bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Partial Pressure, Conversion, and Temperature on the Oxidation Reaction Kinetics of Cu2O to CuO in Thermochemical Energy Storage
Authors: Setoodeh Jahromy Saman , Birkelbach Felix , Jordan Christian , Huber Clemens , Harasek Michael , Werner Andreas , Winter Franz
Published: February 2019

Metal oxides are promising potential candidates for thermochemical energy storage in concentrated solar power plants. In particular, the Cu2O/CuO system is suitable because of its high energy density, applied temperature interval, and reduced cost compared to the CoO/Co3O4 system. In heterogenous gas-solid reactions, the pressure affects the kinetics significantly. To quantify this effect for oxidation of Cu2O to CuO, isothermal runs between 800 °C and 930 °C at different oxygen partial pressures (0.1, 0.2, 0.5, and 1.0 bar) were conducted with thermogravimetric analysis (TGA). Defined fractions of CuO samples (1–100 µm) were analyzed with X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) analysis, and scanning electron microscopy (SEM) analysis. The kinetic analyses were performed with extended non-parametric kinetics (NPK), which is applied for the first time to consider the pressure term in the general kinetic equation in addition to the conversion and the temperature term. The results show how the oxygen partial pressure impacts the kinetics and how reparameterization of the pressure term affects the kinetic analysis of the oxidation reaction of Cu2O to CuO. The best conversion model is a two-dimensional Avrami-Erofeev model with an activation energy of 233 kJ/mol. The kinetic models for conversion, temperature, and pressure presented in this work provide one of the most important requirements for reactor designs.
Investigation of the Pitch Load of Large-Scale Wind Turbines Using Field SCADA Data
Authors: Zhang Fan , Dai Juchuan , Liu Deshun , Li Linxing , Long Xin
Published: February 2019

Variable pitch technology is an indispensable key technology of large-scale wind turbines. The reliable pitch mechanism is the basic guarantee for achieving variable pitch. At present, the main problem with the design and maintenance of the variable pitch mechanism is that the pitch load is not clearly known. This paper focuses on obtaining pitch load characteristics through extracting SCADA (Supervisory Control and Data Acquisition) data. Here, the pitch load refers to the resistance moment to be overcome when the wind turbine blade is rotated on its own axis. From the data collected by the SCADA system, although the edgewise moment and the flapwise moment cannot be obtained, the pitch torque (load) can be extracted indirectly. This provides data support for the research. Specifically, the pitch moment is obtained by indirect calculation of the pitch motor current. Then, the effects of the wind speed, rotor speed, hub angle and pitch angle on the pitch load are theoretically analyzed. To obtain more reliable results, data preprocessing algorithms are presented to consider the data filtering range, the elimination of abnormal values and data dispersity. Subsequently, the influence mechanisms of wind speed, rotor speed, hub angle and pitch angle on the pitch load are investigated in detail based on the SCADA data.
A Virtual Inertia-Based Power Feedforward Control Strategy for an Energy Router in a Direct Current Microgrid Application
Authors: Li Yuyang , Sun Qiuye , Wang Danlu , Lin Sen
Published: February 2019

Due to the uncertainty of the power load and the randomness of distributed generations, low-voltage direct current (LVDC) bus voltage fluctuation will greatly affect the safety of an energy router-enabled direct current (DC) microgrid. In this paper, a power feedforward control strategy based on a dual active bridge (DAB) DC/DC converter in an energy router-based DC Microgrid is proposed. Based on this strategy, the LVDC bus voltage is controlled by virtual inertia control of the DC microgrid, instead of by the DAB converter. Thus, two benefits of the proposed strategy can be achieved: the power feedforward control can be realized, to mitigate the voltage fluctuation range of the LVDC bus; and the modulation algorithm in the DAB converter can be simplified. Experimental results verify the correctness and effectiveness of the proposed control method.
Past and Current Trends of Coastal Predatory Fish in the Baltic Sea with a Focus on Perch, Pike, and Pikeperch
Authors: Olsson Jens
Published: February 2019

Coastal predatory fish are of key importance for the provisioning of ecosystem services in the Baltic Sea. Worldwide, however, there has been a general and sharp decline in predatory fish populations, in turn threatening the viability and function of marine ecosystems. On the basis of the literature, the past (data until the 2000s) and current (data until early and mid 2010s) trends in abundance of coastal predatory fish in the Baltic Sea are reviewed in this paper. Potentially important impacting factors behind the temporal development of the populations and measures to strengthen and restore them are also discussed. Available data from coastal fish monitoring programs suggest a stable or increasing abundance of coastal predatory fish as a functional group and for the species perch in the majority of areas assessed in the Baltic Sea. For pike and pikeperch, data to support assessments is scarce, but suggest substantial declines in the abundance of both species in most assessed areas. The impacting factors behind these patterns vary between species and areas, but include climate, habitat exploitation, fishing, and species-interactions in the coastal food web. Measures to restore and support coastal predatory fish communities should follow an ecosystem-based approach to management and include efforts to regulate fisheries sectors in combination with habitat protection and restoration.
RNA 2′-O-Methylation (Nm) Modification in Human Diseases
Authors: Dimitrova Dilyana G. , Teysset Laure , Carré Clément
Published: February 2019

Nm (2′-O-methylation) is one of the most common modifications in the RNA world. It has the potential to influence the RNA molecules in multiple ways, such as structure, stability, and interactions, and to play a role in various cellular processes from epigenetic gene regulation, through translation to self versus non-self recognition. Yet, building scientific knowledge on the Nm matter has been hampered for a long time by the challenges in detecting and mapping this modification. Today, with the latest advancements in the area, more and more Nm sites are discovered on RNAs (tRNA, rRNA, mRNA, and small non-coding RNA) and linked to normal or pathological conditions. This review aims to synthesize the Nm-associated human diseases known to date and to tackle potential indirect links to some other biological defects.
Nanoindentation Studies of Plasticity and Dislocation Creep in Halite
Authors: Thom Christopher , Goldsby David
Published: February 2019

Previous deformation experiments on halite have collectively explored different creep mechanisms, including dislocation creep and pressure solution. Here, we use an alternative to conventional uniaxial or triaxial deformation experiments—nanoindentation tests—to measure the hardness and creep behavior of single crystals of halite at room temperature. The hardness tests reveal two key phenomena: (1) strain rate-dependent hardness characterized by a value of the stress exponent of ~25, and (2) an indentation size effect, whereby hardness decreases with increasing size of the indents. Indentation creep tests were performed for hold times ranging from 3600 to 106 s, with a constant load of 100 mN. For hold times longer than 3 × 104 s, a transition from plasticity to power-law creep is observed as the stress decreases during the hold, with the latter characterized by a value of the stress exponent of 4.87 ± 0.91. An existing theoretical analysis allows us to directly compare our indentation creep data with dislocation creep flow laws for halite derived from triaxial experiments on polycrystalline samples. Using this analysis, we show an excellent agreement between our data and the flow laws, with the strain rate at a given stress varying by less than 5% for a commonly used flow law. Our results underscore the utility of using nanoindentation as an alternative to more conventional methods to measure the creep behavior of geological materials.
Addressing the Gaps in Post-Stroke Sexual Activity Rehabilitation: Patient Perspectives
Authors: Prior Sarah , Reeves Nicole , Peterson Gregory , Jaffray Linda , Campbell Steven
Published: February 2019

Sexual dysfunction is common but often under-recognised or neglected after stroke. This study sought to identify the existing methods for providing information and discussion on post-stroke sexual activity, and perceived gaps from the patient perspective. A sample of 1265 participants who had been admitted to any of the four major public hospitals in Tasmania, Australia, with stroke (International Classification of Diseases (ICD-10) group B70) were mailed a survey assessing their experiences with, and opinions about, receipt of post-stroke sexual activity education. One hundred and eighty-three participants (14.5%) responded; of these, 65% were male and the mean age was 69.1 years. The results indicated that, whilst over 30% or participants wanted to receive information related to post-stroke sexual activity, only a small proportion of participants (8.2%) had received this. In terms of the method of receiving this information, participants preferred to receive this from a doctor in a private discussion with or without their partner present. The delivery of post-stroke sexual activity information and education is inconsistent and fails to meet patient needs within major Tasmanian hospitals, highlighting the importance of developing sound, routine, post-stroke education and information processes.
The Provenance of Marbles and Alabasters Used in the Monuments of Hierapolis in Phrygia (Turkey): New Information from a Systematic Review and Integration of Archaeological and Archaeometric Data
Authors: Scardozzi Giuseppe
Published: February 2019

Determining the provenance of the stones used for ancient architectures is very important in order to reconstruct many social and economic questions linked to the life of a city. This paper integrates previous and new archaeometric data about marble and alabaster quarries in south-western Phrygia (Lykos valley), and offers a review of some results of the research activities carried out between the years 2013–2018 and aimed to reconstruct the building stone procurement strategies adopted in the city of Hierapolis in Phrygia across a broad chronological time span from the Hellenistic age to the Byzantine period. The research activities based on a multidisciplinary approach, integrating the archaeological and art-historian study of the monuments, the topographical investigation of the quarries, and the archaeometric characterisation both of extraction sites and marbles and alabasters used in the building sites of the urban area and in the necropolises.
Quantifying Thermal Characteristics of Stormwater through Low Impact Development Systems
Authors: LeBleu Charlene , Dougherty Mark , Rahn Keith , Wright Amy , Bowen Ryan , Wang Rui , Orjuela Jeisson Andrés , Britton Kaylee
Published: February 2019

Urbanization causes alteration of the thermal regime (surface, air, and water) of the environment. Heated stormwater runoff flows into lakes, streams, bays, and estuaries, which potentially increases the base temperature of the surface water. The amount of heat transferred, and the degree of thermal pollution is of great importance to the ecological integrity of receiving waters. This research reports on a controlled laboratory scale test to assess low impact development (LID) stormwater control measure impacts on the thermal characteristics of stormwater runoff. We hypothesize that LID stormwater control measures (SCMs) such as pervious surfaces and rain gardens/bioretention can be used to mitigate the ground level thermal loads from stormwater runoff. Laboratory methods in this study captured and infiltrated simulated stormwater runoff from four infrared heated substrate microcosms (pervious concrete, impervious concrete, permeable concrete pavers, and turf grass), and routed the stormwater through rain garden microcosms. A data logging system with thermistors located on, within, and at exits of the microcosms, recorded resulting stormwater temperature flux. Researchers compared steady state temperatures of the laboratory to previously collected field data and achieved between 30% to 60% higher steady state surface temperatures with indoor than outdoor test sites. This research helps establish baseline data to study heat removal effectiveness of pervious materials when used alone or in combination as a treatment train with other stormwater control measures such as rain gardens/bioretention.
Utility of Geriatric Syndrome Indicators for Predicting Subsequent Health Care Utilization in Older Adults in Taiwan
Authors: Chiu Ching-Ju , Cheng Ya-Yun
Published: February 2019

Background: The predictive utility of both individual and combined indicators of geriatric syndromes on subsequent emergency use and hospitalization is not clear. Methods: Nationally representative data on adults aged 65+ (N = 2345) (with 1148 male, 1197 female) in Taiwan were analyzed. The receiver operating characteristic (ROC) curve examined the diagnostic accuracy of the combined effects of geriatric syndromes on predicting health care utilization in three years. Negative binomial regressions identified the individual effect of each indicator with the control of sociodemographic and baseline health status. Results: The combined indicators of geriatric syndromes predicted future hospitalization of old-old (75+ yrs) diabetes patients, with area under the curve (AUC) = 0.709, 95% confidence interval (CI) = 0.635–0.782, and young-old patients (65–74 yrs) with mild cognitive impairment (AUC = 0.727, 95% CI = 0.610–0.845 for hospitalization and AUC = 0.770, 95% CI = 0.664–0.877 for emergency visits). As for individual indicators, while incontinence was the indicator having the most influence on hospitalization (incidence rate ratio (IRR) = 1.81, 95% CI = 1.21–2.72) and emergency visits (IRR = 1.78, 95% CI = 1.23–2.59) for general older adults (65+), and for old-old emergency visits, especially (IRR = 2.21, 95% CI = 1.39–3.49), falls was the most prominent indicator of hospitalization for young-old (65-74) adults (IRR = 1.61, 95% CI = 1.13–2.28). In addition, pain was another significant indicator for predicting future hospitalization of old-old diabetes patients (IRR = 1.61, 95% CI= 1.07–2.44). Conclusions: Combined indicators of geriatric syndromes effectively predict hospitalization in old-old (75+ yrs) diabetes patients and hospitalization and emergency visits in young-old (65–74 yrs) patients with cognitive impairment. Incontinence, falls, and pain were the most predictive independent geriatric assessment indicators.
Health Benefits of Physical Activity Related to an Urban Riverside Regeneration
Authors: Vert Cristina , Nieuwenhuijsen Mark , Gascon Mireia , Grellier James , Fleming Lora E. , White Mathew P. , Rojas-Rueda David
Published: February 2019

The promotion of physical activity through better urban design is one pathway by which health and well-being improvements can be achieved. This study aimed to quantify health and health-related economic impacts associated with physical activity in an urban riverside park regeneration project in Barcelona, Spain. We used data from Barcelona local authorities and meta-analysis assessing physical activity and health outcomes to develop and apply the “Blue Active Tool”. We estimated park user health impacts in terms of all-cause mortality, morbidity (ischemic heart disease; ischemic stroke; type 2 diabetes; cancers of the colon and breast; and dementia), disability-adjusted life years (DALYs) and health-related economic impacts. We estimated that 5753 adult users visited the riverside park daily and performed different types of physical activity (walking for leisure or to/from work, cycling, and running). Related to the physical activity conducted on the riverside park, we estimated an annual reduction of 7.3 deaths (95% CI: 5.4; 10.2), and 6.2 cases of diseases (95% CI: 2.0; 11.6). This corresponds to 11.9 DALYs (95% CI: 3.4; 20.5) and an annual health-economic impact of 23.4 million euros (95% CI: 17.2 million; 32.8 million). The urban regeneration intervention of this riverside park provides health and health-related economic benefits to the population using the infrastructure.
Perceived Impact of Taiwan’s National Health Insurance Allocation Strategy: Health Professionals’ Perspective
Authors: Owili Patrick Opiyo , Muga Miriam Adoyo , Yang Ya-Ting , Hsu Yi-Hsin Elsa
Published: February 2019

Studies on health care demand have indicated high levels of public satisfaction with Taiwan’s National Health Insurance (NHI). However, the global budget allocation mechanism (GBAM) used by NHI has led to various adjustments in the providers’ way of practice, quality of care, utilization of care, and health expenditure. Studies focusing on the satisfaction of providers with health care supply, however, remain limited. We therefore explored the provider’s perceived impact of the NHI allocation plan. A cross-sectional data of 299 health professionals was collected at Taipei Medical University Hospitals in April 2012. Perceptions and attitudes were assessed using a validated 5-point Likert-type questionnaire before using a structural equation modeling technique to explore the complex interrelationships of the NHI’s perceived impact. The causal path relationships between the latent variables ‘characteristics of NHI’s allocation plan’ and ‘perceived positive effect’ (β = 0.39), ‘perceived positive effect’ and ‘satisfaction of health professionals’ (β = 0.53), and between ‘characteristics of NHI’s allocation plan’ and ‘satisfaction of health professionals’ (β = 0.30) were positively associated; while the path relationships between the latent variables ‘perceived negative effect’ and ‘satisfaction of health professionals’ (β = −0.27) and ‘characteristics of NHI’s allocation plan’ and ‘attitude toward allocation criteria’ (β = −0.22) were negatively associated. These results indicate that providers perceived a positive impact of the NHI allocation strategy. The NHI allocation plan is an important decision-making tool among policy makers since it helps optimize outcomes. Research based on its impact at both horizontal and vertical levels on the supply side may be useful towards understanding Taiwan’s GBAM. Policy-makers should therefore consider understanding the impact of GBAM at both the demand and supply side in adjusting allocation criteria.
Stomatal Conductance Measurement for Toxicity Assessment in Zero-Effluent Constructed Wetlands: Effects of Landfill Leachate on Hydrophytes
Authors: Białowiec Andrzej , Koziel Jacek A. , Manczarski Piotr
Published: February 2019

In this research, we explore for the first time the use of leaf stomatal conductance (gs) for phytotoxicity assessment. Plants respond to stress by regulating transpiration. Transpiration can be correlated with stomatal conductance when the water vapor pressure gradient for transpiration is constant. Thus, our working hypothesis was that the gs measurement could be a useful indicator of the effect of toxic compounds on plants. This lab-scale study aimed to test the measurement of gs as a phytotoxicity indicator. Our model plants were two common hydrophytes used in zero-effluent constructed wetlands for treating landfill leachate. The toxic influence of two types of leachate from old landfills (L1, L2) on common reed (Phragmites australis (Cav.) Trin. ex Steud.) and sweet flag (Acorus calamus L.) was tested. The gs measurements correlated well with plant response to treatments with six solutions (0 to 100%) of landfill leachate. Sweet flag showed higher tolerance to leachate solutions compared to common reed. The estimated lowest effective concentration (LOEC) causing the toxic effect values for these leachates were 3.94% of L1 and 5.76% of L2 in the case of reed, and 8.51% of L1 and 10.44% of L2 in the case of sweet flag. Leachate L1 was more toxic than L2. The leaf stomatal conductance measurement can be conducted in vivo and in the field. The proposed approach provides a useful parameter for indicating plant responses to the presence of toxic factors in the environment.
Exposure Characterization of Haloacetic Acids in Humans for Exposure and Risk Assessment Applications: An Exploratory Study
Authors: Parvez Shahid , Ashby Jeffrey L. , Kimura Susana Y. , Richardson Susan D.
Published: February 2019

Disinfected water is the major source of haloacetic acids (HAAs) in humans, but their inter- and intra-individual variability for exposure and risk assessment applications is under-researched. Thus, we measured HAAs in cross-sectional and longitudinal urine and water specimens from 17 individuals. Five regulated HAAs—mono-, di-, and trichloroacetic acid (MCAA, DCAA, and TCAA) and mono- and dibromoacetic acid (MBAA and DBAA)—and one unregulated HAA—bromochloroacetic acid (BCAA)—were measured. Urinary DCAA, MBAA, DBAA, and BCAA levels were always below the limits of detection (LOD). Measured levels and interindividual variability of urinary MCAA were higher than urinary TCAA. Longitudinal urinary specimens showed MCAA levels peaked in after-shower specimens, while TCAA levels remain unchanged. Correlation between urinary MCAA and TCAA was moderate but statistically significant. The prevalence of MCAA and TCAA in urine suggest they can be considered as biomarkers of HAA. Peak urinary MCAA in post-shower specimens suggest MCAA captures short-term exposure via dermal and/or inhalation, while urinary TCAA captures long-term exposure via ingestion. However, further research is warranted in a large pool of participants to test the reliability of MCAA as exposure biomarker.
Comparison of Different Home/Commercial Washing Strategies for Ten Typical Pesticide Residue Removal Effects in Kumquat, Spinach and Cucumber
Authors: Wu Yangliu , An Quanshun , Li Dong , Wu Jun , Pan Canping
Published: February 2019

Home processing can reduce pesticide residues in agricultural products, and the common forms of treatment include washing, peeling, blanching, and cooking. In this study, the removal effects of tap water, micron calcium solution, alkaline electrolyzed water (AlEW), ozone water, active oxygen, and sodium bicarbonate on 10 typical pesticide residues in kumquat, cucumber, and spinach were investigated. The residue magnitudes were determined by chromatography–tandem mass spectrometry (GC-MS/MS, LC-MS/MS), combined with the QuEChERS pretreatment method. The model tests showed that the results of soaking and greenhouse were close. The removal effects of pesticide residues in kumquat and cucumber washing by alkaline electrolyzed water with a high pH value, micron calcium, and active oxygen solution were better than other washing solutions. The sodium bicarbonate solution, ozone water, and active oxygen solution were more effective in reducing pesticide residues in spinach than others. Active oxygen solution showed a better removal efficiency for the 10 pesticides than other treatments because of its alkalinity and oxidizability. Among the ten pesticides, pyrethroid pesticides had a higher removal rate. Additionally, chlorpyrifos were the most difficult to remove. For the majority of pesticides, the pesticide residue magnitudes showed a gradual reduction when increasing the washing time. The results indicated that alkaline solutions were effective for the reduction of pesticide residues when the washing time was longer than 15 min.
Pathogenic and Non-Pathogenic Microorganisms in the Rapid Alert System for Food and Feed
Authors: Pigłowski Marcin
Published: February 2019

The most frequently notified pathogenic microorganisms in the RASFF in 1980–2017 were Salmonella sp., Listeria, Escherichia and Vibrio, whereas, among the notified non-pathogenic microorganisms were unspecified microorganisms, Enterobacteriaceae, Salmonella sp. and Coliforms. Microorganisms were reported mainly in poultry meat, meat, fish, molluscs, crustaceans, fruits, vegetables, herbs, spices, nuts, milk, cereals (in food) and in feed materials and pet food (in feed). The number of notifications decreased at the turn of 2005 and 2006, but has steadily increased since then. The notification basis were official controls, border controls and company’s checks. Products were notified mainly by Italy, France, United Kingdom, Germany and Netherlands. The reported products originated from Brazil, European Union countries and India, Thailand and Vietnam. The notification types were alerts, information and border rejections. The distribution status was often not specified or distribution on the market was possible. The risk decision was usually not made. Products were re-dispatched, import was not authorised or products were withdrawn from the market, destroyed and recalled from the market. Proper cooperation within the framework of the RASFF can contribute to shaping public health law and reducing outbreaks associated with microorganisms.
The Happy-Productive Worker Model and Beyond: Patterns of Wellbeing and Performance at Work
Authors: Peiró José M. , Kozusznik Malgorzata W. , Rodríguez-Molina Isabel , Tordera Núria
Published: February 2019

According to the happy-productive worker thesis (HPWT), “happy” workers perform better than “less happy” ones. This study aimed to explore the different patterns of relationships between performance and wellbeing, synergistic (i.e., unhappy-unproductive and happy-productive) and antagonistic (i.e., happy-unproductive and unhappy-productive), taking into account different operationalizations of wellbeing (i.e., hedonic vs. eudaimonic) and performance (i.e., self-rated vs. supervisors’ ratings). It also explored different demographic variables as antecedents of these patterns. We applied two-step cluster analysis to the data of 1647 employees. The results indicate four different patterns—happy-productive, unhappy-unproductive, happy-unproductive, and unhappy-productive—when performance is self-assessed, and three when it is assessed by supervisors. On average, over half of the respondents are unhappy-productive or happy-unproductive. We used multidimensional logistic regression to explain cluster membership based on demographic covariates. This study addresses the limitations of the HPWT by including both the hedonic and eudaimonic aspects of wellbeing and considering different dimensions and sources of evaluation. The “antagonistic” patterns identify employees with profiles not explicitly considered by the HPWT.
Progress and Challenges on Entity Alignment of Geographic Knowledge Bases
Authors: Sun Kai , Zhu Yunqiang , Song Jia
Published: February 2019

Geographic knowledge bases (GKBs) with multiple sources and forms are of obvious heterogeneity, which hinders the integration of geographic knowledge. Entity alignment provides an effective way to find correspondences of entities by measuring the multidimensional similarity between entities from different GKBs, thereby overcoming the semantic gap. Thus, many efforts have been made in this field. This paper initially proposes basic definitions and a general framework for the entity alignment of GKBs. Specifically, the state-of-the-art of algorithms of entity alignment of GKBs is reviewed from the three aspects of similarity metrics, similarity combination, and alignment judgement; the evaluation procedure of alignment results is also summarized. On this basis, eight challenges for future studies are identified. There is a lack of methods to assess the qualities of GKBs. The alignment process should be improved by determining the best composition of heterogeneous features, optimizing alignment algorithms, and incorporating background knowledge. Furthermore, a unified infrastructure, techniques for aligning large-scale GKBs, and deep learning-based alignment techniques should be developed. Meanwhile, the generation of benchmark datasets for the entity alignment of GKBs and the applications of this field need to be investigated. The progress of this field will be accelerated by addressing these challenges.
Molecular Mechanisms Underlying Increase in Lysine Content of Waxy Maize through the Introgression of the opaque2 Allele
Authors: Wang Wei , Niu Suzhen , Dai Yi , Zhai Xinmi , Wang Mingchun , Ding Yanqin , Yang Wenpeng , Zhao Degang
Published: February 2019

The opaque2 (o2) mutation in maize is associated with high lysine content in endosperm and good nutritional value. To improve the nutritional quality of waxy maize, the o2 allele was introgressed into the wxwx line using marker-assisted backcrossing selection technology. The lysine content of o2o2wxwx lines was higher than that of the wxwx line. To reveal the mechanism of increasing lysine content through introgression of the o2 in waxy maize, the transcriptome on kernels (18th day after pollination) of the o2o2wxwx and parent lines was analyzed using RNA-sequencing (RNA-Seq). The RNA-Seq analysis revealed 49 differentially expressed genes (DEGs). Functional analysis showed that these DEGs were mostly related to the catalytic activity and metabolic processes. The O2 gene regulated multiple metabolic pathways related to biological processes (BP) and molecular function (MP) during waxy maize endosperm development. In particular, in the o2o2wxwx lines, the two genes that encode the EF-1α and LHT1 were up-regulated, but the gene that encodes sulfur-rich proteins was down-regulated, raising the grain lysine content. These findings are of great importance for understanding the molecular mechanism underlying the lysine content increase due to o2 allele introgression into waxy maize.
Circadian Regulation of Alternative Splicing of Drought-Associated CIPK Genes in Dendrobium catenatum (Orchidaceae)
Authors: Wan Xiao , Zou Long-Hai , Zheng Bao-Qiang , Wang Yan
Published: February 2019

Dendrobium catenatum, an epiphytic and lithophytic species, suffers frequently from perennial shortage of water in the wild. The molecular mechanisms of this orchid’s tolerance to abiotic stress, especially drought, remain largely unknown. It is well-known that CBL-interacting protein kinase (CIPKs) proteins play important roles in plant developmental processes, signal transduction, and responses to abiotic stress. To study the CIPKs’ functions for D. catenatum, we first identified 24 CIPK genes from it. We divided them into three subgroups, with varying intron numbers and protein motifs, based on phylogeny analysis. Expression patterns of CIPK family genes in different tissues and in response to either drought or cold stresses suggested DcaCIPK11 may be associated with signal transduction and energy metabolism. DcaCIPK9, -14, and -16 are predicted to play critical roles during drought treatment specifically. Furthermore, transcript expression abundances of DcaCIPK16 showed polar opposites during day and night. Whether under drought treatment or not, DcaCIPK16 tended to emphatically express transcript1 during the day and transcript3 at night. This implied that expression of the transcripts might be regulated by circadian rhythm. qRT-PCR analysis also indicated that DcaCIPK3, -8, and -20 were strongly influenced by circadian rhythmicity. In contrast with previous studies, for the first time to our knowledge, our study revealed that the major CIPK gene transcript expressed was not always the same and was affected by the biological clock, providing a different perspective on alternative splicing preference.
Astrocytes in Flavivirus Infections
Authors: Potokar Maja , Jorgačevski Jernej , Zorec Robert
Published: February 2019

Virus infections of the central nervous system (CNS) can manifest in various forms of inflammation, including that of the brain (encephalitis) and spinal cord (myelitis), all of which may have long-lasting deleterious consequences. Although the knowledge of how different viruses affect neural cells is increasing, understanding of the mechanisms by which cells respond to neurotropic viruses remains fragmented. Several virus types have the ability to infect neural tissue, and astrocytes, an abundant and heterogeneous neuroglial cell type and a key element providing CNS homeostasis, are one of the first CNS cell types to get infected. Astrocytes are morphologically closely aligned with neuronal synapses, blood vessels, and ventricle cavities, and thereby have the capacity to functionally interact with neurons and endothelial cells. In this review, we focus on the responses of astrocytes to infection by neurotropic flaviviruses, including tick-borne encephalitis virus (TBEV), Zika virus (ZIKV), West Nile virus (WNV), and Japanese encephalitis virus (JEV), which have all been confirmed to infect astrocytes and cause multiple CNS defects. Understanding these mechanisms may help design new strategies to better contain and mitigate virus- and astrocyte-dependent neuroinflammation.
Quantifying BRCA1 and BRCA2 mRNA Isoform Expression Levels in Single Cells
Authors: Lattimore Vanessa L. , Pearson John F. , Morley-Bunker Arthur E. , Investigators kConFab , Spurdle Amanda B. , Robinson Bridget A. , Currie Margaret J. , Walker Logan C.
Published: February 2019

BRCA1 and BRCA2 spliceogenic variants are often associated with an elevated risk of breast and ovarian cancers. Analyses of BRCA1 and BRCA2 splicing patterns have traditionally used technologies that sample a population of cells but do not account for the variation that may be present between individual cells. This novel proof of concept study utilises RNA in situ hybridisation to measure the absolute expression of BRCA1 and BRCA2 mRNA splicing events in single lymphoblastoid cells containing known spliceogenic variants (BRCA1c.671-2 A>G or BRCA2c.7988 A>T). We observed a large proportion of cells (>42%) in each sample that did not express mRNA for the targeted gene. Increased levels (average mRNA molecules per cell) of BRCA2 ∆17_18 were observed in the cells containing the known spliceogenic variant BRCA2c.7988 A>T, but cells containing BRCA1c.671-2 A>G were not found to express significantly increased levels of BRCA1 ∆11, as had been shown previously. Instead, we show for each variant carrier sample that a higher proportion of cells expressed the targeted splicing event compared to control cells. These results indicate that BRCA1/2 mRNA is expressed stochastically, suggesting that previously reported results using RT-PCR may have been influenced by the number of cells with BRCA1/2 mRNA expression and may not represent an elevation of constitutive mRNA expression. Detection of mRNA expression in single cells allows for a more comprehensive understanding of how spliceogenic variants influence the expression of mRNA isoforms. However, further research is required to assess the utility of this technology to measure the expression of predicted spliceogenic BRCA1 and BRCA2 variants in a diagnostic setting.
Unraveling the Molecular Mechanism of Selective Antimicrobial Activity of 2(5H)-Furanone Derivative against Staphylococcus aureus
Authors: Sharafutdinov Irshad S. , Pavlova Anna S. , Akhatova Farida S. , Khabibrakhmanova Alsu M. , Rozhina Elvira V. , Romanova Yulia J. , Fakhrullin Rawil , Lodochnikova Olga A. , Kurbangalieva Almira R. , Bogachev Mikhail I. , Kayumov Airat R.
Published: February 2019

Staphylococcus aureus causes various infectious diseases, from skin impetigo to life-threatening bacteremia and sepsis, thus appearing an important target for antimicrobial therapeutics. In turn, the rapid development of antibiotic resistance and biofilm formation makes it extremely robust against treatment. Here, we unravel the molecular mechanism of the antimicrobial activity of the recently unveiled F105 consisting of three pharmacophores: chlorinated 2(5H)-furanone, sulfone, and l-menthol moieties. F105 demonstrates highly selective activity against Gram-positive bacteria and biofilm-embedded S. aureus and exhibits low risk of resistance development. We show explicitly that the fluorescent analogue of F105 rapidly penetrates into Gram-positive bacteria independently of their cell integrity and viability and accumulates there. By contrast, Gram-negative bacteria remain impermeable and, therefore, insusceptible to F105. Apparently, in bacterial cells, F105 induces reactive oxygen species (ROS) formation and nonspecifically interacts with a number of proteins, including ROS-utilizing ones. Using native and 2D PAGE, we confirm that F105 changes the charge of some proteins by either oxidation or direct interaction with them. Therefore, it seems justified to conclude that being simultaneously a ROS inducer and damaging proteins responsible for ROS utilization, F105 impairs the cellular anti-ROS defense representing a prospective ROS-inducing antibacterial agent.
Levels of Circulating Fibroblast Growth Factor 23 (FGF23) and Prognosis in Cancer Patients with Bone Metastases
Authors: Mansinho André , Ferreira Arlindo R. , Casimiro Sandra , Alho Irina , Vendrell Inês , Costa Ana Lúcia , Sousa Rita , Abreu Catarina , Pulido Catarina , Macedo Daniela , Pacheco Teresa R. , Correia Lurdes , Costa Luís
Published: February 2019

The fibroblast growth factor (FGF) signaling pathway plays a key role in tumorigenesis and is recognized as a potential therapeutic target. In this study, the authors aimed to assess the impact of serum FGF23 levels in the prognosis of patients with cancer and bone metastases from solid tumors. A cohort of 112 patients with cancer and metastatic bone disease were treated with bone-targeted agents (BTA). Serum baseline FGF23 was quantified by ELISA and dichotomized in FGF23high and FGF23low groups. Additionally, the association between FGF23 and overall survival (OS) and time to skeletal-related events (TTSRE) was investigated. Baseline characteristics were balanced between groups, except for the median urinary N-terminal telopeptide (uNTX) level. After a median follow-up of 26.0 months, a median OS of 34.4 and 12.2 months was found in the FGF23low and FGF23high groups, respectively (multivariate HR 0.18, 95% CI 0.07–0.44, p = 0.001; univariate HR 0.27, p = 0.001). Additionally, TTSRE was significantly longer for patients with FGF23low (13.0 vs. 2.0 months, p = 0.04). Overall, this study found that patients with FGF23low at baseline had longer OS and TTSRE. Further studies are warranted to define its role as a prognostic biomarker and in the use of drugs targeting the FGF axis.
Novel Targeted Anti-Tumor Nanoparticles Developed from Folic Acid-Modified 2-Deoxyglucose
Authors: Jin Shaoming , Du Zhongyao , Guo Huiyuan , Zhang Hao , Ren Fazheng , Wang Pengjie
Published: February 2019

The glucose analog, 2-deoxyglucose (2-DG), specifically inhibits glycolysis of cancer cells and interferes with the growth of cancer cells. However, the excellent water solubility of 2-DG makes it difficult to be concentrated in tumor cells. In this study, a targeted nano-pharmacosome was developed with folic acid-modified 2-DG (FA-2-DG) by using amino ethanol as a cleavable linker. FA-2-DG was able to self-assemble, forming nano-particles with diameters of 10–30 nm. The biological effects were evaluated with cell viability assays and flow cytometry analysis. Compared with a physical mixture of folic acid and 2-DG, FA-2-DG clearly reduced cell viability and resulted in cell cycle arrest. A computational study involving docking simulation suggested that FA-2-DG can dock into the same receptor as folic acid, thus confirming that the structural modification did not affect the targeting performance. The results indicated that the nano-pharmacosome consisting of FA-2-DG can be used for targeting in a nano-drug delivery system.
TRAF6 Silencing Attenuates Multiple Myeloma Cell Adhesion to Bone Marrow Stromal Cells
Authors: Morgan Jonathan J. , McAvera Roisin M. , Crawford Lisa J.
Published: February 2019

The bone marrow (BM) microenvironment plays an important role in supporting proliferation, survival and drug resistance of Multiple Myeloma (MM) cells. MM cells adhere to bone marrow stromal cells leading to the activation of tumour-promoting signaling pathways. Activation of the NFκB pathway, in particular, is central to the pathogenesis of MM. Tumour necrosis factor receptor-associated factor 6 (TRAF6) is a key mediator of NFκB activation and has previously been highlighted as a potential therapeutic target in MM. Here, we demonstrate that adherence of MM cell lines to stromal cells results in a reciprocal increase in TRAF6 expression. Knockdown of TRAF6 expression attenuates the ability of MM cells to bind to stromal cells and this is associated with a decrease in NFκB-induced expression of the adhesion molecules ICAM1 and VCAM1. Finally, we show that knockdown of TRAF6 sensitizes MM cells to treatment with bortezomib when co-cultured with stromal cells. Inhibiting TRAF6 represents a promising strategy to target MM cells in the BM microenvironment.
The Role of Osteoprotegerin and Its Ligands in Vascular Function
Authors: Rochette Luc , Meloux Alexandre , Rigal Eve , Zeller Marianne , Cottin Yves , Vergely Catherine
Published: February 2019

The superfamily of tumor necrosis factor (TNF) receptors includes osteoprotegerin (OPG) and its ligands, which are receptor activators of nuclear factor kappa-B ligand (RANKL) and TNF-related apoptosis-inducing ligand (TRAIL). The OPG/RANKL/RANK system plays an active role in pathological angiogenesis and inflammation as well as cell survival. It has been demonstrated that there is crosstalk between endothelial cells and osteoblasts during osteogenesis, thus establishing a connection between angiogenesis and osteogenesis. This OPG/RANKL/RANK/TRAIL system acts on specific cell surface receptors, which are then able to transmit their signals to other intracellular components and modify gene expression. Cytokine production and activation of their receptors induce mechanisms to recruit monocytes and neutrophils as well as endothelial cells. Data support the role of an increased OPG/RANKL ratio as a possible marker of progression of endothelial dysfunction in metabolic disorders in relationship with inflammatory marker levels. We review the role of the OPG/RANKL/RANK triad in vascular function as well as molecular mechanisms related to the etiology of vascular diseases. The potential therapeutic strategies may be very promising in the future.
Indoxyl Sulfate Induces Apoptosis through Oxidative Stress and Mitogen-Activated Protein Kinase Signaling Pathway Inhibition in Human Astrocytes
Authors: Lin Yi-Ting , Wu Ping-Hsun , Tsai Yi-Chun , Hsu Ya-Ling , Wang Han Ying , Kuo Mei-Chuan , Kuo Po-Lin , Hwang Shang-Jyh
Published: February 2019

Uremic toxins accumulated in chronic kidney disease (CKD) increases the risk of cognitive impairment. Indoxyl sulfate (IS) is a well-known protein-bound uremic toxin that is correlated with several systemic diseases, but no studies on human brain cells are available. We investigated the effect of IS on primary human astrocytes through next-generation sequencing and cell experiment confirmation to explore the mechanism of IS-associated brain damage. Total RNAs extracted from IS-treated and control astrocytes were evaluated by performing functional and pathway enrichment analysis. The toxicities of IS in the astrocytes were investigated in terms of cell viability through flow cytometry; the signal pathway was then investigated through immunoblotting. IS stimulated the release of reactive oxygen species, increased nuclear factor (erythroid-derived 2)-like 2 levels, and reduced mitochondrial membrane potential. IS triggered astrocyte apoptosis by inhibiting the mitogen-activated protein kinase (MAPK) pathway, including extracellular-signal-regulated kinase (ERK), MAPK/ERK kinase, c-Jun N-terminal kinase, and p38. The decreased ERK phosphorylation was mediated by the upregulated dual-specificity phosphatase 1, 5, 8, and 16. In conclusion, IS can induce neurotoxicity in patients with CKD and the pathogenesis involves cell apoptosis through oxidative stress induction and MAPK pathway inhibition in human astrocytes.
Effects of a Short-Term Cycling Interval Session and Active Recovery on Non-Linear Dynamics of Cardiac Autonomic Activity in Endurance Trained Cyclists
Authors: Gronwald Thomas , Hoos Olaf , Hottenrott Kuno
Published: February 2019

Measurement of the non-linear dynamics of physiologic variability in a heart rate time series (HRV) provides new opportunities to monitor cardiac autonomic activity during exercise and recovery periods. Using the Detrended Fluctuation Analysis (DFA) technique to assess correlation properties, the present study examines the influence of exercise intensity and recovery on total variability and complexity in the non-linear dynamics of HRV. Sixteen well-trained cyclists performed interval sessions with active recovery periods. During exercise, heart rate (HR) and beat-to-beat (RR)-intervals were recorded continuously. HRV time domain measurements and fractal correlation properties were analyzed using the short-term scaling exponent alpha1 of DFA. Lactate (La) levels and the rate of perceived exertion (RPE) were also recorded at regular time intervals. HR, La, and RPE showed increased values during the interval blocks (p < 0.05). In contrast, meanRR and DFA-alpha1 showed decreased values during the interval blocks (p < 0.05). Also, DFA-alpha1 increased to the level in the warm-up periods during active recovery (p < 0.05) and remained unchanged until the end of active recovery (p = 1.000). The present data verify a decrease in the overall variability, as well as a reduction in the complexity of the RR-interval-fluctuations, owing to increased organismic demands. The acute increase in DFA-alpha1 following intensity-based training stimuli in active recovery may be interpreted as a systematic reorganization of the organism with increased correlation properties in cardiac autonomic activity in endurance trained cyclists.
Effects of Caloric Restriction with Protein Supplementation on Plasma Protein Profiles in Middle-Aged Women with Metabolic Syndrome—A Preliminary Open Study
Authors: Chang Chia-Yu , Tung Yu-Tang , Lin Yen-Kuang , Liao Chen-Chung , Chiu Ching-Feng , Tung Te-Hsuan , Shabrina Amalina , Huang Shih-Yi
Published: February 2019

Background: Clinical studies have demonstrated that higher protein intake based on caloric restriction (CR) alleviates metabolic abnormalities. However, no study has examined the effects of plasma protein profiles on caloric restriction with protein supplementation (CRPS) in metabolic syndrome (MetS). Therefore, using a proteomic perspective, this pilot study investigated whether CRPS ameliorated metabolic abnormalities associated with MetS in middle-aged women. Methods: Plasma samples of middle-aged women with MetS in CR (n = 7) and CRPS (n = 6) groups for a 12-week intervention were obtained and their protein profiles were analysed. Briefly, blood samples from qualified participants were drawn before and after the dietary treatment. Anthropometric, clinical, and biochemical variables were measured and correlated with plasma proteomics. Results: In results, we found that body mass index, total body fat, and fasting blood glucose decreased significantly after the interventions but were not different between the CR and CRPS groups. After liquid chromatography–tandem mass spectrometry analysis, the relative plasma levels of alpha-2-macroglobulin (A2M), C4b-binding protein alpha chain (C4BPA), complement C1r subcomponent-like protein (C1RL), complement component C6 (C6), complement component C8 gamma chain (C8G), and vitamin K-dependent protein S (PROS) were significantly different between the CRPS and CR groups. These proteins are involved in inflammation, the immune system, and coagulation responses. Moreover, blood low-density lipoprotein cholesterol levels were significantly and positively correlated with C6 plasma levels in both groups. Conclusions: These findings suggest that CRPS improves inflammatory responses in middle-aged women with MetS. Specific plasma protein expression (i.e., A2M, C4BPA, C1RL, C6, C8G, and PROS) associated with the complement system was highly correlated with fasting blood glucose (FBG), blood lipids (BLs), and body fat.