bluuebunny/crossref_metadata_2025 huggingface dataset:
Geoparks and Education: UNESCO Global Geopark Villuercas-Ibores-Jara as a Case Study in Spain
Authors: Fernández Álvarez Rubén
Published: January 2020

The UNESCO Global Geoparks is a good didactic instrument that allows teaching territorial elements in transversal way. The geopark provides a means to address the teaching-learning process of both cultural values and natural values, including geology. This paper aims to highlight the educational activities that have emerged from the Villuercas UNESCO Global Geopark. For this purpose, a qualitative methodology based on document analysis and fieldwork has been used. Through a system of categories, we have analyzed the existing materials on the geopark’s website, its activity book and the schools that have been part of its educational project. The geopark and educational centers collaboration has 76 educational proposals that are developed in the territory of the geopark. Since 2011, all schools in its area of action participate in its educational project with a total of 2500 students per year. The Villuercas geopark involves all students in its territory and encourages learning of a significant, transversal and collaborative type. In the same way, it has a positive impact on environmental awareness and respect for the environment and cultural resources.
Applications of Next Generation Sequencing to the Analysis of Familial Breast/Ovarian Cancer
Authors: Zelli Veronica , Compagnoni Chiara , Cannita Katia , Capelli Roberta , Capalbo Carlo , Di Vito Nolfi Mauro , Alesse Edoardo , Zazzeroni Francesca , Tessitore Alessandra
Published: January 2020

Next generation sequencing (NGS) provides a powerful tool in the field of medical genetics, allowing one to perform multi-gene analysis and to sequence entire exomes (WES), transcriptomes or genomes (WGS). The generated high-throughput data are particularly suitable for enhancing the understanding of the genetic bases of complex, multi-gene diseases, such as cancer. Among the various types of tumors, those with a familial predisposition are of great interest for the isolation of novel genes or gene variants, detectable at the germline level and involved in cancer pathogenesis. The identification of novel genetic factors would have great translational value, helping clinicians in defining risk and prevention strategies. In this regard, it is known that the majority of breast/ovarian cases with familial predisposition, lacking variants in the highly penetrant BRCA1 and BRCA2 genes (non-BRCA), remains unexplained, although several less penetrant genes (e.g., ATM, PALB2) have been identified. In this scenario, NGS technologies offer a powerful tool for the discovery of novel factors involved in familial breast/ovarian cancer. In this review, we summarize and discuss the state of the art applications of NGS gene panels, WES and WGS in the context of familial breast/ovarian cancer.
Investigation of Incidents and Trends of Antimicrobial Resistance in Foodborne Pathogens in Eight Countries from Historical Sample Data
Authors: Yang Katherine , Wang Annie , Fu Matthew , Wang Aaron , Chen Kevin , Jia Qian , Huang Zuyi
Published: January 2020

Antimicrobial resistance (AMR) causes millions of illnesses every year, threatening the success of lifesaving antibiotic therapy and, thus, public health. To examine the rise and spread of antimicrobial resistance around the world, our study performs a multivariate statistical analysis of antimicrobial resistance gene data from eight different countries: the US, the UK, China, Brazil, Mexico, Canada, Australia, and South Africa. Multi-dimensional data points were projected onto a two-dimensional plane using principal component analysis and organized into a dendrogram utilizing hierarchical clustering to identify significant AMR genes and pathogens. Outlier genes/pathogens were typically involved in high occurrences of antimicrobial resistance, and they were able to indicate the trend of antimicrobial resistance in the future. Statistical analysis of the data identified: (1) tet(A), aph(3″)-Ib, aph(6)-Id, blaEC, blaTEM-1, qacEdelta1, sul1, sul2, and aadA1 as the nine most common AMR genes among the studied countries; (2) Salmonella enterica and E. coli and Shigella as the most common AMR foodborne pathogens; and (3) chicken as the most prevalent meat carrier of antimicrobial resistance. Our study shows that the overall number of reported antimicrobial resistance cases in foodborne pathogens is generally rising. One potential contributing factor for this is the increasing antimicrobial usage in the growing livestock industry.
The Effect of Psychological First Aid Training on Knowledge and Understanding about Psychosocial Support Principles: A Cluster-Randomized Controlled Trial
Authors: Sijbrandij Marit , Horn Rebecca , Esliker Rebecca , O’May Fiona , Reiffers Relinde , Ruttenberg Leontien , Stam Kimberly , de Jong Joop , Ager Alastair
Published: January 2020

Psychological first aid (PFA) is a world-wide implemented approach to helping people affected by an emergency, disaster, or other adverse event. Controlled evaluations of PFA’s training effects are lacking. We evaluated the effectiveness of a one-day PFA training on the acquisition and retention of knowledge of appropriate responses and skills in the acute aftermath of adversity in Peripheral Health Units (PHUs) in post-Ebola Sierra Leone. Secondary outcomes were professional quality of life, confidence in supporting a distressed person, and professional attitude. PHUs in Sierra Leone (n = 129) were cluster-randomized across PFA (206 participants) and control (202 participants) in March 2017. Knowledge and understanding of psychosocial support principles and skills were measured with a questionnaire and two patient scenarios to which participants described helpful responses. Professional attitude, confidence, and professional quality of life were assessed using self-report instruments. Assessments took place at baseline and at three- and six-months post-baseline. The PFA group had a stronger increase in PFA knowledge and understanding at the post-PFA training assessment (d = 0.50; p < 0.001) and at follow-up (d = 0.43; p = 0.001). In addition, the PFA group showed better responses to the scenarios at six-months follow-up (d = 0.38; p = 0.0002) but not at the post-assessment (d = 0.04; p = 0.26). No overall significant differences were found for professional attitude, confidence, and professional quality of life. In conclusion, PFA training improved acquisition and retention of knowledge and understanding of appropriate psychosocial responses and skills in providing support to individuals exposed to acute adversity. Our data support the use of PFA trainings to strengthen capacity for psychosocial support in contexts of disaster and humanitarian crisis. Future studies should examine the effects of PFA on psychosocial outcomes for people affected by crises.
Citizen Science-Informed Community Master Planning: Land Use and Built Environment Changes to Increase Flood Resilience and Decrease Contaminant Exposure
Authors: Newman Galen , Shi Tianqi , Yao Zhen , Li Dongying , Sansom Garett , Kirsch Katie , Casillas Gaston , Horney Jennifer
Published: January 2020

Communities adjacent to concentrated areas of industrial land use (CAILU) are exposed to elevated levels of pollutants during flood disasters. Many CAILU are also characterized by insufficient infrastructure, poor environmental quality, and socially vulnerable populations. Manchester, TX is a marginalized CAILU neighborhood proximate to several petrochemical industrial sites that is prone to frequent flooding. Pollutants from stormwater runoff discharge from industrial land uses into residential areas have created increased toxicant exposures. Working with local organizations, centers/institutes, stakeholders, and residents, public health researchers sampled air, water, indoor dust, and outdoor soil while researchers from landscape architecture and urban planning applied these findings to develop a community-scaled master plan. The plan utilizes land use and built environment changes to increase flood resiliency and decrease exposure to contaminants. Using a combination of models to assess the performance, costs, and benefits of green infrastructure and pollutant load impacts, the master plan is projected to capture 147,456 cubic feet of runoff, and create $331,400 of annual green benefits by reducing air pollution and energy use, providing pollution treatment, increase carbon dioxide sequestration, and improve groundwater replenishment. Simultaneously, there is a 41% decrease across all analyzed pollutants, reducing exposure to and transferal of toxic materials.
The Assessment of Problematic Internet Pornography Use: A Comparison of Three Scales with Mixed Methods
Authors: Chen Lijun , Jiang Xiaoliu
Published: January 2020

The primary aim of this study was to compare different screening tools for problematic internet pornography use (IPU) and identify the most accurate measure. The reliability and validity of three scales, namely, the Problematic Pornography Consumption Scale (PPCS), Problematic Pornography Use Scale (PPUS), and Short Internet Addiction Test Adapted to Online Sexual Activities(s-IAT-sex), were examined using three homogeneous groups, respectively. A total of 972 adults (mean age = 24.8) from 28 provinces/regions in China participated in the quantitative part (QUAN). The Brief Pornography Screener served as the reference standard. The PPCS demonstrated stronger reliability and validity, including criterion validity, as well as greater sensitivity and acceptable specificity; therefore, it was considered to be the more accurate screening instrument. In the qualitative part (QUAL), we interviewed 22 volunteers and 11 therapists (who had worked with individuals with problematic IPU) to examine their perspectives on the core features of problematic IPU and dimensions of the PPCS. Almost all the interviewees endorsed the structure of the PPCS. These findings encourage the use of the PPCS in future research studies and underscore its screening applications because of its ability to classify IPU as problematic or nonproblematic.
Artificial Intelligence-Enhanced Decision Support for Informing Global Sustainable Development: A Human-Centric AI-Thinking Approach
Authors: How Meng-Leong , Cheah Sin-Mei , Chan Yong-Jiet , Khor Aik Cheow , Say Eunice Mei Ping
Published: January 2020

Sustainable development is crucial to humanity. Utilization of primary socio-environmental data for analysis is essential for informing decision making by policy makers about sustainability in development. Artificial intelligence (AI)-based approaches are useful for analyzing data. However, it was not easy for people who are not trained in computer science to use AI. The significance and novelty of this paper is that it shows how the use of AI can be democratized via a user-friendly human-centric probabilistic reasoning approach. Using this approach, analysts who are not computer scientists can also use AI to analyze sustainability-related EPI data. Further, this human-centric probabilistic reasoning approach can also be used as cognitive scaffolding to educe AI-Thinking in the analysts to ask more questions and provide decision making support to inform policy making in sustainable development. This paper uses the 2018 Environmental Performance Index (EPI) data from 180 countries which includes performance indicators covering environmental health and ecosystem vitality. AI-based predictive modeling techniques are applied on 2018 EPI data to reveal the hidden tensions between the two fundamental dimensions of sustainable development: (1) environmental health; which improves with economic growth and increasing affluence; and (2) ecosystem vitality, which worsens due to industrialization and urbanization.
Antiviral Effectors and Gene Drive Strategies for Mosquito Population Suppression or Replacement to Mitigate Arbovirus Transmission by Aedes aegypti
Authors: Williams Adeline , Franz Alexander , Reid William , Olson Ken
Published: January 2020

The mosquito vector Aedes aegypti transmits arthropod-borne viruses (arboviruses) of medical importance, including Zika, dengue, and yellow fever viruses. Controlling mosquito populations remains the method of choice to prevent disease transmission. Novel mosquito control strategies based on genetically manipulating mosquitoes are being developed as additional tools to combat arbovirus transmission. Genetic control of mosquitoes includes two basic strategies: population suppression and population replacement. The former aims to eliminate mosquito populations while the latter aims to replace wild populations with engineered, pathogen-resistant mosquitoes. In this review, we outline suppression strategies being applied in the field, as well as current antiviral effector genes that have been characterized and expressed in transgenic Ae. aegypti for population replacement. We discuss cutting-edge gene drive technologies that can be used to enhance the inheritance of effector genes, while highlighting the challenges and opportunities associated with gene drives. Finally, we present currently available models that can estimate mosquito release numbers and time to transgene fixation for several gene drive systems. Based on the recent advances in genetic engineering, we anticipate that antiviral transgenic Ae. aegypti exhibiting gene drive will soon emerge; however, close monitoring in simulated field conditions will be required to demonstrate the efficacy and utility of such transgenic mosquitoes.
Nasal Nitric Oxide in Chronic Rhinosinusitis with or without Nasal Polyps: A Systematic Review with Meta-Analysis
Authors: Ambrosino Pasquale , Molino Antonio , Spedicato Giorgio Alfredo , Parrella Paolo , Formisano Roberto , Motta Andrea , Di Minno Matteo Nicola Dario , Maniscalco Mauro
Published: January 2020

Background and Aims: There has been a recent growing interest in the role of nasal nitric oxide (nNO) as a biomarker for osteomeatal complex obstruction in paranasal sinus diseases. By using meta-analysis, we systematically reviewed the literature to establish the possible link between nNO concentration and chronic rhinosinusitis with nasal polyps (CRSwNP) or without (CRSsNP). Methods: We systematically searched the EMBASE, PubMed, Scopus, and Web of Science databases for related studies. Differences between controls and cases were reported as standardized mean difference (SMD), with 95% confidence intervals (95% CI), using the random-effects method. Results: We selected 23 articles for the final analysis: 15 with data on 461 CRSwNP patients and 384 healthy controls, 10 with data on 183 CRSsNP patients and 260 controls, and 14 studies on 372 CRSwNP and 297 CRSsNP patients. CRSwNP patients showed significantly lower nNO values when compared to both healthy controls (SMD: −1.495; 95% CI: −2.135, −0.854; p < 0.0001) and CRSsNP patients (SMD: −1.448; 95% CI: −2.046, −0.850; p < 0.0001). Sensitivity and subgroup analyses confirmed the results, which were further refined by regression models. They showed that an increasing aspiration flow is related to a greater difference in nNO levels between cases and control subjects. We also documented lower nNO levels in CRSsNP patients with respect to controls (SMD: −0.696; 95% CI: −1.189, −0.202; p = 0.006), being this result no longer significant when excluding patients in therapy with intranasal corticosteroids. As shown by regression models, the increased Lund–Mackay score indicates a high effect size. Conclusions: nNO levels are significantly lower in CRSwNP, especially when using higher aspiration flows. Additional studies are needed to define one single standardized method and normal reference values for nNO.
High-Temperature Diffusion Bonding of Ti–6Al–4V and Super-Duplex Stainless Steel Using a Cu Interlayer Embedded with Alumina Nanoparticles
Authors: Cooke Kavian O. , Richardson Anthony , Khan Tahir I. , Shar Muhammad Ali
Published: January 2020

In this study, Ti–6Al–4V alloy was diffusion bonded to super-duplex stainless steel (SDSS) using an electrodeposited Cu interlayer containing alumina nanoparticles to determine the effects of bonding parameters on the microstructural evolution within the joint region. The results of the study showed that the homogeneity of the joint is affected by the bonding time and bonding temperature. The results also showed that when a Cu/Al2O3 interlayer is used, Ti–6Al–4V alloy can be successfully diffusion bonded to SDSS at temperatures above 850 °C. The combination of longer bonding time and high bonding temperature leads to the formation of various intermetallic compounds within the interface. However, the presence of the Al2O3 nanoparticles within the interface causes a change in the volume, size, and shape of the intermetallic compounds formed by pinning grain boundaries and restricting grain growth of the interlayer. The variation of the chemical composition and hardness across the bond interface confirmed a better distribution of hard phases within the joint center when a Cu/Al2O3 interlayer was used.
The Equilibrium Scour Depth around a Pier under the Action of Collinear Waves and Current
Authors: Gazi Ainal Hoque , Purkayastha Subhrangshu , Afzal Mohammad Saud
Published: January 2020

In this paper, a mathematical equation is developed for the equilibrium scour depth considering an arbitrary shape of the scour hole around a pier under the action of collinear waves and current. A power-law current velocity profile is assumed for the purpose of the analysis. The equilibrium scour depth is obtained by equating the work done by the flowing fluid while interacting with the pier under the action of the collinear waves and the current and the work done by the total volume of the sediment particles removed from the scour hole, respectively. The equilibrium scour depths predicted by the model show good agreement with the experimental and numerical results available in the literature.
Analysis of the Effectiveness of the Step Vacuum Preloading Method: A Case Study on High Clay Content Dredger Fill in Tianjin, China
Authors: Li Jinfeng , Chen Huie , Yuan Xiaoqing , Shan Wenchong
Published: January 2020

As a solution to avoid the blockage of the drainage pipe by traditional vacuum preloading, step vacuum preloading (SVP) has been progressively studied. However, the effectiveness of this technique has yet to be systematically analyzed. In this study, an indoor model test was conducted in which vacuum pressure was applied in five stages (10, 20, 40, 60, and 80 kPa) to dredger soil with high clay content at a reclamation site in Binhai New Area, Tianjin, China. The extent of the consolidation effect of the soil was determined, and the effectiveness of the step vacuum preloading method to address drainage pipe blockage was evaluated. The results indicate that soil settlement increases at each stage of vacuum pressure treatment and the degree of vertical consolidation at each stage exceeds 90%. At the end of the treatment stage with vacuum pressure of 80 kPa, the weakly bound water was discharged. Dissipation of pore water pressure occurred in all stages. On the basis of these results, it is shown that SVP can efficiently reinforce dredger fill. Moreover, after SVP, the grain size of the soil and void ratio are still uniformly distributed. Regardless of their location from the drainage pipe, soil exhibits permeability coefficients within the same order of magnitude. The consolidation effect of soil in each stage and the increased drainage rate in the initial stage of vacuum preloading with 80 kPa indicate that the test in the current study can decrease the horizontal displacement of fine particles and can avoid drainage pipe blockage.
Detecting Azole-Antifungal Resistance in Aspergillus fumigatus by Pyrosequencing
Authors: van der Torre Mireille H. , Novak-Frazer Lilyann , Rautemaa-Richardson Riina
Published: January 2020

Guidelines on the diagnosis and management of Aspergillus disease recommend a multi-test approach including CT scans, culture, fungal biomarker tests, microscopy and fungal PCR. The first-line treatment of confirmed invasive aspergillosis (IA) consists of drugs in the azole family; however, the emergence of azole-resistant isolates has negatively impacted the management of IA. Failure to detect azole-resistance dramatically increases the mortality rates of azole-treated patients. Despite drug susceptibility tests not being routinely performed currently, we suggest including resistance testing whilst diagnosing Aspergillus disease. Multiple tools, including DNA sequencing, are available to screen for drug-resistant Aspergillus in clinical samples. This is particularly beneficial as a large proportion of IA samples are culture negative, consequently impeding susceptibility testing through conventional methods. Pyrosequencing is a promising in-house DNA sequencing method that can rapidly screen for genetic hotspots associated with antifungal resistance. Pyrosequencing outperforms other susceptibility testing methods due to its fast turnaround time, accurate detection of polymorphisms within critical genes, including simultaneous detection of wild type and mutated sequences, and—most importantly—it is not limited to specific genes nor fungal species. Here we review current diagnostic methods and highlight the potential of pyrosequencing to aid in a diagnosis complete with a resistance profile to improve clinical outcomes.
High-Performance Lithium-Rich Layered Oxide Material: Effects of Preparation Methods on Microstructure and Electrochemical Properties
Authors: Liu Qiming , Zhu Huali , Liu Jun , Liao Xiongwei , Tang Zhuolin , Zhou Cankai , Yuan Mengming , Duan Junfei , Li Lingjun , Chen Zhaoyong
Published: January 2020

Lithium-rich layered oxide is one of the most promising candidates for the next-generation cathode materials of high-energy-density lithium ion batteries because of its high discharge capacity. However, it has the disadvantages of uneven composition, voltage decay, and poor rate capacity, which are closely related to the preparation method. Here, 0.5Li2MnO3·0.5LiMn0.8Ni0.1Co0.1O2 was successfully prepared by sol–gel and oxalate co-precipitation methods. A systematic analysis of the materials shows that the 0.5Li2MnO3·0.5LiMn0.8Ni0.1Co0.1O2 prepared by the oxalic acid co-precipitation method had the most stable layered structure and the best electrochemical performance. The initial discharge specific capacity was 261.6 mAh·g−1 at 0.05 C, and the discharge specific capacity was 138 mAh·g−1 at 5 C. The voltage decay was only 210 mV, and the capacity retention was 94.2% after 100 cycles at 1 C. The suppression of voltage decay can be attributed to the high nickel content and uniform element distribution. In addition, tightly packed porous spheres help to reduce lithium ion diffusion energy and improve the stability of the layered structure, thereby improving cycle stability and rate capacity. This conclusion provides a reference for designing high-energy-density lithium-ion batteries.
Preparation and Corrosion Behavior in Marine Environment of MAO Coatings on Magnesium Alloy
Authors: Yao Yuhong , Yang Wei , Liu Dongjie , Gao Wei , Chen Jian
Published: January 2020

To improve the corrosion performance of magnesium alloys in the marine environment, the MAO, MAO–Cu2CO3(OH)2·H2O and MAO–Cu2P2O7 ceramic coatings were deposited on AZ91D magnesium alloys in basic electrolyte and the discoloration mechanism of the Cu-doped MAO coatings and the corrosion behavior of the three MAO coatings in the artificial seawater solution were investigated by SEM, EDS and XPS. The results indicated that the formation and discoloration mechanism of the brown MAO ceramic coatings were attributable to the formation of Cu2O in the coatings. Though the three MAO coatings had a certain protective effect against the corrosion of AZ91D substrate in the artificial seawater, the distinctive stratification phenomenon was found on the MAO–Cu2P2O7 coated sample and the corrosion model of the MAO–Cu2P2O7 coatings in the immersion experiment was established. Therefore, the brown Cu-doped MAO coatings were speculated to significantly reduce the risk of the magnesium parts in marine environments.
Freeze-FRESH: A 3D Printing Technique to Produce Biomaterial Scaffolds with Hierarchical Porosity
Authors: Wang Zi , Florczyk Stephen J.
Published: January 2020

Tissues are organized in hierarchical structures comprised of nanoscale, microscale, and macroscale features. Incorporating hierarchical structures into biomaterial scaffolds may enable better resemblance of native tissue structures and improve cell interaction, but it is challenging to produce such scaffolds using a single conventional scaffold production technique. We developed the Freeze-FRESH (FF) technique that combines FRESH 3D printing (3DP) and freeze-casting to produce 3D printed scaffolds with microscale pores in the struts. FF scaffolds were produced by extrusion 3DP using a support bath at room temperature, followed by freezing and lyophilization, then the FF scaffolds were recovered from the bath and crosslinked. The FF scaffolds had a hierarchical pore structure from the combination of microscale pores throughout the scaffold struts and macroscale pores in the printed design, while control scaffolds had only macroscale pores. FF scaffolds frozen at −20 °C and −80 °C had similar pore sizes, due to freezing in the support bath. The −20 °C and −80 °C FF scaffolds had porous struts with 63.55% ± 2.59% and 56.72% ± 13.17% strut porosity, respectively, while control scaffolds had a strut porosity of 3.15% ± 2.20%. The −20 °C and −80 °C FF scaffolds were softer than control scaffolds: they had pore wall stiffness of 0.17 ± 0.06 MPa and 0.23 ± 0.05 MPa, respectively, compared to 1.31 ± 0.39 MPa for the control. The FF scaffolds had increased resilience in bending compared with control. FF scaffolds supported MDA-MB-231 cell growth and had significantly greater cell numbers than control scaffolds. Cells formed clusters on the porous struts of FF scaffolds and had similar morphologies as the freeze cast scaffolds. The FF technique successfully introduced microscale porosity into the 3DP scaffold struts to produce hierarchical pore structures that enhanced MDA-MB-231 growth.
Numerical Studies on Damage Behavior of Recycled Aggregate Concrete Based on a 3D Model
Authors: Wang Yao , Zhao Huawei , Xu Minyao , Wu Chunyang , Fu Jiajia , Gao Lili , Kamel Mahmoud M. A.
Published: January 2020

This paper develops a 3D base force element method (BFEM) based on the potential energy principle. According to the BFEM, the stiffness matrix and node displacement of any eight-node hexahedral element are derived as a uniform expression. Moreover, this expression is explicitly expressed without a Gaussian integral. A 3D random numerical model of recycled aggregate concrete (RAC) is established. The randomness of aggregate was obtained by using the Monte Carlo random method. The effects of the recycled aggregate substitution and adhered mortar percentage on the elastic modulus and compressive strength are explored under uniaxial compression loading. In addition, the failure pattern is also studied. The obtained data show that the 3D BFEM is an efficient method to explore the failure mechanism of heterogeneous materials. The 3D random RAC model is feasible for characterizing the mesostructure of RAC. Both the substitution of recycled aggregate and the percentage of adhering mortar have a non-negligible influence on the mechanical properties of RAC. As the weak points in the specimen, the old interfacial transition zone (ITZ) and adhered mortar are the major factors that lead to the weakened properties of RAC. The first crack always appears in these weak zones, and then, due to the increase and transfer of stress, approximately two-to-three continuous cracks are formed in the 45°direction of the specimen.
Influence of Micro-Arc Oxidation Coatings on Stress Corrosion of AlMg6 Alloy
Authors: Kyzioł Lesław , Komarov Aleksandr
Published: January 2020

This paper shows results of a study on the corrosion behavior of micro-arc oxidation (MAO) coatings sampled from the AlMg6 alloy. The alloy was simultaneously subjected to a corrosive environment and static tensile stress. For comparative purposes, the tests were run for both coated samples and samples without coatings. The research was conducted at a properly prepared stand; the samples were placed in a glass container filled with 3.5% NaCl aqueous solution and stretched. Two levels of tensile stress were accepted for the samples: σ1 = 0.8R0.2 σ2 = R0.2, and the tests were run for two time intervals: t1 = 480 h and t2 = 1000 h. Prolonged stress corrosion tests (lasting up to 1000 h) showed that the samples covered with ceramic coatings demonstrated significantly higher corrosion resistance than the samples without the coatings. Protective properties of the coating could be explained by its structure. Surface pores were insignificant, and their depth was very limited. The porosity level of the main coating layer was 1%. Such a structure of coating and its phase composition provided high protective properties.
Evaluation of the Mechanical, Physical, and Anti-Fungal Properties of Flax Laboratory Papersheets with the Nanoparticles Treatment
Authors: Abo Elgat Wael A. A. , Taha Ayman S. , Böhm Martin , Vejmelková Eva , Mohamed Wael S. , Fares Yahia G. D. , Salem Mohamed Z. M.
Published: January 2020

In the present study, novel mixed additives of Chitosan or Paraloid B-72 combined with nanoparticles (NPs) of Ag, ZnO, or cellulose (NCL) were examined for their effects on the mechanical, optical, and fungal inhibition properties of the papersheets produced. The highest tensile, tear, and burst indices of the papersheets were observed for flax pulp treated with additives of Paraloid B-72 + ZnO NP (1%), Chitosan + ZnO NP (3%), and Chitosan + NCL (3%) at levels of 59.93 N·m/g, 18.45 mN·m2/g, and 6.47 kPa·m2/g, respectively. Chitosan + ZnO NP (1%) added to flax pulp showed the highest fungal mycelial inhibition (FMI) (1.85%) against Aspergillus flavus. Chitosan + Ag NP (1%) exhibited the highest FMI percentage (11.48%) when added to pulp against A. terreus. Pulp treated with Paraloid B-72 + Ag NP (1%) exhibited the highest activity against Stemphylium solani with an FMI value of 3.7%. The results indicate that the technological properties of the papersheets were enhanced with the addition of novel mixtures to the pulp.
Quantitatively Inferring Three Mechanisms from the Spatiotemporal Patterns
Authors: Zhang Kang , Hu Wen-Si , Liu Quan-Xing
Published: January 2020

Although the diversity of spatial patterns has gained extensive attention on ecosystems, it is still a challenge to discern the underlying ecological processes and mechanisms. Dynamical system models, such partial differential equations (PDEs), are some of the most widely used frameworks to unravel the spatial pattern formation, and to explore the potential ecological processes and mechanisms. Here, comparing the similarity of patterned dynamics among Allen–Cahn (AC) model, Cahn–Hilliard (CH) model, and Cahn–Hilliard with population demographics (CHPD) model, we show that integrated spatiotemporal behaviors of the structure factors, the density-fluctuation scaling, the Lifshitz–Slyozov (LS) scaling, and the saturation status are useful indicators to infer the underlying ecological processes, even though they display the indistinguishable spatial patterns. First, there is a remarkable peak of structure factors of the CH model and CHPD model, but absent in AC model. Second, both CH and CHPD models reveal a hyperuniform behavior with scaling of −2.90 and −2.60, respectively, but AC model displays a random distribution with scaling of −1.91. Third, both AC and CH display uniform LS behaviors with slightly different scaling of 0.37 and 0.32, respectively, but CHPD model has scaling of 0.19 at short-time scales and saturation at long-time scales. In sum, we provide insights into the dynamical indicators/behaviors of spatial patterns, obtained from pure spatial data and spatiotemporal related data, and a potential application to infer ecological processes.
Illnesses Encountered during Medical Volunteering in Takeo Province, Cambodia
Authors: Lee Hye-Yoon , Choi Sung Hun , Rim Jae Suk , Lim Ho-Kyung , Heo Young Soo , Shin Jeonghee , Aieng Rinet , Rim Chai Hong
Published: January 2020

Background and Objectives: Medical volunteering seeks to meet the clinical needs of underserved areas, but has been criticized for difficulties in addressing local health issues and resultant lack of sustainability. Our team has visited rural Cambodia annually since 2012. This study reports the illnesses encountered during the recent mission and share our experiences to improve the efficiency of medical volunteering. Materials and Methods: Infrastructure, such as public electricity or water, was unavailable, hence most medical care and records were hand-performed. We categorized (1) primary diagnoses (chief complaints) by duration of symptoms, and (2) primary and secondary diagnoses (illnesses that were not related to the chief complaint) by severity of illness since patients commonly reported multiple symptoms. Blood pressure and anthropometric values were also checked and analyzed. Results: We encountered 317 adult and 141 pediatric patients. Among adults, 61.3% had persistent chronic (>6 month) symptoms of their chief complaints. The commonest diagnoses of chronic symptoms were musculoarthritis (31.5%) and gastroesophageal reflux disease and/or gastritis (21.7%). Hypertension and/or cardiac problems were relatively common among males (13.6%). The most common diagnosis among the severest cases (specialized or intensive care recommended) was cardiac problems (14.8%), often with abnormalities in sonography or electrocardiogram. For children, the overwhelming majority of diagnoses were related to acute symptoms and low severity, and approximately half were cases of the common cold. Commonly prescribed drugs were antacids or mucosal protectors (31.3%), Non-steroidal anti-inflammatory drugs (NSAIDs) or other painkillers (27.6%), and antiparasites (17.7%) in adults, and NSAIDs (44.7%) and antiparasites (23.2%) in children. Among adults, 32.7% were diagnosed with hypertension, and body mass index (p = 0.003) and age (p < 0.001) were both correlated with hypertension and its grade. Conclusions: Our study offers practical help to volunteer health workers planning to visit Southeast Asia.
Systematic Study of the Impact of Pulsed Electric Field Parameters (Pulse/Pause Duration and Frequency) on ED Performances during Acid Whey Treatment
Authors: Dufton Guillaume , Mikhaylin Sergey , Gaaloul Sami , Bazinet Laurent
Published: January 2020

Processing acid whey is still a challenge for the dairy industry due to its high lactic acid and mineral contents. Their removal processes represent a high investment and running cost in addition to significant production of polluting effluents. A previous study showed that the use of electrodialysis with the application of pulsed electric fields (PEFs) was sufficiently efficient to produce dryable acid whey with reduced scaling issues during the process. In the present work, eight PEF conditions using different pulse/pause durations and frequencies were tested for 1) process optimization and 2) understanding of the underlying mechanisms involved in PEF process improvements. Best results were obtained for PEF conditions (5 s/5 s) and (15 s/15 s) with almost complete scaling mitigation and minimal energy consumption (5.3 ± 0.4 Wh/g of lactic acid vs. 9.33 ± 1.38 Wh/g for continuous current). Longer pause times also led to better divalent ion demineralization at the expense of sodium elimination induced by stronger affinity with the membrane and longer retention times. For the first time, PEF parameters of relatively low frequencies (<1) were studied in sub-limiting current conditions on a complex solution such as acid whey.
Methacrylate Coatings for Titanium Surfaces to Optimize Biocompatibility
Authors: Sun Argus , Ashammakhi Nureddin , Dokmeci Mehmet R.
Published: January 2020

Currently, there are more than 1.5 million knee and hip replacement procedures carried out in the United States. Implants have a 10–15-year lifespan with up to 30% of revision surgeries showing complications with osteomyelitis. Titanium and titanium alloys are the favored implant materials because they are lightweight and have high mechanical strength. However, this increased strength can be associated with decreased bone density around the implant, leading to implant loosening and failure. To avoid this, current strategies include plasma-spraying titanium surfaces and foaming titanium. Both techniques give the titanium a rough and irregular finish that improves biocompatibility. Recently, researchers have also sought to surface-conjugate proteins to titanium to induce osteointegration. Cell adhesion-promoting proteins can be conjugated to methacrylate groups and crosslinked using a variety of methods. Methacrylated proteins can be conjugated to titanium surfaces through atom transfer radical polymerization (ATRP). However, surface conjugation of proteins increases biocompatibility non-specifically to bone cells, adding to the risk of biofouling which may result in osteomyelitis that causes implant failure. In this work, we analyze the factors contributing to biofouling when coating titanium to improve biocompatibility, and design an experimental scheme to evaluate optimal coating parameters.
Genome Improvement and Core Gene Set Refinement of Fugacium kawagutii
Authors: Li Tangcheng , Yu Liying , Song Bo , Song Yue , Li Ling , Lin Xin , Lin Senjie
Published: January 2020

Cataloging an accurate functional gene set for the Symbiodiniaceae species is crucial for addressing biological questions of dinoflagellate symbiosis with corals and other invertebrates. To improve the gene models of Fugacium kawagutii, we conducted high-throughput chromosome conformation capture (Hi-C) for the genome and Illumina combined with PacBio sequencing for the transcriptome to achieve a new genome assembly and gene prediction. A 0.937-Gbp assembly of F. kawagutii were obtained, with a N50 > 13 Mbp and the longest scaffold of 121 Mbp capped with telomere motif at both ends. Gene annotation produced 45,192 protein-coding genes, among which, 11,984 are new compared to previous versions of the genome. The newly identified genes are mainly enriched in 38 KEGG pathways including N-Glycan biosynthesis, mRNA surveillance pathway, cell cycle, autophagy, mitophagy, and fatty acid synthesis, which are important for symbiosis, nutrition, and reproduction. The newly identified genes also included those encoding O-methyltransferase (O-MT), 3-dehydroquinate synthase, homologous-pairing protein 2-like (HOP2) and meiosis protein 2 (MEI2), which function in mycosporine-like amino acids (MAAs) biosynthesis and sexual reproduction, respectively. The improved version of the gene set (Fugka_Geneset _V3) raised transcriptomic read mapping rate from 33% to 54% and BUSCO match from 29% to 55%. Further differential gene expression analysis yielded a set of stably expressed genes under variable trace metal conditions, of which 115 with annotated functions have recently been found to be stably expressed under three other conditions, thus further developing the “core gene set” of F. kawagutii. This improved genome will prove useful for future Symbiodiniaceae transcriptomic, gene structure, and gene expression studies, and the refined “core gene set” will be a valuable resource from which to develop reference genes for gene expression studies.
Round Robin into Best Practices for the Determination of Indentation Size Effects
Authors: Ruiz-Moreno Ana , Hähner Peter , Kurpaska Lukasz , Jagielski Jacek , Spätig Philippe , Trebala Michal , Hannula Simo-Pekka , Merino Susana , de Diego Gonzalo , Namburi Hygreeva , Libera Ondrej , Terentyev Dimitry , Khvan Tymofii , Heintze Cornelia , Jennett Nigel
Published: January 2020

The paper presents a statistical study of nanoindentation results obtained in seven European laboratories that have joined a round robin exercise to assess methods for the evaluation of indentation size effects. The study focuses on the characterization of ferritic/martensitic steels T91 and Eurofer97, envisaged as structural materials for nuclear fission and fusion applications, respectively. Depth-controlled single cycle measurements at various final indentation depths, force-controlled single cycle and force-controlled progressive multi-cycle measurements using Berkovich indenters at room temperature have been combined to calculate the indentation hardness and the elastic modulus as a function of depth applying the Oliver and Pharr method. Intra- and inter-laboratory variabilities have been evaluated. Elastic modulus corrections have been applied to the hardness data to compensate for materials related systematic errors, like pile-up behaviour, which is not accounted for by the Oliver and Pharr theory, and other sources of instrumental or methodological bias. The correction modifies the statistical hardness profiles and allows determining more reliable indentation size effects.
Addendum: Zurob, E., et al. Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces. Nanomaterials 2019, 9, 49
Authors: Zurob Elsie , Dennett Geraldine , Gentil Dana , Montero-Silva Francisco , Gerber Ulrike , Naulín Pamela , Gómez Andrea , Fuentes Raúl , Lascano Sheila , Rodrigues da Cunha Thiago Henrique Rodrigues da , Ramírez Cristian , Henríquez Ricardo , Campo Valeria Del , Barrera Nelson , Wilkens Marcela , Parra Carolina
Published: January 2020

The authors wish to make the following corrections to this paper [...]
Energy Transfer in Vials Nested in a Rack System During Lyophilization
Authors: Daller Sarah , Friess Wolfgang , Schroeder Rudolf
Published: January 2020

Small batch sizes are a consequence of more personalized medicine and reflect a trend in the biopharmaceutical industry. Freeze drying of vials nested in a rack system is a tool used in new flexible pilot scale processing lines. Understanding of heat transfer mechanisms in the rack loaded with vials not in direct contact with each other is necessary to ensure high quality. Lyophilization in the rack vial system enables a homogeneous drying with a reduced edge-vial-effect and shielding against radiation from surrounding components, e.g., the chamber wall. Due to the separation effect of the rack, direct shelf contact contributes approx. 40% to the overall energy transfer to the product during primary drying. Hence overall the rack is a flexible, robust tool for small batch production, which ensures a controlled heat transfer resulting in a uniform product.
Oxygen and ROS in Photosynthesis
Authors: Khorobrykh Sergey , Havurinne Vesa , Mattila Heta , Tyystjärvi Esa
Published: January 2020

Oxygen is a natural acceptor of electrons in the respiratory pathway of aerobic organisms and in many other biochemical reactions. Aerobic metabolism is always associated with the formation of reactive oxygen species (ROS). ROS may damage biomolecules but are also involved in regulatory functions of photosynthetic organisms. This review presents the main properties of ROS, the formation of ROS in the photosynthetic electron transport chain and in the stroma of chloroplasts, and ROS scavenging systems of thylakoid membrane and stroma. Effects of ROS on the photosynthetic apparatus and their roles in redox signaling are discussed.
Hierarchical Cs–Pollucite Nanozeolite Modified with Novel Organosilane as an Excellent Solid Base Catalyst for Claisen–Schmidt Condensation of Benzaldehyde and Acetophenone
Authors: Mohammad S. Aleid Ghadah , Khoerunnisa Fitri , Rigolet Severinne , Daou T. Jean , Ling Tau-Chuan , Ng Eng-Poh
Published: January 2020

Cs–pollucite can be a potential solid base catalyst due to the presence of (Si-O-Al)−Cs+ basic sites. However, it severely suffers from molecular diffusion and pore accessibility problems due to its small micropore opening. Herein, we report the use of new organosilane, viz. dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (TPOAC), as a promising pore-expanding agent to develop the hierarchical structure in nanosized Cs–pollucite. In respect to this, four different amounts of TPOAC were added during the synthesis of hierarchical Cs–pollucite (CP-x, x = 0, 0.3, 1.0, or 2.0, where x is the molar ratio of TPOAC) in order to investigate the effects of TPOAC in the crystallization process of Cs–pollucite. The results show that an addition of TPOAC altered the physico-chemical and morphological properties of hierarchical Cs–pollucite, such as the crystallinity, crystallite size, pore size distribution, surface areas, pore volume, and surface basicity. The prepared solids were also tested in Claisen–Schmidt condensation of benzaldehyde and acetophenone, where 82.2% of the conversion and 100% selectivity to chalcone were achieved by the CP-2.0 catalyst using non-microwave instant heating (200 °C, 100 min). The hierarchical CP-2.0 nanocatalyst also showed better catalytic performance than other homogenous and heterogeneous catalysts and displayed a high catalyst reusability with no significant deterioration in the catalytic performance even after five consecutive reaction runs.
Drying Kinetics, Grinding Characteristics, and Physicochemical Properties of Broccoli Sprouts
Authors: Dziki Dariusz , Habza-Kowalska Ewa , Gawlik-Dziki Urszula , Miś Antoni , Różyło Renata , Krzysiak Zbigniew , Hassoon Waleed H.
Published: January 2020

In this study, we studied the drying process, grinding characteristics and physicochemical characteristics of broccoli sprouts (BS). The seeds of broccoli were germinated at 20 °C for 3 and 6 days. Then, the seeds were air- and freeze-dried, and the Page model was used for prediction of drying kinetics of broccoli sprouts. It was observed that the drying time of BS decreased about twofold as the air-drying temperature increased from 40 to 80 °C. An increasing the air-drying temperature from 40 to 80 °C decreased the drying time by approximately twofold. Freeze-drying of sprouts took the longest drying time. Germination of seeds significantly decreased the value of grinding energy requirements, and the ground sprouts exhibited a different grinding pattern in comparison to ground non-germinated seeds. In terms of color parameters, the highest lightness and yellowness were found for freeze-dried sprouts. Redness and yellowness of sprouts increased with an increase in the air-drying temperature. The lowest total color difference was obtained for the freeze-dried sprouts. Higher drying temperature resulted in lower total phenolics content (TPC) and decreased antioxidant activity (AA). The highest TPC and AA were observed in air-dried sprouts (40 °C) and freeze-dried sprouts after 6 days of germination.
Devotion, Paintings, and the House: The Collections of Ercole and Giuseppe Branciforti, Princes of Scordia (Palermo, 1687–1720)
Authors: Viola Valeria
Published: January 2020

This paper interrogates familial devotion and its relationship with parts of the house other than the chapel. In detail, it aims to problematize the issue of the devotional/non-devotional use of paintings inside the house by moving the focus from this dual opposition to the active role of canvases, broadly defined. Informed by Jacques Derrida’s and Pierre Bourdieu’s writings, this paper argues for the structural nature of the paintings inside the house and their meaningful correlation with both the arrangement of the domestic interior and the practices of people experiencing those spaces. To do this, the paper challenges the overwhelming attention paid by early-modern scholars to Northern and central Italy and investigates a precise case study, i.e., Palazzo Scordia in Palermo (Sicily). The research draws upon primary sources and amongst these, upon two detailed inventories of furniture referring to two subsequent generations of an aristocratic clan residing in Palermo between the seventeenth and the eighteenth century, i.e., Ercole and Giuseppe Branciforti, princes of Scordia.