bluuebunny/crossref_metadata_2025 huggingface dataset:
Intratumoral androgen levels are linked to TMPRSS2-ERG fusion in prostate cancer
Authors: Knuuttila Matias , Mehmood Arfa , Mäki-Jouppila Jenni , Ryberg Henrik , Taimen Pekka , Knaapila Juha , Ettala Otto , Boström Peter J , Ohlsson Claes , Venäläinen Mikko S , Laiho Asta , Elo Laura L , Sipilä Petra , Mäkelä Sari I , Poutanen Matti
Published: May 2018

Intratumoral androgen biosynthesis is one of the mechanisms involved in the progression of prostate cancer, and an important target for novel prostate cancer therapies. Using gas chromatography-tandem mass spectrometry and genome-wide RNA sequencing, we have analyzed androgen concentrations and androgen-regulated gene expression in cancerous and morphologically benign prostate tissue specimens and serum samples obtained from 48 primary prostate cancer patients. Intratumoral dihydrotestosterone (DHT) concentrations were significantly higher in the cancerous tissues compared to benign prostate (P < 0.001). The tissue/serum ratios of androgens were highly variable between the patients, indicating individual patterns of androgen metabolism and/or uptake of androgens within the prostate tissue. An unsupervised hierarchical clustering analysis of intratissue androgen concentrations indicated that transmembrane protease, serine 2/ETS-related gene (TMPRSS2-ERG)-positive patients have different androgen profiles compared to TMPRSS2-ERG-negative patients. TMPRSS2-ERG gene fusion status was also associated with an enhanced androgen-regulated gene expression, along with altered intratumoral androgen metabolism, demonstrated by reduced testosterone concentrations and increased DHT/testosterone ratios in TMPRSS2-ERG-positive tumors. TMPRSS2-ERG-positive and -negative prostate cancer specimens have distinct intratumoral androgen profiles, possibly due to activation of testosterone-independent DHT biosynthesis via the alternative pathway in TMPRSS2-ERG-positive tumors. Thus, patients with TMPRSS2-ERG-positive prostate cancer may benefit from novel inhibitors targeting the alternative DHT biosynthesis.
Resistin inhibits neuronal autophagy through Toll-like receptor 4
Authors: Miao Jie , Benomar Yacir , Al Rifai Sarah , Poizat Ghislaine , Riffault Laure , Crépin Delphine , Taouis Mohammed
Published: May 2018

Autophagy is a non-selective degradation pathway induced in energy-deprived cells and in non-starved cells by participating in cellular inflammatory responses mainly through the elimination of injured and aged mitochondria that constitute an important source of reactive oxygen species. We have previously reported that resistin/TLR4 signaling pathway induces inflammation and insulin resistance in neuronal cell. However, the impact of resistin-induced inflammation on neuronal autophagy is unknown. In the present study, we hypothesized that resistin-induced neuroinflammation could be attributed, at least partially, to the impairment of autophagy pathways in neuronal cells. Our data show that resistin decreases neuronal autophagy as evidenced by the repression of the main autophagy markers in SH-SY5Y human neuroblastoma cell line. Furthermore, the silencing of TLR4 completely abolished these effects. Resistin also inhibits AMPK phosphorylation and increases that of Akt/mTOR contrasting with activated autophagy where AMPK phosphorylation is augmented and mTOR inhibited. In vivo, resistin treatment inhibits the mRNA expression of autophagy markers in the hypothalamus of WT mice but not in Tlr4−/− mice. In addition, resistin strongly diminished LC3 (a marker of autophagy) labeling in the arcuate nucleus of WT mice, and this effect is abolished in Tlr4−/− mice. Taken together, our findings clearly reveal resistin/TLR4 as a new regulatory pathway of neuronal autophagy.
Successful Surgical Treatment of Descending Aorta Interruption: A Case Report
Authors: Zhu Pengxiong , Ye Xiaofeng , Zhao Qiang
Published: May 2018

Descending aorta interruption is an extremely rare congenital defect. Conventional repair with end-to-end anastomoses is often a surgical challenge in view of the extensive collateral vessels that develop on the chest wall and inside the chest cavity. Extra-anatomic bypass is the preferred technique for the surgical repair of this entity, which avoids the network of collateral vessels, enables simultaneous treatment of associated lesions, and in all likelihood reduces morbidity and mortality. Here we describe an extra-anatomic bypass from the ascending aorta to the bilateral iliac arteries in a 24-year-old woman using vascular grafts (MAQUET Holding B.V. & Co. KG, Rastatt, Germany) without cardiopulmonary bypass.
Prospective, Randomized Un-Blinded Three Arm Controlled Study in Coronary Artery Revascularization with Minimal Invasive Extracorporeal Circulation Systems (MiECC): Surrogate Parameter Analysis of Biocompatibility
Authors: Kiessling Arndt H , Keller Harald , Moritz Anton
Published: May 2018

Objectives: Minimal extracorporeal circulation techniques and systems (MiECC) may reduce the negative side effects of conventional extracorporeal circulation (ECC). However, it is still unclear as to what this is caused by, the reduced priming volume and hemodilution, or the avoidance of blood-air contact and dispersion of mediastinal debris into the systemic circulation. The aim of the trial was the comparison of MiECC to an open ECC setup (openECC) or a system with reduced blood air and debris interaction (closeECC). Methods: In a prospective randomized trial, 72 patients (73 ± 5.3 years; 83% male) referred for coronary artery bypass graft (CABG) were randomly assigned either to MiECC (priming volume 550mL), closeECC, or openECC (priming volume 1250mL). The laboratory surrogate endpoints (renal function, inflammatory response, ischemia, coagulation, and hemolysis) and clinical data were measured at six different time points (T1-6). Results: Patients were comparable for all preoperative variables. The operation times (MiECC 261 ± 79min; openECC 264 ± 75min; closeECC 231 ± 68min) and perfusion times (MiECC 115 ± 49min; openECC353 107 ± 37min; closeECC 99 ± 22min) revealed a trend of faster performance in the closeECC group (P < .05). Pro-inflammatory cytokines, ischemia, and coagulation markers were significantly elevated postoperatively in all cardiopulmonary bypass types, and decreased to pre-baseline levels at discharge (T5) without identifiable statistical differences between the three study groups. Free-hemoglobin was not significantly increased by centrifugal pump or cell saver procedures. Significant intraoperative hemodilution effects due to the different priming volumes were demonstrated only at the end of operation (T2) (MiECC Hb 9.6 ± 1.1g/dL; openECC Hb 9.0 ± 0.8g/dL; closeECC Hb 8.7 ± 1g/dL; P =. 01).Conclusion: Neither the hemodilution, suction technique (MiECC), nor blood-air interface (closeECC) could show sustainable benefits in this underpowered study, compared to conventional ECC systems (openECC) in a high volume series of surrogate parameters.
Establishment of Locally Adapted Mutations Under Divergent Selection
Authors: Tomasini Matteo , Peischl Stephan
Published: May 2018

We study the establishment probabilities of locally adapted mutations using a multi-type branching process framework. We find a surprisingly simple and intuitive analytical approximation for the establishment probabilities in a symmetric two-deme model under the assumption of weak (positive) selection. This is the first analytical closed-form approximation for arbitrary migration rate to appear in the literature. We find that the establishment probability lies between the weak and the strong migration limits if we condition the origin of the mutation to the deme where it is advantageous. This is not the case when we condition the mutation to first occur in a deme where it is disadvantageous. In this case we find that an intermediate migration rate maximizes the probability of establishment. We extend our results to the cases of multiple demes, two demes with asymmetric rates of gene flow, and asymmetric carrying capacities. The latter case allows us to illustrate how density regulation can affect establishment probabilities. Finally, we use our results to investigate the role of gene flow on the rate of local adaptation and identify cases in which intermediate amounts of gene flow facilitate the rate of local adaptation as compared to two populations without gene flow.
Cognitive Development and Quality of Life Associated With BPD in 10-Year-Olds Born Preterm
Authors: Sriram Sudhir , Schreiber Michael D. , Msall Michael E. , Kuban Karl C.K. , Joseph Robert M. , O’ Shea T. Michael , Allred Elizabeth N. , Leviton Alan ,   for the ELGAN Study Investigators
Published: May 2018

OBJECTIVES:

To compare neurocognitive, language, executive function, academic achievement, neurologic and behavioral outcomes, and quality of life at age 10 years in children born extremely preterm who developed bronchopulmonary dysplasia (BPD) to children who did not develop BPD.

METHODS:

The Extremely Low Gestational Age Newborns study population included 863 children born extremely preterm whose BPD status before discharge was known had an IQ (Differential Ability Scales II [DAS II]) assessment at 10 years. We evaluated the association of BPD with any cognitive (DAS II), executive function (NEuroPSYchological Assessment II), academic achievement (Wechsler Individual Achievement Test-III and Oral and Written Language Scales [OWLS]) as well as social dysfunctions (Social Responsiveness Scale). We used logistic regression models, adjusting for potential confounding factors, to assess the strength of association between the severity of BPD and each outcomes.

RESULTS:

Three hundred and seventy-two (43%) children were oxygen-dependent at 36 weeks postconception age, whereas an additional 78 (9%) were also oxygen- and ventilator-dependent. IQ scores 2 or more SDs below the expected mean (ie, z scores ≤−2) occurred twice as commonly among children who had BPD as among those who did not. Children with severe BPD consistently had the lowest scores on DAS II, OWLS, Wechsler Individual Achievement Test-III, NEuroPSYchological Assessment II, and Social Responsiveness Scale assessments.

CONCLUSIONS:

Among 10-year-old children born extremely preterm, those who had BPD were at increased risk of cognitive, language, and executive dysfunctions; academic achievement limitations; social skill deficits; and low scores on assessments of health-related quality of life.
Diseño independiente: estrategias de singularización de un profesional
Authors: Dorado Velasco María Ximena
Published: May 2018

El presente artículo recoge cuestiones generales de una investigación sobre el diseño independiente en Bogotá y expone ciertas apreciaciones de la independencia como un lugar de enunciación subjetivo que motivó nuevos procesos de singularización en respuesta a las formas hegemónicas e institucionalizadas de la vida social. Mi interés por esa temática se derivó del carácter tanto indefinido como inestable de la independencia y de percibir allí definiciones seductoras de los modos de ser diseñador y hacer diseño que conquistaban cada vez más adeptos en el ámbito ocupacional: jóvenes profesionales convencidos de querer ser su propio jefe, de trabajar sin horarios, de vivir de una pasión y de ser absolutamente responsables de su desenlace. En nombre de la independencia estos sujetos activaban un modelo productivo y de valor que provocaba tensiones y desencuentros con formas de acción institucionalizadas y que, a su vez, los llevaba a luchar incansablemente por recibir atención y reconocimiento.
Diseño de herramientas precisas usadas con las manos: seguimiento ocular y otras técnicas de usabilidad
Authors: Puentes-Lagos David Ernesto , Mojica Hernan Ricardo , Arango Vásquez María Alejandra
Published: May 2018

Este artículo presenta las conclusiones de una investigación cuyo objetivo fue caracterizar atributos de diseño que permitan a creativos y otras partes interesadas desarrollar asertivamente un dispositivo de precisión usado con las manos. Para ello, se tuvieron en cuenta las técnicas de la usabilidad como los test y el seguimiento ocular, y se puo en práctica en un ejercicio con cuarenta usuarios que probaron cinco dispositivos representativos de las herramientas de precisión operadas con las manos: destornillador, esfero, llave, pincel y dosificador, por medio de la ejecución de tres procesos: reconocer el objeto, hacer una tarea real y otras controladas. Se concluyó que las personas muestran una curva de aprendizaje a medida que repiten la tarea solicitada, y que la configuración y la morfología de los sólidos para el agarre de los objetos precisos, influye en el desempeño de las mismas. Las herramientas con morfologías complejas presentaron mayor cantidad de visualizaciones, mientras que el diseño adecuado del sistema de control o mango de las herramientas de precisión requiere de superficies fáciles de identificar y que se parezcan a herramientas similares de la vida cotidiana.
¿Un intento epistemológico? El diseño como vigilante de métodos
Authors: Pérez García David
Published: May 2018

La epistemología del diseño ha sido objeto de numerosos debates. En este trabajo se expondrá la posibilidad de entender que el diseño tiene una especificidad que no se puede confundir con otro aspecto de las actividades humanas como la ciencia, el arte y la técnica. Con base en los postulados de algunos científicos sociales, se propone una estructura cognitiva para no hablar de un objeto de conocimiento, dado que el diseño no es ciencia ni epistemología. Así, se consolida la posibilidad de comprender que la especificidad de los elementos de diseño se encuentra entre lo sensible y lo inteligible, pues comparte elementos del conocimiento con la ciencia y lo sensible con el arte, pero sin tener relación directa con dichas actividades. Desde luego, la vigilancia del método se trató de abstraer de la propuesta para demostrar que los elementos tienen una duplicidad, en cuanto actuación en la vida cotidiana desde la aplicación de los resultados de la ciencia para hacerlos sensibles en distancia con el arte.
El oficio del sastre
Authors: Rodríguez Peralta Camilo Andrés
Published: May 2018

La brecha histórica que separa el arte, el diseño y la artesanía ha distanciado la teoría del ejercicio práctico, degradando, por ejemplo, el conocimiento instalado en la experiencia del artesano. Por esa razón, el propósito de esta investigación es el reconocimiento de los elementos que revelan la existencia de pensamiento, concepto y práctica de diseño en el oficio del sastre en la ciudad de Bogotá. Para ello, fueron seleccionados cinco maestros de sastrería –con más de veinte años de experiencia en el oficio– para indagar acerca del proceso de ideación-creación de un traje. Cada caso fue analizado bajo la estructura teórica del proceso iterativo de diseño, contrastando en cada uno la presencia de las fases características del modelo teórico de diseño: análisis, síntesis, proyección y comunicación como categorías para la identificación de patrones. Los resultados revelan la existencia de elementos de diseño en el oficio del sastre al presentar rasgos propios de cada etapa del proceso iterativo de diseño, así como actitudes y acciones que conducen a la construcción de un conjunto de nociones acerca de los usuarios, las acciones y los escenarios de su práctica.
Las imágenes enseñan desequilibrando. Televisión educativa colombiana
Authors: Rojas Torres Olga
Published: May 2018

El pensamiento está estructurado como una red que tiende al equilibrio. Sin embargo, para que se produzca el aprendizaje es necesario desequilibrar la malla cognitiva. Así, nos preguntamos cómo provocar desequilibrios. Para responder esta inquietud estudiamos dos series de televisión educativa infantil animada colombiana que circularon por el canal Señal Colombia en 2010: Jaibaná y Wanana. El análisis se hizo siguiendo las pautas de la semiolingüística. La pregunta planteada al análisis semiolingüístico del aparato argumentativo de las series es si se producen y cómo producen imágenes que revelen relaciones lógicas. Para hacer el puente entre el lenguaje visual y la cognición se tomaron los conceptos de choque y encadenamiento en la edición de cuadros y secuencias de imagen. Para el campo de cognición las guías fueron los conceptos de argumentación, equilibrio y desequilibrio. Si bien el análisis arrojó como tendencia el uso de imágenes tipo encadenamientos y secuencias asociables a la lógica de causa-consecuencia, en este artículo se enfatiza en las cualidades desequilibrantes de la imagen y sus recursos. Así, tenemos secuencias lógicas equilibradas o desequilibradas, siendo estas últimas las de mayor impacto educativo. También encontramos imágenes que contienen lo que podríamos llamar argumento y contrargumento en un solo cuadro.
Acerca del concepto de memoria gráfica
Authors: Lena Farias Priscila
Published: May 2018

En Brasil, desde principios del siglo XXI, la expresión memoria gráfica ha sido usada, cada vez con más frecuencia, para denominar una línea de estudios que busca comprender la importancia y el valor de los artefactos visuales, en particular, los impresos efímeros, para la creación de un sentido de identidad local a través del diseño. Tales artefactos han sido invisibilizados por mucho tiempo por las corrientes dominantes sobre lo que sería el diseño y, en consecuencia, de lo que debe hacer parte de la historia del diseño. Otras historias del diseño que incluyan tales artefactos aún deben ser descubiertas y narradas.Este artículo propone una reflexión acerca de los contextos en los que ha sido utilizada la memoria gráfica en el campo de la investigación de los artefactos visuales en Brasil, partiendo de una discusión sobre las conexiones posibles con conceptos más conocidos, como los de cultura de la impresión, cultura visual y cultura material, dando ejemplos de investigaciones realizadas, y llegando a una propuesta de cómo la idea de memoria gráfica puede ser adoptada como una estrategia para abordar el descubrimiento o la construcción de historias de diseño gráfico y visual en países no hegemónicos.
Repercussions of hospitalization due to fall of the elderly: health care and prevention
Authors: Luzardo Adriana Remião , Paula Júnior Newton Ferreira de , Medeiros Marcelo , Wolkers Paula Carolina Bejo , Santos Silvia Maria Azevedo dos
Published: May 2018

ABSTRACT Objective: To know the repercussions of the fall reported by the elderly and their caregiver during hospitalization in a public hospital in Florianópolis city from October to December 2014. Method: Exploratory research with a qualitative approach, conducted by depth interviews with 16 participants, the eight elderly were hospitalized for falls and eight elderly caregivers. Data analysis were performed through the Thematic Content Analysis. Results: It was evidenced the thematic axis: Faller Elderly supported by four thematic categories: Changes caused by Falls, I am a faller, I take care of me and Prevention of the Fall. The repercussions of the fall were evidenced in the impairment of the health condition, self-care and functional capacity. We observed the naturalization of the phenomenon and the passivity with the harmful consequences of the event. Final Considerations: The fall is valued the more negative its repercussion, such as the need for hospitalization and surgery. Managing the vulnerability of the elderly, especially in primary care, evaluating their comorbidities and their internal and external environment, will minimize unfavorable consequences and the social and financial cost of hospitalizations.
Temporal analysis of the functional status of older people in the state of Paraíba, Brazil
Authors: Freitas Fabiana Ferraz Queiroga , Beleza Cinara Maria Feitosa , Furtado Isadora Queiroz Correa Garchet , Fernandes Aline da Rocha Kallás , Soares Sônia Maria
Published: May 2018

ABSTRACT Objective: To verify the profile and the functional status of older people living in the state of Paraíba, Brazil, from a temporal perspective. Method: This was a descriptive study with secondary analysis of data from the Health Indicator and Aging Policy Monitoring system (SISAP-Idoso – Sistema de Indicadores de Saúde e Acompanhamento de Políticas do Idoso) between 2000 and 2010. Results: Over the analyzed period, there was a growth of older women, people older than 85 years, residents of urban areas, older people who live alone and who are not responsible for the household. There was also a decrease of illiterate older people, with monthly income of up to one minimum wage and in poverty situations. Concerning the functional status, the proportion of older people who reported any permanent mental, motor, visual or hearing disabilities has increased. Conclusion: We suggest that the assistance must be directed towards environmental variables that can influence the functional state, such as illiteracy, low income and disabilities that contribute to the weakening of older people and must be overcome.
Self-perceived health and clinical-functional vulnerability of the elderly in Belo Horizonte/Minas Gerais
Authors: Ribeiro Edmar Geraldo , Matozinhos Fernanda Penido , Guimarães Gilberto de Lima , Couto Alcimar Marcelo do , Azevedo Raquel Souza , Mendoza Isabel Yovana Quispe
Published: May 2018

ABSTRACT Objective: To determine the self-perceived health status and clinical-functional vulnerability of the elderly attended at a Reference Center of Minas Gerais, Brazil and to evaluate the association between these variables through the Clinical-Functional Vulnerability Index (IVCF-20) instrument. Method: This is an epidemiological, retrospective study of 311 medical records. Statistical analyses were performed using the Stata program; the evaluations were by Pearson’s Chi-square test and Poisson regression models. Results: The majority of the elderly presented negative self-perceived health status(70.10%); there was statistical significance between negative self-perceived health and the variables of mood and recent hospitalization. Conclusion: Perceived health status influences the morbidity and mortality of the elderly. Mood disorders and recent hospitalizations directly interfere with active aging.
Validity and reliability of the Brazilian version of the Caregiver Reaction Assessment
Authors: Mota Fernanda Rochelly do Nascimento , Victor Janaína Fonseca , Silva Maria Josefina da , Soares Edson Silva , Oriá Mônica Oliveira Batista , Marques Marília Braga , Freitas Maria Célia de , Alves Allana Mirella
Published: May 2018

ABSTRACT Objective: To analyze the validity based on the internal structure and reliability of the Brazilian version of the Caregiver Reaction Assessment (CRA) applied to informal caregivers of dependent elderly. Method: A methodological study with 120 informal caregivers of the elderly. Validity based on the internal structure evaluated by exploratory factorial analysis, with extraction of main factorial axes, varimax orthogonal rotation and retention of factors by parallel analysis. Reliability assessed by Cronbach’s alpha. Results: The instrument revealed a factorial structure different from the original version: three factors were identified, equivalent to subscales, self-esteem, daily activities and family support, with explained variance of 42%. The values of the Cronbach alpha for the items belonging to each subscale were, respectively: 0.71, 0.81 and 0.83. Conclusion: The Brazilian version of CRA presents good evidence of validity based on internal structure and is reliable, and can be used to assess the overload of informal caregivers of dependent elderly.
Self-care of elderly people after the diagnosis of acquired immunodeficiency syndrome
Authors: Araujo Graciela Machado de , Leite Marinês Tambara , Hildebrandt Leila Mariza , Oliveski Cinthia Cristina , Beuter Margrid
Published: May 2018

ABSTRACT Objective: to characterize the seropositive elderly for the Human Immunodeficiency Virus and Acquired Immunodeficiency Syndrome (HIV/AIDS) in their socio-demographic aspects; to understand how the elderly take care of themselves from the diagnosis of HIV/AIDS. Method: Qualitative, descriptive, exploratory research conducted at a Voluntary Counseling and Testing Center with 10 elderly people receiving treatment for HIV/AIDS. The data were analyzed according to the content analysis. Results: Data show the elderly people’s lack of knowledge about HIV/AIDS transmission, the experience of being elderly and having HIV/AIDS, caring for oneself and life after diagnosis of HIV/AIDS in their daily lives. Final considerations: The diagnosis of HIV/AIDS seropositivity in the elderly generates a blend of feelings and fears that lead to food changes, adherence to treatment and the renunciation of daily and social habits, manifested as ways of self-care.
Board games: gerotechnology in nursing care practice
Authors: Olympio Paula Cristina de Andrade Pires , Alvim Neide Aparecida Titonelli
Published: May 2018

ABSTRACT Objective: To create a board game as a form of gerotechnology to promote active and healthy aging. Method: This was a qualitative, convergent care study conducted with 32 older adults. Individual interviews, creativity and sensitivity techniques, group discussions, and participant observations guided by Paulo Freire’s theoretical framework of problematization were used to produce data. The participants and researchers assessed the entire production process at the end of the study, after the gerotechnology was implemented in the groups. French discourse analysis was adopted. Results: Patented under registration no. BR 1020160107725, the game was created as a technological product based on the discussions with older adults, considering the conceptions, knowledge and practices shared by them about aging. Final considerations: The gerotechnology implemented in clinical nursing practices functioned as a playful resource to exercise self-determination and independence among older adults, boosting memory, self-esteem, socialization processes, exchanging experiences and shared learning.
Suicidal ideation and attempt of older women in Northeastern Brazil
Authors: Silva Raimunda Magalhães da , Sousa Girliani Silva de , Vieira Luiza Jane Eyre de Souza , Caldas José Manuel Peixoto , Minayo Maria Cecília de Souza
Published: May 2018

ABSTRACT Objective: to associate the life experiences of older women from the Northeast of Brazil with their suicidal ideation and attempts. Method: Qualitative study with fourteen older adults from Piripiri (PI), Teresina (PI), Fortaleza (CE) and Recife (PE) who were interviewed between November 2013 and July 2014. The organization of data originated the themes: experiences of abuse throughout their lives; marital fractures and social isolation; weakened motherhood. Results: The women were born and lived most of their life in the countryside, amid poverty and social exclusion. They had a history of abuse, physical and sexual violence, perpetrated by intimate partners and/or family members. They experienced social isolation, weakened emotional bonds and several suicide attempts. Final considerations: The life of these older women was affected by traumatic events that enhanced feelings of hopelessness, depressive symptoms and the absence of a plan for the future, making them consider suicide as a way to anticipate death.
Diferenciais de Rendimentos entre Raças e Gêneros, nas Regiões Metropolitanas, por Níveis Ocupacionais: uma análise através do pareamento de Ñopo
Authors: Mariano Francisca Zilania , Costa Edward Martins , Guimarães Daniel Barboza , Sousa Daniel Tomaz de
Published: May 2018

Resumo Este artigo busca analisar o comportamento dos diferenciais de rendimentos entre gênero e raças nas regiões metropolitanas (RMs) e o Distrito Federal, considerando diferentes categorias ocupacionais. Dada a disparidade regional, optou-se por corrigir a renda através do índice de custo de vida desenvolvido por Azzoni e Almeida (2016); para captar o diferencial, os efeitos dotação e discriminação, utilizou-se o método não paramétrico de Ñopo (2008), aplicado aos dados obtidos pela PNAD (2014). Além disso, o método de Oaxaca-Blinder (1973) foi aplicado para o Brasil Metropolitano. Da comparação entre os métodos, constatou-se que Oaxaca superestima os efeitos da decomposição de rendimentos. Em relação ao diferencial, ocupacional e RM, as mulheres, assim como os indivíduos não brancos, têm piores remunerações. Com as ocupações desagregadas e por regiões, os profissionais das ciências e das artes são aqueles que têm as maiores diferenças salariais nas RMs por gênero; por raça, as maiores diferenças são encontradas para o grupo de dirigentes e profissionais das ciências e das artes.
Prevalence of polymorphisms in the ANKK1, DRD2, DRD3 genes and metabolic syndrome in refractory schizophrenia
Authors: Pinto Jeizziani Aparecida Ferreira , Freitas Pedro Henrique Batista de , Nunes Fernanda Daniela Dorneles , Granjeiro Paulo Afonso , Santos Luciana Lara dos , Machado Richardson Miranda
Published: May 2018

ABSTRACT Objective: to estimate the prevalence of TaqIA, -141C and rs6280 polymorphisms of the ANKK1, DRD2 and DRD3 genes and evaluate their association with the occurrence of metabolic syndrome in patients with refractory schizophrenia. Method: cross-sectional study conducted in the Extended Western Region of Minas Gerais, with refractory schizophrenic patients using the antipsychotic clozapine. Sociodemographic, clinical, anthropometric, biochemical and genetic data were collected. Univariate analysis of the data was performed. Results: seventy-two patients participated in the study and the occurrence of Metabolic Syndrome was observed in 47.2% of them. There was no association between Metabolic Syndrome and the studied polymorphisms. There was a statistically significant difference in the low HDL parameter with homozygous genotype for the C allele of the -141C polymorphism of the DRD2 gene. Conclusion: a high prevalence of MS was evidenced. The -141C polymorphism was associated with low HDL. Genetic analysis and identification of metabolic alterations in this group of patients can guide drug treatment and provide a better quality of life.
Good practices in normal childbirth: reliability analysis of an instrument by Cronbach’s Alpha
Authors: Gottems Leila Bernarda Donato , Carvalho Elisabete Mesquita Peres De , Guilhem Dirce , Pires Maria Raquel Gomes Maia
Published: May 2018

ABSTRACT Objectives: to analyze the internal consistency of the evaluation instrument of the adherence to the good practices of childbirth and birth care in the professionals, through Cronbach’s Alpha Coefficient for each of the dimensions and for the total instrument. Method: this is a descriptive and cross-sectional study performed in obstetric centers of eleven public hospitals in the Federal District, with a questionnaire applied to 261 professionals who worked in the delivery care. Results: The study was attended by 261 professionals, 42.5% (111) nurses and 57.5% (150) physicians. The reliability evaluation of the instrument by the Cronbach Alfa resulted in 0.53, 0.78 and 0.76 for dimensions 1, 2 and 3, after debugging that resulted in the exclusion of 11 items. Conclusions: the instrument obtained Cronbach’s alpha of 0.80. There is a need for improvement in the items of dimension 1 that refer to attitudes, knowledge, and practices of the organization of the network of care to gestation, childbirth, and birth. However, it can be applied in the way it is used to evaluate practices based on scientific evidence of childbirth care.
Construção da identidade profissional na Educação Interprofissional em Saúde: percepção de egressos
Authors: Rossit Rosana Aparecida Salvador , Freitas Maria Aparecida de Oliveira , Batista Sylvia Helena Souza da Silva , Batista Nildo Alves
Published: May 2018

Trata-se de estudo de abordagem longitudinal qualitativa, descritivo-analítico e natureza exploratória. Apresenta a percepção de egressos acerca da construção da identidade profissional na perspectiva da Educação Interprofissional, com dados coletados mediante entrevista de aprofundamento com egressos de seis profissões da saúde. Os resultados foram organizados em núcleos direcionadores e tratados com análise de conteúdo. Apresentam-se os resultados referentes ao núcleo Educação Interprofissional e à construção da identidade profissional. Os participantes relataram a trajetória formativa em currículo inovador, interprofissional e interdisciplinar, destacando a oportunidade da prática colaborativa e interprofissional como importantes para o desenvolvimento, construção e reforço da identidade profissional em cada área de formação mediante o reconhecimento das especificidades das outras áreas. A delimitação do próprio campo profissional e de papéis na equipe, bem como a ampliação do olhar profissional, também foram evidenciadas pelos egressos.
ATIVIDADE ANTIMICROBIANA DE MICRORGANISMOS ISOLADOS DE GRÃOS DE KEFIR
Authors: Dias Priscila Alves , Silva Daiani Teixeira , Timm Cláudio Dias
Published: May 2018

Resumo Kefir é o produto da fermentação do leite pelos grãos de kefir. Esses grãos contêm uma mistura simbiótica de bactérias e leveduras imersas em uma matriz composta de polissacarídeos e proteínas. Muitos benefícios à saúde humana têm sido atribuídos ao kefir, incluindo atividade antimicrobiana contra bactérias Gram positivas e Gram negativas. A atividade antimicrobiana de 60 microrganismos isolados de grãos de kefir, frente à Escherichia coli O157:H7, Salmonella enterica subsp. enterica sorotipos Typhimurium e Enteritidis, Staphylococcus aureus e Listeria monocytogenes, foi estudada através do teste do antagonismo. A ação antimicrobiana dos sobrenadantes das bactérias ácido-lácticas que apresentaram atividade no teste do antagonismo foi testada. O experimento foi repetido usando sobrenadantes com pH neutralizado. Salmonella Typhimurium e Enteritidis sobreviveram por 24 horas no kefir em fermentação. E. coli O157:H7, S. aureus e L. monocytogenes foram recuperados até 72 horas após o início da fermentação. Todos os isolados apresentaram atividade antimicrobiana contra pelo menos um dos patógenos usados no teste do antagonismo. Sobrenadantes de 25 isolados apresentaram atividade inibitória e três mantiveram essa atividade com pH neutralizado. As bactérias patogênicas estudadas sobreviveram por tempo superior àquele normalmente utilizado para a fermentação do kefir artesanal, o que caracteriza perigo em potencial para o consumidor quando a matéria-prima não apresentar segurança sanitária. Lactobacillus isolados de grãos de kefir apresentam atividade antimicrobiana contra cepas de E. coli O157:H7, Salmonella sorotipos Typhimurium e Enteritidis, S. aureus e L. monocytogenes além daquela exercida pela diminuição do pH.
Jabuticaba skin extracts: phenolic compounds and antibacterial activity
Authors: Oliveira Flávia Cíntia de , Marques Tamara Rezende , Machado Gustavo Henrique Andrade , Carvalho Thaís Cristina Lima de , Caetano Aline Aparecida , Batista Luis Roberto , Corrêa Angelita Duarte
Published: May 2018

Abstract The phenolic compounds from various extracts of jabuticaba skin powder (JSP) were characterized in this study, and the antibacterial activity assessed. The phenolic compounds were extracted from the JSP using four methods: a) acetone extraction - 1 g JSP: 10 mL 70% acetone, resting for 2 hours; b) aqueous extract - 1 g JSP: 15 mL water, under agitation; c) ethanolic extract - 1 g JSP: 15 mL acidified ethanol, under agitation; and d) methanolic extract - 1 g JSP: 50 mL 50% methanol, under reflux. The antibacterial activity was evaluated by the agar diffusion assay, using Escherichia coli ATCC 11229, Salmonella choleraesuis ATCC 6539, Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 6538 and Listeria monocytogenes ATCC 19117. The ethanolic and methanolic extracts showed the highest levels of phenolic compounds, especially of cyanidin chloride, catechin and epicatechin. The extracts did not inhibit the growth of Escherichia coli and Salmonella choleraesuis, but inhibited 30% of the growth of Pseudomonas aeruginosa with an extract concentration of 250 µg mL-1. Against Staphylococcus aureus and Listeria monocytogenes the highest inhibitory effect observed was 41.8% for the ethanolic extract, followed by 36% inhibition by the methanolic extract, thus revealing the potential of these extracts as possible alternatives for use in the food and/or pharmaceutical industries.
Optimización de polifenoles y aceptabilidad de caramelos de goma con extracto de jengibre (Zingiber officinale R.) y miel con diseño de mezclas
Authors: Rodríguez-Zevallos Antonio , Hayayumi-Valdivia María , Siche Raúl
Published: May 2018

Resumen El objetivo de este trabajo fue optimizar el contenido de polifenoles totales, firmeza, dulzor y aceptabilidad general de caramelos de goma desarrollado con extracto de jengibre y seis proporciones de sacarosa, miel de abeja y glucosa. Para estudiar el efecto de las proporciones se utilizó el Diseño de Mezclas Simplex Lattice. El diseño fue repetido para cuatro niveles de extracto de jengibre: 25%, 41.7%, 58.3% y 75%. Los modelos cuadráticos fueron los que mejor describieron el comportamiento de cada variable estudiada (R2 > 0.95), con los cuales se pudo determinar que las concentraciones de sacarosa, miel y glucosa que optimizan el contenido de polifenoles y aceptabilidad sensorial fueron 27.5%, 27.5% y 10%, respectivamente, con extracto de jengibre al 41.7%.
Reflections on end-of-life dialysis
Authors: Castro Manuel Carlos Martins
Published: May 2018

ABSTRACT The world population is aging and diseases such as diabetes mellitus and systemic arterial hypertension are increasing the risk of patients developing chronic kidney disease, leading to an increase in the prevalence of patients on dialysis. The expansion of health services has made it possible to offer dialysis treatment to an increasing number of patients. At the same time, dialysis survival has increased considerably in the last two decades. Thus, patients on dialysis are becoming more numerous, older and with greater number of comorbidities. Although dialysis maintains hydroelectrolytic and metabolic balance, in several patients this is not associated with an improvement in quality of life. Therefore, despite the high social and financial cost of dialysis, patient recovery may be only partial. In these conditions, it is necessary to evaluate the patient individually in relation to the dialysis treatment. This implies reflections on initiating, maintaining or discontinuing treatment. The multidisciplinary team involved in the care of these patients should be familiar with these aspects in order to approach the patient and his/her relatives in an ethical and humanitarian way. In this study, we discuss dialysis in the final phase of life and present a systematic way to address this dilemma.
IgA Nephropathy in Salvador, Brazil. Clinical and laboratory presentation at diagnosis
Authors: Souza Brenda Navarro de , Tavares Maria Brandão , Soares Maria Fernanda Sanches , Santos Washington Luis Conrado dos
Published: May 2018

ABSTRACT Introduction: IgA nephropathy (IgAN) is the most prevalent primary glomerulopathy in the world, but great variation is reported in different countries. In Brazil, the reported prevalence is high in the Southeastern States and low in Salvador, Bahia State, Brazil. Objectives: This study investigated the clinical and histological patterns of patients with IgAN in Salvador, Brazil. Methods: This is a descriptive study that included all patients with a diagnosis of IgAN performed in native kidney biopsies collected from referral nephrology services of public hospitals in Salvador between 2010 and 2015. Results: Thirty-two cases of IgAN were identified, corresponding to 6% of primary glomerulopathies. There was a slight male predominance (56%) and the median age was 30 [22-40] years. Hematuria was present in 79%, non-nephrotic proteinuria was present in 61%, and hypertension was present in 69% of patients. Segmental sclerosis (S1 lesions) was present in 81% of cases, and chronic tubulo-interstitial lesions (T1 and T2 lesions) were present in 44% of cases. Patients with M1 and T2 MEST-C scores exhibited higher serum urea and creatinine than other patients. Conclusion: The prevalence of IgAN was lower in Salvador than other regions of Brazil. Chronic histological lesions and laboratory markers of severe disease were frequent. M1 and T2 MEST-C scores were correlated with markers of renal dysfunction.
Population density of Tibraca limbativentris on flood irrigated rice and alternative host plants
Authors: Pasini Mauricio Paulo Batistella , Lúcio Alessandro Dal’Col , Cargnelutti Filho Alberto , Ribeiro Ana Lúcia de Paula , Zamberlan João Fernando , Lopes Sidinei José
Published: May 2018

Abstract: The objective of this work were to evaluate the population dynamics of the rice stem bug (Tibraca limbativentris) around and in flood irrigated rice cultivation area, to quantify the insect population flow between crops and host plants, and to determine the effect of the species, the diameter of the plant, and the distance of the host plant from the border on the rice stem bug population. The work was conducted in the 2012/2013 harvest and in the off-season, with sampling of rice, Andropogom bicornis, and Andropogon lateralis plants in six cultivated areas, in order to count the number of insects. The population density of the rice stem bug in irrigated rice and alternative host plants presents spatial and temporal dependence. In the cultivated areas, the population density of the rice stem bug increases according to the evolution of the rice phenological stages, with the highest densities concentrated in regions close to the crop borders. The diameter and species of the host plant affect the rice stem bug population. Andropogon bicornis plants have higher population densities than A. lateralis, and plants with larger diameters show higher population densities of the rice stem bug. In the off-season, the greatest population of the rice stem bug on host plants is concentrated up to 45 m from the crop border, but it can disperse until 150 m.
O INIMIGO NOSSO DE CADA DIA: UMA INTERLOCUÇÃO ENTRE PSICANÁLISE E DIREITO
Authors: Cruz Alexandre Dutra Gomes da , Ferrari Ilka Franco
Published: May 2018

Resumo: O artigo estabelece uma interlocução entre a psicanálise e o direito, tomando como recorte as teorizações freudianas acerca da segregação, pautada pela agressividade dirigida ao próximo e, também, da relação entre direito e violência, buscando articulá-las com a doutrina do direito penal do inimigo, formalizada pelo jurista alemão Gunther Jakobs. Para abordar essa temática e mostrar sua pertinência, os autores discutem as megaoperações policiais realizadas recentemente nas favelas do Rio de Janeiro. Depoimentos recolhidos a partir de uma pesquisa documental permitem afirmar que essa prática vem se revelando não apenas segregacionista, mas francamente intolerante e criminalizadora da pobreza.
The Role of Intercalated Cell Nedd4–2 in BP Regulation, Ion Transport, and Transporter Expression
Authors: Nanami Masayoshi , Pham Truyen D. , Kim Young Hee , Yang Baoli , Sutliff Roy L. , Staub Olivier , Klein Janet D. , Lopez-Cayuqueo Karen I. , Chambrey Regine , Park Annie Y. , Wang Xiaonan , Pech Vladimir , Verlander Jill W. , Wall Susan M.
Published: May 2018

Background
Nedd4–2 is an E3 ubiquitin-protein ligase that associates with transport proteins, causing their ubiquitylation, and then internalization and degradation. Previous research has suggested a correlation between Nedd4–2 and BP. In this study, we explored the effect of intercalated cell (IC) Nedd4–2 gene ablation on IC transporter abundance and function and on BP.

Methods We generated IC Nedd4–2 knockout mice using Cre-lox technology and produced global pendrin/Nedd4–2 null mice by breeding global Nedd4–2 null (Nedd4–2−/−
) mice with global pendrin null (Slc26a4−/−
) mice. Mice ate a diet with 1%–4% NaCl; BP was measured by tail cuff and radiotelemetry. We measured transepithelial transport of Cl− and total CO2 and transepithelial voltage in cortical collecting ducts perfused in vitro. Transporter abundance was detected with immunoblots, immunohistochemistry, and immunogold cytochemistry.

Results IC Nedd4–2 gene ablation markedly increased electroneutral Cl−/HCO3
− exchange in the cortical collecting duct, although benzamil-, thiazide-, and bafilomycin-sensitive ion flux changed very little. IC Nedd4–2 gene ablation did not increase the abundance of type B IC transporters, such as AE4 (Slc4a9), H+-ATPase, barttin, or the Na+-dependent Cl−/HCO3
− exchanger (Slc4a8). However, IC Nedd4–2 gene ablation increased CIC-5 total protein abundance, apical plasma membrane pendrin abundance, and the ratio of pendrin expression on the apical membrane to the cytoplasm. IC Nedd4–2 gene ablation increased BP by approximately 10 mm Hg. Moreover, pendrin gene ablation eliminated the increase in BP observed in global Nedd4–2 knockout mice.

Conclusions IC Nedd4–2 regulates Cl−/HCO3
− exchange in ICs., Nedd4–2 gene ablation increases BP in part through its action in these cells.
Neurogerencia como herramienta en la metamorfosis organizacional en Colombia
Authors: Britto-González Tatiana Simary , Fuentes-Molina Natalia , Torres-Cárdenas Virgilia María
Published: May 2018

Introducción: la presente investigación plantea comprender la neurogerencia como herramienta en la metamorfosis organizacional, revelando las disposiciones cerebrales para asumir los procesos de cambio empresariales en el sector minero de los departamentos de La Guajira y Cesar, en Colombia. Materiales y métodos: se siguió un paradigma pospositivista, con metodología cualitativa, a partir del enfoque del introspeccionismo vivencial, según el tipo de investigación fenológica y algunas adaptaciones desde las ciencias de la conducta humana; se diseñó en cinco momentos que emergieron del constructo teórico de la metamorfosis organizacional. Historia de caso: la muestra intencional estuvo conformada por cuatro personas con rol de mando en las organizaciones. Resultados: se evidenció una metamorfosis funcional por cuanto sus resultados favorecieron la evolución de las organizaciones e impactaron positivamente en la construcción de una nueva cultura, donde el respeto a las diferencias y la conciencia de seguridad en el trabajo permitieron la conservación de la vida. Conclusiones: se evidencia que es necesario realizar innovaciones a partir de la escucha activa de las propuestas de sus integrantes para estar en mejora continua y en armonía con la sustancia humanizada del ser.
DETERMINATION OF MINERALS CONTENT IN LEAVES OF MORINGA OLEIFERA BY NEUTRON ACTIVATION ANALYSIS
Authors: Mulyaningsih Theresia Rina , Yusuf Saeful
Published: May 2018

DETERMINATION  OF  MINERALS  CONTENT  IN  LEAVES  OF  MORINGA  OLEIFERA  BY  NEUTRON ACTIVATION ANALYSIS. Moringa oleifera ( Mo) or kelor is a high-value plant,  distributed in many tropical and subtropical countries. This plant can be used as a vegetable,  cosmetic oil, and medicinal plant. The leaves of Mo is rich in essential minerals needed by the body’s health. Moringa oleifera widely cultivated in various places  in  Indonesia,  easily  obtained  and  inexpensive  so  precious  can  be  used  to  overcome  malnutrition. Research on the macro-micro mineral content in Moringa leaves here is still limited. Mineral composition in leaves include  Mo  leaves varies depending on location grow. The purpose of this research is to determine the mineral content in the  leaves of  the Mo  taken from Indonesia. For the preliminary study samples was taken from Central Java to be compared with the results of studies conducted in several other countries. Leaf samples were collected randomly from sampling area. Mineral content in the samples is determined using  Neutron  Activation  Analysis (NAA). Irradiation was carried out at rabbit system of Multipurpose Reactor G.A Siwabessy on neutron flux ~1013 n.cm-2.dt-1. The results obtained  indicate that Mo is rich in  essential minerals, mainly  Ca, Mg, K, Zn, Fe and Cl. Content in dried leaves include: calcium (3.45 % ), magnesium (0.66 %), potassium (3.35 %), chloride (0,25%), iron (147.20 mg/kg), sodium ( 152.52 mg/kg ), zinc (35.71 mg/kg), and manganese (102.10 mg/kg). Mo also contains other minerals such as chromium (4.76 mg/kg), bromine (4.82 mg/kg), cobalt (0.16 mg/kg), and aluminium (150.40 mg/kg) in addition to other element. Compared with the results of existing studies, it shows that mineral composition in Mo leaves varies depending on the location where the plant is grown.