bluuebunny/crossref_metadata_2025 huggingface dataset:
Una lengua, una nación. Fronteras lingüísticas, sociales y políticas en dos casos de la migración latinoamericana al Reino Unido
Authors: Morales Hernández Francisco Daniel
Published: August 2022

This article explores one nation-one language ideology, which holds that a language is the glue and marker of an identity that fosters national unity and linguistic assimilation ideologies in the context of Latin American migration to the United Kingdom. From ethnographic and sociocognitive approaches within critical discourse studies, the linguistic experiences of two Latin American immigrant mothers seeking health services and legal advice are analysed. The study of their linguistic experiences sheds light on the social and political effects that language ideologies have by bringing about such consequences as physical aggression and family separation in underexplored institutions articulated by neoliberal policies. In this context, a nationalist ideology emerges that constructs borders and marginal social identities.
Atonal homolog 8/Math6 regulates differentiation and maintenance of skeletal muscle
Authors: Divvela Satya Srirama Karthik , Offei Eric Bekoe , Suerland Florian , Revuelta García David , Kwiatkowski Julia , Balakrishnan-Renuka Ajeesh , Bohne Pauline , Böing Marion , Morosan-Puopolo Gabriela , Mark Melanie D. , Brand-Saberi Beate
Published: August 2022

Atonal Homolog 8 (Atoh8) belongs to a large superfamily of transcriptional regulators called basic helix-loop-helix (bHLH) transcription factors. Atoh8 (murine homolog “Math6”) has been shown to be involved in organogenesis during murine embryonic development. We have previously identified the expression of Atoh8 during skeletal myogenesis in chicken where we described its involvement in hypaxial myotome formation suggesting a regulatory role of Atoh8 in skeletal muscle development. Within the current study, we analyzed the effect of the loss of function of Atoh8 in murine primary myoblasts and during differentiation of pluripotent stem cells into myotubes, and the effect of its gain of function in C2C12 cells. Based on the observed results, we conclude that Atoh8 regulates myoblast proliferation via modulating myostatin signaling. Further, our data revealed a reduced muscle mass, strength and fiber size with significant changes to the muscle fiber type suggesting atrophy in skeletal muscle of Atoh8 mutants. We further report that Atoh8 knockout mice suffer from a condition similar to ambient hypoxia which may be the primary cause of the phenotype. Altogether, this study shows the significance of Atoh8 not only in myogenesis but also in the maintenance of skeletal muscle.
Oral antibiotics perturbation on gut microbiota after prostate biopsy
Authors: Li Joseph Kai Man , Wang Lynn Lin , Lau Becky Su Yan , Tse Ryan Tsz Hei , Cheng Carol Ka Lo , Leung Steven Chi Ho , Wong Christine Yim Ping , Tsui Stephen Kwok Wing , Teoh Jeremy Yuen Chun , Chiu Peter Ka Fung , Ng Chi Fai
Published: August 2022

Introduction

The use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral co-amoxiclav before transrectal ultrasound-guided transperineal prostate biopsy (TPPBx) in 5 weeks’ time.

Methods

Fifteen patients with elevated serum prostate-specific antigen (PSA) were recruited to provide pre-antibiotic and post-antibiotic fecal samples. The V4 region of 16S rRNA was sequenced. Analysis was performed by QIIME2. Alpha- and beta-diversities were analyzed, as well as the differential enrichment by Linear discriminant analysis Effect Size (LEfSe) analysis.

Results

Both the alpha- and beta-diversities of the pre- and post-antibiotic fecal samples were significantly different. Genera that are associated with alleviation of inflammation were enriched in the pre-antibiotic fecal samples, while the inflammation-associated genera were more enriched in the post-antibiotic fecal samples.

Conclusion

A single dose of oral co-amoxiclav before TPPBx could have led to a change of gut microbiota that cannot be recovered in 5 weeks' time. Microbiome studies on prostate cancer patients should be cautioned on the use of post-prostate biopsy fecal sampling. Further studies should be conducted for the impact on gut microbiome for TPPBx alone.
Bear with me: Understanding motivations for bear farming in Vietnam
Authors: Green Jennah , Schmidt-Burbach Jan , Kukreja Karanvir , Guillon Elodie
Published: August 2022

Bears were once farmed legally across Vietnam to supply bile, a digestive fluid, as an ingredient for traditional medicine products. Extracting and selling bear bile has been prohibited in Vietnam since 2005, but there is evidence that an illegal industry remains active. The aim of this study is to gain insight into the motivations and experiences of Vietnamese bear farmers. Thirty-seven semi-structured interviews were conducted with 28 active and 9 former bear farmers across 14 provinces in Vietnam. Our findings confirm previous reports in the literature that there is still an active, illegal bear bile market in Vietnam, but that bear farming is becoming less profitable due to a reduction in demand for farmed products and the decreasing price of bile. Additional results indicate that many Vietnamese bear farmers may have a poor understanding of regulations governing the trade of bear parts and that most farmers do not intend to stop farming bears. Based on these findings, we suggest the most effective method of persuading bear farmers to stop trading bile would beviaformer bear farmers and to reduce the social acceptability of bile consumption throughout the country. We hope the results of this study can help refine strategies and inform future efforts to end the bear bile industry in Vietnam.
The silent trial - the bridge between bench-to-bedside clinical AI applications
Authors: Kwong Jethro C. C. , Erdman Lauren , Khondker Adree , Skreta Marta , Goldenberg Anna , McCradden Melissa D. , Lorenzo Armando J. , Rickard Mandy
Published: August 2022

As more artificial intelligence (AI) applications are integrated into healthcare, there is an urgent need for standardization and quality-control measures to ensure a safe and successful transition of these novel tools into clinical practice. We describe the role of the silent trial, which evaluates an AI model on prospective patients in real-time, while the end-users (i.e., clinicians) are blinded to predictions such that they do not influence clinical decision-making. We present our experience in evaluating a previously developed AI model to predict obstructive hydronephrosis in infants using the silent trial. Although the initial model performed poorly on the silent trial dataset (AUC 0.90 to 0.50), the model was refined by exploring issues related to dataset drift, bias, feasibility, and stakeholder attitudes. Specifically, we found a shift in distribution of age, laterality of obstructed kidneys, and change in imaging format. After correction of these issues, model performance improved and remained robust across two independent silent trial datasets (AUC 0.85–0.91). Furthermore, a gap in patient knowledge on how the AI model would be used to augment their care was identified. These concerns helped inform the patient-centered design for the user-interface of the final AI model. Overall, the silent trial serves as an essential bridge between initial model development and clinical trials assessment to evaluate the safety, reliability, and feasibility of the AI model in a minimal risk environment. Future clinical AI applications should make efforts to incorporate this important step prior to embarking on a full-scale clinical trial.
E-leadership of the school principals in implementing online learning during COVID-19 pandemic at public senior high schools
Authors: Indra Ristapawa , Ritonga Mahyudin , Kustati Martin
Published: August 2022

This research aimed to discover the implementation of e-leadership for school principals during the COVID-19 pandemic and its effect on school performance through changes in teacher behavior. Quantitative research was used where it involved 8 public high schools with 150 respondents. The results showed that: (1) the implementation of e-leadership for school principals during the COVID-19 pandemic was at a very high level for the dimensions of the vision of continuous learning and professional development of teachers, and at a high level for the dimensions of classroom supervision and teacher performance assessment; (2) none of the independent variables have a correlation with the dependent variable greater than >0.50. The correlation between the independent variable and the dependent variable is at a simple stage for teacher behavior and a low stage for teacher performance; (3) the result of measurement model test with Confirmatory Factor Analysis (CFA) shows that the loading factor of each indicator is greater than 0.60, which means that the indicators are valid for measuring the variable; (4) the results of the structural model test show that the principal’s e-leadership variable, scientific competence (pedagogical knowledge and pedagogical content knowledge), personality competence and e-skills have a positive and significant effect on teacher behavior with a value of ***p= < 0.05. Teacher performance also has a positive and significant effect with a value of ***p= < 0.05 which makes H-1–H-9 accepted. Proportionally, e-leadership remains the main variable compared to other variables which gives the largest contribution to improving teacher performance through changes in teacher behavior by (0.280), scientific competence variables (pedagogical knowledge and content knowledge) and teacher personality, respectively, contribute to improved performance through changes in teacher behavior of (0.083) and (0.152). The e-skill competency variable of teachers contributes to increased performance through changes in teacher behavior of (0.177). The practical implication of this research is that principals need to change their roles and leadership styles from conventional to electronic-based learning leadership styles to realize effective online learning.
Comparative analysis of bacterial communities in the sediment and seawater environments from marine large yellow croaker cages (Zhejiang coast, China)
Authors: Li Tiejun , Guo Dengkang , Shen Yuan , Bao Jingjiao , Jin Lei
Published: August 2022

Bacterial community plays a vital role in the open-oceanic aquaculture ecosystem, and its stability is crucial for maintaining the health of mariculture fish. However, there are no reported studies on microbial communities in the culture environment of marine fish cages. In this study, bacterial community composition and diversity of seawater and sediment habitats from 5 large yellow croaker cage farms were first investigated by 16S rRNA-based high-throughput sequencing. The composition of bacterial communities was visualized at the phylum and genus levels. Meanwhile, biological and ecological functions were also predicted. The bacterial diversity observed at 3 inshore sampling sites was higher in seawater groups than that in sediment groups. The alpha diversity indices were influenced by the geographic location, especially the inshore site Yueqing (YQ), showing higher values than other sampling sites. The pathogenic bacteria were prevalent in the aquaculture environments, and probiotics showed a relatively low proportion by comparing the potential pathogens. Our study provides baseline data on bacterial communities and diversity in the open-oceanic environments of cage-culture large yellow croaker.
Motoric cognitive risk syndrome and incident hospitalization in Quebec's older population: Results of the NuAge cohort study
Authors: Beauchet Olivier , Matskiv Jacqueline , Launay Cyrille P. , Gaudreau Pierrette , Allali Gilles
Published: August 2022

Background

Screening older adults at risk of hospitalization is essential to prevention of this adverse event. Motoric cognitive risk syndrome (MCR) has been associated with incident dementia and falls, which are both risk factors of hospitalization. There is no information on the association of MCR with incident hospitalization in older adults.

Objective

The study aims to examine the association of MCR with incident hospitalization in community-dwelling older adults.

Design

Quebec older population-based observational cohort study with 3 years of follow-up.

Setting

Community dwellings.

Subjects

A subset of 999 participants recruited in the NuAge study.

Methods

Participants with MCR (i.e., with slow gait and cognitive complaint without dementia or motor disability) were identified at baseline assessment. Incident hospitalization (i.e., ≥1) and its recurrence (i.e., ≥2) were collected annually over a 3 year follow-up period.

Results

The prevalence of MCR was 5.0% at baseline. The overall incidence of hospitalization was 29.0% and its recurrence 4.8%. MCR was associated with incident recurrent hospitalization [adjusted Hazard Ratio (aHR) = 2.58 with 95% Confidence Interval (CI) = (1.09–6.09) and P = 0.031], but not with incident hospitalization [aHR = 1.48, with 95%CI = (0.95–2.28) and P = 0.081].

Conclusion

MCR is associated with incident recurrent hospitalization in NuAge participants, suggesting that MCR may be of clinical interest for screening individuals at risk for hospitalization in Quebec's older population.
Identification of key biomarkers in Angelman syndrome by a multi-cohort analysis
Authors: Li Yong , Shu Junhua , Cheng Ying , Zhou Xiaoqing , Huang Tao
Published: August 2022

The Angelman Syndrome (AS) is an extreme neurodevelopmental disorder without effective treatments. While most patients with this disease can be diagnosed by genetic testing, there are still a handful of patients have an unrecognized genetic cause for their illness. Thus, novel approaches to clinical diagnosis and treatment are urgently needed. The aim of this study was to identify and characterize differentially expressed genes involved in AS and built potential diagnostic panel for AS by NGS sequencing. A multi-cohort analysis framework was used to analyze stem cell-derived neurons from AS patients in GSE160747 dataset. We identified three differentially expressed genes (ACTN1, ADAMTS2, SLC30A8) differentiates AS patients from controls. Moreover, we validated the expression patterns of these genes in GSE146640, GSE120225. Receiver operating characteristic (ROC) curves analysis demonstrated that these genes could function as potential diagnostic biomarkers [AUC = 1 (95% CI 1–1)]. This study may provide new approach for diagnosing patients with AS and helping to develop novel therapies in treating AS patients.
Neuroinflammation and neurodegeneration in diabetic retinopathy
Authors: Bianco Lorenzo , Arrigo Alessandro , Aragona Emanuela , Antropoli Alessio , Berni Alessandro , Saladino Andrea , Battaglia Parodi Maurizio , Bandello Francesco
Published: August 2022

Diabetic retinopathy (DR) is the most common complication of diabetes and has been historically regarded as a microangiopathic disease. Now, the paradigm is shifting toward a more comprehensive view of diabetic retinal disease (DRD) as a tissue-specific neurovascular complication, in which persistently high glycemia causes not only microvascular damage and ischemia but also intraretinal inflammation and neuronal degeneration. Despite the increasing knowledge on the pathogenic pathways involved in DR, currently approved treatments are focused only on its late-stage vasculopathic complications, and a single molecular target, vascular endothelial growth factor (VEGF), has been extensively studied, leading to drug development and approval. In this review, we discuss the state of the art of research on neuroinflammation and neurodegeneration in diabetes, with a focus on pathophysiological studies on human subjects, in vivo imaging biomarkers, and clinical trials on novel therapeutic options.
Acupuncture modulates the frequency-specific functional connectivity density in primary dysmenorrhea
Authors: Liu Li-Ying , Li Xiang , Tian Zi-Lei , Zhang Qi , Shen Zhi-Fu , Wei Wei , Guo Xiao-Li , Chen Ling , Su Meng-Hua , Yang Lu , Yu Si-Yi , Yang Jie
Published: August 2022

Background

The study aimed to investigate how acupuncture modulates brain activities across multiple frequency bands to achieve therapeutic effects in PDM.

Methods

A total of 47 patients with PDM were randomly assigned to the verum acupuncture group and sham acupuncture group with three menstrual cycles of the acupuncture course. The fMRI scans, visual analog scale (VAS) scores, and other clinical evaluations were assessed at baseline and after three menstrual-cycles treatments. The global functional connectivity density (gFCD) analyses were performed between the pre-and post-acupuncture course of two groups at full-low frequency band, Slow-3 band, Slow-4 band, and Slow-5 band.

Results

After the acupuncture treatments, the patients with PDM in the verum acupuncture group showed significantly decreased VAS scores (p < 0.05). The frequency-dependent gFCD alternations were found in the verum acupuncture group, altered regions including DLPFC, somatosensory cortex, anterior cingulate cortex (ACC), middle cingulate cortex (MCC), precuneus, hippocampus, and insula. The sham acupuncture modulated regions including angular gyrus, inferior frontal gyrus, and hippocampus. The gFCD alternation in DLPFC at the Slow-5 band was negatively in the patients with PDM following verum acupuncture, and S2 at the Slow-4 band was positively correlated with VAS scores.

Conclusion

These findings supported that verum acupuncture could effectively modulate frequency-dependent gFCD in PDM by influencing abnormal DLPFC at Slow-5 band and hippocampus at the Slow-3 band. The outcome of this study may shed light on enhancing the potency of acupuncture in clinical practice.
Preoperative Naples prognostic score is a reliable prognostic indicator for newly diagnosed glioblastoma patients
Authors: Li Junhong , Yang Wanchun , Yuan Yunbo , Zuo Mingrong , Li Tengfei , Wang Zhihao , Liu Yanhui
Published: August 2022

Background

Glioblastoma (GBM) accounts for approximately 80% of malignant gliomas and is characterized by considerable cellularity and mitotic activity, vascular proliferation, and necrosis. Naples prognostic score (NPS), based on inflammatory markers and nutritional status, has a prognostic ability in various cancers. In the current study, we aim to explore the prognostic value of operative NPS in GBM patients and compare the prognostic ability between NPS and controlling nutritional status (CONUT).

Materials and methods

The retrospective analysis was carried out on consecutive newly diagnosed GBM patients who had underwent tumor resection at West China Hospital from February 2016 to March 2019. All statistical analyses were conducted using SPSS software and R software.

Results

A total of 276 newly diagnosed GBM patients were enrolled in the current study. Overall survival (OS) (p < 0.001) and tumor location (p = 0.007) were significantly related to NPS. Serum albumin concentrate, cholesterol concentrate, neutrophil-to-lymphocyte ratio, lymphocyte ratio, and CONUT score were all significantly associated with NPS (p < 0.001). The Kaplan–Meier curve indicated that NPS (log-rank test, p < 0.001) and CONUT score (log-rank test, p = 0.023) were significantly associated with OS. Multivariate Cox regression revealed that both NPS and CONUT score served as independent prognostic indicators. The prognostic model with NPS had the strongest prognostic capability and best model-fitting.

Conclusion

In the current study, NPS is found as an independent prognostic indicator for patients with newly diagnosed GBM, and the prognostic ability of NPS is superior to CONUT score.
One-year recovery from breast cancer: Importance of tumor and treatment-related factors, resilience, and sociodemographic factors for health-related quality of life
Authors: Veličković Katarina , Borrebaeck Carl A. K. , Bendahl Pär-Ola , Hegardt Cecilia , Johnsson Per , Richter Corinna , Rydén Lisa , Hallberg Ingalill Rahm
Published: August 2022

Aim

This study investigated the changes in health-related quality of life from diagnosis to 1 year after diagnosis in breast cancer (BC) patients and the influence of clinical, psychological, and sociodemographic variables. An additional aim was to explore the mediating and moderating effects of resilience on changes in health-related quality of life.

Methods

A longitudinal population-based study was conducted in southern Sweden. Newly diagnosed BC patients filled in measures of health-related quality of life, resilience, and sociodemographic variables at diagnosis (N = 980) and 1 year post-diagnosis (N = 780). Clinical variables were extracted from the Swedish national breast cancer quality registry. Mixed-model analyses were performed.

Results

Most health-related quality of life outcomes declined from diagnosis to 1 year post-diagnosis. Role limitations due to emotional problems remained the same, whereas mental health improved. Lower health-related quality of life outcomes were associated with symptomatic detection and axillary dissection. Patients with a higher TNM stage and histologic grade and estrogen receptor (ER)-negative and human epidermal growth factor 2 (HER2)-positive status, who received chemotherapy, antibody therapy, or bisphosphonate therapy, had a steeper decline in outcomes. Changes in resilience were positively associated with all outcomes but did not mediate or moderate changes in any. Resilience at baseline moderated changes in bodily pain, vitality, and mental health, with higher baseline resilience being associated with a steeper decline, possibly due to floor or ceiling effects. Patients with lower socioeconomic status, educational level, and older age had a lower health-related quality of life.

Conclusion

Physical health-related quality of life among breast cancer patients declined 1 year post-diagnosis, whereas mental health-related quality of life improved. Low resilient patients may be especially vulnerable at diagnosis. Biopsychosocial assessment at diagnosis can help identify patients who may require additional support. A multidimensional treatment plan should be started early to help overcome the problems in everyday activities.
Efficacy of immune checkpoint inhibitors in non-small cell lung cancer: A systematic review and meta-analysis
Authors: Yang Fang , Wang Yucai , Tang Lin , Mansfield Aaron Scott , Adjei Alex A. , Leventakos Konstantinos , Duma Narjust , Wei Jia , Wang Lifeng , Liu Baorui , Molina Julian R.
Published: August 2022

Background

Immune checkpoint inhibitors (ICIs) have demonstrated remarkable efficacy in non-small cell lung cancer (NSCLC). However, only a minority of NSCLC patients benefit from ICIs, and whether the magnitude of benefit is specific factor-dependent remains unclear. We performed a systematic review to improve our understanding of clinicopathologic and biomolecular features associated with improved survival upon treatment with ICIs for NSCLC.

Methods

We searched PubMed, Web of Science, Embase, and Scopus from database inception to August 31, 2021, for randomized controlled trials (RCTs) comparing overall survival (OS) in NSCLC treated with ICIs vs control therapies. We calculated the pooled OS hazard ratio (HR) and 95% CI in subgroups using a random-effects model, and assessed the heterogeneity between the paired estimates using an interaction test.

Results

A total of 23 RCTs involving 15,829 patients were included. We found that wild-type EGFR, high PD-L1 expression, and high bTMB were associated with a significant OS benefit from ICIs, but not mutant EGFR, low PD-L1 expression, and low bTMB. The differences of OS benefit between wild-type and mutant EGFR (HR=1.53, 95%CI 1.13-2.08), high and low PD-L1 (HR=1.35; 95%CI 1.14-1.61), high and low bTMB (HR=1.71; 95%CI 1.17-2.52) were statistically significant. OS benefit was found in all subgroups regardless of sex, age, ECOG PS, histology, smoking history, baseline brain metastasis, race, and region, and the interaction test demonstrated no significant difference of the OS benefit between these opposed subgroups (e.g. male vs female).

Conclusions

Wild-type EGFR, high PD-L1 expression, and high bTMB are associated with a greater magnitude of efficacy from ICIs vs control therapies in NSCLC. However, the administration of ICIs should not be restricted to other clinicopathological factors (sex, smoking history, race, etc.).
Malignancy risk of gastrointestinal stromal tumors evaluated with noninvasive radiomics: A multi-center study
Authors: Wang Yun , Wang Yurui , Ren Jialiang , Jia Linyi , Ma Luyao , Yin Xiaoping , Yang Fei , Gao Bu-Lang
Published: August 2022

Purpose

This study was to investigate the diagnostic efficacy of radiomics models based on the enhanced CT images in differentiating the malignant risk of gastrointestinal stromal tumors (GIST) in comparison with the clinical indicators model and traditional CT diagnostic criteria.

Materials and methods

A total of 342 patients with GISTs confirmed histopathologically were enrolled from five medical centers. Data of patients wrom two centers comprised the training group (n=196), and data from the remaining three centers constituted the validation group (n=146). After CT image segmentation and feature extraction and selection, the arterial phase model and venous phase model were established. The maximum diameter of the tumor and internal necrosis were used to establish a clinical indicators model. The traditional CT diagnostic criteria were established for the classification of malignant potential of tumor. The performance of the four models was assessed using the receiver operating characteristics curve.

Reuslts

In the training group, the area under the curves(AUCs) of the arterial phase model, venous phase model, clinical indicators model, and traditional CT diagnostic criteria were 0.930 [95% confidence interval (CI): 0.895-0.965), 0.933 (95%CI 0.898-0.967), 0.917 (95%CI 0.872-0.961) and 0.782 (95%CI 0.717-0.848), respectively. In the validation group, the AUCs of the models were 0.960 (95%CI 0.930-0.990), 0.961 (95% CI 0.930-0.992), 0.922 (95%CI 0.884-0.960) and 0.768 (95%CI 0.692-0.844), respectively. No significant difference was detected in the AUC between the arterial phase model, venous phase model, and clinical indicators model by the DeLong test, whereas a significant difference was observed between the traditional CT diagnostic criteria and the other three models.

Conclusion

The radiomics model using the morphological features of GISTs play a significant role in tumor risk stratification and can provide a reference for clinical diagnosis and treatment plan.
Real-world effectiveness of Yindan Jiedu granules-based treatment on patients infected with the SARS-CoV-2 Omicron variants BA.2 combined with high-risk factors: A cohort study
Authors: Feng Ying , Liu Yao , Liu Long , Liu Yao , Jiang Yuyong , Hou Yixin , Zhou Yang , Song Rui , Chen Xiaoyou , Wang Xianbo
Published: August 2022

Background: Our previous studies have shown that Yindan Jiedu granules (YDJDG) can effectively treat coronavirus disease 2019 (COVID-19); however, the high infectivity and the immune escape potential of the Omicron variant BA.2 make it more difficult to control, and patients with high-risk factors prone to progress rapidly.

Purpose: To evaluate YDJDG’s efficacy in treating patients with the Omicron variant BA.2 with high-risk factors and compared it with that of Paxlovid.

Methods: A total of 257 patients who fulfilled the inclusion criteria were allocated to the YDJDG (115 cases), Paxlovid (115 cases), and control (27 cases) groups. A Cox regression model was used to analyze the independent factors affecting the shedding time of nucleic acid in 14 days. Propensity score matching (PSM) was used to match the characteristics of individuals in the three groups, while the Kaplan-Meier method was used to compare the shedding proportion of nucleic acids.

Results: Cox analysis showed that the vaccine booster (p = 0.006), YDJDG treatment (p = 0.020), and Paxlovid treatment (p < 0.0001) were independent predictors of nucleic acid shedding at 14 days. The median recovery time was 11.49 days in the YDJDG group, 10.21 days in the Paxlovid group, and 13.93 days in the control group. After PSM (3:1), the results showed that the nucleic acid shedding time of the YDJDG group (n = 53) was 2.47 days shorter than that of the control group (n = 21) (p = 0.0076), while the Paxlovid group (n = 44) had a 4.34 days shorter than that of the control group (n = 17) (p < 0.0001). After PSM (1:1), YDJDG and Paxlovid (76 pairs) were also analyzed. In the YDJDG group, nucleic acid shedding time was 1.43 days longer than that observed in the Paxlovid group (p = 0.020). At 10 and 14 days, the Paxlovid group showed a significant difference in the nucleic acid shedding proportion compared with the control group (p = 0.036, p = 0.0015). A significant difference was also observed between the YDJDG and control groups (p = 0.040) at 14 days.

Conclusion: As a safe and convenient oral drug, YDJDG can be used as an alternative to antiviral therapy for such patients.
QTL and candidate genes for heterophylly in soybean based on two populations of recombinant inbred lines
Authors: Chen Qiang , Liu Bingqiang , Ai Lijuan , Yan Long , Lin Jing , Shi Xiaolei , Zhao Hongtao , Wei Yu , Feng Yan , Liu Chunji , Yang Chunyan , Zhang Mengchen
Published: August 2022

Heterophylly, the existence of different leaf shapes and sizes on the same plant, has been observed in many flowering plant species. Yet, the genetic characteristics and genetic basis of heterophylly in soybean remain unknown. Here, two populations of recombinant inbred lines (RILs) with distinctly different leaf shapes were used to identify loci controlling heterophylly in two environments. The ratio of apical leaf shape (LSUP) to basal leaf shape (LSDOWN) at the reproductive growth stage (RLS) was used as a parameter for classifying heterophylly. A total of eight QTL were detected for RLS between the two populations and four of them were stably identified in both environments. Among them, qRLS20 had the largest effect in the JS population, with a maximum LOD value of 46.9 explaining up to 47.2% of phenotypic variance. This locus was located in the same genomic region as the basal leaf shape QTL qLSDOWN20 on chromosome 20. The locus qRLS19 had the largest effect in the JJ population, with a maximum LOD value of 15.2 explaining up to 27.0% of phenotypic variance. This locus was located in the same genomic region as the apical leaf shape QTL qLSUP19 on chromosome 19. Four candidate genes for heterophylly were identified based on sequence differences among the three parents of the two mapping populations, RT-qPCR analysis, and gene functional annotation analysis. The QTL and candidate genes detected in this study lay a foundation for further understanding the genetic mechanism of heterophylly and are invaluable in marker-assisted breeding.
Characterization of the gene expression profile response to drought stress in Haloxylon using PacBio single-molecule real-time and Illumina sequencing
Authors: Yang Fang , Lv Guanghui
Published: August 2022

Haloxylon ammodendron and Haloxylon persicum are important drought-tolerant plants in northwest China. The whole-genome sequencing of H. ammodendron and H. persicum grown in their natural environment is incomplete, and their transcriptional regulatory network in response to drought environment remains unclear. To reveal the transcriptional responses of H. ammodendron and H. persicum to an arid environment, we performed single-molecule real-time (SMRT) and Illumina RNA sequencing. In total, 20,246,576 and 908,053 subreads and 435,938 and 210,334 circular consensus sequencing (CCS) reads were identified by SMRT sequencing of H. ammodendron and H. persicum, and 15,238 and 10,135 unigenes, respectively, were successfully obtained. In addition, 9,794 and 7,330 simple sequence repeats (SSRs) and 838 and 71 long non-coding RNAs were identified. In an arid environment, the growth of H. ammodendron was restricted; plant height decreased significantly; basal and branch diameters became thinner and hydrogen peroxide (H2O2) content and peroxidase (POD) activity were increased. Under dry and wet conditions, 11,803 and 15,217 differentially expressed genes (DEGs) were identified in H. ammodendron and H. persicum, respectively. There were 319 and 415 DEGs in the signal transduction pathways related to drought stress signal perception and transmission, including the Ca2+ signal pathway, the ABA signal pathway, and the MAPK signal cascade. In addition, 217 transcription factors (TFs) and 398 TFs of H. ammodendron and H. persicum were differentially expressed, including FAR1, MYB, and AP2/ERF. Bioinformatic analysis showed that under drought stress, the expression patterns of genes related to active oxygen [reactive oxygen species (ROS)] scavenging, functional proteins, lignin biosynthesis, and glucose metabolism pathways were altered. Thisis the first full-length transcriptome report concerning the responses of H. ammodendron and H. persicum to drought stress. The results provide a foundation for further study of the adaptation to drought stress. The full-length transcriptome can be used in genetic engineering research.
How and when seeking feedback from coworkers pays off? The mixed role of coworker relationship
Authors: Zhang Wei , Qian Jing , Yu Haibo
Published: August 2022

Although research in the feedback-seeking behavior literature has primarily focused on feedback-seeking from supervisors, some emerging works have begun to explore the benefits of coworker feedback-seeking behavior. Based on the social exchange theory, we investigated how and when seeking feedback from coworkers will benefit the seekers. Using a sample of 327 teachers from China, we find that seeking feedback from coworkers is positively associated with task performance and workplace well-being. Seeking feedback from coworkers is also positively associated with coworker relationship. Moreover, the coworker relationship mediates the effects of seeking feedback from coworkers and task performance and workplace well-being, and moderates the strength of the relationship between seeking feedback from coworkers on task performance and workplace well-being. Theoretical and practical implications of our findings are discussed.
Using the gravitational mixed models to analyze the impact of China's foreign direct investment along with The Belt and Road countries on trade flows
Authors: Hsieh Te-Hsin , Zhu Ye-Bin , Huang Kuo-Lung
Published: August 2022

Since the “The Belt and Road” initiative was put forward in 2013, China's foreign investment growth rate has been greatly accelerated. In The Belt and Road context, many scholars used models to analyze the relationship between foreign direct investment, trade flows, and import and export trade. From literature reviews, it is found that previous scholars do not conform to reality and cannot be studied dynamically. Therefore, this study used the panel data of China's foreign direct investment and import and export trade in 40 countries along “The Belt and Road” from 2010 to 2019 to focuses on the impact of foreign direct investment (FDI) on trade flows, import trade and export trade. Regression analysis was carried out with the trade gravity model and Tinbergen's trade gravity model. In addition to model variables and arguments, the following control variables were adopted: exchange rate, natural resource rents, labor force population, differences in consumer ability, trade openness, and trade agreement signing. The results found that: (1) Foreign direct investment has a positive correlation with trade flow and import and export trade. (2) The labor force population has a negative correlation with trade flow, and import and export trade. (3) The expansion of China's economic scale can increase the scale of China's import and export trade, at the same time, the gap in consumption levels between the two countries will restrict the degree of import and export trade. (4) The possession of natural resources can also promote the development of trade.
Neuropsychiatric symptoms and altered sleep quality in cerebral small vessel disease
Authors: Li Xi , Qin Rong-Rong , Chen Jian , Jiang Hai-Fei , Tang Pan , Wang Yu-Jing , Xu Dong-Wu , Xu Tao , Yuan Ti-Fei
Published: August 2022

Background

Sleep disturbance and neuropsychiatric symptoms are common clinical symptoms of cerebral small vessel disease (CSVD), but the underlying mechanism is unclear. Here, we investigated the relationship between sleep quality and neuropsychiatric performance in patients with CSVD.

Methods

A total of 30 patients with CSVD and 35 healthy controls (HCs) were recruited. The 13-item Beck Depression Inventory (BDI-13), Beck Anxiety Inventory (BAI), and Symptom Check List 90 (SCL90) were used to assess depression, anxiety, and other psychological symptoms, respectively. Sleep quality was assessed using Pittsburgh Sleep Quality Index (PSQI), and cognitive function was tested using Montreal Cognitive Assessment (MoCA).

Results

When compared to the HC group, the patients with CSVD showed increased anxiety and neuropsychiatric symptoms, worse sleep quality, and impaired cognition (p < 0.05). The prevalence of comorbid poor sleep quality in the patients with CSVD was approximately 46%. The patients with CSVD with poor sleep quality also had more severe neuropsychiatric symptoms. After controlling for demographic variables, sex and anxiety significantly predicted sleep quality.

Conclusion

This study suggests that the prevalence of CSVD with poor sleep quality is high, and that sex and anxiety are independent risk factors for CSVD comorbid sleep quality.
A pan-cancer analysis of FAT atypical cadherin 4 (FAT4) in human tumors
Authors: Mao Weili , Zhou Jiajing , Hu Jie , Zhao Kui , Fu Zhenling , Wang Jun , Mao Kaili
Published: August 2022

Objective

We performed a pan-cancer analysis to explore the potential mechanisms of FAT4 in 33 different tumors.

Methods

In this study, we selected 33 types of cancers based on the datasets of TCGA (the cancer genome atlas). We analyzed the expression of FAT4 in tumor and normal tissues. Meanwhile, we analyzed the expression levels of FAT4 in tissues from tumors of different stages. Kaplan-Meier survival analysis, Tumor Mutational Burden (TMB), Microsatellite Instability (MSI), immune infiltration analysis, Gene set enrichment analysis (GSEA), and FAT4-related gene enrichment analysis were performed.

Results

FAT4 expression in most tumor tissues was lower than in corresponding control tissues. FAT4 expression was different in different stages of bladder cancer (BLCA), kidney clear cell carcinoma (KIRC), and breast cancer (BRCA). In addition, the expression level of FAT4 in different types of tumors has an important impact on the prognosis of patients. FAT4 might influence the efficacy of immunotherapy via tumor burden and microsatellite instability. We observed a statistically positive correlation between cancer-associated fibroblasts and FAT4 expression in most tumors. GSEA of BLCA indicated that low FAT4 expression groups were mainly enriched in calcium signaling pathway and chemokine signaling pathway. GSEA analysis of KIRC suggested low FAT4 expression groups were mainly involved in olfactory transduction and oxidative phosphorylation. Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the role of FAT4 in the pathogenesis of cancer may be related to human papillomavirus infection, Hippo signaling pathway, PI3K–Akt signaling pathway, etc. Gene Ontology (GO) enrichment analysis further showed that most of these genes were related to the pathways or cell biology, such as peptidyl–tyrosine phosphorylation, cell junction assembly, protein tyrosine kinase activity, etc.

Conclusion

Our study summarized and analyzed the antitumor effect of FAT4 in different tumors comprehensively, which aided in understanding the role of FAT4 in tumorigenesis from the perspective of clinical tumor samples. Pan-cancer analysis showed that FAT4 to be novel biomarkers for various cancers prognosis.
Sensitizing the social-ecosystems of outdoor sport environments: A comprehensive framework
Authors: Trendafilova Sylvia , Ziakas Vassilios
Published: August 2022

This paper focuses on the social features of participation in outdoor sports that play a significant role in the lived experience of participants, and in their interactions with the environment. These embodied interactions can bridge nature and culture, and inform interventions for more sustainable ecosystems. Conceptual methods were used to explain the sport-nature-culture nexus and postulate an interdisciplinary framework of social sport ecology, incorporating management, nature sports, neo-tribalism, and non-representation theoretical perspectives. The proposed framework suggests that multi-sensory stimuli, embodied sport practices and neo-tribal cultural values shape the “sports ecosphere,” which needs to be attuned with the affective/cognitive dimensions of experience in ways that build caring cultures for the environment. The significance of this work lies in its comprehensive perspective to the environmental management of outdoor sports by demonstrating the critical role of politics, culture, experience and movement in contemporary sport. It suggests a holistic approach of social sport ecology to better understand and reimagine the environmental practices and character of outdoor sports.
Developing a Moving Average Crossover Strategy as an Alternative Hedging Strategy for the South Africa Maize Market
Authors: Monteiro Markus Arlindo , Grové Bennie , Matthews Nicolette
Published: August 2022

Grain marketing is complex because important decisions are made on the timing of sales and the quantities sold at every trading activity. The literature suggest various grain-hedging strategies, however these strategies are not adaptable to changing market conditions or are difficult for a producer to implement. To address these limitations, our study developed tailor-made moving average crossover (MAC) strategies that are adaptable to changing market conditions and can be easily followed by risk neutral and risk averse grain producers. The study used daily closing prices for the white maize May futures contract for the period 2009/2010 to 2019/2020. An optimization model was solved using the evolutionary algorithm embedded in Excel® to identify the optimal MAC strategy that maximizes the margin above marketing cost for a risk aversion level. The results showed that optimal MAC strategies differ amongst producers with different levels of risk aversion. Furthermore, it was found that the risk-averse producers perform best by marketing their grain early in the marketing season. Meanwhile, the risk-neutral producers perform better by spreading their marketing activities throughout the season. The results further showed that the optimal MAC strategies performed better than the previously proposed routine strategies. The conclusion is therefore that an optimal MAC strategy outperforms routine strategies because of its ability to adapt to changing market conditions, while still being easy to implement.
Long-Term Field Biochar Application for Rice Production: Effects on Soil Nutrient Supply, Carbon Sequestration, Crop Yield and Grain Minerals
Authors: Xu Qiao , Wang Ji , Liu Qi , Chen Zhe , Jin Penghui , Du Jiazhou , Fan Jialu , Yin Weiqin , Xie Zubin , Wang Xiaozhi
Published: August 2022

Research is still under-represented for the long-term impacts of field biochar application on soil fertility, rice production and mineral nutrition and soil C sequestration. To investigate these effects, a field trial was established in the fall of 2010 with low (2.4 t ha−1), intermediate (6 t ha−1) and high (12 t ha−1) biochar doses. The biochar effect on soil nutrients is more pronounced in the surface soil and is dose dependent. In the surface soil, biochar application increased the availability of soil N, P and K. High dose decreased soil total Fe by 11.6% and Mn by 20.7%, leading to a slight but insignificant decrease in grain Fe and Mn concentration. The intermediate and high doses significantly increased the soil available Zn by 96.2% and 227.9% but did not affect the grain Zn concentration. Grain Cu concentration was significantly decreased by the intermediate (−19.6%) and high (−14.3%) dose of biochar. Biochar had a slight but insignificant boost on rice yield. Soil total C and N increased linearly with biochar application for the surface soil only where biochar was applied. In conclusion, with great soil C sequestration capacity, the long-term field return of biochar is beneficial for improving the soil macronutrient and Zn fertility, but caution is needed, as higher doses of biochar could potentially lead to a decrease in soil Fe and Mn and a reduction in grain Cu concentration.
Polyphenols from Thinned Young Apples: HPLC-HRMS Profile and Evaluation of Their Anti-Oxidant and Anti-Inflammatory Activities by Proteomic Studies
Authors: Ferrario Giulio , Baron Giovanna , Gado Francesca , Della Vedova Larissa , Bombardelli Ezio , Carini Marina , D’Amato Alfonsina , Aldini Giancarlo , Altomare Alessandra
Published: August 2022

The qualitative profile of thinned apple polyphenols (TAP) fraction (≈24% of polyphenols) obtained by purification through absorbent resin was fully investigated by LC-HRMS in positive and negative ion mode and using ESI source. A total of 68 polyphenols were identified belonging to six different classes: flavanols, flavonols, dihydrochalchones, flavanones, flavones and organic and phenolic acids. The antioxidant and anti-inflammatory activities were then investigated in cell models with gene reporter for NRF2 and NF-κB and by quantitative proteomic (label-free and SILAC) approaches. TAP dose-dependently activated NRF2 and in the same concentration range (10–250 µg/mL) inhibited NF-κB nuclear translocation induced by TNF-α and IL-1α as pro-inflammatory promoters. Proteomic studies elucidated the molecular pathways evoked by TAP treatment: activation of the NRF2 signaling pathway, which in turn up-regulates protective oxidoreductases and their nucleophilic substrates such as GSH and NADPH, the latter resulting from the up-regulation of the pentose phosphate pathway. The increase in the enzymatic antioxidant cellular activity together with the up-regulation of the heme-oxygenase would explain the anti-inflammatory effect of TAP. The results suggest that thinned apples can be considered as a valuable source of apple polyphenols to be used in health care products to prevent/treat oxidative and inflammatory chronic conditions.
Study on a Transparent Similar Rock-Anchoring Structure under Impact Tests and Numerical Simulation Tests
Authors: Zhu Changxing , Liu Xu , An Yeming , Zhao Weihao
Published: August 2022

Interface slip is one of the main failure forms of an anchor structure, but the debonding slip process of rockbolt cannot be directly observed under impact load, so it is important to study the failure mechanism of the interface debonding of an anchor structure. For this purpose, a kind of transparent similar rock-anchoring structure and a new rockbolt impact load test system were developed. The debonding process of the anchor structure was carried out based on the system and Particle Flow Code (PFC) simulation test. The main conclusions are as follows: Axial force of rockbolt decreases progressively from the load end to the other end, but the shear stress of the interfaces increases at the early stage and then decreases. The majority of shear stress is at I interface compared to shear stress at II interface. The crack in the transparent sample occurs first in the middle of the sample, then extends to the local position of the two interfaces and finally penetrates all cracks, and the debonding failure mode is dual-interface mixed. According to laboratory tests and PFC simulation tests, the crack growth process can be divided into three stages. The results of the PFC simulation tests are similar to those of the laboratory impact tests of rockbolt.