bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of novel autoantigens as potential biomarkers in juvenile idiopathic arthritis associated uveitis
Authors: Arve-Butler Sabine , Mossberg Anki , Kahn Fredrik , Najibi Seyed Morteza , Berthold Elisabet , Król Petra , Månsson Bengt , Kahn Robin
Published: January 2023

Background

Many children with juvenile idiopathic arthritis (JIA) have autoantibodies, targeting nuclear components (anti-nuclear antibodies, ANA). ANA in JIA is associated with uveitis, an eye inflammation which may cause permanent vision impairment if not detected and treated. However, ANA-testing is neither specific nor sensitive enough to be a clinically reliable predictor of uveitis risk, and the precise autoantigens targeted by ANA in JIA are largely unknown. If identified, specific autoantibodies highly associated with uveitis could be used as biomarkers to facilitate identification of JIA patients at risk.

Methods

Antibodies from six ANA-positive, oligoarticular JIA patients, with and without uveitis, were explored by two large-scale methods: (1) screening against 42,100 peptides on an autoimmunity profiling planar array, and (2) immunoprecipitations from cell lysates with antigen identification by mass spectrometry. Three hundred thirty-five peptide antigens, selected from proteins identified in the large-scale methods and the scientific literature were investigated using a bead-based array in a cohort of 56 patients with oligoarticular- or RF-negative polyarticular JIA, eight of which were having current or previous uveitis.

Results

In the planar array, reactivity was detected against 332 peptide antigens. The immunoprecipitations identified reactivity towards 131 proteins. Only two proteins were identified by both methods. In the bead-based array of selected peptide antigens, patients with uveitis had a generally higher autoreactivity, seen as higher median fluorescence intensity (MFI) across all antigens, compared to patients without uveitis. Reactivity towards 17 specific antigens was significantly higher in patients with uveitis compared to patients without uveitis. Hierarchical clustering revealed that patients with uveitis clustered together.

Conclusion

This study investigated autoantigens in JIA and uveitis, by combining two exploratory methods and confirmation in a targeted array. JIA patients with current or a history of uveitis had significantly higher reactivity towards 17 autoantigens and a generally higher autoreactivity compared to JIA patients without uveitis. Hierarchical clustering suggests that a combination of certain autoantibodies, rather than reactivity towards one specific antigen, is associated with uveitis. Our analysis of autoantibodies associated with uveitis in JIA could be a starting point for identification of prognostic biomarkers useful in JIA clinical care.
Efficacy of eltrombopag with immunosuppressive therapy for children with acquired aplastic anemia
Authors: Zhao Yufei , Yang Wenrui , Zhao Xin , Hu Xiangrong , Hu Jing , Liu Xu , Li Jianping , Ye Lei , Xiong Youzhen , Yang Yang , Zhang Baohang , Li Xiaoxia , Yang Xiawan , Shi Yimeng , Peng Guangxin , Li Yuan , Fan Huihui , Zhou Kang , Jing Liping , Zhang Li , Zhang Fengkui
Published: January 2023

Background

Eltrombopag (EPAG), an oral thrombopoietin receptor agonist (TPO-RA), has been proven to improve the hematologic response without increasing toxic effects as a first-line therapy combined with standard immunosuppressive treatment (IST) in adults with severe aplastic anemia (SAA). Nevertheless, the clinical evidence on the efficacy of EPAG in children with acquired aplastic anemia is limited and controversial.

Methods

We performed a single-center, retrospective study to analyze the clinical outcomes of fifteen patients aged ≤18 years with newly diagnosed acquired SAA who received first-line IST and EPAG (EPAG group) compared with those of forty-five patients who received IST alone (IST group) by propensity score matching (PSM).

Results

There was no difference in the overall response (OR) rate between the EPAG group and IST group (53.3% vs. 46.7% at 3 months, P = 0.655; 66.7% vs. 57.8% at 6 months, P = 0.543), but the complete response (CR) rate was statistically significant (20.0% vs. 4.4% at 3 months, P = 0.094; 46.7% vs. 13.3% at 6 months, P = 0.012). The median time to achieve a hematological response in the EPAG and IST groups was 105 days and 184 days, respectively. No difference was observed in the event-free survival (EFS) or overall survival (OS) rates.

Conclusion

Adding EPAG to standard IST as the first-line treatment for children with acquired SAA improved the rapidity of hematological response and the CR rate but did not improve the OR or EFS rates.
Foreign bodies in children's lower urinary tract: A case series and literature review
Authors: Kuang Tongshuai , Cai Wei , Qian Weite , Lin Xiaokun
Published: January 2023

Background

Children with foreign bodies (FBs) in the lower urinary tract have rarely been reported, and their management remains challenging. This study aimed to describe the characteristics and treatment of FBs in children's lower urinary tract.

Methods

We retrospectively analyzed the clinical data on lower urinary tract FBs that were removed in our hospital from August 2017 to August 2022, including demographics, location, symptoms, imaging examinations, and treatment.

Results

Four male patients were enrolled, whose ages ranged from 9 to 13 years, with a mean age of 11 years. The course of the disease ranged from 3 h to 2 weeks. Their imaging characteristics were reviewed and analyzed, and two FBs were located in the bladder and two in the urethra. Mosquito forceps were used to remove an acne needle through the urethra in one case. Cystoscopy was first attempted in three cases, in only one of which was the FB removed successfully under endoscopic minimally invasive surgery. In the remaining two cases, removal via transurethral cystoscopy failed, whereby leading to cystotomy being performed. The FBs comprise a skipping rope, hairpin, magnetic bead, and acne needle. The postoperative recovery was uneventful, and no complications occurred during the follow-up period of 3 to 6 months.

Conclusion

It is rare for children to have FBs in the lower urinary tract. An early diagnosis, as well as appropriate management of lower urinary tract FBs, can significantly reduce complications. Surgical removal of lower urinary tract FBs can be safe and effective, and relatively better outcomes can be achieved.
Robot-assisted cannulated compression screw internal fixation for treatment of femoral neck fracture in children: A case series of ten patients
Authors: Feng Wei , Yao Ziming , Liu Haonan , Guo Dong , Zhu Danjiang , Song Baojian , Wang Qiang , Zhang Xuejun
Published: January 2023

Purpose

To investigate the safety and efficacy of robot-assisted cannulated compression screw internal fixation in the treatment of femoral neck fracture in children.

Methods

We retrospectively reviewed the data of ten children with femoral neck fractures treated by robot-assisted internal fixation from January 2020 to June 2021. The clinical and radiological characteristics, operation duration, and fluoroscopy frequency of robot-assisted screws placement together with the complications and function were evaluated. At the 12-month follow-up, the hip joint function was evaluated using the Ratliff classification.

Results

Ten children, six boys and four girls, aged 4–14 years were included. There were eight type II and three type III femoral neck fractures using the Delbet classification. In the process of robot-assisted internal fixation, the median of fluoroscopy frequency was 22 times and the median of operation duration was 47 min. The median of screw parallelism was 1.33° and 0.66° on the anteroposterior and lateral x-ray films, and the median of screw distribution was 41.86% and 44.93% on the anteroposterior and lateral x-ray films, respectively. At the 12-month follow-up, there were two cases of femoral head necrosis, and fracture healing was achieved in all patients, of which eight fractures were excellent and three were good by the Ratliff function classification.

Discussion

The application of robot-assisted cannulated compression screw internal fixation could help us achieve more safe and accurate screw placement, as well as a good treatment effect for children’s femoral neck fractures.

Level of Evidence

Level IV. retrospective case series.
Impact of 3D-printed models in meetings with parents of children undergoing interventional cardiac catheterisation
Authors: Karsenty Clément , Hadeed Khaled , Djeddai Camelia , Lateyron Julie , Guitarte Aitor , Vincent Remi , DeBarros Nathalie , Combes Nicolas , Briot Jerome , Dulac Yves , Yrondi Antoine , Acar Philippe
Published: January 2023

Background

Paediatric interventional catheterisation has consistently improved in recent decades, with often highly successful outcomes. However, progress is still required in terms of the information delivered to parents and how parental anxiety is managed.

Aim

To investigate the impact of cardiac printed models on improving parental understanding and alleviating anxiety before interventional catheterisation.

Methods

The parents of children undergoing interventional cardiac catheterisation were prospectively enrolled in the study. A questionnaire highlighting knowledge and understanding of the condition and cardiac catheterisation per se was scored on a scale of 1–30. The State-Trait Anxiety Inventory (STAI), which generates current anxiety scores, was also used before and after the pre-catheterisation meeting. The “printing group” received an explanation of catheterisation using the device and a three-dimensional (3D) model, while the “control group” received an explanation using only the device and a manual drawing.

Results

In total, 76 parents of 50 children were randomly assigned to a “control group” (n = 38) or “printing group” (n = 38). The groups were comparable at baseline. The level of understanding and knowledge improved after the “control group” and “printing group” meetings (+5.5±0.8 and +10.2±0.8; p < 0.0001 and p < 0.0001, respectively). A greater improvement was documented in the “printing group” compared to the “control group” (p < 0.0001). The STAI score also improved after the explanation was given to both groups (−1.8±0.6 and −5.6±1.0; p < 0.0001 and p < 0.0001). The greatest improvement was noted in the “printing group” (p = 0.0025). Most of the parents (35/38 from the “printing group”) found the models to be extremely useful.

Conclusion

3D-printed models improve parental knowledge and understanding of paediatric cardiac catheterisation, thereby reducing anxiety levels.
Risk factors of postoperative low cardiac output syndrome in children with congenital heart disease: A systematic review and meta-analysis
Authors: Wang Peiying , Fu Cangcang , Bai Guannan , Cuan Linbo , Tang Xiaomin , Jin Chendi , Jin Hongchong , Zhu Jihua , Xie Chunhong
Published: January 2023

Background

Low cardiac output syndrome (LCOS) is the most common complication after cardiac surgery, which is associated with the extension of postoperative hospital stay and postoperative death in children with congenital heart disease (CHD). Although there are some studies on the risk factors of LCOS in children with CHD, an unified conclusion is lack at present.

Purposes

To synthesize the risk factors of LCOS after CHD in children, and to provide evidence-based insights into the early identification and early intervention of LCOS.

Methods

The databases of the China National Knowledge Infrastructure (CNKI), Wanfang Database, China Science and Technology Journal Database (VIP), PubMed, Cochrane Library, Embase and Web of Science were searched for relevant articles that were published between the establishing time of each database and January 2022. Based on retrospective records or cohort studies, the influencing factors of postoperative low cardiac output in children with congenital heart disease were included in Meta analysis.This study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The risk of bias was evaluated according to the Newcastle-Ottawa Scale (NOS). RevMan 5.4 software was used to conduct the meta-analysis.

Results

A total of 1,886 records were screened, of which 18 were included in the final review. In total, 37 risk factors were identified in the systematic review. Meta- analysis showed that age, type of CHD, cardiac reoperation, biventricular shunt before operation, CPB duration, ACC duration, postoperative residual shunt, cTn-1 level 2 h after CPB > 14 ng/ml and postoperative 24 h MR-ProADM level > 1.5 nmol/l were independent risk factors of LCOS. Additionally, the level of blood oxygen saturation before the operation was found to have no statistically significant relationship with LOCS.

Conclusion

The risk factors of postoperative LCOS in children with CHD are related to disease condition, intraoperative time and postoperative related indexes, so early prevention should be aimed at high-risk children.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD42022323043.
Feasibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen self-testing in school and summer camp attendees
Authors: Colom-Cadena Andreu , Martínez-Riveros Héctor , Bordas Anna , Alonso-García Lucia , Montoro-Fernández Marcos , Romano-deGea Pol , Vidal-Alaball Josep , Solà-Segura Elisabet , Llibre Josep M. , Revollo Boris , Casabona Jordi , Agustí Cristina
Published: January 2023

Background

SARS-CoV-2 screening is one of the pillars of non-pharmaceutical preventive strategies to early identify and isolate infected individuals and therefore decrease community incidence.

Methods

We assessed the feasibility of severe acute respiratory syndrome coronavirus 2 self-testing with antigen-detecting rapid diagnostic tests in attendees of educational settings.

Results

A total of 305 students (88.15%) and 41 staff (11.85%) from 9 to 56 years old participated in the self-testing procedure and answered the survey at the end of the study. 91.3% (n = 313) did not need help, 96.1% of participants reported the same outcome as the healthcare workers. 94.5% strongly or slightly agree with the statement “I would repeat the experience”.

Conclusion

The study demonstrates that self-testing is acceptable and usable in children, adolescents and adults when the epidemiological situation may require a systematic screening of these populations, although supervision by health care or previously trained personnel is recommended for younger age groups.
Curcumin enhances the anti-cancer efficacy of paclitaxel in ovarian cancer by regulating the miR-9-5p/BRCA1 axis
Authors: Liu Yuwan , Shen Zhangjin , Zhu Tingjia , Lu Weiguo , Fu Yunfeng
Published: January 2023

Background: Patients with late-stage ovarian cancer still have a very poor prognosis due to chemotherapy resistance. Curcumin has been shown to synergistically enhance the therapeutic effects of multiple chemotherapeutic agents, but the potential involvement of curcumin in ovarian cancer is largely unknown. This study aimed to investigate whether curcumin has synergistic anti-cancer effects with paclitaxel in ovarian cancer and its underlying mechanism.

Methods: Ovarian cancer cell lines (SKOV3 and A2780) were treated with curcumin, alone or combined with paclitaxel. Cell viability, colony formation, EdU incorporation assays, and flow cytometry were used to assess cell proliferation, apoptosis, and cell cycle progression. The cytotoxic synergistic effect of curcumin and paclitaxel was detected by Calcusyn software. RNA immunoprecipitation assay was used to verify the interaction between miR-9-5p and BRCA1. qRT-PCR and Western blot were performed to detect gene and protein expression.

Results: We found that curcumin and paclitaxel synergistically inhibited proliferation and promoted apoptosis in ovarian cancer cells. Furthermore, curcumin and paclitaxel combination resulted in decreased miR-9-5p expression and increased BRCA1 expression. Functionally, miR-9-5p overexpression counteracted the synergistic effect of curcumin and paclitaxel on cell proliferation and apoptosis by targeting BRCA1. Meanwhile, in vivo experiments revealed that curcumin and paclitaxel combination dramatically suppressed the growth of transplanted tumors, while miR-9-5p mimics eliminated the growth inhibition of xenografts induced by the combined treatment.

Conclusion: Curcumin enhanced the anti-cancer efficacy of paclitaxel in ovarian cancer by regulating the miR-9-5p/BRCA1 axis. These findings provide strong evidence for clinical investigation of curcumin and paclitaxel combination as a novel strategy for ovarian cancer patients, and identify miR-9-5p and BRCA1 as key targets for regulating sensitivity to this therapy.
The pathological mechanism of the COVID-19 convalescence and its treatment with traditional Chinese medicine
Authors: Jiang Linlin , An Xuedong , Duan Yingying , Lian Fengmei , Jin De , Zhang Yuehong , Yang Cunqing , Zhang Yuqing , Kang Xiaomin , Sun Yuting
Published: January 2023

The severe acute respiratory syndrome coronavirus - 2 (SARS - CoV - 2) was reported to cause the Wuhan outbreak of the corona virus disease 2019(COVID-19). To date, the COVID-19 has infected more than 600 million people gloabally. As a growing number of patients recover from acute infections and are discharged from hospitals, the proportion of patients in the recovery period is gradually increasing. Many of these individuals have been reported to experience multiple symptoms during the convalescence, such as fatigue, dyspnea and pain which are designated as “long-COVID”, “post-COVID syndrome” or “recovery sequelae. We searched for recent articles published in PubMed on COVID-19 convalescence and found that the pathogenesis of COVID-19 convalescence is not yet well recognized. It may be associated with incomplete recovery of immune system, parenchymal organ damage (liver or lung), coagulation abnormalities, “second hit” caused by viral infection, and Phenomenon of Cell Senescence-Associated Secretory Phenotype (SASP). Some drugs and psychological factors of patients also play a non-negligible role in it. We also found that the effect of traditional Chinese medicine (TCM) is effective in the treatment of the COVID-19 recovery phase, which can not only relieve the corresponding symptoms, but also improve the indicators and pulmonary fibrosis. Bufei Huoxue Capsule, as the only drug explicitly mentioned for COVID-19 recovery period, can exert strong rehabilitative effects on physiological activity in patients recovering from COVID-19. In addition, in previous studies, traditional Chinese medicine has been confirmed to have the ability to resist cytokine storms, as well as improve coagulation and myocardial damage, which makes it have potential therapeutic advantages in targeting the hyperimmune response, coagulation abnormalities and myocardial damage existing in the recovery period. In conclusion, the clinical symptoms of patients convalescing from COVID-19 are complex, and its pathogenesis has not been elucidated. traditional Chinese medicine, as a traditional treatment, its specific action and mechanism need to be confirmed by more studies, so that it can play a better role.
Dobutamine promotes the clearance of erythrocytes from the brain to cervical lymph nodes after subarachnoid hemorrhage in mice
Authors: Wang Xue , Deng Hong-Ji , Gao Sheng-Qing , Li Tao , Gao Chao-Chao , Han Yan-Ling , Zhuang Yun-Song , Qiu Jia-Yin , Miao Shu-Hao , Zhou Meng-Liang
Published: January 2023

Background: Erythrocytes and their breakdown products in the subarachnoid space (SAS) are the main contributors to the pathogenesis of subarachnoid hemorrhage (SAH). Dobutamine is a potent β1-adrenoreceptor agonist that can increase cardiac output, thus improving blood perfusion and arterial pulsation in the brain. In this study, we investigated whether the administration of dobutamine promoted the clearance of red blood cells (RBCs) and their degraded products via meningeal lymphatic vessels (mLVs), thus alleviating neurological deficits in the early stage post-SAH.

Materials and methods: Experimental SAH was induced by injecting autologous arterial blood into the prechiasmatic cistern in male C57BL/6 mice. Evans blue was injected into the cisterna magna, and dobutamine was administered by inserting a femoral venous catheter. RBCs in the deep cervical lymphatic nodes (dCLNs) were evaluated by hematoxylin–eosin staining, and the hemoglobin content in dCLNs was detected by Drabkin’s reagent. The accumulation of RBCs in the dura mater was examined by immunofluorescence staining, neuronal death was evaluated by Nissl staining, and apoptotic cell death was evaluated by TUNEL staining. The Morris water maze test was used to examine the cognitive function of mice after SAH.

Results: RBCs appeared in dCLNs as early as 3 h post-SAH, and the hemoglobin in dCLNs peaked at 12 h after SAH. Dobutamine significantly promoted cerebrospinal fluid (CSF) drainage from the SAS to dCLNs and obviously reduced the RBC residue in mLVs, leading to a decrease in neuronal death and an improvement in cognitive function after SAH.

Conclusion: Dobutamine administration significantly promoted RBC drainage from cerebrospinal fluid in the SAS via mLVs into dCLNs, ultimately relieving neuronal death and improving cognitive function.
Neuregulin-1β increases glucose uptake and promotes GLUT4 translocation in palmitate-treated C2C12 myotubes by activating PI3K/AKT signaling pathway
Authors: Yu Meirong , Wu Shuang , Gong Chao , Chen Lianhua
Published: January 2023

Insulin resistance (IR) is a feature of type 2 diabetes (T2DM) accompanied by reduced glucose uptake and glucose transporter 4 (GLUT4) translocation by skeletal muscle. Neuregulin-1β (NRG-1β) is essential for myogenesis and the regulation of skeletal muscle metabolism. Neuregulin-1β increases insulin sensitivity, promotes glucose uptake and glucose translocation in normal skeletal muscle. Here, we explored whether Neuregulin-1β increased glucose uptake and GLUT4 translocation in palmitate (PA)-treated C2C12 myotubes. After C2C12 myoblasts differentiated into myotubes, we used palmitate to induce cellular insulin resistance. Cells were incubated with or without Neuregulin-1β and glucose uptake was determined using the 2-NBDG assay. The expression level of glucose transporter 4 (GLUT4) was measured via immunofluorescence and Western blotting. MK2206, an inhibitor of AKT, was employed to reveal the important role played by AKT signaling in PA-treated C2C12 myotubes. We then established an animal model with T2DM and evaluated the effects of Neuregulin-1β on body weight and the blood glucose level. The GLUT4 level in the gastrocnemius of T2DM mice was also measured. NRG-1β not only increased glucose uptake by PA-treated myotubes but also promoted GLUT4 translocation to the plasma membrane. The effect of NRG-1β on PA-treated C2C12 myotubes was associated with AKT activation. In T2DM mice, Neuregulin-1β not only improved diabetes-induced weight loss and diabetes-induced hyperglycemia, but also promoted GLUT4 translocation in the gastrocnemius. In summary, Neuregulin-1β increased glucose uptake and promoted translocation of GLUT4 to the plasma membrane in PA-treated C2C12 myotubes by activating the PI3K/AKT signaling pathway.
Induced pluripotent stem cells as natural biofactories for exosomes carrying miR-199b-5p in the treatment of spinal cord injury
Authors: Li Jun , Jing Yingli , Bai Fan , Wu Ying , Wang Limiao , Yan Yitong , Jia Yunxiao , Yu Yan , Jia Benzhi , Ali Fawad
Published: January 2023

Background: Induced pluripotent stem cells-derived exosomes (iPSCs-Exo) can effectively treat spinal cord injury (SCI) in mice. But the role of iPSCs-Exo in SCI mice and its molecular mechanisms remain unclear. This research intended to study the effects and molecular mechanism of iPSCs-Exo in SCI mice models.

Methods: The feature of iPSCs-Exo was determined by transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and western blot. The effects of iPSCs-Exo in the SCI mice model were evaluated by Basso Mouse Scale (BMS) scores and H&E staining. The roles of iPSCs-Exo and miR-199b-5p in LPS-treated BMDM were verified by immunofluorescence, RT-qPCR, and Cytokine assays. The target genes of miR-199b-5p were identified, and the function of miR-199b-5p and its target genes on LPS-treated BMDM was explored by recuse experiment.

Results: iPSCs-Exo improved motor function in SCI mice model in vivo, shifted the polarization from M1 macrophage to M2 phenotype, and regulated related inflammatory factors expression to accelerate the SCI recovery in LPS-treated BMDM in vitro. Meanwhile, miR-199b-5p was a functional player of iPSCs-Exo, which could target hepatocyte growth factor (Hgf). Moreover, miR-199b-5p overexpression polarized M1 macrophage into M2 phenotype and promoted neural regeneration in SCI. The rescue experiments confirmed that miR-199b-5p induced macrophage polarization and SCI recovery by regulating Hgf and Phosphoinositide 3-kinase (PI3K) signaling pathways.

Conclusion: The miR-199b-5p-bearing iPSCs-Exo might become an effective method to treat SCI.
Potentially inappropriate medication use and associated factors in residents of long-term care facilities: A nationwide cohort study
Authors: Jang Suhyun , Ah Young-Mi , Jang Sunmee , Kim Yeji , Lee Ju-Yeun , Kim Jung-Ha
Published: January 2023

Background: Residents in long-term care (LTC) facilities (LTCFs) may have multimorbidity and be unable to self-administer medication. Thus, due to the risk of potentially inappropriate medications (PIMs), epidemiological studies on PIM use and its associated factors should be conducted to ensure safe medication use for residents in LTCFs.

Objective: We evaluated PIM use among residents of LTCF and the associated factors in residents of LTCFs in Korea using a nationwide database.

Methods: This cross-sectional study used the Korea National Health Insurance Service Senior Cohort (KNHIS-SC) database 2.0 of the National Health Insurance Service (NHIS), a single public insurer in Korea. We analyzed older adults aged ≥65 years who were residents of LTCFs in 2018, using the KNHIS-SC database. The 2019 American Geriatrics Society (AGS) Beers criteria was used for PIM identification. The prevalence of PIM use was defined as the proportion of LTCF residents who received PIM prescriptions at least once. We evaluated the frequency of prescriptions, including PIMs, and determined the most frequently used PIMs. We also conducted a multivariable logistic regression analysis to identify the factors associated with PIM use.

Results: The prevalence of PIM among the LTCF residents was 81.6%. The prevalence of PIM was 74.9% for LTC grades 1 or 2 (high dependence) and 85.2% for LTC grades 3–5 (low dependence). Quetiapine was the most frequently prescribed PIM, followed by chlorpheniramine. The low dependence level was significantly associated with PIM use (odds ratio of LTC grades 3–5: 1.49, 95% confidence interval 1.32–1.68, reference: LTC grades 1 or 2); moreover, the number of medical institutions visited, and medications emerged as primary influencing factors.

Conclusion: Most LTCF residents were vulnerable to PIM exposure. Furthermore, exposure to PIMs is associated with LTC grade. This result highlights the need for comprehensive medication management of LTCF residents.
What do deep neural networks find in disordered structures of glasses?
Authors: Oyama Norihiro , Koyama Shihori , Kawasaki Takeshi
Published: January 2023

Glass transitions are widely observed in various types of soft matter systems. However, the physical mechanism of these transitions remains elusive despite years of ambitious research. In particular, an important unanswered question is whether the glass transition is accompanied by a divergence of the correlation lengths of the characteristic static structures. In this study, we develop a deep-neural-network-based method that is used to extract the characteristic local meso-structures solely from instantaneous particle configurations without any information about the dynamics. We first train a neural network to classify configurations of liquids and glasses correctly. Then, we obtain the characteristic structures by quantifying the grounds for the decisions made by the network using Gradient-weighted Class Activation Mapping (Grad-CAM). We consider two qualitatively different glass-forming binary systems, and through comparisons with several established structural indicators, we demonstrate that our system can be used to identify characteristic structures that depend on the details of the systems. Moreover, the extracted structures are remarkably correlated with the non-equilibrium aging dynamics in thermal fluctuations.
Influence of sweep interference on satellite navigation time-domain anti-jamming
Authors: Li Baiyu , Qiao Jia , Lu Zukun , Yu Xiaoyou , Song Jie , Lin Baojun , Li Xianghao
Published: January 2023

The global navigation satellite system (GNSS), represented by global positioning systems (GPS), is widely used in various civil and military fields and represents an essential basis for space-time information services. However, the radar signals partially overlap with the frequency band of satellite navigation signals, seriously affecting the normal reception of weak satellite navigation signal power. To further improve anti-jamming with sweep interference in the time domain, this paper focuses on the sweep interference scenario, studies the influence of the sweep interference on time-domain-adaptive anti-jamming, and proposes a timing reset based on the adaptive filter. The proposed method can effectively deal with the influence of sweep interference on time-domain-adaptive anti-jamming and can suppress interference and protect signals at the same time. Simulation experiments verify the effectiveness of the anti-jamming method proposed in this paper. Under the typical simulation scenarios, the influence time of the frequency sweep interference on the navigation signal is less than 1 m when the timing reset period is 1 m, which is significantly reduced compared to traditional methods. The proposed anti-jamming method is of great significance for improving the survivability of satellite navigation receivers in sweep interference scenarios.
Photonic crystal fiber (PhCF) for petrochemical sensing
Authors: Hossain Md. Bellal , Kříž Jan , Dhasarathan Vigneswaran , Rahaman Md. Ekhlasur
Published: January 2023

A square-core photonic crystal fiber (PhCF) has been presented for liquid (petrochemical) sensing application. The sensor performance has been studied numerically in the frequency range from 1.2 to 3.8 THz. The core hole is filled with pure petrol, kerosene, and diesel. Strut size is varied to explore the sensor’s performance and the analysis is performed in terahertz (THz) frequencies. The photonic crystal fiber sensor shows a relative sensitivity of around 97.0% and a confinement loss in the order of 10−14 at 3.7 THz. This photonic crystal fiber sensor can be fabricated using extrusion and 3D-printing technologies.
Dynamic analysis and optimal control of worm propagation model with saturated incidence rate
Authors: Wang Ruiling , Xue Yakui , Xue Kailin
Published: January 2023

In order to prevent the propagation of computer worms effectively, based on the latent character of worms, the exposed compartments of computer and USB device are introduced respectively, and a series of computer worm propagation models with saturation incidence rate are proposed. The qualitative behavior of the proposed model is studied. Firstly, the threshold R0 of the model is derived by using the next-generation matrix method, which completely characterized the stability of disease free equilibrium and endemic equilibrium. If R0 < 1, the disease free equilibrium is asymptotically stable, implying that the worm dies out eventually and its attack remains under control; if R0 > 1, the asymptotic stability of endemic equilibrium under certain conditions is proved, which means that the worm is always persistent and uncontrollable under such conditions. Secondly, the theoretical results are verified by numerical study, in which the relative importance of each parameter in worm prevalence is evaluated by sensitivity analysis. Finally, so as to minimize the number of computer and USB device carrying computer worms in short span of time, the worm propagation model is extended to incorporate three control strategies. The Pontryagin’s maximum principle is used to characterize the controls’ optimal levels. According to the control effect diagram, the combined strategy is effective in minimizing the transmission dynamics of worm virus in computer and USB devices populations respectively.
Exaggerated exercise pressor reflex in male UC Davis type 2 diabetic rats is due to the pathophysiology of the disease and not aging
Authors: Huo Yu , Grotle Ann-Katrin , McCuller Richard K. , Samora Milena , Stanhope Kimber L. , Havel Peter J. , Harrison Michelle L. , Stone Audrey J.
Published: January 2023

Introduction: Studies in humans and animals have found that type 2 diabetes mellitus (T2DM) exaggerates the blood pressure (BP) response to exercise, which increases the risk of adverse cardiovascular events such as heart attack and stroke. T2DM is a chronic disease that, without appropriate management, progresses in severity as individuals grow older. Thus, it is possible that aging may also exaggerate the BP response to exercise. Therefore, the purpose of the current study was to determine the effect of the pathophysiology of T2DM on the exercise pressor reflex independent of aging.

Methods: We compared changes in peak pressor (mean arterial pressure; ΔMAP), BP index (ΔBPi), heart rate (ΔHR), and HR index (ΔHRi) responses to static contraction, intermittent contraction, and tendon stretch in UCD-T2DM rats to those of healthy, age-matched Sprague Dawley rats at three different stages of the disease.

Results: We found that the ΔMAP, ΔBPi, ΔHR, and ΔHRi responses to static contraction were significantly higher in T2DM rats (ΔMAP: 29 ± 4 mmHg; ΔBPi: 588 ± 51 mmHg•s; ΔHR: 22 ± 5 bpm; ΔHRi: 478 ± 45 bpm•s) compared to controls (ΔMAP: 10 ± 1 mmHg, p < 0.0001; ΔBPi: 121 ± 19 mmHg•s, p < 0.0001; ΔHR: 5 ± 2 bpm, p = 0.01; ΔHRi: 92 ± 19 bpm•s, p < 0.0001) shortly after diabetes onset. Likewise, the ΔMAP, ΔBPi, and ΔHRi to tendon stretch were significantly higher in T2DM rats (ΔMAP: 33 ± 7 mmHg; ΔBPi: 697 ± 70 mmHg•s; ΔHRi: 496 ± 51 bpm•s) compared to controls (ΔMAP: 12 ± 5 mmHg, p = 0.002; ΔBPi: 186 ± 30 mmHg•s, p < 0.0001; ΔHRi: 144 ± 33 bpm•s, p < 0.0001) shortly after diabetes onset. The ΔBPi and ΔHRi, but not ΔMAP, to intermittent contraction was significantly higher in T2DM rats (ΔBPi: 543 ± 42 mmHg•s; ΔHRi: 453 ± 53 bpm•s) compared to controls (ΔBPi: 140 ± 16 mmHg•s, p < 0.0001; ΔHRi: 108 ± 22 bpm•s, p = 0.0002) shortly after diabetes onset.

Discussion: Our findings suggest that the exaggerated exercise pressor reflex and mechanoreflex seen in T2DM are due to the pathophysiology of the disease and not aging.
Effects of beetroot juice intake on repeated performance of competitive swimmers
Authors: Moreno Berta , Morencos Esther , Vicente-Campos Davinia , Muñoz Alejandro , González-García Jaime , Veiga Santiago
Published: January 2023

Background: Beetroot juice is a sport supplement with a high level of evidence on the physical performance enhancement. However, in swimming, there is no clear data about the effects of beetroot juice on performance.

Objective: To investigate whether an acute intake of beetroot juice (BJ) improves the performance of competitive swimmers in a repeated maximum swimming effort.

Method: Thirteen national-level swimmers (six females and seven males), participated in this randomized, double-blind crossover study. In two different trials, swimmers ingested a 70-mL placebo shot (.04 mmol NO3−; PLA) or a 70-mL Beet-It shot (6.4 mmol of NO3−beet juice [BJ]) 3 h before undergoing a 6 × 100-m front-crawl maximal effort test with 7 min rest between each 100 m.

Results: Overall, 100-m times showed no difference between the BJ and PLA groups (p = .364), although a possibly shorter time was observed for BJ in the last repetition (p = .104; mean difference [MD] = −.99 s, mean-based inference [MBI] = 49/51/0). Participants in the BJ condition showed a possibly lower rate of perceived exertion in the first (p = .242, MD = −.85, MBI = 70/28/2) and second repetitions (p = .165, MD = 1.15, MBI = 83/16/1), whereas Total Quality Recovery scale scores were likely higher in the first (p = .110, MD = 1.15, MBI = 83/16/1) and third (p = .082, MD = −.77, MBI = 70/29/1) repetitions compared with those in the PLA group. Blood lactate concentration [La+] levels showed no differences between groups in any of the repetitions (p > .05, unclear), and we observed an increase in 100-m times for both BJ and PLA (BJ: p = .014, MD = −1.51 s; PLA: p = .029, MD = −1.57 s) after the fifth repetition.

Conclusion: No clear differences in performance were observed in a 6 × 100-m repeated sprint test by competitive swimmers when supplementing (or not) with BJ. However, there was a trend toward a better recovery between efforts and a better tolerance of fatigue when swimmers ingested BJ.
Effects of different external cooling placements prior to and during exercise on athletic performance in the heat: A systematic review and meta-analysis
Authors: Jiang Dongting , Yu Qiuyu , Liu Meng , Dai Jinjin
Published: January 2023

Background: Nowadays, many high-profile international sport events are often held in warm or hot environments, hence, it is inevitable for these elite athletes to be prepared for the challenges from the heat. Owing to internal cooling may cause gastrointestinal discomfort to athletes, external cooling technique seems to be a more applicable method to deal with thermal stress. Central cooling mainly refers to head, face, neck and torso cooling, can help to reduce skin temperature and relieve thermal perception. Peripheral cooling mainly refers to four limbs cooling, can help to mitigate metabolic heat from muscular contrac to effectively prevent the accumulation of body heat. Hence, we performed a meta-analysis to assess the effectiveness of different external cooling placements on athletic performance in the heat

Methods: A literatures search was conducted using Web of Science, MEDLINE and SPORTDiscus until September 2022. The quality and risk of bias in the studies were independently assessed by two researchers.

Results: 1,430 articles were initially identified (Web of Science = 775; MEDLINE = 358; SPORTDiscus = 271; Additional records identified through other sources = 26), 60 articles (82 experiments) met the inclusion criteria and were included in the final analysis, with overall article quality being deemed moderate. Central cooling (SMD = 0.43, 95% CI 0.27 to 0.58, p < 0.001) was most effective in improving athletic performance in the heat, followed by central and peripheral cooling (SMD = 0.38, 95% CI 0.23 to 0.54, p < 0.001), AND peripheral cooling (SMD = 0.32, 95% CI 0.07 to 0.57, p = 0.013). For the cooling-promotion effects on different sports types, the ranking order in central cooling was ETE (exercise to exhaustion), TT (time-trial), EWT (exercise within the fixed time or sets), IS (intermittent sprint); the ranking order in peripheral cooling was EWT, TT, ETE and IS; the ranking order in central and peripheral cooling was ETE, IS, EWT and TT.

Conclusion: Central cooling appears to be an more effective intervention to enhance performance in hot conditions through improvements of skin temperature and thermal sensation, compared to other external cooling strategies. The enhancement effects of peripheral cooling require sufficient re-warming, otherwise it will be trivial. Although, central and peripheral cooling seems to retain advantages from central cooling, as many factors may influence the effects of peripheral cooling to offset the positive effects from central cooling, the question about whether central and peripheral cooling method is better than an isolated cooling technique is still uncertain and needs more researchs to explore it.
Tapped area detection and new tapping line location for natural rubber trees based on improved mask region convolutional neural network
Authors: Chen Yaya , Zhang Heng , Liu Junxiao , Zhang Zhifu , Zhang Xirui
Published: January 2023

Aiming at the problem that the rubber tapping robot finds it difficult to accurately detect the tapped area and locate the new tapping line for natural rubber trees due to the influence of the rubber plantation environment during the rubber tapping operation, this study proposes a method for detecting the tapped area and locating the new tapping line for natural rubber trees based on the improved mask region convolutional neural network (Mask RCNN). First, Mask RCNN was improved by fusing the attention mechanism into the ResNeXt, modifying the anchor box parameters, and adding a tiny fully connected layer branch into the mask branch to realize the detection and rough segmentation of the tapped area. Then, the fine segmentation of the existing tapping line was realized by combining edge detection and logic operation. Finally, the existing tapping line was moved down a certain distance along the center line direction of the left and right edge lines of the tapped area to obtain the new tapping line. The tapped area detection results of 560 test images showed that the detection accuracy, segmentation accuracy, detection average precision, segmentation average precision, and intersection over union values of the improved Mask RCNN were 98.23%, 99.52%, 99.6%, 99.78%, and 93.71%, respectively. Compared with other state-of-the-art approaches, the improved Mask RCNN had better detection and segmentation performance, which could better detect and segment the tapped area of natural rubber trees under different shooting conditions. The location results of 560 new tapping lines under different shooting conditions showed that the average location success rate of new tapping lines was 90% and the average location time was 0.189 s. The average values of the location errors in the x and y directions were 3 and 2.8 pixels, respectively, and the average value of the total location error was 4.5 pixels. This research not only provides a location method for the new tapping line for the rubber tapping robot but also provides theoretical support for the realization of rubber tapping mechanization and automation.
Variation in scent amount but not in composition correlates with pollinator visits within populations of deceptive Arum maculatum L. (Araceae)
Authors: Gfrerer Eva , Laina Danae , Gibernau Marc , Comes Hans Peter , Hörger Anja C. , Dötterl Stefan
Published: January 2023

Floral scent is vital for pollinator attraction and varies among and within plant species. However, little is known about how inter-individual variation in floral scent affects the abundance and composition of floral visitor assemblages within populations. Moreover, for deceptive plants it is predicted that intra-population variation in scent can be maintained by negative frequency-dependent selection, but empirical evidence is still lacking. To investigate the ecological and evolutionary relations between inter-individual scent variation (i.e., total emission and composition) and floral visitors in deceptive plants, we studied floral scent, visitor assemblages, and fruit set in two populations of fly-pollinated (Psychodidae, Sphaeroceridae; Diptera) and deceptiveArum maculatumfrom Austria (JOS) and northern Italy (DAO). By correlating individual data on floral scent and visitor assemblages, we show that inter-individual variation in floral scent partly explains variation in visitor assemblages. The quantity of floral scent emitted per individual correlated positively with visitor abundance in both populations but explained visitor composition only in DAO, where strongly scented inflorescences attracted more sphaerocerid flies. However, in each population, the composition of floral scent did not correlate with the composition of floral visitors. There was also no evidence of negative frequency-dependent selection on floral scent. Instead, in JOS, more frequent scent phenotypes attracted more pollinators and were more likely to set an infructescence than rarer ones. Our results show that floral scent, despite being key in pollinator attraction inA. maculatum, only partly explains variation in pollinator abundance and composition. Overall, this study is the first to shed light on the importance of inter-individual variation in floral scent in explaining floral visitor assemblages at the population level in a deceptive plant species.
Development of a MAGIC population and high-resolution quantitative trait mapping for nicotine content in tobacco
Authors: Yuan Guangdi , Sun Kefan , Yu Wenlong , Jiang Zipeng , Jiang Caihong , Liu Dan , Wen Liuying , Si Huan , Wu Fengyan , Meng He , Cheng Lirui , Yang Aiguo , Wang Yuanying
Published: January 2023

Multiparent Advanced Generation Inter-Cross (MAGIC) population is an ideal genetic and breeding material for quantitative trait locus (QTL) mapping and molecular breeding. In this study, a MAGIC population derived from eight tobacco parents was developed. Eight parents and 560 homozygous lines were genotyped by a 430K single-nucleotide polymorphism (SNP) chip assay and phenotyped for nicotine content under different conditions. Four QTLs associated with nicotine content were detected by genome-wide association mapping (GWAS), and one major QTL, named qNIC7-1, was mapped repeatedly under different conditions. Furthermore, by combining forward mapping, bioinformatics analysis and gene editing, we identified an ethylene response factor (ERF) transcription factor as a candidate gene underlying the major QTL qNIC7-1 for nicotine content in tobacco. A presence/absence variation (PAV) at qNIC7-1 confers changes in nicotine content. Overall, the large size of this MAGIC population, diverse genetic composition, balanced parental contributions and high levels of recombination all contribute to its value as a genetic and breeding resource. The application of the tobacco MAGIC population for QTL mapping and detecting rare allelic variation was demonstrated using nicotine content as a proof of principle.