bluuebunny/crossref_metadata_2025 huggingface dataset:
The Assessment of the Possibility of Using Ethanol and Ultrasound to Design the Properties of Dried Carrot Tissue
Authors: Dadan Magdalena , Nowacka Malgorzata
Published: January 2021

The purpose of this study was to investigate the effect of pre-treatment in ethyl alcohol for 5, 15, 60 and 180 s with the application of ultrasound on the course of convective drying and properties of carrot tissue directly after the treatment and after the drying process. The treatment in ethanol resulted in loss of mass, increase of dry matter, ethanol conductivity, extractivity of carotenoids with a slight effect on the colour of carrot tissue after the treatment. The utilization of ultrasound during immersion in ethanol contributed to additional increase of conductivity of ethanol, and extractivity of carotenoids. The immersion in ethanol virtually did not affect the drying kinetics, which can be explained by the increase of shrinkage of the tissue in relation to the untreated dried tissue. Despite the lack of the influence on the drying course in the ethanol-immersed carrot, an increase of the carotenoid content (up to 135%) and the rehydration ability (up to 19%) was noted with the simultaneously unchanged colour of dried carrot in comparison to untreated dried material, which indicates the possibility to improve the quality of dried carrot after immersion in ethanol.
On the Potential of Taxonomic Graphs to Improve Applicability and Performance for the Classification of Biomedical Patents
Authors: Frerich Kai , Bukowski Mark , Geisler Sandra , Farkas Robert
Published: January 2021

A core task in technology management in biomedical engineering and beyond is the classification of patents into domain-specific categories, increasingly automated by machine learning, with the fuzzy language of patents causing particular problems. Striving for higher classification performance, increasingly complex models have been developed, based not only on text but also on a wealth of distinct (meta) data and methods. However, this makes it difficult to access and integrate data and to fuse distinct predictions. Although the already established Cooperate Patent Classification (CPC) offers a plethora of information, it is rarely used in automated patent categorization. Thus, we combine taxonomic and textual information to an ensemble classification system comparing stacking and fixed combination rules as fusion methods. Various classifiers are trained on title/abstract and on both the CPC and IPC (International Patent Classification) assignments of 1230 patents covering six categories of future biomedical innovation. The taxonomies are modeled as tree graphs, parsed and transformed by Dissimilarity Space Embedding (DSE) to real-valued vectors. The classifier ensemble tops the basic performance by nearly 10 points to F1 = 78.7% when stacked with a feed-forward Artificial Neural Network (ANN). Taxonomic base classifiers perform nearly as well as the text-based learners. Moreover, an ensemble only of CPC and IPC learners reaches F1 = 71.2% as fully language independent and straightforward approach of established algorithms and readily available integrated data enabling new possibilities for technology management.
Implementation of a Novel Inertial Mass System and Comparison to Existing Mass-Rig Systems for Shake Table Experiments
Authors: Lopez Alvaro , Dusicka Peter
Published: January 2021

Shake table testing is one of the more effective experimental approaches used to study and evaluate seismic performance of structures. Reduced-scale models can still result in large-scale specimens where incorporating the required inertial mass effectively and safely can be challenging. This study proposes a new system of arranging the mass in the experiments that combines the realism of mass participation during earthquake excitation when supported by the shake table with laboratory practicality considerations of the mass positioned off the specimen. The characteristics and dynamic motion equations for the proposed system are described and applied to shake table experiments involving large-scale cantilevered columns. Using data from large-scale experiments to validate a numerical model, the proposed approach was numerically compared to two other testing approaches. Based on the measured performance and the validated numerical simulations, it can be concluded that the proposed inertial mass system can result in seismic performance as if the mass was placed directly on top of the specimen. Combined with the advantages of reduced setup time, incorporating safety restraints and direct measurement of inertial loads, the proposed system can be suitably used for effective shake table testing of large-scale specimens taken to non-linear near-collapse performance levels.
Calculation of NH3 Emissions, Evaluation of Backward Lagrangian Stochastic Dispersion Model and Aerodynamic Gradient Method
Authors: Kamp Jesper Nørlem , Häni Christoph , Nyord Tavs , Feilberg Anders , Sørensen Lise Lotte
Published: January 2021

Two campaigns measuring ammonia (NH3) emissions with different measurement techniques were performed on a large grass field (26 ha) after the application of liquid animal manure. The aim was to compare emissions from a confined area estimated from either (i) concentration measurements, both point and line-integrated measurements, combined with backward Lagrangian stochastic (bLS) dispersion modeling or by (ii) estimation of the vertical flux by the aerodynamic gradient method (AGM) with and without footprint correction approximated by the bLS model estimates of the flux footprint. The objective of the comparison is to establish the best practice to derive NH3 emissions from a large field. NH3 emissions derived from bLS agreed well when comparing point and line-integrated measurements. Simple point measurements combined with bLS yield good emission estimations for the confined area. Without footprint correction, the AGM underestimates the emissions by up to 9% compared to the footprint-corrected AGM results. The sensitivity of the measurement methods makes it possible to quantify NH3 emissions with diurnal patterns even five days after a field application of liquid animal manure under wet conditions. The bLS model proves to be a strong tool to determine the NH3 emissions from point concentration measurements inside a large field after a slurry application.
Short-Term Aerial Pollutant Concentrations in a Southwestern China Pig-Fattening House
Authors: Pu Shihua , Rong Xiao , Zhu Jiaming , Zeng Yaqiong , Yue Jian , Lim TengTeeh , Long Dingbiao
Published: January 2021

Concentrations of critical aerial pollutants within animal farms are important to the health of animals and farm staff and can be reduced via manure management, ventilation control, and barn design. This study characterized measurements of ammonia (NH3), total suspended particle (TSP), and airborne microbial communities of a large-scale pig-fattening house, as well as their correlations with environmental variables in Southwestern China. Monitoring was conducted for 15 consecutive days during both August and January, at various locations inside the pig house. The concentrations of NH3 and TSP averaged 3.22 and 0.55 mg m−3, respectively, while the average number of airborne microbial colonies was 3.91 log cfu m−3. The aerial pollutant concentrations displayed significant seasonal differences (p < 0.05). Specifically, concentrations in winter were significantly higher than those in summer (p < 0.05), and the 07:00 measurements were the highest among the three measurement times. The concentrations were significantly correlated with indoor temperature and relative humidity. In summer, TSP concentration was negatively correlated with temperature (correlation coefficient = −0.732), while NH3 concentration was positively correlated with temperature (correlation coefficient = 0.58). In winter, TSP and NH3 concentrations were negatively correlated with relative humidity (correlation coefficients = −0.739 and −0.713, respectively), while the airborne microbial colonies were not correlated with either humidity or temperature in summer or winter. These findings confirm that the aerial pollutant concentrations in a Southwestern China pig-fattening house exhibited significant seasonal and diurnal variations. Air quality can be improved by more precise ventilation control as observed by the correlation of concentrations with ventilation control, indoor temperature, and humidity.
A Computational Methodology for the Calibration of Tephra Transport Nowcasting at Sakurajima Volcano, Japan
Authors: Poulidis Alexandros P. , Shimizu Atsushi , Nakamichi Haruhisa , Iguchi Masato
Published: January 2021

Ground-based remote sensing equipment have the potential to be used for the nowcasting of the tephra hazard from volcanic eruptions. To do so raw data from the equipment first need to be accurately transformed to tephra-related physical quantities. In order to establish these relations for Sakurajima volcano, Japan, we propose a methodology based on high-resolution simulations. An eruption that occurred at Sakurajima on 16 July 2018 is used as the basis of a pilot study. The westwards dispersal of the tephra cloud was ideal for the observation network that has been installed near the volcano. In total, the plume and subsequent tephra cloud were recorded by 2 XMP radars, 1 lidar and 3 optical disdrometers, providing insight on all phases of the eruption, from plume generation to tephra transport away from the volcano. The Weather Research and Forecasting (WRF) and FALL3D models were used to reconstruct the transport and deposition patterns. Simulated airborne tephra concentration and accumulated load were linked, respectively, to lidar backscatter intensity and radar reflectivity. Overall, results highlight the possibility of using such a high-resolution modelling-based methodology as a reliable complementary strategy to common approaches for retrieving tephra-related quantities from remote sensing data.
Effects of the Nail Geometry and Humidity on the Nail Penetration of High-Energy Density Lithium Ion Batteries
Authors: Doose Stefan , Haselrieder Wolfgang , Kwade Arno
Published: January 2021

Internal short-circuit tests were carried out in a battery safety investigation chamber to determine the behavior of batteries during the nail penetration test. So far, systematic investigations regarding the test setup and its influence are rarely found in the literature. Especially, to improve the comparability of the multitude of available results, it is essential to understand the effects of the geometric, operating and ambient parameters. In this study commercial lithium ion batteries with a capacity of 5.3 and 3.3 Ah were used to study the influence of the varied parameters on the voltage drop, the development of surface temperatures and of infrared active gas species. We studied both the influence of the geometry of the penetrating nail and concentration of water in the inert atmosphere especially on the quantities of the reaction products under variation of cell capacity. It could be shown that the geometry of the nail, within certain limits, has no influence on the processes of the thermal runaway of high energy density lithium ion batteries (LIBs). However, a change in capacity from 5.3 to 3.3 Ah shows that in particular the gaseous reaction products differ: The standardized gas concentrations show a higher measurable concentration of all gases except CO for the 3.3 Ah LIBs. This circumstance can be explained by the intensity of the reactions due to the different battery capacities: In the 5.3 Ah cells a larger amount of unreacted material is immediately discharged from the reaction center, and by the different available amounts of oxidizing reaction partners. An increase of the water content in the surrounding atmosphere during the thermal runaway leads to a reduction of the measurable gas concentrations of up to 36.01%. In general, all measured concentrations decrease. With increased water content more reaction products from the atmosphere can be directly bound or settle as condensate on surfaces.
In Situ “Humanization” of Porcine Bioprostheses: Demonstration of Tendon Bioprostheses Conversion into Human ACL and Possible Implications for Heart Valve Bioprostheses
Authors: Galili Uri , Stone Kevin R.
Published: January 2021

This review describes the first studies on successful conversion of porcine soft-tissue bioprostheses into viable permanently functional tissue in humans. This process includes gradual degradation of the porcine tissue, with concomitant neo-vascularization and reconstruction of the implanted bioprosthesis with human cells and extracellular matrix. Such a reconstruction process is referred to in this review as “humanization”. Humanization was achieved with porcine bone-patellar-tendon-bone (BTB), replacing torn anterior-cruciate-ligament (ACL) in patients. In addition to its possible use in orthopedic surgery, it is suggested that this humanization method should be studied as a possible mechanism for converting implanted porcine bioprosthetic heart-valves (BHV) into viable tissue valves in young patients. Presently, these patients are only implanted with mechanical heart-valves, which require constant anticoagulation therapy. The processing of porcine bioprostheses, which enables humanization, includes elimination of α-gal epitopes and partial (incomplete) crosslinking with glutaraldehyde. Studies on implantation of porcine BTB bioprostheses indicated that enzymatic elimination of α-gal epitopes prevents subsequent accelerated destruction of implanted tissues by the natural anti-Gal antibody, whereas the partial crosslinking by glutaraldehyde molecules results in their function as “speed bumps” that slow the infiltration of macrophages. Anti-non gal antibodies produced against porcine antigens in implanted bioprostheses recruit macrophages, which infiltrate at a pace that enables slow degradation of the porcine tissue, neo-vascularization, and infiltration of fibroblasts. These fibroblasts align with the porcine collagen-fibers scaffold, secrete their collagen-fibers and other extracellular-matrix (ECM) components, and gradually replace porcine tissues degraded by macrophages with autologous functional viable tissue. Porcine BTB implanted in patients completes humanization into autologous ACL within ~2 years. The similarities in cells and ECM comprising heart-valves and tendons, raises the possibility that porcine BHV undergoing a similar processing, may also undergo humanization, resulting in formation of an autologous, viable, permanently functional, non-calcifying heart-valves.
Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats
Authors: Klancic Teja , Laforest-Lapointe Isabelle , Wong Jolene , Choo Ashley , Nettleton Jodi E. , Chleilat Faye , Arrieta Marie-Claire , Reimer Raylene A.
Published: January 2021

Pulsed antibiotic treatment (PAT) early in life increases risk of obesity. Prebiotics can reduce fat mass and improve metabolic health. We examined if co-administering prebiotic with PAT reduces obesity risk in rat pups weaned onto a high fat/sucrose diet. Pups were randomized to (1) control [CTR], (2) antibiotic [ABT] (azithromycin), (3) prebiotic [PRE] (10% oligofructose (OFS)), (4) antibiotic + prebiotic [ABT + PRE]. Pulses of antibiotics/prebiotics were administered at d19–21, d28–30 and d37–39. Male and female rats given antibiotics (ABT) had higher body weight than all other groups at 10 wk of age. The PAT phenotype was stronger in ABT males than females, where increased fat mass, hyperinsulinemia and insulin resistance were present and all reversible with prebiotics. Reduced hypothalamic and hepatic expression of insulin receptor substrates and ileal tight junction proteins was seen in males only, explaining their greater insulin resistance. In females, insulin resistance was improved with prebiotics and normalized to lean control. ABT reduced Lactobacillaceae and increased Bacteroidaceae in both sexes. Using a therapeutic dose of an antibiotic commonly used for acute infection in children, PAT increased body weight and impaired insulin production and insulin sensitivity. The effects were reversed with prebiotic co-administration in a sex-specific manner.
Plumericin Protects against Experimental Inflammatory Bowel Disease by Restoring Intestinal Barrier Function and Reducing Apoptosis
Authors: Rapa Shara Francesca , Di Paola Rosanna , Cordaro Marika , Siracusa Rosalba , D’Amico Ramona , Fusco Roberta , Autore Giuseppina , Cuzzocrea Salvatore , Stuppner Hermann , Marzocco Stefania
Published: January 2021

Intestinal epithelial barrier impairment plays a key pathogenic role in inflammatory bowel diseases (IBDs). In particular, together with oxidative stress, intestinal epithelial barrier alteration is considered as upstream event in ulcerative colitis (UC). In order to identify new products of natural origin with a potential activity for UC treatment, this study evaluated the effects of plumericin, a spirolactone iridoid, present as one of the main bioactive components in the bark of Himatanthus sucuuba (Woodson). Plumericin was evaluated for its ability to improve barrier function and to reduce apoptotic parameters during inflammation, both in intestinal epithelial cells (IEC-6), and in an animal experimental model of 2, 4, 6-dinitrobenzene sulfonic acid (DNBS)-induced colitis. Our results indicated that plumericin increased the expression of adhesion molecules, enhanced IEC-6 cells actin cytoskeleton rearrangement, and promoted their motility. Moreover, plumericin reduced apoptotic parameters in IEC-6. These results were confirmed in vivo. Plumericin reduced the activity of myeloperoxidase, inhibited the expression of ICAM-1, P-selectin, and the formation of PAR, and reduced apoptosis parameters in mice colitis induced by DNBS. These results support a pharmacological potential of plumericin in the treatment of UC, due to its ability to improve the structural integrity of the intestinal epithelium and its barrier function.
Mithramycin and Analogs for Overcoming Cisplatin Resistance in Ovarian Cancer
Authors: Schweer David , McCorkle J. Robert , Rohr Jurgen , Tsodikov Oleg V. , Ueland Frederick , Kolesar Jill
Published: January 2021

Ovarian cancer is a highly deadly malignancy in which recurrence is considered incurable. Resistance to platinum-based chemotherapy bodes a particularly abysmal prognosis, underscoring the need for novel therapeutic agents and strategies. The use of mithramycin, an antineoplastic antibiotic, has been previously limited by its narrow therapeutic window. Recent advances in semisynthetic methods have led to mithramycin analogs with improved pharmacological profiles. Mithramycin inhibits the activity of the transcription factor Sp1, which is closely linked with ovarian tumorigenesis and platinum-resistance. This article summarizes recent clinical developments related to mithramycin and postulates a role for the use of mithramycin, or its analog, in the treatment of platinum-resistant ovarian cancer.
Identification of Sclerostin as a Putative New Myokine Involved in the Muscle-to-Bone Crosstalk
Authors: Magarò Maria Sara , Bertacchini Jessika , Florio Francesca , Zavatti Manuela , Potì Francesco , Cavani Francesco , Amore Emanuela , De Santis Ilaria , Bevilacqua Alessandro , Reggiani Bonetti Luca , Torricelli Pietro , Maurel Delphine B. , Biressi Stefano , Palumbo Carla
Published: January 2021

Bone and muscle have been recognized as endocrine organs since they produce and secrete “hormone-like factors” that can mutually influence each other and other tissues, giving rise to a “bone–muscle crosstalk”. In our study, we made use of myogenic (C2C12 cells) and osteogenic (2T3 cells) cell lines to investigate the effects of muscle cell-produced factors on the maturation process of osteoblasts. We found that the myogenic medium has inhibitory effects on bone cell differentiation and we identified sclerostin as one of the myokines produced by muscle cells. Sclerostin is a secreted glycoprotein reportedly expressed by bone/cartilage cells and is considered a negative regulator of bone growth due to its role as an antagonist of the Wnt/β-catenin pathway. Given the inhibitory role of sclerostin in bone, we analyzed its expression by muscle cells and how it affects bone formation and homeostasis. Firstly, we characterized and quantified sclerostin synthesis by a myoblast cell line (C2C12) and by murine primary muscle cells by Western blotting, real-time PCR, immunofluorescence, and ELISA assay. Next, we investigated in vivo production of sclerostin in distinct muscle groups with different metabolic and mechanical loading characteristics. This analysis was done in mice of different ages (6 weeks, 5 and 18 months after birth) and revealed that sclerostin expression is dynamically modulated in a muscle-specific way during the lifespan. Finally, we transiently expressed sclerostin in the hind limb muscles of young mice (2 weeks of age) via in vivo electro-transfer of a plasmid containing the SOST gene in order to investigate the effects of muscle-specific overproduction of the protein. Our data disclosed an inhibitory role of the muscular sclerostin on the bones adjacent to the electroporated muscles. This observation suggests that sclerostin released by skeletal muscle might synergistically interact with osseous sclerostin and potentiate negative regulation of osteogenesis possibly by acting in a paracrine/local fashion. Our data point out a role for muscle as a new source of sclerostin.
KCNA2 Autoimmunity in Progressive Cognitive Impairment: Case Series and Literature Review
Authors: Timäus Charles , von Gottberg Philipp , Hirschel Sina , Lange Claudia , Wiltfang Jens , Hansen Niels
Published: January 2021

Autoimmune dementia is a novel and expanding field which subsumes neuropsychiatric disorders with predominant cognitive impairments due to an underlying autoimmune etiology. Progressive dementias with atypical clinical presentation should trigger a thorough diagnostic approach including testing for neural surface and intracellular antibodies to avoid a delay in accurate diagnosis and initiating appropriate therapy. Here, we present two emerging cases of progressive dementia with co-existing serum autoantibodies against the KCNA2 (potassium voltage-gated channel subfamily A member 2) subunit. We found various cognitive deficits with dominant impairments in the memory domain, particularly in delayed recall. One patient presented a subacute onset of then-persisting cognitive deficits, while the other patient’s cognitive impairments progressed more chronically and fluctuated. Cognitive impairments coincided with additional neuropsychiatric symptoms. Both had a potential paraneoplastic background according to their medical history and diagnostic results. We discuss the potential role of KCNA2 autoantibodies in these patients and in general by reviewing the literature. The pathogenetic role of KCNA2 antibodies in cognitive impairment is not well delineated; clinical presentations are heterogeneous, and thus a causal link between antibodies remains questionable. Current evidence indicates an intracellular rather than extracellular epitope. We strongly suggest additional prospective studies to explore KCNA2 antibodies in specifically-defined cohorts of cognitively impaired patients via a systematic assessment of clinical, neuropsychological, neuroimaging, as well as laboratory and CSF (cerebrospinal fluid) parameters, and antibody studies to (1) determine the epitope’s location (intracellular vs. extracellular), (2) the mode of action, and (3) seek co-existing, novel pathogenetic autoantibodies in sera and CSF.
Structural Behavior of Reinforced Concrete Slabs Containing Fine Waste Aggregates of Polyvinyl Chloride
Authors: Mohammed Nisreen S. , Hamza Bashar Abid , AL-Shareef Najla’a H. , Hussein Husam H.
Published: January 2021

In several areas worldwide, the high cost and shortage of natural resources have encouraged researchers and engineers to explore the serviceability and feasibility of using recycled aggregates in concrete mixtures, substituting a normal aggregate percentage. This technique has advantages for the environment by reducing the accumulation of waste materials, while it impacts the fresh and hardened concrete performances, reducing workability, flexural strength, compressive strength, and tensile strength. However, most studies have investigated the influence of replacing normal aggregates with waste aggregates on the concrete mechanical properties without examining the impact of using waste materials on concrete structural performance. The aim of this research is to investigate the effect of replacing 75% of sand volume with polyvinyl chloride (PVC) fine waste aggregates on the performance of reinforced concrete slabs. Different thicknesses of the concrete layer (0%, 25%, 50%, and 100% of slab thickness) containing PVC fine waste aggregates are investigated. Based on the reductions in the toughness and flexural strength capacity due to incorporating 75% PVC fine aggregate dosage, two approaches are used to strengthen the slabs with 75% PVC fine aggregates. The first approach is adding polyvinyl alcohol (PVA) to the PVC fine aggregate concrete mix to improve the mechanical properties of the concrete. The PVA increases the water viscosity in the concrete, which reduces the dry out phenomenon. With that said, the PVA modified fresh concrete does enable the use of the limits of the PVC fine aggregate dosage for high dosage plastic aggregate concrete. The second approach uses two fiber wire mesh layers as an additional reinforcement in the tested slab. Results show that the PVC-30 slab exhibits an 8% decrease in total area toughness compared to the control (Con) slab, while for PVC-60 slab toughness, the total area shows 26% less. Additionally, the inclusion of PVA in the concrete with 75% PVC plastic waste fine aggregate replacement greatly influences the pre-and post-cracking ductile performance among other slabs, representing that using PVA with higher contents might increase the flexural performance. Therefore, due to the substantial effect of PVA material on the concrete flexural performance, it is proposed to utilize PVA with an optimum PCV fine aggregate dosage in the concrete mix.
DNA-Damage-Induced Alternative Splicing of p53
Authors: Chen Jing , Zhang Dadong , Qin Xiaodi , Owzar Kouros , McCann Jennifer J. , Kastan Michael B.
Published: January 2021

Cellular responses to DNA damage and other stresses are important determinants of mutagenesis and impact the development of a wide range of human diseases. TP53 is highly mutated in human cancers and plays an essential role in stress responses and cell fate determination. A central dogma of p53 induction after DNA damage has been that the induction results from a transient increase in the half-life of the p53 protein. Our laboratory recently demonstrated that this long-standing paradigm is an incomplete picture of p53 regulation by uncovering a critical role for protein translational regulation in p53 induction after DNA damage. These investigations led to the discovery of a DNA-damage-induced alternative splicing (AS) pathway that affects p53 and other gene products. The damage-induced AS of p53 pre-mRNA generates the beta isoform of p53 (p53β) RNA and protein, which is specifically required for the induction of cellular senescence markers after ionizing irradiation (IR). In an attempt to elucidate the mechanisms behind the differential regulation and apparent functional divergence between full-length (FL) p53 and the p53β isoform (apoptosis versus senescence, respectively), we identified the differential transcriptome and protein interactome between these two proteins that may result from the unique 10-amino-acid tail in p53β protein.
Epigenetic Modulation of SPCA2 Reverses Epithelial to Mesenchymal Transition in Breast Cancer Cells
Authors: Makena Monish Ram , Ko Myungjun , Dang Donna Kimberly , Rao Rajini
Published: January 2021

The secretory pathway Ca2+-ATPase SPCA2 is a tumor suppressor in triple receptor negative breast cancer (TNBC), a highly aggressive molecular subtype that lacks tailored treatment options. Low expression of SPCA2 in TNBC confers poor survival prognosis in patients. Previous work has established that re-introducing SPCA2 to TNBC cells restores basal Ca2+ signaling, represses mesenchymal gene expression, mitigates tumor migration in vitro and metastasis in vivo. In this study, we examined the effect of histone deacetylase inhibitors (HDACi) in TNBC cell lines. We show that the pan-HDACi vorinostat and the class I HDACi romidepsin induce dose-dependent upregulation of SPCA2 transcript with concurrent downregulation of mesenchymal markers and tumor cell migration characteristic of epithelial phenotype. Silencing SPCA2 abolished the ability of HDACi to reverse epithelial to mesenchymal transition (EMT). Independent of ATPase activity, SPCA2 elevated resting Ca2+ levels to activate downstream components of non-canonical Wnt/Ca2+ signaling. HDACi treatment led to SPCA2-dependent phosphorylation of CAMKII and β-catenin, turning Wnt signaling off. We conclude that SPCA2 mediates the efficacy of HDACi in reversing EMT in TNBC by a novel mode of non-canonical Wnt/Ca2+ signaling. Our findings provide incentive for screening epigenetic modulators that exploit Ca2+ signaling pathways to reverse EMT in breast tumors.
An Emerging Role for the Unfolded Protein Response in Pancreatic Cancer
Authors: Robinson Claire M. , Talty Aaron , Logue Susan E. , Mnich Katarzyna , Gorman Adrienne M. , Samali Afshin
Published: January 2021

Pancreatic ductal adenocarcinoma (PDAC) is the most common form of pancreatic cancer and one of the leading causes of cancer-associated deaths in the world. It is characterised by dismal response rates to conventional therapies. A major challenge in treatment strategies for PDAC is the presence of a dense stroma that surrounds the tumour cells, shielding them from treatment. This unique tumour microenvironment is fuelled by paracrine signalling between pancreatic cancer cells and supporting stromal cell types including the pancreatic stellate cells (PSC). While our molecular understanding of PDAC is improving, there remains a vital need to develop effective, targeted treatments. The unfolded protein response (UPR) is an elaborate signalling network that governs the cellular response to perturbed protein homeostasis in the endoplasmic reticulum (ER) lumen. There is growing evidence that the UPR is constitutively active in PDAC and may contribute to the disease progression and the acquisition of resistance to therapy. Given the importance of the tumour microenvironment and cytokine signalling in PDAC, and an emerging role for the UPR in shaping the tumour microenvironment and in the regulation of cytokines in other cancer types, this review explores the importance of the UPR in PDAC biology and its potential as a therapeutic target in this disease.
Characterization of SOX2, OCT4 and NANOG in Ovarian Cancer Tumor-Initiating Cells
Authors: Robinson Mikella , Gilbert Samuel F. , Waters Jennifer A. , Lujano-Olazaba Omar , Lara Jacqueline , Alexander Logan J. , Green Samuel E. , Burkeen Gregory A. , Patrus Omid , Sarwar Zinia , Holmberg Ryne , Wang Christine , House Carrie D.
Published: January 2021

The identification of tumor-initiating cells (TICs) has traditionally relied on surface markers including CD133, CD44, CD117, and the aldehyde dehydrogenase (ALDH) enzyme, which have diverse expression across samples. A more reliable indication of TICs may include the expression of embryonic transcription factors that support long-term self-renewal, multipotency, and quiescence. We hypothesize that SOX2, OCT4, and NANOG will be enriched in ovarian TICs and may indicate TICs with high relapse potential. We evaluated a panel of eight ovarian cancer cell lines grown in standard 2-D culture or in spheroid-enriching 3-D culture, and correlated expression with growth characteristics, TIC marker expression, and chemotherapy resistance. RNA-sequencing showed that cell cycle regulation pathways involving SOX2 were elevated in 3-D conditions. HGSOC lines had longer doubling-times, greater chemoresistance, and significantly increased expression of SOX2, OCT4, and NANOG in 3-D conditions. CD117+ or ALDH+/CD133+ cells had increased SOX2, OCT4, and NANOG expression. Limiting dilution in in vivo experiments implicated SOX2, but not OCT4 or NANOG, with early tumor-initiation. An analysis of patient data suggested a stronger role for SOX2, relative to OCT4 or NANOG, for tumor relapse potential. Overall, our findings suggest that SOX2 may be a more consistent indicator of ovarian TICs that contribute to tumor repopulation following chemotherapy. Future studies evaluating SOX2 in TIC biology will increase our understanding of the mechanisms that drive ovarian cancer relapse.
Evidence-Based Exercise Recommendations to Improve Mental Wellbeing in Women with Breast Cancer during Active Treatment: A Systematic Review and Meta-Analysis
Authors: Ramírez-Vélez Robinson , Zambom-Ferraresi Fabiola , García-Hermoso Antonio , Kievisiene Justina , Rauckiene-Michealsson Alona , Agostinis-Sobrinho César
Published: January 2021

Breast cancer was the most common cancer in women worldwide. The aims of the current systematic review and meta-analysis are: (i) to systematically examine the effects of exercise interventions on mental wellbeing; (ii) to examine the specific effect of the type of supervised exercise and its intensity, volume and frequency on mental wellbeing; and (iii) to explore which interventions are most effective in mental wellbeing among women with breast cancer during active treatment. An electronic literature search was performed using MEDLINE (via PubMed), Embase (Ovid), and Web of Science, we identified 175 full-text articles. The 57 publications included data from 6988 participants, age ranging from 18 to 78 years (weighted mean: 50.85 years). Compared with the control conditions, exercise training programs were associated with significant reductions in anxiety (d = −0.22, I2 = 53.0%), depression (d = −0.24, I2 = 66.6%), and fatigue (d = −0.47, I2 = 69.8%), as well as increases in body image (d = 0.27, I2 = 69.2%) and quality of life (overall, d = 0.46, I2 = 71.6%; emotional function, d = 0.33, I2 = 65.7%; and FACT-B, d = 0.60, I2 = 76.2%). There were a variety of frequencies, intensities, and durations of supervised exercise programs reported in the included meta-analytic approach. In addition, we found that concomitant concurrent training, at moderate-to-vigorous intensity, and with a volume ≥50 min/week had benefits on a number of health outcomes, such as fatigue, depression, and quality of life measure by the FACT-B instrument. These findings have important implications for healthcare providers and multidisciplinary teams involved in mental health management in cancer patients during active treatment.
Cobalt-Based Metal Organic Frameworks as Solids Catalysts for Oxidation Reactions
Authors: Dhakshinamoorthy Amarajothi , Montero Lanzuela Eva , Navalon Sergio , Garcia Hermenegildo
Published: January 2021

Metal organic frameworks (MOFs) are porous crystalline solids whose frameworks are constituted by metal ions/nodes with rigid organic linkers leading to the formation of materials having high surface area and pore volume. One of the unique features of MOFs is the presence of coordinatively unsaturated metal sites in their crystalline lattice that can act as Lewis acid sites promoting organic transformations, including aerobic oxidation reactions of various substrates such as hydrocarbons, alcohols, and sulfides. This review article summarizes the existing Co-based MOFs for oxidation reactions organized according to the nature of substrates like hydrocarbon, alcohol, olefin, and water. Both aerobic conditions and peroxide oxidants are discussed. Emphasis is placed on comparing the advantages of using MOFs as solid catalysts with respect to homogeneous salts in terms of product selectivity and long-term stability. The final section provides our view on future developments in this field.
Chronicle of Nanocelluloses (NCs) for Catalytic Applications: Key Advances
Authors: Maiuolo Loredana , Algieri Vincenzo , Olivito Fabrizio , Tallarida Matteo Antonio , Costanzo Paola , Jiritano Antonio , De Nino Antonio
Published: January 2021

Nanocellulose (NC) is a biomaterial with growing interest in the field of nanocomposites and sustainable materials. NC has various applications including biodegradable materials, reinforcing agents, packaging films, transpiring membranes and medical devices. Among the many applications, the use of NC functionalized with organic and inorganic groups has found wide use as a catalyst in chemical transformations. The goal of this review is to collect the current knowledge on its catalytic applications for chemical groups conversion. We have chosen to organize the manuscript according to subdivision of NC into Bacterial Nanocellulose (BNC), Cellulose Nanocrystals (CNCs), and Cellulose Nanofibers (CNFs) and their role as inorganic- and organic-functionalized NC-catalysts in organic synthesis. However, in consideration of the fact that the literature on this field is very extensive, we have decided to focus our attention on the scientific productions of the last five years.
Apoptosis in the Extraosseous Calcification Process
Authors: Boraldi Federica , Lofaro Francesco Demetrio , Quaglino Daniela
Published: January 2021

Extraosseous calcification is a pathologic mineralization process occurring in soft connective tissues (e.g., skin, vessels, tendons, and cartilage). It can take place on a genetic basis or as a consequence of acquired chronic diseases. In this last case, the etiology is multifactorial, including both extra- and intracellular mechanisms, such as the formation of membrane vesicles (e.g., matrix vesicles and apoptotic bodies), mitochondrial alterations, and oxidative stress. This review is an overview of extraosseous calcification mechanisms focusing on the relationships between apoptosis and mineralization in cartilage and vascular tissues, as these are the two tissues mostly affected by a number of age-related diseases having a progressively increased impact in Western Countries.
Radiation-Activated PI3K/AKT Pathway Promotes the Induction of Cancer Stem-Like Cells via the Upregulation of SOX2 in Colorectal Cancer
Authors: Park Ji-Hye , Kim Young-Heon , Shim Sehwan , Kim Areumnuri , Jang Hyosun , Lee Su-Jae , Park Sunhoo , Seo Songwon , Jang Won Il , Lee Seung Bum , Kim Min-Jung
Published: January 2021

The current treatment strategy for patients with aggressive colorectal cancer has been hampered by resistance to radiotherapy and chemotherapy due to the existence of cancer stem-like cells (CSCs). Recent studies have shown that SOX2 expression plays an important role in the maintenance of CSC properties in colorectal cancer. In this study, we investigated the induction and regulatory role of SOX2 following the irradiation of radioresistant and radiosensitive colorectal cancer cells. We used FACS and western blotting to analyze SOX2 expression in cells. Among the markers of colorectal CSCs, the expression of CD44 increased upon irradiation in radioresistant cells. Further analysis revealed the retention of CSC properties with an upregulation of SOX2 as shown by enhanced resistance to radiation and metastatic potential in vitro. Interestingly, both the knockdown and overexpression of SOX2 led to increase in CD44+ population and induction of CSC properties in colorectal cancer following irradiation. Furthermore, selective genetic and pharmacological inhibition of the PI3K/AKT pathway, but not the MAPK pathway, attenuated SOX2-dependent CD44 expression and metastatic potential upon irradiation in vitro. Our findings suggested that SOX2 regulated by radiation-induced activation of PI3K/AKT pathway contributes to the induction of colorectal CSCs, thereby highlighting its potential as a therapeutic target.
Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation
Authors: Nilsson Fredrik , Storm Petter , Sozzi Edoardo , Hidalgo Gil David , Birtele Marcella , Sharma Yogita , Parmar Malin , Fiorenzano Alessandro
Published: January 2021

Dopaminergic (DA) neurons derived from human pluripotent stem cells (hPSCs) represent a renewable and available source of cells useful for understanding development, developing disease models, and stem-cell therapies for Parkinson’s disease (PD). To assess the utility of stem cell cultures as an in vitro model system of human DA neurogenesis, we performed high-throughput transcriptional profiling of ~20,000 ventral midbrain (VM)-patterned stem cells at different stages of maturation using droplet-based single-cell RNA sequencing (scRNAseq). Using this dataset, we defined the cellular composition of human VM cultures at different timepoints and found high purity DA progenitor formation at an early stage of differentiation. DA neurons sharing similar molecular identities to those found in authentic DA neurons derived from human fetal VM were the major cell type after two months in culture. We also developed a bioinformatic pipeline that provided a comprehensive long noncoding RNA landscape based on temporal and cell-type specificity, which may contribute to unraveling the intricate regulatory network of coding and noncoding genes in DA neuron differentiation. Our findings serve as a valuable resource to elucidate the molecular steps of development, maturation, and function of human DA neurons, and to identify novel candidate coding and noncoding genes driving specification of progenitors into functionally mature DA neurons.
Age- and Sex-Related Differences in Morbidities of Sexually Transmitted Diseases in Children
Authors: Hino Yumika , Eshima Nobuoki , Bacal Kira , Tokumaru Osamu
Published: January 2021

Sexually transmitted diseases (STDs) are causes of public health burden globally. The purpose of this study is to document age-specific and sex-related changes in the morbidity of four representative STDs in children. Japanese national surveillance data from 1999 to 2017 on morbidities of the following four STDs were analyzed by age and sex: Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), condylomata acuminate (CA), and genital herpes simplex virus (GHSV). The morbidities of males and females in each age group were compared through the male-to-female morbidity (MFM) ratios. The MFM ratios were not different from one in infants, less than one in children, and greater than one after puberty in all four STDs. The reversal of MFM ratio less than 1 to greater than 1 for NG infection was observed between 10–14 and 15–19 year of age, i.e., during the puberty, while that for GHSV infection was observed between 35–39 and 40–44 year of age, i.e., during adulthood. In conclusion, the morbidities of the four STDs were similar between the sexes in infants, and were higher in female children than in male children, while the morbidities in all four diseases were higher in men after puberty.
Motor Development among Spanish Preschool Children
Authors: Honrubia-Montesinos Cristina , Gil-Madrona Pedro , Losada-Puente Luisa
Published: January 2021

The purpose of this study was to analyze motor development of Spanish preschoolers, taking into account sex and age, being an only child, prematurity, and the practice of extracurricular activities. The sample was composed of 300 preschoolers (132 girls, 168 boys) ages 3 to 6 years. Preschoolers were tested on 12 fundamental motor skills (locomotor and object control) through the Test of Gross Motor Development—Second Edition (TGMD-2). Nonparametric analysis indicated that there are differences between girls and boys in locomotor and object control skills in the age range of 3–4 years. However, boys and girls scored similarly at the age of 5 years in locomotor development. There were not differences between only children and those who are not only children. Similarly, prematurity was not associated with locomotor and object control development. Nevertheless, those preschoolers who practice extracurricular physical activities scored significantly higher in comparison with those children do not. Further research is needed to shed light on the differences between boys and girls in object control. It may be explained by the types of extracurricular activities.
A Review on the Electrodeposition of Aluminum and Aluminum Alloys in Ionic Liquids
Authors: Maniam Kranthi Kumar , Paul Shiladitya
Published: January 2021

Aluminum plays an essential role as an excellent coating material in diversified applications due to its better corrosion resistance and physicochemical properties. Employing such a material as a coating on different metallic substrates such as carbon steel would benefit many industries such as the automotive, aviation, shipbuilding, construction, electronics etc. Amongst the various available coating techniques, electrodeposition of aluminum (Al) Al alloys have gained significant attention in the last 10 years as a metallic protection coating for various commercial substrates and has become the industry’s choice owing to being lightweight, corrosion-resistant, and cost-effective. This paper shall provide a detailed review covering electrochemical deposition of Al and Al alloys using ionic liquids with various cations, anions, and additives, and reports on progress in development thus far. It shall also cover the challenges in the electrodepositing aluminum, its alloys on light weight metal substrates viz., magnesium (Mg), commercial substrates such as low carbon steel, spring steel, and their pretreatments. The factors that play an important role in electroplating on an industrial scale, along with future challenges, are discussed.
Bone Structure Analysis of the Radius Using Ultrahigh Field (7T) MRI: Relevance of Technical Parameters and Comparison with 3T MRI and Radiography
Authors: Jarraya Mohamed , Heiss Rafael , Duryea Jeffrey , Nagel Armin M. , Lynch John A. , Guermazi Ali , Weber Marc-André , Arkudas Andreas , Horch Raymund E. , Uder Michael , Roemer Frank W.
Published: January 2021

Bone fractal signature analysis (FSA—also termed bone texture analysis) is a tool that assesses structural changes that may relate to clinical outcomes and functions. Our aim was to compare bone texture analysis of the distal radius in patients and volunteers using radiography and 3T and 7T magnetic resonance imaging (MRI)—a patient group (n = 25) and a volunteer group (n = 25) were included. Participants in the patient group had a history of chronic wrist pain with suspected or confirmed osteoarthritis and/or ligament instability. All participants had 3T and 7T MRI including T1-weighted turbo spin echo (TSE) sequences. The 7T MRI examination included an additional high-resolution (HR) T1 TSE sequence. Radiographs of the wrist were acquired for the patient group. When comparing patients and volunteers (unadjusted for gender and age), we found a statistically significant difference of horizontal and vertical fractal dimensions (FDs) using 7T T1 TSE-HR images in low-resolution mode (horizontal: p = 0.04, vertical: p = 0.01). When comparing radiography to the different MRI sequences, we found a statistically significant difference for low- and high-resolution horizontal FDs between radiography and 3T T1 TSE and 7T T1 TSE-HR. Vertical FDs were significantly different only between radiographs and 3T T1 TSE in the high-resolution mode; FSA measures obtained from 3T and 7T MRI are highly dependent on the sequence and reconstruction resolution used, and thus are not easily comparable between MRI systems and applied sequences.