bluuebunny/crossref_metadata_2025 huggingface dataset:
The Synergistic Effect of Pore Architect and Reducibility in Ceria-Promoted Ni Molecular Sieve for Methane Dry Reforming
Authors: Alwadai Norah , Abahussain Abdulaziz A. M. , Shrivastava Vijay Kumar , Al-Zahrani Salma A. , Fakeeha Anis H. , Alarifi Naif , Bayazed Mohammed O. , Banabdwin Khaled M. , Kumar Rawesh , Al-Fatesh Ahmed
Published: November 2024

Methane and carbon dioxide, the primary contributors to global warming, are now at critical levels, threatening the extinction of numerous organisms on our planet. In this regard, dry reforming of methane reactions have gained considerable attention because of the conversion capacity of CH4 and CO2 into synthetic/energy-important syngas (H2 and CO). Herein, a molecular sieve (CBV3024E; SiO2/Al2O3 = 30) with ZSM-8-type pore architect, is utilized as the support for the active site of Ni and Ce promoters. Catalysts are characterized by surface area and porosity, X-ray diffraction study, Raman and infrared spectroscopy, thermogravimetry analysis, and temperature-programmed reduction/desorption techniques. A total of 2 wt.% ceria is added over 5Ni/CBV3024E to induce the optimum connectivity of aluminum in the silicate framework. NiO residing in these porous cages are mostly under “prominent interaction with support” which is reduced easily into metallic Ni as the active sites for DRM reactions. The active sites over 5Ni2Ce/CBV3024E remain stable during the DRM reaction and achieve ~58% H2 yield after 300 min TOS at 42,000 mL/(gcat.h) GHSV and ~70% H2 yield after 20 h at 26,000 mL/(gcat.h) GHSV. The high activity after a longer time stream justifies using CBV3024E molecular sieves as the support and ceria as the promoter for Ni-based catalyst towards the DRM reaction.
Analysis of Reaction Conditions in Palmitic Acid Deoxygenation for Fuel Production
Authors: Ferreira Karoline K. , Ribeiro Lucília S. , Pereira Manuel Fernando R.
Published: November 2024

The development of effective catalytic systems for deoxygenation reactions is critical to the conversion of renewable feedstocks into sustainable fuels. In this work, the influence of various reaction parameters on the conversion of palmitic acid into alkanes, such as temperature, stirring rate, reaction time, H2 pressure, amount of catalyst and substrate concentration was evaluated using the commercial Co-Mo/Al2O3 catalyst. In parallel, bimetallic Co-Mo catalysts supported on carbon nanotubes (CNTs) were prepared and characterized using various techniques, and their catalytic performance was assessed under the optimized conditions. The results showed that palmitic acid can be efficiently converted at 350 °C for 6 h at 30 bar H2 pressure, stirring at 150 rpm and using 0.25 g of catalyst and 0.50 g of palmitic acid in 50 mL of n-decane. Under these conditions, a complete substrate conversion and yields of 89.4 and 4.8% of C16 and C15 were achieved. In addition, Co-Mo/CNTox presented a similar catalytic performance as the commercial one, with a final result of 90.9% yield in C16. These findings point out the potential of using Co-Mo/CNTox as a competitive alternative to liquid fuel production.
Progress in the Synthesis and Applications of C3N5-Based Catalysts in the Piezoelectric Catalytic Degradation of Organics
Authors: Yin Shupeng , Yu Huiguo , Fu Haifeng , Wang Yinglong , Meng Fanqing
Published: November 2024

Piezoelectric catalysis has shown great potential for application in green chemistry due to its “clean” properties. By applying external mechanical force, this method can induce rapid charge transfer, providing an important reaction pathway for carbon neutrality and carbon peaking. Carbon nitride (C3N5)-based catalysts, as a novel material, have received widespread attention for their synthesis and application in the piezoelectric catalytic degradation of organic compounds. This review summarizes the latest research progress of C3N5-based catalysts, covering their applications in environmental governance and resource utilization, including the removal of organic pollutants in water. We focused on the synthesis strategy, characterization methods, and application progress of C3N5-based catalysts in the degradation of organic pollutants. The quantitative results show that some C3N5-based catalysts had removal efficiencies of over 85% in the treatment of specific pollutants. In addition, this article also discusses the piezoelectric effect and its degradation mechanism, providing direction for future research. Finally, the application prospects and potential development directions of C3N5-based catalysts in environmental governance are discussed.
Visible-Light-Activated TiO2-Based Photocatalysts for the Inactivation of Pathogenic Bacteria
Authors: Haque Farhana , Blanchard Allison , Laipply Baileigh , Dong Xiuli
Published: November 2024

Pathogenic bacteria in the environment pose a significant threat to public health. Titanium dioxide (TiO2)-based photocatalysts have emerged as a promising solution due to their potent antimicrobial effects under visible light and their generally eco-friendly properties. This review focuses on the antibacterial properties of visible-light-activated, TiO2-based photocatalysts against pathogenic bacteria and explores the factors influencing their efficacy. Various TiO2 modification strategies are discussed, including doping with non-metals, creating structure defects, combining narrow-banded semiconductors, etc., to extend the light absorption spectrum from the UV to the visible light region. The factors affecting bacterial inactivation, and the underlying mechanisms are elucidated. Although certain modified TiO2 nanoparticles (NPs) show antibacterial activities in the dark, they exhibit much higher antibacterial efficacies under visible light, especially with higher light intensity. Doping TiO2 with elements such as N, S, Ce, Bi, etc., or introducing surface defects in TiO2 NPs without doping, can effectively inactivate various pathogenic bacteria, including multidrug-resistant bacteria, under visible light. These surface modifications are advantageous in their simplicity and cost-effectiveness in synthesis. Additionally, TiO2 can be coupled with narrow-banded semiconductors, resulting in narrower band gaps and enhanced photocatalytic efficiency and antibacterial activities under visible light. This information aids in understanding the current technologies for developing visible-light-driven, TiO2-based photocatalysts and their application in inactivating pathogenic bacteria in the environment.
CO Oxidation over Cu/Ce Binary Oxide Prepared via the Solvothermal Method: Effects of Cerium Precursors on Properties and Catalytic Behavior
Authors: Jin Wen , Liu Yanmin , Xue Hongyan , Yu Jun , Mao Dongsen
Published: November 2024

Cu/Ce binary oxides were prepared via the one-pot solvothermal method, and the effects of different cerium precursors (cerium nitrate and cerium ammonium nitrate) on the catalytic activity and resistance to water vapor or CO2 of the prepared samples for low-temperature CO oxidation reaction were investigated. The physicochemical characteristics of the catalysts were characterized via thermal analyses (TG-DSC), X-ray diffraction (XRD), Raman spectroscopy, N2 adsorption/desorption, inductively coupled plasma-atomic emission spectrometry (ICP-AES), X-ray photoelectron spectroscopy (XPS), in situ diffuse reflectance infrared Fourier transform spectroscopy (in situ DRIFTs), and temperature-programmed reduction with H2 (H2-TPR). The results indicated that the CuO/CeO2 catalyst (CC-N) prepared with cerium nitrate showed higher activity for low-temperature CO oxidation, which can be ascribed to its larger specific surface area and pore volume, higher amounts of highly dispersed CuO species with strong interaction with CeO2, Cu+ species, and more active surface oxygen species, compared with the counterpart prepared with cerium ammonium nitrate (CC-NH). Furthermore, the CC-N catalyst also exhibited better resistance to CO2 poisoning than CC-NH.
High Mobility Group Box 1 (HMGB1): Molecular Signaling and Potential Therapeutic Strategies
Authors: Datta Sayantap , Rahman Mohammad Atiqur , Koka Saisudha , Boini Krishna M.
Published: November 2024

High Mobility Group Box 1 (HMGB1) is a highly conserved non-histone chromatin-associated protein across species, primarily recognized for its regulatory impact on vital cellular processes, like autophagy, cell survival, and apoptosis. HMGB1 exhibits dual functionality based on its localization: both as a non-histone protein in the nucleus and as an inducer of inflammatory cytokines upon extracellular release. Pathophysiological insights reveal that HMGB1 plays a significant role in the onset and progression of a vast array of diseases, viz., atherosclerosis, kidney damage, cancer, and neurodegeneration. However, a clear mechanistic understanding of HMGB1 release, translocation, and associated signaling cascades in mediating such physiological dysfunctions remains obscure. This review presents a detailed outline of HMGB1 structure–function relationship and its regulatory role in disease onset and progression from a signaling perspective. This review also presents an insight into the status of HMGB1 druggability, potential limitations in understanding HMGB1 pathophysiology, and future perspective of studies that can be undertaken to address the existing scientific gap. Based on existing paradigm of various studies, HMGB1 is a critical regulator of inflammatory cascades and drives the onset and progression of a broad spectrum of dysfunctions. Studies focusing on HMGB1 druggability have enabled the development of biologics with potential clinical benefits. However, deeper understanding of post-translational modifications, redox states, translocation mechanisms, and mitochondrial interactions can potentially enable the development of better courses of therapy against HMGB1-mediated physiological dysfunctions.
Mucosal Inflammatory Memory in Chronic Rhinosinusitis
Authors: Koo Min-Seok , Moon Sungmin , Rha Min-Seok
Published: November 2024

Recent advancements in medical management, endoscopic sinus surgery, and biologics have significantly improved outcomes for patients with chronic rhinosinusitis (CRS). However, long-term recurrence is frequently observed following endoscopic sinus surgery, with symptoms worsening after biologics are discontinued. Consequently, refractory or recurrent CRS remains a significant challenge, causing a substantial healthcare burden. In this review, we provide current insights into mucosal inflammatory memory, a potential mechanism leading to CRS recurrence. Given that both immune and non-immune cells in the sinonasal mucosa play critical roles in the pathophysiology of CRS, a deeper understanding of the mechanisms underlying mucosal inflammatory memory in various cellular components of sinonasal tissue could aid in the management of refractory CRS. We describe and discuss the latest knowledge regarding the novel concept of inflammatory memory, including both adaptive immune memory and trained immunity. Additionally, we summarize the pathogenic memory features of the sinonasal mucosa cellular components in the context of CRS.
The Molecular Mechanism of Farnesoid X Receptor Alleviating Glucose Intolerance in Turbot (Scophthalmus maximus)
Authors: Qin Gaochan , Pan Mingzhu , Huang Dong , Li Xinxin , Liu Yue , Yu Xiaojun , Mai Kangsen , Zhang Wenbing
Published: November 2024

To explore the molecular targets for regulating glucose metabolism in carnivorous fish, the turbot (Scophthalmus maximus) was selected as the research object to study. Farnesoid X receptor (FXR; NR1H4), as a ligand-activated transcription factor, plays an important role in glucose metabolism in mammals. However, the mechanisms controlling glucose metabolism mediated by FXR in fish are not understood. It was first found that the protein levels of FXR and its target gene, small heterodimer partner (SHP), were significantly decreased in the high-glucose group (50 mM, HG) compared with those in the normal glucose group (15 mM, CON) in primary hepatocytes of turbot. By further exploring the function of FXR in turbot, the full length of FXR in turbot was cloned, and its nuclear localization function was characterized by subcellular localization. The results revealed that the FXR had the highest expression in the liver, and its capability to activate SHP expression through heterodimer formation with retinoid X receptor (RXR) was proved, which proved RXR could bind to 15 binding sites of FXR by forming hydrogen bonds. Activation of FXR in both the CON and HG groups significantly increased the expression of glucokinase (gk) and pyruvate kinase (pk), while it decreased the expression of cytosolic phosphoenolpyruvate carboxykinase (cpepck), mitochondrial phosphoenolpyruvate carboxykinase (mpepck), glucose-6-phosphatase 1 (g6pase1) and glucose-6-phosphatase 2 (g6pase2), and caused no significant different in glycogen synthetase (gs). ELISA experiments further demonstrated that under the condition of high glucose with activated FXR, it could significantly decrease the activity of PEPCK and G6PASE in hepatocytes. In a dual-luciferase reporter assay, the FXR could significantly inhibit the activity of G6PASE2 and cPEPCK promoters by binding to the binding site ‘ATGACCT’. Knockdown of SHP after activation of FXR reduced the inhibitory effect on gluconeogenesis. In summary, FXR can bind to the mpepck and g6pase2 promoters to inhibit their expression, thereby directly inhibiting the gluconeogenesis pathway. FXR can also indirectly inhibit the gluconeogenesis pathway by activating shp. These findings suggest the possibility of FXR as a molecular target to regulate glucose homeostasis in turbot.
Cocaine-Induced DNA-Dependent Protein Kinase Relieves RNAP II Pausing by Promoting TRIM28 Phosphorylation and RNAP II Hyperphosphorylation to Enhance HIV Transcription
Authors: Sharma Adhikarimayum Lakhikumar , Tyagi Priya , Khumallambam Meenata , Tyagi Mudit
Published: November 2024

Drug abuse continues to pose a significant challenge in HIV control efforts. In our investigation, we discovered that cocaine not only upregulates the expression of the DNA-dependent protein kinase (DNA-PK) but also augments DNA-PK activation by enhancing its phosphorylation at S2056. Moreover, DNA-PK phosphorylation triggers the higher localization of the DNA-PK into the nucleus. The finding that cocaine increases the nuclear localization of the DNA-PK provides further support to our observation of enhanced DNA-PK recruitment at the HIV long terminal repeat (LTR) following cocaine exposure. By activating and facilitating the nuclear localization of the DNA-PK, cocaine effectively orchestrates multiple stages of HIV transcription, thereby promoting HIV replication. Additionally, our study demonstrates that the cocaine-induced DNA-PK promotes the hyper-phosphorylation of the RNA polymerase II (RNAP II) carboxyl-terminal domain (CTD) at Ser5 and Ser2 sites, enhancing both the initiation and elongation phases, respectively, of HIV transcription. The cocaine-mediated enhancement of transcriptional initiation is supported by its activation of cyclin-dependent kinase 7 (CDK7). Additionally, the induction of transcriptional elongation is marked by higher LTR recruitment and the increased phosphorylation of CDK9, which indicates the stimulation of positive transcriptional elongation factor b (P-TEFb). We demonstrate for the first time that cocaine, through DNA-PK activation, promotes the specific phosphorylation of TRIM28 at serine 824 (p-TRIM28, S824). This modification converts TRIM28 from a transcriptional inhibitor to a transactivator for HIV transcription. Additionally, we observed that the phosphorylation of TRIM28 (p-TRIM28, S824) promotes the transition from the pausing phase to the elongation phase of HIV transcription, thereby facilitating the production of full-length HIV genomic transcripts. This finding corroborates the previously observed enhanced RNAP II CTD phosphorylation at Ser2, a marker of transcriptional elongation, following cocaine exposure. Accordingly, upon cocaine treatment, we observed the elevated recruitment of p-TRIM28-(S824) at the HIV LTR. Overall, our results unravel the intricate molecular mechanisms underlying cocaine-induced HIV transcription and gene expression. These findings hold promise for the development of highly targeted therapeutics aimed at mitigating the detrimental effects of cocaine in individuals living with HIV.
Role of Oxidative Stress and Inflammation in Postoperative Complications and Quality of Life After Laryngeal Cancer Surgery
Authors: Zivkovic Andjela , Jotic Ana , Dozic Ivan , Randjelovic Simona , Cirkovic Ivana , Medic Branislava , Milovanovic Jovica , Trivić Aleksandar , Korugic Aleksa , Vukasinović Ivan , Savic Vujovic Katarina
Published: November 2024

(1) Background: Laryngeal surgery due to carcinoma leads to significant tissue disruption, cellular injury, and inflammation. This leads to increased levels of reactive oxygen species (ROS), causing oxidative damage that can influence quality of life (QOL) and recovery and complicate the postoperative course. The aim of this study was to compare how postoperative quality of life and surgical complication occurrence interacted with the biomarker levels of oxidative stress (malondialdehyde, MDA; superoxide dismutase, SOD; glutathione peroxidase 1, GPX1; and catalase, CAT) and inflammation (interleukin 1, IL-1; interleukin 6, IL-6; C-reactive protein, CRP) in patients treated with conservative and radical laryngeal surgery. (2) Methods: The study included 56 patients who underwent surgical treatment for laryngeal cancer. Blood samples were collected to analyze oxidative stress and inflammation parameters before surgery and on the first and seventh days postoperatively. Serum concentrations of MDA, SOD, GPX, CAT, IL-1, IL-6, and CRP were measured using coated enzyme-linked immunosorbent assay (ELISA) kits. EORTC QLQ-H&H43 questionnaire was used to measure the QOL of patients. (3) Results and Conclusions: T stage, pain intensity, and the extent of the surgical procedure were established as significant predictive factors for QOL in multivariate analysis. There was a significant positive correlation between surgical complication occurrence and preoperative values of GPX and MDA, but significant predictors of surgical complication occurrence on the 7th postoperative day were SOD and MDA values (p < 0.05).
Overexpression of Egr1 Transcription Regulator Contributes to Schwann Cell Differentiation Defects in Neural Crest-Specific Adar1 Knockout Mice
Authors: Zerad Lisa , Gacem Nadjet , Gayda Fanny , Day Lucie , Sinigaglia Ketty , Richard Laurence , Parisot Melanie , Cagnard Nicolas , Mathis Stephane , Bole-Feysot Christine , O’Connell Mary A. , Pingault Veronique , Dambroise Emilie , Keegan Liam P. , Vallat Jean Michel , Bondurand Nadege
Published: November 2024

Adenosine deaminase acting on RNA 1 (ADAR1) is the principal enzyme for the adenosine-to-inosine RNA editing that prevents the aberrant activation of cytosolic nucleic acid sensors by endogenous double stranded RNAs and the activation of interferon-stimulated genes. In mice, the conditional neural crest deletion of Adar1 reduces the survival of melanocytes and alters the differentiation of Schwann cells that fail to myelinate nerve fibers in the peripheral nervous system. These myelination defects are partially rescued upon the concomitant removal of the Mda5 antiviral dsRNA sensor in vitro, suggesting implication of the Mda5/Mavs pathway and downstream effectors in the genesis of Adar1 mutant phenotypes. By analyzing RNA-Seq data from the sciatic nerves of mouse pups after conditional neural crest deletion of Adar1 (Adar1cKO), we here identified the transcription factors deregulated in Adar1cKO mutants compared to the controls. Through Adar1;Mavs and Adar1cKO;Egr1 double-mutant mouse rescue analyses, we then highlighted that the aberrant activation of the Mavs adapter protein and overexpression of the early growth response 1 (EGR1) transcription factor contribute to the Adar1 deletion associated defects in Schwann cell development in vivo. In silico and in vitro gene regulation studies additionally suggested that EGR1 might mediate this inhibitory effect through the aberrant regulation of EGR2-regulated myelin genes. We thus demonstrate the role of the Mda5/Mavs pathway, but also that of the Schwann cell transcription factors in Adar1-associated peripheral myelination defects.
The Formation of Human Arteriovenous Malformation Organoids and Their Characteristics
Authors: Oh Eun Jung , Kim Hyun Mi , Kwak Suin , Huh Chanhoe , Chung Ho Yun
Published: November 2024

Arteriovenous malformations (AVMs) are characterized by direct connections between arteries and veins without intervening capillaries, with the concomitant formation of abnormal vascular networks associated with angiogenesis. However, the current understanding of the diagnosis and treatment of AVMs is limited, and no in vitro disease models exist at present for studying this condition. In this study, we produced endothelial cells (ECs) in two-dimensional cultures and three-dimensional (3D) blood vessel organoids (BVOs), comparing gene expression profiles between normal and AVM organoids. The normal and AVM organoids were examined via immunofluorescence staining using CD31 and phalloidin. The AVM organoids showed significantly higher expression levels of CD31 and phalloidin than the normal organoids. Genes such as FSTL1, associated with angiogenesis, showed significantly higher expression in the AVM organoids than in the normal organoids. In contrast, the MARCKS gene exhibited no significant difference in expression between the two types of organoids. The capillaries and related CSPG4 genes exhibited the lowest expression in the 3D AVM organoids. Furthermore, hsa-mir-135b-5p, a small RNA related to AVMs, showed elevated expression in AVM tissues and significantly higher levels in 3D AVM organoids. In our study, we were able to successfully establish AVM organoids (hBVOs) containing ECs and mural cells through advancements in stem cell and tissue engineering. These organoids serve as valuable models for investigating disease mechanisms, drug development, and screening potential therapeutic interventions in drug discovery. These findings contribute essential insights for the development of treatment strategies targeting AVMs.
Development of All-Solid-State Potentiometric Sensors for Monitoring Carbendazim Residues in Oranges: A Degradation Kinetics Investigation
Authors: Hassan Yasmeen A. A. , Okeil Sherif , Ayad Miriam F. , Hussein Lobna A. , Fares Nermine V.
Published: November 2024

Monitoring fungicide residues in orange fruits is vital, as fungicides for orange cultivation are increasingly used to prevent yield loss. At the same time, increasing restrictions are added by regulatory organizations. For facile on-site monitoring of the fungicide carbendazim (MBC), five ion-selective potentiometric sensors are proposed and compared. The first two sensors were prepared with a precipitation-based technique using molybdate (sensor 1) and tetraphenylborate (TPB) (sensor 2), respectively. Furthermore, two ionophore-based sensors were prepared using β-cyclodextrin as ionophore together with TPB (sensor 3) and tetrakis(4-chlorophenyl)borate (TpClPB) (sensor 4) as ion-exchanger. Further incorporation of multi-walled carbon nanotubes (MWCNTs) between the graphite rod and the sensing membrane of sensor 4 (sensor 5) further improved the stability and significantly lowered the limit of detection (LOD). Their performance was evaluated according to IUPAC recommendations, revealing linear response in the concentration range 1 × 10−4–1 × 10−2 M, 1 × 10−5–1 × 10−2 M, 1 × 10−5–1 × 10−3 M, 1 × 10−6–1 × 10−3 M, and 1 × 10−7–1 × 10−3 M with a Nernstian slope of 54.56, 55.48, 56.00, 56.85, and 57.34 mV/decade, respectively. The LOD values for the five sensors were found to be 7.92 × 10−5, 9.98 × 10−6, 9.72 × 10−6, 9.61 × 10−7, and 9.57 × 10−8 M, respectively. The developed potentiometric sensors were successfully applied to determine the residue and degradation rate of MBC in orange samples. After the researched fungicide was applied to the orange trees, the preharvest interval (PHI) could be calculated based on the MBC degradation kinetics determined in the tested orange samples.
Mapping Psychosocial Challenges, Mental Health Difficulties, and MHPSS Services for Unaccompanied Asylum-Seeking Children in Greece: Insights from Service Providers
Authors: Giannopoulou Ioanna , Papanastasatos Gerasimos , Vathakou Eugenia , Bellali Thalia , Tselepi Konstantia , Papadopoulos Paraskevas , Kazakou Myrsini , Papadatou Danai
Published: November 2024

Background/Objectives: Evidence-based information is crucial for policymakers and providers of mental health and psychosocial services (MHPSS) for unaccompanied asylum-seeking children (UASC). However, there is a scarcity of national-level studies investigating the MHPSS needs of UASC and how these are addressed in Greece. The research objectives of this study were to explore: (a) the psychosocial and mental health needs of UASC living in Greek long-term accommodation facilities as perceived by MHPSS providers, and (b) the range of services across the country, highlighting gaps and best practices in service delivery. Method: An exploratory, predominantly quantitative design was adopted to map UASC’s psychosocial difficulties, mental health problems, and MHPSS delivery. Purposive sampling was implemented, with 16 of 17 NGOs operating long-term accommodation facilities for UASC and 16 child and adolescent mental health services (CAMHS) participating. The sample included 79 participants (34 facility coordinators, 28 field psychologists, and 16 CAMHS directors). A 5-W mapping tool (Who, Where, What, When, and Which) was used for data collection, through an online survey. Data analysis involved quantitative and qualitative methods (content analysis). Results: Of 798 minors, almost 59% showed signs of behavioral or emotional disturbance, with over half referred for psychiatric assessment and 27.7% needing inpatient care. Aggression, disruptive behaviors, self-harm, and suicidal ideation were the most challenging issues. CAMHS directors reported a high rate of crisis-driven responses, with 42.1% of UASC needing emergency psychiatric evaluation. Psychosocial support was hindered by communication difficulties, lack of a shared care philosophy, understaffing, job insecurity, and limited resources. Conclusions: Our findings highlight the mental health needs of UASC, and the challenges faced by facility coordinators, psychologists, and community mental health specialists. Future research should focus on the institutional and organizational factors influencing service delivery to improve support for UASC.
Novel MSX1 Gene Variants in Chinese Children with Non-Syndromic Tooth Agenesis: A Clinical and Genetic Analysis
Authors: Ding Tingting , Liu Haochen , Yu Guoxia
Published: November 2024

Background: Tooth agenesis is the most frequently occurring genetic developmental anomaly in clinical dentistry. The MSX1 gene, essential for tooth development, has been associated with non-syndromic tooth agenesis. This study aims to identify novel MSX1 variants associated with this condition and to understand their impact on tooth development. Methods: This study involved the genetic analysis of two children presenting with non-syndromic tooth agenesis. Conservation analysis and 3D structural modeling were conducted to assess the pathogenicity of these variants. Additionally, a review of 108 patients with known MSX1 variants was performed to identify patterns of tooth agenesis. Results: We discovered two novel MSX1 variants, c.823 T>G and c.890 A>G, located in the second exon of the MSX1 gene. The identified MSX1 variants, c.823 T>G and c.890 A>G, were predicted to be pathogenic. Conservation analysis showed that the impacted amino acids are highly conserved across species, and 3D structural analysis indicated potential disruptions to protein function. Among the 108 patients reviewed, a consistent pattern of tooth agenesis was observed, with the most frequently missing teeth being the maxillary second premolars, the mandibular second premolars, and the maxillary first premolars. Conclusions: This research broadens the known range of MSX1 gene variants and deepens our comprehension of the genetic foundations of non-syndromic tooth agenesis. The findings provide valuable insights for genetic counseling and future research into tooth development, emphasizing the importance of MSX1 in dental anomalies.
Combining Multi-Source Satellite Data with a Microclimate Model to Analyze the Microclimate of an Urban Park
Authors: Pan Yi , Morimoto Takehiro , Ichinose Toshiaki
Published: November 2024

Cities concentrate many people, and studies have shown that resultant urban heat islands can be intense. Urban parks can function as “cool islands” that mitigate heat island effects. This study used the microclimate model ENVI-met 5.1 to assess the cooling effect of Panyu Park in the center of Shanghai, China. The primary objectives were to increase the diversity of data sources and to conduct a microclimate analysis. Two scenarios were examined: the actual park and no park. The results indicated that (1) the integration of satellite technology enhanced the data sources for ENVI-met and thereby increased the efficiency of urban modeling and (2) the simulated results for the park correlated well with the actual data observed at weather stations. The presence of the park resulted in a decrease in the maximum air temperature by 0.1 °C at 1.4 m above ground, a decrease in the wind speed by 1.67 m/s, a maximum increase of 0.2% in relative humidity, and a reduction of 1.94 in the Predicted Mean Vote. The results demonstrated the applicability of multi-source satellite data in microclimate research, saved time on data collection, and provided valuable information for studies undertaken in areas where the collection of field data is challenging and/or historical data are unavailable.
Comparative Assessment of Aromatic Iron Corrosion Inhibitors with Electrochemical Methods
Authors: Roscher Jessica , Liu Dan , Xie Xuan , Holze Rudolf
Published: November 2024

Molecular aromatic corrosion inhibitors are frequently applied to slow down the corrosion of iron, its alloys and numerous other metallic materials. This case study with three representative aromatic inhibitors and a pure iron electrode aims at the verification of the reported conclusions regarding these inhibitors and at the verification and comparison of electrochemical corrosion assessment methods with attention to differences between iron alloys (steels) and pure iron possibly related to the presence/absence of alloying elements and non-iron impurities.
Refinement Properties and Refinement Mechanism of a New Master Alloy Al-5Ti-1B-1RE Refiner
Authors: Wang Zhengjun , Wang Shanmin , Yang Quanquan , Liu Xinyang , Dong Chen , Liu Lianxiang
Published: November 2024

To obtain high-quality grain refiner, a new Al-5Ti-1B-1RE master alloy grain refiner was synthesized by the melt-matching method. Its microstructure and refining effect, refining properties, and refining mechanism were analyzed. The experimental results show that the second-phase particles of Al-5Ti-1B-1RE master alloy are mainly TiB2, Al3Ti, Ti2Al20RE, etc. The magnitude of the free energy ΔG of the synthesis reaction is calculated to be ΔGTiB2 < ΔGAl3Ti < ΔGTi2Al20RE. The nucleation rate N mainly depends on the kinetic atomic diffusion activation energy Q and the thermodynamic nucleation work. The microstructure of commercial pure aluminum refined by the new grain refiner has almost transformed from coarse columnar crystals to fine equiaxed crystals, with an average grain size of 70.2 μm, which was 36.18% and 20.66% smaller than that refined by domestic and imported Al-Ti-B wire master alloy grain refiner, its mechanical properties of tensile strength σb were increased by 11.94% and 8.29%, and elongation δ was improved by 31.79% and 17.41%, respectively. The main refinement mechanism is the formation of TiAl3 on TiB2 particles and the release of RE atoms from the Ti2Al20RE phase, which in turn is partially transformed into the TiAl3 phase, which promotes dual nucleation refinement.
Predicting Dynamic Properties and Fatigue Performance of Aged and Regenerated Asphalt Using Time–Temperature–Aging and Time–Temperature–Regenerator Superposition Principles
Authors: Wang Zhaoli , Ding Hongli , Ma Xiaoyan , Yang Wanhong , Ma Xiaojun
Published: November 2024

Reclaimed asphalt pavement (RAP) reduces energy consumption and enhances economic benefits by recycling road materials, making it an effective approach for the sustainable use of solid waste resources. The performance of reclaimed asphalt pavement is significantly affected not only by the degradation of asphalt binders due to aging but also by the dosage of the rejuvenator used. The master curve of the complex shear modulus is widely recognized as a valuable tool for characterizing the rheological properties of asphalt binders. First, a virgin asphalt binder with a grade of SK70 was subjected to varying degrees of aging, followed by the rejuvenation of the aged asphalt using different dosages of the rejuvenator. Second, frequency sweeps were conducted on the aged and rejuvenated asphalt binders at various temperatures. Complex modulus master curves were constructed, and the CAM model was applied to fit these curves. The viscoelastic properties of asphalt at different aging levels and rejuvenator dosages were then analyzed based on the CAM parameters. Next, by applying a curve-shifting technique based on the least squares method to a reference state, both the time–temperature–aging (TTA) and time–temperature–regenerator (TTR) master curves of the complex modulus were constructed. The relationships between aging shift factors and aging times, as well as between regenerator shift factors and dosages, were established to predict the complex moduli of both aged and rejuvenated asphalt. Finally, the shear stress–strain relationships and material integrity of aged and rejuvenated asphalt were evaluated to assess their fatigue performance. The results indicated that aging significantly increases the complex modulus of asphalt, with TFOT (Thin Film Oven Test) aging having a more pronounced impact than PAV (Pressurized Aging Vessel) aging, resulting in reduced viscous deformation and an increased risk of cracking. Rejuvenator dosage reduces the complex modulus, with a 6% dosage effectively restoring mechanical properties and enhancing low-temperature performance. The TTA master curve demonstrates a strong linear correlation between aging shift factors and time, allowing for accurate predictions of the complex modulus of aged asphalt. Similarly, the TTR master curve reveals a linear relationship between regenerator dosage and shift factor, offering high predictive accuracy for optimizing regenerator dosages in engineering applications. The study further explores how varying levels of aging and rejuvenator dosage affect fatigue life under different strain conditions, uncovering complex behaviors influenced by these aging and regeneration processes.
Sustainable Enzymatic Production of Bioactive Compounds: From Designing to Bioavailability
Authors: Neves Raquel , Ribeiro Maria H. L.
Published: November 2024

This study evaluates the feasibility and effectiveness of using immobilized pectinase enzymes for juice processing to reduce cloudiness while preserving nutritional and bioactive properties. The research is driven by the increasing demand for innovative food products that offer enhanced functionality and health benefits. It focuses on the development and application of immobilized biocatalysts in bioprocessing, specifically using pectinase encapsulated in a sol–gel matrix. Reaction parameters for the interaction between immobilized pectinase and its primary substrate, pectin, were optimized through systematic experimentation. Optimal conditions were established, achieving enhanced enzyme activity and stability with 0.15 g of lens-shaped capsules containing 10.0 mg/mL pectinase in 24-well microplates as microreactors. Kinetic studies indicated improved substrate affinity after immobilization (Km = 0.115 mg/mL), particularly when magnetized (Kmi = 0.041 mg/mL). Operational stability and reusability assessments demonstrated potential for extended use with magnetized pectinase capsules retaining higher residual activity after a fourth reuse cycle (155% > 75%). The application of immobilized pectinase in processing peach nectar successfully reduced cloudiness and increased the release of bioactive compounds, enhancing antioxidant and anti-inflammatory activities, as evaluated by the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and the albumin method, respectively. In vitro digestion studies revealed dynamic activity profile changes, highlighting the impact of juice bioprocessing on bioavailability.
A Review of Non-Functional Requirements Analysis Throughout the SDLC
Authors: Dongmo Cyrille
Published: November 2024

To date, unquestionable efforts have been made, both in academia and industry, to facilitate the development of functional requirements (FRs) throughout the different phases of the software development life cycle (SDLC). Functional requirements are understood to mean the users’ needs pertaining to the services to be rendered by a software system. For example, semi-formal or graphically based approaches such as UML, and mathematically based or formal approaches such as Z and related tools have all been developed with the intention of addressing FRs. In the same vein, most of the proposed software methodologies, for instance, agile software development and model-driven software development, primarily target functional requirements. Considering the importance and even the criticality of non-functional requirements describing the quality of software systems and the constraints upon them, similar progress would be expected for their development. However, it appears that making headway with NFRs has been more challenging due to the complexity of the requirements. In this regard, the main purpose of this work is to unveil (from the academic perspective) the current state of development of NFRs through the review of publications carefully selected from five online databases.
Atrial Fibrillation Type Classification by a Convolutional Neural Network Using Contrast-Enhanced Computed Tomography Images
Authors: Kotani Hina , Teramoto Atsushi , Ohno Tomoyuki , Sobue Yoshihiro , Watanabe Eiichi , Fujita Hiroshi
Published: November 2024

Catheter ablation therapy, which is a treatment for atrial fibrillation (AF), has a higher recurrence rate as AF duration increases. Compared to paroxysmal AF (PAF), sustained AF is known to cause progressive anatomic remodeling of the left atrium, resulting in enlargement and shape changes. In this study, we used contrast-enhanced computed tomography (CT) to classify atrial fibrillation (AF) into paroxysmal atrial fibrillation (PAF) and long-term persistent atrial fibrillation (LSAF), which have particularly different recurrence rates after catheter ablation. Contrast-enhanced CT images of 30 patients with PAF and 30 patients with LSAF were input into six pretrained convolutional neural networks (CNNs) for the binary classification of PAF and LSAF. In this study, we propose a method that can recognize information regarding the body axis direction of the left atrium by inputting five slices near the left atrium. The classification was visualized by obtaining a saliency map based on score-class activation mapping (CAM). Furthermore, we surveyed cardiologists regarding the classification of AF types, and the results of the CNN classification were compared with the results of physicians’ clinical judgment. The proposed method achieved the highest correct classification rate (81.7%). In particular, models with shallow layers, such as VGGNet and ResNet, are able to capture the overall characteristics of the image and therefore are likely to be suitable for focusing on the left atrium. In many cases, patients with an enlarged left atrium tended to have long-lasting AF, confirming the validity of the proposed method. The results of the saliency map and survey of physicians’ basis for judgment showed that many patients tended to focus on the shape of the left atrium in both classifications, suggesting that this method can classify atrial fibrillation more accurately than physicians, similar to the judgment criteria of physicians.
The Electron–Phonon Interaction in Non-Stoichiometric Bi2Sr2CaCu2O8+δ Superconductor Obtained from the Diffuse Elastic Scattering of Helium Atoms
Authors: Benedek Giorgio , Manson Joseph R. , Miret-Artés Salvador , Schmicker Detlef , Toennies Jan Peter
Published: November 2024

Previously, helium atom scattering (HAS) has been shown to probe the electron–phonon interaction at conducting crystal surfaces via the temperature dependence of the specular peak intensity. This method is now extended to non-stoichiometric superconductors. The electron–phonon interaction, as expressed by the mass-enhancement factor λ, is derived from the temperature dependence of the diffuse elastic scattering intensity, which specifically depends on the non-stoichiometric component responsible for superconductivity. The measured value of the mass-enhancement factor for Bi2Sr2CaCu2O8+δ at the optimal doping δ = 0.16 is λ = 0.55 ± 0.08 is in good agreement with values of λ recently estimated with other methods. This also confirms the relevant role of electron–phonon interaction in high-temperature non-stoichiometric cuprate superconductors.
Retinol and Hydroxyasiaticoside Synergistically Relieve Histamine-Induced Atopic Dermatitis Activity by Repressing TRPV1, L1R1, and CD130 Targets
Authors: Liu Qin , Shu Peng , Song Qingle , Huang Zhenhai , Weng Jiyu , Zhang Lanyue , Wang Yuan , Liu Qingqing
Published: November 2024

Background: Retinol, an important bioactive substance with multiple physiological functions such as promoting collagen synthesis, inhibiting matrix metalloproteinase activity, alleviating oxidative stress, regulating gene expression, and promoting epidermal cell proliferation, has a significant effect on skin damage recovery. Hydroxyasiaticoside, a triterpenoid saponin derived from Centella asiatica (L.) Urb., is closely related to the secretion of collagen types I and III, and possesses multiple biological activities, including moisturizing, antioxidants, anti-apoptosis, neuroprotection, anti-inflammation, and the promotion of wound healing. It plays a particularly prominent role in reducing oxidative stress in wounds and inducing vasodilatation. Objective: The aim of this study was to investigate the therapeutic efficacy of retinol combined with hydroxyasiaticoside in histamine-induced atopic dermatitis. Materials and Methods: The experiment was carried out using three different concentrations of a retinol and hydroxyasiaticoside mixed solution: low, medium, and high concentrations. After inducing atopic dermatitis in mice through histamine administration, these solutions were applied to the skin surface of the mice, and a comparative analysis was conducted with both the control group and the model group. The effect of combination therapy on atopic dermatitis was evaluated through histopathology, immunohistochemistry, and transcriptomic analysis. Results: The combination of retinol and hydroxyasiaticoside significantly attenuated histamine-induced scratching behaviors, alleviated the phenomenon of epidermal hyperplasia, and effectively reduced the proliferation, infiltration, and degranulation of mast cells. In addition, the combination inhibited the expression of relevant pro-inflammatory cytokines. Quantitative RNA-seq analysis revealed that the gene expression patterns were similar in different concentration groups. However, the medium dose group may be able to regulate skin inflammation by regulating upstream genes to inhibit autophagy-related pathways. Further GO analysis revealed that the low-dose group mainly affected metabolism-related genes, the medium-dose group affected more genes related to body systems, and the high-dose group was dominated by genes related to human diseases.
Tellurium Photonic Crystal-Based Terahertz Polarization Splitter Using a Diamond-Shaped Ferrite Pillar Array
Authors: Zhang Haiping , Zeng Zhifeng , Wang Yong
Published: November 2024

A T-shaped photonic crystal waveguide was designed with square lattice tellurium photonic crystals. A diamond-shaped ferrite pillar array was inserted in the junction of the waveguide to make a novel terahertz polarization splitter. Both transverse electric and transverse magnetic modes were numerically investigated by the plane wave expansion method, which used complete photonic band gaps covering from 0.138 THz to 0.144 THz. In this frequency domain of the fully polarized band gaps, the transmission efficiency of the photonic crystal waveguide was up to −0.21 dB and −1.67 dB for the transverse electric and transverse magnetic modes, respectively. Under the action of a DC magnetic field, the THz waves were rotated 90 degrees by the diamond-shaped ferrite pillar array. Transverse electric waves or transverse magnetic waves can be separated by a polarization isolator (six smaller tellurium rods) from the fixed waves. The characteristics of the designed polarization splitter were analyzed by the finite element method, and its transmission efficiency was optimized to 95 percent by fine-tuning the radii of the thirteen ferrite pillars. A future integrated communication network of sky–earth–space will require fully polarized devices in the millimeter and terahertz wavebands. The envisaged polarization splitter has a unique function and provides a promising method for the realization of fully polarized 6G devices.
The New Interpretation of the Photothermal Spectra of CdTe Samples After Different Surface Treatments
Authors: Zakrzewski Jacek , Maliński Mirosław , Boumhamdi Mohammed , Strzelecki Janusz , Strzałkowski Karol
Published: November 2024

This article presents new research on the surface condition of bulk crystal samples after the following stages of surface treatment: grinding, polishing, and etching. Furthermore, it shows how the surface condition affects the photothermal signal’s spectral amplitude and phase characteristics (PZE). A new theoretical interpretation of the photothermal spectra of CdTe samples after different surface treatments is proposed. We demonstrate that the piezoelectric method is susceptible to the surface condition, and it allows for the estimation of the thickness of surface-damaged layers of samples, and for the analysis of their thermal parameters. The roughness of surfaces obtained from the AFM pictures is estimated and compared to the photothermal results.