bluuebunny/crossref_metadata_2025 huggingface dataset:
Television by the numbers: The challenges of audience measurement in the age of Big Data
Authors: Kelly JP
Published: July 2017

This article examines recent innovations in how television audiences are measured, paying particular attention to the industry's growing efforts to utilize the large bodies of data generated through social media platforms – a paradigm of research known as Big Data. Although Big Data is considered by many in the television industry as a more veracious model of audience research, this essay uses Boyd and Crawford's (2011) `Six Provocations of Big Data' to problematize and interrogate this prevailing industrial consensus. In doing so, this article explores both the affordances and the limitations of this emerging research paradigm – the latter having largely been ignored by those in the industry – and considers the consequences of these developments for the production culture of television more broadly. Although the full impact of the television industry's adoption of Big Data remains unclear, this article traces some preliminary connections between the introduction of these new measurement practices and the production culture of contemporary television. First, I demonstrate how the design of Big Data privileges real-time analysis, which, in turn, encourages increased investment in ‘live’ and/or ‘event’ television. Second, I argue that despite its potential to produce real-time insights, the scale of Big Data actually limits its utility in the context of the creative industries. Third, building on this discussion of the debatable value and applicability of Big Data, I describe how the introduction of social media metrics is further contributing to a ‘data divide’ in which access to these new information data sets is highly uneven, generally favouring institutions over individuals. Taken together, these three different but overlapping developments provide evidence that the introduction of Big Data is already having a notable effect on the television industry in a number of interesting and unexpected ways.
Hacking events: Project development practices and technology use at hackathons
Authors: Richterich Annika
Published: July 2017

Hackathons are techno-creative events during which participants get together in a physical location. They may be hosted by civic communities, corporations or public institutions. Working individually or in teams, usually for several days, participants develop projects such as hardware or software prototypes. Based on a digital ethnography of two events in the Netherlands and Denmark, this article investigates project development practices at hackathons. In particular, it analyses how participants organized their project work and which technologies were used in support of their creative endeavours. Hackathons are increasingly competitive rather than collaborative events, involving time pressure, inducements such as prizes, and requiring efficient skills utilization. I argue that this facilitates the following tendencies: Firstly, strategic effort is put into final presentations. Projects need to be convincingly presented, and persuasively pitching an idea becomes crucial. Secondly, there is only limited time for personal learning, since participants’ existing skills need to be efficiently applied if a team wants to stay competitive. This encourages division of labour within groups: a tendency which seems especially problematic given that IT skills biases are often expressed in terms of gender. Thirdly, participants are more inclined to use technologies that are proprietary but appear ‘open enough’. In light of this observation and by drawing on the concept of technology as resource and opportunity, I discuss the techno-political implications of utilized technologies. With this analysis, I aim at contributing to the critical debate on hackathons as productive but likewise ideologically significant fields of ‘hacking cultures’.
Pics or it didn’t happen: The artwork formerly known as heritage in Google Street Art
Authors: Isaac Joseph
Published: July 2017

In 2014, Google announced a new initiative for its Cultural Institute. Called the Street Art Project, the online database was seen by many (Google included) as a continuation of the Google Art Project, in that it sought to make street art from around the world more easily accessible to interested audiences. The company acknowledged that such art was temporary, and that, by virtue of the artwork’s eventual disappearance, it would eventually become an important record of preservation for the practice. However, in its focus on the physical artwork, the project unwittingly highlights a problematic trend in street art’s protection as intangible heritage: one where its safeguard often becomes overpowered by the tangibility of its material elements. Exploring Google Street Art through the lens of Melbourne, a city that has long negotiated with street art and graffiti as forms of cultural heritage, this article outlines the consequences of such tangibility for the intangible artwork. It argues that any protection of intangible heritage requires a tacit acknowledgement of the subjectivity which surrounds its experience: those community reactions and responses whose wide variability help define that heritage as something intangible.
From Flying Doctor to Virtual Doctor: An Economic Perspective on Australia's Telemedicine Experience
Authors: van Gool K , Haas M R , Viney R
Published: July 2017

Current funding mechanisms can impede the efficient use and integration of telemedicine services. Telemedicine has developed in Australia against a background of complex funding arrangements and interwoven health-care responsibilities. These impediments are not unique to telemedicine but are accentuated by its ability to cover different locations, clinical areas and purposes. There is also a link between economic evaluation and funding mechanisms for telemedicine. While economic evaluations provide important information for the efficient allocation of resources, the funding environment in which telemedicine is established is also crucial in ensuring that services are efficient. Given these complexities, should telemedicine be funded? We conclude that this will depend on: the objectives and priorities of the health system; the efficiency of telemedicine relative to that of other forms of health-care delivery; and the funding environment. In terms of resource allocation processes, the optimum scenario is likely to be where the decision to invest in telemedicine services is made taking local needs into account, but where considerations such as market structure and network compatibility are examined on a broader scale and balanced against the principles of efficiency and equity.
Health Professionals' Attitudes to Videoconferencing in Paediatric Health-Care
Authors: Cohn Richard J , Goodenough Belinda
Published: July 2017

This study surveyed staff involved in paediatric health-care in the state of New South Wales. Questionnaires were sent to: 139 medical professionals in paediatric public health; 157 medical professionals in private practice; 179 nurses; and 125 allied health staff. There were 188 completed surveys (31%). The results showed higher endorsement of videoconferencing for educational or psychosocial applications than for patient management or treatment planning. Medical professionals (especially those in private practice) tended to give the lowest ratings for the potential usefulness of telemedicine. Apart from ratings for various effects of time and distance in the work setting, rural and non-rural professionals generally showed no significant differences, especially in attitudes to and understanding of telemedicine. Hierarchical regression analysis showed that ratings for the future use of videoconferencing (if it were available), in particular ratings for future use of telecommunications technology (e.g. email, telephone conferencing), were determined by factors largely independent of access to telemedicine. The data assist in interpreting the under-use of videoconferencing in health-care.
A Cost-Minimization Analysis of Orthopaedic Consultations Using Videoconferencing in Comparison with Conventional Consulting
Authors: Ohinmaa Arto , Vuolio Saija , Haukipuro Kari , Winblad Ilkka
Published: July 2017

We compared the costs of conventional outpatient visits to the surgical department of the University Hospital of Oulu with those of videoconferencing between the primary care centre in Pyhäjärvi and the University Hospital (separated by 160 km). The cost data were obtained from a randomized controlled trial that included 145 first-admission and follow-up orthopaedic patients. In the telemedicine group the annual fixed costs were €6074 in the hospital and €3910 in the primary care centre. The additional variable costs were €2 in the hospital and €19 in primary care. At a workload of 100 patients, the total cost, including travel and indirect costs, was €87.8 per patient in the telemedicine group and €114.0 per patient in the conventional group (i.e. a total cost saving from the use of teleconsultation of €2620). A cost-minimization analysis showed that telemedicine was less costly for society than conventional care at a workload of more than 80 patients per year. If the distance to specialist care were reduced from 160 km to 80 km, the break-even point increased to about 200 patients per year. Wider utilization of the videoconferencing equipment for other purposes, or the use of less expensive videoconferencing equipment, would make services cost saving even at relatively short distances. The study showed that orthopaedic outpatient telecare can be cost minimizing.
Comparison of Asynchronous and Realtime Teleconsulting for Orthopaedic Second Opinions
Authors: Baruffaldi F , Gualdrini G , Toni A
Published: July 2017

We studied a teleconsulting service for second opinions in orthopaedics. Three units of the national insurance organization for accidents at work were connected to a large orthopaedic hospital in Bologna. During a 20-month study, 65 consultations were provided: 51 (78%) by asynchronous (store-and-forward) consulting and 14 (22%) by realtime videoconferencing. All the consultations made use of radiology images (radiographs, computerized tomography scans, magnetic resonance imaging scans and ultrasound scans). Video-messages and still images were commonly used to support the asynchronous consultations. More data were transmitted on average for an asynchronous teleconsultation (8 MByte) than in a videoconference (5 MByte). The average time spent by orthopaedic specialists was slightly longer in videoconferences (21 min, SD 8) than in asynchronous teleconsultations (19 min, SD 8). The clinicians' confidence in their diagnosis was generally good but was lower in asynchronous consultations. The main problem affecting the telemedicine service was the lack or the low quality of the information received from the referring sites. The clinical complexity of the case and the organizational requirements were declared to be the main factors affecting the choice of consulting procedure. The study showed that the asynchronous method was preferred in the majority of cases and could be easily integrated into clinical practice, although there were some concerns about the diagnostic quality of the information transmitted.
Carnitines Increase Plasma Levels of Adenosine and ATP in Humans
Authors: Capecchi PL , Pasini F Laghi , Quartarolo E , Perri T Di
Published: July 2017

In order to help to clarify the mode of action of carnitine derivatives, plasma levels of adenosine, ATP and inosine were evaluated following the infusion of 0.75, 0.50 and 0.25 mg/kg/min propionyl-L-carnitine (PLC) for 30 min in patients affected with peripheral arterial disease. Moreover, the effects of 0.75 mg/kg/min acetyl-L-carnitine (ALC) and L-carnitine (LC) were studied in the same conditions. Finally, the activity of 7.5 mg/kg/min PLC administered for 3 min was also evaluated.

PLC and ALC produced a significant increase in plasma levels of adenosine and ATP, whereas LC induced less relevant changes. The administration of the compounds did not affect the adenosine/inosine ratio. Peak plasma levels of adenosine preceded in any case those of ATP.

The possibility can be suggested that the pharmacological activity of PLC, ALC, and LC may be mediated, at least in part, by an interference with the endogenous purine system. Since these effects may be related to physiological mechanisms of tissue protection, new pharmacological perspectives for the compounds may arise.
Fibrinogen and Cardiovascular Risk
Authors: Ernst E , Koenig W
Published: July 2017

The hypothesis that fibrinogen is closely related to cardiovascular risk has been strengthened through the results of various lines of research, which this review will aim to unravel.

Several prospective epidemiological studies convincingly show elevated fibrinogen to represent a major, independent cardiovascular risk factor. Cross-sectional studies strongly associate fibrinogen and conventional cardiovascular risk factors. Clinical cohort studies demonstrate that increased fibrinogen is also a risk factor for the sequelae of cardiovascular disease.

Our knowledge about the determinants of the variable plasma level of fibrinogen in health and disease is incomplete. Understanding of the mechanisms that might be involved in the atherothrombogenic action of fibrinogen is also fragmentary. Fibrinogen strongly affects blood coagulation, blood rheology and platelet aggregation. In addition, it has direct effects on the vascular wall and is a prominent acute phase reactant. All of these phenomena might constitute pathophysiological mechanisms involved in the association between fibrinogen and cardiovascular events. Their relative importance is unclear at present.

Even though many crucial questions await conclusive answers, little doubt exists that fibrinogen represents a major, independent risk factor.
Intercultural citizenship in the (foreign) language classroom
Authors: Porto Melina , Houghton Stephanie Ann , Byram Michael
Published: July 2017

The purpose of this special issue is to bring the theory of intercultural citizenship education to readers’ attention and to offer teachers and researchers working with this or similar concepts the opportunity to make their work known in a context of a coherent presentation of theory and practice. In this introduction, we will explain the rationale and the concepts involved in intercultural citizenship education and present the articles in this special issue which arose from our call for papers.
Epidemiology, neurological and functional outcome of concomitant traumatic brain and spinal cord injury: An Oswestry experience
Authors: Ramamurthy Poornashree , Kumar Naveen , Osman Aheed
Published: July 2017

Background

Concomitant traumatic brain injury with spinal cord injury is likely to worsen prognosis and increase hospital length of stay. This study assessed the duration of in-patient rehabilitation and outcome in patients with both traumatic brain injury and spinal cord injury.

Methods

Retrospective study of all patients with concomitant traumatic brain injury and spinal cord injury over a 3-year period who had 5 years of subsequent follow-up at the Midlands Centre for Spinal Injuries.

Results

Twenty-seven patients had concomitant injuries of which five had severe traumatic brain injury, nine had moderate traumatic brain injury and the remaining thirteen had mild traumatic brain injury with spinal cord injury of grades A–D; commonest mechanisms of injury were motor vehicle collision (55%) and falls (37%). Thirteen (48%) had tetraplegia and 14 (52%) had paraplegia. Mean functional independence measure score at admission was 52.1 and 103.4 at 5 years. Patients with mild traumatic brain injury gained a mean functional independence measure score of 67.1; the moderate and severe traumatic brain injury patients gained mean functional independence measure score of 60.1 and 69.2, respectively. The mean length of stay was 138.3, 139.4 and 153.4 days for mild, moderate and severe traumatic brain injury, respectively.

Conclusion

Hospital length of stay and patient’s functioning at 5 years were not affected by traumatic brain injury severity in this subgroup; however, functional independence measure on its own may not be very sensitive to cognitive deficits.
Through a different gate: An automated content analysis of how online news and print news differ
Authors: Burggraaff Christiaan , Trilling Damian
Published: July 2017

We investigate how news values differ between online and print news articles. We hypothesize that print and online articles differ in terms of news values because of differences in the routines used to produce them. Based on a quantitative automated content analysis of N = 762,095 Dutch news items, we show that online news items are more likely to be follow-up items than print items, and that there are further differences regarding news values like references to persons, the power elite, negativity, and positivity. In order to conduct this large-scale analysis, we developed innovative methods to automatically code a wide range of news values. In particular, this article demonstrates how techniques such as sentiment analysis, named entity recognition, supervised machine learning, and automated queries of external databases can be combined and used to study journalistic content. Possible explanations for the difference found between online and offline news are discussed.
Practices in taste maintenance. The case of Indian diaspora markets
Authors: Gowricharn Ruben
Published: July 2017

This article centres on taste as a crucial driver of consumer behaviour and addresses the issue of how ethnic tastes in hybridized diaspora communities are maintained. The most common answer to this question refers to glocalization, which is briefly described as the adoption (modification) of global elements in the local culture. The article argues that the glocalization of taste occurs through consumer practices, but the concept of practices has been found difficult to apply, as there is no unified definition of it. Accordingly, each time the question of ‘taste for what?’ may be asked. Taste for dresses is something quite different, for example, than the taste for food – and so are the related practices. The concept of taste is therefore specific, as are the operationalized consumer practices. Put differently, taste appears to be a fuzzy concept that must be specified in each case in order to disclose its concrete meaning. Consequently, the article specifies that taste is shaped, maintained and expressed in and through consumer practices that need to display cultural scripts and institutional regularities in the lived culture of the ethnic community. Furthermore, the ethnic lived culture is differentiated from the concepts of ‘everyday ethnicity’, ‘cultural norm images’ and ‘invention of tradition’, detailing the home culture, the community culture and the celebration of traditions, respectively, to highlight how script-driven institutional practices sustain diaspora-related taste. Acknowledging that the specific mode of glocalization varies across diaspora communities, this article elucidates the argument with a case study by Dutch Hindustanis. The article claims that despite being a case study, the maintenance of taste applies to all communities that constitute the Indian diaspora market.
Female Cigarette and Alcohol Consumption as a Short-Term Mating Strategy
Authors: Vincke Eveline
Published: July 2017

In developed countries, the traditional gender gap in youth smoking and drinking is closing. As tobacco and alcohol are more harmful to women than to men, this is an alarming trend. As men are generally more short-term oriented in their sexuality than women, and given that cigarette and alcohol use are still considered masculine behaviors, we explored if female smoking and drinking can function as a short-term mating strategy. By means of a between-subjects experiment ( N = 218), men’s perceptions of female smoking and drinking were studied. The experiment showed that young men perceive women who use cigarettes and alcohol as being more sexually unrestricted. Furthermore, tobacco and (especially) alcohol use brought some short-term attractiveness benefits to women. In short-term mating contexts, drinking enhanced women’s attractiveness, whereas occasional smoking was found equally desirable as not smoking. However, in long-term mating contexts, frequent drinking and all smoking behavior harmed women’s desirability. A follow-up study ( N = 202) confirmed men’s perceptions, showing that female users of tobacco and alcohol are indeed more short-term oriented in their sexuality. Overall, results indicate that female smoking and drinking can operate as a short-term mating strategy.
Self-Resemblance and Social Rejection
Authors: Parsons Carly A. , Jacobson Jill A. , Krupp D. B.
Published: July 2017

Humans perceive and treat self-resembling others in ways that suggest that self-resemblance is a cue of kinship. However, we know little about how individuals respond to treatment by self-resembling others. Here we approach this problem by connecting facial self-resemblance to social rejection. Given that individuals should expect to cooperate with kin, we hypothesized that (1) social inclusion by perceived kin should elicit lesser feelings of rejection and (2) social exclusion by perceived kin should elicit greater feelings of rejection relative to inclusion or exclusion, respectively, by nonkin. To test these hypotheses, we recruited 90 participants to play two games of Cyberball, a virtual ball-tossing game, with separate pairs of ostensible partners. In one game, the ostensible partners were programed to fully include the participants in group play and, in the other game, they were programed to exclude the participants after a few rounds; the order of inclusion and exclusion was counterbalanced across participants. Partner faces were digitally manipulated to be either self- or nonself-resembling, and these conditions were also counterbalanced. Rejection feelings differed significantly as a function of self-resemblance between the inclusion and exclusion conditions, but only for participants who experienced inclusion first. Moreover, for these individuals, inclusion by self-resembling partners led to significantly lesser feelings of rejection than did inclusion by nonself-resembling partners. To explain this effect, we explore potential mechanisms of kin recognition and social rejection. Although nuanced, our results suggest that perceptions of kinship can moderate psychological responses to the actions of others.
Visual Perception of British Women’s Skin Color Distribution in Two Nonindustrialized Societies, the Maasai and the Tsimane’
Authors: Fink Bernhard , Butovskaya Marina , Sorokowski Piotr , Sorokowska Agnieszka , Matts Paul J.
Published: July 2017

In women with lightly pigmented skin in particular, facial skin color homogeneity decreases with age, primarily due to chronic exposure to solar ultraviolet radiation (UVR), leading to a decrease in perceived health and attractiveness. Perception of female skin may be influenced by continuous exposure to, and thus familiarity with, age-related changes in visible skin condition in a given society. Men and women of two traditional societies, the Maasai (Tanzania) and the Tsimane’ (Bolivia), unfamiliar with lighter colored skin, judged images of British women’s facial skin for age, health, and attractiveness. In both samples, images with homogeneous skin color (from the cheeks of younger women) were judged to be younger and healthier and received a stronger preference than corresponding images with heterogeneous skin color (from older women). We suggest that (i) human sensitivity for quality-related information from facial skin color distribution is universal and independent of any known age-dependent variation in skin in a given population and (ii) skin discoloration is universally associated with less positive judgment.
College Students’ Responses to Emotional Anti–Alcohol Abuse Media Messages: Should We Scare or Amuse Them?
Authors: Lee Moon J.
Published: July 2017

This study examined college students’ responses to emotional anti–alcohol abuse media messages (i.e., fear vs. humor appeal) aimed at discouraging heavy/binge drinking. An experiment was conducted with 94 college students. As expected, college students generally expressed higher levels of interest in anti–alcohol abuse media messages when watching fear appeal than those watching humor appeal. However, college binge drinkers who watched the fear appeal reported lower levels of readiness to change their drinking behavior than those who watched the humor appeal. This pattern was the opposite among college nonbinge drinkers, in that college nonbinge drinkers were more likely to say they would change their drinking behaviors when exposed to fear appeal than those who were exposed to humor appeal or those who did not watch any media messages. Conventional fear appeal to scare college binge drinkers seems ineffective and even counterproductive while humor appeal, if well designed, could offer alternative ways to communicate with college binge drinkers.
Data Power in Material Contexts: Introduction
Authors: Kennedy Helen , Bates Jo
Published: July 2017

This short piece introduces the special issue of Television & New Media (TVNM) on data power in material contexts, which brings together papers which analyze the operations of data power across a range of real-world domains. It highlights the increasing connectedness of digital data tracking, aggregation, and analytics across domains that include and move beyond media, as data are increasingly combined and shared across diverse digital spaces. Thus, it connects media and communications scholarship concerned with datafication to debates in other related and overlapping fields, as part of the larger project of building data studies as an interdisciplinary and critical field. It briefly introduces the papers in the special issue, all of which constitute detailed empirical investigations that ground the study of data power in specific, material contexts.
Mechanical characterization of tetraxial textiles
Authors: Ghazimoradi Mehdi , Carvelli Valter , Marchesi Maria Chiara , Frassine Roberto
Published: July 2017

In this paper, the mechanical properties of different tetraxial fabrics are investigated. Fabrics were produced using an innovative loom capable of weaving four threads at the same time with complete discretion of yarn type and count. The experimental investigation deals with in-plane and out-of-plane mechanical testing of tetraxial fabrics, as well as yarns made of four different materials (polyethylene terephthalate, glass, aramid, and basalt). The digital image correlation technique was used to measure the in-plane strain field for both uniaxial and biaxial tensile tests. The extensive experimental campaign allowed for a complete mechanical characterization of this novel fabric architecture including interlacement of different yarns.
Dietary Zinc Supplementation Attenuates Hyperglycemia in db/db Mice
Authors: Simon Sharon F. , Taylor Carla G.
Published: July 2017

Although zinc (Zn) deficiency has been associated with insulin resistance, and altered Zn metabolism (e.g., hyperzincuria, low-normal plasma Zn concentrations) may be present in diabetes, the potential effects of Zn on modulation of insulin action in Type II diabetes have not been established. The objective of this study was to compare the effects of dietary Zn deficiency and Zn supplementation on glycemic control in db/db mice. Weanling db/db mice and lean littermate controls were fed Zn-deficient (3 ppm Zn; dbZD and InZD groups), Zn-adequate control (30 ppm Zn; dbC and InC groups) or Zn-supplemented (300 ppm Zn; dbZS and InZS groups) diets for 6 weeks. Mice were assessed for Zn status, serum and urinary indices of diabetes, and gastrocnemius insulin receptor concentration and tyrosine kinase activity. Fasting serum glucose concentrations were significantly lower in the dbZS group compared with the dbZD group (19.3 ± 2.9 and 27.9 ± 4.1 mM, respectively), whereas the dbC mice had an intermediate value. There was a negative correlation between femur Zn and serum glucose concentrations (r = −0.59 for lean mice, P = 0.007). The dbZS group had higher pancreatic Zn and lower circulating insulin concentrations than dbZC mice. Insulin-stimulated tyrosine kinase activity in gastrocnemius muscle was higher in the db/db genotype, and insulin receptor concentration was not altered. In summary, dietary Zn supplementation attenuated hyperglycemia and hyperinsulinemia in db/db mice, suggesting that the roles of Zn in pancreatic function and peripheral tissue glucose uptake need to be further investigated.
Physiological Role of UCP3 May Be Export of Fatty Acids from Mitochondria When Fatty Acid Oxidation Predominates: An Hypothesis
Authors: Himms-Hagen Jean , Harper Mary-Ellen
Published: July 2017

This hypothesis proposes a physiological role for uncoupling protein-3 (UCP3) in the export of fatty acid anions from muscle and brown adipose tissue (BAT) mitochondria when fatty acids are the predominant substrate being used. It proposes that excess acyl CoA within the mitochondria is hydrolyzed by a mitochondrial acyl CoA thioesterase, yielding fatty acid anion and CoASH. The fatty acid anion is exported to the cytosol by being carried across the inner mitochondrial membrane by UCP3. The CoASH is conserved within the mitochondrion to participate in other reactions for which it is needed during fatty acid oxidation in the β-oxidation cycle and in the tricarboxylic acid cycle. The export of the fatty acid anion thus permits continued rapid fatty acid oxidation in the face of an oversupply. The hypothesis provides a logical explanation for the observed up-regulation of gene expression for UCP3 in muscle when there is a switch to fatty acid oxidation, as during fasting, and in BAT when fatty acid oxidation is stimulated, as during exposure to cold. It provides a plausible physiological role for UCP3 as a transporter protein, not as an uncoupling protein.
Physical and Mechanical Characteristics of Tibias from Transgenic Mice Expressing Mutant Bovine Growth Hormone Genes
Authors: Turner Nancy D. , Knapp Joanne R. , Byers F. Michael , Kopchick John J.
Published: July 2017

Physical and mechanical characteristics of tibia from mice expressing either the M4, M11, or G119K mutant bovine growth hormone (bGH) gene and displaying large, near-normal, or small-size Phenotypes, respectively, were compared to those of non-transgenic, control mice (NTC). Three animals of each strain were euthanized at 28, 38, 48, 58, and 68 days of age. Variables were regressed against age to establish the pattern of change throughout the experiment, and the regression results are presented. Tibias from G119K were shorter (13.1 mm) and lighter (37.3 mg) than those from other strains, and M4 tibias were heavier (87.9 mg) and longer (16.6 mm) at 70 days of age. The ratio of tibia length to body weight suggests longitudinal bone growth was not reduced as much as overall growth in G119K mice. The external and internal dimensions of the G119K tibias were smaller than the other strains whereas the M4 tibias were somewhat larger. Differences in physical dimensions between the NTC and M11 mice did not greatly affect bone mechanical characteristics. Tibias from M4 mice resisted more load at both flexure and breaking compared to the other strains. At 50 days of age, stress at flexure was greater at all ages for G119K mice (12.4 kg/mm2) and was decreased in M4 mice (8.5 kg/mm2). The bGH mutations produce different effects on bone growth and its mechanical characteristics. There also may be differential tissue responsiveness to the mutant bGH analogs, as longitudinal growth was not as affected as empty body growth in the G119K mice. These transgenic mouse strains provide valuable models to study bone growth, formation, and reformation in response to GH regulation, and more importantly, the M4 and G119K mice may serve as a model in which the priorities for GH action on bone vs muscle may be determined.
Cross Talk Between Cyclic Nucleotides and Polyphosphoinositide Hydrolysis, Protein Kinases, and Contraction in Smooth Muscle
Authors: Abdel-Latif Ata A.
Published: July 2017

This article provides an update of a minireview published in 1996 (Abdel-Latif AA. Proc Soc Exp Biol Med 211:163–177, 1996), the purpose of which was to examine in nonvascular smooth muscle the biochemical and functional cross talk between the sympathetic nervous system, which governs the formation of cAMP and muscle relaxation, and the parasympathetic nervous system, which governs the generation of IP3 and diacylglycerol, from the polyphosphoinositides, Ca2+ mobilization, and contraction. This review examines further evidence, both from nonvascular and vascular smooth muscle, for cross talk between the cyclic nucleotides, cAMP and cGMP via their respective protein kinases, and the Ca2+-dependent- and Ca2+-independent-signaling pathways involved in agonist-induced contraction. These include the IP3-Ca2+-CaM- myosin light chain kinase (MLCK) pathway and the Ca2+-independent pathways, including protein kinase C-, MAP kinase-, and Rho-kinase. In addition, MLC phosphorylation and contraction can also be increased by a decrease in myosin phosphatase activity. A summary of the cross talk between the cyclic nucleotides and these signaling pathways was presented. In smooth muscle, there are several targets for cyclic nucleotide Inhibition and consequent relaxation, including the receptor, G proteins, phospholipase C-β1–4 Isoforms, IP3 receptor, Ca2+ mobilization, MLCK, MAP kinase, Rho-kinase, and myosin phosphatase. While significant progress has been made in the past four years on this cross talk, the precise mechanisms underlying the biochemical basis for the cyclic nucleotide inhibition of Ca2+ mobilization and consequently muscle contraction remain to be established. Although it is well established that second-messenger cross talk plays an important role in smooth muscle relaxation, the many sources which exist in smooth muscle for Ca2+ mobilization, coupled with the multiple signaling pathways involved in agonist-induced contraction, contribute appreciably to the difficulties found by many investigators in identifying the targets for cyclic nucleotide inhibition and consequent relaxation. Better methodology and more novel interdisciplinary approaches are required for elucidating the mechanism(s) of cAMP- and cGMP-inhibition of smooth muscle contraction.
Folate, Homocysteine and Neural Tube Defects: An Overview
Authors: Van Der Put Nathalie M.J. , Van Straaten Henny W.M. , Trijbels Frans J.M. , Blom Henk J.
Published: July 2017

Folate administration substantially reduces the risk on neural tube defects (NTD). The interest for studying a disturbed homocysteine (Hcy) metabolism in relation to NTD was raised by the observation of elevated blood Hcy levels in mothers of a NTD child. This observation resulted in the examination of enzymes involved in the folate-dependent Hcy metabolism. Thus far, this has led to the identification of the first and likely a second genetic risk factor for NTD. The C677T and A1298C mutations in the methylenetetrahydrofolate reductase (MTHFR) gene are associated with an increased risk of NTD and cause elevated Hcy concentrations. These levels can be normalized by additional folate intake. Thus, a dysfunctional MTHFR partly explains the observed elevated Hcy levels in women with NTD pregnancies and also, in part, the protective effect of folate on NTD. Although the MTHFR polymorphisms are only moderate risk factors, population-wide they may account for an important part of the observed NTD prevalence.
New Concepts in Characterization of Ischemically Injured Myocardium by MRI
Authors: Saeed Maythem
Published: July 2017

New concepts regarding the assessment of ischemic myocardial injuries have been addressed in this Minireview using magnetic resonance imaging (MRI). MRI, with its different techniques, brings not only anatomic, but also physiologic, information on ischemic heart disease. It has the ability to measure identical parameters in preclinical and clinical studies. MRI techniques provide the ideal package for repeated and noninvasive assessment of myocardial anatomy, viability, perfusion, and function. MR contrast agents can be applied in a variety of ways to improve MRI sensitivity for detecting and assessing ischemically injured myocardium. With MR contrast agents protocol, it becomes possible to identify ischemic, acutely infarcted, and peri-infarcted myocardium in occlusive and reperfused infarctions. Necrosis specific and nonspecific extracellular contrast-enhanced MRI has been used to assess myocardial viability. Contrast-enhanced perfusion MRI can explore the disturbances in large (angiography) and small coronary arteries (myocardial perfusion) as the underlying cause of myocardial dysfunction. Perfusion MRI has been used to measure myocardial perfusion (ml/min/g) and to demonstrate the difference in transmural myocardial blood flow. Information on no-reflow phenomenon is derived from dynamic changes in regional signal intensity after bolus injection of MR contrast agents. Another development is the near future availability of blood pool MR contrast agents. These agents are able to assess microvascular permeability and integrity and are advantageous in MR angiography (MRA) due to their persistence in the blood. Non-contrast-enhanced MRI such as cine MRI at rest/stress, sodium MRI, and MR spectroscopy also have the potential to noninvasively assess myocardial viability in patients. Futuristic applications for MRI in the heart will focus on identifying coronary artery disease at an early stage and the beneficial effects of new therapeutic agents such as intra-arterial gene therapy. MR techniques will have great future in the drug discovery process and in testing the effects of drugs on myocardial biochemistry, physiology, and morphology. Molecular imaging is going to bloom in this decade.
The Role of n-3 Fatty Acids in Gestation and Parturition
Authors: Allen Kenneth G.D. , Harris Mary A.
Published: July 2017

Preterm birth is the most common cause of low infant birth weight and infant morbidity and mortality. Evidence from human and animal studies indicates that essential fatty acids of both the n-3 and n-6 series, and their eicosanoid metabolites, play important and modifiable roles in gestational duration and parturition, and n-3 fatty acid intake during pregnancy may be inadequate. Prostaglandins (PG) of the 2-series are involved in parturition and connective tissue remodeling associated with cervical maturation and rupture of membranes. In the absence of infections, preterm birth is characterized by lower reproductive tissue PG production and decreased inducible cyclooxygenase expression. Women who deliver prematurely have increased pools of n-6 fatty acid and decreased n-3 fatty acids, despite the lower PG production. Several human pregnancy supplementation trials with n-3 fatty acids have shown a significant reduction in the incidence of premature deliver and increased birth weight associated with increased gestational duration. Supplementation with long chain n-3 fatty acids such as docosahexaenoic acid may be useful in prolonging the duration of gestation in some high-risk pregnancies. Evidence presented in this review is discussed in terms of the roles of dietary n-3 and n-6 fatty acids in gestation and parturition, mechanisms by which they may influence gestational duration and the human trials suggesting that increased dietary long-chain n-3 fatty acids decrease the incidence of premature delivery.
Effects of Selected Minerals on Leptin Secretion in Streptozotocin-lnduced Hyperglycemic Mice
Authors: Chen Ming-Der , Yang Vivian C. , Alexander Paul S. , Lin Pi-Yao , Song Yuh-Min
Published: July 2017

The effects of lithium, magnesium, vanadate, and zinc on leptinemia and leptin secretion by adipose tissue were investigated in streptozotocin- (STZ) induced hyperglycemic mice. After the administration of studied minerals in drinking water for 4 weeks, fasting serum leptin concentrations were elevated, accompanied by normoglycemia in STZ-injected mice, regardless which mineral was provided (P < 0.05). However, the in vitro administration of lithium, magnesium, and vanadate did not significantly influence the leptin secretion of adipose tissue. A low zinc treatment (0.1 mM) augmented, whereas both a pharmacological treatment of zinc (1 mM) and zinc depletion (1 mM TPEN) attenuated, leptin secretion (P < 0.05). The present study shows that STZ-induced hyperglycemic mice have hypoleptinemia and reduced leptin secretion by adipose tissue. Moreover, these defects can be improved by a moderate zinc administration.
Reduced Oxygen Tension Increases Atrial Natriuretic Peptide Release from Atrial Cardiocytes
Authors: Klinger James R. , Pietras Linda , Warburton Rod , Hill Nicholas S.
Published: July 2017

To test the hypothesis that reduced oxygen tension stimulates cardiac atrial natriuretic peptide (ANP) secretion, we measured ANP release and expression in neonatal rat atrial and ventricular cardiac myocytes exposed to 45 min and 3, 6, and 24 hr of 3% or 21% oxygen. In atrial cardiocytes, the percentage of increase in culture media ANP concentration from baseline was greater in ceils exposed to 3% than in cells exposed to 21% oxygen after 3 hr (814% ± 52% vs. 567% ± 33%, P < 0.05) and 6 hr of exposure (1639% ± 91% vs. 1155% ± 73%, P < 0.05). No differences in the percentage of increase in culture media ANP concentration was seen at 45 min (284% ± 27% vs. 201% ± 16%, P = NS) or 24 hr (2499% ± 250% vs. 2426% ± 195%). There was a significant increase in cellular ANP content between 3 and 24 hr in atrial cardiocytes exposed to 21% oxygen (105% ± 40% vs. 296% ± 60%, P < 0.05), but not In atrial cardiocytes exposed to 3% oxygen (118% ± 20% vs. 180% ± 26%, P = NS). Steady-state ANP mRNA levels in atrial cardiocytes were not affected by oxygen tension. In ventricular cardiocytes, oxygen tension did not affect ANP secretion, cellular ANP content, or steady-state ANP mRNA levels. We conclude that reduced oxygen tension increases release of ANP from atrial, but not ventricular cardiocytes and that this mechanism may contribute to the elevation in plasma ANP seen during acute hypoxia.
Acute Pancreatitis Signals Activation of Apoptosis-Associated and Survival Genes in Mice
Authors: Gomez Guillermo , Lee Heung-Man , He Qin , Englander Ella W. , Uchida Tatsuo , Greeley George H.
Published: July 2017

In experimental models of acute pancreatitis (AP), acinar cell death occurs by both necrosis and programmed cell death or apoptosis. Apoptosis is an active form of cell death associated with a tightly regulated expression of gene products that are either pro- or antiapoptotic. The aim of this study was to characterize pancreatic mRNA levels by Northern blotting analysis of apoptosis-associated genes used during the course of cerulein-induced AP in mice. Histone H3 mRNA levels were also examined as an indicator of cell proliferation. Acinar cell apoptosis was confirmed histologically. The findings show that AP modifies pancreatic mRNA levels of both pro- and antiapoptotic genes simultaneously. Pancreatic bclXL, bax, and p53 mRNA levels increased significantly in a temporal fashion during induction of AP. Pancreatic bcl-2 mRNA levels were unchanged during AP. Pancreatic mRNA levels of insulin-like growth factor-1 (IGF-1), a mitogen and cell survival factor, and its receptor (IGF-1R) also increased in a temporal fashion during induction of AP. In summary, this study indicates that acinar cell death during cerulein-induced AP in mice can occur by the apoptotic pathway. Since factors promoting and antagonistic for cell survival are activated simultaneously, regulation of acinar cell survival appears complex and dynamic during AP.
BMP-4 and BMP-6 Involvement in the Osteogenic Properties of the HeLa Cell Line
Authors: Kochanowska Iwona Ewa , Wlodarski Krzysztof , Wojtowicz Andrzej , Kinsner Agnieszka , Ostrowski Kazimierz
Published: July 2017

The heterotopically induced ossicles are used in our research on bone tissue. The ossicles are formed in the thigh muscle of BALB/c mice under the influence of injected suspension of 3 × 106 HeLa cells. We postulate that the mechanism of bone induction is based on the secretion of bone morphogenetic proteins BMP-4 and BMP-6 by the grafted HeLa cells. This was proved by the use of specific immunohistochemical reaction and Western blots of conditioned culture medium. It seems that HeLa cells secrete BMPs continuously into the culture medium, even without contact with the mice muscle tissue, were induction of bone tissue is observed.
Nitric Oxide as the Mediator of the Antiosteoporotic Actions of Estrogen, Statins, and Essential Fatty Acids
Authors: Das Undurti N.
Published: July 2017

Estrogen, statins, and essential fatty acids and their metabolites can prevent osteoporosis. However, it is not certain how these three structurally different agents can have the same beneficial action. It is suggested that all three, in addition to their other modes of action in the prevention of osteoporosis, have the ability to augment constitutional (or endothelial) nitric oxide generation, which is known to be beneficial in osteoporosis. If so, it will be interesting to study whether nitric oxide donors and/or nitric oxide precursors can be given together with estrogen, statins, or essential fatty acids to potentiate their benefit in osteoporosis.
An Aged Mouse Model for RSV Infection and Diminished CD8+ CTL Responses
Authors: Zhang Yongxin , Wang Ying , Gilmore Xyanthine , Xu Keyi , Wyde Philip R. , Mbawuike Innocent N.
Published: July 2017

Recent studies indicate that respiratory syncytial virus (RSV), like influenza, causes significant morbidity and mortality among elderly persons. There are currently no animal models to study the effects of aging on RSV disease and immunity. This manuscript provides an initial description of such a model. Aged and young BALB/c mice (22-24 and 2-4 months, respectively) were infected with 104 TCID50 of RSV A2. RSV was detected by culture in lung and nose wash specimens obtained 4-6 days following infection at a slightly higher titer in old mice in comparison with young mice. RT-PCR assay detected RSV in the lungs and nose washes of all mice on 4, 8, and 21 days postinoculation, with only a slightly less frequency in young mice. Splenic lymphocytes from old mice exhibited significantly lower RSV-specif ic MHC class I-restricted CD8+ CTL responses (P < 0.01-0001), and reduced IFN-production (P < 0.03) than young mice. Conversely, IL-4 production was somewhat elevated in old mice. These results demonstrate diminished RSV virus-specific CD8+ CTL responses and IFN-γ production in old mice in comparison with young. It is speculated that the deficient RSV-specific CTL responses may account for the Increased morbidity and mortality from RSV Infections in elderly persons. Although detailed hlstopathological, virological, and immunological analyses are incomplete at present, the old BALB/c RSV infection model described provides an opportunity to evaluate the role of CD8+ CTL and cytokines in RSV disease in aging.