bluuebunny/crossref_metadata_2025 huggingface dataset:
Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas
Authors: Yang Xinjia , Peng Jialin , Pan Junmin
Published: November 2019

Background

Chlamydomonas reinhardtiiis a unicellular green alga, which is a most commonly used model organism for basic research and biotechnological applications. Generation of transgenic strains, which usually requires selectable markers, is instrumental in such studies/applications. Compared to other organisms, the number of selectable markers is limited in this organism. Nourseothricin (NTC) N-acetyl transferase (NAT) has been reported as a selectable marker in a variety of organisms but not includingC. reinhardtii. Thus, we investigated whetherNATwas useful and effective for selection of transgenic strains inC. reinhardtii. The successful use ofNATwould provide alterative choice for selectable markers in this organism and likely in other microalgae.

Results

C. reinhardtiiwas sensitive to NTC at concentrations as low as 5 µg/ml. There was no cross-resistance to nourseothricin in strains that had been transformed with hygromycin B and/or paromomycin resistance genes. A codon-optimizedNATfromStreptomyces nourseiwas synthesized and assembled into different expression vectors followed by transformation intoChlamydomonas. Around 500 transformants could be obtained by using 50 ng DNA on selection with 10 µg/ml NTC. The transformants exhibited normal growth rate and were stable at least for 10 months on conditions even without selection. We successfully tested thatNATcould be used as a selectable marker for ectopic expression ofIFT54-HA in strains with paromomycin and hygromycin B resistance markers. We further showed that the selection rate forIFT54-HA positive clones was greatly increased by fusingIFT54-HA toNATand processing with the FMDV 2A peptide.

Conclusions

This work represents the first demonstration of stable expression ofNATin the nuclear genome ofC. reinhardtiiand provides evidence thatNATcan be used as an effective selectable marker for transgenic strains. It provides alterative choice for selectable markers inC. reinhardtii.NATis compatible with paromomycin and hygromycin B resistance genes, which allows for multiple selections.
SIRT4 silencing in tumor-associated macrophages promotes HCC development via PPARδ signalling-mediated alternative activation of macrophages
Authors: Li Zhi , Li He , Zhao Zhi-Bo , Zhu Wei , Feng Pan-Pan , Zhu Xi-Wen , Gong Jian-Ping
Published: November 2019

Background

The activation of tumour-associated macrophages (TAMs) contributes to the progression of hepatocellular carcinoma (HCC). SIRT4 acts as a tumour suppressor of tumour growth by regulating cell metabolism, inflammation, and anti-tumourigenesis. However, the involvement of SIRT4 in the activation of TAMs is unknown. Based on previous findings, the expression of SIRT4 in distinct groups of TAMs as well as the effect of SIRT4 silencing on macrophage polarization was investigated.

Methods

The expression of SIRT4 in HCC tissues and peritumour tissues was tested by qRT-PCR, western blotting and histological analysis. A Kaplan-Meier survival curve was generated based on the expression of SIRT4 in the HCC samples. Next, immunofluorescence staining was used to evaluate distinct groups of TAMs in human HCC samples, and the expression of SIRT4 in M1 and M2 TAMs was examined by flow cytometry. A homograft mouse model was used to assess the effect of SIRT4 silencing in TAMs on the development of HCC cells.

Results

SIRT4 was significantly downregulated in HCC tumour tissues, and the expression of SIRT4 in peritumour tissues was positively associated with survival in patients. We further found that downregulation of SIRT4 was associated with increased macrophage infiltration and a high ratio of M2/M1 macrophages in HCC peritumour tissues. Using gene interference, we found that SIRT4 silencing in TAMs significantly modulated the alternative activation of macrophages and promoted in vitro and in vivo HCC cell growth. Mechanistically, we revealed that HCM restricted the expression of SIRT4 in macrophages and promoted alternative activation of macrophages via the FAO-PPARδ-STAT3 axis. Furthermore, we also revealed that elevated MCP-1 expression induced by SIRT4 downregulation was responsible for increased TAM infiltration in peritumour tissues.

Conclusions

Overall, our results demonstrate that downregulation of SIRT4 in TAMs modulates the alternative activation of macrophages and promotes HCC development via the FAO-PPARδ-STAT3 axis. These results could provide a new therapeutic target for the treatment of HCC.
Room4Birth - the effect of an adaptable birthing room on labour and birth outcomes for nulliparous women at term with spontaneous labour start: study protocol for a randomised controlled superiority trial in Sweden
Authors: Berg Marie , Goldkuhl Lisa , Nilsson Christina , Wijk Helle , Gyllensten Hanna , Lindahl Göran , Uvnäs Moberg Kerstin , Begley Cecily
Published: November 2019

Background

An important prerequisite for optimal healthcare is a secure, safe and comfortable environment. There is little research on how the physical design of birthing rooms affects labour, birth, childbirth experiences and birthing costs. This protocol outlines the design of a randomised controlled superiority trial (RCT) measuring and comparing effects and experiences of two types of birthing rooms, conducted in one labour ward in Sweden.

Methods/design

Following ethics approval, a study design was developed and tested for feasibility in a pilot study, which led to some important improvements for conducting the study. The main RCT started January 2019 and includes nulliparous women presenting to the labour ward in active, spontaneous labour and who understand either Swedish, Arabic, Somali or English. Those who consent are randomised on a 1:1 ratio to receive care either in a regular room (control group) or in a newly built birthing room designed with a person-centred approach and physical aspects (such as light, silencer, media installation offering programmed nature scenes with sound, bathtub, birth support tools) that are changeable according to a woman’s wishes (intervention group). The primary efficacy endpoint is a composite score of four outcomes: no use of oxytocin for augmentation of labour; spontaneous vaginal births (i.e. no vaginal instrumental birth or caesarean section); normal postpartum blood loss (i.e. bleeding < 1000 ml); and a positive overall childbirth experience (7–10 on a scale of 1–10). To detect a difference in the composite score of 8% between the groups we need 1274 study participants (power of 80% with significance level 0.05). Secondary outcomes include: the four variables in the primary outcome; other physical outcomes of labour and birth; women’s self-reported experiences (the birthing room, childbirth, fear of childbirth, health-related quality of life); and measurement of costs in relation to the hospital stay for mother and neonate. Additionally, an ethnographic study with participant observations will be conducted in both types of birthing rooms.

Discussion

The findings aim to guide the design of birthing rooms that contribute to optimal quality of hospital-based maternity care.

Trial registration

ClinicalTrials.gov NCT03948815. Registered 13 May 2019—retrospectively registered.
Determinants of late antenatal care follow up among pregnant women in Easter zone Tigray, Northern Ethiopia, 2018: unmatched case–control study
Authors: Weldearegawi Gebrehiwot Gebremariam , Teklehaimanot Berhane Fseha , Gebru Hirut Teame , Gebrezgi Znabu Asfaw , Tekola Kidanemaryam Berhe , Baraki Mulu Ftiwi
Published: November 2019

Objective

The purpose of the study was to identify determinants of late antennal care at first visit in health facilities of eastern zone of Tigray, Northern Ethiopia 2018.

Result

Women with unplanned pregnancy (AOR = 4.03, 95%, CI 1.56–5.67), Participants whose previous first antenatal care was after 16 weeks (AOR = 3.9, 95% CI 1.98–7.68), Participants did not accompanied by their partner for antenatal visit (AOR = 1.29, 95%, CI 1.05–4.67), women recognized their current pregnancy at 3 months or late (AOR = 4.7, 95%, CI 2.49–9.04) and participants provided adequate time for their previous antenatal care by health professionals (AOR = 0.461, 95% CI 0.342–0.875) were found the determinant factors of late antenatal care at first Visit. Hence family planning utilization, times of first visit antenatal, information flow and supporting by partners have a great role in improving antenatal care at first visit. There for responsible bodies should give focuses on utilization of family planning, increasing awareness of pregnancy symptoms and health provisional provide adequate time during visits.
Reconstructive trends and complications following parotidectomy: Incidence and predictors in 11,057 cases
Authors: Bovenzi Cory Donovan , Ciolek Peter , Crippen Meghan , Curry Joseph M. , Krein Howard , Heffelfinger Ryan
Published: November 2019

Background

Parotidectomy is a common treatment option for parotid neoplasms and the complications associated with this procedure can cause significant morbidity. Reconstruction following parotidectomy is utilized to address contour deformity and facial nerve paralysis. This study aims to demonstrate national trends in parotidectomy patients and identify factors associated with adverse postoperative outcomes. This study includes the largest patient database to date in determining epidemiologic trends, reconstructive trends, and prevalence of adverse events following parotidectomy.

Methods

A retrospective review was performed for parotidectomies included in the ACS-NSQIP database between January 2012 and December 2017. CPT codes were used to identify the primary and secondary procedures performed. Univariate and multivariate analysis was utilized to determine associations between pre- and perioperative variables with patient outcomes. Preoperative demographics, surgical indications, and common medical comorbidities were collected. CPT codes were used to identify patients who underwent parotidectomy with or without reconstruction. These pre- and perioperative characteristics were compared with 30-day surgical complications, medical complications, reoperation, and readmission using uni- and multivariate analyses to determine predictors of adverse events.

Results

There were 11,057 patients who underwent parotidectomy. Postoperative complications within 30 days were uncommon (1.7% medical, 3.8% surgical), with the majority of these being surgical site infection (2.7%). Free flap reconstruction, COPD, bleeding disorders, smoking, and presence of malignant tumor were the strongest independent predictors of surgical site infection. Readmission and reoperation were uncommon at an incidence of 2.1% each. The strongest factors predictive of readmission were malignant tumor and corticosteroid usage. The strongest factors predictive of reoperation were free flap reconstruction, malignant tumor, bleeding disorder, and disseminated cancer. Surgical volume/contour reconstruction was relatively uncommon (18%). Facial nerve sacrifice was uncommon (3.7%) and, of these cases, only 25.5% underwent facial nerve reinnervation and 24.0% underwent facial reanimation.

Conclusions

There are overall low rates of complications, readmissions, and reoperations following parotidectomy. However, certain factors are predictive of adverse postoperative events and this data may serve to guide management and counseling of patients undergoing parotidectomy. Concurrent reconstructive procedures are not commonly reported which may be due to underutilization or underreporting.
Discrete representations for Marchenko imaging of imperfectly sampled data
Authors: Wapenaar Kees , van IJsseldijk Johno
Published: November 2019

Marchenko imaging is based on integral representations for focusing functions and Green’s functions. In practice, the integrals are replaced by finite summations. This works well for regularly sampled data, but the quality of the results degrades in a case of imperfect sampling. We have developed discrete representations that account for imperfect sampling of the sources (or, via reciprocity, of the receivers). These representations contain point-spread functions that explain the blurring of the focusing functions and Green’s functions due to imperfect sampling. Deblurring the focusing functions and Green’s functions involves multidimensional deconvolution for the point-spread functions. The discrete representations form the basis for modifying Marchenko imaging to account for imperfectly sampled data, which is important for field data applications.
Thyroid and adrenal function in children born 5 years after the Chernobyl accident in economically unfavourable regions of Belarus
Authors: Boiko Yu. N. , Cherevko A. N. , Petrova A. M. , Zubovich V. K.
Published: November 2019

Currently, there is no doubt the need to study the comprehensive influence of ionizing radiation one the body finctions. Under the influence of ionizing radiation, the development of compensatory and adaptive-adaptive mechanisms of the most radiosensitive organs and tissues to reduce post-radiation effects is of particular importance. Under these conditions, a change in the functional activity of the pituitary-thyroid and adrenal systems is possible, the hormones of which are actively involved in the main adaptation processes. A generally recognized fact is the high radiosensitivity of the above systems, especially in a growing childs body.

In the first days after the accident, the main biologically significant radionuclides were iodine isotopes, which caused the formation of dose loads on the thyroid gland. At present, the long-lived radionuclides 13^Cs and 90Sr have a decisive influence on the radiation situation in the republic. The greatest contribution to the radiation dose is made by cesium radionuclides.

Some authors studied the functional state of the pituitary-thyroid system in children exposed in 1986 to radioactive iodine in the ante- and postnatal periods.

Issues of the occurrence of functional changes in the thyroid gland and adrenal cortex, induced by the intake of radionuclides that are not deposited in these organs, have hardly been studied.

Of considerable interest is the study of the state of the functions of the thyroid gland and adrenal cortex in children born and living under the influence of low doses of radiation, whose mothers were exposed to radioactive iodine.
The sympathoadrenal system in diabetics with different patterns of the disease and its late complications
Authors: Chanclrashekar-Reddy K. M. , Balabolkin M. I. , Stoilov L. D.
Published: November 2019

Blood plasma concentrations of noradrenaline, dopamine, serotonin and their metabolites (DOPAC, HVA) were measured in 28 patients with insulin-dependent and 32 with noninsulin-dependent diabetes mellitus (IDDM and NIDDM, respectively). The patients were divided into 4 groups. Group 1 were 15 patients without late diabetic complications, group 2 were 15 subjects with diabetic neuropathy, group 3 were patients with neuropathy and retinopathy (n=16), and group 4 were 14 patients with neuropathy, retinopathy, and nephropathy. The results showed an increase of serotonin levels in IDDM patients w. those with NIDDM, a positive correlation between serotonin and blood glucose levels in IDDM, increased concentration of dopamine and reduced plasma level of noradrenaline in patients with diabetic neuropathy vs. those without late diabetic complications. Plasma levels of dopamine were decreased in all the patients microvascular involvement. The findings indicate the development of changes in the sympathoadrenal system of patients with late diabetic vascular complications.
SOFRIMENTO PATOGÊNICO DE AGENTES COMUNITÁRIOS DE SAÚDE EM UMA UBSF
Authors: Rodrigues Maria Luiza Moraes , Lima Samuel Carmo
Published: November 2019

O presente estudo busca compreender como o desenvolvimento do sofrimento patogênico de ACS se relaciona a organização do trabalho na Estratégia Saúde da Família em decorrência disto os objetivos específicos são: caracterizar o perfil sociodemográfico dos ACS participantes do estudo; analisar a organização do processo de trabalho sob o olhar do ACS e investigar as possíveis situações geradoras de sofrimento no trabalho do ACS. Para tanto se propõe uma abordagem qualitativa, conduzida por meio da observação participante e entrevistas individuais semiestruturadas com agentes comunitários da Estratégia Saúde da Família do Município de Uberlândia - MG. O material selecionado foi submetido à Análise de Conteúdo Temático e interpretado segundo o referencial teórico de Christophe Dejours sobre o sofrimento patogênico, a partir das seguintes categorias temáticas: a) Cotidiano de Trabalho dos ACS; b) Fontes de Prazer no Trabalho, c) Frustrações diante da incapacidade de resolver os problemas; d) Questões que interferem no processo de trabalho. O perfil sócio demográfico na pesquisa evidenciou um predomínio de ACS do sexo feminino, casadas, com ensino superior incompleto, adultos jovens e tempo de atuação entre 25 a 36 meses. A análise do cotidiano de trabalho dos ACS destacou que a principal atividade realizada dentre as que executa é a Visita Domiciliar e que outras determinadas pelo serviço divergem das prescritas pelo Ministério da Saúde. Ficou demonstrado que a satisfação profissional está intrinsicamente ligada a resolutividade de suas ações; a impotência e a incapacidade decorrente da ausência de suporte ou inexistência de ferramentas para atender as demandas da comunidade podem gerar no ACS o sofrimento. Na análise das questões que interferem no processo de trabalho dos ACS e que provocam angústia e insatisfação ficou demonstrado que a culpabilização de um agente externo pela não realização efetiva de seu trabalho é utilizada como estratégia de defesa. Apesar disso, os ACS estão afetivamente ligados ao seu trabalho, o que o torna fonte de sublimação e promotora de saúde. Diante disso propõe-se a reavaliação da organização do processo de trabalho.
PADRÕES ESPACIAIS DA OCORRÊNCIA DE LEISHMANIOSE VISCERAL HUMANA NA CIDADE DE NATAL-RN: A INFLUÊNCIA DAS ÁREAS DE RISCO SOCIAL
Authors: Teixeira Karen Kaline Teixeira , Nascimento Kennedy Gomes do , Santana Reginaldo Lopes , Souza Ana Mayara Gomes de , Souza Talita Araujo de , Barbosa Isabelle Ribeiro
Published: November 2019

Objetivou-se nesse estudo analisar a ocorrência de casos de Leishmaniose visceral humana (LVH) e sua proximidade com as áreas de risco social no município de Natal-RN. Foram analisados 285 casos confirmados de LVH registrados no SINAN no período de 2008 a 2018. Para a sobreposição dos casos e das áreas de Risco social utilizou-se a análise de Kernel. Foi calculada a distância média anual de cada ponto à área de risco mais próxima. A densidade de Kernel revelou que a ocorrência dos casos é mais predominante nas áreas periféricas, sobretudo em áreas localizadas na região Norte e Oeste e sobrepostas às áreas de risco social. A distância média entre os casos e a área de risco mais próxima foi de 445 metros. Observou-se uma redução da distância média entre os casos e as áreas de risco social a cada ano, no período de 2008 e 2013, e um aumento no período de 2014 a 2018. A menor distância média foi registrada em 2012 (227 metros) e a maior foi em 2017 (677 metros). A modelagem espacial apontou que a dinâmica da ocorrência da leishmaniose visceral no município de Natal-RN está relacionada à proximidade das áreas de risco social.
MONITORAMENTO DE AEDES AEGYPTI POR OVITRAMPAS E PELO MÉTODO LIRAa EM SALGUEIRO, PERNAMBUCO, BRASIL
Authors: Gonçalves e Sá Ádilla Katarinne , Matias Gomes Erasmo José , Beltrão Rameh Barbosa Ioná Maria , Medeiros de Araújo Frutuoso Maria Núbia , Costa Castro Lyra Marília Regina , Mansur Custódio Nogueira Rejane Jurema , Ferreira Brandão Rodrigues Sofia Suely
Published: November 2019

A pesquisa objetiva avaliar a infestação de Aedes aegypti no bairro Santa Margarida localizado no município de Salgueiro, Pernambuco, utilizando ovitrampas e o método simplificado de amostragem do índice de infestação por Ae. aegypti. Foram instaladas 10 ovitrampas, em diferentes quarteirões do bairro, monitoradas entre outubro de 2017 e 2018, com palhetas coletadas a cada 15 dias, para contagem dos ovos. O índice de infestação nos quarteirões foi comparado pelo teste de Scott-Knott, o de positividade das ovitrampas (IPO) e o de densidade de ovos (IDO) pela correlação de Pearson. Com o teste de Mann-Whitney obteve o número médio de ovos. Analisou-se 248 palhetas e coletou 4.770 ovos. O IPO foi de 34,7% e o IDO de 19,2 ovos, cuja correlação por ciclo foi significativo (p=0,82). A análise de variância (ANOVA) indicou níveis de infestação diferentes entre os quarteirões (p=6,41E-06). A baixa precipitação (u=148,5, p=0,1342 e Z-escore=-1,56866) e alta umidade (u=66,5, p=0,05614 e Z-escore=1,9061) contribuíram para a proliferação do mosquito, evidenciadas nos meses de março e junho de 2018, período em que aumentou o número de ovos e de larvas. Os resultados mostraram a importância da utilização conjunta de ovitrampas e do Levantamento Rápido de Índices para Aedes aegypti (LIRAa), potencializando a segurança e a eficácia do monitoramento do mosquito.
Perancangan dan Penataan Kawasan di Sepanjang Sungai Banjir Kanal Timur Kelurahan Bugangan dan Kelurahan Rejosari dengan Konsep Livable Riverfront Settlement Kecamatan Semarang TImur, Kota Semarang
Authors: Puteri R.D.A. , Pulungan R.S. , Tambunan H.D. , Rahmi H.M. , Amalia R. , Davinaldo A.R. , Hidayat M.W. , Mazz M. , Febriani D.D. , Zulhamdi Z. , Sima H.
Published: November 2019

Livable Riverfront Settlement is a concept design that is mindful of environmental livability housing which is in the nearby river. The concept of Liveable Riverfront Settlement is a derivative of the concept of macro-regions planning Sustainable Riverfront Settlement and the concept of the planning area of meso Ecological Riverfront Settlement. The purpose of this writing is focused on the design of landing neighborhood along the Eastern Canal flood in Bugangan and Rejosari village who have a problem like slums and squatters settlement, the occurrence of floods, the lack of open space, the low quality of the environment and housing backlog.In this article using quantitative methods with descriptive analysis techniques, and the qualitative method with interviews and observations of the field. The results of the identification and analysis of the design of landing (UDGL) settlement along the Eastern Canal flood in Bugangan and Rejosari village based on 3 indicators, the improvement of the economy through the provision of employment (job creation), the management of flood risks through the improvement of environmental quality (disaster risk management), as well as the provision of housing that is affordable and livable (livable housing provision).
Persepsi Pengunjung Terhadap Tingkat Kenyamanan Alun-Alun Kabupaten Pemalang
Authors: Paningkat Galang Kahar , Khadiyanto Parfi
Published: November 2019

Population growth rates and city development which is increasing drastically will increasingly improve urban services.Same time it will have a negative impact on the protection of nature so that in realizing a sustainable city, it is necessary to provide green open space as a counterweight to the environment. The form of green open space in urban areas is a parks. Pemalang Regency has a city park that is Pemalang Town Square. Public space meetings are a basic requirement. If there is no comfort in public spaces, it is difficult to see the needs of visitors in public spaces as users. the purpose of the study was to study the comfort level of Pemalang Town Square according to the perceptions of visitors. Visitors are taken as research objects because they are users of green open space, so their position is important as an evaluation of a planning program.The results of the study are the comfort level of Pemalang Town square based on the perceptions of visitors included in the convenient category. However, there are still aspects that still need to be improved to improve comfort such as lighting facilities in the park that are dim at night.
Multimodal feature learning and fusion on B-mode ultrasonography and sonoelastography using point-wise gated deep networks for prostate cancer diagnosis
Authors: Zhang Qi , Xiong Jingyu , Cai Yehua , Shi Jun , Xu Shugong , Zhang Bo
Published: November 2019

B-mode ultrasonography and sonoelastography are used in the clinical diagnosis of prostate cancer (PCa). A combination of the two ultrasound (US) modalities using computer aid may be helpful for improving the diagnostic performance. A technique for computer-aided diagnosis (CAD) of PCa is presented based on multimodal US. Firstly, quantitative features are extracted from both B-mode US images and sonoelastograms, including intensity statistics, regional percentile features, gray-level co-occurrence matrix (GLCM) texture features and binary texture features. Secondly, a deep network named PGBM-RBM2 is proposed to learn and fuse multimodal features, which is composed of the point-wise gated Boltzmann machine (PGBM) and two layers of the restricted Boltzmann machines (RBMs). Finally, the support vector machine (SVM) is used for prostatic disease classification. Experimental evaluation was conducted on 313 multimodal US images of the prostate from 103 patients with prostatic diseases (47 malignant and 56 benign). Under five-fold cross-validation, the classification sensitivity, specificity, accuracy, Youden’s index and area under the receiver operating characteristic (ROC) curve with the PGBM-RBM2 were 87.0%, 88.8%, 87.9%, 75.8% and 0.851, respectively. The results demonstrate that multimodal feature learning and fusion using the PGBM-RBM2 can assist in the diagnosis of PCa. This deep network is expected to be useful in the clinical diagnosis of PCa.
Multidisciplinary team management of carcinoid heart disease
Authors: Steeds Richard P , Sagar Vandana , Shetty Shishir , Oelofse Tessa , Singh Harjot , Ahmad Raheel , Bradley Elizabeth , Moore Rachel , Vickrage Suzanne , Smith Stacey , Yim Ivan , Elhassan Yasir S , Venkataraman Hema , Ayuk John , Rooney Stephen , Shah Tahir
Published: November 2019

Carcinoid heart disease (CHD) is a consequence of valvular fibrosis triggered by vasoactive substances released from neuroendocrine tumours, classically in those with metastatic disease and resulting in tricuspid and pulmonary valve failure. CHD affects one in five patients who have carcinoid syndrome (CS). Valve leaflets become thickened, retracted and immobile, resulting most often in regurgitation that causes right ventricular dilatation and ultimately, right heart failure. The development of CHD heralds a significantly worse prognosis than those patients with CS who do not develop valvular disease. Diagnosis requires a low threshold of suspicion in all patients with CS, since symptoms occur late in the disease process and clinical signs are difficult to elicit. As a result, routine screening is recommended using the biomarker, N-terminal pro-natriuretic peptide, and regular echocardiography is then required for diagnosis and follow-up. There is no direct medical therapy for CHD, but the focus of non-surgical care is to control CS symptoms, reduce tumour load and decrease hormone levels. Valve surgery improves long-term outcome for those with severe disease compared to medical management, although peri-operative mortality remains at between 10 and 20% in experienced centres. Therefore, care needs to be multidisciplinary at all stages, with clear discussion with the patient and between teams to ensure optimum outcome for these often-complex patients.
Source Analysis as a Necessary Condition for Preparing an Edition’s Text (on the Material of the New Testament Prepared by the Editorial Circle of Epiphany Slavinetsky and Evfimi Chudovsky, Second Half of the 17th Century)
Authors: Pentkovskaya Tatiana V.
Published: November 2019

The Church Slavonic New Testament of the second half of the 17th century, translated under the leadership of Epiphanius Slavinetsky and Euthymius of Chudov Monastery, belongs to the translations made from several originals in different languages. The main source of the translation includes additional texts derived mainly from Polish sources. Their study is important for the reconstruction of the general idea of the translators and of the type of edition prepared by them.
Quality of life in the big city (the case Dnipro city)
Authors: Бойко В. А.
Published: November 2019

The article discusses the heuristic possibilities of objective and subjective approaches to determiningthe quality of life of individuals in modern society. In order to construct a measurement of the quality oflife of the population, objective and subjective indicators were defined in this perspective: quantitativeones that record the presence or absence of a certain property and qualitative ones that record the extentof its manifestation and development. For the empirical representation of the quality of life of residentsof the city of Dnipro, data from sociological studies, the «Dnipro – my hometown» and the fourth all-Ukrainian municipal IRI survey were used.Possibilities and conditions for self-realization of the person are characterized, subjective level ofevaluation of strategies of success, professional prospects, achievement of material well-being in theurban space is determined through the prism of age differentiation. Positive shifts in the perception of thecity as an inclusive space (the spread of inclusive education, the opening of children’s and playgrounds,which take into account the needs of children with disabilities , etc.) have been noticed.Characteristics of urban social life were determined through fragmentation, disunity, decentralization,dehumanization. They are becoming the markers of modern society. These processes affect the emotionalinternal state of the individual and determine the sphere of his social fears. The main factors that causeanxiety and fear in the mass consciousness of the residents of the city are related to economic issues –rising prices, loss of work, closure of enterprises. The structure of expenditures and the assessment ofwell-being, as well as the level of satisfaction with material goods, have been determined. The subjectivecharacteristic of the labor market and opportunities to engage in entrepreneurial activity are provided. Thegeneral summary of the quality of life in Dnipro is presented in the Summary of Capability AssessmentIndex.
Educar en las tic´s a niños en situación de pobreza
Authors: García Cuarte Juan Carlos
Published: November 2019

El propósito fundamental de esta reflexión se orienta a construir espacios estratégicos y pedagógicos que favorezcan la utilización de las Tecnologías de la Información y la Comunicación (TICS) en la población infantil en situación de pobreza en San José de Cúcuta, Colombia. Esta población está constituida por estudiantes, padres de familia, docentes y directivos de las instituciones de educación básica del Municipio y su fin es optimizar las prácticas pedagógicas de los profesores y, por ende, el aprendizaje y logro de competencias en un área en desarrollo, como es la informática. El escenario que servirá de referencia contextual para desarrollar la reflexión es el Cadel 3 del Municipio de Cúcuta, Departamento Norte de Santander
Piloting an Extracurricular Quality Improvement and Patient Safety Training Program for Interprofessional Learners
Authors: Rajaram Akshay , Lee-Daigle Mialynn , Eade Malcolm , Curry Anna , Connelly Robert , Ilan Roy
Published: November 2019

This article was migrated. The article was not marked as recommended. Introduction Experts recommend that health professions students acquire knowledge and skills in quality improvement and patient safety. Educational initiatives exist, but involve minimal interprofessional contact and experiential learning. We piloted an extracurricular program combining didactic elements and projects to address these issues. Methods We collected demographic information and administered a post-program survey to assess the pilot's reach and impact. We analyzed responses using simple descriptive statistics and thematically analyzed unstructured feedback. Results Fifty-one students participated, including twenty-one (41%) undergraduate students, sixteen (31%) graduate students, and fourteen (27%) medical students. Nineteen (37%) participants responded to the survey. Qualitatively, themes around workshop effectiveness, program administration, project-student mismatch, and engagement and accountability emerged. Discussion Despite limited response rates, our training program appeared to be well received. However, key issues of engagement and impact remain. Future efforts will focus on improvement in these areas and more rigorous evaluation of learning outcomes.
Does antenatal education decrease the cesarean rates in nulliparous women?
Authors: TÜRKLER Can
Published: November 2019

Objective: In recent years, cesarean rates have increased
and healthcare systems have tried several ways to correct this situation.
Antenatal education programs are one of them. The aim of this study was to determine the effect of
antenatal education on cesarean rates in nulliparous women.Material and
Methods: This is a retrospective, cohort study. In total,
200 nulliparous women who were given birth at our hospital (educated group,
n = 86 and uneducated group, n = 114) participated in the study. Data were collected from the antenatal
education class forms and patient files.Results: The rate of cesarean section in the group receiving antenatal education was found 30.2% and the rate of cesarean section in the uneducated group was 36.8%. It was statistically significant (p<0,05).

Conclusion: The rate of
cesarean section decreases in nulliparous women who were taken antenatal
education. In addition, antenatal education programs must be standardized and
the number of participants should be increased all over the world.
AAV Titration by qPCR Using SYBR Green Technology v1
Authors: The Nonprofit Plasmid Repository Addgene
Published: November 2019

This protocol goes through AAV titration by qPCR using SYBR Green Technology. To see the full abstract and additional resources, visit the Addgene protocol page. Sample Data References Aurnhammer C, Haase M, Muether N, Hausl M, Rauschhuber C, Huber I, Nitschko H, Busch U, Sing A, Ehrhardt A, Baiker A. Universal real-time PCR for the detection and quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28.PMID: 22428977
Seeding v2
Authors: Javier Quero Francisco , L´Homme Iriarte Alfredo , Armero Laura
Published: November 2019

This protocol describes the procedures to seed the Bacullis subtilis inside the microfluidic chip to create the biofilms.
Prevalence of risk factors of cardiovascular complications in elderly women with left breast cancer
Authors: Vologdina I. V. , Poroshina E. G. , Zhabina R. M. , Stanzhevsky A. A.
Published: November 2019

The purpose to study the risk factors for elderly women with left breast cancer at the stage of chemotherapy and radiotherapy in the context of daily clinical practice for early detection of possible cardiovascular complications and optimization of therapy.

Material and methods. 69 women with HER2 neu negative left breast cancer without severe cardiovascular disease were examined. The first group included 39 elderly patients (67.7 3.8 years). The second group consisted of 34 middle-aged patients (49.8 5.7 years). In addition to the risk assessment according to the Score scale, additional factors including psychosocial factors were studied. The examination was carried out before the start of treatment, after the end of the course of doxorubicin in a cumulative dose of up to 360 mg/m2 and after the completion of radiation therapy 3D conformal radiation therapy SOD 39 Gr.

Results. In elderly patients, risk factors such as obesity, increased cholesterol, and hypertension were significantly more common. In both study groups, low physical activity was revealed associated with both cancer itself and the treatment. All examined patients had moderate reactive anxiety on the Spielberger-Hanin scale. Elderly patients showed high level of personal anxiety; the number of points scored 49.3 3.6. According to the Score scale, a moderate risk was detected in 18 (58.1%) and a high risk in 13(41.9%) elderly patients. After doxorubicin treatment asymptomatic systolic dysfunction was detected in 8 (20.5%) patients (decrease in EF50%), 21 (75%) diastolic dysfunction with relaxation slowdown (E/A1). In 9 (23.1%) of the women without reducing the EF fibrotic changes in the myocardium of the left ventricle was revealed. In 8 (20.5%) women a thickening of the pericardial layer was revealed.

Conclusion. The findings suggest the need for a personalized approach and assessment of risk factors in patients of different age groups with left breast cancer at the stage of preparation and conduct of chemoradiotherapy. This highlights the need for enhanced history collection and consideration of not only key but also additional risk factors. The results of the study can be used in the work of practical health care institutions for the planning, development, implementation and control of chemoradiography safety in terms of preventing cardiovascular complications.
Directivity of M 3.1 Earthquake near Anza, California and the Effect on Peak Ground Motion
Authors: Fletcher Jon B. , Boatwright John
Published: November 2019

We show the effect of rupture directivity on peak ground-motion values for a moderate magnitude event at Anza, California, and neighboring stations at the Imperial Valley. The event was located near Borrego Springs on the west side of the Salton Sea and was well recorded at broadband stations near Anza, California, and at stations on the west side of the Imperial Valley. After correcting for regional attenuation, an anomalously large residual in peak motion was observed at station ERR just to the southeast of the epicenter. Using the algorithm from Boatwright (2007), peak motions from the regional seismic networks in southern California were inverted to determine directivity, which was to the southeast along the trend of the San Jacinto fault toward station ERR. This algorithm uses peak values compiled for the ShakeMap system mostly at regional distances. It does not capture the main features of the source time function (STF) predicted by directivity. Consequently, we determined the second-degree moments for this earthquake, which confirmed that station ERR has a shorter and higher STF compared to stations to the northwest suggesting rupture propagated to the southeast. The azimuthal distribution of local stations is sparse, but nevertheless the largest amplitudes (such as at station ERR) correlate well with the maximum in the radiation pattern and smaller values with the minima, which is the radiation pattern for SH plus the effect of directivity. Using the data from the analysis of the second-degree moments, the characteristic length of the fault is 0.58 km, assuming an idealized unilateral extended rupture with a rupture time of 0.09 s. This yields an apparent rupture velocity of 6.4  km/s for an idealized model, which is super shear. This value is model dependent and would change if, for example, the rupture was bilateral. Although this value is even greater than the P-wave velocity, it supports the idea that the rupture velocity is super shear and would enhance the correlation between the peak motions and the radiation pattern.
Time deals: optimization of lag
Authors: Shabanov Timofey Yu.
Published: November 2019

Relevance. In many ways, a successful purchase and sale transaction is determined by the time it takes to complete it. The search for the best deal time is linked to the optimization of the time lag. The purpose of the study is to optimize the time lag of the agricultural market.

Methods. This article considered a role of time in business processes for Russian potato market of producer into period 1998-2018.

Results. There was trend of annual reduction about 365 thousand tons (1.2...1.7% of total) per year supply of potatoes on Russian domestic market of producers. Some priority of quantity vs price of producer been revealed. There was inverse proportional function between producers` prices and quantity of potatoes. That is than worse harvest that higher producer`s price. Reducing a supply of potatoes 1 million tons from agricultural market perhaps increase in producer prices by $ 8.5 / t (4...5% on average). Offered concept and hypothesis about linearization of equilibrium of market processes into conditions of time shifts of dynamics their parameters were confirmed. There verified assumption about existence of inter-interval time optimum which this linearization maximal business processes. In course of correlation analysis of dynamics of annual indicators of potato market, it revealed month May of current period as optimal time of producer's offers with price restrictions and diminishing returns, and month September with quantity restrictions and increasing returns. If fixation of producer`s prices situation of deals May more likely, in case of crop failure (limited quantity) in September.
Gensini score predicts postoperative atrial fibrillation following on-pump coronary artery bypass grafting
Authors: KARAÇAĞLAR Emir , AKGÜN Arzu , ÇİFTCİ Orçun , MÜDERRİSOĞLU Haldun
Published: November 2019

Aim:  Postoperative
atrial fibrillation (POAF) is common after coronary artery by-pass grafting
surgery (CABG) and associated with adverse events. GENSINI score is a
widely-used scoring system that quantifies angiographic atherosclerosis. The
value of GENSINI score to predict POAF has not been studied previously. Aim of
this study was to evaluate this relationship in our patients undergoing on-pump CABG surgery.Material and Methods: In this
retrospective, single-center study, a total of 113 patients without exclusion criteria were included.
Demographic and clinical characteristics of patients were recorded and GENSINI scores
were calculated. Univariate
and multivariate logistic regression analyses were performed to identify the
independent predictors of POAF.Results: Postoperative atrial fibrillation developed in
34 (30%) patients. Multivariate logistic regression analyses
revealed that C-Reactive Protein [ß=0.037, p=0.011, OR=1.03, 95% CI
(1.008-1.065)] and GENSINI score [ß=0.015, p=0.024,OR=1.012, 95% CI
(1.002-1.028)]  are independent
predictors of POAF. The ROC analysis showed an area under the curve of 0.587 (p=0.012).  GENSINI score of > 50.75 had 82.4%
sensitivity in predicting POAF [p=0.012, 95%CI (0.464-0.711)].

Conclusion: Our results showed
that GENSINI score is an independent predictor of POAF in patients undergoing on-pump CABG surgery.
Editöre mektup: Vücut kitle indeksi ile ortalama trombosit hacmi arasında herhangi bir ilişki bulunmayabilir
Authors: BEYAN Cengiz , BEYAN Esin
Published: November 2019

Karakurt ve arkadaşlarının çocuk ve adölesanlarda vücut kitle indeksi ile ortalama
trombosit hacmi (MPV) arasındaki ilişkiyi inceleyen araştırmalarını büyük bir ilgi
ile okuduk Biz bu çalışma hakkında yorum
yapmak istiyoruz.
Ozon uygulaması ve oral cerrahideki yeri: Derleme
Authors: BOĞAÇ Pembe , ERKMEN Erkan
Published: November 2019

Ozon
(O₃) üç oksijen atomundan oluşan  renksiz
, keskin kokulu doğal bir gazdır. Atmosferin stratosfer tabakasında bulunur ve
güneşten gelen zararlı ultraviyole ışınlarını süzer. Keşfinden sonraki ilk
yıllarda dezenfeksiyon amacıyla kullanılırken yıllar içinde yapılan çalışmalar
tıp alanında kullanımını artırmıştır. Antimikrobiyal , biyosentetik, analjezik
, antihipoksik , immünmodülatör gibi özellikleri bulunan ozonun tedavi amacıyla
kullanılması ile ilgili günümüzde  birçok
çalışma yapılmaktadır. Bu derlemede ozon ve ozonun  kullanım alanları ile yara iyileşmesi
üzerindeki etkilerinden bahsedilmiştir.
Successful cryoablation of junctional tachycardia in an elderly patient: A case report
Authors: KORKMAZ Ahmet , ÇELİK Muhammet Cihat , GÜRSOY Havva Tuğba , BAŞYİĞİT Funda , GÜRAY Ümit
Published: November 2019

Junctional tachycardias(JT) are rare supraventricular
tachyarrhythmias observed mainly in infants and children. JT is rare in adult
patients, and it is associated with poor outcomes. In general, these supraventricular tachycardias
respond insufficiently to pharmacological treatment. In this report, we present the case of a 72-year-old patient with
incessant tachyarrhythmia in whom cryoablation of the para-Hisian ectopic focus
was successfully performed.
Adölesan idiopatik skolyozlu hastalarda Boston tipi korse kullanımının yaşam kalitesine etkisi
Authors: YAPAR Aliekber , ŞENKÖYLÜ Alpaslan
Published: November 2019

Amaç: AdölesanİdiopatikSkolyoz
(AİS) nedeniyle konservatif tedaviuygulanan hastalarda, korse (Boston tipi)  kullanımının yaşam kalitesine etkisiniSkolyoz
Araştırma Cemiyeti’nin Sağlık ilişkili Yaşam Kalitesi-22 (Scoliosis Research
Society- 22; SRS-22) anketi ile değerlendirmekGereç ve Yöntemler: Araştırma
kesitsel tipte bir çalışmadır. Gazi Üniversitesi Tıp Fakültesi Hastanesi
Ortopedi ve Travmatoloji Kliniğinde 2008 - 2017 yılları arasında tanı almış ve
en az bir yıldır AİS tanısıyla takip edilen, konservatif tedavi uygulanan, yaş
ortalaması 16,0±2,9 (min:11,00; maks:26) olan 22’si erkek (%32,4), 46’sı kadın
68hasta çalışmaya dâhil edilmiştir. Hastaların yaşam kalitesini değerlendirmek
için SRS-22 anketi kullanılmıştır.Bulgular:
Çalışmaya katılan hastaların23’ü (%33,8)korse ile45’iise korsesiz takip
edilmiştir.Hastaların son kontroldeki Cobb açısı dereceleri korse ile takip
edilen grupta istatistiksel olarak anlamlı yüksek bulunmuştur (p<0,001).
Korse ile takip edilen ve korsesiz takip edilen her iki grup arasında SRS-22
toplam skoru, ağrı, fonksiyon, ruh sağlığı ve tedaviden tatmin skorları
açısından istatistiksel olarak anlamlı fark bulunmamıştır (p>0,05). SRS-22
görünüş skorunun ise korse ile takip edilen grupta istatistiksel olarak anlamlı
düzeyde daha düşük olduğu saptanmıştır (p=0,019).

Sonuç:
Bu çalışmada korseli hastaların yaşam kalitelerininkorsesiz takipedilenlerle
benzer olduğu saptanmıştır. Korseli grubun Cobb açı değerlerinin diğer gruba
göre daha yüksek olması nedeniyle SRS-22 görünüş skorunun korselilerde daha
düşük olduğu saptanmıştır. Korseli hastaların ağrı, fonksiyon, ruh sağlığı ve
tedaviden tatmin ile ilişkili yaşam kalitelerinin benzer olduğu ve genel olarak
yaşam kalitelerinin korseden etkilenmediği bulunmuştur.
Múltiples caras de la inmigración cubana. Tesis para un debate
Authors: Cobas Cobiella María Elena
Published: November 2019

El trabajo tiene como objetivo introducir al lector en algunas cuestiones de interés en relación a la normativa migratoria cubana, resaltando algunos conceptos de relevancia para los lectores ávidos en la materia y sobre todo en el complejo mundo del derecho cubano sumergido en las indefiniciones.  Trata además de la cuestión de la “llamada repatriación “como punto central del debate.