bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Remission of chronic low back pain and oral dysesthesia comorbid with attention deficit/hyperactivity disorder by treatment with atomoxetine and pramipexole
Authors: Kasahara Satoshi , Kato Yuichi , Takahashi Miwako , Matsudaira Ko , Sato Naoko , Niwa Shin-Ichi , Momose Toshimitsu , Uchida Kanji
Published: June 2023

Introduction

Oral dysesthesia is a disease characterized by pain and/or abnormal sensations in the oral region, without any organic abnormality. Its symptoms include pain, and it is considered to be a disorder associated with idiopathic oral-facial pain. It is also known that idiopathic oral-facial pain tends to coexist with chronic musculoskeletal pain, including low back pain, even before its onset. Such coexisting idiopathic pain conditions are also called chronic overlapping pain conditions (COPCs). In general, COPCs are often refractory to treatment. Recently, it has been reported that attention deficit hyperactivity disorder (ADHD) is associated with many COPCs, such as pain in the facial and lower back regions and so on. However, there are no reports of (1) ADHD as a comorbidity with oral dysesthesia (OD) or (2) of the therapeutic effects of ADHD medications or dopamine agonists on low back pain and OD or an (3) evaluation of cerebral blood flow over time after treatment with these medications for OD and low back pain.

Case Presentation

In this study, we report the case of an 80-year-old man with OD and chronic low back pain that persisted for more than 25 years. His OD and chronic back pain were refractory to standard treatment, prevented him from continuing work, and tended to be exacerbated by conflicts in his relationship with his son. In recent years, ADHD has often been found to be comorbid with chronic pain, and ADHD medications have been reported to improve chronic pain as well. The patient was confirmed to have undiagnosed ADHD and was treated with the ADHD medication atomoxetine and dopamine agonist pramipexole, which dramatically improved his OD, chronic back pain, and cognitive function. Furthermore, along the course of treatment, there was improvement in cerebral blood flow in his prefrontal cortex, which was thought to reflect improved function in the region. Consequently, he was able to resume work and improve his family relationships.

Conclusion

Therefore, in the cases of ODs and COPCs, screening for ADHD and, if ADHD is diagnosed, ADHD medications or dopamine agonists may be considered.
Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis
Authors: Zhang Hailong , Zhou Jingwen , Kou Xiaoyue , Liu Yuqi , Zhao Xiaonan , Qin Guochen , Wang Mingyu , Qian Guangtao , Li Wen , Huang Yongshun , Wang Xiaoting , Zhao Zhenjie , Li Shuang , Wu Xiaoqian , Jiang Lixi , Feng Xianzhong , Zhu Jian-Kang , Li Lixin
Published: June 2023

SYP71, a plant-specific Qc-SNARE with multiple subcellular localization, is essential for symbiotic nitrogen fixation in nodules in Lotus, and is implicated in plant resistance to pathogenesis in rice, wheat and soybean. Arabidopsis SYP71 is proposed to participate in multiple membrane fusion steps during secretion. To date, the molecular mechanism underlying SYP71 regulation on plant development remains elusive. In this study, we clarified that AtSYP71 is essential for plant development and stress response, using techniques of cell biology, molecular biology, biochemistry, genetics, and transcriptomics. AtSYP71-knockout mutant atsyp71-1 was lethal at early development stage due to the failure of root elongation and albinism of the leaves. AtSYP71-knockdown mutants, atsyp71-2 and atsyp71-3, had short roots, delayed early development, and altered stress response. The cell wall structure and components changed significantly in atsyp71-2 due to disrupted cell wall biosynthesis and dynamics. Reactive oxygen species homeostasis and pH homeostasis were also collapsed in atsyp71-2. All these defects were likely resulted from blocked secretion pathway in the mutants. Strikingly, change of pH value significantly affected ROS homeostasis in atsyp71-2, suggesting interconnection between ROS and pH homeostasis. Furthermore, we identified AtSYP71 partners and propose that AtSYP71 forms distinct SNARE complexes to mediate multiple membrane fusion steps in secretory pathway. Our findings suggest that AtSYP71 plays an essential role in plant development and stress response via regulating pH homeostasis through secretory pathway.
Limits to the Anthropocene: geopolitical conflict or cooperative governance?
Authors: Scheffran Jürgen
Published: June 2023

In the Anthropocene the world is facing an acceleration of human growth and its impact on nature. The expansionist world order which emerged from Europe since colonial times is reaching multiple limits (environmental, economic, social, political and scientific-technical), increasing marginal costs and risks which trigger multiple crises, conflicts and catastrophes that challenge this world order. Alternative futures range from a collapse of human civilization to geopolitical power competition and conflict between rivals to disruptive technical innovations and systemic transformation of the economy and society within natural boundaries. In response to geopolitical conflicts and their consequences, such as climate change and the Russia-Ukraine war, efforts of cooperative governance can help to mitigate, adapt to and manage complex crisis landscapes. Instead of an epochal turn (Zeitenwende) for arms race and war, more promising are sustainable climate protection and a peaceful energy transition within planetary boundaries. To further prevent escalating and mutually enforcing crisis dynamics and geopolitical conflicts in the Anthropocene, cooperative governance needs to adjust to the world's complexity and move from a negative nexus of problems to a positive nexus of solutions. The interaction between geopolitics and governance and the transition from risk cascades to synergies is discussed for the energy-security nexus and the climate-conflict-migration nexus. Energy conflicts can be contained by diminishing land competition and biodiversity loss, as well as risky dependencies on strategic raw materials and conflict minerals. Measures for a sustainable energy transition include energy efficiency and conservation, renewable energy and decarbonization, a circular economy and nature-based solutions. To prevent risk multiplication in the climate-conflict-migration nexus, synergies in climate, migration and security policy facilitate integrative solutions for a socio-ecological transformation based on mitigation and adaptation, conflict resolution and environmental peacebuilding, aiming for a mutual enforcement of sustainability and peace.
Why are human animacy judgments continuous rather than categorical? A computational modeling approach
Authors: Westbury Chris
Published: June 2023

Introduction

The concept of animacy is often taken as a basic natural concept, in part I because most cases seem unambiguous. Most entities either are or are not animate. However, human animacy judgments do not reflect this binary classification. They suggest that there are borderline cases, such as virus, amoeba, fly, and imaginary beings (giant, dragon, god). Moreover, human roles (professor, mother, girlfriend) are consistently recognized as animate by far less than 100% of human judges.

Method

In this paper, I use computational modeling to identify features associated with human animacy judgments, modeling human animacy and living/non-living judgments using both bottom-up predictors (the principal components from a word embedding model) and top-down predictors (cosine distances from the names of animate categories).

Results

The results suggest that human animacy judgments may be relying on information obtained from imperfect estimates of category membership that are reflected in the word embedding models. Models using cosine distance from category names mirror human judgments in distinguishing strongly between humans (estimated lower animacy by the measure) and other animals (estimated higher animacy by the measure).

Discussion

These results are consistent with a family resemblance approach to the apparently categorical concept of animacy.
The influence of leader humor on employee creativity: from the perspective of employee voice
Authors: Cao Yi , Zhou Keqiucheng , Wang Yijiang , Hou Yubo , Miao Rentao
Published: June 2023

Creativity is a primary factor in driving transformational change. This study explored the impact of leader humor on employee creativity (incremental and radical creativity) from the perspective of employee voice. Data were collected from 812 Chinese employees through multipoint surveys. Through the surveys, we found that (1) leader humor has a significant positive effect on employee incremental and radical creativity; (2) promotive/prohibitive voice mediates the relationship between leader humor and employee incremental/radical creativity separately; (3) contradictory thinking moderates the effect of leader humor on prohibitive voice and further moderates the indirect effect of leader humor on radical creativity; and (4) the moderated mediation model mainly applies to state-owned enterprises rather than private-owned enterprises. The theoretical and practical implications of these findings are discussed.
High stress related to COVID-19 among health workers in the Plateau Central healthcare region (BURKINA FASO): a cross-sectional study
Authors: Traoré Solo , Dahourou Désiré Lucien , Paré Boyo Constant , Lompo Yemboado Diedonné , Kaboré Wendlassida Josiane , Ouédraogo Wind-La-Sida Abd-El-Aziz , Kambou Datouo Thomas , Salou Wenddinda Rabbaly Adeline , Kaboré Delphin , Ouédraogo Abdoulaye , Sia Siaka , Zorné Mady , Ouoba Boezemwendé , Guira Oumar
Published: June 2023

Background

The COVID-19 pandemic challenged the mental wellbeing of health workers. The objective of this study was to assess health workers' perceived stress during the response to COVID-19 in the Central Plateau region (Burkina Faso).

Methods

We conducted a cross-sectional study of health workers in the Central Plateau health region from September 20 to October 20, 2021. Agents' perceived stress was assessed by the Perceived Stress Scale (PSS-10). Factors associated with high stress (PSS-10 score ≥ 27) were identified by logistic regression.

Results

A total of 272 officers participated in the survey. The mean PSS-10 score was 29.3 points (standard deviation: 6.2). Three out of ten agents (68%) had a high level of stress. The main sources of stress were the risk of being exposed to contamination (70%) and being the source of contamination (78%). Working at the referral health center [adjusted odds ratio (aOR): 2.29; 95% confidence interval (95% CI): 1.19–4.41], the hospital as the main source of COVID-19 information (aOR: 1.17; 95% CI: 1.01-3.04), fear of COVID-19 patients being managed at one's center (aOR: 1.8; 95% CI: 1.06–3.07) were factors associated with high health worker stress levels during the first wave of COVID-19.

Conclusion

The COVID-19 pandemic caused high stress among health care workers in Burkina Faso. Psychological support for health center workers in responding to future epidemics would improve their mental health.
Passivity based nonlinear model predictive control (PNMPC) of multi-robot systems for space applications
Authors: Kalaycioglu Serdar , De Ruiter Anton
Published: June 2023

In the past 2 decades, there has been increasing interest in autonomous multi-robot systems for space use. They can assemble space structures and provide services for other space assets. The utmost significance lies in the performance, stability, and robustness of these space operations. By considering system dynamics and constraints, the Model Predictive Control (MPC) framework optimizes performance. Unlike other methods, standard MPC can offer greater robustness due to its receding horizon nature. However, current literature on MPC application to space robotics primarily focuses on linear models, which is not suitable for highly non-linear multi-robot systems. Although Nonlinear MPC (NMPC) shows promise for free-floating space manipulators, current NMPC applications are limited to unconstrained non-linear systems and do not guarantee closed-loop stability. This paper introduces a novel approach to NMPC using the concept of passivity to multi-robot systems for space applications. By utilizing a passivity-based state constraint and a terminal storage function, the proposed PNMPC scheme ensures closed-loop stability and a superior performance. Therefore, this approach offers an alternative method to the control Lyapunov function for control of non-linear multi-robot space systems and applications, as stability and passivity exhibit a close relationship. Finally, this paper demonstrates that the benefits of passivity-based concepts and NMPC can be combined into a single NMPC scheme that maintains the advantages of each, including closed-loop stability through passivity and good performance through one-line optimization in NMPC.
Feasibility of Roux-en-Y Gastric Bypass with the novel robotic platform HUGO™ RAS
Authors: Raffaelli Marco , Voloudakis Nikolaos , Pennestrì Francesco , Gallucci Pierpaolo , Modesti Cristina , Salvi Giulia , Greco Francesco , Ciccoritti Luigi
Published: June 2023

Introduction

Robotic assisted surgery is a rapidly developing field of minimally invasive bariatric surgery in the last 20 years. Its wide diffusion has led to the development and standardization of robotic assisted approaches for bariatric operations. In this study, we present the first four Roux-en-Y Gastric Bypass (RYGB) operations performed with the new Hugo™ RAS system (Medtronic, Minneapolis, MN, USA).

Methods

In January and February 2023, 4 consecutive patients scheduled for minimal invasive Roux-en-Y-Bypass were selected and underwent the procedure robotic-assisted with the new platform. No exclusion criteria were applied.

Results

Four patients, two females and two males, underwent RYGB with a median BMI of 40 Kg/m2 (range: 36–46) and diabetes mellitus in two cases. The median docking time was 8 min (range: 7–8.5) and the median console time was 127.5 min (range: 95–150). A description of the operating theatre, robotic arms and docking setup is provided. Procedures were performed without intraoperative complications and no conversion to laparoscopy or open surgery was noted. No additional ports were needed to be placed. System's function and docking were uneventful. No early post-operative complications were observed.

Conclusions

Based on our initial experience, RYGB with the Hugo™ RAS system is feasible. This study provides the configurations necessary to perform RYGB with the Hugo™ RAS system as well as general information and insights from our preliminary experience.
On-farm antimicrobial usage in commercial turkey production in the United States, 2013–2021
Authors: Singer Randall S. , Schrag Nora F. D. , Ricke Isabel , Apley Michael D.
Published: June 2023

A key component of antimicrobial stewardship is the ability to collect antimicrobial use data and ultimately use this information to ensure that administrations are necessary and effective. National antimicrobial sales data cannot help in this capacity because the data lack context, for example, details concerning target species and disease indication. The objective of this study was to continue the development of a system for collecting flock-level on-farm antimicrobial use data from the U.S. turkey industry and to have it be representative of national turkey production in the U.S. This study utilized a public-private partnership to enable collection and protection of sensitive flock-level data from an extremely large industry while releasing deidentified and aggregated information regarding the details of antimicrobial use on U.S. turkey farms over time. Participation was voluntary. Data were collected for the period 2013 through 2021 and are reported on a calendar year basis. Using production statistics from USDA:NASS as a denominator, the data supplied by participating companies represented approximately 67.3% of turkey production in the U.S. in 2013, approximately 69.1% in 2017, and approximately 71.4% in 2021. The data that were submitted for 2021 are based on approximately 149,000,192 turkeys slaughtered and 4,929,773,506 pounds liveweight produced. Detailed prescription records representing approximately 60–70% of the birds were available for the 2018–2021 dataset. The estimated percentage of turkey poults placed that received hatchery antimicrobials decreased from 96.9% in 2013 to 40.5% in 2021. The use of in-feed antimicrobials was practically eliminated, with in-feed tetracycline being the only medically important antimicrobial used in 2021. Use of in-feed tetracyclines decreased approximately 80% between 2013 and 2021. Water-soluble antimicrobial use declined over the study period. Between 2013 and 2021, water-soluble penicillin use decreased approximately 41% but water-soluble tetracycline use increased approximately 22%. Key diseases that were treated with water-soluble antimicrobials included bacterial poult enteritis and clostridial dermatitis. Efforts to reduce the incidence of these diseases would reduce the need for antimicrobial therapy, thereby enabling continued decreases in antimicrobial use without sacrificing animal welfare. However, this will require an investment in research to find efficacious and cost-effective mitigation strategies.
Grimace scale assessment during Citrobacter rodentium inflammation and colitis development in laboratory mice
Authors: Peppermüller Pia Pascale , Gehring Jonathan , Zentrich Eva , Bleich André , Häger Christine , Buettner Manuela
Published: June 2023

Introduction

Bacterial infections and chronic intestinal inflammations triggered by genetic susceptibility, environment or an imbalance in the intestinal microbiome are usually long-lasting and painful diseases in which the development and maintenance of these various intestinal inflammations is not yet fully understood, research is still needed. This still requires the use of animal models and is subject to the refinement principle of the 3Rs, to minimize suffering or pain perceived by the animals. With regard to this, the present study aimed at the recognition of pain using the mouse grimace scale (MGS) during chronic intestinal colitis due to dextran sodium sulfate (DSS) treatment or after infection with Citrobacter rodentium.

Methods

In this study 56 animals were included which were divided into 2 experimental groups: 1. chronic intestinal inflammation (n = 9) and 2. acute intestinal inflammation (with (n = 23) and without (n = 24) C. rodentium infection). Before the induction of intestinal inflammation in one of the animal models, mice underwent an abdominal surgery and the live MGS from the cage side and a clinical score were assessed before (bsl) and after 2, 4, 6, 8, 24, and 48 hours.

Results

The highest clinical score as well as the highest live MGS was detected 2 hours after surgery and almost no sign of pain or severity were detected after 24 and 48 hours. Eight weeks after abdominal surgery B6-Il4/Il10-/- mice were treated with DSS to trigger chronic intestinal colitis. During the acute phase as well as the chronic phase of the experiment, the live MGS and a clinical score were evaluated. The clinical score increased after DSS administration due to weight loss of the animals but no change of the live MGS was observed. In the second C57BL/6J mouse model, after infection with C. rodentium the clinical score increased but again, no increased score values in the live MGS was detectable.

Discussion

In conclusion, the live MGS detected post-operative pain, but indicated no pain during DSS-induced colitis or C. rodentium infection. In contrast, clinical scoring and here especially the weight loss revealed a decreased wellbeing due to surgery and intestinal inflammation.
Conceptual Design of Hybrid Aerial Vehicle for Venus Exploration
Authors: Rosales Jesus , Miller Addison , Nunez Edgar , Gross Andreas , Chanover Nancy
Published: June 2023

The conceptual design of a hybrid aerial vehicle for the exploration of the upper Venus atmosphere is presented. The vehicle will float like a balloon and harvest solar energy which is stored in batteries. The neutral buoyancy reduces the energy consumption and makes the vehicle robust and durable. Energy stored in the batteries can be used for powered flight with good horizontal and vertical mobility to explore aspects of the atmosphere. The vehicle is intended to operate near 55.3 km altitude and to explore the cloud layer of the planet. The vehicle takes its inspiration from the Stingray inflatable wing by Prospective Concepts. Based on a trade study, the wing span was set to 25 m. Equations are developed for the altitude, gas and skin temperature, and skin stress during neutrally buoyant flight. To keep the equations in a simplified analytical form, the complex compartmentalized gas pockets of the vehicle are lumped into a single gas sphere. The equations take into account the volumetric expansion of the structure and the requirement that the differential pressure needs to be large enough to allow for brief periods of powered flight without significant structural deformation. An aerodynamic analysis provides the lift and drag coefficient curves and indicates that the vehicle is pitch-stable. A powered flight analysis shows that an airspeed of 30 m/s can be maintained for 31 min at 55 km and 69 min at 69 km altitude.
Closed-Loop Control of Transonic Buffet Using Active Shock Control Bump
Authors: Deng Feng , Zhang Shenghua , Qin Ning
Published: June 2023

At transonic flight conditions, the buffet caused by the shockwave/boundary-layer interaction can degrade aircraft performance and even threaten their safety. In this paper, a closed-loop control using an active shock control bump (SCB) has been proposed to suppress the buffet on a supercritical airfoil flying at transonic speeds. A closed-loop control law is designed by using the lift coefficient as the feedback signal and using the bump height as the control variable. The unsteady numerical simulations show that the buffet can be effectively suppressed by an optimal combination of the parameters of the control law, namely the gain and the delay time. Furthermore, the buffet control effectiveness is still acceptably constrained by a prescribed maximum bump height, which is believed to be practically important. In addition to being able to achieve both wave drag reduction and buffet alleviation, the active SCB is less sensitive to the parameters of the control law and has a shorter response time in comparison with the reference active trailing edge flap.
Effect of Adoption of Climate-Smart-Agriculture Technologies on Cereal Production, Food Security and Food Diversity in Central Mali
Authors: Sissoko Penda , Guindo Sékou Sala , Togola Sadio , Dembélé Bakary Désiré , Grimsby Lars Kåre , Aune Jens B.
Published: June 2023

Over a period of two years, this study analyzes the effect on food security of introducing new technologies through farmer field schools in the project “Adapting Agriculture and Livestock to Climate Change” (ACC). A household survey was conducted to compare 125 households in villages that were part of the project to 79 households in villages that were not part of the project. These two groups were compared regarding cereal surplus production, the number of food-secure months and food diversity. The data were analyzed using analysis of variance, a two-sample t-test and boxplots. The project was implemented in the semiarid regions of Segou and Koulikoro in Mali. The technologies introduced were microdosing of mineral fertilizer, seed priming, new cereal varieties, horticulture, poultry and goat production, assisted natural regeneration and ridging. Microdosing of mineral fertilizer and seed priming was adopted by more than 85% of the households in the ACC villages. Grain yields of pearl millet, sorghum and maize increased by 418, 429 and 673 kg/ha, respectively, (p < 0.0001) due to seed priming and microdosing. This resulted in a cereal surplus of 756 kg in the ACC households, while the surplus in the non-ACC households was 161 kg. In addition, the ACC households were food secure for two months longer than the non-ACC households and consumed food from one more food group than the non-ACC households. This study shows that, despite the difficult situation in Mali, it is possible to improve food security by introducing improved technologies.
YOLO-Sp: A Novel Transformer-Based Deep Learning Model for Achnatherum splendens Detection
Authors: Zhang Yuzhuo , Wang Tianyi , You Yong , Wang Decheng , Zhang Dongyan , Lv Yuchan , Lu Mengyuan , Zhang Xingshan
Published: June 2023

The growth of Achnatherum splendens (A. splendens) inhibits the growth of dominant grassland herbaceous species, resulting in a loss of grassland biomass and a worsening of the grassland ecological environment. Therefore, it is crucial to identify the dynamic development of A. splendens adequately. This study intended to offer a transformer-based A. splendens detection model named YOLO-Sp through ground-based visible spectrum proximal sensing images. YOLO-Sp achieved 98.4% and 95.4% AP values in object detection and image segmentation for A. splendens, respectively, outperforming previous SOTA algorithms. The research indicated that Transformer had great potential for monitoring A. splendens. Under identical training settings, the AP value of YOLO-Sp was greater by more than 5% than that of YOLOv5. The model’s average accuracy was 98.6% in trials conducted at genuine test sites. The experiment revealed that factors such as the amount of light, the degree of grass growth, and the camera resolution would affect the detection accuracy. This study could contribute to the monitoring and assessing grass plant biomass in grasslands.
Circadian Variation of Peripheral Blood Cells in Horses Maintained in Different Environmental and Management Conditions
Authors: Aragona Francesca , Arfuso Francesca , Fazio Francesco , De Caro Salvatore , Giudice Elisabetta , Monteverde Vincenzo , Piccione Giuseppe , Giannetto Claudia
Published: June 2023

The aim of our study was to analyze circadian rhythm of the hematological profile of horses housed in a loose box and paddock during the different seasons (spring, summer, autumn, and winter). Blood samples were performed every 4 h for 48 consecutive hours. Red blood cells (RBCs), hemoglobin (HGB), hematocrit (HCT), white blood cells (WBCs), platelets (PLTs), and leukocyte subpopulations (neutrophils, basophils, eosinophils, lymphocytes, and monocytes) were analyzed, and, at the same time, environmental conditions were recorded. A statistically significant effect of housing conditions (p < 0.0001) was observed on all hematological values except for WBC during winter and for neutrophils (p < 0.0001) during spring and autumn. A statistically significant effect of season (p < 0.0001) was found for RBC, HCT, and PLT and for all leukocyte cells (p < 0.0001) except for basophils. The single Cosinor method revealed a daily rhythm of hematological parameters during spring in both groups, and a daily rhythm for lymphocytes and neutrophils was observed during spring and summer in horses kept in a loose box and during winter in horses housed in a paddock. Our results revealed that the response of the immune system is regulated by circadian physiology. Knowledge of the periodic temporal structure of mammals should be considered when evaluating animals’ adaptation to temporizations imposed by the environment.
Molecular Survey and Genetic Characteristics of Vector-Borne Pathogens in Domestic Dogs from Four Regions of China
Authors: Yin Fangyuan , Guo Chuanjiang , Li Dong , Tian Zhuojia , Li Facai
Published: June 2023

Canine vector-borne diseases are widely distributed around the world. They are transmitted by arthropods, and many seriously threaten the health of animals and humans. In China, our knowledge of Ehrlichia, Hepatozoon, and Mycoplasma species circulating in dogs is still poorly understood. Therefore, the aim of this study was to understand the prevalence and genetic characteristics of canine Ehrlichia spp., Hepatozoon spp., and Mycoplasma spp. in Chongqing (southwest), Fujian (southeast), Shandong (southeast), and Hubei (central) Provinces of China. Blood samples from healthy pet dogs were processed to detect Ehrlichia, Hepatozoon, and Mycoplasma DNA with PCR. Haplotype and phylogenetic analyses were performed on 18S rRNA sequences. Among 306 dogs, no Ehrlichia spp. or Mycoplasma spp. were detected, whereas one Hepatozoon sp. was detected in 10 (3.27%) of the animals. Only Hepatozoon canis was identified and was endemic to Chongqing (2.46%) and Hubei (8.77%). A haplotype analysis identified eight haplotypes among the H. canis isolates. A phylogenetic analysis showed that the H. canis isolates in this study clustered into four clades, together with isolates from different countries and hosts, forming a large group that was clearly separate from other Hepatozoon species. These findings provided new information on the epidemiological characteristics of canine vector-borne diseases in China and will be helpful in the development of efficient measures to safeguard the health and well-being of companion animals and their owners.
Hoof Expansion, Deformation, and Surface Strains Vary with Horseshoe Nail Positions
Authors: Dahl Vanessa E. , Singer Ellen R. , Garcia Tanya C. , Hawkins David A. , Stover Susan M.
Published: June 2023

Racehorses are susceptible to underrun heel hoof conformation. Racehorses are often shod with nails placed toward the heel. It is unknown if palmar nails restrict or alter hoof deformation in a manner that could promote the development of underrun heel conformation over time with repeated loading. To determine how the addition of palmar nails affects heel deformation during limb loading, hoof expansion and hoof wall deformations were quantified using rosette strain gauges and kinematic markers during in the vitro limb loading of cadaveric limbs that simulated midstance for walk, trot, and canter loads. Nail treatments used to attach a horseshoe to the hoof included: toe nails (T), toe and quarter nails (TQ), and toe, quarter, and heel nails (TQH). The effects of nail treatment on heel expansion and hoof wall deformations were assessed using repeated measures analysis of variance (p < 0.05). Nails placed palmar to the quarters of the hoof decreased heel expansion (p < 0.001). Heel nails resulted in the largest changes in hoof wall principal strain directions distally. The application of nails palmar to the hoof quarters alters hoof wall deformation during limb loading. The continued loading of the hoof with palmer nails could alter hoof conformation over time.
First Toxicological Analysis of the Pufferfish Sphoeroides pachygaster Collected in Italian Waters (Strait of Sicily): Role of Citizens Science in Monitoring Toxic Marine Species
Authors: Malloggi Chiara , Rizzo Biagio , Giusti Alice , Guardone Lisa , Gasperetti Laura , Dall’Ara Sonia , Armani Andrea
Published: June 2023

Pufferfish (Tetraodontidae) inhabiting the Mediterranean Sea may represent an emerging public health risk due to the possible accumulation of marine neurotoxins such as tetrodotoxin (TTXs) and saxitoxin (STXs) in their tissues. In this study, the presence of pufferfish species in the Strait of Sicily (Lampedusa Island, Italy) was investigated using a citizen science (CS) approach, involving local fishermen. Samples (liver, intestine, gonads, muscle, skin) from 20 specimens were sent to the National Reference Laboratory on Marine Biotoxins for TTXs detection using a validated HILIC-MS/MS method on fish tissue. The presence of STXs was also screened in part of the specimens. Overall, 56 specimens identified as Sphoeroides pachygaster (Müller &Troschel, 1848) were collected. Data on their total length, body weight, fishing method and catch area (with relative depth temperature and salinity) were analyzed and compared with the S. pachygaster records reported in literature which were updated to 2022. All the analysed tissues were found to be negative for both TTXs and STXs. CS played an essential role in monitoring potentially toxic marine species in this investigation. Outcomes from this study, which is the first investigating S. pachygaster toxicity in Italian waters, may provide useful data for the proper assessment of this emerging risk.
Characterization of Chromatin Accessibility in Fetal Bovine Chondrocytes
Authors: Zhang Qi , Li Qian , Wang Yahui , Zhang Yapeng , Peng Ruiqi , Wang Zezhao , Zhu Bo , Xu Lingyang , Gao Xue , Chen Yan , Gao Huijiang , Hu Junwei , Qian Cong , Ma Minghao , Duan Rui , Li Junya , Zhang Lupei
Published: June 2023

Despite significant advances of the bovine epigenome investigation, new evidence for the epigenetic basis of fetal cartilage development remains lacking. In this study, the chondrocytes were isolated from long bone tissues of bovine fetuses at 90 days. The Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) and transcriptome sequencing (RNA-seq) were used to characterize gene expression and chromatin accessibility profile in bovine chondrocytes. A total of 9686 open chromatin regions in bovine fetal chondrocytes were identified and 45% of the peaks were enriched in the promoter regions. Then, all peaks were annotated to the nearest gene for Gene Ontology (GO) and Kyoto Encylopaedia of Genes and Genomes (KEGG) analysis. Growth and development-related processes such as amide biosynthesis process (GO: 0043604) and translation regulation (GO: 006417) were enriched in the GO analysis. The KEGG analysis enriched endoplasmic reticulum protein processing signal pathway, TGF-β signaling pathway and cell cycle pathway, which are closely related to protein synthesis and processing during cell proliferation. Active transcription factors (TFs) were enriched by ATAC-seq, and were fully verified with gene expression levels obtained by RNA-seq. Among the top50 TFs from footprint analysis, known or potential cartilage development-related transcription factors FOS, FOSL2 and NFY were found. Overall, our data provide a theoretical basis for further determining the regulatory mechanism of cartilage development in bovine.
Evaluation of Yeast Hydrolysate in a Low-Fishmeal Diet for Whiteleg Shrimp (Litopenaeus vannamei)
Authors: Hamidoghli Ali , Lee Yein , Hwang Soyeon , Choi Wonsuk , Choi Youn-Hee , Bai Sungchul C.
Published: June 2023

An eight-week feeding trial was performed to evaluate the effects of yeast hydrolysate (YH) supplementation in a low-fishmeal diet on the growth, immune responses, intestinal histology and disease resistance of whiteleg shrimp (Litopenaeus vannamei). Five experimental diets were produced by supplementing YH at 0 (CON), 0.5 (YH0.5), 1 (YH1), 2 (YH2) and 4 (YH4) % to a basal diet containing 10% fishmeal and compared with a positive control with 25% fishmeal (FM25). Shrimp with an initial average weight of 0.43 ± 0.005 g (mean ± SD) were stocked in 18 tanks and fed the experimental diets (38% protein and 8% lipid) four times a day. Results showed that shrimp fed the FM25 diet exhibited significantly higher final body weight, weight gain, specific growth rate and protein efficiency ratio than those fed CON, YH0.5, YH1 and YH2 diets (p < 0.05). However, there were no significant differences between shrimp fed the YH4 and FM25 diets (p > 0.05). In addition, there were no significant differences in whole-body proximate composition, hemolymph biochemical parameters and non-specific immune responses among treatments. Intestinal villi length and muscular layer thickness of shrimp fed the YH4 and FM25 diets were significantly higher than the other groups. At the end of the bacterial (Vibrio parahaemolyticus) challenge test, shrimp fed YH4 and FM25 diets showed a significantly higher survival rate than those of shrimp fed CON, YH0.5 and YH1 (p < 0.05). These results suggest that supplementing 4% YH in diet containing 10% fishmeal could beneficially influence growth, intestinal morphology and disease resistance of whiteleg shrimp.
Genetic Diversity and Population Structure of Bulgarian Autochthonous Sheep Breeds Revealed by Microsatellite Analysis
Authors: Mihailova Yanka , Rusanov Krasimir , Rusanova Mila , Vassileva Pavlina , Atanassov Ivan , Nikolov Vasil , Todorovska Elena G.
Published: June 2023

This study attempts to provide a deeper insight into the current genetic status of 12 Bulgarian autochthonous sheep breeds using microsatellite (SSR) markers. A total of 600 individuals from 50 flocks were analyzed using a panel of 13 SSR markers. In total, 228 alleles were found in the studied microsatellite loci. The mean number of alleles, the effective number of alleles, and the polymorphic information content (PIC) values per locus were 17.54, 5.250, and 0.799, respectively. The expected heterozygosity (He) for all breeds ranged from 0.70 to 0.82. The within-population heterozygote deficit (Fis) varied from −0.03 to 0.1, reflecting significant levels for 10 of the 12 breeds. The average genetic differentiation (Fst) was 0.046, revealing a low discrimination between the breeds. The genetic distance, principal coordinate analysis, and the structure analysis showed that two of the studied breeds—Local Stara Zagora/SZ/ and Local Karnobat/MK/—were the most distinct sheep populations. The Bayesian clustering approach suggested poor breed differentiation for the remaining 10 sheep breeds. The results suggest that proper management strategies and specific breeding policies need to be implemented in Bulgaria to avoid the intermixing of breeds and to reduce the erosion of breed purity observed in some breeds.
Recombinant Dense Granule Protein (NcGRA4) Is a Novel Serological Marker for Neospora caninum Infection in Goats
Authors: Udonsom Ruenruetai , Mahittikorn Aongart , Prachasuphap Apichai , Jongpitisub Kodcharad , Dhepakson Panadda , Jirapattharasate Charoonluk
Published: June 2023

Neospora caninum is widely recognised as one of the most significant causes of abortion in cattle, with infections also occurring in sheep and goats. To prevent and control animal neosporosis, it is crucial to develop sensitive and specific methods for detecting N. caninum infection. Recently, several recombinant proteins have been utilised in serological assays for the diagnosis of neosporosis. In this study, we used commercial gene synthesis to produce dense granular antigen 4 (NcGRA4) recombinant protein. NcGRA4 plasmids were expressed in the Escherichia coli system and then purified. The purified recombinant protein was analysed using sodium dodecyl sulphate–polyacrylamide gel electrophoresis. To evaluate the diagnostic potential of recombinant NcGRA4 protein, we tested 214 serum samples from goat farms via indirect enzyme-linked immunosorbent assay (iELISA) and compared the results to those from the indirect fluorescent antibody test (IFAT). Western blotting analysis revealed a single NcGRA4 band with an expected molecular weight of 32 kDa. The specific IgG against N. caninum was detected in 34.1% and 35% of samples evaluated by NcGRA4 iELISA and IFAT, respectively. The sensitivity and specificity of the NcGRA4 iELISA were 71.6% and 86.3%, respectively, when compared with the results from IFAT. Our results demonstrate that a recombinant protein that can be used to detect animal neosporosis can be produced using a synthetic NcGRA4 gene. Overall, recombinant NcGRA4 shows promise as a sensitive and specific serological marker for identifying target IgG in goat samples.
Fabrication and Characterisation of the Cytotoxic and Antibacterial Properties of Chitosan-Cerium Oxide Porous Scaffolds
Authors: Yildizbakan Lemiha , Iqbal Neelam , Ganguly Payal , Kumi-Barimah Eric , Do Thuy , Jones Elena , Giannoudis Peter V. , Jha Animesh
Published: June 2023

Bone damage arising from fractures or trauma frequently results in infection, impeding the healing process and leading to complications. To overcome this challenge, we engineered highly porous chitosan scaffolds (S1, S2, and S3) by incorporating 30 (wt)% iron-doped dicalcium phosphate dihydrate (Fe-DCPD) minerals and different concentrations of cerium oxide nanoparticles (CeO2) (10 (wt)%, 20 (wt)%, and 30 (wt)%) using the lyophilisation technique. The scaffolds were specifically designed for the controlled release of antibacterial agents and were systematically characterised by utilising Raman spectroscopy, X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy methodologies. Alterations in the physicochemical properties, encompassing pore size, swelling behaviour, degradation kinetics, and antibacterial characteristics, were observed with the escalating CeO2 concentrations. Scaffold cytotoxicity and its impact on human bone marrow mesenchymal stem cell (BM-MSCs) proliferation were assessed employing the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. The synthesised scaffolds represent a promising approach for addressing complications associated with bone damage by fostering tissue regeneration and mitigating infection risks. All scaffold variants exhibited inhibitory effects on bacterial growth against Staphylococcus aureus and Escherichia coli strains. The scaffolds manifested negligible cytotoxic effects while enhancing antibacterial properties, indicating their potential for reducing infection risks in the context of bone injuries.
Combating Bacterial Biofilms: Current and Emerging Antibiofilm Strategies for Treating Persistent Infections
Authors: Abdelhamid Ahmed G. , Yousef Ahmed E.
Published: June 2023

Biofilms are intricate multicellular structures created by microorganisms on living (biotic) or nonliving (abiotic) surfaces. Medically, biofilms often lead to persistent infections, increased antibiotic resistance, and recurrence of infections. In this review, we highlighted the clinical problem associated with biofilm infections and focused on current and emerging antibiofilm strategies. These strategies are often directed at disrupting quorum sensing, which is crucial for biofilm formation, preventing bacterial adhesion to surfaces, impeding bacterial aggregation in viscous mucus layers, degrading the extracellular polymeric matrix, and developing nanoparticle-based antimicrobial drug complexes which target persistent cells within the biofilm core. It is important to acknowledge, however, that the use of antibiofilm agents faces obstacles, such as limited effectiveness in vivo, potential cytotoxicity to host cells, and propensity to elicit resistance in targeted biofilm-forming microbes. Emerging next generation antibiofilm strategies, which rely on multipronged approaches, were highlighted, and these benefit from current advances in nanotechnology, synthetic biology, and antimicrobial drug discovery. The assessment of current antibiofilm mitigation approaches, as presented here, could guide future initiatives toward innovative antibiofilm therapeutic strategies. Enhancing the efficacy and specificity of some emerging antibiofilm strategies via careful investigations, under conditions that closely mimic biofilm characteristics within the human body, could bridge the gap between laboratory research and practical application.
Ocular Antibiotic Utilisation across Aotearoa/New Zealand
Authors: Cheung Isabella M. Y. , Horsburgh Simon , Ziaei Mohammed , Gokul Akilesh
Published: June 2023

Ocular antibiotics are integral to the prevention and treatment of bacterial ocular infections. This study aimed to describe their utilisation across New Zealand according to patient and healthcare factors. Every subsidy-eligible community dispensing of ocular chloramphenicol, fusidic acid and ciprofloxacin in New Zealand, between 2010 and 2019, was included in this analysis. Number of dispensings/1000 population/year was quantified, stratified by patient age and urban/non-urban health districts. Dispensing rates by ethnicity were determined and were age adjusted. The proportion of dispensings by socioeconomic deprivation quintile was also determined. Chloramphenicol was the most commonly dispensed antibiotic; however, its utilisation decreased over time. Ciprofloxacin use was higher in children, while chloramphenicol use was higher in older patients. Ciprofloxacin usage was higher among Māori and Pasifika ethnicities, while fusidic acid use was lower. Chloramphenicol usage was higher among Pasifika. Antibiotic utilisation was higher in urban health districts, and in the most deprived quintile; both were most marked with ciprofloxacin. The utilisation of publicly funded ocular antibiotics across New Zealand varied between patient subgroups. These findings will help improve the prevention, management and outcomes of bacterial ocular infections, and support wider initiatives in antibiotic stewardship and medicine access equity.
Antimicrobial Stewardship in COVID-19 Patients: Those Who Sow Will Reap Even through Hard Times
Authors: Sibani Marcella , Canziani Lorenzo Maria , Tonolli Chiara , Armellini Maddalena , Carrara Elena , Mazzaferri Fulvia , Conti Michela ,   SAVE Working Group, Mazzariol Annarita , Micheletto Claudio , Dalbeni Andrea , Girelli Domenico , Tacconelli Evelina
Published: June 2023

Background: Since the SARS-CoV-2 pandemic emerged, antimicrobial stewardship (AS) activities need to be diverted into COVID-19 management. Methods: In order to assess the impact of COVID-19 on AS activities, we analyzed changes in antibiotic consumption in moderate-to-severe COVID-19 patients admitted to four units in a tertiary-care hospital across three COVID-19 waves. The AS program was introduced at the hospital in 2018. During the first wave, COVID-19 forced the complete withdrawal of hospital AS activities. In the second wave, antibiotic guidance calibration for COVID-19 patients was implemented in all units, with enhanced stewardship activities in Units 1, 2, and 3 (intervention units). In a controlled before and after study, antimicrobial usage during the three waves of the COVID-19 pandemic was compared to the 12-month prepandemic unit (Unit 4 acted as the control). Antibiotic consumption data were analyzed as the overall consumption, stratified by the World Health Organization AWaRe classification, and expressed as defined-daily-dose (DDD) and days-of-therapy (DOT) per 1000 patient-day (PD). Results: In the first wave, the overall normalized DOT in units 2–4 significantly exceeded the 2019 level (2019: 587 DOT/1000 PD ± 42.6; Unit 2: 836 ± 77.1; Unit 3: 684 ± 122.3; Unit 4: 872, ± 162.6; p < 0.05). After the introduction of AS activities, consumption decreased in the intervention units to a significantly lower level when compared to 2019 (Unit 1: 498 DOT/1000 PD ± 49; Unit 2: 232 ± 95.7; Unit 3: 382 ± 96.9; p < 0.05). Antimicrobial stewardship activities resulted in a decreased amount of total antibiotic consumption over time and positively affected the watch class and piperacillin-tazobactam use in the involved units. Conclusions: During a pandemic, the implementation of calibrated AS activities represents a sound investment in avoiding inappropriate antibiotic therapy.
A 2022 Update on Computational Approaches to the Discovery and Design of Antimicrobial Peptides
Authors: Agüero-Chapin Guillermin , Antunes Agostinho , Marrero-Ponce Yovani
Published: June 2023

The antimicrobial resistance process has been accelerated by the over-prescription and misuse of antibiotics [...]