bluuebunny/crossref_metadata_2025 huggingface dataset:
Peningkatan Daya Saing Kopi Dengan Strategi Kemasan Dan Pemasaran Di Dusun Borojabung
Authors: Bastomi Mohamad , Fadila Inayatul , Nu’man M. Syauqy , Putri Handini Eka , Zahro Azizatuz , Syamsudin M. Nur , Aminudin Khoirul , Mardliyah Mahnunatul , Hakim Taufiiqul
Published: December 2024

Dusun Borojabung memiliki banyak sekali UMKM, salah satunya adalah UMKM kopi. UMKM kopi sendiri dipilih karena dalam proses pembuatan, pengemasan dan pemasarannya masih menggunakan cara yang sederhana. Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan kualitas kemasan. Metode yang digunakan dalam pengabdian ini adalah sosialisasi, penyuluhan, pelatihan, dan pendampingan. dari kegiatan pengabdian ini, mampu meningkatkan daya saing kopi diluar sana, dari pengemasan yang sudah mengikuti perkembangan zaman dan juga pemasaran di online shop, sehingga mampu menarik lebih banyak pembeli dan juga memperluas pemasaran dengan meningkatkan penjualan di marketplace.
Pemberdayaan Ibu PKK dalam Mengelola Limbah Anorganik sebagai Media Sosialisasi Masyarakat Peduli Lingkungan dan Pendidikan di Desa Kudu Jombang
Authors: Susilo Claudya Zahrani , Saraswati Sari , Rodliyah Iesyah
Published: December 2024

Kegiatan ini bertujuan untuk memberi pengetahuan dasar kepada Masyarakat tentang pendidikan lingkungan hidup berupa pemanfaatan limbah anorganik. Pemanfaatan limbah anorganik adalah satu cara yang bisa dilakukan oleh masyarakat untuk menjaga kelestarian lingkungan. Hasil kegiatan ini diharapkan dapat memberikan kontribusi terhadap kelestarian lingkungan sehingga pencemaran lingkungan dapat dikurangi. Pengetahuan dasar yang diberikan kepada ibu PKK dalam kegiatan ini yaitu tentang: (1) Pengertian dan jenis sampah, (2) pengelolaan sampah anorganik menjadi barang yang bernilai tinggi, (3) pengelolaan sampah organik menjadi pupuk tanaman Kegiatan ini bertempat di Desa Bendungan Kecamatan Kudu, Jombangdengan melibatkan ibu PKK yang berjumlah ±30 orang. Metode yang digunakan adalahmetode ceramah, praktik, dan tanya jawab. Berdasarkan hasil evaluasi yang dilakukan menunjukkan bahwa ibu PKK mampu menerapkan teori yang diberikan dan mempraktikkannya. Hal ini dapat dilihat dari tugas-tugas yang diberikan kepada ibu PKK yaitu membuat produk hasil pengolahan limbah anorganik berupa botol plastik menjadi barang yang bernilai tinggi. Untuk kedepannya, harapan dari kegiatan ini adalah agar para peserta bisa mempraktikkan di masyarakat dan mengajak warga lain untuk menjaga kelestarian lingkungan dengan tidak membuang sampah di sungai, serta mampu mengurangi limbah anorganik agar tidak mengakibatkan kerusakan lingkungan.
Penerapan Metode Pomodoro Dan Cornell Notes Guna Meningkatkan Kualitas Belajar Siswa di MI Al-Marhamah
Authors: Bastomi Mohamad , Aisya Adinda Alifia , Nisa’i Chayrun , Suhartatik Diah Putri , Rahmawati Iis , Fauziah Nanik , Hidayah Nikmatul , Amalya Rifky , Salim Vincencia Carolina
Published: December 2024

MI-Almarhamah students tend not to be able to take part in learning activities well. The majority of them are hyperactive students and cannot focus during long activities. This causes the class atmosphere to be not conducive and student learning outcomes are low. This service aims to improve the quality of student learning at MI-Almarhamah. The methods used are socialization, training and direct mentoring in class as well as monitoring and evaluation related to the application of the Pomodoro and Cornell Notes methods. This community service is able to improve the quality of student learning, make the classroom atmosphere more conducive and help teachers apply appropriate methods for students.
Penyusunan Laporan Keuangan dan SPT Tahunan Wajib Pajak UMKM
Authors: Nandiroh Umi , Hidayati Irma , Badria Nuril
Published: December 2024

The low tax compliance of MSMEs is due to tax policies that are too burdensome for them. Tax regulations are burdensome for MSMEs, thus affecting the growth of this sector. Facts on the ground show that MSME actors often feel bothered by the applicable tax provisions. Starting from the complexity of tax administration, which must be passed in carrying out tax obligations. Plus the low competence of SMEs in preparing financial reports, as a basis for calculating taxes, and attachments in reporting Annual Tax Returns (Annual SPT). This is the reason behind the existence of this service activity. The aim is to conduct socialization, training, and assistance to MSME actors in compiling financial reports and reporting Annual SPTs. Where this service is carried out in the MSME community in the Blimbing sub-district, Malang City. This activity lasts for 3 months, from January to March 2022. The stages carried out are in the form of socialization, training in the preparation of MSME financial reports, and SPT Reporting Assistance. This service activity has succeeded in increasing MSME tax compliance. And MSMEs who are participants in this activity feel very helpful, in compiling financial reports, as controls in financial management, and reporting SPT.
Mengenali Kepribadian Dan Kaitannya Dalam Pemilihan Jurusan Kuliah Bagi Siswa SMA
Authors: Mufarrihah Iftitaahul , Andriani Anita , Lazulfa Indana
Published: December 2024

Personality is a characteristic that owned by each individual. There are different type of personality, and this makes each individual are unique. And its relation to the choice of majors is very important. By understanding one’s personality, one can determine what majors that matches one’s interest and talent. So that in the future they can develop their talent and achieve what they want.
Finplan: Strategi Menuju Keluarga Sejahtera
Authors: Pertiwi Dwi Ari , Agustina Rachma , Ardiana Meta
Published: December 2024

The main problem for a family that is always there is usually around finances, financial management is very important in maintaining economic stability in a family, with planned financial management it will have an impact on success for the family and ease in achieving financial goals. The method of implementing the Community Service Program is by giving lectures and training in financial planning in an easy and simple way. This service activity begins with an analysis of the problem situation that occurs in partner institutions or objects of service, then it is continued with a literature review according to the problems that are given solutions through the PKM program.
Pengembangan Banana Park Kelurahan Warugunung Kota Surabaya Sebagai Rintisan Daerah Wisata
Authors: Aji Tony Seno , Rachmawati Lucky , Afifah Choirul Anna Nur
Published: December 2024

Pengelolaan taman Banana Park Kelurahan Warugunung Kota Surabaya masih belum tergerak dengan baik dan manajemen ekowisata yang masih stagnan. Diperlukan peningkatan mutu dan manajemen ekowisata serta partisipasi aktif masyarakat dalam memajukan obyek wisata, sehingga obyek wisata Banana Park mampu menjadi penopang ekonomi bagi Warga Sekitar Banana Park. Kontribusi mendasar kegiatan PKM pada khalayak sasaran: 1. perbaikan sarana prasarana area wisata banana park, 2. memberikan pengetahuan tentang pemanfaatan informasi teknologi promosi, 3. memberikan pengetahuan tentang ketrampilan dalam pembuatan oleh-oleh wisata. Progam PKM berjalan dengan lancar. Dari angket yang sudah disebar, para peserta menyatakan: 1) Materi yang disampaikan jelas, runtut dan mudah dipahami serta ditiru; 2) Bahan-bahan dan alat pembuatan kue sederhana mudah diperoleh dan harganya terjangkau, 3) Peserta ingin mengembangkan produk pisang dan memanfaatkan informasi teknologi promosi lebih lanjut; 4) Pengembangan produk pisang dirasa layak untuk dikonsumsi dan dijual; 5) Peserta bersedia memberitahukan kepada keluarga atau tetangga tentang materi yang disampaikan; 6) Kegiatan PKM menarik dan bermanfaat.
Pelatihan Ice Breaking melalui Kegiatan Workshop di SDN Jombatan 3 Jombang
Authors: Nuruddin Muhammad , Raharja Hawwin Fitra , Siswanto M. Bambang Edi , Permatasari Mega Indah , Pratiwi Emy Yunita Rahma , Asmarani Ratih , Rochmania Desty Dwi
Published: December 2024

Pada sebuah proses kehidupan, seseorang tidak akan lepas dengan yang namanya belajar. Ketika proses pembelajaran terjadi, maka akan ada pengalaman belajar yang didapat. Proses ini akan efektif jika dilakukan dengan yang menyenangkan. Agar pembelajaran lebih menyenangkan, guru dapat menggunakan ice breaking. Hal ini dilakukan guna suasana belajar lebih menyenangkan dan siswa tidak mudah bosan dan tertarik untuk mengikuti kegiatan belajar mengajar. Workshop pelatihan ice breaking ini diadakan oleh tim dosen PGSD Unhasy berkolaborasi dengan tim AMSP kelompok 13 Unhasy yang bertempat di SDN Jombatan 3 Jombang. Kegiatan ini dikemas dalam kegiatan PKM (Pengabdian Kepada Masyarakat), dan yang menjadi sasaran atau peserta adalah para guru di SDN Jombatan 3 Jombang. Proses kegiatan ini dimulai dengan kegiatan pra acara, kegiatan pemaparan materi, kegiatan praktik ice breaking, dan tugas mandiri. Melalui kegiatan workshop pelatihan ini, diharapkan pembelajaran di kelas menjadi lebih hangat dan menyenangkan, sehingga menambah semangat siswa untuk antusias kembali dalam mengikuti kegiatan belajar mengajar di kelas.
Pengenalan Koperasi Syariah Dalam Mensejahterakan Perekonomian Umat
Authors: Miko Jeroh
Published: December 2024

Koperasi syariah merupakan bentuk koperasi yang memiliki prinsip, tujuan, dan kegiatan usahanya berdasarkan syariah Islam, yaitu Al-quran dan As-sunah. Secara umum, koperasi ini merupakan badan usaha koperasi yang menjalankan aktivitas usahanya berdasarkan pada prinsip-prinsip syariah. Tujuan koperasi syariah harus sesuai dengan Maqashid Syariah yang fungsinya untuk melakukan dua hal penting, yaitu tahsil, yakni mengamankan manfaat (manfaah) dan ibqa, yaitu mencegah kerusakan atau cedera (madarrah) seperti yang diarahkan oleh Pemberi Hukum. Tujuan koperasi syariah yaitu mensejahterakan ekonomi anggotanya sesuai norma dan moral Islam, menciptakan persaudaraan dan keadilan sesama anggota, pendistribusian pendapatan dan kekayaan yang merata sesama anggota berdasarkan kontribusinya, kebebasan pribadi dalam kemaslahatan sosial yang didasarkan pada pengertian bahwa manusia diciptakan hanya untuk tunduk pada Allah, meningkatkan kesejahteraan anggota pada khususnya dan masyarakat pada umumnya serta turut membangun tatanan perekonomian yang berkeadilan sesuai dengan prinsip-prinsip Islam.
Pendampingan Pembuatan Sertifikasi Halal bagi UMKM di Desa Sidokaton Jombang
Authors: Mufarrihah Iftitaahul , Lazulfa Indana
Published: December 2024

Perekonomian Indonesia pasca Covid 19 mulai berkembang pesat dengan ditandainya semakin banyaknya UMKM yang ada dimasyarakat.  UMKM banyak menyerap tenaga kerja setelah adanya PHK saat Covid 19 melanda. Salah satu usaha yang dilakukan pemerintah untuk meningkatkan kualitas UMKM adalah dengan melakukan sertifikasi halal. Tujuan adanya sertifikasi halal ini adalah untuk meninkatkan keprcayaan masyarakat terhadap produk UMKM dan agar pelaku UMKM di Indonesia mampu bersaing di tingkat global. Sayangnya pengetahuan masyarakat tentang pentingnya sertifikasi halal ini masih sangat kurang, dan menganggap bahwa prosesnya sulit dan berbelit. Oleh karena itu diadakanlah pendampingan pembuatan sertifikasi halal pada UMKM yang ada di Desa Sidokaton agar para pelaku UMKM di desa tersebut dapat meningkatkan kualitas produk yan dihasilkan dan dapat bersaing dengan UMKM sejenis lainnya. Hasil kegiatan ini adalah semua UMKM yang ada di Desa Sidokaton sudah memiliki sertifikat halal. Kegiatan ini mendapatkan dukungan dan sambutan yang baik dari pemerintah desa maupun masyarakat sebanyak 85% menilai sangat membantu dan sisanya menilai baik dan cukup
Pengarahan Peningkatan Kapabilitas Usaha Mandiri Bolen Pisang melalui Marketing Mix di desa Bibrik Madiun
Authors: K Kusnul Ciptanila Yuni , Sopingi Imam , Haryanti Peni , Hidayati Athi' , Musfiroh Anita , Rifardi Lailatul Maghfiroh Putri , Sobaroh Lutfia
Published: December 2024

Usaha mandiri bolen pisang di desa Bibrik Madiun merupakan bagian integral dari ekonomi lokal yang membutuhkan perhatian dalam meningkatkan kapabilitasnya. Pengarahan ini bertujuan untuk mengeksplorasi penerapan strategi marketing mix sebagai upaya untuk meningkatkan daya saing usaha ini. Metode dalam pengarahan ini menggunakan program bantuan strategi atau metodologi Asset-Based Community Development (ABCD). Hasil pengarahan menunjukkan bahwa tingkat pengetahuan masyarakat mengenai stunting sebelum dilakukanya monitoring/pengarahan hanya 60% yang menjawab benar dari seluruh peserta penyuluhan, dan setelah dilakukan monitoring/pengarahan hasilnya meningkat 20%. Sehingga dapat disimpulkan bahwa kegiatan yang dilakukan mengenai pengetahuan marketing mix masyarakat berhasil meningkat menjadi 80%. Artinya terdapat dampak positif dari pemberian monitoring sekaligus materi pengetahuan marketing mix terhadap ibu kader PKK dan ibu-ibu masyarakat desa Bibrik.
Edukasi Peran Inklusi Keuangan Syariah Dalam Meningkatkan Stabilitas Ekonomi Masyarakat
Authors: Arafah Santi , Miko Jeroh , Ria
Published: December 2024

Tujuan kegiatan pengadian ini ialah untuk mengedukasi peran inklusi keuangan syariah dalam meningkatkan stabilitas ekonomi masyarakat. Metode pelaksanaan meliputi tiga tahap, yaitu observasi, penyampaian materi dan evaluasi pada para remaja Masjid Jami’ Ulayat Percut Sei Tuan selama tiga bulan. Hasil dari pengabdian ini para peserta mulai memahami pengertian inklusi keuangan syariah yaitu ketersediaan akses terhadap produk keuangan syariah yang sesuai termasuk pembiayaan, tabungan, asuransi dan pembayaran, tersedianya akses yang berkualitas termasuk kenyamanan, keterjangkauan, kesesuaian dan dengan memperhatikan perlindungan konsumen serta ketersediaan tersebut juga diberikan kepada semua orang. Inklusi keuangan syariah ini bertujuan untuk mengubah pola pikir pelaku usaha tentang cara melihat uang dan laba, dengan pola pikir tersebut maka akan mengubah pelaku ekonom menjadi responsive. Inklusi keuangan syariah termasuk dalam program literasi keuangan syariah, dikarenakan jika peningkatan inklusi keuangan syariah semakin meningkat maka akan meningkatkan stabilitas ekonomi masyarakat bagi suatu negara
Pocket Book Sebagai Sarana Pengelolaan dan Perencanaan Keuangan
Authors: Ardiana Meta , Pertiwi Dwi Ari , Agustina Rachma
Published: December 2024

Setiap entitas hendaknya mengetahui berapa besar serapan biaya operasional usaha, berapa proyeksi profit yang terealisasi, serta berapa ekuitas yang dikelola untuk usaha. Sehingga dapat dijadikan alat evaluasi kemampuan dan kapasitas usaha sehingga dapat merancang strategi untuk pengembangan usahanya tersebut berdasarkan pencatatan keuangan yang telah dilakukan. Pengelolaan dan perencanaan keuangan bisa dimulai dengan disiplin melakukan pencatatan keuangan, pencatatan keuangan bisa dilakukan pada buku kecil (pocket book) maupun menggunakan aplikasi software dan gadget. Metode yang digunakan untuk memecahkan masalah terkait pengelolaan keuangan yakni melalui sosialisasi, ceramah, tutorial, pendampingan dan diskusi pengelolaan keuangan dengan pocket book di SMK Matsna Karim. Hasil pengabdian ini diketahui peserta mampu menyerap materi yang sampaikan dan antusias untuk menerapkan pencatatan keuangan dengan menggunakan pocket book. Penerapan pocket book ini diharapkan mampu meningkatkan pengelolaan keuangan pribadi maupun unit entitas. Dengan pencatatan  keuangan secara rutin dan disiplin. Unit usaha dapat meningkatkan profit dan keunggulan bersaing.
Penerapan Manajemen Risiko Berbasis Syariah Dalam Mengelola Keuangan Organisasi
Authors: Kurniawan Rahmat , Hutagalung M. Abrar Kasmin , Rambe Riswan , Nasution Rana
Published: December 2024

Penerapan manajemen risiko dalam mengelola keuangan organisasi merupakan hal yang sangat fundamental. Pencapaian tujuan organisasi setiap komponen organisasi harus ditigasi risiko sedini mungkin agar efektif dan efisien. Konsekuensi logisnya, jika organisasi memiliki manajemen risiko yang terukur, maka segala bentuk kerugian organisasi dapat diminimalisir. Metode pengabdian ini dilakukan dengan pelaksanaan seminar bertema “Penerapan Manajemen Risiko Berbasis Syariah dalam Mengelola Keuangan Organisasi”. Kegiatan ini dilaksanakan di Masjid al-Amanah Tanjung Mulia Medan dengan menggunakan aplikasi Ms. Power Point sebagai presentasi materi. Hasilnya para pengurus masjid termotivasi untuk menerapkan manajemen risiko berbasis syariah dalam mengelola keuangan organisasi.
Peran Literasi Keuangan Syariah Dalam Membangun Kesadaran Generasi Milenial Di Era Revolusi Industri 5.0
Authors: Miko Jeroh , Arafah Santi , Hariani Fina
Published: December 2024

Peran literasi keuangan syariah menjadi semakin krusial di era Revolusi Industri 5.0, terutama bagi generasi milenial yang kerap menghadapi tantangan dalam pengelolaan keuangan. Pengabdian ini bertujuan untuk meningkatkan kesadaran dan pemahaman milenial tentang literasi keuangan syariah, guna membantu mereka membuat keputusan finansial yang lebih bijak dan etis. Metode pelaksanaan pengabdian meliputi perencanaan dan pelaksanaan. Perencanaan terdiri dari (1) melakukan studi literatur mengenai peran literasi keuangan syariah bagi generasi milenial dalam mengelola keuangan; (2) menentukan waktu pelaksanaan dan durasi kegiatan pengabdian bersama dengan tim pelaksana; (3) menentukan dan mempersiapkan materi yang akan disampaikan dalam kegiatan pengabdian kepada masyarakat. Sedangkan pelaksanaan kegiatan dilakukan dengan metode pengajaran yang dilakukan di dalam kelas dengan menggunakan slide dan presentasi. Hasilnya para peserta menjadi lebih mengetahui tentang literasi keuangan syariah dan menjadi sadar pentingnya mengetahui konsep literasi keuangan syariah.
Penguatan Ecopreneurship untuk Meningkatkan Minat Berwirausaha Pelajar
Authors: Falah Fajrul , Af’idah Noer , Rochim Roudhoutul Aulia , Musfiroh Anita
Published: December 2024

Pelatihan ecopreneurship kepada siswa Madrasah Aliyah dilakukan sebagai salah satu upaya untuk mendukung pemerintah dalam menciptakan generasi muda yang memiliki jiwa entrepreneur yang tangguh.  Adapun tujuan dari kegiatan pelatihan ini adalah untuk memberikan edukasi tentang wawasan kewirausahaan dan pengalaman kepada siswa dalam menghasilkan produk kewirausahaan berbasis lingkungan hidup. Kegiatan pengabdian ini dilaksanakan dengan melalui tiga tahap yaitu, tahap persiapan, tahap pelaksanaan, serta tahap refleksi dan tahap evaluasi. Kegiatan pelatihan ini dilaksanakan selama dua hari pada tanggal 23-24 April 2024 di MASS Khoiriyah Hasyim Seblak Jombang. Peserta kegiatan pelatihan adalah siswa kelas X dan XI IPA Putri sebanyak 31 orang. Hasil kegiatan ini menunjukkan bahwa sebanyak 83,87% peserta aktif selama mengikuti pelatihan; nilai ketrampilan dari pembuatan produk sebesar 82; dan skor minat berwirausaha sebesar 81,5.
Pelatihan Penerapan ISAK 35 Pada Panti Asuhan Pelita Harapan dan Penuh Pengharapan
Authors: Purba Sahala , Purba Kuras , Siahaan Septony B , Sitohang Santy Aji , Nababan Dame Tiur Magdalena
Published: December 2024

Maksud dari pelatihan ini untuk mengembangkan panti asuhan dalam hal akuntabilitas maupun pelaporan keuangan sesuai dengan ISAK 35. Adapun metode yang digunakan dalam pelatihan ini adalah dengan cara wawancara terlebih dahulu lalu melakukan pelatihan pencatatan akuntansi sampai pelaporan keuangan sesuai dengan regulasi yang berlaku di Indonesia yaitu ISAK 35, dengan adanya pelatihan ini diharapkan  panti asuhan dapat  melakukan pertanggungjawaban atas sumbangan dari donatur. Pelatihan ini dilakukan selama 2 (dua) hari yaitu pada tanggal 8 dan 9 Desember 2023. Adapun hasil dari pelatihan ini adalah para pemilik panti asuhan merasa canggung karena selama ini mereka tidak mengetahui ada standar pelaporan keuangan khususnya untuk panti asuhan, karena mereka mencatat keuangannya hanya bentuk penerimaan kas dan pengeluaran kas saja, itupun dilakukan pada papan pengumuman yang ada dalam panti asuhan tersebut. Adapun yang menjadi implikasi dalam pelatihan ini adalah agar panti asuhan dapat berkelanjutan pada masa yang akan datang.
Governance of Healthcare Policy through the Optimization of Organizational Citizenship Behavior
Authors: Deviyantoro Deviyantoro , Huddin Muhammad Nurhaula , Wajdi Farid , Ramazanu Sheena , Agustin Aras Nurbarich
Published: December 2024

Purpose – This research aimed to identify person-organization (P-O) fit, person-job (P-J) fit, job satisfaction, and Organizational Citizenship Behavior (OCB).
Methodology – A quantitative method was adopted through an online survey applied to 323 respondents who were healthcare employees in Banten Province. The data obtained was later analyzed using Structural Equation Model (SEM) Amos.
Results – The results showed that P-O fit positively correlated with job satisfaction and OCB. Moreover, P-J fit had a positive effect on OCB. Job satisfaction also had a positive effect on OCB. The implication is that managers need to focus on ensuring a connection between organizational values and those held by healthcare professionals to achieve job satisfaction. There is also the need for management to emphasize ensuring the skills and abilities of healthcare professionals are in line with job roles in order to improve job satisfaction.
Originality – The novelty of this research was in the simultaneous examination of both P-O fit and P-J fit in order to provide a more comprehensive understanding of the combined influence on OCB and its dimensions. Furthermore, a novel exploration of the underlying psychological mechanisms was introduced based on social exchange theory to explain how perceptions of fit motivated employees to engage in OCB.
Nostalgia em "Cuphead" (2017)
Authors: Falqueto Lemos Adriana , Móniva Vermes Viviana
Published: December 2024

Este texto traz discussões baseadas no estudo do jogo Cuphead (2017), utilizando uma abordagem qualitativa para coleta de dados do jogo. O estudo tem como ponto de partida a breve revisão bibliográfica acerca dos estudos de jogos e tem como perspectiva a noção de que os jogos são objetos de leitura, assim como postulado por estudos chartierianos. A leitura de Cuphead foi feita a fim de depreender de que forma seu texto sincrético afeta o jogador, provocando a emoção de nostalgia. Ao final da pesquisa empreendida, observou-se que o jogo é uma amálgama de referências imagéticas e sonoras de uma 1930 imaginada, organizada sem um olhar crítico a respeito do preconceito racial presente nas colagens que originaram os desenhos animados da chamada década de ouro da animação estadunidense.
Liquid–liquid phase separation in hepatocellular carcinoma
Authors: Xu Jianguo , Liu Wangwang , Yao Yihan , Knowles Tuomas P. J. , Zhang Zhi-Gang , Zhang Yan-Li
Published: December 2024

Liquid-liquid phase separation (LLPS) drives the formation of membraneless intracellular compartments within both cytoplasm and nucleus. These compartments can form distinct physicochemical environments, and in particular display different concentrations of proteins, RNA, and macromolecules compared to the surrounding cytosol. Recent studies have highlighted the significant role of aberrant LLPS in cancer development and progression, impacting many core processes such as oncogenic signalling pathways, transcriptional dysregulation, and genome instability. In hepatocellular carcinoma (HCC), aberrant formation of biomolecular condensates has been observed in a number of preclinical models, highlighting their significance as an emerging factor in understanding cancer biology and its molecular underpinnings. In this review, we summarize emerging evidence and recent advances in understanding the role of LLPS in HCC, with a particular focus on the regulation and dysregulation of cytoplasmic and nuclear condensates in cancer cells. We finally discuss how an emerging understanding of phase separation processes in HCC opens up new potential treatment avenues.
The risk factors and prediction model for postoperative pneumonia after craniotomy
Authors: Xiang Bingbing , Yi Mingliang , Li Chunyan , Yin Hong , Wang Shun , Liu Yiran
Published: December 2024

Background

Craniotomy is highly susceptible to postoperative pneumonia, which significantly impacts the outcomes of patients undergoing such procedures. Our study aims to examine the risk factors associated with postoperative pneumonia and establish a predictive model with a nomogram to assess this risk.

Methods

We conducted a matched 1:1 case-control study involving 831 adult patients undergoing craniotomy at our hospital. Cases consisted of patients who developed postoperative pneumonia within 30 days after surgery, as defined by consensus criteria. Controls were randomly selected from a pool of eligible patients.

Results

The overall incidence rate of postoperative pneumonia is 12.39% in a total of 831 surgeries, which associated with unfavorable outcomes. Gram-negative bacteria were found to be the most common causative agents and approximately 27.94% of cases attributed to multi-drug resistant strains. The logistic regression analysis revealed five independent risk factors, as follows: smoking history, surgical duration, postoperative albumin, unplanned re-operation, and deep vein catheterization. A risk prediction model was derived and a nomogram was constructed. The Hosmer-Lemeshow test yielded X2 = 3.871 (P=0.869), and the receiver operator characteristic curve analysis demonstrated an area under the curve of 0.898 (P<0.05), with a sensitivity of 79.6% and a specificity of 85.4%, indicating excellent model fit and predictive performance. In addition, the C-index of the nomogram model was 0.898(95%CI, 0.853~0.941). The calibration curves of the nomogram model showed p-values of 0.797 and the Brier scores were 0.127. The analysis of the clinical decision curve showed that the nomograph model had high clinical application value.

Conclusions

Postoperative pneumonia patients after craniotomy exhibits distinct pathogen distribution and is strongly associated with unfavorable outcomes. The risk prediction model developed in this study demonstrates a good fitting degree and predictive performance. The constructed nomogram model is objective, specific, and easily applicable in clinical practice.
Prevalence and predictors of poor heart failure treatment outcomes in Ethiopia: a systematic review and meta-analysis
Authors: Bekele Firomsa , Tafese Lalise , Fekadu Ginenus , Dube Geleta Nenko , Dugassa Dinka , Samuel Dagim
Published: December 2024

Background

Heart failure (HF) is a leading cause of morbidity and mortality worldwide. Various factors can exacerbate disease progression in patients with HF and negatively impact treatment outcomes. This study aims to evaluate the pooled prevalence and contributing factors associated with poor heart failure treatment outcomes in Ethiopia.

Methods

A systematic review and meta-analysis were conducted using five databases: Google Scholar, ScienceDirect, Hinari, PubMed, and Scopus. In total, 12 studies met the eligibility criteria for inclusion in this analysis. The review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines. Data extraction was performed using a Microsoft Excel spreadsheet, and statistical analysis was conducted with STATA 14. The Joanna Briggs Institute Meta-analysis of Statistics Assessment and Review Instrument was utilized for quality assessment. Heterogeneity among the studies was evaluated using the I2 statistic and the Cochrane Q test. Publication bias was assessed using Begg's test, Egger's weighted regression, and funnel plots.

Results

The pooled prevalence of poor HF treatment outcomes was found to be 16.67% [95% confidence interval (CI): 10.67–22.67]. No significant heterogeneity was observed across the included studies (I2 = 0.0%, p = 0.962). Significant predictors of a poor treatment outcome were smoking cigarettes [adjusted odds ratio (AOR) = 10.74; 95% CI: 3.24–35.63] and medication-related problems (AOR = 3.99; 95% CI: 1.90–8.37).

Conclusion

The prevalence of poor HF treatment outcomes in Ethiopia was found to be high. Smoking cigarettes and medication-related problems are significant predictors of these adverse outcomes. Comprehensive health education and improved clinical pharmacy services are essential for addressing these issues.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023437397, PROSPERO (CRD42023437397).
VA-ECOM assisted percutaneous mechanical thrombectomy treatment high-risk pulmonary embolism
Authors: Zhou Zhenhang , Zhong Yaoyang , Hu Jianbo , Wu Zhonghua , Zou Liping , Deng Zhihe , Bi Guoshan , Shen Xin , Dai Xianpeng , Huang Zhijia , Xiong Guozuo , Xu Yiming , Deng Liming
Published: December 2024

Background

Percutaneous mechanical thrombectomy (PMT) is increasingly used in the treatment of intermediate and high-risk acute pulmonary embolism (PE), and the treatment of high-risk PE with the aid of veno-arterial extracorporeal membrane oxygenation (VA-ECMO) has also been reported. However, there are few reports of VA-ECOM-assisted PMT in the treatment of high-risk PE. The purpose of this study is to summarize the data of 11 patients with high-risk PE treated with VA-ECMO assisted PMT, and propose feasible treatment methods for such patients.

Methods

This multicenter retrospective study included patients with acute high-risk PE who were treated with VA-ECMO-assisted PMT from January 2021 to June 2024. The analysis focused on the right/left ventricle ratio, biomarkers, and pulmonary artery pressure before and after the VA-ECMO-assisted PMT treatment.

Results

All 11 high-risk PE patients suffered cardiac arrest before treatment, computered tomograhy pulmonary angiography (CTPA) confirmed the diagnosis of PE, and all patients received VA-ECMO-assisted PMT therapy. The median age of the 11 patients was 54 years (range 18–72), the median duration of ECMO was 4.48 days (range 1.04–18.02), and the mean hospitalization time was 21 days (range 14–112). All patients received percutaneous thrombectomy, achieving a 100% technical success rate. The mortality rate was 27.3% during the 90-day follow-up. The 12-month mortality rate was 36.4%.

Conclusion

VA-ECMO-assisted PMT technology can rapidly improve pulmonary hemodynamics while maintaining stable blood flow, thereby reducing in-hospital mortality in high-risk patients with pulmonary embolism complicated by cardiac arrest.
Determination of the fracture closure pressure in fractural-cavity carbonate reservoirs using a failure criterion based on asperity behavior
Authors: Tian Yuanyuan , Chen Qing , Wu Jvlin , Li Kai , Yan Changhui , He Yi
Published: December 2024

As fluid flow paths in fractural-cavity carbonate reservoirs, fractures have a significant impact on the production performance of carbonate reservoirs. In particular, well production depends on the apertures of the fractures, which vary with the effective stress acting on the fractures. Thus, predicting the fracture closure pressure is crucial for carbonate reservoir development. In our research, fracture closure pressures are derived using the Zienkiewicz–Pande failure criterion, which defines the pressure at which most asperities come into contact. The results reveal that fracture closure is influenced by the geo-stress field, rock mechanics, and spatial location of the fracture. Ultimately, the fracture closure pressure of typical wells located in different tectonic zones in the Shunbei Oilfield is calculated, and the results indicate that the fracture closure pressure in the Shunbei Oilfield is significantly affected by the dip of fractures and the angle between the fracture strike and maximum principal stress. To demonstrate the accuracy of the estimated fracture closure pressure, production performance corresponding to fracture closure was evaluated. It reveals that the flowing bottom pressure decreases rapidly and the recoverable oil reserves reduce when the pressure approaches the fracture closure pressure. This observation verifies that the fracture closure pressure determined using our formula is a feasible predictor of the production performance of fractural-cavity carbonate reservoirs.
New insights into the management of homozygous familial hypercholesterolemia patients treated with lomitapide: a single-center experience
Authors: Iannuzzo Gabriella , Calcaterra Ilenia Lorenza , Gentile Marco , Stanzione Claudia , de Ruberto Francesca , di Taranto Maria Donata , Cardiero Giovanna , Fortunato Giuliana , Minno Matteo Di
Published: December 2024

Familial hypercholesterolemia (FH) is a genetic disease, usually with onset during childhood, characterized by elevated blood LDL cholesterol levels and potentially associated with severe cardiovascular complications. Concerning mutated genes in FH, such as LDLR, a small subset of FH patients presents a homozygous genotype, resulting in homozygous FH (HoFH) disease with a generally aggressive phenotype. Besides statins, ezetimibe and PCSK9 inhibitors, lomitapide (an anti-ApoB therapy) was also approved in 2012–2013 as an adjunctive treatment for HoFH. Despite its clinical efficacy, lomitapide administration should be done with caution because of the possible occurrence of side effects, such as hepatosteatosis, increased blood transaminase levels and gastrointestinal symptoms, as well as the possible deleterious interactions with other drugs. In this context, we decided to report the main available evidence on the management and monitoring of HoFH patients treated with lomitapide and to accompany this literature review with a description of our clinical experience with a subset of six HoFH patients. In conclusion, this paper aims to address an important topic for HoFH-related clinical practice that, to our knowledge, is not yet formally regulated by proper national and/or international guidelines.
Has digital development achieved a synergistic effect of reducing energy intensity and improving carbon emission performance? evidence from China
Authors: Wang Saige , Zhong Honglin , Yang Gang , Zhou Anhua , Liu Jing
Published: December 2024

The rapid growth of the Chinese economy has significantly escalated energy consumption and carbon emissions. The imperative to achieve synergies in energy conservation and carbon reduction has never been more pressing. Digital development presents promising avenues for addressing these challenges, making it crucial to investigate its impact on energy intensity (EI) and carbon emission performance (CEP). This study integrates the super efficiency epsilon-based measure (SE-EBM), mediation effect, and threshold effect models to assess the influence of digital development on EI and CEP using data from 267 cities across China from 2011 to 2019. Our findings demonstrate a notable 23.1% reduction in EI and an 18.5% improvement in CEP attributable to digital development. Moreover, our analysis underscores the pivotal role of technological innovation as a transformative conduit. Importantly, we identify significant threshold effects linked to economic development stages. This study not only enriches our understanding of pathways to energy conservation and carbon reduction but also provides compelling evidence supporting policies aimed at fostering and accelerating digital development initiatives.
Measurement and optimization of sustainable urban form elements in New Urban districts using multi-source data
Authors: Zhang Yanfeng , Wang Xiaohui , Wang Longsheng , Li Guangyu , Zhang Yige , Ye Yu , Jiang Hongqiang , Zhong Shaoyun , Song Junxi
Published: December 2024

Introduction

At the crucial stage of China’s transition from extensive expansion driven by external factors to intensive, connotation-focused development, scientifically optimizing the spatial form of new urban districts, promoting dynamic and adaptive spaces, and enhancing the quality and diversity of urban environments are issues of substantial importance.

Methods

Based on urban morphology, this study proposes that high street accessibility, appropriate building density and typology, and a sufficient degree of functional mixing are the foundations of spatial morphology for promoting sustainable urban development. Using spatial syntax, a spatial matrix, and mixed-function indices, this study measures urban spatial morphology elements and explores differences and rhythms in sustainable spatial morphology in new urban districts with varying development functions and cycles.

Results

The results of the study show that: 1) the overall synergy of the accessibility spatial network in the new urban districts of Yinzhou and Xianlin is high. The building typology was dominated by mid-rise slab and enclosed types, low-rise enclosed types, and high-rise slab types, while the multistory high-coverage forms were interspersed with intervals. 2) Development in new urban districts primarily adopted a large land plot development mode with a single function. The degree of multifunctional mixing was observed to be relatively low. The dual-functional mixing degree in the Yinzhou New Urban District is higher than that in the New Xianlin urban district. 3) A significant level of overlap was observed among functional mixing, accessibility, building density, and typology. Of the functional mixing units, 42.41% of the dual-function and 78.57% of multi-function types were clustered in high-value areas of urban vitality. Additionally, 50.25% of dual-function and 85.71% of multi-function units were aggregated in high-accessibility areas.

Discussion

The mixed-use of sites contributes to the creation of urban vitality and sustainability, and the effect of the correlation between mixed-functionality and accessibility is more pronounced when mixed units involve utility land. The research results provide a reference for evaluating the current sustainable challenges of spatial patterns and offer specific tools for optimizing new urban districts.
Tradeoffs between thinning treatments and rotation periods of planted Larix olgensis forests: a perspective from carbon balance
Authors: Lin Xueying , Lu Wei , Dong Lingbo
Published: December 2024

Introudction

The current CO2 levels are higher than ever in the past two million years. Forests, as one of the climate change mitigation solutions, are becoming increasingly technically feasible and cost-effective. However, limited research comprehensively considers thinning in the context of optimizing the rotation period for carbon sequestration.

Methods

This study utilizes stand-level growth models and diameter distribution models to simulate the carbon balance dynamics of Larch (Larix olgensis) plantations under various thinning scenarios. The effects of different initial planting densities (N0∈{2,500, 3,333, 4,444} tree ha−1) and site class index (SCI∈[14–20] m) on the optimal forest management measures are also quantified.

Results

The results reveal that the overall trend of carbon balance gradually increases and then decreases over time under the baseline scenario (3,333 tree ha−1 of N0, 16 m of SCI, 5% of discount rate, 100 CNY ton−1 C of carbon price); the carbon balances of all thinning forests were less than that of the unthinned forest before until 56th year. The optimal rotation period and net present value (NPV) increase with increasing thinning frequency and intensity. The sensitivity of NPV to thinning frequency increases with higher thinning intensities, SCI, and carbon prices.

Discussion

This study further expands the scope of forest management strategies, providing optimal forest management plans for all 21 combinations of different SCIs and N0. The optimal forest management strategy in the baseline scenario is 3 thinnings, with the first thinning at 20% intensity in the 15th year, the second thinning at 30% intensity in the 18th year, and the third thinning at 30% intensity at 21 years, with a rotation period of 26 years, resulting in an NPV of 37,180 CNY ha−1.
Disparities in adult women's access to contraception during COVID-19: a multi-country cross-sectional survey
Authors: Cavagnis Sara , Ryan Rebecca , Mussa Aamirah , Hargreaves James R. , Tucker Joseph D. , Morroni Chelsea
Published: December 2024

During the COVID-19 pandemic, family planning services over the world have been disrupted. There are still uncertainties about the impact on access to contraception, particularly among marginalised populations. This study aimed to assess the effect of COVID-19 on women's access to contraception, focusing on those experiencing loss of income and self-isolation. The International Sexual Health and Reproductive Health (I-SHARE) survey collected data from 5,216 women in 30 countries. Multivariable logistic regression was conducted to assess the association between loss of income during the pandemic, self-isolation and reduced access to contraception. Women experiencing loss of income and those who had self-isolated had reduced access to contraception (respectively aOR 2.3 and 1.7, for both p < 0.001). Most women reported inaccessibility of health centres, fear of COVID-19, and stockouts as reasons for reduced access. This study highlights how socio-demographic differences may have impacted access to contraception during the pandemic. People experiencing income loss and self-isolation might have faced increased barriers to family planning during the pandemic. Contraception should be prioritised in times of crisis: when planning services, financial support, telehealth and other measures should be implemented in order to increase access and reduce inequalities.
An effective strategy with tofacitinib for the management of focal myositis with rheumatoid arthritis: a case report
Authors: Pan Jing , Zheng Haihong , Xu Juan , Pan Shaobiao
Published: December 2024

Focal myositis is a rare, localized, benign, self-limiting, and non-suppurative inflammatory lesion of the skeletal muscle that may occasionally occur as a complication of rheumatic diseases. This case report discusses a 58-year-old patient with rheumatoid arthritis, who was diagnosed with focal myositis during standard immunosuppressive therapy. The patient was treated with tofacitinib; to our knowledge, this is the first reported case of focal myositis managed with this medication. Clinicians should maintain a suspicion for focal myositis in patients presenting with localized muscle swelling and pain, especially those with underlying rheumatic conditions. In cases where corticosteroid tapering proves difficult, tofacitinib may be considered as an alternative therapeutic strategy.
Optimized BCMA/CS1 bispecific TRuC-T cells secreting IL-7 and CCL21 robustly control multiple myeloma
Authors: Li Min , Zheng Rong , Liu Zairu , Zhang Peiyuan , Zhu Tingwei , Xin Xueyi , Zhao Hongli , Chen Wenyi , Zheng Binjiao , Zhao Ai , Gao Jimin
Published: December 2024

Introduction

Challenges remain in reducing antigen escape and tumor recurrence while CAR-T cell therapy has substantially improved outcomes in the treatment of multiple myeloma. T cell receptor fusion construct (TRuC)-T cells, which utilize intact T cell receptor (TCR)-CD3 complex to eliminate tumor cells in a non-major histocompatibility complex (MHC)-restricted manner, represent a promising strategy. Moreover, interleukin-7 (IL-7) is known to enhance the proliferation and survival of T cells. C-C motif chemokine ligand 21 (CCL21) is a ligand for chemokine C-C motif receptor 7 (CCR7) and exhibits strong chemotaxis against naïve T cells and antigen-presenting cells such as dendritic cells.

Methods

The bispecific TRuC-T cells simultaneously targeting B cell maturation antigen (BCMA) and CD2 subset 1 (CS1) were constructed by pairing two of five subunits (i.e., TCRαC, TCRβC, CD3γ, CD3δ, and CD3ϵ) in the TCR/CD3 complex and were named C-AC-B-3E, C-BC-B-3E, C-3G-B-3E, C-3D-B-3E, C-3E-B-3E, B-3E-C-3E, B-3G-C-3E, and B-3D-C-3E. Additionally, the BCMA/CS1 bispecific TRuC-T cells secreting IL-7 and CCL21, named BC-7×21 TRuC-T cells, were generated. All of the bispecific TRuC-T cells were characterized and tested in vitro and in vivo.

Results

Following the optimization of various pairs of two subunits of TCR/CD3 complex, B-3G-C-3E TRuC-T cells, characterized by incorporating CD3γ and CD3ε, exhibited the strongest myeloma-specific cytotoxicity. Furthermore, the bispecific BC-7×21 TRuC-T cells had stronger proliferation, chemotaxis, and cytotoxicity in vitro. Accordingly, the bispecific BC-7×21 TRuC-T cells showed better persistence in vivo so as to effectively suppress tumor growth in the NCG mouse xenograft model of MM.1S multiple myeloma.

Discussion

This study demonstrated that BC-7×21 TRuC-T cells, engineered through the optimization of the two subunits of TCR/CD3 complex and a co-expression cytokine strategy, may offer a novel and effective therapy for relapsed/refractory multiple myeloma.
CD28 shapes T cell receptor signaling by regulating Lck dynamics and ZAP70 activation
Authors: Raychaudhuri Kumarkrishna , Rangu Rohita , Ma Alison , Alvinez Neriah , Tran Andy D. , Pallikkuth Sandeep , McIntire Katherine M. , Garvey Joseph A. , Yi Jason , Samelson Lawrence E.
Published: December 2024

Introduction

T cell activation requires T cell receptor (TCR) engagement by its specific ligand. This interaction initiates a series of proximal events including tyrosine phosphorylation of the CD3 and TCRζ chains, recruitment, and activation of the protein tyrosine kinases Lck and ZAP70, followed by recruitment of adapter and signaling proteins. CD28 co-stimulation is also required to generate a functional immune response. Currently we lack a full understanding of the molecular mechanism of CD28 activation.

Methods

We employed TIRF microscopy to establish detailed spatial and kinetic relationships among these molecules in live Jurkat and murine primary T cells. We used anti-TCR (CD3) antibodies to trigger formation of TCR microclusters (MC), which are submicron-sized basic signaling units formed during T cell activation. Using this model, we aimed to delineate how the CD28 co-stimulatory signal alters the kinetics and molecular stoichiometry of TCR proximal signaling events, and how these effects could affect the immune response.

Results

Our results show that CD28 co-stimulation specifically accelerated recruitment of ZAP70 to the TCRζ chain in MCs and increased ZAP70 activation. CD28-mediated acceleration of ZAP70 recruitment was driven by enhanced Lck recruitment to the MCs. A greater spatial separation between active and inactive species of Lck was also observed in the MCs as a consequence of CD28 co-stimulation.

Conclusion

These results suggest that CD28 co- stimulation may lower the TCR activation threshold by enhancing the activated form of Lck in the TCR MCs.