bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Agro-Ecological Apple Replant Disease (ARD) Management Strategies: Organic Fertilisation and Inoculation with Mycorrhizal Fungi and Bacteria
Authors: Cavael Ulrike , Lentzsch Peter , Schwärzel Hilmar , Eulenstein Frank , Tauschke Marion , Diehl Katharina
Published: February 2021

Apple replant disease (ARD) impacts the economic yield of orchards by physiological and morphological suppression of apple trees on replanted soils. The complexity of replant disease caused by a plethora of biological interactions and physical properties of the soil requires complex management strategies to mitigate these effects. Based on expert recommendations, we selected two management strategies linked to agroecological principles of (a) organic fertilisation with a specific mulch composition (MDK) and (b) biofertilisation with arbuscular mycorrhizal and bacterial strains (AMFbac), applied by a composition of existing products. For both management strategies we provide a proof-of-concept, by pot and field experiments. Both treatments have the potential to mitigate ARD effects on plant vigour. ARD effect was fully mitigated by MDK treatment in the short-term (one year) and was mitigated by up to 29% after seven years of MDK treatment (long-term). MDK provides an additional substrate for root growth. AMFbac has the potential to mitigate ARD effects on plant vigour but with non-replicable plant-beneficial effects in its current form of application. Thereby our results show a principal potential to mitigate economic effects but not to overcome replant disease inducing effects. While the MDK treatment is found resource intensive but reliable, the AMFbac treatment was found more user-friendly.
Challenges and Tendencies of Automatic Milking Systems (AMS): A 20-Years Systematic Review of Literature and Patents
Authors: Cogato Alessia , Brščić Marta , Guo Hao , Marinello Francesco , Pezzuolo Andrea
Published: February 2021

Over the last two decades, the dairy industry has adopted the use of Automatic Milking Systems (AMS). AMS have the potential to increase the effectiveness of the milking process and sustain animal welfare. This study assessed the state of the art of research activities on AMS through a systematic review of scientific and industrial research. The papers and patents of the last 20 years (2000–2019) were analysed to assess the research tendencies. The words appearing in title, abstract and keywords of a total of 802 documents were processed with the text mining tool. Four clusters were identified (Components, Technology, Process and Animal). For each cluster, the words frequency analysis enabled us to identify the research tendencies and gaps. The results showed that focuses of the scientific and industrial research areas complementary, with scientific papers mainly dealing with topics related to animal and process, and patents giving priority to technology and components. Both scientific and industrial research converged on some crucial objectives, such as animal welfare, process sustainability and technological development. Despite the increasing interest in animal welfare, this review highlighted that further progress is needed to meet the consumers’ demand. Moreover, milk yield is still regarded as more valuable compared to milk quality. Therefore, additional effort is necessary on the latter. At the process level, some gaps have been found related to cleaning operations, necessary to improve milk quality and animal health. The use of farm data and their incorporation on herd decision support systems (DSS) appeared optimal. The results presented in this review may be used as an overall assessment useful to address future research.
Detection of Serum Allergen-Specific IgE in Atopic Dogs Tested in Northern Italy: Preliminary Study
Authors: Di Tommaso Morena , Luciani Alessia , Crisi Paolo Emidio , Beschi Marica , Rosi Paolo , Rocconi Francesca , Miglio Arianna
Published: February 2021

Canine atopic dermatitis (CAD) is a pruritic allergic skin disease associated with IgE-mediated hypersensitivity. IgE is detected using Serum Allergen-Specific IgE test (SAT) in order to identify allergens. The present study aims to identify the environmental allergens in atopic dogs living in Northern Italy using SAT. The screening SAT (sSAT), using a monoclonal antibody cocktail-based ELISA to identify indoor and outdoor allergens, was performed. In all positive samples, an anti-IgE monoclonal antibody ELISA test was performed to extend panel of allergens. Out of 117 selected dogs, 69 were included in the study; 71% were positive and 29% were negative to sSAT. Among the 49 positive sSAT, 53% were positive for both indoor and outdoor, 38.8% only for indoor, and 8.2% only for outdoor allergens. This is the first study on the frequency of allergens involved in CAD in Italy using SAT. IgE hypersensitivity in atopic dogs of Northern Italy is usually associated with indoor allergens, primarily house dust mites. Among the outdoor allergens, an important role was played by Rumex acetosa. Polysensitization also commonly occurs. Therefore, since the numerous factors affect the IgE positivity in CAD, specific panels for geographical areas should be considered and re-evaluated at time intervals.
Effects of Essential Oils-Based Supplement and Salmonella Infection on Gene Expression, Blood Parameters, Cecal Microbiome, and Egg Production in Laying Hens
Authors: Laptev Georgi Yu. , Yildirim Elena A. , Ilina Larisa A. , Filippova Valentina A. , Kochish Ivan I. , Gorfunkel Elena P. , Dubrovin Andrei V. , Brazhnik Evgeni A. , Narushin Valeriy G. , Novikova Natalia I. , Novikova Oksana B. , Dunyashev Timur P. , Smolensky Vladimir I. , Surai Peter F. , Griffin Darren K. , Romanov Michael N.
Published: February 2021

One of the main roles in poultry resistance to infections caused by Salmonella is attributed to host immunity and intestinal microbiota. We conducted an experiment that involved challenging Lohmann White laying hens with Salmonella Enteritidis (SE), feeding them a diet supplemented with an EOs-based phytobiotic Intebio®. At 1 and 7 days post-inoculation, the expression profiles of eight genes related to immunity, transport of nutrients in the intestine, and metabolism were examined. Cecal microbiome composition and blood biochemical/immunological indices were also explored and egg production traits recorded. As a result, the SE challenge of laying hens and Intebio® administration had either a suppressive or activating effect on the expression level of the studied genes (e.g., IL6 and BPIFB3), the latter echoing mammalian/human tissue-specific expression. There were also effects of the pathogen challenge and phytobiotic intake on the cecal microbiome profiles and blood biochemical/immunological parameters, including those reflecting the activity of the birds’ immune systems (e.g., serum bactericidal activity, β-lysine content, and immunoglobulin levels). Significant differences between control and experimental subgroups in egg performance traits (i.e., egg weight/number/mass) were also found. The phytobiotic administration suggested a positive effect on the welfare and productivity of poultry.
Overfeeding Extends the Period of Annual Cyclicity but Increases the Risk of Early Embryonic Death in Shetland Pony Mares
Authors: D’Fonseca Nicky M. M. , Gibson Charlotte M. E. , Hummel Iris , van Doorn David A. , Roelfsema Ellen , Stout Tom A. E. , van den Broek Jan , de Ruijter-Villani Marta
Published: February 2021

Obesity has been associated with altered reproductive activity in mares, and may negatively affect fertility. To examine the influence of long-term high-energy (HE) feeding on fertility, Shetland pony mares were fed a diet containing 200% of net energy (NE) requirements during a three-year study. The incidence of hemorrhagic anovulatory follicles (HAF) and annual duration of cyclicity were compared to those in control mares receiving a maintenance diet. Day-7 embryos were flushed and transferred between donor and recipient mares from both groups; the resulting conceptuses were collected 21 days after transfer to assess conceptus development. HE mares became obese, and embryos recovered from HE mares were more likely to succumb to early embryonic death. The period of annual cyclicity was extended in HE compared to control mares in all years. The incidence of HAFs did not consistently differ between HE and control mares. No differences in embryo morphometric parameters were apparent. In conclusion, consuming a HE diet extended the duration of cyclicity, and appeared to increase the likelihood of embryos undergoing early embryonic death following embryo transfer.
Novel Anti-FOLR1 Antibody–Drug Conjugate MORAb-202 in Breast Cancer and Non-Small Cell Lung Cancer Cells
Authors: Matsunaga Yuki , Yamaoka Toshimitsu , Ohba Motoi , Miura Sakiko , Masuda Hiroko , Sangai Takafumi , Takimoto Masafumi , Nakamura Seigo , Tsurutani Junji
Published: February 2021

Antibody–drug conjugates (ADCs), which are currently being developed, may become promising cancer therapeutics. Folate receptor α (FOLR1), a glycosylphosphatidylinositol-anchored membrane protein, is an attractive target of ADCs, as it is largely absent from normal tissues but is overexpressed in malignant tumors of epithelial origin, including ovarian, lung, and breast cancer. In this study, we tested the effects of novel anti-FOLR1 antibody–eribulin conjugate MORAb-202 in breast cancer and non-small cell lung cancer (NSCLC) cell lines. FOLR1 expression, cell proliferation, bystander killing effects, and apoptosis were evaluated in seven breast cancer and nine NSCLC cell lines treated with MORAb-202. Tumor growth and FOLR1 expression were assessed in T47D and MCF7 orthotopic xenograft mouse models after a single intravenous administration of MORAb-202 (5 mg/kg). MORAb-202 was associated with inhibited cell proliferation, with specific selectivity toward FOLR1-expressing breast cancer cell lines. Eribulin, the payload of MORAb-202, was unleashed in HCC1954 cells, diffused into intercellular spaces, and then killed the non-FOLR1-expressing MCF7 cells in co-culture systems. In orthotopic xenograft mouse models, FOLR1-expressing T47D tumors and non-FOLR1-expressing MCF7 tumors were suppressed upon MORAb-202 administration. The novel anti-FOLR1 antibody–eribulin conjugate MORAb-202 has potential antitumor effects in breast cancer.
Novel Targets and Mechanisms in Antimicrobial Drug Discovery
Authors: Tondi Donatella
Published: February 2021

The spread of infections resistant to available anti-infective drugs is a serious menace to human health [...]
Tyrosine Phosphorylation Modulates Peroxiredoxin-2 Activity in Normal and Diseased Red Cells
Authors: Mattè Alessandro , Federti Enrica , Tibaldi Elena , Di Paolo Maria Luisa , Bisello Giovanni , Bertoldi Mariarita , Carpentieri Andrea , Pucci Pietro , Iatcencko Iana , Wilson Anand B. , Riccardi Veronica , Siciliano Angela , Turrini Francesco , Kim Dae Won , Choi Soo Young , Brunati Anna Maria , De Franceschi Lucia
Published: February 2021

Peroxiredoxin-2 (Prx2) is the third most abundant cytoplasmic protein in red blood cells. Prx2 belongs to a well-known family of antioxidants, the peroxiredoxins (Prxs), that are widely expressed in mammalian cells. Prx2 is a typical, homodimeric, 2-Cys Prx that uses two cysteine residues to accomplish the task of detoxifying a vast range of organic peroxides, H2O2, and peroxynitrite. Although progress has been made on functional characterization of Prx2, much still remains to be investigated on Prx2 post-translational changes. Here, we first show that Prx2 is Tyrosine (Tyr) phosphorylated by Syk in red cells exposed to oxidation induced by diamide. We identified Tyr-193 in both recombinant Prx2 and native Prx2 from red cells as a specific target of Syk. Bioinformatic analysis suggests that phosphorylation of Tyr-193 allows Prx2 conformational change that is more favorable for its peroxidase activity. Indeed, Syk-induced Tyr phosphorylation of Prx2 enhances in vitro Prx2 activity, but also contributes to Prx2 translocation to the membrane of red cells exposed to diamide. The biologic importance of Tyr-193 phospho-Prx2 is further supported by data on red cells from a mouse model of humanized sickle cell disease (SCD). SCD is globally distributed, hereditary red cell disorder, characterized by severe red cell oxidation due to the pathologic sickle hemoglobin. SCD red cells show Tyr-phosphorylated Prx2 bound to the membrane and increased Prx2 activity when compared to healthy erythrocytes. Collectively, our data highlight the novel link between redox related signaling and Prx2 function in normal and diseased red cells.
Melatonin and Cancer: A Polyhedral Network Where the Source Matters
Authors: Bonmati-Carrion Maria-Angeles , Tomas-Loba Antonia
Published: February 2021

Melatonin is one of the most phylogenetically conserved signals in biology. Although its original function was probably related to its antioxidant capacity, this indoleamine has been “adopted” by multicellular organisms as the “darkness signal” when secreted in a circadian manner and is acutely suppressed by light at night by the pineal gland. However, melatonin is also produced by other tissues, which constitute its extrapineal sources. Apart from its undisputed chronobiotic function, melatonin exerts antioxidant, immunomodulatory, pro-apoptotic, antiproliferative, and anti-angiogenic effects, with all these properties making it a powerful antitumor agent. Indeed, this activity has been demonstrated to be mediated by interfering with various cancer hallmarks, and different epidemiological studies have also linked light at night (melatonin suppression) with a higher incidence of different types of cancer. In 2007, the World Health Organization classified night shift work as a probable carcinogen due to circadian disruption, where melatonin plays a central role. Our aim is to review, from a global perspective, the role of melatonin both from pineal and extrapineal origin, as well as their possible interplay, as an intrinsic factor in the incidence, development, and progression of cancer. Particular emphasis will be placed not only on those mechanisms related to melatonin’s antioxidant nature but also on the recently described novel roles of melatonin in microbiota and epigenetic regulation.
The Influence of Flaxseed Oil Cake Extract on Oxidative Stability of Microencapsulated Flaxseed Oil in Spray-Dried Powders
Authors: Drozłowska Emilia , Bartkowiak Artur , Trocer Paulina , Kostek Mateusz , Tarnowiecka-Kuca Alicja , Bienkiewicz Grzegorz , Łopusiewicz Łukasz
Published: February 2021

The objective of the study was to investigate the application of flaxseed oil cake extract (FOCE) for oxidative stabilization of flaxseed oil in spray-dried emulsions. Two variants of powders with 10% and 20% of flaxseed oil (FO), FOCE, and wall material (maltodextrin and starch Capsul®) were produced by spray-drying process at 180 °C. The oxidative stability of FO was monitored during four weeks of storage at 4 °C by peroxide value (PV) and thiobarbituric acid-reactive substances (TBARS) measurements. Additionally, the fatty acids content (especially changes in α-linolenic acid content), radical scavenging activity, total polyphenolics content, color changes and free amino acids content were evaluated. Obtained results indicated that FOCE could be an adequate antioxidant dedicated for spray-dried emulsions, especially with a high content of FO (20%). These results have important implications for the flaxseed oil encapsulation with natural antioxidant agents obtained from plant-based agro-industrial by product, meeting the goals of circular economy and the idea of zero waste.
Val16A SOD2 Polymorphism Promotes Epithelial–Mesenchymal Transition Antagonized by Muscadine Grape Skin Extract in Prostate Cancer Cells
Authors: Sweeney Janae D. , Debeljak Marija , Riel Stacy , Millena Ana Cecilia , Eshleman James R. , Paller Channing J. , Odero-Marah Valerie
Published: February 2021

Epithelial–mesenchymal transition (EMT), a key event in cancer metastasis, allows polarized epithelial cells to assume mesenchymal morphologies, enhancing invasiveness and migration, and can be induced by reactive oxygen species (ROS). Val16A (Ala) SOD2 polymorphism has been associated with increased prostate cancer (PCa) risk. We hypothesized that SOD2 Ala single nucleotide polymorphism (SNP) may promote EMT. We analyzed SOD2 expression and genotype in various prostate cell lines. Stable overexpression of Ala-SOD2 or Val-SOD2 allele was performed in Lymph Node Carcinoma of the Prostate (LNCaP) cells followed by analysis of intracellular ROS and EMT marker protein expression. Treatments were performed with muscadine grape skin extract (MSKE) antioxidant, with or without addition of H2O2 to provide further oxidative stress. Furthermore, MTS cell proliferation, cell migration, and apoptosis assays were completed. The results showed that SOD2 expression did not correlate with tumor aggressiveness nor SOD2 genotype. We demonstrated that the Ala-SOD2 allele was associated with marked induction of EMT indicated by higher Snail and vimentin, lower E-cadherin, and increased cell migration, when compared to Val-SOD2 allele or Neo control cells. Ala-SOD2 SNP cells exhibited increased levels of total ROS and superoxide and were more sensitive to co-treatment with H2O2 and MSKE, which led to reduced cell growth and increased apoptosis. Additionally, MSKE inhibited Ala-SOD2 SNP-mediated EMT. Our data indicates that treatment with a combination of H2O2-generative drugs, such as certain chemotherapeutics and antioxidants such as MSKE that targets superoxide, hold promising therapeutic potential to halt PCa progression in the future.
Seismic Response of RC Frames with a Soft First Story Retrofitted with Hysteretic Dampers under Near-Fault Earthquakes
Authors: Mota-Páez Santiago , Escolano-Margarit David , Benavent-Climent Amadeo
Published: February 2021

Reinforced concrete (RC) frame structures with open first stories and masonry infill walls at the upper stories are very common in seismic areas. Under strong earthquakes, most of the energy dissipation demand imposed by the earthquake concentrates in the first story, and this eventually leads the building to collapse. A very efficient and cost-effective solution for the seismic upgrading of this type of structure consists of installing hysteretic dampers in the first story. This paper investigates the response of RC soft-story frames retrofitted with hysteretic dampers subjected to near-fault ground motions in terms of maximum displacements and lateral seismic forces and compares them with those obtained by far-field earthquakes. It is found that for similar levels of total seismic input energy, the maximum displacements in the first story caused by near-fault earthquakes are about 1.3 times larger than those under far-field earthquakes, while the maximum inter-story drift in the upper stories and the distribution and values of the lateral forces are scarcely affected. It is concluded that the maximum displacements can be easily predicted from the energy balance of the structure by using appropriate values for the parameter that reflects the influence of the impulsivity of the ground motion: the so-called equivalent number of cycles.
Structure of Microbial Communities When Complementary Effluents Are Anaerobically Digested
Authors: Eusébio Ana , Neves André , Marques Isabel Paula
Published: February 2021

Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents—piggery effluent and olive mill wastewater—through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples.
Effects of Driving Frequency and Voltage on the Performances of Vibrating Mesh Nebulizers
Authors: Moon Sang-Hyub , Chang Kyung Hwa , Park Hyun Mok , Park Bong Joo , Yoo Sun Kook , Nam Ki Chang
Published: February 2021

The functional components of vibrating mesh nebulizers are a piezoelectric ceramic with a mesh mounted on one side, a reservoir, and a driving circuit. The piezoelectric material vibrates at a specific intrinsic frequency, and when the mechanical resonance frequency of the piezoelectric ceramic and the frequency of the applied electrical signal match, the vibration amplitude of the ceramic is greatest. In the present study, nebulizing performances were tested with respect to driving voltage amplitude after automatic resonance frequency tuning (ARFT) and/or impedance matching (IM) for salbutamol and glycerol solutions. A 1% mismatch of resonance frequency reduced the output rate by 11.0~30.1% and increased particle size by 1.6~7.7% and power consumption increased by 6.6~13.6%. Driving at 30 Vpp after ARFT and IM increased output rate by 45% and decreased power consumption by 31% compared with operation at nominal resonance frequency without IM at 50 Vpp. Nebulization of viscous solutions was also enhanced by applying ARFT with IM. The study shows the application of ARFT with IM improves vibrating mesh nebulizer performance and reduces power consumption.
HARVEST: High-Resolution Haptic Vest and Fingertip Sensing Glove That Transfers Tactile Sensation of Fingers to the Back
Authors: Moriyama Taha , Kajimoto Hiroyuki
Published: February 2021

Human fingertips are densely populated with tactile receptors and are hence incredibly sensitive. However, wearing gloves on the fingers drastically reduces the tactile information available to the fingertips, such as the texture and shape of the object, and makes it difficult to perform dexterous work. As a solution, in this study, we developed a high-resolution haptic vest that transfers the tactile sensation of the fingertips to the back. The haptic vest contains 80 voice-coil type vibrators which are located at each of the two discrimination thresholds on the back and can be driven independently. The tactile sensation of the fingertips is transferred to the back using the developed haptic vest in combination with a sensing glove that can detect the pressure distribution on the finger skin at up to 100 points. Different experiments were conducted to validate the performance of the proposed haptic vest and sensing gloves. The use of the haptic vest and the sensing glove enabled the user to perceive the shape of a planar object more accurately when compared to the case where the user wore only the glove.
Coupled D33 Mode-Based High Performing Bio-Inspired Piezoelectric MEMS Directional Microphone
Authors: Rahaman Ashiqur , Jung Haeil , Kim Byungki
Published: February 2021

Microelectromechanical system (MEMS) directional microphones have been identified as having use in multi-projected virtual reality applications such as virtual meetings for projecting cameras. In these applications, the acoustic sensitivity plays a vital role as it biases the directional sensing, signal-to-noise ratio (SNR) and self-noise. The acoustic sensitivity is the multiplied outcome of the mechanical sensitivity and the electrical sensitivity. As the dimensions are limited in MEMS technology, the improvement of the acoustic sensitivity by reflecting the mechanical as well as electrical domains is a challenge. This paper reports on a new formation of the D33 mode, the coupled D33 mode, based on piezoelectric sensing to improve the acoustic functionalities. The unique advancement of the proposed D33 mode is that it allows multiple spans of the regular D33 mode to perform together, despite this increasing the diaphragm’s dimensions. At a reduced diaphragm size, the orientation of the coupled D33 mode realizes the maximum conversion of the mechanical deflection into electrical sensitivity. The significance of the proposed D33 mode in comparison to the regular D33 mode is simulated using COMSOL Multiphysics. Then, for a proof–of–concept, the experimental validation is carried out using a piezoelectric MEMS directional microphone inspired by the ears of the fly Ormia ochracea. In both ways, the results are found to be substantially improved in comparison with the regular approach of the D33 mode, showing the novelty of this work.
Augmented Reality Visualization of Modal Analysis Using the Finite Element Method
Authors: Yavuz Erkek Merve , Erkek Selim , Jamei Elmira , Seyedmahmoudian Mehdi , Stojcevski Alex , Horan Ben
Published: February 2021

Modal analysis provides the dynamic behavior of an object or structure, and is often undertaken using the Finite Element Method (FEM) due to its ability to deal with arbitrary geometries. This article investigates the use of Augmented Reality (AR) to provide the in situ visualization of a modal analysis for an aluminum impeller. Finite Element Analysis (FEA) software packages regularly use heat maps and shape deformation to visualize the outcomes of a given simulation. AR allows the superimposition of digital information on a view of the real-world environment, and provides the opportunity to overlay such simulation results onto real-world objects and environments. The presented modal analysis undertaken herein provides natural frequencies and the corresponding deformation of an aluminum impeller. The results indicate the ability for the design part and finite element analysis results to be viewed on the physical part. A mobile AR-FEA-based system was developed for Modal Analysis result visualization. This study offers designers and engineers a new way to visualize such simulation results.
The Radioactivity of Thorium Incandescent Gas Lantern Mantles
Authors: Hassan Halmat Jalal , Hashim Suhairul , Sanusi Mohamad Syazwan Mohd , Bradley David Andrew , Alsubaie Abdullah , Tenorio Rafael Garcia , Bakri Noor Fitriah , Tahar Rozman Mohd
Published: February 2021

The use of thorium in providing the intense white luminescence emitted from gas mantles, has a history of some 130 years, the initial application pre-dating by several decades large-scale urban electric lighting. Accordingly, the thoriated gas mantle has proved itself to be of enormous utility, remaining popular in more rural areas well into the 20th century, continuing to enjoy use in campsites and street night markets lanterns until today. The discovery of thorium in 1828 preceded the discovery of radioactivity, with subsequent little appreciation initially of any potential harm from exposure to radioactivity. Study has been made herein of small quantities of five different types of the thoriated gas mantle, all purchased online devoid of any control measures. Several approaches were used concerning the 232Th activity and dose consequence. First, the activity of 232Th was estimated using an HPGe detector, with sample M5 providing the greatest activity at 1.25 × 104 Bq, exceeding the exemption limit for thorium in a mantle. Compared to sample M5, samples M1 to M4 were low in radioactivity, from 5.1 ± 1.31 to 16.33 ± 1.92 Bq. Moreover, the thorium content in M5 constituted 50% of the mantle mass, somewhat greater than previous literature values. The dose equivalent rate on the surface of a single M5 mantle was found to be 0.68 µSv/h, while at the surface of a pack of six the level was 1.9 µSv/h. Monte Carlo simulation codes have been used to obtain organ equivalent and effective dose rates, the greatest close contact (10 cm) exposure to an unlit mantle being to the thymus, at 0.68 µSv/h and 0.62 µSv/h for a male and female phantom respectively. Accordingly, with packages of thoriated gas mantles potentially giving rise to non-negligible equivalent doses, greater incorporation of controls on the sale of such items in national radiation protection legislation would seem worthy of consideration.
Towards Detection of Interest Using Physiological Sensors
Authors: Babiker Areej , Baashar Yahia , Alkahtani Ammar Ahmed , Faye Ibrahima , Alkawsi Gamal
Published: February 2021

The positive effects of interest on different aspects, e.g., learning and education, economy, psychological well-being, and social relations, have been widely addressed by many psychological and physiological studies in the last two decades. While the psychological work has investigated this impact of interest theoretically, the physiological studies have focused more on the modulatory effects. However, some studies have addressed both sides of the effects. In this work, we conduct a comprehensive review of physiological studies on interest detection, from different perspectives carried out between 2003 and 2019. A lack of connection between the psychological and physiological studies was identified. Therefore, this paper aims to integrate the unique psychological and physiological aspects and characteristics of interest to form a base for future research by considering the pros and cons of the included studies. For example, considering the two types of interest (situational and individual) the detected interest in learning, gaming, and advertisement’s physiological experiments could be referring specifically to situational interest. Hence, bridging the gap between both physiological and psychological studies is essential for improving the research on interest. Furthermore, we propose several suggestions for future work direction.
The On-Line Integrated Mesoscale Chemistry Model BOLCHEM
Authors: Cesari Rita , Landi Tony Christian , D’Isidoro Massimo , Mircea Mihaela , Russo Felicita , Malguzzi Piero , Tampieri Francesco , Maurizi Alberto
Published: February 2021

This work presents the on-line coupled meteorology–chemistry transport model BOLCHEM, based on the hydrostatic meteorological BOLAM model, the gas chemistry module SAPRC90, and the aerosol dynamic module AERO3. It includes parameterizations to describe natural source emissions, dry and wet removal processes, as well as the transport and dispersion of air pollutants. The equations for different processes are solved on the same grid during the same integration step, by means of a time-split scheme. This paper describes the model and its performance at horizontal resolution of 0.2∘× 0.2∘ over Europe and 0.1∘× 0.1∘ in a nested configuration over Italy, for one year run (December 2009–November 2010). The model has been evaluated against the AIRBASE data of the European Environmental Agency. The basic statistics for higher resolution simulations of O3, NO2 and particulate matter concentrations (PM2.5 and PM10) have been compared with those from Copernicus Atmosphere Monitoring Service (CAMS) ensemble median. In summer, for O3 we found a correlation coefficient R of 0.72 and mean bias of 2.15 over European domain and a correlation coefficient R of 0.67 and mean bias of 2.36 over Italian domain. PM10 and PM2.5 are better reproduced in the winter, the latter with a correlation coefficient R of 0.66 and the mean bias MB of 0.35 over Italian domain.
PM10-Bound Sugars: Chemical Composition, Sources and Seasonal Variations
Authors: Gonçalves Cátia , Rienda Ismael Casotti , Pina Noela , Gama Carla , Nunes Teresa , Tchepel Oxana , Alves Célia
Published: February 2021

The presence of anhydrosugars and sugar alcohols in airborne articulate matter <10 µm (PM10) samples collected between December 2018 and June 2019 was studied for two urban environments in Coimbra. Anhydrosugars were used to estimate the biomass burning contribution, and sugar alcohols were investigated regarding biological sources. Anhydrosugars contributed more than sugar alcohols to the total sugars, mainly levoglucosan. Higher levoglucosan concentrations were linked with the use of biomass-fueled heating appliances, mainly during cold periods. A significant contribution from biomass burning smoke was registered, accounting for 20% to 23% of the PM10 mass in the colder period. Xylitol presented higher concentrations in the colder period and was well correlated with levoglucosan, indicating a common origin. Mannitol and arabitol were well correlated with each other but did not present any kind of correlation with anhydrosugars or xylitol, suggesting a natural source. A quantitative estimation based on the concentration of ambient tracers (mannitol) was evaluated, and the results reveal that, for the two sites, the fungal spore relative contribution to PM10 (roadside site: 2.7% to 2.8%; urban background: 1.9% to 2.7%) and OC mass (roadside site: 6.2% to 8.1%; urban background: 3.9% to 7.5%) was significant and always higher in the warmer period.
Preoperative Planning of Spiral Intestinal Lengthening and Tailoring: A Geometrical Approach
Authors: Coletta Riccardo , Mussi Elisa , Uccheddu Francesca , Volpe Yary , Morabito Antonino
Published: February 2021

Short bowel syndrome is a pathological condition resulting from extensive resection of the intestine, generally performed due to congenital abnormalities, Crohn’s disease, mesenteric ischemia, or neoplasms. The main consequence of this syndrome is a reduction of intestinal absorption, which causes malnutrition and dehydration. In the most severe cases, specific and complex surgical procedures are requested to manage the syndrome. Such procedures consist of the intestinal lengthening, with lead to an increase of absorptive mucosal surface and intestinal transit time and an overall enhancement of intestinal absorption. One of the most promising surgical procedures is spiral intestinal lengthening and tailoring, which consists of a spiral incision of the intestinal wall and in the elongation longitudinally of the intestine by sliding one flap over the other. The final intestinal lengthening is strictly dependent on a series of parameters, some of which are defined by the surgeon. The present paper proposes a mathematical model, based on patient specific anatomical data, which aims to help the surgeon in defining the optimal parameters for the intervention and in foreseeing its outcomes from the preoperative planning phase. Such a tool can assist the physician in the surgery room by improving the procedure and reducing surgical times.
Surface Modification of Basalt Fibres with ZnO Nanorods and Its Effect on Thermal and Mechanical Properties of PLA-Based Composites
Authors: Sbardella Francesca , Martinelli Andrea , Di Lisio Valerio , Bavasso Irene , Russo Pietro , Tirillò Jacopo , Sarasini Fabrizio
Published: February 2021

The composites based on basalt fibres and poly(lactic acid) (PLA) show promising applications in biomedical and automotive fields, but their mechanical performance is still largely hindered by poor interfacial properties. Zinc oxide nanorods have been successfully used to tune the PLA/basalt fibre interface by growing them on commercially available basalt fabrics. The hierarchical fibres significantly enhanced the mechanical properties of PLA-based composites, especially their flexural strength and stiffness. These values are 26% and 22% higher than those of unmodified basalt/PLA composites, and 24% and 34% higher than those of glass/PLA composites used as a baseline. The increase in tensile and flexural properties hinges on the mechanical interlocking action promoted by ZnO nanorods and on the creation of a compact transcrystallinity structure. A degradation of PLA matrix was detected but it was positively counteracted by the better interfacial stress transfer. This study offers a novel approach for modifying the fibre–matrix interface of biocomposites intended for high-performance applications.
A Changing Paradigm in Heart Transplantation: An Integrative Approach for Invasive and Non-Invasive Allograft Rejection Monitoring
Authors: Giarraputo Alessia , Barison Ilaria , Fedrigo Marny , Burrello Jacopo , Castellani Chiara , Tona Francesco , Bottio Tomaso , Gerosa Gino , Barile Lucio , Angelini Annalisa
Published: February 2021

Cardiac allograft rejection following heart transplantation is challenging to diagnose. Tissue biopsies are the gold standard in monitoring the different types of rejection. The last decade has seen an increased emphasis on identifying non-invasive methods to improve rejection diagnosis and overcome tissue biopsy invasiveness. Liquid biopsy, as an efficient non-invasive diagnostic and prognostic oncological monitoring tool, seems to be applicable in heart transplant follow-ups. Moreover, molecular techniques applied on blood can be translated to tissue samples to provide novel perspectives on tissue and reveal new diagnostic and prognostic biomarkers. This review aims to provide a comprehensive overview of the state-of-the-art of the new methodologies in cardiac allograft rejection monitoring and investigate the future perspectives on invasive and non-invasive rejection biomarkers identification. We reviewed literature from the most used scientific databases, such as PubMed, Google Scholar, and Scopus. We extracted 192 papers and, after a selection and exclusion process, we included in the review 81 papers. The described limitations notwithstanding, this review show how molecular biology techniques and omics science could be deployed complementarily to the histopathological rejection diagnosis on tissue biopsies, thus representing an integrated approach for heart transplant patients monitoring.
Immunosuppressive Treatment in Antiphospholipid Syndrome: Is It Worth It?
Authors: Mormile Ilaria , Granata Francescopaolo , Punziano Alessandra , de Paulis Amato , Rossi Francesca Wanda
Published: February 2021

The antiphospholipid syndrome (APS) is characterized by the development of venous and/or arterial thrombosis and pregnancy morbidity in patients with persistent antiphospholipid antibodies (aPL). Catastrophic antiphospholipid syndrome (CAPS) is a life-threatening form of APS occurring in about 1% of cases. Lifelong anticoagulation with vitamin K antagonists remains the cornerstone of the therapy for thrombotic APS, but frequently the use of anticoagulation may be problematic due to the increased risk of bleeding, drug interactions, or comorbidities. Immunosuppressant drugs are widely used to treat several autoimmune conditions, in which their safety and effectiveness have been largely demonstrated. Similar evidence in the treatment of primary APS is limited to case reports or case series, and studies on a large scale lack. Immunomodulatory drugs may be an emerging tool in managing such particular situations, like refractory obstetrical complications, CAPS, or so-called APS non-criteria manifestations. In addition, immunomodulatory drugs may be useful in patients experiencing recurrent thromboembolic events despite optimized anticoagulant therapy. We did a comprehensive review of literature analyzing the possible role of immunomodulation in primary APS to provide a broad overview of potentially safe and effective target treatments for managing this devastating disease.
Pancreastatin Reduces Alternatively Activated Macrophages, Disrupts the Epithelial Homeostasis and Aggravates Colonic Inflammation. A Descriptive Analysis
Authors: Eissa Nour , Elgazzar Omar , Hussein Hayam , Hendy Geoffrey N. , Bernstein Charles N. , Ghia Jean-Eric
Published: February 2021

Ulcerative colitis (UC) is characterized by modifying alternatively activated macrophages (AAM) and epithelial homeostasis. Chromogranin-A (CHGA), released by enterochromaffin cells, is elevated in UC and is implicated in inflammation progression. CHGA can be cleaved into several derived peptides, including pancreastatin (PST), which is involved in proinflammatory mechanisms. Previously, we showed that the deletion of Chga decreased the onset and severity of colitis correlated with an increase in AAM and epithelial cells’ functions. Here, we investigated PST activity in colonic biopsies of participants with active UC and investigated PST treatment in dextran sulfate sodium (DSS)-induced colitis using Chga−/− mice, macrophages, and a human colonic epithelial cells line. We found that the colonic protein expression of PST correlated negatively with mRNA expression of AAM markers and tight junction (TJ) proteins and positively with mRNA expression of interleukin (IL)-8, IL18, and collagen in human. In a preclinical setting, intra-rectal administration of PST aggravated DSS-induced colitis by decreasing AAM’s functions, enhancing colonic collagen deposition and disrupting epithelial homeostasis in Chga+/+ and Chga−/− mice. This effect was associated with a significant reduction in AAM markers, increased colonic IL-18 release, and decreased TJ proteins’ gene expression. In vitro, PST reduced Chga+/+ and Chga−/− AAM polarization and decreased anti-inflammatory mediators’ production. Conditioned medium harvested from PST-treated Chga+/+ and Chga−/− AAM reduced Caco-2 cell migration, viability, proliferation, and mRNA levels of TJ proteins and increased oxidative stress-induced apoptosis and proinflammatory cytokines release. In conclusion, PST is a CHGA proinflammatory peptide that enhances the severity of colitis and the inflammatory process via decreasing AAM functions and disrupting epithelial homeostasis.
Regulation of Fibroblast Cell Polarity by Src Tyrosine Kinase
Authors: Katoh Kazuo
Published: February 2021

Src protein tyrosine kinases (SFKs) are a family of nonreceptor tyrosine kinases that are localized beneath the plasma membrane and are activated during cell adhesion, migration, and elongation. Due to their involvement in the activation of signal transduction cascades, SFKs have been suggested to play important roles in the determination of cell polarity during cell extension and elongation. However, the mechanism underlying Src-mediated polarity formation remains unclear. The present study was performed to investigate the mechanisms underlying Src-induced cell polarity formation and cell elongation using Src knockout fibroblasts (SYFs) together with an inhibitor of Src. Normal and Src knockout fibroblasts were also transfected with a wild-type c-Src, dominant negative c-Src, or constitutively active c-Src gene to analyze the changes in cell morphology. SYF cells cultured on a glass substrate elongated symmetrically into spindle-shaped cells, with the formation of focal adhesions at both ends of the cells. When normal fibroblasts were treated with Src Inhibitor No. 5, a selective inhibitor of Src tyrosine kinases, they elongated into symmetrical spindle-shaped cells, similar to SYF cells. These results suggest that cell polarity during extension and elongation may be regulated by SFKs and that the expression and regulation of Src are important for the formation of polarity during cell elongation.
Anti-NLRP3 Inflammasome Natural Compounds: An Update
Authors: Liu Baolong , Yu Jiujiu
Published: February 2021

The nucleotide-binding domain and leucine-rich repeat related (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome is a multimeric protein complex that recognizes various danger or stress signals from pathogens, the host, and the environment, leading to activation of caspase-1 and inducing inflammatory responses. This pro-inflammatory protein complex plays critical roles in pathogenesis of a wide range of diseases including neurodegenerative diseases, autoinflammatory diseases, and metabolic disorders. Therefore, intensive efforts have been devoted to understanding its activation mechanisms and to searching for its specific inhibitors. Approximately forty natural compounds with anti-NLRP3 inflammasome properties have been identified. Here, we provide an update about new natural compounds that have been identified within the last three years to inhibit the NLRP3 inflammasome and offer an overview of the underlying molecular mechanisms of their anti-NLRP3 inflammasome activities.
Nanomedicine-Based Strategies Assisting Photodynamic Therapy for Hypoxic Tumors: State-of-the-Art Approaches and Emerging Trends
Authors: Shih Chun-Yan , Wang Pei-Ting , Su Wu-Chou , Teng Hsisheng , Huang Wei-Lun
Published: February 2021

Since the first clinical cancer treatment in 1978, photodynamic therapy (PDT) technologies have been largely improved and approved for clinical usage in various cancers. Due to the oxygen-dependent nature, the application of PDT is still limited by hypoxia in tumor tissues. Thus, the development of effective strategies for manipulating hypoxia and improving the effectiveness of PDT is one of the most important area in PDT field. Recently, emerging nanotechnology has benefitted progress in many areas, including PDT. In this review, after briefly introducing the mechanisms of PDT and hypoxia, as well as basic knowledge about nanomedicines, we will discuss the state of the art of nanomedicine-based approaches for assisting PDT for treating hypoxic tumors, mainly based on oxygen replenishing strategies and the oxygen dependency diminishing strategies. Among these strategies, we will emphasize emerging trends about the use of nanoscale metal–organic framework (nMOF) materials and the combination of PDT with immunotherapy. We further discuss future perspectives and challenges associated with these trends in both the aspects of mechanism and clinical translation.
The Ecology of the Zebra Finch Makes It a Great Laboratory Model but an Outlier amongst Passerine Birds
Authors: Griffith Simon C. , Ton Riccardo , Hurley Laura L. , McDiarmid Callum S. , Pacheco-Fuentes Hector
Published: February 2021

Zebra Finches have become the most widely researched bird species outside of those used in agricultural production. Their adoption as the avian model of choice is largely down to a number of characteristics that make them easy to obtain and use in captivity. The main point of our paper is that the very characteristics that make the Zebra Finch a highly amenable laboratory model species mean that it is by definition different from many other passerine birds, and therefore not a good general model for many research areas. The Zebra Finch is likely to be particularly resilient to the effects of stress early in life, and is likely to show great flexibility in dealing with a wide variety of conditions later in life. Whilst it is tempting for researchers to turn to species such as the Zebra Finch, that can be the focus of manipulative work in the laboratory, we caution that the findings of such studies may confound our understanding of general avian biology. The Zebra Finch will remain an excellent species for laboratory work, and our paper should help to direct and interpret future work in the laboratory and the field.