bluuebunny/crossref_metadata_2025 huggingface dataset:
Elevation and Soil Properties Determine Community Composition, but Not Vascular Plant Richness in Tropical Andean Roadside
Authors: Guzmán Patricio , Benítez Ángel , Carrión-Paladines Vinicio , Salinas Paul , Cumbicus Nixon
Published: April 2022

Roadsides are common ecosystems worldwide, with specific environmental characteristics and multiple effects on plant diversity. As such, they represent examples of highly dynamic anthropogenic ecosystems. Our objective was to assess patterns of vascular species diversity in response to elevation and soil characteristics on a roadside in the Andean mountains of Ecuador. The study area was located in the southern Ecuadorian Andes, at five elevations ± 400 m a.s.l. (2600, 2200, 1800, 1400 and 1000 m a.s.l.), where we recorded species richness and abundance in transects perpendicular to the road. The effects of elevation and soil characteristics on species abundance and richness were analyzed using generalized linear mixed models (GLMMs), while species composition was assessed with a non-metric multidimensional scaling analysis (NMDS) and its relationship to environmental variables. We used indicator species analyses (ISA) to identify which species significantly characterized specific elevation and soil factors from primary succession for restoration processes at the roadside. Although elevation and soil characteristics do not condition vascular species richness, the composition is more similar at elevations E1 and E2 (2600 m and 2200 m a.s.l.), differing from low elevations E4 and E5 (1400 m and 1000 m a.s.l.), which in turn are more similar to each other, while intermediate elevation E3 is similar to the highest and lowest elevations. Soil variables that limited plant communities were pH, bulk density (gr/cm3), silt (%), and sand (%) contents. The indicator species showed a preference for specific environmental and soil condition requirements associated with the different microhabitats and, thus, can be suggested for potential use in roadside revegetation processes in tropical areas. These results can help decision-makers in the implementation of biodiversity conservation and roadside environmental restoration projects in areas of Andean mountain ecosystems which have been affected by the construction of road infrastructure.
Construction of a ceRNA Network and Comprehensive Analysis of lncRNA in Hepatocellular Carcinoma
Authors: Wang Lin , Zhao Jun , Zhu Cancan , Yang Ke , Zhu Ling , Liu Yong
Published: April 2022

To explore the RNA biomolecular marker associated with hepatocellular carcinoma (HCC) prognosis, we constructed a regulatory network of competitive endogenous RNAs (ceRNAs), which provides favorable conditions for the early diagnosis, prognostic monitoring, and personalized treatment of HCC. In this study, the differentially expressed genes (DEGs) of patients with HCC were obtained from the Gene Expression Omnibus. We identified 574 upregulated genes and 274 downregulated genes relevant to HCC occurrence (p < 0.05). Subsequently, we constructed the protein–protein interaction (PPI) network using these DEGs and identified the hub genes from the PPI. We then determined the expression and prognostic values of the hub genes from the GEPIA and Kaplan–Meier plotter databases. After the upstream microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) were respectively identified by miRTarBase and miRNet, we validated the expression of the key miRNAs in the serum using qPCR experiments. Moreover, we identified a two-lncRNA (LINC01184 and ADORA2A-AS1) signature from the upstream lncRNA that effectively predicted overall survival and had promotive effects for HCC. To verify the clinical significance of the signature, we validated the expression of the lncRNA in HCC tissues. Finally, we discovered and identified four mRNAs, four miRNAs, and five lncRNAs associated with the prognosis of HCC and constructed a new ceRNA regulatory network, which will be beneficial for the accurate diagnosis and treatment of HCC.
Transcriptome Analysis of Fusarium Root-Rot-Resistant and -Susceptible Alfalfa (Medicago sativa L.) Plants during Plant–Pathogen Interactions
Authors: Zhang Wenyu , Wang Zicheng , Dan Zhencuo , Zhang Lixia , Xu Ming , Yang Guofeng , Chai Maofeng , Li Zhenyi , Xie Hongli , Cong Lili
Published: April 2022

Alfalfa (Medicago sativa L.) is a perennial leguminous forage cultivated globally. Fusarium spp.-induced root rot is a chronic and devastating disease affecting alfalfa that occurs in most production fields. Studying the disease resistance regulatory network and investigating the key genes involved in plant–pathogen resistance can provide vital information for breeding alfalfa that are resistant to Fusarium spp. In this study, a resistant and susceptible clonal line of alfalfa was inoculated with Fusarium proliferatum L1 and sampled at 24 h, 48 h, 72 h, and 7 d post-inoculation for RNA-seq analysis. Among the differentially expressed genes (DEGs) detected between the two clonal lines at the four time points after inoculation, approximately 81.8% were detected at 24 h and 7 d after inoculation. Many DEGs in the two inoculated clonal lines participated in PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI) mechanisms. In addition, transcription factor families such as bHLH, SBP, AP2, WRKY, and MYB were detected in response to infection. These results are an important supplement to the few existing studies on the resistance regulatory network of alfalfa against Fusarium root rot and will help to understand the evolution of host–pathogen interactions.
A Common Missense Variant Causing Factor XI Deficiency and Increased Bleeding Tendency in Maine Coon Cats
Authors: Kuder Henrike , Dickeson S. Kent , Brooks Marjory B. , Kehl Alexandra , Müller Elisabeth , Gailani David , Giger Urs
Published: April 2022

Hereditary factor XI (FXI) deficiency is characterized as an autosomal mild to moderate coagulopathy in humans and domestic animals. Coagulation testing revealed FXI deficiency in a core family of Maine Coon cats (MCCs) in the United States. Factor XI-deficient MCCs were homozygous for a guanine to adenine transition resulting in a methionine substitution for the highly conserved valine-516 in the FXI catalytic domain. Immunoblots detected FXI of normal size and quantity in plasmas of MCCs homozygous for V516M. Some FXI-deficient MCCs experienced excessive post-operative/traumatic bleeding. Screening of 263 MCCs in Europe revealed a mutant allele frequency of 0.232 (23.2%). However, V516M was not found among 100 cats of other breeds. Recombinant feline FXI-M516 (fFXI-M516) expressed ~4% of the activity of wild-type fFXI-V516 in plasma clotting assays. Furthermore, fFXIa-M516 cleaved the chromogenic substrate S-2366 with ~4.3-fold lower catalytic efficacy (kcat/Km) than fFXIa-V516, supporting a conformational alteration of the protease active site. The rate of FIX activation by fFXIa-M516 was reduced >3-fold compared with fFXIa-V516. The common missense variant FXI-V516M causes a cross-reactive material positive FXI deficiency in MCCs that is associated with mild-moderate bleeding tendencies. Given the prevalence of the variant in MCCs, genotyping is recommended prior to invasive procedures or breeding.
Methodological Approach for the Study of Historical Centres of High Architectural Value Affected by Geo-Hydrological Hazards: The Case of Lanciano (Abruzzo Region—Central Italy)
Authors: Sciarra Nicola , Mangifesta Massimo , Carabba Luigi , Mischiatti Luigina
Published: April 2022

The study of geo-hydrological problems in urban contexts of considerable historical importance plays an extremely interesting role in the safeguarding of architectural and artistic assets of great value. The need to guarantee the conservation of monumental heritage is an ethical and moral requirement that new generations have a duty to support. Operating in urbanised contexts is extremely difficult, due to the presence of infrastructures and underground services that prevent the execution of classical surveys and prospecting. The technologies currently available, however, allow us to also investigate the subsoil in a non-destructive way and to control the evolution of active natural phenomena in a continuous and automated way with remote-sensing techniques. The methodological approach consists of the development of a series of cognitive investigations, aimed at identifying the elements of weakness of the soil system, so as to be able to subsequently undertake the most appropriate decisions for the reduction of geo-hydrological risks. The case here analysed concerns Lanciano city (Central Italy), famous for its pre-Roman origins, that was affected by a violent storm in the summer of 2018. This event devastated the inhabited Centre with flooding of all the neighbourhoods and the collapse of parts of buildings. For this reason, direct and indirect geognostic investigations were carried out within the Historical Centre, which is of considerable architectural value, and an important monitoring system was installed. The actual geo-hydrological hazard was planned using 3D numerical modelling to define the hydraulic and deformational behaviour of the subsoil. Comparison between the modelling performed and the monitoring data acquired has allowed us to understand the complex behaviour of the subsoil and the subsidence mechanisms of the Historic Centre.
An Analysis of Waiting Time for Emergency Treatment and Optimal Allocation of Nursing Manpower
Authors: Liao Pei-Hung , Chu William , Ho Chen-Shie
Published: April 2022

Objective: Emergency care is the frontline of the healthcare system. Taiwanese typically seek emergency care when suffering from an acute or unknown illness, which leads to a large number of emergency patients and the related misallocation of nursing manpower, and the excessive workloads of emergency service providers have become serious issues for Taiwan’s medical institutions. Participants: This study conducted purposive sampling and recruited patients and nursing staffs from the emergency room of a medical center in New Taipei City as the research participants. Methods: This study applied the queueing theory and the derived optimal model to solve the problems of excessive workloads for emergency service providers and misallocation of nursing manpower, in an attempt to provide decision makers with more flexible resource allocation and process improvement suggestions. Results: This study analyzed the causes of emergency service overload and identified solutions for improving nursing manpower utilization. Conclusions: A wait-time model and the queueing theory were used to determine resource parameters for the optimal allocation of patient waiting times and to develop the best model for estimating nursing manpower.
A Systematic Review of Physical Activity Intervention Programs in ASEAN Countries: Efficacy and Future Directions
Authors: Lee Yi-Shin , Chia Michael , Komar John
Published: April 2022

A systematic review was conducted on the efficacy of interventions to improve physical activity. PubMed, Scopus and Web of Science were scanned for eligible studies published from 1978 to August 2021, resulting in a total of 52 relevant studies for review. The Downs and Black checklist was used as a quality assessment ool for a risk of bias assessment. The 52 studies were then broadly categorised into three major approach types: informational, behavioural and/or social, as well as direct. Within each major approach, studies were further sub-categorised into more specific intervention types before being assessed for their efficacy and applicability. Overall, the intervention types that seemed to be the most efficacious in increasing physical activity levels were those that involved home-based information provision, community-wide campaigns, incentivised change, individually adapted health behaviour programs, family-based social support interventions and the provision of self-monitoring tools. However, the results must be interpreted holistically, as many of the successful interventions included more than one approach type and success is likely contingent on effectively addressing several concurrent facets. The systematic review is registered on PROSPERO. Registration number: 282752.
Prevention of Depression and Anxiety in Subclinical Adolescents: Effects of a Transdiagnostic Internet-Delivered CBT Program
Authors: Schmitt Julia C. , Valiente Rosa M. , García-Escalera Julia , Arnáez Sandra , Espinosa Victoria , Sandín Bonifacio , Chorot Paloma
Published: April 2022

Anxiety and depressive symptoms are common problems in adolescence that could be addressed by means of preventive interventions. Even though transdiagnostic cognitive behavior therapy (T-CBT) is potentially an ideal strategy to deal with anxiety and depression, it has rarely been used for preventive purposes. In addition, so far, no study has used internet-delivered T-CBT to prevent anxiety and depression in adolescents. This study aimed to examine the utility of AMTE, an internet-delivered T-CBT program, for the indicated prevention of anxiety and depression in adolescents. AMTE was applied to 30 adolescents (56.7% females, age range = 12–18 years, Mage = 14.00, SDage = 1.89) who showed subclinical symptoms of anxiety and/or depression. Participants were assessed at pre- and post-treatment and follow-up (3 months). We found that after the program, the symptoms of self-reported anxiety and depression, clinician-rated symptom severity, and self-reported and parent-reported severity of the main problems had significantly improved. In addition, there were significant improvements in anxiety sensitivity and emotional avoidance. Finally, we found high feasibility and acceptability of the program. AMTE is feasible and potentially effective for the indicated prevention of anxiety and depression as well as of clinical transdiagnostic factors, in adolescents.
Efficacy of Prenatal Yoga in the Treatment of Depression and Anxiety during Pregnancy: A Systematic Review and Meta-Analysis
Authors: Lin I-Hui , Huang Chueh-Yi , Chou Shih-Hsiang , Shih Chia-Lung
Published: April 2022

Women commonly suffer from depression during pregnancy. For reducing depression, yoga seems to be more suitable for pregnant women than other physical activities because of its low exercise intensity. The objective of this study was to assess the efficacy of prenatal yoga on the treatment of depression during pregnancy. Three electronic databases were searched for relevant articles from their inception to May 2021, including PubMed, Cochrane Library, and ScienceDirect. Pre- and post-test outcomes were adopted to estimate standardized mean difference with a 95% confidence interval for assessing the efficacy of yoga. Heterogeneity among articles was detected using I2 value. A total of 13 articles that contained 379 subjects were included for meta-analysis. No significant improvement in depression scores after practicing yoga was observed for women without depression (p = 0.09) but significant improvement was observed for women with depression (p = 0.001). Although significant improvement in anxiety scores after yoga was observed for women without depression (p = 0.02), the results of the sensitivity analysis were not consistent, while significant improvement in anxiety scores after yoga was also observed for women with depression (p < 0.00001). The current evidence has suggested that yoga had significant improvement in depression and anxiety scores in pregnant women with depression. However, the level of evidence of this study was not high. More articles with high levels of evidence should be conducted to confirm our conclusion in the future.
Differences in Overweight or Obesity, Changes in Dietary Habits after Studying Abroad and Sleep Quality by Acculturative Degree among Asian Foreign Students: A Cross Sectional Pilot Study
Authors: Doo Miae , Wang Chunyang
Published: April 2022

The number of foreign students is increasing worldwide, and they suffer from acculturation to different environments or cultures. This pilot study examined the difference in overweight or obesity, changes in dietary habits after studying abroad and sleep quality according to acculturative degree among 225 Asian foreign students in South Korea. Most subjects (61.8%) experienced a low acculturative degree. The change in dietary habits after studying abroad showed a significant difference according to the acculturative degree (p < 0.001); however, there were no differences observed in sleep quality (p = 0.090) and prevalence of overweight or obesity according to acculturative degree (p = 0.101). Interestingly, a difference in the risk for being overweight or obese by sleep quality after being stratified into groups according to acculturative degree was observed. Among the groups with a low acculturative degree, subjects reporting poor sleep quality had a 2.875-fold (95% CI = 1.167–7.080) higher risk of being overweight or obese than those reporting good sleep quality. However, the risk of being overweight or obese was not different among the high acculturative group regardless of their sleep quality. The results showed that the degree of acculturation could influence the risk of being overweight or obese according to sleep quality among Asian foreign students.
Changes in Upper-Body Muscular Strength and Power in Paralympic Swimmers: Effects of Training Confinement during the COVID-19 Pandemic
Authors: Cavaggioni Luca , Rossi Alessio , Tosin Massimiliano , Scurati Raffaele , Michielon Giovanni , Alberti Giampietro , Merati Giampiero , Formenti Damiano , Trecroci Athos
Published: April 2022

The aim of this case series was to evaluate the effectiveness of a dry-land home-training program conducted during the COVID-19 pandemic period in Paralympic swimmers. Previous evidence showed the importance of muscular strength and power training for Paralympic swimmers due to the positive relationship between severity of impairment, swimming technique and biomechanics parameters. Specifically, we aimed to analyze: (i) the effects of a customized training regime conducted pre, during and post restrictions on upper-body muscular strength and power (one repetition maximum, mean propulsive velocity, and mean relative propulsive power) compared to a regular gym-based program; (ii) the associations between mean propulsive velocity and load during two upper body exercises in order to estimate the one repetition maximum. Four elite Paralympic swimmers were retrospectively analyzed in upper-body muscular strength, mean propulsive velocity and mean relative propulsive power in bench press and lat pull-down exercises at three time points: T0 (prior the Lockdown period), T1 (immediately after the Lockdown confinement), T2 (sixteen weeks after returning to gym training). Our findings suggest a very likely decrement in one repetition maximum, mean propulsive velocity, and mean relative propulsive power during the Lockdown period compared with the T0 period with a subsequent very likely increment in one repetition maximum after returning to gym training (T2) compared with the lockdown period (T0). Conversely, mean relative propulsive power showed an unclear improvement in all athletes in T2 compared with T1. These results were also corroborated by the Friedman’s test followed by the Dunn’s pairwise comparison that mainly showed a decrement from T0 to T1 (p < 0.05). At the same time, it appears that muscle strength and power could be rapidly restored close to the pre-lockdown levels following an adequate training program in the gym, albeit without significance (p > 0.05). Finally, the close relationship between mean propulsive velocity and load in bench press and lat pull-down exercises was also confirmed in para swimming, making a possible estimation of one repetition maximum.
Frequency of Asbestos Exposure and Histological Subtype of Ovarian Carcinoma
Authors: Vidican Pauline , Perol Olivia , Fevotte Joëlle , Fort Emmanuel , Treilleux Isabelle , Belladame Elodie , Zavadil Jiri , Fervers Béatrice , Charbotel Barbara
Published: April 2022

The International Agency for Research on Cancer established a causal link between asbestos exposure and ovarian cancer. However, the exposure frequency and histological characteristics of asbestos-associated ovarian cancers remain to be investigated in detail. This multicenter case–case study assessed the asbestos exposure in ovarian carcinoma (OC) patients, alongside its association with histological subtype. Women were recruited in four hospitals in Lyon, France. Histological reports were reviewed by a pathologist. Patient and family members’ data were collected by phone-based questionnaires. Asbestos exposure was defined as direct (occupational and environmental) and indirect (via parents, partners, and children). An industrial hygienist assessed the probability and level of exposure. The 254 enrolled patients (mean age 60 years) reported having an average of 2.3 different jobs (mean working duration 29 years). The prevalence of direct and indirect asbestos exposure was 13% (mean exposure duration 11 years) and 46%, respectively. High-grade serous carcinoma accounted for 73% of all OCs and 82% of histological subtypes in women with direct exposure. After adjustment on a familial history of OC, no significant associations between asbestos exposure (direct and/or indirect) and high-grade serous carcinoma were found. Women with OC had a high prevalence of asbestos exposure. Establishing risk profiles, as reported here, is important in facilitating compensation for asbestos-related OCs and for the surveillance of women at risk.
Modeling and Predicting Pulmonary Tuberculosis Incidence and Its Association with Air Pollution and Meteorological Factors Using an ARIMAX Model: An Ecological Study in Ningbo of China
Authors: Chen Yun-Peng , Liu Le-Fan , Che Yang , Huang Jing , Li Guo-Xing , Sang Guo-Xin , Xuan Zhi-Qiang , He Tian-Feng
Published: April 2022

The autoregressive integrated moving average with exogenous regressors (ARIMAX) modeling studies of pulmonary tuberculosis (PTB) are still rare. This study aims to explore whether incorporating air pollution and meteorological factors can improve the performance of a time series model in predicting PTB. We collected the monthly incidence of PTB, records of six air pollutants and six meteorological factors in Ningbo of China from January 2015 to December 2019. Then, we constructed the ARIMA, univariate ARIMAX, and multivariate ARIMAX models. The ARIMAX model incorporated ambient factors, while the ARIMA model did not. After prewhitening, the cross-correlation analysis showed that PTB incidence was related to air pollution and meteorological factors with a lag effect. Air pollution and meteorological factors also had a correlation. We found that the multivariate ARIMAX model incorporating both the ozone with 0-month lag and the atmospheric pressure with 11-month lag had the best performance for predicting the incidence of PTB in 2019, with the lowest fitted mean absolute percentage error (MAPE) of 2.9097% and test MAPE of 9.2643%. However, ARIMAX has limited improvement in prediction accuracy compared with the ARIMA model. Our study also suggests the role of protecting the environment and reducing pollutants in controlling PTB and other infectious diseases.
Protecting Human Health from Climate Change: Legal Obligations and Avenues of Redress under International Law
Authors: Wewerinke-Singh Margaretha , Doebbler Curtis
Published: April 2022

In this contribution, we explore how human health can be protected from climate change and its adverse effects by reliance on States’ obligations under international law. We achieved this by reviewing the principal legal instruments that establish the right to health, as well as those that recognize that climate change has an adverse impact on health (Part II). We then examine the means of redress that may be available to those whose human right to health has been interfered with or violated because of climate change (Part III). Finally, we draw some conclusions as to the current effectiveness and future direction of these developments.
Codesign and Feasibility Testing of a Tool to Evaluate Overweight and Obesity Apps
Authors: Puigdomènech Elisa , Robles Noemi , Balfegó Mariona , Cuatrecasas Guillem , Zamora Alberto , Saigí-Rubió Francesc , Paluzié Guillem , Moharra Montserrat , Carrion Carme
Published: April 2022

Background: Digital health interventions and mobile technologies can help to reduce the rates of obesity and overweight conditions. Although weight management apps are widely used, they usually lack professional content and evaluation, so the quality of these apps cannot be guaranteed. The EVALAPPS project aims to design and validate a tool to assess the safety and effectiveness of health-related apps whose main goal is to manage and prevent obesity and overweight conditions. Objective: The aim of this paper is two-fold: (a) to co-create and codesign the EVALAPPS assessment tool and (b) to pilot its feasibility among overweight and obese individuals that use weight control apps. Methods: A mixed-methods approach was used. A multidisciplinary team (n = 12) participated in a co-creation workshop to provide proposals and inputs about the look and feel of the content, usability aspects, appearance, sections, and main features of the EVALAPPS tool. The tool was tested for its feasibility among 31 overweight and obese individuals, attending the CP Endocrinologia i Nutrició SL Clinic for the first time. Participants were asked to use a specific weight control app [Yazio (YAZIO GmbH, Erfurt, Germany), My FitnessPal (MyFitnessPal, Austin, TX, USA) or MyPlate (MyPlate, Santa Monica, CA, USA)] for two weeks and then evaluate them by using the EVALAPPS (EVALAPPS, David Ganyan, Barcelona, Spain) (June 2020, David Ganyan, Barcelona, Spain) tool. Seven participants were phone interviewed to gain more insight into the use of the EVALAPPS tool. Results: The co-creation workshop allowed conceptualizing the EVALAPPS tool. The feasibility study showed that all criteria from the Usability and Functionality dimensions had valid answers, while Reliability, Security, Privacy, and Health indicators were the dimensions with less valid answers. In all three apps, the dimension with the highest score was Usability/functionality, followed by app purpose. Clinical effectiveness and Development were the dimensions with the lowest scores in all three tested weight control apps. Conclusions: The participation of the multidisciplinary team and end-users in the conceptualization and testing of a tool to assess health apps was feasible and relevant for the usability of the tool.
Molecular Epidemiology of Antibiotic Resistance Genes and Virulence Factors in Multidrug-Resistant Escherichia coli Isolated from Rodents, Humans, Chicken, and Household Soils in Karatu, Northern Tanzania
Authors: Sonola Valery Silvery , Katakweba Abdul , Misinzo Gerald , Matee Mecky Isaac
Published: April 2022

The interaction of rodents with humans and chicken in the household environment can facilitate transmission of multidrug-resistant (MDR) Escherichia coli (E. coli), causing infections that are difficult to treat. We investigated the presence of genes encoded for carbapenem, extended spectrum beta-lactamases (ESBL), tetracycline and quinolones resistance, and virulence among 50 MDR E. coli isolated from human (n = 14), chicken (n = 12), rodent (n = 10), and soil (n = 14) samples using multiplex polymerase chain reaction (PCR). Overall, the antimicrobial resistance genes (ARGs) detected were: blaTEM 23/50 (46%), blaCTX-M 13/50 (26%), tetA 23/50 (46%), tetB 7/50 (14%), qnrA 12/50 (24%), qnrB 4/50 (8%), blaOXA-48 6/50 (12%), and blaKPC 3/50 (6%), while blaIMP, blaVIM, and blaNDM-1 were not found. The virulence genes (VGs) found were: ompA 36/50 (72%), traT 13/50 (26%), east 9/50 (18%), bfp 5/50 (10%), eae 1/50 (2%), and stx-1 2/50 (4%), while hlyA and cnf genes were not detected. Resistance (blaTEM, blaCTX-M, blaSHV, tetA, tetB, and qnrA) and virulence (traT) genes were found in all sample sources while stx-1 and eae were only found in chicken and rodent isolates, respectively. Tetracycline resistance phenotypes correlated with genotypes tetA (r = 0.94), tetB (r = 0.90), blaKPC (r = 0.90; blaOXA-48 (r = 0.89), and qnrA (r = 0.96). ESBL resistance was correlated with genotypes blaKPC (r = 0.93), blaOXA-48 (r = 0.90), and qnrA (r = 0.96) resistance. Positive correlations were observed between resistance and virulence genes: qnrB and bfp (r = 0.63) also blaTEM, and traT (r = 0.51). Principal component analysis (PCA) indicated that tetA, tetB, blaTEM, blaCTX-M, qnrA, and qnrB genes contributed to tetracycline, cefotaxime, and quinolone resistance, respectively. While traT stx-1, bfp, ompA, east, and eae genes contributed to virulence of MDR E. coli isolates. The PCA ellipses show that isolates from rodents had more ARGs and virulence genes compared to those isolated from chicken, soil, and humans.
Virtual Reality (VR) Technology for Treatment of Mental Health Problems during COVID-19: A Systematic Review
Authors: Hatta Muhammad Hizri , Sidi Hatta , Siew Koon Chong , Che Roos Nur Aishah , Sharip Shalisah , Abdul Samad Farah Deena , Wan Xi Ong , Das Srijit , Mohamed Saini Suriati
Published: April 2022

There was a surge in psychological distress and emotional burnout during the COVID-19 pandemic. Virtual reality (VR) is helpful as a psychological intervention whilst maintaining physical or social distancing. The present systematic review assessed the role of VR as a psychological intervention tool for mental health problems during the COVID-19 pandemic. We conducted a systematic review that followed the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. This study used the search-related terms: (Virtual reality OR simulated-3D-environment OR VR) AND (covid! or corona!) AND (mental* OR psychologic* OR well* OR health*) AND (intervention) on six databases, i.e., MEDLINE, PsycINFO, Ovid Medline, EMBASE, ACM digital library, and Cochrane Central Register of Controlled Trials (CENTRAL) from the inception date until 23 June 2021. We finally included four studies in the systematic review out of the 379 references imported for screening. These studies reveal that VR is beneficial as a psychological tool for intervention in individuals with mental health problems. Immersed in the telepresence, interacting in a 3-D format compared to a 2-D layout, having a sense of enjoyment and engagement, activating an affective-motivational state, “escaping” to a virtual from the real world are pivotal faucets of VR as a psychological tool for intervention.
The Gamblification of Investing: How a New Generation of Investors Is Being Born to Lose
Authors: Newall Philip W. S. , Weiss-Cohen Leonardo
Published: April 2022

Investing and gambling share key features, in that both involve risk, the coming together of two or more people, and both are voluntary activities. However, investing is generally a much better way than gambling for the average person to make long-run profits. This paper reviews evidence on two types of “gamblified” investment products where this advantage does not hold for investing: high-frequency stock trading and high-risk derivatives. This review defines a gamblified investment product as one that leads most investors to lose, that attracts people at risk of experiencing gambling-related harm, and that utilizes product design principles from gambling (either by encouraging a high frequency of use or by providing the allure of big lottery-like wins). The gamblification of investing produces novel challenges for the regulation of both financial markets and gambling.
A Study of Carbon Emission Efficiency in Chinese Provinces Based on a Three-Stage SBM-Undesirable Model and an LSTM Model
Authors: Niu Huayong , Zhang Zhishuo , Xiao Yao , Luo Manting , Chen Yumeng
Published: April 2022

As a major carbon-emitting country, there is an urgent need for China to reduce carbon emissions. Studying the carbon emission efficiency of each province helps us to learn about the characteristics and evolution of regional carbon emissions, which is important for proposing effective and targeted measures to achieve the carbon peaking and carbon neutrality goals. This paper measures the carbon emission efficiency of 30 Chinese provinces from 2006 to 2019 based on a three-stage SBM-undesirable model and explores external drivers using stochastic frontier models. The results of the SBM-undesirable model show that the inter-provincial carbon emission efficiency is unevenly distributed and shows a big difference. From the results of the stochastic frontier model analysis, external drivers such as the intensity of finance in environmental protection, the level of economic development, the industrial structure, the level of urbanization, the degree of openness and the level of science as well as technology innovation all have an impact on the emission efficiency. In terms of LSTM model prediction, the model shows an excellent fitting effect, which provides a possible path for carbon emission efficiency prediction. Finally, based on the empirical results and the actual situation of each province in China, this paper proposes relevant feasible suggestions.
What Breeds Conspiracy Theories in COVID-19? The Role of Risk Perception in the Belief in COVID-19 Conspiracy
Authors: Zeng Zhaoxie , Ding Yi , Zhang Yue , Guo Yongyu
Published: April 2022

Conspiracy theories often emerge during public health crises, and can provide some explanation for the causes behind the crises. However, the prevalence of conspiracy theories also poses a serious threat to public health order and hinders the implementation of disease prevention and control measures. No studies have examined the role of multiple risk perceptions in the formation of beliefs in conspiracy theories from a cognitive perspective in the context of the epidemic. In this cross-sectional study, participants filled in an online survey in order to investigate the relationship between epidemic severity and beliefs in conspiracy theories and the mediating role of risk perception in this relationship. The results showed that COVID-19 epidemic severity positively predicted beliefs in both in- and out-group conspiracy theories. Risk perception mediated the positive relationship between COVID-19 epidemic severity and belief in in-group conspiracy theories. These results suggest that in a major public health crisis event: (1) residents at the epicenter may be more prone to believing in both in- and out-group conspiracy theories; and (2) beliefs in in- and out-group conspiracy theories may have different psychological mechanisms. Therefore, conspiracy theories about public health incidents, such as the COVID-19 pandemic, should be classified and treated by policy stakeholders.
Comparison of the Agonist/Antagonist Tensional Balance of the Knee between Two Isokinetic Positions: A Pilot Study on a Sample of High-Level Competitive Soccer Players
Authors: García-Pastor Jorge , Alvear-Ordenes Ildefonso , Arias-Giráldez Diego , Reguera-García María Mercedes , Alonso-Cortés Beatriz
Published: April 2022

Isokinetic knee dynamometry evolves towards more precise techniques, such as the calculation of the functional ratio. This study evaluated the influence of an intermediate hip position called the unified reclined position (URP) compared to the classic sitting position, (CSP) on hamstring eccentric PT values (Hexc30) and conventional (Hcon60/Qcon60) and functional (Hexc30/Qcon60) ratios. Twenty Spanish high-level competitive soccer players (20.4 ± 4.44 years) were evaluated in CSP and in URP. The hip angle in URP (44°) was determined with a passive extensibility test (quadriceps and hamstrings), looking for an agonist/antagonist tension balance. The following were performed: three repetitions (60°/s) and five repetitions (240°/s) in concentric quadriceps and hamstrings mode; and three repetitions (30°/s) in concentric and eccentric for the hamstrings. At 30°/s, the CSP presents higher values of maximal eccentric hamstring strength than URP, (Dom + N-Dom leg (Nm): CSP = 148.3 ± 19.5 vs. URP 143.5 ± 23.2); p = 0.086 (n.s.). The conventional relationship did not show data justifying the preference for URP over CSP (p = 0.86 (n.s.)). However, although the functional index did not show significant values (p = 0.97 (n.s.), it did show a greater number of subjects with imbalances measured in URP (five in URP vs. two in CSP). An assessment angle of the hip closer to sports reality seems to favor the use of the URP as a complementary method to the CSP. These data stimulate new studies using URP together with the classic protocol.
Reactivity of Myoglobin Reconstituted with Cobalt Corrole toward Hydrogen Peroxide
Authors: Oohora Koji , Tomoda Hirotaka , Hayashi Takashi
Published: April 2022

The protein matrix of natural metalloenzymes regulates the reactivity of metal complexes to establish unique catalysts. We describe the incorporation of a cobalt complex of corrole (CoCor), a trianionic porphyrinoid metal ligand, into an apo-form of myoglobin to provide a reconstituted protein (rMb(CoCor)). This protein was characterized by UV-vis, EPR, and mass spectroscopic measurements. The reaction of rMb(CoCor) with hydrogen peroxide promotes an irreversible oxidation of the CoCor cofactor, whereas the same reaction in the presence of a phenol derivative yields the cation radical form of CoCor. Detailed kinetic investigations indicate the formation of a transient hydroperoxo complex of rMb(CoCor) which promotes the oxidation of the phenol derivatives. This mechanism is significantly different for native heme-dependent peroxidases, which generate a metal-oxo species as an active intermediate in a reaction with hydrogen peroxide. The present findings of unique reactivity will contribute to further design of artificial metalloenzymes.
Cytokines from Bench to Bedside: A Retrospective Study Identifies a Definite Panel of Biomarkers to Early Assess the Risk of Negative Outcome in COVID-19 Patients
Authors: Fabris Martina , Del Ben Fabio , Sozio Emanuela , Beltrami Antonio Paolo , Cifù Adriana , Bertolino Giacomo , Caponnetto Federica , Cotrufo Marco , Tascini Carlo , Curcio Francesco
Published: April 2022

The main aim of this study was to identify the most relevant cytokines which, when assessed in the earliest stages from hospital admission, may help to select COVID-19 patients with worse prognosis. A retrospective observational study was conducted in 415 COVID-19 patients (272 males; mean age 68 ± 14 years) hospitalized between May 2020 and March 2021. Within the first 72 h from hospital admission, patients were tested for a large panel of biomarkers, including C-reactive protein (CRP), Mid-regional proadrenomedullin (MR-proADM), Interferon-γ, interleukin 6 (IL-6), IL-1β, IL-8, IL-10, soluble IL2-receptor-α (sIL2Rα), IP10 and TNFα. Extensive statistical analyses were performed (correlations, t-tests, ranking tests and tree modeling). The mortality rate was 65/415 (15.7%) and a negative outcome (death and/or orotracheal intubation) affected 98/415 (23.6%) of cases. Univariate tests showed the majority of biomarkers increased in severe patients, but ranking tests helped to select the best variables to put on decisional tree modeling which identified IL-6 as the first dichotomic marker with a cut-off of 114 pg/mL. Then, a good synergy was found between IL-10, MR-proADM, sIL2Rα, IP10 and CRP in increasing the predictive value in classifying patients at risk or not for a negative outcome. In conclusion, beside IL-6, a panel of other cytokines representing the degree of immunoparalysis and the anti-inflammatory response (IP10, sIL2Rα and IL-10) showed synergic role when combined to biomarkers of systemic inflammation and endothelial dysfunction (CRP, MR-proADM) and may also better explain disease pathogenesis and suggests targeted intervention.
Functionalisation of Inorganic Material Surfaces with Staphylococcus Protein A: A Molecular Dynamics Study
Authors: Farouq Mohammed A. H. , Kubiak-Ossowska Karina , Al Qaraghuli Mohammed M. , Ferro Valerie A. , Mulheran Paul A.
Published: April 2022

Staphylococcus protein A (SpA) is found in the cell wall of Staphylococcus aureus bacteria. Its ability to bind to the constant Fc regions of antibodies means it is useful for antibody extraction, and further integration with inorganic materials can lead to the development of diagnostics and therapeutics. We have investigated the adsorption of SpA on inorganic surface models such as experimentally relevant negatively charged silica, as well as positively charged and neutral surfaces, by use of fully atomistic molecular dynamics simulations. We have found that SpA, which is itself negatively charged at pH7, is able to adsorb on all our surface models. However, adsorption on charged surfaces is more specific in terms of protein orientation compared to a neutral Au (111) surface, while the protein structure is generally well maintained in all cases. The results indicate that SpA adsorption is optimal on the siloxide-rich silica surface, which is negative at pH7 since this keeps the Fc binding regions free to interact with other species in solution. Due to the dominant role of electrostatics, the results are transferable to other inorganic materials and pave the way for new diagnostic and therapeutic designs where SpA might be used to conjugate antibodies to nanoparticles.
In Vitro Cytotoxicity, Colonisation by Fibroblasts and Antimicrobial Properties of Surgical Meshes Coated with Bacterial Cellulose
Authors: Dydak Karolina , Junka Adam , Nowacki Grzegorz , Paleczny Justyna , Szymczyk-Ziółkowska Patrycja , Górzyńska Aleksandra , Aniołek Olga , Bartoszewicz Marzenna
Published: April 2022

Hernia repairs are the most common abdominal wall elective procedures performed by general surgeons. Hernia-related postoperative infective complications occur with 10% frequency. To counteract the risk of infection emergence, the development of effective, biocompatible and antimicrobial mesh adjuvants is required. Therefore, the aim of our in vitro investigation was to evaluate the suitability of bacterial cellulose (BC) polymer coupled with gentamicin (GM) antibiotic as an absorbent layer of surgical mesh. Our research included the assessment of GM-BC-modified meshes’ cytotoxicity against fibroblasts ATCC CCL-1 and a 60-day duration cell colonisation measurement. The obtained results showed no cytotoxic effect of modified meshes. The quantified fibroblast cells levels resembled a bimodal distribution depending on the time of culturing and the type of mesh applied. The measured GM minimal inhibitory concentration was 0.47 µg/mL. Results obtained in the modified disc-diffusion method showed that GM-BC-modified meshes inhibited bacterial growth more effectively than non-coated meshes. The results of our study indicate that BC-modified hernia meshes, fortified with appropriate antimicrobial, may be applied as effective implants in hernia surgery, preventing risk of infection occurrence and providing a high level of biocompatibility with regard to fibroblast cells.
Polymerizable Skin Hydrogel for Full Thickness Wound Healing
Authors: Persinal-Medina Mairobi , Llames Sara , Chacón Manuel , Vázquez Natalia , Pevida Marta , Alcalde Ignacio , Alonso-Alonso Sergio , Martínez-López Laura María , Merayo-Lloves Jesús , Meana Álvaro
Published: April 2022

The skin is the largest organ in the human body, comprising the main barrier against the environment. When the skin loses its integrity, it is critical to replace it to prevent water loss and the proliferation of opportunistic infections. For more than 40 years, tissue-engineered skin grafts have been based on the in vitro culture of keratinocytes over different scaffolds, requiring between 3 to 4 weeks of tissue culture before being used clinically. In this study, we describe the development of a polymerizable skin hydrogel consisting of keratinocytes and fibroblast entrapped within a fibrin scaffold. We histologically characterized the construct and evaluated its use on an in vivo wound healing model of skin damage. Our results indicate that the proposed methodology can be used to effectively regenerate skin wounds, avoiding the secondary in vitro culture steps and thus, shortening the time needed until transplantation in comparison with other bilayer skin models. This is achievable due to the instant polymerization of the keratinocytes and fibroblast combination that allows a direct application on the wound. We suggest that the polymerizable skin hydrogel is an inexpensive, easy and rapid treatment that could be transferred into clinical practice in order to improve the treatment of skin wounds.
Physiological and Environmental Regulation of Seed Germination: From Signaling Events to Molecular Responses
Authors: Baudouin Emmanuel , Puyaubert Juliette , Bailly Christophe
Published: April 2022

A timely and efficient seed germination is critical for plantlets’ establishment and robustness as well as plant development and plant performance in both natural ecosystems and agrosystems [...]
Supplementation with SCFAs Re-Establishes Microbiota Composition and Attenuates Hyperalgesia and Pain in a Mouse Model of NTG-Induced Migraine
Authors: Lanza Marika , Filippone Alessia , Casili Giovanna , Giuffrè Letterio , Scuderi Sarah Adriana , Paterniti Irene , Campolo Michela , Cuzzocrea Salvatore , Esposito Emanuela
Published: April 2022

Migraine is a common brain-disorder that affects 15% of the population. Converging evidence shows that migraine is associated with gastrointestinal disorders. However, the mechanisms underlying the interaction between the gut and brain in patients with migraine are not clear. In this study, we evaluated the role of the short-chain fatty acids (SCFAs) as sodium propionate (SP) and sodium butyrate (SB) on microbiota profile and intestinal permeability in a mouse model of migraine induced by nitroglycerine (NTG). The mice were orally administered SB and SP at the dose of 10, 30 and 100 mg/kg, 5 min after NTG intraperitoneal injections. Behavioral tests were used to evaluate migraine-like pain. Histological and molecular analyses were performed on the intestine. The composition of the intestinal microbiota was extracted from frozen fecal samples and sequenced with an Illumina MiSeq System. Our results demonstrated that the SP and SB treatments attenuated hyperalgesia and pain following NTG injection. Moreover, SP and SB reduced histological damage in the intestine and restored intestinal permeability and the intestinal microbiota profile. These results provide corroborating evidence that SB and SP exert a protective effect on central sensitization induced by NTG through a modulation of intestinal microbiota, suggesting the potential application of SCFAs as novel supportive therapies for intestinal disfunction associated with migraine.
Efficient Large-Scale and Scarless Genome Engineering Enables the Construction and Screening of Bacillus subtilis Biofuel Overproducers
Authors: Tian Jiheng , Xing Baowen , Li Mengyuan , Xu Changgeng , Huo Yi-Xin , Guo Shuyuan
Published: April 2022

Bacillus subtilis is a versatile microbial cell factory that can produce valuable proteins and value-added chemicals. Long fragment editing techniques are of great importance for accelerating bacterial genome engineering to obtain desirable and genetically stable host strains. Herein, we develop an efficient CRISPR-Cas9 method for large-scale and scarless genome engineering in the Bacillus subtilis genome, which can delete up to 134.3 kb DNA fragments, 3.5 times as long as the previous report, with a positivity rate of 100%. The effects of using a heterologous NHEJ system, linear donor DNA, and various donor DNA length on the engineering efficiencies were also investigated. The CRISPR-Cas9 method was then utilized for Bacillus subtilis genome simplification and construction of a series of individual and cumulative deletion mutants, which are further screened for overproducer of isobutanol, a new generation biofuel. These results suggest that the method is a powerful genome engineering tool for constructing and screening engineered host strains with enhanced capabilities, highlighting the potential for synthetic biology and metabolic engineering.