bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomarkers of Glyco-Metabolic Control in Hemodialysis Patients: Glycated Hemoglobin vs. Glycated Albumin
Authors: Martino Francesca Gabriela , Vitillo Marina , Pieri Massimo , Marrone Giulia , Gangeri Fabio , Ansali Ferruccio , Dessì Mariarita , Bernardini Sergio , Di Daniele Nicola , Noce Annalisa
Published: July 2021

Background and Objectives: Glycated hemoglobin (HbA1c) dosage is considered the gold standard in glycol-metabolic monitoring, but it presents limits, which can underestimate the glycemia trend. In this regard, it was introduced the glycated albumin (GA). The aim of the study is to verify the predictivity of the GA compared to HbA1c in identifying glyco-metabolic alterations in non-diabetic and diabetic hemodialysis (HD) patients. Materials and Methods: For this purpose, we conducted a multicenter study involving one analysis laboratory and six dialysis centers in the Lazio region (Rome, Italy). Both diabetic and non-diabetic HD patients represent the study population, and the protocol included five time points. Results: The analyzed data highlighted the ability of GA to predict changes in glycemic metabolism in HD patients, and GA values are not significantly influenced, like HbA1c, by dialysis therapy itself and by comorbidities of the uremic state, such as normochromic and normocytic anemia. Thus, GA seems to reflect early glyco-metabolic alterations, both in patients with a previous diagnosis of diabetes and in subjects without diabetes mellitus. As part of this study, we analyzed two HD patients (one diabetic and one non-diabetic) in which GA was more predictive of glycol-metabolic alterations compared to HbA1c. Our study confirms the need to compare classical biomarkers used for the monitoring of glyco-metabolic alterations with new ones, likely more reliable and effective in specific subgroups of patients in which the classic biomarkers can be influenced by the preexisting pathological conditions. Conclusions: In conclusion, our evidence highlights that in uremic patients, GA shows a better ability to predict glyco-metabolic alterations allowing both an earlier diagnosis of DM and a prompt modulation of the hypoglycemic therapy, thus improving the clinical management of these patients.
Deciphering Antibody Responses to Orthonairoviruses in Ruminants
Authors: Hartlaub Julia , Keller Markus , Groschup Martin H.
Published: July 2021

Antibody cross-reactivities between related viruses are common diagnostic challenges, resulting in reduced diagnostic specificities and sensitivities. In this study, antibody cross-reactions between neglected members of the genus Orthonairovirus—Hazara (HAZV), Dugbe (DUGV), and Nairobi sheep disease orthonairovirus (NSDV)—were investigated. Mono-specific ovine and bovine sera following experimental infections as well immunization trials with HAZV, DUGV, and NSDV were tested in homologous and heterologous virus-specific assays, namely indirect ELISAs based on recombinant N protein, indirect immunofluorescence assays (iIFA), and two neutralization test formats (plaque reduction neutralization test (PRNT) and micro-virus neutralization test (mVNT)). The highest specificities were achieved with the ELISAs, followed by the mVNT, iIFA, and PRNT. Cross-reactivities were mainly observed within the Nairobi sheep disease serogroup–but surprisingly, HAZV antibodies in PRNT did also neutralize NSDV and DUGV. In conclusion, we recommend ELISAs and mVNTs for a discriminative diagnostic approach to differentiate between these antibodies. NSDV antisera were also used in serological assays for the detection of antibodies against the human pathogen Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV). Interestingly, all CCHFV ELISAs (In-house and commercial) achieved high diagnostic specificities, whereas significant cross-reactivities were observed in a CCHFV iIFA. Previously, similar results were obtained when analyzing the HAZV and DUGV antisera.
The Role of Streptococcus spp. in Bovine Mastitis
Authors: Kabelitz Tina , Aubry Etienne , van Vorst Kira , Amon Thomas , Fulde Marcus
Published: July 2021

The Streptococcus genus belongs to one of the major pathogen groups inducing bovine mastitis. In the dairy industry, mastitis is the most common and costly disease. It not only negatively impacts economic profit due to milk losses and therapy costs, but it is an important animal health and welfare issue as well. This review describes a classification, reservoirs, and frequencies of the most relevant Streptococcus species inducing bovine mastitis (S. agalactiae, S. dysgalactiae and S. uberis). Host and environmental factors influencing mastitis susceptibility and infection rates will be discussed, because it has been indicated that Streptococcus herd prevalence is much higher than mastitis rates. After infection, we report the sequence of cow immune reactions and differences in virulence factors of the main Streptococcus species. Different mastitis detection techniques together with possible conventional and alternative therapies are described. The standard approach treating streptococcal mastitis is the application of ß-lactam antibiotics. In streptococci, increased antimicrobial resistance rates were identified against enrofloxacin, tetracycline, and erythromycin. At the end, control and prevention measures will be considered, including vaccination, hygiene plan, and further interventions. It is the aim of this review to estimate the contribution and to provide detailed knowledge about the role of the Streptococcus genus in bovine mastitis.
Friends with Benefits: An Inside Look of Periodontal Microbes’ Interactions Using Fluorescence In Situ Hybridization—Scoping Review
Authors: Esteves Guilherme Melo , Pereira José António , Azevedo Nuno Filipe , Azevedo Andreia Sofia , Mendes Luzia
Published: July 2021

Fluorescence in situ hybridization (FISH) has proven to be particularly useful to describe the microbial composition and spatial organization of mixed microbial infections, as it happens in periodontitis. This scoping review aims to identify and map all the documented interactions between microbes in periodontal pockets by the FISH technique. Three electronic sources of evidence were consulted in search of suitable articles up to 7 November 2020: MEDLINE (via PubMed), Scopus (Elsevier: Amsterdam, The Netherlands), and Web of Science (Clarivate Analytics: Philadelphia, PA, USA) online databases. Studies that showed ex vivo and in situ interactions between, at least, two microorganisms were found eligible. Ten papers were included. Layered or radially ordered multiple-taxon structures are the most common form of consortium. Strict or facultative anaerobic microorganisms are mostly found in the interior and the deepest portions of the structures, while aerobic microorganisms are mostly found on the periphery. We present a model of the microbial spatial organization in sub- and supragingival biofilms, as well as how the documented interactions can shape the biofilm formation. Despite the already acquired knowledge, available evidence regarding the structural composition and interactions of microorganisms within dental biofilms is incomplete and large-scale studies are needed.
How to Handle CT-Guided Abscess Drainages in Microbiological Analyses? Sterile Vials vs. Blood Culture Bottles for Transport and Processing
Authors: Skusa Romy , Skusa Christopher , Wohlfarth Moritz , Hahn Andreas , Frickmann Hagen , Weber Marc-André , Podbielski Andreas , Warnke Philipp
Published: July 2021

The aim of this investigation was to compare microbiological analyses of 100 computed tomography-guided drainages from infectious foci (thoracic, abdominal, musculoskeletal), transported and analyzed by two widely established techniques, that are (i) sterile vials or (ii) inoculated blood culture bottles. The mean number of detected microorganisms from blood culture (aerobic/anaerobic) or conventional method (sterile vial, solid and broth media) per specimen were comparable with 1.29 and 1.41, respectively (p = 1.0). The conventional method showed a trend towards shorter time-to-result (median 28.62 h) in comparison to blood culture incubation (median 43.55 h) (p = 0.0722). Of note, detection of anaerobes (13% vs. 36%) and the number of detected microorganisms in polymicrobial infections (2.76 vs. 3.26) differed significantly with an advantage towards conventional techniques (p = 0.0015; p = 0.035), especially in abdominal aspirations. Despite substantially overlapping results from both techniques, the conventional approach includes some benefits which justify its role as standard approach.
Multidisciplinary Team-Based Palliative Care for Heart Failure and Food Intake at the End of Life
Authors: Shibata Tatsuhiro , Mawatari Kazutoshi , Nakashima Naoko , Shimozono Koutatsu , Ushijima Kouko , Yamaji Yumiko , Tetsuka Kumi , Murakami Miki , Okabe Kouta , Yanai Toshiyuki , Nohara Shoichiro , Takahashi Jinya , Aoki Hiroki , Yasukawa Hideo , Fukumoto Yoshihiro
Published: July 2021

Traditionally, patients with end-stage heart failure (HF) have rarely been involved in end-of-life care (EOLC) discussions in Japan. The purpose of this study was to examine the impact of HF-specific palliative care team (HF-PCT) activities on EOLC discussions with patients, HF therapy and care, and food intake at the end of life. We retrospectively analyzed 52 consecutive patients with HF (mean age, 70 ± 15 years; 42% female) who died at our hospital between May 2013 and July 2020 and divided them into two groups: before (Era 1, n = 19) and after (Era 2, n = 33) the initiation of HF-PCT activities in June 2015. Compared to Era 1, Era 2 showed a decrease in invasive procedures, an increase in opioid and non-intubating sedative use for symptom relief, improved quality of meals at the end of life, and an increase in participation in EOLC discussions. The administration of artificial nutrition in the final three days was associated with non-ischemic cardiomyopathy etiology, the number of previous hospitalizations for HF, and multidisciplinary EOLC discussion support. HF-PCT activities may provide an opportunity to discuss EOLC with patients, reduce the burden of physical and psychological symptoms, and shift the goals of end-of-life nutritional intake to ensure comfort and quality of life.
Effects of Perceived Food Store Environment on Malnutrition and Frailty among the Food-Insecure Elderly in a Metropolitan City
Authors: Kim Yu-Mi , Yang Narae , Kim Kirang
Published: July 2021

This study aimed to identify food environment factors in the local community that could affect the levels of nutritional status and frailty in 372 older adults (at least 65 years old) experiencing food insecurity and enrolled in the integrated Community Health Promotion Program (CHPP) in two districts of Seoul. The local food environment was assessed using perceived food store accessibility questionnaires. In order to quantify nutrient intake, the 24-h recall method was applied. Malnutrition was measured using the Mini Nutritional Assessment tool, while frailty was assessed using the Frailty Measurement Questionnaire developed for the CHPP. Malnourished or frail elderly adults commonly had a lower intake of cereals and potatoes, meats, and vegetables than those who were not, and their resulting intake levels of energy, protein, iron, and vitamin groups were also significantly lower (all p-values < 0.05). Among the local community food store environment factors, the sufficiency of food stores (odds ratio (OR) = 1.988, 95% confidence interval (CI] = 1.211–3.262), freshness of foods (OR = 1.767, 95% CI = 1.075–2.886), and variety in foods (OR = 1.961, 95% CI = 1.197–3.212) were significant factors affecting the risk of malnutrition. For frailty, the freshness of foods (OR = 1.997, 95% CI = 1.053–3.788), variety in foods (OR = 2.440, 95% CI = 1.277–4.661), and small purchase of foods (OR = 2.645, 95% CI = 1.362–5.139) were significant environmental factors. In conclusion, we found that the perceived food store environment in the local community can influence the occurrence of malnutrition and frailty in vulnerable, urban older adults.
A Qualitative Study of Motivators, Strategies, Barriers, and Learning Needs Related to Healthy Cooking during Pregnancy
Authors: Garcia Travertine , Duncanson Kerith , Shrewsbury Vanessa A. , Wolfson Julia A.
Published: July 2021

Nutrition during pregnancy has lifelong impacts on the health of mother and child. However, this life stage presents unique challenges to healthy cooking and eating. Cooking interventions show promising results, but often lack theoretical basis and rigorous evaluation. The objective of this formative, qualitative study was to explore motivators, strategies, and barriers related to healthy cooking during pregnancy. Pregnant individuals’ preferences for a cooking education program were also explored. We conducted five focus groups with pregnant individuals (n = 20) in Southeast Michigan in 2019. Focus groups were audio-recorded and transcribed verbatim, then double coded by two members of the research team. Mean gestational age was 18.3 ± 9.6 weeks. Common motivators included feeding other children, avoiding pregnancy complications, promoting fetal growth, and avoiding foodborne illness. Challenges included pregnancy symptoms, navigating nutrition recommendations, mental energy of meal planning, family preferences, and time constraints. Strategies employed were meal planning and including a variety of foods. Participants identified organizational strategies, recipes, nutrition information, and peer support as important components of a cooking intervention during pregnancy. This study characterized multiple challenges to healthy home cooking during pregnancy, providing novel insight to inform the development of cooking skills education programs during this important life stage.
The Development of Feeding and Eating Disorders after Bariatric Surgery: A Systematic Review and Meta-Analysis
Authors: Taba João , Suzuki Milena , Nascimento Fernanda , Iuamoto Leandro , Hsing Wu , Pipek Leonardo , Carneiro-D’Albuquerque Luiz , Meyer Alberto , Andraus Wellington
Published: July 2021

Background: Patients in the postoperative period following bariatric surgery are at risk of developing eating disorders. This study aims to analyze the relation between bariatric surgery and the development and recurrence of eating disorders. Material and methods: A literature review was carried out on 15 November 2020. Fourteen studies that met the eligibility criteria were included for qualitative synthesis, and 7 studies for meta-analysis. Results: The prevalence of eating disorders in the postoperative period was 7.83%, based on the 7 studies in the meta-analysis. Binge eating disorder alone was 3.81%, which was the most significant factor, and addressed in 6 of these studies. Conclusion: The investigated studies have significant methodological limitations in assessing the relation between bariatric surgery and eating disorders, since they mostly present data on prevalence. PROSPERO CRD42019135614.
Vegan Diet and the Gut Microbiota Composition in Healthy Adults
Authors: Losno Emily A. , Sieferle Katharina , Perez-Cueto Federico J. Armando , Ritz Christian
Published: July 2021

The human gut microbiota are the microorganisms (generally bacteria and archaea) that live in the digestive tracts of humans. Due to their numerous functions, the gut microbiota can be considered a virtual organ of the body, playing a pivotal role in health maintenance. Dietary habits contribute to gut microbiota composition, and evidence from observational and intervention studies suggest that vegan diets may promote health, potentially through affecting the diverse ecosystem of beneficial bacteria in the gut. A systematic literature search was conducted on PubMed and Scopus to identify studies investigating the microbiota composition in vegans. Vegans are defined as people excluding food products that are derived from animals from their diet. Nine observational studies were identified. The main outcome of the systematic review was an increase in Bacteroidetes on the phylum level and a higher abundance of Prevotella on the genus level. In conclusion, the present systematic literature review highlighted some benefits of a vegan diet but also demonstrated the complexity of evaluating results from gut microbiota research. The available evidence only consisted of cross-sectional studies, therefore suggesting the need for well-designed randomised controlled trials. Furthermore, the quality assessment of the studies included in the review suggested a lack of standardised and validated methods for participant selection as well as for faecal sampling and faecal analysis.
Evaluation of HPV-Related Biomarkers in Anal Cytological Samples from HIV-Uninfected and HIV-Infected MSM
Authors: Rollo Francesca , Latini Alessandra , Giuliani Massimo , Giglio Amalia , Donà Maria Gabriella , Benevolo Maria
Published: July 2021

Men who have sex with men (MSM) harbor the highest risk for anal carcinoma, mainly caused by Human Papillomavirus (HPV). The use of HPV-related biomarkers in the screening for this neoplasia is still debated. We assessed the association between high-risk (hr)HPV DNA, HPV16/18 DNA, hrHPV E6/E7 mRNA, and p16/Ki-67 with cytological abnormalities (any grade) and high-grade intraepithelial lesions (HSIL) in HIV-uninfected and HIV-infected MSM. Overall, 150 cytological samples in PreservCyt (Hologic), with a negative to HSIL report, were analyzed for hrHPV DNA, hrHPV E6/E7 mRNA, and p16/Ki-67 using the Linear Array (Roche), Aptima (Hologic), and CINtec® PLUS (Roche) assays. In HIV-infected MSM, positivity for all the biomarkers significantly increased with the cytological grade. In both populations, the association of hrHPV E6/E7 mRNA and p16/Ki-67 positivity with HPV16 did not differ significantly compared to hrHPVs other than HPV16. In HIV-uninfected MSM, the odds of having an HSIL increased approximately six times for the p16/Ki-67 positive cases. In HIV-infected individuals, all the biomarkers showed a significant association with HSIL, except for hrHPV DNA, with the strongest association observed for p16/Ki-67. The odds of HSIL increased almost 21 times in those positive for this biomarker. Our results encourage further investigation on the use of p16/Ki-67 dual staining in anal cancer screening for HIV-uninfected and HIV-infected MSM.
Zoonotic Malaria: Non-Laverania Plasmodium Biology and Invasion Mechanisms
Authors: Hang Jing-Wen , Tukijan Farhana , Lee Erica-Qian-Hui , Abdeen Shifana Raja , Aniweh Yaw , Malleret Benoit
Published: July 2021

Malaria, which is caused by Plasmodium parasites through Anopheles mosquito transmission, remains one of the most life-threatening diseases affecting hundreds of millions of people worldwide every year. Plasmodium vivax, which accounts for the majority of cases of recurring malaria caused by the Plasmodium (non-Laverania) subgenus, is an ancient and continuing zoonosis originating from monkey hosts probably outside Africa. The emergence of other zoonotic malarias (P. knowlesi, P. cynomolgi, and P. simium) further highlights the seriousness of the disease. The severity of this epidemic disease is dependent on many factors, including the parasite characteristics, host-parasite interactions, and the pathology of the infection. Successful infection depends on the ability of the parasite to invade the host; however, little is known about the parasite invasion biology and mechanisms. The lack of this information adds to the challenges to malaria control and elimination, hence enhancing the potential for continuation of this zoonosis. Here, we review the literature describing the characteristics, distribution, and genome details of the parasites, as well as host specificity, host-parasite interactions, and parasite pathology. This information will provide the basis of a greater understanding of the epidemiology and pathogenesis of malaria to support future development of strategies for the control and prevention of this zoonotic infection.
Models for Studying the Distribution of Ticks and Tick-Borne Diseases in Animals: A Systematic Review and a Meta-Analysis with a Focus on Africa
Authors: Zannou Olivier M. , Ouedraogo Achille S. , Biguezoton Abel S. , Abatih Emmanuel , Coral-Almeida Marco , Farougou Souaïbou , Yao Kouassi Patrick , Lempereur Laetitia , Saegerman Claude
Published: July 2021

Ticks and tick-borne diseases (TTBD) are constraints to the development of livestock and induce potential human health problems. The worldwide distribution of ticks is not homogenous. Some places are ecologically suitable for ticks but they are not introduced in these areas yet. The absence or low density of hosts is a factor affecting the dissemination of the parasite. To understand the process of introduction and spread of TTBD in different areas, and forecast their presence, scientists developed different models (e.g., predictive models and explicative models). This study aimed to identify models developed by researchers to analyze the TTBD distribution and to assess the performance of these various models with a meta-analysis. A literature search was implemented with PRISMA protocol in two online databases (Scopus and PubMed). The selected articles were classified according to country, type of models and the objective of the modeling. Sensitivity, specificity and accuracy available data of these models were used to evaluate their performance using a meta-analysis. One hundred studies were identified in which seven tick genera were modeled, with Ixodes the most frequently modeled. Additionally, 13 genera of tick-borne pathogens were also modeled, with Borrelia the most frequently modeled. Twenty-three different models were identified and the most frequently used are the generalized linear model representing 26.67% and the maximum entropy model representing 24.17%. A focus on TTBD modeling in Africa showed that, respectively, genus Rhipicephalus and Theileria parva were the most modeled. A meta-analysis on the quality of 20 models revealed that maximum entropy, linear discriminant analysis, and the ecological niche factor analysis models had, respectively, the highest sensitivity, specificity, and area under the curve effect size among all the selected models. Modeling TTBD is highly relevant for predicting their distribution and preventing their adverse effect on animal and human health and the economy. Related results of such analyses are useful to build prevention and/or control programs by veterinary and public health authorities.
G-Quadruplex-Based Drug Delivery Systems for Cancer Therapy
Authors: Lopes-Nunes Jéssica , Oliveira Paula , Cruz Carla
Published: July 2021

G-quadruplexes (G4s) are a class of nucleic acids (DNA and RNA) with single-stranded G-rich sequences. Owing to the selectivity of some G4s, they are emerging as targeting agents to overtake side effects of several potential anticancer drugs, and delivery systems of small molecules to malignant cells, through their high affinity or complementarity to specific targets. Moreover, different systems are being used to improve their potential, such as gold nano-particles or liposomes. Thus, the present review provides relevant data about the different studies with G4s as drug delivery systems and the challenges that must be overcome in the future research.
Effect of Storage Humidity on Physical Stability of Spray-Dried Naproxen Amorphous Solid Dispersions with Polyvinylpyrrolidone: Two Fluid Nozzle vs. Three Fluid Nozzle
Authors: Bhujbal Sonal V. , Su Yongchao , Pathak Vaibhav , Zemlyanov Dmitry Y. , Cavallaro Alex-Anthony , Munson Eric J. , Taylor Lynne S. , Zhou Qi (Tony)
Published: July 2021

In a spray drying operation, a two-fluid nozzle (2FN) with a single channel is commonly used for atomizing the feed solution. However, the less commonly used three-fluid nozzle (3FN) has two separate channels, which allow spray drying of materials in two incompatible solution systems. Although amorphous solid dispersions (ASDs) prepared using a 3FN have been reported to deliver comparable drug dissolution performance relative to those prepared using a 2FN, few studies have systematically examined the effect of 3FN on the physical stability. Therefore, the goal of this work is to systematically study the physical stability of ASDs that are spray-dried using a 3FN compared to those prepared using the traditional 2FN. For the 2FN, a single solution of naproxen and polyvinylpyrrolidone (PVP) was prepared in a mixture of acetone and water at a 1:1 volume ratio because 2FN allows for only one solution inlet. For the 3FN, naproxen and PVP were dissolved individually in acetone and water, respectively, because 3FN allows simultaneous entry of two solutions. Upon storage of the formulated ASDs at different humidity levels (25%, 55% and 75% RH), naproxen crystallized more quickly from the 3FN ASDs as compared with the 2FN ASDs. 3FN ASDs crystallized after 5 days of storage at all conditions, whereas 2FN ASDs did not crystallize even at 55% RH for two months. This relatively higher crystallization tendency of 3FN ASDs was attributed to the inhomogeneity of drug and polymers as identified by the solid-state Nuclear Magnetic Resonance findings, specifically due to poor mixing of water- and acetone-based solutions at the 3FN nozzle. When only acetone was used as a solvent to prepare drug-polymer solutions for 3FN, the formulated ASD was found to be stable for >3 months of storage (at 75% RH), which suggests that instability of the 3FN ASD was due to the insufficient mixing of water and acetone solutions. This study provides insights into the effects of solvent and nozzle choices on the physical stability of spray-dried ASDs.
Evaluation of Nutritional, Phytochemical, and Mineral Composition of Selected Medicinal Plants for Therapeutic Uses from Cold Desert of Western Himalaya
Authors: Radha  , Kumar Manoj , Puri Sunil , Pundir Ashok , Bangar Sneh Punia , Changan Sushil , Choudhary Poonam , Parameswari E. , Alhariri Ahmad , Samota Mahesh Kumar , Damale Rahul D. , Singh Surinder , Berwal Mukesh K. , Dhumal Sangram , Bhoite Anilkumar G. , Senapathy M. , Sharma Anshu , Bhushan Bharat , Mekhemar Mohamed
Published: July 2021

The aim of this study was to determine the elemental and nutritive values of leaf parts of 10 selected wild medicinal plants, Acer pictum, Acer caecium, Betula utilis, Oxalis corniculata, Euphorbia pilosa, Heracleum lanatum, Urtica dioica, Berberis lycium, Berberis asiaticaand, and Quercus ilex, collected from the high hills of the Chitkul range in district Kinnaur, Western Himalaya. The nutritional characteristics of medicinal plant species were analyzed by using muffle furnace and micro-Kjeldahl methods, and the mineral content in plants was analyzed through atomic absorption spectrometry. The highest percentage of used value was reported in Betula utilis (0.42) and the lowest in Quercus ilex (0.17). In this study, it was found that new generations are not much interested in traditional knowledge of ethnomedicinal plants due to modernization in society. Therefore, there is an urgent need to document ethnomedicinal plants along with their phytochemical and minerals analysis in study sites. It was found that rural people in western Himalaya are dependent on wild medicinal plants, and certain steps must be taken to conserve these plants from extinction in the cold desert of Himalayan region. They are an alternative source of medicine because they contain saponin, alkaloid, and flavonoid etc. as well as minerals. The leaves used for analysis possesses good mineral content, such as Na, N, K, P, Zn, Fe, Cu, Mn, Ca, Mg, and S. Hence, in the current study it was observed that medicinal plants are not only used for therapeutic purposes, but they can also be used as nutritional supplements.
Physiological Responses and Gene Expression Patterns in Open-Pollinated Seedlings of a Pummelo-Mandarin Hybrid Rootstock Exposed to Salt Stress and Huanglongbing
Authors: Mahmoud Lamiaa M. , Huyck Patrick J. , Vincent Christopher I. , Gmitter Frederick G. , Grosser Jude W. , Dutt Manjul
Published: July 2021

Huanglongbing (HLB), caused by the phloem-limited bacterium Candidatus Liberibacter asiaticus (CaLas), is the primary biotic stress causing significant economic damage to the global citrus industry. Among the abiotic stresses, salinity affects citrus production worldwide, especially in arid and coastal regions. In this study, we evaluated open-pollinated seedlings of the S10 (a diploid rootstock produced from a cross between two siblings of the Hirado Buntan Pink pummelo (Citrus maxima (Burm.) Merr.) with the Shekwasha mandarin (Citrus reticulata Blanco)) for their ability to tolerate HLB and salinity stresses. In a greenhouse study, ‘Valencia’ sweet orange (either HLB-positive or negative) was grafted onto six clonally propagated lines generated from the screened seedlings in the greenhouse and the trees were irrigated with 150 mM NaCl after eight months of successful grafting and detection of CaLas in the leaf petioles. Cleopatra mandarin was used as a salt-tolerant and HLB-sensitive rootstock control. CaLas infection was monitored using a quantitative polymerase chain reaction before and after NaCl treatments. Following three months of NaCl treatment, ‘Valencia’ leaves on the S10 rootstock seedlings recorded lower levels of chlorophyll content compared to Cleopatra under similar conditions. Malondialdehyde content was higher in HLB-infected ‘Valencia’ grafted onto Cleopatra than in the S10 lines. Several plant defense-related genes were significantly upregulated in the S10 lines. Antioxidant and Na+ co-transporter genes were differentially regulated in these lines. Based on our results, selected S10 lines have potential as salt-tolerant rootstocks of ‘Valencia’ sweet orange under endemic HLB conditions. However, it is necessary to propagate selected lines through tissue culture or cuttings because of the high percentage of zygotic seedlings derived from S10.
Catalytic and Photocatalytic Electrospun Nanofibers for Hydrogen Generation from Ammonia Borane Complex: A Review
Authors: Abutaleb Ahmed
Published: July 2021

Hydrogen (H2) is a promising renewable energy source that can replace fossil fuels since it can solve several environmental and economic issues. However, the widespread usage of H2 is constrained by its storage and safety issues. Many researchers consider solid materials with an excellent capacity for H2 storage and generation as the solution for most H2-related issues. Among solid materials, ammonia borane (abbreviated hereafter as AB) is considered one of the best hydrogen storage materials due to its extraordinary H2 content and small density. However, the process must be conducted in the presence of efficient catalysts to obtain a reasonable amount of generated H2. Electrospun nanofibrous catalysts are a new class of efficient catalysts that involves the usage of polymers. Here, a comprehensive review of the ceramic-supported electrospun NF catalysts for AB hydrolysis is presented, with a special focus on catalytic and photolytic performance and preparation steps. Photocatalytic AB hydrolysis was discussed in detail due to its importance and promising results. AB photocatalytic hydrolysis mechanisms under light were also explained. Electrospun catalysts show excellent activity for AB hydrolysis with good recyclability. Kinetics studies show that the AB hydrolysis reaction is independent of AB concentration and the first-order reaction of NF catalysts.
Characterization of Hybrid Composites with Polyester Waste Fibers, Olive Root Fibers and Coir Pith Micro-Particles Using Mixture Design Analysis for Structural Applications
Authors: Tufail Muhammad Rizwan , Jamshaid Hafsa , Mishra Rajesh , Hussain Uzair , Tichy Martin , Muller Miroslav
Published: July 2021

In the present work, hybrid composites were developed by using polyester waste fibers along with natural origin materials: olive root fibers and coir pitch filler. Such composite panels can be used as a potential alternative for fiber glass sunshade panels and room dividers in buildings. Hybrid composites were fabricated by mixing polyester waste fibers and olive root fibers in different ratios (0:100, 33:67, 67:33 and 100:0). Coir pith micro-particles with an average size of 312 d.nm were used as filler in the polyester matrix at three different levels (0%, 5%, and 10%) of the overall matrix weight. Mechanical properties, e.g., tensile strength, flexural strength and impact strength, thermal properties, e.g., coefficient of linear thermal expansion, thermo-gravimetric analysis (TGA) and environmental properties, e.g., water absorption, loss of density after exposure to weathering were characterized. For comparison purposes, a commercially available fiber glass sunshades sample was also investigated. Mixture design analysis was used to optimize the ratio of all components in the composite. Graphical comparison of experimental results using regression models showed a high degree of correlation. An optimized formulation of composite with an objective of maximization of tensile strength, flexural strength, impact strength and minimization of water absorption, density loss, as well as coefficient of linear thermal expansion, was determined at 70.83 wt%, 15.15 wt%, and 14.01 wt% of polyester waste fibers, olive root fibers and coir pith micro-fillers, respectively. Overall, it can be concluded that the developed hybrid composites from waste fibrous materials can be used as a promising alternative and a value-added application in buildings and construction purposes.
Photoinduced Processes as a Way to Sustainable Polymers and Innovation in Polymeric Materials
Authors: Bongiovanni Roberta , Vacche Sara Dalle , Vitale Alessandra
Published: July 2021

Photoinduced processes have gained considerable attention in polymer science and have greatly implemented the technological developments of new products. Therefore, a large amount of research work is currently developed in this area: in this paper we illustrate the advantages of a chemistry driven by light, the present perspectives of the technology, and summarize some of our recent research works, honoring the memory of Prof. Aldo Priola who passed away in March 2021 and was one of the first scientists in Italy to contribute to the field.
An Overview on Collagen and Gelatin-Based Cryogels: Fabrication, Classification, Properties and Biomedical Applications
Authors: He Yujing , Wang Chunhua , Wang Chenzhi , Xiao Yuanhang , Lin Wei
Published: July 2021

Decades of research into cryogels have resulted in the development of many types of cryogels for various applications. Collagen and gelatin possess nontoxicity, intrinsic gel-forming ability and physicochemical properties, and excellent biocompatibility and biodegradability, making them very desirable candidates for the fabrication of cryogels. Collagen-based cryogels (CBCs) and gelatin-based cryogels (GBCs) have been successfully applied as three-dimensional substrates for cell culture and have shown promise for biomedical use. A key point in the development of CBCs and GBCs is the quantitative and precise characterization of their properties and their correlation with preparation process and parameters, enabling these cryogels to be tuned to match engineering requirements. Great efforts have been devoted to fabricating these types of cryogels and exploring their potential biomedical application. However, to the best of our knowledge, no comprehensive overviews focused on CBCs and GBCs have been reported currently. In this review, we attempt to provide insight into the recent advances on such kinds of cryogels, including their fabrication methods and structural properties, as well as potential biomedical applications.
Methotrexate-Loaded Gelatin and Polyvinyl Alcohol (Gel/PVA) Hydrogel as a pH-Sensitive Matrix
Authors: Akhlaq Muhammad , Azad Abul Kalam , Ullah Inam , Nawaz Asif , Safdar Muhammad , Bhattacharya Tanima , Uddin A. B. M. Helal , Abbas Syed Atif , Mathews Allan , Kundu Sukalyan Kumar , Miret Mireia Mallandrich , Murthy H. C. Ananda , Nagaswarupa H. P.
Published: July 2021

The aim was to formulate and evaluate Gel/PVA hydrogels as a pH-sensitive matrix to deliver methotrexate (MTX) to colon. The primed Gel/PVA hydrogels were subjected to evaluation for swelling behavior, diffusion coefficient, sol-gel characteristic and porosity using an acidic (pH 1.2) and phosphate buffer (PBS) (pH 6.8 & pH 7.4) media. Fourier transform infrared spectroscopy (FTIR) and thermal gravimetric analysis (TGA) were performed to evaluate the chemical compatibility of the Gel/PVA hydrogel. The shape alteration and release of Gel/PVA hydrogel was conducted at pH 1.2, pH 6.8 and pH 7.4. The drug release kinetic mechanism was determined using various kinetic equations. The physicochemical evaluation tests and drug release profile results were found to be significant (p < 0.01). However, it was dependent on the polymers’ concentration, the pH of the release media and the amount of the cross-linking agent. Hydrogels containing the maximum amount of gel showed a dynamic equilibrium of 10.09 ± 0.18 and drug release of 93.75 ± 0.13% at pH 1.2. The kinetic models showed the release of MTX from the Gel/PVA hydrogel was non-Fickian. The results confirmed that the newly formed Gel/PVA hydrogels are potential drug delivery systems for a controlled delivery of MTX to the colon.
Photocuring Hyaluronic Acid/Silk Fibroin Hydrogel Containing Curcumin Loaded CHITOSAN Nanoparticles for the Treatment of MG-63 Cells and ME3T3-E1 Cells
Authors: Yu Qingwen , Meng Zhiyuan , Liu Yichao , Li Zehao , Sun Xing , Zhao Zheng
Published: July 2021

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.
Investigating the Viability of Epithelial Cells on Polymer Based Thin-Films
Authors: Vihar Boštjan , Rožanc Jan , Krajnc Boštjan , Gradišnik Lidija , Milojević Marko , Činč Ćurić Laura , Maver Uroš
Published: July 2021

The development of novel polymer-based materials opens up possibilities for several novel applications, such as advanced wound dressings, bioinks for 3D biofabrication, drug delivery systems, etc. The aim of this study was to evaluate the viability of vascular and intestinal epithelial cells on different polymers as a selection procedure for more advanced cell-polymer applications. In addition, possible correlations between increased cell viability and material properties were investigated. Twelve polymers were selected, and thin films were prepared by dissolution and spin coating on silicon wafers. The prepared thin films were structurally characterized by Fourier transform infrared spectroscopy, atomic force microscopy, and goniometry. Their biocompatibility was determined using two epithelial cell lines (human umbilical vein endothelial cells and human intestinal epithelial cells), assessing the metabolic activity, cell density, and morphology. The tested cell lines showed different preferences regarding the culture substrate. No clear correlation was found between viability and individual substrate characteristics, suggesting that complex synergistic effects may play an important role in substrate design. These results show that a systematic approach is required to compare the biocompatibility of simple cell culture substrates as well as more complex applications (e.g., bioinks).
Shear-Induced and Nanofiber-Nucleated Crystallization of Novel Aliphatic–Aromatic Copolyesters Delineated for In Situ Generation of Biodegradable Nanocomposites
Authors: Hosseinnezhad Ramin
Published: July 2021

The shear-induced and cellulose-nanofiber nucleated crystallization of two novel aliphatic–aromatic copolyesters is outlined due to its significance for the in situ generation of biodegradable nanocomposites, which require the crystallization of nanofibrous sheared inclusions at higher temperatures. The shear-induced non-isothermal crystallization of two copolyesters, namely, poly(butylene adipate-co-succinate-co-glutarate-co-terephthalate) (PBASGT) and poly(butylene adipate-co-terephthalate) (PBAT), was studied following a light depolarization technique. To have a deep insight into the process, the effects of the shear rate, shear time, shearing temperature and cooling rate on the initiation, kinetics, growth and termination of crystals were investigated. Films of 60 μm were subjected to various shear rates (100–800 s−1) for different time intervals during cooling. The effects of the shearing time and increasing the shear rate were found to be an elevated crystallization temperature, increased nucleation density, reduced growth size of lamella stacks and decreased crystallization time. Due to the boosted nucleation sites, the nuclei impinged with each other quickly and growth was hindered. The effect of the cooling rate was more significant at lower shear rates. Shearing the samples at lower temperatures, but still above the nominal melting point, further shifted the non-isothermal crystallization to higher temperatures. As a result of cellulose nanofibers’ presence, the crystallization of PBAT, analyzed by DSC, was shifted to higher temperatures.
Popular-Indigenous Catholicism in Southern Mexico
Authors: Norget Kristin
Published: July 2021

This paper examines popular indigenous religiosity in the southern Mexican state of Oaxaca in the 1990s, in the context of a “progressive” pastoral program formed within the campaign of the New Evangelization, and attuned to the region’s large indigenous population. Based on ethnographic research in an urban Oaxacan context, I offer an account of the popular Catholic ritualization of death which highlights its independence, and sensuous, material, collective orientation. I approach popular Catholicism as a field of potential tension, hybridity, and indeterminacy, encompassing the discourses and teachings of the Catholic Church in continuous interaction with people’s own sacred imaginaries and domestic devotional practices.
Performance Evaluation of Four Ocean Reflectance Model
Authors: Li Jun , Li Tongji , Song Qingjun , Ma Chaofei
Published: July 2021

Phytoplankton are the main factors influencing light under the sea surface in Case Ι water. The ocean reflectance model (ORM), which takes into account the chlorophyll a concentration data, can calculate the remote sensing reflectance of Case Ι water. In this study, we examined the differences and performance of four ORMs, including Morel and Maritorena (2001, MM01), Morel and Gentili (2007, MG07), Mobley (2014, MO14), and Hydrolight Abcase1 Lookup Tables. The differences between the four ORMs in terms of their absorption and backscattering coefficients were evaluated. Preformation of the four ORMs was compared using the NASA bio-Optical Marine Algorithm Dataset and in situ data from the South China Sea. The results showed that preformation of MM01 was the best.
Optical Property Model for Cirrus Clouds Based on Airborne Multi-Angle Polarization Observations
Authors: Wang Yi , Yang Ping , King Michael D. , Baum Bryan A.
Published: July 2021

We present an improved remote sensing technique to infer an optimal habit/shape model for ice particles in cirrus clouds using multi-angle polarimetric measurements at 865 nm made by the Airborne Multi-angle SpectroPolarimeter Imager (AirMSPI) instrument. The common method of ice model inference is based on intensity (total reflectivity) measurements, which is generally not applicable to optically thin ice clouds (i.e., cirrus clouds) where single scattering dominates. The new approach is able to infer an ice model in clouds with optical thicknesses smaller than 5. The improvement is made by first assuming the optical thickness retrieved using total reflectivity. Subsequently, the polarized reflectivity is calculated based on look-up tables generated from simulated polarized reflectances computed for cirrus clouds in conjunction with eight ice particle models. The ice particle model that leads to the closest fit to the measurements is regarded as the optimal ice particle model. Additionally, an alternative method is applied that does not consider polarized reflectivity. These two methods are applied to a data sample as a proof-of-concept study where AirMSPI observed a single cirrus layer. In this case study, the hexagonal column aggregate model works for most pixels both with and without considering polarized reflectivities. The retrieval cost function is high when the camera pairs with large zenith angles are included in the retrievals. This result suggests that further studies will be necessary to have a better understanding of all eight selected ice particle models at scattering angles smaller than 100°.
Development of a Flash Flood Confidence Index from Disaster Reports and Geophysical Susceptibility
Authors: Kruczkiewicz Andrew , Bucherie Agathe , Ayala Fernanda , Hultquist Carolynne , Vergara Humberto , Mason Simon , Bazo Juan , de Sherbinin Alex
Published: July 2021

The analysis of historical disaster events is a critical step towards understanding current risk levels and changes in disaster risk over time. Disaster databases are potentially useful tools for exploring trends, however, criteria for inclusion of events and for associated descriptive characteristics is not standardized. For example, some databases include only primary disaster types, such as ‘flood’, while others include subtypes, such as ‘coastal flood’ and ‘flash flood’. Here we outline a method to identify candidate events for assignment of a specific disaster subtype—namely, ‘flash floods’—from the corresponding primary disaster type—namely, ‘flood’. Geophysical data, including variables derived from remote sensing, are integrated to develop an enhanced flash flood confidence index, consisting of both a flash flood confidence index based on text mining of disaster reports and a flash flood susceptibility index from remote sensing derived geophysical data. This method was applied to a historical flood event dataset covering Ecuador. Results indicate the potential value of disaggregating events labeled as a primary disaster type into events of a particular subtype. The outputs are potentially useful for disaster risk reduction and vulnerability assessment if appropriately evaluated for fitness of use.