bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Climate Change on the Moisture Performance of Tallwood Building Envelope
Authors: Defo Maurice , Lacasse Michael
Published: January 2021

The objective of this study was to assess the potential effects of climate change on the moisture performance and durability of massive timber walls on the basis of results derived from hygrothermal simulations. One-dimensional simulations were run using DELPHIN 5.9.4 for 31 consecutive years of the 15 realizations of the modeled historical (1986–2016) and future (2062–2092) climates of five cities located across Canada. For all cities, water penetration in the wall assembly was assumed to be 1% wind-driven rain, and the air changes per hour in the drainage cavity was assumed to be 10. The mold growth index on the outer layer of the cross-laminated timber panel was used to compare the moisture performance for the historical and future periods. The simulation results showed that the risk of mold growth would increase in all the cities considered. However, the relative change varied from city to city. In the cities of Ottawa, Calgary and Winnipeg, the relative change in the mold growth index was higher than in the cities of Vancouver and St. John’s. For Vancouver and St. John’s, and under the assumptions used for these simulations, the risk was already higher under the historical period. This means that the mass timber walls in these two cities could not withstand a water penetration rate of 1% wind-driven rain, as used in the simulations, with a drainage cavity of 19 mm and an air changes per hour value of 10. Additional wall designs will be explored in respect to the moisture performance, and the results of these studies will be reported in a future publication.
Nonlinear Neutral Delay Differential Equations of Fourth-Order: Oscillation of Solutions
Authors: Agarwal Ravi P. , Bazighifan Omar , Ragusa Maria Alessandra
Published: January 2021

The objective of this paper is to study oscillation of fourth-order neutral differential equation. By using Riccati substitution and comparison technique, new oscillation conditions are obtained which insure that all solutions of the studied equation are oscillatory. Our results complement some known results for neutral differential equations. An illustrative example is included.
A 560 GHz Sub-Harmonic Mixer Using Half-Global Design Method
Authors: Zhang Bo , Zhang Yong , Pan Liucheng , Li Yu , Cui Jianhang , Xu Ruimin , Yan Bo
Published: January 2021

In this paper, a 560 GHz terahertz sub-harmonic mixer using a new half-global design method is reported. This method combines the advantages of the subdivision design method and the global design method, and greatly enhances the abilities of the optimization of matching variables while retaining the portability of the unit circuit. When the local oscillator (LO) frequency was fixed with 3 mW power at 280 GHz, average up-conversion double sideband (DSB) conversion loss of 8 dB with intermediate frequency (IF) power of −5 dBm was achieved.
Energy Flexibility as Additional Energy Source in Multi-Energy Systems with District Cooling
Authors: Mugnini Alice , Coccia Gianluca , Polonara Fabio , Arteconi Alessia
Published: January 2021

The integration of multi-energy systems to meet the energy demand of buildings represents one of the most promising solutions for improving the energy performance of the sector. The energy flexibility provided by the building is paramount to allowing optimal management of the different available resources. The objective of this work is to highlight the effectiveness of exploiting building energy flexibility provided by thermostatically controlled loads (TCLs) in order to manage multi-energy systems (MES) through model predictive control (MPC), such that energy flexibility can be regarded as an additional energy source in MESs. Considering the growing demand for space cooling, a case study in which the MPC is used to satisfy the cooling demand of a reference building is tested. The multi-energy sources include electricity from the power grid and photovoltaic modules (both of which are used to feed a variable-load heat pump), and a district cooling network. To evaluate the varying contributions of energy flexibility in resource management, different objective functions—namely, the minimization of the withdrawal of energy from the grid, of the total energy cost and of the total primary energy consumption—are tested in the MPC. The results highlight that using energy flexibility as an additional energy source makes it possible to achieve improvements in the energy performance of an MES building based on the objective function implemented, i.e., a reduction of 53% for the use of electricity taken from the grid, a 43% cost reduction, and a 17% primary energy reduction. This paper also reflects on the impact that the individual optimization of a building with a multi-energy system could have on other users sharing the same energy sources.
A Comparative Analysis of Energy Consumption by Conventional and Anchor Based Dynamic Positioning of Ship
Authors: Łebkowski Andrzej , Wnorowski Jakub
Published: January 2021

One of the requirements for ships equipped with dynamic positioning system is the ability to maintain a given position in various hydrometeorological conditions. At the same time, efforts at reducing electricity consumption are made in order to reduce operating costs and emissions of exhaust gases, such as sulfur oxides and greenhouse gases such as carbon dioxide (CO2). For this purpose, the ship designer at the design stage must predict both how much energy the ship will theoretically use during operation and how the expenditure can be reduced. The publication presents a comparison of energy consumption with two different approaches to ship positioning: the use of classic dynamic positioning utilizing a set of thrusters and by using a set of anchors. In order to determine the energy consumption during positioning, the matrix method was used, on the basis of which the analysis of the ability to hold the position of the ship (capability plot) was performed, in accordance with the recommendations of the classification society DNV GL. Thanks to this analysis, it was possible to find such a distribution of thrust vectors on propulsors that the ship would not lose its set position under the hydrometeorological conditions specified in the analysis. As a result of comparing the two positioning systems, it turned out that using anchor-based positioning uses 24% less energy than positioning based on a set of thrusters, which translates into 24% less CO2 emissions into the atmosphere.
Reduced-Order-VSM-Based Frequency Controller for Wind Turbines
Authors: Lu Liang , Saborío-Romano Oscar , Cutululis Nicolaos A.
Published: January 2021

Frequency support capability is becoming an important requirement for wind turbines, as wind power is increasingly integrated into power systems. In this paper, a frequency controller is implemented and validated. Such a controller allows wind turbines to help regulate the system frequency automatically and includes virtual inertia to help limit the rate of change of frequency. Compared with other methods, the controller achieves satisfactory frequency support capability with considerable simplicity. The controller is added to the grid-side converter controls, together with cascaded inner loops, which enables wind turbines to operate in grid-forming mode with overcurrent protection. The influence of the controller parameters on the frequency response is investigated.
Recovery of Bioactive Compounds from Pomegranate (Punica granatum L.) Peel Using Pressurized Liquid Extraction
Authors: García Paula , Fredes Carolina , Cea Inés , Lozano-Sánchez Jesús , Leyva-Jiménez Francisco Javier , Robert Paz , Vergara Cristina , Jimenez Paula
Published: January 2021

Pressurized liquid extraction (PLE) is a clean and environmentally friendly alternative for the recovery of bioactive compounds from fruit by-products. Herein we focused on PLE for the extraction of bioactive compounds from pomegranate peel using a combination of pressurized water and ethanol. The main aim was to determine the optimal PLE conditions, i.e., ethanol percentage and process temperature, to obtain a pomegranate peel extract (PPE) with maximum total phenolic content (TPC), punicalagin content, and antimicrobial activity (AMA). The experimental design was conducted using a central composite design with axial points. Response surface methodology was applied to optimize the response variables using the desirability function. Multiple response optimization indicated a process temperature of 200 °C and ethanol of 77% as optimal conditions. The TPC and the punicalagin content of PPE-PLE obtained under optimal conditions were 164.3 ± 10.7 mg GAE/g DW and 17 ± 3.6 mg/g DW, respectively. Our findings support the efficacy of PLE on TPC recovery but not in punicalagin recovery. The AMA against S. aureus was 14 mm. The efficacy of PPE-PLE in food applications must continue to be studied in order to achieve adequate information on its potential for developing new food additives.
Impact of a Combination of UV-C Irradiation and Peracetic Acid Spray Treatment on Brochothrix thermosphacta and Yersinia enterocolitica Contaminated Pork
Authors: Koller Valerie , Seinige Diana , Saathoff Julia , Kehrenberg Corinna , Krischek Carsten
Published: January 2021

Efficient ways of decontamination are needed to minimize the risk of infections with Yersinia (Y.) enterocolitica, which causes gastrointestinal diseases in humans, and to reduce the numbers of Brochothrix (B.) thermosphacta to extend the shelf-life of meat. While many studies have focused on a single treatment of peracetic acid (PAA) or UV-C-irradiation, there are no studies about a combined treatment on meat. Therefore, in the present study, pork was inoculated with either Y. enterocolitica or B. thermosphacta, and was treated with a combination of 2040 mJ/cm2 UV-C irradiation followed by a 2000 ppm PAA spray treatment (30 s). Samples were packed under modified atmosphere and stored for 1, 7, or 14 days. The samples were examined for Y. enterocolitica and B. thermosphacta content, chemical and sensory effects, and meat quality parameters. For Y. enterocolitica, a significant reduction of up to 2.16 log10 cfu/cm2 meat and for B. thermosphacta, up to 2.37 log10 cfu/cm2 meat was seen on day 14 after UV-C/PAA treatment compared to the untreated controls.
Obesity Prevention within the Early Childhood Education and Care Setting: A Systematic Review of Dietary Behavior and Physical Activity Policies and Guidelines in High Income Countries
Authors: Jackson Jacklyn Kay , Jones Jannah , Nguyen Hanh , Davies Isabella , Lum Melanie , Grady Alice , Yoong Sze Lin
Published: January 2021

As a strategy for early childhood obesity prevention, a variety of dietary behavior and physical activity policies and guidelines published by leading health agencies and early childhood education and care (ECEC) licensing and accreditation bodies exist. Given the potential diversity in recommendations from these policies, this narrative review sought to synthesize, appraise and describe the various policies and guidelines made by organizational and professional bodies to highlight consistent recommendations and identify opportunities to strengthen such policies. An electronic bibliographic search of seven online databases and grey literature sources was undertaken. Records were included if they were policies or guidelines with specific recommendations addressing dietary behavior and/or physical activity practice implementation within the ECEC setting; included children aged >12 months and <6 years and were developed for high income countries. Recommended dietary behavior and physical activity policies and practices were synthesized into broad themes using the Analysis Grid for Environments Linked to Obesity framework, and the quality of included guidelines appraised. Our search identified 38 eligible publications mostly from the US and Australia. Identified guidelines were largely consistent in their recommendation and frequently addressed the physical and sociocultural environment and were well-aligned with research evidence. Broader consideration of policy and economic environments may be needed to increase the impact of such policies and guidelines within the ECEC setting.
A Qualitative Study of the Feasibility and Acceptability of Implementing ‘Sit-To-Stand’ Desks in Vocational Education and Training
Authors: Kirschner Mara , Golsteijn Rianne H.J. , Sijben Sanne M. , Singh Amika S. , Savelberg Hans H.C.M. , de Groot Renate H.M.
Published: January 2021

While it has been shown that interrupting a person’s sedentary behaviour has the potential to improve cognitive, physical and mental health, a large part of time that students spend in school is sedentary. As research has shown that approximately 80% of vocational education and training (VET) students have an unhealthy sedentary lifestyle, implementing “sit-to-stand” (StS) desks could interrupt sedentary behaviour and promote healthier behaviour. Therefore, the acceptability and feasibility of using such desks in the VET setting should be investigated. Using semi-structured focus group interviews analysed via deductive content analysis, the opinions of 33 students for the following topics were assessed: (1) usage of the standing option of the desks (2) reasons for standing in class (3) experienced effect of standing behind the desk, and (4) fostering future StS desks usage. Although VET students are aware of the potential benefits of using StS desks, they need to be actively stimulated and motivated by teachers to use them. In addition, time is needed to get into the habit of standing. Thus, for successful implementation of StS desks in the VET setting, all stakeholders (i.e., students, teachers, schoolboards) should be actively involved in stimulating the healthy behaviour of VET students.
Anxiety in Frontline and Non-Frontline Healthcare Providers in Kelantan, Malaysia
Authors: Mohd Noor Norhayati , Che Yusof Ruhana , Yacob Mohd Azman
Published: January 2021

In response to the coronavirus disease 2019 (COVID-19) pandemic, healthcare providers are exposed to psychological and mental health implications, including vicarious traumatization, anxiety, and depression. Gradual increases in the number of COVID-19 cases meant they were inadequately protected from contamination due to a shortage of protective equipment, excessive workloads, emotional exhaustion and frustration. These circumstances affect their work performance in delivering health services. This study aims to compare the levels of anxiety in frontline and non-frontline healthcare providers during the COVID-19 pandemic. This study applied a comparative cross-sectional design between May and July 2020 at the Hospital Raja Perempuan Zainab II. Convenient sampling was applied in the selection of eligible participants. The case report form contained two self-administered questionnaires, namely, The Hospital Anxiety and Depression Scale and Medical Outcome Study Social Support Survey. Descriptive analysis, analysis of variance, and analysis of covariance were conducted using SPSS version 26. The number of participants recruited was 306, including 160 healthcare providers in the frontline group and 146 in the non-frontline group. The non-frontline healthcare providers reported a significantly higher anxiety mean score of 1.7 than the frontline providers after adjusting for gender, duration of employment, and social support. It indicates that non-frontline healthcare providers require psychological support similar to that of frontline healthcare providers during the COVID-19 pandemic.
Local-Indigenous Autonomy and Community Streetscape Enhancement: Learnings from Māori and Te Ara Mua—Future Streets Project
Authors: Raerino Kimiora , Macmillan Alex , Field Adrian , Hoskins Rau
Published: January 2021

In settler countries, attention is now extending to the wellbeing benefits of recognising and promoting the Indigenous cultural identity of neighbourhoods as a contributing factor to more equitable and healthier communities. Re-indigenisation efforts to (re)implement cultural factors into urban design can be challenging and ineffective without the leadership and collaboration of local-Indigenous peoples. Undertaken in Aotearoa New Zealand, Te Ara Mua — Future Street project, demonstrated that co-design has critical potential in the reclamation of Indigenous autonomy, increased local-Indigenous presence and revitalisation of cultural identity. Employing a Kaupapa Māori (Māori-centred) research approach, we focused on the workings and perspectives of mana whenua (local-Indigenous peoples) and community stakeholder engagement in Te Ara Mua. An Indigenous theoretical framework, Te Pae Mahutonga, was utilised in the data analysis to explore perspectives of Indigenous collective agency, empowerment, and wellbeing. Our research demonstrates that developing capacity amongst Indigenous communities is integral for effective engagement and that the realisation of autonomy in urban design projects has broader implications for Indigenous sovereignty, spatial justice and health equity. Significantly, we argue that future community enhancement strategies must include not only re-designing and re-imagining initiatives, but also re-indigenising.
Prevalence, Associated Factors and Psychological Determinants of Obesity among Adults in Selangor, Malaysia
Authors: Mohd-Sidik Sherina , Lekhraj Rampal , Foo Chai Nien
Published: January 2021

Background: The pervasiveness of obesity is a growing concern in the world. This study aims to determine the prevalence of obesity among a segment of the Malaysian population, as well as investigate associated factors and psychological determinants of obesity. Methods: A cross-sectional study design was carried out in Selangor, Malaysia. A total of 1380 Malaysian adults (≥18 years old) participated in a structured and validated questionnaire survey. TANITA body scale and SECA 206 body meter were used to measure the respondents’ weight and height, from which measurements of their body mass index (BMI) were calculated. Results: The overall prevalence of obesity (BMI ≥ 30 kg/m2) among adults in Selangor, Malaysia, was 18.6%. Factors significantly associated with increased risk of obesity were: being female (OR = 1.61, 95% CI [1.20–2.17]), aged between 30 to 39 years old (OR = 1.40, 95% CI [1.04–1.88]), being Indian (OR = 1.55, 95% CI [1.13–2.12]), married (OR = 1.37, 95% CI [1.03–1.83]), and having only primary school education (OR = 1.80, 95% CI [1.17–2.78] or secondary school education (OR = 1.37, 95% CI [1.04–1.81]). In the multiple linear regression analysis (stepwise method), perceived stress (B = −0.107, p = 0.041), suicidal ideation (B = −2.423, p = 0.003), and quality of life in the physical health domain (B = −0.350, p = 0.003) inversely and significantly contributed to BMI among males. Among females, stressful life events contributed positively to BMI (B = 0.711, p < 0.001, whereas quality of life in the psychological domain had a negative effect (B = −0.478, p < 0.001) in this respect. Conclusion: There is an urgent need to integrate psychological approaches to enhance the effectiveness of obesity prevention strategies and weight-loss programs.
Comparison of Diagnosed Depression and Self-Reported Depression Symptom as a Risk Factor of Periodontitis: Analysis of 2016–2018 Korean National Health and Nutrition Examination Survey Data
Authors: Kim Seon-Rye , Nam Seoul-Hee
Published: January 2021

Depression causes damage to the immune defense mechanism, and it can worsen periodontitis due to the accumulation of periodontitis pathogens. This study was conducted in order to explore the association of diagnosed depression and self-reported depression symptom with periodontitis by using the Korean National Health and Nutrition Examination Survey 7th (KNHANES VII) data. A total of 12,689 participants aged over 19 received a periodontal examination among the 24,269 participants of KNHANES VII. Diagnosed depression and self-reported depression symptom were the two terms used for depression. Periodontitis was defined as the presence of teeth with periodontal pockets of 4 mm or deeper. The age, sex, marital status, education, region, basic livelihood protection, private health insurance, type of housing, health insurance coverage, household income, sleeping hours, subjective health condition, stress perception, drinking status, obesity, and current smoking status of the participants were examined. Chi-square tests and two-tailed analyses were used. The association of depression and periodontitis was tested by using logistic regression models adjusted for socio-demographic and behavioral variables. Diagnosed depression was associated with periodontitis, as the odds ratio of diagnosed depression for periodontitis was 1.772 (95% confidence interval = 1.328–2.364). However, the association between self-reported depression symptom and periodontitis was not statistically significant. This study revealed that diagnosed depression, not self-reported depression symptom, could be a risk factor for periodontitis. Therefore, it is necessary to take a closer look into diagnosed depression in order to manage and prevent periodontitis.
Diagnosis of Neonatal Late-Onset Infection in Very Preterm Infant: Inter-Observer Agreement and International Classifications
Authors: Bury Gaelle , Leroux Stéphanie , Leon Borrego Cristhyne , Gras Leguen Christèle , Mitanchez Delphine , Gascoin Geraldine , Thollot Aurore , Roué Jean Michel , Carrault Guy , Pladys Patrick , Beuchée Alain
Published: January 2021

Background: The definition of late-onset bacterial sepsis (LOS) in very preterm infants is not unified. The objective was to assess the concordance of LOS diagnosis between experts in neonatal infection and international classifications and to evaluate the potential impact on heart rate variability and rate of “bronchopulmonary dysplasia or death”. Methods: A retrospective (2017–2020) multicenter study including hospitalized infants born before 31 weeks of gestation with intention to treat at least 5-days with antibiotics was performed. LOS was classified as “certain or probable” or “doubtful” independently by five experts and according to four international classifications with concordance assessed by Fleiss’s kappa test. Results: LOS was suspected at seven days (IQR: 5–11) of life in 48 infants. Following expert classification, 36 of them (75%) were considered as “certain or probable” (kappa = 0.41). Following international classification, this number varied from 13 to 46 (kappa = −0.08). Using the expert classification, “bronchopulmonary dysplasia or death” occurred less frequently in the doubtful group (25% vs. 78%, p < 0.001). Differences existed in HRV changes between the two groups. Conclusion: The definition of LOS is not consensual with a low international and moderate inter-observer agreement. This affects the evaluation of associated organ dysfunction and prognosis.
Estimating the Impacts of Proximity to Public Transportation on Residential Property Values: An Empirical Analysis for Hartford and Stamford Areas, Connecticut
Authors: Zhang Bo , Li Weidong , Lownes Nicholas , Zhang Chuanrong
Published: January 2021

Public transit infrastructure may increase residential property values by improving accessibility and reducing commute expenses in urban areas. Prior studies have investigated the impacts of the proximity to public transportation on property values and obtained mixed conclusions. Many of these studies were focused on one transit mode for a single city. In this study, a hedonic pricing model is constructed to investigate the impacts of commuter rail/Bus Rapid Transit (BRT) and bus lines separately in two different areas: the Stamford area (Stamford–Darien–New Canaan) and the Hartford area (Hartford–West Hartford–East Hartford), Connecticut. Comparison of the results from Ordinary Least Square and Geographically Weighted Regression (GWR) indicates that estimation accuracy can be improved by considering local variation. Results from GWR show that impacts of proximity to bus and rail/BRT on property values vary spatially in the Hartford area. Negative impacts of bus stops are found in downtown Hartford and positive impacts in the west and east sides of Hartford. Impacts from rail/BRT are relatively minor compared with bus lines, partly due to the relatively recent launching of the BRT and Hartford rail line. In contrast, most properties in the Stamford area show appreciation towards rail service and depreciation to bus service. This study reveals the roles of different public transit systems in affecting residential property values. It also provides empirical evidence for future transit-oriented development in this region for uplifting the real estate market.
Green Synthesis of Selenium and Tellurium Nanoparticles: Current Trends, Biological Properties and Biomedical Applications
Authors: Zambonino Marjorie C. , Quizhpe Ernesto Mateo , Jaramillo Francisco E. , Rahman Ashiqur , Santiago Vispo Nelson , Jeffryes Clayton , Dahoumane Si Amar
Published: January 2021

The synthesis and assembly of nanoparticles using green technology has been an excellent option in nanotechnology because they are easy to implement, cost-efficient, eco-friendly, risk-free, and amenable to scaling up. They also do not require sophisticated equipment nor well-trained professionals. Bionanotechnology involves various biological systems as suitable nanofactories, including biomolecules, bacteria, fungi, yeasts, and plants. Biologically inspired nanomaterial fabrication approaches have shown great potential to interconnect microbial or plant extract biotechnology and nanotechnology. The present article extensively reviews the eco-friendly production of metalloid nanoparticles, namely made of selenium (SeNPs) and tellurium (TeNPs), using various microorganisms, such as bacteria and fungi, and plants’ extracts. It also discusses the methodologies followed by materials scientists and highlights the impact of the experimental sets on the outcomes and shed light on the underlying mechanisms. Moreover, it features the unique properties displayed by these biogenic nanoparticles for a large range of emerging applications in medicine, agriculture, bioengineering, and bioremediation.
Can SARS-CoV-2 Virus Use Multiple Receptors to Enter Host Cells?
Authors: Gadanec Laura Kate , McSweeney Kristen Renee , Qaradakhi Tawar , Ali Benazir , Zulli Anthony , Apostolopoulos Vasso
Published: January 2021

The occurrence of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), responsible for coronavirus disease 2019 (COVD-19), represents a catastrophic threat to global health. Protruding from the viral surface is a densely glycosylated spike (S) protein, which engages angiotensin-converting enzyme 2 (ACE2) to mediate host cell entry. However, studies have reported viral susceptibility in intra- and extrapulmonary immune and non-immune cells lacking ACE2, suggesting that the S protein may exploit additional receptors for infection. Studies have demonstrated interactions between S protein and innate immune system, including C-lectin type receptors (CLR), toll-like receptors (TLR) and neuropilin-1 (NRP1), and the non-immune receptor glucose regulated protein 78 (GRP78). Recognition of carbohydrate moieties clustered on the surface of the S protein may drive receptor-dependent internalization, accentuate severe immunopathological inflammation, and allow for systemic spread of infection, independent of ACE2. Furthermore, targeting TLRs, CLRs, and other receptors (Ezrin and dipeptidyl peptidase-4) that do not directly engage SARS-CoV-2 S protein, but may contribute to augmented anti-viral immunity and viral clearance, may represent therapeutic targets against COVID-19.
Reticular Basement Membrane Thickness Is Associated with Growth- and Fibrosis-Promoting Airway Transcriptome Profile-Study in Asthma Patients
Authors: Bazan-Socha Stanislawa , Buregwa-Czuma Sylwia , Jakiela Bogdan , Zareba Lech , Zawlik Izabela , Myszka Aleksander , Soja Jerzy , Okon Krzysztof , Zarychta Jacek , Kozlik Paweł , Dziedzina Sylwia , Padjas Agnieszka , Wojcik Krzysztof , Kepski Michal , Bazan Jan G.
Published: January 2021

Airway remodeling in asthma is characterized by reticular basement membrane (RBM) thickening, likely related to epithelial structural and functional changes. Gene expression profiling of the airway epithelium might identify genes involved in bronchial structural alterations. We analyzed bronchial wall geometry (computed tomography (CT)), RBM thickness (histology), and the bronchial epithelium transcriptome profile (gene expression array) in moderate to severe persistent (n = 21) vs. no persistent (n = 19) airflow limitation asthmatics. RBM thickness was similar in the two studied subgroups. Among the genes associated with increased RBM thickness, the most essential were those engaged in cell activation, proliferation, and growth (e.g., CDK20, TACC2, ORC5, and NEK5) and inhibiting apoptosis (e.g., higher mRNA expression of RFN34, BIRC3, NAA16, and lower of RNF13, MRPL37, CACNA1G). Additionally, RBM thickness correlated with the expression of genes encoding extracellular matrix (ECM) components (LAMA3, USH2A), involved in ECM remodeling (LTBP1), neovascularization (FGD5, HPRT1), nerve functioning (TPH1, PCDHGC4), oxidative stress adaptation (RIT1, HSP90AB1), epigenetic modifications (OLMALINC, DNMT3A), and the innate immune response (STAP1, OAS2). Cluster analysis revealed that genes linked with RBM thickness were also related to thicker bronchial walls in CT. Our study suggests that the pro-fibrotic profile in the airway epithelial cell transcriptome is associated with a thicker RBM, and thus, may contribute to asthma airway remodeling.
A Mitochondrial Polymorphism Alters Immune Cell Metabolism and Protects Mice from Skin Inflammation
Authors: Schilf Paul , Künstner Axel , Olbrich Michael , Waschina Silvio , Fuchs Beate , Galuska Christina E. , Braun Anne , Neuschütz Kerstin , Seutter Malte , Bieber Katja , Hellberg Lars , Sina Christian , Laskay Tamás , Rupp Jan , Ludwig Ralf J. , Zillikens Detlef , Busch Hauke , Sadik Christian D. , Hirose Misa , Ibrahim Saleh M.
Published: January 2021

Several genetic variants in the mitochondrial genome (mtDNA), including ancient polymorphisms, are associated with chronic inflammatory conditions, but investigating the functional consequences of such mtDNA polymorphisms in humans is challenging due to the influence of many other polymorphisms in both mtDNA and the nuclear genome (nDNA). Here, using the conplastic mouse strain B6-mtFVB, we show that in mice, a maternally inherited natural mutation (m.7778G > T) in the mitochondrially encoded gene ATP synthase 8 (mt-Atp8) of complex V impacts on the cellular metabolic profile and effector functions of CD4+ T cells and induces mild changes in oxidative phosphorylation (OXPHOS) complex activities. These changes culminated in significantly lower disease susceptibility in two models of inflammatory skin disease. Our findings provide experimental evidence that a natural variation in mtDNA influences chronic inflammatory conditions through alterations in cellular metabolism and the systemic metabolic profile without causing major dysfunction in the OXPHOS system.
Iron Therapy in Chronic Kidney Disease: Days of Future Past
Authors: Lee Kuo-Hua , Ho Yang , Tarng Der-Cherng
Published: January 2021

Anemia affects millions of patients with chronic kidney disease (CKD) and prompt iron supplementation can lead to reductions in the required dose of erythropoiesis-stimulating agents, thereby reducing medical costs. Oral and intravenous (IV) traditional iron preparations are considered far from ideal, primarily due to gastrointestinal intolerability and the potential risk of infusion reactions, respectively. Fortunately, the emergence of novel iron replacement therapies has engendered a paradigm shift in the treatment of iron deficiency anemia in patients with CKD. For example, oral ferric citrate is an efficacious and safe phosphate binder that increases iron stores to maintain hemoglobin levels. Additional benefits include reductions in fibroblast growth factor 23 levels and the activation of 1,25 dihydroxyvitamin D. The new-generation IV iron preparations ferumoxytol, iron isomaltoside 1000, and ferric carboxymaltose are characterized by a reduced risk of infusion reactions and are clinically well tolerated as a rapid high-dose infusion. In patients undergoing hemodialysis (HD), ferric pyrophosphate citrate (FPC) administered through dialysate enables the replacement of ongoing uremic and HD-related iron loss. FPC transports iron directly to transferrin, bypassing the reticuloendothelial system and avoiding iron sequestration. Moreover, this paper summarizes recent advancements of hypoxia-inducible factor prolyl hydroxylase inhibitors and future perspectives in renal anemia management.
Influence of Acetylcholine Esterase Inhibitors and Memantine, Clinically Approved for Alzheimer’s Dementia Treatment, on Intestinal Properties of the Mouse
Authors: Nguyen Vu Thu Thuy , Sallbach Jason , dos Santos Guilherme Malena , Endres Kristina
Published: January 2021

Four drugs are currently approved for the treatment of Alzheimer’s disease (AD) by the FDA. Three of these drugs—donepezil, rivastigmine, and galantamine—belong to the class of acetylcholine esterase inhibitors. Memantine, a NMDA receptor antagonist, represents the fourth and a combination of donepezil and memantine the fifth treatment option. Recently, the gut and its habitants, its microbiome, came into focus of AD research and added another important factor to therapeutic considerations. While the first data provide evidence that AD patients might carry an altered microbiome, the influence of administered drugs on gut properties and commensals have been largely ignored so far. However, the occurrence of digestive side effects with these drugs and the knowledge that cholinergic transmission is crucial for several gut functions enforces the question if, and how, this medication influences the gastrointestinal system and its microbial stocking. Here, we investigated aspects such as microbial viability, colonic propulsion, and properties of enteric neurons, affected by assumed intestinal concentration of the four drugs using the mouse as a model organism. All ex vivo administered drugs revealed no direct effect on fecal bacteria viability and only a high dosage of memantine resulted in reduced biofilm formation of E. coli. Memantine was additionally the only compound that elevated calcium influx in enteric neurons, while all acetylcholine esterase inhibitors significantly reduced esterase activity in colonic tissue specimen and prolonged propulsion time. Both, acetylcholine esterase inhibitors and memantine, had no effect on general viability and neurite outgrowth of enteric neurons. In sum, our findings indicate that all AD symptomatic drugs have the potential to affect distinct intestinal functions and with this—directly or indirectly—microbial commensals.
A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach
Authors: Fei Zhongjie , Wei Rongbin , Cheng Chu , Xiao Pengfeng
Published: January 2021

The COVID-19 pandemic caused by the SARS-CoV-2 virus, which first emerged in December 2019, represents an ongoing global public health emergency. Here, we developed an improved and highly sensitive approach to SARS-CoV-2 detection via coupling bioluminescence in real-time (BART) and reverse-transcriptase loop-mediated amplification (RT-LAMP) protocols (RT-LAMP-BART) and was also compatible with a digital LAMP system (Rainsuit), which did not allow for real-time quantification but did, nonetheless, facilitate absolute quantification with a comparable detection limit of 104 copies/mL. Through improving RNA availability in samples to ensure the target RNA present in reaction, we additionally developed a simulated digital RT-LAMP approach using this same principle to enlarge the overall reaction volume and to achieve real-time detection with a limit of detection of 10 copies/mL, and with further improvements in the overall dynamic range of this assay system being achieved through additional optimization.
Body Mass Index and Disease Activity in Chronic Inflammatory Rheumatic Diseases: Results of the Cardiovascular in Rheumatology (Carma) Project
Authors: Valero-Jaimes Jesús A. , López-González Ruth , Martín-Martínez María A. , García-Gómez Carmen , Sánchez-Alonso Fernando , Sánchez-Costa Jesús T. , González-Juanatey Carlos , Revuelta-Evrad Eva , Díaz-Torné César , Fernández-Espartero Cruz , Pérez-García Carolina , Torrente-Segarra Vicenç , Sánchez-Nievas Ginés , Pérez-Sandoval Trinidad , Font-Ugalde Pilar , García-Vivar María L. , Aurrecoechea Elena , Maiz-Alonso Olga , Valls-García Ramón , Miranda-Filloy José A. , Llorca Javier , Castañeda Santos , Gonzalez-Gay Miguel A.
Published: January 2021

Objective: Since obesity has been associated with a higher inflammatory burden and worse response to therapy in patients with chronic inflammatory rheumatic diseases (CIRD), we aimed to confirm the potential association between body mass index (BMI) and disease activity in a large series of patients with CIRDs included in the Spanish CARdiovascular in rheuMAtology (CARMA) registry. Methods: Baseline data analysis of patients included from the CARMA project, a 10-year prospective study of patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS), and psoriatic arthritis (PsA) attending outpatient rheumatology clinics from 67 Spanish hospitals. Obesity was defined when BMI (kg/m2) was >30 according to the WHO criteria. Scores used to evaluate disease activity were Disease Activity Score of 28 joints (DAS28) in RA, Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) in AS, and modified DAS for PsA. Results: Data from 2234 patients (775 RA, 738 AS, and 721 PsA) were assessed. The mean ± SD BMI at the baseline visit were: 26.9 ± 4.8 in RA, 27.4 ± 4.4 in AS, and 28.2 ± 4.7 in PsA. A positive association between BMI and disease activity in patients with RA (β = 0.029; 95%CI (0.01–0.05); p = 0.007) and PsA (β = 0.036; 95%CI (0.015–0.058); p = 0.001) but not in those with AS (β = 0.001; 95%CI (−0.03–0.03); p = 0.926) was found. Disease activity was associated with female sex and rheumatoid factor in RA and with Psoriasis Area Severity Index and enthesitis in PsA. Conclusions: BMI is associated with disease activity in RA and PsA, but not in AS. Given that obesity is a potentially modifiable factor, adequate control of body weight can improve the outcome of patients with CIRD and, therefore, weight control should be included in the management strategy of these patients.
Phytocannabinoids—A Green Approach toward Non-Alcoholic Fatty Liver Disease Treatment
Authors: Berk Klaudia , Bzdega Wiktor , Konstantynowicz-Nowicka Karolina , Charytoniuk Tomasz , Zywno Hubert , Chabowski Adrian
Published: January 2021

Non-alcoholic fatty liver disease (NAFLD) is the most frequent chronic liver disease in adults in developed countries, with a global prevalence as high as one billion. The pathogenesis of NAFLD is a multifactorial and multi-step process. Nowadays, a growing body of research suggests the considerable role of the endocannabinoid system (ECS) as a complex cell-signaling system in NAFLD development. Although increased endocannabinoid tone in the liver highly contributes to NAFLD development, the complex effects and impacts of plant-derived cannabinoids in the aspect of NAFLD pathophysiology are yet not fully understood, and effective medications are still in demand. In our review, we present the latest reports describing the role of ECS in NAFLD, focusing primarily on two types of cannabinoid receptors. Moreover, we sum up the recent literature on the clinical use of natural cannabinoids in NAFLD treatment. This review is useful for understanding the importance of ECS in NAFLD development, and it also provides the basis for more extensive clinical phytocannabinoids testing in patients suffering from NAFLD.
Introduction to Macroscopic Optimal Design in the Mechanics of Composite Materials and Structures
Authors: Muc Aleksander
Published: January 2021

The main goal of building composite materials and structures is to provide appropriate a priori controlled physico-chemical properties. For this purpose, a strengthening is introduced that can bear loads higher than those borne by isotropic materials, improve creep resistance, etc. Composite materials can be designed in a different fashion to meet specific properties requirements.Nevertheless, it is necessary to be careful about the orientation, placement and sizes of different types of reinforcement. These issues should be solved by optimization, which, however, requires the construction of appropriate models. In the present paper we intend to discuss formulations of kinematic and constitutive relations and the possible application of homogenization methods. Then, 2D relations for multilayered composite plates and cylindrical shells are derived with the use of the Euler–Lagrange equations, through the application of the symbolic package Mathematica. The introduced form of the First-Ply-Failure criteria demonstrates the non-uniqueness in solutions and complications in searching for the global macroscopic optimal solutions. The information presented to readers is enriched by adding selected review papers, surveys and monographs in the area of composite structures.
The Evolution of Arrears among US Households 1995–2013
Authors: Grant Charles
Published: January 2021

This paper looks at arrears among US households between 1995 and 2013. It uses household data from the Survey of Consumer Finances (SCF) where arrears occur when a household reports it “sometimes got behind or missed a payment”. The key contribution is that it decomposes the change in arrears into a behavioural part and a compositional part. Older poorer households increased arrears between 1995 and 2001 (this reversed in 2004). Younger middle-income households increased arrears in 2004. Following bankruptcy reform, wealthier households under 50 reduced their arrears between 2004 and 2007. During the sub-prime recession, everyone except younger low income households increased their arrears. The decomposition exercise shows that most of the changes over time are attributed to changes in arrears once the loan is given and not to the change in the composition of the pool of borrowers.
Synthesis and Application of Cellulose-Polyethyleneimine Composites and Nanocomposites: A Concise Review
Authors: Riva Laura , Fiorati Andrea , Punta Carlo
Published: January 2021

Cellulose/polyethyleneimine composites have increasingly attracted the attention of scientific community, devoted to the design and development of new synthetic strategies and materials for different application fields. In this review, after introducing the main characteristics of the two polymeric components, we provide in the second section a critical overview on the main protocols for the synthesis of these composites, considering both the several cellulose sources and forms, and the different cross-linkers and cross-linking procedures developed for this purpose, outlining advantages and limits for the reported approaches. The last section analyses the principal results obtained in different application fields. A wide discussion is dedicated to the principal use of cellulose/polyethyleneimine composites as sorbents for water remediation from heavy metal ions and organic contaminants. Subsequently, we introduce the literature describing the use of these composites, functionalized appropriately, where necessary, as drug delivery systems, sensors, and heterogeneous catalysts for organic reactions. Finally, after a brief description of other random applications, we furnish a personal analysis of actual limits and potentialities for these systems.
Effect of the Cross-Linking Density on the Swelling and Rheological Behavior of Ester-Bridged β-Cyclodextrin Nanosponges
Authors: Hoti Gjylije , Caldera Fabrizio , Cecone Claudio , Rubin Pedrazzo Alberto , Anceschi Anastasia , Appleton Silvia Lucia , Khazaei Monfared Yousef , Trotta Francesco
Published: January 2021

The cross-linking density influences the physicochemical properties of cyclodextrin-based nanosponges (CD-NSs). Although the effect of the cross-linker type and content on the NSs performance has been investigated, a detailed study of the cross-linking density has never been performed. In this contribution, nine ester-bridged NSs based on β-cyclodextrin (β-CD) and different quantities of pyromellitic dianhydride (PMDA), used as a cross-linking agent in stoichiometric proportions of 2, 3, 4, 5, 6, 7, 8, 9, and 10 moles of PMDA for each mole of CD, were synthesized and characterized in terms of swelling and rheological properties. The results, from the swelling experiments, exploiting Flory–Rehner theory, and rheology, strongly showed a cross-linker content-dependent behavior. The study of cross-linking density allowed to shed light on the efficiency of the synthesis reaction methods. Overall, our study demonstrates that by varying the amount of cross-linking agent, the cross-linked structure of the NSs matrix can be controlled effectively. As PMDA βCD-NSs have emerged over the years as a highly versatile class of materials with potential applications in various fields, this study represents the first step towards a full understanding of the correlation between their structure and properties, which is a key requirement to effectively tune their synthesis reaction in view of any specific future application or industrial scale-up.
Approximating the Density of Random Differential Equations with Weak Nonlinearities via Perturbation Techniques
Authors: Cortés Juan-Carlos , López-Navarro Elena , Romero José-Vicente , Roselló María-Dolores
Published: January 2021

We combine the stochastic perturbation method with the maximum entropy principle to construct approximations of the first probability density function of the steady-state solution of a class of nonlinear oscillators subject to small perturbations in the nonlinear term and driven by a stochastic excitation. The nonlinearity depends both upon position and velocity, and the excitation is given by a stationary Gaussian stochastic process with certain additional properties. Furthermore, we approximate higher-order moments, the variance, and the correlation functions of the solution. The theoretical findings are illustrated via some numerical experiments that confirm that our approximations are reliable.
New Opportunities for Low Alloy Steels—Master Alloys for Liquid Phase Sintering
Authors: Campos Mónica , Torralba José M. , Oro Raquel de , Bernardo Elena , Galán-Salazar Andrea
Published: January 2021

The Master-alloy (MA) alloy route to promote a liquid phase during sintering has great potential to reduce costs in low alloyed sintered steels, meanwhile enabling the introduction of innovative alloy systems with Cr, Mn and Si. However, in order to successfully modify the performance of steels, multi requirements must be met, including, for example, solubility with the base material, compatibility with the usual sintering atmospheres, homogeneous distribution of the powdered master alloy in the material and the control of secondary porosity. Efforts have been made to properly design the composition of MA, to identify the reducing agents and to understand how they affect the wetting and liquid spreading all over the sintered part. This work reviews these key aspects for the efficient development of steels and explores the possibility to achieve a composition that can act as liquid former or as sinter braze adapting its use to the component requirement.