bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Study on the Expression of Testin, p16 and Ki-67 in the Cervical Intraepithelial Neoplasia
Authors: Popiel Aneta , Piotrowska Aleksandra , Sputa-Grzegrzolka Patrycja , Smolarz Beata , Romanowicz Hanna , Dziegiel Piotr , Podhorska-Okolow Marzenna , Kobierzycki Christopher
Published: August 2021

Cervical cancer is one of the most common malignant cancers in women worldwide. The 5-year survival rate is 65%; nevertheless, it depends on race, age, and clinical stage. In the oncogenesis of cervical cancer, persistent HPV infection plays a pivotal role. It disrupts the expression of key proteins as Ki-67, p16, involved in regulating the cell cycle. This study aimed to identify the potential role of testin in the diagnosis of cervical precancerous lesions (CIN). The study was performed on selected archival paraffin-embedded specimens of CIN1 (31), CIN2 (75), and CIN3 (123). Moderate positive correlation was observed between testin and Ki-67 as well as testin and p16 expression in all dysplastic lesions (r = 0.4209, r = 0.5681; p < 0.0001 for both). Statistical analysis showed stronger expression of the testin in dysplastic lesions vs. control group (p < 0.0001); moreover, expression was significantly higher in HSIL than LSIL group (p < 0.0024). In addition, a significantly stronger expression of testin was observed in CIN3 vs. CIN1 and CIN3 vs. CIN2. In our study, expression of Ki-67, p16, and testin increased gradually as the lesion progressed from LSIL to HSIL. The three markers complemented each other effectively, which may improve test sensitivity and specificity when used jointly.
Still a Long Way to Go. Editorial for the Special Issue “Understanding Autism Spectrum Disorder”
Authors: Aguglia Eugenio , Fusar-Poli Laura
Published: August 2021

Although many years have passed since the first descriptions of autism spectrum disorder (ASD) [...]
Chronic Fentanyl Self-Administration Generates a Shift toward Negative Affect in Rats during Drug Use
Authors: Dao Angela , Beacher Nicholas , Mayr Vivian , Montemarano Annalisa , Hammer Sam , West Mark
Published: August 2021

Drug addiction is thought to be driven by negative reinforcement, and it is thought that a shift from positive affect upon initial exposure to negative affect after chronic exposure to a drug is responsible for maintaining self-administration (SA) in addicted individuals. This can be modeled in rats by analyzing ultrasonic vocalizations (USVs), a type of intraspecies communication indicative of affective state based on the frequency of the emission: calls in the 22 kHz range indicate negative affect, whereas calls in the 50 kHz range indicate positive affect. We employed a voluntary chronic, long-access model of fentanyl SA to analyze affective changes in the response to chronic fentanyl exposure. Male Sprague-Dawley rats self-administered either fentanyl (N = 7) or saline (N = 6) for 30 consecutive days and USVs were recorded at four different time points: the day before the first SA session (PRE), the first day of SA (T01), the last day of SA (T30), and the first day of abstinence (ABS). At T01, the ratio of 50 to 22 kHz calls was similar between the fentanyl and saline groups, but at T30, the ratio differed between groups, with the fentanyl group showing significantly fewer 50 kHz calls and more 22 kHz calls relative to saline animals. These results indicate a shift toward a negative affect during drug use after chronic exposure to fentanyl and support negative reinforcement as a main driving factor of opioid addiction.
Weaker Connectivity of the Cortical Networks Is Linked with the Uncharacteristic Gait in Youth with Cerebral Palsy
Authors: Doucet Gaelle E. , Baker Sarah , Wilson Tony W. , Kurz Max J.
Published: August 2021

Cerebral palsy (CP) is the most prevalent pediatric neurologic impairment and is associated with major mobility deficiencies. This has led to extensive investigations of the sensorimotor network, with far less research focusing on other major networks. The aim of this study was to investigate the functional connectivity (FC) of the main sensory networks (i.e., visual and auditory) and the sensorimotor network, and to link FC to the gait biomechanics of youth with CP. Using resting-state functional magnetic resonance imaging, we first identified the sensorimotor, visual and auditory networks in youth with CP and neurotypical controls. Our analysis revealed reduced FC among the networks in the youth with CP relative to the controls. Notably, the visual network showed lower FC with both the sensorimotor and auditory networks. Furthermore, higher FC between the visual and sensorimotor cortices was associated with larger step length (r = 0.74, pFDR = 0.04) in youth with CP. These results confirm that CP is associated with functional brain abnormalities beyond the sensorimotor network, suggesting abnormal functional integration of the brain’s motor and primary sensory systems. The significant association between abnormal visuo-motor FC and gait could indicate a link with visuomotor disorders in this patient population.
Epigenetic Alterations Upstream and Downstream of p53 Signaling in Colorectal Carcinoma
Authors: Tomicic Maja T. , Dawood Mona , Efferth Thomas
Published: August 2021

Colorectal cancer (CRC) belongs to the most common tumor types, and half of all CRC harbor missense mutations in the TP53 tumor suppressor gene. In addition to genetically caused loss of function of p53, epigenetic alterations (DNA methylation, histone modifications, micro-RNAs) contribute to CRC development. In this review, we focused on epigenetic alterations related to the entire p53 signaling pathway upstream and downstream of p53. Methylation of genes which activate p53 function has been reported, and methylation of APC and MGMT was associated with increased mutation rates of TP53. The micro-RNA 34a activates TP53 and was methylated in CRC. Proteins that regulate TP53 DNA methylation, mutations, and acetylation of TP53-related histones were methylated in CRC. P53 regulates the activity of numerous downstream proteins. Even if TP53 is not mutated, the function of wildtype p53 may be compromised if corresponding downstream genes are epigenetically inactivated. Thus, the role of p53 for CRC development, therapy response, and survival prognosis of patients may be much more eminent than previously estimated. Therefore, we propose that novel diagnostic devices measuring the entirety of genetic and epigenetic changes in the “p53 signalome” have the potential to improve the predictive and prognostic power in CRC diagnostics and management.
Histone Modifying Enzymes as Targets for Therapeutic Intervention in Oesophageal Adenocarcinoma
Authors: Pickering Oliver J. , Breininger Stella P. , Underwood Timothy J. , Walters Zoë S.
Published: August 2021

Oesophageal adenocarcinoma (OAC) has a dismal prognosis, where curable disease occurs in less than 40% of patients, and many of those with incurable disease survive for less than a year from diagnosis. Despite the widespread use of systematic chemotherapy in OAC treatment, many patients receive no benefit. New treatments are urgently needed for OAC patients. There is an emerging interest in epigenetic regulators in cancer pathogenesis, which are now translating into novel cancer therapeutic strategies. Histone-modifying enzymes (HMEs) are key epigenetic regulators responsible for dynamic covalent histone modifications that play roles in both normal and dysregulated cellular processes including tumorigenesis. Several HME inhibitors are in clinical use for haematological malignancies and sarcomas, with numerous on-going clinical trials for their use in solid tumours. This review discusses the current literature surrounding HMEs in OAC pathogenesis and their potential use in targeted therapies for this disease.
Photoelectrochemical Oxidation in Ambient Conditions Using Earth-Abundant Hematite Anode: A Green Route for the Synthesis of Biobased Polymer Building Blocks
Authors: Kawde Anurag , Sayed Mahmoud , Shi Qi , Uhlig Jens , Pullerits Tönu , Hatti-Kaul Rajni
Published: August 2021

This study demonstrates the use of a photoelectrochemical device comprising earth-abundant hematite photoanode for the oxidation of 5-hydroxymethylfurfural (5-HMF), a versatile bio-based platform chemical, under ambient conditions in the presence of an electron mediator. The results obtained in this study showed that the hematite photoanode, upon doping with fluorine, can oxidize water even at lower pH (4.5 and 9.0). For 5-HMF oxidation, three different pH conditions were investigated, and complete oxidation to 2,5-furandicarboxylic acid (FDCA) via 5-hydroxymethyl-2-furancarboxylic acid (HMFCA) was achieved at pH above 12. At lower pH, the oxidation followed another route via 2,5-diformylfuran (DFF), yielding 5-formyl-2-furancarboxylic acid (FFCA) as the main product. Using the oxidized intermediates as substrates showed DFF to be most efficiently oxidized to FDCA. We also show that, at pH 4.5, the addition of the laccase enzyme promoted the oxidation of 5-HMF to FFCA.
Unraveling the IGF System Interactome in Sarcomas Exploits Novel Therapeutic Options
Authors: Mancarella Caterina , Morrione Andrea , Scotlandi Katia
Published: August 2021

Aberrant bioactivity of the insulin-like growth factor (IGF) system results in the development and progression of several pathologic conditions including cancer. Preclinical studies have shown promising anti-cancer therapeutic potentials for anti-IGF targeted therapies. However, a clear but limited clinical benefit was observed only in a minority of patients with sarcomas. The molecular complexity of the IGF system, which comprises multiple regulators and interactions with other cancer-related pathways, poses a major limitation in the use of anti-IGF agents and supports the need of combinatorial therapeutic strategies to better tackle this axis. In this review, we will initially highlight multiple mechanisms underlying IGF dysregulation in cancer and then focus on the impact of the IGF system and its complexity in sarcoma development and progression as well as response to anti-IGF therapies. We will also discuss the role of Ephrin receptors, Hippo pathway, BET proteins and CXCR4 signaling, as mediators of sarcoma malignancy and relevant interactors with the IGF system in tumor cells. A deeper understanding of these molecular interactions might provide the rationale for novel and more effective therapeutic combinations to treat sarcomas.
Multiple Targets for Oxysterols in Their Regulation of the Immune System
Authors: Reinmuth Lisa , Hsiao Cheng-Chih , Hamann Jörg , Rosenkilde Mette , Mackrill John
Published: August 2021

Oxysterols, or cholesterol oxidation products, are naturally occurring lipids which regulate the physiology of cells, including those of the immune system. In contrast to effects that are mediated through nuclear receptors or by epigenetic mechanism, which take tens of minutes to occur, changes in the activities of cell-surface receptors caused by oxysterols can be extremely rapid, often taking place within subsecond timescales. Such cell-surface receptor effects of oxysterols allow for the regulation of fast cellular processes, such as motility, secretion and endocytosis. These cellular processes play critical roles in both the innate and adaptive immune systems. This review will survey the two broad classes of cell-surface receptors for oxysterols (G-protein coupled receptors (GPCRs) and ion channels), the mechanisms by which cholesterol oxidation products act on them, and their presence and functions in the different cell types of the immune system. Overall, this review will highlight the potential of oxysterols, synthetic derivatives and their receptors for physiological and therapeutic modulation of the immune system.
Parameter Estimation of Partially Observed Turbulent Systems Using Conditional Gaussian Path-Wise Sampler
Authors: Zhang Ziheng , Chen Nan
Published: August 2021

Parameter estimation of complex nonlinear turbulent dynamical systems using only partially observed time series is a challenging topic. The nonlinearity and partial observations often impede using closed analytic formulae to recover the model parameters. In this paper, an exact path-wise sampling method is developed, which is incorporated into a Bayesian Markov chain Monte Carlo (MCMC) algorithm in light of data augmentation to efficiently estimate the parameters in a rich class of nonlinear and non-Gaussian turbulent systems using partial observations. This path-wise sampling method exploits closed analytic formulae to sample the trajectories of the unobserved variables, which avoid the numerical errors in the general sampling approaches and significantly increase the overall parameter estimation efficiency. The unknown parameters and the missing trajectories are estimated in an alternating fashion in an adaptive MCMC iteration algorithm with rapid convergence. It is shown based on the noisy Lorenz 63 model and a stochastically coupled FitzHugh–Nagumo model that the new algorithm is very skillful in estimating the parameters in highly nonlinear turbulent models. The model with the estimated parameters succeeds in recovering the nonlinear and non-Gaussian features of the truth, including capturing the intermittency and extreme events, in both test examples.
Electron Lone-Pairs Stereochemistry and Drastic van der Waals and Pressure Effects in AsF3 from First Principles
Authors: Galy Jean , Matar Samir F.
Published: August 2021

AsF3E as a representative of a molecular crystal has been chosen to find the precise localization of the lone pair (LP) E centroid 4s2 of As3+ and to enlighten the behavior of lone pair triplets of fluorine atoms. Starting from stereochemistry rationale, Density Functional (DFT) electronic structure calculations yielding the electron localization (ELF) mapping led to precise large crystal structure evolutions from basic X-rays data (V = 267.2Å3 at 193K), to (V = 230.5Å3) and under Van der Waals forces (DEW) V = 206.4Å3, and then under pressure P, all illustrated with ELF maps and band structures. Calibrated pressures up to 100 GPa exhibit the remarkable shrinking of all inter-atomic distances including As-E from 0.94Å down to 0.46Å, while the major three bonds As-F1, As-F2 and As-F3 are continuously expanding. The resulting picture of the application of pressure on AsF3 molecular structure leads to the progressive immersion of the 4s2 doublet within the electronic cloud with an original new status of As with its 4s2 E expressed as [AsE]3+. The expanded size becomes close to big LP-free K+, Ba2+ cations or LP characterized bismuth cation: [BiE]3+, which appear inserted in cubes or square anti-prisms with coordination number 8 (CN8) or in tricapped trigonal prisms with CN9.
Parallel Outbreaks of Deadly Pathogens (SARS-CoV-2, H5N8, EVD, Black Fungi) around East Africa and Asia in 2021: Priorities for Outbreak Management with Socio-Economic and Public Health Impact
Authors: Khan Afroza , Ema Nayeema Talukder , Rakhi Nadira Naznin , Saha Otun , Ahamed Tamer , Rahaman Md. Mizanur
Published: August 2021

Concurrent waves of coronavirus disease, Ebola virus disease, avian influenza A, and black fungus are jeopardizing lives in some parts of Africa and Asia. From this point of view, this review aims to summarize both the socio-economic and public health implications of these parallel outbreaks along with their best possible management approaches. Online databases (PubMed/PMC/Medline, Publons, ResearchGate, Scopus, Google Scholar, etc.) were used to collect the necessary information regarding these outbreaks. Based on the reports published and analyses performed so far, the long-lasting impacts caused by these simultaneous outbreaks on global socio-economical and public health status can be conceived from the past experiences of outbreaks, especially the COVID-19 pandemic. Moreover, prolonged restrictions by the local government may lead to food insecurity, global recession, and an enormous impact on the mental health of people of all ages, specifically in developing countries. Such overwhelming effects have already been reported to be declining the national growth of the economy as well as increasing political insecurity and shortage of basic needs. Although various actions have already been taken, including vaccination, clinical management and further research, social distancing, lockdown, etc., to improve the situation, the emerging variants and associated genetic mutations may make containment difficult, worsening the situation again. So, considering the current mutational dynamics of the pathogens and past experiences, perpetual preparedness along with updated clinical management backed by epidemiological studies and innovative scientific effort are inevitable to combat the simultaneous waves of multiple infectious diseases.
Controlled Morphology and Its Effects on the Thermoelectric Properties of SnSe2 Thin Films
Authors: Siyar Muhammad , Farid Maroosha , Khan Haad , Umar Malik Adeel , Tanveer Waqas Hassan , Safdar Amna
Published: August 2021

In the last few years, the thermoelectric properties of tin selenide (SnSe) have been explored in much detail due to its high efficiency and green nature, being free of Te and Pb. In the same chalcogenide family, SnSe2 is also a layered structured material, but its thermoelectric potential has not been widely explored experimentally. Since SnSe2 has the layered structure, its electrical transport properties may strongly be affected by its microstructure and morphology. Here, we report the effect of reaction time on the structure, phase, and morphology of the SnSe2 during solvothermal synthesis process. We have studied four SnSe2 samples with different reaction times. The sample obtained after 16 h of reaction time was named as M1, for 20 h M2, similarly for 24 h was M3 and for 48 hours’ time, the sample was named as M4. We investigated its thermoelectric properties and found that phase purity and morphology can affect the thermoelectric performance of the synthesized samples. The peak power factor (PF) value along the ab plane was (0.69 μWcm−1K−2) for the M4 sample at 575 K, which was the highest among all the measured samples. The comparatively larger PF value of sample M4 can be related to the increase in its electrical conductivity due to increase in phase purity and band gap reduction.
Granular Cell Dermatofibroma: When Morphology Still Matters
Authors: Cazzato Gerardo , Colagrande Anna , Cimmino Antonietta , Marrone Maricla , Stellacci Alessandra , Arezzo Francesca , Lettini Teresa , Resta Leonardo , Ingravallo Giuseppe
Published: August 2021

Dermatofibroma, also known as “fibrous histiocytoma”, is one of the most common cutaneous soft-tissue tumors. Many variants of dermatofibromas have been described, and knowledge of these variations is important to avoid misdiagnosis of a possibly more aggressive tumor. Histological features of different variants can coexist in the same lesion, but typical common fibrous histiocytoma features are generally found, at least focally, in all cases. However, when cellular changes make up the majority of the lesion, the histopathological diagnosis can become more complex and requires immunohistochemical investigations for correct nosographic classification. We present the case of a cutaneous fibrous histiocytoma, “granular cell” variant, found on the left leg of a 74-year-old woman.
Cities on the Coast and Patterns of Movement between Population Growth and Diffusion
Authors: Kovalevsky Dmitry V. , Volchenkov Dimitri , Scheffran Jürgen
Published: August 2021

Sea level rise and high-impact coastal hazards due to on-going and projected climate change dramatically affect many coastal urban areas worldwide, including those with the highest urbanization growth rates. To develop tailored coastal climate services that can inform decision makers on climate adaptation in coastal cities, a better understanding and modeling of multifaceted urban dynamics is important. We develop a coastal urban model family, where the population growth and urbanization rates are modeled in the framework of diffusion over the half-bounded and bounded domains, and apply the maximum entropy principle to the latter case. Population density distributions are derived analytically whenever possible. Steady-state wave solutions balancing the width of inhabited coastal zones, with the skewed distributions maximizing population entropy, might be responsible for the coastward migrations outstripping the demographic development of the hinterland. With appropriate modifications of boundary conditions, the developed family of diffusion models can describe coastal urban dynamics affected by climate change.
Coarse-Grained Pruning of Neural Network Models Based on Blocky Sparse Structure
Authors: Huang Lan , Zeng Jia , Sun Shiqi , Wang Wencong , Wang Yan , Wang Kangping
Published: August 2021

Deep neural networks may achieve excellent performance in many research fields. However, many deep neural network models are over-parameterized. The computation of weight matrices often consumes a lot of time, which requires plenty of computing resources. In order to solve these problems, a novel block-based division method and a special coarse-grained block pruning strategy are proposed in this paper to simplify and compress the fully connected structure, and the pruned weight matrices with a blocky structure are then stored in the format of Block Sparse Row (BSR) to accelerate the calculation of the weight matrices. First, the weight matrices are divided into square sub-blocks based on spatial aggregation. Second, a coarse-grained block pruning procedure is utilized to scale down the model parameters. Finally, the BSR storage format, which is much more friendly to block sparse matrix storage and computation, is employed to store these pruned dense weight blocks to speed up the calculation. In the following experiments on MNIST and Fashion-MNIST datasets, the trend of accuracies with different pruning granularities and different sparsity is explored in order to analyze our method. The experimental results show that our coarse-grained block pruning method can compress the network and can reduce the computational cost without greatly degrading the classification accuracy. The experiment on the CIFAR-10 dataset shows that our block pruning strategy can combine well with the convolutional networks.
The Impact of Higher Education Institutions in Low-Density Territories
Authors: Pereira Cristina , Mourato Joaquim , Alves João , Serafim Miguel
Published: August 2021

This article highlights the relevance of the location of HEIs in low-density territories in Portugal, using the Polytechnic Institute of Portalegre as a case study. Based on the American Council Education model and following a surveying approach to faculty, staff, and students, this research accounts for the total spending of incoming academics, other nonlocal university members and their visitors, that positively impacts regional development. A demand-side approach was followed so that indirect and induced effects could also be estimated. The main aim of this research paper is to quantify the total impact arising from the location of the Polytechnic in a given region, measured by economic and social indicators such as the financial return from public funds invested in the region, the number of jobs created, and the impact on the local gross domestic product. The results show an impact of more than EUR 17 million in the territory where the Polytechnic operates, representing 3.68% of the local GDP. The institution was also found to be the third major employer in the region, responsible for the creation of 471 jobs that account for 2.25% of the local economically active population.
Energy Savings by Optimization of Thrusters Allocation during Complex Ship Manoeuvres
Authors: Artyszuk Jarosław , Zalewski Paweł
Published: August 2021

The International Maritime Organization adopted a strategy to reduce the total annual GHG emissions from international shipping by at least 50% by 2050, compared to 2008 levels. The European Union proposed an even farther reaching transformation: the European Commission adopted a set of proposals to make the EU’s transport policies fit for reducing net greenhouse gas emissions by at least 55% by 2030, compared to 1990 levels. Therefore, all industrial actions in line and consistent with these strategies are essential. One of such activities may be a gradual transition from the most common independent controls of transport ships’ thrusters, propellers, and rudders to an integrated, power optimized, 3 degrees of freedom joystick control. In this paper, the full mission bridge simulator (FMBS) research on potential energy savings and, consequently, a GHG emission reduction, while steering a RoPax twin-screw ferry equipped with bow thrusters by a joystick control, is presented. The task of navigators engaged in the research was to steer the vessel either via classic engine, rudder, and thruster levers or via a joystick while (1) following the predefined straight track, (2) rotating at the turning area, and (3), finally, crabbing (moving sideways) until stopping at the quay fenders. The conclusions are that energy savings of approximately 10% can be expected for berthing manoeuvres controlled by a joystick, compared to independent actuators’ controls. These conclusions have been drawn from a statistical analysis of the ship’s energy consumption during typical manoeuvring phases of 18 berthing operations performed in FMBS.
A Novel Automatic Method for the Urban Canyon Parametrization Needed by Turbulence Numerical Simulations for Wind Energy Potential Assessment
Authors: Salvadori Luca , Di Bernardino Annalisa , Querzoli Giorgio , Ferrari Simone
Published: August 2021

The energy transition to more sustainable forms is currently ongoing worldwide, because of the environmental impacts produced by the non-renewable energy sources employed in the last decades. Among the main alternatives, wind plays a key role and, nowadays, innovative instruments, such as small-scale turbines allow for installation of wind turbines in urban areas. Their energy potential assessment requires high-accuracy simulations of the turbulent flows in the urban canopy layer, which, in turn, require detailed information about the geometrical properties of the basic element to classify urban surfaces, i.e., the urban canyon, often not available. In this work, we propose a novel automatic method, based on Voronoi graph, to univocally identify urban canyons and to extract their geometrical parameters from online available GIS (Geographic Information System) data, and test it on four European cities that differ in size, story and location. Results show the capability of the method to identify the single urban canyon and to properly extract its geometrical parameters, which tend to assume similar values for the largest cities. Moreover, we first attempt to propose and test some curves to generally describe the data probability distribution, which may be useful for turbulence simulations for urban wind energy assessment and planning. The best results are found for the canyon aspect ratio.
Comparison of the Efficiency and Load Power in Periodic Wireless Power Transfer Systems with Circular and Square Planar Coils
Authors: Stankiewicz Jacek Maciej , Choroszucho Agnieszka
Published: August 2021

In the article, a wireless charging system with the use of periodically arranged planar coils is presented. The efficiency of two wireless power transfer (WPT) systems with different types of inductors, i.e., circular and square planar coils is compared, and two models are proposed: analytical and numerical. With the appropriate selection of a load resistance, it is possible to obtain either the maximum efficiency or the maximum power of a receiver. Therefore, the system is analyzed at two optimum modes of operation: with the maximum possible efficiency and with the highest power transmitted to the load. The analysis of many variants of the proposed wireless power transfer solution was performed. The aim was to check the influence of the geometry of the coils and their type (circular or square) on the efficiency of the system. Changes in the number of turns, the distance between the coils (transmit and receive) as well as frequency are also taken into account. The results obtained from analytical and numerical analysis were consistent; thus, the correctness of the adopted circuit and numerical model (with periodic boundary conditions) was confirmed. The proposed circuit model and the presented numerical approach allow for a quick estimate of the electrical parameters of the wireless power transmission system. The proposed system can be used to charge many receivers, e.g., electrical cars on a parking or several electronic devices. Based on the results, it was found that the square coils provide lower load power and efficiency than compared to circular coils in the entire frequency range and regardless of the analyzed geometry variants. The results and discussion of the multivariate analysis allow for a better understanding of the influence of the coil geometry on the charging effectiveness. They can also be valuable knowledge when designing this type of system.
The Physiological Role of Irisin in the Regulation of Muscle Glucose Homeostasis
Authors: Yano Naohiro , Zhao Yu Tina , Zhao Ting C.
Published: August 2021

Irisin is a myokine that primarily targets adipose tissue, where it increases energy expenditure and contributes to the beneficial effects of exercise through the browning of white adipose tissue. As our knowledge has deepened in recent years, muscle has been found to be a major target organ for irisin as well. Several studies have attempted to characterize the role of irisin in muscle to improve glucose metabolism through mechanisms such as reducing insulin resistance. Although they are very intriguing reports, some contradictory results make it difficult to grasp the whole picture of the action of irisin on muscle. In this review, we attempted to organize the current knowledge of the role of irisin in muscle glucose metabolism. We discussed the direct effects of irisin on glucose metabolism in three types of muscle, that is, skeletal muscle, smooth muscle, and the myocardium. We also describe irisin’s effects on mitochondria and its interactions with other hormones. Furthermore, to consider the relationship between the irisin-induced improvement of glucose metabolism in muscle and systemic disorders of glucose metabolism, we reviewed the results from animal interventional studies and human clinical studies.
On Deterministic and Stochastic Multiple Pathogen Epidemic Models
Authors: Vadillo Fernando
Published: August 2021

In this paper, we consider a stochastic epidemic model with two pathogens. In order to analyze the coexistence of two pathogens, we compute numerically the expectation time until extinction (the mean persistence time), which satisfies a stationary partial differential equation with degenerate variable coefficients, related to backward Kolmogorov equation. I use the finite element method in order to solve this equation, and we implement it in FreeFem++. The main conclusion of this paper is that the deterministic and stochastic epidemic models differ considerably in predicting coexistence of the two diseases and in the extinction outcome of one of them. Now, the main challenge would be to find an explanation for this result.
Effect of Plant Diversity on the Occurrence of Diplodia Tip Blight in Natural Secondary Japanese Red Pine Forests
Authors: Cheng Yuan , Liang Jun , Xie Xian , Zhang Xingyao
Published: August 2021

Diplodia tip blight caused by Sphaeropsis sapinea (Fr.) Dyko and B. Sutton is a serious threat to the health of natural secondary Japanese red pine (Pinus densiflora Sieb. et Zucc.) forests. To explore the effect of plant diversity on Diplodia tip blight disease occurrence, the correlation between Diplodia tip blight in P. densiflora and plant diversity in various stand types and vertical structure layers were analyzed by Pearson correlation coefficients and generalized linear model while keeping environmental factors relatively consistent. Disease index of Diplodia tip blight in P. densiflora was positively correlated with the plant diversity indices in the tree layers of P. densiflora–conifer mixed forest. In contrast, it was negatively correlated with the plant diversity indices in the tree layers of P. densiflora–hardwood mixed forest and P. densiflora pure forest. In shrub–herb layers of all stands except the P. densiflora pure forest herb layer, the disease index was positively correlated with the plant diversity indices. The prediction models for the disease index of Diplodia tip blight in the P. densiflora–hardwood mixed forest, P. densiflora–conifer mixed forest, and P. densiflora pure forest were also established. The foregoing results suggest that in the Kunyu Mountains of China, the differences in plant diversity among various stand types and vertical structure layers have different effects on the occurrence of Diplodia tip blight in P. densiflora. Plant diversity largely reflects the occurrence of Diplodia tip blight disease. However, if the effect of plant diversity on Diplodia tip blight is fully reflected, the plant species characteristics, especially tree genetic relationships, should be considered. In this way, plant species diversity structure in natural secondary Japanese red pine forests may be modified through forest management to lower the incidence of Diplodia tip blight.
Critical Factors for Optimum Biodegradation of Bast Fiber’s Gums in Bacterial Retting
Authors: Hossain Mohammad Munir , Siddiquee Shafiquzzaman , Kumar Vijay
Published: August 2021

Bast fiber plants require a post-harvest process to yield useable natural cellulosic fibers, denoted as retting or degumming. It encompasses the degradation of the cell wall’s non-cellulosic gummy substances (NCGs), facilitating fibers separations, setting the fiber’s quality, and determining downstream usages. Due to the inconvenience of traditional retting practices, bacterial inoculum and enzyme applications for retting gained attention. Therefore, concurrent changes of agroclimatic and socioeconomic conditions, the conventional water retting confront multiple difficulties, bast industries become vulnerable, and bacterial agents mediated augmented bio-retting processes trying to adapt to sustainability. However, this process’s success demands a delicate balance among substrates and retting-related biotic and abiotic factors. These critical factors were coupled to degrade bast fibers NCGs in bacterial retting while holistically disregarded in basic research. In this study, a set of factors were defined that critically regulates the process and requires to be comprehended to achieve optimum retting without failure. This review presents the bacterial strain characteristics, enzyme potentials, specific bast plant cell wall’s structure, compositions, solvents, and interactions relating to the maximum NCGs removal. Among plants, associated factors pectin is the primary biding material that determines the process’s dynamics, while its degree of esterification has a proficient effect through bacterial enzymatic degradation. The accomplished bast plant cell wall’s structure, macerating solvents pH, and temperature greatly influence the bacterial retting process. This article also highlights the remediation process of water retting pollution in a biocompatible manner concerning the bast fiber industry’s endurance.
Novel Computations of the Time-Fractional Fisher’s Model via Generalized Fractional Integral Operators by Means of the Elzaki Transform
Authors: Rashid Saima , Hammouch Zakia , Aydi Hassen , Ahmad Abdulaziz Garba , Alsharif Abdullah M.
Published: August 2021

The present investigation dealing with a hybrid technique coupled with a new iterative transform method, namely the iterative Elzaki transform method (IETM), is employed to solve the nonlinear fractional Fisher’s model. Fisher’s equation is a precise mathematical result that arose in population dynamics and genetics, specifically in chemistry. The Caputo and Antagana-Baleanu fractional derivatives in the Caputo sense are used to test the intricacies of this mechanism numerically. In order to examine the approximate findings of fractional-order Fisher’s type equations, the IETM solutions are obtained in series representation. Moreover, the stability of the approach was demonstrated using fixed point theory. Several illustrative cases are described that strongly agree with the precise solutions. Moreover, tables and graphs are included in order to conceptualize the influence of the fractional order and on the previous findings. The projected technique illustrates that only a few terms are sufficient for finding an approximate outcome, which is computationally appealing and accurate to analyze. Additionally, the offered procedure is highly robust, explicit, and viable for nonlinear fractional PDEs, but it could be generalized to other complex physical phenomena.
Megatsunamis Induced by Volcanic Landslides in the Canary Islands: Age of the Tsunami Deposits and Source Landslides
Authors: Ferrer Mercedes , González de Vallejo Luis , Madeira José , Andrade César , García-Davalillo Juan C. , Freitas Maria da Conceição , Meco Joaquín , Betancort Juan F. , Torres Trinidad , Ortiz José Eugenio
Published: August 2021

Evidence for frequent, large landslides on the flanks of the volcanic edifices forming the Canary Islands include outstanding landslide scars and their correlative submarine and subaerial rock and debris avalanche deposits. These landslides involved volumes ranging from tens to hundreds of km3. The sudden entry of large volumes of rock masses in the sea may have triggered tsunamis capable of affecting the source and neighboring islands, with the resulting huge waves dragging coastal and seabed materials and fauna and redepositing them inland. Here, we present new geological evidence and geochronological data of at least five megatsunamis in Tenerife, Lanzarote, and Gran Canaria, triggered by island flank megalandslides, and occasionally explosive eruptions, during the last 1 million years. The exceptional preservation of the megatsunami deposits and the large area they cover, particularly in Tenerife, provide fundamental data on the number of tsunami events and run-ups, and allow proposals on the sources and age of the tsunamis. Tsunami run-up heights up to 290 m above coeval sea level, some of the highest known on Earth in recent geological times, were estimated based on sedimentological, geomorphological, paleontological, and geochronological data. The research results made it possible to estimate the recurrence of tsunamis in the archipelago during the last hundreds of thousands of years, and to establish relationships between tsunami deposits and the probable triggering island flank landslides.
On the Hierarchical Use of Colourants in a 15th Century Book of Hours
Authors: Agostino Angelo , Pellizzi Eleonora , Aceto Maurizio , Castronovo Simonetta , Saroni Giovanna , Gulmini Monica
Published: August 2021

An illuminated Book of Hours (in use in Chalon-sur-Saône) currently owned by the Museo Civico di Arte Antica and displayed in the prestigious Palazzo Madama in Torino (Italy) was investigated by means of optical microscopy, fibre optic reflectance spectroscopy, fibre optic molecular fluorimetry, X-ray fluorescence spectrometry and Raman spectroscopy. The aim of the scientific survey was to expand the knowledge of the manuscript itself and on the materials and techniques employed by Antoine the Lonhy, the versatile itinerant artist who decorated the book in the 15th century. The focus was to reveal the original colourants and to investigate the pigments used in rough retouches which were visible in some of the miniatures. The investigation was carried out in situ by portable instruments according to a non-invasive analytical sequence previously developed. It was evident that the use of different pigments by the master was ruled, at least partially, by a hierarchical scheme in which more precious materials were linked to the most important characters or details in the painted scene.
Growth Characteristics, Phytochemical Contents, and Antioxidant Capacity of Trachyandra ciliata (L.f) Kunth Grown in Hydroponics under Varying Degrees of Salinity
Authors: Ngxabi Sihle , Jimoh Muhali Olaide , Kambizi Learnmore , Laubscher Charles Petrus
Published: August 2021

This study evaluated the effect of salinity and soilless media on the vegetative growth, phytochemicals, and antioxidant capacity of Trachyandra ciliata (wild cabbage) to develop its growth protocol and explore its potential as a natural source of secondary metabolites. Treatments consisted of different concentrations of sodium chloride (NaCl), control- 0 mM, 100 mM, 200 mM, 400 mM, while different in vitro assays were used for phytochemical and antioxidant screenings. Findings from the study showed that low salinity (100 mM) significantly increased chlorophyll content, plant height, leaf number, plant fresh weight, and production of inflorescence, particularly in Peat-Perlite-Vermiculite (PPV) medium. In contrast, the control was the most productive treatment in plant dry weight except for the inflorescence. The highest antioxidant activity was observed in 200 mM of NaCl treatment in combination with PPV medium, which also produced the highest mean values for polyphenols, while 100 mM was the best for flavonols. Therefore, T. ciliata proved to be more productive vegetatively under low salinity in combination with PPV soilless media. A combination of 200 mM + PPV treatment was also recommended for maximum production of antioxidants for T. ciliata.
Effects of Machine-Harvest Interval on Fruit Quality of Fresh Market Northern Highbush Blueberry
Authors: Cai Yixin , Takeda Fumiomi , Foote Brian , DeVetter Lisa Wasko
Published: August 2021

Machine harvesting blueberry (Vaccinium sp.) alleviates labor costs and shortages but can reduce fruit quality. Installation of softer catching surfaces inside modified over-the-row harvesters (modified OTR) and adjusting harvest intervals may improve fruit quality and packout. The objective of this study was to determine the effect of harvest interval on fruit quality of fresh market northern highbush blueberry (Vaccinium corymbosum L.) harvested using a modified OTR. ‘Liberty’ blueberry bushes were harvested by hand or using a modified OTR at 3-, 10-, and 14-day intervals in 2019 and at 7-, 11-, and 14-day intervals in 2020. Hand-harvested ‘Liberty’ had greater packout and firmness than machine-harvested fruit. Machine harvesting at the 3-day interval in 2019, and the 14-day interval in 2020 reduced packout from 70–80% to 60% and 54%, respectively. In 2019, machine harvesting at a 3-day interval overall resulted in fruit with greater firmness, higher titratable acidity (TA), and lower total soluble solids (TSS) and SS/TA, compared to other harvest intervals. In 2020, the 7-day machine-harvest interval had a greater TA and lower TSS/TA, compared to the 11- and 14-day intervals. Overall, modified OTR machine-harvest intervals can be extended to 10–11 days for fresh market northern highbush cultivars such as ‘Liberty’ grown in northwest Washington.
Vaccine Hesitancy towards COVID-19 Vaccination: Investigating the Role of Information Sources through a Mediation Analysis
Authors: Reno Chiara , Maietti Elisa , Di Valerio Zeno , Montalti Marco , Fantini Maria Pia , Gori Davide
Published: August 2021

Mass vaccination campaigns have been implemented worldwide to counteract the SARS-CoV-2/COVID-19 pandemic, however their effectiveness could be challenged by vaccine hesitancy. The tremendous rise in the use of social media have made them acquire a leading role as an information source, thus representing a crucial factor at play that could contribute to increase or mitigate vaccine hesitancy, as information sources play a pivotal role in shaping public opinion and perceptions. The aims of the study were to investigate if information sources could affect the attitude towards COVID-19 vaccination and if they could act as a mediator in the relationship between individual characteristics and vaccine hesitancy. A cross-sectional online survey was conducted by a professional panellist on a representative sample of 1011 citizens from the Emilia-Romagna region in Italy in January 2021. A mediation analysis using structural equation modelling was performed. Our results show how social media directly or indirectly increases vaccine hesitancy towards COVID-19 vaccination, while the opposite effect was observed for institutional websites. Given the global widespread use of social media, their use should be enhanced to disseminate scientifically sound information to a greater audience to counteract vaccine hesitancy, while at the same time continuing to promote and update institutional websites that have proven to be effective in reducing vaccine hesitancy.
Analysis of the Actual One-Month Usage of Portable Listening Devices in College Students
Authors: Kim Gibbeum , Shin Jihun , Song Changgeun , Han Woojae
Published: August 2021

Although contemporary researchers are concerned about overexposure of portable listening devices (PLD) for adolescents and young adults who often prefer listening to music at high levels for a long time, many of these studies have focused on either comparing sound pressure levels of various kinds of earphones or evaluating the recognition of noise-included hearing loss and listening habits through surveys. Further still, current criteria were developed for occupational noise-induced hearing loss, so there are only a few published guidelines for hearing insults due to recreational noise exposure. The present study, therefore, measures actual listening levels and PLD time in college students using a real-time measurement system and applying that gathered scientific data to the internationally recommended noise exposure standards. Thirty-four college students were asked to listen to music similar to their daily lifestyles for 4-weeks. After installing the application, the Google account that linked to the user’s mobile phone was logged into the server communication. When a subject listened to music, the average and maximum listening levels and listening time could then be recognized as his or her Google account ID and stored in the database for analysis. User data was measured at 1-s intervals and delivered to the main server system every 5 s. The data were analyzed as LZeq for mean levels and LCpeak for maximum levels, and also for PLD use time. The mean of the preferred listening level was 68–70 dB SPL for 4 weeks with long enough break times. That is, the listening levels of college students were not high enough to induce instant hearing loss when they used PLD. However, there was a large individual difference in the listening levels and use times. When applied to three recommended noise exposure criteria, the number of exceeded subjects also differed from 0 to 56.72% depending on the criterion. We thus suggest that appropriate and standardized criteria for music-induced hearing loss might be proposed for recreational PLD users.