bluuebunny/crossref_metadata_2025 huggingface dataset:
Workplace accommodation requests: Experiences of barriers and facilitators among deaf and hard-of-hearing
Authors: Dong Shengli , Meros Theodore , Seenath Sarika
Published: June 2023

BACKGROUND: Despite the protections afforded by the Americans with Disabilities Act (ADA), workers who are deaf and hard-of-hearing (DHH) face workplace challenges that may require the aid of workplace resources and accommodations. Limited research has been conducted to understand the process of accommodation requests among DHH workers. OBJECTIVE: This qualitative study examined barriers and facilitators to requesting accommodations among DHH workers. METHODS: Fifty-nine DHH workers, recruited from organizations serving DHH workers across U.S., participated in the study. Participants’ open-ended survey responses were analyzed and coded into themes representing barriers or facilitators to participants’ decisions to request or withhold requests for accommodations. RESULTS: Facilitators to accommodation request include an employee’s effective use of self-efficacy and self-advocacy; supportive work environments; and the presence of peers with disabilities. Barriers to accommodation requests include the anticipation of negative perception of stigmatization in the workplace; lack of knowledge related to accommodations and ADA; absence of workplace support; ineffectual workplace structure, policies, or procedures; concerns surrounding costs; and physical and access barriers in the workplace. CONCLUSION: To fulfill the workers’ rights as guaranteed by the ADA, and to best serve the interests of the workplace, strategies are highlighted on how to assist DHH employees to take advantage of workplace accommodations with aims to promote job retention and success.
Relationship between musculoskeletal discomfort and cell phone use among young adults: A cross-sectional survey
Authors: Al’Saani Syed Muhammad Ashraf Jahangeer , Raza Lubna , Fatima Khunsha , Khan Sehar , Fatima Mudebbera , Ali Syeda Nimrah , Amin Mehreen , Siddiqui Maheen , Liaquat Amna , Siddiqui Fatima , Naveed Wajeeha , Naqvi Tahira , Bibi Zainab
Published: June 2023

BACKGROUND: Cell phone usage is highly prevalent among young adults. They are used for multiple purposes including communication, studies, social networking and entertainment. However, its excessive usage has been associated with adverse health outcomes. OBJECTIVE: The objective was to find the association of cell phone usage with musculoskeletal discomfort (MSD) and its associated factors. METHODS: A cross-sectional study was conducted on young adult students from a low-middle income country over a period of 3 months from December 2018 to February 2019. A structured questionnaire based on the Disabilities of the Arm, Shoulder and Hand (QuickDASH scoring) was used to record the musculoskeletal discomfort. RESULTS: Out of 803 questionnaires, data of 754 (94%) were entered and the remaining questionnaires (n = 49) were discarded due to incomplete answers. The mean age was 20.83 (1.62) years. In our study, there were 194 (25.7%) males and 560 (74.3%) female participants. Neck and shoulder were the most frequently affected regions. A significant difference in QuickDASH score was observed between genders (p-value p ≤ 0.001). Left-handed individuals scored significantly higher on QuickDASH score compared to right-handed individuals (p < 0.05). Increased musculoskeletal discomfort was reported by individuals with daily cell phone use of more than four hours (p < 0.05). A positive significant correlation was found between continuous one hour cell-phone use and higher mean QuickDASH scores (correlation coefficient 0.124, p value 0.001). Shorter eye-to-screen distance was significantly associated with MSD (p < 0.05). CONCLUSION: Musculoskeletal discomfort is associated with the female gender, duration of cell phone use and a small eye-to-screen distance.
STOE Plenum Is Real
Authors: Hodge John
Published: June 2023

The “plenum” in the Scalar Theory of Everything (STOE) is the component of the universe that is a continuous medium that interacts with matter particles. Because a medium is not directly detected in instruments, the question of its reality is controversial. This is linked with the question of whether quantum waves are real waves in the plenum or merely probability waves. The experiment to demonstrate the reality of the plenum as a component of the universe has been done. The conclusion is that the plenum is a real component of the universe.
Issues of the Quality of the Forensic Expert Opinion and Some Forensic Errors
Authors:   National University of Defense of Ukraine, Ukraine, Kotlyarenko Lidiya , Myrovska Anna ,   National Academy of Internal Affairs Professor at the Department of Criminalistics, Ukraine, Pavlovska Nataliia ,   Kyiv National Economic University, Kiev, Ukraine, Patyk Lesia ,   National Academy of Internal Affairs, Ukraine, Zherebak Viktor ,   Zakarpattia region scientific research forensic center of the MIA (Ministry of Internal Affairs) of Ukraine, Ukraine
Published: June 2023

The concept of quality may be regarded as a composite structure comprising the support for the following elements: legal, scientific and technological, methodological, organisational, logistical. The quality of expert investigation is not an abstract concept; it actually exists and has a certain content that expresses substantial certainty, since it results in an expert opinion — judicial evidence. An expert opinion has a procedural (legal), scientific and technological aspects. The ‘quality of forensic examination’ concept therefore has a procedural (legal), scientific and technological content.
Origin, Emergence, Formation, Development, and Current State of Organized Crime in Ukraine
Authors:   Department of education, science and sports Ministry of Internal Affairs PhD in Law, Kiev, Ukraine, Kisiliuk Eduard , Kryshevych Olha ,   Department of Criminal Law of the National Academy of Internal Affairs, Kiev, Ukraine, Chabaniuk Vadim ,   Department of Criminal Law of the National Academy of Internal Affairs, Kyiv, Ukraine, Kulyk Maryna ,   National Academy of Internal Affairs, Kyiv, Ukraine, Symchuk Anatolii ,   National Academy Internal Affairs, Kyiv, Ukraine
Published: June 2023

The Criminal Code (hereinafter CC) of the Ukrainian SSR (§2, Art.41) only once provided for a crime committed by an organized group as an aggravating circumstance in any crime till 1989 [1]. At the close of 80-s and in 90-s especially the country faced a fast-growing and serious challenge from organized crime that caused a classifying circumstance - a commission by an organized criminal group (hereinafter OCG) amended in 20 articles, the Special Part, CC. Regulations, Session of the Supreme Court and Methodical Instructions, the Ministry of Interior did not give an appropriate definition of OCG. As a result law enforcement and, first of all, the Ministry of Interior agencies did not have strict legislative acts to define OCG from traditional groups of criminals. All the cited above could not but adversely affect the struggle against organized crime. According to the statistical data for 1991-2023, annual Reports on combating organized criminal groups, the Ministry of Interior the status of organized crime is as follows.
Preliminary Phytochemical Screening, Quantification of Bioactive Compounds and Antioxidant Potential of Achyranthes aspera L. Leaf Powder
Authors:   Department of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India., Harikatha Poornima , Devi Kodidhela Lakshmi ,   Department of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India., J. Chavan Jaykumar ,   Department of Botany, Yashavantrao Chavan Institute of Science, Satara, India.
Published: June 2023

Plant extracts have been used since ages for the treatment of various ailments in India and their market is growing globally as well. Achyranthes aspera is one such promising plant with known medicinal properties. Qualitative and quantitative phytochemical screening is necessary to evaluate the therapeutic and antioxidant properties of the medicinal plants. In the present study, we evaluated the bioactive chemical constituents and their in vitro antioxidant potential of A. aspera leaf extract in different solvents. The soxhlets of A. aspera leaf powder in different solvents – methanol, ethanol, aqueous and chloroform on phytochemical screening showed positive results for carbohydrates, phenols, tannins, flavonoids, saponins, cardiac glycosides and steroids. Alkaloids and terpenoids are present in all the extracts except in chloroform extract. Total contents phenols (22.282 gm/100 gm) were found higher as compared to total contents of flavonoids, saponins, alkaloids. Moreover, all antioxidant assays confirmed the antioxidant potential of leaves of A. aspera. Thus, A. aspera is rich in phytochemicals that are needed for its antioxidant potential against various disorders.
Effects of Urea Coated Hydroxyapatite Nanoparticles on Enzymatic and Nonenzymatic Antioxidants on Varieties of Brassica juncea L.
Authors:   School of Life Sciences, Jaipur National University, Jaipur, Rajasthan, India., Walia Alka , Sanadhya Dheera ,   School of Life Sciences, Jaipur National University, Jaipur, Rajasthan, India.
Published: June 2023

In the present investigation, urea coated hydroxyapatite nanoparticles (UHANPs) were synthesized, and characterized by using X-ray diffraction, FESEM, and FTIR techniques. Then, their effects at different concentrations (250 ppm, 500 ppm, 750 ppm, 1000 ppm) were observed for various enzymatic and non-enzymatic antioxidants of two varieties of Brassica juncea L. i.e. GIRIRAJ, and NRCBH- 101. Those were also weighup with control plants and plants grown in treatments with the same concentrations of urea. Results revealed that synthesized nanoparticles were 23-32 nm in size, and for all the parameters, the antioxidants like NPT, Polychelatins were found to the higher at treatment with 1000 ppm of Urea when compared to others while minimum concentration of these with UHANP (500 ppm). However, SOD increases with higher concentration of UHANPs and urea while other enzymatic antioxidants like APX and catalase declines. However, SOD declined with higher concentration of urea. All the results were statistically compared.
Citrus Fruit Peels: From Waste to Worth
Authors:   P. G. Department of Home Science, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India., Parmar Nidhi , Patel V. H. ,   P. G. Department of Home Science, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India., Roghelia Viraj ,   P. G. Department of Home Science, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India.
Published: June 2023

Citrus fruits are most commonly consumed fruits. However, the peels of these fruits yield a huge waste. The peels also contain important phytoconstituents. This study aimed to investigate the antioxidant properties of peels of two selected citrus fruits (sweet lime and lemon). The peels were extracted using four solvents namely methanol, ethanol, acetone and distilled water. The extracts were analysed for total phenolic content, flavonoid content, DPPH radical scavenging activity and ferric ion reducing antioxidant power (FRAP). The result revealed that highest antioxidant profile was found for methanol extract of both sweet lime peel and lemon peel as compared to other extracts. The extracts of peels of sweet lime possessed higher total phenol, flavonoid and FRAP. The antioxidant capacity in terms of DPPH RSA and FRAP were attributed mainly to total phenolics as compared to flavonoids for both the peels. The higher antioxidant potential of peels of selected citrus fruits make them key ingredient for the development of functional foods and other supplementary products.
Phytotoxic Effect of Heavy Metals on Growth Parameters of Mung Bean Seeds (Vigna radiata)
Authors:   Associate Professor, P.G. Department of Botany, Bareilly College, Bareilly, Uttar Pradesh, India., Rani Asha
Published: June 2023

In this investigation, effect of heavy metals present in city waste water on mung bean seeds was studied.The effluent significantly inhibited the germination, shoot and root length. The increase concentration of different heavy metals present in city waste water reduces the germination percentage, shoot length and root length of the seedlings. Reduction in all these parameters affects the other observed biochemical parameters i.e. chlorophyll content and nitrogen content.
Active Peacebuilding as a Challenging Task of the Catholic Social Ethics
Authors: Szaniszló Inocent-Mária V.
Published: June 2023

The God's Fifth Commandment is one of those commandments that are negatively formulated. Its wording is unambiguous, and acclaims: Thou shalt not kill! Yet, the history of humankind on our Blue Planet is rather marked with breaks in military conflicts than longer periods of peace. Regardless of a high likelihood of constantly recurring military conflicts, and despite an intense involvement of Christian culture of Europe in lots of military solutions of such conflicts in the course of history, Christian theology has always been opposed to killing as a way of resolving problems. At the most, the Church admitted that in certain instances of conflict resolution killing cannot be avoided, and it frequently referred to a just or defensive war, and developed its theological justification. In the society of that time, it was not easy to take a completely negative position on killing as advocated by many pacifists, or a number of Christianity-oriented sects (such as Quakers in Pennsylvania in the U.S.A., and the like). Still, only since the Second Vatican Council there has been discussion on active peacebuilding and preconditions under which it could work.
RESOLUSI KONFLIK ZONA EKONOMI EKSLUSIF INDONESIA DAN VIETNAM DALAM PENGAMANAN SUMBER DAYA MARITIM NATUNA UTARA
Authors: Yanto Andri
Published: June 2023

North Natuna is one part of the territorial waters of Indonesia which is very rich in resources. According to the applicable jurisdiction based on Law Number 17 of 1985 concerning the 1982 UNCLOS Ratification, North Natuna which has been proven to have the largest natural gas reserves in the Asia Pacific, is an inseparable part of Indonesia's EEZ. However, the 1982 ratification of UNCLOS in another country, namely Vietnam, also provides a similar explanation, that geographically North Natuna is its territory. This difference in conception has resulted in many incidents, from confrontations between law enforcement officers from the two countries, the capture of Vietnamese fishermen and the sinking of ships by the Indonesian authorities, to the complexity of the negotiation process between the two countries. In the midst of these problems, the governments of the two countries must make quick efforts in the face of growing external pressure, namely China's Nine Dash Line claims, as well as the need to use North Natuna itself. Using a juridical-normative research pattern, this paper attempts to unravel the 1982 UNCLOS provisions in conflict resolution and their relevance to Indonesia's strategy in resolving the EEZ dispute case with Vietnam in North Natuna.
Peran Perwakilan Diplomatik dalam Perlindungan terhadap Pekerja Migran Indonesia di Luar Negeri dalam Perspektif Hukum Internasional
Authors: Winda Nur Khotimah
Published: June 2023

Ketika warga negara berada dalam bahaya, negara harus turun tangan untuk memastikan keselamatan mereka di dalam dan luar negeri. Sudah bukan berita yang mengejutkan lagi bahwa Indonesia memiliki populasi pekerja asing yang cukup besar dari Indonesia, sehingga saat ini perlindungan terhadap mereka masih perlu dioptimalisasikan terkait pelaksanaannya. Kehadiran instrumen-instrumen hukum belum diimplementasikan sebagaimana yang tertulis. Perbedaan  nilai-nilai antara negara Indonesia dengan yang lain bisa menjadi hambatan dalam penanganan konflik yang dihadapi pekerja migran Indonesia. Dalam mengatasi konflik antarnegara, maka diperlukan untuk berpedoman pada sumber-sumber hukum internasional dan tidak hanya berpedoman pada salah satu hukum nasional suatu negara yang berkonflik. Penyelesaian konflik internasional umumnya dilakukan oleh perwakilan diplomatik yang ditugaskan ke suatu negara. Kajian mengenai perwakilan diplomatik ada dalam Hukum Diplomatik yang juga termasuk ke dalam kajian Hukum Internasional. Penyelesaian konflik internasional umumnya dilakukan oleh perwakilan diplomatik yang ditugaskan ke suatu negara. Kajian mengenai perwakilan diplomatik ada dalam Hukum Diplomatik yang juga termasuk ke dalam kajian Hukum Internasional.
Analisis Hukum Praktik Human Trafficking Dengan Modus Pengangkatan Anak Tanpa Penetapan Pengadilan
Authors: Harefa Farid Arby , Kalo Syafruddin , Marlina Marlina , Bariah Chairul
Published: June 2023

Procedurally, the applicable law states that a child adoption needs to go through the procedures for applying for a determination of adoption to the local District Court. If a court decision has not been obtained, it turns out that it can be reported to the local police. Children who are traded by their biological parents to other people, of course, involve various parties. For this reason, it is necessary to know the criminal responsibility of these perpetrators, and what is the basis for the panel of judges considerations in imposing a sentence on the defendant in a case of adoption which results in child trafficking as referred to in the P.N.Sim Decision. No. 398/Pid.Sus/2017 An. Defendant "G.M". The issues raised are: First, regulation of human trafficking crimes in Indonesia; and second, legal analysis of the practice of criminal acts of human trafficking with the mode of adoption without a court decision. 398/Pid.Sus/2017. This research is juridical-normative research which is descriptive-analytic. The results of this analysis show that: First, the provisions on criminal sanctions in the PTPPO Law cannot be used to indict perpetrators of child adoption without a court order; Second, based on the criminal responsibility of the Defendant "GM", he can be responsible for the mistakes he has made. It is recommended that the Government of Indonesia make a policy by summarizing the procedures for adopting children through the courts.
Pertanggungjawaban Ayah Terhadap Anak Kandung Sebagai Kewajiban Hukum Dalam Lingkup Rumah Tangga Berdasarkan Undang-Undang No. 23 Tahun 2004 Tentang Penghapusan Kekerasan Dalam Rumah Tangga
Authors: Sagala Rumanty Fitriana , Ablisar Madiasa , Siregar Mahmul , Nasution Mirza
Published: June 2023

Every husband/father in the household is prohibited from abandoning people within the scope of his family as stipulated in Article 9 paragraph (1) of the Law on the Elimination of Domestic Violence. Husband/father or because of approval or agreement, providing life, care, or maintenance to these biological children is obligatory. In positive law, a father is obliged to provide maintenance for his child. The issues raised are regarding 1) The responsibility of a father towards his biological children in terms of positive law; and 2) The legal considerations of the panel of judges regarding the responsibility of fathers towards their biological children; case studies of court decisions regarding child neglect. This research is juridical-normative research that is descriptive-analytical with a statutory approach. The study uses secondary data from the Medan District Court Decision collected by field study techniques at the Medan District Court Registrar's Office. Data analysis used qualitative data analysis methods. Results of the study: From the point of view of marriage law, the limits on the obligations and responsibilities of parents (including fathers) towards their biological children can be seen in Article 45 paragraph (2) of the Marriage Law. In implementing sentencing objectives, not all judges can explore the actual legal facts in a case. It is recommended that law enforcement officials in handling cases of criminal acts of child protection and domestic violence prioritize the best interests of the child.
Too simple, too complex, or just right? Advantages, challenges and resolutions for indicators of genetic diversity
Authors: Hoban Sean , da Silva Jessica , Hughes Alice , Hunter Maggie , Kalamujić Stroil Belma , Laikre Linda , Mastretta Yanes Alicia , Millette Katie , Paz-Vinas Ivan , Ruiz Luis , Shaw Robyn , Vernesi Cristiano
Published: June 2023

Measuring genetic diversity of wild species using DNA-based data remains resource intensive and time-consuming for nearly all species. Yet, genetic assessments are needed for global conservation commitments including the Convention on Biological Diversity and for governments and managers to evaluate conservation progress and to prioritize species and populations to preserve and recover genetic diversity (e.g., genetic rescue). Recently, indicators were developed for tracking and reporting genetic diversity status and trends for hundreds of species. The indicators quantify two simple proxies of within-population and among-population genetic diversity and adaptive potential: small effective size (Ne<500) and loss of genetically distinct populations. Indicators must balance scientific credibility, practicality, and simplicity. Here, we summarize the advantages of these pragmatic indicators; address critiques by scientists for simplifying assumptions and by policymakers for complexity; and propose solutions and next steps. We aim to support practitioners putting indicators into policy, action, legislation and reporting.
KUALITAS PELAYANAN PASPOR DI KANTOR IMIGRASI KELAS I TEMPAT PEMERIKSAAN IMIGRASI (TPI) SURAKARTA TAHUN 2022
Authors: Joenoes Andhika Rahmayana , Pramono Joko , Haryanto Aris Tri
Published: June 2023

The passport application process is easier, faster, with minimal data errors, andfriendly for the elderly and disabled at the Surakarta Immigration Office.However, in its implementation there are problems, including: 1) Not yet optimalinnovation of information technology-based public services. 2) Minimum numberof competent human resources in the field of information technology. 3) Thefacilities and infrastructure for supporting information technology-based servicesare not yet optimal. The purpose of this study was to analyze and determine thequality of passport services at the Class I Immigration Office at the SurakartaImmigration Checkpoint. This study uses a descriptive method with a qualitativeapproach. Research informants using purposive sampling technique. Datacollection techniques using interviews, and documentation. The data analysistechnique used is interactive analysis which consists of data reduction, datapresentation and conclusion drawing. The results showed that the service qualityof Surakarta immigration office employees from the dimension of tangibles hasnot fully provided satisfaction for community, including not optimal facilities andinfrastructure supporting information technology-based services and lack ofparking lots. From the dimension of reliability has not been seen well because ofthe minimum number of competent human resources in the field of InformationTechnology. In terms of responsiveness, has not shown optimal service quality.The dimension of assurance, shows the services that have been carried out inaccordance with the applicable provisions, but in terms of providing guarantees,the time for granting passports is sometimes not on time. From the dimension ofempathy shows a good quality of Service. In performing their duties, employeesserve well to passport applicants.Keywords: Tangibles, Reliability, Responsiveness, Assurance and Empathy
Together but Separate: Relationships and Boundaries between North and
South Koreans in Multiethnic Britain
Authors: Kim Jihye
Published: June 2023

The United Kingdom hosts the largest North Korean immigrant community in Europe, and the majority have settled in New Malden, London’s Koreatown. Drawing on ethnographic fieldwork, this study examines the relationships North Korean immigrants have established with their South Korean counterparts in the course of secondary migration from South Korea to the UK, focusing on how the shift from a majority–minority relationship between the two communities in ethnic-hierarchical South Korea to a minority–minority relationship in multi-ethnic Britain has influenced the North Koreans’ perceptions of and boundaries with South Koreans. Faced with language barriers and many other disadvantages in the host society, North Korean immigrants in the UK make pragmatic use of commonalities they share with their South Korean counterparts as well as of previous experiences and skills learned in South Korea. However, instead
of fully assimilating into the South Korean immigrant community, they freely
acknowledge the differences in the post-partition era, resulting in an enduring invisible boundary between the two groups. In doing so, they perceive that the two groups are in a relatively equal position as respective minorities vis-à-vis broader society, and the sense of ethnic stratification and hierarchy between them is largely dissolved. This study thus offers insights into how ethnic relations are contingent on social contexts and how migrants as transnational agents use and navigate their experiences, resources, and relationships to position themselves in the host society and shape their everyday life practices in a complex migration context.
Overseas Korean Adoption and the
Birth of Swedish Color-blindness: A Study of How Korean Adoptees Transformed Sweden’s Attitude to Race and the Relationship between Race and Swedishness
Authors: Hübinette Tobias
Published: June 2023

This article consists of a study of how the first wave of Korean adoptees to Sweden were imagined and represented in a political debate that raged throughout the 1960s concerning whether or not Swedes would adopt non-white children from abroad. The study examines how the arrival of the Korean adoptees came to transform Swedes’ attitude to race and the relationship between race and Swedishness, and how they made it possible for Sweden to transition from a country with a racial obsession to an antiracist nation in the 1960s, whenSweden was one of the Western world’s whitest and most racially homogeneous countries.
Testing the Effectiveness of Ethanol Extract of Horse Whip Leaves (Stachytarpheta Jamaicensis L.) towards Wounds in Mice (Mus musculus)
Authors: Ratulangi Wulan Ratia , Zohroyani  , Purmafitriah En , Baiq Ayu Aprilia Mustariani
Published: June 2023

The horse whip leaves plant (Stachytarpheta jamaincensis) is an annual herbaceous shrub. Cuts are wounds that occur due to cuts by instruments or sharps objects. This study aims to determine the effectiveness of the ethanol extract of horse whip leaves on the healing of cuts in mice (Mus musculus) and the most effective concentration for healing cuts in mice. Design This study uses laboratory experimental methods. The sample used for each experimental group was 5 mice with a total of 5 groups, so this study used 25 mice with 3 treatments, namely the treatment (positive control (+), negative control (-), 25%, 50%, 75%). All data is tabulated and statistically analyzed with ANOVA. The conclusion shows that the concentration of horsetail leaf extract at a concentration of 75% is the most effective in healing cuts in mice when compared to horsetail leaf extract at concentrations of 50% and 25% in terms of the average percentage of healing from day 1 to day 7.
SOSIALISASI PENERAPAN PERILAKU HIDUP BERSIH DAN SEHAT PADA MASYARAKAT DESA LANTAN, NUSA TENGGARA BARAT
Authors: Hijriani Baiq Isti Hijriani , Ajeng Dian Pertiwi  , Adriyan Suhada
Published: June 2023

Menciptakan hidup yang sehat sangatlah mudah dan sebaiknya diterapkan oleh setiap orang. Manfaat hidup sehat yang dapat diperoleh seperti meningkatkan kesehatan, konsentrasi kerja, sampai dengan keharmonisan dalam keluarga dan bermasyarakat. Salah satu langkah yang dapat dilakukan untuk mencapai derajat kesehatan yang optimal adalah dengan perilaku hidup bersih dan sehat (PHBS). Akan tetapi, kesadaran masyarakat di desa akan kesehatan dan pola hidup bersih sehat masih sangat rendah. Untuk itu tujuan pengabdian terkait perilaku hidup bersih sehat, diharapkan dapat menjadi upaya dalam menyadarkan masyarakat akan pentingnya melakukan upaya perilaku hidup bersih sehat dalam kehidupan sehari-hari sekaligus memberikan pengetahuan bagaimana cara merealisasikannya sehingga bisa terwujud masyarakat yang peduli sehat. Lokasi kegiatan pengabdian ini berada di Desa Lantan, Kecamatan Batukliang Utara, Kabupaten Lombok Tengah. Kegiatan sosialisasi tentang PHBS kepada masyarakat telah dilaksanakan dengan metode ceramah, diskusi dan tanya jawab. Secara umum, kegiatan pengabdian ini berjalan dengan lancar. Berdasarkan hal tersebut, kesimpulan yang dapat ditarik adalah bahwa peserta kegiatan pengabdian mengetahui bahwa perilaku hidup bersih dan sehat sangat penting untuk mencegah berbagai penyakit, salah satunya yaitu masyarakat sudah mengerti 6 langkah mencuci tangan khususnya dalam menghadapi pandemi Covid-19.
PEMANFAATAN SAMPAH ORGANIK MENJADI PUPUK UNTUK WARGA DESA PERAMPUAN
Authors: Asri Roushandy
Published: June 2023

Sampah merupakan salah satu permasalahan yang ada di Indonesia. Masyarakat masih memiliki pola pikir untuk membuang sampah di badan air atau membakar sampah. Hal ini akan berdampak menimbulkan penyakit dan ditiru oleh generasi muda. Sehingga perlu dilakukan pengolahan sampah agar dapat bernilai ekonomis. Salah satunya adalah pengolahan sampah organik menjadi pupuk. Oleh karena itu, dilakukan pelatihan pembuatan pupuk dari sampah organik yang berlokasi di Desa Perampuan, Kecamatan Labuapi, Kabupaten Lombok Barat. Peserta pelatihan adalah warga desa yang berjumlah 24orang. Kegiatan ini di awali dengan pemberian pre-test sebelum penyampaian materi dan demontrasi pembuatan pupuk. Selanjutnya ditutup dengan post-test untuk mengukur peningkatan pengetahuan peserta. Rata-rata pengetahuan ketika pre-test adalah di bawah 50% dan hasil analisis setelah post-test adalah di atas 90%. Jadi, dapat disimpulkan bahwa pengetahuan peserta meningkat secara signifikan.
OPTIMALISASI PENGGUNAAN MICROSOFT WORD DAN EXCEL UNTUK PEMBENAHAN DATA DI DESA TERONG TAWAH
Authors: Permata Reny Amalia , Suhada Adriyan , Pertiwi Ajeng Dian , Salleh Muhammad Asyraf bin , Munji Munji
Published: June 2023

Kegiatan administrasi perkantoran di Desa Terong Tawah belum sepenuhnya terkomputerisasi, banyak hal yang masih dilakukan secara manual. Oleh karena itu, tujuan pengabdian masyarakat ini adalah untuk meningkatkan kemampuan perangkat desa dan masyarakat sekitar agar mengoptimalkan penggunaan Microsfost Word dan Excel dalam  pembenahan data di Desa Terong Tawah. Sasaran kegiatan pengabdian ini adalah perangkat desa terong tawah dan masyarakat sekitar yang berjumlah 20 orang. Metode yang digunakan dalam pengabdian ini ada dua tahap, yakni tahapan persiapan untuk mengidentifikasi permasalahan apa yang dihadapi oleh peserta dengan memberikan pre-test. Kemudian tahapan berikutnya adalah tahap pelaksanaan, yaitu tahap pemberian materi kepada peserta, dengan ceramah, tanya jawab dan praktek langsung serta pemberian post-test pada sesi akhir. Setelah kegiatan pengabdian diperoleh peningkatan total sebesar 20.5% (menguasai dan sangat menguasai) kemampuan penggunaan Microsoft Word dan Excel berdasarkan hasil pre-test (50.5%) dan post-test (71%). Oleh karena itu, pengabdian masyarakat ini memberikan hasil berupa peningkatan kemampuan peserta sehingga dapat mengoptimalkan penggunaan Microsoft Word dan Excel untuk pembenahan data di Desa Terong Tawah.
Penyuluhan Peningkatan Pemahaman dan Pencegahan HIV/AIDS Bagi Masyarakat di kawasan wisata Desa Giri Sasak Kecamatan Kuripan Lombok Barat
Authors: Ozan Pauzan , Idham Halid
Published: June 2023

HIV adalah virus yang menyerang atau menginfeksi sistem kekebalan tubuh yang menyebabkan penurunan kekebalan tubuh manusia dan AIDS adalah kumpulan gejala yang disebabkan oleh virus HIV. HIV secara langsung menyerang dan menghancurkan sel CD4. Metode yang digunakan dalam kegiatan pengabdian masyarakat ini berupa sosialisasi kepada masyarakat tentang peningkatan pemahaman dan pencegahan penularan virus HIV/AIDS. Tujuan dari pengabdian masyarakat ini adalah untuk meningkatkan pengetahuan dan pemahaman masyarakat tentang pencegahan penularan dan akibat dari HIV/AIDS. Lokasi kegiatan di Desa Giri Sasak, Kecamatan Kuripan, Lombok Barat. Secara kumulatif dapat disimpulkan bahwa kegiatan edukasi HIV/AIDS untuk meningkatkan pengetahuan tentang pencegahan penularan dan akibat virus HIV, telah menunjukkan peningkatan pengetahuan dan pemahaman masyarakat peserta.
The effects of artificial weathering on the pendulum hardness of chestnut wood applied with polyurethane varnish after heat treatment
Authors: ULAY Göksel , AYATA Ümit
Published: June 2023

The effects of artificial weathering (576 h) on the pendulum hardness values of Anatolian chestnut (Castanea sativa Mill.) wood applied with polyurethane varnish (PU) after heat treatment at 190℃ for 1.5 h and at 212℃ for 2 h were investigated. The results revealed that heat treatment, weathering period, and interaction were significant. The hardness values of the heat-treated materials were higher than those of the non-heat-treated test samples. The highest hardness value was determined in the un-weathered group of the test samples that were heat-treated at 190°C for 1.5 h while the lowest value was determined in the group that was heat-treated at 190°C for 1.5 h and weathered for 432 h. The highest reduction rates after weathering were obtained on samples that were heat-treated at 190℃ for 1.5 h, followed by heat-treated at 212℃ for 2 h and non-heat-treated. It was observed that the heat treatment application caused different pendulum hardness values in chestnut wood samples covered with polyurethane varnish.
Cajanus cajan: a promissory high-nitrogen fixing cover crop for Uruguay
Authors: Berriel Verónica , Perdomo Carlos H.
Published: June 2023

Cover crops can increase agricultural sustainability by protecting soil from erosion, increasing biodiversity, and symbiotically incorporating fixed nitrogen (N) into the soil. Nowadays, however, in Uruguay mostly grasses are planted in autumn to protect the soil from erosion. Another option is to study tropical legumes’ performance as cover crops, which can fix substantial amounts of nitrogen in short growing periods, thereby bridging the knowledge gap in Uruguayan agriculture. The main objective was to evaluate and compare the performance of six tropical legumes (Crotalaria juncea, Crotalaria spectabilis, Crotalaria ochroleuca, Cajanus cajan, Dolichos lablab, Mucuna pruriens) and the temperate legume Glycine max. The evaluation focused on aboveground biomass and the N mass derived from fixation (NmdFix), as well as other attributes; three field experiments were conducted on a southern Uruguay farm during the summers of 2017, 2018, and 2019. The growing cycle lengths for the cover crops in 2017, 2018, and 2019 were 117, 130, and 90 days, respectively. The results showed that when planting was done at late December (2017 and 2018 growing cycles), the species with the highest mean biomass yield were Crotalaria juncea (two year average 12.0 Mg ha-1) and Cajanus cajan (11.0 Mg ha-1), but Cajanus cajan (149 kg ha-1) more than doubled the NmdFix mass of Crotalaria juncea (57 kg ha-1). In 2018 biomass yields were much higher than in 2017, with Glycine max (20.0 Mg ha-1) yielding at a similar level to Crotalaria juncea and Cajanus cajan (20.5 and 18.7 Mg ha-1, respectively). Amounts of NmdFix, however, were much higher in Glycine max and Cajanus cajan (263 and 253 kg N ha-1, respectively), than in Crotalaria juncea (91 kg N ha-1). In 2019 planting had to be delayed until early February and only Glycine max maintained acceptable biomass and NmdFix levels. In conclusion, based on its fixing N potential, for late December sowings Cajanus cajan and Glycine max would be the most promising species for cover crop use, while for late January or early February sowings, only Glycine max would an option because the tropical species seriously impaired their productivity when grew longer into the cooler autumn temperatures.
Human epidermal keratinocytes and human dermal fibroblasts interactions seeded on gelatin hydrogel for future application in skin in vitro 3-dimensional model
Authors: Tahri Safa , Maarof Manira , Masri Syafira , Che Man Rohaina , Masmoudi Hatem , Fauzi Mh Busra
Published: June 2023

Introduction: Plenty of biomaterials have been studied for their application in skin tissue engineering. Currently, gelatin-hydrogel is used to support three-dimensional (3D) skin in vitro models. However, mimicking the human body conditions and properties remains a challenge and gelatin-hydrogels have low mechanical properties and undergo rapid degradation rendering them not suitable for 3D in vitro cell culture. Nevertheless, changing the concentration of hydrogels could overcome this issue. Thus, we aim to investigate the potential of gelatin hydrogel with different concentrations crosslinked with genipin to promote human epidermal keratinocytes and human dermal fibroblasts culture to develop a 3D-in vitro skin model replacing animal models.

Methods: Briefly, the composite gelatin hydrogels were fabricated using different concentrations as follows 3%, 5%, 8%, and 10% crosslinked with 0.1% genipin or non-crosslinked. Both physical and chemical properties were evaluated.

Results and discussion: The crosslinked scaffolds showed better properties, including porosity and hydrophilicity, and genipin was found to enhance the physical properties. Furthermore, no alteration was prominent in both formulations of CL_GEL 5% and CL_GEL8% after genipin modification. The biocompatibility assays showed that all groups promoted cell attachment, cell viability, and cell migration except for the CL_GEL10% group. The CL_GEL5% and CL_GEL8% groups were selected to develop a bi-layer 3D-in vitro skin model. The immunohistochemistry (IHC) and hematoxylin and eosin staining (H&E) were performed on day 7, 14, and 21 to evaluate the reepithelization of the skin constructs. However, despite satisfactory biocompatibility properties, neither of the selected formulations, CL_GEL 5% and CL_GEL 8%, proved adequate for creating a bi-layer 3D in-vitro skin model. While this study provides valuable insights into the potential of gelatin hydrogels, further research is needed to address the challenges associated with their use in developing 3D skin models for testing and biomedical applications.
Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum
Authors: Poulalier-Delavelle Margaux , Baker Jonathan P. , Millard James , Winzer Klaus , Minton Nigel P.
Published: June 2023

Acetogenic bacteria can play a major role in achieving Net Zero through their ability to convert CO2 into industrially relevant chemicals and fuels. Full exploitation of this potential will be reliant on effective metabolic engineering tools, such as those based on the Streptococcus pyogenes CRISPR/Cas9 system. However, attempts to introduce cas9-containing vectors into Acetobacterium woodii were unsuccessful, most likely as a consequence of Cas9 nuclease toxicity and the presence of a recognition site for an endogenous A. woodii restriction–modification (R-M) system in the cas9 gene. As an alternative, this study aims to facilitate the exploitation of CRISPR/Cas endogenous systems as genome engineering tools. Accordingly, a Python script was developed to automate the prediction of protospacer adjacent motif (PAM) sequences and used to identify PAM candidates of the A. woodii Type I-B CRISPR/Cas system. The identified PAMs and the native leader sequence were characterized in vivo by interference assay and RT-qPCR, respectively. Expression of synthetic CRISPR arrays, consisting of the native leader sequence, direct repeats, and adequate spacer, along with an editing template for homologous recombination, successfully led to the creation of 300 bp and 354 bp in-frame deletions of pyrE and pheA, respectively. To further validate the method, a 3.2 kb deletion of hsdR1 was also generated, as well as the knock-in of the fluorescence-activating and absorption-shifting tag (FAST) reporter gene at the pheA locus. Homology arm length, cell density, and the amount of DNA used for transformation were found to significantly impact editing efficiencies. The devised workflow was subsequently applied to the Type I-B CRISPR/Cas system of Clostridium autoethanogenum, enabling the generation of a 561 bp in-frame deletion of pyrE with 100% editing efficiency. This is the first report of genome engineering of both A. woodii and C. autoethanogenum using their endogenous CRISPR/Cas systems.
A review on the role of CASC11 in cancers
Authors: Ghafouri-Fard Soudeh , Harsij Atefeh , Hussen Bashdar Mahmud , Taheri Mohammad , Sharifi Guive
Published: June 2023

The long non-coding RNA (lncRNA) cancer susceptibility 11 (CASC11) is a newly identified lncRNA located on chromosome 8q24.21. The expression of lncRNA CASC11 has been found to be elevated in different cancer types and the prognosis of the tumor is inversely correlated with the high CASC11 expression. Moreover, lncRNA CASC11 has an oncogenic function in cancers. The biological characteristics of the tumors, such as proliferation, migration, invasion, autophagy, and apoptosis can be controlled by this lncRNA. In addition to interacting with miRNAs, proteins, transcription factors, and other molecules, the lncRNA CASC11 modulates signaling pathways including Wnt/β-catenin and epithelial-mesenchymal transition. In this review, we have summarized studies on the role of lncRNA CASC11 in the carcinogenesis from cell lines, in vivo, and clinical perspectives.
The role of the ERK signaling pathway in promoting angiogenesis for treating ischemic diseases
Authors: Song Yue-Yue , Liang Dan , Liu De-Kun , Lin Lin , Zhang Lei , Yang Wen-Qing
Published: June 2023

The main treatment strategy for ischemic diseases caused by conditions such as poor blood vessel formation or abnormal blood vessels involves repairing vascular damage and encouraging angiogenesis. One of the mitogen-activated protein kinase (MAPK) signaling pathways, the extracellular signal-regulated kinase (ERK) pathway, is followed by a tertiary enzymatic cascade of MAPKs that promotes angiogenesis, cell growth, and proliferation through a phosphorylation response. The mechanism by which ERK alleviates the ischemic state is not fully understood. Significant evidence suggests that the ERK signaling pathway plays a critical role in the occurrence and development of ischemic diseases. This review briefly describes the mechanisms underlying ERK-mediated angiogenesis in the treatment of ischemic diseases. Studies have shown that many drugs treat ischemic diseases by regulating the ERK signaling pathway to promote angiogenesis. The prospect of regulating the ERK signaling pathway in ischemic disorders is promising, and the development of drugs that specifically act on the ERK pathway may be a key target for promoting angiogenesis in the treatment of ischemic diseases.
Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
Authors: Li Min , Sun Jin , Shi Guohai
Published: June 2023

Tumor is one of the biggest threats to human health. Though tumor therapy has been dramatically advanced by the progress of technology and research in recent decades, it is still far from expectations. Thus, it is of great significance to explore the mechanisms of tumor growth, metastasis, and resistance. Screen based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein (Cas) 9 gene editing technology are powerful tools for exploring the abovementioned facets. This review summarizes the recent screen performed in cancer cells and immune cells in the tumor microenvironment. The screens in cancer cells mainly focus on exploring the mechanisms underlying cancer cells’ growth, metastasis, and how cancer cells escape from the FDA approved drugs or immunotherapy. And the studies in tumor-associated immune cells are primarily aimed at identifying signaling pathways that can enhance the anti-tumor function of cytotoxic T lymphocytes (CTLs), CAR-T cells, and macrophages. Moreover, we discuss the limitations, merits of the CRISPR screen, and further its future application in tumor studies. Importantly, recent advances in high throughput tumor related CRISPR screen have deeply contributed to new concepts and mechanisms underlying tumor development, tumor drug resistance, and tumor immune therapy, all of which will eventually potentiate the clinical therapy for tumor patients.
Monoclonal antibodies against lipopolysaccharide protect against Pseudomonas aeruginosa challenge in mice
Authors: Kang Jason , Mateu-Borrás Margalida , Monroe Hunter L. , Sen-Kilic Emel , Miller Sarah Jo , Dublin Spencer R. , Huckaby Annalisa B. , Yang Evita , Pyles Gage M. , Nunley Mason A. , Chapman Josh A. , Amin Md Shahrier , Damron F. Heath , Barbier Mariette
Published: June 2023

Pseudomonas aeruginosa is a common cause of hospital-acquired infections, including central line-associated bloodstream infections and ventilator-associated pneumonia. Unfortunately, effective control of these infections can be difficult, in part due to the prevalence of multi-drug resistant strains of P. aeruginosa. There remains a need for novel therapeutic interventions against P. aeruginosa, and the use of monoclonal antibodies (mAb) is a promising alternative strategy to current standard of care treatments such as antibiotics. To develop mAbs against P. aeruginosa, we utilized ammonium metavanadate, which induces cell envelope stress responses and upregulates polysaccharide expression. Mice were immunized with P. aeruginosa grown with ammonium metavanadate and we developed two IgG2b mAbs, WVDC-0357 and WVDC-0496, directed against the O-antigen lipopolysaccharide of P. aeruginosa. Functional assays revealed that WVDC-0357 and WVDC-0496 directly reduced the viability of P. aeruginosa and mediated bacterial agglutination. In a lethal sepsis model of infection, prophylactic treatment of mice with WVDC-0357 and WVDC-0496 at doses as low as 15 mg/kg conferred 100% survival against challenge. In both sepsis and acute pneumonia models of infection, treatment with WVDC-0357 and WVDC-0496 significantly reduced bacterial burden and inflammatory cytokine production post-challenge. Furthermore, histopathological examination of the lungs revealed that WVDC-0357 and WVDC-0496 reduced inflammatory cell infiltration. Overall, our results indicate that mAbs directed against lipopolysaccharide are a promising therapy for the treatment and prevention of P. aeruginosa infections.