bluuebunny/crossref_metadata_2025 huggingface dataset:
A Combined Proteomic and Transcriptomic Signature Is Predictive of Response to Anti-PD-1 Treatment: A Retrospective Study in Metastatic Melanoma Patients
Authors: Mallardo Domenico , Fordellone Mario , White Andrew , Vowinckel Jakob , Bailey Michael , Sparano Francesca , Sorrentino Antonio , Mallardo Mario , Facchini Bianca Arianna , De Filippi Rosaria , Ferrara Gerardo , Vanella Vito , Beeler Kristina , Chiodini Paolo , Cesano Alessandra , Warren Sarah , Ascierto Paolo A.
Published: August 2024

Resistance biomarkers are needed to identify patients with advanced melanoma obtaining a response to ICI treatment and developing resistance later. We searched a combination of molecular signatures of response to ICIs in patients with metastatic melanoma. In a retrospective study on patients with metastatic melanoma treated with an anti-PD-1 agent carried out at Istituto Nazionale Tumori—IRCCS—Fondazione “G. Pascale”, Naples, Italy. We integrated a whole proteome profiling of metastatic tissue with targeted transcriptomics. To assess the prognosis of patients according to groups of low and high risk, we used PFS and OS as outcomes. To identify the proteins and mRNAs gene signatures associated with the patient’s response groups, the discriminant analysis for sparse data performed via partial least squares procedure was performed. Tissue samples from 22 patients were analyzed. A combined protein and gene signature associated with poorer response to ICI immunotherapy in terms of PFS and OS was identified. The PFS and OS Kaplan–Meier curves were significantly better for patients with high expression of the protein signature compared to patients with low expression of the protein signature and who were high-risk (Protein: HR = 0.023, 95% CI: 0.003–0.213; p < 0.0001. Gene: HR = 0.053, 95% CI: 0.011–0.260; p < 0.0001). The Kaplan–Meier curves showed that patients with low-risk gene signatures had better PFS (HR = 0 0.221, 95% CI: 0.071–0.68; p = 0.007) and OS (HR = 0.186, 95% CI: 0.05–0.695; p = 0.005). The proteomic and transcriptomic combined analysis was significantly associated with the outcomes of the anti-PD-1 treatment with a better predictive value compared to a single signature. All the patients with low expression of protein and gene signatures had progression within 6 months of treatment (median PFS = 3 months, 95% CI: 2–3), with a significant difference vs. the low-risk group (median PFS = not reached; p < 0.0001), and significantly poorer survival (OS = 9 months, 95% CI: 5–9) compared to patients with high expression of protein and gene signatures (median OS = not reached; p < 0.0001). We propose a combined proteomic and transcriptomic signature, including genes involved in pro-tumorigenic pathways, thereby identifying patients with reduced probability of response to immunotherapy with ICIs for metastatic melanoma.
Artificial Intelligence and Machine Learning Technologies for Personalized Nutrition: A Review
Authors: Tsolakidis Dimitris , Gymnopoulos Lazaros P. , Dimitropoulos Kosmas
Published: August 2024

Modern lifestyle trends, such as sedentary behaviour and unhealthy diets, have been associated with obesity, a major health challenge increasing the risk of multiple pathologies. This has prompted many to reassess their routines and seek expert guidance on healthy living. In the digital era, users quickly turn to mobile apps for support. These apps monitor various aspects of daily life, such as physical activity and calorie intake; collect extensive user data; and apply modern data-driven technologies, including artificial intelligence (AI) and machine learning (ML), to provide personalised diet and lifestyle recommendations. This work examines the state of the art in data-driven technologies for personalised nutrition, including relevant data collection technologies, and explores the research challenges in this field. A literature review, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline, was conducted using three databases, covering studies from 2021 to 2024, resulting in 67 final studies. The data are presented in separate subsections for recommendation systems (43 works) and data collection technologies (17 works), with a discussion section identifying research challenges. The findings indicate that the fields of data-driven innovation and personalised nutrition are predominately amalgamated in the use of recommender systems.
The Smoky Impact of Nicotinic Acetylcholine Receptors on Testicular Function
Authors: Barbagallo Federica , Assenza Maria Rita , Torrisi Filippo , Buonacquisto Alessandra , Pallotti Francesco
Published: August 2024

Smoking habits (from classic cigarettes to e-cigarettes and heated tobacco) are a relatively common finding in the medical histories of couples referred to fertility centers. Tobacco smoke and e-cigarettes may deliver many substances with known harmful effects on both general and reproductive health, including nicotine. Nicotinic Acetylcholine receptors (nAChRs) form a heterogeneous family of ion channels that are differently expressed in different tissues. According to the homomeric or heteromeric combination of at least five different subunits (named from α to ε), they have peculiar pharmacological and biophysical properties. nAChRs respond to the neurotransmitter acetylcholine, which influences a number of physiological functions not restricted to neurons and plays an important role in the structure and function of non-neuronal tissues such as the testis. nAChRs are also the target of Nicotine, the active element responsible for tobacco addiction. This review summarizes recent findings on the involvement of nAChRs in testicular physiology, highlighting the effects of nicotine exposure observed in animal studies and clinical settings. We will discuss the latest data on fertility outcomes and the implications for understanding nAChR functions in reproductive health.
Psychological Well-Being of Children with Asthma and Their Parents
Authors: Ferraro Valentina Agnese , Spaggiari Silvia , Zanconato Stefania , Traversaro Letizia , Carraro Silvia , Di Riso Daniela
Published: August 2024

Background/Objectives: The literature reports that emotional disorders in asthmatic children and their parents may affect asthma control. This research explores the baseline psychological well-being of asthmatic children and their mothers’ and fathers’ psychological functioning, focusing on the influence of the parents’ anxiety and separation anxiety on children’s asthma. Methods: In this cross-sectional study, we enrolled children with non-severe asthma and their parents, and a group of healthy children and their parents. The asthmatic children underwent anamnestic investigation, including asthma control and spirometry. Then, both the asthmatic and control triads filled a survey assessing their psychological functioning. Results: Among the 40 asthmatic children enrolled (8–18 years old), most had good clinical control maintained with GINA (Global Initiative for Asthma) therapy step 1–2 (14 patients, 35%) or step 3–4 (26 patients, 65%); 12 patients (30%) had at least one steroid course in the year before enrollment. They exhibited normal psychological adjustment but elevated levels of general (U = 179,500; p < 0.001) and state anxiety (U = 170,000; p < 0.001) compared to healthy peers. Additionally, the asthmatic children’s mothers displayed higher levels of separation anxiety compared to the fathers (t = −2.865, p = 0.006). Increased separation anxiety in the mothers corresponded to a history of at least one exacerbation in the previous year. Conclusions: The asthmatic children exhibited normal psychological adjustment with higher general and state anxiety. Also, their mothers showed greater levels of separation anxiety compared to their fathers. Lastly, higher mothers’ separation anxiety corresponded to a history of at least one exacerbation in the previous year. The influence of the parents’ psychological well-being on the children’s asthma control was previously unexplored.
Hyperkalemia Management with Intravenous Insulin in Patients with Reduced Kidney Function
Authors: Alzahrani Maram A. , AlAbdan Numan A. , Alahmari Zainab S. , Alshehri Nouf M. , Alotaibi Lama H. , Almohammed Omar A.
Published: August 2024

Background: Insufficient kidney function increases the risk of hyperkalemia and hypoglycemia, particularly in hemodialysis-dependent patients. Hypoglycemia is a common complication of insulin-based hyperkalemia treatment. This study aims to evaluate the efficacy and safety of hyperkalemia treatment in hemodialysis-dependent and -non-dependent patients and identify risk factors associated with hypoglycemia. Methods: A retrospective observational cohort study was conducted to assess the efficacy and safety of hyperkalemia treatment including patients with reduced kidney function and hyperkalemia treated with intravenous insulin. The decline rate of potassium and glucose levels were compared between hemodialysis-dependent and non-dependent patients. In addition, univariate and multivariable logistic regression analyses were performed to identify risk factors associated with hypoglycemia. Results: A total of 172 patients with hyperkalemia and reduced kidney function were included. The steepest reduction of serum potassium levels happened within the first 6 h after insulin administration, at 1.1 and 0.9 mmol/L for hemodialysis-dependent and non-dependent patients, respectively. The incidence of hypoglycemia was 18%, and no significant difference was found between cohorts. Hemodialysis-dependent patients were more likely to be readmitted within one month with hyperkalemia, while all-cause ICU admission was more likely for non-dependent patients. Older patients, and those who had heart failure or received a second dose of insulin to treat hyperkalemia, were more likely to experience hypoglycemia. Conclusions: Monitoring blood glucose levels following insulin administration is essential given the complexity of patients’ factors associated with hypoglycemia resulting from hyperkalemia treatment in patients with insufficient kidney function.
Cone-Beam Computed Tomography (CBCT) Analysis of Mandibular Condyles’ Diameters in Patient with Juvenile Idiopathic Arthritis and Temporomandibular Joint Affection: A Cross-Sectional Investigation
Authors: Donelli Margherita , Lanteri Valentina , Ugolini Alessandro , Bruni Alessandro , Cressoni Paolo , Abate Andrea , Maspero Cinzia
Published: August 2024

Objectives: The aim of this study was to evaluate through analysis using CBCT the transverse and axial diameters of the mandibular condyles in subjects affected by juvenile idiopathic arthritis (JIA) and compare them with those of healthy subjects. Methods: The study was conducted on CBCT scans from the digital archive of the Department of Biomedical Surgical and Dental Sciences, University of Milan, including patients with JIA and using healthy subjects as controls. Inclusion criteria: aged between 7 and 25 years old at the time of the CBCT examination; Caucasian ethnicity; diagnosis of JIA according to the International League of Associations for Rheumatology (ILAR) criteria documented in patients’ records; TMJ involvement; good quality CBCTs covering our region of interest (ROI), from the glabella to the mandibular inferior border; no previous orthodontic/orthopedic treatment; no history of craniofacial trauma or congenital birth defects involving the craniofacial area. Each CBCT scan underwent examination using 3Diagnosys® software. Since data were normally distributed, parametric tests were used for analysis. The sample was divided into three groups: (1) bilateral JIA subjects, (2) unilateral JIA subjects, and (3) healthy controls. Results: We found a statistically significant reduction (p < 0.0001) in the transverse diameter (TR-Diam) of the affected condyles by an average of 1.7 mm, while the axial diameter (AX-Diam) again showed a slight reduction, on average by 0.1 mm, with a non-statistically significant value. Another comparison was made between the unaffected condyles of patients with unilateral JIA and the healthy condyles of the control group. The unilateral unaffected condyles were found to be slightly smaller than those of healthy patients, but without statistically significant differences. We found that in both JIA males and females, the condylar growth tends to stop earlier than the healthy ones. Conclusions: The transverse diameter was found to be more affected than the axial one, causing typical bone resorption and condylar shape. Moreover, we showed that the pathology, in the case of unilateral JIA, does not compromise only the affected condyles; the corresponding condyle that seems to be healthy is actually partially compromised. In addition, we observed that the growth of affected condyles of JIA subjects tends to stop earlier than the condyles of the healthy controls.
Development of the International Federation for Surgery of Obesity and Metabolic Disorders-European Chapter (IFSO-EC) Grade-Based Guidelines on the Surgical Treatment of Obesity Using Multimodal Strategies: Design and Methodological Aspects
Authors: De Luca Maurizio , Belluzzi Amanda , Salminen Paulina , Bueter Marco , Pujol-Rafols Juan , Sakran Nasser , Stier Christine , Taskin Halit Eren , Chiappetta Sonja , Carrano Francesco Maria , Di Lorenzo Nicola , Nienhuijs Simon , Puy Ramón Vilallonga , Stenberg Erik , Emous Marloes , Prager Gerhard , Himpens Jacques , Felsenreich Daniel Moritz , Iannelli Antonio , Parmar Chetan , Copaescu Catalin , Fried Martin , Ruiz-Úcar Elena , Cohen Ricardo V. , Olmi Stefano , Angrisani Luigi , Ribeiro Rui , Bandini Giulia , Scoccimarro Daniele , Ragghianti Benedetta , Monami Matteo
Published: August 2024

Background: The prevalence of obesity is already a worldwide health concern. The development of straightforward guidelines regarding the whole available armamentarium (i.e., medical, endoscopic, and surgical interventions in conjunction with a guidance program) is paramount to offering the best multimodal approach to patients with obesity. Methods: The International Federation for Surgery of Obesity and Metabolic Disorders-European Chapter (IFSO-EC) identified a panel of experts to develop the present guidelines. The panel formulated a series of clinical questions (based on the patient, intervention, comparison, and outcome conceptual framework), which have been voted on and approved. A GRADE methodology will be applied to assess the quality of evidence and formulate recommendations employed to minimize selection and information biases. This approach aims to enhance the reliability and validity of recommendations, promoting greater adherence to the best available evidence. Results: These guidelines are intended for adult patients with a body mass index (BMI) ≥ 30 kg/m2 who are candidates for metabolic bariatric surgery (MBS). The expert panel responsible for developing these guidelines comprised 25 panelists (92% were bariatric surgeons) and 3 evidence reviewers, with an average age of 50.1 ± 10.2 years. The panel focused on 3 key questions regarding the combined use of structured lifestyle interventions, approved obesity management medications, and endoscopic weight loss procedures with MBS. Conclusions: The complexity of obesity as a chronic disease requires a comprehensive knowledge of all the available and feasible therapeutic options. The IFSO-EC society felt the urgent need to develop methodologically valid guidelines to give a full picture and awareness of the possible surgical and non-surgical therapeutic strategies employed with a multimodal approach.
EEG Techniques with Brain Activity Localization, Specifically LORETA, and Its Applicability in Monitoring Schizophrenia
Authors: Zeltser Angelina , Ochneva Aleksandra , Riabinina Daria , Zakurazhnaya Valeria , Tsurina Anna , Golubeva Elizaveta , Berdalin Alexander , Andreyuk Denis , Leonteva Elena , Kostyuk Georgy , Morozova Anna
Published: August 2024

Background/Objectives: Electroencephalography (EEG) is considered a standard but powerful tool for the diagnosis of neurological and psychiatric diseases. With modern imaging techniques such as magnetic resonance imaging (MRI), computed tomography (CT), and magnetoencephalography (MEG), source localization can be improved, especially with low-resolution brain electromagnetic tomography (LORETA). The aim of this review is to explore the variety of modern techniques with emphasis on the efficacy of LORETA in detecting brain activity patterns in schizophrenia. The study’s novelty lies in the comprehensive survey of EEG methods and detailed exploration of LORETA in schizophrenia research. This evaluation aligns with clinical objectives and has been performed for the first time. Methods: The study is split into two sections. Part I examines different EEG methodologies and adjuncts to detail brain activity in deep layers in articles published between 2018 and 2023 in PubMed. Part II focuses on the role of LORETA in investigating structural and functional changes in schizophrenia in studies published between 1999 and 2024 in PubMed. Results: Combining imaging techniques and EEG provides opportunities for mapping brain activity. Using LORETA, studies of schizophrenia have identified hemispheric asymmetry, especially increased activity in the left hemisphere. Cognitive deficits were associated with decreased activity in the dorsolateral prefrontal cortex and other areas. Comparison of the first episode of schizophrenia and a chronic one may help to classify structural change as a cause or as a consequence of the disorder. Antipsychotic drugs such as olanzapine or clozapine showed a change in P300 source density and increased activity in the delta and theta bands. Conclusions: Given the relatively low spatial resolution of LORETA, the method offers benefits such as accessibility, high temporal resolution, and the ability to map depth layers, emphasizing the potential of LORETA in monitoring the progression and treatment response in schizophrenia.
Assessment of OCT and Angio-OCT Parameters in Keratoconus Patients with and without Penetrating Keratoplasty
Authors: Gadamer Anna Maria , Miklaszewski Piotr , Janiszewska-Bil Dominika , Lyssek-Boroń Anita , Dobrowolski Dariusz , Wylęgała Edward , Grabarek Beniamin Oskar , Kiełbasińska Aleksandra , Krysik Katarzyna
Published: August 2024

Background/Objectives: Keratoconus (KC) is a bilateral eye disease characterized by corneal thinning and cone-like deformation, leading to visual impairment. This study evaluated the radial peripapillary capillaries (RPCs) in keratoconus patients with and without penetrating keratoplasty (PKP) using OCT and angio-OCT, comparing the results to a control group. Methods: This retrospective study included 149 eyes, 97 from patients who underwent PKP between January 2018 and February 2023 and 52 from patients who did not undergo PKP. The control group comprised 72 patients (144 eyes) who were healthy volunteers. Measurements included the best corrected visual acuity (BCVA), the intraocular pressure (IOP), slit-lamp biomicroscopy, a fundus examination, and corneal topography, as well as OCT and angio-OCT assessments of the RPCs, retinal nerve fiber layer (RNFL), ganglion cell complex (GCC), and central retinal thickness (CRT). Statistical analyses were performed using Student’s t-test and Pearson’s correlation coefficient. Results: The RNFL was significantly thinner in KC eyes after PKP compared to control eyes (p < 0.001), and the CRT was significantly thicker in KC eyes after PKP compared to control eyes (p = 0.003). However, the GCC was similar across the groups (p = 0.0885). Additionally, RPCs inside the disc were significantly reduced in KC eyes after PKP compared to control eyes (p < 0.0001). A significant positive correlation was found between RPC whole vessel density and RNFL thickness as measured via angio-OCT (r = 0.308, p < 0.0001). Conclusions: This study found that the RPC density inside the disc is significantly reduced in keratoconus patients after penetrating keratoplasty, highlighting RPCs inside the disc as a potential diagnostic tool for further assessment of keratoconus.
Extracorporeal Shock Wave Therapy (eSWT) in Spinal Cord Injury—A Narrative Review
Authors: Opara Józef , Dymarek Robert , Sopel Mirosław , Paprocka-Borowicz Małgorzata
Published: August 2024

Background: Injury of the spinal cord causes motor and sensory dysfunction as well as pathological reflexes, leading to paraplegia or tetraplegia. The sequelae of traumatic spinal cord injury (SCI) are a significant burden and impact on healthcare systems. Despite constant progress in medicine, traumatic SCI still remains irreversible. To date, no satisfying treatment that can enable neuronal regeneration and recovery of function at the damaged level has been found. Hundreds of experiments have been conducted on various possibilities of influencing spinal regeneration; some of them have yielded promising results, but unfortunately, the successes obtained in experimental animals have not translated into humans. Methods: This narrative review article presents the application of extracorporeal shock wave therapy (eSWT) in patients with SCI. The article has been divided into parts: 1) use of extracorporeal shock wave therapy for regeneration of the spinal cord after traumatic spinal cord injury; 2) application of extracorporeal shock wave therapy in spasticity after spinal cord injury. In both cases, the hypotheses of possible mechanisms of action will be described. Results and conclusions: A small number of clinical trials have demonstrated the potential of eSWT to influence the regeneration of the spine, as an innovative, safe, and cost-effective treatment option for patients with SCI. Some reports have shown that eSWT can improve spasticity, walking ability, urological function, quality of life, and independence in daily life.
Three-Dimensionally Printed Elastic Cardiovascular Phantoms for Carotid Angioplasty Training and Personalized Healthcare
Authors: Jędrzejczak Krystian , Antonowicz Arkadiusz , Butruk-Raszeja Beata , Orciuch Wojciech , Wojtas Krzysztof , Piasecki Piotr , Narloch Jerzy , Wierzbicki Marek , Makowski Łukasz
Published: August 2024

Background/Objective: Atherosclerosis is becoming increasingly common in modern society. Owing to the increasing number of complex angioplasty procedures, there is an increasing need for training in cases where the risk of periprocedural complications is high. Methods: A procedure was developed to obtain three-dimensional (3D) models and printing of blood vessels. The mechanical and optical properties of the printed materials were also examined. Angioplasty and stent implantation were tested, and the phantom was compared with the clinical data of patients who underwent interventional treatment. Both laser techniques and cone-beam computed tomography of the phantoms were used for comparison. Results: The printed material exhibited mechanical parameters similar to those of blood vessel walls. The refractive index of 1.473 ± 0.002 and high transparency allowed for non-invasive laser examination of the interior of the print. The printed models behaved similarly to human arteries in vivo, allowing training in treatment procedures and considering vessel deformation during the procedure. Models with stents can be analyzed using laser and cone-beam computed tomography to compare stents from different manufacturers. Conclusions: The developed methodology allows for simple and time-efficient production of personalized vessel phantoms.
Development of a Machine Learning Model for the Classification of Enterobius vermicularis Egg
Authors: Chaibutr Natthanai , Pongpanitanont Pongphan , Laymanivong Sakhone , Thanchomnang Tongjit , Janwan Penchom
Published: August 2024

Enterobius vermicularis (pinworm) infections are a significant global health issue, affecting children predominantly in environments like schools and daycares. Traditional diagnosis using the scotch tape technique involves examining E. vermicularis eggs under a microscope. This method is time-consuming and depends heavily on the examiner’s expertise. To improve this, convolutional neural networks (CNNs) have been used to automate the detection of pinworm eggs from microscopic images. In our study, we enhanced E. vermicularis egg detection using a CNN benchmarked against leading models. We digitized and augmented 40,000 images of E. vermicularis eggs (class 1) and artifacts (class 0) for comprehensive training, using an 80:20 training–validation and a five-fold cross-validation. The proposed CNN model showed limited initial performance but achieved 90.0% accuracy, precision, recall, and F1-score after data augmentation. It also demonstrated improved stability with an ROC-AUC metric increase from 0.77 to 0.97. Despite its smaller file size, our CNN model performed comparably to larger models. Notably, the Xception model achieved 99.0% accuracy, precision, recall, and F1-score. These findings highlight the effectiveness of data augmentation and advanced CNN architectures in improving diagnostic accuracy and efficiency for E. vermicularis infections.
Crossmodal Correspondence Mediates Crossmodal Transfer from Visual to Auditory Stimuli in Category Learning
Authors: Sun Ying , Yao Liansheng , Fu Qiufang
Published: August 2024

This article investigated whether crossmodal correspondence, as a sensory translation phenomenon, can mediate crossmodal transfer from visual to auditory stimuli in category learning and whether multimodal category learning can influence the crossmodal correspondence between auditory and visual stimuli. Experiment 1 showed that the category knowledge acquired from elevation stimuli affected the categorization of pitch stimuli when there were robust crossmodal correspondence effects between elevation and size, indicating that crossmodal transfer occurred between elevation and pitch stimuli. Experiments 2 and 3 revealed that the size category knowledge could not be transferred to the categorization of pitches, but interestingly, size and pitch category learning determined the direction of the pitch-size correspondence, suggesting that the pitch-size correspondence was not stable and could be determined using multimodal category learning. Experiment 4 provided further evidence that there was no crossmodal transfer between size and pitch, due to the absence of a robust pitch-size correspondence. These results demonstrated that crossmodal transfer can occur between audio-visual stimuli with crossmodal correspondence, and multisensory category learning can change the corresponding relationship between audio-visual stimuli. These findings suggest that crossmodal transfer and crossmodal correspondence share similar abstract representations, which can be mediated by semantic content such as category labels.
Emerging Roles of Exocyst Complex in Fungi: A Review
Authors: Zuriegat Qussai , Abubakar Yakubu Saddeeq , Wang Zonghua , Chen Meilian , Zhang Jun
Published: August 2024

The exocyst complex, an evolutionarily conserved octameric protein assembly, plays a central role in the targeted binding and fusion of vesicles at the plasma membrane. In fungal cells, this transport system is essential for polarized growth, morphogenesis, cell wall maintenance and virulence. Recent advances have greatly improved our understanding of the role and regulation of the exocyst complex in fungi. This review synthesizes these developments and focuses on the intricate interplay between the exocyst complex, specific fungal cargos and regulatory proteins. Insights into thestructure of the exocyst and its functional dynamics have revealed new dimensions of its architecture and its interactions with the cellular environment. Furthermore, the regulation of exocyst activity involves complex signaling pathways and interactions with cytoskeletal elements that are crucial for its role in vesicle trafficking. By exploring these emerging themes, this review provides a comprehensive overview of the multifaceted functions of the exocyst complex in fungal biology. Understanding these mechanisms offers potential avenues for novel therapeutic strategies against fungal pathogens and insights into the general principles of vesicle trafficking in eukaryotic cells. The review therefore highlights the importance of the exocyst complex in maintaining cellular functions and its broader implications in fungal pathogenicity and cell biology.
Insulin Resistance in Long COVID-19 Syndrome
Authors: Man Dana Emilia , Andor Minodora , Buda Valentina , Kundnani Nilima Rajpal , Duda-Seiman Daniel Marius , Craciun Laura Maria , Neagu Marioara Nicula , Carlogea Iulia-Stefania , Dragan Simona-Ruxanda
Published: August 2024

Background: The COVID-19 pandemic has caused severe health issues worldwide and contributed to huge financial losses. Key comorbidities linked to an increased risk of severe COVID-19 and higher mortality rates include cardio-metabolic disorders such as type 1 and type 2 diabetes mellitus (T1DM and T2DM), atherosclerotic cardiovascular disease, chronic kidney disease, hypertension, heart failure, and obesity. The persistence of symptoms even after the acute phase is over is termed long COVID-19 syndrome. This study aimed to evaluate the relationship between long COVID-19 syndrome and the development of insulin resistance in previously non-diabetic patients. Methods: A prospective observational study was performed on 143 non-diabetic patients who had tested positive for SARS-CoV-2 infection by a PCR test and were hospitalized in our hospital between January 2020 and December 2022. The clinical and para-clinical data at 0, 4, and 12 months of hospital admission for post-COVID-19 infection follow-up was collected and labeled as t0, t4, and t12. Blood glucose, insulin, and C-peptide levels were measured at the beginning and further at 2, 5, 10, and 30 min after the intravenous arginine stimulation test. Similarly, BMI was calculated, and hs-CRP and ESR levels were noted. The results obtained were statistically analyzed. Results: More than one-third (30.7%) of the included patients developed long COVID-19 syndrome. It was found that 75% of patients with long COVID-19 hospitalized in our clinic developed diabetes within a year of acute infection with COVID-19; therefore, it can be said that the presence of long COVID-19 is a major risk for an altered metabolic status, which can cause diabetes. When comparing the glycemia levels (106 mg/dL) with the BMI at t0, t4, and t12 time intervals, the p-values were found to be 0.214, 0.042, and 0.058, respectively. Almost 62% of the patients having BMI > 30 kg/m2 were found to have an increase in blood glucose levels at 1 year. Similarly, insulin resistance was noted during this interval. A negative correlation of 0.40 for hsCRP and 0.38 for ESR was noted when compared with acute infection with COVID-19. Conclusions: The association between long COVID-19 and insulin resistance highlights the varied and widespread impacts of SARS-CoV-2 infection. Addressing the complexities of long COVID-19 requires a holistic strategy that encompasses both respiratory and metabolic considerations, which is crucial for enhancing the well-being of those enduring this persistent condition.
Real-World Efficacy of Intravitreal Faricimab for Diabetic Macular Edema: A Systematic Review
Authors: Nasimi Safiullah , Nasimi Nasratullah , Grauslund Jakob , Vergmann Anna Stage , Subhi Yousif
Published: August 2024

Background: Diabetic macular edema (DME) is a prevalent exudative maculopathy, and anti-vascular endothelial growth factor (anti-VEGF) therapy is the first-line choice for treatment. Faricimab, a novel anti-VEGF and anti-angiopoietin-2 bispecific agent, has recently been approved for the treatment of DME. In this study, we systematically reviewed the real-world evidence of the efficacy of faricimab for the treatment of DME. Methods: We searched 11 databases for eligible studies. Study selection and data extraction were made independently by two authors in duplicate. Eligible studies were reviewed qualitatively. Results: We identified 10 eligible studies that summarized data from a total of 6054 eyes with a mean follow-up of between 55 days and 12 months. Five studies reported outcomes in a population of both treatment-naïve and previously treated eyes, and five studies reported outcomes exclusively in relation to eyes that were previously treated. Faricimab improved the best-corrected visual acuity and macular thickness. The extension of the treatment interval was possible in 61–81% of treatment-naïve eyes and 36–78% of previously treated eyes. Conclusions: Faricimab for DME yields clinical outcomes similar to those known from previous anti-VEGF treatments but with extended treatment intervals, thus lowering the burden of therapy for patients. Long-term real-world studies are warranted.
Urolithin A Ameliorates the TGF Beta-Dependent Impairment of Podocytes Exposed to High Glucose
Authors: Lewko Barbara , Wodzińska Milena , Daca Agnieszka , Płoska Agata , Obremska Katarzyna , Kalinowski Leszek
Published: August 2024

Increased activity of transforming growth factor-beta (TGF-β) is a key factor mediating kidney impairment in diabetes. Glomerular podocytes, the crucial component of the renal filter, are a direct target of TGF-β action, resulting in irreversible cell loss and progression of chronic kidney disease (CKD). Urolithin A (UA) is a member of the family of polyphenol metabolites produced by gut microbiota from ellagitannins and ellagic acid-rich foods. The broad spectrum of biological activities of UA makes it a promising candidate for the treatment of podocyte disorders. In this in vitro study, we investigated whether UA influences the changes exerted in podocytes by TGF-β and high glucose. Following a 7-day incubation in normal (NG, 5.5 mM) or high (HG, 25 mM) glucose, the cells were treated with UA and/or TGF-β1 for 24 h. HG and TGF-β1, each independent and in concert reduced expression of nephrin, increased podocyte motility, and up-regulated expression of b3 integrin and fibronectin. These typical-for-epithelial-to-mesenchymal transition (EMT) effects were inhibited by UA in both HG and NG conditions. UA also reduced the typically elevated HG expression of TGF-β receptors and activation of the TGF-β signal transducer Smad2. Our results indicate that in podocytes cultured in conditions mimicking the diabetic milieu, UA inhibits and reverses changes underlying podocytopenia in diabetic kidneys. Hence, UA should be considered as a potential therapeutic agent in podocytopathies.
Deciphering Motorists’ Perceptions of Scenic Road Visual Landscapes: Integrating Binocular Simulation and Image Segmentation
Authors: Bai Zhaocheng , Ji Rui , Qi Jun
Published: August 2024

Traditional scenic road visual landscape assessment methods struggle to quantify drivers’ subjective visual perceptions. This study aims to develop a new method to decipher Scenic Road Visual Landscape Evaluation (SRVLE) of motorists’ visual field, reconciling the longstanding subjectivity–objectivity dichotomy in landscape quality research. By adopting binocular visual simulation and image segmentation, this paper conceptualizes a novel “non-scale semantic differential approach” to quantify landscape qualities across the dimensions of naturalness–artificiality (NA), diversity–coherence (DC), and openness–deepness (OD), constructing a three-dimensional visual landscape quality evaluation system. Taking the Nujiang Beautiful Road in Yunnan as a case study, the results show the following: (1) The three indicators reveal the scenic road’s distinctive visual landscape characteristics, marked by high naturalness, coherence, and relative openness. (2) SRVLE is found to vary between the two driving directions and different sections. (3) The three-dimensional evaluation cube intuitively displays the comprehensive characteristics of landscape quality, providing a basis for scenic road planning. This method offers a new approach to resolving the subjective–objective divide in SRVLE and can assist road administrations in enhancing policy planning, construction, and management, thereby promoting the high-quality development of scenic roads.
Numerical Study on the Summer High-Temperature Climate Adaptation of Traditional Dwellings in the Western Plains of Sichuan, China
Authors: Li Rongjia , Li Qiushan , Mikiko Ishikawa , Wumaier Kabilijiang
Published: August 2024

Ongoing global climate change, marked by sustained warming and extreme weather events, poses a severe threat to both the Earth’s ecosystems and human communities. Traditional settlements that underwent natural selection and evolution developed a unique set of features to adapt to and regulate the local climate. A comprehensive exploration of the spatial patterns and mechanisms of the adaptation of these traditional settlements is crucial for investigating low-energy climate adaptation theories and methods as well as enhancing the comfort of future human habitats. This study used numerical simulations and field measurements to investigate the air temperature, relative humidity, wind speed, wind direction, and thermal comfort of traditional settlements in Western Sichuan Plain, China, and uncovered their climate suitability characteristics to determine the impact mechanisms of landscape element configurations (building height, building density, tree coverage, and tree position) and spatial patterns on microclimates within these settlements. The results revealed the structural and layout strategies adopted by traditional settlements to adapt to different climatic conditions, providing valuable insights for future rural protection and planning and enhancing climate resilience through natural means. These findings not only contribute to understanding the climate adaptability of Earth’s ecosystems and traditional settlements but also offer new theories and methods to address the challenges posed by climate change.
Constructing Soils to Mitigate Land Occupation by Urban Expansion and Metabolism to Improve Healthy Cities
Authors: Rodríguez-Espinosa Teresa , Pérez-Gimeno Ana , Almendro-Candel María Belén , Navarro-Pedreño Jose
Published: August 2024

The number of city residents worldwide is increasing at the same that soil consumption around cities, which can be mitigated using technosols. Urban areas need to provide a healthy environment for residents, but this is threatened by climate change. Mitigating the adverse impacts of climate change does not involve one-size-fits-all global solutions; cities face varying economic and social contexts. Cities need to offer ecosystem services in order to operate as healthy urban ecosystems. The urban soils’ environmental services are often overlooked, leading to public administrations having little to no awareness about land management policies and ecosystem services. Technosols, artificial or human altered soils, have the potential to provide the same ecosystem benefits as natural soils and do not require as much time to develop in order to perform their functions. Additionally, technosols have the potential to enhance the circular economy using waste materials. In this sense, policy makers should incorporate urban technosols as a strategy to enhance the health of cities and address climate change. Our perspective on soils in urban areas needs to be altered, as technosols should be included in urban policies, have the potential to serve as a crucial component in providing ecosystem services and acting as a carbon sink and enhance urban well-being.
A Blood Supply Pathophysiological Microcirculatory Mechanism for Long COVID
Authors: Koutsiaris Aristotle G.
Published: August 2024

Background: The term “Long COVID” is commonly used to describe persisting symptoms after acute COVID-19. Until now, proposed mechanisms for the explanation of Long COVID have not related quantitative measurements to basic laws. In this work, a common framework for the Long COVID pathophysiological mechanism is presented, based on the blood supply deprivation and the flow diffusion equation. Methods: Case–control studies with statistically significant differences between cases (post-COVID patients) and controls, from multiple tissues and geographical areas, were gathered and tabulated. Microvascular loss (ML) was quantified by vessel density reduction (VDR), foveal avascular zone enlargement (FAZE), capillary density reduction (CDR), and percentage of perfused vessel reduction (PPVR). Both ML and hemodynamic decrease (HD) were incorporated in the tissue blood supply reduction (SR) estimation. Results: ML data were found from 763 post-COVID patients with an average VDR, FAZE, CDR, and PPVR of 16%, 31%, 14%, and 21%, respectively. The average HD from 72 post-COVID patients was 37%. The estimated SR for multiple tissues with data from 634 post-COVID patients reached a sizeable 47%. This large SR creates conditions of lower mass diffusion rates, hypoxia, and undernutrition, which at a multi-tissue level, for a long time, can explain the wide variety of the Long COVID symptoms. Conclusions: Disruption of peripheral tissue blood supply by the contribution of both ML and HD is proposed here to be the principal cause of the mechanism leading to Long COVID symptoms.
The Hepatoprotective Effects of Camellia sinensis on Cisplatin-Induced Acute Liver Injury
Authors: Yilmaz Adnan , Dizman Fatih , Akyildiz Kerimali , Mataraci Karakas Sibel , Mercantepe Tolga , Uydu Huseyin Avni , Tumkaya Levent , Ozturk Koksal
Published: August 2024

Acute liver injury is an increasing global health problem. It is a widespread side effect of cisplatin treatment in the clinic and can lead to liver failure if not treated promptly. Previous studies have revealed that green tea can protect some organs from treatments. However, the potential of white tea to prevent the negative effects of acute liver injury has not been addressed so far. The purpose of this study was to investigate the reduction in cisplatin-induced liver injury in rats receiving white tea. Female Sprague Dawley rats with similar weight were selected in this study. Twenty-four rats were divided into three groups of eight animals each and ad libitum nutrition was provided. The control and cisplatin groups were given tap water only, while the white tea + cisplatin group received white tea at a 0.5% weight/volume concentration for four weeks. At the end of the fourth week, the white tea + cisplatin group and the cisplatin group received a single dose of cisplatin (7 mg/kg) via the intraperitoneal route. Five days after that procedure, the rats were anesthetized. Liver tissues and blood samples were collected, which were used for biochemical and histopathological analyses. According to biochemical results, liver tissue MDA and GSH, serum ALT, and AST levels significantly increased in the cisplatin group compared to the control group. Compared with the cisplatin group, although MDA, AST, ALT, and GSH levels were lower in the white tea + cisplatin group, only GSH levels were statistically different. The examination of histopathological and immunohistochemical findings revealed apoptotic cells, vascular congestion, and sinusoidal dilatation in the cisplatin group compared to the control group. This adverse event decreased in the white tea + cisplatin group compared to the cisplatin group. In conclusion, white tea exhibits an ameliorating effect on cisplatin-induced liver injury.
Changes in Surface Topography and Light Load Hardness in Thrust Bearings as a Reason of Tribo-Electric Loads
Authors: Graf Simon , Koch Oliver
Published: August 2024

The article focuses on the findings of endurance tests on thrust bearings. In addition to the mechanical load (axial load: 10 ≤ C0/P ≤ 19, lubrication gap: 0.33 µm ≤ h0 ≤ 1.23 µm), these bearings are also exposed to electrical loads (voltage: 20 Vpp ≤ U0 ≤ 60 Vpp, frequency 5 kHz and 20 kHz), such as those generated by modern frequency converters. In a previous study, the focus was on the chemical change in the lubricant and the resulting wear particles. In contrast, this article focuses on the changes occurring in the metallic contact partners. Therefore, the changes in the surface topography are analysed using Abbott–Firestone curves. These findings show that tests with an additional electrical load lead to a significant reduction in roughness peaks. A correlation to acceleration measurements is performed. Moreover, it is shown that the electrical load possibly has an effect on the light load hardness. An increase in the occurring wear could not be detected during the test series. Also, a comparison with mechanical reference tests is made. The article finally provides an overview of different measurement values and their sensitivity to additional electrical loads in roller bearings.
Enhancing of Surface Quality of FDM Moulded Materials through Hybrid Techniques
Authors: Jabłońska Monika , Łastowska Olga
Published: August 2024

With the rapid advancement of 3D-printing technology, additive manufacturing using FDM extrusion has emerged as a prominent method in manufacturing. However, it encounters certain limitations, notably in surface quality and dimensional accuracy. Addressing issues related to stability and surface roughness necessitates the integration of 3D-printing technology with traditional machining, a strategy known as the hybrid technique. This paper presents a study of the surface geometric parameters and microstructure of plastic parts produced by FDM. Sleeve-shaped samples were 3D-printed from polyethylene terephthalate glycol material using variable layer heights of 0.1 mm and 0.2 mm and then subjected to the turning process with PVD-coated DCMT11T304 turning inserts using variable cutting parameters. The cutting depth was constant at 0.82 mm. Surface roughness values were correlated with the cutting tool feed rate and the printing layer height applied. The selected specimen’s microstructure was studied with a Zeiss EVO MA 15 scanning electron microscope. The roundness was measured with a Keyence VR-6200 3D optical profilometer. The research results confirmed that the additional application of turning, combined with a reduction in the feed rate (0.0506 mm/rev) and the height of the printed layer (0.1 mm), reduced the surface roughness of the sleeve (Ra = 1.94 μm) and increased its geometric accuracy.
Effect of Ce Addition on Microstructure, Thermal Conductivity, and Mechanical Properties of As-Cast and As-Extruded Mg–3Sn Alloys
Authors: He Fei-yu , Hu Wen-xin , Liu Li-juan , He Wei , Ma Shao-bo , Zhang Xu-dong , Yang Zheng-hua , Wang Wei
Published: August 2024

In the present research, the impacts of Ce additions at various concentrations (0, 1.0, 3.4, and 4.0 wt.%) on the evolution of the microstructure, mechanical properties, and thermal conductivity of as-cast and as-extruded Mg-3Sn alloys were investigated. The findings demonstrate that adding Ce caused the creation of a new ternary MgSnCe phase in the magnesium matrix. Some new Mg17Ce2 phases are generated in the microstructure when Ce levels reach 4%. The thermal conductivity of the Mg-3Sn alloy is significantly improved due to Ce addition, and the Mg-3Sn-3.4Ce alloy exhibits the highest thermal conductivity, up to 133.8 W/(m·K) at 298 K. After extrusion, both the thermal conductivity and mechanical properties are further improved. The thermal conductivity perpendicular to the extrusion direction of Mg-3Sn-3.4Ce alloy could achieve 136.28 W/(m·K), and the tensile and yield strengths reach 264.3 MPa and 227.2 MPa, with an elongation of 7.9%. Adding Ce decreases the dissolved Sn atoms and breaks the eutectic α-Mg and Mg2Sn network organization, leading to a considerable increase in the thermal conductivity of as-cast Mg-3Sn alloys. Weakening the deformed grain texture contributed to the further enhancement of the thermal conductivity after extrusion.