bluuebunny/crossref_metadata_2025 huggingface dataset:
Lithium and Erectile Dysfunction: An Overview
Authors: Sheibani Mohammad , Ghasemi Mehdi , Dehpour Ahmad Reza
Published: January 2022

Lithium has been a mainstay of therapy for patients with bipolar disorders for several decades. However, it may exert a variety of adverse effects that can affect patients’ compliance. Sexual and erectile dysfunction has been reported in several studies by patients who take lithium as monotherapy or combined with other psychotherapeutic agents. The exact mechanisms underlying such side effects of lithium are not completely understood. It seems that both central and peripheral mechanisms are involved in the lithium-related sexual dysfunction. Here, we had an overview of the epidemiology of lithium-related sexual and erectile dysfunction in previous clinical studies as well as possible pathologic pathways that could be involved in this adverse effect of lithium based on the previous preclinical studies. Understanding such mechanisms could potentially open a new avenue for therapies that can overcome lithium-related sexual dysfunction and improve patients’ adherence to the medication intake.
Sympathetic Stimulation Upregulates the Ca2+ Channel Subunit, CaVα2δ1, via the β1 and ERK 1/2 Pathway in Neonatal Ventricular Cardiomyocytes
Authors: Al Katat Aya , Zhao Juan , Calderone Angelino , Parent Lucie
Published: January 2022

Intracellular Ca2+ overload secondary to chronic hemodynamic stimuli promotes the recruitment of Ca2+-dependent signaling implicated in cardiomyocyte hypertrophy. The present study tested the hypothesis that sympathetic-mediated hypertrophy of neonatal rat ventricular cardiomyocytes (NRVMs) translated to an increase in calcium influx secondary to the upregulation of CaV1.2 channel subunits. Confocal imaging of norepinephrine (NE)-treated NRVMs revealed a hypertrophic response compared to untreated NRVMs. L-type CaV1.2 peak current density was increased 4-fold following a 24-h stimulation with NE. NE-treated NRVMs exhibited a significant upregulation of CaVα2δ1 and CaVβ3 protein levels without significant changes of CaVα1C and CaVβ2 protein levels. Pre-treatment with the β1-blocker metoprolol failed to inhibit hypertrophy or CaVβ3 upregulation whereas CaVα2δ1 protein levels were significantly reduced. NE promoted the phosphorylation of ERK 1/2, and the response was attenuated by the β1-blocker. U0126 pre-treatment suppressed NE-induced ERK1/2 phosphorylation but failed to attenuate hypertrophy. U0126 inhibition of ERK1/2 phosphorylation prevented NE-mediated upregulation of CaVα2δ1, whereas CaVβ3 protein levels remained elevated. Thus, β1-adrenergic receptor-mediated recruitment of the ERK1/2 plays a seminal role in the upregulation of CaVα2δ1 in NRVMs independent of the concomitant hypertrophic response. However, the upregulation of CaVβ3 protein levels may be directly dependent on the hypertrophic response of NRVMs.
Risk Alleles for Multiple Myeloma Susceptibility in ADME Genes
Authors: Scionti Francesca , Agapito Giuseppe , Caracciolo Daniele , Riillo Caterina , Grillone Katia , Cannataro Mario , Di Martino Maria Teresa , Tagliaferri Pierosandro , Tassone Pierfrancesco , Arbitrio Mariamena
Published: January 2022

The cause of multiple myeloma (MM) remains largely unknown. Several pieces of evidence support the involvement of genetic and multiple environmental factors (i.e., chemical agents) in MM onset. The inter-individual variability in the bioactivation, detoxification, and clearance of chemical carcinogens such as asbestos, benzene, and pesticides might increase the MM risk. This inter-individual variability can be explained by the presence of polymorphic variants in absorption, distribution, metabolism, and excretion (ADME) genes. Despite the high relevance of this issue, few studies have focused on the inter-individual variability in ADME genes in MM risk. To identify new MM susceptibility loci, we performed an extended candidate gene approach by comparing high-throughput genotyping data of 1936 markers in 231 ADME genes on 64 MM patients and 59 controls from the CEU population. Differences in genotype and allele frequencies were validated using an internal control group of 35 non-cancer samples from the same geographic area as the patient group. We detected an association between MM risk and ADH1B rs1229984 (OR = 3.78; 95% CI, 1.18–12.13; p = 0.0282), PPARD rs6937483 (OR = 3.27; 95% CI, 1.01–10.56; p = 0.0479), SLC28A1 rs8187737 (OR = 11.33; 95% CI, 1.43–89.59; p = 0.005), SLC28A2 rs1060896 (OR = 6.58; 95% CI, 1.42–30.43; p = 0.0072), SLC29A1 rs8187630 (OR = 3.27; 95% CI, 1.01–10.56; p = 0.0479), and ALDH3A2 rs72547554 (OR = 2.46; 95% CI, 0.64–9.40; p = 0.0293). The prognostic value of these genes in MM was investigated in two public datasets showing that shorter overall survival was associated with low expression of ADH1B and SLC28A1. In conclusion, our proof-of-concept findings provide novel insights into the genetic bases of MM susceptibility.
cfDNA and DNases: New Biomarkers of Sepsis in Preterm Neonates—A Pilot Study
Authors: Lenz Moritz , Maiberger Thomas , Armbrust Lina , Kiwit Antonia , Von der Wense Axel , Reinshagen Konrad , Elrod Julia , Boettcher Michael
Published: January 2022

Introduction: An early and accurate diagnosis of early onset neonatal sepsis (EONS) and late onset neonatal sepsis (LONS) is essential to improve the outcome of this devastating conditions. Especially, preterm infants are at risk. Reliable biomarkers are rare, clinical decision-making depends on clinical appearance and multiple laboratory findings. Markers of NET formation and NET turnover might improve diagnostic precision. Aim of this study was to evaluate the diagnostic value of NETs in sepsis diagnosis in neonatal preterm infants. Methods: Plasma samples of neonatal preterm infants with suspected sepsis were collected. Blood samples were assayed for markers of NET formation and NET turnover: cfDNA, DNase1, nucleosome, NE, and H3Cit. All clinical findings, values of laboratory markers, and epidemiological characteristics were collected retrospectively. Two subpopulations were created to divide EONS from LONS. EMA sepsis criteria for neonatal sepsis were used to generate a sepsis group (EMA positive) and a control group (EMA negative). Results: A total of 31 preterm neonates with suspected sepsis were included. Out of these, nine patients met the criteria for sepsis according to EMA. Regarding early onset neonatal sepsis (3 EONS vs. 10 controls), cfDNA, DNase I, nucleosome, and CRP were elevated significantly. H3Cit and NE did not show any significant elevations. In the late onset sepsis collective (6 LONS vs. 12 controls), cfDNA, DNase I, and CRP differed significantly compared to control group.
Phylogeny, Structure, Functions, and Role of AIRE in the Formation of T-Cell Subsets
Authors: Shevyrev Daniil , Tereshchenko Valeriy , Kozlov Vladimir , Sennikov Sergey
Published: January 2022

It is well known that the most important feature of adaptive immunity is the specificity that provides highly precise recognition of the self, altered-self, and non-self. Due to the high specificity of antigen recognition, the adaptive immune system participates in the maintenance of genetic homeostasis, supports multicellularity, and protects an organism from different pathogens at a qualitatively different level than innate immunity. This seemingly simple property is based on millions of years of evolution that led to the formation of diversification mechanisms of antigen-recognizing receptors and later to the emergence of a system of presentation of the self and non-self antigens. The latter could have a crucial significance because the presentation of nearly complete diversity of auto-antigens in the thymus allows for the “calibration” of the forming repertoires of T-cells for the recognition of self, altered-self, and non-self antigens that are presented on the periphery. The central role in this process belongs to promiscuous gene expression by the thymic epithelial cells that express nearly the whole spectrum of proteins encoded in the genome, meanwhile maintaining their cellular identity. This complex mechanism requires strict control that is executed by several transcription factors. One of the most important of them is AIRE. This noncanonical transcription factor not only regulates the processes of differentiation and expression of peripheral tissue-specific antigens in the thymic medullar epithelial cells but also controls intercellular interactions in the thymus. Besides, it participates in an increase in the diversity and transfer of presented antigens and thus influences the formation of repertoires of maturing thymocytes. Due to these complex effects, AIRE is also called a transcriptional regulator. In this review, we briefly described the history of AIRE discovery, its structure, functions, and role in the formation of antigen-recognizing receptor repertoires, along with other transcription factors. We focused on the phylogenetic prerequisites for the development of modern adaptive immunity and emphasized the importance of the antigen presentation system.
Super-Enhancers Dysregulations in Hematological Malignancies
Authors: Belloucif Yannis , Lobry Camille
Published: January 2022

Hematological malignancies affecting either the lymphoid or the myeloid lineages involve epigenetic mutations or dysregulation in the majority of cases. These epigenetic abnormalities can affect regulatory elements in the genome and, particularly, enhancers. Recently, large regulatory elements known as super-enhancers, initially identified for their critical roles in cell-type specific expression regulation of genes controlling cell identity, have been shown to also be involved in tumorigenesis in many cancer types and hematological malignancies via the regulation of numerous oncogenes, including MYC. In this review, we highlight the existing links between super-enhancers and hematological malignancies, with a particular focus on acute myeloid leukemia, a clonal hematopoietic neoplasm with dismal outcomes, resulting in an uncontrolled proliferation of myeloblasts, abnormally blocked during differentiation and accumulating within the patient’s bone marrow. We report recent works, performed during the last few years, treating this subject and consider the possibility of targeting oncogenic regulatory elements, as well as the effectiveness and limitations reported so far for such strategies.
Impact of Urbanization on Urban Heat Island Intensity in Major Districts of Bangladesh Using Remote Sensing and Geo-Spatial Tools
Authors: Rahman Md. Naimur , Rony Md. Rakib Hasan , Jannat Farhana Akter , Chandra Pal Subodh , Islam Md. Saiful , Alam Edris , Islam Abu Reza Md. Towfiqul
Published: January 2022

Urbanization is closely associated with land use land cover (LULC) changes that correspond to land surface temperature (LST) variation and urban heat island (UHI) intensity. Major districts of Bangladesh have a large population base and commonly lack the resources to manage fast urbanization effects, so any rise in urban temperature influences the population both directly and indirectly. However, little is known about the impact of rapid urbanization on UHI intensity variations during the winter dry period in the major districts of Bangladesh. To this end, we aim to quantify spatiotemporal associations of UHI intensity during the winter period between 2000 and 2019 using remote-sensing and geo-spatial tools. Landsat-8 and Landsat-5 imageries of these major districts during the dry winter period from 2000 to 2020 were used for this purpose, with overall precision varying from 81% to 93%. The results of LULC classification and LST estimation showed the existence of multiple UHIs in all major districts, which showed upward trends, except for the Rajshahi and Rangpur districts. A substantial increase in urban expansion was observed in Barisal > 32%, Mymensingh > 18%, Dhaka > 17%, Chattogram > 14%, and Rangpur > 13%, while a significant decrease in built-up areas was noticed in Sylhet < −1.45% and Rajshahi < −3.72%. We found that large districts have greater UHIs than small districts. High UHI intensities were observed in Mymensingh > 10 °C, Chattogram > 9 °C, and Barisal > 8 °C compared to other districts due to dense population and unplanned urbanization. We identified higher LST (hotspots) zones in all districts to be increased with the urban expansion and bare land. The suburbanized strategy should prioritize the restraint of the high intensity of UHIs. A heterogeneous increase in UHI intensity over all seven districts was found, which might have potential implications for regional climate change. Our study findings will enable policymakers to reduce UHI and the climate change effect in the concerned districts.
Knowledge, Attitude and Practice for Pruritus Management in Physicians and Patients with Diabetes
Authors: Kalra Sanjay , Mittal Asit , Rathod Roheet M. , Pinto Colette , Rathod Rahul , Mane Amey
Published: January 2022

Pruritus is a common dermatological condition observed in patients with diabetes, making it a dermatometabolic condition. Being multiaethiological, pruritis is caused by autoimmune, genetic, infectious and various systemic diseases. The present survey aimed to understand the knowledge, attitude and practice toward pruritus among Indian physicians and patients with diabetes presenting with pruritus. A telephonic, cross-sectional, qualitative survey was conducted among physicians and patients across five cities in India from July–August 2020. An open-ended discussion guide was used for the interview; the data were analyzed to check for common themes and trends. A majority of the consulting physicians (CPs) believed that uncontrolled diabetes is the main causal factor for pruritus in patients with diabetes and reported that currently there are no standard tests or treatment guidelines for its management. CPs emphasized proper monitoring and counseling to overcome current challenges. Patients reported a negative impact of pruritus on their daily activities and quality of life. The survey concluded that poor management of diabetes is one of the main causal factors for patients with diabetes presenting with pruritus in India. CPs emphasized controlling diabetes along with symptomatic treatment. For patients, pruritus has multifaceted effects on their health, overall well-being, and quality of life.
Study on the Solidification Behavior of Inconel617 Electron Beam Cladding NiCoCrAlY: Numerical and Experimental Simulation
Authors: Chen Jian , Liu Hailang , Peng Zhiguo , Tang Jie
Published: January 2022

To better control the Inconel617 electron beam cladding solidification process, a three-dimensional temperature field model was built to simulate the temperature gradient, cooling rate, and solidification rate in the solidification process and take a deep dive into the solidification behavior, as well as the calculation of the solidification characteristic parameters at the edge of the molten pool and then predict the solidification tissue structure. The study shows that the largest temperature gradient occurred in the material thickness direction. The self-cooling effect of the material dominated the solidification of the alloy layer; the cooling rate depended on the high-temperature thermal conductivity of the material and the self-cooling effect of the matrix, and the maximum cooling rate in the bonding zone was 1380 °C/s. The steady-state solidification rate was equal to the moving speed of the heat source; the solidification characteristics of the solidification process at the edge of the molten pool increased with the distance from the surface: the cooling rate decreased from 1421.61 to 623 °C/s, the temperature gradient increased from 0.0723 × 106 to 0.417 × 106, and the solidification rate decreased from 0.01 to 0 m/s. The prediction was made that the small and thin equiaxed crystals are on the top, a thin and short dendritic transition structure in the middle, and relatively coarse dendrites at the bottom. Experiments confirmed that the solidification tissue structure is basically consistent with the simulation law.
Ultra-Narrow Gap Fiber Laser Conduction Welding Technology for 304 Stainless Steel Thick Plates and the Mechanical Properties of Welding Joints
Authors: Zhang Guowei , Yu Feihu
Published: January 2022

The application of thick metal plates is increasing, and the welding problem is becoming more and more prominent. Narrow gap laser welding is one of the important methods, and it is also a research hotspot. The stainless steel thick plates were welded using the ultra-narrow gap fiber laser conduction welding with filler wire. Results show that the ranges of technological parameters for the achievement of the weld seam with no defects are smaller when the gap width is comparatively larger. Using the optimized technological parameters, the butt welding with no defects on the 3 mm gap between two 304 stainless steel plates with 60 mm thickness was achieved through the filling 20 times. This welding method of 304 metal with large thickness is rare in the literature. The tensile strength of the welding joint can be up to 87% of that of the base metal, and the micro-hardness and yield strength of the joint are comparable with those of the base metal.
Silver@mesoporous Anatase TiO2 Core-Shell Nanoparticles and Their Application in Photocatalysis and SERS Sensing
Authors: Min Yuanzhi , Song Gao , Zhou Ling , Wang Xinyue , Liu Pingying , Li Jumei
Published: January 2022

Nanostructured noble metal-semiconductor materials have been attracting increasing attention because of their broad application in the field of environmental remediation, sensing and photocatalysis. In this study, a facile approach for fabricating silver@mesoporousanataseTiO2 (Ag@mTiO2) core-shell nanoparticles employing sol-gel and hydrothermal reaction is demonstrated. The Ag@mTiO2nanoparticles display excellent surface-enhanced Raman scattering (SERS) sensitivity and they can detect the methylene blue (MB) molecules with the concentration of as low as 10−8 M. They also exhibit outstanding photocatalytic activity compared with mTiO2, due to the efficient separation and recombination restrain of electron–hole pairs under ultraviolet light. The Ag@mTiO2nanoparticles also present good stability and they can achieve recyclable photocatalytic degradation experiments for five times without loss of activity. Subsequently, the nanoparticles with dual functions were successfully used to in situ monitor the photodegradation process of MB aqueous solution. These results, demonstrating the multifunctional Ag@mTiO2 nanoparticles, hold promising applications for simultaneous SERS analysis and the removal of dye pollutants in environmental field.
Microstructure and High-Temperature Performance of High K-Doped Tungsten Fibers Used as Reinforcement of Tungsten Matrix
Authors: Jiang Xiangcao , Song Jiupeng , Peng Fusheng , Guo Donghong , Fang Yijin , Dai Shaowei , Zhu Bingcan
Published: January 2022

Tungsten (W) fiber-reinforced tungsten (Wf/W) composite with ultra-high strength and high-temperature resistance is considered an attractive candidate material for plasma-facing materials (PFM) in future fusion reactors. The main component of Wf/W composite is tungsten wire, which is obtained through powder metallurgy and the drawing process. In this paper, high potassium (K)-doped tungsten wires with 98 ppm of K and 61 ppm of impurities are prepared using traditional and optimized processing technologies, respectively, and a comparative study with conventional K-doped tungsten wires with 83 ppm of K and 80 ppm of impurities is conducted. The high-temperature mechanical properties as well as the microstructure’s evolution of the prepared tungsten wires are investigated. The results show that the high-temperature performance of K-doped tungsten wires is improved by increasing the K content and by simultaneously reducing the impurities. By adopting small compression deformation and low-temperature processing technology, the high-temperature performance of high K-doped tungsten wires can be further improved. A microstructure analysis indicates that the excellent high-temperature performance is attributed to a combination of the small K bubble size, high K bubble number density, and long K bubble string, which are produced through optimization of the processing technology. A study on the processing technology and the performance of tungsten wires with a high K content and a high purity can provide important information regarding Wf/W composites.
Development of a Novel Low-Silver Cu-P Brazing Filler Metal Bearing Sn
Authors: Wu Jie , Xue Songbai , Luo Qingcheng
Published: January 2022

The flame brazing of H62 brass using a novel, low-silver Cu-P brazing filler metal was investigated in this study. The effect of the addition of a trace amount of Sn on the microstructure and properties of Cu-7P-1Ag filler metals was analyzed by means of X-ray diffractometer, scanning electron microscopy and energy dispersive spectrometer. The addition of trace Sn led to a decrease in the solidus and liquidus temperatures of Cu-7P-1Ag filler metals. Meanwhile, the spreading performance of the filler metals on a H62 brass substrate was improved. The microstructure of the low-silver, Cu-P brazing filler metal was mainly composed of α-Ag solid solution, α-Cu solid solution and Cu3P; an increase of Sn content led to the transformation of the microstructure of the joints from a block to a lamellar structure. When the Sn content was 0.5 wt. %, the shear strength of the joint at room temperature reached 348 MPa, and the fracture morphologies changed from a cleavage to a quasi-cleavage structure.
Nano/Micro and Bio-Inspired Materials on Wide-Bandgap-Semiconductor-Based Optoelectronic/Power Devices
Authors: Reddy Mallem Siva Pratap
Published: January 2022

This Special Issue on “Nano/Micro and Bio-Inspired Materials on Wide-Bandgap-Semiconductor-Based Optoelectronic/Power Devices” is a collection of 20 original articles dedicated to theoretical and experimental research works providing new insights and practical findings in the field of solid-state technology-related topics [...]
Effect of Irradiation with Low-Energy He2+ Ions on Degradation of Structural, Strength and Heat-Conducting Properties of BeO Ceramics
Authors: Zdorovets Maxim V. , Shlimas Dmitriy I. , Kozlovskiy Artem L. , Borgekov Daryn B.
Published: January 2022

The paper is devoted to the study of radiation-induced damage kinetics in beryllium oxide ceramics under irradiation with low-energy helium ions with fluences of 1015–1018 ion/cm2. It was revealed that at irradiation fluences above 1017 ion/cm2, a decrease in radiation-induced damage formation and accumulation rate is observed, which indicates the saturation effect. At the same time, the main mechanisms of structural changes caused by irradiation at these fluences are amorphization processes and dislocation density increase, while at fluences of 1015–1016 ion/cm2, the main mechanisms of structural changes are due to the reorientation of crystallites and a change in texture, with a small contribution of crystal lattice distorting factors. It was discovered that the radiation-induced damage accumulation as well as an implanted helium concentration increase leads to the surface layer destruction, which is expressed in the ceramic surface hardness and wear resistance deterioration. It was determined that with irradiation fluences of 1015–1016 ion/cm2, the decrease in thermal conductivity is minimal and is within the measurement error, while an increase in the irradiation fluence above 1017 ion/cm2 leads to an increase in heat losses by more than 10%.
Comparative Study of Bulk and Nanoengineered Doped ZnSe
Authors: Setera Brett , Su Ching-Hua , Arnold Bradley , Choa Fow-Sen , Kelly Lisa , Sood Rachit , Singh N.
Published: January 2022

Chromium- and cobalt-doped zinc selenide nanoparticles were synthesized using a low-temperature reactive solution growth method. The morphological and optical characteristics were compared to those of doped zinc selenide (ZnSe) bulk crystals grown by the physical vapor transport (PVT) method. We observed agglomeration of particles; however, the thioglycerol capping agent has been shown to limit particle grain growth and agglomeration. This process enables doping by addition of chromium and cobalt salts in the solution. A slightly longer refluxing time was required to achieve cobalt doping as compared with chromium doping due to lower refluxing temperature. The nanoparticle growth process showed an average particle size of approximately 300 nm for both Cr- and Co-doped zinc selenide. The optical characterization of Co:ZnSe is ongoing; however, preliminary results showed a very high bandgap compared to that of pure ZnSe bulk crystal. Additionally, Co:ZnSe has an order of magnitude higher fluorescence intensity compared to bulk Cr:ZnSe samples.
In-Depth Optical Analysis of Zn(Al)O Mixed Metal Oxide Film-Based Zn/Al-Layered Double Hydroxide for TCO Application
Authors: Salih Ethar Yahya , Ramizy Asmiet , Aldaghri Osamah , Mohd Sabri Mohd Faizul , Madkhali Nawal , Alinad Tarfah , Ibnaouf Khalid Hassan , Eisa Mohamed Hassan
Published: January 2022

In this article, an in-depth optical investigation of Zn(Al)O-mixed metal oxide (MMO) film using Zn/Al-layered double hydroxide (LDH) was elucidated through co-precipitation and spin coating techniques. The field emission scanning electron microscopy (FE-SEM) analysis revealed the occurrence of a vertically aligned sheet-like structure with a thickness of 60 nm for pristine LDH, which further reduced to 45 nm after calcination at 300 °C. Additionally, pristine LDH showed multiple optical bandgaps of 5.18, 3.6, and 3.2 eV. Moreover, a good agreement of the obtained optical bandgaps was attained between both utilized methods, ultraviolet-visible light (UV-Vis), and photoluminescence (PL) spectroscopies. The optical bandgap decreased at higher calcination temperatures, which indicates the active role of the applied post-fabrication process on the optical profile of the deposited MMO film/s. The demonstrated transmittance spectra of the deposited MMO films exhibited a transparency between 85% and 95%; this indicates the usefulness and consistency of the proposed film for transparent conductive oxide (TCO) based optoelectronic applications.
Impact of Pre-Treatment NLR and Other Hematologic Biomarkers on the Outcomes of Early-Stage Non-Small-Cell Lung Cancer Treated with Stereotactic Body Radiation Therapy
Authors: Aduquaye Marina , Dube Sheen , Bashir Bashir , Chowdhury Amitava , Ahmed Naseer , Leylek Ahmet , Kim Julian , Lambert Pascal , Bucher Oliver , Hunter William , Sivananthan Gokulan , Koul Rashmi , Rathod Shrinivas
Published: January 2022

Introduction: We evaluated the association of pre-treatment immunologic biomarkers on the outcomes of early-stage non-small-cell lung cancer (NSCLC) patients treated with stereotactic body radiation therapy (SBRT). Materials and methods: In this retrospective study, all newly diagnosed early-stage NSCLC treated with SBRT between January 2010 and December 2017 were screened and included for further analysis. The pre-treatment neutrophil-lymphocyte ratio (NLR), monocyte lymphocyte ratio (MLR), and platelet-lymphocyte ratio (PLR) were calculated. Overall survival (OS) and recurrence-free survival (RFS) were estimated by Kaplan–Meier. Multivariable models were constructed to determine the impact of different biomarkers and the Akaike information criterion (AIC), index of adequacy, and scaled Brier scores were calculated. Results: A total of 72 patients were identified and 61 were included in final analysis. The median neutrophil count at baseline was 5.4 × 109/L (IQR: 4.17–7.05 × 109/L). Median lymphocyte count was 1.63 × 109/L (IQR: 1.29–2.10 × 109/L), median monocyte count was 0.65 × 109/L (IQR: 0.54–0.83 × 109/L), median platelet count was 260.0 × 109/L (IQR: 211.0–302.0 × 109/L). The median NLR was 3.42 (IQR: 2.38–5.04), median MLR was 0.39 (IQR: 0.31–0.53), and median PLR was 156.4 (IQR: 117.2–197.5). On multivariable regression a higher NLR was associated with worse OS (p = 0.01; HR-1.26; 95% CI 1.04–1.53). The delta AIC between the two multivariable models was 3.4, suggesting a moderate impact of NLR on OS. On multivariable analysis, higher NLR was associated with poor RFS (p = 0.001; NLR^1 HR 0.36; 0.17–0.78; NLR^2 HR-1.16; 95% CI 1.06–1.26) with a nonlinear relationship. The delta AIC between the two multivariable models was 16.2, suggesting a strong impact of NLR on RFS. In our cohort, MLR and PLR were not associated with RFS or OS in multivariable models. Conclusions: Our study suggests NLR, as a biomarker of systemic inflammation, is an independent prognostic factor for OS and RFS. The nonlinear relationship with RFS may indicate a suitable immunological environment is needed for optimal SBRT action and tumoricidal mechanisms. These findings require further validation in independent cohorts.
Patterns of Genetic Variation in the Eisenia nordenskioldi Complex (Oligochaeta: Lumbricidae) along an Elevation Gradient in Northern China
Authors: Zhang Yufeng , Zhang Yiming , Wu Hui , Li Congsheng , Aspe Nonillon M. , Wu Donghui
Published: January 2022

Eisenia nordenskioldi is the dominant earthworm species in many tundra and boreal habitats. Nothing is known about the genetic diversity of this species along the elevation gradient in China. This study sampled 28 individuals in the E. nordenskioldi complex from Wuling Mountain, northern China, to examine their external morphology and genetic diversity. Mt. Wuling is the southern limit of the distribution of the E. nordenskioldi complex. The specimens from Mt. Wuling were classified into three groups along an elevation gradient. Mismatch distribution analysis suggested that the Pleistocene glaciations possibly did not significantly affect the distribution of earthworm species in this region. We also found that elevation affected the genetic diversity, but not the external morphology of E. nordenskioldi. Given the altitudinal genetic diversity within the E. nordenskioldi complex, the phylogeography of this species provides important information for the zoogeographic reconstruction of the mountains in northern China. With the relatively limited sample size, the result is not conclusive, and further studies need to be conducted in the future to verify the results.
Visualization of Dialysis-Related Amyloid Arthropathy on 18F-FDG PET-CT Scan
Authors: Cheon Miju , Yoo Jang
Published: January 2022

We report a case of dialysis-related amyloid arthropathy in a patient with end-stage renal disease. It presented in our patient as moderately increased FDG uptake in the amyloid deposition in the periarticular tissues and eroding into adjacent bones.
Blood Flow Analysis of the Great Saphenous Vein in the Su-Pine Position in Clinical Manifestations of Varicose Veins of Different Severities: Application of Phase-Contrast Magnetic Resonance Imaging Data
Authors: Tseng Yuan-Hsi , Chen Chien-Wei , Wong Min-Yi , Yang Teng-Yao , Lin Yu-Hui , Lin Bor-Shyh , Huang Yao-Kuang
Published: January 2022

The progression of clinical manifestations of lower-limb varicose veins remains unclear. This study investigated changes in lower-limb venous blood flow using phase-contrast magnetic resonance angiography. Data were collected on veins from 141 legs. We compared legs with and without varicose veins and related symptoms and examined varying levels of varicose vein symptom severity. Legs without varicose veins exhibited a lower absolute stroke volume (ASV, p < 0.01) and mean flux (MF, p = 0.03) for the great saphenous vein (GSV) compared with legs with symptomatic varicose veins. Legs with asymptomatic varicose veins exhibited lower MF for the GSV (p = 0.02) compared with legs with symptomatic varicose veins. Among legs with varicose veins, asymptomatic legs exhibited lower ASV (p = 0.03) and MF (p = 0.046) for the GSV compared with legs that exhibited skin changes or ulcers; however, no significant differences were observed between legs presenting with discomfort or edema and legs with skin changes or ulcers, and between legs presenting with discomfort or edema and asymptomatic legs. In conclusion, in the supine position, increased blood flow rate and blood flow volume in the GSV were associated with symptomatic varicose veins and increased symptom severity.
Acoustic Neurinoma with Synchronous Ipsilateral Cerebellopontine Angle Lipoma: A Case Report and Review of the Literature
Authors: Kanaya Takahiro , Murai Yasuo , Yui Kanako , Sato Shun , Morita Akio
Published: January 2022

Lipomas of the cerebellopontine angle (CPA) and internal auditory canal (IAC) are relatively rare tumors. Acoustic neurinoma is the most common tumor in this location, which often causes hearing loss, vertigo, and tinnitus. Occasionally, this tumor compresses the brainstem, prompting surgical resection. Lipomas in this area may cause symptoms similar to neurinoma. However, they are not considered for surgical treatment because their removal may result in several additional deficits. Conservative therapy and repeated magnetic resonance imaging examinations for CPA/IAC lipomas are standard measures for preserving cranial nerve function. Herein, we report a case of acoustic neurinoma and CPA lipoma occurring in close proximity to each other ipsilaterally. The main symptom was hearing loss without facial nerve paralysis. Therefore, facial nerve injury had to be avoided. Considering the anatomical relationships among the tumors, cranial nerves, and CPA/IAC lipoma, we performed total surgical removal of the acoustic neurinoma. We intentionally left the lipoma untreated, which enabled facial nerve preservation. This report may be a useful reference for the differential diagnosis of similar cases in the future.
The Predictive Role of NLR, d-NLR, MLR, and SIRI in COVID-19 Mortality
Authors: Citu Cosmin , Gorun Florin , Motoc Andrei , Sas Ioan , Gorun Oana Maria , Burlea Bogdan , Tuta-Sas Ioana , Tomescu Larisa , Neamtu Radu , Malita Daniel , Citu Ioana Mihaela
Published: January 2022

(1) Background: Since its discovery, COVID-19 has caused more than 256 million cases, with a cumulative death toll of more than 5.1 million, worldwide. Early identification of patients at high risk of mortality is of great importance in saving the lives of COVID-19 patients. The study aims to assess the utility of various inflammatory markers in predicting mortality among hospitalized patients with COVID-19. (2) Methods: A retrospective observational study was conducted among 108 patients with laboratory-confirmed COVID-19 hospitalized between 1 May 2021 and 31 October 2021 at Municipal Emergency Clinical Hospital of Timisoara, Romania. Blood cell counts at admission were used to obtain NLR, dNLR, MLR, PLR, SII, and SIRI. The association of inflammatory index and mortality was assessed via Kaplan–Maier curves univariate Cox regression and binominal logistic regression. (3) Results: The median age was 63.31 ± 14.83, the rate of in-hospital death being 15.7%. The optimal cutoff for NLR, dNLR, MLR, and SIRI was 9.1, 9.6, 0.69, and 2.2. AUC for PLR and SII had no statistically significant discriminatory value. The binary logistic regression identified elevated NLR (aOR = 4.14), dNLR (aOR = 14.09), and MLR (aOR = 3.29), as independent factors for poor clinical outcome of COVID-19. (4) Conclusions: NLR, dNLR, MLR have significant predictive value in COVID-19 mortality.
Deep-Learning Segmentation of Epicardial Adipose Tissue Using Four-Chamber Cardiac Magnetic Resonance Imaging
Authors: Daudé Pierre , Ancel Patricia , Confort Gouny Sylviane , Jacquier Alexis , Kober Frank , Dutour Anne , Bernard Monique , Gaborit Bénédicte , Rapacchi Stanislas
Published: January 2022

In magnetic resonance imaging (MRI), epicardial adipose tissue (EAT) overload remains often overlooked due to tedious manual contouring in images. Automated four-chamber EAT area quantification was proposed, leveraging deep-learning segmentation using multi-frame fully convolutional networks (FCN). The investigation involved 100 subjects—comprising healthy, obese, and diabetic patients—who underwent 3T cardiac cine MRI, optimized U-Net and FCN (noted FCNB) were trained on three consecutive cine frames for segmentation of central frame using dice loss. Networks were trained using 4-fold cross-validation (n = 80) and evaluated on an independent dataset (n = 20). Segmentation performances were compared to inter-intra observer bias with dice (DSC) and relative surface error (RSE). Both systole and diastole four-chamber area were correlated with total EAT volume (r = 0.77 and 0.74 respectively). Networks’ performances were equivalent to inter-observers’ bias (EAT: DSCInter = 0.76, DSCU-Net = 0.77, DSCFCNB = 0.76). U-net outperformed (p < 0.0001) FCNB on all metrics. Eventually, proposed multi-frame U-Net provided automated EAT area quantification with a 14.2% precision for the clinically relevant upper three quarters of EAT area range, scaling patients’ risk of EAT overload with 70% accuracy. Exploiting multi-frame U-Net in standard cine provided automated EAT quantification over a wide range of EAT quantities. The method is made available to the community through a FSLeyes plugin.
GroEL Chaperonin-Based Assay for Early Diagnosis of Scrub Typhus
Authors: Indrawattana Nitaya , Aiumurai Pisinee , Sae-lim Nawannaporn , Seesuay Watee , Reamtong Onrapak , Chongsa-nguan Manas , Chaicumpa Wanpen , Sookrung Nitat
Published: January 2022

A point-of-care diagnostic for early and rapid diagnosis of scrub typhus caused by Orientia tsutsugamushi is required for prompt and proper treatment of patients presenting with undifferentiated febrile illnesses. In this study, an immunochromatographic antigen detection test kit (ICT AgTK) that targets the highly conserved O. tsutsugamushi 60 kDa GroEL chaperonin (heat shock protein 60) was developed. E. coli-derived recombinant GroEL expressed from DNA coding for the consensus sequence of 32 GroEL gene sequences extracted from the GenBank database was used to immunize rabbits and mice. Rabbit polyclonal antibodies (pAb) were used for preparing a gold-pAb conjugate, and the rGroEL-specific mouse monoclonal antibody was used as the antigen detection reagent at the ICT test line. In-house validation revealed that the ICT AgTK gave 85, 100 and 95% diagnostic sensitivity, specificity and accuracy, respectively, compared to the combined clinical features and standard IFA when tested on 40 frozen serum samples. The test kits correctly identified 10 scrub typhus samples out of 15 fresh plasma/buffy coat samples of patients with febrile illnesses. For independent laboratory validation, the ICT AgTK was sent to one provincial hospital. The ICT AgTK utilized by the hospital medical technologist correctly identified six scrub typhus samples out of 20 serum samples of patients with fever, as confirmed by specific IgM/IgG detection by IFA. The ICT AgTK is easy to perform with rapid turn-around time. It has the potential to be used as an important tool for on-site and early scrub typhus diagnosis by allowing testing of freshly collected samples (serum, plasma or buffy coat), especially in resource-limited healthcare settings.
Tumor Hypoxia Heterogeneity Affects Radiotherapy: Inverse-Percolation Shell-Model Monte Carlo Simulations
Authors: Dimou Argyris , Argyrakis Panos , Kopelman Raoul
Published: January 2022

Tumor hypoxia was discovered a century ago, and the interference of hypoxia with all radiotherapies is well known. Here, we demonstrate the potentially extreme effects of hypoxia heterogeneity on radiotherapy and combination radiochemotherapy. We observe that there is a decrease in hypoxia from tumor periphery to tumor center, due to oxygen diffusion, resulting in a gradient of radiative cell-kill probability, mathematically expressed as a probability gradient of occupied space removal. The radiotherapy-induced break-up of the tumor/TME network is modeled by the physics model of inverse percolation in a shell-like medium, using Monte Carlo simulations. The different shells now have different probabilities of space removal, spanning from higher probability in the periphery to lower probability in the center of the tumor. Mathematical results regarding the variability of the critical percolation concentration show an increase in the critical threshold with the applied increase in the probability of space removal. Such an observation will have an important medical implication: a much larger than expected radiation dose is needed for a tumor breakup enabling successful follow-up chemotherapy. Information on the TME’s hypoxia heterogeneity, as shown here with the numerical percolation model, may enable personalized precision radiation oncology therapy.
Deep Q-Learning-Based Neural Network with Privacy Preservation Method for Secure Data Transmission in Internet of Things (IoT) Healthcare Application
Authors: Kathamuthu Nirmala Devi , Chinnamuthu Annadurai , Iruthayanathan Nelson , Ramachandran Manikandan , Gandomi Amir H.
Published: January 2022

The healthcare industry is being transformed by the Internet of Things (IoT), as it provides wide connectivity among physicians, medical devices, clinical and nursing staff, and patients to simplify the task of real-time monitoring. As the network is vast and heterogeneous, opportunities and challenges are presented in gathering and sharing information. Focusing on patient information such as health status, medical devices used by such patients must be protected to ensure safety and privacy. Healthcare information is confidentially shared among experts for analyzing healthcare and to provide treatment on time for patients. Cryptographic and biometric systems are widely used, including deep-learning (DL) techniques to authenticate and detect anomalies, andprovide security for medical systems. As sensors in the network are energy-restricted devices, security and efficiency must be balanced, which is the most important concept to be considered while deploying a security system based on deep-learning approaches. Hence, in this work, an innovative framework, the deep Q-learning-based neural network with privacy preservation method (DQ-NNPP), was designed to protect data transmission from external threats with less encryption and decryption time. This method is used to process patient data, which reduces network traffic. This process also reduces the cost and error of communication. Comparatively, the proposed model outperformed some standard approaches, such as thesecure and anonymous biometric based user authentication scheme (SAB-UAS), MSCryptoNet, and privacy-preserving disease prediction (PPDP). Specifically, the proposed method achieved accuracy of 93.74%, sensitivity of 92%, specificity of 92.1%, communication overhead of 67.08%, 58.72 ms encryption time, and 62.72 ms decryption time.
Exploring the Measurement Lab Open Dataset for Internet Performance Evaluation: The German Internet Landscape
Authors: Lübben Ralf , Misfeld Nico
Published: January 2022

The Measurement Lab (MLab) provides a large and open collection of Internet performance measurements. We make use of it to look at the state of the German Internet by a structured analysis, in which we carve out expressive results from the dataset to identify busy hours and days, the impact of server locations and congestion control protocols, and compare Internet service providers. Moreover, we examine the impact of the COVID-19 lockdown in Germany. We observe that only parts of the Internet show a performance degradation at the beginning of the lockdown and that a large impact in performance depends on the network the servers are located in. Furthermore, the evolution of congestion control algorithms is reflected by performance improvements. For our analysis, we focus on the busy hours. From the end-user perspective, this time is of most interest to identify if the network can support challenging services such as video streaming or cloud gaming at these intervals.
A Survey on LoRaWAN Technology: Recent Trends, Opportunities, Simulation Tools and Future Directions
Authors: Almuhaya Mukarram A. M. , Jabbar Waheb A. , Sulaiman Noorazliza , Abdulmalek Suliman
Published: January 2022

Low-power wide-area network (LPWAN) technologies play a pivotal role in IoT applications, owing to their capability to meet the key IoT requirements (e.g., long range, low cost, small data volumes, massive device number, and low energy consumption). Between all obtainable LPWAN technologies, long-range wide-area network (LoRaWAN) technology has attracted much interest from both industry and academia due to networking autonomous architecture and an open standard specification. This paper presents a comparative review of five selected driving LPWAN technologies, including NB-IoT, SigFox, Telensa, Ingenu (RPMA), and LoRa/LoRaWAN. The comparison shows that LoRa/LoRaWAN and SigFox surpass other technologies in terms of device lifetime, network capacity, adaptive data rate, and cost. In contrast, NB-IoT technology excels in latency and quality of service. Furthermore, we present a technical overview of LoRa/LoRaWAN technology by considering its main features, opportunities, and open issues. We also compare the most important simulation tools for investigating and analyzing LoRa/LoRaWAN network performance that has been developed recently. Then, we introduce a comparative evaluation of LoRa simulators to highlight their features. Furthermore, we classify the recent efforts to improve LoRa/LoRaWAN performance in terms of energy consumption, pure data extraction rate, network scalability, network coverage, quality of service, and security. Finally, although we focus more on LoRa/LoRaWAN issues and solutions, we introduce guidance and directions for future research on LPWAN technologies.
Gas Hold-Up and Mass Transfer in a Vessel with an Unsteady Rotating Concave Blade Impeller
Authors: Frankiewicz Sebastian , Woziwodzki Szymon
Published: January 2022

The steady mixing of gas-liquid systems is used where a large development of the interfacial area is required. However, the presence of gas in the liquid reduces the efficiency of mass transfer by reducing the mixing power, due to the creation of gas formations behind the impeller blades and the reduction in density. The efficiency of mass transfer can be increased by using a concave blade impeller or unsteady mixing. Mass transfer efficiency studies for these impellers and unsteady mixing are limited. This paper presents an analysis of the influence of the impeller construction on the gas hold-up and volumetric mass transfer coefficient kLa. Impellers with a different number of concave blades, and with alternatively arranged concave blades, were analyzed. The obtained results were compared with the standard flat blade turbine. The obtained results indicate that the arrangement of the concave blades has the greatest effect on reducing the gas hold-up and kLa. Higher values were obtained for the four-bladed and six-bladed impellers. A comparison of the gas hold-up rate for the unsteady and steady mixing has shown that for steady mixing greater gas hold-up is achieved. The volumetric mass transfer coefficient for unsteady mixing is also greater compared to steady mixing, indicating greater efficiency in mass transfer.