bluuebunny/crossref_metadata_2025 huggingface dataset:
Local and Non-local Effects of Foam Rolling on Passive Soft Tissue Properties and Spinal Excitability
Authors: Nakamura Masatoshi , Konrad Andreas , Kiyono Ryosuke , Sato Shigeru , Yahata Kaoru , Yoshida Riku , Yasaka Koki , Murakami Yuta , Sanuki Futaba , Wilke Jan
Published: June 2021

In sports and clinical settings, roller massage (RM) interventions are used to acutely increase range of motion (ROM); however, the underlying mechanisms are unclear. Apart from changes in soft tissue properties (i.e., reduced passive stiffness), neurophysiological alterations such as decreased spinal excitability have been described. However, to date, no study has investigated both jointly. The purpose of this trial was to examine RM’s effects on neurophysiological markers and passive tissue properties of the plantar flexors in the treated (ROLL) and non-treated (NO-ROLL) leg. Fifteen healthy individuals (23 ± 3 years, eight females) performed three unilateral 60-s bouts of calf RM. This procedure was repeated four times on separate days to allow independent assessments of the following outcomes without reciprocal interactions: dorsiflexion ROM, passive torque during passive dorsiflexion, shear elastic modulus of the medial gastrocnemius muscle, and spinal excitability. Following RM, dorsiflexion ROM increased in both ROLL (+19.7%) and NO-ROLL (+13.9%). Similarly, also passive torque at dorsiflexion ROM increased in ROLL (+15.0%) and NO-ROLL (+15.2%). However, there were no significant changes in shear elastic modulus and spinal excitability (p > 0.05). Moreover, significant correlations were observed between the changes in DF ROM and passive torque at DF ROM in both ROLL and NO-ROLL. Changes in ROM after RM appear to be the result of sensory changes (e.g., passive torque at DF ROM), affecting both rolled and non-rolled body regions. Thus, therapists and exercise professionals may consider applying remote treatments if local loading is contraindicated.
A Moderated-Mediation Analysis of Organizational Justice and Leader-Member Exchange: Cross-Validation With Three Sub-samples
Authors: Shkoler Or , Tziner Aharon , Vasiliu Cristinel , Ghinea Claudiu-Nicolae
Published: June 2021

In an increasingly competitive work world, managers—whose links with subordinates, and their perceptions thereof, are critical components in that relationship—need to monitor employees' mindsets to facilitate their productivity. Our paper investigates organizational justice perceptions as an antecedent to two important outcomes: organizational citizenship behaviors and counterproductive work behaviors. The moderating effect of leader-member exchange and the mediating effect of work motivation were incorporated into a parsimonious moderated-mediation model designed to assist managers in achieving the stated objective. The model was tested on 3,293 Romanian workers, randomly divided into sub-samples of 1,098, 1,098, and 1,097 participants. Indicating high data consistency and credibility for the most part, in each sub-group, all the variables associated as predicted, with the notable exception of LMX. Implications, limitations, and suggestions for future research are discussed, with emphasis on the investigation's cultural context.
Loneliness of the Blind and the Visually Impaired
Authors: Rokach Ami , Berman David , Rose Alison
Published: June 2021

Loneliness has been termed a social epidemic, especially when experienced by people with disabilities. In order to better understand how loneliness is experienced in vulnerable populations, the present study compared the qualitative dimensions of loneliness of the blind and visually impaired with the general population not on the frequency or intensity of their loneliness, but on its qualitative aspects. One hundred and eighty-seven participants responded to a questionnaire which measured the qualitative aspects of loneliness on five subscales: Emotional distress, social inadequacy, Growth and discovery, social isolation, and emotional alienation. Results indicated that as expected, the two populations differed significantly in their scores on four of the five subscales (except emotional alienation), but in the opposite direction of what was expected. That may indicate that the visually impaired person’s ability to transcend their blindness, and connect with those around them, and the larger society, in different—and not necessarily less meaningful-manner than the seeing general population. As expected, the visually impaired scored significantly higher than the general population on the Growth and development subscale.
The Effects of Mindfulness-Based Intervention on Shooting Performance and Cognitive Functions in Archers
Authors: Wu Tsung-Yi , Nien Jui-Ti , Kuan Garry , Wu Chih-Han , Chang Yi-Chieh , Chen Hsueh-Chih , Chang Yu-Kai
Published: June 2021

This study investigated the effects of a mindfulness-based intervention (MBI) called mindfulness-based peak performance (MBPP) on athletic performance and cognitive functions in archers, as well as the role of psychological status and the dose-response relationship of MBPP in archery performance. Twenty-three archers completed a simulated archery competition and the Stroop task prior to and after MBPP training, which consisted of eight sessions over four weeks, while the mindfulness and rumination levels of the archers were assessed at three time points, namely, before, at the mid-point of, and after the MBPP program. The results revealed that the MBPP program significantly improved the shooting performance (p = 0.002, d = 0.27), multiple cognitive functions (ps < 0.001, d = 0.51~0.71), and mindfulness levels of the archers on the post-test, compared to the pre-test (p = 0.032, ηp2 = 0.15 for general; p = 0.004, ηp2 = 0.22 for athletic). Additionally, negative ruminations level was decreased from the pre-test to the middle-test and post-test (ps < 0.001, ηp2 = 0.43). These findings provide preliminary evidence to support the view that MBPP could serve as a promising form of training for fine motor sport performance, cognitive functions, and specific psychological status, such that it warrants further study.
Convolutional Neural Network-Based Human Movement Recognition Algorithm in Sports Analysis
Authors: Liu Jiatian
Published: June 2021

In order to analyse the sports psychology of athletes and to identify the psychology of athletes in their movements, a human action recognition (HAR) algorithm has been designed in this study. First, a HAR model is established based on the convolutional neural network (CNN) to classify the current action state by analysing the action information of a task in the collected videos. Secondly, the psychology of basketball players displaying fake actions during the offensive and defensive process is investigated by combining with related sports psychological theories. Then, the psychology of athletes is also analysed through the collected videos, so as to predict the next response action of the athletes. Experimental results show that the combination of grayscale and red-green-blue (RGB) images can reduce the image loss and effectively improve the recognition accuracy of the model. The optimised convolutional three-dimensional network (C3D) HAR model designed in this study has a recognition accuracy of 80% with an image loss of 5.6. Besides, the time complexity is reduced by 33%. Therefore, the proposed optimised C3D can recognise effectively human actions, and the results of this study can provide a reference for the investigation of the image recognition of human action in sports.
Authoritarianism Beyond Disposition: A Literature Review of Research on Contextual Antecedents
Authors: Schnelle Caroline , Baier Dirk , Hadjar Andreas , Boehnke Klaus
Published: June 2021

A core debate in authoritarianism research relates to the stability of authoritarianism, i.e., whether it is a dispositional phenomenon socialized in early childhood or even genetically predisposed, or whether it is impacted by time-sensitive, exterior conditions. Whereas certain individual authoritarian tendencies emerge as a rather stable personality trait, there is also empirical evidence for a dynamic influence of external factors. This review article provides a conceptual multilevel framework for the study of authoritarianism and offers an insight into the state-of-research on socialization and situational influences, with a particular focus on threat. Findings are discussed with regard to key theories of authoritarianism.
Evaluating Factors Influencing Knowledge-Sharing Behavior of Students in Online Problem-Based Learning
Authors: Wang Wei-Tsong , Lin Ying-Lien
Published: June 2021

Adopting online problem-based learning (OPBL) to internship educational programs is an effective teaching method to stimulate self-directed and collaborative learning and knowledge-sharing behavior (KSB) of students. However, the OPBL collaboration experience is different from the traditional lecture-based learning experience for students. Integrating social identity theory and commitment-trust theory develops a formative research model that explains the KSB of students when using social media tools for the OPBL process. This process encourages social interaction and communication of students, in turn, facilitating the integration of collective intelligence or the creation, sharing, and exchange of knowledge. Data collected from 425 nursing students who studied at seven nursing colleges or medical universities in Taiwan were analyzed using the partial least squares (PLSs) technique. The results indicate that social identification is a crucial antecedent of KSB. Relationship quality plays a vital role in shaping the effects of interpersonal trust and relationship commitment (RC) on KSB during internship periods. The findings can contribute to theoretical discussions and enhance the effectiveness of KSB in the literature of internship and non-internship in the higher education field.
Sense of Purpose Promotes Resilience to Cognitive Deficits Attributable to Depressive Symptoms
Authors: Lewis Nathan A. , Hill Patrick L.
Published: June 2021

Objective

Individuals higher in depressive symptoms commonly present with neuropsychological deficits including poorer memory performance. Sense of purpose in life has been shown to promote resilience to cognitive impairment in older adulthood, but it is unclear whether it may also protect against cognitive deficits associated with higher depressive symptoms.

Method

Cognitive functioning among 4599 older American adults (Mage = 74.33 years, range = 65–104 years, 56.84% female) was examined across a 12-year follow-up period. Depressive symptomatology was assessed at each wave using the 8-item Center for Epidemiologic Studies Depression Scale. Multilevel models assessed the influence of depressive symptoms and the interaction with sense of purpose in life on changes in memory performance and mental status.

Results

Higher depressive symptoms were associated with poorer memory performance at baseline, but did not predict rate of change over time. A negative interaction was observed between sense of purpose in life and depressive symptoms such that individuals higher in purpose experienced a less negative association between depressive symptoms and baseline memory performance. No significant interaction of sense of purpose and depressive symptoms was observed on mental status.

Conclusion

Having a sense of purpose in life may help protect older adults from memory deficits associated with higher depressive symptoms. The present findings underscore the potential for sense of purpose to promote cognitive reserve in older adulthood, allowing individuals to maintain cognitive performance in the face of accruing neurological insults.
Epidemiology of SARS-CoV-2 Infection Evaluated by Immunochromatographic Rapid Testing for the Determination of IgM and IgG Against SARS-CoV-2 in a Cohort of Mask Wearing Workers in the Metal-Mechanical Sector in an Area With a High Incidence of COVID-19
Authors: Esposito Susanna , Neglia Cosimo , Affanni Paola , Colucci Maria Eugenia , Argentiero Alberto , Veronesi Licia , Messina Giulia , Deolmi Michela , Principi Nicola ,   the Crown Study Group
Published: June 2021

Background: Although the diagnosis of new coronavirus 2019 (COVID-19) is made through the identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in respiratory secretions by means of molecular methods, a more accurate estimation of SARS-CoV-2 circulation can be obtained by seroprevalence studies. The main aim of this study was to evaluate the true epidemiology of SARS-CoV-2 infection among workers in the metal-mechanical sector who never stopped working during the pandemic period in an area with a high incidence of COVID-19 and to define whether and how they could continue the work without appreciable risks during a second wave.

Methods: A total of 815 metal-mechanical workers who had never stopped working even during the pandemic period in three different factories in the Emilia-Romagna Region, Italy, and who had always used face masks during working hours, underwent a capillary blood rapid test for the determination of IgM and IgG against SARS-CoV-2 (COVID-19 IgG/IgM Rapid test, PrimaLab, Modena, Italy). In the event of a positive test, a nasopharyngeal was performed and tested for the presence of SARS-CoV-2.

Results: The detection of serum IgG/IgM against SARS-CoV-2 was significantly more common among workers employed in Parma (21/345, 6.1%) than among those employed in Calerno (7/242, 2.9%) or in Spilamberto (3/228, 1.3%) (p <0.001). The analysis of the role of the different variables as predictors of seropositivity for IgG/IgM against SARS-CoV-2 revealed that the presence of specific antibodies was strictly associated with a previous history of COVID-19-like symptoms (odds ratio [OR] 3.95, 95% confidence interval [CI] 1.9–8.2) and household members with COVID-19-like symptoms (OR 2.20, 95% CI 1.04–4.82).

Conclusion: This study shows that seropositivity to SARS-CoV-2 is low even among employees who did not interrupt their work during the lockdown phase in a region with a high incidence of COVID-19. The use of face masks appears effective in the avoidance of the transmission of SARS-CoV-2 in factories even in the presence of asymptomatic or mildly symptomatic workers, suggesting that work activities can continue if adequate infection control measures are used during a second wave.
Developing an Evidence-Based Tool for Planning and Evaluating Vaccination Strategies Aimed at Improving Coverage in Elderly and At-Risk Adult Population
Authors: Calabrò Giovanna Elisa , Carini Elettra , Tognetto Alessia , Mancinelli Silvia , Sarnari Laura , Colamesta Vittoria , Ricciardi Walter , de Waure Chiara ,   The BRAVE Project Expert panel
Published: June 2021

Background: Vaccination coverages need to be constantly maintained and improved with the implementation of vaccination strategies. This paper describes the development of an evidence-based tool to guide their planning and evaluation.

Methods: A scoping review was performed in MEDLINE and institutional websites to search for similar available tools. A first version of the tool was developed considering review results and a four-step method used for the control and continuous improvement of processes and products, namely the Deming cycle. A panel of eight experts was then involved in a Delphi study for the finalization of the tool that was eventually discussed in a face-to-face meeting.

Results: The scoping review found only one document and the first version of the tool was composed of 30 items. After the Delphi first round, 11 additional items were suggested and 5 original items amended. After the Delphi second round 41 items were eventually included. During the face-to-face meeting, 7 items were recognized as requisites for setting vaccination strategies, whereas 17 as relevant ones.

Conclusions: Current public health challenges impose the need for evidence-based tools to organize effective vaccination strategies. Our tool is a first proposal which aims to reflect this focus.
Pedestrian Motor Vehicle Accidents and Fatalities in Botswana-An Epidemiological Study
Authors: Mphela Thuso , Mokoka Thamani , Dithole Kefalotse
Published: June 2021

According to WHO, more than 300,000 pedestrian deaths were recorded globally, accounting for 22% of total road user fatalities in 2019. In 2017, Botswana pedestrian fatalities were considerably high at 32% of the total road accident fatalities recorded. This paper investigated causes, distribution, risk, and offers potential solutions to pedestrian accidents and fatalities in Botswana. Secondary data extracted from different sources were analyzed through descriptive statistics and stepwise regression modeling was performed to determine significant explanators of pedestrian fatalities. The results show that the “car” vehicle type is responsible for 55% of pedestrian collisions. There is a higher chance of pedestrian fatality when collision is with a lorry with a trailer. On the other hand, driver negligence is also blamed for 55% of pedestrian accidents. Ninety percent (90%) of pedestrian fatalities happen away from road junctions. Truck, fuel imports and nighttime accidents were identified as significant regressors for pedestrian fatalities. Overall, pedestrians are six times more likely to perish in a collision than their counterparts. Adopting sustainable road infrastructure patterns that promote pedestrian safety will foster mode split. Improving road lighting and infusing pedestrian safety into driver training curriculum, while strengthening road traffic law enforcement, will lead to improved pedestrian safety. Future studies should disaggregate pedestrian safety analysis to account for location-specific variations.
Deletion of the Transcriptional Regulator MucR in Brucella canis Affects Stress Responses and Bacterial Virulence
Authors: Sun Jiali , Dong Hao , Peng Xiaowei , Liu Yufu , Jiang Hui , Feng Yu , Li Qiaoling , Zhu Liangquan , Qin Yuming , Ding Jiabo
Published: June 2021

The transcriptional regulator MucR is related to normal growth, stress responses and Brucella virulence, and affects the expression of various virulence-related genes in smooth-type Brucella strains. However, the function of MucR in the rough-type Brucella canis remains unknown. In this study, we discovered that MucR protein was involved in resistance to heat stress, iron-limitation, and various antibiotics in B. canis. In addition, the expression level of various bacterial flagellum-related genes was altered in mucR mutant strain. Deletion of this transcriptional regulator in B. canis significantly affected Brucella virulence in RAW264.7 macrophage and mice infection model. To gain insight into the genetic basis for distinctive phenotypic properties exhibited by mucR mutant strain, RNA-seq was performed and the result showed that various genes involved in translation, ribosomal structure and biogenesis, signal transduction mechanisms, energy production, and conversion were significantly differently expressed in ΔmucR strain. Overall, these studies have not only discovered the phenotype of mucR mutant strain but also preliminarily uncovered the molecular mechanism between the transcriptional regulator MucR, stress response and bacterial virulence in B. canis.
Message Delivery Strategy Influences Willingness to Comply With Biosecurity
Authors: Merrill Scott C. , Trinity Luke , Clark Eric M. , Shrum Trisha R. , Koliba Christopher J. , Zia Asim , Bucini Gabriela , Sellnow Timothy L. , Sellnow Deanna D. , Smith Julia M.
Published: June 2021

As the Covid-19 pandemic continues worldwide, it has become increasingly clear that effective communication of disease transmission risks associated with protective behaviors is essential, and that communication tactics are not ubiquitously and homogenously understood. Analogous to Covid-19, communicable diseases in the hog industry result in millions of animal deaths and in the United States costs hundreds of millions of dollars annually. Protective behaviors such as preventative biosecurity practices are implemented to reduce these costs. Yet even with the knowledge of the importance of biosecurity, these practices are not employed consistently. The efficacy of biosecurity practices relies on consistent implementation and is influenced by a variety of behavioral factors under the umbrella of human decision-making. Using an experimental game, we collected data to quantify how different messages that described the likelihood of a disease incursion would influence willingness to follow biosecurity practices. Here we show that graphical messages combined with linguistic phrases demarking infection risk levels are more effective for ensuring compliance with biosecurity practices, as contrasted with either simple linguistic phrases or graphical messages with numeric demarcation of risk levels. All three of these delivery methods appear to be more effective than using a simple numeric value to describe probability of infection. Situationally, we saw greater than a 3-fold increase in compliance by shifting message strategy without changing the infection risk, highlighting the importance of situational awareness and context when designing messages.
Parameter Identification of BLDC Motor Using Electromechanical Tests and Recursive Least-Squares Algorithm: Experimental Validation
Authors: Jimenez-Gonzalez Jose , Gonzalez-Montañez Felipe , Jimenez-Mondragon Victor Manuel , Liceaga-Castro Jesús Ulises , Escarela-Perez Rafael , Olivares-Galvan Juan Carlos
Published: June 2021

In this article, the parameter identification of a brushless DC motor (BLDC) is presented. The approach here presented is based on a direct identification considering a three-phase line-to-line voltage electromagnetic torque as function of the electric currents and rotor speed. The estimation is divided into two stages. First, the electrical parameters are estimated by well-known no-load and DC tests. Consequently, estimation of mechanical parameters is performed using a recursive Least Square Algorithm. The proposed approach is validated by comparing model responses to motor real time responses. Additionally, the design, digital simulation and real time implementation of a PI rotor speed controller, based on the estimated model, validate the identification proposal presented here.
Screening of High 1,2-Propanediol Production by Lactobacillus buchneri Strains and Their Effects on Fermentation Characteristics and Aerobic Stability of Whole-Plant Corn Silage
Authors: Huang Zhipeng , Wang Musen , Ke Wencan , Guo Xusheng
Published: June 2021

The study was conducted to screen high 1,2-propanediol produced by Lactobacillus buchneri strains, isolated from baled silages stored for 1 or 2 years, and to evaluate their effects on fermentation quality and aerobic stability of whole-plant corn silage. In total, 31 L. buchneri strains were isolated from alfalfa, whole-plant corn and oat silages. Based on growth performance and 1,2-propanediol and acetic acid production, two strains, L. buchneri 9-2 and L. buchneri 10-1, from alfalfa silage, were further assessed in an ensiling trial on whole-plant corn. The corn silage inoculated with L. buchneri 9-2 or L. buchneri 10-1 had a higher concentration of 1,2-propanediol (34.7 or 34.6 g/kg dry matter (DM)) and acetic acid (47.2 or 45.9 g/kg DM) in comparison with L. buchneri 40788 (reference strain) treated silage (19.5 and 35.9 g/kg DM) after 90 d of fermentation. In addition, these two strains performed better in improving silage aerobic stability relative to control and L. buchneri 40788. The results above indicated that L. buchneri 9-2 and L. buchneri 10-1 could be candidate strains to increase 1,2-propanediol and acetic acid concentrations and improve the aerobic stability of whole-plant corn silage.
Development of Novel Blackgram (Vigna mungo (L.) Hepper) Mutants and Deciphering Genotype × Environment Interaction for Yield-Related Traits of Mutants
Authors: Dhasarathan Manickam , Geetha Seshadri , Karthikeyan Adhimoolam , Sassikumar Datchinamoorthy , Meenakshiganesan Narayanapillai
Published: June 2021

Blackgram (Vigna mungo (L.) Hepper) yields are noticeably poor due to a shortage of improved varieties and an aggravated narrow genetic base. An attempt was made to isolate novel blackgram mutants by selecting for yield-related traits derived through gamma irradiation and testing the mutant genotype’s stability across the different environments. The irradiated blackgram populations M1-M5 were established in the background of cultivars ADT 3, Co 6, and TU 17-9. Desirable mutants were selected from M3 to M5 generations. It was observed in M2 and M3 that gamma rays showed higher mutagenic efficacy and generated good inherited variance for the yield-related traits. M4 established three divergent groups in each blackgram cultivar revealed by clustering analysis. The number of pods per plant, number of clusters per plant, and number of pods per cluster showed a strong direct association with single plant yield and could be considered as selection traits. G × E interactions were higher than the variation due to genotype for single plant yield. Limited environmental interaction was observed for the genotypes G24, G16, G36, G30, and G17, as revealed by AMMI, and the genotypes G18 and G29, as revealed by GGE. GGE biplot revealed the environment-specific genotypes G13 for E1 (Aduthurai), G7 for E2 (Kattuthottam), and G34 for E3 (Vamban) and also portrayed the highly discriminating (E3) and representative (E2) environments. Selected novel blackgram genotypes from this research are useful genetic stocks for genetic improvement and breeding.
Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. I. Rays and Skates
Authors: García-Salinas Pablo , Gallego Victor , Asturiano Juan F.
Published: June 2021

The superorder Batoidea (rays, skates, and relatives), constitutes one of the most threatened group of vertebrates. Strengthening ex situ conservation programs developed in research centers and public aquaria could be a way of addressing this situation. However, captive breeding programs must be improved to prevent the capture of wild animals and to develop proper in situ reintroduction strategies. Sperm extraction and artificial insemination are two techniques commonly used in other threatened species, which could also be used in rays and the like. However, the different reproductive morphologies present within this group of animals may hamper both processes. Here, we present a comparison of the reproductive anatomies of 11 distinct batoid species, emphasizing the important differences between the species when performing sperm extraction or artificial insemination. Both male and female animals, belonging to the Rajidae, Dasyatidae, Torpedinidae and Myliobatidae families, from the Mediterranean Sea were studied. In addition, we describe the procedure to extract sperm using both cannulation and abdominal massage, either from live or dead batoids Finally, the obtention of motile sperm recovered from the oviducal gland of females is described. These techniques generate a new range of possibilities for the conservation of these threatened species.
A Basic Model to Predict Enteric Methane Emission from Dairy Cows and Its Application to Update Operational Models for the National Inventory in Norway
Authors: Niu Puchun , Schwarm Angela , Bonesmo Helge , Kidane Alemayehu , Aspeholen Åby Bente , Storlien Tonje Marie , Kreuzer Michael , Alvarez Clementina , Sommerseth Jon Kristian , Prestløkken Egil
Published: June 2021

The aim of this study was to develop a basic model to predict enteric methane emission from dairy cows and to update operational calculations for the national inventory in Norway. Development of basic models utilized information that is available only from feeding experiments. Basic models were developed using a database with 63 treatment means from 19 studies and were evaluated against an external database (n = 36, from 10 studies) along with other extant models. In total, the basic model database included 99 treatment means from 29 studies with records for enteric CH4 production (MJ/day), dry matter intake (DMI) and dietary nutrient composition. When evaluated by low root mean square prediction errors and high concordance correlation coefficients, the developed basic models that included DMI, dietary concentrations of fatty acids and neutral detergent fiber performed slightly better in predicting CH4 emissions than extant models. In order to propose country-specific values for the CH4 conversion factor Ym (% of gross energy intake partitioned into CH4) and thus to be able to carry out the national inventory for Norway, the existing operational model was updated for the prediction of Ym over a wide range of feeding situations. A simulated operational database containing CH4 production (predicted by the basic model), feed intake and composition, Ym and gross energy intake (GEI), in addition to the predictor variables energy corrected milk yield and dietary concentrate share were used to develop an operational model. Input values of Ym were updated based on the results from the basic models. The predicted Ym ranged from 6.22 to 6.72%. In conclusion, the prediction accuracy of CH4 production from dairy cows was improved with the help of newly published data, which enabled an update of the operational model for calculating the national inventory of CH4 in Norway.
Animal Welfare Attitudes: Effects of Gender and Diet in University Samples from 22 Countries
Authors: Randler Christoph , Adan Ana , Antofie Maria-Mihaela , Arrona-Palacios Arturo , Candido Manecas , Boeve-de Pauw Jelle , Chandrakar Priti , Demirhan Eda , Detsis Vassilis , Di Milia Lee , Fančovičová Jana , Gericke Niklas , Haldar Prasun , Heidari Zeinab , Jankowski Konrad S. , Lehto Juhani E. , Lundell-Creagh Ryan , Medina-Jerez William , Meule Adrian , L. Milfont Taciano , Orgilés Mireia , Morales Alexandra , Natale Vincenzo , Ortiz-Jiménez Xóchitl , Pande Babita , Partonen Timo , Pati Atanu Kumar , Prokop Pavol , Rahafar Arash , Scheuch Martin , Sahu Subhashis , Tomažič Iztok , Tonetti Lorenzo , Vallejo Medina Pablo , van Petegem Peter , Vargas Alejandro , Vollmer Christian
Published: June 2021

Animal Welfare Attitudes (AWA) are defined as human attitudes towards the welfare of animals in different dimensions and settings. Demographic factors, such as age and gender are associated with AWA. The aim of this study was to assess gender differences among university students in a large convenience sample from twenty-two nations in AWA. A total of 7914 people participated in the study (5155 women, 2711 men, 48 diverse). Participants completed a questionnaire that collected demographic data, typical diet and responses to the Composite Respect for Animals Scale Short version (CRAS-S). In addition, we used a measure of gender empowerment from the Human Development Report. The largest variance in AWA was explained by diet, followed by country and gender. In terms of diet, 6385 participants reported to be omnivores, 296 as pescatarian, 637 ate a vegetarian diet and 434 were vegans (n = 162 without answer). Diet was related with CRAS-S scores; people with a vegan diet scored higher in AWA than omnivores. Women scored significantly higher on AWA than men. Furthermore, gender differences in AWA increased as gender inequality decreased.
Evaluation of Nitrogen-Corrected Apparent Metabolizable Energy and Standardized Ileal Amino Acid Digestibility of Different Sources of Rice and Rice Milling Byproducts in Broilers
Authors: Xie Kun , He Xi , Hou De-Xing , Zhang Bingkun , Song Zehe
Published: June 2021

Rice, broken rice (BR), and full-fat rice bran (FFRB) from six different origins were analyzed for their chemical composition, nitrogen-corrected apparent metabolized energy (AMEn), and standardized amino acid digestibility (SIAAD) in 14-day-old and 28-day-old Arbor Acres broilers. Results showed broilers fed with rice and BR had a similar AMEn regardless of the rice and BR having different CP, EE, NDF, ADF, and ash content. FFRB containing significantly different CP, EE, NDF, ADFm and starch presented variable AMEn (p < 0.05), suggesting that starch content in rice and its byproducts contributed most to the AMEn of broilers. The regression equation of AMEn = 14.312 − (0.198 × NDF) and AMEn = 6.491 + (0.103 × Starch) were feasible to integrally predict AMEn of broilers fed to rice and its byproducts. Moreover, 28-day-old broilers had higher SIAAD than 14-day-old ones. The SIAAD of rice were higher than BR and FFRB except for Met, Cys, Thr, and Tyr in 14-day-old broilers (p < 0.05), and the SIAAD of His, Asp, and Ser in BR were higher than FFRB (p < 0.05). In 28-day-old broilers, the SIAAD of Leu, Trp, Asp, Gly, and Pro of rice were still higher than BR and FFRB (p < 0.05), but BR and FFRB had no significant differences (p > 0.05). The regression equations to estimate the SIAAD of Thr, Lys, and Met were: Met = 81.46 + (0.578 × CP), Thr = 0.863 + (6.311 × CP), and Trp = 102.883 − (1.77 × CP), indicating that CP content in rice and its byproducts was likely a major factor for prediction of SIAAD.
Livestock Depredation by Leopards and Tigers Near Bardia National Park, Nepal
Authors: Sijapati Raj Kumar , Sharma Hari Prasad , Sharma Sandhya , Subedi Janak Raj , Belant Jerrold L.
Published: June 2021

Wildlife attacks on livestock near human settlements are increasing due to the proximity of humans to protected areas. These attacks are often severe due to depredations of livestock adversely affecting the livelihoods of people. The nature of carnivore depredations on livestock can differ based on the carnivore species, animal husbandry practices, season, and deterrent technique. We surveyed people living near Bardia National Park (BNP), Nepal, to compare hoofed livestock depredations by leopards (Panthera pardus) and tigers (P. tigris) near (<1 km) and far (>1 km) from this protected area. Overall, 1476 hoofed livestock were reportedly depredated by leopards, and 209 by tigers, during 2015–2019. The number of hoofed livestock killed by leopards each season was, at least, 86% higher than the number killed by tigers. More livestock were killed at BNP irrespective of carnivore deterrent techniques used. Due to severe effects created by livestock depredations near BNP, we recommend using more efficacious deterrent techniques when practical, in addition to improved livestock husbandry practices such as night penning.
Impact of Type of Parturition on Colostrum Microbiota Composition and Puppy Survival
Authors: Kajdič Leonida , Plavec Tanja , Zdovc Irena , Kalin Anja , Zakošek Pipan Maja
Published: June 2021

The objective of our study was to determine whether the type of parturition affects the microbiota of the colostrum and the growth and survival of the puppies. Seventy-nine newborn puppies were divided into three groups regarding the type of parturition: vaginal delivery (VD), elective caesarean section (EL-CS), and emergency caesarean section (EM-CS). After the birth of the puppies, swabs of meconium were collected from the puppies and colostrum was obtained from the dam. Many aerobic and anaerobic bacteria were isolated and identified by mass spectrometry (MALDI-TOF MS). The colostrum microbiota of VD and EL-CS puppies contained a significantly higher abundance of bacteria belonging to the genera Staphylococcus, Kocuria and Enterococcus compared with EM-CS colostrum samples. The composition of the meconium microbiota of the puppies present at birth was similar to the colostrum microbiota of their mothers. It was also found that puppies without a meconium microbiota at birth gained weight more slowly compared with puppies with a meconium microbiota at birth. The type of parturition influenced the bacterial composition of the microbiota in the colostrum. Future studies are necessary to further define the significance of the observed differences in microbiota composition between EM-CS compared with EL-CS and VD colostrum microbiota.
Effect of Body Condition Score, Treatment Period and Month of the Previous Lambing on the Reproductive Resumption of Melatonin-Treated Sarda Breed Sheep during Spring
Authors: Pulinas Luisa , Cosso Giovanni , Mura Maria Consuelo , Carvajal-Serna Melissa , Ouled Ahmed Hatem , Carcangiu Vincenzo , Luridiana Sebastiano
Published: June 2021

Stakeholders place great emphasis upon rationalizing the management and rearing techniques which are utilized within sheep farms. The present study aimed to investigate factors which may improve the reproductive performance of melatonin-treated Sardinian sheep via a series of three trials. The first trial (n = 100) investigated the effect of melatonin treatment alongside body condition score (BCS), the second trial (n = 150) investigated the effect of treatment alongside the date of treatment (treatment period) and the third trial (n = 150) investigated the effect of treatment alongside the previous lambing of the ewes. The findings indicated that melatonin is an effective tool for anticipating and improving the reproductive activity of in Sarda breed sheep during the springtime. Furthermore, to obtain optional results, melatonin implantation should be conducted in April, in ewes that have a BCS of >2.5 and that have passed their third month of lactation.
Effect of Immunocastration and Diet on Growth Performance, Serum Metabolites and Sex Hormones, Reproductive Organ Development and Carcass Quality of Heavy Gilts
Authors: Pérez-Ciria Leticia , Miana-Mena Francisco Javier , Falceto María Victoria , Mitjana Olga , Latorre Maria Angeles
Published: June 2021

It is desirable to increase fatness in gilts destined for Teruel dry-cured ham production. A total of 192 Duroc × (Landrace × Large White) gilts of 40.3 ± 4.80 kg body weight (BW) were used to assess the impact of immunocastration and feeding on growth performance, serum metabolites and sex hormones, reproductive organ development, and carcass quality. Six treatments were arranged factorially (2 × 3) with two types of gilt (entire gilts (EG) vs. immunocastrated gilts (IG)) and three experimental diets (control vs. high energy vs. low crude protein and amino acids) provided from 76 to 134 kg BW (n = 4 per treatment, being the replicate the pen with eight pigs). Immunocastration was carried out at 58 and 77 kg BW. The IG grew faster and showed lighter reproductive tracts and greater fatness than EG. The experimental feeds had limited effect on carcass quality, but the high-energy diet improved gain-to-feed ratio and the low-protein and -amino-acids diet did not impair growth performance. In conclusion, immunocastration was a better strategy than the tested diets to increase the fatness of gilts intended for Teruel dry-cured ham, although increasing energy or decreasing crude protein and amino acid levels in the diet could be beneficial strategies for pig farmers.
Ever-Adapting RND Efflux Pumps in Gram-Negative Multidrug-Resistant Pathogens: A Race against Time
Authors: Zwama Martijn , Nishino Kunihiko
Published: June 2021

The rise in multidrug resistance (MDR) is one of the greatest threats to human health worldwide. MDR in bacterial pathogens is a major challenge in healthcare, as bacterial infections are becoming untreatable by commercially available antibiotics. One of the main causes of MDR is the over-expression of intrinsic and acquired multidrug efflux pumps, belonging to the resistance-nodulation-division (RND) superfamily, which can efflux a wide range of structurally different antibiotics. Besides over-expression, however, recent amino acid substitutions within the pumps themselves—causing an increased drug efflux efficiency—are causing additional worry. In this review, we take a closer look at clinically, environmentally and laboratory-evolved Gram-negative bacterial strains and their decreased drug sensitivity as a result of mutations directly in the RND-type pumps themselves (from Escherichia coli, Salmonella enterica, Neisseria gonorrhoeae, Pseudomonas aeruginosa, Acinetobacter baumannii and Legionella pneumophila). We also focus on the evolution of the efflux pumps by comparing hundreds of efflux pumps to determine where conservation is concentrated and where differences in amino acids can shed light on the broad and even broadening drug recognition. Knowledge of conservation, as well as of novel gain-of-function efflux pump mutations, is essential for the development of novel antibiotics and efflux pump inhibitors.
Molecular Epidemiology of Fosfomycin Resistant E. coli from a Pigeon Farm in China
Authors: Han Lu , Lu Xiao-Qing , Liu Xu-Wei , Liao Mei-Na , Sun Ruan-Yang , Xie Yao , Liao Xiao-Ping , Liu Ya-Hong , Sun Jian , Zhang Rong-Min
Published: June 2021

We determined the prevalence and molecular characteristics of fosfomycin-resistant Escherichia coli from a domestic pigeon farm. A total of 79 samples collected from pigeons and their surrounding environments were screened for the presence of fosfomycin resistant isolates and these included 49 E. coli isolates that displayed high-level resistance (MIC ≥ 256 mg L−1) and carried the fosA3 gene on plasmids with sizes ranging from 80 to 370 kb. MLST analysis of these fosA3-positive E. coli isolates indicated the presence of nine sequence types (ST6856, ST8804, ST457, ST746, ST533, ST165, ST2614, ST362 and ST8805) of which ST6856 was the most prevalent (24.5%, 12/49). PFGE combined with genomic context comparative analyses indicated that the fosA3 gene was spread by horizontal transfer as well as via clonal transmission between E. coli in the pigeon farm, and IS26 played an important role in fosA3 transmission. The high prevalence of fosA3 in the pigeon farm and the high similarity of the fosA3 genomic environment between E. coli isolates from humans and pigeons indicated that the pigeon farm served as a potential reservoir for human infections. The pigeon farm was found to be an important reservoir for the fosA3 gene and this should be further monitored.
Increase of Antimicrobial Consumption in a Tertiary Care Hospital during the First Phase of the COVID-19 Pandemic
Authors: Castro-Lopes Alexandre , Correia Sofia , Leal Cátia , Resende Inês , Soares Pedro , Azevedo Ana , Paiva José-Artur
Published: June 2021

Background: The COVID-19 pandemic poses novel challenges in antimicrobial consumption metrics and stewardship strategies. COVID-19 patients became the major cause of hospital admission during the first wave of the pandemic, often leading to an antimicrobial prescription upon admission or treatment for superinfections. The aim of this study was to understand how antimicrobial consumption was impacted at the beginning of the pandemic in a tertiary care hospital, a reference center for COVID-19. Materials and Methods: A retrospective before-and-after study was done. Descriptive statistics of discharges, patient-days, and antimicrobial use indicators (defined daily doses (DDD)/100 discharges, DDD/100 patient-days) for various groups were calculated for the first three months of the pandemic (March, April, and May 2020) as a quarterly value, and for each year in 2011–2019, and their annual percentage changes were used to estimate 95% confidence intervals. The indicators were compared to patient type (medical/surgical), type of admission (urgent/elective), and age groups using Spearman’s correlation coefficient. Results: Statistically significant increases occurred in 2020 for total antibacterials, macrolides, cephalosporins, amoxicillin/clavulanic acid, carbapenems, meropenem, and third-generation cephalosporins, while a reduction was seen in cefazolin/cefoxitin. A correlation was found between antibacterial consumption and patient or admission type. In 2020, unlike in pre-pandemic years, there was a different impact in DDD/100 discharges and DDD/100 patient-days due to increased lengths-of-stay and longer antimicrobial therapy. Conclusions: The COVID-19 pandemic led to an increase in antimicrobial consumption with a different impact in DDD/100 discharges and DDD/100 patient-days. This highlights the need to use both indicators simultaneously to better understand the causes of antimicrobial consumption variation and improve the design of effective antimicrobial stewardship interventions.
RT001 in Progressive Supranuclear Palsy—Clinical and In-Vitro Observations
Authors: Angelova Plamena R. , Andruska Kristin M. , Midei Mark G. , Barilani Mario , Atwal Paldeep , Tucher Oliver , Milner Peter , Heerinckx Frederic , Shchepinov Mikhail S.
Published: June 2021

Progressive supranuclear palsy (PSP) is a progressive movement disorder associated with lipid peroxidation and intracerebral accumulation of tau. RT001 is a deuterium reinforced isotopologue of linoleic acid that prevents lipid peroxidation (LPO) through the kinetic isotope effect. The effects of RT001 pre-treatment on various oxidative and bioenergetic parameters were evaluated in mesenchymal stem cells (MSC) derived from patients with PSP compared to controls. In parallel, 3 patients with PSP were treated with RT001 and followed clinically. MSCs derived from PSP patients had a significantly higher rate of LPO (161.8 ± 8.2% of control; p < 0.001). A 72-h incubation with RT001 restored the PSP MSCs to normal levels. Mitochondrial reactive oxygen species (ROS) overproduction in PSP-MSCs significantly decreased the level of GSH compared to control MSCs (to 56% and 47% of control; p < 0.05). Incubation with RT001 significantly increased level of GSH in PSP MSCs. The level of mitochondrial DNA in the cells was significantly lower in PSP-MSCs (67.5%), compared to control MSCs. Changes in mitochondrial membrane potential, size, and shape were also observed. Three subjects with possible or probable PSP were treated with RT001 for a mean duration of 26 months. The slope of the PSPRS changed from the historical decline of 0.91 points/month to a mean decline of 0.16 points/month (+/−0.23 SEM). The UPDRS slope changed from an expected increase of 0.95 points/month to an average increase in score of 0.28 points/month (+/−0.41 SEM). MSCs derived from patients with PSP have elevated basal levels of LPO, ROS, and mitochondrial dysfunction. These findings are reversed after incubation with RT001. In PSP patients, the progression of disease may be reduced by treatment with RT001.