bluuebunny/crossref_metadata_2025 huggingface dataset:
Vitamin D for Autism Spectrum Disorders:a Protocol for Systematic Review and Meta-analysis
Authors: wang Jing , Chu Xin , Hao Haoyu , Xuemei He , Chen Yu , Wang Min , Zou Zhuo , Yin Shuyue , Qin Dongdong , Liu Yun
Published: September 2021

IntroductionAutism spectrum disorder(ASD) is a neurodevelopmental disorder characterized by repetitive and stereotypic behavior, impaired social interactions and communications. An increasing number of reseachers suggested that vitamin D may be participated in the pathogenesis of ASD, and vitamin D deficiency may be one of the causes of ASD.Meanwhile, some studies have shown that vitamin D can improve the core symptoms in ASD children.On top of updating the latest systematic review on vitamin D supplementation and ASD, we aim to conduct the meta-analyses of trials on vitamin D3 supplementation and ASD. Besides, we will conduct for the first time subgroup analyses based on individual patient data collected from randomised controlled trials.Methods and analysisA systematic review and individual patient data meta-analysis on randomised placebo-controlled trials with a vitamin D3 intervention. All studies are searched from inception without time restriction. The addressed outcomes are 25-hydroxyvitamin D, Childhood Autism Rating Scaleas(CARS), Autism Behavior Checklist (ABC), Social Responsiveness Scale(SRS) and M-CHAT. Trial results will be reanalysed using Cox proportional hazard regression models and meta-analyses are performed. Cochran’s Q-Test and the I2 index will be used to statistically assess the level of heterogeneity, while sensitivity and subgroup analyses serve to identify potential causes of heterogeneity. Subgroup analyses will be conducted for age, sex, 25-hydroxyvitamin D level,vitamin D3 dosing, follow-up time,and the core symptoms of ASD. Publication bias will be assessed by funnel plots and Egger’s test. Ethics and disseminationEthical approval is not required since no human beings are involved in this systematic review. Results will be published in a peer-reviewed journal with open access. Strengths and limitations of this study1.Meta-analysis on vitamin D and ASD,we not only study the relationship between vitamin D deficiency and the occurrence of ASD, the preventive and therapeutic effects of vitamin D on ASD, but also the influence factors of vitamin D supplementation on the therapeutic effect of ASD. 2.Assess study quality, sources of heterogeneity and bias minimise the risk of bias and will gather reproducible results.3.Eligible data for meta-analysis may be limited.PROSPERO registration number:CRD42021264097
Non-Destructive Mercury Exposure Assessment in the Brandt's Hedgehog (Paraechinus Hypomelas): Spines As Indicators of Endogenous Concentrations
Authors: behrooz Reza Dahmardeh , Poma Giulia , Barghi Mandana
Published: September 2021

Due to its persistence, bioaccumulation characteristics and toxicity, environmental contamination with mercury (Hg) is of high concern for human health, living organisms and ecosystems, and its biological monitoring is highly relevant. In this study, the levels of total Hg were measured in organs, tissues and spines of 50 individuals of Brandt's hedgehog collected in Iran in 2019. The Hg median levels in kidneys, liver, muscle, and spines were 156, 47, 47 and 20 ng/g dry weight, respectively. The results showed a significant positive correlation between the levels of Hg in kidneys and liver (r = 0.519; p < 0.01) and in spines and muscle (r = 0.337, p < 0.01) and kidneys (r = 0.309, p < 0.05). Significant differences (p < 0.05) in Hg levels in organs and tissues were also observed depending on the sex, weight, length, and age of the individuals. In addition, the median levels of total Hg in kidneys of Brandt's hedgehogs from an agricultural ecotype were significantly higher (p < 0.05) than those collected from a forest ecotype, suggesting that the habitat could have a significant impact on animal contamination.
CXCL12/CXCR7/β-arrestin1 Biased Signal Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer by Repressing Mirnas Through YAP1 Nuclear Translocation
Authors: Si Mahan , Song Yujia , Wang Xiaohui , Wang Dong , Liu Xiaohui , Qu Xianjun , Yu Xinfeng
Published: September 2021

Background: CXCR7 is an atypical chemokine receptor that transmits biased signal independent of G-protein activation. However, whether CXCL12/CXCR7 biased signal activation plays an essential role in colorectal cancer (CRC) progression and metastasis remains obscure. Methods: The functional role of CXCL12/CXCR7 biased signal in CRC was investigated by RNA-sequencing, Transwell assay and in vivo tumor xenografts. YAP1 nuclear translocation and molecular mechanisms were determined by cell transfection, luciferase activity assay, immunofluorescence, coimmunoprecipitation and immunohistochemistry and RT-qPCR analysis.Results: In this study, CXCR7 CXCL12/overexpression promotes Epithelial-to-mesenchymal transition (EMT) and upregulates the expression of stem marker doublecortin-like kinase 1 (DCLK1) in CRC cells with concurrent repression of miR-124-3p and miR-188-5p. Further luciferase assay prove that these miRNAs could regulate EMT by direct targeting vimentin and DCLK1. More importantly, CXCL12/CXCR7/β-arrestin1-mediated biased signal induces YAP1 nuclear translocation, which functions as a transcriptional repressor by interacting with Yin Yang 1 (YY1) and recruiting YY1 to the promoter of miR-124-3p and miR-188-5p. Pharmacological inhibitor of YAP1 recapitulates the anti-tumorigenesis and anti-metastasis effects of YAP1 depletion upon CXCR7 activation in tumor xenografts. Clinically, the expression of CXCR7 was positively correlated with nuclear YAP1 levels and EMT markers. Conclusions: Our findings revealed the novel role of YAP1 nuclear translocation in promoting EMT of CRC by repressing miR-124-3p and miR-188-5p through CXCL12/CXCR7/β-arrestin1 biased signal activation. These findings highlight the potential of targeting YAP1 nuclear translocation in hampering CXCL12/CXCR7 biased signal-induced metastasis of CRC.
Transcriptional Regulation of the Colorectal Cancer Stem Cell Markers, Nanog and Oct4, Induced by a Thermodynamic-Based Therapy Approach
Authors: Ghorbani Zakieh , Heidari Mansour , Jafarinia Mojtaba , Rohani Mahdi , Akbari Abolfazl
Published: September 2021

Background: Cancer stem cells (CSC) play a crucial role in tumorigenesis, recurrence, metastasis, and chemoresistanc. Some studies suggest that hyperthermia and photodynamic therapy (PDT) may be effective for cancer treatment, particularly when combined with other therapeutic approaches. However, the results are conflicting. Our aim was to evaluate the effect of hyperthermia combined with PDT on colorectal CSC viability and the gene expression of the CSC markers.Results: Cell viability decrased by PDT (P=0.015) and the combination therapy (P=0.006) but not hyperthermia alone (P=0.4) compared to control. Gene expression of CSC markers significantly decreased in all therapies. Conclusion: Hyperthermia combined with PDT was more efficient in eliminating tumors than hyperthermia or PDT alone.
Satisfaction in Population-Based Cancer Screening in a Chinese Rural High-Risk Population: The Yangzhong Early Diagnosis and Treatment of Upper Gastrointestinal Cancer
Authors: Feng Xiang , Zhu Jinhua , Hua Zhaolai , Zhou Qin , Shi Aiwu , Song Tongqiu , Yao Shenghua , Qian Dongfu , Chen Ru , Wang GuiQi , Wei Wenqiang , Zhou Jinyi
Published: September 2021

Background

Screening for upper gastrointestinal cancer (UGC) effectively reduces morbidity and mortality in gastric and oesophageal cancers. It is considered one of the effective measures for cancer control in China, but studies on its functional quality are lacking. Our study assessed the quality of screening service funded by Upper Gastrointestinal Cancer Early diagnosis and treatment (UGCEDAT) and its correlation in Yangzhong People’s hospital, China.
Methods

A cross-sectional study was conducted among 516 screening users at a screening centre in Yanghzong People’s hospital from April to July 2021. The service quality questionnaire (SERVQUAL) base on the service quality gap (SQG) model was adopted. We calculated the mean scores of perceptions and expectations and their gap. To determine the association between overall SQG and related features of participants, we used a multivariate logistic regression.
Results

The average scores of screening service users’ perceptions and expectations were 4.05 and 4.55, respectively. The SQG of five dimensions (tangibles, reliability, responsiveness, assurance and empathy) were negative, and the overall SQG was − 0.51. The responsiveness dimension had the largest gap, and tangibles had the smallest gap. Occupation status (AOR:0.575; CI:0.371–0.890), health self-assessment (AOR:4.965; CI:1.352–18.232), endoscopy experience (AOR:0.551; CI:0.377–0.806), distance from screening hospital (AOR:1.847; CI:1.249–2.733) and frequency of visit (AOR:1.646; CI:1.100-2.462) were associated with the overall SQG.
Conclusions

We observed a negative gap between perceptions and expectations of the function quality of screening service, implying a high dissatisfaction across different dimensions. Service providers should take adequate measures to bridge the dimension with the largest quality gap. Meanwhile, attention should be paid to identifying the influencing factors of the overall gap and the characteristics of dimensional expectations and perceived scores to improve the effectiveness of the intervention.
Healthcare Use in Patients With Cardiovascular Disease and Depressive Symptoms – The Impact of Internet-Delivered Cognitive Behavioural Therapy. A Secondary Analysis of a RCT
Authors: Mourad Ghassan , Lundgren Johan , Andersson Gerhard , Klompstra Mats Westas , Johansson Peter
Published: September 2021

Background: Depressive symptoms in patients with cardiovascular disease (CVD) can lead to increased healthcare use and cause an economical burden for society. Thus, interventions aiming to decrease depressive symptoms in these patients could reduce the use of healthcare services. In a previous study we reported that a 9-week internet-delivered cognitive behavioural therapy (iCBT) program (n=72) compared to an online discussion forum (ODF) (n=72) had moderate to large effect on depression in CVD outpatients. However, the associations between healthcare use and depressive symptoms were not reported in that study. The aim of this study was to describe and compare the effect of iCBT compared to ODF regarding healthcare use and to identify factors impacting healthcare use in these groups.Methods: Secondary analysis of a RCT. Data on healthcare use (including outpatient clinic/primary care contacts and hospital admissions) were retrieved from care data registries in five hospitals in Southeastern Sweden. Patients were predominantly males in both groups with a mean age of 61±13 and 64±12 years in iCBT and ODF respectively.Results: The year before the intervention, the iCBT group had a mean of 31 outpatient contacts per patient compared with 21 contacts the year after. The corresponding numbers for the ODF group were 37 and 25. The decrease was 32% in both groups and did not differ significantly between the groups.Regarding hospital admissions, the iCBT group had 0.8 admissions per patient the year before and 0.6 the year after the intervention, i.e. a decrease by 25%, whereas the ODF group had 1.1 and 0.6 admissions respectively, i.e. a decrease by 45%. The difference was not significant between the groups.Improvement in depressive symptoms post intervention was significantly associated with a decrease in number of outpatient contacts in the iCBT group. In the ODF group, better mental health-related quality of life post intervention was significantly associated with a decrease in number of hospital admissions.Conclusions: Reduced depressive symptom scores following intervention were associated with lower outpatient service use, but iCBT was not superior compared to ODF in decreasing healthcare use.Trial registration: The study was registered at ClinicalTrials.gov, NCT02778074.
Artificial Neural Network Model Using Immune-infiltration Modules for Endometrial Receptivity Assessment of Implantation Failure
Authors: Li Bohan , Duan Hua , Wang Sha , Wu Jiajing , Li Yazhu
Published: September 2021

ObjectivesThis study was anchored on the state of local immune-infiltration in the endometrium, which acts as critical factors affecting embryonic implantation, and aimed at establishing novel approaches to assess endometrial receptivity for patients with IVF failure.MethodsImmune-infiltration levels in the GSE58144 dataset (n=115) from GEO were analyzed by digital deconvolution and validated by immunofluorescence (n=30), illustrating that dysregulation of the ratio of Mf1 to Mf2 is an important factor contributing to implantation failure. Then, modules most associated with M1/M2 macrophages (Mfs) and their hub genes were then selected by weighted gene co-expression network and univariate analyses, then validated by GSE5099 macrophage dataset, qPCR analysis (n=16), and western blot. It revealed that closely related gene modules dominated three biological processes in macrophages: antigen presentation, interleukin−1−mediated signalling pathway, and phagosome acidification, respectively. Their hub genes were significantly altered in patients and related with ribosomal, lysosome, and proteasomal pathways. Finally, the artificial neural network (ANN) and nomogram models were established from hub genes, of which efficacy was compared and validated in the GSE165004 dataset (n=72). Models established by the selected hub genes exhibited excellent predictive values in both datasets, and ANN performed best with an accuracy of 98.3% and an AUC of 0.975 (95% CI 0.945-1). ConclusionsMacrophages, proven to be essential for endometrial receptivity, were regulated by gene modules dominating antigen presentation, interleukin−1−mediated signalling pathway, and phagosome acidification. Selected hub genes can effectively assess endometrial dysfunction receptivity for IVF outcomes by the ANN approach.
Evaluation of Physical Properties of Mycelium-Based Bio Composites for Use As Facade Insulation Material
Authors: Dehn Frank , Kotan Engin
Published: September 2021

Background: To evaluate the usability of mycelium-based materials for construction, first tests were carried out to determine their suitability for thermal insulation. Different substrate compositions were tested for various physical properties. The compositions and experimental setups used are described in the “Methods” section. Results: Materials based on fungal mycelium were found to have promising properties for use in the construction sector. Their thermal conductivities are comparable to those of commercially available ecological insulation materials. As material properties turned out to be dependent on the substrate used for the production of mycelium materials, further optimisation is required. Conclusion: For first preliminary tests [1] to study the performance of mycelium-based materials, different additives (beech wood, rice husks, coffee silver skin, perlite rock) were added to a base substrate to determine their influence on physical properties. Compared to the characteristics of conventional thermal insulation materials, the test results obtained are rather promising and confirm suitability of mycelium-based materials for building. However, further systematic studies are needed to investigate options to improve major properties and to ensure reproducibility of mycelium materials with largely homogeneous properties.
Engaging MSM Communities in HIV Self-Testing Research and Programming in Kenya: Collaborations, Tacit Knowledge, and a Community-Based Programme Science Approach
Authors: Kombo Bernadette Kina , Thomann Matthew , Lazarus Lisa , Musyoki Helgar , Olango Kennedy , Kuria Samuel , Kyana Martin , Otieno Memory , Njiriani Margaret , Musimbi Janet , Bhattacharjee Parinita , Lorway Robert
Published: September 2021

Background: Understanding the basic principles for achieving research outcomes that are relevant to local communities is invaluable. The process allows for building of collaborative spaces and redistribution of power in knowledge production practices. This is because members of marginalized and vulnerable communities have long been critical of a research culture that has historically failed to advance meaningful collaboration and overlooks local forms of knowledge at various phases of the research processes. There has however not been a clear framework that outlines how communities should be meaningfully engaged. Rather, substantial variations in the nature of community engagements exist across research projects, thus requiring a more nuanced approach of working with communities. Methods: In this methodological paper, we describe how a community-based program science approach guided an interdisciplinary research project to inform the rollout of HIV self-testing (HIVST) among men who have sex with men (MSM) in three urban counties in Kenya. Community members and their research and programmatic partners collaborated through all phases of the research process including research design, data collection, and translation of research. Importantly, community researchers and policy makers also played an integral role in data analysis, going well beyond the conventional role of “community engagement” in global health research. Results: The study created a platform that enabled meaningful collaborations across the diverse stakeholders and allowed the MSM communities to contribute to the decisions and solutions impacting on their community. Our community-based program science approach recognizes that community researchers possess tacit knowledge, a form of expertise that formally trained researchers do not have. Conclusion: We argue that in order to fully engage with such expertise, marginalized communities must be meaningfully involved in all levels of evidence building and decision making around programs impacting their communities. We equally emphasize the importance of setting up operating norms and clear definitions of partnership roles at the initial stages of establishing collaborations. Such a move gives the community a chance to trust their capabilities and contribute more meaningfully throughout the research processes.
Gender Difference in the Incidence of Malaria Diagnosed at Public Health Facilities in Uganda
Authors: Okiring Jaffer , Epstein Adrienne , Namuganga Jane F. , Kamya Emmanuel V. , Nabende Isaiah , Nassali Martha , Sserwanga Asadu , Gonahasa Samuel , Muwema Mercy , Kiwuwa Steven M. , Staedke Sarah G. , Kamya Moses R. , Nankabirwa Joaniter I. , Briggs Jessica , Jagannathan Prasanna , Dorsey Grant
Published: September 2021

BackgroundRoutine malaria surveillance data in Africa primarily come from public health facilities reporting to national health management information systems. Although information on gender is routinely collected from patients presenting to these health facilities, stratification of malaria surveillance data by gender is rarely done. This study evaluated gender difference among patients diagnosed with laboratory confirmed malaria at public health facilities in Uganda.MethodsThis study utilized individual level patient data collected from January 2020 through April 2021 at 12 public health facilities in Uganda and cross-sectional surveys conducted in target areas around these facilities in April 2021. Associations between gender and the incidence of malaria and non-malarial visits captured at the health facilities from patients residing within the target areas were estimated using poisson regression models controlling for seasonality. Associations between gender and data on health seeking behaviour from the cross-sectional surveys were estimated using poisson regression models controlling for seasonality. ResultsOverall, incidence of malaria diagnosed per 1000 person years was 735 among females and 449 among males (IRR=1.72, 95% CI 1.68-1.77, p<0.001), with larger differences among those 15-39 years (IRR=2.46, 95% CI 2.34-2.58, p<0.001) and over 39 years (IRR=2.26, 95% CI 2.05-2.50, p<0.001) compared to those under 15 years (IRR=1.46, 95% CI 1.41-1.50, p<0.001). Female gender was also associated with a higher incidence of visits where malaria was not suspected (IRR=1.77, 95% CI 1.71-1.83, p<0.001), with a similar pattern across age strata. These associations were consistent across the 12 individual health centres. From the cross-sectional surveys, females were more likely than males to report fever in the past 2 weeks and seek care at the local health centre (7.5% vs 4.7%, p=0.001) with these associations significant for those 15-39 years (RR=2.49, 95% CI 1.17-5.31, p=0.018) and over 39 years (RR=2.56, 95% CI 1.00-6.54, p=0.049). ConclusionsFemales disproportionately contribute to the burden of malaria diagnosed at public health facilities in Uganda, especially once they reach childbearing age. Contributing factors included more frequent visits to these facilities independent of malaria and a higher reported risk of seeking care at these facilites for febrile illnesses.
Study of The Effect of TiO2 Addition On The Crystalline Phases, Structure and Chemical Durability of a Nuclear Glass Ceramic
Authors: SOUAG Rafika , KAMEL Nour elhayet , Moudir Dalila , MOUHEB Yasmina , Aouchiche Fayrouz
Published: September 2021

This study focused on the effect of TiO 2 addition on the crystallines phases’ formation, structure and chemical durability of a nuclear glass ceramic constituted by an aluminosilicate glass in the system: SiO2-Al2O3-CaO-MgO-ZrO2-TiO2 . The materials with four contents of TiO2 , ranging from 4.11 to 7.11 wt.%, are synthesized by a discontinuous method,. For the whole of materials, X-ray diffraction analysis allow identifying an aluminosilicate belonging to pyroxenes silicates family as a main phase, powelite and calzirtite. Both SEM and DTAanalyses confirmed these results. The materials FTIR analysis reveals the glass ceramics complex chemical composition. MCC1 and MCC2 tests, performed on selected glass ceramic materials, indicate that the materials with 4.11 and 5.11 wt.% TiO2 are the most durable against Si, Al, Mg and Ce elements release, in MCC2 test; The results make conclusions valuable on the selection of such glass ceramics as candidate for the disposal of high-level waste.
Utility of Indocyanine Green Videoangiography with FLOW 800 Analysis in Brain Tumor Resection - Venous Protection Technique
Authors: Sun Yue , Wang Zilan , Jiang Fan , Yang Xingyu , Xin Tan , Chen Zhouqing , Liu Yanfei , Zhu Yun , Wang Zhong , Chen Gang
Published: September 2021

Background: When it comes to central nervous system tumor resection, preserving vital venous structures to avoid devastating consequences such as brain edema and hemorrhage is important. Wheras, in clinical practice, it is difficult to obtain clear and vivid intraoperative venous visualization and blood flow analysis.Methods: We presented our clinical cases to demonstrate the process of venous preservation during surgical resection through the application of indocyanine green videoangiography (ICG-VA) integrated with FLOW 800. Galen vein, sylvian vein and superior cerebral veins of the brain were included.Results: Clear documentations of the veins from different venous groups were obtained via ICG-VA integrated with FLOW 800, which semiquantitatively analyzed the flow dynamics. ICG-VA integrated with FLOW 800 enabled us to achieve brain tumor resection without venous injury and obstructing the venous flux.Conclusions: ICG-VA integrated with FLOW 800 is an efficient method for venous preservation, though further comparison between ICG-VA integrated with FLOW 800 and other techniques of intraoperative blood flow monitoring is needed.
Performance evaluation in the reconstruction of 2D images of computed tomography using massively parallel programming CUDA
Authors: Cordeiro Alexssandro Ferreira , Filho Pedro Luiz de Paula , Silva Hamilton Pereira , Junior Arnaldo Candido , Casanova Edresson , Spancerski Jandrei Sartori
Published: September 2021

Purpose: analysis of processing time and similarity of images generated between CPU and GPU architectures and sequential and parallel programming methodologies.

Material and methods: for image processing a computer with AMD FX-8350 processor and an Nvidia GTX 960 Maxwell GPU was used, along with the CUDAFY library and the programming language C# with the IDE Visual studio.

Results: the results of the comparisons indicate that the form of sequential programming in a CPU generates reliable images at a high custom of time when compared to the forms of parallel programming in CPU and GPU. While parallel programming generates faster results, but with increased noise in the reconstructed image. For data types float a GPU obtained best result with average time equivalent to 1/3 of the processor, however the data is of type double the parallel CPU approach obtained the best performance.

Conclusion: for the float data type, the GPU had the best average time performance, while for the double data type the best average time performance was for the parallel approach CPU. Regarding image quality, the sequential approach obtained similar outputs, while theparallel approaches generated noise in their outputs.
Relationship Between Insulin Resistance and Serum Androgens Levels in Women With and Without PCOS by Rotterdam Diagnosis Criteria
Authors: Movahedinejad Maryam , Ziaei Saeide , Kazemnezhad Anooshiravan , kamali zahra
Published: September 2021

IntroductionPCOS is the most common endocrine disorder in women of childbearing age. Insulin resistance is a key component of the pathophysiology of PCOS that can increase the secretion of serum androgens. The aim of this study was to determine the relationship between insulin resistance and serum androgens levels in PCOS subgroups.MethodsHormonal and clinical laboratory studies were performed for all research units. Insulin resistance was diagnosed with the HOMA index (Cut off> 2.5). ResultsIn subgroup A, there was a positive relationship between HOMA-IR with FAI (P = 0.014) and negative relationship with SHBG (P <0.001). In subgroup C, a negative relationship was observed between HOMA-IR and SHBG (P = 0.002). In subgroup D, there was a positive relationship between HOMA-IR and FAI (P = 0.035). No significant association was found in the control group and subgroup B (P> 0.05). Also, in all different PCOS phenotypes, no association was found between HOMA-IR and serum TT levels (P> 0.05).ConclusionThere was a relationship between insulin resistance and some serum androgens in PCOS subtypes. Therefore, it is hoped that early detection of these at-risk patients will be done through early biochemical markers to prevent and change lifestyle.
Differentially Expressed Circular RNAs in Stenosed Arteriovenous Fistula Tissues of Uremia Patients
Authors: Lan Lili , Liu Yu , Zou Menglin , Xie Yihang , Zhang Yiye , Tian Liqing , Xiao Jun , Yang Liu , Zhang Li , Yan Yan
Published: September 2021

BackgroundArteriovenous fistula (AVF) is the most common renal replacement therapy for uremic patients. However, stenosis in AVF may lead to AVF failure, hence prevention and effective management of AVF failure is an issue to be addressed. circular RNAs (circRNAs) dysregulation may be pivotal for the development and progression of AVF stenosis. MethodsFour stenosed tissues from AVF outflow veins and four normal venous tissues without vascular stenosis were collected for RNA-sequencing (RNA-seq). The circRNAs expression profiles were identified by high-throughput sequencing, and the functions and pathways of differentially expressed (DE) circRNAs were annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Gene Genomes (KEGG) enrichment analyses. Seven DE circRNAs were screened for quantitative real‐time polymerase chain reaction (qRT-PCR) validation. circRNA-miRNA interaction network was constructed. ResultsA total of 17,620 circRNA transcripts were examined by RNA-seq, and 208 DE circrRNAs were identified between AG and CG, of which 92 were upregulated and 116 were downregulated. The expression trend in the four selected circRNAs was validated by qRT-PCR, which was consistent with the RNA-seq results. Dysregulated circRNAs may be involved in stenosis by mediating focal adhesion kinase (FAK) pathway. Conclusion﻿Our study revealed abnormal circRNA expression in stenosed tissues of the AVF outflow vein, which was functionally classified. The results indicated that DE circRNAs in the stenosed tissues of AVF and their related FAK pathway have potential to be targets for the prevention and treatment of AVF failure.
Effect of Dietary Zinc Supplementation on the Gastrointestinal Microbiota and Host Gene Expression in the Shank3B-/- Mouse Model of Autism Spectrum Disorder
Authors: Wong Giselle C , Jung Yewon , Lee Kevin , Fourie Chantelle , Handley Kim M. , Montgomery Johanna M , Taylor Michael W.
Published: September 2021

Background: Shank genes are implicated in ~1% of people with autism and mice with Shank3 knock out mutations exhibit autism-like behaviours. Zinc deficiency and gastrointestinal problems can be common among people with autism, and zinc is a key element required for SHANK protein function and gut development. In Shank3B-/- mice, a supplementary zinc diet reverses autism behaviours. We hypothesise that dietary zinc may alter the gut microbiome, potentially affecting the gut-microbiota-brain axis, which may contribute to changes in autism-like behaviours. Methods: Four types of gastrointestinal samples (ileum, caecum, colon, faecal) were collected from wild-type and knock-out Shank3B-/- mice on either control or supplemented-zinc diets. Bacterial 16S rRNA gene and fungal ITS2 genomic region amplicons were sequenced on the Illumina MiSeq platform and RNA on the Illumina HiSeq platform.Results: Cage, genotype and zinc diet each contributed significantly to bacterial community variation (accounting for 12.8%, 3.9% and 2.3% of the variation, respectively). Fungal diversity differed significantly between wild-type and knock-out Shank3B-/- mice on the control zinc diet, and the fungal biota differed among gut locations. RNA-seq analysis of host (mouse) transcripts revealed differential expression of genes involved in host metabolism that may be regulated by the gut microbiota and genes involved in anti-microbial interactions. Limitations: This study used the Shank3B-/- mouse model of autism spectrum disorder. Heterozygous and homozygous Shank3 gene mutations are found in 1% of the ASD population, only homozygous Shank3 mice were utilised in this study. Any translational conclusions should consider these limitations.Conclusions: By utilising the Shank3B-/- knock-out mouse model we were able to examine the influence of – and interactions between – dietary zinc and ASD-linked host genotype. Differential expression of host antimicrobial interaction genes as well as gut microbiota-regulated host metabolism genes among the treatment groups, suggests that the interplay between gut microbes, the gastrointestinal tract and the brain may play a major role towards the observed amelioration of ASD behaviours seen previously with supplemented dietary zinc. These data broaden understanding of the gut microbiome in autism and pave the way towards potential microbial therapeutics for gastrointestinal problems in people with autism.
Short-Term Effects of Ambient Air Pollution On Daily Emergency Room Visits for Abdominal Pain: A Time-Series Study in Wuhan, China
Authors: Liu Yaqi , Jiang Yi , Wu Manyi , Muheyat Sunghar , Yao Dongai , Jin Xiaoqing
Published: September 2021

Background

There are few studies focused on the correlations between ambient air pollution and abdominal pain, especially in emergency departments in China.
Method:

Daily data (from January 1, 2016 to December 31, 2018), including air pollution concentration (SO2, NO2, PM2.5, PM10, CO, and O3) and meteorological variables, for daily emergency room visits (ERVs) were collected in Wuhan, China. We conducted a time-series study to investigate the potential correlation between six ambient air pollutants and ERVs for abdominal pain and their effects, in different genders, ages and seasons.
Results

A total of 16,306 abdominal pain ERVs were identified during the study period. A 10-µg/m3 increase in concentration of SO2, NO2, PM2.5, PM10, CO, and O3 corresponded respectively to incremental increases in abdominal pain of 6.12% (95% confidence interval [CI]: -0.44-13.12), 1.65% (95%CI: -0.25-3.59), 1.12% (95%CI: -0.18-2.44), 0.38% (95%CI: -1.09-1.87), 9.87% (95%CI:3.14–17.05) and 1.11% (95%CI: 0.03–2.21). We observed significant correlations between CO and O3 and daily abdominal pain ERVs increase, and positive but insignificant correlations between the other pollutants and ERVs. The effects were stronger mainly for females (especially SO2 and O3) and younger people (especially CO and O3). The correlations of PM2.5 and PM10 were stronger in cool seasons, while the correlation of CO was stronger in warm seasons.
Conclusion

Our time-series study suggested that short-term exposure to air pollution (especially CO and O3) was positively correlated with ERVs for abdominal pain in Wuhan, China, and that their effects varied by season, gender and age. These data can add evidence on how air pollutants affect the human body, and may prompt hospitals to take specific precautions on polluted days and maintain order in emergency departments made busier due to the pollution.
Design Optimization of Nanotube Tunnel Field-Effect Transistor with Bias-Induced Electron-Hole Bilayer
Authors: Wang Xueke , Sun Yabin , Liu Ziyu , Liu Yun , Li Xiaojin , Shi Yanling
Published: September 2021

In this paper, a novel nanotube tunneling field-effect transistor (NT-TFET) with bias-induced electron-hole bilayer (EHBNT-TFET) is proposed for the first time. By the intentional misalignment and an asymmetric bias configuration of the inner-gate and outer-gate, the line tunneling takes place inside the channel, significantly improving the tunneling rate and area. The device principle and performance are investigated by calibrated 3-D TCAD simulations. Compared to the conventional NT-TFET, the proposed EHBNT-TFET exhibits an increased ON-state current (ION) about 57.2 times and a sub-60 mV/dec subthreshold swing for seven orders of magnitude of drain current. Furthermore, the increased ION and reduced gate capacitance achieve improved dynamic performance. Compared with conventional NT-TFET, the intrinsic delay decreased about 142 times is obtained in EHBNT-TFET.
Effects of Xuezhikang vs. Pravastatin on Triglyceride Level in Patients with Type 2 Diabetes Mellitus and Dyslipidemia: A Research Protocol for a Randomized Controlled Study
Authors: Xu Jin , Du Xiao , Zhang Shilan , Xiang Qunyan , Guo Liling , Zhu Liyuan , Tan Yangrong , Fu Yan , Xie Yingying , Zhang Miao , Wen Tie , Liu Ling
Published: September 2021

Background: Diabetes often accompanies with increase in triglyceride(TG) as well as small dense low density lipoprotein(sdLDL). Statins are difficult to completely correct this dyslipidemia. Xuezhikang, an extract of cholestin, is better than some statins in reducing TG. Under the condition of the similar decrease in LDL-cholesterol (LDL-C), it is not clear whether there is any difference in the effect of XZK and statins on TG reduction in patients with Type 2 DM(T2DM). Methods: An open-label multicenter study is planned to estimate the effects of Xuezhikang(1.2 g/d) and pravastatin(20 mg/d) on TG level and other blood lipids in T2DM patients with dyslipidemia and moderate to high risk of cardiovascular diseases. A total of 114 patients will be enrolled and randomly assigned (1:1 ratio) to accept Xuezhikang or pravastatin therapy for 6 weeks. The primary outcome measure is the change in fasting TG level after 6 weeks. The changes in other fasting lipids and postprandial lipids at 1, 2, 4 h after a nutritious breakfast will also be explored. The planned duration for enrollment is between November 2021 and December 2022. Conclusion: This study will evaluate the effect of 6-week treatment of Xuezhikang(1.2 g/d) and pravastatin(20 mg/d) on fasting TG level and other blood lipids in T2DM patients with dyslipidemia, which may provide a more optimized schedule for lipid control in patients with diabetes and dyslipidemia in primary prevention.
Risk Factors and Clinical Characteristics of Catheter Line-associated Bloodstream Infection in MIMIC IV Database
Authors: Li Fang , Lu Fan , Zhang Luming , Li Longzhu , Xing Mingyao , Shen Tao , Yin Haiyan , Lyu Jun
Published: September 2021

Background: Central line-associated bloodstream infection (CLABSI) is a common hospital infection. The increasing use of peripherally inserted central catheters and fully implanted venous ports, data on the epidemiological and clinical characteristics of CLABSI in the overall central venous catheter (CVC) population remain limited. The Medical Information Mart for Intensive Care (MIMIC IV) database is a free, open, and public resource research database. The purpose of this study was to describe the risk factors and clinical characteristics of CLABSI in MIMIC IV.Methods: A total of 31,116 patients were included in this study. General information, CVC-related information, comorbidity information, microbiological information, and antibiotic use information were extracted to describe and analyze the clinical characteristics of patients with CLABSI.Results: According to the occurrence of CLABSI, 31,116 patients were divided into the NO CLABSI group (n=30,395) and CLABSI group (n=721). The total indwelling duration of CVC was 439,239.6 days, The incidence of CLABSI is 2.32% and 1.64/1000 catheter days. The risk factors for CLABSI were the number of CVC type, duration of CVC, number of antibiotic type, duration of antibiotics, and femoral vein and internal jugular vein intubation. The in-hospital mortality of the CLABSI group was higher than that of the NO CLABSI group, but no statistical significance was observed (P>0.05). Gram-positive (G+) cocci and Gram-negative (G-) bacilli accounted for 80% and 16.93% of positive bacteria in catheter culture. G+ cocci and G- bacilli accounted for 59.45% and 25.62% of positive bacteria in blood culture. Drugs with the highest resistance rates in catheter culture included penicillin G benzathine, oxacillin, and erythromycin. The most commonly used antibiotics for the treatment of CLABSI included vancomycin, cefepime, piperacillin tazobactam, and cefazolin.Conclusions: This study investigated independent risk factors for CLABSI and their association with in-hospital mortality and described the etiological characteristics, drug sensitivity, and the distribution of antibiotics used for treatment.
SINE Insertion in 5’ Flanking of Porcine IGFBP3 May Associated with Gene Expression and Phenotypic Variations
Authors: wang xiaoyan , An Yalong , Murani Eduard , D'alessandro Enrico , Chi Chengling , Chen Cai , Moawad Ali Shoaib , Li Kui , Wimmers Klaus , Song Chengyi
Published: September 2021

Background: Insulin-like growth factor binding proteins (IGFBPs) have been considered important candidate genes for economic traits due to their involvement in physiological processes related to growth and development. However, most of the current studies on genetic markers of IGFBPs have focused on SNPs, and large fragment insertion mutations such as retrotransposons have rarely been considered.Results: In total twelve retrotransposon insertion polymorphisms (RIPs) were confirmed using bioinformatics prediction combined with the PCR-based amplification. By linkage genetic analysis, IGFBP3-1-RIP and IGFBP3-2-RIP are completely linked, showing only three genotypes, SINE+/+/LINE-/-, SINE-/-/LINE+/+ and SINE+/-/LINE-/+. The age of 100 kg body weight and longissimus muscle thickness of Large white individuals of SINE+/+/LINE-/-genotype were significantly (P<0.05) higher than those of other two genotypes. However, corrected backfat thickness of SINE+/+/LINE-/- individuals were significantly (P<0.05) thinner than those of SINE+/+/LINE-/- genotype. The expression of the IGFBP3 gene in liver and backfat of 30-day Sujiang piglets with SINE+/+/LINE-/- genotype were significantly higher (P<0.05) than those with SINE-/-/LINE+/+ genotype by qPCR. After the core promoter region of the IGFBP3 gene was identified locating within 482bp upstream of ATG by using the dual-luciferase activity assay, further study was conducted to confirm the effect of SINE of IGFBP3-1-RIP and LINE of IGFBP3-2-RIP on the promoter activity of IGFBP3 based on the PGL3-Promoter-Enhancer. The result revealed that only SINE insertion was significantly increased (P<0.05) promoter activity of the IGFBP3 gene, indicating that the SINE may act as an enhancer to regulate the promoter activity of the IGFBP3 gene.Conclusions: Overall, this study identified 12 RIPs in IGFBP gene clusters. Furthermore, SINE insertions in 5’ flanking of IGFBP3 may associated with variations of gene expression and phenotype.
Novel Oral Anticoagulant Use in Adults With Congenital Heart Disease: a Single-center Experience
Authors: Samarai Daniel , Isma Nazim , Lindstedt Sandra , Hlebowicz Joanna
Published: September 2021

IntroductionAdults with congenital heart disease (ACHD) are a group with an increased risk of thromboembolic complications and arrhythmias. Vitamin K antagonists (VKA) are the most commonly used thromboprophylaxis therapy in this population. Studies on the efficacy and safety of novel oral anticoagulants (NOAC) are scare, but emerging together with their increasing use.MethodsACHD patients taking NOAC treatment were identified in AuriculA, a Swedish national quality register for atrial fibrillation and anticoagulation. Data on duration of treatment and patient characteristics were provided by the Register. CHA2DS2-VASc and HAS-BLED scores for atrial fibrillation were calculated. CHD severity was determined according to guidelines. Thromboembolic and major bleeding events were provided by AuriculA. Results30 patients who had been taking NOAC treatment for a minimum of 3 months were included. Their median age was 55 years (SD 17 years) and 57% were male. Median follow-up was 17 months (IQR: 10-41). Apixaban was the most commonly used NOAC (47%). Median CHA2DS2-VASc score was 2 (IQR: 0-3) and HAS-BLED was 1 (IQR: 0-2). Complex CHD was prevalent in 27% of the patients. No thromboembolic events were recorded; however, one major bleeding, unspecified, was reported during the total cumulative patient follow-up time of 64 years. ConclusionThe results of our study, although limited in size, suggest NOAC to be a non-inferior alternative to VKA in a heterogenic study group with a balanced inclusion of CHD severity defects. Further and larger studies on VKA and NOAC in ACHD patients are warranted.
Superconductivity modulated by structural phase transitions in pressurized vanadium-based kagome metals
Authors: Du Feng , Li Rui , Luo Shuaishuai , Gong Yu , Li Yanchun , Jiang Sheng , Ortiz Brenden , Liu Yi , Xu Xiaofeng , Wilson Stephen , Cao Chao , Song Yu , Yuan Huiqiu
Published: September 2021

The interplay of superconductivity with electronic and structural instabilities on the kagome lattice provides a fertile ground for the emergence of unusual phenomena. The vanadium-based kagome metals AV3Sb5 (A = K, Rb, Cs) exhibit superconductivity on an almost ideal kagome lattice, with the superconducting transition temperature Tc forming two domes upon pressure-tuning. The first dome arises from the competition between superconductivity and a charge-density-wave, whereas the origin for the second dome remains unclear. Herein, we show that the appearance of the second superconducting dome in KV3Sb5 and RbV3Sb5 is associated with transitions from hexagonal P6/mmm to monoclinic P2/m structures, evidenced by splitting of structural peaks from synchrotron powder X-ray diffraction experiments and imaginary phonon frequencies in first-principles calculations. In KV3Sb5, transition to an orthorhombic Pmmm structure is further observed for pressure p≥20 GPa, and is correlated with the strong suppression of Tc in the second superconducting dome. Our findings indicate distortions of the crystal structure modulates superconductivity in AV3Sb5 under pressure, providing a platform to study the emergence of superconductivity in the presence of multiple structural instabilities.
Long-Chain Alkenones in the Shimosa Group Reveal Palaeotemperatures of the Pleistocene Interglacial Palaeo-Tokyo Bays
Authors: Kajita Hiroto , Nakazawa Tsutomu , Ohkouchi Naohiko , Sato Miyako , Harada Naomi , Kawahata Hodaka
Published: September 2021

The Shimosa Group, middle- to late-Pleistocene sedimentary succession, has been the focus of stratigraphic attention because it is beneath the Tokyo metropolitan area of central Japan. It is also of palaeoclimatic significance because it contains important interglacial marine strata of the past 450,000 years. Since the marine strata of the Shimosa Group were formed in the fluvial, estuary, and shallow inner bay known as Palaeo-Tokyo Bay, few occurrences of marine microfossils, make it difficult to quantitatively reconstruct the palaeotemperatures. Here, we extracted long-chain alkenones from the core GS-UR-1 penetrating the Shimosa Group to Marine Isotope Stage (MIS) 11. We found that the alkenone unsaturation ratio appears to reflect the sea surface temperatures (SSTs) of Palaeo-Tokyo Bays formed during MIS 5e, 7e, 9, and 11, which might be recorded around the peak of each interglacial period. The palaeo-SSTs during each interglacial period were 2–3 ℃ higher than the palaeo-SSTs of Tokyo Bay in the pre-industrial era, seemed to reach the similar level as the Holocene thermal maximum. We suggest that the LCA-based proxy, which has not been utilized hitherto in studies on the Shimosa Group, demonstrates its potential to provide palaeoclimatic and stratigraphic information.
Time-Resolved Detection of Multilevel Switching of the Magnetization and Exchange Bias Driven by Spin-Orbit Torques
Authors: Wang Yuyan , Taniguchi Takuya , Lin Po-Hung , Zicchino Daniel , Nickl Andreas , Sahliger Jan , Lai Chih-Huang , Song Cheng , Wu Huaqiang , Dai Qionghai , Back Christian
Published: September 2021

Current induced magnetization switching, jointly with the manipulation of exchange bias, via spin-orbit torques (SOT) on sub-nanosecond timescales hold great promise for fast and low-power spintronic devices. Specifically, the time-resolved detection and subsequent analysis of switching trajectories relevant to ferromagnet/antiferromagnet exchange biased structures are central to designing SOT devices with high speed, and are still open questions. Here, we report the SOT-induced multileveled switching on sub-nanosecond timescales in Pt/Co/IrMn heterostructures, and illustrate the time-resolved magnetization switching trajectories of the exchange bias. By adopting time-resolved magneto-optical Kerr microscopy combined with micromagnetic simulations, our work reveals that not only the ferromagnets, but also the multiple antiferromagnetic domains and exchange bias, can be partially switched by sub-nanosecond current pulse, to flexibly control the switching probabilities at multiple levels. The experiments demonstrate that the SOT switching of exchange bias, which immediately depends on the current density, can significantly stabilize the multileveled magnetization switching within sub-nanosecond current pulse with high thermal stability.