bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison Link Function from Summer Rainfall Network in Amazon Basin
Authors: Sánchez P. C. Arturo , Calheiros Alan J. P. , Garcia Sâmia R. , Macau Elbert E. N.
Published: December 2023

The Amazon Basin is the largest rainforest in the world, and studying the rainfall in this region is crucial for understanding the functioning of the entire rainforest ecosystem and its role in regulating the regional and global climate. This work is part of the application of complex networks, which refer to a network modeled by graphs and are characterized by their high versatility, as well as the extraction of key information from the system under study. The main objective of this article is to examine the precipitation system in the Amazon basin during the austral summer. The networks are defined by nodes and connections, where each node represents a precipitation time series, while the connections can be represented by different similarity functions. For this study, three rainfall networks were created, which differ based on the correlation function used (Pearson, Spearman, and Kendall). By comparing these networks, we can identify the most effective method for analyzing the data and gain a better understanding of rainfall’s spatial structure, thereby enhancing our knowledge of its impact on different Amazon basin regions. The results reveal the presence of three important regions in the Amazon basin. Two areas were identified in the northeast and northwest, showing incursions of warm and humid winds from the oceans and favoring the occurrence of large mesoscale systems, such as squall lines. Additionally, the eastern part of the central Andes may indicate an outflow region from the basin with winds directed toward subtropical latitudes. The networks showed a high level of activity and participation in the center of the Amazon basin and east of the Andes. Regarding information transmission, the betweenness centrality identified the main pathways within a basin, and some of these are directly related to certain rivers, such as the Amazon, Purus, and Madeira. Indicating the relationship between rainfall and the presence of water bodies. Finally, it suggests that the Spearman and Kendall correlation produced the most promising results. Although they showed similar spatial patterns, the major difference was found in the identification of communities, this is due to the meridional differences in the network’s response. Overall, these findings highlight the importance of carefully selecting appropriate techniques and methods when analyzing complex networks.
HLA-DQ2/8 and COVID-19 in Celiac Disease: Boon or Bane
Authors: Lerner Aaron , Benzvi Carina , Vojdani Aristo
Published: December 2023

The SARS-CoV-2 pandemic continues to pose a global threat. While its virulence has subsided, it has persisted due to the continual emergence of new mutations. Although many high-risk conditions related to COVID-19 have been identified, the understanding of protective factors remains limited. Intriguingly, epidemiological evidence suggests a low incidence of COVID-19-infected CD patients. The present study explores whether their genetic background, namely, the associated HLA-DQs, offers protection against severe COVID-19 outcomes. We hypothesize that the HLA-DQ2/8 alleles may shield CD patients from SARS-CoV-2 and its subsequent effects, possibly due to memory CD4 T cells primed by previous exposure to human-associated common cold coronaviruses (CCC) and higher affinity to those allele’s groove. In this context, we examined potential cross-reactivity between SARS-CoV-2 epitopes and human-associated CCC and assessed the binding affinity (BA) of these epitopes to HLA-DQ2/8. Using computational methods, we analyzed sequence similarity between SARS-CoV-2 and four distinct CCC. Of 924 unique immunodominant 15-mer epitopes with at least 67% identity, 37 exhibited significant BA to HLA-DQ2/8, suggesting a protective effect. We present various mechanisms that might explain the protective role of HLA-DQ2/8 in COVID-19-afflicted CD patients. If substantiated, these insights could enhance our understanding of the gene–environment enigma and viral–host relationship, guiding potential therapeutic innovations against the ongoing SARS-CoV-2 pandemic.
Nutrient and Microbiome-Mediated Plant–Soil Feedback in Domesticated and Wild Andropogoneae: Implications for Agroecosystems
Authors: Quattrone Amanda , Yang Yuguo , Yadav Pooja , Weber Karrie A. , Russo Sabrina E.
Published: December 2023

Plants influence the abiotic and biotic environment of the rhizosphere, affecting plant performance through plant–soil feedback (PSF). We compared the strength of nutrient and microbe-mediated PSF and its implications for plant performance in domesticated and wild grasses with a fully crossed greenhouse PSF experiment using four inbred maize genotypes (Zea mays ssp. mays b58, B73-wt, B73-rth3, and HP301), teosinte (Z. mays ssp. parviglumis), and two wild prairie grasses (Andropogon gerardii and Tripsacum dactyloides) to condition soils for three feedback species (maize B73-wt, teosinte, Andropogon gerardii). We found evidence of negative PSF based on growth, phenotypic traits, and foliar nutrient concentrations for maize B73-wt, which grew slower in maize-conditioned soil than prairie grass-conditioned soil. In contrast, teosinte and A. gerardii showed few consistent feedback responses. Both rhizobiome and nutrient-mediated mechanisms were implicated in PSF. Based on 16S rRNA gene amplicon sequencing, the rhizosphere bacterial community composition differed significantly after conditioning by prairie grass and maize plants, and the final soil nutrients were significantly influenced by conditioning, more so than by the feedback plants. These results suggest PSF-mediated soil domestication in agricultural settings can develop quickly and reduce crop productivity mediated by PSF involving changes to both the soil rhizobiomes and nutrient availability.
Biodegradation of Cholesterol by Enterococcus faecium YY01
Authors: Yang Ruimin , Ahmad Shahbaz , Liu Hongyan , Xu Qianqian , Yin Chunhua , Liu Yang , Zhang Haiyang , Yan Hai
Published: December 2023

Cholesterol (CHOL) is one of the risk factors causing the blockage of the arterial wall, atherosclerosis, coronary heart disease, and other serious cardiovascular diseases. Here, a promising bacterial strain for biodegrading CHOL was successfully isolated from the gut of healthy individuals and identified as Enterococcus faecium YY01 with an analysis of the 16S rDNA sequence. An initial CHOL of 1.0 g/L was reduced to 0.5 g/L in 5 days, and glucose and beef extract were found to be optimal carbon and nitrogen sources for the rapid growth of YY01, respectively. To gain further insight into the mechanisms underlying CHOL biodegradation, the draft genome of YY01 was sequenced using Illumina HiSeq. Choloylglycine hydrolase, acyltransferase, and alkyl sulfatase was encoded by gene0586, gene1890, and gene2442, which play crucial roles in converting 3α, 7α, 12α-trihydroxy-5β-choranic acid to choline-CoA and then choline-CoA to bile acid. Notably, choloylglycine hydrolase was closely related to the biosynthesis of both primary and secondary bile acid. The findings of this study provide valuable insights into the metabolism pathway of CHOL biodegradation by YY01 and offer a potential avenue for the development of bacterioactive drugs against hypercholesterolemia.
Satureja hortensis L. and Calendula officinalis L., Two Romanian Plants, with In Vivo Antiparasitic Potential against Digestive Parasites of Swine
Authors: Băieş Mihai-Horia , Cotuţiu Vlad-Dan , Spînu Marina , Mathe Attila , Cozma-Petruț Anamaria , Bocǎneţ Vlad I. , Cozma Vasile
Published: December 2023

Internal parasitic diseases of swine constitute a major welfare and health concern in low-input livestock farming. Due to an increase in chemical resistance, phytotherapeutic remedies have become an alternative for the prophylaxis and therapy of digestive parasitosis, albeit few remedies have been subjected to scientific validation. Low-input swine farming in Romania has adopted the traditional use of phytotherapy for controlling pathogens in livestock. The current study aimed to assess the antiparasitic potential of Calendula officinalis and Satureja hortensis against digestive parasites of swine in two low-input farms. The fecal samples were collected from sows, fatteners, and weaners, and were tested using the following coproparasitological methods: centrifugal sedimentation, flotation (Willis, McMaster egg counting technique), Ziehl–Neelsen stain modified by Henricksen, modified Blagg method, and in vitro nematode larvae/protozoan oocyst cultures. Six species of digestive parasites were diagnosed, namely Ascaris suum, Trichuris suis, Oesophagostomum spp., Balantioides coli, Eimeria spp., and Cryptosporidium spp., in various combinations, dependent on the swine category. A dose of 140 mg/kg bw/day of C. officinalis and 100 mg/kg bw/day of S. hortensis powders administered for 10 consecutive days revealed a strong antiprotozoal and anthelmintic activity on the aforementioned parasites. The curative efficacy can be attributed to the presence of polyphenols, sterols, tocopherols, and methoxylated flavones. In conclusion, our results indicate that S. hortensis and C. officinalis are promising alternatives to the commercially available antiparasitics, enabling their use as natural antiparasitic products against gastrointestinal parasites in pigs.
Low-Valent-Tungsten-Catalyzed Aerobic Oxidative Cross-Dehydrogenative Coupling Reaction
Authors: Li Chunsheng , Chen Yaoyang , Ye Feihua , Chen Junhua , Zheng Jia
Published: December 2023

A straightforward and convenient protocol was established for the synthesis of thiophosphates and 3-sulfenylated indoles via low-valent-tungsten-catalyzed aerobic oxidative cross-dehydrogenative coupling reactions. These reactions occur under mild conditions and simple operations with commercially available starting materials, processing the advantage of excellent atom and step economy, broad substrate scope, and good functional groups tolerance. Moreover, this transformation could be practiced on the gram scale, which exhibits great potential in the preparation of drug-derived or bioactive molecules.
Further Improvement Based on Traditional Nanocapsule Preparation Methods: A Review
Authors: Zhou Yihong , Wang Peng , Wan Faling , Zhu Lifang , Wang Zongde , Fan Guorong , Wang Peng , Luo Hai , Liao Shengliang , Yang Yuling , Chen Shangxing , Zhang Ji
Published: December 2023

Nanocapsule preparation technology, as an emerging technology with great development prospects, has uniqueness and superiority in various industries. In this paper, the preparation technology of nanocapsules was systematically divided into three categories: physical methods, chemical methods, and physicochemical methods. The technological innovation of different methods in recent years was reviewed, and the mechanisms of nanocapsules prepared via emulsion polymerization, interface polymerization, layer-by-layer self-assembly technology, nanoprecipitation, supercritical fluid, and nano spray drying was summarized in detail. Different from previous reviews, the renewal iteration of core–shell structural materials was highlighted, and relevant illustrations of their representative and latest research results were reviewed. With the continuous progress of nanocapsule technology, especially the continuous development of new wall materials and catalysts, new preparation technology, and new production equipment, nanocapsule technology will be used more widely in medicine, food, cosmetics, pesticides, petroleum products, and many other fields.
Corrosion Behavior of Ni/NiCr/NiCrAlSi Composite Coating on Copper for Application as a Heat Exchanger in Sea Water
Authors: Du Hao , Wen Jiayuan , Song Guihong , Wu Hao , Yin Yansheng
Published: December 2023

This study introduces a novel Ni/NiCr/NiCrAlSi composite coating to enhance the corrosion resistance of copper, particularly for its use in marine heat exchangers. Utilizing characterization techniques such as scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), potentiodynamic polarization, and electrochemical impedance spectroscopy (EIS), the paper investigates the coating’s composition, structure, and corrosion resistance in 3.5 wt.% NaCl aqueous solutions. A significant focus is placed on the role of aluminum within the NiCrAlSi layer, examining its influence on the coating’s structure and corrosion behavior. The results indicate that the NiCrAlSi layer with an aluminum content of 5.49 at.% exhibits the most improved corrosion resistance, characterized by the highest corrosion potential and a corrosion current density that is more than one order of magnitude lower compared to the Ni/NiCr coating. The effectiveness of this composite coating is attributed to its multilayer structure and the synergistic effect of alloying elements Cr, Al, and Si, which collectively inhibit corrosive medium penetration. These insights present the Ni/NiCr/NiCrAlSi coating as a promising candidate for copper protection in sea water environments, merging enhanced durability with cost-effectiveness.
Microstructural Characterization and Magnetic, Dielectric, and Transport Properties of Hydrothermal La2FeCrO6 Double Perovskites
Authors: Yi Kang , Wu Zhiwei , Tang Qingkai , Gu Jiayuan , Ding Jie , Chen Liangdong , Zhu Xinhua
Published: December 2023

Double perovskite La2FeCrO6 (LFCO) powders were synthesized via the hydrothermal method, which crystallized in an orthorhombic (Pnma) structure and exhibited a spherical morphology with an average particle size of 900 nm. Fourier transform infrared spectroscopy demonstrated the presence of fingerprints of vibrational modes of [FeO6] and [CrO6] octahedra in the powders. The XPS spectra revealed dual oxide states of Fe (Fe2+/Fe3+) and Cr (Cr3+/Cr4+) elements, and the oxygen element appeared as lattice oxygen and defect oxygen, respectively. The LFCO powders exhibited weak ferromagnetic behavior at 5 K with a Curie temperature of 200 K. Their saturation magnetization and coercive field were measured as 0.31 μB/f.u. and 8.0 kOe, respectively. The Griffiths phase was observed between 200 K and 223 K. A butterfly-like magnetoresistance (MR)–magnetic field (H) curve was observed in the LFCO ceramics at 5 K with an MR (5 K, 6 T) value of −4.07%. The temperature dependence of resistivity of the LFCO ceramics demonstrated their semiconducting nature. Electrical transport data were fitted by different conduction models. The dielectric behaviors of the LFCO ceramics exhibited a strong frequency dispersion, and a dielectric abnormality was observed around 260 K. That was ascribed to the jumping of electrons trapped at shallow levels created by oxygen vacancies. The dielectric loss showed relaxation behavior between 160 K and 260 K, which was attributed to the singly ionized oxygen vacancies.
Dietary Intake Is Not Associated with Body Composition nor with Biochemical Tests but with Psychological Status of Cancer Patients Receiving Chemotherapy
Authors: Subih Hadil S. , Al-Shwaiyat Esraa A. , Al-Bayyari Nahla , Obeidat Belal S. , Abu-Farsakh Fadi , Bawadi Hiba
Published: December 2023

Chemotherapy can negatively affect cancer patients’ eating patterns, psychological status, body composition, and quality of life. In this study, we aimed to investigate the correlations between dietary intake/care and the psychological status of cancer patients treated with chemotherapy. An observational study was conducted on 75 participants during their first cycle of chemotherapy treatment, and they were followed up for three cycles. Each participant completed a reliable validated questionnaire, psychological questionnaire, quality of life questionnaire, and three-day food records. Dietary intake was considered adequate if there was an adherence of participants to dietary recommendations offered by the dietitian and was confirmed by ESHA analysis software (version 10.6.3). Seventy-five percent of participants had inadequate nutrition intake. All anthropometric measurements decreased after 2 months of chemotherapy regardless of patients’ dietary intake. Approximately half of the participants reported depression and anxiety. There were significant differences between all nutrient intake levels when compared to the recommended dietary allowance except for fat, unsaturated fatty acids, and iron. Also, there were associations between nutritional intake and life quality and depression. In conclusion, poor dietary intake was associated with depression and insufficient macro- and micronutrient intake. Emotional and nutritional support from healthcare providers and family are highly necessary.
Dietary Inflammatory Potential in Pediatric Diseases: A Narrative Review
Authors: Tosi Martina , Montanari Chiara , Bona Federica , Tricella Chiara , Agostinelli Marta , Dolor Jonabell , Chillemi Claudia , Di Profio Elisabetta , Tagi Veronica Maria , Vizzuso Sara , Fiore Giulia , Zuccotti Gianvincenzo , Verduci Elvira
Published: December 2023

Inflammatory status is one of the main drivers in the development of non-communicable diseases (NCDs). Specific unhealthy dietary patterns and the growing consumption of ultra-processed foods (UPFs) may influence the inflammation process, which negatively modulates the gut microbiota and increases the risk of NCDs. Moreover, several chronic health conditions require special long-term dietary treatment, characterized by altered ratios of the intake of nutrients or by the consumption of disease-specific foods. In this narrative review, we aimed to collect the latest evidence on the pro-inflammatory potential of dietary patterns, foods, and nutrients in children affected by multifactorial diseases but also on the dietetic approaches used as treatment for specific diseases. Considering multifactorial diet-related diseases, the triggering effect of pro-inflammatory diets has been addressed for metabolic syndrome and inflammatory bowel diseases, and the latter for adults only. Future research is required on multiple sclerosis, type 1 diabetes, and pediatric cancer, in which the role of inflammation is emerging. For diseases requiring special diets, the role of single or multiple foods, possibly associated with inflammation, was assessed, but more studies are needed. The evidence collected highlighted the need for health professionals to consider the entire dietary pattern, providing balanced and healthy diets not only to permit the metabolic control of the disease itself, but also to prevent the development of NCDs in adolescence and adulthood. Personalized nutritional approaches, in close collaboration between the hospital, country, and families, must always be promoted together with the development of new methods for the assessment of pro-inflammatory dietary habits in pediatric age and the implementation of telemedicine.
Development of Interventions to Support Provincial Implementation of the Baby-Friendly Initiative: A Study Protocol
Authors: Benoit Britney , Cassidy Christine , Campbell-Yeo Marsha , Gillis Doris , Kirk Sara , Sim S. Meaghan , LeDrew Michelle , Loring Sally , Tomblin Murphy Gail , Elliott Rose Annette , Betker Claire , MacKeen Leanne , Arseneau Lindsay , Shebib Kim , Reid Trudy , Daman Ripu
Published: December 2023

Breastfeeding is internationally recognized as the optimal form of infant nutrition. The Baby-Friendly Initiative (BFI) is an evidence-informed program that leads to improved breastfeeding outcomes. Despite the benefits of breastfeeding, Nova Scotia has one of the lowest breastfeeding rates in Canada. Additionally, only two birthing hospitals in the province have BFI designation. We aim to address this gap using a sequential qualitative descriptive design across three phases. In Phase 1, we will identify barriers and facilitators to BFI implementation through individual, semi-structured interviews with 40 health care professionals and 20 parents. An analysis of relevant policy and practice documents will complement these data. In Phase 2, we will develop implementation interventions aimed at addressing the barriers and facilitators identified in Phase 1. An advisory committee of 10–12 administrative, clinical, and parent partners will review these interventions. In Phase 3, the interventions will be reviewed by a panel of 10 experts in BFI implementation through an online survey. Feedback on the revised implementation interventions will then be sought from 20 health system and parent partners through interviews. This work will use implementation science methods to support integrated and sustained implementation of the BFI across hospital/community and rural/urban settings in Nova Scotia. This study was not registered.
Recent Developments in Lateral Flow Assays for Salmonella Detection in Food Products: A Review
Authors: Silva Gabrielle B. L. , Campos Fabiana V. , Guimarães Marco C. C. , Oliveira Jairo P.
Published: December 2023

Salmonellosis is a disease transmitted by contaminated food and is one of the leading causes of infections worldwide, making the early detection of Salmonella of crucial importance for public health. However, current detection methods are laborious and time-consuming, thus impacting the entire food supply chain and leading to production losses and economic sanctions. To mitigate these issues, a number of different biosensors have been developed, including lateral flow assays (LFAs), which have emerged as valuable tools in pathogen detection due to their portability, ease of use, time efficiency, and cost effectiveness. The performance of LFAs has been considerably enhanced by the development of new nanomaterials over the years. In this review, we address the principles and formats of the assay and discuss future prospects and challenges with an emphasis on LFAs developed for the detection of different Salmonella serovars in food.
Analysis of Sporulation in Bacillus cereus Biovar anthracis Which Contains an Insertion in the Gene for the Sporulation Factor σK
Authors: Gummelt Constanze , Dupke Susann , Howaldt Sabine , Zimmermann Fee , Scholz Holger C. , Laue Michael , Klee Silke R.
Published: December 2023

Bacillus cereus biovar anthracis (Bcbva) is an untypical pathogen causing a fatal anthrax-like disease in a variety of wildlife species in African rainforest areas. In contrast to Bacillus anthracis and most species of the B. cereus group, all strains of the Bcbva cluster contain a 22 kb insertion in the sigK gene which encodes the essential late sporulation sigma factor σK. This insertion is excised during sporulation in a site-specific recombination process resulting in an intact sigK gene and a circular molecule. The sporulation kinetics of two strains each of Bcbva and B. anthracis were compared by the expression analysis of eight sporulation-associated genes, including sigK, using reverse transcriptase quantitative real-time PCR. In addition, morphological sporulation stages were analyzed and quantified by electron microscopy. Our results indicated that the necessary excision of the insertion in Bcbva neither delayed nor inhibited its sporulation. In two spontaneous mutants of Bcbva, the excision of the sigK insertion and sporulation were impeded due to mutations in the spo0A and spoVG regulator genes, respectively. The spo0A frameshift mutation was overcome by intragenic suppression in a revertant which was able to sporulate normally, despite an M171S amino acid exchange in the global regulator Spo0A. A screening of the NCBI database identified further strains of the B. cereus group which possess unrelated insertions in the sigK gene, and two strains containing almost identical insertions at the same gene position. Some of the sigK insertions encode putative prophages, whereas the Bcbva insertion encoded a type I restriction–modification system. The function of these insertions and if they are possibly essential for sporulation remains to be assessed.
Plasmodium vivax Malaria and G6PD Testing
Authors: Ley Benedikt , Luzzatto Lucio
Published: December 2023

Early malaria investigators were certainly correct in classifying the species falciparum and the species vivax as belonging to the same genus, Plasmodium [...]
Glibenclamide-Loaded Engineered Nanovectors (GNVs) Modulate Autophagy and NLRP3-Inflammasome Activation
Authors: Saresella Marina , Zoia Chiara Paola , La Rosa Francesca , Bazzini Chiara , Sala Gessica , Grassenis Erica , Marventano Ivana , Hernis Ambra , Piancone Federica , Conti Elisa , Sesana Silvia , Re Francesca , Seneci Pierfausto , Ferrarese Carlo , Clerici Mario
Published: December 2023

Activation of the NLRP3 inflammasome in response to either exogenous (PAMPs) or endogenous (DAMPs) stimuli results in the production of IL-18, caspase-1 and IL-1β. These cytokines have a beneficial role in promoting inflammation, but an excessive activation of the inflammasome and the consequent constitutive inflammatory status plays a role in human pathologies, including Alzheimer’s disease (AD). Autophagic removal of NLRP3 inflammasome activators can reduce inflammasome activation and inflammation. Likewise, inflammasome signaling pathways regulate autophagy, allowing the development of inflammatory responses but preventing excessive and detrimental inflammation. Nanotechnology led to the development of liposome engineered nanovectors (NVs) that can load and carry drugs. We verified in an in vitro model of AD-associated inflammation the ability of Glibenclamide-loaded NVs (GNVs) to modulate the balance between inflammasome activation and autophagy. Human THP1dM cells were LPS-primed and oligomeric Aß-stimulated in the presence/absence of GNVs. IL-1β, IL-18 and activated caspase-1 production was evaluated by the Automated Immunoassay System (ELLA); ASC speck formation (a marker of NLRP3 activation) was analyzed by FlowSight Imaging flow-cytometer (AMNIS); the expression of autophagy targets was investigated by RT-PCR and Western blot (WB); and the modulation of autophagy-related up-stream signaling pathways and Tau phosphorylation were WB-quantified. Results showed that GNVs reduce activation of the NLRP3 inflammasome and prevent the Aß-induced phosphorylation of ERK, AKT, and p70S6 kinases, potentiating autophagic flux and counteracting Tau phosphorylation. These preliminary results support the investigation of GNVs as a possible novel strategy in disease and rehabilitation to reduce inflammasome-associated inflammation.
Antiviral Activity and Molecular Dynamics Simulation of Hops Compounds against Oropouche Virus (Peribunyaviridae)
Authors: Mandova Tsvetelina , Saivish Marielena Vogel , Menezes Gabriela de Lima , Bezerra Katyanna Sales , Fulco Umberto Laino , da Silva Roosevelt Alves , Da Costa Fernando Batista , Nogueira Maurício Lacerda
Published: December 2023

The Oropouche virus (OROV) is a member of the family Peribunyaviridae (order Bunyavirales) and the cause of a dengue-like febrile illness transmitted mainly by biting midges and mosquitoes. In this study, we aimed to explore acylphloroglucinols and xanthohumol from hops (Humulus lupulus L.) as a promising alternative for antiviral therapies. The evaluation of the inhibitory potential of hops compounds on the viral cycle of OROV was performed through two complementary approaches. The first approach applies cell-based assay post-inoculation experiments to explore the inhibitory potential on the latest steps of the viral cycle, such as genome translation, replication, virion assembly, and virion release from the cells. The second part covers in silico methods evaluating the ability of those compounds to inhibit the activity of the endonuclease domain, which is essential for transcription, binding, and cleaving RNA. In conclusion, the beta acids showed strongest inhibitory potential in post-treatment assay (EC50 = 26.7 µg/mL). Xanthohumol had the highest affinity for OROV endonuclease followed by colupulone and cohumulone. This result contrasts with that observed for docking and MM/PBSA analysis, where cohumulone was found to have a higher affinity. Finally, among the three tested ligands, Lys92 and Arg33 exhibited the highest affinity with the protein.
Extracellular Matrices as Bioactive Materials for In Situ Tissue Regeneration
Authors: Zhao Peng , Yang Fengbo , Jia Xiaoli , Xiao Yuqin , Hua Chao , Xing Malcolm , Lyu Guozhong
Published: December 2023

Bioactive materials based on a nature-derived extracellular matrix (NECM) represent a category of biomedical devices with versatile therapeutic applications in the realms of tissue repair and engineering. With advancements in decellularization technique, the inherent bioactive molecules and the innate nano-structural and mechanical properties are preserved in three-dimensional scaffolds mainly composed of collagens. Techniques such as electrospinning, three-dimensional printing, and the intricate fabrication of hydrogels are developed to mimic the physical structures, biosignalling and mechanical cues of ECM. Until now, there has been no approach that can fully account for the multifaceted properties and diverse applications of NECM. In this review, we introduce the main proteins composing NECMs and explicate the importance of them when used as therapeutic devices in tissue repair. Nano-structural features of NECM and their applications regarding tissue repair are summarized. The origins, degradability, and mechanical property of and immune responses to NECM are also introduced. Furthermore, we review their applications, and clinical features thereof, in the repair of acute and chronic wounds, abdominal hernia, breast deformity, etc. Some typical marketed devices based on NECM, their indications, and clinical relevance are summarized.
Influence of the Spectral Composition of Illuminating Light Sources on Biometric and Phytochemical Characteristics of Ocimum basilicum L.
Authors: Degtereva Mariya , Levin Yevgeniy , Gubina Anastasia , Degterev Aleksandr , Lamkin Ivan , Konoplev Georgii , Tarasov Sergey , Whaley Andrei , Whaley Anastasiia , Suloev Ivan , Danilova Alexandra , Gusev Konstantin , Maimistov Denis
Published: December 2023

Precise adaptation of the greenhouse lighting spectrum to basic photophysiological processes can effectively and directionally stimulate plant growth and development. The optimal spectrum depends on the plant species and the stage of development and could be assessed empirically. The aim of this study is to determine the LED illumination spectrum that provides a significant improvement in the growth rate and accumulation of biologically active compounds for basil plants (Ocimum basilicum L.) under hydroponic cultivation compared to more traditional lighting sources. The following light sources with various emission spectra were used: an LED lamp within a spectral range of 400–800 nm (B:G:R 15%:5%:80%); a high-pressure sodium lamp (HPS) (B:G:R 5%:45%:50%); a compact fluorescent lamp (B:G:R 20%:40%:40%); a grow LED strip (B:G:R 15%:40%:45%); a white LED lamp (B:G:R 30%:45%:25%); a customized LED lighting setup in color ratios 100%B, 75%B + 25%R, 50%B + 50%R, 25%B + 75%R, 100%R, and natural lighting. A photosynthetic photon flux density (PPFD) of 150 μmol∙m−2∙s−1 was provided with all the sources. It was demonstrated reliably that employing the LED strip as an illumination device gives a 112% increase in basil plant yield compared to the HPS; the transpiration coefficient for the LED strip is six times lower than for the HPS. The content of flavonoids in the basil aerial parts on the 30th, 50th, and 70th days of development is 3.2 times higher than for the HPS; the metabolite composition is also more uniform for LED strip lighting.
Mode-Independent Optical Switch Based on Graphene-Polymer Hybrid Waveguides
Authors: Lian Tianhang , Xie Yuhang , Yu Qidong , Sun Shijie , Sun Xiaoqiang , Wang Xibin , Zhang Daming
Published: December 2023

Mode-division multiplexing (MDM) is a promising multiplexing technique to further improve the transmission capacity of optical communication and on-chip optical interconnection systems. Furthermore, the multimode optical switch is of great importance in the MDM system, since it makes the MDM system more flexible by directly switching multiple spatial signals simultaneously. In this paper, we proposed a mode-independent optical switch based on the graphene–polymer hybrid waveguide platform that could process the TE11, TE12, TE21 and TE22 modes in a few-mode waveguide. The presented switch is independent of the four guided modes, optimizing the buried position of graphene capacitors in the polymer waveguide to regulate the coplanar interaction between the graphene capacitors and spatial modes. The TE11, TE12, TE21 and TE22 modes can be regulated simultaneously by changing the chemical potential of graphene capacitors in a straight waveguide. Our presented switch can enable the independent management of the spatial modes to be more flexible and efficient and has wide application in the MDM transmission systems.
Sweet Basil (Ocimum basilicum L.)―A Review of Its Botany, Phytochemistry, Pharmacological Activities, and Biotechnological Development
Authors: Azizah Nabilah Sekar , Irawan Budi , Kusmoro Joko , Safriansyah Wahyu , Farabi Kindi , Oktavia Dina , Doni Febri , Miranti Mia
Published: December 2023

An urgent demand for natural compound alternatives to conventional medications has arisen due to global health challenges, such as drug resistance and the adverse effects associated with synthetic drugs. Plant extracts are considered an alternative due to their favorable safety profiles and potential for reducing side effects. Sweet basil (Ocimum basilicum L.) is a valuable plant resource and a potential candidate for the development of pharmaceutical medications. A single pure compound or a combination of compounds exhibits exceptional medicinal properties, including antiviral activity against both DNA and RNA viruses, antibacterial effects against both Gram-positive and Gram-negative bacteria, antifungal properties, antioxidant activity, antidiabetic potential, neuroprotective qualities, and anticancer properties. The plant contains various phytochemical constituents, which mostly consist of linalool, eucalyptol, estragole, and eugenol. For centuries, community and traditional healers across the globe have employed O. basilicum L. to treat a wide range of ailments, including flu, fever, colds, as well as issues pertaining to digestion, reproduction, and respiration. In addition, the current research presented underscores the significant potential of O. basilicum-related nanotechnology applications in addressing diverse challenges and advancing numerous fields. This promising avenue of exploration holds great potential for future scientific and technological advancements, promising improved utilization of medicinal products derived from O. basilicum L.
Plant Nutrition—New Methods Based on the Lessons of History: A Review
Authors: Kulhánek Martin , Asrade Dinkayehu Alamnie , Suran Pavel , Sedlář Ondřej , Černý Jindřich , Balík Jiří
Published: December 2023

As with new technologies, plant nutrition has taken a big step forward in the last two decades. The main objective of this review is to briefly summarise the main pathways in modern plant nutrition and attract potential researchers and publishers to this area. First, this review highlights the importance of long-term field experiments, which provide us with valuable information about the effects of different applied strategies. The second part is dedicated to the new analytical technologies (tomography, spectrometry, and chromatography), intensively studied environments (rhizosphere, soil microbial communities, and enzymatic activity), nutrient relationship indexes, and the general importance of proper data evaluation. The third section is dedicated to the strategies of plant nutrition, i.e., (i) plant breeding, (ii) precision farming, (iii) fertiliser placement, (iv) biostimulants, (v) waste materials as a source of nutrients, and (vi) nanotechnologies. Finally, the increasing environmental risks related to plant nutrition, including biotic and abiotic stress, mainly the threat of soil salinity, are mentioned. In the 21st century, fertiliser application trends should be shifted to local application, precise farming, and nanotechnology; amended with ecofriendly organic fertilisers to ensure sustainable agricultural practices; and supported by new, highly effective crop varieties. To optimise agriculture, only the combination of the mentioned modern strategies supported by a proper analysis based on long-term observations seems to be a suitable pathway.
Are Adult Mosquito Control Products (Adulticides) Harmful? A Review of the Potential Human Health Impacts from Exposure to Naled and Dichlorvos (DDVP)
Authors: Mendoza Daniel L. , Peterson Robert K. D. , Bonds Jane A. S. , White Gregory S. , Faraji Ary
Published: December 2023

We performed a thorough systematic review of published literature to determine potential links between human health impacts and naled, a registered adult mosquito control product (adulticide), and its major degradate, dichlorvos (DDVP). A search query was performed on 8 September 2023, capturing all articles published up to that date on the Scopus and PubMed databases. Inclusion criteria were the presence of either pesticide and a measured or modeled human health outcome or risk. The search string resulted in 382 articles; however, 354 articles were excluded, resulting in only 28 articles that met the inclusion criteria. The studies that directly relate to aerosolized ultra-low volume (ULV) mosquito control applications did not report any associated deleterious human health outcomes. Results from the reviewed papers displayed no negative health effects or led to inconclusive results. No studies showed adverse health effects from aerial ULV applications for mosquito management. Our findings are congruent with the United States Environmental Protection Agency and Centers for Disease Control and Prevention recommendations that aerial applications of naled, following label parameters, do not pose an adverse risk exposure to humans, wildlife, and the environment.
What Are “Bioplastics”? Defining Renewability, Biosynthesis, Biodegradability, and Biocompatibility
Authors: Lackner Maximilian , Mukherjee Anindya , Koller Martin
Published: December 2023

Today, plastic materials are mostly made from fossil resources, and they are characterized by their long lifetime and pronounced persistence in the open environment. These attributes of plastics are one cause of the ubiquitous pollution we see in our environment. When plastics end up in the environment, most of this pollution can be attributed to a lack of infrastructure for appropriately collecting and recycling plastic waste, mainly due to mismanagement. Because of the huge production volumes of plastics, their merits of being cheap to produce and process and their recalcitrance have turned into a huge disadvantage, since plastic waste has become the end point of our linear economic usage model, and massive amounts have started to accumulate in the environment, leading to microplastics pollution and other detrimental effects. A possible solution to this is offered by “bioplastics”, which are materials that are either (partly) biobased and/or degradable under defined conditions. With the rise of bioplastics in the marketplace, several standards and test protocols have been developed to assess, certify, and advertise their properties in this respect. This article summarizes and critically discusses different views on bioplastics, mainly related to the properties of biodegradability and biobased carbon content; this shall allow us to find a common ground for clearly addressing and categorizing bioplastic materials, which could become an essential building block in a circular economy. Today, bioplastics account for only 1–2% of all plastics, while technically, they could replace up to 90% of all fossil-based plastics, particularly in short-lived goods and packaging, the single most important area of use for conventional plastics. Their replacement potential not only applies to thermoplastics but also to thermosets and elastomers. Bioplastics can be recycled through different means, and they can be made from renewable sources, with (bio)degradability being an option for the mismanaged fraction and special applications with an intended end of life in nature (such as in seed coatings and bite protection for trees). Bioplastics can be used in composites and differ in their properties, similarly to conventional plastics. Clear definitions for “biobased” and “biodegradable” are needed to allow stakeholders of (bio)plastics to make fact-based decisions regarding material selection, application, and end-of-life options; the same level of clarity is needed for terms like “renewable carbon” and “bio-attributed” carbon, definitions of which are summarized and discussed in this paper.
Development of an In Situ Photo-Crosslinking Antimicrobial Collagen Hydrogel for the Treatment of Infected Wounds
Authors: Wu Song-Yi , Tsai Wei-Bor
Published: December 2023

Antimicrobial hydrogels have received considerable attention in the treatment of bacteria-infected wounds. Herein, we develop a neutral, soluble collagen via modification with maleic anhydride, serving as a hydrogel precursor. Maleic anhydride-modified collagen (ColME) could form a gel after exposure to UV light and be loaded with the antimicrobial agents, nisin and levofloxacin, to acquire antimicrobial ability. The ColME hydrogel containing nisin and levofloxacin had good cytocompatibility and effectively killed pathogenic bacterial strains, such as Escherichia coli and Staphylococcus aureus. The antimicrobial ColME hydrogels effectively supported the healing of a full-thickness skin wound infected with S. aureus in a mouse model. Our results demonstrate the potential of antimicrobial hydrogels as effective wound dressings via in situ photogelation for the healing of infected wounds.
Improving the Physical Properties of Starch-Based Powders for Potential Anti-Adhesion Applications
Authors: Sun Jaydon , Fang Tzu-Shan , Chen Yu-Xiang , Tsai Yu-Cheng , Liu Yi-Xin , Chen Chih-Yu , Su Chen-Ying , Fang Hsu-Wei
Published: December 2023

Postoperative adhesion is one of the most common complications that occur during and after surgery; thus, materials that can prevent adhesion are often applied. Starch powders with a high water absorption capacity are preferred, and many studies have focused on increasing the water absorption of modified starches, as native starch powders display poor water-holding capacities. The effects of salts on the physical properties of acetylated distarch phosphate potato starch powders were investigated here. Changes in functional groups, the crystal structures of modified starch, particle morphologies, water absorption, viscosity, and in vivo adhesion were investigated. The results showed that salts greatly improved the water absorption and viscosity of acetylated distarch phosphate potato starch powders. Among the three different salt-modified starch powders, NaCl-modified starch powders displayed higher water absorption and viscosity and demonstrated better in vivo anti-adhesion performance. The results of this study propose a potential biomaterial that may function as an anti-adhesive, potentially leading to reduced surgical risks and a better quality of life for patients.
Dimensionless Number Group Analysis of Surface-Treated Powders
Authors: Ludwig Bellamarie
Published: December 2023

Modeling powder properties remains a complex and difficult area of study because particulate materials can behave differently under variable conditions based on their bulk and surface-level properties. The research presented in this manuscript was designed to support the fundamental understanding of powder systems by joining experimental and theoretical calculations of dimensionless numbers groups for design purposes. In order to do so, this work focused on two critical variables to better understand fluidization design: physical and chemical surface properties. To better resolve the influence of surface properties, surface-treated powders were used. Five different powder samples of varying particle size distribution were characterized using physical property measurements, including pressure drop profiles to obtain the minimum fluidization velocity, density measurements, and particle sizing. Using theoretical equations, the minimum fluidization velocity was also calculated to compare with those obtained experimentally and determine typical dimensionless number groups used in bulk handling system design. The results showed that the theoretically determined values were lower than those calculated using the experimentally umf. In the case of the Reynolds number, the experimental values were 3–20% higher than the theoretical values, which is an important distinction for designing conveying systems and pipeline flow. Similar results were observed for the theoretical and experimental Froude numbers, indicating an important dependence on the cohesive properties of the particle interactions. Additional dimensionless number groups were considered, including the granular bond number and flow factors. To investigate the influence of surface forces, Hamaker constants were utilized for alumina and polydimethylsiloxane in the calculation of the granular bond number. A lower granular bond was observed with a decrease in the Hamaker constant for PDMS, suggesting that the surface forces would be lower for our surface-treated powders.
Rigid Polyurethane Foams Modified with Soybean-Husk-Derived Ash as Potential Insulating Materials
Authors: Magiera Anna , Kuźnia Monika , Błoniarz Aleksandra , Magdziarz Aneta
Published: December 2023

One of the most popular polymeric materials in the building and construction industry is rigid polyurethane foam (RPUF). In order to reduce the number of expensive chemical components, various fillers are used in the RPUF industry. The aim of this work was to investigate the influence of the biomass originated filler soybean-husk-derived ash on the structure and properties of composite RPUF. Firstly, polyurethane foams were obtained using hand mixing and casting techniques. Composite foams contained 5, 10, 15, and 20 wt. % of the filler. Secondly, the obtained composite materials were analyzed considering their cellular structure using optical microscopy and image processing software. All samples were composed of mostly pentagonal, regular-in-shape cells. Their diameters ranged between 100 and 70 µm. The chemical structure of the foams was investigated using infrared spectroscopy. No chemical interactions between matrix and filler were detected. Mechanical testing was performed in order to evaluate the mechanical performance of the materials. Both compressive strength and Young’s modulus were comparable and equaled approx. 130 kPa and 2.5 MPa, respectively. Wettability analysis indicated a hydrophobic nature of the materials. The obtained results suggested that the cellular and chemical structure of the polyurethane matrix was not affected by the filler incorporation.