bluuebunny/crossref_metadata_2025 huggingface dataset:
From Talent Identification to Novo Basquete Brasil (NBB): Multifactorial Analysis of the Career Progression in Youth Brazilian Elite Basketball
Authors: Ribeiro Junior Dilson B. , Werneck Francisco Z. , Oliveira Hélder Z. , Panza Patrícia S. , Ibáñez Sergio J. , Vianna Jeferson M.
Published: March 2021

This study examined individual, task, and environmental constraints that influence the career progression of youth Brazilian elite basketball players and the probability of reaching Novo Basquete Brasil (NBB) and to determine if the association of the relative age effect (RAE) is a key factor in the career progression. The sample consisted of 4,692 male players who were registered to participate in at least one U15, U17, or U22 youth Brazilian basketball championship between 2004 and 2018. Athletes who reached a high-performance level were coded like NBB players (9.6%). The birthdates, height, body mass, playing position, geographic region, club, competition category, and team performance were retrieved from the official data archive of the Brazilian Basketball Confederation and the National Basketball League. The maturity status was estimated using the predicted age at peak height velocity. A binary logistic regression examined the influence of each characteristic on the probability of a youth Brazilian basketball player to reach the NBB. The receiver operating characteristic (ROC) curves and the associated area under the curve (AUC) were used to assess the discriminant ability of the model. The taller and younger players not selected early into national teams, without specialization by playing position, who participated in U22 national championship, migrated to the southeast region, and remained in the formation process over time have a greater chance to reach the NBB. The ROC curve demonstrated an AUC of 93%. A combination of individual, task, and environmental characteristics influences the sport career of a young Brazilian basketball player in reaching the NBB. Further, early-maturing athletes have a greater chance to reach higher performances. RAE influences lower-level categories, but not a “NBB player's” career progression. The coaches, stakeholders, and practitioners should perform a holistic evaluation of sport talent in terms of a constraint-based theoretical model with the aim of avoiding bias produced by the maturational status and RAE in the youth Brazilian elite basketball.
Syllable Complexity and Morphological Synthesis: A Well-Motivated Positive Complexity Correlation Across Subdomains
Authors: Easterday Shelece , Stave Matthew , Allassonnière-Tang Marc , Seifart Frank
Published: March 2021

Relationships between phonological and morphological complexity have long been proposed in the linguistic literature, with empirical investigations often seeking complexity trade-offs. Positive complexity correlations tend not to be viewed in terms of motivations. We argue that positive complexity correlations can be diachronically well-motivated, emerging from crosslinguistically prevalent processes of language change. We examine the correlation between syllable complexity and morphological synthesis, hypothesizing that the process of grammaticalization motivates a positive relationship between the two features. To test this, we conduct a typological survey of 95 diverse languages and a corpus study of 21 languages with substantive (predominantly >10,000 words) corpora from the DoReCo project. The first study establishes a significant positive correlation between syllable complexity, measured in terms of maximal syllable patterns, and the index of synthesis (morpheme/word ratio). The second study tests the hypothesis that the relationship between syllable complexity and synthesis holds at local (word-initial and word-final) levels and within noun and verb types, as predicted by a grammaticalization account. While the findings of the corpus study are limited in their statistical power, the observed tendencies are consistent with our predictions. This study contributes important findings to the complexity literature, as well as a novel method which incorporates broad typological sampling and deep corpus analysis.
Autobiographical Memory and Social Identity in Autism: Preliminary Results of Social Positioning and Cognitive Intervention
Authors: Wantzen Prany , Boursette Amélie , Zante Elodie , Mioche Jeanne , Eustache Francis , Guénolé Fabian , Baleyte Jean-Marc , Guillery-Girard Bérengère
Published: March 2021

Autobiographical memory (AM) is closely linked to the self-concept, and fulfills directive, identity, social, and adaptive functions. Individuals with autism spectrum disorder (ASD) are now known to have atypical AM, which may be closely associated with social communication difficulties. This may result in qualitatively different autobiographical narratives, notably regarding social identity. In the present study, we sought to investigate this concept and develop a cognitive intervention targeting individuals with ASD. First, 13 adolescents with ASD and 13 typically developing adolescents underwent an AM interview featuring an original coding system designed to analyze the social self. We observed that the narratives produced by the ASD group focused more on the family than on extended social spheres, compared with those of the comparison group. Moreover, participants with ASD did not include themselves in the social groups they mentioned, and produced more references to others, compared with typically developing participants. Second, we designed a cognitive intervention program consisting of individual and group sessions that targeted AM. We conducted a pilot study among three adolescents with ASD aged 12, 16, and 17 years. Preliminary results showed that the program increased extra-family narrative references by the two youngest adolescents, who produced more social integration markers. Our study of autobiographical narratives yielded interesting findings about social positioning in ASD and showed how AM can be targeted in rehabilitation programs as a vector of social interaction.
Individual, Sociodemographic, and Environmental Factors Related to Physical Activity During the Spring 2020 COVID-19 Lockdown
Authors: Teran-Escobar Claudia , Forestier Cyril , Ginoux Clément , Isoard-Gautheur Sandrine , Sarrazin Philippe , Clavel Anna , Chalabaev Aïna
Published: March 2021

Background: Research has shown important between-individual variations in physical activity (PA) during the COVID-19 lockdown.

Objectives: The objectives of this is study are to examine the individual, sociodemographic, and environmental factors related to PA during the spring 2020 COVID-19 lockdown in France and to explore the mediating and moderating role of intention and self-efficacy toward PA in the relationships between sociodemographic/environmental variables and PA.

Design: In this cross-sectional study, participants living in France (N = 386) completed an online survey between March 30 and April 10, 2020.

Method: Minutes per week of moderate-to-vigorous PA during the lockdown; usual physical activity before the lockdown; and psychological (e.g., intention, self-efficacy, and autonomous and controlled motivation), sociodemographic (gender, age, and number of children), and environmental (habitat surface area and type of housing) factors were measured in the survey. Multiple linear regressions were used to investigate the role of these predictors on PA. Intention and self-efficacy were also examined as moderators and mediators of the association between sociodemographic/environmental factors and PA.

Results: Usual physical activity before the COVID-19 lockdown, intention toward PA, habitat surface area, and controlled motivation significantly predicted PA during the lockdown. No mediating effects of intention or self-efficacy were found. Intention significantly moderated the association between gender and PA and the association between part-time work and PA.

Conclusions: PA during the COVID-19 lockdown was mainly predicted by individual factors and notably usual PA. These results highlight the important role of habits in a highly changing context.
Albert Camus – A Psychobiographical Approach in Times of Covid-19
Authors: Mayer Claude-Hélène
Published: March 2021

Albert Camus (1913–1960) stands as one of the famous pioneers in the French history of existentialism. He was a novelist, political activist, essayist and editor, as well as a journalist and playwright. Although he was described as philosopher, he often denied this ascription. Through his professional and creative expressions, Camus focused on questions of existentialism, the aspect of the human fate, and meaning in life, death and suicide. These existential questions have experienced a strong revival during the Covid-19 occurrence. This psychobiographical approach aims at understanding Albert Camus' life and work in the context of the terror management theory of Becker and Wong's 4 pillars of PP2.0 theory, namely virtue, meaning, resilience and well-being. Both theories have gained importance during the pandemic. Based on the findings of the research study, implications for future research in the context of the pandemic are given. Finally, this article provides recommendations and best practices on how to approach the Covid-19 pandemic from a terror management theory and PP2.0 perspective in the light of Albert Camus' philosophy. The contribution of this psychobiography is two-fold: first, it expands psychobiographical research on Albert Camus from absurdist and existentialist theories and thereby expands the theoretical framework of psychobiographies. Second, it aims at strengthening the importance of theoretical psychobiographical investigations and their application in real-world scenarios to address complex contemporary challenges on the basis of existentialist positive psychology theories.
No Association Between Autonomic Functioning and Psychopathy and Aggression in Multi-Problem Young Adults
Authors: Zijlmans Josjan , Marhe Reshmi , van Duin Laura , Luijks Marie-Jolette A. , Bevaart Floor , Popma Arne
Published: March 2021

Background

Aberrant functioning of the autonomic nervous system (ANS) is an important factor in the occurrence of antisocial behavior. Baseline autonomic functioning and the responsivity of the ANS have been related to psychopathic traits and aggression. Here we investigated whether a naturalistic sample of male multi-problem young adults (age 18–27) present with similar autonomic deficits in relation to their psychopathy and aggression as previous studies observed in clinical samples.

Methods

In a sample of 112 multi-problem young adults, baseline autonomic functioning and autonomic responsivity to emotional stimuli were assessed through four physiological measures: heart rate, respiratory sinus arrhythmia, pre-ejection period, and skin conductance. 27 control participants were included primarily to assess whether the task worked appropriately. Participants watched a neutral 5 min video to assess baseline autonomic functioning and watched two sad clips to assess autonomic reactivity to sadness. We investigated the association between autonomic functioning and self-reported psychopathic traits and aggression within the multi-problem group.

Results

We found no significant associations between autonomic functioning and psychopathy and aggression.

Conclusion

These null-findings highlight the importance of research in naturalistic samples in addition to research in clinical and general populations samples and underscore the complexity of translating research findings into practical and clinical implications.
Naturalistic and Uncontrolled Pilot Study on the Efficacy of Vortioxetine in Binge Eating Disorder With Comorbid Depression
Authors: Segura-Garcia Cristina , Rania Marianna , Carbone Elvira Anna , de Filippis Renato , Aloi Matteo , Caroleo Mariarita , Grasso Gloria , Calabrò Giuseppina , Fazia Gilda , Staltari Filippo Antonio , Falvo Antonella , Pugliese Valentina , Gaetano Raffaele , Steardo Luca , De Fazio Pasquale
Published: March 2021

Background:Binge eating disorder (BED) is clinically relevant by virtue of the global impairment, poor quality of life, and increased overall medical morbidity. The high comorbidity with psychiatric disorders, particularly depression, has received attention as a possible mediator of the poor outcome. Further, BED and depression share cognitive dysfunctions. This naturalistic and uncontrolled pilot study aimed at evaluating the efficacy of vortioxetine (VTX) on depressive symptoms in patients with BED, secondly the efficacy in improving a broad array of executive functions, and third to explore the effect on eating behavior and body weight.

Methods:This pilot study involved 30 patients with BED and comorbid MDD, treated with VTX for 24 weeks. Assessments were run at baseline (t0), 4 (t1), 8 (t2), 12 (t3), and 24 (t4) weeks. Changes in depressive symptoms (HDRS and BDI), executive functions, eating behaviors (binge frequency and severity, night eating, food addiction), and body weight were estimated after treatment with VTX through GLM.

Results:Significant improvements emerged after treatment with VTX in: depression (HDRSp< 0.001; BDIp= 0.002) regardless the dose of VTX and first diagnosis (BED/MDD), working memory (RAVLT acquisitionp= 0.01, delay recallp< 0.001, RCFT percentage of recallp= 0.01, and Attentional Matricesp= 0.05), binge days frequency (p< 0.001), binge eating severity (BESp< 0.001), night eating (p= 0.001), food addiction (YFAS 2.0p= 0.039), and body weight (p= 0.039). The improvement in depressive symptoms was associated with the concurrent improvement in night eating as assessed by the I-NEQ.

Conclusions:VTX can be a valid therapeutic choice for patients with BED with comorbid depression in controlling the depressive symptoms, working memory, and eating behavior. Indeed, by acting on affective symptoms, neurocognitive functioning, and eating behaviors, it confirms the results already obtained with VTX in other disorders, expanding them to BED.
The Quixotic Task of Forecasting Peaks of COVID-19: Rather Focus on Forward and Backward Projections
Authors: Reis Ruy Freitas , Oliveira Rafael Sachetto , Quintela Bárbara de Melo , Campos Joventino de Oliveira , Gomes Johnny Moreira , Rocha Bernardo Martins , Lobosco Marcelo , dos Santos Rodrigo Weber
Published: March 2021

Over the last months, mathematical models have been extensively used to help control the COVID-19 pandemic worldwide. Although extremely useful in many tasks, most models have performed poorly in forecasting the pandemic peaks. We investigate this common pitfall by forecasting four countries' pandemic peak: Austria, Germany, Italy, and South Korea. Far from the peaks, our models can forecast the pandemic dynamics 20 days ahead. Nevertheless, when calibrating our models close to the day of the pandemic peak, all forecasts fail. Uncertainty quantification and sensitivity analysis revealed the main obstacle: the misestimation of the transmission rate. Inverse uncertainty quantification has shown that significant changes in transmission rate commonly precede a peak. These changes are a key factor in forecasting the pandemic peak. Long forecasts of the pandemic peak are therefore undermined by the lack of models that can forecast changes in the transmission rate, i.e., how a particular society behaves, changes of mitigation policies, or how society chooses to respond to them. In addition, our studies revealed that even short forecasts of the pandemic peak are challenging. Backward projections have shown us that the correct estimation of any temporal change in the transmission rate is only possible many days ahead. Our results suggest that the distance between a change in the transmission rate and its correct identification in the curve of active infected cases can be as long as 15 days. This is intrinsic to the phenomenon and how it affects epidemic data: a new case is usually only reported after an incubation period followed by a delay associated with the test. In summary, our results suggest the phenomenon itself challenges the task of forecasting the peak of the COVID-19 pandemic when only epidemic data is available. Nevertheless, we show that exciting results can be obtained when using the same models to project different scenarios of reduced transmission rates. Therefore, our results highlight that mathematical modeling can help control COVID-19 pandemic by backward projections that characterize the phenomena' essential features and forward projections when different scenarios and strategies can be tested and used for decision-making.
The New Frontier of Esports and Gaming: A Scoping Meta-Review of Health Impacts and Research Agenda
Authors: Kelly Sarah , Leung Janni
Published: March 2021

Given the rapid evolution of the gaming industry and the rising popularity of a hyper-connected, competitive esports version of online gaming, a meta-review of the impact of online competitive gaming upon health is timely. A scoping meta-review was conducted on 10 reviews that reported on any health consequences (physical, lifestyle, cognitive, mental, or social) of esports, online competitive gaming, or video gaming participation, as a player or spectator. While past reviews have examined health effects of video gaming, few have focused upon the newly evolved gaming context, incorporating both playing and streamed viewing, recognition as a professional sport, and potential career and exponential participation. Most past reviews have focused upon physical health impacts of video gaming among adolescents and young adults, but none have examined impacts of different forms of gaming participation in the new gaming era, and their potential differential health impacts. A scoping meta-review was undertaken on the physical, social, and psychological health outcomes of competitive online gaming and associated screen use, revealing a need for further review and research into lifestyle health outcomes including diet and sedentary behavior among young esports and competitive video gaming participants.
Case Report: Revisiting the Internal Mammary Artery Perforator Flap: Salvage Option for Circumferential Pharyngo-Esophageal Defects
Authors: Marsden Nicholas , Shukla Lipi , Grinsell Damien
Published: March 2021

Patients that present with pharyngeal strictures and pharyngocutaneous fistulas in the context of previous reconstruction and post-operative radiotherapy often report significant morbidity and reduction in quality of life. Reconstruction of such defects present a substantial clinical challenge requiring the importation of unirradiated vascularized tissue to facilitate healing in a friable, fibrotic, and vessel depleted tissue bed. The authors present a case report demonstrating an adaptation of the internal mammary artery perforator (IMAP) flap for reliable reconstruction of circumferential pharyngeal defects with primary tension free closure of the donor site. This technique avoids the use of free tissue transfer in a hostile, irradiated neck. The tubed IMAP flap is an excellent option, serving the purposes of reconstruction as well as addressing the patient's presenting issues of a chronic sinus and pharyngeal stricture inhibiting oral intake.
Voice Assessment in Patients With Obstructive Sleep Apnea Syndrome After Transoral Robotic Surgery
Authors: Huang Hsin-Hsin , Tsao Chien-Han , Wei James Cheng-Chung
Published: March 2021

Objectives: Removal of part of the tongue base, in combination with uvulopharyngopalatoplasty via transoral robotic surgery (TORS), for treating obstructive sleep apnea syndrome (OSAS) results in enlargement of the oropharynx and hypopharynx and change in the size of the resonance chamber. These procedures may also alter the laryngeal-hyoid bone complex, which is linked to vocal fold tension. Thus, there is the potential for change in phonation and pitch after surgery.

Study Design: Prospective, nonrandomized, institutional board-approved study.

Methods: From January to August 2018, 15 patients with OSAS receiving TORS underwent voice and pitch sampling. The multi-dimensional voice program (MDVP) was applied to the evaluation of preoperative sound parameters. Highest pitch and lowest pitch were obtained with real-time pitch software, with pitch synchronized to electronic organ or tuner. Subjects also completed the Voice Handicap Index-10 scale (VHI-10), to assess their subjective perception and to detect factors affecting the VHI-10 score. The relevant parameters were analyzed again 3 months after the operation.

Results: There was an increase in VHI-10 score 3 months after operation that did not reach statistical significance. There were also no significant differences in sound parameters. Increases in highest pitch (353.18 Hz shift to 387.99 Hz), highest semitone (F5# shift to F5#), lowest pitch (117.45 Hz shift to 131.42 Hz), and lowest semitone (C3 shift to C3) did not reach statistical significance. The increase in the lowest semitone was significantly related to change in VHI-10 score (r = −0.808, P = 0.028).

Conclusion: Patients with OSA undergoing TORS showed a negative correlation coefficient over 0.8 with change in VHI-10 score. That is, increase in the lowest semitone after operation correlated with increase in VHI-10 score which may cause perceive changes in subjective pronunciation.
Rescue and Conservation of Male Adult Alpacas (Vicugna pacos) Based on Spermatogonial Stem Cell Biotechnology Using Atomized Black Maca as a Supplement of Cryopreservation Medium
Authors: Valdivia Martha , Bravo Zezé , Reyes Jhakelin , Gonzales Gustavo F.
Published: March 2021

This is the first time that testicular tissue (n = 44) and isolated testicular cells (n = 51) were cryopreserved from alpaca testes 24 h postmortem. For this purpose, internally designed freezing media and cryopreservation protocols were used. Testicular tissue fragments (25 mg) and isolated testicular cells were frozen in MTDB (trehalose and black maca), MTD (trehalose), MSDB (sucrose and black maca), and MSD (sucrose) media. Isolated spermatogonial cells were cryopreserved in two ways, before and after proliferation in vitro. After cryopreservation, the percentage of cell viability in Group 1 (>50% of cell viability) by trypan blue did not show differences within each group (p > 0.05) but showed significant differences when comparing fragments with isolated cells (p < 0.05). Spermatogonial stem cells (SSC) were identified by flow cytometry as strong Dolichos biflorus agglutinin (sDBA) and mitochondrial activity of SSC as strongly positive for MitoSense (sMitoSense+) in intact mitochondria cells, weakly positive for MitoSense (wMitoSense+) in early apoptosis, and necrosis with 7-Aminoactinomycin-D positive (7-AAD). After freezing, in Group 1M (≥30% sMitoSense+), the fragments did not show differences between the media (p > 0.05), but in the isolated cells frozen in MSDB medium, 63.68 ± 8.90% (p < 0.05). In Group 2M (<30% sMitoSense+), necrosis (7AAD+) in MSDB medium was 27.03 ± 5.80%, and necrosis in isolated cells was 14.05 ± 9.3% with significant differences between these groups (p < 0.05); in sMitoSense+, the isolated cells (34.40 ± 23%) had a higher percentage than the fragments (12.4 ± 5.2) (p < 0.05). On the other hand, MSDB and MSD media were significantly higher for isolated cells than for fragments in sDBA+ (p < 0.05). On the other hand, the SSC (sDBA+) had significant differences (p < 0.05) between fresh cells 7.43 ± 1.3% (sDBA+) compared with those cryopreserved in MSDB medium 1.46 ± 0.34% (sDBA+). Additionally, the proliferated and cryopreserved SSC 6.29 ± 1.17% (sDBA+) did not show significant differences concerning the fresh cells (p > 0.05). In conclusion, the black maca showed antioxidant properties when it was included in the freezing medium and, therefore, improved the SSC's conservation of the alpaca. Furthermore, the proliferation of isolated cells in vitro produces a higher amount of SSC after thawing them for further preclinical or clinical work.
Plasmodium matutinum Transmitted by Culex pipiens as a Cause of Avian Malaria in Captive African Penguins (Spheniscus demersus) in Italy
Authors: Iurescia Manuela , Romiti Federico , Cocumelli Cristiano , Diaconu Elena Lavinia , Stravino Fiorentino , Onorati Roberta , Alba Patricia , Friedrich Klaus Gunther , Maggi Flavio , Magliano Adele , Ermenegildi Arianna , Carfora Virginia , Caprioli Andrea , De Liberato Claudio , Battisti Antonio
Published: March 2021

Avian malaria is a parasitic disease of birds caused by protozoa belonging to the genus Plasmodium, within the order Haemosporida. Penguins are considered particularly susceptible, and outbreaks in captive populations can lead to high mortality. We used a multidisciplinary approach to investigate the death due to avian malaria, occurred between 2015 and 2019, in eight African penguins (Spheniscus demersus) kept in two Italian zoos located in central Italy, and situated about 30 km apart. We also provided information about the presence and circulation of Plasmodium spp. in mosquitoes in central Italy by sampling mosquitoes in both zoos where penguin mortalities occurred. In the eight dead penguins, gross and histopathological lesions were consistent with those previously observed by other authors in avian malaria outbreaks. Organs from dead penguins and mosquitoes collected in both zoos were tested for avian malaria parasites by using a PCR assay targeting the partial mitochondrial conserved region of the cytochrome b gene. Identification at species level was performed by sequencing analysis. Plasmodium matutinum was detected in both dead penguins and in mosquitoes (Culex pipiens), while Plasmodium vaughani in Culex pipiens only. Parasites were not found in any of the PCR tested Aedes albopictus samples. Based on our phylogenetic analysis, we detected three previously characterized lineages: Plasmodium matutinum LINN1 and AFTRU5, P. vaughani SYAT05. In Culex pipiens we also identified two novel lineages, CXPIP32 (inferred morphospecies Plasmodium matutinum) and CXPIP33 (inferred morphospecies P. vaughani). Significantly, LINN1 and AFTRU5 were found to be associated to penguin deaths, although only LINN1 was detected both in penguins (along the years of the study) and in Culex pipiens, while AFTRU5 was detected in a single penguin dead in 2017. In conclusion, in our study Plasmodium matutinum was found to cause avian malaria in captive penguins kept in Europe, with Culex pipiens being its most probable vector. Our results are in agreement with previous studies suggesting that Culex pipiens is one of the main vectors of Plasmodium spp. in Europe and the Northern Hemisphere. Zoos maintaining captive penguins in temperate areas where Culex pipiens is abundant should be well aware of the risks of avian malaria, and should put every effort to prevent outbreaks, in particular during the periods when the number of vectors is higher.
Metabolic Reprogramming and Inflammatory Response Induced by D-Lactate in Bovine Fibroblast-Like Synoviocytes Depends on HIF-1 Activity
Authors: Quiroga John , Alarcón Pablo , Manosalva Carolina , Teuber Stefanie , Taubert Anja , Hermosilla Carlos , Hidalgo María Angélica , Carretta María Daniella , Burgos Rafael Agustín
Published: March 2021

Acute ruminal acidosis (ARA) occurs after an excessive intake of rapidly fermentable carbohydrates and is characterized by the overproduction of D-lactate in the rumen that reaches the bloodstream. Lameness presentation, one of the primary consequences of ARA in cattle, is associated with the occurrence of laminitis and aseptic polysynovitis. Fibroblast-like synoviocytes (FLS) are predominant cells of synovia and play a key role in the pathophysiology of joint diseases, thus increasing the chances of the release of pro-inflammatory cytokines. Increased D-lactate levels and disturbances in the metabolism of carbohydrates, pyruvates, and amino acids are observed in the synovial fluid of heifers with ARA-related polysynovitis prior to neutrophil infiltration, suggesting an early involvement of metabolic disturbances in joint inflammation. We hypothesized that D-lactate induces metabolic reprogramming, along with an inflammatory response, in bovine exposed FLS. Gas chromatography-mass spectrometry (GC-MS)-based metabolomics revealed that D-lactate disrupts the metabolism of bovine FLS, mainly enhancing glycolysis and gluconeogenesis, pyruvate metabolism, and galactose metabolism. The reverse-transcription quantitative PCR (RT-qPCR) analysis revealed an increased expression of metabolic-related genes, including hypoxia-inducible factor 1 (HIF-1)α, glucose transporter 1 (Glut-1), L-lactate dehydrogenase subunit A (L-LDHA), and pyruvate dehydrogenase kinase 1 (PDK-1). Along with metabolic disturbances, D-lactate also induced an overexpression and the secretion of IL-6. Furthermore, the inhibition of HIF-1, PI3K/Akt, and NF-κB reduced the expression of IL-6 and metabolic-related genes. The results of this study reveal a potential role for D-lactate in bFLS metabolic reprogramming and support a close relationship between inflammation and metabolism in cattle.
Phenotypical Characterization and Clinical Outcome of Canine Burkitt-Like Lymphoma
Authors: Aresu Luca , Agnoli Chiara , Nicoletti Arturo , Fanelli Antonella , Martini Valeria , Bertoni Francesco , Marconato Laura
Published: March 2021

In dogs, Burkitt-like lymphoma (B-LL) is rare tumor and it is classified as a high-grade B-cell malignancy. The diagnosis is challenging because of the similar histologic appearance with other histotypes, no defined phenotypical criteria and poorly described clinical aspects. The aim of the study was to provide a detailed description of clinical and morphological features, as well as immunophenotypical profile of B-LL in comparison with the human counterpart. Thirteen dogs with histologically proven B-LL, for which a complete staging and follow-up were available, were retrospectively selected. Immunohistochemical expression of CD20, PAX5, CD3, CD10, BCL2, BCL6, MYC, and caspase-3 was evaluated. Histologically, all B-LLs showed a diffuse architecture with medium to large-sized cells, high mitotic rate and diffuse starry sky appearance. B-phenotype of neoplastic cells was confirmed both by flow-cytometry and immunohistochemistry. Conversely, B-LLs were negative for BCL2 and MYC, whereas some cases co-expressed BCL6 and CD10, suggesting a germinal center B-cell origin. Disease stage was advanced in the majority of cases. All dogs received CHOP-based chemotherapy with or without immunotherapy. Despite treatment, prognosis was poor, with a median time to progression and survival of 130 and 228 days, respectively. Nevertheless, ~30% of dogs survived more than 1 year. An increased apoptotic index, a high turnover index and caspase-3 index correlated with shorter survival. In conclusion, canine B-LL shows phenotypical differences with the human counterpart along with features that might help to differentiate this entity from diffuse large B-cell lymphoma.
Detection of Oil Palm Disease in Plantations in Krabi Province, Thailand with High Spatial Resolution Satellite Imagery
Authors: Malinee Rachane , Stratoulias Dimitris , Nuthammachot Narissara
Published: March 2021

Oil palm (Elaeis guineensis) trees are an important contributor of recent economic development in Southeast Asia. The high product yield, and the consequent high profitability, has led to a widespread expansion of plantations in the greater region. However, oil palms are susceptible to diseases that can have a detrimental effect. In this study we use hyper- and multi-spectral remote sensing to detect diseased oil palm trees in Krabi province, Thailand. Proximate spectroscopic measurements were used to identify and discern differences in leaf spectral radiance; the results indicate a relatively higher radiance in visible and near-infrared for the healthy leaves in comparison to the diseased. From a total of 113 samples for which the geolocation and the hyperspectral radiance were recorded, 59 and 54 samples were healthy and diseased oil palm trees, respectively. Moreover, a WorldView-2 satellite image was used to investigate the usability of traditional vegetation indices and subsequently detecting diseased oil palm trees. The results show that the overall maximum likelihood classification accuracy is 85.98%, the Kappa coefficient 0.71 and the producer’s accuracy for healthy and diseased oil palm trees 83.33 and 78.95, respectively. We conclude that high spatial and spectral resolutions can play a vital role in monitoring diseases in oil palm plantations.
Sen2Grass: A Cloud-Based Solution to Generate Field-Specific Grassland Information Derived from Sentinel-2 Imagery
Authors: Hardy Tom , Kooistra Lammert , Domingues Franceschini Marston , Richter Sebastiaan , Vonk Erwin , van den Eertwegh Gé , van Deijl Dion
Published: March 2021

Grasslands are important for their ecological values and for agricultural activities such as livestock production worldwide. Efficient grassland management is vital to these values and activities, and remote sensing technologies are increasingly being used to characterize the spatiotemporal variation of grasslands to support those management practices. For this study, Sentinel-2 satellite imagery was used as an input to develop an open-source and automated monitoring system (Sen2Grass) to gain field-specific grassland information on the national and regional level for any given time range as of January 2016. This system was implemented in a cloud-computing platform (StellaSpark Nexus) designed to process large geospatial data streams from a variety of sources and was tested for a number of parcels from the Haus Riswick experimental farm in Germany. Despite outliers due to fluctuating weather conditions, vegetation index time series suggested four distinct growing cycles per growing season. Established relationships between vegetation indices and grassland yield showed poor to moderate positive trends, implying that vegetation indices could be a potential predictor for grassland biomass and chlorophyll content. However, the inclusion of larger and additional datasets such as Sentinel-1 imagery could be beneficial to developing more robust prediction models and for automatic detection of mowing events for grasslands.
Numerical Study of the Drying of Cassava Roots Chips Using an Indirect Solar Dryer in Natural Convection
Authors: Simo-Tagne Merlin , Tagne Tagne Ablain , Ndukwu Macmanus Chinenye , Bennamoun Lyes , Obounou Akong Marcel Brice , El Marouani Maryam , Rogaume Yann
Published: March 2021

In this work, an indirect solar dryer for drying cassava root chips was modelled and experimentally validated using the environmental conditions of Yaoundé in Cameroon and Yamoussoukro in Ivory Coast. The dryers were operational in natural convection mode. Resolution of the equations was achieved by finite differences and the 4th order of Runge–Kutta methods. A model was proposed for performing heat and mass transfer using thermophysical properties of cassava roots, and the obtained results were satisfactory for all conditions, with moisture content difference of less than 0.2 kg/kg between the experimental and theoretical results. The model showed that the core of the product takes more time to dry, which always prolongs the drying duration. The heat and mass transfer coefficients vary during the entire process of solar drying. The drying kinetics vary during the drying with values lower than 1.2 × 10−4 kg/(kg.s). The great gradients of humidity were observed in the thickness of the sample with a regular distribution of the temperature each drying time in the thickness of the sample.
Adsorptive and Surface Characterization of Mediterranean Agrifood Processing Wastes: Prospection for Pesticide Removal
Authors: Fernández-López José A. , Doval Miñarro Marta , Angosto José M. , Fernández-Lledó Javier , Obón José M.
Published: March 2021

The sustainable management of biomass is a key global challenge that demands compliance with fundamental requirements of social and environmental responsibility and economic effectiveness. Strategies for the valorization of waste biomass from agrifood industries must be in line with sustainable technological management and eco-industrial approaches. The efficient bioremoval of the pesticides imazalil and thiabendazole from aqueous effluents using waste biomass from typically Mediterranean agrifood industries (citrus waste, artichoke agrowaste and olive mill residue) revealed that these residues may be transformed into cost-effective biosorbents. Agrifood wastes present irregular surfaces, many different sized pores and active functional groups on their surface, and they are abundant in nature. The surface and adsorptive properties of olive mill residue, artichoke agrowaste and citrus waste were characterized with respect to elemental composition, microstructure, crystallinity, pore size, presence of active functional groups, thermal stability, and point of zero charge. Olive mill residue showed the highest values of surface area (Brunauer–Emmett–Teller method), porosity, crystallinity index, and pH of zero point of charge. Olive mill residue showed the highest efficiency with sorption capacities of 9 mg·g−1 for imazalil and 8.6 mg·g−1 for thiabendazole.
Influence of Calving Ease on In-Line Milk Lactose and Other Milk Components
Authors: Antanaitis Ramūnas , Juozaitienė Vida , Malašauskienė Dovilė , Televičius Mindaugas , Urbutis Mingaudas , Baumgartner Walter
Published: March 2021

The aim of our study was to determine how the ease of calving of cows may influence changes in lactose concentration and other milk components and whether these two factors correlate with each other. To achieve this, we compared data of calving ease scores and average percentage of in-line registered milk lactose and other milk components. A total of 4723 dairy cows from nine dairy farms were studied. The cows were from the second to the fourth lactation. All cows were classified according to the calving ease: group 1 (score 1)—no problems; group 2 (score 2)—slight problems; group 3 (score 3)—needed assistance; group 4 (score 4)—considerable force or extreme difficulty. Based on the data from the milking robots, during complete lactation we recorded milk indicators: milk yield MY (kg/day), milk fat (MF), milk protein (MP), lactose (ML), milk fat/lactose ratio (MF/ML), milk protein/lactose ratio (MP/ML), milk urea (MU), and milk electrical conductivity (EC) of all quarters of the udder. According to the results, we found that cows that had no calving difficulties, also had higher milk lactose concentration. ML > 4.7% was found in 58.8% of cows without calving problems. Cows with more severe calving problems had higher risk of mastitis (SCC and EC). Our data indicates that more productive cows have more calving problems compared to less productive ones.
Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs
Authors: Núñez Yolanda , Radović Čedomir , Savić Radomir , García-Casco Juan M. , Čandek-Potokar Marjeta , Benítez Rita , Radojković Dragan , Lukić Miloš , Gogić Marija , Muñoz María , Fontanesi Luca , Óvilo Cristina
Published: March 2021

This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA-seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT-NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.
Payments for Conservation of Animal Genetic Resources in Agriculture: One Size Fits All?
Authors: Juvančič Luka , Slabe-Erker Renata , Ogorevc Marko , Drucker Adam G. , Erjavec Emil , Bojkovski Danijela
Published: March 2021

Local livestock breeds in Slovenia have been eligible for financial incentives in the form of a fixed payment per livestock unit (LU) since 2002. The scheme has however not been successful in reversing the erosion of animal genetic resources (AnGR). This paper investigates an alternative, whereby incentive payments would better reflect breeders’ actual opportunity costs. The paper contributes to the limited existing body of knowledge related to the use of tender mechanisms in the design of the payments for agrobiodiversity conservation schemes (PACS), particularly for AnGR. Empirical findings draw on the results of a stated preference survey involving 301 farmers in Slovenia, engaging, or being potentially able to engage, in the rearing of local pig, sheep and goat breeds. Interval and logistic regression model results suggest that willingness to accept (WTA) conservation support significantly differs from actual payment levels. The estimated WTA was found to be 27% lower for the local sheep and goat breeds and 5% higher for the local pig breed, suggesting that differentiated incentive payments would provide a more cost-effective alternative. Additional analysis of breeders’ preferences and motives for engaging in local livestock breed production further informs understanding regarding AnGR conservation policy and the importance of accompanying actions to reverse negative population trends. These include reducing administrative barriers and enhancing the market valorisation of local breeds.
Characterization of 475 Novel, Putative Small RNAs (sRNAs) in Carbon-Starved Salmonella enterica Serovar Typhimurium
Authors: Houserova Dominika , Dahmer Donovan J. , Amin Shivam V. , King Valeria M. , Barnhill Emmaline C. , Zambrano Mike E. , Dean Meghan A. , Crucello Aline , Aria Kevin M. , Spector Michael P. , Borchert Glen M.
Published: March 2021

An increasingly apparent role of noncoding RNA (ncRNAs) is to coordinate gene expression during environmental stress. A mounting body of evidence implicates small RNAs (sRNAs) as key drivers of Salmonella stress survival. Generally thought to be 50–500 nucleotides in length and to occur in intergenic regions, sRNAs typically regulate protein expression through base pairing with mRNA targets. In this work, through employing a refined definition of sRNAs allowing for shorter sequences and sRNA loci to overlap with annotated protein-coding gene loci, we have identified 475 previously unannotated sRNAs that are significantly differentially expressed during carbon starvation (C-starvation). Northern blotting and quantitative RT-PCRs confirm the expressions and identities of several of these novel sRNAs, and our computational analyses find the majority to be highly conserved and structurally related to known sRNAs. Importantly, we show that deletion of one of the sRNAs dynamically expressed during C-starvation, sRNA4130247, significantly impairs the Salmonella C-starvation response (CSR), confirming its involvement in the Salmonella CSR. In conclusion, the work presented here provides the first-ever characterization of intragenic sRNAs in Salmonella, experimentally confirms that sRNAs dynamically expressed during the CSR are directly involved in stress survival, and more than doubles the Salmonella enterica sRNAs described to date.
Metabolic Profiling Analysis Reveals the Potential Contribution of Barley Sprouts against Oxidative Stress and Related Liver Cell Damage in Habitual Alcohol Drinkers
Authors: Park Hyerin , Lee Eunok , Kim Yunsoo , Jung Hye Yoon , Kim Kwang-Min , Kwon Oran
Published: March 2021

Chronic excessive alcohol consumption is associated with multiple liver defects, such as steatosis and cirrhosis, mainly attributable to excessive reactive oxygen species (ROS) production. Barley sprouts (Hordeum vulgare L.) contain high levels of polyphenols that may serve as potential antioxidants. This study aimed to investigate whether barley sprouts extract powder (BSE) relieves alcohol-induced oxidative stress and related hepatic damages in habitual alcohol drinkers with fatty liver. In a 12-week randomized controlled trial with two arms (placebo or 480 mg/day BSE; n = 76), we measured clinical markers and metabolites at the baseline and endpoint to understand the complex molecular mechanisms. BSE supplementation reduced the magnitude of ROS generation and lipid peroxidation and improved the glutathione antioxidant system. Subsequent metabolomic analysis identified alterations in glutathione metabolism, amino acid metabolism, and fatty acid synthesis pathways, confirming the role of BSE in glutathione-related lipid metabolism. Finally, the unsupervised machine learning algorithm indicated that subjects with lower glutathione reductase at the baseline were responders for liver fat content, and those with higher fatigue and lipid oxidation were responders for γ-glutamyl transferase. These findings suggest that BSE administration may protect against hepatic injury by reducing oxidative stress and changing the metabolism in habitual alcohol drinkers with fatty liver.
Epigallocatechin-3-Gallate Plus Omega-3 Restores the Mitochondrial Complex I and F0F1-ATP Synthase Activities in PBMCs of Young Children with Down Syndrome: A Pilot Study of Safety and Efficacy
Authors: Scala Iris , Valenti Daniela , Scotto D’Aniello Valentina , Marino Maria , Riccio Maria Pia , Bravaccio Carmela , Vacca Rosa Anna , Strisciuglio Pietro
Published: March 2021

Down syndrome (DS) is a major genetic cause of intellectual disability. DS pathogenesis has not been fully elucidated, and no specific pharmacological therapy is available. DYRK1A overexpression, oxidative stress and mitochondrial dysfunction were described in trisomy 21. Epigallocatechin-3-gallate (EGCG) is a multimodal nutraceutical with antioxidant properties. EGCG inhibits DYRK1A overexpression and corrects DS mitochondrial dysfunction in vitro. The present study explores safety profiles in DS children aged 1–8 years treated with EGCG (10 mg/kg/die, suspended in omega-3, per os, in fasting conditions, for 6 months) and EGCG efficacy in restoring mitochondrial complex I and F0F1-ATP synthase (complex V) deficiency, assessed on PBMCs. The Griffiths Mental Developmental Scales—Extended Revised (GMDS-ER) was used for developmental profiling. Results show that decaffeinated EGCG (>90%) plus omega-3 is safe in DS children and effective in reverting the deficit of mitochondrial complex I and V activities. Decline of plasma folates was observed in 21% of EGCG-treated patients and should be carefully monitored. GMDS-ER scores did not show differences between the treated group compared to the DS control group. In conclusion, EGCG plus omega-3 can be safely administered under medical supervision in DS children aged 1–8 years to normalize mitochondria respiratory chain complex activities, while results on the improvement of developmental performance are still inconclusive.