bluuebunny/crossref_metadata_2025 huggingface dataset:
Drift-Alfvén fluctuations and transport in multiple interacting magnetized electron temperature filaments
Authors: Sydora R. D. , Karbashewski S. , Van Compernolle B. , Poulos M. J. , Loughran J.
Published: December 2019

The results of a basic electron heat transport experiment using multiple localized heat sources in close proximity and embedded in a large magnetized plasma are presented. The set-up consists of three biased probe-mounted crystal cathodes, arranged in a triangular spatial pattern, that inject low energy electrons along a strong magnetic field into a pre-existing, cold afterglow plasma, forming electron temperature filaments. When the three sources are activated and placed within a few collisionless electron skin depths of each other, a non-azimuthally symmetric wave pattern emerges due to interference of the drift-Alfvén modes that form on each filament’s temperature gradient. Enhanced cross-field transport from chaotic (

$\boldsymbol{E}\times \boldsymbol{B}$

, where

$\boldsymbol{E}$

is the electric field and

$\boldsymbol{B}$

the magnetic field) mixing rapidly relaxes the gradients in the inner triangular region of the filaments and leads to growth of a global nonlinear drift-Alfvén mode that is driven by the thermal gradient in the outer region of the triangle. Azimuthal flow shear arising from the emissive cathode sources modifies the linear eigenmode stability and convective pattern. A steady-current model with emissive sheath boundary predicts the plasma potential and shear flow contribution from the sources.
Constructing and negotiating social participation in old age: experiences of older adults living in urban environments in the United Kingdom
Authors: Woolrych Ryan , Sixsmith Judith , Fisher Jenny , Makita Meiko , Lawthom Rebecca , Murray Michael
Published: December 2019

The age-friendly cities and communities movement has focused on how to better support older adults to age well within urban environments. Central to ‘ageing well’ and ‘active ageing’ agendas is ensuring that older adults can participate in meaningful forms of social participation. The benefits of social participation in old age have been well documented, and research amongst community-dwelling older adults has explored some of the neighbourhood qualities that facilitate or impede such forms of engagement. However, understandings of how older adults construct and negotiate social participation within everyday urban environments have been largely unexplored. To address this gap, we present results from 104 interviews conducted with older adults living in three cities and nine neighbourhoods in the United Kingdom (UK). The findings explore three themes generated from the research: ‘constructing meaningful social participation in old age’, ‘negotiating access to social participation’ and ‘navigating home and community’. Across these themes, the paper describes how experiences of social participation in old age involve a number of inter-connected physical, psychological and social processes experienced by individuals across a range of environmental settings including the home, outdoor spaces and community facilities. The paper concludes by discussing the implications of the findings for practice, specifically in the delivery of age-friendly communities.
Work–family lifecourses and later-life health in the United Kingdom
Authors: Tosi Marco , Grundy Emily
Published: December 2019

Socio-economic inequalities in physical and mental health persist at older ages and previous studies have shown that partnership and parenthood histories are also associated with differentials in later-life health. These domains of adult life interact, and both may be influenced by earlier life circumstances, indicating a need for a holistic approach to understanding lifecourse influences on health at older ages. In this paper, we identify classes of lifecourse types for a United Kingdom (UK) cohort born 1933–1945 and investigate differences between the latent classes identified in physical and mental health, and changes in health over a five-year follow-up period. Data were drawn from Waves 1–5 (2009–2013) of the nationally representative UK Household Longitudinal Study. Multi-level models were used to analyse associations with summary indicators of physical and mental health measured using the SF-12, and changes in health, controlling for childhood circumstances and taking account of support from family and friends in later life. Lifecourses characterised by lower socio-economic position, early parenthood and large family size were associated with worse physical and mental health in later life, with respondents who had combined a high socio-economic position and two children being the most advantaged. The study indicates that socio-economic disparities in later-life health vary depending on the way in which individuals combine work and family life.
Optimal input for language learning: Genuine, simplified, elaborated, or modified elaborated?
Authors: Long Michael H.
Published: December 2019

Discussions of optimal types of spoken and written input for language learning have traditionally focused on the relative merits of authentic and linguistically simplified spoken and written texts. I will argue that elaborated input and, in particular, modified elaborated input, constitute better options, with tasks, not just texts, functioning as important input sources. Modified elaborated input, potentially coupled with bimodal presentation, has many positive features, especially, but not only, for programs seeking to increase students' opportunities for enhanced incidental learning.
The landscape-scale drivers of herbivore assemblage distribution on the central basalt plains of Kruger National Park
Authors: Young Cyanne , Fritz Hervé , Smithwick Erica A.H. , Venter Jan A.
Published: December 2019

The distribution and abundance of herbivores in African savannas are constrained by interactions between abiotic and biotic factors. At the species-level, herbivores face trade-offs among foraging requirements, vegetation structure and the availability of surface water that change over spatial and temporal scales. Characterizing herbivore requirements is necessary for the management of the environment in which they occur, as conservation management interventions such as fencing and artificial water provision consequently have effects on how herbivores address these trade-offs. We tested the effects of environmental attributes on the probability of presence of herbivore functional types at different distances to water in the Satara section of Kruger National Park over the period of a year. Hypotheses about species’ relative distribution and abundance were developed through a literature review of forage and water availability constraints on feeding preference and body size of herbivore. We expected strong seasonal relationships between vegetation biomass and quality, and biomass of water-dependent herbivores with increasing distance to water. Our analyses of herbivore distribution across the region confirmed broad-scale descriptions of interactions between forage requirements and water availability across a set of species which differ in functional traits.
A field experiment to determine the effect of dry-season irrigation on vegetative and reproductive traits in the wet-deciduous tree Bonellia nervosa
Authors: Sánchez Octavio , Quesada Mauricio , Dirzo Rodolfo , Schlichting Carl D.
Published: December 2019

Seasonally dry tropical forests (SDTFs) stand out by the diversity of phenological patterns used by plants to deal with dry periods. Although the predominant phenological pattern is dry deciduousness, in Mesoamerican SDTFs the heliophilous tree species Bonellia (formerly Jacquinia) nervosa displays an unusual inverted leaf phenology, producing and holding leaves through the dry season while becoming deciduous in the rainy season. Applying a dry season irrigation field experiment (no water, low watering, high watering), we studied the consequences of contrasting water availability from a phenological plasticity response perspective. Contrary to our expectations, our results show no effect of irrigation treatment on leaf phenology. In addition, mid-day twig water potential showed no significant differences across treatments, but reproductive phenological responses varied among treatments: canopy flowering per cent decreased gradually until the beginning of the wet season in all treatments; meanwhile canopy fruit per cent showed a significant decline under low irrigation. Finally, non-structural carbohydrate concentration (starch) was significantly higher in the high irrigation treatment. Our results showed that inverted leaf phenology remains unaffected regardless of supplemental water availability, and suggest a reallocation of non-structural carbohydrates to fruits and seeds in high-irrigation treatments. Given the current and expected increase in extreme drought events, investigations on the responses of trees of different phenologies, including those of inverted leafing such as Bonellia nervosa, are warranted.
A NEW LOOK ON THE SHORTEST QUEUE SYSTEM WITH JOCKEYING
Authors: Ravid Rachel
Published: December 2019

We introduce a Markov queueing system with Poisson arrivals, exponential services, and jockeying between two parallel and equivalent servers. An arriving customer admits to the shortest line. Every transition, of only the last customer in line, from the longer line to the shorter line may accompanied by a certain fixed cost. Thus, a transition from the longer queue to the shorter queue occurs whenever the difference between the lines reaches a certain discrete threshold (d = 2, 3, …). In this study, we focus on the stochastic analysis of the number of transitions of an arbitrary customer.
Drafting Route Plan Templates for Ships on the Basis of AIS Historical Data
Authors: Naus Krzysztof
Published: December 2019

The paper provides a description of a method of drafting route plan templates on the basis of AIS (automatic identification system) historical data. The first section features a brief background on the problem of drafting route plan templates in the light of international regulations. The main section contains a description of the methods and tools used for processing AIS data into a GRID reference system: ship traffic intensity, average COG (course over ground) and average SOG (speed over ground) as well as route plan templates. The final section includes a presentation of the research method and an analysis of the results, conducted on the basis of maps with charted paths of drafted route plan templates. The summary constitutes a synthesis of general conclusions, the advantages and disadvantages of the solution as well as areas for further research to enhance the solution.
Cultural Expertise in Iran: From the Pahlavi Dynasty to Contemporary Diasporas
Authors: Marin Soudabeh
Published: December 2019

This paper suggests the use of cultural expertise as encompassing concept that can account for the natural cultural competence developed in Iran. In earlier times, Zoroastrian law was first based on religious norms and the primary theological division between sins and offenses. Iranians had to adapt to different legal systems: customary law, religious law and secular law. Priests, jurists, judges, officials and translators were the main cultural “experts” and mediators between the people and the normative institutions. The introduction of imami legal theories and jurisprudence in the 16th century together with the reinforcement of the secular political power engaged Iran in a stabilized judicial context ruled by shiʿi scholars, qāzis and mujtahids. In 1919, as a consequence of the new French inspired Constitution, the Ministry of Justice, in order to train a new generation of judges, magistrates and justice personnel, set up a law school. Professors, who can be considered as cultural experts, contributed to the acculturation process initiated in the judicial system. Examples of his continuous struggle are recounted in Ostad Elahi's (1895-1974) memoirs, relating the difficulty Iranians had to accept the change, both cultural and psychological, initiated by the modernization and westernization program put into operation (1911-1935).
National Sovereignty, European Integration and Domination in the Eurozone
Authors: Maris Georgios
Published: December 2019

The main question this article seeks to answer is: who governs who within the EU? To provide an answer, we will analyse the relationship between national sovereignty and the ability to exercise independent economic policy within the EMU, as well as re-examine the development of this relationship regarding the process of European integration. This research offers a complementary study to understand better the relationship between European integration and national sovereignty.
Heterogeneity of the European Union’s Strategic Partners: Can They Still be Compatible?
Authors: Rivera Pablo Podadera , Garashchuk Anna
Published: December 2019

This article paper aims to investigate the EU’s strategic partners using both theoretical and empirical analysis. Applying the technique of cluster analysis has allowed us, first, to demonstrate that not all of the EU’s ‘special ten’ are strategically sound for the EU; second, to investigate which regional organizations represent the best interest of the EU from a strategic standpoint; third, to find out the true potential of the EU’s strategic partners; and, finally, to prove empirically that the EU’s strategic partners are so heterogeneous as to represent a collective response to multilateralism and that a bilateral approach should be applied instead, taking into consideration the specific character of every strategic partner.
Entirely innocent: a historical sociopragmatic analysis of maximizers in theOld Bailey Corpus
Authors: CLARIDGE CLAUDIA , JONSSON EWA , KYTÖ MERJA
Published: December 2019

Based on an investigation of theOld Bailey Corpus, this article explores the development and usage patterns of maximizers in Late Modern English (LModE). The maximizers to be considered for inclusion in the study are based on the lists provided in Quirket al.(1985) and Huddleston & Pullum (2002). The aims of the study were to (i) document the frequency development of maximizers, (ii) investigate the sociolinguistic embedding of maximizers usage (gender, class) and (iii) analyze the sociopragmatics of maximizers based on the speakers’ roles, such as judge or witness, in the courtroom.

Of the eleven maximizer types focused on in the investigation,perfectlyandentirelywere found to dominate in frequency. The whole group was found to rise over the period 1720 to 1913. In terms of gender, social class and speaker roles, there was variation in the use of maximizers across the different speaker groups. Prominently, defendants, but also judges and lawyers, maximized more than witnesses and victims; further, male speakers and higher-ranking speakers used more maximizers. The results were interpreted taking into account the courtroom context and its dialogue dynamics.
Design and fabrication of a novel single-layer Ka-band reflectarray antenna
Authors: Abdollahvand M. , Forooraghi K. , Encinar Jose A. , Atlasbaf Z. , Martinez-de-Rioja E.
Published: December 2019

A novel dual-polarization, single-layer reflectarray has been designed and manufactured to operate at receive (20 GHz) and transmit (30 GHz) frequencies for Ka-band terminal antennas. The reflectarray unit cell is composed of several types of resonant elements printed on the upper side of a conductor-backed substrate, which are designed to produce a collimated beam at 20 and 30 GHz in dual polarization. Cross-shaped loops are used to provide the required phases at 20 GHz, while crossed dipoles and modified truncated rings are used to control the phasing at 30 GHz. The resonant lengths of the proposed elements have been adjusted cell by cell by means of a two-dimensional interpolation method to achieve the required phase shift at each frequency. Two different feeds have been used to illuminate the reflectarray at 20 and 30 GHz. The measured gain is 28.02 dBi at 20 GHz and 32.14 dBi at 30 GHz. The measurement results show that the radiation patterns of the designed single-layer reflectarray antenna are in good agreement with those achieved from the simulations.
Prediction of Anger Expression of Individuals with Psychiatric Disorders using the Developed Computational Codes based on the Various Soft Computing Algorithms
Authors: Moghadasin Maryam
Published: December 2019

Anger is defined as a psychobiological emotional state that consists of feelings varying in intensity from mild irritation or annoyance to intense fury and rage. Dysfunction in anger regulation is marker of most psychiatric disorders. The most important point about anger regulation by the individuals is how to express anger and control it. The purpose of the present study is to predict the anger expression from the anger experience in individuals with psychiatric disorder for assessment of how to express and control the anger. To this end, the number of 3,000 subjects of individuals with clinical disorders had filled in the State-Trait Anger Expression Inventory–II (STAXI–II). After removing the uncertain diagnoses (900 subjects), the number of 2,100 data was considered in the analysis. Then, the computational codes based on three soft computing algorithms, including Radial Basis Function (RBF), Adaptive Neuro-Fuzzy Inference System (ANFIS) and Decision Tree (DT) were developed to predict the scales of anger expression of the individuals with psychiatric disorders. The scales of anger experience were used as input data of the developed computational codes. Comparison between the results obtained from the DT, RBF and ANFIS algorithms show that all the developed soft computing algorithms forecast the anger expression scales with an acceptable accuracy. However, the accuracy of the DT algorithm is better than the other algorithms.
First GIC Estimates for the Mexican Power Grid
Authors: Caraballo R. , González‐Esparza J. A. , Sergeeva M. , Pacheco C. R.
Published: December 2019

This contribution addresses the first assessment of the impact of geomagnetically induced currents (GIC) on the 400‐kV power grid of Mexico. As an initial approach, we modeled GIC using a uniform conductivity for the entire Mexican territory and spatially uniform geomagnetic disturbance. Power grid data were provided by the electric operator of Mexico; the geophysical data were inferred from the main features of Mexican geology. We calculate the power grid response during four geomagnetic storms from Solar Cycles 23 and 24 (i.e., 15 July 2000, 20 October 2003, 17 March 2015, and 7 September 2017), as well as during an extreme scenario (a Carrington‐like event). The results show that the Mexican power grid can be affected by three‐phase GIC ranging from 20 to 75 A during geomagnetic disturbances. According to the model, sites located in coastal areas or close to the edges of the network can experience large GIC during time intervals between 3 and 10 hr, depending on the intensity of the geomagnetic disturbance. It is an interesting result that these sites are of the major economic and strategic significance for the country. In the case of a Carrington‐like event, the power grid could be affected by GIC ranging between 25 and 150 A under a uniform 1 V/km EW geoelectric field. Such an event might produce significant distortions in the grid hardware (i.e., transformers and static VAR compensators), potentially leading to widespread damage.
Achromatic Non-Interferometric Single Grating Neutron Dark-Field Imaging
Authors: Strobl M. , Valsecchi J. , Harti R. P. , Trtik P. , Kaestner A. , Gruenzweig C. , Polatidis E. , Capek J.
Published: December 2019

We demonstrate a simple single grating beam modulation technique, which enables the use of a highly intense neutron beam for differential phase and dark-field contrast imaging and thus spatially resolved structural correlation measurements in full analogy to interferometric methods. In contrast to these interferometric approaches our method is intrinsically achromatic and provides unprecedented flexibility in the choice of experimental parameters. In particular the method enables straight forward application of quantitative dark-field contrast imaging in time-of-flight mode at pulsed neutron sources. Utilizing merely a macroscopic absorption mask unparalleled length scales become accessible. We present results of quantitative dark-field contrast imaging combining microstructural small angle scattering analyses with real space imaging for a variety of materials.
Elevated CO2 and food ration affect growth but not the size-based hierarchy of a reef fish
Authors: McMahon Shannon J. , Munday Philip L. , Wong Marian Y. L. , Donelson Jennifer M.
Published: December 2019

Under projected levels of ocean acidification, shifts in energetic demands and food availability could interact to effect the growth and development of marine organisms. Changes to individual growth rates could then flow on to influence emergent properties of social groups, particularly in species that form size-based hierarchies. To test the potential interactive effects of (1) food availability, (2) elevated CO2 during juvenile development, and (3) parental experience of elevated CO2 on the growth, condition and size-based hierarchy of juvenile fish, we reared orange clownfish (Amphiprion percula) for 50 days post-hatching in a fully orthogonal design. Development in elevated CO2 reduced standard length and weight of juveniles, by 9% and 11% respectively, compared to ambient. Development under low food availability reduced length and weight of juveniles by 7% and 15% respectively, compared to high food. Parental exposure to elevated CO2 restored the length of juveniles to that of controls, but it did not restore weight, resulting in juveniles from elevated CO2 parents exhibiting 33% lower body condition when reared in elevated CO2. The body size ratios (relative size of a fish from the rank above) within juvenile groups were not affected by any treatment, suggesting relative robustness of group-level structure despite alterations in individual size and condition. This study demonstrates that both food availability and elevated CO2 can influence the physical attributes of juvenile reef fish, but these changes may not disrupt the emergent group structure of this social species, at least amongst juveniles.
A compact LED-based projection microstereolithography for producing 3D microstructures
Authors: Behroodi Ebrahim , Latifi Hamid , Najafi Farhood
Published: December 2019

Projection microstereolithography (PµSL) is a promising additive manufacturing technique due to its low cost, accuracy, speed, and also the diversity of the materials that it can use. Recently it has shown great potentials in various applications such as microfluidics, tissue engineering, micro-optics, biomedical microdevices, and so on. However, studies on PµSL are still ongoing in terms of the quality and accuracy of the construction process, which particularly affect the fabrication of complex 3D microstructures and make it attractive enough to be considered for commercial applications. In this paper, a compact LED-based PµSL 3D printer for the fabrication of 3D microstructures was developed, and the effective parameters that influence the quality of construction were thoroughly investigated and optimized. Accordingly, a customized optical system, including illumination optics and projection optics, was designed using optical engineering principles. This custom 3D printer was proposed for the PµSL process, which besides improving the quality of construction, led to the reduction of the size of the device, its cost-effectiveness, and the repeatability of its performance. To demonstrate the performance of the fabricated device, a variety of complex 3D microstructures such as porous, hollow, helical, and self-support microstructures were constructed. In addition, the repeatability of the device was assessed by fabricating microstructure arrays. The device performance showed that the lateral accuracy of printing was better than 5 μm, and the smallest thickness of the printed layer was 1 μm. Moreover, the maximum printable size of the device was 6.4 mm × 4 mm × 40 mm.
SMO-M2 mutation does not support cell-autonomous Hedgehog activity in cerebellar granule cell precursors
Authors: Petroni Marialaura , Sahùn Roncero Maria , Ramponi Valentina , Fabretti Francesca , Nicolis Di Robilant Vittoria , Moretti Marta , Alfano Vincenzo , Corsi Alessandro , De Panfilis Simone , Giubettini Maria , Di Giulio Stefano , Capalbo Carlo , Belardinilli Francesca , Coppa Anna , Sardina Francesca , Colicchia Valeria , Pedretti Flaminia , Infante Paola , Cardinali Beatrice , Tessitore Alessandra , Canettieri Gianluca , De Smaele Enrico , Giannini Giuseppe
Published: December 2019

Growth and patterning of the cerebellum is compromised if granule cell precursors do not properly expand and migrate. During embryonic and postnatal cerebellar development, the Hedgehog pathway tightly regulates granule cell progenitors to coordinate appropriate foliation and lobule formation. Indeed, granule cells impairment or defects in the Hedgehog signaling are associated with developmental, neurodegenerative and neoplastic disorders. So far, scant and inefficient cellular models have been available to study granule cell progenitors, in vitro. Here, we validated a new culture method to grow postnatal granule cell progenitors as hedgehog-dependent neurospheres with prolonged self-renewal and ability to differentiate into granule cells, under appropriate conditions. Taking advantage of this cellular model, we provide evidence that Ptch1-KO, but not the SMO-M2 mutation, supports constitutive and cell-autonomous activity of the hedgehog pathway.
Modulation of Wnt/β-catenin signaling promotes blood-brain barrier phenotype in cultured brain endothelial cells
Authors: Laksitorini Marlyn D. , Yathindranath Vinith , Xiong Wei , Hombach-Klonisch Sabine , Miller Donald W.
Published: December 2019

Wnt/β-catenin signaling is important for blood-brain barrier (BBB) development and is implicated in BBB breakdown under various pathophysiological conditions. In the present study, a comprehensive characterization of the relevant genes, transport and permeability processes influenced by both the autocrine and external activation of Wnt signaling in human brain endothelial cells was examined using hCMEC/D3 culture model. The hCMEC/D3 expressed a full complement of Wnt ligands and receptors. Preventing Wnt ligand release from hCMEC/D3 produced minimal changes in brain endothelial function, while inhibition of intrinsic/autocrine Wnt/β-catenin activity through blocking β-catenin binding to Wnt transcription factor caused more modest changes. In contrast, activation of Wnt signaling using exogenous Wnt ligand (Wnt3a) or LiCl (GSK3 inhibitor) improved the BBB phenotypes of the hCMEC/D3 culture model, resulting in reduced paracellular permeability, and increased P-glycoprotein (P-gp) and breast cancer resistance associated protein (BCRP) efflux transporter activity. Further, Wnt3a reduced plasmalemma vesicle associated protein (PLVAP) and vesicular transport activity in hCMEC/D3. Our data suggest that this in vitro model of the BBB has a more robust response to exogenous activation of Wnt/β-catenin signaling compared to autocrine activation, suggesting that BBB regulation may be more dependent on external activation of Wnt signaling within the brain microvasculature.
The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model
Authors: Li Zhide , Zhang Chuanshan , Li Liang , Bi Xiaojuan , Li Liang , Yang Shuting , Zhang Ning , Wang Hui , Yang Ning , Abulizi Abuduaini , Aini Abudusalamu , Lin Renyong , Vuitton Dominique A. , Wen Hao
Published: December 2019

The local immune mechanisms responsible for the establishment and development ofEchinococcus granulosus sensu strictoinfection in the liver, have been little explored. We developed a suitable experimental model that mimics naturally infected livers using portal injection of protoscoleces. Opposite toEchinococcus multilocularisinfection which is dose-dependent, fully mature hydatid cysts can be established in the liver whatever the injection dose; although most of the infection sites were seen at the establishment phase as inflammatory granulomas associated with fibrosis, they never matured into cysts. At the establishment phase, a strong immune response was composed of T and B cells, with T1-type, T2-type cells and cytokines and IL-10-secreting CD8+T cells in the liver. At the established phase, results suggested a local production of antibodies by B cells, and an involvement of NK and NKT cells. Infection outcome and local immune response in the liver, were different in the mouse models ofEchinococcus granulosus sensu strictoandEchinococcus multilocularisrespectively; however, only early specificities at the microenvironment level might explain the major differences found between the lesions induced by the two species. Our quantitative experimental model appears fully appropriate to further study this microenvironment and its relationship with each cestode species.
Gender-specific effects of transthyretin on neural stem cell fate in the subventricular zone of the adult mouse
Authors: Vancamp Pieter , Gothié Jean-David , Luongo Cristina , Sébillot Anthony , Le Blay Karine , Butruille Lucile , Pagnin Maurice , Richardson Samantha J. , Demeneix Barbara A. , Remaud Sylvie
Published: December 2019

Choroid plexus epithelial cells produce and secrete transthyretin (TTR). TTR binds and distributes thyroid hormone (TH) to brain cells via the cerebrospinal fluid. The adult murine subventricular zone (SVZ) is in close proximity to the choroid plexus. In the SVZ, TH determines neural stem cell (NSC) fate towards a neuronal or a glial cell. We investigated whether the loss of TTR also disrupted NSC fate choice. Our results show a decreased neurogenic versus oligodendrogenic balance in the lateroventral SVZ of Ttr knockout mice. This balance was also decreased in the dorsal SVZ, but only in Ttr knockout male mice, concomitant with an increased oligodendrocyte precursor density in the corpus callosum. Quantitative RTqPCR analysis following FACS-dissected SVZs, or marked-coupled microbeads sorting of in vitro neurospheres, showed elevated Ttr mRNA levels in neuronal cells, as compared to uncommitted precursor and glial cells. However, TTR protein was undetectable in vivo using immunostaining, and this despite the presence of Ttr mRNA-expressing SVZ cells. Altogether, our data demonstrate that TTR is an important factor in SVZ neuro- and oligodendrogenesis. They also reveal important gender-specific differences and spatial heterogeneity, providing new avenues for stimulating endogenous repair in neurodegenerative diseases.
Ketogenic diet induces skeletal muscle atrophy via reducing muscle protein synthesis and possibly activating proteolysis in mice
Authors: Nakao Reiko , Abe Tomoki , Yamamoto Saori , Oishi Katsutaka
Published: December 2019

Ketogenic diets (KD) that are very high in fat and low in carbohydrates are thought to simulate the metabolic effects of starvation. We fed mice with a KD for seven days to assess the underlying mechanisms of muscle wasting induced by chronic starvation. This diet decreased the weight of the gastrocnemius (Ga), tibialis anterior (TA) and soleus (Sol) muscles by 23%, 11% and 16%, respectively. The size of Ga, TA, Sol muscle fibers and the grip strength of four limbs also significantly declined by 20%, 28%, 16% and 22%, respectively. The muscle atrophy-related genes Mafbx, Murf1, Foxo3, Lc3b and Klf15 were upregulated in the skeletal muscles of mice fed with the KD. In accordance with the reduced expression of anabolic genes such as Igf1, surface sensing of translation (SUnSET) analyses of fast-twitch Ga, TA and Sol muscles revealed that the KD suppressed muscle protein synthesis. The mRNA expression of oxidative stress-responsive genes such as Sod1 was significantly increased in all muscles examined. In addition to hypercorticosteronemia, hypoinsulinemia and reduced IGF-1, oxidative stress might also be involved in KD-induced muscle atrophy. Feeding mice with a KD is a novel experimental animal model of muscle-wasting induced by chronic starvation.
Disordered region of cereblon is required for efficient degradation by proteolysis-targeting chimera
Authors: Kim Kidae , Lee Dong Ho , Park Sungryul , Jo Seung-Hyun , Ku Bonsu , Park Sung Goo , Park Byoung Chul , Jeon Yeong Uk , Ahn Sunjoo , Kang Chung Hyo , Hwang Daehee , Chae Sehyun , Ha Jae Du , Kim Sunhong , Hwang Jong Yeon , Kim Jeong-Hoon
Published: December 2019

Proteolysis targeting chimeras (PROTACs) are an emerging strategy for promoting targeted protein degradation by inducing the proximity between targeted proteins and E3 ubiquitin ligases. Although successful degradation of numerous proteins by PROTACs has been demonstrated, the elements that determine the degradability of PROTAC-targeted proteins have not yet been explored. In this study, we developed von Hippel-Lindau-Cereblon (VHL-CRBN) heterodimerizing PROTACs that induce the degradation of CRBN, but not VHL. A quantitative proteomic analysis further revealed that VHL-CRBN heterodimerizing PROTACs induced the degradation of CRBN, but not the well-known immunomodulatory drug (IMiD) neo-substrates, IKAROS family zinc finger 1 (IKZF1) and −3 (IZKF3). Moreover, truncation of disordered regions of CRBN and the androgen receptor (AR) attenuated their PROTAC-induced degradation, and attachment of the disordered region to stable CRBN or AR facilitated PROTAC-induced degradation. Thus, these results suggest that the intrinsically disordered region of targeted proteins is essential for efficient proteolysis, providing a novel criterion for choosing degradable protein targets.
Capacitive and Sensing Responses of Biomass Derived Silver Decorated Graphene
Authors: Bhujel Rabina , Rai Sadhna , Baruah Khanindram , Deka Utpal , Biswas Joydeep , Swain Bibhu P.
Published: December 2019

A new, easy and green method is utilized for producing silver decorated graphene for its application in sensors and supercapacitors. The biomass-derived silver decorated graphene (AgGr) samples are prepared using an APCVD reactor with varying the process temperature from 600 to 800 °C. The as-synthesized AgGr samples were then characterized by AFM, SEM, Raman spectroscopy, FTIR spectroscopy, XRD, cyclic voltammetry and impedance spectroscopy. The interlayer spacing and ID/IG ratio of the AgGr samples varied from 3.6 to 3.7 Å and 0.87 to 1.52, respectively, as the process temperature was raised from 600 to 800 °C. The SEM image shows the distribution of the flower-like structure of Ag flakes in the graphene sheet for the AgGr-800 sample. Also, the greater number of active sites on the surface of AgGr-800 and the presence of a higher number of defects makes it least useful for p-nitrophenol sensing due to the excess opening of the CV curve but has a maximum capacitance of 93.5 Fg−1 in 1 M H2SO4. AgGr-600 showed extremely good sensing of p-nitrophenol than the other AgGr samples. Therefore this novel technique can be utilized for the large scale manufacture of various metal decorated graphene samples for their application in different fields.
MicroRNA profiles of dry secretions through the first three weeks of the dry period from Holstein cows
Authors: Putz Ellie J. , Putz Austin M. , Jeon Hyeongseon , Lippolis John D. , Ma Hao , Reinhardt Timothy A. , Casas Eduardo
Published: December 2019

In dairy cows, the period from the end of lactation through the dry period and into the transition period, requires vast physiological and immunological changes critical to mammary health. The dry period is important to the success of the next lactation and intramammary infections during the dry period will adversely alter mammary function, health and milk production for the subsequent lactation. MicroRNAs (miRNAs) are small non-coding RNAs that can post transcriptionally regulate gene expression. We sought to characterize the miRNA profile in dry secretions from the last day of lactation to 3, 10, and 21 days post dry-off. We identified 816 known and 80 novel miRNAs. We found 46 miRNAs whose expression significantly changed (q-value < 0.05) over the first three weeks of dry-off. Additionally, we examined the slopes of random regression models of log transformed normalized counts and cross analyzed the 46 significantly upregulated and downregulated miRNAs. These miRNAs were found to be associated with important components of pregnancy, lactation, as well as inflammation and disease. Detailing the miRNA profile of dry secretions through the dry-off period provides insight into the biology at work, possible means of regulation, components of resistance and/or susceptibility, and outlets for targeted therapy development.
Data-driven acceleration of photonic simulations
Authors: Trivedi Rahul , Su Logan , Lu Jesse , Schubert Martin F. , Vuckovic Jelena
Published: December 2019

Designing modern photonic devices often involves traversing a large parameter space via an optimization procedure, gradient based or otherwise, and typically results in the designer performing electromagnetic simulations of a large number of correlated devices. In this paper, we investigate the possibility of accelerating electromagnetic simulations using the data collected from such correlated simulations. In particular, we present an approach to accelerate the Generalized Minimal Residual (GMRES) algorithm for the solution of frequency-domain Maxwell’s equations using two machine learning models (principal component analysis and a convolutional neural network). These data-driven models are trained to predict a subspace within which the solution of the frequency-domain Maxwell’s equations approximately lies. This subspace is then used for augmenting the Krylov subspace generated during the GMRES iterations, thus effectively reducing the size of the Krylov subspace and hence the number of iterations needed for solving Maxwell’s equations. By training the proposed models on a dataset of wavelength-splitting gratings, we show an order of magnitude reduction (~10–50) in the number of GMRES iterations required for solving frequency-domain Maxwell’s equations.
The Rarity of Depth Refugia from Coral Bleaching Heat Stress in the Western and Central Pacific Islands
Authors: Venegas Roberto M. , Oliver Thomas , Liu Gang , Heron Scott F. , Clark S. Jeanette , Pomeroy Noah , Young Charles , Eakin C. Mark , Brainard Russell E.
Published: December 2019

Some researchers have suggested that corals living in deeper reefs may escape heat stress experienced by shallow corals. We evaluated the potential of deep coral reef refugia from bleaching stress by leveraging a long record of satellite-derived sea surface temperature data with a temporal, spatial, and depth precision of in situ temperature records. We calculated an in situ stress metric using a depth bias-adjusted threshold for 457 coral reef sites among 49 islands in the western and central Pacific Ocean over the period 2001–2017. Analysis of 1,453 heating events found no meaningful depth refuge from heat stress down to 38 m, and no significant association between depth and subsurface heat stress. Further, the surface metric underestimated subsurface stress by an average of 39.3%, across all depths. Combining satellite and in situ temperature data can provide bleaching-relevant heat stress results to avoid misrepresentation of heat stress exposure at shallow reefs.
Prevalence of the Hippo Effectors YAP1/TAZ in Tumors of Soft Tissue and Bone
Authors: Isfort Ilka , Elges Sandra , Cyra Magdalene , Berthold Ruth , Renner Marcus , Mechtersheimer Gunhild , Åman Pierre , Larsson Olle , Ratner Nancy , Hafner Susanne , Simmet Thomas , Schliemann Christoph , Rossig Claudia , Dirksen Uta , Grünewald Inga , Wardelmann Eva , Huss Sebastian , Hartmann Wolfgang , Trautmann Marcel
Published: December 2019

Tumors of soft tissue and bone represent a heterogeneous group of neoplasias characterized by a wide variety of genetic aberrations. Albeit knowledge on tumorigenesis in mesenchymal tumors is continuously increasing, specific insights on altered signaling pathways as a basis for molecularly targeted therapeutic strategies are still sparse. The aim of this study was to determine the involvement of YAP1/TAZ-mediated signals in tumors of soft tissue and bone. Expression levels of YAP1 and TAZ were analyzed by immunohistochemistry in a large cohort of 486 tumor specimens, comprising angiosarcomas (AS), Ewing sarcomas, leiomyosarcomas, malignant peripheral nerve sheath tumors (MPNST), solitary fibrous tumors, synovial sarcomas (SySa), well-differentiated/dedifferentiated/pleomorphic and myxoid liposarcomas (MLS). Moderate to strong nuclear staining of YAP1 and TAZ was detected in 53% and 33%, respectively. YAP1 nuclear expression was most prevalent in MPNST, SySa and MLS, whereas nuclear TAZ was predominately detected in AS, MLS and MPNST. In a set of sarcoma cell lines, immunoblotting confirmed nuclear localization of YAP1 and TAZ, corresponding to their transcriptionally active pool. Suppression of YAP1/TAZ-TEAD mediated transcriptional activity significantly impaired sarcoma cell viability in vitro and in vivo. Our findings identify nuclear YAP1 and TAZ positivity as a common feature in subsets of sarcomas of soft tissue and bone and provide evidence of YAP1/TAZ-TEAD signaling as a specific liability to be considered as a new target for therapeutic intervention. Nuclear YAP1/TAZ expression may represent a biomarker suited to identify patients that could benefit from YAP1/TAZ-TEAD directed therapeutic approaches within future clinical trials.
Rapid zero-trans kinetics of Cs+ exchange in human erythrocytes quantified by dissolution hyperpolarized 133Cs+ NMR spectroscopy
Authors: Kuchel Philip W. , Karlsson Magnus , Lerche Mathilde Hauge , Shishmarev Dmitry , Ardenkjaer-Larsen Jan Henrik
Published: December 2019

Transmembrane flux of Cs+ (a K+ congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear magnetic resonance (NMR) spectroscopy with this nuclide in mammalian cells. Four technical developments regularized sample delivery and led to high quality NMR spectra. Cation-free media with the Piezo1 (mechanosensitive cation channel) activator yoda1 maximized the extent of membrane transport. First-order rate constants describing the fluxes were estimated using a combination of statistical methods in Mathematica, including the Markov chain Monte Carlo (MCMC) algorithm. Fluxes were in the range 4–70 μmol Cs+ (L RBC)−1 s−1; these are smaller than for urea, but comparable to glucose. Methodology and analytical procedures developed will be applicable to transmembrane cation transport studies in the presence of additional Piezo1 effectors, to other cellular systems, and potentially in vivo.
Quantitative analysis of the direct piezoelectric response of bismuth ferrite films by scanning probe microscopy
Authors: Kariya Kento , Yoshimura Takeshi , Ujimoto Katsuya , Fujimura Norifumi
Published: December 2019

Polarisation domain structure is a microstructure specific to ferroelectrics and plays a role in their various fascinating characteristics. The piezoelectric properties of ferroelectrics are influenced by the domain wall contribution. This study provides a direct observation of the contribution of domain walls to the direct piezoelectric response of bismuth ferrite (BiFeO3) films, which have been widely studied as lead-free piezoelectrics. To achieve this purpose, we developed a scanning probe microscopy-based measurement technique, termed direct piezoelectric response microscopy (DPRM), to observe the domain structure of BiFeO3 films via the direct piezoelectric response. Quantitative analysis of the direct piezoelectric response obtained by DPRM, detailed analysis of the domain structure by conventional piezoelectric force microscopy, and microscopic characterisation of the direct piezoelectric properties of BiFeO3 films with different domain structures revealed that their direct piezoelectric response is enhanced by the walls between the domains of spontaneous polarisation in the same out-of-plane direction.
Targeting prenylation inhibition through the mevalonate pathway
Authors: Manaswiyoungkul Pimyupa , de Araujo Elvin D. , Gunning Patrick T.
Published: December 2019

Recent progress on mevalonate pathway inhibitor development.
Optical signatures of single ion tracks in ZnO
Authors: Vásquez G. C. , Johansen K. M. , Galeckas A. , Vines L. , Svensson B. G.
Published: December 2019

This paper shows a methodology to identify optical signatures from single ion tracks as an approach to study locally the defects created during ion implantation.
A dinuclear cobalt cluster as electrocatalyst for oxygen reduction reaction
Authors: Li Yun-Wu , Zhang Wen-Jie , Li Chun-Xia , Gu Lin , Du Hong-Mei , Ma Hui-Yan , Wang Su-Na , Zhao Jin-Sheng
Published: December 2019

A Pt-free dinuclear {CoII2} cluster was selected to research its ORR catalytic activities. The {CoII2} possesses defined crystal structure and displays a nice ORR electrocatalytic performance by a nearly 4-electrons reduction pathway.
Synthesis and properties of porous polymers synthesized by Michael addition reactions of multi-functional acrylate, diamine, and dithiol compounds
Authors: Naga Naofumi , Fujioka Shun , Inose Daisuke , Ahmed Kumkum , Nageh Hassan , Nakano Tamaki
Published: December 2019

Porous polymers have been synthesized by Michael addition reactions of multi-functional acrylate and diamine or dithiol compounds.
Preparation of tough, thermally stable, and water-resistant double-network ion gels consisting of silica nanoparticles/poly(ionic liquid)s through photopolymerisation of an ionic monomer and subsequent solvent removal
Authors: Watanabe Takaichi , Takahashi Ruri , Ono Tsutomu
Published: December 2019

A tough, thermally stable, and water-resistant silica nanoparticles/poly(ionic liquid) double-network ion gel was developed.
Effects of Rate Manipulation on Intelligibility in Children With Cerebral Palsy
Authors: Sakash Ashley , Mahr Tristan J. , Natzke Phoebe E. M. , Hustad Katherine C.
Published: December 2019

Purpose

We evaluated the effects of a speech supplementation strategy to reduce rate and improve intelligibility in children with cerebral palsy.

Method

Twenty-five children with cerebral palsy (
M
age
= 12.08 years) completed a structured speaking task in 2 speech conditions: habitual speech and slow speech. Fifteen children had mild intelligibility deficits; 10 had moderate–severe intelligibility deficits. In each condition, children repeated utterances of 2–7 words in length. In the habitual speech condition, children used their natural and unaltered speaking rate. In the slow speech condition, children were cued to insert pauses between words. Intelligibility ratings were obtained from orthographic transcriptions by unfamiliar adult listeners (
n
= 100). Speech rate, in words per minute, was measured for each utterance.

Results

All children, regardless of severity group, were able to reduce their rate of speech when implementing the slow speech strategy. Only children in the moderate–severe group showed an improvement in intelligibility when implementing the slow speech strategy. Although there was considerable individual variability, there was a greater improvement in intelligibility for longer utterances compared to shorter ones.

Conclusion

A slow speech strategy may be beneficial for children with moderate–severe intelligibility deficits who speak in longer utterances. Future studies should seek to further examine the clinical feasibility of slow speech for children with reduced intelligibility.