bluuebunny/crossref_metadata_2025 huggingface dataset:
Epigenetic and transcriptome responsiveness to ER modulation by tissue selective estrogen complexes in breast epithelial and breast cancer cells
Authors: Messier Terri L. , Boyd Joseph R. , Gordon Jonathan A. R. , Tye Coralee E. , Page Natalie A. , Toor Rabail H. , Zaidi Sayyed K. , Komm Barry S. , Frietze Seth , Stein Janet L. , Lian Jane B. , Stein Gary S.
Published: July 2022

Selective estrogen receptor modulators (SERMs), including the SERM/SERD bazedoxifene (BZA), are used to treat postmenopausal osteoporosis and may reduce breast cancer (BCa) risk. One of the most persistent unresolved questions regarding menopausal hormone therapy is compromised control of proliferation and phenotype because of short- or long-term administration of mixed-function estrogen receptor (ER) ligands. To gain insight into epigenetic effectors of the transcriptomes of hormone and BZA-treated BCa cells, we evaluated a panel of histone modifications. The impact of short-term hormone treatment and BZA on gene expression and genome-wide epigenetic profiles was examined in ERαneg mammary epithelial cells (MCF10A) and ERα+ luminal breast cancer cells (MCF7). We tested individual components and combinations of 17β-estradiol (E2), estrogen compounds (EC10) and BZA. RNA-seq for gene expression and ChIP-seq for active (H3K4me3, H3K4ac, H3K27ac) and repressive (H3K27me3) histone modifications were performed. Our results show that the combination of BZA with E2 or EC10 reduces estrogen-mediated patterns of histone modifications and gene expression in MCF-7ERα+ cells. In contrast, BZA has minimal effects on these parameters in MCF10A mammary epithelial cells. BZA-induced changes in histone modifications in MCF7 cells are characterized by altered H3K4ac patterns, with changes at distal enhancers of ERα-target genes and at promoters of non-ERα bound proliferation-related genes. Notably, the ERα target gene GREB1 is the most sensitive to BZA treatment. Our findings provide direct mechanistic-based evidence that BZA induces epigenetic changes in E2 and EC10 mediated control of ERα regulatory programs to target distinctive proliferation gene pathways that restrain the potential for breast cancer development.
High-frequency ultrasound detects biomechanical weakening in keratoconus with lower stiffness at higher grade
Authors: Kwok Sunny , Pan Xueliang , Liu William , Hendershot Andrew , Liu Jun
Published: July 2022

In vivo biomechanical characterization of the cornea remains a challenge. We have developed a high-frequency ultrasound elastography method, the ocular pulse elastography (OPE), to measure corneal axial displacement (CAD) induced by the ocular pulse. Here we compared CAD and a stiffness index derived from CAD between keratoconus patients and normal controls. We also explored the trend of these parameters with keratoconus grade. Twenty normal subjects and twenty keratoconus patients were recruited in this study. Corneal topography, tomography, intraocular pressure (IOP) and ocular pulse amplitude (OPA) were obtained in each measured eye. The cornea’s heartbeat-induced cyclic axial displacements were measured by high-frequency (50 MHz) ultrasound. A corneal stiffness index (CSI) was derived from CAD normalized against OPA. CAD and CSI were compared between normal and keratoconus groups, and across keratoconus grades. Keratoconus corneas had significantly greater CAD and lower CSI than normal controls (p’s<0.01). Both parameters correlated strongly with grade, in which CAD increased significantly (p = 0.002) and CSI decreased significantly (p = 0.011) with grade. These results suggested a biomechanical weakening in keratoconus which worsens at higher disease severity. This study also demonstrated the ability of high-frequency ultrasound elastography to provide a safe, quick, and accurate evaluation of the cornea’s biomechanical condition in vivo. The OPE-measured biomechanical metrics, when integrated with existing diagnostic criteria, may aid the decision-making in the early and definitive diagnosis and staging of keratoconus.
TBK1 is part of a galectin 8 dependent membrane damage recognition complex and drives autophagy upon Adenovirus endosomal escape
Authors: Pied Noémie , Daussy Coralie F. , Denis Zoé , Ragues Jessica , Faure Muriel , Iggo Richard , Tschan Mario P. , Roger Benoit , Rayne Fabienne , Wodrich Harald
Published: July 2022

Intracellular pathogens cause membrane distortion and damage as they enter host cells. Cells perceive these membrane alterations as danger signals and respond by activating autophagy. This response has primarily been studied during bacterial invasion, and only rarely in viral infections. Here, we investigate the cellular response to membrane damage during adenoviral entry. Adenoviruses and their vector derivatives, that are an important vaccine platform against SARS-CoV-2, enter the host cell by endocytosis followed by lysis of the endosomal membrane. We previously showed that cells mount a locally confined autophagy response at the site of endosomal membrane lysis. Here we describe the mechanism of autophagy induction: endosomal membrane damage activates the kinase TBK1 that accumulates in its phosphorylated form at the penetration site. Activation and recruitment of TBK1 require detection of membrane damage by galectin 8 but occur independently of classical autophagy receptors or functional autophagy. Instead, TBK1 itself promotes subsequent autophagy that adenoviruses need to take control of. Deletion of TBK1 reduces LC3 lipidation during adenovirus infection and restores the infectivity of an adenovirus mutant that is restricted by autophagy. By comparing adenovirus-induced membrane damage to sterile lysosomal damage, we implicate TBK1 in the response to a broader range of types of membrane damage. Our study thus highlights an important role for TBK1 in the cellular response to adenoviral endosome penetration and places TBK1 early in the pathway leading to autophagy in response to membrane damage.
Curating the circus: Collaboration and responsibilities in digital costume exhibitions
Authors: Du Puis Jenny Leigh , Yamakawa Chisato
Published: July 2022

Flights of Fancy: Fashion and Function in Circus Performance is a digital exhibition of circus costumes that examines the complexities, histories and representations of circus through representations of material artefacts, ephemera, print media, photographs and other primary sources. Through curated features of the exhibit, we investigate circus costumes’ sociocultural influence on fashion through aesthetics and functional design. The purpose of this article is to explore how digital fashion exhibitions may be developed to create educational resources and how their curation process may be interpreted through theory. To do this, we examine the curation of Flights of Fancy through an exploration of its use of symbolism as an organizational device. We use multiple theoretical lenses, including Erving Goffman’s concepts of the front and back stage presentations of self, along with Anneke Smelik and Susan Kaiser’s updated view that interprets Goffman in the digital/social media sphere, Paul O’Neill’s composition of exhibitions through background/middle ground/foreground and Susan Kaiser’s time, space and place. Flights of Fancy: Fashion and Function in Circus Performance, digital exhibition (February 2021 – present; Cornell Fashion + Textile Collection, Cornell University Library): https://exhibits.library.cornell.edu/flights-of-fancy.
Digital fashion bodies between the conflicting priorities of media-technological innovations
Authors: Zauner Jacqueline
Published: July 2022

This article presents an insight into my ongoing Ph.D. project ‘New fashion bodies: Digital fashion bodies between the conflicting priorities of media-technological innovations’. The thesis considers how digital technologies have impacted the construction of gender as well as the cultural (body)practices and (body)enactments tied to it via fashion. The relationships and interactions between the body, fashion, media, gender and staging of queerness are at the core of interest. To elaborate and highlight the interdependencies of these categories and their intricate entanglements, this article studies the phenomenon of digital fashion influencers such as Miquela (@lil_miquela), Lil_wavi (@lil_wavi) and the digital fashion bodies created by the Institute of Digital Fashion (@institute_digital_fashion).
Applied Theatre and Sexual Health Communication: Apertures of Possibility, Katharine E. Low (2020)
Authors: Hahn Matthew
Published: July 2022

Review of: Applied Theatre and Sexual Health Communication: Apertures of Possibility, Katharine E. Low (2020)

London: Palgrave Macmillan, 323 pp.,

ISBN 978-1-34995-975-4, ebook, $69.99

ISBN 978-1-34995-974-7, h/bk, $89.99
Ride-along listening: Inclusive modes of musical analysis in Switched on Pop
Authors: Skjerseth Amy
Published: July 2022

Popular music and pop song-dissection podcasts often compete for top 40 listeners’ attention, but podcasts interject hosts’ opinions of songs that listeners may not share. This article introduces a phenomenon I call ‘ride-along listening’, where podcast hosts play isolated musical features to closely examine a song’s production and reception. Hosts’ instantaneous explanations of musical terms have the potential to make pop podcasts more inclusive for non-musically trained listeners. As I show, Switched on Pop’s Episode 80 dissects Janelle Monáe’s ‘Make Me Feel’ by playing the single’s harmonies and rhythms back-to-back with those of the blues, Michael Jackson and Prince. But guest host Lizzo – a classically trained flutist, songwriter, singer and rapper – especially makes Monáe’s social message of fluid sexuality palpable for specialist and non-specialist listeners alike. By foregrounding performing musicians’ embodied listening and knowledge, ride-along listening can provide inclusive ways of dissecting the medium and the message of pop music.
Lessons on popular music form, creation and reception through the Song Exploder podcast
Authors: Giuffre Liz
Published: July 2022

This article positions Hrishikesh Hirway’s Song Exploder as an archetypical example of how musical exploration, examination and education can come together in podcast form. Song Exploder’s combination of content and format allows audiences with a range of musical experience and interest to gain insight into how popular music is created at the level of individual songs, and more broadly in terms of genre and industry reception and delivery processes. Originally conceived as an audio-only podcast, versions of Song Exploder have also been staged ‘live in concert’, and in audio-visual format for Netflix. To demonstrate Song Exploder’s success, I situate it alongside similar music/media crossovers in print, radio and film and television, while also presenting findings from my five years experience of using Song Exploder as a teaching tool for undergraduate students in Australia.
New synergies between the podcast and music industries: Spotify plays the rhythm
Authors: Gallego J. Ignacio
Published: July 2022

The aim of this article is to analyse the synergies established between the podcast industry and the music industry (mainly labels and publishers). To this end, it examined the strategies of majors and indies in the development of specific series and services, the acquisition of production companies and the establishment of agreements with the large platforms and advertisers and developed a taxonomy of the content generated by these companies. These strategies have several objectives that will be studied from the political economy of cultural and media industries point of view: the promotion of artists, the exploitation of the historical catalogue in a transmedia development that puts songs into circulation generating income from reproduction and public communication rights and, of course, the opening of new businesses that generate profits from the two fundamental income channels: data and advertising. Consequently, the music companies managing audio objects and generating contents that compete in the new paradigm.
Student-Generated Teaching Materials
Authors: Ribosa Jesús , Duran David
Published: July 2022

Students can create products that take the form of instructional materials. A scoping review was carried out to map the research field of student-generated teaching materials, focusing on product types, information sources, learning-related matters, and researchers’ explanations. Based on 280 articles, four product types were identified: audio/visual materials, questions, texts, and educational games. Studies gathered information from product creation, product use, participants’ perceptions, and learning outcomes. Socio-cognitive and motivational learning-related matters for creators and users were reported concerning the subject matter, cross-curricular competencies, academic emotions, and engagement. In these studies, researchers interpreted their findings based on nine different explanations: active learning, audience effect, knowledge building, learning by teaching, motivational processes, peer learning, the role of ICT, scaffolding, and time-on-task and practice effect. Different lines for future research are discussed, related to the educational stages and knowledge areas, the research designs, and the relationship between research and practice.
Aspidistra yingjiangensis (Asparagaceae: Nolinoidae), a new species record for the flora of India
Authors: Kumar Roy Dilip , Rikertre Lytan , Nripemo Odyuo
Published: July 2022

Aspidistra yingjiangensis L. J. Peng (Asparagaceae: Nolinoidae) earlier known from Yunnan province in China is reported here as a new record for India from the state of Nagaland. A detailed taxonomic description along with photographs of the diagnostic characters is presented. Detailed comparison of the morphological characters of A. yingjiangensis with A. longifolia Hook. f., the only known Indian Aspidistra, has been given.
Development of a flexible pavement condition index for urban road network
Authors: Paz e Albuquerque Tairone , Almeida de Melo Ricardo , Bezerra de Morais Lucas Matheus , Quintino Lira Oliveira Lucas , Cirne de Azevedo Filho Azamor
Published: July 2022

In the urban pavement management, it is essential to develop urban pavement condition indexes to support Municipal Secretariat for Infrastructure on decisions making in relation to maintenance and rehabilitation (M&R). The aim was to develop an urban pavement condition index (UPCI) for flexible pavements and perform a comparative analysis with some pavement condition indexes (PCI). The work method followed such steps: definition of sampling size; formation of rating panel and training; data collection about present serviceability rating (PSR) and flexible pavement distress; development of an UPCI; and, comparative analysis with some PCI. Multilinear regression modeling based on distresses and PSR carried out the UPCI, in accordance with some statistical assumptions such as homoscedasticity, serial correlation, multicollinearity and normality tests. The PCI chosen carried out variability between them and the PSR, which was statistically analyzed by errors and correlation tests. Lastly, the proposition of an urban pavement condition index for flexible pavement allows a better M&R planning, budget distribution and benefits to the population, because well-kept pavements promote comfort, travel time reduction and safety to the users.
Ozanimod Maintenance Therapy After Cyclosporine Induction in Acute Severe Ulcerative Colitis
Authors: Cohen Nathaniel A. , Dalal Sushila R. , Choi David , Rubin David T.
Published: July 2022

Ozanimod has recently received US Food and Drug Administration approval for moderate-to-severe ulcerative colitis. Treatment of acute severe colitis remains a clinical challenge, and although many patients respond to cyclosporine therapy, there remains a relative paucity of maintenance options. This case report describes the first reported, successful use of ozanimod after hospitalization for acute severe ulcerative colitis and in tandem with cyclosporine. Although a longer follow-up is required, this report shows the feasibility of ozanimod in this clinical setting.
A Community-Based Pancreatic Cancer Screening Study in High-Risk Individuals: Preliminary Efficacy and Safety Results
Authors: Kandiah Jonathan , Lo Tammy , Jin Dugho , Melchior Landon , Krebs Thorsten L. , Anand Naveen , Ingram Susan , Krumholtz Pramila , Pandya Deep , Trinidad Antolin , Dong Xiang (Eric) , Seshadri Ramanathan , Bauman James , Lee Ronald , Frank Richard C.
Published: July 2022

INTRODUCTION:

Pancreatic cancer (PC) screening recommendations have been based on studies performed solely at high-volume academic centers. To make PC screening more widely available, community-based efforts are essential. We implemented a prospective PC screening study in the community of Fairfield County, CT, and report our early safety and efficacy results.

METHODS:

Eligible individuals were enrolled into an investigator-initiated study and underwent a baseline and 3 annual magnetic resonance imagings/magnetic resonance cholangiopancreatographies (MRIs/MRCPs) with gadolinium, biannual blood donations for biobanking, and assessments for anxiety and depression. All MRIs were presented at a multidisciplinary board to determine whether further investigation was warranted.

RESULTS:

Seventy-five individuals have been enrolled and 201 MRIs performed over a 2.6-year average length of follow-up. Abnormal pancreatic findings (predominantly small cysts) were detected in 58.7% of the participants. Among these, 6.7% underwent endoscopic ultrasound, with 1 case complicated by postprocedural pancreatitis. One surgical resection was performed on a 4.7-cm intraductal papillary mucinous neoplasm with a focus on low-grade pancreatic intraepithelial neoplasia. One incidental finding of fibrosing mediastinitis was detected. Anxiety and depression scores decreased over the course of this study from 21.4% to 5.4% and 10.7% to 3.6%, respectively.

DISCUSSION:

This preliminary report supports the feasibility of performing MRI/magnetic resonance cholangiopancreatographies-based PC screening as part of a clinical trial in a community setting. A longer follow-up is needed to better assess safety and efficacy. To the best of our knowledge, this is the first report from a community-based PC screening effort (clinicaltrials.gov ID: NCT03250078).
Activated Carbon from Coconut Shell: Synthesis and Its Commercial Applications-A Recent Review
Authors: Basha Nilufer Anwar , Rathinavel Thirumalaisamy , Sridharan Harini
Published: July 2022

Biomass is abundant in nature and can be utilized for the innovation of new materials. The selectivity of renewable energy resource for the production of activated carbon is quite challenging. This is because of the variance in the yield percentage of carbon. Our current review focuses on production of activated carbon obtained from coconut shell and its commercial applications in various fields. It gives complete structure of the different carbon materials that are activated in the form of a matrix, composite, deposition layer and graphene form. The significance of the bioresources is described as a starting material to produce AC to meet the challenges of the industrial and biomedical fields. The importance of parameters during carbon activation, such as carbonization temperature, selection of activating agents and the suitable method was discussed in detail. The importance of coconut shell as the best biomass producing highly efficient AC is compared with other raw materials. A deep insight into the study of raw materials essential for the preparation of activated carbon (AC) has been studied and reviewed thoroughly for the manifestation of efficient bioresource. Coconut shell is an underutilized renewable agricultural waste for production of AC via various synthesis protocols. This review explains the significance of coconut shell as a cheap substrate and eco-friendly material and the application of activated carbon for wastewater treatment, drug delivery, fabrication of energy storage devices and as an adsorbing agent.
Los desafíos éticos del transhumanismo
Authors: Cortina Adela
Published: July 2022

Sin duda la revolución 4.0 es ya el mundo en el que vivimos y proporciona instrumentos muy valiosos para construir una sociedad que pueda servir mejor a los seres humanos y al cuidado de la naturaleza desde las nuevas posibilidades. Pero para emprender ese camino es preciso optar por una ética que reconozca que lleva la intersubjetividad entrañada en su seno, porque es consciente de que no somos individuos aislados, sino que devenimos personas desde el reconocimiento recíproco como interlocutores válidos, como seres dotados de dignidad y no un simple precio, como quienes son carne de la misma carne y hueso del mismo hueso. Ésta sería una ética dialógica de la razón cordial.
Scientific-technological society, functional diversity and equal inclusion
Authors: Aparicio Payá Manuel
Published: July 2022

El objetivo de este trabajo, encuadrado en la perspectiva filosófica del entrecruzamiento entre los estudios sobre discapacidad (Disability studies) y los estudios filosófico-sociales sobre la ciencia y la tecnología (CTS), es abordar la cuestión de la accesibilidad universal, relacionada con el diseño para todas las personas. Pretendo llevar a cabo una reflexión ético-política sobre esta temática, en el contexto de las posibilidades abiertas por el desarrollo científico-tecnológico emergente y sus repercusiones en relación a los colectivos de personas con diferentes tipos de diversidad funcional.
La incertidumbre jurídica como respuesta del derecho a los dilemas del avance biotecnológico ¿paradoja o única solución posible?
Authors: Montalvo Jääskeläinen Federico de
Published: July 2022

En este complejo futuro que se nos predice, no solo se nos podrá superar en nuestras capacidades intelectuales o físicas, incorporando a nuestro entorno y a nuestro propio cuerpo un ingente aparataje tecnológico, sino que se nos podrá mejorar, y ello, incluso, antes de nacer, interviniendo directamente sobre el embrión. La naturaleza de lo humano se pone en cuestión, sobre todo, cuando la alteramos en su propia esencia, no solo en su entorno, y, además, desde su propio inicio. Se trata, pues, de una disrupción y alteración sustancial ad intra del ser humano, no ad extra, como ocurre con la tecnología, aún cuando esta también tiene, obviamente, muchos efectos en el interior de aquél. Los cambios que se derivan de la Biotecnología y, concretamente, de la edición genómica, por un lado, ofrecen un enorme potencial para desarrollar nuevos conceptos, métodos y novedosas aplicaciones para los urgentes retos y necesidades de la sociedad. Por el otro lado, tales biotecnologías son percibidas como si tuvieran el potencial de desafiar y, a veces, desdibujar las actitudes, valores y modelos culturales de la propia sociedad. A tal reto no puede estar, obviamente, ajeno el Derecho en atención a los valores, intereses y derechos en conflicto. Si la pregunta que debemos nuevamente formularnos es qué es el hombre o qué es la naturaleza humana, el Derecho debe también ofrecer respuestas que permitan dar un sentido jurídico contemporáneo a tal esencia, preguntas que conectan directamente con el propio concepto jurídico de dignidad, no solo en cuanto valor ontológico del ser humano, sino también, recogiendo los términos explícitos del artículo 10.1 de nuestra Constitución, en cuanto fundamento del orden político y de la paz social.
School choice, teachers’ work, and professional identity
Authors: Castro Andrene J. , Jabbar Huriya , Núñez Miranda Sebastian
Published: July 2022

Teacher professional identity, or what it means to be a teacher, informs the types of schools teachers seek for work. With the marketization of schools in the US and abroad, teachers’ professional identities are changing. However, we know little about how teachers negotiate—and renegotiate—their professional identities during the job search in contexts with school choice, such as charter schools. This study uses qualitative interview data from 46 teachers in San Antonio, Texas, where over 25% of students attend charter schools. Our findings illuminate the job search as a critical juncture where teachers evaluate their professional identity as they make choices about the sector—charter or traditional public school—and/or school organization they prefer. In particular, the choice context legitimated flexibility and fluidity in teachers’ professional identity as teachers moved between sectors to find jobs, even if the school did not align with their personal or professional values. We also found that employability and teachers’ perception of the job market played an important role in how teachers strategically presented their professional identity on the job search. Findings offer implications for teacher education and teacher workforce policies.
The emergence of a political-pedagogical teacher subjectivity: Chilean teachers’ struggle to bring pedagogy back into the political sphere
Authors: Acuña Felipe
Published: July 2022

This paper analyzes the emergence of a dissident subjective position among Chilean teachers as they struggle against a new Teachers Career Policy (TCP). Since the early 1980s, comprehensive neoliberal policies have reshaped Chilean society. During this period, teachers were described as an absent subject. However, in 2014 dissident teachers spontaneously asserted themselves against a new TCP inaugurating the teachers’ spring, which in 2015 involved a 57-days strike, and again 50 days in 2019. Using the Foucauldian notion of subjective limits, I present the results of a study based on a narrative approach to understand the formation of a dissident subjectivity among Chilean teachers. I conducted 35 interviews with ten leaders and eight grassroots teachers of seven different dissident teachers’ organizations. The findings focus on analyzing political-pedagogical dissent as the central discourse mobilized by teachers to disrupt the new TCP. This discourse enables dissident teachers to clarify the current limits of their work, allowing a set of experimental practices to unfold. Finally, I discuss the emergence of a dissident teacher’s subject as a case that evokes and illustrates how new forms of political subjectivities enable us to understand the current crisis of neoliberalism experienced in Chile.
Diagnostic accuracy of calf circumference for decreased muscle mass in older adults with sarcopenia
Authors: Pusparini Novi Diah , Probosari Enny , Murbawani Etisa Adi , Muis Siti Fatimah , Christianto Febe
Published: July 2022

Background: Increasing number of the older adults population results in increasing sarcopenia, a geriatric problem that may lead to poor quality of life, susceptibility to disease, malnutrition, and even death. Muscle mass is an important sarcopenia parameter that can be measured by Bioelectrical Impedance Analysis (BIA). Detection of decreased muscle mass can be done by measuring calf circumference, it is expected to provide an early diagnose of sarcopenia so that early intervention can be given and improve the quality of life of the older adults.Objective: To analyze the diagnostic accuracy of calf circumference for decreased muscle mass in older adults to provide simple way in diagnosing sarcopenia.Methods: This study involved 126 older adults, consisted of 57 men and 69 women aged 60-80 years in the community who met the inclusion criteria. Criteria of sarcopenia were defined based on the Asian Working Group for Sarcopenia (AWGS) Consensus, consisted of three components; muscle mass, handgrip strength, and walking speed. This study analyze the diagnostic accuracy of calf circumference for decreased muscle mass measured by single- frequency BIA and calf circumference was measured using a measuring tape. The analysis was carried out according to the receiver operating characteristic (ROC) curve to determine the cut-off point along with the sensitivity (Se) and specificity (Sp) values, positive and negative predictive values (PPV and NPV) of calf circumference as an indicator for low muscle mass.Results: Optimal cut-off point of calf circumference to indicate low muscle mass is 32.9 cm in women (Se 80.8%, Sp 79.1%, PPV 75.9%, NPV 87.5%) and 33.5 cm in men (Se 78.6%, Sp 74.4%, PPV 50%, NPV 91.4%). PPV in men is lower than women. This is due to a lower prevalence of decreased muscle mass in men than women. There were 49 participants with the calf circumference below cut-off point and 40 (31.7%) of the 126 participants had sarcopenia.Conclusion: Calf circumference has a diagnostic accuracy to find decreased muscle mass in  sarcopenia.
Dilemma of presymptomatic testing in children with history of late onset neurodegenerative spinocerebellar ataxia in Indonesia
Authors: Sihombing Nydia Rena Benita , Winarni Tri Indah , Belladonna Maria , Ediati Annastasia , Faradz Sultana MH
Published: July 2022

Background: Spinocerebellar ataxia (SCA) is a late onset neurodegenerative disorder in which coordination and balance are affected. Although many international guidelines have been established regarding presymptomatic testing, it is still a grey area in Indonesia. We report two large families with advanced stages of SCA who underwent presymptomatic genetic testing in children along counseling process.Case presentation: Thorough examination was performed, including pedigree construction, physical and neurological examination, gene mutation analyses for patients, and presymptomatic testing for family members, including children. SCA3/MJD1 gene mutation analysis was done in both cases, and a full penetrance CAG repeat expansion was found in both affected patients. Two different outcomes were observed in the offspring, who were both children. The risk and consequences of positive results had been explained in a counseling session to family members, who decided to keep the information until the child would have reached legally adult age.Conclusions: In developing countries such as Indonesia, problems arose due to ethical issues, knowledge of genetic diseases, and inaccessible molecular diagnostics. Culture, religion and tribe diversities may create additional challenges. These cases emphasize the need for careful consideration of presymptomatic testing in children, especially in complicated situations where psychological and ethical issues should be addressed.
Appraising Students’ Attitude towards Online Learning During Covid-19 Pandemic
Authors: DINAMIKA SORAYA GRABIELLA
Published: July 2022

This study aimed at evaluating the students’ attitude toward online learning during the Covid-19 pandemic within the written discourse by using the Appraisal Theory arranged by Martin & White, under the region of appreciation. Appreciation dealt with the evaluation of semiotic and natural phenomena. The realization of appraising item was adjectival that might have either positive or negative meaning. The data were obtained from the essays written by 25 students who joined the conversation subject through the online classroom. This study engaged with the qualitative descriptive method. The objectives of this study were three extents: (a) to find out the most predominant appreciation items the students tried to convey, (b) to find out the students’ value position, and (c) to give the pedagogical suggestion on the online learning. The result of the analysis showed that within 137 appraising items found, the positive appraising item exceeded the account of 52,5%. It reflected that the students had positive attitude toward online learning activities. Moreover, the students presented their value position as agreeing with the activity of online learning. Therefore, constructive pedagogical suggestions were given to the lecturers, the students, and the institution stakeholders.
Actualizing Learning English Grammar Strategy Using New Inventory for University Students
Authors: Syafryadin Syafryadin
Published: July 2022

Learning grammar strategies have been explored by students and teachers. Moreover, research on this issue has been done by several researchers. However, new inventory to measure learning English grammar strategy has been developed and not implemented to identify students’ learning strategy. Thus, the present study aimed to implement and identify the students’ English learning grammar strategy by using new inventory. Besides, this study also wanted to know the challenges of students in implementing those strategies. This study used Mixed method with sequential explanatory.  The participants of this research were 30 students of English Language Department in one of the universities in Bengkulu. The instrument of this study used questionnaire of Grammar Learning Strategy Inventory (GLSI) and the interview guideline.  Those instruments have been validated by two expert judgments and tried out to another class. The quantitative data were analysed by using simple statistic calculation and using percentage for every learning strategy type. However, the qualitative data analysis comprised several steps namely data collection, data condensation, data display, and conclusion. The result shows that students used all strategies in learning grammar, but the dominant one is cognitive strategy, while the least one is social strategy.   The challenges in implementing learning strategy or in learning grammar were poor knowledge of grammar, ineffective time management, less group work, and less sociable because of Covid-19.
PERANCANGAN DAN PENGENDALIAN MANIPULATOR ROBOT 4-DOF DENGAN GRIPPER BERBASIS INVERSE KINEMATICS DAN TRAJECTORY PLANNING DENGAN ROS
Authors: Setiyadi Ahmad Didik , Setiawan Iwan , Afrisal Hadha
Published: July 2022

Penyebaran virus korona terjadi dari manusia ke manusia melalui droplet dan udara, kontak fisik atau jarak dekat manusia ke manusia merupakan penyebab utama penularan virus korona meskipun dengan penggunaan alat perlindungan diri (APD), ini dibutktikan dengan meninggalnya lebih dari 1400 tenaga medis karena terpapar COVID-19 per tanggal Juli 2021 di Indonesia.  Penelitian ini dirancang robot manipulator 4 DOF (RPRR) dengan sistem ROS menggunakan penggerak motor stepper Nema 17 serta dengan sistem pengendalian inverse kinematics dan trajectory planning dengan fungsi sigmoid yang bekerja pada joint space. Untuk mengurangi osilasi pada pergerakan robot ditambah kontrol proporsional dengan nilai KP=10 yang dihasilkan dengan metode trial and error. Robot dirancang untuk memindahkan tabung reaksi. Dari hasil rancangan robot yang dibangun robot dapat melakukan pergerakan dengan presisi ditunjukan dengan pengujian kinematika balik dengan galat rata-rata pada sumbu x sebesar 0,62 mm, pada sumbu y sebesar 0,55 mm, dan pada sumbu z sebesar 0,36 mm. Performa dari trajectory planning pada joint space saat mencapai koordinat tujuan joint 1 memiliki galat 0,20º, joint 2 sebesar 0,20º, pada joint 3 sebesar 0,17º, dan pada joint 4 sebesar 0.18º. Robot manipulator 4 DOF saat melakukan tugas pick and place tabung reaksi memiliki galat rata-rata 1,46 mm.
PERANCANGAN DAN PENGENDALIAN DIFFERENTIAL DRIVE ROBOT DENGAN MENGAPLIKASIKAN METODE A-STAR
Authors: Nugraha Ghanis Kauchya , Setiawan Iwan , Afrisal Hadha
Published: July 2022

Pandemi COVID-19 menyerang seluruh penduduk dunia. Pandemi COVID-19 juga menyebabkan banyaknya kematian tenaga medis di Indonesia dan dunia. Solusi yang dapat diterapkan untuk mengurangi jumlah tenaga medis yang terpapar virus SARS-CoV-2 adalah dengan menerapkan robot yang dapat membantu tenaga medis dalam bekerja. Robot harus bisa bergerak dari tempat satu ke tempat yang lain dan bisa membawa perlengkapan yang diperlukan seperti obat-obatan, alat medis, dll. Robot differential drive menggunakan 2 roda yang digerakkan oleh penggerak terpisah sebagai penggerak utama. Mobile robot membutuhkan path planning yang optimal dalam melakukan navigasi dari suatu koordinat ke koordinat lain. Dalam tugas akhir ini penulis merancang sistem navigasi robot tipe differential drive dengan menerapkan algoritma A*. Hasil pengujian menunjukkan data nilai galat maksimum adalah 0.073 meter atau 7.3 cm terjadi pada detik 138.8. Nilai rata-rata galat absolut robot dalam bernavigasi sebesar 0.017 meter atau sebesar 1.7 cm. Robot bernavigasi dengan kecepatan linear rata-rata 0.23 m/s. Waktu yang dibutuhkan program untuk menghasilkan rencana rute adalah 422.99 ms. Waktu yang dibutuhkan robot untuk melakukan navigasi dari titik awal ke titik target adalah 170.16 detik.
ANALISIS EKONOMI DAN PENGUJIAN PEMBANGKIT LISTRIK TENAGA SURYA SISTEM OFF GRID PADA UMKM COFFESHOP DI KOTA SEMARANG
Authors: Masfuha Sunan Muqtasida , Windarta Jaka , Handoko Susatyo
Published: July 2022

Distribusi listrik dari Perusahaan Listrik Negara (PLN) belum tentu mencapai daerah daerah terpencil, sehingga daerah tersebut memerlukan sumber listrik alternatif seperti pembangkit listrik tenaga surya (PLTS). Maka dari itu dalam tugas akhir ini akan dilakukan penelitian yang ditujukan untuk menganalisis perancangan PLTS dengan sistem off grid ditinjau dari sisi ekonomi teknik dan menguji sistem PLTS tersebut pada coffee shop di remote area. Metode penelitian yang digunakan adalah membandingkan kelayakan 4 variasi sistem PLTS dengan sistem genset dan  sistem charging. Didapatkan hasil penelitian semua variasi memiliki nilai NPV yang bernilai negatif (<0). Dengan demikian semua variasi ini dianggap tidak layak dari segi ekonomi dan tidak memberikan profit, akan tetapi pilihan PLTS variasi 1 adalah investasi yang paling murah. Dengan alasan tersebut PLTS variasi 1 yang menggunakan panel surya Maysun 120 Wp dan baterai Shoto 150 Ah dipilih sebagai sistem yang dilakukan pengujian. Dari hasil pengujian yang dilakukan, didapatkan semua komponen dan sistem secara keseluruhan dapat bekerja dengan baik. Hal ini dapat disimpulkan bahwa sistem PLTS layak untuk diimplementasikan.
ANALISIS POTENSI DAN UNJUK KERJA PERENCANAAN PEMBANGKIT LISTRIK TENAGA SURYA DI GEDUNG FAKULTAS PSIKOLOGI UNIVERSITAS DIPONEGORO
Authors: Nugroho Widiyanto , Nugroho Agung , Winardi Bambang
Published: July 2022

Indonesia merupakan negara yang secara geografis dilalui oleh garis khatulistiwa sehingga memiliki potensi besar dalam hal pemanfaatan energi matahari. Hal ini dikarenakan besarnya radiasi matahari dipengaruhi oleh letak garis lintang, kondisi atmosfer, dan posisi matahari terhadap garis khatulistiwa. Menurut Power Data Acces Viewer NASA pada tahun 2019 Semarang memiliki tingkat radiasi rata-rata yang relatif tinggi yaitu sebesar 5,5 kWh/m2/hari. Hal ini adalah sebuah keuntungan besar dalam hal pemanfaatan energi matahari menjadi energi listrik melalui Fotovoltaik. Fakultas Psikologi Universitas Diponegoro memiliki gedung yang mengusung konsep Undip First Bioclimatic and Biophilic Green Building. Gedung ini memiliki sebuah sarana parkir sepeda motor dan sebuah rooftop terbuka yang belum teroptimalkan dengan baik.  Dengan intensitas radiasi matahari yang cukup tinggi tiap harinya, gedung ini dapat memanfaatkan rooftop dan sarana parkir sepeda motornya menjadi siteplan PLTS yang cukup besar. PLTS On Grid merupakan sistem PLTS yang terintegrasi dengan jaringan jala-jala PLN, di mana PLN sebagai pemasok energi utama dan PLTS sebagai pemasok energi tambahan. Melalui software PVSyst 6.43, potensi dan kinerja dari perencanaan PLTS di Fakultas Psikologi Universitas Diponegoro ini diperkirakan memiliki potensi 387,7 MWh tiap tahunnya. Setelah melalui proses konversi energi listrik berkurang menjadi 374,11 MWh dengan  pembagian 260,05 MWh digunakan untuk mensuplai beban dan 114,06 MWh dikirim ke jaringan jala-jala listrik PLN (Grid).
Multiple mechanisms underlie reduced potassium conductance in the p.T1019PfsX38 variant of hERG
Authors: Al Salmani Majid K. , Tavakoli Rezvan , Zaman Wajid , Al Harrasi Ahmed
Published: July 2022

Long QT syndrome type II (LQT2) is caused by loss‐of‐function mutations in the hERG K+ channel, leading to increased incidence of cardiac arrest and sudden death. Many genetic variants have been reported in the hERG gene with various consequences on channel expression, permeation, and gating. Only a small number of LQT2 causing variants has been characterized to define the underlying pathophysiological causes of the disease. We sought to determine the characteristics of the frameshift variant p.Thr1019ProfsX38 (T1019PfsX38) which affects the C‐terminus of the protein. This mutation was identified in an extended Omani family of LQT2. It replaces the last 140 amino acids of hERG with 37 unique amino acids. T1019 is positioned at a distinguished region of the C‐terminal tail of hERG, as predicted from the deep learning system AlphaFold v2.0. We employed the whole‐cell configuration of the patch‐clamp technique to study wild‐type and mutant channels that were transiently expressed in human embryonic kidney 293 (HEK293) cells. Depolarizing voltages elicited slowly deactivating tail currents that appeared upon repolarization of cells that express either wild‐type‐ or T1019PfsX38‐hERG. There were no differences in the voltage and time dependencies of activation between the two variants. However, the rates of hERG channel deactivation at hyperpolarizing potentials were accelerated by T1019PfsX38. In addition, the voltage dependence of inactivation of T1019PfsX38‐hERG was shifted by 20 mV in the negative direction when compared with wild‐type hERG. The rates of channel inactivation were increased in the mutant channel variant. Next, we employed a step‐ramp protocol to mimic membrane repolarization by the cardiac action potential. The amplitudes of outward currents and their integrals were reduced in the mutant variant when compared with the wild‐type variant during repolarization. Thus, changes in the gating dynamics of hERG by the T1019PfsX38 variant contribute to the pathology seen in affected LQT2 patients.
Bounded solutions to systems of fractional discrete equations
Authors: Diblík Josef
Published: July 2022

The article is concerned with systems of fractional discrete equations

Δ

α

x

(

n
+
1

)

=

F

n

(

n
,
x

(

n

)

,
x

(

n
−
1

)

,

…

,
x

(

n

0

)

)

,

n
=

n

0

,

n

0

+
1
,

…

,

{\Delta }^{\alpha }x\left(n+1)={F}_{n}\left(n,x\left(n),x\left(n-1),\ldots ,x\left({n}_{0})),\hspace{1em}n={n}_{0},{n}_{0}+1,\ldots ,

where

n

0

∈
Z

{n}_{0}\in {\mathbb{Z}}

,

n

n

is an independent variable,

Δ

α

{\Delta }^{\alpha }

is an

α

\alpha

-order fractional difference,

α
∈
R

\alpha \in {\mathbb{R}}

,

F

n

:

{

n

}

×

R

n
−

n

0

+
1

→

R

s

{F}_{n}:\left\{n\right\}\times {{\mathbb{R}}}^{n-{n}_{0}+1}\to {{\mathbb{R}}}^{s}

,

s
⩾
1

s\geqslant 1

is a fixed integer, and

x
:

{

n

0

,

n

0

+
1
,

…

}

→

R

s

x:\left\{{n}_{0},{n}_{0}+1,\ldots \right\}\to {{\mathbb{R}}}^{s}

is a dependent (unknown) variable. A retract principle is used to prove the existence of solutions with graphs remaining in a given domain for every

n
⩾

n

0

n\geqslant {n}_{0}

, which then serves as a basis for further proving the existence of bounded solutions to a linear nonhomogeneous system of discrete equations

Δ

α

x

(

n
+
1

)

=
A

(

n

)

x

(

n

)

+
δ

(

n

)

,

n
=

n

0

,

n

0

+
1
,

…

,

{\Delta }^{\alpha }x\left(n+1)=A\left(n)x\left(n)+\delta \left(n),\hspace{1em}n={n}_{0},{n}_{0}+1,\ldots ,

where

A

(

n

)

A\left(n)

is a square matrix and

δ

(

n

)

\delta \left(n)

is a vector function. Illustrative examples accompany the statements derived, possible generalizations are discussed, and open problems for future research are formulated as well.
Phenology of Codium cylindricum (Ulvophyceae, Bryopsidales) on the central Pacific coast of Japan
Authors: Miyadai Minoru , Akita Shingo , Fujita Daisuke
Published: July 2022

Codium cylindricum is a large green alga distributed along the temperate Northeast Pacific coasts. Although the species is edible and contains pharmaceutical substances, little is known about its ecology. In this study, we characterized the phenology of C. cylindricum by monthly observations in Tateyama on the central Pacific coast of Japan from March 2018 to February 2019. The average length of attached thalli in three quadrats (2 × 2 m) was 5 cm from April to July. After disappearing in August, recruits appeared in November and the maximum density (1.08 ± 3.5 inds·m−2) was reached in February. On the sea bottom, detached thalli were present from April to October; the biomass monitored in a single quadrat (2 × 20 m) was high from June to August. Based on monthly observations of 50–100 utricles in each of 18–25 thalli, gametangium formation started in June and reached a peak (i.e., gametangia detected in 75% of thalli and 63.4% of utricles) in September, before the disappearance of the detached thalli. These results strongly suggest that C. cylindricum is annual and reproduces mainly while they are drifting. Therefore, growth during detached stage is critically important to increase biomass and dispersal.
Cephalic shield morphology as species diagnostic trait and individual natural mark in three-banded armadillos (Tolypeutes; Cingulata: Chlamyphoridae)
Authors: Magalhães Rodolfo Assis , Feijó Anderson , Bocchiglieri Adriana , de Sena Liana Mara Mendes , Attias Nina , Rodrigues Flávio Henrique Guimarães
Published: July 2022

Previous studies have suggested species- and individual-specific morphologies of cephalic shields of three-banded armadillos (Tolypeutes spp.). Central cephalic scutes would form discrete lines; the first two posterior lines being composed of one scute (1-1) in Tolypeutes matacus and one followed by two scutes (1-2) in Tolypeutes tricinctus. However, conclusions were limited to a few specimens and the T. tricinctus pattern was later observed in T. matacus. Here, we assessed the potential use of the morphology of the cephalic shield as a species diagnostic trait and individual natural mark in three-banded armadillos by quantifying their patterns in museum (n = 71) and field (n = 82) specimens. In total, 93% of T. matacus and 88% of T. tricinctus specimens presented the respective species’ character states. All specimens could be individualized based on a visual comparison of their cephalic shields. Our findings show that the morphology of cephalic shields is a species diagnostic trait and an individual natural mark in three-banded armadillos. Therefore, we add an accessible character for species diagnosis in Tolypeutes and provide an alternative to artificial marks that presents virtually no associated cost and damage to animal welfare and can be reliably applied in ecological and participatory science projects.
Expanding dual-task research by a triple-task
Authors: Stefani Maximilian , Sauter Marian , Eichert Franziska , Mack Wolfgang
Published: July 2022

Multitasking research in the laboratory is dominated by extremely simplistic dual-task paradigms. Although dual-tasks allow for some variations, they do not compare well to more complex everyday task settings. This study expands a classical dual-task paradigm by adding a third task. The dual-tasks and the triple-task always consisted of the same three single tasks. The aim was to investigate the effects of the combinations of the three single-tasks and in which manner response times and costs increased. Stimulus-response pairings were varied either once within participants (E1) or between participants (E2). Our results showed that the increase in response time from dual-tasks to triple-tasks was only 43% of the increase from single-tasks to dual-tasks suggesting a non-linear cost of adding tasks. Moreover, response times in each subtask were higher in triple-task situations compared to single-task or dual-task situations. This is in contrast to classical dual-tasks, in which typically only one of the two responses is delayed. Cognitively, for costs in triple-tasks, unlike in dual-tasks, task coordination seems to play a larger role compared to the classically suggested relationships between stimulus and response in terms of their modality- and ideomotor-compatibility which we will discuss. Overall, the study demonstrates that current multitasking research is limited in its generalizability by focusing only on dual-tasks and would benefit from research with more complex task settings.