bluuebunny/crossref_metadata_2025 huggingface dataset:
Heparanase Mediates Intestinal Inflammation and Injury in a Mouse Model of Sepsis
Authors: Chen Song , He Ying , Hu Ziwei , Lu Siyu , Yin Xiaohan , Ma Xiaochun , Lv Chuanzhu , Jin Guiyun
Published: February 2017

Heparanase, a heparan sulfate (HS)–specific endoglycosidase, plays an important role in inflammation and mediates acute pulmonary and renal injuries during sepsis. To explore its role in septic intestinal injury, a non-anticoagulant heparanase inhibitor, N-desulfated/re- N-acetylated heparin (NAH), was administrated to a mouse sepsis model induced by cecal ligation and puncture (CLP). Immunohistochemical staining revealed massive shedding of HS from the intestinal mucosal surfaces after CLP, and effective inhibition of heparanase by NAH was confirmed by markedly reduced HS shedding. Following CLP, intestinal expression of heparanase was increased, whereas pretreatment with NAH reduced the sepsis-induced upregulation of heparanase expression. Meanwhile, CLP led to shedding of syndecan-1 and upregulated expression of proteases such as matrix metalloprotease-9 and urokinase-type plasminogen activator in the intestine, whereas NAH markedly suppressed syndecan-1 shedding and protease upregulation following CLP. In addition, pretreatment with NAH attenuated intestinal injury, inhibited neutrophil infiltration and suppressed the production of inflammatory cytokines (tumor necrosis factor–α, interleukin-1β, and interleukin-6) in the intestine during sepsis, and it also significantly reduced the elevation of inflammatory cytokines in the serum 24 hr after CLP. Our findings demonstrate that the activation of intestinal heparanase contributes to intestinal injury during early sepsis by facilitating the destruction of mucosal epithelial glycocalyx and promoting inflammatory responses.
The Assessment of Patient Satisfaction and Attendance of Community Pharmacies in Slovakia
Authors: Mináriková D. , Malovecká I. , Lehocká Ľ. , Snopková M. , Foltán V.
Published: February 2017

Aim: Patient-oriented pharmaceutical care in pharmacies could improve patient satisfaction and influence patient’s choice of a community pharmacy. The aim of the work was to assess patient satisfaction and attendance in community pharmacies in Slovakia. Methods: A self-administered, anonymous questionnaire was distributed to patients visiting 33 community pharmacies in a total of 23 Slovak cities during October-December 2013. The questionnaire contained 29 items and three dimensions were supposed (managing therapy, interpersonal relationship, general satisfaction). A 5-point Likert-type scale (1 = very satisfied, 2 = satisfied, 3 = neither satisfied nor dissatisfied, 4 = dissatisfied, 5 = very dissatisfied) was used for assessing patient satisfaction. The results are presented in percentages and by the level of satisfaction within the range: high satisfaction (score 1.00-2.50), moderate satisfaction (score 2.51-3.50) and low satisfaction (score 3.51-5.00). Reasons for attendance in the community pharmacy and factors influencing pharmacy choice were evaluated either. Results: A total 2 844 respondents were included into the survey. Patient satisfaction with pharmaceutical care achieved high satisfaction level: interpersonal relationship (1.85 ± 0.598; 86.7% highly satisfied respondents) and general satisfaction (2.02 ± 0.643; 71.3% highly satisfied respondents). Managing therapy scored lower (2.24 ± 0.704; 65.4% highly satisfied respondents). The most frequently reported reasons for attending community pharmacy were to obtain prescription (70.4%) and over-the-counter medications (70.4%). The patient choice of a particular pharmacy was influenced by its location (74.1%). Conclusion: This study presents the first nationwide patientreported outcomes about patient satisfaction and attendance in community pharmacies in Slovakia. Future development and advancement of pharmacy practice leading to higher patient satisfaction requires modification of community pharmacists’ professional behaviour in Slovakia namely in managing therapy of patients.
Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel
Authors: Repka Vladimír , Fiala Roderik , Čiamporová Milada , Martinka Michal , Pavlovkin Ján
Published: February 2017

Here, we report the effectiveness of antibody microarray expression profiling (AbMEP) procedure to determine similarities and differences between two maize cultivars (Premia and Blitz) exposed to various concentration of cadmium (Cd), nickel (Ni), as well as simultaneous exposure to both metals (Cd + Ni) for 0, 12 and 24 h. After protein extraction from control (untreated) and heavy metals (HM)-treated root pairs and their fluorescent labelling, the protein extracts were used for the AbMEP procedure and western blot analysis. The results from the microarray were analysed using an internally normalised ratio. Using this highly parallel AbMEP-approach, the timing, dynamics and regulation of the expression of 101 specific genes in untreated and HM-treated roots of maize cultivars were determined. The microarray results revealed 23, 15 and 10 up-regulated/down-regulated proteins in Cd, Ni and Cd + Ni treated cv. Premia roots, respectively. In contrast, the microarray results revealed that 15, 11 and 7 proteins were up-regulated/down-regulated in Cd, Ni and Cd + Ni treated cv. Blitz roots, respectively. The data obtained from the AbMEP array experiment were validated by western blot analyses with more than 89% (the Pearson’s correlation coeficient Rr = 0.78) correlation between the two methods.
Ornements transfrontières/avant-gardes marocaines : entre ésotérisme et post-colonialisme
Authors: Montazami Morad
Published: February 2017

The discourses surrounding the ornament in Western modernist and formalist art theory (Riegl, Focillon) encourage such ideas as the emancipation of the ornament. The same preoccupation has been followed – from a totally different perspective – by Moroccan avant-garde painters (Ahmed Cherkaoui, Farid Belkahia and the Casablanca School) who stood up for the decolonization of arts and culture. How can we retrace the visual and literary genealogy (in practice and theory) that led to this esoteric use of the ornament as a tool for mobility and migration through strategies of sign making and identity politics?
Bayesian environment representation, prediction, and criticality assessment for driver assistance systems
Authors: Schreier Matthias
Published: February 2017

The dissertation approaches the questions i) how to represent the driving environment in an environment model, ii) how to
obtain such a representation, and iii) how to predict the traffic scene for criticality assessment. Bayesian inference
provides the common framework of all designed methods. First, Parametric Free Space (PFS) maps are introduced, which
compactly represent the vehicle environment in form of relevant, drivable free space. They are obtained by a novel method
for grid mapping and tracking in dynamic environments. In addition, a maneuver-based, long-term trajectory prediction and
criticality assessment system is introduced and the application of all methods within the advanced driver assistance
system PRORETA 3 is described.
Physiological responses of Porphyra haitanensis (Rhodophyta) to copper and cadmium exposure
Authors: Zhu Xifeng , Zou Dinghui , Huang Yanhua , Cao Junming , Sun Yuping , Chen Bing , Chen Xiaoying
Published: February 2017

The aim of the present work was to evaluate the effects of copper (Cu) and cadmium (Cd) on the physiology of the red macroalga Porphyra haitanensis Chang and Zheng. The alga was cultured in media with different Cu or Cd concentrations for 7 days. The relative growth rate was significantly reduced at Cu concentrations of 0.10 mg l−1 and above or at Cd concentrations of 4 mg l−1 and above. Chlorophyll a (Chl a), carotenoids (Car), phycoerythrin (PE) and phycocyanin (PC) contents decreased at high Cu or Cd concentrations, and PE content increased at 0.10 mg l−1 Cu or 4–8 mg l−1 Cd. Moreover, the optimal quantum yield, maximum net photosynthetic rate (Pm) and apparent photosynthetic efficiency (α) were significantly reduced at high Cu or Cd concentrations. In contrast, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity increased in response to Cu or Cd treatment. Catalase activity increased at 2–8 mg l−1 Cd and significantly declined at 0.50 mg l−1 Cu or 10 mg l−1 Cd. Nitrate reductase (NR) activity declined at high Cu or Cd concentrations. Consequently, both Cu and Cd were physiological stressors for P. haitanensis, with Cu being more toxic than Cd. This work has generated valuable knowledge about the toxic effects of Cu and Cd on macroalgae.
Bio-based polymer from heterogeneous catalytic polymerization of vegetable oils
Authors: Latifi Seyed Mahdi , Salehirad Alireza , Soltani Atefeh , Bakhshi Azghandi Javad
Published: February 2017

Recently, printing ink ingredients have been provided from vegetable oils as a renewable, non-toxic, and biodegradable resource. In this work, to produce bio-based polymer potentially exploitable in printing ink vehicles, tris(ethylenediamine)nickel(II) nitrate and bis(ethylenediamine)copper(II) nitrate complexes were synthesized, characterized, and then used as heterogeneous catalysts for polymerization of soybean oil. Also, nickel and copper oxide catalysts were fabricated through thermolysis of the mentioned complexes and, after characterization, were employed for the polymerization reaction. The results showed that the presence of nickel and copper complex catalysts favored the reaction progress as the viscosity of the reaction product was increased 4 and 1.3 times, respectively, compared with polymerization without a catalyst. Also, the results obtained from using nickel and copper oxides indicated that these metal oxides do not have a significant effect on the reaction process.
A QoS Control Scheme based on Software Defined Fiber-Wireless Access Network for Survivability
Authors: Ren Danping , Zhang Lijing , Zhao Jijun , Zhao Tianwei , Wu Shanshan
Published: February 2017

Fiber-Wireless (FiWi) access network is a promising broadband access network. Taking its heterogeneous structure into consideration, the paper introduces the Software Defined Networks (SDN) into FiWi access network. Survivability is an important issue in FiWi access network. Once there is a single fiber fails, many high-rate traffic flows may loss. A failure detection scheme is proposed for one distribution fiber fault in the new architecture. For the affected data flows recovery, we focus on the bandwidth allocation scheme for different priorities queues to meet the QoS. The simulation results show that the proposed scheme can maintain the QoS performance in terms of delay, loss rate and network throughput. The survivability is improved efficiently.
Serum proPSA as a marker for reducing repeated prostate biopsy numbers
Authors: Ayyıldız Sema Nur , Noyan Tevfik , Ayyıldız Ali , Benli Erdal , Çırakoğlu Abdullah , Ayyıldız Cemal
Published: February 2017

Introduction

Prostate specific antigen (PSA) has a lower sensitivity and specifity range of 4–10 ng/mL. We aimed to investigate the effectiveness of pPSA in reducing number of prostate biopsies.

Methods

This study enrolled 80 patients aged 50 years or older whom had serum total PSA levels between 4 ng/dL and 10 ng/dL. Age, prostate volume, tPSA, fPSA, pPSA, PSA%, pPSA%, t/pPSA, f/pPSA, p/fPSA, p/tPSA, f/p/tPSA, p/f/tPSA, PSAD, fPSA/PSAD, pPSA/PSAD, (Prostate Health Index) PHI, (t/f/pPSA)/tPSA, and PHI2 (New Prostate Health Index) biopsy results were compared between subjects BPH and PCa.

Results

Out of 80 subjects, 23 (29%) had PCa and 57 (71%) had BPH. Prostate volume was 51.65 mL in PCa and 64.85 mL in non-PCa group (p>0.05). The rate of PCa increased as prostate volume was reduced and age increased. fPSA, PSA%, p/f/tPSA, fPSA/PSAD values were significant in favor of respectively; BPH, BPH, PCa and BPH (p<0.05).

Discussion

Using prostate health index (PHI) was beneficial for predicting PCa. In addition, using pPSA in formulas such as (PHI2) pPSA/(fPSA*√tPSA), p/f/tPSA, (t/f/pPSA)/tPSA may also be useful. This study suggests that the use of pPSA may have a role in reducing the number of prostate biopsies in differentiating PCa and BPH.
NT-proBNP levels in β-thalassemia major patients without cardiac hemosiderosis
Authors: Kurtoğlu Ayşegül Uğur , Karakuş Volkan , Kurtoğlu Erdal , Bozkurt Selen
Published: February 2017

Introduction

Heart failure due to hemosiderosis is frequent in beta-thalassemia major (β-TM) patients. Magnetic resonance imaging (MRI) is used in the early detection of heart failure. Amino-terminal pro-brain natriuretic peptide (NT-proBNP) is a very sensitive marker in the diagnosis of heart failure. In this study, we aimed to investigate the efficacy of NT-proBNP levels in thalassemia patients, who are thought to have no cardiac iron deposition according to T2* scoring system (CMRT2*>20 msn), in early identification of cardiac failure.

Methods

NT-proBNP levels of 31 patients, who have T2*>20 ms, and of 25 healthy population were measured by chemoluminescence method.

Results

NT-proBNP levels were not different in thalassemic patients [median: 33 (IQR: 28–94) pg/mL] compared to control group [median: 41 (IQR: 28–59) pg/mL]. We found that NT-proBNP level was above cut-off value in six patients.

Conclusion

NT-proBNP is a cheaper, reachable, and noninvasive method compared to MRI technique, it can be easily used in monthly controls. Detection of high NT-proBNP levels above cut-off values in patients whose T2* values are normal indicates that measurement of NT-pro-BNP is a more sensitive marker in early detection of cardiac failure.
Ubiquitin Ligase RNF138 Promotes Episodic Ataxia Type 2-Associated Aberrant Degradation of Human Cav2.1 (P/Q-Type) Calcium Channels
Authors: Fu Ssu-Ju , Jeng Chung-Jiuan , Ma Chia-Hao , Peng Yi-Jheng , Lee Chi-Ming , Fang Ya-Ching , Lee Yi-Ching , Tang Sung-Chun , Hu Meng-Chun , Tang Chih-Yung
Published: February 2017

Voltage-gated CaV2.1 channels comprise a pore-forming α1Asubunit with auxiliary α2δ and β subunits. CaV2.1 channels play an essential role in regulating synaptic signaling. Mutations in the human gene encoding the CaV2.1 subunit are associated with the cerebellar disease episodic ataxia type 2 (EA2). Several EA2-causing mutants exhibit impaired protein stability and exert dominant-negative suppression of CaV2.1 wild-type (WT) protein expression via aberrant proteasomal degradation. Here, we set out to delineate the protein degradation mechanism of human CaV2.1 subunit by identifying RNF138, an E3 ubiquitin ligase, as a novel CaV2.1-binding partner. In neurons, RNF138 and CaV2.1 coexist in the same protein complex and display notable subcellular colocalization at presynaptic and postsynaptic regions. Overexpression of RNF138 promotes polyubiquitination and accelerates protein turnover of CaV2.1. Disrupting endogenous RNF138 function with a mutant (RNF138-H36E) or shRNA infection significantly upregulates the CaV2.1 protein level and enhances CaV2.1 protein stability. Disrupting endogenous RNF138 function also effectively rescues the defective protein expression of EA2 mutants, as well as fully reversing EA2 mutant-induced excessive proteasomal degradation of CaV2.1 WT subunits. RNF138-H36E coexpression only partially restores the dominant-negative effect of EA2 mutants on CaV2.1 WT functional expression, which can be attributed to defective membrane trafficking of CaV2.1 WT in the presence of EA2 mutants. We propose that RNF138 plays a critical role in the homeostatic regulation of CaV2.1 protein level and functional expression and that RNF138 serves as the primary E3 ubiquitin ligase promoting EA2-associated aberrant degradation of human CaV2.1 subunits.

SIGNIFICANCE STATEMENTLoss-of-function mutations in the human CaV2.1 subunit are linked to episodic ataxia type 2 (EA2), a dominantly inherited disease characterized by paroxysmal attacks of ataxia and nystagmus. EA2-causing mutants may exert dominant-negative effects on the CaV2.1 wild-type subunit via aberrant proteasomal degradation. The molecular nature of the CaV2.1 ubiquitin–proteasome degradation pathway is currently unknown. The present study reports the first identification of an E3 ubiquitin ligase for CaV2.1, RNF138. CaV2.1 protein stability is dynamically regulated by RNF138 and auxiliary α2δ and β subunits. We provide a proof of concept that protecting the human CaV2.1 subunit from excessive proteasomal degradation with specific interruption of endogenous RNF138 function may partially contribute to the future development of a novel therapeutic strategy for EA2 patients.
Safety of Second-Dose Single-Antigen Varicella Vaccine
Authors: Su John R. , Leroy Zanie , Lewis Paige W. , Haber Penina , Marin Mona , Leung Jessica , Woo Emily Jane , Shimabukuro Tom T.
Published: February 2017

BACKGROUND AND OBJECTIVE:

In 2006, routine 2-dose varicella vaccination for children was recommended to improve control of varicella. We assessed the safety of second-dose varicella vaccination.

METHODS:

We identified second-dose single-antigen varicella vaccine reports in the Vaccine Adverse Event Reporting System during 2006 to 2014 among children aged 4 to 18 years. We analyzed reports by age group (4–6 and 7–18 years), sex, serious or nonserious status, most common adverse events (AEs), and whether other vaccines were administered concomitantly with varicella vaccine. We reviewed serious reports of selected AEs and conducted empirical Bayesian data mining to detect disproportional reporting of AEs.

RESULTS:

We identified 14 641 Vaccine Adverse Event Reporting System reports after second-dose varicella vaccination, with 494 (3%) classified as serious. Among nonserious reports, injection site reactions were most common (48% of children aged 4–6 years, 38% of children aged 7–18 years). The most common AEs among serious reports were pyrexia (31%) for children aged 4 to 6 years and headache (28%) and vomiting (27%) for children aged 7 to 18 years. Serious reports of selected AEs included anaphylaxis (83), meningitis (5), encephalitis (16), cellulitis (52), varicella (6), herpes zoster (6), and deaths (7). One immunosuppressed adolescent was reported with vaccine-strain herpes zoster. Only previously known AEs were reported more frequently after second-dose varicella vaccination compared with other vaccines.

CONCLUSIONS:

We identified no new or unexpected safety concerns for second-dose varicella vaccination. Robust safety monitoring remains an important component of the national varicella vaccination program.
Inventory of the sites of geological interest, in the southwestern of Antioquia Colombia
Authors: Rendón-Rivera Albeiro , Henao-Arroyave Angela Maria , Osorio-Cachaya Juan Guillermo
Published: February 2017

This research sought, the inventory of sites of geological interest, in the southwestern Antioquia region consists of 24 municipalities and a total area of 6513 km2, taking as a scale for analysis 1: 25,000 and used several sources of primary information and secondary. The methodology used was exploratory and was based on: literature review, collection of primary data through virtual and surveys, fieldwork for the surveys, visiting outstanding sites and a workshop in schools, finally ending with integration and analysis of all information collected. As important results, it has an inventory of 168 points of geological interest, which is noted for its natural intrinsic attributes or tourism management plans and associated conservation. Of these, 44 sites stand out for their recognition among population, listed among the first, Cerro Tusa (20.1%) of La Pintada Farallones (12.4%), the Citara Farallones (7.7%), the Paramo del Sol and Cerro Bravo (7.1). Finally, municipalities with a larger number of geological sites of interest were reported Jardín (11.9%), Caicedo (11.3%) and Andes, Jericó y La Pintada (6%), and fewer reports Betania who obtained (0.6%).
Geotechnical variations on a tropical soil produced by municipal solid waste leachate
Authors: Quintero-Ramirez Alejandro , Valencia-Gonzalez Yamile , Lara-Valencia Luis Augusto
Published: February 2017

Solid waste disposal has become a problem for municipal administrations due to the population growth and the limited availability of land for such uses, furthermore, the increase of rainfall for long periods produces a large amount of leachate that requires a proper disposal. This study was carried out to analyze the effect of municipal solid waste leachate in soils, in this way, different tests of physical, chemical, mechanical and mineralogical properties were performed on two samples of a residual soil from the Batolito Antioqueño, located in Rionegro city. For the first sample, geotechnical characterization tests were executed in the natural soil condition. On the other hand, the same tests were implemented in the second sample after go through a leachate process. Were found important variations in the properties of the soil affected by the process of contamination when compared with the properties of the natural soil condition.
Characterization of weathering profiles of the crystal rocks of eastern Bucaramanga and definition of hydrogeological properties
Authors: Colegial-Gutiérrez Juan Diego , Forero-Quintero María Camila , Fuentes-Rueda María Alejandra , Gomez Isidro Sully
Published: February 2017

In the process of improving the knowledge of the hydrogeological potential of weathered zones in crystalline rocks and compounds that can behave as aquifers, this article is performed whose area of study is located east of the metropolitan area of Bucaramanga, Santander Massif consists of two main lithological units; the Neis of Bucaramanga and Santander Group plutonic (diorite, granodiorite-tonalite gray quartz monzonite and monzogranite). These rocks have undergone brittle deformation, physico-chemical weathering mainly influenced by the tropical atmosphere of the area also are jointed and fractured creating conditions that facilitate the flow of water and in turn be optimized weathering processes in the region. He appealed to the identification and location of field weathering profiles made a detailed study of outcrops, presenting a thickness of 90.4 m for the profile of the Gneisic rock weathering and 68.5 m for the profile of granodiorite rock, made up 6 steps weathering of which samples each were taken and carry out macroscopic analysis, petrographic and geochemical using thin films, determining the percentage of porosity, humidity, dry unit weight, X-ray fluorescence, electron microscopy Sweeping and Grading; base characterizing weathering profiles and properties, highlighting the potential hydrogeological found in the Range II: Slightly weathered rock and interval V: Completely weathered rock profile of granodiorite rock and Rock Gneisic profile, the interval III : Moderately weathered rock and the range V: Completely weathered rock, since these stages of weathering, moisture peaks present, regular micro fractures and high porosities.
Expressões do português brasileiro com o verbo cair: uma abordagem semântico-pragmática para o ensino de língua adicional
Authors: Behle Nanashara Fagundes , Ibaños Ana Maria T.
Published: February 2017

This study aims to present a way to help learners of Portuguese as an additional language to understand the meaning of expressions of Brazilian Portuguese, which need the context of the communicative process to have their meaning inferred. For this we adopted a semantic-pragmatic approach, with inferential bias proposed by the philosopher Paul Grice (1957, 1975) in his theories of conversation and of meaning. In this paper, we assume expressions of the verb cair because it is a frequent verb in Brazilian Portuguese everyday language, in expressions with a literal meaning and in idioms, assumed by Fernando (1996) as part of idiomaticity process. As a methodology we use Corpus Linguistics (Sardinha, 2004; O'keeffe; Carter; McCarthy, 2007) so we have access to authentic oral and written texts, using the corpus developed and maintained by Mark Davies and Michael J. Ferreira.
Arbustivas forrajeras y ácidos grasos: estrategias para disminuir la producción de metano entérico en bovinos.
Authors: Cardona-Iglesias Juan Leonardo , Mahecha-Ledesma Liliana , Angulo-Arizala Joaquín
Published: February 2017

El objetivo de este trabajo fue analizar el uso de arbustos forrajeros y ácidos grasos poliinsaturados como estrategia nutricional para mitigar la producción de metano entérico en bovinos. Se hizo especial énfasis en el uso de la Tithonia diversifolia (Hemsl.) A. Gray (botón de oro), como especie con potencial antimetanogénico. La búsqueda de información bibliográ ca para llevar a cabo esta revisión se llevó a cabo durante los meses de junio a septiembre de 2015, mediante términos claves. El metano es un gas de efecto invernadero (GEI), el aumento en su concentración atmosférica, es causada principalmente por emisiones del sector agrícola y la industria, pero se estima también que una proporción es emitida por los rumiantes como producto de la fermentación entérica y anaeróbica de la dieta. Lo anterior causa un problema ambiental y de ine ciencia productiva en los sistemas ganaderos alrededor del mundo. Aunque existe controversia sobre el verdadero aporte de metano por los rumiantes y su incidencia sobre la problemática ambiental, la cantidad de emisiones se deben tratar de mitigar. El presente documento hace énfasis en la búsqueda de estrategias nutricionales, tales como la suplementación con especies arbustivas forrajeras y fuentes de ácidos grasos poliinsaturados, los cuales han demostrado tener potencial para mantener la e ciencia productiva del animal y una posible reducción en la síntesis de metano entérico.
Efecto de raza y niveles de energía en la nalización de novillos en pastoreo.
Authors: Ramírez-Barboza José Ignacio , Valverde-Abarca Anthony , Rojas-Bourillón Augusto
Published: February 2017

El objetivo del presente trabajo fue evaluar el efecto del componente racial y la suplementación energética en novillos en etapa de nalización en pastoreo sobre la ganancia de peso, la calidad de la canal y la fuerza de corte (kg). El periodo de evaluación del estudio fue de 144 días, durante el año 2014. Este se realizó en la nca “La Vega” ubicada en el cantón de San Carlos, Alajuela, Costa Rica. Se utilizaron veintiséis animales distribuidos en dos grupos raciales y tres niveles de suplementación para un total de seis tratamientos experimentales: E-T0 (3,2 Mcal), C-T0 (3,2 Mcal), E-T1 (3,4 Mcal), C-T1 (3,4 Mcal), E-T2 (3,67 Mcal) y C-T2 (3,67 Mcal). Se evaluaron: ganancia diaria de peso (GDP) y peso vivo y de la canal, marmoleo, desarrollo muscular, grasa dorsal, rendimiento en canal, espesor de grasa dorsal (EGD), peso de grasa perirenal, área del ojo del lomo (AOL) y nalmente la fuerza de corte (FC) como parámetro de calidad de carne. No se encontraron diferencias debido al efecto de la ración (P>0,05) para ganancia diaria de peso, peso de la canal, marmoleo, desarrollo muscular y grasa dorsal. Se encontró un efecto del componente racial sobre la calidad de carne (P<0,05), en donde los animales europeos presentaron carnes con mayor suavidad, en un periodo de maduración de catorce días (5,32 vs 8,53 kg).
Three new species of Pleurothallis (Orchidaceae: Pleurothallidinae) in subsection Macrophyllae-Fasciculatae from northern South America
Authors: Wilson Mark , Baquero Luis , Dupree Katharine , Jiménez Marco M. , LeBlanc Cheryl M. , Merino Gilberto , Portilla Jose , Salas Guerrero Marcos , Tobar Suárez Francisco , Werner Jon D.
Published: February 2017

The history of the taxonomy of Pleurothallis R.Br. subsection Macrophyllae-Fasciculatae and recent descriptions in that group are summarized. The phylogenetic position of the group based on preliminary molecular data and the appropriateness of the proposed genera Acronia C.Presl. and Zosterophyllanthos Szlach. & Marg. for this group are discussed. Three new species from northern South America are described: Pleurothallis rubrifolia from southeastern Ecuador and northeastern Peru; Pleurothallis nangaritzae from southeastern Ecuador; and Pleurothallis castanea. Labellar micromorphology examined by scanning electron microscopy for P. rubrifolia and P. nangaritzae is discussed in relation to taxonomy and possible pollinator interactions.
A TP53-mutáció-analízis jelentősége krónikus lymphocytás leukaemiában
Authors: Fésüs Viktória , Marosvári Dóra , Kajtár Béla , Király Péter Attila , Demeter Judit , Gurbity Pálfi Tímea , Egyed Miklós , Plander Márk , Farkas Péter , Mátrai Zoltán , Matolcsy András , Bödör Csaba
Published: February 2017

Abstract:

Introduction: In recent years much progress has been made in the therapy of chronic lymphocytic leukaemia, as the new innovative medicine proved to be effective in managing patients carrying TP53 abnormalities. To identify all these patients, it is essential to screen for both forms of TP53 defects, including both 17p deletions and TP53 mutations. Aim: The aim of this study was to determine the frequency of TP53 mutations and their association with 17p deletions in a large Hungarian cohort of 196 patients suffering from chronic lymphocytic leukaemia. Method: We performed mutation analysis of TP53 (exons 3–10) using Sanger sequencing. Results: TP53 mutations were present in 15.8% of patients, half of which were associated with 17p deletion. By analysing both forms, TP53 defect was identified in 25.4% of the patients. Conclusions: Our study demonstrates that by performing a TP53 mutation analysis, an additional 10% of high-risk patients can be detected. Orv. Hetil., 2017, 158(6), 220–228.
PERIKANAN BUNBUN (BRUSH PARK FISHERIES) DI SITU BAGENDIT KABUPATEN GARUT PROPINSI JAWA BARAT
Authors: Nurfiarini Amula , Purnomo Kunto
Published: February 2017

Situ Bagendit, Kabupaten Garut Propinsi Jawa Barat mempunyai luas 20-57 ha dan berfungsi utama sebagai obyek wisata ziarah, meskipun aktivitas lainnya juga berkembang cukup pesat, yakniperikanan tangkap. Bunbun merupakan salah satu jenis alat tangkap yang menyerupai rumpon, berbahan ranting bambu dengan luas 15-30m2. Bunbun dioperasikan dengan cara menenggelamkan rumpon yang telah diberi tiang penahan ke perairan pada kedalaman tertentu, dan membiarkannya selama suatu periode tertentu. Pemanenan ikan dilakukan dengan menggunakan bantuan perangkap bambu (wide). Produksi tangkapan bunbun berkisar antara 40-60 kg/unit/musim dengan jenis dan ukuran ikan sangat beragam. Hasil analisis usaha menunjukkan bahwa untuk setiap 5 unit bunbun yang dioperasikan dengan periode penenggelaman 3 bulan akan menghasilkan nilai produksi sebesar Rp 2.192.250, dan keuntungan bersih Rp.558.775 per musim dengan nilai B/C ratio sebesar 2,03. Artinya, bahwa usaha penangkapan dengan bunbun cukup layak diusahakan. Namun demikian perlu diwaspadai bahwa perkembangan bunbun yang pesat tanpa diiringi aturan mengenai jumlah optimal yang diperkenankan, lokasi peletakkan, dan sistem operasional bunbun termasukpemeliharaannya, keberadaan bunbun dapat mengurangi nilai estetika situ, bahkan dapat mempercepat terjadinya pendangkalan.
KARAKTERISTIK FISIKA KIMIA PERAIRAN DAN STRUKTUR KOMUNITAS ZOOPLANKTON DI ESTUARI SUNGAI BANYUASIN, SUMATERA SELATAN
Authors: Prianto Eko , Husnah Husnah , Aprianti Solekha
Published: February 2017

Penelitian ini bertujuan untuk mengetahui kondisi kualitas perairan pasca pembangunan Pelabuhan Tanjung Api-Api dengan mempelajari karakteristik fisika kimia perairan dan struktur komunitas zooplankton. Penelitian tentang kelimpahan dan keanekaragaman jenis zooplankton dilakukan melalui enam titik stasiun pengamatan di muara Sungai Banyuasin pada bulan April sampai Nopember 2009. Contoh air diambil pada kedalaman 1 m dari permukaan air dengan menggunakan kemmerer water sampler dan contoh zooplankton diambil dengan menggunakan ember 50 L pada permukaan perairan. Selanjutnya contoh air disaring dengan menggunakan planktonnet dengan ukuran 25 μm. Hasil analisis kualitas air diperoleh nilai parameter (suhu, pH, NO2, COD, BOD, dan DO) berada dalam ambang batas yang diperbolehkan sesuai dengan Peraturan Pemerintah No.82 Tahun 2001. Nilai parameter padatan tersuspensi (total suspended solids), total dissolvedsolid, amoniak, nitrat, dan fosfat telah melebihi ambang batas lingkungan perairan yang diperbolehkan. Nilai indeks mutu lingkungan perairan menunjukan dalam kondisi tercemar berat. Kelimpahan zooplankton pada tahun 2009 yaitu 2-19 ind/L lebih rendah dari kelimpahan pada tahun 2004 yaitu 5- 34 ind/L sedangkan keanekaragaman jenis pada tahun 2009 mengalami penurunan jika dibandingkan tahun 2004. The aims of this study were to determine water quality conditions after construction of the port of Tanjung Api-Api by studying the physico chemical characteristics of water and zooplankton community structure. Research on zooplankton abundance and species diversity through the six points of observation stations in the estuary of the river Banyuasin in April until November 2009. Water samples taken at a depth of 1 m from water surface water by using a kemmerer water sampler and zooplankton samples were taken by using a bucket about 50 L in the surface. Further water samples were filtered using a plankton net with a size 25 μm. The results of water quality analize obtained that parameter values (temperature, pH, NO2, COD, BOD, and DO) were still in the threshold limit, in accordance with Government Regulation No.82 2001. Value parameters of total suspended solids, total dissolved solid, ammonia, nitrate, and phosphate has exceeded the permissible limit aquatic environment. Value of water environmental quality index showed in heavily polluted conditions. Zooplankton abundance in was 2-19 ind/L lower than the abundance in 2004 of 5-34 ind/L, while species diversity in the year 2009 decreased compared to 2004.
SEBARAN UKURAN HASIL TANGKAPAN DAN ASPEK REPRODUKSI IKAN SIDAT (Anguilla bicolor bicolor McClelland, 1844) DI PERAIRAN SEGARA ANAKAN, CILACAP
Authors: Herianti Isnani , Nugroho Duto
Published: February 2017

Penelitian biologi sumber daya ikan sidat telah dilakukan di perairan Segara Anakan, Cilacap dengan penekanan pada karakteristik biologi berdasarkan atas sebaran ukuran hasil tangkapan dan keragaan aktivitas reproduksi dalam populasi tersebut. Penampilan reproduksi dianalisis melalui pembuatan penampang lintang gonad dengan metode parafin dan pewarnaan hematoxylin ehrlicheosin terhadap contoh yang dianggap mewakili kelompok ukuran panjang. Hasil penelitian ini menunjukan bahwa sebaran frekuensi panjang mempunyai tipe bimodal dengan kecenderungan perbedaan kisaran ukuran berdasarkan atas musim. Rata-rata ukuran panjang hasil tangkapan berdasarkan atas musim menunjukan beda yang sangat nyata (P<0,01). Keragaan aktivitas reproduksi sebagai bagian dari proses penentuan status pemanfaatan memperlihatkan kisaran pada tingkat belum teridentifikasi, belum berkembang sampai tingkat menuju matang gonad. The biological investigation of eel resources have been conducted in Segara Anakan waters,Cilacap District. This study describes the size distribution and variation of its reproductive activity. The reproductive performances were analyzed through cross section of gonad using paraffin method and stained with hematoxylin ehrlich-eosin to the sample that are considered to represent group of length. The result showed that the length frequency are the typically bimodal distribution with tendencies of differences in size based on seasons. The seasonly average length showed a significantly different (P<0.01). Variation in reproductive activity as part of biological indicator of exploitation level showed some differences level of gonad development stages, starting from unidentified, undevelop, until toward mature gonadal levels.
KUALITAS AIR BAGI KEHIDUPAN ORGANISME BAGIAN TENGAH DAN HILIR SUNGAI MUSI BERDASARKAN PADA SUMBER POLUTAN
Authors: Kaban Siswanta , Husnah Husnah , Aida Siti Nurul
Published: February 2017

Penelitian ini dilakukan untuk mengetahui kualitas air Sungai Musi tahun 2007 sampai dengan 2008 di bagian tengah dan hilir berdasarkan pada sumber polutan. Empat belas stasiun pengambilan contoh ditetapkan sebagai sumber polutan seperti industri maupun pemukiman penduduk, dan referensi yang jauh dari industri maupun pemukiman yang digunakan sebagai pembanding. Pada setiap stasiun, pengambilan contoh dilakukan 3 kali waktu pengambilan, yaitu bulan April, Juni, dan Januari yang dapat mewakili 3 musim yang berbeda pada tahun tersebut. Beberapa parameter diukur in situ sementara beberapa lain dianalisis di laboratorium dengan standar methods (AWWAWEF, 2005). Dari hasil penelitian didapatkan bahwa industri yang bergerak di bidang pengolahan kelapa sawit dan karet cenderung menurunkan kualitas perairan di Sungai Musi. Kandungan logam berat dalam sedimen di Sungai Musi relatif rendah dengan kandungan Cr+6 dan Pb yang tertinggi masing-masing 13,481 dan 1,747 μg per g. Curah hujan cenderung menurunkan beberapa parameter fisika dan kimia kualitas perairan. Potensi pencemaran cenderung ditemukan di bagian hilir Sungai Musi, karena sebaran industri dan intensitas pemanfaatan perairan cukup tinggi di bagian sungai tersebut. Study in order to know distribution of pollution source and its effect on water quality of the middle and down stream of Musi River was conducted in April and June 2007 and January 2008. Fourteen sampling sites were selected based on the pollution source and the minimal degradation site (reference sites). Parameters observed were pollution source distribution and water and sediment parameters such as physical and chemical parameters. Water sample was collected at 0.5 m from water surface by using Kemmerer water sampler while sediment samples were taken by using Ekman grab. Some of the parameters were analyzed in situ while the rest were analyzed in laboratory. Results indicated that oil palm and rubber industries were mostly the pollution source in Musi River. Potential pollution source was mostly found in the middle and down stream of Musi River since most of pollution source and high water utilization found in this area. Water quality parameters except total suspended solid and biochemical oxygen demand, were still in the range that can be tolerated by the aquatic organisms. Rain fall tends to decrease water quality of the river. Concentration of heavy metal such as Chrom (Cr+6) and plumbum in the sediment were in still in low concentration with the highest concentration reaching 13.481 and 1.747 μg per g respectively.
Lagrangian Formulation for Energy-efficient Warehouse Design
Authors: Derpich Ivan , Sepulveda Juan
Published: February 2017

Energy consumption in modern warehouses is today an important issue which has not received much attention in the scientific community. In this paper it is addressed the problem of warehouse design considering the energy costs incurred by vehicles and equipment in a fully or partially automated facility. Closed-form solutions are obtained by a formulating the Lagrangian of an operational cost function with equality constraints. The contribution of the paper is to develop formulas for reduced energy consumption and pollution, both relevant aspects in sustainable engineering systems. An example applied to a distributor of MRO items is presented. In this version the energy cost is integrated into the formula, modifying the method presented in [1].
Pathologic Complete Response with Neoadjuvant Doxorubicin and Cyclophosphamide Followed by Paclitaxel with Trastuzumab and Pertuzumab in Patients with HER2-Positive Early Stage Breast Cancer: A Single Center Experience
Authors: Singh Jasmeet C. , Mamtani Anita , Barrio Andrea , Morrow Monica , Sugarman Steven , Jones Lee W. , Yu Anthony F. , Argolo Daniel , Smyth Lilian M. , Modi Shanu , Schweber Sarah , Boafo Camilla , Patil Sujata , Norton Larry , Baselga Jose , Hudis Clifford A. , Dang Chau
Published: February 2017

Objectives

Trastuzumab (H) and pertuzumab (P) with standard chemotherapy is approved for use in the neoadjuvant setting for human epidermal growth receptor 2 -positive patients. A retrospective analysis was performed of patients treated with dose-dense (dd) doxorubicin and cyclophosphamide (AC) followed by paclitaxel (T), trastuzumab, and pertuzumab (THP) in the neoadjuvant setting. Here, the pathologic complete response (pCR) rates are reported.

Methods

An electronic medical record review was conducted of patients treated with HP-based therapy in the neoadjuvant setting from September 1, 2013, to March 1, 2015. Data on patient demographics, stage of breast cancer, pathology reports, surgical data, and information on systemic therapy were collected. The pCR was defined as total (tpCR, ypT0/is ypN0), German Breast Group (GBG) pCR (ypT0 ypN0), breast pCR (bpCR) with in situ disease (ypT0/is) and without in situ disease (ypT0), and explored axillary pCR (ypN0).

Results

Charts from 66 patients were reviewed, and 57 patients were evaluable for pCR. Median age was 46 years (range 26–68 years). Median tumor size was 4 cm. Of 57 patients, 53 (93%) had operable breast cancer (T1-3, N0-1, M0). Three patients (5.3%) had locally advanced disease (T2-3, N2-3, M0 or T4a-c, any N, M0), and 1 (1.7%) had inflammatory breast cancer (T4d, any N, M0). Overall, 44 (77%) and 13 (23%) had hormone receptor (HR)-positive and negative diseases, respectively. Median numbers of cycles of neoadjuvant treatment were as follows: AC (4, range 1–4), T (4, range 1–4), trastuzumab (6, range 3–8), and pertuzumab (6, range 2–8). In these 57 patients, the rates of tpCR and bpCR with in situ disease were demonstrated in 41/57 (72%) patients, and the rates of GBG pCR and bpCR without in situ disease were found in 30/57 (53%) patients. Of 26 patients with biopsy-proven lymph nodal involvement, axillary pCR occurred in 22 (85%) patients.

Conclusion

At a single center, the tpCR and GBG pCR rates of dd AC followed by THP are high at 72% and 53%, respectively.
Financial Toxicity and Societal Costs of Cancer Care: Distinct Problems Require Distinct Solutions
Authors: Peppercorn Jeffrey
Published: February 2017

Control of societal costs can be addressed through clinician education, value-based guidelines and pathways, formulary restrictions, payment reform, and government regulatory decisions but should not be imposed on patients at the point of decision-making in the cancer clinic.
The Anatomy of Financial Vulnerabilities and Crises
Authors: Lee Seung Jung , Posenau Kelly E. , Stebunovs Viktors
Published: February 2017

We extend the framework used in Aikman, Kiley, Lee, Palumbo, and Warusawitharana (2015) that maps vulnerabilities in the U.S. financial system to a broader set of advanced and emerging economies. Our extension tracks a broader set of vulnerabilities and, therefore, captures signs of different types of crises. The typical anatomy of the evolution of vulnerabilities before and after a financial crisis is as follows. Pressures in asset valuations materialize, and a build-up of imbalances in the external, financial, and nonfinancial sectors follows. A financial crisis is typically followed by a build-up of sovereign debt imbalances as the government tries to deal with the consequences of the crisis. Our early warnings indicators which aggregate these vulnerabilities predict banking crises better than the Credit-to-GDP gap at long horizons. Our indicators also predict the severity of banking crises and the duration of recessions, as they take into account possible spill-over and amplification channels of financial stress to from one to another sector in the economy. Our indicators are of relevance for macroprudential and crisis management, in part, because they perform better than the Credit-to-GDP gap and do not suffer from the gaps econometric flaws.
Fatores de Risco para Infecção Pós-Craniotomia
Authors: Irffi Gustavo Palmer , Tofani Gabriel Bandeira , Da Silva Cynthia Mendes , Vieira Felipe Coelho , Da Silva Isabela Lorena Alfenas , Couto Bráulio Roberto Gonçalves Marinho , Miranda Gilberto Diniz , Starling Carlos Ernesto Ferreira
Published: February 2017

Justificativa e Objetivos: A infecção pós-craniotomia é um risco real para a recuperação do paciente, com aumento da morbimortalidade e, também, dos custos para o sistema de saúde. Com uma alta incidência de complicações, chegando a até 11%, é importante ter noção dos fatores de risco desse procedimento a fim de melhorar a qualidade do atendimento e da recuperação do paciente. Dessa forma, o objetivo desse artigo é definir o risco de infecção nesse procedimento; indicar a incidência de infecção de sítio cirúrgico e de meningite; apontar os principais fatores de risco; e calcular a taxa de óbito de craniotomia. Métodos: O estudo foi uma coorte retrospectiva em seis hospitais de Belo Horizonte por um período de dez anos. Dados foram colhidos e analisados buscando resultados relacionados à incidência e aos fatores de risco pós-craniotomia. Resultados: As infecções globais têm uma incidência de 8,8%, as infecções de sítio cirúrgico de 5,1% e as meningites de 2,3%. A taxa de letalidade está em 8,3%. Conclusão: Os principais fatores de risco são o escore American Society of Anesthesiologists (ASA) > 2 e o uso de próteses; o uso de anestesia geral se mostrou um fator protetor em relação ao desenvolvimento de infecções.
Avaliação de Ibicella lutea como agente antimicrobiano frente à Staphylococcus aureus
Authors: Volcão Lisiane Martins , Silveira Coelho Tatiane , Vàsquez Alváro , Pia Cerdeiras Maria , Almeida da Silva Pedro Eduardo , Rodrigues da Silva Júnior Flávio Manoel , Fernandes Ramos Daniela
Published: February 2017

Justificativa e Objetivos: O estudo objetivou a avaliação da atividade antibacteriana de extratos brutos e frações de Ibicella lutea, na inibição do crescimento de Staphylococcus aureus, avaliando a combinação destes compostos e sua atividade citotóxica. Métodos: Para a atividade antibacteriana dos extratos foi utilizado o Teste de Microdiluição em Caldo, seguido do Teste Checkerboard. Os extratos que apresentaram atividade antibacteriana foram submetidos ao teste de citotoxicidade, com células macrofágicas e determinação do seu Índice de Seletividade (IS). Resultados: A fração de acetato de etila(AcOE) obteve o melhor potencial antibacteriano (6,25 µg/mL), entretanto nenhum dos compostos testados apresentaram atividade bactericida nas concentrações empregadas. Neste estudo pode-se observar uma ação aditiva entre as frações AcOE e metanólica (MeOH), sendo a interação entre os extratos brutos indiferente. De acordo com o teste de citotoxicidade, a fração AcOE apresentou uma maior índice de sobrevivência das células macrofágicas (IC50%=30,35 µg/mL). Entretanto, quando calculado o IS, não houve resultados satisfatórios (IS < 10) para os extratos analisados. Conclusões: Neste estudo observou-se o potencial antibacteriano das frações AcOE e MeOH, assim como uma ação aditiva na combinação das frações, dando suporte para o isolamento e caracterização de seus componentes ativos. Apesar dos extratos não apresentarem um IS satisfatório, novos estudos de toxicidade devem ser realizados para determinar com segurança o potencial de uso dos produtos provenientes de I. lutea, como é o caso de medicamentos para uso tópico ou biocidas.
“If doubt it ye cannot, then play unto its riddle the oedipus”: phillhelenic patterns in Edgar Allan Poe's tales
Authors: Tsokanos Dimitrios
Published: February 2017

Edgar Allan Poe's bicentenary triggered the interest of multiple scholars and academics and a number of researches based on Poe's works and their impact outside the United States have been produced. Quite astonishingly, even in Lois Vine's Poe Abroad (1999), a meticulous investigation upon the latter matter, Greece is nowhere to be mentioned. Apart from scarce mentions on Poe's influence by and on the Greek realm so far, the presence of Greek motifs in the American author's works has merely been delved into up to date. This paper aims to analyze this particular topic through Poe's Eleonora and Ligeia and it will attempt to demonstrate the philhellenic patterns existing in the literary idol's masterpieces along with the reasons why he turned to Greece as a source of inspiration.