bluuebunny/crossref_metadata_2025 huggingface dataset:
Polychrome Marbles in Christian Churches: Examples from the Antependium of Baroque Altars in Apulia (Southern Italy)
Authors: Acquafredda Pasquale , Micheletti Francesca , Fioretti Giovanna
Published: June 2024

The antependia, realized in polychrome marbles during the Baroque period in two Christian churches of Apulia (Southern Italy), were on-site petrographically investigated by the naked eye to obtain information regarding the types of used rocks; the precision in the making of the marble tesserae was also assessed. Most of the polychrome marbles recognized were taken from buildings and monuments realized during the Roman imperial period; other marbles quarried during the Baroque period, mainly from Italian geological outcrops, were also identified. The precision in the execution of the tesserae is generally very high and depends not only on the skill of the marble worker but also on the lithotype.
Overexpression of the CpCOR413PM1 Gene from Wintersweet (Chimonanthus praecox) Enhances Cold and Drought Tolerance in Arabidopsis
Authors: Deng Yeyuan , Lin Yi , Wei Guo , Hu Xiaoqian , Zheng Yanghui , Ma Jing
Published: June 2024

Wintersweet (Chimonanthus praecox (L.) Link) is a commercial significance woody plant. As a rare winter-blooming plant, C. praecox is widely distributed and highly adaptable to various climates, especially low temperatures. In this study, we identified the COR413 plasma membrane gene CpCOR413PM1 in wintersweet. CpCOR413PM1 was expressed in all tissues of the plant, with the highest expression observed in the root and floral parts. Cultivation under 4 °C and with treatment of ABA led to the up-regulated expression of CpCOR413PM1. The expression of recombinant CpCOR413PM1 protein in Escherichia coli resulted in the tissues’ increased resilience to cold and drought stress. In vitro enzyme activity assays confirmed the protective impact of the CpCOR413PM1 protein on superoxide dismutase under low-temperature stress. Furthermore, the overexpression of CpCOR413PM1 in Arabidopsis thaliana resulted in increased cold and drought tolerance and ABA inhibited transgenic Arabidopsis seed germination. The CpCOR413PM1 gene promoter can influence expression of the GUS reporter gene under conditions of 4 °C, 42 °C and abscisic acid. Overall, our study demonstrates that CpCOR413PM1 plays a significate role in cold and drought stress. Our findings strengthen the knowledge of the molecular mechanisms underlying wintersweet’s tolerance to stress and lay the groundwork for the future investigation of the functions of the COR gene family.
Comparative and Phylogenetic Analyses of the Complete Chloroplast Genomes of Four Ottelia Species
Authors: Tang Jianmin , Zou Rong , Huang Ke , Gao Limei , Tang Fengluan , Ding Tao , Jiang Yunsheng , Wei Xiao
Published: June 2024

The genus Ottelia comprises approximately 21 submerged macrophyte species primarily found in tropical Africa and Southeast Asia. The classification of several Ottelia species as vulnerable under the criteria “A2c” in the China Species Red List emphasizes the urgency of establishing a credible taxonomy. The ambiguity in taxonomy and evolutionary history persists, primarily stemming from the absence of a robust phylogeny at the species level. The rapid progress in high-throughput sequencing technology has facilitated the retrieval of complete chloroplast (cp) genome sequences, offering a molecular foundation for conducting phylogenetic analyses. In this study, the entire cp genomes of five samples of four Ottelia species were sequenced. All five Ottelia samples exhibited a circular, quadripartite-structured molecule, with lengths ranging from 156,823 to 162,442 bp. A total of 75–88 simple sequence repeats (SSRs) in the cp genomes of the five Ottelia samples were observed, which could be used for species identification. A preliminary phylogenetic analysis revealed that O. fengshanensis, O. acuminata, and O. guanyangensis clustered with strong support (100 BS). O. acuminata var. jingxiensis was resolved as a sister to O. acuminata var. lunanensis (100 BS), and both were further found to be sisters to O. balansae. The widely distributed O. alismoides exhibited a close phylogenetic relationship as the sister taxon to all the Chinese endemic taxa, supported by robust values. Together, our thorough examination of the entire cp genomes of the five Ottelia samples provides eminent insights into reconstructing their phylogeny. Furthermore, it illuminates the evolutionary dynamics of the cp genome within the Ottelia genus.
Nitrogen Fixation and Growth of Potted Olive Plants through Foliar Application of a Nitrogen-Fixing Microorganism
Authors: Rodrigues Manuel Ângelo , Raimundo Soraia , Correia Carlos M. , Arrobas Margarida
Published: June 2024

Given the importance of biological nitrogen (N) fixation in agroecosystems, using inoculants with phyllosphere N fixers effective across various crops would revolutionize agriculture. In this study, the application of an inoculant prepared from Methylobacterium symbioticum was tested on young olive trees. The pot experiment was arranged in a factorial design, with inoculant (Yes and No) and mineral N applied to the soil [0 (N0), 25 (N25), 50 (N50), and 100 (N100) kg ha−1], and four replicates. The inoculant application did not increase plant dry matter yield (DMY), whereas the application of mineral N had a significant and pronounced effect. The inoculant also did not significantly increase N concentration in tissues, unlike the strong increase observed with N applied to the soil. The inoculant significantly increased plant N recovery, a cumulative effect resulting from small increases in DMY and N concentration in tissues. This increase represented 5.2% more N in plants receiving the inoculant compared to untreated ones. However, only treatments receiving mineral N recorded positive values of fixed N, with the highest value observed in the N50 treatment (12.4%), whereas a negative value (−7.7%) was observed in the N0 treatment. Overall, these low values of fixed N question the economic rationale of using this inoculant by farmers and especially render it unsuitable for organic farming systems, where plants tend to have lower N levels in tissues.
Bioponic Cultivation Using Chicken Droppings to Produce Lettuce Plants (Lactuca sativa rz) Uncontaminated by Trace Metals
Authors: Mununga Katebe Félicien , Szekely Iris , Mpundu Mubemba Michel , Burgeon Clément , Jijakli M. Haïssam
Published: June 2024

Anthropogenic activities have denatured aquatic, terrestrial, and aerial environments throughout the world in general, and in Lubumbashi in particular, where market garden soils have become uncultivable for many plants. Thus, bioponics could be an effective means of producing uncontaminated vegetables in soilless cultivation, not only reducing the amount of fertilizer used and limiting contamination of agricultural produce but also achieving higher yields than in open-ground cultivation. The overall objective of this study was to implement a new bioponic technique for producing liquid fertilizer from chicken manure and utilize it in the organic hydroponic cultivation of lettuce (Lactuca sativa var. Lucrecia) installed on floating raft systems. To achieve this, two types of trials were conducted. The first was aimed at determining the quantities of organic matter to be used in the formulation of nutrient solutions. The second trial aimed to determine the optimal nitrogen concentration to be provided for hydroponic plant growth. Mineralization and/or anaerobic digestion of chicken manure were conducted for 7 days in 200 L barrels. For the first trial, nutrient solutions were created from three different concentrations of chicken manure (0.35%, 3.5%, and 7% dry matter—D.M.). These solutions were then used in bioponic rafts where total ammonia nitrogen (TAN) concentrations were fixed at 150 mg/L. For the second trial, D.M. was fixed at 2.5% for each tested modality, but TAN concentrations varied among them (i.e., 60, 90, and 120 mg/L TAN concentration). Modalities with low D.M. concentration (0.35%) and those with low TAN concentration (60 mg/L) resulted in higher yields than bioponic modalities receiving high concentrations of dry matter or TAN, respectively, for trials 1 and 2. Although the reference chemical solutions generate the greatest yields, bioponic systems operating with chicken manure present a good alternative for the cultivation of vegetables in developing countries with heavily contaminated soils. Indeed, bioponics allows for the production of vegetables in large quantities from animal waste, which does not pose health risks for human consumption. Local vegetable species commonly grown in Lubumbashi should be tested under hydroponic conditions.
Identification of Resistance QTLs to Black Leaf Streak Disease (Due to Pseudocercospora fijiensis) in Diploid Bananas (Musa acuminata)
Authors: Carreel Françoise , Martin Guillaume , Ravel Sébastien , Roussel Véronique , Pages Christine , Habas Rémy , Cantagrel Théo , Guiougou Chantal , Delos Jean-Marie , Hervouet Catherine , Mournet Pierre , D’Hont Angélique , Yahiaoui Nabila , Salmon Frédéric
Published: June 2024

Black Leaf Streak Disease (BLSD), caused by the fungus Pseudocercospora fijiensis, is a recent pandemic and the most economically and environmentally important leaf disease of banana. To assist breeding of varieties with durable resistance to the rapidly evolving P. fijiensis, we used a diploid genitor ‘IDN 110’ with partial resistance to BLSD to search for QTLs. We assessed diploid progeny of 73 hybrids between ‘IDN 110’ and the diploid cultivar ‘Khai Nai On’, which is susceptible to BLSD. Hybrids were phenotyped with artificial inoculation under controlled conditions. This method allowed us to focus on resistance in the early stages of the interaction already identified as strongly influencing BLSD epidemiology. Progeny were genotyped by sequencing. As both parents are heterozygous for large reciprocal translocations, the distribution of recombination was assessed and revealed regions with low recombination rates. Fourteen non-overlapping QTLs of resistance to BLSD were identified of which four main QTLs from the ‘IDN110‘ parent, located on chromosomes 06, 07, 08, and 09, were shown to be of interest for marker-assisted selection. Genes that underline those four QTLs are discussed in the light of previous literature.
The Use of Podcasts as Patient Preparation for Hospital Visits—An Interview Study Exploring Patients’ Experiences
Authors: Frølund Jannie Christina , Løkke Anders , Jensen Hanne Irene , Farver-Vestergaard Ingeborg
Published: June 2024

Introduction: Podcasts have emerged as a promising tool in patient preparation for hospital visits. However, the nuanced experiences of patients who engage with this medium remain underexplored. Objectives: This study explored patients’ experiences of receiving information by way of podcasts prior to their hospital visits. Methods: Semi-structured interviews were conducted with patients with suspected chronic obstructive pulmonary disease (COPD), lung cancer, or sleep apnea. The method of data analysis chosen was thematic analysis. Results: Based on data from 24 interviews, five key themes were identified: technical challenges in utilization of podcasts; individual preferences for information prior to hospital visits; building trust and reducing anxiety through podcasts; the role of podcasts as an accessible and convenient source of information; and enhancement of engagement and empowerment through podcasts. Additionally, the study highlighted the critical importance of tailoring podcasts’ content to individual preferences to optimize the delivery of healthcare information. Conclusions: Podcasts can serve as a meaningful supplement to traditional information sources for patients. However, it is important to recognize that not all patients may be able to engage with this medium effectively due to technical challenges or personal preferences.
Characterization of NR1J1 Paralog Responses of Marine Mussels: Insights from Toxins and Natural Activators
Authors: Casas-Rodríguez Antonio , Medrano-Padial Concepción , Jos Angeles , Cameán Ana M. , Campos Alexandre , Fonseca Elza
Published: June 2024

The pregnane X receptor (PXR) is a nuclear hormone receptor that plays a pivotal role in regulating gene expression in response to various ligands, particularly xenobiotics. In this context, the aim of this study was to shed light on the ligand affinity and functions of four NR1J1 paralogs identified in the marine mussel Mytilus galloprovincialis, employing a dual-luciferase reporter assay. To achieve this, the activation patterns of these paralogs in response to various toxins, including freshwater cyanotoxins (Anatoxin-a, Cylindrospermopsin, and Microcystin-LR, -RR, and -YR) and marine algal toxins (Nodularin, Saxitoxin, and Tetrodotoxin), alongside natural compounds (Saint John’s Wort, Ursolic Acid, and 8-Methoxypsoralene) and microalgal extracts (Tetraselmis, Isochrysis, LEGE 95046, and LEGE 91351 extracts), were studied. The investigation revealed nuanced differences in paralog response patterns, highlighting the remarkable sensitivity of MgaNR1J1γ and MgaNR1J1δ paralogs to several toxins. In conclusion, this study sheds light on the intricate mechanisms of xenobiotic metabolism and detoxification, particularly focusing on the role of marine mussel NR1J1 in responding to a diverse array of compounds. Furthermore, comparative analysis with human PXR revealed potential species-specific adaptations in detoxification mechanisms, suggesting evolutionary implications. These findings deepen our understanding of PXR-mediated metabolism mechanisms, offering insights into environmental monitoring and evolutionary biology research.
The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
Authors: Talaverón Rocío , Morado-Díaz Camilo J. , Herrera Alejandro , Gálvez Victoria , Pastor Angel M. , Matarredona Esperanza R.
Published: June 2024

Neural precursor cells (NPCs) that persist in the postnatal/adult subventricular zone (SVZ) express connexins that form hemichannels and gap junctions. Gap junctional communication plays a role in NPC proliferation and differentiation during development, but its relevance on postnatal age remains to be elucidated. In this work we aimed to evaluate the effect of the blockade of gap junctional communication on proliferation and cell fate of NPCs obtained from the SVZ of postnatal rats. NPCs were isolated and expanded in culture as neurospheres. Electron microscopy revealed the existence of gap junctions among neurosphere cells. Treatment of cultures with octanol, a broad-spectrum gap junction blocker, or with Gap27, a specific blocker for gap junctions formed by connexin43, produced a significant decrease in bromodeoxyuridine incorporation. Octanol treatment also exerted a dose-dependent antiproliferative effect on glioblastoma cells. To analyze possible actions on NPC fate, cells were seeded in the absence of mitogens. Treatment with octanol led to an increase in the percentage of astrocytes and oligodendrocyte precursors, whereas the percentage of neurons remained unchanged. Gap27 treatment, in contrast, did not modify the differentiation pattern of SVZ NPCs. Our results indicate that general blockade of gap junctions with octanol induces significant effects on the behavior of postnatal SVZ NPCs, by reducing proliferation and promoting glial differentiation.
Correction: Stepanenko et al. Trypsin Induced Degradation of Amyloid Fibrils. Int. J. Mol. Sci. 2021, 22, 4828
Authors: Stepanenko Olga V. , Sulatsky Maksim I. , Mikhailova Ekaterina V. , Stepanenko Olesya V. , Kuznetsova Irina M. , Turoverov Konstantin K. , Sulatskaya Anna I.
Published: June 2024

In the original [...]
Molecular Pathogenesis of Ischemic and Hemorrhagic Strokes: Background and Therapeutic Approaches
Authors: Maida Carlo Domenico , Norrito Rosario Luca , Rizzica Salvatore , Mazzola Marco , Scarantino Elisa Rita , Tuttolomondo Antonino
Published: June 2024

Stroke represents one of the neurological diseases most responsible for death and permanent disability in the world. Different factors, such as thrombus, emboli and atherosclerosis, take part in the intricate pathophysiology of stroke. Comprehending the molecular processes involved in this mechanism is crucial to developing new, specific and efficient treatments. Some common mechanisms are excitotoxicity and calcium overload, oxidative stress and neuroinflammation. Furthermore, non-coding RNAs (ncRNAs) are critical in pathophysiology and recovery after cerebral ischemia. ncRNAs, particularly microRNAs, and long non-coding RNAs (lncRNAs) are essential for angiogenesis and neuroprotection, and they have been suggested to be therapeutic, diagnostic and prognostic tools in cerebrovascular diseases, including stroke. This review summarizes the intricate molecular mechanisms underlying ischemic and hemorrhagic stroke and delves into the function of miRNAs in the development of brain damage. Furthermore, we will analyze new perspectives on treatment based on molecular mechanisms in addition to traditional stroke therapies.
Molecular Aspects of Inflammation and Lipid Metabolism in Health and Disease: The Role of the Mitochondria
Authors: Sukhorukov Vasily N. , Orekhov Alexander N.
Published: June 2024

Inflammation and lipid metabolism are two deeply interconnected and reciprocally regulated major physiological processes [...]
Molecular Regulation of Thermogenic Mechanisms in Beige Adipocytes
Authors: Yang Siqi , Liu Yingke , Wu Xiaoxu , Zhu Rongru , Sun Yuanlu , Zou Shuoya , Zhang Dongjie , Yang Xiuqin
Published: June 2024

Adipose tissue is conventionally recognized as a metabolic organ responsible for storing energy. However, a proportion of adipose tissue also functions as a thermogenic organ, contributing to the inhibition of weight gain and prevention of metabolic diseases. In recent years, there has been significant progress in the study of thermogenic fats, particularly brown adipose tissue (BAT). Despite this progress, the mechanism underlying thermogenesis in beige adipose tissue remains highly controversial. It is widely acknowledged that beige adipose tissue has three additional thermogenic mechanisms in addition to the conventional UCP1-dependent thermogenesis: Ca2+ cycling thermogenesis, creatine substrate cycling thermogenesis, and triacylglycerol/fatty acid cycling thermogenesis. This paper delves into these three mechanisms and reviews the latest advancements in the molecular regulation of thermogenesis from the molecular genetic perspective. The objective of this review is to provide readers with a foundation of knowledge regarding the beige fats and a foundation for future research into the mechanisms of this process, which may lead to the development of new strategies for maintaining human health.
Gene Expression Analysis of Yeast Strains with a Nonsense Mutation in the eRF3-Coding Gene Highlights Possible Mechanisms of Adaptation
Authors: Maksiutenko Evgeniia M. , Barbitoff Yury A. , Danilov Lavrentii G. , Matveenko Andrew G. , Zemlyanko Olga M. , Efremova Elena P. , Moskalenko Svetlana E. , Zhouravleva Galina A.
Published: June 2024

In yeast Saccharomyces cerevisiae, there are two translation termination factors, eRF1 (Sup45) and eRF3 (Sup35), which are essential for viability. Previous studies have revealed that presence of nonsense mutations in these genes leads to amplification of mutant alleles (sup35-n and sup45-n), which appears to be necessary for the viability of such cells. However, the mechanism of this phenomenon remained unclear. In this study, we used RNA-Seq and proteome analysis to reveal the complete set of gene expression changes that occur during cellular adaptation to the introduction of the sup35-218 nonsense allele. Our analysis demonstrated significant changes in the transcription of genes that control the cell cycle: decreases in the expression of genes of the anaphase promoting complex APC/C (APC9, CDC23) and their activator CDC20, and increases in the expression of the transcription factor FKH1, the main cell cycle kinase CDC28, and cyclins that induce DNA biosynthesis. We propose a model according to which yeast adaptation to nonsense mutations in the translation termination factor genes occurs as a result of a delayed cell cycle progression beyond the G2-M stage, which leads to an extension of the S and G2 phases and an increase in the number of copies of the mutant sup35-n allele.
Closing Editorial: Immunophenotyping in Autoimmune Diseases and Cancer 3.0
Authors: Szebeni Gábor J. , Balog Attila
Published: June 2024

The mammalian immune system is a Janus-faced network of well-coordinated highly specialized cells and biomolecules [...]
Enhancing Erucic Acid and Wax Ester Production in Brassica carinata through Metabolic Engineering for Industrial Applications
Authors: Tesfaye Misteru , Wang Eu Sheng , Feyissa Tileye , Herrfurth Cornelia , Haileselassie Teklehaimanot , Kanagarajan Selvaraju , Feussner Ivo , Zhu Li-Hua
Published: June 2024

Metabolic engineering enables oilseed crops to be more competitive by having more attractive properties for oleochemical industrial applications. The aim of this study was to increase the erucic acid level and to produce wax ester (WE) in seed oil by genetic transformation to enhance the industrial applications of B. carinata. Six transgenic lines for high erucic acid and fifteen transgenic lines for wax esters were obtained. The integration of the target genes for high erucic acid (BnFAE1 and LdPLAAT) and for WEs (ScWS and ScFAR) in the genome of B. carinata cv. ‘Derash’ was confirmed by PCR analysis. The qRT-PCR results showed overexpression of BnFAE1 and LdPLAAT and downregulation of RNAi-BcFAD2 in the seeds of the transgenic lines. The fatty acid profile and WE content and profile in the seed oil of the transgenic lines and wild type grown in biotron were analyzed using gas chromatography and nanoelectrospray coupled with tandem mass spectrometry. A significant increase in erucic acid was observed in some transgenic lines ranging from 19% to 29% in relation to the wild type, with a level of erucic acid reaching up to 52.7%. Likewise, the transgenic lines harboring ScFAR and ScWS genes produced up to 25% WE content, and the most abundant WE species were 22:1/20:1 and 22:1/22:1. This study demonstrated that metabolic engineering is an effective biotechnological approach for developing B. carinata into an industrial crop.
The Impact of the Combined Effect of Inhalation Anesthetics and Iron Dextran on Rats’ Systemic Toxicity
Authors: Odeh Dyana , Oršolić Nada , Adrović Emanuela , Bilandžić Nina , Sedak Marija , Žarković Irena , Lesar Nikola , Balta Vedran
Published: June 2024

Disruption of any stage of iron homeostasis, including uptake, utilization, efflux, and storage, can cause progressive damage to peripheral organs. The health hazards associated with occupational exposure to inhalation anesthetics (IA) in combination with chronic iron overload are not well documented. This study aimed to investigate changes in the concentration of essential metals in the peripheral organs of rats after iron overload in combination with IA. The aim was also to determine how iron overload in combination with IA affects tissue metal homeostasis, hepcidin–ferritin levels, and MMP levels according to physiological, functional, and tissue features. According to the obtained results, iron accumulation was most pronounced in the liver (19×), spleen (6.7×), lungs (3.1×), and kidneys (2.5×) compared to control. Iron accumulation is associated with elevated heavy metal levels and impaired essential metal concentrations due to oxidative stress (OS). Notably, the use of IA increases the iron overload toxicity, especially after Isoflurane exposure. The results show that the regulation of iron homeostasis is based on the interaction of hepcidin, ferritin, and other proteins regulated by inflammation, OS, free iron levels, erythropoiesis, and hypoxia. Long-term exposure to IA and iron leads to the development of numerous adaptation mechanisms in response to toxicity, OS, and inflammation. These adaptive mechanisms of iron regulation lead to the inhibition of MMP activity and reduction of oxidative stress, protecting the organism from possible damage.
Prognostic and Predictive Potential of CCL5 Expression in Muscle-Invasive Bladder Cancer Patients
Authors: Smolka Cedric , Eckstein Markus , Jung Rudolf , Lieb Verena , Sikic Danijel , Stöhr Robert , Bahlinger Veronika , Bertz Simone , Kehlen Astrid , Hartmann Arndt , Wullich Bernd , Taubert Helge , Wach Sven
Published: June 2024

Bladder cancer (BC) is the 12th most commonly diagnosed cancer worldwide. Although there are several well-established molecular and immunological classifications, prognostic and predictive markers for tumor cells and immune cells are still needed. Using a tissue microarray, we analyzed the expression of the chemokine CC motif ligand 5 (CCL5) by immunohistochemistry (IHC) in 175 muscle-invasive BC samples. The application of a single cutoff for the staining status of tumor cells (TCs; positive vs. negative) and immune cells (ICs; positive vs. negative) revealed 75 patients (42.9%) and 123 patients (70.3%) with CCL5-positive TCs or ICs, respectively. IHC results were associated with prognostic and predictive data. Multivariate Cox regression analysis revealed that positive CCL5 staining in TCs was associated with significantly shorter disease-specific survival (DSS; RR = 1.51; p = 0.047), but CCL5-negative ICs were associated with significantly shorter overall survival (OS; RR = 1.66; p = 0.005), DSS (RR = 2.02; p = 0.001) and recurrence-free survival (RFS; RR = 1.94; p = 0.002). Adjuvant chemotherapy was favorable for patients with CCL5-negative ICs for OS (RR = 0.30; p = 0.006), DSS (RR = 0.36; p = 0.022) and RFS (RR = 0.41; p = 0.046) but not for patients with CCL5-positive ICs, except in the subgroup of N1 + N2 patients, where it was associated with better OS. We suggest that CCL5 expression can be a prognostic and predictive marker for muscle-invasive bladder cancer patients.
Fibrostenosing Crohn’s Disease: Pathogenetic Mechanisms and New Therapeutic Horizons
Authors: Mignini Irene , Blasi Valentina , Termite Fabrizio , Esposto Giorgio , Borriello Raffaele , Laterza Lucrezia , Scaldaferri Franco , Ainora Maria Elena , Gasbarrini Antonio , Zocco Maria Assunta
Published: June 2024

Bowel strictures are well recognized as one of the most severe complications in Crohn’s disease, with variable impacts on the prognosis and often needing surgical or endoscopic treatment. Distinguishing inflammatory strictures from fibrotic ones is of primary importance due to the different therapeutic approaches required. Indeed, to better understand the pathogenesis of fibrosis, it is crucial to investigate molecular processes involving genetic factors, cytokines, alteration of the intestinal barrier, and epithelial and endothelial damage, leading to an increase in extracellular matrix synthesis, which ultimately ends in fibrosis. In such a complex mechanism, the gut microbiota also seems to play a role. A better comprehension of molecular processes underlying bowel fibrosis, in addition to radiological and histopathological findings, has led to the identification of high-risk patients for personalized follow-up and testing of new therapies, primarily in preclinical models, targeting specific pathways involving Transforming Growth Factor-β, interleukins, extracellular matrix balance, and gut microbiota. Our review aims to summarize current evidence about molecular factors involved in intestinal fibrosis’ pathogenesis, paving the way for potential diagnostic biomarkers or anti-fibrotic treatments for stricturing Crohn’s disease.
Superelectrophilic Activation of Phosphacoumarins towards Weak Nucleophiles via Brønsted Acid Assisted Brønsted Acid Catalysis
Authors: Zalaltdinova Alena V. , Sadykova Yulia M. , Gazizov Almir S. , Smailov Atabek K. , Syakaev Victor V. , Gerasimova Daria P. , Chugunova Elena A. , Akylbekov Nurgali I. , Zhapparbergenov Rakhmetulla U. , Appazov Nurbol O. , Burilov Alexander R. , Pudovik Michail A. , Alabugin Igor V. , Sinyashin Oleg G.
Published: June 2024

The electrophilic activation of various substrates via double or even triple protonation in superacidic media enables reactions with extremely weak nucleophiles. Despite the significant progress in this area, the utility of organophosphorus compounds as superelectrophiles still remains limited. Additionally, the most common superacids require a special care due to their high toxicity, exceptional corrosiveness and moisture sensitivity. Herein, we report the first successful application of the “Brønsted acid assisted Brønsted acid” concept for the superelectrophilic activation of 2-hydroxybenzo[e][1,2]oxaphosphinine 2-oxides (phosphacoumarins). The pivotal role is attributed to the tendency of the phosphoryl moiety to form hydrogen-bonded complexes, which enables the formation of dicationic species and increases the electrophilicity of the phosphacoumarin. This unmasks the reactivity of phosphacoumarins towards non-activated aromatics, while requiring only relatively non-benign trifluoroacetic acid as the reaction medium.
The Biomedical Applications of Biomolecule Integrated Biosensors for Cell Monitoring
Authors: Song Kyeongseok , Hwang Soon-Jin , Jeon Yangwon , Yoon Youngdae
Published: June 2024

Cell monitoring is essential for understanding the physiological conditions and cell abnormalities induced by various stimuli, such as stress factors, microbial invasion, and diseases. Currently, various techniques for detecting cell abnormalities and metabolites originating from specific cells are employed to obtain information on cells in terms of human health. Although the states of cells have traditionally been accessed using instrument-based analysis, this has been replaced by various sensor systems equipped with new materials and technologies. Various sensor systems have been developed for monitoring cells by recognizing biological markers such as proteins on cell surfaces, components on plasma membranes, secreted metabolites, and DNA sequences. Sensor systems are classified into subclasses, such as chemical sensors and biosensors, based on the components used to recognize the targets. In this review, we aim to outline the fundamental principles of sensor systems used for monitoring cells, encompassing both biosensors and chemical sensors. Specifically, we focus on biosensing systems in terms of the types of sensing and signal-transducing elements and introduce recent advancements and applications of biosensors. Finally, we address the present challenges in biosensor systems and the prospects that should be considered to enhance biosensor performance. Although this review covers the application of biosensors for monitoring cells, we believe that it can provide valuable insights for researchers and general readers interested in the advancements of biosensing and its further applications in biomedical fields.
Bidirectional Two-Sample Mendelian Randomization Study of Immunoglobulin G N-Glycosylation and Senescence-Associated Secretory Phenotype
Authors: Wang Haotian , Liu Di , Meng Xiaoni , Sun Wenxin , Li Cancan , Lu Huimin , Zheng Deqiang , Wu Lijuan , Sun Shengzhi , Wang Youxin
Published: June 2024

Observational studies revealed changes in Immunoglobulin G (IgG) N-glycosylation during the aging process. However, it lacks causal insights and remains unclear in which direction causal relationships exist. The two-sample bidirectional Mendelian randomization (MR) design was adopted to explore causal associations between IgG N-glycans and the senescence-associated secretory phenotype (SASP). Inverse variance weighted (IVW) and Wald ratio methods were used as the main analyses, supplemented by sensitivity analyses. Forward MR analyses revealed causal associations between the glycan peak (GP) and SASP, including GP6 (odds ratio [OR] = 0.428, 95% confidence interval [CI] = 0.189–0.969) and GP17 (OR = 0.709, 95%CI = 0.504–0.995) with growth/differentiation factor 15 (GDF15), GP19 with an advanced glycosylation end-product-specific receptor (RAGE) (OR = 2.142, 95%  CI  = 1.384–3.316), and GP15 with matrix metalloproteinase 2 (MMP2) (OR = 1.136, 95%  CI =1.008–1.282). The reverse MR indicated that genetic liability to RAGE was associated with increased levels of GP17 (OR = 1.125, 95%  CI  = 1.003–1.261) and GP24 (OR = 1.222, 95%  CI  = 1.046–1.428), while pulmonary and activation-regulated chemokines (PARC) exhibited causal associations with GP10 (OR = 1.269, 95%  CI  = 1.048–1.537) and GP15 (OR = 1.297, 95%  CI = 1.072–1.570). The findings provided suggested evidence on the bidirectional causality between IgG N-glycans and SASP, which might reveal potential regulatory mechanisms.
Detection and Characterisation of SARS-CoV-2 in Eastern Province of Zambia: A Retrospective Genomic Surveillance Study
Authors: Shempela Doreen Mainza , Chambaro Herman M. , Sikalima Jay , Cham Fatim , Njuguna Michael , Morrison Linden , Mudenda Steward , Chanda Duncan , Kasanga Maisa , Daka Victor , Kwenda Geoffrey , Musonda Kunda , Munsaka Sody , Chilengi Roma , Sichinga Karen , Simulundu Edgar
Published: June 2024

Mutations have driven the evolution and development of new variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with potential implications for increased transmissibility, disease severity and vaccine escape among others. Genome sequencing is a technique that allows scientists to read the genetic code of an organism and has become a powerful tool for studying emerging infectious diseases. Here, we conducted a cross-sectional study in selected districts of the Eastern Province of Zambia, from November 2021 to February 2022. We analyzed SARS-CoV-2 samples (n = 76) using high-throughput sequencing. A total of 4097 mutations were identified in 69 SARS-CoV-2 genomes with 47% (1925/4097) of the mutations occurring in the spike protein. We identified 83 unique amino acid mutations in the spike protein of the seven Omicron sublineages (BA.1, BA.1.1, BA.1.14, BA.1.18, BA.1.21, BA.2, BA.2.23 and XT). Of these, 43.4% (36/83) were present in the receptor binding domain, while 14.5% (12/83) were in the receptor binding motif. While we identified a potential recombinant XT strain, the highly transmissible BA.2 sublineage was more predominant (40.8%). We observed the substitution of other variants with the Omicron strain in the Eastern Province. This work shows the importance of pandemic preparedness and the need to monitor disease in the general population.
Light-Field Image Compression Based on a Two-Dimensional Prediction Coding Structure
Authors: Shao Jianrui , Bai Enjian , Jiang Xueqin , Wu Yun
Published: June 2024

Light-field images (LFIs) are gaining increased attention within the field of 3D imaging, virtual reality, and digital refocusing, owing to their wealth of spatial and angular information. The escalating volume of LFI data poses challenges in terms of storage and transmission. To address this problem, this paper introduces an MSHPE (most-similar hierarchical prediction encoding) structure based on light-field multi-view images. By systematically exploring the similarities among sub-views, our structure obtains residual views through the subtraction of the encoded view from its corresponding reference view. Regarding the encoding process, this paper implements a new encoding scheme to process all residual views, achieving lossless compression. High-efficiency video coding (HEVC) is applied to encode select residual views, thereby achieving lossy compression. Furthermore, the introduced structure is conceptualized as a layered coding scheme, enabling progressive transmission and showing good random access performance. Experimental results demonstrate the superior compression performance attained by encoding residual views according to the proposed structure, outperforming alternative structures. Notably, when HEVC is employed for encoding residual views, significant bit savings are observed compared to the direct encoding of original views. The final restored view presents better detail quality, reinforcing the effectiveness of this approach.
Analysis of the Epidemic Curve of the Waves of COVID-19 Using Integration of Functions and Neural Networks in Peru
Authors: Vilca Huayta Oliver , Jimenez Chura Adolfo , Sosa Maydana Carlos , Martínez García Alioska
Published: June 2024

The coronavirus (COVID-19) pandemic continues to claim victims. According to the World Health Organization, in the 28 days leading up to 25 February 2024 alone, the number of deaths from COVID-19 was 7141. In this work, we aimed to model the waves of COVID-19 through artificial neural networks (ANNs) and the sigmoidal–Boltzmann model. The study variable was the global cumulative number of deaths according to days, based on the Peru dataset. Additionally, the variables were adapted to determine the correlation between social isolation measures and death rates, which constitutes a novel contribution. A quantitative methodology was used that implemented a non-experimental, longitudinal, and correlational design. The study was retrospective. The results show that the sigmoidal and ANN models were reasonably representative and could help to predict the spread of COVID-19 over the course of multiple waves. Furthermore, the results were precise, with a Pearson correlation coefficient greater than 0.999. The computational sigmoidal–Boltzmann model was also time-efficient. Moreover, the Spearman correlation between social isolation measures and death rates was 0.77, which is acceptable considering that the social isolation variable is qualitative. Finally, we concluded that social isolation measures had a significant effect on reducing deaths from COVID-19.
A Taxonomic Revision of the Genus Cleopus Dejean, 1821 (Coleoptera, Curculionidae), with Descriptions of 13 New Species
Authors: Košťál Michael , Caldara Roberto
Published: June 2024

The genus Cleopus Dejean, 1821 is herein revised for the first time. Based on adult morphological characteristics, 18 species are recognized as valid. Thirteen species, all distributed in the Eastern Palaearctis or Oriental region, are described as new: C. aduncirostris sp. n.; C. cognatus sp. n.; C. confusus sp. n.; C. dohertyi sp. n.; C. hajeki sp. n.; C. lirenae sp. n.; C. longitarsis sp. n.; C. minutus sp. n.; C. pallidisquamosus sp. n.; C. parvidentatus sp. n.; C. philippinensis sp. n.; C. simillimus sp. n.; and C. subaequalis sp. n. Lectotypes of following two valid species and three synonyms were designated: Curculio solani Fabricius, 1792; Curculio pulchellus Herbst, 1795; Cionus setiger Germar, 1821; Curculio immunis Marsham, 1802; and Cleopus pulchellus rigidus Stephens, 1831. Neotypes of Curculio perpensus Rossi, 1792 and Cleopus pulchellus flavus Stephens, 1832 were designated. The following new synonyms of Cleopus pulchellus (Herbst, 1795) were established: Cleopus pulchellus var. flavus Stephens, 1831 syn. n. and C. pulchellus var. rigidus Stephens, 1831 syn. n.
A Modified Switching Procedure from Temporary to Tunneled Central Venous Dialysis Catheters
Authors: Eberhard Johannes , Bedau Constantin , Chapple Andrew Genius , Klein Julia , Reissfelder Christoph , Kaelsch Anna-Isabelle , Gerken Andreas Lutz Heinrich , Zach Sebastian , Schwenke Kay
Published: June 2024

Background: Tunneled central venous catheters are commonly used for dialysis in patients without a functional permanent vascular access. In an emergent setting, a non-tunneled, temporary central venous catheter is often placed for immediate dialysis. The most critical step in the catheter insertion is venipuncture, which is often a major cause for longer intervention times and procedure-related adverse events. To avoid this critical step when placing a more permanent tunneled catheter, an exchange over a previously placed temporary one can be considered. In this paper, we present a modified switching approach with a separate access site. Methods: In this retrospective analysis of a prospective database, we examined whether this modified technique is non-inferior to a de novo application. Therefore, we included all 396 patients who received their first tunneled dialysis catheter at our site from March 2018 to March 2023. Out of these, 143 patients received the modified approach and 253 the standard de novo ultrasound-guided puncture and insertion. Then, the outcomes of the two groups, including adverse events and infections, were compared by nonparametric tests and multivariable logistic regression. Results: In both groups, the implantations were 100% successful. Catheter explantation due to infection according to CDC criteria was necessary in 18 cases, with no difference between the groups (5.0% vs. 4.4% p = 0.80). The infection rate per 100 days was 0.113 vs. 0.106 in the control group, with a comparable spectrum of bacteria. A total of 12 catheters (3 vs. 9) had to be removed due to a periinterventional complication. An early-onset infection was the reason in two cases (1.3%) in the study group and five in the control group (1.9%). A total misplacement of the catheter occurred in two cases only in the control group. After adjustment for potential confounders via multivariable logistic regression there was not a significant difference in the complication rate (adjusted odds ratio, aOR = 0.53, 95% CI = 0.14–2.03, p = 0.351) but an estimated decreased risk overall based on the average treatment effect of −1.7% in favor of the study group. Conclusions: The present study shows that a catheter exchange leads to no more infections than a de novo placement; hence, it is a feasible method. Moreover, misplacements and control chest X-rays to exclude pneumothorax after venipuncture were completely avoided by exchanging. This approach yields a much lower infection rate than previous reports: 1.3% compared to 2.7% in all existing aggregated studies. The presented approach seems to be superior to existing switching methods. Overall, an exchange can also help to preserve veins for future access, since the same jugular vein is used.
A Case Report Demonstrating Preservation of Vestibular Receptor Function after Transcochlear Removal of an Intracochlear Schwannoma with Extension to the Fundus of the Internal Auditory Canal
Authors: Plontke Stefan K. , Iannacone Francesco P. , Siebolts Udo , Ludwig-Kraus Beatrice , Kösling Sabrina , Wagner Luise
Published: June 2024

Preservation of function is an important goal during surgical management of cochleovestibular schwannomas. We here demonstrate the relief of vertigo and the preservation of function of all five vestibular receptors after removal of an intracochlear schwannoma with extension to the fundus of the internal auditory canal. A 61-year-old male with a five-year history of left-sided deafness, tinnitus, vertigo attacks, and an MRI consistent with an intracochlear schwannoma with limited extension through the modiolus to the fundus of the internal auditory canal (IAC) underwent transcanal, transcochlear total tumor removal and—due to a cerebrospinal fluid leak from the fundus of the IAC—revision surgery with lateral petrosectomy and blind sac closure of the external auditory canal. Despite complete removal of the cochlear partition of the inner ear (total cochlectomy), the patient’s vestibular receptors remained functional, and the vertigo symptoms disappeared. These results show that vestibular labyrinthine function may not only be preserved after partial or subtotal cochlectomy but also after complete cochlear removal. This further confirms the vestibular labyrinth’s robustness and encourages surgical management of transmodiolar schwannomas with limited extension to the fundus of the IAC.
Ten-Year Outcomes in Patients Undergoing Simultaneous Coronary and Renal Angiography—Does Renal Artery Stenosis Matter?
Authors: Kern Adam , Stompór Tomasz , Bojko Krystian , Sienkiewicz Ewa , Pawlak Sebastian , Pawlak Dariusz , Poskrobko Grzegorz , Andrasz Ewa , Gromadziński Leszek , Jalali Rakesh , Onichimowski Dariusz , Piwko Grażyna , Zalewski Artur , Bil Jacek
Published: June 2024

Background: We aimed to characterize the population of consecutive patients undergoing coronary angiography with simultaneous renal artery angiography and assess prognostic factors at a 10 year follow-up. Methods: The KORONEF study was a prospective, single-center, observational, and descriptive study with 492 patients included. We analyzed several baseline demographics, clinical and periprocedural characteristics, and laboratory data, and we assessed the results of coronary angiography and renal artery angiography. Results: The study population consisted of 37.2% women, and the mean age was 64.4 ± 9.9 years (min. 30 years, max. 89 years). Angiography revealed significant renal artery stenosis (RAS) in 35 (7.1%) patients. Among patients with significant RAS (≥50%), we observed more women (57.1% vs. 35.7%, p = 0.011), and patients were older (69.1 ± 10.4 years vs. 64.0 ± 9.7 years, p = 0.005). In the whole population, all-cause death was reported in 29.9% of patients, myocardial infarction (MI) rate—in 11.8%, and stroke—in 4.9%. In the multivariable analysis, independent predictors of death were age 65–75 years (HR 2.88), age > 75 years (HR 8.07), diabetes (HR 1.59), previous MI (HR 1.64), chronic kidney disease (HR 2.22), unstable angina (HR 0.37), and left ventricular ejection fraction > 60% (HR 0.43). Conclusions: Over a 10 year follow-up, the all-cause death rate was 29.9%, showing no statistically significant differences between patients with and without significant RAS.
Association between Opioid–Benzodiazepine Trajectories and Injurious Fall Risk among US Medicare Beneficiaries
Authors: Wang Grace Hsin-Min , Hincapie-Castillo Juan M. , Gellad Walid F. , Jones Bobby L. , Shorr Ronald I. , Yang Seonkyeong , Wilson Debbie L. , Lee Jeannie K. , Reisfield Gary M. , Kwoh Chian K. , Delcher Chris , Nguyen Khoa A. , Harle Christopher A. , Marcum Zachary A. , Lo-Ciganic Wei-Hsuan
Published: June 2024

Background/Objectives: Concurrent opioid (OPI) and benzodiazepine (BZD) use may exacerbate injurious fall risk (e.g., falls and fractures) compared to no use or use alone. Yet, patients may need concurrent OPI-BZD use for co-occurring conditions (e.g., pain and anxiety). Therefore, we examined the association between longitudinal OPI-BZD dosing patterns and subsequent injurious fall risk. Methods: We conducted a retrospective cohort study including non-cancer fee-for-service Medicare beneficiaries initiating OPI and/or BZD in 2016–2018. We identified OPI-BZD use patterns during the 3 months following OPI and/or BZD initiation (i.e., trajectory period) using group-based multi-trajectory models. We estimated the time to first injurious falls within the 3-month post-trajectory period using inverse-probability-of-treatment-weighted Cox proportional hazards models. Results: Among 622,588 beneficiaries (age ≥ 65 = 84.6%, female = 58.1%, White = 82.7%; having injurious falls = 0.45%), we identified 13 distinct OPI-BZD trajectories: Group (A): Very-low OPI-only (early discontinuation) (44.9% of the cohort); (B): Low OPI-only (rapid decline) (15.1%); (C): Very-low OPI-only (late discontinuation) (7.7%); (D): Low OPI-only (gradual decline) (4.0%); (E): Moderate OPI-only (rapid decline) (2.3%); (F): Very-low BZD-only (late discontinuation) (11.5%); (G): Low BZD-only (rapid decline) (4.5%); (H): Low BZD-only (stable) (3.1%); (I): Moderate BZD-only (gradual decline) (2.1%); (J): Very-low OPI (rapid decline)/Very-low BZD (late discontinuation) (2.9%); (K): Very-low OPI (rapid decline)/Very-low BZD (increasing) (0.9%); (L): Very-low OPI (stable)/Low BZD (stable) (0.6%); and (M): Low OPI (gradual decline)/Low BZD (gradual decline) (0.6%). Compared with Group (A), six trajectories had an increased 3-month injurious falls risk: (C): HR = 1.78, 95% CI = 1.58–2.01; (D): HR = 2.24, 95% CI = 1.93–2.59; (E): HR = 2.60, 95% CI = 2.18–3.09; (H): HR = 2.02, 95% CI = 1.70–2.40; (L): HR = 2.73, 95% CI = 1.98–3.76; and (M): HR = 1.96, 95% CI = 1.32–2.91. Conclusions: Our findings suggest that 3-month injurious fall risk varied across OPI-BZD trajectories, highlighting the importance of considering both dose and duration when assessing injurious fall risk of OPI-BZD use among older adults.