bluuebunny/crossref_metadata_2025 huggingface dataset:
The Antimicrobial Potential of Bacteria Isolated from Honey Samples Produced in the Apiaries Located in Pomeranian Voivodeship in Northern Poland
Authors: Pajor Magdalena , Worobo Randy W. , Milewski Sławomir , Szweda Piotr
Published: September 2018

The principal objective of this study was to determine whether the honeys produced in apiaries located in Pomeranian Voivodeship (Northern Poland) contain bacteria producing metabolites with growth inhibition potential against important human and animal pathogens. The pathogens included Staphylococcus aurues, Staphyloccocus epidermidis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Candida albicans. From 12 samples of honey, 163 strains of bacteria were isolated. Activity against reference staphylococci: S. aurues ATCC 25923; S. aureus ATCC 29213; S. epidermidis 12228 was observed in 33 (20.3%), 38 (23.3%), and 41 (25.1%) isolates, respectively. High inhibitory activity was also found against Listeria monocytogenes ATCC 7644 in 34 strains (20.9%). Activity against Candida albicans ATCC 10231 and especially Gram-negative bacteria: Pseudomonas aeruginosa ATCC 27857 and Escherichia coli ATCC 25922 was rarely observed. Production of metabolites exhibiting activity against the three pathogens mentioned above was confirmed for 13 (7.8%), 3 (1.8%), and 2 (1.2%) isolates, respectively. Forty-six isolates were selected for further analysis. Within this group, metabolites synthesized by 18 producing strains (39.13%) inhibited growth of only one of the reference strains of pathogenic microorganisms. However, 14 (30.44%), 8 (17.39%), and 6 (13.04%) strains produced agents active against three, two, and four pathogens, respectively. Sequencing of the 16S rRNA gene revealed that 80.4% of these 46 producing strains belong to the genus Bacillus. However, some producing strains belonging to the genus of Peanibacillus, Lysinibacillus, Microbacterium, and Staphylococcus were also identified. Furthermore, the analysis of the sequences of 16S rRNA, as well as RAPD-PCR, exhibited a significant diversity in the strains tested, even in the case of bacteria isolated from the same honey (and classified to the same genus, usually Bacillus spp.). This observation suggests environmental origin (nectar, water, or pollen) of the producing strains. The research carried out confirmed that honey produced in Northern Poland is a promising source of strains of bacteria producing metabolites with antimicrobial activity.
Treatment of Sewage Using a Constructed Soil Rapid Infiltration System Combined with Pre-Denitrification
Authors: Xu Wenlai , Chen Jinyao , Jian Yue , Pan Zhicheng , Mou Zishen
Published: September 2018

The activated sludge process of the anaerobic/oxic (A/O) process has a good denitrification performance because it can make full use of the carbon source in the original sewage, and the denitrification can provide alkalinity for aerobic nitrification. The traditional constructed soil rapid infiltration (CSRI) system, on the other hand, has a poor nitrogen removal effect. Dividing the traditional CSRI system into two sections, one performs denitrification as an anoxic section, while the other performs nitrification as an aerobic section and is placed after the anoxic section. The nitrification liquid of the effluent from the aerobic section is mixed with the original wastewater and enters the anoxic section for denitrification. We expected that this would be improved by combining CSRI with a pre-denitrification step that would make full use of the carbon source in the original sewage. In a small-scale experimental model, the removal efficiencies of nitrogen, in the form of ammonium, nitrate, and total nitrogen (TN), as well as chemical oxygen demand (COD), were determined. The hydraulic load was varied, while the backflow reflux capacity was kept constant, to determine the effect on the pre-denitrification process. An average removal rate of 95.4% for NH4+-N and 96% for COD could be obtained when a hydraulic load of 80 cm3(cm2·d)−1 and a reflux ratio of 75% were applied. Under these conditions, the average removal rate of TN was 77.4%, which is much higher than what can be typically achieved with conventional CSRI systems.
Surveying Alcohol Outlet Density in Four Neighborhoods of Beirut Lebanon: Implications for Future Research and National Policy
Authors: Nakkash Rima , Ghandour Lilian A. , Anouti Sirine , Nicolas Jessika , Chalak Ali , Yassin Nasser , Afifi Rima
Published: September 2018

Underage drinking among youth in Lebanon is increasing. Regulating availability is one of the best buy policies recommended by the World Health Organization. To quantitatively document the current status of alcohol availability to youth in Lebanon, we used GPS technology to survey alcohol outlet density in four highly populated neighborhoods in Beirut, Lebanon, and to estimate their proximity to educational institutions. The density of alcohol outlets ranged from 18.30 to 80.95 per km2 (average of 39.6 alcohol outlets/km2). The highest number of total alcohol outlets was in the “Hamra & Jamiaa” area, which includes one of the largest private universities in the country. Thirteen out of 109 (12%) alcohol outlets (on and off-premise) were located less 100 m away from educational institutions, in violation of the current licensing law. None of the off-premise and the majority (94%) of on-premise alcohol outlets displayed the “no sale for <18” sign. Findings were indicative of an environment conducive to increased access and availability of alcohol among youth in Lebanon probably attributed to the prevailing weak alcohol policies and their enforcement. Systematic collection and reporting of alcohol outlet densities is critical to understand the alcogenic environment and guide local harm reduction policies.
Diagnosis on Transport Risk Based on a Combined Assessment of Road Accidents and Watershed Vulnerability to Spills of Hazardous Substances
Authors: Machado Emerson Ribeiro , Valle Junior Renato Farias do , Pissarra Teresa Cristina Tarlé , Siqueira Hygor Evangelista , Sanches Fernandes Luís Filipe , Pacheco Fernando António Leal
Published: September 2018

Roads play an important role in the economic development of cities and regions, but the transport of cargo along highways may represent a serious environmental problem because a large portion of transported goods is composed of dangerous products. In this context, the development and validation of risk management tools becomes extremely important to support the decision-making of people and agencies responsible for the management of road enterprises. In the present study, a method for determination of environmental vulnerability to road spills of hazardous substances is coupled with accident occurrence data in a highway, with the purpose to achieve a diagnosis on soil and water contamination risk and propose prevention measures and emergency alerts. The data on accident occurrences involving hazardous and potentially harmful products refer to the highway BR 050, namely the segment between the Brazilian municipalities of Uberaba and Uberlândia. The results show that many accidents occurred where vulnerability is high, especially in the southern sector of the segment, justifying the implementation of prevention and alert systems. The coupling of vulnerability and road accident data in a geographic information system proved efficient in the preparation of quick risk management maps, which are essential for alert systems and immediate environmental protection. Overall, the present study contributes with an example on how the management of risk can be conducted in practice when the transport of dangerous substances along roads is the focus problem.
Dental-Dam for Infection Control and Patient Safety during Clinical Endodontic Treatment: Preferences of Dental Patients
Authors: Madarati Ahmad , Abid Seema , Tamimi Faisal , Ezzi Ali , Sammani Aya , Shaar Mohamad Bachar Abou Al , Zafar Muhammad
Published: September 2018

Background: A number of factors (first experience, treating clinician and time to place dental-dam) may influence patients’ preferences regarding dental-dams. In general, patients accept placing it and that it must be used for teeth isolation during endodontic procures for the sake of patient safety and infection control. Objectives: The aim of this study is to investigate preferences and experiences of patients using dental-dam (DD) isolation during root canal treatment (RCT) and to explore influencing factors among the residents of Madinah Munnawara, Saudi Arabia. Methods: Following an ethical approval and a pilot study, a self-administrated questionnaire was distributed to 305 patients attending endodontic clinics at the Taibah University College of Dentistry (TUCOD) over six months. Patients voluntarily participated in the study after understanding the methodologies and signing a consent form. They were asked to fill out a questionnaire on their experiences and preferences in placing the DD during RCT. Data were analyzed using the Chi-square test at p = 0.05. Results: The response rate was 91%. There was no significant correlation between patients’ preferences and their race, age and gender (p > 0.05). The majority of participants (74.3%) would prefer to use a DD in their next session (p < 0.001). This preference negatively correlated with the time required to place a DD and the duration of the current visit (p < 0.001). While most of those who would prefer to use a DD in their next visit were pleased with how it was placed in the current session (76.6%), most of those who would not do so (66.7%) were uncomfortable. Overall, the highest proportion of participants (40.2%) reported that prevention of instrument swallowing was the most important advantage of DD isolation (p < 0.001). Conclusions: Overall, DD isolation for RCT is generally well accepted by patients regardless of their country of origin, gender, education and awareness of its advantages. Patients’ safety was the most attractive advantage for patients to the application of the DD. The time required to place the dental dam and first visit experience in placing the DD affect patients’ future preference.
Phosphatidylethanolamine Induces an Antifibrotic Phenotype in Normal Human Lung Fibroblasts and Ameliorates Bleomycin-Induced Lung Fibrosis in Mice
Authors: Vazquez-de-Lara Luis G. , Tlatelpa-Romero Beatriz , Romero Yair , Fernández-Tamayo Nora , Vazquez-de-Lara Fernando , M. Justo-Janeiro Jaime , Garcia-Carrasco Mario , De-la-Rosa Paredes René , Cisneros-Lira José G. , Mendoza-Milla Criselda , Moccia Francesco , Berra-Romani Roberto
Published: September 2018

Lung surfactant is a complex mixture of phospholipids and specific proteins but its role in the pathogenesis of interstitial lung diseases is not established. Herein, we analyzed the effects of three representative phospholipid components, that is, dipalmitoilphosphatidylcoline (DPPC), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE), on collagen expression, apoptosis and Ca2+ signaling in normal human lung fibroblasts (NHLF) and probed their effect in an experimental model of lung fibrosis. Collagen expression was measured with RT-PCR, apoptosis was measured by using either the APOPercentage assay kit (Biocolor Ltd., Northern Ireland, UK) or the Caspase-Glo 3/7 assay (Promega, Madison, WI, USA) and Ca2+ signaling by conventional epifluorescence imaging. The effect in vivo was tested in bleomycin-induced lung fibrosis in mice. DPPC and PG did not affect collagen expression, which was downregulated by PE. Furthermore, PE promoted apoptosis and induced a dose-dependent Ca2+ signal. PE-induced Ca2+ signal and apoptosis were both blocked by phospholipase C, endoplasmic reticulum pump and store-operated Ca2+ entry inhibition. PE-induced decrease in collagen expression was attenuated by blocking phospholipase C. Finally, surfactant enriched with PE and PE itself attenuated bleomycin-induced lung fibrosis and decreased the soluble collagen concentration in mice lungs. This study demonstrates that PE strongly contributes to the surfactant-induced inhibition of collagen expression in NHLF through a Ca2+ signal and that early administration of Beractant enriched with PE diminishes lung fibrosis in vivo.
The PIN-FORMED Auxin Efflux Carriers in Plants
Authors: Zhou Jing-Jing , Luo Jie
Published: September 2018

Auxin plays crucial roles in multiple developmental processes, such as embryogenesis, organogenesis, cell determination and division, as well as tropic responses. These processes are finely coordinated by the auxin, which requires the polar distribution of auxin within tissues and cells. The intercellular directionality of auxin flow is closely related to the asymmetric subcellular location of PIN-FORMED (PIN) auxin efflux transporters. All PIN proteins have a conserved structure with a central hydrophilic loop domain, which harbors several phosphosites targeted by a set of protein kinases. The activities of PIN proteins are finely regulated by diverse endogenous and exogenous stimuli at multiple layers—including transcriptional and epigenetic levels, post-transcriptional modifications, subcellular trafficking, as well as PINs’ recycling and turnover—to facilitate the developmental processes in an auxin gradient-dependent manner. Here, the recent advances in the structure, evolution, regulation and functions of PIN proteins in plants will be discussed. The information provided by this review will shed new light on the asymmetric auxin-distribution-dependent development processes mediated by PIN transporters in plants.
Severity of Sjögren’s Syndrome Keratoconjunctivitis Sicca Increases with Increased Percentage of Conjunctival Antigen-Presenting Cells
Authors: Pflugfelder Stephen C. , Bian Fang , Gumus Koray , Farley William , Stern Michael E. , De Paiva Cintia S.
Published: September 2018

This study investigated the relationship between clinical severity and percentage of conjunctival antigen-presenting cells (APCs) in Sjögren’s syndrome (SS)-associated keratoconjunctivitis sicca (KCS). KCS clinical severity was based on symptom severity, tear volume, tear break-up time, and ocular surface dye staining. Conjunctival goblet cell density (GCD) was measured in periodic acid Schiff (PAS)-stained membranes. Conjunctival cells obtained by impression cytology were used for flow cytometry to measure percentages of CD45+HLA-DR+ APCs and mature CD11c+CD86+ dendritic cells (DCs). Compared to normal conjunctiva, the percentages of HLA-DR+ and CD11c+CD86+ cells were higher in the conjunctiva of the KCS group (p < 0.05). The percentage of CD45+HLA-DR+ cells positively correlated with clinical severity (r = 0.71, p < 0.05) and negatively correlated with GCD (r = −0.61, p < 0.05). Clinical severity also negatively correlated with GCD (r = −0.54, p < 0.05). These findings indicate that a higher percentage of APCs and mature DCs in the conjunctiva is associated with more severe KCS in SS. These APCs may contribute to the generation of the pathogenic Th1 cells that cause goblet cell loss in KCS.
Structural Features of Amyloid Fibrils Formed from the Full-Length and Truncated Forms of Beta-2-Microglobulin Probed by Fluorescent Dye Thioflavin T
Authors: Sulatskaya Anna I. , Rodina Natalia P. , Polyakov Dmitry S. , Sulatsky Maksim I. , Artamonova Tatyana O. , Khodorkovskii Mikhail A. , Shavlovsky Mikhail M. , Kuznetsova Irina M. , Turoverov Konstantin K.
Published: September 2018

The persistence of high concentrations of beta-2-microglobulin (β2M) in the blood of patients with acute renal failure leads to the development of the dialysis-related amyloidosis. This disease manifests in the deposition of amyloid fibrils formed from the various forms of β2M in the tissues and biological fluids of patients. In this paper, the amyloid fibrils formed from the full-length β2M (β2m) and its variants that lack the 6 and 10 N-terminal amino acids of the protein polypeptide chain (ΔN6β2m and ΔN10β2m, respectively) were probed by using the fluorescent dye thioflavin T (ThT). For this aim, the tested solutions were prepared via the equilibrium microdialysis approach. Spectroscopic analysis of the obtained samples allowed us to detect one binding mode (type) of ThT interaction with all the studied variants of β2M amyloid fibrils with affinity ~104 M−1. This interaction can be explained by the dye molecules incorporation into the grooves that were formed by the amino acids side chains of amyloid protofibrils along the long axis of the fibrils. The decrease in the affinity and stoichiometry of the dye interaction with β2M fibrils, as well as in the fluorescence quantum yield and lifetime of the bound dye upon the shortening of the protein amino acid sequence were shown. The observed differences in the ThT-β2M fibrils binding parameters and characteristics of the bound dye allowed to prove not only the difference of the ΔN10β2m fibrils from other β2M fibrils (that can be detected visually, for example, by transmission electron microscopy (TEM), but also the differences between β2m and ΔN6β2m fibrils (that can not be unequivocally confirmed by other approaches). These results prove an essential role of N-terminal amino acids of the protein in the formation of the β2M amyloid fibrils. Information about amyloidogenic protein sequences can be claimed in the development of ways to inhibit β2M fibrillogenesis for the treatment of dialysis-related amyloidosis.
Comparison of Antibacterial Adhesion When Salivary Pellicle Is Coated on Both Poly(2-hydroxyethyl-methacrylate)- and Polyethylene-glycol-methacrylate-grafted Poly(methyl methacrylate)
Authors: Lee Bor-Shiunn , Chen Yu-Jia , Wei Ta-Chin , Ma Tien-Li , Chang Che-Chen
Published: September 2018

Although poly(2-hydroxyethyl methacrylate) (pHEMA) and polyethylene glycol methacrylate (PEGMA) have been demonstrated to inhibit bacterial adhesion, no study has compared antibacterial adhesion when salivary pellicle is coated on polymethyl methacrylate (PMMA) grafted with pHEMA and on PMMA grafted with PEGMA. In this study, PMMA discs were fabricated from a commercial orthodontic acrylic resin system (Ortho-Jet). Attenuated total reflection-Fourier transform infrared spectra taken before and after grafting confirmed that pHEMA and PEGMA were successfully grafted on PMMA. Contact angle measurements revealed PMMA-pHEMA to be the most hydrophilic, followed by PMMA-PEGMA, and then by PMMA. Zeta potential analysis revealed the most negative surface charges on PMMA-PEGMA, followed by PMMA-pHEMA, and then by PMMA. Confocal laser scanning microscopy showed green fluorescence in the background, indicating images that influenced the accuracy of the quantification of live bacteria. Both the optical density value measured at 600 nm and single plate-serial dilution spotting showed that pHEMA was more effective than PEGMA against Escherichia coli and Streptococcus mutans, although the difference was not significant. Therefore, the grafting of pHEMA and PEGMA separately on PMMA is effective against bacterial adhesion, even after the grafted PMMA were coated with salivary pellicle. Surface hydrophilicity, bactericidality, and Coulomb repulsion between the negatively charged bacteria and the grafted surface contributed to the effectiveness.
Proteasome-Rich PaCS as an Oncofetal UPS Structure Handling Cytosolic Polyubiquitinated Proteins. In Vivo Occurrence, in Vitro Induction, and Biological Role
Authors: Solcia Enrico , Necchi Vittorio , Sommi Patrizia , Ricci Vittorio
Published: September 2018

In this article, we outline and discuss available information on the cellular site and mechanism of proteasome interaction with cytosolic polyubiquitinated proteins and heat-shock molecules. The particulate cytoplasmic structure (PaCS) formed by barrel-like particles, closely reproducing in vivo the high-resolution structure of 26S proteasome as isolated in vitro, has been detected in a variety of fetal and neoplastic cells, from living tissue or cultured cell lines. Specific trophic factors and interleukins were found to induce PaCS during in vitro differentiation of dendritic, natural killer (NK), or megakaryoblastic cells, apparently through activation of the MAPK-ERK pathway. Direct interaction of CagA bacterial oncoprotein with proteasome was shown inside the PaCSs of a Helicobacter pylori-infected gastric epithelium, a finding suggesting a role for PaCS in CagA-mediated gastric carcinogenesis. PaCS dissolution and autophagy were seen after withdrawal of inducing factors. PaCS-filled cell blebs and ectosomes were found in some cells and may represent a potential intercellular discharge and transport system of polyubiquitinated antigenic proteins. PaCS differs substantially from the inclusion bodies, sequestosomes, and aggresomes reported in proteinopathies like Huntington or Parkinson diseases, which usually lack PaCS. The latter seems more linked to conditions of increased cell proliferation/differentiation, implying an increased functional demand to the ubiquitin–proteasome system.
Drug Repurposing of Metabolic Agents in Malignant Glioma
Authors: Seliger Corinna , Hau Peter
Published: September 2018

Gliomas are highly invasive brain tumors with short patient survival. One major pathogenic factor is aberrant tumor metabolism, which may be targeted with different specific and unspecific agents. Drug repurposing is of increasing interest in glioma research. Drugs interfering with the patient’s metabolism may also influence glioma metabolism. In this review, we outline definitions and methods for drug repurposing. Furthermore, we give insights into important candidates for a metabolic drug repurposing, namely metformin, statins, non-steroidal anti-inflammatory drugs, disulfiram and lonidamine. Advantages and pitfalls of drug repurposing will finally be discussed.
Cytokine-Modulated Natural Killer Cells Differentially Regulate the Activity of the Hepatitis C Virus
Authors: Cho Yoo , Lee Hwan , Kang Hyojeung , Cho Hyosun
Published: September 2018

HCV genotype 2a strain JFH-1 replicates and produces viral particles efficiently in human hepatocellular carcinoma (huh) 7.5 cells, which provide a stable in vitro cell infection system for the hepatitis C virus (HCVcc system). Natural killer (NK) cells are large lymphoid cells that recognize and kill virus-infected cells. In this study, we investigated the interaction between NK cells and the HCVcc system. IL-10 is a typical immune regulatory cytokine that is produced mostly by NK cells and macrophages. IL-21 is one of the main cytokines that stimulate the activation of NK cells. First, we used anti-IL-10 to neutralize IL-10 in a coculture of NK cells and HCVcc. Anti-IL-10 treatment increased the maturation of NK cells by enhancing the frequency of the CD56+dim population in NK-92 cells. However, with anti-IL-10 treatment of NK cells in coculture with J6/JFH-1-huh 7.5 cells, there was a significant decrease in the expression of STAT1 and STAT5 proteins in NK-92 cells and an increase in the HCV Core and NS3 proteins. In addition, rIL-21 treatment increased the frequency of the CD56+dim population in NK-92 cells, Also, there was a dramatic increase in the expression of STAT1 and STAT5 proteins in rIL-21 pre-stimulated NK cells and a decrease in the expression of HCV Core protein in coculture with J6/JFH-1-huh 7.5 cells. In summary, we found that the functional activation of NK cells can be modulated by anti-IL-10 or rIL-21, which controls the expression of HCV proteins as well as HCV RNA replication.
Combination of Diagnostic Tools for the Proper Identification of Moisture Pathologies in Modern Residential Buildings
Authors: Hidalgo-Betanzos Juan María , Escudero-Revilla César , Iribar-Solaberrieta Eider , Flores-Abascal Iván , Sala-Lizarraga José María Pedro
Published: September 2018

A study of moisture pathologies in a modern residential multifamily building is presented. The housing block was designed under the regulation NBE-CT of 1979 in northern Spain. After the appearance of some moisture problems in the façades, three complementary studies were conducted to analyze the situation of the envelope and diagnose the best improvement possibilities. First, indoor conditions of temperature and humidity of the apartments with moisture pathologies were monitored. During 40 winter days, the occupancy, heating operation, and natural ventilation were analyzed. Second, the inner and outer surface temperatures of the studied façades were measured. Thermal insulation degree, thermal capacity, and thermal bridge effects were measured to assess the risk of interstitial condensation under the real conditions of use. Third, an infrared thermographic survey was carried out, which allowed the detection of irregularities and the assessment of moisture problems. The wrong interpretations, which would have been made if the complementary studies had not been done, are exposed. The key towards the accurate diagnosis was the combination of tools. Finally, some technical solutions based on ventilation or thermal insulation enhancement are proposed as different ways to reduce the high levels of relative humidity indoors and minimize the risk of condensation in the future.
Detection of Volatile Constituents from Food Lures by Tephritid Fruit Flies
Authors: Biasazin Tibebe Dejene , Chernet Haimanot Teklemariam , Herrera Sebastian Larsson , Bengtsson Marie , Karlsson Miriam Frida , Lemmen-Lechelt Joelle Kristin , Dekker Teun
Published: September 2018

Tephritid fruit flies require protein for sexual and gonotrophic development. Food-based lures are therefore widely used in strategies to detect and control fruit flies in the Tephritidae family. However, these baits are attractive to a broad range of insect species. We therefore sought to identify volatiles detected by the fly antennae, with the goal to compose lures that more specifically target tephritids. Using gas chromatography-coupled electroantennographic detection (GC-EAD) we screened for antennal responses of four important tephritid species to volatile compounds from five commercially available protein-based baits. Antennal active compounds were reconstituted in synthetic blends for each species and used in behavioral assays. These species-based blends were attractive in olfactometer experiments, as was a blend composed of all antennally active compounds from all the four species we observed (tested only in Bactrocera dorsalis, Hendel). Pilot field tests indicate that the blends need to be further evaluated and optimized under field conditions.
Automated Purification of Radiometals Produced by Liquid Targets
Authors: Alves Vítor H. , Do Carmo Sérgio J. C. , Alves Francisco , Abrunhosa Antero J.
Published: September 2018

An automated process for the production and purification of radiometals produced by irradiating liquid targets in a medical cyclotron, using a commercially available module, has been developed. The method is suitable for the production and purification of radiometals such as 68Ga, 64Cu and 61Cu through irradiation of liquid targets and is important for producing high specific activity radioisotopes with a substantial reduction in processing time and cost when compared with the solid target approach. The “liquid target” process also eliminates the need for pre- and post-irradiation target preparation and simplifies the transfer of irradiated material from target to hotcell. A 68GaCl3 solution can be obtained in about 35 min with an average yield of 73.9 ± 6.7% in less than 10 mL of volume. 64CuCl2 solutions can be obtained with an average yield of 81.2 ± 7.8% in about 1 h of processing time. A dedicated single-use disposable kit is used on a commercial IBA Synthera® extension module.
Individualized Transcriptional Resolution of Complicated Malaria in a Colombian Study
Authors: Rojas-Peña Mónica , Duan Meixue , Arafat Dalia , Rengifo Lina , Herrera Socrates , Arévalo-Herrera Myriam , Gibson Greg
Published: September 2018

To evaluate whether recovery from complicated malaria follows a common trajectory in terms of immunological mechanism or, rather, is highly individualized for each patient, we performed longitudinal gene expression profiling of whole blood. RNA sequencing (RNAseq) was performed on blood samples obtained from eight patients on four consecutive days between hospital admission and discharge. Six patients were infected with Plasmodium falciparum, and two with Plasmodium vivax; one patient was a pregnant woman infected with P. falciparum, who was hospitalized for several weeks. The characterization of blood transcript modules (BTM) and blood informative transcripts (BIT) revealed that patients’ responses showed little commonality, being dominated by the balance of gene activity relating to lymphocyte function, inflammation, and interferon responses specific to each patient. Only weak correlations with specific complicated malaria symptoms such as jaundice, thrombocytopenia, or anemia were observed. The differential expression of individual genes, including transcripts derived from the human leukocyte antigen (HLA) complex, generally reflected differences in the underlying immune processes. Although the results of this pilot study do not point to any single process that might provide a target for complicated malaria treatment or prevention or personalized medical strategies, larger patient series and more extensive blood sampling may allow the classification of patients according to their type of response in order to develop novel therapeutic approaches.
Investigation of Energy Transfer in Star-Shaped White Polymer Light-Emitting Devices via the Time-Resolved Photoluminescence
Authors: He Hui , Liao Xiaoqing , Cheng Jiang , Li Ying , Yu Junsheng , Li Lu
Published: September 2018

A series of white polymer light-emitting devices (WPLEDs) were fabricated by utilizing star-shaped white-emission copolymers containing tri[1-phenylisoquinolinato-C2,N]iridium (Ir(piq)3), fluorenone (FO) and poly(9,9-dioctylfluorene) (PFO) as red-, green- and blue-emitting (RGB) components, respectively. In these WPLEDs, a maximum current efficiency of 6.4 cd·A−1 at 20 mA·cm−2 and Commission Internationale d’Eclairage (CIE) coordinates of (0.33, 0.32) were achieved, and the current efficiency was still kept to 4.2 cd·A−1 at the current density of 200 mA·cm−2. To investigate energy transfer processes among the three different chromophores of the star-shaped copolymers in these WPLEDs, the time-resolved photoluminescence (PL) spectra were recorded. By comparing the fluorescence decay lifetimes of PFO chromophores in the four star-like white-emitting copolymers, the efficient energy transfer from PFO units to Ir(piq)3 and FO chromophores was confirmed. From time-resolved PL and the analysis of energy transfer process, the results as follows were proved. Owing to the star-like molecular structure and steric hindrance effect, intermolecular interactions and concentrations quenching in the electroluminescence (EL) process could also be sufficiently suppressed. The efficient energy transfer also reduced intermolecular interactions’ contribution to the enhanced device performances compared to the linear single-polymer white-light systems. Moreover, saturated stable white emission results from the joint of energy transfer and trap-assisted recombination. This improved performance is expected to provide the star-like white-emitting copolymers with promising applications for WPLEDs.
Three-Dimensional S/CeO2/RGO Composites as Cathode Materials for Lithium–Sulfur Batteries
Authors: Hao Qiuyan , Cui Guoliang , Tian Yuan , Tan Taizhe , Zhang Yongguang
Published: September 2018

In this paper, the synthesis of the three-dimensional (3D) composite of spherical reduced graphene oxide (RGO) with uniformly distributed CeO2 particles is reported. This synthesis is done via a facile and large-scalable spray-drying process, and the CeO2/RGO materials are hydrothermally compounded with sulfur. The morphology, composition, structure, and electrochemical properties of the 3D S/CeO2/RGO composite are studied using X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), Raman spectra and X-ray photoelectron spectroscopy (XPS), etc. The electrochemical performance of the composites as electrodes for lithium–sulfur batteries is evaluated. The S/CeO2/RGO composites deliver a high initial capacity of 1054 mAh g−1, and retain a reversible capacity of 792 mAh g−1 after 200 cycles at 0.1 C. Profiting from the combined effect of CeO2 and RGO, the CeO2/RGO materials effectively inhibit the dissolution of polysulfides, and the coating of spherical RGO improves the structural stability as well as conductivity.
Freeze–Thaw Durability of Strain-Hardening Cement-Based Composites under Combined Flexural Load and Chloride Environment
Authors: Yin Liqiang , Yan Changwang , Liu Shuguang
Published: September 2018

Cement-based materials are usually not exposed to an independent deterioration process but are exposed to a combination of mechanical load and environmental effects. This paper reports the frost resistance durability of strain-hardening cement-based composites (SHCC) under combined flexural loading at different levels and under chloride attack. The loss of mass, dynamic elastic modulus, and microstructure characteristics of SHCC specimens were determined, and the influence of loading level on frost resistance was analyzed. In addition, the effect of freeze–thaw action on the flexural performance and diffusion properties of chloride in SHCC under the combined loads was investigated. The results show that the process of degradation was accelerated due to the simultaneous action of flexural loading and freeze–thaw cycles in the chloride environment, and SHCC suffered more serious damage at a higher loading level. However, flexural strength decreased by only 13.87% after 300 freeze–thaw cycles at load level S = 0.36. The diffusion properties of chloride in SHCC under constant flexural loading were affected by the freezing and thawing cycle. The free chloride concentration Cf increased with the development of freezing and thawing at the same diffusion depth, and a bilinear relationship was found between the chloride diffusion coefficient Dc and the number of freeze–thaw cycles.
Ion Transport Properties and Ionicity of 1,3-Dimethyl-1,2,3-Triazolium Salts with Fluorinated Anions
Authors: Pulst Martin , Golitsyn Yury , Reichert Detlef , Kressler Jörg
Published: September 2018

1,2,3-Triazolium salts are an important class of materials with a plethora of sophisticated applications. A series of three novel 1,3-dimethyl-1,2,3-triazolium salts with fluorine, containing anions of various size, is synthesized by methylation of 1,2,3-triazole. Their ion conductivity is measured by impedance spectroscopy, and the corresponding ionicities are determined by diffusion coefficients obtained from 1H and 19F pulsed field gradient nuclear magnetic resonance (PFG NMR) spectroscopy data, revealing that the anion strongly influences their ion conductive properties. Since the molar ion conductivities and ionicities of the 1,3-dimethyl-1,2,3-triazolium salts are enhanced in comparison to other 1,2,3-triazolium salts with longer alkyl substituents, they are promising candidates for applications as electrolytes in electrochemical devices.
Thermochromic and Femtosecond-Laser-Induced Damage Performance of Tungsten-Doped Vanadium Dioxide Films Prepared Using an Alloy Target
Authors: Zhu Mao-Dong , Shan Chong , Li Cheng , Wang Hu , Qi Hong-Ji , Zhang Dong-Ping , Lv Wei Zhong
Published: September 2018

Thermochromic tungsten-doped VO2 thin films were successfully fabricated using a W-V alloy target. X-ray diffraction analyses showed that the W-doped VO2 film had a preferred orientation of (011), and that the doping did not degrade the film crystallinity compared with that of the pure film. X-ray photoelectron spectroscopy and energy-dispersive spectroscopy showed that the doped 0.81 atom% tungsten replaced vanadium in the lattice of the film. The metal–insulator transition temperature of the W-doped VO2 film was reduced to 35.5 °C, which is close to room temperature. Additionally, the infrared transmittance modulation of the W-doped film at λ = 2500 nm reached 56%, indicating an excellent switching efficiency. The damage behavior of the W-doped VO2 film under a femtosecond-laser irradiation was experimentally investigated. Our results revealed that defect-related damages induced by the femtosecond laser are relevant for W-doped VO2 films. This study provides valuable insights into VO2 films for potential applications in laser protection.
Thin Electric Heating Membrane Constructed with a Three-Dimensional Nanofibrillated Cellulose–Graphene–Graphene Oxide System
Authors: Shao Chuang , Zhu Zhenyu , Su Chuwang , Yang Sheng , Yuan Quanping
Published: September 2018

Nanofibrillated cellulose (NFC) and graphene oxide (GO) with reinforcing and film-forming properties were employed with graphene to develop a novel and thin electric heating membrane with heat dissipation controllability. A negative charge was found on the surface of GO and NFC in aqueous dispersions, which contributed to the homogeneous distribution of the graphene sheets. The membrane had a good laminated structure with three-dimensional interaction between GO and NFC, with embedded graphene sheets. Conductivity was characterized as a function of the amount of graphene, thus giving control over to the heating power by adjusting the ratio of graphene. Subsequent electric heating tests can remove irregularities on the GO and graphene sheet, improving the laminated structure further. The temperature on the surface of the membrane presented an exponential increasing regularity with time. Under the same power density and time, the stabilized temperature rise of membranes was higher when grammage was higher, which was characterized by the linear function of the power density. Low-grammage membranes (1 and 4 g·m−2) also exhibited regular and even stabilized temperature rises. The indicated structure and heating performance of the membrane, as well as the variation induced by Joule heating, would drive its applications.
Enamel Anti-Demineralization Effect of Orthodontic Adhesive Containing Bioactive Glass and Graphene Oxide: An In-Vitro Study
Authors: Lee Seung-Min , Yoo Kyung-Hyeon , Yoon Seog-Young , Kim In-Ryoung , Park Bong-Soo , Son Woo-Sung , Ko Ching-Chang , Son Sung-Ae , Kim Yong-Il
Published: September 2018

White spot lesions (WSLs), a side effect of orthodontic treatment, can result in reversible and unaesthetic results. Graphene oxide (GO) with a bioactive glass (BAG) mixture (BAG@GO) was added to Low-Viscosity Transbond XT (LV) in a ratio of 1, 3, and 5%. The composite’s characterization and its physical and biological properties were verified with scanning electron microscopy (SEM) and X-ray diffraction (XRD); its microhardness, shear bond strength (SBS), cell viability, and adhesive remnant index (ARI) were also assessed. Efficiency in reducing WSL was evaluated using antibacterial activity of S. mutans. Anti-demineralization was analyzed using a cycle of the acid-base solution. Adhesives with 3 wt.% or 5 wt.% of BAG@GO showed significant increase in microhardness compared with LV. The sample and LV groups showed no significant differences in SBS or ARI. The cell viability test confirmed that none of the sample groups showed higher toxicity compared to the LV group. Antibacterial activity was higher in the 48-h group than in the 24 h group; the 48 h test showed that BAG@GO had a high antibacterial effect, which was more pronounced in 5 wt.% of BAG@GO. Anti-demineralization effect was higher in the BAG@GO-group than in the LV-group; the higher the BAG@GO concentration, the higher the anti-demineralization effect.
Recycled Rubber as an Aggregate Replacement in Self-Compacting Concrete—Literature Overview
Authors: Bušić Robert , Miličević Ivana , Šipoš Tanja Kalman , Strukar Kristina
Published: September 2018

In the past few decades, due to the exponential increase of the world’s population, the number of discarded waste tires has become a serious ecological and environmental problem. Decomposition of waste tire rubber can take longer than 50 years, and every year the number of discarded tires is rapidly growing. With the inclusion of waste tire rubber into self-compacting concrete this global problem can be reduced. Waste tire rubber can be incorporated in self-compacting concrete by partially replacing the natural fine and coarse aggregate, reducing consumption of sand and gravel and preserving these natural materials. In addition, recycling and reusing waste tire rubber avoids the need for tire landfilling, as one of the major ecological problem of the near future. Replacement of natural aggregate with waste tire rubber can have an undesirable influence on the mechanical properties of self-compacting concrete, i.e., compressive strength, flexural strength, splitting tensile strength, and modulus of elasticity, however. On the other hand, replacing natural gravel or sand with waste tire rubber can improve impact resistance, ductility, and fatigue resistance. This paper presents an overview of the literature investigating recycled waste tire rubber used as a fine and/or coarse aggregate replacement in self-compacting concrete and its influence on several essential fresh and hardened self-compacting concrete properties.
Advanced Radar Absorbing Ceramic-Based Materials for Multifunctional Applications in Space Environment
Authors: Delfini Andrea , Albano Marta , Vricella Antonio , Santoni Fabio , Rubini Giulio , Pastore Roberto , Marchetti Mario
Published: September 2018

In this review, some results of the experimental activity carried out by the authors on advanced composite materials for space applications are reported. Composites are widely employed in the aerospace industry thanks to their lightweight and advanced thermo-mechanical and electrical properties. A critical issue to tackle using engineered materials for space activities is providing two or more specific functionalities by means of single items/components. In this scenario, carbon-based composites are believed to be ideal candidates for the forthcoming development of aerospace research and space missions, since a widespread variety of multi-functional structures are allowed by employing these materials. The research results described here suggest that hybrid ceramic/polymeric structures could be employed as spacecraft-specific subsystems in order to ensure extreme temperature withstanding and electromagnetic shielding behavior simultaneously. The morphological and thermo-mechanical analysis of carbon/carbon (C/C) three-dimensional (3D) shell prototypes is reported; then, the microwave characterization of multilayered carbon-filled micro-/nano-composite panels is described. Finally, the possibility of combining the C/C bulk with a carbon-reinforced skin in a synergic arrangement is discussed, with the aid of numerical and experimental analyses.
Preparation and Characteristics of Biocomposites Based on Steam Exploded Sisal Fiber Modified with Amphipathic Epoxidized Soybean Oil Resin
Authors: Lei Bo , Liang Yong , Feng Yanhong , He Hezhi , Yang Zhitao
Published: September 2018

Sisal fiber was pretreated by continuous screw extrusion steam explosion to prepare steam exploded sisal fiber (SESF) preforms. An amphipathic bio-based thermosetting resin with poor mechanical properties was cured by epoxidized soybean oil (ESO) and citric acid (CA). The obtained resin was used to modify SESF preforms and prepare eco-friendly biocomposites. The molar ratios (R) of carboxylic groups to epoxy groups and resin contents in biocomposites were adjusted. The biocomposites were characterized by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transfer infrared spectroscopy (FT-IR), tensile testing, scanning electron microscopy (SEM), water absorption and water contact angle measurements. The maximum thermal decomposition temperature of the biocomposites was 373.1 °C. The curing efficiency of the resin in the biocomposites improved with the increase of resin content, and reached a maximum at R = 1.2. The tensile strength of the biocomposites reached a maximum of 30.4 MPa at R = 1.2 and 40% resin content. SEM images showed excellent interfacial bonding and fracture mechanisms within the biocomposites. The biocomposites exhibited satisfactory water resistance. ESO resin cured with polybasic carboxylic acid is therefore a good bio-based modifier for lignocellulose, that prepare biocomposites with good mechanical properties, hydrophobicity, and thermostability, and which has a potential application in packaging.
Biomimetic Mineralizing Agents Recover the Micro Tensile Bond Strength of Demineralized Dentin
Authors: Barbosa-Martins Luiz Filipe , Sousa Jossaria Pereira de , Alves Lívia Araújo , Davies Robert Philip Wynn , Puppin-Rontanti Regina Maria
Published: September 2018

Biomimetic remineralization is an approach that mimics natural biomineralization, and improves adhesive procedures. The aim of this paper was to investigate the influence of Dentin Caries-like Lesions (DCLL)-Producing Model on microtensile bond strength (μTBS) of etch and rinse adhesive systems and investigate the effect of remineralizing agents such as Sodium Fluoride (NaF), MI Paste™ (MP) and Curodont™ Repair (CR) on caries-affected dentin (n = 6). Nine groups were established: (1) Sound dentin; (2) Demineralized dentin/Chemical DCLL: (3) Demineralized dentin/Biological DCLL; (4) Chemical/DCLL + NaF; (5) Chemical/DCLL + MP; (6) Chemical/DCLL + CR; (7) Biological/DCLL + NaF; (8) Biological/DCLL + MP; (9) Biological/DCLL + CR. Then all dentin blocks were subjected to a bonding procedure with Adper™ Single Bond 2 adhesive system/Filtek Z350XT 4 mm high block, following this they were immersed in deionized water/24 h and then sectioned with ≅1 mm2 beams. The μTBS test was conducted at 1 mm/min/500 N loading. Failure sites were evaluated by SEM (scanning electron microscopy (150×). μTBS data were submitted to factorial ANOVA and Tukey’s test (p < 0.05). The highest values were found when demineralized dentin was treated with MP and CR, regardless caries lesion depth (p < 0.05). There was a predominance of adhesive/mixed in the present study. It was concluded that the use of the artificial dentin caries production models produces differences in the μTBS. Additionally MP and CR remineralizing agents could enhance adhesive procedures even at different models of caries lesion.