bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-Compassion as a Key Factor of Subjective Happiness and Psychological Well-Being among Greek Adults during COVID-19 Lockdowns
Authors: Sotiropoulou Kyriaki , Patitsa Christina , Giannakouli Venetia , Galanakis Michail , Koundourou Christiana , Tsitsas Georgios
Published: July 2023

The present study examined the association and complementary effect of self-compassion on the subjective happiness and psychological well-being of adults during the COVID-19 pandemic. The study was based on a concurrent correlational design to examine relationships between self-compassion, subjective happiness, psychological well-being, resilience, and the meaning in life. Data were collected via a battery of questionnaires and analyzed, focusing on the above variables. The sample of this study (N = 526) consisted of Greek professionals in education and university students. The results showed that there is a strong positive relationship between self-compassion and subjective happiness, and between self-compassion and psychological well-being. The findings suggest that an attitude of self-compassion may well influence the development of psychological well-being and increase the subjective happiness of adults during the distressing era of a long-term pandemic. The results also indicated a positive relationship between self-compassion and meaning in life and showed that self-compassion is a prerequisite for resilience, which in turn may serve as a moderator of psychological well-being and subjective happiness.
Measuring Depression in Young Adults: Preliminary Development of an English Version of the Teate Depression Inventory
Authors: Ruan-Iu Linda , Pendergast Laura L. , Liao Pei-Chun , Jones Paul , von der Embse Nathaniel , Innamorati Marco , Balsamo Michela
Published: July 2023

Depression is a common and debilitating condition that impacts individuals with various cultural backgrounds, medical conditions, and life circumstances. Thus, assessment tools need to be useful among different cultural groups. The 21-item Teate Depression Inventory (TDI) was developed in Italy, is designed to assess major depression, and focuses on cognitive and affective rather than somatic symptoms. This study aims to examine the factor structure and concurrent validity of the TDI English version among a non-clinical population in the United States. Participants included 398 adults (mean age 19.89 years, SD = 2.72, range: 18 to 46 years old) who completed the TDI and The Center for Epidemiologic Studies Depression Scale-Revised (CESD-R). The results supported a three-factor bifactor structure of the TDI (Positive Affect, Negative Affect, and Daily Functioning), which largely corresponds to the Tripartite Model of affective disorders. These findings support the use of TDI scores as measures of depressive symptoms among U.S. young adults, offering researchers and practitioners a brief and useful tool.
Aspirin Affects MDA-MB-231 Vesicle Production and Their Capacity to Induce Fibroblasts towards a Pro-Invasive State
Authors: Louback Rafaela de Assiz , Martins-Cardoso Karina , Tinoco Luzineide W. , Collino Federica , de Barros Ana Paula D. N. , Fortuna-Costa Anneliese , Monteiro Robson Q. , Rossi Maria Isabel Doria , Lindoso Rafael Soares
Published: July 2023

Long-term administration of aspirin (ASA, acetylsalicylic acid) in oncogenic patients has been related to a reduction in cancer risk incidence, but its precise mechanism of action is unclear. The activation of cancer-associated fibroblasts (CAFs) is a key element in tumor progression and can be triggered by cancer-derived extracellular vesicles (EVs). Targeting the communication between cancer cells and the surrounding tumor microenvironment (TME) may control cancer progression. Our aim was to investigate the effect of ASA on breast cancer cells, focusing on EV secretion and their effect on the biological properties of CAFs. As a result, ASA was shown to reduce the amount and alter the size distribution of EVs produced by MDA-MB-231 tumor cells. Fibroblasts stimulated with EVs derived from MDA-MB-231 treated with ASA (EV-ASA) showed a lower expression of alpha-smooth muscle actin (α-SMA), matrix metalloproteinase-2 (MMP2) but not fibroblast activation protein (FAP) in respect to the ones stimulated with EVs from untreated breast cancer cells (EV-CTR). Furthermore, invasion assays using a three-dimensional (3D) fibroblast spheroid model showed reduced MDA-MB-231 invasion towards fibroblast spheroids pretreated with EV-ASA as compared to spheroids prepared with EV-CTR-stimulated fibroblasts. This suggests that ASA partially inhibits the ability of tumor EVs to stimulate CAFs to promote cancer invasion. In conclusion, ASA can interfere with tumor communication by reducing EV secretion by breast tumor cells as well as by interfering with their capacity to stimulate fibroblasts to become CAFs.
RNA-Seq Virus Fraction in Lake Baikal and Treated Wastewaters
Authors: Potapov Sergey , Gorshkova Anna , Krasnopeev Andrey , Podlesnaya Galina , Tikhonova Irina , Suslova Maria , Kwon Dmitry , Patrushev Maxim , Drucker Valentin , Belykh Olga
Published: July 2023

In this study, we analyzed the transcriptomes of RNA and DNA viruses from the oligotrophic water of Lake Baikal and the effluent from wastewater treatment plants (WWTPs) discharged into the lake from the towns of Severobaikalsk and Slyudyanka located on the lake shores. Given the uniqueness and importance of Lake Baikal, the issues of biodiversity conservation and the monitoring of potential virological hazards to hydrobionts and humans are important. Wastewater treatment plants discharge treated effluent directly into the lake. In this context, the identification and monitoring of allochthonous microorganisms entering the lake play an important role. Using high-throughput sequencing methods, we found that dsDNA-containing viruses of the class Caudoviricetes were the most abundant in all samples, while Leviviricetes (ssRNA(+) viruses) dominated the treated water samples. RNA viruses of the families Nodaviridae, Tombusviridae, Dicitroviridae, Picobirnaviridae, Botourmiaviridae, Marnaviridae, Solemoviridae, and Endornavirida were found in the pelagic zone of three lake basins. Complete or nearly complete genomes of RNA viruses belonging to such families as Dicistroviridae, Marnaviridae, Blumeviridae, Virgaviridae, Solspiviridae, Nodaviridae, and Fiersviridae and the unassigned genus Chimpavirus, as well as unclassified picorna-like viruses, were identified. In general, the data of sanitary/microbiological and genetic analyses showed that WWTPs inadequately purify the discharged water, but, at the same time, we did not observe viruses pathogenic to humans in the pelagic zone of the lake.
Evolution of Vaccines Formulation to Tackle the Challenge of Anti-Microbial Resistant Pathogens
Authors: Tognetti Francesco , Biagini Massimiliano , Denis Maxime , Berti Francesco , Maione Domenico , Stranges Daniela
Published: July 2023

The increasing diffusion of antimicrobial resistance (AMR) across more and more bacterial species emphasizes the urgency of identifying innovative treatment strategies to counter its diffusion. Pathogen infection prevention is among the most effective strategies to prevent the spread of both disease and AMR. Since their discovery, vaccines have been the strongest prophylactic weapon against infectious diseases, with a multitude of different antigen types and formulative strategies developed over more than a century to protect populations from different pathogens. In this review, we review the main characteristics of vaccine formulations in use and under development against AMR pathogens, focusing on the importance of administering multiple antigens where possible, and the challenges associated with their development and production. The most relevant antigen classes and adjuvant systems are described, highlighting their mechanisms of action and presenting examples of their use in clinical trials against AMR. We also present an overview of the analytical and formulative strategies for multivalent vaccines, in which we discuss the complexities associated with mixing multiple components in a single formulation. This review emphasizes the importance of combining existing knowledge with advanced technologies within a Quality by Design development framework to efficiently develop vaccines against AMR pathogens.
Differentially Expressed Genes Regulating Glutathione Metabolism, Protein-Folding, and Unfolded Protein Response in Pancreatic β-Cells in Type 2 Diabetes Mellitus
Authors: Klyosova Elena , Azarova Iuliia , Buikin Stepan , Polonikov Alexey
Published: July 2023

Impaired redox homeostasis in the endoplasmic reticulum (ER) may contribute to proinsulin misfolding and thus to activate the unfolded protein response (UPR) and apoptotic pathways, culminating in pancreatic β-cell loss and type 2 diabetes (T2D). The present study was designed to identify differentially expressed genes (DEGs) encoding enzymes for glutathione metabolism and their impact on the expression levels of genes regulating protein folding and UPR in β-cells of T2D patients. The GEO transcriptome datasets of β-cells of diabetics and non-diabetics, GSE20966 and GSE81608, were analyzed for 142 genes of interest using limma and GREIN software, respectively. Diabetic β-cells showed dataset-specific patterns of DEGs (FDR ≤ 0.05) implicated in the regulation of glutathione metabolism (ANPEP, PGD, IDH2, and CTH), protein-folding (HSP90AB1, HSP90AA1, HSPA1B, HSPA8, BAG3, NDC1, NUP160, RLN1, and RPS19BP1), and unfolded protein response (CREB3L4, ERP27, and BID). The GCLC gene, encoding the catalytic subunit of glutamate–cysteine ligase, the first rate-limiting enzyme of glutathione biosynthesis, was moderately down-regulated in diabetic β-cells from both datasets (p ≤ 0.05). Regression analysis established that genes involved in the de novo synthesis of glutathione, GCLC, GCLM, and GSS affect the expression levels of genes encoding molecular chaperones and those involved in the UPR pathway. This study showed for the first time that diabetic β-cells exhibit alterations in the expression of genes regulating glutathione metabolism, protein-folding, and UPR and provided evidence for the molecular crosstalk between impaired redox homeostasis and abnormal protein folding, underlying ER stress in type 2 diabetes.
The Protective Role of Glutathione against Doxorubicin-Induced Cardiotoxicity in Human Cardiac Progenitor Cells
Authors: Lee Eun Ji , Jang Woong Bi , Choi Jaewoo , Lim Hye Ji , Park Sangmi , Rethineswaran Vinoth Kumar , Ha Jong Seong , Yun Jisoo , Hong Young Joon , Choi Young Jin , Kwon Sang-Mo
Published: July 2023

This study investigated the protective effect of glutathione (GSH), an antioxidant drug, against doxorubicin (DOX)-induced cardiotoxicity. Human cardiac progenitor cells (hCPCs) treated with DOX (250 to 500 nM) showed increased viability and reduced ROS generation and apoptosis with GSH treatment (0.1 to 1 mM) for 24 h. In contrast to the 500 nM DOX group, pERK levels were restored in the group co-treated with GSH and suppression of ERK signaling improved hCPCs’ survival. Similarly to the previous results, the reduced potency of hCPCs in the 100 nM DOX group, which did not affect cell viability, was ameliorated by co-treatment with GSH (0.1 to 1 mM). Furthermore, GSH was protected against DOX-induced cardiotoxicity in the in vivo model (DOX 20 mg/kg, GSH 100 mg/kg). These results suggest that GSH is a potential therapeutic strategy for DOX-induced cardiotoxicity, which performs its function via ROS reduction and pERK signal regulation.
Thyroid Hormone Regulates the Lipid Content of Muscle Fibers, Thus Affecting Physical Exercise Performance
Authors: Miro Caterina , Nappi Annarita , Sagliocchi Serena , Di Cicco Emery , Murolo Melania , Torabinejad Sepehr , Acampora Lucia , Pastore Arianna , Luciano Paolo , La Civita Evelina , Terracciano Daniela , Stornaiuolo Mariano , Dentice Monica , Cicatiello Annunziata Gaetana
Published: July 2023

Skeletal muscle (SkM) lipid composition plays an essential role in physiological muscle maintenance and exercise performance. Thyroid hormones (THs) regulate muscle formation and fuel energy utilization by modulating carbohydrates and lipid and protein metabolism. The best-known effects of THs in SkM include the promotion of mitochondrial biogenesis, the fiber-type switch from oxidative to glycolytic fibers, and enhanced angiogenesis. To assess the role of THs on the lipidic composition of SkM fibers, we performed lipidomic analyses of SkM cells and tissues, glucose tolerance experiments, and exercise performance tests. Our data demonstrated that TH treatment induces remodeling of the lipid profile and changes the proportion of fatty acids in SkM. In brief, THs significantly reduced the ratio of stearic/oleic acid in the muscle similar to what is induced by physical activity. The increased proportion of unsaturated fatty acids was linked to an improvement in insulin sensitivity and endurance exercise. These findings point to THs as critical endocrine factors affecting exercise performance and indicate that homeostatic maintenance of TH signals, by improving cell permeability and receptor stability at the cell membrane, is crucial for muscle physiology.
The Effect of Cerebrolysin in an Animal Model of Forebrain Ischemic-Reperfusion Injury: New Insights into the Activation of the Keap1/Nrf2/Antioxidant Signaling Pathway
Authors: Marghani Basma H. , Rezk Shaymaa , Ateya Ahmed I. , Alotaibi Badriyah S. , Othman Basma H. , Sayed Samy M. , Alshehri Mohammed Ali , Shukry Mustafa , Mansour Mohamed M.
Published: July 2023

Forebrain ischemia-reperfusion (IR) injury causes neurological impairments due to decreased cerebral autoregulation, hypoperfusion, and edema in the hours to days following the restoration of spontaneous circulation. This study aimed to examine the protective and/or therapeutic effects of cerebrolysin (CBL) in managing forebrain IR injury and any probable underlying mechanisms. To study the contribution of reperfusion to forebrain injury, we developed a transient dual carotid artery ligation (tDCAL/IR) mouse model. Five equal groups of six BLC57 mice were created: Group 1: control group (no surgery was performed); Group 2: sham surgery (surgery was performed without IR); Group 3: tDCAL/IR (surgery with IR via permanently ligating the left CA and temporarily closing the right CA for 30 min, followed by reperfusion for 72 h); Group 4: CBL + tDCAL/IR (CBL was given intravenously at a 60 mg/kg BW dose 30 min before IR); and Group 5: tDCAL/IR + CBL (CBL was administered i.v. at 60 mg/kg BW three hours after IR). At 72 h following IR, the mice were euthanized. CBL administration 3 h after IR improved neurological functional recovery, enhanced anti-inflammatory and antioxidant activities, alleviated apoptotic neuronal death, and inhibited reactive microglial and astrocyte activation, resulting in neuroprotection after IR injury in the tDCAL/IR + CBL mice group as compared to the other groups. Furthermore, CBL reduced the TLRs/NF-kB/cytokines while activating the Keap1/Nrf2/antioxidant signaling pathway. These results indicate that CBL may improve neurologic function in mice following IR.
Potential Role of Moesin in Regulating Mast Cell Secretion
Authors: Theoharides Theoharis C. , Kempuraj Duraisamy
Published: July 2023

Mast cells have existed for millions of years in species that never suffer from allergic reactions. Hence, in addition to allergies, mast cells can play a critical role in homeostasis and inflammation via secretion of numerous vasoactive, pro-inflammatory and neuro-sensitizing mediators. Secretion may utilize different modes that involve the cytoskeleton, but our understanding of the molecular mechanisms regulating secretion is still not well understood. The Ezrin/Radixin/Moesin (ERM) family of proteins is involved in linking cell surface-initiated signaling to the actin cytoskeleton. However, how ERMs may regulate secretion from mast cells is still poorly understood. ERMs contain two functional domains connected through a long α-helix region, the N-terminal FERM (band 4.1 protein-ERM) domain and the C-terminal ERM association domain (C-ERMAD). The FERM domain and the C-ERMAD can bind to each other in a head-to-tail manner, leading to a closed/inactive conformation. Typically, phosphorylation on the C-terminus Thr has been associated with the activation of ERMs, including secretion from macrophages and platelets. It has previously been shown that the ability of the so-called mast cell “stabilizer” disodium cromoglycate (cromolyn) to inhibit secretion from rat mast cells closely paralleled the phosphorylation of a 78 kDa protein, which was subsequently shown to be moesin, a member of ERMs. Interestingly, the phosphorylation of moesin during the inhibition of mast cell secretion was on the N-terminal Ser56/74 and Thr66 residues. This phosphorylation pattern could lock moesin in its inactive state and render it inaccessible to binding to the Soluble NSF attachment protein receptors (SNAREs) and synaptosomal-associated proteins (SNAPs) critical for exocytosis. Using confocal microscopic imaging, we showed moesin was found to colocalize with actin and cluster around secretory granules during inhibition of secretion. In conclusion, the phosphorylation pattern and localization of moesin may be important in the regulation of mast cell secretion and could be targeted for the development of effective inhibitors of secretion of allergic and inflammatory mediators from mast cells.
Evaluation of Immune Infiltrates in Ovarian Endometriosis and Endometriosis-Associated Ovarian Cancer: Relationship with Histological and Clinical Features
Authors: Spagnolo Emanuela , Martinez Alejandra , Mascarós-Martínez Andrea , Marí-Alexandre Josep , Carbonell María , González-Cantó Eva , Pena-Burgos Eva Manuela , Mc Cormack Bárbara Andrea , Tomás-Pérez Sarai , Gilabert-Estellés Juan , López-Carrasco Ana , Hidalgo Paula , Ángeles Martina Aida , Redondo Andrés , Gallego Alejandro , Hernández Alicia
Published: July 2023

Background: the association between ovarian endometriosis (OE) and endometriosis-associated ovarian cancer (EAOC) is extensively documented, and misfunction of the immune system might be involved. The primary objective of this study was to identify and compare the spatial distribution of tumour-infiltrating lymphocytes (TILs) and tumour-associated macrophages (TAMs) in OE and EAOC. Secondary objectives included the analysis of the relationship between immunosuppressive populations and T-cell exhaustion markers in both groups. Methods: TILs (CD3, CD4, and CD8) and macrophages (CD163) were assessed by immunochemistry. Exhaustion markers (PD-1, TIM3, CD39, and FOXP3) and their relationship with tumour-associated macrophages (CD163) were assessed by immunofluorescence on paraffin-embedded samples from n = 43 OE and n = 54 EAOC patients. Results: we observed a predominantly intraepithelial CD3+ distribution in OE but both an intraepithelial and stromal pattern in EAOC (p < 0.001). TILs were more abundant in OE (p < 0.001), but higher TILs significantly correlated with a longer overall survival and disease-free survival in EAOC (p < 0.05). CD39 and FOXP3 significantly correlated with each other and CD163 (p < 0.05) at the epithelial level in moderate/intense CD4 EAOC, whereas in moderate/intense CD8+, PD-1+ and TIM3+ significantly correlated (p = 0.009). Finally, T-cell exhaustion markers FOXP3-CD39 were decreased and PD-1-TIM3 were significantly increased in EAOC (p < 0.05). Conclusions: the dysregulation of TILs, TAMs, and T-cell exhaustion might play a role in the malignization of OE to EAOC.
From Reductionistic Approach to Systems Immunology Approach for the Understanding of Tumor Microenvironment
Authors: Koelsch Nicholas , Manjili Masoud H.
Published: July 2023

The tumor microenvironment (TME) is a complex and dynamic ecosystem that includes a variety of immune cells mutually interacting with tumor cells, structural/stromal cells, and each other. The immune cells in the TME can have dual functions as pro-tumorigenic and anti-tumorigenic. To understand such paradoxical functions, the reductionistic approach classifies the immune cells into pro- and anti-tumor cells and suggests the therapeutic blockade of the pro-tumor and induction of the anti-tumor immune cells. This strategy has proven to be partially effective in prolonging patients’ survival only in a fraction of patients without offering a cancer cure. Recent advances in multi-omics allow taking systems immunology approach. This essay discusses how a systems immunology approach could revolutionize our understanding of the TME by suggesting that internetwork interactions of the immune cell types create distinct collective functions independent of the function of each cellular constituent. Such collective function can be understood by the discovery of the immunological patterns in the TME and may be modulated as a therapeutic means for immunotherapy of cancer.
Dysregulations of Key Regulators of Angiogenesis and Inflammation in Abdominal Aortic Aneurysm
Authors: Zalewski Daniel , Chmiel Paulina , Kołodziej Przemysław , Borowski Grzegorz , Feldo Marcin , Kocki Janusz , Bogucka-Kocka Anna
Published: July 2023

Abdominal aortic aneurysm (AAA) is a chronic vascular disease caused by localized weakening and broadening of the abdominal aorta. AAA is a clearly underdiagnosed disease and is burdened with a high mortality rate (65–85%) from AAA rupture. Studies indicate that abnormal regulation of angiogenesis and inflammation contributes to progression and onset of this disease; however, dysregulations in the molecular pathways associated with this disease are not yet fully explained. Therefore, in our study, we aimed to identify dysregulations in the key regulators of angiogenesis and inflammation in patients with AAA in peripheral blood mononuclear cells (using qPCR) and plasma samples (using ELISA). Expression levels of ANGPT1, CXCL8, PDGFA, TGFB1, VEGFB, and VEGFC and plasma levels of TGF-alpha, TGF-beta 1, VEGF-A, and VEGF-C were found to be significantly altered in the AAA group compared to the control subjects without AAA. Associations between analyzed factors and risk factors or biochemical parameters were also explored. Any of the analyzed factors was associated with the size of the aneurysm. The presented study identified dysregulations in key angiogenesis- and inflammation-related factors potentially involved in AAA formation, giving new insight into the molecular pathways involved in the development of this disease and providing candidates for biomarkers that could serve as diagnostic or therapeutic targets.
A Surface-Enhanced Raman Spectroscopy-Based Biosensor for the Detection of Biological Macromolecules: The Case of the Lipopolysaccharide Endotoxin Molecules
Authors: Rusciano Giulia , Capaccio Angela , Sasso Antonio , Capo Alessandro , Almuzara Carlos Murillo , Staiano Maria , D’Auria Sabato , Varriale Antonio
Published: July 2023

The development of sensitive methods for the detection of endotoxin molecules, such as lipopolysaccharides (LPS), is essential for food safety and health control. Conventional analytical methods used for LPS detection are based on the pyrogen test, plating and culture-based methods, and the limulus amoebocyte lysate method (LAL). Alternatively, the development of reliable biosensors for LPS detection would be highly desirable to solve some critical issues, such as high cost and a long turnaround time. In this work, we present a label-free Surface-Enhanced Raman Spectroscopy (SERS)-based method for LPS detection in its free form. The proposed method combines the benefits of plasmonic enhancement with the selectivity provided by a specific anti-lipid A antibody (Ab). A high-enhancing nanostructured silver substrate was coated with Ab. The presence of LPS was quantitatively monitored by analyzing the changes in the Ab spectra obtained in the absence and presence of LPS. A limit of detection (LOD) and quantification (LOQ) of 12 ng/mL and 41 ng/mL were estimated, respectively. Importantly, the proposed technology could be easily expanded for the determination of other biological macromolecules.
New Ideas for the Prevention and Treatment of Preeclampsia and Their Molecular Inspirations
Authors: Sakowicz Agata , Bralewska Michalina , Rybak-Krzyszkowska Magda , Grzesiak Mariusz , Pietrucha Tadeusz
Published: July 2023

Preeclampsia (PE) is a pregnancy-specific disorder affecting 4–10% of all expectant women. It greatly increases the risk of maternal and foetal death. Although the main symptoms generally appear after week 20 of gestation, scientific studies indicate that the mechanism underpinning PE is initiated at the beginning of gestation. It is known that the pathomechanism of preeclampsia is strongly related to inflammation and oxidative stress, which influence placentation and provoke endothelial dysfunction in the mother. However, as of yet, no “key players” regulating all these processes have been discovered. This might be why current therapeutic strategies intended for prevention or treatment are not fully effective, and the only effective method to stop the disease is the premature induction of delivery, mostly by caesarean section. Therefore, there is a need for further research into new pharmacological strategies for the treatment and prevention of preeclampsia. This review presents new preventive methods and therapies for PE not yet recommended by obstetrical and gynaecological societies. As many of these therapies are in preclinical studies or under evaluation in clinical trials, this paper reports the molecular targets of the tested agents or methods.
The Effect of Empowerment on the Adoption of Soil and Water Conservation Technology in the Loess Plateau of China
Authors: Ren Yaqin , Feng Hui , Gao Tianzhi
Published: July 2023

Since there is a serious problem of land degradation and soil erosion in the Loess Plateau region of China, soil and water conservation technology (SWC) is necessary to protect land resources and reduce water loss, so as to promote the development of sustainable agriculture. By using the binary probit model, this study analyzes the effect of organizational, economic and self-empowerment on the adoption of soil and water conservation technology in the Loess Plateau region of China. The results indicated that all these empowerments have significant positive effects on the adoption of SWC, and there are significant gender differences in the impact. Organizational empowerment had a more significant impact on the adoption of SWC by male household heads, while economic empowerment and self-empowerment had a more significant effect on the adoption of SWC by female household heads. Therefore, more organizational and financial support for farmers is needed, and farmers themselves should have more confidence in the adoption of new technologies. Furthermore, given the gender differences in the impact, more gender equality should be ensured in the promotion of agricultural technologies.
Enhanced Catalytic Activity and Energy Savings with Ni-Zn-Mo Ionic Activators for Hydrogen Evolution in Alkaline Electrolysis
Authors: Perović Ivana , Marčeta Kaninski Milica , Tasić Gvozden , Maslovara Sladjana , Laušević Petar , Seović Mina , Nikolić Vladimir
Published: July 2023

Green hydrogen produced by alkaline electrolysis is a promising solution to address the world’s increasing energy demand while mitigating greenhouse gas emissions. However, the efficient and cost-effective production of green hydrogen via alkaline electrolysis requires improvements. This paper presents an in situ activation process that simplifies the alkaline electrolysis technology while enhancing the catalytic activity of electrodes for the hydrogen evolution reaction. The aim of this research is to enhance the energy efficiency of alkaline electrolysis and decrease the energy consumption for hydrogen production. To achieve this goal, ionic activators comprising Ni, Zn, and Mo were incorporated into the standard electrolyte solution. Our results demonstrate that the anticipated improvement in the catalytic activity of the d-metal combination, surpassing even that of precious metals, has been successfully attained. As a result, a 20% reduction in energy consumption (REC) for the hydrogen produced has been observed. The catalytic activity of the added activators for the hydrogen evolution reaction was discussed by analyzing the mechanism of the reaction via Tafel analysis and EIS techniques. These findings offer a promising approach to improve alkaline electrolysis and enhance the production of green hydrogen.
Influence of Multiple Used Implant Drills on Their Cutting Performance and Fracture Resistance
Authors: Alevizakos Vasilios , Mosch Richard , von See Constantin
Published: July 2023

This study aimed to analyze the influence of multiple uses of zirconia implant drills on their cutting performance and bending strength. The hypothesis was that drill usage and sterilization cycles would not affect drilling time or flexural strength. Sixty zirconia twist drills from Z-Systems were used to drill in the angulus mandibulae region of fresh porcine jaws. The drills were divided into four groups based on the cycle count, and the drilling time was measured. Bending strength tests were conducted using a universal testing machine, and statistical analysis was performed using ANOVA tests. The results showed that drilling times followed a normal distribution, and significant differences were observed in drilling times between group 1 and the other groups for the pilot drill. However, no significant differences were found for ø3.75 mm and ø4.25 mm drills, and drilling times also varied significantly among different drill diameters, regardless of the cycle count. Flexural strength did not significantly differ among drill diameters or sterilization cycles. Overall, using and sterilizing zirconia implant drills had no significant impact on drilling time or flexural strength. Nevertheless, drilling times did vary depending on the diameter of the drill. These findings provide valuable insights into the performance and durability of zirconia implant drills, contributing to the optimization of dental implant procedures.
Drop-Dry Deposition of SnO2 Using a Complexing Agent and Fabrication of Heterojunctions with Co3O4
Authors: Li Tong , Ichimura Masaya
Published: July 2023

The drop-dry deposition (DDD) is a simple chemical technique of thin film deposition, which can be applied to metal oxides. The deposition solution is an aqueous solution including a metal salt and an alkali. However, some metal ions react spontaneously with water and precipitate. This work is the first attempt to use complexing agents in DDD to suppress the precipitation. SnO2 thin films are fabricated using DDD with Na2S2O3 as a complexing agent and via annealing in air. The results of the Auger electron spectroscopy measurement show that the O/Sn composition ratio of the annealed films approached two, indicating that the annealed films are SnO2. The photoelectrochemical measurement results show that the annealed films are n-type. Co3O4/SnO2 heterojunction is fabricated using p-type Co3O4 films which are also deposited via DDD. The heterojunction has rectification and photovoltaic properties. Thus, for the first time, a metal oxide thin film was successfully prepared via DDD using a complexing agent, and oxide thin film solar cells are successfully prepared using only DDD.
Magnetron Sputtering Deposition of High Quality Cs3Bi2I9 Perovskite Thin Films
Authors: Caporali Stefano , Martinuzzi Stefano Mauro , Gabellini Lapo , Calisi Nicola
Published: July 2023

Nontoxic all-inorganic perovskites are among the most promising materials for the realization of optoelectronic devices. Here, we present an innovative way to deposit lead-free, totally inorganic Cs3Bi2I9 perovskite from vapor phase. Taking use of a magnetron sputtering system equipped with a radiofrequency working mode power supply and a single target containing the correct ratio of CsI and BiI3 salts, it was possible to deposit a Cs3Bi2I9 perovskitic film on silicon and soda-lime glass. The target composition was optimized to obtain a stoichiometric deposition, and the best compromise was found with a mix enriched with 20% w/w of CsI. Secondly, the effect of post-deposition thermal treatments (150 °C and 300 °C) and of the deposition on a preheat substrate (150 °C) were evaluated by analyzing the chemical composition, the morphology, the crystal structure, and the optical properties. The thermal treatment at 150 °C improved the uniformity of the perovskite film; the one at 300 °C damaged the perovskite deposited. Depositing on a preheated substrate at 150 °C, the obtained film showed a higher crystallinity. An additional thermal treatment at 150 °C on the film deposed on the preheated substrate showed that the crystallinity remains high, and the morphology becomes more uniform.
Interlaminar Properties of Prepregs Reinforced with Multiwalled Carbon Nanotubes/Graphene Oxide
Authors: Wen Liwei , Shen Haiqing , Chen Zhuan
Published: July 2023

Carbon-fiber-reinforced polymer (CFRP) composites are widely used in industries such as aerospace due to their lightweight nature and high strength. However, weak interfacial bonding strength is one of the main problems of resin-based composites. In this study, a prepreg was prepared by melt mixing. By dispersing nanoreinforcement particles in the resin, the interlaminar shear strength of the CFRP was increased by approximately 23.6%. When only 0.5 wt% multiwalled carbon nanotube (MWCNT) was used for reinforcement, scanning electron microscopy (SEM) micrographs showed that cracks were hindered by the MWCNTs during propagation, causing crack deflection. At the same time, the mechanism of MWCNTs pulling out increased the energy required for crack propagation. When only 0.5 wt% graphene oxide (GO) was added, the reinforcement effect was inferior to that of using the same amount of MWCNTs. The laminar structure formed by GO and the resin matrix adhered to the carbon fiber surface, reducing the degree of destruction of the resin matrix, but its hindering effect on crack propagation was weak. When 0.5 wt% of MWCNT and GO mixture was added, the interlayer shear strength increased from 55.6 MPa in the blank group to 68.7 MPa. The laminar structure of GO provided a platform for the MWCNTs to form a mesh structure inside its matrix. At the same time, the tubular structure of the MWCNTs inhibited the stacking of GO, providing better dispersion and forming a synergistic enhancement effect.
Optimization of Cutting Parameters for Deep Hole Boring of Ti-6Al-4V Deep Bottle Hole
Authors: Li Wanzhong , Zheng Huan , Feng Yazhou
Published: July 2023

In this study, the cutting parameters for machining deep bottle holes (deep holes with complex profiles and length-to-diameter ratio greater than 10) were optimized based on cutting simulation, a regression analysis genetic algorithm, and experimental validation. The influence of cutting parameters on cutting force and cutting temperature was analyzed using the response surface method (RSM), and the regression prediction model of cutting parameters with cutting force and most cutting temperature was established. Based on this model, multi-objective optimization of cutting force Fx and material removal rate Q was carried out based on a genetic algorithm, and a set of optimal cutting parameters (v = 139.41 m/min, ap = 1.12 mm, f = 0.27 mm/rev) with low cutting force and high material removal rate were obtained. Finally, based on the optimal cutting parameters, the machining of TC4 deep bottle holes with a length-to-diameter (L/D) ratio of 36.36 and a roughness of Ra 3.2 µm was accomplished through a deep hole boring experiment, which verified the feasibility of the selected cutting parameters and provided a certain reference for the machining of this type of parts.
The Influence of Fiber on the Mechanical Properties of Controllable Low-Strength Materials
Authors: Qian Yafeng , Jiang Mingyang
Published: July 2023

Numerous studies have been conducted on fiber-reinforced concrete; however, comparative investigations specifically focusing on the utilization of fibers in CLSM remain limited. In this study, we conducted a systematic investigation into the mechanical properties of controlled low-strength material (CLSM) by manipulating the length and doping amount of fibers as control variables. The 7-day compressive strength (7d-UCS), 28-day compressive strength (28d-UCS), and 28-day splitting strength of CLSM were employed as indicators to evaluate the material’s performance. Based on our comprehensive analysis, the following conclusions were drawn: (1) A positive correlation was observed between fiber length and material strength within the range of 0–6 mm, while conversely, a negative correlation was evident. Similarly, when the fiber doping was within the range of 0–0.3%, a positive correlation was identified between material strength and fiber doping. However, the strength of CLSM decreased when fiber doping exceeded 0.3%. (2) SEM and PCAS analyses provided further confirmation that the incorporation of fibers effectively reduced the porosity of the material by filling internal pores and interacting with hydration products, thereby forming a mesh structure. Overall, this study offers valuable insights into the manipulation of fiber length and doping amount to optimize the mechanical properties of CLSM. The findings have important implications for the practical application of CLSM, particularly in terms of enhancing its strength through fiber incorporation.
Effects of Polyethylene Glycol/Porous Silica Form-Stabilized Phase Change Materials on the Performance of Asphalt Binders
Authors: Wang Hao , Pan Gui , He Lihong , Zou Ling
Published: July 2023

The road performance and temperature-regulating properties of asphalt binders modified with novel polyethylene glycol (PEG)/porous silica (PS) form-stabilized phase-change materials (PEG/PS-fs-PCMs) were studied. PS and PEG were used as the supporting substance and PCMs. The results showed that PEG/PS-fs-PCMs could maintain a maximum weight percentage of 70% without leakage, at temperatures as high as 90 °C. The PEG/PS-fs-PCMs exhibited stable chemical structures, excellent thermal stability, high heat storage density, and suitable phase-change temperature. Based on conventional physical tests, the addition of PEG/PS-fs-PCMs can increase the viscosity and the degree of hardness of asphalt binders; thus, achieving an excellent comprehensive performance of the modified asphalt binder depends on determining the optimal dosage of PEG/PS-fs-PCMs. Additionally, incorporating PEG/PS-fs-PCM particles into the asphalt binder can enhance its ability to withstand permanent deformation at elevated temperatures, while PEG/PS-fs-PCMs mainly act as a filler, weakening the cohesive force of the asphalt molecules, and preventing the ductility of asphalt from expansion, according to DSR and BBR tests. Moreover, the use of PEG/PS-fs-PCMs can enhance the heat transfer properties of the asphalt binders, resulting in an improved temperature regulation performance. However, the accumulation of PEG/PS-fs-PCM particles on asphalt binders can negatively impact the storage stability of the modified asphalt binders, because of the difference in density between the two materials.
Time-Dependent Rheological Properties of Cemented Aeolian Sand-Fly Ash Backfill Vary with Particles Size and Plasticizer
Authors: Yang Baogui , Zheng Zhijun , Jin Junyu , Wang Xiaolong
Published: July 2023

The use of cemented Aeolian sand-fly ash backfill (CAFB) material to fill the mining area to improve the surface subsidence damage caused by underground coal mining is in the development stage. Their performance with large overflow water and strength loss is not well understood. Few research has been conducted to understand the effects of aeolian sand and coal gangue on the rheological properties of CAFB with plasticizers. Therefore, this study aims to investigate the effects of a plasticizer on the rheological properties, specifically yield stress and viscosity, of CAFB prepared with aeolian sand and coal gangue. CAFB mixes containing 0%, 0.05%, and 0.1% plasticizers were prepared, and yield stress and viscosity were determined at different intervals. Additional tests, such as thermal analysis and zeta potential analysis, were also conducted. It was found that the rheological properties of CAFB are the comprehensive manifestation of the composite characteristics of various models. Reasonable particle size distribution and less plasticizer can ensure the stability of the slurry structure and reduce the slurry settlement and the risk of pipe blocking. The findings of this study will be beneficial in the design and production of CAFB material.
Reduction of Friction and Wear for AISI321 Stainless Steel through Surface Modification Using Nanocrystallization
Authors: Ding Lifen , Li You
Published: July 2023

Through surface nanocrystallization and low-temperature ion sulfurization, the nanocrystalline/FeS thin film with excellent friction-reduction and antiwear properties was fabricated on the surface of AISI321 stainless steel. The nanocrystallization treatment formed the high hardness and active nanocrystalline structure on the surface of AISI321, with the harness increased from 4.6 GPa to 7.56 GPa. Furthermore, the significantly refined nanostructure strongly increased the concentration of S element in comparison with the single-sulfurized layer on the substrate. Tribological tests reveal that both the original AISI321 substrate and the single-sulfurizing-treated samples are subject to severe abrasion. Single nanocrystallization treatment can improve the wear resistance of AISI321, while the compound treatment can obviously improve the comprehensive tribological properties. The compound-modified layer presents excellent tribological properties with the lowest coefficient of friction (COF) of 0.33, which is related to the increased hardness of the substrate and increased thickness, density, and homogeneity of the sulfurized layer. Furthermore, a physical model is developed for the vacuum tribological behavior of the samples after different treatments. This model provides a reference for revealing the tribological mechanism of the compound-modified layer treated using surface nanocrystallization-assisted chemical heat treatment.
The Effect of Sterilization on the Accuracy and Fit of 3D-Printed Surgical Guides
Authors: Yazigi Christine , Chaar M. Sad , Busch Reinhard , Kern Matthias
Published: July 2023

This study was conducted to evaluate the accuracy of 3D-printed surgical guides before and after sterilization in a steam sterilizer. A test-model incorporating three implant replicas was customized. A total of forty guides were printed from five printable resins. A group made from a self-curing composite served as control group. The guides were checked for fit. Vertical discrepancies between the model and guides were measured at standardized points at a load of 500 g (P1). The guides were connected to implant replicas and scanned, and their angles were digitally measured. The specimens were sterilized in a steam sterilizer at 121 °C for 20 min at 2 bar pressure. Vertical discrepancies (P2) and angulations were remeasured. Additionally, the specimens were repositioned with an increased load, and measurements were repeated (P3). All specimens were repositionable after sterilization. The smallest variation in discrepancy at a 500 g load was 428 µm, whereas the greatest was 1487 µm. Under an increased force, the smallest change was 94 µm, while the greatest was 260 µm. The level of significance α = 0.05 (95% confidence interval) was set for all tests. The variation in the measured angles was not statistically significant (Kruskal–Wallis’s test, p > 0.05). The accuracy was affected by the material and sterilization, but it was clinically acceptable when an increased load was applied during repositioning.
Iron Selenide Particles for High-Performance Supercapacitors
Authors: Scarpa Davide , Cirillo Claudia , Ponticorvo Eleonora , Cirillo Carla , Attanasio Carmine , Iuliano Mariagrazia , Sarno Maria
Published: July 2023

Nowadays, iron (II) selenide (FeSe), which has been widely studied for years to unveil the high-temperature superconductivity in iron-based superconductors, is drawing increasing attention in the electrical energy storage (EES) field as a supercapacitor electrode because of its many advantages. In this study, very small FeSe particles were synthesized via a simple, low-cost, easily scalable, and reproducible solvothermal method. The FeSe particles were characterized using cyclic voltammetry (CV), galvanostatic charge/discharge (GCD) measurements, and electrochemical impedance spectroscopy (EIS), revealing enhanced electrochemical properties: a high capacitance of 280 F/g at 0.5 A/g, a rather high energy density of 39 Wh/kg and a corresponding power density of 306 W/kg at 0.5 A/g, an extremely high cycling stability (capacitance retention of 92% after 30,000 cycles at 1 A/g), and a rather low equivalent series resistance (RESR) of ~2 Ω.
Determining Homogenization Parameters and Predicting 5182-Sc-Zr Alloy Properties by Artificial Neural Networks
Authors: Li Jingxiao , Du Dongfang , Yang Xiaofang , Qiu Youcai , Xiang Shihua
Published: July 2023

Artificial neural networks (ANNs) were established for the homogenization and recrystallization heat treatment processes of 5182-Sc-Zr alloy. Microhardness and conductivity testing were utilized to determine the precipitation state of Al3(ScxZr1−x) dispersoids during the homogenization treatment, while electron backscatter diffraction (EBSD) and transmission electron microscopy (TEM) were used to observe the microstructure evolution of the alloy. Tensile experiments were performed to test the mechanical properties of the alloy after recrystallization annealing. The two-stage homogenization parameters were determined by studying the changes in microhardness and electrical conductivity of 5182-Sc-Zr alloy after homogenization with the assistance of artificial neural networks: the first-stage homogenization at 275 °C for 20 h and the second-stage homogenization at 440 °C for 12 h. The dispersoids had entirely precipitated after homogenization, and the alloy segregation had improved. A high-accuracy prediction model, incorporating multiple influencing factors through artificial neural networks, was successfully established to predict the mechanical properties of the 5182-Sc-Zr alloy after annealing. Based on the atomic plane spacing in HRTEM, it was determined that the Al3(ScxZr1−x) dispersoids and the Al matrix maintained a good coherence relationship after annealing at 400 °C.
Properties of SiCN Films Relevant to Dental Implant Applications
Authors: Xia Xinyi , Chiang Chao-Ching , Gopalakrishnan Sarathy K. , Kulkarni Aniruddha V. , Ren Fan , Ziegler Kirk J. , Esquivel-Upshaw Josephine F.
Published: July 2023

The application of surface coatings is a popular technique to improve the performance of materials used for medical and dental implants. Ternary silicon carbon nitride (SiCN), obtained by introducing nitrogen into SiC, has attracted significant interest due to its potential advantages. This study investigated the properties of SiCN films deposited via PECVD for dental implant coatings. Chemical composition, optical, and tribological properties were analyzed by adjusting the gas flow rates of NH3, CH4, and SiH4. The results indicated that an increase in the NH3 flow rate led to higher deposition rates, scaling from 5.7 nm/min at an NH3 flow rate of 2 sccm to 7 nm/min at an NH3 flow rate of 8 sccm. Concurrently, the formation of N-Si bonds was observed. The films with a higher nitrogen content exhibited lower refractive indices, diminishing from 2.5 to 2.3 as the NH3 flow rate increased from 2 sccm to 8 sccm. The contact angle of SiCN films had minimal differences, while the corrosion rate was dependent on the pH of the environment. These findings contribute to a better understanding of the properties and potential applications of SiCN films for use in dental implants.