bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Determination of Antimicrobial, Antioxidant and Antiviral Properties of Greek Plant Extracts
Authors: Tsakni Aliki , Kyriakopoulou Eirini , Letsiou Sophia , Halvatsiotis Panagiotis , Rigopoulos Haralambos , Vassilaki Niki , Houhoula Dimitra
Published: January 2025

The medicinal potential of plant extracts, especially their antimicrobial, antioxidant, antiviral and cytotoxic properties, has gained significant attention in recent years. This study examined the in vitro bioactivities of several selected Greek medicinal plants, like Eucalyptus globulus L., Thymus vulgaris L., Salvia rosmarinus L. and Ocimum basilicum L., are well-known for their traditional therapeutic use. Minimum inhibitory concentration (MIC) assays were used to evaluate the antimicrobial activity of the extracts against pathogenic bacteria. The antioxidant activity was carried out using the DPPH method, while the cytotoxicity of the plants was determined using the Alamar Blue method. In addition, the antiviral efficacy of the samples was tested against DENV in different cell lines. The majority of medicinal herbs demonstrated significant antimicrobial action (MIC = 30–3000 μg∙mL−1). The extracts showed great antioxidant activity, while the Salvia rosmarinus L. extract turned out to be the most effective (IC50 = 12.89 ± 0.11 μg∙mL−1). In contrast, the extract of Eucalyptus globulus L. had the lowest antioxidant action (IC50 = 71.02 ± 0.42 μg∙mL−1). The results of the Alamar Blue method were presented with CC50 values, and it was shown that Eucalyptus globulus L. extract exhibited the highest cytotoxicity (CC50 = 5.94% v/v ± 0.04). Similarly, the results of the antiviral potential of extracts were expressed as EC50 values, and Eucalyptus globulus L. was characterized as the most effective sample against dengue virus infection, with EC50 values estimated at 2.37% v/v ± 0.6 (HuhD-2 cells infected with DENV-2) and 0.36% v/v ± 0.004 (Huh7.5 cells infected with DVR2A). These findings provide a foundation for further studies in order to combat infectious diseases and promote human health.
Research Progress on the Structure and Function, Immune Escape Mechanism, Antiviral Drug Development Methods, and Clinical Use of SARS-CoV-2 Mpro
Authors: Ren Jiayi , Zhang Zhengfu , Xia Yi , Zhao Daqun , Li Dingqin , Zhang Shujun
Published: January 2025

The three-year COVID-19 pandemic ‘has’ caused a wide range of medical, social, political, and financial implications. Since the end of 2020, various mutations and variations in SARS-CoV-2 strains, along with the immune escape phenomenon, have emerged. There is an urgent need to identify a relatively stable target for the development of universal vaccines and drugs that can effectively combat both SARS-CoV-2 strains and their mutants. Currently, the main focus in treating SARS-CoV-2 lies in disrupting the virus’s life cycle. The main protease (Mpro) is closely associated with virus replication and maturation and plays a crucial role in the early stages of infection. Consequently, it has become an important target for the development of SARS-CoV-2-specific drugs. This review summarizes the recent research progress on the novel coronavirus’s main proteases, including the pivotal role of Mpro in the virus’s life cycle, the structure and catalytic mechanism of Mpro, the self-maturation mechanism of Mpro, the role of Mpro in virus immune escape, the current methods of developing antiviral drugs targeting Mpro, and the key drugs that have successfully entered clinical trials. The aim is to provide researchers involved in the development of antiviral drugs targeting Mpro with systematic and comprehensive information.
Novel Approaches to Monitor Pharmacokinetics and Metabolism of Gemcitabine-Ibandronate Conjugate in Mice and Dogs
Authors: Klawitter Jost , Easton Mckay , Karpeisky Alexander , Farrell Kristen B. , Thamm Douglas H. , Shokati Touraj , Christians Uwe , Zinnen Shawn Patrick
Published: January 2025

Background. The use of the bone-seeking properties of bisphosphonates (BPs) to target the delivery of therapeutic drugs is a promising approach for the treatment of bone metastases. Currently, the most advanced example of this approach is a gemcitabine-ibandronate conjugate (GEM-IB), where the bone-targeting BP ibandronate (IB) is covalently linked to the antineoplastic agent gemcitabine (GEM) via a spacer phosphate group. In the present study, we describe the development of a new analytical platform to evaluate the metabolism and pharmacokinetics of GEM-IB in mice and dogs and the results of proof-of-concept studies assessing the pharmacokinetics of GEM-IB in dogs and mice. Methods. We validated analytical platforms to analyze GEM-IB and five of its major metabolites IB, gemcitabine-5′-phosphate (GEMMP), gemcitabine (GEM), 2′,2′-difluoro-2′-deoxyuridine-5′-phosphate (dFdUMP), and 2′,2′-difluoro-2′-deoxyuridine (dFdU) and performed proof-of-concept pharmacokinetic studies in mice (5 mg/kg i.p.) and dogs (5 mg/kg i.v.). Results. Intra- and inter-run accuracy and imprecision (3 days) of the assays met the (FDA) acceptance criteria. The proof-of-concept plasma pharmacokinetic studies in mice showed AUCs of 1278, 10,652, 405, 38, 1063, 3389, and 38 h·ng/mL for GEM-IB, IB, GEMMP, dFdU-MP, GEM, and dFdU, respectively. In dog plasma, AUCs of 295, 5725, 83, 11, 1625, and 6569 h·ng/mL were observed for GEM-IB, IB, GEMMP, dFdUMP, GEM, and dFdU. Conclusions. Pharmacokinetic studies in dogs and mice showed that GEM-IB is rapidly converted to IB and GEM; dFdU is formed (from GEM) with a delay. The rapid disappearance of GEM-IB from circulation could be explained by a combination of metabolism and rapid distribution to tissue/bone.
Managing ADHD Symptoms in Children Through the Use of Various Technology-Driven Serious Games: A Systematic Review
Authors: Doulou Aikaterini , Pergantis Pantelis , Drigas Athanasios , Skianis Charalampos
Published: January 2025

Children with attention deficit hyperactivity disorder (ADHD) frequently experience impairments in a range of abilities. Due to their poor attention and concentration, they find it challenging to stay focused when learning. They need help to retain the directions given by teachers and are very animated. Focus issues, hyperactivity, and attention problems may hamper learning. The needs and challenges of children with ADHD have been addressed by numerous digital solutions over the years. These solutions support a variety of needs (e.g., diagnosing versus treating), aim to address a variety of goals (e.g., addressing inattention, impulsivity, working memory, executive functions, emotion regulation), and employ a wide range of technologies, including video games, PC, mobile, web, AR, VR, tangible interfaces, wearables, robots, and BCI/neurofeedback, occasionally even in tandem. According to studies on the psychological impacts of serious games, immersive games can potentially be valuable tools for treating ADHD. This research investigates using PC, mobile/tablet applications, augmented reality, virtual reality, and brain–computer interfaces to develop executive functions and metacognitive and emotional competencies in children with ADHD through serious games. Following PRISMA 2020 criteria, this systematic review includes a comprehensive search of the PubMed, Web of Science, Scopus, and Google Scholar databases. The database search provided 784 records, and 30 studies met the inclusion criteria. The results showed that serious games assisted by multiple technologies could significantly improve a wide range of cognitive and socioemotional meta-competencies among children with ADHD, including visuospatial working memory, attention, inhibition control, cognitive flexibility, planning/organizing, problem-solving, social communication, and emotional regulation. The results of this review may provide positive feedback for creating more inclusive digital training environments for the treatment of ADHD children.
Prevalence of Leptospira spp. in Lithuanian Wild Boars (Sus scrofa)
Authors: Karvelienė Birutė , Stadalienė Inga , Rudejevienė Jūratė , Burbaitė Evelina , Juodžentė Dalia , Masiulis Marius , Buitkuvienė Jūratė , Šakalienė Jurgita , Zamokas Gintaras
Published: January 2025

Leptospira is a bacteria responsible for a widespread zoonosis that affects both humans and animals. Leptospirosis is a challenging pathology to diagnose and treat since its signs are unspecific and symptoms vary greatly. The disease seems to be highly prevalent in environments where reservoir animals such as rats and small mammals are common. Even though leptospirosis in humans in Lithuania is rare, it remains a disease of significance in Europe. Information on reservoir animals and prevalence of Leptospira in wild animals in Lithuania is lacking. The aim of this country-wide study was to evaluate the seroprevalence of Leptospira in wild boars in Lithuania. Hunted animals were collected from ten counties that represented the boar population of the country. The sera of 451 collected boars were evaluated for eight Leptospira serovars using the microscopic agglutination test. Seropositivity was observed in 102 (22.6%) boars. Overall, 194 positive reactions occurred. Boars older than 2 years were affected by more serovars and were more seropositive than younger boars (p < 0.05). The highest number of positive reactions was observed in Panevėžys (87.9%) and Vilnius (69.1%) counties. The results of this study might indicate that the wild boar is a reservoir animal of Leptospira and plays a role in its transmission in Lithuania.
The Use of PediSTAT Application by Paramedics Working in Saudi Arabia to Reduce the Risk of Medication Error for Pediatric Patients
Authors: Alharthy Nesrin , Abuhaimed Raghad , Alturki Munirah , Alanazi Shatha , Althaqeb Raghad , Alghaith Alanowd , Alshibani Abdullah
Published: January 2025

Background/Objectives: This study aimed to assess and compare the rates of medication error (ME) using the PediSTAT application compared to the conventional method of calculating the correct dose and determining the appropriate route of medication administration for common pediatric emergencies. Methods: A prospective cross-sectional study design was used for the study. Data were collected using a questionnaire that was distributed to certified paramedics holding a bachelor’s degrees or higher and working in Riyadh City, Saudi Arabia. Alternate simple random sampling was used to recruit the participants into two groups using the same questionnaire: the PediSTAT group and the conventional method group. The questionnaire contained four pediatric emergency vignettes: cardiac arrest, asthma exacerbation, seizures, and hypoglycemia. Results: A total of 63 participants agreed to the study. Almost 80% of them were males, 81% held bachelor’s degrees, and 87% were certified in pediatric resuscitation courses. The findings of the study showed that the use of the PediSTAT application increased accuracy and reduced the risk of ME for common pediatric emergencies. This was shown to be statistically significant for asthma medication dose (p-value < 0.001, 95% CI 0.034–0.352), midazolam dose (p-value = 0.012, 95% CI 0.030–0.764), and hypoglycemia medication dose (p-value < 0.001, 95% CI 0.046, 0.452). Conclusions: The study findings supported the use of standardized precalculated applications such as PediSTAT, which was shown to reduce the risk of ME in prehospital care for pediatric emergencies.
Possibility of Using NO Modulators for Pharmacocorrection of Endothelial Dysfunction After Prenatal Hypoxia
Authors: Belenichev Igor , Popazova Olena , Yadlovskyi Oleh , Bukhtiyarova Nina , Ryzhenko Victor , Pavlov Sergii , Oksenych Valentyn , Kamyshnyi Oleksandr
Published: January 2025

Prenatal hypoxia (PH) is a key factor in the development of long-term cardiovascular disorders, which are caused by various mechanisms of endothelial dysfunction (ED), including those associated with NO deficiency. This emphasizes the potential of therapeutic agents with NO modulator properties, such as Thiotriazoline, Angiolin, Mildronate, and L-arginine, in the treatment of PH. Methods: Pregnant female rats were given a daily intraperitoneal dose of 50 mg/kg of sodium nitrite starting on the 16th day of pregnancy. A control group of pregnant rats received saline instead. The resulting offspring were divided into the following groups: Group 1—intact rats; Group 2—rat pups subjected to prenatal hypoxia (PH) and treated daily with physiological saline; and Groups 3 to 6—rat pups exposed to prenatal hypoxia and treated daily from the 1st to the 30th day after birth. Levels of sEPCR, Tie2 tyrosine kinase, VEGF-B, SOD1/Cu-Zn SOD, GPX4, and GPX1 in the heart’s cytosolic homogenate were assessed using ELISA. The expression of VEGF and VEGF-B mRNA was analyzed via real-time polymerase chain reaction, and the nuclear area of myocardial microvessel endothelial cells was evaluated morphometrically. Results: We have shown that only two representatives of this group—Angiolin and Thiotriazoline—are able to exert full effect on the indices of endothelial dysfunction after PH to decrease sEPCR, increase Tie-2, VEGF-B and VEGF-B mRNA, Cu/ZnSOD, and GPX in myocardial cytosol, and increase the area of endotheliocyte nuclei in 1- and 2-month-old rats in comparison with the control. Conclusions: Our results experimentally substantiate the necessity of early postnatal cardio- and endothelioprotection using NO modulators, taking into account the role of NO-dependent mechanisms in the pathogenesis of cardiovascular system disorders in neonates after PH.
Exploring the Underlying Mechanism of Weiling Decoction Alleviates Cold-Dampness Diarrhea Based on Network Pharmacology, Transcriptomics, Molecular Docking and Experimental Validation
Authors: Zhang Yannan , Zhang Shuai , Fan Yimeng , Huang Sijuan , Wang Shimin , Hao Zhihui , Shen Jianzhong
Published: January 2025

Background: Cold-dampness diarrhea (CDD) is a common gastrointestinal disorder in children, characterized by diarrhea and intestinal barrier dysfunction. Weiling decoction (WLD) is frequently used in clinical practice to treat CDD, a condition triggered by multiple factors. However, the molecular mechanisms underlying its therapeutic effects remain poorly understood. Objectives: This study aimed to evaluate the efficacy of WLD in treating CDD and to elucidate its potential mechanisms. Methods: UPLC-HRMS/MS was employed to identify the chemical constituents of WLD and the absorption components in the plasma of WLD-treated rats. Additionally, a rat model of CDD was established to assess the therapeutic effects of WLD through a comprehensive approach. To elucidate the molecular mechanisms underlying these effects, network pharmacology and transcriptomic analyses were performed to identify potential signaling pathways associated with CDD alleviation. Molecular docking and flow cytometry assays were subsequently utilized to validate the identified signaling pathways. Results: A total of 223 chemical components were detected in WLD, and 49 absorption components were identified in the plasma of WLD-treated rats by UPLC-HRMS/MS. WLD treatment significantly alleviated the symptoms of CDD, reduced intestinal damage, and diminished the inflammatory response. Additionally, WLD influenced key genes in immune-related pathways. Molecular docking revealed strong binding affinities between the main components of WLD and key targets within these pathways. Flow cytometry, along with the analysis of inflammatory cytokines and transcription factors, demonstrated that WLD modulated the balance between Th1/Th2 and Th17/Treg cell populations. Conclusions: This study provides the first evidence that WLD alleviates CDD by regulating the balance between Th1/Th2 and Th17/Treg cell populations. These findings offer a theoretical basis for future investigations into the therapeutic potential of WLD in the treatment of CDD.
Advancing the Taxonomy of the Diatom Pseudo-nitzschia Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea
Authors: Bonačić Tina , Arapov Jasna , Bušelić Ivana , Lepen Pleić Ivana , Milić Roje Blanka , Tomašević Tina , Bužančić Mia , Mladinić Marija , Casabianca Silvia , Penna Antonella , Skejić Sanda , Ninčević Gladan Živana
Published: January 2025

The marine diatom genus Pseudo-nitzschia comprises cosmopolitan phytoplankton species commonly present in the Adriatic Sea. Species within the genus Pseudo-nitzschia have been of significant concern because they produce domoic acid (DA), which can cause amnesic shellfish poisoning (ASP). In this study, we identified Pseudo-nitzschia species along the Central and Southeastern Adriatic Sea, where monthly sampling carried out from February 2022 to February 2024 allowed for comprehensive species documentation. Pseudo-nitzschia species cell cultures isolated from the study areas were morphologically and molecularly analysed. Morphological analyses were performed using a scanning electron microscope (FE-SEM/STEM), while molecular analyses were conducted, targeting the ITS1-5.8S-ITS2, LSU, and rbcL regions, to confirm species identity. This integrative approach led to the identification of eight species: Pseudo-nitzschia allochrona, Pseudo-nitzschia calliantha, Pseudo-nitzschia delicatissima, Pseudo-nitzschia fraudulenta, Pseudo-nitzschia mannii, Pseudo-nitzschia multistriata, Pseudo-nitzschia pseudodelicatissima, and Pseudo-nitzschia subfraudulenta. Our findings underscore the value of a combined approach for reliable species identification and contribute to the development of genetic sequence databases that support the advancement of next-generation methods such as metabarcoding. This research emphasises the importance of combined morphological and molecular methods for the differentiation of the cryptic and pseudo-cryptic Pseudo-nitzschia species.
Development of Thermoplastic Bi-Component Electrodes for Triboelectric Impact Detection in Smart Textile Applications
Authors: Esteves David Seixas , Melo Amanda , Peliteiro Bruno , Durães Nelson , Paiva Maria C. , Sequeiros Elsa W.
Published: January 2025

Smart textiles provide a significant technological advancement, but their development must balance traditional textile properties with electronic features. To address this challenge, this study introduces a flexible, electrically conductive composite material that can be fabricated using a continuous bi-component extrusion process, making it ideal for sensor electrodes. The primary aim was to create a composite for the filament’s core, combining multi-walled carbon nanotubes (MWCNTs), polypropylene (PP), and thermoplastic elastomer (TPE), optimised for conductivity and flexibility. This blend, suitable for bi-component extrusion processes, exemplifies the role of advanced materials in combining electrical conductivity, mechanical flexibility, and processability, which are essential for wearable technology. The composite optimisation balanced MWCNT (2.5, 5, 7.5, and 10 wt.%) and TPE (0, 25, and 50 wt.%) in a PP matrix. There was a significant decrease in electrical resistivity between 2.5 and 5 wt.% MWCNT, with electrical resistivity ranging from (7.64 ± 4.03)104 to (1.15 ± 0.10)10−1 Ω·m. Combining the composite with 25 wt.% TPE improved the flexibility, while with 50 wt.% TPE decreased tensile strength and hindered the masterbatch pelletising process. The final stage involved laminating the composite filament electrodes, with a 5 wt.% MWCNT/PP/(25 wt.% TPE) core and a TPE sheath, into a textile triboelectric impact detection sensor. This sensor, responding to contact and separation, produced an output voltage of approximately 5 V peak-to-peak per filament and 15 V peak-to-peak with five filaments under a 100 N force over 78.54 cm2. This preliminary study demonstrates an innovative approach to enhance the flexibility of conductive materials for smart textile applications, enabling the development of triboelectric sensor electrodes with potential applications in impact detection, fall monitoring, and motion tracking.
Thermosetting Resin for Plug and Abandonment of Oil Wells with Reduced Environmental Impact
Authors: Echarri-Giacchi Maria , Frederiksen Christian Husum , Skjolding Lars Michael , Skov Anne Ladegaard , Skowyra Magdalena
Published: January 2025

Plug and abandonment of offshore oil wells is a costly and time-consuming process, yet it is necessary for the ever-increasing number of mature fields in the region of the Danish North Sea, as well as globally. Current practices ensuring durable solutions for the complete zonal isolation of oil wells have a large environmental impact. This paper proposes a novel resin that could be mixed on the platform and pumped into the tubing in a liquid state. The increased temperature inside the oil well initiates the cross-linking reaction of the liquid resin, creating a solid and impermeable barrier. The liquid resin is thermally stable up to 180 °C and can be handled for up to 20 h at room temperature, preventing setting before intended while decreasing environmental impact. The solid resin has a compressive strength of 54 MPa and a steel adhesion strength of 6.27 MPa, highlighting its ability to withstand extreme downhole conditions.
Flexural Response Comparison of Nylon-Based 3D-Printed Glass Fiber Composites and Epoxy-Based Conventional Glass Fiber Composites in Cementitious and Polymer Concretes
Authors: Haibe Abdirahman Ahmed , Vemuganti Shreya
Published: January 2025

With 3D printing technology, fiber-reinforced polymer composites can be printed with radical shapes and properties, resulting in varied mechanical performances. Their high strength, light weight, and corrosion resistance are already advantages that make them viable for physical civil infrastructure. It is important to understand these composites’ behavior when used in concrete, as their association can impact debonding failures and overall structural performance. In this study, the flexural behavior of two designs for 3D-printed glass fiber composites is investigated in both Portland cement concrete and polymer concrete and compared to conventional fiber-reinforced polymer composites manufactured using a wet layup method. Thermogravimetric analysis, volume fraction calculations, and tensile tests were performed to characterize the properties of the fiber-reinforced polymer composites. Flexural testing was conducted by a three-point bending setup, and post-failure analysis was performed using microscopic images. Compared to concretes with no FRP reinforcement, the incorporation of 3D-printed glass-fiber-reinforced polymer composites in cementitious concrete showed a 16.8% increase in load-carrying capacity, and incorporation in polymer concrete showed a 90% increase in flexural capacity. In addition, this study also provides key insights into the capabilities of polymer concrete to penetrate layers of at least 90 microns in 3D-printed composites, providing fiber bridging capabilities and better engagement resulting in improved bond strength that is reflected in mechanical performance. The polymer material has a much lower viscosity of 8 cps compared to the 40 cps viscosity of the cement slurry. This lower viscosity results in improved penetration, increasing contact surface area, with the reinforcement consequently improving bond strength. Overall, this work demonstrates that 3D-printed fiber-reinforced polymer composites are suitable for construction and may lead to the development of advanced concrete-based reinforced composites that can be 3D-printed with tailored mechanical properties and performance.
Development of a Recycling Process for the Recovery of Gypsum Stone from Stockpile Material
Authors: Fenner Jacob , Luh Julius , Yagmurlu Bengi , Goldmann Daniel
Published: January 2025

Due to changes in the German government’s energy concept, the amount of gypsum produced in flue gas desulfurisation plants (FGD gypsum) will fall from 5 million tons per year to 1 million tons or less by 2038 at the latest. As of 2016, FGD gypsum accounts for 55% of German gypsum mix. The resulting raw material gap must be closed through innovative recycling concepts, such as the processing of existing mine dumps. The process development aims to achieve a calcium sulfate dihydrate content of 85% and a reduction in the stockpile volume by 50%. The main components of the stockpiles are calcium sulfate in the form of gypsum stone as well as clay minerals and organic matter. Successful laboratory tests were transferred to a pilot scale jigging machine with dewatering screening. The process water is circulated throughout the entire process. The gypsum content in the heavy fraction is 76% when measured with ICP OES and 87% when measured via thermogravimetric methods. Furthermore, pilot-scale dry screening on the stockpile took place, and up to 1500 tons of material could be processed. Due to fluctuating weather conditions, the screening quality was subject to significant variations. Under optimal conditions, up to 60% of the feed could be recovered as gypsum stone; however, the screening process was nearly impossible during rain; therefore, a process combination of screening and a downstream jigging machine is recommended.
The Sacred Architecture of Josep Lluís Sert
Authors: Ugalde-Blázquez Iñigo , Gómez-Val Ricardo , Lluis-Teruel Cinta , Moran-García Pilar
Published: January 2025

An unknown aspect of Josep Lluís Sert (Barcelona, 1902–1983) is his deep engagement with Christian spirituality, particularly following his American exile. This perspective is beautifully reflected in his religious-themed projects, among which historiography has highlighted the church of Puerto Ordaz (Venezuela, 1951), the Chapel of St. Botolph (Boston, 1963–1968), and the Carmel de la Paix Chapel (Mazille, 1967–1972), designed, respectively, before, during, and after the Second Vatican Council. Using these three well-known projects as a starting point, our aim is to expand the discussion around this topic to encompass the entirety of Sert’s sacred architecture. The contributions of Sert to the design of modern religious architecture are analyzed in this study, firstly through the distinctive aspects of his architecture, such as its urban scale and interactions between various plastic arts, and secondly through his theological references. This study is based on Sert’s original drawings, as well as specific bibliographic sources and articles from specialized journals. At the same time, it seeks to highlight an aspect of the architect that, despite the significance and brilliance of his designs, has received little attention until now.
Unravelling the Link Between Financialisation and Economic Growth: Evidence from Croatia
Authors: Mamuti Agim , Kadiu Fatbardha , Sherifi Idaver , Romānova Inna , Grima Simon
Published: January 2025

This study investigates the relationship between financialisation and economic growth in Croatia, focusing on the period from 1995 to 2021. Using time series econometric models, including the Augmented Dickey–Fuller test for stationarity, Johansen’s cointegration test for long-term relationships, and the Granger causality test within the Vector Error Correction Model (VECM) framework, the research reveals a sustained long-term equilibrium relationship between financialisation and economic growth in Croatia. However, the Granger causality test does not indicate a definitive causal direction between these variables. While the study is limited to the Croatian context and the specified period, its findings have significant implications for policymakers in Croatia and similar emerging markets. These results suggest that while financialisation can enhance economic growth through better resource allocation and increased investment, it may also pose risks such as financial instability. Such measures aim to mitigate the risks associated with financialisation while promoting sustainable economic growth. To address these challenges, we recommend the implementation of robust regulatory frameworks, financial literacy initiatives, and economic diversification strategies. Such measures aim to mitigate the risks associated with financialisation while promoting sustainable economic growth. The study fills an important research gap on financialisation in emerging markets, particularly in Croatia, providing empirical evidence on the long-term relationship between financialisation and economic growth and highlighting the need for context-specific policy interventions.
Deep Learning on 3D Semantic Segmentation: A Detailed Review
Authors: Betsas Thodoris , Georgopoulos Andreas , Doulamis Anastasios , Grussenmeyer Pierre
Published: January 2025

In this paper, an exhaustive review and comprehensive analysis of recent and former deep learning methods in 3D semantic segmentation (3DSS) is presented. In the related literature, the taxonomy scheme used for the classification of 3DSS deep learning methods is ambiguous. Based on the taxonomy schemes of nine existing review papers, a new taxonomy scheme for 3DSS deep learning methods is proposed, aiming to standardize it and improve the comparability and clarity across related studies. Furthermore, an extensive overview of the available 3DSS indoor and outdoor datasets is provided along with their links. The core part of this review is the detailed presentation of recent and former 3DSS deep learning methods and their classification using the proposed taxonomy scheme along with their GitHub repositories. Additionally, a brief but informative analysis of the evaluation metrics and loss functions used in 3DSS is included. Finally, a fruitful discussion of the examined 3DSS methods and datasets is presented to foster new research directions and applications in the field of 3DSS. In addition to this review, a GitHub repository is provided, including an initial classification of over 400 3DSS methods, using the proposed taxonomy scheme.
Barriers and Opportunities in Implementing Carbon Neutrality Goals in China’s Heavy Industries
Authors: Shao Bo , Zhang Liang , Shah Syed Ahsan Ali
Published: January 2025

The transition to carbon neutrality in China’s heavy industries is essential for mitigating global climate change, given the sector’s significant contribution to greenhouse gas emissions. This study systematically examines the multifaceted barriers and opportunities influencing decarbonization efforts in these industries. Employing expert-driven methodologies, including the Delphi method and the best-worst method (BWM), the research identifies and prioritizes critical challenges, such as the high upfront costs for renewable technologies, the technological dependency on coal, and the fragmented regulatory frameworks. It highlights transformative opportunities, emphasizing advancements in carbon capture, utilization, and storage (CCUS), renewable energy integration, and strengthened carbon pricing mechanisms. The findings reveal the interaction between economic, technological, and policy dimensions, underscoring the need for coordinated interventions to overcome entrenched barriers. The study contributes to theoretical advancements by integrating expert insights with robust multi-criteria decision-making techniques and offers actionable pathways for policymakers and industry stakeholders to accelerate industrial decarbonization. These insights align with international climate objectives, providing a scalable framework for global sustainable transitions in energy-intensive sectors.
Spatial Distribution and Dietary Risk Assessment of Aflatoxins in Raw Milk and Dairy Feedstuff Samples from Different Climate Zones in China
Authors: Yang Xueli , Liu Bolin , Zhang Lei , Wang Xiaodan , Xie Jian , Liang Jiang
Published: January 2025

This study aimed to explore the contamination of aflatoxins by investigating the spatial distribution of aflatoxin B1 (AFB1) in cow feedstuff and aflatoxin M1 (AFM1) in raw milk, and the potential health risks of AFM1 in milk and dairy products. Feedstuff and raw milk were collected from 160 pastures in three climate zones of China from October to November 2020. The results indicated the level of AFB1 and AFM1 ranged from 51.1 to 74.1 ng/kg and 3.0 to 7.0 ng/kg, respectively. Spatial analysis indicated the contamination was mostly concentrated in the temperate monsoon climate zone. On average, the estimated dietary exposure to AFM1 from milk and dairy products for Chinese consumers ranged from 0.0138 to 0.0281 ng/kg bw/day, with the MOE values below 10,000, and liver cancer risk of 0.00004–0.00009 cases/100,000 persons/year. For different groups, the average exposure to AFM1 was highest in the temperate monsoon climate zone and for toddlers.
Crustacean Zooplankton Ingestion of Potentially Toxic Microcystis: In Situ Estimation Using mcyE Gene Gut Content Detection in a Large Temperate Eutrophic Lake
Authors: Agasild Helen , Ferraz Margarita Esmeralda Gonzales , Saat Madli , Zingel Priit , Piirsoo Kai , Blank Kätlin , Kisand Veljo , Nõges Tiina , Panksep Kristel
Published: January 2025

Grazing by zooplankton can regulate bloom-forming cyanobacteria but can also transfer toxin-producing cells, as well as toxic metabolites, to the food web. While laboratory investigations have provided extensive knowledge on zooplankton and toxic cyanobacteria interactions, information on zooplankton feeding on toxin-producing cyanobacteria in natural water bodies remains scarce. In this study, we quantified Microcystis-specific mcyE synthase genes from the gut contents of various cladoceran and copepod taxa to assess the in situ crustacean community and taxon-specific ingestion of potentially toxic Microcystis in Lake Peipsi, a large eutrophic lake in Estonia, Northern Europe. Microcystis cells with mcyE genes were found in all crustaceans examined. However, some species, such as the cyclopoid copepod Mesocyclops leuckarti, were more efficient in ingesting potentially toxic Microcystis than other co-occurring cladocerans (Daphnia spp., Bosmina spp., Chydorus sphaericus) and copepods (Eudiaptomus gracilis). The amount of toxigenic Microcystis cells grazed by crustacean population changed temporarily, and copepods were the predominant consumers of toxigenic Microcystis during several months of the 5-month study period. Crustacean ingestion of toxigenic Microcystis was not related to Microcystis biomass or mcyE gene copy numbers in the environment but was instead related to the abundance of major crustacean grazers. Our findings emphasize the close interaction between crustacean zooplankton and toxigenic Microcystis, indicating that some species may play a more significant role in linking toxic cells within the food web than others.
The Global Challenge of Campylobacter: Antimicrobial Resistance and Emerging Intervention Strategies
Authors: Bukari Zubeiru , Emmanuel Toyin , Woodward Jude , Ferguson Richard , Ezughara Martha , Darga Nikhil , Lopes Bruno Silvester
Published: January 2025

Antimicrobial resistance (AMR) in Campylobacter species, particularly C. jejuni and C. coli, poses a significant public health threat. These bacteria, which are commonly found in livestock, poultry, companion animals, and wildlife, are the leading causes of foodborne illnesses, often transmitted through contaminated poultry. Extensive exposure to antibiotics in human and veterinary medicine creates selection pressure, driving resistance through mechanisms such as point mutations, horizontal gene transfer, and efflux pumps. Resistance to fluoroquinolones, macrolides, and tetracyclines complicates treatment and increases the risk of severe infections. Drug-resistant Campylobacter is transmitted to humans via contaminated food, water, and direct contact with animals, highlighting its zoonotic potential. Addressing this challenge requires effective interventions. Pre-harvest strategies like biosecurity and immune-based methods reduce bacterial loads on farms, while post-harvest measures, including carcass decontamination and freezing, limit contamination. Emerging approaches, such as bacteriocins and natural antimicrobials, offer chemical-free alternatives. Integrated, multidisciplinary interventions across the food chain are essential to mitigate AMR transmission and enhance food safety. Sustainable agricultural practices, antimicrobial stewardship, and innovative solutions are critical to curbing Campylobacter resistance and protecting global public health. Our review examines the dynamics of antimicrobial resistance in Campylobacter and presents current strategies to mitigate Campylobacter-related AMR, offering valuable insights for antimicrobial control in the poultry industry.
Scalar Field Kantowski–Sachs Solutions in Teleparallel F(T) Gravity
Authors: Landry Alexandre
Published: January 2025

In this paper, we investigate time-dependent Kantowski–Sachs spherically symmetric teleparallel F(T) gravity with a scalar field source. We begin by setting the exact field equations to be solved and solve conservation laws for possible scalar field potential, Vϕ, solutions. Then, we find new non-trivial teleparallel F(T) solutions by using power-law and exponential ansatz for each potential case arising from conservation laws, such as linear, quadratic, or logarithmic, to name a few. We find a general formula allowing us to compute all possible new teleparallel F(T) solutions applicable for any scalar field potential and ansatz. Then, we apply this formula and find a large number of exact and approximate new teleparallel F(T) solutions for several types of cases. Some new F(T) solution classes may be relevant for future cosmological applications, especially concerning dark matter, dark energy quintessence, phantom energy leading to the Big Rip event, and quintom models of physical processes.
Enhanced Immunogenicity and Affinity with A35R-Fc-Based Chimeric Protein Compared to MPXV A35R Protein
Authors: Bai Shimeng , Cui Yanxin , Liao Qibin , Yi Hongyang , Liao Zhonghui , Zhang Gengwei , Wu Fenfang , Lu Hongzhou
Published: January 2025

The re-emergence of the mpox pandemic poses considerable challenges to human health and societal development. There is an urgent need for effective prevention and treatment strategies against the mpox virus (MPXV). In this study, we focused on the A35R protein and created a chimeric A35R-Fc protein by fusing the Fc region of IgG to its C-terminal. We then assessed its reactivity with A35R-specific antibodies and human convalescent plasma, as well as its immunogenicity. Our findings indicate that the A35R-Fc protein significantly enhances affinity to A35R antibodies compared to the commercially available A35R protein and exhibits considerable reactivity to human plasma. Additionally, mice immunized with A35R-Fc exhibited increased neutralizing antibody titers against the live MPXV. These results support the potential of Fc domain chimeric antigens as a strategy to enhance the efficacy of subunit vaccines targeting the MPXV.
Transient Third-Degree Atrioventricular Block in a Dog with Addisonian Crisis
Authors: Pașca Paula Maria , Solcan Gheorghe , Blageanu Andrei , Lăcătuș Caroline Maria , Peștean Petru Cosmin , Stancu Constantin Adrian , Baisan Andrei Radu
Published: January 2025

A 3-year-old spayed male mixed-breed Labrador presented to the Emergency and Critical Care Unit with lethargy, loss of appetite, vomiting, a recent history of presyncopal episodes, and severe exercise intolerance. On admission, the patient had bradycardia, low blood pressure, and mild abdominal pain. Serum biochemistry information revealed severe hyperkalemia, hyponatremia, hypoglycemia, and mildly increased liver and kidney parameters. Upon taking these findings into consideration, hypoadrenocorticism was suspected, and a basal cortisol level was determined; it was below 1.0 µg/dL. Upon examination with ultrasound, we observed that the size of the adrenal glands was considerably reduced. Due to the bradycardia, a complete cardiologic examination consisting of 5 min of six-lead electrocardiography and echocardiography (ECG) was performed. Six-lead ECG revealed a complete atrio-ventricular block with an atrial rate of 140 bpm and a ventricular rate of 60 bpm. The PQ interval was variable, without any evidence of atrio-ventricular conduction. Treatment for hyperkalemia was initiated immediately after admission, followed by dexamethasone sodium phosphate administration, which led to an improvement in the patient’s general condition, and the normalization of the ECG readings, after 24 h of therapy. Afterward, prednisolone (0.5 mg/kg/24 h p.o.) and desoxycorticosterone pivalate (2.2 mg/kg s.c.) were administered. This case shows that a third-degree atrioventricular block might be a reversible problem in dogs with hypoadrecorticism that can be managed with specific treatment for this disorder, without requiring pacemaker implantation.
Geospatial and Multi-Criteria Analysis for Identifying Groundwater Potential Zones in the Oltu Basin, Turkey
Authors: Taşci Sait , Şenocak Serkan , Doğru Fikret , Wang Bangbing , Abdelrahman Kamal , Fnais Mohammed S. , El-Raouf Amr Abd
Published: January 2025

This study was conducted to determine potential groundwater storage areas in the semi-arid Oltu Basin in northeastern Turkey. The groundwater potential of the basin was analyzed by evaluating eight geographical factors: lithology, linear density, soil depth, land use, precipitation, geomorphology, slope, and drainage density. These factors were classified and weighted using remote sensing, geographical information systems (GIS), and the analytic hierarchy process (AHP). The obtained data were modeled using ArcGIS software, and a potential groundwater storage map of the Oltu Basin was created. The results show that there is a high groundwater potential in areas of the basin close to the stream bed, while the groundwater potential is low in mountainous and steeply sloped regions. The study provides significant findings for sustainable water resource management in the region and future water resources planning.
The Impacts of Different Salinities on the CW-MFC System for Treating Concentrated Brine
Authors: Wang Li , Han Xuwei , Zhang Yu , Wang Lin , Wang Jin
Published: January 2025

This paper aims to comprehensively explore the performance and influencing factors of the constructed wetland–microbial fuel cell (CW-MFC) system when treating brine with different concentrations. The main objective is to determine how different salinity levels affect the operation and treatment efficiency of the CW-MFC system. The research results show that Bruguiera gymnorrhiza exhibits strong salt tolerance and can be used as a wetland plant for the CW-MFC system. The closed-circuit CW-MFC system with planted plants has the best performance, with a chemical oxygen demand (COD) removal rate of 84.8%, a total nitrogen (TN) removal rate of 68.12%, and a chloride ion (Cl−) removal rate of 29.96%. The maximum power density is 64.79% higher than that of the system without planted plants. The power generation performance of the system first increases and then decreases with the increase in salinity, while the internal resistance keeps decreasing. When the salinity is 2%, the power generation effect is the best, with an average output voltage of 617.3 ± 25.7 mV and a power density of 45.83 mW/m2. The removal rates of COD and TN are inhibited with the increase in salinity, while the removal rate of total phosphorus (TP) is not significantly affected. The microbial community grows well under salt stress, but its structure is different. When the salinity is 1%, the optimal distance between electrodes is 10 cm. Considering the pollutant removal performance, the optimal hydraulic retention time is 3 days, and considering the power generation performance, the optimal hydraulic retention time is 2 days. This research provides important value for improving the performance of the CW-MFC system in treating brine.
How to organize the work of the admission department
Authors:   Alexandrovskaya Hospital, St. Petersburg, Safiullin M. S.
Published: January 2025

The admission department is a structural unit of a medical organization with a 24‑hour
hospital, consisting of medical premises and an administrative and economic part. It is intended
for registration, initial examination, anthropometry, sanitary and hygienic treatment of admitted patients, and provision of qualified (emergency) medical care. The basic principles of organizing the
work of the admission department are presented in the article.
How cloud services and other it tools affect enterprise management
Authors:   Mixbs LLC, Russia, Saratov, Sidorov S.B.
Published: January 2025

The article covers the issues of transformation of management processes in
enterprises under the influence of cloud technologies and modern IT tools. The author
analyzes how cloud computing, providing an on-demand computing model, changes
traditional approaches to data storage and IT infrastructure management. The evolution
of IT infrastructure is considered, emphasizing the advantages of cloud solutions, such
as scalability, cost reduction and simplification of collaboration, which are especially
relevant in the context of the spread of new strains of COVID-19 and other infectious
diseases.
Digital technologies in the field of labor protection and industrial safety
Authors:   KVAZAR LLC, Molokotin V.K.
Published: January 2025

The article considers the importance of maintaining the health of employees and
the use of modern technologies in the fi eld of medicine and industrial safety for eff ective risk
management. Implementation of an electronic medical examination system that automates
the process of medical examinations, management of health and labor protection processes
at the enterprise. Digital transformation in this area allows you to use the digital database,
optimize the organizational structure and create an eff ective and transparent interaction
system. Projects for the implementation of an electronic medical examination system have
been implemented in various industries, showing positive results in improving the safety
and health of workers. Examples of project implementation in the companies PJSC Uralkali,PJSC NLMK, PJSC MMC Norilsk Nickel, SIBUR Holding and PJSC Magnit describe the positive
changes that are observed after the implementation of the system. The implementation
of the EMS allows to increase the effi ciency of employee health monitoring, automate the
process of medical examinations, improve the safety of the working environment and create
a favorable working atmosphere.
Impact of digitalization in the formation of professional competencies of technical specialists
Authors:   Open Youth University, Tomsk, Russia, Dmitriyev I.V.
Published: January 2025

This article examines the impact of digitalization in the context of multi-level training
of technical specialists "School-university-enterprise", since the formation of professional
competencies of a specialist today is not limited to the framework of the university, but begins
long before admission. The article discusses a unique digital solution for building individual
educational trajectories for technical specialists starting from the school level.