bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Macular and Peripapillary Blood Flow in Response to Intraocular Pressure Reduction in Patients With Posner–Schlossman Syndrome
Authors: Liu Dan , Fan Cong , Zhang Endong , Yang Jie , Zhang Yue , Jiang Jian
Published: June 2022

Purpose: The study aimed to evaluate the effect of intraocular pressure (IOP) reduction on macular and peripapillary microcirculation in patients with Posner–Schlossman syndrome (PSS) by optical coherence tomography angiography (OCTA).

Methods: A prospective comparative study was conducted. Patients diagnosed with PSS at the Eye Center of Xiangya Hospital, Central South University, from February 2020 to November 2021 were consecutively included. OCTA was used for the macular and peripapillary microcirculation measurements, and optical coherence tomography (OCT) was employed for the retinal nerve fiber layer (RNFL) and lamina cribrosa depth (LCD) measurements. The patients received OCT and OCTA examinations at baseline and 1 week post-treatment when the IOP was under control. Changes in macular and peripapillary microcirculation, RNFL, and LCD were calculated for all the analyzed areas.

Results: Twenty-one eyes from 21 patients were included in the study. Pre-treatment and post-treatment IOP were 43.17 ± 10.36 mm Hg (range, 30–60 mm Hg) and 17.17 ± 2.85 mm Hg (range, 13–23 mm Hg), respectively. No statistically significant changes were detected in RNFL, LCD, or macular and peripapillary microcirculation after significant IOP reduction.

Conclusion: The results suggested that a large IOP reduction may not result in a significant increase in peripapillary and macular capillary perfusion in patients with PSS.
Caterpillar-Induced Rice Volatile (E)-β-Farnesene Impairs the Development and Survival of Chilo suppressalis Larvae by Disrupting Insect Hormone Balance
Authors: Yang Lei , Yao Xiaomin , Liu Baosheng , Han Yangchun , Ji Rui , Ju Jiafei , Zhang Xiaona , Wu Shuwen , Fang Jichao , Sun Yang
Published: June 2022

Significant research progress has recently been made on establishing the roles of tps46 in rice defense. (E)-β-farnesene (Eβf) is a major product of tps46 activity but its physiological functions and potential mechanisms against Chilo suppressalis have not yet been clarified. In the present study, C. suppressalis larvae were artificially fed a diet containing 0.8 g/kg Eβf and the physiological performance of the larvae was evaluated. In response to Eβf treatment, the average 2nd instar duration significantly increased from 4.78 d to 6.31 d while that of the 3rd instar significantly increased from 5.70 d to 8.00 d compared with the control. There were no significant differences between the control and Eβf-fed 4th and 5th instars in terms of their durations. The mortalities of the 2nd and 3rd Eβf-fed instars were 21.00-fold and 6.39-fold higher, respectively, than that of the control. A comparative transcriptome analysis revealed that multiple differentially expressed genes are involved in insect hormone biosynthesis. An insect hormone assay on the 3rd instars disclosed that Eβf disrupted the balance between the juvenile hormone and ecdysteroid levels. Eβf treatment increased the juvenile hormones titers but not those of the ecdysteroids. The qPCR results were consistent with those of the RNA-Seq. The foregoing findings suggested that Eβf impairs development and survival in C. suppressalis larvae by disrupting their hormone balance. Moreover, Eβf altered the pathways associated with carbohydrate and xenobiotic metabolism as well as those related to cofactors and vitamins in C. suppressalis larvae. The discoveries of this study may contribute to the development and implementation of an integrated control system for C. suppressalis infestations in rice.
A Novel In Silico Electromechanical Model of Human Ventricular Cardiomyocyte
Authors: Bartolucci Chiara , Forouzandehmehr Mohamadamin , Severi Stefano , Paci Michelangelo
Published: June 2022

Contractility has become one of the main readouts in computational and experimental studies on cardiomyocytes. Following this trend, we propose a novel mathematical model of human ventricular cardiomyocytes electromechanics, BPSLand, by coupling a recent human contractile element to the BPS2020 model of electrophysiology. BPSLand is the result of a hybrid optimization process and it reproduces all the electrophysiology experimental indices captured by its predecessor BPS2020, simultaneously enabling the simulation of realistic human active tension and its potential abnormalities. The transmural heterogeneity in both electrophysiology and contractility departments was simulated consistent with previous computational and in vitro studies. Furthermore, our model could capture delayed afterdepolarizations (DADs), early afterdepolarizations (EADs), and contraction abnormalities in terms of aftercontractions triggered by either drug action or special pacing modes. Finally, we further validated the mechanical results of the model against previous experimental and in silico studies, e.g., the contractility dependence on pacing rate. Adding a new level of applicability to the normative models of human cardiomyocytes, BPSLand represents a robust, fully-human in silico model with promising capabilities for translational cardiology.
Leaf Functional Traits of Invasive Grasses Conferring High-Cadmium Adaptation Over Natives
Authors: Ilyas Muhammad , Shah Sakhawat , Lai Ya-Wen , Sher Jan , Bai Tao , Zaman Fawad , Bibi Farkhanda , Koul Monika , Wani Shabir Hussain , Majrashi Ali , Alharby Hesham F. , Hakeem Khalid Rehman , Wang Yong-Jian , Rather Shabir A.
Published: June 2022

Heavy metal (HM) contamination resulting from industrialization and urbanization during the Anthropocene along with plant invasion can severely threaten the growth and adaptation of local flora. Invasive alien plant species generally exhibit a growth pattern consistent with their functional traits in non-contaminated environments in the introduced range. However, it remains unclear whether invasive alien plants have an advantage over native plants in contaminated environments and whether this growth pattern is dependent on the adaptation of their leaf functional traits. Here, we selected two congeneric pairs of invasive alien and native grasses that naturally co-exist in China and are commonly found growing in contaminated soil. To evaluate the effect of cadmium (Cd) on the structural and physiological leaf traits, we grew all four species in soil contaminated without or with 80 mg/kg Cd. Invasive plants contained significantly higher concentrations of Cd in all three organs (leaf, stem, and root). They displayed a higher transfer factor and bioconcentration factor (BCF) of shoot and root than natives, indicating that invasive species are potential Cd hyperaccumulators. Invasive plants accumulated polyphenol oxidase (PPO) to higher levels than natives and showed similar patterns of leaf structural and physiological traits in response to changes in Cd bioconcentration. The quantifiable leaf structural traits of invasive plants were significantly greater (except for stomatal density and number of dead leaves) than native plants. Leaf physiological traits, chlorophyll content, and flavonoid content were also significantly higher in invasive plants than in natives under Cd stress conditions after 4 weeks, although nitrogen balance index (NBI) showed no significant difference between the two species. Chlorophyll fluorescence parameters decreased, except for the quantum yield of photosystem II (ΦPSII) and the proportion of open photosystem II (qP), which increased under Cd stress conditions in both species. However, invasive plants exhibited higher fluorescence parameters than natives under Cd stress, and the decrement observed in invasive plants under Cd stress was greater than that in natives. High Cd adaptation of invasive grasses over natives suggests that invasive plants possess optimal leaf structural and physiological traits, which enable them to adapt to stressful conditions and capture resources more quickly than natives. This study further emphasizes the potential invasion of alien plants in contaminated soil environments within the introduced range. To a certain extent, some non-invasive alien plants might adapt to metalliferous environments and serve as hyperaccumulator candidates in phytoremediation projects in contaminated environments.
TaPR1 Interacts With TaTLP1 via the αIV Helix to Be Involved in Wheat Defense to Puccinia triticina Through the CAPE1 Motif
Authors: Wang Fei , Shen Songsong , Zhao Cunpeng , Cui Zhongchi , Meng Linshuo , Wu Wenyue , Liu Daqun , Wang Haiyan
Published: June 2022

Pathogenesis-related (PR) proteins play important roles in plant defense response and systemic acquired resistance (SAR). PR1 has antifungal activity against many plant pathogens. In our previous study, RNA sequencing (RNA-seq) was conducted on resistant wheat line TcLr19 and sensitive wheat cultivar Chinese Spring inoculated with Puccinia triticina (Pt) race PHNT. In this study, seven salicylic acid (SA)-induced TaPR1 genes involved in plant disease resistance were found in the RNA-seq library. Quantitative PCR (qPCR) results showed that TaPR1-4 was most induced by Pt among these seven TaPR1 genes in the incompatible interaction. Yeast two-hybrid (Y2H) results showed that TaPR1-4 interacted with TaTLP1 via the αIV helix. Protein-mediated phenotyping assays in vivo and antifungal activity in vitro demonstrated that wheat leaves infiltrated with pure TaPR1-4 protein developed significantly less disease compared to control leaves. This effect was correlated with a strong increase in defense gene expression, and resistance activity was dependent on the CAPE1 motif located in the C-terminal region of TaPR1-4. These findings increase current knowledge regarding the interaction of TaPR1 and TaTLP1 and provide new insights on the role of TaPR1 protein in the resistance of wheat to Pt.
Role of Rhizospheric Bacillus megaterium HGS7 in Maintaining Mulberry Growth Under Extremely Abiotic Stress in Hydro-Fluctuation Belt of Three Gorges Reservoir
Authors: Ou Ting , Zhang Meng , Huang Yazhou , Wang Li , Wang Fei , Wang Ruolin , Liu Xiaojiao , Zhou Zeyang , Xie Jie , Xiang Zhonghuai
Published: June 2022

Plant growth-promoting rhizobacteria have been shown to play important roles in maintaining host fitness under periods of abiotic stress, and yet their effect on mulberry trees which regularly suffer drought after flooding in the hydro-fluctuation belt of the Three Gorges Reservoir Region in China remains largely uncharacterized. In the present study, 74 bacterial isolates were obtained from the rhizosphere soil of mulberry after drought stress, including 12 phosphate-solubilizing and 10 indole-3-acetic-acid-producing isolates. Bacillus megaterium HGS7 was selected for further study due to the abundance of traits that might benefit plants. Genomic analysis revealed that strain HGS7 possessed multiple genes that contributed to plant growth promotion, stress tolerance enhancement, and antimicrobial compound production. B. megaterium HGS7 consistently exhibited antagonistic activity against phytopathogens and strong tolerance to abiotic stress in vitro. Moreover, this strain stimulated mulberry seed germination and seedling growth. It may also induce the production of proline and antioxidant enzymes in mulberry trees to enhance drought tolerance and accelerate growth recovery after drought stress. The knowledge of the interactions between rhizobacteria HGS7 and its host plant might provide a potential strategy to enhance the drought tolerance of mulberry trees in a hydro-fluctuation belt.
Non-destructive Technologies for Plant Health Diagnosis
Authors: Ang Mervin Chun-Yi , Lew Tedrick Thomas Salim
Published: June 2022

As global population grows rapidly, global food supply is increasingly under strain. This is exacerbated by climate change and declining soil quality due to years of excessive fertilizer, pesticide and agrichemical usage. Sustainable agricultural practices need to be put in place to minimize destruction to the environment while at the same time, optimize crop growth and productivity. To do so, farmers will need to embrace precision agriculture, using novel sensors and analytical tools to guide their farm management decisions. In recent years, non-destructive or minimally invasive sensors for plant metabolites have emerged as important analytical tools for monitoring of plant signaling pathways and plant response to external conditions that are indicative of overall plant health in real-time. This will allow precise application of fertilizers and synthetic plant growth regulators to maximize growth, as well as timely intervention to minimize yield loss from plant stress. In this mini-review, we highlight in vivo electrochemical sensors and optical nanosensors capable of detecting important endogenous metabolites within the plant, together with sensors that detect surface metabolites by probing the plant surface electrophysiology changes and air-borne volatile metabolites. The advantages and limitations of each kind of sensing tool are discussed with respect to their potential for application in high-tech future farms.
Deciphering the Molecular Signatures Associated With Resistance to Botrytis cinerea in Strawberry Flower by Comparative and Dynamic Transcriptome Analysis
Authors: Xiao Guilin , Zhang Qinghua , Zeng Xiangguo , Chen Xiyang , Liu Sijia , Han Yongchao
Published: June 2022

Gray mold caused by Botrytis cinerea, which is considered to be the second most destructive necrotrophic fungus, leads to major economic losses in strawberry (Fragaria × ananassa) production. B. cinerea preferentially infects strawberry flowers and fruits, leading to flower blight and fruit rot. Compared with those of the fruit, the mechanisms of flower defense against B. cinerea remain largely unexplored. Therefore, in this study, we aimed to unveil the resistance mechanisms of strawberry flower through dynamic and comparative transcriptome analysis with resistant and susceptible strawberry cultivars. Our experimental data suggest that resistance to B. cinerea in the strawberry flower is probably regulated at the transcriptome level during the early stages of infection and strawberry flower has highly complex and dynamic regulatory networks controlling a multi-layered defense response to B. cinerea. First of all, the higher expression of disease-resistance genes but lower expression of cell wall degrading enzymes and peroxidases leads to higher resistance to B. cinerea in the resistant cultivar. Interestingly, CPKs, RBOHDs, CNGCs, and CMLs comprised a calcium signaling pathway especially play a crucial role in enhancing resistance by increasing their expression. Besides, six types of phytohormones forming a complex regulatory network mediated flower resistance, especially JA and auxin. Finally, the genes involved in the phenylpropanoid and amino acids biosynthesis pathways were gene sets specially expressed or different expression genes, both of them contribute to the flower resistance to B. cinerea. These data provide the foundation for a better understanding of strawberry gray mold, along with detailed genetic information and resistant materials to enable genetic improvement of strawberry plant resistance to gray mold.
Evolution of an Apomixis-Specific Allele Class in Supernumerary Chromatin of Apomictic Boechera
Authors: Mau Martin , Mandáková Terezie M. , Ma Xingliang , Ebersbach Jana , Zou Lifang , Lysak Martin A. , Sharbel Timothy F.
Published: June 2022

Asexual reproduction through seeds in plants (i.e., apomixis) is a heritable trait, and apomixis- linked loci have been identified in multiple species. However, direct identification of genomic elements is typically hindered as apomixis-linked loci and are commonly found in recombination-suppressed and repetitive regions. Heterochromatinized elements, such as B chromosomes and other supernumerary chromosomal DNA fragments have long been known to be associated with asexuality in both plants and animals and are prime candidate regions for the evolution of multiple apomixis factors controlling the individual elements of apomixis. Here, we examined molecular evolution, gene regulation, and chromosomal location of a male apomeiosis factor (UPG2), a long noncoding RNA gene, in sexual and apomictic Boechera with and without male apomeiosis (i.e., balanced and unbalanced apomicts). We revealed the origin of the gene in the apomixis genome on an apomixis-specific, supernumerary heterochromatic Boechera chromosome (Boe1). The UPG2 is active in the tapetum at male meiosis. We found allele classes specific to apomictic and sexual Boechera accessions and a third class that shares the features of both and points to a convergent transition state. Sex alleles are found only in some of the sexual accessions and have higher nucleotide divergence and lower transcriptional activity compared to apo alleles. These data demonstrate selective pressure to maintain the function of UPG2 for unreduced pollen formation in apomicts as the occasional transmission of the allele from unbalanced apomicts into sexual organisms that lead to pseudogenization and functional decay of copies in sexual organisms.
Genome-Wide Identification and Expression Pattern Analysis of KNOX Gene Family in Orchidaceae
Authors: Zhang Diyang , Lan Siren , Yin Wei-Lun , Liu Zhong-Jian
Published: June 2022

The establishment of lateral organs and subsequent plant architecture involves factors intrinsic to the stem apical meristem (SAM) from which they are derived. KNOTTED1-LIKE HOMEOBOX (KNOX) genes are a family of plant-specific homeobox transcription factors that especially act in determining stem cell fate in SAM. Although KNOXs have been studied in many land plants for decades, there is a dearth of knowledge on KNOX’s role in Orchidaceae, the largest and most diverse lineage of flowering plants. In this study, a total of 32 putative KNOX genes were identified in the genomes of five orchid species and further designated into two classes (Class I and Class II) based on phylogenetic relationships. Sequence analysis showed that most orchid KNOX proteins retain four conserved domains (KNOX1, KNOX2, ELK, and Homeobox_KN). Comparative analysis of gene structure showed that the exon–intron structure is conserved in the same clade but most orchids exhibited longer intron, which may be a unique feature of Orchidaceae. Cis-elements identified in the promoter region of orchid KNOXs were found mostly enriched in a function of light responsiveness, followed by MeJA and ABA responsiveness, indicative of their roles in modulating light and phytohormones. Collinear analysis unraveled a one-to-one correspondence among KNOXs in orchids, and all KNOX genes experienced strong purifying selection, indicating the conservation of this gene family has been reinforced across the Orchidaceae lineage. Expression profiles based on transcriptomic data and real-time reverse transcription–quantitative PCR (RT-qPCR) revealed a stem-specific expression of KNOX Class I genes and a broader expression pattern of Class II genes. Taken together, our results provided a comprehensive analysis to uncover the underlying function of KNOX genes in Orchidaceae.
Effects of Water Availability on the Relationships Between Hydraulic and Economic Traits in the Quercus wutaishanica Forests
Authors: Zhang Yuhan , Zhao Jiale , Xu Jinshi , Chai Yongfu , Liu Peiliang , Quan Jiaxin , Wu Xipin , Li Cunxia , Yue Ming
Published: June 2022

Water availability is a key environmental factor affecting plant species distribution, and the relationships between hydraulic and economic traits are important for understanding the species’ distribution patterns. However, in the same community type but within different soil water availabilities, the relationships in congeneric species remain ambiguous. In northwest China, Quercus wutaishanica forests in the Qinling Mountains (QM, humid region) and Loess Plateau (LP, drought region) have different species composition owing to contrasting soil water availability, but with common species occurring in two regions. We analyzed eight hydraulic traits [stomatal density (SD), vein density (VD), wood specific gravity (WSGbranch), lower leaf area: sapwood area (Al: As), stomatal length (SL), turgor loss point (ΨTlp), maximum vessel diameter (Vdmax) and height (Height)] and five economic traits [leaf dry matter content (LDMC), leaf tissue density (TD), leaf dry mass per area (LMA), Leaf thickness (LT) and maximum net photosynthetic rate (Pmax)] of congeneric species (including common species and endemic species) in Q. wutaishanica forests of QM and LP. We explored whether the congeneric species have different economic and hydraulic traits across regions. And whether the relationship between hydraulic and economic traits was determined by soil water availability, and whether it was related to species distribution and congeneric endemic species composition of the same community. We found that LP species tended to have higher SD, VD, WSGbranch, Al: As, SL, ΨTlp and Vdmax than QM species. There was a significant trade-off between hydraulic efficiency and safety across congeneric species. Also, the relationships between hydraulic and economic traits were closer in LP than in QM. These results suggested that relationships between hydraulic and economic traits, hydraulic efficiency and safety played the role in constraining species distribution across regions. Interestingly, some relationships between traits changed (from significant correlation to non-correlation) in common species across two regions (from LP to QM), but not in endemic species. The change of these seven pairs of relationships might be a reason for common species’ wide occurrence in the two Q. wutaishanica forests with different soil water availability. In drought or humid conditions, congeneric species developed different types of adaptation mechanisms. The study helps to understand the environmental adaptive strategies of plant species, and the results improve our understanding of the role of both hydraulic and economic traits during community assembly.
The Role of Genetic Factors in the Differential Invasion Success of Two Spartina Species in China
Authors: Li Feifei , Liu Xiaoyan , Zhu Jinfang , Li Junsheng , Gao Kexiao , Zhao Caiyun
Published: June 2022

Biological invasions have become one of the greatest threats to global biodiversity and ecosystem conservation. Most previous studies have revealed how successful invasive species adapt to new environments and climate change through phenotypic and genetic evolution. Some researchers suggested that understanding unsuccessful or less successful biological invasions might be important for understanding the relationships between invasion adaptability and climate factors. We compared the sexual reproduction ability, genetic diversity, and gene × environment interaction in two intentionally introduced alien species in China (Spartina anglica and Spartina alterniflora) based on restriction site-associated DNA (RAD) sequencing. After more than 50 years, the distribution of S. alterniflora has rapidly expanded, while S. anglica has experienced extreme dieback. A total of 212,939 single nucleotide polymorphisms (SNPs) for the two Spartina species were used for analysis. The multilocus genotype (MLG) analysis revealed that clonal reproduction was the prevalent mode of reproduction in both species, indicating that a change in the mode of reproduction was not the key factor enabling successful invasion by Spartina. All genetic diversity indicators (He, Ho, π) in S. alterniflora populations were at least two times higher than those in S. anglica populations, respectively (p < 0.001). Furthermore, the population genetic structure and stronger patterns of climate-associated loci provided support for rapid adaptive evolution in the populations of S. alterniflora in China. Altogether, our results highlight the importance of genetic diversity and local adaptation, which were driven by multiple source populations, in increasing the invasiveness of S. alterniflora.
Orchid Phylotranscriptomics: The Prospects of Repurposing Multi-Tissue Transcriptomes for Phylogenetic Analysis and Beyond
Authors: Wong Darren C. J. , Peakall Rod
Published: June 2022

The Orchidaceae is rivaled only by the Asteraceae as the largest plant family, with the estimated number of species exceeding 25,000 and encompassing more than 700 genera. To gain insights into the mechanisms driving species diversity across both global and local scales, well-supported phylogenies targeting different taxonomic groups and/or geographical regions will be crucial. High-throughput sequencing technologies have revolutionized the field of molecular phylogenetics by simplifying the process of obtaining genome-scale sequence data. Consequently, there has been an explosive growth of such data in public repositories. Here we took advantage of this unprecedented access to transcriptome data from predominantly non-phylogenetic studies to assess if it can be repurposed to gain rapid and accurate phylogenetic insights across the orchids. Exhaustive searches revealed transcriptomic data for more than 100 orchid species spanning 5 subfamilies, 13 tribes, 21 subtribes, and 50 genera that were amendable for exploratory phylotranscriptomic analysis. Next, we performed re-assembly of the transcriptomes before strategic selection of the final samples based on a gene completeness evaluation. Drawing on these data, we report phylogenetic analyses at both deep and shallow evolutionary scales via maximum likelihood and shortcut coalescent species tree methods. In this perspective, we discuss some key outcomes of this study and conclude by highlighting other complementary, albeit rarely explored, insights beyond phylogenetic analysis that repurposed multi-tissue transcriptome can offer.
WRKY Genes Improve Drought Tolerance in Arachis duranensis
Authors: Zhang Yongli , Du Pei , Xiong Faqian , Zhang Xiaojun , Song Hui
Published: June 2022

WRKY transcription factor participates in plant growth and development and response to biotic and abiotic stresses. Arachis duranensis, a turfgrass, has high drought tolerance, yet little is known about AdWRKYs response to drought stress in A. duranensis. In this study, RNA-seq identified five AdWRKYs, including AdWRKY18, AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, which were upregulated under drought stress. Orthologous relationships between AdWRKYs and Arabidopsis WRKY were determined to predict the regulatory networks of the five AdWRKYs based on AtWRKYs. Additionally, protein–protein interactions were predicted using differentially expressed proteins from RNA-seq. The quantitative real-time PCR (qRT-PCR) results showed that AdWRKY40 was upregulated, while AdWRKY42, AdWRKY56, and AdWRKY64 were downregulated at different time-points under drought stress. The predicted regulatory networks showed that AdWRKY40 activates COR47, RD21, and RD29A expression under drought stress. Besides, AdWRKY56 regulated CesA8 under drought stress. Aradu.YIQ80 (NAC019) interacted with AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, while Aradu.Z5H58 (NAC055) interacted with AdWRKY42 and AdWRKY64 under drought stress. This study used Arabidopsis to assess AdWRKYs function and regulatory networks, providing a basis for understanding drought tolerance in A. duranensis.
Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses
Authors: Zhou Qiying , Zhao Mingwei , Xing Feng , Mao Guangzhi , Wang Yijia , Dai Yafeng , Niu Minghui , Yuan Hongyu
Published: June 2022

Calmodulin-binding transcription activators (CAMTAs) are evolutionarily conserved transcription factors and have multi-functions in plant development and stress response. However, identification and functional analysis of tea plant (Camellia sinensis) CAMTA genes (CsCAMTAs) are still lacking. Here, five CsCAMTAs were identified from tea plant genomic database. Their gene structures were similar except CsCAMTA2, and protein domains were conserved. Phylogenetic relationship classified the CsCAMTAs into three groups, CsCAMTA2 was in group I, and CsCAMTA1, 3 and CsCAMTA4, 5 were, respectively, in groups II and III. Analysis showed that stress and phytohormone response-related cis-elements were distributed in the promoters of CsCAMTA genes. Expression analysis showed that CsCAMTAs were differentially expressed in different organs and under various stress treatments of tea plants. Three-hundred and four hundred-one positive co-expressed genes of CsCAMTAs were identified under cold and drought, respectively. CsCAMTAs and their co-expressed genes constituted five independent co-expression networks. KEGG enrichment analysis of CsCAMTAs and the co-expressed genes revealed that hormone regulation, transcriptional regulation, and protein processing-related pathways were enriched under cold treatment, while pathways like hormone metabolism, lipid metabolism, and carbon metabolism were enriched under drought treatment. Protein interaction network analysis suggested that CsCAMTAs could bind (G/A/C)CGCG(C/G/T) or (A/C)CGTGT cis element in the target gene promoters, and transcriptional regulation might be the main way of CsCAMTA-mediated functional regulation. The study establishes a foundation for further function studies of CsCAMTA genes in stress response.
AmCBF1 Transcription Factor Regulates Plant Architecture by Repressing GhPP2C1 or GhPP2C2 in Gossypium hirsutum
Authors: Lu Junchao , Wang Lihua , Zhang Qianqian , Ma Caixia , Su Xiaofeng , Cheng Hongmei , Guo Huiming
Published: June 2022

Dwarfism is a beneficial trait in many crops. Dwarf crops hold certain advantages over taller crops in lodging resistance, fertilizer tolerance, and yield. Overexpression of CBF/DREB transcription factors can lead to dwarfing in many plant species, but the molecular mechanism of plant dwarfing caused by overexpression of CBF/DREB in upland cotton (Gossypium hirsutum) remains unclear. In this study, we observed that overexpression of the Ammopiptanthus mongolicus AmCBF1 transcription factor in upland cotton R15 reduced plant height, whereas virus-induced gene silencing of AmCBF1 in the derived dwarf lines L28 and L30 partially restored plant height. Five protein phosphatase (PP2C) genes (GhPP2C1 to GhPP2C5) in cotton were identified by RNA-sequencing among genes differentially expressed in L28 or L30 in comparison with R15 and thus may play an important role in AmCBF1-regulated dwarfing in cotton. Gene expression analysis showed that the GhPP2C genes were down-regulated significantly in L28 and L30, and silencing of GhPP2C1 or GhPP2C2 in R15 inhibited the growth of cotton seedlings. Subcellular localization assays revealed that GhPP2C1 was localized to the cell membrane and nucleus, whereas GhPP2C2 was exclusively localized to the nucleus. Yeast one-hybrid and dual-luciferase assays showed that AmCBF1 was able to bind to the CRT/DRE elements of the upstream promoter of GhPP2C1 or GhPP2C2 and repress their expression. These findings provide insight into the mechanism of dwarfing and may contribute to the breeding of dwarf cultivars of upland cotton.
Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
Authors: King Julie , Grewal Surbhi , Othmeni Manel , Coombes Benedict , Yang Cai-yun , Walter Nicola , Ashling Stephen , Scholefield Duncan , Walker Jack , Hubbart-Edwards Stella , Hall Anthony , King Ian Phillip
Published: June 2022

Triticum timopheevii (2n = 28, AtAtGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the At and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the At and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3At, 4G, and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting At subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the At segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.
How and When Daily Abusive Supervision Affects Daily Organizational Citizenship Behavior for the Environment
Authors: Wang Hongqing , Xiao Jiuling
Published: June 2022

Organizational environmental sustainability depends primarily on employees’ organizational citizenship behavior for the environment (OCBE), and leadership plays an important role in improving and inhibiting employees’ OCBE. The purpose of the present study is to examine the mediation process by which abusive supervision affects employees’ OCBE through a daily diary study and to explore the boundary conditions of the relationship between daily moral disengagement and daily OCBE. We collected data from 112 Chinese employees for 10 consecutive days. The results show that daily abusive supervision has a significant negative effect on daily OCBE and that daily moral disengagement plays a significant mediating role in this relationship. In addition, the negative effect of daily moral disengagement on daily OCBE could be attenuated by a psychological green climate. Our findings support our hypotheses and offer useful theoretical and practical implications for promoting OCBE.
A Multicriteria Approach for Measuring Employee Well-Being
Authors: Dong Junjie , Yan Shumin
Published: June 2022

This paper proposes that employee well-being includes four dimensions: job satisfaction, life satisfaction, positive affect, and negative affect. Each dimension is interdependent and correlated. Therefore, the measurement of employee well-being is complicated and fuzzy. This study aims to treat the measurement of employee well-being as a fuzzy problem, construct a measurement model from the perspective of multi-criteria decision making, and establish the preference relationship between indicators through fuzzy measure and Choquet integral. Applying multiple linear regression analysis and the heuristic least mean squares method, the main findings are as follows: (1) It is inappropriate to use job satisfaction as a substitute for measuring employee well-being, as the weight of job satisfaction is the lowest among the four dimensions. (2) Employee well-being is also largely reflected in their overall satisfaction with life because life satisfaction is the most heavily weighted. (3) Employee well-being needs to consider the emotion-related indicators and satisfaction-related indicators comprehensively because fuzzy analysis proves that their relationship is redundant. Finally, the practical implications of these findings and future research directions are discussed.
Does Technical Match Performance in Professional Soccer Depend on the Positional Role or the Individuality of the Player?
Authors: Forcher Leon , Forcher Leander , Härtel Sascha , Jekauc Darko , Wäsche Hagen , Woll Alexander , Gross Timo , Altmann Stefan
Published: June 2022

The aim of the study was to examine the impact of the positional role and the individuality on the technical match performance in professional soccer players. From official match data of the Bundesliga season 2018/19, technical performance [short (<10 m)/medium (10–30 m)/long (>30 m) passes, dribblings, ball possessions] of all players who played during the season were analyzed (normative data). Five playing positions (center back, full back, central midfielder, wide midfielder and forward) were distinguished. As the contextual factor tactical formation is known to influence match performance, this parameter was controlled for. Further, those players who played at minimum four games in at least two different playing positions were included in the study sample (n = 13). The technical match performance of the players was analyzed in relation to the normative data regarding the extent to which the players either adapted or maintained their performance when changing the playing position. When switching playing positions, positional role could explain 3–6% of the variance in short passes and ball possessions and 27–44% of the variance in dribblings, medium passes, and long passes. Moreover, we observed large interindividual differences in the extent to which a player changed, adapted, or maintained his performance. In detail, five players clearly adapted their technical performance when changing playing positions, while five players maintained their performance. Coaches can use these findings to better understand the technical match performance of single players and further, to estimate the impact of a change in the positional role on the technical performance of the respective player.
Capturing Sequences of Learners' Self-Regulatory Interactions With Instructional Material During Game-Based Learning Using Auto-Recurrence Quantification Analysis
Authors: Dever Daryn A. , Amon Mary Jean , Vrzáková Hana , Wiedbusch Megan D. , Cloude Elizabeth B. , Azevedo Roger
Published: June 2022

Undergraduate students (N = 82) learned about microbiology with Crystal Island, a game-based learning environment (GBLE), which required participants to interact with instructional materials (i.e., books and research articles, non-player character [NPC] dialogue, posters) spread throughout the game. Participants were randomly assigned to one of two conditions: full agency, where they had complete control over their actions, and partial agency, where they were required to complete an ordered play-through of Crystal Island. As participants learned with Crystal Island, log-file and eye-tracking time series data were collected to pinpoint instances when participants interacted with instructional materials. Hierarchical linear growth models indicated relationships between eye gaze dwell time and (1) the type of representation a learner gathered information from (i.e., large sections of text, poster, or dialogue); (2) the ability of the learner to distinguish relevant from irrelevant information; (3) learning gains; and (4) agency. Auto-recurrence quantification analysis (aRQA) revealed the degree to which repetitive sequences of interactions with instructional material were random or predictable. Through hierarchical modeling, analyses suggested that greater dwell times and learning gains were associated with more predictable sequences of interaction with instructional materials. Results from hierarchical clustering found that participants with restricted agency and more recurrent action sequences had greater learning gains. Implications are provided for how learning unfolds over learners' time in game using a non-linear dynamical systems analysis and the extent to which it can be supported within GBLEs to design advanced learning technologies to scaffold self-regulation during game play.
Burnout Profiles Among Young Researchers: A Latent Profile Analysis
Authors: Boone Anke , Vander Elst Tinne , Vandenbroeck Sofie , Godderis Lode
Published: June 2022

Introduction

Burnout is a growing problem among young researchers, affecting individuals, organizations and society. Our study aims to identify burnout profiles and highlight the corresponding job demands and resources, resulting in recommendations to reduce burnout risk in the academic context.

Methods

This cross-sectional study collected data from young researchers (n = 1,123) at five Flemish universities through an online survey measuring burnout risk, work engagement, sleeping behavior, and the most prominent job demands (e.g., publication pressure) and resources (e.g., social support). We conducted Latent Profile Analysis (LPA) to identify burnout profiles in young researchers and subsequently compared these groups on job demands and resources patterns.

Results

Five burnout profiles were identified: (1) High Burnout Risk (9.3%), (2) Cynical (30.1%), (3) Overextended (2.3%), (4) Low Burnout Risk (34.8%), and (5) No Burnout Risk (23.6%). Each burnout profile was associated with a different pattern of job demands and resources. For instance, high levels of meaningfulness (OR = −1.96) decreased the odds to being classified in the Cynical profile.

Conclusion

Our findings show that the Cynical profile corresponds to a relatively high number of young researchers, which may imply that they are particularly vulnerable to the cynicism dimension of burnout. Additionally, work-life interference and perceived publication pressure seemed the most significant predictors of burnout risk, while meaningfulness, social support from supervisor and learning opportunities played an important protective role.
Relationship Between Family Socioeconomic Status and Learning Burnout of College Students: The Mediating Role of Subjective Well-Being and the Moderating Role of Resilience
Authors: Wu Wenzhi , Liu Yilin , Yu Lei , Guo Zhichao , Li Shujun , Guo Zeyi , Cao Xiang , Tu Fangjun , Wu Xiaoqin , Ma Xiao , Long Qing , Zhao Xinling , Li Xiujuan , Chen Yatang , Zeng Yong
Published: June 2022

Objective

Learning burnout affects the positive development of college students. The present study aimed to investigate the relationship between family socioeconomic status (FSES) and learning burnout, as well as the mediation effect of subjective well-being and the moderation effect of resilience in this relation.

Methods

A total of 550 Chinese college students from Yunnan completed a questionnaire measuring the research variables in this study.

Results

(1) After controlling for participants’ gender and age, FSES negatively, and significantly predicted learning burnout; (2) subjective well-being partially mediated the relationship between FSES and learning burnout; and (3) the direct effect of FSES on learning burnout and the mediation effect of subjective well-being was moderated by resilience. The level of learning burnout of individuals with low resilience increased significantly with the decrease of FSES, and the level of learning burnout of individuals with high resilience decreased significantly with the increase in subjective well-being.

Conclusion

The present findings support the moderated mediation model underlying the relationship between FSES and learning burnout. This also has significant implications for formulating prevention and intervention measures on learning burnout among college students.

Limitations

First of all, this study used the cross-sectional study design, which cannot make a causal inference. In addition, the sample in this study is university students from Kunming, which may affect the popularity of the results.
How Ice Rink Locations Affect Performance Time in Short-Track Speed Skating
Authors: Sun Lixin , Guo Tianxiao , Liu Fei , Tao Kuan
Published: June 2022

Purpose

To accurately provide evaluations on how match performance for elite skaters in short track speed skating developed, and whether geographical factors of ice rink locations should be considered apart from technical abilities. We created a dataset containing competition records from the 2013–14 to 2020–21 seasons (500 m event) on the official website.

Methods

One-way ANOVA was applied to statistically analyze whether the best performance times exhibited significant differences in varied hosting cities. Performance–time matrix and multivariate regression model were further established to quantitatively explain how geographical factors (longitude, latitude, altitude, and barometric pressure) affected performance.

Results

Our findings firstly confirmed that the fastest 500 m finishing times varied due to the hosting cities (P = 0.008) and showed that venue locations could boost or impair performance time with the maximum range of 3.6 s. Meanwhile, latitude (slightly over 46° when performance is maximized) was the most influential factor to account for the performance–time difference in different ice rink locations according to the multivariate regression model, though altitude (1,225 meters when performance is maximized) was also important.

Conclusions

In this perspective, elite skaters should check the geographical factors of the venues before they participated in the upcoming competitions, assess the real strength of their rivals, and adopt flexible tactics during training sessions.
Safe Carrying of Heavy Infants Together With Hair Properties Explain Human Evolution
Authors: do Amaral Lia Queiroz
Published: June 2022

As a physicist, my scientific career was interrupted by maternity, and afterward retaken, with a parallel independent personal perspective on human evolution. My previous published contributions are reanalyzed as Hypothesis and Theory. The focus is on safe infant carrying in primates, sexual selection among Hominoidea, fur reduction in hominins, and tensile properties of hominoid hairs, justifying the necessary change to bipedal locomotion from the overwhelming selective pressure of infant survival. The Discussion starts with analysis of existing bias against acceptance of these new ideas, first with rational arguments on bias existing between Exact Sciences and Biological Sciences. A reanalysis of data on elasticity of hominoid hairs is made, based on published differences between statistical analysis of measurements in exact and inexact sciences. A table constructed from the original data on hair elasticity allows a simplified discussion, based on statistics used in Physics in the study of “known samples,” adding extra information to the available data. Published data on hair density in primates and mammals allow the conclusion that hair elastic properties might have evolved correlated to the pressure of safe carrying of heavy infants, with an upper limit of 1 kgf/cm2for safe infant clinging to primate mother’s hair. The Discussion enters then on the main ideological bias, related to the resistance in the academy to the idea that bipedalism could be connected to a “female problem,” that means, that it was not a “male acquisition.” Tripedal walk, occurring naturally among African Apes carrying their newborns, unable to support themselves by ventral clinging, is the natural candidate leading to evolution of bipedal locomotion. Tripedal walk as an intermediate stage to bipedalism was in fact theoretically proposed, but ignoring its role in primate transportation by ape mothers. The Discussion proceeds to a proposal of phylogenetic evolution of Hominoids, the usual focus on the males changes to the role of females with infants, allowing an integrated view on Hominin evolution, with fur reduction and thermoregulation of the naked skin, with subcutaneous insulating fat layer. The model for earliest hominin social structures is based on huddle formation and hormonally defined rites of passage.