bluuebunny/crossref_metadata_2025 huggingface dataset:
Policing, Punishment, and Place: Spatial-Contextual Analyses of the Criminal Legal System
Authors: Simes Jessica T. , Beck Brenden , Eason John M.
Published: March 2023

Policing and punishment are unevenly distributed across geographic space. Research analyzing place-based variation in the criminal legal system is increasing, asking how community conditions contribute to variation in criminal justice outcomes and how multiple criminal justice exposures (e.g., policing and punishment) vary together in places. In this article, we identify spatial-contextual analyses of the criminal legal system and summarize their contributions by organizing them by their three major approaches: those emphasizing crime, urban ecology, or social control. We describe challenges the subfield faces, including an overemphasis on large cities and an overcommitment to analyzing criminal justice institutions like police or prisons discretely, when they are often experienced cumulatively and simultaneously. We call for research that transcends received institutional divisions, generates recommendations for stakeholders at multiple scales, makes greater use of formal spatial modeling, and analyzes places across the urban-rural continuum.
Fe(II)-Based Metallo-Supramolecular Polymer Film for Electrochemical Detection of Nitrite: Studies of Kinetics and Reaction Mechanisms
Authors: Awal Abdul , Mia Md. Mithu , Sarkar Shifa , Islam Santa , Sarker Sohag , Nayem S. M. Abu , Hossain Md. Delwar , Ahammad A. J. Saleh
Published: March 2023

Here, a monometallic supramolecular polymer (SMP) was synthesized for the fabrication of an electrochemical nitrite sensor, and a mechanism for nitrite detection was proposed based on the experimental findings. The SMP (polyFe) was synthesized using a symmetrical ligand containing terpyridine moieties [4′,4′′′′-(1,4- Phenylene) bis(2,2′:6′,2′′-terpyridine)] and ferrous acetate. Various analytical methods, such as ultraviolet/visible (UV/Vis) titration, field-emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDS), were used to characterize polyFe. The molecular weight of polyFe was calculated from the intrinsic viscosity measurement using the Mark-Houwink-Sakurada equation. The electrochemical behavior of the fabricated sensor was investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The variation in scan rate from CV was used to investigate the kinetics of nitrite oxidation. A possible reaction mechanism was proposed based on the kinetic studies. The proposed sensor showed a good linear range of 2.49 μM to 1.23 mM and a limit of detection of 0.17 μM. Stability, interference, and reproducibility of the proposed sensor were also investigated. The CV technique was used to demonstrate the applicability of the nitrite sensor for real sample analysis. A satisfactory recovery with a low relative standard deviation was achieved.
Organelle Communication: Joined in Sickness and in Health
Authors: Bravo-Sagua Roberto , Lopez-Crisosto Camila , Criollo Alfredo , Inagi Reiko , Lavandero Sergio
Published: March 2023

Organelles are membrane-lined structures that compartmentalize subcellular biochemical functions. Therefore, interorganelle communication is crucial for cellular responses that require the coordination of such functions. Multiple principles govern interorganelle interactions, which arise from the complex nature of organelles: position, multilingualism, continuity, heterogeneity, proximity, and bidirectionality, among others. Given their importance, alterations in organelle communication have been linked to many diseases. Among the different types of contacts, endoplasmic reticulum mitochondria interactions are the best known; however, mounting evidence indicates that other organelles also have something to say in the pathophysiological conversation.
Denoising Method of Pipeline Leakage Signal Based on VMD and Hilbert Transform
Authors: Jiang Zhu , Xie Junyi , Zhang Jing , Zhang Xiang
Published: March 2023

In order to solve the problem of low localization accuracy of water supply pipeline leakage detection using acoustic emission signals, a noise reduction algorithm based on variational mode decomposition (VMD) and Hilbert transform is proposed in this paper. Firstly, the leakage signal is decomposed into several intrinsic mode functions (IMF) using VMD, and the number of decomposition layers is determined, and the IMF component is transformed by Hilbert to get the marginal spectrum, and the noise are preliminarily filtered according to the marginal spectrum characteristics and the cross‐correlation coefficient. Secondly, the signal denoising is realized by using filtering technology and cross‐correlation coefficient. Finally, the time delay estimation of the denoised signal is estimated and the location of the leakage point is calculated based on the positioning principle. In order to verify the performance of the algorithm, a water supply pipeline experiment platform for leakage simulation and leak location experiment was conducted. The cross‐correlation algorithm, VMD‐correlation coefficient algorithm, and the proposed algorithm are analyzed through simulation and experiment. The results show that compared with the other two algorithms, the proposed algorithm can effectively remove the noise in the leakage signal and improve the accuracy of leakage location.
Research on Productivity Evaluation Method Based on Shale Gas Well Early Flow Stage Data
Authors: Huang Yize , Li Xizhe , Guo Wei , Liu Xiaohua , Lin Wei , Qian Chao , Zhou Mengfei , Cui Yue , Shi Xiaomin
Published: March 2023

Since the accurate early gas well production regime is related to the production period and final productivity, it is crucial to make better use of early flow stage production data for gas well productivity evaluation. Absolute open flow potential (AOF) and estimated ultimate recovery (EUR) are essential parameters for evaluating the productivity of shale gas wells. This study establishes new AOF calculation methods for the early production stage. The analytical model can calculate the AOF only by using stable pressure and production data in the flowback stage, which greatly improves the efficiency of productivity evaluation. Three methods have, respectively, calculated the productivity of the shale gas wells above 3500 m in the Luzhou block. The results show that well L2-3 has the highest AOF, averaging

278.1
×

10

4

 

m

3

/
d

, whereas well Y2-8 has the lowest AOF, averaging

100.2
×

10

4

 

m

3
/

d

. Different AOF calculation methods are identified for gas wells in different stages of production. For gas wells in the initial unstable flow stage, a pseudogas production index method is recommended. A water production index analysis method, with lower evaluation results, is proposed for gas wells in the flowback stage. A modern production decline analysis method is found to be preferred for calculating the EUR of deep shale gas wells. Well L2-3 has the highest average EUR of

1.26
×

10

8

 

m

3

, whereas well Y2-8 has the lowest average EUR of

0.42
×

10

8

 

m

3

. The Blasingame method is recommended for medium-to-high-production gas wells, whereas a normalized pressure integral method is suggested for low-production wells. A strong exponential quantitative link between the AOF and the EUR shows that a fracture system’s initial productivity has a significant impact on a well’s EUR. The findings of this study enrich the productivity evaluation system, increase the accuracy of productivity evaluation results, and provide theoretical support for deep shale gas wells.
Ultrasound-Guided Rhomboid Block versus Paravertebral Block in Postoperative Analgesia for Video-Assisted Thoracoscopic Surgery: A Prospective Randomized Controlled Clinical Trial
Authors: Wang Yan , Gu Xiaoping , Huang Simin , Shi Minke , He Xiaofeng , Ma Zhengliang
Published: March 2023

Introduction. The anesthetic efficacy of the ultrasound-guided rhomboid intercostal block (RIB) in alleviating postoperative pain has been well concerned. This study aims to compare the effectiveness between ultrasound-guided RIB and paravertebral block (PVB) in alleviating acute pain following video-assisted thoracic surgery. Methods. It was a prospective, randomized, double-blinded clinical trial involving 132 patients with video-assisted thoracic surgery divided into three groups: the general anesthesia (GA) group, RIB group, and PVB group on T5 vertebra, using 0.4% ropivacaine at 3 mg/kg, registered in the Chinese Clinical Trial Registry (ChiCTR2100054057, “https://www.chictr.org.cn”). The visual analogue scale (VAS) scores at rest and cough during 48 h postoperatively and the postoperative consumption of pain rescue were the primary outcomes, and the QoR15 score 48 h postoperatively, the usage of opioids during and after operation, and nerve block-related complications were the secondary outcomes. Demographic characteristics, surgery characteristics, and primary outcomes between the groups were compared. Results. A total of 120 eligible patients were recruited, including 40 in each group. Baseline and surgery characteristics between the groups were comparable (all

p
>
0.05

). The PVB and RIB groups were better than the GA group in the primary and secondary outcomes (

p
<
0.05

). The static VAS score, QoR15 score, and block-related complications within 48 hours after surgery were better in the RIB group than in the PVB group (

p
<
0.001

). Conclusion. Both PVB and RIB can provide adequate analgesia and accelerate the recovery of patients. Compared with PVB, RIB has a better analgesic effect, especially to avoid paravertebral pain caused by block, and the operation of RIB is more straightforward and the safety is higher.
A Systematic and Comprehensive Survey of Recent Advances in Intrusion Detection Systems Using Machine Learning: Deep Learning, Datasets, and Attack Taxonomy
Authors: Momand Asadullah , Jan Sana Ullah , Ramzan Naeem
Published: March 2023

Recently, intrusion detection systems (IDS) have become an essential part of most organisations’ security architecture due to the rise in frequency and severity of network attacks. To identify a security breach, the target machine or network must be watched and analysed for signs of an intrusion. It is defined as efforts to compromise the confidentiality, integrity, or availability of a computer or network or to circumvent its security mechanisms. Several IDS have been proposed in the literature to efficiently detect such attempts exploiting different characteristics of cyberattacks. These systems can provide with timely sensing the network intrusions and, subsequently, notifying the manager or the responsible person in an organisation. Important actions are then carried out to reduce the degree of damage caused by the intrusion. Organisations use such techniques to defend their systems from the network disconnectivity and increase reliance on the information systems by employing intrusion detection. This paper presents a detailed summary of recent advances in IDS from the literature. Nevertheless, a review of future research directions for detecting malicious operations and launching different attacks on systems is discussed and highlighted. Furthermore, this study presents detailed description of well‐known publicly available datasets and a variety of strategies developed for dealing with intrusions.
Young Consumers’ Usability Preferences for Mobile and Computer Screens for Online Shopping Activities
Authors: Khan Shahid Kalim , Khan Naseer Abbas , Quratulain  , Egorova Olga , Zia Nimra , Akhtar Maria
Published: March 2023

The current research endeavors to investigate and contrast consumer usability preferences in the context of electronic commerce (e-commerce) and mobile commerce (m-commerce) using the theoretical framework of the theory of planned behavior (TPB). In order to distinguish the variations in usability preferences between e-commerce and m-commerce, a survey consisting of 37 items was administered to a sample of 213 young Chinese respondents. The survey is aimed at gathering information pertaining to the usability aspects of these two online shopping platforms. The findings of this research indicate that consumer preferences for m-commerce and e-commerce are contingent on the specific context of online shopping. The results suggest that individuals tend to prefer one medium over the other based on the specific situation or task at hand. This information is valuable for professionals in the fields of marketing, branding, and distribution as it can inform the development of effective strategies for optimizing online presence and revenue. Specifically, knowledge of consumer preferences can assist in the creation of appropriate communication, branding, and distribution strategies tailored to the specific contexts in which consumers are likely to engage in e-commerce and m-commerce.
Determination of the Selected Heavy Metal Content and Its Associated Health Risks in Selected Vegetables Marketed in Bahir Dar Town, Northwest Ethiopia
Authors: Asrade Biset , Ketema Gebremariam
Published: March 2023

Introduction. Fresh vegetables are of a great value and the most common foods in the human diet around the world. They contain minerals, vitamins, antioxidants, antimetabolites, protein, carbohydrates, and water. However, they are a major source of heavy metals, which contain both essential and toxic heavy metals over a wide range of concentrations. The study sites serve as a regional commercial center, where vegetables such as cabbages, onions, carrots, potatoes, beetroots, lettuce, and tomatoes were irrigated year round and sold to consumers. The aim of the study was to determine the level of cadmium, chromium, copper, and lead and to estimate the health risks associated with their daily intake of vegetables marketed in Bahir Dar town, Northwest Ethiopia. Methods. The concentrations of copper (Cu), chromium (Cr), cadmium (Cd), and lead (Pb) were determined, and their health risks were estimated using estimated daily intake (EDI), target hazard quotient (THQ), hazard index (HI), and target cancer risk (TCR) for selected heavy metals by using flame atomic absorption spectrometry. A total of 5 kg composite samples for each type of vegetable were collected by simple random sampling from an open market in Bahir Dar town, Northwest Ethiopia, and subsamples (1.25 kg) were digested via the wet digestion method. Results. The average concentrations of Cr and Cu in all selected vegetables were lower than the maximum limit of expected values. However, the concentration (mg.kg−1) of Pb in carrots, potatoes, and beetroots exceeded the safe limit of 0.3 mg·kg−1 set by the FAO/WHO and the concentration of Cd (mg·kg−1) in carrots, which is higher than the FAO/WHO limit, 0.2 mg·kg−1, in dry weight. The health index (HI) values (mg·kg−1 day−1) of all vegetables except carrots were lower than one. The TCR values for Pb, Cd, and Cr in all vegetables exceeded the US-EPA recommended threshold risk limit, but the TCR value of carrot was above the moderate risk limit (>1 × 10−3) set by the US-EPA. Conclusion. This study indicated that the concentration level of lead and cadmium in potatoes, onions, carrots, and beetroots marketed in Bahir Dar town is not safe for use by the local community. The consumption of carrots in the study area may cause both noncancerous and cancerous health risks. Therefore, regular monitoring of these toxic metals in vegetables should be carried out to prevent heavy metal toxicity associated with the consumption of those vegetables marketed in Bahir Dar town, Ethiopia.
Use of Continuous Intravenous Anakinra Infusion in Multisystem Inflammatory Syndrome in Children
Authors: Alolayan Abdulaziz , Aldamegh Abdullah S. , Alkhayrat Azzah
Published: March 2023

Coronavirus disease (COVID-19) is an emergency pandemic with a high mortality rate worldwide. One of its complications in children is developing multisystemic inflammatory syndrome related to cytokine storm. Anakinra is a recombinant human interleukin-1 (IL-1) receptor antagonist used to suppress the exaggerated inflammatory response in such conditions, and it is potentially lifesaving in a cytokine storm. We present the case of a patient with critical COVID-19 associated with multisystem inflammatory syndrome in children (MIS-C) successfully treated with anakinra intravenous (IV) infusion.
Outcomes of Microdissection Testicular Sperm Extraction/Intracytoplasmic Sperm Injection in Cases of Nonobstructive Azoospermia: A Retrospective Study
Authors: Sun Xiaoming , Zhu Haibo , Yang Xiao , Wang Ruixue , Liu Ruizhi , Luo Lili
Published: March 2023

The outcomes and safety of intracytoplasmic sperm injection (ICSI) using testicular sperm have been controversial. We evaluated ICSI results, pregnancy outcomes, and neonatal health conditions using testicular sperm from patients with obstructive (OA) or nonobstructive (NOA) azoospermia. We further compared the ICSI outcomes after use of fresh versus cryopreserved testicular sperm from men with NOA. We included 314 men with NOA who underwent microdissection testicular sperm extraction (mTESE) and 303 with OA who underwent testicular sperm aspiration; both groups underwent ICSI. Therefore, 101 and 329 ICSI cycles were performed for mTESE and aspirated sperms, respectively. Furthermore, fresh and thawed embryos from both groups were transplanted to evaluate fertilization and pregnancy rates (NOA, 15 fresh and 123 thawed; OA, 59 fresh and 393 thawed). Finally, of the 101 ICSI cycles performed for NOA patients, 56 fresh-sperm cycles and 45 thawed-sperm cycles were performed to evaluate ICSI outcomes. Fertilization rates and two-pronuclear (2PN) fertilization rates were significantly lower in the NOA group than in the OA group. However, the 2PN cleavage rate, the high-quality embryo rate, the blastocyst formation rate, and the available blastocyst rate showed no significant intergroup differences. In addition, the pregnancy outcomes and neonatal health conditions were statistically similar. Finally, compared with thawed sperm, the fresh-sperm group had a higher 2PN fertilization rate and a higher high-quality embryo rate. However, blastocyst formation and available blastocyst rates were similar between the two groups. Patients with NOA achieved the same favorable results in embryo development, clinical pregnancy, and live birth capability as did OA patients. Neonatal conditions were not affected by type of azoospermia (NOA versus OA). For patients with NOA, fresh testicular sperm is superior to frozen-thawed testicular sperm in embryo development as evaluated at the cleavage stage, but we find no superiority evaluating at the stage of blastocyst formation.
Reprogramming Cancer into Antigen-Presenting Cells as a Novel Immunotherapy
Authors: Linde Miles H. , Fan Amy C. , Köhnke Thomas , Trotman-Grant Aaron C. , Gurev Sarah F. , Phan Paul , Zhao Feifei , Haddock Naomi L. , Nuno Kevin A. , Gars Eric J. , Stafford Melissa , Marshall Payton L. , Dove Christopher G. , Linde Ian L. , Landberg Niklas , Miller Lindsay P. , Majzner Robbie G. , Zhang Tian Yi , Majeti Ravindra
Published: March 2023

Therapeutic cancer vaccination seeks to elicit activation of tumor-reactive T cells capable of recognizing tumor-associated antigens (TAA) and eradicating malignant cells. Here, we present a cancer vaccination approach utilizing myeloid-lineage reprogramming to directly convert cancer cells into tumor-reprogrammed antigen-presenting cells (TR-APC). Using syngeneic murine leukemia models, we demonstrate that TR-APCs acquire both myeloid phenotype and function, process and present endogenous TAAs, and potently stimulate TAA-specific CD4+ and CD8+ T cells. In vivo TR-APC induction elicits clonal expansion of cancer-specific T cells, establishes cancer-specific immune memory, and ultimately promotes leukemia eradication. We further show that both hematologic cancers and solid tumors, including sarcomas and carcinomas, are amenable to myeloid-lineage reprogramming into TR-APCs. Finally, we demonstrate the clinical applicability of this approach by generating TR-APCs from primary clinical specimens and stimulating autologous patient-derived T cells. Thus, TR-APCs represent a cancer vaccination therapeutic strategy with broad implications for clinical immuno-oncology.

Significance:

Despite recent advances, the clinical benefit provided by cancer vaccination remains limited. We present a cancer vaccination approach leveraging myeloid-lineage reprogramming of cancer cells into APCs, which subsequently activate anticancer immunity through presentation of self-derived cancer antigens. Both hematologic and solid malignancies derive significant therapeutic benefit from reprogramming-based immunotherapy.

This article is highlighted in the In This Issue feature, p. 1027
A Novel Ubiquitin Complex Regulates Aneuploid Epithelial Tumors by Moderating an Integrated Stress Response
Authors: Leli Nektaria Maria , Koumenis Constantinos
Published: March 2023

Tumor fitness coessentiality gene analysis that aims to expand the repertoire of druggable targets reveals a novel ubiquitin ligase complex, the BICR6 module. Along with the other complex members (UBA6, KCMF1, and UBR4), BIRC6 selectively contributes to the survival of a subset of epithelial tumors with a high degree of aneuploidy by ubiquitinating and suppressing HRI, a component of the integrated stress response adaptive pathway.

See related article by Cervia et al., p. 766 (2).
Cushing’s Syndrome in Adenocarcinoma of Lung Responding to Osilodrostat
Authors: Heleno Caio Teixeira , Hong Seung Pyo D. , Cho Hyung-Gyo , Kim Min Jeong , Park Yeonggyeong , Chae Young Kwang
Published: March 2023

Cushing’s syndrome (CS), secondary to paraneoplastic syndrome, is more commonly seen in small cell lung cancer but never before reported in epidermal growth factor receptor-mutated adenocarcinoma of the lung. Here, we present a case of a patient whose symptoms of hypokalemia, hypertension, and progressive abnormal glucose levels led to further workup that revealed adrenocorticotropic hormone-dependent hypercortisolism. Her cortisol levels dropped after 1 month of osilodrostat treatment, while lung cancer was treated with osimertinib. The use of osilodrostat in paraneoplastic CS has been previously reported in only 3 patients.
Incidence and Types of Fetal Chromosomal Abnormalities in First Trimester of Thai Pregnant Women between Miscarriages and Intrauterine Survivals
Authors: Parinayok Rachanee , Areesirisuk Prapatsorn , Chareonsirisuthigul Takol , Buchachat Warakorn , Rerkamnuaychoke Budsaba
Published: March 2023

Abortion is a common pregnancy complication. Fetuses with several types of chromosomal abnormalities are aborted during the first trimester, while others have a better chance of surviving. This research aims to study and compare the incidence and types of fetal chromosomal abnormalities during the first trimester of Thai pregnant women between miscarriages and intrauterine survivals. Cytogenetic and BACs-on-Beads™ assays were assessed from 2010 to 2020 in Ramathibodi Hospital using first trimester samples of 265 chorionic villi as a retrospective study. Chromosomal abnormalities were observed in 135 cases (50.94%) including 38.11% miscarriages and 12.83% intrauterine survivals. In total, 75.56% single autosomal trisomies, 18.52% sex chromosome aneuploidies, 5.19% double aneuploidies, and 0.74% structural abnormalities were detected. In miscarriages, all chromosomes were involved in abnormalities except chromosomes 1, 5, 8, 9, 11, and 17, while survivals had only trisomy 13, 18, 21, and sex chromosome aneuploidy. Trisomy 16 and 18 were the most common abnormalities in miscarriages and intrauterine survivals, respectively. The highest rate of chromosomal aberrations was demonstrated in 8–9+6 and 12–13+6 weeks of gestation in miscarriages and intrauterine survivals, respectively. Correlation between chromosomal abnormalities and maternal age <35 years and ≥35 years was significant (p < 0.05) in intrauterine survival and first trimester groups.
Multimorbidity and Functional Disability among Older Adults: The Role of Inflammation and Glycemic Status – An Observational Longitudinal Study
Authors: Botoseneanu Anda , Markwardt Sheila , Quiñones Ana R.
Published: March 2023

Introduction: Specific multimorbidity combinations, in particular those including arthritis, stroke, and cognitive impairment, have been associated with high burden of activities of daily living (ADL)-instrumental activities of daily living (IADL) disability in older adults. The biologic underpinnings of these associations are still unclear. Methods: Observational longitudinal study using data from the Health and Retirement Study (N = 8,618, mean age = 74 years, 58% female, 25% non-white) and negative binomial regression models stratified by sex to evaluate the role of inflammatory and glycemic biomarkers (high-sensitivity C-reactive protein (hs-CRP) and HbA1c) in the association between specific multimorbidity combinations (grouped around one of eight index diseases: arthritis, cancer, cognitive impairment, diabetes, heart disease, hypertension, lung disease, and stroke; assessed between 2006 and 2014) and prospective ADL-IADL disability (2 years later, 2008–2016). Results were adjusted for sociodemographic characteristics, body mass index, number of coexisting diseases, and baseline ADL-IADL score. Results: Multimorbidity combinations indexed by arthritis (IRR = 1.1, 95% CI = 1.01–1.20), diabetes (IRR = 1.19, 95% CI = 1.09–1.30), and cognitive impairment (IRR = 1.11, 95% CI = 1.01–1.23) among men and diabetes-indexed multimorbidity combinations (IRR = 1.07, 95% CI = 1.01–1.14) among women were associated with higher ADL-IADL scores at increasing levels of HbA1c. Across higher levels of hs-CRP, multimorbidity combinations indexed by arthritis (IRR = 1.06, 95% CI = 1.02–1.11), hypertension (IRR = 1.06, 95% CI = 1.02–1.11), heart disease (IRR = 1.06, 95% CI = 1.01–1.12), and lung disease (IRR = 1.14, 95% CI = 1.07–1.23) were associated with higher ADL-IADL scores among women, while there were no significant associations among men. Conclusion: The findings suggest potential for anti-inflammatory management among older women and optimal glycemic control among older men with these particular multimorbidity combinations as focus for therapeutic/preventive options for maintaining functional health.
Leukocytoclastic Vasculitis Associated with Adalimumab Therapy for Crohn’s Disease
Authors: Xu Lankai Cathy , Grandhe Sirisha , Marsano Joseph G
Published: March 2023

Leukocytoclastic vasculitis (LCV) is rarely associated with anti-tumor necrosis factor [TNF] α therapy. We report a 22-year-old man with new onset of a pustular rash on his bilateral upper and lower extremities while on adalimumab therapy for Crohn’s disease. Skin biopsy of the affected area showed perivascular extravasation of erythrocytes, neutrophils, eosinophils and vascular damage surrounding blood vessels associated with fibrin, consistent with LCV. Patient was treated with topical steroids and subsequently transitioned to ustekinumab therapy with follow-up colonoscopy showing minimal active disease. Our report highlights the association of a unique dermatologic autoimmune manifestation with TNF-targeted therapy in a patient with Crohn’s disease.
Development of Acute Transverse Myelitis following COVID-19 Infection: A Review on the Potential Pathways
Authors: Farrokhi Majid Reza , Iravanpour Farideh , Nejabat Negar
Published: March 2023

Background: Acute transverse myelitis (ATM) is a rare neurological disorder in adults characterized by localized inflammation of gray and white matter in one or more contiguous spinal cord segments in the absence of a compressive injury. Several reports have connected the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to the pathophysiology of ATM. Summary: Direct invasion of the spinal cord, cytokine storm, or an autoimmune response are the possible pathways by which the SARS-CoV-2 virus can affect the spinal cord and lead to ATM. Direct invasion is facilitated by the presence of angiotensin-converting enzyme 2 (ACE2) receptors on the membranes of the spinal cord neurons. Cytokine storm syndrome could be derived from elevated levels of several immunological factors following severe involvement with coronavirus disease 2019 (COVID-19). Finally, autoimmune responses can cause post-infectious ATM through several hypothesized processes, including molecular mimicry, epitope spreading, bystander activation, and polyclonal B-cell activation. Key Messages: COVID-19-induced ATM is mostly a longitudinally-extensive ATM (LEATM), in which more spinal cord segments are damaged, which results in a worse sequel compared to short-segment ATM. Therefore, it is suggested that COVID-19 patients, particularly severe cases, be followed up for a probable incidence of ATM, even long after recovery from the disease and elimination of the virus from the host, because an early diagnosis and effective therapy may stop the spread of inflammation to adjacent segments.
A new species of Eugenia sect. Eugenia (Myrtaceae) from Atlantic Forest in São Paulo, Brazil
Authors: MAZINE FIORELLA F. , VALDEMARIN KARINNE SAMPAIO , GIARETTA AUGUSTO , SOBRAL MARCOS
Published: March 2023

Eugenia aeterna, a new species of Eugenia sect. Eugenia is described and illustrated. It has been recorded in the Ombrophilous Forest of the Atlantic Forest Domain in the municipalities of Caraguatatuba and Ubatuba, São Paulo state, Brazil. The new species is apparently related to Eugenia ligustrina and E. longipedunculata being distinct from both by: cataphylls persistent until mature twigs, even in the vegetative phase of the plant (versus cataphylls deciduous); puberulent or pilose hypanthium (vs. glabrous hypanthium) and calyx lobes reflexed and appressed to the fruit (vs. calyx lobes erect in the fruit). Following IUCN red list criteria, Eugenia aeterna is classified Least Concern. Additionally, a distribution map is provided.
Characterization of the Morning Transition over the Gentle Slope of a Semi-Isolated Massif
Authors: Farina Sofia , Marchio Mattia , Barbano Francesco , di Sabatino Silvana , Zardi Dino
Published: March 2023

This paper investigates the surface-layer processes associated with the morning transition from nighttime downslope winds to daytime upslope winds over a semi-isolated massif. It provides an insight into the characteristics of the transition and its connection with the processes controlling the erosion of the temperature inversion at the foot of the slope. First, a criterion for the identification of days prone to the development of purely thermally driven slope winds is proposed and adopted to select five representative case studies. Then, the mechanisms leading to different patterns of erosion of the nocturnal temperature inversion at the foot of the slope are analyzed. Three main patterns of erosion are identified: the first is connected to the growth of the convective boundary layer at the surface, the second is connected to the descent of the inversion top, and the third is a combination of the previous two. The first pattern is linked to the initiation of the morning transition through surface heating, and the second pattern is connected to the top-down dilution mechanism and so to mixing with the above air. The discriminating factor in the determination of the erosion pattern is identified in the partitioning of turbulent sensible heat flux at the surface.

Significance Statement

The purpose of this study is to improve our understanding of the thermally driven slope circulations with a focus on the unsteady processes associated with the morning transition and the erosion patterns of the nocturnal temperature inversion, so far in the literature less investigated and understood than the evening transition. Understanding this diurnal process will advance our abilities to model it and to improve the accuracy of weather forecasting in complex terrain.
Perioperative renal function change after transcatheter aortic valve replacement: A single-center retrospective study in China
Authors: Dong Zhe , Yao Jing , Li Rui , Liu Xinmin , Yuan Fei , Luo Taiyang , Zhang Qian , Song Guangyuan
Published: March 2023

Background

In recent years, the kidney function after Transcatheter Aortic Valve Replacement (TAVR) has gradually become a hot spot that arouse extensive attention.Our study is aimed to evaluate the incidence and predictors of acute kidney recovery (AKR) after TAVR.

Methods

A total of 102 patients undergoing TAVR in Beijing Anzhen Hospital from June 2021 to March 2022 were enrolled in our study. Patients were divided into AKR group ( n = 54), unchanged group ( n = 40) and acute kidney injury (AKI) group ( n = 8) based on the percent change of estimated glomerular filtration rate (eGFR). Univariate analysis was used to compare the differences in general clinical characteristics and other related indicators between the three groups to analyze the risk factors of AKR.

Results

The incidence of AKR was 53% (54/102) after TAVR. Multivariate analysis showed that the incidence of age and proportion of severe NYHA class (III or IV) was significantly higher in the AKR group while renal dysfunction (eGFR <60 mL/min/1.73 m2) was lower. Besides, fluid management/volume therapy was significantly different among the three groups.

Conclusions

AKR is a generalizable phenomenon occurring frequently after TAVR. The age, proportion of severe NYHA class and the baseline renal function are independent predictors of AKR events in patients with severe aortic stenosis undergoing TAVR.
Evaluating the efficacy of CGRP mAbs and gepants for the preventive treatment of migraine: A systematic review and network meta-analysis of phase 3 randomised controlled trials
Authors: Haghdoost Faraidoon , Puledda Francesca , Garcia-Azorin David , Huessler Eva-Maria , Messina Roberta , Pozo-Rosich Patricia
Published: March 2023

Background

Several novel treatments targeting the calcitonin gene-related peptide pathway have been developed for migraine. We evaluated the efficacy of these medications, including atogepant, rimegepant, erenumab, eptinezumab, fremanezumab, and galcanezumab, for the prevention of migraine via network meta-analysis.

Methods

Databases, including MEDLINE via PubMed, EMBASE, and Cochrane central, were systematically reviewed, and all eligible phase 3 randomised controlled trials were included.

Results

Nineteen studies (n = 14,584 participants) were included. Studies included episodic (n = 11) and chronic (n = 4) migraine or both (n = 4). All interventions, except for eptinzumab 30 mg, significantly reduced mean monthly migraine days compared to placebo. All medications had a higher ≥50% responder rate than placebo and results were statistically significant in those with the subcutaneous or intravenous route of administrations, but not with the oral one. All medications significantly reduced mean monthly headache days, although no data for this outcome was available for rimegepant, and mean monthly acute medication days, with no data for eptinezumab.

Conclusion

The results show that medications targeting calcitonin gene-related peptide were effective in preventing migraine compared to placebo. Considering limitations of single studies, different populations such as episodic and chronic migraine, and the absence of head-to-head trials, all novel treatments decreased mean monthly migraine and headache days, and showed higher 50%, 75% and 100% responder rates than placebo. Trial registration: PROSPERO registration: CRD42022310579
Patient perspectives on peritoneal dialysis (PD) and the PD catheter: Strategies and Solutions
Authors: Fissell Rachel B , Wysocki Matthew , Bonnet Kemberlee , Abifaraj Farah , Cavanaugh Kerri L , Nair Devika , Umeukeje Ebele M , Wild Marcus G , Liddell Peter , Spangler Matthew , Schlundt David
Published: March 2023

Introduction:

Peritoneal dialysis (PD) catheter complications reduce quality of life and increase risks for hospitalizations, for unplanned transitions to haemodialysis and for death. Patient PD catheter management is crucial for safe, sustained PD. Patient perspectives on strategies for living with PD and using a PD catheter may inform efforts to reduce PD catheter complications, increase individual patient PD modality persistence, and thus increase overall home dialysis prevalence.

Methods:

We interviewed 32 adult PD patients in Nashville, Tennessee. Qualitative analyses included (1) isolation of themes, (2) development of a coding system and (3) creation of a conceptual framework using an inductive–deductive approach.

Results:

Challenges identified by patients as important included drain pain, difficulty eating and sleeping, and fear of peritonitis. Coping strategies included repositioning while draining, adjusting eating patterns, and development of PD patient and helper knowledge and confidence, especially at home after initial training. Patients described a trial-and-error iterative process of trying multiple strategies with input from multiple sources, which led to individualised solutions.

Conclusions:

The trial-and-error process may be crucial for maintaining PD. Individual patient success with PD may be promoted by creating expectations during training that a solution may require multiple attempts, and by a reimbursement policy that supports robust nursing support for safe progression through the trial-and-error process, particularly in the first few months for incident patients. Interventions to support patient motivation and optimal coping behaviour may also support an increase in PD modality duration for individual patients, and thus increase overall PD prevalence.
Upper Tract Urinary Carcinoma: A Unique Immuno-Molecular Entity and a Clinical Challenge in the Current Therapeutic Scenario
Authors: Mazzaschi Giulia , Giudice Giulia Claire , Corianò Matilde , Campobasso Davide , Perrone Fabiana , Maffezzoli Michele , Testi Irene , Isella Luca , Maestroni Umberto , Buti Sebastiano
Published: March 2023

Urothelial carcinoma (UC) is the most frequent malignancy of the urinary tract, which consists of bladder cancer (BC) for 90%, while 5% to 10%, of urinary tract UC (UTUC). BC and UTUC are characterized by distinct phenotypical and genotypical features as well as specific gene- and protein- expression profiles, which result in a diverse natural history of the tumor. With respect to BC, UTUC tends to be diagnosed in a later stage and displays poorer clinical outcome. In the present review, we seek to highlight the individuality of UTUC from a biological, immunological, genetic-molecular, and clinical standpoint, also reporting the most recent evidence on UTUC treatment. In this regard, while the role of surgery in nonmetastatic UTUC is undebated, solid data on adjuvant or neoadjuvant chemotherapy are still an unmet need, not permitting a definite paradigm shift in the standard treatment. In advanced setting, evidence is mainly based on BC literature and retrospective studies and confirms platinum-based combination regimens as bedrock of first-line treatment. Recently, immunotherapy and target therapy are gaining a foothold in the treatment of metastatic disease, with pembrolizumab and atezolizumab showing encouraging results in combination with chemotherapy as a first-line strategy. Moreover, atezolizumab performed well as a maintenance treatment, while pembrolizumab as a single agent achieved promising outcomes in second-line setting. Regarding the target therapy, erdafitinib, a fibroblast growth factor receptor inhibitor, and enfortumab vedotin, an antibody-drug conjugate, proved to have a strong antitumor property, likely due to the distinctive immune-genetic background of UTUC. In this context, great efforts have been addressed to uncover the biological, immunological, and clinical grounds in UTUC patients in order to achieve a personalized treatment.
Successful treatment of non-Langerhans cell histiocytosis with the MEK inhibitor trametinib: a multicenter analysis
Authors: Aaroe Ashley , Kurzrock Razelle , Goyal Gaurav , Goodman Aaron M. , Patel Harsh , Ruan Gordon , Ulaner Gary , Young Jason , Li Ziyi , Dustin Derek , Go Ronald S. , Diamond Eli L. , Janku Filip
Published: March 2023

Erdheim-Chester disease (ECD) and Rosai-Dorfman disease (RDD) are rare non-Langerhans cell histiocytoses (non-LCHs), for which therapeutic options are limited. MAPK pathway activation through BRAFV600E mutation or other genomic alterations is a histiocytosis hallmark and correlates with a favorable response to BRAF inhibitors and the MEK inhibitor cobimetinib. However, there has been no systematic evaluation of alternative MEK inhibitors. To assess the efficacy and safety of the MEK inhibitor trametinib, we retrospectively analyzed the outcomes of 26 adult patients (17 with ECD, 5 with ECD/RDD, 3 with RDD, and 1 with ECD/LCH) treated with orally administered trametinib at 4 major US care centers. The most common treatment-related toxicity was rash (27% of patients). In most patients, the disease was effectively managed at low doses (0.5-1.0 mg trametinib daily). The response rate of the 17 evaluable patients was 71% (73% [8/11] without a detectable BRAFV600E achieving response). At a median follow-up of 23 months, treatment effects were durable, with a median time-to-treatment failure of 37 months, whereas the median progression-free and overall survival were not reached (at 3 years, 90.1% of patients were alive). Most patients harbored mutations in BRAF (either classic BRAFV600E or other BRAF alterations) or alterations in other genes involved in the MAPK pathway, eg, MAP2K, NF1, GNAS, or RAS. Most patients required lower than standard doses of trametinib but were responsive to lower doses. Our data suggest that the MEK inhibitor trametinib is an effective treatment for ECD and RDD, including those without the BRAFV600E mutation.
Coupling magnetic levitation of graphene oxide–protein complexes with blood levels of glucose for early detection of pancreatic adenocarcinoma
Authors: Quagliarini Erica , Caputo Damiano , Cammarata Roberto , Caracciolo Giulio , Pozzi Daniela
Published: March 2023

Introduction

Pancreatic adenocarcinoma (PDAC) has a poor prognosis since often diagnosed too late. Dyslipidemia and hyperglycemia are considered risk factors, but the presence of the tumor itself can determine the onset of these disorders. Therefore, it is not easy to predict which subjects with diabetes or dyslipidemia will develop or have already developed PDAC. Over the past decade, tests based on the use of nanotechnology, alone or coupled with common laboratory tests (e.g., hemoglobin levels), have proven useful in aiding the diagnosis of PDAC. Tests based on magnetic levitation (MagLev) have demonstrated high diagnostic accuracy in compliance with the REASSURED criteria. Here, we aimed to assess the ability of the MagLev test in detecting PDAC when coupled with the blood levels of glycemia, cholesterol, and triglycerides.

Methods

Blood samples from 24 PDAC patients and 22 healthy controls were collected. Human plasma was let to interact with graphene oxide (GO) nanosheets and the emerging coronated systems were put in the MagLev device. Outcomes from Maglev experiments were coupled to glycemia, cholesterol, and triglycerides levels. Linear discriminant analysis (LDA) was carried out to evaluate the classification ability of the test in terms of specificity, sensitivity, and global accuracy. Statistical analysis was performed with Matlab (MathWorks, Natick, MA, USA, Version R2022a) software.

Results

The positions of the levitating bands were measured at the starting point (i.e., as soon as the cuvette containing the sample was subjected to the magnetic field). Significant variations in the starting position of levitating nanosystems in controls and PDACs were detected. The combination of the MagLev outcomes with the blood glycemic levels returned the best value of global accuracy (91%) if compared to the coupling with those of cholesterol and triglycerides (global accuracy of ~ 77% and 84%, respectively).

Conclusion

If confirmed by further studies on larger cohorts, a multiplexed Maglev-based nanotechnology-enabled blood test could be employed as a screening tool for PDAC in populations with hyperglycemia.
Screening of prognosis-related Immune cells and prognostic predictors in Colorectal Cancer Patients
Authors: Deng Shuangshuang , Zhu Qiping , Chen Hongyan , Xiao Tianyu , Zhu Yinshen , Gao Jinli , Li Qing , Gao Yong
Published: March 2023

Objective

To accurately screen potential immune cells that can predict the survival of colorectal cancer (CRC) patients and identify related prognostic predictors.

Methods

The sample data of CRC patients were downloaded from the GEO database as a training set to establish a prognosis-scoring model and screen prognosis-related immune cells. The sample data of CRC patients from the TCGA database were used as the validation set. Simultaneously, cancer tissue samples from 116 patients with CRC diagnosed pathologically in Shanghai Dongfang Hospital were collected to analyze the relationship of prognosis-related immune cells with patients’ survival, and clinical and pathological parameters, and to screen prognostic predictors.

Results

Prognosis-related immune cells screened from GEO and TCGA databases mainly included Follicular Helper T cells (Tfh), Monocytes and M2 Macrophages. In the training set, the 2,000- and 4,000-day survival rates were 48.3% and 10.7% in the low-risk group (N = 234), and 42.1% and 7.5% in the high-risk group (N = 214), respectively. In the validation set, the 2,000- and 4,000-day survival rates were 34.8% and 8.6% in the low-risk group (N = 187), and 28.9% and 6.1% in the high-risk group (N = 246), respectively. The prognosis of patients in the high-risk group was worse than that in the low-risk group (P < 0.05). Furthermore, the screened primary prognostic predictors were CD163 and CD4 + CXCR5. CD163 protein expression was distributed in Monocytes and M2 Macrophages. The 1,000- and 2,000-day survival rates were 56.1% and 7.0% in the CD163 low-expression group, and 40.7% and 1.7% in the high-expression group (N = 214), respectively, showing a worse prognosis in the high-expression group than that in the low-expression group. Meanwhile, the immune marker CD4 + CXCR5 could identify Tfh. The 1,000- and 2,000-day survival rates were 63.9% and 5.6% in the CD4 + CXCR5 high-expression group, and 33.3% and 2.8% in the low-expression group (N = 214), respectively, with a better prognosis in the high-expression group than that in the low-expression group.

Conclusion

Prognostic-related immune cells of CRC mainly include Tfh cells, Monocytes and M2 Macrophages. Monocytes and M2 Macrophages correlate negatively, while Tfh cells correlate positively with the prognosis of CRC patients. Immune markers CD163 and CD4 + CXCR5 can be considered as the prognostic predictors of CRC with clinical value of the application.