bluuebunny/crossref_metadata_2025 huggingface dataset:
Ship Classification Based on Attention Mechanism and Multi-Scale Convolutional Neural Network for Visible and Infrared Images
Authors: Ren Yongmei , Yang Jie , Guo Zhiqiang , Zhang Qingnian , Cao Hui
Published: November 2020

Visible image quality is very susceptible to changes in illumination, and there are limitations in ship classification using images acquired by a single sensor. This study proposes a ship classification method based on an attention mechanism and multi-scale convolutional neural network (MSCNN) for visible and infrared images. First, the features of visible and infrared images are extracted by a two-stream symmetric multi-scale convolutional neural network module, and then concatenated to make full use of the complementary features present in multi-modal images. After that, the attention mechanism is applied to the concatenated fusion features to emphasize local details areas in the feature map, aiming to further improve feature representation capability of the model. Lastly, attention weights and the original concatenated fusion features are added element by element and fed into fully connected layers and Softmax output layer for final classification output. Effectiveness of the proposed method is verified on a visible and infrared spectra (VAIS) dataset, which shows 93.81% accuracy in classification results. Compared with other state-of-the-art methods, the proposed method could extract features more effectively and has better overall classification performance.
A Cloudware Architecture for Collaboration of Multiple AGVs in Indoor Logistics: Case Study in Fabric Manufacturing Enterprises
Authors: Okumuş Fatih , Dönmez Emrah , Kocamaz Adnan Fatih
Published: November 2020

In Industry 4.0 compatible workshops, the demand for Automated Guided Vehicles (AGVs) used in indoor logistics systems has increased remarkably. In these indoor logistics systems, it may be necessary to execute multiple transport tasks simultaneously using multiple AGVs. However, some challenges require special solutions for AGVs to be used in industrial autonomous transportation. These challenges can be addressed under four main headings: positioning, optimum path planning, collision avoidance and optimum task allocation. The solutions produced for these challenges may require special studies that vary depending on the type of tasks and the working environment in which AGVs are used. This study focuses on the problem of automated indoor logistics carried out in the simultaneous production of textile finishing enterprises. In the study, a centralized cloud system that enables multiple AGVs to work in collaboration has been developed. The finishing enterprise of a denim manufacturing factory was handled as a case study and modelling of mapping-planning processes was carried out using the developed cloud system. In the cloud system, RestFul APIs, for mapping the environment, and WebSocket methods, to track the locations of AGVs, have been developed. A collaboration module in harmony with the working model has been developed for AGVs to be used for fabric transportation. The collaboration module consists of task definition, battery management-optimization, selection of the most suitable batch trolleys (provides mobility of fabrics for the finishing mills), optimum task distribution and collision avoidance stages. In the collaboration module, all the finishing processes until the product arrives the delivery point are defined as tasks. A task allocation algorithm has been developed for the optimum performance of these tasks. The multi-fitness function that optimizes the total path of the AGVs, the elapsed time and the energy spent while performing the tasks have been determined. An assignment matrix based on K nearest neighbor (k-NN) and permutation possibilities was created for the optimal task allocation, and the most appropriate row was selected according to the optimal path totals of each row in the matrix. The D* Lite algorithm has been used to calculate the optimum path between AGVs and goals by avoiding static obstacles. By developing simulation software, the problem model was adapted and the operation of the cloud system was tested. Simulation results showed that the developed cloud system was successfully implemented. Although the developed cloud system has been applied as a case study in fabric finishing workshops with a complex structure, it can be used in different sectors as its logistic processes are similar.
Linking Soil Acidity to P Fractions and Exchangeable Base Cations under Increased N and P Fertilization of Mono and Mixed Plantations in Northeast China
Authors: Zarif Nowsherwan , Khan Attaullah , Wang Qingcheng
Published: November 2020

Atmospheric N deposition is increasing worldwide, especially in China, significantly affecting soil health, i.e., increasing soil acidification. The northern region of China is considered to be one of the N deposition points in Asia, ranging from 28.5 to 100.4 N ha−1yr−1. Phosphorus (P) is the limiting factor in the temperate ecosystem and an important factor that makes the ecosystem more susceptible to N-derived acidification. However, it remained poorly understood how the soil acidification process affects soil P availability and base cations in the temperate region to increased N deposition. To address this question, in May 2019, a factorial experiment was conducted under N and P additions with different plantations in Maoershan Experimental Forest Farm, Northeast China, considering species and fertilization as variables. The effective acidity (EA) increased by N and NP fertilizations but was not significantly affected by P fertilization. Similarly, the pH, base saturation percentage (BS%), calcium (Ca2+), and magnesium (Mg2+) were decreased under N addition, while the Al:Ca ratio increased, whereas NaHCO3 inorganic phosphorus (Pi) and NaOH organic phosphorus (Po) significantly decreased under N enrichments. However, NaOH Pi increased in N-enriched plots, while H2O Pi and NaHCO3 Pi increased under the P addition. Thus, the results suggest that the availability of N triggers the P dynamics by increasing the P uptake by trees. The decrease in base cations, Ca2+, and Mg2+ and increase in exchangeable Fe3+ and Al3+ ions are mainly responsible for soil acidification and lead to the depletion of soil nutrients, which, ultimately, affects the vitality and health of forests, while the P addition showed a buffering effect but could not help to mitigate the soil acidity.
Lightning Solvers for Potential Flows
Authors: Baddoo Peter J.
Published: November 2020

We present a method for computing potential flows in planar domains. Our approach is based on a new class of techniques, known as “lightning solvers”, which exploit rational function approximation theory in order to achieve excellent convergence rates. The method is particularly suitable for flows in domains with corners where traditional numerical methods fail. We outline the mathematical basis for the method and establish the connection with potential flow theory. In particular, we apply the new solver to a range of classical problems including steady potential flows, vortex dynamics, and free-streamline flows. The solution method is extremely rapid and usually takes just a fraction of a second to converge to a high degree of accuracy. Numerical evaluations of the solutions are performed in a matter of microseconds and can be compressed further with novel algorithms.
Regionalized Strategies for Food Loss and Waste Management in Spain under a Life Cycle Thinking Approach
Authors: Hoehn Daniel , Laso Jara , Cristóbal Jorge , Ruiz-Salmón Israel , Butnar Isabela , Borrion Aiduan , Bala Alba , Fullana-i-Palmer Pere , Vázquez-Rowe Ian , Aldaco Rubén , Margallo María
Published: November 2020

Food loss and waste (FLW) has become a central concern in the social and political debate. Simultaneously, using FLW as a bioenergy source could significantly contribute to closing the carbon cycle by reintroducing energy into the food supply chain. This study aims to identify best strategies for FLW management in each of the 17 regions in Spain, through the application of a Life Cycle Assessment. To this end, an evaluation of the environmental performance over time between 2015 and 2040 of five different FLW management scenarios implemented in a framework of (i) compliance and (ii) non-compliance with the targets of the Paris Agreement was performed. Results revealed savings in the consumption of abiotic resources in those regions in which thermal treatment has a strong presence, although their greenhouse gas (GHG) emissions in a scenario of compliance with climate change targets are higher. In contrast, scenarios that include anaerobic digestion and, to a lesser extent those applying aerobic composting, present lower impacts, including climate change, suggesting improvements of 20–60% in non-compliance and 20–80% in compliance with Paris Agreement targets, compared to the current scenarios.
A Cross-Cultural Evaluation of Liking and Perception of Salted Butter Produced from Different Feed Systems
Authors: C. Garvey Emer , Sander Thorsten , O’Callaghan Tom F. , Drake MaryAnne , Fox Shelley , G. O’Sullivan Maurice , Kerry Joseph P. , Kilcawley Kieran N.
Published: November 2020

Perception and liking among Irish, German and USA consumers of salted butter produced from different feed systems—outdoor grass (FS-GRSS), grass/clover (FS-CLVR), and indoor concentrate (FS-TMR)—was investigated. A consumer study was conducted in all three countries. Irish and German assessors participated in ranking descriptive analysis (RDA), whereas descriptive analysis (DA) was carried out by a trained panel in the USA. Volatile analysis was conducted to identify differences in aroma compounds related to cow diet. Overall, there was no significant difference in overall liking of the butters, among USA, German and Irish consumers, although cross-cultural preferences were evident. Sensory attribute differences based on cow diet were evident across the three countries, as identified by German and Irish assessors and trained USA panelists, which are likely influenced by familiarity. The abundance of specific volatile aromatic compounds, especially some aldehydes and ketones, were significantly impacted by the feed system and may also contribute to some of the perceived sensory attribute differences in these butters.
A Revisit to the Formation and Mitigation of 3-Chloropropane-1,2-Diol in Palm Oil Production
Authors: Arris Farrah Aida , Thai Vincent Tiang Soon , Manan Wan Nabilah , Sajab Mohd Shaiful
Published: November 2020

Process-based contaminants in food—particularly in vegetable oils—have been a topic of interest due to their potential health risk on humans. Oral consumption above the tolerable daily intake might result in health risks. Therefore, it is critical to correctly address the food contaminant issues with a proper mitigation plan, in order to reduce and subsequently remove the occurrence of the contaminant. 3-monochloropropane-1,3-diol (3-MCPD), an organic chemical compound, is one of the heat- and process-induced food contaminants, belonging to a group called chloropropanols. This review paper discusses the occurrence of the 3-MCPD food contaminant in different types of vegetable oils, possible 3-MCPD formation routes, and also methods of reduction or removal of 3-MCPD in its free and bound esterified forms in vegetable oils, mostly in palm oil due to its highest 3-MCPD content.
Tiger Nut (Cyperus esculentus) as a Functional Ingredient in Gluten-Free Extruded Snacks
Authors: Gasparre Nicola , Pan James , da Silva Alves Priscila Leal , Rosell Cristina M. , De J. Berrios Jose
Published: November 2020

Tiger nut (TN) is a nutritious source of gluten-free flour, used generally in healthy beverages, but its incorporation in gluten-free extruded snacks has not been explored. TN flour was blended at different concentrations (up to 70%) with rice flour and soluble fiber, for the development of gluten-free snacks on a twin-screw extruder. The effect of TN inclusion in the formulations was evaluated on relevant physiochemical characteristics of the snacks. Viscoamylograph of the raw formulations showed that TN addition increased (p < 0.01) onset temperature and delayed peak viscosity. In the extruded flours, TN contributed to limit the starch degradation during extrusion. Diameter, expansion ratio, true density, and total pore volume of the extrudates were reduced (pf < 0.01) by the increased TN content in the formulations, while bulk density rose. The surfaces of the extruded snacks were modified by the increasing inclusion of TN in substitution of rice in the formulations. Extrudates containing 10% TN showed the best overall texture profile. Moreover, TN addition enhanced the ash and protein content of the snacks and increased their total antioxidant activity. This study demonstrated that incorporation of 10% TN flour into rice-based formulation was suitable for making gluten-free snacks with acceptable physical properties.
Characterization of Buckwheat Beverages Fermented with Lactic Acid Bacterial Cultures and Bifidobacteria
Authors: Kowalska Ewa , Ziarno Małgorzata
Published: November 2020

This study aimed to examine the effect of four different industrial starter cultures containing lactic acid bacteria (LAB) and bifidobacteria on selected characteristics of beverages prepared from buckwheat and stored at 4 °C for 28 days. This study included the determination of pH during fermentation and during refrigerated storage, determination of the number of LAB and bifidobacteria, and chromatographic analysis of carbohydrates. This study showed that the tested starter cultures effectively fermented the buckwheat beverage. There was a sufficient number of viable cells in the starter microflora for the obtained beverages to exhibit potential health-promoting properties. Beverages had stable pH values during refrigerated storage. The stored beverages showed changes in the content of selected carbohydrates, which indicates the constant biochemical activity of the present starter microflora. This study provides useful references on the metabolism of LAB in plant-based beverages.
Genome-Wide Identification and Molecular Characterization of the Growth-Regulating Factors-Interacting Factor Gene Family in Tomato
Authors: Ai Guo , Zhang Dedi , Huang Rong , Zhang Shiqi , Li Wangfang , Ahiakpa John K. , Zhang Junhong
Published: November 2020

Growth-regulating factors-interacting factor (GIF) proteins play crucial roles in the regulation of plant growth and development. However, the molecular mechanism of GIF proteins in tomato is poorly understood. Here, four SlGIF genes (named SlGRF1a, SlGIF1b, SlGIF2, and SlGIF3) were identified from the tomato genome and clustered into two major clades by phylogenetic analysis. The gene structure and motif pattern analyses showed similar exon/intron patterns and motif organizations in all the SlGIFs. We identified 33 cis-acting regulatory elements (CAREs) in the promoter regions of the SlGIFs. The expression profiling revealed the four GIFs are expressed in various tissues and stages of fruit development and induced by phytohormones (IAA and GA). The subcellular localization assays showed all four GIFs were located in nucleus. The yeast two-hybrid assay indicated various growth-regulating factors (SlGRFs) proteins interacted with the four SlGIF proteins. However, SlGRF4 was a common interactor with the SlGIF proteins. Moreover, a higher co-expression relationship was shown between three SlGIF genes and five SlGRF genes. The protein association network analysis found a chromodomain helicase DNA-binding protein (CHD) and an actin-like protein to be associated with the four SlGIF proteins. Overall, these results will improve our understanding of the potential functions of GIF genes and act as a base for further functional studies on GIFs in tomato growth and development.
The Scientific Landscape of November 23rd, 1980 Irpinia-Basilicata Earthquake: Taking Stock of (Almost) 40 Years of Studies
Authors: Gizzi Fabrizio Terenzio , Potenza Maria Rosaria
Published: November 2020

The November 23rd, 1980 Irpinia-Basilicata (Southern Italy) earthquake is one of the strongest earthquakes ever occurred in Italy. The earthquake was a natural laboratory for the scientific community, which was engaged highly and promptly in investigating the event, thus publishing a flood of papers in different research areas over time. Just these research outputs are the focus of the article, which examines, with a tailored methodological approach, the international and national (Italian) studies started and advanced since the occurrence of the earthquake. First, we built and analyzed statistically two bibliographic databanks regarding the earthquake studies: (a) the international version of IRpinia Bibliographic databASE (IR_BASE_ENG), selecting and standardizing the pertinent scientific documents extracted from Scopus, Web of Science, and other databases and (b) the national version of the database (IR_BASE_IT) using the Google Scholar search engine to search for the most relevant papers in Italian. Second, IR_BASE_ENG was analyzed in a bibliometric perspective through the data mining VOSviewer software (Waltman et al., 2010) that builds co-occurrence term maps useful in perspective of investigating the wide-ranging studies on the earthquake. Third, taking a cue from this network analysis, we recognized the main research topics and performed a minireview of the related international studies, integrating in it a quick reference to the literature in Italian. Finally, we associated the scientific outputs to each cluster/topic, also performing the frequency analysis of the published documents for each subject, thus gaining information on the temporal trends of studies and getting a more exhaustive evidence of the scientific landscape on the earthquake over the last 40 years.
Rapid Telehealth Implementation during the COVID-19 Global Pandemic: A Rapid Review
Authors: Lieneck Cristian , Garvey Joseph , Collins Courtney , Graham Danielle , Loving Corein , Pearson Raven
Published: November 2020

The implementation and continued expansion of telehealth services assists a variety of health care organizations in the delivery of care during the current COVID-19 global pandemic. However, limited research has been conducted on recent, rapid telehealth implementation and expansion initiatives regarding facilitators and barriers surrounding the provision of quality patient care. Our rapid review evaluated the literature specific to rapid telehealth implementation during the current COVID-19 pandemic from three research databases between January 2020 and May 2020 and reported using preferred reporting items for systematic reviews and meta-analyses (PRISMA). The results indicate the rapid implementation and enhanced use of telehealth during the COVID-19 pandemic in the United States surrounding the facilitators and barriers to the provision of patient care, which are categorized into three identified themes: (1) descriptive process-oriented implementations, (2) the interpretation and infusion of the CARES Act of 2020 telehealth exemptions related to the relaxation of patient privacy and security (HIPAA) protocols, and (3) the standard of care protocols and experiences addressing organizational liability and the standard of care. While the study limitation of sample size exists (n = 21), an identification of rapid telehealth implementation advancements and challenges during the current pandemic may assist health care organizations in the delivery of ongoing quality care during the COVID-19 pandemic.
Clinical Characteristics of the COVID-19 Patients with Pneumonia Detected by Computerized Tomography but Negative for Infiltration by X-ray
Authors: Acharya Dilaram , Park Jungi , Lee Yebong , Hamm In Suk , Lee Dong Seok , Moon Seong-Su , Lee Kwan
Published: November 2020

Coronavirus Disease 2019 (COVID-19) has rapidly spread to all corners of the globe. Different diagnostic tools, such as Chest X-ray (CXR), lung ultrasound (LUS), and computerized tomography (CT), have been used to detect active pneumonic lesions associated with COVID-19 with their varying degrees of sensitivity and specificity. This study was undertaken to investigate the clinical characteristics of COVID-19 patients with a pneumonic lung lesion detected by CT that is not detected by CXR. A total of 156 COVID-19 patients hospitalized at three nationally designated South Korean hospitals with no active lesion detected by CXR but on clinical suspicion of pneumonia underwent the CT examination and were enrolled. Medical records, which included demographic and clinical features, including comorbidity, symptoms, radiological, and laboratory findings on admission, were reviewed and analyzed. The risk factors of pneumonia detected by CT for patients without an active lesion detected by CXR were investigated. Of the 156 patients without an active lesion detected by CXR, 35 (22.44%) patients were found to have pneumonia by CT. The patients with pneumonia defined by CT were older than those without (64.1 years vs. 41.2 years). Comorbidities such as hypertension, diabetes, cardiovascular disease, preexisting stroke, and dementia were more common among patients with pneumonia defined by CT than those without. Serum albumin level, C-reactive protein (CRP), stroke, and age ≥ 70 years were significantly associated with pneumonia defined by CT after adjustment for age. In multivariable regression analysis, serum albumin level (adjusted odds ratio (AOR) = 0.123, 95% CI = (0.035–0.429)) and preexisting stroke (AOR = 11.447, 95% CI = (1.168–112.220)) significantly and independently predicted pneumonia detection by CT. Our results suggest that CT scans should be performed on COVID-19 patients negative for a pneumonic lung lesion by CXR who are suspected to be pneumonic on clinical grounds. In addition, older patients with a lower albumin level and a preexisting stroke should be checked for the presence of pneumonia despite a negative CXR finding for an active lesion.
Prevalence and Subtype Distribution of Blastocystis Infection in Patients with Diabetes Mellitus in Thailand
Authors: Popruk Noppon , Prasongwattana Satakamol , Mahittikorn Aongart , Palasuwan Attakorn , Popruk Supaluk , Palasuwan Duangdao
Published: November 2020

Diabetes mellitus (DM) is a major global public health problem with an increasing prevalence. DM increases the risk of infections caused by bacteria, fungi, viruses, and parasites. We examined the prevalence, subtypes, and risk factors of Blastocystis infection in patients with and without DM in central Thailand. Stool samples and questionnaires were obtained from 130 people in the DM group and 100 people in the non-DM group. Blastocystis infection was identified via a nested polymerase chain reaction and subtyped via sequencing of the partial small-subunit ribosomal RNA (SSU rRNA) gene. Analysis of potential risk factors was conducted via binary logistic regression. The overall prevalence of Blastocystis infection was 10.8%, including rates of 9% and 12.3% in the non-DM and DM groups, respectively. The most prevalent subtype was ST3, followed by ST1, and ST4. Factors that potentially increased the risk of Blastocystis infection include patients being >65 years old, the presence of DM, a DM duration of ≥10 years, a low level of education, and animal ownership. In conclusion, this is the first study of Blastocystis infection in DM, and a high prevalence was found among this population. Therefore, health education promoting sanitation and hygiene is necessary to reduce and prevent infection in the community.
Current Trends of Neutrophil Biology
Authors: Kobayashi Yoshiro
Published: November 2020

Neutrophils are short-lived and terminally differentiated cells, and therefore, have been considered as effector cells to phagocytose pathogens and kill them or damage tissues [...]
Parasitoids of Chrysopidae Eggs in Sinaloa Mexico
Authors: Ramírez-Ahuja María de Lourdes , Garza-González Enrique , Talamas Elijah J. , Gómez-Govea Mayra A. , Rodríguez-Pérez Mario A. , Zambrano-Robledo Patricia , Rebollar-Tellez Eduardo , Rodríguez-Sanchez Iram P.
Published: November 2020

The eggs parasitoids Myartsevaia chrysopae (Crawford) (Hymenoptera: Encyrtidae), Telenomus lobatus Johnson, Telenomus tridentatus Johnson (Hymenoptera: Scelionidae) and Trichogramma atopovirilia Oatman and Platner (Hymenoptera: Trichogrammatidae) are reported for the first time or in new localities in Mexico. Their occurrence was first discovered in 2018 during a survey of parasitism on chrysopid eggs, conducted on Sorghum bicolor L. Moench (Poales: Poaceae) and Zea mays L. (Poales: Poaceae) in different locations in Sinaloa, Mexico. The identity of the parasitoids was determined by morphology and for both species of Telenomus the barcode region of the cytochrome oxidase 1 gene (CO1) was generated to facilitate molecular diagnosis of these species in future studies.
Comparative Fecal Metabolomes of Silkworms Being Fed Mulberry Leaf and Artificial Diet
Authors: Qin DaoYuan , Wang GenHong , Dong ZhaoMing , Xia QingYou , Zhao Ping
Published: November 2020

Metabonomics accurately monitors the precise metabolic responses to various dietary patterns. Metabolic profiling allows simultaneous measurement of various fecal metabolites whose concentrations may be affected by food intake. In this study, we analyzed the fecal metabolomes of silkworm (Bombyx mori) larvae reared on fresh mulberry leaves and artificial diets. 57 differentially expressed metabolites were identified by gas chromatography–mass spectrometry. Of these, 39 were up-regulated and 18 were downregulated in the mulberry leaf meal group. Most of the amino acids, carbohydrates and lipids associated with physical development and silk protein biosynthesis were enriched in silkworms reared on mulberry leaves. In contrast, the urea, citric acid, D-pinitol, D-(+)-cellobiose and N-acetyl glucosamine levels were relatively higher in the silkworm feeding on the artificial diets. The findings of this study help clarify the association between diet and metabolic profiling.
Cytocompatibility and Bioactive Properties of Hydraulic Calcium Silicate-Based Cements (HCSCs) on Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs): A Systematic Review of In Vitro Studies
Authors: Sanz José Luis , Forner Leopoldo , Llena Carmen , Guerrero-Gironés Julia , Melo María , Rengo Sandro , Spagnuolo Gianrico , Rodríguez-Lozano Francisco Javier
Published: November 2020

The implementation of hydraulic calcium silicate-based endodontic cements (HCSCs) in biologically based endodontic procedures for the primary dentition has been recently investigated, focusing on the biological response of stem cells from human exfoliated deciduous teeth (SHEDs) towards them. The present systematic review aimed to present a qualitative synthesis of the available literature consisting of in vitro assays, which assessed the cytocompatibility and bioactive properties of HCSCs in direct contact with SHEDs. Following the PRISMA statement, an electronic database search was carried out in Medline, Scopus, Embase, Web of Science, and SciELO on March 31st and updated on November 16th, 2020. In vitro studies evaluating the biological response of SHEDs to the treatment with HCSCs were eligible. Within the term biological response, assays assessing the cytocompatibility (i.e., cell viability, migration, proliferation), cell plasticity or differentiation (i.e., osteo/odontogenic marker expression), and bioactivity or biomineralization (i.e., mineralized nodule formation) were included. A total of seven studies were included after the selection process. The study sample comprised an extensive range of cell viability, migration, proliferation, adhesion, and bioactivity assays regarding the biological response of SHEDs towards five different commercially available HCSCs (MTA, ProRoot MTA, Biodentine, iRoot BP Plus, and Theracal LC). Biodentine, MTA, and iRoot BP Plus showed significant positive results in cytocompatibility and bioactivity assays when cultured with SHEDs. The results from in vitro assays assessing the cytocompatibility and bioactivity of the HCSCs MTA, Biodentine, and iRoot BP Plus towards SHEDs support their use in vital pulp treatment for the primary dentition.
Diagnostic Accuracy and Clinical Impact of Sentinel Lymph Node Sampling in Endometrial Cancer at High Risk of Recurrence: A Meta-Analysis
Authors: Lecointre Lise , Lodi Massimo , Faller Émilie , Boisramé Thomas , Agnus Vincent , Baldauf Jean-Jacques , Gallix Benoît , Akladios Chérif
Published: November 2020

Purpose. To assess the value of sentinel lymph node (SLN) sampling in high risk endometrial cancer according to the ESMO-ESGO-ESTRO classification. Methods. We performed a comprehensive search on PubMed for clinical trials evaluating SLN sampling in patients with high risk endometrial cancer: stage I endometrioid, grade 3, with at least 50% myometrial invasion, regardless of lymphovascular space invasion status; or stage II; or node-negative stage III endometrioid, no residual disease; or non-endometrioid (serous or clear cell or undifferentiated carcinoma, or carcinosarcoma). All patients underwent SLN sampling followed by pelvic with or without para-aortic lymphadenectomy. Results. We included 17 original studies concerning 1322 women. Mean detection rates were 89% for unilateral and 68% for bilateral. Pooled sensitivity was 88.5% (95%CI: 81.2–93.2%), negative predictive value was 96.0% (95%CI: 93.1–97.7%), and false negative rate was 11.5% (95%CI: 6.8; 18.8%). We noted heterogeneity in SLN techniques between studies, concerning the tracer and its detection, the injection site, the number of injections, and the surgical approach. Finally, we found a correlation between the number of patients included and the SLN sampling performances. Discussion. This meta-analysis estimated the SLN sampling performances in high risk endometrial cancer patients. Data from the literature show the feasibility, the safety, the limits, and the impact on surgical de-escalation of this technique. In conclusion, our study supports the hypothesis that SLN sampling could be a valuable technique to diagnose lymph node involvement for patients with high risk endometrial cancer in replacement of conventional lymphadenectomy. Consequently, randomized clinical trials are necessary to confirm this hypothesis.
New Insights into the Microbial Profiles of Infected Root Canals in Traumatized Teeth
Authors: Manoharan Lokeshwaran , Brundin Malin , Rakhimova Olena , Chávez de Paz Luis , Romani Vestman Nelly
Published: November 2020

Traumatic dental injuries in young individuals are often exposed to the invasion of oral microorganisms that leads to pulp necrosis. Infective necrosis in permanent teeth not-fully-developed causes aberrant root formation. Regeneration endodontic treatments (RETs) have shown promising results by promoting continued root development by stem cells. Critical to the success of RET is the thorough disinfection of the pulpal space. To establish effective antimicrobial protocols for root canal disinfection, the invading microorganisms need to be identified. In the present study, we use a combination of culture-based and high-throughput molecular sequencing techniques to investigate the microbial profiles from traumatized teeth (30 cases) and controls, i.e., teeth with pulp infections not caused by trauma (32 cases). Overall, a high microbial diversity in traumatized necrotic teeth was observed. Eubacterium yurii subsps. yurii and margaretiae, as well as key ‘bridging oral species’ F. nucleatum sp., Polymorphum and Corynebacterium matruchotti, were highly associated with traumatized teeth. The microbial compositions of traumatized teeth differed considerably from those of infected teeth not caused by trauma. Age and tooth position also influence microbial compositions. In conclusion, we show that the root canal microflora of traumatized teeth is highly diverse, and it differs from root canal infections not caused by trauma.
Inhibition of 11β-HSD1 Expression by Insulin in Skin: Impact for Diabetic Wound Healing
Authors: Brazel Christina B. , Simon Jan C. , Tuckermann Jan P. , Saalbach Anja
Published: November 2020

Chronic, non-healing wounds impose a great burden on patients, professionals and health care systems worldwide. Diabetes mellitus (DM) and obesity are globally highly prevalent metabolic disorders and increase the risk for developing chronic wounds. Glucocorticoids (GCs) are endogenous stress hormones that exert profound effects on inflammation and repair systems. 11-beta-hydroxysteroid dehydrogenase 1 (11β-HSD1) is the key enzyme which controls local GC availability in target tissues such as skin. Since treatment with GCs has detrimental side effects on skin integrity, causing atrophy and delayed wound healing, we asked whether the dysregulated expression of 11β-HSD1 and consequently local GC levels in skin contribute to delayed wound healing in obese, diabetic db/db mice. We found increased expression of 11β-HSD1 during disturbed wound healing and in the healthy skin of obese, diabetic db/db mice. Cell analysis revealed increased expression of 11β-HSD1 in fibroblasts, myeloid cells and dermal white adipose tissue from db/db mice, while expression in keratinocytes was unaffected. Among diabetes- and obesity-related factors, insulin and insulin-like growth factor 1 down-regulated 11β-HSD1 expression in fibroblasts and myeloid cells, while glucose, fatty acids, TNF-α and IL-1β did not affect it. Insulin exerted its inhibitory effect on 11β-HSD1 expression by activating PI3-kinase/Akt-signalling. Consequently, the inhibitory effect of insulin is attenuated in fibroblasts from insulin-resistant db/db mice. We conclude that insulin resistance in obesity and diabetes prevents the down-regulation of 11β-HSD1, leading to elevated endogenous GC levels in diabetic skin, which could contribute to impaired wound healing in patients with DM.
Impact of Tranexamic Acid on Chondrocytes and Osteogenically Differentiated Human Mesenchymal Stromal Cells (hMSCs) In Vitro
Authors: Wagenbrenner Mike , Heinz Tizian , Horas Konstantin , Jakuscheit Axel , Arnholdt Joerg , Mayer-Wagner Susanne , Rudert Maximilian , Holzapfel Boris M. , Weißenberger Manuel
Published: November 2020

The topical application of tranexamic acid (TXA) helps to prevent post-operative blood loss in total joint replacements. Despite these findings, the effects on articular and periarticular tissues remain unclear. Therefore, this in vitro study examined the effects of varying exposure times and concentrations of TXA on proliferation rates, gene expression and differentiation capacity of chondrocytes and human mesenchymal stromal cells (hMSCs), which underwent osteogenic differentiation. Chondrocytes and hMSCs were isolated and multiplied in monolayer cell cultures. Osteogenic differentiation of hMSCs was induced for 21 days using a differentiation medium containing specific growth factors. Cell proliferation was analyzed using ATP assays. Effects of TXA on cell morphology were examined via light microscopy and histological staining, while expression levels of tissue-specific genes were measured using semiquantitative RT-PCR. After treatment with 50 mg/mL of TXA, a decrease in cell proliferation rates was observed. Furthermore, treatment with concentrations of 20 mg/mL of TXA for at least 48 h led to a visible detachment of chondrocytes. TXA treatment with 50 mg/mL for at least 24 h led to a decrease in the expression of specific marker genes in chondrocytes and osteogenically differentiated hMSCs. No significant effects were observed for concentrations beyond 20 mg/mL of TXA combined with exposure times of less than 24 h. This might therefore represent a safe limit for topical application in vivo. Further research regarding in vivo conditions and effects on hMSC functionality are necessary to fully determine the effects of TXA on articular and periarticular tissues.
Prognostic Value of Different CMR-Based Techniques to Assess Left Ventricular Myocardial Strain in Takotsubo Syndrome
Authors: Stiermaier Thomas , Busch Kira , Lange Torben , Pätz Toni , Meusel Moritz , Backhaus Sören J. , Frydrychowicz Alex , Barkhausen Jörg , Gutberlet Matthias , Thiele Holger , Schuster Andreas , Eitel Ingo
Published: November 2020

Cardiac magnetic resonance (CMR)-derived left ventricular (LV) global longitudinal strain (GLS) provides incremental prognostic information on various cardiovascular diseases but has not yet been investigated comprehensively in patients with Takotsubo syndrome (TS). This study evaluated the prognostic value of feature tracking (FT) GLS, tissue tracking (TT) GLS, and fast manual long axis strain (LAS) in 147 patients with TS, who underwent CMR at a median of 2 days after admission. Long-term mortality was assessed 3 years after the acute event. In contrast to LV ejection fraction and tissue characteristics, impaired FT-GLS, TT-GLS and fast manual LAS were associated with adverse outcome. The best cutoff points for the prediction of long-term mortality were similar with all three approaches: FT-GLS −11.28%, TT-GLS −11.45%, and fast manual LAS −10.86%. Long-term mortality rates were significantly higher in patients with FT-GLS > −11.28% (25.0% versus 9.8%; p = 0.029), TT-GLS > −11.45% (20.0% versus 5.4%; p = 0.016), and LAS > −10.86% (23.3% versus 6.6%; p = 0.014). However, in multivariable analysis, diabetes mellitus (p = 0.001), atrial fibrillation (p = 0.001), malignancy (p = 0.006), and physical triggers (p = 0.006) outperformed measures of myocardial strain and emerged as the strongest, independent predictors of long-term mortality in TS. In conclusion, CMR-based longitudinal strain provides valuable prognostic information in patients with TS, regardless of the utilized technique of assessment. Long-term mortality, however, is mainly determined by comorbidities.
Role of Thromboelastography as an Early Predictor of Disseminated Intravascular Coagulation in Patients with Septic Shock
Authors: Kim Sang Min , Kim Sang-Il , Yu Gina , Kim June-Sung , Hong Seok In , Chae Bora , Shin Yo Sep , Kim Youn Jung , Jang Seongsoo , Kim Won Young
Published: November 2020

(1) Background: The currently proposed criteria for diagnosing overt disseminated intravascular coagulation (DIC) are not suitable for early detection of DIC. Thromboelastography (TEG) rapidly provides a comprehensive assessment of the entire coagulation process and is helpful as a guide for correcting consumptive coagulopathy in sepsis-induced DIC. This study aimed to investigate the role of TEG in the prediction of DIC in patients with septic shock. (2) Methods: TEG was conducted prospectively in 1294 patients with septic shock at the emergency department (ED) between January 2016 and December 2019. After exclusion of 405 patients with “do not attempt resuscitation” orders, those refusing enrollment, and those developing septic shock after ED presentation, 889 patients were included. DIC was defined as an International Society on Thrombosis and Hemostasis score ≥ 5 points within 24 h. (3) Results: Of the 889 patients with septic shock (mean age 65.6 ± 12.7 years, 58.6% male), 158 (17.8%) developed DIC. TEG values, except lysis after 30 min, were significantly different between the DIC and non-DIC groups. Among the TEG values, the maximal amplitude (MA) had the highest discriminating power for DIC, with an area under the curve of 0.814. An MA < 60 indicated DIC with 79% sensitivity, 73% specificity, and 94% negative predictive value. Based on multivariable analysis, MA < 60 was an independent predictor of DIC (odds ratio 5.616 (95% confidence interval: 3.213–9.818)). (4) Conclusions: In patients with septic shock, the MA value in TEG could be a valuable tool for early prediction of DIC.
Dosimetric Comparison of Intensity-Modulated Radiotherapy, Volumetric Modulated Arc Therapy and Hybrid Three-Dimensional Conformal Radiotherapy/Intensity-Modulated Radiotherapy Techniques for Right Breast Cancer
Authors: Liu Yi-Chi , Chang Hung-Ming , Lin Hsin-Hon , Lu Chia-Chun , Lai Lu-Han
Published: November 2020

This study aimed to compare different types of right breast cancer radiotherapy planning techniques and to estimate the whole-body effective doses and the critical organ absorbed doses. The three planning techniques are intensity-modulated radiotherapy (IMRT), volumetric modulated arc therapy (VMAT; two methods) and hybrid 3D-CRT/IMRT (three-dimensional conformal radiotherapy/intensity-modulated radiotherapy). The VMAT technique includes two methods to deliver a dose: non-continuous partial arc and continuous partial arc. A thermoluminescent dosimeter (TLD) is placed in the RANDO phantom to estimate the organ absorbed dose. Each planning technique applies 50.4 Gy prescription dose and treats critical organs, including the lung and heart. Dose-volume histogram was used to show the planning target volume (V95%), homogeneity index (HI), conformity index (CI), and other optimized indices. The estimation of whole-body effective dose was based on the International Commission on Radiation Protection (ICRP) Publication 60 and 103. The results were as follows: Continuous partial arc and non-continuous partial arc showed the best CI and HI. The heart absorbed doses in the continuous partial arc and hybrid 3D-CRT/IMRT were 0.07 ± 0.01% and 0% (V5% and V10%, respectively). The mean dose of the heart was lowest in hybrid 3D-CRT/IMRT (1.47 Gy ± 0.02). The dose in the left contralateral lung (V5%) was lowest in continuous partial arc (0%). The right ipsilateral lung average dose and V20% are lowest in continuous partial arc. Hybrid 3D-CRT/IMRT has the lowest mean dose to contralateral breast (organs at risk). The whole-body effective doses for ICRP-60 and ICRP-103 were highest in continuous partial arc (2.01 Sv ± 0.23 and 2.89 Sv ± 0.15, respectively). In conclusion, the use of VMAT with continuous arc has a lower risk of radiation pneumonia, while hybrid 3D-CRT/IMRT attain lower secondary malignancy risk and cardiovascular complications.
Effects of Land Cover Changes on Net Primary Productivity in the Terrestrial Ecosystems of China from 2001 to 2012
Authors: Li Hanwei , Ding Juhua , Zhang Jiang , Yang Zhenan , Yang Bin , Zhu Qiuan , Peng Changhui
Published: November 2020

The 2001–2012 MODIS MCD12Q1 land cover data and MOD17A3 NPP data were used to calculate changes in land cover in China and annual changes in net primary productivity (NPP) during a 12-year period and to quantitatively analyze the effects of land cover change on the NPP of China’s terrestrial ecosystems. The results revealed that during the study period, no changes in land cover type occurred in 7447.31 thousand km2 of China, while the area of vegetation cover increased by 160.97 thousand km2 in the rest of the country. Forest cover increased to 20.91%, which was mainly due to the conversion of large areas of savanna (345.19 thousand km2) and cropland (178.96 thousand km2) to forest. During the 12-year study period, the annual mean NPP of China was 2.70 PgC and increased by 0.25 PgC, from 2.50 to 2.75 PgC. Of this change, 0.21 PgC occurred in areas where there was no land cover change, while 0.04 PgC occurred in areas where there was land cover change. The contributions of forest and cropland to NPP exhibited increasing trends, while the contributions of shrubland and grassland to NPP decreased. Among these land cover types, the contributions of forest and cropland to the national NPP were the greatest, accounting for 40.97% and 27.95%, respectively, of the annual total NPP. There was no significant correlation between changes in forest area and changes in total annual NPP (R2 < 0.1), while the correlation coefficient for changes in cropland area and total annual NPP was 0.48. Additionally, the area of cropland converted to other land cover types was negatively correlated with the changes in NPP, and the loss of cropland caused a reduction in the national NPP.
Land Financialization, Uncoordinated Development of Population Urbanization and Land Urbanization, and Economic Growth: Evidence from China
Authors: Ji Yunyang , Guo Xiaoxin , Zhong Shihu , Wu Lina
Published: November 2020

In recent years, it has become common practice for Chinese local governments to inject land assets into financing platform companies and use them as mortgage or credit guarantees to obtain bank loans and issue urban investment bonds, which is known as “land financialization”. This study investigates the impact and mechanism of land financialization on the uncoordinated development of population urbanization and land urbanization in China. Theoretical analysis and empirical analysis results based on the data of prefecture-level cities in China from 2006 to 2015 demonstrate that land financialization by local governments is a significant cause of the uncoordinated development of population urbanization and land urbanization, and the pressure of urban economic development will strengthen this negative impact. Extended analysis further reveals that in areas where population urbanization and land urbanization are uncoordinated, land financialization, while promoting urban spatial expansion, will lower land use efficiency and have an inverted U-shaped influence on economic growth due to a weak agglomeration effect. The above conclusion shows that urbanization driven by debt-based investment is unsustainable. Efforts should be made to establish a financialization system that propels sound urbanization and to build a stable input linkage between land financialization and the supply of urban public service.
Cross-Disciplinary Genomics Approaches to Studying Emerging Fungal Infections
Authors: Ghosh Pria N. , Brookes Lola M. , Edwards Hannah M. , Fisher Matthew C. , Jervis Phillip , Kappel Dana , Sewell Thomas R. , Shelton Jennifer M.G. , Skelly Emily , Rhodes Johanna L.
Published: November 2020

Emerging fungal pathogens pose a serious, global and growing threat to food supply systems, wild ecosystems, and human health. However, historic chronic underinvestment in their research has resulted in a limited understanding of their epidemiology relative to bacterial and viral pathogens. Therefore, the untargeted nature of genomics and, more widely, -omics approaches is particularly attractive in addressing the threats posed by and illuminating the biology of these pathogens. Typically, research into plant, human and wildlife mycoses have been largely separated, with limited dialogue between disciplines. However, many serious mycoses facing the world today have common traits irrespective of host species, such as plastic genomes; wide host ranges; large population sizes and an ability to persist outside the host. These commonalities mean that -omics approaches that have been productively applied in one sphere and may also provide important insights in others, where these approaches may have historically been underutilised. In this review, we consider the advances made with genomics approaches in the fields of plant pathology, human medicine and wildlife health and the progress made in linking genomes to other -omics datatypes and sets; we identify the current barriers to linking -omics approaches and how these are being underutilised in each field; and we consider how and which -omics methodologies it is most crucial to build capacity for in the near future.