bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the Characteristic of the Electrical Contact Resistance of Strap Contacts Used in High Voltage Bushings
Authors: Cheng Jianwei , Zhao Linjie , Zhou Xiaoyu , Ren Ting , Jin Shoufeng , Xie Tao , Liu Peng , Peng Zongren , Wang Qingyu
Published: June 2023

As an important electrical connection component in electrical equipment, the strap contact is directly related to the long-term operation stability of equipment. The electrical contact resistance (ECR) of the electrical connection structure is an important indicator for evaluating the reliability of the electrical contact system. In this research, the theoretical calculation of ECR of the strap contacts used by the G-W theoretical model and the fractal theoretical model is improved and compared. After comparison with the experimental measurement values, the rationality and accuracy of the two theoretical models are discussed. The results show that when the load is small (1–3 N), the maximum error of G-W model is 41%, while the fractal model method has a maximum error of 9%. When the load is large (4–10 N), the results of the two are almost the same, and the errors are both within 14%. In summary, the error range of the fractal model is smaller and the change trend is closer to the experimental value, which is suitable for a relatively better ECR analytical calculation theory. The research results can provide a theoretical basis for research on the electrical contact performance of the electrical contact structure of electrical equipment.
Permanent Magnet Flux Linkage Analysis and Maximum Torque per Ampere (MTPA) Control of High Saturation IPMSM
Authors: Li Chengxu , Zhang Wenjuan , Gao Jian , Huang Shoudao
Published: June 2023

The maximum torque per ampere (MTPA) control is significant for improving the efficiency of the interior permanent magnet synchronous motor (IPMSM). However, for the high saturation IPMSM, the change of the permanent magnet (PM) flux linkage is more complicated, which can cause the MTPA control to deviate from the optimal solution. Therefore, an improved MTPA control method for the high saturation IPMSM is proposed in this paper. Compared with other methods, the proposed method improves the conventional models of flux linkage and torque by analyzing the nonlinear variation of the PM flux linkage with the dq-axis currents. Subsequently, an expression suitable for the MTPA control of high saturated IPMSM is derived based on the improved models. The proposed parameter fitting models are then fitted using data from 11 operating points and incorporated into the MTPA optimization algorithm to obtain the MTPA curve. Finally, the effectiveness of the proposed method in enhancing the control accuracy of the MTPA angle is verified through simulations and experiments.
Optimization of Thermodynamic Parameters of the Biological Hydrogen Methanation in a Trickle-Bed Reactor for the Conditioning of Biogas to Biomethane
Authors: Holl Elena , Oskina Anastasia , Baier Urs , Lemmer Andreas
Published: June 2023

The increased demand for resources and energy that is developing with rising global consumption represents a key challenge for our generation. Biogas production can contribute to sustainable energy production and closing nutrient cycles using organic residues or as part of a utilization cascade in the case of energy crops. Compared to hydrogen (H2), biogas with a high methane (CH4) content can be fed into the gas grid without restrictions. For this purpose, the CH4 content of the biogas must be increased from 52 to 60% after anaerobic digestion to more than 96%. In this study, biological hydrogen methanation (BHM) in trickling-bed reactors (TBR) is used to upgrade biogas. Design of experiments (DoE) is used to determine the optimal process parameters. The performance of the reactors is stable under all given conditions, reaching a “low” gas grid quality of over 90%. The highest CH4 content of 95.626 ± 0.563% is achieved at 55 °C and 4 bar, with a methane formation rate (MFR) of 5.111 ± 0.167 m3/(m3·d). The process performance is highly dependent on the H2:CO2 ratio in the educts, which should be as close as possible to the stochiometric ratio of 4. In conclusion, BHM is a viable approach to upgrade biogas to biomethane quality and can contribute to a sustainable energy grid.
A Selected Review of Impacts of Ocean Deoxygenation on Fish and Fisheries
Authors: Kim Hongsik , Franco Ana C. , Sumaila U. Rashid
Published: June 2023

Oxygen is crucial for the survival of marine species. Yet, the ocean has experienced a loss of approximately 2% of its oxygen inventory since the last century, resulting in adverse impacts on marine life and ecosystems. In particular, changes in the gap between the supply and demand for dissolved oxygen lead to physiological and ecological variations, which cause alterations in habitats and food webs for fish and ecosystem services. These changes vary over time and by region, and the heterogeneous characteristics of marine species bring about non-linear consequences to human society. Despite this, identifying the potential ripple effects of deoxygenation on human society is challenging due to the integrated impacts of other stressors, such as global warming and ocean acidification, and their varying changes depending on environmental conditions and regions, such as upwelling and eutrophication. Therefore, we conducted a literature review on ocean deoxygenation and its effects on fish dynamics and the ecosystem, with a focus on the environmental and societal impact, to present crucial considerations and pathways for future research on ocean deoxygenation. We found that quantitative approaches are necessary to assess the dynamic changes under deoxygenation, and the consequent effects on marine ecosystems should be verified to exploit the natural resources from the ocean. One of the most reliable approaches to quantifying the ripple impacts of deoxygenation is to model spatial and temporal changes with other climate stressors, forming a global network encompassing socio-economic and regional effects of this global change to facilitate and improve capabilities to address the impacts of ocean deoxygenation.
The GRAS Salts of Na2SiO3 and EDTA-Na2 Control Citrus Postharvest Pathogens by Disrupting the Cell Membrane
Authors: Zhao Juan , Wang Yuqing , Liu Qianyi , Liu Shuqi , Pan Hui , Cheng Yunjiang , Long Chaoan
Published: June 2023

Sodium silicate (Na2SiO3) and ethylenediaminetetraacetic acid disodium salt (EDTA-Na2) are inorganic salts classified as ‘Generally Recognized as Safe’ (GRAS) compounds with great advantages in controlling various pathogens of postharvest fruits and vegetables. Here, we determined the median effective concentration (EC50) of Na2SiO3 (0.06%, 0.05%, 0.07% and 0.08%) and EDTA-Na2 (0.11%, 0.08%, 0.5%, and 0.07%) against common pathogens affecting postharvest citrus fruit, including Penicillium digitatum, Penicillium italicum, Geotrichum citri-aurantii, and Colletotrichum gloeosporioides. Na2SiO3 and EDTA-Na2 treatments at the EC50 decreased the spore germination rate, visibly disrupted the spore cell membrane integrity, and significantly increased the lipid droplets (LDs) of the four postharvest pathogens. Moreover, both treatments at EC50 significantly reduced the disease incidence of P. italicum (by 60% and 93.335, respectively) and G. citri-aurantii (by 50% and 76.67%, respectively) relative to the control. Furthermore, Na2SiO3 and EDTA-Na2 treatment resulted in dramatically lower disease severity of the four pathogens, while also demonstrating no significant change in citrus fruit quality compared with the control. Therefore, Na2SiO3 and EDTA-Na2 present a promising approach to control the postharvest diseases of citrus fruit.
Quantitative Detection of Viable but Nonculturable Vibrio parahaemolyticus in Frozen Bivalve Molluscs
Authors: Di Salvo Eleonora , Panebianco Felice , Panebianco Antonio , Ziino Graziella
Published: June 2023

Vibrio parahaemolyticus is a foodborne pathogen diffusely distributed in the marine environment and often isolated from raw seafood belonging to different species, mostly shellfish. Ingestion of under- or uncooked seafood contaminated by V. parahaemolyticus can cause severe gastrointestinal symptoms in humans. Due to its ability to withstand low temperatures, Vibrio spp. could survive in frozen seafoods for long periods by entering the viable but nonculturable state (VBNC) and may constitute an unrecognized source of food contamination and infection. In the present study, seventy-seven frozen bivalve molluscs (35 mussels; 42 clams) were subjected to the detection and enumeration of viable V. parahaemolyticus using standard culture methods. VBNC forms were detected and quantified by applying an optimized protocol based on Propidium Monoazide (PMA) and Quantitative PCR (qPCR). All samples were negative for both the detection and enumeration of V. parahaemolyticus by the standard culture methods. VBNC forms were detected in 11.7% of the samples (9/77), with values ranging from 1.67 to 2.29 Log CFU/g. Only clam samples were positive for the detection of VBNC forms. The results of this study highlighted that VBNC V. parahaemolyticus may be present in frozen bivalve molluscs. Further data on the prevalence of VBNC V. parahaemolyticus in frozen seafood are needed in order to perform a robust risk assessment.
Partial Characterization and Immunomodulatory Effects of Exopolysaccharides from Streptococcus thermophilus SBC8781 during Soy Milk and Cow Milk Fermentation
Authors: Nakata Hajime , Imamura Yoshiya , Saha Sudeb , Lobo René Emanuel , Kitahara Shugo , Araki Shota , Tomokiyo Mikado , Namai Fu , Hiramitsu Masanori , Inoue Takashi , Nishiyama Keita , Villena Julio , Kitazawa Haruki
Published: June 2023

The immunomodulatory properties of exopolysaccharides (EPSs) produced by Streptococcus thermophilus have not been explored in depth. In addition, there are no comparative studies of the functional properties of EPSs produced by streptococci in different food matrices. In this work, EPSs from S. thermophilus SBC8781 were isolated after soy milk (EPS-s) or cow milk (EPS-m) fermentation, identified, and characterized in their abilities to modulate immunity in porcine intestinal epithelial cells. Fresh soy milk and cow milk were inoculated with S. thermophilus SBC8781 (7 log CFU/mL) and incubated at 37 °C for 24 h. The extraction of EPSs was performed by the ethanol precipitation method. Analytical techniques, including NMR, UV-vis spectroscopy, and chromatography, identified and characterized both biopolymer samples as polysaccharides with high purity levels and similar Mw. EPS-s and EPS-m had heteropolysaccharide structures formed by galactose, glucose, rhamnose, ribose, and mannose, although with different monomer proportions. On the other hand, EPS-s had higher quantities of acidic polymer than EPS-m. The biopolymer production of the SBC8781 strain from the vegetable culture broth was 200–240 mg/L, which was higher than that produced in milk, which reached concentrations of 50–70 mg/L. For immunomodulatory assays, intestinal epithelial cells were stimulated with 100 µg/mL of EPS-s or EPS-m for 48 h and then stimulated with the Toll-like receptor 3 agonist poly(I:C). EPS-s significantly reduced the expression of IL-6, IFN-β, IL-8, and MCP-1 and increased the negative regulator A20 in intestinal epithelial cells. Similarly, EPS-m induced a significant reduction of IL-6 and IL-8 expressions, but its effect was less remarkable than that caused by EPS-s. Results indicate that the structure and the immunomodulatory activity of EPSs produced by the SBC8781 strain vary according to the fermentation substrate. Soy milk fermented with S. thermophilus SBC8781 could be a new immunomodulatory functional food, which should be further evaluated in preclinical trials.
Thermal Conductivity of Fractal-Textured Foamed Concrete
Authors: Xiang Guosheng , Li Huajian , Zhou Yinkang , Huang Zhe
Published: June 2023

To provide scientific guidance for the use of foamed concrete (FC) in construction engineering, a thermal conductivity calculation method, based on the fractal model of FC, has been developed. The thermal conductivity (TC) of FC has been tested by the transient planar heat source method in order to verify the reliability of the proposed calculation model. The FC was made of cement, fly ash, and ore powder, and cured under natural conditions for 7 d, 14 d, 28 d, and 42 d, respectively. The TC of FC gradually decreases with the increase in age. The fractal dimension of FC can be determined by both the box-counting method and compressive strength test, and the dimensions determined by both methods are similar. The TC of FC at different porosities and curing ages can be calculated by the fractal dimension, and the estimated values are basically consistent with the test data.
The Effect of Kinesio Taping on Balance and Dynamic Stability in College-Age Recreational Runners with Ankle Instability
Authors: Hussein Hisham M. , Kamel Walid M. , Kamel Ehab M. , Attyia Mohamed R. , Acar Tolgahan , Kanwal Raheela , Ibrahim Ahmed A.
Published: June 2023

(1) Background: Running is one of many sports that have increased in popularity since it can be conducted at any time or anywhere. Ankle instability is a common injury that usually occurs during running and is usually associated with abnormalities in postural stability. Recently, kinesio taping has gained increasing interest as a tool that can be used in rehabilitation, to improve stability, and to help in injury prevention. This study aimed to investigate the effect of Kinesio taping on balance and dynamic stability in recreational runners with ankle instability. (2) Methods: This randomized controlled trial recruited 90 RRs with ankle instability. The participants were randomly divided into three equal groups: a KT group (KTG) who received Kinesio taping on their ankle joints; a mixed group (MG) who received Kinesio taping and exercises; and an exercise group (EG) who received exercises only. Outcome measures (balance and dynamic stability) were assessed before and after the end of an 8-week treatment program using a Biodex balance system and a star excursion balance test, respectively. (3) Results: Within-group comparisons showed statistically significant improvements in most of the outcome values when compared to baseline. Overall stability index was statistically significantly better (with a high effect size) in the MG compared to KTG or EG (p = 0.01, Cohen’s d = 1.6, and p < 0.001, Cohen’s d = 1.63, respectively). A similar finding was evident in the anteroposterior stability index (p = 0.02, Cohen’s d = 0.95, and p < 0.001, Cohen’s d = 1.22, respectively). The mediolateral stability index of the KTG was statistically significantly better with a high effect size when compared to MG or EG (p = 0.04, Cohen’s d = 0.6, and p < 0.01, Cohen’s d = 0.96, respectively). The star excursion balance test values were statistically significant with high effect sizes in the posterior (p = 0.002, Cohen’s d = 1.2) and lateral (p < 0.02, Cohen’s d = 0.92) directions in the MG compared to KTG and EG. (4) Conclusions: Kinesiotape with exercises is superior to either kinesiotape alone or exercises alone in improving postural stability indices and dynamic stability in recreational runners with ankle instability. Recreational runners with ankle instability should be educated about practicing balance exercises and applying kinesiotape.
Use of Infrared Thermometry to Observe Temperature Variation Associated with the Healing Process in Wounds and Ulcers: TIHUAP Cohort Study Protocol
Authors: Iruela Sánchez Mercè , García-Sierra Rosa , Medrano-Jiménez Rafael , Bonachela-Mompart Diana , Maella-Rius Natalia , Soria-Martín Esther , Isnard-Blanchar Mar , Torán-Monserrat Pere
Published: June 2023

We are interested in observing how temperature differences between the wound bed and perilesional skin are related to the healing process in primary care patients with wounds. Multisite prospective cohort study with one-year follow-up in the Metropolitan North area of Barcelona. Recruitment of patients over 18 years with an open wound will take place from January 2023 to September 2023. Temperature checks will be conducted on a weekly basis at control visits and wound care. The following variables will be measured: Percentage reduction of wound area over time, thermal index, the Kundin Wound Gauge, and the Resvech 2.0 Scale. The temperature will be measured weekly using a handheld thermometer and mesh grid to frame the temperature points. The healing trajectory will also be monitored on a monthly basis via photographic imaging, the Resvech Scale, calculation of wound size, percentage reduction of wound area over time, and thermal index for one year of follow-up or until the wound is cured. This study may represent a turning point for its introduction into primary care. Early diagnosis of wound complications would facilitate treatment decision-making for healthcare professionals, thus improving the management of resources related to chronic wounds.
Lower Limb Anthropometric Profiling in Professional Female Soccer Players: A Proof of Concept for Asymmetry Assessment Using Video Analysis
Authors: Weaver Kristian J. , Relph Nicola
Published: June 2023

The objective was to evaluate the clinical joint and limb measures in professional female soccer players. The study was a cross-sectional observational design. It was a preseason clinical setting. The inclusion criteria were outfield professional female soccer players, based in the UK, competing in the highest English league. The exclusion criteria included players who had had surgery in the last six months or had missed a single training session or match due to injury in the previous three months. In terms of the outcome measures, the dependent variables were the true limb length, ankle dorsiflexion, knee flexion and extension, hip flexion, extension, internal rotation and external rotation, and straight leg raise measured using video analysis software. Additionally, passive clinical knee and ankle stability tests were conducted. The independent variables were leg dominance and playing position (defender, midfielder, and attacker). For the results, all the ROM measurements demonstrated limb symmetry (p = 0.621). However, there were significant main effects of the playing position on the ankle dorsiflexion and hip internal rotation, with defenders demonstrating a significantly reduced range of motion in comparison to midfielders and attackers. A notable finding from the bilateral passive stability measures was that 38.3% of players exhibited ankle talar inversion instability when using a talar tilt. In conclusion, bilateral differences do not appear to be apparent in this population; however, positional differences may occur in the ankle and hip range of motion measures. A high proportion of this population may present with passive ankle inversion instability. Future research should consider whether this leads to a higher risk of injury in this population.
The Role and Impact of the Use of Information Technologies by Police in Response to Violence against Women
Authors: Przeszlowski Kimberly , Guerette Rob T. , Sudderth Lori K.
Published: June 2023

The last decade has witnessed an increased awareness of the crucial need to enhance police response and investigation of crimes related to violence against women (VAW). Although some research has been conducted on police decision-making in response to these crimes, there remains a dearth of knowledge concerning the influence of innovative police technologies on the investigative process and resulting case outcomes. This knowledge gap is particularly concerning given (1) the intricate nature and severity of VAW crimes and (2) the substantial advancements in technology that have transformed how the criminal justice system handles violent crime cases. To address this gap, the current study adopted a multi-method, quasi-experimental design to assess the impact of the Miami Police Department’s Real-Time Crime Center (MRTCC) technologies on the case processing and case clearance of sexual assault and domestic violence incidents. The results of this study illuminate the distinctive features associated with this form of violent crime and underscore the necessity of continuously advancing the strategies employed to address these incidents.
The Many Faces of MLKL, the Executor of Necroptosis
Authors: Martinez-Osorio Veronica , Abdelwahab Yasmin , Ros Uris
Published: June 2023

Necroptosis is a recently discovered form of regulated cell death characterized by the disruption of plasma membrane integrity and the release of intracellular content. Mixed lineage kinase domain-like (MLKL) protein is the main player of this cell death pathway as it mediates the final step of plasma membrane permeabilization. Despite the significant progress in our knowledge of the necroptotic pathway and MLKL biology, the precise mechanism of how MLKL functions remain unclear. To understand in what way MLKL executes necroptosis, it is crucial to decipher how the molecular machinery of regulated cell death is activated in response to different stimuli or stressors. It is also indispensable to unveiling the structural elements of MLKL and the cellular players that are required for its regulation. In this review, we discuss the key steps that lead to MLKL activation, possible models that explain how it becomes the death executor in necroptosis, and its emerging alternative functions. We also summarize the current knowledge about the role of MLKL in human disease and provide an overview of existing strategies aimed at developing new inhibitors that target MLKL for necroptosis intervention.
The Effects of Mitochondrial Transplantation on Sepsis Depend on the Type of Cell from Which They Are Isolated
Authors: Kim Yun-Seok , Lee Han A Reum , Lee Min Ji , Park Ye Jin , Mun Sehwan , Yune Chang June , Chung Tae Nyoung , Bae Jinkun , Kim Mi Jin , Choi Yong-Soo , Kim Kyuseok
Published: June 2023

Previously, we have shown that mitochondrial transplantation in the sepsis model has immune modulatory effects. The mitochondrial function could have different characteristics dependent on cell types. Here, we investigated whether the effects of mitochondrial transplantation on the sepsis model could be different depending on the cell type, from which mitochondria were isolated. We isolated mitochondria from L6 muscle cells, clone 9 liver cells and mesenchymal stem cells (MSC). We tested the effects of mitochondrial transplantation using in vitro and in vivo sepsis models. We used the LPS stimulation of THP-1 cell, a monocyte cell line, as an in vitro model. First, we observed changes in mitochondrial function in the mitochondria-transplanted cells. Second, we compared the anti-inflammatory effects of mitochondrial transplantation. Third, we investigated the immune-enhancing effects using the endotoxin tolerance model. In the in vivo polymicrobial fecal slurry sepsis model, we examined the survival and biochemical effects of each type of mitochondrial transplantation. In the in vitro LPS model, mitochondrial transplantation with each cell type improved mitochondrial function, as measured by oxygen consumption. Among the three cell types, L6-mitochondrial transplantation significantly enhanced mitochondrial function. Mitochondrial transplantation with each cell type reduced hyper-inflammation in the acute phase of in vitro LPS model. It also enhanced immune function during the late immune suppression phase, as shown by endotoxin tolerance. These functions were not significantly different between the three cell types of origin for mitochondrial transplantation. However, only L6-mitochondrial transplantation significantly improved survival compared to the control in the polymicrobial intraabdominal sepsis model. The effects of mitochondria transplantation on both in vitro and in vivo sepsis models differed depending on the cell types of origin for mitochondria. L6-mitochondrial transplantation might be more beneficial in the sepsis model.
Genome-Wide Analysis of Strictosidine Synthase-like Gene Family Revealed Their Response to Biotic/Abiotic Stress in Poplar
Authors: Wang Ruiqi , Zhao Wenna , Yao Wenjing , Wang Yuting , Jiang Tingbo , Liu Huanzhen
Published: June 2023

The strictosidine synthase-like (SSL) gene family is a small plant immune-regulated gene family that plays a critical role in plant resistance to biotic/abiotic stresses. To date, very little has been reported on the SSL gene in plants. In this study, a total of thirteen SSLs genes were identified from poplar, and these were classified into four subgroups based on multiple sequence alignment and phylogenetic tree analysis, and members of the same subgroup were found to have similar gene structures and motifs. The results of the collinearity analysis showed that poplar SSLs had more collinear genes in the woody plants Salix purpurea and Eucalyptus grandis. The promoter analysis revealed that the promoter region of PtrSSLs contains a large number of biotic/abiotic stress response elements. Subsequently, we examined the expression patterns of PtrSSLs following drought, salt, and leaf blight stress, using RT-qPCR to validate the response of PtrSSLs to biotic/abiotic stresses. In addition, the prediction of transcription factor (TF) regulatory networks identified several TFs, such as ATMYB46, ATMYB15, AGL20, STOP1, ATWRKY65, and so on, that may be induced in the expression of PtrSSLs in response to adversity stress. In conclusion, this study provides a solid basis for a functional analysis of the SSL gene family in response to biotic/abiotic stresses in poplar.
Targeting MET in Non-Small Cell Lung Cancer (NSCLC): A New Old Story?
Authors: Spagnolo Calogera Claudia , Ciappina Giuliana , Giovannetti Elisa , Squeri Andrea , Granata Barbara , Lazzari Chiara , Pretelli Giulia , Pasello Giulia , Santarpia Mariacarmela
Published: June 2023

In recent years, we have seen the development and approval for clinical use of an increasing number of therapeutic agents against actionable oncogenic drivers in metastatic non-small cell lung cancer (NSCLC). Among them, selective inhibitors, including tyrosine kinase inhibitors (TKIs) and monoclonal antibodies targeting the mesenchymal–epithelial transition (MET) receptor, have been studied in patients with advanced NSCLC with MET deregulation, primarily due to exon 14 skipping mutations or MET amplification. Some MET TKIs, including capmatinib and tepotinib, have proven to be highly effective in this molecularly defined subgroup of patients and are already approved for clinical use. Other similar agents are being tested in early-stage clinical trials with promising antitumor activity. The purpose of this review is to provide an overview of MET signaling pathways, MET oncogenic alterations primarily focusing on exon 14 skipping mutations, and the laboratory techniques used to detect MET alterations. Furthermore, we will summarize the currently available clinical data and ongoing studies on MET inhibitors, as well as the mechanisms of resistance to MET TKIs and new potential strategies, including combinatorial approaches, to improve the clinical outcomes of MET exon 14-altered NSCLC patients.
Microstructured Polymeric Fabrics Modulating the Paracrine Activity of Adipose-Derived Stem Cells
Authors: Grilli Federica , Albanesi Ennio , Pelacho Beatriz , Prosper Felipe , Decuzzi Paolo , Di Mascolo Daniele
Published: June 2023

The deposition of stem cells at sites of injury is a clinically relevant approach to facilitate tissue repair and angiogenesis. However, insufficient cell engraftment and survival require the engineering of novel scaffolds. Here, a regular network of microscopic poly(lactic-co-glycolic acid) (PLGA) filaments was investigated as a promising biodegradable scaffold for human Adipose-Derived Stem Cell (hADSC) tissue integration. Via soft lithography, three different microstructured fabrics were realized where 5 × 5 and 5 × 3 μm PLGA ‘warp’ and ‘weft’ filaments crossed perpendicularly with pitch distances of 5, 10 and 20 μm. After hADSC seeding, cell viability, actin cytoskeleton, spatial organization and the secretome were characterized and compared to conventional substrates, including collagen layers. On the PLGA fabric, hADSC re-assembled to form spheroidal-like structures, preserving cell viability and favoring a nonlinear actin organization. Moreover, the secretion of specific factors involved in angiogenesis, the remodeling of the extracellular matrix and stem cell homing was favored on the PLGA fabric as compared to that which occurred on conventional substrates. The paracrine activity of hADSC was microstructure-dependent, with 5 μm PLGA fabric enhancing the expression of factors involved in all three processes. Although more studies are needed, the proposed PLGA fabric would represent a promising alternative to conventional collagen substrates for stem cell implantation and angiogenesis induction.
Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach
Authors: Monleón-Guinot Irene , Milian Lara , Martínez-Vallejo Patricia , Sancho-Tello María , Llop-Miguel Mauro , Galbis José Marcelo , Cremades Antonio , Carda Carmen , Mata Manuel
Published: June 2023

The malignity of lung cancer is conditioned by the tumor microenvironment (TME), in which cancer-associated fibroblasts (CAFs) are relevant. In this work, we generated organoids by combining A549 cells with CAFs and normal fibroblasts (NF) isolated from adenocarcinoma tumors. We optimized the conditions for their manufacture in a short time. We evaluated the morphology of organoids using confocal microscopy analysis of F-actin, vimentin and pankeratin. We determined the ultrastructure of the cells in the organoids via transmission electron microscopy and the expression of CDH1, CDH2 and VIM via RT-PCR. The addition of stromal cells induces the self-organization of the organoids, which acquired a bowl morphology, as well as their growth and the generation of cell processes. They also influenced the expression of genes related to epithelial mesenchymal transition (EMT). CAFs potentiated these changes. All cells acquired a characteristic secretory phenotype, with cohesive cells appearing inside the organoids. In the periphery, many cells acquired a migratory phenotype, especially in organoids that incorporated CAFs. The deposit of abundant extracellular matrix could also be observed. The results presented here reinforce the role of CAFs in the progression of lung tumors and could lay the foundation for a useful in vitro pharmacological model.
Molecular Mechanisms and Risk Factors Related to the Pathogenesis of Peyronie’s Disease
Authors: Mitsui Yozo , Yamabe Fumito , Hori Shunsuke , Uetani Masato , Kobayashi Hideyuki , Nagao Koichi , Nakajima Koichi
Published: June 2023

Peyronie’s disease (PD) is a benign condition caused by plaque formation on the tunica albuginea of the penis. It is associated with penile pain, curvature, and shortening, and contributes to erectile dysfunction, which worsens patient quality of life. In recent years, research into understanding of the detailed mechanisms and risk factors involved in the development of PD has been increasing. In this review, the pathological mechanisms and several closely related signaling pathways, including TGF-β, WNT/β-catenin, Hedgehog, YAP/TAZ, MAPK, ROCK, and PI3K/AKT, are described. Findings regarding cross-talk among these pathways are then discussed to elucidate the complicated cascade behind tunica albuginea fibrosis. Finally, various risk factors including the genes involved in the development of PD are presented and their association with the disease summarized. The purpose of this review is to provide a better understanding regarding the involvement of risk factors in the molecular mechanisms associated with PD pathogenesis, as well as to provide insight into disease prevention and novel therapeutic interventions.
Disruptions of Circadian Genes in Cutaneous Melanoma—An In Silico Analysis of Transcriptome Databases
Authors: Lesicka Monika , Nedoszytko Bogusław , Reszka Edyta
Published: June 2023

Circadian genes are a set of genes that regulate the body’s internal clock and influence various physiological processes, including sleep–wake cycles, metabolism and immune function. Skin cutaneous melanoma (SKCM) is a type of skin cancer that arises from the pigment-producing cells in the skin and is the most deadly form of skin cancer. This study has investigated the relevance of circadian gene expression and immune infiltrations in the outcomes of cutaneous melanoma patients. In the present study, in silico methods based on the GEPIa, TIMER 2.0 and cBioPortal databases were performed, so as to investigate the transcript level and prognostic value of 24 circadian genes in SKCM and their relationship with the immune infiltration level. The in silico analysis showed that significantly more than half of the investigated circadian genes have an altered transcript pattern in cutaneous melanoma compared to normal skin. The mRNA levels of TIMELES and BHLHE41 were upregulated, whereas those of NFIL3, BMAL1, HLF, TEF, RORA, RORC, NR1D1, PER1, PER2, PER3, CRY2 and BHLHE40 were downregulated. The presented research shows that SKCM patients with at least one alteration of their circadian genes have decreased overall survival. Additionally, majority of the circadian genes are significantly corelated with the immune cells’ infiltration level. The strongest correlation was found for neutrophils and was followed by circadian genes: NR1D2 r = 0.52 p < 0.0001, BMAL1 r = 0.509 p < 0.0001; CLOCK r = 0.45 p < 0.0001; CSNKA1A1 r = 0.45 p < 0.0001; RORA r = 0.44 p < 0.0001. The infiltration level of immune cells in skin tumors has been associated with patient prognosis and treatment response. Circadian regulation of immune cell infiltration may further contribute to these prognostic and predictive markers. Examining the correlation between circadian rhythm and immune cell infiltration can provide valuable insights into disease progression and guide personalized treatment decisions.
Serum Oxytocin Levels Decrease 12 Months Following Sleeve Gastrectomy and Are Associated with Decreases in Lean Mass
Authors: Becetti Imen , Singhal Vibha , Nimmala Supritha , Lee Hang , Lawson Elizabeth A. , Bredella Miriam A. , Misra Madhusmita
Published: June 2023

Oxytocin (OXT), an anorexigenic hormone, is also bone anabolic. Further, OXT administration results in increases in lean mass (LM) in adults with sarcopenic obesity. We examine, for the first time, associations of OXT with body composition and bone endpoints in 25 youth 13–25 years old with severe obesity who underwent sleeve gastrectomy (SG) and 27 non-surgical controls (NS). Forty participants were female. Subjects underwent fasting blood tests for serum OXT and DXA for areal bone mineral density (aBMD) and body composition. At baseline, SG vs. NS had higher median body mass index (BMI) but did not differ for age or OXT levels. Over 12 months, SG vs. NS had greater reductions in BMI, LM, and fat mass (FM). OXT decreased in SG vs. NS 12 months post-SG. While baseline OXT predicted a 12-month BMI change in SG, decreases in OXT levels 12 months post-SG were not associated with decreases in weight or BMI. In SG, decreases in OXT were positively associated with decreases in LM but not with decreases in FM or aBMD. Loss of LM, a strong predictor of BMD, after bariatric surgery may reduce functional and muscular capacity. OXT pathways may be targeted to prevent LM loss following SG.
Investigating the Physiological and Molecular Responses of Solanum lycopersicum hp Mutants to Light of Different Quality for Biotechnological Applications
Authors: Vereshchagin Mikhail , Kreslavski Vladimir , Ivanov Yury , Ivanova Alexandra , Kumachova Tamara , Ryabchenko Andrey , Kosobryukhov Anatoliy , Kuznetsov Vladimir , Pashkovskiy Pavel
Published: June 2023

The effect of the light of different spectral compositions, white fluorescent light (WFL), red light (RL, 660 nm), blue light (BL, 450 nm), green light (GL, 525 nm), and white LED light (WL, 450 + 580 nm), on the physiological parameters of Solanum lycopersicum 3005 hp-2 (defective for a DET1 gene) and 4012 hp-1w; 3538 hp-1; 0279 hp-1.2 (defective for a DDB1a gene) photomorphogenetic mutants was studied. The parameters of the primary photochemical processes of photosynthesis, photosynthetic and transpiration rates, the antioxidant capacity of low-molecular weight antioxidants, the content of the total phenolic compounds, including flavonoids, and the expression of the genes involved in light signaling and biosynthesis of secondary metabolites were determined. Under BL, the 3005 hp-2 mutant showed the highest nonenzymatic antioxidant activity, which occurred to a greater extent due to the increase in flavonoid content. At the same time, under BL, the number of secretory trichomes on the surface of the leaves of all mutants increased equally. This suggests the accumulation of flavonoids inside leaf cells rather than in trichomes on the leaf surface. The data obtained indicate the possibility of using the hp-2 mutant for biotechnology to increase its nutritional value by enhancing the content of flavonoids and other antioxidants by modulating the spectral composition of light.
Analysis of CircRNA Expression in Peripheral Blood of Holstein Cows in Response to Heat Stress
Authors: Zhang Congcong , Wang Shuhui , Hu Lirong , Fang Hao , Chen Gong , Ma Xiaojuan , Yu Ying , Wang Yachun , Xu Qing
Published: June 2023

The present study aimed to identify key circRNAs and pathways associated with heat stress in blood samples of Holstein cows, which will provide new insights into the molecular mechanisms driving heat stress in cows. Hence, we evaluated changes in milk yield, rectal temperature, and respiratory rate of experimental cows between heat stress (summer) and non-heat stress (spring) conditions with two comparisons, including Sum1 vs. Spr1 (same lactation stage, different individuals, 15 cows per group) and Sum1 vs. Spr2 (same individual, different lactation stages, 15 cows per group). Compared to both Spr1 and Spr2, cows in the Sum1 group had a significantly lower milk yield, while rectal temperature and respiratory rate were significantly higher (p < 0.05), indicating that cows in the Sum1 group were experiencing heat stress. In each group, five animals were chosen randomly to undergo RNA-seq. The results reveal that 140 and 205 differentially expressed (DE) circRNAs were screened in the first and second comparisons, respectively. According to the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, these DE circRNAs were mainly enriched in five signaling pathways, including choline metabolism, the PI3K/AKT signaling pathway, the HIF-1 signaling pathway, the longevity-regulating pathway, and autophagy. Then, we obtained the top 10 hub source genes of circRNAs according to the protein–protein interaction networks. Among them, ciRNA1282 (HIF1A), circRNA4205 (NR3C1), and circRNA12923 (ROCK1) were enriched in multiple pathways and identified as binding multiple miRNAs. These key circRNAs may play an important role in the heat stress responses of dairy cows. These results provide valuable information on the involvement of key circRNAs and their expression pattern in the heat stress response of cows.
Left Atrial Strain Imaging by Speckle Tracking Echocardiography: The Supportive Diagnostic Value in Cardiac Amyloidosis and Hypertrophic Cardiomyopathy
Authors: Monte Ines Paola , Faro Denise Cristiana , Trimarchi Giancarlo , de Gaetano Fabrizio , Campisi Mariapaola , Losi Valentina , Teresi Lucio , Di Bella Gianluca , Tamburino Corrado , de Gregorio Cesare
Published: June 2023

Background: Left atrial (LA) function is crucial for assessing left ventricular filling in various cardiovascular conditions. Cardiac Amyloidosis (CA) is characterized by atrial myopathy and LA function impairment, with diastolic dysfunction up to restrictive filling pattern, leading to progressive heart failure and arrhythmias. This study evaluates LA function and deformation using speckle tracking echocardiography (STE) in patients with CA compared to a cohort of patients with sarcomeric Hypertrophic Cardiomyopathy (HCM) and a control group. Methods: We conducted a retrospective, observational study (from January 2019 to December 2022) including a total of 100 patients: 33 with ATTR-CA, 34 with HCMs, and 33 controls. Clinical evaluation, electrocardiograms, and transthoracic echocardiography were performed. Echocardiogram images were analyzed in post-processing using EchoPac software for LA strain quantification, including LA-reservoir, LA-conduit, and LA-contraction strain. Results: The CA group exhibited significantly impaired LA function compared to HCMs and control groups, with LA-reservoir median values of −9%, LA-conduit −6.7%, and LA-contraction −3%; this impairment was consistent even in the CA subgroup with preserved ejection fraction. LA strain parameters correlated with LV mass index, LA volume index, E/e’, and LV-global longitudinal strain and were found to be associated with atrial fibrillation and exertional dyspnea. Conclusions: LA function assessed by STE is significantly impaired in CA patients compared to HCMs patients and healthy controls. These findings highlight the potential supportive role of STE in the early detection and management of the disease.
Evaluation of the Effectiveness of a Nordic Walking and a Resistance Indoor Training Program: Anthropometric, Body Composition, and Functional Parameters in the Middle-Aged Population
Authors: Grigoletto Alessia , Mauro Mario , Toselli Stefania
Published: June 2023

Sedentary behaviors are increasing in the population, so strategies for the increment of physical activity levels are needed. The use of green space seems to be a valid support to be more active. The present study aimed to compare the effectiveness of a period of outdoor training (Nordic walking (NW)) with indoor resistance training (GYM) in a nonclinical population based on anthropometric characteristics, body composition, and functional parameters. This study was conducted on 102 participants (77 middle-aged people performed NW and 25 performed indoor training). Participants were measured twice: at baseline and after three months. Anthropometric measurements (weight, BMI, skinfolds, perimeters), body composition, bioelectrical impedance, vectorial analysis (BIA and BIVA), and physical tests were carried out. A two-way repeated measures analysis of variance (ANOVA) was performed to evaluate the effect of the treatments, groups, and sexes. There were several intervention effects linked to a decrease in fat parameters (such as skinfolds, fat mass, and percentage of fat mass). Considering the type of intervention, NW showed a higher increase in muscle mass and a higher decrease in fat parameters than the GYM group. In conclusion, the two types of training could represent a good way to remain active and prevent sedentary behaviors.
Contributions of Lower Structures to Higher Cognition: Towards a Dynamic Network Model
Authors: Saban William , Gabay Shai
Published: June 2023

Researchers often attribute higher cognition to the enlargement of cortical regions throughout evolution, reflecting the belief that humans sit at the top of the cognitive pyramid. Implicitly, this approach assumes that the subcortex is of secondary importance for higher-order cognition. While it is now recognized that subcortical regions can be involved in various cognitive domains, it remains unclear how they contribute to computations essential for higher-level cognitive processes such as endogenous attention and numerical cognition. Herein, we identify three models of subcortical–cortical relations in these cognitive processes: (i) subcortical regions are not involved in higher cognition; (ii) subcortical computations support elemental forms of higher cognition mainly in species without a developed cortex; and (iii) higher cognition depends on a whole-brain dynamic network, requiring integrated cortical and subcortical computations. Based on evolutionary theories and recent data, we propose the SEED hypothesis: the Subcortex is Essential for the Early Development of higher cognition. According to the five principles of the SEED hypothesis, subcortical computations are essential for the emergence of cognitive abilities that enable organisms to adapt to an ever-changing environment. We examine the implications of the SEED hypothesis from a multidisciplinary perspective to understand how the subcortex contributes to various forms of higher cognition.
Wind Drift, Breakdown, and Pile Up of the Ice Field
Authors: Goncharov Vadim K.
Published: June 2023

This article contains the analytical model of the drift of a separate ice field under the action of wind and current, in which velocities and directions can vary over time. The model takes into account the mass of ice, added mass of seawater, and the effects of the wind and current on the ice field in forming the friction on its upper and underwater surfaces and the frontal resistance on its end (forward and backward) surfaces. Simulation of the wind drift of the ice field showed the drift velocity exceeds the considerable known velocity of a compact ice cover drift. A drifting ice field has a certain kinetic energy that should be released when a collision occurs with an unmovable obstacle, and spent on brittle breakdown of a quantity of the ice field. The volume of formed small ice pieces (fragments of ice field) was estimated by comparison of the specific energy of the sea ice brittle destruction and the kinetic energy of the drifting ice field. The article presents the results of the estimation of the possible volume of the ice pieces and the scales of formed piles as a result of a collision with an obstacle, depending on the initial dimensions of the ice field and wind speed. Developed models and the results of computer modeling can be used to estimate the ice pile sizes near the stationary platforms and terminals on the Arctic seas.
Investigation on the Influence of Production and Incubation Temperature on the Growth, Virulence, Germination, and Conidial Size of Metarhizium brunneum for Granule Development
Authors: Seib Tanja , Fischer Katharina , Sturm Anna Maria , Stephan Dietrich
Published: June 2023

Important for the infection of an insect with an entomopathogenic fungus and its use as a plant protection agent are its growth, conidiation, germination, and virulence, which all depend on the environmental temperature. We investigated not only the effect of environmental temperature but also that of production temperature of the fungus. For this purpose, Metarhizium brunneum JKI-BI-1450 was produced and incubated at different temperatures, and the factors mentioned as well as conidial size were determined. The temperature at which the fungus was produced affects its subsequent growth and conidiation on granule formulation, the speed of germination, and the conidial width, but not its final germination or virulence. The growth and conidiation was at its highest when the fungus was produced at 25 °C, whereas when the germination was faster, the warmer the fungus was produced. The incubation temperature optimum of JKI-BI-1450 in relation to growth, speed of germination, and survival time was 25–30 °C and for conidiation 20–25 °C. Conidial length decreased with increasing incubation temperature. Although the fungus could not be adapted to unfavorable conditions by the production temperature, it was found that the quality of a biological control agent based on entomopathogenic fungi can be positively influenced by its production temperature.