that matters, but also the weighted measure of the group consensus. Noting that weighted group consensus measures have not yet been intensely studied, in this study, based on well-known requirements for non-weighted consensus measures, we define six reasonable requirements for the weighted case. Then, we propose a function family and prove that it satisfies the above requirements for a weighted consensus measure. Hence, the proposed measure can be used in group decision making situations where the decision-makers have various weight values that reflect the relative importance of their opinions. The proposed weighted consensus measure is based on the fuzziness degree of the decumulative distribution function of the input scores, taking into account the weights. Hence, it may be viewed as a weighted adaptation of the so-called fuzziness measure-based consensus measure. The novel weighted consensus measure is determined by a fuzzy entropy function; i.e., this function may be regarded as a generator of the consensus measure. This property of the proposed weighted consensus measure family makes it very versatile and flexible. The nice properties of the proposed weighted consensus measure family are demonstrated by means of concrete numerical examples.
Evaluation of Risk Factors and a Gene Panel as a Tool for Unexplained Infertility Diagnosis by Next-Generation Sequencing
Authors: Jašinskienė Eglė , Sniečkutė Ieva , Galminas Ignas , Žemaitis Lukas , Simutis Mantas , Čaplinskienė Marija
Published: February 2025

Background and Objective: Unexplained infertility is a major challenge in reproductive medicine and requires advanced diagnostic approaches to identify the underlying factors accurately. This study aims to evaluate the utility of risk factor analysis and a gene panel in diagnosing unexplained infertility using the next-generation sequencing (NGS) technology. Our study aimed to characterize and identify risk and genetic factors associated with unexplained infertility. Materials and methods: A cohort of patients with unexplained infertility was comprehensively screened for risk factors and genetic variations using a targeted gene panel (10 couples with unexplained infertility (UI) and 36 fertile couples). 108 articles were selected (58 on female infertility and 50 on male infertility) presenting genes that may be associated with unexplained infertility. A gene panel for unexplained infertility was compiled based on the literature data. A customized virtual panel was created from the exome sequencing data. Results: In the female group, controls had a higher mean age, while in the male patients, both groups were similar in terms of age. Both gender groups had comparable BMI values. No significant associations (p > 0.05) between risk factors and unexplained infertility were found when evaluating anthropometric parameters and other sociodemographic characteristics. In two male patients (20%), a molecular defect was detected in NGS variants classified aspossible benign and probably benign In particular, missense variants were identified in the UGT2B7 and CATSPER2 genes, A molecular defect classified as probably damaging was found in five female patients (50%). In particular, missense variants were identified in the CAPN10, MLH3, HABP2, IRS1, GDF9, and SLC19A1 genes. Conclusions: The study emphasizes that unexplained infertility is often related to mechanisms beyond causative mutations and highlights the need for integrative genomic research involving broader gene panels and multi-faceted approaches, including transcriptomics and epigenetics, to uncover latent genetic predispositions.
Removal of Microplastics from Laundry Wastewater Using Coagulation and Membrane Combination: A Laboratory-Scale Study
Authors: Luu Thi Trang , Truong Dai Quyet , Nguyen Van Nam , Jeong Sanghyun , Nguyen Thi Thu Trang , Do Van Manh , Vigneswaran Saravanamuthu , Nguyen Tien Vinh
Published: February 2025

Microplastic (MP) pollution has recently emerged as a critical global environmental issue. Laundry wastewater is a significant contributor to MP pollution, containing high concentrations of MPs. Although coagulation has recently been widely applied to remove MPs from such wastewater, its efficiency remains poor, and the removal mechanisms are not yet fully elucidated. In this study, the occurrence and characteristics of MPs in raw domestic laundry wastewater were investigated. The coagulation process was combined with ultrafiltration (UF) membrane filtration to enhance MP removal. The results showed that the concentrations of MPs in laundry wastewater ranged from 9000 to 11,000 particles/L, with fibrous particles constituting the majority (42.6%) and polyester accounting for 68.2% of detected MPs. Using aluminium chloride and ferric chloride as coagulants, maximum removal efficiencies of 91.7 and 98.3% were achieved, respectively. Mechanistic analysis revealed that charge neutralization played a dominant role during coagulation. Fourier transform infrared spectroscopy further demonstrated the formation of new functional groups, substituted benzene rings, and the presence of Fe-O and Al-O bonds, indicating the interaction between MPs and coagulants. Furthermore, the UF membrane was used to remove fibrous MPs and MPs with low densities. These MPs had not been removed with pre-coagulation. The removal efficiency of these MPs reached 96 ± 2%, reducing their concentration to only 60 particles/L in the UF permeate. These findings highlight the synergistic potential of coagulation and UF membrane filtration for effective MP removal and provide a valuable reference for advancing wastewater treatment technologies targeting MP pollution.
Metabolomic-Based Assessment of Earthworm (Eisenia fetida) Exposure to Different Petroleum Fractions in Soils
Authors: Liu Meiyu , Wang Mutian , Fu Xiaowen , Song Fanyong , Zhou Fangyuan , Li Tianyuan , Wang Jianing
Published: February 2025

Background/Objectives: Petroleum contamination in soil exerts toxic effects on earthworms (Eisenia fetida) through non-polar narcotic mechanisms. However, the specific toxicities of individual petroleum components remain insufficiently understood. Methods: This study investigates the effects of four petroleum components—saturated hydrocarbons, aromatic hydrocarbons, resins, and asphaltenes—on earthworms in artificially contaminated soil, utilizing a combination of biochemical biomarker analysis and metabolomics to uncover the underlying molecular mechanisms. Results: The results revealed that aromatic hydrocarbons are the most toxic fraction, with EC50 concentrations significantly lower than those of other petroleum fractions. All tested fractions triggered notable metabolic disturbances and immune responses in earthworms after 7 days of exposure, as evidenced by significant changes in metabolite abundance within critical pathways such as arginine synthesis, a-linolenic acid metabolism, and the pentose phosphate pathway. According to the KEGG pathway analysis, saturated hydrocarbon fractions induced marked changes in glycerophospholipid metabolism, and arginine and proline metabolism pathways, contributing to the stabilization of the protein structure and membrane integrity. Aromatic hydrocarbon fractions disrupted the arachidonic acid metabolic pathway, leading to increased myotube production and enhanced immune defense mechanisms. The TCA cycle and riboflavin metabolic pathway were significantly altered during exposure to the colloidal fraction, affecting energy production and cellular respiration. The asphaltene fraction significantly impacted glycolysis, accelerating energy cycling to meet stress-induced increases in energy demands. Conclusions: Aromatic hydrocarbons accounted for the highest level of toxicity among the four components in petroleum-contaminated soils. However, the contributions of other fractions to overall toxicity should not be ignored, as each fraction uniquely affects key metabolic pathways and biological functions. These findings emphasize the importance of monitoring metabolic perturbations caused by petroleum components in non-target organisms such as earthworms. They also reveal the specificity of the toxic metabolic effects of different petroleum components on earthworms.
Practical Recommendations in the Treatment of Acute and Chronic Life-Threatening Infectious Diseases in Patients with Acute Hepatic Porphyria
Authors: Badia Bruno de Mattos Lombardi , Serrano Paulo de Lima , Barile João Paulo , Seneor Daniel Delgado , Mendes Patrícia Marques , Cavalheiro Renan Brandão Rambaldi , Peixoto Kaliny Oliveira , Farias Igor Braga , Machado Roberta Ismael Lacerda , Pinto Wladimir Bocca Vieira de Rezende , Oliveira Acary Souza Bulle , Sgobbi Paulo
Published: February 2025

Background: Acute hepatic porphyrias (AHPs) represent inherited metabolic disorders of the heme biosynthesis pathway, leading to neurological and systemic impairment. Despite the presence of well-recognized chronic symptoms and signs, acute neurological, both neuromuscular and central neurological complications pose a significant challenge in clinical practice, with a potential risk of greater severity and mortality during acute decompensation episodes of AHPs. Care related to the prescription of medications, considering the risk of porphyrinogenicity, is a major and recurring concern in the acute and chronic management of AHP patients. Infectious clinical complications are significant issues in both outpatient and hospital settings for patients with AHPs. It is crucial to identify therapeutic regimens with the best safety and efficacy profiles for treating such infectious complications in AHP patients. The scarcity of structured knowledge available in guidelines and recommendations often leads to the use of therapeutic options with higher potential risks in treating patients with AHPs. Objectives: This review article aims to provide practical recommendations for managing the most significant infectious complications in clinical practice, with a focus on their impact on the clinical care of patients with AHPs.
Mixotrophy in Marine Microalgae to Enhance Their Bioactivity
Authors: Licata Gabriella , Galasso Christian , Palma Esposito Fortunato , Palumbo Piccionello Antonio , Villanova Valeria
Published: February 2025

Photosynthetic microorganisms, such as microalgae, are remarkable for their ability to harness sunlight, fix carbon dioxide, and produce a variety of bioactive compounds. These organisms are pivotal in climate mitigation strategies as they can absorb carbon dioxide while generating valuable biomolecules. Among the diverse cultivation approaches, mixotrophic growth combines light energy with both inorganic and organic carbon sources, offering a unique strategy to enhance biomass production and metabolic diversity in microalgae. Here, microalgal species such as Nannochloropsis granulata, Phaeodactylum tricornutum, and Chlorella sp. were investigated for their potential applications under different cultivation methods, including phototrophy and mixotrophy. Mixotrophic conditions significantly improved biomass production across all tested species. Among these, Phaeodactylum tricornutum, a marine diatom, emerged as a promising candidate for bioactive compound production, exhibiting higher antiproliferative activity against human melanoma cells and antibacterial effects against Staphylococcus aureus. Importantly, Chlorella sp. was also found to possess antibacterial activity against Staphylococcus aureus, broadening its potential applications. Additionally, metabolomics analysis was performed on Chlorella sp. and Phaeodactylum tricornutum to identify the compounds responsible for the observed bioactivity. This study highlights the value of mixotrophic cultivation in enhancing the productivity and bioactivity of microalgae, positioning them as versatile organisms for sustainable biotechnological applications.
Host–Microbe Interactions in Healthy and CSOM-Affected Middle Ears
Authors: Neeff Michel , Kimita Wandia , Waldvogel-Thurlow Sharon , Douglas Richard G. , Biswas Kristi
Published: February 2025

Chronic suppurative otitis media (CSOM) is a chronic middle ear inflammatory condition due to persistent polymicrobial middle ear infection. The interaction between local immune responses and microbial communities is not well understood, complicating the development of targeted therapies. This study aimed to characterise local immune cell responses and microbial composition in CSOM-affected middle ear mucosa, focusing on Pseudomonas aeruginosa and Staphylococcus aureus. A total of 24 CSOM patients and 22 controls undergoing tympanomastoid surgery participated in this prospective study. Middle ear and mastoid mucosa were collected for histological and microbiological analysis. Bacterial identification was performed using standard culture methods and Vitek MS, while immune cell populations were quantified via immunohistochemistry. Statistical analyses were performed using Kruskal–Wallis and Mann–Whitney tests. Microbiology results identified multiple pathogens in CSOM, including S. aureus and P. aeruginosa, with polymicrobial infections in 10 samples. CSOM patients exhibited significantly elevated immune cells, including CD3+, CD20+, and CD68+ cells, compared to controls. Histological analysis showed Gram-positive bacteria in three mastoid samples, with positive antibody staining for S. aureus (20.8%) and P. aeruginosa (12.5%) in CSOM patients. Controls had no bacterial staining. Intracellular bacteria may evade host defences and reduce antibiotic efficacy, contributing to CSOM persistence. Targeting intracellular pathogens in future treatments, along with studying polymicrobial communities, could improve management strategies.
Cyto- and Genotoxicity of Selected Plant Extracts and Microbial Metabolites with Confirmed Activity Against Phytopathogens of Potato Seed (Solanum tuberosum L.)
Authors: Nowak Adriana , Steglińska Aleksandra , Gutarowska Beata , Kręgiel Dorota
Published: February 2025

The aim of this study was to evaluate the cytotoxicity and genotoxicity of potential biocontrol agents for use against phytopathogens of potato seed (Solanum tuberosum L.). Plant extracts from Allium sativum L., Syzygium aromaticum L. Merr. & Perry, Salvia officinalis L., and Curcuma longa L., as well as metabolites of bacteria Lactiplantibacillus plantarum KB2 LAB 03 and yeast Metschnikowia pulcherrima TK1, were investigated. The chemical characteristics of the plant extracts and the metabolic profiles of the tested microorganisms were evaluated by GC-MS. An insect cell line from Spodoptera frugiperda (Sf-9) and human cervix adenocarcinoma cells (HeLa) were used to evaluate cytotoxicity in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The single-cell electrophoresis assay was used to estimate DNA damage. The cytotoxicity and genotoxicity of the microbial metabolites depended on their chemical profiles and pH. The plant extracts induced stronger DNA damage in the Sf-9 cell line than in HeLa cells. The garlic (Allium sativum L.) extract showed the highest cytotoxicity against Sf-9 insect cells (IC50 41.6 mg/mL). The sage (Salvia officinalis L.) extract showed the highest cytotoxicity against HeLa cells (IC50 49.6 mg/mL). This study is the first to investigate not only the potential of these novel biocontrol agents for plant disease control, but also their safety for humans and biodiversity within the context of sustainable agriculture.
Photoluminescence and Stability of Dion–Jacobson Tin-Based Halide Perovskites with Different Spacer Cation Chain Length
Authors: Ali Muhammad Umair , Sun Wen Ting , Sergeev Aleksandr A. , Rehman Atta Ur , Wong Kam Sing , Djurišić Aleksandra B. , Popović Jasminka
Published: February 2025

Two-dimensional tin halide perovskites are of significant interest for light emitting applications. Here, we investigate the effect of organic cation A on the stability of different Dion–Jacobson tin-based halide perovskites. The ASnBr4 materials using diammonium cation A with shorter alkyl chains are found to exhibit improved stability, exhibiting dramatic stability difference between the most stable HDASnBr4, where HDA denotes 1,6-hexanediammonium, and two materials with 8- and 10-carbon alkyl chain ammonium cations. The HDASnBr4 powders were thermally stable at 100 °C in an argon environment but exhibited decreasing photoluminescence with time in ambient air at 100 °C. The sample degradation at 100 °C is accelerated compared to room temperature, but it proceeds along similar pathways, namely phase transformation followed by perovskite decomposition. Light emission from HDASnBr4 thin films could be further enhanced by methanol vapor treatment, and warm white emission with Commission Internationale de l’Eclairage (CIE) coordinates (0.37, 0.34) could be obtained by combining HDASnBr4 with a blue-emitting polymer film, while direct mixing of blue phosphor and HDASnBr4 powder yields white emission with CIE coordinates of (0.34, 0.32).
Innovative Syntheses and Reactivity of Propiolamidines
Authors: Ginés Carlos , Parra-Cadenas Blanca , Carrillo-Hermosilla Fernando , Elorriaga David
Published: February 2025

Polydentate ligands with nitrogen donor atoms have possibly given rise to the largest group of coordination complexes described. Among these ligands, amidinates represent a nitrogenated version of carboxylates and allow the formation of complexes with most elements in the periodic table, adopting chelate or bridge coordination modes. The precursors of these ligands, amidines, can present an alkynyl group as the substituent of their central atom, R′N=C(C≡CR)NHR′, which provides an additional point of reactivity for these molecules, as well as a different electronic behavior compared to conventional amidines with alkyl groups on the central carbon atom of the amidine group. These propiolamidines have been obtained through classical stoichiometric organic synthesis procedures or, with greater atomic economy, through catalytic procedures based on Main Group, Transition, or Rare Earth metals. This work reviews these synthesis methods, as well as the reactivity in the obtention of new, more complex heterocyclic organic molecules.
Characteristic Fragmentation Behavior of Linear and Cyclic O-Linked Glycopeptides and Their Peptide Skeletons in MALDI-TOF/TOF MS
Authors: Fukushi Kohki , Urakami Shogo , Hinou Hiroshi
Published: February 2025

Understanding characteristic post-source decay (PSD) fragmentation patterns in tandem mass spectrometry (MS/MS) is important for the identification of target molecules. In this study, we explored the characteristic PSD patterns associated with O-linked glycopeptides and their cyclization using the MALDI-TOF/TOF MS analysis of linear and cyclic antifreeze glycoproteins. We performed a comparative analysis of the proton and sodium adduct ions of the peptide backbones of antifreeze glycoproteins, which have a simple repeating sequence, shedding light on the characteristics of the fragmentation of the threonine side chain and that of its cyclized form. Furthermore, the presence or absence of a glycan on the threonine side chain and its substitution with serine caused changes in its fragmentation. These findings are expected to contribute to the prediction of three-dimensional peptide structures and the search for physiologically active O-linked glycopeptides and cyclic (glyco)peptides.
Screening Refractory Dye Degradation by Different Advanced Oxidation Processes
Authors: Ouagued Imane , Cretin Marc , Petit Eddy , Lesage Geoffroy , Djafer Abderrahmane , Ouagued Abdallah , Lacour Stella
Published: February 2025

This study investigated Rhodamine B (RhB) degradation by electro-Fenton (EF), anodic oxidation (AO), and their combination (EF/AO), using a carbon felt cathode coupled to a sub-stoichiometric titanium dioxide Magnéli phase (Ti4O7) anode or a platinized titanium (Ti/Pt) anode. The results indicated that operational parameters influenced the kinetics of electrochemical reactions. An increase in current density from 10 to 50 mA cm−2 significantly enhanced the RhB degradation rate; 30 mA cm−2 was the optimal current density, balancing both energy efficiency and degradation performance. Moreover, higher RhB concentrations required longer treatment. The Microtox® bioluminescence inhibition test revealed a significant toxicity decrease of the dye solution during electrochemical degradation, which was highest with EF/AO. Similarly, total organic carbon removal was highest with EF/AO (90% at pH 3), suggesting more efficient mineralization of RhB and its by-products than with EF or AO. Energy consumption remained relatively stable with all oxidation processes throughout the 480 min electrolysis period. High-resolution mass spectrometry elucidated RhB degradation pathways, highlighting chain oxidation reactions leading to the formation of intermediates and mineralization to CO2 and H2O. This study underscores the potential of EF, AO, and EF/AO as effective methods for RhB mineralization to develop sustainable and environmentally friendly wastewater treatment strategies.
Aldehyde-Assisted Alkoxysilane Condensation to Form Siloxane Bond: A New Process for Curing Alkoxy-Functional Silicone Resins
Authors: Rubinsztajn Sławomir , Mizerska Urszula , Kurjata Jan , Kwiatkowska Małgorzata , Cypryk Marek
Published: February 2025

The formation of the siloxane bond is one of the most important reactions used in silicone chemistry and technology. In this paper, a new process for the condensation of alkoxy-functional silanes to form a siloxane bond is presented. The new reaction is catalyzed by a Ge(II)+ complex stabilized by pentamethylcyclopentadiene with a weakly coordinated anion, tetrakis(pentafluorophenyl)borane. A mechanistic study of this new condensation process using model alkoxy-functional silanes and propionaldehyde is completed. It is established that the quantitative conversion of alkoxysilanes to the siloxane bond requires stoichiometric amounts of aldehyde. It is also found that paraldehyde can serve as a convenient, higher boiling source of acetaldehyde for the condensation of alkoxysilanes. The results obtained, supported by DFT calculations, allow for us to formulate the mechanism of this reaction.
Silk Sericin-Based Electrospun Nanofibers Forming Films for Cosmetic Applications: Preparation, Characterization, and Efficacy Evaluation
Authors: Dragojlov Ivana , Aad Rony , Ami Diletta , Mangiagalli Marco , Natalello Antonino , Vesentini Simone
Published: February 2025

This study investigates the extraction, characterization, and cosmetic application of silk sericin, a protein derived from Bombyx mori silkworm cocoons, with a focus on its potential in sustainable and biodegradable cosmetic formulations. Sericin was extracted using a high-temperature, high-pressure autoclave degumming method and spray-dried into a stable powder. The molecular weight distribution of sericin was analyzed, revealing fractions ranging from 10 to 37 kDa in Elution 1A and 25–40 kDa in Elution 1B. Electrospinning of sericin led to increased β-sheet content compared to raw sericin, as shown by secondary structure analyses. The electrospun sericin was then blended with gelatin to enhance mechanical strength and stability, resulting in robust films suitable for cosmetic applications. These films were developed into eye contour patches designed to deliver moisturizing, elasticizing, and smoothing effects. The efficacy of the patches was evaluated in 20 participants, showing increased skin elasticity (+35.1%) and smoothness (Ra: −30.7%, Rz: −26.6%), though a decline in hydration was observed, potentially indicating opportunities for further optimization.
Sam-Sam Association Between EphA2 and SASH1: In Silico Studies of Cancer-Linked Mutations
Authors: Vincenzi Marian , Mercurio Flavia Anna , Autiero Ida , Leone Marilisa
Published: February 2025

Recently, SASH1 has emerged as a novel protein interactor of a few Eph tyrosine kinase receptors like EphA2. These interactions involve the first N-terminal Sam (sterile alpha motif) domain of SASH1 (SASH1-Sam1) and the Sam domain of Eph receptors. Currently, the functional meaning of the SASH1-Sam1/EphA2-Sam complex is unknown, but EphA2 is a well-established and crucial player in cancer onset and progression. Thus, herein, to investigate a possible correlation between the formation of the SASH1-Sam1/EphA2-Sam complex and EphA2 activity in cancer, cancer-linked mutations in SASH1-Sam1 were deeply analyzed. Our research plan relied first on searching the COSMIC database for cancer-related SASH1 variants carrying missense mutations in the Sam1 domain and then, through a variety of bioinformatic tools and molecular dynamic simulations, studying how these mutations could affect the stability of SASH1-Sam1 alone, leading eventually to a defective fold. Next, through docking studies, with the support of AlphaFold2 structure predictions, we investigated if/how mutations in SASH1-Sam1 could affect binding to EphA2-Sam. Our study, apart from presenting a solid multistep research protocol to analyze structural consequences related to cancer-associated protein variants with the support of cutting-edge artificial intelligence tools, suggests a few mutations that could more likely modulate the interaction between SASH1-Sam1 and EphA2-Sam.
Research Progress in the Construction Strategy and Application of Superhydrophobic Wood
Authors: Chang Siyu , Yao Lihong , Wang Lei , Wu Yueqi
Published: February 2025

Wood serves as a green biomass material with sustainable utilization and environmental friendliness. The modification of wood can be used to obtain superhydrophobic properties and further expand wood’s application range. This paper focuses on the development status of superhydrophobic surfaces with micro-/nanoscale rough structures. Based on the surface wettability theory, this paper introduces common methods of superhydrophobic modification of wood materials, compares the advantages and disadvantages of these methods, discusses the relationship between the surface microstructure and wettability, and summarizes the applications of superhydrophobic wood in oil–water separation, self-cleaning, and self-healing. Finally, the future development strategies of superhydrophobic coating materials are elucidated to provide basic theoretical support for the synthesis and diverse applications of superhydrophobic wood and a reference for subsequent research and development.
Electromagnetic Nanocoils Based on InGaN Nanorings
Authors: Yan Ziwen , Chen Peng , Zhang Xianfei , Xie Zili , Xiu Xiangqian , Chen Dunjun , Zhao Hong , Shi Yi , Zhang Rong , Zheng Youdou
Published: February 2025

Energy issues, including energy generation, conversion, transmission and detection, are fundamental factors in all systems. In micro- and nanosystems, dealing with these energy issues requires novel nanostructures and precise technology. However, both concept and setup are not well established yet in the microsystems, especially for those at the nanometer scale. Here, we demonstrate electromagnetic nanocoils with 100 nm diameters based on uniform and periodic InGaN nanoring arrays grown on patterned GaN surfaces using nanoscale selective area epitaxy (NSAE). We observed stronger photoluminescence from the periodic InGaN nanoring arrays compared to the non-uniform InGaN nanorings, which indicates good crystal quality of the InGaN nanostructure with the NSAE. Based on this kind of nanostructure, electromagnetic induction from the nanorings is detected through the rebound movement of high-energy electron diffraction patterns that are influenced by a modulated external magnetic field. Our results clearly show the generation of an inductive current and internal magnetic field in the nanorings. We anticipate this kind of nanostructure to be a potential key element for energy conversion, transfer and detection in nanosystems. For example, it could be used to fabricate microtransformers and micro- and nanosensors for electromagnetic signals.
Remarkably High Dielectric Constant and Capacitance Density by Ni/ZrO2/TiN Using Nanosecond Laser and Surface Plasma Effect
Authors: Fan Wei Ting , Pooja Pheiroijam , Chin Albert
Published: February 2025

Rapid thermal annealing (RTA) has been widely used in semiconductor device processing. However, the rise time of RTA, limited to the millisecond (ms) range, is unsuitable for advanced nanometer-scale electronic devices. Using sub-energy bandgap (EG) 532 nm ultra-fast 15 nanosecond (ns) pulsed laser annealing, a record-high dielectric constant (high-κ) of 67.8 and a capacitance density of 75 fF/μm2 at −0.2 V were achieved in Ni/ZrO2/TiN capacitors. According to heat source and diffusion equations, the surface temperature of TiN can reach as high as 870 °C at a laser energy density of 16.2 J/cm2, effectively annealing the ZrO2 material. These record-breaking results are enabled by a novel annealing method—the surface plasma effect generated on the TiN metal. This is because the 2.3 eV (532 nm) pulsed laser energy is significantly lower than the 5.0–5.8 eV energy bandgap (EG) of ZrO2, making it unabsorbable by the ZrO2 dielectric. X-ray diffraction analysis reveals that the large κ value and capacitance density are attributed to the enhanced crystallinity of the cubic-phase ZrO2, which is improved through laser annealing. This advancement is critical for monolithic three-dimensional device integration in the backend of advanced integrated circuits.
Preschool Children’s Eating Habits and Parental Nutritional Status
Authors: Bettocchi Silvia , D’Oria Veronica , De Cosmi Valentina , Scaglioni Silvia , Agostoni Carlo , Paglia Luigi , Paglia Michela , Colombo Sara , Braiotta Francesca , Beretta Matteo , Berti Cristiana
Published: February 2025

Background/Objectives: Poor nutrition in early life represents a relevant public health issue globally. The current study aimed to characterize eating habits among preschoolers and investigate the relationship with parents’ nutritional status. Methods: A secondary analysis of a cross-sectional study carried out at the Child Dentistry Clinics of the Istituto Stomatologico Italiano, Milan, Italy, including 171 patients aged 12–71 months and their parents, was conducted. Demographic data, anthropometric measurements, and information on children’s eating habits were collected. Results: The duration of exclusive breastfeeding was 5.9 (±6.8) months in 65% of children, and complementary feeding began at a mean (±SD) of 6.2 (±1.7) months in accordance with present recommendations. In contrast, the consumption of fruit juice 3.2 (±2.9) times/week, a protein intake of 3.0 (±0.6) g/kg, and a sugar intake of 20 (±8)% were over guideline limits. Overweight/obese children were introduced to cow’s milk earlier (p = 0.033) and consumed a higher percentage of total fats (p = 0.026) whilst consuming a lower percentage of carbohydrates (p = 0.050). In terms of children with both parents being obese or overweight, they consumed more carbohydrates (p = 0.048). Finally, we found that birth weight correlated positively with maternal BMI (ρ = 0.159; p < 0.05). The number of offspring correlated positively with the weekly frequency of sugar-sweetened beverage consumption before 24 months (ρ = 0.282; p < 0.001) whilst correlating negatively with the age of cow’s milk introduction (ρ = −0.226; p < 0.01). Conclusions: Our findings, according to recommendations, suggest that the prevention of obesity needs to begin in infancy. As parents play a pivotal role in establishing children’s food choices, nutritional education aimed at families is needed.
Pathogenicity of Highly Pathogenic Avian Influenza A/H5Nx Viruses in Avian and Murine Models
Authors: Mahmoud Sara H. , Khattab Marwa S. , Yehia Nahed , Zanaty Ali , Arafa Abd El Sattar , Khalil Ahmed A.
Published: February 2025

The evolution and adaptation of highly pathogenic avian influenza (HPAI) viruses pose ongoing challenges for animal and public health. We investigated the pathogenic characteristics of the newly emerged H5N1/2022 and H5N8/2022 of clade 2.3.4.4b compared to the previously circulating H5N1/2016 of clade 2.2.1.2 in Egypt using both avian and murine models. All strains demonstrated a 100% mortality in chickens after intranasal inoculation (106 EID50), while the H5N8/2022 strain showing significantly higher viral shedding (8.34 ± 0.55 log10 EID50). Contact transmission rates varied between strains (50% for the 2.3.4.4b clade and 100% for the 2.2.1.2 clade). In the mouse model, H5N1/2016 infection resulted in an 80% mortality rate with significant weight loss and virus replication in organs. In contrast, H5N8/2022 and H5N1/2022 had 60% and 40% mortality rates, respectively. An histopathological analysis revealed pronounced lesions in the tissues of the infected mice, with the most severe lesions found in the H5N1/2016 group. These findings suggest the decreased pathogenicity of the newer H5Nx strains in mammalian models, emphasizing the need for continued surveillance and adaptive control strategies.
Correction: Shkalim Zemer et al. Pathogens Causing Pediatric Community Acquired Urinary Tract Infections and Their Increasing Antimicrobial Resistance: A Nationwide Study. Pathogens 2024, 13, 201
Authors: Shkalim Zemer Vered , Ashkenazi Shai , Levinsky Yoel , Richenberg Yael , Jacobson Eyal , Nathanson Shay , Shochat Tzippy , Kushnir Shiri , Cohen Moriya , Cohen Avner Herman
Published: February 2025

In the original publication [...]
Varicella Zoster Virus Infection and Pregnancy: An Optimal Management Approach
Authors: Ion Ana , Orzan Olguța Anca , Bălăceanu-Gurău Beatrice
Published: February 2025

Varicella-zoster virus is an α-herpes virus with a double-stranded DNA genome, which causes two main clinical pictures: varicella or chickenpox and herpes zoster. Chickenpox is the primary infection, predominantly affecting children, and it presents with fever and a cutaneous eruption consisting of a vesicular, pruritic, and painful rash. Herpes zoster is a viral infection that typically develops in adulthood as a result of the reactivation of the varicella-zoster virus. If acquired during pregnancy, chickenpox may be responsible for serious complications for the mother, the fetus, or the newborn. The most frequent complication of primary varicella-zoster virus infection in mothers is varicella pneumonia, while encephalitis and hepatitis are rare. The effects on the fetus due to chickenpox infection depend on the stage of pregnancy when the mother becomes infected. If the infection occurs during the first trimester, it does not increase the risk of miscarriage. However, if the infection occurs during the first or second trimester, it may cause fetal varicella syndrome or congenital varicella syndrome. During pregnancy, if the varicella-zoster virus reactivates, it usually does not cause harm to the fetus or lead to any birth defects. However, it may increase maternal morbidity due to herpes zoster and its complications. In the case of primary varicella-zoster virus infection in pregnant women, about 20% of newborns may get neonatal or infantile herpes zoster without any complications. However, it is recommended to start early treatment of herpes zoster in pregnant women as it is believed to accelerate the healing process of skin lesions and alleviate pain, reducing both its duration and severity. Through this narrative review, we discuss the approach to the optimal management of varicella-zoster virus infection during pregnancy.
Potential of Newly Synthesized Sea Buckthorn Phytocarriers as Anti-Inflammatory Active Agents
Authors: Popescu Ionela Daniela , Codrici Elena , Pop Sevinci , Fertig Tudor Emanuel , Dudău Maria , Anghelache Iliuta Laurentiu , Constantin Nicoleta , Marinescu Radu Marian , Voiculescu Vlad Mihai , Badea Georgiana Ileana , Diaconu Mirela , Maxim Monica Elisabeta , Scurtu Mihaela , Zanov Kliment , Enciu Ana-Maria , Litescu Simona Carmen , Tanase Cristiana
Published: February 2025

Background: Phytocarriers are advanced drug delivery systems that use biocompatible and biodegradable materials to enhance the efficacy, stability, and bioavailability of natural products. The sea buckthorn (Hippophae rhamnoides L.) berry extract is rich in essential fatty acids and antioxidants, including vitamin C, vitamin E, and anthocyanins, which contribute to its wide-ranging health benefits. In this study, we assessed the morphology, intracellular delivery, and anti-inflammatory effect of sodium cholate (NaC) and sodium deoxycholate (NaDC)-based phytocarriers loaded with ethanolic extract from sea buckthorn berries (sea buckthorn carrier nanostructures, further defined as phytocarriers). Methods: Negative and electron cryo-microscopy were used to analyze hollow and loaded nanocarriers. The cyto-compatibility of nanocarriers was assessed by endpoint (LDH and MTS) and real-time cell assays, on both human fibroblasts (HS27) and human normal monocytes (SC). The anti-inflammatory effect of hollow and loaded nanocarriers was tested by multiplexing. Results: The negative and electron cryo-microscopy analyses showed that NaC-based phytocarriers were spherical, whilst NaDC-based phytocarriers were predominantly polymorphic. Moreover, the NaDC-based phytocarriers frequently formed large lipid networks or “plaques”. Although 24 h cytotoxicity testing showed both types of nanocarriers are biocompatible with human fibroblasts and monocytes, based on a long-term real-time assay, NaDC delayed fibroblast proliferation. NaC sea buckthorn phytocarriers did not impair fibroblast proliferation in the long term and they were uptaken by cells, as shown by hyperspectral microscopy. NaC nanocarriers and NaC sea buckthorn phytocarriers induced an anti-inflammatory effect, lowering IL-8 cytokine production in normal human monocytes as soon as 4 h of treatment lapsed. Conclusions: NaC-derived phytocarriers loaded with sea buckthorn alcoholic extract are a cell-compatible delivery system with anti-inflammatory properties.
Subcellular Organelle Targeting as a Novel Approach to Combat Tumor Metastasis
Authors: Liu Zefan , Liu Yang , Kang Xin , Li Lian , Xiang Yucheng
Published: February 2025

Tumor metastasis, the spread of cancer cells from the primary site to distant organs, remains a formidable challenge in oncology. Central to this process is the involvement of subcellular organelles, which undergo significant functional and structural changes during metastasis. Targeting these specific organelles offers a promising avenue for enhanced drug delivery and metastasis therapeutic efficacy. This precision increases the potency and reduces potential off-target effects. Moreover, by understanding the role of each organelle in metastasis, treatments can be designed to disrupt the metastatic process at multiple stages, from cell migration to the establishment of secondary tumors. This review delves deeply into tumor metastasis processes and their connection with subcellular organelles. In order to target these organelles, biomembranes, cell-penetrating peptides, localization signal peptides, aptamers, specific small molecules, and various other strategies have been developed. In this review, we will elucidate targeting delivery strategies for each subcellular organelle and look forward to prospects in this domain.
Impact of Co-Spray Drying with Leucine or Trileucine on Aerosol Performance, In Vitro Dissolution, and Cellular Uptake of Colistin Powder Formulations for Inhalation
Authors: Huang Yijing , Arte Kinnari Santosh , Patil Chanakya D. , Zhou Qi , Qu Li
Published: February 2025

Background/Objective: Surface enrichment of hydrophobic excipients via spray drying has been demonstrated as an efficient way to protect the dry powder inhaler formulations against moisture-induced deterioration in aerosol performance. However, the impact of such surface enrichment on dissolution and cellular uptake is less investigated, which can affect the safety and efficacy of dry powder inhalers (DPIs). Methods: In the present work, hygroscopic colistin was coated with leucine or trileucine, at different weight ratios during spray drying. All the powders were exposed to 75% relative humidity for one week. The aerosol performance was compared before and after the moisture exposure. Various solid-state characterizations, including particle size, particle morphology, crystallinity, water sorption/desorption, and surface composition, were conducted to evaluate the properties of spray-dried colistin with/without leucine or trileucine. Results: The results indicated that leucine or trileucine could protect the aerosol performance of spray-dried colistin against moisture deterioration. Leucine crystallized after spray drying with colistin, and such crystal leucine could further hinder water uptake when leucine was at a 20% or higher weight ratio. Trileucine did not crystallize after spray drying with colistin nor reduce the water uptake. Interestingly, trileucine showed a superior moisture protective effect to that of leucine, which could be attributed to its better surface enrichment efficiency than that of leucine due to its lower water solubility. Conclusions: Importantly, our results showed that the surface enrichment with leucine and trileucine did not significantly affect in vitro dissolution of colistin in the Franz cell test and cellular uptake of colistin in the H441 lung epithelium cell model, which could be attributed to small particle size and incomplete surface coverage by leucine or trileucine.
Phytochemical Profiles, Antimicrobial and Antioxidant Activity of Knautia integrifolia (L.) Bertol. subsp. integrifolia
Authors: Kılınc Hilal
Published: February 2025

The genus Knautia (L.) (Caprifoliaceae) is widely distributed in the Mediterranean region and is represented by 11 species of flora in Turkey. This study conducted a detailed phytochemical investigation of the methanol extract of the whole plant of K. integrifolia using a combination of LC-ESI-FT-MS and NMR analyses. According to the results of this analysis, 25 compounds were identified in the methanol extract of K. integrifolia. The extract is particularly rich in phenolic secondary metabolites, including phenolic acid derivatives, flavonoid glycosides, and flavones, along with the presence of triterpenoid compounds. Additionally, the total phenolic content of the K. integrifolia methanol extract was evaluated. Considering the pharmacological activities reported for Knautia species, the antioxidant potential of the methanol extract was assessed using the DPPH radical scavenging assay, resulting in a value of 77.5% when compared to the ascorbic acid standard. In this study, antimicrobial activity tests were performed on K. integrifolia methanol extract for the first time. The results indicated that the extract demonstrated greater susceptibility to Staphylococcus epidermidis compared to the control group. At the same time, Pseudomonas aeruginosa exhibited a minimum inhibitory concentration value, indicating high sensitivity to the methanol extract.
Interpopulational Variation in Cyclotide Production in Heavy-Metal-Treated Pseudometallophyte (Viola tricolor L.)
Authors: Miszczak Rebecca , Slazak Blazej , Sychta Klaudia , Göransson Ulf , Nilsson Anna , Słomka Aneta
Published: February 2025

It remains an open question whether violets use universal mechanisms, such as the production of metallothioneins, phytochelatins, and organic acids and/or rely on specific mechanisms like the production of antimicrobial cyclic peptides (cyclotides) for heavy metal tolerance. To contribute to the understanding of the role of cyclotides, we used seed-derived plants from metallicolous (M) and non-metallicolous (NM) populations of Viola tricolor, a pseudometallophyte tolerant to Zn and Pb. Eight- to ten-week-old plants were treated with 1000 μM of Zn or Pb for 3 or 7 days and subsequently measured for cyclotides and heavy metal content using MALDI-MS and Atomic Absorption Spectrometry (AAS), respectively. Individuals from the M population accumulated a similar amount of Zn but occasionally more Pb in comparison with the NM population. Of the 18 different cyclotides included in the analysis, some showed statistically significant changes under the heavy metal treatment. In general, a decrease was observed in the M population, whereas an increase was observed in the NM population (except for the 3-day treatment with Zn). The day of treatment and dose of metal and their interaction played a crucial role in the explained variance for cyclotides produced by the M individuals but not for the NM plants. This unravels the importance of this antimicrobial compound in heavy metal tolerance and indicates that, in V. tricolor, cyclotides are involved in heavy metal tolerance, but specimens from two populations have developed different strategies and tolerance mechanisms involving cyclotides to mitigate heavy metal stress.
Life Cycle Assessment of Primary Aluminum Production
Authors: Lian Xuan , Gao Hanchen , Shen Leiting , Yu Yilan , Wang Yilin , Peng Zhihong
Published: February 2025

Life cycle assessment (LCA) is used to quantitatively analyze the energy consumption and environmental impact of primary aluminum production in China, the United States, and Europe, as well as global average. The results indicate that electricity and fuel contribute more than 60% of the environmental impact of bauxite mining; steam is the greatest contributor to the environmental impact of alumina production by the Bayer process, with a result exceeding 35%; and electricity contributes >50% of the environmental impact of aluminum electrolysis. The environmental impact of primary aluminum production in China is 1.2 times the global average. The contributions of the three stages of primary aluminum production to the total environmental impact of the process in China are, in descending order, aluminum electrolysis (64.33%), alumina production (33.09%), and bauxite mining (2.58%). If the proportion of thermal power in the electricity source structure is reduced from 60% to 0%, the contribution of electricity to the environmental impact of primary aluminum production will decrease from 38% to 2%, and the total environmental impact will decrease by 73%. Therefore, energy conservation and emissions reduction can be realized through the optimization of the power generation structure, adoption of clean energy production, and improvement of the heat utilization rate in production processes.