bluuebunny/crossref_metadata_2025 huggingface dataset:
Biospecimen Donation in Biobank Construction: Aspects Affecting Donation and Publics’ Concern
Authors: Zhou Ziyu , Yu Haiyang , Zhang Yun , Qin Bo , Huang Jufang
Published: November 2019

Background A biobank is a storage facility which stores sample mainly for biological or medical researches. Biospecimen collection is the kind of work essential in the building of biobank. However, we found the collection works had the difficulties in the proceeding because of the low anticipation rate. Methods We here conducted a questionnaire including several questions covered several aspects in order to find out people’s attitude towards biospecimen donation. The questionnaire had 20 questions mainly focused on overall publics’ participation rates, matters that influence their participation and major publics’ concerns in biospecimen donation. Results In our survey, 1477 from 2200 distributed questionnaires were responded, and electronic questionnaires showed the highest response rate of 49.9%. In all respondents, 936 showed willingness in participation, providing a percentage of 63.4%. We found that most respondents lack the knowledge of biospecimen donation and biobanking but still have a positive attitude towards biospecimen donation. Several factors, including family disease history( p <0.05), previous donation history ( p <0.01), the knowledge of biospecimen donation ( p <0.01), the knowledge of biospecimen donation conception ( p <0.01) were closely linked to donation willingness. Among those factors, family disease history, brief knowledge about biospecimen donation were independent factors affecting donation willingness. The reverse health effects and privacy leakage were major concerns among the majority of respondents. Primary reasons affected willingness, or unwillingness donation were their benefic to public interests and privacy concerns. In summary, this survey we mainly discussed factors affecting publics’ willingness and issues people concerned most in biospecimen donation of a biobank. Conclusions Most respondents hold positive attitudes towards biospecimen donation but lack relevant knowledges. Several factors influenced donation willingness probably caused by those deficiency knowledge. Even though faced this challenge, responders’ merits of altruistic behavior may contribute to the act of donation. In addition, information leakage and health impairment remained the domain factors prevented their participation. Further works are required to eliminate those undesirable elements restrained biospecimen donation by the well biobank knowledge popularization and detailed pre-donation information exchange .
Pharmacological inhibition of APP processing and knock-down of APP in primary human macrophages impairs the secretion of cytokines
Authors: Spitzer Philipp , Walter Matthias , Kranig Caroline , Oberstein Timo Jan , Linning Philipp , Knölker Hans-Joachim , Kornhuber Johannes , Maler Juan Manuel
Published: November 2019

Background

The amyloid precursor protein (APP) and amyloid β (Aβ) peptides support the innate immune defense as an immune receptor, an antimicrobial peptide and an opsonine. In APP-deficient mouse models, a reduced secretion of cytokines has been observed. It is unclear whether this can be attributed to the lack of APP or to the missing secretion of Aβ peptides.Methods

In primary human monocyte derived macrophages the secretion of Aβ peptides was inhibited by the γ-secretase inhibitor DAPT or by the β-secretase inhibitor GL-189, applied as a tripartite substance. Alternatively, APP was knocked down by transfection with siRNA. TNFα, IL-6 and IL-10 were measured by ELISA and the phagocytotic activity was evaluated by flow cytometry.Results

Reduced concentrations of TNFα and IL-6 were observed in the media of APPk/d macrophages and after inhibition of the β-, or γ-secretase, especially after additional immunological activation with LPS. Secretion of IL-10 was increased after pharmacological inhibition of APP processing when the macrophages were not immunologically activated but was decreased during LPS-induced inflammation in APPk/d macrophages. No changes of the phagocytotic activity were observed.Conclusion

We conclude that macrophage APP and Aβ peptides support the initiation of an immune response and are involved in the secretion of TNFα, IL-6 and IL-10.
Long non-coding RNA DIO3OS binds to microRNA-130b to restore radiosensitivity in esophageal squamous cell carcinoma by upregulating PAX9
Authors: Liu Jun-Qi , Yang Xiang-Xiang , Guo Yue-Xin , Wang Xin , Gu Hao , Zhang Song , Gao Liang , Zhao Di , Fan Rui-Tai
Published: November 2019

Background: Esophageal squamous cell carcinoma (ESCC) ranks as one of the most fatal cancers worldwide for its aggression and unsatisfactory survival rate. The long non-coding RNA (lncRNA)-microRNA (miRNA)-mRNA axis has been highlighted as a potency biomarker for enhancing the radiosensitivity of ESCC. Hence, we investigated the functional mechanism of the DIO3OS/miR-130b/paired box 9 (PAX9) axis in the radioresistance of ESCC cells. Methods: In cell experiments, we altered the miR-130b expression in ESCC cells using mimics or inhibitors to examine its effects on ESCC cell activities in response to 4 Gy irradiation, as well as the involvement of DIO3OS and PAX9. Tumor xenograft experiments were then conducted to observe the effect of miR-130b, DIO3OS and PAX9 on radiosensitivity of ESCC ells in vivo . Results: miR-130b was found to be highly-expressed in the ESCC. Downregulated miR-130b inhibited proliferation, invasion and resistance to apoptosis in ESCC cells. DIO3OS and PAX9 were reduced in ESCC. A notable finding revealed that miR-130b could bind to DIO3OS and PAX9 respectively. DIO3OS could upregulate PAX9 by binding to miR-130b, which ultimately promoted the radiosensitivity of ESCC in vitro and in vivo . Conclusion: Taken together, DIO3OS upregulates the expression of PAX9 by binding to miR-130b, ultimately promoting the radiosensitivity of ESCC. Keywords: DIO3OS. MicroRNA-130b. Paired box 9. Radiosensitivity. Esophageal squamous cell carcinoma.
Informal caregivers’ views on the quality of health care services provided to older patients aged 80 or more in the hospital and 30 days after discharge
Authors: Lilleheie Ingvild , Debesay Jonas , Bye Asta , Bergland Astrid
Published: November 2019

Background In the European Union (EU), informal caregivers provide 60% of all care. Informal caregiving ranges from assistance with daily activities and provision of direct care to helping care recipients to navigate within complex healthcare and social services systems. While recent caregiver surveys document the impact of informal caregivers, systematic reviews show that they have unmet needs. Because of the political desire to reduce the length of hospital stays, older patients are discharged from the hospital ‘quicker and sicker’ than before. The transition between different levels of the healthcare system and the period after hospital discharge is critical for elderly patients. Caregivers’ perspectives on the quality of older patients’ care journeys between levels of the healthcare system may provide valuable information for healthcare providers and policymakers. This study aims to explore older patient’s informal caregivers’ views on healthcare quality in the hospital and in the first 30 days after hospitalisation. Method We used a phenomenological perspective to explore and describe informal caregivers’ subjective experiences of providing care to older relatives. We conducted semi-structured individual interviews with 12 participants. The interviews were then transcribed and analysed thematically. Results The analysis yielded the overarching theme ‘Relatives – a health service alliance – quality creator’, which was divided into four main themes: ‘Fast in, fast out’, ‘Scant information’, ‘Disclaimer of responsibility’ and ‘A struggle to secure professional care’. The healthcare system seemed to pay little attention to ensuring mutual understandings between those involved in discharge, treatment and coordination. The participants experienced that the healthcare providers’ main focus was on the patients’ diseases, although the health services are supposed to view patients holistically. Conclusion Based on the information given by informal caregivers, health services must take into account each person’s needs and preferences. To deliver quality healthcare, better coordination between inter-professional care teams and the persons they serve is necessary. Health professionals must strengthen the involvement of caregivers in transitions between care and healthcare. Future work should evaluate targeted strategies for formal caregivers to cooperate, support and empower family members as informal caregivers.
Longitudinal association between housing accessibility and activities of daily living: the role of self-efficacy and control in people ageing with Parkinson’s disease
Authors: Gefenaite Giedre , Björk Jonas , Iwarsson Susanne , Slaug Björn , Schmidt Steven M. , Nilsson Maria H
Published: November 2019

Objectives External housing-related control beliefs (HCB) and general self-efficacy (GSE) influence different health outcomes in the general ageing population, but there is no information of their role in people ageing with Parkinson’s disease (PD). This study aimed to longitudinally assess the role of external HCB and GSE on the association between housing accessibility and activities of daily living (ADL) among people ageing with PD.Methods Baseline and 3-year follow-up data on 130 community-living participants from the Swedish project ‘Home and Health in People Ageing with PD’ were collected. Assessments addressed housing accessibility, external HCB, GSE, generic ADL and ADL specific to PD. The moderating effects of external HCB and GSE were assessed by including an interaction term in multivariable logistic regression.Results There were statistically significant interactions between housing accessibility and GSE on ADL (p=0.03), and housing accessibility and external HCB on PD specific ADL (p=0.03). After stratifying the analyses by GSE, housing accessibility problems led to more dependence and difficulty in ADL in participants with low GSE (OR 1.14; 95% CI 0.98-1.32). After stratifying by external HCB, housing accessibility increased dependence and difficulty in PD specific ADL in participants with low external HCB (OR 1.35; 95% CI 1.03-1.76).Discussion The results suggest causal links between housing accessibility and ADL in people with PD with different moderators, namely GSE and external HCB, playing a role for generic ADL and ADL specific to PD. Further studies should validate these findings and explore their potential application in PD-related care and rehabilitation.
Comparison of the influence of pupil dilation on predicted postoperative refraction and recommended intraocular lens power among Barrett Universal II, Haigis, and SRK/T calculation formulas: a retrospective study
Authors: Teshigawara Takeshi , Meguro Akira , Mizuki Nobuhisa
Published: November 2019

Background We investigated the effect of pupil dilation on predicted postoperative refraction (PPR) and recommended intraocular lens (IOL) power calculated using three different generations of IOL power calculation formulas: Barrett Universal II (Barrett) (new generation), Haigis (4th generation), and SRK/T (3rd generation).Methods This retrospective study included 150 eyes. The following variables were measured and calculated using an optical biometer before and after dilation: anterior chamber depth (ACD), lens thickness (LT), white-to-white (WTW), mean absolute change (MAC) in PPR, and recommended IOL power. PPR and recommended IOL power were calculated by Barrett, Haigis, and SRK/T IOL calculation formulas. Correlations between all changes were analyzed. The influence of pupil dilation on recommended IOL power calculated by each formula was also analyzed.Results MAC in PPR before and after dilation was highest in Barrett, followed by Haigis and SRK/T. Significant differences were found among each MAC. Significant changes were observed before and after dilation in ACD and LT but not in WTW. In Barrett and Haigis, there was a significant positive correlation between change in PPR and change in ACD and a negative correlation between change in PPR and change in LT. Correlations were strongest in Barret followed by Haigis, especially in LT. Change in PPR in Barrett also demonstrated a significant positive correlation with change in WTW. The recommended IOL power using Barrett and Haigis changed before and after dilation in 23.3% and 19.3% cases; SRK/T showed no change.Conclusions In PPR and recommended IOL power, pupil dilation influenced Barrett most strongly, followed by Haigis and SRK/T. Given the stronger correlation between the change in PPR in Barrett and the change in ACD, LT, and WTW, the change of ACD, LT, and WTW is more important to the influence of dilation on Barrett. The influence of dilation on each formula and variables, including ACD, LT, and WTW is key to improving IOL calculation.
Effect on rabbits’ intraocular structure by cross-linked hyaluronic formations as vitreous substitute
Authors: Gong Yan , Chen Kai , Wu Yue , Guo Xiaohong , Zhang Tao
Published: November 2019

Purpose: To observe the effects of cross-linked hyaluronic hydrogel on retina struct and intraocular biocompatibility in rabbits. Methods: the polymer-derived hyaluronic acid which was formed by UV light cross-linked with N-vinyl-pyrrolidone. Free hyaluronic acid levels were detected by spectrophotometric method. Vitrectomy was then performed in the rabbits, and then cross-linked hyaluronic acid hydrogels of different concentrations were injected. Intraocular pressure measurements, cornea check-up, and B-ultrasound examination were performed during the follow-up period. After six weeks’ follow-up, the rabbits were sacrificed, and both eyes were removed. The eyeballs were fixed for HE staining, and the polymer materials were observed under electron microscopy. Results: The synthetic hydrogel present a transparent substance with a refractive index similar to that of the human vitreous body, which also had sufficient viscosity and elasticity for intraocular usage by rheological measurements. The results of the free hyaluronic acid test showed that the hydrogel had little degrade within one month. The results of the histology, intraocular pressure, B-ultrasound and retinal ultrastructure suggested that cross-linked hyaluronic acid hydrogel had superior tissue biocompatibility intraocular for six weeks. Conclusions: Hyaluronic acid-based cross-linked biopolymers had good biocompatibility in rabbit, which also shown promising potential as vitreous substitutes in clinical practice.
Comparison of amyloid PET measured in Centiloid units with neuropathological findings in Alzheimer’s Disease
Authors: Amadoru Sanka , Doré Vincent , McLean Catriona A , Hinton Fairlie , Shepherd Claire E , Halliday Glenda M , Leyton Cristian E , Yates Paul A , Hodges John R , Masters Colin L , Villemagne Victor L , Rowe Christopher C
Published: November 2019

Background: We aimed to determine the Centiloid unit (CL) thresholds for sparse and moderate density neuritic plaques.

Methods: Amyloid PET results in CL for 49 subjects were compared with post-mortem neuritic plaque density, visual read, and final clinicopathological diagnosis. A Youden Index was used to determine the optimal CL thresholds from receiver operator characteristic (ROC) curves.

Results: A threshold of 20.1 CL yielded highest accuracy in detecting moderate or frequent plaque density (ROC AUC 0.97). A threshold of 9.5 CL was optimal for detecting sparse, moderate or frequent plaques (ROC AUC 0.96). Those cases with a final clinicopathological diagnosis of Alzheimer’s disease yielded a median CL result of 87.7 (IQR ±42.2) with 94% > 45 CL. Positive visual read agreed highly with results >26 CL.

Conclusions: In this cohort, values <9.5 CL accurately reflected the absence of any neuritic plaques, and >20.1 CL indicated the presence of at least moderate plaque density. Clinicopathological diagnosis of AD was rare with CL <45.
Governmental financial investment strategy of traditional medicine hospitals: evidence from public Traditional Chinese Medicine hospitals (PTHs) of Henan Province, China
Authors: Ren Weicun , Fu Xiaoli , Kasanga Maisa , Tarimo Clifford Silver , He Zhuowen , Wang Yanqing , Miao Yudong , Wu Jian
Published: November 2019

Background: Chinese government has been continually increasing its investments on Public Traditional Chinese Medicine hospitals (PTHs) in recent years. This study aimed to assess the scales and structure of investments on PTHs in Henan Province, China, in order to analyze the contribution of Government Financial Investment (GFI) to the revenue growth of PTHs, as well as raising practical investment strategies for decision-makers. Methods: This study was a panel data research, conducted in Henan Province, China. By collecting 143 PTHs’ operational data from 2005 to 2017, the authors computed data with Barrow Economic Growth Model (BEG), Stochastic Frontier Analysis (SFA) and Vector Autoregressive Model (VAR) respectively. Results: The study observed that the contribution of GFI to PTHs’ revenue growth was positive (average MPG=2.84), which means the scale of GFI hadn’t reached an optimal level. The scales of GFI on Grade III, Grade II A, Grade II B PTHs need to be increased by -5.96%, 4.88% and 11.51% respectively in order to maximize the input-output efficiency. The third year after the investment year may be a more effective period for conducting an effect evaluation of GFI in Henan Province. Conclusions: GFI on PTHs usually has a long-term impact on PTHs. Government needs to adjust its GFI policy to maximize the input-output efficiency.
Esbeltez y fertilización mineral en plantaciones de Pinus caribaea en Cuba
Authors: León Sánchez Maria Amparo , Reyes Pozo Jorge Luis , Pérez-León Víctor Ernesto , Bonilla Vichot Marta , Herrero-Echavarría Grisel
Published: November 2019

En el presente trabajo se estudia la respuesta de la esbeltez a la fertilización mineral fraccionada en plantaciones de Pinus caribaea var caribaea Morelet y Golfari sobre suelos franco-arenosos en el occidente de Cuba. La fórmula de fertilizante utilizada fue NPK (8-10-10). Se establecieron siete tratamientos diferenciados por las dosis de fertilizante y regímenes de aplicación, alterno o continuo, más un tratamiento testigo sin fertilización. Se tomaron medidas de altura total y diámetro normal y se calculó el índice de esbeltez a los 6 años, 15 años y 35 años de edad a un total de 288 árboles a los que se dio seguimiento. A los 6 años de edad, los valores medios de esbeltez fueron significativamente superiores con la aplicación de una dosis única de 300 g árbol-1 de (NPK); a los 35 años el promedio resultó estadísticamente inferior a 80 en los tratamientos a los que se aplicaron dosis de fertilizantes de 600 g árbol-1, 800 g árbol-1 o 1000 g árbol-1 en régimen alterno, los que alcanzaron los valores más bajos de esbeltez. Se encontró una relación lineal entre esbeltez y diámetro a 1,30 m en tratamientos con fertilización y relación logarítmica en el tratamiento sin fertilización. Se evidenció una relación directa entre las proporciones de árboles con esbeltez inferior a 80 y la de árboles en pie. Los individuos con esbeltez entre 70 y 90 alcanzaron los mayores volúmenes de madera.
FORMULATION AND EVALUATION OF ORODISPERSIBLE TABLETS (ODTS) OF DICLOFENAC SODIUM BY USING SUPERDISINTEGRANT FROM NATURAL ORIGIN
Authors: PANDA SATYAJITH , HEMALATHA NODAGALA , SHANKAR PANCHAGNULA UDAYA , BARATAM SRINIVASA RAO
Published: November 2019

Objective: In this study, a polysaccharide isolated from the seeds of Cajanus cajan (pigeon pea) was investigated as a super disintegrant in the orodispersible tablets of diclofenac sodium.

Methods: Diclofenac sodium tablets were prepared separately using different concentrations (5%, 7.5%, 10%, and 15% w/w) of isolated Cajanus cajan seed polysaccharide (natural) and sodium starch glycolate (synthetic) as super disintegrant by the direct compression method. Evaluation of tablets was done for various pre-and post-compression parameters. The stability studies were performed on optimized formulation F5. The disintegration time and in vitro drug release of the formulation F5 was compared with pregelatinized starch and synthetic super disintegrant (sodium starch glycolate).

Results: The drug-excipient interactions were characterized by Fourier transform infrared studies. The Optimized formulation F5 containing 15% polysaccharide showed wetting time of 118.7 seconds with 105.3 seconds of disintegration time and 95.61% dissolved in 3 min.

Conclusion: The present work revealed that Cajanus cajan seed polysaccharide has a good disintegrating agent in the formulation of orodispersible tablets.
CHARACTERIZATION AND ANTI-NEOPLASTIC POTENTIAL OF PHYTOFABRICATED SILVER NANOPARTICLES ON HUMAN BREAST CANCER CELL LINE (MCF-7)
Authors: AMUDHA CHINNAIAH KUPPUSWAMY , DEEBA FARAH , RAJARAJAN PETHANNAN
Published: November 2019

Objective: The surging cancer incidence has necessitated the synthesis, characterization and production of green synthesized silver nanoparticles and its effective use. The present study was carried out to assess the antineoplastic potential of the green synthesized silver nanoparticles against breast cancer cell lines.

Methods: The rapid biological synthesis of silver nanoparticles was done using Withania somnifera, Aegle marmelos and Taraxacum officinale. The synthesized nanoparticles were characterized using Scanning Electron Microscope (SEM), Energy Dispersive X-Ray Spectroscopy (EDX) and Fourier Transform Infra-Red (FTIR) analysis. Further, the antineoplastic potential of the green synthesized nanoparticles were assessed by MTT assay at an ascending concentration of 25, 50, 100, 250, 500μg/ml against MCF-7 (Breast) cancer cell lines.

Results: The synthesized nanoparticles were characterized using SEM, EDX and FTIR analysis which confirmed the formation of silver nanoparticles and were of irregular shape with a size of 26 nm, 18 nm, 94 nm, and its IC50 value was 251.2563±0.59µg/ml, 215.5172±0.41µg/ml and 205.7613±0.37µg/ml for Aegle marmelos, Taraxacum officinale and Withania somnifera respectively against MCF-7 cells.

Conclusion: The investigation unveils the fact that phytofabricated AgNPs could be exploited as an effective antineoplastic agent for further medical and pharmaceutical applications.
FORMULATION AND PHYSICAL CHARACTERIZATION OF CURCUMIN NANOPARTICLE TRANSDERMAL PATCH
Authors: SURYANI  , MUSNINA WA ODE SITTI , RUSLIN  , NISA MICHRUN , APRIANTI RIMA , HASANAH MARGANITA , PUTRI FIRDA RAHMANIA , ADJENG ANDI NAFISAH TENDRI , YUNIAR NANI , SAHUMENA MUHAMAD HANDOYO , ASWAN MUHAMMAD
Published: November 2019

Objective: This study was conducted to formulate curcumin nanoparticles transdermal patches and to evaluate their physical characterization.

Methods: Curcumin nanoparticles transdermal patches were formulated by the casting evaporation method. Transdermal patches were made using combinations of hydroxypropyl methylcellulose (HPMC) and ethyl cellulose (EC) at ratios of 4.5:1.5 for Formula 1 (F1), 4:1 for Formula 2 (F2), 3.5:1.5 for Formula 3 (F3), 3:2 for Formula 4 (F4), and 2.5:2.5 for Formula 5 (F5). Physical characterization evaluation (organoleptic properties, pH, weight uniformity, thickness uniformity, percent moisture content, and tensile strength) was then performed. The permeation of curcumin nanoparticles into the skin was evaluated using Franz diffusion cells.

Results: Curcumin nanoparticles transdermal patches could be formulated by the casting evaporation method with the organoleptic properties characterized as smooth, dry, yellow in color, having menthol odor, and transparent. The pH values ranged between 5.0 and 6.0. The thickness of the patches ranged from 0.1 to 0.2 mm. The average of the patches’ weight was 0.7 g, and the percent moisture content ranged from 1.0 to 6.0%. The tensile strength values were 1.0 to 2.0 N/mm. Curcumin nanoparticles could penetrate into the skin with flux values being 1.271 µg. cm-2 (F1), 0.938 µg. cm-2 (F2), 0.775 µg. cm-2 (F3), 0.837 µg. cm-2 (F4), and 0.569 µg. cm-2 (F5).

Conclusion: All patches met the requirement of the physical characterization for the transdermal patch.
FORMULATION DEVELOPMENT OF COLON TARGETED MESALAMINE PELLETS: IN VITRO-IN VIVO RELEASE STUDY
Authors: BAHEKAR JAGAN , WADHER SHAILESH
Published: November 2019

Objective: This study was intended to investigate the potential of the colon specificity approach comprising of use of pH-sensitive and time-dependent polymers in combination for precise colonic release of Mesalamine or 5-Aminosalicylic acid (5-ASA).

Methods: The extrusion and spheronization method, preferably employed in industry for allowing high dose capacity to formulate, was used to prepare drug pellets. The Wurster coating technique used for aqueous coatings of Eudragit NE 40D as an inner coat and Eudragit FS30D as outer coat. The changing pH media used for in vitro release study of optimization batches for both the coating levels. A scanning electron microscope (SEM) was used to evaluate coating thickness and surface morphology.

Results: The pharmacokinetic parameters of formulation evaluated by in vivo study in rabbits revealed that the uncoated formulation released the drug too early in the gastrointestinal tract (GIT) with a mean Cmax of 1205.28±0.37 µg/ml at 2 h after administration, whereas desired lag time was achieved in case of coated pellets exhibiting mean Cmax 465.94±0.21 µg/ml and tmax of 8 h.

Conclusion: The in vitro and in vivo release study divulge the reliability of approach involving the use of pH sensitivity and time dependency of polymer for drug release in a single formulation for the treatment of colonic diseases. Hence, the present study provides constructive results for colon targeting of 5-ASA pellets with industrially feasible processes.
COMPARATIVE STUDY OF FATTY ACID PROFILES IN PATIN (PANGASIUS MICRONEMUS) AND GABUS (CHANNA STRIATA) FISH OIL AND ITS AUTHENTICATION USING FTIR SPECTROSCOPY COMBINED WITH CHEMOMETRICS
Authors: PUTRI ANGGITA ROSIANA , ROHMAN ABDUL , RIYANTO SUGENG
Published: November 2019

Objective: The aims of this research were to analyse the fatty acids contained in Patin (Pangasius micronemus) and Gabus (Channa striata) fish oils also its authentication using FTIR spectroscopy combined with chemometrics.

Methods: Patin fish oil (PFO) was extracted from patin flesh using the maceration method with petroleum benzene as the solvent, while gabus fish oil (GFO) was purchased from the market in Yogyakarta. The analysis of fatty acid was done using gas chromatography–flame ionization detector (GC-FID). The authentication was performed using FTIR spectrophotometer and chemometrics methods. Principal component analysis (PCA) was used to determine the proximity of oils based on the characteristic similarity. The quantification of adulterated PFO was performed using multivariate calibrations, partial least square (PLS) and principal component regression (PCR). The classification between authentic oils and those adulterated used discriminant analysis (DA).

Results: The level of saturated and polyunsaturated fatty acids in PFO is higher than in GFO. The PLS and PCR methods using the second derivative spectra at wavenumbers of 666–3050 cm-1 offered the highest values of coefficient of determination (R2) and lowest root means the square error of calibration (RMSEC) and root mean square error of prediction (RMSEP).

Conclusion: The PCA method was successfully used to determine the proximity of oils. Among oils studied, PFO has a similarity fatty acid composition with GFO. The DA method was able to screen pure PFO from adulterated PFO without any misclassification reported. FTIR spectroscopy in combined with chemometrics can be used for authentication and quantification.
FORMULATION AND CHARACTERIZATION OF TIMOLOL MALEATE-LOADED NANOPARTICLES GEL BY IONIC GELATION METHOD USING CHITOSAN AND SODIUM ALGINATE
Authors: AHDYANI RISA , NOVITASARI LARAS , MARTIEN RONNY
Published: November 2019

Objective: The objectives of this study were to formulate and characterize nanoparticles gel of timolol maleate (TM) by ionic gelation method using chitosan (CS) and sodium alginate (SA).

Methods: Optimization was carried out by factorial design using Design Expert®10.0.1 software to obtain the concentration of CS, SA, and calcium chloride (CaCl2) to produce the optimum formula of TM nanoparticles. The optimum formula was characterized for particle size, polydispersity index, entrapment efficiency, Zeta potential, and molecular structure. Hydroxy Propyl Methyl Cellulose (HPMC) K15 was incorporated into optimum formula to form nanoparticles gel of TM and carried out in vivo release study using the Franz Diffusion Cell.

Results: TM nanoparticles was successfully prepared with concentration of CS, SA, and CaCl2 of 0.01 % (w/v), 0.1 % (w/v), and 0.25 % (w/v), respectively. The particle size, polydispersity index, entrapment efficiency, and Zeta potential were found to be 200.47±4.20 nm, 0.27±0.0154, 35.23±4.55 %, and-5.68±1.80 mV, respectively. The result of FTIR spectra indicated TM-loaded in the nanoparticles system. In vitro release profile of TM-loaded nanoparticles gel showed controlled release and the Korsmeyer-Peppas model was found to be the best fit for drug release kinetics.

Conclusion: TM-loaded CS/SA nanoparticles gel was successfully prepared and could be considered as a promising candidate for controlled TM delivery of infantile hemangioma treatment.
FORMULATION AND EVALUATION OF GLICLAZIDE NANOSPONGES
Authors: SOLUNKE RAHUL S. , BORGE UDAY R. , MURTHY KRISHNA , DESHMUKH MADHURI T. , SHETE RAJKUMAR V.
Published: November 2019

Objective: The objective of the present study was to develop and characterize an optimal stable nanosponges of Gliclazide (GLZ) by using the emulsion solvent diffusion method and aimed to increase its bioavailability and release the drug in sustained and controlled manner.

Methods: The GLZ nanosponge was prepared by emulsion solvent diffusion method using different drug-polymer ratios (1:1 to 1:5) Eudragit S100 is used as a polymer. Differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR) estimated the compatibility of GLZ with polymer. All formulations evaluated for production yield, entrapment efficiency, in vitro drug release, scanning electron microscopy (SEM) and stability studies.

Results: The DSC and FTIR Studies revealed that no interaction between drug and polymer. The Production yield of all batches in the range of 73.8±0.30 to 85.6±0.32. Batch F3 showed the highest production yield, the entrapment efficiency of batch F3 70.6±0.77. The average particle size ranges from 303±2.36 to 680±2.50 nm. By the end of 10th hour F3 formulation shown highest drug release was found to be 94.40±1.12%. The release kinetics of the optimized formulation shows zero-order drug release. The stability study indicates no significant change in the in vitro dissolution profile of optimized formulation.

Conclusion: The results of various evaluation parameters, revealed that GLZ nanosponges would be possible alternative delivery systems to conventional formulation to improve its bioavailability, the emulsion solvent diffusion method is best method for preparation of nanosponges and release the drug in sustained and controlled manner.
OPTIMIZATION OF PCL-PEG-PCL TRIBLOCK COPOLYMER MICELLES AS HYDROPHOBIC DRUG CARRIER WITH A 22 FULL FACTORIAL DESIGN
Authors: PATMAYUNI DEWI , SULAIMAN T. N. SAIFULLAH , ZULKARNAIN ABDUL KARIM
Published: November 2019

Objective: This study aims to optimize PCL-PEG-PCL (PCEC) triblock copolymer micelles as a hydrophobic drug carrier, simvastatin (SV).

Methods: PCEC triblock copolymer was prepared by the ring-opening polymerization method (ROP) with different ɛCL/PEG ratio (2 and 5). SV was incorporated into the PCEC triblock copolymer micelles with a concentration of 2.5 and 10 % w/w by the solvent evaporation method (film formation). The influence of the ɛCL/PEG ratio and concentration of SV effect on the responses particle size (PS), polydispersity index (PI) and entrapment efficiency (EE) was assessed using 22 full factorial design method. The test results were analyzed using Design-Expert software to obtain the optimum formula.

Result: The selection of the optimum formula is based on the desirability value, the formula with the largest desirability value is chosen as the optimum formula. The results showed the optimum formula chosen had a desirability value of 0.860 consisting of a ɛCL/PEG ratio of 5 and SV concentration of 10 % w/w, with the PS, PI dan EE value was 322.1±3.51 nm, 0.471±0.09 and 87.08±1.17 %, respectively.

Conclusion: The 22 full factorial design has been proven to be used as an optimization method to determine the optimum formula of SV-loaded PCEC triblock copolymer micelles with a good result of the PS, PI and EE responses.
DESIGN AND CHARACTERIZATION OF CANDESARTAN CILEXETIL ORAL NANOEMULSION CONTAINING GARLIC OIL
Authors: AL-SHAIBANIA ABULFADHEL JABER NEAMAH , AL-GBURIB KARRAR MOHAMMED HASAN , ALBO HAMRAHA KARRAR TALIB KHUDHAIR , ABD ALRIDHAB ALI MOHAMMED
Published: November 2019

Objective: This study was designed to prepare and characterize oil in water (o/w) nanoemulsion of candesartan cilexetil for oral administration. Preparation of candesartan cilexetil as nanoemulsion could increase its water solubility and thus could enhance its bioavailability.

Methods: Aqueous titration method was used to construct the pseudo-ternary phase diagrams of nanoemulsion (NE) consisting of oil, various weight ratios of surfactant and co-surfactant (S mix), and deionized water. Different characterization techniques were conducted on the prepared nanoemulsions to obtain the optimized formula.

Results: Characterizations of formula NE-4 (consists of 0.16% of candesartan cilexetil, 10% of garlic oil, 35 % of S mix (3:1) and 54.84% of deionized water) revealed the following characteristics: droplet size range (95-139 nm), polydispersity index (0.14), zeta potential value (-41.06 mV) and pH value (6.71), which are suitable for oral administration. Candesartan cilexetil in vitro release from this formula was significantly high (P<0.05) and scanning probe microscopy (SPM) study confirmed that the optimized formula (NE-4) was in nano-scale.

Conclusion: Nanoemulsion formula 4 (NE-4) of candesartan cilexetil is the optimized formula and it could be a promising formula for improving the water solubility of candesartan cilaxetil.
APPLICATION OF NOVEL NATURAL MUCOADHESIVE POLYMER IN THE DEVELOPMENT OF PENTOXIFYLLINE MUCOADHESIVE TABLETS
Authors: JOHNSELVARAJ GNANASEKARAN , BALASUBRAMANIAN ARUL , RAMALINGAM KOTHAI
Published: November 2019

Objective: The present study was planned to develop a mucoadhesive tablet formulation of the drug pentoxifylline using natural mucoadhesive polymer from the plant Ocimum basilicum Linn.

Methods: The isolated polymer was used to formulate the mucoadhesive tablets with 3 different concentrations. The tablets were formulated by using direct compression technique and evaluated for various parameters such as thickness, friability, weight variation, hardness, mucoadhesive strength, swelling index by standard methods, and the in vitro drug release studies in USP dissolution test apparatus type-II.

Results: The swelling index was indirectly proportional to the polymer concentration and the tablets with a high concentration of polymer showed better mucoadhesive strength (28.5532±0.4660). The in vitro drug release showed that the drug release was indirectly proportional to the polymer concentration. The formulation F3 showed the controlled release of drug pentoxifylline (99.84±1.86) for 10 h. The mechanism of drug release was found to be Fickian diffusion and followed the zero-order kinetics, which was proved by its highest linearity (r2) in all the formulations.

Conclusion: The tablets formulated with the isolated polymer of Ocimum basilicum Linn showed the good mucoadhesive mucoadhesive property and it controlled the release of the pentoxifylline.
MOLECULARLY IMPRINTED POLYMER NANOPARTICLES (MIP-NPs) APPLICATIONS IN ELECTROCHEMICAL SENSORS
Authors: FAUZI DIANE , SAPUTRI FEBRINA AMELIA
Published: November 2019

Molecularly Imprinted Polymers (MIPs) is a polymer that binds together to form a specific binding site that is selective for certain analytes. Its high stability, its synthesize simplicity, and it can ease costs significantly make it was applied widely as a receptor instead of antibodies or enzymes. MIPs can be re-developed into MIPs nanoparticles (MIP-NPs) which have greater potential. MIPs use in electrochemical sensors have relevant applications in daily life and have been tested in human samples. Electrochemical sensors have been successfully functioned with MIP-NPs leading to real-time monitoring of drugs, pesticides, environmental contaminants, and secondary metabolites, as well as molecules with biological relevance. The aim of this review is to summarize the developments and applications of MIP-NPs as a selective recognition component in electrochemical sensors with special emphasis on their analytical applications.
EFFECT PROCESSING VARIABLES ON THE CHARACTERISTICS OF ITRACONAZOLE HOLLOW MICROSPHERES
Authors: ROHILLA SURBHI , BHATT D. C. , AHALWAT SHAVETA
Published: November 2019

Objective: The purpose of the study was to develop the multiple unit non-effervescent gastroretentive floating hollow microspheres to enhance the bioavailability of the drug by varying the concentration of low-density polymer and release modifier to retaining the formulation at its absorption site. Design of experiment approach applied to get the best possible formulation with minimum assets and experimentation.

Methods: The hollow microspheres were prepared by emulsion solvent diffusion-evaporation technique using ethylcellulose as low-density polymer and Eudragit E100 as release modifier. The central composite design was used for the optimization of independent variables and was evaluated for particle size, entrapment efficiency, in vitro floating ability and drug release characteristics.

Results: The physicochemical analysis was done to confirm any interaction between drug and excipients. The Scanning Electron Microscopy (SEM) showed a smooth, spherical surface with an inner hollow cavity. The stability study proves that the hollow microspheres were more stable under different storage conditions with no significant changes in formulation. The drug release mechanism of the optimized batch can be explained by Korsmeyer Peppas model.

Conclusion: Based on the results, the hollow microspheres with a release modifying polymer offers a superior approach to retain the formulation in the stomach.
DEVELOPMENT AND CHARACTERIZATION OF CONTROLLED RELEASE TABLETS OF CANDESARTAN CILEXETIL/Β-CYCLODEXTRIN INCLUSION COMPLEX
Authors: SALIH OMAR SAEB , HAMODDI ZAHRAA M. , TAHER SALAM S.
Published: November 2019

Objective: Matrix tablet approach is one of the delivery systems intended for poorly water-soluble drugs like candesartan cilexetil. Candesartan cilexetil is a class II drug used for the treatment of hypertension.

Methods: Matrix tablets from (F1x to F18z) were prepared in the presence of β-cyclodextrin. Matrix tablet formulation ensures control release of the drug and higher dissolution by β-cyclodextrin. Fourier transform infrared spectroscopy and differential scanning calorimetry were used to study compatibility.

Results:The angle of repose determination showed good flow for most of the formulas besides having good compressibility. Weight variation test for all formulas showed accepted value. Drug content measurement showed accepted values. Friability and hardness of tablets were within the allowed values. Higher tablet swelling was obtained for the formulas containing hydroxy propyl methyl cellulose (HPMC) K100M (F3x and F15z) in which the ratio of the polymer was (1:1) and (1:3) respectively. In vitro release showed that F1x to F13z were studied depends on the type and amount of polymer i.e. (1:1), (1:2) and (1:3) respectively. F1x release after 8h was 95% which contain (1:1) polymer ratio in compare to F3x, which showed 85% after 8h, Which include 1:3 (drug: HPMC K100). Kinetic studies showed a zero-order model.

Conclusion: The use of β-cyclodextrin modify the release profile of the drug, and control the sustained release formulas. The lower the time of the release but in a range that a sustained release of the drug was observed in compare with the formulas prepared without β-cyclodextrin.
RECENT TRENDS IN MANAGEMENT OF KERATOCONJUNCTIVITIS SICCA (DRY EYE DISEASE)
Authors: SHEKAR NITHISH , GOWDA D. V. , KUMAR HITESH , JAIN GAURAV K. , JAIN VIKAS
Published: November 2019

At the air-water interface, the tear film lipid layer (TFLL), a combination of lipids and proteins plays an important role in surface tension of the tear and is necessary for the physiological hydration of the ocular surface and maintenance of ocular homeostasis. Alteration in lacrimal fluid rheology, differences in lipid constitution or down regulation of particular tear proteins are found in maximum types of ocular surface disease including dry eye disease (DED). Dry eye is a disorder of the tear film due to tear deficiency or excessive tear evaporation, which causes damage to the interpalpebral ocular surface and is associated with symptoms of discomfort. It results in changes on the ocular surface epithelia causing reduced tear quantity and surface sensitivity which leads to inflammation reactions. Managing this inflammation is very helpful in dry eye disease patients. In this article we revise the current understanding of tear film properties, ocular surface and review the effectiveness of topically applied tear supplements, thermo sensitive atelocollagen punctal plug, subtrasal ultrasonic transducers, novel liposome based gelling tear formation and insulin based ophthalmic delivery systems which help in restoring the healthy tear film.
IN VITRO ANTIBACTERIAL ACTIVITIES OF MARINE SPONGE-ASSOCIATED BACTERIA AGAINST PATHOGENIC VIBRIO SPP. CAUSES VIBRIOSIS IN SHRIMPS
Authors: WAHYUDI ARIS TRI , PRIYANTO JEPRI AGUNG , WULANDARI DIAN RETNO , ASTUTI RIKA INDRI
Published: November 2019

Objective: This study was aimed to isolate and screen marine sponge-associated bacteria producing anti-Vibrio compounds and to identify their compounds from the bacterial extract.

Methods: Sponge-associated bacteria were isolated by spread plate method. Their anti-Vibrio activity against Vibrio parahaemolyticus, V. harveyi, and V. vulnificus was determined by dual culture test. Three potential isolates were identified based on 16S-rRNA gene analysis. All isolates producing anti-Vibrio compounds was tested for their haemolytic characters in blood agar medium. Anti-Vibrio activity of the most potential isolate was also tested by using its supernatant, extract, and concentrated culture. Chemical composition of crude extract derived from that isolate was identified by GC-MS analysis.

Results: 68 bacterial isolates have been isolated from the marine sponge, Spongia sp., Svenzea sp., Ircinia sp., and Igernella sp. Of 68 isolates, 15 (22%) isolates had anti-Vibrio activities in various spectra against three Vibrio species, including V. harveyi, V. parahaemolyticus, and V. vulnificus. All isolates producing anti-Vibrio compounds were non-haemolytic. Bacterial isolates coded as D6.6, D6.19, and P4.17 have broad spectra. They could inhibit at least two Vibrio species as indicated by the clear zone formed around bacterial colonies. Based on 16S-rRNA, these isolates were closely related (similarity ≥ 99%) to Brevibacterium casei strain M Sw oHS, Bacillus altitudinis strain FJAT 47750, and Bacillus altitudinis strain PgBe190, respectively. D6.6 isolate was the most potential isolate, which could inhibit three Vibrio species. Consistently, its anti-Vibrio activity also confirmed by their supernatant, concentrated culture, and crude extract of that isolate. The crude extract derived from this isolate contained 10 major compounds that are biologically active.

Conclusion: This study suggests that 15 bacteria strains isolated from marine sponges were potentially could inhibit Vibrio’s growth in vitro. These isolate could be further explored as anti-Vibrio agent.
ASSESSING THE IMPACT ON PEFR AMONG SMOKERS AND PASSIVE SMOKERS–A COMPARATIVE STUDY
Authors: B. GANESH PANDIAN , P. SIREESHA , NG. YEN PING , G. SUSMITHA , T. SAM AASEER
Published: November 2019

Objective: The purpose of this study was to monitor the intensity and difference in Peak Expiratory Flow Rate (PEFR) between smokers and passive smokers.

Methods: A total of 1000 participants were enrolled in two groups as smokers and passive smokers who are living closely with smokers. Their PEFR values were measured with Wright’s mini peak flow meter. The influence of smoking on the lung function among smokers and passive smokers were assessed with a suitable statistical test.

Results: Among the study participants, most of the smokers were in the age group of 31 to 60 and 31 to 50 in passive smokers. Based on the lung function smokers (31%) and passive smokers (19.2%) were in the red zone, PEFR was decreased in both smokers as well as passive smokers, and the magnitude of decline was higher in passive smoking elderly individuals. The impact of passive smoking was significantly observed in all the categories of smoking history they are living with.

Conclusion: Smokers and passive smokers have equally deleterious effects on PEFR. Where passive smoking emerged as the main variable to influence airway obstruction in smokers that caused a greater reduction in PEFR.
DEVELOPMENT AND VALIDATION OF ANALYTICAL SPECTROPHOTOMETRIC AND RP-HPLC METHODS FOR THE SIMULTANEOUS ESTIMATION OF HYDROQUINONE, HYDROCORTISONE AND TRETINOIN TERNARY MIXTURE IN TOPICAL FORMULATION
Authors: JAMAL MARWA K. AL , GAZY AZZA A.
Published: November 2019

Objective: Development and validation of spectrophotometric and RP-HPLC methods for the simultaneous determination of Hydroquinone (HQ), Hydrocortisone (HC) and Tretinoin (TRT) ternary combination in pharmaceutical preparation.

Methods: The proposed spectrophotometric method was able to determine TRT directly from its absorption spectrum at 362 nm, however, HQ and HC from their first derivative spectra at 284 nm and 252 nm, respectively, without any separation step. The RP-HPLC method was developed using a C18 Sunfire© waters column with a mobile phase composed of acetonitrile: phosphate buffer (adjusted to pH 6.1 using ortho-phosphoric acid) in the ratio of 30:70 %, v/v, respectively at a flow rate of 0.8 ml/min. Quantification was based on measuring peak areas at 260 nm.

Results: The spectrophotometric method was able to selectively quantify each of HQ, HC and TRT in the ranges of 10-50 µg/ml, 2-10 µg/ml and 0.5-5 µg/ml, respectively. The RP-HPLC method was able to produce well-resolved peaks after 3.0, 8.2 and 20.2 min, in the ranges of 2-10 µg/ml, 0.1-1 µg/ml and 0.05-2 µg/ml, for HQ, HC and TRT, respectively. The obtained A, D1 or peak areas values plotted against the concentration of each of the three components showed linear response in the stated ranges. Both methods were validated in terms of linearity, LOD, LOQ, precision, accuracy and selectivity.

Conclusion: Both developed proposed methods were applied for the determination of the active ingredients in the pharmaceutical formulation and the common excipients did not interfere in the analysis. The RP-HPLC method proved to be more sensitive when compared to the applied spectrophotometric method. However, the applied spectrophotometric methods, considered as green analytical chemistry, is a simple, time-saving method that requires minimal use of a hazardous solvent.