bluuebunny/crossref_metadata_2025 huggingface dataset:
Niveles de estigma hacia la tuberculosis en población que acude a los centros de salud de la Red San Juan de Miraflores asociado al diagnóstico de tuberculosis, en los años 2017 y 2019, Lima, Perú
Authors:   ,   Universidad Peruana de Ciencias Aplicadas (UPC), Alania Soria Boris Pavel , Valle Vergara Paul Cristhian
Published: November 2020

Introducción. La tuberculosis es una enfermedad muy prevalente a nivel mundial. Si bien existen numerosas guías sobre cómo tratar y curar esta enfermedad, no existen estudios que evalúen como los determinantes sociales influyen en esta infección. Los determinantes sociales son todas las condiciones sociodemográficas que influyen en nuestro desarrollo, un determinante muy poco estudiando es el estigma hacia la enfermedad de la tuberculosis. Objetivo. Determinar los niveles de estigma hacia la tuberculosis asociados al diagnóstico auto reportado de tuberculosis en pacientes que acuden en los centros de salud de la Red de San Juan de Miraflores, Lima, en los años 2017 y 2019. Material y Métodos. Este es un estudio cuantitativo observacional analítico tipo transversal, realizado en la población que acude a los centros de salud de primer nivel de la red “San Juan de Miraflores”. Se evaluaron 132 participantes, 36 con diagnóstico auto reportado de tuberculosis y 96 sin diagnóstico auto reportado de tuberculosis Resultados. Se encontró que la diferencia de medias del nivel de estigma en población que presente antecedente auto reportado de tuberculosis frente a población que no cuente con este antecedente fue de 1.19; la cual no fue significativa (p=0.089). Conclusión. El presente trabajo no encontró asociación entre los niveles de estigma y el antecedente auto reportado de tuberculosis.
Niveles de presión arterial y su asociación con mortalidad: resultados a largo plazo del estudio PERU MIGRANT
Authors:   ,   Universidad Peruana de Ciencias Aplicadas (UPC), Hidalgo Benites Aida Luz , Senosain León Valeria Melissa
Published: November 2020

Objetivos: En el mundo, la hipertensión arterial es un importante problema de salud pública que afecta a la población, por eso su prevalencia, forma de diagnóstico y tratamiento son evaluados constantemente. El objetivo de este estudio fue el de comparar la mortalidad con niveles de presión arterial superiores al rango normal bajo dos diferentes definiciones, JNC-7 y ACC/AHA 2017. Metodología: Análisis secundario de datos del estudio de cohorte PERU MIGRANT. La exposición de interés fue los niveles de presión arterial clasificados bajo dos diferentes definiciones (JNC-7 y ACC/AHA 2017), mientras que la variable resultado fue mortalidad a 10 años. Se utilizó la prueba de Log Rank, el análisis de Kaplan-Meier y la regresión de riesgos proporcionales de Cox para estimar la asociación entre las variables de interés, ajustado por confusores y reportándose Hazard Ratios (HR) e intervalos de confianza al 95% (IC 95%). Resultados: De los 989 registros analizados, 52,6% eran mujeres, con una media de edad de 48,0 (DE: 12,0). La prevalencia de hipertensos fue de 31,3 % y 16,0% de acuerdo a ACC/AHA 2017 y JNC-7, respectivamente. Los niveles de presión arterial se asociaron a mayor mortalidad usando cualquiera de las dos definiciones. Según la clasificación ACC/AHA 2017, presión arterial elevada, HTA I, HTA II los HR obtenidos fueron: 1,66(0,68-4,07); 2,83(1,23-6,53); 5,09(2,45-10,55), respectivamente. Según JNC-7, pre-hipertensión e hipertensión se asociaron a HR 2,14(1,02-4,54) y 5,08(2,45-10,53), respectivamente. Conclusiones: Mayores niveles de presión arterial estuvieron asociados a un incremento en la mortalidad por cualquier causa usando ambas definiciones.
Caracterización nutricional de un néctar elaborado a partir de lactosuero dulce, y Myrciaria dubai(Camu - Camu) edulcorado con Stevia rebaudiana (Stevia)
Authors:   ,   Universidad Peruana de Ciencias Aplicadas (UPC), Courreges Rivero Karla Alexandra
Published: November 2020

Objetivos: La presente tesis tuvo como objetivo general caracterizar nutricionalmente un néctar elaborado a partir de lactosuero dulce, y Myrciaria dubai (camu - camu) edulcorado con Sstevia rebaudiana (stevia). Materiales y métodos: Fue un estudio experimental de laboratorio, donde se evaluó el efecto de las dosis de lactosuero (30%, 50% y 70%) y pulpa de camu camu (70%, 50% y 30%) sobre sus propiedades nutricionales y sensoriales. Resultados: Los análisis físicoquímicos de las tres formulaciones cumplieron con los estándares de calidad para bebidas de frutas. La evaluación sensorial se realizó a 79 panelistas no entrenados, los cuales determinaron que la formulación que contenía 70% pulpa de camu - camu y 30% lactosuero fue la que presentó el mayor puntaje en la evaluación sensorial siendo solo de 2,5/7 en una escala hedónica, por lo que se recomienda mejorar las formulaciones. Discusión: El análisis físico químico de las bebidas formuladas mostró que el néctar a base de lactosuero con camu camu presentó en 100 gramos de producto un bajo nivel de calorías un promedio de 23 Kcal/ 100g, 5,3% de carbohidratos, 0,4% de proteína, 0,1% de grasa, 93,8% de agua, 0,4% de cenizas. El análisis microbiológico indicó que cumplen con la Norma sanitaria que establece los criterios microbiológicos de calidad para néctares, lo que garantiza la inocuidad del producto. Se realizó el análisis de vitamina C en la pulpa de camu-camu, la mezcla de la bebida antes de la pasteurización y luego de la pasteurización siendo estos: 195,8mg/100g, 180,7 mg/100g, 168,3mg/100g respectivamente observándose que en el producto final existe una disminución de alrededor del 14,04% con respecto al contenido inicial en el camu-camu. Conclusión: Las características nutricionales del néctar elaborado a partir de lactosuero, camu camu edulcorado con Stevia presentó un leve incremento en el valor de proteínas (0,3g/100ml) que los néctares comerciales (0g/100ml) que se expenden en el país, pero destaca por su contenido alto de vitamina C (168,3mg/100ml), sin embargo, la incorporación del lactosuero no resultó en una clara aceptabilidad sensorial en jóvenes por ende se rechaza la hipótesis que el lactosuero aumenta la aceptabilidad sensorial. Asimismo, se acepta la hipótesis que aumenta la calidad nutricional del néctar en comparación con bebidas similares que se expenden en el mercado nacional.
Seroprevalensi Tetelo pada Peternakan Itik di Desa Takmung Kabupaten Klungkung
Authors: Cahyani Ni Luh Risna , Suardana Ida Bagus Kade , Nindhia Tjokorda Sari
Published: November 2020

Itik merupakan salah satu jenis komoditas ternak di Indonesia yang mempunyai peluang untuk dikembangkan sebagai penghasil telur dan daging. Salah satu jenis penyakit yang sering menyerang itik adalah tetelo dimana penyakit ini sangat merugikan bagi industri peternakan unggas. Penyakit ini dapat menyerang ayam, itik dan kalkun dengan gejala klinis tremor, tortikolis, dan inkordinasi. Penyebaran penyakit ini melalui kontak langsung dari itik yang terinfeksi ke itik yang sehat. Penyakit ini telah mewabah diseluruh Indonesia termasuk Provinsi Bali. Seroprevalensi tetelo di Kabupaten Klungkung sekitar 46.2% pada tahun 2015. Penelitian ini bertujuan untuk mengetahui berapa seroprevalensi tetelo pada itik di Desa Takmung, Kabupaten Klungkung tahun 2019. Sampel penelitian adalah serum itik yang diambil dari tiga peternakan di Desa Takmung, Kabupaten Klungkung. Pada setiap peternakan diambil sebanyak 50 sampel. Maka total sampel sebanyak 150 sampel serum itik yang tidak divaksinasi. Serum diuji serologi menggunakan uji hambatan hemaglutinasi (HI) di Laboratorium Virologi Fakultas Kedokteran Hewan, Universitas Udayana. Hasil penelitian menunjukan bahwa di Desa Takmung kabupaten, Kabupaten Klungkung terinfeksi virus tetelo dengan seroprevalensi sebesar 5.33%.
Studi Kasus: Penanganan Hernia Inguinalis pada Anjing Campuran Pomeranian Betina dengan Pembedahan
Authors: Tahalli Tahalli , Dada I Ketut Anom , Wirata I Wayan
Published: November 2020

Hernia inguinalis merupakan protursi dari suatu organ atau bagian dari organ, lemak atau jaringan melalui cincin inguinal, yaitu diantara pangkal paha dan otot perut. Tujuan penulisan studi kasus ini adalah untuk mengetahui cara mendiagnosis, penanganan dan pengobatan kasus hernia inguinalis pada anjing. Seekor anjing campuran Pomeranian berumur tiga tahun dengan berat badan 4 kg, dengan warna rambut putih, berjenis kelamin betina, telah didiagnosis menderita hernia inguinalis dengan prognosis fausta. Metode pengobatan yang dipilih adalah tindakan pembedahan. Sebelum dilakukan pembedahan, anjing kasus diberikan premedikasi menggunakan atropin sulfat 0,03 mg/kg BB dan sebagai anestesi digunakan kombinasi ketamin dan xylazin. Dosis ketamin diberikan 13 mg/kg BB dan xylazin 2 mg/kg BB. Anjing ditangani dengan pembedahan, insisi dilakukan pada kulit dan subkutan tepat di atas cincin hernia hingga terlihat isi hernia. Selanjutnya dilakukan reposisi dengan cara memasukkan isi hernia ke dalam rongga abdomen. Setelah reposisi, pada bagian tepi cincin hernia dibuat luka baru untuk memungkinkan terjadinya penyatuan jaringan. Kemudian dilakukan penjahitan pada peritoneum dengan polyglycolic acid 3.0 dengan pola jahitan terputus sederhana, jahitan subkutan menggunakan catgut 3.0 dengan pola jahitan menerus sederhana dan pada kulit dijahit menggunakan pola jahitan terputus sederhana menggunakan benang silk 3.0. Pasca operasi diberikan antibiotik injeksi amoxicillin 1 ml/10 kg BB yang dilanjutkan dengan pemberian obat peroral yaitu antibiotik amoxicillin 500 mg (20 mg/kg BB) selama tujuh hari, pemberian analgesik meloxicam 7,5 mg (0,2 mg/kg BB) selama lima hari. Satu minggu kemudian anjing dinyatakan sembuh berdasarkan keadaan fisik dan klinis.
Genetic diversity of ten Moroccan populations of Tetraclinis articulata as revealed by Inter Simple Sequence Repeat (ISSR) markers
Authors: MAKKAOUI Meryem , ABBAS Younes , EL ANTRY-TAZY Salwa , MEDRAOUI Leila , ALAMI Mohammed , RABANI Selouka , FILALI-MALTOUF Abdelkarim
Published: November 2020

Tetraclinis articulata (Vahl) Masters is one of Morocco's most important forest species. It is also found occasionally in Malta and Spain, showing significant adaptability to different bio-climatic conditions. However, the species is being affected by anthropogenic fragmentation, logging and neglect from authorities, which could lead to the irretrievable loss of this resource. In this study, the genetic diversity and genetic structure of ten Moroccan populations of T. articulata were assessed. Fifteen Inter-Simple Sequence Repeat (ISSR) markers were used. These generated 271 polymorphic fragments with an average of 18.06 per primer and showed 79.59% of polymorphism. The 129 individuals revealed a high level of genetic diversity (Hs = 0.221; Ht = 0.254) and 85% of genetic variation within populations. However, the genetic differentiation level was low (Gst = 0.13), which is consistent with the lack of correlation between genetic and geographic distances revealed by the Mantel test, resulting in a high level of gene flow (Nm = 3.294). Based on PCoA and neighbour-joining methods, the ten populations clustered under the effect of continental and marine climates. Compared with other conifers, the current genetic diversity and the pattern of T. articulata population structure indicate an important gene pool requiring efficient conservation strategies.
Ege Üniversitesi Tıp Fakültesi Hastanesi’nde COVID-19 vakalarının sürveyansı, filyasyonu ve temaslılarının belirlenmesi
Authors: DURUSOY Raika , ATA Aslı , GEÇİM Ceyda , FİLİS Nurdan , FİDAN Ece , ŞİMŞEK Sera , CEVHERTAŞ Aysun , DİRİM Abdullah , KARAKAŞ Emine , BALCI Berna , DURGUN Kevser , AKTUNA Atalay , DURMAZ Seyfi , SERTÖZ Rüçhan , ERSEL Murat
Published: November 2020

Amaç: Bu çalışmayla Ege Üniversitesi hastanesinin COVID-19 sürveyans, filyasyon ve temaslı işlemlerinde halk sağlığı ekibinin alabileceği rolleri yaşanmış bir örnek üzerinden ortaya koymak, temaslı sağlık çalışanı işlemlerinin organizasyonunu paylaşmak, 18 Mart-31 Mayıs 2020 arasında yapılan çalışmaların temel bulgularını sunmak hedeflenmektedir. Yöntem: Retrospektif tanımlayıcı bir çalışmadır. Pandemi sırasında İl Sağlık Müdürlüğüne hastanenin günlük sürveyans verilerinin gönderilmesi için organize olunmuştur. Hastanede COVID-19 PCR testi pozitif saptanan olguların filyasyon ve temaslı verileri kayıtlardan incelenmiştir. Olguların sosyodemografik özellikleri, risk faktörleri, ilk tanı anında belirtileri gibi özellikleri incelenmiştir. Olguların sağlık çalışanı olan ve olmayan temaslı sayıları, ne kadarının izole edildiği, temaslı sağlık çalışanlarının ne kadarına ilaç başlandığı, PCR testi uygulandığı derlenmiştir ve bu çalışmaların organizasyon deneyimi aktarılmaktadır. Tanımlayıcı istatistikler kullanılmıştır. Sağlık Bakanlığı ve etik kurul onayları alınmıştır. Bulgular: Toplam 595 olgu ile görüşülmüş ve 2618 temaslı bilgisi alınmıştır. Görüşülen olguların %51.3’ü erkek, %67.6’sı 20-59 yaş aralığındadır. Olguların ilk belirti başlangıç tarihi ile ilk PCR pozitifliği arasında geçen süre ortalama 4.4±3.6 gündür, 266’sı (%52.8) ilk 3 gün içinde PCR pozitif saptanmıştır. Olası kaynağı sınıflandırılabilen olguların (n=475) 290’ının (%61.1) ailesi/yakınları/komşuları arasında; 98’inin (%20.6) işyerinde bilinen en az bir COVID-19 olgusu bulunmaktadır. Salgının ilk iki haftasında olguların sırasıyla %62.5 ve %17.1’i tıp fakültesi (TF) çalışanı iken bu oran giderek azalmış ve 11.haftanın sonunda %8.7’dir. TF hastanesinde tanı konan/izlenen olguların fatalite oranı %4.5’tir (27/595). Sonuç: Sağlık Bakanlığı bünyesinde halk sağlığı uzmanlarının da rol aldığı COVID-19 sürveyansı, filyasyonu ve temaslı belirlenmesi işini üniversiteler bünyesinde halk sağlığı öğretim elemanları yapabilmektedir. Yapılan çalışmalar, sürveyans ve salgın kotrolüne katkının yanısıra uzmanlık öğrencilerine deneyim fırsatı oluşturmaktadır.
Stemona fimbritepala (Stemonaceae), a new species from northern Laos
Authors:   Queen Sirikit Botanic Garden, The Botanical Garden Organization, Chiang Mai 50180, Thailand, Rakarcha Sarayut , Pongamornkul Wittaya ,   Queen Sirikit Botanic Garden, The Botanical Garden Organization, Chiang Mai 50180, Thailand, Thammarong Woranart ,   Queen Sirikit Botanic Garden, The Botanical Garden Organization, Chiang Mai 50180, Thailand, Maknoi Charun ,   Ban Romklao Botanic Garden, The Botanical Garden Organization, Bo Phak, Chat Trakan, Phitsanulok 65170, Thailand, Souvannakhoummane Keooudone ,   Centre for Development and Environment, Lao country office, Unit 11, #136, Simuang Road, Hom 1, P.O. Box 1438, Ban Phapo, Vientiane, Laos
Published: November 2020

Stemona fimbritepala (Stemonaceae), a new species from northern Laos, is described and illustrated. It is morphologically similar to S. hirtella and S. kerrii but clearly differs in longer peduncle, longer tepals, longer petaloid outgrowth of the connective and fimbriate margin of inner tepals. A detailed description, distribution, ecology, etymology, preliminary conservation status and photographs of this species are provided. A key to discriminate between the three hairy species is presented.
ESCORPIÕES: UM ESTUDO DE CASO COM ALUNOS DO ENSINO FUNDAMENTAL EM ESCOLAS DOS MUNICÍPIOS DE PORTO NACIONAL-TO
Authors: Nt Gomes Ribeiro Derval , Gomes Corrêa Yara , Simone Seibert Carla
Published: November 2020

A educação ambiental pode ajudar na sensibilização da sociedade em relação aos escorpiões, desmistificando a visão antropocêntrica no qual o indivíduo se põe acima dos animais não humanos e da natureza, bem como a promover uma interação socioambiental mais justa entre ambos e o ambiente. Conhecer o animal não humano em seu habitat e respeita-lo como tal, pode ajudar na preservação das espécies de escorpião, bem como prevenir acidentes. Para melhorar a relação entre ser humano e animais não humanos, foi desenvolvido um trabalho socioambiental com crianças de 11 a 12 anos, por meio de realização de oficinas com apresentação de materiais didáticos sobre os escorpiões. Foi elaborado um questionário contendo oito questões de fácil interpretação e resposta. As perguntas demonstraram valores significativos de dependência do pós teste em relação a oficina, sendo possível dizer que os sujeitos participantes possuem uma visão convencional acerca dos escorpiões, que pode ser modificada com uma interversão didático-metodológica intencional.

 

PALAVRAS-CHAVE: Tityus sp. Sensibilização. Crianças. Interação Socioambiental.
Analysis of Chromium (III)Picolinate in Capsule dosage form
by using Stability indicating HPTLC Method
Authors: Kakade Aditi R. , Yadav Savita
Published: November 2020

Chromium (III) picolinate is used as a nutritional supplement and has been valuable
effects on carbohydrate and lipid metabolism alleviating symptoms associated with diabetes. A
chromium supplement produces beneficial results in reducing insulin sulfonylurea or metformin
requirements. Hence, stability indicating HPTLC method was developed for estimation of
Chromium picolinate in capsule formulation. The development of HPTLC method optimization
on precoated silica gel 60 F254 aluminium plates of 20 cm x 20 cm, 250μm thickness. The
mobile phase used was methanol: ethyl acetate6:4 (v/v). The densitometry detection was done
at 264 nm. Also, the forced degradation studies were performed and method was validated with
as per ICH guidelines. The Rf value obtained was 0.39 ±0.05.Linearity data for the calibration
curve gave a good linear relationship over the concentration range of 100-600ng/spot for
Chromium picolinate (correlation coefficient R2
=0.9997). The percent recovery of Chromium
picolinate in marketed formulation was found in the range of 99.56%.Force degradation and
validation of method was done as per ICH guidelines and the results are within the compliance
limit.The developed HPTLC method gave good results for force degradation studies show that
the method is stability indicating. Thus, this method can be used for routine analysis of
Chromium picolinate and can be use for its future usage
Isolation and Antibacterial Activity of Endophytic Bacteria
Isolates from Gambir (Uncaria gambir (Hunter) Roxb.)
Authors: Ompusunggu Suryani , Suryanto Dwi , Yurnaliza
Published: November 2020

A study on isolation and antibacterial activity of endophytic bacteria of gambir
(Uncaria gambir (Hunter) Roxb.), along with its antibacterial extract activity test on clinical
pathogenic bacteria has been conducted. Isolation of gambir endophytic bacteria was done in
nutrient agar incubated at ambient temperature for 48 hours. Samples of gambir leaves and root
was used for source of endophytic bacteria. Bacterial isolate was propagated using Mueller
Hinton Agar. Bacterial culture was extracted using methanol as solvent. To test methanol
extract activity of bacterial isolates, concentration of 20, 40, 60, 80, and 100% (v/v) were used
in paper disc. Pathogenic bacterial isolates such as Escherichia coli, Streptococcus mutans, and
Staphylococcus aureus were used for antibacterial test. Inhibition zone formed around paper
disc indicated antibacterial extract activity against the bacteria. Endophytic bacterial isolates of
gambirwas identified using Vitek Compact 2.0®
based on biochemical test. Isolates SO02 and
SO03 showed relatively higher antibacterial activity, with inhibition zone of 17 and 30 mm,
respectively. Methanol extract of SO02 showed to have higher activity of >10 mm at 100% of
extract concentration, compared to that of SO03 which only showed <10 mm) against E.
coli.High inhibition against S.mutans and S.aureus was obtained at of 60%. Chemical
identification found that methanol extract of SO02 and SO03 showed to have flavonoid and
tanning group. Bacterial identification showed that SO02 and SO03 were closely related to
Enterobacter cloacae and Gemella morbillorum with similarity of 98 and 86%, respectively.
However, accurate species of these two isolates should be determined
Indigenous Bamboo and Resource Management in Kenya’s Aberdare Forests and Rural Communities
Authors: Sasahara Chika , Shibata Shozo
Published: November 2020

The Kenya Forestry Service permits rental of low-density national forest areas for use as common farmland, but public access to such areas is restricted to prevent deforestation against a background of rising demand for fuel wood. This study was conducted to clarify characteristics relating to the production of indigenous bamboo (a useful wood resource) by the approximately 23% of locals who work on common farmland in central Kenya’s Kamae forest management area, with estimation to determine above-ground biomass. The results showed that bamboo still represents 60% of such biomass, indicating sustainability in the industry.
The Challenge of Evaluating Response to Peptide Receptor Radionuclide Therapy in Gastroenteropancreatic Neuroendocrine Tumors: The Present and the Future.
Authors: Liberini Virginia , Hüllner Martin W. , Grimaldi Serena , Finessi Monica , Thuillier Philippe , Muni Alfredo , Pellerito Riccardo E. , Papotti Mauro , Piovesan Alessandro , Arvat Emanuela , Deandreis Désirée
Published: November 2020

The NETTER-1 study has proven peptide receptor radionuclide therapy (PRRT) to be one of the most effective therapeutic options for metastatic neuroendocrine tumors (NETs), improving progression-free survival and overall survival. However, PRRT response assessment is challenging and no consensus on methods and timing has yet been reached among experts in the field. This issue is owing to the suboptimal sensitivity and specificity of clinical biomarkers, limitations of morphological response criteria in slowly growing tumors and necrotic changes after therapy, a lack of standardized parameters and timing of functional imaging, and the heterogeneity of PRRT protocols in the literature. The aim of this article is to review the most relevant current approaches for PRRT efficacy prediction and response assessment criteria in order to provide an overview of suitable tools for safe and efficacious PRRT.
PENERAPAN TEKNOLOGI BIOPORI UNTUK MENINGKATKAN KETERSEDIAN AIR TANAH SERTA MENGURANGI SAMPAH ORGANIK DI DESA PURON SUKOHARJO
Authors: Arifin Zainal , Tjahjana Dominicus Danardono Dwi Prija , Rachmanto Rendy Adhi , Suyitno Suyitno , Prasetyo Singgih Dwi , Hadi Syamsul
Published: November 2020

Ketersediaan air tanah di Desa Puron Kecamatan Bulu Kabupaten Sukoharjo semakin menurun. Hal itu dikarenakan pengambilan air tanah yang tidak diimbangi dengan semangat konservasi dan sedikitnya air hujan yang masuk ke dalam tanah. Berkurangnya jumlah air yang ada mengakibatkan mengeringnya tumbuhan di lingkungan tersebut. Selain itu, masih banyaknya sampah rumah tangga menjadi permasalahan yang harus dihadapi masyarakat permukiman. Penanganan sampah dan irigasi secara konvensional yang telah diterapkan oleh masyarakat belum menjadi solusi terbaik. Sehingga perlu penanganan secara tersistematis, terstruktur dan berkelanjutan. Pengabdian yang dilakukan bertujuan untuk membantu masyarakat Desa Puron dalam menangani masalah air dan sampah. Pengabdian masyarakat dengan mensosialisasikan dan mengajak masyarakat untuk melakukan konservasi sumber daya air dengan penerapan teknologi sederhana dan murah. Penerapan teknologi yang bisa diterapkan yaitu dengan pembuatan lubang resapan biopori untuk meningkatkan jumlah air dalam tanah serta mengurangi sampah organik di Desa Puron. Lubang resapan biopori berfungsi sebagai tempat resapan air ke dalam tanah dan sebagai tempat pembuatan kompos dari sampah organik. Lubang resapan biopori tidak membutuhkan area luas dan proses pembuatannya sangat mudah, hal ini tentu menjadi solusi yang tepat untuk wilayah Desa Puron. Hasil pengabdian menjadikan ketersedian air yang ditandai dengan menghijaunya tumbuhan.

 

Kata kunci: Air, Biopori, Pengabdian, Desa Puron
Sales Quality Determination Using Simple Additive Weighting (SAW) and Analytical Hirarki Process (AHP) Methods
Authors: Khozaimi Ach , Pramudita Yoga Dwitya , Sari Rochman Eka Mala , Rachmad Aeri
Published: November 2020

Salt has a salty taste obtained by drying seawater into mineral crystals. However, the quality of industrial salt in Indonesia is still an obstacle, so the need for industrial salt still relies on imported salt. The quality of salt is shown from physical and chemical appearance. Good salt is shown by the crystal form which is smooth and has a clear white color. Good salt quality knowledge must be known so that it can meet Indonesia's national standards and can distinguish salt designation based on its quality category. This study compares the Simple Additive Weighting (SAW) and Analytical Hierarchy Proccess (AHP) methods to determine the highest quality salt. The SAW method gives weight to each of its attributes and the total score finally produces an alternative decision that matches the criteria, thus producing a high-quality salt. While the AHP method starts by creating a hierarchical structure that has the main objectives, criteria, sub criteria and alternatives to be discussed. Pairwise comparisons are used to form relationships in the structure. So it will form a matrix where the ratio scale is derived in the form of the main vector eigen or function-eigen.
Optimization of Daylight Factor Distribution Using Standard Deviations Based on Shifting Window Position
Authors: Rizal Yose , Robandi Imam , Yuniarno Eko Mulyanto
Published: November 2020

Natural lighting is an important factor that affects the comfort of building users. Natural lighting in a room requires a window area of ​​at least 1/6 of the floor area. This study was conducted to obtain the distribution of Daylight Factor (DF) as a natural lighting factor during the day in the room, based on the shift in the position of the window on the wall. The distribution of lighting entering the depth of the room through window openings is a tool to compare the best window position in the spread of illumination with DF calculations based on Sky Component (SC). Shifting the window position will be analyzed by Standard Deviation (S) and Mean (μ) based on the DF distribution. Optimizations of the DF distribution on the window position shifts if it has the largest DF mean value and the smallest DF variant value. The results of the study in a simple room showed that the optimal DF distribution was at the window position in the middle and the mean value was 2.59%. The relationship of shifting window position and DF distribution can be useful for architects to determine the function of a room in architectural design.
Wheatstone and Iago Fields Interference Testing: Execution and Interpretation with Ensemble Variance Analysis
Authors: Flett Matthew , Connell Paul
Published: November 2020

The Wheatstone Project is an LNG development located in Western Australia, incorporating the Chevron Australia operated Wheatstone and Iago fields and third-party gas fields. Production commenced in mid-2017. The start-up of the Wheatstone and Iago fields was a unique opportunity to acquire reservoir surveillance information that was able to provide valuable reservoir insights. A key component of the analysis was the observation of pressure interference between wells from the initial well clean-ups. The design of the well clean-up sequence, discussed in a previous paper (Flett & Connell, 2019) was influenced by operational guidelines and the application of Ensemble Variance Analysis to determine an optimised start-up strategy for interference testing with the objective of reducing subsurface uncertainties.

The well clean-up sequence was executed safely and achieved the reservoir surveillance and operational objectives of the start-up program. Flexibility in the optimised well clean-up sequence allowed changes to the start-up strategy in response to changing operating conditions, without impacting the reservoir surveillance objectives.

High quality pressure interference data was acquired, enabling clear determination of zonal reservoir connectivity within the Wheatstone and Iago reservoirs. Interpretation of pressure interference results with reservoir models, incorporating Ensemble Variance Analysis led to a change in approach for initial history matching and revision of static and dynamic model development for Wheatstone and Iago fields.
Genome-wide association studies for methane emission and ruminal volatile fatty acids using Holstein cattle sequence data
Authors: Sarghale Ali Jalil , Shahrebabak Mohammad Moradi , Shahrebabak Hossein Moradi , Javaremi Ardeshir Nejati , Saatchi Mahdi , Khansefid Majid , Miar Younes
Published: November 2020

Background: Methane emission by ruminants has contributed considerably to the global warming and understanding the genomic architecture of methane production may help the livestock producers to reduce the methane emission from the livestock production system. The goal of our study was to identify genomic regions affecting the predicted methane emission (PME) from volatile fatty acids (VFAs) indicators and VFA traits using imputed whole-genome sequence data in Iranian Holstein cattle.Results: Based on the significant-association threshold (p < 5 × 10−8), 33 single nucleotide polymorphisms (SNPs) were detected for PME per kg milk (n=2), PME per kg fat (n=14), and valeric acid (n=17). Besides, 69 genes were identified for valeric acid (n=18), PME per kg milk (n=4) and PME per kg fat (n=47) that were located within 1 Mb of significant SNPs. Based on the gene ontology (GO) term analysis, six promising candidate genes were significantly clustered in organelle organization (GO:0004984, p = 3.9 × 10-2) for valeric acid, and 17 candidate genes significantly clustered in olfactory receptors activity (GO:0004984, p = 4 × 10-10) for PME traits. Annotation results revealed 31 quantitative trait loci (QTLs) for milk yield and its components, body weight, and residual feed intake within 1 Mb of significant SNPs.Conclusions:Our results identified 33 SNPs associated with PME and valeric acid traits, as well as 17 olfactory receptors activity genes for PME traits related to food preference and feed intake. Identified SNPs in this study were close to 31 QTLs for milk yield and its components, body weight, and residual feed intake traits. In addition, these traits had high correlations with PME trait. Overall, our findings suggest that marker-assisted and genomic selection could be used to improve the difficult and expensive-to-measure phenotypes such as PME. Moreover, prediction of methane emission by VFA indicators could be useful for increasing the size of the reference population required in genome-wide association studies and genomic selection.
Cost-Effectiveness Analysis Of Text Messaging To Support Health Advice For Smoking Cessation 
Authors: Cobos-Campos Raquel , Mar Javier , Apiñaniz Antxon , de Lafuente Arantza Sáez , Parraza Naiara , Aizpuru Felipe , Orive Gorka
Published: November 2020

Background: Smoking in one of the most serious public health problems. It is well known that it constitutes a major risk factor for chronic diseases and the leading cause of preventable death worldwide.Due to high prevalence of smokers, new cost-effective strategies seeking to increase smoking cessation rates are needed. Methods: We performed a Markov model-based cost-effectiveness analysiscomparing two treatments: health advice provided by general practitioners and nurses in primary care, and health advice reinforced by sending motivational text messages to smokers’mobile phones. A Markov model was used in which smokerstransitioned between three mutually exclusive health states (smoker, former smoker and dead) after 6-month cycles. We calculated the cost-effectiveness ratio associated with the sending of motivational messages. Health care and society perspectives (separately) was adopted. Costs taken into account were direct health care costs and direct health care cost and costsfor lost productivity, respectively.Additionally, deterministic sensitivity analysis was performed modifying the probability of smoking cessation with each option. Results: Sending of text messages as a tool to support health advice was found to be cost-effective as it was associated with increases in costs of €7.4 and €1,327 per QALY gained (ICER)for men and women respectively from a healthcare perspective, significantly far from the published cost-effectiveness threshold. From a societal perspective, the combined programmed was dominant. Conclusions: Sending text messages is a cost-effective approach. These findings support the implantation of the combined program across primary care health centres.
Nasal Nalbuphine Analgesia in Prehospital Trauma Management by First-Responder Personnel on Ski Slopes in Switzerland
Authors: Pietsch Urs , Berger Yoël , Schurter David , Theiler Lorenz , Wenzel Volker , Meuli Lorenz , Grünenfelder Andreas , Albrecht Roland
Published: November 2020

Background Pain is one of the major symptoms complained about by patients in the prehospital setting, especially in trauma. Due to the mountainous topography, there may be a time delay between injury and arrival of professional rescuers, in particular on ski slopes. Administration of a safe opioid by first responders (FR) may improve overall treatment. We therefore assessed the use of nasally administered Nalbuphine as analgesic treatment in trauma patients by FR at various skis resort in Switzerland.Methods This observational study examined a cohort of 267 patients given Nalbuphine by FR in various ski resorts in Switzerland. All FR were instructed how to administer Nalbuphine before treating patients. A treatment algorithm was developed and distributed to assure that Nalbuphine was only administered following a strict protocol. Data regarding pain scores and pain reduction after administration of Nalbuphine were collected on-site. Refills were handed out with each completed questionnaire.Results Nalbuphine decreased the level of pain statistically significant and clinically relevant by a median of 3 units on the NRS for pain. The multivariate regression model showed that pain reduction was more pronounced in patients with higher initial pain level. Nalbuphine was less effective in patients aged 20-60 years compared to the adolescent population. No major side effects were observed.Conclusion Nasal Nalbuphine by FR was a noninvasive pain management strategy that provided safe and effective analgesia in prehospital, acute­ly injured patients. This may be an alternative method especially in circumstances of severe pain and prolonged time between arrival of the FR and arrival of EMS / HEMS personnel on scene.
Coronin 1A Expression in Human Astroglia, its Function in Physiology and Astrogliosis in HIV-1 Neuropathogenesis
Authors: Pandey Hriday Shanker , Kapoor Rishabh , Bindu  , Seth Pankaj
Published: November 2020

In most neurodegenerative disorders, including neuroAIDS, reactive astroglia are detrimental to the neuronal population. Calcium and its downstream regulators play a central role in mediating glial activation. Coronin 1A, an acting binding protein, majorly reported in cells of hematopoietic origin, regulates cell activity in a calcium-dependent manner, but its role in astroglial physiology and astrogliosis is largely unknown. Using a well-characterized primary culture of human astroglia and neurons, we explored the roles of Coronin 1A in astroglia physiology and the mechanisms by which it facilitates reactive astrogliosis. In this study, we report for the first time, that human primary astroglia express Coronin 1A, and it plays activity-dependent roles in events such as PLCγ1 phosphorylation followed by Calcium mobilization from the intracellular stores. HIV-1 Tat, a potent neurotoxicant that induces astrogliosis, enhances the expression of Coronin 1A, apart from affecting GFAP and pro-inflammatory molecules. Downregulation of Coronin 1A ameliorated the HIV-1 Tat-induced deleterious effects of reactive astroglia, measured as enhanced GFAP expression and release of IL-6, and Glutamate and thus reduced glia-mediated neurodegeneration. Our findings also suggest that out of a pool of dysregulated miRNAs studied by us, hsa-miR-92b-5p regulates Coronin 1A expression which further facilitates reactive astrogliosis under the effect of HIV-1 Tat. These findings highlight the novel roles of Coronin 1A in regulating the astroglial physiology and astrogliosis observed in HIV-1 neuropathogenesis.
Molecular surveillance of pfcrt, pfmdr1 and K13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China
Authors: Huang Fang , Yan He , Xue Jing-Bo , Cui Yan-Wen , Zhou Shui-Sen , Xia Zhi-Gui , Abeyasinghe Rabindra , Ringwald Pascal , Zhou Xiao-Nong
Published: November 2020

Background
The emergence and spread of multidrug resistance pose a significant risk to malaria control and eradication goals in the world. There has been no indigenous malaria cases reported since 2017, and China is approaching malaria elimination by 2020. Therefore, it is urgent to monitor antimalarial drug resistance and track the emergence and spread of the imported drug resistant malaria cases in China.
Methods
Dried blood samples were obtained from P. falciparum infected cases who returned from Africa to China between 2012–2015 prior to antimalarial drug treatment. The known polymorphisms relating to drug resistance of pfcrt, pfmdr1 gene, and the propeller domain of the K13 gene were evaluated by nested PCR and sequencing. The GraphPad prism was used to plot the prevalence of each mutation. The chi-squared test and two-sided P value of < 0.05 were used to evaluate differences with statistical significance.
Results
A total of 731 P. falciparum isolates were successfully genotyped at the pfcrt locus. The wild type haplotype of C72V73M74N75K76 was the most prevalent genotype with the prevalence of 62.8% and 29.8% of the isolates showed the triple mutant haplotype C72V73I74E75T76. Total 434 P. falciparum isolates were successfully sequenced and pfmdr1 allelic variants were only observed in codons 86, 184, and 1246. Twelve haplotypes were identified and the prevalence of the wild type variant pfmdr1 N86Y184D1246 was 44.1%. The single mutant pfmdr1 in codons 86 and 184 was predominant but D1246Y was rare. The double mutant genotype Y86F184D1246 was common in Africa. A total of 1381 P. falciparum isolates from 33 African countries were sequenced of K13-propeller domain and 1357 were successfully sequenced. The prevalence of K13 mutations was 3.6% and 26 different mutant alleles including 22 nonsynonymous and four synonymous variants (C469C, R471R, G496G, and A627A) were observed. The A578S was the most common haplotype of K13. Three mutation associated with artimisinin resistance (M476I, R539T, and P553L) were observed in three isolates.
Conclusion
The prevalence of mutant pfcrt and pfmdr1 was low or moderate in P. falciparum isolates imported from Africa to China between 2012–2015. K13-propeller mutations were highly diverse but most mutations were only found in a few isolates. This study provides evidence for the antimalarial drug resistance level of the imported malaria cases from Africa and the efficacy of antimalarial drug policy in China to treat these imported cases.
LncRNA HAND2-AS1 Exerts Anti-Oncogenic Effects on Bladder Cancer via Restoration of RARB as A Sponge of microRNA-146
Authors: Shan Liping , Liu Wei , Zhan Yunhong
Published: November 2020

Background Growing evidence has shown that the association of long noncoding RNA/microRNA/mRNA is implicated in tumor initiation, development, and progression. Long noncoding RNA (lncRNA) HAND2-AS1 exhibits anti-cancer effects in diverse cancers. However, the knowledge of HAND-AS1 in bladder cancer development remains unknown. Methods LncRNA and miRNA microarray were conducted to explore different expressed RNA in primary bladder cancer specimens. RNA-RNA interaction prediction tools miRcode (http://www.mircode.org/), DIANA-lncBase v2 (https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=lncbasev2%2Findex-experimental), DIANA-TarBase v.8 (https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=tarbasev8%2Findex) and miRDB (http://www.mirdb.org/) were employed to predict the interactions between RNA. Bladder cancer cell lines were used to perform cell proliferation and apoptosis assays. Western blot and quantitative Real-time Polymerase Chain Reaction were used to determine the expression of protein and RNA, separately. Dual luciferase assay was conducted to determine the activity of 3’untranslational region of RARB. Furthermore, 5637 human bladder cancer mouse models were established to investigate the interactions of lncRNA: miRNA: mRNA in vivo.Results Based on the analysis of RT2 lncRNA PCR Arrays, we validated HAND2-AS1 declined in bladder cancer and correlated with the depth of invasion and grades negatively. Overexpression of HAND2-AS1 in human bladder cancer cells 5637 and RT4 hampered cell proliferation by provoking Caspase 3-triggered cell apoptosis. Besides, one of the HAND2-AS1 sponge, miR-146, upregulated in bladder cancer and targeted the tumor suppressor, retinoic acid receptor beta (RARB). We further demonstrated the HAND2-AS1: miR-146: RARB complex promoted Caspase 3-mediated apoptosis by suppressing COX-2 expression. Finally, results observed in mouse xenografts suggested that HAND2-AS1 diminished miR-146 expression, thereby reversing the suppression of miR-146 on RARB-mediated apoptosis and contributing to bladder cancer regression. Conclusion The present study shed light on the fact that lncRNA HAND2-AS1 exerted as a tumor suppressor by releasing RARB from miR-146, leading to inhibition of tumor proliferation and invasion. The findings expanded the knowledge of HAND2-AS-mediated regulatory networks, provided novel insights to improve the RARB-targeted regimens against bladder cancer.
Clinical and Prognostic Implications of an Immune-Related Risk Model Based on TP53 Status in Lung Adenocarcinoma
Authors: Song Xuming , Chen Qiang , Wang Jifan , Mao Qixing , Xia Wenjie , Xu Lin , Jiang Feng , Dong Gaochao
Published: November 2020

Background: TP53 mutation is the most widespread mutation in lung adenocarcinoma (LUAD), meanwhile p53 (encoded by TP53) has recently been implicated in immune responses. However, it is still unknown whether TP53 mutation may remodel tumor microenvironment to influence tumor progression and prognosis in LUAD.Methods: we developed a six-gene immune-related model (IRM) to predict the survival of patients with LUAD in TCGA cohort based on TP53 status using LASSO Cox analysis, which was also confirmed the predictive ability in two independent cohorts.Results: The mutation of TP53 led to a decrease in the immune response in LUAD. Further analysis revealed that patients in the high-index group had observably lower relative infiltration of memory B cells and regulatory T cells, together with significantly higher relative infiltration proportions neutrophils and resting memory CD4+ T cells. Additionally, the IRM index positively correlated with expression of critical immune checkpoint genes including PDCD1 (encoding PD-1) and CD274 (encoding PD-L1), which was validated in Nanjing cohort. Furthermore, the IRM index as an independent prognostic factor was used to establish a nomogram for clinical application.Conclusion: This immune-related model may serve as a powerful prognostic tool to further optimize immunotherapies for LUAD.
Identification the Roles of Ferroptosis-Related Gene Signatures in Patients with Esophageal Adenocarcinoma
Authors: Zhu Lei , Yang Fugui , Wang Lingwei , Dong Lin , Huang Zhiyuan , Wang Guangxue , Chen Guohan , Li Qinchuan
Published: November 2020

Background Ferroptosis is a recently recognized non-apoptotic cell death that is distinct from the apoptosis, necroptosis and pyroptosis. Considerable studies have demonstrated ferroptosis is involved in the biological process of various cancers. However, the role of ferroptosis in esophageal adenocarcinoma (EAC) remains unclear. The aim of this study was to explore the ferroptosis-related genes (FRG) expression profiles and their prognostic values in EAC.Methods The FRG data and clinical information were downloaded from The Cancer Genome Atlas (TCGA) database. Univariate and multivariate cox regressions were used to identify the prognostic FRG, and the predictive ROC model was established using the independent risk factors. GO and KEGG enrichment analyses were performed to investigate the bioinformatics functions of significantly different genes (SDG) of ferroptosis. Additionally, the correlations of ferroptosis and immune cells were assessed through the single-sample gene set enrichment analysis (ssGSEA). Finally, significantly different genes were verified in our clinical EAC specimens and normal esophageal mucosal tissues.Results: Twenty-eight significantly different FRG were screened from 78 EAC and 9 normal tissues. GO and KEGG enrichments showed these SDG were mainly related to the iron-related pathways and metabolisms of ferroptosis. Gene network demonstrated the TP53, G6PD, NFE2L2 and PTGS2 were the hub genes in the biology of ferroptosis. Cox regression analyses demonstrated four FRG (CARS1, GCLM, GLS2 and EMC2) had prognostic values for overall survival (OS) (all P<0.001). ROC curves showed better efficacy to predict survival using the risk score (AUC=0.744). Immune cell enrichment analysis demonstrated that the types of immune cells and their expression levels in the high-risk group were significant different with those in the low-risk group (all P<0.05). The experimental results confirmed the ALOX5, NOX1 were upregulated and the MT1G was downregulated in the EAC tissues compared with the normal esophageal mucosal tissues (all P<0.05).Conclusions: We identified differently expressed ferroptosis-related genes that may involve in the process in EAC. These genes have significant values in predicting the patients’ OS and targeting ferroptosis may be an alternative for therapy. Further studies are necessary to verify these results of our study.
Adverse Effects of Cell Free and Concentrated Ascites Reinfusion Therapy (CART) for Malignant Ascites: A Single Institute Experience
Authors: Tsubokura Misato , Adegawa Yuko , Kojima Minoru , Tanosaki Ryuji , Otake Ryuzaburo , Kase Yuki , Iwashita Nao , Kasane Moemi , Nakabayashi Saori , Takeuchi Sayaka , Kato Ken , Boku Narikazu , Kanemitsu Yukihide , Okusaka Takuji , Fujimoto Hiroyuki , Yonemori Kan , Ishiki Hiroto , Kawamura Kimihiko , Satomi Eriko , Matsushita Hiromichi
Published: November 2020

Background: Cell-free and concentrated ascites reinfusion therapy (CART) is a strategy to improve hypoalbuminemia due to refractory ascites. CART has recently been applied in the treatment of cancer patients. This study was performed to assess the safety of CART in a single cancer institute.Methods: We retrospectively reviewed 233 CART procedures that were performed for 132 cancer patients in our institute. Results: The median volume of ascites before and after concentration was 4,720 and 490 mL (median concentration rate, 10.0-fold), The median amounts of total protein and albumin were 64.0g and 32.6g (median recovery rates, 44.9% and 49.0%), respectively. Thirty-three adverse events (AEs) were observed in 22 (9.4%) of 233 procedures; 30 of these events occurred after reinfusion. The most common reinfusion-related AEs were fever (13 events) and chills (10 events). Univariate analyses revealed no significant relationships between the frequency of AEs and age, sex, appearance of ascites, volume of harvested and concentrated ascites, the ascites processing rate (filtration and concentration), volume of saline used for membrane cleaning, amount of calculated total protein for infusion, or prophylaxis against AEs; the reinfusion rate >100 mL/h affected the frequency of AEs, regardless of the prophylactic use of steroids. Conclusions: The observed AEs were mainly mild cytokine-related reactions after reinfusion, which were related to a reinfusion rate of >100 mL per hour. Although our study was retrospective in nature and undertaken in a single institute, this information may be helpful for the management of cancer patients with refractory malignant ascites using CART.