bluuebunny/crossref_metadata_2025 huggingface dataset:
Imaging and Image Processing Techniques for High-Resolution Visualization of Connective Tissue with MRI: Application to Fascia, Aponeurosis, and Tendon
Authors: Perera Meeghage Randika , Bydder Graeme M. , Holdsworth Samantha J. , Handsfield Geoffrey G.
Published: February 2025

Recent interest in musculoskeletal connective tissues like tendons, aponeurosis, and deep fascia has led to a greater focus on in vivo medical imaging, particularly MRI. Given the rapid T2* decay of collagenous tissues, advanced ultra-short echo time (UTE) MRI sequences have proven useful in generating high-signal images of these tissues. To further these advances, we discuss the integration of UTE with Diffusion Tensor Imaging (DTI) and explore image processing techniques to enhance the localization, labeling, and modeling of connective tissues. These techniques are especially valuable for extracting features from thin tissues that may be difficult to distinguish. We present data from lower leg scans of 30 healthy subjects using a non-Cartesian MRI sequence to acquire axial 2D images to segment skeletal muscle and connective tissue. DTI helped differentiate aponeurosis from deep fascia by analyzing muscle fiber orientations. The dual echo imaging methods yielded high-resolution images of deep fascia, where in-plane spatial resolutions were between 0.3 × 0.3 mm to 0.5 × 0.5 mm with a slice thickness of 3–5 mm. Techniques such as K-Means clustering, FFT edge detection, and region-specific scaling were most effective in enhancing images of deep fascia, aponeurosis, and tendon to enable high-fidelity modeling of these tissues.
Spatial Distribution of Illex argentinus in Different Life Stages of Mesoscale Eddies in Patagonian Waters
Authors: Zhang Lichuan , Zhang Ping , Zhang Zhong , Yu Wei
Published: February 2025

Mesoscale eddies are known to influence the abundance and distribution of oceanic cephalopods. However, little is known about these effects in the southwest Atlantic Ocean. Therefore, this study analyzed the variations in environmental conditions and the resource abundance, spatial distribution, and habitat suitability of Illex argentinus within different life stages of cyclonic (CE) and anticyclonic (AE) eddies in Patagonian waters. From a comparison of squid abundance between CEs and AEs at each life stage, it was found that I. argentinus gradually increased in abundance after eddy formation, that abundance peaked during eddy maturation and that it subsequently decreased during the eddies’ decay phase. Spatially, squid resources in AEs were primarily concentrated in the northwest and southeast peripheral regions of the eddy, while in CEs, resources were more concentrated in the outer regions, on the western side of the eddy. Environmental factor analysis revealed that sea surface temperature (SST) and temperature at 200 m depth (T200m) in both CEs and AEs reached their lowest values during the intensification and maturation phases of the eddies. Chlorophyll a (Chl-a) concentrations were significantly higher in CEs than in AEs from the formation to the maturation phase; however, during eddy decay, Chl-a concentrations were higher in AEs. According to a comparison of the suitability index (SI) for each environmental factor and the habitat suitability index (HSI) model, SISST, SIT200m, and SIChl-a in AEs increased and then decreased with eddy evolution, with optimal SI values occurring during the intensification phase. In CEs, SISST and SIT200m also increased and then decreased, with optimal SISST and SIT200m occurring during the intensification and maturation phases, respectively, with little variation in SIChl-a across the life cycle of CEs. The HSI in both types of eddies gradually increased from the formation phase, reached a peak during maturation, and significantly decreased during eddy decay. Overall, this study indicated that habitat suitability and resource abundance for I. argentinus were highest during the maturation phase of the eddies.
Sustained Nitric Oxide Release Using Hybrid Magnetic Nanoparticles for Targeted Therapy: An Investigation via Electron Paramagnetic Resonance
Authors: Salami Rawan , Lavi Ronit , Harel Yifat , Levy Esthy , Lellouche Jean Paul , Gelperina Svetlana , Persky Rachel
Published: February 2025

This research describes the development and thorough characterization of a novel, versatile, and biocompatible hybrid nanocarrier of the NO-releasing agent NOC-18, with a specific focus on optimizing the purification process. In this study, we focused on the sustained release of NO using biocompatible and diagnostic hybrid magnetic nanoparticles (hMNPs) containing cerium-doped maghemite (CM) NPs, embedded within human serum albumin (HSA) protein. A comprehensive study was conducted using electron paramagnetic resonance (EPR) alongside the Griess assay to evaluate NO release from the chosen NO donor, NOC-18, and to assess the limitations of the molecule under various reaction conditions, identifying the optimal conditions for binding NOC-18 with minimal NO loss. Two types of particles were designed: In-hMNPs, where NOC-18 is encapsulated within the particles, and Out-hMNPs, where NOC-18 is attached onto the surface. Our results demonstrated that In-hMNPs provided a sustained and prolonged release of NO (half-life, 50 h) compared to the rapid release for the Out-hMNPs, likely due to the strong bonds formed with cerium, which helped to stabilize the NO molecules. These results represent a promising approach to designing a dual-function agent that combines contrast properties for tumor MRI with the possibility of increasing the permeability of tumor vasculature. The employment of this dual-function agent in combination with nanotherapeutics could improve the latter’s efficacy by facilitating their access to the tumor.
Multiomic Analysis Provided Insights into the Responses of Carbon Sources by Wood-Rotting Fungi Daldinia carpinicola
Authors: Yang Peng , Ma Xingchi , Zhang Yu , Sun Yanan , Yu Hao , Han Jiandong , Ma Meng , Wan Luzhang , Cheng Fansheng
Published: February 2025

Daldinia carpinicola is a newly identified species of wood-rotting fungi, with substantial aspects of its biology and ecological function yet to be clarified. A Nanopore third-generation sequencer was employed for de novo genome assembly to examine the genetic characteristics. The genome consisted of 35.93 Mb in 46 contigs with a scaffold N50 of 4.384 Mb. Glycoside hydrolases and activities enzymes accounted for a large proportion of the 522 identified carbohydrate-active enzymes (CAZymes), suggesting a strong wood degradation ability. Phylogenetic and comparative analysis revealed a close evolutionary relationship between D. carpinicola and D. bambusicola. D. carpinicola and Hypoxylon fragiforme exhibited significant collinear inter-species genome alignment. Based on transcriptome and metabolomic analyses, D. carpinicola showed a greater ability to utilize sucrose over sawdust as a carbon source, enhancing its growth by activating glycolysis/gluconeogenesis and the citrate cycle. However, compared with sucrose, sawdust as a carbon source activated D. carpinicola amino acid biosynthesis and the production of various secondary metabolites, including diterpenoid, indole alkaloid, folate, porphyrin, and biotin metabolism. The study establishes a theoretical basis for research and applications in biological processes, demonstrating a strategy to modulate the production of secondary metabolites by altering its carbon sources in D. carpinicola.
Longitudinal Study on Clinical Predictors for Allergic Bronchopulmonary Aspergillosis in Children and Young People with Cystic Fibrosis Highlights the Impact of Infection with Aspergillus and Pseudomonas and Ivacaftor Treatment
Authors: Chesshyre Emily L. D. , Enderby Beth , Shore Angela C. , Warren Fiona C. , Warris Adilia
Published: February 2025

Allergic bronchopulmonary aspergillosis (ABPA) is a well-known complication in children and young people with cystic fibrosis (CF) and without treatment causes structural lung damage. We performed a longitudinal observational study to identify clinical risk factors for ABPA in a cohort of children and young people with CF aged 8 to 17 years at baseline. Anonymised annual review UK CF Registry data from 2009 to 2019 for patients aged 8–17 years in 2009 were collected, with lung transplant recipients excluded. Baseline characteristics are presented for the whole group and cross-sectional comparisons made according to the presence of ABPA or not in 2009. Longitudinal analysis from 2009 to 2019 was completed on the group without ABPA in 2009 to identify predictors for the subsequent development of ABPA using a complementary log–log regression model. In 2009, there were 1612 patients, of which 1420 were ABPA-negative and 192 ABPA-positive. Aspergillus colonisation (p = 0.01) and IV antibiotic use (p < 0.0001) were associated with having ABPA in 2009. Longitudinal analysis of the group without ABPA in 2009 identified male gender, younger age, lower lung function, Pseudomonas aeruginosa infection, and Aspergillus colonisation to be significantly associated with the development of ABPA (p < 0.0001). Ivacaftor was significantly associated with reduced ABPA (OR 0.46, p = 0.01) but not lumacaftor/ivacaftor (OR 0.64, p = 0.28). Chronic oral macrolide use was significantly associated with increased risk of development of ABPA (OR 1.30, p < 0.0001). This study shows that lower lung function, Aspergillus colonisation, and Pseudomonas aeruginosa infection in children with CF were associated with the development of ABPA, highlighting the need for enhanced surveillance in these patients. This is the first study to show a protective association of ivacaftor and ABPA.
The Effect of SMEs’ Digitalization on Supply Chain Financing Performance: Based on the Resource Orchestration Theory
Authors: Lu Qiang , Qin Wangzhe , Yan Rui , Zhang Songbo , Ma Lianyao
Published: February 2025

This study aims to use resource orchestration theory (ROT) to investigate how small and medium-sized enterprise (SME) digitalization affects supply chain financing performance (SCFP) by examining the mediation effect of ambidextrous innovations. It also investigates the moderating role of environmental competitiveness on the relationship between SME digitalization and SCFP, and ambidextrous innovations, respectively. A valid representative sample for this study was drawn from 343 Chinese SMEs through a questionnaire survey. Multiple regression analysis was used to test the hypotheses. The study revealed a positive impact of SMEs’ digitalization on SCFP. Meanwhile, exploitative innovation, not exploratory innovation, plays a partial mediating role between SMEs’ digitalization and SCFP. Environmental competitiveness strengthens the positive relationship between SMEs’ digitalization and SCFP. Moreover, environmental competitiveness strengthens the positive relationship between SMEs’ digitalization and exploratory innovation. This study is one of the first to identify digitalization as a key antecedent of SCFP and the mediating role of ambidextrous innovations on the relationship between SMEs’ digitalization and SCFP. Also, this is the first study to explore the moderating role of environmental competitiveness in the context of digitalization.
Notch and Hedgehog Signaling Unveiled: Crosstalk, Roles, and Breakthroughs in Cancer Stem Cell Research
Authors: Iluta Sabina , Nistor Madalina , Buruiana Sanda , Dima Delia
Published: February 2025

The development of therapies that target cancer stem cells (CSCs) and bulk tumors is both crucial and urgent. Several signaling pathways, like Notch and Hedgehog (Hh), have been strongly associated with CSC stemness maintenance and metastasis. However, the extensive crosstalk present between these two signaling networks complicates the development of long-term therapies that also minimize adverse effects on healthy tissues and are not overcome by therapy resistance from CSCs. The present work aims to overview the roles of Notch and Hh in cancer outburst and the intersection of the two pathways with one another, as well as with other networks, such as Wnt/β-catenin, TGF, and JAK/STAT3, and to explore the shaping of the tumor microenvironment (TME) with specific influence on CSC development and maintenance.
Dynamical Properties for a Unified Class of One-Dimensional Discrete Maps
Authors: Conejero J. Alberto , Lizama Carlos , Quijada David
Published: February 2025

Currently, despite advances in the analysis of dynamical systems, there are still doubts about the transition between both stable and chaotic behaviors. In this research, we will explain the transition of a system that develops between two dynamic systems that have already been studied: the classical logistic model and a new chaotic system. This research addresses the study of the transition of both the system and its behaviors using computational techniques, where cobweb diagrams, time series, bifurcation diagrams, and even a graphical visualization for the maximum Lyapunov exponent will be visualized. Using a graphical and numerical methodology, bifurcation points were identified that revealed the transition of behaviors at different points. This resulted in a deep understanding of the dynamics of the system, thus highlighting the importance of incorporating computational analysis in dynamic systems, which greatly contributes to the efficient modeling of natural phenomena.
Morphometric Analysis of Neocortical and Infratentorial Structures: Genetic and Environmental Insights from a Twin Neuroanatomical Study
Authors: Alijanpourotaghsara Amirreza , Vessal Arsalan , Alijanpour Amirmasoud , Strelnikov David , Piroska Marton , Persely Aliz , Jokkel Zsofia , Szalontai Laszlo , Forgo Bianka , Kozak Lajos Rudolf , Bekesy-Szabo Adam , Maurovich-Horvat Pal , Tarnoki David Laszlo , Tarnoki Adam Domonkos
Published: February 2025

Background and Objective: Brain morphometry is shaped by a complex interplay of genetic and environmental factors, including physiological and neuropsychiatric conditions. These influences can vary across distinct brain regions, yet the precise contributions of genetics and environment to regional variation in healthy brains remain poorly understood. This study examines the heritability of specific brain structures to provide deeper insights into their development. Materials and Methods: We studied 118 healthy adult twins from the Hungarian Twin Registry using T1-weighted magnetic resonance imaging (T1W MRI) and the volBrain pipeline for structural measurements. Results: In all regions, monozygotic (MZ) twins showed a higher resemblance than dizygotic (DZ) twins in total brainstem and cerebellar volumes, with significant heritability (A: 90.5–92.6%) and minimal unique environmental effects (E: <1%). For supratentorial regions, regarding the total gray matter volume, all regions exhibited high heritability (A: 74.5–92.4%) and minimal environmental influence (E: <1.5%). In average cortical thickness analysis, the frontal lobe, temporal lobe, and pre-central gyrus were influenced by shared and unique environmental factors (C: 63–66.5%; E: 33.4–37%), whereas genetics were more prominent in the parietal lobe, occipital lobe, and post-central gyrus (A: 67.7–85%; E: 15–32.3%). Conclusions: Genetics strongly influence cortical gray matter volume in supratentorial regions (both total and regional), as well as the total brainstem volume and the total and cortical gray matter volumes of the cerebellum in infratentorial regions. This genetic influence extends to the average cortical thickness of the parietal lobe, post-central gyrus, and occipital lobe, while the frontal lobe, temporal lobe, and pre-central gyrus are more affected by environmental factors. These findings emphasize the importance of understanding region-specific genetic and environmental contributions to brain structure, which could guide personalized therapeutic and preventive strategies for neurological conditions.
Protective and Therapeutic Effects of Orlistat in Combination with Elettaria cardamomum “Cardamom” Extract on Learning, Memory, Anxiety, and Neuroinflammation in Obese Mice
Authors: AL-Dalaeen Anfal , Batarseh Nour , Abdelhadi Nadine N. , Atawneh Sally , AbuKashef Reem , Al-Yasari Ali Mosa Ra-shid
Published: February 2025

Introduction and Objective: Obesity has increased worldwide, and existing anti-obesity medications have treatment limitations that diminish their overall benefits. This study aimed to investigate the effects of orlistat in combination with Elettaria cardamomum “Cardamom” (CAR) extract on working memory, recognition memory, anxiety, and inflammation within hippocampal tissue. Methods: Mice were categorized into two groups: a control group (CD) and a cafeteria diet (CAF) group induced with obesity (CAF) for 10 weeks. The groups were then subdivided into a CAF group treated with orlistat (CAF-ORL), a CAF group treated with orlistat and Elettaria cardamomum (CAF-ORL-CARD), and a group that continued on the CAF. The CAF-ORL group received orlistat at a dosage of 10 mg/kg/day for four weeks, while the CAF-ORL-CARD group received 10 mg/kg/day of orlistat and 500 mg/kg of CAR extract via oral gavage. In the 14th week, various assessments were conducted, including the novel object recognition (NOR) test, Y maze test, marble-burying test (MBT), open-field test, and TNF-α levels in the hippocampus. Result: TNF-α levels in the hippocampal tissue of the CAF group were elevated compared to the CD group (p < 0.01), whereas the CAF-ORL group exhibited reduced TNF-α levels compared to the CAF group (p < 0.01). Moreover, TNF-α levels in the CAF-ORL-CARD group were significantly lower than in the CAF-ORL group (p < 0.01). The recognition index was notably higher in the CAF-ORL group compared to the CAF group (p < 0.01) and higher in the CAF-ORL-CARD group compared to the CAF-ORL group (p < 0.01). However, there were no changes in the open-field test and Y maze test (p > 0.05). Conclusions: Orlistat combined with CAR has positive effects on neuroinflammation and memory, suggesting that this combination may offer potential therapeutic benefits for cognitive impairments and hippocampal dysfunction associated with obesity.
Visit-to-Visit Systolic Blood Pressure Variability and Risk of Ischemic and Hemorrhagic Stroke
Authors: Sandu Oana Elena , Bogdan Carina , Apostol Adrian , Daniluc Larissa Madalina-Alexandra , Schuldesz Amanda Claudia , Simu Mihaela Adriana
Published: February 2025

Background and Objectives: Systolic blood pressure (SBP) variability has been increasingly associated with cardiovascular outcomes, including stroke. This study aimed to evaluate the association between visit-to-visit SBP variability and the risk of ischemic and hemorrhagic stroke. Materials and Methods: A prospective cohort study was conducted on a set of 208 hypertensive patients over a period of three years, from August 2021 to September 2024, at the County Emergency Hospital “Pius Brinzeu”, Timișoara. Patients included in the study were stroke-free. SBP variability was quantified as the standard deviation of SBP measurements obtained quarterly. Results: This study demonstrated that systolic blood pressure (SBP) variability serves as a robust predictor of stroke incidence, underscoring its important role in cerebrovascular risk. The study cohort had an average age of 65.3 ± 9.1 years, with 53.4% males and 46.6% females. Patients in the highest SBP variability group had a 1.21-fold increased risk (21%, p = 0.031) of ischemic stroke and a 1.73-fold increased risk (73%, p = 0.005) of hemorrhagic stroke compared to those in the lowest variability group, revealing that higher SBP variability is strongly associated with an increased risk of both ischemic and hemorrhagic strokes, with the relationship being particularly pronounced for hemorrhagic stroke. Patients exhibiting greater fluctuations in SBP experienced significantly earlier stroke events and reduced stroke-free survival. Moreover, mortality rates were notably higher among individuals with very high SBP variability, indicating its profound impact on long-term outcomes. Conclusions: Visit-to-visit SBP variability is a significant and independent predictor of both ischemic and hemorrhagic stroke, emphasizing the clinical importance of monitoring and managing blood pressure stability. Further research should explore interventions to mitigate SBP variability and its impact on cerebrovascular outcomes.
Magnetic Pulse Powder Compaction
Authors: Mironovs Viktors , Nikitina Jekaterina , Kolbe Matthias , Boiko Irina , Usherenko Yulia
Published: February 2025

Powder metallurgy (PM) offers several advantages over conventional melt metallurgy, including improved homogeneity, fine grain size, and pseudo-alloying capabilities. Transitioning from conventional methods to PM can result in significant enhancements in material properties and production efficiency by eliminating unnecessary process steps. Dynamic compaction techniques, such as impulse and explosive compaction, aim to achieve higher powder density without requiring sintering, further improving PM efficiency. Among these techniques, magnetic pulse compaction (MPC) has gained notable interest due to its unique process mechanics and distinct advantages. MPC utilizes the rapid discharge of energy stored in capacitors to generate a pulsed electromagnetic field, which accelerates a tool to compress the powder. This high-speed process is particularly well-suited for compacting complex geometries and finds extensive application in industries such as powder metallurgy, welding, die forging, and advanced material manufacturing. This paper provides an overview of recent advancements and applications of MPC technology, highlighting its capabilities and potential for broader integration into modern manufacturing processes.
Effects of Bi and In Addition on Microstructure and Properties of Sn-0.7Cu Solder
Authors: Lv Xiaochun , Pan Zhen , Liu Yang , Zhang Chenghao , Wang Zhiyuan , Sun Fenglian
Published: February 2025

With the widespread application of lead-free solder, solder represented by the SAC series has been widely used. However, with the miniaturization and multifunctionalization of electronic devices, the distance between solder joints is becoming increasingly smaller, and the problem of electrochemical migration caused by Ag in the SAC series is gradually emerging. Therefore, it becomes imperative to develop a solder that has a melting point, mechanical properties, and other properties similar to those of the SAC series but does not contain silver. In this study, 1–3% of Bi and 1–3% of In were added to the Sn-0.7Cu solder to investigate the effects of these elements on the melting characteristics, microstructure, mechanical properties, wettability, and electrical conductivity of the Sn-0.7Cu solder. Due to the addition of Bi and In elements, the melting point of the Sn-0.7Cu solder was lowered, the shear strength was improved, and the solderability was enhanced, but the electrical conductivity was reduced. This article obtained the mechanism of the influence of Bi and In elements on the properties of the Sn-0.7Cu solder through experiments and theoretical analysis, providing a certain degree of theoretical support for the development of silver-free solder.
Metabolomics in Pathogenic Pathways and Targeted Therapies for Diabetic Neuropathy: A Comprehensive Review
Authors: Bala Cornelia , Rusu Adriana , Ciobanu Dana Mihaela , Roman Gabriela , Crăciun Anca Elena
Published: February 2025

Introduction and objective: This literature review aims to provide an overview of the progress in metabolomic assessment in animal and cell models and in humans with diabetic neuropathy (DN). Methods: Metabolomics has emerged as an important approach for investigating, identifying, and describing biomarkers related to DN. None has yet been validated for use in clinical practice. Results: DN induced significant alterations in energy metabolism and carbohydrates, lipids, amino acids, peptides, and proteins. Several treatments for DN, evaluated using metabolomics, were proved to have promising results. Conclusions: The ideal metabolite or set of metabolites that could be used as biomarkers should identify patients with diabetes prone to develop DN or those prone to progress to severe forms of sensory loss, associated with risk of ulcerations and amputation. Another potential use of a metabolite might be as an indicator of treatment response in clinical trials using agents with potential disease-modifying properties.
Exploring the Antineoplastic Properties of the Lebanese Jania rubens Against Colorectal Cancer
Authors: Rifi Mariam , Radwan Zeina , Sari-Chmayssem Nouha , Kassir Rayan , Fajloun Ziad , Abdel Rahman Abir , El-Sabban Marwan , Prévostel Corinne , Dassouki Zeina , Mawlawi Hiba
Published: February 2025

Background/Objective: Colon cancer poses a significant health burden, with current treatments often associated with severe side effects and limited effectiveness for some patients. Natural products are gaining interest as adjuvant therapies, potentially reducing side effects and improving responses to conventional treatments. We previously highlighted the potent antineoplastic effects of organic extracts derived from the Lebanese red algae Jania rubens. This study, investigated the anticancer activities of polysaccharide, protein, and lipid extracts from J. rubens, which may serve as adjuvant therapies to enhance conventional treatments. Methods: we employed colorimetric assays, wound healing assays, and cell cycle analysis to evaluate the anticancer activities of the extracts. The polysaccharide extract was characterized for sulfate content and structure using barium chloride-gelatin and FT-IR methods. Results: All J. rubens extracts exhibited significant anticancer effects, with the polysaccharide extract showing particularly strong cytotoxicity, apoptosis induction, and antiproliferative and anti-migratory activities. Conclusion: These findings confirm that J. rubens is a source of bioactive compounds with anticancer potential. Further investigations are needed to elucidate the molecular pathways targeted by J. rubens extracts in cancer cells.
Identification and Characterization of Two Se6OMTs from Stephania epigaea Offer Novel Insights into the Biosynthetic Pathway of Cepharanthine
Authors: Gan Jingyi , Shi Wenlong , Li Qishuang , Li Xinyi , Zhao Xingyu , Tang Junhao , Ma Ying , Wang Jian , Gong Shukun , Ma Xiaohui , Guo Juan
Published: February 2025

Background/Objectives: Stephania epigaea is a plant from the Menispermaceae family. Its root is an important traditional folk medicine, which is called Diburong in China. Diburong is rich in benzylisoquinoline alkaloids (BIAs), including cepharanthine, which has been demonstrated to exhibit significant anti-inflammatory, antiviral, antineoplastic, and anti-SARS-CoV-2 activities, as well as raising leukocytes. Cepharanthine is composed of (R)- and (S)-1-benzylisoquinoline alkaloid (1-BIA). (S)-norcoclaurine-6-O-methyltransferase (6OMT) is a rate-limiting enzyme in BIA biosynthesis. However, its role in the cepharanthine biosynthetic pathway, particularly with the (R) stereoisomer substrate, remains largely unexplored. This study aimed to identify Se6OMTs involved in the cepharanthine biosynthetic pathway and elucidate the O-methyltransferases (OMTs) responsible for the production of (R)- and (S)-stereoisomer BIAs. Methods: In this study, three OMTs were cloned from S. epigaea and functionally characterized using nine 1-BIAs of (R)- and (S)-configurations as substrates. Results: Two O-methyltransferases, Se6OMT1 and Se6OMT3, showed efficient catalytic activity at the C6 position of both (R)- and (S)-norcoclaurine. Furthermore, Se6OMT3 demonstrated high catalytic activity at the C7 and C4′ positions of other (R)- and (S)-configuration 1-BIAs, which resulted in the generation of multiple products. Conclusions: This study focused on 6OMT enzymes in S. epigaea, identifying Se6OMTs involved in the cepharanthine biosynthetic pathway, determining the OMTs involved in the production of (R)- and (S)-stereoisomer BIAs. This research provides valuable insights into the substrate promiscuity of Se6OMTs on (R)- and (S)-configured 1-BIAs in S. epigaea and highlights the genetic components necessary for the metabolic engineering and synthetic biology approaches to cepharanthine production.
Design and Fabrication of a Piezoelectric Bimorph Microphone with High Reliability and Dynamic Range Based on Al0.8Sc0.2N
Authors: Yan Ruixiang , Ji Yucheng , Liu Anyuan , Wang Lei , Zhang Songsong
Published: February 2025

With the development of technology, MEMS microphones, which are small-sized and highly uniform, have been applied extensively. To improve their reliability in extreme environment and overcome the constraints of traditional microphones, this article presents a piezoelectric bimorph MEMS microphone using Al0.8Sc0.2N. In the article, the high robustness of piezoelectric microphones and the reasons for choosing Al0.8Sc0.2N as piezoelectric materials are described. The sensitivity of an Al0.8Sc0.2N-based piezoelectric bimorph compared with the traditional structure are revealed through FEA. Subsequently, a lumped element microphone model is constructed and all noise sources are evaluated comprehensively. The difference in output noise caused by different structures is calculated. The designed piezoelectric microphone, which comprises eight triangular cantilever beams, was fabricated on a chip with an area of 900 μm × 900 μm. The sensitivity of the designed microphone achieves 1.68 mV/Pa, with a noise floor of −110 dBA and SNR of 54.5 dB. The acoustic overload point of the microphone stands at 147 dB SPL, and following the impact test, the survival rate was 100%. Compared to traditional MEMS microphones, the microphone achieves a dynamic range of 107.5 dB.
Knockin’ on Cell’s Door: Influenza A Virus Adsorption and Its Pharmacological Inhibition
Authors: Gorshkov Andrey , Varyushina Elena
Published: February 2025

Influenza A virus (IAV) is a widespread human respiratory pathogen that contributes significantly to morbidity and mortality worldwide. The adsorption of the virus into the cell surface is the earliest stage of its replication cycle. The key role of N-linked sialic acids (SIAs) as receptors for binding to IAV’s hemagglutinin (HA) has long been acknowledged. The molecular specificity of this interaction is a key factor in host range, pathogenicity, and transmissibility of various IAV subtypes. Along with this, a number of recent studies have introduced significant complexity into the picture of IAV adsorption and revealed a multitude of new molecules on host cell surfaces to serve as receptors and/or co-receptors for IAV attachment. For successful internalization of the adsorbed virus, downstream signal transduction is necessary to activate effector endocytosis mechanisms. In recent years, our understanding of the sophistication and variability of signal transduction pathways in the virus attachment site has significantly expanded, with the help of research techniques like fluorescence imaging of individual viruses in real-time, dominant-negative mutants, siRNA knockdowns, protein kinase selective inhibitors, phosphoproteome profiling, and others. These approaches deepen our knowledge of the molecules involved in the early stages of the IAV life cycle and also serve as the basis for the development of new effective antiviral drugs. In our review, we analyze recent publications on the mechanisms of IAV adsorption, newly discovered receptors for virus attachment, and signal transmission in the site of the adsorbed virion. Besides this, we consider new data on the development of selective inhibitors as antiviral drugs aimed at both viral and cellular factors of IAV adsorption.
Isolation and Bioassay of Linear Veraguamides from a Marine Cyanobacterium (Okeania sp.)
Authors: Parker Stacy-Ann J. , Hough Andrea , Wright Thomas , Lax Neil , Faruk Asef , Fofie Christian K. , Simcik Rebekah D. , Cavanaugh Jane E. , Kolber Benedict J. , Tidgewell Kevin J.
Published: February 2025

Marine cyanobacteria have gained momentum in recent years as a source of novel bioactive small molecules. This paper describes the structure elucidation and pharmacological evaluation of two new (veraguamide O (1) and veraguamide P (2)) and one known (veraguamide C (3)) analogs isolated from a cyanobacterial collection made in the Las Perlas Archipelago of Panama. We hypothesized that these compounds would be cytotoxic in cancer cell lines. The compounds were screened against HEK-293, estrogen receptor positive (MCF-7), and triple-negative breast cancer (MDA-MB-231) cells as well as against a broad panel of membrane-bound receptors. The planar structures were determined based on NMR and MS data along with a comparison to previously isolated veraguamide analogs. Phylogenetic analysis of the collection suggests it to be an Okeania sp., a similar species to the cyanobacterium reported to produce other veraguamides. Veraguamide O shows no cytotoxicity (greater than 100 μM) against ER-positive cells (MCF-7) with 13 μM IC50 against MDA-MB-231 TNBC cells. Interestingly, these compounds show affinity for the sigma2/TMEM-97 receptor, making them potential leads for the development of non-toxic sigma 2 targeting ligands.
Safety, Sensory Quality and Nutritional Value of Hybrid Meat Products Made from Turkey Meat and Red Beans Preserved with a Bioprotective Culture
Authors: Karwowska Małgorzata , Skwarek Patrycja , Solska Elżbieta , Nowaczyk Agata , Goławski Andrzej , Wojtaś Przemysław , Stasiak Dariusz M.
Published: February 2025

The current study assessed the quality and safety of hybrid meat products made from turkey meat and red beans with the addition of SAFEPRO® B-LC-20 protective cultures. The tested materials were hybrid products produced with turkey thigh muscles and red beans in 100:0, 60:40, 50:50 and 40:60 ratios. During a 15-day storage period, research was carried out on the physicochemical and microbiological properties, antioxidant capacity, fatty acid profile and sensory characteristics. The results showed that the count of Enterobacteriaceae in hybrid meat products did not differ significantly depending on the formulation. The addition of red beans in a hybrid meat product formulation significantly increased the antioxidant activity of the products compared to a sample made of 100% meat. The samples with red beans were characterized by significantly lower values of n-6/n-3, UFA/SFA and PUFA/SFA compared to samples produced with turkey thigh muscles and red beans in a ratio of 100:0. In summary, the formulation combining turkey meat and beans in a ratio of 60:40 is recommended as optimal, enabling the creation of a safe hybrid meat product with properties similar to those of a full-meat product.
Exploratory Ultrasound Analysis of the Diaphragm and Respiratory Capacity in Women with Primary Dysmenorrhea: A Cross-Sectional Observational Study
Authors: del Prado-Álvarez Rebeca , García-Arrabé María , González-de-la-Flor Ángel , de la Plaza San Frutos Marta , Almazán-Polo Jaime , Estrada-Barranco Cecilia
Published: February 2025

Primary dysmenorrhea (PD) is a common gynecological condition characterized by menstrual pain without underlying pelvic pathology. It has been linked to functional and structural changes in the core musculature, but limited evidence exists regarding its association with diaphragmatic and respiratory mechanics. This study aimed to elaborate on these potential associations by assessing the diaphragmatic structure and respiratory function in women with PD compared to healthy controls, utilizing ultrasound imaging, spirometry and respiratory pressure measurements. Methods: An observational, cross-sectional study was conducted with 44 female participants (22 with PD and 22 healthy controls). Diaphragmatic structure was evaluated through ultrasound, measuring the intercostal distance, diaphragmatic thickness, and diaphragmatic excursion at rest and during maximum voluntary contraction. Spirometric assessments included forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), and the FVC/FEV1 ratio, along with measurements of maximum inspiratory pressure (MIP) and maximum expiratory pressure (MEP). Group differences were analyzed using Student’s t-test and effect sizes were reported with Cohen’s d. Results: No significant differences were observed between the groups in diaphragmatic thickness, diaphragmatic excursion, or global respiratory capacity (p > 0.05). However, women with PD presented a significant reduction in the left intercostal distance both at rest (p = 0.035, d = 0.56) and during contraction (p = 0.039, d = 0.54). No other significant group differences were detected. Conclusions: While primary dysmenorrhea does not appear to affect overall diaphragmatic function or respiratory capacity, it is associated with subtle localized changes in the left intercostal dynamics. These findings suggest a potential compensatory mechanical adaptation rather than global respiratory dysfunction. Further longitudinal studies with larger sample sizes are needed to explore the clinical significance of these findings.
Nurses’ Perspectives on the Sleep Quality of Hospitalized Patients in Al Ahsa, Saudi Arabia
Authors: El Arab Rabie Adel , Alzghoul Husam , Abu-Mahfouz Mohammad S. , Aldarwish Zainab , Abbadi Mariam , Ghashi Eman , Alsaffar Ghasaq , Alasmkh Wujd , Seweid Mohamed Mahmoud
Published: February 2025

Background: Sleep quality is crucial for patient recovery and well-being, yet hospitalized patients often suffer from poor sleep due to environmental disruptions, clinical routines, and psychosocial stressors. While these challenges are well-documented, qualitative insights into nurses’ perspectives—essential frontline providers shaping the sleep environment—are scarce, especially within rapidly evolving healthcare systems like Saudi Arabia’s. This study explores nurses’ perceptions of factors influencing patient sleep quality in a private hospital in Al Ahsa, Saudi Arabia, and identifies strategies for improvement. Methods: We conducted a qualitative, cross-sectional study using semi-structured interviews with 14 registered nurses from diverse nationalities, specialties (Obstetrics/Gynecology, Medical-Surgical, Pediatrics, Intensive Care, Orthopedics, Bariatrics), and experience levels. Interviews were conducted in Arabic or English, audio-recorded, transcribed, and thematically analyzed using ATLAS.ti software. Roy’s Adaptation Model guided the examination of environmental, patient-specific, and systemic factors affecting sleep. Findings: Four primary themes emerged: (1). Environmental Factors: noise from alarms, equipment, family presence, and late-night activities, along with abrupt lighting changes, consistently disrupted sleep. (2). Patient-Specific Factors: pain, emotional distress, cultural expectations, and family involvement influenced sleep experiences. (3). Systemic and Contextual Factors: language barriers, infrastructural disparities between private and governmental hospitals, and limited resources can impeded effective sleep-promoting strategies. (4). Role of Health Technology: nurses recognized the potential of innovations like smart lighting and wearable monitors to enhance sleep quality but faced challenges in implementation due to knowledge gaps and limited familiarity. Roy’s Adaptation Model highlighted how effective adaptation through physiological and cognitive–emotional pathways, as observed by nurses, was facilitated or hindered by these factors. Conclusions: Enhancing in-hospital sleep quality requires a holistic, culturally sensitive approach that integrates environmental modifications, patient-centered care, and systemic improvements. Strategic investments in staff communication training, infrastructural upgrades, language support services, and the adoption of health technologies can promote adaptive responses and optimize patient rest. By leveraging theory-driven insights and context-specific strategies, healthcare systems—particularly those undergoing rapid development—can better support nurses in fostering restorative sleep environments as a fundamental component of patient-centered care, thereby enhancing patient recovery, satisfaction, and overall well-being.
Adaptive Construction of Freeform Surface to Integrable Ray Mapping Using Implicit Fixed-Point Iteration
Authors: Chen Jiahua , Zhou Yangui , He Hexiang , Li Yongyao
Published: February 2025

Constructing a freeform surface that accurately satisfies both integrable condition and Snell’s law under a given invariant source–target map is challenging for freeform design. Here, we propose a fixed-point iteration method to address this problem. This process involves solving a set of balanced gradient equations in the form of fixed-point iterations that are derived from equivalent integrability conditions and Snell’s law. By using the convergence theorem of fixed-point iteration, a unique solution for the balanced gradient equations exists, which is determined by the natural geometric properties of the freeform surface and is independent of the mapping. The gradient operators on the left-hand side of the equations are converted into a differential matrix form via a finite difference scheme. In one iteration, differential operations are forward-performed on the right-hand side of the equations, and the system of linear equations is solved on the left-hand side of the equation. The constructed freeform surfaces work well in both the paraxial and nonparaxial regions, and convergence in the nonparaxial region is faster than that in the paraxial region. The robustness and high efficiency of the proposed method are demonstrated with several design examples.
Preparation and Hg0 Removal Performance of MIL-101(Cr)-Derived Carbon Matrix Composites
Authors: Nie Haotian , Li Zikuo , Zhang Xikai , Wen Jinchao , Feng Youxiang , Yu Yue , Jia Li
Published: February 2025

The temperature at which pollutants are treated varies across different industrial processes. To address the high cost of raw materials for MOFs and the low efficiency of Hg0 removal in low-temperature environments, a series of MIL-101(Cr)-derived carbon matrix composite materials were prepared by combining MIL-101(Cr) with biomass and multiple metals. These materials were synthesized through a sol-gel method followed by carbonization. This study investigates the effects of composite ratios and adsorption temperatures on Hg0 removal, utilizing XRD, BET, and other characterization techniques to elucidate the mercury-removal mechanism of the PDC-MIL composite materials. The results indicate that MIL101(Cr) significantly influences the formation of the gel skeleton. When the composite ratio of MIL-101(Cr) to biomass is 1:1, the material exhibits an optimal pore structure, leading to high Hg0 removal efficiency over a wide temperature range. The removal of Hg0 by these composite materials involves both physical adsorption and chemisorption. Low temperatures favor physical adsorption, while high temperatures promote chemisorption. The sol-gel composite method facilitates cross-linking polymerization between MOFs and SiO2, enabling better pore structure connectivity with biomass and MOFs, thereby optimizing the poor pore structure observed after pyrolysis. Consequently, the improved pore structure enhances physical adsorption at low temperatures, mitigates desorption at high temperatures, and increases the contact probability of Hg0 with active sites within the pores, significantly improving the mercury-removal ability of the material across a broad temperature range.
Evaluation of the Pharmacological Activities of a Xylan from Corn Cobs
Authors: Nobrega Rayssa Lourenna Trigueiro , Silva Viana Rony Lucas , Silva Marianna Barros , Duarte Martins Matta Luciana , Souza Giulianna Paiva Viana Andrade , Oliveira Rocha Hugo Alexandre , Fagundes Melo-Silveira Raniere
Published: February 2025

Xylans, polysaccharides abundantly derived from agricultural byproducts, have shown potential pharmacological properties, making them a subject of increasing research interest. This study aimed to expand the understanding of xylans’ pharmacological properties and relate them to their composition. A method combining ultrasound and alkaline media for xylan extraction from corn cobs (ERX) was used, resulting in a significant increase in final yield compared to other methodologies. The physicochemical characterization of ERX was carried out, and its antioxidant, cytotoxic, anticoagulant, and immunomodulatory properties were evaluated. ERX demonstrated significant antioxidant activity with metal-chelating properties and induced apoptosis in HeLa tumor cells (p < 0.0001). It also reduced nitric oxide (NO) production by activated macrophages and extended the blood coagulation time, as assessed by the APTT assay (p < 0.0001). Further fractionation of ERX using various organic solvents resulted in multiple xylan subfractions. Among them, the ethanol-derived subfraction E1.4 exhibited remarkable pharmacological activities, including metal-chelation, cytotoxicity against HeLa cells via apoptosis, reduced NO production (p < 0.0001), and prolonged coagulation times (p < 0.0001). E1.4 is heteroxylan with a molecular weight of approximately 100 kDa. These findings suggest that corn cobs could be a promising source of pharmacologically significant molecules, particularly the heteroxylan E1.4. Future studies should focus on the structural characterization of this xylan to understand the relationship between structure and biological activity and explore the therapeutic potential of E1.4 in vivo models.
Radial Basis Function Neural Network and Response Surface Methodology-Based Optimization of Glue Breakage in Waste Drilling Fluids
Authors: Li Yan , Xu Ziqiang , Xi Jiaxin , Zhao Hanlei , Fan Zheng
Published: February 2025

This paper aims to solve the problems of difficult breakage and low water yield of wastewater-based drilling fluids. The effects of breakage agent dosage, reaction temperature, stirring speed, and pumping time on the water yield of wastewater-based drilling fluids were analyzed through one-way experiments, and these parameters were optimized by Response Surface Methodology (RSM). A Radial Basis Function Neural Network (RBF) model was also introduced to compare its performance with that of RSM in predicting optimal process conditions. To further improve the accuracy of the model, a combination of Genetic Algorithm (GA) and RBF was used for optimization. The results show that both RSM and RBF models can predict the water yield of wastewater-based drilling fluids with high accuracy, in which the coefficient of determination of the RSM model is 0.9939, which is better than that of the RBF model (0.9778). The optimal operating conditions are determined through numerical optimization: the amount of glue breaker added is 3.97 kg/m3, the reaction temperature is 51 °C, the stirring speed is 419 rpm, and the maximum water yield is 62.37%, which is the best-predicted water yield. The GA-RBF coupled model performed better in terms of root mean square error (RMSE), coefficient of determination (R2 = 0.998), and mean absolute error (MAE), and the t-test verified that there was no significant difference between the predicted and actual values. The maximum value of the water yield of the waste mud could reach 61.97% when the gum breaker was added at 3.83 kg/m3, the reaction temperature was 53 °C, and the stirring speed was 424 rpm, which provided an accurate and reliable theoretical basis and technical support for the harmless treatment of deep and complex drilling fluids.