bluuebunny/crossref_metadata_2025 huggingface dataset:
‘Is that okay, teacher?’ The camera as a tool to challenge power relations in a participatory action research classroom
Authors: Hemy Avy Dwight , Meshulam Assaf
Published: September 2020

Conducting a participatory action research (PAR) in schools is challenged by traditional asymmetrical power relations between adult teacher-researcher and young student-participants inherent in the school setting. In this article, we present PowerView, a new method that may reduce power hierarchy in the research classroom. Based on postcolonial theory, feminist theories, and critical visual studies, we implemented the idea of ‘reversal-of-the-gaze’ by asking the student-participants in our PAR program to turn their cameras at the instructor-researcher and capture images that represent their point of view of him. Enabling the students’ to gaze back at the instructor-researcher/serial observer with their cameras disrupted the hierarchical power paradigm in the research classroom and created a more equal space. The article will introduce the methodological stages of PowerView and present findings that demonstrate the potential of the method to change power relations between the researcher and students and challenge the power structure at the research classroom.
The continuing significance of social structure in liquid modernity
Authors: Caldwell Marylouise , Henry Paul Conrad
Published: September 2020

Bauman’s liquid modernity has been influential among consumer culture theorists in recent times. A key element of this thinking is that old social structures have broken down, consumers being less encumbered by their structural origins and freer to pursue more diverse lifestyles. The ensuing three commentaries critically examine the claim that old social structures have become less relevant. Caldwell and Henry detail some of the critiques of Bauman and point out that consumers’ management of liquidity versus solidity can be usefully understood using a Bourdieusian lens. Their arguments are informed by contemporary literature focusing on social class, family life cycle and mythical role preferences. Thompson and Kumar argue that inconspicuous consumption masks social class-based differences in the distribution of resources that act to maintain structural inequalities. Parsons and Cappellini explore how conditions of liquidity mean that some people can accrue value much more easily than others. These authors argue that the enabling capacities that prevail under conditions of liquidity (e.g. flexibility, idiosyncratic taste) are not new but rather have long been known to act as enablers of success among advantaged classes.
Commercialization of Creative Videos in China in the Digital Platform Age
Authors: Lin Zhongxuan
Published: September 2020

With a special focus on the commercialization of creative videos, this article explores the research question of how digital platforms’ affordances simultaneously allow and constrain video producers’ commercialization activities in the platform era. This study adopts a case study design that focuses on the Chinese digital video producer Zheng Yun, founder of Zheng Yun Studio, using ethnographic participant observations and in-depth interviews. It explains how creative producers such as Zheng Yun struggle to survive in the context of intensified platformization and how they benefit from the digital platforms by employing various commercialization mechanisms, including the Revenue Sharing Program (RSP), Embedded Product Placement (EPP), Franchise Chains, Agent-commission, and Crowd-funding. This research also demonstrates the asymmetrical power relationships between platforms and video producers, which prompt us to rethink the political nature of platforms and the diversified nature of platformization in the digital platform age.
Experimental verification of the virtual isotropic material concept for the last-ply-failure of U-notched quasi-isotropic E-glass/epoxy composite laminates under tension-shear loading
Authors: Torabi Ali Reza , Pirhadi Ebrahim
Published: September 2020

Fracture investigation of U-notched E-glass/epoxy laminated composite specimens with various notch root radii is performed under mixed mode I/II loading conditions both experimentally and theoretically. Rectangular E-glass/epoxy composite laminates with two numbers of ply (8-ply and 16-ply) and the quasi-isotropic [0/90/+45/–45]s lay-up configuration are fabricated for conducting the fracture tests. To measure the damage initiation angles (DIAs) and the last-ply-failure (LPF) loads of the fabricated composite samples containing horizontal and inclined U-notches, as the main aim of this study, the specimens are loaded under tension by the universal testing machine. The experimental LPF loads are theoretically predicted with the aid of a novel concept, called the Virtual Isotropic Material Concept (VIMC). The proposed VIMC is linked to the two well-known stress-based fracture criteria, namely the maximum tangential stress (MTS) and the mean stress (MS) criteria, in the context of the linear elastic notch fracture mechanics (LENFM). It is proved that the two combined criteria, namely the VIMC-MTS and VIMC-MS criteria, can predict well the LPF loads of the U-notched laminated composite specimens tested under mixed mode I/II loading conditions. One of the important merits of these new criteria is that the predictions of the LPF loads of the U-notched composite specimens are performed without complicated and time-wasting ply-by-ply damage analyses.
Deoxycholic Acid Upregulates the Reprogramming Factors KFL4 and OCT4 Through the IL-6/STAT3 Pathway in Esophageal Adenocarcinoma Cells
Authors: Chen Mei , Ye AXiaojun , Wei Jingxi , Wang Ruihua , Poon Karen
Published: September 2020

Cancer stem cells, a special subgroup of cancer cells, have self-renewal capabilities and multidirectional potential, which may be reprogrammed from the dedifferentiation of cancer cells, contributing to the failure of clinical treatments. Esophageal adenocarcinoma grows in an inflammatory environment stimulated by deoxycholic acid, an important component of gastroesophageal reflux content, contributing to the transformation of esophageal squamous epithelium to the precancerous lesions of esophageal adenocarcinoma, that is, Barrett esophagus. In the present study, deoxycholic acid was used to investigate whether it could induce the expression of reprogramming factors Krüppel-like factor, OCT4, and Nanog; the transformation to cancer stem cells in esophageal adenocarcinoma; and the involvement of the interleukin-6/signal transduction and activation of transcription 3 inflammatory signaling pathway. OE33 cells were treated with deoxycholic acid (250 μM) for 0 hour, 3 hours, 6 hours, and 12 hours before evaluating the messenger RNA expression of Krüppel-like factor, OCT4, Nanog, interleukin-6, and Bcl-xL by reverse transcription-quantitative polymerase chain reaction. Interleukin-6 protein was detected by enzyme linked immunosorbent assay, while signal transduction and activation of transcription 3, phosphorylated signal transduction and activation of transcription 3, Krüppel-like factor, and OCT4 were detected by Western blot. Signal transduction and activation of transcription 3 small interfering RNA and human recombinant interleukin-6 were used to treat OE33 cells and to detect their effects on Krüppel-like factor, OCT4, Nanog, CD44, hypoxia-inducible factor 1-α, and Bcl-xL expression. Results showed that deoxycholic acid promotes the expression of reprogramming factors Krüppel-like factor and OCT4, which are regulated by the interleukin-6/signal transduction and activation of transcription 3 signaling pathway. Deoxycholic acid has a malignancy-inducing effect on the transformation of esophageal adenocarcinoma stem cells, improving the antiapoptotic ability of tumors, and increasing the malignancy of esophageal adenocarcinoma. Deactivating the regulatory signaling pathway of interleukin-6/signal transduction and activation of transcription 3 and neutralizing deoxycholic acid may be novel targets for improving the clinical efficacy of esophageal adenocarcinoma therapy.
Left Gastric Pseudoaneurysm in a Case of Chronic Pancreatitis: A Case Report With Review of Literature
Authors: Nagarajan Krishnan , Sunilkumar Devaraj , Ramakrishnaiah Vishnu Prasad Nelamangala , Amuthabarathi Mohan
Published: September 2020

Visceral artery pseudoaneurysms are common complications of pancreatitis that carry a high mortality rate. Early detection and treatment of the pseudoaneurysms should be done as they carry high mortality following rupture. The splenic artery is the commonest artery involved followed by gastroduodenal and pancreaticoduodenal arteries. Gastric artery pseudoaneurysms are less common with less than 50 cases reported in the literature till now. We describe a case of gastric artery pseudoaneurysm secondary to chronic pancreatitis in a patient who presented with intermittent GI bleeding and also describe the role of endovascular management in the treatment of the same.
Dilutional local anaesthetic techniques in oncoplastic breast surgery and potential benefits during the COVID-19 pandemic and beyond
Authors: Batt Jeremy , Cook Nicola , Nadeem Muhammad , Sahu Ajay
Published: September 2020

Introduction

COVID-19 has changed the approach to operating on breast cancer for the benefit of patients, staff and the general population. One approach involves the switch from operating under general to local anaesthetic. We assess whether diluational local anaesthetic is as effective as the current standard approach.

Methods

Postoperative pain was recorded in prospective, consecutive patients undergoing wide local excision under dilutional local anaesthetic (concentration < 1mg/ml). Pain scores were documented at 0, 30 and 60 minutes and compared to a control group consisting of combined general with local anaesthetic.

Results

Pain significantly increased in the control group during the postoperative recovery. This was not seen in the dilutional local anaesthetic group that was non-inferior to the standard approach at 0, 30 and 60 minutes.

Conclusion

Dilutional local anaesthetic provides a safe and effective alternative approach to operating on breast cancer patients whilst avoiding risky general anaesthetic in a COVID-19 pandemic environment.
Sociology and the Sustainable Development Goals: Or, Do We Really Have a Role in Changing the World?
Authors: Billson Janet Mancini
Published: September 2020

The Millennium Development Goals of 2000 to 2015 have been replaced by the Sustainable Development Goals (SDGs) of 2015 to 2030. How can these global aspirations guide sociologists in bringing our knowledge, skills, and insights to achieve justice and equality of opportunity? This essay relates each of the SDGs to our fundamental expertise.
Survival in/at the Intersections: A Preface for Three Papers on the Matter of Things
Authors: Alexander Bryant Keith
Published: September 2020

These papers were independently submitted to the Ethnography Division and the Disability Issues Caucus for the National Communication Association 2019 Annual Convention. The panel was entitled: “Survival in/at the Intersections: Contributed Papers on Race, Sexuality and Disability.” The substantive content of the papers in general bleed the disciplinary borders between self and community located in particular and shifting cultural contexts. These three essays take into consideration the ways in which place and space, positions and positionalities, as well as populations and politics always impact our shifting sense of self; and how desire and distain are held in tensive relations. Each essay sustains and promotes an activism of the self—through auto/ethnographic means that consistently promote critical qualitative scholarship in/as/for social justice, to change culture and to sustain community, especially as a response to how others construct us (as them).
Vector-borne diseases in pregnancy
Authors: O’Kelly Brendan , Lambert John S.
Published: September 2020

Vector-borne infections cause a significant proportion of world-wide morbidity and mortality and many are increasing in incidence. This is due to a combination of factors, primarily environmental change, encroachment of human habitats from urban to peri-urban areas and rural to previously uninhabited areas, persistence of poverty, malnutrition and resource limitation in geographical areas where these diseases are endemic. Pregnant women represent the single largest ‘at risk’ group, due to immune-modulation and a unique physiological state. Many of these diseases have not benefitted from the same level of drug development as other infectious and medical domains, a factor attributing to the ‘neglected tropical disease’ title many vector-borne diseases hold. Pregnancy compounds this issue as data for safety and efficacy for many drugs is practically non-existent, precluding exposure in pregnancy to many first-line therapeutic agents for ‘fear of the unknown’ or overstated adverse pregnancy-foetal outcomes. In this review, major vector-borne diseases, their impact on pregnancy outcomes, current treatment, vaccination and short-comings of current medical practice for pregnant women will be discussed.
Knowledge, attitude and practice of lifestyle modification recommended for hypertension management and the associated factors among adult hypertensive patients in Harar, Eastern Ethiopia
Authors: Bogale Saron , Mishore Kirubel Minsamo , Tola Assefa , Mekuria Abraham Nigussie , Ayele Yohanes
Published: September 2020

Introduction:

Hypertension is an overwhelming global challenge. Appropriate lifestyle modifications are the cornerstone for the prevention and control of hypertension. In this regard, lack of knowledge and poor attitude toward lifestyle modification have been a major setback.

Objective:

To assess knowledge, attitude and practice of lifestyle modification recommended for hypertension management and the associated factors among adult hypertensive patients in Harar, Eastern Ethiopia.

Methods:

Hospital-based cross-sectional study was conducted among 274 hypertensive patients in Hiwot Fana Specialized University Hospital, from 1 March to 30 May 2019. The pre-tested structured questionnaire was used, and the data were collected through an interview. The data were analyzed using SPSS version 20. A multivariate logistic regression model was fitted to determine independent predictors of knowledge and practice of lifestyle modifications among hypertensive patients. Adjusted odds ratio (AOR) at 95% confidence interval (CI) was used for predicting the independent effect of each variable on the outcome variables.

Results:

From the total participants, 200 (73.0%) of participants had good knowledge, 182 (66.4%) had favorable attitude and 136 (49.6%) had good practice on lifestyle modification recommended for hypertension management. Regarding factors associated with lifestyle modification, being in age range of 46–64 years (AOR: 4.08, 95% CI: 1.14–14.56); having formal education (AOR: 3.93, 95% CI: 1.27–12.23); being government employee (AOR: 8.06, 95% CI: 1.40–46.32) and being housewives (AOR: 5.10, 95% CI: 1.26–20.79) were factors significantly associated with good knowledge of lifestyle modification, However, favorable attitude was found to be the only factor associated with good practice of lifestyle modification (AOR: 9.20, 95% CI: 2.60–32.24).

Conclusion:

In the current study, knowledge and attitude toward lifestyle modification recommended for hypertension management was fairly good but practice level was poor. Concerted strategies are required to increase the knowledge, attitude and practice of the lifestyle modification measures in this population group.
Demystifying Social Bots: On the Intelligence of Automated Social Media Actors
Authors: Assenmacher Dennis , Clever Lena , Frischlich Lena , Quandt Thorsten , Trautmann Heike , Grimme Christian
Published: September 2020

Recently, social bots, (semi-) automatized accounts in social media, gained global attention in the context of public opinion manipulation. Dystopian scenarios like the malicious amplification of topics, the spreading of disinformation, and the manipulation of elections through “opinion machines” created headlines around the globe. As a consequence, much research effort has been put into the classification and detection of social bots. Yet, it is still unclear how easy an average online media user can purchase social bots, which platforms they target, where they originate from, and how sophisticated these bots are. This work provides a much needed new perspective on these questions. By providing insights into the markets of social bots in the clearnet and darknet as well as an exhaustive analysis of freely available software tools for automation during the last decade, we shed light on the availability and capabilities of automated profiles in social media platforms. Our results confirm the increasing importance of social bot technology but also uncover an as yet unknown discrepancy of theoretical and practically achieved artificial intelligence in social bots: while literature reports on a high degree of intelligence for chat bots and assumes the same for social bots, the observed degree of intelligence in social bot implementations is limited. In fact, the overwhelming majority of available services and software are of supportive nature and merely provide modules of automation instead of fully fledged “intelligent” social bots.
Relationships Between Narrow Personality Traits and Self-Regulated Learning Strategies: Exploring the Role of Mindfulness, Contingent Self-Esteem, and Self-Control
Authors: Opelt Felicitas , Schwinger Malte
Published: September 2020

Based on Boekaerts’ dual-processing self-regulation model, our study seeks to examine to what extent narrow personality traits are associated with different aspects of self-regulated learning. We approach this research question by examining the relationships of the narrow personality traits mindfulness, contingent self-esteem (domain academic competence), and self-control with various self-regulated learning strategies, emotion regulation strategies, and affective well-being during learning in a sample of N = 588 university students. Path analysis revealed the selected narrow personality traits to be significantly related to a variety of the investigated self-regulated learning variables, with more relationships to self-control and the contingent self-esteem domain academic competence than to mindfulness. Our study overall contributes to the understanding of how the selected narrow personality traits of students are associated with various aspects of their self-regulated learning behavior. Practical implications for promoting self-regulated learning via training (self-control) and counseling services (contingent self-esteem) are discussed.
Beyond experiments: Embedding outcomes in climate governance
Authors: Sengers Frans , Turnheim Bruno , Berkhout Frans
Published: September 2020

Concerted action on climate change will require a continuing stream of social and technical innovations whose development and transmission will be influenced by public policies. New ways of doing things frequently emerge in innovative small-scale initiatives – ‘experiments’ – across sectors of economic and social life. These experiments are actionable expressions of novel governance and socio-technical arrangements. Mobilising and generalising the outputs of these experiments could lead to deep reductions in greenhouse gas emissions over the long-term. It is often assumed that the groundswell of socio-technical and governance experiments will ‘scale-up’ to systemic change. But the mechanisms for these wider, transformative impacts of experiments have not been fully conceptualised and explained. This paper proposes a conceptual framework for the mobilisation, generalisation and embedding of the outputs and outcomes of climate governance experiments. We describe and illustrate four ‘embedding mechanisms’ – (1) replication-proliferation; (2) expansion-consolidation; (3) challenging-reframing; and (4) circulation-anchoring – for entwined governance and socio-technical experiments. Through these mechanisms knowledge, capabilities, norms and networks developed by experiments become mobile and generic, and come to be embedded in reconfigured socio-technical and governance systems.
Sexual(ities that) progress: Introduction
Authors: Browne Kath , Lim Jason , Hall Joseph , McGlynn Nick
Published: September 2020

This piece introduces the papers for the Sexual(ities that) Progress special issue. It arises out of two sessions at the 2017 American Association of Geographers Annual Conference, where scholars critically interrogated assumptions of progress and the ideals and models that follow from understanding certain spaces and places as ‘leading the way’ in terms of sexual and gender inclusions. In this paper, we outline some of the key debates and how papers in this special issue address discourses of sexual(ities that) progress and, in particular, the importance of decolonial and postcolonial critiques in such debates. We conclude by noting omissions, the timeliness of the papers and the ongoing need for spatial lenses in exploring the power relationships that reconstitute sexual and gendered lives, cultures, politics and embodiments.
Development of the 2017 national seismic hazard maps of Indonesia
Authors: Irsyam Masyhur , Cummins Phil R , Asrurifak M , Faizal Lutfi , Natawidjaja Danny Hilman , Widiyantoro Sri , Meilano Irwan , Triyoso Wahyu , Rudiyanto Ariska , Hidayati Sri , Ridwan M , Hanifa Nuraini Rahma , Syahbana Arifan Jaya
Published: September 2020

Indonesia is one of the most seismically active countries in the world, and its large, vulnerable population makes reliable seismic hazard assessment an urgent priority. In 2016, the Indonesian Ministry of Public Works and Housing established a team of earthquake scientists and engineers tasked with improving the input data available for revising the national seismic hazard map. They compiled results of recent active fault studies using geological, geophysical, and geodetic observations, as well as a new comprehensive earthquake catalog including hypocenters relocated in a three-dimensional velocity model. Seismic hazard analysis was undertaken using recently developed ground motion prediction equations (GMPEs), and logic trees for the inclusion of epistemic uncertainty associated with different choices for GMPEs and earthquake recurrence models. The new seismic hazard maps establish the importance of active faults and intraslab seismicity, as well as the subduction megathrust, in determining the level of seismic hazard, especially in onshore, populated areas. The new Indonesian hazard maps will be used to update national standards for design of earthquake-resilient buildings and infrastructure.
Early BCR-ABL1 kinetics are predictive of subsequent achievement of treatment-free remission in chronic myeloid leukemia
Authors: Shanmuganathan Naranie , Pagani Ilaria S. , Ross David M. , Park Sahee , Yong Agnes S. M. , Braley Jodi A. , Altamura Haley K. , Hiwase Devendra K. , Yeung David T. , Kim Dong-Wook , Branford Susan , Hughes Timothy P.
Published: September 2020

With treatment-free remission (TFR) rapidly becoming the ultimate goal of therapy in chronic myeloid leukemia (CML), there is a need to develop strategies to maximize sustained TFR by improving our understanding of its key determinants. Chronic-phase CML patients attempting TFR were evaluated to identify the impact of multiple variables on the probability of sustained TFR. Early molecular response dynamics were included as a predictive variable, assessed by calculating the patient-specific halving time of BCR-ABL1 after commencing tyrosine kinase inhibitor (TKI) therapy. Overall, 115 patients attempted TFR and had ≥12 months of follow-up. The probability of sustained TFR, defined as remaining in major molecular response off TKI therapy for 12 months, was 55%. The time taken for the BCR-ABL1 value to halve was the strongest independent predictor of sustained TFR: 80% in patients with a halving time of <9.35 days (first quartile) compared with only 4% if the halving time was >21.85 days (last quartile) (P < .001). The e14a2 BCR-ABL1 transcript type and duration of TKI exposure before attempting TFR were also independent predictors of sustained TFR. However, the BCR-ABL1 value measured at 3 months of TKI was not an independent predictor of sustained TFR. A more rapid initial BCR-ABL1 decline after commencing TKI also correlated with an increased likelihood of achieving TFR eligibility. The association between sustained TFR and the time taken for BCR-ABL1 to halve after commencing TKI was validated using an independent dataset. These data support the critical importance of the initial kinetics of BCR-ABL1 decline for long-term outcomes.
Results of a phase 2 trial of an oral CXCR4 antagonist, mavorixafor, for treatment of WHIM syndrome
Authors: Dale David C. , Firkin Frank , Bolyard Audrey Anna , Kelley Merideth , Makaryan Vahagn , Gorelick Kenneth J. , Ebrahim Tarek , Garg Varun , Tang Weihua , Jiang Honghua , Skerlj Renato , Beaussant Cohen Sarah
Published: September 2020

Warts, hypogammaglobulinemia, infections, and myelokathexis (WHIM) syndrome is a rare primary immunodeficiency caused by gain-of-function mutations in the CXCR4 gene. We report the safety, tolerability, pharmacokinetics, pharmacodynamics, and preliminary efficacy of mavorixafor from a phase 2 open-label dose-escalation and extension study in 8 adult patients with genetically confirmed WHIM syndrome. Mavorixafor is an oral small molecule selective antagonist of the CXCR4 receptor that increases mobilization and trafficking of white blood cells from the bone marrow. Patients received escalating doses of mavorixafor, up to 400 mg once daily. Five patients continued on the extension study for up to 28.6 months. Mavorixafor was well tolerated with no treatment-related serious adverse events. At a median follow-up of 16.5 months, we observed dose-dependent increases in absolute neutrophil count (ANC) and absolute lymphocyte count (ALC). At doses ≥300 mg/d, ANC was maintained at >500 cells per microliter for a median of 12.6 hours, and ALC was maintained at >1000 cells per microliter for up to 16.9 hours. Continued follow-up on the extension study resulted in a yearly infection rate that decreased from 4.63 events (95% confidence interval, 3.3-6.3) in the 12 months prior to the trial to 2.27 events (95% confidence interval, 1.4-3.5) for patients on effective doses. We observed an average 75% reduction in the number of cutaneous warts. This study demonstrates that mavorixafor, 400 mg once daily, mobilizes neutrophil and lymphocytes in adult patients with WHIM syndrome and provides preliminary evidence of clinical benefit for patients on long-term therapy. The trial was registered at www.clinicaltrials.gov as #NCT03005327.
Double unrelated umbilical cord blood vs HLA-haploidentical bone marrow transplantation: the BMT CTN 1101 trial
Authors: Fuchs Ephraim J. , O’Donnell Paul V. , Eapen Mary , Logan Brent , Antin Joseph H. , Dawson Peter , Devine Steven , Horowitz Mary M. , Horwitz Mitchell E. , Karanes Chatchada , Leifer Eric , Magenau John M. , McGuirk Joseph P. , Morris Lawrence E. , Rezvani Andrew R. , Jones Richard J. , Brunstein Claudio G.
Published: September 2020

Results of 2 parallel phase 2 trials of transplantation of unrelated umbilical cord blood (UCB) or bone marrow (BM) from HLA-haploidentical relatives provided equipoise for direct comparison of these donor sources. Between June 2012 and June 2018, 368 patients aged 18 to 70 years with chemotherapy-sensitive lymphoma or acute leukemia in remission were randomly assigned to undergo UCB (n = 186) or haploidentical (n = 182) transplant. Reduced-intensity conditioning comprised total-body irradiation with cyclophosphamide and fludarabine for both donor types. Graft-versus-host disease prophylaxis for UCB transplantation was cyclosporine and mycophenolate mofetil (MMF) and for haploidentical transplantation, posttransplant cyclophosphamide, tacrolimus, and MMF. The primary end point was 2-year progression-free survival (PFS). Treatment groups had similar age, sex, self-reported ethnic origin, performance status, disease, and disease status at randomization. Two-year PFS was 35% (95% confidence interval [CI], 28% to 42%) compared with 41% (95% CI, 34% to 48%) after UCB and haploidentical transplants, respectively (P = .41). Prespecified analysis of secondary end points recorded higher 2-year nonrelapse mortality after UCB, 18% (95% CI, 13% to 24%), compared with haploidentical transplantation, 11% (95% CI, 6% to 16%), P = .04. This led to lower 2-year overall survival (OS) after UCB compared with haploidentical transplantation, 46% (95% CI, 38-53) and 57% (95% CI 49% to 64%), respectively (P = .04). The trial did not demonstrate a statistically significant difference in the primary end point, 2-year PFS, between the donor sources. Although both donor sources extend access to reduced-intensity transplantation, analyses of secondary end points, including OS, favor haploidentical BM donors. This trial was registered at www.clinicaltrials.gov as #NCT01597778.
Potential Diagnostic Systems for Coronavirus Detection: a Critical Review
Authors: Ekrami Elena , Pouresmaieli Mahdi , Barati Fatemeh , Asghari Sahar , Ziarani Farzad Ramezani , Shariati Parvin , Mamoudifard Matin
Published: September 2020

Currently there are no effective anti-viral drugs for SARS-CoV-2, so the primary line of defense is to detect infected cases as soon as possible. The high rate of contagion for this virus and the highly nonspecific symptoms of the disease (Coronovirus disease 2019, (Covid-19)) that it causes, such as respiratory symptoms, cough, dyspnea, fever, and viral pneumonia, require the urgent establishment of precise and fast diagnostic tests to verify suspected cases, screen patients, and conduct virus surveillance. Nowadays, several virus detection methods are available for viral diseases, which act on specific properties of each virus or virus family, therefore, further investigations and trials are needed to find a highly efficient and accurate detection method to detect and prevent the outcomes of the disease. Hence, there is an urgent need for more and precise studies in this field. In this review, we discussed the properties of a new generation of coronaviruses (SARS-CoV-2) following routine virus detection methods and proposed new strategies and the use of potential samples for SARS-CoV-2 detection.

Graphical Abstract
Identification and characterization of the bacteriocin Carocin S3 from the multiple bacteriocin producing strain of Pectobacterium carotovorum subsp. carotovorum
Authors: Wang Jyun-Wei , Derilo Reymund C. , Lagitnay Ruchi Briam James S. , Wu Huang-Pin , Chen Kai-In , Chuang Duen-Yau
Published: September 2020

Background

Pectobacterium carotovorum subsp. carotovorum belongs to the Enterobacteriaceae family, which causes soft-rot disease in numerous plants worldwide resulting in significant economic losses. Results from our previous studies showed that the strain H-rif-8-6 produces low-molecular-weight bacteriocin (LMWB) Carocin S1. Interestingly, TH22–10, the caroS1K:Tn5 insertional mutant in H-rif-8-6, loses Carocin S1 producing ability, but still produces other LMWBs which the indicator strain SP33 can detect. The SP33 is one of the many strains that are sensitive toward the cytotoxic effects of Carocin S3K, but not Carocin S1. The result revealed that H-rif-8-6 is a multiple-bacteriocin producing strain.

Results

In this study, a 4.1-kb DNA fragment was isolated from the chromosomal DNA of Pcc strain, H-rif-8-6, by a DNA probe using the caroS1K gene as the template. DNA sequencing and analysis by GenBank revealed two complete open reading frames (ORFs), designated ORF1 and ORF2, which were identified within the sequence fragment. ORF1 and ORF2, similar to the identified carocin S2 genes, encode the killer (Carocin S3K) and the immunity (Carocin S3I) proteins, respectively, which were homologous to the colicin E3 gene. Carocin S3K and Carocin S3I were expressed, isolated, and purified in Escherichia coli BL21 after subcloning of the expression plasmid pGS3KI or pGSK3I. SDS-PAGE analysis showed that the relative masses of Carocin S3K and Carocin S3I were 95.6 kDa and 10.2 kDa, respectively. The results reveal that Carocin S3K has higher antimicrobial and specific antimicrobial activities for Pcc along with a nuclease activity than Carocin S3I. However, Carocin S3I inhibits the activity of Carocin S3K. Interestingly, a high concentration of Carocin S3I protein is also a DNA nuclease, and Carocin S3K also inhibits its activity.

Conclusion

This study showed that another type of bacteriocin was found in Pectobacterium carotovorum. This new type of bacteriocin, Carocin S3, has the killer protein, Carocin S3K, and the immunity protein, Carocin S3I.
De novo assembly of transcriptome and genome-wide identification reveal GA3 stress-responsive WRKY transcription factors involved in fiber formation in jute (Corchorus capsularis)
Authors: Zhang Lilan , Wan Xuebei , Xu Yi , Niyitanga Sylvain , Qi Jianmin , Zhang Liwu
Published: September 2020

Background

WRKY is a group of transcription factors (TFs) that play a vital role in plant growth, development, and stress tolerance. To date, none of jute WRKY (CcWRKY) genes have been identified, even if jute (Corchorus capsularis) is one of the most important natural fiber crops in the world. Little information about the WRKY genes in jute is far from sufficient to understand the molecular mechanism of bast fiber biosynthesis.

Results

A total of 244,489,479 clean reads were generated using Illumina paired-end sequencing. De novo assembly yielded 90,982 unigenes with an average length of 714 bp. By sequence similarity searching for known proteins, 48,896 (53.74%) unigenes were annotated. To mine the CcWRKY TFs and identify their potential function, the search for CcWRKYs against the transcriptome data of jute was performed, and a total of 43 CcWRKYs were identified in this study. The gene structure, phylogeny, conserved domain and three-dimensional structure of protein were analyzed by bioinformatics tools of GSDS2.0, MEGA7.0, DNAMAN5.0, WebLogo 3 and SWISS-MODEL respectively. Phylogenetic analysis showed that 43 CcWRKYs were divided into three groups: I, II and III, containing 9, 28, and 6 members respectively, according to the WRKY conserved domain features and the evolution analysis with Arabidopsis thaliana. Gene structure analysis indicated that the number of exons of these CcWRKYs varied from 3 to 11. Among the 43 CcWRKYs, 10, 2, 2, and 14 genes showed higher expression in leaves, stem sticks, stem barks, and roots at the vigorous vegetative growth stage, respectively. Moreover, the expression of 21 of 43 CcWRKYs was regulated significantly with secondary cell wall biosynthesis genes using FPKM and RT-qPCR by GA3 stress to a typical GA3 sensitive dwarf germplasm in comparison to an elite cultivar in jute. The Cis-element analysis showed that promoters of these 21 CcWRKYs had 1 to 4 cis-elements involved in gibberellin-responsiveness, suggesting that they might regulate the development of bast fiber in response to GA3 stress.

Conclusions

A total of 43 CcWRKYs were identified in jute for the first time. Analysis of phylogenetic relationship and gene structure revealed that these CcWRKYs might have a functional diversity. Expression analysis showed 21 TFs as GA3 stress responsive genes. The identification of these CcWRKYs and the characterization of their expression pattern will provide a basis for future clarification of their functions in bast fiber development in jute.
Feasibility of containing shigellosis in Hubei Province, China: a modelling study
Authors: Rui Jia , Chen Qi , Chen Qiuping , Hu Qingqing , Hannah Mikah Ngwanguong , Zhao Zeyu , Wang Yao , Liu Xingchun , Lei Zhao , Yu Shanshan , Chiang Yi-Chen , Zhao Benhua , Su Yanhua , Zhao Bin , Chen Tianmu
Published: September 2020

Background

The transmission features and the feasibility of containing shigellosis remain unclear among a population-based study in China.

Methods

A population–based Susceptible – Exposed – Infectious / Asymptomatic – Recovered (SEIAR) model was built including decreasing the infectious period (DIP) or isolation of shigellosis cases. We analyzed the distribution of the reported shigellosis cases in Hubei Province, China from January 2005 to December 2017, and divided the time series into several stages according to the heterogeneity of reported incidence during the period. In each stage, an epidemic season was selected for the modelling and assessing the effectiveness of DIP and case isolation.

Results

A total of 130,770 shigellosis cases were reported in Hubei Province. The median ofReffwas 1.13 (range: 0.86–1.21), 1.10 (range: 0.91–1.13), 1.09 (range: 0.92–1.92), and 1.03 (range: 0.94–1.22) in 2005–2006 season, 2010–2011 season, 2013–2014 season, and 2016–2017 season, respectively. The reported incidence decreased significantly (trendχ2 = 8260.41,P <  0.001) among four stages. The incidence of shigellosis decreased sharply when DIP implemented in three scenarios (γ = 0.1, 0.1429, 0.3333) and when proportion of case isolation increased.

Conclusions

Year heterogeneity of reported shigellosis incidence exists in Hubei Province. It is feasible to contain the transmission by implementing DIP and case isolation.
Using Q-methodology to understand the perspectives and practical experiences of dermatologists about treatment difficulties of cutaneous leishmaniasis
Authors: Qurtas Dindar S. , Shabila Nazar Pauls
Published: September 2020

Background

During the outbreak of cutaneous leishmaniasis in the Kurdistan Region of Iraq that started in 2015, the course of the disease and the treatment were not consistent with the available literature. Physicians, particularly dermatologists, faced challenges with treating the cutaneous leishmaniasis lesions with high rates of treatment failure and resistance to treatment. We used Q-methodology to understand the range and diversities of opinions and the practical experiences of dermatologists about the treatment difficulties of cutaneous leishmaniasis.

Methods

This Q-methodology study was carried out in Erbil, Kurdistan Region of Iraq, and involved 37 dermatologists. A set of 40 statements related to different aspects of difficulties and uncertainties of treating cutaneous leishmaniasis was prepared. The dermatologists were requested to distribute the 40 statements into a scaled grid of nine piles from least agree to most agree. We applied by-person factor analysis using PQMethod 2.35 for the data analysis.

Results

The analysis revealed two different viewpoints about the treatment of cutaneous leishmaniasis and a consensus viewpoint. The first viewpoint emphasized the use of sodium stibogluconate-based combination therapy, concerns with treatment failure, and lack of compliance with the treatment. The second viewpoint emphasized the lack of standard treatment and advances in the treatment of cutaneous leishmaniasis. There was a consensus between both groups of respondents about many aspects of the treatment of cutaneous leishmaniasis, including considering sodium stibogluconate the first drug of choice for cutaneous leishmaniasis treatment.

Conclusions

This study revealed a diversity of viewpoints and uncertainties about the effectiveness of the available treatment modalities and treatment difficulties and failure. Interrupted supply and poor quality of the available drugs and lack of a standard and advanced treatment are the main problems facing the treatment of cutaneous leishmaniasis. More research is required to determine the best treatment modalities for the different types of cutaneous leishmaniasis. There is a need for the development of treatment guidelines specific to the Iraqi context with a particular focus on the treatment of the resistant and atypical cases of cutaneous leishmaniasis.
Effect of donor non-muscle myosin heavy chain (MYH9) gene polymorphisms on clinically relevant kidney allograft dysfunction
Authors: Pazik Joanna , Oldak Monika , Oziębło Dominika , Materkowska Dominika Dęborska , Sadowska Anna , Malejczyk Jacek , Durlik Magdalena
Published: September 2020

Background

Despite its established association with chronic kidney disease (CKD) the role of myosin-9 (MYH9) gene variation on transplanted kidney function remains unknown. This study aimed at evaluating the effect of donor MYH9 nephrogenic variants on renal allograft function within the first post transplantation year.

Methods

In the longitudinal kidney transplant study 207 deceased donors were genotyped for previously known risk MYH9 single nucleotide polymorphisms (SNPs). The predictor was MYH9 high–risk variants status. The primary outcome was mean eGFR found in low vs. high risk MYH9 genotypes between third and twelfth post-transplant month, the secondary outcome was the risk of proteinuria.

Results

Distribution of genotypes remained in Hardy-Weinberg equilibrium. The T allele of rs3752462 (dominant model, TT or TC vs. CC) was associated with higher filtration rate (P = 0.05) in a multivariate analysis after adjusting for delayed graft function and donor sex. Two G alleles of rs136211 (recessive model, GG vs. GA or AA) resulted in doubling the risk of proteinuria (OR = 2.22; 95% CI = 1.18–4.37, P = 0.017) after adjusting for donor and recipient sex.

Conclusion

Deceased donor kidneys of European descent harboring MYH9 SNPs rs3752462 T allele show significantly superior estimated filtration rate while those of rs136211 GG genotype excessive risk of proteinuria. These findings, if replicated, may further inform and improve individualization of allocation and treatment policies.
Brain hypoperfusion and nigrostriatal dopaminergic dysfunction in primary familial brain calcification caused by novel MYORG variants: case report
Authors: Chen Shih-Ying , Lin Wei-Che , Chang Yung-Yee , Lin Tsu-Kung , Lan Min-Yu
Published: September 2020

Background

Primary familial brain calcification (PFBC) is a rare inherited disease characterized by multiple calcified foci in the brain parenchyma. MYORG is the first gene found to be associated with autosomal recessive PFBC. The precise pathogenic mechanism of neurodegeneration in PFBC remains unclear. The clinical phenotypes of PFBC are variable, and there is no clear correlation between clinical manifestations and radiological and pathological features of calcification.

Case presentation

Two sisters in a Taiwanese family presented with young-onset Parkinsonism and multifocal dystonia. Their brain CTs showed multiple intracerebral calcifications. The genetic study detected two heterozygous novel variants, c.104 T > A (p.Met35Lys) and c.850 T > C (p.Cys284Arg) in the MYORG gene. In both patients, MR susceptibility weighted images revealed calcification of the deep medullary veins. Tc99m ECD SPECT demonstrated a significant decrease of tracer uptake in the brain cortex and subcortical gray matter. Tc99m TRODAT-1 SPECT revealed decreased tracer uptake in the bilateral striatum.

Conclusion

Two novel MYORG variants were identified in Taiwanese family members presenting with PFBC. Abnormalities in the brain perfusion and dopamine transporter SPECTs suggest that cerebral ischemia due to extensive calcified vasculopathy, disruption of the basal ganglia-thalamo-cortical circuit, and nigrostriatal dopaminergic dysfunction are plausible pathogenic mechanisms of neurodegeneration in PFBC patients. Further investigation into the correlations between the pathogenicity-implicated imaging findings and the clinical phenotype are recommended.
Increased neutrophil-to-lymphocyte ratio predicts the development of post-stroke infections in patients with acute ischemic stroke
Authors: He Lanying , Wang Jian , Wang Feng , Zhang Lili , Zhang Lijuan , Zhao Wang
Published: September 2020

Background

Infections could increase the risk of poor outcome in patients with acute ischemic stroke (AIS). The peripheral neutrophil-to-lymphocyte ratio (NLR) is an important indicator of inflammation. The purpose of our study was to investigate the association increased NLR with post stroke infections (PSI) in AIS.

Methods

In this study, we included 606 consecutive patients with AIS within 24 h. The NLR was calculated by dividing absolute neutrophil counts by absolute lymphocyte counts. Receiver operating characteristic (ROC) curve was performed to identify the optimal cut point of NLR for PSI. The relationship between NLR and PSI was analyzed by multivariable analysis.

Results

We assessed 606 consecutive patients with AIS. ROC curve analysis showed that the optimal cut point of NLR for PSI was NLR ≥ 5.79. Compared with no PSI, patients with PSI have higher NLR, older age, higher NIHSS, higher PCT, higher percentage of nasogastric tube feeding and indwelling urinary catheter (P < 0.05). Multivariable analysis showed that NLR ≥ 5.79 [adjusted odds ratio (aOR),4.52; 95% confidence interval (CI),3.02–6.76; P < 0.001], older age (aOR,1.03; 95% CI, 1.00–1.05; P = 0.009), higher admission NIHSS (aOR,1.13; 95%CI, 1.07–1.18; P < 0.001), indwelling urinary catheter (aOR1.83; 95%CI, 1.08–3.10; P = 0.026], and nasogastric tube feeding (aOR2.52; 95%CI, 1.38–4.59; P = 0.003) were associated with increased risk of PSI.

Conclusions

Higher NLR can predict PSI in AIS patients. The NLR may help to select high-risk patients to start intervention in time.
Antenatal depression and anxiety and early pregnancy BMI among White British and South Asian women: retrospective analysis of data from the Born in Bradford cohort
Authors: Insan Nafisa , Slack Emma , Heslehurst Nicola , Rankin Judith
Published: September 2020

Background

Maternal obesity has severe physical impacts such as increased chances of pre-eclampsia and gestational diabetes. However, mental health impacts are given less attention within antenatal care. Evidence suggests that women with obesity carry increased risk of maternal depression and anxiety, however, this association is not well researched amongst South Asian women in the UK who are vulnerable to both. The aim of this study was to investigate the association between antenatal depression and anxiety and early pregnancy BMI, within and between White British and South Asian women, using data from the Born in Bradford cohort.

Methods

Depression and anxiety were assessed using the General Health Questionnaire (GHQ); a GHQ score of > 0 for the depression subscale and > 6 for anxiety. Mother’s BMI was stratified into six World Health Organisation BMI categories (underweight, recommended, overweight or obese class 1–3). To determine associations, univariate and multivariate logistic regression models (adjusting for maternal age, education, deprivation and smoking) were used.

Results

There were 7824 women included (3514 White British and 4310 South Asian). South Asian women were more likely to have depression than White British (43.3% vs 36.1% p < 0.0001) and less likely to have anxiety (45.3% vs 48.4% p < 0.01). There were no significant associations between BMI and depression or anxiety in South Asian women. White British women with an overweight BMI had higher odds of anxiety compared with women with a recommended BMI (Adjusted Odds Ratio 1.25, 95% Confidence Interval 1.05–1.47). No significant associations were observed for other BMI categories. Smoking was a risk factor for antenatal depression (AOR 1.32, 95% CI 1.12–1.56; AOR 2.08, 95% CI 1.49–2.91) and anxiety (AOR 1.34, 95% CI 1.14–1.57; (AOR 2.87, 95% CI 2.02–4.07) in both White British and South Asian women, respectively.

Conclusions

Although South Asian women have a higher prevalence of depression than White women in this cohort, the known associations between maternal obesity and anxiety do not appear to be present. More studies are needed using validated depression tools for South Asian pregnant women. Mental health screening during antenatal care is important for South Asian women, with factors such as smoking considered.