bluuebunny/crossref_metadata_2025 huggingface dataset:
Dermis resident macrophages orchestrate
localized ILC2-eosinophil circuitries to maintain their M2-like properties and promote
non-healing cutaneous leishmaniasis
Authors: Lee Sang Hun , Kang Byunghyun , Kamenyeva Olena , Ferreira Tiago , Cho Kyoungin , Khillan Jaspal , Kabat Juraj , Kelsall Brian , Sacks David
Published: April 2023

Tissue-resident macrophages (TRMs) are critical for tissue homeostasis/repair. We previously showed that dermal TRMs produce CCL24 (eotaxin2) which mediates their interaction with IL-4 producing eosinophils, required to maintain their number and M2-like properties in the TH1 environment of the Leishmania major infected skin. Here, we unveil another layer of TRM self-maintenance involving their production of TSLP, an alarmin typically characterized as epithelial cell-derived. Both TSLP signaling and IL-5+ innate lymphoid cell 2 (ILC2s) were shown to maintain the number of dermal TRMs and promote infection. Single cell RNA sequencing identified the dermal TRMs as the sole source of TSLP and CCL24. Development of Ccl24-cre mice permitted specific labeling of dermal TRMs, as well as interstitial TRMs from other organs. Genetic ablation of TSLP from dermal TRMs reduced the number of dermal TRMs, and disease was ameliorated. Thus, by orchestrating localized type 2 circuitries with ILC2s and eosinophils, dermal TRMs are self-maintained as a replicative niche for L. major.
Artificial intelligence system for evaluating county-level government social governance based on heterogeneous network
Authors: Ma Yutong , Xie Bingxue
Published: April 2023

With the rapid development of mobile communication network technology and the increase of various demands, users have put forward higher requirements for the performance of today's communication technology. Different types of communication subnets can be combined to create different heterogeneous network technologies, which can effectively expand network coverage and improve system communication performance. This paper studies the allocation and decomposition of D2D resources into different heterogeneous network systems. In contrast, this article optimizes the functions of the social information system based on management needs, and integrates the artificial intelligence system into a multi-case study method set of three components based on the correlation between the advantages of the equal embedded artificial intelligence system, and integrates the social management the most common situations in information systems are the subject of research. Research shows that the three basic elements of artificial intelligence systems have been incorporated into social management information systems. The ability, perception, and political promotion of the artificial intelligence system were unearthed, thereby establishing a county-level government social governance evaluation system. Reasonably determining the weights of indicators at all levels is the key to a reasonable assessment of social governance. The weight values ​​of indicators at all levels directly affect the results of the comprehensive evaluation of social governance by county-level governments. The indicator system should use expert scoring methods to determine the weight of each indicator. In terms of data collection, it can be collected through annual statistical data and questionnaire data. Finally, a comprehensive social governance index will be formed for county-level governments, and further research and analysis will be required in terms of index analysis, weighting, and data availability.
Application of fuzzy image processing and Iot real-time monitoring in sports training mode
Authors: Shi Longlong , Liu Siwen , Zhang Ligong , Zhu Yongguo
Published: April 2023

Blurred images are very complicated because imaging is affected by computer technology, sensor technology, vision, mathematics, environment, human activities, etc. Sometimes the available visual information is not what we can accept. The image sometimes has poor contrast, and due to different levels of damage, the sound or blind spots are also different. The Internet of Things has become the main focus of the industry. It can be considered as an extension of the three existing networks of Internet, telecommunications, and radio and television networks. And it gradually surpassed the interaction of people's information, and expanded to the information exchange between people and things, things and people, things and things. With the rapid development of the Internet of Things technology, especially the diversity of remote transmission methods and the improvement of the technical level, the remote real-time monitoring of the Internet of Things has been more and more widely used in the Internet. In the IoT monitoring system, the service is the bridge between data collection and data application. In order to improve the efficiency of the communication module and support the availability of multiple devices, it is very important to design an efficient communication server. Through real-time analysis of the exercise data brought by multiple students, personalized exercise training plans can be customized according to the different physiques of the students. It can be seen from the results of the simulation experiment that whether it is a separate analysis or an objective judgment, the test results show that the method in this paper is effective and it is possible to improve the image quality.
Superconductivity in Twisted Bilayer Graphene: Cooper-pairs distribution function analysis
Authors: González-Pedreros Guillermo , Camargo-Martínez Jose , Mesa Fredy
Published: April 2023

Cooper-pair distribution function Dcp(ω, Tc) for Twisted Bilayer Graphene (TBLG) in the presence of an external electric field is presented. The TBLG structure is proposed in the geometric approximation frame that allows mimicking structures with layers rotated in tiny angles, avoiding supercell calculations. The results show that the proposed geometric method manages to reproduce TBLG theoretical calculations reported in the literature in a good approximation. Dcp(ω, Tc) for TBLG suggests that Cooper-pairs are possible in the low-frequency vibration region. The calculations appear to be consistent with the existence of the superconducting state in TBLG, and it allows us to suggest the effect of the electric field for specific angles of rotation of TBLG.
Effects and challenges of public-health measures for zeroing out emerging contagions with varying transmissibility
Authors: Lai Shengjie , Ge Yong , Wu Xilin , Zhang Wenbin , Wang Xiaoli , Zhang Die , Wang Jianghao , Liu Haiyan , Ren Zhoupeng , Ruktanonchai Nick , Ruktanonchai Corrine W. , Cleary Eimear , Yao Yongcheng , Wesolowski Amy , Cummings Derek A.T. , Li Zhongjie , Tatem Andrew
Published: April 2023

Targeted public health interventions for an emerging epidemic are essential for preventing pandemics and reducing their impact on health and society. During 2020–2022, China invested significant efforts in strict zero-COVID policies to contain outbreaks of varying scales caused by different SARS-CoV-2 variants. This presented a unique opportunity to utilise a real-world dataset to investigate the effects and challenges of public health interventions aimed at eliminating local transmission of emerging or introduced respiratory infections in diverse settings. Based on a multi-year empirical dataset containing 131 outbreaks observed in China from April 2020 to May 2022 and simulated scenarios, we ranked the relative effectiveness of the public health interventions by their reduction in instantaneous reproduction number. We found that, overall, reducing physical contact between individuals was most effective (median 23%, interquartile range [IQR] 20–26%), followed by the use of face masks (19%, IQR 16–23%) and close contact tracing (15%, IQR 14–16%). However, contact tracing played a more critical role in containing outbreaks during the initial phases, but as the spread persisted, social distancing measures became increasingly prominent and mass screening likely had little effect. In addition, these measures struggled to zero out infections that had high transmissibility and a short latent period, regardless of population size. Our findings provide quantitative insights on the impacts of different rapid public-health responses against emerging contagions with varying epidemiological and socio-economic contexts.
An Analysis of FPGA LUT Bias and Entropy for Physical Unclonable Functions
Authors: Jao Jenilee , Wilcox Ian , Thotakura Sriram , Chan Calvin , Plusquellic Jim , Paskaleva Bilana S , Bochev Pavel B
Published: April 2023

Process variations within Field Programmable Gate Arrays (FPGAs) provide a rich source of entropy and are therefore well-suited for the implementation of Physical Unclonable Funtions (PUFs). However, careful considerations must be given to the design of the PUF architecture as a means of avoiding undesirable localized bias effects that adversely impact randomness, an important statistical quality characteristic of a PUF. In this paper, we investigate a ring-oscillator (RO) PUF that leverages localized entropy from individual look-up table (LUT) primitives. A novel RO construction is presented that enables the individual paths through the LUT primitive to be measured and isolated at high precision and an analysis is presented that demonstrates significant levels of localized design bias. The analysis demonstrates that delay-based PUFs that utilize LUTs as a source of entropy should avoid using FPGA primitives that are localized to specific regions of the FPGA, and instead a more robust PUF architecture can be constructed by distributing path delay components over a wider region of the FPGA fabric. Compact RO PUF architectures that utilize multiple configurations within a small group of LUTs are particularly susceptible to these types of design-level bias effects. The analysis is carried out on data collected from a set of identically-designed, hard macro instantiations of the RO implemented on 30 copies of a Zynq 7010 SoC.
Histone Deacetylase Inhibitors inhibit lung adenocarcinoma metastasis via HDAC2/YY1 mediated downregulation of Cdh1
Authors: Wang Dongmei , Yang Yixiao , Cao Yuxiang , Meng Meiyao , Wang Xiaobo , Zhang Zhengxun , Fu Wei , Duan Shichao , Tang Liming
Published: April 2023

Metastasis leads to high mortality of lung adenocarcinoma patients. Histone deacetylases are reliable targets of anti-tumor drugs, and histone deacetylase inhibitors (HDACi) have become a hot field of anti-tumor drug research. However, the specific mechanisms by which HDACi inhibits lung cancer metastasis have not been fully elucidated. Here, we detected the role of HDACi and HDAC2/YY1 in the process of lung adenocarcinoma migration by qPCR, immunobloting, co-immunoprecipitation, chromatin-immunoprecipitation and cell migration assay, combined with online database analysis. We found both trichostatin A (TSA) and sodium butyrate (NaBu) inhibit lung cancer cell invasion and migration significantly via Histone Deacetylase 2 (HDAC2). HDAC2 overexpression promotes lung cancer cell migration, while shHDAC2 inhibits lung cancer cell migration obviously. Further investigation show that HDAC2 interacts with YY1 and deacelytes Lycine 27 of Histone 3 to inhibits Cdh1 transcriptional activity and then promotes cell migration. This study revealed a new functional mechanism of HDAC2/YY1 in lung adenocarcinoma cell migration.
Clinical control study of acute idiopathic abducens nerve palsy treated with injections of dexamethasone into the lateral rectus muscle
Authors: Shi Minghua , Qin Aijiao , Cheng Jing , Ren Hongxing , Chen Simin , Xing Yiqiao
Published: April 2023

PURPOSE:
To explore the efficacy and safety of local injections of dexamethasone into the lateral rectus muscle for treatment of acute idiopathic abducens nerve palsy (AIANP).
METHODS:
A study group and a control group were established for this study. The former received dexamethasone injections into the paralyzed lateral rectus muscle in combination with oral mecobalamin tablets. The control group only received mecobalamin tablets for no more than two months. The follow-up period was > 6 months. Incidence of lateral rectus palsy and diplopia were recorded, as well as complications.
RESULTS:
Between September 2019 and September 2022, a total of 72 patients with AIANP were included in this study (mean age: 54.1 ± 15.8 y). After treatment, all 39 patients in the study group were successfully treated (100%), while only 27/33 (81.8%) patients in the control group were successfully treated. This difference between groups was statistically significant (Fisher’s exact test, P = 0.01). Recovery times for the two groups also differed (3.45 ± 1.49 weeks vs. 12.1 ± 9.83 weeks, respectively; P < 0.001). Among the two groups, only one case of conjunctival congestion was reported at the injection site in the treatment group. Furthermore, recurrence was not observed during the follow-up period (17.9 ± 8.17 months).
CONCLUSION:
Dexamethasone injections into the lateral rectus muscle of AIANP patients are found to be a safe and effective treatment. They also improved the treatment rate and shortened treatment time．
An infant with hand dorsal compartment syndrome due to intravenous infiltration of glucose-acetate Ringer's solution
Authors: Tomori Shinya , Korematsu Seigo , Momose Taichi , Urushihara Yasuko , Moriwaki Koichi
Published: April 2023

Introduction: Compartment syndrome caused by infusion pump in children has not been sufficiently reported.
Case presentation: A five-month-old boy was admitted to the hospital and underwent decompression of a dorsal hand compartment while receiving only glucose-acetate Ringer's solution.
Conclusion: Infiltration, a commonly seen complication with intravenous fluids and their administration, can cause compartment syndrome due to a variety of factors and should be treated promptly when infiltration or extravasation is detected. Guidelines and recommendations for the management of such cases have not been sufficiently described, and specific guidelines to prevent and detect fluid leakage early in infants are necessary.
Advanced Operation of Heated Fluidic Resonators via Mechanical and Thermal Loss Reduction in Vacuo
Authors: Lee Jungchul , Ko Juhee , Lee Bong Jae
Published: April 2023

For simultaneous and quantitative thermophysical properties measurements of ultra-small liquid volume, we have recently developed and reported heated fluidic resonators (HFRs). In this paper, we improve the precision of HFRs in vacuum by significantly reducing thermal loss around the sensing element. A vacuum chamber with optical, electrical, and microfluidic access is custom-made to decrease the convection loss by two orders of magnitude at 10-4 mbar. As a result, measurement sensitivities for thermal conductivity and specific heat capacity are increased by 4.1 and 1.6 times, respectively. Upon differentiation between deionized water (H2O) and heavy water (D2O) exhibiting similar thermophysical properties but ~10% different mass density, signal-to-noise ratios, property difference between H2O and D2O divided by the standard error, are increased by 9 and 5 times for thermal conductivity and specific heat capacity, respectively.
Downregulation of TAB182 promotes cancer stem-like cell properties and therapeutic resistance in triple-negative breast cancer cells
Authors: He Huan , Wang Shaozheng , Zhang Wen , Gao Shanshan , Guan Hua , Zhou Pingkun
Published: April 2023

Table 182 participates in DNA damage repair and radio-/chemo-sensitivity regulation in various tumors, but its role in tumorigenesis and therapeutic resistance of breast cancer remains unclear. In the current paper, we observed that Triple-Negative Breast Cancer (TNBC), a highly aggressive type of breast cancer, exhibits a lower expression of TAB182. TAB182 knockdown stimulates cell proliferation, migration, and invasion in TNBC cells. Our study first constructed the RNA-seq data to explore the cellular functions of TAB182 at the genome level in TNBC cells. Transcriptome analysis and in vitro experiments identify that TAB182 downregulation drives the enhanced properties of cancer stem-like cells (CSCs) in TNBC cells. Furthermore, TAB182 deletion contributes to the resistance of cells to Olaparib or Cisplatin, which could be rescued by silencing GLI2, a downstream gene of cancer stemness-related signaling pathways. Our results reveal a novel function of TAB182 as a significant negative regulator of cancer stem-like properties and drug sensitivity in TNBC cells, suggesting TAB182 may be a tumor suppressor gene and associated with more therapeutic benefits for TNBC.
Development of Automatic Orbital Pipe
MIG Welding System and Process
Parameters Optimization of AISI 1020 Mild
Steel Pipe Using Hybrid Artificial Neural
Network and Genetic Algorithm
Authors: Mengistie Amanuel Kassa , Bogale Teshome Mulatie
Published: April 2023

Currently, in pipe welding, it is nearly difficult for a human welder to weld
the whole circumference of a pipe in a single uninterrupted pass using
MIG welding causing inconsistencies in weld quality around the welded
pipe. The aim of this study was to develop an automated-orbital pipe
MIG welding system and to optimize welding parameters for enhancing
ultimate tensile strength and Rockwell hardness of mild steel 1020 grade
pipe. Three levels of variation were applied to the four input parameters
that were chosen. Nine experiments were carried out using orthogonal
array of L9. In this experimental investigation, the highest ultimate tensile
strength (UTS) of 411.2 MPa and Rockwell hardness (RH) of 95
HRB were achieved at 110 A of current and 24 V of voltage, welding
gun travel speed of 30 cm/min and 3 mm of arc length. For modeling
the orbital pipe MIG welding process experimental input parameters and
response results, a hybrid ANN-GA model was constructed. This model
was used to forecast and optimize ultimate tensile strength and Rockwell
hardness, as well as the process factors that go with it. The results
indicated that the ANN-GA model could predict the output responses
with a mean absolute error of 5.06e-05. During optimization, a 4-9-2
network trained with neural network of back propagation by Bayesian
regularization approach was determined to have the greatest prediction
capability, with maximum UTS and RH of 417.857 MPa and 96.5364
HRB, respectively. The predicted and confirmation test results were
both found within the acceptable errors, according to a confirmation
test conducted with the optimum parameters of the ANN-GA model.
Development of a spectrophotometric method for the quantification of c-phycocyanin in the cyanobacteria Aphanizomenon flos-aquae
Authors: Billy Julie , Pruvost Jeremy , Lépine Olivier , Drouin Delphine , Gonçalves Olivier
Published: April 2023

The current study presents the development of a reliable method for the quantification of c-phycocyanin. It was found that the spectrophotometric method commonly used for c-phycocyanin quantification tends to overestimate the actual amount of c-phycocyanin in AFA samples. Thus, the aim of this study was to account for c-phycocyanin variation between cyanobacteria species in order to reliably adapt the spectrophotometric quantification method of c-phycocyanin for Aphanizomenon flos-aquae (AFA). High performance liquid chromatography (HPLC) was used to avoid interference between molecules. The existing spectrophotometric equations for the quantification of AFA c-phycocyanin were adapted using a c-phycocyanin standard. The method was then used to obtain a new set of spectrophotometric quantification equations that were adapted to the strain of interest and ensured the accuracy of c-phycocyanin quantification while continuing to use a rapid, simple, and inexpensive method for pigment quantification. The method developed here could be adapted to improve the quantification methods for other types of phycocyanin, cyanobacteria, or even other compounds of interest that are currently quantified by spectrophotometry.
Spatial genetic structure of two conifers in a highly modified landscape of central Mexico
Authors: Cruz-Salazar Bárbara , Flores-Manzanero Alejandro
Published: April 2023

Forests have been globally reduced due to unprecedented rates of deforestation and habitat transformation, which in turn affects the species’ genetic structure. Despite harboring a high endemism, temperate forests are the second most degraded terrestrial ecosystem in Mexico. Therefore, understanding the spatial genetic patterns is fundamental to implement management and conservation efforts that contribute with their long-term persistence. By using Single Nucleotide Polymorphisms, this study evaluated the fine-scale spatial genetic structure and the effect of landscape features on gene flow of two conifers, Abies religiosa and Pinus montezumae, each with different successional affinity, in a temperate forest of central Mexico exhibiting high landscape heterogeneity due to topography and land-use changes. Based on successional affinity of species we expected isolation by resistance in Abies religiosa and a panmictic population in Pinus montezumae. We analyzed the genetic structure and the effects of altitude, Aspect and land-use changes on the genetic connectivity patterns. A weak isolation by distance pattern was detected in A. religiosa, while the spatial component explained only the 3% of its genetic variation. In P. montezumae, a very low structure signal was detected between slopes. Overall, the analyses revealed an absence of genetic structure in both species, i.e., no landscape predictor had an effect on gene flow between the populations. We recommend increasing the spatial extent and the assessment of more environmental predictors to further support the study of genetic structure patterns in the species studied. Our study contributes with information for the conservation of Mexican temperate forests.
Valorisation of buttermilk in mixotrophic and heterotrophic conditions by using the acidophilic strain Galdieria sulphuraria
Authors: Occhipinti Paride Salvatore , Signore Flavia Del , Canziani Stefano , Caggia Cinzia , Mezzanotte Valeria , Ferrer-Ledo Narcís
Published: April 2023

The growth of the polyextremophile red microalga Galdieria sulphuararia ACUF064 under mixotrophy and heterotrophy was evaluated in a lab-scale stirred photobioreactor, using buttermilk as carbon source. Beforehand, G. sulphuraria growth on glucose, galactose and lactose, was evaluated. The final biomass concentration of G. sulphuraria was about 2 and 1.5 times lower in mixotrophy and heterotrophy, respectively, when G. sulphuraria ACUF064 was grown on lactose rather than in glucose or galactose. No significant difference was observed in mixotrophy between growth in glucose and galactose. C-phycocyanin content was about 1.6 and 1.37 times higher in mixotrophy on lactose than on galactose and on glucose. In heterotrophy, C-phycocyanin content, on lactose was 1.57 and 1.56 times higher than values detected in glucose and galactose, respectively. The preliminary mixotrophic flask experiment with different buttermilk dilutions showed that the highest biomass production was obtained at 1:2.5 dilution rate, corresponding to 40% v/v of butter milk and to 2.5 gC L− 1. On buttermilk, the highest biomass production was obtained in mixotrophy, at 1:2.5 dilution rate, in a 13 L photobioreactor where the algal productivity was 0.55 g L− 1 d− 1, and no contamination occurred at pH 1.6.
Dynamic performance of newly developed environmentally friendly greases containing polysaccharide gums in rolling bearing
Authors: Saxena Ankit , Kumar Deepak , Tandon Naresh
Published: April 2023

The present study explores the performance of novel vegetable oil-based greases containing polysaccharide gums as additives. Two series of greases with varying concentrations of gum acacia (GA) and guar gum (GG) are evaluated in a rolling bearing at various speeds and loads. The vibrations and shock pulse measurements (SPM) quantified the tribo-dynamics of the developed greases against two commercial greases used as the benchmark. The vibration and SPM assessment suggest that the newly developed eco-friendly greases are at par with the commercial ones; the performance varies with the concentration and type of gum. Where a cumulative effect of rheology and gum size seems to influence the vibrations, the entrainment of gums in the contact zone governs the SPM carpet values. The developed greases show great prospects as a sustainable, potential, and commercial alternative to existing environmentally unfriendly greases.
Serum metabolic signatures for Alzheimer’s Disease reveal alterations in amino acid composition and energy metabolism – A validation study
Authors: Nielsen Jonas Ellegaard , Andreassen Trygve , Gotfredsen Charlotte Held , Olsen Dorte Aalund , Vestergaard Karsten , Madsen Jonna Skov , Kristensen Søren Risom , Pedersen Shona
Published: April 2023

Background: Alzheimer’s Disease (AD) is complex and novel approaches are urgently needed to characterise disease pathology and to aid in diagnosis. Metabolites are the end-products of upstream molecular alterations, whereby small changes at the genetic or protein level result in substantial changes at the metabolite level. Blood is frequently used as a source for biomarkers; however, its complexity prevents proper detection. The analytical power of metabolomics, coupled with statistical tools, can assist in reducing this complexity. Furthermore, the current bottleneck in biomarker research is reproducibility and appropriate validation. Thus, we sought to validate a previously proposed panel of metabolic blood-based biomarkers for AD and expand our understanding of the pathological mechanisms involved in AD that are reflected in the blood.
Methods: In the validation cohort serum and plasma were collected from 25 AD patients and 25 healthy controls. Serum was analysed for metabolites using nuclear magnetic resonance (NMR) spectroscopy, while plasma was tested for markers of neuronal damage and AD hallmark proteins using single molecule array (SIMOA). A combination of multivariate and univariate statistics were utilized to validate established biomarkers and uncover new disease-related evidence.
Results: The diagnostic performance of the proposed metabolite biomarker panel was confirmed using sparse-partial least squares discriminant analysis (sPLS-DA) with an area under the curve (AUC) of 0.89 (95 % confidence interval: 0.79 – 0.98). Five metabolites (pyruvic acid, valine, leucine, histidine, and isoleucine) were consistently reduced in both the discovery and validation cohorts. Pathway analysis of significantly altered metabolites in the validation set revealed that they are involved in branched-chain amino acids (BCAAs) and energy metabolism (glycolysis and gluconeogenesis). Additionally, a moderate correlation was observed between valine and the proteins neurofilament light and glialfibrillary acidic protein. By combining the significant protein expression levels measured by SIMOA with the sPLS-DA model, the AUC increased to 0.97 (95 % CI: 0.93 – 1.00).
Conclusions: Our proposed panel of metabolites was successfully validated using a combined approach of NMR and sPLS-DA. It was discovered that cognitive-impairment-related metabolites belong to BCAAs and are involved in energy metabolism.
Lautropia mirabilis-induced peritoneal dialysis-associated peritonitis: a case report
Authors: Liu Shuhui , Yan Yufen , Hu Changcan , Huang Xiuling , Liu Qing , Liu Yanfei , Jia Nan , Han Chunhua
Published: April 2023

Background
Lautropia mirabilis is a Gram-negative bacterium mainly isolated from the mouth and upper respiratory tract. To date, only two cases of peritoneal dialysis-associated peritonitis induced by Lautropia mirabilis have been globally reported: one in Australia and one in Portugal, but Lautropia mirabilis has not been cultured. Here, we report the third case of peritoneal dialysis-associated peritonitis caused by Lautropia mirabilis in the world. Furthermore, we cultivated this bacterium to improve the existing knowledge on the etiology of this condition and provide clinical guidance for its treatment.
Case presentation:
A 49-year-old female with renal insufficiency for more than five years was admitted to our hospital. She had been on peritoneal dialysis for six months. The patient had diarrhea and turbid peritoneal dialysis fluid for three days. With indication of infection by routine results of peritoneal dialysis fluid, mass spectrometer identification and gene sequencing of peritoneal dialysis fluid culture were subjected and revealed infection with Lautropia mirabilis. After anti-infection treatment with gentamicin and ceftazidime, the patient recovered.
Conclusions
Our findings suggest that Lautropia mirabilis plays an important role in the pathogenesis of peritoneal dialysis-associated peritonitis. Early diagnosis and timely antibiotic treatment may be essential for the treatment of this infection.
Vacuum Assisted Closure Therapy in Children with Complicated Abdominal Wounds, with or without Viscero-Cutaneous Fistulae
Authors: ghandour mostafa el , naggar osama el , radwan ahmed , abdel-latif mohammed
Published: April 2023

Background:Management of complicated abdominal wounds is challenging in children. Vacuum-assisted closure proved to be beneficial. We aimed to report the outcome of VAC over 5 years’ practice in complicated abdominal wounds with or without viscero-cutaneous fistulae.
Methods:Retrospective review of children managed by VAC over 5 years, from January 2017 to December 2022. Included patients had complicated abdominal wounds. Customized VAC devices with continuous negative pressure were used for all patients. The applied pressure was tailored according to the patient’s age and the wound condition.
Results:Nineteen patients were included, their ages ranged from 3 months to 15 years. Peritonitis was the main pathology in 12 patients, abdominal wall infection in 4, and a combination of both in 3. VAC was applied over closed wounds in 10 patients and over open wounds in 9; 6 of them had cutaneous fistulae. The median duration of VAC application over closed wounds was 7.5 days ,while it was 10 days in open wounds. VAC promoted healing in closed and narrow-gapped wounds, while it was successfully used as a bridging therapy in 4 patients with wide-gapped wounds. Patients with viscero-cutaneous fistulae were successfully managed without further surgical intervention. VAC was complicated by fascial dehiscence with evisceration and incisional hernia in two patients.
Conclusion:VAC could be beneficial to complicated abdominal wounds in pediatric age. It was helpful in managing small viscero-cutaneous fistulae.
Interleukin-17 regulates keratinocyte proliferation in psoriasis through the MALAT1/miR-125b/ BRD4 axis
Authors: Pan Min , Zhu Xiao-fang , Chen Yi-he , Lin Xiang-fei , Wang Xiaokang
Published: April 2023

Background: Psoriasis is a common chronic inflammatory dermatosis characterized by scaly erythema and plaques. This study explored the specific mechanism of LncRNA MALAT1 in the occurrence and development of psoriasis.
Methods: We collected serum from psoriasis patients and healthy subjects and measured the expression of IL-17 in serum by ELISA. HaCaT cells were stimulated with 80 ng/mL IL-17 and simultaneously transfected with shMALAT1 or were treated with Secukinumab (anti-IL-17A). The CCK-8 and EdU experiments were used to detect cell viability and proliferation, flow cytometry showed the level of cell cyle, and corresponding cyclins were measured by WB and RT-QPCR, and changes in a series of LncRNAs were detected. A luciferase activity analysis and ChIP assay verified the targeting relationship between MALAT1 and p65.
Results: IL-17 treatment of HaCaT cells promoted cell proliferation and increased MALAT1 expression. The binding of p65 to the promoter of MALAT1 promoted keratinocyte proliferation. The proliferation of HaCaT cells induced by IL-17 was repressed by Secukinumab. The expression of miR-125b was notably reduced in cells treated with IL-17, whereas Secukinumab rescued the expression of miR-125b. IL-17 treatment resulted in elevated BRD4 expression, which was attenuated by Secukinumab.
Conclusions: IL-17 stimulates HaCaT cell proliferation by inducing high expression of MALAT1, and p65 binds to the promoter of MALAT1 to promote its expression, which may provide a novel direction for the treatment of psoriasis.
Research on the relationship between asset structure and enterprise growth ability of listed automobile companies: Evidence from China
Authors: Jiang Allen
Published: April 2023

In China's national economy, the auto industry has played a crucial role in stimulating the economy and promoting employment. Assets are the foundation of the production and operation activities of automobile manufacturing enterprises. Different asset structures (AS) will bring different levels of income and risk, and there will be differences in the impact on the growth of enterprises. This paper constructs a comprehensive index evaluation system of enterprise growth ability (GA) with 17 evaluation indicators in six dimensions: profitability, operating ability, risk response-ability, market growth ability, innovation ability and human capital. The variables of AS are determined from the two dimensions of total AS and current AS, and the relationship between AS and enterprise GA is tested by multiple regression analysis. This study found that the proportion of current assets is significantly positively correlated with the enterprise GA in listed companies in China's auto industry in the total AS. The proportion of fixed assets is negatively correlated with the enterprises GA. The proportion of intangible assets is slightly negatively correlated with the GA of current enterprises, and significantly positively correlated with the future GA. In the current AS, the proportion of monetary capital is significantly positively correlated with the GA of enterprises, while the proportion of inventory is significantly negatively correlated with the GA of enterprises.
PointCMC: Cross-Modal Multi-Scale Correspondences Learning for Point Cloud Understanding
Authors: Zhou Honggu , Peng Xiaogang , Luo Yikai , Wu Zizhao
Published: April 2023

Existing cross-modal frameworks have achieved impressive performance in point cloud representation learning, where a 2D image encoder is employed to transfer knowledge to a 3D point cloud encoder. However, the local structures between point clouds and corresponding images are unaligned, which results in a challenge for the 3D point cloud encoder to learn fine-grained image-point cloud interactions. In this paper, we introduce a novel multi-scale training strategy (PointCMC) to enhance fine-grained cross-modal knowledge transfer in the cross-modal framework. Specifically, we design a Local-to-Local (L2L) module that implicitly learns the correspondence of local features by aligning and fusing extracted local feature sets. Moreover, we introduce the Cross-Modal Local-Global Contrastive (CLGC) loss, which enables the encoder to capture discriminative features by reasoning local structures to their corresponding cross-modal global shape. The extensive experimental results demonstrate that our approach outperforms the previous unsupervised learning methods in various downstream tasks such as 3D object classification and semantic segmentation.
Brain metastases in adult patients with melanoma of unknown primary in the Netherlands (2011–2020)
Authors: Padilla C. S. , Ho V. K.Y. , Mooijenkind T. W.A.N. , Louwman M. W.J. , Vos F. Y. F. L , Bekkenk M. W. , Minnaard W. F . , Loef C. , Zanten S.E.M Veldhuijzen van
Published: April 2023

Background
Although patients with melanoma of unknown primary (MUP) have a better prognosis than similar-staged melanoma patients with known primary, the occurrence of brain metastases (BM) entails a serious complication. This study provides an overview of the incidence, treatment patterns, and overall survival (OS) of adult patients with BM-MUP in the Netherlands.
Methods
BM-MUP cases were retrieved from the Netherlands Cancer Registry. Patient, disease and treatment-related characteristics were summarised using descriptive statistics. Overall survival (OS) was calculated by the Kaplan-Meier method, and the impact of prognostic factors on OS was assessed using Cox proportional hazard regression analyses.
Results
Among 1,779 MUP patients, 450 were identified as BM-MUP (25.3%). Of these patients, 381 (84.7%) presented with BM along with other metastases, while 69 (15.3%) had BM only. BM-MUP patients were predominantly male (68.2%), and had a median age of 64 years at diagnosis (interquartile range 54–71 years). Over time, the proportion of BM along other metastatic sites increased, and the occurrence of BM decreased (p = 0.01). 1-Year OS improved for the total population, from 30.0% (95% confidence interval (CI): 19.8–40.9%) in 2011–2012 to 43.6% (95%CI: 34.5–52.3%) in 2019–2020, and median OS more than doubled from 4.2 months (95%CI: 3.3–6.2 months) to 9.8 months (95%CI: 7.0–13.2 months). Patient’s age, localisation of BM, presence of synchronous liver metastasis and treatment were identified as independent predictors of OS.
Conclusion
Notwithstanding the progress made in OS for patients with BM-MUP in the past decade, their overall prognosis remains poor, and further efforts are needed to improve outcomes.
Leukemia relapse via genetic immune escape after allogeneic hematopoietic cell transplantation
Authors: Pagliuca Simona , Gurnari Carmelo , Hercus Colin , Hergalant Sébastien , Hong Sanghee , Dhuyser Adele , D’Aveni Maud , Aarnink Alice , Rubio Marie Thérèse , Feugier Pierre , Ferraro Francesca , Carraway Hetty E. , Sobecks Ronald , Hamilton Betty K. , Majhail Navneet S. , Visconte Valeria , Maciejewski Jaroslaw P.
Published: April 2023

Graft-versus-leukemia (GvL) reactions are responsible for the effectiveness of allogeneic hematopoietic cell transplantation as a treatment modality for myeloid neoplasia, whereby donor T- effector cells recognize leukemia neoantigens. However, a substantial fraction of patients experience relapses because of the failure of the immunological responses to control leukemic outgrowth. Here, through a broad immunogenetic study, we demonstrate that germline and somatic reduction of human leucocyte antigen (HLA) heterogeneity enhances the risk of leukemic recurrence. We show that preexistent germline-encoded low evolutionary divergence of class II HLA genotypes constitutes an independent factor associated with disease relapse and that acquisition of clonal somatic defects in HLA alleles may lead to escape from GvL control. Both class I and II HLA genes are targeted by somatic mutations as clonal selection factors potentially impairing cellular immune reactions and response to immunomodulatory strategies. These findings define key molecular modes of post-transplant leukemia escape contributing to relapse.
Leaf, Root, and Crown Tissue Physiology of Annual Bluegrass after Cold Acclimation at Varying Soil Moisture Levels and Ice Encasement
Authors: Gendjar Megan , Merewitz Emily
Published: April 2023

Annual bluegrass (Poa annua) is a turfgrass species prone to winterkill-induced damage such as from ice encasement stress. This research aimed to determine whether different levels of soil volumetric water content (SWC) influence cold acclimation and recovery from ice encasement. Annual bluegrass was exposed to 8%, 12%, and 20% SWC treatments during cold acclimation in growth chambers. After cold acclimation, plants were subjected to ice encasement by misting at –3 °C until a 2.5-cm ice layer was formed. On 0 (no ice encasement exposure), 40, and 80 days of treatment, plants were analyzed for recovery (percent green canopy cover over time), and leaf, crown, and root tissues were harvested for lipid peroxidation and total nonstructural carbohydrates (TNC) including storage carbohydrates and water-soluble carbohydrates (WSC). Low SWC during cold acclimation enhanced recovery from cold temperatures and ice encasement. Root carbohydrates were influenced by SWC regimes during cold acclimation since day 0 plant roots exposed to the 8% SWC treatment had 143.9% higher TNC and 137.6% higher WSC compared with day 0 plants exposed to 12% and 20% SWC. Root levels of carbohydrates and lipid peroxidation were least influenced by cold and ice encasement among the organs evaluated. Prolonged freezing conditions and ice encasement reduced leaf and crown tissue carbohydrates and increased lipid peroxidation compared with day 0 plants not exposed to freezing temperatures and ice encasement. After 40 days of ice encasement, plants exposed to the 8% SWC treatment recovered faster than plants cold acclimated at higher soil moisture levels. Average percent canopy cover after 36 days of recovery in the greenhouse was 71.9% higher for 8% SWC treated plants than in 12% and 20% SWC treated plants. Turfgrass managers may benefit from annual bluegrass putting green management strategies to reduce fall soil moisture. Given that soil moisture did not significantly influence carbohydrate or lipid peroxidation results, except for in roots, additional research is needed to understand the mechanism associated with these findings.
Dietary Habits and Meal Pattern of People Residing in Anand District during COVID-19 Lockdown
Authors: Roghelia Viraj , Patel V. H.
Published: April 2023

Background: Coronavirus disease-2019 (COVID-19) is a condition caused by the novel coronavirus SARS-CoV-2. It was declared as global pandemic as it affected millions of people worldwide. A nationwide lockdown was announced to prevent the spread of the disease in India on March 24, 2020. The sudden change in routine lifestyle, home confinement, and fear of this pandemic caused stress and anxiety among the people and this has affected many habits of the people. Objective: The present study was conducted to evaluate changes in the dietary habits and meal pattern of people residing in Anand district during COVID-19 lockdown. Methodology: An online survey was conducted through semi-structured questionnaire to study the dietary habits and meal consumption pattern before and during COVID-19 lockdown. Result: The result showed a change in the regularity of meal consumption, type, and number of meals consumed by the respondents. More than 25% of respondents have reported an increase in the number of meals cooked, quantity of food cooked, and number of food items in one meal during the lockdown. Moreover, higher consumption of fruits and vegetables, protein rich foods, herbs, spices, and limited use of fats, oils, sugar, and salt was reported by more than 30% respondents. Conclusion: The present study concludes that this shift toward healthier food habits if sustained could have positive effects on health of people.
A Comparative Study of Self-esteem among Native and Migrant Adolescents in Sultanpur District
Authors: Patel Pinka , Kiran U. V. , Santoshi K. M.
Published: April 2023

Background: Adolescence is the age from childhood to adulthood; it is a period of physical and psychological growth and development. Self-esteem tends to be lowest in childhood and increases with age. Self-esteem affects the decision-making process, relationships, and emotional and mental well-being. People with low self-esteem may not feel motivated to try some new things, and they may also have problems with their relationships and expressing themselves. Aim: The purpose of this comparative study is to investigate the self-esteem development among native and migrant adolescents. Materials and Methods: For the study, 120 subjects were selected randomly from different areas of Sultanpur District. A self-structured interview schedule and the Rosenberg Self Esteem Scale were used for data collection and analysis. Results: The study clearly revealed that native and migrant adolescents exhibited differences while trying new things, taking new challenges, and having an overall opinion about themselves. Conclusion: Adolescents have varying levels of self-esteem, which appears to be influenced by such factors as age, gender, social class, and residential status. The results of the present study reveal that the self-esteem of native adolescents is higher than that of migrant adolescents. This may be due to the fact that native adolescents are more secure than migrant adolescents.
Exploring the Impact of Electronic Health on Total Quality Management in Jordanian Hospitals: The Mediating Role of Knowledge Management Technology
Authors: Bodoor Nora , Al-Kasasbeh Mohammed Mufaddy
Published: April 2023

The aim of this study is to measure the impact of electronic-health (e-health) on total quality management (TQM) through knowledge management (KM) technology as a mediating variable at the hospitals of Irbid Governorate in Jordan. Six elements of the e-health variable were considered, including e-health records, e-data transmission, e-medical prescriptions, video consultations, medical image sharing, and continuous medical learning. A questionnaire was designed and distributed to a sample of 600 individuals working at 15 different hospitals that apply an e-health system in the Irbid Governorate in Jordan; 566 questionnaires were deemed valid for statistical analysis. The results of this study show that there is a statistically significant impact of e-health on TQM, at level P ≤ 0.05, through the mediating role of KM technology at Irbid Governorate hospitals in Jordan. Although the data were collected from hospitals at the Irbid Governorate in Jordan only, it is hoped the results will have some validity for the rest of hospitals in the Jordanian health system. The most significant recommendation of the study with practical implication is to set TQM as an administrative approach at the hospitals of the governorate of Irbid in Jordan, and require the adoption and implementation of an e-health system as a requirement for all Jordanian hospitals to acquire local accreditation certificate with the overall goal of improving the quality of healthcare.
Antibacterial Activity of Justicia betonica Linn
Authors: Naik Swasha Khandeparker , Manjula B. L. , Balaji M. Vaidagi , Marndi Sugimani , Kumar Sanjeet , Devi Rajkumari Supriya
Published: April 2023

Justicia betonica L. (Acanthaceae) is widely used as a traditional folk medicinal herb. Conventionally, the plant is used to cure constipation, diarrhea, malaria, pain, stomach ache, vomiting, etc. The plant possesses many therapeutic uses such as analgesic, anti-inflammatory, antimalarial, and antimicrobial properties. The present work has been designed to document the ethnomedicinal properties, phytochemistry, and antibacterial activity of J. betonica. Phytochemical results showed that the presence of wide variety of biologically active compounds such as flavonoids, alkaloids, tannins, and phenolic compounds might be responsible for therapeutic values of Justicia betonica. Antibacterial activity showed that the inhibition was observed with the individual extracts and was effective against all bacterial strains tested. The present study highlights the importance of J. betonica.