bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimized Design of a Backbone Network for Public Transportation in Montevideo, Uruguay
Authors: Risso Claudio , Nesmachnow Sergio , Faller Germán
Published: November 2023

This article presents the application of exact and metaheuristic approaches to the problem of designing the backbone network of a hierarchically public transportation system for Montevideo, Uruguay. This is a very relevant problem in nowadays smart cities, as it accounts for many social and environmental impacts and also affects the dynamics of the cities. The design of the proposed backbone network is conceived in combination with the bus network, with the main objective of improving the overall quality of service and reducing travel times. Three different variants of the problem are solved, considering different design premises. Exact solvers are proposed for simpler variants of the problem, which account for maximum resilience and bounded travel times. An evolutionary algorithm is proposed for a multiobjectie version of the problem that optimizes cost and quality of service. The main results indicate that the computed optimized designs provide reduced end-to-end travel times, which improve up to five times over the current system, and are economically viable to be implemented.
Endophytic Development of the Entomopathogenic Fungus Beauveria bassiana Reduced the Development of Galls and Adult Emergence of Leptocybe invasa in Susceptible Eucalyptus
Authors: Nunes Thomas Vieira , Rodrigues Jovielly Neves , Pinto Ismael O. , Pimenta Raphael S. , Sarmento Maíra Ignacio , Silva Ricardo S. , Souza Philipe G. C. , de Souza Danival J. , Joseph Louis A. , de O. Souza Maria L. , Sarmento Renato A.
Published: November 2023

Eucalyptus cultivation in arid regions is limited by the losses caused by the galling wasp, Leptocybe invasa Fisher and La Salle, 2004 (Hymenoptera: Eulophidae); it oviposits below the epidermis of the central veins, mainly on seedlings in nurseries and/or young plants in the field, causing galls where the insects develop. Galls modify the normal circulation of sap in vascular tissues, ultimately affecting the development of infested plants and resulting in slower growth. Eucalyptus genotypes tolerant to water stress are susceptible to L. invasa, and this pest has no effective control methods. Here, we aimed to (i) determine the initial infestation dynamics of L. invasa in Eucalyptus plants inoculated with Beauveria bassiana and (ii) identify any change in the development of galls. B. bassiana-inoculated seedlings were infested with L. invasa. Susceptible plants were unaffected, and gall development was negatively affected at multiple stages. A fungal solution containing B. bassiana reduced gall development and the emergence of L. invasa adults in eucalyptus plants at considerable rates. The total number of adult emergence holes was 89.74% fewer for inoculated plants when compared to uninoculated plants. These results can help develop B. bassiana products to control L. invasa in susceptible eucalyptus plants for improved, sustainable forestry.
Discrepancies in Mapping Sustainable Development Goal 3 (Good Health and Well-Being) Research: A Comparative Analysis of Scopus and Dimensions Databases
Authors: Raman Raghu , Nair Vinith Kumar , Nedungadi Prema
Published: November 2023

Understanding the alignment and contributions of scientific research to the Sustainable Development Goals (SDGs) is essential for guiding global progress toward these critical targets. In this context, the study focuses on SDG 3 (Good Health and Well-Being), as it dominates the most researched SDG literature, thus providing a meaningful representation of the broader SDG research landscape. The comprehensive examination of the SDG 3-related research from 2018 to 2022 highlights significant discrepancies in publications mapped to SDG 3 between the two largest databases, Scopus and Dimensions. Despite previous studies showing Dimensions to have more coverage, the present analysis finds Scopus to report 124% more publications in this area. These discrepancies extend across various levels, including country, funder, institution, journal, and author, and have substantial implications for stakeholders relying on these data. Notably, contrasting cluster themes are discovered, with Dimensions revealing five integrative clusters and Scopus focusing on six specialized ones. This discrepancy can affect global research attention, funding allocation, institutional recognition, and SDG journal alignment. The findings emphasize the need for standardization and transparency in SDG mapping methodologies, especially as the 2030 target year approaches and progress on many 2030 SDG targets is lagging. It also highlights the importance of acknowledging and understanding these disparities at various levels of the research ecosystem. The study raises questions about similar discrepancies in other SDGs and necessitates a broader analysis that might include more databases and refine publication types. It serves as a cautionary reminder to the scientific community, policymakers, and other stakeholders about the importance of careful and comprehensive evaluation when mapping publications to SDGs.
Engagement and Burnout in Times of COVID-19: A Comparative Analysis Based on Healthy Organizational Practices
Authors: Acuña-Hormazábal Álvaro , Dávila-Vera Macarena , Mendoza-Llanos Rodolfo , Maureira-Meneses Sebastian , Pons-Peregort Olga
Published: November 2023

The COVID-19 pandemic has prompted a closer examination of organizational management’s role in facilitating positive outcomes in the workplace. This study explores the relationship between perceptions of healthy organizational practices (HOP) and levels of engagement and burnout among workers. We applied surveys to 213 workers (66% women) from different sectors (health, education, and municipal) in a south-center region of Chile. The results align with the existing literature, showing a positive correlation between HOP and engagement and a negative correlation between HOP and burnout in the total sample. ANOVA analysis revealed significant differences among organizations. Workers in the education organization reported higher perceptions of healthy practices, as well as higher engagement and lower burnout levels. These findings highlight the education organization as a potential exemplar of a healthy workplace. The study underscores the importance of healthy practices in promoting employee well-being and offers insights into sector-specific dynamics. Organizations, particularly in health and municipal sectors, should prioritize strategies that foster healthy work environments and mitigate the risk of burnout among their workforce. The findings of this research corroborate that HOP contributes to the sustainability of a healthy workplace. It discusses the need to investigate complementary variables that will allow the development of a model for healthy and sustainable organizations tailored to the specific organizations and the culture of its people.
A Novel Process for the On-Site Preparation and Application of Polyferric Chloride (PFC) for Surface Water Treatment
Authors: Gkotsis Petros , Mitrakas Manassis , Zouboulis Anastasios
Published: November 2023

This is the first study to describe a novel, patented process for the on-site synthesis and subsequent direct utilisation of Polyferric Chloride (PFC) at low Fe concentration dosing, which aims to facilitate the potential replacement of Polyaluminium Chloride (PAC) during surface water treatment (e.g., from reservoirs) for drinking water production. For this purpose, the PFC was synthesised and subsequently used as a coagulant in simulated surface water samples under different synthesis and coagulation/flocculation conditions, namely for different pre-hydrolysed Fe concentrations, pre-hydrolysis pH, coagulation pH, and flocculation times. The effectiveness of PFC was examined mainly in terms of total organic carbon (TOC) removal and the residual Fe concentration. The obtained results showed that the pre-hydrolysed Fe concentration at 0.5 ± 0.25%, pre-hydrolysis at pH 2.5 ± 0.25, coagulation at pH 5.5–7.0 and a flocculation time of 5 min could result in the highest TOC removal (i.e., residual values < 0.60 mg/L) and the lowest residual Fe concentration (<5 μg Fe/L), which is acceptable for a water quality assessment. These values are also substantially lower when compared to the respective TOC and residual metal concentrations using PAC (usually, the relevant obtained values are around TOC > 1 mg/L and Al > 50 μg/L).
Application of the Decision-Making Trial and Evaluation Laboratory Method to Assess Factors Influencing the Development of Cycling Infrastructure in Cities
Authors: Trembecka Anna , Ginda Grzegorz , Kwartnik-Pruc Anita
Published: November 2023

Sustainable development and environmental considerations have resulted in many cities around the world recognising the importance of non-motorised modes of transport. Problems related to the proper development and maintenance of cycling infrastructure have already been the subject of various studies. However, they have mainly dealt with the identification of factors influencing the development of cycle paths and the optimisation of the design of safe and comfortable cycle routes. The influence of individual factors on each other and on the development of cycling infrastructure has not been studied. The research aims of this article are to identify which factors influence the development of bicycle infrastructure, their role and interdependence, and their prioritisation. It also looks at whether there are differences between the opinions of bicycle users and experts professionally involved in the development of bicycle paths in assessing the importance of the factors indicated. As a result of the study, eight factors influencing the development of bicycle infrastructure were identified. Based on the opinions of cyclists and experts, the nature of each factor was analysed. Taking into account the complex relationships between the factors, the key factors contributing to the development of bicycle infrastructure were shown: (1) the planning of bicycle paths, taking into account the separation of individual paths and their continuity, consistency, and length; (2) legal regulations promoting cycling in terms of transportation policy; (3) the elimination of obstacles; and (4) the design of bicycle paths, taking into account the safety, space management, terrain, and attractiveness of the surroundings. The results for both groups of respondents were compared. They indicate that both groups of respondents reported the same factors as the most important, with the only differences being in the order of the importance of the factors. The academic value of this work lies in showing the usability of the underrated original version of DEMATEL methodology in the considered area for key factors. The practical significance of this paper is the provision of a rather simple, yet reliable, tool for addressing the complexity of interrelated issues that make the development of urban infrastructure a cumbersome task.
The Impact Mechanism of Work Experience on the Income of Flexible Workers: Evidence from China
Authors: Yang He , Li Changan , Sun Zhaoxing
Published: November 2023

Numerous workers have adopted the flexible working approach due to its accommodating and job-sharing features, which play a key role in easing the employment pressure and maintaining socio-economic sustainability in China. This paper will study the impact of work experience on the income of flexible workers and the characteristics of impact mechanism. We use micro survey data from CFPS2020 to analyze flexible workers in China. This study found that, firstly, while the feature of flexible employment may be very different from formal employment, its relationship with work experience and income is as significantly correlated as it is for formal employment with a rise–fall, inverted U-shaped trend, but the return on work experience is lower than the employment level in the labor market. Secondly, there is an income gap between genders, especially in physical labor-intensive forms of flexible work; women can gradually narrow the gender income gap through continuous learning. Thirdly, the return on work experience in the older age group is lower than that in the youth group of flexible workers, and this may be due to the dynamic evolution of the elimination of existing work experience and the accumulation of new work experience. Fourthly, in contrast with the findings of the labor market as a whole, the trend of work experience return in flexible employment is reversed; the low-income group’s return rate of work experience is higher than that of the high-income group, and it can be seen that flexible employment is conducive to the development of new and young workers.
Enhancement of Liquid Hot Water Pretreatment on Corn Stover with Ball Milling to Improve Total Sugar Yields
Authors: Ji Guanya , Zhang Bo , Niu Qijian , Liu Yuxin , Yang Qizhi
Published: November 2023

Conversion of the lignocellulosic biomass to bioethanol contributes to the reduction of greenhouse gas emissions, enhancement of energy security, utilization of waste materials, and the promotion of sustainable agricultural practices. In this study, we report the effect of combining ball milling followed by liquid hot water (LHW) pretreatment of corn stover to lower the amount of enzyme required while also greatly increasing the recovery of xylose in fermentable form compared to either pretreatment alone. Short-duration ball milling for 60 min reduces the particle size of corn stover to 37.3 μm; however, the glucose only increased to 47% compared to 32% for unpretreated corn stover. In contrast, liquid hot water pretreatment alone can achieve increasing enzyme hydrolysis yields of cellulose from 49% to 93% as the pretreatment severity factor is increased from 3.24 to 4.41. However, the xylose yield decreased to 36% due to the fact that a considerable part of the xylose was degraded into furfural and humins. Surprisingly, the combination of mild ball milling (30 min) followed by mild liquid hot water pretreatment (190 °C, 15 min) could achieve both high glucose (83%) and xylose (72%) yields for a total sugar yield of 79%, theoretically. Thus, combining ball milling with liquid hot water pretreatment allows for milder conditions for both processes that lead to enhanced cellulose conversion without sacrificing xylose to degradation, which hinders enzymatic hydrolysis.
Electrification of Motorway Network: A Methodological Approach to Define Location of Charging Infrastructure for EV
Authors: Colombo Cristian Giovanni , Borghetti Fabio , Longo Michela , Foiadelli Federica
Published: November 2023

Environmental issues have reached global attention from both political and social perspectives. Many countries and companies around the world are adopting measures to help change current trends. Awareness of decarbonization in the transportation sector has led to an increasing development of energy storage systems in recent years, especially for ground vehicles. Batteries, due to their high efficiency, are one of the most attractive energy storage systems for vehicle propulsion. As for road vehicles, the growing interest in Electric Vehicles (EVs) is motivated by the fact that they reduce local emissions compared to traditional Internal Combustion Engine (ICE) vehicles. The purpose of the paper is to present a study on how to plan and implement vehicle charging infrastructure on motorways. In particular, a specific road in Italy is analyzed: the motorway A1 from Milan to Naples with a length of about 800 km. This motorway can be considered representative because it passes through some of Italy’s most important cities and regions and may represent the backbone of Italy. A useful model for defining the optimal location of electric vehicle charging stations is presented within the paper. Starting with the data on the average daily traffic flows passing through the main nodes of the motorways section, the demand for the potential vehicles needed to define the number and dimension of charging stations and provide an adequate supply is estimated. The analysis was performed considering five-time horizons (year 2022 to year 2025) and four Scenarios involving the installation of 4, 8, 16, and 32 Charging Stations (CSs) in each service area, respectively.
The Use of Virtual Reality in Surgical Training: Implications for Education, Patient Safety, and Global Health Equity
Authors: Laspro Matteo , Groysman Leya , Verzella Alexandra N. , Kimberly Laura L. , Flores Roberto L.
Published: November 2023

As medicine becomes more complex, there is pressure for new and more innovative educational methods. Given the economic burden associated with in-person simulation, healthcare, including the realm of surgical education, has begun employing virtual reality (VR). Potential benefits of the addition of VR to surgical learning include increased pre-operative resident exposure to surgical techniques and procedures and better patient safety outcomes. However, these new technological advances, such as VR, may not replicate organic tissues or accurately simulate medical care and surgical scenarios, creating unrealistic pseudo-environments. Similarly, while advancements have been made, there are ongoing disparities concerning the utilization of these technologies. These disparities include aspects such as the availability of stable internet connections and the cost of implementing these technologies. In accordance with other innovative technologies, VR possesses upfront economic costs that may preclude equitable use in different academic centers around the world. As such, VR may further widen educational quality between high- and low-resource nations. This analysis integrates recent innovations in VR technology with existing discourse on global health and surgical equality. In doing so, it offers preliminary guidance to ensure that the implementation of VR occurs in an equitable, safe, and sustainable fashion.
Color Sensing and Image Reconstruction Using Intelligent Machine Learning Algorithm with PINIP Radial Junction Imager
Authors: Zhang Yifei , Chen Zongsen , Zhang Shaobo , Wang Junzhuan
Published: November 2023

The development of a filterless imager has been eagerly awaited to overcome the diffraction limit when pixel sizes decrease to subwavelength scales. We propose an architecture for a filterless imager based on a symmetric inversely stacked radial junction (RJ) PINIP photodetector over silicon nanowires (SiNWs), whereby the diameter of which is less than 500 nm, which preliminarily displays the capability of bias-selected and tunable spectrum responses to the R, G, and B color bands. Assisted via suitably trained deep learning algorithms, the imager can provide more accurate color discrimination and imaging capabilities. Here, we used KNN (k-nearest neighbor) and convolution neural network (CNN) methods to retrieve the RGB ratios from the measured photocurrent value based on the pre-trained bias-tuned spectrum responses and reconstructed the images with high accuracy. Further, we demonstrated the capability of restoring sub-sampling pictures via CNN with a U-net architecture, and satisfactory reconstruction was obtained even with a sampling ratio as low as 20%. Our imaging scheme cannot only be used for high-resolution imaging but can also pave the way for application in single-pixel imaging and compressive sensing.
Group Buying Pricing Strategies of O2O Restaurants in Meituan Considering Service Levels
Authors: Dai Debao , Ma Huixian , Zhao Min , Fan Tijun
Published: November 2023

After the COVID-19 pandemic has subsided, the catering industry has returned to its previous thriving scene. Many restaurants have launched group buying services and collaborate with third-party online platforms to attract more customers. For the optimal decisions and maximum profits of restaurants and online platforms, a two-layer supply chain model is designed to investigate and study the price strategies and service strategies. Considering the service levels of restaurants and platforms, we construct the mathematical expressions of the offline retail model, online retail model, and online group buying model, respectively, and solve the game equilibrium. The results are shown as follows: (1) With cooperation between the restaurant and platform, both the restaurant and platform can obtain their maximum profits when they apply optimal pricing strategies. (2) The optimal pricing strategies of the restaurant are influenced by the sensitivity coefficients of customers to the restaurant and platform’s service levels, and the cost coefficients of the restaurant and platform’s service levels. (3) The maximum profit of the restaurant is directly proportional to the number of new potential consumers who are attracted. The more potential consumers who are attracted, the higher the profit the restaurant earns. The research results provide a reference for restaurants and platforms to formulate price strategies and service strategies, to help restaurants and platforms achieve more profits.
Selecting Goals and Target Muscles for Botulinum Toxin A Injection Using the Goal Oriented Facilitated Approach to Spasticity Treatment (GO-FAST) Tool
Authors: Jacinto Jorge , Balbert Alexander , Bensmail Djamel , Carda Stefano , Draulans Nathalie , Deltombe Thierry , Ketchum Nicholas , Molteni Franco , Reebye Rajiv
Published: November 2023

The objective of this article is to introduce the GO-FAST Tool (developed by the Toxnet group) to clinicians working in the field of neurological rehabilitation, specifically post-stroke spasticity management. The concepts utilized in the Tool and described in this article can be broadly grouped into five topics: the principles of patient-centred goal-setting; an algorithm for setting SMART (specific, measurable, attainable, realistic, and timed) treatment goals; goal-related target muscles and botulinum toxin type A dose determinants; goal attainment follow-up, scoring, and interpretation; and the multimodal approach to spasticity management. The Tool can enhance clinical practice by providing guided assistance with goal-setting and target muscle selection for botulinum toxin type A treatment. It also provides support with the follow-up evaluation of goal attainment and calculation of treatment success. The Tool is designed to be used by clinicians with varying levels of expertise in the field of neurological rehabilitation and post-stroke spasticity management, from those who are new to the field to those with many years of experience. A case study is presented in the Results Section of the article to illustrate the utility of the Tool in setting SMART treatment goals in the management of patients with post-stroke spasticity.
Combinatorial Quantum Gravity and Emergent 3D Quantum Behaviour
Authors: Trugenberger Carlo A.
Published: November 2023

We review combinatorial quantum gravity, an approach that combines Einstein’s idea of dynamical geometry with Wheeler’s “it from bit” hypothesis in a model of dynamical graphs governed by the coarse Ollivier–Ricci curvature. This drives a continuous phase transition from a random to a geometric phase due to a condensation of loops on the graph. In the 2D case, the geometric phase describes negative-curvature surfaces with two inversely related scales: an ultraviolet (UV) Planck length and an infrared (IR) radius of curvature. Below the Planck scale, the random bit character survives; chunks of random bits of the Planck size describe matter particles of excitation energy given by their excess curvature. Between the Planck length and the curvature radius, the surface is smooth, with spectral and Hausdorff dimension 2. At scales larger than the curvature radius, particles see the surface as an effective Lorentzian de Sitter surface, the spectral dimension becomes 3, and the effective slow dynamics of particles, as seen by co-moving observers, emerges as quantum mechanics in Euclidean 3D space. Since the 3D distances are inherited from the underlying 2D de Sitter surface, we obtain curved trajectories around massive particles also in 3D, representing the large-scale gravity interactions. We thus propose that this 2D model describes a generic holographic screen relevant for real quantum gravity.
Core Promoter and Pre-Core Variants of the Hepatitis B Virus (HBV) Are Frequent in Chronic Hepatitis B HBeAg-Negative Patients Infected by Genotypes A and D
Authors: Reuter Tania , Gomes-Gouvea Michele Soares , Chuffi Samira , Duque Ulisses Horst , Perini Waltesia , Azevedo Raymundo Soares , Pinho João Renato Rebello
Published: November 2023

In Brazil, hepatitis B virus endemicity is low, moderate, or high in some areas, such as Espírito Santo State in the southeast region. In this study, we intend to characterize the basal core promoter (BCP) and pre-core region (PC) variants and their association with clinical/epidemiological disease patterns in patients infected with genotypes A and D. The study included 116 chronic hepatitis B patients from Espírito Santo State, Southeast Brazil, infected with genotypes A and D. Basal core promoter (BCP) and pre-core mutations were analyzed in these patients. The frequency of BCP and PC mutations was compared with age, HBeAg status, HBV genotype and subgenotype, HBV-DNA level, clinical classification, and transmission route. HBeAg-negative status was found in 101 (87.1%) patients: 87 (75.0%) were infected with genotype A (A1 = 85; A2 = 2) and 29 (25.0%) were infected with genotype D (D3 = 24; D4 = 3; D2 = 2). BCP + PC variants altogether were more frequent (48.1%) in genotype D than in genotype A strains (6.0%) (p < 0.001). When this evaluation was performed considering the cases that presented only the A1762T and/or G1764A (BCP) mutations, it was observed that the frequency was higher in genotype A (67.5%) compared to genotype D (7.4%) (p < 0.001). On the other hand, considering the samples with mutations only in positions G1896A and/or G1899A (PC), the frequency was higher in genotype D (75.8%) than in genotype A (6.9%) (p < 0.001). Interestingly, HBV DNA was lower than 2000 IU/mL especially when both BCP/PC mutations were present (p < 0.001) or when only PC mutations were detected (p = 0.047), reinforcing their role in viral replication.
Flavonoids with Anti-Herpes Simplex Virus Properties: Deciphering Their Mechanisms in Disrupting the Viral Life Cycle
Authors: Šudomová Miroslava , Hassan Sherif T. S.
Published: November 2023

The herpes simplex virus (HSV) is a double-stranded DNA human virus that causes persistent infections with recurrent outbreaks. HSV exists in two forms: HSV-1, responsible for oral herpes, and HSV-2, primarily causing genital herpes. Both types can lead to significant complications, including neurological issues. Conventional treatment, involving acyclovir and its derivatives, faces challenges due to drug resistance. This underscores the imperative for continual research and development of new drugs, with a particular emphasis on exploring the potential of natural antivirals. Flavonoids have demonstrated promise in combating various viruses, including those within the herpesvirus family. This review, delving into recent studies, reveals the intricate mechanisms by which flavonoids decode their antiviral capabilities against HSV. By disrupting key stages of the viral life cycle, such as attachment to host cells, entry, DNA replication, latency, and reactivation, flavonoids emerge as formidable contenders in the ongoing battle against HSV infections.
SARS-CoV-2 Aerosol and Intranasal Exposure Models in Ferrets
Authors: Zumbrun Elizabeth E. , Zak Samantha E. , Lee Eric D. , Bowling Philip A. , Ruiz Sara I. , Zeng Xiankun , Koehler Jeffrey W. , Delp Korey L. , Bakken Russel R. , Hentschel Shannon S. , Bloomfield Holly A. , Ricks Keersten M. , Clements Tamara L. , Babka April M. , Dye John M. , Herbert Andrew S.
Published: November 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the worldwide COVID-19 pandemic. Animal models are extremely helpful for testing vaccines and therapeutics and for dissecting the viral and host factors that contribute to disease severity and transmissibility. Here, we report the assessment and comparison of intranasal and small particle (~3 µm) aerosol SARS-CoV-2 exposure in ferrets. The primary endpoints for analysis were clinical signs of disease, recovery of the virus in the upper respiratory tract, and the severity of damage within the respiratory tract. This work demonstrated that ferrets were productively infected with SARS-CoV-2 following either intranasal or small particle aerosol exposure. SARS-CoV-2 infection of ferrets resulted in an asymptomatic disease course following either intranasal or small particle aerosol exposure, with no clinical signs, significant weight loss, or fever. In both aerosol and intranasal ferret models, SARS-CoV-2 replication, viral genomes, and viral antigens were detected within the upper respiratory tract, with little to no viral material detected in the lungs. The ferrets exhibited a specific IgG immune response to the SARS-CoV-2 full spike protein. Mild pathological findings included inflammation, necrosis, and edema within nasal turbinates, which correlated to positive immunohistochemical staining for the SARS-CoV-2 virus. Environmental sampling was performed following intranasal exposure of ferrets, and SARS-CoV-2 genomic material was detected on the feeders and nesting areas from days 2–10 post-exposure. We conclude that both intranasal and small particle aerosol ferret models displayed measurable parameters that could be utilized for future studies, including transmission studies and testing SARS-CoV-2 vaccines and therapeutics.
Bats—The Magnificent Virus Player: SARS, MERS, COVID-19 and Beyond
Authors: Li Kenneth S. M. , Lau Susanna K. P. , Woo Patrick C. Y.
Published: November 2023

Irrespective of whether COVID-19 originated from a natural or a genetically engineered virus, the ultimate source of Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) is bats [...]
Processing of the 3C/D Region of the Deformed Wing Virus (DWV)
Authors: Reuscher Carina Maria , Barth Sandra , Gockel Fiona , Netsch Anette , Seitz Kerstin , Rümenapf Till , Lamp Benjamin
Published: November 2023

The deformed wing virus (DWV) belongs to the genus Iflavirus and the family Iflaviridae within the order Picornavirales. It is an important pathogen of the Western honey bee, Apis mellifera, causing major losses among honey bee colonies in association with the ectoparasitic mite Varroa destructor. Although DWV is one of the best-studied insect viruses, the mechanisms of viral replication and polyprotein processing have been poorly studied in the past. We investigated the processing of the protease-polymerase region at the C-terminus of the polyprotein in more detail using recombinant expression, novel serological reagents, and virus clone mutagenesis. Edman degradation of purified maturated polypeptides uncovered the C- and N-termini of the mature 3C-like (3CL) protease and RNA-dependent RNA polymerase (3DL, RdRp), respectively. Autocatalytic processing of the recombinant DWV 3CL protease occurred at P1 Q2118 and P1′ G2119 (KPQ/GST) as well as P1 Q2393 and P1′ S2394 (HAQ/SPS) cleavage sites. New monoclonal antibodies (Mab) detected the mature 3CL protease with an apparent molecular mass of 32 kDa, mature 3DL with an apparent molecular mass of 55 kDa as well as a dominant 3CDL precursor of 90 kDa in DWV infected honey bee pupae. The observed pattern corresponds well to data obtained via recombinant expression and N-terminal sequencing. Finally, we were able to show that 3CL protease activity and availability of the specific protease cleavage sites are essential for viral replication, protein synthesis, and establishment of infection using our molecular clone of DWV-A.
Predictors of Motivation to Receive a COVID-19 Vaccination and the Number of COVID-19 Vaccine Doses Received in Patients with Schizophrenia
Authors: Lin Chien-Wen , Chang Yu-Ping , Yen Cheng-Fang
Published: November 2023

Individuals with schizophrenia are more likely to be infected with COVID-19 than are members of the general population. No prospective study has examined the associations of multi-dimensional factors with the motivation to receive vaccination against COVID-19. This follow-up study investigated the effects of individual (sociodemographic and illness characteristics, depression, and self-esteem), environmental (perceived social support), and individual–environmental interaction factors (self-stigma and loneliness) on the motivation to receive vaccination against COVID-19 and the number of COVID-19 vaccine doses received one year later among 300 individuals with schizophrenia. The associations of baseline factors with motivation to receive vaccination against COVID-19 and the number of vaccine doses received 1 year later were examined through linear regression analysis. The results indicated that greater loneliness (p < 0.01) and being married or cohabitating (p < 0.05) at baseline were significantly associated with lower motivation to receive vaccination against COVID-19 at follow-up. Disorganization (p < 0.05) at baseline was significantly associated with fewer COVID-19 vaccine doses at follow-up; greater motivation to receive vaccination was significantly associated with more COVID-19 vaccine doses at follow-up (p < 0.001). Health professionals should consider the identified predictors while developing intervention programs aimed at enhancing vaccination against COVID-19 in individuals with schizophrenia.
Trends in Vaccine Completeness in Children Aged 0–23 Months in Cape Town, South Africa
Authors: Ndwandwe Duduzile , Ndlovu Musawenkosi , Mayeye Asanda , Luphondo Nomahlubi , Muvhulawa Ndivhuwo , Ntamo Yonela , Dludla Phiwayinkosi V. , Wiysonge Charles S.
Published: November 2023

Background: We have previously determined that the occurrence of missed vaccination opportunities in children in Cape Town, South Africa, is shaped by both individual and contextual factors. These factors present valuable openings for enhancing quality and implementing broader strategies to enhance the delivery of routine Immunisation services. Methods: Here, we are further reporting regional-level data on the coverage and factors influencing vaccination completion within a similar study population, based on extensive data analysis from the 2016 South African Demographic and Health Survey. Results and discussion: The study reveals commendable vaccination coverage for most vaccines within recommended schedules, with high rates of initial vaccinations at birth and during the primary vaccination schedule. However, there are notable areas for improvement, particularly in ensuring complete coverage for the second measles vaccine and the 18-month vaccine. Socio-demographic factors also play a role, with maternal education and caregiver awareness campaigns showing the potential to positively influence vaccination completeness. This study emphasises the importance of timely vaccinations during the early months of life and underscores the need for interventions to maintain coverage as children age. Specific sub-districts, such as Tygerberg, may require targeted efforts to enhance vaccination completeness. Additionally, assessing caregiver knowledge about child vaccination is deemed vital, as it can impact vaccination decisions and adherence. Conclusions: The findings provide valuable insights for public health interventions in Cape Town, aimed at reducing the burden of vaccine-preventable diseases and ensuring the health of the region’s youngest population.
Cancer Vaccines in the Immunotherapy Era: Promise and Potential
Authors: Verma Chaitenya , Pawar Vishakha , Srivastava Shivani , Tyagi Anuradha , Kaushik Gaurav , Shukla Surendra , Kumar Vinay
Published: November 2023

Therapeutic vaccines are a promising alternative for active immunotherapy for different types of cancers. Therapeutic cancer vaccines aim to prevent immune system responses that are not targeted at the tumors only, but also boost the anti-tumor immunity and promote regression or eradication of the malignancy without, or with minimal, adverse events. Clinical trial data have pushed the development of cancer vaccines forward, and the US Food and Drug Administration authorized the first therapeutic cancer vaccine. In the present review, we discuss the various types of cancer vaccines and different approaches for the development of therapeutic cancer vaccines, along with the current state of knowledge and future prospects. We also discuss how tumor-induced immune suppression limits the effectiveness of therapeutic vaccinations, and strategies to overcome this barrier to design efficacious, long-lasting anti-tumor immune responses in the generation of vaccines.
Seepage Characteristics of Shale Fracture and the Effect of Filling Sand under Normal Stress
Authors: Gong Yan , Xie Xinghua , Liu Jingkai
Published: November 2023

As a new type of unconventional natural gas resource, shale gas plays a vital role in energy supply. In order to deeply understand the shale fracture seepage characteristics, filled and unfilled fracture seepage tests were carried out on shale samples with different fracture surface topography with the use of self-developed shale fracture seepage testing equipment. The fitting formula that the seepage discharge decreased as a negative exponential function with the increase in normal stress was obtained in the unfilled fracture seepage test; the fitting coefficient had a good logarithmic and exponential function relationship with joint roughness coefficient JRC and surface development interface area ratio Sdr. Meanwhile, the modified cubic law was obtained by adding the correction coefficient. The correction coefficient had an exponential relationship with the anisotropy value Ka. Compared with the unfilled one, the fracture surface topography had little effect on the seepage discharge when it was filled. The experimental results show the effects of water head difference, fracture surface topography, particle size, and thickness of filling sand on shale fracture seepage characteristics under different normal stress conditions, which have a certain significance for improving the efficiency of shale gas production.
Assessment of Stoplog Gates’ Operational Effectiveness for Improving Discharged-Water Temperatures during the Thermal Stratification Period in a Reservoir
Authors: Liu Lijin , Tuo Youcai , Xia Hao , Deng Yun , Zhang Xu , Wang Haoyu
Published: November 2023

The discharge of low-temperature water from the middle and lower layers of thermally stratified dam reservoirs leads to thermal pollution and adversely affects fish production and reproduction in downstream rivers. Selective water withdrawal using stoplog gates is an effective approach to address this issue. However, comprehensively and effectively evaluating the effects of stratified withdrawal and optimizing the scheduling of stoplog gates to provide better ecological services to downstream habitats pose significant challenges for reservoir managers. In this study, an equivalent elevation method (EEM) was developed based on in situ observation data of water temperature. The EEM calculates the equivalent withdrawal elevation (EWE) in the far dam area corresponding to the discharge-water temperature (DWT), facilitating the evaluation of stoplog-gate effects. EEM was applied to a thermally stratified dam reservoir in southwestern China (Guangzhao reservoir, GZ). The results showed a significant positive correlation (r > 0.7, p < 0.05) between DWT and the vertical-water-temperature structure in the far dam area. The average EWEs for the 5-layer, 4-layer, and 3-layer stoplog gates in GZ were 697.2 m, 690.8 m, and 689.9 m, respectively. Utilizing the EEM findings, a scheduling operation scheme was proposed to improve DWT while reducing the number of stoplog-gate operations, thereby increasing their efficiency and saving the cost of invested labor and time. This method provides a reference for reservoir managers in optimizing stoplog-gate-scheduling strategies to mitigate potential risks to aquatic ecosystems caused by abnormal water temperature.
Organochlorine Pesticides in Soil–Groundwater–Plant System in a Famous Agricultural Production Area in China: Spatial Distribution, Source Identification and Migration Prediction
Authors: Li Chuansheng , Qi Xiaofan , Wang Yu , Meng Qingjie , Li Wenpeng , Liu Lanyu , Zheng Yuejun , Cui Huqun
Published: November 2023

Being a famous hometown of vegetables in China, Shouguang City has a long history of vegetable cultivation and is a comprehensive national commodity base for vegetables and fruits. In recent decades, with the expansion of agricultural production, the use of pesticides is increasing. Although organochlorine pesticides (OCPs) have been banned, there are still some residues in soil, groundwater and other media. The study on the migration of the OCPs in soil and groundwater is of great importance for the maintenance of food security. Using methods of hydrogeological survey, laboratory testing and numerical simulation, the spatial distribution characteristics, sources and pollution degree of OCPs in soil and groundwater were analyzed, and the migration and transformation of OCPs in soil–groundwater was dynamically simulated and predicted. The study showed that there were many kinds of OCPs residual in the soil environment. The hexachlorocyclohexane (HCHs) in the topsoil of the study area were mainly due to the use of lindane, and the main source of dichlorodiphenyltrichloroethane (DDTs) in soil was the use of DDTs in history. The contents of HCHs, DDTs and hexachlorobenzene in the topsoil of the study area were at a low level, while the content of endosulfan metabolite endosulfan sulfate was comparatively higher. In recent years, the content of organochlorine pesticides in soil has generally decreased. The indexes of OCPs in groundwater can meet the Standards for Drinking Water quality of China. It was predicted that the HCH pollutants would mainly be distributed in Gucheng Street, Shangkou Town, and its south area where the concentration exceeds 3 ng/L exceeds that of 240 km2. DDT pollutants would mainly be distributed in the east and north of Shouguang City, where the concentration beyond 0.6 ng/L exceeds that of 200 km2. Endosulfan sulfate pollutants would mainly be distributed in the Gucheng Street Office, Shangkou Town, and its south area where the concentration exceeds 1.5 ng/L will exceed that of 150 km2. Hexachlorobenzene (HCB) pollutants would mainly be distributed in Fengcheng in the west of Shouguang City and Nancha River in the northeast, where the concentration exceeds 0.2 ng/L will exceed that of 200 km2. The study enriched the monitoring data of OCPs in agricultural planting areas and provided reference for source analysis, migration prediction, and pollution prevention of OCPs.
Evaluation of Infiltration Modeling in the Cisadane Watershed in Indonesia: Existing and New Approach Equation
Authors: Hidayat Dina P. A. , Darsono Sri Legowo W. , Farid Mohammad
Published: November 2023

This study aims to evaluate the accuracy of semi-empirical models for determining infiltration based on the existing equations (Horton, Kostiakov and Kostiakov–Lewis) and a new form equation with optimized parameters which has good accuracy (lowest RMSE and MBE), sufficient linearity (highest R2), and a Willmott index close to 1. The location of this study was the Cisadane watershed, which is a priority watershed in Indonesia. The lowest values of average RMSE and MBE and highest values of R2 and Willmott index are given by Equation 1, which indicated that the model was closest to the field infiltration conditions. The distribution of the RMSE values for all survey points using Equation 1 resulted in small RMSE values ranging from 0.09 to 1.83. The distribution of R2 values for all survey points using Equation 1 yielded R2 values close to 1 (high linearity) with values ranging from 0.96 to 1. Based on these results, it can be concluded that Equation 1 is the most accurate infiltration model for the Cisadane watershed compared to the existing infiltration rate equation. This research can be used as an initial idea to create the infiltration rate model using other forms of equations with optimization parameters to produce a more representative model with field data.
Occurrence and Sources of Polycyclic Aromatic Hydrocarbons and Factors Influencing Their Accumulation in Surface Sediment of a Deep-Sea Depression, Namely, the Tatar Trough (Tatar Strait, the Sea of Japan)
Authors: Koudryashova Yuliya , Chizhova Tatiana , Zadorozhny Pavel , Ponomareva Anna , Eskova Alena
Published: November 2023

The concentrations of 14 polycyclic aromatic hydrocarbons (PAHs) in the sediment of the Tatar Trough were studied. Despite the increase in PAH concentrations over recent decades, which is likely the result of the handling and transportation of fossil fuels, PAH levels and ecological risk were found to be low. The spatial pattern revealed that higher PAH concentrations were mainly in the deeper water sites, suggesting that trough slope failure transported the PAHs to the deeper part of the basin. There was no correlation between the PAHs and grain size or the PAHs and organic carbon that is related to the PAH input from a variety of sources and the heterogeneity of organic matter. The PAH composition, isomer ratio, and PCA identified two areas with different PAH sources. The most northern part of the Tatar Trough received petrogenic PAHs that are probably transported downslope from the northern Tatar Strait where fossil fuels are handled in some ports. Another trough part was polluted by the PAHs from the combustion of coal and biomass and the exhaust of marine vehicles. The minor presence of genes responsible for aerobic PAH destruction can be explained by the anaerobic degradation of PAHs or the spontaneous creation of favorable conditions that promote bacterial PAH oxidation.
Impact of V2G Flexibility on Congestion Management in the German Transmission Grid
Authors: Ruppert Manuel , Baumgartner Nora , Märtz Alexandra , Signer Tim
Published: November 2023

In this study, we investigate the effect of vehicle-to-grid (V2G) flexibility potential on solving transmission grid congestion in Germany using congestion management measures. We extend existing work on effects of V2G on transmission grid congestion by determining the flexibility provided for improving grid operation based on mobility behavior and findings on V2G user requirements from real-world electric vehicle users. Furthermore, the impact on transmission grid operation is analyzed using an optimal congestion management model with high temporal and spatial resolution. Using a scenario for the year 2030 with ambitious targets for European renewable generation development and electrification of private vehicles, our findings show that by enabling the available fleet of V2G vehicles to participate in congestion management, cost and amount can be reduced by up to 11%. However, the required capacity is shown to be lower than installed capacities in ambitious future scenarios, implying that a limited number of vehicles close to congestion centers will be utilized for transmission grid operation. Our results further suggest that high numbers of vehicles with low availability of V2G for grid operation purposes can lead to an increase in congestion management measures, while V2G proves beneficial for congestion management emissions and cost in all scenarios.
Changing Contours of Strategies in Complexity
Authors: Breed Ms. Shloka , Gupte Rajiv
Published: November 2023

In this complex, interconnected world, we are already witnessing “Lorez effect” in most spheres of life. Sectoral approaches rely upon actor's behavior including resource- based models, and politic economic outcomes (Kurth 1979, Rogowski, 1989) (Gourevitch 1986) (Glimore 1997). Their approaches explain technological changes in globalized world in sectoral variation, without referring to institutionalizing innovations. According to Freaman & Perez, “Technology has wide spread consequences. In that, the diffusion is accompanied by structural crisis of adjustment