bluuebunny/crossref_metadata_2025 huggingface dataset:
Seismic Effectiveness of Multiple Seismic Measures on a Continuous Girder Bridge
Authors: Zhang Yumin , Shi Yun , Liu Dengke
Published: January 2020

Seismic hazards, such as bridge pounding, unseating, collapse, etc., cause significant economic losses and affect traffic and safety. Research on seismic measures, such as limiting and unseating prevention devices for the bridge, can effectively prevent damage to the bearings, such as excessive displacement, the pounding of the beam end, etc., in an earthquake. In this paper, the dynamic time-history analysis method was used to study the mechanical behaviors of the bridge structure, such as its seismic performance, structural displacement, pier bending moment, etc. We found that different combinations of seismic measures can effectively reduce the displacement at the bridge expansion joint and bearings. The joint application of an expansion device, restrainer, and unseating prevention devices shows the best limiting effect on bridge displacement and expansion joint displacement. The maximum reduction of bridge expansion joint displacement reaches 48% and is within the allowable deformation range of an expansion device in a large earthquake, and the maximum reduction of bearing displacement reaches 34%, which only slightly exceeds the shear deformation of the bearing. The expansion device, restrainer, and unseating prevention devices have smaller internal forces in this case than other cases, without damage. In contrast to the previous studies on single seismic measures of unseating restrainers, this study investigates the combination of multiple seismic measures and earthquakes of various magnitude. It reveals the catastrophe process of the bridge structure and the cooperation law of seismic measures in an earthquake.
Comparison of Different Biofilter Media During Biological Bed Maturation Using Common Carp as a Biogen Donor
Authors: Sikora Mateusz , Nowosad Joanna , Kucharczyk Dariusz
Published: January 2020

This experiment analysed the operation of submerged and dripping biological filters with three types of filling: commercial fitting HXF12KLL (CF), two innovative polypropylene aggregates (PPA) and polyethylene screw caps for PET bottles (PSC). The experiment determined the time needed to reach full filter functionality at the maturation stage, the time needed to start successive stages of the nitrification process and the maximum concentration of each nitrogen compound in water in the recirculation systems. The filter operation characteristics after the maturation stage were also examined. These issues are crucial during the preparation and launch of new aquaculture facilities. A literature analysis indicated that the ability of biological filters to oxidise nitrogen compounds is affected by a number of factors. Studies conducted at various centres have covered selected aspects and factors affecting the effectiveness of biological filters. During this study, the model fish common carp (Cyprinus carpio) was used. The current experiment involved examination of biological filter maturation and operation during the carp fry rearing stage, which allowed the biofilter operation characteristics to be determined. At the third day of the experiment, the ammonium concentration reached approximately 3 mg NH4-N/dm3. It remained at this level for 10 days and later decreased below 0.25 mg NH4-N/dm3. The maximum nitrite concentration ranged from 11.7 mg/dm3 to 20.9 mg NO2-N/dm3 within 9 to 20 days and later decreased with time. Nitrate concentrations were seen to increase during the experiment. The all applied biofilter media showed possibility to be used in commercial aquaculture systems.
Analysis of the Cause and Mechanism of Hydraulic Gate Vibration during Flood Discharging from the Perspective of Structural Dynamics
Authors: Lian Jijian , Chen Lin , Ma Bin , Liang Chao
Published: January 2020

According to the results of a dynamic prototype test for the surface outlet radial gate on the Jinping high arch dam during the flood discharging process, a novel cause of vibration fundamentally different from the traditional causes of flow-induced radial gate vibration, is analyzed for the first time. Under the condition that the flood is discharged only from mid-level outlets, an accompanying vibration of the surface outlet gate is induced by the vibration of the closely spaced mid-level outlet gates. It is counterintuitive that the most intense vibration occurs when the surface outlet gate is closed and, on the contrary, the vibration is reduced when the gate is opened and subjected to flow excitation. In order to analyze and explain this accompanying vibration phenomenon, a theoretical model is developed based on the conventional theory of passive vibration absorbers. The difference between the proposed and conventional theoretical models is that more complex load and damping conditions are considered, and more attention was paid to the dynamic behavior of the accessory structure. Then, the cause and mechanism for the surface outlet gate vibration is clarified in detail, based on the proposed theoretical model. The comprehensive analysis and mutual verification of the prototype test, theoretical derivation and numerical simulation, indicate that the clarification and the proposed theoretical model is reasonable and accurate. The research reported in this paper will be beneficial for the design, operation and maintenance of the hydraulic gates installed on high arch dams.
Antibiotic Potential and Chemical Composition of the Essential Oil of Piper caldense C. DC. (Piperaceae)
Authors: Bezerra José Weverton Almeida , Rodrigues Felicidade Caroline , Pereira da Cruz Rafael , Silva Luiz Everson da , do Amaral Wanderlei , Andrade Rebelo Ricardo , Begnini Ieda Maria , Fonseca Bezerra Camila , Iriti Marcello , Varoni Elena Maria , Melo Coutinho Henrique Douglas , Bezerra Morais-Braga Maria Flaviana
Published: January 2020

Infections by multiresistant microorganisms have led to a continuous investigation of substances acting as modifiers of this resistance. By following this approach, the chemical composition of the essential oil from Piper caldense leaf and its antimicrobial potential were investigated. The antimicrobial activity was determined by broth microdilution method providing values for minimum inhibitory concentration (MIC), IC50, and minimum fungicidal concentration (MFC). The essential oil was tested as a modulator for several antibiotics, and its effect on the morphology of Candida albicans (CA) strains was also investigated. The chemical characterization revealed an oil composed mainly of sesquiterpenes. Among them are caryophyllene oxide (13.9%), spathulenol (9.1%), δ-cadinene (7.6%) and bicyclogermacrene (6.7%) with the highest concentrations. The essential oil showed very low activity against the strains of CA with the lowest values for IC50 and MFC of 1790 μg/mL and 8192 μg/mL, respectively. The essential oil modulated the activity of fluconazole against CA URM 4387 strain, which was demonstrated by the lower IC50 obtained, 2.7 μg/mL, whereas fluconazole itself presented an IC50 of 7.76 μg/mL. No modulating effect was observed in the MFC bioassays. The effect on fungal morphology was observed for both CA INCQS 40006 and URM 4387 strains. The hyphae projection was completely inhibited at 4096 μg/mL and 2048 μg/mL, respectively. Thus, the oil has potential as an adjuvant in antimicrobial formulations.
Effects of Parameters of Air-Avid Structure on the Salt-Frost Durability of Hardened Concrete
Authors: Zhang Hui , Gao Peiwei , Zhang Zhixiang , Pan Youqiang , Zhang Weiguang
Published: January 2020

Through laboratory testing, this research studied the connection between air-void structures of hardened concrete and fresh concrete and discussed the effects of the air-void structure on the salt-frost durability of the concrete. The results demonstrate that, in comparison with fresh concrete, the air-void spacing factor shows a close correlation with hardened concrete air-content and decreases in the form of a power function as the air-content increases. When the fresh concrete air-content is more than 6% and the hardened concrete air-void spacing factor is less than 0.18 mm, the influence of parameters of air-void structure on the salt-frost resistance of the concrete reduces. The air-void spacing factor more significantly affects the salt-frost resistance of the concrete compared with air content and the correlation reaches 0.93. Therefore, air-content and air-void spacing factor are recommended for dual control.
A Review on Mixed Reality: Current Trends, Challenges and Prospects
Authors: Rokhsaritalemi Somaiieh , Sadeghi-Niaraki Abolghasem , Choi Soo-Mi
Published: January 2020

Currently, new technologies have enabled the design of smart applications that are used as decision-making tools in the problems of daily life. The key issue in designing such an application is the increasing level of user interaction. Mixed reality (MR) is an emerging technology that deals with maximum user interaction in the real world compared to other similar technologies. Developing an MR application is complicated, and depends on the different components that have been addressed in previous literature. In addition to the extraction of such components, a comprehensive study that presents a generic framework comprising all components required to develop MR applications needs to be performed. This review studies intensive research to obtain a comprehensive framework for MR applications. The suggested framework comprises five layers: the first layer considers system components; the second and third layers focus on architectural issues for component integration; the fourth layer is the application layer that executes the architecture; and the fifth layer is the user interface layer that enables user interaction. The merits of this study are as follows: this review can act as a proper resource for MR basic concepts, and it introduces MR development steps and analytical models, a simulation toolkit, system types, and architecture types, in addition to practical issues for stakeholders such as considering MR different domains.
Experimental Study on the Torsional Behaviour of Prestressed HSC Hollow Beams
Authors: Bernardo Luís , Lopes Sérgio , Teixeira Mafalda
Published: January 2020

This article describes an experimental program developed to study the influence of longitudinal prestress on the behaviour of high-strength concrete hollow beams under pure torsion. The pre-cracking, the post-cracking and the ultimate behaviour are analysed. Three tests were carried out on large hollow high-strength concrete beams with similar concrete strength. The variable studied was the level of longitudinal uniform prestress. Some important conclusions on different aspects of the beams’ behaviour are presented. These conclusions, considered important for the design of box bridges, include the influence of the level of prestress in the cracking and ultimate behaviour.
Carbon Dioxide Uptake by Mortars and Concretes Made with Portuguese Cements
Authors: Sanjuán Miguel Ángel , Andrade Carmen , Mora Pedro , Zaragoza Aniceto
Published: January 2020

As the cement industry continues to address its role in the climate crisis, Portugal’s cement industry has started to calculate its net CO2 emissions to become an entirely carbon neutral sector. These emissions are calculated by simply subtracting the total CO2 uptake due to mortar and concrete carbonation from the total CO2 that is emitted during the calcination process (clinker production). However, the procedures given in the Intergovernmental Panel on Climate Change (IPCC) Guidelines for National Greenhouse Gas (GHG) Inventories to report GHG emissions do not contain any element that would grant this calculation method the status of an internationally recognized procedure. Therefore, some climate models are not accurate because they do not account for the carbon dioxide uptake due to concrete and mortar carbonation, as is evidenced in this paper. Climate models have improved since the IPCC’s Fourth Assessment Report (AR4), but they can further improve by implementing carbon dioxide uptake by cement-based materials. In the present paper, a quick and easy method of evaluating net CO2 emissions is utilized (simplified method) along with an advanced method. Portuguese net CO2 emissions of the cement produced from 2005 to 2015 were calculated while taking carbon dioxide uptake during the service-life and end-of-life and secondary usage stages into account. Following the simplified method, 8.7 million tons of carbon dioxide were found to be uptake by mortars and concretes made with Portuguese cement over the ten-year period, in which 37.8 million tons were released due to the calcination process. In addition, an advanced method has been used to estimate the carbon dioxide uptake, which provided only slightly higher results than that of the simplified method (9.1 million tons).
Heat Transfer Performance of Fruit Juice in a Heat Exchanger Tube Using Numerical Simulations
Authors: Córcoles Juan Ignacio , Marín-Alarcón Ernesto , Almendros-Ibáñez Jose Antonio
Published: January 2020

Enhancing heat transfer rates in heat exchangers is essential in many applications, such as in the food industry. Most fluids used in the food industry are non-Newtonian, whose viscosity is not uniform, and depends on the shear rate and temperature gradient. This is important in the selection of equipment and type of processing. The aim of this work was to numerically simulate, with a non-Newtonian fluid in laminar regime, the heat transfer process in a tube with a curved elbow. The numerical model was validated with published correlations using water as heat transfer fluid. A commercially available fruit juice was used as a non-Newtonian fluid. Its rheological properties were measured using a Modular Compact Rheometer, as well as the activation energy. The difference between outlet temperature and inlet temperature was higher for the laminar simulation (approximately 4 °C) than for the turbulent one (approximately 0.7 °C). The highest dynamic viscosity values were found at the centre of the pipe (between 0.05 and 0.09 Pa·s), with the lowest values at the wall (0.0076 Pa·s). This behaviour is explained by the pseudoplastic condition of the fruit juice. The activation energy did not yield high values, showing a moderate viscosity variation with the temperature change.
Amplification of Radiation-Induced Signal of LED Strip by Increasing Number of LED Chips and Using Amplifier Board
Authors: Damulira Edrine , Yusoff Muhammad Nur Salihin , Omar Ahmad Fairuz , Taib Nur Hartini Mohd
Published: January 2020

Transducers, such as photodiodes, phototransistors, and photovoltaic cells are promising radiation detectors. However, for accurate radiation detection and dosimetry, signals that emanate from these devices have to be sufficient to facilitate accurate calibrations, i.e., assigning a quantity of radiation dose to a specific magnitude of the signal. More so, purposely fabricated for luminescence, LEDs produce significantly low signals during radiation detection applications. Therefore, this paper investigates the enhancement and augmentation of photovoltaic signals that were generated when LED strips were being exposed to diagnostic X-rays. Initially, signal amplification was achieved through increasing the effective LED active area (from 60 to 120 chips); by successively connecting LED strips. Further, signal amplification was undertaken by injecting the raw LED strip signal into an amplifier board with adjustable gains. In both the signal amplification techniques, the tube voltage (kVp), tube current-time product (mAs), and source-to-detector distance (SDD) were varied. The principal findings show that effective active area-based signal amplifications produced an overall average of 91.16% signal enhancement throughout all of the X-ray parameter variations. On the other hand, the amplifier board produced an average of 36.48% signal enhancement for the signals that were injected into it. Chip number increment-based signal amplifications had a 0.687% less coefficient of variation than amplifier board signal amplifications. The amplifier board signal amplifications were impaired by factors, such as dark currents, amplifier board maximum operational output voltage, and saturation. Therefore, future electronic signal amplification could use amplifier boards having low dark currents and high operational voltage headroom. The low-cost and simplicity that are associated with active-area amplification could be further exploited in a hybrid amplification technique with electronic amplification and scintillators.
Accurate and Rapid Auto-Focus Methods Based on Image Quality Assessment for Telescope Observation
Authors: Yang Chunping , Chen Minhao , Zhou Fangfang , Li Wei , Peng Zhenming
Published: January 2020

Aiming at improving the speed and accuracy of auto-focus for telescope observation, algorithms for image estimation and auto-focus were investigated and are discussed in this article. Based on the image quality assessment, the auto-focusing process of the telescope system is realized by using the mountain-climb search method. Several evaluation functions were tested in different scenarios. It is demonstrated that the Tenengrad image estimation function (IEF) is suitable for an instant and accurate auto-focus process of the telescope. Furthermore, we implemented sampling and dynamic adaptive focusing window (ES-DAFW) methods with the Tenengrad IEF to enhance the sensitivity and accuracy of the auto-focus process. The experimental results showed that our ES-DATW method can provide more accurate results in less time for the auto-focus process compared to the conventional approaches, especially for a sparse image. These results promise significant applications to the auto-focusing of other telescopes with image quality assessment.
Influence of Laser Intensity Fluctuation on Single-Cesium Atom Trapping Lifetime in a 1064-nm Microscopic Optical Tweezer
Authors: Sun Rui , Wang Xin , Zhang Kong , He Jun , Wang Junmin
Published: January 2020

An optical tweezer composed of a strongly focused single-spatial-mode Gaussian beam of a red-detuned 1064-nm laser can confine a single-cesium (Cs) atom at the strongest point of the light intensity. We can use this for coherent manipulation of single-quantum bits and single-photon sources. The trapping lifetime of the atoms in the optical tweezers is very short due to the impact of the background atoms, the parametric heating of the optical tweezer and the residual thermal motion of the atoms. In this paper, we analyzed the influence of the background pressure, the trap frequency of optical tweezers and the laser intensity fluctuation of optical tweezers on the atomic trapping lifetime. Combined with the external feedback loop based on an acousto-optical modulator (AOM), the intensity fluctuation of the 1064-nm laser in the time domain was suppressed from ±3.360% to ±0.064%, and the suppression bandwidth in the frequency domain reached approximately 33 kHz. The trapping lifetime of a single-Cs atom in the microscopic optical tweezers was extended from 4.04 s to 6.34 s.
Using Statistical Analysis of an Acceleration-Based Bridge Weigh-In-Motion System for Damage Detection
Authors: OBrien Eugene , Khan Muhammad Arslan , McCrum Daniel Patrick , Žnidarič Aleš
Published: January 2020

This paper develops a novel method of bridge damage detection using statistical analysis of data from an acceleration-based bridge weigh-in-motion (BWIM) system. Bridge dynamic analysis using a vehicle-bridge interaction model is carried out to obtain bridge accelerations, and the BWIM concept is applied to infer the vehicle axle weights. A large volume of traffic data tends to remain consistent (e.g., most frequent gross vehicle weight (GVW) of 3-axle trucks); therefore, the statistical properties of inferred vehicle weights are used to develop a bridge damage detection technique. Global change of bridge stiffness due to a change in the elastic modulus of concrete is used as a proxy of bridge damage. This approach has the advantage of overcoming the variability in acceleration signals due to the wide variety of source excitations/vehicles—data from a large number of different vehicles can be easily combined in the form of inferred vehicle weight. One year of experimental data from a short-span reinforced concrete bridge in Slovenia is used to assess the effectiveness of the new approach. Although the acceleration-based BWIM system is inaccurate for finding vehicle axle-weights, it is found to be effective in detecting damage using statistical analysis. It is shown through simulation as well as by experimental analysis that a significant change in the statistical properties of the inferred BWIM data results from changes in the bridge condition.
Micro-Slit Cutting in an Aluminum Foil Using an Un-Traveling Tungsten Wire
Authors: Zhu Jiacheng , Fang Xiaolong , Qu Ningsong
Published: January 2020

Microslit cutting in aluminum foils is considered to be difficult as aluminum foils have low hardness and deformability. In this study, a novel cutting method is proposed where a tungsten microwire is utilized as the tool to cut aluminum foil without tool traveling or spinning. A statics simulation is first performed to analyze the cutting mechanism. Further, a tungsten wire with a diameter of 50 μm is utilized as the tool and a series of experiments are carried to discuss how the feeding rate influences slit width and roughness. With optimal parameters, it takes only 100 s to fabricate a 5 mm long microslit with an average width of 48.75 μm, width standard deviation of 1.48 μm, and surface roughness of 0.110 μm when applying initial/secondary velocity of 50/50 μm·s−1.
Conceptual Framework of an Intelligent Decision Support System for Smart City Disaster Management
Authors: Jung Daekyo , Tran Tuan Vu , Quoc Tran Dai , Park Minsoo , Park Seunghee
Published: January 2020

In order to protect human lives and infrastructure, as well as to minimize the risk of damage, it is important to predict and respond to natural disasters in advance. However, currently, the standardized disaster response system in South Korea still needs further advancement, and the response phase systems need to be improved to ensure that they are properly equipped to cope with natural disasters. Existing studies on intelligent disaster management systems (IDSSs) in South Korea have focused only on storms, floods, and earthquakes, and they have not used past data. This research proposes a new conceptual framework of an IDSS for disaster management, with particular attention paid to wildfires and cold/heat waves. The IDSS uses big data collected from open application programming interface (API) and artificial intelligence (AI) algorithms to help decision-makers make faster and more accurate decisions. In addition, a simple example of the use of a convolutional neural network (CNN) to detect fire in surveillance video has been developed, which can be used for automatic fire detection and provide an appropriate response. The system will also consider connecting to open source intelligence (OSINT) to identify vulnerabilities, mitigate risks, and develop more robust security policies than those currently in place to prevent cyber-attacks.
Modelling of Electromagnetic Energy Harvester with Rotational Pendulum Using Mechanical Vibrations to Scavenge Electrical Energy
Authors: Ambrożkiewicz Bartłomiej , Litak Grzegorz , Wolszczak Piotr
Published: January 2020

A concept of non-linear electromagnetic system with the rotational magnetic pendulum for energy harvesting from mechanical vibrations was presented. The system was stimulated by vertical excitation coming from a shaker. The main assumption of the system was the montage of additional regulated stationary magnets inside coils creating double potential well, and the system was made with a 3D printing technique in order to avoid a magnetic coupling with the housing. In validation process of the system, modelling of electromagnetic effects in different configurations of magnets positions was performed with the application of a finite element method (FEM) obtaining the value of magnetic force acting on the pendulum. A laboratory measurement circuit was built and an experiment was carried out. The voltage and power outputs were measured for different excitations in range of system operational frequencies found experimentally. The experimental results of the physical system with electrical circuit and numerical estimations of the magnetic field of a stationary magnet’s configuration were used to derive a mathematical model. The equation of motion for the rotational pendulum was used to prove the broadband frequency effect.
Resilience Analysis for Double Spending via Sequential Decision Optimization
Authors: Hinz Juri
Published: January 2020

Recently, diverse concepts originating from blockchain ideas have gained increasing popularity. One of the innovations in this technology is the use of the proof-of-work (PoW) concept for reaching a consensus within a distributed network of autonomous computer nodes. This goal has been achieved by design of PoW-based protocols with a built-in equilibrium property: If all participants operate honestly then the best strategy of any agent is also to follow the same protocol. However, there are concerns about the stability of such systems. In this context, the analysis of attack vectors, which represent potentially successful deviations from the honest behavior, turns out to be the most crucial question. Naturally, stability of a blockchain system can be assessed only by determining its most vulnerable components. For this reason, knowing the most successful attacks, regardless of their sophistication level, is inevitable for a reliable stability analysis. In this work, we focus entirely on blockchain systems which are based on the proof-of-work consensus protocols, referred to as PoW-based systems, and consider planning and launching an attack on such system as an optimal sequential decision-making problem under uncertainty. With our results, we suggest a quantitative approach to decide whether a given PoW-based system is vulnerable with respect to this type of attack, which can help assessing and improving its stability.
Low Polluting Building Materials and Ventilation for Good Air Quality in Residential Buildings: A Cost–Benefit Study
Authors: Babich Francesco , Demanega Ingrid , Avella Francesca , Belleri Annamaria
Published: January 2020

Nowadays, people spend an average of 87% of their time inside buildings, and about 69% at home. Hence, it is essential to ensure the highest possible level of indoor air quality (IAQ). Providing that the quality of the outdoor air is acceptable, the IAQ level is improved by increasing the ventilation rates. However, this means that a larger volume of air must be cooled down or warmed up to ensure the same level of thermal comfort. The aim of this study was to conduct a cost–benefit analysis of the IAQ in residential buildings. A case-study building was defined, and three sets of materials with different pollution emission levels were chosen: High, low, and very low. For each option, the ventilation rates required to have the same IAQ level were calculated, and the consequent energy consumption and costs were estimated by means of dynamic thermal simulation. The results show the range of the initial capital cost that could be compensated for by lower running costs, and the effect of each energy and economic input assumption on the appraisal of the affordable capital cost. In the discussion, insights into the IAQ co-benefits are also given.
Association of Diurnal Rainfall in Northeastern Tibetan Plateau with the Retreat of the South Asian High
Authors: Zhao Lin , Wang S.-Y. Simon , Wu Chi-Hua , Los Sebastian , Lyu Shihua , Meng Xianhong , Wen Lijuan , Luo Siqiong , Ao Yinhuan , Li Zhaoguo
Published: January 2020

The characteristics of intense diurnal precipitation occurring beneath the South Asian High (SAH) are diagnosed in the summer monsoon season from 2010 to 2015 using observational data. The diagnostics indicate that summer nighttime rainfall events in the northeastern Tibetan Plateau can intensify towards the end of the monsoon period. By defining a transition index to identify the transition day during which the episodes of diurnal convection start to decline, daily thermodynamic properties and precipitation from each year were composited before and after the transition date. The analysis reveals that warmer air, increased moisture, and stronger upward velocity are present in the atmosphere before the transition day, potentially elevating nighttime convective precipitation. Enhanced upward velocity that is present through the two months prior to transition date coincides with the timing of the peak SAH, while weakened upward velocity afterwards coincides with its subsequent retreat. The large-scale lift due to terrain-ambient air interaction underneath the SAH and the increased moisture content can enhance the potential for diurnal convection, which lends support to the nighttime peak of rainfall. This feature persists until the transition date, after which the SAH starts to retreat.
Assessment of a Real-Time Prediction Method for High Clothing Thermal Insulation Using a Thermoregulation Model and an Infrared Camera
Authors: Lee Kyungsoo , Choi Haneul , Kim Hyungkeun , Kim Daeung Danny , Kim Taeyeon
Published: January 2020

For evaluating the thermal comfort of occupants, human factors such as clothing thermal insulation (clo level) and metabolic rate (Met) are one of the important parameters as well as environmental factors such as air temperature (Ta) and humidity. In general, a fixed clo level is commonly used for controlling heating, ventilation, and air conditioning using the thermal comfort index. However, a fixed clo level can lead to errors for estimating the thermal comfort of occupants, because clo levels of occupants can vary with time and by season. The present study assesses a method for predicting the clo level of occupants using a thermoregulation model and an infrared (IR) camera. The Tanabe model and the Fanger model were used as the thermoregulation models, and the predicted performance for high clo level (winter clothing) was compared. The skin and clothing temperatures of eight subjects using a non-contact IR camera were measured in a climate chamber. In addition, the measured values were used for the thermoregulation models to predict the clo levels. As a result, the Tanabe model showed a better performance than the Fanger model for predicting clo levels. In addition, all models tended to predict a clo level higher than the traditional method.
The Climatology and Trend of Surface Wind Speed over Antarctica and the Southern Ocean and the Implication to Wind Energy Application
Authors: Yu Lejiang , Zhong Shiyuan , Sun Bo
Published: January 2020

Surface wind trends and variability over Antarctica and the Southern Ocean and their implications to wind energy in the region are analyzed using the gridded ERA-Interim reanalysis data between 1979 and 2017 and the Self-Organizing Map (SOM) technique. In general, surface winds are stronger over the coastal regions of East Antarctica and the Transantarctic Mountains and weaker over the Ross and Ronne ice shelves and the Antarctic Peninsula; and stronger in winter and weaker in summer. Winds in the southern Indian and Pacific Oceans and along coastal regions exhibit a strong interannual variability that appears to be correlated to the Antarctic Oscillation (AAO) index. A significantly positive trend in surface wind speeds is found across most regions and about 20% and 17% of the austral autumn and summer wind trends, respectively, and less than 1% of the winter and spring wind trends may be explained by the trends in the AAO index. Except for the Antarctic Peninsula, Ronne and Ross ice shelves, and small areas in the interior East Antarctica, most of the continent is found to be suitable for the development of wind power.
Axiomatic Approach in the Analytic Theory of Singular Perturbations
Authors: Besova Margarita , Kachalov Vasiliy
Published: January 2020

Introduced by S.A. Lomov, the concept of a pseudoanalytic (pseudoholomorphic) solution laid the foundation for the development of the singular perturbation analytical theory. In order for this concept to work in case of linear problems, an apparatus for the theory of exponential type vector spaces was developed. When considering nonlinear singularly perturbed problems, an algebraic approach is currently used. This approval is based on the properties of algebra homomorphisms for holomorphic functions with various numbers of variables, as a result of which it is possible to obtain pseudoholomorphic solutions. In this paper, formally singularly perturbed equations are considered in topological algebras, which allows the authors to formulate the main concepts of the singular perturbation analytical theory from the standpoint of maximal generality.
Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of Thalassiosira Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series
Authors: Rynearson Tatiana A. , Flickinger Sarah A. , Fontaine Diana N.
Published: January 2020

Diatoms generate nearly half of marine primary production and are comprised of a diverse array of species that are often morphologically cryptic or difficult to identify using light microscopy. Here, species composition and realized thermal niches of species in the diatom genus Thalassiosira were examined at the site of the Narragansett Bay (NBay) Long-Term Plankton Time Series using a combination of light microscopy (LM), high-throughput sequencing (HTS) of the 18S rDNA V4 region and historical records. Thalassiosira species were identified over 6 years using a combination of LM and DNA sequences. Sixteen Thalassiosira taxa were identified using HTS: nine were newly identified in NBay. Several newly identified species have small cell diameters and are difficult to identify using LM. However, they appeared frequently and thus may play a significant ecological role in NBay, particularly since their realized niches suggest they are eurythermal and able to tolerate the >25 °C temperature range of NBay. Four distinct species assemblages that grouped by season were best explained by surface water temperature. When compared to historical records, we found that the cold-water species Thalassiosira nordenskioeldii has decreased in persistence over time, suggesting that increasing surface water temperature has influenced the ecology of phytoplankton in NBay.
Simultaneous Suppression of Two Distinct Serotonin N-Acetyltransferase Isogenes by RNA Interference Leads to Severe Decreases in Melatonin and Accelerated Seed Deterioration in Rice
Authors: Hwang Ok Jin , Back Kyoungwhan
Published: January 2020

Serotonin N-acetyltransferase (SNAT) is the penultimate enzyme in the melatonin biosynthetic pathway, in which serotonin is converted into N-acetylserotonin (NAS) in plants. To date, two SNAT isogenes with low amino acid sequence homologies have been identified. Their single suppression in rice has been reported, but their double suppression in rice has not yet been attempted. Here, we generated double-suppression transgenic rice (snat1+2) using the RNA interference technique. The snat1+2 exhibited retarded seedling growths in conjunction with severe decreases in melatonin compared to wild-types and single-suppression rice plants (snat1 or snat2). The laminar angle was decreased in the snat1+2 rice compared to that of the wild-types and snat1, but was comparable to that of snat2. The reduced germination speed in the snat1+2 was comparable to that of snat2. Seed-aging testing revealed that snat1 was the most severely deteriorated, followed by snat1+2 and snat2, suggesting that melatonin is positively involved in seed longevity.
In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation
Authors: Kulyyassov Arman , Ogryzko Vasily
Published: January 2020

Protein–protein interactions of core pluripotency transcription factors play an important role during cell reprogramming. Cell identity is controlled by a trio of transcription factors: Sox2, Oct4, and Nanog. Thus, methods that help to quantify protein–protein interactions may be useful for understanding the mechanisms of pluripotency at the molecular level. Here, a detailed protocol for the detection and quantitative analysis of in vivo protein–protein proximity of Sox2 and Oct4 using the proximity-utilizing biotinylation (PUB) method is described. The method is based on the coexpression of two proteins of interest fused to a biotin acceptor peptide (BAP)in one case and a biotin ligase enzyme (BirA) in the other. The proximity between the two proteins leads to more efficient biotinylation of the BAP, which can be either detected by Western blotting or quantified using proteomics approaches, such as a multiple reaction monitoring (MRM) analysis. Coexpression of the fusion proteins BAP-X and BirA-Y revealed strong biotinylation of the target proteins when X and Y were, alternatively, the pluripotency transcription factors Sox2 and Oct4, compared with the negative control where X or Y was green fluorescent protein (GFP), which strongly suggests that Sox2 and Oct4 come in close proximity to each other and interact.
Synthetically Lethal Interactions of Heme Oxygenase-1 and Fumarate Hydratase Genes
Authors: Podkalicka Paulina , Mucha Olga , Kruczek Szczepan , Biela Anna , Andrysiak Kalina , Stępniewski Jacek , Mikulski Maciej , Gałęzowski Michał , Sitarz Kamil , Brzózka Krzysztof , Józkowicz Alicja , Dulak Józef , Łoboda Agnieszka
Published: January 2020

Elevated expression of heme oxygenase-1 (HO-1, encoded by HMOX1) is observed in various types of tumors. Hence, it is suggested that HO-1 may serve as a potential target in anticancer therapies. A novel approach to inhibit HO-1 is related to the synthetic lethality of this enzyme and fumarate hydratase (FH). In the current study, we aimed to validate the effect of genetic and pharmacological inhibition of HO-1 in cells isolated from patients suffering from hereditary leiomyomatosis and renal cell carcinoma (HLRCC)—an inherited cancer syndrome, caused by FH deficiency. Initially, we confirmed that UOK 262, UOK 268, and NCCFH1 cell lines are characterized by non-active FH enzyme, high expression of Nrf2 transcription factor-regulated genes, including HMOX1 and attenuated oxidative phosphorylation. Later, we demonstrated that shRNA-mediated genetic inhibition of HMOX1 resulted in diminished viability and proliferation of cancer cells. Chemical inhibition of HO activity using commercially available inhibitors, zinc and tin metalloporphyrins as well as recently described new imidazole-based compounds, especially SLV-11199, led to decreased cancer cell viability and clonogenic potential. In conclusion, the current study points out the possible relevance of HO-1 inhibition as a potential anti-cancer treatment in HLRCC. However, further studies revealing the molecular mechanisms are still needed.
Mechanistic Pathways and Molecular Targets of Plant-Derived Anticancer ent-Kaurane Diterpenes
Authors: Sarwar Md. Shahid , Xia Yi-Xuan , Liang Zheng-Ming , Tsang Siu Wai , Zhang Hong-Jie
Published: January 2020

Since the first discovery in 1961, more than 1300 ent-kaurane diterpenoids have been isolated and identified from different plant sources, mainly the genus Isodon. Chemically, they consist of a perhydrophenanthrene subunit and a cyclopentane ring. A large number of reports describe the anticancer potential and mechanism of action of ent-kaurane compounds in a series of cancer cell lines. Oridonin is one of the prime anticancer ent-kaurane diterpenoids that is currently in a phase-I clinical trial in China. In this review, we have extensively summarized the anticancer activities of ent-kaurane diterpenoids according to their plant sources, mechanistic pathways, and biological targets. Literature analysis found that anticancer effect of ent-kauranes are mainly mediated through regulation of apoptosis, cell cycle arrest, autophagy, and metastasis. Induction of apoptosis is associated with modulation of BCL-2, BAX, PARP, cytochrome c, and cleaved caspase-3, -8, and -9, while cell cycle arrest is controlled by cyclin D1, c-Myc, p21, p53, and CDK-2 and -4. The most common metastatic target proteins of ent-kauranes are MMP-2, MMP-9, VEGF, and VEGFR whereas LC-II and mTOR are key regulators to induce autophagy.
Biofunctionalization of Natural Fiber-Reinforced Biocomposites for Biomedical Applications
Authors: Tavares Tânia D. , Antunes Joana C. , Ferreira Fernando , Felgueiras Helena P.
Published: January 2020

In the last ten years, environmental consciousness has increased worldwide, leading to the development of eco-friendly materials to replace synthetic ones. Natural fibers are extracted from renewable resources at low cost. Their combination with synthetic polymers as reinforcement materials has been an important step forward in that direction. The sustainability and excellent physical and biological (e.g., biocompatibility, antimicrobial activity) properties of these biocomposites have extended their application to the biomedical field. This paper offers a detailed overview of the extraction and separation processes applied to natural fibers and their posterior chemical and physical modifications for biocomposite fabrication. Because of the requirements for biomedical device production, specialized biomolecules are currently being incorporated onto these biocomposites. From antibiotics to peptides and plant extracts, to name a few, this review explores their impact on the final biocomposite product, in light of their individual or combined effect, and analyzes the most recurrent strategies for biomolecule immobilization.
Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS
Authors: Chen Chuying , Peng Xuan , Chen Jinyin , Wan Chunpeng
Published: January 2020

The medicinal and edible plant, Ficus hirta Vahl. (also called hairy fig), is used for the treatment of constipation, inflammation, postpartum hypogalactia, tumors, and cancer. There is an urgent need for scientific evaluation to verify the pharmacological properties of F. hirta. Therefore, in vitro assays evaluated the antioxidant and antifungal activities of various solvent extracts of hairy fig fruits (HFF). HFF extracts had abundant antioxidant components for a significant amount of total phenolic (TPC) and flavonoid contents (TFC) (TPC from 17.75 ± 0.52 to 85.25 ± 1.72 mg gallic acid/g dw and TFC from 15.80 ± 0.59 to 144.22 ± 8.46 mg rutin/g dw, respectively). The ethyl acetate extract (EAE) and acetone extract (AE) of HFF demonstrated potent antioxidant activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50 values of 2.52 and 2.02 mg/mL, respectively) and ABTS radicals (IC50 values of 3.06 and 9.26 mg/mL, respectively). Moreover, the AE with a high TFC showed a prominent in vitro and in vivo antifungal activity against Penicillium italicum, causing citrus blue mold. Eighteen metabolites were identified or putatively identified from six HFF extracts. Current findings indicated that HFF extracts had significant antioxidant and antifungal activities and could potentially be used as an alternative agent for the preservation of agricultural products.
Grafting of Diazonium Salts on Surfaces: Application to Biosensors
Authors: Hetemi Dardan , Noël Vincent , Pinson Jean
Published: January 2020

This review is divided into two parts; the first one summarizes the main features of surface modification by diazonium salts with a focus on most recent advances, while the second part deals with diazonium-based biosensors including small molecules of biological interest, proteins, and nucleic acids.
Impaired Expression of Tetraspanin 32 (TSPAN32) in Memory T Cells of Patients with Multiple Sclerosis
Authors: Basile Maria Sofia , Mazzon Emanuela , Mangano Katia , Pennisi Manuela , Petralia Maria Cristina , Lombardo Salvo Danilo , Nicoletti Ferdinando , Fagone Paolo , Cavalli Eugenio
Published: January 2020

Tetraspanins are a conserved family of proteins involved in a number of biological processes. We have previously shown that Tetraspanin-32 (TSPAN32) is significantly downregulated upon activation of T helper cells via anti-CD3/CD28 stimulation. On the other hand, TSPAN32 is marginally modulated in activated Treg cells. A role for TSPAN32 in controlling the development of autoimmune responses is consistent with our observation that encephalitogenic T cells from myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) mice exhibit significantly lower levels of TSPAN32 as compared to naïve T cells. In the present study, by making use of ex vivo and in silico analysis, we aimed to better characterize the pathophysiological and diagnostic/prognostic role of TSPAN32 in T cell immunity and in multiple sclerosis (MS). We first show that TSPAN32 is significantly downregulated in memory T cells as compared to naïve T cells, and that it is further diminished upon ex vivo restimulation. Accordingly, following antigenic stimulation, myelin-specific memory T cells from MS patients showed significantly lower expression of TSPAN32 as compared to memory T cells from healthy donors (HD). The expression levels of TSPAN32 was significantly downregulated in peripheral blood mononuclear cells (PBMCs) from drug-naïve MS patients as compared to HD, irrespective of the disease state. Finally, when comparing patients undergoing early relapses in comparison to patients with longer stable disease, moderate but significantly lower levels of TSPAN32 expression were observed in PBMCs from the former group. Our data suggest a role for TSPAN32 in the immune responses underlying the pathophysiology of MS and represent a proof-of-concept for additional studies aiming at dissecting the eventual contribution of TSPAN32 in other autoimmune diseases and its possible use of TSPAN32 as a diagnostic factor and therapeutic target.
Simplified Design of Masonry Ring-Beams Reinforced by Flax Fibers for Existing Buildings Retrofitting
Authors: Guadagnuolo Mariateresa , Faella Giuseppe
Published: January 2020

Seismic events have repeatedly highlighted the vulnerability of existing masonry buildings. Seismic retrofitting is frequently focused on improving the connection between walls and roof for ensuring behavior able to resist loads from any horizontal direction. This paper deals with the design of masonry ring-beams made of clay bricks reinforced by natural fibers. Various solutions to ensure a masonry building box-behavior are possible, but this is a good combination of both static and conservation requirements, as it allows the use of bio-composites and grouts. It is a relevant possible alternative to the traditional reinforced concrete ring-beams, which are proven to be very ineffective under earthquakes. A simplified model for designing clay brick beams reinforced by flax fibers is provided, and a comparison with customary and traditional floor/roof masonry ring-beams is carried out.