bluuebunny/crossref_metadata_2025 huggingface dataset:
Advancing Life Cycle Assessment of Sustainable Green Hydrogen Production Using Domain-Specific Fine-Tuning by Large Language Models Augmentation
Authors: Chen Yajing , Liebau Urs , Guruprasad Shreyas Mysore , Trofimenko Iaroslav , Minke Christine
Published: November 2024

Assessing the sustainable development of green hydrogen and assessing its potential environmental impacts using the Life Cycle Assessment is crucial. Challenges in LCA, like missing environmental data, are often addressed using machine learning, such as artificial neural networks. However, to find an ML solution, researchers need to read extensive literature or consult experts. This research demonstrates how customised LLMs, trained with domain-specific papers, can help researchers overcome these challenges. By starting small by consolidating papers focused on the LCA of proton exchange membrane water electrolysis, which produces green hydrogen, and ML applications in LCA. These papers are uploaded to OpenAI to create the LlamaIndex, enabling future queries. Using the LangChain framework, researchers query the customised model (GPT-3.5-turbo), receiving tailored responses. The results demonstrate that customised LLMs can assist researchers in providing suitable ML solutions to address data inaccuracies and gaps. The ability to quickly query an LLM and receive an integrated response across relevant sources presents an improvement over manually retrieving and reading individual papers. This shows that leveraging fine-tuned LLMs can empower researchers to conduct LCAs more efficiently and effectively.
Deep Learning-Based In Situ Micrograph Synthesis and Augmentation for Crystallization Process Image Analysis
Authors: Li Muyang , Yao Tuo , Liu Jian , Liu Ziyi , Gao Zhenguo , Gong Junbo
Published: November 2024

Deep learning-based in situ imaging and analysis for crystallization process are essential for optimizing product qualities, reducing experimental costs through real-time monitoring, and controlling the process. However, large and high-quality annotated datasets are required to train accurate models, which are time consuming. Therefore, we proposed a novel methodology that applied image synthesis neural networks to generate virtual information-rich images, enabling efficient and rapid dataset expansion while simultaneously reducing annotation costs. Experiments were conducted on the L-alanine crystallization process to obtain process images and to validate the proposed workflow. The proposed method, aided by interpolation augmentation and data warping augmentation to enhance data richness, utilized only 25% of the training annotations, consistently segmenting crystallization process images comparable to those models utilizing 100% of the training data annotations, achieving an average precision of nearly 98%. Additionally, based on the analysis of Kullback–Leibler divergence, the proposed method demonstrated excellent performance in extracting in situ information regarding aspect ratios and crystal size distributions during the crystallization process. Moreover, its ability to leverage expert labels with a four-fold enhanced efficiency holds great potential for advancing various applications in crystallization processes.
Lie Group Classification of a Class of Variable Coefficient Boiti–Leon–Manna–Pempinelli Equations
Authors: Sophocleous Christodoulos
Published: November 2024

The Boiti–Leon–Manna–Pempinelli (BLMP) equation with coefficients being functions of time is considered. Since the coefficient functions are arbitrary, we have a class of BLMP equations. Symmetry analysis is carried out for this class. We derive the equivalence group admitted by the class and we present the enhanced Lie group classification. Lie symmetries are used to construct similarity reductions. Reduction operators that are not equivalent to Lie ones are also constructed.
Real-Time Run-Off-Road Risk Prediction Based on Deep Learning Sequence Forecasting Approach
Authors: Chen Yunteng , Wei Lijun , Bao Qiong , Zhang Huansong
Published: November 2024

Driving risk prediction is crucial for advanced driving technologies, with deep learning approaches leading the way in driving safety analysis. Current driving risk prediction methods typically establish a mapping between driving features and risk statuses. However, status prediction fails to provide detailed risk sequence information, and existing driving safety analyses seldom focus on run-off-road (ROR) risk. This study extracted 660 near-roadside lane-changing samples from the high-D natural driving dataset. The performance of sequence and status prediction for ROR risk was compared across five mainstream deep learning models: LSTM, CNN, LSTM-CNN, CNN-LSTM-MA, and Transformer. The results indicate the following: (1) The deep learning approach effectively predicts ROR risk. The Macro F1 Score of sequence prediction significantly surpasses that of status prediction, with no notable difference in efficiency; (2) Sequence prediction captures risk evolution trends, such as increases, turns, and declines, providing more comprehensive safety information; (3) The presence of surrounding vehicles significantly impacts lane change duration and ROR risk. This study offers new insights into the quantitative research of ROR risk, demonstrating that risk sequence prediction is superior to status prediction in multiple aspects and can provide theoretical support for the development of roadside safety.
The Impact of the Position of the Humeral Head Relative to the Glenoid on Iatrogenic Fractures During Shoulder Dislocation Reduction
Authors: Gunsoy Zeki , Oguzkaya Sinan , Sayer Gokhan , Golgelioglu Fatih , Dinc Mustafa , Misir Abdulhamit
Published: November 2024

Background and Objectives: The aim of this study was to investigate the relationship between the position of the humeral head relative to the glenoid and the occurrence of iatrogenic surgical neck fractures of the humerus during anterior glenohumeral dislocation reductions. Materials and Methods: Patients with first-time anterior shoulder dislocations without generalized joint hyperlaxity were included. The humeral head displacement ratio was calculated as the distance between the medial border of the humeral head and the anterior glenoid rim divided by the diameter of the humeral head. Demographic data, concomitant tuberculum majus fractures, and the deltoid tuberosity index were recorded. Patients were divided into three groups: Group 1 (iatrogenic fracture development during closed reduction (CR)), Group 2 (failed CR), and Group 3 (successful reduction without iatrogenic fracture). Complicated dislocations were included in Groups 1 and 2, while uncomplicated dislocations were those in Group 3. Results: The study included 89 patients with a mean age of 46.44 ± 19.02 years (64 males, 25 females). Concomitant tuberculum majus fractures occurred in 37 (41.6%) cases. Iatrogenic surgical neck fractures occurred in 10 patients (Group 1), and CR was unsuccessful in 8 patients (Group 2), totaling 18 cases of complicated dislocations. Reduction without iatrogenic fracture was achieved in 71 cases (Group 3). The mean humeral head displacement ratio was higher in the complex dislocation group (92.91 ± 15.34 vs. 75.01 ± 13.80; p < 0.001). Complicated dislocations were more frequent in patients with tuberculum majus fractures (p = 0.031). Subgroup analysis showed higher humeral head displacement ratios in Groups 1 and 2 compared to Group 3 (p = 0.010 and p = 0.06, respectively). Tuberculum majus fractures were more frequent in Group 1 compared to Group 3 (p = 0.013), with no significant difference between Groups 2 and 3. Conclusions: In patients experiencing first-time traumatic anterior shoulder dislocations, a greater medial displacement of the humeral head relative to the glenoid rim significantly increases the risk of iatrogenic humeral fractures and the likelihood of unsuccessful closed reduction attempts.
Hemi-Percutaneous Epiphysiodesis Using Transphyseal Screws at Lateral Proximal Tibias After Epiphyseal Fusion of Distal Phalanges in the Hand Results in Undercorrection of Genu Varum
Authors: Ko Kyung Rae , Shim Jae Woo , Shim Jong Sup , Kim Dong Suk , Lee Soonchul
Published: November 2024

Background and Objectives: To investigate postoperative courses after hemi-percutaneous epiphysiodesis using transphyseal screws (PETS) for genu varum. We especially focused on the degree of skeletal maturation that results in undercorrection. Materials and Methods: We identified patients with idiopathic genu varum treated with hemi-PETS at the proximal tibia and followed-up to the completion of skeletal maturation. The acceptable correction was defined as the (1) final deformity < varus 1.0° or (2) final correction angle obtained by surgery (f-CA) > mean value of preoperative deformity. Results: In our cohort of 29 patients and their 29 lower limbs (one side was randomly selected in bilateral cases), the mean hip–knee–ankle (HKA) alignment was varus 6.5 ± 1.3° at the time of hemi-PETS. The mean f-CA was 5.8 ± 2.0° with a rebound of 0.3 ± 1.0°. Ten patients showed the finding of partial or complete fusion of the epiphysis of distal phalanges in the hand at the time of hemi-PETS (the fusion group, FG). Their f-CA was 4.0 ± 1.9° (with preoperative deformity of 6.9 ± 1.4°), which was significantly smaller than that (6.7 ± 1.3°, p = 0.001) of remaining 19 patients (the open group, OG). The acceptable correction was obtained in all 19 patients of the OG. Otherwise, it was obtained in two patients in the FG (p < 0.001). The other two patients in the FG preoperatively showed a complete epiphyseal fusion of the distal phalanges in the hand, and their f-CA was 0.7 and 1.1°, respectively. Conclusions: The degree of skeletal maturation corresponding to epiphyseal fusion of distal phalanges in the hand results in undercorrection after the hemi-PETS performed at the proximal tibia for genu varum.
Biostimulant-Based Molecular Priming Improves Crop Quality and Enhances Yield of Raspberry and Strawberry Fruits
Authors: Kazakov Petar , Alseekh Saleh , Ivanova Valentina , Gechev Tsanko
Published: November 2024

Background/Objectives: The biostimulant SuperFifty, produced from the brown algae Ascophyllum nodosum, can improve crop quality and yield and mitigate stress tolerance in model and crop plants such as Arabidopsis thaliana, pepper, and tomato. However, the effect of SuperFifty on raspberries and strawberries has not been well studied, especially in terms of nutritional properties and yield. The aim of this study was to investigate the effect of SuperFifty on the quality and quantity of raspberry and strawberry fruits, with a focus on metabolic composition and essential elements, which together determine the nutritional properties and total yield of these two crops. Methods: Metabolome analysis was performed by liquid chromatography–mass spectrometry analysis (LC-MS), and essential elements analysis was performed by inductively coupled plasma-mass spectrometry (ICP-MS). Results: Here, we demonstrate that SuperFifty increases the fruit size of both raspberries and strawberries and enhances the yield in these two berry crops by 42.1% (raspberry) and 33.9% (strawberry) while preserving the nutritional properties of the fruits. Metabolome analysis of 100 metabolites revealed that antioxidants, essential amino acids, organic acids, sugars, and vitamins, such as glutathione, alanine, asparagine, histidine, threonine, serine, tryptophan, sucrose, citric acid, pantothenic acid (vitamin B5), as well as other primary metabolites, remain the same in the SuperFifty-primed fruits. Secondary metabolites, such as caffeic acid, p-coumaric acid, kaempferol, and quercetin, also maintained their levels in the SuperFifty-primed fruits. Analysis of essential elements demonstrated that elements important for human health, such as Zn, Mn, Fe, B, Cu, K, and Ca, maintain the same levels in the raspberry and strawberry fruits obtained from the biostimulant-primed plants. Magnesium, an important element known as a co-factor in many enzymatic reactions related to both plant physiology and human health, increased in both raspberry and strawberry fruits primed with SuperFifty. Finally, we discuss the potential financial and health benefits of the SuperFifty-induced priming for both growers and consumers. Conclusions: We demonstrate that SuperFifty significantly enhances the yield of both raspberries and strawberries, improves the marketable grade of the fruits (larger and heavier fruits), and enhances the nutritional properties by elevating Mg content in the fruits. Altogether, this biostimulant-induced molecular priming offers an environmentally friendly, efficient, and sustainable way to enhance the yield and quality of berry crops, with clear benefits to both berry producers and customers.
Enteroviruses, Respiratory Syncytial Virus and Seasonal Coronaviruses in Influenza-like Illness Cases in Nepal
Authors: Shrestha Sanjaya K. , Shrestha Jasmin , Shrestha Binob , Strand Tor A. , Dudman Susanne , Andreassen Ashild K. , Shrestha Shree Krishna , Bastola Anup , Pandey Prativa , Fernandez Stefan
Published: November 2024

Acute respiratory infection is one of the leading causes of morbidity and mortality among children in low- and middle-income countries. Due to limited diagnostic capability, many respiratory pathogens causing influenza-like illness go undetected. This study aims to detect enterovirus, respiratory syncytial virus, seasonal coronavirus and respiratory pathogens other than influenza in patients with influenza-like illness. A total of 997 (54.3%) respiratory samples (collected in the years 2016–2018) were randomly selected from 1835 influenza-negative samples. The xTAG Respiratory Viral Panel (RVP) FAST v2 panel was used to detect respiratory pathogens including enterovirus/rhinovirus (EV/RV), respiratory syncytial virus (RSV) and seasonal coronavirus (HKU1, OC43, NL63 and 229E). A total of 78.7% (785/997) were positive for respiratory viruses. Of these viruses, EV/RV was detected in 36.3% (362/997), which is the highest number, followed by RSV in 13.7% (137/997). The seasonal coronaviruses HKU1 and OC43 (1.5%, 15/997), NL63 (1.2%, 12/997) and 229E (1%, 10/997) were also detected. The EV/RV-positive samples were sequenced, of which 16.7% (5/30) were confirmed as EVs and were identified as coxsackievirus (CV) types CVB5, CVB3, CV21 and CVB2. The findings of this study highlight the importance of strengthening influenza-like illness surveillance programs in the region by including other respiratory viruses in their scope besides seasonal human influenza viruses.
Data-Supported Prediction of Surface Settlement Behavior on Opencast Mine Dumps Using Satellite-Based Radar Interferometry Observations
Authors: Benndorf Jörg , Merkel Natalie , John Andre
Published: November 2024

To ensure the safe repurposing of post-mining landscapes, understanding and managing geotechnical risks, particularly ground movements such as settlements on opencast mining dump surfaces, is critical. Satellite-based radar interferometry (InSAR) technology offers highly detailed data on vertical ground movements with a high spatial and temporal resolution. By combining a data-driven approach, using InSAR-generated high-resolution datasets, with model-driven methods such as inverse modeling and classic time–settlement models, the efficient monitoring and prediction of opencast mine dump settlements can be achieved. This dual approach—leveraging advanced data analysis tools and precise modeling—yields valuable insights into spatial settlement behavior. In particular, classic time–settlement models are applied to the InSAR data through least square regression and Taylor approximation. The integration of both approaches enables the more robust, data-validated forecasts of key geotechnical indicators, such as the time to settlement stabilization and the expected maximum settlement over large areas. An application at a mine in central Germany illustrates the method by generating spatial predictions of the settlement behavior over more than 200 ha. In general, the results provide a comprehensive dataset for investigating other factors influencing the settlement behavior of opencast mine dumps.
Evaluation of APTES-Functionalized Zinc Oxide Nanoparticles for Adsorption of CH4 and CO2
Authors: Montejo-Mesa Luis A. , Díaz-García Alicia M. , Cavalcante Celio L. , Vilarrasa-García Enrique , Rodríguez-Castellón Enrique , Ballesteros-Plata Daniel , Autié-Castro Giselle I.
Published: November 2024

ZnO nanoparticles functionalized with APTES were obtained to evaluate their CH4 and CO2 adsorption at 298 K in a range between 0 and 10 bar. First, ZnO nanoparticles were obtained by a precipitation method and subsequently coated with (3-aminopropyl)triethoxysilane (APTES). As a preliminary study, the results were compared with previously reported naked nanoparticles in order to evaluate the influence of APTES coating on CO2 selectivity. UV-Vis, FT-IR spectroscopy, TGA, XRD, TEM/EDX, XPS and N2 adsorption at 77 K were used to characterize the evaluated material. It was observed that the amount of gas adsorbed on the surface of the nanostructure was very small in comparison with other materials traditionally used for this purpose but slightly higher than those obtained in naked nanoparticles evaluated in previous studies. The affinity of CO2 for the amines groups of the APTES ligand was also discussed.
Therapeutic Potential of Ramalin Derivatives with Enhanced Stability in the Treatment of Alzheimer’s Disease
Authors: Kim Tai Kyoung , Hong Ju-Mi , Kim Jaewon , Kim Kyung Hee , Han Se Jong , Kim Il-Chan , Oh Hyuncheol , Jo Dong-Gyu , Yim Joung Han
Published: November 2024

Alzheimer’s disease (AD) remains a significant public health challenge with limited effective treatment options. Ramalin, a compound derived from Antarctic lichens, has shown potential in the treatment of AD because of its strong antioxidant and anti-inflammatory properties. However, its instability and toxicity have hindered the development of Ramalin as a viable therapeutic agent. The primary objective of this study was to synthesize and evaluate novel Ramalin derivatives with enhanced stabilities and reduced toxic profiles, with the aim of retaining or improving their therapeutic potential against AD. The antioxidant, anti-inflammatory, anti-BACE-1, and anti-tau activities of four synthesized Ramalin derivatives (i.e., RA-Hyd-Me, RA-Hyd-Me-Tol, RA-Sali, and RA-Benzo) were evaluated. These derivatives demonstrated significantly improved stabilities compared to the parent compound, with RA-Sali giving the most promising results. More specifically, RA-Sali exhibited a potent BACE-1 inhibitory activity and effectively reduced tau phosphorylation, a critical factor in AD pathology. Despite exhibiting reduced antioxidant activities compared to the parent compound, these derivatives represent a potential multi-targeted approach for AD treatment, marking a significant step forward in the development of stable and effective AD therapeutics.
Correction: Khan et al. Molecular Profiling, Characterization and Antimicrobial Efficacy of Silver Nanoparticles Synthesized from Calvatia gigantea and Mycena leaiana Against Multidrug-Resistant Pathogens. Molecules 2023, 28, 6291
Authors: Khan Sayab , Fiaz Muhammad , Alvi Iqbal Ahmad , Ikram Muhammad , Yasmin Humaira , Ahmad Junaid , Ullah Amin , Niaz Zeeshan , Hayat Shubana , Ahmad Ajaz , Kaushik Prashant , Farid Arshad
Published: November 2024

“Iqbal Ahmad Alvi” and “Muhammad Ikram” were not included as authors in the original publication [...]
Beneficial Effects of Epigallocatechin Gallate in Preventing Skin Photoaging: A Review
Authors: Sun Jiaqiang , Jiang Yuelu , Fu Jing , He Linlin , Guo Xinmiao , Ye Hua , Yin Cuiyuan , Li Hongbo , Jiang Heyuan
Published: November 2024

Skin photoaging, primarily caused by ultraviolet (UV) radiation, leads to skin metabolic disorders, which have adverse psychological and physiological effects on individuals. However, traditional medications for repairing skin photoaging cause side effects. Natural bioactive compounds have been shown to prevent and treat skin photoaging with fewer side effects. Epigallocatechin gallate (EGCG), the main substance in tea polyphenols, is a natural bioactive compound with a range of properties. This review summarizes the beneficial effects and mechanisms of EGCG, as well as the application forms of EGCG in repairing photoaged skin. Results indicated that EGCG has repair effects, including improving elasticity, enhancing moisturization, inhibiting damage, and reducing pigmentation of photoaged skin. It has also been demonstrated that EGCG delivery systems, modified EGCG, and combinations with other bioactive substances could be used for repairing photoaged skin due to its poor stability and low bioavailability. EGCG effectively repairs various types of skin damage caused by UV radiation while maintaining normal skin structure and function. It is, therefore, an effective candidate for repairing photoaged skin. These results could provide references for the development and application of EGCG products for the treatment of photoaged skin.
Enhanced Gallium Extraction Using Silane-Modified Mesoporous Silica Synthesized from Coal Gasification Slag
Authors: Yang Shiqiao , Fan Guixia , Ma Lukuan , Wei Chao , Li Peng , Cao Yijun , Teng Daoguang
Published: November 2024

This study presents an innovative approach to utilize coal gasification coarse slag (CGCS) for efficient and low-cost gallium extraction. Using a one-step acid leaching process, mesoporous silica with a surface area of 258 m2/g and a pore volume of 0.15 cm3/g was synthesized. The properties of CGCS before and after acid leaching were characterized through SEM, FTIR, XRD, and BET analyses, with optimal conditions identified for maximizing specific surface area and generating saturated silanol groups. The prepared mesoporous silica demonstrated a 99% Ga(III) adsorption efficiency. Adsorption conditions were optimized, and adsorption kinetics, isotherms, and competitive adsorption behaviors were evaluated. Competitive adsorption with vanadium suggests potential application in Ga(III) extraction from vanadium-rich waste solutions. Furthermore, the recyclability of both the acid and adsorbent was explored, with the adsorbent maintaining over 85% adsorption efficiency after five cycles. The adsorption mechanism was further elucidated through SEM-EDS, XPS, and FTIR analyses. This work not only advances resource recovery from industrial waste but also offers a sustainable method for gallium extraction with industrial applications.
A Comparison of the Impacts of Different Drying Methods on the Volatile Organic Compounds in Ginseng
Authors: Xiang Yun , Zou Manshu , Ou Feilin , Zhu Lijun , Xu Yingying , Zhou Qingqing , Lei Chang
Published: November 2024

Ginseng (Panax ginseng C. A. Meyer) is a valuable plant resource which has been used for centuries as both food and traditional Chinese medicine. It is popular in health research and markets globally. Fresh ginseng has a high moisture content and is prone to mold and rot, reducing its nutritional value without proper preservation. Drying treatments are effective for maintaining the beneficial properties of ginseng post-harvest. In this study, we investigated the effects of natural air drying (ND), hot-air drying (HAD), vacuum drying (VD), microwave vacuum drying (MVD), and vacuum freeze drying (VFD) on volatile organic compounds (VOCs) in ginseng. The results showed that the MVD time was the shortest, followed by the VFD, VD, and HAD times, whereas the ND time was the longest, but the VFD is the most beneficial to the appearance and color retention of ginseng. A total of 72 VOCs were obtained and 68 VOCs were identified using the five drying methods based on gas chromatography–ion mobility spectrometry (GC-IMS) technology, including 23 aldehydes, 19 alkenes, 10 alcohols, 10 ketones, 4 esters, 1 furan, and 1 pyrazine, and the ND method was the best for retaining VOCs. GC-IMS fingerprints, principal component analysis (PCA), Euclidean distance analysis, partial least squares discriminant analysis (PLS-DA), and cluster analysis (CA) can distinguish ginseng from different drying methods. A total of 29 VOCs can be used as the main characteristic markers of different drying methods in ginseng. Overall, our findings provide scientific theoretical guidance for optimizing ginseng’s drying methods, aromatic health effects, and flavor quality research.
Using a Game to Educate About Sustainable Development
Authors: Senka Gita , Tramonti Michela , Dochshanov Alden Meirzhanovich , Jesmin Triinu , Terasmaa Jaanus , Tsalapatas Hariklia , Heidmann Olivier , Caeiro-Rodriguez Manuel , Vaz de Carvalho Carlos
Published: November 2024

Sustainable natural resource management is a high priority in the 21st century as it plays a crucial role in mitigating climate change and preventing some of its consequences like loss of biodiversity, land degradation, desertification, and the exhaustion of natural resources. This concern is reflected in the United Nations Sustainable Development Goals (SDGs), which emphasize, among other factors, sustainable cities and communities, responsible production and consumption, and climate action. Achieving sustainable natural resource management begins with raising awareness and educating the next generation. Therefore, it is essential to develop educational initiatives that prepare young people to become responsible and proactive adults in promoting environmental sustainability across industries and communities. Additionally, these initiatives should develop critical and analytical thinking skills, nurture innovative mindsets for creating environmentally sound solutions, and enhance the ability to collaborate within multidisciplinary teams. The NATURE project addressed these needs by designing and developing a serious educational game that fosters this set of skills. The results of the pilot testing show that the game is an effective tool and contributes to the education and awareness of the younger generation.
The Role of Neuropeptide Y in the Pathogenesis of Alzheimer’s Disease: Diagnostic Significance and Neuroprotective Functions
Authors: Shapovalova Ksenia , Zorkina Yana , Abramova Olga , Andryushchenko Alisa , Chekhonin Vladimir , Kostyuk Georgy
Published: November 2024

Background. Alzheimer’s disease (AD) is one of the most common neurodegenerative diseases. It has been suggested that the factors that cause pathologic changes and lead to the development of AD may also include changes in certain neuropeptides. The implication of the neuropeptide (NPY) in the pathogenesis of AD and its potential therapeutic role is possible due to the following properties: involvement in adult neurogenesis, regulatory effects on the immune system, the inhibition of potential-dependent Ca2+ channels, and the reduction in glutamate excitotoxicity. The aim of our review was to summarize recent data on the role of NPY in AD development and to explore its potential as a biomarker and a possible therapeutic target. Materials and methods. We performed a systematic review of studies, for which we search using the keywords “Alzheimer’s disease and neuropeptide Y”, “Alzheimer’s disease and NPY”, “AD and NPY”, “Neuropeptide Y and Neurodegenerative disease”. Nineteen articles were included in the review. Results. The NPY levels in cerebrospinal fluid and plasma have been found to be reduced or unchanged in AD patients; however, these findings need to be confirmed in more recent studies. Data obtained in transgenic animal models support the role of NPY in AD pathogenesis. The neuroprotective effects of NPY have been demonstrated in vitro and in vivo in AD models. Conclusion. The findings may open new possibilities for using NPY as a diagnostic marker to detect AD at earlier stages of the disease or as a potential therapeutic target due to its neuroprotective properties.
Painful Legs and Moving Toes Syndrome: Case Report and Review
Authors: Tsalta-Mladenov Mihael , Dimitrova Vladina , Andonova Silva
Published: November 2024

Introduction: Painful legs and moving toes (PLMT) syndrome is a rare movement disorder characterized by defuse lower limb neuropathic pain and spontaneous abnormal, involuntary toe movements. Objective: The objective was to present a rare case of PLMT syndrome with a triggering area in an adult patient due to multilevel discogenic pathology, to make a thorough review of this disorder and to provide a practical approach to its management. Case presentation: A 59-years-old male was admitted to the neurology ward with symptoms of defuse pain in the lower-back and the right leg accompanied by involuntary movements for the right toes intensified by tactile stimulation in the right upper thigh. Magnetic resonance imaging (MRI) revealed a multilevel discogenic pathology of the lumbar and cervical spine, with myelopathy at C5-C7 level. A medication with Pregabalin 300 mg/daily significantly improved both the abnormal toe movements and the leg pain. The clinical effect was constant during the 90-day follow-up without any adverse effects. Conclusion: Painful legs and moving toes (PLMT) is a condition that greatly affects the quality of life of patients, but which still remains less known by clinicians. Spontaneous resolution is rare, and oral medications are the first-line treatment. Pregabalin is a safe and effective treatment option for PLMT that should be considered early for the management of this condition. Other medication interventions, such as botulinum toxin injections, spinal blockade, or non-pharmacological treatment options like spinal cord stimulation, and surgical decompressions, are also recommended when the conservative treatment is ineffective in well-selected patients.
Ethanolamine and Vinyl–Ether Moieties in Brain Phospholipids Modulate Behavior in Rats
Authors: Nasrin MST Zenika , Kikuchi Shuhei , Uchimura Yasuhiro , Yoshioka Mina , Morita Shin-ya , Kobayashi Tomoya , Kinoshita Yusuke , Furusho Yoshio , Tamiaki Hitoshi , Yanagisawa Daijiro , Udagawa Jun
Published: November 2024

Plasmalogens are brain-enriched phospholipids with a vinyl–ether bond at the sn-1 position between the glycerol backbone and the alkyl chain. Previous studies have suggested that plasmalogens modulate locomotor activity, anxiety-like behavior, and cognitive functions in rodents; however, the specific moieties contributing to behavioral regulation are unknown. In this study, we examined the behavioral modulation induced by specific phospholipid moieties. To confirm the permeability of phospholipids in injected liposomes, we measured the fluorescence intensity following intravenous injection of liposomes containing ATTO 740-labeled dioleoylphosphatidylethanolamine. Then, we compared the behavioral effects following injection of liposomes composed of egg phosphatidylcholine (PC) and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (PE 18:0/22:6), PC 18:0/22:6, 1-(1Z-octadecenyl)-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (PE P-18:0/22:6), or PC P-18:0/22:6, into the tail vein of male rats. The time spent in the central region of the open field was significantly reduced after injection of PE 18:0/22:6, harboring an ester bond at sn-1 compared to controls. Furthermore, the discrimination ratio in the novel object recognition test was significantly higher in PC 18:0/22:6 compared to PE 18:0/22:6, suggesting that the substitution of ethanolamine with choline can enhance recognition memory. We demonstrate that the structures of the sn-1 bond and the hydrophilic moiety in the phospholipids can modulate exploratory behaviors and recognition memory in rodents.
The Effect of Vitamin D Supplementation Post COVID-19 Infection and Related Outcomes: A Systematic Review and Meta-Analysis
Authors: Sartini Marina , Del Puente Filippo , Carbone Alessio , Schinca Elisa , Ottria Gianluca , Dupont Chiara , Piccinini Carolina , Oliva Martino , Cristina Maria Luisa
Published: November 2024

Background: Vitamin D’s role in COVID-19 management remains controversial. This meta-analysis aimed to evaluate the efficacy of vitamin D supplementation in patients with SARS-CoV-2 infection, focusing on mortality, intensive care unit (ICU) admissions, intubation rates, and hospital length of stay (LOS). Methods: A systematic review of PubMed/MEDLINE, Scopus, Cochrane, and Google Scholar databases was conducted. Randomized controlled trials (RCTs) and analytical studies investigating vitamin D supplementation in COVID-19 patients were included. The meta-analysis was performed using STATA MP 18.5, employing random-effect or fixed-effect models based on heterogeneity. Results: Twenty-nine studies (twenty-one RCTs, eight analytical) were analyzed. Vitamin D supplementation significantly reduced ICU admissions (OR = 0.55, 95% CI: 0.37 to 0.79) in RCTs and analytical studies (OR = 0.35, 95% CI: 0.18 to 0.66). Intubation rates were significantly reduced in RCTs (OR = 0.50, 95% CI: 0.27 to 0.92). Mortality reduction was significant in analytical studies (OR = 0.45, 95% CI: 0.24 to 0.86) but not in RCTs (OR = 0.80, 95% CI: 0.61 to 1.04). Subgroup analyses revealed more pronounced effects in older patients and severe COVID-19 cases. LOS showed a non-significant reduction (mean difference = −0.62 days, 95% CI: −1.41 to 0.18). Conclusions: This meta-analysis suggests potential benefits of vitamin D supplementation in COVID-19 patients, particularly in reducing ICU admissions. However, the evidence varies across outcomes and patient subgroups. Discrepancies between RCTs and analytical studies highlight the need for further large-scale, well-designed trials accounting for baseline vitamin D status, standardized supplementation protocols, and patient characteristics to inform clinical guidelines for vitamin D use in COVID-19 management.
Unveiling the Burden: A Six-Year Retrospective Analysis of Pressure Ulcer Epidemiology in a ICU
Authors: Vieira Sofia , Mostardinha António , Alves Paulo
Published: November 2024

Objective: This study describes the epidemiological changes in pressure ulcers (PUs) in a Portuguese intensive care unit (ICU) from January 2017 to June 2023, characterizes critically ill patients with PUs, identifies specific risk factors, and assesses the effectiveness of implemented preventive measures. Materials and Methods: A retrospective observational cohort study was conducted, analyzing records of ICU patients with PUs during the specified period. Data were extracted from the institution’s Global Risk Management application and the ICU’s electronic PU registry. The study included patients with ICU stays longer than 24 h and excluded those with ineligible clinical records or incomplete characterization data. Results: Among 3816 evaluated patients, 257 developed a total of 345 PUs, averaging 1.4 PUs per patient. The average PU prevalence rate was 6.81%, with the highest prevalence in 2020 (11.0%) and the lowest in 2022 (3.48%). The average incidence rate was 3.76%, peaking at 5.71% in 2020 and declining to 2.54% in 2023. The sacrum and heels were the most commonly affected areas, with Category 2 PUs being the most frequent. Key intrinsic risk factors included systemic diseases and sensory deficits, with pressure identified as a significant extrinsic factor. Preventive measures focused on risk assessment and pressure control. Conclusions: The study reveals a PU prevalence of 6.81% and an average incidence of 3.76%, underscoring the need for enhanced preventive strategies, especially in anatomical areas like the sacrum and heels. It emphasizes the importance of personalized assessments, continuous education for nursing staff, and a multidisciplinary approach to improve patient outcomes and care quality in the ICU.
Academic and Employment Preferences of Nursing Students at the University of Las Palmas of Gran Canaria: A Cross-Sectional Study
Authors: Ramos-Ramos Andrea , Rodríguez-Suárez Claudio Alberto , Díaz-González Candelaria de la Merced , Verdú-Soriano José , Berenguer-Pérez Miriam , González-de la Torre Héctor
Published: November 2024

Background/Objectives: It is well known that there are differences in the academic and employment preferences of nursing students once they have completed their undergraduate studies in nursing. These preferences are largely influenced by students’ affinity for certain thematic areas over others. Therefore, the objective of this study was to identify the employment and academic preferences of third- and fourth-year Nursing Degree students at the University of Las Palmas de Gran Canaria (ULPGC). Methods: A cross-sectional, descriptive observational study was conducted among nursing students across three campuses of the ULPGC (Canary Islands, Spain). An online questionnaire was used to collect various sociodemographic and academic variables, as well as preferences across ten thematic areas. Descriptive and bivariate inferential analyses were performed, along with a correlation analysis among the areas. Results: The areas of highest preference were “Emergency Nursing”, “General Nursing”, and “Family and Community Nursing”. The areas of lowest preference were “Other Areas (teaching, management, research)”, “Mental Health and Psychiatric Nursing”, and “Geriatric Nursing”. Three clusters of closely correlated areas were identified: cluster 1 (Intensive and Critical Care Nursing, Emergency and Emergency Nursing and Operating Theatre and Anaesthesia Nursing), cluster 2 (Obstetric-Gynaecological Nursing–Midwifery, Paediatric Nursing and Mental Health and Psychiatric Nursing) and cluster 3 (remaining areas). A significant proportion of students expressed intentions to pursue postgraduate studies. Conclusions: Strategies should be implemented to enhance students’ preferences in the areas of “Mental Health and Psychiatric Nursing” and “Geriatric Nursing”, which are areas where there is a high demand for nurses. It is also necessary to increase their interest in research, management, and teaching. This study was not registered.
The Impact of Obesity on Pain Perception During and After Subcutaneous Injections: A Cross-Sectional Analysis
Authors: Díaz-González Candelaria de la Merced , Ramal-López Josefa María , de la Rosa-Hormiga Milagros
Published: November 2024

(1) Background: The administration of subcutaneous (SC) injectables is among the most frequent procedures a nurse performs in daily practice. The needle for the injection must pass through the skin barrier to reach the SC space, where the drug will be deposited. This procedure can cause pain to the patient and local lesions. Local fat measurement can be performed by measuring the skin fold. Previous studies have found higher levels of pain in people with obesity receiving SC insulin, and this study thus aimed to measure pain levels during and after an SC injection of low-molecular-weight heparin (LMWH) and identify how age, gender, and obesity may modulate the level of pain during and after the procedure. (2) Methods: This was a cross-sectional study, and the variables included age, gender, body mass index (BMI), BMI quartile, abdominal skin folds (ASFs), ASF quartile, and pain level during and after injection. A caliper was used to measure ASFs, height and weight were used to calculate BMI, and the Visual Analog Scale (VAS) was used to measure pain. (3) Results: The sample amounted to 202 participants, which was not considered representative of the study population. The average age was 64.3 years, and females predominated (62.40%). Of these participants, 42.5% were obese, and 29.1% were overweight. The average pain levels were low during (1.4) and after injection (1.9), highlighting the absence of pain during injection in 29.7% and after injection in 34.2%. (4) Conclusions: Obesity was associated with increased pain, but when adjusted for age, the pain was no longer significant. Females and young participants showed a significant relationship with pain during injection. Age, gender, and obesity had a statistically significant relationship with pain level. Participants with obesity (according to BMI and ASF) showed the highest levels of pain during and after injection. After injection, there was an increase in pain in most cases, possibly due to the discomfort caused by the drug itself, an aspect considered in the drug’s technical data sheet as a frequent adverse effect (>1/10 to <1/100). However, the drug volume does not seem to be related to pain in this study.
Fasting in Judo—Between Healthy Weight Control and Health Hazard: A Narrative Review
Authors: Pocecco Elena , Schneider Friedemann , Stavrinou Pinelopi S. , De Crée Carl , Burtscher Johannes
Published: November 2024

Rapid weight loss strategies and weight cycling (losing and gaining weight repeatedly) are acknowledged problems in sports with weight categories, including judo. Extreme fasting, especially if combined with dehydration and intense physical exercise, may harm health and development, particularly those of children and teenagers engaging in judo competitions. However, there is substantial evidence for positive health effects of other forms of fasting. Here, we aim to provide an updated overview of the potential health risks associated with extreme dietary strategies in judo and contrast them with health-promoting fasting approaches. We conclude that the literature on the long-term health consequences of fasting in judo is scarce. This applies specifically to its potential association with eating disorders. Although many reports have highlighted the short-term risks of rapid weight loss, vulnerability and protection factors remain poorly understood. Rigorous scientific studies are needed to disentangle the factors that render fasting in judo healthy or unhealthy, considering both physical and mental health. We argue that a better understanding of weight management in judo is crucial to preventing health risks and designing healthy weight loss strategies, especially for young athletes.
Inventory of Shallow-Water Fouling Invertebrates of Long Island, New York
Authors: Roesch Ezra , Rosencrans Jack H. , Hatch Kent A. , Thacker Robert W.
Published: November 2024

Invasive marine invertebrates are increasingly recognized as a potential disturbance to coastal ecosystems. We sought to better document the taxonomic composition of subtidal communities around Long Island to obtain a baseline that can be used to monitor current and future invasions of non-indigenous species. We placed settlement blocks at 18 sites along the coast of Long Island, New York, for three months. After recovering blocks at 12 sites, we analyzed the taxonomic composition of fouling communities on the blocks. We observed 64 invertebrate and 3 algal taxa, with large variation in taxon richness among sites. Multivariate analyses revealed that although taxon composition was significantly dissimilar between north and south shores, variation in dissimilarity did not differ significantly between shores. The high variability in taxon composition observed among sites indicates that additional research is needed to expand our knowledge of invertebrate diversity in the waters surrounding Long Island. Adding more sites and replicate blocks within sites could improve future sampling designs. This research will benefit continuing efforts to monitor, manage, and prevent the establishment of marine invasive species.
Seagrass-Associated Biodiversity Influences Organic Carbon in a Temperate Meadow
Authors: O’Neill Lowri , Walter Bettina , Unsworth Richard K. F.
Published: November 2024

There is increasing interest in the role that seagrasses play in storing carbon in the context of climate mitigation, but many knowledge gaps in the factors controlling this storage exist. Here, we provide a small case study that examines the role of infaunal biodiversity in influencing seagrass and the carbon stored in its sediments. A total of 25 species of invertebrate were recorded in an intertidal Zostera marina meadow, where these species were dominated by polychaete worms with no bivalves present. We find organic carbon storage (within the top 20 cm) measured by AFDW to be highly variable within a small area of seagrass meadow ranging from 2961 gC.m−2 to 11,620 gC.m−2 with an average (±sd) of 64602 ± 3274 gC.m−2. Our analysis indicates that infaunal communities are significantly and negatively correlated with this sediment organic carbon. However, this effect is not as influential as hypothesised, and the relatively small sample size of the present study limits its ability to provide strong causality. Other factors, such as algal abundance, curiously had a potentially stronger influence on the carbon in the upper sediments. The increasing richness of infauna is likely reducing the build-up of organic carbon, reducing its ecosystem service role. We believe this to likely be the result of bioturbation by specific species such as Arenicola marina and Ampharete acutifrons. A change in sediment organic carbon suggests that these species could be key drivers of bioturbator-initiated redox-driven organic matter turnovers, influencing the microbial processes and remobilizing sediment compounds. Bioturbators should be considered as a limitation to Corg storage when managing seagrass Corg stocks; however, bioturbation is a natural process that can be moderated when an ecosystem is less influenced by anthropogenic change. The present study only provides small-scale correlative evidence with a range of surprising results; confirming these results within temperate seagrasses requires examining this process at large spatial scales or with targeted experiments.
How Does African Swine Fever Virus Evade the cGAS-STING Pathway?
Authors: Lin Can , Zhang Chenyang , Chen Nanhua , Meurens François , Zhu Jianzhong , Zheng Wanglong
Published: November 2024

African swine fever (ASF), a highly infectious and devastating disease affecting both domestic pigs and wild boars, is caused by the African swine fever virus (ASFV). ASF has resulted in rapid global spread of the disease, leading to significant economic losses within the swine industry. A significant obstacle to the creation of safe and effective ASF vaccines is the existing knowledge gap regarding the pathogenesis of ASFV and its mechanisms of immune evasion. The cyclic GMP–AMP synthase (cGAS)–stimulator of interferon genes (STING) pathway is a major pathway mediating type I interferon (IFN) antiviral immune response against infections by diverse classes of pathogens that contain DNA or generate DNA in their life cycles. To evade the host’s innate immune response, ASFV encodes many proteins that inhibit the production of type I IFN by antagonizing the cGAS-STING signaling pathway. Multiple proteins of ASFV are involved in promoting viral replication by protein–protein interaction during ASFV infection. The protein QP383R could impair the function of cGAS. The proteins EP364R, C129R and B175L could disturb the function of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP). The proteins E248R, L83L, MGF505-11L, MGF505-7R, H240R, CD2v, E184L, B175L and p17 could interfere with the function of STING. The proteins MGF360-11L, MGF505-7R, I215L, DP96R, A151R and S273R could affect the function of TANK Binding Kinase 1 (TBK1) and IκB kinase ε (IKKε). The proteins MGF360-14L, M1249L, E120R, S273R, D129L, E301R, DP96R, MGF505-7R and I226R could inhibit the function of Interferon Regulatory Factor 3 (IRF3). The proteins MGF360-12L, MGF505-7R/A528R, UBCv1 and A238L could inhibit the function of nuclear factor kappa B (NF-Κb).