bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential of Using Infrapatellar–Fat–Pad–Derived Mesenchymal Stem Cells for Therapy in Degenerative Arthritis: Chondrogenesis, Exosomes, and Transcription Regulation
Authors: Liao Hsiu-Jung , Chang Chih-Hung , Huang Chi-Ying F. , Chen Hui-Ting
Published: March 2022

Infrapatellar fat pad–derived mesenchymal stem cells (IPFP-MSCs) are a type of adipose-derived stem cell (ADSC). They potentially contribute to cartilage regeneration and modulation of the immune microenvironment in patients with osteoarthritis (OA). The ability of IPFP-MSCs to increase chondrogenic capacity has been reported to be greater, less age dependent, and less affected by inflammatory changes than that of other MSCs. Transcription-regulatory factors strictly regulate the cartilage differentiation of MSCs. However, few studies have explored the effect of transcriptional factors on IPFP-MSC-based neocartilage formation, cartilage engineering, and tissue functionality during and after chondrogenesis. Instead of intact MSCs, MSC-derived extracellular vesicles could be used for the treatment of OA. Furthermore, exosomes are increasingly being considered the principal therapeutic agent in MSC secretions that is responsible for the regenerative and immunomodulatory functions of MSCs in cartilage repair. The present study provides an overview of advancements in enhancement strategies for IPFP-MSC chondrogenic differentiation, including the effects of transcriptional factors, the modulation of released exosomes, delivery mechanisms for MSCs, and ethical and regulatory points concerning the development of MSC products. This review will contribute to the understanding of the IPFP-MSC chondrogenic differentiation process and enable the improvement of IPFP-MSC-based cartilage tissue engineering.
Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19
Authors: Li Tianyi , Soelberg Scott D. , Taylor Zachary , Sakthivelpathi Vigneshwar , Furlong Clement E. , Kim Jong-Hoon , Ahn Sang-gyeun , Han Peter D. , Starita Lea M. , Zhu Jia , Chung Jae-Hyun
Published: March 2022

Current point-of-care (POC) screening of Coronavirus disease 2019 (COVID-19) requires further improvements to achieve highly sensitive, rapid, and inexpensive detection. Here we describe an immunoresistive sensor on a polyethylene terephthalate (PET) film for simple, inexpensive, and highly sensitive COVID-19 screening. The sensor is composed of single-walled carbon nanotubes (SWCNTs) functionalized with monoclonal antibodies that bind to the spike protein of SARS-CoV-2. Silver electrodes are silkscreen-printed on SWCNTs to reduce contact resistance. We determine the SARS-CoV-2 status via the resistance ratio of control- and SARS-CoV-2 sensor electrodes. A combined measurement of two adjacent sensors enhances the sensitivity and specificity of the detection protocol. The lower limit of detection (LLD) of the SWCNT assay is 350 genome equivalents/mL. The developed SWCNT sensor shows 100% sensitivity and 90% specificity in clinical sample testing. Further, our device adds benefits of a small form factor, simple operation, low power requirement, and low assay cost. This highly sensitive film sensor will facilitate rapid COVID-19 screening and expedite the development of POC screening platforms.
Hippocampal Subfields and White Matter Connectivity in Patients with Subclinical Geriatric Depression
Authors: Lee Jeonghwan , Ju Gawon , Park Hyemi , Chung Seungwon , Son Jung-Woo , Shin Chul-Jin , Lee Sang Ick , Kim Siekyeong
Published: March 2022

Despite an abundance of research related to the functional and structural changes of the brain in patients with geriatric depression, knowledge related to early alterations such as decreased white matter connectivity and their association with cognitive decline remains lacking. We aimed to investigate early alterations in hippocampal microstructure and identify their associations with memory function in geriatric patients with subclinical depression. Nineteen participants with subclinical geriatric depression and 19 healthy controls aged ≥65 years exhibiting general cognitive function within the normal range were included in the study and underwent assessments of verbal memory. Hippocampal subfield volumes were determined based on T1-weighted magnetization-prepared rapid gradient echo (T1-MPRAGE) images, while group tractography and connectometry analyses were conducted using diffusion tensor images. Our findings indicated that the volumes of whole bilateral hippocampus, cornus ammonis (CA) 1, molecular layer, left subiculum, CA3, hippocampal tail, right CA4, and granule cell/molecular layers of the dentate gyrus (GC-ML-DG) were significantly smaller in the subclinical depression group than in the control group. In the subclinical depression group, verbal learning was positively correlated with the volumes of the CA1, GC-ML-DG, molecular layer, and whole hippocampus in the right hemisphere. The fractional anisotropy of the bilateral fornix was also significantly lower in the subclinical depression group and exhibited a positive correlation with verbal learning and recall in both groups. Our results suggest that hippocampal microstructure is disrupted and associated with memory in patients with subclinical depression.
Management of Cavernous Carotid Artery Aneurysms: A Retrospective Single-Center Experience
Authors: Fehrenbach Michael Karl , Dietel Eric , Wende Tim , Kasper Johannes , Sander Caroline , Wilhelmy Florian , Quaeschling Ulf , Meixensberger Juergen , Nestler Ulf
Published: March 2022

Objective: While cavernous carotid aneurysms can cause neurological symptoms, their often-uneventful natural course and the increasing options of intravascular aneurysm closure call for educated decision-making. However, evidence-based guidelines are missing. Here, we report 64 patients with cavernous carotid aneurysms, their respective therapeutic strategies, and follow-up. Methods: We included all patients with cavernous carotid aneurysms who presented to our clinic between 2014 and 2020 and recorded comorbidities (elevated blood pressure, diabetes mellitus, and nicotine consumption), PHASES score, aneurysm site, size and shape, therapeutic strategy, neurological deficits, and clinical follow-up. Results: The mean age of the 64 patients (86% female) was 53 years, the mean follow-up time was 3.8 years. A total of 22 patients suffered from cranial nerve deficit. Of these patients, 50% showed a relief of symptoms regardless of the therapy regime. We found no significant correlations between aneurysm size or PHASES score and the occurrence of neurological symptoms. Conclusion: If aneurysm specific symptoms persist over a longer period of time, relief is difficult to achieve despite aneurysm treatment. Patients should be advised by experts in neurovascular centers, weighing the possibility of an uneventful course against the risks of treatment. In this regard, more detailed prospective data is needed to improve individual patient counseling.
Tinnitus Perception in Light of a Parietal Operculo–Insular Involvement: A Review
Authors: Jaroszynski Chloé , Job Agnès , Jedynak Maciej , David Olivier , Delon-Martin Chantal
Published: March 2022

In tinnitus literature, researchers have increasingly been advocating for a clearer distinction between tinnitus perception and tinnitus-related distress. In non-bothersome tinnitus, the perception itself can be more specifically investigated: this has provided a body of evidence, based on resting-state and activation fMRI protocols, highlighting the involvement of regions outside the conventional auditory areas, such as the right parietal operculum. Here, we aim to conduct a review of available investigations of the human parietal operculo–insular subregions conducted at the microscopic, mesoscopic, and macroscopic scales arguing in favor of an auditory–somatosensory cross-talk. Both the previous literature and new results on functional connectivity derived from cortico–cortical evoked potentials show that these subregions present a dense tissue of interconnections and a strong connectivity with auditory and somatosensory areas in the healthy brain. Disrupted integration processes between these modalities may thus result in erroneous perceptions, such as tinnitus. More precisely, we highlight the role of a subregion of the right parietal operculum, known as OP3 according to the Jülich atlas, in the integration of auditory and somatosensory representation of the orofacial muscles in the healthy population. We further discuss how a dysfunction of these muscles could induce hyperactivity in the OP3. The evidence of direct electrical stimulation of this area eliciting auditory hallucinations further suggests its involvement in tinnitus perception. Finally, a small number of neuroimaging studies of therapeutic interventions for tinnitus provide additional evidence of right parietal operculum involvement.
The Role of Guilt and Empathy on Prosocial Behavior
Authors: Scaffidi Abbate Costanza , Misuraca Raffaella , Roccella Michele , Parisi Lucia , Vetri Luigi , Miceli Silvana
Published: March 2022

Research on the effects of guilt on interpersonal relationships has shown that guilt frequently motivates prosocial behavior in dyadic social situations. When multiple persons are involved, however, this emotion can be disadvantageous for other people in the social environment. Two experiments were carried out to examine the effect of guilt and empathy on prosocial behavior in a context in which more than two people are involved. Experiment 1 investigates whether, in three-person situations, guilt motivates prosocial behavior with beneficial effects for the victim of one’s actions but disadvantageous effects for the third individual. Participants were faced with a social dilemma in which they could choose to take action that would benefit themselves, the victim, or the other individual. The findings show that guilt produces disadvantageous side effects for the third individual person present without negatively affecting the transgressor’s interest. In Experiment 2, participants were faced with a social dilemma in which they could act to benefit themselves, the victim, or a third person for whom they were induced to feel empathic concern. Again, the results show that guilt generates advantages for the victim but, in this case, at the expense of the transgressor and not at the expense of the third person, for whom they were induced to feel empathic concern. Therefore, guilt and empathy seem to limit the transgressor’s interest. The theoretical implications are discussed.
Byzantine Churches in Albania: How Geometry and Architectural Composition Influence the Acoustics
Authors: Sukaj Silvana , Bevilacqua Antonella , Iannace Gino , Lombardi Ilaria , Parente Rosaria , Trematerra Amelia
Published: March 2022

The Byzantine churches built between the 7th and the 15th centuries represent an architectural typology that has been developed along with other architectural styles. The evolution of the interior organization, to be composed of only a single nave for the oldest architecture and then developed with additional lateral naves, is one of the multiple aspects that characterizes this building type, studied mainly from an architectural perspective. The variety of roof morphology, being flat, double slope, vaulted or domed, contributes to determining the overall acoustics. This paper deals with the analysis of the acoustic characteristics related to five Byzantine churches located in Albania, specifically in Berat and Cete. A comparison of the impulse response (IR) measured inside each church was given by analyzing the most appropriate acoustic parameters and in line with ISO 3382. The acoustic surveys were undertaken with a minimal furniture and without any audience. The results highlight small difficulties in terms of speech understanding, especially under a speech clarity index found to be below the optimal range limit. This shortfall is attributed to the geometry of the volumes and to the reflecting materials applied to the surfaces that facilitate the build-up of sound energy.
Synthesis and Mathematical Modelling of the Preparation Process of Nickel-Alumina Catalysts with Egg-Shell Structures for Syngas Production via Reforming of Clean Model Biogas
Authors: Latsiou Angeliki I. , Bereketidou Olga A. , Charisiou Nikolaos D. , Georgiadis Amvrosios G. , Avraam Dimitrios G. , Goula Maria A.
Published: March 2022

For the work presented herein nickel catalysts supported on γ-alumina extrudates (Ni/Al) with an egg-shell structure were prepared, using a modified Equilibrium Deposition Filtration (EDF) technique. Their performance was compared, for the biogas dry reforming reaction, with corresponding Ni/Al catalysts with a uniform structure, synthesized via the conventional wet impregnation method. The bulk and surface physicochemical characteristics of all final catalysts were determined using ICP-AES, N2 adsorption-desorption isotherms, XRD, SEM, and TEM. A theoretical model describing the impregnation process for the EDF extrudates, based on the Lee and Aris model, was also developed. It was concluded that following specific impregnation conditions, the egg-shell macro-distributions can be successfully predicted, in agreement with the experimental results. It was shown that the Ni/Al catalysts with an egg-shell structure had a higher H2 yield in comparison with the ones with a uniform structure. The difference in catalytic performance was attributed to the improved surface and structural properties of the egg-shell catalysts, resulting from the modified EDF technique used for their preparation.
Single-Atom Catalysts for the Electro-Reduction of CO2 to Syngas with a Tunable CO/H2 Ratio: A Review
Authors: Scarpa Davide , Sarno Maria
Published: March 2022

Nowadays, transition towards green chemistry is becoming imperative. In this scenario, an attractive perspective consists in the generation of CO through the electrochemical reduction of CO2 under ambient conditions. This approach allows storage of the electrical energy from intermittent renewable sources in the form of chemical bonds, and simultaneously reduces greenhouse gas emissions, giving carbon a second chance of life. However, most catalysts adopted for this process, i.e., noble metal-based nanoparticles, still have several issues (high costs, low current densities, high overpotentials), and in the view of generating syngas through co-electrolysis of H2O and CO2, do not enable a widely tunable CO/H2 ratio. Single-atom catalysts with N-doped carbon supports have been recently introduced to face these challenges. The following review aims to answer the demand for an extended and exhaustive analysis of the metal single-atom catalysts thus far explored for the electro-reduction of CO2 in aqueous electrolyte solution. Moreover, focus will be placed on the objective of generating a syngas with a tunable CO/H2 ratio. Eventually, the advantages of single-atom catalysts over their noble metal-based nano-sized counterparts will be identified along with future perspectives, also in the view of a rapid and feasible scaling-up.
The Osteocyte Stimulated by Wnt Agonist SKL2001 Is a Safe Osteogenic Niche Improving Bioactivities in a Polycaprolactone and Cell Integrated 3D Module
Authors: Liu Yangxi , Ruan Xiaojie , Li Jun , Wang Bo , Chen Jie , Wang Xiaofang , Wang Pengtao , Tu Xiaolin
Published: March 2022

Finding and constructing an osteogenic microenvironment similar to natural bone tissue has always been a frontier topic in orthopedics. We found that osteocytes are targeting cells controlling bone anabolism produced by PTH (JBMR 2017, PMID: 27704638), and osteocytes with activated Wnt signaling orchestrate bone formation and resorption (PNAS 2015, PMID: 25605937). However, methods for taking advantage of the leading role of osteocytes in bone regeneration remain unexplored. Herein, we found that the osteocytes with SKL2001-activated Wnt signaling could be an osteogenic microenvironment (SOOME) which upregulates the expression of bone transcription factor Runx2 and Bglap and promotes the differentiation of bone marrow stromal cell ST2 into osteoblasts. Interestingly, 60 μM SKL2001 treatment of osteocytic MLO-Y4 for 24 h maintained Wnt signaling activation for three days after removal, which was sufficient to induce osteoblast differentiation. Triptonide, a Wnt inhibitor, could eliminate this differentiation. Moreover, on day 5, the Wnt signaling naturally decreased to the level of the control group, indicating that this method of Wnt-signaling induction is safe to use. We quickly verified in vivo function of SOOME to a good proximation in 3D bioprinted modules composed of reciprocally printed polycaprolactone bundles (for support) and cell bundles (for bioactivity). In the cell bundles, SOOME stably supported the growth and development of ST2 cells, the 7-day survival rate was as high as 91.6%, and proliferation ability increased linearly. Similarly, SOOME greatly promoted ST2 differentiation and mineralization for 28 days. In addition, SOOME upregulated the expression of angiopoietin 1, promoted endothelial cell migration and angiogenesis, and increased node number and total length of tubes and branches. Finally, we found that the function of SOOME could be realized through the paracrine pathway. This study reveals that osteocytes with Wnt signaling activated by SKL2001 are a safe osteogenic microenvironment. Both SOOME itself and its cell-free culture supernatant can improve bioactivity for osteoblast differentiation, with composite scaffolds especially bearing application value.
Pyk2 Regulates MAMs and Mitochondrial Dynamics in Hippocampal Neurons
Authors: López-Molina Laura , Fernández-Irigoyen Joaquín , Cifuentes-Díaz Carmen , Alberch Jordi , Girault Jean-Antoine , Santamaría Enrique , Ginés Silvia , Giralt Albert
Published: March 2022

Pyk2 is a non-receptor tyrosine kinase enriched in hippocampal neurons, which can be activated by calcium-dependent mechanisms. In neurons, Pyk2 is mostly localised in the cytosol and dendritic shafts but can translocate to spines and/or to the nucleus. Here, we explore the function of a new localisation of Pyk2 in mitochondria-associated membranes (MAMs), a subdomain of ER-mitochondria surface that acts as a signalling hub in calcium regulation. To test the role of Pyk2 in MAMs’ calcium transport, we used full Pyk2 knockout mice (Pyk2−/−) for in vivo and in vitro studies. Here we report that Pyk2−/− hippocampal neurons present increased ER-mitochondrial contacts along with defective calcium homeostasis. We also show how the absence of Pyk2 modulates mitochondrial dynamics and morphology. Taken all together, our results point out that Pyk2 could be highly relevant in the modulation of ER-mitochondria calcium efflux, affecting in turn mitochondrial function.
Extracellular Oxidative Stress Markers in COVID-19 Patients with Diabetes as Co-Morbidity
Authors: Kumar Devika Sanil , Hanumanram Gowtham , Suthakaran Prasanna Karthik , Mohanan Jagadeesan , Nair Lal Devayani Vasudevan , Rajendran Kannan
Published: March 2022

COVID-19 patients have a higher risk of developing inflammatory responses associated with serious and even fatal respiratory diseases. The role of oxidative stress in exacerbating manifestations in COVID-19 pathogenesis is under-reported.This study aimed touseserum levels of superoxide dismutase (SOD3) and glutathione-S-transferase (GSTp1) by ELISA, zinc (ErbaChem5), ferritin and free iron (VitrosChemistry, Ortho Clinical Diagnosis, Raritan, NJ, USA) at the first encounter of randomly selected RT-PCR-positive COVID-19 patients, for assessing disease severity. The parameters which helped in identifying the severity, leading to poor prognosis, were neutrophil:lymphocyte higher than 4, high CRP, low SOD3 values and high GSTp1 values, and diabetes mellitus as a co-morbidity. Higher zinc levels correlated with high GSTp1 and low SOD3, indicating the protective effect of zinc on ROS. The increased high GSTp1 shows an anticipated protective biochemical response, to mitigate the low SOD3 values due to ROS consumption. Decreased SOD3 levels indicate a state of high oxidative stress at cellular levels, and an anticipated increase in GSTp1 levels points to the pathophysiological bases of increasing severity with age, sex, and co-morbidities, such asdiabetes. High levels of initial GSTp1 and zinc levels possibly offer protection to redox reactions at the cellular level in severe COVID-19 infection, preventing deterioration.
Circadian and Sleep Modulation of Dreaming in Women with Major Depression
Authors: Birchler-Pedross Angelina , Frey Sylvia , Cajochen Christian , Chellappa Sarah L.
Published: March 2022

Growing evidence indicates an association between reduced dream recall and depressive symptomatology. Here, we tested the prediction that reduced dream recall in individuals experiencing major depressive disorder (MDD) is due to alterations in circadian and sleep processes. Nine young healthy women (20–31 years) and eight young unmedicated women (20–31 years) diagnosed with MDD underwent a 40 h multiple nap protocol with ten alternating cycles of 150 min wake/75 min sleep under a stringently controlled circadian laboratory protocol. After each nap, we assessed dream recall, number of dreams and dream emotional load using the Sleep Mentation Questionnaire. Dream recall and the number of dreams did not significantly differ between groups (pFDR > 0.1). However, there was a significant difference for the dream emotional load (interaction of “Group” vs. “Time”, pFDR = 0.01). Women with MDD had a two-fold higher (negative) emotional load as compared to healthy control women, particularly after naps during the circadian night (between ~22:00 h and ~05:00 h; Tukey–Kramer test, p = 0.009). Furthermore, higher (negative) dream emotional load was associated with impaired mood levels in both groups (R2 = 0.71; p < 0.001). Our findings suggest that the circadian and sleep modulation of dreaming may remain intact in unmedicated young women experiencing MDD.
Improved Cycling Performance and High Rate Capacity of LiNi0.8Co0.1Mn0.1O2 Cathode Achieved by Al(PO3)3 Modification via Dry Coating Ball Milling
Authors: Wang Feng , Luo Yikuan , Liu Peng , Balogun Muhammad-Sadeeq , Deng Jianqiu , Wang Zhongmin
Published: March 2022

LiNi0.8Co0.1Mn0.1O2 (NCM811) has attracted extensive attention as a promising cathode of lithium-ion batteries (LIBs) in next-generation electric vehicles, as the NCM811 sample possesses a high energy density and a price advantage. In this work, NCM811 was modified with an Al(PO3)3 precursor using the dry ball milling method followed by heat treatment to enable commercial development both at room temperature and a higher temperature. Compared with the unmodified NCM811 sample with the capacity retention of 68.70%, after Al(PO3)3 modification, the NCM811 sample heated to 500 °C exhibited a super capacity retention ratio of 93.88% after 200 charging–discharging cycles with the initial discharge capacity of 178.1 mAh g−1 at 1 C. Additionally, after Al(PO3)3 modification, the NCM811 sample heated to 500 °C showed much improved rate performance compared to bare NCM811 at the current density of 5 C. The enhanced electrochemical performance after cycling was due to the decreased charge transfer resistance and increased Li+ transmission, which were confirmed via electrochemical impedance spectra (EIS). The NCM electrodes showed improved structural stability as layered structures after Al(PO3)3 modification, consistent with the improved cycling performance. This work revealed that LiNi0.8Co0.1Mn0.1O2 material with phosphide coating can be constructed using a simple ball milling method, which is feasible for obtaining high-performance electrode materials.
New In Silico Insights into the Application of (Hydroxy)Chloroquine with Macrolide Antibiotic Co-Crystals against the SARS-CoV-2 Virus
Authors: de Castro Alexandre A. , Assis Letícia C. , da Cunha Elaine F. F. , Ramalho Teodorico C. , La Porta Felipe A.
Published: March 2022

In this in silico study, different pharmaceutical co-crystals based on (hydroxy)chloroquine with macrolide antibiotics (azithromycin, clarithromycin, or erythromycin A) were analyzed for the first time. These findings present a new molecular perspective and therefore suggest that the combination of (hydroxy)chloroquine/azithromycin, in the stoichiometric ratio of 1:1, as model co-crystal systems has less toxicity and is the most effective for inhibiting the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Reduction in the Photoluminescence Intensity Caused by Ultrathin GaN Quantum Barriers in InGaN/GaN Multiple Quantum Wells
Authors: Liu Wei , Liang Feng , Zhao Degang , Yang Jing , Chen Ping , Liu Zongshun
Published: March 2022

The optical properties of InGaN/GaN violet light-emitting multiple quantum wells with different thicknesses of GaN quantum barriers are investigated experimentally. When the barrier thickness decreases from 20 to 10 nm, the photoluminescence intensity at room temperature increases, which can be attributed to the reduced polarization field in the thin-barrier sample. However, with a further reduction in the thickness to 5 nm, the sample’s luminescence intensity decreases significantly. It is found that the strong nonradiative loss process induced by the deteriorated crystal quality and the quantum-tunneling-assisted leakage of carriers may jointly contribute to the enhanced nonradiative loss of photogenerated electrons and holes, leading to a significant reduction in photoluminescence intensity of the sample with nanoscale ultrathin GaN quantum barriers.
Low Platelet Count Predicts Reduced Survival in Potentially Resectable Hepatocellular Carcinoma
Authors: Schrecker Christopher , Waidmann Oliver , El Youzouri Hanan , Trojan Jörg , Schnitzbauer Andreas Anton , Bechstein Wolf Otto , Zeuzem Stefan , Koch Christine
Published: March 2022

The prognostic role of platelet count in hepatocellular carcinoma (HCC) remains unclear, and in fact both thrombocytopenia and thrombocytosis are reported as predictors of unfavourable outcomes. This study aimed to clarify the prognostic value of preoperative platelet count in potentially resectable HCC. We retrospectively reviewed 128 patients who underwent hepatic resection for HCC at a tertiary academic centre (2007–2019). Patient data were modelled by regression analysis, and platelet count was treated as a continuous variable. 89 patients had BCLC 0/A tumours and 39 had BCLC B tumours. Platelet count was higher in patients with larger tumours and lower in patients with higher MELD scores, advanced fibrosis, and portal hypertension (p < 0.001 for all listed variables). After adjusting for BCLC stage and tumour diameter, low platelet count associated with reduced overall survival (hazard ratio 1.25 per 50/nL decrease in platelet count, 95% confidence interval (CI) 1.02–1.53, p = 0.034) and increased perioperative mortality (odds ratio 1.96 per 50/nL decrease in platelet count, 95% CI 1.19–3.53, p = 0.014). Overall, low platelet count correlates with increased liver disease severity, inferior survival, and excess perioperative mortality in resectable HCC. These insights might be applied in clinical practice to better select patients for resection.
Comment on Li et al. BDP1 Variants I1264M and V1347M Significantly Associated with Clinical Outcomes of Pediatric Neuroblastoma Patients Imply a New Prognostic Biomarker: A 121-Patient Cancer Genome Study. Diagnostics 2021, 11, 2364
Authors: Schramm Laura
Published: March 2022

In 2021, Li et al. published an article on BDP1 variants and implications in pediatric neuroblastoma patients [...]
Use of U-Net Convolutional Neural Networks for Automated Segmentation of Fecal Material for Objective Evaluation of Bowel Preparation Quality in Colonoscopy
Authors: Wang Yen-Po , Jheng Ying-Chun , Sung Kuang-Yi , Lin Hung-En , Hsin I-Fang , Chen Ping-Hsien , Chu Yuan-Chia , Lu David , Wang Yuan-Jen , Hou Ming-Chih , Lee Fa-Yauh , Lu Ching-Liang
Published: March 2022

Background: Adequate bowel cleansing is important for colonoscopy performance evaluation. Current bowel cleansing evaluation scales are subjective, with a wide variation in consistency among physicians and low reported rates of accuracy. We aim to use machine learning to develop a fully automatic segmentation method for the objective evaluation of the adequacy of colon preparation. Methods: Colonoscopy videos were retrieved from a video data cohort and transferred to qualified images, which were randomly divided into training, validation, and verification datasets. The fecal residue was manually segmented. A deep learning model based on the U-Net convolutional network architecture was developed to perform automatic segmentation. The performance of the automatic segmentation was evaluated on the overlap area with the manual segmentation. Results: A total of 10,118 qualified images from 119 videos were obtained. The model averaged 0.3634 s to segmentate one image automatically. The models produced a strong high-overlap area with manual segmentation, with 94.7% ± 0.67% of that area predicted by our AI model, which correlated well with the area measured manually (r = 0.915, p < 0.001). The AI system can be applied in real-time qualitatively and quantitatively. Conclusions: We established a fully automatic segmentation method to rapidly and accurately mark the fecal residue-coated mucosa for the objective evaluation of colon preparation.
Modeling of Diesel Engine Fuel Systems Reliability When Operating on Biofuels
Authors: Zhuravel Dmytro , Samoichuk Kyrylo , Petrychenko Serhii , Bondar Andrii , Hutsol Taras , Kuboń Maciej , Niemiec Marcin , Mykhailova Lyudmyla , Gródek-Szostak Zofia , Sorokin Dmytro
Published: March 2022

One of the main trends in the development of modern engine building is the use of biodiesel fuel, which can lead to a decrease in the reliability of engines that are not adapted to it. However, at present there is no general method for determining the reliability of fuel systems of internal combustion engines. In this paper, a reliability model of engine fuel systems when operating on biofuel has been developed. Comprehensive indicators for assessing the reliability of diesel engine fuel systems are the coefficient of readiness and technical use. The availability factor of the fuel system when operating on biodiesel fuel without the replacement of structural materials was 0.66, while with the replacement it was 0.71, and the coefficient of technical utilization without replacement of materials was 0.36, and with the replacement of 0.4. Recommendations are given to improve the reliability of the engine fuel system components. The resulting model allows for complex comparisons of the effectiveness of various ways to improve the reliability of engines running on biodiesel fuel.
Catalytic Removal of NOx on Ceramic Foam-Supported ZnO and TiO2 Nanorods Ornamented with W and V Oxides
Authors: Kapkowski Maciej , Siudyga Tomasz , Bartczak Piotr , Zubko Maciej , Sitko Rafal , Szade Jacek , Balin Katarzyna , Witkowski Bartłomiej S. , Ożga Monika , Pietruszka Rafał , Godlewski Marek , Polanski Jaroslaw
Published: March 2022

Energy consumption steadily increases and energy production is associated with many environmental risks, e.g., generating the largest share of greenhouse gas emissions. The primary gas pollution concern is CO2, CH4, and nitrogen oxides (NOx). Environmental catalysis plays a pivotal role in NOx mitigation (DeNOx). This study investigated, for the first time, a collection of ceramic foams as potential catalyst support for selective catalytic NOx reduction (SCR). Ceramic foams could be an attractive support option for NOx removal. However, we should functionalize the surface of raw foams for such applications. A library of ceramic SiC, Al2O3, and ZrO2 foams ornamented with nanorod ZnO and TiO2 as W and V oxide support was obtained for the first time. We characterized the surface layer coating structure using the XPS, XRF and SEM, and TEM microscopy to optimize the W to V molar ratio and examine NO2 mitigation as the SCR model, which was tested only very rarely. Comparing TiO2 and ZnO systems reveals that the SCR conversion on ZnO appeared superior vs. the conversion on TiO2, while the SiC-supported catalysts were less efficient than Al2O3 and ZrO2-supported catalysts. The energy bands in optical spectra correlate with the observed activity rank.
Improvements in the Water Retention Characteristics and Thermophysical Parameters of Backfill Material in Ground Source Heat Pumps by a Molecular Sieve
Authors: Luo Tingting , Pei Peng , Chen Yixia , Hao Dingyi , Wang Chen
Published: March 2022

The thermophysical properties of backfill material (BM) in a heat exchange borehole significantly influence the heat exchange effect of ground source heat pumps (GSHPs). Several treatments such as compaction and adding bentonite, cement, and fine sands are often used to improve the thermophysical properties. In this study, a 3A molecular sieve (3A-MS), a type of porous material, was added to the BM to enhance its water maintaining capacity. Three types of backfill materials with different additive contents, named as BM-0, BM-1, and BM-2, were examined. The variation of the BM properties such as the soil–water characteristic curve (SWCC), thermal conductivity, specific heat capacity, and thermal diffusivity with the groundwater content were investigated through a series of experiments and simulations. A scanning electron microscope (SEM), an energy dispersive spectrometer (EDS), and the BET method for specific surface area pore size analysis were used to characterize the material. The results indicated that the specific heat capacity improved with the water content whereas the thermal conductivity and thermal diffusivity decreased with the water content. The variation of the buried pipe outlet temperature with the change of the thermal physical parameters of the BM were researched by a numerical simulation and theoretical calculations; the results showed that BM-2 could raise the heat transfer rate per meter by 45.9% in summer and 118.4% in winter compared with the backfill materials without groundwater (NW). The research results provide theoretical support for the improvement of BM for ground source heat pump projects where abundant groundwater is available.
Modeling Analysis of SOFC System Oriented to Working Condition Identification
Authors: Wu Xiao-Long , Zhang Hong , Liu Hongli , Xu Yuan-Wu , Peng Jingxuan , Xia Zhiping , Wang Yongan
Published: March 2022

Solid oxide fuel cell (SOFC) generation system is an important equipment to realize “carbon neutralization”. In SOFC system, a fault will cause changes in working conditions, which is difficult to detect early and find the reason due to the high temperature and seal environment. Therefore, the mechanistic model is a feasible way to find the reasons for the change of system working conditions. In this paper, based on the first law of thermodynamics, the system model of SOFC is built under multiple working conditions, and the influence of stack, afterburner, heat exchanger, and reformer fault is studied on the thermoelectric characteristics and efficiency of the system. The results show that with the introduction of these fault mechanistic models, the dynamic response characteristics of SOFC system under multiple working conditions can be obtained by tracking the key performance parameters qualitatively. The work of this paper is helpful for the guidance of the fault diagnosis of SOFC system in the future.
Predicting the Compressibility Factor of Natural Gas by Using Statistical Modeling and Neural Network
Authors: Ghanem Alaa , Gouda Mohammed F. , Alharthy Rima D. , Desouky Saad M.
Published: March 2022

Simulating the phase behavior of a reservoir fluid requires the determination of many parameters, such as gas–oil ratio and formation volume factor. The determination of such parameters requires knowledge of the critical properties and compressibility factor (Z factor). There are many techniques to determine the compressibility factor, such as experimental pressure, volume, and temperature (PVT) tests, empirical correlations, and artificial intelligence approaches. In this work, two different models based on statistical regression and multi-layer-feedforward neural network (MLFN) were developed to predict the Z factor of natural gas by utilizing the experimental data of 1079 samples with a wide range of pseudo-reduced pressure (0.12–25.8) and pseudo reduced temperature (1.3–2.4). The statistical regression model was proposed and trained in R using the “rjags” package and Markov chain Monte Carlo simulation, while the multi-layer-feedforward neural network model was postulated and trained using the “neural net” package. The neural network consists of one input layer with two anodes, three hidden layers, and one output layer. The input parameters are the ratio of pseudo-reduced pressure and the pseudo-reduced temperature of the natural hydrocarbon gas, while the output is the Z factor. The proposed statistical and MLFN models showed a positive correlation between the actual and predicted values of the Z factor, with a correlation coefficient of 0.967 and 0.979, respectively. The results from the present study show that the MLFN can lead to accurate and reliable prediction of the natural gas compressibility factor.
Applicability of Transient Electromagnetic Surveys to Permafrost Imaging in Arctic West Siberia
Authors: Buddo Igor , Sharlov Maxim , Shelokhov Ivan , Misyurkeeva Natalya , Seminsky Igor , Selyaev Vasily , Agafonov Yury
Published: March 2022

Detection of faults and other zones of weakness in shallow permafrost to a few hundreds of meters is extremely important for ensuring the safety during the production and transportation of fuels (oil and gas). The construction of line facilities (power lines and pipelines) should be preceded by detailed surveys in order to localize major areas of potential hazard. Furthermore, reliable geophysical methods are necessary for exploration of gas hydrates. This research aims at proving that induction-based electromagnetic surveys are applicable for permafrost studies and at finding new evidence for the similarity and difference of the permafrost structure in different regions of Northern Siberia. TEM curves are collected in several regions of Northern Siberia with continuous, mostly continuous, and discontinuous permafrost. Transient electromagnetic (TEM) surveys performed in the Russian Arctic image the permafrost structure to a depth of 500 m. The data are acquired with telemetric systems that allow varying the survey design and loop configuration. Advanced processing tools are used to provide geologically essential information from late-time records, while optimized inversion algorithms are applied to obtain high-quality layered resistivity models. The resulting geoelectric models reveal evident variations in the thickness of highly resistive frozen rocks and the presence of unfrozen patches. The induction surveys, which require no galvanic contact with the earth and no grounding, are inferred to be best suitable for imaging the frozen shallow subsurface. The TEM-based resistivity patterns clearly resolve the permafrost base, as well as the contours of unfrozen zones (taliks), lenses of saline water (cryopegs), gas hydrates, and frost heaving features. The reported results can make basis for the choice of geophysical methods for permafrost studies in such harsh conditions as the Russian Arctic. Furthermore, the presented resistivity patterns can make reference for future studies of permafrost in Northern Siberia.
The Resilient Smart City Model–Proposal for Polish Cities
Authors: Baran Małgorzata , Kłos Monika , Chodorek Monika , Marchlewska-Patyk Karolina
Published: March 2022

The smart city (SC) concept is currently one of the leading ideas in the field of management. It has also become important for Polish cities in terms of sustainable development. Therefore, it is important to determine the priority in urban development; and the areas that need investment to make cities smart. Based on the literature review, it can be stated that there are many available concepts of SCs since the level of development of many cities in the world is different and the priorities of the developed areas vary. The identified SC management models present common parts, resulting from the defining basis, as well as additional aspects important for an SC, stemming from their specificity and the approach of their managers. Thus, the aim of the article is to propose the author’s model of resilient smart city development in Poland. To achieve the objective, a systematic literature review was applied, and the analysis of existing SC management models in the world was carried out in order to identify these models components and documents profiling SC strategies. These areas should be the focus of managers when creating city development strategies in Poland.
A Systematic Review of the Behavioural Changes and Physiological Adjustments of Elasmobranchs and Teleost’s to Ocean Acidification with a Focus on Sharks
Authors: Zemah-Shamir Ziv , Zemah-Shamir Shiri , Scheinin Aviad , Tchernov Dan , Lazebnik Teddy , Gal Gideon
Published: March 2022

In recent years, much attention has been focused on the impact of climate change, particularly via ocean acidification (OA), on marine organisms. Studying the impact of OA on long-living organisms, such as sharks, is especially challenging. When the ocean waters absorb anthropogenic carbon dioxide (CO2), slow-growing shark species with long generation times may be subjected to stress, leading to a decrease in functionality. Our goal was to examine the behavioral and physiological responses of sharks to OA and the possible impacts on their fitness and resilience. We conducted a systematic review in line with PRISMA-Analyses, of previously reported scientific experiments. We found that most studies used CO2 partial pressures (pCO2) that reflect representative concentration pathways for the year 2100 (e.g., pH ~7.8, pCO2 ~1000 μatm). Since there is a considerable knowledge gap on the effect of OA on sharks, we utilized existing data on bony fish to synthesize the available knowledge. Given the similarities between the behaviors and physiology of these two superclasses’ to changes in CO2 and pH levels, there is merit in including the available information on bony fish as well. Several studies indicated a decrease in shark fitness in relation to increased OA and CO2 levels. However, the decrease was species-specific and influenced by the intensity of the change in atmospheric CO2 concentration and other anthropogenic and environmental factors (e.g., fishing, temperature). Most studies involved only limited exposure to future environmental conditions and were conducted on benthic shark species studied in the laboratory rather than on apex predator species. While knowledge gaps exist, and more research is required, we conclude that anthropogenic factors are likely contributing to shark species’ vulnerability worldwide. However, the impact of OA on the long-term stability of shark populations is not unequivocal.
Fractal Nature Bridge between Neural Networks and Graph Theory Approach within Material Structure Characterization
Authors: Randjelovic Branislav M. , Mitic Vojislav V. , Ribar Srdjan , Milosevic Dusan M. , Lazovic Goran , Fecht Hans J. , Vlahovic Branislav
Published: March 2022

Many recently published research papers examine the representation of nanostructures and biomimetic materials, especially using mathematical methods. For this purpose, it is important that the mathematical method is simple and powerful. Theory of fractals, artificial neural networks and graph theory are most commonly used in such papers. These methods are useful tools for applying mathematics in nanostructures, especially given the diversity of the methods, as well as their compatibility and complementarity. The purpose of this paper is to provide an overview of existing results in the field of electrochemical and magnetic nanostructures parameter modeling by applying the three methods that are “easy to use”: theory of fractals, artificial neural networks and graph theory. We also give some new conclusions about applicability, advantages and disadvantages in various different circumstances.
Old English Enigmatic Poems and Their Reception in Early Scholarship and Supernatural Fiction
Authors: Murphy Patrick Joseph
Published: March 2022

The scholarly reception history of the Old English riddles and adjacent “enigmatic poems” of the Exeter Book reveals a long process of creating intelligibility and order out of a complicated and obscure manuscript context. Understanding this history of reception allows us to see the influence of Old English poetry on modern creative medievalism, including the unexpected influence of medieval “enigmatic” poetry on the modern genre of supernatural fiction. Specifically, it is argued that the scholarly reception of folios 122v–123v of the Exeter Anthology was instrumental in inspiring one of the acknowledged classic ghost stories of the twentieth century, M.R. James’s “Oh, Whistle, and I’ll Come to You, My Lad”.
Detection of Red Blood Cell Membrane Proteins in Myelodysplastic Syndromes Using Eosin-5-Maleimide (EMA) Staining by Flow Cytometry
Authors: Uman Navavee , Kobbuaklee Sirorat , Kansuwan Patsita , Watanaboonyongcharoen Phandee , Polprasert Chantana
Published: March 2022

Background: Eosin-5-Maleimide (EMA)-based flow cytometry binds to red blood cell (RBC) membrane-associated proteins which can be used to detect red blood cell (RBC) membrane disorders. Myelodysplastic syndromes (MDS) are stem cell disorders resulting in ineffective hematopoiesis which is commonly present with anemia and erythroid dysplasia. Objectives: We aimed to study RBC membrane defects in MDS using flow cytometry for EMA staining. Methods: We enrolled anemic patients who were diagnosed with low-risk MDS (R-IPSS score ≤ 3.5), RBC membrane disorders [hereditary spherocytosis (HS) and Southeast Asian ovalocytosis (SAO)], and normal controls. Complete blood count (CBC) and flow cytometry for EMA staining were performed. Results: There were 16 cases of low-risk MDS, 6 cases of RBC membrane disorders, and 15 control cases. Mean fluorescence intensity (MFI) of EMA binding test in the RBC membrane disorders was significantly lower than controls (17.6 vs. 24.3, p < 0.001), but the EMA binding test in the low-risk MDS was not significantly different than the controls (26.5 vs. 24.3, p = 0.08). Conclusion: the RBC membrane defect in low-risk MDS was not demonstrated as having detection ability using EMA binding test with flow cytometry.
Correction: Haddad et al. Reviving Alexander Calder’s Man-Eater with Pennants: A Technical Examination of the Original Paint Palette. Heritage 2021, 4, 1920–1937
Authors: Haddad Abed , Randall Megan , Zycherman Lynda , Martins Ana
Published: March 2022

The authors wish to make the following corrections to this paper [...]
Key Role of Astrocytes in Postnatal Brain and Retinal Angiogenesis
Authors: Puebla Mariela , Tapia Pablo J. , Espinoza Hilda
Published: March 2022

Angiogenesis is a key process in various physiological and pathological conditions in the nervous system and in the retina during postnatal life. Although an increasing number of studies have addressed the role of endothelial cells in this event, the astrocytes contribution in angiogenesis has received less attention. This review is focused on the role of astrocytes as a scaffold and in the stabilization of the new blood vessels, through different molecules release, which can modulate the angiogenesis process in the brain and in the retina. Further, differences in the astrocytes phenotype are addressed in glioblastoma, one of the most devastating types of brain cancer, in order to provide potential targets involved in the cross signaling between endothelial cells, astrocytes and glioma cells, that mediate tumor progression and pathological angiogenesis. Given the relevance of astrocytes in angiogenesis in physiological and pathological conditions, future studies are required to better understand the interrelation between endothelial and astrocyte signaling pathways during this process.