bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-wide identification, comprehensive characterization of transcription factors, cis-regulatory elements, protein homology, and protein interaction network of DREB gene family in Solanum lycopersicum
Authors: Maqsood Hajra , Munir Faiza , Amir Rabia , Gul Alvina
Published: November 2022

Tomato is a drought-sensitive crop which has high susceptibility to adverse climatic changes. Dehydration-responsive element-binding (DREB) are significant plant transcription factors that have a vital role in regulating plant abiotic stress tolerance by networking with DRE/CRT cis-regulatory elements in response to stresses. In this study, bioinformatics analysis was performed to conduct the genome-wide identification and characterization of DREB genes and promoter elements in Solanum lycopersicum. In genome-wide coverage, 58 SlDREB genes were discovered on 12 chromosomes that justified the criteria of the presence of AP2 domain as conserved motifs. Intron–exon organization and motif analysis showed consistency with phylogenetic analysis and confirmed the absence of the A3 class, thus dividing the SlDREB genes into five categories. Gene expansion was observed through tandem duplication and segmental duplication gene events in SlDREB genes. Ka/Ks values were calculated in ortholog pairs that indicated divergence time and occurrence of purification selection during the evolutionary period. Synteny analysis demonstrated that 32 out of 58 and 47 out of 58 SlDREB genes were orthologs to Arabidopsis and Solanum tuberosum, respectively. Subcellular localization predicted that SlDREB genes were present in the nucleus and performed primary functions in DNA binding to regulate the transcriptional processes according to gene ontology. Cis-acting regulatory element analysis revealed the presence of 103 motifs in 2.5-kbp upstream promoter sequences of 58 SlDREB genes. Five representative SlDREB proteins were selected from the resultant DREB subgroups for 3D protein modeling through the Phyre2 server. All models confirmed about 90% residues in the favorable region through Ramachandran plot analysis. Moreover, active catalytic sites and occurrence in disorder regions indicated the structural and functional flexibility of SlDREB proteins. Protein association networks through STRING software suggested the potential interactors that belong to different gene families and are involved in regulating similar functional and biological processes. Transcriptome data analysis has revealed that the SlDREB gene family is engaged in defense response against drought and heat stress conditions in tomato. Overall, this comprehensive research reveals the identification and characterization of SlDREB genes that provide potential knowledge for improving abiotic stress tolerance in tomato.
Sub-surface drip fertigation improves seed cotton yield and monetary returns
Authors: Singh Kulvir , Singh Prabhsimran , Singh Manpreet , Mishra Sudhir Kumar , Iqbal Rashid , Al-Ashkar Ibrahim , Habib-ur-Rahman Muhammad , El Sabagh Ayman
Published: November 2022

Surface flood (SF) method is used to irrigate cotton in India, which results in huge wastage of water besides leaching of nutrients. This necessitates the adoption of efficient management strategies to save scarce water without compromising the yield. Therefore, a 2-year field investigation was conducted under two climatic regimes (Faridkot and Abohar) to study the effect of sub-surface drip fertigation (SSDF) on seed cotton yield (SCY), water productivity, nitrogen use efficiency (NUE), and economic parameters in comparison with SF and surface drip fertigation (SDF). The field experiment had a total of eight treatments arranged in a randomized complete block design. Three levels of sub-surface drip irrigation [(SSDI); i.e., 60%, 80%, and 100% of crop evapotranspiration (ETc)] and two N fertigation levels [100% recommended dose of nitrogen (RDN; i.e., 112.5 kg N ha-1) and 75% RDN] made up six treatments, while SF (Control 1) and SDF at 80% ETc (Control 2), both with 100% of RDN, served as the controls. Among irrigation regimes, the SSDI levels of 80% ETc and 100% ETc recorded 18.7% (3,240 kg ha-1) and 21.1% (3,305 kg ha-1) higher SCY compared with SF (2,728 kg ha-1). Water use efficiency under SF (57.0%) was reduced by 34.2%, 40.8%, and 38.2% compared with SSDI’s 60 (76.5%), 80 (80.3%), and 100% ETc (78.8%), respectively. Among fertigation levels, NUE was higher by 19.2% under 75% (34.1 kg SCY kg-1 N) over 100% RDN (28.6 kg SCY kg-1 N), but later it also registered 11.9% higher SCY, indicating such to be optimum for better productivity. SSDF at 80% ETc along with 112.5 kg N ha-1 recorded 26.6% better SCY (3455 kg ha-1) and 18.5% higher NUE (30.7 kg SCY kg-1 N) over SF. These findings demonstrate that the application of SSDF could save irrigation water, enhance SCY, and improve the farmers’ returns compared with SF. Therefore, in northwestern India, SSDF at 80% ETc along with 112.5 kg N ha-1 could be a novel water-savvy concept which would be immensely helpful in enhancing cotton productivity.
Chromatin dynamics associated with seed desiccation tolerance/sensitivity at early germination in Medicago truncatula
Authors: Sano Naoto , Malabarba Jaiana , Chen Zhijuan , Gaillard Sylvain , Windels David , Verdier Jerome
Published: November 2022

Desiccation tolerance (DT) has contributed greatly to the adaptation of land plants to severe water-deficient conditions. DT is mostly observed in reproductive parts in flowering plants such as seeds. The seed DT is lost at early post germination stage but is temporally re-inducible in 1 mm radicles during the so-called DT window following a PEG treatment before being permanently silenced in 5 mm radicles of germinating seeds. The molecular mechanisms that activate/reactivate/silence DT in developing and germinating seeds have not yet been elucidated. Here, we analyzed chromatin dynamics related to re-inducibility of DT before and after the DT window at early germination in Medicago truncatula radicles to determine if DT-associated genes were transcriptionally regulated at the chromatin levels. Comparative transcriptome analysis of these radicles identified 948 genes as DT re-induction-related genes, positively correlated with DT re-induction. ATAC-Seq analyses revealed that the chromatin state of genomic regions containing these genes was clearly modulated by PEG treatment and affected by growth stages with opened chromatin in 1 mm radicles with PEG (R1P); intermediate openness in 1 mm radicles without PEG (R1); and condensed chromatin in 5 mm radicles without PEG (R5). In contrast, we also showed that the 103 genes negatively correlated with the re-induction of DT did not show any transcriptional regulation at the chromatin level. Additionally, ChIP-Seq analyses for repressive marks H2AK119ub and H3K27me3 detected a prominent signal of H3K27me3 on the DT re-induction-related gene sequences at R5 but not in R1 and R1P. Moreover, no clear H2AK119ub marks was observed on the DT re-induction-related gene sequences at both developmental radicle stages, suggesting that silencing of DT process after germination will be mainly due to H3K27me3 marks by the action of the PRC2 complex, without involvement of PRC1 complex. The dynamic of chromatin changes associated with H3K27me3 were also confirmed on seed-specific genes encoding potential DT-related proteins such as LEAs, oleosins and transcriptional factors. However, several transcriptional factors did not show a clear link between their decrease of chromatin openness and H3K27me3 levels, suggesting that their accessibility may also be regulated by additional factors, such as other histone modifications. Finally, in order to make these comprehensive genome-wide analyses of transcript and chromatin dynamics useful to the scientific community working on early germination and DT, we generated a dedicated genome browser containing all these data and publicly available at https://iris.angers.inrae.fr/mtseedepiatlas/jbrowse/?data=Mtruncatula.
The stimulation mechanism of students’ entrepreneurial intention in entrepreneurship course: A trait activation theory perspective
Authors: Wu Jun , Pan Wenhao , Chen Shuqiu , Deng Baijun
Published: November 2022

This study examines the extent to which flow experience inhibits/enhances the effects of students’ creativity on their entrepreneurial intentions. This study provides evidence to support the contention that flow experience moderates the relationship between creativity and entrepreneurial intention by reference to a field survey of 226 Chinese college students in six college classes. Adopted by a hierarchical regression, this study found that creativity has a significant positive impact on entrepreneurial behavior. Within the subdimensions of flow, Intrinsic work motivation and Work enjoyment plays a significant positive moderating role in the relationship between creativity and entrepreneurial behavior, while absorption does not have such moderating effect. These findings reveal the process and mechanism by which creativity affects entrepreneurial intention and the associated psychological contingency factors.
Moving away from deficiency models: Gradiency in bilingual speech categorization
Authors: Kutlu Ethan , Chiu Samantha , McMurray Bob
Published: November 2022

For much of its history, categorical perception was treated as a foundational theory of speech perception, which suggested that quasi-discrete categorization was a goal of speech perception. This had a profound impact on bilingualism research which adopted similar tasks to use as measures of nativeness or native-like processing, implicitly assuming that any deviation from discreteness was a deficit. This is particularly problematic for listeners like heritage speakers whose language proficiency, both in their heritage language and their majority language, is questioned. However, we now know that in the monolingual listener, speech perception is gradient and listeners use this gradiency to adjust subphonetic details, recover from ambiguity, and aid learning and adaptation. This calls for new theoretical and methodological approaches to bilingualism. We present the Visual Analogue Scaling task which avoids the discrete and binary assumptions of categorical perception and can capture gradiency more precisely than other measures. Our goal is to provide bilingualism researchers new conceptual and empirical tools that can help examine speech categorization in different bilingual communities without the necessity of forcing their speech categorization into discrete units and without assuming a deficit model.
Cumulative exposure to traumatic events and craving among women in residential treatment for substance use disorder: The role of emotion dysregulation and mindfulness disposition
Authors: Sanchez Mariana , Amaro Hortensia
Published: November 2022

Objective

Strong evidence links exposure to traumatic life events with subsequent substance use disorders (SUD). Compared to men, women in SUD treatment are more likely to have a history of trauma, characterized in part by emotion dysregulation known to negatively influence treatment outcomes. Existing research has been conducted with predominantly male and non-Hispanic White samples, with an emphasis on adverse childhood experiences. Little is known about how exposure to cumulative traumatic events across the lifespan affects emotion dysregulation and how this may influence craving, particularly among racial and ethnic minoritized women with SUD. Mindfulness disposition may serve as a protective factor that could buffer the impact of trauma exposure and emotion dysregulation on substance use craving among underrepresented minoritized women with SUD. This study examined the association between cumulative exposure to traumatic events, emotion dysregulation, and mindfulness disposition on substance use craving in a sample of predominantly Hispanic and non-Hispanic Black women in residential treatment for SUD.

Method

Cross-sectional data were analyzed for a baseline sample of 241 women (56.96% Hispanic, 20.7% non-Hispanic White, 20.7% non-Hispanic Black; age: M = 32.11) entering a SUD residential treatment facility who agreed to participate in a parent randomized controlled trial.

Results

Findings indicated that greater cumulative exposure to traumatic events and emotion dysregulation were associated with higher levels of craving. Cumulative traumatic event exposure was indirectly associated with higher craving via lower levels in the mindfulness dimension of acting with awareness. Interaction effects also revealed greater exposure to traumatic events was associated with higher levels of craving among women with low and average (but not high) levels of mindfulness disposition.

Conclusion

These findings provide insight into the potential benefits of targeting emotion regulation and mindfulness-building strategies such as acting with awareness in interventions among racial-ethnically diverse women with SUD. These strategies may be particularly beneficial among those that have experienced extensive histories of trauma exposure. Overall, knowledge gained from the present study can be particularly valuable towards informing treatment models in minoritized groups that currently experience disparities in treatment utilization and outcomes.
The integrated social cognitive theory with the TAM model: The impact of M-learning in King Saud University art education
Authors: Almogren Abeer S. , Aljammaz Norah A.
Published: November 2022

Technology in higher education now includes a substantial amount of mobile learning (M-learning). M-learning also enables students to use the internet and technology for research, teamwork, and idea sharing. Additionally, in order to use M-learning systems, both students and teachers must accept M-learning. However, not enough research has been done in Saudi Arabia to determine how satisfied students are with their real use of mobile learning for educational purposes. As a result, the current study intends to investigate students’ behavioral intentions to utilize mobile learning, their happiness with the technology, as well as their impressions of how they actually use mobile learning systems. Therefore, this study aimed to develop a new model by integrating social cognition theory and the technology acceptance model to better understand the elements that influence the adoption of mobile learning in higher education (TAM). The majority of the information was gathered through a survey, with 412 university students’ randomly assigned questionnaires. The data analysis tools utilized were SPSS and Smart-PLS3.3.3. The studies proposed research model could, according to the study’s findings, account for 52.5% of the variation in how mobile learning systems were actually used. This information is crucial for understanding how social and educational technology factors affect the actual use of mobile learning systems. With only two hypotheses being rejected, this study created a new model that supported 16 of them. The findings indicated a beneficial relationship between 10 social and educational technology elements. The findings also indicated a favorable impact on students’ behavioral intentions to use and student happiness, which favorably impacts the actual use of M-learning in higher education. In order to improve students’ academic performance via mobile learning, social cognitive theory and the TAM model are combined as a consequence of the study’s empirical results. Therefore, we encourage students to collaborate with their colleagues at higher education institutions and use M-learning in their classrooms.
Impact of social support on college students’ anxiety due to COVID-19 isolation: Mediating roles of perceived risk and resilience in the postpandemic period
Authors: He Tai Bo , Tu Chia Ching , Bai Xue
Published: November 2022

Introduction

Because of the outbreak of COVID-19, several colleges and universities in Xi’an, China, implemented quarantine measures and closed their gates, which increased anxiety among the students.

Methods

The Perceived Social Support Scale, Perceived Risk of COVID-19 Pandemic Scale, Connor–Davidson Resilience Scale, and Self-Rating Anxiety Scale were used for measurements. SPSS26 and AMOS26 (IBM SPSS AMOS Statistics, New York, United States) were used for data analysis. Structural equation modeling (SEM) was used to test the data from the 2,251 respondents and the hypothetical model.

Results

The students’ perceived social support was discovered to have had a significant negative effect on anxiety. The students’ perceived COVID-19 risk and resilience played significant mediating roles in the relationship between social support and anxiety.

Discussion

When college students feel social support and have resilience, they can reduce anxiety during the epidemic stage. Therefore, education administrators and parents should help college students to identify the current situation of the epidemic environment, enrich relevant knowledge.
Association between lipid metabolism and cognitive function in patients with schizophrenia
Authors: Liu Huamin , Huang Zhiwei , Zhang Xiaochun , He Yong , Gu Shanyuan , Mo Dan , Wang Shaoli , Yuan Zelin , Huang Yining , Zhong Qi , Zhou Rui , Wu Keyi , Zou Fei , Wu Xianbo
Published: November 2022

Background

The association between blood lipids and cognitive function in schizophrenia is still controversial. Thus, the present study aimed to verify the association between various lipid parameters and cognitive impairment in schizophrenic patients and potential lipid pathways.

Methods

A total of 447 adult inpatients with schizophrenia were divided into cognitive normal and cognitive impairment groups based on the Mini-Mental State Examination with a cut-off of 26. The blood lipid parameters were defined as abnormal levels based on the guideline. The liquid chromatography-mass spectrometry method was used to preliminarily explore the potential lipid metabolism pathway associated with cognitive impairment.

Results

There were 368 (82.3%) patients who had cognitive impairment. Herein, apolipoprotein B was positively associated with cognitive function in overall patients and age (≥45 and <45 years) and sex subgroups. After excluding patients with hypertension and diabetes, ApoB was still significantly associated with cognitive function in all the patients. The associations between other lipid parameters, including non-high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and triglyceride, and cognitive impairment were heterogeneous in age and sex subgroups. In contrast, total cholesterol and apolipoprotein A1 were not significantly associated with cognitive impairment. Metabolomics analysis showed that metabolic pathway mainly involved sphingolipid metabolism. Meanwhile, sphinganine and 3-dehydrosphinganine were positively correlated with lipid parameters and decreased in patients with cognitive impairment as compared to those with normal cognition.

Conclusions

The present study suggests a positive association between lipids and cognitive function in schizophrenic patients and needs to be further verified by a prospective study.
Genetics, shared environment, or individual experience? A cross-sectional study of the health status following SARS-CoV-2 infection in monozygotic and dizygotic twins
Authors: Rupp Sophia Kristina , Weimer Katja , Goebel-Stengel Miriam , Enck Paul , Zipfel Stephan , Stengel Andreas
Published: November 2022

Background

The clinical presentation of COVID-19 shows a remarkably broad spectrum of symptoms. Although studies with adult twins on SARS-CoV-2 infection are rare so far, the fact that there is a genetic component associated with the highly variable clinical outcomes of COVID-19 has already been highlighted in recent studies investigating potential candidate genes and polymorphisms. This is the first study of adult monozygotic (MZ) and dizygotic (DZ) twins concordantly affected by SARS-CoV-2 infection to estimate variances explained by genetic, shared, and individual environmental components of both somatic and psychological symptoms following SARS-CoV-2 infection.

Materials and methods

Data were collected from 10 adult twin pairs (5 MZ, 5 DZ) in which both twins already had a SARS-CoV-2 infection. A self-designed questionnaire, the Barthel Index, and the Multidimensional Fatigue Inventory (MFI) were used to assess various symptoms and health status following SARS-CoV-2 infection. Intra-class correlations were calculated, and the Falconer formula was used to quantify and differentiate the percentages of genetic influences as well as common environment and personal experiences on the examined traits. In addition, potential factors influencing symptom burden were examined and discussed.

Results

We found high estimated heritability for mental impairment after SARS-CoV-2 infection (h2 = 1.158) and for general fatigue (h2 = 1.258). For symptom burden, reduced activity, and reduced motivation the individual environment appears to have the strongest influence. Other fatigue symptoms are influenced by genetic effects which range between 42.8 and 69.4%.

Conclusion

Both genetics and individual environment play a role in health status after SARS-CoV-2 infection–mental status could be influenced primarily by genetic make-up, whereas for symptom burden and certain fatigue dimensions, non-shared environment could play a more critical role. Possible individual factors influencing the course of the disease were identified. However, gene-environment interactions may still be a source of differences between twins, and the search for candidate genes remains crucial on the road to personalized medicine.
The effects of exercise with nicotine replacement therapy for smoking cessation in adults: A systematic review
Authors: Chen Hui , Yang Yang , Miyai Hanna , Yi Chenju , Oliver Brian G.
Published: November 2022

Objective

This systematic review aimed to evaluate the efficacy of exercise programmes with nicotine replacement therapy (NRT) for smoking cessation in adults.

Introduction

Nicotine addiction is mediated by dopamine. Exercise can also activate the dopamine reward system. Therefore, exercise may effectively facilitate NRT to reduce cigarette cravings and withdrawal symptoms.

Inclusion criteria

Clinical trials between 2000 and 2022 used exercise protocols of any intensity for smoking cessation, in current smokers or recent quitters of both genders, aged 18–70, without severe diseases and pregnancy. Mental disorders were not excluded, as exercise can improve mental health status. Therefore, it may be as effective among people with mental health issues as the general population in preventing nicotine cravings and supporting abstinence.

Methods

Four databases (PubMed, Embase, Cochrane, and Medline) were searched for papers in English using the terms “nicotine replacement therapy’, “exercise,” and “smoking cessation.” Titles and abstracts were screened for potentially eligibility before full texts were reviewed. Sample size, gender, study duration, and age was then extracted. The certainty of the evidence was assessed using Joanna Briggs Institute’s (JBI’s) GRADE approach.

Results

Seventeen studies were identified with a total of 3,191 participants. Three studies are not a randomised control study. There was moderate-high quality evidence that exercise can aid NRT in promoting smoking cessation in the short term. Several studies reported temporary reductions in cravings; however, only one trial reported a decrease in cigarette consumption due to exercise intervention and one demonstrated increased smoking abstinence at 1 year of the intervention.

Conclusion

Exercise with NRT aids smoking cessation in the short term, but no evidence suggests its efficacy in the long term when combined. Future trials should include larger sample sizes and strategies to increase exercise adherence.
Analysis of factors influencing substance use craving among Chinese substance users
Authors: Guo Huijuan , Wang Jizhi , Wang Siyuan , Zhou Jiansong , Wang Xiaoping
Published: November 2022

Background

Substance use has been a serious public safety issue. It not only affects the users’ physical and mental health but is also detrimental to social stability. To improve our understanding of this issue, the present study looked to examine the factors influencing substance use craving and develop interventions to reduce craving and relapse among substance users.

Materials and methods

A total of 502 substance users were included in this study. Socio-demographic characteristics and substance use-related characteristics were recorded using self-developed forms. With regard to psychological traits, we used the self-esteem scale, the experience of shame scale, and the revised Cheek and Buss shyness scale to assess the self-esteem, shame, and shyness of substance users, respectively. The degree of substance use craving of substance users was assessed using the visual analog scale (VAS). Data were analyzed using independent samples T-test, Pearson correlation analysis, and multiple linear regression analysis, as appropriate.

Results

The majority of the substance users were unmarried, employed, and with lower education levels. For substance use-related characteristics, the age of first use was 27.52 ± 8.30 years and the duration of substance use was 12.29 ± 7.72 years. The scores of their self-esteem, shame, and shyness were 25.65 ± 3.19, 57.26 ± 7.82, and 37.8 ± 7.13, respectively. All substance users rated the intensity of their substance use cravings using the VAS, which showed that the mean score was 2.83 ± 1.87. Multiple linear regression analysis showed that substance use craving was positively associated with the frequency of substance use (β = 0.186, P < 0.001), times of substance rehabilitation (β = 0.128, P = 0.003), shyness (β = 0.211, P < 0.001), and shame (β = 0.091, P = 0.033), and negatively associated with self-esteem (β = –0.117, P = 0.008).

Conclusion

Factors and psychological traits related to substance use are important to account for substance use craving and relapse. Thus, our findings are helpful for a better understanding of the extent of substance use cravings among users and the selection of appropriate interventions to control the craving and relapse.
Association between the domestic use of solid cooking fuel and increased prevalence of depression and cognitive impairment in a big developing country: A large-scale population-based study
Authors: Jin Yuming , Zhou Xianghong , Deng Linghui , Xiong Xingyu , Li Yifan , Wei Qiang , Dong Birong , Qiu Shi
Published: November 2022

Background

Previous studies have suggested that air pollution affects physiological and psychological health. Using solid fuel at home is a significant source of indoor air pollution. The associations between solid fuel use and depressive symptoms and cognitive health were unclear among older adults from low- and middle-income countries (LMICs).

Methods

To evaluate the association of solid fuel use with depressive symptoms and cognitive health among older adults, we obtained data from the Longitudinal Aging Study in India (LASI) and excluded subjects younger than 60 years and without critical data (solid fuel use, depressive symptoms, and cognitive health). The 10-item Center for Epidemiologic Studies Depression Scale (CES-D-10) was used to assess depressive symptoms, with more than ten indicative of depression. Cognitive health was assessed using measures from the Health and Retirement Study (HRS), and subjects with the lowest 10th percentile were considered to have cognitive impairment. The participants' responses defined solid fuel use. Multivariable logistic regression, linear regression, subgroup analysis, and interaction tests were performed to appraise the relationship between solid fuel use and depression and cognitive impairment.

Results

A total of 29,789 participants over 60 years old were involved in this study. Almost half of the participants (47.5%) reported using solid fuel for home cooking. Compared with clean fuel use, solid fuel use was related to an increased prevalence of depression [odds ratio (OR) 1.09, 95% CI 1.03–1.16] and higher CES-D-10 scores (β 0.23, 95% CI 0.12–0.35) after fully adjusted covariables. Using solid fuel was also related to a higher risk of cognitive impairment (OR 1.21, 95% CI 1.11–1.32) and a lower cognitive score (β −0.63, 95% CI −0.79 to −0.47) compared with those who used clean fuel. In the subgroup analysis, the prevalence of depression increased in females and non-smokers. The association of solid fuel use with depression and cognitive impairment exists in subgroups of BMI, economic status, caste, living area, education, and drinking.

Conclusions

The use of solid fuel at home was associated with an increased prevalence of depression and cognitive impairment among older adults in India.
A cross-sectional assessment of knowledge, awareness of risk factors, and perceptions of thyroid disease (TD) among adults living in Saudi Arabia – A community based study
Authors: Alhazmi Riyadh A. , Alobaid Abdullah M. , Althunayyan Saqer Mohammed , Syed Wajid , Al-Rawi Mahmood Basil A.
Published: November 2022

Background

The incidence of thyroid diseases has tripled in the last three decades, and the prevalence is rising rapidly irrespective of gender and genetics. This study aimed to assess the Knowledge, awareness of risk factors, and perceptions of thyroid disease among the Saudi Community in Saudi Arabia.

Methods

A cross-sectional, online web-based, survey type study was conducted between November 2021 to January 2022 among residents living in Saudi Arabia. Individuals aged ≥ 18 years who expressed a willingness to complete the survey were included. Descriptive and bivariate analyses were carried out to determine the factors associated with knowledge of thyroid using SPSS version 26.0 software (SPSS Inc., Chicago, IL, U.S.).

Results

Among the participants, the majority of them were females than males (77.5 vs. 22.5%). A total of 78.2% (n = 566) of them were aware of the thyroid. Nearly 44% (n = 312) of respondents are aware that a lump in the neck or swelling is a sign of thyroid disease, followed by pain in the neck 24.6% (n = 178), and difficulty in swallowing 23.8% (n = 172). The mean knowledge score of the thyroid was 4.1 (SD = 3.09), while the score of the mean perception was 33.02 (SD = 6.41). The mean knowledge scores were significantly associated with having previous knowledge of thyroid disease (t = 5.08; p = 0.0001). The gender of the participant and the presence of chronic diseases were found to have no impact on the knowledge score of the thyroid disease (t = −1.18; p = 0.235; t = 1.005; p = 0.315). Additionally, the perceptions score was not significantly associated with the demographics of the participants (p = 0.05).

Conclusion

In this study, Saudi adults reported varying levels of knowledge and perceptions of thyroid disease. Having previous knowledge of the thyroid was significantly associated with the knowledge score. It is necessary to educate people about this rising disease.
Musculoskeletal pain is prevalent in Chinese medical and dental students: A cross-sectional study
Authors: Lin Yunzhi , Zhang Xuehui , Li Hongyan , Huang Ying , Zhang Wenming , Zhang Chaofan
Published: November 2022

Objectives

Musculoskeletal pain (MSP) is a major reason for consultation in primary care and is becoming increasingly prevalent among medical students. There is little research on the current situation of MSP among Chinese medical and dental students. Data on the analysis of risk factors related to MSP are also limited. The objectives of this study were to investigate the prevalence and characteristics of MSP among Chinese medical and dental students and to explore the risk factors for MSP and students' intent to seek medical treatment.

Methods

An anonymous, internet-based, cross-sectional, open survey was distributed to medical and dental students at Fujian Medical University, Fuzhou, China. Data on the demographic information and characteristics of MSP were collected and analyzed. In addition to descriptive statistics, logistic regression was used to analyze significant risk factors contributing to MSP.

Results

A total of 1,178 students responded to the survey (response rate = 79.6%), including 722 medical students and 456 dental students. The age ranged from 16 to 24. There were 553 male students and 625 female students. A total of 523 students reported neck pain (NP, 44.4%), 361 students reported low back pain (LBP, 30.6%), and 182 students reported joint pain (JP, 15.4%). Sixty-six students (5.6%) simultaneously suffered from NP, LBP, and JP. The prevalence of NP (49.1 vs. 41.4%, P = 0.01), LBP (34.6 vs. 28.1%, P = 0.02), and JP (20.2 vs. 12.5%, P < 0.001) was significantly higher in dental students than in medical students. The prevalence of MSP was significantly different among the academic years for NP and LBP (P = 0.02 and P < 0.001, respectively). Univariate and multivariate regression analyses demonstrated that female sex, PSS-10 score, and major of stomatology were risk factors for MSP. Medical and dental students' intention to seek treatment for MSP was low and was significantly associated with the severity of pain.

Conclusions

The prevalence of MSP in Chinese medical and dental students is high, especially for NP and LBP, and is significantly higher in dental students than in medical students. The prevalence of NP and LBP were significantly different among academic grades. Female sex, PSS-10 score, and major of stomatology were risk factors for MSP. Students' intent to seek treatment for MSP was very low and was determined by the severity of pain.
Correlation between clinical trial endpoints of marketed cancer drugs and reimbursement decisions in China
Authors: Ling Kexin , Qin Huli , Feng Yiman , Che Hongxi , Ding Jinxi , Li Wei
Published: November 2022

Objective

This study aimed to assess whether different clinical trial endpoints in pivotal trials of cancer drugs were associated with reimbursement decisions in China.

Materials and methods

Cancer drugs marketed before June 30th, 2021 with publicly available technical review reports for application of drug registration on Center for Drug Evaluation (CDE) website were reviewed. The trial design characteristics and relevant clinical outcomes [e.g., overall survival (OS), progression-free survival (PFS) and objective response rate (ORR)] were extracted from the technical review reports, while the reimbursement decisions were reviewed from National Healthcare Security Administration (NHSA) website. The differences in trial characteristics and clinical outcomes between drugs with positive reimbursement decisions and negative ones were compared by hypothesis test (Pearson's chi-squared test, Fisher's exact test, independent samples t-test and Mann-Whitney U test). The correlation between different clinical trial endpoints and reimbursement decisions was analyzed by multivariate logistic regression.

Results

There were 112 cancer drug indications included in this study. Among these indications, 76 received a positive reimbursement decision, and the most common primary endpoints of them were PFS (42.1%) and ORR (30.3%). Taking PFS (OR = 7.333) and ORR (OR = 5.271) as the primary endpoints were more likely to receive a positive reimbursement decision compared with OS (P = 0.003). The proportion of drugs marketed with phase I (75.0%) and phase II (85.7%) clinical trials receiving positive reimbursement decisions are significantly higher than those marketed with phase III clinical trials (61.3%, P = 0.043). The magnitude of clinical benefit only had subtle influences (Prisk benefit − OS = 0.627, Prisk benefit − PFS = 0.087, Psurvival benefit − OS = 0.545, Psurvival benefit − PFS = 0.189) on the drug reimbursement decisions, however, the drug prices and clinical needs also made a difference on that.

Conclusion

This study found that, in Chinese drug price negotiations from 2017 to 2021, policymakers have focused more on meeting clinical needs and filling therapeutical gaps in National Reimbursement Drug List (NRDL), while requirements for the selection of primary endpoints, clinical trial phases, and clinical benefits have been reduced. In the future, emphasis should be put on the use of surrogate endpoints and clinical benefits.
Decidualization of human endometrial stromal cells requires steroid receptor coactivator-3
Authors: Maurya Vineet K. , Szwarc Maria M. , Lonard David M. , Gibbons William E. , Wu San-Pin , O’Malley Bert W. , DeMayo Francesco J. , Lydon John P.
Published: November 2022

Steroid receptor coactivator-3 (SRC-3; also known as NCOA3 or AIB1) is a member of the multifunctional p160/SRC family of coactivators, which also includes SRC-1 and SRC-2. Clinical and cell-based studies as well as investigations on mice have demonstrated pivotal roles for each SRC in numerous physiological and pathophysiological contexts, underscoring their functional pleiotropy. We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. We show here that, like SRC-2, SRC-3 is expressed in the epithelial and stromal cellular compartments of the human endometrium during the proliferative and secretory phase of the menstrual cycle as well as in cultured HESCs. We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. These predictive bioanalytic results were confirmed by the demonstration that SRC-3 is required for the expansion, migratory and invasive activities of the HESC population, cellular properties that are required in vivo in the formation or functioning of the decidua. Collectively, our results support SRC-3 as an important coregulator in HESC decidualization. Since perturbation of normal homeostatic levels of SRC-3 is linked with common gynecological disorders diagnosed in reproductive age women, this endometrial coregulator—along with its new molecular targets described here—may open novel clinical avenues in the diagnosis and/or treatment of a non-receptive endometrium, particularly in patients presenting non-aneuploid early pregnancy loss.
Short-term trade-offs of organic matter management strategies for smallholder farms
Authors: Ekyaligonza Deous Mary , Tibasiima Thaddeo Kahigwa , Dietrich Phillipp , Kagorora John Patrick , Friedel Jürgen Kurt , Eder Michael , Freyer Bernhard
Published: November 2022

Organic matter management (OMM) strategies such as farmyard manure (FYM) application, legume integration, crop residue incorporation, and alley cropping are recognized for improving soil fertility and crop productivity. However, studies on yield and economics of a combination of such strategies on smallholder farms are generally scarce, yet an understanding of such can enhance adoption. This study analyzed the yield and gross margins of crops grown with OMM strategies in comparison to those grown under inorganic fertilizer application on smallholder farms. Field experiments with five treatments over two short rainy (SR) and two long rainy (LR) seasons were conducted from January 2018 to February 2020 on 10 smallholder farms. The treatments (T) included T1 (control): the inorganic fertilizer application strategy that involved maize monocrop with 50 kg/ha Diammonium phosphate (DAP) application and the OMM strategies (T2-T5). T2: cowpea-maize-bean-maize rotation; T3: cowpea-maize-bean-maize rotation + 2.5 tons/ha FYM; T4: Faidherbia albida alleys + cowpea-maize-bean-maize rotation; and T5: Faidherbia albida alleys + cowpea-maize-bean-maize rotation + 2.5 tons/ha FYM. The maize in T3-T5 was intercropped with Mucuna pruriens. The results indicate that the grain and residue yields in LR were not significantly different among all treatments. The total variable costs, which included monetarized labor and annualized capital costs for the establishment of F. albida were significantly higher under T1 than in T2-T5 during LR2018 and not significantly different from what was observed under T3-T5 in LR2019. The accumulated revenues and gross margins for the four seasons were not significantly different between T1 and the OMM strategies. We conclude that the integration of OMM strategies can give gross margins similar to the 50 kg/ha DAP application. Further, based on the price sensitivity analysis, we conclude that the smallholder farmers could adopt T3 and T4 as the gross margins under these treatments are less affected by grain price fluctuations than in T1, T2, and T5. Since the smallholder farmers can access the planting materials, we recommend the adoption of T3 and T4 on smallholder farms.
Age-related brain atrophy in cats without apparent neurological and behavioral signs using voxel-based morphometry
Authors: Hamamoto Yuji , Yu Yoshihiko , Asada Rikako , Mizuno Satoshi , Hasegawa Daisuke
Published: November 2022

Introduction

Brain atrophy is observed with aging and may cause cognitive decline or dysfunction. Aging cats may demonstrate behavioral changes related to cognitive dysfunction. In the clinical veterinary field, although the conventional region of interest method by manual or semiauto tracing on magnetic resonance imaging is used to detect atrophy of regional structures, such as the hippocampus, it is difficult to assess atrophy globally. Voxel-based morphometry (VBM) has been developed to detect global and regional abnormalities in humans. The purpose of the present study investigates whether the feline brain volume decreases with aging using VBM analysis.

Materials

A total of 65 cats, aged 17–200 months, without apparent neurological and behavioral signs were included in the statistical analysis.

Results

We observed that the gray matter in the bilateral parietal lobes was decreased significantly with aging. The regions that showed decreased volume included the right postcruciate, cingulate gyrus, rostral suprasylvian/ectosylvian gyri, and the left postcruciate gyrus. No significant reduction in white matter was observed. Together, our results show that age-related brain atrophy can be detected using VBM analysis.

Discussion

The age-related atrophy of the parietal cortex may not cause neurological and behavioral signs in cats. Therefore, veterinarians should consider age when assessing the relation between morphometric and functional abnormalities of the parietal cortex in cats.
Investigations on the Potentials of Novel Technologies for Aircraft Fuel Burn Reduction through Aerostructural Optimisation
Authors: Mosca Valerio , Elham Ali
Published: November 2022

A physics-based optimisation framework is developed to investigate the potential advantages of novel technologies on the energy efficiency of a midrange passenger aircraft. In particular, the coupled-adjoint aerostructural analysis and optimisation tool FEMWET is modified to study the effects of active flow control at different load cases for conventional and unconventional wing configurations. This multidisciplinary design optimisation (MDO) framework presents the opportunity to optimise the wing considering static aeroelastic effect and, by its gradient-based method, save substantial computational time compared to high-fidelity tools, keeping a satisfying level of accuracy. Two different configurations are analysed: a forward- and backward-swept wing aircraft, developed inside the Cluster of Excellence SE2A (Sustainable and Energy-Efficient Aviation). The forward-swept configuration is sensitive to the aeroelastic stability effect, and the backward configuration is influenced by the aileron constraint. They may lead to a weight increment. Sensitivity studies show the possible role of key parameters on the optimisation results. The highest fuel weight reduction achievable for the two configurations is 5.6% for the forward-swept wing and 9.8% for the backward configuration. Finally, both optimised wings show higher flexibility.
Effects of Chloropicrin, Dimethyl Disulfide and Metham Sodium Applied Simultaneously on Soil-Born Bacteria and Fungi
Authors: Shi Zhaoai , Zhu Jiahong , Wu Jiajia , Cao Aocheng , Fang Wensheng , Yan Dongdong , Wang Qiuxia , Li Yuan
Published: November 2022

The area used to grow high-value crops is currently decreasing because production in the same soil for many years increases soil-borne diseases that reduce crop yield and quality as well as farmer income. Soil-borne disease is effectively controlled by soil fumigation prior to planting. In this study, the five different types of soils that had been used to grow tomatoes, watermelon, cucumber, ginseng and ginger were collected from field plots with high incidence of soil-borne diseases. This experiment adopts the indoor fumigation method to conduct triple fumigation of chloropicrin(PIC), metham sodium(MS) and dimethyl disulfide(DMDS) on different soil collected to examine changes in the soil microbial community, including pathogenic fungi and bacteria and beneficial microorganisms in order to clarify the impact on the overall structure of soil microbial community while controlling complex and multiple pathogens. High-throughput gene sequencing was used to detect bacterial and fungal taxonomic changes in the treated soils. Triple fumigation significantly reduced the abundance of at least five kinds of pathogenic fungi, Fusarium oxysporum, Mortierella, Neocosmospora, Nitrospira Alternaria and significantly increased the abundance of two kinds of beneficial species, Bacillus and Trichoderma. The research result observed increases and decreases in the biodiversity and richness of beneficial and pathogenic bacteria and fungi in response to triple fumigation of soil that had been used to grow tomatoes, watermelon, cucumber, ginseng and ginger. The most significant effect was observed in the experimental field of Panax notoginseng in Wenshan, Yunnan. Triple fumigation showed good potential to decrease pathogenic bacteria and fungi in soils and improve the disease resistance of soils, and that it has a good application prospect in the field of soil disinfestation.