bluuebunny/crossref_metadata_2025 huggingface dataset:
Learning cooking algorithm for solving global optimization problems
Authors: Gopi S. , Mohapatra Prabhujit
Published: June 2024

In recent years, many researchers have made a continuous effort to develop new and efficient meta-heuristic algorithms to address complex problems. Hence, in this study, a novel human-based meta-heuristic algorithm, namely, the learning cooking algorithm (LCA), is proposed that mimics the cooking learning activity of humans in order to solve challenging problems. The LCA strategy is primarily motivated by observing how mothers and children prepare food. The fundamental idea of the LCA strategy is mathematically designed in two phases: (i) children learn from their mothers and (ii) children and mothers learn from a chef. The performance of the proposed LCA algorithm is evaluated on 51 different benchmark functions (which includes the first 23 functions of the CEC 2005 benchmark functions) and the CEC 2019 benchmark functions compared with state-of-the-art meta-heuristic algorithms. The simulation results and statistical analysis such as the t-test, Wilcoxon rank-sum test, and Friedman test reveal that LCA may effectively address optimization problems by maintaining a proper balance between exploitation and exploration. Furthermore, the LCA algorithm has been employed to solve seven real-world engineering problems, such as the tension/compression spring design, pressure vessel design problem, welded beam design problem, speed reducer design problem, gear train design problem, three-bar truss design, and cantilever beam problem. The results demonstrate the LCA’s superiority and capability over other algorithms in solving complex optimization problems.
Whole-genome sequencing identifies variants in ANK1, LRRN1, HAS1, and other genes and regulatory regions for stroke in type 1 diabetes
Authors: Antikainen Anni A. , Haukka Jani K. , Kumar Anmol , Syreeni Anna , Hägg-Holmberg Stefanie , Ylinen Anni , Kilpeläinen Elina , Kytölä Anastasia , Palotie Aarno , Putaala Jukka , Thorn Lena M. , Harjutsalo Valma , Groop Per-Henrik , Sandholm Niina ,   the FinnDiane Study Group, Antikainen Anni A. , Haukka Jani K. , Kumar Anmol , Syreeni Anna , Hägg-Holmberg Stefanie , Ylinen Anni , Putaala Jukka , Thorn Lena M. , Harjutsalo Valma , Groop Per-Henrik , Sandholm Niina
Published: June 2024

Individuals with type 1 diabetes (T1D) carry a markedly increased risk of stroke, with distinct clinical and neuroimaging characteristics as compared to those without diabetes. Using whole-exome or whole-genome sequencing of 1,051 individuals with T1D, we aimed to find rare and low-frequency genomic variants associated with stroke in T1D. We analysed the genome comprehensively with single-variant analyses, gene aggregate analyses, and aggregate analyses on genomic windows, enhancers and promoters. In addition, we attempted replication in T1D using a genome-wide association study (N = 3,945) and direct genotyping (N = 3,263), and in the general population from the large-scale population-wide FinnGen project and UK Biobank summary statistics. We identified a rare missense variant on SREBF1 exome-wide significantly associated with stroke (rs114001633, p.Pro227Leu, p-value = 7.30 × 10–8), which replicated for hemorrhagic stroke in T1D. Using gene aggregate analysis, we identified exome-wide significant genes: ANK1 and LRRN1 displayed replication evidence in T1D, and LRRN1, HAS1 and UACA in the general population (UK Biobank). Furthermore, we performed sliding-window analyses and identified 14 genome-wide significant windows for stroke on 4q33-34.1, of which two replicated in T1D, and a suggestive genomic window on LINC01500, which replicated in T1D. Finally, we identified a suggestively stroke-associated TRPM2-AS promoter (p-value = 5.78 × 10–6) with borderline significant replication in T1D, which we validated with an in vitro cell-based assay. Due to the rarity of the identified genetic variants, future replication of the genomic regions represented here is required with sequencing of individuals with T1D. Nevertheless, we here report the first genome-wide analysis on stroke in individuals with diabetes.
Profiles of objective and subjective cognitive function in Post-COVID Syndrome, COVID-19 recovered, and COVID-19 naïve individuals
Authors: Bland A. R. , Barraclough M. , Trender W. R. , Mehta M. A. , Hellyer P. J. , Hampshire A. , Penner I. K. , Elliott R. , Harenwall S.
Published: June 2024

Post-COVID Syndrome has emerged as a significant public health concern worldwide with increasing evidence to suggest that individuals who have had an acute COVID-19 infection report lingering memory and attention difficulties, even in individuals who have fully recovered and no longer experiencing symptoms of COVID-19. The present study sought to investigate the profile of objective and subjective cognitive difficulties in people who have Post-COVID Syndrome, people who have fully recovered from an acute COVID infection and people who have never had COVID-19. We further sought to explore the extent to which self-reported fatigue and stress are related to subjective and objective cognitive difficulties. 162 participants including 50 people living with Post-COVID Syndrome, 59 people who have had COVID-19 but have fully recovered and 53 people who have never experienced symptoms of COVID-19 and had never tested positive for COVID-19 were recruited from Academic Prolific to complete a series of online questionnaires and neurocognitive tasks. Subjective cognitive function was measured using the Cognitive Failures Questionnaire and objective cognitive function was measured using the Cognitron cognitive test battery. We found that objective and subjective measures of cognitive function were not significantly related, suggesting that self-reports of “brain fog” are not reflecting objectively measured cognitive dysfunction. A MANOVA revealed that subjective cognitive deficits were driven by heightened perceived stress and fatigue and not significantly related to COVID-19 status. Objective cognitive function, however, was significantly related to perceived stress and COVID status whereby we observed significant objective cognitive deficits in people who have been exposed to an acute COVID-19 infection regardless of whether they had Post-COVID Syndrome or had fully recovered, as compared to people who had never had COVID-19. This suggests that an acute infection can have long term effects on cognitive function, even without persistent COVID-19 symptoms. Encouragingly, objective cognitive function was significantly associated with time since initial infection showing that cognitive deficits improved over time for people who had recovered from COVID-19. However, we did not observe the same improvement in individuals with Post-COVID Syndrome and observed that cognitive dysfunction was significantly related to the number of neurological symptoms presently experienced. These results add to the accumulating literature that COVID-19 is associated with significant cognitive difficulties following a COVID-19 infection, which appear to improve over time for those who have recovered from COVID-19 yet persist in people living with Post-COVID Syndrome.
Synthesis and characterization of flame retardant unsaturated polyester-allyloxysilane resin for wood coatings
Authors: Mohammadi Dehcheshmeh Iman , Poursattar Marjani Ahmad , Sadegh Fatemeh , Soltani Mohammad Ebrahim , Safaeirad Mohammad , Frediani Marco
Published: June 2024

Fireproof coatings are the simplest, most efficient, and oldest method for protecting a wide range of flammable products, such as wood. Furthermore, surface ignition is the initial phase, so surface protection is essential to reduce fire propagation. Furthermore, delaying the spread of flames can help to save someone when a fire starts. This project synthesized flame-resistant resin starting from tetraallyloxysilane monomer as a halogen-free monomer, an intrinsic flame retardant co-curing agent. The following step synthesized polyester resin using terephthalic acid as a heat-resistant resin. Unsaturated polyester was used by bulk radical polymerization. FT-IR and 1H-NMR analysis showed the successful synthesis of the desired monomer and polymeric compound. The thermal degradation and flame retardancy of pure unsaturated polyester resin (UPE) and allyloxysilane-unsaturated polyester (AUPE) were investigated by thermogravimetric analysis (TGA/DTG/DSC). The burning test and the thermal stability of the coating layers were evaluated using standard UL 94. Physical properties of resins were evaluated using Heat Deflection Temp tests (HDT) ISO 75-A, ASTM 648, Hardness ASTM D2583, Volumetric shrinkage ASTM 3521, and Water absorption ASTM D570. The results of the tests show the successful synthesis and their flame retardant properties.
Paleoproteomic identification of the species used in fourteenth century gut-skin garments from the archaeological site of Nuulliit, Greenland
Authors: Cucina Annamaria , Schmidt Anne Lisbeth , Di Gianvincenzo Fabiana , Mackie Meaghan , Dove Carla , Jakobsen Aviâja Rosing , Grønnow Bjarne , Appelt Martin , Cappellini Enrico
Published: June 2024

Until recently, the identification of the species of origin for skin and fur materials used in the production of archaeological clothing has been based on the analysis of macro- and microscopic morphological features and on the traditional knowledge of Indigenous groups. This approach, however, is not always applicable due to the deterioration of the archaeological objects. Paleoproteomics was used as an alternative approach to identify the species of origin of fifteen samples of various tissues from approximately 600-year-old garments found in Nuulliit, northern Greenland. Proteomics revealed that a limited group of marine and terrestrial mammals were used for clothing production. The results obtained from the analysis of multiple types of clothing and elements, such as sinew thread and gut skin, suggest that their applications were based on their properties. When conclusive assignment of a sample to a species via proteomics was not possible, the observation by transmitted light microscopy of feather and hair micromorphology, if not affected by diagenesis, was used to improve the identification. The proteomic characterization of animal materials used for clothing production in the Nuulliit archaeological context provides an insight into the practical knowledge and the strategies adopted by the local Indigenous community to exploit natural resources.
U-shaped association between serum calcium and in-hospital mortality in diabetes patients with congestive heart failure: a cohort study
Authors: Zhang Kai , Zhang Tianqi , Lv Qian Yu , Han Yu , Cai Tianyi , Gu Fang ming , Gu Zhao xuan , Zhao Jia Yu , liang Jia Ying , Gao Min , Gao Ya Fang , Hu Rui , Cui Dan , Li Bo , Liu Kexiang
Published: June 2024

Previous studies have reported that the significant association between serum calcium and mortality substantially in patients, especially among those with intensive care unit (ICU). And In diabetes mellitus, congestive heart failure (CHF) is a significant comorbidity. We aim to evaluate the association between serum calcium levels and in-hospital mortality among patients with diabetes and congestive heart failure. The participants in this study were extracted from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database. To scrutinize potential associations between serum calcium levels and in-hospital mortality, a comprehensive analysis encompassing multivariate logistic regression, cubic spline function model, threshold effect analysis, and subgroup analysis was performed. This retrospective cohort study encompassed 7063 patients, among whom the in-hospital mortality stood at 12.2%. In the multivariate logistic regression, adjusted odds ratios (ORs) were contrasted with the reference category Q6 (8.8–9.1 mg/dL) for serum calcium levels and in-hospital mortality. The adjusted ORs for Q1 (≤ 7.7 mg/dL), Q2 (7.7–8 mg/dL), and Q7 (≥ 9.1 mg/dL) were 1.69 (95% CI 1.17–2.44, p = 0.005), 1.62 (95% CI 1.11–2.36, p = 0.013), and 1.57 (95% CI 1.1–2.24, p = 0.012) respectively. The dose–response analysis uncovered a U-shaped relationship between serum calcium levels and in-hospital mortality in diabetic patients with heart failure. Subgroup analyses confirmed result stability notwithstanding the influence of diverse factors. Our investigation revealed a U-shaped correlation between serum calcium levels and in-hospital mortality in diabetes patients with congestive heart failure, pinpointing a significant inflection point at 9.05 mg/dL.
Human mobility description by physical analogy of electric circuit network based on GPS data
Authors: Zhong Zhihua , Takayasu Hideki , Takayasu Misako
Published: June 2024

Human mobility in an urban area is complicated; the origins, destinations, and transportation modes of each person differ. The quantitative description of urban human mobility has recently attracted the attention of researchers, and it highly related to urban science problems. Herein, combined with physics inspiration, we introduce a revised electric circuit model (RECM) in which moving people are regarded as charged particles and analogical concepts of electromagnetism such as human conductivity and human potential enable us to capture the characteristics of urban human mobility. We introduce the unit system, ensure the uniqueness of the calculation result, and reduce the computation cost of the algorithm to 1/10,000 compared with the original ECM, making the model more universal and easier to use. We compared features including human conductivity and potential between different major cities in Japan to show our improvement of the universality and the application range of the model. Furthermore, based on inspiration of physics, we propose a route generation model (RGM) to simulate a human flow pattern that automatically determines suitable routes between a given origin and destination as a source and sink, respectively. These discoveries are expected to lead to new approaches to the solution of urban science problems.
Associations of lifestyle characteristics with circulating immune markers in the general population based on NHANES 1999 to 2014
Authors: Guo Linfen , Huang Yating , He Jing , Li Deng , Li Wei , Xiao Haitao , Xu Xuewen , Zhang Yange , Wang Ru
Published: June 2024

Lifestyles maybe associated with the immune and inflammatory state of human body. We aimed to comprehensively explore the relationship between lifestyles and circulating immune-inflammatory markers in the general population. Data from NHANES 1999–2014 was used. Lifestyle factors included leisure-time physical activity (LTPA), diet quality (Healthy Eating Index-2015, HEI-2015), alcohol consumption, cigarettes smoking, sleep hour and sedentary time. Immune makers included C-reactive protein (CRP), neutrophil–lymphocyte ratio (NLR), systemic immune-inflammation index (SII), platelet–lymphocyte ratio (PLR) and monocyte–lymphocyte ratio (MLR). Generalized linear regression models were used to adjust confounders. Regressions of restricted cubic splines were utilized to evaluate the potentially non-linear relationships between exposures and outcomes. As results, HEI was negatively associated with CRP (P < 0.001), SII (P < 0.001), and NLR (P < 0.001). Cigarettes per day was positively associated with CRP (P < 0.001), SII (P < 0.001), and NLR (P = 0.008). Alcohol consumption was negatively associated with CRP (P < 0.001), but positively associated with PLR (P = 0.012) and MLR (P < 0.001). Physical activity was negatively associated with CRP (P < 0.001), SII (P = 0.005), and NLR (P = 0.002), but positively associated with PLR (P = 0.010). Participants with higher healthy lifestyle score had significantly lower CRP, SII and NLR (all P values < 0.05). Most of the sensitivity analyses found similar results. In conclusion, we found significant associations between lifestyles and immune markers in the general population, which may reflect a systemic inflammatory response to unhealthy lifestyles.
Investigating clinical pharmacokinetics of brivaracetam by using a pharmacokinetic modeling approach
Authors: Qayyum Attia , Zamir Ammara , Rasool Muhammad Fawad , Imran Imran , Ahmad Tanveer , Alqahtani Faleh
Published: June 2024

The development of technology and the processing speed of computing machines have facilitated the evaluation of advanced pharmacokinetic (PK) models, making modeling processes simple and faster. The present model aims to analyze the PK of brivaracetam (BRV) in healthy and diseased populations. A comprehensive literature review was conducted to incorporate the BRV plasma concentration data and its input parameters into PK-Sim software, leading to the creation of intravenous (IV) and oral models for both populations. The developed physiologically based pharmacokinetic (PBPK) model of BRV was then assessed using the visual predictive checks, mean observed/predicted ratios (Robs/pre), and average fold error for PK parameters including the maximum systemic concentration (Cmax), the area under the curve at time 0 to t (AUC0–∞), and drug clearance (CL). The PBPK model of BRV demonstrated that mean Robs/pre ratios of the PK parameters remained within the acceptable limits when assessed against a twofold error margin. Furthermore, model predictions were carried out to assess how AUC0–∞ is affected following the administration of BRV in individuals with varying degrees of liver cirrhosis, ranging from different child–pugh (CP) scores like A, B, and C. Moreover, dose adjustments were recommended by considering the variations in Cmax and CL in various kidney disease stages (mild to severe).
Higher-contrast images are better remembered during naturalistic encoding
Authors: Brook Limor , Kreichman Olga , Masarwa Shaimaa , Gilaie-Dotan Sharon
Published: June 2024

It is unclear whether memory for images of poorer visibility (as low contrast or small size) will be lower due to weak signals elicited in early visual processing stages, or perhaps better since their processing may entail top-down processes (as effort and attention) associated with deeper encoding. We have recently shown that during naturalistic encoding (free viewing without task-related modulations), for image sizes between 3°–24°, bigger images stimulating more visual system processing resources at early processing stages are better remembered. Similar to size, higher contrast leads to higher activity in early visual processing. Therefore, here we hypothesized that during naturalistic encoding, at critical visibility ranges, higher contrast images will lead to higher signal-to-noise ratio and better signal quality flowing downstream and will thus be better remembered. Indeed, we found that during naturalistic encoding higher contrast images were remembered better than lower contrast ones (~ 15% higher accuracy, ~ 1.58 times better) for images at 7.5–60 RMS contrast range. Although image contrast and size modulate early visual processing very differently, our results further substantiate that at poor visibility ranges, during naturalistic non-instructed visual behavior, physical image dimensions (contributing to image visibility) impact image memory.
Combined oral low-dose cyclophosphamide endocrine therapy may improve clinical response among patients with metastatic breast cancer via Tregs in TLSs
Authors: Zhao Yuze , Wang Shuo , Lv Shuzhen , Liu Xiaojun , Li Weiping , Song Yuguang , Rong Dongwen , Zheng Peiming , Huang Hongyan , Zheng Huixia
Published: June 2024

Despite limited research on refractory and/or endocrine therapy failure in elderly metastatic breast cancer (MBC) patients, a prior study showed that low-dose oral cyclophosphamide (CY) can improve the overall survival rate of MBC patients, possibly through the immunoregulation of regulatory T cells (Tregs). We preliminarily investigated the combination of endocrine therapy (ET) with oral low-dose CY as salvage therapy in elderly patients via peripheral blood regulatory T-cell analyses. In addition, we evaluated the associations of tumor tertiary lymphoid structures (TLSs) with therapeutic outcomes. HR+/HER2− advanced breast cancer patients who received low-dose CY combined with ET or ET only from April 2015 to August 2021 were enrolled in this retrospective study. The primary outcome was the clinical control rate (CCR), and the secondary outcome was progression-free survival (PFS). Circulating T lymphocyte subpopulations represented by Tregs were monitored during treatment by flow cytometry methods. TLSs wereconfirmed by hematoxylin–eosin staining of pretreatment specimens, and CD3, CD4, and Foxp3 were detected using Opal multicolor immunofluorescence. A total of 85 patients who received CY + ET and 50 patients who received ET only were enrolled, the percentage of patients who received CCR was 73% (62/85) vs. 70% (45/50), and the objective response rate (ORR) was 28% (24/85) vs. 24% (12/50). No deaths occurred during the study period. The mean PFS time was 13 vs. 11 months (P = 0.03). In the CY + ET group, decreases in CD4+/CD25+/Foxp3+ T cells (P < 0.001) were favorable for both clinical control and prolonged PFS (P < 0.001). Compared with patients without TLSs, those with TLSs were more likely to have better clinical control and PFS (mean time = 6 months), and a greater number of Treg cells during TLS pretreatment correlated with longer PFS (P = 0.043). Oral low-dose CY combined with standard ET exerts immunological effects by decreasing Treg levels to achieve improved clinical responses. Moreover, patients with TLSs might benefit more from such therapy than those without TLSs, and a high Treg cell count in TLSs before treatment predicts better therapeutic efficacy.
Bottom sediments as an indicator of the restoration potential of lakes—a case study of a small, shallow lake under significant tourism pressure
Authors: Kowalczewska-Madura Katarzyna , Dunalska Julita A. , Kutyła Sebastian , Kobus Szymon
Published: June 2024

The study covered a small, shallow lake, intensively used for recreation (sailing, tourist services and port infrastructure). This study aimed to determine the spatial differentiation of bottom sediments and the potential for phosphorus release in five zones, differing mainly in the type of recreation, depth, direct catchment management, shoreline management and macrophyte presence. The results were used to propose protective and restoration measures to improve the water quality of the studied lake. The innovation in the study was the detailed analysis of bottom sediments, which can be a significant source of pollution besides the external load from the catchment and tourist pressure, in the planned management of this ecosystem. Examination of the physicochemical properties of the bottom sediments showed a clear variation in both composition and potential for internal phosphorus loading. The sediments from the profundal zone, where the most boating activity was observed, together with the sediments from the shallow zone where the boats dock (mooring zone), had the highest potential to supply phosphorus to the bottom waters. This fact was demonstrated by the highest total phosphorus (TP) concentrations in sediments (up to 1.32 mgPg−1 DW) and the content of the most mobile fractions (up to 33%). The other zones associated with the marina, fuel zone, tributary and canal were not significant sources of phosphorus to the ecosystem. Based on the above results, a restoration method involving the removal of bottom sediments from the bottom zone was proposed, supported, of course, by protective measures in the catchment (maintaining a buffer zone around the lake and limiting the inflow of pollutants with tributary waters). The proposed measures with sustainable tourist pressure should improve water quality and thus contribute to protecting this valuable natural landscape.
Enhanced control strategy for photovoltaic emulator operating in continuously changing environmental conditions based on shift methodology
Authors: Harrison Ambe , Ullah Safeer , Alombah Njimboh Henry , Bajaj Mohit , Mbasso Wulfran Fendzi , Iqbal Sheeraz , Tuka Milkias Berhanu
Published: June 2024

This article investigates an inventive methodology for precisely and efficiently controlling photovoltaic emulating (PVE) prototypes, which are employed in the assessment of solar systems. A modification to the Shift controller (SC), which is regarded as a leading PVE controller, is proposed. In addition to efficiency and accuracy, the novel controller places a high emphasis on improving transient performance. The novel piecewise linear-logarithmic adaptation utilized by the Modified-Shift controller (M-SC) enables the controller to linearly adapt to the load burden within a specified operating range. At reduced load resistances, the transient sped of the PVE can be increased through the implementation of this scheme. An exceedingly short settling time of the PVE is ensured by a logarithmic modification of the control action beyond the critical point. In order to analyze the M-SC in the context of PVE control, numerical investigations implemented in MATLAB/Simulink (Version: Simulink 10.4, URL:https://in.mathworks.com/products/simulink.html) were utilized. To assess the effectiveness of the suggested PVE, three benchmarking profiles are presented: eight scenarios involving irradiance/PVE load, continuously varying irradiance/temperature, and rapidly changing loads. These profiles include metrics such as settling time, efficiency, Integral of Absolute Error (IAE), and percentage error (epve). As suggested, the M-SC attains an approximate twofold increase in speed over the conventional SC, according to the findings. This is substantiated by an efficiency increase of 2.2%, an expeditiousness enhancement of 5.65%, and an IAE rise of 5.65%. Based on the results of this research, the new M-SC enables the PVE to experience perpetual dynamic operation enhancement, making it highly suitable for evaluating solar systems in ever-changing environments.
Experimental and numerical evaluation of the dust suppression efficiency of an innovative vortex pneumatic fog screen dust control device
Authors: Ren Shuaishuai , Jing Deji , Ge Shaocheng , Chen Yinuo , Meng Xiangxi , Chang Ping
Published: June 2024

To effectively control the dust generated by coal mining operations, a new type of cyclonic pneumatic mist curtain dust control device was developed. Using CFD software, numerical simulations were conducted on the internal airflow velocity field, the exit velocity of the cyclonic pneumatic mist curtain, and the mist droplet particle field of the curtain. Experiments were carried out to measure the spray coverage, droplet size, and the dust control performance of the model device. The results indicate that when the water pump supply pressure is 8 MPa, the fan supply wind speed is 12 m/s, and the nozzle installation angle is 75 degrees, the cyclonic pneumatic mist curtain dust control device model operates under optimal conditions. The effective coverage of the cyclonic mist curtain is 380 × 3300 mm, fully suppressing dust generation on one side of the curtain. An optimal dust removal distance of about 90 cm was determined. After installing the cyclonic pneumatic mist curtain dust control device, the average dust reduction efficiency for respirable dust reached 91.07%, and the overall dust reduction efficiency achieved 93.34%.
Feasibility assessment of a clean and efficient fire extinguishing system for pottery jar liquor warehouses
Authors: Li Xueming , Wan Wei , Zhao Youkai , Bai Gang , Shi Xunxian , Chen Bing , Zhang Yutao
Published: June 2024

Clean fire extinguishing systems applicable to the pottery jar liquor warehouse are in demand. In this study, taking 53vol% liquor as the research subject, fire models of various clean fire extinguishing systems comprising water mist, liquid carbon dioxide (LCO2) and liquid nitrogen (LN2) were established using a fire dynamic simulator to determine their fire extinguishing effect. A feasibility assessment of systems was performed under different fire source types, fire source sizes, and ventilation conditions. The fire extinguishing efficiency was analyzed in terms of the fire extinguishing time, oxygen concentration, and space temperature. The results showed that the success rate of the LCO2 and LN2 fire extinguishing systems was 100%, whereas the success rate of the water mist fire extinguishing system was 95%. In terms of reducing the oxygen concentration at the bottom of the space and the temperature in the space, the LCO2 system exhibited the best performance, followed by the LN2 system, and lastly the water mist. Under different ventilation conditions and fire source types, the LCO2 fire extinguishing system was least affected, whereas the effectiveness of the water mist fire extinguishing system reduced under natural ventilation conditions, and the extinguishing efficiency of the LN2 fire extinguishing system was affected by the fire source type. Overall, the LCO2 system presented more advantages in extinguishing fires in pottery jar liquor warehouses and can provide a new idea for the development and application of clean and efficient fire extinguishing systems.
Metataxonomic analysis of endophytic bacteria of blackberry (Rubus ulmifolius Schott) across tissues and environmental conditions
Authors: Roca-Couso Rocío , Flores-Félix José David , Deb Saptarathi , Giagnoni Lucia , Tondello Alessandra , Stevanato Piergiorgio , Squartini Andrea , García-Fraile Paula , Rivas Raúl
Published: June 2024

(1) Background: Endophytic bacteria represent an important component of plant wellness. They have been widely studied for their involvement in plant development and enhancement of stress tolerance. In this work, the endophytic communities of roots, stems, and leaves of blackberry (Rubus ulmifolius Schott) were studied in three different niches: natural, riverside, and human-impacted niches. (2) Results: The microbiome composition revealed that Sphingomonadaceae was the most abundant family in all samples, accounting for 9.4–45.8%. In contrast, other families seem to be linked to a specific tissue or niche. Families Microbacteriaceae and Hymenobacteraceae increased their presence in stem and leaf samples, while Burkholderiaceae abundance was important in riverside samples. Alpha and beta diversity analyses showed that root samples were the most diverse, and they gathered together in the same cluster, apart from the rest of the samples. (3) Conclusions: The analysis of the microbiome of R. ulmifolius plants revealed that the composition was essentially the same in different niches; the differences were primarily influenced by plant tissue factors with a core genome dominated by Sphingomonadaceae. Additionally, it was observed that R. ulmifolius can select its own microbiome, and this remains constant in all tissues evaluated regardless the niche of sampling.
Exact analytical soliton solutions of the M-fractional Akbota equation
Authors: Awadalla Muath , Taishiyeva Aigul , Myrzakulov Ratbay , Alahmadi Jihan , Zaagan Abdullah A. , Bekir Ahmet
Published: June 2024

In this paper we explore the new analytical soliton solutions of the truncated M-fractional nonlinear

$$(1+1)$$

(
1
+
1
)

-dimensional Akbota equation by applying the

$$\exp _a$$

exp
a

function technique, Sardar sub-equation and generalized kudryashov techniques. Akbota is an integrable equation which is Heisenberg ferromagnetic type equation and have much importance for the analysis of curve as well as surface geometry, in optics and in magnets. The obtained results are in the form of dark, bright, periodic and other soliton solutions. The gained results are verified as well as represented by two-dimensional, three-dimensional and contour graphs. The gained results are newer than the existing results in the literature due to the use of fractional derivative. The obtained results are very helpful in optical fibers, optics, telecommunications and other fields. Hence, the gained solutions are fruitful in the future study for these models. The used techniques provide the different variety of solutions. At the end, the applied techniques are simple, fruitful and reliable to solve the other models in mathematical physics.
Efficacy and safety of antithrombotic therapy for preventing and treating pediatric thromboembolic disease: a systematic review
Authors: Gao Hongjin , Chen Mingyu , Huang Youqi , Liu Huiting , Lin Yuze , Chen Min
Published: June 2024

This review used traditional and network meta-analyses (NMA) to conduct a comprehensive study of antithrombotic therapies in children with thromboembolic disease. We searched the PubMed, Embase, Cochrane Library, Web of Science and ClinicalTrials.gov databases from their inception to 26 February, 2023. And we finally included 16 randomized controlled trials. In the prevention of thromboembolic events (TEs), the use of anticoagulants had a low risk of TEs (relative risk (RR) 0.73, 95% CI 0.56 to 0.94) and a high risk of minor bleeding (RR 1.43, 95% CI 1.09 to 1.86) compared with no anticoagulants. In the treatment of TEs, direct oral anticoagulants (DOACs) were not inferior to standard anticoagulation in terms of efficacy and safety outcomes. In NMA, rivaroxaban and apixaban showed the lowest risk for TEs and major or clinically relevant nonmajor bleeding. According to the overall assessment of efficacy and safety, dabigatran may be the best choice for children with thromboembolic disease. The results of our study will provide references and suggestions for clinical drug selection.
Enzyme-independent role of EZH2 in regulating cell cycle progression via the SKP2-KIP/CIP pathway
Authors: Colon Tania , Kou Ziyue , Choi Byeong Hyeok , Tran Franklin , Zheng Edwin , Dai Wei
Published: June 2024

While EZH2 enzymatic activity is well-known, emerging evidence suggests that EZH2 can exert functions in a methyltransferase-independent manner. In this study, we have uncovered a novel mechanism by which EZH2 positively regulates the expression of SKP2, a critical protein involved in cell cycle progression. We demonstrate that depletion of EZH2 significantly reduces SKP2 protein levels in several cell types, while treatment with EPZ-6438, an EZH2 enzymatic inhibitor, has no effect on SKP2 protein levels. Consistently, EZH2 depletion leads to cell cycle arrest, accompanied by elevated expression of CIP/KIP family proteins, including p21, p27, and p57, whereas EPZ-6438 treatment does not modulate their levels. We also provide evidence that EZH2 knockdown, but not enzymatic inhibition, suppresses SKP2 mRNA expression, underscoring the transcriptional regulation of SKP2 by EZH2 in a methyltransferase-independent manner. Supporting this, analysis of the Cancer Genome Atlas database reveals a close association between EZH2 and SKP2 expression in human malignancies. Moreover, EZH2 depletion but not enzymatic inhibition positively regulates the expression of major epithelial-mesenchymal transition (EMT) regulators, such as ZEB1 and SNAIL1, in transformed cells. Our findings shed light on a novel mechanism by which EZH2 exerts regulatory effects on cell proliferation and differentiation through its methyltransferase-independent function, specifically by modulating SKP2 expression.
Applications of magnesium iodide structure via modified-polynomials
Authors: Ghazwani Haleemah , Jamil Muhammad Kamran , Ahmad Ali , Azeem Muhammad , Koam Ali N. A.
Published: June 2024

A relatively recent approach in molecular graph theory for analyzing chemical networks and structures is called a modified polynomial. It emphasizes the characteristics of molecules through the use of a polynomial-based procedure and presents numerical descriptors in algebraic form. The Quantitative Structure-Property Relationship study makes use of Modified Polynomials (M-Polynomials) as a mathematical tool. M-Polynomials used to create connections between a material’s various properties and its structural characteristics. In this study, we calculated several modified polynomials and gave a polynomial description of the magnesium iodide structure. Particularly, we computed first, second and modified Zagreb indices based M-polynomials. Randić index, and inverse Randić indices based M-polynomials are also computed in this work.
Upregulation of sperm-associated antigen 5 expression in endometrial carcinoma was associated with poor prognosis and immune dysregulation, and promoted cell migration and invasion
Authors: Chen Manru , Wang Dan , Xu Yanyu , Yang Chenggang
Published: June 2024

Sperm-associated antigen 5 (SPAG5) regulates cancer cell invasion and is involved in the progression of many cancers. However, the role of SPAG5 in endometrial carcinoma (EC) is still unknown. The purpose of this study was to explore the role of SPAG5 in EC and its potential molecular mechanism. The UALCAN tool and cBioPortal were used to analyze the expression and alterations of SPAG5 in EC, respectively. OncoLnc was used for survival analysis. We analyzed the effects of SPAG5 on immune cell infiltration and the expression levels of immune checkpoints. We also overexpressed and knocked down SPAG5 in EC cells to explore the effect of SPAG5 regulation on migration, invasion, apoptosis, and the cell cycle of EC cells. We found that SPAG5 was overexpressed and the SPAG5 gene was often mutated in EC. High SPAG5 expression was significantly associated with poor overall survival in patients with EC. SPAG5 also affected the level of immune cell infiltration in the TIME and the expression of immune checkpoints lymphocyte activating 3 (LAG3) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) in patients with EC. It may also be involved in the immunotherapy response in these patients. In vitro experiments showed that SPAG5 promotes cancer cell migration and invasion. In conclusion, this study lays the foundation for further understanding the molecular mechanisms of EC involving SPAG5 and contributes to diagnosing and managing this disease.
miRNA-seq analysis of high glucose induced osteoblasts provides insight into the mechanism underlying diabetic osteoporosis
Authors: Zhang Yang , Li Mengying , Lou Pengqiang , Zhang Minjie , Shou Dan , Tong Peijian
Published: June 2024

The present study aims to explore the etiology of Diabetic osteoporosis (DOP), a chronic complication associated with diabetes mellitus. Specifically, the research seeks to identify potential miRNA biomarkers of DOP and investigated role in regulating osteoblasts. To achieve this, an animal model of DOP was established through the administration of a high-sugar and high-fat diet, and then injection of streptozotocin. Bone microarchitecture and histopathology analysis were analyzed. Rat calvarial osteoblasts (ROBs) were stimulated with high glucose (HG). MiRNA profiles of the stimulated osteoblasts were compared to control osteoblasts using sequencing. Proliferation and mineralization abilities were assessed using MTT assay, alkaline phosphatase, and alizarin red staining. Expression levels of OGN, Runx2, and ALP were determined through qRT-PCR and Western blot. MiRNA-sequencing results revealed increased miRNA-702-5p levels. Luciferase reporter gene was utilized to study the correlation between miR-702-5p and OGN. High glucose impaired cell proliferation and mineralization in vitro by inhibiting OGN, Runx2, and ALP expressions. Interference with miR-702-5p decreased OGN, Runx2, and ALP levels, which were restored by OGN overexpression. Additionally, downregulation of OGN and Runx2 in DOP rat femurs was confirmed. Therefore, the miRNA-702-5p/OGN/Runx2 signaling axis may play a role in DOP, and could be diagnostic biomarker and therapeutic target for not only DOP but also other forms of osteoporosis.
A targeted likelihood estimation comparing cefepime and piperacillin/tazobactam in critically ill patients with community-acquired pneumonia (CAP)
Authors: Serrano-Mayorga Cristian C. , Duque Sara , Ibáñez-Prada Elsa D. , Garcia-Gallo Esteban , Arrieta María P. Rojas , Bastidas Alirio , Rodríguez Alejandro , Martin-Loeches Ignacio , Reyes Luis F.
Published: June 2024

Cefepime and piperacillin/tazobactam are antimicrobials recommended by IDSA/ATS guidelines for the empirical management of patients admitted to the intensive care unit (ICU) with community-acquired pneumonia (CAP). Concerns have been raised about which should be used in clinical practice. This study aims to compare the effect of cefepime and piperacillin/tazobactam in critically ill CAP patients through a targeted maximum likelihood estimation (TMLE). A total of 2026 ICU-admitted patients with CAP were included. Among them, (47%) presented respiratory failure, and (27%) developed septic shock. A total of (68%) received cefepime and (32%) piperacillin/tazobactam-based treatment. After running the TMLE, we found that cefepime and piperacillin/tazobactam-based treatments have comparable 28-day, hospital, and ICU mortality. Additionally, age, PTT, serum potassium and temperature were associated with preferring cefepime over piperacillin/tazobactam (OR 1.14 95% CI [1.01–1.27], p = 0.03), (OR 1.14 95% CI [1.03–1.26], p = 0.009), (OR 1.1 95% CI [1.01–1.22], p = 0.039) and (OR 1.13 95% CI [1.03–1.24], p = 0.014)]. Our study found a similar mortality rate among ICU-admitted CAP patients treated with cefepime and piperacillin/tazobactam. Clinicians may consider factors such as availability and safety profiles when making treatment decisions.
Heat shock protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 °C
Authors: Garriga Ferran , Maside Carolina , Padilla Lorena , Recuero Sandra , Rodríguez-Gil Joan E. , Yeste Marc
Published: June 2024

At present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing for the maintenance of cell lifespan. In this context, the study of proteins that could protect sperm during liquid storage is of high relevance. The 70 kDa Heat Shock Protein (HSP70) is an anti-apoptotic protein that has been reported to be relevant to sperm survival. Thus, we explored the role of HSP70 during prolonged storage of pig semen at 17 °C. Six semen pools were incubated with YM-1 (0, 0.05, 0.1 and 0.2 μM), an HSP70 inhibitor, and stored at 17 °C for 21 days. On days 0, 4, 10, 14 and 21, sperm quality and function were evaluated through flow cytometry and Computer-Assisted Sperm Analysis (CASA), and HSP70 activity and chromatin condensation were also determined. While inhibition of HSP70 increased progressive motility, Ca2+ and Reactive Oxygen Species (ROS) levels, and mitochondrial activity during the first 10 days of storage, it had a detrimental effect on sperm motility after 14 and 21 days. In spite of this, sperm viability was not altered. We can conclude that HSP70 contributes to the liquid storage of pig semen because it keeps mitochondrial activity low, which is needed for the maintenance of sperm function.
Anomalously weak intensity of tropical cyclones striking eastern China over the past two millennia
Authors: Yang Yang , Maselli Vittorio , Zhou Liang , Gao Jianhua , Gao Ya , Wang Ya Ping , Jia Jianjun , Gao Shu
Published: June 2024

Anthropogenic climate warming is predicted to increase the intensity of global tropical cyclones (TCs) on decadal timescales, known as the ‘temperature-TC intensity’ paradigm. However, no proxy is currently available to directly quantify TC intensity in the northwestern Pacific region over centennial to millennial timescales. Here, we reconstruct the intensity of past TCs inferred from event-beds detected in two sedimentary systems in eastern China spanning approximately 1910 to 645 yr BP using an instrumental-calibrated technique, thereby encompassing a sufficiently wide range of temperatures to test the paradigm in the time domain. Intriguingly, our two intensity indices, based on flooding depth and wind speed, provide the initial quantitative evidence that TC intensity in eastern China has been anomalously weak since around 1485 ± 45 yr BP, with a reduction of approximately 30 ± 8% in intensity, despite no concurrent temperature shift. This reduction appears to have been pre-conditioned by a combined influence of a weaker El Niño-Southern Oscillation, a stronger Atlantic Meridional Overturning Circulation, and an increased level of Saharan dust. We suggest that the magnitudes of these factors may have crossed a tipping point and have not reverted to their pre-shift levels since that time, resulting in their impact on TC intensity exceeding that of temperature by triggering changes in the oceanic and atmospheric state within the tropical Pacific region where TCs originate.
Spaceflight induces changes in gene expression profiles linked to insulin and estrogen
Authors: Mathyk Begum Aydogan , Tabetah Marshall , Karim Rashid , Zaksas Victoria , Kim JangKeun , Anu R. I. , Muratani Masafumi , Tasoula Alexia , Singh Ruth Subhash , Chen Yen-Kai , Overbey Eliah , Park Jiwoon , Cope Henry , Fazelinia Hossein , Povero Davide , Borg Joseph , Klotz Remi V. , Yu Min , Young Steven L. , Mason Christopher E. , Szewczyk Nathaniel , St Clair Riley M. , Karouia Fathi , Beheshti Afshin
Published: June 2024

Organismal adaptations to spaceflight have been characterized at the molecular level in model organisms, including Drosophila and C. elegans. Here, we extend molecular work to energy metabolism and sex hormone signaling in mice and humans. We found spaceflight induced changes in insulin and estrogen signaling in rodents and humans. Murine changes were most prominent in the liver, where we observed inhibition of insulin and estrogen receptor signaling with concomitant hepatic insulin resistance and steatosis. Based on the metabolic demand, metabolic pathways mediated by insulin and estrogen vary among muscles, specifically between the soleus and extensor digitorum longus. In humans, spaceflight induced changes in insulin and estrogen related genes and pathways. Pathway analysis demonstrated spaceflight induced changes in insulin resistance, estrogen signaling, stress response, and viral infection. These data strongly suggest the need for further research on the metabolic and reproductive endocrinologic effects of space travel, if we are to become a successful interplanetary species.
A multifunctional chitosan based porous membrane for pH-responsive antibacterial activity and promotion of infected wound healing
Authors: Pu Shan , Zhang Jiale , Shi Chaoting , Hou Xiandeng , Li Ka , Feng Jinhua , Wu Lan
Published: June 2024

A novel CS/hydroxyapatite/ZIF-8 porous membrane has been constructed by in situ loading of ZIF-8 on CS/hydroxyapatite. The membrane shows multiple functions including hemostasis, antibacterial, and promotion of cell proliferation and tissue growth.
High-precision Helicobacter pylori infection diagnosis using a dual-element multimodal gas sensor array
Authors: Wu Jiaying , Xu Shiyuan , Liu Xuemei , Zhao Jingwen , He Zhengfu , Pan Aiwu , Wu Jianmin
Published: June 2024

A non-invasive, label-free and high-precision diagnosis of Helicobacter pylori (H. pylori) infection is conducted by using a dual-element multimodal gas sensor array.
Encapsulation of hydroxycamptothecin within porous and hollow poly(l-lactide-co-ε-caprolactone) microspheres as a floating delivery system for intravesical instillation
Authors: Zhuang Shiya , Jin Xingwei , Cen Lian , Shao Yuan
Published: June 2024

Sustained release of hydroxycamptothecin with anti-cancer effects from porous and hollow floating PLCL microspheres for intravesical instillation.
Iron(ii) spin crossover complexes of tetradentate Schiff-bases: tuning T1/2 by choice of formyl-heterocycle component
Authors: Robb Matthew G. , Chong Shen V. , Brooker Sally
Published: June 2024

Heterocycle choice in four structurally characterised solvent-free [FeII(Lheterocycle)(NCS)2] complexes of easily accessed Schiff base ligands strongly influences solid state spin crossover behaviour.
A stable hybrid catalyst (POM-PPPh3/L/Ni) for the reduction of toxic nitroarene compounds in water
Authors: Aghajani Shima , Mohammadikish Maryam
Published: June 2024

A new heterogeneous nanocatalyst was prepared via the functionalization of a Keggin-type polyoxometalate through strong electrostatic interactions. This nanocatalyst indicated very good efficiency in reduction of nitroarenes under mild reaction conditions and a short reaction time.