bluuebunny/crossref_metadata_2025 huggingface dataset:
NAFLD in Polycystic Ovary Syndrome: Association with PNPLA3 and Metabolic Features
Authors: Recuero Amanda Medeiros , Gomes Larissa Garcia , Maciel Gustavo Arantes Rosa , de Mello Malta Fernanda , Salles Ana Paula Moreira , Vezozzo Denise Cerqueira Paranaguá , Baracat Edmund Chada , Pinho João Renato Rebello , Carrilho Flair José , Stefano José Tadeu , Oliveira Claudia P.
Published: October 2022

Background: The aim of this study was to determine the frequency of the rs738409 polymorphism in the patatin-like phospholipase domain containing 3 (PNPLA3) gene in patients with polycystic ovary syndrome (PCOS) and its impact on nonalcoholic fatty liver disease (NAFLD) risk and severity. We also evaluated other risk factors associated with NAFLD and advanced fibrosis. Methods: This was a cross-sectional study involving 163 patients with PCOS at a tertiary center. Genotyping for the PNPLA3 polymorphism was undertaken using a TaqMan assay. The degree of fibrosis was defined by transient elastography. Results: The prevalence of NAFLD was 72.4%, and the polymorphism was heterozygous in 41.7% and homozygous in 8% of patients. Homeostasis model assessment of insulin resistance ≥ 2.5 was the main factor associated with the risk of developing NAFLD (OR = 4.313, p = 0.022), and its effect was amplified by the polymorphism (OR = 12.198, p = 0.017). Age > 32 years also conferred a higher risk for NAFLD. HDL values ≥ 50 mg/dL conferred protection against the outcome. Metabolic syndrome (OR = 13.030, p = 0.020) and AST > 32 U/L (OR = 9.039, p = 0.009) were independent risk factors for advanced fibrosis. Conclusions: In women with PCOS, metabolic characteristics are more relevant than PNPLA3 polymorphism regarding the risk for NAFLD and its advanced forms, but these factors can act synergistically, increasing disease risk.
Acute Hippocampal Damage as a Prognostic Biomarker for Cognitive Decline but Not for Epileptogenesis after Experimental Traumatic Brain Injury
Authors: Manninen Eppu , Chary Karthik , De Feo Riccardo , Hämäläinen Elina , Andrade Pedro , Paananen Tomi , Sierra Alejandra , Tohka Jussi , Gröhn Olli , Pitkänen Asla
Published: October 2022

It is necessary to develop reliable biomarkers for epileptogenesis and cognitive impairment after traumatic brain injury when searching for novel antiepileptogenic and cognition-enhancing treatments. We hypothesized that a multiparametric magnetic resonance imaging (MRI) analysis along the septotemporal hippocampal axis could predict the development of post-traumatic epilepsy and cognitive impairment. We performed quantitative T2 and T2* MRIs at 2, 7 and 21 days, and diffusion tensor imaging at 7 and 21 days after lateral fluid-percussion injury in male rats. Morris water maze tests conducted between 35–39 days post-injury were used to diagnose cognitive impairment. One-month-long continuous video-electroencephalography monitoring during the 6th post-injury month was used to diagnose epilepsy. Single-parameter and regularized multiple linear regression models were able to differentiate between sham-operated and brain-injured rats. In the ipsilateral hippocampus, differentiation between the groups was achieved at most septotemporal locations (cross-validated area under the receiver operating characteristic curve (AUC) 1.0, 95% confidence interval 1.0–1.0). In the contralateral hippocampus, the highest differentiation was evident in the septal pole (AUC 0.92, 95% confidence interval 0.82–0.97). Logistic regression analysis of parameters imaged at 3.4 mm from the contralateral hippocampus’s temporal end differentiated between the cognitively impaired rats and normal rats (AUC 0.72, 95% confidence interval 0.55–0.84). Neither single nor multiparametric approaches could identify the rats that would develop post-traumatic epilepsy. Multiparametric MRI analysis of the hippocampus can be used to identify cognitive impairment after an experimental traumatic brain injury. This information can be used to select subjects for preclinical trials of cognition-improving interventions.
Role of Translationally Controlled Tumor Protein (TCTP) in the Development of Hypertension and Related Diseases in Mouse Models
Authors: Maeng Jeehye , Lee Kyunglim
Published: October 2022

Translationally controlled tumor protein (TCTP) is a multifunctional protein that plays a wide variety of physiological and pathological roles, including as a cytoplasmic repressor of Na,K-ATPase, an enzyme pivotal in maintaining Na+ and K+ ion gradients across the plasma membrane, by binding to and inhibiting Na,K-ATPase. Studies with transgenic mice overexpressing TCTP (TCTP-TG) revealed the pathophysiological significance of TCTP in the development of systemic arterial hypertension. Overexpression of TCTP and inhibition of Na,K-ATPase result in the elevation of cytoplasmic Ca2+ levels, which increases the vascular contractility in the mice, leading to hypertension. Furthermore, studies using an animal model constructed by multiple mating of TCTP-TG with apolipoprotein E knockout mice (ApoE KO) indicated that TCTP-induced hypertension facilitates the severity of atherosclerotic lesions in vivo. This review attempts to discuss the mechanisms underlying TCTP-induced hypertension and related diseases gleaned from studies using genetically altered animal models and the potential of TCTP as a target in the therapy of hypertension-related pathological conditions.
Long-Term Memory Function Impairments following Sucrose Exposure in Juvenile versus Adult Rats
Authors: Coirini Héctor , Rey Mariana , Gonzalez Deniselle María Claudia , Kruse María Sol
Published: October 2022

We previously described that excessive consumption of sucrose during youth produces fear memory and anxiety-like behavior in adulthood. Here, we evaluated whether high cognitive function is also affected by studying early sucrose consumption in object recognition memory (NOR). Male Sprague Dawley rats were tested for short-term, long-term, and consolidated NOR after 25 days of unlimited sucrose access in juvenile (PD 25–50) or adult age (PD 75–100). All rats spent equal time exploring the two objects during the sample phase T1. When animals were exposed for 2, 24 h or 7 days later to a copy of the objects presented in T1 and a novel object, the sucrose-exposed juvenile group failed to distinguish between the familiar and the novel objects in contrast with the rest of the groups. Sucrose-exposed animals developed hypertriglyceridemia and glucose intolerance, but juvenile animals showed increased fasting glycemia and sustained the glucose intolerance longer. Moreover, sucrose decreased hippocampal proBDNF expression in juveniles while it was increased in adults, and sucrose also increased RAGE expression in adults. The NOR exploration ratio correlated negatively with basal glycemia and positively with proBDNF. Taken together, these data suggest that sucrose-induced alterations in glucose metabolism may contribute to a long-term decline in proBDNF and impaired recognition memory.
The Interplay between Finasteride-Induced Androgen Imbalance, Endoplasmic Reticulum Stress, Oxidative Stress, and Liver Disorders in Paternal and Filial Generation
Authors: Rzeszotek Sylwia , Kolasa Agnieszka , Pilutin Anna , Misiakiewicz-Has Kamila , Sielatycka Katarzyna , Wiszniewska Barbara
Published: October 2022

Finasteride (Fin) causes androgen imbalance by inhibiting the conversion of testosterone (T) to its more active metabolite, dihydrotestosterone (DHT). Androgen receptors (AR) are present (e.g., in hepatocytes), which have well-developed endoplasmic reticulum (ERet). Cellular protein quality control is carried out by ERet in two paths: (i) unfolded protein response (UPR) and/or (ii) endoplasmic reticulum associated degradation (ERAD). ERet under continuous stress can generate changes in the UPR and can direct the cell on the pathway of life or death. It has been demonstrated that genes involved in ERet stress are among the genes controlled by androgens in some tissues. Oxidative stress is also one of the factors affecting the functions of ERet and androgens are one of the regulators of antioxidant enzyme activity. In this paper, we discuss/analyze a possible relationship between androgen imbalance in paternal generation with ERet stress and liver disorders in both paternal and filial generation. In our rat model, hyperglycemia and subsequent higher accumulation of hepatic glycogen were observed in all filial generation obtained from females fertilized by Fin-treated males (F1:Fin). Importantly, genes encoding enzymes involved in glucose and glycogen metabolism have been previously recognized among UPR targets.
One-Pot Preparation of Imidazole-Ring-Modified Graphitic Carbon Nitride Nanozymes for Colorimetric Glucose Detection
Authors: Chen Yuanyuan , Gao Xueyou , Xue Hang , Liu Guohui , Zhou Yue , Peng Jian
Published: October 2022

Nanozymes are highly desired to overcome the shortcomings of natural enzymes, such as low stability, high cost and difficult storage during biosensing applications. Herein, by imitating the structure of natural enzymes, we propose a one-pot annealing process to synthesis imidazole-ring-modified graphitic carbon nitride (g-C3N4-Im) with enhanced peroxidase-like activity. g-C3N4-Im shows enhanced peroxidase-like activity by 46.5 times compared to unmodified g-C3N4. Furthermore, imidazole rings of g-C3N4-Im make it possible to anchor Cu(II) active sites on it to produce g-C3N4-Im-Cu, which shows a further increase in peroxidase-like activity by three times. It should be noted that the as-prepared g-C3N4-Im-Cu could show obvious peroxidase-like activity over a broad range of pH values and at a low temperature (5 °C). The ultrahigh peroxidase-like activity is attributed to the electronic effect of imidazole rings and the active sites of Cu(II) for ·OH production. Based on the enhanced peroxidase-like activity, a H2O2 and glucose biosensor was developed with a high sensitivity (limit of detection, 10 nM) and selectivity. Therefore, the biosensor shows potential for applications in diabetic diagnoses in clinical practice.
Hierarchical Ti-MOF Microflowers for Synchronous Removal and Fluorescent Detection of Aluminum Ions
Authors: Zhou Jianguo , Song Jieyao , Ma Guangqiang , Li Yongjian , Wei Yanan , Liu Fei , Zhou Hongjian
Published: October 2022

Bifunctional luminescence metal-organic frameworks with unique nanostructures have drawn ongoing attention for simultaneous determination and elimination of metal ions in the aqueous environment, but still remain a great challenge. In this work, three-dimensional hierarchical titanium metal-organic framework (Ti-MOF) microflowers were developed by a secondary hydrothermal method for not only highly sensitive and selective detection of Al(III), but also simultaneously efficient decontamination. The resulting Ti-MOF microflowers with a diameter of 5–6 μm consisted of nanorods with a diameter of ∼200 nm and a length of 1–2 μm, which provide abundant, surface active sites for determination and elimination of Al(III) ions. Because of their substantial specific surface area and superior fluorescence characteristics, Ti-MOF microflowers are used as fluorescence probes for quantitative determination of Al(III) in the aqueous environment. Importantly, the specific FL enhancement by Al(III) via a chelation-enhanced fluorescence mechanism can be utilized for selective and quantitative determination of Al(III). The Al(III) detection has a linear range of 0.4–15 µM and a detection limit as low as 75 nM. By introducing ascorbic acid, interference of Fe(III) can be avoided to achieve selective detection of Al(III) under various co-existing cations. It is noteworthy that the Ti-MOF microflowers exhibit excellent adsorption capacity for Al(III) with a high adsorption capacity of 25.85 mg g−1. The rapid adsorption rate is consistent with a pseudo-second order kinetic model. Ti-MOF is a promising contender as an adsorbent and a fluorescent chemical sensor for simultaneous determination and elimination of Al(III) due to its exceptional water stability, high porosity, and intense luminescence.
Interaction between Feet and Gaze in Postural Control
Authors: Bucci Maria Pia , Villeneuve Philippe
Published: October 2022

In the last decade, the number of postural studies in humans, in particular on deficits in postural control in patients, has increased. In the present article, we review studies on postural control with a particular focus on the role of foot and visual inputs in a quiet postural stance. A search and synthesis of articles identified through the electronic databases Scopus, Web of Science, EBSCO, PubMed, and ResearchGate published until 2021 was performed. The aim of this review is to provide reference values for further studies dealing with postural control in both healthy and pathologic subjects and to encourage the development of suitable protocols that take into account the specificity of the different systems responsible for controlling human balance.
Integration between Novel Imaging Technologies and Modern Radiotherapy Techniques: How the Eye Drove the Chisel
Authors: Francolini Giulio , Morelli Ilaria , Carnevale Maria Grazia , Grassi Roberta , Nardone Valerio , Loi Mauro , Valzano Marianna , Salvestrini Viola , Livi Lorenzo , Desideri Isacco
Published: October 2022

Introduction: Targeted dose-escalation and reduction of dose to adjacent organs at risk have been the main goal of radiotherapy in the last decade. Prostate cancer benefited the most from this process. In recent years, the development of Intensity Modulated Radiation Therapy (IMRT) and Stereotactic Body Radiotherapy (SBRT) radically changed clinical practice, also thanks to the availability of modern imaging techniques. The aim of this paper is to explore the relationship between diagnostic imaging and prostate cancer radiotherapy techniques. Materials and Methods: Aiming to provide an overview of the integration between modern imaging and radiotherapy techniques, we performed a non-systematic search of papers exploring the predictive value of imaging before treatment, the role of radiomics in predicting treatment outcomes, implementation of novel imaging in RT planning and influence of imaging integration on use of RT in current clinical practice. Three independent authors (GF, IM and ID) performed an independent review focusing on these issues. Key references were derived from a PubMed query. Hand searching and clinicaltrials.gov were also used, and grey literature was searched for further papers of interest. The final choice of papers included was discussed between all co-authors. Results: This paper contains a narrative report and a critical discussion of the role of new modern techniques in predicting outcomes before treatment, in radiotherapy planning and in the integration with systemic therapy in the management of prostate cancer. Also, the role of radiomics in a tailored treatment approach is explored. Conclusions: Integration between diagnostic imaging and radiotherapy is of great importance for the modern treatment of prostate cancer. Future clinical trials should be aimed at exploring the real clinical benefit of complex workflows in clinical practice.
Acoustic Radiation Force Impulse Elastography Assessment of Lymphoedema Tissue: An Insight into Tissue Stiffness
Authors: Sanderson Jennifer , Tuttle Neil , Laakso Liisa
Published: October 2022

Palpation remains essential for evaluating lymphoedema to detect subtle changes that may indicate progression. As palpation sense is not quantifiable, this study investigates the utility of ultrasound elastography to quantify stiffness of lymphoedema tissue and explore the influence of the pitting test on tissue stiffness. Fifteen women with unilateral arm lymphoedema were scanned using a Siemens S3000 Acuson ultrasound (Siemens, Germany) with 18 MHz and 9 MHz linear transducers to assess tissue structure and tissue stiffness with Acoustic Radiation Force Impulse elastography. Ninety sites were assessed, three on each of the lymphoedema-affected and contralateral unaffected arms. A subgroup of seven lymphoedema-affected sites included additional elastography imaging after a 60-s pitting test. Dermal tissue stiffness was greater than subcutaneous tissue stiffness regardless of the presence of pathology (p < 0.001). Lymphoedematous tissue exhibited a higher dermal to subcutaneous tissue stiffness ratio than contralateral sites (p = 0.005). Subgroup analysis indicated that the pitting test reduces dermal tissue stiffness (p = 0.018) and may alter the stiffness of the subcutaneous tissue layer. Elastography demonstrates potential as a complement to lymphoedema palpation assessment. The novel pre-test and post-pitting elastography imaging protocol yielded information representative of lymphoedema tissue characteristics that could not be ascertained from pre-test elastography images alone.
Penile Squamous Cell Carcinomas in Sub-Saharan Africa and Europe: Differential Etiopathogenesis
Authors: Manzotti Carolina , Chulo Laurina , López del Campo Ricardo , Trias Isabel , del Pino Marta , Saúde Ofélia , Basílio Iracema , Tchamo Nelson , Lovane Lucilia , Lorenzoni Cesaltina , Fernandes Fabiola , Saco Adela , Rodrigo-Calvo Maria Teresa , Marimon Lorena , Ismail Mamudo R. , Carrilho Carla , Ribera-Cortada Inmaculada , Ordi Jaume , Rakislova Natalia
Published: October 2022

Penile squamous cell carcinomas (PSCC) are classified by the World Health Organization into two categories based on their relationship with the human papillomavirus (HPV): HPV-associated and HPV-independent. We compared a cohort of PSCC from Mozambique, a sub-Saharan country in southeast Africa with a high prevalence of HPV and HIV infection, and Spain, a country in southwestern Europe with a low prevalence of HPV and HIV, to study the distribution of the etiopathogenic categories of these tumors in both sites. A total of 79 PSCC were included in the study (28 from Mozambique and 51 from Spain). All cases underwent HPV-DNA polymerase chain reaction (PCR) testing, genotyping, and immunohistochemistry for p16 and p53. Any PSCC showing either p16 overexpression or HPV-DNA in PCR analysis was considered HPV-associated. Overall, 40/79 (50.6%) tumors were classified as HPV-associated and 39 (49.4%) as HPV-independent. The two sites showed marked differences: 25/28 (89.3%) tumors from Mozambique and only 15/51 (29.4%) from Spain were HPV-associated (p < 0.001). HPV16 was the most frequent HPV type identified in 64.0% (16/25) of the HPV-associated tumors from Mozambique, and 60.0% (9/15) from Spain (p = 0.8). On average, patients from Mozambique were almost two decades younger than those from Spain (mean age 50.9 ± 14.9 and 69.2 ± 13.3, respectively [p < 0.001]). In conclusion, significant etiopathogenic differences between PSCC in Mozambique and Spain were observed, with a remarkably high prevalence of HPV-associated tumors in Mozambique and a relatively low prevalence in Spain. These data may have important consequences for primary prevention of PSCC worldwide.
Management of Bladder Cancer Patients with Clinical Evidence of Lymph Node Invasion (cN+)
Authors: Małkiewicz Bartosz , Gurwin Adam , Karwacki Jakub , Nagi Krystian , Knecht-Gurwin Klaudia , Hober Krzysztof , Łyko Magdalena , Kowalczyk Kamil , Krajewski Wojciech , Kołodziej Anna , Szydełko Tomasz
Published: October 2022

The purpose of this review is to present the current knowledge about the diagnostic and treatment options for bladder cancer (BCa) patients with clinically positive lymph nodes (cN+). This review shows compaction of CT and MRI performance in preoperative prediction of lymph node invasion (LNI) in BCa patients, along with other diagnostic methods. Most scientific societies do not distinguish cN+ patients in their guidelines; recommendations concern muscle-invasive bladder cancer (MIBC) and differ between associations. The curative treatment that provides the best long-term survival in cN+ patients is a multimodal approach, with a combination of neoadjuvant chemotherapy (NAC) and radical cystectomy (RC) with extended pelvic lymph node dissection (ePLND). The role of adjuvant chemotherapy (AC) remains uncertain; however, emerging evidence indicates comparable outcomes to NAC. Therefore, in cN+ patients who have not received NAC, AC should be implemented. The response to ChT is a crucial prognostic factor for cN+ patients. Recent studies demonstrated the growing importance of immunotherapy, especially in ChT-ineligible patients. Moreover, immunotherapy can be suitable as adjuvant therapy in selected cases. In cN+ patients, the extended template of PLND should be utilized, with the total resected node count being less important than the template. This review is intended to draw special attention to cN+ BCa patients, as the oncological outcomes are significantly worse for this group.
Impact of Patient, Surgical, and Perioperative Factors on Discharge Disposition after Radical Cystectomy
Authors: Cacciamani Giovanni E. , Lee Ryan S. , Sanford Daniel I. , Yip Wesley , Cai Jie , Miranda Gus , Daneshmand Siamak , Aron Monish , Djaladat Hooman , Gill Inderbir S. , Desai Mihir
Published: October 2022

Radical cystectomy (RC) is a complex procedure associated with lengthy hospital stays and high complication and readmission rates. We evaluated the impact of patient, surgical, and perioperative factors on discharge disposition following RC at a tertiary referral center. From 2012 to 2019, all bladder cancer patients undergoing RC at our institution were identified (n = 1153). Patients were classified based on discharge disposition: to home or to continued facility-based rehabilitation centers (CFRs, n = 180 (15.61%) patients). On multivariate analysis of patient factors only, age [Risk Ratio (RR): 1.07, p < 0.001)], single marital status (RR: 1.09, p < 0.001), and living alone prior to surgery (RR: 2.55, p = 0.004) were significant predictors of discharge to CFRs. Multivariate analysis of patient, surgical, and perioperative factors indicated age (RR: 1.09, p < 0.001), single marital status (RR: 3.9, p < 0.001), living alone prior to surgery (RR: 2.42, p = 0.01), and major post-operative (Clavien > 3) complications (RR: 3.44, p < 0.001) were significant independent predictors of discharge to CFRs. Of note, ERAS did not significantly impact discharge disposition. Specific patient and perioperative factors significantly impact discharge disposition. Patients who are older, living alone prior to surgery, and/or have a major post-operative complication are more likely to be discharged to CFRs after RC.
What Is the Role of Body Composition Assessment in HCC Management?
Authors: Radu Pompilia , Ebadi Maryam , Montano-Loza Aldo J. , Dufour Jean Francois
Published: October 2022

In the last decade, body composition (BC) assessment has emerged as an innovative tool that can offer valuable data concerning nutritional status in addition to the information provided by the classical parameters (i.e., body mass index, albumin). Furthermore, published data have revealed that different types of body composition are associated with different outcomes. For example, abnormalities of skeletal muscle, a common finding in cirrhotic and oncologic patients, are associated with poor outcome (i.e., high morbidity and high mortality). The disposition (visceral/subcutaneous adipose tissue) and radiodensity of adipose tissue proved to also be determinant factors for HCC outcome. Despite all the advantages, BC assessment is not part of the standard pre-therapeutic workup. The main reasons are the high heterogeneity of data, the paucity of prospective studies, the lack of a standard assessment method, and the interpopulation variation of BC. This paper aims to review the available evidence regarding the role of BC as a prognostic tool in the HCC population undergoing various therapies.
Immunomodulatory Effects of Stereotactic Body Radiotherapy and Vaccination with Heat-Killed Mycobacterium Obuense (IMM-101) in Patients with Locally Advanced Pancreatic Cancer
Authors: van ‘t Land Freek R. , Lau Sai P. , de Koning Willem , Klaase Larissa , Vink Madelief , van Krimpen Anneloes , Dumas Jasper , Vadgama Disha , Nuyttens Joost J. , Mustafa Dana A. M. , Stadhouders Ralph , Willemsen Marcella , Stubbs Andrew P. , Aerts Joachim G. , van Eijck Casper H. J.
Published: October 2022

Background: Patients with locally advanced pancreatic cancer (LAPC) are treated with chemotherapy. In selected cases, stereotactic body radiotherapy (SBRT) can be added to the regimen. We hypothesized that adding an adjuvant containing a heat-killed mycobacterium (IMM-101) to SBRT may lead to beneficial immuno-modulatory effects, thereby improving survival. This study aims to investigate the safety of adding IMM-101 to SBRT and to investigate the immuno-modulatory effects of the combination treatment in the peripheral blood of LAPC patients. Methods: LAPC patients were treated with SBRT (40 Gy) and six intradermal vaccinations of one milligram IMM-101. The primary endpoint was an observed toxicity rate of grade 4 or higher. Targeted gene-expression profiling and multicolor flow cytometry were performed for longitudinal immune-monitoring of the peripheral blood. Results: Twenty patients received study treatment. No treatment-related adverse events of grade 4 or higher occurred. SBRT/IMM-101 treatment induced a transient decrease in different lymphocyte subsets and an increase in CD14+CD16−CD11b+HLA−DRlow myeloid-derived suppressor cells. Importantly, treatment significantly increased activated ICOS+, HLA-DR+ and Ki67+PD1+ T and NK cell frequencies. This was not accompanied by increased levels of most inhibitory markers, such as TIM-3 and LAG-3. Conclusions: Combination therapy with SBRT and a heat-killed mycobacterium vaccine was safe and had an immune-stimulatory effect.
Activity and Structure of Nano-Sized Cobalt-Containing Systems for the Conversion of Lignin and Fuel Oil to Synthesis Gas and Hydrocarbons in a Microwave-Assisted Plasma Catalytic Process
Authors: Tsodikov Mark V. , Bukhtenko Olga V. , Naumkin Alexander V. , Nikolaev Sergey A. , Chistyakov Andrey V. , Konstantinov Grigory I.
Published: October 2022

In this study, we present the results of lignin and fuel oil conversion to hydrogen, synthesis gas, and liquid hydrocarbons in the presence of nano-sized cobalt-containing systems in a microwave-assisted plasma catalytic process. The deposition of a small amount of cobalt on lignin increases its microwave absorption capacity and provides plasma generation in the reaction zone. The role of Co-containing particles in the above catalytic reactions is probably to activate the carbon bonds of lignin, which substantially increases the microwave absorption capacity of the system as a whole. The subsequent use of the cobalt-containing residue of lignin conversion as a catalytic system and MWI-absorbing material results in active fuel oil pyrolysis in a plasma catalytic process to afford gaseous and liquid hydrocarbons. In the plasma catalytic pyrolysis, fuel oil conversion is probably accompanied by the conversion of the organic matter of the residue and agglomeration of cobalt oxide particles.
Metal Sulfide Photocatalysts for Hydrogen Generation: A Review of Recent Advances
Authors: Mamiyev Zamin , Balayeva Narmina O.
Published: October 2022

Metal-sulfide nanostructures have piqued the interest of researchers for decades due to their intriguing optoelectronic properties. Indeed, significant advances and improvements have been made in various fundamental aspects for cutting-edge applications, such as water splitting and hydrogen production. Furthermore, rising demand for low-dimensional materials due to lower material consumption and improved performance due to quantum size effects has spurred research on semiconducting metal sulfides. Consequently, size-controllable nanostructures with diverse morphologies have been fabricated and studied for potential applications. However, the photocatalytic hydrogen evolution rate is still limited mainly by fast recombination rate, poor solar energy utilization and lack of surface-active sites for H2 reduction. This review will highlight particularly recent findings in metal-sulfide-based photocatalysts for hydrogen evolution reactions, considering the swift development and excellent research in this field. Following a brief overview of fundamental properties, we will explore state-of-the-art strategies for enhancing H2 generation efficiencies over the pristine, heterostructured and co-catalayzed metal-sulfide photocatalysts.
Is Black Titania a Promising Photocatalyst?
Authors: Janczarek Marcin , Endo-Kimura Maya , Wang Kunlei , Wei Zhishun , Akanda Md Mahbub A. , Markowska-Szczupak Agata , Ohtani Bunsho , Kowalska Ewa
Published: October 2022

Five different (commercial and self-synthesized) titania samples were mixed with NaBH4 and then heated to obtain black titania samples. The change in synthesis conditions resulted in the preparation of nine different photocatalysts, most of which were black in color. The photocatalysts were characterized by various methods, including X-ray diffraction (XRD), diffuse reflectance spectroscopy (DRS), X-ray photoelectron spectroscopy (XPS), scanning transmission electron microscopy (STEM), photoacoustic and reverse-double beam photoacoustic spectroscopy (PAS/RDB-PAS). The photocatalytic activity was tested for oxidative decomposition of acetic acid, methanol dehydrogenation, phenol degradation and bacteria inactivation (Escherichia coli) under different conditions, i.e., irradiation with UV, vis, and NIR, and in the dark. It was found that the properties of the obtained samples depended on the features of the original titania materials. A shift in XRD peaks was observed only in the case of the commercial titania samples, indicating self-doping, whereas faceted anatase samples (self-synthesized) showed high resistance towards bulk modification. Independent of the type and degree of modification, all modified samples exhibited much worse activity under UV irradiation than original titania photocatalysts both under aerobic and anaerobic conditions. It is proposed that the strong reduction conditions during the samples’ preparation resulted in the partial destruction of the titania surface, as evidenced by both microscopic observation and crystallographic data (an increase in amorphous content), and thus the formation of deep electron traps (bulk defects as oxygen vacancies) increasing the charge carriers’ recombination. Under vis irradiation, a slight increase in photocatalytic performance (phenol degradation) was obtained for only four samples, while two samples also exhibited slight activity under NIR. In the case of bacteria inactivation, some modified samples exhibited higher activity under both vis and NIR than respective pristine titania, which could be useful for disinfection, cancer treatment and other purposes. However, considering the overall performance of the black titania samples in this study, it is difficult to recommend them for broad environmental applications.
A Systematic Approach to Study Complex Ternary Co-Promoter Interactions: Addition of Ir, Li, and Ti to RhMn/SiO2 for Syngas Conversion to Ethanol
Authors: Khatamirad Mohammad , Konrad Martin , Gentzen Manuel , Boscagli Chiara , Almer Christian , Arinchtein Aleks , Geske Michael , Rosowski Frank , Kraehnert Ralph
Published: October 2022

The direct conversion of synthesis gas could open up economically viable routes for the efficient production of ethanol. RhMn/SiO2 represents one of the most active systems reported thus far. Potential improvements were reported by added dopants, i.e., Ir, Ti, and Li. Yet, combining these elements leads to contradicting results, owing to the complexity of the interactions in a multi-promoted system. This complexity is often encountered in heterogeneous catalysis. We report a systematic data-driven approach for the assessment of complex multi-promoter interactions based on a combination of design-of-experiment, high-throughput experimentation, statistical analysis, and mechanistic assessment. We illustrate this approach for the system RhMn/SiO2 promoted with Ir, Li, and Ti. Using this approach, we investigate the impact of promoters’ interactions on a mechanistic level. Our analysis depicts the means to learn hidden correlations in the performance data and, additionally, high performance for ethanol yield for the RhMnIr/SiO2 catalyst. The method presented outlines an efficient way to also elucidate co-promoter interactions in other complex environments.
A Theoretical and Experimental Study for Enzymatic Biodiesel Production from Babassu Oil (Orbignya sp.) Using Eversa Lipase
Authors: Alexandre Jeferson Yves Nunes Holanda , Cavalcante Francisco Thálysson Tavares , Freitas Lara Matias , Castro Alyne Prudêncio , Borges Pedro Tavares , de Sousa Junior Paulo Gonçalves , Filho Manoel Nazareno Ribeiro , Lopes Ada Amelia Sanders , da Fonseca Aluisio Marques , Lomonaco Diego , de Sousa Rios Maria Alexsandra , Sousa dos Santos José Cleiton
Published: October 2022

A theoretical and experimental study was carried out on the biocatalytic production of babassu biodiesel through enzymatic hydroesterification. The complete hydrolysis of babassu oil was carried out using a 1:1 mass solution at 40 °C for 4 h using 0.4% of lipase from Thermomyces lanuginosus (TLL). Then, with the use of Eversa® Transform 2.0 lipase in the esterification step, a statistical design was used, varying the temperature (25–55 °C), the molar ratio between free fatty acids (FFAs) and methanol (1:1 to 1:9), the percentage of biocatalyst (0.1% to 0.9%), and the reaction time (1–5 h) using the Taguchi method. The ideal reaction levels obtained after the statistical treatment were 5 h of reaction at 40 °C at a molar ratio of 1:5 (FFAs/methanol) using 0.9% of the biocatalyst. These optimal conditions were validated by chromatographic analysis; following the EN 14103 standard, the sample showed an ester concentration of 95.76%. A theoretical study was carried out to evaluate the stability of Eversa with FFAs. It was observed in the molecular docking results that the ligands interacted directly with the catalytic site. Through molecular dynamics studies, it was verified that there were no significant conformational changes in the studied complexes. Theoretical and experimental results show the feasibility of this process.
Enhanced CO2 Photoreduction over Bi2Te3/TiO2 Nanocomposite via a Seebeck Effect
Authors: Lei Yiming , Jia Zewei , Hu Huilin , Liu Lequan , Ye Jinhua , Wang Defa
Published: October 2022

The activation of carbon dioxide (CO2) molecules and separation/transfer of photoinduced charge carriers are two crucial factors influencing the efficiency of CO2 photoreduction. Herein, we report a p-type Bi2Te3/commercial TiO2 (pBT/P25) nanocomposite for enhanced CO2 photoreduction. Upon light irradiation, a temperature gradient formed in pBT induces the Seebeck effect to build a thermoelectric field, which promotes the charge carriers’ separation/transfer. Additionally, pBT with a strong light absorption capacity generates the photothermal effect favoring the activation of CO2 molecules. In addition, the excellent electric conductivity and large work function render pBT an efficient cocatalyst for further improving the charge carriers’ separation/transfer. Owing to the synergistic enhancement effect of pBT on the activation of CO2 molecules and promotion of charge separation/transfer, we achieved the highest CO evolution rate over pBT(2)/P25 of 19.2 μmol·gcat−1·h−1, which was approximately 5.5 times that of bare P25. This work suggests that a thermoelectric material/semiconductor nanocomposite could be developed as an efficient photo-thermo-electro-chemical conversion system for enhanced CO2 reduction via promoting the charge carriers’ separation/transfer.
MCM-41-Type Mesoporous Silicas Modified with Alumina in the Role of Catalysts for Methanol to Dimethyl Ether Dehydration
Authors: Szczepanik Natalia , Kowalczyk Andrzej , Piwowarska Zofia , Chmielarz Lucjan
Published: October 2022

MCM-41-type mesoporous silicas were modified with alumina by the impregnation, co-condensation, and template ion-exchange (TIE) methods. The obtained materials were characterized with respect to their chemical composition (ICP-OES), textural parameters (low-temperature N2 sorption), structure (XRD), and surface acidity (NH3-TPD) and tested as catalysts of methanol to dimethyl ether (DME) dehydration in a flow microreactor system. The catalytic performance of the studied materials was analyzed with respect to their porous structure, as well as their density and the strength of their acid sites. It was shown that the performance of the studied catalysts depends on the contribution of the surface exposed aluminum species, as well as their aggregation. For the most active catalyst, the study of its catalytic stability under rection conditions was performed. It was shown that the catalyst can be effectively regenerated by the incineration of carbon deposits under air flow at 550 °C for 1 h.
KLF9 Aggravates Streptozotocin-Induced Diabetic Cardiomyopathy by Inhibiting PPARγ/NRF2 Signalling
Authors: Li Fangfang , Peng Jingfeng , Feng Hui , Yang Yiming , Gao Jianbo , Liu Chunrui , Xu Jie , Zhao Yanru , Pan Siyu , Wang Yixiao , Xu Luhong , Qian Wenhao , Zong Jing
Published: October 2022

Aims: Krüppel-like Factor 9 (KLF9) is a transcription factor that regulates multiple disease processes. Studies have focused on the role of KLF9 in the redox system. In this study, we aimed to explore the effect of KLF9 on diabetic cardiomyopathy. Methods and Results: Cardiac-specific overexpression or silencing of KLF9 in C57BL/6 J mice was induced with an adeno-associated virus 9 (AAV9) delivery system. Mice were also subjected to streptozotocin injection to establish a diabetic cardiomyopathy model. In addition, neonatal rat cardiomyocytes were used to assess the possible role of KLF9 in vitro by incubation with KLF9 adenovirus or small interfering RNA against KLF9. To clarify the involvement of peroxisome proliferator-activated receptors (PPARγ), mice were subjected to GW9662 injection to inhibit PPARγ. KLF9 was upregulated in the hearts of mice with diabetic cardiomyopathy and in cardiomyocytes. In addition, KLF9 overexpression in the heart deteriorated cardiac function and aggravated hypertrophic fibrosis, the inflammatory response and oxidative stress in mice with diabetic cardiomyopathy. Conversely, cardiac-specific silencing of KLF9 ameliorated cardiac dysfunction and alleviated hypertrophy, fibrosis, the cardiac inflammatory response and oxidative stress. In vitro, KLF9 silencing in cardiomyocytes enhanced inflammatory cytokine release and oxidative stress; KLF9 overexpression increased these detrimental responses. Moreover, KLF9 was found to regulate the transcription of PPARγ, which suppressed the expression and nuclear translocation of nuclear Factor E2-related Factor 2 (NRF2). In mice injected with a PPARγ inhibitor, the protective effects of KLF9 knockdown on diabetic cardiomyopathy were counteracted by GW9662 injection. Conclusions: KLF9 aggravates cardiac dysfunction, the inflammatory response and oxidative stress in mice with diabetic cardiomyopathy. KLF9 may become a therapeutic target for diabetic cardiomyopathy.
ATP7B-Deficient Hepatocytes Reveal the Importance of Protein Misfolding Induced at Low Copper Concentration
Authors: Charbonnier Peggy , Chovelon Benoît , Ravelet Corinne , Ngo Tuan Dung , Chevallet Mireille , Deniaud Aurélien
Published: October 2022

Copper is a transition metal essential for human life. Its homeostasis is regulated in the liver, which delivers copper to the whole body and excretes its excess outside the organism in the feces through the bile. These functions are regulated within hepatocytes, and the ATP7B copper transporter is central to making the switch between copper use and excretion. In Wilson disease, the gene coding for ATP7B is mutated, leading to copper overload, firstly, in the liver and the brain. To better understand the role of ATP7B in hepatocytes and to provide a smart tool for the development of novel therapies against Wilson disease, we used the CrispR/Cas9 tool to generate hepatocyte cell lines with the abolished expression of ATP7B. These cell lines revealed that ATP7B plays a major role at low copper concentrations starting in the micromolar range. Moreover, metal stress markers are induced at lower copper concentrations compared to parental cells, while redox stress remains not activated. As shown recently, the main drawback induced by copper exposure is protein unfolding that is drastically exacerbated in ATP7B-deficient cells. Our data enabled us to propose that the zinc finger domain of DNAJ-A1 would serve as a sensor of Cu stress. Therefore, these Wilson-like hepatocytes are of high interest to explore in more detail the role of ATP7B.
Spontaneous Pneumomediastinum in a 16-Year-Old Patient with SARS-CoV-2 Infection: A North-East Romanian Case
Authors: Filip Florin , Terteliu Baitan Monica , Caliman Sturdza Olga Adriana , Gheorghita Puscaselu Roxana , Filip Roxana
Published: October 2022

Spontaneous pneumomediastinum (SPM) associated with SARS-CoV-2 infection is a rare condition but can represent a medical emergency. It is probably related to alveolar damage secondary to SARS-CoV-2 infection, which allows air to escape in the surrounding lung tissue. Cough and airways’ barotrauma are also mentioned as contributing mechanisms. Treatment is generally conservative, but surgery may be required in severe cases. This paper presents the case of a 16-year-old girl with COVID-19-associated SPM who was treated conservatively in our department. The clinical course was favorable with resolution of respiratory symptoms and radiological (chest CT scan) image of pneumomediastinum. The patient was discharged 7 days after the confirmation of the initial SP diagnosis with appropriate treatment and recommendations for isolation. The sudden occurrence of chest pain and dyspnea should raise the suspicion of SPM in COVID-19 patients. Close surveillance and proper radiological monitoring are required in such cases. Treatment should be strictly individualized based on clinical course and radiological appearance.
Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5
Authors: Sharifi Simin , Dalir Abdolahinia Elaheh , Ghavimi Mohammad Ali , Dizaj Solmaz Maleki , Aschner Michael , Saso Luciano , Khan Haroon
Published: October 2022

Curcumin is an active ingredient isolated from Curcuma longa. It has several pharmacological effects, including anticancer, anti-inflammatory, and antioxidant effects. Due to its low bioavailability, chemical structure instability, and easy oxidation, the application of curcumin has been limited. In this study, to overcome these limitations, curcumin-loaded mesoporous silica nanoparticles (Cur-MSN) were prepared, and the anticancerous effect of Cur-MSNs on head and neck cancer cells, HN5, was investigated. Transmission electron microscopy (TEM) revealed rod-shaped mesoporous nanoparticles with average particle size smaller than 100 nm. Higher cytotoxicity of Cur-MSNs was seen in treated cancer cells compared with free curcumin. The expression of Bcl-2 was significantly reduced in the presence of Cur-MSNs compared to the control (untreated HN5 cells) (p < 0.05). A 3.43-fold increase in the Bax/Bcl-2 ratio was seen in Cur-MSNs treated HN5 cells at the IC50. Cur-MSNs increased intracellular reactive oxygen species (ROS) production. Based on these novel results, we suggest that Cur-MSNs offer efficacy for cancer treatment and future studies should further characterize their properties in various experimental cancer models.
A Case of Severe Hypoxia Caused by Phenazopyridine-Induced Methemoglobinemia: A near Fatal Event from Over-the-Counter Medication Use
Authors: KC Ojbindra , Subedi Ananta , Sharma Rakshya , Dahal Punya Hari , Koirala Manisha
Published: October 2022

Methemoglobinemia is a rare blood disorder characterized by the oxidation of heme iron from ferrous (Fe2+) to ferric (Fe3+) state, which increases oxygen affinity and impairs oxygen release to the tissue causing hypoxia. It can be congenital or acquired; however, most cases are acquired and caused by exogenous substances such as medications, chemicals, and environmental substances. Phenazopyridine is an over-the-counter urinary analgesic medication commonly used for symptomatic relief of dysuria and has been reported to cause methemoglobinemia. However, only a handful of cases of phenazopyridine-induced methemoglobinemia have been reported. We present a case of an 89-year-old female who presented with severe hypoxia, shortness of breath, headache, nausea, and dizziness caused by phenazopyridine-induced methemoglobinemia. She was found to have a methemoglobin level of 21.5% and was treated with methylene blue, leading to a rapid improvement of her symptoms. She was taking one over-the-counter phenazopyridine 200 mg tablet three times daily for two weeks for her chronic dysuria. This case highlights the need to have a high index of suspicion of phenazopyridine-induced methemoglobinemia in a patient presenting with unexplained shortness of breath with a history of phenazopyridine use as it could lead to severe methemoglobinemia with hypoxia that could potentially be fatal if not promptly diagnosed.
Highly Bioactive Elastomeric Hybrid Nanoceramics for Guiding Bone Tissue Regeneration
Authors: Chen Jing , Que Wenxiu , Lei Bo , Li Beibei
Published: October 2022

Conventional bioactive ceramic implants possess high osteogenic ability but exhibit poor machinability and brittleness, which limit their wide applications. In this study, we report an elastomeric machinable bioactive nanoceramic-based hybrid membrane that is formed by nanohydroxyapatite-reinforced hybrid matrix (poly(dimethylsilicone)-bioactive glass-poly(caprolactone) (nHA-PBP)) using a modified sol-gel process. The hybrid matrix is composed of elastomeric polydimethylsiloxane and bioactive glass nanogel. The effect of the nHA contents (0, 20, 30, 40 and 50 wt%) on the physicochemical structure and biomineralization activity of PBP hybrid membranes is investigated systematically. The results show that nHA-PBP hybrid membranes containing more than 20 wt% nHA exhibit the highest apatite-forming bioactivity due to the optimized hydroxyapatite crystalline phase. NHA-PBP implants with nHA also show good elastomeric mechanical behavior and foldable mechanical properties. Furthermore, the study of the in vitro cellular biocompatibility suggests that the nHA-PBP hybrid monoliths can enhance osteoblast (MC3T3-E1) attachment and proliferation. The biomimetic hybrid composition, crack-free monolith structure, and high biological activity of apatite formation make the nHA-PBP hybrid membrane a prospective candidate in the application of bone tissue regeneration.
Modeling of the Stress–Strain of the Suspensions of the Stators of High-Power Turbogenerators
Authors: Tretiak Oleksii , Kritskiy Dmitriy , Kobzar Igor , Sokolova Victoria , Arefieva Mariia , Tretiak Iryna , Denys Hromenko , Nazarenko Viacheslav
Published: October 2022

In the submitted scientific work, the existing types of stator fastening design of turbogenerators and the main causes of the stressed state of the stator suspensions are considered. A detailed calculation of the complex stressed state of the turbogenerator stator suspension was carried out for a number of electrical sheet steels, taking into consideration the unevenness of the heat distribution along the horizontal axis of the unit. It is proposed that the calculation of the mechanical stress is carried out by means of the mechanical and thermal calculation, coordinated with the electrical one. The possibility of replacing steel 38Х2Н2ВА with steel 34CrNiMo6 and 40NiCrMo7 is indicated, subject to compliance with GOST 8479-70 for the same strength group.