bluuebunny/crossref_metadata_2025 huggingface dataset:
Arqueología forense en México: del patrimonio a la necesidad de encontrar a los desaparecidos
Authors: Corrales Laura , Beltrán Isabel , Aguayo Sebastián
Published: January 2025

Este artículo es un ejercicio exploratorio sobre el surgimiento del campo laboral de la antropología forense en el marco de la expansión de la violencia del narcotráfico en México. Primero, analizamos la emergencia forense en México y, mediante la comparación entre México y Colombia, observamos el papel de la arqueología en las investigaciones legales. A continuación, a partir de dos carpetas de investigación de rastreo y de una encuesta a diez arqueólogos y arqueólogas, exploramos las circunstancias laborales y los desafíos que enfrentan quienes se desempeñan en el campo forense. Finalmente, planteamos la necesidad de seguir integrando a especialistas en los equipos forenses multidisciplinarios en concordancia con las experiencias de otros países que transitaron por procesos similares.
Ancestros en cuerpos de piedra, sustento del porvenir. Reflexiones en torno a la relación de la comunidad yanakuna con la gente piedra de San Agustín (Huila)
Authors: Salas Rocío , Campo Sayari , Clavijo Jaime Enrique
Published: January 2025

En el macizo colombiano existe una gran cantidad de piedras moldeadas y dispuestas con fines variopintos que la ontología moderna occidental considera como estatuas y objetos arqueológicos sin capacidad de agencia y en disposición única de conservación patrimonial. En contraste, la comunidad indígena yanakuna de San Agustín (Huila) esgrime perspectivas distintas, en las que reconocen simbologías, cuerpo de los ancestros y gente-piedra (rumi runa), fuerzas indispensables para el orden comunitario y la manutención de la vida de todo cuanto habita en el territorio. El artículo desarrolla la perspectiva yanakuna a partir de dos problemas concretos frente al manejo institucional de lo arqueológico. Para ello, analizamos y discutimos desde esa perspectiva indígena las concepciones institucionales y las prácticas en torno a lo patrimonial.
Comunitarismo en torno a la protección integral del patrimonio cultural. explorando metodologías conjuntas en Guanajuato
Authors: Rodríguez Lidia Iris
Published: January 2025

Este artículo presenta un balance del proyecto de divulgación científica realizado con la comunidad de Alfaro en el municipio de León, Guanajuato, México, lacual rodea el sitio arqueológico del Peñón de Alfaro. A través de la vinculación de la arqueología antropológica y la arqueología comunitaria, buscamosidentificar el genius loci del sitio arqueológico, así como el involucramiento de la población con el patrimonio arqueológico a partir de la afectividad.Los resultados permiten identificar una vía de promoción del comunitarismo a partir del disfrute del conocimiento de la historia prehispánica.
Relaciones entre las teorías del Desarrollo, las políticas habitacionales y la planificación urbana. Bogotá, Colombia 1940 – 1990
Authors: Perez Perez Alex Leandro
Published: January 2025

Este artículo recoge las principales reflexiones teóricas sobre las escuelas del desarrollo en el contexto latinoamericano y, a partir de ellas, analiza la evolución de las políticas habitacionales en Colombia y el actuar planificador urbano en Bogotá. En este sentido, se identifican las relaciones entre las políticas socioeconómicas en el contexto nacional y las posturas teóricas sobre el desarrollo; y se resaltan las decisiones gubernamentales y los intereses políticos que han influenciado las decisiones planificadoras de los municipios para la solución del problema habitacional en los sectores de bajos ingresos económicos. Finalmente, se exponen las evidencias de la producción habitacional en Bogotá, influenciada por los paradigmas del desarrollo en la segunda mitad del siglo xx, agrupando por décadas los impactos generados. El propósito de este trabajo es aportar a la discusión sobre la articulación entre la planificación y la calidad del hábitat, y sobre las políticas públicas y sus efectos en el desarrollo urbano de la ciudad.
Historia y valoración patrimonial del Estadio Xalapeño Heriberto Jara Corona
Authors: Castro González Laura
Published: January 2025

A cien años de la construcción del Estadio Xalapeño Heriberto Jara Corona (inaugurado en 1925), este artículo realiza un análisis histórico integral del contexto en el que se gestó y construyó dicho inmueble, junto con una descripción espacial, con el fin de valorarlo como patrimonio cultural, ya que en él se reflejaron los anhelos de modernización posrevolucionarios (en México), la ideas urbanas de la ciudad jardín y la estética de la arquitectura funcionalista. Para lograrlo, en primer lugar se presenta un breve recuento de la relación entre cultura, patrimonio y arquitectura, para luego describir el periodo en que se intervino el lugar conocido como ciénega de Melgarejo, los trabajos previos realizados para construir el Stadium Jalapeño durante el gobierno de Adalberto Tejeda, y los problemas que enfrentó su sucesor, Heriberto Jara, al intervenir, solo tres años después, el mismo espacio urbano. Para ello, se realizó una investigación documental en los archivos históricos del Gobierno del estado de Veracruz y del Ayuntamiento de Xalapa.
In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association
Authors: Perera García Sussy Saymara , Fernández Suárez Kalyanne , Pérez Ortega Eduardo José , Mujica Pérez Yonaisy , Pérez-Pérez Reneé , Rodríguez Yon Yakelin , Haesaert Geert
Published: January 2025

In vitro root cultivation techniques based on modified root systems are often used in studies on Arbuscular Mycorrhizal Fungi (AMF). It is a simplified but powerful tool to investigate AMF root colonization and development of the extraradical mycelium. The aim of this study was to establish and characterize the in vitro culture of a Cuban strain of Rhizophagus irregularis (INCAM 11) by using transformed chicory roots. For that, superficially disinfected propagules of R. irregularis were co-culture with the hairy transformed chicory roots on Modified Strullu and Romand (MSR) medium during five months. Spore germination was observed 3-5 days after surface disinfection. The first contact between AMF hyphae and roots occurred 1 - 3 days after germination and a significant production of extensive extraradical mycelium was observed. New spore formation started within 21 - 25 days. After 5 months, 2000 spores could be observed per plate which were able to germinate, colonize, establish and reproduce again spores when associated to young transformed roots of chicory. The most frequent associated microorganism to the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp.
In vitro propagation of Salvia Pamplonitana: a critically endangered and endemic species of Colombia
Authors: Cancino Escalante Giovanni Orlando , Flórez-Barrera Leidy Johana , Cancino Susan Elsa
Published: January 2025

This study aimed to develop an in vitro regeneration protocol for the propagation and conservation of critically endangered species such as Salvia pamplonitana, an endemic plant of Colombia exclusive to the Pamplona Community. Despite its ornamental potential, S. pamplonitana faces critical endangerment due to habitat destruction caused by human activities such as deforestation, urbanization, and agricultural expansion in densely populated regions. This research addressed the absence of biotechnological studies on S. pamplonitana by developing a methodology to promote in vitro germination and micropropagation, alongside contamination control measures for seed capsules and nodal explants. Successful regeneration of S. pamplonitana was achieved in in vitro from adult plants using nodal segments and seeds collected from a single individual in the University of Pamplona's greenhouse. Surface disinfection treatments for seed capsules demonstrated the significant impact of both NaOCl concentration and exposure duration on seed contamination and germination rates. Furthermore, the evaluation of surface disinfection treatments on nodal segments effectively managed contamination and oxidation levels, highlighting the influence of exposure duration to fungicide, isodine®, and alcohol, with higher concentrations and longer exposure times resulting in increased oxidation and reduced contamination, while emphasizing the widespread use of sodium hypochlorite for surface sterilization. Optimal shoot proliferation was observed with nodal explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l-1 (8.8 µM) 6-Benzylaminopurine and 0.05 mg/l-1 (0.14 µM) gibberellic acid (GA3), while rooting was successful on MS medium supplemented with 3.0 mg/l-1 (14.7 µM) indole butyric acid. The rooted plantlets were acclimatized to ex vitro conditions. Additionally, the study demonstrated enhanced germination through scarification treatment and absence of light, offering potential benefits for ex situ preservation, restoration, and reestablishment of S. pamplonitana populations in their natural habitat.
Cultivo y aplicaciones de Porphyridium cruentum: principales tendencias y proyecciones
Authors: Ramos Frometa Cynthia , Gomez Luna Liliana María
Published: January 2025

Porphyridium cruentum es una microalga que posee múltiples aplicaciones, a partir de la cual se obtienen valiosos bioproductos como la ficoeritrina, y polisacáridos sulfatados. Esta investigación es un estudio exploratorio-descriptivo que realiza una revisión sistemática con herramientas de metanálisis con el objetivo de analizar las tendencias de las investigaciones sobre P. cruentum, su cultivo y principales aplicaciones, con énfasis en el campo magnético (CM). Se conformó una base de datos con 851 artículos entre 1914 - septiembre 2022. En el análisis bibliométrico, se identificaron los principales temas en tendencia y algunos aspectos específicos. Se demostró que existe un marcado interés científico y comercial por P. cruentum, cuyos bioproductos tienen un amplio espectro de aplicaciones, en la industria alimentaria, médico-farmacéutica, energética y cosmética. Los temas relevantes están relacionados con el aumento del rendimiento de bioproductos a gran escala, sin embargo, existe una aplicación limitada del campo electromagnético para dichos fines. Se reporta para la especie que las condiciones de cultivo dependen de los propósitos para los que se utiliza la biomasa. Los cultivos más utilizados son los autotróficos, con iluminaciones de tipo lámparas LED en fotobiorreactores de columnas con burbujeos constantes. Los estudios relacionados con las aplicaciones de campos magnéticos a los cultivos de P. cruentum son limitados, sin embargo, existen reportes de la interferencia positiva de los CM sobre el crecimiento y productividad en algunas especies de microalgas, por lo que se sugiere utilizar el campo magnético estático en la estimulación del crecimiento de P. cruentum.
Capacidad de remoción de nutrientes en aguas residuales por porcinaza por Cyanobacterias Colombianas de alta montaña
Authors: Darwich Cedeño Mohamed , Montenegro Ruiz Luis Carlos , Suarez Pulido Dalia X. , Torres Vargas D. Arley , Gutierrez Jimenez Johan Felipe , Ramirez Tovar Dianne Helain
Published: January 2025

El uso de microalgas se ha planteado como alternativas prometedoras para el tratamiento de aguas residuales a bajos costos, sumado a la producción de oxígeno y reducción de la huella de carbono se pueden obtener productos de valor agregado en el proceso proveniente de las algas. Se pretende evaluar la remoción de nitratos, fosfatos y materia orgánica disuelta en agua residual sintética por primera vez con cianobacterias de alta montaña de Colombia. Se determinó la capacidad de remoción a nivel de laboratorio con aguas residuales sintéticas y a escala reactor con aguas residuales de porcinaza. Las cianobacterias colombianas dulceacuícolas que se utilizaron corresponden al orden de la Synechoccocales y fueron depositadas en la colección del laboratorio de cultivo de algas de la Universidad Nacional de Colombia bajo los códigos LAUN55, LAUN69 y LAUN71. Se comparó la capacidad de biorremediación de las cianobacterias con los tratamientos tradicionales de las microalgas Parachlorelle kessleri y Desmodesmus sp., obteniendo, a nivel laboratorio, rendimientos en remoción de materia orgánica de 85,4% por LAUN71 alcanzando una DBO final de 15,4 mgO2/L-1, mientras que en el tratamiento con Parachlorelle kessleri se alcanzan porcentajes de remoción de 35,2% y una mayor demanda bioquímica de oxígeno (121,1 mgO2/L-1), así mismo reducciones entre 77% y 89% en ion nitrato y reducción entre 86% y 98% en iones fosfato para las cepas de cianobacterias. A escala reactor de 20 L con aguas residuales de porcinaza, Parachlorelle kessleri presentó remoción del 76%, 75%, 75% y 94% para los parámetros de nitratos, fosfatos, DQO y DBO respectivamente, rendimientos significativamente mayores a LAUN71.
Lactic Acid Bacteria as Inhibitory Agents of Escherichia coli ATCC 25922
Authors: Amorocho Cruz Claudia Milena , Ordoñez Lozano Kevy Sandrith
Published: January 2025

In the search to generate new natural alternatives that are less harmful to the body, the use of Lactic Acid Bacteria (LAB) has been chosen due to its ability to grow in different conditions and generate antagonism against other pathogenic microorganisms. Escherichia coli has been the cause of emerging food outbreaks, especially in children and the elderly who have weak immune systems, being a risk to public health. In addition, this pathogen has been demonstrating decreased sensitivity to antibiotics. The present study sought to evaluate the antimicrobial power of 4 LAB strains isolated from food from the Huila-Colombia region against Escherichia coli ATCC 25922 through disc-diffusion and well-diffusion. The strains that inhibited in the presence of cells were L, D (strains isolated from coffee fermentation mass), and 12 (Huila cheese isolate), obtaining inhibition percentages of 37.27±4.15, 32.65±0.97 and 23.47±0.34 for strains L, D and 12, respectively. In the absence of cells, the organic acids of strains L and D obtained from the coffee fermentation process stood out, with inhibition percentages of 27.28±0.27 and 23.63±1.00 respectively. The findings demonstrate the effectiveness of strains native to the region, exalting those obtained from the coffee chain. We performed a multifactorial analysis between LAB and inhibition methods, finding statistically significant differences between methods with p<0.05. In order to know the viability of LAB under in vitro and in vivo conditions, it is important to study other parameters and evaluate the feasibility of using them as a biotherapeutic treatment.
Nueva herramienta biotecnológica para el tratamiento de tumores sólidos cancerosos: transformación genética de bacterias anaerobias
Authors: Martínez Delgado María Alejandra , Bejarano Díaz Stefanny Melissa
Published: January 2025

El cáncer representa una de las principales causas de mortalidad a nivel mundial debido a su comportamiento y a la carga de resistencia a los tratamientos convencionales; esto, ha impulsado a explorar otras alternativas terapéuticas más específicas y con una toxicidad limitada hacia los tejidos circundantes. La terapia contra el cáncer mediada por bacterias se basa en el tropismo inherente de las bacterias por regiones hipóxicas de los tumores sólidos y su capacidad para ser manipuladas genéticamente como un vector para la terapia farmacológica considerándose como un arma potencial contra esta enfermedad, debido a que reducen la tasa de crecimiento de células aberrantes involucrando las respuestas inmunitarias del huésped y las respuestas moleculares celulares. En esta revisión, se presenta esta terapia novedosa haciendo énfasis en los géneros y especies bacterianas más estudiadas y los diversos mecanismos implicados en el direccionamiento y la supresión tumoral.
Patentes en biotecnología a nivel mundial en los últimos cuarenta años
Authors: Restrepo Betancur Luis Fernando
Published: January 2025

El conocimiento del número de patentes publicadas y otorgadas en las diferentes regiones del mundo es de vital interés para los entes de investigación a nivel mundial, ya que permite a los gobiernos establecer estrategias educativas y de inversión, con el objetivo de fomentar el desarrollo biotecnológico y de otras áreas del conocimiento, así como incrementar el número de proyectos, investigaciones e invenciones. En el presente artículo, se tuvo como objetivo efectuar una investigación con base en el número de patentes registradas y otorgadas en las diferentes zonas de clasificación geográfica, con base en la información divulgada por la Organización Mundial de la Propiedad Intelectual (WIPO) referente a aspectos netamente relacionados con el campo de la biotecnología. Se utilizó análisis multivariado de la varianza (MANOVA) con contraste canónico de tipo ortogonal, mediante el modelo lineal general GLM, con transformación de datos basada en la familia BOX-COX; de igual forma se realizó análisis descriptivo exploratorio unidimensional y análisis de correlación por el método de Spearman. Se encontró diferencia altamente significativa entre el continente asiático y las demás zonas, respecto al número promedio de patentes publicadas por año (p<0,0001). África, Latinoamérica y Oceanía ocupan los últimos lugares en el número de patentes publicadas y concedidas. La principal conclusión indica que existe una gran brecha entre Asia, Norte América y Europa respecto a África, Oceanía y Latino América en cuanto al número de invenciones patentadas en el campo de la biotecnología, debido básicamente a factores relacionados con la educación y la economía.
Project Spotlight: National Parks Service building gets world’s 18th LEED gold rating
Authors:   Precast, Prestressed Concrete Institute
Published: January 2025

Surpassing its initial goal of a silver Leadership in Energy and Environmental Design (LEED) rating, the Carl T. Curtis Midwest Regional Headquarters of the National Park Service in Omaha, Neb., became only the 18th building in the world to achieve a gold certification through the United States Green Building Council’s LEED pro- gram and was the first LEED-certified building in Nebraska in July 2004.
Project Spotlight: Precast concrete speed replaces CIP in parking structure
Authors:   Precast/Prestressed Concrete Institute
Published: January 2025

Precast concrete was chosen over cast-in-place for the Tripointe Plaza Garage in Tampa, Fla., in order to successfully complete the garage in a very short time frame while taking into account the likelihood of adverse weather. After precast producer Coreslab Structures (TAMPA) Inc. was given the notice to proceed on the project for the 410-car parking structure on March 3, 2008, it finished drawing, approvals, production, and erection by June 9, 2008.
Výraz vojna v korpusoch slovenského jazyka
Authors: Garabík Radovan , Wachtarczyková Jana
Published: January 2025

V príspevku analyzujeme výskyt lexémy vojna v hlavných slovenských jazykových korpusoch. Zaujíma nás, ako a čím môžu korpusy prispieť k poznaniu jazykovej situácie v konkrétnom lingvokultúrnom priestore, aké informácie prinášajú a ako načrtávajú jazykový obraz sveta. Téma vojny za posledných 30 rokov nevystupovala v slovenskom prostredí do popredia, keďže populácia ju prevažne vnímala ako jav temporálne alebo geograficky vzdialený, teda niečo, čo priamo neovplyvňuje každodenný život.

Zameriame sa na synchrónnu a diachrónnu analýzu v troch hlavných podkorpusoch Slovenského národného korpusu, publicistike, beletrii a odborných textoch, ako aj hovorenom korpuse slovenčiny. Ukazuje sa, že výskyt výrazu vojna a príbuzných lexém (napr. vojak, vojenský, vojnový) je vo webovom korpuse relatívne nízky a predpokladáme, že odráža bežnú jazykovú prax v slovenskom diskurze. Mierne vyšší výskyt je v publicistike a beletrii, pričom v korpuse odborných textov sa tento výraz objavuje ešte častejšie, čo je spôsobené prítomnosťou vedeckých a odborných publikácií z oblasti histórie. Všeobecný hovorený korpus má tiež podobný výskyt ako webový, ale v nahrávkach Ústavu pamäti národa má vojna výrazne vyšší výskyt. Ide o prirodzený odraz orálnej histórie a tzv. malých dejín, ktoré sú písané príbehmi jednotlivcov a pamätníkov historických udalosti (napr. SNP).

Potvrdzuje sa, že dejiny spoločenstiev sú stále písané aj ako dejiny vojen. Pre diachrónnu analýzu sme preskúmali výskyty v niektorých časovo vymedzených korpusoch. V korpuse pokrývajúcom roky 1995 až 1989 je výskyt výrazu vojna výrazne vyšší. V skoršom období rokov 1843 až 1954 je však porovnateľný so súčasným bežným jazykom. Vďakaj anotácii Slovenského národného korpusu sme schopní analyzovať časovú závislosť frekvencie výskytu lexémy v rokoch 1955–2024. Očakávaný predpoklad poklesu týchto výskytov v závislosti od času sa potvrdil len čiastočne, najmä v odborných textoch, v dôsledku nárastu popularity iných vedných odborov. V beletrii a publicistických textoch sa v poslednom desaťročí frekvencia lexémy vojna značne zvýšila. V porovnaní s historickými textami z druhej polovice 19. storočia a prvej polovice 20. storočia sa moderné korpusy ani všeobecný hovorený korpus výrazne nelíšia.

Do analýzy sme zaradili aj vektorové reprezentácie slov a ich vizualizáciu na skúmanie sémantických vzťahov slov. V slovenskom modeli má k výrazu vojna najbližšie spojenie vojnový konflikt a považujeme ho za kontextovú dominantu sémantického poľa pojmu vojna. Reflektujeme aj vektorový prenos z mužského rodu (vojak) do ženského rodu (vojačka). Napriek zjavnej veľmi veľkej rodovej nerovnosti v modernej armáde model nevykazuje rodovo zaujaté výsledky (nižšiu prestíž ženských variantov slov), ako to často vidíme v anglickojazyčných modeloch (v slovenských tento jav nebol potvrdený). Na záver ukazujeme možnosť použitia veľkého jazykového modelu na generovanie lexikografických definícií ako príklad možného budúceho smerovania lexikografie.
Od Čechů k Cikánům a zpátky. Antonín Jaroslav Puchmajer, jeho Románi Čib a program národního obrození
Authors: Šefčík Ondřej
Published: January 2025

České národní obrození je ve svých prvních dvou generacích (jak je klasifikoval Miroslav Hroch) primárně orientováno filologicky: obě generace se filologii věnovaly programově a důsledně, věřily totiž, že právě gramatika, lexikon a literatura aktivně psaná v národním jazyce jsou samy o sobě základním a nejdůležitějším vnějším znakem samotné existence národa (tomuto zaměření můžeme říkat filologický obrat). Klíčový vliv na dobovou českou gramatologii pak měla zásadní a inovátorská česká gramatika Josefa Dobrovského, která byla často metodologicky i formálně napodobována dalšími obrozenci stejné i mladší generace. Jedním z nejpozoruhodnějších děl tohoto typu je gramatika cikánského jazyka Románi Čib, kterou německy (a částečně česky a ovšem také romsky) napsal kněz, básník, národní obrozenec Antonín Jaroslav Puchmajer). Posmrtně vydaná Románi Čib je vůbec pozoruhodným jazykovědným dílem, které se vymyká obvyklému poli zájmu českého obrozeneckého hnutí, neboť je zaměřeno na neslovanský jazyk, navíc marginalizované národnosti. Jde o gramatiku v dobovém kontextu velmi moderní, přiznaně vzniklou pod metodologickým vlivem gramatiky Josefa Dobrovského, doplněnou dále romským slovníčkem a čítankou, navíc pak slovníčkem zlodějské jazyka hantýrky, kterou Puchmajer důsledně odlišuje od vlastní romštiny. Rozsah svazku je přitom relativně skromný (pouhých 88 stran!). Kromě toho je Románi Čib ale také (patrně podvědomě) miniaturou filologického programu národního obrození 19. století, jak jej právě vypracovali (a úspěšně využívali) čeští národní obrozenci první a druhé generace, má totiž všechny vlastnosti, které byly v dobovém kontextu považovány za zásadní pro každý národní program. Puchmajerova Románi Čib obsahuje všechny tři části programu: vyjma gramatiky a slovníčku pak čítanka obsahuje nejen vůbec první publikovanou ukázku původních romských veršů, ale také ukázku překladu Nového zákona a osmnáct bajek v romštině, které napsal sám Puchmajer. Důležité je i „etnogenetické“ pojetí předmluvy: Puchmajer v ní ostře vyděluje Romy (které chápe jako česko-moravsko-uherskou zvláštnost) od německých Sintů, a tím také vlastně inkorporuje Romy do širšího těla vlastního národa. Vzhledem k tomu, že jím popsaný český dialekt už neexistuje (zanikl během druhé světové války, moderní čeští Romové přišli ze Slovenska po válce), je Románi Čib také specifickým etnografickým dokladem sui generis. Pozoruhodný je i obecně humanistický (byť kritický) pohled na Romy, ve kterém autor zdůrazňuje i mravní převahu „čistokrevných“ Romů (kterým říká Kálo) nad „míšenci“ (Párno). Puchmajerovo Románi Čib zůstává pomníkem nejen dobové gramatologie, ale i pomníkem ztracených českých Romů.
Business Penetration in the Development of e-Government Concepts: Challenges of Managing Cloud-Based Website Computing Systems
Authors: Dewi Dian Suluh Kusuma , Harsono Jusuf , Desriyanti Desriyanti , Yulianti Dwiana Binti , Zainuddin Arif
Published: January 2025

In the digital era, e-government initiatives have revolutionised how governments interact with businesses and citizens. This study aims to explore the integration of business processes into e-government models through the implementation of cloud computing-based systems, with a specific focus on the tourism sector in Ponorogo Regency. Using a qualitative methodology that includes documentation review, in-depth interviews, and Focus Group Discussions (FGD), the study identifies key challenges in managing the dolanngebel.com website. The findings highlight significant issues in transparency, participation, and collaboration among government entities, business stakeholders, and the public. These challenges are attributed to organisational inefficiencies, limited human resources, financial constraints, and regulatory hurdles. The study concludes that addressing these issues could improve administrative efficiency, stakeholder engagement, and public trust in e-government services. This research offers original insights into the practical challenges and opportunities of integrating e-government and business models, particularly in the tourism industry, and provides actionable recommendations for policymakers and practitioners.
Field Epidemiology Training Program (FETP) — Frontline 3.0: Using a One Health Approach
Authors: Gregory Elizabeth
Published: January 2025

"From the letter by Dr. Benjamin Park: “On behalf of the U.S. Centers for Disease Control and Prevention (CDC), it is our privilege to present the third version of the Field Epidemiology Training Program (FETP)—Frontline 3.0. This document reflects our shared commitment to strengthening public health systems worldwide by building the capacity of frontline health workers to rapidly detect, respond to, and contain health threats, now with an increased emphasis on a One Health approach. One Health is a collaborative, multisectoral, and transdisciplinary approach—working at the local, regional, national, and global levels—with the goal of achieving optimal health outcomes recognizing the interconnection between people, animals, plants, and their shared environment….
“FETP–Frontline 3.0 emphasizes real-world, hands-on training, equipping participants with practical skills to conduct surveillance, analyze data, and implement timely interventions. We recognize that each country brings unique strengths and faces distinct challenges. The guidelines provided here aim to support the seamless integration of FETP–Frontline 3.0 into your existing public health frameworks, tailoring it to your specific needs and priorities while fostering cross-sectoral collaboration inherent to the One Health approach. We encourage you to use this resource as a roadmap, adapting it to optimize outcomes for building safer communities….”
All of the FETP 3.0 materials are contained in a single Zip folder named “FETP_Frontline_3_One_Health_Approach_20250123.zip”, found under the “Supporting Files” header, near the bottom of this webpage. The zip folder contains the following folders and materials: Welcome to FETP 3.0 Letter from Dr. Benjamin Park; FETP-Frontline 3.0 Implementation and Curriculum Guide; Field Intervals Guide for Mentors; folder a. Assessments; folder b. Workshop 1; folder c. Field Interval 1; folder d. Workshop 2; folder e. Field Interval 2; and folder f. Workshop 3. These folders contain various curriculum slide decks and documents."
Skuteczność systemu ochrony zabytków sakralnych w XIX i XX w. na przykładzie losów kościoła pod wezwaniem św. Jana Chrzciciela w Komorowicach
Authors: Skrzypek Miłosz
Published: January 2025

The construction and maintenance of churches have always been an important aspect of parish life, both in the past and in the present. This process, while reflecting the development of the local community and vibrant religious life, presents a number of challenges, particularly of a financial and organizational nature. Additional difficulties arise when it is necessary to find a new use for an existing church, especially if it is a listed monument.The Parish of St. John the Baptist in Komorowice faced similar issues for nearly a century. The fate of its historic church, described in the article, serves as an example of the complexity and challenges associated with the emerging monument protection system in the 19th century. A key barrier was financial constraints, and the lack of effective legal tools to enforce conservation duties further complicated the situation.Despite these difficulties, awareness of the need to protect monuments was already strong in the 19th century, even in rural areas. The residents of Komorowice were eager to contribute financially to the upkeep of the historic church, provided that the requirements were adapted to their capabilities and bureaucratic obstacles did not hinder efforts. The article encourages reflection on whether the approach to monument protection has improved in later years.
Obrazowanie postaci św. Jana z Kęt w łacińskim i polskim kaznodziejstwie doby baroku
Authors: Rowińska-Szczepaniak Maria
Published: January 2025

St. John Cantius – a student and professor of the Krakow Academy – holds a significant place in Old Polish writings. Literary forms preserving the cult of Master John included Latin and Polish sermons, which were initially written to celebrate the anniversaries of the saint’s death, and then his liturgical memorial day. The method of the representation of St. John Cantius in selected Baroque sermons is the subject of the analysis carried out in this article. The use of light-related metaphors by their authors, for instance, by referring to John Cantius as a sun, star, lamp, torch, or fire, is the common element of the analyzed texts. Undoubtedly, the application of such metaphors has its source in the Christian philosophical and theological thought. Moreover, it is a manifestation of paravisual tendencies and fascination with the sun, typical of the literature of the Baroque period.
Hausdorff Objects
Authors: Baran Mehmet
Published: January 2025

In previous papers, several extensions of the T2 separation property in topology to a
topological category were compared. The aim of this paper is to develop further results
relating to these extensions as well as to solve several unsolved problems. Moreover, we
show one of these T2, namely KT2 limit spaces and reciprocal limit spaces are equivalent
and every KT2 limit space induces the associated complete uniform limit space. Finally,
we compare our results and give some applications.
Pseudo-differential operators associated with Gyrator transform
Authors: Mahato Kanailal , Arya Shubhanshu , Prasad Akhilesh
Published: January 2025

In this present work, a brief introduction to the Gyrator transform and its fundamental properties are given. Gyrator transform of tempered distribution is studied. This article made further discussion on the boundedness properties of pseudo-differential operators associated with Gyrator transform on Schwartz space as well as on Sobolev space.
Another perspective on Kannan contraction
Authors: Cvetković Marija
Published: January 2025

Inspired by the well-known result stating that if any iterate of a mapping is a Banach contraction on a complete metric space, then the mapping itself possesses a unique fixed point, we investigate that claim for Kannan contraction but by retaining the left-hand side of the inequality as per the mapping itself. With an additional assumption of k -continuity, the existence and uniqueness of a fixed point is obtained for a new class of contractions, m-Kannan contraction, on a complete metric space. Several examples are given in order to substantiate many theoretical claims such as discontinuity at the unique limit point of the iterative sequence or the ones testifying that this class is wider than the class of Kannan mappings.
SYSTEM ANALYSIS AND DESIGN OF THE GAME «SHOOTING SIMULATION IN A SHOOTING RANGE»
Authors: Yanchev D. , Sakaliuk O. , Popkov D.
Published: January 2025

The article examines the system analysis and design of the game «Shooting Simulation in a Shooting Range», which is a relevant area in the context of the modern gaming industry’s development. The research emphasizes the importance of using information technologies to create interactive products that combine high levels of graphic detail, realistic physical models, and user-friendly interfaces. The main purpose of the study is to develop software for a shooting simulation using modern tools and methods. The research identifies the key system requirements, describes the stages of project implementation, and proposes approaches to solving the main technical challenges.
The paper provides a detailed explanation of the choice of development tools, specifically the use of the Unreal Engine 5 game engine and the Blender 3D editor. Unreal Engine 5 ensures high-quality graphics, efficient visual programming tools through the Blueprint system, and comprehensive developer support. Blender, as a powerful tool for creating three-dimensional models, was used to design objects such as targets and weapons. The choice of these tools is justified by their accessibility, functionality, and active community support.
The game development methodology includes several stages: requirements analysis, architecture and interface design, code development, testing, and debugging. During development, modern approaches to modeling physical properties were applied, ensuring realistic simulation of shooting, including aspects such as bullet trajectory, object interactions, and acoustic effects. Realistic sound was achieved through the integration of high-quality audio files, enhancing the overall gaming experience.
Special attention was given to the practical implementation of the game’s core features, including weapon selection, target customization, the use of optical sights, and the implementation of bonus shots under certain conditions. An important component is the interactive settings menu, allowing players to individually adjust game parameters. The game was tested on various hardware platforms, confirming its compatibility with a wide range of devices.
The results of the study demonstrate the potential use of this product for both entertainment and training purposes. The shooting simulation can be beneficial for training in shooting disciplines and engaging users with top-tier gaming mechanics. The work underscores the importance of integrating innovative technologies into the process of creating gaming products that meet modern standards of quality and convenience. Thus, the presented development serves as an example of the successful application of modern technologies in the field of interactive entertainment and demonstrates the prospects for developing such projects in the context of the growing popularity of virtual games and simulators.
NON-TOXIGENIC VIBRIO CHOLERAE O1 STRAINS CAUSED GASTROENTEROCOLITIS IN THE ZAPORIZHIA AND KHERSON REGIONS: CASE REPORTS AND PATHOGEN MOLECULAR GENETIC CHARACTERISTICS
Authors: Monakhova Elena V. , Kruglikov Vladimir D. , Vodop'yanov Aleksey Vodop'yanov S. , Gaevskaya Natalia E.
Published: January 2025

In 2023-2024, two cases of gastroenterocolitis caused by non-toxigenic Vibrio cholerae O1 were documented in Zaporizhzhya and Kherson regions. Previously, diseases of this etiology had not been detected in Russia over the last 18 years, although CTX- strains were isolated from water bodies. Aim of the study: molecular genetic characterization of clinical V.cholerae O1 strains isolated in Russia and comparative bioinformatics analysis of their whole genome sequences (WGSs). Materials and methods. The strains were identified in accordance with MUK 4.2.3745-22, sequencing was performed on the MiSeq Illumina platform, bioinformatics analysis – using BioEdit, Vector NTI, CARD and author’s software. Results. A patient from Zaporizhzhya region fell ill after consumption of market foods, and from the Kherson region – after bathing in the sea. Both were diagnosed with acute gastroenterocolitis, in the second case rotavirus was detected along with vibrio. The isolated cultures with identical PCR genotypes were identified as non-toxigenic V. cholerae O1. The first pathogen belonged to Ogawa serotype, the second to Inaba. The WGSs of both strains lacked prophages CTX, preCTX, islands VPI and VSP-II, genes of heat-stable and cholix toxins, proteins OmpT and OmpU, but shared RTX and msh clusters, genes of hemolysin, metallo- and serine proteases; cytotonic factor Cef, represented in two pathogens by different alleles. Significant differences included the presence in the 2024 pathogen of VSP-I island, T3SS cluster integrated into VPI-3, ompW and β-lactamase varG genes which were absent in the 2023 one. The latter contained an incomplete VPI-2 and significantly altered genes rtxA, as well as genes encoding proteases VesC and VchC . On the dendrogram, the isolates formed 2 distant clusters, which also included environmental waterborne strains isolated in other Russian territories in different years; in each cluster all genes had the same repertoires of genetic determinants and gene alleles. These data evidence an opportunity for their long-term preservation in water reservoirs and the absence of importations from abroad. Conclusion. Two new clonal complexes of CTX- V. cholerae O1 have been identified in the territory of Russian Federation. Non-toxigenic strains usually do not cause large-scale epidemiological complications, and the diseases caused by representatives of each complex could be associated with compromised immunity of patients.
REGULATION OF IMMUNE RESPONSE BY PERMAFROST MICROORGANISM METABOLITES VIA EFFECTOR T-LYMPHOCYTES
Authors: Petrov Sergei Anatolievich , Sukhovey Yuri Gennadievich , Kalyonova Lyudmila Fedorovna , Kostolomova Elena Gennadievna , Kastornov Alexander Alexandrovich
Published: January 2025

Infectious agents have closely interacted with the human immune system, acquiring a set of highly sophisticated mechanisms for modulating immunity. One of the survival strategies for viruses, bacteria, protozoa, helminths and fungi is to target the regulatory T cell network (Treg: CD4+CD25hiCD127-) that controls immunopathogenic responses in many infections. Not only pathogens but also commensals are able to directly induce the conversion of naive T cells into suppressive Foxp3-expressing Tregs, while others activate pre-existing natural Tregs, in both cases suppressing pathogen-specific effector responses. However, Tregs can also contribute to immunity under certain conditions, such as at the initial stages of infection when effector cells must gain access to the site of infection, and subsequently in ensuring the generation of effector memory cells. It is noteworthy that currently little information on whether infections selectively drive pathogen-specific Tregs, and if so, whether such cells are also reactive to autoantigens are available. Further analysis of Treg subset specificity, along with a clearer picture of relative dynamics during the disease, should lead to rational strategies of immune intervention to optimize immunity and eliminate the infectious process. Thus, restoration of Treg function is important in the treatment of infectious, autoimmune and other diseases and can serve as a marker of their successful treatment. The article assesses the effect of exometabolites of derived from permafrost Bacillus bacteria obtained at different temperature conditions of their cultivation on the activity of Treg and effector T lymphocyte differentiation. Significant differences were established: secondary microbial exometabolites affect Treg (CD4+CD25hiCD127-) differentiation and expression of activation markers (CD69, CD25, HLA-DR) on CD4+ and CD8+ T lymphocytes. This effect is regulated by the type of metabolites obtained at different temperatures - "cold" (obtained at 5°C of bacterial incubation), "medium-temperature" (at 22°C) and "heat" (at 37°C) metabolites. In this case, an increase in the Treg level is associated with lower differentiation activity of CD4+ T-lymphocytes exposed to “cold” secondary exometabolites, a decrease in the differentiation activity of CD8+ T-lymphocytes treated with “warm” secondary exometabolites, and a roughly equivalent effect on the differentiation activity of CD4+ and CD8+ T-lymphocytes acted upon by “medium-temperature” secondary exometabolites.
Hydrometeorological conditions of Avacha Bay during the period of monitoring studies in 2013–2022
Authors: Tepnin O. B.
Published: January 2025

Interannual variability of hydrometeorological conditions in Avacha Bay was analyzed on the basis of data obtained in the course of complex standard surveys in the period from 2013 to 2022. Steady excess of climatic norm for air temperature and precipitation is shown in the area mentioned throughout the entire period of the hydrological studies. The air temperature, however, experienced significant interannual fluctuations in the considered range of years, while precipitation levels increased noticeably only in the period from 2013 to 2015, and then there was a tendency to a slow decline with a minimum in 2022. The surface atmospheric pressure exceeded the multiyear average in summer and autumn, but was below it in winter. Ten-year averages of temperature, salinity, chlorophyll-α concentration, and oxygen saturation for April through October at 10 stations on standard horizons were calculated. Analysis of interannual variability of the temperature and salinity integral indices for the entire studied water column of Avacha Bay has demonstrated a gradual increase of heat content and a slight decrease of salinity.
Desempenho do “a body shape index” como discriminador de obesidade e obesidade sarcopênica - ELSA-Brasil
Authors: Silva Jane Kellen , Aguiar Odaleia Barbosa de , Diniz Maria de Fátima Haueisen Sander , Braga José Ueleres , Griep Rosane Harter , Fonseca Maria de Jesus Mendes da
Published: January 2025

Resumo Objetivou-se determinar o desempenho diagnóstico do ABSI para obesidade e obesidade sarcopênica, comparados aos resultados da bioimpedância (BIA) e do IMC, por sexo e faixa etária. Trata-se de um estudo seccional com 12.793 participantes da segunda onda do ELSA-Brasil, que obteve medidas de percentual de gordura corporal utilizando BIA e antropometria, verificando-se a performance dos testes diagnósticos para comparar os índices. Os resultados mostraram que, para obesidade, em homens nas três faixas etárias, a sensibilidade foi abaixo de 49%. A especificidade variou de 73% a 93%, sendo maior na faixa etária de 65 a 79 anos. Esse padrão foi encontrado para as mulheres, sendo a sensibilidade inferior a 42%; a especificidade variou entre 85% e 92%, sendo maior nas idosas. Quanto à obesidade sarcopênica, a sensibilidade e a especificidade entre os homens nas três faixas etárias foram de 72% e 99%, respectivamente. Entre as mulheres, a sensibilidade foi de aproximadamente 95%, e a especificidade em torno de 70% em todas as faixas etárias. O ABSI, comparado à BIA, mostrou reduzida capacidade de discriminar obesidade, mas mostrou-se bom marcador de obesidade sarcopênica.
Construction and validation of an instruction manual of nutritional care for severely obese people in the Unified Health System
Authors: Dias Mariana de Moura e , Coelho Olívia Gonçalves Leão , Cândido Flávia Galvão , Hermsdorff Helen Hermana Miranda
Published: January 2025

Abstract This article describes the construction and validation of an instruction manual geared toward nutritional care (NC) for people with severe obesity in the Brazilian Unified Health System (SUS). In the production of this instruction manual, a broad literature review was conducted for the identification and discussion of topics to be treated. The content and appearance validity were conducted according to the Delphi technique and to focus groups, respectively, with evaluators who were nutritionists and practitioners, from different regions of Brazil. According to the Delphi technique, the instruction attributes and their content as a whole were properly and sufficiently evaluated according to values obtained for the Content Validity Index (CVI > 0.8). In the focus groups, the saturation of content was reached. Furthermore, discussions considering the reality of SUS were conducted and subsequently incorporated into the instructions in order to adequate the final version of the instructions to SUS professionals’ needs. In conclusion, the instruction manual produced to fill the gap regarding nutritional care for people with severe obesity presents an adequate content in terms of both quality and topics of interest (content validation), and is applicable within the reality of SUS nutritionists (appearance validity).
Between the streets and the RAPS: Integrative review on the access of the homeless population to Mental Health Services
Authors: Fraga Priscilla Victória Rodrigues , Araújo Maria Cecília Assis de , Souza Anelise Andrade de , Martins Ana Luísa Jorge , Dantas Ana Carolina de Moraes Teixeira Vilela , Marinho Rafaela Alves , Rodrigues Diadorim Maria , Paes-Sousa Rômulo
Published: January 2025

Abstract This integrative review systematized the factors that influence access to mental health services for the Homeless Population (HP) in harmful use of alcohol and other drugs in the Psychosocial Care Network (RAPS) in Brazil by categorizing the factors into access “barriers” and “facilitators”. We selected 13 corresponding articles and subsequently assessed their methodological quality. We identified 19 access barriers and 22 access facilitators, observing a convergence and complementarity of the factors identified, with no disagreements between authors. Although there are specific barriers and facilitators related to substance use, most are connected to HP’s conditions and lifestyles or how services address the HP. Discrimination, bureaucratic rigidity of services, and a lack of intra- and intersectoral integration were identified as significant barriers. On the other hand, Street Outreach Clinics and practices such as harm reduction and matrix-based support emerged as notable facilitators, adapting to patients’ needs. Future studies should explore specific factors and the relationships between these factors.