bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the impact of FDI and environmental regulation on the industrial structure upgrading in the Yellow River Basin
Authors: Yu Jinxiu
Published: July 2024

Introductions: Since the reform and opening up, the inflow of foreign direct investment (FDI) has provided a steady stream of capital, technology, talent and other resources for the development of the Yellow River basin, while caused problems such as environmental pollution, ecological fragility and industrial structure upgrading difficulties to some extent. Environmental regulation is a pivotal initiative to achieve mutual harmony between ecological environment and economic development, which could enhance the quality of the introduction of FDI and accelerate the green transformation of the development mode.

Methods: Based on urban panel data from 2006–2019, this study empirically examined the impact of FDI and environmental regulation on industrial structure upgrading in the Yellow River Basin. Moreover, taking environmental regulation as a threshold variable, a panel threshold model was established to further explore the role of environmental regulation in the impact of FDI on industrial structure upgrading in the Yellow River Basin.

Results: (1) The relationship between FDI and industrial structure upgrading in the Yellow River Basin is not a simple linear relationship, but an inverted “U”-shaped relationship that rises first and then falls, and the results of this inverted “U”-shaped relationship are still robust after replacing key indicators. (2) The environmental regulation policy has a driving effect on the upgrading of industrial structure in the Yellow River Basin. (3) Environmental regulation has a positive role in the influence of FDI on the industrial structure upgrading in the Yellow River basin, and the positive role increases gradually as the intensity of environmental regulation increases moderately, but if the intensity of environmental regulation is too high, it will have a negative impact on the upgrading of industrial structure in the Yellow River basin to some extent.

Discussion: In the future, policymakers should make reasonable and effective use of FDI and improve the quality of FDI; reasonably formulate environmental regulation policies; coordinate the intensity of FDI and environmental regulation; thus, bring into play the promotion effect of FDI and environmental regulation on industrial structure upgrading, and then realize the win-win of ecological protection and high-quality economic development in the Yellow River Basin.
Asylum spaces as spaces of nonrecognition: the ambiguities of welcoming and educating asylum-seeking children in Norway
Authors: Kalisha Wills
Published: July 2024

Asylum has historically been a refuge for the persecuted. However, it now faces a fundamental antagonism between asylum and law, which often overlooks the rights of children seeking asylum. This paper explores the historical concept of asylum law from its inception in antiquity and the Roman era and its entanglements with the education of children. It also examines asylum’s relevance to current conflicts over the educational rights of asylum-seeking children and how international conventions have neglected these rights. To contextualize the ambiguity of asylum and education, the paper presents a case study of Norway’s reception of asylum-seeking children from the 1930s to the present day and how the Norwegian welfare system, which was founded on child rights protection, has struggled with the entry of unaccompanied asylum-seeking children. The paper concludes with reflections on the potential impact of education for these children, considering their encounters as temporary educational moments. This exploration focuses on clarifying historical strands, turning points, and threads rather than taking a subjective historical perspective.
Comparable cognitive impairment was detected in MACS and CS and alleviated after remission of hypercortisolism in MACS
Authors: Liu Mengsi , Zhao Wenji , Zhang Wei , Tian Zhaoyang , Zhang Zhou , Lou Yuan , Zhang Ziwei , Yang Fan , Zhu Dalong , Li Ping
Published: July 2024

Context

Few studies have directly compared the cognitive characteristics of patients with mild autonomous cortisol secretion (MACS) and Cushing’s syndrome (CS). The effect of surgical or conservative treatment on cognitive function in patients with MACS is still unclear.

Objective

To compare the differences in cognitive function between patients with MACS and CS and evaluate the effect of surgery or conservative treatment on cognitive function.

Methods

We prospectively recruited 59 patients with nonfunctional adrenal adenoma (NFA), 36 patients with MACS, and 20 patients with adrenal CS who completed the global cognition and cognitive subdomains assessments. Seventeen MACS patients were re-evaluated for cognitive function after a 12-month follow-up period; of these, eleven underwent laparoscopic adrenalectomy and six received conservative treatment.

Results

Patients with MACS and CS performed worse in the global cognition and multiple cognitive domains than those with NFA (all P<0.05). No statistical difference was found in cognitive functions between patients with MACS and CS. Logistic regression analysis showed that patients with MACS (odds ratio [OR]=3.738, 95% confidence intervals [CI]: 1.329–10.515, P=0.012) and CS (OR=6.026, 95% CI: 1.411–25.730, P=0.015) were associated with an increased risk of immediate memory impairment. Visuospatial/constructional, immediate and delayed memory scores of MACS patients were significantly improved at 12 months compared with pre-operation in the surgical treatment group (all P<0.05), whereas there was no improvement in the conservative treatment group.

Conclusion

Patients with MACS have comparable cognitive impairment as patients with CS. Cognitive function was partially improved in patients with MACS after adrenalectomy. The current data support the inclusion of cognitive function assessment in the clinical management of patients with MACS.
Simulation and evaluation of ecosystem service value along the Yellow River in Henan Province, China
Authors: Zhao Dong , Guo Lanbo , Chen Guolong , Yan Lijie , Sun Tingting
Published: July 2024

The unprecedented growth in population and swift industrial advancements exert considerable strains on the ecosystem, particularly within medium-sized and large urban landscapes. The critical investigation into the intricate links between current and prospective land utilization, as well as the ecosystem service value (ESV), holds considerable empirical relevance for the calibration of land usage frameworks, thereby contributing to the sustainable evolution of extensive urban zones. Utilizing GlobeLand 30 data, the present research probes into the pattern of land transformation and the spatial-temporal dispersal of ESV in Henan’s Yellow River vicinity over a span from 2000 to 2020. For the enhancement of land usage alignment, a Markov-PLUS fusion model was devised to gauge three disparate ESV transition scenarios slated for 2030, namely, natural development scenario (NDS), cropland protection scenario (CPS), and ecological protection scenario (EPS). The principal determinants of land transformation within the 2000–2020 period were recognized as elevation, populace concentration, and atmospheric temperature. Amid the rapid accretion of construction land engulfing substantial cropland and grassland areas, there was an ESV diminution to the tune of 1.432 billion RMB between 2000 and 2020. The ESV’s high-value regions were discerned within relatively undisturbed ecosystem zones, with the lower-value sections identified in cropland and constructed areas, where human interventions exerted pronounced effects on the ecosystem. In accordance with the 2030 land usage simulations and analyses, in contrast to alternative scenarios, the EPS exhibited the least fluctuation in land type alterations in 2030, demonstrated the most pronounced escalation in cold spot concentration, and reached a peak agglomeration level. This underscores that the EPS not only offers a refinement in land utilization configuration but also mediates the equilibrium between economic and ecological considerations. The insights derived from this investigation afford innovative evaluative methods for spatial planning, ecological recompense, and sustainable land exploitation within large- and medium-scale urban domains.
Genetic parameters for pelvic organ prolapse in purebred and crossbred sows
Authors: Chen Ching-Yi , Knap Pieter W. , Bhatnagar Adria S. , Tsuruta Shogo , Lourenco Daniela , Misztal Ignacy , Holl Justin W.
Published: July 2024

This study aimed to investigate genetic parameters for sow pelvic organ prolapse in purebred and crossbred herds. Pelvic organ prolapse was recorded as normal or prolapsed on the individual sow level across 32 purebred and 8 crossbred farms. In total, 75,162 purebred Landrace sows from a single maternal line were recorded between 2018 and 2023, while 18,988 commercial two-way crossbred (Landrace x Large White) sows were available between 2020 and 2023. There were 5,122,005 animals included in the pedigree. The prolapse in purebreds and crossbreds was considered two different traits in the model. Pedigrees of the crossbred sows were determined based on genotypes through parentage assignment. The average incidence rates were 1.81% and 3.93% for purebreds and crossbreds, respectively. The bivariate model incorporated fixed effects of parity group and region with random effects of contemporary group (farm and mating year and month at the first parity), additive genetic, and residual. Genetic parameter estimates were obtained using BLUPF90+ with the AIREML option. The estimated additive variance was larger in crossbreds than in purebreds. Estimates of heritability in the observed scale were 0.09 (0.006) for purebreds and 0.11 (0.014) for crossbreds, with a genetic correlation of 0.83 using a linear model. Results suggested that including data from crossbreds with higher incidence rate is beneficial and selection to reduce the prolapse incidence in purebred sow herds would also benefit commercial crossbred sow herds.
Exercise-downregulated CD300E acted as a negative prognostic implication and tumor-promoted role in pan-cancer
Authors: Luo Zhiwen , Zhu Jinguo , Xu Rui , Wan Renwen , He Yanwei , Chen Yisheng , Wang Qing , Chen Shuo , Chen Shiyi
Published: July 2024

Background

Breast cancer ranks as one of the most prevalent malignancies among women globally, with increasing incidence rates. Physical activity, particularly exercise, has emerged as a potentially significant modifier of cancer prognosis, influencing tumor biology and patient outcomes.

Methods

Using a murine breast cancer model, we established a control and an exercise group, where the latter was subjected to 21 days of voluntary running. RNA Sequencing, bioinformatics analysis, pan-cancer analysis, and cell experiments were performed to validate the underlying mechanisms.

Results

We observed that exercise significantly reduced tumor size and weight, without notable changes in body weight, suggesting that physical activity can modulate tumor dynamics. mRNA sequencing post-exercise revealed substantial downregulation of CD300E in the exercise group, accompanied by alterations in critical pathways such as MicroRNAs in cancers and the Calcium signaling pathway. Expanding our analysis to a broader cancer spectrum, CD300E demonstrated significant expression variability across multiple cancer types, with pronounced upregulation in myeloma, ovarian, lung, and colorectal cancers. This upregulation was correlated with poorer prognostic outcomes, emphasizing CD300E’s potential role as a prognostic marker and therapeutic target. Moreover, CD300E expression was associated with cancer cell proliferation and apoptosis.

Conclusion

The study highlights the dual role of exercise in modulating gene expression relevant to tumor growth and the potential of CD300E as a target in cancer therapeutics. Further research is encouraged to explore the mechanisms by which exercise and CD300E influence cancer progression and to develop targeted strategies that could enhance patient outcomes in clinical settings.
Epigenetic regulation of human FOXP3+ Tregs: from homeostasis maintenance to pathogen defense
Authors: Yue Yi , Ren Yuqing , Lu Chunya , Li Ping , Zhang Guojun
Published: July 2024

Regulatory T cells (Tregs), characterized by the expression of Forkhead Box P3 (FOXP3), constitute a distinct subset of T cells crucial for immune regulation. Tregs can exert direct and indirect control over immune homeostasis by releasing inhibitory factors or differentiating into Th-like Treg (Th-Treg), thereby actively contributing to the prevention and treatment of autoimmune diseases. The epigenetic regulation of FOXP3, encompassing DNA methylation, histone modifications, and post-translational modifications, governs the development and optimal suppressive function of Tregs. In addition, Tregs can also possess the ability to maintain homeostasis in diverse microenvironments through non-suppressive mechanisms. In this review, we primarily focus on elucidating the epigenetic regulation of Tregs as well as their multifaceted roles within diverse physiological contexts while looking forward to potential strategies involving augmentation or suppression of Tregs activity for disease management, particularly in light of the ongoing global COVID-19 pandemic.
Multi-component synthesis and invitro biological assessment of novel pyrrole derivatives and pyrano[2,3-c]pyrazole derivatives using Co3O4 nanoparticles as recyclable nanocatalyst
Authors: Almeleebia Tahani M. , Jasim Naser Mokhtar , Saeed Shakir Mahmood , Abid Majeed M. , Altimari Usama S. , Shaghnab Murtadha Laftah , Rasen Fadhil A. , Alawadi Ahmed , Ahmad Irfan , Alsalamy Ali
Published: July 2024

In this study, Co3O4 nanoparticles were used as nanocatalyst for two different series of nitrogen-containing heterocyclic compounds, including pyrrole (Pyo) derivatives and pyrano [2, 3-c]pyrazole (Pya[2, 3-c]Pyz) derivatives. In the synthesis of derivatives, using 15 mol% and 10 mol% of the catalyst for Pyo derivatives and Pya[2, 3-c]Pyz derivatives, respectively, an efficiency between 83% and 96%, were observed. In addition, novel derivatives of Pyo and Pya[2,3-c]Pyz were synthesized and their structures were confirmed. In general, the advantages of using cobalt nanoparticles compared to previous reports include the synthesis of new derivatives, lower temperature used in the synthesis of derivatives, shorter synthesis time and high efficiency. The biological properties of the synthesized products, such as antibacterial, antifungal, and antioxidant properties, were tested and investigated. In antibacterial and antifungal tests, IZD, MIC, MBC, and MFC were measured and reported. In antioxidant activity, IC50 was calculated and reported. High reusability, green and environmentally friendly, synthesis of new derivatives and synthesis of products with higher efficiency and shorter time were the important benefits of using cobalt nanoparticles as a catalyst. In antioxidant tests, the IC50 for synthesized Pyo derivatives and Pya[2, 3-c] Pyz derivatives were between 12.2 and 13.71 μg/mL, and 16.18–17.75 μg/mL, respectively. In antimicrobial testes, the MIC for synthesized Pyo derivatives and Pya[2, 3-c]Pyz derivatives were between 2 and 4,096 μg/mL, and 2–2048 μg/mL, respectively. The results showed that the antioxidant property of Pyo derivatives were more than Pya[2, 3-c] Pyz derivatives, but the antimicrobial effect of Pya[2,3-c] Pyz derivatives were more than Pyo derivatives. The antioxidant results proved that the activity of Pyo derivatives and Pya[2, 3-c] Pyz derivatives does not depend on the substitutions of the derivatives and is close to each other. Therefore, based on this, a proposed mechanism for stability of DPPH by Pyo derivatives and Pya[2, 3-c] Pyz derivatives were suggested. Finally, based on the more stable resonance structures of Pyo derivatives, compared to Pya[2, 3-c] Pyz derivatives, its high antioxidant property was justified. Pya[2, 3-c] Pyz derivatives has two heterocyclic rings connected together pyrano and pyrazole, but Pyo derivatives has only one heterocyclic ring (pyrrole). So high antimicrobial property of Pya[2, 3-c] Pyz derivatives compared to Pyo derivatives can be attributed to having two bioactive heterocyclic rings.
Design and performance evaluation of a compact radiation absorber for 5G applications
Authors: Khan Imran , Alshehri Asma , Wang Pi-Chung , Hameed Ibrahim A.
Published: July 2024

Introduction: The emergence of electromagnetic wave pollution as a new form of pollution in human society is attributed to the advancements in communication technology and the electronic information business. In addition to harming priceless electronic equipment, these electromagnetic radiation and interference issues brought on by electrical and electronic devices have a major negative influence on human productivity and wellbeing. The secret to getting rid of electromagnetic radiation interference (EMI) and improving performance is electromagnetic shielding technology. Metamaterial absorber is a type of metamaterial that absorb EMI radiation. The benefits of metamaterial absorbers include their lightweight, simple construction, and excellent absorptivity.

Methods: This paper proposes a novel metamaterial absorber for EMI radiation absorption. It consists of dielectric layers, metamaterial shielding layer and transmission line. The reflection of radiation is reduced by miniaturization of metamaterials.

Results and Discussion: Simulation results show that the proposed design has better performance as compared to existing methods. The operating frequency range is from 23.1 to 28.3 GHz. The values of S21 with and without shielding are −5 dB and −0.05 dB, and the shielding effectiveness is 10.10 dB and a maximum of 12.63 dB.
Unusual adult-onset cardiac rhabdomyomas in tuberous sclerosis complex: a case report
Authors: Nati-Castillo H. A. , Quintero Juan Manuel , Molina Oswaldo Aguilar , Arias-Intriago Marlon , Melgar Muñoz Fernando P. , Izquierdo-Condoy Juan S.
Published: July 2024

Background

Tuberous sclerosis complex is a genetic neurocutaneous autosomal dominant syndrome, characterized by the development of multiple benign tumors (hamartomas) affecting various systems. Heart-benign tumors that result from the complex are called cardiac rhabdomyomas. Unlike hamartomas that occur in other organs, cardiac rhabdomyomas are most prevalent in infants and very young children with tuberous sclerosis complex. We present a case of a young adult with tuberous sclerosis who had an unusually late diagnosis of cardiac rhabdomyomas.

Case report

A 22-year-old male patient of Afro-descendant, diagnosed with tuberous sclerosis complex in childhood, presented with refractory epilepsy and was treated only with lacosamide. The patient came to medical consultation due to a recent history of episodic, persistent chest pain in the sternal region, associated with physical effort. Echocardiography revealed a non-dilated left ventricle, with several rounded masses of high echogenicity without pedicles at the apical level, the largest measuring 14 × 11 mm, consistent with cardiac rhabdomyomas.

Conclusion

Cardiac rhabdomyomas rarely develop in adulthood for individuals with tuberous sclerosis. These late-onset cases can exhibit various symptoms, from simple to complex presentations. Regular clinical checkups are essential for adults with tuberous sclerosis complex.
Exosome prospects in the diagnosis and treatment of non-alcoholic fatty liver disease
Authors: Tamimi Amirhossein , Javid Mona , Sedighi-Pirsaraei Nasrin , Mirdamadi Arian
Published: July 2024

The growing prevalence of NAFLD and its global health burden have provoked considerable research on possible diagnostic and therapeutic options for NAFLD. Although various pathophysiological mechanisms and genetic factors have been identified to be associated with NAFLD, its treatment remains challenging. In recent years, exosomes have attracted widespread attention for their role in metabolic dysfunctions and their efficacy as pathological biomarkers. Exosomes have also shown tremendous potential in treating a variety of disorders. With increasing evidence supporting the significant role of exosomes in NAFLD pathogenesis, their theragnostic potential has become a point of interest in NAFLD. Expectedly, exosome-based treatment strategies have shown promise in the prevention and amelioration of NAFLD in preclinical studies. However, there are still serious challenges in preparing, standardizing, and applying exosome-based therapies as a routine clinical option that should be overcome. Due to the great potential of this novel theragnostic agent in NAFLD, further investigations on their safety, clinical efficacy, and application standardization are highly recommended.
Toxin-antitoxin system gene mutations driving Mycobacterium tuberculosis transmission revealed by whole genome sequencing
Authors: Hou Yawei , Li Yifan , Tao Ningning , Kong Xianglong , Li Yameng , Liu Yao , Li Huaichen , Wang Zhenguo
Published: July 2024

Background

The toxin-antitoxin (TA) system plays a vital role in the virulence and pathogenicity of Mycobacterium tuberculosis (M. tuberculosis). However, the regulatory mechanisms and the impact of gene mutations on M. tuberculosis transmission remain poorly understood.

Objective

To investigate the influence of gene mutations in the toxin-antitoxin system on M. tuberculosis transmission dynamics.

Method

We performed whole-genome sequencing on the analyzed strains of M. tuberculosis. The genes associated with the toxin-antitoxin system were obtained from the National Center for Biotechnology Information (NCBI) Gene database. Mutations correlating with enhanced transmission within the genes were identified by using random forest, gradient boosting decision tree, and generalized linear mixed models.

Results

A total of 13,518 M. tuberculosis isolates were analyzed, with 42.29% (n = 5,717) found to be part of genomic clusters. Lineage 4 accounted for the majority of isolates (n = 6488, 48%), followed by lineage 2 (n = 5133, 37.97%). 23 single nucleotide polymorphisms (SNPs) showed a positive correlation with clustering, including vapB1 G34A, vapB24 A76C, vapB2 T171C, mazF2 C85T, mazE2 G104A, vapB31 T112C, relB T226A, vapB11 C54T, mazE5 T344C, vapB14 A29G, parE1 (C103T, C88T), and parD1 C134T. Six SNPs, including vapB6 A29C, vapB31 T112C, parD1 C134T, vapB37 G205C, Rv2653c A80C, and vapB22 C167T, were associated with transmission clades across different countries. Notably, our findings highlighted the positive association of vapB6 A29C, vapB31 T112C, parD1 C134T, vapB37 G205C, vapB19 C188T, and Rv2653c A80C with transmission clades across diverse regions. Furthermore, our analysis identified 32 SNPs that exhibited significant associations with clade size.

Conclusion

Our study presents potential associations between mutations in genes related to the toxin-antitoxin system and the transmission dynamics of M. tuberculosis. However, it is important to acknowledge the presence of confounding factors and limitations in our study. Further research is required to establish causation and assess the functional significance of these mutations. These findings provide a foundation for future investigations and the formulation of strategies aimed at controlling TB transmission.
Progressive remodeling of structural networks following surgery for operculo-insular epilepsy
Authors: Obaid Sami , Guberman Guido I. , St-Onge Etienne , Campbell Emma , Edde Manon , Lamsam Layton , Bouthillier Alain , Weil Alexander G. , Daducci Alessandro , Rheault François , Nguyen Dang K. , Descoteaux Maxime
Published: July 2024

Introduction

Operculo-insular epilepsy (OIE) is a rare condition amenable to surgery in well-selected cases. Despite the high rate of neurological complications associated with OIE surgery, most postoperative deficits recover fully and rapidly. We provide insights into this peculiar pattern of functional recovery by investigating the longitudinal reorganization of structural networks after surgery for OIE in 10 patients.

Methods

Structural T1 and diffusion-weighted MRIs were performed before surgery (t0) and at 6 months (t1) and 12 months (t2) postoperatively. These images were processed with an original, comprehensive structural connectivity pipeline. Using our method, we performed comparisons between the t0 and t1 timepoints and between the t1 and t2 timepoints to characterize the progressive structural remodeling.

Results

We found a widespread pattern of postoperative changes primarily in the surgical hemisphere, most of which consisted of reductions in connectivity strength (CS) and regional graph theoretic measures (rGTM) that reflect local connectivity. We also observed increases in CS and rGTMs predominantly in regions located near the resection cavity and in the contralateral healthy hemisphere. Finally, most structural changes arose in the first six months following surgery (i.e., between t0 and t1).

Discussion

To our knowledge, this study provides the first description of postoperative structural connectivity changes following surgery for OIE. The ipsilateral reductions in connectivity unveiled by our analysis may result from the reversal of seizure-related structural alterations following postoperative seizure control. Moreover, the strengthening of connections in peri-resection areas and in the contralateral hemisphere may be compatible with compensatory structural plasticity, a process that could contribute to the recovery of functions seen following operculo-insular resections for focal epilepsy.
Identification of genetic susceptibility for Chinese migraine with depression using machine learning
Authors: An Xingkai , Zhao Shanshan , Fang Jie , Li Qingfang , Yue Cen , Jing Chuya , Zhang Yidan , Zhang Jiawei , Zhou Jie , Chen Caihong , Qu Hongli , Ma Qilin , Lin Qing
Published: July 2024

Background

Migraine is a common primary headache that has a significant impact on patients’ quality of life. The co-occurrence of migraine and depression is frequent, resulting in more complex symptoms and a poorer prognosis. The evidence suggests that depression and migraine comorbidity share a polygenic genetic background.

Objective

The aim of this study is to identify related genetic variants that contribute to genetic susceptibility to migraine with and without depression in a Chinese cohort.

Methods

In this case-control study, 263 individuals with migraines and 223 race-matched controls were included. Eight genetic polymorphism loci selected from the GWAS were genotyped using Sequenom’s MALDI-TOF iPLEX platform.

Results

In univariate analysis, ANKDD1B rs904743 showed significant differences in genotype and allele distribution between migraineurs and controls. Furthermore, a machine learning approach was used to perform multivariate analysis. The results of the Random Forest algorithm indicated that ANKDD1B rs904743 was a significant risk factor for migraine susceptibility in China. Additionally, subgroup analysis by the Boruta algorithm showed a significant association between this SNP and migraine comorbid depression. Migraineurs with depression have been observed to have worse scores on the Beck Anxiety Inventory (BAI) and the Migraine Disability Assessment Scale (MIDAS).

Conclusion

The study indicates that there is an association between ANKDD1B rs904743 and susceptibility to migraine with and without depression in Chinese patients.
The impacts of sex and the 5xFAD model of Alzheimer’s disease on the sleep and spatial learning responses to feeding time
Authors: Campbell Katrina J. , Jiang Peng , Olker Christopher , Lin Xuanyi , Kim Sarah Y. , Lee Christopher J. , Song Eun Joo , Turek Fred W. , Vitaterna Martha Hotz
Published: July 2024

Introduction

The relationships between the feeding rhythm, sleep and cognition in Alzheimer’s disease (AD) are incompletely understood, but meal time could provide an easy-to-implement method of curtailing disease-associated disruptions in sleep and cognition. Furthermore, known sex differences in AD incidence could relate to sex differences in circadian rhythm/sleep/cognition interactions.

Methods

The 5xFAD transgenic mouse model of AD and non-transgenic wild-type controls were studied. Both female and male mice were used. Food access was restricted each day to either the 12-h light phase (light-fed groups) or the 12-h dark phase (dark-fed groups). Sleep (electroencephalographic/electromyographic) recording and cognitive behavior measures were collected.

Results

The 5xFAD genotype reduces NREM and REM as well as the number of sleep spindles. In wild-type mice, light-fed groups had disrupted vigilance state amounts, characteristics, and rhythms relative to dark-fed groups. These feeding time differences were reduced in 5xFAD mice. Sex modulates these effects. 5xFAD mice display poorer spatial memory that, in female mice, is curtailed by dark phase feeding. Similarly, female 5xFAD mice have decreased anxiety-associated behavior. These emotional and cognitive measures are correlated with REM amount.

Discussion

Our study demonstrates that the timing of feeding can alter many aspects of wake, NREM and REM. Unexpectedly, 5xFAD mice are less sensitive to these feeding time effects. 5xFAD mice demonstrate deficits in cognition which are correlated with REM, suggesting that this circadian-timed aspect of sleep may link feeding time and cognition. Sex plays an important role in regulating the impact of feeding time on sleep and cognition in both wild-type and 5xFAD mice, with females showing a greater cognitive response to feeding time than males.
A bibliometric analysis and visualization of normal pressure hydrocephalus
Authors: Chang Tengwu , Huang Xiaoyuan , Zhang Xu , Li JinYong , Bai Wenju , Wang Jichao
Published: July 2024

Background

Normal pressure hydrocephalus (NPH) has drawn an increasing amount of attention over the last 20 years. At present, there is a shortage of intuitive analysis on the trends in development, key contributors, and research hotspots topics in the NPH field. This study aims to analyze the evolution of NPH research, evaluate publications both qualitatively and quantitatively, and summarize the current research hotspots.

Methods

A bibliometric analysis was conducted on data retrieved from the Web of Science Core Collection (WoSCC) database between 2003 and 2023. Quantitative assessments were conducted using bibliometric analysis tools such as VOSviewer and CiteSpace software.

Results

A total of 2,248 articles published between 2003 and 2023 were retrieved. During this period, the number of publications steadily increased. The United States was the largest contributor. The University of Gothenburg led among institutions conducting relevant research. Eide P. K. was the most prolific author. The Journal of Neurosurgery is the leading journal on NPH. According to the analysis of the co-occurrence of keywords and co-cited references, the primary research directions identified were pathophysiology, precise diagnosis, and individualized treatment. Recent research hotspots have mainly focused on epidemiology, the glymphatic system, and CSF biomarkers.

Conclusion

The comprehensive bibliometric analysis of NPH offers insights into the main research directions, highlights key countries, contributors, and journals, and identifies significant research hotspots. This information serves as a valuable reference for scholars to further study NPH.
No causal relationship between serum vitamin D levels and alcoholic liver disease: a two-sample bidirectional Mendelian randomization study
Authors: Wu Huan , Wu Long , Zhang Quan , Li Can , Li Hai-yang , Zhang Bao-fang
Published: July 2024

Background

Numerous observational studies have presented an association between Vitamin D (VD) and Alcoholic Liver Disease (ALD). However, sufficient evidence from Randomized Controlled Trials (RCTs) substantiating this correlation is scarce, thus leaving the causality of this relationship ambiguous. To overcome the shortcomings of traditional observational studies, we performed a two-sample bidirectional Mendelian randomization (MR) analysis to ascertain the causal relationship between VD and ALD.

Methods

We utilized summary statistics datasets from Genome-Wide Association Studies (GWAS) for VD and ALD. We selected genetic instruments that measure circulating VD levels (n = 64,979), and retrieved ALD statistics from GWASs, inclusive of 1,416 cases and 217,376 healthy controls, while excluding chronic liver diseases such as nonalcoholic fatty liver disease, toxic liver disease, and viral hepatitis. Subsequent, MR analyses were performed to obtain effect estimates using inverse variance weighted (IVW) random effect models. Cochran’s Q statistic and MR-Egger regression intercept analyses were used to assess pleiotropy. Sensitivity analyses using the MR Egger, weighted median, simple mode, and weighted mode methods were also performed. Leave-one-out analysis was used to identify SNPs with potential effect. Reverse MR analysis was also performed.

Results

In IVW, our MR analysis incorporated 21 independent SNPs, circulating VD levels had no causal effect on ALD [OR = 0.624 (0.336–1.160), p = 0.136] and ALD had no causal effect on circulating VD [OR = 0.997 (0.986–1.008), p = 0.555]. No heterogeneity or pleiotropy was observed (p > 0.05). Other MR methods also agreed with IVW results.

Conclusion

This study provides the causal relationship between genetically predicted circulating Vitamin D levels and ALD and provides new insights into the genetics of ALD.
Differences in cooking taste and physicochemical properties between compound nutritional rice and common rice
Authors: Zhang Dongmeng , Li Jian , Yao Dongping , Wu Jun , Luo Qiuhong , Shen Hong , Hu Meixia , Meng Fudie , Zhang Ying , Liu Xionglun , Shan Yang , Liu Dongbo , Bai Bin
Published: July 2024

In this study, it was compared the physicochemical properties and cooking taste quality between four different types of compound nutritional rice (rice flour with the addition of other coarse grains, legumes, potatoes, and other powders, extruded as artificial rice grains) and common rice. We found that the protein and apparent amylose contents of compound nutritional rice were higher than that of common rice, up to 9.775% and 19.45% respectively. The γ-aminobutyric acid (GABA) and resistant starch contents were much lower than in common rice, and the dietary fiber content did not differ from that in common rice. The results of Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis revealed that the starch properties and structure of the compound nutritional rice changed due to high temperature and high pressure processing. In particular, the crystalline structures of starch became V-shaped. In addition, the results of artificial tasting and tasting meter showed that the taste of compound nutritional rice was generally inferior to that of common rice. In summary, compound nutritional rice had problems such as nutritional imbalance and poor taste. There was still a lot of room for improving the taste quality of compound nutritional rice. Therefore, the future development of compound nutritional rice should focus on both nutritional balance and taste improvement. The results of this paper also provided a certain theoretical basis for this.
Case report: An intriguing case of Philadelphia chromosome–positive acute lymphoblastic leukemia recurrence
Authors: Peccatori Nicolò , Chiocca Elena , Conter Valentino , Tondo Annalisa , Marzorati Matilde , Casini Tommaso , Veltroni Marinella , Biondi Andrea , Fazio Grazia
Published: July 2024

The incorporation of tyrosine kinase inhibitors (TKIs) in the treatment of Philadelphia chromosome–positive acute lymphoblastic leukemia (Ph+ ALL) led to significant improvement. However, in the pediatric setting, the outcomes of Ph+ ALL are still inferior compared to those of other ALL subtypes even in the TKI era due to higher relapse rate. Herein, we report a very peculiar case of late extramedullary Ph+ ALL relapse in a child, characterized by lymphomatous presentation in the tonsils and lymphoid lineage switch. The diagnostic dilemma between the occurrence of a second malignant neoplasm and the recurrence of the primary disease is further discussed, highlighting the importance of molecular backtracking analysis. This case report emphasizes the high plasticity and polyclonal nature of ALL and expands the heterogeneity of possible clinical presentation of Ph+ ALL at relapse.
Safety assessment of neurokinin-1 receptor antagonist: real-world adverse event analysis from the FAERS database
Authors: Yang Chuanli , Xu Pengyang , Wu Teng , Fan Yunhe , Li Qingqing , Zhang Jijun , Shen Xiaobing , Dong Xiushan
Published: July 2024

Background

Aprepitant, fosaprepitant, and netupitant are three common neurokinin-1 receptor antagonists (NK-1RAs) used to prevent chemotherapy-induced nausea and vomiting, following highly or moderately emetogenic chemotherapy. Understanding their different adverse event (AE) profiles may help clinicians make appropriate treatment decisions.

Methods

All data collected from the FDA Adverse Event Reporting System (FAERS) database from the first quarter of 2004 to the fourth quarter of 2023 underwent disproportionality analysis to detect, evaluate, and compare AE signals of the three NK-1RAs.

Results

A total of 3,904, 1,123, and 243 AE reports related to aprepitant, fosaprepitant, and netupitant, respectively, were extracted from the FAERS database. Of these, more than 50% of respondents were female, and most of them were aged 45–65 years. General disorders and administration-site conditions, and gastrointestinal disorders were the most frequent signals in the system organ class of the three NK-1RA drugs. In addition, aprepitant was strongly associated with joint deposit (ROR = 26.27) and fosaprepitant was closely related to seizure-like phenomena (ROR = 26.90); two preferred terms (PTs) were not mentioned in the manual. Statistically, netupitant was likely to induce death (N = 63, ROR = 8.78, 95% CI: 6.75–11.42). Additionally, neutropenic colitis, colitis, and stomatitis were unique to netupitant. Furthermore, the AE profiles of the three NK-1RA drugs were different by gender.

Conclusion

The AE profiles for aprepitant, fosaprepitant, and netupitant were different. In addition to paying attention to common AEs, clinicians need to pay attention to new emerging AEs, such as joint deposit, seizure-like phenomena, neutropenic colitis, colitis, and stomatitis, regarding the three NK-1RA drugs. Furthermore, the AE compositions of the three NK-1RA drugs were different in different genders, and clinicians should take these factors into account when selecting NK-1RAs for CINV treatment.
Status and hotspot analysis of Qingfei Paidu Decoction for the prevention and treatment of COVID-19 based on bibliometric analysis
Authors: Zhang Huifang , Liu Yang , Shang Xiyu , Cao Yuqing , Li Jiajia , Chen Guangkun , Ji Xinyu , Zhang Lei , Fan Yipin , Ma Yan
Published: July 2024

Background

Qingfei Paidu Decoction (QFPDD) has played an important role in the prevention and treatment of COVID-19 infection in China. The present study aims to perform an econometric analysis and visualization of the literature on the treatment of COVID-19 with QFPDD in the Chinese databases and English databases.

Methods

Six databases including such as Chinese databases CNKI, VIP, CBM, WANFANG as well as English databases PubMed, Web of Science were searched for publications related to the prevention and treatment of COVID-19 with QFPDD. The institutions, authors, keywords of each publication were cisualized using the software of CiteSpace.

Results

A total of 187 literature on the prevention and treatment of novel coronavirus infection with QFPDD were included, of which 145 (77.5%) were in Chinese and 42 (22.5%) were in English. Those publications were written by 926 authors from 383 institutions. There were 78 theoretical studies (41.7%), 63 clinical studies (33.7%), and 46 basic studies (24.6%). The cooperative institutions with the core of “Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences” and “Shanghai University of Chinese Medicine Cross Academy of Science” have been formed, and two core teams with “Wang Yanping” and “Zhang Weidong” have been formed. The keyword analysis showed that the research mainly focuses on pathologic pathogenesis, clinical efficacy, adverse reactions, integrated Chinese and western medicine therapy, network pharmacology research.

Conclusion

QFPDD has attracted worldwide attention, mechanism research and clinical research may become a future development trend. Therefore, in-depth basic research and clinical studies with large samples and multi-center cooperation should be carried out to provide high-level evidence-based evidence for the prevention and treatment of COVID-19 with QFPDD.
Therapeutic potential of Chinese medicinal herbs stimulating osteogenic differentiation of bone marrow-derived mesenchymal stem cells in osteoporosis
Authors: Wang Hui , Shan Kai , Li Yan , Wu Sinuo , Zhou Chunman , Tao Shan , Wang Meijuan , Kang Xiaochun , Zhou Liang , Lyu Zhongxi , Li Ningcen
Published: July 2024

Osteoporosis (OP) is a common and complex chronic metabolic disease with an increasing incidence rate, which has markedly increased the human health burden worldwide. The predominant cause of OP is an imbalance between osteoblasts (OB) and osteoclasts (OC). Studies on the correlation between bone marrow-derived mesenchymal stem cells (BMSCs) and OP have indicated that BMSCs-induced OB differentiation is an important pathway for bone tissue renewal. Chinese medicinal herbs have been used for centuries to treat various types of OPs because they are safer and more effective. The in vivo and in vitro experiments have confirmed that these herbs or their primary phytochemicals may exert therapeutic effects by stimulating BMSCs differentiation, which restores OB and OP balance, inhibits adipocyte differentiation, exerts anti-inflammatory and antioxidant effects, regulates the immune system, etc. This review summarizes the research on how Chinese medicinal herbs or their primary phytochemicals treat OP by stimulating BMSC differentiation and provides a scientifically reliable basis and perspective for their future clinical application.
The galectin-3 inhibitor selvigaltin reduces liver inflammation and fibrosis in a high fat diet rabbit model of metabolic-associated steatohepatitis
Authors: Comeglio Paolo , Guarnieri Giulia , Filippi Sandra , Cellai Ilaria , Acciai Gabriele , Holyer Ian , Zetterberg Fredrik , Leffler Hakon , Kahl-Knutson Barbro , Sarchielli Erica , Morelli Annamaria , Maggi Mario , Slack Robert J. , Vignozzi Linda
Published: July 2024

Introduction

Galectin-3 is a pro-fibrotic β-galactoside binding lectin highly expressed in fibrotic liver and implicated in hepatic fibrosis. Selvigaltin (previously known as GB1211) is a novel orally active galectin-3 small molecule inhibitor that has high affinity for galectin-3 (human KD = 25 nM; rabbit KD = 12 nM) and high oral bioavailability in rabbits and man. In this study the efficacy of selvigaltin was investigated in a high fat diet (HFD) rabbit model of metabolic-associated steatohepatitis (MASH).

Methods

Male New Zealand White rabbits were individually caged under standard conditions in a temperature and humidity-controlled room on a 12 h light/darkness cycle. After 1 week of regular diet (RD), rabbits were randomly assigned for 8 or 12 weeks to different groups: RD/vehicle, RD/selvigaltin, HFD (8 weeks), HFD/vehicle and HFD/selvigaltin (0.3, 1.0, 5.0 or 30 mg/kg selvigaltin with vehicle/selvigaltin p.o. dosed therapeutically q.d. 5 days per week from week 9 or 12). Liver inflammation, steatosis, ballooning, and fibrosis was measured via blood metabolic markers, histomorphological evaluation [Oil Red O, Giemsa, Masson’s trichome, picrosirius red (PSR) and second harmonic generation (SHG)], and mRNA and protein expression.

Results

Steatosis, inflammation, ballooning, and fibrosis were all increased from RD to HFD/vehicle groups. Selvigaltin demonstrated target engagement by significantly decreasing galectin-3 levels in the liver as measured via immunohistochemistry and mRNA analysis. Selvigaltin dose-dependently reduced biomarkers of liver function (AST, ALT, bilirubin), inflammation (cells foci), and fibrosis (PSR, SHG), as well as decreasing the mRNA and protein expression of several key inflammation and fibrosis biomarkers (e.g., IL6, TGFβ3, SNAI2, collagen). Doses of 1.0 or 5.0 mg/kg demonstrated consistent efficacy across most biological endpoints supporting the current clinical doses of selvigaltin being investigated in liver disease.

Discussion

Selvigaltin significantly reduced hepatic inflammation and fibrosis in an HFD rabbit model of MASH following therapeutic dosing for 4 weeks in a dose-dependent manner. These data support the human selvigaltin dose of 100 mg b.i.d. that has been shown to reduce key liver biomarkers during a clinical study in liver cirrhosis.
Date palm transcriptome analysis provides new insights on changes in response to high salt stress of colonized roots with the endophytic fungus Piriformospora indica
Authors: Ahmad Manzoor , Abdul Aziz Mughair , Sabeem Miloofer , Kutty M. Sangeeta , Sivasankaran Sathesh K. , Brini Faical , Xiao Ting Ting , Blilou Ikram , Masmoudi Khaled
Published: July 2024

Salinity is a significant threat that causes considerable yield losses in date palm. The root endophytic fungus Piriformospora indica has proven effective in providing salt stress tolerance to host plants. However, the underlying molecular mechanism facilitating the date palm’s response to P. indica inoculation, and its involvement in the salt stress tolerance, remains unknown. In this study, the colonization of P. indica on date palm seedlings exposed to saline conditions was observed through confocal microscopy, and its impact on gene expressions was evaluated using the transcriptomic analysis. Our findings show that P. indica colonization reinforced the cortical cells, prevented them from plasmolysis and cell death under salinity. The RNAseq analysis produced clean reads ranging from 62,040,451 to 3,652,095 across the treatment groups, successfully assembling into 30,600 annotated genes. Out of them, the number of differentially expressed genes (DEGs) varied across the treatments: i.e., 2523, 2031, and 1936 DEGs were upregulated, while 2323, 959, and 3546 were downregulated in Salt, Fungi, and Fungi+Salt groups, respectively. Furthermore, principal component analysis based on transcriptome profiles revealed discrete clustering of samples from different treatment groups. KEGG and GO pathways enrichment analysis highlighted variation in the number and types of enriched pathways among the treatments. Our study indicated variations in gene expression related to plant hormone biosynthesis and signal transduction (auxin, abscisic acid, gibberellin, and ethylene), ABC transporters, sodium/hydrogen exchanger, cation HKT transporter, transcription factors such as WRKY and MYBs, and the plant immune system (lipoxygenase and jasmonate) of the date palm seedlings. By characterizing the transcriptome of date palm roots under salt stress and with colonization of P. indica, the present findings provide valuable perspectives on the molecular mechanisms responsible for inducing salinity stress tolerance in plants.