bluuebunny/crossref_metadata_2025 huggingface dataset:
Construction of HER2-Specific HIV-1-Based VLPs
Authors: Martins Sofia A. , Santos Joana , Cabo Verde Sandra , Correia João D. G. , Melo Rita
Published: November 2022

Virus-like particles (VLPs) are nanoplatforms comprised of one or more viral proteins with the capacity to self-assemble without viral genetic material. VLPs arise as promising nanoparticles (NPs) that can be exploited as vaccines, as drug delivery vehicles or as carriers of imaging agents. Engineered antibody constructs, namely single-chain variable fragments (scFv), have been explored as relevant molecules to direct NPs to their target. A vector containing the scFv of an antibody, aimed at the human epidermal growth factor receptor 2 (HER2) and fused to the human immunodeficiency virus (HIV) protein gp41, was previously constructed. The work herein describes the early results concerning the production and the characterization of HIV-1-based VLPs expressing this protein, which could function as potential non-toxic tools for transporting drugs and/or imaging agents.
Estuarine Aquacultures at the Crossroads of Animal Production and Antibacterial Resistance: A Metagenomic Approach to the Resistome
Authors: Silva Daniel G. , Domingues Célia P. F. , Figueiredo João F. , Dionisio Francisco , Botelho Ana , Nogueira Teresa
Published: November 2022

It is recognized that the spread of antibiotic resistance (AR) genes among aquatic environments, including aquaculture and the human environment, can have detrimental effects on human and animal health and the ecosystem. Thus, when transmitted to the human microbiome or pathogens, resistance genes risk human health by compromising the eventual treatment of infections with antibiotic therapy. This study aimed to define the resistance profile of aquaculture farms and their potential risk for spreading. Twenty-four sediments from oyster and gilthead sea bream aquaculture farms located in three Portuguese river estuaries (17 sediments from Sado, 4 from Aveiro, and 3 from Lima) were studied by comparative metagenomic analysis. The computation of the diversity of genes conferring resistance per antibiotic class revealed a significant increase in aminoglycosides, beta-lactams, disinfectants, quinolones, and tetracyclines counts. In all geographic locations under study, the most diverse AR genes confer resistance to the macrolides, tetracyclines and oxazolidinones classes, all of which are medically important for human and animal therapies, as well as resistance to disinfectants. The diversity of mobile genetic elements correlated with the number of AR genes such as tetracyclines, suggesting that AR could be easily mobilized among bacterial genomes and microbiomes.
Hybrid-Enhanced Siamese Similarity Models in Ligand-Based Virtual Screen
Authors: Altalib Mohammed Khaldoon , Salim Naomie
Published: November 2022

Information technology has become an integral aspect of the drug development process. The virtual screening process (VS) is a computational technique for screening chemical compounds in a reasonable amount of time and cost. The similarity search is one of the primary tasks in VS that estimates a molecule’s similarity. It is predicated on the idea that molecules with similar structures may also have similar activities. Many techniques for comparing the biological similarity between a target compound and each compound in the database have been established. Although the approaches have a strong performance, particularly when dealing with molecules with homogenous active structural, they are not enough good when dealing with structurally heterogeneous compounds. The previous works examined many deep learning methods in the enhanced Siamese similarity model and demonstrated that the Enhanced Siamese Multi-Layer Perceptron similarity model (SMLP) and the Siamese Convolutional Neural Network-one dimension similarity model (SCNN1D) have good outcomes when dealing with structurally heterogeneous molecules. To further improve the retrieval effectiveness of the similarity model, we incorporate the best two models in one hybrid model. The reason is that each method gives good results in some classes, so combining them in one hybrid model may improve the retrieval recall. Many designs of the hybrid models will be tested in this study. Several experiments on real-world data sets were conducted, and the findings demonstrated that the new approaches outperformed the previous method.
Itraconazole and Difluorinated-Curcumin Containing Chitosan Nanoparticle Loaded Hydrogel for Amelioration of Onychomycosis
Authors: Kesharwani Prashant , Fatima Mahak , Singh Vanshikha , Sheikh Afsana , Almalki Waleed H. , Gajbhiye Virendra , Sahebkar Amirhossein
Published: November 2022

Onychomycosis is a nail infection caused by a fungus, Trichophyton mentagrophytes, that is responsible for major nail infections. The best method suited for treating such infections generally includes a topical remedy. However, conventional oral or topical formulations are associated with various limitations. Therefore, a more efficient and compatible formulation is developed in this study. The primary objective of the current study is to formulate and evaluate chitosan nanoparticle-based hydrogel for ameliorating onychomycosis. The sole purpose of this research was to increase the permeation of the lipophilic drug itraconazole and difluorinated curcumin, and its synergistic antifungal activity was also evaluated for the first time. Both in vitro and ex vivo drug release evaluations confirmed the sustained release of both drugs from the hydrogel, which is a prerequisite for treating onychomycosis. The results overall highlighted the promising activity of a synergistic approach that could be implemented for the treatment of onychomycosis. The hydrogel-based formulation serves as an effective method of delivery of drugs across the layers of the skin, resulting from its hydrating characteristics.
Label-Free Sensing of Biomolecular Adsorption and Desorption Dynamics by Interfacial Second Harmonic Generation
Authors: Xia Chuansheng , Sun Jianli , Wang Qiong , Chen Jinping , Wang Tianjie , Xu Wenxiong , Zhang He , Li Yuanyuan , Chang Jianhua , Shi Zengliang , Xu Chunxiang , Cui Qiannan
Published: November 2022

Observing interfacial molecular adsorption and desorption dynamics in a label-free manner is fundamentally important for understanding spatiotemporal transports of matter and energy across interfaces. Here, we report a label-free real-time sensing technique utilizing strong optical second harmonic generation of monolayer 2D semiconductors. BSA molecule adsorption and desorption dynamics on the surface of monolayer MoS2 in liquid environments have been all-optically observed through time-resolved second harmonic generation (SHG) measurements. The proposed SHG detection scheme is not only interface specific but also expected to be widely applicable, which, in principle, undertakes a nanometer-scale spatial resolution across interfaces.
EEG Network Analysis in Epilepsy with Generalized Tonic–Clonic Seizures Alone
Authors: Pitetzis Dimitrios , Frantzidis Christos , Psoma Elizabeth , Deretzi Georgia , Kalogera-Fountzila Anna , Bamidis Panagiotis D. , Spilioti Martha
Published: November 2022

Many contradictory theories regarding epileptogenesis in idiopathic generalized epilepsy have been proposed. This study aims to define the network that takes part in the formation of the spike-wave discharges in patients with generalized tonic–clonic seizures alone (GTCSa) and elucidate the network characteristics. Furthermore, we intend to define the most influential brain areas and clarify the connectivity pattern among them. The data were collected from 23 patients with GTCSa utilizing low-density electroencephalogram (EEG). The source localization of generalized spike-wave discharges (GSWDs) was conducted using the Standardized low-resolution brain electromagnetic tomography (sLORETA) methodology. Cortical connectivity was calculated utilizing the imaginary part of coherence. The network characteristics were investigated through small-world propensity and the integrated value of influence (IVI). Source localization analysis estimated that most sources of GSWDs were in the superior frontal gyrus and anterior cingulate. Graph theory analysis revealed that epileptic sources created a network that tended to be regularized during generalized spike-wave activity. The IVI analysis concluded that the most influential nodes were the left insular gyrus and the left inferior parietal gyrus at 3 and 4 Hz, respectively. In conclusion, some nodes acted mainly as generators of GSWDs and others as influential ones across the whole network.
Neurosurgical Treatment of Pain
Authors: Sola Rafael G. , Pulido Paloma
Published: November 2022

The aim of this review is to draw attention to neurosurgical approaches for treating chronic and opioid-resistant pain. In a first chapter, an up-to-date overview of the main pathophysiological mechanisms of pain has been carried out, with special emphasis on the details in which the surgical treatment is based. In a second part, the principal indications and results of different surgical approaches are reviewed. Cordotomy, Myelotomy, DREZ lesions, Trigeminal Nucleotomy, Mesencephalotomy, and Cingulotomy are revisited. Ablative procedures have a limited role in the management of chronic non-cancer pain, but they continues to help patients with refractory cancer-related pain. Another ablation lesion has been named and excluded, due to lack of current relevance. Peripheral Nerve, Spine Cord, and the principal possibilities of Deep Brain and Motor Cortex Stimulation are also revisited. Regarding electrical neuromodulation, patient selection remains a challenge.
Consumer Response to Food Corporate Social Irresponsibility: Food Performance and Company Ethics Irresponsibility
Authors: Yu Weiping , Si Dongyang , Zhou Jun
Published: November 2022

Corporate social irresponsibility (CSI) seriously damages the rights and interests of stakeholders, particularly consumers. This study analyzes the consumer response to food performance irresponsibility and food corporate ethics irresponsibility by moral emotions. A situational simulation experiment was conducted with the following results: (1) Food performance irresponsibility has the greatest impact on consumer boycotts, while corporate ethics irresponsibility more often leads to consumers’ negative word of mouth (NWOM). (2) Moral emotions play a strong mediating role between CSI and consumers’ NWOM and boycott behavior. (3) Gender significantly moderates the propagation path from moral emotions to NWOM, and female consumers react more strongly to food performance irresponsibility. In conclusion, the paper offers empirical evidence of the effect food corporate social irresponsibility has on consumers’ different responses. Furthermore, it can help food enterprises to identify different CSI types and develop corresponding governance strategies.
Direct and Indirect Effects of Child Maltreatment on Suicidal Ideation among Chinese Left-Behind Children: Does Gender Make a Difference?
Authors: Fan Xiaoyan , Li Xiangping
Published: November 2022

Previous studies indicate that maltreatment is related to children’s suicidal ideation, but the indirect mechanisms of left-behind children have been rarely investigated in the Chinese context. On the basis of a left-behind children sample (N = 1355; 57.1% females), this study aims to investigate the direct and indirect effects of maltreatment on suicidal ideation among Chinese left-behind children. The results of structural equation modeling (SEM) demonstrate that child maltreatment not only directly affects the suicidal ideation of left-behind children, but can also indirectly affect their suicidal ideation through the partially mediating role of self-efficacy. Moreover, a significant gender difference was found in the direct effect of maltreatment on suicidal ideation, with females experiencing stronger influence than males. Findings suggest that the effect of maltreatment on the suicidal ideation of left-behind children is mediated by self-efficacy and moderated by gender. Findings also highlight intervention directions for risk behaviors among left-behind children.
Development of a Solar Tracking-Based Movable Louver System to Save Lighting Energy and Create a Comfortable Light Environment
Authors: Jung Su-yeon , Han Sowon , Lee Min-Goo , Lee Heangwoo
Published: November 2022

Louvers are among the technical components considered for improving energy performance in buildings, and there has been increased interest in adapting the louver system. However, most previous studies have focused on their performance evaluation based on the width and angle of the slats, which allow for limited improvement in their efficiency. This study suggests a solar tracking-based movable louver (STML) system and examines the efficacy using a full-scale test bed. To do so, we developed a full-scale test bed and estimated the energy reduction and improvement of indoor uniformity of different types of STML systems, including vertical, horizontal, eggcrate, and hybrid. The main findings are as follows: (1) The proposed STML is a hybrid louver with four movable shafts due to its structural characteristics. The shading area is increased sequentially by controlling the length of the movable shaft adjacent to the sun through solar tracking. (2) Compared with conventional vertical and horizontal louvers, the STML can improve indoor uniformity by 5.0% and 13.9%, respectively. Unlike conventional louvers, the STML awnings are installed at the end of the daylighting window, reducing window view obstruction and creating a more pleasant indoor visual environment. (3) Compared with conventional louvers, the STML can reduce lighting and heating/cooling energy by 35.7–49.7%. These findings prove the effectiveness of the proposed system.
An Integrated Approach of Simulation and Regression Analysis for Assessing Productivity in Modular Integrated Construction Projects
Authors: Taiwo Ridwan , Hussein Mohamed , Zayed Tarek
Published: November 2022

Many nations across the globe face the challenge of housing deficit. Modular integrated construction (MiC), which has the highest level of prefabrication among off-site construction manufacturing (OSM), has been adopted as a fast and reliable construction method to address the housing deficit. Previous studies have assessed the productivity of the prefabrication stage of MiC, while investigations into the productivity of the MiC installation process with the consideration of pragmatic factors, especially for high-rise buildings, are lacking in the literature. Therefore, this study contributes by (1) developing a discrete-event simulation (DES) model to assess the productivity of MiC installation while considering pragmatic factors (e.g., weather conditions, topography, work dimension, etc.) and management conditions (e.g., workers’ motivation, training, equipment maintenance, etc.); (2) developing a mathematical model to understand the relationship between productivity and various resources utilized in MiC installation. After verifying and validating the DES model, it was applied to a case study in Hong Kong. A sensitivity analysis using a full factorial experiment design was conducted to identify the parameters (e.g., number of trucks, tower cranes, different crews) that significantly affect a number of performance measures, such as the project duration, productivity, and total costs. Furthermore, the mathematical model shows high prediction accuracy, as the mean absolute percentage error is 8.93%. This study would help construction practitioners in their decision-making process, while planning a project by providing them with a model that can predict the productivity of the MiC installation process before and during the project implementation.
Effect of the Openings on the Seismic Response of an Infilled Reinforced Concrete Structure
Authors: Furtado André , Rodrigues Hugo , Arêde António
Published: November 2022

The seismic behavior of the infill masonry infill walls has a significant impact on the global response of reinforced concrete frame structures. One factor influencing its behavior is the existence of openings in the walls, such as doors and windows, which are crucial for the infill seismic performance. Although the numerical simulation of the seismic behavior of RC buildings with infill walls has evolved significantly in recent years in terms of micro- and macro-modelling, most of the existing studies are only related to infill walls without openings. Based on this motivation, four main objectives were defined for this research work: (i) present a simplified modeling approach and its calibration to simulate the seismic behavior of infill walls with central openings such as windows; (ii) evaluate the impact of the openings on the global seismic response of an RC building; (iii) study the impact of the irregular distribution of the infill walls (vertical and in-plane) on the global seismic response of an RC building; and (iv) study the impact of the central openings ratio (i.e., relative percentage between opening and infill wall area) on the global seismic response of an RC building structure. A four-story infilled RC building was used as a case study to perform parametric analyses investigating the impact of the masonry infill walls’ irregular distribution (vertical and in-plan) and their openings ratio. The results are discussed in terms of natural frequencies and vibration modes, initial lateral stiffness, and maximum lateral resistance. This study found that the openings caused a reduction in the natural frequencies of about 20% compared with the full infill (without openings). The openings did not modify the vibration modes. In addition, the openings reduced the initial stiffness by about 20% compared with the model without openings. The maximum strength increased about 50% with the infill walls, but this was reduced by the openings by 20%.
Dynamic Characteristic Monitoring of Wind Turbine Structure Using Smartphone and Optical Flow Method
Authors: Zhao Wenhai , Li Wanrun , Fan Boyuan , Du Yongfeng
Published: November 2022

The dynamic characteristics of existing wind turbine structures are usually monitored using contact sensors, which is not only expensive but also time-consuming and laborious to install. Recently, computer vision technology has developed rapidly, and monitoring methods based on cameras and UAVs (unmanned aerial vehicles) have been widely used. However, the high cost of UAVs and cameras make it difficult to widely use them. To address this problem, a target-free dynamic characteristic monitoring method for wind turbine structures using portable smartphone and optical flow method is proposed by combining optical flow method with robust corner feature extraction in ROI (region of interest). Firstly, the ROI region clipping technology is introduced after the structural vibration video shooting, and the threshold value is set in the ROI to obtain robust corner features. The sub-pixel displacement monitoring is realized by combining the optical flow method. Secondly, through three common smartphone shooting state to monitor the structural displacement, the method of high pass filtering combined with adaptive scaling factor is used to effectively eliminate the displacement drift caused by the two shooting states of standing and slightly walking, which can meet the requirements of structural dynamic characteristics monitoring. After that, the structural displacement is monitored by assembling the telephoto lens on the smartphone. The accuracy of displacement monitored by assembling the telephoto lens on the smartphone is investigated. Finally, the proposed monitoring method is verified by the shaking table test of the wind turbine structure. The results show that the optical flow method, combined with smartphones, can accurately identify the dynamic characteristics of the wind turbine structure, and the smartphone equipped with a telephoto lens is more conducive to achieving low-cost wind turbine structure dynamic characteristics monitoring. This research can provide a reference for evaluating the condition of wind turbine structures.
Research on the Soil-Plugging Effect on Small-Diameter Jacked Piles through In Situ Testing and DEM Simulation
Authors: Wang Xueyan , Mei Yuan , Yuan Yili , Wang Rong , Zhou Dongbo
Published: November 2022

Small-diameter jacked piles are widely used in civil engineering. The formation and development of the soil-plugging effect and surface frictional behavior of jacked piles have a high impact on the construction process and pile quality. Clarifying the developmental pattern of the soil-plugging effect and the change law of frictional force forms the premise of scientific construction and construction quality. Firstly, we carried out two groups of in situ tests on the small-diameter jacked piles, recording the relationship between penetration depth and resistance force. Then, the discrete element method (DEM) was used to analyze the mechanical behavior of the small-diameter jacked piles during the construction process. The particle flow code (PFC) 2D was used to carry out the DEM simulation. The research results show that pile resistance exhibited an irregular development trend as the construction process proceeded. There is a sudden change in pile resistance when the pile tip reaches the interface of certain soil layers. Both tests revealed the same phenomenon, yet both occurred at different depths. The DEM analysis showed that plug sliding was the main reason for the above phenomenon. The difference in strength and stiffness of adjacent soil layers causes the soil plug to slide, leading to a sudden change in pile resistance. When the upper layer is soft and the layer below is hard, this phenomenon is especially obvious. This also leads to a difference in the location of the sudden change in pile resistance between the two groups of tests. The research results of this paper can be helpful for revealing the relationship between the soil-plugging effect of small-diameter jacked piles and the development of pile resistance and also provides a reference for relevant engineering construction and design.
Post-Earthquake Assessment and Strengthening of a Cultural-Heritage Residential Masonry Building after the 2020 Zagreb Earthquake
Authors: Ademović Naida , Toholj Mirko , Radonić Dalibor , Casarin Filippo , Komesar Sanda , Ugarković Karlo
Published: November 2022

After a long period of no excessive ground shaking in Croatia and the region of ex-Yugoslavia, an earthquake that woke up the entire region was the one that shook Croatia on 22 March 2020. More than 25,000 buildings were severely damaged. A process of reconstruction and strengthening of existing damaged buildings is underway. This paper presents proposed strengthening measures to be conducted on a cultural-historical building located in the city of Zagreb, which is under protection and located in zone A. After a detailed visual inspection and on-site experimental investigations, modeling of the existing and strengthened structure was performed in 3Muri. It is an old unreinforced masonry building typical not only for this region but for relevant parts of Europe (north, central, and east). The aim was to strengthen the building to Level 3 while respecting the ICOMOS recommendations and Venice Charter. Some non-completely conservative concessions had to be made, to fully retrofit the building as requested. The structural strengthening consisted of a series of organic interventions relying on—in the weakest direction—a new steel frame, new steel-ring frames, and FRCM materials, besides fillings the cracks. Such intervention resulted in increasing the ultimate load in the X and Y directions, respectively, more than 650 and 175% with reference to the unstrengthened structure. Good consistency was obtained between the numerical modeling, visual inspection, and on-site testing.
The Seismic Performance and Global Collapse Resistance Capacity of Infilled Reinforced Concrete Frames Considering the Axial–Shear–Bending Interaction of Columns
Authors: Huang Linjie , Han Jianping , Wen Hongwei , Li Chunyu , He Haocheng , Luo Yuxin , Qian Zhendong
Published: November 2022

This paper presents a mechanism and method for simulating the axial–shear–bending interaction of a reinforced concrete (RC) column. The three-dimensional model of a multi-story infilled RC frame was modeled using the OpenSees software. Static pushover and nonlinear dynamic analyses under fortification and rare earthquakes were conducted using the model. Finally, based on the incremental dynamic analyses of 22 suites of ground-motion records, the global collapse resistance capacity of the infilled RC frame was evaluated using the evaluation method of a normal distribution. The analytical results show that the axial–shear–bending interaction is a key factor that affects the seismic response of infilled RC frames. Under the fortification earthquake condition, no obvious damage to physical structures was evident; the influence was relatively minor. However, under the condition of a rare earthquake, severe damage to physical structures was evident, resulting in the underestimation of the lateral inter-story drift ratio, while the degradation rates of the load capacity and global collapse resistance capacities for the infilled concrete frames were highly overestimated when the axial–shear–bending interaction was not considered.
Effects of Openings and Axial Load Ratio on the Lateral Capacity of Steel-Fiber-Reinforced Concrete Shear Walls
Authors: Lin Zhou , Zhang Hongmei , Monti Giorgio , Castoro Chiara
Published: November 2022

Shear walls are commonly adopted as main structural members to resist vertical and lateral forces, thanks to their high load capacity and high lateral stiffness. However, their lateral capacity can be impaired in the presence of openings, which can reduce their lateral load capacity and stiffness. A possible solution is to cast shear walls using steel-fiber-reinforced concrete (SFRC), which effectively improves the deformation capacity of shear walls. However, few studies deal with the performance of such SFRC shear walls in the presence of openings. Moreover, the effect of different axial load ratios (ALR) is still not fully known. To study these essential parameters, a detailed Finite Element model has been implemented in ABAQUS. Having validated its accuracy against experimental tests on four SFRC shear walls, with and without openings, it has been subsequently used in a parametric study to analyze the effects of different ALRs, of different opening configurations, and of different reinforcement ratios. It is shown that door openings have a more detrimental effect on the lateral load capacity than window openings and that higher ALR values switch the prevailing failure mechanism from flexural to shear, thus reducing both ductility and deformation capacity.
From Maslow to Architectural Spaces: The Assessment of Reusing Old Industrial Buildings
Authors: Zheng Xuesen , Heath Timothy , Guo Sifan
Published: November 2022

In many cases, the purpose of reusing old industrial buildings is to serve the public. Converting a building that had a particular function and is unfamiliar to the public to a civic building is a great challenge. Significantly, the public’s curiosity towards a special-purpose industrial building alone is not enough to give the building a long life following its conversion and regeneration. To be sustainable in public life, the design of reused old industrial buildings should also meet the needs of the public. It is important to realise, however, that everyone’s needs are different. This paper will therefore analyse whether Abraham Maslow’s (1943) famous psychological theory, the Hierarchy of Needs, which not only summarized the content of human needs, but also divided them into levels, can be applied to the reuse of former industrial buildings. The paper translates this into the field of architecture to develop an assessment framework for the reuse of old industrial buildings. This assessment framework is able to combine mathematical models for quantitative assessment of future projects, and uses Shanghai 1933 Old Millfun, China as a case study for its demonstration. This provides an evaluation of a completed project and identifies challenges for further development, as well as providing guidance for future adaptive reuse projects.
Obesity-Associated ECM Remodeling in Cancer Progression
Authors: Li Junyan , Xu Ren
Published: November 2022

Adipose tissue, an energy storage and endocrine organ, is emerging as an essential player for ECM remodeling. Fibrosis is one of the hallmarks of obese adipose tissue, featuring excessive ECM deposition and enhanced collagen alignment. A variety of ECM components and ECM-related enzymes are produced by adipocytes and myofibroblasts in obese adipose tissue. Data from lineage-tracing models and a single-cell analysis indicate that adipocytes can transform or de-differentiate into myofibroblast/fibroblast-like cells. This de-differentiation process has been observed under normal tissue development and pathological conditions such as cutaneous fibrosis, wound healing, and cancer development. Accumulated evidence has demonstrated that adipocyte de-differentiation and myofibroblasts/fibroblasts play crucial roles in obesity-associated ECM remodeling and cancer progression. In this review, we summarize the recent progress in obesity-related ECM remodeling, the mechanism underlying adipocyte de-differentiation, and the function of obesity-associated ECM remodeling in cancer progression.
The Impact of Clonal Hierarchy and Heterogeneity on Phenotypic Manifestations of Myelodysplastic Neoplasms
Authors: El Hussein Siba , Loghavi Sanam
Published: November 2022

Until recently, conventional prognostication of myelodysplastic neoplasms (MDS) was performed using the revised International Prognostic Scoring System (IPSS-R), with additional adverse prognoses conferred by select mutations. Nonetheless, the clonal diversity and dynamics of coexisting mutations have been shown to alter the prognosis and treatment response in patients with MDS. Often in the process of clonal evolution, various initial hits are preferentially followed by a specific spectrum of secondary alterations, shaping the phenotypic and biologic features of MDS. Our ability to recapitulate the clonal ontology of MDS is a necessary step toward personalized therapy and the conceptualization of a better classification system, which ideally would take into consideration all genomic aberrations and their inferred clonal architecture in individual cases. In this review, we summarize our current understanding of the molecular landscape of MDS and the role of mutational combinations, clonal burden, and clonal hierarchy in defining the clinical fate of the disease.
Magnetron Sputtered Al Co-Doped with Zr-Fe2O3 Photoanode with Fortuitous Al2O3 Passivation Layer to Lower the Onset Potential for Photoelectrochemical Solar Water Splitting
Authors: Koh Tae Sik , Anushkkaran Periyasamy , Hwang Jun Beom , Choi Sun Hee , Chae Weon-Sik , Lee Hyun Hwi , Jang Jum Suk
Published: November 2022

In this paper, we investigate the magnetron sputtering deposition of an Al-layer on Zr-doped FeOOH (Zr-FeOOH) samples to fabricate a Zr/Al co-doped Fe2O3 (Al-Zr/HT) photoanode. An Al-layer is deposited onto Zr-FeOOH through magnetron sputtering and the thickness of the Al deposition is regulated by differing the sputtering time. Electrochemical impedance spectroscopy, intensity-modulated photocurrent spectroscopy, Mott-Schottky and time-resolved photoluminescence spectra analyses were used to study, in depth, the correlations between sputtered Al-layer thicknesses and PEC characteristics. High-temperature quenching (800 °C) assists in diffusing the Al3+ in the bulk of the Zr-doped Fe2O3 photoanode, whilst an unintended Al2O3 passivation layer forms on the surface. The optimized Al-Zr/HT photoelectrode achieved 0.945 mA/cm2 at 1.0 VRHE, which is 3-fold higher than that of the bare Zr/HT photoanode. The Al2O3 passivation layer causes a 100 mV cathodic shift in the onset potential. Al co-doping improved the donor density, thus reducing the electron transit time. In addition, the passivation effect of the Al2O3 layer ameliorated the surface charge transfer kinetics. The Al2O3 passivation layer suppressed the surface charge transfer resistance, consequently expediting the hole migration from photoanode to electrolyte. We believe that the thickness-controlled Al-layer sputtering approach could be applicable for various metal oxide photoanodes to lower the onset potential.
Kinetic Modelling of Esterification and Transesterification Processes for Biodiesel Production Utilising Waste-Based Resource
Authors: Hazrat M. A. , Rasul Mohammad G. , Khan Mohammad M. K. , Ashwath Nanjappa , Silitonga Arridina S. , Fattah I. M. R. , Mahlia T. M. Indra
Published: November 2022

Process optimisation and reaction kinetic model development were carried out for two-stage esterification-transesterification reactions of waste cooking oil (WCO) biodiesel. This study focused on these traditional processes due to their techno-economic feasibility, which is an important factor before deciding on a type of feedstock for industrialisation. Four-factor and two-level face-centred central composite design (CCD) models were used to optimise the process. The kinetic parameters for the esterification and transesterification processes were determined by considering both pseudo-homogeneous irreversible and pseudo-homogeneous first-order irreversible processes. For the esterification process, the optimal conditions were found to be an 8.12:1 methanol to oil molar ratio, 1.9 wt.% of WCO for H2SO4, and 60 °C reaction temperature for a period of 90 min. The optimal process conditions for the transesterification process were a 6.1:1 methanol to esterified oil molar ratio, 1.2 wt.% of esterified oil of KOH, reaction temperature of 60 °C, and a reaction time of 110 min in a batch reactor system; the optimal yield was 99.77%. The overall process conversion efficiency was found to be 97.44%. Further research into reaction kinetics will aid in determining the precise reaction process kinetic analysis in future.
Photocatalytic Degradation of Methyl Orange Dyes Using Green Synthesized MoS2/Co3O4 Nanohybrids
Authors: Tien Tsung-Mo , Chen Chao-Hsiang , Huang Chen-Tang , Chen Edward L.
Published: November 2022

In this work, a new binary MoS2/Co3O4 nanohybrids was successfully fabricated and the chemical structures, morphologies, electrochemical and optical characterizations were carried out. In addition, heterojunction nanoparticles present in S-scheme structures act as electron traps and promote light absorption capacity for the degradation of Methyl orange (MO) with visible-light activity. MoS2/Co3O4 nanohybrids suggested excellent photocatalytic performance compared to bare MoS2 and Co3O4, where 95.6% of MO was degraded within 170 min, respectively. The results also showed excellent stability and recyclability over five consecutive cycles, without noticeable changes in the nanocomposite structure. The boosted photocatalytic degradation and redox activities of MoS2/Co3O4 can be attributed to the created S-scheme heterostructure to facilitate the separation of and to delay recombination of photoinduced charge carriers. We believe that this strategy of exploiting nanohybrid photocatalysts has great potential in the field of environmental catalysis and diverse applications.
CdS Nanoparticles Decorated 1D CeO2 Nanorods for Enhanced Photocatalytic Desulfurization Performance
Authors: Lu Xiaowang , Liu Zhengwei , Zhao Xiangping , Xu Weiye , Hou Haijun , Qian Junchao
Published: November 2022

CdS nanoparticles were constructed onto one-dimensional (1D) CeO2 nanorods by a two-step hydrothermal method. The X-ray diffraction (XRD), transmission election microscopy (TEM), Raman spectra, X-ray photoelectron spectra (XPS) and UV-Vis diffuse reflection spectroscopy (DRS) techniques were used to characterize these CdS/CeO2 nanocomposites. It was concluded that when the molar ratio of CdS and CeO2 was 1:1, the nanocomposites exhibited the best photocatalytic desulfurization activity, reaching 92% in 3 h. Meanwhile, transient photocurrent (PT) measurement, photoluminescence (PL) spectra and electrochemical impedance spectroscopy (EIS) measurement indicated that the modification of CeO2 nanorods by CdS nanoparticles could significantly inhibit the recombination of photogenerated electrons and holes. In addition, the possible mechanism of photocatalytic oxidation desulfurization of the nanocomposites was proposed. This study may provide an effective CeO2-based photocatalyst for photocatalytic desulfurization applications.
Visible-Light-Driven CO2 Reduction into Methanol Utilizing Sol-Gel-Prepared CeO2-Coupled Bi2O3 Nanocomposite Heterojunctions
Authors: Mostafa Mohamed Mokhtar Mohamed , Shawky Ahmed , Zaman Sharif Fakhruz , Narasimharao Katabathini , Abdel Salam Mohamed , Alshehri Abdulmohsen Ali , Khdary Nezar H. , Al-Faifi Sulaiman , Chowdhury Abhishek Dutta
Published: November 2022

Carbon dioxide (CO2) photoreduction into renewable fuels over semiconductor photocatalysts has emerged as a green and sustainable alternative for energy production. Consequently, tremendous efforts are being performed to develop robust and sustainable photocatalysts. Therefore, visible-light active nanocomposite photocatalysts composed of 5.0–20.0 wt.% bismuth oxide (Bi2O3) and cerium oxide (CeO2) were synthesized by a sol-gel-based process. The prepared nanocomposites were evaluated for the promoted photocatalytic reduction of CO2 into methanol (CH3OH). Various characterizations of the obtained photocatalysts exposed an outstanding development of crystalline structure, morphology, and surface texture due to the presence of Bi2O3. Moreover, the absorbance of light in the visible regime was improved with enhanced charge separation, as revealed by the exploration of optical response, photoluminescence, and photocurrent measurements. The overall bandgap calculations revealed a reduction to 2.75 eV for 15% Bi2O3/CeO2 compared to 2.93 eV for pure CeO2. Moreover, the adjusted 2.8 g L−1 dose of 15% Bi2O3/CeO2 selectively produced 1300 μmol g−1 CH3OH after 9 h of visible light irradiation. This photocatalyst also exhibits bearable reusability five times. The improved progression of 15% Bi2O3/CeO2 is denoted by significant charge separation as well as enhanced mobility. This study suggests the application of metal oxide-based heterojunctions for renewable fuel production under visible light.
Sustainable Aromatic Production from Catalytic Fast Pyrolysis of 2-Methylfuran over Metal-Modified ZSM-5
Authors: Xia Shengpeng , Wang Chenyang , Chen Yu , Kang Shunshun , Zhao Kun , Zheng Anqing , Zhao Zengli , Li Haibin
Published: November 2022

The catalytic fast pyrolysis (CFP) of bio-derived furans offers a promising approach for sustainable aromatic production. ZSM-5 modified by different metal species (Zn, Mo, Fe, and Ga) was employed in the CFP of bio-derived furans for enhancing aromatic production. The effects of metal species, metal loadings, and the weight hourly space velocity (WHSV) on the product distributions from the CFP of 2-methylfuran (MF) were systemically investigated. It is found that the introduction of Zn, Mo, Fe, and Ga on ZSM-5 significantly increases the MF conversion and aromatic yields. The maximum MF conversions of 75.49 and 69.03% are obtained, respectively, by Fe-ZSM-5 and Ga-ZSM-5, which boost the aromatic yield by 34.5 and 42.7% compared to ZSM-5. The optimal loading of Fe on ZSM-5 is 2%. Additionally, the highest aromatic yield of 40.03% is achieved by 2%Fe-ZSM-5 at a WHSV of 2 h−1. The catalyst characterization demonstrates that the synergistic effect of Brønsted and Lewis acid sites in Fe-ZSM-5 is responsible for achieving the efficient aromatization of MF. The key to designing improved zeolite catalysts for MF aromatization is the introduction of large numbers of new Lewis acid sites without a significant loss of Brønsted acid sites in ZSM-5. These findings can provide guidelines for the rational design of better zeolite catalysts used in the CFP of biomass and its derived furans.
A Sweet Warning: Mucin-Type O-Glycans in Cancer
Authors: Zhang Yuhan , Sun Lingbo , Lei Changda , Li Wenyan , Han Jiaqi , Zhang Jing , Zhang Yuecheng
Published: November 2022

Glycosylation is a common post-translational modification process of proteins. Mucin-type O-glycosylation is an O-glycosylation that starts from protein serine/threonine residues. Normally, it is involved in the normal development and differentiation of cells and tissues, abnormal glycosylation can lead to a variety of diseases, especially cancer. This paper reviews the normal biosynthesis of mucin-type O-glycans and their role in the maintenance of body health, followed by the mechanisms of abnormal mucin-type O-glycosylation in the development of diseases, especially tumors, including the effects of Tn, STn, T antigen, and different glycosyltransferases, with special emphasis on their role in the development of gastric cancer. Finally, tumor immunotherapy targeting mucin-type O-glycans was discussed.
Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels
Authors: Song Lairong , Li Xiaojie , Xu Xiaoying , Huo Xulei , Zheng Yi , Wang Xiaomin , Li Da , Zhang Junting , Wang Ke , Wang Liang , Wu Zhen
Published: November 2022

Colorectal neoplasia differentially expressed (CRNDE) is an oncogenic long noncoding RNA (lncRNA) overexpressed in diverse malignancies. Here, we comprehensively analyze the prognostic value and molecular function of CRNDE in glioma. Bulk RNA-sequencing data from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA), and single-cell RNA-sequencing data from the Tumor Immune Single-Cell Hub (TISCH) were analyzed. Kaplan–Meier survival analysis was applied to verify the prognostic value of CRNDE. Then, a nomogram based on multivariate Cox regression was established for individualized survival prediction. Subsequently, the expression characteristic and biological function of CRNDE were analyzed at the single-cell level. Lastly, the effects of CRNDE on the proliferation and invasion of glioma cell were explored in vitro. We discovered that CRNDE was a powerful marker for risk stratification of glioma patients. Regardless of the status of IDH and 1p/19q, CRNDE could effectively stratify patients’ prognosis. The nomogram that incorporated the CRNDE expression was proved to be a reliable tool for survival prediction. In addition, epithelial–mesenchymal transition may be the most important biological process regulated by CRNDE, which was identified at both the bulk and single-cell levels. Moreover, CRNDE knockdown significantly inhibited the proliferation and invasion of glioma cell. Overall, CRNDE is a vital oncogene and may be a valuable supplement to improve the clinical stratification of glioma.
Mutation of Tyrosine Sites in the Human Alpha-Synuclein Gene Induces Neurotoxicity in Transgenic Mice with Soluble Alpha-Synuclein Oligomer Formation
Authors: Lassen Louise Berkhoudt , Thomsen Maj Schneider , Basso Elisa , Füchtbauer Ernst-Martin , Füchtbauer Annette , Outeiro Tiago Fleming , Jensen Poul Henning , Moos Torben
Published: November 2022

Overexpression of α-synuclein with tyrosine mutated to phenylalanine at position 125 leads to a severe phenotype with motor impairment and neuropathology in Drosophila. Here, we hypothesized that tyrosine mutations would similarly lead to impaired motor performance with neuropathology in a rodent model. In transgenic mice (ASO), tyrosines at positions 125, 133, and 136 in human α-synuclein were mutated to phenylalanine and cloned into a Thy1.2 expression vector, which was used to create transgenic mouse lines on a mixed genetic background TgN(Thy-1-SNCA-YF)4Emfu (YF). The YF mice had a decreased lifespan and displayed a dramatic motor phenotype with paralysis of both hind- and forelegs. Post-translational modification of α-synuclein due to phosphorylation of serine 129 is often seen in inclusions in the brains of patients with α-synucleinopathies. We observed a slight but significant increase in phosphorylation of serine 129 in the cytosol in YF mice compared to age-matched human α-synuclein transgenic mice (ASO). Conversely, significantly decreased phosphorylation of serine 129 was seen in synaptosomes of YF mice that also contained higher amounts of soluble oligomers. YF mice deposited full-length α-synuclein aggregates in neurons widespread in the CNS with the main occurrence in the forebrain structures of the cerebral cortex, the basal ganglia, and limbic structures. Full-length α-synuclein labeling was also prominent in many nuclear regions of the brain stem, deep cerebellar nuclei, and cerebellar cortex. The study shows that the substitution of tyrosines to phenylalanine in α-synuclein at positions 125, 133, and 136 leads to severe toxicity in vivo. An insignificant change upon tyrosine substitution suggests that the phosphorylation of serine 129 is not the cause of the toxicity.
Functional Role of STIM-1 and Orai1 in Human Microvascular Aging
Authors: El Assar Mariam , García-Rojo Esther , Sevilleja-Ortiz Alejandro , Sánchez-Ferrer Alberto , Fernández Argentina , García-Gómez Borja , Romero-Otero Javier , Rodríguez-Mañas Leocadio , Angulo Javier
Published: November 2022

The impact of aging on vascular function is heterogeneous depending on the vascular territories. Calcium regulation plays a key role in vascular function and has been implicated in aging-related hypercontractility of corpus cavernosum. We aimed to evaluate stromal interaction molecule (STIM)/Orai system involvement in aging-related vascular alterations in the human macro and microvasculature. Aortae specimens and mesenteric arteries (MA), obtained from 45 organ donors, were functionally evaluated in organ chambers and wire myographs. Subjects were divided into groups either younger or older than 65-years old. The expressions of STIM-1, Orai1, and Orai3 were determined by immunofluorescence in the aorta and MA, and by Western blot in the aorta homogenates. The inhibition of STIM/Orai with YM-58483 (20 μM) reversed adrenergic hypercontractility in MA from older subjects but did not modify aging-related hypercontractility in the aortic strips. Aging was related to an increased expression of Orai1 in human aorta, while Orai1 and STIM-1 were upregulated in MA. STIM-1 and Orai1 protein expressions were inversely correlated to endothelial function in MA. Circulating levels of Orai1 were correlated with the inflammatory factor TNF-α and with the endothelial dysfunction marker asymmetric dimethylarginine. Aging is associated with an increased expression of the STIM/Orai system in human vessels with functional relevance only in the microvascular territory, suggesting its role in aging-related microvascular dysfunction.