bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of comorbid constipation on the survival of patients with heart failure: a multicenter, prospective cohort study conducted in Japan
Authors: Ishida Tomoaki , Kawada Kei , Jobu Kohei , Hamada Tomoyuki , Kubo Toru , Okazaki Moemi , Kawai Kazuya , Nakaoka Yoko , Yabe Toshikazu , Furuno Takashi , Yamada Eisuke , Kitaoka Hiroaki , Hamada Yukihiro
Published: January 2025

Background

Constipation frequently affects heart failure patients because of medication side effects and physiological effects of the condition. Although recent speculation suggests that comorbid constipation may affect cardiovascular disease onset and survival rates, this relationship remains unclear. We examined the effect of comorbid constipation on the survival of patients with heart failure.

Methods

We conducted a multicenter prospective cohort study (the Kochi YOSACOI study) of patients hospitalized for acute decompensated heart failure. The influence of comorbid constipation on survival was evaluated using Cox regression analysis with 2-year survival as the index. Patients were divided into two groups based on the presence of comorbid constipation. The patient background was adjusted using propensity score matching, and the evaluation included assessing the 2-year survival and cardiovascular mortality occurrence using the log-rank test.

Results

Among 1,061 patients hospitalized for acute decompensated heart failure, 715 with complete data (124 with comorbid constipation and 591 without) were analyzed. Comorbid constipation was identified as a risk factor for poorer survival in the Cox regression model (hazard ratio: 1.90, 95% confidence interval: 1.3–2.8, P < 0.001). Propensity score matching included 104 patients in each group. Survival analysis using the log-rank test indicated worse survival (P = 0.023) and higher cardiovascular mortality (P = 0.043) in the comorbid constipation group.

Conclusion

Constipation can negatively affect the survival of patients with heart failure. Although the causal link between constipation and decreased survival remains unclear, identifying comorbid constipation is essential for identifying heart failure patients at a higher risk of poor outcomes.
Prognostic implication of right ventricular-pulmonary artery coupling in valvular heart disease
Authors: Wu Zhenni , Xie Mingxing , Zhang Li , He Qing , Gao Lang , Ji Mengmeng , Lin Yixia , Li Yuman
Published: January 2025

Valvular heart disease (VHD) leading to inadequate hemodynamic circulation is a major cause of cardiovascular morbidity and mortality worldwide. Right ventricular-pulmonary artery (RV–PA) coupling integrates the ability of RV contractility to adapt to increased pulmonary arterial afterload. If the right ventricle cannot adapt to the elevated afterload by increasing its contractile function, RV-PA uncoupling occurs. RV-PA uncoupling has been shown to be associated with poor outcomes in VHD. This review summarizes the prognostic significance of RV-PA coupling in patients with VHD.
Reputational concerns moderate the relationship between lying and depression
Authors: Oguni Ryuji , Taguchi Keiya
Published: January 2025

This cross-sectional study examined whether reputational concerns moderated the relationship between lying and depression in adolescence. We conducted an online survey of 1,022 Japanese high school students between the ages of 15 and 18 (474 males and 548 females). Results showed that the relationship between selfish lying and depression was not moderated by either rejection avoidance or praise seeking. In contrast, the relationship between prosocial lying and depression was moderated by both rejection avoidance and praise seeking. Specifically, when rejection avoidance and praise seeking were high and when rejection avoidance was high and praise seeking was low, those with higher tendencies toward prosocial lying exhibited higher levels of depression. When rejection avoidance was low and praise seeking was high, those with higher tendencies toward prosocial lying had lower levels of depression. Our findings indicate that reputational concerns complexly moderate the relationship between lying and depression in adolescence.
Career intervention program to promote academic adjustment and success in higher education
Authors: Sampaio Célia , de Carvalho Catarina Luzia , Taveira Maria do Céu , Silva Ana Daniela
Published: January 2025

Introduction

The transition to higher education can be challenging for many students, requiring developing skills and strategies to help them overcome various challenges. Adjustment to university is a multifaceted process involving several dimensions. Given the difficulty that a significant percentage of students face in this process, the lack of adaptation to higher education has been widely studied, as it is often associated with academic failure and dropping out of school. To mitigate these risks, career interventions have emerged as an essential support, helping students to develop personal resources such as resilience, time management, and decision-making capacity. The aim here is to present a career intervention program focused on promoting the adjustment and academic success of first-year undergraduate or integrated master’s students and evaluating the participants’ feedback on this intervention.

Methodology

This intervention program aimed to provide students with an opportunity to reflect on their adjustment process to the University, identifying possible areas of vulnerability, providing personalized feedback, and proposing modules of learning and personal and academic development that would favor the adjustment and academic success process. Additionally, it aimed to organize, systematize, and make known the support structures existing at the University to which students can resort in possible situations of psychosocial vulnerability and support for academic and career development. This intervention was based on and used a computer platform developed within the framework of the SUnStar project. The intervention took place over 6 weeks using the Teams platform and consisted of three modules, with module two divided into different sessions and topics. Seventy-four university students participated in the intervention, 56 (75.7%) of whom were female. A questionnaire with four Likert-type questions was designed to evaluate the sessions by the students.

Results

The session “Pursuing my UMinho Career: Institutional Support Structures” consistently received the highest ratings across multiple evaluation criteria. In contrast, the “Social Networks and Cooperation” session received the lowest, although all sessions were rated above the average response point. The results are discussed regarding their relevance in an academic context and their implications for future interventions with this target group.
Impact of endometrial thickness and its combined effect with maternal age on singleton adverse neonatal outcomes in frozen–thawed embryo transfer cycles
Authors: Wang Jie , Gao Lina , Huang Qiaoyun , Jiang Weihua , Chen Linjun , Wang Shanshan
Published: January 2025

Background

Thin endometrial thickness (EMT) and advanced age are both common risk factors for adverse neonatal outcomes (ANOs). However, studies evaluating the impact of EMT and combined effect of EMT and age on ANOs remain scarce with conflicts.

Method

A retrospective cohort study was conducted on 7,715 singleton deliveries from frozen embryo transfer (FET) cycles between 2017 and 2021. The participants were categorized into four groups based on EMT quartiles (≤8.5, 8.6–9.5, 9.6–10.5, and >10.5 mm). The primary outcome was preterm birth (PTB), and the secondary outcome included early PTB (EPTB), small/large for gestational age (SGA/LGA), and low birth weight (LBW).

Results

Baseline characteristics were unevenly distributed across EMT groups. After adjusting for the confounders, EMT was found to be associated with the incidence of PTB (aOR 0.922, 95% CI 0.874–0.973, p = 0.003), EPTB (aOR 0.795, 95% CI 0.663–0.954, p = 0.014), LBW (aOR 0.886, 95% CI 0.796–0.986, p = 0.027), and LGA (aOR 1.038, 95% CI 1.004–1.074, p = 0.030). Furthermore, the rates of LBW in the group of EMT at 9.6–10.5 mm (aOR 0.551, 95% CI 0.339–0.895, p = 0.016) and >10.5 mm (aOR 0.536, 95% CI 0.332–0.865, p = 0.011) were lower compared to those with EMT ≤8.5 mm. Among women aged over 35, EMT of 9.6–10.5 mm was associated with a significantly lower incidence of LBW compared to thinner EMT, without increasing the risk of ANOs related to thicker EMT.

Conclusions

Our study demonstrated the independent nonlinear impact of EMT on PTB, EPTB, LGA, and LBW. It provided new insights into the combined effects of EMT and age in FET cycles and offered valuable references for the clinical management and treatment strategies aimed at EMT.
The impact of ecological civilization construction on the resilience of green technology innovation: evidence based on double dual machine learning
Authors: Li Cunlin , Zhang Shiyu , Yang Baojun
Published: January 2025

Can the accelerated development of ecological civilization promote the sustainable development of green innovation?This paper investigates the effects of ecological civilization demonstration zones (ECDZs) on green innovation resilience. Based on a sample of 237 prefecture-level cities across 31 provinces in China from 2011 to 2021, our double dual machine learning and spatial difference-in-differences model indicates that ECDZs significantly enhance urban green innovation resilience. Our findings also reveal a spatial spillover effect of ECDZ—the development of ECDZs in one city significantly improves the resilience of green innovation in neighboring cities. The spatial spillover effect reaches its maximum in the fifth year. Our analysis of the underlying mechanisms suggests that ECDZs promote urban green innovation resilience through the advancement of digitalization, green consciousness, and new quality productivity. We also conduct an analysis of heterogeneity based on geographical locations and levels of policy support, and the results show that the impact of ECDZs on urban green innovation resilience is mainly observed in western, inland, and strongly policy-supported regions. The findings of this study provide crucial insights and valuable guidance for developing national environmental conservation policies and programs.
Integrating single-cell RNA-seq and bulk RNA-seq to explore prognostic value and immune landscapes of methionine metabolism-related signature in breast cancer
Authors: Gao Yanxian , Feng Ziyu , Zhao Hailong , Liu Xinghai , Zhu Muyu , Yu Xiafei , Liu Xiaoan , Wu Xian , Tao Jing
Published: January 2025

Background

Neoadjuvant, endocrine, and targeted therapies have significantly improved the prognosis of breast cancer (BC). However, due to the high heterogeneity of cancer, some patients cannot benefit from existing treatments. Increasing evidence suggests that amino acids and their metabolites can alter the tumor malignant behavior through reshaping tumor microenvironment and regulation of immune cell function. Breast cancer cell lines have been identified as methionine-dependent, and methionine restriction has been proposed as a potential cancer treatment strategy.

Methods

We integrated transcriptomic and single-cell RNA sequencing (ScRNA-seq) analyses based on The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Then we applied weighted gene co-expression network analysis (WGCNA) and Cox regression to evaluate methionine metabolism-related genes (MRGs) in BC, constructing and validating a prognostic model for BC patients. Immune landscapes and immunotherapy were further explored. Finally, in vitro experiments were conducted to assess the expression and function of key genes APOC1.

Results

In this study, we established and validated a prognostic signature based on eight methionine-related genes to predict overall survival (OS) in BC patients. Patients were further stratified into high-risk and low-risk groups according to prognostic risk score. Further analysis revealed significant differences between two groups in terms of pathway alterations, immune microenvironment characteristics, and immune checkpoint expression. Our study shed light on the relationship between methionine metabolism and immune infiltration in BC. APOC1, a key gene in the prognostic signature, was found to be upregulated in BC and closely associated with immune cell infiltration. Notably, APOC1 was primarily expressed in macrophages. Subsequent in vitro experiments demonstrated that silencing APOC1 reduced the generation of tumor-associated macrophages (TAMs) with an M2 phenotype while significantly decreasing the proliferation, invasion, and migration of MDA-MB-231 and MDA-MB-468 breast cancer cell lines.

Conclusion

We established a prognostic risk score consisting of genes associated with methionine metabolism, which helps predict prognosis and response to treatment in BC. The function of APOC1 in regulating macrophage polarization was explored.
Upregulation of interferon-γ response genes in monocytes and T cells identified by single-cell transcriptomics in patients with anti-citrullinated peptide antibody-positive early rheumatoid arthritis
Authors: Hong Bong-Ki , You Sungyong , Kim Jung Gon , Kim Minhyung , Lee Naeun , Lee Kijun , Baek In-Pyo , Ju Ji Hyeon , Kim Wan-Uk , Kim Ho-Youn
Published: January 2025

Introduction

Our aim was to investigate the insufficiently understood differences in the immune system between anti-citrullinated peptide antibody (ACPA)-positive (ACPA+) and ACPA-negative (ACPA-) early rheumatoid arthritis (eRA) patients.

Methods

We performed multiple cytokine assays using sera from drug-naïve ACPA+ and ACPA- eRA patients. Additionally, we conducted single-cell RNA sequencing of CD45+ cells from peripheral blood samples to analyze and compare the distribution and functional characteristics of the cell subsets based on the ACPA status.

Results

Serum concentrations of interferon-γ (IFN-γ) and interleukin (IL)-12 were higher in ACPA+ eRA than in ACPA- eRA. Single-cell transcriptome analysis of 37,318 cells identified 17 distinct cell types and revealed the expansion of IL1B+ proinflammatory monocytes, IL7R+ T cells, and CD8+ CCL4+ T cells in ACPA+ eRA. Furthermore, we observed an enrichment of IFN-γ response genes in nearly all monocytes and T cells of ACPA+ eRA subsets. Heightened interactions between IFN-γ and IFN-γ receptors were observed in ACPA+ eRA, particularly between monocytes and T cells. We examined IFITM2 and IFITM3 as potential key markers in ACPA+ eRA given their pronounced upregulation and association with the IFN response. Specifically, the expression of these genes was elevated in IL1B+ proinflammatory monocytes (likely M1 monocytes), correlating with serum IFN-γ levels.

Discussion

Compared to ACPA- eRA, ACPA+ eRA showed higher serum IFN-γ and IL-12 levels, upregulated IFN-γ response genes, and enhanced IFN-γ-driven monocyte-T cell interactions. These distinct immune features of the peripheral circulation in ACPA+ eRA suggest a role for type 1 helper T cell-related immunity in its pathogenesis.
The characteristics of the tumor immune microenvironment in colorectal cancer with different MSI status and current therapeutic strategies
Authors: Wang Qingzhe , Yu Min , Zhang Shuang
Published: January 2025

Colorectal cancer (CRC) remains a significant cause of cancer-related mortality worldwide. Despite advancements in surgery, chemotherapy, and radiotherapy, the effectiveness of these conventional treatments is limited, particularly in advanced cases. Therefore, transition to novel treatment is urgently needed. Immunotherapy, especially immune checkpoint inhibitors (ICIs), has shown promise in improving outcomes for CRC patients. Notably, patients with deficient mismatch repair (dMMR) or microsatellite instability-high (MSI-H) tumors often benefit from ICIs, while the majority of CRC cases, which exhibit proficient mismatch repair (pMMR) or microsatellite-stable (MSS) status, generally show resistance to this approach. It is assumed that the MSI phenotype cause some changes in the tumor microenvironment (TME), thus triggering antitumor immunity and leading to response to immunotherapy. Understanding these differences in the TME relative to MSI status is essential for developing more effective therapeutic strategies. This review provides an overview of the TME components in CRC and explores current approaches aimed at enhancing ICI efficacy in MSS CRC.
Case report: Overlapping syndrome of MOG-IgG associated optic neuritis and autoimmune encephalitis with co-existence of anti-NMDAR and anti-GABABR antibodies
Authors: Mei Zhujun , Wang Jingguo , Wang Junling , Liu Xiaoni , Deng Bo , Yang Wenbo , Zhang Xiang , Chen Xiangjun , Yu Hai
Published: January 2025

We report a case of optic neuritis (ON) secondary to autoimmune encephalitis (AE) in a patient with concomitant antibodies to N-methyl-D-aspartate receptor (NMDAR), gamma-aminobutyric acid-B receptor (GABABR), and myelin oligodendrocyte glycoprotein (MOG). The patient exhibited a constellation of symptoms, including vision loss, seizures, mental and behavioral disorders, cognitive impairment, and speech abnormalities. At the two-year follow-up, the patient’s symptoms had abated entirely. Overlap syndrome of triple autoimmune antibodies is rare and the coexistence of antibodies to NMDAR, GABABR and MOG has not been reported till now. This case report provides novel experience of diagnosis and treatment in autoimmune overlap syndromes.
Venetoclax in combination with chidamide and azacitidine for the treatment of relapsed/refractory B-cell acute lymphoblastic leukemia with the MLL-AF4 gene: a case report and literature review
Authors: Jin Xuelian , Liu Zhigang , Wu Yu , Ji Jie
Published: January 2025

B-cell acute lymphoblastic leukemia (B-ALL) with the MLL-AF4 fusion gene has a poor prognosis, and the mortality rate exceeds 90%, particularly in cases of extramedullary relapse (EMR). Herein, we present a case of a 46-year-old male patient who developed relapsed B-ALL with MLL-AF4. The patient initially achieved a complete remission (CR) after induction therapy and underwent haploidentical hematopoietic stem cell transplantation. Five months post-transplantation, he developed enlarged lymph nodes and subcutaneous masses. A lymph node biopsy confirmed EMR, without leukemia in the bone marrow or peripheral blood. The patient received the VCA regimen (venetoclax, chidamide, and azacitidine) and was regularly monitored through blood counts, marrow cell morphology analysis, flow cytometry, and computed tomography or positron emission tomography-computed tomography imaging. After the first VCA course, the patient achieved a second CR with only transient myelosuppression. Following two VCA courses, he received chimeric antigen receptor T-cell therapy, which led to complete metabolic remission and improved prognosis. This case underscores the potential of the VCA regimen as a bridging therapy for EMR in B-ALL with MLL-AF4, although further studies are warranted.
From pain to meningitis: bacteria hijack nociceptors to promote meningitis
Authors: Liu Huazhen , Kong Xingxing , Zeng Yuqin , Chen Jinyun , Chen Zhanpeng , Liu Lanlan , Ma Quan , Liu Xuhui , Lu Shuihua
Published: January 2025

Bacterial meningitis is a severe and life-threatening infection of the central nervous system (CNS), primarily caused by Streptococcus pneumoniae and Neisseria meningitidis. This condition carries a high risk of mortality and severe neurological sequelae, such as cognitive impairment and epilepsy. Pain, a central feature of meningitis, results from the activation of nociceptor sensory neurons by inflammatory mediators or bacterial toxins. These nociceptors, abundantly present in the meninges, trigger neuroimmune signaling pathways that influence the host immune response. However, the mechanisms by which bacteria hijack these nociceptors to promote CNS invasion and exacerbate the disease remain poorly understood. This review examines the interactions between bacteria and meningeal nociceptors, focusing on their direct and indirect activation via ion channels, such as transient receptor potential vanilloid-1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1), or through the release of neuropeptides like calcitonin gene-related peptide (CGRP). These interactions suppress immune defenses by inhibiting macrophage activity and neutrophil recruitment, thus facilitating bacterial survival and invasion of the CNS. Understanding this neuroimmune axis may open potential therapeutic targets for treating bacterial meningitis by enhancing host defenses and mitigating pain. Further research using advanced methodologies is essential to clarify the role of nociceptor-mediated immune modulation in this disease.
Advancements in targeted and immunotherapy strategies for glioma: toward precision treatment
Authors: Gong Guangyuan , Jiang Lang , Zhou Jing , Su Yuanchao
Published: January 2025

In recent years, significant breakthroughs have been made in cancer therapy, particularly with the development of molecular targeted therapies and immunotherapies, owing to advances in tumor molecular biology and molecular immunology. High-grade gliomas (HGGs), characterized by their high malignancy, remain challenging to treat despite standard treatment regimens, including surgery, radiotherapy, chemotherapy, and tumor treating fields (TTF). These therapies provide limited efficacy, highlighting the need for novel treatment strategies. Molecular targeted therapies and immunotherapy have emerged as promising avenues for improving treatment outcomes in high-grade gliomas. This review explores the current status and recent advancements in targeted and immunotherapeutic approaches for high-grade gliomas.
Establishment and maturation of gut microbiota in White King pigeon squabs: role of pigeon milk
Authors: Xu Xiaoqin , Wang Zihan , Jian Yi , Zhang Long , Zhou Caiquan , Liu Li , Liu Hui
Published: January 2025

Background

Pigeons are significant economic animals in China; however, research regarding the establishment and influencing factors of gut microbiota in squabs remains limited. Understanding how the gut microbiota develops in pigeons, particularly in relation to pigeon milk, is importance in pigeon production. This study aims to elucidate the establishment characteristics of the gut microbiota in White King pigeon squabs and explore the role of pigeon milk in this process.

Methods

This study employed 16S rRNA sequencing technology to investigate the dynamics of microbial composition in feces and pigeon milk at various growth stages of White King pigeon. Functional prediction analysis was performed to assess the metabolic pathways involved, and correlation analysis was used to explore the relationships between microbial communities in different sample types.

Results

The findings revealed a diverse microbiome present in the meconium of newborn pigeons, with a microbial composition that significantly differed from that of other feces groups. In contrast, the microbial composition of feces (FN) from pigeons aged 7 to 21 days exhibited less variability. At the phylum level, the predominant microbial taxa identified in the feces of FN were Firmicutes, Actinobacteriota, and Proteobacteria. At the genus level, the main dominant bacterial groups included Lactobacillus, Limosilactobacillus, and Turicibacter. Functional prediction analysis indicated that the gut microbiota of pigeons primarily participate in metabolic pathways related to carbohydrates, amino acids, lipids, cofactors, and vitamins. Furthermore, the dominant bacteria found in pigeon milk (MN) were identified as probiotics, including Limosilactobacillus, Ligilactobacillus, Lactobacillus, Bifidobacterium, and Aeriscardovia, which collectively accounted for over 90% of the total abundance. Correlation analysis of the abundance of shared microbes revealed that the association between meconium and feces at the other stages was extremely low. In contrast, the correlation between colostrum and feces at the post-feeding stage were found to be the highest.

Conclusion

This study indicates that prenatal colonization occurs in White King pigeons. Notably, within the first week after birth, the gut microbial composition of young pigeons becomes stable. Furthermore, the colostrum serves as the most significant driver for the establishment of intestinal microbiota in squab post-birth. The findings of this study suggest that microorganisms can be added to artificial pigeon milk based on the predominant microbial composition of colostrum. This approach could facilitate the establishment of gut microbiota in young pigeons, thereby promoting their growth and development and providing production benefits.
Vic9 mycobacteriophage: the first subcluster B2 phage isolated in Russia
Authors: Zaychikova Marina , Malakhova Maja , Bespiatykh Dmitry , Kornienko Maria , Klimina Ksenia , Strokach Aleksandra , Gorodnichev Roman , German Arina , Fursov Mikhail , Bagrov Dmitry , Vnukova Anna , Gracheva Alexandra , Kazyulina Anastasia , Shleeva Margarita , Shitikov Egor
Published: January 2025

Mycobacteriophages are viruses that specifically infect bacteria of the Mycobacterium genus. A substantial collection of mycobacteriophages has been isolated and characterized, offering valuable insights into their diversity and evolution. This collection also holds significant potential for therapeutic applications, particularly as an alternative to antibiotics in combating drug-resistant bacterial strains. In this study, we report the isolation and characterization of a new mycobacteriophage, Vic9, using Mycobacterium smegmatis mc (2)155 as the host strain. Vic9 has been classified within the B2 subcluster of the B cluster. Morphological analysis revealed that Vic9 has a structure typical of siphophages from this subcluster and forms characteristic plaques. The phage adsorbs onto host strain cells within 30 min, and according to one-step growth experiments, its latent period lasts about 90 min, followed by a growth period of 150 min, with an average yield of approximately 68 phage particles per infected cell. In host range experiments, Vic9 efficiently lysed the host strain and also exhibited the ability to lyse M. tuberculosis H37Rv, albeit with a low efficiency of plating (EOP ≈ 2 × 10−5), a typical feature of B2 phages. No lysis was observed in other tested mycobacterial species. The genome of Vic9 comprises 67,543 bp of double-stranded DNA and encodes 89 open reading frames. Our analysis revealed unique features in Vic9, despite its close relationship to other B2 subcluster phages, highlighting its distinct characteristics even among closely related phages. Particularly noteworthy was the discovery of a distinct 435 bp sequence within the gene cluster responsible for queuosine biosynthesis, as well as a recombination event within the structural cassette region (Vic_0033-Vic_0035) among members of the B1, B2, and B3 subclusters. These genetic features are of interest for further research, as they may reveal new mechanisms of phage-bacteria interactions and their potential for developing novel phage therapy methods.
NDUFA11 may be the disulfidptosis-related biomarker of ischemic stroke based on integrated bioinformatics, clinical samples, and experimental analyses
Authors: Li Sijun , Chen Ningyuan , He Junrui , Luo Xibao , Lin Wei
Published: January 2025

Background

Programmed cell death plays an important role in neuronal injury and death after ischemic stroke (IS), leading to cellular glucose deficiency. Glucose deficiency can cause abnormal accumulation of cytotoxic disulfides, resulting in disulfidptosis. Ferroptosis, apoptosis, necroptosis, and autophagy inhibitors cannot inhibit this novel programmed cell death mechanism. Nevertheless, the potential mechanisms of disulfidptosis in IS remain unclear.

Methods

The GSE16561 dataset was used to screen for differentially expressed disulfidptosis-related biomarkers (DE-DRBs). A correlation between the DE-DRBs was detected. The optimal machine-learning (ML) model and predictor molecules were determined. The GSE58294 dataset was used to verify the accuracy of the optimal ML model. The DE-DRB expression was detected in the blood of patients with IS. Based on IS models, experimental analyses were performed to verify DE-DRB expression and the correlation between DE-DRBs.

Results

Leucine-rich pentatricopeptide repeat-containing (LRPPRC) and NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (NDUFA11) were identified as DE-DRBs. The NADH: ubiquinone oxidoreductase core subunit S1 (NDUFS1) interacted with NDUFA11 and LRPPRC. The support vector machine (SVM) model was identified as the optimal ML model. The NDUFA11 expression level in the blood of patients with IS was 20.9% compared to that in normal controls. NDUFA11 expression was downregulated in the in vitro/in vivo models of IS. The number of formed complexes of NDUFS1 and NDUFA11 decreased in the in vitro/in vivo models of IS.

Conclusion

This research suggests that NDUFA11 is a specific DRB for IS and demonstrates alterations in the disulfidptosis-related protein complexes NDUFS1-NDUFA11.
Effects of acupuncture at the Taichong (LIV3) and Hegu (LI4) points on functional connectivity with the retrosplenial cortex in patients with Alzheimer’s disease
Authors: Wang Junkai , Bai Xinyue , Chen Xiaojun , Liu Shui , Sun Mengmeng , Li Kuncheng , Zheng Yunsong , Wang Zhiqun
Published: January 2025

Background

Acupuncture has been demonstrated to have a promising effect on Alzheimer’s disease (AD), but the underlying neural mechanisms remain unclear. The retrosplenial cortex (RSC) is one of the earliest brain regions affected in AD, and changes in its functional connectivity (FC) are reported to underlie disease-associated memory impairment. The aim of this study was to examine the effect of acupuncture on FC with the RSC in patients with AD.

Methods

Demographic data, neuropsychological assessments, and resting-state functional magnetic resonance imaging (fMRI) data were collected from 14 AD patients and 14 normal controls (NCs) matched by age, sex, and educational level at baseline. After the baseline MRI scan, acupuncture stimulation on the Taichong (LIV3) and Hegu (LI4) points was performed for 3 min. Then, another 10 min of fMRI data were acquired after the needle was withdrawn. A dataset that included 100 healthy participants was also included to construct a reliable FC map of the RSC. Two sets of regions of interest (ROIs) in the RSC were selected to assess the sustained effect of acupuncture on FC with the RSC in AD patients and NCs.

Results

Two sets of RSC ROI-based analyses demonstrated robust positive connectivity with the hippocampus (HPC). Furthermore, multiple brain regions, including the bilateral thalamus, bilateral posterior cingulate cortex (PCC), bilateral subcallosal cingulate gyrus (SCG), bilateral orbitofrontal cortex (OFC), and right precuneus, showed decreased FC with the RSC in the AD group and increased FC with the RSC in the NC group after acupuncture compared to that at baseline. Acupuncture also specifically elicited increased FC between the RSC and the HPC as well as between the RSC and the parahippocampal gyrus in AD patients and decreased FC between the RSC and the visual cortices in NCs. Additionally, diminished FC with the RSC was correlated with neuropsychological scale scores in the AD group before acupuncture treatment.

Conclusion

These findings confirm and extend previous studies suggesting that acupuncture at Taichong (LIV3) and Hegu (LI4) can exert bidirectional and benign regulatory effects on RSC connectivity in AD patients.
Characterization of the volatile compounds in tea (Camellia sinensis L.) flowers during blooming
Authors: Guo Xiangyang , Wang Lulu , Huang Xiuting , Zhou Qiying
Published: January 2025

Tea flower, with characteristic flavor formed during blooming, are a significant tea resource. However, studies on the volatile compounds of tea flower and their aroma characteristics during flowering are scarce. In this study, the odor characteristics of tea flower during blooming were comprehensively investigated by GC–MS, PCA, ACI determination and sensory evaluation. The tea flower of unopened buds (TF-S1) contained the highest alcohol amounts, while fully opened tea flowers (TF-S3) had the highest heterocyclic compounds. Half-opened tea flowers (TF-S2) had the most volatile compounds, including high levels of linalool and its oxides, and low levels of (Z)-3-hexen-1-ol. Acetoin and cosmene were first identified in TF-S1 and TF-S2, respectively. The major ACI components differed, with linalool being prominent exhibiting ACI above 27 in all samples. Acetophenone, unique to TF-S2 with ACI of 57.35, contributed to sweet odor. Furthermore, PCA analysis and sensory evaluation revealed distinct aroma characteristics among the samples. Overall, TF-S2 and TF-S3 had higher volatile amounts and better aroma properties with floral, powdery or almond-like odors. These results advance the understanding of aroma properties of tea flower during blooming, and provide a reference for resource utilization and promotion of the application in food or cosmetics industries.
Prognostic significance of programmed cell death 1 expression on CD8+T cells in various cancers: a systematic review and meta-analysis
Authors: Wan Zhiyong , Cui Meng , Yang Jia , Liao Dan , Chen Junliang , Li Fanmin , Xiang Yin , Cui Zhiwei , Yang Yang
Published: January 2025

Background

Increased PD-1 expression on CD8+ T cells is considered as a hallmark for T-cell exhaustion, and is thought to be related to the prognosis of cancer patients. However, discrepant results have made it difficult to apply PD-1+CD8+T cells and tumor prognosis to clinical practice. Therefore, we conducted a meta-analysis to evaluate its prognostic value in human cancers.

Methods

PRISMA reporting guidelines were strictly followed for conducting the current meta-analysis. The PubMed, Web of Science, Embase databases were searched from inception to November 2024. The pooled Hazard Ratio (HR) along with 95% confidence intervals (CIs) of each article were combined for the associations of PD-1+CD8+ T cells with overall survival (OS), progression- free survival (PFS) and disease-free survival(DFS). Subgroup analyses were performed for area, specimen type, cancer type, treatment, detected method and cancer stage.

Results

A total of 20 studies (23 cohorts, 3086 cancer patients) were included in our study. The expression PD-1+CD8+ T cells in cancer patients tended to predict poor overall survival (OS) (HR: 1.379, 95%CI: 1.084-1.753, p= 0.009), and unfavorable disease-free survival(DFS) (HR: 1.468, 95%CI: 0.931-2.316, p=0.099), though it did not reach statistical significance. Begg’s and Egger’s test demonstrated that no obvious publication bias was exist.

Conclusions

High PD-1 expression on CD8+ T cells is associated with worse survival outcomes, which can be potentially used as a prognostic marker of malignant tumor.
Efficacy and safety of hemoporfin photodynamic therapy in treating port-wine stains in Chinese children: a systematic review and meta-analysis
Authors: Xu Jing , Li Hongxin
Published: January 2025

Objective

The purpose of this study is to explore the efficacy and safety of hematoporphyrin monomethyl ether mediated photodynamic therapy (HMME-PDT) in treating children with port-wine stains (PWS).

Method

Literature related to the topic was searched in PubMed, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure, Wanfang, and China Science Technology Journal Database online databases. The quality of the literature was evaluated using the Effective Public Health Practice Project. The I2 statistic was used to evaluate the consistency of the results.

Results

A total of 19 papers were included. Meta-analysis showed that more than half of the children (56.3%) achieved efficacy I (improvement ≥ 60%). 17% of children achieved efficacy II (improvement ≥ 75%). Regardless of whether the outcome variable was efficacy I or efficacy II, the therapeutic efficacy in children with PWS aged 0–3 years was superior to those aged 3–6 and 6–18 years, and children who underwent a treatment course of ≥3 sessions showed better outcomes compared to those who have only 1 or 2 sessions. After treatment with HMME-PDT, better efficacy was seen in the PWS of the face and neck and pink/red PWS. Additionally, almost all children with PWS treated with HMME-PDT developed edema (99.9%), more than half presented purpura (67.6%), some developed crust (30.8%) and hyperpigmentation (15.0%), and a few occurred scar (2.4%) and hypopigmentation (1.4%).

Conclusion

After HMME-PDT treatment, more than half of the pediatric patients showed an improvement of ≥60%, and no serious adverse reaction events occurred. This study demonstrated that HMME-PDT possessed promising therapeutic efficacy in children with PWS, suggesting that HMME-PDT could be considered a recommended treatment strategy for pediatric PWS. However, future development of standardized assessment guidelines and comparative studies are needed to validate the aforementioned conclusions.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/#loginpage, PROSPERO (CRD42024592367).
PbMYB5 transcription factor plays a role in regulating anthocyanin biosynthesis in pear (Pyrus bretschneideri Rehd) skin
Authors: Li Shangyun , Yang Yutao , Zhou Zhiwei , Zhou Xuan , Lei Diya , He Ruiyuan , Zhang Yunting , Zhang Jiliang , Lin Yuanxiu , Wang Yan , Li Mengyao , He Wen , Chen Qing , Luo Ya , Wang Xiaorong , Tang Haoru , Zhang Yong
Published: January 2025

The phenylacetone pathway, which encompasses flavonoids, lignin, and other compounds, is of paramount importance in determining the quality of pear fruit. Nevertheless, the precise regulatory functions of R2R3-MYB transcription factors in the metabolic pathways that regulate pear color changes remain unclear. In this study, we isolated an R2R3-PbMYB5(PbMYB5) transcription factor from ‘Red Zaosu’ pears and demonstrated that it influenced the expression of several genes, including PbCAD1, PbF5H, PbLAR, PbANR, and PbUFGT. The overexpression of PbMYB5 resulted in a notable elevation in anthocyanin concentration within the pear epidermis. Further research has shown that PbMYB5 is able to bind to PbANS and also has interactions with PbbHLH3 and PbbHLH33.We proposed that PbMYB5 forms a complex with PbbHLH3, PbbHLH33, and PbWD40 to activate PbANS and promote anthocyanin accumulation. This study offers new insights into the regulation of various metabolic pathways that impact fruit coloration.
Social technology and rescue of native seeds in the Venezuelan Andes Páramo
Authors: Lugo Montilla Iraima , Águas Carla Ladeira Pimentel
Published: January 2025

This study analyzes the recovery and conservation of potato landraces in the Venezuelan Andes Páramo, specifically in the Mérida region. Smallholder potato farmers in the Venezuelan Andes have been actively creating knowledge, processes, and socio-technical artifacts necessary for the recovery of potato seeds and other types of plant genetic resources critical for food and agriculture. This effort has involved diverse socio-technical dynamics, engaging both human and non-human actors, with the support of universities, research institutions, local and national governments, etc. These initiatives counter historical trends, such as the erosion of complex genetic resource systems, genetic resources, the impacts of agricultural modernization, dependency on oil and imported agricultural technologies, and more recent challenges, such as food shortages. Using traditional techniques such as Tinopós—underground caves for seed storage—and social appropriation of technological processes, including genetic improvement, Andean smallholder farmers have successfully rescued ancient potato varieties while developing new ones. Fieldwork conducted in 2019 included a documentary review, thematic analysis, and a technology inventory. The findings reveal a close relationship between the diversity of Andean root and tuber crops, the unique biophysical and climatic conditions of the Páramo, and the traditional knowledge of peasant communities residing in these territories. The study underscores the importance of border relations and cultural translation between different knowledge systems in recovering and conserving native potato seeds. These efforts represent a locally driven initiative to construct social technologies for food sovereignty and sustainable local development.
Role of the ventral portion of intermediate arcopallium in stability of female Bengalese finch song preferences
Authors: Coulter Austin , Prather Jonathan F.
Published: January 2025

The process of decision making is a complex procedure influenced by both external and internal conditions. Songbirds provide an excellent model to investigate the neural mechanisms of decision making, because females rely on acoustic signals called songs as important stimuli in directing their mate choice. Previous experiments by our group and others have implicated secondary auditory brain sites in female evaluation of song quality, including the caudal portions of the nidopallium (NC) and mesopallium (CM). Recent pathway tracing experiments reveal a convergence of those sites onto a third region, the ventral portion of the intermediate arcopallium (AIV), suggesting that AIV may also play an important role in song evaluation and mate choice. Here we combined behavioral testing with lesion inactivation to investigate the role of AIV in song preference of female Bengalese finches (Lonchura striata domestica). Inactivation of AIV was associated with destabilization of rank ordering of song preferences. These data suggest a model in which the convergence of auditory activity in AIV plays an important role in female perception of song quality and production of courtship behaviors. Together with previous results that also demonstrate a role for the auditory areas that converge onto AIV, these findings extend the experimental tractability of this emerging animal model of sensory perception and decision making.
Theta transcranial alternating current stimulation over the prefrontal cortex enhances theta power and working memory performance
Authors: Debnath Ranjan , Elyamany Osama , Iffland Jona Ruben , Rauh Jonas , Siebert Michael , Andraes Elisa , Leicht Gregor , Mulert Christoph
Published: January 2025

Introduction

Transcranial alternating current stimulation (tACS) is a promising tool for modulating brain oscillations. This study investigated whether 5 Hz tACS could modulate neural oscillations in the prefrontal cortex and how this modulation impacts performance in working memory (WM) tasks.

Method

In two sessions, 28 healthy participants received 5 Hz tACS or sham stimulation over the left dorsolateral prefrontal cortex (DLPFC) while performing tasks with high and low WM loads. Resting-state EEG was recorded before and after stimulations for 5 minutes. EEG power was measured at electrodes surrounding the stimulation site.

Results

The results showed that tACS significantly improved reaction time (RT) compared to sham stimulation. This effect was task-specific, as tACS improved RT for hit responses only in high WM load trials, with no impact on low-load trials. Moreover, tACS significantly increased EEG power at 5 Hz and in the theta band compared to pre-stimulation levels.

Discussion

These findings demonstrate that tACS applied over left DLPFC modulates post-stimulation brain oscillations at the stimulation sites – known as tACS after-effects. Furthermore, the results suggest that 5 Hz tACS enhances response speed by elevating task-related activity in the prefrontal cortex to an optimal level for task performance.

Conclusion

In summary, the findings highlight the potential of tACS as a technique for modulating specific brain oscillations, with implications for research and therapeutic interventions.
Factors influencing childbirth fear among Asian women: a scoping review
Authors: Kalok Aida , Kamisan Atan Ixora , Sharip Shalisah , Safian Nazarudin , Shah Shamsul Azhar
Published: January 2025

Fear of childbirth (FOC) or tokophobia adversely affects women during pregnancy, delivery, and postpartum. Childbirth fear may differ across regions and cultures. We aimed to identify factors influencing the fear of childbirth among the Asian population. A systematic literature search was performed using the PubMed, Scopus, and Web of Science databases in November 2023. Original articles in English with research conducted in Asian countries were included. The independent factors associated with childbirth fear, from the relevant studies were identified and discussed. Forty-six papers met the eligibility criteria but only 26 studies were discussed in this review. The significant factors were categorized into (1) demographics, (2) clinical, (3) healthcare service, (4) childbirth education & information, and (5) COVID-19 pandemic. The prevalence of childbirth fear among Asians ranged between 56.6 and 84.8%. Significant demographic factors included age, education, marital status, economic status, and area of residence. Greater levels of tokophobia were linked to nulliparity, unplanned pregnancy, infertility, miscarriage, and pregnancies at risk. Effective doctor-patient communication and more frequent antenatal visits were shown to alleviate maternal childbirth fear. There was consistent evidence of prenatal childbirth education’s benefit in reducing FOC. The usage of smartphone apps and prolonged exposure to electronic devices were linked to a higher degree of tokophobia. Nulliparas who received too much pregnancy-related information also reported increased childbirth fear. There was a positive correlation between maternal fear of COVID-19 infection and FOC. Keeping updated with COVID-19 information increased the maternal childbirth fear by two-fold. In conclusion, a stable economy and relationship contribute to lesser childbirth fear among Asian women. Poor maternal health and pregnancy complications were positive predictors of FOC. Health practitioners may reduce maternal childbirth through women’s education, clear communication as well as accurate information and guidance to expectant mothers. Further study is required into the content of childbirth fear among Asian women. These research findings hopefully will lead to the development of culturally adapted screening tools and interventions that reduce the burden of FOC among expectant mothers.