bluuebunny/crossref_metadata_2025 huggingface dataset:
Autophagic Heterogeneity in Gastric Adenocarcinoma
Authors: Yoon Ju-Yoon , Brezden-Masley Christine , Streutker Catherine J.
Published: March 2021

Background and Aim

Gastric/gastroesophageal junction (GEJ) adenocarcinoma is a heterogeneous disease, with various etiologies and with tumors encompassing a spectrum of histologic and molecular subtypes. “Autophagy” includes two related but distinct homeostatic processes that promote cell survival under adverse conditions, namely macro- and chaperone-mediated autophagy. There is increasing evidence of the roles autophagy may play in tumorigenesis.

Methods

Autophagic pathways were examined in the context of the heterogeneity intrinsic to gastric/GEJ adenocarcinoma, utilizing immunohistochemistry targeting specific proteins within the pathways (p62, LAMP2A, LC3B). We examined whole sections of normal and dysplastic gastric mucosa, as well as a tissue microarray of adenocarcinomas.

Results

Dysplastic gastric epithelium was marked by frequent nuclear p62 and aberrant LAMP2A expression compared to normal. Examining the pattern of LC3B/cytoplasmic p62 immuno-reactivity in gastric adenocarcinoma demonstrated a predominant pattern of LC3BHigh/p62High staining (56/86, 65.1%), which has been previously associated with active, but impaired macroautophagy. There were no statistically significant associations seen between LC3B/cytoplasmic p62 staining patterns with tumor grade, histotype, or approximated TCGA molecular subtype. LAMP2A and nuclear p62 and staining patterns were also heterogeneous across the cohort, but with no statistically significant associations seen. The prognostic significance of the three proteins was limited, however high nuclear p62 levels were associated with worse overall survival (log-rank p-value = 0.0396).

Conclusion

Our data demonstrate the dynamic nature of autophagic proteins in the gastric epithelium, and we expand the biological heterogeneity observed in gastric/GEJ adenocarcinoma to include autophagy.
Novel Cell-Penetrating Peptides Derived From Scaffold-Attachment- Factor A Inhibits Cancer Cell Proliferation and Survival
Authors: Puvvula Pavan Kumar , Moon Anne M.
Published: March 2021

Scaffold-attachment-factor A (SAFA) has important roles in many normal and pathologic cellular processes but the scope of its function in cancer cells is unknown. Here, we report dominant-negative activity of novel peptides derived from the SAP and RGG-domains of SAFA and their effects on proliferation, survival and the epigenetic landscape in a range of cancer cell types. The RGG-derived peptide dysregulates SAFA binding and regulation of alternatively spliced targets and decreases levels of key spliceosome proteins in a cell-type specific manner. In contrast, the SAP-derived peptide reduces active histone marks, promotes chromatin compaction, and activates the DNA damage response and cell death in a subset of cancer cell types. Our findings reveal an unprecedented function of SAFA-derived peptides in regulating diverse SAFA molecular functions as a tumor suppressive mechanism and demonstrate the potential therapeutic utility of SAFA-peptides in a wide range of cancer cells.
Role of Hyperbaric Oxygenation Plus Hypofractionated Stereotactic Radiotherapy in Recurrent High-Grade Glioma
Authors: Arpa Donatella , Parisi Elisabetta , Ghigi Giulia , Cortesi Annalisa , Longobardi Pasquale , Cenni Patrizia , Pieri Martina , Tontini Luca , Neri Elisa , Micheletti Simona , Ghetti Francesca , Monti Manuela , Foca Flavia , Tesei Anna , Arienti Chiara , Sarnelli Anna , Martinelli Giovanni , Romeo Antonio
Published: March 2021

Background

The presence of hypoxic cells in high-grade glioma (HGG) is one of major reasons for failure of local tumour control with radiotherapy (RT). The use of hyperbaric oxygen therapy (HBO) could help to overcome the problem of oxygen deficiency in poorly oxygenated regions of the tumour. We propose an innovative approach to improve the efficacy of hypofractionated stereotactic radiotherapy (HSRT) after HBO (HBO-RT) for the treatment of recurrent HGG (rHGG) and herein report the results of an ad interim analysis.

Methods

We enrolled a preliminary cohort of 9 adult patients (aged >18 years) with a diagnosis of rHGG. HSRT was administered in daily 5-Gy fractions for 3-5 consecutive days a week. Each fraction was delivered up to maximum of 60 minutes after HBO.

Results

Median follow-up from re-irradiation was 11.6 months (range: 3.2-11.6 months). The disease control rate (DCR) 3 months after HBO-RT was 55.5% (5 patients). Median progression-free survival (mPFS) for all patients was 5.2 months (95%CI: 1.34-NE), while 3-month and 6-month PFS was 55.5% (95%CI: 20.4-80.4) and 27.7% (95%CI: 4.4-59.1), respectively. Median overall survival (mOS) of HBO-RT was 10.7 months (95% CI: 7.7-NE). No acute or late neurologic toxicity >grade (G)2 was observed in 88.88% of patients. One patient developed G3 radionecrosis.

Conclusions

HSRT delivered after HBO appears to be effective for the treatment of rHGG, it could represent an alternative, with low toxicity, to systemic therapies for patients who cannot or refuse to undergo such treatments.

Clinical Trial Registration

www.ClinicalTrials.gov, identifier NCT 03411408.
The Impact of Variant Allele Frequency in EGFR Mutated NSCLC Patients on Targeted Therapy
Authors: Friedlaender Alex , Tsantoulis Petros , Chevallier Mathieu , De Vito Claudio , Addeo Alfredo
Published: March 2021

EGFR mutations represent the most common currently targetable oncogenic driver in non-small cell lung cancer. There has been tremendous progress in targeting this alteration over the course of the last decade, and third generation tyrosine kinase inhibitors offer previously unseen survival rates among these patients. Nonetheless, a better understanding is still needed, as roughly a third of patients do not respond to targeted therapy and there is an important heterogeneity among responders. Allelic frequency, or the variant EGFR allele frequency, corresponds to the fraction of sequencing reads harboring the mutation. The allelic fraction is influenced by the proportion of tumor cells in the sample, the presence of copy number alterations but also, most importantly, by the proportion of cells within the tumor that carry the mutation. Mutations that occur early in tumor evolution, often called clonal or truncal, have a higher allelic frequency than late, subclonal mutations, and are more often drivers of cancer evolution and attractive therapeutic targets. Most, but not all, EGFR mutations are clonal. Although an exact estimate of clonal proportion is hard to derive computationally, the allelic frequency is readily available to clinicians and could be a useful surrogate. We hypothesized that tumors with low allelic frequency of the EGFR mutation will respond less favorably to targeted treatment.
Is Ultra-Short-Term Heart Rate Variability Valid in Non-static Conditions?
Authors: Kim Jin Woong , Seok Hyeon Seok , Shin Hangsik
Published: March 2021

In mobile healthcare, heart rate variability (HRV) is increasingly being used in dynamic patient states. In this situation, shortening of the measurement time is required. This study aimed to validate ultra-short-term HRV in non-static conditions. We conducted electrocardiogram (ECG) measurements at rest, during exercise, and in the post-exercise recovery period in 30 subjects and analyzed ultra-short-term HRV in time and frequency domains by ECG in 10, 30, 60, 120, 180, and 240-s intervals, and compared the values to the 5-min HRV. For statistical analysis, null hypothesis testing, Cohen’s d statistics, Pearson’s correlation coefficient, and Bland-Altman analysis were used, with a statistical significance level of P < 0.05. The feasibility of ultra-short-term HRV and the minimum time required for analysis showed differences in each condition and for each analysis method. If the strict criteria satisfying all the statistical methods were followed, the ultra-short-term HRV could be derived from a from 30 to 240-s length of ECG. However, at least 120 s was required in the post-exercise recovery or exercise conditions, and even ultra-short-term HRV was not measurable in some variables. In contrast, according to the lenient criteria needed to satisfy only one of the statistical criteria, the minimum time required for ultra-short-term HRV analysis was 10–60 s in the resting condition, 10–180 s in the exercise condition, and 10–120 s in the post-exercise recovery condition. In conclusion, the results of this study showed that a longer measurement time was required for ultra-short-term HRV analysis in dynamic conditions. This suggests that the existing ultra-short-term HRV research results derived from the static condition cannot applied to the non-static conditions of daily life and that a criterion specific to the non-static conditions are necessary.
Supramaximal-Exercise Training Improves Heart Rate Variability in Association With Reduced Catecholamine in Obese Adults
Authors: Jabbour Georges , Iancu Horia D.
Published: March 2021

This study investigates the effect of 6 weeks of supramaximal exercise training (SET) on heart rate variability (HRV) and associated factors in sedentary obese (OB) and normal-weight (NW) adults. In this study, 19 OB [22.9 (8.4) years; body mass index (BMI) 33.4 (1.4) kg/m2] and 18 NW [23.2 (4.4) years; BMI 23.3 (1.2) kg/m2] adults completed a 6-week SET intervention. Anthropometric and aerobic indicators as well the homeostasis model assessment index for insulin resistance index (HOMA-IR) were assessed at baseline and after SET. The low- and high-frequency [(LF (0.03–0.15 Hz) in ms2 and HF (0.15–0.4 Hz) in ms2)] analysis of HRV as well as adrenaline (A in nmol/l) and noradrenaline (NA in nmol/l) responses were assessed at resting condition and during ventilatory threshold 1 (VT1) of a graded maximal test at baseline and after SET. At baseline, resting HF, LF and the LF/HF ratio were different among groups (P < 0.01, respectively) and were significantly associated with waist-to-hip ratio (β = −0.26; p = 0.01, β = −0.12; p = 0.01 and, β = 0.21; p = 0.01). During exertion at VT1, only LF/HF ratio was associated with NA responses (β = 0.23; p = 0.01). After SET, the frequency domain marker improved significantly for both groups in comparison to baseline. These improvements are manifested by LF and HF increases and LF/HF ratio decreases in the rest condition (p < 0.01, respectively) and during exertion at VT1 (p < 0.01, respectively). The improvement in LH and HF were associated with VO2max increases (β = 0.22 p = 0.01 and β = 0.33; p = 0.01). The decreases observed for the LF/HF ratio are mainly associated to NA decreases observed at rest (β = 0.31; p = 0.001) and at VT1 (β = 0.38; p = 0.001). Obese adults have altered HRV, and 6 weeks of SET improves HRV variables at rest and during VT1 exertion. While LF and HF improvement were associated with VO2max increases, the LF/HF ratio was mainly associated with noradrenaline decreases observed at rest and at VT1.
Influence of Ethylene Signaling in the Crosstalk Between Fe, S, and P Deficiency Responses in Arabidopsis thaliana
Authors: García María José , Angulo Macarena , García Carlos , Lucena Carlos , Alcántara Esteban , Pérez-Vicente Rafael , Romera Francisco Javier
Published: March 2021

To cope with P, S, or Fe deficiency, dicot plants, likeArabidopsis, develop several responses (mainly in their roots) aimed to facilitate the mobilization and uptake of the deficient nutrient. Within these responses are the modification of root morphology, an increased number of transporters, augmented synthesis-release of nutrient solubilizing compounds and the enhancement of some enzymatic activities, like ferric reductase activity (FRA) or phosphatase activity (PA). Once a nutrient has been acquired in enough quantity, these responses should be switched off to minimize energy costs and toxicity. This implies that they are tightly regulated. Although the responses to each deficiency are induced in a rather specific manner, crosstalk between them is frequent and in such a way that P, S, or Fe deficiency can induce responses related to the other two nutrients. The regulation of the responses is not totally known but some hormones and signaling substances have been involved, either as activators [ethylene (ET), auxin, nitric oxide (NO)], or repressors [cytokinins (CKs)]. The plant hormone ET is involved in the regulation of responses to P, S, or Fe deficiency, and this could partly explain the crosstalk between them. In spite of these crosslinks, it can be hypothesized that, to confer the maximum specificity to the responses of each deficiency, ET should act in conjunction with other signals and/or through different transduction pathways. To study this latter possibility, several responses to P, S, or Fe deficiency have been studied in theArabidopiswild-type cultivar (WT) Columbia and in some of its ethylene signaling mutants (ctr1, ein2-1, ein3eil1) subjected to the three deficiencies. Results show that key elements of the ET transduction pathway, like CTR1, EIN2, and EIN3/EIL1, can play a role in the crosstalk among nutrient deficiency responses.
Mild Cognitive Impairment in de novo Parkinson's Disease: Selective Attention Deficit as Early Sign of Neurocognitive Decay
Authors: Cammisuli Davide Maria , Pagni Cristina , Palermo Giovanni , Frosini Daniela , Bonaccorsi Joyce , Radicchi Claudia , Cintoli Simona , Tommasini Luca , Tognoni Gloria , Ceravolo Roberto , Bonuccelli Ubaldo
Published: March 2021

Background: In the present study, we aimed to better investigate attention system profile of Parkinson's disease-Mild Cognitive Impairment (PD-MCI) patients and to determine if specific attentional deficits are associated with 123I-FP-CIT SPECT.

Methods: A total of 44 de novo drug-naïve PD patients [(27) with normal cognition (PD-NC) and 17 with MCI (PD-MCI)], 23 MCI patients and 23 individuals with subjective cognitive impairment (SCI) were recruited at the Clinical Neurology Unit of Santa Chiara hospital (Pisa University Medical School, Italy). They were assessed by a wide neuropsychological battery, including Visual Search Test (VST) measuring selective attention. Performances among groups were compared by non-parametric tests (i.e., Kruskal-Wallis and Mann-Whitney, Bonferroni corrected). Further, Spearman's rank correlations were performed to explore the association between neuropsychological variables and 123I-FP-CIT SPECT data in PD subgroup.

Results: PD-MCI patients performed worse on VST than patients with PD-NC (p = 0.002), patients with MCI and individuals with SCI (p < 0.001). The performance of PD-MCI patients on VST significantly correlated with caudate nucleus 123I-FP-CIT SPECT uptake (rho = 0.582, p < 0.05), whereas a negative correlation between such test and 123I-FP-CIT SPECT uptake in the left putamen (rho = −0.529, p < 0.05) was found in PD-NC patients.

Conclusions: We suggest that selective attention deficit might be a trigger of cognitive decay in de novo PD-MCI patients. The VST should be routinely used to detect attentional deficits in hospital clinical practice, in the light of its closely association with dopamine depletion of basal ganglia in mildly impaired PD patients.
Interpersonal Emotion Regulation: From Research to Group Therapy
Authors: Messina Irene , Calvo Vincenzo , Masaro Chiara , Ghedin Simona , Marogna Cristina
Published: March 2021

The concept of interpersonal emotion regulation (IER) refers to a variety of processes in which emotion regulation occurs as part of live social interactions and includes, among others, also those interpersonal interactions in which individuals turn to others to be helped or to help the others in managing emotions. Although IER may be a concept of interest in group therapy, specific theoretical insights in this field appear to be missed. In this article, we firstly provide a review of IER definitions, of classifications of IER strategies, and of IER clinical conceptualizations. Afterwards, we discuss the relevance of considering IER for group therapy, both in terms of non-specific group therapeutic factors and of group therapy techniques promoting adaptive emotion regulation, underlining the potentially relevant role of IER behaviors as intrinsically involved in group experience.
The Relationship Between Anxiety Levels, Sleep, and Physical Activity During COVID-19 Lockdown: An Exploratory Study
Authors: Frontini Roberta , Rebelo-Gonçalves Ricardo , Amaro Nuno , Salvador Rogério , Matos Rui , Morouço Pedro , Antunes Raul
Published: March 2021

Nowadays and worldwide, the attention is focused on coronavirus disease (COVID-19), and its consequences on mental health are yet to be fully understood. It is important to capture differences in anxiety levels among populations, groups, and the gender-related variation. Therefore, the present study had two main purposes: (1) to characterize the levels of state anxiety and trait anxiety by examining gender-related, sleep-related, and physical activity-related variations in a nonrepresentative sample of the Portuguese population during the first weeks of lockdown; and (2) to explore the possible relationship between trait anxiety and state anxiety and the possible role of gender as a moderator. This cross-sectional study comprised 1,332 Portuguese adults (aged 18–55 years old) recruited online during COVID-19 outbreak measures. Participants answered to sociodemographic data and the Portuguese version of the State-Trait Anxiety Inventory (STAI). Gender differences were found in both state anxiety (p = < 0.001; d = 0.385) and trait anxiety (p = < 0.001; d = 0.467) with females presenting higher values. People reporting doing more physical activity than usual during COVID-19 lockdown presented lower levels of state anxiety (p = < 0.001; d = 0.200). People reporting more satisfaction with the quality of sleep presented lower levels of both state anxiety (p = < 0.001; d = 0.701) and trait anxiety (p = < 0.001; d = 0.899). Variation associated with the physical activity level (low, moderate, and high) was significantly different among groups in both state anxiety (p = < 0.001) and trait anxiety (p = < 0.001). When analyzing in more detail separating the levels of physical activity, participants performing moderate and high physical activity showed lower values of state and trait anxiety compared to participants with low physical activity. Participants performing high physical activity also showed lower values of state anxiety compared to participants performing moderate physical activity. Higher levels of trait anxiety were related to higher levels of state anxiety, but this association was not moderated by gender. Interventions aiming to support people psychologically during this outbreak should consider anxiety as well as gender and possible behavioral changes in sleep and physical activity, for example. Health professionals should not only consider the anxiety related to the situation we are living but also address trait anxiety to help overcome COVID-19 psychological consequences.
Fine Classification of QSOs and Seyferts for Activity Types Based on SDSS Spectroscopy
Authors: Mickaelian A. M. , Abrahamyan H. V. , Paronyan G. M. , Mikayelyan G. A.
Published: March 2021

Using the SDSS spectroscopy, we have carried out fine optical spectral classification for activity types for 710 AGN candidates. These objects come from a larger sample of some 2,500 candidate AGN using pre-selection by various samples; bright objects of the Catalog of Quasars and Active Galactic Nuclei, AGN candidates among X-ray sources, optically variable radio sources, IRAS extragalactic objects, etc. A number of papers have been published with the results of this spectral classification. More than 800 QSOs have been identified and classified, including 710 QSOs, Seyferts and Composites. The fine classification shows that many QSOs show the same features as Seyferts, i.e., subtypes between S1 and S2 (S1.2, S1.5, S1.8 and S1.9). We have introduced subtypes for the QSOs: QSO1.2, QSO1.5, QSO1.8, QSO1.9, though the last subtype does not appear in SDSS wavelength range due to mostly highly redshifted Hα (the main line for identification of the 1.9 subtype). Thus, independent of the luminosity (which serves as a separator between QSOs and Seyferts), AGN show the same features. We also have classified many objects as Composites, spectra having composite characteristics between Sy and LINERs, Sy and HII or LINERs and HII; in some cases all three characteristics appear together resulting as Sy/LINER/HII subtype. The QSOs subtypes together with Seyfert ones allow to follow AGN properties along larger redshift range expanding our knowledge on the evolution of AGN to more distant Universe represented by QSOs.
Identification of DNA-Repair-Related Five-Gene Signature to Predict Prognosis in Patients with Esophageal Cancer
Authors: Wang Lin , Li Xueping , Zhao Lan , Jiang Longyang , Song Xinyue , Qi Aoshuang , Chen Ting , Ju Mingyi , Hu Baohui , Wei Minjie , He Miao , Zhao Lin
Published: March 2021

Esophageal cancer (ESCA) is a leading cause of cancer-related mortality, with poor prognosis worldwide. DNA damage repair is one of the hallmarks of cancer. Loss of genomic integrity owing to inactivation of DNA repair genes can increase the risk of cancer progression and lead to poor prognosis. We aimed to identify a novel gene signature related to DNA repair to predict the prognosis of ESCA patients. Based on gene expression profiles of ESCA patients from The Cancer Genome Atlas and gene set enrichment analysis, 102 genes related to DNA repair were identified as candidates. After stepwise Cox regression analysis, we established a five-gene prognostic model comprising DGCR8, POM121, TAF9, UPF3B, and BCAP31. Kaplan-Meier survival analysis confirmed a strong correlation between the prognostic model and survival. Moreover, we verified the clinical value of the prognostic signature under the influence of different clinical parameters. We found that small-molecule drugs (trametinib, selumetinib, and refametinib) could help to improve patient survival. In summary, our study provides a novel and promising prognostic signature based on DNA-repair-related genes to predict survival of patients with ESCA. Systematic data mining provides a theoretical basis for further exploring the molecular pathogenesis of ESCA and identifying therapeutic targets.
Recent Advances in the Molecular Design and Applications of Multispecific Biotherapeutics
Authors: Zhong Xiaotian , D’Antona Aaron M.
Published: March 2021

Recombinant protein-based biotherapeutics drugs have transformed clinical pipelines of the biopharmaceutical industry since the launch of recombinant insulin nearly four decades ago. These biologic drugs are structurally more complex than small molecules, and yet share a similar principle for rational drug discovery and development: That is to start with a pre-defined target and follow with the functional modulation with a therapeutic agent. Despite these tremendous successes, this “one target one drug” paradigm has been challenged by complex disease mechanisms that involve multiple pathways and demand new therapeutic routes. A rapidly evolving wave of multispecific biotherapeutics is coming into focus. These new therapeutic drugs are able to engage two or more protein targets via distinct binding interfaces with or without the chemical conjugation to large or small molecules. They possess the potential to not only address disease intricacy but also exploit new therapeutic mechanisms and assess undruggable targets for conventional monospecific biologics. This review focuses on the recent advances in molecular design and applications of major classes of multispecific biotherapeutics drugs, which include immune cells engagers, antibody-drug conjugates, multispecific tetherbodies, biologic matchmakers, and small-scaffold multispecific modalities. Challenges posed by the multispecific biotherapeutics drugs and their future outlooks are also discussed.
Does the Current State of Evidence Justify the Broad Use of Cross-Links in Dorsal Instrumentation? A Systematic Review
Authors: Heck Vincent J. , Oikonomidis Stavros , Prasse Tobias , Meyer Carolin , Scheyerer Max J. , Wetsch Wolfgang A. , Eysel Peer , Bredow Jan
Published: March 2021

Cross-links increase the stability of screw-rod systems in biomechanical testing. The aim of this systemic review was to find evidence pertaining to the additional benefit of the implantation of cross-links in clinical practice in regard to different spinal diseases. Therefore, a systematic literature analysis of two online databases was performed according to the PRISMA statement. Inclusion criteria were prospective and retrospective studies investigating the use of cross-links in dorsal instrumentation. Biomechanical studies and case series were excluded. A total of seven retrospective studies remained for final full-text evaluation. In total, two studies each address the use of cross-links in adolescent idiopathic scoliosis, neuromuscular scoliosis or atlantoaxial fusion, one study in congenital scoliosis. In atlantoaxial fusion the additional use of cross-links may provide earlier bony fusion. In surgical treatment for pediatric scoliosis the additional use of cross-links does not provide additional benefit. Radiological outcome and complication rate did not differ in between groups. No study addressed the use of cross-links in short- or long-segment fusion due to degenerative or traumatic disorders of the spine. There is a deficiency in published literature towards the impact of cross-links in spinal surgery. The current clinical evidence data do not confirm the biomechanical advantages of cross-links in clinical practice. Further studies are needed to warrant the use of cross-links in the future.
Chloropentaphenyldisiloxane—Model Study on Intermolecular Interactions in the Crystal Structure of a Monofunctionalized Disiloxane
Authors: Bauer Jonathan O. , Götz Tobias
Published: March 2021

Small functional siloxane units have gained great interest as molecular model systems for mimicking more complex silicate structures both in nature and in materials chemistry. The crystal structure of chloropentaphenyldisiloxane, which was synthesized for the first time, was elucidated by single-crystal X-ray diffraction analysis. The molecular crystal packing was studied in detail using state-of-the-art Hirshfeld surface analysis together with a two-dimensional fingerprint mapping of the intermolecular interactions. It was found that the phenyl C–H bonds act as donors for both weak C–H···π and C–H···Cl hydrogen bond interactions. The influence of intramolecular Si–O–Si bond parameters on the acceptor capability of functional groups in intermolecular hydrogen bond interactions is discussed.
Granger Causality on forward and Reversed Time Series
Authors: Chvosteková Martina , Jakubík Jozef , Krakovská Anna
Published: March 2021

In this study, the information flow time arrow is investigated for stochastic data defined by vector autoregressive models. The time series are analyzed forward and backward by different Granger causality detection methods. Besides the normal distribution, which is usually required for the validity of Granger causality analysis, several other distributions of predictive errors are considered. A clear effect of a change in the order of cause and effect on the time-reversed series of unidirectionally connected variables was detected with standard Granger causality test (GC), when the product of the connection strength and the ratio of the predictive errors of the driver and the recipient was below a certain level, otherwise bidirectional causal connection was detected. On the other hand, opposite causal link was detected unconditionally by the methods based on the time reversal testing, but they were not able to detect correct bidirectional connection. The usefulness of the backward analysis is manifested in cases where falsely detected unidirectional connections can be rejected by applying the result obtained after the time reversal, and in cases of uncorrelated causally independent variables, where the absence of a causal link detected by GC on the original series should be confirmed on the time-reversed series.
Impact of COVID19 on Operational Activities of Manufacturing Organizations—A Case Study and Industry 4.0-Based Survive-Stabilise-Sustainability (3S) Framework
Authors: Dar Mutahhar A. , Gladysz Bartlomiej , Buczacki Aleksander
Published: March 2021

The SARS-CoV-2 pandemic is spreading rapidly and threatening lives all over the globe. Due to the pandemic, economies all over the world are in deep distress due to disruption in work and operations across all sectors. The present case study was performed for a private Indian manufacturing company that has been affected to a great extent due to this pandemic, as India has the second highest number of SARS-CoV-2 cases after the USA. The deeply troubled company’s current predicament is very complex, as various factors are responsible for the crisis. In order to restore the company back to normal functioning, these factors were studied and evaluated. A diagnostic survey was conducted to obtain data about current working conditions of company. Data from the survey were analyzed qualitatively and quantitatively to identify and evaluate the disruptive factors. Based on this analysis, problems in operational activities were identified and appropriate improvement actions (best practices) were proposed. Furthermore, the proposed improvement actions were assessed from a sustainability perspective to check their feasibility. The best practices framework was generalized to a three-step continuous improvement framework oriented around preparedness for future pandemic-like disruptions. The proposed framework may constitute an indication and set of best practices for consideration by other manufacturing companies that find themselves in a similar situation to the analyzed case study.
Small-Scale Biodiesel Production Plants—An Overview
Authors: De Paola Maria Gabriela , Mazza Ivan , Paletta Rosy , Lopresto Catia Giovanna , Calabrò Vincenza
Published: March 2021

Small-scale plants that produce biodiesel have many social, economic and environmental advantages. Indeed, small plants significantly contribute to renewable energy production and rural development. Communities can use/reuse local raw materials and manage independently processes to obtain biofuels by essential, simple, flexible and cheap tools for self-supply. The review and understanding of recent plants of small biodiesel production is essential to identify limitations and critical units for improvement of the current process. Biodiesel production consists of four main stages, that are pre-treatment of oils, reaction, separation of products and biodiesel purification. Among lots of possibilities, waste cooking oils were chosen as cheap and green sources to produce biodiesel by base-catalyzed transesterification in a batch reactor. In this paper an overview on small-scale production plants is presented with the aim to put in evidence process, materials, control systems, energy consumption and economic parameters useful for the project and design of such scale of plants. Final considerations related to the use of biodiesel such as renewable energy storage (RES) in small communities are discussed too.
In Situ Evaluation of the U-Value of a Window Using the Infrared Method
Authors: Park Seyoung , Kim Seo Hoon , Jeong Hakgeun , Do Sung Lok , Kim Jonghun
Published: March 2021

The amount of heat lost through the envelope of a building is one of the most important variables that affects the energy performance evaluation of a house. In addition, it is especially important to estimate and accurately diagnose the amount of heat produced by windows. In Korea, windows’ U-values reflect a building’s initial design values and thermal characteristics that determine the thermal performance of an existing building, and is a factor that can overestimate the energy performance of a building. Therefore, there is a need for a field measurement method that can accurately measure the total U-value of windows in an existing house. This study provides a method of quantitatively measuring the total U-value of windows using the infrared (IR) method in ISO 9869-2. As a result of measuring the U-value using the infrared (IR) method, the Korean Standard (KS F 2278) for window performance test result values and the root mean square error (cvRMSE) for the U-value measurements using the IR method showed a high accuracy of about 3.29%. In addition, we confirmed that the IR method is an effective (cvRMSE about 7% improvement) method that can measure the comparison result faster than the heat flow meter (HFM) method, which is a conventional thermal performance measurement method.
Characterization of Gas Transport Properties of Compacted Solid Waste Materials
Authors: Iqbal Muhammad Rashid , Nandika Hiniduma Liyanage Damith , Isobe Yugo , Kawamoto Ken
Published: March 2021

Gas transport parameters such as gas diffusivity (Dp/D0), air permeability (ka), and their dependency on void space (air-filled porosity, ε) in a waste body govern convective air and gas diffusion at solid waste dumpsites and surface emission of various gases generated by microbial processes under aerobic and anaerobic decompositions. In this study, Dp/D0(ε) and ka(ε) were measured on dumping solid waste in Japan such as incinerated bottom ash and unburnable mixed waste as well as a buried waste sample (dumped for 20 years). Sieved samples with variable adjusted moistures were compacted by a standard proctor method and used for a series of laboratory tests for measuring compressibility, saturated hydraulic conductivity, and gas transport parameters. Results showed that incinerated bottom ash and unburnable mixed waste did not give the maximum dry density and optimum moisture content. Measured compressibility and saturated hydraulic conductivity of tested samples varied widely depending on the types of materials. Based on the previously proposed Dp/D0(ε) models, the diffusion-based tortuosity (T) was analyzed and unique power functional relations were found in T(ε) and could contribute to evaluating the gas diffusion process in the waste body compacted at different moisture conditions.
Profiling of Primary Metabolites and Volatile Determinants in Mahlab Cherry (Prunus mahaleb L.) Seeds in the Context of Its Different Varieties and Roasting as Analyzed Using Chemometric Tools
Authors: Farag Mohamed A. , Khattab Amira R. , Shamma Samir , Afifi Sherif M.
Published: March 2021

Mahlab cherry (Prunus mahaleb L.) is a plant native to the Mediterranean basin and Eastern Europe, with several health benefits and culinary uses. We explored the compositional heterogeneity in the aroma profile and nutrients of three P. mahaleb seeds in the context of its cultivar type, i.e., white and red, and in response to roasting. A holistic untargeted metabolomics approach was employed for the first time using solid-phase microextraction (SPME–GC–MS) profiles of seed volatiles and primary metabolites coupled with chemometrics. Around 65 peaks belonging to sugars, fatty acids, esters and organic acids were identified by GC–MS. White mahlab from Egypt is rich in fatty acids, e.g., oleic and α-linolenic acids. Some acyl esters, e.g., glycerylmonostearate and n-butylcaprylate, characterized mahlab cultivars from various origins. A total of 135 volatiles were identified, with organic acids and aldehydes the most abundant. Aldehydes were the most discriminatory in seed origin and in accounting for its distinct aroma. Several roasting indices were identified, viz. 1-octanol, γ-caprolactone and isomintlactone. A direct relationship between furans and fatty acids was rationalized by cyclic transformation of the latter into furan derivatives. This study provides the first chemical evidence supporting the nutritional and flavor determinants of mahlab seeds, suggesting novel uses as a functional food.
The Historical Trauma and Resilience of Individuals of Mexican Ancestry in the United States: A Scoping Literature Review and Emerging Conceptual Framework
Authors: Orozco-Figueroa Araceli
Published: March 2021

Recently, Black, Indigenous, and other People of Color (BIPOC) have encountered an escalation in adverse social conditions and trauma events in the United States. For individuals of Mexican ancestry in the United States (IMA-US), these recent events represent the latest chapter in their history of adversity: a history that can help us understand their social and health disparities. This paper utilized a scoping review to provide a historical and interdisciplinary perspective on discussions of mental health and substance use disorders relevant to IMA-US. The scoping review process yielded 16 peer reviewed sources from various disciplines, published from 1998 through 2018. Major themes included historically traumatic events, inter-generational responses to historical trauma, and vehicles of transmission of trauma narratives. Recommendations for healing from historical and contemporary oppression are discussed. This review expands the clinical baseline knowledge relevant to the diagnosis, treatment, and prevention of contemporary traumatic exposures for IMA-US.
An “Entirely Personal” Success: Intertextuality and Self-Reflexive Ironies in Henry James’s “Pandora”
Authors: Vellucci Sabrina
Published: March 2021

Henry James’s self-allusions in “Pandora” have been read as a rewriting of his former treatment of the “American Girl abroad” in the comic mode. The hints at “a Tauchnitz novel by an American author” (90) establish an ironical reversal of the failures of understanding which had led to tragedy in “Daisy Miller.” Yet the ironies in “Pandora” are multi-layered, often self-reflexive, and can be further interpreted in the light of James’s controversial adaptation of his famous novella for the stage. In this framework, well-known Jamesian topoi appear both as a (self-)parody and a metaliterary dialogue James engages with his readers and critics. The author’s personal implication in this “American” story is further testified by his Notebooks, in which James states his intention to write about his friends Henry and “Clover” Adams. Indeed, “Pandora”’s multi-layered intertextuality includes undeclared references to Adams’s anonymously published novel, Democracy, a semi-satirical account of U.S. political life. My article focuses on the web of intertextual relations woven in this short story with a view to reflecting on James’s ideas concerning the politics of authorship, readership, literary success, and the fate of the American Girl.
Telomeres in Interstitial Lung Disease
Authors: Stock Carmel J. W. , Renzoni Elisabetta A.
Published: March 2021

Interstitial lung diseases (ILD) encompass a group of conditions involving fibrosis and/or inflammation of the pulmonary parenchyma. Telomeres are repetitive DNA sequences at chromosome ends which protect against genome instability. At each cell division, telomeres shorten, but the telomerase complex partially counteracts progressive loss of telomeres by catalysing the synthesis of telomeric repeats. Once critical telomere shortening is reached, cell cycle arrest or apoptosis are triggered. Telomeres progressively shorten with age. A number of rare genetic mutations have been identified in genes encoding for components of the telomerase complex, including telomerase reverse transcriptase (TERT) and telomerase RNA component (TERC), in familial and, less frequently, in sporadic fibrotic ILDs. Defects in telomerase result in extremely short telomeres. More rapidly progressive disease is observed in fibrotic ILD patients with telomere gene mutations, regardless of underlying diagnosis. Associations with common single nucleotide polymorphisms in telomere related genes have also been demonstrated for various ILDs. Shorter peripheral blood telomere lengths compared to age-matched healthy individuals are found in a proportion of patients with fibrotic ILDs, and in idiopathic pulmonary fibrosis (IPF) and fibrotic hypersensitivity pneumonitis (HP) have been linked to worse survival, independently of disease severity. Greater susceptibility to immunosuppressant-induced side effects in patients with short telomeres has been described in patients with IPF and with fibrotic HP. Here, we discuss recent evidence for the involvement of telomere length and genetic variations in the development, progression, and treatment of fibrotic ILDs.
CMIP5-Based Projection of Decadal and Seasonal Sea Surface Temperature Variations in East China Shelf Seas
Authors: Lu Huiqiang , Xie Chuan , Zhang Cuicui , Zhai Jingsheng
Published: March 2021

The East China Shelf Seas, comprising the Bohai Sea, the Yellow Sea, and the shelf region of East China Sea, play significant roles among the shelf seas of the Western North Pacific Ocean. The projection of sea surface temperature (SST) changes in these regions is a hot research topic in marine science. However, this is a very difficult task due to the lack of available long-term projection data. Recently, with the high development of simulation technology based on numerical models, the model intercomparison projects, e.g., Phase 5 of the Climate Model Intercomparison Project (CMIP5), have become important ways of understanding climate changes. CMIP5 provides multiple models that can be used to estimate SST changes by 2100 under different representative concentration pathways (RCPs). This paper developed a CMIP5-based SST investigation framework for the projection of decadal and seasonal variation of SST in East China Shelf Seas by 2100. Since the simulation results of CMIP5 models may have degrees of errors, this paper uses hydrological observation data from World Ocean Atlas 2018 (WOA18) for model validation and correction. This paper selects seven representative ones including ACCESS1.3, CCSM4, FIO-ESM, CESM1-CAM5, CMCC-CMS, NorESM1-ME, and Max Planck Institute Earth System Model of medium resolution (MPI-ESM-MR). The decadal and seasonal SST changes in the next 100 years (2030, 2060, 2090) are investigated by comparing with the present analysis in 2010. The experimental results demonstrate that SST will increase significantly by 2100: the decadal SST will increase by about 1.55 °C, while the seasonal SST will increase by 1.03–1.95 °C.
The Use of Thermal Techniques in the Characterization of Bio-Sourced Polymers
Authors: Blanco Ignazio , Siracusa Valentina
Published: March 2021

The public pressure about the problems derived from the environmental issues increasingly pushes the research areas, of both industrial and academic sectors, to design material architectures with more and more foundations and reinforcements derived from renewable sources. In these efforts, researchers make extensive and profound use of thermal analysis. Among the different techniques available, thermal analysis offers, in addition to high accuracy in the measurement, smartness of execution, allowing to obtain with a very limited quantity of material precious information regarding the property–structure correlation, essential not only in the production process, but overall, in the design one. Thus, techniques such as differential scanning calorimetry (DSC), differential thermal analysis (DTA), dynamic mechanical analysis (DMA) and thermogravimetric analysis (TGA) were, are, and will be used in this transition from fossil feedstock to renewable ones, and in the development on new manufacturing processes such as those of additive manufacturing (AM). In this review, we report the state of the art of the last two years, as regards the use of thermal techniques in biopolymer design, polymer recycling, and the preparation of recyclable polymers as well as potential tools for biopolymer design in AM. For each study, we highlight how the most known thermal parameters, namely glass transition temperature (Tg), melting temperature (Tf), crystallization temperature (Tc) and percentage (%c), initial decomposition temperature (Ti), temperature at maximum mass loss rate (Tm), and tan δ, helped the researchers in understanding the characteristics of the investigated materials and the right way to the best design and preparation.
Conceptual Study and Manufacturing of a Configurable and Weld-Free Lattice Base for Automatic Food Machines
Authors: Pirondi Alessandro , Liberini Andrea , Rocchi Flavio
Published: March 2021

The study is aimed at developing a modular lattice base for automatic food machines, starting with a solution already patented by some of the authors. In this case, welded carpentry modules were interlocked with a system of profiles and metal inserts, also in welded carpentry, and the union was stabilized by structural adhesive bonding. Since welding involves long processing times and thermal distortions to be restored later, the driver of this study is to limit the use of welding as much as possible while increasing the modularity of the construction. For this purpose, various solution concepts have been generated where a common feature is the presence of rods of the same geometry and section to be joined together in configurable structural nodes. The concepts are qualitatively evaluated in light of the requirements, and the selected concept is digitally and physically prototyped. The prototype has been in service from over 5 years without showing any problems whatsoever.
Physical, Chemical, Mechanical, and Biological Properties of Four Different Commercial Root-End Filling Materials: A Comparative Study
Authors: Kang Tae-Yun , Choi Ji-Won , Seo Kyoung-Jin , Kim Kwang-Mahn , Kwon Jae-Sung
Published: March 2021

Commercial mineral trioxide aggregate (MTA) materials such as Endocem MTA (EC), Dia-Root Bio MTA (DR), RetroMTA (RM), and ProRoot MTA (PR) are increasingly used as root-end filling materials. The aim of this study was to assess and compare the physicochemical and mechanical properties and cytotoxicity of these MTAs. The film thicknesses of EC and DR were considerably less than that of PR; however, RM’s film thickness was greater than that of PR. In addition, the setting times of EC, DR, and RM were shorter than that of PR (p < 0.05). The solubility was not significantly different among all groups. The three relatively new MTA groups (EC, DR, and RM) exhibited a significant difference in pH variation and calcium ion release relative to the PR group (p < 0.05). The radiopacity of the three new MTAs was considerably less than that of PR. The mechanical strength of RM was not significantly different from that of PR (p > 0.05); however, the EC and DR groups were not as strong as PR (p < 0.05). All MTA groups revealed cytocompatibility. In conclusion, the results of this study confirmed that EC, RM, DR, and PR exhibit clinically acceptable physicochemical and mechanical properties and cell cytotoxicity.