bluuebunny/crossref_metadata_2025 huggingface dataset:
An Overwhelming Burden of Psychosocial Stress: Life With Ischemic Heart Disease for Midlife Patients With Low Socioeconomic Status and Lack of Flexible Resources
Authors: Rasmussen Amanda Nikolajew , Petersen Kirsten Schultz , Overgaard Charlotte
Published: October 2021

The largest social inequalities in ischemic heart disease are found in the midlife population. These inequalities are often explained by lifestyle factors, while the role of structural factors and psychosocial stress are generally less acknowledged. In this study, we explore the influence of psychosocial stress on life with ischemic heart disease for midlife patients with low socioeconomic status and lack of flexible resources. In-depth interviews were conducted with 18 participants, and a critical hermeneutic approach was used to analyze and interpret data. We found that the participants were exposed to several external psychosocial stressors related to their family relationships, employment conditions, and experiences of stigmatization. These stressors reinforced each other and created an overwhelming burden of psychosocial stress. Our findings call for supportive interventions that target external psychosocial stressors and stressful feelings among this vulnerable group of patients.
Digital Technologies and Interventions Against Gender-Based Violence in Rural Areas
Authors: Adams Andrea , Lea Suzanne G. , D’Silva Elsa M.
Published: October 2021

This study reports experiences of combining digital technologies and facilitated interventions to address gender-based violence in rural areas. The methodology was based on the Safecity platform with a combination of communicative methods, digital technologies, and participant-led interventions to address gender-based violence in the State of Bihar and the Satara district in rural India. The findings indicate that the most common barriers to creating change in rural communities include patriarchal mindsets that foster a culture of silence around women's rights, lack of education, digital illiteracy, and lack of access to digital tools and services. Notwithstanding these obstacles, rural Indian women and girls participated in an intervention to create a new narrative informed by technological solutions that addressed gender violence in their communities.
Influence of graphene fillers on vibration characteristics of tapered hybrid GFRP composite beams under elevated temperature condition: Numerical and experimental study
Authors: Pawgi Akshay , Krishna Akshay Bharadwaj , Gupta Shikhar , Praveen A Paul , Arumugam Ananda Babu , Sudhagar P Edwin
Published: October 2021

In this study, numerically and experimentally the dynamic characteristics of graphene-reinforced glass fiber–reinforced polymer hybrid uniform and thickness tapered laminated composite beams were investigated. First, the graphene-epoxy nanocomposite solution without and with 0.25, 0.50, and 0.75 wt.% of graphene reinforcement is prepared by the heat shearing technique and then used for the fabrication of glass fiber–reinforced polymer hybrid uniform and thickness tapered composite beams using the hand lay-up method. The elastic properties of the hybrid beams were evaluated using the impulse excitation of vibration technique (ASTM E1876-15) under elevated temperature. Further, the numerical and experimental modal analysis of the hybrid beams with uniform and tapered configurations were conducted with variation in wt.% of graphene particles under fixed-fixed and fixed–free end supports. The results reveal that the natural frequencies of the glass fiber–reinforced polymer hybrid uniform and tapered configurations with 0.25 wt.% of graphene are greater than those of the glass fiber–reinforced polymer beams without graphene reinforcement and observed lesser for 0.5 and 0.75 wt.% of graphene under fixed-fixed and fixed-free end supports, respectively, due to unavoidable agglomeration effects. Furthermore, the parametric study was performed with the influence of weight fraction of graphene and temperature on the transverse response of the tapered composite beam. Hence, it can be concluded that the use of graphene filler in the glass fiber–reinforced polymer composites in the tapered composite beams improves the bending natural frequencies significantly when the weight fraction of the graphene is used lesser as agglomeration is unavoidable in practical condition. Therefore, the comprehensive numerical and experimental work presented in this study will be useful to the designers while using graphene fillers to improve the bending characteristics of the tapered composite beams.
Elevated serum creatine kinase levels due to osimertinib: A case report and review of the literature
Authors: Sugimoto Hiroshi , Matsumoto Satoshi , Tsuji Yukio , Sugimoto Keisuke
Published: October 2021

Introduction

Osimertinib is a tyrosine kinase inhibitor that targets the epidermal growth factor receptor. Elevated serum creatine kinase level is an uncommon adverse event associated with osimertinib treatment for lung cancer.

Case Report

We report a previously healthy 56-year-old woman who developed elevated serum creatine kinase levels during osimertinib monotherapy for epidermal growth factor receptor mutation-positive lung adenocarcinoma.

Management & Outcome

During treatment, she experienced leg cramps and her serum creatine kinase levels increased, peaking at 989 U/l. Further investigation revealed no evidence of cardiotoxicity or myositis; thus, osimertinib-induced myopathy was assumed to be the cause of her elevated serum creatine kinase levels. We successfully managed both lung cancer and osimertinib-induced myopathy using 1-week pauses of osimertinib therapy without dose reduction.

Discussion

Short-term suspension of osimertinib without dose reduction may be a reasonable option for osimertinib-induced myopathy.
Predicting Risk of Reoffending in Persons with Child Sexual Exploitation Material Offense Histories: The Use of Child Pornography Offender Risk Tool in a Scottish Population
Authors: Savoie Valérie , Quayle Ethel , Flynn Elizabeth , O’Rourke Suzanne
Published: October 2021

In the past decade, there has been an increase in child sexual exploitation material (CSEM) offenses and convictions. Although research shows that individuals with CSEM offence histories generally are at low risk of reoffending, certain factors do increase in CSEM convictions, in order to assist with case prioritization, management and supervision, risk assessment is helpful across agencies. The Child Pornography Offender Risk Tool (CPORT) was created specifically for this population and shows significant predictive validity for various outcomes. This study aimed to validate the use of the CPORT in a Scottish sample of 141 adult males who were convicted of CSEM offenses. Receiver Operating Characteristic (ROC) and logistic regression analyses indicated that the CPORT significantly predicted any recidivism (Area Under the Curve = .81), any sexual recidivism (AUC = .78) and CSEM recidivism (AUC = .74), suggesting that it is a valid risk assessment tool for Scottish populations. Recommendations for further research and clinical implications are discussed.
Evaluation of Afterschool Activity Programs’ (ASAP) Effect on Children’s Physical Activity, Physical Health, and Fundamental Movement Skills
Authors: Crozier Mitchell , Wasenius Niko S. , Denize Kathryn M. , da Silva Danilo F. , Nagpal Taniya S. , Adamo Kristi B.
Published: October 2021

Background

Physical literacy-focused afterschool activity programs (ASAPs) can be an effective strategy to improve children’s health-related parameters. We sought to compare physical activity, body composition, aerobic capacity, and fundamental movement skills between physical literacy-focused ASAP and a standard recreational ASAP.

Method

A pre–post (6 months) comparison study was conducted in 5- to 12-year-old children in a physical literacy-focused ASAP (physical literacy group, n = 14) and children attending a standard recreational ASAP (comparison group, n = 15). Physical activity guideline adherence was assessed using accelerometry, body composition was analyzed using bioelectrical impedance, aerobic capacity was estimated using the Progressive Aerobic Cardiovascular Endurance Run test, and fundamental movement skills were evaluated using the Test of Gross Motor Development–2.

Results

There were no significant differences between groups at baseline. After 6 months, the physical literacy group exhibited a significant improvement in their total raw score for the Test of Gross Motor Development–2 ( p = .016), which was likely due to improvements in object control skills ( p = .024). The comparison group significantly increased body mass index ( p = .001) and body fat ( p = .009) over time. No significant between-group differences were found; however, there was a trend for improved aerobic capacity in the physical literacy group ( d = 0.58).

Conclusions

Engagement in the physical literacy-focused ASAP contributed to an attenuated increase in adiposity and an improvement in object control skills.
Negotiating Masculinity in a Post-Socialist Society: The Case of Chinese Male Nurses
Authors: Chan Anita Kit Wa , Fang Tevin Shuhan
Published: October 2021

Occupational gender inequalities are an important sociological concern. Studies on men in female-dominated occupations, such as nursing, have offered rich insights to advance our understanding of gender dynamics in contemporary society. However, current theoretical and empirical accounts have been mostly dominated by Westernized discussions. Utilizing insights from critical studies on men and masculinities, alongside Chinese masculinities studies, this article seeks to enrich the discourse by examining the working experiences of 12 Chinese male nurses in a city hospital. It identifies a culturally specific stigma faced by filial Chinese men. It unravels the masculinity strategies and gender capital, and their effects, used by Chinese male nurses to defend their ability and contributions. It reveals the difficulties and paradoxes arising when men negotiate new hegemonic masculine ideals in post-socialist China. This article provides a nuanced understanding of non-Western masculinities and insights into practices that have been used to sustain male dominance in female-dominated and emotionally demanding jobs.
Pretreatment Squamous Cell Carcinoma Antigen (SCC-Ag) as a Predictive Factor for the Use of Consolidation Chemotherapy in Cervical Cancer Patients After Postoperative Extended-Field Concurrent Chemoradiotherapy
Authors: Zhang Guangyu , Miao Li , Wu Haijian , Zhang Youzhong , Fu Chunli
Published: October 2021

Objective: The goal of this study was to confirm the clinical value of pretreatment serum squamous cell carcinoma antigen (SCC-Ag) in the administration of consolidation chemotherapy in patients with cervical cancers undergoing postoperative extended-field radiotherapy (EFRT) and concurrent chemotherapy (CCRT). Methods: Between 2007 and 2018, a total of 113 patients were treated with postoperative extended-field intensity-modulated radiotherapy (EF-IMRT) and CCRT. There were 63 patients receiving extended-field concurrent chemoradiotherapy (EF-CCRT) and consolidation chemotherapy, while another 50 patients underwent EF-CCRT alone. For the planning target volume, the prescribed dose was 45 to 50.4Gy/25 to 28 fractions. The consolidation chemotherapy regimen contained docetaxel and cisplatin. Results: For the patients with high pretreatment SCC-Ag, the addition of consolidation chemotherapy significantly improved their treatment outcomes and they had better 5-year overall survival (OS) (81.02% vs 63.44%, P = .018) and disease-free survival (DFS) (76.95% vs 61.12%, P = .007) than those without it. Meanwhile, the patients with consolidation chemotherapy had a lower rate of distant metastasis (8.8% vs 34.8%, P = .001). For the patients with low pretreatment SCC-Ag, there was no difference in prognosis between patients receiving consolidation chemotherapy and those not receiving consolidation. In multivariate analysis, consolidation chemotherapy was found to be a factor significantly associated with DFS ( P = .035, 95% confidence interval (CI): 0.304-0.977) and distant metastasis ( P = .009, 95% CI: 0.125-0.841). Conclusion: The patients who received consolidation chemotherapy showed significantly better DFS. Furthermore, pretreatment serum SCC-Ag > 6.5 ng/mL may be a predictive factor for the use of consolidation chemotherapy in cervical cancer patients treated with postoperative EF-CCRT.
Usefulness of ultrasound fusion technology for hepatocellular carcinoma localisation, pre- and post-thermal ablation
Authors: Afif A Mohamed , Laroco OD , Lau SMD , Teo SM , Rahman AS Abdul , Too CW , Venkatanarasimha N , Gogna A
Published: October 2021

Introduction

Percutaneous thermal ablation of inconspicuous lesions can be challenging. Fusion ultrasound (FUS) allows the use of previously performed diagnostic imaging like computed tomography (CT), magnetic resonance imaging (MRI) and positron emission tomography (PET–CT) to localise hepatocellular carcinoma (HCC) for treatment. This paper illustrates FUS case studies of pre-, intra- and post-ablation imaging of inconspicuous HCC, supplemented by use of contrast-enhanced ultrasound (CEUS).

Method

Four prospective cases during September 2014 to October 2018, with HCC amenable to ablation, which were poorly identified on ultrasound, underwent FUS. FUS pre-screening was scheduled within three months of the previous CT or MRI, and between one to four weeks prior to the scheduled ablation date. Post-ablation imaging with FUS was performed between four to six weeks to coincide with their routine follow-up CT or MRI.

Findings

There were potential benefits observed in the cases with combined techniques of FUS and CEUS for limiting circumstances such as heat sink effect, multiple lesions targeting, inconspicuous lesion detection and pre-ablation technical feasibility assessment.

Discussion

The combined use of FUS and CEUS improves tumour visibility, increases operator imaging confidence and reduces heat sink effect during percutaneous thermal ablation.

Conclusion

FUS imaging is helpful in targeting poor conspicuity lesions that cannot be detected on grey-scale ultrasound. It facilitates in ensuring optimal treatment of hepatic lesions for improvement of patient prognosis and follow-up imaging.
Carrots, not Sticks: A Historical Analysis of Beijing's Economic Statecraft Towards Hong Kong
Authors: Summers Tim
Published: October 2021

This analysis offers a historical assessment of “economic statecraft” in Beijing's approach to Hong Kong from 1997 to 2020. It discusses how the concept of “economic statecraft” can be applied to Beijing–Hong Kong relations given the nature of the “one country, two systems” framework, and looks at some differing perceptions about economic statecraft in Hong Kong. It argues that, during this period, economic tools were in general used by Beijing relatively sparingly, and in the form of inducements rather than coercion. In conclusion, the analysis suggests that the contested interpretations of Beijing–Hong Kong economic relations demonstrate that “economic statecraft” is to a certain extent in the eye of the beholder.
School nurses’ experiences and challenges of working with childhood obesity in Northern Sweden: A qualitative descriptive study
Authors: Gothilander Jennifer , Johansson Helene
Published: October 2021

Childhood obesity is increasing in Sweden. All children are offered regularly spread health visits to a school nurse. As health visits include a measure of height and weight and a health dialogue, school nurses can discover, disclose, and treat a child's weight gain. The aim of this study was to describe school nurses’ experiences and challenges in working with childhood obesity. This qualitative study collected data through focus-group discussion and semi-structured interviews with ten female school nurses from six municipalities. Data were analysed inductively using manifest qualitative content analysis. The study was reported using the COREQ guidelines. Stigmatization and lack of resources are major challenges for school nurses working with childhood obesity, and they experience frustration, powerlessness and feel that they provide unequal treatment. The present study concludes that obesity stigmatization is a widespread challenge for school nurses. They cannot alone generate all the resources needed or conquer all challenges. Evidence-based guidelines, increased knowledge, time for reflections and peer support could potentially empower school nurses, reduce frustration, and improve the quality of and equality in childhood obesity treatment.
Why the NLD fails to consolidate democratic transition in Myanmar
Authors: Swe Zayar Lay
Published: October 2021

The general populace in Myanmar, as well as international observers, have expected that the National League for Democracy (NLD) would be able to consolidate democratic transition, since the latter obtained a certain degree of support from home and abroad. During the five years of the NLD administration, transition has nevertheless been in regression instead of progression. All rating agencies (Freedom House, Bertelsmann, VDem) consider that Myanmar is not yet a democracy. Freedom House's report indicates that Myanmar's status changed from ‘Partly Free’ to ‘Not Free’ in 2020. Much of the existing literature argues that this stems from the NLD having had to operate within structural constraints and agency curbs. Therefore, this article will examine why the NLD is unable to overcome these barriers, under which structural factors and what kind of agency. The article argues that, instead of structure-altering, the NLD has reinforced structural barriers without seeking any considerable leeway within the bounds of existing constraints. In the context of structure, this article will examine the transition process itself, as well as constitutional arrangements, the economic system, and the political culture. The agency context will include the behaviours of the chief executive, the Tatmadaw, and the general populace.
Functional and Structural Outcomes After Retears of Arthroscopically Repaired Large and Massive Rotator Cuff Tears
Authors: Tanaka Suguru , Gotoh Masafumi , Tanaka Koji , Mitsui Yasuhiro , Nakamura Hidehiro , Ozono Hiroki , Okawa Takahiro , Shiba Naoto
Published: October 2021

Background:

Most studies have shown acceptable clinical results in patients with large or massive tears treated by arthroscopic rotator cuff repair (ARCR); however, the effects of retears after surgery in these patients remain unknown.

Purpose:

To evaluate functional and structural outcomes after retears of large and massive rotator cuff tears treated by ARCR.

Study Design:

Case series; Level of evidence, 4.

Methods:

A total of 196 consecutive patients with large to massive rotator cuff tears underwent physical examination and magnetic resonance imaging before and after ARCR at 6, 12, and 24 months. Of these, 9 patients were lost at 6 months after surgery. Therefore, 187 patients were followed up for 24 months after surgery; 148 patients showed no postsurgical ruptures. Consequently, the remaining 39 patients with postsurgical ruptures were included in this study (mean age at surgery, 64.2 ± 8.7 years). Functional outcome measures comprised the University of California, Los Angeles (UCLA) and Japanese Orthopaedic Association (JOA) scores. Structural outcome measures consisted of the global fatty degeneration index (GFDI), mediolateral tear size, and residual tendon attachment area as evaluated by our own scoring system.

Results:

The mean UCLA and JOA scores significantly improved from 16.3 ± 3.9 and 63.2 ± 10.7 preoperatively to 27.9 ± 5.5 ( P < .0001) and 84.5 ± 9.4 ( P < .0001) at final follow-up, respectively. The mean mediolateral tear size ( P = .03, .02, and .02, respectively) and residual tendon attachment area ( P = .04, .03, and .04, respectively) significantly improved from preoperatively to 6, 12, and 24 months postoperatively. The correlation analysis between the functional and structural variables confirmed significant associations between the residual tendon attachment area, the JOA and UCLA scores at 24 months postoperatively, and the preoperative GFDI ( r = –0.81 to 0.78).

Conclusion:

The residual tendon attachment area after a retear was significantly larger at 24 months after surgery than before surgery. In addition, significant associations were confirmed between preoperative fatty degeneration, the residual tendon attachment area, and functional outcomes after a retear. These results may explain why functional outcomes significantly improved even after retears in this series.
Resources in Relational Packages: Social Capital as a Byproduct of Relational Work
Authors: Stoltz Dustin S. , Pitluck Aaron Z.
Published: October 2021

Social capital theory offers a compelling explanation as to why people are committed to making resources available to others outside of formal institutions. In this article, we build on social capital theory to explain how actors overcome two practical problems endemic to these resource transfers. We present Viviana Zelizer’s relational work theory as a complimentary framework which accounts for when an individual may act on commitments to offer resources and which commitments to act upon when they are in conflict. Drawing on our empirical work on almsgiving to social outcasts and resource transfers at mourning ceremonies in Azerbaijan, we describe how people identify and ascribe their relationships to others by relying on available cultural conventions to mark economic transactions and other media as appropriate or inappropriate. By conceptualizing social capital in this way, we also obtain a process-tracing methodology useful for social researchers and for community activists to generate ideas on how to expand social capital in their own or others’ communities.
Factors Affecting the Satisfaction Levels of Patients who Underwent Orthopedic Knee Surgery in the Early Postoperative Period
Authors: Erden Arzu , Emirzeoğlu Murat
Published: October 2021

The aim of this study was to investigate the effects of biopsychosocial parameters in the early postoperative period on the satisfaction of patients. Seventy-seven patients (female/male: 61/16, age: 40.28 ± 17.67 years) who underwent arthroscopic meniscectomy (n = 43), anterior cruciate ligament reconstruction (n = 25) and total knee arthroplasty (n = 9) were included in this study. The satisfaction levelwas evaluated using the Orthopedics Service Inpatient Satisfaction Survey (OTISS). In addition, the pain intensity, anxiety, depression, and independence level in daily living activities of the patients were evaluated. There was a very weak negative correlation between the anxiety and the satisfaction level with the physiotherapist and secretary ( r: −0.274, p: 0.016; r:−0.265, p: 0.020). A very weak negative correlation was found between the pain intensity at activity and satisfaction with the nurse ( r: −0.227, p: 0.048). There were very weak correlations between feeding and satisfaction with the physiotherapist ( r: 0.292, p: 0.010), secretary ( r: 0.285, p: 0.012), doctor ( r: −0.269, p: 0.018), nurse ( r: 0.300, p: 0.008) general satisfaction ( r: 0.251, p: 0.028) and OTISS total score ( r: −0.305, p: 0.007). In conclusion, the pain intensity, anxiety, and independence level in the early postoperative period were related to the satisfaction levels of patients who have undergone orthopedic knee surgery.
Psychological predictors of negative treatment outcome with Erenumab in chronic migraine: data from an open label long-term prospective study
Authors: Bottiroli Sara , De Icco Roberto , Vaghi Gloria , Pazzi Stefania , Guaschino Elena , Allena Marta , Ghiotto Natascia , Martinelli Daniele , Tassorelli Cristina , Sances Grazia
Published: October 2021

Background

Monoclonal antibodies (mABs) targeting the calcitonin gene-related peptide (CGRP) pathway represent the first disease-specific preventive migraine therapy. Growing evidence suggests that they are effective in the preventive treatment of difficult-to-treat patients. In this study, we evaluated the psychological predictors of the outcome of treatment with the anti-CGRP monoclonal antibody erenumab in patients with chronic migraine (CM).

Methods

Seventy-five patients with CM who had already failed at least 3 preventive therapies received erenumab every 28 days for a period of 12 months. Before the first administration, patients received a full psychological evaluation using The Structured Clinical Interview for DSM-5 Clinician Version (SCID-5-CV) to assess personality disturbances (primary outcome), mood and anxiety disorders, and as well specific questionnaires to evaluate alexithymia traits, childhood traumas, and current stressors (secondary outcomes).

Results

After 12 months of treatment, 53 patients reported a reduction of at least 50% in headache days/per month (Responders), whereas 22 did not (Non Responders). When compared to Responders, Non Responders were characterized by a higher prevalence of personality disorders belonging to Cluster C (avoidant, dependent, and obsessive-compulsive) (77% vs 37%,p = .001). Non Responders were also characterized by a higher prevalence of anxiety disorders (90% vs 60%,p = 0.007), showed more alexithymic traits (51.7 ± 13.7 vs 42.9 ± 14.3,p = 0.017), and reported a higher number of 'at least serious' current stressors (3.2 ± 4.0 vs 0.8 ± 1.4,p < .0001) than Responders. At the multivariate analysis, higher prevalence of Cluster C personality disorders (OR 3.697;p = 0.05) and higher number of ‘at least serious’ life events (OR 1.382;p = 0.017) arose as prognostic factors of erenumab failure.

Conclusions

Erenumab confirmed its effectiveness in a population of difficult-to-treat migraine. The presence of “anxious-fearful” personality together with current stressors and anxiety represent negative predictors of treatment outcome.

Trial registration

The study protocol was registered at clinicaltrials.gov (NCT04361721).
SamQL: a structured query language and filtering tool for the SAM/BAM file format
Authors: Lee Christopher T. , Maragkakis Manolis
Published: October 2021

Background

The Sequence Alignment/Map Format Specification (SAM) is one of the most widely adopted file formats in bioinformatics and many researchers use it daily. Several tools, including most high-throughput sequencing read aligners, use it as their primary output and many more tools have been developed to process it. However, despite its flexibility, SAM encoded files can often be difficult to query and understand even for experienced bioinformaticians. As genomic data are rapidly growing, structured, and efficient queries on data that are encoded in SAM/BAM files are becoming increasingly important. Existing tools are very limited in their query capabilities or are not efficient. Critically, new tools that address these shortcomings, should not be able to support existing large datasets but should also do so without requiring massive data transformations and file infrastructure reorganizations.

Results

Here we introduce SamQL, an SQL-like query language for the SAM format with intuitive syntax that supports complex and efficient queries on top of SAM/BAM files and that can replace commonly used Bash one-liners employed by many bioinformaticians. SamQL has high expressive power with no upper limit on query size and when parallelized, outperforms other substantially less expressive software.

Conclusions

SamQL is a complete query language that we envision as a step to a structured database engine for genomics. SamQL is written in Go, and is freely available as standalone program and as an open-source library under an MIT license, https://github.com/maragkakislab/samql/.
shinyCurves, a shiny web application to analyse multisource qPCR amplification data: a COVID-19 case study
Authors: Olaechea-Lázaro S. , García-Santisteban I. , Pineda J. R. , Badiola I. , Alonso S. , Bilbao Jose Ramon , Fernandez-Jimenez Nora
Published: October 2021

Background

Quantitative, reverse transcription PCR (qRT-PCR) is currently the gold-standard for SARS-CoV-2 detection and it is also used for detection of other virus. Manual data analysis of a small number of qRT-PCR plates per day is a relatively simple task, but automated, integrative strategies are needed if a laboratory is dealing with hundreds of plates per day, as is being the case in the COVID-19 pandemic.

Results

Here we present shinyCurves, an online shiny-based, free software to analyze qRT-PCR amplification data from multi-plate and multi-platform formats. Our shiny application does not require any programming experience and is able to call samples Positive, Negative or Undetermined for viral infection according to a number of user-defined settings, apart from providing a complete set of melting and amplification curve plots for the visual inspection of results.

Conclusions

shinyCurves is a flexible, integrative and user-friendly software that speeds-up the analysis of massive qRT-PCR data from different sources, with the possibility of automatically producing and evaluating melting and amplification curve plots.
Transcriptome profile of the sinoatrial ring reveals conserved and novel genetic programs of the zebrafish pacemaker
Authors: Minhas Rashid , Loeffler-Wirth Henry , Siddiqui Yusra H. , Obrębski Tomasz , Vashisht Shikha , Abu Nahia Karim , Paterek Alexandra , Brzozowska Angelika , Bugajski Lukasz , Piwocka Katarzyna , Korzh Vladimir , Binder Hans , Winata Cecilia Lanny
Published: October 2021

Background

Sinoatrial Node (SAN) is part of the cardiac conduction system, which controls the rhythmic contraction of the vertebrate heart. The SAN consists of a specialized pacemaker cell population that has the potential to generate electrical impulses. Although the SAN pacemaker has been extensively studied in mammalian and teleost models, including the zebrafish, their molecular nature remains inadequately comprehended.

Results

To characterize the molecular profile of the zebrafish sinoatrial ring (SAR) and elucidate the mechanism of pacemaker function, we utilized the transgenic line sqet33mi59BEt to isolate cells of the SAR of developing zebrafish embryos and profiled their transcriptome. Our analyses identified novel candidate genes and well-known conserved signaling pathways involved in pacemaker development. We show that, compared to the rest of the heart, the zebrafish SAR overexpresses several mammalian SAN pacemaker signature genes, which include hcn4 as well as those encoding calcium- and potassium-gated channels. Moreover, genes encoding components of the BMP and Wnt signaling pathways, as well as members of the Tbx family, which have previously been implicated in pacemaker development, were also overexpressed in the SAR. Among SAR-overexpressed genes, 24 had human homologues implicated in 104 different ClinVar phenotype entries related to various forms of congenital heart diseases, which suggest the relevance of our transcriptomics resource to studying human heart conditions. Finally, functional analyses of three SAR-overexpressed genes, pard6a, prom2, and atp1a1a.2, uncovered their novel role in heart development and physiology.

Conclusion

Our results established conserved aspects between zebrafish and mammalian pacemaker function and revealed novel factors implicated in maintaining cardiac rhythm. The transcriptome data generated in this study represents a unique and valuable resource for the study of pacemaker function and associated heart diseases.
Genome-wide association mapping uncovers sex-associated copy number variation markers and female hemizygous regions on the W chromosome in Salix viminalis
Authors: Hallingbäck Henrik R. , Pucholt Pascal , Ingvarsson Pär K. , Rönnberg-Wästljung Ann Christin , Berlin Sofia
Published: October 2021

Background

Sex chromosomes are in some species largely undifferentiated (homomorphic) with restricted sex determination regions. Homomorphic but different sex chromosomes are found in the closely related genera Populus and Salix indicating flexible sex determination systems, ideal for studies of processes involved in sex chromosome evolution. We have performed genome-wide association studies of sex and analysed sex chromosomes in a population of 265 wild collected Salix viminalis accessions and studied the sex determining locus.

Results

A total of 19,592 markers were used in association analyses using both Fisher’s exact tests and a single-marker mixed linear model, which resulted in 48 and 41 sex-associated (SA) markers respectively. Across all 48 SA markers, females were much more often heterozygous than males, which is expected if females were the heterogametic sex. The majority of the SA markers were, based on positions in the S. purpurea genome, located on chromosome 15, previously demonstrated to be the sex chromosome. Interestingly, when mapping the genotyping-by-sequencing sequence tag harbouring the two SA markers with the highest significance to the S. viminalis genomic scaffolds, five regions of very high similarity were found: three on a scaffold that represents a part of chromosome 15, one on a scaffold that represents a part of chromosome 9 and one on a scaffold not anchored to the genome. Based on segregation differences of the alleles at the two marker positions and on differences in PCR amplification between females and males we conclude that females had multiple copies of this DNA fragment (chromosome 9 and 15), whereas males only had one (chromosome 9). We therefore postulate that the female specific sequences have been copied from chromosome 9 and inserted on chromosome 15, subsequently developing into a hemizygous W chromosome linked region.

Conclusions

Our results support that sex determination in S. viminalis is controlled by one locus on chromosome 15. The segregation patterns observed at the SA markers furthermore confirm that S. viminalis females are the heterogametic sex. We also identified a translocation from chromosome 9 to the W chromosome.
Structural heart defects associated with ETB mutation, a cause of Hirschsprung disease
Authors: Chen Ko-Chin , Chen Ko-Chien , Song Zan-Min , Croaker Geoffrey D.
Published: October 2021

Background

HSCR, a colonic neurocristopathy affecting 1/5000 births, is suggested to associate with cardiac septal defects and conotruncal malformations. However, we question subtle cardiac changes maybe more commonly present due to multi-regulations by HSCR candidate genes, in this instance, ETB. To investigate, we compared the cardiac morphology and quantitative measurements ofsl/slrat to those of the control group.

Methods

Eleven neonatal rats were generated from heterozygote (ETB+/−) crossbreeding. Age and bodyweight were recorded at time of sacrifice. Diffusion-staining protocols with 1.5% iodine solution was completed prior to micro-CT scanning. All rats were scanned using an in vivo micro-CT scanner, Caliper Quantum FX, followed by two quality-control scans using a custom-built ex vivo micro-CT system. All scans were reviewed for gross cardiac dysmorphology. Micro-CT data were segmented semi-automatically post-NLM filtering for: whole-heart, LV, RV, LA, RA, and aortic arch. Measurements were taken with Drishti. Following image analysis, PCR genotyping of rats was performed: fivesl/slrats, three wildtype, and three heterozygotes. Statistical comparisons on organ volume, growth rate, and organ volume/bodyweight ratios were made betweensl/sland the control group.

Results

Cardiac morphology and constituents were preserved. However, significant volumetric reductions were recorded insl/slrats with respect to the control: whole heart (38.70%,p value = 0.02); LV (41.22%,p value = 0.01), RV (46.15%,p value = 0.02), LA (44.93%,p value = 0.06), and RA (39.49%,p value = 0.02). Consistent trend was observed in growth rate (~ 20%) and organ-volume/bodyweight ratios (~ 25%). On the contrary, measurements on aortic arch demonstrated no significant difference among the two groups.

Conclusion

Despite the presence of normal morphology, significant cardiac growth retardation was detected insl/slrat, supporting the likely association of cardiac anomalies with HSCR, at least in ETB−/−subtype. Structural reduction was likely due to a combination of failure to thrive from enteric dysfunction, alterations to CaNCC colonization, and importantly coronary hypoperfusion from elevated ET-1/ETA-mediated hypervasoconstriction. Little correlation was detected between aortic arch development andsl/slrat, supporting minor ETBrole in large vessels. Although further clinical study is warranted, HSCR patients may likely require cardiac assessment in view of potential congenital cardiac defects.
Building the capacity of community health workers to support health and social care for dependent older people in Latin America: a pilot study in Fortaleza, Brazil
Authors: Neto João Bastos Freire , de Moraes Gerídice Lorna Andrade , de Souza Aredes Janaína , Giacomin Karla Cristina , de Melo Luciane Ponte , Sempe Lucas , Lloyd-Sherlock Peter
Published: October 2021

Background

Brazil is seeing rapid population ageing, which is leading to new demands on primary health care services. There is a need to develop and assess the effectiveness of new interventions to build the capacity of staff, including community health workers, to meet the needs of groups such as care-dependent older people and their care-givers. This study examines the feasibility of a small training intervention piloted in the Brazilian city of Fortaleza.

Methods

The study evaluated participants’ own assessments of key knowledge and skills related to the needs of care-dependent older people, both before and after the training intervention. It also assessed their capacity to implement a simple screening tool of geriatric risk factors.

Results

The participant self-assessments indicate significant improvements in their perceived knowledge and capacity in responding to the health needs of care-dependent older people. Additionally, participants were able to successfully conduct the home visits and screening for risk factors.

Conclusions

The study demonstrates the feasibility of developing interventions to enhance the capacity of community health workers to meet the needs of dependent older people in countries like Brazil. The evidence of effectiveness, though limited and subjective, provides justification for a larger, formally evaluated intervention. The experience of Fortaleza provides valuable lessons for other cities and countries in the region which are facing similar challenges.
Factors shaping the COVID-19 epidemic curve: a multi-country analysis
Authors: Jang Su Yeon , Hussain-Alkhateeb Laith , Rivera Ramirez Tatiana , Al-Aghbari Ahmed Asa’ad , Chackalackal Dhia Joseph , Cardenas-Sanchez Rocio , Carrillo Maria Angelica , Oh In-Hwan , Alfonso-Sierra Eduardo Andrés , Oechsner Pia , Kibiwott Kirui Brian , Anto Martin , Diaz-Monsalve Sonia , Kroeger Axel
Published: October 2021

Background

Lockdown measures are the backbone of containment measures for the COVID-19 pandemic both in high-income countries (HICs) and low- and middle-income countries (LMICs). However, in view of the inevitably-occurring second and third global covid-19 wave, assessing the success and impact of containment measures on the epidemic curve of COVID-19 and people’s compliance with such measures is crucial for more effective policies. To determine the containment measures influencing the COVID-19 epidemic curve in nine targeted countries across high-, middle-, and low-income nations.

Methods

Four HICs (Germany, Sweden, Italy, and South Korea) and five LMICs (Mexico, Colombia, India, Nigeria, and Nepal) were selected to assess the association using interrupted time series analysis of daily case numbers and deaths of COVID-19 considering the following factors: The “stringency index (SI)” indicating how tight the containment measures were implemented in each country; and the level of compliance with the prescribed measures using human mobility data. Additionally, a scoping review was conducted to contextualize the findings.

Results

Most countries implemented quite rigorous lockdown measures, particularly the LMICs (India, Nepal, and Colombia) following the model of HICs (Germany and Italy). Exceptions were Sweden and South Korea, which opted for different strategies. The compliance with the restrictions—measured as mobility related to home office, restraining from leisure activities, non-use of local transport and others—was generally good, except in Sweden and South Korea where the restrictions were limited. The endemic curves and time-series analysis showed that the containment measures were successful in HICs but not in LMICs.

Conclusion

The imposed lockdown measures are alarming, particularly in resource-constrained settings where such measures are independent of the population segment, which drives the virus transmission. Methods for examining people’s movements or hardships that are caused by covid- no work, no food situation are inequitable. Novel and context-adapted approach of dealing with the COVID-19 crisis are therefore crucial.
Viral suppression among HIV-positive patients on antiretroviral therapy in northwestern Nigeria: an eleven-year review of tertiary care centre records, January 2009–December 2019
Authors: Abdullahi Suleiman Bello , Ibrahim Olayinka Rasheed , Okeji Abdulkadir Baba , Yandoma Rabilu Iliyasu , Bashir Ibrahim , Haladu Suleiman , Idris Suleiman Ahmad , Oseni T. I. A. , Suleiman Bello Muhammad , Yahaya Mohammed , Aworh Mabel Kamweli , Sufiyan Mu’awiyyah Babale
Published: October 2021

Background

Human Immuno-Deficiency Virus (HIV) remains one of the world’s significant public health challenges. Viral suppression is the key indicator for treatment success in People living with HIV (PLHIV). We determined the level of viral suppression, and its associated factors among PLHIV attending Federal Medical Centre Katsina (FMC Katsina), Nigeria.

Methods

This retrospective descriptive cross-sectional study was conducted on 913 HIV positive adults enrolled in care between January 2009 and December 2019. Information on socio-demographics, clinical, immunological, Viral load (VL), and other relevant parameters were extracted from the patients’ care records. The primary outcome was the proportion of patients that achieved viral suppression. We also analyzed variables that were associated with VL suppression.

Results

Of 913, records of 831 (91.0%) registered patients were analyzed. During the period, 751 (90.4%) achieved viral suppression, 427 (51.4%) had CD4 counts  ≥ 500 and 477 (57.4%) were on HAART for ≥ 5 years. Majority, 793 (95.4%) were on first-line HAART regimen (Tenofovir-Lamivudine-Dolutegravir or Abacavir-Lamivudine-Dolutegravir), and 809 (97.4%) in the non-advanced stage (WHO stages 1 and 2). The median (interquartile range) of viral load was 20 (20–40) vs 19,989 (3311–110,340) cp/ml in virally suppressed, and unsuppressed  respectively. Factors associated with viral suppression included being unemployed (Adjusted OR [AOR] 4.9, 95% CI 2.771, 8.539), educated (AOR 4.2, 95% CI 1.098, 16.223), having a baseline CD4 count ≥ 500 cells/µl (AOR 2.7, 95% CI 1.588, 4.625), and being on first line HAART regimen [AOR 7.0, 95% CI 3.220, 15.648].

Conclusions

Our study demonstrated a good viral suppression among PLHIV on HAART. Variables associated with viral suppression included unemployment, formal education, high baseline CD4 count, and first line HAART regimen.
MRI characteristics of brain edema in preeclampsia/eclampsia patients with posterior reversible encephalopathy syndrome
Authors: Mai Hui , Liang Zhiyu , Chen Zhanhang , Liu Zhaoran , Xu Yaxi , Chen Xuting , Du Xiujian , Peng Yuling , Chen Yonglu , Dong Tianfa
Published: October 2021

Background

The neuroimaging manifestations of eclampsia and preeclampsia often overlap, mainly presenting as posterior reversible encephalopathy syndrome (PRES). The purpose of this retrospective study was to compare the extent and nature of brain edema in eclampsia and preeclampsia patients with PRES based on MRI characteristics.

Methods

One hundred fifty women diagnosed with preeclampsia-eclampsia and undergoing cranial MRI were enrolled; 24 of these were diagnosed as having eclampsia. According to clinicoradiologic diagnosis of PRES, eligible patients were classified as having eclampsia with PRES (group E-PRES) and preeclampsia with PRES (group P-PRES). A scale on T2W FLAIR-SPIR images was established to evaluate the extent of brain edema, and the score of brain edema (SBE) of both groups was compared. In patients of the two groups who also underwent DWI sequence, the presence or absence of hyperintensity on DWI and hypointensity on ADC maps were determined to compare the nature of brain edema. Furthermore, clinical and biochemical data of the two groups were compared.

Results

The incidence of PRES in eclampsia patients was significantly higher than that in preeclampsia patients (87.50% vs. 46.03%, P<0.001). The SBE of all regions and typical regions in group E-PRES patients were significantly higher than those in group P-PRES patients (15.88±8.72 vs. 10.90±10.21, P=0.021; 8.52±3.87 vs. 5.01±4.19, P=0.002; respectively). The presence of hyperintensity on DWI was determined more frequently in group E-PRES patients than group P-PRES patients (71.43% vs. 32.00%, P=0.024). Age, systolic blood pressure, white blood cell count, neutrophil count and percentage of neutrophils were significantly different between the two groups (P<0.05).

Conclusions

Certain MRI characteristics that reflect the extent and nature of brain edema were different between eclampsia and preeclampsia patients with PRES. Additional prospective studies are still required to explore whether these MRI characteristics of brain edema may further become a potential predictor for eclamptic seizures in preeclampsia patients with PRES.
Effect of EPA on Hsp90 and GRα protein expression in multiple myeloma drug-resistant cells
Authors: Wu Shenghao , Chen Yuemiao , Wang Xueshuang , Weng Shanshan , Zhou Wenjin , Liu Zhen
Published: October 2021

Background

Approximately 20% of MM patients harbor glucocorticoid (GC) resistance and are not responsive to therapeutic effect. Chaperoneheat-shock proteins Hsp90 is needed for ligand docking, The imbalance of Hsp90/GRα (glucocorticoid receptor α) may be an important cause of GC resistance. Recent studies have indicated that EPA could repress cancer cell growth by regulating critical influential factors in progression of cancer, consisting of resistance to drugs, chemosensitivity. The aim of the present study was to test the cytotoxic effects of EPA alone or EPA + Dexamethasone in dexamethasone-resistant MM cell (MM.1R) and investigate whether DHA can induce apoptosis and reverse acquired glucocorticoid resistance in dexamethasone-resistant MM cell (MM.1R).

Methods

Cell Counting Kit-8 (CCK-8) was used to detect the proliferation of MM.1R cells after treating with EPA alone and EPA combined with DEX. Mitochondrial membrane potential was measured by flow cytometry and GRα and Hsp90 protein expression were assessed by western blot analysis.

Results

EPA alone was able to inhibit cell proliferation as evidenced by CCK-8 assay and the tumor growth was remarkably suppressed by EPA + Dexamethasone, Cell apoptosis after EPA treatment was obviously observed by Flow cytometry analysis of the mitochondrial membrane potential. Analysis of Hsp90 and GRα proteins in MM.1R cells incubated with EPA revealed down-regulation of Hsp90 and up-regulation of GRα. Accordingly, the Hsp90/GRα ratio was significantly decreased with the increase of EPA concentration.

Conclusions

EPA might be used as a new effective treatment for reversal of glucocorticoid-resistance in multiple myeloma.