bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Anthropogenic Emission Reduction during COVID-19 on Air Quality in Nanjing, China
Authors: Yao Zehui , Wang Yong , Qiu Xinfa , Song Fanling
Published: March 2023

To avoid the spread of COVID-19, China has implemented strict lockdown policies and control measures, resulting in a dramatic decrease in air pollution and improved air quality. In this study, the air quality model WRF-Chem and the latest MEIC2019 and MEIC2020 anthropogenic emission inventories were used to simulate the air quality during the COVID-19 lockdown in 2020 and the same period in 2019. By designing different emission scenarios, this study explored the impact of the COVID-19 lockdown on the concentration of air pollutants emitted by different sectors (industrial sector and transportation sector) in Nanjing for the first time. The results indicate that influenced by the COVID-19 lockdown policies, compared with the same period in 2019, the concentrations of PM2.5, PM10, and NO2 in Nanjing decreased by 15%, 17.1%, and 20.3%, respectively, while the concentration of O3 increased by 45.1% in comparison; the concentrations of PM2.5, PM10 and NO2 emitted by industrial sector decreased by 30.7%, 30.8% and 14.0% respectively; the concentrations of PM2.5, PM10 and NO2 emitted by transportation sector decreased by 15.6%, 15.7% and 26.2% respectively. The COVID-19 lockdown has a greater impact on the concentrations of PM2.5 and PM10 emitted by the industrial sector, while the impact on air pollutants emitted by the transportation sector is more reflected in the concentration of NO2. This study provides some theoretical basis for the treatment of air pollutants in different departments in Nanjing.
Fuzzy Algebras of Concepts
Authors: Ojeda-Hernández Manuel , López-Rodríguez Domingo , Cordero Pablo
Published: March 2023

Preconcepts are basic units of knowledge that form the basis of formal concepts in formal concept analysis (FCA). This paper investigates the relations among different kinds of preconcepts, such as protoconcepts, meet and join-semiconcepts and formal concepts. The first contribution of this paper, is to present a fuzzy powerset lattice gradation, that coincides with the preconcept lattice at its 1-cut. The second and more significant contribution, is to introduce a preconcept algebra gradation that yields different algebras for protoconcepts, semiconcepts, and concepts at different cuts. This result reveals new insights into the structure and properties of the different categories of preconcepts.
Application of a Magnetic Platform in α6 Integrin-Positive iPSC-TM Purification
Authors: Feng Pengchao , Wang Wenyan , Xu Wenhua , Cao Qilong , Zhu Wei
Published: March 2023

The emergence of induced pluripotent stem cell (iPSC) technology has provided a new approach to regenerating decellularized trabecular meshwork (TM) in glaucoma. We have previously generated iPSC-derived TM (iPSC-TM) using a medium conditioned by TM cells and verified its function in tissue regeneration. Because of the heterogeneity of iPSCs and the isolated TM cells, iPSC-TM cells appear to be heterogeneous, which impedes our understanding of how the decellularized TM may be regenerated. Herein, we developed a protocol based on a magnetic-activated cell sorting (MACS) system or an immunopanning (IP) method for sorting integrin subunit alpha 6 (ITGA6)-positive iPSC-TM, an example of the iPSC-TM subpopulation. We first analyzed the purification efficiency of these two approaches by flow cytometry. In addition, we also determined cell viability by analyzing the morphologies of the purified cells. To conclude, the MACS-based purification could yield a higher ratio of ITGA6-positive iPSC-TM and maintain a relatively higher cell viability than the IP-based method, allowing for the preparation of any iPSC-TM subpopulation of interest and facilitating a better understanding of the regenerative mechanism of iPSC-based therapy.
An Organismal Perspective on the Warburg Effect and Models for Proliferation Studies
Authors: Blackstone Neil W. , El Rahmany Weam S.
Published: March 2023

Interest in the physiology of proliferation has been generated by human proliferative diseases, i.e., cancers. A vast literature exists on the Warburg effect, which is characterized by aerobic glycolysis, diminished oxygen uptake, and lactate secretion. While these features could be rationalized via the production of biosynthetic precursors, lactate secretion does not fit this paradigm, as it wastes precursors. Forming lactate from pyruvate allows for reoxidizing cytosolic NADH, which is crucial for continued glycolysis and may allow for maintaining large pools of metabolic intermediates. Alternatively, lactate production may not be adaptive, but rather reflect metabolic constraints. A broader sampling of the physiology of proliferation, particularly in organisms that could reoxidize NADH using other pathways, may be necessary to understand the Warburg effect. The best-studied metazoans (e.g., worms, flies, and mice) may not be suitable, as they undergo limited proliferation before initiating meiosis. In contrast, some metazoans (e.g., colonial marine hydrozoans) exhibit a stage in the life cycle (the polyp stage) that only undergoes mitotic proliferation and never carries out meiosis (the medusa stage performs this). Such organisms are prime candidates for general studies of proliferation in multicellular organisms and could at least complement the short-generation models of modern biology.
Diminishing the Pathogenesis of the Food-Borne Pathogen Serratia marcescens by Low Doses of Sodium Citrate
Authors: Khayat Maan T. , Elbaramawi Samar S. , Nazeih Shaimaa I. , Safo Martin K. , Khafagy El-Sayed , Ali Mohamed A. M. , Abbas Hisham A. , Hegazy Wael A. H. , Seleem Noura M.
Published: March 2023

Protecting food from bacterial contamination is crucial for ensuring its safety and avoiding foodborne illness. Serratia marcescens is one of the food bacterial contaminants that can form biofilms and pigments that spoil the food product and could cause infections and illness to the consumer. Food preservation is essential to diminish such bacterial contaminants or at least reduce their pathogenesis; however, it should not affect food odor, taste, and consistency and must be safe. Sodium citrate is a well-known safe food additive and the current study aims to evaluate its anti-virulence and anti-biofilm activity at low concentrations against S. marcescens. The anti-virulence and antibiofilm activities of sodium citrate were evaluated phenotypically and genotypically. The results showed the significant effect of sodium citrate on decreasing the biofilm formation and other virulence factors, such as motility and the production of prodigiosin, protease, and hemolysins. This could be owed to its downregulating effect on the virulence-encoding genes. An in vivo investigation was conducted on mice and the histopathological examination of isolated tissues from the liver and kidney of mice confirmed the anti-virulence activity of sodium citrate. In addition, an in silico docking study was conducted to evaluate the sodium citrate binding ability to S. marcescens quorum sensing (QS) receptors that regulates its virulence. Sodium citrate showed a marked virtual ability to compete on QS proteins, which could explain sodium citrate’s anti-virulence effect. In conclusion, sodium citrate is a safe food additive and can be used at low concentrations to prevent contamination and biofilm formation by S. marcescens and other bacteria.
Molecular Mechanisms and Clinical Phenotypes of GJB2 Missense Variants
Authors: Mao Lu , Wang Yueqiang , An Lei , Zeng Beiping , Wang Yanyan , Frishman Dmitrij , Liu Mengli , Chen Yanyu , Tang Wenxue , Xu Hongen
Published: March 2023

The GJB2 gene is the most common gene responsible for hearing loss (HL) worldwide, and missense variants are the most abundant type. GJB2 pathogenic missense variants cause nonsyndromic HL (autosomal recessive and dominant) and syndromic HL combined with skin diseases. However, the mechanism by which these different missense variants cause the different phenotypes is unknown. Over 2/3 of the GJB2 missense variants have yet to be functionally studied and are currently classified as variants of uncertain significance (VUS). Based on these functionally determined missense variants, we reviewed the clinical phenotypes and investigated the molecular mechanisms that affected hemichannel and gap junction functions, including connexin biosynthesis, trafficking, oligomerization into connexons, permeability, and interactions between other coexpressed connexins. We predict that all possible GJB2 missense variants will be described in the future by deep mutational scanning technology and optimizing computational models. Therefore, the mechanisms by which different missense variants cause different phenotypes will be fully elucidated.
Genome and Genetic Engineering of the House Cricket (Acheta domesticus): A Resource for Sustainable Agriculture
Authors: Dossey Aaron T. , Oppert Brenda , Chu Fu-Chyun , Lorenzen Marcé D. , Scheffler Brian , Simpson Sheron , Koren Sergey , Johnston J. Spencer , Kataoka Kosuke , Ide Keigo
Published: March 2023

Background: The house cricket, Acheta domesticus, is one of the most farmed insects worldwide and the foundation of an emerging industry using insects as a sustainable food source. Edible insects present a promising alternative for protein production amid a plethora of reports on climate change and biodiversity loss largely driven by agriculture. As with other crops, genetic resources are needed to improve crickets for food and other applications. Methods: We present the first high quality annotated genome assembly of A. domesticus from long read data and scaffolded to chromosome level, providing information needed for genetic manipulation. Results: Gene groups related to immunity were annotated and will be useful for improving value to insect farmers. Metagenome scaffolds in the A. domesticus assembly, including Invertebrate Iridescent Virus 6 (IIV6), were submitted as host-associated sequences. We demonstrate both CRISPR/Cas9-mediated knock-in and knock-out of A. domesticus and discuss implications for the food, pharmaceutical, and other industries. RNAi was demonstrated to disrupt the function of the vermilion eye-color gene producing a useful white-eye biomarker phenotype. Conclusions: We are utilizing these data to develop technologies for downstream commercial applications, including more nutritious and disease-resistant crickets, as well as lines producing valuable bioproducts, such as vaccines and antibiotics.
Effects of GHR Deficiency and Juvenile Hypoglycemia on Immune Cells of a Porcine Model for Laron Syndrome
Authors: Schilloks Marie-Christin , Giese Isabella-Maria , Hinrichs Arne , Korbonits Lucia , Hauck Stefanie M. , Wolf Eckhard , Deeg Cornelia A.
Published: March 2023

Laron syndrome (LS) is a rare genetic disorder characterized by low levels of insulin-like growth factor 1 (IGF1) and high levels of growth hormone (GH) due to mutations in the growth hormone receptor gene (GHR). A GHR-knockout (GHR-KO) pig was developed as a model for LS, which displays many of the same features as humans with LS-like transient juvenile hypoglycemia. This study aimed to investigate the effects of impaired GHR signaling on immune functions and immunometabolism in GHR-KO pigs. GHR are located on various cell types of the immune system. Therefore, we investigated lymphocyte subsets, proliferative and respiratory capacity of peripheral blood mononuclear cells (PBMCs), proteome profiles of CD4− and CD4+ lymphocytes and IFN-α serum levels between wild-type (WT) controls and GHR-KO pigs, which revealed significant differences in the relative proportion of the CD4+CD8α− subpopulation and in IFN-α levels. We detected no significant difference in the respiratory capacity and the capacity for polyclonal stimulation in PBMCs between the two groups. But proteome analysis of CD4+ and CD4− lymphocyte populations revealed multiple significant protein abundance differences between GHR-KO and WT pigs, involving pathways related to amino acid metabolism, beta-oxidation of fatty acids, insulin secretion signaling, and oxidative phosphorylation. This study highlights the potential use of GHR-KO pigs as a model for studying the effects of impaired GHR signaling on immune functions.
HSP70 Family in Cancer: Signaling Mechanisms and Therapeutic Advances
Authors: Zhao Kejia , Zhou Guanyu , Liu Yu , Zhang Jian , Chen Yaohui , Liu Lunxu , Zhang Gao
Published: March 2023

The 70 kDa heat shock proteins (HSP70s) are a group of highly conserved and inducible heat shock proteins. One of the main functions of HSP70s is to act as molecular chaperones that are involved in a large variety of cellular protein folding and remodeling processes. HSP70s are found to be over-expressed and may serve as prognostic markers in many types of cancers. HSP70s are also involved in most of the molecular processes of cancer hallmarks as well as the growth and survival of cancer cells. In fact, many effects of HSP70s on cancer cells are not only related to their chaperone activities but rather to their roles in regulating cancer cell signaling. Therefore, a number of drugs directly or indirectly targeting HSP70s, and their co-chaperones have been developed aiming to treat cancer. In this review, we summarized HSP70-related cancer signaling pathways and corresponding key proteins regulated by the family of HSP70s. In addition, we also summarized various treatment approaches and progress of anti-tumor therapy based on targeting HSP70 family proteins.
Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules
Authors: Theyagarajan K. , Kim Young-Joon
Published: March 2023

Electrochemical biosensors are superior technologies that are used to detect or sense biologically and environmentally significant analytes in a laboratory environment, or even in the form of portable handheld or wearable electronics. Recently, imprinted and implantable biosensors are emerging as point-of-care devices, which monitor the target analytes in a continuous environment and alert the intended users to anomalies. The stability and performance of the developed biosensor depend on the nature and properties of the electrode material or the platform on which the biosensor is constructed. Therefore, the biosensor platform plays an integral role in the effectiveness of the developed biosensor. Enormous effort has been dedicated to the rational design of the electrode material and to fabrication strategies for improving the performance of developed biosensors. Every year, in the search for multifarious electrode materials, thousands of new biosensor platforms are reported. Moreover, in order to construct an effectual biosensor, the researcher should familiarize themself with the sensible strategies behind electrode fabrication. Thus, we intend to shed light on various strategies and methodologies utilized in the design and fabrication of electrochemical biosensors that facilitate sensitive and selective detection of significant analytes. Furthermore, this review highlights the advantages of various electrode materials and the correlation between immobilized biomolecules and modified surfaces.
Electroacupuncture at Fengchi(GB20) and Yanglingquan(GB34) Ameliorates Paralgesia through Microglia-Mediated Neuroinflammation in a Rat Model of Migraine
Authors: Zhou Min , Pang Fang , Liao Dongmei , He Xinlu , Yang Yunhao , Tang Chenglin
Published: March 2023

Background: Multiple studies have suggested that paralgesia (hyperalgesia and cutaneous allodynia) in migraine reflects the activation and sensitisation of the trigeminovascular system (TGVS). In particular, it reflects the second-order and higher nerve centre sensitisation, which is caused and maintained by neuroinflammation. Microglia activation leads to the release of proinflammatory cytokines involved in inflammatory responses. Accumulating evidence indicates that electroacupuncture (EA) is effective in ameliorating paralgesia, but the underlying mechanisms of EA in migraine attacks caused by microglia and microglia-mediated inflammatory responses are still unclear. The purpose of this study was to explore whether EA could ameliorate the dysregulation of pain sensation by suppressing microglial activation and the resulting neuroinflammatory response, and to evaluate whether this response was regulated by Toll-like receptor 4 (TLR4)/nuclear factor-kappa B(NF-κB) in the trigeminal nucleus caudalis (TNC) in a rat model of migraine. Methods: Repeated Inflammatory Soup (IS) was infused into the dura for seven sessions to establish a recurrent migraine-like rat model, and EA treatment was administered at Fengchi (GB20) and Yanglingquan (GB34) after daily IS infusion. Facial mechanical withdrawal thresholds were measured to evaluate the change in pain perception, and plasma samples and the TNC tissues of rats were collected to examine the changes in calcitonin gene-related peptide (CGRP), the Ibal-1-labelled microglial activation, and the resulting inflammatory response, including interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and their regulatory molecules TLR4/NF-κB, via enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (RT-PCR), immunohistochemistry (IHC) and Western blot analysis. Results: Repeated IS injections into the dura induced facial mechanical paralgesia, which is the manifestation of migraine attacks, and increased the expression of CGRP, Ibal-1, microglial mediated inflammatory cytokines (IL-1β, TNF-α, IL-6), and regulatory molecules TLR4/NF-κB. EA at GB20/34 significantly attenuated repetitive IS-induced pain hypersensitivity. This effect was consistent with decreased levels of CGRP and inflammatory cytokines in the plasma and the TNC via the inhibition of microglia activation, and this response may be regulated by TLR4/NF-κB. Conclusions: EA ameliorated paralgesia in repetitive IS-induced migraine-like rats, which was mainly mediated by a reduction in microglial activation and microglial-mediated inflammatory responses that could be regulated by TLR4/NF-κB.
Organizational Challenges in Healthcare Services Providers for Individuals with Autism Spectrum Disorder (ASD) Considering Personnel Turnover Rate
Authors: Samadi Sayyed Ali , Biçak Cemal A. , Osman Nigar , Abdalla Barez
Published: March 2023

The organizational strategy and environment of the healthcare systems influence the turnover rate among healthcare provider personnel. These critical factors have received scant attention in the literature and particularly in the healthcare systems of the Kurdistan Region of Iraq (KRI) as one low-income country with a lack of infrastructural health provision foundations. In this cross-sectional study with qualitative synthesis, the turnover rate of a population consisting of 115 (85 “74%” rehabilitation and training personnel) members of a daycare center for individuals with autism spectrum disorder (ASD) during eight years in the city of Erbil was considered. The aim was to understand the organizational strategy and its impacts on the personnel turnover rate, particularly among healthcare providers. The analysis was conducted utilizing collective data over an eight-year period of service provisions. A qualitative methodological approach was adopted to understand the different aspects of turnover among the staff. The finding indicated a general turnover rate of 87% for the entire personnel and 72% for the rehabilitation and training service providers. This rate imposes considerable costs on the system. Interview analysis also yields supporting evidence for the desire of the staff to change jobs and the lack of understanding of the turnover among system authoritarians resulting in unpredictable changes and staff displacement. Further investigations are needed to understand different contributing factors to imposed or desired turnover rates among healthcare providers in KRI. The turnover over the years has imposed different challenges for the center where the data was collected and imposes unwanted negative impacts on healthcare organizations.
New Insight into Selective Serotonin Receptor Agonists in the Central Nervous System, Studied with WAY163909 in Obese and Diabetic Wistar Rats
Authors: Bogomilov Ivaylo , Boyadjieva Nadka , Nikolov Rumen
Published: March 2023

Background and aims: We investigated the effect of WAY-163909, a novel 5-hydroxytryptamine selective 2C receptor agonist on body weight, blood glucose levels, and insulin resistance in obese and diabetic Wistar rats. Materials and methods: We used twenty male Wistar rats with obesity and obesity-induced diabetes and twenty healthy Wistar rats as a control group. Each of these groups was separated into two subgroups: one with a daily intraperitoneal application of WAY-163909 (1 mg/kg) and one without. During the study, body weight, blood glucose levels, and immunoreactive insulin were tracked. Results: A reduction of 5.5% (p < 0.05) in body weight was registered in the rat group with diabetes and obesity and 2.56% in the control group with a daily application of WAY-163909 (1 mg/kg) at the end of the study. Decreases of 35.4% in blood glucose levels at week four in the diabetic and obese rat group with a daily application of WAY-163909 (1 mg/kg) were registered. A reduction of insulin levels of 4.1% (p < 0.05) in the diabetic and obese rats group using WAY-163909 was also observed. Conclusion: In our study, using WAY-163909 (1 mg/kg) led to a reduction of blood glucose levels, immunoreactive insulin, and body weight.
Cortical Thickness of the Orbitofrontal Cortex in Patients with Alcohol Use Disorder
Authors: Atmaca Murad , Tabara Muhammed Fatih , Koc Mustafa , Gurok Mehmet Gurkan , Baykara Sema , Korkmaz Sevda , Mermi Osman
Published: March 2023

Aims: In the present study, it was hypothesised that compared to healthy control subjects, significant differences in the cortical thickness of the orbitofrontal cortex (OFC) region of the brain, which is relevant to both impulsivity and decision making, would be identified. Methods: The subject groups included in the study were composed of 15 individuals who met the criteria for alcohol use disorder, according to the Diagnostic and Statistical Manual of Mental Disorders Fifth Edition (DSM 5) diagnostic criteria based on the Structured Clinical Interview for DSM 5 (SCID), and were admitted to the Firat University School of Medicine Department of Psychiatry or were hospitalised, and 17 healthy control comparisons were made. The volumes of and cortical thickness of the OFC were measured in the subjects. Results: It was found that patients with alcohol use disorder had reduced volumes of the OFC bilaterally and a thinner cortical thickness of the same region bilaterally compared to those of the healthy control comparisons. Conclusions: Consequently, it is suggested that the OFC region of the brain appears to be statistically significantly smaller in patients with alcohol use disorder, both in terms of cortical thickness and volume, compared to healthy controls. Future research should focus on the status of these relationships longitudinally and should assess the causality of the association with the treatment response.
Improving Effects of Peripheral Nerve Decompression Microsurgery of Lower Limbs in Patients with Diabetic Peripheral Neuropathy
Authors: Ma Fukai , Wang Guangyu , Wu Yiwei , Xie Bingran , Zhang Wenchuan
Published: March 2023

Background: Peripheral nerve decompression microsurgery can relieve nerve entrapment and improve the symptoms of DPN. However, postoperative tissue adhesion will produce new pressure on the nerves, affecting the surgical efficacy. In this study, a nerve conduit was used in the peripheral nerve decompression microsurgery to prevent postoperative adhesions, and the role of the nerve conduit in surgical nerve decompression was explored. Methods: A total of 69 patients with DPN were recruited and randomly divided into three groups: the nerve conduit group, conventional surgery group, and control group. Two weeks before surgery and 6 months after surgery, patients in each group were clinically tested using the visual analog scale (VAS) score, neurophysiological test, Toronto clinical scoring system (TCSS) score, and two-point discrimination (2-PD) test. Results: The patients’ symptoms in the nerve conduit group were relieved to varying degrees, and the relief rate reached 90.9%; the treatment efficacy was higher than that in the other groups. The postoperative nerve conduction velocity (NCV) in the two surgical groups was significantly higher than that before the surgery, and the difference between the nerve conduit group and the conventional surgery group was statistically significant (p < 0.05). For the 2-PD test, there was a statistically significant difference between the two surgical groups (p < 0.05). The TCSS score in the two surgical groups was significantly higher than that in the control group (p < 0.01). There was a significant difference in the TCSS scores between the nerve conduit group and the conventional surgery group (p < 0.05). Conclusions: The nerve conduit could further improve the efficacy of peripheral nerve decompression microsurgery in the treatment of DPN.
Strategic Processing of Gender Stereotypes in Sentence Comprehension: An ERP Study
Authors: Du Yanan , Zhang Yaxu
Published: March 2023

Gender stereotypes are often involved in language comprehension. This study investigated whether and to what extent their processing is under strategic control, by examining both proportion and order effects related to gender stereotypes for role nouns. We manipulated stereotypical gender consistencies, as in “Li’s daughter/son was a nurse…”, the relative proportions of gender-consistent and gender-inconsistent sentences (80%:20% and 50%:50% for high-proportion and equal-proportion sessions, respectively), and a between-participant factor of session order (high-proportion sessions preceding equal-proportion sessions and a reversed order for the high–equal and equal–high groups, respectively). Linear mixed-effect models revealed a larger N400 and a larger late negativity for stereotypically inconsistent compared to consistent sentences for the high–equal group only. These results indicate that even if sentence contexts have already determined the gender of target role characters, gender stereotypes for role nouns are still activated when the first half of the experiment facilitates their activation. The analyses of trial-by-trial dynamics showed that the N400 effects gradually decreased throughout equal-proportion sessions for the equal–high group. Our findings suggest that the processing of gender stereotypes can be under strategic control. In addition, readers may develop other strategies based on sentence contexts, when the processing strategy based on cue validity is not available.
Early Emotional Experiences and Prosocial Behavior among Chinese Adolescents: The Roles of Psychological Suzhi and Subjective Socioeconomic Status
Authors: Liu Xiaoyi , Cao Gege , Zhang Li , Chen Yang , Liu Sige , Shi Yan , Liu Yunpeng , Li Yulin , Yin Huazhan
Published: March 2023

Prosocial behavior plays a vital role in adolescents’ well-being and social functioning, with the recall of early emotional experiences being a major influence. Positive experiences such as early memories of warmth and safeness (EMWS) contribute to prosocial interpersonal characteristics, whereas adverse experiences such as child psychological abuse and neglect (CPAN) lead to social withdrawal or behavioral problems. The direct effects of EMWS and CPAN on prosocial behavior were investigated in this study, along with the mediation effect of psychological suzhi and the moderation effect of subjective socioeconomic status (SSS). A sample of 948 adolescents (Mage = 14.05 years, SD = 1.68 years; 43.6% females) was randomly recruited to complete self-report questionnaires. Correlation results indicated that EMWS promoted prosocial behavior, whereas CPAN was negatively associated with prosocial behavior. Path analyses confirmed the mediating role of psychological suzhi on the effects of EMWS and CPAN on prosocial behavior. SSS was shown to moderate the effects of EMWS on prosocial behavior and CPAN on psychological suzhi. Compared to lower SSS, higher SSS would reinforce the positive impact of EMWS on prosocial behavior and exacerbate the negative impact of CPAN on psychological suzhi. The current study provided new insight into understanding the underlying mechanisms of prosocial behavior from the perspective of early emotional experiences.
Evaluation of the Impact of Input-Data Resolution on Building-Energy Simulation Accuracy and Computational Load—A Case Study of a Low-Rise Office Building
Authors: Kong Dezhou , Yang Yimin , Sa Xingning , Wei Xuanyue , Zheng Huoyu , Shi Jiwei , Wu Hongyi , Zhang Zhiang
Published: March 2023

Building-energy consumption is the primary aim of urban energy consumption, which can aid in optimization of building operation and management techniques, creating sustainable building and built environments. However, modellers’ understanding of the relationship between building-energy modelling (BEM) accuracy and computational load is still qualitative and deprived of accurate quantitative study. Based on a bottom-up engineering methodology, this study aims to quantitatively explore the effects of building-model input data with different resolution accuracies on energy simulation results, including evaluation of computational load. According to the actual parameters of the case-study building, 108 models with varying input resolution levels were developed to estimate hourly energy usage and annual mean ambient temperature. The results demonstrated that with input parameters at low resolution levels, geometric parameters such as exterior windows, interior windows, and shading exhibited significantly lower computational loads, resulting in reduced errors in the final simulation performance, whereas the occupancy schedule, thermal zoning, and HVAC configuration parameters exhibited significant declines in simulation performance and accuracy. This study presents a methodology applicable to the majority of low-rise, rectangular office structures. Future work would concentrate on carrying out comparison tests for different building forms and types while gradually improving the automation of the process to enable use of the appropriate accuracy level in assessing the crucial issue of energy-modelling input.
Aesthetically Appealing Building Integrated Photovoltaic Systems for Net-Zero Energy Buildings. Current Status, Challenges, and Future Developments—A Review
Authors: Basher Mohammad Khairul , Nur-E-Alam Mohammad , Rahman Md Momtazur , Alameh Kamal , Hinckley Steven
Published: March 2023

With the sharp increase in global energy demand, industrial and residential buildings are responsible for around 40% of the energy consumed with most of this energy portion being generated by non-renewable sources, which significantly contribute to global warming and environmental hazards. The net-zero energy building (NZEB) concept attempts to solve the global warming issue, whereby a building will produce, on-site, its required energy demand throughout the year from renewable energy sources. This can be achieved by integrating photovoltaic (PV) building materials, called building-integrated photovoltaic (BIPV) modules, throughout the building skin, which simultaneously act as construction materials and energy generators. Currently, architects and builders are inclined to design a building using BIPV modules due to the limited colors available, namely, black or blue, which result in a monotonous building appearance. Therefore, there is an increasing demand/need to develop modern, aesthetically pleasing BIPV green energy products for the use of architects and the construction industry. This review article presents the current stage and future goal of advanced building integrated photovoltaic systems, focusing on the aesthetically appealing BIPV systems, and their applications towards overcoming global challenges and stepping forward to achieve a sustainable green energy building environment. Additionally, we present the summary and outlook for the future development of aesthetically appealing building integrated photovoltaic systems.
Drivers of, and Barriers to, the Adoption of Mixed Reality in the Construction Industry of Developing Countries
Authors: Maqsoom Ahsen , Zulqarnain Muhammad , Irfan Muhammad , Ullah Fahim , Alqahtani Fahad , Khan Khurram
Published: March 2023

Mixed Reality (MR) that combines elements of both augmented reality (AR) and virtual reality (VR) has great potential for use in the construction industry. However, its usage in construction projects in developing countries has not been widely researched. This study aims to examine the major drivers of, and barriers to, the adoption of MR technologies (MRTs) in the construction sector of developing countries. A mixed methodology that included both qualitative and quantitative data analysis was used. The literature review revealed 37 barriers to, and 41 drivers of, MR adoption. A questionnaire was then distributed to 220 randomly selected respondents from the pertinent construction industry, representing all major stakeholders. The relative importance index (RII) was used to rank the barriers and drivers in terms of significance. The results showed that the primary barriers to MR adoption are the high cost of initial investment, public perception of the technology being immature, limited demand, and difficulty accessing relevant experts’ knowledge. The key drivers of MR adoption include improved project knowledge, reduced overall project costs, low-cost and realistic training scenarios, reduced damage and development costs, and enhanced user experience. These findings provide insights into the major barriers and drivers of MR in the construction sector of developing countries and will help pertinent companies to focus their research and development (R&D) efforts on overcoming these barriers and promote their adoption to move towards the much sought-after construction automation and digitalization.
Update on PET Radiopharmaceuticals for Imaging Hepatocellular Carcinoma
Authors: Nyakale Nozipho , Filippi Luca , Aldous Colleen , Sathekge Mike
Published: March 2023

Numerous positron emission tomography (PET) targets for detection and staging of hepatocellular cancer have been developed in recent years. Hepatocellular carcinomas (HCCs) are clinically and pathologically heterogeneous tumours with a high tendency to be aggressive and unresponsive to chemotherapy. Early detection is essential, and the need for an adequate imaging biomarker, which can overcome some of the limitations of conventional radiological imaging, is persistent. Flourine-18 (18F) flourodeoxyglucose (FDG), the most widely used PET radiopharmaceutical, has proven disappointing as a possible staple in the evaluation of HCC. This disappointment had led to experimentation with carious radiotracers, such as the choline derivatives, acetate, and prostate-specific membrane antigen, which appear to complement and/or enhance the role of FDG. In this study, we look at the various PET radiopharmaceuticals that have been used for imaging HCC and the particular pathways that they target in HCC and liver cancers.
Association between Posttreatment Serum Platelet-to-Lymphocyte Ratio and Distant Metastases in Patients with Hepatocellular Carcinoma Receiving Curative Radiation Therapy
Authors: Lee Dong Soo , Kim Chang Wook , Kim Hee Yeon , Ku Young-Mi , Won Yoo Dong , Lee Su-Lim , Sun Der Sheng
Published: March 2023

Background: We sought to investigate whether serum immune and inflammatory parameters can help to predict distant metastasis (DM) in patients with unresectable hepatocellular carcinoma (HCC) undergoing curative radiation therapy (RT). Methods: A total of 76 RT courses were analyzed. The following variables were included in the analysis: systemic inflammation index, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio (PLR), prognostic nutritional index (PNI), absolute lymphocyte count, lymphocyte-to-monocyte ratio, albumin, albumin-to-alkaline phosphatase ratio, RT-related parameters, and levels of total protein, hemoglobin, α-fetoprotein, and PIVKA-II. Distant control (DC) and overall survival (OS) rates were calculated and compared. Results: The mean age was 61.4 years, and most patients were men (n = 62, 81.6%). The median RT fraction number and fractional doses were 12 (range, 4–30) and 5 (range, 2–12) Gy, respectively. With a median follow-up of 12 (range, 3.1–56.7) months, the 1-year DC and OS rates were 64.4% and 55.2%, respectively. The development of DM significantly deteriorated OS (p = 0.013). In the multivariate analysis, significant independent prognostic indicators for DC and OS rates were the highest posttreatment PLR (≤235.7 vs. >235.7, p = 0.006) and the lowest posttreatment PNI (≤25.4 vs. >25.4, p < 0.001), respectively. Conclusions: Posttreatment serum PLR might be helpfully used as a predictive biomarker of DM in unresectable HCC patients undergoing RT. Future research is necessary to confirm our findings.
Parathyroid Hormone-Related Protein/Parathyroid Hormone Receptor 1 Signaling in Cancer and Metastasis
Authors: Zhao Yawei , Su Shang , Li Xiaohong
Published: March 2023

PTHrP exerts its effects by binding to its receptor, PTH1R, a G protein-coupled receptor (GPCR), activating the downstream cAMP signaling pathway. As an autocrine, paracrine, or intracrine factor, PTHrP has been found to stimulate cancer cell proliferation, inhibit apoptosis, and promote tumor-induced osteolysis of bone. Despite these findings, attempts to develop PTHrP and PTH1R as drug targets have not produced successful results in the clinic. Nevertheless, the efficacy of blocking PTHrP and PTH1R has been shown in various types of cancer, suggesting its potential for therapeutic applications. In light of these conflicting data, we conducted a comprehensive review of the studies of PTHrP/PTH1R in cancer progression and metastasis and highlighted the strengths and limitations of targeting PTHrP or PTH1R in cancer therapy. This review also offers our perspectives for future research in this field.
Is It Time to Cross the Pillars of Evidence in Favor of Segmentectomies in Early-Stage Non-Small Cell Lung Cancer?
Authors: Bertolaccini Luca , Spaggiari Lorenzo
Published: March 2023

In the debate on lobectomy versus segmentectomy for the treatment of early-stage non-small cell lung cancer (NSCLC), currently, we have reached two pillars of knowledge, like Jachim and Boaz, which have encompassed the actual boundary of the literature published up until now [...]
Efficacy and Safety of Bruton Tyrosine Kinase Inhibitor Monotherapy Compared with Combination Therapy for Chronic Lymphocytic Leukemia and Small Lymphocytic Lymphoma: A Systematic Review and Meta-Analysis
Authors: Nguyen Thi Thuy , Nhu Nguyen Thanh , Tran Van Khoi , Nguyen Tran Thuc Huan , Lin Chiou-Feng
Published: March 2023

The effectiveness and safety of combination treatments such as chemoimmunotherapies in chronic lymphocytic leukemia and small lymphocytic lymphoma (CLL/SLL) remain controversial. Bruton tyrosine kinase inhibitors (BTKis) are an effective therapy for CLL/SLL patients. This meta-analysis aimed to compare the efficacy and safety of BTKis versus combination therapy in CLL/SLL patients. We searched the PubMed, Cochrane, Medline, and Embase databases through February 2023 for relevant randomized controlled trials (RCTs). Four RCTs (including 1510 patients) were found and met the inclusion criteria. Progression-free survival (PFS) was significantly improved with BTKis when compared to the combination therapy (hazard ratio (HR), 0.30; 95% confidence interval (CI), 0.22–0.40), while a pooled analysis of overall survival did not favor single-agent BTKis over the combination therapy (HR, 0.87; 95% CI, 0.67–1.15). We observed consistent benefits for PFS among patients with high-risk disease characteristics. Although there was no difference in complete response between the two arms (risk ratio (RR), 0.54; 95% CI, 0.20–1.46), BTKi use was related to a better overall response rate (RR, 1.10; 95% CI, 1.04–1.16). The risk of grade ≥3 adverse events (AEs) was comparable between the two arms (RR, 0.82; 95% CI, 0.55–1.23). However, the risk of grade ≥3 AEs was significantly lower in the second-generation BTKi group than in the combination therapy group (RR, 0.73; 95% CI, 0.54–0.98). Overall, BTKis have superior efficacy compared to the combination regimens in patients with untreated or treated CLL/SLL without excess toxicity. Further studies are needed to confirm these results and determine the optimal therapy for managing patients with CLL/SLL.
Dynamic EPR Studies of the Formation of Catalytically Active Centres in Multicomponent Hydrogenation Systems
Authors: Titova Yuliya Yu.
Published: March 2023

The formation of catalytically active nano-sized cobalt-containing structures in multicomponent hydrogenation systems based on Co(acac)2 complex and various cocatalysts, namely, AlEt3, AlEt2(OEt), Li-n-Bu, and (PhCH2)MgCl, has been studied for the first time in detail using dynamic EPR spectroscopy. It is shown that after mixing the initial components, paramagnetic structures are formed, which include a fragment containing Co(0) with the electronic configuration 3d9, as well as a fragment bearing an aluminium, lithium, or magnesium atom, depending on the nature of the used cocatalyst. Such bimetallic paramagnetic sites are stabilized by acetylacetonate ligands. In addition, the paramagnetic complex contains the arene molecule(s), and the cobalt atom is bonded with the atom of the corresponding non-transition through the alkyl group of the co-catalyst, in particular through the carbon atom in the α-position with respect to the atom of the non-transition element. Due to the high reactivity of the described intermediates, they, under the conditions of hydrogenation catalysis, are transformed into nano-sized cobalt-containing structures that act as carriers of the catalytically active sites. Furthermore, because of the high reactivity and paramagnetism, such intermediates can be detected only by the EPR technique. The paper describes the whole experimental way of interpreting the EPR signals corresponding to the intermediates, precursors of catalytically active structures. In addition, a possible mathematical model based on the obtained experimental EPR data is presented.
Secondary Amines from Catalytic Amination of Bio-Derived Phenolics over Pd/C and Rh/C: Effect of Operation Parameters
Authors: Ortega Maray , Manrique Raydel , Jiménez Romel , Parreño Miriam , Domine Marcelo E. , Arteaga-Pérez Luis E.
Published: March 2023

The production of renewable chemicals using lignocellulosic biomass has gained significant attention in green chemistry. Among biomass-derived chemicals, secondary amines have emerged as promising intermediates for synthetic applications. Here, we report a systematic study on the reductive amination of phenolics with cyclohexylamine using Pd/C and Rh/C as catalysts. The catalytic tests were performed in batch reactors under different reaction conditions (various: amine concentration (0.1–0.4 mol/L), hydrogen pressure (0–2.5 bar), temperature (80–160 °C), and substituted phenols (phenol, o-cresol, p-cresol, and methoxyphenol)) and using tert-amyl alcohol as a solvent. The experimental observations were consistent with a multi-step mechanism, where hydrogenation of phenol to cyclohexanone is followed by condensation of the ketone with cyclohexylamine to form an imine, which is finally hydrogenated to produce secondary amines. In addition, there was evidence of parallel self-condensation of the cyclohexylamine. The study also supported a limited dehydrogenation capacity of Rh/C, unlike Pd/C, which increases this capacity at higher temperatures generating a higher yield of cyclohexylaniline (up to 15%). The study of the alkylated phenols demonstrated that the nature and propensity of hydrogenation of the phenolic controls their amination. Kinetic analysis revealed reaction orders between 0.4 and 0.7 for H2, indicating its dissociative adsorption. Meanwhile, phenol’s order (between 1–1.8) suggests a single participation of this compound in the hydrogenation step. The order of 0.4 for cyclohexylamine suggests its participation as a surface-abundant species. The apparent activation energies derived from a power law approximation were of 37 kJ/mol and 10 kJ/mol on Pd/C and Rh/C, respectively.
Plant-Biomass-Derived Carbon Materials as Catalyst Support, A Brief Review
Authors: Stepacheva Antonina A. , Markova Mariia E. , Lugovoy Yury V. , Kosivtsov Yury Yu. , Matveeva Valentina G. , Sulman Mikhail G.
Published: March 2023

Carbon materials are widely used in catalysis as effective catalyst supports. Carbon supports can be produced from coal, organic precursors, biomass, and polymer wastes. Biomass is one of the promising sources used to produce carbon-based materials with a high surface area and a hierarchical structure. In this review, we briefly discuss the methods of biomass-derived carbon supported catalyst preparation and their application in biodiesel production, organic synthesis reactions, and electrocatalysis.
The Regulation of Neutrophil Migration in Patients with Sepsis: The Complexity of the Molecular Mechanisms and Their Modulation in Sepsis and the Heterogeneity of Sepsis Patients
Authors: Bruserud Øystein , Mosevoll Knut Anders , Bruserud Øyvind , Reikvam Håkon , Wendelbo Øystein
Published: March 2023

Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Common causes include gram-negative and gram-positive bacteria as well as fungi. Neutrophils are among the first cells to arrive at an infection site where they function as important effector cells of the innate immune system and as regulators of the host immune response. The regulation of neutrophil migration is therefore important both for the infection-directed host response and for the development of organ dysfunctions in sepsis. Downregulation of CXCR4/CXCL12 stimulates neutrophil migration from the bone marrow. This is followed by transmigration/extravasation across the endothelial cell barrier at the infection site; this process is directed by adhesion molecules and various chemotactic gradients created by chemotactic cytokines, lipid mediators, bacterial peptides, and peptides from damaged cells. These mechanisms of neutrophil migration are modulated by sepsis, leading to reduced neutrophil migration and even reversed migration that contributes to distant organ failure. The sepsis-induced modulation seems to differ between neutrophil subsets. Furthermore, sepsis patients should be regarded as heterogeneous because neutrophil migration will possibly be further modulated by the infecting microorganisms, antimicrobial treatment, patient age/frailty/sex, other diseases (e.g., hematological malignancies and stem cell transplantation), and the metabolic status. The present review describes molecular mechanisms involved in the regulation of neutrophil migration; how these mechanisms are altered during sepsis; and how bacteria/fungi, antimicrobial treatment, and aging/frailty/comorbidity influence the regulation of neutrophil migration.