bluuebunny/crossref_metadata_2025 huggingface dataset:
The Psychological Impact of the COVID-19 Lockdown: A Comparison between Caregivers of Autistic and Non-Autistic Individuals in Italy
Authors: Fusar-Poli Laura , Martinez Miriam , Surace Teresa , Meo Valeria , Patania Federica , Avanzato Chiara , Signorelli Maria Salvina , Aguglia Eugenio
Published: January 2022

The COVID-19 outbreak has disrupted the daily routine of the population worldwide, including autistic people and their caregivers, with severe consequences on mental health. On one hand, the reduced social contacts and the interruption of outpatient and daycare services during the lockdown have represented a real challenge for autistic people and their caregivers. On the other hand, confinement has allowed individuals to spend more time pursuing their interests and stay home with their family members without feeling the pressure of social expectations. The present study aimed to compare the levels of personal wellbeing, family distress, insomnia, and resilience between caregivers of autistic people and caregivers of people with other neurodevelopmental, psychiatric, or relational disabilities. A web survey was completed by 383 participants, of which 141 were primary caregivers of autistic people. We did not find any significant difference between caregivers of autistic and non-autistic people in any of the considered psychological variables. Lower age of the autistic family member and lower resilience levels were significantly associated with higher individual distress in the group of caregivers of autistic people. Our findings do not corroborate the hypothesis that caregivers of autistic individuals have had more severe consequences than other caregivers during the lockdown. However, they confirm the importance of promoting resilient coping strategies in autistic people and their caregivers.
Decreased Balance Function in School-Aged Children with Behavioral Problems
Authors: Matsunaga Naomichi , Ito Tadashi , Ito Yuji , Mizusawa Jun , Gu Yingzhi , Sanada Shota , Shirai Yuya , Takahashi Daiki , Ochi Nobuhiko , Noritake Koji , Sugiura Hideshi
Published: January 2022

Children with behavioral problems have a high risk of impaired motor performance. However, the characteristics of balance functions and their associations with behavioral traits are unclear in this population. This study aimed to evaluate balance functions and their relationships with the degree of behavioral problems in school-aged children. A total of 209 children, aged 6–10 years, were divided into two groups, those with and those without behavioral problems, using the Strengths and Difficulties Questionnaire (SDQ). Physical assessments included the one-leg standing test (OLST), the two-step test, and the five-times-sit-to-stand test. We compared the data between groups and assessed for correlations in terms of total difficulties and the SDQ subscale scores. Children with behavioral problems showed significantly reduced the OLST results (p < 0.001) and the two-step test results (p = 0.008). The five-times-sit-to-stand test results did not show significant differences between groups. The OLST results were significantly correlated with emotional symptoms (r = −0.22, p < 0.001), hyperactivity/inattention (r = −0.29, p < 0.001), peer relationship problems (r = −0.22, p < 0.001), and total difficulties (r = −0.32, p < 0.001). Meanwhile, the two-step test results showed no significant correlation with the SDQ scores. Children with behavioral problems have poor balance function, thereby increasing the risk for instability. This suggests that the balance function of children with behavioral problems needs to be considered.
Serum Levels of Lipids and Selected Aminothiols in Epileptic Children—A Pilot Case-Control Study
Authors: Sarecka-Hujar Beata , Szołtysek-Bołdys Izabela , Kopyta Ilona
Published: January 2022

Background: Standard treatment of epileptic seizures involves the use of antiepileptic drugs (AEDs). Both AEDs themselves and treatment duration may influence the levels of biochemical parameters, e.g., lipids or homocysteine (HCys), that may increase the risk of cardiovascular diseases. The aim of the present study was to compare the levels of lipid parameters, as well as the concentrations of selected aminothiols (i.e., HCys, cysteine, and glutathione) between epileptic children treated with multiple AEDs and children without epilepsy. Methods: In the study, 21 children with epilepsy treated with two or more AEDs for at least 6 months (8 girls and 13 boys, mean age 7.03 ± 4.51) and 23 children without epilepsy (7 girls and 16 boys, mean age 7.54 ± 3.90) were prospectively analyzed. Lipid parameters, i.e., total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL), and levels of selected aminothiols were determined in the blood serum. Results: No differences in the mean levels of lipid parameters and in the mean values of lipid ratios (TC/HDL, TG/HDL, LDL/HDL) were observed between the total groups as well as in the sex subgroups. HCys and cysteine levels did not differ between the patients and controls. We observed significantly lower levels of glutathione in children with epilepsy than in children without epilepsy (1.49 ± 0.35 µmol/L vs. 2.39 ± 1.17 µmol/L, respectively) (p < 0.001). Glutathione level was also lower in boys with epilepsy than in boys without epilepsy (p = 0.007). Similarly, epileptic girls had statistically decreased levels of glutathione when compared to girls without epilepsy (p = 0.006). Conclusions: A lower level of glutathione is observed in pediatric patients with epilepsy treated with two or more AEDs for at least 6 months. This indicates the oxidative stress of the patients treated with AEDs, which in turn may affect their well-being, and in the case of chronic occurrence resulting from long-term treatment, also on the function of the liver and the condition of the cardiovascular system.
CircFISH: A Novel Method for the Simultaneous Imaging of Linear and Circular RNAs
Authors: Koppula Aakash , Abdelgawad Ahmed , Guarnerio Jlenia , Batish Mona , Parashar Vijay
Published: January 2022

Circular RNAs (circRNAs) are regulatory RNAs which have recently been shown to have clinical significance in several diseases, including, but not limited to, various cancers, neurological diseases and cardiovascular diseases. The function of such regulatory RNAs is largely dependent on their subcellular localization. Several circRNAs have been shown to conduct antagonistic roles compared to the products of the linear isoforms, and thus need to be characterized distinctly from the linear RNAs. However, conventional fluorescent in situ hybridization (FISH) techniques cannot be employed directly to distinguish the signals from linear and circular isoforms because most circRNAs share the same sequence with the linear RNAs. In order to address this unmet need, we adapted the well-established method of single-molecule FISH by designing two sets of probes to differentiate the linear and circular RNA isoforms by virtue of signal colocalization. We call this method ‘circular fluorescent in situ hybridization’ (circFISH). Linear and circular RNAs were successfully visualized and quantified at a single-molecule resolution in fixed cells. RNase R treatment during the circFISH reduced the levels of linear RNAs while the circRNA levels remain unaltered. Furthermore, cells with shRNAs specific to circRNA showed the loss of circRNA levels, whereas the linear RNA levels were unaffected. The optimization of the in-situ RNase R treatment allowed the multiplexing of circFISH to combine it with organelle staining. CircFISH was found to be compatible with multiple sample types, including cultured cells and fresh-frozen and formalin-fixed tissue sections. Thus, we present circFISH as a versatile method for the simultaneous visualization and quantification of the distribution and localization of linear and circular RNA in fixed cells and tissue samples.
Machine Learning Using Real-World and Translational Data to Improve Treatment Selection for NSCLC Patients Treated with Immunotherapy
Authors: Prelaj Arsela , Boeri Mattia , Robuschi Alessandro , Ferrara Roberto , Proto Claudia , Lo Russo Giuseppe , Galli Giulia , De Toma Alessandro , Brambilla Marta , Occhipinti Mario , Manglaviti Sara , Beninato Teresa , Bottiglieri Achille , Massa Giacomo , Zattarin Emma , Gallucci Rosaria , Galli Edoardo Gregorio , Ganzinelli Monica , Sozzi Gabriella , de Braud Filippo G. M. , Garassino Marina Chiara , Restelli Marcello , Pedrocchi Alessandra Laura Giulia , Trovo' Francesco
Published: January 2022

(1) Background: In advanced non-small cell lung cancer (aNSCLC), programmed death ligand 1 (PD-L1) remains the only biomarker for candidate patients to immunotherapy (IO). This study aimed at using artificial intelligence (AI) and machine learning (ML) tools to improve response and efficacy predictions in aNSCLC patients treated with IO. (2) Methods: Real world data and the blood microRNA signature classifier (MSC) were used. Patients were divided into responders (R) and non-responders (NR) to determine if the overall survival of the patients was likely to be shorter or longer than 24 months from baseline IO. (3) Results: One-hundred sixty-four out of 200 patients (i.e., only those ones with PD-L1 data available) were considered in the model, 73 (44.5%) were R and 91 (55.5%) NR. Overall, the best model was the linear regression (RL) and included 5 features. The model predicting R/NR of patients achieved accuracy ACC = 0.756, F1 score F1 = 0.722, and area under the ROC curve AUC = 0.82. LR was also the best-performing model in predicting patients with long survival (24 months OS), achieving ACC = 0.839, F1 = 0.908, and AUC = 0.87. (4) Conclusions: The results suggest that the integration of multifactorial data provided by ML techniques is a useful tool to select NSCLC patients as candidates for IO.
Pathological Significance of GLUT-1 Expression in Breast Cancer Cells in Diabetic and Obese Patients: The French Guiana Study
Authors: Suteau Valentin , Bukasa-Kakamba John , Virjogh-Cenciu Beatrice , Adenis Antoine , Sabbah Nadia , Drak Alsibai Kinan
Published: January 2022

The prevalence of obesity and type 2 diabetes is higher in French Guiana compared to mainland France. These metabolic disorders are associated with an increased risk of cancer. One of the factors involved is hyperinsulinemia that promotes the action of glucose transporter 1 (GLUT-1). The objective of this study is to characterize the expression of GLUT-1 in breast cancers cells in diabetic and obese patients compared to those who are not and to describe the clinical and histological prognostic factors of breast cancer in this population. We conducted a monocentric study including patients with breast cancer diagnosed between 2014 and 2020. Patients were classified into three groups: diabetes, obesity, and control group. The GLUT-1 expression was assessed by immunohistochemistry. In total, 199 patients were included in this study. The median age was 53.5 years, and the median tumor size was 2.8 cm. Luminal A was the most frequent molecular type (58.1%), followed by the triple-negative type (19.9%). The breast cancer in our population was characterized by a younger age at diagnosis, more aggressive molecular types, and larger tumor size. Thus, we suggest the advancement of the age of breast cancer screening in this territory. A total of 144 patients (31 diabetes, 22 obese, and 91 control group) were included for the study of GLUT-1 expression. Overexpression of GLUT-1 was observed in 60.4% of cases and in all carcinoma in situ lesions. GLUT-1 overexpression was associated with more aggressive cancers. This overexpression is correlated with high histological grade, high proliferation index, and aggressive molecular types. Our study found no difference in GLUT-1 expression between the diabetic or obese patients and the control group. These results highlight the potential role of GLUT-1 as a tumor metabolic prognostic marker and also as an interesting target therapy, independently of patient metabolic disorder.
Keep Calm and Carry on with Extra Centrosomes
Authors: Kalkan Batuhan Mert , Ozcan Selahattin Can , Quintyne Nicholas J. , Reed Samantha L. , Acilan Ceyda
Published: January 2022

Aberrations in the centrosome number and structure can readily be detected at all stages of tumor progression and are considered hallmarks of cancer. Centrosome anomalies are closely linked to chromosome instability and, therefore, are proposed to be one of the driving events of tumor formation and progression. This concept, first posited by Boveri over 100 years ago, has been an area of interest to cancer researchers. We have now begun to understand the processes by which these numerical and structural anomalies may lead to cancer, and vice-versa: how key events that occur during carcinogenesis could lead to amplification of centrosomes. Despite the proliferative advantages that having extra centrosomes may confer, their presence can also lead to loss of essential genetic material as a result of segregational errors and cancer cells must deal with these deadly consequences. Here, we review recent advances in the current literature describing the mechanisms by which cancer cells amplify their centrosomes and the methods they employ to tolerate the presence of these anomalies, focusing particularly on centrosomal clustering.
A Few-Shot Learning Approach Assists in the Prognosis Prediction of Magnetic Resonance-Guided Focused Ultrasound for the Local Control of Bone Metastatic Lesions
Authors: Hsu Fang-Chi , Lee Hsin-Lun , Chen Yin-Ju , Shen Yao-An , Tsai Yi-Chieh , Wu Meng-Huang , Kuo Chia-Chun , Lu Long-Sheng , Yeh Shauh-Der , Huang Wen-Sheng , Shen Chia-Ning , Chiou Jeng-Fong
Published: January 2022

Magnetic resonance-guided focused ultrasound surgery (MRgFUS) constitutes a noninvasive treatment strategy to ablate deep-seated bone metastases. However, limited evidence suggests that, although cytokines are influenced by thermal necrosis, there is still no cytokine threshold for clinical responses. A prediction model to approximate the postablation immune status on the basis of circulating cytokine activation is thus needed. IL-6 and IP-10, which are proinflammatory cytokines, decreased significantly during the acute phase. Wound-healing cytokines such as VEGF and PDGF increased after ablation, but the increase was not statistically significant. In this phase, IL-6, IL-13, IP-10, and eotaxin expression levels diminished the ongoing inflammatory progression in the treated sites. These cytokine changes also correlated with the response rate of primary tumor control after acute periods. The few-shot learning algorithm was applied to test the correlation between cytokine levels and local control (p = 0.036). The best-fitted model included IL-6, IL-13, IP-10, and eotaxin as cytokine parameters from the few-shot selection, and had an accuracy of 85.2%, sensitivity of 88.6%, and AUC of 0.95. The acceptable usage of this model may help predict the acute-phase prognosis of a patient with painful bone metastasis who underwent local MRgFUS. The application of machine learning in bone metastasis is equivalent or better than the current logistic regression.
Who’s Who? Discrimination of Human Breast Cancer Cell Lines by Raman and FTIR Microspectroscopy
Authors: Santos Inês P. , Martins Clara B. , Batista de Carvalho Luís A. E. , Marques Maria P. M. , Batista de Carvalho Ana L. M.
Published: January 2022

(1) Breast cancer is presently the leading cause of death in women worldwide. This study aims at identifying molecular biomarkers of cancer in human breast cancer cells, in order to differentiate highly aggressive triple-negative from non-triple-negative cancers, as well as distinct triple-negative subtypes, which is currently an unmet clinical need paramount for an improved patient care. (2) Raman and FTIR (Fourier transform infrared) microspectroscopy state-of-the-art techniques were applied, as highly sensitive, specific and non-invasive methods for probing heterogeneous biological samples such as human cells. (3) Particular biochemical features of malignancy were unveiled based on the cells’ vibrational signature, upon principal component analysis of the data. This enabled discrimination between TNBC (triple-negative breast cancer) and non-TNBC, TNBC MSL (mesenchymal stem cell-like) and TNBC BL1 (basal-like 1) and TNBC BL1 highly metastatic and low-metastatic cell lines. This specific differentiation between distinct TNBC subtypes—mesenchymal from basal-like, and basal-like 1 with high-metastatic potential from basal-like 1 with low-metastatic potential—is a pioneer result, of potential high impact in cancer diagnosis and treatment.
Oncocytoma-Related Gene Signature to Differentiate Chromophobe Renal Cancer and Oncocytoma Using Machine Learning
Authors: Satter Khaled Bin , Tran Paul Minh Huy , Tran Lynn Kim Hoang , Ramsey Zach , Pinkerton Katheine , Bai Shan , Savage Natasha M. , Kavuri Sravan , Terris Martha K. , She Jin-Xiong , Purohit Sharad
Published: January 2022

Publicly available gene expression datasets were analyzed to develop a chromophobe and oncocytoma related gene signature (COGS) to distinguish chRCC from RO. The datasets GSE11151, GSE19982, GSE2109, GSE8271 and GSE11024 were combined into a discovery dataset. The transcriptomic differences were identified with unsupervised learning in the discovery dataset (97.8% accuracy) with density based UMAP (DBU). The top 30 genes were identified by univariate gene expression analysis and ROC analysis, to create a gene signature called COGS. COGS, combined with DBU, was able to differentiate chRCC from RO in the discovery dataset with an accuracy of 97.8%. The classification accuracy of COGS was validated in an independent meta-dataset consisting of TCGA-KICH and GSE12090, where COGS could differentiate chRCC from RO with 100% accuracy. The differentially expressed genes were involved in carbohydrate metabolism, transcriptomic regulation by TP53, beta-catenin-dependent Wnt signaling, and cytokine (IL-4 and IL-13) signaling highly active in cancer cells. Using multiple datasets and machine learning, we constructed and validated COGS as a tool that can differentiate chRCC from RO and complement histology in routine clinical practice to distinguish these two tumors.
Golgi Metal Ion Homeostasis in Human Health and Diseases
Authors: Li Jie , Wang Yanzhuang
Published: January 2022

The Golgi apparatus is a membrane organelle located in the center of the protein processing and trafficking pathway. It consists of sub-compartments with distinct biochemical compositions and functions. Main functions of the Golgi, including membrane trafficking, protein glycosylation, and sorting, require a well-maintained stable microenvironment in the sub-compartments of the Golgi, along with metal ion homeostasis. Metal ions, such as Ca2+, Mn2+, Zn2+, and Cu2+, are important cofactors of many Golgi resident glycosylation enzymes. The homeostasis of metal ions in the secretory pathway, which is required for proper function and stress response of the Golgi, is tightly regulated and maintained by transporters. Mutations in the transporters cause human diseases. Here we provide a review specifically focusing on the transporters that maintain Golgi metal ion homeostasis under physiological conditions and their alterations in diseases.
Endogenous pH 6.0 β-Galactosidase Activity Is Linked to Neuronal Differentiation in the Olfactory Epithelium
Authors: de Mera-Rodríguez José Antonio , Álvarez-Hernán Guadalupe , Gañán Yolanda , Santos-Almeida Ana , Martín-Partido Gervasio , Rodríguez-León Joaquín , Francisco-Morcillo Javier
Published: January 2022

The histochemical detection of β-galactosidase enzymatic activity at pH 6.0 (β-gal-pH6) is a widely used biomarker of cellular senescence in aging tissues. This histochemical assay also detects the presence of programmed cell senescence during specific time windows in degenerating structures of vertebrate embryos. However, it has recently been shown that this enzymatic activity is also enhanced in subpopulations of differentiating neurons in the developing central nervous system in vertebrates. The present study addressed the histochemical detection of β-gal-pH6 enzymatic activity in the developing postnatal olfactory epithelium in the mouse. This activity was detected in the intermediate layer of the olfactory epithelium. As development progressed, the band of β-gal-pH6 labeling in this layer increased in width. Immunohistochemistry and lectin histochemistry showed the β-gal-pH6 staining to be strongly correlated with the immunolabeling of the olfactory marker protein (OMP) that identifies mature olfactory sensory neurons. The cell somata of a subpopulation of differentiated olfactory neurons that were recognized with the Dolichos biflorus agglutinin (DBA) were always located inside this band of β-gal-pH6 staining. However, the β-gal-pH6 histochemical signal was always absent from the apical region where the cytokeratin-8 positive supporting cells were located. Furthermore, no β-gal-pH6 staining was found in the basal region of the olfactory epithelium where PCNA/pHisH3 immunoreactive proliferating progenitor cells, GAP43 positive immature neurons, and cytokeratin-5 positive horizontal basal cells were located. Therefore, β-gal-pH6 seems to be linked to neuronal differentiation and cannot be regarded as a biomarker of cellular senescence during olfactory epithelium development in mice.
[(WR)8WKβA]-Doxorubicin Conjugate: A Delivery System to Overcome Multi-Drug Resistance against Doxorubicin
Authors: Zoghebi Khalid , Aliabadi Hamidreza Montazeri , Tiwari Rakesh Kumar , Parang Keykavous
Published: January 2022

Doxorubicin (Dox) is an anthracycline chemotherapeutic agent used to treat breast, leukemia, and lymphoma malignancies. However, cardiotoxicity and inherent acquired resistance are major drawbacks, limiting its clinical application. We have previously shown that cyclic peptide [WR]9 containing alternate tryptophan (W) and arginine (R) residues acts as an efficient molecular transporter. An amphiphilic cyclic peptide containing a lysine (K) residue and alternative W and R was conjugated through a free side chain amino group with Dox via a glutarate linker to afford [(WR)8WKβA]-Dox conjugate. Antiproliferative assays were performed in different cancer cell lines using the conjugate and the corresponding physical mixture of the peptide and Dox to evaluate the effectiveness of synthesized conjugate compared to the parent drug alone. [(WR)8WKβA]-Dox conjugate showed higher antiproliferative activity at 10 µM and 5 µM than Dox alone at 5 μM. The conjugate inhibited the cell viability of ovarian adenocarcinoma (SK-OV-3) by 59% and the triple-negative breast cancer cells MDA-MB-231 and MCF-7 by 71% and 77%, respectively, at a concentration of 5 μM after 72 h of incubation. In contrast, Dox inhibited the proliferation of SK-OV-3, MDA-MB-231, and MCF-7 by 35%, 63%, and 57%, respectively. Furthermore, [(WR)8WKβA]-Dox conjugate (5 µM) inhibited the cell viability of Dox-resistant cells (MES-SA/MX2) by 92%, while the viability of cells incubated with free Dox was only 15% at 5 μM. Confocal microscopy images confirmed the ability of both Dox conjugate and the physical mixture of the peptide with the drug to deliver Dox through an endocytosis-independent pathway, as the uptake was not inhibited in the presence of endocytosis inhibitors. The stability of Dox conjugate was observed at different time intervals using analytical HPLC when the conjugate was incubated with 25% human serum. Half-life (t1/2) for [(WR)8WKβA]-Dox conjugate was (∼6 h), and more than 80% of the conjugate was degraded at 12 h. The release of free Dox was assessed intracellularly using the CCRF-CEM cell line. The experiment demonstrated that approximately 100% of free Dox was released from the conjugate intracellularly within 72 h. These data confirm the ability of the cyclic cell-penetrating peptide containing tryptophan and arginine residues as an efficient tool for delivery of Dox and for overcoming resistance to it.
Janus Kinase Signaling Pathway and Its Role in COVID-19 Inflammatory, Vascular, and Thrombotic Manifestations
Authors: Ravid Jonathan D. , Leiva Orly , Chitalia Vipul C.
Published: January 2022

Acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection continues to be a worldwide public health crisis. Among the several severe manifestations of this disease, thrombotic processes drive the catastrophic organ failure and mortality in these patients. In addition to a well-established cytokine storm associated with the disease, perturbations in platelets, endothelial cells, and the coagulation system are key in triggering systemic coagulopathy, involving both the macro- and microvasculatures of different organs. Of the several mechanisms that might contribute to dysregulation of these cells following SARS-CoV-2 infection, the current review focuses on the role of activated Janus kinase (JAK) signaling in augmenting thrombotic processes and organ dysfunction. The review concludes with presenting the current understanding and emerging controversies concerning the potential therapeutic applications of JAK inhibitors for ameliorating the inflammation-thrombosis phenotype in COVID-19 patients.
Studying the Influence of Mica Particle Size on the Properties of Epoxy Acrylate/Mica Composite Coatings through Reducing Mica Particle Size by the Ball-Milled Method
Authors: Da Yaling , Liu Jianxing , Gao Zixian , Xue Xiangxin
Published: January 2022

In this work, a series of epoxy acrylate (EA)/mica composite coatings were synthesized through introducing mica powders of different particle size into epoxy acrylate coatings and using an ultraviolet (UV) curing technique to investigate the influence of mica particle size on the coatings. Mica powders of different particle sizes were obtained by ball-milling for 4, 8, 12, 16, and 20 h with a planetary high-energy ball mill. The particle size and morphologies of ball-milled mica powders were characterized by laser particle size analyzer and scanning electron microscopy (SEM). The results indicated that planetary ball-milling reduced the particle size of mica powders effectively. Mica powders that were un-ball-milled and ball-milled were added into the epoxy acrylate matrix by a blending method to synthesize the organic-inorganic UV curable coatings. The optical photographs of the coatings showed greater stability of liquid mixtures with smaller particle size fillers. The chemical structures of EA/mica composite coatings were investigated by Fourier transform infrared spectroscopy (FTIR), and the conversion rate of C=C bonds was calculated. The results indicated that the C=C conversion of coatings with mica powders of smaller particle sizes was higher. Tests of mechanical properties and tests using electrochemical impedance spectroscopy (EIS) showed that pencil hardness, impact resistance, and coating resistance were improved due to the reduction of mica powders particle size.
Survival in Women with De Novo Metastatic Breast Cancer: A Comparison of Real-World Evidence from a Publicly-Funded Canadian Province and the United States by Insurance Status
Authors: Savard Marie-France , Kornaga Elizabeth N. , Kahn Adriana Matutino , Lupichuk Sasha
Published: January 2022

Metastatic breast cancer (MBC) patient outcomes may vary according to distinct health care payers and different countries. We compared 291 Alberta (AB), Canada and 9429 US patients < 65 with de novo MBC diagnosed from 2010 through 2014. Data were extracted from the provincial Breast Data Mart and from the National Cancer Institute’s SEER program. US patients were divided by insurance status (US privately insured, US Medicaid or US uninsured). Kaplan-Meier and log-rank analyses were used to assess differences in OS and hazard ratios (HR) were estimated using Cox models. Multivariate models were adjusted for age, surgical status, and biomarker profile. No difference in OS was noted between AB and US patients (HR = 0.92 (0.77–1.10), p = 0.365). Median OS was not reached for the US privately insured and AB groups, and was 11 months and 8 months for the US Medicaid and US uninsured groups, respectively. The 3-year OS rates were comparable between US privately insured and AB groups (53.28% (51.95–54.59) and 55.54% (49.49–61.16), respectively). Both groups had improved survival (p < 0.001) relative to the US Medicaid and US uninsured groups [39.32% (37.25–41.37) and 40.53% (36.20–44.81)]. Our study suggests that a universal health care system is not inferior to a private insurance-based model for de novo MBC.
Preliminary Clinical Experience with a Novel Optical–Ultrasound Imaging Device on Various Skin Lesions
Authors: Csány Gergely , Gergely László Hunor , Kiss Norbert , Szalai Klára , Lőrincz Kende , Strobel Lilla , Csabai Domonkos , Hegedüs István , Marosán-Vilimszky Péter , Füzesi Krisztián , Sárdy Miklós , Gyöngy Miklós
Published: January 2022

A compact handheld skin ultrasound imaging device has been developed that uses co-registered optical and ultrasound imaging to provide diagnostic information about the full skin depth. The aim of the current work is to present the preliminary clinical results of this device. Using additional photographic, dermoscopic and ultrasonic images as reference, the images from the device were assessed in terms of the detectability of the main skin layer boundaries and characteristic image features. Combined optical-ultrasonic recordings of various types of skin lesions (melanoma, basal cell carcinoma, seborrheic keratosis, dermatofibroma, naevus, dermatitis and psoriasis) were taken with the device (N = 53) and compared with images captured with a reference portable skin ultrasound imager. The investigator and two additional independent experts performed the evaluation. The detectability of skin structures was over 90% for the epidermis, the dermis and the lesions. The morphological and echogenicity information observed for the different skin lesions were found consistent with those of the reference ultrasound device and relevant ultrasound images in the literature. The presented device was able to obtain simultaneous in-vivo optical and ultrasound images of various skin lesions. This has the potential for further investigations, including the preoperative planning of skin cancer treatment.
Impact of Antibiotic Prophylaxis on Infection Rate after Endoscopic Ultrasound Through-the-Needle Biopsy of Pancreatic Cysts: A Propensity Score-Matched Study
Authors: Facciorusso Antonio , Arevalo-Mora Martha , Conti Bellocchi Maria Cristina , Bernardoni Laura , Ramai Daryl , Gkolfakis Paraskevas , Loizzi Domenico , Muscatiello Nicola , Ambrosi Antonio , Tartaglia Nicola , Robles-Medranda Carlos , Stasi Elisa , Ofosu Andrew , Crinò Stefano Francesco
Published: January 2022

Background: Despite weak evidence, antibiotic prophylaxis prior to endoscopic ultrasound-guided through-the-needle biopsy (EUS-TTNB) of pancreatic cystic lesions (PCLs) is routinely used in clinical practice. We aim to compare a group of patients treated with antibiotics before EUS-TTNB of PCLs and a group who did not undergo antimicrobial prophylaxis. Methods: Out of 236 patients with pancreatic cystic lesions referred to two high-volume centers between 2016 and 2021, after propensity score matching, two groups were compared: 98 subjects who underwent EUS-TTNB under antibiotic prophylaxis and 49 subjects without prophylaxis. Results: There was no difference in terms of baseline parameters between groups. Final diagnosis was serous cystadenoma in 36.7% of patients in the group not treated with prophylaxis and in 37.7% of patients in the control group, whereas IPMN and mucinous cystadenoma were diagnosed in 3 (6.1%) and 16 (32.6%) versus 6 (6.1%) and 32 (32.6%) patients in the two groups, respectively (p = 0.23). Overall, the adverse event rate was 6.1% in the group not treated with antibiotic prophylaxis and 5.1% in the control group (p = 0.49). Only a single infectious adverse event occurred in each group (p = 0.48). The diagnostic yields were 89.7% and 90.8% in the two groups (p = 0.7), and the diagnostic accuracy rate was 81.6% in both groups (p = 1.0). Conclusions: Prophylactic antibiotics do not seem to influence the risk of infection, and their routine use should be discouraged.
Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
Authors: Cherubini Sabrina , Perilli Mariagrazia , Azzini Anna Maria , Tacconelli Evelina , Maccacaro Laura , Bazaj Alda , Naso Laura , Amicosante Gianfranco ,   LTCF-Veneto Working Group, Lo Cascio Giuliana , Piccirilli Alessandra
Published: January 2022

Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of view, 43 E. coli strains collected from residents of LTCFs in Northern Italy. The most common lineage found was ST131, followed by sporadic presence of ST12, ST69, ST48, ST95, ST410 and ST1193. All strains were incubators of several virulence factors, with iss, sat, iha and senB being found in 84%, 72%, 63% and 51% of E. coli, respectively. Thirty of the ST131 analyzed were of the O25b:H4 serotype and H30 subclone. The ST131 isolates were found to be mainly associated with IncF plasmids, CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-27 and gyrA/parC/parE mutations. Metallo-β-lactamases were not found in ST131, whereas KPC-3 carbapenemase was found only in two ST131 and one ST1193. In conclusion, we confirmed the spread of extended-spectrum β-lactamase genes in E. coli ST131 isolated from colonized residents living inside LTCFs. The ST131 represents an incubator of fluoroquinolones, aminoglycosides and other antibiotic resistance genes in addition to different virulence factors.
Vertical Artifacts in Lung Ultrasonography: Some Common Clinician Questions and the Related Engineer Answers
Authors: Demi Marcello , Buda Natalia , Soldati Gino
Published: January 2022

Introduction: Vertical artifacts, including B lines, are frequently seen in a variety of lung diseases. Their sonomorphology varies in length, width, shape, and internal reverberations. The reason for this diversity is still unknown and is the cause of discussion between clinicians and ultrasound physics engineers. Aim: The aim of this work is to sum up the most common clinician observations and provide an explanation to each of them derived from ultrasound physics. Materials and Methods: Based on clinical and engineering experiences as well as data collected from relevant literature, the sonomorphology of vertical artifacts was analyzed. Thirteen questions and answers were prepared on the common sonomorphology of vertical artifacts, current nomenclature, and clinical observations. Conclusions: From a clinical standpoint, the analysis of vertical artifacts is very important and requires that further clinical studies be conducted in cooperation with engineers who specialize in physics.
Cooperative Spectrum Sensing Based on Multi-Features Combination Network in Cognitive Radio Network
Authors: Xu Mingdong , Yin Zhendong , Zhao Yanlong , Wu Zhilu
Published: January 2022

Cognitive radio, as a key technology to improve the utilization of radio spectrum, acquired much attention. Moreover, spectrum sensing has an irreplaceable position in the field of cognitive radio and was widely studied. The convolutional neural networks (CNNs) and the gate recurrent unit (GRU) are complementary in their modelling capabilities. In this paper, we introduce a CNN-GRU network to obtain the local information for single-node spectrum sensing, in which CNN is used to extract spatial feature and GRU is used to extract the temporal feature. Then, the combination network receives the features extracted by the CNN-GRU network to achieve multifeatures combination and obtains the final cooperation result. The cooperative spectrum sensing scheme based on Multifeatures Combination Network enhances the sensing reliability by fusing the local information from different sensing nodes. To accommodate the detection of multiple types of signals, we generated 8 kinds of modulation types to train the model. Theoretical analysis and simulation results show that the cooperative spectrum sensing algorithm proposed in this paper improved detection performance with no prior knowledge about the information of primary user or channel state. Our proposed method achieved competitive performance under the condition of large dynamic signal-to-noise ratio.
Optimal Control of Uniformly Heated Granular Fluids in Linear Response
Authors: Ruiz-Pino Natalia , Prados Antonio
Published: January 2022

We present a detailed analytical investigation of the optimal control of uniformly heated granular gases in the linear regime. The intensity of the stochastic driving is therefore assumed to be bounded between two values that are close, which limits the possible values of the granular temperature to a correspondingly small interval. Specifically, we are interested in minimising the connection time between the non-equilibrium steady states (NESSs) for two different values of the granular temperature by controlling the time dependence of the driving intensity. The closeness of the initial and target NESSs make it possible to linearise the evolution equations and rigorously—from a mathematical point of view—prove that the optimal controls are of bang-bang type, with only one switching in the first Sonine approximation. We also look into the dependence of the optimal connection time on the bounds of the driving intensity. Moreover, the limits of validity of the linear regime are investigated.
Evaluation of the Cost-Optimal Method Applied to Existing Schools Considering PV System Optimization
Authors: Ciacci Cecilia , Banti Neri , Di Naso Vincenzo , Bazzocchi Frida
Published: January 2022

In Italy in 2020, only 15.5% of school building heritage was retrofitted from an energy and environmental point of view. In this paper, the cost-optimal method was applied to two different school buildings belonging to the same Italian cold climate zone but characterized by different structural and technological solutions. The research aims at defining the cost-effective redevelopment solution among several ones proposed to apply to this building type. At the same time, this paper provides a critical analysis of the methodology applied, highlighting deficiencies related to a not proper evaluation of environmentally friendly retrofitting measures. In a cost-effective context, the main results show that the intervention on the heating system is more convenient than the retrofitting of the envelope. The energy saving is equal to about 35% for both considered schools. Among the different proposed requalification configurations, the adoption of PV (photovoltaic) electric generation is included. In this regard, an optimization procedure was implemented in a generative design environment to maximize energy production with reference to different design parameters. As a result, a solution with south oriented PV modules with a tilt angle of 42° and arranged in 0.7 m spaced rows proved to be the most effective.
Performance Evaluation of Multistage Fractured Horizontal Wells in Tight Gas Reservoirs at Block M, Ordos Basin
Authors: Wu Li , Zhang Jiqun , Jia Deli , Wang Shuoliang , Yan Yiqun
Published: January 2022

Block M of the Ordos Basin is a typical low-permeability tight sandstone gas accumulation. To develop these reservoirs, various horizontal well fracturing technologies, such as hydra-jet fracturing, open-hole packer multistage fracturing, and perf-and-plug multistage fracturing, have been implemented in practice, showing greatly varying performance. In this paper, six fracturing technologies adopted in Block M are reviewed in terms of principle, applicability, advantages, and disadvantages, and their field application effects are compared from the technical and economic perspectives. Furthermore, the main factors affecting the productivity of fractured horizontal wells are determined using the entropy method, the causes for the difference in application effects of the fracturing technologies are analyzed, and a comprehensive productivity impact index (CPII) in good correlation with the single-well production of fractured horizontal wells is constructed. This article provides a simple and applicable method for predicting the performance of multi-frac horizontal wells that takes multiple factors into account. The results can be used to select completion methods and optimize fracturing parameters in similar reservoirs.
Optimum Solar Panel Orientation and Performance: A Climatic Data-Driven Metaheuristic Approach
Authors: Naraghi Mohammad H. , Atefi Ehsan
Published: January 2022

This study presents an optimization platform based on the climatic data provided by the National Renewable Energy Laboratory (NREL) to determine the optimum solar panel orientation. Our optimization model is simpler to use than the clearness index model since there is no need to calculate the extraterrestrial insolation on a horizontal flat plate and the shape factor. This optimization approach is based on the hourly climatic data. It determines the optimum tilt angle and azimuth angle of a solar panel for the maximum power generation, considering the diurnal variation of climatic conditions. The hourly evaluation of insolation allows setting up a solar panel azimuth angle that responds to the peak power demand. The main data that impacts the solar panel performance consists of the solar direct normal incident (DNI), direct horizontal incident (DHI), global horizontal incident (GHI), ambient temperature, wind speed, and ground albedo, all of which were obtained from the NREL database for over twenty years. The accuracy of the optimization platform introduced in this study is scrutinized by investigating the three locations in the United States with different climatic conditions. The results based on the present optimization model show higher PV power than the general rule of thumb for south-facing panels with title angles the same as the latitude of the location. Moreover, the effect of deviations from optimum panel orientation is discussed to show the versatility of our technique. Our optimization model is easy-to-use, computationally efficient, and capable of being applied to other locations worldwide.
Preparation and Electrical Properties of Sr-Doped LaFeO3 Thin-Film Conversion Coatings for Solid Oxide Cell Steel Interconnect Applications
Authors: Frangini Stefano , Della Seta Livia , Paoletti Claudia
Published: January 2022

A study was conducted to explore the effects of Sr doping on the electrical properties of perovskite LaFeO3 thin-film protective conversion coatings grown onto a K41 ferritic stainless steel, a typical interconnect material for intermediate temperature solid oxide cell (SOC) applications. The Sr-doped coatings were prepared in La2O3- and SrO-containing molten carbonate baths with minor added amounts of nitrate salt for accelerated coating formation. For comparison purposes, undoped coatings were obtained using the same carbonate bath, with the only difference being that SrO was replaced by inert MgO. SEM/EDX and XRD analyses were used for coating characterization and confirmed the effective incorporation of Sr but not of Mg into the LaFeO3 layer. Although both the Sr-doped and undoped coatings consisted of a LaFeO3 layer grown above an inner Fe-Cr spinel, the coating thickness of the Sr-doped coating was distinctly higher, approximately 2 µm, which is twice that of the undoped coating. Electrical measurements in terms of Area-Specific Resistance (ASR) were conducted at 700 °C in air and showed that Sr-doping significantly improved the electrical conductivity of the coated K41 steel. Due to the Sr-doping, the ASR values of the coated steel dropped from 60 to 37 mΩ cm2 after 300 h of exposure, in spite of the higher Sr-doped coating thickness. The study concludes that Sr-doped thin-film perovskite coatings appear to be a promising solution for improved SOCs steel interconnect stability at intermediate temperatures.
Comparative Design and Performance Analysis of 10 kW Rare-Earth and Non-Rare Earth Flux Reversal Wind Generators
Authors: Bharathi Manne , Akuru Udochukwu Bola , Kumar Malligunta Kiran
Published: January 2022

Generators are a key technological element of the wind energy generation system. Currently, there is an increasing interest in adopting non-conventional stator-mounted permanent magnet generators, e.g., flux reversal generators (FRGs), which is a good alternative to conventional synchronous generators for medium-speed wind turbine generator applications. The usage of FRG with rare-earth (RE) permanent magnets (PMs) is increasing due to their high efficiency and high power density factors. However, RE PMs are unattractive to wind generators in terms of their cost and unpredictable market supply. In this paper, an attempt is made to study the potential of FRG with non-rare earths (ferrite PMs) for wind generators. The three-phase, 6/8 pole FRG is designed and compared with RE and NRE PMs for wind generator application at 375 r/min, 10 kW. Using 2D FEA, both the generators are compared in terms of their power generating performance with excellent overload capability. It shows that the average efficiency of the generators is approximately similar, but the torque density of NRE-FRG is only 51% that of RE-FRG. The NRE-FRG design is heavier, with the total active mass being 2.6 times higher than the RE-FRG, but with the estimated total active material cost of both generators almost the same. Moreover, the toque ripple for RE-FRG is 64% higher than for NRE-FRG. The demagnetization risk analysis was performed, and it is found that at higher temperatures, RE-FRG structures are prone to higher demagnetization risks, while it is much lower in NRE-FRGs. In summary, it is found that NRE-FRG is a suitable alternative to RE-FRG for medium-speed wind turbine generator applications in the 10-kW power range.
A Methodology for the Comparative Analysis of Hybrid Electric and All-Electric Power Systems for Urban Air Mobility
Authors: Donateo Teresa , Ficarella Antonio
Published: January 2022

The present investigation addresses the topic of Urban Air Mobility with particular reference to the air-taxi service with electrified power systems. A new and detailed methodology is proposed for the simplified design and energy analysis of conventional, hybrid-electric, and full-electric power systems for this application. The original contributions to the scientific literature on UAM are the detailed modeling approach, the evaluation of CO2 emissions with a Well-to-Wing approach as a function of the electricity Emission Intensity factor, and the comparison with road vehicles performing the same route in different driving conditions. The comparison demonstrates the advantages of a full electric air-taxi with today’s technology versus a hybrid-electric road taxi, especially in cases involving low emission intensity and unfavorable driving conditions (congested traffic, aggressive driving style, and high circuity factor values). In the case of 2035 technology, the comparison with a referenced fully electric road vehicle is detrimental to the air taxi but the values of Well-to-Wheel/Wing CO2 with the expected Emission Intensity of 90 g/kWe for the European Union are still quite low (67 g/km). The investigation also quantifies the negative effect of battery aging on the consumption of the air taxi and on the number of consecutive flights that can be performed without fully charging the battery.