bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Planar Ultra-Wideband Modular Antenna Array with Low Profile
Authors: Ye Yuan , Wang Shaozhi , Liu Boyuan , Wang Qingping , Zhou Yang , Huang Jingjian , Chen Xi , Xue Quan , Yuan Naichang
Published: December 2022

A planar ultra-wideband modular antenna array with an ultra-low profile based on a tight coupling effect is proposed. The antenna array is composed of multi-layer printed circuit boards. Since its frequency operation can extend up to the grading lobe frequency, the number of T/R components for the antenna aperture is reduced to the greatest extent. Specifically, a horizontal “fin” structure is added on the parallel feeding lines, which assists in improving the impedance matching of the array. In order to break the bandwidth limitation of the low-frequency loop mode, a capacitive short-circuit probe is introduced to push the common-mode resonance point into the low-frequency band. Finally, subject to Active VSWR < 3, the array can realize E-plane, H-plane, and D-plane ± 45° beam scan coverage across the 6:1 frequency band (3.48–20.97 GHz). A 128-element prototype was processed and tested to validate the design. This array not only has the advantages of low profile, modularity, easy assembly, and maintenance but also minimizes the number of T/R components and reduces the cost of phased array antennas.
A Common-Ground-Type Five-Level Inverter with Dynamic Voltage Boost
Authors: Kakar Saifullah , Ayob Shahrin Md. , Lee Sze Sing , Arif M. Saad Bin , Nordin Norjulia Mohamad , Barzegarkhoo Reza , Siwakoti Yam P.
Published: December 2022

Today, transformerless inverters (TIs) are widely applicable in different solar photovoltaic (PV) grid-connected applications owing to their promising features, such as higher efficiency and power density. However, high-frequency common-mode voltage (CMV) in these topologies can result in high leakage current, electromagnetic interference, and lack of safety, reducing the whole system’s reliability. To resolve the problems associated with TIs, this paper proposes a novel hybrid switched capacitor (SC)-based common-ground (CG) transformerless inverter (TI) topology, which can be applied in grid-connected photovoltaic (PV) applications. The boost inductor is integrated to achieve continuous input current and dynamic voltage gain. In addition, the proposed circuit comprises nine switches and two SCs with a single input DC source. It can generate five-level AC voltage with voltage boosting within a single-stage DC–AC power conversion. The working principles of the proposed topology, circuit description, and control technique are presented. Furthermore, the proposed inverter is comprehensively compared with other five-level TIs to show its superiority. Finally, a laboratory prototype is developed and tested to validate the practical viability of the proposed configuration.
An Accurate Urine Red Blood Cell Detection Method Based on Multi-Focus Video Fusion and Deep Learning with Application to Diabetic Nephropathy Diagnosis
Authors: Hao Fang , Li Xinyu , Li Ming , Wu Yongfei , Zheng Wen
Published: December 2022

Background and Objective: Detecting urine red blood cells (U-RBCs) is an important operation in diagnosing nephropathy. Existing U-RBC detection methods usually employ single-focus images to implement such tasks, which inevitably results in false positives and missed detections due to the abundance of defocused U-RBCs in the single-focus images. Meanwhile, the current diabetic nephropathy diagnosis methods heavily rely on artificially setting a threshold to detect the U-RBC proportion, whose accuracy and robustness are still supposed to be improved. Methods: To overcome these limitations, a novel multi-focus video dataset in which the typical shape of all U-RBCs can be captured in one frame is constructed, and an accurate U-RBC detection method based on multi-focus video fusion (D-MVF) is presented. The proposed D-MVF method consists of multi-focus video fusion and detection stages. In the fusion stage, D-MVF first uses the frame-difference data of multi-focus video to separate the U-RBCs from the background. Then, a new key frame extraction method based on the three metrics of information entropy, edge gradient, and intensity contrast is proposed. This method is responsible for extracting the typical shapes of U-RBCs and fusing them into a single image. In the detection stage, D-MVF utilizes the high-performance deep learning model YOLOv4 to rapidly and accurately detect U-RBCs based on the fused image. In addition, based on U-RBC detection results from D-MVF, this paper applies the K-nearest neighbor (KNN) method to replace artificial threshold setting for achieving more accurate diabetic nephropathy diagnosis. Results: A series of controlled experiments are conducted on the self-constructed dataset containing 887 multi-focus videos, and the experimental results show that the proposed D-MVF obtains a satisfactory mean average precision (mAP) of 0.915, which is significantly higher than that of the existing method based on single-focus images (0.700). Meanwhile, the diabetic nephropathy diagnosis accuracy and specificity of KNN reach 0.781 and 0.793, respectively, which significantly exceed the traditional threshold method (0.719 and 0.759). Conclusions: The research in this paper intelligently assists microscopists to complete U-RBC detection and diabetic nephropathy diagnosis. Therefore, the work load of microscopists can be effectively relieved, and the urine test demands of nephrotic patients can be met.
Robotic Manipulation in the Ceramic Industry
Authors: Torres Rogério , Ferreira Nuno
Published: December 2022

Robotic manipulation, an area inside the field of industrial automation and robotics, consists of using a robotic arm to guide and grasp a desired object through actuators such as a vacuum or fingers, among others. Some objects, such as fragile ceramic pieces, require special attention to the force and the gripping method exerted on them. For this purpose, two grippers were developed, where one of them is a rotary vacuum gripper and the other is an impact gripper with three fingers, each one equipped with a load sensor capable of evaluating the values of load exerted by the grip actuators onto the object to be manipulated. The vacuum gripper was developed for the purpose of glazing a coffee saucer and the gripper with three fingers was developed for the purpose of polishing a coffee cup. Being that the impact gripper with sensorial feedback reacts to the excess and lack of grip force exerted, both these grippers were developed with success, handling with ease the ceramic pieces proposed.
Producing Refuse Derived Fuel with Refining Industry Oily Sludge and Mushroom Substrates
Authors: Lee Chien Li , Jou Chih-Ju G.
Published: December 2022

The sludge in this study was obtained from refinery crude oil storage tanks. It contained a high proportion of hydrocarbon composition and harmful substances (such as polycyclic aromatic hydrocarbons and benzene). Through the microwave irradiation treatment process, the harmful substances were removed from the sludge which was then recycled and combined with agricultural waste mushroom substrates to produce refuse derived fuel (RDF). The results showed that the calorific value of RDF was 7279 cal/g when the blending ratio (wt/wt) of oil sludge and mushroom substrates was 5:5. On the other hand, when the portion of the mushroom substrates was increased, the sludge became easier to ignite with better combustion reaction. When the blending ratio (wt/wt) was changed from 8:2 to 5:5, the ignition index and comprehensive performance index were increased by 51.9 and 50.2%. Therefore, mixing the sludge with agricultural waste mushroom substrates is in line with the concept of waste recycling and circular economy.
Stem Taper Estimation Using Artificial Neural Networks for Nothofagus Trees in Natural Forest
Authors: Sandoval Simón , Acuña Eduardo
Published: December 2022

The objective of the study was to estimate the diameter at different stem heights and the tree volume of the Nothofagus obliqua (Mirb.) Oerst., Nothofagus alpine (Poepp. et Endl.) Oerst. and Nothofagus dombeyi (Mirb.) Oerst. trees using artificial neural networks (ANNs) and comparing the results with estimates obtained from six traditional taper functions. A total of 1380 trees were used. The ANN trained to estimate the stem diameter with the best performance generated RMSE values in the training phase of 7.5%, and 7.7% in the validation phase. Regarding taper functions, Kozak’s model generated better RMSE indicators, but performed not as well as that generated by the ANN. The ANN estimation of the total volume was carried out in two phases. The first used the diameter estimation to determine the volume at one-centimeter intervals along the stem (one-phase ANN), and the second used the estimation of the one-phase ANN as an additional variable in an ANN that directly estimated the tree cumulative volume (two-phase ANN). The two-phase ANN method generated the best performance for estimating the cumulative volume in relation to one-phase ANN and the Kozak taper function, generating RMSE values for N. obliqua, N. alpina and N. dombeyi of 9.7%, 8.9% and 8.8%, respectively.
Effective Fractal Dimension at 2d-3d Crossover
Authors: Chalyi Alexander V.
Published: December 2022

This article is aimed at reviewing and studying the effects of the 2d-3d crossover on the effective fractal and spatial dimensions, as well as on the critical exponents of the physical properties of bulk and bounded systems at criticality. Here we consider the following problems: (1) the two types of dimensional crossovers and the concept of the universality classes; (2) a smooth 2d-3d crossover and the calculation of the effective fractal and spatial dimensions, as well as the effective critical indices; (3) the fractal dimension, its connection with the random mean square order-parameter fluctuations and a new phase formation; (4) the fractal nuclei of a new phase and the medical consequences of carcinogenesis and nucleation isomorphism.
Genetic Diversity Analysis and Core Germplasm Collection Construction of Camellia oleifera Based on Fruit Phenotype and SSR Data
Authors: Zhu Yunzheng , Liang Deyang , Song Zejun , Tan Yi , Guo Xiaolan , Wang Delu
Published: December 2022

Many Camellia oleifera germplasm resources were collected from Guizhou Province, but the fruit morphological variation and genetic diversity of C. oleifera germplasm resources remain unclear. The genetic diversity of C. oleifera germplasms resources in Guizhou was studied based on fruit traits and simple sequence repeat (SSR) molecular markers to build a core collection. This paper aims to provide a scientific basis for the collection, management, development, and utilization of C. oleifera resources in Guizhou province. The variation coefficients among and within varieties of seven fruit phenotypic traits of C. oleifera ranged from 11.79% to 61.76% and from 8.15% to 42.31%, respectively, showing rich phenotypic variation. Furthermore, 12 SSR markers were used to analyze the genetic diversity. These primers generated 214 polymorphic bands, and the average number was 17.833. The average number of effective alleles (Ne), Shannon’s information index (I), observed heterozygosity (Ho), expected heterozygosity (He), polymorphic information content (PIC), and major allele frequency (MAF) were 8.999, 2.301, 0.965, 0.50, 0.836, and 0.238, respectively. The results showed that 12 SSR markers had high polymorphism, and the genetic diversity of 167 C. oleifera germplasm resources was high. Based on SSR molecular marker information and fruit traits clustering, 167 C. oleifera germplasm resources were divided into three groups. When constructing core collections based on fruit traits and molecular marker information, the PowerCore-25 of core collections greatly preserves fruit traits and improves genetic diversity. This paper can provide a reference for the genetic diversity and fruit traits variation of C. camellia germplasm resources in Guizhou Province. It is significant for establishing a core collection, thus promoting germplasm innovation and the development of the oil tea industry in Guizhou.
Paediatric Spinal Deformity Surgery: Complications and Their Management
Authors: Roberts Simon B. , Tsirikos Athanasios I.
Published: December 2022

Surgical correction of paediatric spinal deformity is associated with risks, adverse events, and complications that must be preoperatively discussed with patients and their families to inform treatment decisions, expectations, and long-term outcomes. The incidence of complications varies in relation to the underlying aetiology of spinal deformity and surgical procedure. Intraoperative complications include bleeding, neurological injury, and those related to positioning. Postoperative complications include persistent pain, surgical site infection, venous thromboembolism, pulmonary complications, superior mesenteric artery syndrome, and also pseudarthrosis and implant failure, proximal junctional kyphosis, crankshaft phenomenon, and adding-on deformity, which may necessitate revision surgery. Interventions included in enhanced recovery after surgery protocols may reduce the incidence of complications. Complications must be diagnosed, investigated and managed expeditiously to prevent further deterioration and to ensure optimal outcomes. This review summarises the complications associated with paediatric spinal deformity surgery and their management.
Opinions of Prospective Dentists and Prospective Teachers on Antibiotic Use
Authors: Gul Seyda , Gul Pinar
Published: December 2022

The purpose of this study was to examine the opinions of prospective dentists and prospective teachers on antibiotic use. In this study, a survey method based on a quantitative research paradigm was utilized. A 19-item instrument was applied to the sample group including 414 university students attending to the Faculty of Dentistry and Faculty of Education. The instrument was composed of three main subscales in addition to demographic characteristics. The first part focused on attitudes, the second part focused on subjective norms, and the third part focused on the prospective dentists and prospective teachers’ intentions regarding the phenomenon of antibiotics use. While the dependent variables of the study were the participants’ levels of agreement in the subscales of the instrument, the independent variable was faculty studied. The data were analyzed through descriptive statistics, and the Mann–Whitney U test was used to compare the participants’ views on antibiotic use. The findings in terms of the faculties showed that dentistry and education faculties in our country prepare well in expanding their reasonable consumption of antibiotics. Yet, antibiotics are used more incorrectly by some prospective teachers than by prospective dentists. According to the findings in terms of subscales, the participants have positive attitudes toward the reasonable use of antibiotics in terms of the attitude subscale. However, the results from the subjective norm indicated that participants are influenced by their environment in their antibiotic use. Finally, the results from the intention indicated that participants avoid using antibiotics irregularly or frequently.
Parametric Modeling Method for 3D Symbols of Fold Structures
Authors: Li An-Bo , Chen Hao , Du Xiao-Feng , Sun Guo-Kai , Liu Xian-Yu
Published: December 2022

Most fabrication methods for three-dimensional (3D) geological symbols are limited to two types: directly increasing the dimensionality of a 2D geological symbol or performing appropriate modeling for an actual 3D geological situation. The former can express limited vertical information and only applies to the three-dimensional symbol-making of point mineral symbols, while the latter weakens the difference between 3D symbols and 3D geological models and has several disadvantages, such as high dependence on measured data, redundant 3D symbol information, and low efficiency when displayed in a 3D scene. Generating a 3D geological symbol is represented by the process of constructing a 3D geological model. This study proposes a parametric modeling method for 3D fold symbols according to the complexity and diversity of the fold structures. The method involves: (1) obtaining the location of each cross-section in the symbol model, based on the location parameters; (2) constructing the middle cross-section, based on morphological parameters and the Bezier curve; (3) performing affine transformation according to the morphology of the hinge zone and the middle section to generate the sections at both ends of the fold; (4) generating transition sections of the 3D symbol model, based on morphing interpolation; and (5) connecting the point sets of each transition section and stitching them to obtain a 3D fold-symbol model. Case studies for different typical fold structures show that this method can eliminate excessive dependence on geological survey data in the modeling process and realize efficient, intuitive, and abstract 3D symbol modeling of fold structures based on only a few parameters. This method also applies to the 3D geological symbol modeling of faults, joints, intrusions, and other geological structures and 3D geological modeling of typical geological structures with a relatively simple spatial morphology.
A Trajectory Big Data Storage Model Incorporating Partitioning and Spatio-Temporal Multidimensional Hierarchical Organization
Authors: Yao Zhixin , Zhang Jianqin , Li Taizeng , Ding Ying
Published: December 2022

Trajectory big data is suitable for distributed storage retrieval due to its fast update speed and huge data volume, but currently there are problems such as hot data writing, storage skew, high I/O overhead and slow retrieval speed. In order to solve the above problems, this paper proposes a trajectory big data model that incorporates data partitioning and spatio-temporal multi-perspective hierarchical organization. At the spatial level, the model partitions the trajectory data based on the Hilbert curve and combines the pre-partitioning mechanism to solve the problems of hot writing and storage skewing of the distributed database HBase; at the temporal level, the model takes days as the organizational unit, finely encodes them into a minute system and then fuses the data partitioning to build spatio-temporal hybrid encoding to hierarchically organize the trajectory data and solve the problems of efficient storage and retrieval of trajectory data. The experimental results show that the model can effectively improve the storage and retrieval speed of trajectory big data under different orders of magnitude, while ensuring relatively stable writing and query speed, which can provide an efficient data model for trajectory big data mining and analysis.
Internal Iliac Artery Embolization within EVAR Procedure: Safety, Feasibility, and Outcome
Authors: Fontana Federico , Coppola Andrea , Ferrario Lucrezia , De Marchi Giuseppe , Macchi Edoardo , Zorzetto Giada , Franchin Marco , Piffaretti Gabriele , Tozzi Matteo , Carcano Giulio , Piacentino Filippo , Venturini Massimo
Published: December 2022

Background: This study is focused on Internal Iliac Artery (IIA) embolization in patients undergoing Endovascular Aneurysm Repair (EVAR). Our aims were: to establish the feasibility of the procedure; to assess the presence of endoleak (EL) and increase in the size of the sac at follow-up; to define the need for reintervention; and to evaluate mortality rate. Methods: In this retrospective single-center study, EVAR-treated patients with an embolization of IIA were chosen. Coils and vascular plug were used as embolizing agents. Results: A total of 49 participants were enrolled in the study (48 men and one woman) with a median age of 76 ± 12 years. Patients had no early EL in 87.75% of cases, 8.16% had type 1a EL, 2.04% type 1b EL, and 2.04% type 2 EL, with a comprehensive technical success of 95.91%. In the follow-up, at 1 month 72.22% remained without EL, at 6 months 70.97%, and at 1 year 81.48%. In the same period, the trend of type 1 EL was 5.56% (1 month), 3.23% (6 months), and 0% (1 year). For EL type 2: 22.22% at 1 month, 25.81% at 6 months, and 16.7% at 1 year. The overall mortality was 35.58% and the re-intervention rate was 16.33%. Conclusions: IIA embolization is a feasible and safe procedure. The presence of EL is not superior to EVAR procedures that do not involve embolization.
Automated Region of Interest Selection Improves Deep Learning-Based Segmentation of Hyper-Reflective Foci in Optical Coherence Tomography Images
Authors: Goel Sarang , Sethi Abhishek , Pfau Maximilian , Munro Monique , Chan Robison Vernon Paul , Lim Jennifer I. , Hallak Joelle , Alam Minhaj
Published: December 2022

Hyperreflective foci (HRF) have been associated with retinal disease progression and demonstrated as a negative prognostic biomarker for visual function. Automated segmentation of HRF in retinal optical coherence tomography (OCT) scans can be beneficial to identify the formation and movement of the HRF biomarker as a retinal disease progresses and can serve as the first step in understanding the nature and severity of the disease. In this paper, we propose a fully automated deep neural network based HRF segmentation model in OCT images. We enhance the model’s performance by using a patch-based strategy that increases the model’s compute on the HRF pixels. The patch-based strategy is evaluated against state of the art HRF segmentation pipelines on clinical retinal image data. Our results shows that the patch-based approach demonstrates a high precision score and intersection over union (IOU) using a ResNet34 segmentation model with Binary Cross Entropy loss function. The HRF segmentation pipeline can be used for analyzing HRF biomarkers for different retinopathies.
Is Instillational Topical Negative Pressure Wound Therapy in Peri-Prosthetic Infections of the Breast Effective? A Pilot Study
Authors: Gruener Jasmin S. , Horch Raymund E. , Geierlehner Alexander , Mueller-Seubert Wibke , Cai Aijia , Arkudas Andreas , Ludolph Ingo
Published: December 2022

Peri-prosthetic breast infections pose a risk of severe complications after breast implant surgery. The need to remove the breast implant, control the infection and perform additional surgical procedures are the consequences. Reimplantation of an alloplastic implant is only appropriate after an infection-free interval. In this retrospective cohort study, we investigated the effectiveness of negative pressure wound treatment with instillation and dwell time (NPWTi-d) on peri-prosthetic breast infections in combination with implant removal and antibiotic therapy. Twelve patients treated with NPWTi-d due to breast implant infection were included in the study. The bacterial burden was analyzed using wound swabs before and after NPWTi-d. Additionally, laboratory values were determined before NPWTi-d and immediately before wound closure. A total of 13 peri-prosthetic breast infections in 12 patients were treated using implant removal and NPWTi-d. In 76.9% (n = 10) of the cases, the patients had undergone alloplastic breast reconstruction following cancer-related mastectomy, whereas 23.1% (n = 3) of the patients had undergone breast augmentation for cosmetic reasons. The bacterial burden in the breast pocket decreased statistically significant after implant removal and NPWTi-d. No shift from Gram-positive to Gram-negative bacteria was observed. Inflammatory markers rapidly decreased following treatment. NPWTi-d had a positive impact on the healing process after peri-prosthetic breast infections, leading to a decrease in bacterial burden within the wounds and contributing to uneventful healing. Therefore, secondary reimplantation of breast prostheses might be positively influenced when compared to conventional implant removal and simple secondary closure. Further studies are required to conclusively establish the beneficial long-term effects of using NPWTi-d for the treatment of peri-prosthetic breast infections.
Amino Acids Supplementation Affects Sustainability of Productive and Meat Quality, Survivability and Nitrogen Pollution of Broiler Chickens during the Early Life
Authors: Attia Youssef A. , Al-Harthi Mohammed A. , Shafi Manal E. , Abdulsalam Nisreen M. , Nagadi Sameer A. , Wang Jinquan , Kim Woo K.
Published: December 2022

The response to amino acid (AAs) supplementation on broiler production, carcass and meat traits and nitrogen in the excreta during the early growth period was evaluated. Two experiments were performed during 1–28 d (245 birds, experiment 1) and 1–21 d of age (455 birds, experiment 2). In both experiments, the positive control (PC) diet had 22.5% crude protein (CP) and the negative control group (NC) diet had around 18% CP with the same methionine (Met) plus lysine (Lys) concentration as the PC diet. In experiment 1, the NC diet was fed to the other five groups supplemented with synthetic amino acids, such as L-arginine (Arg), L-threonine (Thr), L-valine (Val), L-isoleucine (Ile) or all these AAs, respectively. In experiment 2, the NC diet was formulated to contain 18% CP with either corn–soybean meal and animal protein or with only vegetable protein. Both NC diets were offered to the other ten groups with synthetic amino acids such as L-Arg, L-Thr, L-Val, L-tryptophan (Trp) or a combination of all these AAs plus L-isoleucine (Ile), respectively. In conclusion, broilers fed 18% CP supplemented with DL-Met plus L-Lys showed lower performance and a European production efficiency value (EPEV); Arg, Thr and Val addition improved growth, the feed conversion ratio and EPEV of the diets containing animal protein only, but broiler performance and EPEV was lower than with PC, indicating that DL-Met, L-Lys, L-Arg, L-Thr and L-Val supplementation may be limited in low-protein diets. Furthermore, a low-protein diet supplemented with amino acids did not affect the survivability of broilers up to 28 days of age.
Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, Me-col-1, in Root-Knot Nematode Meloidogyne enterolobii
Authors: Pei Ji , Feng Tuizi , Long Haibo , Chen Yuan , Pei Yueling , Sun Yanfang
Published: December 2022

Meloidogyne enterolobii, a highly pathogenic root-knot nematode species, causes serious damage to agricultural production worldwide. Collagen is an important part of the nematode epidermis, which is crucial for nematode shape maintenance, motility, and reproduction. In this study, we report that a novel collagen gene, Me-col-1, from the highly pathogenic root-knot nematode species Meloidogyne enterolobi was required for the egg formation of this pathogen. Me-col-1 encodes a protein with the size of 35 kDa, which is closely related to collagen found in other nematodes. Real-time PCR assays showed that the expression of Me-col-1 was highest in eggs and lowest in pre-parasitic second-stage juveniles (preJ2). Interestingly, knockdown of Me-col-1 did not compromise the survival rate of preJ2 but significantly reduced the egg production and consequentially caused 35.79% lower multiplication rate (Pf/Pi) compared with control. Our study provides valuable information for better understanding the function of collagen genes in the nematode life cycle, which can be used in the development of effective approaches for nematode control.
Diet-Related and Gut-Derived Metabolites and Health Outcomes: A Scoping Review
Authors: Jia Yuanxi , Yang Xuhao , Wilson Lisa M. , Mueller Noel T. , Sears Cynthia L. , Treisman Glenn J. , Robinson Karen A.
Published: December 2022

We conducted a scoping review to map available evidence about the health impact of gut microbiota-derived metabolites. We searched PubMed and Embase for studies that assessed the health impact of ten metabolites on any health condition: deoxycholate or deoxycholic acid (DCA), lithocholate or lithocholic acid (LCA), glycolithocholate or glycolithocholic acid, glycodeoxycholate or glycodeoxycholic acid, tryptamine, putrescine, d-alanine, urolithins, N-acetylmannosamine, and phenylacetylglutamine. We identified 352 eligible studies with 168,072 participants. Most (326, 92.6%) were case–control studies, followed by cohort studies (14, 4.0%), clinical trials (8, 2.3%), and cross-sectional studies (6, 1.7%). Most studies assessed the following associations: DCA on hepatobiliary disorders (64 studies, 7976 participants), colorectal cancer (19 studies, 7461 participants), and other digestive disorders (27 studies, 2463 participants); LCA on hepatobiliary disorders (34 studies, 4297 participants), colorectal cancers (14 studies, 4955 participants), and other digestive disorders (26 studies, 2117 participants); putrescine on colorectal cancers (16 studies, 94,399 participants) and cancers excluding colorectal and hepatobiliary cancers (42 studies, 4250 participants). There is a need to conduct more prospective studies, including clinical trials. Moreover, we identified metabolites and conditions for which systemic reviews are warranted to characterize the direction and magnitude of metabolite-disease associations.
New Benzofuran Oligomers from the Roots of Eupatorium heterophyllum Collected in China
Authors: Hu Yiming , Saito Yoshinori , Matsuo Yosuke , Gong Xun , Tanaka Takashi
Published: December 2022

The chemical constituents of two root samples of Eupatorium heterophyllum DC. collected in Yunnan Province, China, were investigated. Five new oligomeric benzofurans (1–5), nine new benzofuran/dihydrobenzofuran derivatives, and a new thymol analog were isolated, and their structures were determined using extensive spectroscopic techniques, such as 1D and 2D NMR spectroscopy and DFT calculations of the CD spectra. Most of the new compounds, including oligomeric benzofurans (1–5), were obtained from only one of the root samples. Furthermore, this is the first example that produces oligomeric benzofurans in this plant. These results imply that diversification of secondary metabolites in E. heterophyllum is ongoing. Plausible biosynthetic pathways for 1–5 are also proposed.
Application of Maillard Reaction Products Derived Only from Enzymatically Hydrolyzed Sesame Meal to Enhance the Flavor and Oxidative Stability of Sesame Oil
Authors: Ma Gang , He Shudong , Liu Shuyun , Zhang Zuoyong , Zhang Tao , Wang Lei , Ma Youshui , Sun Hanju
Published: December 2022

The low-temperature roasting of sesame oil has become increasingly popular because of its nutritional benefits; however, the flavor is reduced. In order to improve the quality of sesame oil without exogenous addition, sesame meal was hydrolyzed and further used to prepare Maillard reaction products (MRPs) while protease hydrolysis (PH) and glucoamylase-protease hydrolysis (GPH) were used, and their respective Maillard products (PHM and GPHM) were added in the oils for reducing sugar and total sugar content determination, free amino acid determination, and color and descriptive sensory analysis, as well as electronic nose, SPME-GC-MS, odor activity value, and oxidative stability analyses. Results showed that the MRPs could be produced using the enzymatically hydrolyzed sesame meal without exogenous addition, and the oil flavor blended with GPHM (GPHM-SO) was significantly (p < 0.05) improved with the best sensory quality. The composition of pyrazines (119.35 μg/mL), furans (13.95 μg/mL), and sulfur substances (6.25 μg/mL) contributed positively to sensory properties in GPHM-SO, and 2,5-dimethylpyrazine, 2,6-dimethylpyrazine, and 2,3-dimethylpyrazine were characterized as the key flavor compounds with odor activity values of 7.01, 14.80, and 31.38, respectively. Furthermore, the oxidative stability of the oil was significantly improved with the addition of MRPs, and the shelf life of GPHM-SO was predicted to be extended by 1.9 times more than that of the crude oil based on the accelerated oxidation fitting analysis. In general, the MRPs derived only from sesame meal can enhance the flavor and oxidative stability of sesame oil and can be applied in the oil industry.
Protective Effects of Hippophae rhamnoides L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish
Authors: Li Gang , Chu Ming , Tong Yingying , Liang Yuexin , Wang Shenghui , Ma Chengjun , Wang Zhenhua , Zhou Wenna
Published: December 2022

Hippophae rhamnoides L. is a deciduous shrub that contains many unique bioactive substances. This sea buckthorn possesses anticancer, antioxidant, anti-inflammatory, and cardiovascular protective properties. Herein, the effects of phenylpropyl compounds extracted from H. rhamnoides L. on doxorubicin (Dox)-induced cardiotoxicity were evaluated in zebrafish. Cardiac injury in zebrafish was induced using 35 μM Dox for 96 h, and 30 μM phenylpropanoid compounds were used as the protective treatment. The cardioprotective effects and mechanisms of the four phenylpropanoids were investigated using microscopy, behavioral analysis, acridine orange staining, western blotting, flow cytometry, and real-time quantitative polymerase chain reaction. The extracted phenylpropanoids could significantly relieve Dox-induced cardiac injury in zebrafish and inhibit cardiomyocyte apoptosis. The mechanisms of action were mainly related to the stability of mitochondrial biogenesis and function maintained by phenylpropanoids in zebrafish. To our knowledge, this is the first report on the protective effect of sea buckthorn against myocardial injury in zebrafish. Our findings provide support for the further research and development of sea buckthorn and its components.
Synthesis, Structural Investigations, and In Vitro/In Silico Bioactivities of Flavonoid Substituted Biguanide: A Novel Schiff Base and Its Diorganotin (IV) Complexes
Authors: Abbas Zahoor , Kumar Manoj , Tuli Hardeep Singh , Janahi Essam M. , Haque Shafiul , Harakeh Steve , Dhama Kuldeep , Aggarwal Pallvi , Varol Mehmet , Rani Anita , Sharma Shashi
Published: December 2022

Quercetin is one of the most powerful bioactive dietary flavonoids. The in vivo biological study of quercetin is extremely difficult due to its very low solubility. However, diorganotin complexes of quercetin are more useful when contrasted with quercetin due to increased solubility. In the present study, quercetin, substituted biguanide synthesized in the form of Schiff base and its di-alkyl/aryl tin (IV) complexes were obtained by condensing Schiff base with respective di-alkyl/aryl tin (IV) dichloride. Advanced analytical techniques were used for structural elucidation. The results of biological screening against Gram-positive/Gram-negative bacteria and fungi showed that these diorganotin (IV) derivatives act as potent antimicrobial agents. The in silico investigation with dihydropteroate (DHPS) disclosed a large ligand–receptor interaction and revealed a strong relationship between the natural exercises and computational molecular docking results.
Fractionation of Raw and Parboiled Rice Husks with Deep Eutectic Solvents and Characterization of the Extracted Lignins towards a Circular Economy Perspective
Authors: Allegretti Chiara , Bellinetto Emanuela , D’Arrigo Paola , Ferro Monica , Griffini Gianmarco , Rossato Letizia Anna Maria , Ruffini Eleonora , Schiavi Luca , Serra Stefano , Strini Alberto , Turri Stefano
Published: December 2022

In the present work, rice husks (RHs), which, worldwide, represent one of the most abundant agricultural wastes in terms of their quantity, have been treated and fractionated in order to allow for their complete valorization. RHs coming from the raw and parboiled rice production have been submitted at first to a hydrothermal pretreatment followed by a deep eutectic solvent fractionation, allowing for the separation of the different components by means of an environmentally friendly process. The lignins obtained from raw and parboiled RHs have been thoroughly characterized and showed similar physico-chemical characteristics, indicating that the parboiling process does not introduce obvious lignin alterations. In addition, a preliminary evaluation of the potentiality of such lignin fractions as precursors of cement water reducers has provided encouraging results. A fermentation-based optional preprocess has also been investigated. However, both raw and parboiled RHs demonstrated a poor performance as a microbiological growth substrate, even in submerged fermentation using cellulose-degrading fungi. The described methodology appears to be a promising strategy for the valorization of these important waste biomasses coming from the rice industry towards a circular economy perspective.
Isolation, Purification, and Antioxidant Activities of Polysaccharides from Choerospondias axillaris Leaves
Authors: Zhang Qiang , Lu Lianxiang , Zheng Yanfei , Qin Chengrong , Chen Yuexin , Zhou Zhongjie
Published: December 2022

The extraction, characterization and antioxidant activity of polysaccharides from Choerospondias axillaris leaves were investigated in the present study. Two purified polysaccharide fractions, CALP-1 and CALP-2, were isolated from crude Choerospondias axillaris leaf polysaccharides (CALP) by DEAE-52 cellulose chromatography and Sephadex G-100 column chromatography. The characteristics of CAL-1 and CALP-2 were determined by using High-performance Gel Permeation Chromatography (HPGPC), High-Performance Anion-Exchange Chromatography, HPAEC (HPAEC-PAD) and Fourier transform infrared spectroscopy (FTIR). CALP-1 with molecular weight of 11.20 KDa was comprised of Rhamnose, Arabinose, Galactose, Glucose, Xylose, Mannose and galacturonic acid in a molar ratio of 5.16:2.31:5.50:27.18:1.00:0.76:1.07. CAL-2 with molecular weight of 8.03 KDa consisted of Rhamnose, Arabinose, Galactose, Glucose, and galacturonic acid at a ratio of 1.38:3.63:18.84:8.28:1.45. FTIR revealed that CALP-1 and CALP-2 were acidic polysaccharides. The antioxidant activity of crude CALP, CALP-1 and CALP-2 was evaluated in vitro. The fraction CALP-2 was demonstrated to be of polysaccharide nature containing a large percentage of Galactose but no Xylose and Mannose. The antioxidant activity assays showed that CALP-1 and CALP-2 exhibited antioxidant and scavenging activities on hydroxyl and DPPH radicals in vitro. Compared with pure polysaccharide, crude CALP exhibited stronger anti-oxidant activities. These results will provide a better understanding of Choerospondias axillaris leaf polysaccharide and promote the potential applications of Choerospondias axillaris leaf polysaccharide in the pharmacological field and as a natural antioxidant.
Functional Chitosan–Calcium Carbonate Coatings for Enhancing Water and Fungal Resistance of Paper Materials
Authors: Goué Erwan Le , Ham-Pichavant Frédérique , Grelier Stéphane , Remy Jordan , Coma Véronique
Published: December 2022

The objective of this study was to increase the water resistance of paper while providing fungal resistance using a bio-based coating made from chitosan. The water resistance was improved through the surface control of roughness using modified calcium carbonate particles. The higher the quantity of particles in the film-forming solution, the higher the surface hydrophobicity of the paper. The addition of particles was found to counterbalance the chitosan hydrophilicity through the control of the coatings’ penetration in the paper bulk. As a consequence, the wetting time and liquid water resistance were enhanced. The antifungal activity of the film-forming solutions and coated paper was also investigated against the growth of Chaetomium globosum, which was selected as a model strain able to contaminate paper materials. The results reveal that the antifungal activity of chitosan was improved by a possible synergic effect with the bicarbonate ions from the mineral particles.
The Effect of Anodizing Bath Composition on the Electronic Properties of Anodic Ta-Nb Mixed Oxides
Authors: Tranchida Giada , Zaffora Andrea , Di Franco Francesco , Santamaria Monica
Published: December 2022

Anodic oxides were grown to 50 V on Ta-Nb sputtering deposited alloys, with high Nb content, in acetate ions containing an aqueous solution to study the effect of the anodizing bath composition on anodic layers’ dielectric properties. Photoelectrochemical measurements proved the presence of a photocurrent in the band gap of photon energy lower than oxides, due to optical transitions involving localized electronic states as a consequence of acetate ions incorporation. Flat band potential value estimates assessed the insulating nature of the anodic oxides grown in the acetate buffer solution. Differential capacitance measurements showed that the highest capacitance value was measured for the sample grown on Ta-66%Nb. This capacitance value was higher with respect to those estimated for pure Ta and pure Nb anodic layers and with respect to the same alloy anodized in NaOH solution, i.e., acetate-free anodizing bath.
Boosted Radiation Bystander Effect of PSMA-Targeted Gold Nanoparticles in Prostate Cancer Radiosensitization
Authors: Hara Daiki , Tao Wensi , Schmidt Ryder M. , Yang Yu-Ping , Daunert Sylvia , Dogan Nesrin , Ford John Chetley , Pollack Alan , Shi Junwei
Published: December 2022

Metal nanoparticles are effective radiosensitizers that locally enhance radiation doses in targeted cancer cells. Compared with other metal nanoparticles, gold nanoparticles (GNPs) exhibit high biocompatibility, low toxicity, and they increase secondary electron scatter. Herein, we investigated the effects of active-targeting GNPs on the radiation-induced bystander effect (RIBE) in prostate cancer cells. The impact of GNPs on the RIBE presents implications for secondary cancers or spatially fractionated radiotherapy treatments. Anti-prostate-specific membrane antigen (PSMA) antibodies were conjugated with PEGylated GNPs through EDC–NHS chemistry. The media transfer technique was performed to induce the RIBE on the non-irradiated bystander cells. This study focused on the LNCaP cell line, because it can model a wide range of stages relating to prostate cancer progression, including the transition from androgen dependence to castration resistance and bone metastasis. First, LNCaP cells were pretreated with phosphate buffered saline (PBS) or PSMA-targeted GNPs (PGNPs) for 24 h and irradiated with 160 kVp X-rays (0–8 Gy). Following that, the collected culture media were filtered (sterile 0.45 µm polyethersulfone) in order to acquire PBS- and PGNP- conditioned media (CM). Then, PBS- and PGNP-CM were transferred to the bystander cells that were loaded with/without PGNPs. MTT, γ-H2AX, clonogenic assays and reactive oxygen species assessments were performed to compare RIBE responses under different treatments. Compared with 2 Gy-PBS-CM, 8 Gy-PBS-CM demonstrated a much higher RIBE response, thus validating the dose dependence of RIBE in LNCaP cells. Compared with PBS-CM, PGNP-CM exhibited lower cell viability, higher DNA damage, and a smaller survival fraction. In the presence of PBS-CM, bystander cells loaded with PGNPs showed increased cell death compared with cells that did not have PGNPs. These results demonstrate the PGNP-boosted expression and sensitivity of RIBE in prostate cancer cells.
Thickness Effects on the Martensite Transformations and Mechanical Properties of Nanocrystalline NiTi Wires
Authors: Baigonakova Gulsharat A. , Marchenko Ekaterina S. , Kovaleva Marina A. , Chudinova Ekaterina A. , Volinsky Alex A. , Zhang Yi
Published: December 2022

This paper studied the features of the martensitic transformations and mechanical properties of 40, 60, and 90 µm thick NiTi wires with nanocrystalline B2 structures. It was established that the wires were composites and consisted of a TiNi matrix and a TiO2 + TiNi3 surface layer. Structural methods showed that the wire matrix was formed by grains of up to 20 nm in size. The method of measuring the electrical resistivity during cooling and heating revealed a two-stage nature of the martensitic transformation. Cyclic loading–unloading demonstrated that all the samples exhibited superelasticity effects and completely restored their shape when unloaded from a 4–8% relative strain at room temperature. An increase in mechanical characteristics with respect to the wire thickness was experimentally established. This was due to the change in the composition of the TiNi matrix during drawing.
Universal Character of Breaking of Wormlike Surfactant Micelles by Additives of Different Hydrophobicity
Authors: Shibaev Andrey V. , Ospennikov Alexander S. , Kuznetsova Elizaveta K. , Kuklin Alexander I. , Aliev Teimur M. , Novikov Valentin V. , Philippova Olga E.
Published: December 2022

Wormlike surfactant micelles are widely used in various applications including fracturing technology in oil industry, template synthesis of different nanoobjects, micellar copolymerization of hydrophilic and hydrophobic monomers, and so forth. Most of those applications suggest the solubilization of different additives in the micelles. The present paper is aimed at the comparative study of the effect of the solubilization of hydrophobic (n-decane and 1-phenylhexane) and hydrophilic (N-isopropylacrylamide and acrylamide) substances on the rheological properties and structure of the micelles using several complementary techniques including rheometry, small angle neutron scattering, dynamic light scattering, and diffusion ordered NMR spectroscopy. For these studies, mixed micelles of potassium oleate and n-octyltrimethylammonium bromide containing the excess of either anionic or cationic surfactants were used. It was shown that hydrophobic additives are completely solubilized inside the micelles being localized deep in the core (n-decane, 1-phenylhexane) or near the core/corona interface (1-phenylhexane). At the same time, only a small fraction of hydrophilic additives (14% of N-isopropylacrylamide and 4% of acrylamide) penetrate the micelles being localized at the corona area. Despite different localization of the additives inside the micelles, all of them induce the breaking of wormlike micelles with the formation of either ellipsoidal microemulsion droplets (in the case of hydrophobic additives) or ellipsoidal surfactant micelles (in the case of hydrophilic additives). The breaking of micelles results in the drop of viscosity of the solution up to water value. The main result of this paper consists in the observation of the fact that for all the additives under study, the dependences of the viscosity on the volume fraction of additive lie on the same master curve being shifted along the volume fraction axis by a certain factor depending on the hydrophobicity of the added species. Those data are quite useful for various applications of wormlike surfactant micelles suggesting the solubilization of different additives inside them.
Tri-Response Police, Ambulance, Mental Health Crisis Models in Reducing Involuntary Detentions of Mentally Ill People: A Systematic Review
Authors: Heffernan Julia , McDonald Ewan , Hughes Elizabeth , Gray Richard
Published: December 2022

Police, ambulance, and mental health tri-response services are a relatively new model of responding to people experiencing mental health crisis in the community, though limited evidence exists examining their efficacy. Reducing unnecessary involuntary detentions and emergency department presentations is believed to be a benefit of this model. A systematic review was performed to review the evidence base around the relationship between the police, ambulance, mental health tri-response models in reducing involuntary detentions of people experiencing mental health crisis. We searched key health databases for clinical studies and grey literature as per a previously published protocol. Two researchers completed title and abstract screening and full text screening. Our search identified 239 citations. No studies or grey literature met the inclusion criteria. We report an empty review. It is recommended that further investigation of the tri-response mental health crisis model be undertaken to determine its effectiveness and value as a health and emergency service initiative.