bluuebunny/crossref_metadata_2025 huggingface dataset:
Pressure Pain Hyperalgesia Expressed by Topographical Pressure Pain Sensitivity after Cardiac Surgery
Authors: Segura-Méndez Bárbara , Planchuelo-Gómez Álvaro , Fuentes-Martín Álvaro , Madeleine Pascal , Guerrero Ángel L. , Carrascal Yolanda , Fernández-de-las-Peñas César
Published: September 2024

Backgrounds: We aim to evaluate changes in pressure pain sensitivity before and after cardiac surgery using topographical sensitivity maps utilizing a pressure algometer. Methods: Pressure pain thresholds over 17 thoracic points and 4 distant pain-free points were assessed in 70 patients (women: 29, age: 67.5 years), before and at 1, 3, and 7 postoperative days. Thoracic topographical pressure pain sensitivity maps were calculated at all follow-ups. Postoperative pain was recorded at each follow-up on a numerical pain rate scale. Results: Postoperative pain intensity decreased from 6.4 (SD 1.0) on the first postoperative day to 5.5 (SD 1.9) on the third and to 4.5 (SD 1.7) on the seventh day (p < 0.001). The mixed-model ANOVA revealed that the lowest pressure pain thresholds were observed one day after surgery, increased slightly during follow-up, and were lower at the xiphoid process. Significant negative correlations between postoperative pain intensity and pressure pain thresholds were observed at each time point in thoracic measures (all, p < 0.01), but not with pressure pain thresholds from distant pain-free areas. Conclusions: Postoperative pain after cardiac surgery can be objectively quantified using algometry. Pressure pain hyperalgesia was associated with the intensity of postoperative pain.
Lipid Profile of Fresh and Aged Wollemia nobilis Seeds: Omega-3 Epoxylipid in Older Stored Seeds
Authors: Ng Michelle C. H. , Tran Van Hoan , Duke Rujee Kyokajee , Offord Catherine A. , Meagher Patricia F. , Cui Pei Hong , Duke Colin Charles
Published: September 2024

Wollemi pine, Wollemia nobilis W. G. Jones, K. D. Hill & J. M. Allan (Araucariaceae) was discovered in a remote canyon 150 km north-west of Sydney, Australia. As fewer than 100 adult trees of this plant survive in the wild, efforts to conserve this species have included seed storage. Fresh and stored seeds were analysed for yield and composition of the seed oil. The seed kernels, from both fresh and stored seed, were rich in oil with contents of 42% and 48%, respectively. The fatty acid profile of Wollemi pine seed oil was determined by GC-MS analyses of fatty acid methyl ester derivatives. Oleic acid makes up 32% of the fatty acid profile, while the major polyunsaturated fatty acid is linoleic acid (25%). Most of the detectable omega-3 fatty acid content of the oil is α-linolenic acid (3%). The seed oil has a high content of C20 to C24 fatty acids (25%) consisting of long-chain saturated fatty acids (19%). The polyunsaturated C20 omega-6 fatty acid content consists of eicosadienoic acid, dihomo-γ-linolenic acid, and arachidonic acid (total 4%). 1H NMR analyses of the intact oil showed that the lipids were largely in the form of triglycerides with a degree of unsaturation of 1.5 double bond equivalents per fatty acid residue. In artificially aged or stored seeds, minor additional 1H NMR spectral signals were attributed to an omega-3 epoxylipid, tentatively identified as cis-15,16-epoxy-9Z,12Z-octadecadienoic acid or ester derivative. Other minor signals were characteristic of a hydroxy or a hydroperoxy E,Z diene containing fatty acid. These products are typically formed by metabolic lipid oxidation of fatty acids. The content of the omega-3 epoxylipid, determined by the 1H NMR method, varied with storage conditions and duration from less than 0.1% to a maximum of 3.3%.
Synchronization of Chaotic Extremum-Coded Random Number Generators and Its Application to Segmented Image Encryption
Authors: Araki Shunsuke , Wu Ji-Han , Yan Jun-Juh
Published: September 2024

This paper proposes a highly secure image encryption technique based on chaotic synchronization. Firstly, through the design of a synchronization controller, we ensure that the master–slave chaotic extremum-coded random number generators (ECRNGs) embedded in separated transmitters and receivers are fully synchronized to provide synchronized dynamic random sequences for image encryption applications. Next, combining these synchronized chaotic sequences with the AES encryption algorithm, we propose an image segmentation and multi-encryption method to enhance the security of encrypted images and realize a secure image transmission system. Notably, in the design of the synchronization controller, the transient time before complete synchronization between the master and slave ECRNGs is effectively controlled by specifying the eigenvalues of the matrix in the synchronization error dynamics. Research results in this paper also show that complete synchronization of ECRNGs can be achieved within a single sampling time, which significantly contributes to the time efficiency of the image transmission system. As for the image encryption technique, we propose the method of image segmentation and use the synchronized dynamic random sequences generated by the ECRNGs to produce the keys and initialization vectors (IVs) required for AES-CBC image encryption, greatly enhancing the security of the encrypted images. To highlight the contribution of the proposed segmented image encryption, statistical analyses are conducted on the encrypted images, including histogram analysis (HA), information entropy (IE), correlation coefficient analysis (CCA), number of pixels change rate (NPCR), and unified average changing intensity (UACI), and compared with existing literature. The comparative results fully demonstrate that the proposed encryption method significantly enhances image encryption performance. Finally, under the network transmission control protocol (TCP), the synchronization of ECRNGs, dynamic keys, and IVs is implemented as well as segmented image encryption and transmission, and a highly secure image transmission system is realized to validate the practicality and feasibility of our design.
Prediction of Turbulent Boundary Layer Flow Dynamics with Transformers
Authors: Sarma Rakesh , Hübenthal Fabian , Inanc Eray , Lintermann Andreas
Published: September 2024

Time-marching of turbulent flow fields is computationally expensive using traditional Computational Fluid Dynamics (CFD) solvers. Machine Learning (ML) techniques can be used as an acceleration strategy to offload a few time-marching steps of a CFD solver. In this study, the Transformer (TR) architecture, which has been widely used in the Natural Language Processing (NLP) community for prediction and generative tasks, is utilized to predict future velocity flow fields in an actuated Turbulent Boundary Layer (TBL) flow. A unique data pre-processing step is proposed to reduce the dimensionality of the velocity fields, allowing the processing of full velocity fields of the actuated TBL flow while taking advantage of distributed training in a High Performance Computing (HPC) environment. The trained model is tested at various prediction times using the Dynamic Mode Decomposition (DMD) method. It is found that under five future prediction time steps with the TR, the model is able to achieve a relative Frobenius norm error of less than 5%, compared to fields predicted with a Large Eddy Simulation (LES). Finally, a computational study shows that the TR achieves a significant speed-up, offering computational savings approximately 53 times greater than those of the baseline LES solver. This study demonstrates one of the first applications of TRs on actuated TBL flow intended towards reducing the computational effort of time-marching. The application of this model is envisioned in a coupled manner with the LES solver to provide few time-marching steps, which will accelerate the overall computational process.
Instability of Standing Waves for INLS with Inverse Square Potential
Authors: Almuthaybiri Saleh , Saanouni Tarek
Published: September 2024

This work studies an inhomogeneous generalized Hartree equation with inverse square potential. The purpose is to prove the existence and strong instability of inter-critical standing waves. This means that there are infinitely many data near to the ground state, such that the associated solution blows-up in finite time. The proof combines a variational analysis with the standard variance identity. The challenge is to deal with three difficulties: the singular potential |x|−2, an inhomogeneous term |x|−λ, and a non-local source term.
Management of Coronary Artery Diseases in Systemic Vasculitides: Complications and Strategies
Authors: Shumnalieva Russka , Mileva Niya , Padjen Ivan , Siliogkas Periklis , Chervenkov Lyubomir , Bakopoulou Konstantina , Kaouri Issa El , Vasilska Anna , Miteva Dimitrina , Vassilev Dobrin , Velikova Tsvetelina
Published: September 2024

Coronary artery disease (CAD) presents a significant risk for patients with systemic vasculitides, a group of disorders characterized by the inflammation of blood vessels. In this review, we focus on the pathophysiological mechanisms, complications, and management strategies for CAD in systemic vasculitides. We highlight how the inflammatory processes inherent in vasculitis contribute to accelerated atherosclerosis and myocardial ischemia. Key strategies in managing CAD in this patient population include using medicine treatments to mitigate vascular inflammation while balancing the risk of promoting cardiovascular events and lifestyle modifications. Understanding the nuanced relationship between systemic vasculitides and CAD is crucial for improving patient outcomes and guiding therapeutic approaches.
Isolated Severe Neutropenia in Adults, Evaluation of Underlying Causes and Outcomes, Real-World Data Collected over a 5-Year Period in a Tertiary Referral Hospital
Authors: Njue Linet , Porret Naomi , Schnegg-Kaufmann Annatina Sarah , Varra Luca Francesco , Andres Martin , Rovó Alicia
Published: September 2024

Background and Objectives: In clinical practice, neutropenia is frequently accompanied by other cytopenia; isolated non-chemotherapy-induced severe neutropenia is less frequent and its differential diagnosis can be challenging. In this real-world study with data collected over a 5-year period in a tertiary referral hospital, we primarily sought to identify underlying causes of isolated severe neutropenia (<0.5 × 109/L). Secondly, we aimed to analyze its management and outcomes. Materials and Methods: From 444,926 screened patients, after exclusion of patients with chemotherapy, radiotherapy, hematological neoplasms, additional cytopenia, and benign ethnic neutropenia, we identified and analyzed data from 70 patients (0.015%) with isolated severe neutropenia. We thus confirmed that the occurrence of isolated severe neutropenia is a rare event, even in a tertiary hospital. Results: The median age at diagnosis was 34 years (range 1–81) and 65% were female. Acute neutropenia was more frequently observed (n = 46/70, 65.7%); the main underlying causes in this group were drugs (n = 36/46, 78%) followed by infections (n = 10/46, 21.7%). We identified 24 (34.3%) patients with chronic neutropenia. The majority of them (n = 12/24, 50%) had an idiopathic form (CIN), 8/24 (33%) were autoimmune (AIN), and 4/24 (17%) were congenital. Conclusions: This study demonstrates the rarity and heterogeneity of isolated severe neutropenia and the steps to consider in its diagnostic work-up and management. Epidemiological characteristics, diagnostic work-up, and management including hospitalizations are described. Due to the high frequency of metamizole-induced neutropenia observed in this study, we want to raise awareness about its use, since this complication generates frequent hospitalizations even in young, otherwise healthy patients. Furthermore, recurrent infections in chronic forms of idiopathic neutropenia were quite common, suggesting a difference in phenotypes and need for therapy consideration depending on the clinical course.
Molecular Characterization of Carbapenem and Colistin Resistance in Klebsiella pneumoniae Isolates Obtained from Clinical Samples at a University Hospital Center in Algeria
Authors: Rihane Riyane , Hecini-Hannachi Abla , Bentchouala Chafia , Benlabed Kaddour , Diene Seydina M.
Published: September 2024

The current study aimed to determine the molecular mechanisms of carbapenem and colistin resistance among the clinical isolates of Klebsiella pneumoniae from hospitalized patients admitted to a university hospital in Eastern Algeria. In total, 124 non-duplicate isolates of K. pneumoniae were collected from September 2018 to April 2019. Bacterial identification was performed using MALDI-TOF MS. The presence of extended spectrum β-lactamase (ESBL) genes, carbapenemase genes, chromosomal mutation and mcr genes in colistin-resistant K. pneumoniae were evaluated by PCR. ESBLs represented a rate of 49.1% and harbored blaCTX-M, blaTEM and blaSHV genes. Concerning carbapenems, 12 strains (9.6%) were resistant to ertapenem (MIC: 1–32 μg/mL), of which one strain (0.8%) was also resistant to imipenem (MIC: 32 μg/mL). Among these strains, nine (75%) harbored blaOXA-48 gene. Seven strains (5.6%) expressed resistance to colistin (MIC: 2–32 μg/mL), of which two harbored mcr-8 and mgrB genes simultaneously. The existence of a double resistance to colistin in the same strain is new in Algeria, and this could raise concerns about the increase in levels of resistance to this antibiotic (MIC: 32 μg/mL). The mgrB gene alone was observed in five isolates (71.4%), including two strains harboring blaOXA-48. This is the first report revealing the presence of K. pneumoniae strains carrying the blaOXA-48 gene as well as a mutation in the mgrB gene. Large-scale surveillance and effective infection control measures are also urgently needed to prevent the outbreak of various carbapenem- and colistin-resistant isolates.
Microbial Contributions to Heavy Metal Phytoremediation in Agricultural Soils: A Review
Authors: Khatoon Zobia , Orozco-Mosqueda Ma. del Carmen , Santoyo Gustavo
Published: September 2024

Phytoremediation is a sustainable technique that employs plants to reinforce polluted environments such as agroecosystems. In recent years, new strategies involving the plant microbiome as an adjuvant in remediation processes have been reported. By leveraging this microbial assistance to remediate soils contaminated with heavy metals such As, Pb, Cd, Hg, and Cr, plants can sequester, degrade, or stabilize contaminants more efficiently. Remarkably, some plant species are known for their hyper-accumulative traits in synergy with their microbial partners and can successfully mitigate heavy metal pollutants. This sustainable biotechnology based on plant–microbe associations not only aids in environmental cleanup but also enhances biodiversity, improves soil structure, and promotes plant growth and health, making it a promising solution for addressing agro-pollution challenges worldwide. The current review article emphasizes the potential of synergistic plant–microbe interactions in developing practical and sustainable solutions for heavy metal remediation in agricultural systems, which are essential for food security.
Characterization and Analysis of the Main Factors of Brittleness of Shale Oil Reservoirs in the Liushagang Formation, X Depression, Beibuwan Basin
Authors: Lai Fuqiang , Liu Yuejiao , Tang Mingzheng , Zeng Chengxiang , Wang Ruyue
Published: September 2024

The analysis of the main factors of brittleness is an important basis for the selection of engineering desserts in shale oil reservoirs. In this study, with the shale oil reservoir of the Liushagang Formation in the X Depression of the Beibuwan Basin as the research object, a characterization and analysis of the main factors of brittleness of the reservoir was performed in order to further reveal the brittleness of shale reservoirs in the study area. The brittleness of reservoirs in the study area was controlled by both internal and external factors, and the main factors of brittleness in the target section included the maturity of organic matter, horizontal stress difference, and brittle minerals. As the maturity of organic matter increased, the density, elastic modulus, and hardness of casein increased and the differentially hardened internal structure occurred and significantly affected brittleness. The mineral composition of the reservoir was characterized by complex mineral types and high contents of brittle minerals, and the minerals determining brittleness were mainly quartz, feldspar, calcite, and dolomite. The horizontal stress difference of the shale oil section was relatively small and contributed to fracturing and reforming. This study clarified the brittleness characteristics of E2l shale and its main factors, and provided a basis for the selection of shale formation geo-engineering dessert layers in the study area.
Origin and Evolution of Ore-Forming Fluid and Metallogenic Mechanism of the Baoshan Cu-Pb-Zn Deposit, South China: Constraints of Fluid Inclusion and C-H-O Isotopes
Authors: Dai Xueling , Li Yongshun , Zhang Junke , Liu Zhongfa , Chen Ke , He Mingpeng
Published: September 2024

The Southern Hunan area is located in the superposition of the Qin-Hang Cu-Pb-Zn polymetallic ore belt and the Nanling W-Sn-Mo polymetallic ore belt, which is an important window to study the mineralization of W-Sn-Mo and Cu-Pb-Zn polymetallic deposits. The Baoshan deposit is a large Cu-Pb-Zn polymetallic deposit in Southern Hunan Province with obvious zones of Cu mineralization and Pb-Zn mineralization: the central part of the Baoshan deposit demonstrates contact metasomatic (skarn) Cu mineralization, while the western, northern and eastern parts demonstrate hydrothermal vein Pb-Zn mineralization. However, the origin and evolution of the ore-forming fluid and mechanism of Cu and Pb-Zn mineral precipitation are still unclear. The metallogenic process of the Baoshan Cu-Pb-Zn deposit can be divided into four stages: (1) the early skarn stage (S1); (2) the late skarn stage (S2); (3) the Cu-Fe sulfide stage (S3); and (4) the Pb-Zn sulfide stage (S4). The results of microtemperature measurements and a Raman spectrometric analysis of fluid inclusions show that the ore-forming fluid was the H2O-NaCl (-CO2 ± N2 ± C2H6) system in the skarn stages (S1 + S2) and changed into the H2O-NaCl-CO2 (±N2 ± C2H6) system in the sulfide stages (S3 + S4). The temperature (S1: 436.6~548.2 °C; S2: 344.1~435.1 °C; S3: 134.1~413.1 °C; S4: 183.9~261.0 °C) and salinity (S1: 17.4~51.2 wt.%NaClequiv; S2: 13.6~41.7 wt.%NaClequiv; S3: 1.2~32.3 wt.%NaClequiv; S4: 1.8~9.6 wt.%NaClequiv) showed a downward trend from the early to late stages. From the skarn stages (S1 + S2) to the sulfide stages (S3 + S4), the ore-forming pressure results from the static rock pressure and the hydrostatic pressure, and the ore-forming depth is estimated to be about three to six km. The C-H-O isotopic compositions of hydrothermal minerals such as quartz and calcite indicate that the ore-forming fluid is predominately magmatic fluid, but a significant amount of meteoric water is added in the Pb-Zn sulfide stage (S4). The formation of the mineralization zonation of the Baoshan deposit is the result of many factors (e.g., stratigraphy, structure and metal precipitation mechanism): the Cu mineralization is controlled by the contact zone, and the Pb-Zn mineralization is controlled by the fault. In addition, the precipitation of Cu is mainly controlled by fluid boiling, while the precipitation of Pb and Zn is mainly controlled by the mixing of magmatic fluid and meteoric water.
Study on the Dynamic Process of the Attachment of a Single Bubble to Rough Surfaces with Different Hydrophobicity
Authors: Chen Songjiang , Wang Jiarui , Lei Gang , Ma Wanqi , Zhang Ningning , Yu Yuexian , Zhu Zhanglei , Li Zhen
Published: September 2024

A stable attachment between bubbles and solid particles is essential for flotation. Therefore, it is particularly necessary to study the dynamic process that occur in the attachment of bubbles to a solid surface. In this paper, Teflon and plexiglass plates were used as hydrophobic and hydrophilic solid surfaces, respectively, and solid surfaces with roughness of 0.018 μm to 5.33 μm were prepared by polishing with sandpaper. The influence of roughness on the dynamic process in bubble attachment to solid surfaces with different hydrophobicity was studied via a high-speed camera (750 frames per second). It was found that roughness played a positive role in the attachment to the hydrophobic Teflon surface while a negative role in the attachment to the hydrophilic plexiglass surface in terms of the bubble’s attachment to the solid plates. For a smooth Teflon surface, the formation of three–phase contact (i.e., the drainage of wetting film) took up to 95 ms, whereas for a very rough Teflon surface it took only 5 milliseconds. On the contrary, the high roughness prevented the bubble from attaching to the hydrophilic plexiglass surface. It was concluded that the increased roughness of Teflon plates was conductive to air entrapment in surface irregularities, inducing the rapid rupture of the wetting film on a very rough Teflon surface, while the increased roughness of the plexiglass plates resulted in “water pockets” in surface grooves, making the wetting film on the plexiglass surface stable.
Opal Synthesis: Toward Geologically Relevant Conditions
Authors: Gouzy Simon , Rondeau Benjamin , Vinogradoff Vassilissa , Chauviré Boris , Coulet Marie-Vanessa , Grauby Olivier , Terrisse Hélène , Carter John
Published: September 2024

Natural opal is a widespread mineral formed by the aqueous alteration of silicate rocks. It occurs as a mixture of silica nano-to-micro-structures (e.g., nanograins, spheres) and silica hydrogel cement, with variations in the proportions of these components leading to significant differences in the physico-chemical properties of opals. However, the detailed process of their formation in nature and the influence of the mixing ratio are not fully understood, as opal has not been yet synthesized under geologically relevant conditions. This study aims to develop a method of opal synthesis in conditions close to continental weathering conditions (<50 °C, ambient pressure) using relevant chemicals that could be employed to gain insight into the processes that give rise to opal on Earth and Mars. Our synthesis method enabled us to synthesize opal-A with different mixing ratios, of which four were then studied to determine the effect on the material’s properties. Changes in the proportion of the hydrogel cement affect the porosity and the total water content, as well as the proportion of “water” species (H2O and OH). Moreover, the synthetic opal obtained with a 1:1 ratio shows the closest similarity to natural opal-AG. Finally, our results support the hypothesized multistage process for opal formation in nature.
Nematicidal and Insecticidal Compounds from the Laurel Forest Endophytic Fungus Phyllosticta sp.
Authors: Díaz Carmen E. , Andrés María Fe , Bolaños Patricia , González-Coloma Azucena
Published: September 2024

The search for natural product-based biopesticides from endophytic fungi is an effective tool to find new solutions. In this study, we studied a pre-selected fungal endophyte, isolate YCC4, from the paleoendemism Persea indica, along with compounds present in the extract and the identification of the insect antifeedant and nematicidal ones. The endophyte YCC4 was identified as Phyllosticta sp. by molecular analysis. The insect antifeedant activity was tested by choice bioassays against Spodoptera littoralis, Myzus persicae, and Rhopalosiphum padi, and the in vitro and in vivo mortality was tested against the root-knot nematode Meloidogyne javanica. Since the extract was an effective insect antifeedant, a strong nematicidal, and lacked phytotoxicity on tomato plants, a comprehensive chemical study was carried out. Two new metabolites, metguignardic acid (4) and (-)-epi-guignardone I (14), were identified along the known dioxolanones guignardic acid (1), ethyl guignardate (3), guignardianones A (5), C (2), D (7), and E (6), phenguignardic acid methyl ester (8), the meroterpenes guignardone A (9) and B (10), guignarenone B (11) and C (12), (-)-guignardone I (13), and phyllomeroterpenoid B (15). Among these compounds, 1 and 4 were effective antifeedants against S. littoralis and M. persicae, while 2 was only active on the aphid M. persicae. The nematicidal compounds were 4, 7, and 8. This is the first report on the insect antifeedant or nematicidal effects of these dioxolanone-type compounds. Since the insect antifeedant and nematicidal activity of the Phyllosticta sp. extract depend on the presence of dioxolanone components, future fermentation optimizations are needed to promote the biosynthesis of these compounds instead of meroterpenes.
Platinum/Platinum Sulfide on Sulfur-Doped Carbon Nanosheets with Multiple Interfaces toward High Hydrogen Evolution Activity
Authors: Zhang Mou , Su Mengfei , Zhang Chunyan , Gao Feng , Lu Qingyi
Published: September 2024

Platinum (Pt)-based materials are among the most competitive electrocatalysts for the hydrogen evolution reaction (HER) due to suitable hydrogen adsorption energy. Due to the rarity of Pt, it is desirable to develop cost-effective Pt-based electrocatalysts with low Pt loading. Herein, Pt/PtS electrocatalysts on S-doped carbon nanofilms (PPS/C) have been successfully fabricated through a precursor reduction route with a complex of Pt and 1-dodecanethiol (1-DDT) as the precursor. The PPS/C achieved at 400 °C (PPS/C-400) exhibits excellent HER performances with an ultralow overpotential of 41.3 mV, a low Tafel slope of 43.1 mV dec−1 at a current density of 10 mA cm−2, and a long-term stability of 10 h, superior to many recently reported Pt-based HER electrocatalysts. More importantly, PPS/C-400 shows a high mass-specific activity of 0.362 A mgPt−1 at 30 mV, which is 1.88 times of that of commercial 20% Pt/C (0.193 A mgPt−1). The introduction of sulfur leads to the formation of PtS, which not only reduces the content of Pt but also realizes the interface regulation of Pt/PtS, as well as the doping of carbon. Both regulations make the resulting catalyst have abundant active centers and rapid electron transfer/transport, which is conducive to balancing the adsorption and resolution of intermediate products, and finally achieving great mass-specific activity and stability. The research work may provide ideas for designing effective Pt-based multi-interface electrocatalysts.
Rapid Concentration of Ga-68 and Proof-of-Concept Microscale Labeling of [68Ga]Ga-PSMA-11 in a Droplet Reactor
Authors: Lu Yingqing , Chao Philip H. , Collins Jeffrey , van Dam R. Michael
Published: September 2024

The radiometal gallium-68 (Ga-68) has garnered significant interest due to its convenient production via compact and widely available generators and the high performance of 68Ga-labeled compounds for positron-emission tomography (PET) imaging for cancer diagnosis and management of patients undergoing targeted radionuclide therapy. Given the short half life of Ga-68 (68 min), microfluidic-based radiosynthesis is a promising avenue to establish very rapid, efficient, and routine radiolabeling with Ga-68; however, the typical elution volume of Ga-68 from a generator (4–10 mL) is incompatible with the microliter reaction volumes of microfluidic devices. To bridge this gap, we developed a microscale cartridge-based approach to concentrate Ga-68. By optimizing cartridge design, resin type, resin mass, and eluent composition, Ga-68 was reliably concentrated from ~6 mL to ~80 µL with high recovery efficiency (>97%, n = 14). Furthermore, this method is suitable for both single- and dual-generator setups. To demonstrate suitability of the concentrated radiometal for radiolabeling, we performed microdroplet synthesis of [68Ga]Ga-PSMA-11, achieving high radiochemical yield (83 ± 11%, n = 3), excellent radiochemical purity (>99%), and high apparent specific activity (255–320 MBq/μg). The entire process, including Ga-68 concentration, radiosynthesis, purification, and formulation, was completed in 12 min. Starting with activity of 0.81–0.84 GBq, 0.51–0.64 GBq of product was produced, sufficient for multiple patient doses. This work paves the way to clinical-scale production of other 68Ga-labeled compounds using droplet microreactor methods, or high-throughput labeling optimization or compound screening of 68Ga-labeled probes using droplet reaction arrays.
Immunomodulatory Effects of Green Tea Catechins and Their Ring Fission Metabolites in a Tumor Microenvironment Perspective
Authors: Andrade Emmanuele D. S. , Santos Ronimara A. , Guillermo Landi V. C. , Miyoshi Noriyuki , Ferraz da Costa Danielly C.
Published: September 2024

Green tea is the second most consumed beverage following water, and the health benefits provided by its consumption have been well established from research in recent decades. The main bioactive compounds found in all Camellia sinensis-based teas are catechins, which have been reported to have antioxidant, anticancer, anti-inflammatory, and immunomodulatory properties. Although most of the health benefits are well established, studies show that the intact catechins as found in tea are poorly absorbed in the digestive tract. These compounds are degraded and undergo ring fission by the gut microbiota, increasing their absorption. In this review, we gather knowledge of the health benefits of green tea catechins and their metabolites, with a particular emphasis on the immunomodulatory effects in a cancer microenvironment scenario.
Functional Rehabilitation for Medial Gastrocnemius Silent Contractures to Prevent Foot and Ankle Disorders: A Review
Authors: Bernardes Rafael A. , Parola Vítor , Cruz Arménio , Correia Nuno , Neves Hugo
Published: September 2024

Medial gastrocnemius silent contractures (MGSCs) are prevalent, notably impacting functional status and increasing the risk of foot and ankle disorders, especially among aging populations. Although traditionally managed by podiatrists and physiotherapists, the role of rehabilitation nursing in addressing MGSCs is gaining recognition. This paper elucidates the contributions of rehabilitation nursing to the functional rehabilitation of MGSC patients and underscores its vital role within the multidisciplinary team. Initially, the paper defines the clinical and physiological characteristics of MGSCs and their implications in foot and ankle disorders. It then meticulously explores rehabilitation nursing interventions—including personalized stretching regimens, vibration therapy, balance exercises, and judicious footwear selection—emphasizing their efficacy in enhancing muscle flexibility, joint mobility, and postural stability. The emphasis is on patient-centered approaches and education to foster treatment adherence and positive rehabilitation outcomes. The significance of interdisciplinary collaboration is highlighted, focusing on how rehabilitation nursing optimizes patient care and mitigates complications. The paper advocates for recognizing and integrating rehabilitation nursing in managing MGSC-related disorders, emphasizing its importance in achieving successful functional outcomes.
Quantum Dot-Based Nanosensors for In Vitro Detection of Mycobacterium tuberculosis
Authors: Nikolaev Viktor V. , Lepekhina Tatiana B. , Alliluev Alexander S. , Bidram Elham , Sokolov Pavel M. , Nabiev Igor R. , Kistenev Yury V.
Published: September 2024

Despite the existing effective treatment methods, tuberculosis (TB) is the second most deadly infectious disease, its carriers in the latent and active phases accounting for more than 20% of the world population. An effective method for controlling TB and reducing TB mortality is regular population screening aimed at diagnosing the latent form of TB and taking preventive and curative measures. Numerous methods allow diagnosing TB by directly detecting Mycobacterium tuberculosis (M.tb) biomarkers, including M.tb DNA, proteins, and specific metabolites or antibodies produced by the host immune system in response to M.tb. PCR, ELISA, immunofluorescence and immunochemical analyses, flow cytometry, and other methods allow the detection of M.tb biomarkers or the host immune response to M.tb by recording the optical signal from fluorescent or colorimetric dyes that are components of the diagnostic systems. Current research in biosensors is aimed at increasing the sensitivity of detection, a promising approach being the use of fluorescent quantum dots as brighter and more photostable optical tags. Here, we review current methods for the detection of M.tb biomarkers using quantum dot-based nanosensors and summarize data on the M.tb biomarkers whose detection can be made considerably more sensitive by using these sensors.
Through the Intestines to the Head? That Is, How the Gastrointestinal Microbiota Affects the Behavior of Companion Animals
Authors: Gorzelanna Zofia , Miszczak Marta
Published: September 2024

Microbiome research has become significantly advanced in recent years, both in human and veterinary medicine. The development of DNA sequencing technology has enabled a deeper understanding of the diversity of microorganisms inhabiting animal bodies. It has given clinicians, scientists, and behaviorists a chance of better understanding the impact that a proper microbial population has on the individual, enabling a much more holistic view of the animals’ health and welfare. Current knowledge is increasingly based on analyses of the impact of microorganisms present in the gastrointestinal tract on the neurobiology and behavior of the host. This review presents research results, indicating potential implications for fields such as ethology and veterinary medicine. Understanding the role of the microbiome in shaping animal behavior could open up new therapeutic opportunities and lead to more sustainable animal husbandry practices.
Nd:YVO4 Random Laser with Preferential Emission at 1340 nm over 1064 nm
Authors: Dipold Jessica , Kassab Luciana R. P. , Wetter Niklaus U.
Published: September 2024

Neodymium-doped yttrium vanadate random lasers have presented exceptional efficiency and output power at the 1064 nm emission wavelength. However, emission at 1340 nm has not yet been observed for these random lasers, even though regular bulk lasers have presented many impressive properties in this infrared region. Here, we present a dual-emission Nd3+:YVO4 pellet random laser, which emits at both 1064 nm and 1340 nm using a 585 nm pump wavelength, showing a new property corresponding to a much lower laser threshold at 1340 nm than with 1064 nm.
Enhancing Tactile Internet Reliability: AI-Driven Resilience in NG-EPON Networks
Authors: Liem Andrew Tanny , Hwang I-Shyan , Kharga Razat , Teng Chin-Hung
Published: September 2024

To guarantee the reliability of Tactile Internet (TI) applications such as telesurgery, which demand extremely high reliability and are experiencing rapid expansion, we propose a novel smart resilience mechanism for Next-Generation Ethernet Passive Optical Networks (NG-EPONs). Our architecture integrates Artificial Intelligence (AI) and Software-Defined Networking (SDN)-Enabled Broadband Access (SEBA) platform to proactively enhance network reliability and performance. By harnessing the AI’s capabilities, our system automatically detects and localizes fiber faults, establishing backup communication links using Radio Frequency over Glass (RFoG) to prevent service disruptions. This empowers NG-EPONs to maintain uninterrupted, high-quality network service even in the face of unexpected failures, meeting the stringent Quality-of-Service (QoS) requirements of critical TI applications. Our AI model, rigorously validated through 5-fold cross-validation, boasts an average accuracy of 81.49%, with a precision of 84.33%, recall of 78.18%, and F1-score of 81.00%, demonstrating its robust performance in fault detection and prediction. The AI model triggers immediate corrective actions through the SDN controller. Simulation results confirm the efficacy of our proposed mechanism in terms of delay, system throughputs and packet drop rate, and bandwidth waste, ultimately ensuring the delivery of high-quality network services.
Design and Simulation of a High-Responsivity Dielectric Metasurface Si-Based InGaAs Photodetector
Authors: Dong Hengyang , Wu Yujie , Zheng Hongbo , Chen Pandi , Deng Wenhao , Ma Liuhong , Dong Xinyuan , Duan Zhiyong , Li Mengke
Published: September 2024

A Si-based photodetector is the core device of Si-based optical interconnection; its material and performance are the key factors restricting its development. This paper conducts theoretical research on the issues of lattice mismatch between heterogeneous materials and low device responsivity in Si-based InGaAs photodetectors for the 1550 nm optical communication band. The material mismatch issue is addressed through the use of the high-aspect ratio trapping (ART) epitaxial technique, enabling the realization of high-performance Si-based III-V materials. By introducing a dielectric metasurface into the top layer of the structure, the light absorption efficiency is enhanced, realizing broadband optical absorption enhancement for Si-based photodetectors. This paper mainly focuses on designing the optimal parameters of the dielectric metasurface structure based on the finite-difference time-domain (FDTD) Solutions to achieve the performance analysis of a high-responsivity 1550 nm Si-based InGaAs photodetector. The results show that the quantum efficiency of the dielectric metasurface structure is theoretically estimated to be 88.8% and the response rate is 1.11 A/W, which is 2%~16% higher than that of the unetched structure in the whole band. The research results of this paper will provide new ideas for the development of novel, high-performance, and miniaturized Si-based photodetectors and lay a theoretical foundation for Si-based optical interconnection.
Developmental Morphology, Physiology, and Molecular Basis of the Pentagram Fruit of Averrhoa carambola
Authors: Tuo Wanli , Wu Chunmei , Wang Xuexuan , Yang Zirui , Xu Lianhuan , Shen Siyuan , Zhai Junwen , Wu Shasha
Published: September 2024

Averrhoa carambola, a key tropical and subtropical economic tree in the Oxalidaceae family, is distinguished by its unique pentagram-shaped fruit. This study investigates the developmental processes shaping the polarity of A. carambola fruit and their underlying hormonal and genetic mechanisms. By analyzing the Y1, Y2, and Y3 developmental stages—defined by the fruit diameters of 3–4 mm, 4–6 mm, and 6–12 mm, respectively—we observed that both cell number and cell size contribute to fruit development. Our findings suggest that the characteristic pentagram shape is established before flowering and is maintained throughout development. A hormonal analysis revealed that indole-3-acetic acid (IAA) and abscisic acid (ABA) show differential distribution between the convex and concave regions of the fruit across the developmental stages, with IAA playing a crucial role in polar auxin transport and shaping fruit morphology. A transcriptomic analysis identified several key genes, including AcaGH3.8, AcaIAA20, AcaYAB2, AcaXTH6, AcaYAB3, and AcaEXP13, which potentially regulate fruit polarity and growth. This study advances our comprehension of the molecular mechanisms governing fruit shape, offering insights for improving fruit quality through targeted breeding strategies.
Establishment of a Sensitive and Reliable Droplet Digital PCR Assay for the Detection of Bursaphelenchus xylophilus
Authors: Su Yu , Zhu Xuedong , Jing Haozheng , Yu Haiying , Liu Huai
Published: September 2024

Pine wilt disease (PWD), which poses a significant risk to pine plantations across the globe, is caused by the pathogenic agent Bursaphelenchus xylophilus, also referred to as the pine wood nematode (PWN). A droplet digital PCR (ddPCR) assay was developed for the quick identification of the PWN in order to improve detection sensitivity. The research findings indicate that the ddPCR assay demonstrated significantly higher analysis sensitivity and detection sensitivity in comparison to traditional quantitative PCR (qPCR). However, it had a more limited dynamic range. High specificity was shown by both the ddPCR and qPCR techniques in the diagnosis of the PWN. Assessments of reproducibility revealed that ddPCR had lower coefficients of variation at every template concentration. Inhibition tests showed that ddPCR was less susceptible to inhibitors. There was a strong linear association between standard template measurements obtained using ddPCR and qPCR (Pearson correlation = 0.9317; p < 0.001). Likewise, there was strong agreement (Pearson correlation = 0.9348; p < 0.001) between ddPCR and qPCR measurements in the evaluation of pine wood samples. Additionally, wood samples from symptomatic (100% versus 86.67%) and asymptomatic (31.43% versus 2.9%) pine trees were diagnosed with greater detection rates using ddPCR. This study’s conclusions highlight the advantages of the ddPCR assay over qPCR for the quantitative detection of the PWN. This method has a lot of potential for ecological research on PWD and use in quarantines.
Challenges and Advances in Tertiary Waste Water Treatment for Municipal Treatment Plants
Authors: Solcova Olga , Dlaskova Martina , Kastanek Frantisek
Published: September 2024

Municipal waste water treatment plants have a fundamental task, which is to rid waste water of toxic and health-threatening organic and inorganic substances, including unwanted microorganisms and other pollutants, with the highest possible efficiency so that the discharged water does not contaminate the natural environment, which happens in the case of imperfect cleaning. Current WWTPs usually have a preliminary, primary, and secondary stage, and in very few cases even a tertiary stage, which no longer guarantees the sufficient removal of pollutants from waste water. This research presents the current situation in different parts of the world concerning the possibility of solving the current situation regarding the tertiary and quaternary stages of this process, especially in small and rural WWTPs serving up to approx. 10,000 equivalent inhabitants, which could ensure the removal of so-called emerging pollutants, including microplastics, and would stop WWTPs being point sources of environmental contamination.
Innovations in Food Packaging: From Bio-Based Materials to Smart Packaging Systems
Authors: D’Almeida Alan Portal , de Albuquerque Tiago Lima
Published: September 2024

This review highlights recent innovations in food packaging, emphasizing the shift from conventional petroleum-based materials to bio-based alternatives and smart packaging systems. Bio-based materials, such as starch, cellulose, and polyhydroxyalkanoates (PHA), offer sustainable solutions due to their biodegradability and reduced environmental impact. These materials are positioned as eco-friendly alternatives to traditional plastics but face challenges related to production costs and scalability. Additionally, advancements in smart packaging technologies, including sensor and indicator systems, provide real-time food quality monitoring, enhancing food safety and reducing waste. Active packaging technologies, incorporating natural antioxidants and moisture control, extend product shelf life and improve food preservation. Furthermore, these biopolymers typically present a lower CO2 footprint, energy costs, and water consumption during production, compared to traditionally used synthetic plastics. The review identifies challenges, such as regulatory barriers and technological limitations, but also outlines significant opportunities for future research and innovation in the food packaging sector, aiming for more efficient, safer, and environmentally sustainable packaging solutions.
Esterification Process in Supercritical Carbon Dioxide Catalyzed by Geotrichum candidum Lipase Produced with Mozzarella Cheese Whey and Corn Steep Liquor
Authors: Ramos Paulo Rodolfo , de Oliveira Alessandra Lopes , Ramos Giselle Vallim Correa , Kamimura Eliana Setsuko
Published: September 2024

The valorization of industrial waste in the production of new products is a growing trend, with food waste showing significant promise as a raw material for various industries. Thus, this research aimed to investigate the production of Geotrichum candidum lipase using industrial waste, such as mozzarella cheese whey (MCW) and corn steep liquor (CSL), and to analyze how effectively it catalyzes the esterification of oleic acid with methanol. Lipase production was carried out in medium containing MCW and CSL, with fermentation conditions optimized using a fractional factorial experimental design and central composite experimental design. The highest activities (16.71 U/mL in 24 h and 17.80 U/mL in 48 h) were found in conditions of 13.6% (%w/v) CSL and 37.1% (%w/v) MCW, with corn oil fixed at 1% (%w/v) and pH fixed at 6,00. Esterification conditions were evaluated at atmospheric pressure, both in a solvent-free environment and using hexane, as well as under high pressures with supercritical carbon dioxide (SC-CO2). The produced lipase demonstrated high catalytic activity for the esterification reaction of oleic acid with methanol under SC-CO2 with an increase of 39.41% in the yield. The yields found confirm the feasibility of producing lipase from industrial waste, thus presenting it as a sustainable and efficient alternative for industrial processes, and show that there is no need to use toxic organic solvents in esterification reactions.
Study on the Fracture Evolution Characteristics of Overlying Strata in a Fully Mechanized Mining Face with a Large Mining Height Based on a Three-Dimensional Large-Scale Physical Simulation Experimental System
Authors: Wei Zongyong , Yin Yucai , Li Botao , Li Shugang , Lin Haifei , Xiao Peng , Ding Yang
Published: September 2024

To investigate the evolution characteristics of overlying rock fractures, based on a geological prototype of a large-height comprehensive mining face in Shanxi, a three-dimensional large-scale physical similarity model was established. The experiments were carried out using microseismic monitoring and physical model cutting methods to study the activity and fissure evolution of the overburden rock. Model cutting revealed that, approximately 65 m from the bottom of the coal seam, delamination occurred, marking the top of the overburden rock fissure zone and the bottom of the bending and sinking zone. At 25 m from the coal seam bottom, the rock layer was highly fragmented, forming the collapse zone, which was 4.8 times the mining height. Between 25 and 65 m from the bottom, a fissure zone existed, which was 12.5 times the mining height, with abundant delamination fissures at the top of the fissure zone. Significant microseismic events were observed as the coal face advanced to 45 m, with notable increases in the concentrations and distribution ranges of these events in both the strike and height directions of the coal seam. The subsidence range of the overlying rock layer expanded from the top to the bottom, with the subsidence slope area extending gradually and the central compaction area remaining relatively flat. The overall shape presented an irregular ellipse, with peripheral uplift phenomena observed in the subsidence area. At 39 m from the coal seam bottom, the maximum subsidence of the rock stratum was 4.0 m, with subsidence amounts decreasing with increasing stratum height. Fissure density along the coal seam inclination and direction exhibited a double hump pattern, with fissure areas on both sides showing high densities and the central compaction areas having low densities. Coal seam mining caused stress redistribution in the surrounding rock layer, and the stress in front of the work was divided into the stress reduction zone, dynamic influence zone, mining influence zone, and unaffected zone. Coal rock porosity under high stress was less sensitive to stress changes, resulting in smaller changes in fissure permeability and fissures remaining mostly closed.