bluuebunny/crossref_metadata_2025 huggingface dataset:
E-Learning for Continuing Professional Development of University Librarians: A Systematic Review
Authors: Shahzad Khurram , Khan Shakeel Ahmad , Javed Yasir , Iqbal Abid
Published: January 2023

Evidence from well-established theories of various disciplines manifests that, in the current technology-led world, continued professional development (CPD) of information professionals plays a paramount role in the uplift of institutions. CPD of university library professionals via e-learning programs leads to the implementation of user-centric-services through the initiation of emerging technological tools and the latest methods of service-delivery. The focus of this study is to shed light on the factors influencing e-learning for CPD of working librarians, challenges being encountered for e-learning adoption, and to propose the best practices for designing an efficient e-learning portfolio. For meeting the focused study-objectives, the authors applied PRISMA guidelines and procedures. An extensive search was conducted utilizing the world’s 16 leading e-databases and digital tools containing the most relevant core studies. Consequently, 30 impact factor research papers published in renowned databases were included through the identification, screening, eligibility, and inclusion process. Findings revealed that different factors—including organizational survival, continuous changes, adoption of emerging technologies, and professional growth—encouraged e-learning for CPD of information professionals. The study results showed that four main challenges—technical difficulties, lack of funds, reliance upon conventional models, and overwhelming work-load—were encountered for e-learning adoption. The authors proposed a framework for the development of an effective and efficient e-learning portfolio for building professional expertise among university librarians to support the organizational vision and mission statement. The recommended framework is based upon emergent categories and sub-categories extracted via thematic analysis of the existing empirical studies. This study has theoretical insights for the researchers through valuable addition in the body of literature and practical considerations for policy implementers to construct sustainable policies for devising e-learning programs to develop professional expertise in the university library workforce for attainment of value-added outcomes.
Optimal Feeder Reconfiguration and Placement of Voltage Regulators in Electrical Distribution Networks Using a Linear Mathematical Model
Authors: Gallego Pareja Luis A. , López-Lezama Jesús M. , Gómez Carmona Oscar
Published: January 2023

Power distribution systems face continuous challenges from increased demand and lengthening of feeders, resulting in power loss augmentation and unacceptable voltage drops. Thus, to reduce technical losses and improve the voltage profile, common techniques such as reactive compensation, network reconfiguration, and placing of voltage regulators are employed. Distribution network reconfiguration (DNR) consists of modifying the system topology with the aim of minimizing power losses, enhancing voltage profile, and improving network reliability. Optimal placement of voltage regulators (OPVRs) improves the voltage profile and helps to reduce power losses. DNR and OPVRs are challenging optimization problems involving both integer and continuous decision variables. In this paper, a mixed-integer linear programming (MILP) model is presented to simultaneously solve the problems of DNR and OPVRs in radial distribution networks. The combined optimal DNR and OPVRs aim at both the minimization of power losses and the improvement of the voltage profile. This approach has not been reported in the specialized literature. The proposed MILP model may be solved through commercially available software, obtaining global optimal solutions with lower computational effort than metaheuristic techniques applied for the same purpose. Several tests were conducted on three benchmark distribution test systems to demonstrate the efficacy and applicability of the proposed approach.
Hydraulically Disconnected Rivers in the Highlands and Southern Riverine Plain of S.E Australia
Authors: Macumber Phillip G.
Published: January 2023

The rivers of south-eastern Australia flow within a complex meander tract (Coonambidgal Formation) formed by phases of Quaternary stream activity. Pumping tests, hydrochemistry and groundwater monitoring of the Campaspe, Loddon and Murray River Valleys show that for significant parts of their courses, the rivers and their associated strip aquifers form a single integrated hydraulic unit perched above and disconnected from the regional water table by an underlying aquitard developed at the top of a varyingly thick and temporally dynamic vadose zone. Loss to the regional aquifer is not restricted to the riverbed but covers the entire width of the Coonambidgal Formation aquifer, which is one or two orders of magnitude greater. River-bed flux is not a measure of net river loss. Through diffusion and dispersion from the overlying saturated zone, aquitard enhancement or development is augmented by chemical processes active towards the top of the vadose zone. Unlike river-bed clogging, chemical clogging of aquifers is progressive and permanent. Post-European instability in the studied groundwater systems has seen catchment wide groundwater rises of up to 0.25 m/y. or 25 metre over the last century. Under the pre-existing norm of deeper water tables, disconnected streams would have been more numerous with the present aquitards being a legacy of that regime.
Enhancing the Biodiesel Production Potential of Synechococcus elongatus and Anabaena Cyanobacterial Strain Isolated from Saline Water Using Different Media Composition and Organic Carbon Sources
Authors: Parthiban Jeevitha , Jambulingam Ranjitha
Published: January 2023

In the present study, Synechococcus elongatus and Anabaena, two cyanobacterial species were cultured using different media conditions such as ASN III, modified ASN III, BG-11, and BBM for the enrichment of biomass and lipid productivity. The experimental result clearly shows that BG 11 was the efficient and cost-effective medium for both the isolated cyanobacterial species such as Synechococcus elongatus and Anabaena. The influence of organic carbon sources on biomass and lipid productivity of the selected cyanobacterial species were studied when cultivated in a BG-11 medium using different organic carbon sources such as sucrose, glucose, sodium acetate and glycerol under mixotrophic conditions. Based on the experimental results, the isolated cyanobacterial strain Synechococcus elongatus and Anabaena showed an enriching effect on lipid production under mixotrophic conditions, but whereas Synechococcus elongatus showed a significant effect three times greater lipid productivity compared with Anabaena cyanobacterial strain, by the addition of glycerol as a supplement to the culture media.
Entrepreneurial Potential and Agribusiness Desirability among Youths in South Kivu, Democratic Republic of the Congo
Authors: Simbeko Guy , Nguezet Paul-Martin Dontsop , Sekabira Haruna , Yami Mastewal , Masirika Serge Amato , Bheenick Krishan , Bugandwa Deogratias , Nyamuhirwa Dieu-Merci Akonkwa , Mignouna Jacob , Bamba Zoumana , Manyong Victor
Published: January 2023

In the Democratic Republic of Congo (DRC), entrepreneurship in the agriculture sector remains for youth a key pillar for income creation. However, few are attracted by agribusiness despite stakeholders’ efforts toward engaging youth in agriculture. Therefore, this study examines the relationship between entrepreneurial potential characteristics and youth desirability to start an enterprise in agriculture among 514 young people in Eastern DRC. This study revealed that youth in South Kivu have different entrepreneurship potential features and agribusiness desirability levels according to their gender and living area. Hence, the youth’s agribusiness desirability is motivated by an awareness of emerging agripreneurial activities, land ownership, parent involvement in farming activities as a role model, perceived agribusiness as an employment source, management-organizing and opportunistic competencies, market analysis, negotiating, and planning skills. Therefore, efforts to attract youth into agribusiness should focus on the use of media, the creation of awareness of available agribusiness initiatives in their area, and the setup of land policy. This is in addition to putting in place capacity-building programs on entrepreneurial and business skills through incubators, and the formalization of youth agribusiness groups that foster capitalizing experiences between new and accelerated agripreneurial enterprises, with the support of parents and financial institutions, focusing on gender sensitivity, in both rural and urban areas.
The Removal of Tetracycline from Aqueous Solutions Using Peanut Shell Biochars Prepared at Different Pyrolysis Temperatures
Authors: Shi Zhichao , Ma Aowen , Chen Yuanhang , Zhang Menghan , Zhang Yin , Zhou Na , Fan Shisuo , Wang Yi
Published: January 2023

The pyrolysis temperature strongly affects the properties of the peanut shell biochar, and influences its adsorption behavior and mechanisms for contaminant removal in aqueous solutions. In this study, peanut shells were pyrolyzed at 400 °C and 700 °C to prepare two biochars (PSBC400 and PSBC700), which were then characterized using scanning electron microscopy/X-ray energy spectrum analysis, Brunauer–Emmett–Teller, elemental analysis, X-ray fluorescence, X-ray diffraction, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. The adsorption behavior of typical tetracycline (TC) onto the biochars was investigated, and the potential adsorption mechanisms explored. The results show that compared with PSBC400, PSBC700 has a larger specific surface area and pore volume and contains higher levels of carbon and ash, but shows lower O, N, and H content. The hydrophilicity and polarity of PSBC700 is lower, but its aromaticity is higher. Furthermore, the mineral content of PSBC400 is higher than for PSBC700. The functional groups differ between PSBC400 and PSBC700, especially those containing C and O. The Elovich and two-compartment adsorption kinetic models are a good fit to the TC adsorption processes on both biochars, but the Langmuir adsorption isotherm model provides better results. The theoretical maximum adsorption capacities of TC onto PSBC700 and PSBC400 are 33.4346 mg·g−1 and 26.4185 mg·g−1, respectively. The main adsorption mechanisms of TC onto PSBC400 are hydrogen bonding and complexation, and are closely related to the functional groups and minerals found in PSBC400. In contrast, the main adsorption mechanisms of TC onto PSBC700 are pore filling and the π–π interaction, and are mainly determined by the surface area and graphited carbon structure of PSBC700. In summary, effective biochar can be manufactured from peanut shell biomass and can be used to remove TC from aqueous solutions.
The Effect of Grafting on Morphological, Physiological and Molecular Changes Induced by Drought Stress in Cucumber
Authors: Coşkun Ömer Faruk
Published: January 2023

Drought is one of the most important abiotic stress factors affecting crop yields and qualities worldwide. One drought-sensitive plant is the cucumber, which has a high transpiration rate. Grafting is recognized as a promising approach to increasing tolerance to abiotic stresses in cucumber. In this study, it is aimed to determine the rootstock that will prevent negative changes in some growth, physiological and genetic parameters of cucumber under drought stress and to determine the mechanism of the system. In drought stress conditions, leaf number values were found to be 6 on average in non-grafted plants and between 6–13.16 in grafted plants. Average leaf fresh weight values (7.56–9.84 g) obtained from grafted plants were higher than non-grafted plants (5.7 g). Leaf chlorophyll content (SPAD) values were found to be between 24.43 in non-grafted plants and 37.83–55.36 in grafted plants under stress conditions. Malondialdehyde (MDA) concentration values also decreased from 5.66 to 3.23–4.36 in grafted plants. It was determined that the genomic template stability (GTS) rate was 64.1% in the non-grafted treatment group. DNA polymorphisms detected by ISSR (inter simple sequence repeat) can be used as a biomarker system for the detection of genotoxic effects of abiotic stresses, such as drought. These findings suggest that grafting with drought-resistant rootstocks may improve drought tolerance in drought-sensitive cucumber genotypes.
Facilitating Circular Economy Strategies Using Digital Construction Tools: Framework Development
Authors: Jemal Kebir Mohammed , Kabzhassarova Marzhan , Shaimkhanov Ramazan , Dikhanbayeva Dinara , Turkyilmaz Ali , Durdyev Serdar , Karaca Ferhat
Published: January 2023

The construction sector has undergone several transformations to address adverse environmental, economic, and social impacts. The concept of the circular economy (CE) has transcended into this domain to solve the needs of construction amid resource constraints. Furthermore, advanced digital tools are being implemented across industries owing to the boost given by the fourth industrial revolution. This paper aims to develop a framework that investigates the effect of digital tools on CE implementation in the construction sector. The study is based on a three-step approach, where first, an initial framework design based on a systematic literature review was conducted. This is followed by framework optimization using semistructured interviews with experts and validation through a case study. This study resulted in the development of a new framework, which aims to investigate how advanced digital tools can be used in the construction sector to enhance CE implementation. The contribution of the present study is two-fold: (1) the integration (addressing existing research gap) of CE and digitalization concepts in the construction sector; (2) an investigation into the critical barriers, offering insights for construction practitioners.
Experimental Study on Tensile Characteristics of Layered Carbonaceous Slate Subject to Water–Rock Interaction and Weathering
Authors: Li Erqiang , Wei Yanqing , Chen Zhanyang , Archbold Paul , Mullarney Brian
Published: January 2023

The transverse isotropy of rock masses formed by sedimentation is a common stratum environment in engineering, and the physical–mechanical properties can degrade due to water–rock interaction (WRI) and natural weathering, which potentially lead to the instability or collapse of tunneling, slopes and mining. Taking the carbonaceous slate of the Muzhailing tunnel as the research object, two types of specimens, which include oven-drying (instant drying in oven after fabrication) and natural air-drying (static weathering for 60 days after fabrication) were prepared, respectively, after which Brazilian tests were carried out and the tensile properties were analyzed under the two conditions. The experimental results showed that the two kinds of carbonaceous slate all show brittle failure, but the mechanical response such as failure displacement and peak load is obviously different. The tensile strength of the specimens is significantly all affected by the bedding, while the cleavage failure patterns of the two kinds are affected to different degrees. The softening coefficient of the natural air-drying specimen is 0.11–0.13, which implies that WRI and natural weathering play a vital role in the course of rock failure but have little influence on the transverse isotropy tensile property of bedding. Moreover, the mechanisms of specimen failure subject to WRI and 60 days’ weathering were explained by the SEM technique, which analyzed the micro-components and observes the process of specimen deterioration due to physicochemical reaction, the gradual development of cracks and erosion by weathering.
SWCNTs/PEDOT: PSS Coated Cotton for Wearable Clothes and Supercapacitor Applications
Authors: Badawi Nujud Mohammad , Batoo Khalid Mujasam , Ramesh S. , Ramesh K. , Imran Ahamad
Published: January 2023

Herein, we report single-wall carbon nanotubes (SWCNT)/poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT: PSS) loading on the transparency and conductivity of pure cotton and systematically studied using a four-probe stack made of copper (Cu) which showed a surface resistance of 0.08 Ω/cm. Moreover, the treated cotton cloth retained its maximum resistance even after three months. Surface morphology was investigated by scanning electron microscopy (SEM) and elemental structure analysis was performed by energy-dispersive X-ray (EDX), while the structural analysis was performed using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) techniques, confirming that there is a good dispersion of SWCNTs/PEDOT: PSS in the cotton sample. The composite cotton/hydrogel polymer/composite cotton achieved a specific capacitance of 212.16 F/g at 50 mV/s. Thermal properties were also investigated using thermogravimetric analysis (TGA) and differential scale calorimetry (DSC). The low surface resistance and thermal stability show that cotton fabric can be a promising candidate for smart wearable textiles and modern circuitry applications.
Potential Use of Coriander Waste as Fuel for the Generation of Electric Power
Authors: Rojas-Flores Segundo , De La Cruz-Noriega Magaly , Cabanillas-Chirinos Luis , Nazario-Naveda Renny , Gallozzo-Cardenas Moisés , Diaz Félix , Murga-Torres Emzon
Published: January 2023

The increase in the population and its need to produce food has caused the level of contamination by organic waste to increase exponentially in recent years. Innovative methods have been proposed for the use of this waste and thus to mitigate its impact. One of these is to use it as fuel in microbial fuel cells to generate electricity. This research aims to generate bioelectricity using coriander waste in microbial fuel cells. The maximum voltage and current observed were 0.882 ± 0.154 V and 2.287 ± 0.072 mA on the seventh and tenth day, respectively, these values were obtained working at an optimum operating pH of 3.9 ± 0.16 and with an electrical conductivity of 160.42 ± 4.54 mS/cm. The internal resistance observed in the cells was 75.581 ± 5.892 Ω, with a power density of 304.325 ± 16.51 mW/cm2 at 5.06 A/cm2 current density. While the intensity of the final FTIR (Fourier transform infrared spectroscopy) spectrum peaks decreased compared to the initial one, likewise, with a percentage of identity, it was possible to attribute 98.97, 99.39, and 100% to the species Alcaligenes faecalis, Alcaligenes faecali, and Pseudomonas aeruginosa. Finally, the cells were connected in series, managing to turn on an LED light (red) with the 2.61 V generated. This research provides an innovative and environmentally friendly way that companies and farmers can use to reuse their waste.
Optimising the Distribution of Multi-Cycle Emergency Supplies after a Disaster
Authors: Wang Fuyu , Ge Xuefei , Li Yan , Zheng Jingjing , Zheng Weichen
Published: January 2023

In order to achieve rapid and fair distribution of emergency supplies after a large-scale sudden disaster, this paper constructs a comprehensive time perception satisfaction function and a comprehensive material loss pain function to portray the perceived satisfaction of disaster victims based on objective constraints such as limited transport, multimodal transport and supply being less than demand, and at the same time considers the subjective perception of time and material quantity of disaster victims under limited rational conditions, and constructs a multi-objective optimisation model for the dispatch of multi-cycle emergency supplies by combining comprehensive rescue cost information. For the characteristics of the proposed model, based on the NSGA-II algorithm, generalized reverse learning strategy, coding repair strategy, improved adaptive crossover, variation strategy, and elite retention strategy are introduced. Based on this, we use the real data of the 2008 Wenchuan earthquake combined with simulated data to design corresponding cases for validation and comparison with the basic NSGA-II algorithm, SPEA-II and MOPSO algorithms. The results show that the proposed model and algorithm can effectively solve the large-scale post-disaster emergency resource allocation problem, and the improved NSGA- II algorithm has better performance.
A Comprehensive Review on Land Use/Land Cover (LULC) Change Modeling for Urban Development: Current Status and Future Prospects
Authors: Gaur Srishti , Singh Rajendra
Published: January 2023

Land use land cover (LULC) modeling is considered as the best tool to comprehend and unravel the dynamics of future urban expansion. The present paper provides a comprehensive review of existing LULC modeling techniques and novel approaches used by the research community. Moreover, the review also compares each technique’s applications, utility, drawbacks, and broader differences. The rationale behind such a comparison is to highlight the strengths/weakness of individual techniques. The review further highlights the utility of the hybridization of different techniques (e.g., machine learning model combined with statistical models) to LULC modeling to complement their strengths. Although significant progress has been made in LULC modeling, the review highlights the need to incorporate the policy framework into LULC modeling for better urban planning and management. The present review will help researchers and policymakers to achieve better land management practices and ultimately assist in achieving Sustainable Development Goal-15 (SDG-15) (i.e., life on land).
Corrosion Mechanisms of 304L NAG in Boiling 9M HNO3 Containing Cr (VI) Ions
Authors: Khan Shagufta , Saeed Adil , Nazir Mian Hammad , Abdullah Muhammad Usman , Khan Zulfiqar Ahmad
Published: January 2023

In this research, the mechanisms of end-grain corrosion of 304L NAG tubes in boiling 9M HNO3-containing Cr (VI) ions are reported to sustainably manage the corrosion of nuclear fuel reprocessing plant components. Specific heat treatments were applied to as-received specimens to produce phosphorus and/or sulphur intergranular segregation. End-grain corrosion on heat-treated specimens and the effect of a Cr (VI) concentration on a 304L NAG tube (as-received) were investigated. It has been reported that an increase in Cr (VI) ions leads to the acceleration of end-grain corrosion due to high electrochemical potential. After systematic heat treatments on the 304L NAG specimens, it is concluded that the primary causes of heat-induced end-grain corrosion are phosphorus or sulphur segregation to the grain boundaries. The key findings of this research are highly significant in terms of understanding the corrosion mechanisms and controlling the end-grain corrosion of NAG steel in boiling HNO3 environments. This research will help to sustainably reduce power plant maintenance costs and will have a significant impact on the delivery of long-term, clean, secure, and tenable energy.
Analysis of Factors Influencing the Corporate Performance of Listed Companies in China’s Agriculture and Forestry Sector Based on a Panel Threshold Model
Authors: Sun Yong , Liu Hui , Liu Jiwei , Sun Mingyu , Li Qun
Published: January 2023

The global food crisis caused by COVID-19 and the Russia–Ukraine conflict have made many countries around the world realize the significance of agroforestry to a country’s food security. However, China’s agroforestry R&D innovation is currently lagging behind in development, and some agricultural seeds are heavily dependent on foreign countries, which seriously affects China’s national food security. It is especially important to explore the reasons why China’s agroforestry R&D and innovation is lagging behind. As listed agroforestry companies face the market demand directly, there is an urgent need to study the R&D innovations of listed agroforestry companies at present. This paper analyzes the impacts of R&D innovation, corporate management and supply chain management on the corporate performance of listed agroforestry companies using the entropy weighting method, GMM estimation and panel threshold model, mainly by selecting annual panel data from CSMAR for the period 2010 to 2021. The following conclusions were drawn: (1) There is a nonlinear relationship between R&D innovation and firm performance, and a “U”-shaped relationship. This indicates that there is an entrance threshold for R&D innovation in the agroforestry industry, below which corporate performance does not improve. (2) There is a nonlinear relationship between corporate management and corporate performance, and a U-shaped relationship. (3) There is a nonlinear relationship between supply chain management and firm performance, with an inverted-U-shaped relationship. This paper explains the reasons for the slow development of R&D innovation in China’s agriculture and forestry industry and fills the gap in the theoretical study of the nonlinear relationship between R&D innovation and corporate performance of listed companies in China’s agriculture and forestry industry. Finally, this paper provides a theoretical basis for the decision making of government departments related to agriculture and forestry, and offers some suggestions for listed companies in agriculture and forestry to improve their corporate performance.
Application of HSMAAOA Algorithm in Flood Control Optimal Operation of Reservoir Groups
Authors: He Ji , Guo Xiaoqi , Chen Haitao , Chai Fuxin , Liu Shengming , Zhang Hongping , Zang Wenbin , Wang Songlin
Published: January 2023

The joint flood control operation of reservoir groups is a complex engineering problem with a large number of constraints and interdependent decision variables. Its solution has the characteristics of strong constraint, multi-stage, nonlinearity, and high dimension. In order to solve this problem, this paper proposes a hybrid slime mold and arithmetic optimization algorithm (HSMAAOA) combining stochastic reverse learning. Since ancient times, harnessing the Yellow River has been a major event for the Chinese nation to rejuvenate the country and secure the country. Today, flood risk is still the greatest threat to the Yellow River basin. This paper chooses five reservoirs in the middle and lower reaches of the Yellow River as the research object, takes the water level of each reservoir in each period as the decision variable, and takes the peak clipping of Huayuankou control point as the objective to build an optimization model. Then, HSMAAOA is used to solve the problem, and the results are compared with those of the slime mold algorithm (SMA) and particle swarm optimization (PSO). The peak clipping rates of the three algorithms are 52.9% (HSMAAOA), 48.69% (SMA), and 47.55% (PSO), respectively. The results show that the HSMAAOA algorithm is better than other algorithms. This paper provides a new idea to solve the problem of the optimal operation of reservoir flood controls.
Aye: A Trusted Forensic Method for Firmware Tampering Attacks
Authors: Zhang Yipeng , Li Ye , Li Zhoujun
Published: January 2023

The Programmable Logic Controller (PLC) is located at the junction of the virtual network and physical reality in the Industrial Control System (ICS), which is vulnerable to attacks due to its weak security. Specifically, firmware tampering attacks take the firmware under the PLC operating system as the primary attack target. The firmware provides the bridge between PLC’s hardware and software, which means tampering against the firmware can be more destructive and harmful than other attacks. However, existing defense and forensics methods against firmware tampering attacks are asymmetrical, which directly leads to the proliferation of such attacks and the difficulty of forensic tracing. How to accurately, quickly, and efficiently conduct forensics for such attacks is an urgent problem. In this paper, we designed and implemented a reliable detection method based on Joint Test Action Group (JTAG) and memory comparison—Aye, which can detect mainstream firmware tampering attacks reliably. To determine the effectiveness and reliability of Aye, we selected a widely used PLC to observe Aye’s performance in defense and forensics by simulating the two latest PLC firmware tampering attack methods. The experimental results show that Aye can effectively defend against firmware tampering attacks, helping improve the efficiency and accuracy of such attack detection and forensics.
Ordered Leonardo Quadruple Numbers
Authors: Nurkan Semra Kaya , Güven İlkay Arslan
Published: January 2023

In this paper, we introduce a new quadruple number sequence by means of Leonardo numbers, which we call ordered Leonardo quadruple numbers. We determine the properties of ordered Leonardo quadruple numbers including relations with Leonardo, Fibonacci, and Lucas numbers. Symmetric and antisymmetric properties of Fibonacci numbers are used in the proofs. We attain some well-known identities, the Binet formula, and a generating function for these numbers. Finally, we provide illustrations of the identities.
Hawksbill Sea Turtle (Eretmochelys imbricata) Blood and Eggs Organochlorine Pesticides Concentrations and Embryonic Development in a Nesting Area (Yucatan Peninsula, Mexico)
Authors: Salvarani Patricia I. , Vieira Luis R. , Rendón-von Osten Jaime , Morgado Fernando
Published: January 2023

Environmental contaminants with chemical origins, such as organochlorine pesticides (OCPs) have major impacts on the health of marine animals, including sea turtles, due to the bioaccumulation of those substances by transference throughout the food chain. The effects of environmental pollution on the health of marine turtles are very important for management strategies and conservation. During recent decades, the south Gulf of Mexico and the Yucatan Peninsula have suffered from increasingly frequent disturbances from continental landmasses, river systems, urban wastewater runoff, port areas, tourism, industrial activities, pesticides from agricultural use, and other pollutants, such as metals, persistent organic pollutants (POPs) and hydrocarbons (from the oil industry activities), which contaminate water and sediments and worsen the environmental quality of the marine ecosystem in this region. In this study, we assessed the concentrations of OCPs in the blood and eggs of 60 hawksbill turtles (Eretmochelys imbricata) nesting at the Punta Xen turtle camp, and their effects on the nesting population’s reproductive performance: specifically, maternal transfer and embryonic development were analyzed. Hematologic characteristics, including packed cell volume, white blood cell count, red blood cell count, and haemoglobin levels, and plasma chemistry values, including creatinine, blood urea nitrogen, uric acid, triglyceride, total cholesterol and glucose, were also measured. The general health of the turtles in this study, as well as their levels of urea, serum creatinine, glucose, uric, acid, cholesterol, and triglyceride, fell within normal ranges and was similar to other normal values, which could indicate the turtles’ good energy levels and body conditions for nest-building activity, with all of the turtles able to successfully come ashore to nest. All the same, the obtained results also indicate that OCPs affect the nesting and reproductive performance of the hawksbill turtles, as well as their fertility and the development of the population of eggs and reproductive performance, specifically in terms of maternal transference and embryonic development. There were significant differences in the concentrations of OCPs (ΣHCHs and ΣDienes) between maternal blood and eggs, indicating that these chemicals are transferred from nesting females to eggs and, ultimately, to hatchlings. OCPs may, therefore, have an effect on the health and reproductive performance of hawksbill turtles, both in terms of their fertility and egg development. Conservation strategies need to be species-specific, due to differences in feeding, and address the reasons for any decline, focusing on regional assessments. Thus, accurate and comparable monitoring data are necessary, which requires the standardization of monitoring protocols.
Development of High-Throughput Sample Preparation Procedures for the Quantitative Determination of Aflatoxins in Biological Matrices of Chickens and Cattle Using UHPLC-MS/MS
Authors: De Baere Siegrid , Ochieng Phillis E. , Kemboi David C. , Scippo Marie-Louise , Okoth Sheila , Lindahl Johanna F. , Gathumbi James K. , Antonissen Gunther , Croubels Siska
Published: January 2023

Aflatoxins (AFs) frequently contaminate food and animal feeds, especially in (sub) tropical countries. If animals consume contaminated feeds, AFs (mainly aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1), G2 (AFG2) and their major metabolites aflatoxin M1 (AFM1) and M2 (AFM2)) can be transferred to edible tissues and products, such as eggs, liver and muscle tissue and milk, which ultimately can reach the human food chain. Currently, the European Union has established a maximum level for AFM1 in milk (0.05 µg kg−1). Dietary adsorbents, such as bentonite clay, have been used to reduce AFs exposure in animal husbandry and carry over to edible tissues and products. To investigate the efficacy of adding bentonite clay to animal diets in reducing the concentration of AFB1, AFB2, AFG1, AFG2, and the metabolites AFM1 and AFM2 in animal-derived foods (chicken muscle and liver, eggs, and cattle milk), chicken and cattle plasma and cattle ruminal fluid, a sensitive and selective ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method has been developed. High-throughput sample preparation procedures were optimized, allowing the analysis of 96 samples per analytical batch and consisted of a liquid extraction using 1% formic acid in acetonitrile, followed by a further clean-up using QuEChERS (muscle tissue), QuEChERS in combination with Oasis® Ostro (liver tissue), Oasis® Ostro (egg, plasma), and Oasis® PRiME HLB (milk, ruminal fluid). The different procedures were validated in accordance with European guidelines. As a proof-of-concept, the final methods were used to successfully determine AFs concentrations in chicken and cattle samples collected during feeding trials for efficacy and safety evaluation of mycotoxin detoxifiers to protect against AFs as well as their carry-over to animal products.
The Effect of Ascorbic Acid and Nicotinamide on Panton–Valentine Leukocidin Cytotoxicity: An Ex Vivo Study
Authors: AlSaleh Abdullah , Shahid Mohammed , Farid Eman , Bindayna Khalid
Published: January 2023

Background: Panton–Valentine Leukocidin sustains a strong cytotoxic activity, targeting immune cells and, consequently, perforating the plasma membrane and inducing cell death. The present study is aimed to examine the individual effect of ascorbic acid and nicotinamide on PVL cytotoxicity ex vivo, as well as their effect on granulocytes viability when treated with PVL. Materials and Methods: The PVL cytotoxicity assay was performed in triplicates using the commercial Cytotoxicity Detection Kit PLUS (LDH). LDH release was measured to determine cell damage and cell viability was measured via flow cytometry. Results and discussion: A clear reduction in PVL cytotoxicity was demonstrated (p < 0.001). Treatment with ascorbic acid at 5 mg/mL has shown a 3-fold reduction in PVL cytotoxicity; likewise, nicotinamide illustrated a 4-fold reduction in PVL cytotoxicity. Moreover, granulocytes’ viability after PVL treatment was maintained when incubated with 5 mg/mL of ascorbic acid and nicotinamide. Conclusions: our findings illustrated that ascorbic acid and nicotinamide exhibit an inhibitory effect on PVL cytotoxicity and promote cell viability, as the cytotoxic effect of the toxin is postulated to be neutralized by antioxidant incubation. Further investigations are needed to assess whether these antioxidants may be viable options in PVL cytotoxicity attenuation in PVL-associated diseases.
Effects of Storage Duration and Structures on Sesame Seed Germination, Mold Growth, and Mycotoxin Accumulation
Authors: Alemayehu Samuel , Abera Fetien Abay , Ayimut Kiros Meles , Darnell Ross , Mahroof Rizana , Harvey Jagger , Subramanyam Bhadriraju
Published: January 2023

Sesame is an important oil crop for the Ethiopian economy. However, the lack of adequate storage facilities results in significant losses of sesame seeds. This study was designed to compare the effects of storage conditions and the subsequent impact on sesame seed germination, mold growth, and mycotoxin accumulation over the storage period. The efficacy of two hermetic bags (1. Purdue Improved Crop Storage (PICS) bags and 2. Super GrainPro (SGP) bags) was directly compared to sesame storage in polypropylene (PP bags) and Jute bags. Storage conditions (oxygen, carbon dioxide, temperature, moisture content and relative humidity) of samples were analyzed in the laboratory in three replicates. Results showed that the oxygen concentrations dropped to 6.9% (±0.02) in PICS bags and 8.7% (±0.06) in SGP bags at the end of 6 months of storage. In non-hermetic bags Jute and PP), oxygen levels were close to atmospheric levels at 2-, 4-, and 6-month storage periods. In non-hermetic bags throughout storage, the amount of seed infection by mold constantly increased, and seed germination decreased. Sesame seeds stored in hermetic bags had 89.7% (±0.58) to 88.3% (±2.89) germination rates versus 61.67% (±2.08) for non-hermetic storage bags over the 6-month seed storage period. All mycotoxin levels increased over the same storage period, whereas comparative levels were much lower in hermetic bags after six months. Sesame seeds stored in both hermetic bags had the lowest level of tested mycotoxins, and levels among the SGP and PICS bags were not significantly different from one another. This study provides strong evidence indicating that hermetic storage structures such as PICS and SGP significantly affect temperature, humidity, moisture content, CO2 and oxygen levels resulting in the lowering of fungal growth and mycotoxin accumulation and effectively preserving stored sesame without relying on synthetic pesticides in Ethiopia.
Knowledge, Attitude and Practices towards the Prevention of Schistosomiasis Mansoni in an Endemic Area of Alagoas, Northeast Brazil
Authors: Santos Adriano José dos , Lima Shirley Verônica Melo Almeida , Sousa Alvaro Francisco Lopes de , Vasconcelos dos Santos Aytana , Santos Israel Gomes de Amorim , Bezerra Santos Márcio , Feitosa Vera Lucia Corrêa , Santos Allan Dantas dos , Primão Juliana Cristina Magnani , Andrade Denise de , Silva José Rodrigo Santos
Published: January 2023

We analyzed the knowledge, attitudes and practices (KAP) of schistosomiasis mansoni prevention in an endemic area of Brazil. This cross-sectional study was conducted between March and May 2021, with 412 participants living in the municipality of Feira Grande, Alagoas, Brazil. Data collection occurred through visits to the Health Center Urbano II and Massapê, through an interview with a structured questionnaire to identify the levels of KAP regarding schistosomiasis prevention. Of all respondents, 70.87% lived in rural areas, 22.66% reported a history of past schistosomiasis and 52.71% never participated in schistosomiasis control program actions. Factors associated with better KAP scores were being part of an older age group, not using rainwater and having no history of past schistosomiasis. Specifically, among the domains, attitude was the highest score and knowledge was the lowest. Participation in a health intervention program, knowing someone who had schistosomiasis and having been informed through a public health program seemed to have an important impact on the population’s KAP. Our results contributed to broadening perceptions about schistosomiasis prevention, highlighting the positive impacts that health programs and interventions have on disease control.
How to Strengthen Constraints on Non-Newtonian Gravity from Measuring the Lateral Casimir Force
Authors: Klimchitskaya Galina L. , Mostepanenko Vladimir M.
Published: January 2023

It has been known that in the nanometer interaction range the available experimental data do not exclude the Yukawa-type corrections to Newton’s gravitational law, which exceed the Newtonian gravitational force by many orders of magnitude. The strongest constraints on the parameters of Yukawa-type interaction in this interaction range follow from the experiments on neutron scattering and from measurements of the lateral and normal Casimir forces between corrugated surfaces. In this work, we demonstrate that by optimizing the experimental configuration at the expense of the higher corrugation amplitudes and smaller periods of corrugations it is possible to considerably strengthen the currently available constraints within the wide interaction range from 4.5 to 37 nm. We show that the maximum strengthening by more than a factor of 40 is reachable for the interaction range of 19 nm.
Combating Lassa Fever in West African Sub-Region: Progress, Challenges, and Future Perspectives
Authors: Aloke Chinyere , Obasi Nwogo Ajuka , Aja Patrick Maduabuchi , Emelike Chinedum Uche , Egwu Chinedu Ogbonnia , Jeje Olamide , Edeogu Chuks Oswald , Onisuru Olalekan Olugbenga , Orji Obasi Uche , Achilonu Ikechukwu
Published: January 2023

Lassa fever (LF) is a rodent-borne disease that threatens human health in the sub-region of West Africa where the zoonotic host of Lassa virus (LASV) is predominant. Currently, treatment options for LF are limited and since no preventive vaccine is approved for its infectivity, there is a high mortality rate in endemic areas. This narrative review explores the transmission, pathogenicity of LASV, advances, and challenges of different treatment options. Our findings indicate that genetic diversity among the different strains of LASV and their ability to circumvent the immune system poses a critical challenge to the development of LASV vaccines/therapeutics. Thus, understanding the biochemistry, physiology and genetic polymorphism of LASV, mechanism of evading host immunity are essential for development of effective LASV vaccines/therapeutics to combat this lethal viral disease. The LASV nucleoprotein (NP) is a novel target for therapeutics as it functions significantly in several aspects of the viral life cycle. Consequently, LASV NP inhibitors could be employed as effective therapeutics as they will potentially inhibit LASV replication. Effective preventive control measures, vaccine development, target validation, and repurposing of existing drugs, such as ribavirin, using activity or in silico-based and computational bioinformatics, would aid in the development of novel drugs for LF management.
Effect of EGCG Extracted from Green Tea against Largemouth Bass Virus Infection
Authors: Cheng Yuan , Liu Mingzhu , Yu Qing , Huang Shuaishuai , Han Shuyu , Shi Jingu , Wei Hongling , Zou Jianwei , Li Pengfei
Published: January 2023

(1) Background: Largemouth bass virus (LMBV) is a major viral pathogen in largemouth bass (Micropterus salmoides) aquaculture that often causes high mortality and heavy economic losses, thus developing treatments to combat this pathogen is of great commercial importance. Green tea is a well-known medicinal plant that contains active ingredients with antiviral, antibacterial, and other biological activities. The goals of this study were to explore the effect and mechanism of green tea source compounds on LMBV and provide data to serve as the basis for the screening of targeted drugs in the future. In this study, we evaluated the effects of the main component of green tea, epigallocatechin-3-gallate (EGCG), against LMBV infection. (2) Methods: The safe working concentration of EGCG was identified by cell viability detection and light microscopy. The antiviral activity and mechanism of action of EGCG against LMBV infection were evaluated with light microscopy, an aptamer 6-carboxy-fluorescein-based fluorescent molecular probe, and reverse transcription quantitative PCR. (3) Results: The safe working concentration of EGCG was ≤10 μg/mL. EGCG showed significant anti-LMBV infection activity in a concentration-dependent manner, and it also destroyed the structure of virus particles. EGCG impacted the binding of virus particles to cell receptors and virus invasion into the host cells. Inhibitory effects of EGCG on LMBV particles, LMBV binding to the host-cell membrane, and LMBV invasion were 84.89%, 98.99%, and 95.23%, respectively. Meanwhile, the effects of EGCG subsequently were verified in vivo. The fatality rate of the LMBV + EGCG group was significantly lower than that of the LMBV group. (4) Conclusions: Our results suggest that EGCG has effective antiviral properties against LMBV and may be a candidate for the effective treatment and control of LMBV infections in largemouth bass aquaculture.
Recombinant GPEHT Fusion Protein Derived from HTLV-1 Proteins with Alum Adjuvant Induces a High Immune Response in Mice
Authors: Jahantigh Hamid Reza , Stufano Angela , Koohpeyma Farhad , Nikbin Vajihe Sadat , Shahosseini Zahra , Lovreglio Piero
Published: January 2023

The human T-cell leukemia virus type 1 (HTLV-1) is a positive single-stranded RNA virus that belongs to the delta retrovirus family. As a result, a vaccine candidate that can be recognized by B cells and T cells is a good candidate for generating a durable immune response. Further, the GPEHT protein is a multi-epitope protein designed based on the Gag, Pol, Env, Hbz, and Tax proteins of HTLV-1. In developing a suitable and effective vaccine against HTLV-1, the selection of a designed protein (GPEHT) with the formulation of an alum adjuvant was conducted. In this study, we assessed the potential of a multi-epitope vaccine candidate for stimulating the immune response against HTLV-1. In assessing the type of stimulated immune reaction, total IgG, IgG1, and IgG2a isotypes, as well as the cytokines associated with Th1 (IFN-γ), Th2 (IL-4), and Th17 (IL-17), were analyzed. The outcomes showed that the particular antisera (total IgG) were more elevated in mice that received the GPEHT protein with the alum adjuvant than those in the PBS+Alum control. A subcutaneous vaccination with our chimera protein promoted high levels of IgG1 and IgG2a isotypes. Additionally, IFN-γ, IL-4, and IL-17 levels were significantly increased after spleen cell stimulation in mice that received the GPEHT protein. The immunogenic analyses revealed that the GPEHT vaccine candidate could generate humoral and cell-mediated immune reactions. Ultimately, this study suggests that GPEHT proteins developed with an alum adjuvant can soon be considered as a prospective vaccine to more accurately evaluate their protective efficacy against HTLV-1.