bluuebunny/crossref_metadata_2025 huggingface dataset:
Brackish and Hypersaline Lakes as Potential Reservoir for Enzymes Involved in Decomposition of Organic Materials on Frescoes
Authors: Gomoiu Ioana , Cojoc Roxana , Ruginescu Robert , Neagu Simona , Enache Madalin , Maria Gabriel , Dumbrăvician Maria , Olteanu Ioana , Rădvan Roxana , Ratoiu Lucian-Cristian , Atanassova Victoria , Ghervase Luminița
Published: September 2022

This study highlights the decomposing role through the hydrolytic activities of fungi isolated from natural environments represented by brackish and hypersaline lakes in Romania. Novel strains belonging to the Penicillium, Aspergillus, and Emericellopsis genera were isolated and screened for the ability to produce extracellular hydrolytic enzymes, i.e., proteases, lipases, amylases, cellulases, xylanases, and pectinases. According to salt requirements, they were classified as moderate halophilic and halotolerant strains. Agar plate-based assays with Tween 80, slide cultures with organic deposits, and quantitative evaluation allowed the selection of Aspergillus sp. BSL 2-2, Penicillium sp. BSL 3-2, and Emericellopsis sp. MM2 as potentially good decomposers of organic matter not only in lakes but also on deposits covering the mural paintings. Experiments performed on painted experimental models revealed that only Penicillium sp. BSL 3-2 decomposed Paraloid B72, transparent dispersion of casein, beeswax, sunflower oil, and soot. Moreover, using microscopic, spectroscopic, and imaging methods, it was proved the efficiency of Penicillium sp. BSL 3-2 for decomposition of organic deposits artificially applied on frescoes fragments.
Conversion of Syngas from Entrained Flow Gasification of Biogenic Residues with Clostridium carboxidivorans and Clostridium autoethanogenum
Authors: Rückel Anton , Oppelt Anne , Leuter Philipp , Johne Philipp , Fendt Sebastian , Weuster-Botz Dirk
Published: September 2022

Synthesis gas fermentation is a microbial process, which uses anaerobic bacteria to convert CO-rich gases to organic acids and alcohols and thus presents a promising technology for the sustainable production of fuels and platform chemicals from renewable sources. Clostridium carboxidivorans and Clostridium autoethanogenum are two acetogenic bacteria, which have shown their high potential for these processes by their high tolerance toward CO and in the production of industrially relevant products such as ethanol, 1-butanol, 1-hexanol, and 2,3-butanediol. A promising approach is the coupling of gasification of biogenic residues with a syngas fermentation process. This study investigated batch processes with C. carboxidivorans and C. autoethanogenum in fully controlled stirred-tank bioreactors and continuous gassing with biogenic syngas produced by an autothermal entrained flow gasifier on a pilot scale >1200 °C. They were then compared to the results of artificial gas mixtures of pure gases. Because the biogenic syngas contained 2459 ppm O2 from the bottling process after gasification of torrefied wood and subsequent syngas cleaning for reducing CH4, NH3, H2S, NOX, and HCN concentrations, the oxygen in the syngas was reduced to 259 ppm O2 with a Pd catalyst before entering the bioreactor. The batch process performance of C. carboxidivorans in a stirred-tank bioreactor with continuous gassing of purified biogenic syngas was identical to an artificial syngas mixture of the pure gases CO, CO2, H2, and N2 within the estimation error. The alcohol production by C. autoethanogenum was even improved with the purified biogenic syngas compared to reference batch processes with the corresponding artificial syngas mixture. Both acetogens have proven their potential for successful fermentation processes with biogenic syngas, but full carbon conversion to ethanol is challenging with the investigated biogenic syngas.
Comparative Fatty Acid Compositional Profiles of Rhodotorula toruloides Haploid and Diploid Strains under Various Storage Conditions
Authors: Zhang Yue , Kamal Rasool , Li Qing , Yu Xue , Wang Qian , Zhao Zongbao Kent
Published: September 2022

Microbial-based fatty acids (FAs), biofuels and oleochemicals are potential alternatives to fossil fuels and other non-renewable resources. Rhodotorula toruloides (formerly Rhodosporidium toruloides) is a basidiomycetous oleaginous yeast, and cells of the wild-type diploids can accumulate lipids to over 70 wt% on a dry cell weight basis in nutrient-limited conditions. Meanwhile, several haploid strains have been applied as hosts for producing high-value fatty acid derivatives through genetic modification and metabolic engineering. However, the differences in fatty acid compositional profiles and their stability between diploid and haploid strains remain unknown in this oleaginous yeast. Here, we grew a haploid strain R. toruloides NP11 and its parental diploid strain R. toruloides CGMCC 2.1389 (4#) under identical conditions and compared the profiles in terms of cell growth, lipid production, fatty acid compositions of lipids as well as storage stability of fatty acid methyl esters (FAMEs). It was found that lipids from R. toruloides composed of fatty acids in terms of chain length ranged from short-chain FAs (C6–C9) to very long-chain FAs (VLCFAs, C20–C24) and some odd-chain FAs (C15 and C17), while long-chain fatty acids (C14–C18) were the most abundant ones. In addition, NP11 produced a little more (1 wt%) VLCFAs than that of the diploid strain 4#. Moreover, no major changes were found for FAMEs being held under varied storage conditions, suggesting that FAMEs samples were stable and robust for fatty acid compositional analysis of microbial lipids. This work revealed the fatty acid profiles of lipids from R. toruloides haploid and diploid strains, and their stability under various storage conditions. The information is valuable for reliable assessment of fatty acid compositions of lipids from oleaginous yeasts and related microbial cell factories.
Wildfire Risk in the Complex Terrain of the Santa Barbara Wildland–Urban Interface during Extreme Winds
Authors: Zigner Katelyn , Carvalho Leila M. V. , Jones Charles , Benoit John , Duine Gert-Jan , Roberts Dar , Fujioka Francis , Moritz Max , Elmquist Nic , Hazard Rob
Published: September 2022

Each year, wildfires ravage the western U.S. and change the lives of millions of inhabitants. Situated in southern California, coastal Santa Barbara has witnessed devastating wildfires in the past decade, with nearly all ignitions started by humans. Therefore, estimating the risk imposed by unplanned ignitions in this fire-prone region will further increase resilience toward wildfires. Currently, a fire-risk map does not exist in this region. The main objective of this study is to provide a spatial analysis of regions at high risk of fast wildfire spread, particularly in the first two hours, considering varying scenarios of ignition locations and atmospheric conditions. To achieve this goal, multiple wildfire simulations were conducted using the FARSITE fire spread model with three ignition modeling methods and three wind scenarios. The first ignition method considers ignitions randomly distributed in 500 m buffers around previously observed ignition sites. Since these ignitions are mainly clustered around roads and trails, the second method considers a 50 m buffer around this built infrastructure, with ignition points randomly sampled from within this buffer. The third method assumes a Euclidean distance decay of ignition probability around roads and trails up to 1000 m, where the probability of selection linearly decreases further from the transportation paths. The ignition modeling methods were then employed in wildfire simulations with varying wind scenarios representing the climatological wind pattern and strong, downslope wind events. A large number of modeled ignitions were located near the major-exit highway running north–south (HWY 154), resulting in more simulated wildfires burning in that region. This could impact evacuation route planning and resource allocation under climatological wind conditions. The simulated fire areas were smaller, and the wildfires did not spread far from the ignition locations. In contrast, wildfires ignited during strong, northerly winds quickly spread into the wildland–urban interface (WUI) toward suburban and urban areas.
The Socioeconomic Impact of Coastal Environment Changes on Fishing Communities and Adaptation Strategies
Authors: Hsiao Yao-Jen
Published: September 2022

Oysters are one of the main aquatic products produced in Taiwan. However, because of the gradual changes in the environment of the Waisanding Sandbar, the oyster farming environment along the coast of Chiayi is shrinking, leading to greater operational risk and uncertainty. This study adopted a three-stage method to investigate the socioeconomic uncertainty that fishing communities face regarding environmental changes and to understand the environmental risk and fishery management awareness of different stakeholders as well as their views on adaptation strategies. In-depth interviews were used in the first stage. In Stage 2, two-round focus group interviews were conducted to organize views on the environment and climate, the oyster industry and management, and adaptation strategies. In the final stage, the AHP method was adopted to analyze the opinions of different stakeholders. The results showed factors affecting environmental change, oyster industry, and management among different stakeholders as well as the cognitive differences within the corresponding adaptation strategies. Therefore, socioeconomic and ecology complexities and uncertainties should be considered for enhancing social capital and promoting risk communication, more diverse social–ecological system data to assist fishery governance, and oyster industry development and adaptation strategies.
Effect of Replacing Fishmeal with Plukenetia volubilis Cake on Growth, Digestive Enzymes, and Body Composition in Whiteleg Shrimp (Litopenaeus vannamei)
Authors: Renteria Patricio , Vizcaíno Antonio Jesús , Sánchez-Muros María José , Santacruz-Reyes Roberto A. , Saez María Isabel , Fabrikov Dmitri , Barroso Fernando G. , Vargas-García María del Carmen
Published: September 2022

A feeding trial was carried out on a shrimp farm located in the Santa Rosa province of El Oro, Ecuador, with four isonitrogenous and isolipidic experimental diets, designed with increasing levels of substitution of fish meal by P. voluvilis, (D-0), 15% (D-15), 25% (D-25), and 50% (D-50). The obtained results indicated that the 50% replacement with P. volubilis in practical diets had no noticeable negative effects on the growth performance of L. vanameii juveniles. The total weight of shrimps fed with 25% and 50% diets (16.04 g and 16.72 g, respectively) and the abdomen weight (10.32 g) of shrimps fed with the D-50 diet were high, with significant differences regarding those fed with the D-0 diet. No adverse effects on muscle composition were found. Significant differences were observed only in groups D-25 (for chymotrypsin) and D-50 (chymotrypsin and alkaline protease). Trypsin and amylase activity was not affected by the inclusion of P. volubilis. The results of this experiment indicated that P. volubilis cake is a possible alternative to fish meal in shrimp feeding; however, it should be studied in more depth to establish the maximum replacement percentage and to identify the adequate treatments to eliminate antinutritional factors.
Computational Inertial Microfluidics: Optimal Design for Particle Separation
Authors: Saha Suvash C. , Francis Isabella , Nassir Tanya
Published: September 2022

Following the emergence of many blood transfusion-associated diseases, novel passive cell separation technologies, such as microfluidic devices, are increasingly designed and optimized to separate red blood cells (RBCs) and white blood cells (WBCs) from whole blood. These systems allow for the rapid diagnosis of diseases without relying on complicated and expensive hematology instruments such as flow microscopes, coagulation analyzers, and cytometers. The inertia effect and the impact of intrinsic hydrodynamic forces, the Dean drag force (FD), and the inertial lift force (FL) on the migration of particles within curved and complex confined channels have been explored theoretically, computationally, and experimentally. This study aimed to optimize the dimensions of a microfluidic channel for fast particle propagation and separation. Several spiral geometries with different cross-sections were tested using computational fluid dynamics (CFD) to separate two particle types representing RBCs and WBCs. The chosen three geometries consist of a single inlet, two outlets, and three spiral turns, each having a different cross-sectional height (120, 135, and 150 µm). Particle separation was successfully achieved in the 135 µm-height microchannel, while other microchannels demonstrated mixed particle types at the outlets.
Integration within Fluid Dynamic Solvers of an Advanced Geometric Parameterization Based on Mesh Morphing
Authors: Cella Ubaldo , Patrizi Daniele , Porziani Stefano , Virdung Torbjörn , Biancolini Marco Evangelos
Published: September 2022

Numerical optimization procedures are one of the most powerful approaches with which to support design processes. Their implementation, nevertheless, involves several conceptual and practical complexities. One of the key points relates to the geometric parameterization technique to be adopted and its coupling with the numerical solver. This paper describes the setup of a procedure in which the shape parameterization, based on mesh morphing, is integrated into the analysis tool, accessing the grid nodes directly within the solver environment. Such a coupling offers several advantages in terms of robustness and computational time. Furthermore, the ability to morph the mesh “on the fly” during the computation, without heavy Input/Output operations, extends the solver’s capability to evaluate multidisciplinary phenomena. The procedure was preliminary tested on a simple typical shape optimization problem and then applied to a complex setup of an industrial case: the identification of the shape of a Volvo side-view mirror that minimizes the accumulation of water on the lens of a camera mounted beneath.
Identification and Evaluation of Microplastics from Tea Filter Bags Based on Raman Imaging
Authors: Mei Tingna , Wang Jiahua , Xiao Xiaofeng , Lv Jingwen , Li Qiaocong , Dai Huang , Liu Xiaodan , Pi Fuwei
Published: September 2022

Microplastic (MP) contamination is a public issue for the environment and for human health. Plastic-based food filter bags, including polyethylene terephthalate, polypropylene, nylon 6 (NY6), and polyethylene, are widely used for soft drink sub-packaging, increasing the risk of MPs in foods and the environment. Three types of commercially available filter bags, including non-woven and woven bags, were collected, and MPs released after soaking were mapped using Raman imaging combined with chemometrics. Compared with peak area imaging at a single characteristic peak, Raman imaging combined with direct classical least squares calculation was more efficient and reliable for identifying MP features. Up to 94% of the bags released MPs after soaking, and there was no significant correlation with soaking conditions. Most MPs were tiny fragments and particles, and a few were fibrous MPs 620–840 μm in size. Woven NY6 filter bags had the lowest risk of releasing MPs. Source exploration revealed that most MPs originated from fragments and particles adsorbed on the surface of bags and strings. The results of this study are applicable to filter bag risk assessment and provide scientific guidance for regulating MPs in food.
Recent Advances and Applications in Starch for Intelligent Active Food Packaging: A Review
Authors: Liu Dandan , Zhao Pei , Chen Jinyu , Yan Yali , Wu Zijian
Published: September 2022

At present, the research and innovation of packaging materials are in a period of rapid development. Starch, a sustainable, low-cost, and abundant polymer, can develop environmentally friendly packaging alternatives, and it possesses outstanding degradability and reproducibility in terms of improving environmental issues and reducing oil resources. However, performance limitations, such as less mechanical strength and lower barrier properties, limit the application of starch in the packaging industry. The properties of starch-based films can be improved by modifying starch, adding reinforcing groups, or blending with other polymers. It is of significance to study starch as an active and intelligent packaging option for prolonging shelf life and monitoring the extent of food deterioration. This paper reviews the development of starch-based films, the current methods to enhance the mechanical and barrier properties of starch-based films, and the latest progress in starch-based activity, intelligent packaging, and food applications. The potential challenges and future development directions of starch-based films in the food industry are also discussed.
Assessment of Textural Properties of Puffed Corn Cakes during Storage at Different Relative Humidity
Authors: Sanchez-Jimenez Virginia , Gomez Alvarez-Arenas Tomas E. , Rincón Marta , Benedito Jose , Garcia-Perez Jose V.
Published: September 2022

Moisture adsorption is considered a critical factor during production and shelf-life of puffed corn cakes (PCC). This study aims to develop and validate an instrumental method and a mathematical model for the characterization of the textural modifications caused by the moisture adsorption in PCC. For that purpose, PCC were stored at different relative humidities to achieve a wide range of water activities (from 0.1 to 0.8 at 22 ± 1 °C). A flexion–compression test was successfully validated in order to characterize the average textural properties of a PCC batch. A mathematical model considering consecutive elastic and plastic zones satisfactorily fitted (average VAR 99.65% and MRE 3.29%) the average stress–strain profiles of PCC and reported useful textural parameters, such as the deformability modulus (E), critical strain (εc), and n curvature parameter. The structural modifications caused by moisture adsorption led to the reduction in E and n and the increase in εc. Even minor changes on the PCC moisture content involve remarkable modifications of the textural properties, which has to be considered for industry and retail distribution.
Progress in the Application of Food-Grade Emulsions
Authors: Jie Yilin , Chen Fusheng
Published: September 2022

The detailed investigation of food-grade emulsions, which possess considerable structural and functional advantages, remains ongoing to enhance our understanding of these dispersion systems and to expand their application scope. This work reviews the applications of food-grade emulsions on the dispersed phase, interface structure, and macroscopic scales; further, it discusses the corresponding factors of influence, the selection and design of food dispersion systems, and the expansion of their application scope. Specifically, applications on the dispersed-phase scale mainly include delivery by soft matter carriers and auxiliary extraction/separation, while applications on the scale of the interface structure involve biphasic systems for enzymatic catalysis and systems that can influence substance digestion/absorption, washing, and disinfection. Future research on these scales should therefore focus on surface-active substances, real interface structure compositions, and the design of interface layers with antioxidant properties. By contrast, applications on the macroscopic scale mainly include the design of soft materials for structured food, in addition to various material applications and other emerging uses. In this case, future research should focus on the interactions between emulsion systems and food ingredients, the effects of food process engineering, safety, nutrition, and metabolism. Considering the ongoing research in this field, we believe that this review will be useful for researchers aiming to explore the applications of food-grade emulsions.
Antimicrobial and Antioxidant Effects of Kappa-Carrageenan Coatings Enriched with Cinnamon Essential Oil in Pork Meat
Authors: He Shoukui , Wang Yifei
Published: September 2022

Fresh pork is susceptible to microbial contamination and lipid oxidation, which leads to food safety and quality issues. This study aimed to develop a kappa-carrageenan (KC) coating embedded with cinnamon essential oil (CEO) for antimicrobial and antioxidant purposes in pork meat. The uncoated controls and coated samples were subjected to microbial (total viable count, lactic acid bacteria, and H2S-producing bacteria), chemical (DPPH and pH), and physical (surface color) analyses during refrigerated storage at 4 °C for 7 days. It was observed that KC coatings exhibited a better preservation effect on pork meat after the addition of CEO. The KC–CEO coatings were effective in retarding the growth of total viable count, lactic acid bacteria, and H2S-producing bacteria. In a DPPH test, the level of lipid oxidation in pork meat was also significantly (p < 0.05) reduced by the KC–CEO coatings. Furthermore, these coatings displayed pronounced activity in inhibiting the adverse alterations of pH value and surface color. Practically, KC–CEO-coated samples still exhibited an attractive bright red color at the end of refrigerated storage. Taken together, the developed KC–CEO coatings exerted pronounced antimicrobial and antioxidant activities in pork, thus providing a potential approach to preserving perishable meat.
Conversion of Pulse Protein Foam-Templated Oleogels into Oleofoams for Improved Baking Application
Authors: Mohanan Athira , Harrison Kim , Cooper David M. L. , Nickerson Michael T. , Ghosh Supratim
Published: September 2022

The food industry has long been searching for an efficient replacement for saturated-fatty-acid-rich fats for baking applications. Although oleogels have been considered a potential alternative for saturated and trans fats, their success in food application has been poor. The present study explored the use of oleofoams obtained by whipping the pulse protein foam-templated oleogels for cake baking. Oleogels were prepared at room temperature by adding canola oil containing high-melting monoglyceride (MAG) or candelilla wax (CW) to the freeze-dried pea or faba bean protein-stabilized foams. Oleogels were then whipped to create the oleofoams; however, only the oleogels containing MAG could form oleofoams. CW-oleogel could not form any oleofoam. The most stable oleofoams with the highest overrun, stability, and storage modulus were obtained from 3% MAG+pulse protein foam-templated oleogels. The MAG plus protein foam-templated oleogels showed smaller and more packed air bubbles than MAG-only oleofoam, which was ascribed to the protein’s ability to stabilize air bubbles and provide a network in the continuous oil phase to restrict air bubble movement. A novel batter preparation method for oleofoam was developed to increase air bubble incorporation. The X-ray microtomography images of the cakes showed a non-homogeneous distribution of larger air bubbles in the oleofoam cake compared to the shortening cake although their total porosity was not much different. The oleofoam cakes made with the new method yielded similar hardness and chewiness compared to the shortening cakes. By improving rheology and increasing air incorporation in the batter, high-quality cakes can be obtained with MAG-containing oleofoams made from pulse protein foam-templated oleogels.
Randomized Pilot Study on the Effects of Sarcocornia as a Salt Substitute in Arterial Blood Pressure and Vascular Function in Healthy Young Adults
Authors: Pereira Telmo , Caldeira Andreia Torres , Caseiro Armando , Osório Nádia , da Silva Aida Moreira , Barroca Maria João
Published: September 2022

Previous studies have shown that excessive salt intake is strongly associated with high blood pressure (HT), vascular dysfunction, and the overall risk of cardiovascular diseases. The aim of this study was to evaluate Sarcocornia effectiveness as a salt substitute, addressing its effect on cardiovascular function in healthy young individuals. Thirty healthy participants, aged 18 to 26 years, were randomized into two groups: the control group (CG) and the intervention group (IG). The IG used Sarcocornia powder as a salt substitute for cooking, and the CG used regular salt, during a period of 1 month. A baseline evaluation was performed before the participants started the intervention phase, and was repeated after a 30-day intervention period. Each evaluation included blood pressure (BP) measurement, carotid-femoral pulse wave velocity (PWV), and carotid pulse wave analysis (PWA), and blood samples were also collected for analysis. Sodium excretion was measured at baseline and after intervention through spot urine collection and analysis, a method suitable for this population but with known limitations. Baseline parameters were similar between groups and were within the normal range. Sodium excretion remained unchanged in the two evaluations in the CG, but significantly decreased after intervention in the IG. The reduction in sodium excretion in the IG was followed by a significant reduction in brachial and aortic systolic (SBP) and diastolic blood pressure (DBP), and also in PWV. No significant changes were observed in the CG in terms of cardiovascular parameters. This preliminary study conveys positive results in favor of Sarcocornia as a dietary substitute for regular salt, providing added evidence of the negative cardiovascular effects of high salt intake in young and healthy adults.
Does the Rearing Management following by Charolais Cull Cows Influence the Qualities of Carcass and Beef Meat?
Authors: Soulat Julien , Picard Brigitte , Monteils Valérie
Published: September 2022

This study characterized, for the first time, the rearing managements (from birth to slaughter) applied throughout the cull cows’ life and observed the effect of these managements on the carcass and meat properties. From the individual data of 371 Charolais cull cows, three rearing managements were defined and characterized with 60 rearing factors. The results showed that the rearing managements had low effects on the carcass and meat properties. For the carcass traits, only the carcass weight, and fat and longissimus (LM) colors at the level of the sixth rib were impacted. Concerning the meat, only the red color intensity, the fat aroma, the flavor intensity and persistence were affected. According to our results, this study confirmed that it is possible to produce carcass or meat with similar properties; consequently, it is difficult to favor a rearing management. However, to manage jointly both carcass and meat qualities, trade-offs are needed.
Two-Dimensional Liquid Chromatography Method for the Determination of Gelsemium Alkaloids in Honey
Authors: Ma Xiao , Zuo Meng-Ting , Qi Xue-Jia , Wang Zi-Yuan , Liu Zhao-Ying
Published: September 2022

Toxic Chinese medicine residues in honey pose a serious threat to consumer health. Gelsemium is one of the nine ancient poisons, making the whole plant virulent. The residue of Gelsemium alkaloid in honey causes poisoning from time to time. Therefore, it is very important to establish a method for the detection of Gelsemium alkaloids in honey. In this study, a method of solid phase extraction (SPE) with two-dimensional liquid chromatography (2D-LC) was developed for the first time for the simultaneous determination of Gelsemium alkaloids in honey, including gelsemine, koumine and humantenmine. First, the honey samples were purified by a PRS cation exchange column and extracted with 5% ammoniated methanol. Then, we verified the methodological indicators, which were in line with the Codex Guideline requirements. The verification results are as follows: matrix-matched calibrations indicated that the correlation coefficients were higher than 0.998. The recovery was in the range of 81%–94.2% with an intraday precision (RSD) of ≤5.0% and interday RSD of ≤3.8%. The limit of detection for the three alkaloids was 2 ng/g. The limits of quantification for gelsemine and koumine were 5 ng/g, and humantenmine was 20 ng/g. This method can be applied to the monitoring of Gelsemium alkaloids in honey.
The Volatile Profile of Brussels Sprouts (Brassica oleracea Var. gemmifera) as Affected by Pulsed Electric Fields in Comparison to Other Pretreatments, Selected to Steer (Bio)Chemical Reactions
Authors: Delbaere Sophie M. , Bernaerts Tom , Vangrunderbeek Mirte , Vancoillie Flore , Hendrickx Marc E. , Grauwet Tara , Van Loey Ann M.
Published: September 2022

Pulsed electric fields (PEF) at low field strength is considered a non-thermal technique allowing membrane permeabilization in plant-based tissue, hence possibly impacting biochemical conversions and the concomitant volatile profile. Detailed studies on the impact of PEF at low field strength on biochemical conversions in plant-based matrices are scarce but urgently needed to provide the necessary scientific basis allowing to open a potential promising field of applications. As a first objective, the effect of PEF and other treatments that aim to steer biochemical conversions on the volatile profile of Brussels sprouts was compared in this study. As a second objective, the effect of varying PEF conditions on the volatile profile of Brussels sprouts was elucidated. Volatile fingerprinting was used to deduce whether and which (bio)chemical reactions had occurred. Surprisingly, PEF at 1.01 kV/cm and 2.7 kJ/kg prior to heating was assumed not to have caused significant membrane permeabilization since similar volatiles were observed in the case of only heating, as opposed to mixing. A PEF treatment with an electrical field strength of 3.00 kV/cm led to a significantly higher formation of certain enzymatic reaction products, being more pronounced when combined with an energy input of 27.7 kJ/kg, implying that these PEF conditions could induce substantial membrane permeabilization. The results of this study can be utilized to steer enzymatic conversions towards an intended volatile profile of Brussels sprouts by applying PEF.
Effects of Jet Milling Pretreatment and Esterification with Octenyl Succinic Anhydride on Physicochemical Properties of Corn Starch
Authors: Wang Lidong , Li Xiaoqiang , Gao Fei , Liu Shilin , Wu Yanchun , Liu Ying , Zhang Dongjie
Published: September 2022

(1) Background: In this study, aiming at the problems of low efficiency and high energy consumption in the esterification reaction of OSA and starch, the jet milling technology was used to pretreat corn starch and starch raw materials with different pulverization strengths were obtained by controlling the speed of the classifier. (2) Methods: The starch obtained under different classification speeds was modified by esterification with OSA. Using CLSM, FTIR, XRD, NMR, FTIR, XPS, and other technologies, the modification effect was verified, and the physical and chemical properties of J-OSA-Starch such as DSC, RVA, transparency, and emulsifying properties were determined. (3) Results: Jet milling pretreatment significantly reduced the particle size of corn starch and improved the reaction efficiency and degree of substitution during esterification with OSA. After pretreatment, the corn starch granules were broken, and the relative crystallinity was significantly reduced. CLSM, FTIR, XPS, and NMR confirmed the esterification of corn starch with OSA, which increased with increasing crushing strength. The thermodynamic properties and viscosity of J-OSA-starch decreased with the increase in the classification speed. Jet milling pretreatment enhanced the clarity, emulsifying activity, and emulsifying stability of OSA-modified corn starch. (4) Conclusions: Jet milling pretreatment can effectively increase the esterification efficiency of starch and OSA. Therefore, jet milling can be used as a pretreatment to improve the esterification of starch OSA and produce modified starch for industrial applications.
Structural Characterization and Evaluation of Interfacial Properties of Pea Protein Isolate–EGCG Molecular Complexes
Authors: Han Shuang , Cui Fengzhan , McClements David Julian , Xu Xingfeng , Ma Cuicui , Wang Yutang , Liu Xuebo , Liu Fuguo
Published: September 2022

There is increasing interest in using plant-derived proteins in foods and beverages for environmental, health, and ethical reasons. However, the inherent physicochemical properties and functional performance of many plant proteins limit their widespread application. Here, we prepared pea protein isolate (PPI) dispersions using a combined pH-shift/heat treatment method, and then, prepared PPI-epigallocatechin-3-gallate (EGCG) complexes under neutral conditions. Spectroscopy, calorimetry, molecular docking, and light scattering analysis demonstrated that the molecular complexes formed spontaneously. This was primarily ascribed to hydrogen bonds and van der Waals forces. The complexation of EGCG caused changes in the secondary structure of PPI, including the reduction in the α-helix and increase in the β-sheet and disordered regions. These changes slightly decreased the thermal stability of the protein. With the accretion of EGCG, the hydrophilicity of the complexes increased significantly, which improved the functional attributes of the protein. Optimization of the PPI-to-EGCG ratio led to the complexes having better foaming and emulsifying properties than the protein alone. This study could broaden the utilization of pea proteins as functional ingredients in foods. Moreover, protein–polyphenol complexes can be used as multifunctional ingredients, such as antioxidants or nutraceutical emulsifiers.
Effects of Two Kinds of Extracts of Cistanche deserticola on Intestinal Microbiota and Its Metabolism
Authors: Li Yilin , Zhang Yalin , Su Xiaoming , Zou Pengfei , Wang Xinyang , Chen Jie , Zhu Xuan
Published: September 2022

Cistanche deserticola belongs to the Liedang family. Known as “desert ginseng”, it has high medicinal value and plays important roles in endocrine regulation, neuroprotection, immune regulation, and other processes. Some studies have shown that single substances such as polysaccharides and phenylethanolside can affect intestinal microbiota, but few studies have studied the synergistic effect of various components in Cistanche deserticola extracts on intestinal microbiota. Therefore, in this study, through an in vitro digestion model (Changdao Moni, CDMN) combined with 16S rRNA gene amplification sequencing technology and untargeted metabolomics technology, it was found that the two extracts all had significant effects on the enteric cavity and mucosal flora. Both extracts inhibited Bacteroides in the intestinal cavity and Parabacteroides and Ruminococcus 2 in the intestinal mucosa and promoted Bifidobacterium and Prevotella in the intestinal cavity and Megasphaera in the intestinal mucosa. The aqueous extract also inhibited Phascolarctobacterium. Both extracts also significantly increased the production of short-chain fatty acids, especially butyrate. The intake of extract had significant effects on the metabolic pathways related to amino acids and lipids. Indoles were upregulated by the aqueous extract but downregulated by the alcohol extract. In addition, the extract also had a significant effect on the hemolytic phosphorus esters. In conclusion, the two kinds of extracts have different effects on intestinal microbiota and its metabolism. This study provides guiding significance for the edibility and food development of Cistanche deserticola.
Transcriptomic and Metabolomic Analyses Providing Insights into the Coloring Mechanism of Docynia delavayi
Authors: Wang Yuchang , Song Yuyang , Wang Dawei
Published: September 2022

The metabolome and transcriptome profiles of three different variations of mature Docynia delavayi fruit were synthesized to reveal their fruit color formation mechanism. A total of 787 secondary metabolites containing 149 flavonoid metabolites, most of which were flavonoids and flavonols, were identified in the three variations using ultra performance liquid chromatography- tandem mass spectrometry (UPLC-MS/MS), and we found that the secondary metabolites cyanidin-3-O-galactoside and cyanidin-3-O-glucoside were the major coloring substances in D. delavayi. This was associated with the significant upregulation of the structural genes F3H and F3′H in the anthocyanin synthesis pathway and the control genes WRKY, MYB, bZIP, bHLH, and NAC in RP. F3′H expression may play a significant role in the selection of components for anthocyanin synthesis. Our results contribute to breeding and nutritional research in D. delavayi and provide insight into metabolite studies of the anthocyanin biosynthetic pathway.
Separation of Dimethyl Sulfide from Wort by Multi-Layer Centrifugal Film Method
Authors: Dai Xiaoyong , Wang Pengyu , Wu Wei , Wang Haoyu , Xu Qing , Li Zhanyong
Published: September 2022

Installing a separation device for undesirable volatile substances represented by dimethyl sulfide (DMS) in wort boiling systems is a common way to reduce the thermal stress and maintain the beer’s flavor stability (characterized by the thiobarbituric acid (TBA) value), but most of these separation devices need to provide additional vacuum or primary thermal energy. This research shows that it can produce self-evaporation that consumes its own sensible heat when wort is in the state of turbulent film. Therefore, a new gas-liquid separation system named the multilayer centrifugal film-forming device (similar to the spinning cone column (SCC)) is proposed, which can strengthen self-evaporation through wort turbulent film and create gas phase conditions for the separation of undesirable volatile substances. The results show that up to 91.6% of the content of DMS in wort could be significantly removed by centrifugal film self-evaporation. The TBA value of wort was reduced by more than 15%, and the wort was not found to be oxidized. Compared with the traditional boiling method, the multi-layer centrifugal film-forming device can significantly save primary energy consumption and reduce energy consumption by 216.4 kJ per liter of wort during the boiling and cooling process.
Active Packaging for the Extended Shelf-Life of Meat: Perspectives from Consumption Habits, Market Requirements and Packaging Practices in China and New Zealand
Authors: Li Xin , Zhang Renyu , Hassan Mohammad Mahbubul , Cheng Zhe , Mills John , Hou Chengli , Realini Carolina E. , Chen Li , Day Li , Zheng Xiaochun , Zhang Dequan , Hicks Talia M.
Published: September 2022

Active packaging (AP) has been developed to improve the safety, quality and integrity of food, and minimise food waste, while its application in meat is scarce. This review aims to describe meat production and consumption culture in China and New Zealand to provide the context for packaging innovation requirements, focusing on the emerging opportunities for AP to be used for the improvement of the shelf-life of pre-rigor, aged, and frozen-thawed meat products. Sustainable polymers utilised in the manufacturing of AP, manufacturing techniques, the release mechanisms of actives, and legal and regulatory constraints are also discussed. Diverse market compositions and consumption cultures in China and New Zealand require different packaging solutions to extend the shelf-life of meat. AP containing antimicrobials, moisture regulating agents, and antioxidants may be used for pre-rigor, dry- and wet-aged products and in improving the quality and shelf-life of frozen-thawed meat. Further innovations using sustainably produced polymers for AP, along with incorporating active compounds of multiple functions for effectively improving meat quality and shelf-life are necessary. Challenges remain to resolve issues with scaling the technology to commercially relevant volumes as well as complying with the rigorous legal and regulatory constraints in various countries.
Isolation and Mechanistic Characterization of a Novel Zearalenone-Degrading Enzyme
Authors: Ji Jian , Yu Jian , Xu Wei , Zheng Yi , Zhang Yinzhi , Sun Xiulan
Published: September 2022

Zearalenone (ZEN) and its derivatives pose a serious threat to global food quality and animal health. The use of enzymes to degrade mycotoxins has become a popular method to counter this threat. In this study, Aspergillus niger ZEN-S-FS10 extracellular enzyme solution with ZEN-degrading effect was separated and purified to prepare the biological enzyme, FSZ, that can degrade ZEN. The degradation rate of FSZ to ZEN was 75–80% (pH = 7.0, 28 °C). FSZ can function in a temperature range of 28–38 °C and pH range of 2.0–7.0 and can also degrade ZEN derivatives (α-ZAL, β-ZOL, and ZAN). According to the enzyme kinetics fitting, ZEN has a high degradation rate. FSZ can degrade ZEN in real samples of corn flour. FSZ can be obtained stably and repeatedly from the original strain. One ZEN degradation product was isolated: FSZ−P(C18H26O4), with a relative molecular weight of 306.18 g/mol. Amino-acid-sequencing analysis revealed that FSZ is a novel enzyme (homology < 10%). According to the results of molecular docking, ZEN and ZAN can utilize their end-terminal carbonyl groups to bind FSZ residues PHE307, THR55, and GLU129 for a high-degradation rate. However, α-ZAL and β-ZOL instead contain hydroxyl groups that would prevent binding to GLU129; thus, the degradation rate is low for these derivatives.
Curcumin-Loaded Self-Assembly Constructed by Octenylsuccinate Fish (Cyprinus carpio L.) Scale Gelatin: Preparation and Characterization
Authors: Yu Xiaoyan , Li Haoxin , Wan Aida Wan Mustapha , Ren Tingyuan , Lei Zunguo , Liu Jia
Published: September 2022

Curcumin loaded octenylsuccinate fish scale gelatin (OFSG) was prepared in this study, to explore the potential of FSG for delivering hydrophobic nutrients. The effects of molecule weight (Mw, 22,677–369 g/mol) and degree of substitution (DS, 0–0.116) on the curcumin loading efficiency (CLE, μg/mL) of OFSG (6.98–26.85 mg/mL) were evaluated. The expose of interior hydrophobic groups in FSG and increased intermolecular hydrophobic area contributed to the loading of curcumin in two phases, respectively. The interaction between OFSG and curcumin showed a decreased absorption in FTIR and an increased crystallinity in XRD. The loading of curcumin into OFSG caused a significant decrease of the particle size (from 350–12,070 to 139–214 nm), PDI (from 0.584–0.659 to 0.248–0.347) and ζ-potential (−12.2 or −11.4 to −21.0 or −20.3). OFSG showed a significantly higher stability and lower release of curcumin than FSG at the end of the simulated gastrointestinal digestion. Thus, OFSG showed great potential in the construction of a carrier for hydrophobic nutrients.
New Outcomes Regarding the Existence of Hilfer Fractional Stochastic Differential Systems via Almost Sectorial Operators
Authors: Sivasankar Sivajiganesan , Udhayakumar Ramalingam
Published: September 2022

In this paper, we focus on the existence of Hilfer fractional stochastic differential systems via almost sectorial operators. The main results are obtained by using the concepts and ideas from fractional calculus, multivalued maps, semigroup theory, sectorial operators, and the fixed-point technique. We start by confirming the existence of the mild solution by using Dhage’s fixed-point theorem. Finally, an example is provided to demonstrate the considered Hilferr fractional stochastic differential systems theory.
Fundamental Properties of Late-Type Stars in Eclipsing Binaries
Authors: Morales Juan Carlos , Ribas Ignasi , Giménez Álvaro , Baroch David
Published: September 2022

Evidence from the analysis of eclipsing binary systems revealed that late-type stars are larger and cooler than predicted by models, and that this is probably caused by stellar magnetic activity. In this work, we revisit this problem taking into account the advancements in the last decade. We provide and updated a list of 32 eclipsing binary or multiple systems, including at least one star with a mass ≲0.7 M⊙ and with mass and radius measured to an accuracy better than 3%. The comparison with stellar structure and evolution theoretical models reveals an overall discrepancy of about 7% and −4% for the radius and effective temperature, respectively, and that it may be larger than previously found below the full convection boundary. Furthermore, the hypothesis of stellar activity is reinforced by the comparison of different systems with similar components. Further eclipsing binaries with accurately determined masses and radii, and with estimated activity levels, as well as the implementation of magnetic activity in theoretical models will help to improve our knowledge of low-mass stars, which are prime targets for exoplanet surveys.
(Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies
Authors: Vassiliadi Evdokia , Tsirigotis-Maniecka Marta , Symons Henry E. , Gobbo Pierangelo , Nallet Frédéric , Xenakis Aristotelis , Zoumpanioti Maria
Published: September 2022

The present work reports on the structural study of a film made of a hybrid blend of biopolymers used as an enzyme carrier. A cellulose derivative (HPMC) and chitosan (CS) were combined in order to formulate a film on which Mucor miehei lipase was immobilized. The film was successfully used as a biocatalyst; however, little is known about the structure of the system. Therefore, small-angle X-ray scattering, Fourier transform infrared spectroscopy (FTIR), optical microscopy, and scanning electron microscopy (SEM), as well as microindentation measurements, were used to shed light on the structure of the promising biocatalyst. Among the results, intermolecular hydrogen bonds were observed between the amide groups of the two polymers and the lipase. The presence of the enzyme does not seem to affect the mechanical properties of the matrix. The used film after 35 cycles of reaction seemed to be fatigued and had lost part of its humidity, explaining the reduction of the enzyme activity.
An Insight into Pasting and Rheological Behavior of Potato Starch Pastes and Gels with Whole and Ground Chia Seeds
Authors: Adamczyk Greta , Krystyjan Magdalena , Kuźniar Piotr , Kowalczewski Przemysław Łukasz , Bobel Inna
Published: September 2022

With regard to technological innovations, we applied chia (oilseeds) as a stabilizer additive in a normal and waxy potato starch sample to obtain stable starch-based gels during 20 days of storage. The aim of this study was to investigate the 5% w/w normal and waxy potato starch pastes (hot samples) and gels (cold samples) with the addition of 1% w/w whole and ground chia seeds properties as pasting and flow properties of pastes and textural properties of gels. The pasting process using a viscograph showed that normal and waxy potato starch with the addition of chia had a different pasting characteristic. The addition of chia seeds had a greater effect on the properties of normal potato starch than waxy potato starch. From a rheological point of view, starch pastes without chia were less theologically stable as they showed bigger areas of hysteresis loops. Minor changes in the hardness of gels were obtained in normal starch gels with chia seeds during 20 days of storing compared to the samples without chia seeds, whereas in the waxy starch gels, the effect was the opposite.