bluuebunny/crossref_metadata_2025 huggingface dataset:
The First Vietnam War
Authors: McHale Shawn F.
Published: August 2021

Shawn McHale explores why the communist-led resistance in Vietnam won the anticolonial war against France (1945–54), except in the south. He shows how broad swaths of Vietnamese people were uneasily united in 1945 under the Viet Minh Resistance banner, all opposing the French attempt to reclaim control of the country. By 1947, resistance unity had shattered and Khmer-Vietnamese ethnic violence had divided the Mekong delta. From this point on, the war in the south turned into an overt civil war wrapped up in a war against France. Based on extensive archival research in four countries and in three languages, this is the first substantive English-language book focused on southern Vietnam's transition from colonialism to independence.
Lithium Isotopes
Authors: Strandmann Philip A. E. Pogge von , Dellinger Mathieu , West A. Joshua
Published: August 2021

Lithium isotopes are a relatively novel tracer of present and past silicate weathering processes. Given that silicate weathering is the primary long-term method by which CO2 is removed from the atmosphere, Li isotope research is going through an exciting phase. We show the weathering processes that fractionate dissolved and sedimentary Li isotope ratios, focusing on weathering intensity and clay formation. We then discuss the carbonate and silicate archive potential of past seawater δ7Li. These archives have been used to examine Li isotope changes across both short and long timescales. The former can demonstrate the rates at which the climate is stabilised from perturbations via weathering, a fundamental piece of the puzzle of the long-term carbon cycle.
Buddhaghosa, James, and Thompson on Conscious Flow
Authors: FORTNEY MARK
Published: August 2021

This paper is about whether consciousness flows. Evan Thompson (2014) has recently claimed that the study of binocular rivalry shows that there are some moments where consciousness does not flow, contra William James (1890). Moreover, he has claimed that Abhidharma philosophers reject James's claim that consciousness flows. I argue that binocular rivalry poses no special challenge to James. Second, I argue that because Thompson did not take up the question of how James and Abhidharma philosophers analyze or define flow, he underdescribed their disagreement in a way that obscures an important conceptual contribution that Abhidharma philosophers make to the study of flow. They reject James's claim that there are only two conceivable ways for consciousness to fail to flow and suggest that there is a third way for consciousness to fail to flow—a way that James's imagination did not reveal to be possible.
Social Network Analysis for Implementation of the Sendai Framework for Disaster Risk Reduction in Iran
Authors: Yousefi Khoshsabegheh Homa , Ardalan Ali , Takian Amirhossein , Hedayatifar Leila , Ostadtaghizadeh Abbas , Saeedi Behnam
Published: August 2021

Background:

Stakeholders are responsible for managing the risks of disasters. Hence, appropriate, collaborative, timely interactions of involved organizations, and having a collective view of these interactions, have an important influence on the operation of the whole system. This study was aimed at social network analysis (SNA) for the implementation of the Sendai framework for disaster risk reduction in Iran.

Methods:

SNA was used in this study. A review of literature on disaster risk management (DRM) plus snowball sampling technique identified a list of 85 stakeholders. Delphi method among purposefully selected experts was used to score the relationship between the stakeholders. Louvain method, along with the modularity optimization method, was applied to identify groups of stakeholders with greater interactions. Centrality measurements were used to define organizations with key-roles in the network.

Results:

The density of this network was 0.75, which showed that not all the stakeholders were connected. The National Disaster Management Organization and Civil Defense Organization showed higher influences considering their responsibilities. A total of 3 clusters of stakeholders with specific duties that mostly interact with each other and have some interaction with other groups were recognized.

Conclusion:

Understanding the pre-disaster interaction of the network and the strengths and weaknesses of the interactions among stakeholders could help improve DRM.
The Evolution of Disaster Risk Management: Historical Approach
Authors: Rajabi Elham , Bazyar Jafar , Delshad Vahid , Khankeh Hamid Reza
Published: August 2021

The concept of Disaster Risk Management (DRM) has changed throughout history. Identifying changes and related factors can be effective in adopting logical, scientific and evidence-based approaches in the future. Therefore, this study was conducted with the aim of depicting the process of changes in the concept of DRM and creating an original perspective. In this narrative literature review study, we used historical approach. Literature, regardless of the time of publication, was searched using divergent keywords including “disaster, health, emergency, management, risk, disaster medicine, and hazard.” DRM evolution started with the emergence of civil defense during the last century. Although DRM was initially focused on responses, currently, this concept includes disaster risk reduction (DRR) and disaster management. DRR includes prevention and mitigation, and disaster management includes response and recovery measures. DRR considering underlying risk factors such as social factors, and focusing on participation of communities are important steps to be taken.
The Swiss Primate Case: How Courts Have Paved the Way for the First Direct Democratic Vote on Animal Rights
Authors: Blattner Charlotte E. , Fasel Raffael
Published: August 2021

A citizens’ initiative was launched in 2016 in the Swiss canton of Basel-Stadt, demanding that the rights catalogue in the Cantonal Constitution be complemented by a fundamental right to life and a right to bodily and mental integrity for non-human primates. This initiative became the subject of a three-year legal dispute that ended with a decision of the Swiss Federal Supreme Court in September 2020, ruling that the initiative is legally valid and must be put to the people for a vote. This case note discusses the key developments in the dispute, including the groundbreaking decision by the Constitutional Court of Basel-Stadt, which held that cantons are free to ‘expand the circle of rights holders beyond the anthropological barrier’. The authors, who were involved in the drafting of the initiative and acted as legal advisers in the judicial proceedings, offer first-hand insights into legal strategies and shed light on the importance of the case in the context of the ongoing efforts to secure rights for primates around the world.
Fuel‐Specific Carbon Footprint Embodied in Japanese Household Lifestyles
Authors: Long Yin , Yoshida Yoshikuni , Zeng Isabella Yunfei , Xue Jinjun , Li Yuan
Published: August 2021

To comprehensively investigate how regional household contribute to national carbon emission, this paper evaluates both the direct and indirect Japanese household carbon‐based emissions by using an environmentally extended input‐output table as well as the regional household consumption inventory. The results indicate that the household sector is the key driving force behind Japan's emissions, and it accounts for approximately 80% of the country's total emissions when indirect emissions are included. Moreover, significant regional differences exist in terms of the carbon footprint, which is in part caused by household and fuel‐type differences found across the country. Finally, this paper suggests that the impact of regional household and fuel‐type differences on Japan's overall carbon footprint should be a primary focus when practitioners design customized strategies to decarbonize Japan at the subnational scale.
Antarctic Slope Current Modulates Ocean Heat Intrusions Towards Totten Glacier
Authors: Nakayama Yoshihiro , Greene Chad A. , Paolo Fernando S. , Mensah Vigan , Zhang Hong , Kashiwase Haruhiko , Simizu Daisuke , Greenbaum Jamin S. , Blankenship Donald D. , Abe‐Ouchi Ayako , Aoki Shigeru
Published: August 2021

The Totten ice shelf (TIS) in East Antarctica has received increasing attention in recent years due to high basal melt rates, which have been linked to a presence of warm modified Circumpolar Deep Water (mCDW) observed at the ice front. We show that mCDW on‐shelf intrusions towards the TIS strengthen when the Antarctic Slope Current (ASC) weakens. This demonstrates that the ASC has a blocking effect and ASC weakening leads to on‐shelf intrusions, as proposed by previous observational studies. The interannual variability of the ASC is controlled primarily by atmospheric and oceanic conditions beyond our regional model domain. We further show that heat intrusions onto the continental shelf off the TIS are not influenced by off‐shelf warming but are enhanced with coastal freshening, suggesting positive feedback whereby ice melt and freshening upstream could start a chain reaction, leading to increased melt, and further coastal freshening.
Detection of “Rapid” Aseismic Slip at the Izu‐Bonin Trench
Authors: Fukao Yoshio , Kubota Tatsuya , Sugioka Hiroko , Ito Aki , Tonegawa Takashi , Shiobara Hajime , Yamashita Mikiya , Saito Tatsuhiko
Published: August 2021

No great earthquakes have been historically documented at the Izu‐Bonin Trench, where subduction is believed to occur largely by aseismic slip, although the details are poorly understood. We deployed an array of ocean bottom pressure gauges here for a year from May 2015. The array recorded the coseismic seafloor uplift/subsidence and tsunamis generated by the nearby Mw6.0 thrust earthquake. In association with this event, we detected two much larger aseismic slip events with rise times around 1 h. The total moment of these two aseismic events was 17 times larger than that of the mainshock. Such aseismic, yet still rapid, slip can be interpreted as one amid the transitional regime. This regime is expected to host slow slip events near its boundary with the stable sliding regime and, possibly, tsunami earthquakes and very low frequency earthquakes near the boundary with the unstable seismic slip regime. Slip in the transitional regime may be a prevalent mode of subduction in the Izu‐Bonin trench, where effective normal force on the frictional plate interface is tectonically reduced.
Stratigraphy and Provenance of the Paleogene Syn‐Rift Sediments in Central‐Southern Palawan: Paleogeographic Significance for the South China Margin
Authors: Chen Wen‐Huang , Yan Yi , Carter Andrew , Huang Chi‐Yue , Yumul Graciano P. , Dimalanta Carla B. , Gabo‐Ratio Jillian Aira S. , Wang Ming‐Huei , Chen Duofu , Shan Yehua , Zhang Xin‐Chang , Liu Weiliang
Published: August 2021

The Palawan microcontinental block is thought to have separated from the South China margin due to seafloor spreading and opening of the South China Sea. However, it is uncertain when and from which section the Palawan microcontinental block rifted from the South China margin and little is known about sediment routing across the rifted margin before continental breakup. To address these aspects, we studied the biostratigraphy and provenance of syn‐rift sedimentary rocks collected from the Panas‐Pandian Formation in central‐southern Palawan. Micropaleontological evidence indicates a Middle Eocene‐earliest Oligocene (47.7–32.9 Ma) age for the Panas‐Pandian Formation. Based on this and the oldest age of the post‐rift Nido Limestone (∼32 Ma), the breakup unconformity on the Palawan microcontinent block is dated around 33–32 Ma. This timing of breakup unconformity is close to that of the Pearl River Mouth Basin (∼30 Ma) and IODP Site U1435 (∼34 Ma), suggesting a conjugate relationship between the Palawan microcontinental block and the Pearl River Mouth Basin. Trace fossils and benthic foraminifera from the Panas‐Pandian Formation indicate a middle bathyal to abyssal environment on the continental slope of the South China margin. Multidisciplinary provenance analysis reveals that the Panas‐Pandian Formation was derived from both local Mesozoic basement uplifts and the interior Cathaysia Block. It indicates that a paleo‐Pearl River has been established at least since the Middle Eocene (47.7–41.9 Ma) and could deliver sediments from the interior Cathaysia Block to the continental slope, across the wide rifted margin with a low topographic gradient.
Implementation of multilingual support of the European Multicenter Study about Spinal Cord Injury (EMSCI) ISNCSCI calculator
Authors: Schuld Christian , Franz Steffen , Schweidler Joachim , Kriz Jiri , Hakova Renata , Weidner Norbert , Rupp Rüdiger , Liu Nan
Published: August 2021

Objectives

Since their introduction, electronic International Standards for Neurological Classification of Spinal Cord Injury (ISNCSCI) calculators have evolved to powerful tools providing error-free ISNCSCI classifications in education, research and clinical practice. For increased accessibility and dissemination, a multilingual support is mandatory. The aim of this work was to setup a general multilingual framework for the freely available ISNCSCI calculator (https://ais.emsci.org) of the European Multicenter Study about Spinal Cord Injury (EMSCI).

Methods

The graphical user interface (GUI) and PDF export of the ISNCSCI worksheet were adapted for multilingual implementations. Their language-dependent content was identified. These two steps called internationalization have to be performed by a programmer in preparation of the translations of the English terms into the target language. This step following the internationalization is called localization and needs input by a bi-lingual clinical expert. Two EMSCI partners provided Standard Mandarin Chinese and Czech translations. Finally, the translations are made available in the application.

Results

The GUI and PDF export of the ISNCSCI worksheet were internationalized. The default language of the calculator is set according to the user’s preferences with the additional possibility for manual language selection. The Chinese as well as a Czech translation were provided freely to the SCI community.

Conclusions

The possibility of multilingual implementations independent from software developers opens the use of ISNCSCI computer algorithms as an efficient training tool on a larger scale.
Repurposing the estrogen receptor modulator raloxifene to treat SARS-CoV-2 infection
Authors: Allegretti Marcello , Cesta Maria Candida , Zippoli Mara , Beccari Andrea , Talarico Carmine , Mantelli Flavio , Bucci Enrico M. , Scorzolini Laura , Nicastri Emanuele
Published: August 2021

The ongoing coronavirus disease 2019 (COVID-19) pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) necessitates strategies to identify prophylactic and therapeutic drug candidates to enter rapid clinical development. This is particularly true, given the uncertainty about the endurance of the immune memory induced by both previous infections or vaccines, and given the fact that the eradication of SARS-CoV-2 might be challenging to reach, given the attack rate of the virus, which would require unusually high protection by a vaccine. Here, we show how raloxifene, a selective estrogen receptor modulator with anti-inflammatory and antiviral properties, emerges as an attractive candidate entering clinical trials to test its efficacy in early-stage treatment COVID-19 patients.
Crystal structure of the INTS3/INTS6 complex reveals the functional importance of INTS3 dimerization in DSB repair
Authors: Jia Yu , Cheng Zixiu , Bharath Sakshibeedu R. , Sun Qiangzu , Su Nannan , Huang Jun , Song Haiwei
Published: August 2021

SOSS1 is a single-stranded DNA (ssDNA)-binding protein complex that plays a critical role in double-strand DNA break (DSB) repair. SOSS1 consists of three subunits: INTS3, SOSSC, and hSSB1, with INTS3 serving as a scaffold to stabilize this complex. Moreover, the integrator complex subunit 6 (INTS6) participates in the DNA damage response through direct binding to INTS3, but how INTS3 interacts with INTS6, thereby impacting DSB repair, is not clear. Here, we determined the crystal structure of the C-terminus of INTS3 (INTS3c) in complex with the C-terminus of INTS6 (INTS6c) at a resolution of 2.4 Å. Structural analysis revealed that two INTS3c subunits dimerize and interact with INTS6c via conserved residues. Subsequent biochemical analyses confirmed that INTS3c forms a stable dimer and INTS3 dimerization is important for recognizing the longer ssDNA. Perturbation of INTS3c dimerization and disruption of the INTS3c/INTS6c interaction impair the DSB repair process. Altogether, these results unravel the underappreciated role of INTS3 dimerization and the molecular basis of INTS3/INTS6 interaction in DSB repair.
Frequency-preference response in covalent modification cycles under substrate sequestration conditions
Authors: Szemere Juliana Reves , Rotstein Horacio G. , Ventura Alejandra C.
Published: August 2021

Covalent modification cycles (CMCs) are basic units of signaling systems and their properties are well understood. However, their behavior has been mostly characterized in situations where the substrate is in excess over the modifying enzymes. Experimental data on protein abundance suggest that the enzymes and their target proteins are present in comparable concentrations, leading to substrate sequestration by the enzymes. In this enzyme-in-excess regime, CMCs have been shown to exhibit signal termination, the ability of the product to return to a stationary value lower than its peak in response to constant stimulation, while this stimulation is still active, with possible implications for the ability of systems to adapt to environmental inputs. We characterize the conditions leading to signal termination in CMCs in the enzyme-in-excess regime. We also demonstrate that this behavior leads to a preferred frequency response (band-pass filters) when the cycle is subjected to periodic stimulation, whereas the literature reports that CMCs investigated so far behave as low-pass filters. We characterize the relationship between signal termination and the preferred frequency response to periodic inputs and we explore the dynamic mechanism underlying these phenomena. Finally, we describe how the behavior of CMCs is reflected in similar types of responses in the cascades of which they are part. Evidence of protein abundance in vivo shows that enzymes and substrates are present in comparable concentrations, thus suggesting that signal termination and frequency-preference response to periodic inputs are also important dynamic features of cell signaling systems, which have been overlooked.
Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
Authors: Venugopal Dilip , Kasani Nanda , Manjunath Yariswamy , Li Guangfu , Kaifi Jussuf T. , Kwon Jae W.
Published: August 2021

Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.
Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide
Authors: Li Yumei , Ye Zhengmao , Lu Peng , Lu Lingchao
Published: August 2021

A soluble melanin pigment produced by Streptomyces sp. ZL-24 was purified and named StrSM. The elemental analysis of StrSM showed it consists of carbon, hydrogen, and oxygen. The spectrum analysis, including ultraviolet–visible absorption spectrum, Fourier-transform infrared spectrum, and pyrolysis–gas chromatography–mass spectrometry, indicated that StrSM might be pyomelanin. High performance liquid chromatography and liquid chromatography–mass spectra analysis of intermediate metabolite showed the presence of homogentisic acid (HGA). Moreover, the enzyme 4-hydroxyphenylpyruvate dioxygenase, involved in HGA biosynthesis, showed high activity during melanin production. Subsequently, a tyrosinase gene (melC2) and hydroxyphenylpyruvate dioxygenase gene double mutant demonstrated StrSM is pyomelanin. In vitro bioactivity assay showed that StrSM had excellent protective capability against SH-SY5Y cell oxidative injury. To our knowledge, the results firstly provide comprehensive data on Streptomyces pyomelanin identification and a promising candidate compound to treat oxidative injury of neurocytes.
Percolation of collagen stress in a random network model of the alveolar wall
Authors: Casey Dylan T. , Bou Jawde Samer , Herrmann Jacob , Mori Vitor , Mahoney J. Matthew , Suki Béla , Bates Jason H. T.
Published: August 2021

Fibrotic diseases are characterized by progressive and often irreversible scarring of connective tissue in various organs, leading to substantial changes in tissue mechanics largely as a result of alterations in collagen structure. This is particularly important in the lung because its bulk modulus is so critical to the volume changes that take place during breathing. Nevertheless, it remains unclear how fibrotic abnormalities in the mechanical properties of pulmonary connective tissue can be linked to the stiffening of its individual collagen fibers. To address this question, we developed a network model of randomly oriented collagen and elastin fibers to represent pulmonary alveolar wall tissue. We show that the stress–strain behavior of this model arises via the interactions of collagen and elastin fiber networks and is critically dependent on the relative fiber stiffnesses of the individual collagen and elastin fibers themselves. We also show that the progression from linear to nonlinear stress–strain behavior of the model is associated with the percolation of stress across the collagen fiber network, but that the location of the percolation threshold is influenced by the waviness of collagen fibers.
Adaptive and maladaptive features of schizotypy clusters in a community sample
Authors: Polner Bertalan , Hupuczi Ernő , Kéri Szabolcs , Kállai János
Published: August 2021

Schizotypal personality traits correlate with psychopathology and impaired functional outcome. Yet advantageous aspects of positive schizotypy may exist which could promote resilience and creativity, and several studies have identified a high positive but low negative schizotypy group with some signs of adaptation. The aim of our study was to clarify whether such individuals demonstrate only traits associated with well-being, or they also have traits that predict impairment. Participants (N = 643 students, 71.5% female) completed measures of schizotypy, resilience, self-esteem, self-concept clarity, and absorption. We identified four clusters: an overall low schizotypy, an overall high schizotypy, a disorganised-interpersonal schizotypy and a positive schizotypy cluster. The overall high schizotypy cluster seemed to be the most vulnerable as it was the least resilient and showed widespread maladaptation, whereas the high positive schizotypy cluster had intact self-esteem and high resilience and its elevated absorption may hold the promise for adaptive outcomes such as creativity and positive spirituality. However, the high positive schizotypy cluster lacked self-concept clarity. The results suggest that individuals showing high positive and low negative schizotypy demonstrate features promoting mental well-being to an extent that is higher than in all the other clusters, while their self-concept impairment is similar to that observed in the high and the disorganised-interpersonal schizotypy clusters. Better understanding of these factors could be informative for prevention and treatment of psychosis-spectrum disorders.
Long-term trends of tuberculosis incidence and mortality in four central African countries
Authors: Martial Nodjimadji Tamlengar , Mubarik Sumaira , Yu Chuanhua
Published: August 2021

Tuberculosis (TB) incidence and mortality rates are still high in Sub-Saharan Africa, and the knowledge about the current patterns is valuable for policymaking to decrease the TB burden. Based on the Global Burden of Disease (GBD) study 2019, we used a Joinpoint regression analysis to examine the variations in the trends of TB incidence and mortality, and the age-period-cohort statistical model to evaluate their risks associated with age, period, and cohort in males and females from Cameroon (CAM), Central African Republic (CAR), Chad, and the Democratic Republic of the Congo (DRC). In the four countries, TB incidence and mortality rates displayed decreasing trends in men and women; except for the males from DRC that recorded an almost steady pattern in the trend of TB incidence between 1990 and 2019. TB incidence and mortality rates decreased according to the overall annual percentage changes over the adjusted age category in men and women of the four countries, and CAM registered the highest decrease. Although TB incidence and mortality rates increased with age between 1990 and 2019, the male gender was mainly associated with the upward behaviors of TB incidence rates, and the female gender association was with the upward behaviors of TB mortality rates. Males and females aged between 15–54 and 15–49 years old were evaluated as the population at high risks of TB incidence and mortality respectively in CAM, CAR, Chad, and DRC. The period and cohort relative risks (RRs) both declined in men and women of the four countries although there were some upward behaviors in their trends. Relatively to the period and cohort RRs, females and males from CAM recorded the most significant decrease compared to the rest of the countries. New public health approaches and policies towards young adults and adults, and a particular focus on elderlies’ health and life conditions should be adopted in CAM, CAR, DRC, and Chad to rapidly decrease TB incidence and mortality in both genders of the four countries.
Wide range and highly linear signal processed systematic humidity sensor array using Methylene Blue and Graphene composite
Authors: Khan Muhammad Umair , Hassan Gul , Shaukat Rayyan Ali , Saqib Qazi Muhammad , Chougale Mahesh Y. , Kim Jungmin , Bae Jinho
Published: August 2021

This paper proposes a signal processed systematic 3 × 3 humidity sensor array with all range and highly linear humidity response based on different particles size composite inks and different interspaces of interdigital electrodes (IDEs). The fabricated sensors are patterned through a commercial inkjet printer and the composite of Methylene Blue and Graphene with three different particle sizes of bulk Graphene Flakes (BGF), Graphene Flakes (GF), and Graphene Quantum Dots (GQD), which are employed as an active layer using spin coating technique on three types of IDEs with different interspaces of 300, 200, and 100 µm. All range linear function (0–100% RH) is achieved by applying the linear combination method of nine sensors in the signal processing field, where weights for linear combination are required, which are estimated by the least square solution. The humidity sensing array shows a fast response time (Tres) of 0.2 s and recovery time (Trec) of 0.4 s. From the results, the proposed humidity sensor array opens a new gateway for a wide range of humidity sensing applications with a linear function.
Regions of intensification of extreme snowfall under future warming
Authors: Quante Lennart , Willner Sven N. , Middelanis Robin , Levermann Anders
Published: August 2021

Due to climate change the frequency and character of precipitation are changing as the hydrological cycle intensifies. With regards to snowfall, global warming has two opposing influences; increasing humidity enables intense snowfall, whereas higher temperatures decrease the likelihood of snowfall. Here we show an intensification of extreme snowfall across large areas of the Northern Hemisphere under future warming. This is robust across an ensemble of global climate models when they are bias-corrected with observational data. While mean daily snowfall decreases, both the 99th and the 99.9th percentiles of daily snowfall increase in many regions in the next decades, especially for Northern America and Asia. Additionally, the average intensity of snowfall events exceeding these percentiles as experienced historically increases in many regions. This is likely to pose a challenge to municipalities in mid to high latitudes. Overall, extreme snowfall events are likely to become an increasingly important impact of climate change in the next decades, even if they will become rarer, but not necessarily less intense, in the second half of the century.
Phase stable swept-source optical coherence tomography with active mode-locking laser for contrast enhancements of retinal angiography
Authors: Park Kwan Seob , Park Eunwoo , Lee Hwidon , Lee Hyun-Ji , Lee Sang-Won , Eom Tae Joong
Published: August 2021

Swept-source optical coherence tomography (SS-OCT) is an attractive high-speed imaging technique for retinal angiography. However, conventional swept lasers vary the cavity length of the laser mechanically to tune the output wavelength. This causes sweep-timing jitter and hence low phase stability in OCT angiography. Here, we improve an earlier phase-stabilized, akinetic, SS-OCT angiography (OCTA) method by introducing coherent averaging. We develop an active mode-locking (AML) laser as a high phase-stable akinetic swept source for the OCTA system. The phase stability of the improved system was analyzed, and the effects of coherent averaging were validated using a retina phantom. The effectiveness of the coherent averaging method was further confirmed by comparing coherently and conventionally averaged en face images of human retinal vasculature for their contrast-to-noise ratio, signal-to-noise ratio, and vasculature connectivity. The contrast-to-noise ratio was approximately 1.3 times larger when applying the coherent averaging method in the human retinal experiment. Our coherent averaging method with the high phase-stability AML laser source for OCTA provides a valuable tool for studying healthy and diseased retinas.
Comparative analysis of biological versus chemical synthesis of palladium nanoparticles for catalysis of chromium (VI) reduction
Authors: Matsena Mpumelelo T. , Chirwa Evans M. N.
Published: August 2021

The discharge of hexavalent chromium [Cr(VI)] from several anthropogenic activities leads to environmental pollution. In this study, we explore a simple yet cost effective method for the synthesis of palladium (Pd) nanoparticles for the treatment of Cr(VI). The presence of elemental Pd [Pd(0)] was confirmed by scanning electron microscope (SEM), electron dispersive spectroscopy and X-ray diffraction (XRD). We show here that the biologically synthesized nanoparticles (Bio-PdNPs) exhibit improved catalytic reduction of Cr(VI) due to their size being smaller and also being highly dispersed as compared to chemically synthesized nanoparticles (Chem-PdNPs). The Langmuir–Hinshelwood mechanism was successfully used to model the kinetics. Using this model, the Bio-PdNPs were shown to perform better than Chem-PdNPs due to the rate constant (kbio = 6.37 mmol s−1 m−2) and Cr(VI) adsorption constant (KCr(VI),bio = 3.11 × 10−2 L mmol−1) of Bio-PdNPs being higher than the rate constant (kchem = 3.83 mmol s−1 m−2) and Cr(VI) adsorption constant (KCr(VI),chem = 1.14 × 10−2 L mmol−1) of Chem-PdNPs. In addition, product inhibition by trivalent chromium [Cr(III)] was high in Chem-PdNPs as indicated by the high adsorption constant of Cr(III) in Chem-PdNPs of KCr(III),chem = 52.9 L mmol−1as compared to the one for Bio-PdNPs of KCr(III),bio = 2.76 L mmol−1.
Extensive phenotypic characterisation of a human TDP-43Q331K transgenic mouse model of amyotrophic lateral sclerosis (ALS)
Authors: Watkins Jodie A. , Alix James J. P. , Shaw Pamela J. , Mead Richard J.
Published: August 2021

The majority of preclinical studies in ALS have relied on transgenic models with overexpression of mutant human superoxide dismutase 1 (SOD1), widely regarded to have failed in terms of translation of therapeutic effects. However, there are still no widely accepted models of other genetic subtypes of ALS. The majority of patients show ubiquitinated cytoplasmic inclusions of TAR DNA binding protein of 43 kilodaltons (TDP-43) in spinal motor neurons at the end stage of disease and a small proportion have mutations in TARDBP, the gene encoding TDP-43. TDP-43 transgenic mouse models have been produced, but have not been widely adopted. Here, we characterised one of these models available from the Jackson Laboratory in detail. Compared to TDP-43WT mice, TDP-43Q331K mice had 43% less hindlimb muscle mass at 6 months and a 73% reduction in hindlimb compound muscle action potential at 8 months of age. Rotarod and gait analysis indicated motor system decline with elevated weight gain. At the molecular level, the lack of TDP-43 cellular pathology was confirmed with a surprising increase in nuclear TDP-43 in motor neurons. Power analysis indicated group sizes of 12–14 mice are needed to detect 10–20% changes in measured parameters with a power of 80%, providing valid readouts for preclinical testing. Overall, this model may represent a useful component of multi-model pre-clinical therapeutic studies for ALS.
Impact of a nutrition consultation on the rate of high output stoma-related readmission: an ambispective cohort study
Authors: Santamaría Manuela Moreno , Villafranca José Javier Arenas , Abilés Jimena , Ruiz Francisco Rivas , Navarro Pilar Utrilla , Goitia Begoña Tortajada
Published: August 2021

The aims of this study were to assess the impact of a follow-up nutrition consultation for ostomy patients on the rate of high output stoma (HOS)-related readmissions, as well as on the detection of poor nutritional status and their management, and to determine the associated economic impact. A single-centre ambispective cohort study was conducted in which all adult patients undergoing intestinal resection and stoma creation were recruited. Two nutrition consultations were established for early follow-up after hospital discharge and patients were prospectively included. Additionally, a retrospective search was carried out to include a control group. In both groups, a 12-month follow-up was conducted to record readmissions associated with high output stoma. A multivariate logistic regression was performed. Statistical significance level was established at p < 0.05. 170 patients were recruited, 85 patients in each cohort. Demographic data and clinical characteristics were recorded. A significant difference was observed in HOS-related readmissions, with readmission rates of 28.6% vs 10.3% in the retrospective and prospective cohort, respectively. At the first follow-up consultation, 50.5% of patients presented some degree of protein-calorie malnutrition. A statistically significant improvement in nutritional status was observed in the second evaluation. The intervention carried out resulted in a total saving of €24,175. Early follow-up of patients after discharge resulted in a significant reduction in the rate of HOS-related readmissions and allowed to identify a high percentage of patients with malnutrition. The cost analysis showed the process to be a cost-effective improvement.
The grades and freshness assessment of eggs based on density detection using machine vision and weighing sensor
Authors: Harnsoongnoen Supakorn , Jaroensuk Nuananong
Published: August 2021

The water displacement and flotation are two of the most accurate and rapid methods for grading and assessing freshness of agricultural products based on density determination. However, these techniques are still not suitable for use in agricultural inspections of products such as eggs that absorb water which can be considered intrusive or destructive and can affect the result of measurements. Here we present a novel proposal for a method of non-destructive, non-invasive, low cost, simple and real—time monitoring of the grading and freshness assessment of eggs based on density detection using machine vision and a weighing sensor. This is the first proposal that divides egg freshness into intervals through density measurements. The machine vision system was developed for the measurement of external physical characteristics (length and breadth) of eggs for evaluating their volume. The weighing system was developed for the measurement of the weight of the egg. Egg weight and volume were used to calculate density for grading and egg freshness assessment. The proposed system could measure the weight, volume and density with an accuracy of 99.88%, 98.26% and 99.02%, respectively. The results showed that the weight and freshness of eggs stored at room temperature decreased with storage time. The relationship between density and percentage of freshness was linear for the all sizes of eggs, the coefficient of determination (R2) of 0.9982, 0.9999, 0.9996, 0.9996 and 0.9994 for classified egg size classified 0, 1, 2, 3 and 4, respectively. This study shows that egg freshness can be determined through density without using water to test for water displacement or egg flotation which has future potential as a measuring system important for the poultry industry.
Role of the motor cortex in the generation of classically conditioned eyelid and vibrissae responses
Authors: López-Ramos Juan C. , Delgado-García José M.
Published: August 2021

The eyelid motor system has been used for years as an experimental model for studying the neuronal mechanisms underlying motor and cognitive learning, mainly with classical conditioning procedures. Nonetheless, it is not known yet which brain structures, or neuronal mechanisms, are responsible for the acquisition, storage, and expression of these motor responses. Here, we studied the temporal correlation between unitary activities of identified eyelid and vibrissae motor cortex neurons and the electromyographic activity of the orbicularis oculi and vibrissae muscles and magnetically recorded eyelid positions during classical conditioning of eyelid and vibrissae responses, using both delay and trace conditioning paradigms in behaving mice. We also studied the involvement of motor cortex neurons in reflexively evoked eyelid responses and the kinematics and oscillatory properties of eyelid movements evoked by motor cortex microstimulation. Results show the involvement of the motor cortex in the performance of conditioned responses elicited during the classical conditioning task. However, a timing correlation analysis showed that both electromyographic activities preceded the firing of motor cortex neurons, which must therefore be related more with the reinforcement and/or proper performance of the conditioned responses than with their acquisition and storage.
Impaired impulse inhibition of emotional stimuli in patients with borderline personality disorder
Authors: Yang Huihui , Liu Qian , Peng Wanrong , Liu Zhaoxia , Chu Jun , Zheng Kaili , Cao Wanyi , Yi Jinyao
Published: August 2021

This study was aimed to investigate whether BPD patients showed impaired impulse inhibition of emotional and non-emotional stimuli and to explore relevant neuroelectrophysiological mechanisms. A total of 32 BPD patients and 32 matched healthy controls were recruited. Self-reported scales were used to measure psychiatric symptoms. The event-related potentials (ERPs) were recorded when subjects were performing neutral and emotional Stop Signal Task (SST). Group differences in self-reported scores, behavioral variables and ERPs were compared. The BPD group scored significantly higher on impulsivity, severity of BPD symptoms, levels of depression and anxiety than the control group. In neutral SST, no significant group differences were detected in the amplitude and latency of ERPs components induced. In emotional SST, the P2 amplitude of negative emotion was significantly larger than that of neutral emotion in Go trials. In Stop trials, the P2 amplitude of BPD group was significantly smaller than that of control group, and the N2 amplitude of BPD group was significantly greater than that of control group. BPD patients showed impaired inhibition of emotional stimuli rather than non-emotional stimuli. The deficits of emotional impulse control mainly exhibit at the early attention, stimulus evaluation and conflict detection stages.
Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand
Authors: Khumpim Pacharaporn , Chawengkirttikul Runglawan , Junsiri Witchuta , Watthanadirek Amaya , Poolsawat Napassorn , Minsakorn Sutthida , Srionrod Nitipon , Anuracpreeda Panat
Published: August 2021

Leucocytozoon sabrazesi is the intracellular protozoa of leucocytozoonosis, which is transmitted by the insect vectors and affects chickens in most subtropical and tropical regions of the globe, except South America, and causing enormous economic losses due to decreasing meat yield and egg production. In this study, L. sabrazesi gametocytes have been observed in the blood smears, and molecular methods have been used to analyse the occurrence and genetic diversity of L. sabrazesi in blood samples from 313 chickens raised in northern, western and southern parts of Thailand. The nested polymerase chain reaction (nested PCR) assay based on the cytb gene revealed that 80.51% (252/313) chickens were positive of L. sabrazesi. The phylogenetic analysis indicated that L. sabrazesi cytb gene is conserved in Thailand, showed 2 clades and 2 subclades with similarity ranged from 89.5 to 100%. The diversity analysis showed 13 and 18 haplotypes of the sequences from Thailand and from other countries, respectively. The entropy analyses of nucleic acid sequences showed 26 high entropy peaks with values ranging from 0.24493 to 1.21056, while those of amino acid sequences exhibited 5 high entropy peaks with values ranging from 0.39267 to 0.97012. The results; therefore, indicate a high molecular occurrence of L. sabrazesi in chicken blood samples with the associated factors that is statistically significant (p < 0.05). Hence, our results could be used to improve the immunodiagnostic methods and to find appropriate preventive control strategies or vaccination programs against leucocytozoonosis in order to mitigate or eliminate the harmful impact of this infection on chicken industry.
Enhanced thermoelectric performance of UV-curable silver (I) selenide-based composite for energy harvesting
Authors: Park Dabin , Lee Seonmin , Kim Jooheon
Published: August 2021

Thermoelectric (TE) composites, with photocured resin as the matrix and Ag2Se (AS) as the filler, are synthesized by a digital-light-processing (DLP) based 3D printer. The mixture of diurethane dimethacrylate (DUDMA) and isobornyl acrylate (IBOA) is used as a UV-curable resin because of their low viscosity and high miscibility. Scanning electron microscopy (FE-SEM) images confirm that the filler retains its shape and remains after the UV-curing process. After completing curing, the mechanical and thermoelectric properties of the composite with different AS contents were measured. The addition of the AS filler increases the thermoelectric properties of the cured resin. When the AS contents increase by 30 wt.%, the maximum power factor was obtained (~ 51.5 μW/m·K2 at room temperature). Additionally, due to the phonon scattering effect between the interfaces, the thermal conductivity of composite is lower than that of pristine photoresin. The maximum thermoelectric figure of merit (ZT) is ~ 0.12, which is achieved with 30 wt.% of AS at 300 K with the enhanced power factor and reduced thermal conductivity. This study presents a novel manufacturing method for a thermoelectric composite using 3D printing.
Integrated bioinformatics analysis of differentially expressed genes and immune cell infiltration characteristics in Esophageal Squamous cell carcinoma
Authors: Feng Zitong , Qu Jingge , Liu Xiao , Liang Jinghui , Li Yongmeng , Jiang Jin , Zhang Huiying , Tian Hui
Published: August 2021

Esophageal squamous cell carcinoma (ESCC) is a life-threatening thoracic tumor with a poor prognosis. The role of molecular alterations and the immune microenvironment in ESCC development has not been fully elucidated. The present study aimed to elucidate key candidate genes and immune cell infiltration characteristics in ESCC by integrated bioinformatics analysis. Nine gene expression datasets from the Gene Expression Omnibus (GEO) database were analysed to identify robust differentially expressed genes (DEGs) using the robust rank aggregation (RRA) algorithm. Functional enrichment analyses showed that the 152 robust DEGs are involved in multiple processes in the tumor microenvironment (TME). Immune cell infiltration analysis based on the 9 normalized GEO microarray datasets was conducted with the CIBERSORT algorithm. The changes in macrophages between ESCC and normal tissues were particularly obvious. In ESCC tissues, M0 and M1 macrophages were increased dramatically, while M2 macrophages were decreased. A robust DEG-based protein–protein interaction (PPI) network was used for hub gene selection with the CytoHubba plugin in Cytoscape. Nine hub genes (CDA, CXCL1, IGFBP3, MMP3, MMP11, PLAU, SERPINE1, SPP1 and VCAN) had high diagnostic efficiency for ESCC according to receiver operating characteristic (ROC) curve analysis. The expression of all hub genes except MMP3 and PLAU was significantly related to macrophage infiltration. Univariate and multivariate regression analyses showed that a 7-gene signature constructed from the robust DEGs was useful for predicting ESCC prognosis. Our results might facilitate the exploration of potential targeted TME therapies and prognostic evaluation in ESCC.
Isoforms of soluble vascular endothelial growth factor in stress-related mental disorders: a cross-sectional study
Authors: Wallensten Johanna , Mobarrez Fariborz , Åsberg Marie , Borg Kristian , Beser Aniella , Wilczek Alexander , Nager Anna
Published: August 2021

Vascular endothelial growth factor (VEGF) has been implicated in the pathophysiology of stress-related mental disorders. However, VEGF levels have seldom been compared across mental disorders and never by isoforms. Pathophysiological processes involving leakage of astrocyte-derived extracellular vesicles (EVs) across the blood–brain barrier could be associated with VEGF levels in patients with stress-related mental disorders. This cross-sectional study compared plasma levels of VEGF121, VEGF165, and VEGF121 + VEGF165 (VEGFtotal) in patients with stress-induced exhaustion disorder (SED) (n = 31), patients with major depressive disorder (MDD) (n = 31), and healthy controls (n = 61). It also analyzed the correlation between VEGF and astrocyte-derived EVs in plasma. An enzyme-linked immunosorbent assay (ELISA) was used to measure VEGF121 and VEGF165 in citrate plasma, and flow cytometry was used to measure astrocyte-derived EVs in plasma. The mean concentration of soluble VEGF121 (sVEGF121) was significantly higher in patients with SED than healthy controls (P = 0.043). Mean sVEGF165 was significantly lower in patients with MDD than patients with SED (P = 0.004) or healthy controls (P = 0.037). Mean sVEGFtotal was significantly higher in patients with SED than in patients with MDD (P = 0.021) and also higher in patients with SED than healthy controls (P = 0.040). Levels of sVEGF121 were positively correlated with levels of astrocyte-derived EVs only in patients with SED (P = 0.0128). The same was true of levels of sVEGFtotal and astrocyte-derived EVs (P = 0.0046). Differing levels of VEGF isoforms may reflect different pathophysiological mechanisms in SED and MDD. Further research is needed to better understand the potential roles of VEGF isoforms and astrocyte-derived EVs in mental disorders.