bluuebunny/crossref_metadata_2025 huggingface dataset:
Defining “High-In” Saturated Fat, Sugar, and Sodium to Help Inform Front-of-Pack Labeling Efforts for Packaged Foods and Beverages in the United States
Authors: Dunford Elizabeth K. , Miles Donna R. , Hollingsworth Bridget A. , Heller Samantha , Popkin Barry M. , Ng Shu Wen , Taillie Lindsey Smith
Published: December 2024

Background: To help consumers make healthier choices, the US Food and Drug Administration (FDA) has been charged with developing a front-of-package label (FOPL) to appear on US packaged foods and beverages. One option being explored is the use of “high-in” FOPLs for added sugar, sodium, and saturated fat using a threshold of ≥20% of the recommended daily value (%DV) per portion/serving size to define “high-in”. While research has addressed what FOPL designs are most effective at visually communicating “high-in”, less attention has been paid to the nutrient profile model (NPM) used to decide which products should receive these labels. In addition, several established regional NPMs already exist that identify products that are high in nutrients of concern, but it is unclear how these compare to the FDA’s %DV approach. Methods: We used a dataset of 51,809 US products from Mintel’s Global New Products Database to examine how the FDA’s current definition of “high-in” compares to three established regional NPMs: the Canadian NPM, the Pan American Health Organization (PAHO) NPM, and Chile’s NPM. Results: Overall agreement between the four NPMs was 51% for foods and 72% for beverages, with highest agreement in categories such as sweetened sodas (87%), and lowest agreement in categories such as bread (14%) and salty snacks (29%). The Canadian NPM showed the highest agreement to the FDA “high-in” criteria while the Chilean and PAHO models had lower agreement. For many food categories, the FDA’s definition of “high-in” would require the fewest products to carry a “high-in” label. This issue was particularly pronounced in categories that tend to be served in small portions (e.g., salty snacks, bars), but disappeared or reversed for categories that are served in larger portions (e.g., frozen and non-frozen main dishes). Conclusions: The NPM chosen has important policy implications for an FOPL system’s ability to identify unhealthy foods and incentivize companies to reformulate products. Based on these results, the FDA should consider using a stronger NPM similar to those used elsewhere in the Americas region when deciding the final thresholds for “high-in” for US packaged foods and beverages.
Gestational Diabetes Mellitus, Breastfeeding, and Progression to Type 2 Diabetes: Why Is It So Hard to Achieve the Protective Benefits of Breastfeeding? A Narrative Review
Authors: Flores-Quijano María Eugenia , Pérez-Nieves Victor , Sámano Reyna , Chico-Barba Gabriela
Published: December 2024

Women diagnosed with gestational diabetes mellitus (GDM) face a significantly heightened risk of developing type 2 diabetes mellitus (T2DM) later in life. Breastfeeding (BF) has been identified as a potential strategy to delay or prevent T2DM; however, women with GDM often encounter barriers in initiating and maintaining adequate BF practices compared to those with uncomplicated pregnancies. This paradox prompts an exploration into the causes of these BF challenges and considers the possibility of reverse causation: Does prolonged and intensive BF mitigate the risk of subsequent glucose dysregulation and T2DM? Alternatively, do women with compromised insulin secretion and sensitivity, who are predisposed to T2DM, struggle to sustain intensive BF practices? This narrative review aims to explore the interplay between GDM, BF, and T2DM development by examining the different factors that present BF challenges among women with GDM. Understanding these dynamics is crucial for establishing realistic BF expectations and developing effective clinical and public health strategies to support BF in this high-risk population.
Association of Protein Intake with Sarcopenia and Related Indicators Among Korean Older Adults: A Systematic Review and Meta-Analysis
Authors: Han Minjee , Woo Kyungsook , Kim Kirang
Published: December 2024

Objectives: Due to variations in the standards for optimal protein intake and conflicting results across studies for Korean older adults, this study aimed to quantitatively integrate existing research on the association of protein intake with sarcopenia and related indicators in Koreans aged 65 and older through meta-analysis. Methods: A total of 23 studies were selected according to the study selection criteria (PICOS). Sixteen cross-sectional studies, 5 randomized controlled trials (RCTs), and 2 non-RCTs were included in the review, with 9 out of 23 studies included in the meta-analysis. We used fixed-effects models and performed subgroup and sensitivity analyses. Results: A meta-analysis found that the risk of sarcopenia was significantly higher in the <0.8 g/kg/day protein intake group compared to the 0.8–1.2 g/kg/day and ≥1.2 g/kg/day groups, with odds ratios (ORs) of 1.25 (95% confidence interval (CI), 1.10 to 1.42; I2 = 55%) and 1.79 (95% CI, 1.53 to 2.10; I2 = 71%), respectively. For low hand grip strength (HGS), the risk was higher in the <0.8 g/kg/day group compared to the 0.8–1.2 g/kg/day or ≥1.2 g/kg/day groups (OR 1.31; 95% CI, 1.03 to 1.65; I2 = 28%). No significant associations were found with other sarcopenia indicators, such as skeletal muscle mass, short physical performance battery score, balance test, gait speed, and timed up-and-go test. Conclusions: Lower protein intake is associated with a higher risk of sarcopenia and low HGS in Korean older adults. To establish protein intake recommendations for the prevention and management of sarcopenia in this population, further well-designed RCTs incorporating both protein supplementation and resistance training are necessary.
Impact of Work–Family Balance on Nurses’ Perceived Quality of Life During the COVID-19 Pandemic: A Scoping Review
Authors: Antolí-Jover Ana María , Gázquez-López María , Brieba-del Río Pascual , Pérez-Morente María Ángeles , Martín-Salvador Adelina , Álvarez-Serrano María Adelaida
Published: December 2024

Background/Objectives: The COVID-19 pandemic has put nurses under extreme pressure, especially affecting them by significantly increasing their workload and compromising their well-being. The lack of balance between work and personal life has caused greater stress and burnout in these professionals, deteriorating their quality of life and the care they provide. This study aims to synthesize the scientific evidence on the relationship between work–family balance and the perceived quality of life of nurses during the COVID-19 pandemic. Methods: A scoping review was carried out based on the Joanna Briggs Institute methodology, following the PRISMA-ScR guidelines in the PubMed, CINAHL, SCOPUS, WOS, Cochrane Library, and PsycINFO databases. The quality of the articles was assessed using the Mixed Methods Appraisal Tool (MMAT). Results: Five studies involving 1641 nurses from Spain, India, Jordan, South Korea, and Turkey focused on three key areas: (1) work–life balance, (2) quality of life, and (3) factors associated with quality of life during the COVID-19 pandemic. Conclusions: The pandemic exacerbated pre-existing challenges related to work–life balance and quality of life, particularly for nurses with rigid and extended work hours, increasing stress and negatively impacting their well-being. The study emphasizes the need for flexible labor policies, psychological support, and strong social networks to prevent burnout and improve nurses’ quality of life.
Susceptibility of Mouse Brain to MCMV Infection and Neuroinflammation During Ontogeny
Authors: Krstanović Fran , Mihalić Andrea , Šakota Lucija , Lisnić Berislav , Jonjić Stipan , Brizić Ilija
Published: December 2024

Human cytomegalovirus (HCMV) rarely infects the brain following infection of adult individuals. However, the virus readily infects the brain during congenital HCMV (cHCMV) infection, frequently causing severe neurodevelopmental and neurological sequelae. Interestingly, although the incidence of cHCMV infection is 0.5–1%, the proportion of congenitally infected individuals in which the virus manages to gain access to the brain is unknown. In this study, we used infection of mice with mouse cytomegalovirus (MCMV), the most commonly used experimental system for modeling HCMV disease in humans, to determine the impact of age on the susceptibility of the brain to cytomegalovirus infection and infection-mediated neuroinflammation. We demonstrate that infection of mice during various stages of neonatal development can lead to CMV neuroinvasion and inflammation. In contrast, MCMV infection does not result in MCMV neuroinvasion and neuroinflammation in weanling and adult mice. The obtained results establish a basis for elucidating the mechanisms of CMV neuroinvasion and the deleterious inflammatory response during ontogeny.
Pathology and VP2-Based Characterization of Infectious Bursal Disease Virus Associated with an Outbreak in Layer Chickens in Ghana
Authors: Enyetornye Ben , Abugri Henry A. , Kusi-Appiah Ama K. , Maboni Grazieli , Odoom Theophilus , Gottdenker Nicole L. , Velayudhan Binu T.
Published: December 2024

Infectious bursal disease (IBD) continues to threaten poultry production globally, with highly virulent strains circulating in many parts of Africa. In this study, molecular characterization was performed on a circulating infectious bursal disease virus (IBDV) strain from an outbreak in a layer flock in Ghana. Layer chicks presented for necropsy had markedly enlarged and hemorrhagic bursae of Fabricius, with necrotic foci and catarrhal exudate on the serosal surface. Histopathology of the bursa of Fabricius revealed scattered to effacing hemorrhages on the plicae, extensive necrosis with expansion of the stroma between the follicles, and depletion of lymphocytes within the interfollicular epithelium. Reverse transcription polymerase chain reaction (RT-PCR) and subsequent sequencing of the VP2 gene showed the presence of IBDV in formalin-fixed paraffin-embedded tissues. A phylogenetic analysis compared 62 other IBDV sequences from different parts of the world and placed the Ghanaian IBDV in genogroup 3 (vvIBDV), closely related to IBDV from Nigeria. In comparison to reference vvIBDV, there were amino acid substitutions at positions 252, 254, and 300. To the best of our knowledge, this is the first report in which an IBDV from a disease outbreak in Ghana has been sequenced and compared with other IBDVs in a phylogenetic analysis.
Strategies Used by SARS-CoV-2 to Evade the Innate Immune System in an Evolutionary Perspective
Authors: Fan Hong , Tian Mingfu , Liu Siyu , Ye Chenglin , Li Zhiqiang , Wu Kailang , Zhu Chengliang
Published: December 2024

By the end of 2019, the COVID-19 pandemic, resulting from the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), had diffused widely across the globe, with 770 million infected individuals and over 7 million deaths reported. In addition to its high infectivity and pathogenicity and its rapid mutation rate, the unique capacity of SARS-CoV-2 to circumvent the immune system has also contributed to the widespread nature of this pandemic. SARS-CoV-2 elicits the onset of innate immune system activation and initiates antiviral responses once it has infected the host. While battling the host’s immune responses, SARS-CoV-2 has established many countermeasures to evade attack and clearance. As the exploration of SARS-CoV-2 continues, substantial evidence has revealed that the 29 proteins synthesized by the SARS-CoV-2 genome are integral to the viral infection process. They not only facilitate viral replication and transmission, but also assist SARS-CoV-2 in escaping the host’s immune defenses, positioning them as promising therapeutic targets that have attracted considerable attention in recent studies. This review summarizes the manner in which SARS-CoV-2 interfaces with the innate immune system, with a particular focus on the continuous evolution of SARS-CoV-2 and the implications of mutations.
PPP2R5D-Related Neurodevelopmental Disorder and Multiple Haemangiomas: A Novel Phenotypic Trait?
Authors: Comisi Francesco , Soddu Consolata , Lai Francesco , Marica Monica , Lorrai Michela , Mancuso Giancarlo , Giglio Sabrina , Savasta Salvatore
Published: December 2024

Background: Houge-Janssens syndrome 1 is a condition with onset in early childhood caused by heterozygous pathogenic variants in the PPP2R5D gene, which encodes a B56 regulatory subunit of the serine/threonine protein phosphatase 2A (PP2A). There is evidence that the PP2A-PPP2R5D complex is involved in regulating the phosphatidylinositol 3-kinase (PI3K)/AKT signalling pathway, which is crucial for several cellular processes, including the pathogenesis and progression of haemangiomas. Case presentation: We report the first PPP2R5D-related neurodevelopmental disorder case from Sardinia, a child with transient hypoglycaemia, facial dysmorphisms, and multiple haemangiomas. Whole Exome Sequencing analysis confirmed the clinical suspicion, detecting the presence of the de novo missense variant c.592G>A in the PPP2R5D gene. Conclusions: Haemangiomas have never been linked to the syndromic phenotype of the PPP2R5D-associated disorder. The close correlation between the PP2A enzyme and the PI3K/AKT signalling pathway suggests the possible correlation between its dysfunction and activation of haemangiogenesis. Our report highlights a possible link between the PPP2R5D-related disorder and altered angiogenesis, characterizing diffuse haemangiomas as a possible novel phenotypic trait of this condition.
White Tea Reduces Dyslipidemia, Inflammation, and Oxidative Stress in the Aortic Arch in a Model of Atherosclerosis Induced by Atherogenic Diet in ApoE Knockout Mice
Authors: Huner Yigit Merve , Atak Mehtap , Yigit Ertugrul , Topal Suzan Zehra , Kivrak Mehmet , Uydu Huseyin Avni
Published: December 2024

Objective: In this study, we aimed to evaluate the potential effects of white tea (WT) in the atherosclerosis process characterized by oxidative stress, inflammation, and dyslipidemia. Methods: In our study, apolipoprotein E knockout (ApoE−/−) mice (RRID: IMSR_JAX:002052) and C57BL/6J mice (RRID: IMSR_JAX:000664) were used. In the atherosclerosis model induced by an atherogenic diet (AD), WT was administered via oral gavage at two different concentrations. The animals were sacrificed by decapitation under anesthesia, and their serum and aortic tissues were collected. Total cholesterol (TC), triglyceride (TG), interleukin (IL)-1β, IL-6, IL-10, IL-12, tumor necrosis factor-α (TNF-α), interferon-γ, myeloperoxidase, paraoxonase-1, lipoprotein-associated phospholipase A2, oxidized low-density lipoprotein (Ox-LDL), lectin-like oxidized LDL receptor (LOX-1), a disintegrin, and metalloprotease (ADAM) 10 and 17 activities were determined via colorimetric, enzyme-linked immunoassay, and fluorometric methods. Results: WT supplementation decreased serum Ox-LDL, LOX-1, TC, and TG levels by approximately 50%. TNF- and IL-6 levels were reduced by approximately 30% in the aortic arch. In addition, ADAM10/17 enzyme activities were found to be reduced by approximately 25%. However, no change in the AD-induced fibrotic cap structure was observed in the aortic root. Conclusions: The findings indicate that white tea effectively reduced oxidative stress, inflammation, and dyslipidemia in atherosclerosis but does not affect atheroma plaque morphology.
Immunoconjugates as an Efficient Platform for Drug Delivery: A Resurgence of Natural Products in Targeted Antitumor Therapy
Authors: Mihaylova Rositsa , Momekova Denitsa , Elincheva Viktoria , Momekov Georgi
Published: December 2024

The present review provides a detailed and comprehensive discussion on antibody–drug conjugates (ADCs) as an evolving new modality in the current therapeutic landscape of malignant diseases. The principle concepts of targeted delivery of highly toxic agents forsaken as stand-alone drugs are examined in detail, along with the biochemical and technological tools for their successful implementation. An extensive analysis of ADCs’ major components is conducted in parallel with their function and impact on the stability, efficacy, safety, and resistance profiles of the immunoconjugates. The scope of the article covers the major classes of currently validated natural compounds used as payloads, with an emphasis on their structural and mechanistic features, natural origin, and distribution. Future perspectives in ADCs’ design are thoroughly explored, addressing their inherent or emerging challenges and limitations. The survey also provides a comprehensive overview of the molecular rationale for active tumor targeting of ADC-based platforms, exploring the cellular biology and clinical relevance of validated tumor markers used as a “homing” mechanism in both hematological and solid tumor malignancies.
Salidroside: An Overview of Its Promising Potential and Diverse Applications
Authors: Liang Keke , Ma Shuhe , Luo Kai , Wang Renjie , Xiao Chenrong , Zhang Xianxie , Gao Yue , Li Maoxing
Published: December 2024

Salidroside, a phenolic compound isolated from various Rhodiola plants, is the principal active constituent of Traditional Chinese Medicine known for its adaptogenic properties. Due to the challenging environment of Rhodiola species, such as high altitude, high radiation, drought, and hypoxia, the source of salidroside is scarce. However, numerous studies have shown that salidroside has a range of biological activities, including cardiovascular and central nervous system activity, and anti-hypoxia, anti-inflammatory, and anti-aging activities. Although previous studies have partially summarized the pharmacological effects of salidroside, the overall pharmacological effects have not been analyzed. Hence, this review will systematically summarize the isolation, purification, synthesis, derivatization, pharmacological activity, pharmacokinetics, clinical application, and safety of salidroside. It is expected to provide new insights for the further research and pharmaceutical development of salidroside.
Testing the Validity of the Montgomery–Koyama–Smith Equation for Calculating the Total Petal Area per Flower Using Two Rosaceae Species
Authors: Zhao Chuanlong , Wang Jinfeng , Mu Youying , Yao Weihao , Wang Hui , Shi Peijian
Published: December 2024

The size of floral organs is closely related to the successful reproduction of plants, and corolla size is, to some extent, indicative of the size of floral organs. Petals are considered to be homologous to leaves, so we also attempted to estimate the area of a single petal using the method that is typically employed for estimating single leaf area (i.e., the Montgomery equation). Additionally, we estimated the total petal area per flower (AT; i.e., the whole corolla area) using the method designed for estimating the total leaf area per shoot (i.e., the Montgomery–Koyama–Smith equation). The Montgomery equation (ME) estimates the leaf area by assuming that the leaf area is proportional to the product of leaf length and width. The Montgomery–Koyama–Smith equation (MKSE) assumes that the total leaf area per shoot is proportional to the product of the sum of individual leaf widths and the maximum individual leaf length. To test the validity of the ME for predicting petal area, a total of 1005 petals from 123 flowers of two Rosaceae species, which exhibit a certain variation in petal shape, were used to fit the relationship between the petal area (A) and the product of petal length (L) and width (W). Two equations, including the MKSE and a power-law equation (PLE), were used to describe the relationship between the total petal area per flower and the product of the sum of individual petal widths and the maximum individual petal length. The root-mean-square error (RMSE) and the Akaike information criterion (AIC) were used to measure the goodness of fit and the trade-off between the goodness of fit and model’s structural complexity for each equation. The results show that the ME has a low RMSE value and a high correlation coefficient when fitting the relationship between A and LW for either of the two species. Additionally, the MKSE and the PLE exhibit low RMSEs and AICs for estimating the AT of both Rosaceae species. These results indicate that the ME, MKSE, and PLE are effective in predicting individual petal area and total corolla area, respectively.
Genome-Wide Association Study for Resistance to Phytophthora sojae in Soybean [Glycine max (L.) Merr.]
Authors: You Hee Jin , Zhao Ruihua , Choi Yu-Mi , Kang In-Jeong , Lee Sungwoo
Published: December 2024

Phytophthora sojae (Kauffman and Gerdemann) is an oomycete pathogen that threatens soybean (Glycine max L.) production worldwide. The development of soybean cultivars with resistance to this pathogen is of paramount importance for the sustainable management of the disease. The objective of this study was to identify genomic regions associated with resistance to P. sojae isolate 40468 through genome-wide association analyses of 983 soybean germplasms. To elucidate the genetic basis of resistance, three statistical models were employed: the compressed mixed linear model (CMLM), Bayesian-information and linkage disequilibrium iteratively nested keyway (BLINK), and fixed and random model circulating probability unification (FarmCPU). The three models consistently identified a genomic region (3.8–5.3 Mbp) on chromosome 3, which has been previously identified as an Rps cluster. A total of 18 single nucleotide polymorphisms demonstrated high statistical significance across all three models, which were distributed in eight linkage disequilibrium (LD) blocks within the aforementioned interval. Of the eight, LD3-2 exhibited the discernible segregation of phenotypic reactions by haplotype. Specifically, over 93% of accessions with haplotypes LD3-2-F or LD3-2-G displayed resistance, whereas over 91% with LD3-2-A, LD3-2-C, or LD3-2-D exhibited susceptibility. Furthermore, the BLINK and FarmCPU models identified new genomic variations significantly associated with the resistance on several other chromosomes, indicating that the resistance observed in this panel was due to the presence of different alleles of multiple Rps genes. These findings underscore the necessity for robust statistical models to accurately detect true marker–trait associations and provide valuable insights into soybean genetics and breeding.
Harnessing the Antibacterial, Anti-Diabetic and Anti-Carcinogenic Properties of Ocimum sanctum Linn (Tulsi)
Authors: Arya Rakesh , Faruquee Hossain Md. , Shakya Hemlata , Rahman Sheikh Atikur , Begum Most Morium , Biswas Sudhangshu Kumar , Apu Md. Aminul Islam , Islam Md. Azizul , Sheikh Md. Mominul Islam , Kim Jong-Joo
Published: December 2024

Ocimum sanctum Linn (O. sanctum L.), commonly known as Holy Basil or Tulsi, is a fragrant herbaceous plant belonging to the Lamiaceae family. This plant is widely cultivated and found in north-central parts of India, several Arab countries, West Africa and tropical regions of the Eastern World. Tulsi is known to be an adaptogen, aiding the body in adapting to stress by harmonizing various bodily systems. Revered in Ayurveda as the “Elixir of Life”, Tulsi is believed to enhance lifespan and foster longevity. Eugenol, the active ingredient present in Tulsi, is a l-hydroxy-2-methoxy-4-allylbenzene compound with diverse therapeutic applications. As concerns over the adverse effects of conventional antibacterial agents continue to grow, alternative therapies have gained prominence. Essential oils (EOs) containing antioxidants have a long history of utilization in traditional medicine and have gained increasing popularity over time. Numerous in vitro, in vivo and clinical studies have provided compelling evidence supporting the safety and efficacy of antioxidant EOs derived from medicinal plants for promoting health. This comprehensive review aims to highlight the scientific knowledge regarding the therapeutic properties of O. sanctum, focusing on its antibacterial, anti-diabetic, anti-carcinogenic, radioprotective, immunomodulatory, anti-inflammatory, cardioprotective, neurogenesis, anti-depressant and other beneficial characteristics. Also, the extracts of O. sanctum L. have the ability to reduce chronic inflammation linked to neurological disorders such as Parkinson’s and Alzheimer’s disease. The information presented in this review shed light on the multifaceted potential of Tulsi and its derivatives in maintaining and promoting health. This knowledge may pave the way for the development of novel therapeutic interventions and natural remedies that harness the immense therapeutic potential of Tulsi in combating various health conditions, while also providing valuable insights for further research and exploration in this field.
Aggressiveness of Puccinia striiformis f. sp. tritici Isolates at High Temperatures: A Study Case in Core Oversummering Area of Gansu as Inoculum Source
Authors: Zhang Bo , Zhao Jie , Huang Jin , Wang Xiaojie , Guo Zhijie , Jia Qiuzhen , Cao Shiqin , Sun Zhenyu , Luo Huisheng , Kang Zhensheng , Jin Shelin
Published: December 2024

Wheat stripe rust, caused by a biotrophic, obligate fungus Puccinia striiformis f. sp. tritici (Pst), is a destructive wheat fungal disease that exists worldwide and caused huge yield reductions during pandemic years. Low temperatures favor the development of the disease, but the global average temperature has been increasing since 1850, especially in China, which has a higher rising rate than the global average. In the last two decades, Pst isolates have shown increased aggressiveness under high temperatures. However, the effect of rising temperatures on the aggressiveness of Pst has remained unknown in China. Therefore, this study assessed the aggressiveness of 15 representative Pst isolates (6 new isolates collected before 2016 and 9 old isolates collected after 2016) in Gansu under high temperatures by measuring and comparing disease severity, spore germination, and latent period on wheat seedlings at 16 °C, 18 °C, and 22 °C. The results indicated that the six new isolates showed greater disease severity, higher spore germination ratio, and shorter latent period than the nine old isolates, indicating that the new isolates were more aggressive under high temperatures than the old isolates. Some new isolates, such as CYR34, CYR33, and CYR32, which are predominant, were inferred to be associated with high-temperature adaptation in addition to having more susceptible hosts. Our results provided an insight into changes in Pst isolates at warmer temperatures and increasing incidence of wheat stripe rust in China, especially in eastern sporadic epidemiological areas in recent years. Thus, the new isolates are likely to be a potential risk for causing increasing stripe rust incidence.
Essential Oils Obtained from Sicilian Citrus reticulata Blanco By-Products: Antibacterial and Allelopathic Activity
Authors: Geraci Anna , Postiglione Alessia , Sgadari Francesco , Schicchi Rosario , Badalamenti Natale , Bruno Maurizio , Basile Adriana , Dentato Martina , Maresca Viviana
Published: December 2024

Mandarin, one of the winter fruits commonly used in the preparation of foods and juices, is a fruit native to China and Southeast Asia. In this work, essential oils (EOs) obtained from by-products of the Citrus reticulata Blanco flavedo of five cultivars present and cultivated within the Botanical Garden of Palermo were chemically and biologically studied: C. reticulata ‘Avana’ (C1), C. reticulata ‘Tardivo di Ciaculli’ (C2), C. reticulata ‘Bombajensis’ (C3), C. reticulata ‘Aurantifolia’ (C4), and C. reticulata ‘Padre Bernardino’ (C5). The GC and GC-MS analysis performed on all the extracted samples clearly highlighted the notable presence of limonene, a characteristic hydrocarbon monoterpene of EOs of the Citrus genus. C1, C2, C3, C4, and C5 were tested in relation to their possible antibacterial and allelopathic activity, also highlighting the activity of limonene, the main compound. For the antibacterial activity, eight different bacterial strains were used, both Gram-positive and Gram-negative (Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, and Enterobacter aerogens). For the allelopathic effect, two model systems were chosen: the germination of radish seeds (Raphanus sativus L.) and of spores in the moss Tortula muralis (Hedw.). The EOs from all cultivars showed pronounced antibacterial effects against all strains with an MIC comprised in the range of 16–256 μg/mL. Limonene showed the highest activity with an MIC between 4 and 16. The allelopathic effects showed a decrease in the percentage of seed germination, root, and epicotyl growth on Raphanus and a strong reduction in the germination of Tortula spores with an alteration in the development of the protonema. Limonene showed the same but more intense allelopathic activity.
Assessment of Two Crosslinked Polymer Systems Including Hydrolyzed Polyacrylamide and Acrylic Acid–Hydrolyzed Polyacrylamide Co-Polymer for Carbon Dioxide and Formation Water Diversion Through Relative Permeability Reduction in Unconsolidated Sandstone Formation
Authors: Fakher Sherif , Khlaifat Abdelaziz , Mokhtar Karim , Abdelsamei Mariam
Published: December 2024

One of the most challenging aspects of manipulating the flow of fluids in subsurfaces is to control their flow direction and flow behavior. This can be especially challenging for compressible fluids, such as CO2, and for multiphase flow, including both water and carbon dioxide (CO2). This research studies the ability of two crosslinked polymers, including hydrolyzed polyacrylamide and acrylic acid/hydrolyzed polyacrylamide crosslinked polymers, to reduce the permeability of both CO2 and formation water using different salinities and permeability values and in the presence of crude oil under different injection rates. The result showed that both polymers managed to reduce the permeability of water effectively; however, their CO2 permeability-reduction potential was much lower, with the CO2 permeability reduction being less than 50% of the water reduction potential in the majority of the experiments. This was mainly due to the high flow rate of the CO2 compared to the water, which resulted in significant shearing of the crosslinked polymer. The crosslinked polymers’ swelling ratios were impacted differently based on the salinity, with the maximum swelling ratio being 9.8. The HPAM polymer was negatively affected by the presence of crude oil, whereas increasing salinity improved its performance greatly. All in all, both polymers had a higher permeability reduction for the formation water compared to CO2 under all conditions. This research can help improve the applicability of CO2-enhanced oil recovery and CO2 storage in depleted oil reservoirs. The ability of the crosslinked polymers to improve CO2 storage will be a main focus of future research.
From Experimentation to Optimization: Surface Micro-Texturing for Low-Friction and Durable PTFE–Steel Interfaces Under Full Film Lubrication
Authors: Long Risheng , Hou Jincheng , Zhang Yimin , Shang Qingyu , Ma Chi , Pape Florian , Marian Max
Published: December 2024

To enhance the sliding tribological performance between PTFE and 40#steel (AISI 1040) under full film lubrication conditions, laser surface texturing (LST) technology was employed to prepare micro-dimples on the contact surfaces of 40# steel discs. The Box–Behnken design response surface methodology (BBD-RSM) was applied to optimize the micro-dimple parameters. Coefficients of friction (COFs), wear losses and worn contact surfaces of the PTFE–40# steel tribo-pairs were researched through repeated wear tests, as lubricated with sufficient anti-wear hydraulic oil. The influencing mechanism of micro-dimples on the tribological behavior of tribo-pairs was also discussed. The results proved that micro-dimples can significantly improve the tribological properties of PTFE–40#steel tribo-pairs. The deviation between the final obtained average COF and the prediction by the BBD-RSM regression model was only 0.0023. Following optimization, the average COF of the PTFE–40# steel tribo-pair was reduced by 39.34% compared to the smooth reference. The wear losses of the PTFE ring and 40# steel disc decreased by 91.8% and 30.3%, respectively. This study would offer a valuable reference for the optimal design of key seals used in hydraulic cylinders.
Polymer Analysis and Characterization
Authors: Malucelli Giulio
Published: December 2024

This editorial aims to summarize some representative research efforts provided by the authors who contributed to the Polymer Analysis and Characterization section of the Polymers journal in the year 2024. The numerous and high-quality research outputs provided so far clearly indicate that the Polymer Analysis and Characterization section of the Polymers journal is rapidly and continuously growing, stimulating more and more researchers to publish their research outcomes here.
Antifungal Activity of Newly Formed Polymethylmethacrylate (PMMA) Modification by Zinc Oxide and Zinc Oxide–Silver Hybrid Nanoparticles
Authors: Mazur Marek Witold , Grudniak Anna , Szałaj Urszula , Szerszeń Marcin , Mizeracki Jan , Cierech Mariusz , Mierzwińska-Nastalska Elżbieta , Kostrzewa-Janicka Jolanta
Published: December 2024

Incorporating nanoparticles into denture materials shows promise for the prevention of denture-associated fungal infections. This study investigates the antifungal properties of acrylic modified with microwave-sintered ZnO-Ag nanoparticles. ZnO-Ag nanoparticles (1% and 2.5% wt.) were synthesized via microwave solvothermal synthesis (MSS). Nanoparticles were characterized for phase purity, specific surface area (SSA), density, morphology, and elemental composition. ZnO and ZnO-Ag nanoparticles were added to acrylic material (PMMA) at concentrations of 1% and 2.5% and polymerized. Pure PMMA (control) and obtained PMMA-nanocomposites were cut into homogeneous 10 × 10 mm samples. Antifungal activity of nanoparticles and PMMA-nanocomposites against C. albicans was tested using minimal inhibitory concentration (MIC) determination, and biofilm formation was assessed using crystal violet staining followed by absorbance measurements. Laboratory tests confirmed phase purity and uniform, spherical particle distribution. MIC results show antifungal activity of 1% Ag nanoparticles and the PMMA-2.5% (ZnO-1% Ag) nanocomposite. PMMA-1% (ZnO-1% Ag) nanocomposite and 1% ZnO-Ag nanoparticles are efficient in preventing biofilm formation. However, ZnO nanoparticles showed antibiofilm activity, and the PMMA-ZnO nanocomposite does not protect against biofilm deposition. Incorporating hybrid ZnO-Ag nanoparticles into PMMA is a promising antibiofilm method, especially with ZnO-1% Ag nanoparticles.
Residual Stress Analysis at the Conductor–Insulator Interface During the Curing Process of Hair-Pin Motors
Authors: Ma Mingze , Gan Hongyi , Shang Xiao , Song Linsen , Zhang Yiwen , Liu Jingru , Liu Chunbai , Hao Yanzhong , Zhang Xinming
Published: December 2024

The curing process of hair-pin motor stator insulation is critical, as residual stress increases the risk of partial discharge and shortens a motor’s lifespan. However, studies on the stress-induced defects during insulation varnish curing remain limited. This research integrates three-dimensional numerical simulations and experimental analysis to develop a curing model based on unsaturated polyester imide resin, aiming to explore the mechanisms of residual stress formation and optimization strategies. A dual fiber Bragg grating (FBG) sensor system is employed for simultaneous temperature and strain monitoring, while curing kinetics tests confirm the self-catalytic nature of the process and yield the corresponding kinetic equations. The multi-physics simulation model demonstrates strong agreement with the experimental data. The results show that optimizing the curing process reduces the maximum stress from 45.1 MPa to 38.6 MPa, effectively alleviating the stress concentration. These findings highlight the significant influence of the post-curing temperature phase on residual stress. The proposed model offers a reliable tool for stress prediction and process optimization in various insulating materials, providing valuable insights for motor insulation system design.
Enabling Manual Workplace Optimization Based on Cycle Time and Musculoskeletal Risk Parameters
Authors: Hanson Lars , Ljung Oskar , Högberg Dan , Vollebregt Janneke , Jiménez Sánchez Juan-Luis , Johansson Pierre
Published: December 2024

Recently the concept of Industry 5.0 has been introduced, reinforcing the human-centric perspective for future industry. The human-centric scientific discipline and profession ergonomics is applied in industry to find solutions that are optimized in regard to both human well-being and overall system performance. It is found, however, that most production development and preparation work carried out in industry tends to address one of these two domains at a time, in a sequential process, typically making optimization slow and complicated. The aim of this paper is to suggest, demonstrate, and evaluate a concept that makes it possible to optimize aspects of human well-being and overall system performance in an efficient and easy parallel process. The concept enables production planning and balancing of human work in terms of two parameters: assembly time as a parameter of productivity (system performance), and risk of musculoskeletal disorders as a parameter of human well-being. A software demonstrator was developed, and results from thirteen test subjects were compared with the traditional sequential way of working. The findings show that the suggested relatively unique parallel approach has a positive impact on the expected musculoskeletal risk and does not necessarily negatively affect productivity, in terms of cycle time and time balance between assembly stations. The time to perform the more complex two-parameter optimization in parallel was shorter than the time in the sequential process. The majority of the subjects stated that they preferred the parallel way of working compared to the traditional serial way of working.
Life Cycle Assessment of Biochar from Residual Lignocellulosic Biomass Using Kon-Tiki Kilns: Applications in Soil Amendment and Wastewater Filtration
Authors: Ramírez López Roxanna Pamela , Cabañas Vargas Diana , Aguilera-Cauich Erick Alberto , Sacramento Rivero Julio César
Published: December 2024

Producing biochar from residual biomass is an opportunity for health, environmental, and economic benefits to farmers in small traditional parcels, which are widespread in Latin America. This study presents a life cycle assessment of biochar in two circular economy scenarios: soil amendment and wastewater filtration. Seven mid-point environmental impact categories were assessed using the CML-IA method: acidification (AP), abiotic depletion (ADP), fossil fuels depletion (ADP-FF), eutrophication (EP), global warming (GWP), human toxicity (HTP), and smog formation (POCP). The soil amendment scenario showed lower impacts per tonne of biochar in all categories, especially for GWP (−801.3 kg CO2eq) and ADP-FF (−374.3 MJ), compared to the filtration scenario (−123.54 kg CO2eq and 827.85 MJ). Negative GWP values reflect reduced emissions from avoided fertilizers and carbon sequestration. However, POCP and HTP increased due to air emissions (CH4, NOx, NMVOC, and PM10) from the kiln. In both scenarios, biochar production contributed to 40–90% of the total impacts. Indirect emissions from electricity used for water pumping were identified as a hotspot in the filtration scenario.
Undisciplining the Science and Religion Discourse on the Holy War on Obesity
Authors: Gouw Arvin M.
Published: December 2024

Contemporary science and religion discourse (SRD) is a large field encompassing various topics, from creationism against evolution to theological anthropology and artificial intelligence, though historically, what is meant by “science” is Western science, and what is meant by “religion” is usually Christianity. Moreover, SRD has been driven mainly from the North American context. The scope of this paper will thus be more focused on Western science and North American Protestant Evangelical Christianity, which hereafter will be referred to as simply Christianity or religion. In this article, I argue that SRD often arises from conflict or intersections where such interdisciplinary dialogue is needed to better understand the topic. However, this also means that topics that seem to agree between religion and science are not discussed in SRD. It is as if the goal of SRD, consciously or unconsciously, is to attain some consensus. Topics that have achieved consensus are not worth interrogating using the interdisciplinary approach of SRD. In this article, I will raise the topic of the holy war on obesity as a case example. From the medical and scientific perspective, obesity is a significant epidemic and problem. Similarly, Christians also see obesity as a problem that their churches can help by reinforcing the need for self-control as a virtue. The alignment of the two fields leaves this subject primarily out of the radar of the academic SRD. Yet I argue here that this unholy alliance needs to be questioned because locating the solution to obesity simply on willpower to lose weight and battle gluttony is short-sighted at best, misleading perhaps, and harmful at worst. This paper calls for a transdisciplinary approach to the SRD on obesity, emphasizing the need to address the multifaceted nature of the problem, which spans physiology, psychology, sociology, economics, culture, and theology. In overlooking the complexity of the problem with its various intersectionalities, both science and religion in SRD have colonized bodies and health. Inherent within this transdisciplinary approach is the exercise of undisciplining SRD and decolonizing bodies. The concept of “undisciplining” involves re-evaluating the problem beyond mere weight loss, addressing interconnected issues such as food supply, government regulations, capitalism, discrimination, and mental health care. The narrative of gluttony as sin, the war metaphor, and the methodologies employed by both religious and scientific communities need to be deconstructed. In conclusion, recognizing the entangled system in which all are complicit, the paper advocates for a more nuanced and comprehensive approach, free from the constraints of traditional disciplinary boundaries and influenced narratives.
Understanding an Authoritarian Regime: The Varying Relations Between Religion and the State in East German Socialism, 1945–1989
Authors: Töpfer Jochen
Published: December 2024

The relations between religion and the state in the socialist-governed states of Eastern Europe in 1945–1989 are generally framed using the term “persecution of religion”. On the other hand, significant differences existed in the region, from limited freedom of religion in Yugoslavia to a prohibition of religious practice in Albania. From the side of religion, these relations were significantly formed by the attitudes and activities of religious leaders. Which options were adopted by key religious actors in a political environment hostile to religion? What is to be learned about religion in authoritarian systems? This qualitative study draws on the case of the German Democratic Republic (GDR), governed in 1949–1989 by the Socialist Unity Party of Germany. We studied professional biographies of prominent religious dignitaries and a comprehensive volume of files from the Ministry for State Security of the GDR in order to conceptualize their attitudes and activities according to a two-dimensional classification (towards the state and towards society), which enabled an overview of the range of their options, and identified the types of religious dignitaries in authoritarian systems. This analysis revealed that a variety of attitudes and activities of religious leaders existed in the GDR.
A Triple Threat: A Case Report Detailing Surgical Management for Hypertrophic Cardiomyopathy, Flail Mitral Valve and Severe Pulmonary Hypertension
Authors: Sunga Cass G. G. , Yang Kai-Chun , Oyetunji Shakirat , Swenson Erik R. , Khaira Kavita
Published: December 2024

The combination of hypertrophic cardiomyopathy with outflow tract obstruction, severe pre-capillary and post-capillary pulmonary hypertension, and severe primary mitral regurgitation is rare and presents distinct management challenges. Background and Clinical Significance: Pulmonary hypertension is an independent predictor of all-cause mortality in patients with hypertrophic cardiomyopathy managed medically and often precludes patients from undergoing cardiopulmonary bypass due to increased surgical morbidity and mortality. In studies specifically evaluating surgical myectomy, however, survival is favorable in patients with moderate-to-severe pulmonary hypertension. Case Presentation: We present a case of a 74-year-old male with six months of dyspnea with minimal exertion. A diagnostic work-up with transthoracic echocardiogram showed asymmetric left ventricular hypertrophy, left ventricular outflow tract obstruction with a peak gradient of 200 mmHg, right ventricular systolic pressure of 99 mmHg, systolic anterior motion of the mitral valve and flail anterior mitral leaflet. The patient was evaluated by a multi-disciplinary team and underwent extended septal myectomy and mitral valve repair with significant improvement in functional capacity post-operatively. Conclusions: While pulmonary hypertension increases the risk of morbidity and mortality during cardiopulmonary bypass, moderate-to-severe pulmonary hypertension in hypertrophic cardiomyopathy with outflow tract obstruction is a unique indication for septal reduction therapy that may not be associated with higher surgical mortality.
From Vineyard to Value: A Circular Economy Approach to Viticulture Waste
Authors: Provenzano Mariagrazia , Pacchera Francesco , Silvestri Cecilia , Ruggieri Alessandro
Published: December 2024

Due to its significant environmental impact, the wine industry is one of the most analyzed agri-food sectors in terms of sustainability. These include waste emission, intensive land use, and high transport-related carbon footprint. The circular economy represents an opportunity to mitigate these impacts by promoting the valorization of wine waste. Through a systematic literature review, this research provides an overview of studies from 2018 to 2024 that deal with the transformation of wine waste, in particular vinification residues, into resources. The research covers the commodity sectors in which wine waste is reused, such as Food, Agriculture, and Energy. The analysis of the sample reveals that the countries in which the issue of valorization of wine waste is most studied are Italy, Spain, and Portugal. The study encourages a better integration of waste in the wine sector into business practices by assessing the environmental and economic impact. From a management point of view, the aim is to inform companies about effective waste management practices in order to promote the development of a more sustainable wine industry. Future research will focus on evaluating the effectiveness of these valorization pathways, also through case studies of good practice in implementing a circular business model.