bluuebunny/crossref_metadata_2025 huggingface dataset:
Minimally invasive Ivor Lewis oesophagectomy with trans-hiatal oesophageal transection and transabdominal specimen extraction for Siewert II oesophagogastric cancer
Authors: Dalmonte G , Valente M , Tartamella F , Cecconi S , Annicchiarico A , Marchesi F
Published: April 2022

The optimal surgical procedure for Siewert II oesophagogastric junction cancer is still debated. The minimally invasive Ivor Lewis technique can be considered the most adequate intervention from the oncological perspective but it is still contested owing to its technical difficulties. To allow an easier thoracoscopic stage during the procedure, we performed it with laparoscopic trans-hiatal oesophageal transection and transabdominal extraction. An 80-year-old man with stage 3 Siewert II oesophagogastric junction adenocarcinoma not suitable for neoadjuvant therapy underwent minimally invasive Ivor Lewis oesophagectomy with two-field lymphadenectomy, using a laparoscopic and thoracoscopic approach in prone position. The trans-hiatal oesophageal resection permitted easy extraction of a transabdominal specimen and frozen section examination. The prone position, together with the absence of the specimen in the operative field, allowed easier mediastinal node dissection and oesophagogastric anastomosis with better visualisation. The postoperative course was uneventful. Pathology showed a G3–pT3, N2 adenocarcinoma with 6/30 metastatic lymph nodes.
Composição de amebas testáceas (Amoebozoa: Rhizopoda) associadas à rizosfera de Eichhornia crassipes (Martius) Solomons (Pontederiaceae) no Rio Cachoeira, Bahia, Brasil: novos registros para o Nordeste
Authors: Silva Márcio Borba da , Ribeiro Sylvia Maria M. S. , Velho Luiz Felipe Machado
Published: April 2022

Studies on microorganisms associated or adhered to aquatic macrophytes are scarce. Due to the important role that testate amoebae (Amoebozoa: Rhizopoda) have in the metabolism of inland waters and the scarcity of information about diversity of this group, the objective of this study was to characterize the composition of testate amoebae associated with Eichhornia crassipes stands in this river, in two hydrologic periods. This work registered 115 taxa of testate amoebae associated to the rizosphere of E. crassipes, belonging to 10 families, 14 genera, 96 species and 19 varieties, distributed in Difflugiidae (42 taxa), Arcellidae (21 taxa), Centropyxidae (14), Euglyphidae, Lesquereusiidae (11 each), Cryptodifflugiidae (7), Trigonopyxidae, Trinematiidae (3 each), Hyalospheniidae (2), and Cyphoderiidae (1). The high richness of testate amoebae are registered in the flood periods river. However, high similarity was observed within the assemblage composition in two hydrological periods. This work includes 84 new registers of tecamebas for the Northeast of Brazil, thus totalizing 137 taxa for this region, and for Bahia State it was recorded 112 new occurrences, what demonstrates the necessity to increase the efforts of collection in the state.
Relationship Between Sensory Processing and Autism Spectrum Disorder-Like Behaviors in Prader-Willi Syndrome
Authors: Saima Sohei , Ihara Hiroshi , Ogata Hiroyuki , Gito Masao , Murakami Nobuyuki , Oto Yuji , Ishii Atsushi , Takahashi Asami , Nagai Toshiro
Published: April 2022

The relationship between sensory processing and ASD-like and associated behaviors in patients with Prader-Willi Syndrome (PWS) remains relatively unexplored. Examining this relationship, 51 adults with PWS were administered the Pervasive Developmental Disorders Autism Society Japan Rating Scale (PARS), Short Sensory Profile (SSP-J), Food-Related Problem Questionnaire (FRPQ), and Aberrant Behavior Checklist (ABC-J). Based on SSP-J z-scores, participants were classified into three severity groups. Analysis of variance was performed to compare the behavioral scores of these three groups. Statistically significant group differences were observed in PARS (p = .006, ηp2 = .194) and ABC-J (p = .006, ηp2 = .193) scores. Our findings suggest that the level of sensory processing may predict ASD-like and aberrant behaviors in adults with PWS, implying the importance of a proper assessment for early intervention.
Subsurface phytoplankton vertical structure from lidar observation during SCS summer monsoon onset
Authors: Zhang Siqi , Chen Peng
Published: April 2022

Subsurface phytoplankton vertical structure was observed for the first time by lidar during the onset of the SCS summer monsoon. Based on the lidar data that were obtained by continuous day-and-night measurements over a two-week period, a hybrid retrieval method to determine the vertical structure of the seawater chlorophyll-a concentrations using lidar data was proposed. We compared the data obtained from the lidar retrievals with the ocean color data and studied the spatial variations and hourly diurnal variations in the subsurface chlorophyll-a maximum layer (SCML). The significant changes in the depth of the SCML in the SCS may be due to the variations in light availability and nutrient supply during the onset of the SCS summer monsoon. The preliminary results indicated that lidar measurements allow the submesoscale oceanic dynamics mechanisms to be understood from a new perspective.
Quantitative phase imaging based on model transfer learning
Authors: Chen Jiawei , Zhang Qinnan , Lu Xiaoxu , Zhong Liyun , Tian Jindong
Published: April 2022

Convolutional neural networks have been widely used in optical information processing and the generalization ability of the network depends greatly on the scale and diversity of the datasets, however, the acquisition of mass datasets and later annotation have become a common problem that hinders its further progress. In this study, a model transfer-based quantitative phase imaging (QPI) method is proposed, which fine-tunes the network parameters through loading pre-training base model and transfer learning, enable the network with good generalization ability. Most importantly, a feature fusion method based on moment reconstruction is proposed for training dataset generation, which can construct rich enough datasets that can cover most situations and accurately annotated, it fundamentally solves the problem from the scale and representational ability of the datasets. Besides, a feature distribution distance scoring (FDDS) rule is proposed to evaluate the rationality of the constructed datasets. The experimental results show that this method is suitable for different types of samples to achieve fast and high-accuracy phase imaging, which greatly relieves the pressure of data, tagging and generalization ability in the data-driven method.
Polarimetric fiber laser for relative humidity sensing based on graphene oxide-coated D-shaped fiber and beat frequency demodulation
Authors: Guo Kuikui , He Jun , Li He , Xu Xizhen , Du Bin , Liu Shen , Chen Yanping , Ma Dingbang , Wang Ying , Xu Gaixia , Wang Yiping
Published: April 2022

We first propose and demonstrate a polarimetric fiber laser system for relative humidity (RH) sensing based on the beat frequency demodulation. A graphene oxide-coated D-shaped fiber (GDF) with a low insertion loss of 0.8 dB was embedded into a laser cavity to form an RH sensing probe. The output of the fiber laser could generate mode splitting between two orthogonal polarization modes due to birefringence of the GDF device. Hence, two types of beat signals, i.e., longitudinal mode beat frequency (LMBF) and polarization mode beat frequency (PMBF) could be generated synchronously. The experimental results indicated that the LMBFs of the fiber laser had almost no response to the ambient humidity, and the PMBFs of the fiber laser were very sensitive to the various RH levels. There was a good linear relationship between the PMBF and RH changes in the range of 30% to 98%. This fiber-optic RH sensor exhibited a sensitivity of 34.7 kHz/RH% with a high quality of fit (R2>0.997) during the ambient RH increase and decrease. Moreover, the average response and recovery times of the fiber-optic RH sensor were measured to be about 64.2 ms and 97.8 ms, respectively. Due to its long stability, reversibility, quick response time and low temperature cross-sensitivity (i.e., 0.12 RH%/°C), the proposed fiber-optic RH sensor could offer attractive applications in many fields, such as biology, chemical processing and food processing, etc.
Genetic algorithm-powered non-sequential dwell time optimization for large optics fabrication
Authors: Kang Hyukmo , Wang Tianyi , Choi Heejoo , Kim Daewook
Published: April 2022

Computer Controlled Optical Surfacing (CCOS) is widely applied for fabricating large aspheric optical surfaces. For large optics fabrication, various sizes of polishing tools are used sequentially. This raises the importance of efficient and globally optimized dwell time map of each tool. In this study, we propose a GEnetic Algorithm-powered Non-Sequential (GEANS) optimization technique to improve the feasibility of the conventional non-sequential optimization technique. GEANS consists of two interdependent parts: i) compose an influence matrix by imposing constraints on adjacent dwell points and ii) induce the desired dwell time map through the genetic algorithm. CCOS simulation results show that GEANS generates a preferable dwell time map that provides high figuring efficiency and structural similarity with the shape of target removal map, while improving computational efficiency more than 1000 times over the conventional non-sequential optimization method. The practicability of GEANS is demonstrated through error analyses. Random tool positioning error and tool influence function errors are imposed on dwell time maps. Compared to the conventional non-sequential optimization method, the power spectral density values of residual surface error from GEANS remain stable. GEANS also shows superior applicability when the maximum acceleration of a tool is applied.
Injection-seeded 10 kHz repetition rate Er:YAG solid-state laser with single-frequency pulse energy more than 1 mJ
Authors: Chen Chaoyong , Gao Chunqing , Wang Qing
Published: April 2022

We report a single-frequency Q-switched Er:YAG all-solid-state laser with a pulse repetition rate of up to 10 kHz. The single-frequency feature is ensured by injecting the seed laser into a Q-switched ring cavity, and the pulse repetition rate is increased by combing the Pound-Drever-Hall method and optical feedback. Peak power of 4.12 kW with an average pulse energy of 1.35 mJ single-frequency 1645 nm laser pulses is achieved at a pulse repetition rate of 10 kHz, which matches an average power of 13.5 W.
Application of modular response analysis to medium- to large-size biological systems
Authors: Mekedem Meriem , Ravel Patrice , Colinge Jacques
Published: April 2022

The development of high-throughput genomic technologies associated with recent genetic perturbation techniques such as short hairpin RNA (shRNA), gene trapping, or gene editing (CRISPR/Cas9) has made it possible to obtain large perturbation data sets. These data sets are invaluable sources of information regarding the function of genes, and they offer unique opportunities to reverse engineer gene regulatory networks in specific cell types. Modular response analysis (MRA) is a well-accepted mathematical modeling method that is precisely aimed at such network inference tasks, but its use has been limited to rather small biological systems so far. In this study, we show that MRA can be employed on large systems with almost 1,000 network components. In particular, we show that MRA performance surpasses general-purpose mutual information-based algorithms. Part of these competitive results was obtained by the application of a novel heuristic that pruned MRA-inferred interactionsa posteriori. We also exploited a block structure in MRA linear algebra to parallelize large system resolutions.
Multiscale affinity maturation simulations to elicit broadly neutralizing antibodies against HIV
Authors: Conti Simone , Ovchinnikov Victor , Faris Jonathan G. , Chakraborty Arup K. , Karplus Martin , Sprenger Kayla G.
Published: April 2022

The design of vaccines against highly mutable pathogens, such as HIV and influenza, requires a detailed understanding of how the adaptive immune system responds to encountering multiple variant antigens (Ags). Here, we describe a multiscale model of B cell receptor (BCR) affinity maturation that employs actual BCR nucleotide sequences and treats BCR/Ag interactions in atomistic detail. We apply the model to simulate the maturation of a broadly neutralizing Ab (bnAb) against HIV. Starting from a germline precursor sequence of the VRC01 anti-HIV Ab, we simulate BCR evolution in response to different vaccination protocols and different Ags, which were previously designed by us. The simulation results provide qualitative guidelines for future vaccine design and reveal unique insights into bnAb evolution against the CD4 binding site of HIV. Our model makes possible direct comparisons of simulated BCR populations with results of deep sequencing data, which will be explored in future applications.
A modified TurboID approach identifies tissue-specific centriolar components in C. elegans
Authors: Holzer Elisabeth , Rumpf-Kienzl Cornelia , Falk Sebastian , Dammermann Alexander
Published: April 2022

Proximity-dependent labeling approaches such as BioID have been a great boon to studies of protein-protein interactions in the context of cytoskeletal structures such as centrosomes which are poorly amenable to traditional biochemical approaches like immunoprecipitation and tandem affinity purification. Yet, these methods have so far not been applied extensively to invertebrate experimental models such as C. elegans given the long labeling times required for the original promiscuous biotin ligase variant BirA*. Here, we show that the recently developed variant TurboID successfully probes the interactomes of both stably associated (SPD-5) and dynamically localized (PLK-1) centrosomal components. We further develop an indirect proximity labeling method employing a GFP nanobody-TurboID fusion, which allows the identification of protein interactors in a tissue-specific manner in the context of the whole animal. Critically, this approach utilizes available endogenous GFP fusions, avoiding the need to generate multiple additional strains for each target protein and the potential complications associated with overexpressing the protein from transgenes. Using this method, we identify homologs of two highly conserved centriolar components, Cep97 and BLD10/Cep135, which are present in various somatic tissues of the worm. Surprisingly, neither protein is expressed in early embryos, likely explaining why these proteins have escaped attention until now. Our work expands the experimental repertoire for C. elegans and opens the door for further studies of tissue-specific variation in centrosome architecture.
Enhanced Integration of TB Services in Reproductive Maternal Newborn and Child Health (RMNCH) Settings in Eswatini
Authors: Hartsough Kieran , Teasdale Chloe A. , Shongwe Siphesihle , Geller Amanda , Pimentel De Gusmao Eduarda , Dlamini Phumzile , Mafukidze Arnold , Pasipamire Munyaradzi , Ao Trong , Ryan Caroline , Modi Surbhi , Abrams Elaine J. , Howard Andrea A.
Published: April 2022

Tuberculosis (TB) primarily affects women during their reproductive years and contributes to maternal mortality and poor pregnancy outcomes. For pregnant women living with HIV (WLHIV), TB is the leading cause of non-obstetric maternal mortality, and pregnant WLHIV with TB are at increased risk of transmitting both TB and HIV to their infants. TB diagnosis among pregnant women, particularly WLHIV, remains challenging, and TB preventive treatment (TPT) coverage among pregnant WLHIV is limited. This project aimed to strengthen integrated TB and reproductive, maternal, neonatal and child health (RMNCH) services in Eswatini to improve screening and treatment for TB disease, TPT uptake and completion among women receiving RMNCH services. The project was conducted from April-December 2017 at four health facilities in Eswatini and introduced enhanced monitoring tools and on-site technical support in RMNCH services. We present data on TB case finding among women, and TPT coverage and completion among eligible WLHIV. A questionnaire (S1 Appendix) measured healthcare provider perspectives on the project after three months of project implementation, including feasibility of scaling-up integrated TB and RMNCH services. A total of 5,724 women (HIV-negative or WLHIV) were screened for active TB disease while attending RMNCH services; 53 (0.9%) were identified with presumptive TB, of whom 37 (70%) were evaluated for TB disease and 6 (0.1% of those screened) were diagnosed with TB. Among 1,950 WLHIV who screened negative for TB, 848 (43%) initiated TPT and 462 (54%) completed. Forty-three healthcare providers completed the questionnaire, and overall were highly supportive of integrated TB and RMNCH services. Integration of TB/HIV services in RMNCH settings was feasible and ensured high TB screening coverage among women of reproductive age, however, symptom screening identified few TB cases, and further studies should explore various screening algorithms and diagnostics that optimize case finding in this population. Interventions should focus on working with healthcare providers and patients to improve TPT initiation and completion rates.
Diverse and atypical manifestations of Q fever in a metropolitan city hospital: Emerging role of next-generation sequencing for laboratory diagnosis of Coxiella burnetii
Authors: Xing Fanfan , Ye Haiyan , Deng Chaowen , Sun Linlin , Yuan Yanfei , Lu Qianyun , Yang Jin , Lo Simon K. F. , Zhang Ruiping , Chen Jonathan H. K. , Chan Jasper F. W. , Lau Susanna K. P , Woo Patrick C. Y.
Published: April 2022

Although Q fever has been widely reported in the rural areas of China, there is a paucity of data on the epidemiology and clinical characteristics of this disease in large metropolitan cities. In this study, we profile the epidemiology and clinical manifestations of Q fever from a tertiary hospital in Shenzhen, a Southern Chinese metropolitan city with a large immigrant population from other parts of China. A total of 14 patients were confirmed to have Q fever during a nine-year-and-six-month period, five of whom were retrospectively diagnosed during case review or incidentally picked up because of another research project on unexplained fever without localizing features. Some patients had the typical exposure histories and clinical features, while a few other patients had rare manifestations of Q fever, including one with heart failure and diffuse intracapillary proliferative glomerulonephritis, a patient presenting with a spontaneous bacterial peritonitis-like syndrome, and another one with concomitant Q fever and brucellosis. Using a combination of clinical manifestation, inflammatory marker levels, echocardiographic findings and serological or molecular test results, nine, three and two patients were diagnosed to have acute, chronic and convalescent Q fever, respectively. Seven, five and two patients were diagnosed to have Q fever by serological test, nested real-time PCR and next-generation sequencing respectively. Diverse and atypical manifestations are associated with Q fever. The incidence of Q fever is likely to be underestimated. Next-generation sequencing is becoming an important diagnostic modality for culture-negative infections, particularly those that the physicians fail to recognize clinically, such as Q fever.
Contextualizing mobility during the Ebola epidemic in Liberia
Authors: Fallah Mosoka , Lavalah Stephen , Gbelia Tina , Zondo Myers , Kromah Morris , Tantum Lucy , Nallo Gartee , Boakai Joseph , Sheriff Kemoh , Skrip Laura , Ali S. Harris
Published: April 2022

Based on findings from focus groups and key informant interviews conducted at five sites in Liberia between 2018 and 2019, we explore some of the key factors that influenced people’s motivation to travel during the 2014–2016 Ebola Virus Disease (EVD). We discuss how these factors led to certain mobility patterns and the implications these had for EVD response. The reasons for individual mobility during the epidemic were multiple and diverse. Some movements were related to relocation efforts as people attempted to extricate themselves from stigmatizing situations. Others were motivated by fear, convinced that other communities would be safer, particularly if extended family members resided there. Individuals also felt compelled to travel during the epidemic to meet other needs and obligations, such as attending burial rites. Some expressed concerns about obtaining food and earning a livelihood. Notably, these latter concerns served as an impetus to travel surreptitiously to evade quarantine directives aimed specifically at restricting mobility. Improvements in future infectious disease response could be made by incorporating contextually-based mobility factors, for example: the personalization of public health messaging through the recruitment of family members and trusted local leaders, to convey information that would help allay fear and combat stigmatization; activating existing traditional community surveillance systems in which entry into the community must first be approved by the community chief; and increased involvement of local leaders and community members in the provision of food and care to those quarantined so that the need to travel for these reasons is removed.
Prevalence and characteristics of cannabis-induced toxicoses in pets: Results from a survey of veterinarians in North America
Authors: Amissah Richard Quansah , Vogt Nadine A. , Chen Chuyun , Urban Karolina , Khokhar Jibran
Published: April 2022

Cannabis legalization in North America has coincided with an increase in reports of cannabis-induced toxicosis in pets, but the magnitude of this problem, as well as outcomes of these incidents remain unknown. Therefore, we examined the frequency, diagnostic criteria, clinical signs, and prognoses of cannabis toxicoses in pets in North America. We conducted an online survey between January, 2021 and April, 2021 targeting veterinarians practicing in Canada and the United States (US). Out of the 251 study participants, 191 practiced in Canada. Cannabis toxicosis was most commonly reported in dogs (n = 226 veterinarians), and the number of toxicosis cases increased significantly in Canada (p<0.0001) and the US (p = 0.002) after October, 2018. Frequently reported clinical signs of cannabis toxicosis included: urinary incontinence (n = 195), disorientation (n = 182), ataxia (n = 178), lethargy (n = 150), hyperesthesia (n = 134), and bradycardia (n = 112). Edibles were most commonly suspected to be the cause of toxicosis (n = 116). The most common route of exposure was ingestion (n = 135), while the most cited reason was ingestion while unattended (n = 135). Cannabis toxicosis was mostly diagnosed using supportive clinical signs (n = 229), the most common treatment was outpatient monitoring (n = 182), and pets were most often treated as out-patients (n = 103). The legalization of cannabis use in Canada and the US is likely an important factor associated with the increased cannabis toxicosis cases in pets; however, the legal status may also increase reporting. The medicinal use of cannabis by pet-owners for pets may also contribute to a portion of the reported toxicoses. Most pets that experienced cannabis toxicosis recovered completely, suggesting that most cannabis toxicoses do not result in long-term ill effects. Even though some deaths (n = 16) were reported in association with cannabis toxicosis, the presence of confounders such as toxins, and underlying conditions cannot be ruled out, emphasizing the need for rigorous controlled laboratory studies to investigate this important issue.
Mineralized belemnoid cephalic cartilage from the late Triassic Polzberg Konservat-Lagerstätte (Austria)
Authors: Lukeneder Petra , Lukeneder Alexander
Published: April 2022

Although hyaline cartilage is widely distributed in various invertebrate groups such as sabellid polychaetes, molluscs (cephalopods, gastropods) and a chelicerate arthropod group (horseshoe crabs), the enigmatic relationship and distribution of cartilage in taxonomic groups remains to be explained. It can be interpreted as a convergent trait in animal evolution and thus does not seem to be a vertebrate invention. Due to the poor fossil record of cartilaginous structures, occurrences of mineralized fossil cartilages are important for evolutionary biology and paleontology. Although the biochemical composition of recent cephalopod cartilage differs from vertebrate cartilage, histologically the cartilages of these animal groups resemble one another remarkably. In this study we present fossil material from the late Triassic Polzberg Konservat-Lagerstätte near Lunz am See (Lower Austria, Northern Calcareous Alps). A rich Carnian fauna is preserved here, whereby a morphogroup (often associated with belemnoid remains) of black, amorphous appearing fossils still remained undetermined. These multi-elemental, symmetrical fossils show remarkable similarities to recent cartilage. We examined the conspicuous micro- and ultrastructure of these enigmatic fossils by thin-sectioning and Scanning Electron Microscopy (SEM). The geochemical composition analyzed by Microprobe and Energy Dispersive X-ray Spectroscopy (SEM-EDX) revealed carbonization as the taphonomic pathway for this fossil group. Mineralization of soft tissues permits the 3D preservation of otherwise degraded soft tissues such as cartilage. We examined eighty-one specimens from the Polzberg locality and seven specimens from Cave del Predil (formerly Raibl, Julian Alps, Italy). The study included morphological examinations of these multi-elemental fossils and a focus on noticeable structures like grooves and ridges. The detected grooves are interpreted to be muscular attachment areas, and the preserved branched system of canaliculi is comparable to a channel system that is also present in recent coleoid cartilage. The new findings on these long-known enigmatic structures strongly point to the preservation of cephalic cartilage belonging to the belemnoid Phragmoteuthis bisinuata and its homologization to the cephalic cartilage of modern coleoids.
Identifying the changing age distribution of opioid-related mortality with high-frequency data
Authors: Paul Lauren A. , Li Ye , Leece Pamela , Gomes Tara , Bayoumi Ahmed M. , Herring Jeremy , Murray Regan , Brown Patrick
Published: April 2022

Background

Opioid-related mortality continues to rise across North America, and mortality rates have been further exacerbated by the COVID-19 pandemic. This study sought to provide an updated picture of trends of opioid-related mortality for Ontario, Canada between January 2003 and December 2020, in relation to age and sex.

Methods

Using mortality data from the Office of the Chief Coroner for Ontario, we applied Bayesian Poisson regression to model age/sex mortality per 100,000 person-years, including random walks to flexibly capture age and time effects. Models were also used to explore how trends might continue into 2022, considering both pre- and post-COVID-19 courses.

Results

From 2003 to 2020, there were 11,633 opioid-related deaths in Ontario. A shift in the age distribution of mortality was observed, with the greatest mortality rates now among younger individuals. In 2003, mortality rates reached maximums at 5.5 deaths per 100,000 person-years (95% credible interval: 4.0–7.6) for males around age 44 and 2.2 deaths per 100,000 person-years (95% CI: 1.5–3.2) for females around age 51. As of 2020, rates have reached maximums at 67.2 deaths per 100,000 person-years (95% CI: 55.3–81.5) for males around age 35 and 16.8 deaths per 100,000 person-years (95% CI: 12.8–22.0) for females around age 37. Our models estimate that opioid-related mortality among the younger population will continue to grow, and that current conditions could lead to male mortality rates that are more than quadruple those of pre-pandemic estimations.

Conclusions

This analysis may inform a refocusing of public health strategy for reducing rising rates of opioid-related mortality, including effectively reaching both older and younger males, as well as young females, with health and social supports such as treatment and harm reduction measures.
Factors associated with preterm birth among mothers who gave birth at public Hospitals in Sidama regional state, Southeast Ethiopia: Unmatched case-control study
Authors: Fetene Gossa , Tesfaye Tamirat , Negesse Yilkal , Dulla Dubale
Published: April 2022

Background

Preterm birth remains the commonest cause of neonatal mortality, and morbidity representing one of the principal targets of neonatal health care. Ethiopia is one of the countries which shoulder the highest burden of preterm birth. Therefore, this study was aimed to assess factors associated with preterm birth at public hospitals in Sidama regional state.

Methods

Facility-based case-control study was conducted at public hospitals in Sidama regional state, from 1st June to 1st September/2020. In this study, a total of 135 cases and 270 controls have participated. To recruit cases and controls consecutive sampling methods and simple random sampling techniques were used respectively. Data were collected using pretested structured interviewer-administered questionnaire, and checklist via chart review. Data were entered using EpiData version 3.1 and exported to SPSS version 20 for analysis. Independent variables with P-value < 0.25 in the bivariate logistic regression were candidates for multivariable logistic regression analysis. Finally, statistical significance was declared at P-value < 0.05.

Results

The response rate was 100%. Rural resident (AOR = 2.034; 95%CI: 1.242, 3.331), no antenatal care service utilization (AOR = 2.516; 95%CI: 1.406, 4.503), pregnancy-induced hypertension (AOR = 2.870; 95%CI: 1.519, 5.424), chronic medical problem during pregnancy (AOR = 2.507; 95%CI: 1.345, 4.676), urinary tract infections (AOR = 3.023; 95%CI: 1.657, 5.513), birth space less than 2 years (AOR = 3.029; 95%CI: 1.484, 6.179), and physical intimate violence (AOR = 2.876; 95%CI: 1.534, 5.393) were significantly associated with preterm birth.

Conclusion

Most of the risk factors of preterm birth were found to be modifiable. Community mobilization on physical violence during pregnancy and antenatal care follow-up are the ground for the prevention of preterm birth because attentive and critical antenatal care screening practice could early identify risk factors. Besides, information communication education about preterm birth prevention was recommended.
Laboratory and epidemiology data of pertussis cases and close contacts: A 5-year case-based surveillance of pertussis in Indonesia, 2016–2020
Authors: Sunarno Sunarno , Sofiah Sundari Nur , Amalia Novi , Hartoyo Yudi , Rizki Aulia , Puspandari Nelly , Saraswati Ratih Dian , Febriyana Dwi , Febrianti Tati , Susanti Ida , Khariri Khariri , Sariadji Kambang , Muna Fauzul , Rukminiati Yuni , Sulistyaningrum Novi , Riana Dyah Armi , Maha Masri Sembiring , Fitriana Fitriana , Voronika Vivi , Muslih Muamar , Kamal Mushtofa , Setiawaty Vivi
Published: April 2022

Pertussis cases have been reported most frequently in developed countries, but they are predicted to be the most prevalent in developing countries. Indonesia, a developing country, routinely conducts case-based surveillance for pertussis. We reviewed the data on pertussis cases and close contacts based on clinical sample documents examined in the National Reference Laboratory for pertussis, Indonesia (2016–2020). Our objective was to analyze the laboratory and epidemiological aspects of pertussis cases and close contacts, particularly to evaluate the implementation of a 5-year case-based surveillance of pertussis in Indonesia. Data were collected from sample documents and annual laboratory reports between January 2016 and December 2020. We analyzed the proportion of pertussis cases and close contacts by geographic region, year, age, and sex. We used the χ2 test to correlate the laboratory and epidemiological data. In total, 274 clinical cases of pertussis and 491 close contacts were recorded in 15 provinces. The peak number of cases occurred in 2019, with a positivity rate (percentage of laboratory-confirmed cases) of 41.23% (47/114). Clinical cases were dominated by infants aged <1 year (55.5%), and 52.9% of them were aged <6 months. Similarly, 72.3% (68/94) of the laboratory-confirmed cases were infants. Both clinical cases and positivity rates tended to be higher in females (155 cases, 38.1%) than in males (119 cases, 29.4%). No confirmed cases were found in children aged ≥10 years, although positive results still occurred in close contact. Age-group and laboratory-confirmed cases were correlated (p = 0.00). Clinical and confirmed cases of pertussis occurred mostly in the early age group and may be lower in those aged ≥10 years, especially in confirmed cases. New policies are needed for pertussis prevention at an early age, as well as the application of serology tests to increase laboratory-confirmed cases in children aged ≥10 years.
Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish
Authors: Homma Chiho , Inokuchi Daiki , Nakamura Yohei , Uy Wilfredo H. , Ohnishi Kouhei , Yamaguchi Haruo , Adachi Masao
Published: April 2022

The structure of food webs and carbon flow in aquatic ecosystems can be better understood by studying contributing factors such as the diets of herbivorous fish. Metabarcoding using a high-throughput sequencer has recently been used to clarify prey organisms of various fish except herbivorous fish. Since sequences of predator fish have dominated in sequences obtained by metabarcoding, we investigated a method for suppressing the amplification of fish DNA by using a blocking primer or peptide nucleic acid (PNA) clamp to determine the prey organisms of herbivorous fish. We designed three blocking primers and one PNA clamp that anneal to fish-specific sequences and examined how efficient they were in suppressing DNA amplification in various herbivorous fish. The results showed that the PNA clamp completely suppressed fish DNA amplification, and one of the blocking primers suppressed fish DNA amplification but less efficiently than the PNA clamp. Finally, we conducted metabarcoding using mock community samples as templates to determine whether the blocking primer or the PNA clamp was effective in suppressing fish DNA amplification. The results showed that the PNA clamp suppressed 99.3%–99.9% of fish DNA amplification, whereas the blocking primer suppressed 3.3%–32.9%. Therefore, we propose the application of the PNA clamp for clarifying the prey organisms and food preferences of various herbivorous fish.
A workplace Acceptance and Commitment Therapy (ACT) intervention for improving healthcare staff psychological distress: A randomised controlled trial
Authors: Prudenzi Arianna , Graham Christopher D. , Flaxman Paul E. , Wilding Sarah , Day Fiona , O’Connor Daryl B.
Published: April 2022

The levels of psychological distress and burnout among healthcare staff are high, with negative implications for patient care. A growing body of evidence indicates that workplace programmes based on Acceptance and Commitment Therapy (ACT) are effective for improving employees’ general psychological health. However, there is a paucity of research examining the specific psychological and/or behavioural processes through which workplace ACT programmes transmit their beneficial effects. The aim of this randomised controlled trial was to investigate the outcomes and putative processes of change in a 4-session ACT training programme designed to reduce psychological distress among healthcare staff (n = 98). Ninety-eight employees of a healthcare organisation were randomly allocated to the ACT intervention or to a waiting list control group. Study measures were administered on four occasions (baseline, mid-intervention, post-intervention, and follow-up) over a three-month evaluation period. Results showed that the ACT intervention led to a significant decrease in symptoms of psychological distress and a less pronounced reduction in burnout. These effects were mediated primarily via an improvement in mindfulness skills and values-based behaviour and moderated by participants’ initial levels of distress. At four-week post-intervention, 48% of participants who received the ACT intervention showed reliable improvements in psychological distress, with just under half of the aforementioned improvements (46.15%) meeting criteria for clinically significant change. The results advance ACT as an effective stress management intervention for healthcare staff. The findings should be confirmed in a large scale randomised controlled trial with longer follow-up and cost-effectiveness analyses.
Outreach screening to address demographic and economic barriers to diabetic retinopathy care in rural China
Authors: Xiao Baixiang , Mercer Gareth D. , Jin Ling , Lee Han Lin , Chen Tingting , Wang Yanfang , Liu Yuanping , Denniston Alastair K. , Egan Catherine A. , Li Jia , Lu Qing , Xu Ping , Congdon Nathan
Published: April 2022

Importance

Poor access to existing care for diabetic retinopathy (DR) limits effectiveness of proven treatments.

Objectives

We examined whether outreach screening in rural China improves equity of access.

Design, setting and participants

We compared prevalence of female sex, age > = 65 years, primary education or below, and requiring referral care for DR between three cohorts with diabetes examined for DR in neighboring areas of Guangdong, China: passive case detection at secondary-level hospitals (n = 193); persons screened during primary-level DR outreach (n = 182); and individuals with newly- or previously-diagnosed diabetes in a population survey (n = 579). The latter reflected the “ideal” reach of a screening program.

Results

Compared to the population cohort, passive case detection reached fewer women (50·8% vs. 62·3%, p = 0·006), older adults (37·8% vs. 51·3%, p < 0·001), and less-educated persons (39·9% vs. 89·6%, p < 0·001). Outreach screening, compared to passive case detection, improved representation of the elderly (49·5% vs. 37·8%, p = 0·03) and less-educated (70·3% vs. 39·9%, p<0·001). The proportion of women (59.8% vs 62.3%, P>0.300) and persons aged > = 65 years (49.5% vs 51.3%, p = 0.723) in the outreach screening and population cohorts did not differ significantly. Prevalence of requiring referral care for DR was significantly higher in the outreach screening cohort (28·0%) than the population (14·0%) and passive case detection cohorts (7·3%, p<0·001 for both).

Conclusions and relevance

Primary-level outreach screening improves access for the poorly-educated and elderly, and removes gender inequity in access to DR care in this setting, while also identifying more severely-affected patients than case finding in hospital.
The prevalence of alcohol-involved crashes across high and low complexity road environments: Does knowing where drinking drivers crash help explain why they crash?
Authors: Johnson Mark B.
Published: April 2022

Objective

Alcohol use has been linked to impairment of cognitive and psychomotor driving skills, yet the extent to which skill impairment contributes to actual crashes is unknown. A reasonable assumption is that some driving situations have higher skill demands than others. We contend that intersections, the presence of other vehicles or moving objects, and work zones are examples of common situations with higher skill demands. Accordingly, if skill deficits are largely responsible for alcohol-involved crashes, crashes involving a drinking driver (versus only sober drivers) should be overrepresented in these driving situations.

Method

Publicly available FARS data from 2010 to 2017 were collected. Fatal crashes were coded as alcohol-involved (1+ driver with a blood alcohol concentration [BAC] ≥ .05 g/dl) or having no impaired driver (BACs = .000). Drug-positive crashes were excluded. Crashes were also coded as involving moving versus stationary objects, occurring at versus away from intersections, being multivehicle versus single vehicle, occurring at or away from work zones.

Results

Across multiple models, controlling for time of day and type of road, alcohol-involved crashes were significantly underrepresented in crashes at intersections, with moving objects, and other vehicles. Most strikingly, alcohol-involved crashes were 24 percentage points more likely to be with a stationary object than a moving object.

Conclusions

No evidence supported the idea that skill reductions are a primary contributor to alcohol-involved crashes. Alternative explanations and limitations are discussed.
Comparing the hydrological performance of an irrigated native vegetation green roof with a conventional Sedum spp. green roof in New York City
Authors: Shetty Nandan H. , Elliott Robert M. , Wang Mark , Palmer Matthew I. , Culligan Patricia J.
Published: April 2022

The objective of this study was to compare the hydrological performance of an irrigated, 127 mm deep green roof, planted with vegetation native to the New York City area, to a conventional, non-irrigated, 100 mm deep green roof, planted with drought-tolerant Sedum spp. Four years of climate and runoff data from both green roofs were analyzed to determine seasonal stormwater retention. Empirical relationships between rainfall and runoff were developed for both roofs, and applied to historical rainfall data in order to compare stormwater retention values for different rainfall depths. Crop coefficients for the vegetation on each green roof were estimated using the soil moisture extraction function. This function was also used to estimate monthly evapotranspiration. Despite being irrigated, the green roof with native vegetation retained more stormwater per annum (64%) than the non-irrigated green roof planted with Sedum spp. (54%). The green roof planted with native vegetation also had approximately twice the crop coefficient (1.13) than the green roof planted with Sedum spp. (0.57), indicating that the New York City native plants transpire more stormwater than the Sedum spp. plants given certain climate and substrate moisture conditions. Overall, the results of the study indicate that, for the New York City climate region, irrigated green roofs of native vegetation have the capacity to better manage stormwater than non-irrigated green roofs planted with drought-tolerant succulents.
Novel aerosol treatment of airway hyper-reactivity and inflammation in a murine model of asthma with a soluble epoxide hydrolase inhibitor
Authors: Zhang Chuanzhen , Li Wei , Li Xiyuan , Wan Debin , Mack Savannah , Zhang Jingjing , Wagner Karen , Wang Chang , Tan Bowen , Chen Jason , Wu Ching-Wen , Tsuji Kaori , Takeuchi Minoru , Chen Ziping , Hammock Bruce D. , Pinkerton Kent E. , Yang Jun
Published: April 2022

Asthma currently affects more than 339 million people worldwide. In the present preliminary study, we examined the efficacy of a new, inhalable soluble epoxide hydrolase inhibitor (sEHI), 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (TPPU), to attenuate airway inflammation, mucin secretion, and hyper-responsiveness (AHR) in an ovalbumin (OVA)-sensitized murine model. Male BALB/c mice were divided into phosphate-buffered saline (PBS), OVA, and OVA+TPPU (2- or 6-h) exposure groups. On days 0 and 14, the mice were administered PBS or sensitized to OVA in PBS. From days 26–38, seven challenge exposures were performed with 30 min inhalation of filtered air or OVA alone. In the OVA+TPPU groups, a 2- or 6-h TPPU inhalation preceded each 30-min OVA exposure. On day 39, pulmonary function tests (PFTs) were performed, and biological samples were collected. Lung tissues were used to semi-quantitatively evaluate the severity of inflammation and airway constriction and the volume of stored intracellular mucosubstances. Bronchoalveolar lavage (BAL) and blood samples were used to analyze regulatory lipid mediator profiles. Significantly (p < 0.05) attenuated alveolar, bronchiolar, and pleural inflammation; airway resistance and constriction; mucosubstance volume; and inflammatory lipid mediator levels were observed with OVA+TPPU relative to OVA alone. Cumulative findings indicated TPPU inhalation effectively inhibited inflammation, suppressed AHR, and prevented mucosubstance accumulation in the murine asthmatic model. Future studies should determine the pharmacokinetics (i.e., absorption, distribution, metabolism, and excretion) and pharmacodynamics (i.e., concentration/dose responses) of inhaled TPPU to explore its potential as an asthma-preventative or -rescue treatment.
Real-world cost-effectiveness analysis of NOACs versus VKA for stroke prevention in Spain
Authors: Escobar Cervantes Carlos , Martí-Almor Julio , Cabeza Alejandro Isidoro Pérez , Bowrin Kevin , Llorac Moix Aleix , Genís Gironès Mar , Gasche David , Millier Aurélie , Tardu Jean , Toumi Mondher , Briere Jean-Baptiste
Published: April 2022

Aims

A Markov model was adapted to assess the real-world cost-effectiveness of rivaroxaban, dabigatran and apixaban. Each of these non-vitamin K antagonist oral anticoagulants was compared with vitamin K antagonist for stroke prevention in patients with non-valvular atrial fibrillation in Spain.

Methods

All inputs were derived from real-world studies: baseline patient characteristics, clinical event rates, as well as persistence rates for the vitamin K antagonist treatment option. A meta-analysis of real-world studies provided treatment effect and persistence data for rivaroxaban, dabigatran and apixaban, each compared with vitamin K antagonist therapy. The model considered 3-month cycles over a lifetime horizon. The model outcomes included different costs, quality-adjusted life years and life-years gained. Sensitivity analyses were performed to test the robustness of the model.

Results

When compared with vitamin K antagonist, rivaroxaban incurred incremental costs of €77 and resulted in incremental quality-adjusted life years of 0.08. The incremental cost per quality-adjusted life year was €952. For the same comparison, the incremental cost per quality-adjusted life year for dabigatran was €4,612. Finally, compared with vitamin K antagonist, the incremental cost per quality-adjusted life year for apixaban was €32,015. The sensitivity analyses confirmed the robustness of the base case results. The probabilities to be cost-effective versus vitamin K antagonist were 94%, 86% and 35%, respectively, for rivaroxaban, dabigatran and apixaban, considering a willingness-to-pay threshold of €22,000 per quality-adjusted life year gained, based on a cost-effectiveness study of the Spanish National Health System.

Conclusion

These results suggest that rivaroxaban and dabigatran are cost-effective versus vitamin K antagonist for stroke prevention in non-valvular atrial fibrillation, from the Spanish National Health System perspective.