bluuebunny/crossref_metadata_2025 huggingface dataset:
FireDA: A Domain Adaptation-Based Method for Forest Fire Recognition with Limited Labeled Scenarios
Authors: Yan Zhengjun , Zheng Xing , Li Wei , Wang Liming , Ding Peng , Zhang Ling , Yin Muyi , Wang Xiaowei
Published: September 2024

Vision-based forest fire detection systems have significantly advanced through Deep Learning (DL) applications. However, DL-based models typically require large-scale labeled datasets for effective training, where the quality of data annotation is crucial to their performance. To address challenges related to the quality and quantity of labeling, a domain adaptation-based approach called FireDA is proposed for forest fire recognition in scenarios with limited labels. Domain adaptation, a subfield of transfer learning, facilitates the transfer of knowledge from a labeled source domain to an unlabeled target domain. The construction of the source domain FBD is initiated, which includes three common fire scenarios: forest (F), brightness (B), and darkness (D), utilizing publicly available labeled data. Subsequently, a novel algorithm called Neighborhood Aggregation-based 2-Stage Domain Adaptation (NA2SDA) is proposed. This method integrates feature distribution alignment with target domain Proxy Classification Loss (PCL), leveraging a neighborhood aggregation mechanism and a memory bank designed for the unlabeled samples in the target domain. This mechanism calibrates the source classifier and generates more accurate pseudo-labels for the unlabeled sample. Consequently, based on these pseudo-labels, the Local Maximum Mean Discrepancy (LMMD) and the Proxy Classification Loss (PCL) are computed. To validate the efficacy of the proposed method, the publicly available forest fire dataset, FLAME, is employed as the target domain for constructing a transfer learning task. The results demonstrate that our method achieves performance comparable to the supervised Convolutional Neural Network (CNN)-based state-of-the-art (SOTA) method, without requiring access to labels from the FLAME training set. Therefore, our study presents a viable solution for forest fire recognition in scenarios with limited labeling and establishes a high-accuracy benchmark for future research.
Comparison Study of Hydrodynamic Characteristics in Different Swimming Modes of Carassius auratus
Authors: Zhang Ying , Jing Di , Huang Xiaoshuang , Chen Xinjun , Liu Bilin , Kong Xianghong
Published: September 2024

This study utilized particle image velocimetry (PIV) to analyze the kinematic and hydrodynamic characteristics of juvenile goldfish across three swimming modes: forward swimming, burst and coast, and turning. The results demonstrated that C-shaped turning exhibited the highest speed, enabling rapid and agile maneuvers for predator evasion. Meanwhile, forward swimming was optimal for sustained locomotion, and burst-and-coast swimming was suited for predatory behaviors. A vorticity analysis revealed that vorticity around the tail fin was the primary source of propulsive force, corroborating the correlation between vorticity magnitude and propulsion found in previous research. The findings emphasize the crucial role of the tail fin in swimming efficiency and performance. Future research should integrate ethology, biomechanics, and physiology to deepen the understanding of fish locomotion, potentially informing the design of efficient biomimetic underwater robots and contributing to fish conservation efforts.
Effect of Dietary Copper on Growth Performance, Antioxidant Capacity, and Immunity in Juvenile Largemouth Bass (Micropterus salmoides)
Authors: Kayiira John Cosmas , Mi Haifeng , Liang Hualiang , Ren Mingchun , Huang Dongyu , Zhang Lu , Teng Tao
Published: September 2024

This study evaluated the optimal dietary copper (Cu) levels and their effects on growth performance, body composition, and antioxidant capacity in juvenile largemouth bass (Micropterus salmoides). A total of 360 fish (initial average weight (1.67 ± 0.01 g) and initial average length (2.5 ± 0.2 cm)) were randomly assigned to 18 tanks, each containing 20 fish and six dietary Cu concentrations: 2.13 (control), 3.00, 3.66, 4.58, 4.64, and 5.72 mg/kg. The results indicated that fish receiving 3.00 mg/kg of Cu exhibited the best final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR), with a significantly reduced feed conversion ratio (FCR). While body composition (moisture, protein, lipid, and ash) remained consistent across groups, plasma total protein (TP) levels increased with Cu supplementation. Elevated triglycerides (TG) and albumin (ALB) were noted at 4.64 and 5.72 mg/kg, respectively, while glucose (GLU) levels decreased with an increase in dietary Cu. Antioxidant capacity, assessed via hepatic glutathione (GSH) and the activities of catalase (CAT), and showed significant improvements at 3.00 and 3.66 mg/kg Cu, while superoxide dismutase (SOD) showed the highest activity at a dietary Cu level of 5.72 mg/kg. Additionally, the expressions of tgf-β and tnf-α genes were significantly upregulated at a dietary Cu level of 5.72 mg/kg, while il-8 and il-10 genes were upregulated at dietary 3.66 mg/kg. The expression of nrf2 was significantly upregulated in response to a dietary Cu level of 3.66 mg/kg compared to the control group, and the expression of the keap1 gene was significantly upregulated in the fish fed with 5.72 mg/kg of dietary Cu. The results indicated that appropriate dietary supplementation could promote the growth performance and improve the antioxidant status the immunity of largemouth bass, and the optimal Cu requirement for FCR and SGR were approximately 3.10 mg/kg and 3.00 mg/kg, respectively.
Inhibitory Effects of Aqueous Ethanol Extracts of Poplar-Type Propolis on Advanced Glycation End Products and Protein Oxidation
Authors: Wang Guangxin , Zhang Yu , Qiao Jiangtao , El-Seedi Hesham R. , Kong Lingjie , Zhang Hongcheng
Published: September 2024

(1) Background: The non-enzymatic glycation of proteins is a significant contributor to the formation of advanced glycation end products (AGEs) and intermediates that are responsible for diabetic complications. It is imperative to explore effective inhibitors to prevent protein glycation. (2) Methods: This study aimed to investigate the inhibitory potential of various aqueous ethanol extracts of poplar-type propolis on AGEs and oxidative modifications in bovine serum albumin (BSA)-glucose and BSA-methylglyoxal models. (3) Results: The results revealed that these propolis extracts exhibited significant effectiveness in inhibiting the formation of total AGEs, pentosidine, and Nε-carboxymethyllysine (CML). Furthermore, the investigation discovered that these propolis extracts can effectively inhibit oxidative modification, based on measuring the levels of carbonyl and thiol groups and analyzing tryptophan fluorescence quenching. Notably, 75% ethanol extracts of propolis (EEP) exhibited the highest inhibitory activity, surpassing the chemical inhibitor aminoguanidine (AG). (4) Conclusions: The remarkable anti-glycation potency of aqueous ethanol extracts of poplar-type propolis can be attributed to their elevated contents of phenolic compounds, especially abundant flavonoids, which inhibit the formation of AGEs by scavenging free radicals, decreasing the levels of reactive oxygen species (ROS), and capturing reactive carbonyl species (RCS) in the protein glycation process. Our results indicate that poplar-type propolis may be a potential AGE inhibitor and could be used to develop functional foods and nutraceuticals to prevent diabetic complications.
Comparative Analysis of XGB, CNN, and ResNet Models for Predicting Moisture Content in Porphyra yezoensis Using Near-Infrared Spectroscopy
Authors: Zhang Wenwen , Pan Mingxuan , Wang Peng , Xue Jiao , Zhou Xinghu , Sun Wenke , Hu Yadong , Shen Zhaopeng
Published: September 2024

This study explored the performance and reliability of three predictive models—extreme gradient boosting (XGB), convolutional neural network (CNN), and residual neural network (ResNet)—for determining the moisture content in Porphyra yezoensis using near-infrared (NIR) spectroscopy. We meticulously selected 380 samples from various sources to ensure a comprehensive dataset, which was then divided into training (300 samples) and test sets (80 samples). The models were evaluated based on prediction accuracy and stability, employing genetic algorithms (GA) and partial least squares (PLS) for wavelength selection to enhance the interpretability of feature extraction outcomes. The results demonstrated that the XGB model excelled with a determination coefficient (R2) of 0.979, a root mean square error of prediction (RMSEP) of 0.004, and a high ratio of performance to deviation (RPD) of 4.849, outperforming both CNN and ResNet models. A Gaussian process regression (GPR) was employed for uncertainty assessment, reinforcing the reliability of our models. Considering the XGB model’s high accuracy and stability, its implementation in industrial settings for quality assurance is recommended, particularly in the food industry where rapid and non-destructive moisture content analysis is essential. This approach facilitates a more efficient process for determining moisture content, thereby enhancing product quality and safety.
Carob (Ceratonia siliqua) Flour as Source of Bioactive Compounds: Production, Characterization and Nutraceutical Value
Authors: Benito-Vázquez Iván , Garrido-Romero Manuel , Hontoria-Caballo Gema , García-García Carlos , Díez-Municio Marina , Moreno F. Javier
Published: September 2024

Carob (Ceratonia siliqua) seeds are rich in diverse bioactive compounds, including galactomannan, β-sitosterol, unsaturated fatty acids and proteins with bioactive peptides in their sequence. This study delineates the compositional characterization of six carob seed flour types derived from different production processes, providing valuable insights for designing tailored nutraceutical products based on desired bioactive compound profiles. Our analysis indicated that a higher purity of galactomannan resulted in a greater mannose/galactose ratio, which increased the linearity of the galactomannan polymer and could enhance interchain interaction, thereby increasing aggregation capacity. A higher viscosity could potentially increase the capacity of galactomannan to create satiety and lower cholesterol levels. Among the different tested flours, those whose main compound was the endosperm were optimal for containing high galactomannan content, whereas those derived from the germ were ideal for having high concentrations of fatty acids (i.e., oleic and linoleic acids) and β-sitosterol. The presence of these lipids in carob flours could offer cardiovascular and metabolic health benefits, contributing synergistically. Additionally, flours that contain the germ have beneficial peptides included in proteins like glycinin and conglutin with potential anticholesterolemic and antidiabetic properties. This work provides different methods for obtaining carob flours rich in bioactive compounds, offering the nutraceutical industry a framework to select the best option for industrial-scale production.
Slovenia’s Food-Based Dietary Guidelines 2024: Eating for Health and the Planet
Authors: Fras Zlatko , Jug Borut , Jakše Boštjan , Kreft Samo , Mikec Nina , Malek Žiga , Bavec Martina , Vovk Ana , Frelih-Larsen Ana , Fidler Mis Nataša
Published: September 2024

The dietary guidelines of Slovenia, ‘12 Steps to Healthy Eating’, were first published in 2000 and revised in 2011. The ‘Food Guide Pyramid’ was initially published in 2000 and subsequently revised in 2015. ‘The Healthy Plate’ was first introduced in 2007. In February 2023, the Slovenian Strategic Council for Nutrition proposed new Food-Based Dietary Guidelines (FBDGs) that integrate both health and environmental considerations. In September 2023, the creation of new FBDGs was included in the Action Plan for implementing the Resolution on the National Program on Nutrition and Physical Activity for Health 2015–2025. In October 2023, the Ministry of Health of Slovenia appointed the core working group of 10 multidisciplinary experts from fields such as nutrition, food science medicine, public health, environment, pharmacy, and agriculture led by Prof. Dr. Nataša Fidler Mis, who drafted the guidelines. In February 2024, the World Health Organization Regional Office for Europe organized a virtual international workshop to assist Slovenia in developing food-based dietary guidelines. In May 2024, an international expert meeting was organized by the Ministry of Health of Slovenia, the Ministry of the Environment, Climate, and Energy, and the National Institute of Public Health of Slovenia to present the first scientific draft of the SLO FBDG for external international peer review. The meeting included lectures from world-leading experts to present healthy diets from sustainable food systems, integrate climate and sustainability aspects into the new SLO FBDG, discuss the findings with the Slovenian core working group, extended working group of the SLO FBDG, and invited experts. The final version of SLO FBDG is expected to be released by the end of 2024.
A Novel Fractional Model and Its Application in Network Security Situation Assessment
Authors: Huang Ruixiao , Pu Yifei
Published: September 2024

The evaluation process of the Fractional Order Model is as follows. To address the commonly observed issue of low accuracy in traditional situational assessment methods, a novel evaluation algorithm model, the fractional-order BP neural network optimized by the chaotic sparrow search algorithm (TESA-FBP), is proposed. The fractional-order BP neural network, by incorporating fractional calculus, demonstrates enhanced dynamic response characteristics and historical dependency, showing exceptional potential for handling complex nonlinear problems, particularly in the field of network security situational awareness. However, the performance of this network is highly dependent on the precise selection of network parameters, including the fractional order and initial values of the weights. Traditional optimization methods often suffer from slow convergence, a tendency to be trapped in local optima, and insufficient optimization accuracy, which significantly limits the practical effectiveness of the fractional-order BP neural network. By introducing cubic chaotic mapping to generate an initial population with high randomness and global coverage capability, the exploration ability of the sparrow search algorithm in the search space is effectively enhanced, reducing the risk of falling into local optima. Additionally, the Estimation of Distribution Algorithm (EDA) constructs a probabilistic model to guide the population toward the globally optimal region, further improving the efficiency and accuracy of the search process. The organic combination of these three approaches not only leverages their respective strengths, but also significantly improves the training performance of the fractional-order BP neural network in complex environments, enhancing its generalization ability and stability. Ultimately, in the network security situational awareness system, this integration markedly enhances the prediction accuracy and response speed.
Factors Influencing Elderly Consumers’ Preferences for Edible Gels: Insights from Slovakia
Authors: Korčok Melina , Veverka Miroslav , Nakonechna Kristina , Škrípová Simona , Vietoris Vladimir
Published: September 2024

As dietary needs shift with the growing and aging population, there is a demand for food products that meet nutritional, safety, and tribological requirements while being cost-effective. Seniors must be given significant consideration in new product development. This study examines consumer preferences for arabinogalactan (AG) and beta-glucan (BG) hydrogels with vanilla and coffee-biscuit flavors, using consumer tests (N = 80) and an online survey (N = 852). It focuses on the gels’ physical properties, such as texture and viscosity, and their impact on sensory perception. The use of two different gel-forming polysaccharides, each with a unique sensory profile, was observed to affect the sensory properties of the resulting gels and subsequently influence product acceptance. This study analyzed preferences across three age groups: young (18–39 years), middle-aged (40–59 years), and older adults (60+ years). The results showed that seniors preferred AG-based gels. Significant attributes such as the intensity of flavor and bitter taste influenced the overall liking of the gels. Texture also notably impacted preferences. The survey findings revealed statistically significant (p < 0.05) differences in preferences between older adults and younger age groups. Tailoring product development and marketing strategies based on age and sensory preferences could enhance consumer acceptance of edible gels.
Characteristics and Trends of Workplace Violence towards Frontline Health Workers under Comprehensive Interventions in a Chinese Infectious Disease Hospital
Authors: Huang Yiming , Zhang Min , He Chuning , Wang Fuyuan , Liu Yujie , Wu Jing , Luo Qianqian , Chen Na , Tang Yuting
Published: September 2024

Objectives: This study investigated workplace violence (WPV) toward frontline health workers under comprehensive interventions to improve the occupational safety and health management system in a Chinese infectious disease hospital. Methods: The risk assessment of WPV using an international questionnaire was conducted in 2018 and 2021 to compare the perceived levels of exposure to WPV and intervention measures before and after the intensification of anti-violence measures in the hospital context. Additionally, qualitative data were collected in 2021 through semi-structured and unstructured interviews, providing complementary information about WPV toward frontline health workers (HWs). Results: After establishing the occupational safety and health management system (OSHMS), the total incidence rate of WPV decreased from 60.90% in 2018 to 34.44% in 2021. Psychological violence declined significantly from 60.90% in 2018 to 33.89% in 2021. The endorsement of precautionary measures increased significantly from 2018 to 2021, including patient screening recognition, patient protocol, shift or rota changes, etc. A thematic analysis of several subthemes shows that HWs had an in-depth understanding of WPV, recognizing its multifaceted consequences in the context of complex risk factors. Conclusions: This study demonstrates a significant decrease in WPV, psychological violence, verbal abuse, bullying/mobbing, and ethnic discrimination after implementing the comprehensive OSHMS.
Overexpression of the EuSIP5 Gene to Improve Drought Resistance in Tobacco
Authors: Lin Yueling , Chen Xi , Zhao Degang , Li Chao
Published: September 2024

Soluble inorganic pyrophosphatase (s-PPase), a pyrophosphate hydrolase, is crucial for various physiological processes including plant growth and development, metabolic functions, and responses to abiotic stresses. However, research on s-PPase in woody plants is limited. To investigate the potential role of soluble inorganic pyrophosphatase in Eucommia ulmoides Oliver (E. ulmoides) in drought stress, the E. ulmoides soluble inorganic pyrophosphatase 5 (EuSIP5) cDNA sequence was amplified via RT-PCR. A bioinformatic analysis suggested that EuSIP5 may be an unstable amphipathic protein predominantly localized in the cytoplasm. In E. ulmoides, the highest expression of the EuSIP5 gene was detected in the leaves and pericarp of male plants from April to October, and in the leaves in July and September. Under drought conditions, the expression of EuSIP5 in E. ulmoides leaves was significantly greater than that in the control. An overexpression vector containing EuSIP5 was constructed and introduced into Nicotiana tabacum L. cv. Xanthi (N. tabacum L.). Compared with that in wild-type (WT) plants, wilting in N. tabacum L. EuSIP5-overexpressing (OE) plants was delayed by 4 days under drought stress. Additionally, the expression levels of the drought-related genes DET2, CYP85A1, P5CS, ERF1, F-box, and NCED1 were elevated in the leaves of transgenic N. tabacum L. Moreover, the activities of the protective enzymes peroxidase, superoxide dismutase, and catalase were significantly greater, whereas the malondialdehyde content was lower in the transgenic plants than in the WT plants. These findings suggest that the introduction of the EuSIP5 gene into N. tabacum L. enhances drought-related gene expression, increases antioxidant capacity, and reduces oxidative stress damage, thereby improving drought resistance.
Prolonging Cyclamen Flower Vase Life via 8-HQS and AgNO3 Treatments in a Controlled Release System
Authors: Mollaei Samane , Mirdehghan Seyed Hossein , Profico Cosimo M. , Nicola Silvana , Caldera Fabrizio , Trotta Francesco , Devecchi Marco , Cecone Claudio
Published: September 2024

The current study applied electrospun fibers containing 8-hydroxyquinoline-5-sulfonic acid (8-HQS) (100, 150, and 200 mg L−1) and silver nitrate (AgNO3) (15 and 20 mg L−1) to enhance the longevity and qualitative parameters of two cyclamen cultivars. The results indicated that the vase life of the flowers treated with 20 mg L−1 AgNO3 and 200 mg L−1 8-HQS was higher than that of the other treatments (16 days). Cyclamens treated with 8-HQS (100 mg L−1) + AgNO3 (15 mg L−1) and 8-HQS (100 mg L−1) + AgNO3 (20 mg L−1) had lower polyphenol oxidase (PPO) activity than the control group. Also, it was observed that the higher phenylalanine ammonia-lyase (PAL) enzyme activity of cyclamens in the 8-HQS (150 mg L−1) + AgNO3 (15 mg L−1) treatment was longer than that of the other treatments and control groups. Treatment with 100 mg L−1 8-HQS and 20 mg L−1 AgNO3 showed higher peroxidase (POD) activity than the different treatments. This research indicated that adding AgNO3 and 8-HQS to electrospun fibers is a promising method for enhancing the longevity and maintaining the quality of these cut flowers.
Basic Affective Systems and Sex Differences in the Relationship between Anger and Fear
Authors: Manfredi Paola
Published: September 2024

Background: The possible interactions between anger and fear have not been widely explored in the psychological literature. Fear and anger are currently beginning to be studied by looking at their interrelationships, rather than seeing them as simply opposing emotions. Furthermore, there is a tendency to think that anger is more typical of men and fear of women. Our contribution proposes a particular perspective of affective neuroscience. The objectives of the study are as follows: (1) to assess possible differences in affective systems, and states and traits of anger in relation to biological sex; (2) to assess correlations between ANGER, FEAR, and SADNESS, as well as state and trait anger in both a female and male sample; (3) to assess possible differences in basic affective systems in relation to different levels of ANGER, FEAR, and SADNESS, state and trait anger, in female and male samples. Methods: A non-clinical sample of 339 females and 99 males completed the ANPS 3.1 to assess basic affective states and the STAXI-2 to assess anger states and traits. Results: No significant differences were found for ANGER and FEAR scores and for state and trait scores between the male and female samples. Clear correlations emerged (p < 0.01) between SADNESS and FEAR in both the female and male samples. Among the differences that emerged in the affective systems, we emphasise that in the female group, the highest scores on the SEEKING and PLAY scales are expressed by the group of women who have the lowest scores in FEAR; PLAY and CARE also vary in relation to different scores in SADNESS. Discussion: Given the importance of the SEEKING and PLAY variables, it is of paramount importance to monitor the environmental and relational situations to guarantee that women, too, are provided with the conditions of safety and protection that are prerequisites for their well-being and the positive expression of their resources.
Novel Insights into Diabetic Kidney Disease
Authors: Młynarska Ewelina , Buławska Dominika , Czarnik Witold , Hajdys Joanna , Majchrowicz Gabriela , Prusinowski Filip , Stabrawa Magdalena , Rysz Jacek , Franczyk Beata
Published: September 2024

Diabetic kidney disease (DKD) is a major complication of diabetes mellitus (DM), affecting over one-third of type 1 and nearly half of type 2 diabetes patients. As the leading cause of end-stage renal disease (ESRD) globally, DKD develops through a complex interplay of chronic hyperglycemia, oxidative stress, and inflammation. Early detection is crucial, with diagnosis based on persistent albuminuria and reduced estimated glomerular filtration rate (eGFR). Treatment strategies emphasize comprehensive management, including glycemic control, blood pressure regulation, and the use of nephroprotective agents such as angiotensin-converting enzyme (ACE) inhibitors, angiotensin II receptor blockers (ARBs), sodium-glucose cotransporter-2 (SGLT2) inhibitors, and glucagon-like peptide-1 (GLP-1) receptor agonists. Ongoing research explores novel therapies targeting molecular pathways and non-coding RNAs. Preventive measures focus on rigorous control of hyperglycemia and hypertension, aiming to mitigate disease progression. Despite therapeutic advances, DKD remains a leading cause of ESRD, highlighting the need for continued research to identify new biomarkers and innovative treatments.
Electroacupuncture Relieves Neuropathic Pain via Adenosine 3 Receptor Activation in the Spinal Cord Dorsal Horn of Mice
Authors: Kiani Faisal Ayub , Li Hao , Nan Sha , Li Qiuhua , Lei Qianghui , Yin Ruiling , Cao Shiya , Ding Mingxing , Ding Yi
Published: September 2024

Neuropathic pain (NPP) is a devastating and unbearable painful condition. As prevailing treatment strategies have failed to mitigate its complications, there remains a demand for effective therapies. Electroacupuncture (EA) has proved a potent remedial strategy in NPP management in humans and mammals. However, past studies have investigated the underlying mechanism of the analgesic effects of EA on NPP, focusing primarily on adenosine receptors in peripheral tissues. Herein, we elucidate the role of the adenosine (Adora-3) signaling pathway in mediating pain relief through EA in the central nervous system, which is obscure in the literature and needs exploration. Specific pathogen-free (SPF) male adult mice (C57BL/6 J) were utilized to investigate the effect of EA on adenosine metabolism (CD73, ADA) and its receptor activation (Adora-3), as potential mechanisms to mitigate NPP in the central nervous system. NPP was induced via spared nerve injury (SNI). EA treatment was administered seven times post-SNI surgery, and lumber (L4–L6) spinal cord was collected to determine the molecular expression of mRNA and protein levels. In the spinal cord of mice, following EA application, the expression results revealed that EA upregulated (p < 0.05) Adora-3 and CD73 by inhibiting ADA expression. In addition, EA triggered the release of adenosine (ADO), which modulated the nociceptive responses and enhanced neuronal activation. Meanwhile, the interplay between ADO levels and EA-induced antinociception, using an Adora-3 agonist and antagonist, showed that the Adora-3 agonist IB-MECA significantly increased (p < 0.05) nociceptive thresholds and expression levels. In contrast, the antagonist MRS1523 exacerbated neuropathic pain. Furthermore, an upregulated effect of EA on Adora-3 expression was inferred when the Adora-3 antagonist was administered, and the EA treatment increased the fluorescent intensity of Adora-3 in the spinal cord. Taken together, EA effectively modulates NPP by regulating the Adora-3 signaling pathway under induced pain conditions. These findings enhance our understanding of NPP management and offer potential avenues for innovative therapeutic interventions.
Genome Instability Induced by Topoisomerase Misfunction
Authors: Nitiss Karin C. , Bandak Afif , Berger James M. , Nitiss John L.
Published: September 2024

Topoisomerases alter DNA topology by making transient DNA strand breaks (DSBs) in DNA. The DNA cleavage reaction mechanism includes the formation of a reversible protein/DNA complex that allows rapid resealing of the transient break. This mechanism allows changes in DNA topology with minimal risks of persistent DNA damage. Nonetheless, small molecules, alternate DNA structures, or mutations in topoisomerase proteins can impede the resealing of the transient breaks, leading to genome instability and potentially cell death. The consequences of high levels of enzyme/DNA adducts differ for type I and type II topoisomerases. Top1 action on DNA containing ribonucleotides leads to 2–5 nucleotide deletions in repeated sequences, while mutant Top1 enzymes can generate large deletions. By contrast, small molecules that target Top2, or mutant Top2 enzymes with elevated levels of cleavage lead to small de novo duplications. Both Top1 and Top2 have the potential to generate large rearrangements and translocations. Thus, genome instability due to topoisomerase mis-function is a potential pathogenic mechanism especially leading to oncogenic progression. Recent studies support the potential roles of topoisomerases in genetic changes in cancer cells, highlighting the need to understand how cells limit genome instability induced by topoisomerases. This review highlights recent studies that bear on these questions.
Reposition: Focalizing β-Alanine Metabolism and the Anti-Inflammatory Effects of Its Metabolite Based on Multi-Omics Datasets
Authors: Luo Wenjun , Zhang Haijun , Zhang Hao , Xu Yixi , Liu Xiao , Xu Shijun , Wang Ping
Published: September 2024

The incorporation of multi-omics data methodologies facilitates the concurrent examination of proteins, metabolites, and genes associated with inflammation, thereby leveraging multi-dimensional biological data to achieve a comprehensive understanding of the complexities involved in the progression of inflammation. Inspired by ensemble learning principles, we implemented ID normalization preprocessing, categorical sampling homogenization, and pathway enrichment across each sample matrix derived from multi-omics datasets available in the literature, directing our focus on inflammation-related targets within lipopolysaccharide (LPS)-stimulated RAW264.7 cells towards β-alanine metabolism. Additionally, through the use of LPS-treated RAW264.7 cells, we tentatively validated the anti-inflammatory properties of the metabolite Ureidopropionic acid, originating from β-alanine metabolism, by evaluating cell viability, nitric oxide production levels, and mRNA expression of inflammatory biomarkers. In conclusion, our research represents the first instance of an integrated analysis of multi-omics datasets pertaining to LPS-stimulated RAW264.7 cells as documented in the literature, underscoring the pivotal role of β-alanine metabolism in cellular inflammation and successfully identifying Ureidopropionic acid as a novel anti-inflammatory compound. Moreover, the findings from database predictions and molecular docking studies indicated that the inflammatory-related pathways and proteins may serve as potential mechanistic targets for Ureidopropionic acid.
Ultrasonic-Assisted Conversion of Micrometer-Sized BiI3 into BiOI Nanoflakes for Photocatalytic Applications
Authors: Das Tushar Kanti , Jesionek Marcin , Mistewicz Krystian , Nowacki Bartłomiej , Kępińska Mirosława , Zubko Maciej , Godzierz Marcin , Gawron Anna
Published: September 2024

This work describes a novel method for converting bismuth triiodide (BiI3) microplates into bismuth oxyiodide (BiOI) nanoflakes under ultrasonic irradiation. To produce BiOI nanoflakes with a high yield and high purity, the conversion process was carefully adjusted. Rapid reaction kinetics and increased mass transfer are benefits of the ultrasonic-assisted approach that result in well-defined converted BiOI nanostructures with superior characteristics. The produced BiOI nanoflakes were examined utilizing a range of analytical methods, such as Transmission Electron Microscopy (TEM), scanning electron microscopy (SEM) and X-ray diffraction (XRD). The progress in the ultrasonic conversion process with time was monitored through diffuse reflectance spectroscopy (DRS). The outcomes demonstrated the effective conversion of BiI3 microplates into crystalline, homogeneous, high-surface-area BiOI nanoflakes. Additionally, the degradation of organic dyes (methylene blue) under ultraviolet (UV) light irradiation was used to assess the photocatalytic efficacy of the produced BiOI nanoflakes. Because of their distinct morphology and electrical structure, the BiOI nanoflakes remarkably demonstrated remarkable photocatalytic activity, outperforming traditional photocatalysts. The ability of BiOI nanoflakes to effectively separate and utilize visible light photons makes them a viable option for environmental remediation applications. This work not only shows the promise of BiOI nanoflakes for sustainable photocatalytic applications but also demonstrates a simple and scalable approach to their manufacturing. The knowledge gathered from this work opens up new avenues for investigating ultrasonic-assisted techniques for creating sophisticated nanomaterials with customized characteristics for a range of technological uses.
Microbial Community Structure and Metabolic Function in the Venom Glands of the Predatory Stink Bug, Picromerus lewisi (Hemiptera: Pentatomidae)
Authors: Li Jinmeng , Tian Xu , Hsiang Tom , Yang Yuting , Shi Caihua , Wang Hancheng , Li Wenhong
Published: September 2024

The predatory stink bug, Picromerus lewisi (Hemiptera: Pentatomidae), is an important and valuable natural enemy of insect pests in their ecosystems. While insects are known to harbor symbiotic microorganisms, and these microbial symbionts play a crucial role in various aspects of the host’s biology, there is a paucity of knowledge regarding the microbiota present in the venom glands of P. lewisi. This study investigated the venom glands of adult bugs using both traditional in vitro isolation and cultural methods, as well as Illumina high-throughput sequencing technology. Additionally, the carbon metabolism of the venom gland’s microorganisms was analyzed using Biolog ECO metabolic phenotyping technology. The results showed 10 different culturable bacteria where the dominant ones were Enterococcus spp. and Lactococcus lactis. With high-throughput sequencing, the main bacterial phyla in the microbial community of the venom glands of P. lewisi were Proteobacteria (78.1%) and Firmicutes (20.3%), with the dominant bacterial genera being Wolbachia, Enterococcus, Serratia, and Lactococcus. At the fungal community level, Ascomycota accounted for the largest proportion (64.1%), followed by Basidiomycota (27.6%), with Vishniacozyma, Cladosporium, Papiliotrema, Penicillium, Fusarium, and Aspergillus as the most highly represented fungal genera. The bacterial and fungal community structure of the venom glands of P. lewisi exhibited high species richness and diversity, along with a strong metabolism of 22 carbon sources. Functional prediction indicated that the primary dominant function of P. lewisi venom-gland bacteria was metabolism. The dominant eco-functional groups of the fungal community included undefined saprotroph, fungal parasite–undefined saprotroph, unassigned, endophyte–plant pathogen, plant pathogen–soil saprotroph–wood saprotroph, animal pathogen–endophyte–plant pathogen–wood saprotroph, plant pathogen, and animal pathogen–endophyte–epiphyte–plant pathogen–undefined saprotroph. These results provide a comprehensive characterization of the venom-gland microbiota of P. lewisi and demonstrate the stability (over one week) of the microbial community within the venom glands. This study represents the first report on the characterization of microbial composition from the venom glands of captive-reared P. lewisi individuals. The insights gained from this study are invaluable for future investigations into P. lewisi’s development and the possible interactions between P. lewisi’s microbiota and some Lepidopteran pests.
Measurement of the Direct Impact of Hematophagous Flies on Feeder Cattle: An Unexpectedly High Potential Economic Impact
Authors: Boonsaen Phoompong , Nevot Adèle , Onju Sathaporn , Fossaert Clément , Chalermwong Piangjai , Thaisungnoen Kornkanok , Lucas Antoine , Thévenon Sophie , Masmeatathip Roungthip , Jittapalapong Sathaporn , Desquesnes Marc
Published: September 2024

In addition to blood pathogen transmission, insects of the order Diptera affect livestock through visual and contact harassment; blood-feeders are responsible for painful bites and blood despoliation, generating behavioral modifications, anemia, and production losses. Knowledge of their economic impact is a basis for cost-effective control. Here, we measured the global impact of diptera insects by comparing two batches of six feeder cattle, one in the open air and the other protected by a mosquito net. The analytical data were insect density in the open air and, for feeder cattle, tail flick counts, hematocrit values (Ht), feed intake, feed conversion ratio (FCR), and live body weight gain (LBWG). Over a period of five months, the results showed significant losses in the LBWG of cattle exposed to insects, estimated at 8.0 ± 1.5 kg/month [2.7; 13.3], with a total loss reaching 40.0 ± 5.5 kg/head. Main diurnal insects were Stomoxys spp. and Musca crassirostris. There was a strong correlation between fly density and diurnal tail flicks. Night trapping and tail flicks showed a potentially important role of mosquitoes to be further explored. The Ht levels of exposed animals were 3–4% lower than those of controls. FCRs indicated that exposed animals needed 33% more dry matter intake/kg of LBWG. An economic assessment showed that dipterans were responsible for a 10–11% loss in LBWG during the main growing period of feeder cattle (10–15 months). A feedlot of 100 calves would register a total loss of USD 16,000 within 5 months, which appears to be an unexpectedly huge loss caused by dipterans. Investing part of this money into fly control would probably be beneficial.
Genome-Wide Search for Gene Mutations Likely Conferring Insecticide Resistance in the Common Bed Bug, Cimex lectularius
Authors: Toga Kouhei , Kimoto Fumiko , Fujii Hiroki , Bono Hidemasa
Published: September 2024

Insecticide resistance in the bed bug Cimex lectularius is poorly understood due to the lack of genome sequences for resistant strains. In Japan, we identified a resistant strain of C. lectularius that exhibits a higher pyrethroid resistance ratio compared to many previously discovered strains. We sequenced the genomes of the pyrethroid-resistant and susceptible strains using long-read sequencing, resulting in the construction of highly contiguous genomes (N50 of the resistant strain: 2.1 Mb and N50 of the susceptible strain: 1.5 Mb). Gene prediction was performed by BRAKER3, and the functional annotation was performed by the Fanflow4insects workflow. Next, we compared their amino acid sequences to identify gene mutations, identifying 729 mutated transcripts that were specific to the resistant strain. Among them, those defined previously as resistance genes were included. Additionally, enrichment analysis implicated DNA damage response, cell cycle regulation, insulin metabolism, and lysosomes in the development of pyrethroid resistance. Genome editing of these genes can provide insights into the evolution and mechanisms of insecticide resistance. This study expanded the target genes to monitor allele distribution and frequency changes, which will likely contribute to the assessment of resistance levels. These findings highlight the potential of genome-wide approaches to understand insecticide resistance in bed bugs.
Sutureless Aortic Prosthesis Valves versus Transcatheter Aortic Valve Implantation in Intermediate Risk Patients with Severe Aortic Stenosis: A Literature Review
Authors: Asta Laura , Sbrigata Adriana , Pisano Calogera
Published: September 2024

Aortic stenosis remains the most frequently occurring valvular pathology in the elderly population of Western countries. According to the latest guidelines, the therapeutic choice of aortic stenosis depends on the age of the patient (<75 years or >75 years) and the risk class (STS-Prom/Euroscore II < o >4%). Therefore, if the surgical indication is clear in young and low-risk patients and percutaneous treatment is the gold standard in older and high-risk patients, the therapeutic choice is still debated in intermediate-risk patients. In this group of patients, aortic valve stenosis treatment depends on the patient’s global evaluation, the experience of the center, and, no less importantly, the patient’s will. Two main therapeutic options are debated: surgical aortic valve replacement with sutureless prosthesis versus transcatheter aortic valve implantation. In addition, the progressive development of mininvasive techniques for aortic valve surgery (right-anterior minithoracotomy) has also reduced the peri- and post-operative risk in this group of patients. The purpose of this review is to compare sutureless aortic valve replacement (SuAVR) versus TAVI in intermediate-risk patients with severe aortic stenosis.
Lactate/Pyruvate Ratio as an Early Predictor of Mortality in Patients with Sepsis: A Cohort Study
Authors: Cantu-Rodriguez Olga G. , Hawing-Zarate Jose A. , Dorsey-Trevino Edgar G. , Hernandez-Barajas David , Villalobos-Gutierrez Leonel E. , Jaime-Perez Jose Carlos , Mancias-Guerra Consuelo , Gonzalez-Llano Oscar , Gonzalez-Cantu Graciela A. , Gomez-Almaguer David , Gutierrez-Aguirre Cesar H.
Published: September 2024

Background: The lactate/pyruvate (LP) ratio has been studied as an alternative to serum lactate to determine clinical prognosis. Despite its clinical utility, there is a paucity of evidence evaluating the role of the L/P ratio in patients with sepsis. Methods: We assessed the clinical utility of the L/P ratio in patients with sepsis. The L/P ratio was measured at baseline, 4 and 8 h after admission. Our primary outcome was to determine the prognostic utility of the L/P ratio on the 15-day mortality risk. Our secondary outcomes were to compare the L/P ratio across time and its prognostic utility against standard risk calculators such as APACHE-II and SOFA scores. Results: We had a total of 80 patients, with 18 (22.5%) survivors and 62 (77.5%) non-survivors. While we found that patients having higher L/P ratios at 8 h had an increased 30-mortality risk (OR 1.08, 95% CI 1.02–1.18), the model’s performance showed no difference when compared to other measurements of the L/P ratio that showed no association with mortality (p-value: 0.45). For our secondary outcome, we found that the APACHE-II and SOFA scores have better performance and predictability than the L/P ratio (AUC 0.83 and AUC 0.80, respectively), but showed no association with mortality (OR 1.07, 95% CI 1.01–1.17 and OR 1.08, 95% CI 1.02–1.18). Conclusions: Based on our findings, the L/P ratio appears to function more effectively as an early predictor of mortality when used as an adjuvant biomarker with other clinical parameters.
Reduced Rate of Anemia after Transcatheter Aortic Valve Replacement
Authors: Eitan Amnon , Sliman Hussein , Zafrir Barak , Zissman Keren , Flugelman Moshe Y. , Jaffe Ronen
Published: September 2024

Background/Objectives: We sought to evaluate changes in hemoglobin level and renal function in patients 5–12 months after transcatheter aortic valve replacement (TAVR), and to examine possible relationships between these changes. Anemia is common in older people with severe aortic stenosis (AS). The two most common etiologies for anemia in this population are iron deficiency due to gastrointestinal blood loss and renal failure. Angiodysplasia in the gastrointestinal system is a feature of AS syndrome. Methods: We collected clinical data, including hemoglobin level and renal function before and 5–12 months after TAVR in 315 consecutive patients. To examine whether calculated clinical predictors such as EuroScore 2 are associated with the persistence of anemia after TAVR, we performed multivariable correlation analysis with post-TAVR anemia as the dependent variable. Results: The mean hemoglobin level increased significantly (from 11.76 to 12.16 g/dL, p < 0.0001) 5–12 months after TAVR, and the number of patients with anemia decreased significantly (from 67.5% to 53.9%, p < 0.0001). At 5–12 months following TAVR, a small reduction in estimated glomerular filtration rate was observed (from 60.05 ± 24.1 to 58.30 ± 24.50 mL/min, p = 0.024). The multivariable correlation analysis did not identify clinical predictors of persistent anemia. Conclusions: A significant increase in hemoglobin was observed 5–12 months after TAVR, despite a reduction in renal function. Our findings imply that gastrointestinal blood loss, which occurs in patients with severe AS, is significantly reduced following TAVR.
Positive Cutibacterium acnes Intervertebral Discs Are Not Associated with Subsidence Following Anterior Cervical Discectomy and Fusion at 3 or 6 Months
Authors: Camacho Jael , Carbone Jake , Suresh Rohan I. , Khanna Shivam , Ye Ivan B. , Thomson Alexandra E. , Bruckner Jacob , Gopinath Rohan , McGowan Shane , O’Hara Nathan , Bivona Louis J. , Jauregui Julio J. , Cavanaugh Daniel L. , Koh Eugene Y. , Ludwig Steven C.
Published: September 2024

Background/Objectives: Cutibacterium acnes (C. acnes), formerly known as Propionibacterium acnes (P. acnes), is an anaerobic, low-virulent bacterium that has been associated with postoperative infections of the shoulder, knee, and cervical spine. Recent studies have highlighted an association between C. acnes and the development of degenerative disc disease (DDD). The aim of this study is to ascertain whether C. acnes increases the risk of subsidence following anterior cervical discectomy and fusion (ACDF). Methods: After IRB approval, consecutive patients undergoing elective ACDF for DDD from 2017 to 2018 were enrolled in this prospective cohort study. Intervertebral disc samples were taken at each affected level and cultured. A total of 66 patients with radiographic follow-ups were included in the final analysis. The extent of subsidence and cervical lordosis was determined immediately postoperatively and at the 3- and 6-month follow-ups. Results: No significant difference in subsidence was observed at 3 months (p = 0.07) or 6 months (p = 0.11) between culture-positive and -negative cohorts. Additionally, there was no significant difference detected in the change in cervical lordosis observed at 3 months (p = 0.16) or 6 months (p = 0.27) between culture-positive and -negative cohorts. For the most inferiorly fused segment, there was a significant difference in subsidence observed at 3 months (1.5 mm, 95% CI: 0.2–2.7 mm, p = 0.02) but not at 6 months (p = 0.17). Conclusions: Intervertebral discs with a positive C. acnes culture were not associated with greater levels of subsidence at 3 or 6 months following ACDF for DDD. Further research is necessary to endorse these results and to gauge the clinical significance of C. acnes infection.