bluuebunny/crossref_metadata_2025 huggingface dataset:
Topoisomerase poisoning by the flavonoid nevadensin triggers DNA damage and apoptosis in human colon carcinoma HT29 cells
Authors: Müller Lena , Schütte Larissa Rhonda Friederike , Bücksteeg David , Alfke Julian , Uebel Thomas , Esselen Melanie
Published: October 2021

Nevadensin, an abundant polyphenol of basil, is reported to reduce alkenylbenzene DNA adduct formation. Furthermore, it has a wide spectrum of further pharmacological properties. The presented study focuses the impact of nevadensin on topoisomerases (TOPO) in vitro. Considering the DNA-intercalating properties of flavonoids, first, minor groove binding properties (IC50 = 31.63 µM), as well as DNA intercalation (IC50 = 296.91 µM) of nevadensin, was found. To determine potential in vitro effects on TOPO I and TOPO IIα, the relaxation and decatenation assay was performed in a concentration range of 1–500 µM nevadensin. A partial inhibition was detected for TOPO I at concentrations  ≥ 100 µM, whereas TOPO IIα activity is only inhibited at concentrations  ≥ 250 µM. To clarify the mode of action, the isolating in vivo complex of enzyme assay was carried out using human colon carcinoma HT29 cells. After 1 h of incubation, the amount of TOPO I linked to DNA was significantly increased by nevadensin (500 µM), why nevadensin was characterized as TOPO I poison. However, no effects on TOPO IIα were detected in the cellular test system. As a subsequent cellular response to TOPO I poisoning, a highly significant increase of DNA damage after 2 h and a decrease of cell viability after 48 h at the same concentration range were found. Furthermore, after 24 h of incubation a G2/M arrest was observed at concentrations ≥ 100 µM by flow cytometry. The analysis of cell death revealed that nevadensin induces the intrinsic apoptotic pathway via activation of caspase-9 and caspase-3. The results suggest that cell cycle disruption and apoptotic events play key roles in the cellular response to TOPO I poisoning caused by nevadensin in HT29 cells.
Cyclodextrin-assisted surface-enhanced Raman spectroscopy: a critical review
Authors: Markina Natalia E. , Cialla-May Dana , Markin Alexey V.
Published: October 2021

Numerous approaches have been proposed to overcome the intrinsically low selectivity of surface-enhanced Raman spectroscopy (SERS), and the modification of SERS substrates with diverse recognition molecules is one of such approaches. In contrast to the use of antibodies, aptamers, and molecularly imprinted polymers, application of cyclodextrins (CDs) is still developing with less than 100 papers since 1993. Therefore, the main goal of this review is the critical analysis of all available papers on the use of CDs in SERS analysis, including physicochemical studies of CD complexation and the effect of CD presence on the Raman enhancement. The results of the review reveal that there is controversial information about CD efficiency and further experimental investigations have to be done in order to estimate the real potential of CDs in SERS-based analysis.

Graphical abstract
Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella
Authors: Rempfer Christine , Wiedemann Gertrud , Schween Gabriele , Kerres Klaus L. , Lucht Jan M. , Horres Ralf , Decker Eva L. , Reski Ralf
Published: October 2021

Key message

In Physcomitrella, whole-genome duplications affected the expression of about 3.7% of the protein-encoding genes, some of them relevant for DNA repair, resulting in a massively reduced gene-targeting frequency.

Qualitative changes in gene expression after an autopolyploidization event, a pure duplication of the whole genome (WGD), might be relevant for a different regulation of molecular mechanisms between angiosperms growing in a life cycle with a dominant diploid sporophytic stage and the haploid-dominant mosses. Whereas angiosperms repair DNA double-strand breaks (DSB) preferentially via non-homologous end joining (NHEJ), in the moss Physcomitrella homologous recombination (HR) is the main DNA–DSB repair pathway. HR facilitates the precise integration of foreign DNA into the genome via gene targeting (GT). Here, we studied the influence of ploidy on gene expression patterns and GT efficiency in Physcomitrella using haploid plants and autodiploid plants, generated via an artificial WGD. Single cells (protoplasts) were transfected with a GT construct and material from different time-points after transfection was analysed by microarrays and SuperSAGE sequencing. In the SuperSAGE data, we detected 3.7% of the Physcomitrella genes as differentially expressed in response to the WGD event. Among the differentially expressed genes involved in DNA–DSB repair was an upregulated gene encoding the X-ray repair cross-complementing protein 4 (XRCC4), a key player in NHEJ. Analysing the GT efficiency, we observed that autodiploid plants were significantly GT suppressed (p < 0.001) attaining only one third of the expected GT rates. Hence, an alteration of global transcript patterns, including genes related to DNA repair, in autodiploid Physcomitrella plants correlated with a drastic suppression of HR.
Yes, they can: polar bears Ursus maritimus successfully hunt Svalbard reindeer Rangifer tarandus platyrhynchus
Authors: Stempniewicz Lech , Kulaszewicz Izabela , Aars Jon
Published: October 2021

The polar bearUrsus maritimusis one of the species most endangered by the rapidly declining sea–ice cover in the Arctic, which they use as a platform to hunt fatty, high-energy seals. In recent decades, more polar bears have been forced to remain longer on land, so their access to seals is limited. The importance of terrestrial food to polar bears is disputable, and more data are needed. Terrestrial ungulates could be an attractive substitute prey for them. Svalbard reindeerRangifer tarandus platyrhynchusare prevalent and their distribution is completely within the range of polar bears. They constitute an attractive potential prey offering a significant energy return. Pre-2000 sources state that polar bears do not attack Svalbard reindeer. This report is the first description and documentation of the complete course of a polar bear hunt for adult reindeer in Hornsund, SW Spitsbergen, and also of the bear’s hunting behaviour and the reindeer’s response. Further, we report several other recent instances of bear–reindeer interactions in Svalbard, suggesting that polar bears now hunt reindeer more frequently than they used to. This increase in hunting is probably linked to the reduced ice cover, with bears spending more time on land, and a growing reindeer population. This study adds to earlier papers on how polar bears in Svalbard have increasingly shifted to a more terrestrial diet, and indicates that they may have an enhanced role as an apex predator in the terrestrial ecosystem.
Retinal thinning in phenylketonuria and Gaucher disease type 3
Authors: Hopf Susanne , Schuster Alexander K. , Hennermann Julia B. , Pfeiffer Norbert , Pitz Susanne
Published: October 2021

Purpose

Retinal alterations in inherited metabolic diseases associated with neurodegeneration are poorly studied. The objective was to study retinal thickness, specifically the components of the ganglion cell complex (GCC)—nerve fiber layer (NFL), ganglion cell layer (GCL), and inner plexiform layer (IPL)—using spectral-domain optical coherence tomography (SD-OCT) in two different diseases with potential dopaminergic depletion, phenylketonuria (PKU) and Gaucher disease type 3 (GD3).

Methods

Retinal layers in 19 patients with PKU, 15 patients with GD3, and 93 healthy individuals were measured using peripapillary ring scan and macular SD-OCT. Linear mixed models were computed including an adjustment for age, sex, and spherical equivalent. We calculated Spearman’s rank correlations between retinal layer measurements and clinical and/or laboratory parameters.

Results

Thinning of total retinal thickness was found in the macular inner ring (p = 0.002), and outer ring (p = 0.012), sparing the fovea (p = 0.12) in PKU, while in GD3, all subfields were thinned (fovea p < 0.001, inner ring p = 0.047, outer ring 0.07). In both conditions, thinning was most evident in the NFL, GCL, and IPL, while OPL (outer plexiform layer) was thickened. Peripapillary retinal nerve fiber layer measurements remained normal. GCL and IPL in PKU correlated with tyrosine serum concentration.

Conclusion

Thinning of the NFL, GCL, and IPL, with thickened OPL, are both found in PKU and in GD3. Low dopamine concentrations in the retina might promote these effects. However, these data do not give evidence that retinal measurements can be used as a biomarker for disease severity in patients with GD3.
Diagnostic validity of early proximal caries detection using near-infrared imaging technology on 3D range data of posterior teeth
Authors: Litzenburger Friederike , Heck Katrin , Kaisarly Dalia , Kunzelmann Karl-Heinz
Published: October 2021

Objectives

This in vitro study analysed potential of early proximal caries detection using 3D range data of teeth consisting of near-infrared reflection images at 850 nm (NIRR).

Materials and methods

Two hundred fifty healthy and carious permanent human teeth were arranged pairwise, examined with bitewing radiography (BWR) and NIRR and validated with micro-computed tomography. NIRR findings were evaluated from buccal, lingual and occlusal (trilateral) views according to yes/no decisions about presence of caries. Reliability assessments included kappa statistics and revealed high agreement for both methods. Statistical analysis included cross tabulation and calculation of sensitivity, specificity and AUC.

Results

Underestimation of caries was 24.8% for NIRR and 26.4% for BWR. Overestimation was 10.4% for occlusal NIRR and 0% for BWR. Trilateral NIRR had overall accuracy of 64.8%, overestimation of 15.6% and underestimation of 19.6%. NIRR and BWR showed high specificity and low sensitivity for proximal caries detection.

Conclusions

NIRR achieved diagnostic results comparable to BWR. Trilateral NIRR assessments overestimated presence of proximal caries, revealing stronger sensitivity for initial caries detection than BWR.

Clinical relevance

NIRR provided valid complement to BWR as diagnostic instrument. Investigation from multiple angles did not substantially improve proximal caries detection with NIRR.
Quality of life and mental health in children and adolescents during the first year of the COVID-19 pandemic: results of a two-wave nationwide population-based study
Authors: Ravens-Sieberer Ulrike , Kaman Anne , Erhart Michael , Otto Christiane , Devine Janine , Löffler Constanze , Hurrelmann Klaus , Bullinger Monika , Barkmann Claus , Siegel Nico A. , Simon Anja M. , Wieler Lothar H. , Schlack Robert , Hölling Heike
Published: October 2021

Background

The COVID-19 pandemic has disrupted the lives of children and adolescents worldwide. The German COPSY study is among the first population-based longitudinal studies to examine the mental health impact of the pandemic. The objective of the study was to assess changes in health-related quality of life (HRQoL) and mental health in children and adolescents and to identify the associated risk and resource factors during the pandemic.

Methods

A nationwide longitudinal survey was conducted with two waves during the pandemic (May/June 2020 and December 2020/January 2021). In total, n = 1923 children and adolescents aged 7 to 17 years and their parents participated (retention rate from wave 1 to wave 2: 85%). The self-report and parent-proxy surveys assessed HRQoL (KIDSCREEN-10), mental health problems (SDQ with the subscales emotional problems, conduct problems, hyperactivity, and peer problems), anxiety (SCARED), depressive symptoms (CES-DC, PHQ-2) and psychosomatic complaints (HBSC-SCL). Mixed model panel regression analyses were conducted to examine longitudinal changes in mental health and to identify risk and resource factors.

Results

The HRQoL of children and adolescents decreased during the pandemic, and emotional problems, peer-related mental health problems, anxiety, depressive and psychosomatic symptoms increased over time, however the change in global mental health problems from wave 1 to wave 2 was not significant, and some changes were negligible. Socially disadvantaged children and children of mentally burdened parents were at particular risk of impaired mental health, while female gender and older age were associated with fewer mental health problems. A positive family climate and social support supported the mental health of children and adolescents during the pandemic.

Discussion

Health promotion, prevention and intervention strategies could support children and adolescents in coping with the pandemic and protect and maintain their mental health.
Support theorems for Funk-type isodistant Radon transforms on constant curvature spaces
Authors: Kurusa Árpád
Published: October 2021

A connected maximal submanifold in a constant curvature space is called isodistant if its points are in equal distances from a totally geodesic of codimension 1. The isodistant Radon transform of a suitable real function f on a constant curvature space is the function on the set of the isodistants that gives the integrals of f over the isodistants using the canonical measure. Inverting the isodistant Radon transform is severely overdetermined because the totally geodesic Radon transform, which is a restriction of the isodistant Radon transform, is invertible on some large classes of functions. This raises the admissibility problem that is about finding reasonably small subsets of the set of the isodistants such that the associated restrictions of the isodistant Radon transform are injective on a reasonably large set of functions. One of the main results of this paper is that the Funk-type sets of isodistants are admissible, because the associated restrictions of the isodistant Radon transform, we call them Funk-type isodistant Radon transforms, satisfy appropriate support theorems on a large set of functions. This unifies and sharpens several earlier results for the sphere, and brings to light new results for every constant curvature space.
Dual contextual module for neural machine translation
Authors: Ampomah Isaac Kojo Essel , McClean Sally , Hawe Glenn
Published: October 2021

Self-attention-based encoder-decoder frameworks have drawn increasing attention in recent years. The self-attention mechanism generates contextual representations by attending to all tokens in the sentence. Despite improvements in performance, recent research argues that the self-attention mechanism tends to concentrate more on the global context with less emphasis on the contextual information available within the local neighbourhood of tokens. This work presents the Dual Contextual (DC) module, an extension of the conventional self-attention unit, to effectively leverage both the local and global contextual information. The goal is to further improve the sentence representation ability of the encoder and decoder subnetworks, thus enhancing the overall performance of the translation model. Experimental results on WMT’14 English-German (En$$\rightarrow $$
→
De) and eight IWSLT translation tasks show that the DC module can further improve the translation performance of the Transformer model.
Relating matrix stress to local stress on a hard microstructural inclusion for understanding cleavage fracture in high strength steel
Authors: Jiang Quanxin , Bertolo V. M. , Popovich V. A. , Sietsma J. , Walters Carey L.
Published: October 2021

Macroscale cleavage fracture toughness of high strength steels is strongly related to the fracture of hard microstructural inclusions. Therefore, an accurate determination of the local stress on these inclusions based on the matrix stress is necessary for the statistical modelling of macroscale cleavage fracture. This paper presents analytical equations to quantitatively estimate the stress of the microstructural inclusions from the far-field stress of the matrix. The analytical equations account for the inclusion shape, the inclusion orientation, the far-field stress state and matrix material properties. Finite element modelling of a representative volume element containing a hard inclusion shows that the equations provide an accurate representation of the local stress state. The equations are implemented into a multi-barrier model and compared with CTOD experiments with two different levels of constraint.
Mathematics Assessment Practices of Primary School Teachers in France
Authors: Sayac Nathalie , Veldhuis Michiel
Published: October 2021

We investigated French primary school teachers’ assessment practice in mathematics. Using an online questionnaire on teachers’ background, teaching, and grading practice, we were able to determine assessment profiles of 604 primary school teachers. As evidenced by the teachers’ scores on the latent factors Assessment purposes, Assessment practices, and Differentiation, teachers with the profile of Enthusiastic assessors view assessment as more useful and use it more often to adapt their instruction than teachers with the profile of Unenthusiastic assessors. This can be useful for practice and sheds more light on French teachers’ assessment practices in mathematics. It is also interesting to compare the results of this survey with those from China and the Netherlands, as the differences reflect different assessment cultures and may shed light on some of the results of international large-scale assessments such as PISA.
Automatic inspection and analysis of digital waveform images by means of convolutional neural networks
Authors: Pignatelli Alessandro , D’Ajello Caracciolo Francesca , Console Rodolfo
Published: October 2021

Analyzing seismic data to get information about earthquakes has always been a major task for seismologists and, more in general, for geophysicists. Recently, thanks to the technological development of observation systems, more and more data are available to perform such tasks. However, this data “grow up” makes “human possibility” of data processing more complex in terms of required efforts and time demanding. That is why new technological approaches such as artificial intelligence are becoming very popular and more and more exploited. In this paper, we explore the possibility of interpreting seismic waveform segments by means of pre-trained deep learning. More specifically, we apply convolutional networks to seismological waveforms recorded at local or regional distances without any pre-elaboration or filtering. We show that such an approach can be very successful in determining if an earthquake is “included” in the seismic wave image and in estimating the distance between the earthquake epicenter and the recording station.
A scoping review of studies on the health impact of electronic nicotine delivery systems
Authors: Hajat Cother , Stein Emma , Shantikumar Saran , Niaura Raymond , Ferrara Pietro , Polosa Riccardo
Published: October 2021

We conducted a scoping review of studies on health outcomes from electronic nicotine delivery systems (ENDS). The objective was to identify, narratively synthesize, assess the strength and quality of evidence and critically appraise studies that have reported disease end points associated with the use of ENDS. We included published literature on the health impact of ENDS from 01/01/2015 until 01/02/2020 following the PRISMA guidelines using PubMed, Embase, Scopus and Google Scholar. The database search identified 755 studies, and other sources 265; 37 studies met final eligibility criteria. Levels of evidence included 24(65%) cross-sectional, one (2.7%) case–control and six (16%) case studies, four (11%) cohort studies, one (2.7%) randomized controlled trial (RCT) and one (2.7%) meta-analysis; 27(73%) studies reported only on harms, eight (22%) reported on benefits, two (2%) on benefits and harms. Quality ratings were poor in 20 (54%), fair in 9(24%) and good in 8(22%) of studies. In our review, ENDS was not shown to be causative for harmful cardiovascular disease (CVD) outcomes and shown to be beneficial for hypertensive patients. Switching from cigarettes to e-cigarettes resulted in reduced exacerbations of chronic obstructive pulmonary disease (COPD), with no evidence of long-term deterioration in lung function. Mental Health, cancer and mortality were not adequately studied to form any consensus. Our review has not demonstrated ENDS to be causative of harmful CVD outcomes; furthermore switching from cigarettes to e-cigarettes was associated with improved hypertensive control and reduced exacerbations of COPD, with no evidence of increased asthma risk or long-term respiratory harm. Mental health, cancer and mortality outcomes have not been adequately studied to form a conclusion. Overall, the findings of our review did not provide evidence to counter the consensus held by many that ENDS use is safer than the risks posed from smoking cigarettes.
PaO2/FiO2 ratio forecasts COVID-19 patients’ outcome regardless of age: a cross-sectional, monocentric study
Authors: Sinatti Gaia , Santini Silvano Junior , Tarantino Giovanni , Picchi Giovanna , Cosimini Benedetta , Ranfone Francesca , Casano Nicolò , Zingaropoli Maria Antonella , Iapadre Nerio , Bianconi Simone , Armiento Antonietta , Carducci Paolo , Ciardi Maria Rosa , Mastroianni Claudio Maria , Grimaldi Alessandro , Balsano Clara
Published: October 2021

We studied the predictive value of the PaO2/FiO2 ratio for classifying COVID-19-positive patients who will develop severe clinical outcomes. One hundred fifty patients were recruited and categorized into two distinct populations (“A” and “B”), according to the indications given by the World Health Organization. Patients belonging the population “A” presented with mild disease not requiring oxygen support, whereas population “B” presented with a severe disease needing oxygen support. The AUC curve of PaO2/FiO2 in the discovery cohort was 0.838 (95% CI 0.771–0.908). The optimal cut-off value for distinguishing population “A” from the “B” one, calculated by Youden’s index, with sensitivity of 71.79% and specificity 85.25%, LR+4.866, LR−0.339, was < 274 mmHg. The AUC in the validation cohort of 170 patients overlapped the previous one, i.e., 0.826 (95% CI 0.760–0.891). PaO2/FiO2 ratio < 274 mmHg was a good predictive index test to forecast the development of a severe respiratory failure in SARS-CoV-2-infected patients. Moreover, our work highlights that PaO2/FiO2 ratio, compared to inflammatory scores (hs-CRP, NLR, PLR and LDH) indicated to be useful in clinical managements, results to be the most reliable parameter to identify patients who require closer respiratory monitoring and more aggressive supportive therapies. Clinical trial registration: Prognostic Score in COVID-19, prot. NCT04780373 https://clinicaltrials.gov/ct2/show/NCT04780373 (retrospectively registered).
Comparison of characteristics and outcomes of patients admitted to hospital with COVID-19 during wave 1 and wave 2 of the current pandemic
Authors: Fluck David , Rankin Suzanne , Lewis Andrea , Robin Jonathan , Rees Jacqui , Finch Jo , Jones Yvonne , Jones Gareth , Kelly Kevin , Murray Paul , Wood Michael , Fry Christopher Henry , Han Thang Sieu
Published: October 2021

In this study of patients admitted with COVID-19, we examined differences between the two waves in patient characteristics and outcomes. Data were collected from the first COVID-19 admission to the end of study (01/03/2020–31/03/2021). Data were adjusted for age and sex and presented as odds ratios (OR) with 95% confidence intervals (CI). Among 12,471 admissions, 1452 (11.6%) patients were diagnosed with COVID-19. On admission, the mean (± SD) age of patients with other causes was 68.3 years (± 19.8) and those with COVID-19 in wave 1 was 69.4 years (± 18.0) and wave 2 was 66.2 years (± 18.4). Corresponding ages at discharge were 67.5 years (± 19.7), 63.9 years (± 18.0) and 62.4 years (± 18.0). The highest proportion of total admissions was among the oldest group (≥ 80 years) in wave 1 (35.0%). When compared with patients admitted with other causes, those admitted with COVID-19 in wave 1 and in wave 2 were more frequent in the 40–59 year band: 20.8, 24.6 and 30.0%; consisted of more male patients: 47.5, 57.6 and 58.8%; and a high LACE (Length of stay, Acuity of admission, Comorbidity and Emergency department visits) index (score ≥ 10): 39.4, 61.3 and 50.3%. Compared to wave-2 patients, those admitted in wave 1 had greater risk of death in hospital: OR = 1.58 (1.18–2.12) and within 30 days of discharge: OR = 2.91 (1.40–6.04). Survivors of COVID-19 in wave 1 stayed longer in hospital (median = 6.5 days; interquartile range = 2.9–12.0) as compared to survivors from wave 2 (4.5 days; interquartile range = 1.9–8.7). Patient characteristics differed significantly between the two waves of COVID-19 pandemic. There was an improvement in outcomes in wave 2, including shorter length of stay in hospital and reduction of mortality.
Response to BNT162b2 mRNA COVID-19 vaccine among healthcare workers in Italy: a 3-month follow-up
Authors: Ponticelli Domenico , Madotto Fabiana , Conti Sara , Antonazzo Ippazio C. , Vitale Andrea , Della Ragione Giovanni , Romano Maria L. , Borrelli Mario , Schiavone Beniamino , Polosa Riccardo , Ferrara Pietro , Mantovani Lorenzo G.
Published: October 2021

This study investigated the response to BNT162b2 mRNA COVID-19 vaccine among healthcare workers (HCWs) in an Italian teaching hospital. 444 participants were surveyed with either multiple RT-PCR assays for detection of SARS-CoV-2 nucleic acid in nasopharyngeal swabs or serology testing for the research of virus-specific immunoglobulins. Adverse events following immunization (AEFI) were reported. Two weeks after the first dose anti-SARS-CoV-2 antibodies exceeded reactivity cut-off in 82.5% the participants. Four HCWs tested positive at nasopharyngeal swab after 3 months. More than three-quarters reported AEFIs. Our findings offer an insight regarding the vaccine response after 3 months from its administration, with a special focus on effectiveness data, as well as the type and number of AEFIs complained by HCW recipients. The presented study may serve as reference for future research which will be necessary to explore the long-term safety of this vaccine, especially in population at high risk for infection, such as HCWs.
Global controversy in oyster systematics and a newly described species from SE Asia (Bivalvia: Ostreidae: Crassostreinae)
Authors: Sigwart Julia D. , Wong Nur Leena W. S. , Esa Yuzine
Published: October 2021

The diversity of native oysters in many regions is overshadowed by the global dominance of a few economically important species. Here we describe the Muar Oyster, Crassostrea (Magallana) saidii sp. nov., first reported as an established local fishery renowned for exceptional and distinctive flavour over 160 years ago by British colonial officials in Malaysia, but as yet never formally named or described as a species. This new species has a subtle but clear morphological diagnosis dependent on three-dimensional characters, which has long been recognised by local fishers to differentiate the new species from co-occurring C. (M.) belcheri (G. B. Sowerby II, 1871). The Indo-Pacific clade Magallana Salvi & Mariottini, 2016 in Salvi and Mariottini 2017 is a phylogenetically distinct group that nonetheless cannot be morphologically separated from the broader genus Crassostrea Sacco, 1897. Fossils or species known only from shell specimens, though morphologically distinct species, cannot be classified as Magallana, Talonostrea Li & Qi, 1994, or Crassostrea s.s.; therefore, we revise these groups as sub-genera within Crassostrea. Our analysis of the COI barcoding fragment from previously published sequences of all available Magallana species found that gene is not sufficient to separate several economically important species, and revealed more than 5% of sequences in GenBank represent identification errors. The new species Crassostrea (Magallana) saidii sp. nov., which is genetically, morphologically, and gastronomically distinct, is known from only one population under potential threat from urbanisation. Many more global species of Magallana remain undescribed. The systematics of this group is critical to understand the diversity of global oysters, and to understand the sustainable use of species grown worldwide for centuries as our food.
Cardiac sarcomere mechanics in health and disease
Authors: Crocini Claudia , Gotthardt Michael
Published: October 2021

The sarcomere is the fundamental structural and functional unit of striated muscle and is directly responsible for most of its mechanical properties. The sarcomere generates active or contractile forces and determines the passive or elastic properties of striated muscle. In the heart, mutations in sarcomeric proteins are responsible for the majority of genetically inherited cardiomyopathies. Here, we review the major determinants of cardiac sarcomere mechanics including the key structural components that contribute to active and passive tension. We dissect the molecular and structural basis of active force generation, including sarcomere composition, structure, activation, and relaxation. We then explore the giant sarcomere-resident protein titin, the major contributor to cardiac passive tension. We discuss sarcomere dynamics exemplified by the regulation of titin-based stiffness and the titin life cycle. Finally, we provide an overview of therapeutic strategies that target the sarcomere to improve cardiac contraction and filling.
Stigma in Psychiatry: Impact of a Virtual and Traditional Psychiatry Clerkship on Medical Student Attitudes
Authors: Bazaid Khalid , Simas Kevin , Bezzahou Abdellah
Published: October 2021

Objective

The objective of the study was to assess the change in medical students’ attitudes towards psychiatry following a virtual clerkship experience compared to a traditional clerkship experience.

Method

Ninety-seven medical students from the University of Ottawa were assessed pre- and post-clerkship on the ATP-30 (Attitudes Towards Psychiatry-30) measure. Cohorts of students were categorized as pre-COVID or during-COVID depending on when and how they experienced their clerkship (traditional or virtual). The total student response rate was approximately 48%. A quasi-experimental design was implemented, and non-parametric statistics were used to analyze the data.

Results

Medical students’ overall attitudes towards psychiatry improved from pre- to post-clerkship, with the type of clerkship experience (traditional or virtual) having no significant impact on the magnitude to which attitudes improved.

Conclusion

Implementation of a virtual clerkship in psychiatry did not deteriorate medical student attitudes towards psychiatry as a specialty, with both the traditional and virtual clerkship program enhancing students’ attitudes towards psychiatry favorably.
Digital twins of the mooring line tension for floating offshore wind turbines to improve monitoring, lifespan, and safety
Authors: Walker Jake , Coraddu Andrea , Collu Maurizio , Oneto Luca
Published: October 2021

The number of installed floating offshore wind turbines (FOWTs) has doubled since 2017, quadrupling the total installed capacity, and is expected to increase significantly over the next decade. Consequently, there is a growing consideration towards the main challenges for FOWT projects: monitoring the system’s integrity, extending the lifespan of the components, and maintaining FOWTs safely at scale. Effectively and efficiently addressing these challenges would unlock the wide-scale deployment of FOWTs. In this work, we focus on one of the most critical components of the FOWTs, the Mooring Lines (MoLs), which are responsible for fixing the structure to the seabed. The primary mechanical failure mechanisms in MoLs are extreme load and fatigue, both of which are functions of the axial tension. An effective solution to detect long-term drifts in the mechanical response of the MoLs is to develop a Digital Twin (DT) able to accurately predict the behaviour of the healthy system to compare with the actual one. Moreover, we will develop another DT able to accurately predict the near future axial tension as an effective tool to improve the lifespan of the MoLs and the safety of FOWT maintenance operations. In fact, by changing the FOWT operational settings, according to the DT prediction, operators can increase the lifespan of the MoLs by reducing the stress and, additionally, in the case where FOWT operational maintenance is in progress, the prediction from the DT can serve as early safety warning to operators. Authors will leverage operational data collected from the world’s first commercial floating-wind farm [the Hywind Pilot Park (https://www.equinor.com/en/what-we-do/floating-wind/hywind-scotland.html.)] in 2018, to investigate the effectiveness of DTs for the prediction of the MoL axial tension for the two scenarios depicted above. The DTs will be developed using state-of-the-art data-driven methods, and results based on real operational data will support our proposal.
Responses to the Uyghur Crisis and the Implications for Business and Human Rights Legislation
Authors: POLASCHEK Rosa
Published: October 2021

As has now been well publicized, there is serious and credible evidence that Uyghur and other minority communities in China are being forced into internment or ‘re-education’ camps,1 with strong links to subsequent forced labour in factories, particularly centred in Xinjiang province.2 The use of forced labour (intimately connected to many international supply chains) as a hallmark feature of the Chinese state’s oppression of its Uyghur peoples requires a ‘business and human rights’ (BHR) lens to responses to the human rights violations in the region.
Adaptation of a school-based mental health literacy curriculum: from Canadian to English classrooms
Authors: Mansfield Rosie , Humphrey Neil , Patalay Praveetha , Moore Anna , Stapley Emily
Published: October 2021

Background

School-based mental health literacy (MHL) interventions are increasingly trialled outside of the country in which they were developed. However, there is a lack of published studies that qualitatively explore their cultural adaptation. This study investigated the reasons for adaptations made and suggested to a Canadian MHL curriculum (The Guide) within the English school context.

Method

Semi-structured interviews were conducted with 11 school staff responsible for the planning and/or implementation of The Guide across three schools in the South East of England, as part of the Education for Wellbeing (EfW) feasibility study. Transcripts were analysed using a hybrid, deductive-inductive thematic analysis.

Results

Adaptations made and suggested included dropping and emphasising content, and adapting language, examples and references. Most adaptations were proactive and related to The Guide's implementation methods, including developing more interactive and student-led approaches.Staff Capacity and Expertise,Timetabling,andAccessibility of Resourceswere identified as logistical reasons for adaptations. Philosophical reasons includedConsistency of Messages,Student Characteristics,Reducing Stigma and Empowering Students,National and Local Context,andAppropriate Pedagogic Practices.

Conclusion

Overall, recommendations were for immediately implementable lesson plans informed by teachers' knowledge about best pedagogic practices in England. Adequate training, attended by both senior leadership and those implementing, was also emphasised. While ensuring that the core components are clear, MHL interventions should be developed with a necessary level of flexibility to accommodate contextual characteristics. Future research should ensure that adaptations are captured through process and implementation evaluations conducted alongside efficacy trials.
Affective Polarization and the Populist Radical Right: Creating the Hating?
Authors: Harteveld Eelco , Mendoza Philipp , Rooduijn Matthijs
Published: October 2021

Do populist radical right (PRR) parties fuel affective polarization? If so, how and under which circumstances? Based on a comparative cross-country analysis covering 103 elections in 28 European countries and an examination of longitudinal data from the Netherlands, we show that PRR parties occupy a particular position in the affective political landscape because they both radiate and receive high levels of dislike. In other words, supporters of PRR parties are uniquely (and homogeneously) negative about (supporters of) mainstream parties and vice versa. Our analyses suggest that these high levels of antipathy are most likely due to the combination of these parties' nativism and populism – two different forms of ingroup–outgroup thinking. Our findings also suggest that greater electoral success by PRR parties reduces dislike towards them, while government participation appears threatening to all voters except coalition partners.
Arrived in Power, and Yet Still Disgruntled? How Government Inclusion Moderates ‘Protest Voting’ for Far-Right Populist Parties in Europe
Authors: Muis Jasper , Brils Tobias , Gaidytė Teodora
Published: October 2021

While debates about far-right populism often concentrate on Central and Eastern Europe, research on these parties predominantly focuses on Western countries. Addressing this remarkable gap, this article revisits the ‘protest voting’ explanation for electoral support for the far right. Using European Social Survey data (2002–16) from 22 countries, we show that political dissatisfaction is a stronger explanatory factor when far-right parties are in opposition, but is a less important determinant of electoral support when they are in government. Previous findings based on Western Europe – which similarly showed that the anti-elite hypothesis is less relevant when far-right parties join government coalitions – travel well to post-communist European countries. In Hungary and Poland, we even find that far-right voters have become less distrustful of national political institutions than the rest of the electorate. Our conclusion implies that anti-elite populism is context-dependent and has limited use for understanding successes of leaders such as Wilders, Salvini and Orbán.
When Is A Pledge A Pledge?
Authors: Krishnarajan Suthan , Jensen Carsten
Published: October 2021

Despite the central role of election pledges in modern representative democracy, it remains uncertain how voters define pledges. We examine this by focusing on four rhetorical dimensions of political statements: the pledge giver, the formulation of commitment, the policy content and quantification. In three conjoint experiments on representative samples totalling around 6,000 respondents in the United States, Britain and Denmark, we find remarkably consistent results. On the one hand, voters consistently differentiate between statements in a highly focused manner: a promise is a promise if it is sincere and realistic – no matter who made it and whether it can be checked. On the other hand, voters are not willing to hold their party accountable for a given statement – even if they consider it an election pledge. We demonstrate that this is the perceptual logic of election pledges in Western democracies.
Opportunity cost determines free-operant action initiation latency and predicts apathy
Authors: Nair Akshay , Niyogi Ritwik K. , Shang Fei , Tabrizi Sarah J. , Rees Geraint , Rutledge Robb B.
Published: October 2021

Background

Apathy, a disabling and poorly understood neuropsychiatric symptom, is characterised by impaired self-initiated behaviour. It has been hypothesised that the opportunity cost of time (OCT) may be a key computational variable linking self-initiated behaviour with motivational status. OCT represents the amount of reward which is foregone per second if no action is taken. Using a novel behavioural task and computational modelling, we investigated the relationship between OCT, self-initiation and apathy. We predicted that higher OCT would engender shorter action latencies, and that individuals with greater sensitivity to OCT would have higher behavioural apathy.

Methods

We modulated the OCT in a novel task called the ‘Fisherman Game’, Participants freely chose when to self-initiate actions to either collect rewards, or on occasion, to complete non-rewarding actions. We measured the relationship between action latencies, OCT and apathy for each participant across two independent non-clinical studies, one under laboratory conditions (n = 21) and one online (n = 90). ‘Average-reward’ reinforcement learning was used to model our data. We replicated our findings across both studies.

Results

We show that the latency of self-initiation is driven by changes in the OCT. Furthermore, we demonstrate, for the first time, that participants with higher apathy showed greater sensitivity to changes in OCT in younger adults. Our model shows that apathetic individuals experienced greatest change in subjective OCT during our task as a consequence of being more sensitive to rewards.

Conclusions

Our results suggest that OCT is an important variable for determining free-operant action initiation and understanding apathy.
Evidence that a novel transdiagnostic eating disorder treatment reduces reward region response to the thin beauty ideal and high-calorie binge foods
Authors: Stice Eric , Yokum Sonja , Rohde Paul , Gau Jeff , Shaw Heather
Published: October 2021

Background

Findings from brain imaging studies with small samples can show limited reproducibility. Thus, we tested whether the evidence that a transdiagnostic eating disorder treatment reduces responsivity of brain valuation regions to thin models and high-calorie binge foods, the intervention targets, from a smaller earlier trial emerged when we recruited additional participants.

Methods

Women with DSM-5 eating disorders (N = 138) were randomized to the dissonance-based body project treatment (BPT) or a waitlist control condition and completed functional magnetic resonance imaging (fMRI) scans assessing neural response to thin models and high-calorie foods at pretest and posttest.

Results

BPT v. control participants showed significantly greater reductions in responsivity of regions implicated in reward valuation (caudate) and attentional motivation (precuneus) to thin v. average-weight models, echoing findings from the smaller sample. Data from this larger sample also provided novel evidence that BPT v. control participants showed greater reductions in responsivity of regions implicated in reward valuation (ventrolateral prefrontal cortex) and food craving (hippocampus) to high-calorie binge foods v. low-calorie foods, as well as significantly greater reductions in eating disorder symptoms, abstinence from binge eating and purging behaviors, palatability ratings for high calorie foods, monetary value for high-calorie binge foods, and significantly greater increases in attractiveness ratings of average weight models.

Conclusions

Results from this larger sample provide evidence that BPT reduces valuation of the thin ideal and high-calorie binge foods, the intervention targets, per objective brain imaging data, and produces clinically meaningful reductions in eating pathology.
Dynamics of diurnal cortisol and alpha-amylase secretion and their associations with PTSD onset in recent interpersonal trauma survivors
Authors: Kinney Kerry L. , Rao Uma , Bailey Brooklynn , Hellman Natalie , Kelly Chris , McAfee Nicholas W. , Morris Matthew C.
Published: October 2021

Background

Dysfunction in major stress response systems during the acute aftermath of trauma may contribute to risk for developing posttraumatic stress disorder (PTSD). The current study investigated how PTSD diagnosis and symptom severity, depressive symptoms, and childhood trauma uniquely relate to diurnal neuroendocrine secretion (cortisol and alpha-amylase rhythms) in women who recently experienced interpersonal trauma compared to non-traumatized controls (NTCs).

Method

Using a longitudinal design, we examined diurnal cortisol and alpha-amylase rhythms in 98 young women (n= 57 exposed to recent interpersonal trauma,n= 41 NTCs). Participants provided saliva samples and completed symptom measures at baseline and 1-, 3-, and 6-month follow-up.

Results

Multilevel models (MLMs) revealed lower waking cortisol predicted the development of PTSD in trauma survivors and distinguished at-risk women from NTCs. Women with greater childhood trauma exposure exhibited flatter diurnal cortisol slopes. Among trauma-exposed individuals, lower waking cortisol levels were associated with higher concurrent PTSD symptom severity. Regarding alpha-amylase, MLMs revealed women with greater childhood trauma exposure exhibited higher waking alpha-amylase and slower diurnal alpha-amylase increase.

Conclusions

Results suggest lower waking cortisol in the acute aftermath of trauma may be implicated in PTSD onset and maintenance. Findings also suggest childhood trauma may predict a different pattern of dysfunction in stress response systems following subsequent trauma exposure than the stress system dynamics associated with PTSD risk; childhood trauma appears to be associated with flattened diurnal cortisol and alpha-amylase slopes, as well as higher waking alpha-amylase.
Disclosure versus recognition: the value relevance of pensions
Authors: Larcher Luca
Published: October 2021

This paper compares how pension obligations impact the market value of United States corporations under two accounting regimes. Using a sample of firms that disclosed pension liabilities under Statement of Financial Accounting Standards (SFAS) No. 87 from 2001 to 2005 and recognized them under SFAS No. 158 from 2006 to 2014, I find that equity market participants take into account the net position of the pension fund only if it is recognized on the sponsor's balance sheet, thus mispricing the pension deficit/surplus under the disclosure regime. I also provide evidence suggesting that investors' perception of pension deficits/surpluses changed with the introduction of SFAS No. 158 in 2006.
One country, two systems: evidence on retirement patterns in China
Authors: Giles John , Lei Xiaoyan , Wang Gewei , Wang Yafeng , Zhao Yaohui
Published: October 2021

This paper documents the patterns and correlates of retirement in China using a nationally representative survey, the China Health and Retirement Longitudinal Study. After documenting stark differences in retirement ages between urban and rural residents, the paper shows that China's urban residents retire earlier than workers in many Organization for Economic Co-operation and Development countries and that rural residents continue to work until advanced ages. Differences in access to generous pensions and economic resources explain much of the urban–rural difference in retirement rates. The paper suggests that reducing disincentives created by China's Urban Employee Pension system, improving health status, providing childcare and elder care support may all facilitate longer working lives. Given spouse preferences for joint retirement, creating incentives for women to retire later may facilitate longer working lives for both men and women.