bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurophotonic methods in approach to in vivo animal epileptic models: Advantages and limitations
Authors: Tsytsarev Vassiliy , Sopova Julia V. , Leonova Elena I. , Inyushin Mikhail , Markina Alisa A. , Chirinskaite Angelina V. , Volnova Anna B.
Published: December 2023

Neurophotonic technology is a rapidly growing group of techniques that are based on the interactions of light with natural or genetically modified cells of the neural system. New optical technologies make it possible to considerably extend the tools of neurophysiological research, from the visualization of functional activity changes to control of brain tissue excitability. This opens new perspectives for studying the mechanisms underlying the development of human neurological diseases. Epilepsy is one of the most common brain disorders; it is characterized by recurrent seizures and affects >1% of the world's population. However, how seizures occur, spread, and terminate in a healthy brain is still unclear. Therefore, it is extremely important to develop appropriate models to accurately explore the causal relationship of epileptic activity. The use of neurophotonic technologies in epilepsy research falls into two broad categories: the visualization of neural epileptic activity, and the direct optical influence on neurons to induce or suppress epileptic activity. An optogenetic variant of the classical kindling model of epileptic seizures, in which activatable cells are genetically defined, is called optokindling. Research is also underway concerning the application of neurophotonic techniques for suppressing epileptic activity, aiming to bring these methods into clinical practice. This review aims to systematize and describe new approaches that use combinations of different neurophotonic methods to work with in vivo models of epilepsy. These approaches overcome many of the shortcomings associated with classical animal models of epilepsy and thus increase the effectiveness of developing new diagnostic methods and antiepileptic therapy.
Association between dynapenia and cognitive decline in community‐dwelling older Japanese adults: The IRIDE Cohort Study
Authors: Hatanaka Sho , Sasai Hiroyuki , Shida Takashi , Osuka Yosuke , Kojima Narumi , Ohta Takahisa , Abe Takumi , Yamashita Mari , Obuchi Shuichi P , Ishizaki Tatsuro , Fujiwara Yoshinori , Awata Shuichi , Toba Kenji ,   IRIDE Cohort Study investigators
Published: December 2023

Aim

Muscle mass and strength correlate with cognitive function; however, it remains unclear whether dynapenia (i.e., muscle weakness with preserved muscle mass) is relevant. This study aimed to explore whether dynapenia is associated with global cognitive function in community‐dwelling older Japanese adults.

Methods

This cross‐sectional study used data from the Integrated Research Initiative for Living Well with Dementia Cohort Study, which pooled data from five community‐based geriatric cohorts. Dynapenia was defined as muscle weakness without muscle mass loss according to the Asian Working Group for Sarcopenia criteria. Cognitive function was assessed using the Mini‐Mental State Examination (MMSE). An ordered logistic regression analysis was conducted with dynapenia as the exposure and with cognitive decline stages, defined as an MMSE score of 27–30 for normal cognition, 24–26 for possible cognitive decline, and <24 for cognitive decline, as the outcome, stratified by sex and adjusted for age, muscle mass, education, alcohol consumption, smoking habits, living alone, and non‐communicable diseases.

Results

We analyzed data for 3338 participants (2162 female) with preserved muscle mass. Of these, 449 (13.5%) had dynapenia, and 79 (2.4%) exhibited cognitive decline. Multivariate odds ratios (95% confidence interval) for cognitive decline among those with dynapenia, compared with those without dynapenia, were 1.51 (1.02–2.24) for males and 2.08 (1.51–2.86) for females.

Conclusions

Muscle weakness is associated with cognitive decline, even in individuals with preserved muscle mass. Further studies are needed to better understand the association between muscle weakness and cognitive decline over time in order to develop dementia prevention strategies for those with dynapenia. Geriatr Gerontol Int 2024; 24: 123–129.
Algorithmic approach toward diagnosis of patients with congenital photosensitivity disorders and review of literature
Authors: Patro Nibedita , Sathishkumar Dharshini , Panda Maitreyee , Mahajan Rahul ,   Members of SIG Pediatric Dermatology and IADVL Academy
Published: December 2023

The congenital photosensitivity disorders present as cutaneous signs and symptoms secondary to photosensitivity, extracutaneous manifestations, and a predisposition to malignancy. Diagnosis of these conditions mainly depend on clinical findings as the molecular analysis is not always feasible. A review of all the related articles collected after a thorough literature search using keywords, “congenital AND photosensitivity NOT acquired” and the individual diseases was done. A total of 264 articles were included in the review. An algorithm for diagnosis of the different congenital photosensitivity disorders based on the various clinical presentations has been proposed. An early suspicion and diagnosis of the different congenital photosensitivity disorders is the cornerstone behind prompt institution of prevention and treatment, and decreasing the associated morbidity.
The Ontology and Aesthetics of Genre
Authors: Malone Evan
Published: December 2023

Genres inform our appreciative practices. What it takes for a work to be a good work of comedy is different than what it takes for a work to be a good work of horror, and a failure to recognize this will lead to a failure to appreciate comedies or works of horror particularly well. Likewise, it is not uncommon to hear people say that a film or novel is a good work, but not a good work of x (where x is the genre of that work). A work can be good all things considered, but genre membership provides us with an additional set of evaluative criteria over and above those of the medium, which colors how we interpret and appreciate the work. Given this importance, it is not surprising that philosophers of art have been interested in providing an account of what, exactly, a genre is. Despite this interest, there is not widespread agreement about what it takes for something to be a genre, nor what kinds of considerations are relevant in determining whether a work is a member of that genre. Beyond this, we might also want to know to what degree we ought to consider genre in evaluating a work of art and why it should matter at all. Here, I explore the variety of recent theories that philosophers have taken up on the topic of genre and why we should ultimately think of genres as artistic practices rather than the alternatives.
A reconsideration of group differences in social psychology: Towards a critical intersectional approach
Authors: Sabik Natalie J. , Versey H. Shellae
Published: December 2023

Social psychology has focused on patterns of inequality (e.g., discrimination, stereotyping, stigma, intergroup relations) that underlie well‐documented disparities, often without engaging with the structural and intersectional patterns underlying these experiences. In this paper, we draw on intersectionality theory and research to illustrate how approaches to studying inequity and disparities in social psychology reflect a Western perspective, and structures work to uphold the status quo. It is argued that structures within the research process need to be made visible, both in terms of how research questions are framed, as well as on the representation within institutions responsible for producing and disseminating work addressing disparities. Engaging with intersectionality theory and research, we suggest four strategies that address individual and structural approaches to better position social psychology to contribute meaningfully to the reduction of disparate outcomes affecting minoritized individuals and groups. We suggest (a) examining and addressing the sources of inequality (e.g., addressing racism rather than the effects of racism), (b) carefully attending to language and framing in both the causes and impact of inequality and discrimination to shift the status quo in social psychology research and in society, (c) addressing representation within social psychology research, namely in critically examining representation and assumptions made about research samples, and (d) addressing representation in the “structure” of institutions. We offer these approaches, informed by intersectional theory, as tangible strategies to address some of the structural issues underlying inequality and disparities that can be addressed within social psychology.
The Electrification of Railways: An Imperative Need for the Selection of a System for Universal Use
Authors: Westinghouse George
Published: December 2023

As an illustration of the wonders of the laws of nature, few inventions or discoveries with which we are familiar can excel the static transformer of the electrical energy of alternating currents of high voltage into the equivalent energy at a lower voltage.

To have discovered how to make an inert mass of metal capable of transforming alternating currents of 100,000 volts into currents of any required lower voltage with a loss of only a trifle of the energy so transformed, would have been to achieve enduring fame. The facts divide this honor among a few, the beneficiaries will be tens of millions.
Automatic Ground-Truth Image Labeling for Deep Neural Network Training and Evaluation Using Industrial Robotics and Motion Capture
Authors: Helmich Harrison F. , Doherty Charles J. , Costello Donald H. , Kutzer Michael D. M.
Published: December 2023

The United States Navy (USN) intends to increase the amount of uncrewed aircraft in a carrier air wing. To support this increase, carrier-based uncrewed aircraft will be required to have some level of autonomy as there will be situations where a human cannot be in/on the loop. However, there is no existing and approved method to certify autonomy within Naval Aviation. In support of generating certification evidence for autonomy, the United States Naval Academy (USNA) has created a training and evaluation system (TES) to provide quantifiable metrics for feedback performance in autonomous systems. The preliminary use case for this work focuses on autonomous aerial refueling. Prior demonstrations of autonomous aerial refueling have leveraged a deep neural network (DNN) for processing visual feedback to approximate the relative position of an aerial refueling drogue. The training and evaluation system proposed in this work simulates the relative motion between the aerial refueling drogue and feedback camera system using industrial robotics. Ground-truth measurements of the pose between the camera and drogue are measured using a commercial motion capture system. Preliminary results demonstrate calibration methods providing ground-truth measurements with millimeter precision. Leveraging this calibration, the proposed system is capable of providing large-scale datasets for DNN training and evaluation against a precise ground truth.
Robotics and AI in the Global South
Authors: Matsiko Amos , Nelson Bradley J.
Published: December 2023

An increasing presence of robotics and artificial intelligence in the Global South calls for responsible implementation.
Would life be better as a robot?
Authors: Murphy Robin R.
Published: December 2023

A collection of short stories from Africa and the diaspora explore transhumanism in the face of ecological collapse.
Draft genome sequences of eight bacterial isolates from
Pisum sativum
leaf surfaces
Authors: Smee Melanie R. , Herr Kathryn L. , Abdul-Khaliq Sakinah , Cadet Chris E. A. , Hendry Tory A.
Published: December 2023

Bacterial communities in the phyllosphere, the above-ground parts of plants, are diverse yet understudied. These bacteria are important for plant health and also for inter-kingdom interactions with beneficial and pest insect species. Here, we present draft genomes of eight culturable bacterial isolates from leaf surfaces in the
Pisum sativum
phyllosphere.
Supplemental Material: Evolution of an earthquake-induced landslide complex in the South Island of New Zealand: How fault damage zones and seismicity contribute to slope failures
Authors: Singeisen Corinne , al. et
Published: December 2023

Ground model provides a 3-D model of the landslide including the two geophysical profiles and interpretation of landslide failure mechanism. The model can be viewed using the free desktop application Leapfrog viewer, which can be downloaded from https://www.seequent.com/products-solutions/leapfrog-viewer/. 
China’s Foreign Policy toward the Central Asian Republics through the BRI
Authors: Yazdani Enayatollah , Zeng Ji
Published: December 2023

Central Asia, located in Eurasia’s hinterland, is one of China’s primary foreign policy destinations. The Belt and Road Initiative (BRI) has emerged as the most important means of furthering relations between China and the five Central Asian republics. A new phase in China’s foreign policy towards Central Asia was marked by the launch of the BRI, which sparked a new round of cooperation between China and the Central Asian republics. Since the implementation of the BRI in 2013 China’s foreign policy has prioritized Central Asia as part of the initiative. China’s economic interests and influence in the Central Asian republics have increased as a result of the initiative’s implementation. In the era of the multipolar world order in which China as one of the major powers is playing a central role, Beijing’s international policy includes its bilateral and multilateral relations with the Central Asian states. This paper aims to discuss how the Chinese BRI has reconfigured China’s exchange relations and influences on Central Asian countries, as well as how the BRI has worked in each country in the region.
Multi-SpacePhish: Extending the Evasion-space of Adversarial Attacks against Phishing Website Detectors Using Machine Learning
Authors: Yuan Ying , Apruzzese Giovanni , Conti Mauro
Published: December 2023

Existing literature on adversarial Machine Learning (ML) focuses either on showing attacks that break every ML model or defenses that withstand most attacks. Unfortunately, little consideration is given to the actual
feasibility
of the attack or the defense. Moreover, adversarial samples are often crafted in the “feature-space,” making the corresponding evaluations of questionable value. Simply put, the current situation does not allow one to estimate the actual threat posed by adversarial attacks, leading to a lack of secure ML systems.

We aim to clarify such confusion in this article. By considering the application of ML for Phishing Website Detection (PWD), we formalize the “evasion-space,” in which an adversarial perturbation can be introduced to fool an ML-PWD—demonstrating that even perturbations in the “feature-space” are useful. Then, we propose a realistic threat model describing evasion attacks against ML-PWD that are cheap to stage, and hence intrinsically more attractive for real phishers. After that, we perform the first statistically validated assessment of state-of-the-art ML-PWD against 12 evasion attacks. Our evaluation shows (i) the true efficacy of evasion attempts that are more likely to occur; and (ii) the impact of perturbations crafted in different evasion-spaces; our realistic evasion attempts induce a statistically significant degradation (3–10% at
p
< 0.05), and their cheap cost makes them a subtle threat. Notably, however, some ML-PWD are immune to our most realistic attacks (
p
= 0.22).

Finally, as an additional contribution of this journal publication, we are the first to propose and empirically evaluate the intriguing case wherein an attacker introduces perturbations in multiple evasion-spaces
at the same time
. These new results show that simultaneously applying perturbations in the problem- and feature-space can cause a drop in the detection rate from 0.95 to 0.

Our contribution paves the way for a much-needed re-assessment of adversarial attacks against ML systems for cybersecurity.
Role of the CD40-CD40 Ligand Pathway in Cardiovascular Events, Neurological Alterations, and Other Clinical Complications of Chronic Hemodialysis Patients: Protective Role of Adsorptive Membranes
Authors: Marengo Marita , Migliori Massimiliano , Merlotti Guido , Fabbrini Paolo , Panichi Vincenzo , Cantaluppi Vincenzo
Published: December 2023

Despite the recent advances in dialysis technology, mortality rate of chronic uremic patients still remains excessively high: of note, in comparison to age- and sex-matched healthy controls, this frail population shows a higher incidence of infections, cancer, cognitive decline, and, in particular, major adverse cardiovascular events (MACE) that represent nowadays the first cause of mortality. Several traditional and nontraditional factors contribute to this increased risk for MACE and accelerated cellular senescence: among these, inflammation has been shown to play a key role. The costimulatory pathway CD40-CD40 Ligand (CD40L) is harmfully activated during inflammation and uremia-associated clinical complications: in particular, the soluble form of CD40L (sCD40L) can bind to the CD40 receptor triggering a cascade of detrimental pathways in immune and nonimmune cells. In this narrative review, we summarize the current concepts of the biological role of the CD40-CD40L pathway in uremia-associated organ dysfunction, focusing on the above-described main causes of mortality. Moreover, we discuss the interaction of the CD40-CD40L pathway with extracellular vesicles, microparticles recently identified as new uremic toxins. The biological effects of sCD40L in MACE, cognitive decline, infections, and cancer will be also briefly commented. Last, based on recent studies and ongoing clinical trials, we herein describe the modulatory activity of adsorptive dialysis membranes in polymethylmethacrylate on CD40-CD40L detrimental activation.
Arteriolar Hyalinosis Predicts the Onset of Both Macroalbuminuria and Impaired Renal Function in Patients with Type 2 Diabetes
Authors: Suzuki Akihiko , Moriya Tatsumi , Hayashi Akinori , Matsubara Madoka , Miyatsuka Takeshi
Published: December 2023

Introduction: Arteriolar hyalinosis (AH) has been shown to be associated with albuminuria and GFR. In this study, we investigated whether or not index of AH (IAH) is a predictor of the onset of macroalbuminuria and impaired renal function (eGFR <60 mL/min/1.73 m2 [eGFR <60]) in type 2 diabetic patients with early diabetic nephropathy. Methods: The study population consisted of 35 patients with type 2 diabetes (25 men; age: 47 ± 9 years; eGFR: 92.7 ± 18.0 mL/min/1.73 m2) with normo- or microalbuminuria who underwent percutaneous renal biopsy. These patients were followed for at least 5 (18 ± 6, range: 6–28) years. The study endpoint was the onset of macroalbuminuria or eGFR <60. Light and electron microscopy-based morphometric analyses were performed to quantitatively evaluate glomerular and interstitial structural changes. Results: During the observation period, 9 out of the 35 patients progressed to macroalbuminuria, and 15 out of the 35 patients developed eGFR <60. The annual rate of eGFR decline was significantly correlated with IAH (r = −0.40, p = 0.016). Kaplan-Meier analysis demonstrated that AH was associated with a significantly higher risk of onset of macroalbuminuria and eGFR <60, and microalbuminuria is associated with the onset of macroalbuminuria but not the onset of eGFR <60. Conclusions: Aggravated AH is a histological risk factor which predicts the onset of macroalbuminuria and eGFR <60 in patients with type 2 diabetes. These findings provide novel insights into the mechanism of progression of diabetic nephropathy.
Association of Serum Free Light Chain Level with Long-Term Kidney Function in Patients with Newly Diagnosed Multiple Myeloma
Authors: Lopedote Paolo , Alhaddad Juliano , Zheng Guoliang , Abualshar Mu’taz , Ghanta Shree , Kozyreva Olga , Jaber Bertrand L.
Published: December 2023

Introduction: Multiple myeloma (MM) frequently involves the kidneys, resulting in acute, subacute, or chronic kidney disease (CKD). Patient- and treatment-related factors are associated with the long-term development of CKD. The aim of our study was to examine the association of serum free light chain (FLC) levels, measured at the time of diagnosis of MM, and CKD at subsequent follow-up. Methods: Patients with newly diagnosed MM were identified using cancer registries at five hospitals. The primary outcome was low eGFR (<60 mL/min/1.73 m2) or dialysis dependence and a secondary composite outcome of low eGFR, dialysis dependence, or death at the last follow-up, up to 12 months from diagnosis. Logistic regression analyses were performed. Results: A total of 149 patients met the inclusion criteria. Patients with an FLC level above the median had a higher frequency of hypertension (54% vs. 81%; p < 0.001), hyperlipidemia (37% vs. 56%; p = 0.018), low eGFR at the time of diagnosis (43% vs. 66%; p = 0.006), and a higher MM stage (p = 0.018). On multivariable analyses, after adjustment for several covariates, serum FLC level (per each 100 mg/L) was independently associated with low eGFR or dialysis dependence at follow-up (adjusted odds ratio [aOR] 1.021; 95% CI: 1.002, 1.041; p = 0.033). This association persisted for the composite outcome of low eGFR, dialysis dependence, or death (aOR 1.034; 95% CI: 1.006, 1.063; p = 0.018). Discussion/Conclusion: Higher serum FLC level measured at the time of MM diagnosis is independently associated with CKD at up to 12 months of follow-up.
Prodromus of a fern flora of Bolivia. XLII. Update I.
Authors: KESSLER MICHAEL , SMITH ALAN R. , ØLLGAARD BENJAMIN , MATOS FERNANDO B. , MORAN ROBBIN C.
Published: December 2023

We here provide a taxonomic and nomenclatural update of the Prodromus of the Bolivian fern flora published between 2017 and 2019. We describe one new species (Elaphoglossum neglectum F.B.Matos & R.C.Moran), provide four new combinations (Argyrochosma flavens (Sw.) A.R.Sm. & M.Kessler, Ceradenia werffii (L.E.Bishop) A.R.Sm. & M.Kessler, Enterosora dudleyi (L.E.Bishop) A.R.Sm. & M.Kessler, Phlegmariurus aristei (Nessel) B.Øllg.), report 13 new species records for Bolivia, delete 4 species from the Bolivian list, and update the names of 17 species previously known from Bolivia. With these new records and species, the number of fern and lycophyte species known from Bolivia increases by 10 to 1210.
Revealing new records of hypocrealean fungicolous fungi from northern Thailand
Authors: GAJANAYAKE ACHALA J. , JAYAWARDENA RUVISHIKA S. , LUANGHARN THATSANEE
Published: December 2023

Hypocreales comprise a diverse group of fungi that exhibit a wide range of lifestyles in both aquatic and terrestrial environments. A significant number of the identified fungicolous taxa belong to Hypocreales. Samples were collected from Chiang Rai and Chiang Mai Provinces during an investigation on fungicolous fungi in northern Thailand. Based on morphological characteristics and multi-gene phylogenetic analyses of ITS-LSU-tef1α-rpb2 and ITS-LSU-tub2-tef1α, two isolates were identified as Hypomyces sichuanensis (Hypocreaceae) and Mariannaea humicola (Nectriaceae) respectively. This study marks the first documentation of Hypomyces sichuanensis and Mariannaea humicola as fungicolous species from Thailand. Additionally, the study reveals a novel host record for M. humicola on Marasmius sp.
Characters evolution of Encyclia (Laeliinae-Orchidaceae) reveals a complex pattern not phylogenetically determined: insights from macro- and micromorphology
Authors: Lipińska Monika M. , Olędrzyńska Natalia , Dudek Magdalena , Naczk Aleksandra M. , Łuszczek Dorota , Szabó Peter , Speckmaier Manfred , Szlachetko Dariusz L.
Published: December 2023

Encyclia is the second-largest genus in the neotropical subtribe Laeliinae (Orchidaceae) and has more than 150 species, which are characterized by fairly consistent flower morphology. Its taxonomy and species boundaries, however, seem to be still under debate. In the present study, we first examined the lip micromorphology of 61 species of Encyclia sensu stricto. We correlated our results with external flower morphology and phylogenetic analyses performed on a combined dataset that included both nuclear (ITS, Xdh, PhyC) and plastid markers (ycf1, rpl32, and trnL-trnF). Phylogenetic reconstruction showed that Encyclia sensu stricto species form a coherent, monophyletic group. However, it is difficult to determine the relationships between the different groups within one larger clade. The groups all form distinct lineages that evolved from a common ancestor. The UPGMA cluster analysis for the seven qualitative micromorphological features clearly divides the genus into two main groups, the larger of which is further subdivided into two subgroups. None of these, however, overlap with any of the phylogeographic units distinguished in previously published papers or in presented article. It is worth noting that the groups resulting from the UPGMA analysis cannot be defined by macromorphological features. The pattern of similarities between species, taking into account both macro- and micromorphological features, is eminently mosaic in nature, and only a multifaceted approach can explain this enigmatic group.
Genomic exploration of Sesuvium sesuvioides: comparative study and phylogenetic analysis within the order Caryophyllales from Cholistan desert, Pakistan
Authors: Javaid Nida , Ramzan Musarrat , Jabeen Shagufta , Shah Muhammad Nadeem , Danish Subhan , Hirad Abdurahman Hajinur
Published: December 2023

Background

The Aizoaceae family’s Sesuvium sesuvioides (Fenzl) Verdc is a medicinal species of the Cholistan desert, Pakistan. The purpose of this study was to determine the genomic features and phylogenetic position of the Sesuvium genus in the Aizoaceae family. We used the Illumina HiSeq2500 and paired-end sequencing to publish the complete chloroplast sequence of S. sesuvioides.

Results

The 155,849 bp length cp genome sequence of S. sesuvioides has a 36.8% GC content. The Leucine codon has the greatest codon use (10.6%), 81 simple sequence repetitions of 19 kinds, and 79 oligonucleotide repeats. We investigated the phylogeny of the order Caryophyllales’ 27 species from 23 families and 25 distinct genera. The maximum likelihood tree indicated Sesuvium as a monophyletic genus, and sister to Tetragonia. A comparison of S. sesuvioides, with Sesuvium portulacastrum, Mesembryanthemum crystallinum, Mesembryanthemum cordifolium, and Tetragonia tetragonoides was performed using the NCBI platform. In the comparative investigation of genomes, all five genera revealed comparable cp genome structure, gene number and composition. All five species lacked the rps15 gene and the rpl2 intron. In most comparisons with S. sesuvioides, transition substitutions (Ts) were more frequent than transversion substitutions (Tv), producing Ts/Tv ratios larger than one, and the Ka/Ks ratio was lower than one. We determined ten highly polymorphic regions, comprising rpl22, rpl32-trnL-UAG, trnD-GUC-trnY-GUA, trnE-UUC-trnT-GGU, trnK-UUU-rps16, trnM-CAU-atpE, trnH-GUG-psbA, psaJ-rpl33, rps4-trnT-UGU, and trnF-GAA-ndhJ.

Conclusion

The whole S. sesuvioides chloroplast will be examined as a resource for in-depth taxonomic research of the genus when more Sesuvium and Aizoaceae species are sequenced in the future. The chloroplast genomes of the Aizoaceae family are well preserved, with little alterations, indicating the family’s monophyletic origin. This study’s highly polymorphic regions could be utilized to build realistic and low-cost molecular markers for resolving taxonomic discrepancies, new species identification, and finding evolutionary links among Aizoaceae species. To properly comprehend the evolution of the Aizoaceae family, further species need to be sequenced.
Notch3 restricts metastasis of breast cancers through regulation of the JAK/STAT5A signaling pathway
Authors: Chen Min-Na , Fang Ze-Xuan , Wu Zheng , Bai Jing-Wen , Li Rong-Hui , Wen Xiao-Fen , Zhang Guo-Jun , Liu Jing
Published: December 2023

Purpose

To explore the potential role of signal transducer and activator of transcription 5A (STAT5A) in the metastasis of breast cancer, and its mechanism of regulation underlying.

Methods and results

TCGA datasets were used to evaluate the expression of STAT5A in normal and different cancerous tissues through TIMER2.0, indicating that STAT5A level was decreased in breast cancer tissues compared with normal ones. Gene Set Enrichment Analysis predicted that STAT5A was associated with the activation of immune cells and cell cycle process. We further demonstrated that the infiltration of immune cells was positively associated with STAT5A level. Influorescence staining revealed the expression and distribution of F-actin was regulated by STAT5A, while colony formation assay, wound healing and transwell assays predicted the inhibitory role of STAT5A in the colony formation, migratory and invasive abilities in breast cancer cells. In addition, overexpression of the Notch3 intracellular domain (N3ICD), the active form of Notch3, resulted in the increased expression of STAT5A. Conversely, silencing of Notch3 expression by siNotch3 decreased STAT5A expression, supporting that STAT5A expression is positively associated with Notch3 in human breast cancer cell lines and breast cancer tissues. Mechanistically, chromatin immunoprecipitation showed that Notch3 was directly bound to the STAT5A promoter and induced the expression of STAT5A. Moreover, overexpressing STAT5A partially reversed the enhanced mobility of breast cancer cells following Notch3 silencing. Low expression of Notch3 and STAT5A predicted poorer prognosis of patients with breast cancer.

Conclusion

The present study demonstrates that Notch3 inhibits metastasis in breast cancer through inducing transcriptionally STAT5A, which was associated with tumor-infiltrating immune cells, providing a novel strategy to treat breast cancer.
Characterization of postoperative LASIK ectasia features on higher-order aberration excimer ablation maps
Authors: Wallerstein Avi , Santhakumaran Sangeetha , Tabunar Lauren , Cohen Mark , Gauvin Mathieu
Published: December 2023

Background

To characterize anterior corneal higher-order aberration (HOA) excimer ablation map patterns in postoperative LASIK ectasia (POE) and to examine correlations between newly identified corneal HOA ablation map features of POE and known topographic indices.

Methods

Prospective multicenter non-interventional descriptive study. A total of 28 eyes from 22 POE patients were enrolled. The postoperative HOA ablation map was derived from Topolyzer Vario corneal imaging at the time of POE diagnosis. Features that recurred were identified and then analyzed. Correlations to Orbscan indices were studied.

Results

An arrangement of two elliptical paracentral ablation islands, deep inferior and shallow superior, in direct mirror-like opposition to each other, were identified on all HOA maps. The paracentral islands were accompanied by peripheral ablation crescents. The deep paracentral inferior island ‘hot spot’ coincided with the topographical apical POE cone and was highly reproducible in angular position (249.3 ± 17.3°). There was significant variation in ablation depth (shallow superior island: 11.5 ± 6.9 μm and deep inferior island: 32.5 ± 18.8 μm). The superior crescents had high variability in depth (34.8 ± 18.9 μm). Strong correlations were found between the corneal irregularity index and the ablation depth difference between the deep and shallow paracentral islands (R = 0.96; P < 0.0001).

Conclusion

The corneal HOA excimer ablation map revealed a recurring, distinct, easily recognizable pattern in POE eyes. Validated Orbscan POE indices and HOA ablation map islands showed a strong correlation. It is possible to extract useful information from the corneal HOA ablation map, potentially making it suitable for diagnosing and monitoring POE although more studies are needed.
Oral health-related quality of life and depressive symptoms in adults: longitudinal associations of the English Longitudinal Study of Ageing (ELSA)
Authors: Zwick Luisa , Schmitz Norbert , Shojaa Mahdieh
Published: December 2023

Background

Little is known about the relationship between oral health status and depressive symptoms in adults in England. The aim of this study was to examine the longitudinal association between oral health parameters and depressive symptoms in adults in England.

Methods

Data were obtained from the English Longitudinal Study of Aging (ELSA), which included information on self-rated oral health, oral impairment in daily life (Oral Impacts on Daily Performances, OIDP), and depressive symptoms (Center for Epidemiologic Studies Depression Scale, CES-D) in 6790 adults aged ≥ 50 years. Wave 3 data were used as baseline, while Waves 5 and 7 were used for follow-up assessments. Logistic regression was used to determine whether depressive symptoms at baseline anticipated self-rated oral health and OIDP and whether oral health status (at baseline) was associated with the development of depressive symptoms at follow-up assessment.

Results

Participants with poor self-rated oral health were at higher risk of developing depressive symptoms, even after adjusting for behavioral, clinical, and sociodemographic characteristics (OR = 1.69, 95% CI 1.38–2.07). Similarly, having oral impacts on daily performances were associated with the development of depressive symptoms: The OR for developing depressive symptoms at Wave 5 or 7 was 2.19 (95% CI 1.62–2.96) after adjustment for all covariates. Participants with depressive symptoms at baseline were more likely to report poor self-rated oral health (OR = 1.93, 95% CI 1.52–2.44) or one or more oral impacts (OR = 1.86, 95% CI 1.45–2.40) at follow-up than those without depressive symptoms at baseline, even after adjusting for confounders.

Conclusions

In the present study, a bidirectional association was found between depressive symptoms and poor oral health in older adults. Maintaining good oral health in older adults may be a protective factor against depressive symptoms. Therefore, more attention should be paid to promoting oral health awareness in older adults, including encouraging regular dental checkups, proper toothbrushing and flossing techniques, and healthy lifestyles.
Attrition of health extension workers in Ethiopia: trends, regional variations and determinants – a mixed methods study of 15 years of experience
Authors: Wolde Habtamu Milkias , Tekle Merhawi Gebremedhin , Alemayehu Yibeltal Kiflie , Gebre Esie Gebrewahd , Teklu Alula M.
Published: December 2023

Background

Ensuring regular supervision, capacity building and motivation are crucial for the successful retention of health extension workers (HEWs). Failure in these aspects could increase the attrition rate of HEWs. To date, there has not been a comprehensive nationwide study on HEW attrition that could act as a source of evidence for policy makers. This study explored HEW attrition, including leaving the health sector entirely and its regional variation, trends and predictors of attrition out of the health system.

Methods

This study explored the attrition of HEWs from the beginning of the program until the end of 2018. A district-based mixed method study was conducted to review the personnel files of HEWs. A multistage sampling technique was employed to select 3,476 HEWs, and a probability weight was assigned for each observation. Descriptive statistics were calculated for the outcome and predictor variables. A logistic regression model was used to model attrition out of the health system. A qualitative study was conducted to understand the reasons why HEWs leave their jobs. Thematic analysis was performed using Nvivo version 12.

Results

The magnitude of attrition of HEWs was found to be 21.1% during the fifteen years of HEP implementation. Of the total 704 who left their job as an HEW, 530 (73%) left the health system altogether. Number of biological children [AOR = 0.61, 95% CI; 0.42–0.89], having an additional education [AOR = 8.34, 95% CI; 3.67–18.98], obtaining official recognition [AOR = 0.29, 95% CI; 0.10–0.83], administrative reprimand [AOR = 1.66, 95% CI; 1.07 -2. 56), distance between district health office and health post [AOR = 1.75, 95%CI; 1.18–2.59) and COC status [AOR = 2.06, 95%CI 1.39–3.06) were independent predictors of leaving the health sector. High regional variation in attrition was observed, ranging from 38.5% in Addis Ababa to just 6.1% in the Harari region. The trend of attrition has steadily increased over time, with a high of 1,999 attritions per 10,000 HEW in 2018. Psychosocial factors, administrative issues, career advancement incentives, and workplace-related problems were the themes that emerged from the qualitative study as reasons for attrition of HEWs.

Conclusion

Even though the magnitude of attrition was relatively low, there was high regional variation and incremental trends. Moreover, the out-of-health sector attrition is also high. Critically examining the HEP policy environment to increase the number of HEWs deployed per health post to reduce workload and improving HEW incentives, including career development, may assist in increasing HEW job satisfaction, which in turn could help to reduce attrition, including leaving the health sector.
Perceptions of general practitioners towards the services provided by advanced practice nurses: a cross-sectional survey in France
Authors: Goddaert Charles , Gérard Pierre-Antoine , Kessler Charlotte , Leblanc Mélaine , Barbe Coralie , Chrusciel Jan , Cormi Clément , Sanchez Stéphane
Published: December 2023

Background

New healthcare professions are emerging due to scarce medical resources. The appearance of a new healthcare profession, advanced practice nurses (APNs), has raised questions about how general practitioners interrelate with them as primary care providers. The objective of this study was to explore the perceptions general practitioners have towards the services rendered by APNs to patients, to general practice and the role they play in the healthcare system.

Methods

A survey-based, cross-sectional study was conducted throughout the Grand Est region of France which covers 57,333km2 and has a population of approximately 5,562,651. The survey was compiled using pre-existing questionnaires and was carried out from July to September 2022 via email. Variables collected were rate of acceptability and socio-demographic characteristics.

Results

In total, 251 responses were included. The mean age of general practitioners was 41.7 years, most were women (58.2%) and worked in rural areas of the region (53.8%). Over 80% of respondents practiced in group structures (defined as either multi-professional health centers (n = 61) or in group practices (n = 143)). Most respondents (94.0%) were familiar with the APN profession and did not consider that APNs improved access to care (55.8%, percent of responders with score ≤ 3/10). Moreover, most did not believe that APNs were useful as a primary care provider for patients (61.8%). However, being a member of a territorialized healthcare community, known as Communautés Professionnelles Territoriales de Santé (CPTS), was associated with a positive appraisal of APNs’ services (OR = 2.116, 95%CI: 1.223 to 3.712; p = 0.007).

Conclusions

Encouraging shared and networked practice within a healthcare community may promote a positive perception of new actors. Further studies need to be conducted to show whether the integration of APNs into healthcare networks improves quality of care.
A colorimetric, photothermal, and fluorescent triple-mode CRISPR/cas biosensor for drug-resistance bacteria detection
Authors: Zheng Laibao , Jiang Yayun , Huang Fuyuan , Wu Qiaoli , Lou Yongliang
Published: December 2023

A multimodal analytical strategy utilizing different modalities to cross-validate each other, can effectively minimize false positives or negatives and ensure the accuracy of detection results. Herein, we establish a colorimetric, photothermal, and fluorescent triple modal CRISPR/Cas12a detection platform (CPF-CRISPR). An MNPs-ssDNA-HRP signal probe is designed to act as a substrate to trigger three signal outputs. In the presence of the DNA target, MNPs-ssDNA-HRP is cleaved by the activated CRISPR/Cas12a, resulting in the release of HRP and generating short DNA strands with 3-terminal hydroxyl on magnetic beads. The released HRP subsequently catalyzed TMB-H2O2 reaction and oxidized TMB is used for colorimetric and photothermal signal detection. Under the catalysis of terminal deoxynucleotidyl transferase (TdT), the remaining short DNA strands are used as primers to form poly-T and function as scaffolds to form copper nanoclusters for fluorescent signal output. To verify the practical application of CPF-CRISPR, we employed MRSA as a model. The results demonstrate the platform’s high accuracy and sensitivity, with a limit of detection of 101 CFU/mL when combined with recombinase polymerase amplification. Therefore, by harnessing the programmability of CRISPR/Cas12a, the biosensor has the potential to detect various drug-resistant bacteria, demonstrating significant practical applicability.
How can civil society organizations contribute to the scale-up of comprehensive sexuality education? Presentation of a scaling framework illustrated with examples from Indonesia
Authors: Kockelkoren Ardan , Rahmah Amala , Pangestu Muhammad Rey Dwi , Sawitri Ely , Widyastuti Elisabet Setya Asih , Astiti Ni Luh Eka Purni , Michielsen Kristien , Van Reeuwijk Miranda
Published: December 2023

Comprehensive sexuality education (CSE) can substantially contribute to the health and well-being of young people. Yet, most CSE interventions remain limited to the small piloting or research phase and scale-up is often an afterthought at the end of a project. Because of the specificities of CSE, including it being a controversial topic in many contexts and a topic on the fringe between health, education and youth, a specific scaling approach to CSE is needed. The commentary presents a practical framework to support civil society organisations (CSOs), to address barriers to scaling up CSE in their contexts. The utilization and relevance of the framework is demonstrated in this article, by featuring examples from the scale up process of CSE in Indonesia. The framework identifies key principles for scaling up, including: taking a scaling mindset from the start, government ownership and political commitment for scale-up, and identifying the added value of CSOs. The framework starts with a self-assessment by the CSO and then follows four phases: making the case, engaging in dialogue, establishing building blocks and implementation and scale-up. Each of these phases are illustrated with examples from Indonesia.

This framework is a call to action with practical guidelines to support CSOs to take on this role, because with the right scaling strategies, the largest generation of young people ever alive can become healthy, empowered and productive adults.
Post-migration stressors, mental health and well-being in resettled refugees from Syria: Do individuals’ coping strategies matter?
Authors: Solberg Øivind , Nissen Alexander , Saboonchi Fredrik
Published: December 2023

Background

The evidence is mixed as to whether individuals’ coping strategies may mitigate the adverse mental health effects of post-displacement stressors in refugee populations, with some indications that the buffering effects of coping strategies are context dependent. The present study examined if problem-solving and acceptance coping strategies were effect modifiers between post-migration stressors and mental health in adult refugees from Syria resettled in Sweden.

Methods

Study aims were investigated using cross-sectional survey data from a nationwide, randomly sampled group of adult refugees from Syria granted permanent residency in Sweden between 2011 and 2013 (Nsample = 4000, nrespondents = 1215, response rate 30.4%). Post-migration stressors examined included: financial strain, social strain, host-country competency strain and discrimination. Two mental health outcomes were used: anxiety/depression, measured with the Hopkins Symptom Checklist-25; and well-being, measured with the WHO-5 Well-being Index. Both outcomes were modelled continuously. Coping strategies were measured using the BRIEF Cope scale. Interactions between coping strategies and post-migration stressors were tested in fully adjusted linear regression models using Wald test for interaction, corrected for multiple testing using the Benjamini–Hochberg procedure.

Results

Both problem-solving and acceptance coping strategies buffered the adverse association between financial strain and symptoms of anxiety/depression, and problem-solving coping strategies buffered the adverse association between host-country competency strain and anxiety/depression.

Conclusions

The study suggests that individuals’ coping strategies may to some degree buffer the adverse mental health effects of financial strain and host-country competency strain experienced by refugees in the resettlement phase. Although this pattern was only found in regard to anxiety/depression and not subjective well-being, the findings show that individual-level coping skills among refugees may contribute to adaptation in the face of post-settlement adversities. Notwithstanding the importance of attending to refugees’ psychosocial conditions, refugees residing in refugee camps and newly resettled refugees might benefit from interventions aiming at enhancing individual coping resources and skills. The potential effect of increased controllability and decreased conflict-proximity also warrants further exploration in future studies.
Total laryngectomy and readmission: causes, rates and predictors
Authors: Rammal Almoaidbellah , Alqutub Abdulsalam , Alsulami Omar , Mozahim Naif , Mozahim Sara , Awadh Mohammed , Hakami Muatasaim , AlThomali Rahaf , Mogharbel Ahmed
Published: December 2023

Background

Total laryngectomy (TL) is a complex procedure, and patients undergoing TL are at high risk for readmission, which exposes them to hospital-acquired complications. Readmission rate is a metric for quality of care. We aimed to identify the rate, causes, and predictors of hospital readmission within 60 days after discharge following TL.

Methods

This is a 12-year retrospective study where we included all patients undergoing TL in a single tertiary care center between 2008 and 2022. Patient charts were reviewed for demographics, comorbidities, and causes for readmission.

Results

Of 83 patients who underwent TL, 12 (14.50%) were readmitted within 60 days. Common causes were surgical site infection (33.33%) and mucocutaneous fistula (25%). Significant predictors for readmission were tobacco use (P = 0.003), African ethnicity (P = 0.004), being unmarried (P < 0.001), lower preoperative serum albumin (P < 0.001), higher preoperative TSH (P = 0.03), higher preoperative neutrophil count (P = 0.035), higher American Society of Anesthesiology (ASA) score (P = 0.028), and higher Cumulative Illness Rating Scale (CIRS) score (P = 0.029).

Conclusion

One in every seven patients were readmitted following TL. Frequent causes include wound infection and fistulas. Predictors include preoperative hypoalbuminemia, hypothyroidism, African ethnicity, being unmarried, tobacco use, and a higher baseline burden of comorbidities. Such factors can be targeted to reduce hospital readmission rates.