bluuebunny/crossref_metadata_2025 huggingface dataset:
Features of coagulation hemostasis in patients with polytrauma
Authors: Vyshynska M.B.
Published: April 2023

Background. Post-traumatic coagulopathy develops in 40 % of trauma patients and is the most common cause of death in them. The objective was to study and analyze changes in indicators of coagulation hemostasis, to evaluate the frequency of minor bleedings in patients with polytrauma. Materials and methods. A prospective observational cohort study was conducted among 35 patients with a diagnosis of polytrauma who were treated at the Municipal Non-Profit Enterprise “Lviv First Territorial Medical Association” of the St. Luke’s Hospital, in the anesthesiology unit with intensive care beds, from January 1, 2021 to August 1, 2022. The inclusion in the treatment of tranexamic acid on the first day after polytrauma was evaluated by dividing the obtained data into time intervals: the prehospital stage and the first 3 hours after admission; 3–8 and 8–24 hours after admission for treatment. Coagulation hemostasis was assessed by the international normalized ratio, partial thromboplastin time (PTT), level of total fibrinogen and D-dimers. The occurrence of minor bleedings was determined visually. Research stages: first day (d1), third day (d3) after polytrauma. Results. We studied indicators of coagulation hemostasis: on the first day, there is a significant increase in prothrombin time, a decrease in the prothrombin index and the level of total fibrinogen, an increase in the international normalized ratio and PTT. On the third day after injury, all studied indicators, with the exception of PTT, did not reliably differ from similar indicators of the control group. The level of D-dimers at all stages of the study was higher than normal values. After assessing the frequency of minor bleedings in patients 1–5 days after the injury, it was found that such bleedings were not associated with coagulation disorders. Conclusions. When analyzing the coagulation hemostasis, we have revealed that on the third day after injury, all indicators, except PTT, became normal. It was found that the occurrence of minor bleedings was not associated with coagulation disorders; therefore, they might be associated with vascular and platelet changes or fibrinolytic disorders.
Cultivating Interdisciplinary Foreign Language Talents in Higher Education in Western China under the Background of the “B&R”
Authors: Tian Hai Q. , Wei Yi J.
Published: April 2023

There is an increasing demand for interdisciplinary foreign language talents who master multiple foreign languages and cultures, innovation ability, and management ability, especially under the “Belt and Road” background. However, cultivating interdisciplinary foreign language talents in western China is facing many dilemmas. In order to meet the interdisciplinary foreign language talents demand of the international economic development along the “Belt and Road”, the author puts forward some strategies based on symbiosis theory adopting a comparative method. This article aims to explore methods of cultivating interdisciplinary foreign language talents in higher education in western China and supply high-quality interdisciplinary foreign language talents for the “Belt and Road”.
Analysis of the Influence of Modern Western Philosophical Thoughts on Contemporary College Students
Authors: Kexin Li
Published: April 2023

Modern Western philosophical thoughts mainly include: Western scientism, Western pragmatism, Western humanism and so on. These modern Western philosophical thoughts have exerted a wide influence on contemporary college students, both positive and negative aspects. This paper analyzes the influence of modern Western philosophical thoughts on contemporary college students.
The Emerging Paradigm of Antimicrobial Resistance in Surgical Site Infections of the Nilgiris Region
Authors: Begum P. Rihana , Rajeshkumar R. , Manigandan V. , Balasubramaniam V. , Ponnusankar Sivasankaran , Dhama Kuldeep , Emran Talha Bin
Published: April 2023

The rise of multidrug-resistant bacterial species in hospitals becomes a global challenge for surgeons who treat healthcare-associated infections. This study aimed to identify the pathogens involved in surgical site infections (SSI) as well as the prevalence of antibiotic resistant bacteria in the Nilgiris region. A hospital-based retrospective study was conducted for three years, at Microbiology Laboratory, the Govt. Medical College Hospital, where the clinical samples were collected, cultured, and identified. Antibiotic susceptibility was assessed using Kirby Bauer’s disc diffusion method. Out of 513 pus samples (from SSI), 242 (47%) have shown positive microbial growth. These isolates were evaluated for antimicrobial resistance using 20 antibiotics belonging to different groups. Staphylococcus aureus was found to be more prominent (69%), followed by Enterococcus species (14.5%) and Streptococcus species (10.3%). Other species like Proteus species, Klebsiella species, Escherichia coli, and Pseudomonas aeruginosa account for less than 2%. These results clearly indicate that Staphylococcus aureus was the leading cause of surgical site infections. Among the antibiotics studied, Staphylococcus aureus was found to be more resistant to Penicillin G (84%) followed by Ampicillin (23%). The high rate of antibiotic resistance highlighted the need for an antibiotic policy that encourages more rational use of antibiotics.
Central Line Associated Blood Stream Infection: Microbiological Profile and its Antimicrobial Susceptibility Pattern at Tertiary Care Centre
Authors: Darji Sachin M. , Patel Neha
Published: April 2023

Central venous catheters are commonly inserted to monitor patients with critical illnesses. Even when used to treat very ill patients, they are susceptible to widespread headaches, including central line-associated bloodstream infections (CLABSI). Central line-associated bloodstream infection (CLABSI) is one of the most significant HAIs, associated with excess mortality of 12–25%. To discover CLABSI cases, microbiological profiles, and their antimicrobial susceptibility. The study was conducted in an intensive care unit over a period of 12 months. 150 blood samples and catheter tips were collected for the culture of suspected or secondary bacteremia. CLABSI is described as being consistent with the CDC’s proposal. Automated VITEK 2 technology identifies bacterial isolates and investigates their antimicrobial susceptibility. Out of 150 samples, 50 showed no growth, 45 showed colonizers, 40 showed CLABSI, and 15 showed secondary infection. Fifty-five had positive blood cultures, 15 of whom had another source of infection. In our study, the CLABSI rate was 7.8/1000 central venous days. Rigorous implementation of the system and maintenance of the central line bundle are mandatory to prevent colonization.
Research Paper on App-Based Solution to Identify & Solve Disease in Plants
Authors: Shah Mr. Prasham , Kherde Mr. Anshul , Solav Mrs. Rasika , Warghane Mrs. Megha
Published: April 2023

Abstract: Crop diseases are a major threat to food security, but their rapid identification remains difficult in many parts of the world due to the lack of the necessary infrastructure. The combination of increasing global smartphone penetration and recent advances in computer vision made possible by deep learning has paved the way for smartphone-assisted disease diagnosis. We collected an fixed dataset of images of plant leaves of healthy and infected plants. The user interface is developed as an Android mobile app, allowing farmers to capture a photo of the infected plant leaves. It then displays the disease category along with the confidence percentage. It is expected that this system would create a better opportunity for farmers to keep their crops healthy and eliminate the use of wrong fertilizers that could stress the plants.
JB Placement Assessment
Authors: Varsha Gaddam , Madugula Vinay , Azmath Ammar Raza , Anusha Mrs.
Published: April 2023

Abstract: The placement assessment platform is a web-based application designed to facilitate the recruitment process for companies and organizations. The platform provides a user-friendly interface for students to take assessment tests, and evaluate candidate performance. Candidates can also use the platform to create profiles, take mock exams, and attend assessments. the placement assessment platform is an effective solution for organizations of all sizes looking to streamline their recruitment process and identify the most qualified candidates for their open positions. The placement assessment platform is a comprehensive solution for employers to assess the skills and capabilities of job candidates in a standardized and objective manner.
Voice Based App for Police PCR Van
Authors: Sakhare Prof. S. A. , Pawar Mayuri , Rohankar Samruddha , Thavkar Vedant , Bawankar Yugal , Ganorkar Yash
Published: April 2023

Abstract: The police Public Call Response (PCR) van is a crucial component of law enforcement agencies, providing rapid response and assistance to emergency situations. However, traditional communication methods between PCR vans and dispatch centres, such as radio or phone calls, can be inefficient and prone to miscommunication. In recent years, voice-based applications have gained popularity due to their ease of use and accessibility. Therefore, this abstract proposes the development of a voice-based app for police PCR vans to enhance communication, coordination, and efficiency in emergency response situations. The voice-based app will be designed as a mobile application that can be installed on smartphones or tablets used by police officers in PCR vans. The app will leverage voice recognition technology to enable officers to communicate with the dispatch centre using voice commands, eliminating the need for typing or manual input. The app will also have a text-to-speech feature that can convert dispatch centre responses into audible messages for the officers to hear. The app will include features such as real-time location tracking of PCR vans, allowing dispatch centres to accurately locate and assign tasks to the nearest available van. It will also support voice-based status updates from officers, allowing them to quickly report their current situation or request backup. To ensure secure communication, the app will have robust authentication and encryption measures to protect sensitive information. Additionally, the app will be designed to work offline in areas with poor network connectivity, with data synchronisation capabilities once network connection is restored. The proposed voice-based app for police PCR vans has the potential to significantly improve communication and coordination in emergency response situations. By leveraging voice recognition technology, officers can communicate quickly and efficiently, allowing them to focus on their primary responsibilities of ensuring public safety
Supply Chain Monitoring and Authentication Using Blockchain
Authors: Deepthi Ms. M. , Sree Kota Sneha Sai , Ramani Budda Venkata , Devi Yaramati Jaswini Lakshmi , Sharmila Ravula
Published: April 2023

Abstract: The Frozen Food industry produces and supplies packaged food products that are to be delivered at a particular freezing-points, therefore it is important that they are supplied in such an environment where they do not get damaged. To achieve this, continuous tracking of the shipped product must be monitored in a freezing environment where temperature and also humidity are maintained called supply chain management. During transportation of the packaged food, there may be a chance of getting damaged food like fluctuations in the temperature maintenance might cause food to be rotten. And there is a chance of leakage of canned goods or bulge at the ends which indicates the unhealthy and unhygienic condition of the packaged food. Quick Response (QR) codes provide a robust technique to fight the practice of counterfeiting the food products. Counterfeit food products are detected using a QR code scanner, where a QR code of the packaged good is linked to a Blockchain. And this system may be used to store shipped product details and generated unique code of that product as blocks in the database. So, we aim at two important aspects, firstly on continuous monitoring of supply chain and then on fake product detection which is done using Blockchain.
Electrical Inspection and Autometed Robot for Electrical Transmission Line Inspection
Authors: Sakore Miss. Shital , Mule Miss. Shradhha , Dorle Mr. Akash , Dhenge Prof. Kirti
Published: April 2023

Abstract: The paper presents sharing several of experiences and practices on smart robotic application for overhead transmission line maintenance and inspection. The robotic puller passes over barriers below the power line, such as the road with traffic, power distribution lines, river, or vegetation making tasks achieved conveniently, safely, and rapidly without impact on nearby communities.The target of the research is to create a mobile robot prototype for inspection of overhead power lines. The inspection robot shall crawl along the ground wire and transpose autonomously across installed equipment on the ground wire, such as vibration dampers, suspension clamps, compression dead ends, etc. In addition, the inspection robot is able to take photos and videos during a transmission line inspection . Using the robot, transmission line inspection’s labor cost can be reduced, and the new method helps improve patrol and inspection efficiency, comparing to the conventional manpower method. Trough utilization of the new maintenance and inspection robots, utilities can minimiz transmission line operation & maintenance budget.
Artificial Intelligence in Robotics: (Review Paper)
Authors: Chopra Ronit
Published: April 2023

Abstract: This article offers an overview of the dynamic intersection of artificial intelligence, robotics, and their impact on economic and organizational dynamics. We delve into the burgeoning research streams that explore the multifaceted consequences of these cutting-edge technologies in the fields of economics and management. Drawing from the diverse approaches adopted by scholars in this field, we provide insights into the implications of artificial intelligence, robotics, and automation for organizational design and firm strategy. We call for increased attention and involvement by organizational and strategy researchers in these areas and outline promising avenues for future research endeavors
Experimental Study on the Effects of Corrosion in Reinforced Concrete Incorporating Steel Fiber and Ultra-Fine Slag
Authors: Shukla Divyansh , Jha Abhay Kumar , Kumar Barun
Published: April 2023

Abstract: In this work, a thorough review of the literature was conducted with a primary focus on accelerated corrosion-induced corrosion. The report that follows compares different reinforced concrete mixtures following corrosion. By adjusting the transparent cover and reinforcement diameter, a simple reinforced concrete specimen, a specimen of reinforced concrete mixed with steel fiber, and a specimen of reinforced concrete mixed with steel fibre and ultra-fine slag were all produced. Using the accelerated corrosion method, the rate of induced corrosion, the flexural strength before and after corrosion, and the depth of corrosion penetration were compared.
Butea monosperma (Lam.) Taub: Review on its chemistry, morphology, ethnomedical uses, phytochemistry and pharmacological activities
Authors: Jain Sourabh , Dubey P. K.
Published: April 2023

Plants have been employed for their therapeutic and aesthetic benefits since ancient times. Traditional herbal medicine is well-documented and practised in India. The goal of the current review is to provide current information on botany, morphology, ecological biodiversity, medicinal uses, phytochemistry, and pharmacological activity on various portions of Butea monosperma (Lam.) Taub because there haven't been many studies done on it (B. monosperma). Technical literature from electronic search engines like Springerlink, BioMed Central, PubMed, Scopus, ScienceDirect, Scielo, Medline, and Science domain was used to compile this review. Books, book chapters, dissertations, websites, and other scientific publications served as the source of the supplemental texts. B. monosperma, a member of the Fabaceae family, is found throughout the Indian Subcontinent and Southeast Asia, including in Bangladesh, Nepal, Sri Lanka, Myanmar, Thailand, Laos, Cambodia, Vietnam, Malaysia, and western Indonesia. It is also known as Flame of the Forest, Dhak, Palash, or Bastard teak. It is an herb that has long been used voluntarily in traditional Asian treatments. It has been used to treat a variety of illnesses, including diabetes, cancer, diarrhoea, dysentery, fever, and jaundice. Recent in vivo and in vitro research have shown that it has hepatoprotective, anti-cancer, anti-diabetic, anti-inflammatory, anti-asthmatic, anti-oxidant, anti-convulsant, and anti-microbial effects. Many phytochemicals, primarily flavonoids, lactones, diterpenoids, diterpene glycosides, and phytosterols, are present in the plant's aerial portion. Bark produces a red fluid called "Butea gum" or "Bengal kino" that is produced. This complementary medical approach is becoming more and more well-known worldwide. Isolating the active components, testing them biologically, understanding their molecular mechanisms, mounting an experimental defence, and getting B. monosperma's medicinal usage approved are all necessary. The information gathered will be required to identify up study protocol for modern medications and Ayurveda formulation extension in luxurious and remedial a number of illnesses. To further validate the claims on humans, clinical trials for the stated preclinical investigations should be started right away.

Keywords: Butea monosperma, Fabaceae, Phytochemistry, Pharmacological activity, Ethno medical uses.
Unraveling the Multi-Target Pharmacological Mechanism of Brassica rapa in Diabetes Treatment: Integration of Network Pharmacology and Molecular Docking Approaches
Authors: Suresh Kumar Gopal , Manivannan Rangasamy , Nivetha Balasubramaniyan , Kamalakannan Dhanabalan , Bhuvaneshwari Kannan , Ammu Ramamurthy , Aravinthan Arumugam , Anitha Kuppusamy , Dinesh Kumar Vijayakumar
Published: April 2023

Brassica rapa has been widely reported as an anti-diabetic plant and is widely used in traditional medicine for the treatment of various disorders. However, the molecular mechanism underlying the plant's anti-diabetic activity has not been elucidated. Therefore, the present study aimed to investigate the possible molecular mechanism of B.rapa for managing diabetes mellitus through network pharmacology and molecular docking studies. The active ingredients and associated target proteins were obtained from a literature review and the Swiss Target Prediction platform and validated using the PubChem database. The disease-associated genes were retrieved from the Genecard database. The B. rapa-DM target network was analyzed using the STRING database, and the results were integrated and visualized using Cytoscape software. The molecular mechanism and therapeutic effect of B.rapa for the treatment of DM were determined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses using the Enrichr Platform. Fifty-two active ingredients were screened from B. rapa, and 1528 putative target genes were identified from these ingredients. Four hundred and fifty-four overlapping targets matched with DM were considered potential therapeutic targets. First, ten key targets (ALB, AKT1, TNF, GAPDH, MAPK3, EGFR, VEGFA, CTNNB1, CASP3, and STAT3) were found by topological analysis. Then, the results of GO and KEGG suggested that the anti-diabetes effect of B. rapa was strongly associated with the AGE-RAGE signaling pathway in diabetic complications, Neuroactive ligand-receptor interaction, Lipid and atherosclerosis, PI3K-Akt signaling pathway, and Calcium signaling pathway. The AKT1 (Serine/Threonine Protein Kinase) enzyme is targeted by major bioactive constituents of B.rapa. Molecular Docking studies revealed that Liquiritin (docking score -6.1 Kcal/mol) showed the highest binding affinity with AKT1. These results suggest that Brassica rapa may play a role in regulating several pathways that are involved in the development of Diabetes Mellitus.

Keywords: Network pharmacology, molecular mechanisms, Brassica rapa, Diabetes Mellitus (DM)
Novel Applications of Hot Melt Extrusion Technology
Authors: Samanthula Kumara Swamy , Kemisetti Durgaprasad , Mandhadi Jithendar Reddy , Thalluri Chandrashekar , Dey Biplab Kumar
Published: April 2023

Hot melt extrusion (HME) technology was introduced to pharmaceuticals in the 1970s for manufacturing and product development. Since then, there were several developments in HME technology to utilize it effectively for the manufacturing of pharmaceuticals. Though the primary purpose of HME technology remains to be solubility enhancement through the preparation of amorphous solid dispersions, it also has various other applications like dry granulation, abuse-deterrent formulation, continuous manufacturing, film preparation, implants, sustained release formulations, etc. The purpose of this review article is to consolidate the information related to applications of HME technology in the pharmaceutical industry.

Keywords: Hot melt extrusion, poorly soluble drugs, continuous manufacturing, solid orals, and applications.
Formulation Development and Evaluation of Sustained Release Ranolazine Microbeads using Natural Polymer
Authors: Dhone Shubham Dattarao , Kumawat Nikita Suresh , Kharat Dhanshree Raju , Parashar Komal Satish , Jagatap Vilas Raghunat , Sonawane Raju Onkar
Published: April 2023

The goal of this study was to develop sustained-release microbeads containing Ranolazine. The Ranolazine was selected on the basis of its short half-life i.e., 1.5 h and used in treatment of Angina pectoris. The fundamental goal of this research is to increase duration of drug release. Ranolazine was encapsulated with natural polymers such as Hygrophila auriculata seed mucilage by an ionotropic gelation technique. The formulation batches were optimized with a 32 factorial design and physicochemical characteristics were also evaluated. The particle size of microbeads, entrapment efficiency of drug, surface morphology, In-vitro release of drug was investigated.

The in-vitro studies of optimized microbeads formulation batch (B2) containing 100:600 ratio of Hygrophila auriculata seed mucilage and sodium alginate exhibited as a sustained release of Ranolazine up to 12 h and. The mucoadhesion potential was found to be 97± 1% up 12 h. In our perspective, the current ER pellet formulation might be the most feasible alternative to traditional pain management formulas.

All the trial’s batches were shown to be suitable in extended release of a short elimination half-life medication with enhanced bioavailability, implying that it is useful for oral drug delivery.

Keywords:  Design of experiment, Extrusion-spheronization, Ionic gelation, Microbeads, Ranolazine,
Seroprevalence and Risk Factors of Toxoplasmosis in Cats and Women Undergoing Prenatal Consultation in Coyah, Guinea
Authors: KONATE Yacouba , GOUMOU Pé , SOROMOU Lanan Wassy , DIALLO Alpha Oumar Sily , KABA Lancei , KEYRA Mohamed , CAMARA Mamadou Fodé. , SIDIME Youssouf
Published: April 2023

The objective of this study is to describe the epidemiological situation of toxoplasmosis in cats and women undergoing prenatal consultation in the city of Coyah (Guinea). During the study, 100 cat sera and 100 sera from women in prenatal consultation were analyzed with a Toxo-Screen DA (Modified Antigen Agglutination Test) kit. The results indicated prevalences of 73%±8.7 in antenatal women and 51%±9.8 in cats. There was no association found between the variables and Toxoplasma gondii seroprevalence in prenatal females and cats.

Keywords: Cat; women in prenatal consultation; Toxoplasma gondii; Coyah; Guinea.
Express method for particle size analysis in solid, liquid, and amorphous materials using 3D calibration curve and 2D-DLS dynamic light scattering
Authors: Kolyabina Ekaterina , Maksimova Tatyana , Syroeshkin Anton
Published: April 2023

This study proposes a new method for particle size studying based on the use of topological descriptors for various media and types of matter. The sizes of supramolecular particles in water-lactose complexes were studied using the diffuse dynamic light scattering (DLS) method. Mist-Standart latex balls were used to generate a 3D calibration curve, followed by empirical particle size determination. In order to confirm the output, the resulting sizes were compared for a number of silver proteinate solutions, preliminary analyzed by laser light scattering (using ZetaSizer equipment by Malvern Instruments Ltd, UK). The new method uses a simplified procedure, provides a quick solution and may be used for both quality control of cloudy and opaque medicinal substances and excipients as well as in other fields of nanotechnology.

Keywords: nanoparticles, particle size, silver proteinate, latex nanoparticles, dynamic light scattering
As contribuições da sinalização turística para o desenvolvimento do centro histórico de Parnaíba (Piauí – Brasil)
Authors: do Nascimento Melo Ronaldo
Published: April 2023

A sinalização turística é um processo participativo em que o atrativo precisa ser caracterizado por uma fonte de informação que esteja de fácil acesso para o visitante e comunidade local. O sistema de sinais é necessário para servir como o próprio “cartão de visita” do lugar, para que o turista não passe despercebido das informações dos atrativos contidas nas placas do centro histórico da cidade, sendo assim o turista não ficando impossibilitado de compreender o atrativo. O grande questionamento a ser enfrentado na conjuntura atual é a necessidade de uma sinalização turística no centro histórico de Parnaíba, que possa garantir para os visitantes e comunidade local uma melhor qualidade de experiências turísticas. A partir da análise que será verificada os turistas devem ser o principal alvo, pois as normas de orientação turística não são percebidas no local citado. A sinalização tem uma grande importância para identificar e definir o exato percurso que leva a pessoa ao local desejado. De acordo com a resolução 599/82 do Código de Transito Brasileiro – CTB: (BRASIL, 1982, s.p). “Os sinais de Trânsito são usados para orientar, advertir, e disciplinar a circulação dos elementos do trânsito ao longo das vias”. Nesse contexto é preciso existir um conjunto de regras que possam realizar o funcionamento do sistema de sinalização, incluindo uma ordem cronológica de normas precisas para realizar o seu funcionamento.
Cartographic reconstruction of nature management based on retrospective topographic maps
Authors: Beshentsev A.N. , Lubsanov A.A.
Published: April 2023

Taking the most developed part of the Baikal basin as example a digital cartographic reconstruction of the regional nature management of the early XX century is compiled; it enables establishing the physical and geographical characteristics of the territory economic use in the past. As a result of nature management objects retrospective layers vectorization and subsequent transformation of their shp files into the ArcGIS environment spatial object classes, two cartographic sets of such classes were formed. They characterize two types of regional nature use, traditional pastoral and invasive agricultural. As a result of layer-by-layer geoinformation modeling of cartographic sets on a modern topographic basis and a digital relief model, primary areas of settlement and economic use of the territory were established, and various mapping of spatial and temporal life support structures of nature management different types was performed. For operational digital visualization, a technological solution is proposed for storing and mapping sets of classes of spatial objects through arrangement of a file-based geodata of the ArcGIS information environment. Cartographic reconstructions of pastoral and agricultural nature management at the beginning of the XX century are presented, as well as its general cartographic reconstruction on landscape basis.
Sequential design of a multi-level information base for land inventory
Authors: Pavlova V.A. , Stepanova E.A. , Uvarova E.L.
Published: April 2023

There is an extensive amount of research works in the field of land inventory. However, in modern scientific and technical literature, they do not deal with designing a multi-level information base for the inventory of agricultural land, a priority category of land. There is practically no detailed description of the database formation in existing papers. The aim of the work is consistent designing a bank of data for the agricultural land inventory. So, the authors discuss the procedure for carrying it out, provide an analysis of modern technologies in order to form semantic and graphical parts of the information base, and outline the features of its sequential design. The research methods of theoretical and empirical level were used. We have presented the automation for the process of entering data into the base using GIS technologies. As a result, the final outcome was obtained, a multi-level bank for land inventory. The approbation results of the proposed production are presented on the materials of the Pskov oblast.
Rules and regulations for enhancing metro rail accessibility in a developing country
Authors: Satranarakun Atipon , Kraiwanit Tanpat
Published: April 2023

This study aims to investigate the variables affecting the accessibility of rail transit services for Bangkok and its surrounding residents and the problems in establishing a central clearing house (CCH) to develop rules and regulations for a common ticketing system in Thailand. This study employed mixed methodologies — a combination of quantitative and qualitative methodologies. For data analysis, binary logistic regression analysis and content analysis were employed. Currently, a relatively small fraction of commuters takes metro trains (Satranarakun & Kraiwanit, 2021). Access to rail transit services among users and all non-users is influenced by the number of transfers, city of residence, monthly transportation costs, monthly expenses, and use of Pinterest, WhatsApp, YouTube, Facebook, private vehicles, and motorcycle taxis. Access among users and potential non users is influenced by using Pinterest, motorcycle taxis, and private vehicles. CCH should be administered by a government agency or an impartial organisation. Service providers should advertise and launch promotions via social networks and place emphasis on those with the potential to pay for transportation but not use the services. Metro rail systems should collaborate with community organisations and advocates to develop programs and initiatives that address the specific needs of vulnerable populations while also promoting universal access to public transportation. Overall, metro rail accessibility laws, rules, and regulations should prioritise affordability and accessibility for all individuals.
The impact of country branding on competitive advantage: Evidence from the commercial aviation industry
Authors: Salaheldin Karim Mamdouh Ahmed , Mubarak Abdel-Kader Mohamed Abdel-Kader Ahmed , Dakrory Mona Ibrahim
Published: April 2023

This study aims to investigate the direct effect of country branding on competitive advantage, applying it to the commercial aviation industry in Egypt. The results of the analysis showed that there is a positive relationship between the country branding and competitive advantage in Egyptian commercial aviation industry. The study reached a number of results, the most important of which are: the necessity of concluding strategic alliances for the purpose of establishing new airports and developing the existing ones, increasing the air fleet, in order to accommodate and increase the number of flights and achieve a competitive advantage, thus building a good reputation for the country and building a brand for the nation, which contributes to attracting more air traffic.
An EphA2 monobody as a probe for colorectal cancer
Authors: Venu Akhil , Zhang Ying , Seong Jihyoung , Hong Yeongjin , Lee Wan-Sik , Min Jung-Joon
Published: April 2023

Bakground Colorectal cancer (CRC) is the third most common cancer
worldwide and the second most common cause of cancer-related death.
Various molecular markers are used for early CRC diagnosis. Of these,
human ephrin receptor family type-A 2 (hEphA2) oncoprotein is
overexpressed significantly at the early, but not late, stages of CRC.
Previously, we reported an E1 monobody that is specific for
hEphA2-expressing cancer cells both in vitro and in vivo. Aim We aimed
to use E1 monobody as a detection probe for hEPhA2 expressing colorectal
cancer. In addition, we investigated the efficacy of E1 monobody to
target on human colorectal cancer tissue. Method Here, we confirmed the
expression of hEPhA2 on the surface of colorectal cancer cells by
western blotting and flow cytometry. We evaluated the targeting efficacy
of E1 monobody on colorectal cancer cells by flow cytometry and further
confirmed with immunofluorescence staining. E1 conjugated to the Rluc8
reporter protein were used as imaging agent for in vivo imaging.
Additionally, EGFP conjugated E1 monobody were used to check the
targeting ability of E1 monobody on human colorectal cancer tissue.
Result E1 bound efficiently to nine hEphA2-expressing CRC cell lines and
E1 conjugated to the Rluc8 reporter protein targeted tumor tissues in
mice transplanted with HCT115 CRC tumor cells. Finally, E1-EGFP stained
tumor tissues from human CRC patients, showing a pattern similar to that
of an anti-hEphA2 antibody. Conclusion These results suggest that the E1
monobody has utility as a probe to detect CRC.
A Versatile, Electrophilic Reagent for Monofluoromethylthiola-tion
Authors: Wang Rui , Lv Long , Shen Qilong
Published: April 2023

A new electrophilic monofluoromethylthiolating reagent
N-fluoromethylthiophthalimide PhthSCH2F 1 was developed. Reagent 1 could
be readily synthesized from easily available starting materials benzyl
mercaptan and CH2FCl in three steps. N-fluoromethylthiophthalimide 1 is
a powerful electrophilic monofluoromethylthiolating reagent that allows
the monofluorome-thylthiolation of a wide range of nucleophiles
including alkynes, aryl/vinyl boronic acids, electron-rich
heteroarenes,-ketoesters and oxindoles, as well as thiols under mild
conditions.
An insight to the microbiota in the trophosome of the cold seep tubeworm, Lamellibrachia sp. from the Cauvery-Mannar basin
Authors: Unnikrishnan Akshay , Thakur Narsinh L. , Mazumdar Aninda
Published: April 2023

Cold seeps are unique ecosystem deep in the ocean, driven by the
chemosynthetic based benthic communities. One of the unique organisms
found in this ecosystem is the polychaete annelid tubeworm belonging to
the Siboglinidae family. This organism depends on sulfur oxidising
endosymbionts that are housed in the bacteriocytes for its nutrition.
However, little information is available on the complete microbiota
present in the trophosome. The detailed investigation was carried out by
employing a 16S rDNA based metagenomic analysis of the trophosome from
the tubeworm collected from the Mannar basin pockmark area in the Indian
Ocean. Despite being dominated by sulfur oxidizing bacteria, there was a
high percentage of OTUs belonging to Pseudomonales and Bacteroidales.
These bacteria may be involved in the metabolism of methane and complex
polymers which aids in the nutrition of the tubeworm and requires
further investigation with regards to their role. The sulfur oxidizing
bacteria showed more than 99% identity with symbiont sequences of
Escarpia sp and other Lamellibrachia sp from the Western
Mediterranean Sea and Gulf of Mexico respectively. This data constitutes
the first report on the bacterial community present in the trophosome of
the cold seep tubeworm Lamellibrachia sp from the pockmark of
Mannar basin.
Changes in IgG4 and IL-10 expression in adults with eosinophilic esophagitis on a two-food elimination diet
Authors: Santonicola Antonella , Appanna Ramapraba , Gargano Domenico , Caputo Alessandro , Bartolomeis Fabio De , Ricciardi Luca , Stefanelli Berenice , Caiazza Laura , Guarciariello Marialuisa , D’Antonio Antonio , D’Auria Raffaella , Conti Valeria , Casolaro Vincenzo , Iovino Paola
Published: April 2023

Background: Eosinophilic esophagitis (EoE) is increasingly
diagnosed in patients with dysphagia and upper gastroenteric symptoms.
Elimination diets and/or pharmacologic agents may accomplish temporary
remission, but long-term control is challenging. Type-2 immunity to
ingested antigens can induce EoE histopathology via non-IgE-dependent
mechanisms, possibly involving IgG4 and IL-10 production. To elucidate
the contribution of IgG4- and IL-10-producing cells to EoE pathogenesis,
we examined their frequencies and association with clinical and
histologic endpoints in adult EoE patients given a two-food elimination
diet (TFED). Methods: Sixteen patients with EoE were prescribed
a TFED. Biopsies collected at baseline and follow-up were used for
immunofluorescent detection of IgG4- and IL-10-expressing cells and
serum food-specific IgG4 were measured. All variables were correlated
with established histologic measures of disease activity.
Results: Patients exhibited significant clinical improvement
and significant reduction in esophageal eosinophilia and overall
histology. A significant decrease in the frequencies of IL-10-expressing
cells was also observed, which correlated with histologic changes. In
contrast, a concomitant decline in serum and esophageal IgG4, while
substantial, did not correlate with IL-10 -cell
frequencies or any histologic parameter of EoE activity.
Conclusions: The close association of esophageal IL-10
expression with histologic features and their changes after a TFED
suggests a critical role of this cytokine in EoE pathogenesis.
Conversely, IgG4 serum and mucosal expression, while reflecting the
level of exposure to relevant food antigens, is not obviously related to
EoE histopathology or IL-10 expression. Studies are needed to
characterize IL-10 cellular sources and their functions in EoE
progression and treatment response.
Impacts of physiological characteristics and human activities on the species distribution models of orchids taking the Hengduan Mountains as a case
Authors: wang xueman , Peng Pei-Hao , Bai Mao-Yang , Bai Wen-Qian , Zhang Shi-Qi , Feng Yu , Wang Juan , Tang Ying
Published: April 2023

The biogeography research of orchids through species distribution models
(SDMs), a vital tool in the biogeography field, is critical to
understanding the fundamental geographic distribution patterns and
identifying conservation priorities. The correspondence between species
occurrence and environmental information is crucial to the model’s
performance. However, ecological preferences unique to different orchid
species, such as their life forms, are often overlooked during the
modeling process. This oversight can introduce bias and increase model
uncertainty. Additionally, human activities, as an important potential
predictor, have not been quantified in any orchid SDMs. Taking the
Hengduan Mountains as an example, we preprocessed all orchid species’
occurrences based on physiological characteristics. Choosing five
spatial factors related to human activities to quantify the interference
and enter into models as HI factor. Using different modeling methods
(GLM, MaxEnt, and RF) and evaluation indices (AUC, TSS, and Kappa),
diverse modeling strategies have been constructed in the study. A
double-ranking method has been adopted to select the critical orchid
distribution regions. The results showed that classification models
based on physiological characteristics significantly improved the
model’s accuracy while adding the HI factor had the same effect but the
absence of enough significance. Suitability maps indicated that highly
heterogeneous mountainous areas were vital for the distribution of
orchids in the Hengduan Mountains. Different distribution patterns and
critical regions existed between various orchid life forms
geographically - terrestrial orchids were dominant in the mountain, and
mycoherterophical orchids were primarily located in the north, more
influenced by vegetation and temperature. Critical regions of epiphytic
orchids were in the south due to a greater dependence on precipitation
and temperature. These studies are informative for understanding the
orchids’ geographic distribution patterns in the Hengduan Mountains,
promoting conservation, and providing references for similar research
beyond orchids.
Sensitivity Analysis of a layered piezoelectric system using ZFEM
Authors: Acosta Carlos , Bhalla Amar , Guo Ruyan
Published: April 2023

This work presents an investigation on the numerical simulation of a
layered piezoelectric system using the complex finite element method
(ZFEM). This approach allows the standard FEM solution to provide
information about the design sensitivity of the mechanical displacement
and voltage potential fields with respect to small variations in the
material properties of the system. The layered body is formed by PZT-4
and PZT-5 stacked together. Results show that the design sensitivities
of the soft grounded piezoelectric are larger compared to those of PZT-4
for the specific configuration analyzed. For solution verification
purposes. The standard steady state solution was compared against a
commercial FEM package and the error obtained was less than 4%.
Observation on the ultrastructure of the oral appendages of Glenea cantor Fabricius (Coleoptera: Lamiinae)
Authors: wu yao , Li Zhixin , Ming Liangshan , Huang Zhongyan , Zhang Gaochuan , LU WEN , Liu Hongning , DONG ZISHU
Published: April 2023

We investigated the gross and ultrastructure morphology of the
mandibles, labial palpus and mandibular palpus of adult male and female
Glenea cantor beetles using scanning electron microscopy. There
were significant differences between males and females in the gross and
ultrastructure morphology of these parts. Observation of the gross
morphology of the mandible, labial palpus and mandibular palpus revealed
that their lengths were significantly greater in females, which is
mainly related to the oviposition mechanism. In terms of the
ultrastructural morphology of the mandibles, labial palpus and
mandibular palpus, seven types of sensilla were found on these parts:
sensilla twig basiconica (STB I, II, III, and IV), sensilla chaetica
(SCh IV and V), Böhm’s bristles (Bb), sensilla placodea (SP), sensilla
trichodea (ST II), sensilla plate (SP), sensilla coeloconica (SC) and
sensilla campaniformia (SCa). Females have significantly more ST I and
SCh III on their mandibles than males. The mechanical sensors SCh and
Bb, olfactory sensor ST II, taste sensor STB IV, and carbon dioxide
sensor and temperature and humidity sensors SC, SP and SCa were
significantly more developed on female than male labial and maxillary
palps, which also further explained the important role of the oral
appendages in oviposition behavior. These results will help us further
understand the host selection and oviposition behavior of adult G.
cantor and promote the information-based design of pest control
methods.
Expression of a ZnT8/GAD65 chimera in insect cells useful for Autoimmune Diabetes diagnosis
Authors: Trabucchi Aldana , Bombicino Silvina , Sabljic Adriana Victoria , Marfia Juan , Targovnik Alexandra , Iacono Ruben , Miranda Maria , Valdez Silvina
Published: April 2023

The combined presence of autoantibodies to the 65 kD isoform of glutamic
acid decarboxylase (GADA) and to the islet-specific cation efflux
transporter ZnT8 (ZnT8A) in serum is the best predictive sign of the
loss of immune tolerance and the clinical manifestation of autoimmune
diabetes mellitus (DM). The screening of GADA and ZnT8A could be an
appropriate alternative to identify diabetic subjects with underlying
autoimmunity, helping to reach to a correct diagnosis and guaranteeing
the start of an early and adequate treatment. Herein, we describe the
expression of a chimera molecule including immunodominant regions of the
antigens ZnT8 and GAD65 by using the baculovirus-insect cells system,
yielding 30 mg/L culture medium. This recombinant chimera retains the
immunoreactive conformation of the epitopes that are recognized by their
specific antibodies, so it was used for the development of a high
sensitivity (75.51 %) and specificity (98.04 %) bridge ELISA for the
detection of highly prevalence ZnT8A and/or GADA, in a one-step
screening assay. This immunoassay is useful either to confirm autoimmune
diabetes or for detection in routine screening of individuals at risk of
autoimmune DM. As DM is a slow progress disease, remaining asymptomatic
for a long preclinical period, serological testing is of importance to
establish a preventive treatment.
THE CHANGES IN ACTIVITY OF ATPase AND ANTIOXIDANT ENZYMES, AND PROLINE BIOSYNTHESIS IN YEASTS Candida guilliermondii NP-4 UNDER INFLUENCE OF X-IRRADIATION
Authors: Marutyan Seda , Karapetyan Hasmik , Marutyan Syuzan , Muradyan Anna , Badalyan Hamlet
Published: April 2023

This study aimed to investigate the effect of X-radiation on the ATPase
activity and the activity of antioxidant enzymes, including enzymes of
proline biosynthesis, in C. guilliermondii NP-4 yeast cells after
X-irradiation and the post-irradiation repair of cells. It has been
shown that the X-irradiation of yeasts C. guilliermondii NP-4 at
a dose of 300 Gy causes the increase of activity of the enzymes catalase
and SOD and the ratio of catalase/SOD. The repair of radiation-induced
damages requires a lot of energy, so the demand for ATP increases in
irradiated and repaired yeasts. The total and FoF
-ATPase activity in yeast homogenates and mitochondria
increases after X-irradiation and post-irradiation repair. Amino acid
proline is an antioxidative defense molecule and plays a significant
role in the adaptation of cells to stress. So higher proline levels in
yeasts can protect them from oxidative damage. There are still no
systematic studies of the antioxidant properties of proline. In this
study, it has been shown that the activity of enzymes of proline
biosynthesis (ornithine transaminase and proline-5-carboxylate
reductase) in yeasts C.guilliermondii NP-4 increases after
X-irradiation and remains higher after post-irradiation repair.
Connected with it, the proline amount in X-irradiated and repaired
yeasts also is higher than in non-irradiated cells. We suppose that
proline has a radioprotective effect on X-irradiated yeasts C.
guilliermondii NP-4.
Evaluating Subjective Well-being in the Network Society
Authors: Koo Nahoi
Published: April 2023

This study evaluates whether the network society is a happy society by modeling the methodology of happiness economics, which quantitatively measures the relationship between subjective well-being and its predictors. Using structural equation modeling, the study investigated the effects of two main characteristics of the network society - social network and information flow - on subjective well-being. To construct a model of subjective well-being, 1940 respondents of the U.S. population were sampled from the 2010-2012 World Values Survey data. Responses to the selected thirteen questions in the survey were then incorporated into the structural equation system to predict one's subjective well-being. SPSS, PRELIS, and LISREL 9.2 were used to perform data analyses under the assumptions of maximum likelihood estimates. Findings from the study demonstrated that: (1) Inclusion in social network had a positive impact on one's level of subjective wellbeing; (2) One's access to information had a positive effect on subjective well-being.