bluuebunny/crossref_metadata_2025 huggingface dataset:
Legal Problems of Determination of Limits of Balance Sheet Attribution and Operational Responsibility
Authors:   Postgraduate Student of the Novosibirsk State University of Economics and Management (NSUEM), Krasnikova Lyubov A.
Published: August 2024

In this article, the author examines the emerging disagreements between the organization of water utilities and subscribers on determining the boundaries of balance ownership and operational responsibility for external water supply and sanitation networks of capital construction facilities technologically connected to centralized water supply and sanitation systems. The author analyzes the normative legal acts of the Soviet period that establish requirements for the construction of capital construction facilities and define the concept of “balance ownership” of water supply and sanitation networks between subscribers and utilities. In the course of the conducted research, the author revealed contradictions between the current legislation establishing the concept of “boundaries of balance ownership” and the physical nature of the process of technological connection of capital construction facilities to centralized water supply and sanitation systems. The author proposes to define the concept of “boundaries of balance ownership” precisely taking into account the principle of single connection and to establish a boundary at the point of connection to centralized water supply and sanitation systems defined in the technical conditions or the connection agreement.
A CRIMINAL OFFENSE AND PUBLIC DANGER GO HAND IN HAND
Authors:   Justice of the Peace of the Judicial Subdistrict of the Erzinsky Kozhuun of the Republic of Tuva, Ondar Otchugash V.
Published: August 2024

The article analyzes the doctrinal definition of a criminal offense serving as the basis for singling out of four groups of scientists with various approaches to its understanding. The author draws a conclusion on the insignificant character and low public danger of a criminal offense.
ON THE LEGAL NATURE OF THE GOOD FAITH PRINCIPLE
Authors:   Lecturer of the S.N. Lebedev Department of Private International and Civil Law, Dvalishvili Lika Z.
Published: August 2024

This article studies the legal nature of the good faith principle, develops a unique approach unknown to the legal doctrine and practice that views good faith as a projection of the human conscience on the legal field with the required amendments. Considering the mentioned approach, the author expresses and justifies the opinion that good faith should to a great extent be based on values underlying the human conscience, primarily, the idea of justice and protection of the weakest party to legal relations. Taking into account that conscience is something an individual is born with rather than what is given by the society, the socially acknowledged views of what's due manifested by public morale and the generally accepted business practice acting as a criterion of good faith behavior cannot be viewed as ideally compliant with the human conscience requirements; in view of that, the author suggests raising the good faith behavior standard. Since the moral value of an act is determined by the desire to do good and show love, evaluation of its predicted results, while good faith is a projection of the human conscience on the legal field, the evaluation of behavior of persons at law as to good faith should be substantially based on the true motives of such behavior. In the final part, the author concludes that good faith is a civil law principle that manifests moral origins, the idea of justice and protection of the weakest party to legal relations, stipulates reasonable consideration of the subjective side of legal behavior of parties to regulated legal relations.
THE CONCEPT AND LEGAL ESSENCE OF INTERNATIONAL COOPERATION IN THE SPHERE OF CRIMINAL PROCEEDINGS
Authors:   Associate Professor of the Department of Criminal Law, Criminal Procedure and Criminalistics of the Moscow State Institute of International Relations, Klevtsov Kirill K.
Published: August 2024

The article gives general characteristics of the concept and legal essence of international cooperation in the sphere of criminal proceedings, in particular, describes the correlation between the reviewed concept and international police (criminal intelligence and surveillance) cooperation. Based on the results of the study, the author draws the respective conclusions based on the analysis of provisions of international treaties, national laws and some experience of foreign states.
CLASSROOM ACTION RESEARCH TO IMPROVE STUDENTS' NUMERACY SKILLS ON INTEGER USING MANIK-MANIK MEDIA
Authors: Ndapa Deda Yohanis , Hijriani Lailin , Trifosa Alnabe Ayu
Published: August 2024

This study aims to improve students' numeracy literacy skills on integer operation material using Manik-Manik media. The method used is Classroom Action Research (CAR). Research is carried out in two cycles, and each process consists of three meetings, with stages: planning/preparation of actions, implementation of activities, observation, and reflection. Data collection techniques using observation techniques (teachers and students), learning outcomes tests, and documentation. Based on the results of the research on 25 students, it is known that there was increasing in student learning outcomes from before the action, Cycle I and Cycle II. This is evidenced by the post-test value that was carried out in cycle I, which showed an average value of 59.4, and then the increase in cycle II with an average value of 90.8. The data analysis techniques used are data analysis of observations of teachers and students and analysis of test result data in the form of classical completeness. Based on the research on integer operation material, it was concluded that learning by using Manik-Manik can improve students' mathematics learning outcomes. Thus, using  Manik-Manik media can improve students' numeracy literacy skills on integer operation materials.
Keywords:
Integer, Manik-Manik  Media, Numeration Literacy, CAR.
EFFECTIVENESS OF COMICS LEARNING MEDIA TO IMPROVE THE TOLERANCE ATTITUDE OF JUNIOR HIGH SCHOOL LEVEL STUDENTS
Authors: Anggraeni Sugiarto Apprillia , Kurniawan Dandi , Ridwan Muhammad , Sudarwati Ninik
Published: August 2024

The motto "Bhinneka Tunggal Ika" is the basis for diversity in Indonesia. However, the level of tolerance is a concern, especially regarding the disapproval of exclusive groups. This research emphasizes the importance of the value of tolerance, particularly among the nation's younger generation. Education is considered a platform for building tolerance by including subject matter that explores understanding of cultural, religious, and ethnic diversity. This research focuses on the use of comics as learning media, which can capture students' attention through a combination of pictures and stories, helping them understand the values of tolerance more deeply. This Classroom Action Research (PTK) aims to measure the effectiveness of using comics to increase understanding of the value of tolerance among students of class VIII-D at SMPN 3 Jombang. The results show that the percentage obtained by students in the pre-cycle only up to 6%, then in cycle I a percentage of 59% was achieved using an average of 76.25. In cycle II, there was a significant increase, reaching 94% with an average score of 84.66. In conclusion, comic learning media is effective in the learning process because it can enhance students' knowledge and understanding of the importance of tolerance.
Turnover Factors for Certified Registered Nurse Anesthetists
Authors: Lambert Michael C , Ward Robyn C , Riddle J Dru
Published: August 2024

Objective: The objective of this scoping review is to understand the extent and type of evidence related to occupational turnover factors for certified registered nurse anesthetists (CRNA) in the United States.       
Introduction: Demand for anesthesia services is increasing dramatically. CRNAs provide a significant number of anesthetics each year and are crucial to accessible anesthesia in the U.S. Understanding occupational turnover factors for CRNAs is vital to providing cost effective, reliable anesthesia services for Americans.
Inclusion criteria: Studies relevant to CRNA turnover in the U.S. were included for this review. Due to differences in education, scope of practice, and practice environment, studies involving nurse anesthetists outside the U.S. were excluded. Studies with mixed provider populations including physicians, registered nurses, other advanced practice providers, and students were also excluded.
Methods: MEDLINE Complete, Embase, and Google Scholar were sources searched for relevant literature. Studies were only limited to those published in English. No publication date limitations were used. Titles and abstracts were reviewed, with potential papers retrieved for independent review by the authors. Data extraction was conducted independently by the authors using the Joanna Briggs Institute (JBI) data extraction instrument template. Themes identified after data extraction are described in narrative format.
Results: After review of the literature, five studies were included for data extraction. All the studies employed electronic or mailed questionnaires. Common factors associated with occupational turnover were compensation, retirement, job satisfaction, and burnout.
Conclusions: Findings suggest that occupational turnover factors are multifactorial and include broad concepts such as job satisfaction, burnout, and organizational climate.
Protamine Reaction in Cardiovascular Surgery
Authors: Walker Gage
Published: August 2024

Coronary artery bypass grafting is the most performed cardiac surgery throughout the world today. The United States alone performs over 200,00 of these procedures every year. Protamine sulfate is administered to neutralize heparin given during these cases. Known hemodynamic effects are associated with protamine and on rare occasions, true anaphylaxis. A 10.7% chance exists of a protamine reaction occurring after its administration and five different risk factors that increase that chance are an allergy to fish, use of NPH insulins, previous vasectomy, previous exposure to protamine, and rapid administration of protamine. Treatment includes fluid resuscitation, administration of vasopressors, intra-aortic balloon pump, and also methylene blue but this has not been studied. A heparin removal device as an alternative to protamine shows promise.
Budownictwo zrównoważone w mieście – oszczędność i racjonalizacja
Authors: Tokarski Daniel
Published: August 2024

Opracowanie ma na celu systematyzację wiedzy na temat racjonalizacji budownictwa w Polsce. W oparciu o przegląd literatury autor dokonuje identyfikacji wytycznych oraz wskazuje ogół przepisów dotyczących projektowania budynków energooszczędnych, koncentrując się na uwarunkowaniach prawnych charakterystyki energetycznej, termomodernizacji oraz audycie energetycznym budynków. Niniejszy tekst przybliża teorie budownictwa niskoenergetycznego, zrównoważonego i ekologicznego, ukazuje, że odpowiednio zaplanowane i przeprowadzone inwestycje mogą spełniać wymogi prawne i mieć obniżone koszty eksploatacji, bez konieczności rezygnowania z komfortu użytkowania. Publikacja łączy w sobie interdyscyplinarne podejście w obszarach nauk inżynieryjno-technicznych oraz nauk społecznych.
Acquisition of –Ing in English by Multilingual Adult Speakers in Germany and the US
Authors: Efeoglu Gulumser
Published: August 2024

The number of the multilingual speakers all over the world has been increasing steadily, which calls for closer analyses of multilingualism as a phenomenon. The current study aims at investigating multilingual speakers’ spoken and written English productions in formal and informal contexts within the boundaries of social context of migration. As for the purposes of the study, data coming from four groups in Germany and the US (32 participants and 24 speakers from RUEG corpus, 56 people in total) via data collection tools such as Language Situations (Wiese 2018), Linguistic Background Questionnaire and c-tests were analyzed both qualitatively and quantitatively. The results were divergent. The comparison of -ing use only revealed that the acquisition of the progressive marker in L3? English differs in Germany and the US while -ing use in total Communication Units (henceforth CU) ratio signaled some cross-linguistic effects. However, there was no difference in within group comparisons obscuring multilingual-monolingual dichotomy within both Germany and the US. Also, task modality and registers were found to have a prominent effect on L3 patterns of English progressive morpheme -ing.
Examining Pre-service Mathematics Teachers' Purposes of Using ChatGPT in Lesson Plan Development
Authors: Pişkin Tunç Mutlu
Published: August 2024

This study aimed to examine pre-service middle school mathematics teachers' purposes of using ChatGPT in lesson plan development. Phenomenology, one of the qualitative research designs, was applied in this study. A total of 56 third-grade pre-service middle school mathematics teachers, 43 females and 13 males were selected for the study. A questionnaire with open-ended questions was employed to gather data. First, pre-service teachers were asked to select one or more learning outcomes in the mathematics curriculum and prepare a lesson plan to address these outcomes using the ChatGPT program. Then, they completed the questionnaire. Content analysis was utilized in the current study to analyze the data. According to the results of the study, ChatGPT served as a valuable tool for pre-service mathematics teachers during lesson plan development. While not all participants utilized it for every subcategory, ChatGPT played a significant role in various aspects, including determining the subject scope, getting creative and interesting ideas, explaining math concepts, establishing relationships and the course flow, developing learning activities, problems, and evaluation tools, and even reviewing and improving the lesson plan. Moreover, pre-service teachers used ChatGPT to deepen their knowledge of mathematical concepts, learning strategies, and teaching models. However, it is important to remember that ChatGPT should be viewed as a support system, not a replacement for a teacher's knowledge and expertise.
Yoğun Bakımda Pnömoni Tanılı Geriatrik Hastalarda Prognozun Nötrofil-Lenfosit Oranı, Trombosit-Lenfosit Oranı ve Nötrofil-Trombosit Oranı ile İlişkisi Var mıdır?
Authors: Doğancı Melek , Sarıyıldız Pehlivan Merve , Cırık Mustafa Özgür , Eraslan Doğanay Güler , Kaybal Oya , Ülger Gülay , Alagöz Ali , Sazak Hilal , Tahhan Metin
Published: August 2024

ÖZET
Amaç:
Yoğun bakım ünitesinde (YBÜ) özellikle geriatrik hastalarda sistemik inflamasyon ve enfeksiyonun erken dönemde saptanması tedavi başarısını ve mortaliteyi etkilemektedir. Bunun saptanması için günümüzde ucuz, hızlı ve kolay bir şekilde bakılan yöntemler tercih edilmektedir. Bu çalışmada pnömoni nedeni ile yoğun bakım ünitesine kabul edilen geriatrik hastaların nötrofil-lenfosit oranı (NLR), trombosit-lenfosit oranı (PLR) ve nötrofil-trombosit oranı (NPR) değerlerinin prognoz açısından değerlendirilmesi amaçlanmıştır.
Yöntem: 1 Ocak 2017-1 Ocak 2020 tarihleri arasında pnömoni nedeni ile YBÜ’ye yatırılan 65 yaş ve üstü 176 hasta retrospektif olarak incelendi. Akut Fizyoloji Ve Kronik Sağlık Değerlendirmesi-II (APACHE II), Demografik veriler ve Charlson Komorbidite İndeks Skoru (CCIS), YBÜ yatış süresi, Ardışık Organ Yetmezliği Değerlendirme Skoru (SOFA), hastane yatış süresi ve 1 aylık mortalite durumları kaydedildi. YBÜ yatışları sırasında bakılan mutlak nötrofil, lenfosit ve trombosit değerleri ile hesaplanan NLR PLR ve NPR değerlerinin prognoz ve mortalite ile ilişkisi değerlendirildi.
Bulgular: Çalışmamızda NLR (r:0,33; p
Exploring the influence of lecturers’ teaching on student’s entrepreneurial intentions at a South African TVET College
Authors: Malawu N , Waghid Z
Published: August 2024

The purpose of this study was to ascertain the challenges faced by a group of TVET college students in establishing their own entrepreneurial ventures. It further aimed to explore how lecturers’ current pedagogical approaches were or were not cultivating the entrepreneurial intentions of these students. The study employed a qualitative approach informed by an interpretive design. The sample participants included five (n=5) lecturers employed to teach entrepreneurship and business management across different courses, and thirty students (n=30) at a TVET college. The Theory of Planned Behaviour served as a barometer to both guide and frame the interpretation of results. A combined student- and teacher-centered approach was found, according to the views of the participants, to have a positive impact on the development of the participating students’ entrepreneurial intentions. The findings also suggest that using real-life business challenges as part of pedagogy, has the potential to help TVET college students develop entrepreneurial intentions, together with the appropriate skills, values, and attitudes.
Effective Mechanisms for Handling Code of Ethics Violations by Election Organizers
Authors: Laxamanahady Moch. Syafrudin Dwi Sapto , Musthofa Moh Ahza Ali , Laxamana M. Herzegovin
Published: August 2024

Elections are fundamental to democracy, allowing for a leader’s free and fair selection. Electoral justice is essential for upholding election integrity by enforcing ethical standards and ensuring the professionalism of election organizers, thereby maintaining public trust and democratic principles. This research examines mechanisms for addressing violations of the electoral code of ethics, covering processes from reporting and investigation to sanctioning and preventive measures. The study utilizes a legal research methodology, employing statute and conceptual approaches. Findings indicate that the DKPP (Dewan Kehormatan Penyelenggara Pemilu) faces several challenges, including its limited presence with an office only in the capital, sanctions that are not sufficiently deterrent, and a small membership of only seven individuals. To improve effectiveness, DKPP needs to enhance human resource training, leverage information technology for better reporting systems, and boost transparency. The establishment of Regional Examination Teams (TPD) has been introduced to accelerate the handling of ethical violations in regional areas. DKPP aims to uphold the integrity and credibility of election organizers, which is crucial for ensuring fair and democratic elections. Raising public awareness about the importance of the code of ethics is also vital to preventing future violations. Despite implementing sanctions such as warnings and suspensions, the effectiveness of these measures remains in question. Strengthening oversight bodies, legal reforms, community engagement, and inter-agency collaboration are necessary to enforce the code of ethics effectively.
Cryptocurrency Investing: Millennial Decision Making
Authors: Kiruba DR Angelin S. , DR Bharathi R , DR Madhumithaa N
Published: August 2024

Investor perceptions of various investment modes can differ based on factors such as experience, earnings, risk tolerance, liquidity preferences, and so on. Understanding the viewpoints of young investors is essential when assessing cryptocurrency investments. This study evaluates the investment decisions of 103 young investors in the cryptocurrency market, using regression and factor analysis for data analysis. The findings indicate that investors have a fundamental understanding of the risks associated with cryptocurrency investments. However, there is a notable need for enhancing the effective management of these risks. The study affirms the reliability of the measures used, accurately capturing the underlying factors pertinent to cryptocurrency investment
Long COVID Among People With Preexisting Disabilities
Authors: Hall Jean P. , Kurth Noelle K. , McCorkell Lisa , Goddard Kelsey S.
Published: August 2024

Objectives. To document the prevalence of long COVID among a sample of survey respondents with long-term disabilities that existed before 2020 and to compare the prevalence among this group with that among the general population.

Methods. We conducted a cross-sectional, descriptive study using data from the 2022 National Survey on Health and Disability (n = 2262) and comparative data for the general population from the federal Household Pulse Survey (HPS).

Results. The prevalence of long COVID was higher among people with preexisting disabilities than in the general population (40.6% vs 18.9%).

Conclusions. People with preexisting disabilities experienced and continue to experience increased exposure to COVID-19 and barriers to accessing health care, COVID-19 vaccines, and COVID-19 tests. These barriers, combined with long-standing health disparities in this population, may have contributed to the greater prevalence of long COVID among people with disabilities.

Public Health Implications. The needs of people with disabilities must be centered in the response to the COVID-19 pandemic and future pandemics. ( Am J Public Health. 2024;114(11):1261–1264. https://doi.org/10.2105/AJPH.2024.307794 )
The Strategic Role of KLL UMSIDA in Graduating Thousands of Scholars
Authors: Kusuma Kumara Adji , Hidayatulloh
Published: August 2024

The Institution of Amil Zakat Infaq and Sadaqah Muhammadiyah (Lazismu) have a vital role in the economic development of the Ummah (Muslim Society) in Indonesia. In this context, Indonesia state which constitutionally gets the main mandate in Article 34 of the 1945 Constitution to improve the welfare of the people of Indonesia is not so significant in carrying out this function with many poor people who have not been alleviated in terms of economy, health or education. Lazismu then equips with a strategic role in efforts to become a back-up solution for the Indonesian state. As part of the Ummah and nation Lazismu becomes a solution, more specifically in the context of this research the object is the Lazismu Service Office (KLL) Muhammadiyah University of Sidoarjo (UMSIDA) in increasing the level of education of the ummah. Through the Lazismu scholarship program Sang Surya, more than a thousand students of higher education managed to continue and complete their undergraduate education. This scientific work in a descriptive phenomenological way reveals the strategic role of the UMSIDA KLL in graduating scholars in Indonesia. It is hoped that this research can become a benchmark for other campuses to take the same role in graduating scholars through zakat infaq and shadaqah funds.
Concept of Islamic Values in Entrepreneurship Implementation
Authors: Aeni Umdatul , Nurhayati Eka , Zakiyah Ummu , Nisa Zahrotun , Arifin M. Syamsul , Muryadi Muryadi , Rahmawati Imelda Dian
Published: August 2024

This research delves into the concept of entrepreneurship within Islamic principles, viewing it as a form of worship and a means to enhance the economy of Muslims. Employing a library research method, the study explores the Islamic perspective on entrepreneurship and its implications for economic development. It reveals that entrepreneurship, when aligned with Islamic law, is seen as obedience to Allah's commands and is highly recommended. Entrepreneurs, guided by Islamic values, demonstrate piety, integrity, and good service, with their actions driven by intentions to please Allah. The study underscores the significance of ethical entrepreneurship in promoting economic prosperity while adhering to religious principles.Highlights:

Entrepreneurship as worship: Islam sees entrepreneurship as a form of worship, promoting economic improvement through ethical business practices.
Ethical values: Islamic entrepreneurship emphasizes piety, integrity, and good service, reflecting moral principles in business.
Success through adherence: Entrepreneurs following Islamic law seek success by ethical means, driven by intentions to please Allah and contribute positively to society.

Keywords: Entrepreneurship, Value, Islam
Motivation and Constraints of the Ummahaat in Learning Arabic at Mahad Umar bin Khattab Muhammadiyah University Sidoarjo
Authors: Amini Anzalna Rahmah , Hikmah Khizanatul
Published: August 2024

Language is a communication tool to convey one's wishes to someone else around the world with various characteristics. Arabic is the second most widely used international language, in learning it there are several obstacles that most people experience. Among them is the age factor, which affects memory when receiving lessons, health, mood, tuition fees, signals (for those who are online), distance, besides that it is also a factor in dividing time between family, study and work. In order to overcome this, supporting factors such as strong motivation during the learning process are needed. This is experienced by the ummahat Mahad Umar Bin khattab Muhammadiyah University of Sidoarjo, their azzam to be able to return to study at a young age, the underlying motivation for the ummahat in learning Arabic is to understand the Al-Quran and translate it well, as a provision for communication, educating their children, job demands, as a filler in free time, motivating peers, and realizing the ideals of youth to study in college.
Highlights: 

Age challenges: Memory decline, health issues, and time constraints pose hurdles for Arabic learners, especially older individuals.
Motivation: Strong desire to understand the Quran, advance careers, and fulfill personal aspirations drives learners to overcome obstacles in Arabic language acquisition.
Ummahat's role: Mothers from Mahad Umar Bin Khattab Muhammadiyah University display determination to learn Arabic despite challenges, balancing family, education, and personal growth.

Keyword: Motivation, Constrain, Ummahat, Learning Arabic
Description of the Sense of Community in Regional Leadership Members of the East Java Ikatan Pelajar Muhammadiyah
Authors: Rahmawati Axnes Tasya , Mariyati Lely Ika
Published: August 2024

This study investigates the relationship between sense of community and performance among members of the Muhammadiyah Student Association in East Java. Employing a descriptive quantitative research method, data were collected from a population of 65 individuals using saturated sampling. Research instruments, derived from previous studies, demonstrated satisfactory validity and reliability. Results reveal a predominantly high sense of community among members, with 9.20% categorized as very high, 21.50% as high, 43.20% as medium, 20% as low, and 6.10% as very low. These findings underscore the importance of fostering a strong sense of community within groups, as it positively impacts individual and collective performance, thus offering valuable insights for community development and organizational management.

Highlights:

Importance of sense of community: Emphasizes the role of community cohesion in driving individual and collective performance.
Research methodology: Descriptive quantitative approach provides an overview of sense of community among members.
Findings: Majority of members exhibit high to very high sense of community, highlighting strong group cohesion within the Muhammadiyah Student Association in East Java.

Keywords: Community, Sense of Community, Ikatan Pelajar Muhammadiyah
Impact of Marketing Mix on Patient Perception and Loyalty
Authors: Fahmi Shiddiqi , Supardi
Published: August 2024

This study investigates patient perceptions of the marketing mix and its impact on loyalty at Muhammadiyah Probolinggo Mother and Child Hospital. Through quantitative analysis of questionnaire responses, the study explores the relationship between patient perceptions and loyalty, employing observational survey methods. Findings reveal that patient perceptions, particularly of service quality, place, and tariff, significantly influence loyalty. While there's a positive perception of the marketing mix overall, areas like promotion need improvement. The study underscores the importance of understanding patient perceptions to enhance loyalty and suggests strategies for hospital management to improve patient experience and foster loyalty, contributing to healthcare service quality enhancement.
Highlight:

Perception affects loyalty: Quantitative analysis reveals marketing mix influence.
Marketing mix impact: Quality, place, tariff influence patient loyalty.
Management implications: Enhance perception, improve service for increased loyalty.

Keywoard: Patient Perception, Marketing Mix, Loyalty, Muhammadiyah Probolinggo Hospital, Quantitative Analysis
Kesenian Wayang Kulit Sebagai Sarana Publikasi Sejarah dalam Penyebaran Islam di Jawa
Authors: Anjani Fitria Nungki , Fahruddin
Published: August 2024

Wayang is a cultural heritage of the ancestors of the Indonesian people which is estimated to have existed since ±1500 years BC. In Java, shadow puppets are used and utilized to spread the preaching of Islamic teachings. The spread of Islam in Java is inseparable from the role of the Wali Sango in spreading Islamic teachings. Thanks to the saints Islam was able to flourish in the life of society. This study aims to, (1) know the history of shadow puppets, (2) explain the role of wayang kuit as a medium for spreading Islam in Java, (3) explain the spread of Islam in Java, (4), know the role of walisanga in spreading Islam in Java. This type of research uses qualitative descriptive methods. The conclusion that can be drawn is that wayang has been around since before the existence of Hindhu Buddhism, in its development shadow puppets were used for the spread of Islam, especially on the island of Java. In its dissemination, there is a role of walisangas, one of which is Sunan Kalijaga who spread Islamic teachings with the media of Wayang Kulit.
Structural insights into the mechanism of phosphate recognition and transport by human XPR1
Authors: Liu Zhu , Zhang Wenhui , Chen Yanke , Guan Zeyuan , Du Zhangmeng , Cheng Meng , Zhang Jie , Zuo Jiaqi , Cheng Peng , Wang Qiang , Liu Yanjun , Zhang Delin , Yin Ping
Published: August 2024

XPR1 is the only known protein that transports inorganic phosphate (Pi) out of cells, and the function is conserved across species from yeast to mammals1-4. Human XPR1 variants lead to cerebral calcium-phosphate deposition, which are associated with a neurodegenerative disorder known as primary familial brain calcification (PFBC)5. Here, we present the Cryo-EM structure of human XPR1 bound to Pi ions. XPR1 contains 10 transmembrane α-helices, forming an ion channel-like architecture that recognizes and transports Pi ions. Two arginine residues, subject to pathogenic mutation in PFBC families, line the translocation channel and serve to bind Pi ions. Clinically linked mutations of these arginine residues impair the Pi transport activity of XPR1. To track the movement of Pi ions within the translocation channel, we capture a mutant XPR1 in an alternative conformation. It reveals a rearrangement of intrahelical hydrogen bonds between a channel-lining tryptophan and two Pi-binding residues. This rearrangement links Pi recognition and transport, by means of flipping the tryptophan residue to propel Pi through the translocation channel. Our results provide mechanistic understanding of how XPR1 recognizes and transports phosphate ions across cell membrane, and they establish a framework for interpreting disease-related mutations and for the development of future therapeutics.
Virulence Plasmid with IroBCDN Deletion Promoted Cross-regional Transmission of ST11-KL64 Carbapenem-resistant Hypervirulent Klebsiella Pneumoniae in central China
Authors: Hong Han-xu , Huo Bing-Hui , Xu Ying , Xiang Tian-Xin , Wei Dan-Dan , Huang Qi-Sen , Liu Peng , Zhang Wei , Liu Yang
Published: August 2024

Background:Carbapenem-resistant and hypervirulent Klebsiella pneumoniae (CR-hvKP) caused infections of high mortality and brought a serious impact on public health. This study evaluated the transmissibility of CR-hvKP among different regions to provide corresponding advice for cross-regional prevention.
Methods: Clinical K. pneumoniae strains were collected from Jiujiang region and Nanchang region in Jiangxi province from November 2021 to June 2022. We characterized these strains for their genetic relatedness, virulence phenotype, and dissimilarities in virulence plasmid structures using PFGE, Galleria mellonella, Whole-genome sequencing.
Results: Among 609 strains, 45 (7.4%) CR-hvKP were identified, while the strains
isolated from Nanchang and Jiujiang accounted for 10.05% (36/358) and 3.59% (9/251). We observed that ST11-KL64 CR-hvKP had an overwhelming epidemic dominance in these two regions. Significant genetic diversity was identified among all ST11-KL64 CR-hvKP cross-regional transmission between Nanchang and Jiujiang serving as major drivers of clonal clusters. Virulence genes profile revealed that ST11-KL64 CR-hvKP might harbour incomplete pLVPK-like plasmids and primarily evolved from CRKP by acquiring the hypervirulence plasmid. We found the predominance of truncated-IncFIB/IncHI1B type virulence plasmids with a 25kb fragment deletion that encoded iroBCDN clusters.
Conclusion: ST11-KL64 is the most cross-regional prevalent type CR-hvKPs in Jiangxi province, which mainly evolved from CRKPs by acquiring a truncated-IncHI1B/IncFIB virulence plasmid with the deletion of iroBCDN. Stricter surveillance and control measures are urgently needed to prevent the epidemic transmission of ST11-KL64 CR-hvKP.
Faxonius rusticus impact on the biodiversity of benthic macroinvertebrate assemblages in the Conodoguinet Creek
Authors: Carmichael Madeleine
Published: August 2024

The Rusty Crawdad (Faxonius rusticus) is a species invasive to the streams of Pennsylvania, chiefly the Conodoguinet Creek. However, little research has been done to investigate Faxonius rusticus’ impact on the biodiversity of the Conodoguinet’s particular ecosystem. The biodiversity of benthic macroinvertebrates is often used to determine the health of lotic ecosystems and as such was tested in order to analyze the mechanisms through which Faxonius rusticus may be altering the creek’s food web. Although the results exhibited a degree of discordance, data collected via this method suggested that biodiversity of the Conodoguinet was generally of good quality despite the presence of Faxonius rusticus. Thus, it was speculated that Faxonius rusticusmay be beneficial to the Conodoguinet Creek ecosystem due to its predation on another invasive species residing in the Conodoguinet: the Asian clam (Corbicula fluminea). Faxonius rusticus may have been able to uphold the biodiversity of this ecosystem’s benthic macroinvertebrate assemblages by precluding the explosive growth of Corbicula fluminea populations and thus allowing native species to compete. As a result, this study suggests that invasive species, such as Faxonius rusticus, may have complex effects on biodiversity varying by ecosystems’ specific food webs.
The optimal dose of human umbilical-cord mesenchymal stem cells (hUC-MSCs) on hypoxic ischemic brain damage(HIBD) rats via intranasal transplantation
Authors: Yang Mengxue , Zheng Kun , Zhang R , He Guangzhen , Xu Jin , Jiang Hao , Ren La , Zhao Liang , Liao Yan , Fu Zeqin , Liu Wenting , Gu Jiaowei
Published: August 2024

Background: Hypoxic ischemic brain damage(HIBD) cause permanent damage to the central nervous system (CNS), including mental retardation, aphasia, epilepsy, cerebral palsy, and developmental delays. Recently, human umbilical cord mesenchymal stem cells (hUC-MSCs) have emerged as a promising cell therapy in HIBD. The optimal dose of stem cell therapy remains open.
Methods: Previously, we conducted a study to identify that the best route of administration of hUC-MSCs to HIBD rat model is intranasal transplantation.
In this study, we conducted the comparison experiments for the optimal dose of MSC via the best route of administration, intranasal transplantation. Four different doses (0.5*106, 1.0*106, 1.5*106, 2.0*106) of hUC-MSCs were injected to HIBD rats (obtained HIBD at 7th day after birth) on the10th day after birth.
The body weight, hanging wire test and Morris Water Maze test were used on the 28th day of different groups of rats to compare their motor function, memory, and learning ability. The HE staining was used to compare the morphological differences of brain tissues of rats in different groups. Moreover, the immunohistochemistry was adopted to compare the morphology and number of astrocytes and microglia in cortex and CA1, CA3 region of the brain of different groups.
Results: All the four different doses of hUC-MSCs treatment significantly improved their body weight, the motor function, memory, learning ability, the morphology and number of astrocytes and microglia in cortex and CA1, CA3 region of HIBD rats. The 1.5*106 of hUC-MSCs group significantly outperform other three groups (0.5*106, 1.0*106, 2.0*106) on both neurobehavioral motor function improvement and morphological improvement, i.e., reducing the number of astrocytes and microglia in cortex and CA1, CA3 region of HIBD rats. The study suggests that 1.5*106 of hUC-MSCs was the optimal dose for rats who obtained HIBD at 7th day after birth and injected by intranasal transplantation on the 10th day after birth.
Ovarian treatment with Autologous Platelet-Rich Plasma plus autologous Bone Marrow-derived Stem cells. Case series
Authors: Alzogaray Victoria , Orihuela Ana , Mazza Milton , Vernocchi Rita , Guidobono Martha , Buzzi Juan Rodriguez , Novick Wanda
Published: August 2024

Introduction: There are limited treatment options for women with severely diminished ovarian reserve (DOR) who experience repeatedly failed in vitro fertilization (IVF) cycles. Therefore, most patients resort to using donor oocytes. Ovarian rejuvenation is an innovative procedure intended to reactivate the remaining follicles in the ovary to improve ovarian response in assisted reproduction treatments. Case presentation: Using two treatments at the same time, we enhance the effects of intraovarian platelet-rich plasma (PRP) injection and Autologous Bone Marrow-Derived Mesenchymal Stem Cells on ovarian stimulation outcomes in three women referred to a Uruguayan IVF center. Conclusion: Data presented herein suggest that autologous intraovarian PRP infusion plus Stem Cells may restore ovarian function. This may enable the achievement of pregnancy. However, the occurrence of spontaneous pregnancy, clinical pregnancy and live birth reflect a significant change. Longer follow-up may detect a functional recovery with improvement of the ovarian reserve hormone markers.
FTO suppresses cardiac fibrosis after myocardial infarction via m 6 A-mediated epigenetic modification of EPRS
Authors: Wang Jian , Li Yanyan , Deng Lijie , Zha Yafang , Zhang Song
Published: August 2024

Background
Cardiac fibrosis is common in cardiovascular diseases. Previous research has suggested that m6A modification is vital in cardiovascular diseases. However, the effects of m6A demethylase fat mass and obesity protein (FTO) on cardiac fibrosis have not been detected.
Methods
Western blot and Quantitative real-time PCR were performed to detect the expression of FTO in the fibrotic tissue of rats and hypoxia-induced cardiac fibroblasts (CFs). The HIF-1 signal pathway was enriched, and a CUT&Tag assay was performed to explore the effects on FTO. The biological functions of FTO were analyzed in vitro and in vivo. The underlying targets of FTO were selected through RNA-seq with m6A-seq. The following dual luciferase reporter assay and RNA stability assay were conducted to investigate the mechanisms of FTO-mediated m6A regulation.
Results
The expression of FTO was decreased in the fibrotic tissue of rats and hypoxia-induced CFs. The knockdown of HIF-1α, instead of HIF-2α, increased the mRNA level of FTO. HIF-1α could bind to the promoter of FTO and inhibit FTO expression. FTO inhibited collagen synthesis in CFs. According to m6A-seq and RNA-seq, EPRS was selected as the underlying target of FTO-induced m6A regulation. IGF2BP3 recognized and bound to the m6A sites of EPRS mRNA, which improved its stability. EPRS was required for cardiac fibrosis induced by FTO silencing.
Conclusions
HIF-1α induced by MI bound to the FTO promoter and inhibited FTO expression. FTO suppressed collagen synthesis of CFs in cardiac fibrosis via m6A-mediated modification, which provided a new therapeutic strategy for cardiac fibrosis.
Brain 18FDG-PET pattern in cognitively impaired elderly patients with bipolar disorder
Authors: Saleh Nouredine , Blaise Capucine , Daoudi Amina , Queneau Matthieu , Fard Karim , Dumurgier Julien , Munoz-Musat Esteban , Marlinge Emeline , Hugon Jacques , Hourregue Claire , Paquet Claire , Cognat Emmanuel
Published: August 2024

Background
Patients with bipolar disorder (BD) are at increased risk of dementia. The underlying mechanisms are debated. This study aims to investigate cerebral glucose metabolism in cognitively impaired elderly BD using FDG-PET imaging, to elucidate potential underlying mechanisms and improve diagnostic accuracy.
Methods
We conducted a retrospective analysis of FDG-PET scans from 32 cognitively impaired elderly patients with BD (mean age 70.4 years). These were compared with scans from 35 neurocognitive controls (NCC) and patients diagnosed with Alzheimer’s disease (AD, n = 27), frontotemporal dementia (FTD, n = 26), and dementia with Lewy bodies (DLB, n = 18). Voxel-wise statistical analysis was performed using SPM software, adjusting for age and sex.
Results
No significant cortical hypometabolism was found in BD patients compared to NCC. In contrast, typical patterns of hypometabolism were observed in the AD, FTD, and DLB groups. The findings suggest that late-life cognitive impairment in BD is not due to a single common neurodegenerative process.
Conclusion
The absence of abnormal cortical metabolism in cognitively impaired elderly patients with BD suggests that cognitive impairment in this population may not be driven by a common neurodegenerative pathway. Further studies using other biomarkers are needed to investigate the brain processes involved, which could lead to improved understanding and management of cognitive impairment in BD.
Using pathogen genomics to predict antigenic changes of influenza H3N2 virus
Authors: Wang Lin , Yao Sidan , Pei Yao , Huang Angkana , lefrancq Noemie , Sun Kaiyuan , Du Zhanwei , Luo Wei , Han Alvin , Liu Yong , Goh Rick Siow Mong , Xu Yani , Lee Raphael , Russell Colin , Cauchemez Simon , Maurer-Stroh Sebastian , Luo Tao , Salje Henrik
Published: August 2024

Influenza viruses evolve their antigenic phenotype in response to immune-driven selection, necessitating regular updates to the antigens used in influenza vaccines. Characterising antigenic change of influenza virus currently requires intensive serology-based laboratory work to analyse the movements of viruses in antigenic space. By contrast, pathogen genomics can generate large numbers of sequences in near real-time. However, using genetic sequences to predict antigenic changes remains challenging due to the complex relationship between genetic and antigenic changes. Here we develop a deep learning approach to predict the antigenic distance between influenza H3N2 viruses using 83,145 hemagglutinin (HA) sequences. We first train an elementary protein language model to encode the HA sequences, and then couple the trained language model with a deep regression model to realise the sequence-based prediction. Our method provides stable and accurate prediction on the antigenic distance between H3N2 viruses using their HA sequences. This facilitates the prediction of antigenic movements of viruses in each influenza season using their sequence data. Our sequence-based method will enable real-time prediction of influenza antigenic changes and support vaccine strain selection.
Integration of transcriptomic and metabolomic data reveals key regulatory defense pathways associated with high temperature stress in cucumber (Cucumis sativus)
Authors: Yuan Yong , Ma Xiao , Li Chuang , Zhong Xitong , Li Yuyan , Zhao Jianyu , Zhang Xiaolan , Zhou Zhaoyang
Published: August 2024

High temperature stress seriously affects the quality and yield of vegetable crops, especially cucumber. However, the metabolic dynamics and underlying regulatory network of cucumber response to high temperature stress remain poorly studied. In this study, we identified a heat-tolerant cucumber Gy14 and a heat-sensitive cucumber 32X. RNA-seq analysis of Gy14 and 32X under high temperature stress showed that some differentially expressed genes (DEGs) were related to the biosynthesis of secondary metabolites. Metabolomic analysis revealed that there were more phenylpropanoids and their downstream derivatives in Gy14 compared to 32X under HS_R2 condition (2 normal days recovery after heat). Integrated analysis of transcriptome and metabolome revealed that the upregulation of genes plays a pivotal role in flavonoid biosynthesis. Moreover, high temperature stress significantly induced the expression of the gibberellin biosynthesis genes and exogenous application of GA3 alleviated the damage high temperature to cucumber seedlings. Together, these findings provided new insights into the transcriptome response and metabolomic reprogramming of cucumber against high temperature stress.
Deep learning PM 2.5 hybrid prediction model based on clustering- secondary decomposition strategy
Authors: Zeng Tao , Liu Yahui , Liu Ruru , Shi Jinli , luo Tao , Xi Yunyun , Zhao Shuo , Chen Chunpeng , Pan Guangrui , Zhou Yuming , Xu Liping
Published: August 2024

Accurate prediction of PM2.5 concentration is important for pollution control, public health and ecological protection. To this end, this paper proposes a deep learning hybrid prediction model based on clustering and secondary decomposition, aiming to achieve accurate prediction of PM2.5 concentration. The model utilizes the complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN) to decompose the PM2.5 sequences into multiple intrinsic modal function components (IMFs), and clusters and re-fuses the sub-sequences with similar complexity by permutation entropy (PE) and K-means clustering. For the fused high-frequency sequences a secondary decomposition is performed using the whale optimization algorithm (WOA) optimized variational modal decomposition (VMD). Finally, prediction is performed using the two basic frameworks combined with the long and short-term memory neural network (LSTM). Experiments show that this proposed model exhibits good stability and generalization ability. It does not only make accurate predicts in the short term, but also captures the trends in the long-term prediction. There is a significant performance improvement over the four deep learning baseline models. Further comparisons with existing models outperform the current state-of-the-art models.