bluuebunny/crossref_metadata_2025 huggingface dataset:
Dexmedetomidine Preserves Activity of Neurons in Primary Somatosensory Cortex Compared to Propofol and Ketamine
Authors: Xia Mu-Chao , Guo Juan , Ding Yan , Shi Zi-Qi , Du Fang , Wang Kai , Miao Chang-Hong , Liang Chao
Published: December 2022

General anesthesia has been shown to induce significant changes in the functional connectivity of the cerebral cortex. However, traditional methods such as electroencephalography (EEG) or functional magnetic resonance imaging (fMRI) lack the spatial resolution to study the effects of general anesthesia on individual cortical neurons. This study aimed to use high-resolution two-photon imaging, which can provide single-neuron resolution, to investigate the characteristics of consciousness under general anesthesia. We used C57BL/6J and Thy1-GCamp6s mice and found that at similar levels of sedation, as measured by EEG, dexmedetomidine did not significantly inhibit the spontaneous activity of neuronal somata in the S1 cortex, but preserved the frequency of calcium events in neuronal spines. In contrast, propofol and ketamine dramatically inhibited the spontaneous activity of both neuronal somata and spines. The S1 cortex still responded to whisker stimulation under dexmedetomidine anesthesia, but not under propofol or ketamine anesthesia. Our results suggest that dexmedetomidine anesthesia has unique neuronal properties associated with its ability to facilitate easy awakening in the clinic. These findings provide insights into the development of more effective strategies for monitoring consciousness during general anesthesia.
Workplace Incivility and Employee Performance: Does Trust in Supervisors Matter? (A Dual Theory Perspective)
Authors: Saleem Farida , Malik Muhammad Imran , Asif Iqra , Qasim Awais
Published: December 2022

Employee performance is the backbone of achieving competitiveness and sustainability. This study aims to examine the impact of workplace incivility on employee performance. In addition, trust in supervisors is examined as a mediator. The conservation of resources (COR) theory and Maslow’s hierarchy of needs theory provided the grounds for developing the framework. The data were collected through closed-ended questionnaires and were analyzed using structural equation modeling with SmartPLS. The results affirmed that incivility is harmful to the performance of employees, and that trust in supervisors helps employees to perform well. The trust in the supervisor significantly mediates the incivility–performance relationship. The examination of the proposed model through the lens of two theories as well as the study of low-intensity deviant workplace behavior in a collectivist and developing economy are the contributions of this study to the growing body of literature. However, the use of a single sector was one of the limitations of this study.
The Mediating Role of Spiritual Intelligence on Well-Being and Life Satisfaction among Nurses in the Context of the COVID-19 Pandemic: A Path Analysis
Authors: Alrashidi Nojoud , Alreshidi Maha Sanat , Dator Wireen Leila , Maestrado Richard , Villareal Sandro , Buta Joyce , Pangket Petelyne , Mostoles Romeo Jr , Gonzales Analita , Mina Enrique , Pasay An Eddieson
Published: December 2022

Introduction: As they are satisfied with life, nurses who demonstrate spiritual intelligence and well-being at work are tremendous assets to an organisation. This study aimed to determine the mediating effect of spiritual intelligence on the well-being and life satisfaction of nurses in the context of COVID-19. Methods: This research employed a cross-sectional study design. It was conducted in the Hail region of Saudi Arabia. The participants were government hospital nurses who were chosen using a multi-stage sampling method. A total of 1121 (75% response rate) nurses participated in the study. Data gathering was conducted from July to September 2022. Results: The life satisfaction level of the participants was deemed positive. Their well-being was better (10/15), and they rated high in spiritual intelligence (90/120). Life satisfaction had a strong association with well-being (r = 0.640, p < 0.001), but a weak association with spiritual intelligence (r = 0.391, p < 0.001). In comparison, well-being had a moderate association with spiritual intelligence (r = 0.551, p < 0.001). The direct effect of well-being on spiritual intelligence was positive and significant (β = 0.7817, p < 0.0001), and that of spiritual intelligence on life satisfaction was positive and significant (β = 0.1082, p = 0.0257). The direct effect of well-being on life satisfaction was also positive and significant (β = 1.5985, p < 0.0001). Conversely, well-being had an indirect impact on life satisfaction (β = 0.0846), and this effect was significant. Overall, the impact of well-being on life satisfaction was positive and significant (β = 1.6831, p < 0.0001). Conclusion: The nurses in this study were deemed satisfied, to have better well-being, and better spiritual intelligence. Life satisfaction has a strong association with well-being but a weak one with spiritual intelligence, while well-being has a moderate association with spiritual intelligence. Overall, spiritual intelligence was found to have a mediating effect on the relationship between well-being and life satisfaction. These findings suggest that an increase in spiritual growth can serve as the foundation for people to live better and more integrated lives.
Pull-Out of Pristine and Functionalized Carbon Nanotubes from Cement: A Molecular Modelling Study
Authors: Lado-Touriño Isabel
Published: December 2022

Carbon nanotubes (CNTs) are widely used as reinforcements in cement-based composites. The improvement in the mechanical properties of the resulting materials depends on the characteristics of the interface formed between CNTs and the cement matrix. The experimental characterization of the interfacial properties of these composites is still limited and hard to achieve with currently available technologies. In this work, molecular dynamics and molecular mechanics pull-out simulations of pristine and functionalized CNTs, taken from a tobermorite crystal, were carried out to study interfacial shear strength (ISS) from an atomic perspective. ISS was calculated from the potential energy of the systems. The effects of the CNT diameter and the degree of functionalization on the pull-out process were analyzed according to the ISS and non-bonded energy results. The influence of H-bonding and electrostatic interactions between the CNT and the matrix were also studied. The results show that ISS decreases with increasing CNT radius for pristine CNTs and depends upon the number of H-bonds for functionalized CNTs. ISS values are positively correlated to Enon-bonded energy, which is related to the number of carboxyl groups on the CNT surface. A high degree of functionalization increases both the number of H-bonds and the number of Ca2+-O interactions between the CNT and the tobermorite surface. This results in a stronger interfacial interaction and, therefore, an elevated ISS value.
Tumoricidal, Temozolomide- and Radiation-Sensitizing Effects of KCa3.1 K+ Channel Targeting In Vitro Are Dependent on Glioma Cell Line and Stem Cell Fraction
Authors: Stransky Nicolai , Ganser Katrin , Naumann Ulrike , Huber Stephan M. , Ruth Peter
Published: December 2022

Reportedly, the intermediate-conductance Ca2+-activated potassium channel KCa3.1 contributes to the invasion of glioma cells into healthy brain tissue and resistance to temozolomide and ionizing radiation. Therefore, KCa3.1 has been proposed as a potential target in glioma therapy. The aim of the present study was to assess the variability of the temozolomide- and radiation-sensitizing effects conferred by the KCa3.1 blocking agent TRAM-34 between five different glioma cell lines grown as differentiated bulk tumor cells or under glioma stem cell-enriching conditions. As a result, cultures grown under stem cell-enriching conditions exhibited indeed higher abundances of mRNAs encoding for stem cell markers compared to differentiated bulk tumor cultures. In addition, stem cell enrichment was paralleled by an increased resistance to ionizing radiation in three out of the five glioma cell lines tested. Finally, TRAM-34 led to inconsistent results regarding its tumoricidal but also temozolomide- and radiation-sensitizing effects, which were dependent on both cell line and culture condition. In conclusion, these findings underscore the importance of testing new drug interventions in multiple cell lines and different culture conditions to partially mimic the in vivo inter- and intra-tumor heterogeneity.
Natural Products/Bioactive Compounds as a Source of Anticancer Drugs
Authors: Asma Syeda Tasmia , Acaroz Ulas , Imre Kálmán , Morar Adriana , Shah Syed Rizwan Ali , Hussain Syed Zajif , Arslan-Acaroz Damla , Demirbas Hayri , Hajrulai-Musliu Zehra , Istanbullugil Fatih Ramazan , Soleimanzadeh Ali , Morozov Dmitry , Zhu Kui , Herman Viorel , Ayad Abdelhanine , Athanassiou Christos , Ince Sinan
Published: December 2022

Cancer is one of the major deadly diseases globally. The alarming rise in the mortality rate due to this disease attracks attention towards discovering potent anticancer agents to overcome its mortality rate. The discovery of novel and effective anticancer agents from natural sources has been the main point of interest in pharmaceutical research because of attractive natural therapeutic agents with an immense chemical diversity in species of animals, plants, and microorganisms. More than 60% of contemporary anticancer drugs, in one form or another, have originated from natural sources. Plants and microbial species are chosen based on their composition, ecology, phytochemical, and ethnopharmacological properties. Plants and their derivatives have played a significant role in producing effective anticancer agents. Some plant derivatives include vincristine, vinblastine, irinotecan, topotecan, etoposide, podophyllotoxin, and paclitaxel. Based on their particular activity, a number of other plant-derived bioactive compounds are in the clinical development phase against cancer, such as gimatecan, elomotecan, etc. Additionally, the conjugation of natural compounds with anti-cancerous drugs, or some polymeric carriers particularly targeted to epitopes on the site of interest to tumors, can generate effective targeted treatment therapies. Cognizance from such pharmaceutical research studies would yield alternative drug development strategies through natural sources which could be economical, more reliable, and safe to use.
Coxsackievirus Group B3 Has Oncolytic Activity against Colon Cancer through Gasdermin E-Mediated Pyroptosis
Authors: Zhang Yejia , Xu Tian , Tian Huizhen , Wu Jianfeng , Yu Xiaomin , Zeng Lingbing , Liu Fadi , Liu Qiong , Huang Xiaotian
Published: December 2022

Colon cancer is the second leading cause of cancer-related death, and there are few effective therapies for colon cancer. This study explored the use of coxsackievirus group B3 (CVB3) as an oncolytic virus for the treatment of colon cancer. In this study, we verified that CVB3 induces death of colon cancer cell lines by directly observing cell morphology and Western blot results, and observed the oncolytic effects of CVB3 by constructing an immunodeficient nude mice model. Our data show that CVB3 induces pyroptosis in colon cancer cell lines. Mechanistically, we demonstrated that CVB3 causes cleavage of gasdermin E (GSDME), but not gasdermin D (GSDMD), by activating caspase-3. This leads to production of GSDME N-termini and the development of pores in the plasma membrane, inducing pyroptosis of colon cancer cell lines. We also demonstrate that CVB3-induced pyroptosis is promoted by reactive oxygen species (ROS). Finally, in vivo studies using immunodeficient nude mice revealed that intratumoral injection of CVB3 led to significant tumor regression. Our findings indicate that CVB3 has oncolytic activity in colon cancer cell lines via GSDME-mediated pyroptosis.
Histone Citrullination Mediates a Protective Role in Endothelium and Modulates Inflammation
Authors: Osca-Verdegal Rebeca , Beltrán-García Jesús , Paes Ana B. , Nacher-Sendra Elena , Novella Susana , Hermenegildo Carlos , Carbonell Nieves , García-Giménez José Luis , Pallardó Federico V.
Published: December 2022

NETosis is a key host immune process against a pathogenic infection during innate immune activation, consisting of a neutrophil “explosion” and, consequently, NET formation, containing mainly DNA, histones, and other nuclear proteins. During sepsis, an exacerbated immune host response to an infection occurs, activating the innate immunity and NETosis events, which requires histone H3 citrullination. Our group compared the circulating histone levels with those citrullinated H3 levels in plasma samples of septic patients. In addition, we demonstrated that citrullinated histones were less cytotoxic for endothelial cells than histones without this post-translational modification. Citrullinated histones did not affect cell viability and did not activate oxidative stress. Nevertheless, citrullinated histones induced an inflammatory response, as well as regulatory endothelial mechanisms. Furthermore, septic patients showed elevated levels of circulating citrullinated histone H3, indicating that the histone citrullination is produced during the first stages of sepsis, probably due to the NETosis process.
Anti-Inflammatory Neutrophil Functions in the Resolution of Inflammation and Tissue Repair
Authors: Loh Waywen , Vermeren Sonja
Published: December 2022

Neutrophils are highly abundant circulating leukocytes that are amongst the first cells to be recruited to sites of infection or sterile injury. Their ability to generate and release powerful cytotoxic products ties with their role in host defence from bacterial and fungal infections. Neutrophilic inflammation is tightly regulated to limit the amount of ‘bystander injury’ caused. Neutrophils were in the past regarded as short-lived, indiscriminate killers of invading microorganisms. However, this view has changed quite dramatically in recent years. Amongst other insights, neutrophils are now recognised to also have important anti-inflammatory functions that are critical for the resolution of inflammation and return to homeostasis. This minireview focusses on anti-inflammatory neutrophil functions, placing a particular focus on recent findings linked to neutrophil cell death, several types of which may be anti-inflammatory (apoptosis, secondary necrosis, and neutrophil extracellular traps). These are discussed together with features that may further promote the clearance of dead cells by efferocytosis and reprogramming of macrophages to promote resolution and repair.
A Combined Risk Score Model to Assess Prognostic Value in Patients with Soft Tissue Sarcomas
Authors: Li Zihua , Duan Zhengwei , Jia Keyao , Yao Yiwen , Liu Kaiyuan , Qiao Yue , Gao Qiuming , Yang Yunfeng , Li Guodong , Shang Anquan
Published: December 2022

A study by Tsvetkov et al. recently published a proposed novel form of copper-induced cell death in Science; however, few studies have looked into the possible mechanism in soft tissue sarcoma (STS). Herein, this study sought to investigate the function of cuproptosis-related genes (CRGs) in the development of tumor-associated immune cells and the prognosis of sarcoma. Herein, this study aimed to explore the role of cuproptosis-related genes (CRGs) in the development, tumor-associated immune cells, and the prognosis of sarcoma. Methods: The prognostic model was established via the least absolute shrinkage and selection operator (LASSO) algorithm as well as multivariate Cox regression analysis. The stromal scores, immune scores, ESTIMA scores, and tumor purity of sarcoma patients were evaluated by the ESTIMATE algorithm. Functional analyses were performed to investigate the underlying mechanisms of immune cell infiltration and the prognosis of CRGs in sarcoma. Results: Two molecular subgroups with different CRG expression patterns were recognized, which showed that patients with a higher immune score and more active immune status were prone to have better prognostic survival. Moreover, GO and KEGG analyses showed that these differentially expressed CRGs were mainly enriched in metabolic/ions-related signaling pathways, indicating that CRGs may have impacts on the immune cell infiltration and prognosis of sarcoma via regulating the bioprocess of mitochondria and consequently affecting the immune microenvironment. The expression levels of CRGs were closely correlated to the immunity condition and prognostic survival of sarcoma patients. Conclusions: The interaction between cuproptosis and immunity in sarcoma may provide a novel insight into the study of molecular mechanisms and candidate biomarkers for the prognosis, resulting in effective treatments for sarcoma patients.
Evaluation of Hormonal Factors in Acne Vulgaris and the Course of Acne Vulgaris Treatment with Contraceptive-Based Therapies in Young Adult Women
Authors: Borzyszkowska Dominika , Niedzielska Mirela , Kozłowski Mateusz , Brodowska Agnieszka , Przepiera Adam , Malczyk-Matysiak Kinga , Cymbaluk-Płoska Aneta , Sowińska-Przepiera Elżbieta
Published: December 2022

Acne vulgaris is a common chronic inflammatory skin disease, which is considered one of the diseases of civilization due to the significant influence of environmental factors on the severity and frequency of these lesions. The aim of this study was to evaluate the hormonal profile of patients before treatment and to assess selected hormonal parameters after treatment. Our first objective was to examine the correlation between the selected hormonal parameters and the severity of acne before treatment. Our second objective was to evaluate the impact of treatment with three therapies, as measured by the selected hormonal parameters and acne severity. Statistical calculations were performed using the R v.4.1.1 statistical calculation environment (IDE RStudio v. 1.4.1717) with a significance level for the statistical tests set at α = 0.05. The results showed that the women in the pre-treatment (T1) and control (C) groups had significant differences in testosterone, androstendione, FAI, SHBG, prolactin, ACTH, and cortisol concentrations. After treatment, there were still significant differences in testosterone, androstendione, FAI, and SHBG concentrations between the post-treatment (T2) and control groups. We concluded that testosterone, androstendione, and cortisol concentrations correlate with acne severity. Acne in adult women may be an important clinical marker of androgen excess syndrome and cannot be considered a transient symptom of puberty. The mainstay of acne treatment is contraceptive therapy (ethonylestradiol and drospirenone). In this study, we confirmed the effectiveness of three contraceptive-based treatments using hormonal parameters and acne severity.
Hepatic Peroxisome Proliferator-Activated Receptor Alpha Dysfunction in Porcine Septic Shock
Authors: Vandewalle Jolien , Garcia Bruno , Timmermans Steven , Vanderhaeghen Tineke , Van Wyngene Lise , Eggermont Melanie , Dufoor Hester , Van Dender Céline , Halimi Fëllanza , Croubels Siska , Herpain Antoine , Libert Claude
Published: December 2022

Despite decades of research, sepsis remains one of the most urgent unmet medical needs. Mechanistic investigations into sepsis have mainly focused on targeting inflammatory pathways; however, recent data indicate that sepsis should also be seen as a metabolic disease. Targeting metabolic dysregulations that take place in sepsis might uncover novel therapeutic opportunities. The role of peroxisome proliferator-activated receptor alpha (PPARɑ) in liver dysfunction during sepsis has recently been described, and restoring PPARɑ signaling has proven to be successful in mouse polymicrobial sepsis. To confirm that such therapy might be translated to septic patients, we analyzed metabolic perturbations in the liver of a porcine fecal peritonitis model. Resuscitation with fluids, vasopressor, antimicrobial therapy and abdominal lavage were applied to the pigs in order to mimic human clinical care. By using RNA-seq, we detected downregulated PPARɑ signaling in the livers of septic pigs and that reduced PPARɑ levels correlated well with disease severity. As PPARɑ regulates the expression of many genes involved in fatty acid oxidation, the reduced expression of these target genes, concomitant with increased free fatty acids in plasma and ectopic lipid deposition in the liver, was observed. The results obtained with pigs are in agreement with earlier observations seen in mice and support the potential of targeting defective PPARɑ signaling in clinical research.
Mitochondrial Autophagy in Ischemic Aged Livers
Authors: Kim Jae-Sung , Chapman William C. , Lin Yiing
Published: December 2022

Mitochondrial autophagy (mitophagy) is a central catabolic event for mitochondrial quality control. Defective or insufficient mitophagy, thus, can result in mitochondrial dysfunction, and ultimately cell death. There is a strong causal relationship between ischemia/reperfusion (I/R) injury and mitochondrial dysfunction following liver resection and transplantation. Compared to young patients, elderly patients poorly tolerate I/R injury. Accumulation of abnormal mitochondria after I/R is more prominent in aged livers than in young counterparts. This review highlights how altered autophagy is mechanistically involved in age-dependent hypersensitivity to reperfusion injury.
Tackling AMR: A Call for a(n Even) More Integrated and Transdisciplinary Approach between Planetary Health and Earth Scientists
Authors: Cole Jennifer , Eskdale Adam , Paul Jonathan D.
Published: December 2022

Antibiotic resistance is a pressing global, one health and planetary health challenge. Links between climate change, antibiotic use, and the emergence of antibiotic resistance have been well documented, but less attention has been given to the impact(s) of earth systems on specific bacterial livestock diseases at a more granular level. Understanding the precise impacts of climate change on livestock health—and in turn the use of antibiotics to address that ill-health—is important in providing an evidence base from which to tackle such impacts and to develop practical, implementable, and locally acceptable solutions within and beyond current antibiotic stewardship programs. In this paper, we set out the case for better integration of earth scientists and their specific disciplinary skill set (specifically, problem-solving with incomplete/fragmentary data; the ability to work across four dimensions and at the interface between the present and deep/geological time) into planetary health research. Then, using a case study from our own research, we discuss a methodology that makes use of risk mapping, a common methodology in earth science but less frequently used in health science, to map disease risk against changing climatic conditions at a granular level. The aim of this exercise is to argue that, by enabling livestock farmers, veterinarians, and animal health observatories to better predict future disease risk and risk impacts based on predicted future climate conditions, earth science can help to provide an evidence base from which to influence policy and develop mitigations. Our example—of climate conditions’ impact on livestock health in Karnataka, India—clearly evidences the benefit of integrating earth scientists into planetary health research.
From Chilblains (Pernio) to Coeliac Disease—Should We Still Consider It Random?
Authors: Mašić Mario , Močić Pavić Ana , Gagro Alenka , Balažin Vučetić Ana , Ožanić Bulić Suzana , Mišak Zrinjka
Published: December 2022

Coeliac disease (CD) is a gluten-triggered, immune-mediated inflammatory disease occurring in genetically predisposed individuals, causing a variety of gastrointestinal and extraintestinal symptoms. The most common cutaneous association of CD is dermatitis herpetiformis, although recent reports have sought to link CD with other dermatological and autoimmune diseases. Chilblain, also called pernio, is usually a benign, superficial and localized inflammatory skin disorder that results from a maladaptive vascular response to non-freezing cold. We present a patient with pernio (chilblains) and newly diagnosed CD, with a significant intestinal lesion–total villous atrophy, as there are only two known cases of this feature associated with CD published in the literature. In the workup of chilblains (pernio) in children, an active case finding for coeliac disease should be conducted with coeliac-specific serology testing.
Application and Molecular Mechanisms of Extracellular Vesicles Derived from Mesenchymal Stem Cells in Osteoporosis
Authors: Yang Yajing , Yuan Lei , Cao Hong , Guo Jianmin , Zhou Xuchang , Zeng Zhipeng
Published: December 2022

Osteoporosis (OP) is a chronic bone disease characterized by decreased bone mass, destroyed bone microstructure, and increased bone fragility. Accumulative evidence shows that extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) (MSC-EVs), especially exosomes (Exos), exhibit great potential in the treatment of OP. However, the research on MSC-EVs in the treatment of OP is still in the initial stage. The potential mechanism has not been fully clarified. Therefore, by reviewing the relevant literature of MSC-EVs and OP in recent years, we summarized the latest application of bone targeted MSC-EVs in the treatment of OP and further elaborated the potential mechanism of MSC-EVs in regulating bone formation, bone resorption, bone angiogenesis, and immune regulation through internal bioactive molecules to alleviate OP, providing a theoretical basis for the related research of MSC-EVs in the treatment of OP.
Sustainable Coastal Business Strategies for Cultured Pearl Sectors: Agenda Development for Coast-Area Actors’ Collaboration
Authors: Oe Hiroko , Yamaoka Yasuyuki
Published: December 2022

This study was conducted to propose and identify suggestions for pathways to maintain the sustainability of the pearl industry, a cultural value asset rooted in the region, and to revitalize coastal communities through the pearl industry. Using a qualitative approach, this study sought the opinions of relevant stakeholders based on key themes from the literature review and compiled an agenda for further research and discussion. Specifically, focus group discussions were held with pearl industry stakeholders, local residents, the pearl industry and organizations, tourists, and the public sector, and the views among the four stakeholder groups were analyzed. As a result, the following factors were identified as likely to contribute to the sustainability of the cultured pearl industry: (1) co-creation of value through collaboration between the local stakeholders and local communities (satoumi), (2) efforts to pass on and innovate traditional cultured pearl technology seeking global markets, and (3) enhancement of industrial support measures in coastal areas through collaboration between residents, industry, and government to create an ecology-focused inbound tourism strategy. Stakeholders are strongly aware of the need to promote to the world the value of unique cultured pearls and gemstones that rely on traditional Japanese technology and to attract tourists, along with the development of the next generation of pearl industry leaders, but further systematic intervention is required to achieve this goal. The results of this study are expected to serve as a basis for the next steps in presenting further suggestions through integrated analysis with quantitative economic data. They may also provide guidance for the development of pathways to regional development through the revitalization of local industries and tourism innovation in other coastal regions of the world.
On Variable Scale Evolution of Stress and Strain of TA2 Titanium Plate in Combined Hammering
Authors: Xiao Xudong , Zhang Bolun , Qiao Dan , Li Yong , Zhao Renfeng , Zhao Pengkang
Published: December 2022

Combined peening composed of multiple peening processes or peening media is a surface treatment method for comprehensive control of the macro shape and performance of the part. Compared to combined peening, the impact kinetic energy of the combined hammering can be easier to control over a wide range, and the hammer tool head size is larger than the shot. This paper focused on investigating the effect of combined hammering treatment, 6 mm and 14 mm tool heads with peening density 3.7 to 4.2/mm2, on the variable scale evolution of titanium TA2. Three types of contact relation between the tool head and existing dimple were proposed for impacting at the same position. The size of the dimple of combined hammering varies in width or depth direction, resulting in nest morphology composed of different size dimples. The cross-section microstructure of the test plate was observed, and the gradient changes of dislocation, slip, and grain size are smoothed by combined hammering. The change in hammer tool head size makes the target plastic deform at different depths. The hammering sequence has a significant influence on the evolution of stress and strain fields. When the tool head is first large and then small, a large compressive residual stress near the surface is introduced, about 1000 Mpa; on the contrary, the compressive residual stress distributes uniformly in the depth direction, with an affected layer depth of about 4.4 mm. The measured dimple size and residual stress verified the reliability of the simulation results.
Effect of Enzymatic Hydrolysis on Solubility and Emulsifying Properties of Lupin Proteins (Lupinus luteus)
Authors: Opazo-Navarrete Mauricio , Burgos-Díaz César , Garrido-Miranda Karla A. , Acuña-Nelson Sergio
Published: December 2022

Solubility and emulsifying properties are important functional properties associated with proteins. However, many plant proteins have lower techno-functional properties, which limit their functional performance in many formulations. Therefore, the objective of this study was to investigate the effect of protein hydrolysis by commercial enzymes to improve their solubility and emulsifying properties. Lupin protein isolate (LPI) was hydrolyzed by 7 commercial proteases using different E/S ratios and hydrolysis times while the solubility and emulsifying properties were evaluated. The results showed that neutral and alkaline proteases are most efficient in hydrolyzing lupin proteins than acidic proteases. Among the proteases, Protamex® (alkaline protease) showed the highest DH values after 5 h of protein hydrolysis. Meanwhile, protein solubility of LPI hydrolysates was significantly higher (p < 0.05) than untreated LPI at all pH analyzed values. Moreover, the emulsifying capacity (EC) of undigested LPI was lower than most of the hydrolysates, except for acidic proteases, while emulsifying stability (ES) was significantly higher (p < 0.05) than most LPI hydrolysates by acidic proteases, except for LPI hydrolyzed with Acid Stable Protease with an E/S ratio of 0.04. In conclusion, the solubility, and emulsifying properties of lupin (Lupinus luteus) proteins can be improved by enzymatic hydrolysis using commercial enzymes.
Comparison of Dynamical and Empirical Simulation Methods of Secondary Dendrite Arm Coarsening
Authors: Roósz András , Rónaföldi Arnold , Svéda Mária , Veres Zsolt
Published: December 2022

The physical and mechanical properties of an entirely (wrought alloys) or partly (cast alloys) dendritically solidified alloy strongly depend on the secondary dendrite arm spacing (SDAS). The casting practice and the simulation of solidification need a usable but simple method to calculate the SDAS during and at the end of solidification as a function of the cooling rate. Based on many solidification experiments, a simple equation to calculate the SDAS (empirical method) is known to use the local solidification time, which can be obtained from the measured cooling curves (equiaxed solidification), or can be calculated from the temperature gradient and front velocity (directional solidification). This equation is not usable for calculating the SDAS during solidification. Kirkwood developed a semi-empirical method based on the liquid phase’s diffusion, which contains only one geometric factor that seems constant for different alloys. This equation contains some physical parameters that depend on the temperature, so the equation cannot be integral in closed form. In the present work, first, we show the effect of the curvature of the solid/liquid interface on the equilibrium concentrations and then the different processes of SDA coarsening. In our earlier paper, we demonstrated that using the empirical method, the final SDAS can be calculated with acceptable correctness in the case of four unidirectional solidification experiments of Al-7wt%Si alloy. The present work shows that numerically integrated Kirkwood’s equations used the known cooling curve; the SDAS can be calculated at the end and during solidification in good agreement with these experimental results. Compared to the two calculation methods, we stated that the correctness of the methods is similar. Still, the results of the solidification simulation (the microsegregation) will be more correct using the dynamical method. It is also shown that with the dynamical method, the SDAS can be calculated from any type of cooling curve, and using the dynamical method, it is proved that some different SDASs could belong to the same local solidification time.
Hyperbolic Behavior and Antiferromagnetic Order in Rare-Earth Tellurides
Authors: Gjerde Jonathan , Jishi Radi A.
Published: December 2022

Quasi-2D materials have received much attention in recent years for their unusual physical properties. Among the most investigated of these materials are the rare-earth tellurides, which are primarily studied because they exhibit charge density waves and other quantum phenomena and have a high degree of tunability. In this paper, we examine the optical and magnetic properties of several rare-earth tellurides and find that they are antiferromagnetic materials with hyperbolic dispersion. Hyperbolic materials have very promising applications in sub-diffraction-limit optics, nanolithography, and spontaneous emission engineering, but these applications are hampered by low-quality hyperbolic materials. Rare-earth tellurides may provide insight into solving these issues if their properties can be properly tuned using the large variety of techniques already explored in the literature.
Zinc Finger Proteins in Head and Neck Squamous Cell Carcinomas: ZNF540 May Serve as a Biomarker
Authors: Sobocińska Joanna , Nowakowska Joanna , Molenda Sara , Olechnowicz Anna , Guglas Kacper , Kozłowska-Masłoń Joanna , Kazimierczak Urszula , Machnik Marta , Oleksiewicz Urszula , Teresiak Anna , Lamperska Katarzyna , Kolenda Tomasz
Published: December 2022

Head and neck squamous cell carcinoma (HNSCC) is one of the ten most common cancers. Most cancer cases originate from alcohol and tobacco consumption. However, studies have demonstrated that human papillomavirus (HPV) infection, particularly HPV-16, may also significantly influence disease progression. The KRAB-ZNF family of genes is involved in epigenetic suppression, and its involvement in carcinogenesis is the subject of extensive studies. The available literature data demonstrate that they may play different roles, both as tumor suppressors and oncogenes. In this study, six ZNF genes, ZFP28, ZNF132, ZNF418, ZNF426, ZNF540, and ZNF880, were tested using several in silico approaches based on the TCGA and GEO datasets. Our analyses indicate that the expression of the analyzed ZNFs was significantly downregulated in tumor tissues and depended on tumor localization. The expression levels of ZNFs differed between HPV-positive vs. HPV-negative patients depending on the clinical-pathological parameters. More specifically, the patients with higher levels of ZNF418 and ZNF540 showed better survival rates than those with a lower expression. In addition, the level of ZNF540 expression in HPV-positive (HPV(+)) patients was higher than in HPV-negative (HPV(−)) patients (p < 0.0001) and was associated with better overall survival (OS). In conclusion, we demonstrate that ZNF540 expression highly correlates with HPV infection, which renders ZNF540 a potential biomarker for HNSCC prognosis and treatment.
Reliability Analysis of Steel Bridge Girders Strengthened with CFRP Considering the Debonding of Adhesive Layer
Authors: Vo Duy Hung , Do Viet Hai , Tran Quang Vy , Nguyen Minh Hai , Hoang Trong Lam
Published: December 2022

One issue to consider while designing and constructing steel girders reinforced with carbon fiber-reinforced polymer (CFRP) plates in bridges is debonding failure. Previous studies showed that the parameters such as characteristics of material, load, adhesive, and CFRP plates have an effect on the failure probability of steel girder, which is represented by the reliability index. Therefore, this study analyzes the reliability indices of steel girders in bridges strengthened with CFRP plates to clarify the effects of debonding failure. Debonding and strength limit states are used to compare differences in reliability indices of different design scenarios. Strength and debonding margin the functions for the strength limit state and debonding limit state will be established in this study. The probability of failure is determined by a Monte Carlo simulation (MCS). It is found that the reliability index of debonding limit state is much lower than that of the strength limit state. This shows that the debonding failure should be considered significant in the reliability analysis of steel bridge girders strengthened with CFRP plate.
RF Source Localization Using Multiple UAVs through a Novel Geometrical RSSI Approach
Authors: Güzey Nurbanu
Published: December 2022

In this paper, a novel geometrical localization scheme based on the Received Signal Strength Indicator (RSSI) is developed for a group of unmanned aerial vehicles (UAVs). Since RSSI-based localization does not require complicated hardware, it is the correct choice for RF target localization. In this promising work, unlike the other techniques given in the literature, transmit power or path loss exponent information is not needed. The procedure depends on the received power difference of each receiver in UAVs. In the developed scheme, four UAVs forming two groups fly in perpendicular planes. Each UAV in the group moves in a circle, keeping its distance from the plane’s center until it gets equal power with the other members of its group. Using this movement rate, lines passing through the source position are calculated. The intersection of these lines gives the position of the RF target. However, in a noisy environment, the lines do not intersect at one point. Therefore, the algorithm given in the manuscript finds a point that has a minimum distance to all lines and is also developed. Simulation results are provided at the end of the manuscript to verify our theoretical claims.
Study of Urban Logistics Drone Path Planning Model Incorporating Service Benefit and Risk Cost
Authors: Shao Quan , Li Jiaming , Li Ruoheng , Zhang Jiangao , Gao Xiaobo
Published: December 2022

The application of drones provides a powerful solution for “the last-mile” logistics services, while the large-scale implementation of logistics drone services will threaten the safety of buildings, pedestrians, vehicles, and other elements in the urban environment. The balance of risk cost and service benefit is accordingly crucial to managing logistics drones. In this study, we proposed a cost-benefit assessment model for quantifying risk cost and service benefit in the urban environment. In addition, a global heuristic path search rule was developed to solve the path planning problem based on risk mitigation and customer service. The cost-benefit assessment model quantifies the risk cost from three environmental elements (buildings, pedestrians, and vehicles) threatened by drone operations based on the collision probability, and the service benefit based on the characteristics of logistics service customers. To explore the effectiveness of the model in this paper, we simulate and analyse the effects of different risk combinations, unknown risk zones, and risk-benefit preferences on the path planning results. The results show that compared with the traditional shortest-distance method, the drone path planning method proposed in this paper can accurately capture the distribution of risks and customers in the urban environment. It is highly reusable in ensuring service benefits while reducing risk costs and generating a cost-effective path for logistics drones. We also compare the algorithm in this paper with the A* algorithm and verify that our algorithm improves the solution quality in complex environments.
Asymmetric Interactions Induce Bistability and Switching Behavior in Models of Collective Motion
Authors: Strömbom Daniel , Tulevech Grace , Giunta Rachel , Cullen Zachary
Published: December 2022

Moving animal groups often spontaneously change their group structure and dynamics, but standard models used to explain collective motion in animal groups are typically unable to generate changes of this type. Recently, a model based on attraction, repulsion and asymmetric interactions designed for specific fish experiments was shown capable of producing such changes. However, the origin of the model’s ability to generate them, and the range of this capacity, remains unknown. Here we modify and extend this model to address these questions. We establish that its ability to generate groups exhibiting changes depends on the size of the blind zone parameter β. Specifically, we show that for small β swarms and mills are generated, for larger β polarized groups forms, and for a region of intermediate β values there is a bistability region where continuous switching between milling and polarized groups occurs. We also show that the location of the bistability region depends on group size and the relative strength of velocity alignment when this interaction is added to the model. These findings may contribute to advance the use of self-propelled particle models to explain a range of disruptive phenomena previously thought to be beyond the capabilities of such models.
Existence of Classical Solutions for Nonlinear Elliptic Equations with Gradient Terms
Authors: Li Yongxiang , Ma Weifeng
Published: December 2022

This paper deals with the existence of solutions of the elliptic equation with nonlinear gradient term −Δu=f(x,u,∇u) on Ω restricted by the boundary condition u|∂Ω=0, where Ω is a bounded domain in RN with sufficiently smooth boundary ∂Ω, N≥2, and f:Ω¯×R×RN→R is continuous. The existence results of classical solutions and positive solutions are obtained under some inequality conditions on the nonlinearity f(x,ξ,η) when |(ξ,η)| is small or large enough.
4E Assessment of an Organic Rankine Cycle (ORC) Activated with Waste Heat of a Flash–Binary Geothermal Power Plant
Authors: Ambriz-Díaz Víctor M. , Rosas Israel Y. , Chávez Oscar , Rubio-Maya Carlos
Published: December 2022

In this paper, the 4E assessment (Energetic, Exergetic, Exergoeconomic and Exergoenvironmental) of a low-temperature ORC activated by two different alternatives is presented. The first alternative (S1) contemplates the activation of the ORC through the recovery of waste heat from a flash–binary geothermal power plant. The second alternative (S2) contemplates the activation of the ORC using direct heat from a geothermal well. For both alternatives, the energetic and exergetic models were established. At the same time, the economic and environmental impact models were developed. Finally, based on the combination of the exergy concepts and the economic and ecological indicators, the exergoeconomic and exergoenvironmental performances of the ORC were obtained. The results show higher economic, exergoeconomic and exergoenvironmental profitability for S1. Besides, for the alternative S1, the ORC cycle has an acceptable economic profitability for a net power of 358.4 kW at a temperature of 110 °C, while for S2, this profitability starts being attractive for a power 2.65 times greater than S1 and with a temperature higher than 135 °C. In conclusion, the above represents an area of opportunity and a considerable advantage for the implementation of the ORC in the recovery of waste heat from flash–binary geothermal power plants.
Characteristics of Real-World Gaseous Emissions from Construction Machinery
Authors: Lee Dong In , Park Junhong , Shin Myunghwan , Lee Jongtae , Park Sangki
Published: December 2022

In Korea’s air pollutant inventory, construction machinery is a major emission source in the non-road sector. Since 2004, the Korean government has introduced and reinforced emission regulations to reduce the air pollutants emitted from their diesel engines. Since the engine dynamometer test method used in emission regulations has limitations in reflecting emission characteristics under the diverse working conditions of construction machinery, it is necessary to examine the effectiveness of emission regulations and the validity of the emission factors applied as inputs to the air pollutants inventory. This could be done by evaluating engine operation and emission characteristics under real-world working conditions. In this study, 14 units were selected among the excavators, wheel loaders, and forklifts that represent approximately 90% of the registered construction machines in Korea. They were equipped with a portable emission measurement system (PEMS) to measure gaseous emissions and collect engine data under various real-world working conditions. With the reinforcement of emission regulations for the construction machinery from K-tier3 to K-tier4 in Korea, exhaust after-treatment technologies, such as selective catalytic reduction and diesel oxidation catalyst, were applied. Real world NOx was reduced by approximately 83%, and THC 77% and CO by 73%, respectively. Real world NOx + THC of the K-tier3 machines exceeded the laboratory emission limit, but the K-tier4 machines considerably improved, 20% for excavator (124 kW), 61% for excavator (90 kW), 90% for wheel loader (202 kW) and 21% for Fork-lift (55 kW), despite some differences. The emission factors applied to the air pollutant inventory have been developed using the engine dynamometer test method, but they were considerably underestimated compared with emissions under real-world working conditions. The difference was even larger for the K-tier4 machines. In this study, the possibility of developing emission factor equations that use the engine load factor as a parameter was confirmed by using the engine work 1 g/kW·h segment moving averaging window (MAW) method.
Potential Geographical Distribution of Medicinal Plant Ephedra sinica Stapf under Climate Change
Authors: Zhang Kai , Liu Zhongyue , Abdukeyum Nurbiya , Ling Yibo
Published: December 2022

Ephedra sinica Stapf is an important traditional medicinal plant. However, in recent years, due to climate change and human activities, its habitat area and distribution area have been decreasing sharply. In order to provide better protection for E. sinica, it is necessary to study the historical and future potential zoning of E. sinica. The maximum entropy model (MaxEnt) was used to simulate the potential geographical distribution patterns of E. sinica under historical and future climatic conditions simulated using two Shared Socio-economic Pathways. The main results were also analyzed using the jackknife method and ArcGIS. The results showed that: (1) the potential suitable distribution area of E. sinica in China is about 29.18 × 105 km2—high-suitable areas, medium-suitable areas, and low-suitable areas cover 6.38 × 105 km2, 8.62 × 105 km2, 14.18 × 105 km2, respectively—and E. sinica is mainly distributed in Inner Mongolia; (2) precipitation and temperature contribute more to the distribution of E. sinica; (3) under two kinds of SSPs, the total suitable area of E. sinica increased significantly, but the differences between 2021–2040, 2041–2060, 2061–2080, and 2081–2100 are not obvious; (4) the barycentre of E. sinica moves from the historical position to its southwest. The results show that E. sinica can easily adapt to future climates well, and its ecological value will become more important. This study provides scientific guidance for the protection, management, renewal and maintenance of E. sinica.