bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation and Design of Large-Scale Solar Adsorption Cooling Systems Based on Energetic, Economic and Environmental Performance
Authors: Bawazir Abdullah , Friedrich Daniel
Published: March 2022

In hot and dry regions such as the Gulf Cooperation Council (GCC) countries, the cooling demand is often responsible for more than 70% of electricity consumption, which places a massive strain on the electricity grid and leads to significant emissions. Solar thermal driven Silica-Gel/Water adsorption chillers, used for space cooling, could provide low carbon emission cooling and reduce the reliance on grid electricity. However, a meticulous design is required to make this both economically and environmentally beneficial. This paper aims to evaluate the solar thermal adsorption chiller performance based on large-scale cooling demand through a TRNSYS simulation for 1 year of operation. The proposed system consists of two main parts: first, the solar loop with evacuated tube solar collectors; and second, the adsorption cooling system with a silica-gel/water adsorption chiller. A neighbourhood of 80 typical 197 m2 villas in Riyadh, the capital city of the Kingdom of Saudi Arabia (KSA), was taken as a case study. The solar adsorption cycle’s performance has been compared to the conventional vapour compression cycle in terms of energy, economic and environmental performance. In addition, a parametric study has been performed for the main design parameters. Results reveal that the system can reach a solar fraction of 96% with solar collector area of 5500 m2 and a storage tank volume between 350 and 400 m3. Furthermore, the annual energy cost can be reduced by 74% for the solar adsorption system compared to the conventional vapour compression cycle. Meanwhile, the CO2 saving percentage for the solar adsorption cycle was approximately 75% compared to the conventional vapour compression cycle. Carefully designed solar thermal cooling systems can reduce greenhouse gas emissions while covering a large scale of cooling demands. This can reduce the strain on the electricity grid as well as greenhouse gas emissions.
Spatial Patterns and Drivers of Soil Chemical Properties in Typical Hickory Plantations
Authors: Sun Mengjiao , Hou Enqing , Wu Jiasen , Huang Jianqin , Huang Xingzhao , Xu Xiaoniu
Published: March 2022

Soil nutrients play critical roles in regulating and improving the sustainable development of economic forests. Consequently, an elucidation of the spatial patterns and drivers of soil nutrients in these forests is fundamental to their management. For this study, we collected 314 composite soils at a 0–30 cm depth from a typical hickory plantation in Lin’an, Zhejiang Province, China. We determined the concentrations of macronutrients (i.e., soil organic carbon, available potassium, available phosphorus, available sulfur, and hydrolyzed nitrogen) and micronutrients (i.e., soil available boron, iron, manganese, zinc, and copper) of the soils. We employed random forest analysis to quantify the relative importance of factors affecting soil nutrients to predict the concentrations, which could then be extrapolated to the entire hickory region. Random forest models explained 43–80% of the variations in soil nutrient concentrations. The mean annual temperature, mean annual precipitation, and altitude were key predictors of soil macronutrient and micronutrient concentrations. Moreover, slope and parent material were important predictors of soil nutrients concentrations. Distinct spatial patterns of soil nutrient concentrations were driven by climate, parent material, and topography. Our study highlights the various environmental controls over soil macronutrient and micronutrient concentrations, which have significant implications for the management of soil nutrients in hickory plantations.
Editorial: Special Issue “Implementation of Digital Technologies on Beverage Fermentation”
Authors: Gonzalez Viejo Claudia , Fuentes Sigfredo
Published: March 2022

In the food and beverage industries, implementing novel methods using digital technologies such as artificial intelligence (AI), sensors, robotics, computer vision, machine learning (ML), and sensory analysis using augmented reality (AR) has become critical to maintaining and increasing the products’ quality traits and international competitiveness, especially within the past five years [...]
Modeling of Wood Surface Ignition by Wildland Firebrands
Authors: Matvienko Oleg , Kasymov Denis , Loboda Egor , Lutsenko Anastasia , Daneyko Olga
Published: March 2022

The probability of structural ignition is dependent both on physical properties of materials and the fire exposure conditions. In this study, the effect of firebrand characteristics (i.e., firebrand size, number of firebrands) on wood ignition behavior was considered. Mathematical modeling and laboratory experiment were conducted to better understand the conditions of wood ignition by a single or group of firebrands with different geometry. This model considers the heat exchange between the firebrands, wood layer and the gas phase, moisture evaporation in the firebrands and the diffusion gases of water vapor in the pyrolysis zone. In order to test and verify the model, a series of experiments to determine probability and conditions for ignition of wood-based materials (plywood, oriented strand board, chipboard) caused by wildland firebrands (pine twigs with a diameter of 6–8 mm and a length of 40 ± 2 mm) were conducted. The experiments investigated the firebrand impact on the wood layer under different parameters, such as firebrand size and quantity, wind speed, and type of wood. The results of experiments showed that the increase in wind speed leads to the increase in probability of wood ignition. Based on the received results, it can be concluded that the ignition curve of wood samples by firebrands is nonlinear and depends on the wind speed and firebrand size as well as their quantity. At the same time, there is no ignition of wood samples in the range of wind speed of 0–1 m/s. The ignition of wood is possible with a decrease in the distance between the firebrands with a decrease in the firebrand length. This result agrees more closely with the model.
Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis
Authors: Hu Shuyang , Li Yuwei , Wang Boran , Yin Lijun , Jia Xin
Published: March 2022

The growth of Vibrio bacteria is affected by environmental conditions, and unfavorable conditions will produce different degrees of stress on Vibrio. The cells respond to the stress on the bacteria through changes in biological characteristics and transcriptomes. To study the effect of NaCl concentration on Vibrio brasiliensis, we have determined the biological characteristics of the 0%, 1%, 2%, 3%, 5%, and 7% NaCl concentrations cultured V. brasiliensis to research the salt stress to bacteria. We found that the biological properties of V. brasiliensis cultured with different NaCl concentrations were different, and the expression of outer membrane proteins of V. brasiliensis changed when it was grown under different NaCl concentrations. When bacteria cultured in higher NaCl concentrations (3%, 5% and 7% NaCl), the sodium-type flagellar protein MotY was found. Finally, the transcriptome analysis of V. brasiliensis cultured with 0% NaCl and 7% NaCl was carried out to find out the differentially expressed genes. We found that the same gene have opposite up-regulated and down-regulated expression in two treatments, indicating that these types of genes are regulated different in low and high osmotic stress.
Synthesis and Characterization of Conjugated Hyaluronic Acids. Application to Stability Studies of Chitosan-Hyaluronic Acid Nanogels Based on Fluorescence Resonance Energy Transfer
Authors: Malytskyi Volodymyr , Moreau Juliette , Callewaert Maité , Henoumont Céline , Cadiou Cyril , Feuillie Cécile , Laurent Sophie , Molinari Michael , Chuburu Françoise
Published: March 2022

Hyaluronic acid (HA) was functionalized with a series of amino synthons (octylamine, polyethylene glycol amine, trifluoropropyl amine, rhodamine). Sodium hyaluronate (HAs) was first converted into its protonated form (HAp) and the reaction was conducted in DMSO by varying the initial ratio (−NH2 (synthon)/COOH (HAp)). HA derivatives were characterized by a combination of techniques (FTIR, 1H NMR, 1D diffusion-filtered 19F NMR, DOSY experiments), and degrees of substitution (DSHA) varying from 0.3% to 47% were determined, according to the grafted synthon. Nanohydrogels were then obtained by ionic gelation between functionalized hyaluronic acids and chitosan (CS) and tripolyphosphate (TPP) as a cross-linker. Nanohydrogels for which HA and CS were respectively labeled by rhodamine and fluorescein which are a fluorescent donor-acceptor pair were subjected to FRET experiments to evaluate the stability of these nano-assemblies.
Screening the Significant Hub Genes by Comparing Tumor Cells, Normoxic and Hypoxic Glioblastoma Stem-like Cell Lines Using Co-Expression Analysis in Glioblastoma
Authors: Güven Emine , Afzal Muhammad , Kazmi Imran
Published: March 2022

Glioblastoma multiforme (GBM) is categorized by rapid malignant cellular growth in the central nervous system (CNS) tumors. It is one of the most prevailing primary brain tumors, particularly in human male adults. Even though the combination therapy comprises surgery, chemotherapy, and adjuvant therapies, the survival rate is on average 14.6 months. Glioma stem cells (GSCs) have key roles in tumorigenesis, progression, and counteracting chemotherapy and radiotherapy. In our study, firstly, the gene expression dataset GSE45117 was retrieved and differentially expressed genes (DEGs) were spotted. The co-expression network analysis was employed on DEGs to find the significant modules. The most significant module resulting from co-expression analysis was the turquoise module. The turquoise module related to the tumor cells, hypoxia, normoxic treatments of glioblastoma tumor (GBT), and GSCs were screened. Sixty-one common genes in the turquoise module were selected generated through the co-expression analysis and protein–protein interaction (PPI) network. Moreover, the GO and KEGG pathway enrichment results were studied. Twenty common hub genes were screened by the NetworkAnalyst web instrument constructed on the PPI network through the STRING database. After survival analysis via the Kaplan–Meier (KM) plotter from The Cancer Genome Atlas (TCGA) database, we identified the five most significant hub genes strongly related to the progression of GBM. We further observed these five most significant hub genes also up-regulated in another GBM gene expression dataset. The protein–protein interaction (PPI) network of the turquoise module genes was constructed and a KEGG pathway enrichments study of the turquoise module genes was performed. The VEGF signaling pathway was emphasized because of the strong link with GBM. A gene–disease association network was further constructed to demonstrate the information of the progression of GBM and other related brain neoplasms. All hub genes assessed through this study would be potential markers for the prognosis and diagnosis of GBM.
The ATENción Plena en Enfermedad de Alzheimer (ATENEA—Mindfulness in Alzheimer’s Disease) Program for Caregivers: Study Protocol for a Randomized Controlled Trial
Authors: Sánchez-Pérez Alicia , Mendialdua-Canales Daniel , Hurtado-Pomares Miriam , Peral-Gómez Paula , Juárez-Leal Iris , Espinosa-Sempere Cristina , Fernández-Pires Paula , Zango-Martín Inmaculada , Abellán-Miralles Inmaculada , López-González Pablo , Valera-Gran Desirée , Navarrete-Muñoz Eva-María
Published: March 2022

A person affected by Alzheimer’s disease (AD) gradually loses the ability to perform activities of daily living and becomes dependent on caregivers, thereby having a negative impact on the caregivers’ quality of life. There is evidence that suggests that interventions aimed at caregivers, such as mindfulness, may be effective at reducing this burden and emotional issues, such as depression and anxiety, and improving their quality of life. However, there is a lack of consistency in the findings and conclusions remain tentative. In addition, as neuropsychiatric symptoms (NPSs) of AD are major determinants of the caregiver’s burden, these interventions should examine the relationship between these symptoms and caregiver outcomes. Importantly, to improve the design of therapeutic interventions for caregivers and complement the treatment of AD, aspects related to occupational performance and the participation of people with AD and their caregivers should also be considered. Therefore, this study will aim to examine first, the effects of a mindfulness-based program designed for caregivers on NPSs of AD and caregivers’ anxiety and depression; second, the effects of this program on patients’ functional capacity, cognitive performance, executive functions, and quality of life, and on caregivers’ burden, quality of life, occupational balance, executive functions, psychological wellbeing, and self-compassion. We believe that the findings of this study will have significant implications for future healthcare strategies focused on improving the quality of life and wellbeing of caregivers.
Acute Stroke Care during COVID-19: National Data
Authors: Miękisiak Grzegorz , Pettersson Samuel D. , Szarek Dariusz , Morasiewicz Piotr , Fercho Justyna , Adamski Stanisław , Kipiński Lech , Szmuda Tomasz
Published: March 2022

(1) Background: The pandemic of COVID-19 and subsequent lockdown strategies had a profound impact on many aspects of everyday life. During this time the world faced the unprecedented crisis of healthcare disrupting timely care delivery. This study was designed to evaluate the impact of the pandemic on the acute treatment of stroke in Poland. (2) Methods: The national data on hospitalizations with stroke as a primary diagnosis were obtained from the National Health Fund of Poland. Poisson regression was used to determine the significance of the change in hospital admissions. The differences between proportions were analyzed using the “N-1” Chi-squared test. (3) Results: During the COVID-19 period, the number of hospitalizations dropped by 8.28% with a monthly nadir of 22.02 in April. On a monthly scale during 2020, the greatest decrease was 22.02%. The thrombolysis ratio was also affected, with the highest monthly drop of 15.51% in November. The overall number of in-hospital deaths did not change. (4) Conclusions: The pandemic caused a serious disruption of the acute care of stroke. There is no evidence that the quality of care was seriously compromised.
Flavored Tobacco Sales Restrictions Reduce Tobacco Product Availability and Retailer Advertising
Authors: Holmes Louisa M. , Lempert Lauren Kass , Ling Pamela M.
Published: March 2022

Objective: This study examined differences in the availability and advertising of flavored tobacco products before and after flavored tobacco sales restrictions were enacted in Alameda and San Francisco Counties in California. Main outcome measures: Data were collected from a sample of tobacco retailers in Alameda and San Francisco Counties at two time points: 2015, before flavored tobacco policies were enacted, and in 2019–2020, after some cities had enacted policies. Retailers were separated by city into Category 1 (n = 442)—retailers in cities that enacted a flavored tobacco policy between the two data collection periods, and Category 2 (n = 89), those that had not. Means comparison tests were conducted to evaluate significant differences over time and by category. Results: There was significantly reduced availability of menthol cigarettes, flavored little cigars, smokeless tobacco, vape pens, and Blu brand menthol e-cigarettes between 2015 and 2020 in Category 1 retailers. Category 2 retailers had reduced availability only for Blu menthol e-cigarettes and demonstrated an increase in smokeless tobacco availability. Exterior store advertising for cigarettes, little cigars, cigars, and e-cigarettes also decreased significantly in Category 1 cities relative to Category 2 cities; 8.1% of Category 1 stores were advertising flavored tobacco products in 2019–2020 compared to 36.2% of Category 2 stores. There was also a 78% reduction in flavored ads between 2015–2019 in Category 1 cities compared to a 38% decrease in Category 2 cities. Tobacco advertising inside Category 2 stores increased. Finally, Category 2 cities had significantly greater availability of cigalikes, mod or tank vapes, flavored e-cigarettes, and e-liquids compared to Category 1 cities. Conclusions. Comprehensive flavored sales restriction policies reduce flavored tobacco availability and tobacco advertising, which may help prevent youth tobacco initiation and exposure.
COVID-19-Vaccination-Induced Myocarditis in Teenagers: Case Series with Further Follow-Up
Authors: Puchalski Mateusz , Kamińska Halszka , Bartoszek Marta , Brzewski Michał , Werner Bożena
Published: March 2022

Presently, the whole globe is struggling the tough challenge of the COVID-19 pandemic. Vaccination remains the most effective and safe COVID-19 weapon for adults and in the paediatric population. Aside from possible mild and moderate post-vaccination side effects, more severe side effects may occur. We retrospectively analysed a group of 5 teenagers aged from 15 to 17 years with obesity/overweight (BMI ranging from 24.8 to 30) who presented typical myocarditis symptoms following the first or second dose (3 and 2 patients, respectively) of the COVID-19 vaccine. In the whole study group, a significant increase in troponin serum concentration was observed (1674–37,279.6 ng/L) with a further quick reduction within 3–4 days. In all patients, ST segments elevation or depression with repolarisation time abnormalities in electrocardiography were noticed. Chest X-ray results were within normal limits. Echocardiography showed normal left ventricular diameter (47–56.2 mm) with ejection fraction between 61–72%. All patients were diagnosed with myocarditis based on cardiac magnetic resonance (CMR) imaging. During further hospitalisation, swift clinical improvement was notable. Follow-up in the whole study group was obtained after 106–134 days from initial CMR, revealing no myocarditis symptoms, proper troponin level, and no ECG or echocardiographic abnormalities. At the same time, persistent myocardium injury features were detected in the whole study group, including ongoing myocarditis. COVID-19-vaccine-induced myocarditis seems to be a mild disease with fast clinical recovery, but the complete resolution of the inflammatory process may last over 3 months. Further follow-up and investigation for assessing subsequent implications and long-term COVID-19-vaccine-induced myocarditis is required.
The Negative Effects of Long Time Physical Activity Calorie Equivalent Labeling on Purchase Intention for Unhealthy Food
Authors: Huang Yuanhao , Yang Xiaoke , Chen Qian
Published: March 2022

(1) Background: Obesity has become a global epidemic that arouse much attention from governments, companies and scholar. Physical activity calorie equivalent (PACE) labels are introduced as a more effective nudge invention on less-calorie ordering. However, the effects of PACE labels are controversial in previous literature, thus, the research objective is to explore the effects of different PACE labels and furtherly to explore the underlying psychological mechanism; (2) Methods: Across four scenario-based experiments, involving potato chips, chocolate and cookies, this study manipulated the three calorie-information labeling (standard calorie label, long time PACE and short time PACE labels). Meanwhile, the mediating mechanism of the effects involving anticipatory guilt and the moderation effects between consumers’ future self-continuity and PACE labels are also measured; (3) Results: Results show that compared with the short time PACE and calorie labels, the longtime PACE labels have more negative influence on consumers’ purchase intention for unhealthy food. What’s more, the anticipatory guilt has negative effect of PACE labels as consumers are often prone to feeling guilty in the process of unhealthy food consumption. In addition, individuals with high future self-continuity have higher self-control and take more consideration of future outcomes, they are reluctant to choose unhealthy food than others; (4) Conclusions: Unhealthy food with a long time PACE label has more negative effect on consumers’ purchase intention rather than a short time PACE label. At the same time, companies that produce healthy foods should actively participate in the movement to label calories through the PACE labels.
Examining the Effects of Installed Capacity Mix and Capacity Factor on Aggregate Carbon Intensity for Electricity Generation in China
Authors: Ma Shiping , Liu Qianqian , Zhang Wenzhong
Published: March 2022

Promoting technological advancements and energy transitions in electricity generation are crucial for achieving carbon reduction goals. Some studies have examined the effectiveness of these measures by analysing the driving forces of “aggregate carbon intensity” (ACI) change. However, only a few studies have considered the effect of the installed capacity mix and capacity factor. Moreover, such analysis has never been applied at China’s provincial level after 2015. To alleviate this gap, our study applied a temporal and multi-regional spatial IDA-LMDI model to analyse the driving factors of ACI changes and disparities among the provinces of China from 2005 to 2019. The model notably includes the effects of the installed capacity mix, thermal capacity factor, and overall capacity factor. The analysis revealed that the decline in China’s ACI was diminished after 2015, while an ACI rebound was identified in five provinces. The changes in the ACI from 2015 to 2019 were mainly driven by the effect of the installed capacity mix rather than by the thermal efficiency and thermal capacity factor. The overall capacity factor was the only factor with a negative impact on the ACI change. We also found that its combined effect with the thermal capacity factor on increasing ACI can offset the effect of the installed capacity mix by reducing the ACI in provinces with significant additions of renewable energy installed capacity. The analysis of the influencing factors on the provincial ACI differences revealed that the share of hydropower installed capacity was significant. Moreover, the thermal efficiency and thermal capacity factor both played key roles in the ACI disparities in northeast, northwest, and central China. Overall, this study paves the way for data-driven measures of China’s carbon peak and carbon neutrality goals by improving the capacity factor of wind and solar power, leveraging the critical impact of hydropower, and narrowing the differences in the thermal power sector among provinces.
Sexual Assault Is the Biggest Risk Factor for Violence against Women in Taiwan—A Nationwide Population Cohort Study from 2000 to 2015
Authors: Chwo Miao-Ju , Huang Shi-Hao , Huang Yao-Ching , Lin Iau-Jin , Yu Chia-Peng , Chung Chi-Hsiang , Chien Wu-Chien , Sun Chien-An , Wu Gwo-Jang
Published: March 2022

Objective: To understand the main types of risk of violence against women in Taiwan. Materials and methods: This study used the outpatient, emergency, and hospitalization data of 2 million people in the National Health Insurance sample from 2000 to 2015. The International Classification of Diseases, Ninth Revision diagnostic N-codes 995.5 (child abuse) and 995.8 (adult abuse) or E-codes E960–E969 (homicide and intentional injury by others) were defined as the case study for this study, and the risks of first violent injury for boys and girls (0–17 years old), adults (18–64 years old), and elders (over 65 years old) were analyzed. Logistic regression analysis was used for risk comparison. A p value of <0.05 was considered significant. Results: The proportion of women (12–17.9 years old) who were sexually assaulted was 2.71 times that of women under the age of 12, and the risk of sexual assault for girls and adult women was 100 times that of men. Girls who were insured as labor insurance, farmers, members of water conservancy and fishery associations, low-income households, and community insured population (public insurance as the reference group) were significantly more likely to seek medical treatment from sexual assault than adult women. Among them, the risk was greatest for girls from low-income households (odds ratio = 10.74). Conclusion: Women are at higher risk of sexual assault than men regardless of whether they are children or adults, and the highest risk is for women in senior high schools, especially for girls from low-income households. Therefore, the protection of women’s personal autonomy is the direction that the government and people from all walks of life need to continue to strive for. Especially for high school students from low-income households, protection must be strengthened through education, social work, and police administration.
Exercise Intervention Changes the Perceptions and Knowledge of Non-Communicable Disease Risk Factors among Women from a Low-Resourced Setting
Authors: Makamu-Beteck Sweetness Jabulile , Moss Sarah Johannah , Watson Francois Gerald , Cameron Melainie
Published: March 2022

We employed the Health Belief Model (HBM) as a theoretical lens to explore the influence of an exercise intervention on the perceptions and knowledge of modifiable risk factors for non-communicable diseases (NCDs) among women from a low-resource setting in South Africa. We used a mixed-methods design, gathering qualitative and quantitative data at baseline (n = 95) and again after 12 weeks (n = 55) and 24 weeks (n = 44) of an exercise intervention. Qualitative data consisted of focus group discussions exploring the knowledge and perceptions of modifiable risk factors for NCDs at the three time points. We collected quantitative measurements of modifiable risk factors for NCDs (waist-to-hip ratio, body mass index, blood pressure, peripheral blood glucose, and cholesterol) as well as objective physical activity (PA) data over seven consecutive days. Surveys on coronary heart disease and PA knowledge were conducted at all three time points. Qualitative findings indicated that health exposures and cultural traditions influenced the participant’s perceptions about PA and NCDs. Waist circumference significantly decreased at 12 weeks compared to baseline MD = 4.16, p < 0.001. There was significant improvement at 12 weeks, compared to baseline, MD = 0.59, p = 0.009 for PA knowledge, and MD = 0.68, p = 0.003 for heart disease knowledge. There were reductions from baseline to 24 weeks in diastolic blood pressure (MD = 4.97, p = 0.045), waist circumference (MD = 2.85, p = 0.023) and BMI (MD = 0.82, p = 0.004). Significant heart disease knowledge improvements were found at 24 weeks compared to baseline (MD = 0.75, p < 0.001). Supervised exercise positively influenced Black African females′ health behaviours by understanding cultural perceptions of modifiable risk factors for NCDs.
Work-Time Control and Exhaustion: Internal Work-to-Home Interference and Internal Home-to-Work Interference as Mediators
Authors: Vieten Laura , Wöhrmann Anne Marit , Michel Alexandra
Published: March 2022

Strong work-time control (WTC) has been linked to reduced employee exhaustion, with work-to-home interference as an underlying mechanism. In this study, we aimed to investigate the mediation effect of both directions of internal work–home interference, namely internal work-to-home interference (IWHI) and internal home-to-work interference (IHWI). The analysis is based on data from the 2015, 2017, and 2019 BAuA-Working Time Survey, a representative German panel study. Cross-lagged panel models were estimated separately for IWHI and IHWI, based on the balanced panel (n = 3390). We investigated the hypothesized indirect as well as potential direct, reversed, and reciprocal effects of the constructs. WTC had a small but significant indirect effect on exhaustion via IWHI. Contrary to assumptions, WTC positively affected IHWI. Unexpectedly, there was no significant effect of IHWI on exhaustion. Hence, only IWHI was identified to mediate WTC’s effect on exhaustion. This implies that WTC helps employees avoid exhaustion from psychological preoccupation with work during free time. In addition, analyses suggested reversed and reciprocal relationships between the investigated constructs. Further investigation is needed to explore the role of psychological preoccupation with private matters during work time in the context of WTC and employee well-being.
Spatiotemporal Evolution and Driving Mechanism of “Production-Living-Ecology” Functions in China: A Case of Both Sides of Hu Line
Authors: Chen Yu , Zhu Mengke
Published: March 2022

In order to explore the spatiotemporal evolution of land use function and its driving factors in China, taking both sides of the Hu Line as an example, we used Exploratory Spatial Data Analysis and Geographically Weighted Regression methods to reveal dynamic evolution law, spatial characteristics and influencing factors of the “Production-Living-Ecology” functions of 288 prefecture-level cities on both sides of the Hu Line. The results show that: (1) In the temporal dimension, the coordination of “Production-Living-Ecology” functions of land use in China has been improved, and the Hu Line can be roughly used as the boundary of China’s territorial space use. (2) In the spatial dimension, there is a significant positive spatial correlation between “Production-Living-Ecology” functions of land use in China, and the coordination gap between “Production-Living-Ecology” functions of land use on both sides of the Hu Line is gradually narrowing. (3) In terms of influencing mechanism, the coordination of “Production-Living-Ecology” functions is mainly driven by internal factors and is supplemented by external ones. The influence pattern of most driving factors is consistent with the layout characteristics of “strong east and weak west” of the Hu Line.
How to Differentiate General Toxicity-Related Endocrine Effects from Endocrine Disruption: Systematic Review of Carbon Disulfide Data
Authors: Printemps Nathalie , Le Magueresse-Battistoni Brigitte , Mhaouty-Kodja Sakina , Viguié Catherine , Michel Cécile
Published: March 2022

This review provides an overview of the assessment of the endocrine disrupting (ED) properties of carbon disulfide (CS2), following the methodology used at the European level to identify endocrine disruptors. Relevant in vitro, in vivo studies and human data are analyzed. The assessment presented here focuses on one endocrine activity, i.e., thyroid disruption, and two main adverse effects, neurotoxicity and cardiotoxicity. The data available on the different ED or non-ED modes of action (MoA), known to trigger these adverse effects, are described and the strength of evidence of the different MoA is weighted. We conclude that the adverse effects could be due to systemic toxicity rather than endocrine-mediated toxicity. This assessment illustrates the scientific and regulatory challenges in differentiating a specific endocrine disruption from an indirect endocrine effect resulting from a non-ED mediated systemic toxicity. This issue of evaluating the ED properties of highly toxic and reactive substances has been insufficiently developed by European guidance so far and needs to be further addressed. Finally, this example also raises questions about the capacity of the technics available in toxicology to address such a complex issue with certainty.
From Cancer Therapy to Winemaking: The Molecular Structure and Applications of β-Glucans and β-1, 3-Glucanases
Authors: Caseiro Catarina , Dias Joana Nunes Ribeiro , de Andrade Fontes Carlos Mendes Godinho , Bule Pedro
Published: March 2022

β-glucans are a diverse group of polysaccharides composed of β-1,3 or β-(1,3-1,4) linked glucose monomers. They are mainly synthesized by fungi, plants, seaweed and bacteria, where they carry out structural, protective and energy storage roles. Because of their unique physicochemical properties, they have important applications in several industrial, biomedical and biotechnological processes. β-glucans are also major bioactive molecules with marked immunomodulatory and metabolic properties. As such, they have been the focus of many studies attesting to their ability to, among other roles, fight cancer, reduce the risk of cardiovascular diseases and control diabetes. The physicochemical and functional profiles of β-glucans are deeply influenced by their molecular structure. This structure governs β-glucan interaction with multiple β-glucan binding proteins, triggering myriad biological responses. It is then imperative to understand the structural properties of β-glucans to fully reveal their biological roles and potential applications. The deconstruction of β-glucans is a result of β-glucanase activity. In addition to being invaluable tools for the study of β-glucans, these enzymes have applications in numerous biotechnological and industrial processes, both alone and in conjunction with their natural substrates. Here, we review potential applications for β-glucans and β-glucanases, and explore how their functionalities are dictated by their structure.
Molecular Dynamics Simulations of Transmembrane Cyclic Peptide Nanotubes Using Classical Force Fields, Hydrogen Mass Repartitioning, and Hydrogen Isotope Exchange Methods: A Critical Comparison
Authors: Conde Daniel , Garrido Pablo F. , Calvelo Martín , Piñeiro Ángel , Garcia-Fandino Rebeca
Published: March 2022

Self-assembled cyclic peptide nanotubes with alternating D- and L-amino acid residues in the sequence of each subunit have attracted a great deal of attention due to their potential for new nanotechnology and biomedical applications, mainly in the field of antimicrobial peptides. Molecular dynamics simulations can be used to characterize these systems with atomic resolution at different time scales, providing information that is difficult to obtain via wet lab experiments. However, the performance of classical force fields typically employed in the simulation of biomolecules has not yet been extensively tested with this kind of highly constrained peptide. Four different classical force fields (AMBER, CHARMM, OPLS, and GROMOS), using a nanotube formed by eight D,L-α-cyclic peptides inserted into a lipid bilayer as a model system, were employed here to fill this gap. Significant differences in the pseudo-cylindrical cavities formed by the nanotubes were observed, the most important being the diameter of the nanopores, the number and location of confined water molecules, and the density distribution of the solvent molecules. Furthermore, several modifications were performed on GROMOS54a7, aiming to explore acceleration strategies of the MD simulations. The hydrogen mass repartitioning (HMR) and hydrogen isotope exchange (HIE) methods were tested to slow down the fastest degrees of freedom. These approaches allowed a significant increase in the time step employed in the equation of the motion integration algorithm, from 2 fs up to 5–7 fs, with no serious changes in the structural and dynamical properties of the nanopores. Subtle differences with respect to the simulations with the unmodified force fields were observed in the concerted movements of the cyclic peptides, as well as in the lifetime of several H-bonds. All together, these results are expected to contribute to better understanding of the behavior of self-assembled cyclic peptide nanotubes, as well as to support the methods tested to speed up general MD simulations; additionally, they do provide a number of quantitative descriptors that are expected to be used as a reference to design new experiments intended to validate and complement computational studies of antimicrobial cyclic peptides.
ICA1L Is Associated with Small Vessel Disease: A Proteome-Wide Association Study in Small Vessel Stroke and Intracerebral Haemorrhage
Authors: Cullell Natalia , Gallego-Fábrega Cristina , Cárcel-Márquez Jara , Muiño Elena , Llucià-Carol Laia , Lledós Miquel , Martín-Campos Jesús M. , Molina Jessica , Casas Laura , Almeria Marta , Fernández-Cadenas Israel , Krupinski Jerzy
Published: March 2022

Small vessel strokes (SVS) and intracerebral haemorrhages (ICH) are acute outcomes of cerebral small vessel disease (SVD). Genetic studies combining both phenotypes have identified three loci associated with both traits. However, the genetic cis-regulation at the protein level associated with SVD has not been studied before. We performed a proteome-wide association study (PWAS) using FUSION to integrate a genome-wide association study (GWAS) and brain proteomic data to discover the common mechanisms regulating both SVS and ICH. Dorsolateral prefrontal cortex (dPFC) brain proteomes from the ROS/MAP study (N = 376 subjects and 1443 proteins) and the summary statistics for the SVS GWAS from the MEGASTROKE study (N = 237,511) and multi-trait analysis of GWAS (MTAG)-ICH–SVS from Chung et al. (N = 240,269) were selected. We performed PWAS and then a co-localization analysis with COLOC. The significant and nominal results were validated using a replication dPFC proteome (N = 152). The replicated results (q-value < 0.05) were further investigated for the causality relationship using summary data-based Mendelian randomization (SMR). One protein (ICA1L) was significantly associated with SVS (z-score = −4.42 and p-value = 9.6 × 10−6) and non-lobar ICH (z-score = −4.8 and p-value = 1.58 × 10−6) in the discovery PWAS, with a high co-localization posterior probability of 4. In the validation PWAS, ICA1L remained significantly associated with both traits. The SMR results for ICA1L indicated a causal association of protein expression levels in the brain with SVS (p-value = 3.66 × 10−5) and non-lobar ICH (p-value = 1.81 × 10−5). Our results show that the association of ICA1L with SVS and non-lobar ICH is conditioned by the cis-regulation of its protein levels in the brain.
Knee and Peri-Knee Tissues of Post Mortem Donors Are Strategic Sources of Mesenchymal Stem/Stromal Cells for Regenerative Procedures
Authors: Haring Gregor , Zupan Janja
Published: March 2022

Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of CD146 in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.
Tailoring ZE21B Alloy with Nature-Inspired Extracellular Matrix Secreted by Micro-Patterned Smooth Muscle Cells and Endothelial Cells to Promote Surface Biocompatibility
Authors: Liu Changsheng , Chen Lan , Zhang Kun , Li Jingan , Guan Shaokang
Published: March 2022

Delayed surface endothelialization is a bottleneck that restricts the further application of cardiovascular stents. It has been reported that the nature-inspired extracellular matrix (ECM) secreted by the hyaluronic acid (HA) micro-patterned smooth muscle cells (SMC) and endothelial cells (EC) can significantly promote surface endothelialization. However, this ECM coating obtained by decellularized method (dECM) is difficult to obtain directly on the surface of degradable magnesium (Mg) alloy. In this study, the method of obtaining bionic dECM by micro-patterning SMC/EC was further improved, and the nature-inspired ECM was prepared onto the Mg-Zn-Y-Nd (ZE21B) alloy surface by self-assembly. The results showed that the ECM coating not only improved surface endothelialization of ZE21B alloy, but also presented better blood compatibility, anti-hyperplasia, and anti-inflammation functions. The innovation and significance of the study is to overcome the disadvantage of traditional dECM coating and further expand the application of dECM coating to the surface of degradable materials and materials with different shapes.
Gender Particularities and Prevalence of Atypical Clinical Presentation in Non-ST Elevation Acute Coronary Syndrome
Authors: Negrea Mihai Octavian , Zdrenghea Dumitru , Teodoru Minodora , Neamțu Bogdan , Cipăian Călin Remus , Pop Dana
Published: March 2022

Clinical presentation is one of the factors that can influence how quickly a patient with an acute coronary syndrome is treated, particularly if it is atypical. The purposes of this study are to explore gender-related differences in patients presenting with non-ST elevation acute coronary syndromes (NSTEACS) from the perspective of a series of common risk factors as well as treatment strategies and to evaluate the prevalence of atypical clinical presentation of NSTEACS in the study group. In addition, we explored the differences between the two entities that define NSTEACS: unstable angina (UA) and non-ST elevation myocardial infarction (NSTEMI). We conducted a retrospective study by reviewing discharge documents of patients admitted in the cardiology department of the Clinical Rehabilitation Hospital in Cluj-Napoca with NSTEACS between January 2014 and December 2015. We retrieved demographic data, clinical presentation and history, laboratory tests, and coronary angiography records as well as the implemented treatment strategies. Women in the study group were more frequently hypertensive than men (89.5% vs. 75.4%; p = 0.043), had a higher mean serum HDL cholesterol value (43 vs. 38 mg/dL p = 0.022), were more frequently diagnosed with microvascular coronary heart disease (32% vs. 9.8%, p = 0.036), and were more often treated conservatively (49.1% vs. 30.8%, p = 0.038), while men were significantly more prone to smoking than women (30.8% vs. 14%, p = 0.028) and had higher mean serum creatinine (1.2 vs. 0.8 mg/dL; p = 0.022) and uric acid values (6.9 vs. 6.2 mg/dL; p = 0.048). Out of the 122 included patients, 109 had documented information regarding symptoms. The prevalence of atypical presentation was 4.6% (95% CI 0.7–8.5%). In our study group, patients with UA had a more frequent history of cardiovascular ischemic diseases (77.4% vs. 56.7%, p = 0.015), the mean value for BUN was higher in NSTEMI patients compared to patients with UA (47 vs. 39 mg/dL, p = 0.038) and NSTEMI patients more frequently received interventional treatment compared to patients with UA (60% vs. 41.9%; p = 0.046).
Prevalence of Opioid Use Disorder among Patients with Cancer-Related Pain: A Systematic Review
Authors: Preux Céline , Bertin Marion , Tarot Andréa , Authier Nicolas , Pinol Nathalie , Brugnon David , Pereira Bruno , Guastella Virginie
Published: March 2022

Background: The opioid use disorder is an international public health problem. Over the past 20 years it has been the subject of numerous publications concerning patients treated for chronic pain other than cancer-related. Patients with cancer-related pain are also at risk of opioid use disorder. The primary objective of this literature review was to determine the prevalence of opioid use disorder in patients with cancer-related chronic pain. Its secondary objective was to identify the characteristics of these opioid users. Methods: This is a literature review of studies published over the last twenty years, from 1 January 2000 to 31 December 2020 identified by searching the three main medical databases: Pubmed, Cochrane, and Embase. A meta-analysis took account of between and within-study variability with the use of random-effects models estimated by the DerSimonian and Laird method. Results: The prevalence of opioid use disorder was 8% (1–20%) and of the risk of use disorder was 23.5% (19.5–27.8%) with I2 values of 97.8% and 88.7%, respectively. Conclusions: Further studies are now needed on the prevalence of opioid use disorder in patients treated for cancer-related chronic pain. A screening scale adapted to this patient population is urgently needed.
Current Treatment of Myasthenia Gravis
Authors: Alhaidar Mohammed K. , Abumurad Sumayyah , Soliven Betty , Rezania Kourosh
Published: March 2022

Myasthenia gravis (MG) is the most extensively studied antibody-mediated disease in humans. Substantial progress has been made in the treatment of MG in the last century, resulting in a change of its natural course from a disease with poor prognosis with a high mortality rate in the early 20th century to a treatable condition with a large proportion of patients attaining very good disease control. This review summarizes the current treatment options for MG, including non-immunosuppressive and immunosuppressive treatments, as well as thymectomy and targeted immunomodulatory drugs.
Robot-Assisted Autonomous Reduction of a Displaced Pelvic Fracture: A Case Report and Brief Literature Review
Authors: Ge Yufeng , Zhao Chunpeng , Wang Yu , Wu Xinbao
Published: March 2022

Displaced pelvic fracture is among the most complicated fractures in traumatic orthopedics, with high mortality and morbidity. Reduction is considered a complex procedure as well as a key part in surgical treatment. However, few robotic techniques have been employed in the reduction of pelvic fracture, despite the rapid advancement of technologies. Recently, we designed a robot surgery system specialized in the autonomous reduction of displaced pelvic fracture and applied it in the true patient for the first time. In this paper, we report its successful clinical debut in the surgery of a displaced pelvic fracture. Total surgery time was 110 min and an anatomic reduction was achieved. We then present a brief overview of the literature about reduction techniques in pelvic fracture and introduce related principles involved in our robot-assisted reduction system.
Reply to Chen et al. Improvements in Outcomes and Expanding Indications for the Commando Procedure. Comment on “Giambuzzi et al. Surgical Aortic Mitral Curtain Replacement: Systematic Review and Metanalysis of Early and Long-Term Results. J. Clin. Med. 2021, 10, 3163”
Authors: Giambuzzi Ilaria , Bonalumi Giorgia , Di Mauro Michele , Alamanni Francesco , Zanobini Marco
Published: March 2022

We would like to thank Lin Chen et al. [...]