bluuebunny/crossref_metadata_2025 huggingface dataset:
Network pharmacology study of Curcuma longa L.: potential target proteins and their functional enrichment analysis
Authors: Kumari Sangeeta , Subramanya Hosahalli S.
Published: October 2020

Objective

This study’s primary goal is unraveling the mechanism of action of bioactives of Curcuma longa L. at the molecular level using protein–protein interaction network.

Results

We used target proteins to create protein–protein interaction network (PPIN) and identified significant node and edge attributes of PPIN. We identified the cluster of proteins in the PPIN, which were used to identify enriched pathways. We identified closeness centrality and jaccard score as most important node and edge attribute of the PPIN respectively. The enriched pathways of various clusters were overlapped suggesting synergistic mechanism of action. The three pathways found to be common among three clusters were Gonadotropin-releasing hormone receptor pathway, Endothelin signaling pathway, and Inflammation mediated by chemokine and cytokine signaling pathway.
Effects of hydroxyapatite-coated nonwoven polyethylene/polypropylene fabric on non-mesodermal lineage-specific differentiation of human adipose-derived stem cells
Authors: Ntege Edward Hosea , Sunami Hiroshi , Denda Junko , Futenma Naoko , Shimizu Yusuke
Published: October 2020

Objective

Compared to other stem cells, the multipotency of human adipose-derived mesenchymal stem cells (ASCs) is limited. Effective approaches that trigger or enhance lineage-specific transdifferentiation are highly envisaged in the improvement of ASCs-based cell therapies. Using Immunofluorescence assays and the secretion of cardiac troponin T (cTnT) protein, we studied the impact of two substrates: Hydroxyapatite (HAp)-coated nonwoven polyethylene (PET)/polypropylene (PP) fabric and glass surfaces, representing 3 dimensional (D) and 2 D environments respectively, on the induction of cardiomyocytes – a non-mesodermal cell type from ASCs for 1–5 weeks.

Results

ASCs were successfully isolated from human adipose tissue under cGMP conditions. Within 1–3 weeks, expression of cTnT in the induced 3D cultures was overall significantly higher (P < 0.021) than that in the induced 2D cultures or controls (P < 0.0009). Remarkably, after 3 weeks of culture, cTnT secretion in the induced 3D cultures gradually declined, nearly reaching levels observed in the 2D cultures. The results show that HAp-coated nonwoven PE/PP fabric could enhance lineage-specific differentiation of ASCs toward cardiac-like cells. However, the fabric might suppress growth of the transformed cells. These preliminary findings encourage further interest in validating the fabric’s potential in improving ASCs transdifferentiation.
An ecological study of the association between environmental indicators and early childhood caries
Authors: Folayan Morenike Oluwatoyin , Tantawi Maha El , Gaffar Balgis , Schroth Robert J. , Catillo Jorge L. , Al-Batayneh Ola B. , Kemoli Arthur , Díaz Aída Carolina Medina , Pavlic Verica , Raswhan Maher , Group for Early Childhood Caries Advocacy
Published: October 2020

Objectives

A prior study described the association between ecosystem vitality, environmental health, and early childhood caries (ECC). The objective of this study was to determine the association between 24 global environmental indicators and ECC in 3–5-year-old children.

Results

In 61 countries, 55.5% of 3–5-year-old children had ECC. Eight factors had a small effect-size association with ECC: percentage of area that is marine-protected (partial eta squared; ƞ2 = 0.03); species habitat index (ƞ2 = 0.06); percentage of tree-cover loss (ƞ2 = 0.03); regional marine trophic index (ƞ2 = 0.03); total carbon dioxide emission intensity (ƞ2 = 0.03); methane emission intensity (ƞ2 = 0.04); nitrous oxide emission intensity (ƞ2 = 0.06); and sulfur dioxide emission intensity (ƞ2 = 0.03). Regression analysis revealed that two of these factors were significantly associated with the prevalence of ECC: methane emission intensity was inversely associated with ECC prevalence (B = − 0.34, 95% CI = − 0.66, − 0.03; p = 0.03), and nitrous oxide had a direct association with ECC prevalence (B = 0.35, 95% CI = 0.04, 0.67; p = 0.03).
A review of optical chemical structure recognition tools
Authors: Rajan Kohulan , Brinkhaus Henning Otto , Zielesny Achim , Steinbeck Christoph
Published: October 2020

Structural information about chemical compounds is typically conveyed as 2D images of molecular structures in scientific documents. Unfortunately, these depictions are not a machine-readable representation of the molecules. With a backlog of decades of chemical literature in printed form not properly represented in open-access databases, there is a high demand for the translation of graphical molecular depictions into machine-readable formats. This translation process is known as Optical Chemical Structure Recognition (OCSR). Today, we are looking back on nearly three decades of development in this demanding research field. Most OCSR methods follow a rule-based approach where the key step of vectorization of the depiction is followed by the interpretation of vectors and nodes as bonds and atoms. Opposed to that, some of the latest approaches are based on deep neural networks (DNN). This review provides an overview of all methods and tools that have been published in the field of OCSR. Additionally, a small benchmark study was performed with the available open-source OCSR tools in order to examine their performance.
Quantitative evaluation of hepatic integrin αvβ3 expression by positron emission tomography imaging using 18F-FPP-RGD2 in rats with non-alcoholic steatohepatitis
Authors: Hiroyama Shuichi , Rokugawa Takemi , Ito Miwa , Iimori Hitoshi , Morita Ippei , Maeda Hiroki , Fujisawa Kae , Matsunaga Keiko , Shimosegawa Eku , Abe Kohji
Published: October 2020

Background

Integrin αvβ3, which are expressed by activated hepatic stellate cells in non-alcoholic steatohepatitis (NASH), play an important role in the fibrosis. Recently, we reported that an RGD peptide positron emission tomography (PET) probe is useful as a predictor of hepatic fibrosis. Kinetic analysis of the RGD PET probe has been performed in tumours, but not in hepatic fibrosis. Therefore, we aimed to quantify hepatic integrin αvβ3 in a model of NASH by kinetic analysis using 18F-FPP-RGD2, an integrin αvβ3 PET probe.

Methods

18F-FPP-RGD2 PET/CT scans were performed in control and NASH rats. Tissue kinetic analyses were performed using a one-tissue, two-compartment (1T2C) and a two-tissue, three-compartment (2T3C) model using an image-derived input function (IDIF) for the left ventricle. We then conducted correlation analysis between standard uptake values (SUVs) or volume of distribution (VT), evaluated using compartment kinetic analysis and integrin αv or β3 protein expression.

Results

Biochemical and histological evaluation confirmed the development of NASH rats. Integrin αvβ3 protein expression and hepatic SUV were higher in NASH- than normal rats. The hepatic activity of 18F-FPP-RGD2 peaked rapidly after administration and then gradually decreased, whereas left ventricular activity rapidly disappeared. The 2T3C model was found to be preferable for 18F-FPP-RGD2 kinetic analysis in the liver. The VT (IDIF) for 18F-FPP-RGD2, calculated using the 2T3C model, was significantly higher in NASH- than normal rats and correlated strongly with hepatic integrin αv and β3 protein expression. The strengths of these correlations were similar to those between SUV60–90 min and hepatic integrin αv or β3 protein expression.

Conclusions

We have demonstrated that the VT (IDIF) of 18F-FPP-RGD2, calculated using kinetic modelling, positively correlates with integrin αv and β3 protein in the liver of NASH rats. These findings suggest that hepatic VT (IDIF) provides a quantitative assessment of integrin αvβ3 protein in liver.
Anomaly detection for electricity consumption in cloud computing: framework, methods, applications, and challenges
Authors: Feng Longji , Xu Shu , Zhang Linghao , Wu Jing , Zhang Jidong , Chu Chengbo , Wang Zhenyu , Shi Haoyang
Published: October 2020

Driven by industrial development and the rising population, the upward trend of electricity consumption is not going to curb. While the electricity suppliers make every endeavor to satisfy the needs of consumers, they are facing the plight of indirect losses caused by technical or non-technical factors. Technical losses are usually induced by short circuits, power outage, or grid failures. The non-technical losses result from humans’ improper behaviors, e.g., electricity burglars. Due to the restrictions of the detection methods, the detection rate in the traditional power grid is lousy. To provide better electricity service for the customers and minimize the losses for the providers, a leap in the power grid is occurring, which is referred to as the smart grid. The smart grid is envisioned to increase the detection accuracy to an acceptable level by utilizing modern technologies, such as cloud computing. With the aim of obtaining achievements of anomaly detection for electricity consumption with cloud computing, we firstly introduce the basic definition of anomaly detection for electricity consumption. Next, we conduct the surveys on the proposed framework of anomaly detection for electricity consumption and propose a new framework with cloud computing. This is followed by centralized and decentralized detection methods. Then, the applications of centralized and decentralized detection methods for the anomaly electricity consumption are listed. Finally, the open challenges of the accuracy of detection and anomaly detection for electricity consumption with edge computing are discussed.
On post quantum estimates of upper bounds involving twice $(p,q)$-differentiable preinvex function
Authors: Awan Muhammad Uzair , Talib Sadia , Noor Muhammad Aslam , Chu Yu-Ming , Noor Khalida Inayat
Published: October 2020

The main objective of this paper is to derive a new post quantum integral identity using twice$(p,q)$(p,q)-differentiable functions. Using this identity as an auxiliary result, we obtain some new post quantum estimates of upper bounds involving twice$(p,q)$(p,q)-differentiable preinvex functions.
Determinants that influence the performance of women entrepreneurs in micro and small enterprises in Ethiopia
Authors: Alene Endalew Terefe
Published: October 2020

Purpose

The purpose of this study was to explore determinants that influence women entrepreneurs’ performance in micro and small enterprises in Gondar city, Northwest Ethiopia, and in turn contribute to entrepreneurship-related literature mainly in developing countries.

Design/methodology/approach

The study employed an explanatory research design with agreement of primary data collection via a cross-sectional survey questionnaire followed by quantitative research approach. The sample of this study was 180 women entrepreneurs and selected using random sampling technique.

Findings

The findings of this study revealed that educational level, previous entrepreneurial experience, access to business training, access to finance, access to business information, government support, land ownership, and tax are significant in explaining women entrepreneurs’ performance in one hand. On the other side, however, age, marital status, access to market, and access to physical infrastructure are found to be insignificant variables in determining women entrepreneurs’ performance.

Research limitations/implications

The study used one time data for determinants like level of education, previous working experience, age, and profitability of the enterprise. Thus, due to frequent change in such variables, the study may not reflect the dynamics of the data, which would have a convincing influence on the conclusion. In addition, the research has only consisted of 180 samples. Moreover, such number may not represent the whole population of the entrepreneurs of Ethiopian MSEs. In future research, it is advisable to expand study factors, use interviews as a research tool, and make a comparison between women and men entrepreneurial performances.

Practical implications

The paper might serve as an input for officials to consider such determinants and encourage an environment that increases women entrepreneurs’ performance. In addition, the study might help women entrepreneurs in addressing the factors affecting performance to take actions towards improving their performance and in turn contribute to job creation, wealth, innovation, and poverty alleviation.

Originality/value

This study contributes to the literature on the determinants of women entrepreneurs’ performance in micro and small enterprises. Specifically, it tests the impact of educational level, age, marital status, previous entrepreneurial experience, access to business training, access to finance, access to business information, access to market, access to physical infrastructure, government support, land ownership, and tax on the performance of women entrepreneurs.
Physicochemical changes during 4 weeks ripening of Camembert cheeses salted with four types of salts
Authors: Bae Hyung Churl , Nam Joong Hyeon , Renchinkhand Gereltuya , Choi Suk-Ho , Nam Myoung Soo
Published: October 2020

The objectives of this study were to compare physicochemical, rheological and sensory characteristics of Camembert cheeses salted with 4 types of salts (refined salt, baked refined salt, sun-dried salt, and Himalayan rock salt) during 4 weeks ripening period. The pH of Camembert cheese was 7.2 in the sun-dried salt, which was the highest than the other three types of salts. The viable lactic acid bacteria (LAB) counts after 4 weeks ripening of the cheeses with 4 types of salts were 1.4 × 108, 1.6 × 108, 1.3 × 108, and 1.3 × 108 CFU/ml, respectively. The water soluble nitrogen level of the cheese salted with Himalayan rock salt was the highest (109.23 µg/g) among those of all the cheeses at 4 weeks ripening. Organic acids in all the cheeses decreased as the ripening period advanced. Protein hydrolysis proceeded faster refined salted cheese than other salted cheeses after 4 weeks ripening. Octadecanoic acid and hexadecanoic acid (volatile fatty acid) which were detected in the cheeses with refined salt and Himalayan rock salt were lower than those with baked refined salt and sun-dried salt. In terms of textural characteristics there were substantially steep decreases in hardness, gumminess, and chewiness after 4 weeks ripening, while springiness and cohesiveness decreased less in all the Camembert cheeses. With respect to sensory properties, the taste score of the cheese with refined salt were significantly higher than those with baked refined salt, sun-dried salt and Himalayan rock salt. These results suggested that the refined salt should be recommended for Camembert cheese making.
The ultimate preoperative C-reactive protein-to-albumin ratio is a prognostic factor for survival after pancreatic cancer resection
Authors: van Wijk Laura , de Klein Guus W. , Kanters Matthijs A. , Patijn Gijs A. , Klaase Joost M.
Published: October 2020

Background

Emerging evidence indicates that an elevated C-reactive protein-to-albumin ratio (CAR) may be associated with a poor prognosis in pancreatic ductal adenocarcinoma (PDAC). Further evidence showing that this ratio has significant prognostic value could contribute to current prediction models and clinical decision-making.

Methods

Data were analysed of consecutive patients who underwent curative pancreatic resection between 2013 and 2018 and were histologically diagnosed with PDAC. We investigated the relation between the ultimate preoperative CAR and overall survival.

Results

A total of 163 patients were analysed. Median overall survival was 18 months (IQR 9–36). Multivariate analysis demonstrated that a higher CAR (HR 1.745, P = 0.004), a higher age (HR 1.062, P < 0.001), male sex (HR 1.977, P = 0.001), poor differentiation grade (HR 2.812, P < 0.001), and positive para-aortic lymph node(s) (HR 4.489, P < 0.001) were associated with a lower overall survival. Furthermore, a CAR ≥ 0.2 was associated with decreased overall survival (16 vs. 26 months, P = 0.003).

Conclusion

We demonstrated that an ultimate preoperative elevated CAR is an independent indicator of decreased overall survival after resection for PDAC. The preoperative CAR may be of additional value to the current prediction models.
A silver nanowire-based flexible pressure sensor to measure the non-nutritive sucking power of neonates
Authors: de Oliveira Jean G. , Muhammad Tausif , Kim Sohee
Published: October 2020

Preterm infants are prone to have higher risks of morbidity, disability and developmental delay compared to term infants. The primitive reflexes, inborn behaviors found in early life development, are shown to be a good tool to assess the integrity of the central nervous system of infants and to predict potential malfunctions. Among these reflexes, the non-nutritive sucking reflex plays an important role in indicating congenital abnormalities in brain development and feeding readiness, especially for premature infants. Conventionally, pediatricians evaluate the oral sucking power qualitatively based on their experiences, by using a gloved finger put inside the infant’s mouth. Thus, more quantitative solutions to assess the sucking power of preterm infants are necessary to support healthcare professionals in their evaluation procedures. Here, we developed a silver nanowire (AgNW)-based flexible pressure sensor to measure the non-nutritive sucking power of infants. The flexible sensor was fabricated using silver nanowires deposited on polydimethylsiloxane (PDMS) in a sandwich-like structure. The sensor based on the principle of strain gauge was attached to a ring-shaped connecting module, and then to a pacifier. The negative sucking pressure exerted by the infant deformed the sensor membrane, causing its electrical resistance to change without any contact between the infant’s mouth and the sensing element. The fabricated sensor was characterized and optimized to achieve both the suitable sensitivity and stability. Thanks to the excellent long-term electro-mechanical stability and high sensitivity, the developed sensor is expected to provide the means to quantitatively assess the non-nutritive sucking of infants, with a portable, low-cost, non-invasive and light-weight solution.
Xenograft for anterior cruciate ligament reconstruction was associated with high graft processing infection
Authors: Van Der Merwe Willem , Lind Martin , Faunø Peter , Van Egmond Kees , Zaffagnini Stefano , Marcacci Maurilio , Cugat Ramon , Verdonk Rene , Ibañez Enrique , Guillen Pedro , Marcheggiani Muccioli Giulio Maria
Published: October 2020

Purpose

To evaluate clinical ad radiological outcomes of anterior cruciate ligament (ACL) reconstruction with an immunochemically modified porcine patellar tendon xenograft controlled against human Achilles tendon allograft at 24-month minimum follow-up.

Methods

66 patients undergoing arthroscopic ACL reconstruction were randomized into 2 groups: 34 allografts and 32 xenografts treated to attenuate the host immune response. Follow-up was 24-month minimum. Anterior knee stability was measured as KT − 1000 side-to-side laxity difference (respect to the contralateral healthy knee). Functional performance was assessed by one-legged hop test. Objective manual pivot-shift test and subjective (IKDC, Tegner and SF-36) outcomes were collected. MRI and standard X-Ray were performed.

Results

61 subjects (32 allograft, 29 xenograft) were evaluated at 12 and 24 months. Six of the subjects in xenograft group (20.6%) got an infection attributed to a water-based pathogen graft contamination in processing.

Intention-to-treat analysis (using the last observation carried forward imputation method) revealed higher KT − 1000 laxity in xenograft group at 24-month follow-up (P = .042). Also pivot-shift was higher in xenograft group at 12-month (P = .015) and 24-month follow-up (P = .038).

Per-protocol analysis (missing/contaminated subjects excluded) did not revealed clinical differences between groups.

Tibial tunnel widening in the allograft group was low, whereas xenograft tunnel widening was within the expected range of 20–35% as reported in the literature.

No immunological reactivity was associated to xenograft group.

Conclusions

High infection rate (20.6%) was reported in xenograft group. Both groups of patients achieved comparable clinical outcomes if missing/contaminated subjects are excluded. Improved harvesting/processing treatments in future studies using xenografts for ACL reconstruction are needed to reduce infection rate, otherwise xenograft should not be used in ACL reconstruction.

Level of evidence

Multicenter and double-blinded Randomized Controlled Clinical Trial, Level I.
Extracellular volume fraction measurement correlates with lymphocyte abundance in thymic epithelial tumors
Authors: Chang Chao-Chun , Lin Chia-Ying , Chu Chang-Yao , Hsiung Yi-Cheng , Chuang Ming-Tsung , Tseng Yau-Lin , Yen Yi-Ting
Published: October 2020

Background

Recent advance in tissue characterization with parametric mapping imaging has the potential to be a novel biomarker for histopathologic correlation with thymic epithelial tumors (TETs). The purpose of our study is to evaluate MRI T1 mapping with the calculation of extracellular volume (ECV) fraction for histologic correlation with thymic epithelial tumor based on lymphocyte abundance.

Methods

A retrospective study including 31 consecutive patients (14 men and 17 women, median age, 56 years; interquartile range, 12 years) with TETs was performed. The T1 values and ECV were assessed by using quantitative MRI mapping techniques. Mann-Whitney U test, Kruskal-Wallis H test, and receiver operating characteristic curve analyses were used to assess discrimination between different types of TETs based on lymphocyte abundance.

Results

Extracellular volume was significantly higher in TETs with sparse lymphocyte, including type A, type B3, and thymic carcinoma, compared with those with abundant lymphocyte, including type B1, B2, and AB thymomas (42.5% vs 26.9%, respectively; p < 0.001). Extracellular volume was significantly higher in thymic carcinoma compared with low grade and high grade thymomas (48.6% vs 31.1% vs 27.6%, respectively; p = 0.002).

Conclusions

T1 mapping with the calculation of extracellular volume (ECV) fraction correlate with the WHO histologic classification of thymic epithelial tumor based on lymphocyte abundance.
Biotic and environmental changes in the Panthalassa Ocean across the Norian (Late Triassic) impact event
Authors: Sato Honami , Takaya Yutaro , Yasukawa Kazutaka , Fujinaga Koichiro , Onoue Tetsuji , Kato Yasuhiro
Published: October 2020

Stratigraphic records of impact ejecta preserved in a pelagic deep-sea setting occur within Upper Triassic successions of the subduction-generated accretionary complexes of central Japan. A significant biotic turnover in radiolarians occurred during the ~ 300 kyr time interval after the impact event, which is characterized by a remarkable reduction in the burial flux of radiolarian silica. However, the nature of the environmental conditions at this time remains unclear. To investigate the environmental changes that triggered a decline in radiolarian burial flux after the impact event, geochemical proxies (major, trace, and rare earth elements) were applied to the middle–upper Norian (Upper Triassic) bedded chert succession of the Mino Belt, central Japan. A progressive environmental deterioration is evident from (1) a post-impact shutdown of burial flux of primary and silica- and apatite-secreting organisms; and (2) a subsequent abrupt increase in chemical weathering associated with a sustained reduction in the burial flux of radiolarian silica. No significant redox changes were observed across the impact event. The continental weathering proxies suggest a transient increase in weathering intensity occurred during the decline of radiolarian burial flux, likely in response to a short-term warm and humid period. Our results delineate a remarkable record of progressive environmental changes in the Panthalassa Ocean after this large impact event.
Successful treatment with positive airway pressure ventilation for tension pneumopericardium after pericardiocentesis in a neonate: a case report
Authors: Tani Makiko , Kanazawa Tomoyuki , Shioji Naohiro , Shimizu Kazuyoshi , Iwasaki Tatsuo , Morimatsu Hiroshi
Published: October 2020

Background

Pneumopericardium in neonates is often associated with respiratory diseases, of which positive pressure ventilation (PPV) is an exacerbating factor. Here, we present a neonate case of pneumopericardium after cardiac surgery which was resolved after applying PPV.

Case presentation

A 28-day-old neonate with left recurrent nerve palsy after aortic reconstruction for interrupted aortic arch developed pericardial effusion. Pericardiocentesis was performed under general anesthesia, and a drainage tube was left in the pericardium. After extubation, stridor gradually exacerbated, following hemodynamic deterioration. A chest X-ray demonstrated pneumopericardium. Upper airway stenosis due to recurrent nerve palsy developed excessive negative pleural pressure, and air was drawn into pericardium via the insertion site of the drainage tube. After tracheal intubation and applying PPV, the pneumopericardium improved.

Conclusion

PPV does not always exacerbate pneumopericardium. In a patient with pericardial-atmosphere communication, increased inspiration effort can cause pneumopericardium, and PPV is a therapeutic option to alleviate the pneumopericardium.
Prevalence of peripheral arterial diseases in patients with large artery ischemic stroke and its prognostic value
Authors: Farag Sherien , Elbalkimy Mahmoud , Elbassiouny Ahmed , George John , Fathy Mai
Published: October 2020

Background

Peripheral artery disease (PAD) can be an important predictor of comorbid cerebrovascular disease (CVD). However, it is not sufficiently investigated or considered in the management and prevention of stroke.

Objectives

To study the prevalence of peripheral arterial disease in large artery ischemic stroke patients and its impact on prognosis.

Methods

This is a prospective cohort study. A total of 100 patients with large artery ischemic stroke were enrolled with assessment for signs of PAD; duplex on both lower limb arteries and measurement of ankle-brachial index (ABI), carotid duplex, and/or C.T angiography brain and neck and/or conventional angiography; functional assessment by NIHSS at days 0, 7, and 30; and follow-up for recurrence or death after 6 months.

Results

Peripheral arterial disease was found in 42% of cases. Fifty percent of patients with lower extremity arterial disease were asymptomatic. Fifty percent had significant carotid stenosis (stenosis > 50%), and 48% of patients with significant carotid stenosis had peripheral arterial disease. During our study, 8 cases had a recurrence of large artery ischemic stroke, and 5 cases (62.5%) had PAD. The recurrence rate of stroke was the highest in the asymptomatic PAD group with 19%.

Conclusion

The prevalence of PAD in large artery ischemic stroke patients is high. Asymptomatic PAD has a risk potential for large artery ischemic stroke recurrence and mortality more than symptomatic PAD and non-PAD patients.
‘They don’t quite understand the importance of what we’re doing today’: the young people’s climate strikes as subaltern activism
Authors: Bowman Benjamin
Published: October 2020

Background

Youth-led movements like #FridaysforFuture and the school strikes for climate (henceforth referred to as the climate strikes) are leading calls for action on climate change worldwide. This paper reports on a thematic analysis of protest signs, and interviews with young climate strikers, at a climate strike in Manchester, UK, in 2019.

Results

This paper explores the ways in which dominant, adult-centred frameworks for conceptualizing young people’s environmental activism tend to obscure the complexities of the climate strike movement. In contrast, this study examines the complex political activism of climate strikers as a ‘subaltern group’, who take political action in a wider context of intersecting categories of oppression and marginalization – including youth as a category of marginalization – and in the historical context of environmental racism, the enduring legacies of colonialism, and global inequality during contemporary capitalism.

Conclusions

The article develops a theoretical model for future research, based on a model of two constraining frames that limit analysis of the climate strikes in particular and young people’s environmental activism in general. This paper contributes to a step change in methods for the study of this remarkable movement in a global context.
Baseline Framingham risk score does not predict future ECG-derived QRS duration over an average of 3.3 years
Authors: Stone Elijah , Zhou Yuling , Jelinek Herbert , Mclachlan Craig S.
Published: October 2020

Background

Prolonged electrocardiogram (ECG) QRS duration has been associated with increased cardiovascular risk. It is unclear whether the main predictor of cardiovascular risk, the Framingham risk score also predicts short-term changes in ECG QRS duration. Our aim is to determine whether baseline Framingham risk score is associated with baseline or changes in QRS duration.

Methods

A retrospective cross-sectional analysis was performed using observational data obtained from two hundred two participants. Framingham risk score was calculated using an online risk calculator. QRS duration was obtained using a 10 s trace from a Welch Allyn PC-based 12-lead ECG system.

Results

Average follow-up duration was 3.3 ± 1.1 years. Mean QRS change was 1.8 ± 11.4 ms. Specifically, among two hundred two participants, there are 104 subjects with a greater QRS duration at follow-up, while 98 subjects had the same or a shorter follow-up QRS duration. Baseline Framingham risk score did not significantly predict an increase in QRSd with an odds ratio of 1.04 (P = 0.230). Regression analysis of QRS duration at baseline and Framingham risk at baseline had a weak association (R2 = 0.020; P = 0.043). The Framingham risk score at follow-up was likewise has a weak association with follow-up QRS duration (R2 = 0.045; P = 0.002).

Conclusions

Our results do not demonstrate a statistically significant association between Framingham risk parameters and future QRS duration changes over longitudinal time. QRS duration had variable changes between baseline and follow-up. This might suggest that a longer period of follow-up is required to document more stable increases in QRS duration associated with ventricular pathology. A larger population study is needed to confirm our observations.
Unexpected effects of ivermectin and selamectin on inducible CreER activity in mice
Authors: Kropp Peter A. , Rushing Gabrielle V. , Brockman Asa A. , Yu Erin N. Z. , Ihrie Rebecca A. , Gannon Maureen
Published: October 2020

Background

Anti-parasitics are frequently used in research animal facilities to treat a multitude of common infections, with pinworms and fur mites being amongst the most common. Ivermectin and selamectin are common oral and topical treatments for these infections, respectively. Although commonly thought to be innocuous to both the research animals and any transgenic elements that the animals may carry, evidence exists that ivermectin is capable of activating the recombinase activity of at least one CreER. The goal of the current study was to determine if there was an effect of either anti-parasitic agent on the activity of CreER proteins in transgenic mice.

Case presentation

We analyzed the offspring of transgenic mice exposed to either ivermectin or selamectin during pregnancy and nursing. Through analysis of reporter genes co-expressed with multiple, independently generated transgenic CreER drivers, we report here that ivermectin and selamectin both alter recombinase activity and thus may have unintended consequences on gene inactivation studies in mice.

Conclusions

Although the mechanisms by which ivermectin and selamectin affect CreER activity in the offspring of treated dams remain unclear, the implications are important nonetheless. Treatment of pregnant transgenic mice with these anti-parasitics has the potential to alter transgene activity in the offspring. Special considerations should be made when planning treatment of transgenic mice with either of these pharmacologics.
Ultrasonic pitch and catch technique for non-destructive testing of reinforced concrete slabs
Authors: Zatar Wael A. , Nguyen Hai D. , Nghiem Hien M.
Published: October 2020

This study aims at evaluating reinforced concrete (RC) bridge elements using ultrasonic pitch and catch (UPC) non-destructive testing (NDT) technique. A validation reinforced concrete slab with two embedded layers of rebars and artificial defects (voids, honeycombs, and debondings) was designed and tested. A commercial UPC NDT device (hereafter called “UPC device”), which is based on the ultrasonic shear-wave test method using dry-point-contact transmitting and receiving transducers in a “pitch-catch” configuration, was used to map internal defects of the validation RC slab. The recorded data from the UPC device was analyzed using a modified synthetic aperture focusing technique (SAFT). A software was developed to reconstruct 2-D images of the RC slab cross-sections using novel signal filtering and processing techniques. The results revealed that the 2-D image reconstructed from the developed software accurately exhibited locations and horizontal dimensions of the steel rebars, voids, and debondings. In addition, the developed software was capable to provide much higher resolution and sharper images of the anomalies inside the RC slab compared to the UPC device’s proprietary imaging software.
Etiological profile of upper airway obstruction in infants
Authors: Abo Elmagd Essam , El Hawary Bahaa , Hassan Megahed M. , Kassem Hanem , El Tahan Abd El Rahman
Published: October 2020

Background

Respiratory distress is recognized as any sign of breathing difficulty in infants. Some congenital anomalies present immediately with airway distress, while others are asymptomatic or discovered later in infancy or childhood. Our objectives are to detect different laryngeal causes of respiratory distress in infants and to measure the relative risk of some variables like age, sex, family history, and other congenital anomalies for developing laryngeal causes of respiratory distress. This observational cross-sectional study was carried out during the period from June 2017 to December 2018 at Children’s University Hospital. The study included 80 infants who presented with respiratory distress and admitted to the hospital. All patients subjected to a detailed history from their parents and full general and ENT examinations. X-ray, MSCT neck or direct laryngoscope were carried out in selected cases.

Results

As regard age, 58% of cases were between 1–6 months, 28% of cases between 6 months and 1 year, and 14% between 1–2 years. Laryngeal causes of respiratory distress among infants were distributed as follow: 70% laryngomalacia, 18% subglottic stenosis, 8% laryngeal web, and 4% of cases were caused by vocal fold paralysis (VFP). The laryngeal causes of respiratory distress did not reveal any statistically significant difference among different age groups or between both genders (P = 0.257, 0.286; respectively). Also, there was no statistically significant difference between infants with positive family history and those without as regard the laryngeal causes of respiratory distress (P = .378).

Conclusion

The majority of respiratory distress cases (58%) were between 1–6 months. Bivariant analysis of variables age, sex, family history, and other congenital anomalies showed that they were not potent risk factors for developing laryngomalacia, subglottic stenosis, laryngeal web, and vocal fold paralysis. Laryngomalacia represented the commonest cause of respiratory distress (70%) followed by subglottic stenosis (18%), then laryngeal web (8%), and finally the vocal fold paralysis represented the least percentage (4%). Apart from laryngomalacia, the percentages of other causes seem to vary according to sample size and geographic area of study. So, being familiar with the common causes of respiratory distress in a given region is very essential in order not to miss a potentially life-threatening diagnosis.
Effect of fungal, oomycete and nematode interactions on apple root development in replant soil
Authors: Tilston Emma L. , Deakin Gregory , Bennett Julie , Passey Thomas , Harrison Nicola , Fernández Felicidad , Xu Xiangming
Published: October 2020

Background

Apple replant disease (ARD) is a phenomenon associated with poor tree establishment at sites where the same, or a closely-related species, has grown for at least 1–2 years. No single organism has been identified as the universal causal agent, but there is increasing evidence that multiple soil-borne plant pathogenic fungi and oomycetes form an ARD disease complex. Root damage caused by root lesion nematodes has also been implicated in facilitating the entry of pathogens into root tissues resulting in the development of severe ARD.

Methods

We used a reductionist approach to determine effects of one or more members of the ARD complex on ARD in a number of selected rootstock genotypes with contrasting characteristics. Through a 15-month pot-based experiment in which semi-selective biocides were applied to soil from a replant orchard, we investigated (1) the nature of the interactions (i.e. antagonistic, additive or synergistic) between different groups of soil biota and ARD severity, and (2) whether rootstock characteristics modify ARD severity.

Results

There might be competitive interactions between oomycetes and fungal pathogens in infecting apple roots and hence subsequent ARD development. Controlling all three ARD components (oomycetes, fungi, and nematodes) led to the best root development. However, these effects on root development were not manifested in the above-ground tree development 15 months after treatment. Specific soil biocide treatments against fungi and oomycetes led to large changes in soil microbial communities whereas the nematicide treatment led to least changes. In spite of the observed ARD, comparing rhizosphere microbial sequences among treatments failed to reveal candidate pathogens for ARD.

Conclusions

Candidate ARD oomycetes and fungal pathogens are likely to engage in competitive interactions among themselves in infecting apple roots. Although soil amendments affected soil microbiota, such effects appear to be very unpredictable.
Dissociative identity disorder: out of the shadows at last?
Authors: Reinders Antje A. T. S. , Veltman Dick J.
Published: October 2020

Dissociative identity disorder (DID) is a severely debilitating disorder. Despite recognition in the current and past versions of the DSM, DID remains a controversial psychiatric disorder, which hampers its diagnosis and treatment. Neurobiological evidence regarding the aetiology of DID supports clinical observations that it is a severe form of post-traumatic stress disorder.
Randomized Phase III KEYNOTE-181 Study of Pembrolizumab Versus Chemotherapy in Advanced Esophageal Cancer
Authors: Kojima Takashi , Shah Manish A. , Muro Kei , Francois Eric , Adenis Antoine , Hsu Chih-Hung , Doi Toshihiko , Moriwaki Toshikazu , Kim Sung-Bae , Lee Se-Hoon , Bennouna Jaafar , Kato Ken , Shen Lin , Enzinger Peter , Qin Shu-Kui , Ferreira Paula , Chen Jia , Girotto Gustavo , de la Fouchardiere Christelle , Senellart Helene , Al-Rajabi Raed , Lordick Florian , Wang Ruixue , Suryawanshi Shailaja , Bhagia Pooja , Kang S. Peter , Metges Jean-Philippe ,   on behalf of the KEYNOTE-181 Investigators
Published: October 2020

PURPOSE

Patients with advanced esophageal cancer have a poor prognosis and limited treatment options after first-line chemotherapy.

PATIENTS AND METHODS

In this open-label, phase III study, we randomly assigned (1:1) 628 patients with advanced/metastatic squamous cell carcinoma or adenocarcinoma of the esophagus, that progressed after one prior therapy, to pembrolizumab 200 mg every 3 weeks for up to 2 years or chemotherapy (investigator’s choice of paclitaxel, docetaxel, or irinotecan). Primary end points were overall survival (OS) in patients with programmed death ligand-1 (PD-L1) combined positive score (CPS) ≥ 10, in patients with squamous cell carcinoma, and in all patients (one-sided α 0.9%, 0.8%, and 0.8%, respectively).

RESULTS

At final analysis, conducted 16 months after the last patient was randomly assigned, OS was prolonged with pembrolizumab versus chemotherapy for patients with CPS ≥ 10 (median, 9.3 v 6.7 months; hazard ratio [HR], 0.69 [95% CI, 0.52 to 0.93]; P = .0074). Estimated 12-month OS rate was 43% (95% CI, 33.5% to 52.1%) with pembrolizumab versus 20% (95% CI, 13.5% to 28.3%) with chemotherapy. Median OS was 8.2 months versus 7.1 months (HR, 0.78 [95% CI, 0.63 to 0.96]; P = .0095) in patients with squamous cell carcinoma and 7.1 months versus 7.1 months (HR, 0.89 [95% CI, 0.75 to 1.05]; P = .0560) in all patients. Grade 3-5 treatment-related adverse events occurred in 18.2% of patients with pembrolizumab versus 40.9% in those who underwent chemotherapy.

CONCLUSION

Pembrolizumab prolonged OS versus chemotherapy as second-line therapy for advanced esophageal cancer in patients with PD-L1 CPS ≥ 10, with fewer treatment-related adverse events.
Disparities at the Intersection of Race and Ethnicity: Examining Trends and Outcomes in Hispanic Women With Breast Cancer
Authors: Champion Cosette D. , Thomas Samantha M. , Plichta Jennifer K. , Parrilla Castellar Edgardo , Rosenberger Laura H. , Greenup Rachel A. , Hyslop Terry , Hwang E. Shelley , Fayanju Oluwadamilola M.
Published: October 2020

PURPOSE:

We sought to examine tumor subtype, stage at diagnosis, time to surgery (TTS), and overall survival (OS) among Hispanic patients of different races and among Hispanic and non-Hispanic (NH) women of the same race.

METHODS:

Women 18 years of age or older who had been diagnosed with stage 0-IV breast cancer and who had undergone lumpectomy or mastectomy were identified in the National Cancer Database (2004-2014). Tumor subtype and stage at diagnosis were compared by race/ethnicity. Multivariable linear regression and Cox proportional hazards modeling were used to estimate associations between race/ethnicity and adjusted TTS and OS, respectively.

RESULTS:

A total of 44,374 Hispanic (American Indian [AI]: 79 [0.2%]; Black: 1,011 [2.3%]; White: 41,126 [92.7%]; Other: 2,158 [4.9%]) and 858,634 NH women (AI: 2,319 [0.3%]; Black: 97,206 [11.3%]; White: 727,270 [84.7%]; Other: 31,839 [3.7%]) were included. Hispanic Black women had lower rates of triple-negative disease (16.2%) than did NH Black women (23.5%) but higher rates than did Hispanic White women (13.9%; P < .001). Hispanic White women had higher rates of node-positive disease (23.2%) versus NH White women (14.4%) but slightly lower rates than Hispanic (24.6%) and NH Black women (24.5%; P < .001). Hispanic White women had longer TTS versus NH White women regardless of treatment sequence (adjusted means: adjuvant chemotherapy, 42.71 v 38.60 days; neoadjuvant chemotherapy, 208.55 v 201.14 days; both P < .001), but there were no significant racial differences in TTS among Hispanic patients. After adjustment, Hispanic White women (hazard ratio, 0.77 [95% CI, 0.74 to 0.81]) and Black women (hazard ratio, 0.75 [95% CI, 0.58 to 0.96]) had improved OS versus NH White women (reference) and Black women (hazard ratio, 1.15 [95% CI, 1.12 to 1.18]; all P < .05).

CONCLUSION:

Hispanic women had improved OS versus NH women, but racial differences in tumor subtype and nodal stage among Hispanic women highlight the importance of disaggregating racial/ethnic data in breast cancer research.
Bronchovenous Fistula During Adult Cardiac Surgery: A Case Report
Authors: Saita Kosuke , Kariya Taro , Ezaka Mariko , Nakao Tatsuya , Kin Nobuhide
Published: October 2020

Bronchovenous fistula (BVF) associated with adult cardiac surgery is a rarely reported life-threatening condition. We present a 75-year-old woman who developed a BVF during cardiac surgery. Dense adhesion in the pleural and pericardial cavities was noted. Restrictive pulmonary pathology required high airway pressure. Transesophageal echocardiography and hemoglobin measurement were helpful for the timely diagnosis of BVF, which was controlled by transection of the right upper pulmonary vein where a vent catheter had been inserted. Injuries around the cannulated site presumably initiated the BVF, which was worsened by high-pressure ventilation. Therefore, cannulation site might be a risk factor for BVF.
Utility of Motor Evoked Potential in Identification and Treatment of Suboptimal Positioning in Pediatric Craniovertebral Junction Abnormalities: A Case Report
Authors: Krishnakumar Mathangi , Ramesh Venkatapura , Goyal Amit , Srinivas Dwarakanath
Published: October 2020

Atlantoaxial dislocation (AAD) poses a significant challenge in terms of positioning and surgical fixation as there is increased risk of cord compression and ischemia. Intraoperative neuromonitoring (IONM) provides a useful tool to identify impending position-related dysfunction. Here we demonstrate the utility of using transcranial motor evoked potential (Tc-MEP) to identify and treat suboptimal positioning in a 13-year-old patient with AAD and Arnold-Chiari malformation.
Hippo signaling promotes Ets21c-dependent apical cell extrusion in the Drosophila wing disc
Authors: Ai Xianlong , Wang Dan , Zhang Junzheng , Shen Jie
Published: October 2020

Cell extrusion is a crucial regulator of epithelial tissue development and homeostasis. Epithelial cells undergoing apoptosis, bearing pathological mutations, and possessing developmental defects are actively extruded toward elimination. However, the molecular mechanisms of Drosophila epithelial cell extrusion are not fully understood. Here, we report that activation of the conserved Hippo (Hpo) signaling pathway induces both apical and basal cell extrusion in the Drosophila wing disc epithelia. We show that canonical Yorki targets Diap1, and that dMyc and Cyclin E are not required for either apical or basal cell extrusion induced by activation of this pathway. Another target gene, bantam, is only involved in basal cell extrusion, suggesting novel Hpo-regulated apical cell extrusion mechanisms. Using RNA-Seq analysis, we found that JNK signaling is activated in the extruding cells. We provide genetic evidence that JNK signaling activation is both sufficient and necessary for Hpo-regulated cell extrusion. Furthermore, we demonstrate that the ETS-domain transcription factor Ets21c, an ortholog of proto-oncogenes FLI1 and ERG, acts downstream of JNK signaling to mediate apical cell extrusion. Our findings reveal a novel molecular link between Hpo signaling and cell extrusion.