bluuebunny/crossref_metadata_2025 huggingface dataset:
Should Age-Dependent Absolute Risk Thresholds Be Used for Risk Stratification in Risk-Stratified Breast Cancer Screening?
Authors: Pashayan Nora , Antoniou Antonis C. , Lee Andrew , Wolfson Michael , Chiquette Jocelyne , Eloy Laurence , Eisen Andrea , Stockley Tracy L. , Nabi Hermann , Brooks Jennifer D. , Dorval Michel , Easton Douglas F. , Knoppers Bartha Maria , Chiarelli Anna M. , Simard Jacques
Published: September 2021

In risk-stratified cancer screening, multiple risk factors are incorporated into the risk assessment. An individual’s estimated absolute cancer risk is linked to risk categories with tailored screening recommendations for each risk category. Absolute risk, expressed as either remaining lifetime risk or shorter-term (five- or ten-year) risk, is estimated from the age at assessment. These risk estimates vary by age; however, some clinical guidelines (e.g., enhanced breast cancer surveillance guidelines) and ongoing personalised breast screening trials, stratify women based on absolute risk thresholds that do not vary by age. We examine an alternative approach in which the risk thresholds used for risk stratification vary by age and consider the implications of using age-independent risk thresholds on risk stratification. We demonstrate that using an age-independent remaining lifetime risk threshold approach could identify high-risk younger women but would miss high-risk older women, whereas an age-independent 5-year or 10-year absolute risk threshold could miss high-risk younger women and classify lower-risk older women as high risk. With risk misclassification, women with an equivalent risk level would be offered a different screening plan. To mitigate these problems, age-dependent absolute risk thresholds should be used to inform risk stratification.
Quality of Life, Sleep Quality, Depression, Anxiety, Stress, Eating Habits, and Social Bounds in Nurses during the Coronavirus Disease 2019 Pandemic in Qatar (The PROTECTOR Study): A Cross-Sectional, Comparative Study
Authors: Nashwan Abdulqadir J. , Villar Ralph C. , Al-Qudimat Ahmad R. , Kader Nisha , Alabdulla Majid , Abujaber Ahmad A. , Al-Jabry Mahmood M. , Harkous Michel , Philip Anite , Ali Raed , Chandra Prem , Yassin Mohamed A. , Shraim Mujahed , Singh Kalpana
Published: September 2021

There have been numerous concerns regarding the physical and mental health of nurses during the COVID-19 pandemic. Stress, sleep deprivation, anxiety, and depression potentiated nurses’ vulnerability to poor eating habits. Aims and Objectives: The purpose of this study was to explore the differences between nurses’ characteristics with COVID-19 facility designation, and sleep quality, depression, anxiety, stress, eating habits, social bonds, and quality of life. Design: A cross-sectional, comparative study. Methods: An online survey was sent using the corporation’s email to nurses working in three hospitals in Qatar from September to December 2020. One of them is a designated COVID-19 facility. The sleep quality, depression, eating habits, social bonds, and quality of life were measured using The Insomnia Severity Index (ISI), Depression Anxiety and Stress Scale 21 (DASS-21), Emotional Eater Questionnaire (EEQ), Oslo Social Support Scale (OSSS-3), and the World Health Organization Quality of Life (WHOQOL-BREF), respectively. Results: A total of 200 nurses participated in the study (RR: 13.3%). No statistically significant association was found between designated facility (COVID-19 vs. not COVID-19) or nurses’ characteristics and ISI categories (OR 1.15; 95% CI 0.54, 2.44). Nurses working in COVID-19 facilities had increased odds of having higher EEQ categories by 2.62 times (95% CI 1.18, 5.83). Similarly, no statistically significant associations were found between any of the nurses’ characteristics and OSSS-3 categories. On the other hand, no statistically significant associations were found between any of the nurses’ characteristics and QOL domains except for the gender and social relationships’ domain. Conclusion: Overall, the quality of life of nurses in Qatar is on a positive level whether they are assigned to a COVID-19 facility or not. Although no significant difference was found with regard to the sleep quality, stress, anxiety, depression, and eating habits between nurses in a COVID-19 facility and in a non-COVID-19 facility, special interventions to diminish stressors need to be implemented and maintained.
The Impact of COVID-19 on Stock Market Returns in Vietnam
Authors: Hung Dao Van , Hue Nguyen Thi Minh , Duong Vu Thuy
Published: September 2021

This paper studies the impacts of COVID-19 on the performance of the Vietnamese Stock Market—a rapidly growing emerging market in a country that has to date successfully controlled the disease outbreak. The study uses a random-effect model (REM) on panel data of stock returns of 733 listed companies on both HOSE (the Ho Chi Minh Stock Exchange) and HNX (the Hanoi Stock Exchange) from 2 January 2020 to 13 December 2020. The study shows that the number of daily COVID-19 confirmed cases in Vietnam has a negative impact on stock returns of listed companies in the market. The impacts were more severe for the pre-lockdown and second-wave period, compared to impact for the lockdown period. The impacts also differed across sectors, with the financial sector being the most affected. With significant government control and influence over the bank-dominated financial system, the financial sector was expected to absorb some of the negative shocks hitting the real sector. Such expectations were reflected in the stock market movement during the pandemic.
Evaluation of the Terrestrial Ecosystem Model Biome-BGCMuSo for Modelling Soil Organic Carbon under Different Land Uses
Authors: Ostrogović Sever Maša Zorana , Barcza Zoltán , Hidy Dóra , Kern Anikó , Dimoski Doroteja , Miko Slobodan , Hasan Ozren , Grahovac Branka , Marjanović Hrvoje
Published: September 2021

Soil organic carbon (SOC) is a mandatory pool in national inventory reports on greenhouse gas (GHG) emissions and removals to the UNFCCC. Hence, its accurate assessment is important. Modelling SOC changes for national GHG reports is encouraged, but the uncertainty related to this pool still presents a significant challenge; thus, verifying modelling results with field observations is essential. We used the process-based model Biome-BGCMuSo and assessed its suitability for use in Croatia’s GHG reporting. We modelled SOC stocks in the top 30 cm of the mineral soil layer (SOC30) for four different land-use (LU) categories (Deciduous/Coniferous Forest, Grassland and Annual Cropland) distributed in three biogeographical regions (Alpine, Continental and Mediterranean) and compared them with results of a national soil survey. A total of 573 plot level simulations were undertaken and results were evaluated at three stratification levels (LU, LU × biogeographical region, and plot). The model reproduced the overall country mean of SOC30 with no overall bias, and showed good performance at the LU level with no significant (p < 0.05) difference for all LUs except Deciduous Forest (11% overestimation). At finer stratifications, the model performance considerably worsened. Further model calibration, improvement and testing, as well as repeated soil survey are needed in order to assess the changes in SOC30 and to evaluate the potential of the Biome-BGCMuSo model for use in GHG reporting.
Counterbalancing the Development Territorial Disparities in the Implementation of the Community-Led Local Development EU Approach in Romania
Authors: Olar Alexandru , Jitea Mugurel I.
Published: September 2021

LEADER is a rural development method based on a participative approach, which was tailored in 1991 as a complement to the traditional common agricultural policy (CAP) measures. One of its most important objectives is to reduce the differences between rural and urban areas by building on local knowledge and potential. The aim of the present paper is to identify what are the most important characteristics of the LAGs that can counterbalance the existing economic disparities in the rural regions. The research was conducted in the northwest development region of Romania (2014–2020 programming period), using the principal component analysis and the hierarchical cluster analysis. Two types of data were collected: indicators of performance, such as the number of projects contracted and jobs created, were used to assess the success of the method, while the territorial and LAG characteristics were used to explain these results. The findings confirm the presence of an unequal distribution of LEADER support in favor of the most urbanized and developed areas. However, the results also show that the experience and economic and administrative capacity of LAGs could help counterbalance the influence of the territorial features previously mentioned, and therefore to reduce the gap between them and the more developed groups.
Fit-for-Purpose Land Administration—Providing Secure Land Rights at Scale
Authors: Enemark Stig , McLaren Robin , Lemmen Christiaan
Published: September 2021

This Special Issue provides an insight, collated from 26 articles, focusing on various aspects of the Fit-for-Purpose Land Administration (FFPLA) concept and its application [...]
Features of the Skin Microbiota in Common Inflammatory Skin Diseases
Authors: Ferček Iva , Lugović-Mihić Liborija , Tambić-Andrašević Arjana , Ćesić Diana , Grginić Ana Gverić , Bešlić Iva , Mravak-Stipetić Marinka , Mihatov-Štefanović Iva , Buntić Ana-Marija , Čivljak Rok
Published: September 2021

Many relatively common chronic inflammatory skin diseases manifest on the face (seborrheic dermatitis, rosacea, acne, perioral/periorificial dermatitis, periocular dermatitis, etc.), thereby significantly impairing patient appearance and quality of life. Given the yet unexplained pathogenesis and numerous factors involved, these diseases often present therapeutic challenges. The term “microbiome” comprises the totality of microorganisms (microbiota), their genomes, and environmental factors in a particular environment. Changes in human skin microbiota composition and/or functionality are believed to trigger immune dysregulation, and consequently an inflammatory response, thereby playing a potentially significant role in the clinical manifestations and treatment of these diseases. Although cultivation methods have traditionally been used in studies of bacterial microbiome species, a large number of bacterial strains cannot be grown in the laboratory. Since standard culture-dependent methods detect fewer than 1% of all bacterial species, a metagenomic approach could be used to detect bacteria that cannot be cultivated. The skin microbiome exhibits spatial distribution associated with the microenvironment (sebaceous, moist, and dry areas). However, although disturbance of the skin microbiome can lead to a number of pathological conditions and diseases, it is still not clear whether skin diseases result from change in the microbiome or cause such a change. Thus far, the skin microbiome has been studied in atopic dermatitis, seborrheic dermatitis, psoriasis, acne, and rosacea. Studies on the possible association between changes in the microbiome and their association with skin diseases have improved the understanding of disease development, diagnostics, and therapeutics. The identification of the bacterial markers associated with particular inflammatory skin diseases would significantly accelerate the diagnostics and reduce treatment costs. Microbiota research and determination could facilitate the identification of potential causes of skin diseases that cannot be detected by simpler methods, thereby contributing to the design and development of more effective therapies.
Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells
Authors: Huang Chun-Yin , Weng Yu-Ting , Li Po-Chen , Hsieh Nien-Tsu , Li Chun-I , Liu Hsiao-Sheng , Lee Ming-Fen
Published: September 2021

Increasing lines of evidence indicate that the biologically active form of vitamin D, calcitriol (1,25-dihydroxyvitamin D3), prevents cancer progression by reducing cell proliferation, increasing cell differentiation, and inhibiting angiogenesis, among other potential roles. Cancer cells in solid tumors preferably undergo the “Warburg effect” to support cell growth by upregulating glycolysis, and the glycolytic intermediates further serve as building blocks to generate biomass. The objective of the current study is to investigate whether calcitriol affects glucose metabolism and cell growth in human colorectal cancer cells. Calcitriol reduced the expression of cyclin D1 and c-Myc. In addition, calcitriol reduced the expression of glucose transporter 1 (GLUT1) and key glycolytic enzymes and decreased extracellular acidification rate but increased oxygen consumption rate in human colorectal cancer cells. In a subcutaneous HT29 xenograft NOD/SCID mouse model, the volume and weight of the tumors were smaller in the calcitriol groups as compared with the control group, and the expression levels of GLUT1 and glycolytic enzymes, hexokinase 2 and lactate dehydrogenase A, were also lower in the calcitriol groups in a dose-responsive manner. Our data indicate that calcitriol suppresses glycolysis and cell growth in human colorectal cancer cells, suggesting an inhibitory role of the biologically active form of vitamin D in colorectal cancer progression.
Exploring the Impact of Terminators on Transgene Expression in Chlamydomonas reinhardtii with a Synthetic Biology Approach
Authors: Geisler Katrin , Scaife Mark A. , Mordaka Paweł M. , Holzer Andre , Tomsett Eleanor V. , Mehrshahi Payam , Mendoza Ochoa Gonzalo I. , Smith Alison G.
Published: September 2021

Chlamydomonas reinhardtii has many attractive features for use as a model organism for both fundamental studies and as a biotechnological platform. Nonetheless, despite the many molecular tools and resources that have been developed, there are challenges for its successful engineering, in particular to obtain reproducible and high levels of transgene expression. Here we describe a synthetic biology approach to screen several hundred independent transformants using standardised parts to explore different parameters that might affect transgene expression. We focused on terminators and, using a standardised workflow and quantitative outputs, tested 9 different elements representing three different size classes of native terminators to determine their ability to support high level expression of a GFP reporter gene. We found that the optimal size reflected the median size of element found in the C. reinhardtii genome. The behaviour of the terminator parts was similar with different promoters, in different host strains and with different transgenes. This approach is applicable to the systematic testing of other genetic elements, facilitating comparison to determine optimal transgene design.
Inhibition of Mild Steel Corrosion by 4-benzyl-1-(4-oxo-4-phenylbutanoyl)thiosemicarbazide: Gravimetrical, Adsorption and Theoretical Studies
Authors: Alamiery Ahmed A. , Wan Isahak Wan Nor Roslam , Takriff Mohd S.
Published: September 2021

Gravimetric measurements were applied to study the inhibitory effect of 4-benzyl-1-(4-oxo-4-phenylbutanoyl)thiosemicarbazide (BOT) on the corrosion of mild steel in 1.0 M HCl. BOT has a good inhibitory efficacy of 92.5 percent at 500 ppm, according to weight loss results. The effect of inhibitor concentration on the mild corrosion behavior of steel was investigated and it was discovered that the higher the inhibitor concentration, the higher the damping efficiency. The results confirm that BOT is an effective corrosion inhibitor for mild steel in the presence of 1.0 M HCl. Furthermore, the higher protection efficiency with increasing temperature and the free energy value showed that BOT molecules participate in both chemisorption (coordination bonds between the active sites of BOT molecules and d-orbital of iron atoms) and physisorption (through the physical interactions on the mild steel surface). The adsorption mechanism on the mild steel surface obeys the Langmuir adsorption isotherm model. Quantum chemical calculations based on the DFT calculations were conducted on BOT. DFT calculations indicated that the protective efficacy of the tested inhibitor increased with the increase in energy of HOMO. The theoretical findings revealed that the broadly stretched linked functional groups (carbonyl and thionyl) and heteroatoms (sulfur, nitrogen and oxygen) in the structure of tested inhibitor molecules are responsible for the significant inhibitive performance, due to possible bonding with Fe atoms on the mild steel surface by donating electrons to the d-orbitals of Fe atoms. Both experimental and theoretical findings in the current investigation are in excellent harmony.
Artificial Intelligence Aided Design of Tissue Engineering Scaffolds Employing Virtual Tomography and 3D Convolutional Neural Networks
Authors: Bermejillo Barrera María Dolores , Franco-Martínez Francisco , Díaz Lantada Andrés
Published: September 2021

Design requirements for different mechanical metamaterials, porous constructions and lattice structures, employed as tissue engineering scaffolds, lead to multi-objective optimizations, due to the complex mechanical features of the biological tissues and structures they should mimic. In some cases, the use of conventional design and simulation methods for designing such tissue engineering scaffolds cannot be applied because of geometrical complexity, manufacturing defects or large aspect ratios leading to numerical mismatches. Artificial intelligence (AI) in general, and machine learning (ML) methods in particular, are already finding applications in tissue engineering and they can prove transformative resources for supporting designers in the field of regenerative medicine. In this study, the use of 3D convolutional neural networks (3D CNNs), trained using digital tomographies obtained from the CAD models, is validated as a powerful resource for predicting the mechanical properties of innovative scaffolds. The presented AI-aided or ML-aided design strategy is believed as an innovative approach in area of tissue engineering scaffolds, and of mechanical metamaterials in general. This strategy may lead to several applications beyond the tissue engineering field, as we analyze in the discussion and future proposals sections of the research study.
Microstructure and Wear Properties of Laser Cladding WC/Ni-Based Composite Layer on Al–Si Alloy
Authors: Wu Xiaoquan , Zhang Daoda , Hu Zhi
Published: September 2021

The microstructural and wear properties of laser-cladding WC/Ni-based layer on Al–Si alloy were investigated by scanning electron microscope (SEM), X-ray diffraction (XRD), energy dispersive spectrometer (EDS) and wear-testing. The results show that, compared with the original specimen, the microhardness and wear resistance of the cladding layer on an Al–Si alloy were remarkably improved, wherein the microhardness of the layer achieved 1100 HV and the average friction coefficient of the layer was barely 0.14. The mainly contributor to such significant improvement was the generation of a WC/Ni-composite layer of Al–Si alloy during laser cladding. Two types of carbides, identified as M7C3 and M23C6, were found in the layer. The wear rate of the layer first increased and then decreased with the increase in load; when the load was 20 N, 60 N and 80 N, the wear rate of layer was1.89 × 10−3 mm3·m−1, 3.73 × 10−3 mm3·m−1 and 2.63 × 10−3 mm3·m−1, respectively, and the average friction coefficient (0.14) was the smallest when the load was 60 N.
Effective Magnetic Field Dependence of the Flux Pinning Energy in FeSe0.5Te0.5 Superconductor
Authors: Khan Masood Rauf , Leo Antonio , Nigro Angela , Galluzzi Armando , Polichetti Massimiliano , Braccini Valeria , Cialone Matteo , Scuderi Mario , Grimaldi Gaia
Published: September 2021

The role of a layered structure in superconducting pinning properties is still at a debate. The effects of the vortex shape, which can assume for example a staircase form, could influence the interplay with extrinsic pinning coming from the specific defects of the material, thus inducing an effective magnetic field dependence. To enlighten this role, we analysed the angular dependence of flux pinning energy U(H,θ) as a function of magnetic field in FeSe0.5Te0.5 thin film by considering the field components along the ab-plane of the crystal structure and the c-axis direction. U(H,θ) has been evaluated from magneto-resistivity measurements acquired at different orientations between the applied field up to 16 T and FeSe0.5Te0.5 thin films grown on a CaF2 substrate. We observed that the U(H,θ) shows an anisotropic trend as a function of both the intensity and the direction of the applied field. Such a behaviour can be correlated to the presence of extended defects elongated in the ab-planes, thus mimicking a layered superconductor, as we observed in the microstructure of the compound. The comparison of FeSe0.5Te0.5 with other superconducting materials provides a more general understanding on the flux pinning energy in layered superconductors.
Torsional Resistance of Heat-Treated Nickel-Titanium Instruments under Different Temperature Conditions
Authors: Jo Hyo Jin , Kwak Sang Won , Kim Hyeon-Cheol , Kim Sung Kyo , Ha Jung-Hong
Published: September 2021

This study compared the torsional resistance of heat-treated nickel-titanium (NiTi) instruments under different temperature conditions. Four thermomechanically treated single-use NiTi rotary instruments were selected for this study: OneShape (OS), OneCurve (OC), WaveOne Gold (WOG) and HyFlex EDM (HFE). Each instrument was further subdivided by temperature into 2 subgroups. Maximum torque and the distortion angle until fracture occurred were evaluated. Differential scanning calorimetry analysis was performed to measure the phase transformation temperature. Statistical analysis was performed using a two-way ANOVA and t-test (p < 0.05). Fractured fragments were observed using scanning electron microscopy (SEM). The two-way ANOVA showed no significant differences for different temperature conditions. At both room (RT) and body temperature (BT), OS was predominantly austenite while HFE was martensite. OC and WOG were predominantly martensite at RT and mixed phase at BT. At BT, more than half of WOG was martensite, while half of OC was austenite. SEM examination showed no topographical differences between instruments in different temperature groups. In relation to a limitation of this study, there was no difference in torsional resistance of NiTi rotary instruments between the BT and RT conditions. This implies that clinicians do not need to consider a decrease of torsional resistance of heat-treated NiTi instruments at BT.
Innovative Biochar-Based Composite Fibres from Recycled Material
Authors: Lepak-Kuc Sandra , Kiciński Mateusz , Michalski Przemyslaw P. , Pavlov Krystian , Giorcelli Mauro , Bartoli Mattia , Jakubowska Malgorzata
Published: September 2021

Carbon materials are becoming crucial in several industrial sectors. The drawbacks of these materials include their high cost and oil-based essence. In recent years, recycled materials have become possible alternative sources of carbon with several advantages. Firstly, the production of this alternative source of carbon may help to reduce biomass disposal, and secondly, it contributes to CO2 sequestration. The use of carbon derived from recycled materials by a pyrolysis treatment is called biochar. Here, we present composite materials based on different biochar filler contents dispersed in several thermoplastic polymer matrixes. Electrical conductivity and tensile break strength were investigated together with the material characterisation by DTA/TGA, XRD, and scanning electron microscopy (SEM) imaging. Materials with good flexibility and electrical conductivity were obtained. The local ordering in composites resembles both biochar and polymer ordering. The similarity between biochar and carbon nanotubes’ (CNTs) XRD patterns may be observed. As biochar is highly cost-effective, the proposed composites could become a valid substitute for CNT composites in various applications.
Functionality of Silk Cocoon (Bombyx mori L.) Sericin Extracts Obtained through High-Temperature Hydrothermal Method
Authors: Wang Wei-Hsun , Lin Wen-Shin , Shih Chia-Hung , Chen Cheng-You , Kuo Siao-Hong , Li Wei-Lin , Lin Yung-Sheng
Published: September 2021

Sericin, a textile waste, can be used for antioxidant and skin-whitening purposes. The hydrothermal method of extracting sericin is more eco-friendly than are chemical and enzymatic methods. In this study, silk cocoons were cut into pieces and then subjected to hydrothermal extraction at three temperatures (160, 200, and 220 °C) to obtain sericin extracts (Sericin160, Sericin200, and Sericin220, respectively). Antioxidant activity and tyrosinase inhibition were measured to determine the extracts’ effectiveness. Sericin220 was the strongest antioxidant, with total phenol content, total flavonoid content, and ferric reducing power of 62.19 ± 0.04 mg gallic acid equivalents/g dry weight, 0.07 ± 0.01 mg quercetin equivalent/g dry weight, and 181.49 ± 0.024 mg vitamin C equivalent/g dry weight, respectively. The half-maximal inhibitory concentrations for DPPH and ABTS free-radical scavenging ability were 6.41 ± 0.05 and 0.79 ± 0.37 mg/mL, respectively. Sericin220 also exhibited the highest tyrosinase inhibition activity (70.82 ± 4.1 mg vitamin C equivalent/g), indicating its whitening potential.
Internal Crack Initiation and Growth Starting from Artificially Generated Defects in Additively Manufactured Ti6Al4V Specimen in the VHCF Regime
Authors: Wickmann Carsten , Benz Christopher , Heyer Horst , Witte-Bodnar Kerstin , Schäfer Jan , Sander Manuela
Published: September 2021

The aim of the present work was to investigate the ‘fine granular area’ (FGA) formation based on artificially generated internal defects in additively manufactured Ti6Al4V specimens in the early stage of fatigue crack growth in the ‘very high cycle fatigue’ (VHCF) regime. Fatigue tests were performed with constant amplitude at pure tension-compression loading (R = −1) using an ultrasonic fatigue testing setup. Failed specimens were investigated using optical microscopy, high-resolution ‘scanning electron microscopy’ (SEM), and ‘focused ion beam’ (FIB) techniques. Further, the paper introduces alternative proposals to identify the FGA layer beneath the fracture surfaces in terms of the ‘cross section polishing’ (CSP) technique and metallic grindings with special attention paid to the crack origin, the surrounding microstructure, and the expansion of the nanograin layer beneath the fracture surface. Different existing fracture mechanical approaches were applied to evaluate if an FGA formation is possible. Moreover, the results were discussed in comparison to the experimental findings.
Multiple Porous Synthetic Bone Graft Comprising EngineeredMicro-Channel for Drug Carrier and Bone Regeneration
Authors: Bae Chun-Sik , Kim Seung-Hyun , Ahn Taeho , Kim Yeonji , Kim Se-Eun , Kang Seong-Soo , Kwon Jae-Sung , Kim Kwang-Mahn , Kim Sahng-Gyoon , Oh Daniel
Published: September 2021

Due to high demand but limited supply, there has been an increase in the need to replace autologous bone grafts with alternatives that fulfill osteogenic requirements. In this study, two different types of bone grafts were tested for their drug carrying abilities along with their osteogenic properties. Two different types of alendronate-loaded bone grafts, Bio-Oss (bovine bone graft) and InRoad (biphasic synthetic bone graft) were observed to see how different concentrations of alendronate would affect the sustained release to enhance osteogenesis. In this study, defected ovariectomize-induced osteoporotic rat calvarias were observed for 28 days with three different concentrations of alendronate (0 mg, 1 mg, 5 mg) for both Bio-Oss and InRoad. A higher concentration (5 mg) allowed for a more controlled and sustained release throughout the 28-day comparison to those of lower concentrations (0 mg, 1 mg). When comparing Bio-Oss and InRoad through histology and Micro-CT, InRoad showed higher enhancement in osteogenesis. Through this study, it was observed that alendronate not only brings out robust osteogenesis with InRoad bone grafts, but also enhances bone regeneration in an alendronate-concentration-dependent manner. The combination of higher concentration of alendronate and multiple porous bone graft containing internal micro-channel structure of InRoad resulted in higher osteogenesis with a sustained release of alendronate.
2D CNN-Based Multi-Output Diagnosis for Compound Bearing Faults under Variable Rotational Speeds
Authors: Pham Minh-Tuan , Kim Jong-Myon , Kim Cheol-Hong
Published: September 2021

Bearings prevent damage caused by frictional forces between parts supporting the rotation and they keep rotating shafts in their correct position. However, the continuity of work under harsh conditions leads to inevitable bearing failure. Thus, methods for bearing fault diagnosis (FD) that can predict and categorize fault type, as well as the level of degradation, are increasingly necessary for factories. Owing to the advent of deep neural networks, especially convolutional neural networks (CNNs), intelligent FD methods have achieved significantly higher performance in terms of accuracy. However, in addition to accuracy, the efficiency issue still needs to be weathered in complicated diagnosis scenarios to adapt to real industrial environments. Here, we introduce a method based on multi-output classification, which utilizes the correlated features extracted for bearing compound fault type classification and crack-size classification to serve both aims. Additionally, the synergy of a time–frequency signal processing method and the proposed two-dimensional CNN helped the method perform well under the condition of variable rotational speeds. Monitoring signals of acoustic emission also had advantages for incipient FD. The experimental results indicated that utilizing correlated features in multi-output classification improved both the accuracy and efficiency of multi-task diagnosis compared to conventional CNN-based multiclass classification.
The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles
Authors: Bobrikova Ekaterina , Chubarov Alexey , Dmitrienko Elena
Published: September 2021

Magnetic Fe3O4 nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe3O4 MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.
Mathematical Principles of Object 3D Reconstruction by Shape-from-Focus Methods
Authors: Martišek Dalibor , Mikulášek Karel
Published: September 2021

Shape-from-Focus (SFF) methods have been developed for about twenty years. They able to obtain the shape of 3D objects from a series of partially focused images. The plane to which the microscope or camera is focused intersects the 3D object in a contour line. Due to wave properties of light and due to finite resolution of the output device, the image can be considered as sharp not only on this contour line, but also in a certain interval of height—the zone of sharpness. SSFs are able to identify these focused parts to compose a fully focused 2D image and to reconstruct a 3D profile of the surface to be observed.
“Go Wild for a While!”: A New Test for Forecast Evaluation in Nested Models
Authors: Pincheira Pablo , Hardy Nicolás , Muñoz Felipe
Published: September 2021

In this paper, we present a new asymptotically normal test for out-of-sample evaluation in nested models. Our approach is a simple modification of a traditional encompassing test that is commonly known as Clark and West test (CW). The key point of our strategy is to introduce an independent random variable that prevents the traditional CW test from becoming degenerate under the null hypothesis of equal predictive ability. Using the approach developed by West (1996), we show that in our test, the impact of parameter estimation uncertainty vanishes asymptotically. Using a variety of Monte Carlo simulations in iterated multi-step-ahead forecasts, we evaluated our test and CW in terms of size and power. These simulations reveal that our approach is reasonably well-sized, even at long horizons when CW may present severe size distortions. In terms of power, results were mixed but CW has an edge over our approach. Finally, we illustrate the use of our test with an empirical application in the context of the commodity currencies literature.
Algorithm Execution Time and Accuracy of NTC Thermistor-Based Temperature Measurements in Time-Critical Applications
Authors: Petkovšek Marko , Nemec Mitja , Zajec Peter
Published: September 2021

This paper addresses the challenges of selecting a suitable method for negative temperature coefficient (NTC) thermistor-based temperature measurement in electronic devices. Although measurement accuracy is of great importance, the temperature calculation time represents an even greater challenge since it is inherently constrained by the control algorithm executed in the microcontroller (MCU). Firstly, a simple signal conditioning circuit with the NTC thermistor is introduced, resulting in a temperature-dependent voltage UT being connected to the MCU’s analog input. Next, a simulation-based approximation of the actual temperature vs. voltage curve is derived, resulting in four temperature notations: for a look-up table principle, polynomial approximation, B equation and Steinhart–Hart equation. Within the simulation results, the expected temperature error of individual methods is calculated, whereas in the experimental part, performed on a DC/DC converter prototype, required prework and available MCU resources are evaluated. In terms of expected accuracy, the look-up table and the Steinhart–Hart equation offer superior results over the polynomial approximation and B equation, especially in the nominal temperature range of the NTC thermistor. However, in terms of required prework, the look-up table is inferior compared to the Steinhart–Hart equation, despite the latter having far more complex mathematical functions, affecting the overall MCU algorithm execution time significantly.
Guidance Level for Brevetoxins in French Shellfish
Authors: Arnich Nathalie , Abadie Eric , Amzil Zouher , Dechraoui Bottein Marie-Yasmine , Comte Katia , Chaix Estelle , Delcourt Nicolas , Hort Vincent , Mattei César , Molgó Jordi , Le Garrec Raphaele
Published: September 2021

Brevetoxins (BTXs) are marine biotoxins responsible for neurotoxic shellfish poisoning (NSP) after ingestion of contaminated shellfish. NSP is characterized by neurological, gastrointestinal and/or cardiovascular symptoms. The main known producer of BTXs is the dinoflagellate Karenia brevis, but other microalgae are also suspected to synthesize BTX-like compounds. BTXs are currently not regulated in France and in Europe. In November 2018, they have been detected for the first time in France in mussels from a lagoon in the Corsica Island (Mediterranean Sea), as part of the network for monitoring the emergence of marine biotoxins in shellfish. To prevent health risks associated with the consumption of shellfish contaminated with BTXs in France, a working group was set up by the French Agency for Food, Environmental and Occupational Health & Safety (Anses). One of the aims of this working group was to propose a guidance level for the presence of BTXs in shellfish. Toxicological data were too limited to derive an acute oral reference dose (ARfD). Based on human case reports, we identified two lowest-observed-adverse-effect levels (LOAELs). A guidance level of 180 µg BTX-3 eq./kg shellfish meat is proposed, considering a protective default portion size of 400 g shellfish meat.
Treatment Modalities of Recurrent Oral Mucosal Melanoma In Situ
Authors: Becker Philipp , Pabst Andreas , Bjelopavlovic Monika , Müller Daniel , Kämmerer Peer W.
Published: September 2021

Oral mucosal melanoma (OMM) represents an extremely rare entity that is associated with a poor prognosis due to late diagnosis and early metastasis. Here, extensive surgical therapy is the therapy of choice. In contrary, for OMM in situ, the respective therapeutical recommendations are lacking. In this case report, treatment modalities of an OMM in situ of the palate, including the maxillary alveolar process, are reported. The tumor relapsed twice despite adequate surgical therapy and reconstruction. Therefore, irradiation was performed as an adjuvant therapy. At a follow-up of two years, the patient was free from recurrences.
Ethics in Medicines: Exposing Unethical Practices and Corruption in All Sectors of Medicines Is Essential for Improving Global Public Health and Saving Patients’ Lives
Authors: Kontoghiorghes George J.
Published: September 2021

While great strides have been made in science and medicine ensuring better living standards and health care for most human beings, many issues still remain, which are responsible for an increase in mortality and morbidity rates of millions of people worldwide, despite that in most cases the causes are preventable [...]
Sorption of Heavy Metals by Sewage Sludge and Its Mixtures with Soil from Wastewater Treatment Plants Operating in MBR and INR Technology
Authors: Kowalik Robert , Widłak Małgorzata , Widłak Agata
Published: September 2021

Sewage sludge is a very complex system, with solids and water. It is generated as waste from wastewater treatment. Sewage sludge is used to fertilize agricultural and forest areas and to rehabilitate devastated areas. It is a good organic fertilizer because it contains significant amounts of nutrients beneficial for plant development and humus-forming substances. The composition of sludge from municipal wastewater treatment plants is similar to soil organic matter, therefore it can be used to improve the physicochemical properties of soil, increasing its sorption capacity. Research material was collected in the Swietokrzyskie and Mazowieckie Voivodships. Sewage sludge was collected from the wastewater treatment plants in Sitkowka Nowiny (Sitkowka) and Kunow, as well as high-quality agricultural soil from Opatowiec and sandy-clay soil from Jastrzebie. Research was carried out on the sorption of heavy metals (Cd, Cr, Cu, Pb, Ni, Zn) by mixtures of sewage sludge with soil. The calculations were made for the concentrations of heavy metals in sewage sludge, soil, and sewage sludge–soil mixtures. The geoaccumulation index (Igeo) and the risk assessment code (RAC) were calculated. Increased sorption capacity was demonstrated in samples with a predominance of sewage sludge. It was shown that heavy metals from sewage sludge, after mixing with soil, changed their form from immobile to mobile.
Influence of Membrane Vibration on Particles Rejection Using a Slotted Pore Membrane Microfiltration
Authors: Ullah Asmat , Alam Kamran , Khan Saad Ullah , Starov Victor M.
Published: September 2021

A new method is proposed to increase the rejection in microfiltration by applying membrane oscillation, using a new type of microfiltration membrane with slotted pores. The oscillations applied to the membrane surface result in reduced membrane fouling and increased separation efficiency. An exact mathematical solution of the flow in the surrounding solution outside the oscillating membrane is developed. The oscillation results in the appearance of a lift velocity, which moves oil particles away from the membrane. The latter results in both reduced membrane fouling and increased oil droplet rejection. This developed model was supported by the experimental results for oil water separation in the produced water treatment. It was proven that the oil droplet concentration was reduced notably in the permeate, due to the membrane oscillation, and that the applied shear rate caused by the membrane oscillation also reduced pore blockage. A four-times lower oil concentration was recorded in the permeate when the membrane vibration frequency was 25 Hz, compared to without membrane vibration. Newly generated microfiltration membranes with slotted pores were used in the experiments.
Comparative In Vivo Analysis of the Integration Behavior and Immune Response of Collagen-Based Dental Barrier Membranes for Guided Bone Regeneration (GBR)
Authors: Radenković Milena , Alkildani Said , Stoewe Ignacio , Bielenstein James , Sundag Bernd , Bellmann Olaf , Jung Ole , Najman Stevo , Stojanović Sanja , Barbeck Mike
Published: September 2021

Collagen-based resorbable barrier membranes have been increasingly utilized for Guided Bone Regeneration (GBR), as an alternative to non-resorbable synthetic membranes that require a second surgical intervention for removal. One of the most important characteristics of a resorbable barrier membrane is its mechanical integrity that is required for space maintenance and its tissue integration that plays a crucial role in wound healing and bone augmentation. This study compares a commercially available porcine-derived sugar-crosslinked collagen membrane with two non-crosslinked collagen barrier membranes. The material analysis provides an insight into the influence of manufacturing on the microstructure. In vivo subcutaneous implantation model provides further information on the host tissue reaction of the barrier membranes, as well as their tissue integration patterns that involve cellular infiltration, vascularization, and degradation. The obtained histochemical and immunohistochemical results over three time points (10, 30, and 60 days) showed that the tissue response to the sugar crosslinked collagen membrane involves inflammatory macrophages in a comparable manner to the macrophages observed in the surrounding tissue of the control collagen-based membranes, which were proven as biocompatible. The tissue reactions to the barrier membranes were additionally compared to wounds from a sham operation. Results suggest wound healing properties of all the investigated barrier membranes. However, the sugar-crosslinked membrane lacked in cellular infiltration and transmembraneous vascularization, providing an exclusive barrier function in GBR. Moreover, this membrane maintained a similar swelling ratio over examined timepoints, which suggests a very slow degradation pattern and supports its barrier function. Based on the study results, which showed biocompatibility of the sugar crosslinked membrane and its stability up to 60 days post-implantation, it can be concluded that this membrane may be suitable for application in GBR as a biomaterial with exclusive barrier functionality, similar to non-resorbable options.
Microstructure and Mechanical Properties of Composites Obtained by Spark Plasma Sintering of Al–Fe66Cr10Nb5B19 Metallic Glass Powder Mixtures
Authors: Dudina Dina V. , Bokhonov Boris B. , Batraev Igor S. , Kvashnin Vyacheslav I. , Legan Mikhail A. , Novoselov Aleksey N. , Anisimov Alexander G. , Esikov Maksim A. , Ukhina Arina V. , Matvienko Alexander A. , Georgarakis Konstantinos , Koga Guilherme Yuuki , Moreira Jorge Alberto
Published: September 2021

At present, metallic glasses are evaluated as alternative reinforcements for aluminum matrix composites. These composites are produced by powder metallurgy via consolidation of metallic glass-aluminum powder mixtures. In most studies, the goal has been to preserve the glassy state of the reinforcement during consolidation. However, it is also of interest to track the structure evolution of these composites when partial interaction between the matrix and the metallic glass is allowed during sintering of the mixtures. The present work was aimed to study the microstructure and mechanical properties of composites obtained by spark plasma sintering (SPS) of Al-20 vol.% Fe66Cr10Nb5B19 metallic glass mixtures and compare the materials, in which no significant interaction between the matrix and the Fe-based alloy occurred, with those featuring reaction product layers of different thicknesses. Composite materials were consolidated by SPS at 540 and 570 °C. The microstructure and mechanical properties of composites obtained by SPS and SPS followed by forging, composites with layers of interfacial reaction products of different thicknesses, and metallic glass-free sintered aluminum were comparatively analyzed to conclude on the influence of the microstructural features of the composites on their strength.
Kinetic Aspects of Phosphorus Removal from Electromagnetically Levitated 600 MPa Steel Droplets
Authors: Jiang Qi , Zhang Guifang , Yang Yindong , McLean Alexander , Gao Lei
Published: September 2021

A kinetic model was developed to study the dephosphorization of 600 MPa steel droplets under electromagnetic levitation conditions. The relationships which were derived from the model between dephosphorization and the influence of temperature and gas flow rate were in good agreement with experimental data. Both temperature and gas flow rate were conducive to the evaporation of phosphorus, with the effect of temperature having a greater influence than that of the gas velocity. The results show that the rate-controlling step for the dephosphorization process was diffusion within the gas phase. This work aims to provide a theoretical basis for process optimization during the dephosphorization of 600 MPa steel.
Titanium Alloys and Titanium-Based Matrix Composites
Authors: Motyka Maciej
Published: September 2021

Titanium alloys have been considered unique materials for many years [...]
Comparative Untargeted Metabolomic Profiling of Induced Mitochondrial Fusion in Pancreatic Cancer
Authors: Nguyen Nicholas D. , Yu Meifang , Reddy Vinit Y. , Acevedo-Diaz Ariana C. , Mesarick Enzo C. , Abi Jaoude Joseph , Yuan Min , Asara John M. , Taniguchi Cullen M.
Published: September 2021

Mitochondria are dynamic organelles that constantly alter their shape through the recruitment of specialized proteins, like mitofusin-2 (Mfn2) and dynamin-related protein 1 (Drp1). Mfn2 induces the fusion of nearby mitochondria, while Drp1 mediates mitochondrial fission. We previously found that the genetic or pharmacological activation of mitochondrial fusion was tumor suppressive against pancreatic ductal adenocarcinoma (PDAC) in several model systems. The mechanisms of how these different inducers of mitochondrial fusion reduce pancreatic cancer growth are still unknown. Here, we characterized and compared the metabolic reprogramming of these three independent methods of inducing mitochondrial fusion in KPC cells: overexpression of Mfn2, genetic editing of Drp1, or treatment with leflunomide. We identified significantly altered metabolites via robust, orthogonal statistical analyses and found that mitochondrial fusion consistently produces alterations in the metabolism of amino acids. Our unbiased methodology revealed that metabolic perturbations were similar across all these methods of inducing mitochondrial fusion, proposing a common pathway for metabolic targeting with other drugs.