bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of the 135 bp Intron on Stilbene Synthase VaSTS11 Transgene Expression in Cell Cultures of Grapevine and Different Plant Generations of Arabidopsis thaliana
Authors: Kiselev Konstantin V. , Ogneva Zlata V. , Aleynova Olga A. , Suprun Andrey R. , Ananev Alexey A. , Nityagovsky Nikolay N. , Dubrovina Alexandra S.
Published: April 2023

Modern plant biotechnology often faces the problem of obtaining a stable and powerful vector for gene overexpression. It is known that introns carry different regulatory elements whose effects on transgene expression have been poorly studied. To study the effect of an intron on transgene expression, the stilbene synthase 11 (VaSTS11) gene of grapevine Vitis amurensis Rupr. was selected and overexpressed in grapevine callus cell cultures and several plant generations of Arabidopsis thaliana as two forms, intronless VaSTS11c and intron-containing VaSTS11d. The STS genes play an important role in the biosynthesis of stilbenes, valuable plant secondary metabolites. VaSTS11d contained two exons and one intron, while VaSTS11c contained only two exons, which corresponded to the mature transcript. It has been shown that the intron-containing VaSTS11d was better expressed in several generations of transgenic A. thaliana than VaSTS11c and also exhibited a lower level of cytosine methylation. As a result, the content of stilbenes in the VaSTS11d-transgenic plants was much higher than in the VaSTS11c-transgenic plants. Similarly, the best efficiency in increasing the content of stilbenes was also observed in grapevine cell cultures overexpressing the intron-containing VaSTS11d transcript. Thus, the results indicate that an intron sequence with regulatory elements can have a strong positive effect on both transgene expression level and its biological functions in plants and plant cell cultures.
Precise Sensing of Leaf Temperatures for Smart Farm Applications
Authors: Son Ki-Ho , Sim Han-Sol , Lee Jae-Kyoung , Lee Juhwan
Published: April 2023

Leaf temperature represents the mixed effects of solar radiation, temperature, and CO2 enrichment on the potential production of greenhouse vegetables. However, smart farm applications that monitor and account for changes in leaf temperature are limited. This study developed new hardware and software components for leaf temperature sensing integrated into a conventional smart farm system. We demonstrated a new system to monitor leaf temperatures and improve crop yield at two greenhouse tomato and strawberry farms in South Korea. We observed a rapid decline in leaf temperature at both farms when the indoor air temperature decreased. This pattern often corresponds to stagnant CO2 assimilation. The results suggest that leaf temperature sensing is practical for slow aeration and heating that is required to optimize photosynthetic efficiency, especially in the morning and when leaf temperatures become high (over 25 °C). Specifically, smart farm implementation with leaf temperature sensing increased the yield of tomatoes by 28–43%. Furthermore, our study highlights the need to develop leaf temperature models for smart greenhouse farming that interact with nutrient and water supplies.
Apricot (Prunus armeniaca) Performance under Foliar Application of Humic Acid, Brassinosteroids, and Seaweed Extract
Authors: Al-Saif Adel M. , Sas-Paszt Lidia , Awad Rehab M. , Mosa Walid F. A.
Published: April 2023

The excessive use of chemical fertilizers in fruit orchards has led to numerous problems for the environment, produce quality, and food safety. It also negatively affects soil health, beneficial microorganisms, and ground water quality, hence the resurgence of the application of biostimulants as ecofriendly ways to improve the growth, yield, and fruit quality of tree fruits. The current study was performed during 2021 and 2022 to investigate the influence of foliar spraying of 500, 1000, and 2000 mg/L humic acid (HA); 0.5, 1, and 2 mg/L brassinosteroids (Brs); and 1000, 2000, and 3000 mg/L seaweed extract (SWE) compared with a control (untreated trees) in terms of the performance of an apricot (Prunus armeniaca) cv. Canino. The obtained results show that the spraying of HA, Brs, and SWE positively increased the shoot length, leaf area, leaf chlorophyll content, fruit set, fruit yields, and fruit physical and chemical characteristics, as well as leaf macro- or micronutrients contents compared with those untreated trees during both study years. Moreover, the increase in parameter values was parallel to the increase in the used concentrations of HA, Brs, or SWE, where 2000 mg/L HA, 2 mg/L Brs, and 3000 mg/L SWE were superior to 1000 mg/L HA, 1 mg/L Brs, and 2000 mg/L SWE, which were better than 500 mg/L HA, 0.5 mg/L Brs, and 1000 mg/L SWE.
Exposure Assessment of Ambient PM2.5 Levels during a Sequence of Dust Episodes: A Case Study Coupling the WRF-Chem Model with GIS-Based Postprocessing
Authors: Mancinelli Enrico , Avolio Elenio , Morichetti Mauro , Virgili Simone , Passerini Giorgio , Chiappini Alessandra , Grasso Fabio , Rizza Umberto
Published: April 2023

A sequence of dust intrusions occurred from the Sahara Desert to the central Mediterranean in the second half of June 2021. This event was simulated by means of the Weather Research and Forecasting coupled with chemistry (WRF-Chem) regional chemical transport model (CTM). The population exposure to the dust surface PM2.5 was evaluated with the open-source quantum geographical information system (QGIS) by combining the output of the CTM with the resident population map of Italy. WRF-Chem analyses were compared with spaceborne aerosol observations derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) and, for the PM2.5 surface dust concentration, with the Modern-Era Retrospective analysis for Research and Applications, Version 2 (MERRA-2) reanalysis. Considering the full-period (17–24 June) and area-averaged statistics, the WRF-Chem simulations showed a general underestimation for both the aerosol optical depth (AOD) and the PM2.5 surface dust concentration. The comparison of exposure classes calculated for Italy and its macro-regions showed that the dust sequence exposure varies with the location and entity of the resident population amount. The lowest exposure class (up to 5 µg m−3) had the highest percentage (38%) of the population of Italy and most of the population of north Italy, whereas more than a half of the population of central, south and insular Italy had been exposed to dust PM2.5 in the range of 15–25 µg m−3. The coupling of the WRF-Chem model with QGIS is a promising tool for the management of risks posed by extreme pollution and/or severe meteorological events. Specifically, the present methodology can also be applied for operational dust forecasting purposes, to deliver safety alarm messages to areas with the most exposed population.
Athletic Trainers’ Perceptions of and Experience with Social Determinants of Health
Authors: Picha Kelsey J. , Welch Bacon Cailee E. , Bay R. Curt , Lewis Joy H. , Snyder Valier Alison R.
Published: April 2023

The role that social determinants of health (SDHs) play in athletic healthcare is gaining attention, yet little is known about athletic trainers’ (ATs) perceptions of and encounters with the impact of SDHs. The purpose of this study was to evaluate ATs’ perceptions of various SDHs and their experience treating patients whose health and well-being were influenced by SDHs. This was a cross-sectional, web-based survey completed by 1694 ATs (completion rate = 92.6%; 61.1% female; age = 36.6 ± 10.8 years). The survey consisted of several multipart questions focusing on specific SDHs. Descriptive statistics were used to report frequencies and percentages. Results indicated widespread agreement that SDHs matter to patient health and are of concern in athletic healthcare. The SDHs that ATs most commonly reported encountering included lifestyle choices (n = 1306/1406; 93.0%), social support (n = 1185/1427; 83.0%), income (n = 1167/1502; 77.7%), and access to quality and timely healthcare (n = 1093/1420, 77.0%). The SDHs that ATs least commonly reported having experience with was governmental policy (n = 684/1411; 48%). The perceived importance of SDHs among ATs and their commonly reported experiences managing patient cases in which SDHs negatively influence patients’ health and healthcare suggest that efforts to assess these factors are needed so that strategies to address their influence on athletic healthcare can be identified.
Cross-Cultural Adaptation and Psychometric Properties of the Arabic Version of the Fall Risk Questionnaire
Authors: Alharbi Ahmad A. , Al Amer Hamad S. , Albalwi Abdulaziz A. , Muthaffar Majed Y. , Alshehre Yousef M. , Albalawi Hani F. , Alshaikhi Turky E.
Published: April 2023

A simple, valid, and reliable self-assessment fall-risk questionnaire in Arabic is needed to significantly promote awareness and develop fall-prevention programs. This study translated and adapted the Fall Risk Questionnaire (FRQ) into Arabic and determined its validity and reliability among Saudi Arabian older adults in two phases: (1) cross-culturally adapting the FRQ into Arabic and (2) assessing the adapted questionnaire’s psychometric properties in two sessions with 110 Arabic-speaking participants aged ≥65 years. Pearson’s r showed that the Arabic FRQ had a significant moderate negative relationship with the Berg Balance Scale and fair-to-moderate positive correlations with Five Time Sit to Stand and Time Up and Go. The receiver operating characteristic curve indicated a significant area under the curve = 0.81. The cut-off score was 7.5 and associated with 73.7% sensitivity and 73.6% specificity. Internal consistency was estimated as good, with Cronbach’s α = 0.77. Deletion of item 1 slightly increased Cronbach’s α to 0.78. The Arabic FRQ demonstrated excellent test-retest reliability, with an intraclass correlation coefficient = 0.95 (95% CI: 0.92–0.97). It is highly valid and reliable in providing valuable data for evaluating fall risk in adults aged ≥65 years and for consulting a specialist for further investigation if necessary.
Indirect Methods to Determine the Risk of Damage to the Health of Firefighters and Children Due to Exposure to Smoke Emission from Burning Wood/Coal in a Controlled Environment
Authors: Ocampos Marcelo Sampaio , Leite Luana Carolina Santos , de Pádua Melo Elaine Silva , de Cássia Avellaneda Guimarães Rita , Oliveira Rodrigo Juliano , de Cássia Freitas Karine , Hiane Priscila Aiko , Karuppusamy Arunachalam , do Nascimento Valter Aragão
Published: April 2023

People are constantly exposed to particulate matter and chemicals released during fires. However, there are still few studies on gas and particulate emissions related to exposure to burning firewood and charcoal during forest fires, making it difficult to understand the effects on the health of the population. The objective of this study was to quantify the metal(loid)s present in the smoke from wood and charcoal fires through the deposition of metals in beef topside and pork loin, considering the routes of skin exposure, inhalation, and ingestion, contributing to the understanding of metals in the increase of the risks of cancer and mortality associated with firefighting and children. The concentrations of metals [aluminum (Al), chromium (Cr), copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), molybdenum (Mo), vanadium (V), zinc (Zn)] and metalloids arsenic (As) were determined by inductively coupled plasma–mass spectrometry (ICP OES) after microwave digestion. Moreover, we assessed the associated risk regarding the elemental intake of these elements through the smoke, using the hazard quotient (HQ), hazard index (HI), Total Hazard Index (HIt), and carcinogenic risk (CR). All samples had results for HQ and HIt < 1, indicating a non-potential health risk. However, the carcinogenic risks posed by As and Cr via the three exposure pathways (except for inhalation exposure to children and adults, and by Cr via ingestion and inhalation for children and adults) exceeded the standard threshold. In conclusion, continuous exposure of firefighters or children to smoke from fires containing high concentrations of heavy metals such as As and Cr can be harmful to health. The study used animal tissues; thus, new methods must be developed to quantify the concentration of heavy metals deposited in human tissue when humans are exposed to smoke from fires.
Pollen Molecular Identification from a Long-Distance Migratory Insect, Spodoptera exigua, as Evidenced for Its Regional Pollination in Eastern Asia
Authors: Jia Huiru , Wang Tengli , Li Xiaokang , Zhao Shengyuan , Guo Jianglong , Liu Dazhong , Liu Yongqiang , Wu Kongming
Published: April 2023

Understanding plant-insect interactions requires the uncovering of the host plant use of insect herbivores, but such information is scarce for most taxa, including nocturnal moth species, despite their vital role as herbivores and pollinators. In this study, we determined the plant species visited by an important moth species, Spodoptera exigua, by analyzing attached pollen on migratory individuals in Northeast China. Pollen grains were dislodged from 2334 S. exigua long-distance migrants captured between 2019 and 2021 on a small island in the center of the Bohai Strait, which serves as a seasonal migration pathway for this pest species, and 16.1% of the tested moths exhibited pollen contamination, primarily on the proboscis. Subsequently, 33 taxa from at least 23 plant families and 29 genera were identified using a combination of DNA barcoding and pollen morphology, primarily from the Angiosperm, Dicotyledoneae. Moreover, the sex, inter-annual, and seasonal differences in pollen adherence ratio and pollen taxa were revealed. Notably, compared to previously reported pollen types found on several other nocturnal moths, we found that almost all of the above 33 pollen taxa can be found in multiple nocturnal moth species, providing another important example of conspecific attraction. Additionally, we also discussed the indicative significance of the pollen present on the bodies of migratory individuals for determining their migratory route. Overall, by delineating the adult feeding and pollination behavior of S. exigua, we advanced our understanding of the interactions of the moths with their host plants, and its migration pattern, as well as facilitated the design of (area-wide) management strategies to preserve and optimize ecosystem services that they provide.
New Roles for Old Friends: Involvement of the Innate Immune System in Tumor Progression
Authors: Castaño María , González-Cantó Eva , Aghababyan Cristina , Tomás-Pérez Sarai , Oto Julia , Herranz Raquel , Medina Pilar , Götte Martin , Mc Cormack Bárbara Andrea , Marí-Alexandre Josep , Gilabert-Estellés Juan
Published: April 2023

The association between the immune system and tumor progression has attracted much interest in the research community in recent years [...]
Multifunctional Nanomaterials: Synthesis, Properties, and Applications 2.0
Authors: Yadav Raghvendra Singh
Published: April 2023

This Special Issue, “Multifunctional Nanomaterials: Synthesis, Properties and Applications 2 [...]
Polylactic-Containing Hyperbranched Polymers through the CuAAC Polymerization of Aromatic AB2 Monomers
Authors: Pacini Aurora , Nitti Andrea , Vitale Marcello , Pasini Dario
Published: April 2023

We report on the synthesis and characterization of a novel class of hyperbranched polymers, in which a copper(I)-catalyzed alkyne azide cycloaddition (CuAAC) reaction (the prototypical “click” reaction) is used as the polymerization step. The AB2 monomers bear two azide functionalities and one alkyne functionality, which have been installed onto a 1,3,5 trisubstituted benzene aromatic skeleton. This synthesis has been optimized in terms of its purification strategies, with an eye on its scalability for the potential industrial applications of hyperbranched polymers as viscosity modifiers. By taking advantage of the modularity of the synthesis, we have been able to install short polylactic acid fragments as the spacing units between the complementary reactive azide and alkyne functionalities, aiming to introduce elements of biodegradability into the final products. The hyperbranched polymers have been obtained with good molecular weights and degrees of polymerization and branching, testifying to the effectiveness of the synthetic design. Simple experiments on glass surfaces have highlighted the possibility of conducting the polymerizations and the formation of the hyperbranched polymers directly in thin films at room temperature.
Chromium Complexes Supported by Salen-Type Ligands for the Synthesis of Polyesters, Polycarbonates, and Their Copolymers through Chemoselective Catalysis
Authors: Grimaldi Ilaria , Santulli Federica , Lamberti Marina , Mazzeo Mina
Published: April 2023

Salen, Salan, and Salalen chromium (III) chloride complexes have been investigated as catalysts for the ring-opening copolymerization reactions of cyclohexene oxide (CHO) with CO2 and of phthalic anhydride (PA) with limonene oxide (LO) or cyclohexene oxide (CHO). In the production of polycarbonates, the more flexible skeleton of salalen and salan ancillary ligands favors high activity. Differently, in the copolymerization of phthalic anhydride with the epoxides, the salen complex showed the best performance. Diblock polycarbonate-polyester copolymers were selectively obtained by one-pot procedures from mixtures of CO2, cyclohexene oxide, and phthalic anhydride with all complexes. In addition, all chromium complexes were revealed to be very active in the chemical depolymerization of polycyclohexene carbonate producing cyclohexene oxide with high selectivity, thus offering the opportunity to close the loop on the life of these materials.
Preparation and Properties of (Sc2O3-MgO)/Pcl/Pvp Electrospun Nanofiber Membranes for the Inhibition of Escherichia coli Infections
Authors: Liu Yanjing , Li Xiyue , Liu Yuezhou , Huang Yaping , Wang Fuming , Qian Yongfang , Wang Ying
Published: April 2023

Due to their high porosity, large specific surface area, and structural similarity with the extracellular matrix (ECM), electrospun nanofiber membranes are often endowed with the antibacterial properties for biomedical applications. The purpose of this study was to synthesize nano-structured Sc2O3-MgO by doping Sc3+, calcining at 600 °C, and then loading it onto the PCL/PVP substrates with electrospinning technology with the aim of developing new efficient antibacterial nanofiber membranes for tissue engineering. A scanning electron microscope (SEM) and energy dispersive X-ray spectrometer (EDS) were used to study the morphology of all formulations and analyze the types and contents of the elements, and an X-ray diffraction (XRD), thermogravimetric analysis (TGA), and Fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR) were used for further analysis. The experimental results showed that the PCL/PVP (SMCV-2.0) nanofibers loaded with 2.0 wt% Sc2O3-MgO were smooth and homogeneous with an average diameter of 252.6 nm; the antibacterial test indicated that a low load concentration of 2.0 wt% Sc2O3-MgO in PCL/PVP (SMCV-2.0) showed a 100% antibacterial rate against Escherichia coli (E. coli).
The Novel Role of Noncoding RNAs in Modulating Platelet Function: Implications in Activation and Aggregation
Authors: Cimmino Giovanni , Conte Stefano , Palumbo Domenico , Sperlongano Simona , Torella Michele , Della Corte Alessandro , Golino Paolo
Published: April 2023

It is currently believed that plaque complication, with the consequent superimposed thrombosis, is a key factor in the clinical occurrence of acute coronary syndromes (ACSs). Platelets are major players in this process. Despite the considerable progress made by the new antithrombotic strategies (P2Y12 receptor inhibitors, new oral anticoagulants, thrombin direct inhibitors, etc.) in terms of a reduction in major cardiovascular events, a significant number of patients with previous ACSs treated with these drugs continue to experience events, indicating that the mechanisms of platelet remain largely unknown. In the last decade, our knowledge of platelet pathophysiology has improved. It has been reported that, in response to physiological and pathological stimuli, platelet activation is accompanied by de novo protein synthesis, through a rapid and particularly well-regulated translation of resident mRNAs of megakaryocytic derivation. Although the platelets are anucleate, they indeed contain an important fraction of mRNAs that can be quickly used for protein synthesis following their activation. A better understanding of the pathophysiology of platelet activation and the interaction with the main cellular components of the vascular wall will open up new perspectives in the treatment of the majority of thrombotic disorders, such as ACSs, stroke, and peripheral artery diseases before and after the acute event. In the present review, we will discuss the novel role of noncoding RNAs in modulating platelet function, highlighting the possible implications in activation and aggregation.
Methods to Identify Cognitive Alterations from Animals to Humans: A Translational Approach
Authors: Navarro Daniela , Gasparyan Ani , Martí Martínez Silvia , Díaz Marín Carmen , Navarrete Francisco , García Gutiérrez María Salud , Manzanares Jorge
Published: April 2023

The increasing prevalence of cognitive dysfunction and dementia in developed countries, associated with population aging, has generated great interest in characterizing and quantifying cognitive deficits in these patients. An essential tool for accurate diagnosis is cognitive assessment, a lengthy process that depends on the cognitive domains analyzed. Cognitive tests, functional capacity scales, and advanced neuroimaging studies explore the different mental functions in clinical practice. On the other hand, animal models of human diseases with cognitive impairment are essential for understanding disease pathophysiology. The study of cognitive function using animal models encompasses multiple dimensions, and deciding which ones to investigate is necessary to select the most appropriate and specific tests. Therefore, this review studies the main cognitive tests for assessing cognitive deficits in patients with neurodegenerative diseases. Cognitive tests, the most commonly used functional capacity scales, and those resulting from previous evidence are considered. In addition, the leading behavioral tests that assess cognitive functions in animal models of disorders with cognitive impairment are highlighted.
Drug Discovery Targeting Post-Translational Modifications in Response to DNA Damages Induced by Space Radiation
Authors: Xie Dafei , Huang Qi , Zhou Pingkun
Published: April 2023

DNA damage in astronauts induced by cosmic radiation poses a major barrier to human space exploration. Cellular responses and repair of the most lethal DNA double-strand breaks (DSBs) are crucial for genomic integrity and cell survival. Post-translational modifications (PTMs), including phosphorylation, ubiquitylation, and SUMOylation, are among the regulatory factors modulating a delicate balance and choice between predominant DSB repair pathways, such as non-homologous end joining (NHEJ) and homologous recombination (HR). In this review, we focused on the engagement of proteins in the DNA damage response (DDR) modulated by phosphorylation and ubiquitylation, including ATM, DNA-PKcs, CtIP, MDM2, and ubiquitin ligases. The involvement and function of acetylation, methylation, PARylation, and their essential proteins were also investigated, providing a repository of candidate targets for DDR regulators. However, there is a lack of radioprotectors in spite of their consideration in the discovery of radiosensitizers. We proposed new perspectives for the research and development of future agents against space radiation by the systematic integration and utilization of evolutionary strategies, including multi-omics analyses, rational computing methods, drug repositioning, and combinations of drugs and targets, which may facilitate the use of radioprotectors in practical applications in human space exploration to combat fatal radiation hazards.
Inflammatory Response and Exosome Biogenesis of Choroid Plexus Organoids Derived from Human Pluripotent Stem Cells
Authors: Muok Laureana , Liu Chang , Chen Xingchi , Esmonde Colin , Arthur Peggy , Wang Xueju , Singh Mandip , Driscoll Tristan , Li Yan
Published: April 2023

The choroid plexus (ChP) is a complex structure in the human brain that is responsible for the secretion of cerebrospinal fluid (CSF) and forming the blood–CSF barrier (B-CSF-B). Human-induced pluripotent stem cells (hiPSCs) have shown promising results in the formation of brain organoids in vitro; however, very few studies to date have generated ChP organoids. In particular, no study has assessed the inflammatory response and the extracellular vesicle (EV) biogenesis of hiPSC-derived ChP organoids. In this study, the impacts of Wnt signaling on the inflammatory response and EV biogenesis of ChP organoids derived from hiPSCs was investigated. During days 10–15, bone morphogenetic protein 4 was added along with (+/−) CHIR99021 (CHIR, a small molecule GSK-3β inhibitor that acts as a Wnt agonist). At day 30, the ChP organoids were characterized by immunocytochemistry and flow cytometry for TTR (~72%) and CLIC6 (~20%) expression. Compared to the −CHIR group, the +CHIR group showed an upregulation of 6 out of 10 tested ChP genes, including CLIC6 (2-fold), PLEC (4-fold), PLTP (2–4-fold), DCN (~7-fold), DLK1 (2–4-fold), and AQP1 (1.4-fold), and a downregulation of TTR (0.1-fold), IGFBP7 (0.8-fold), MSX1 (0.4-fold), and LUM (0.2–0.4-fold). When exposed to amyloid beta 42 oligomers, the +CHIR group had a more sensitive response as evidenced by the upregulation of inflammation-related genes such as TNFα, IL-6, and MMP2/9 when compared to the −CHIR group. Developmentally, the EV biogenesis markers of ChP organoids showed an increase over time from day 19 to day 38. This study is significant in that it provides a model of the human B-CSF-B and ChP tissue for the purpose of drug screening and designing drug delivery systems to treat neurological disorders such as Alzheimer’s disease and ischemic stroke.
Oscillation of Autophagy Induction under Cellular Stress and What Lies behind It, a Systems Biology Study
Authors: Hajdú Bence , Csabai Luca , Márton Margita , Holczer Marianna , Korcsmáros Tamás , Kapuy Orsolya
Published: April 2023

One of the main inducers of autophagy-dependent self-cannibalism, called ULK1, is tightly regulated by the two sensor molecules of nutrient conditions and energy status, known as mTOR and AMPK kinases, respectively. Recently, we developed a freely available mathematical model to explore the oscillatory characteristic of the AMPK-mTOR-ULK1 regulatory triangle. Here, we introduce a systems biology analysis to explain in detail the dynamical features of the essential negative and double-negative feedback loops and also the periodic repeat of autophagy induction upon cellular stress. We propose an additional regulatory molecule in the autophagy control network that delays some of AMPK’s effect on the system, making the model output more consistent with experimental results. Furthermore, a network analysis on AutophagyNet was carried out to identify which proteins could be the proposed regulatory components in the system. These regulatory proteins should satisfy the following rules: (1) they are induced by AMPK; (2) they promote ULK1; (3) they down-regulate mTOR upon cellular stress. We have found 16 such regulatory components that have been experimentally proven to satisfy at least two of the given rules. Identifying such critical regulators of autophagy induction could support anti-cancer- and ageing-related therapeutic efforts.
The Impact of YouTube on Loneliness and Mental Health
Authors: Balcombe Luke , De Leo Diego
Published: April 2023

There are positives and negatives of using YouTube in terms of loneliness and mental health. YouTube’s streaming content is an amazing resource, however, there may be bias or errors in its recommendation algorithms. Parasocial relationships can also complicate the impact of YouTube use. Intervention may be necessary when problematic and risky content is associated with unhealthy behaviors and negative impacts on mental health. Children and adolescents are particularly vulnerable. Although YouTube might assist in connecting with peers, there are privacy, safety, and quality issues to consider. This paper is an integrative review of the positive and negative impacts of YouTube with the aim to inform the design and development of a technology-based intervention to improve mental health. The impact of YouTube use on loneliness and mental health was explored by synthesizing a purposive selection (n = 32) of the empirical and theoretical literature. Next, we explored human–computer interaction issues and proposed a concept whereby an independent-of-YouTube algorithmic recommendation system steers users toward verified positive mental health content or promotions.
The Effects of Biological Age on Speed-Explosive Properties in Young Tennis Players
Authors: Sinkovic Filip , Novak Dario , Foretic Nikola , Zemková Erika
Published: April 2023

Biological maturity can affect performance on motor tests, thus young players can have advantages or disadvantages during testing by being more or less mature than their peers of the same chronological age. The aim of this study was to investigate the effects of biological age on speed, agility, and explosive power in young tennis players. Fifty tennis players (age 12.3 ± 1.2 years, height 156.7 ± 12.8 cm, body mass 45.9 ± 8.9 kg) who were ranked up to 50th place in the ranking of the National Tennis Association, as well as up to 300th place on the international “Tennis Europe” ranking, participated in the study. They were divided into three groups according to their maturation status, that is, the peak height velocity (PHV) maturity offset: pre-PHV [<0] (n = 10); circa-PHV [0 to 1] (n = 10); and post-PHV [>1.5] (n = 25). They performed tests of speed (5, 10, and 20 m sprints), agility (20 yards, 4 × 10 yards, T-test, TENCODS, and TENRAG), and explosive power (countermovement jump, one-leg countermovement jump, squat jump, long jump, and one-leg triple jump). Results showed significantly higher height of the vertical jump in the post-PHV group compared to the pre-PHV group, in the range of ~16% to ~27%. Moreover, linear and change of direction speed was significantly faster in the post-PHV group compared to the pre-PHV group, in the range of ~5% to ~8%. Height of the squat jump and speed in the T-test were significantly better in the post-PHV group compared to the circa-PHV group, in the range of ~7% to ~15%, while height of the single-leg triple jump was significantly higher in the circa-PHV group compared to the pre-PHV group by ~7%. This study showed that tennis players of older biological age achieve better results in almost all variables of speed, agility, and explosive power compared to players of younger biological age. Coaches should be aware of the differences found in physical performance and consider the practical implications that maturation can have in the long-term development of young tennis players.
Tell Us What You Really Think: A Think Aloud Protocol Analysis of the Verbal Cognitive Reflection Test
Authors: Byrd Nick , Joseph Brianna , Gongora Gabriela , Sirota Miroslav
Published: April 2023

The standard interpretation of cognitive reflection tests assumes that correct responses are reflective and lured responses are unreflective. However, prior process-tracing of mathematical reflection tests has cast doubt on this interpretation. In two studies (N = 201), we deployed a validated think-aloud protocol in-person and online to test how this assumption is satisfied by the new, validated, less familiar, and non-mathematical verbal Cognitive Reflection Test (vCRT). Verbalized thoughts in both studies revealed that most (but not all) correct responses involved reflection and that most (but not all) lured responses lacked reflection. The think-aloud protocols seemed to reflect business-as-usual performance: thinking aloud did not disrupt test performance compared to a control group. These data suggest that the vCRT usually satisfies the standard interpretation of the reflection tests (albeit not without exceptions) and that the vCRT can be a good measure of the construct theorized by the two-factor explication of ‘reflection’ (as deliberate and conscious).
TRFM-LS: Transformer-Based Deep Learning Method for Vessel Trajectory Prediction
Authors: Jiang Dapeng , Shi Guoyou , Li Na , Ma Lin , Li Weifeng , Shi Jiahui
Published: April 2023

In the context of the rapid development of deep learning theory, predicting future motion states based on time series sequence data of ship trajectories can significantly improve the safety of the traffic environment. Considering the spatiotemporal correlation of AIS data, a trajectory time window panning and smoothing filtering method is proposed for the abnormal values existing in the trajectory data. The application of this method can effectively deal with the jump values and outliers in the trajectory data, make the trajectory smooth and continuous, and ensure the temporal order and integrity of the trajectory data. In this paper, for the features of spatiotemporal data of trajectories, the LSTM structure is integrated on the basis of the deep learning Transformer algorithm framework, abbreviated as TRFM-LS. The LSTM module can learn the temporal features of spatiotemporal data in the process of computing the target sequence, while the self-attention mechanism in Transformer can solve the drawback of applying LSTM to capture the sequence information weakly at a distance. The advantage of complementarity of the fusion model in the training process of trajectory sequences with respect to the long-range dependence of temporal and spatial features is realized. Finally, in the comparative analysis section of the error metrics, by comparing with current state-of-the-art methods, the algorithm in this paper is shown to have higher accuracy in predicting time series trajectory data. The research in this paper provides an early warning information reference for autonomous navigation and autonomous collision avoidance of ships in practice.
Three-Dimensional-Printed Coral-like Structures as a Habitat for Reef Fish
Authors: Oren Asa , Berman Ofer , Neri Reem , Tarazi Ezri , Parnas Haim , Lotan Offri , Zoabi Majeed , Josef Noam , Shashar Nadav
Published: April 2023

Coral reefs are three-dimensional biogenic structures that provide habitat for plenty of marine organisms; yet, coral reefs are deteriorating worldwide. Hence, it is essential to identify suitable substitutes for such coral services. This study examines reef fishes’ behavior and reactions to three-dimensional-printed (3DP) corals based on scanned Stylophora pistillata, as well as modified 3DP models. In particular, fishes’ unresponsiveness to the color, shape, morphology, and material of 3DP models both in vitro and in situ experiments was investigated. Coral reef fishes responded to the 3DP corals and demonstrated their usage in a range of services. Moreover, a greater number of fish species interacted more with 3DP models than they did with live corals. Furthermore, specific reef fish species, such as Sea Goldies (Pseudanthias squamipinnis), showed a preference for specific 3DP coral color, and other species demonstrated preferences for specific 3DP model shapes. The current study results show that three-dimensional-printed coral models can substitute for live corals for certain types of reef fish services.
AoMae1 Regulates Hyphal Fusion, Lipid Droplet Accumulation, Conidiation, and Trap Formation in Arthrobotrys oligospora
Authors: Liu Yankun , Zhu Meichen , Wang Wenjie , Li Xuemei , Bai Na , Xie Meihua , Yang Jinkui
Published: April 2023

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid (TCA) cycle and is essential for energy balance, growth, and tolerance to cold and salt stresses in plants. However, the role of MDH in filamentous fungi is still largely unknown. In this study, we characterized an ortholog of MDH (AoMae1) in a representative nematode-trapping (NT) fungus Arthrobotrys oligospora via gene disruption, phenotypic analysis, and nontargeted metabolomics. We found that the loss of Aomae1 led to a weakening of MDH activity and ATP content, a remarkable decrease in conidia yield, and a considerable increase in the number of traps and mycelial loops. In addition, the absence of Aomae1 also caused an obvious reduction in the number of septa and nuclei. In particular, AoMae1 regulates hyphal fusion under low nutrient conditions but not in nutrient-rich conditions, and the volumes and sizes of the lipid droplets dynamically changed during trap formation and nematode predation. AoMae1 is also involved in the regulation of secondary metabolites such as arthrobotrisins. These results suggest that Aomae1 has an important role in hyphal fusion, sporulation, energy production, trap formation, and pathogenicity in A. oligospora. Our results enhance the understanding of the crucial role that enzymes involved in the TCA cycle play in the growth, development, and pathogenicity of NT fungi.
Beech Leaf Disease Severity Affects Ectomycorrhizal Colonization and Fungal Taxa Composition
Authors: Bashian-Victoroff Claudia , Brown Alexis , Loyd Andrew L. , Carrino-Kyker Sarah R. , Burke David J.
Published: April 2023

Beech leaf disease (BLD) is an emerging forest infestation affecting beech trees (Fagus spp.) in the midwestern and northeastern United States and southeastern Canada. BLD is attributed to the newly recognized nematode Litylenchus crenatae subsp. mccannii. First described in Lake County, Ohio, BLD leads to the disfigurement of leaves, canopy loss, and eventual tree mortality. Canopy loss limits photosynthetic capacity, likely impacting tree allocation to belowground carbon storage. Ectomycorrhizal fungi are root symbionts, which rely on the photosynthesis of autotrophs for nutrition and growth. Because BLD limits tree photosynthetic capacity, ECM fungi may receive less carbohydrates when associating with severely affected trees compared with trees without BLD symptoms. We sampled root fragments from cultivated F. grandifolia sourced from two provenances (Michigan and Maine) at two timepoints (fall 2020 and spring 2021) to test whether BLD symptom severity alters colonization by ectomycorrhizal fungi and fungal community composition. The studied trees are part of a long-term beech bark disease resistance plantation at the Holden Arboretum. We sampled from replicates across three levels of BLD symptom severity and compared fungal colonization via visual scoring of ectomycorrhizal root tip abundance. Effects of BLD on fungal communities were determined through high-throughput sequencing. We found that ectomycorrhizal root tip abundance was significantly reduced on the roots of individuals of the poor canopy condition resulting from BLD, but only in the fall 2020 collection. We found significantly more ectomycorrhizal root tips from root fragments collected in fall 2020 than in spring 2021, suggesting a seasonal effect. Community composition of ectomycorrhizal fungi was not impacted by tree condition but did vary between provenances. We found significant species level responses of ectomycorrhizal fungi between levels of both provenance and tree condition. Of the taxa analyzed, two zOTUs had significantly lower abundance in high-symptomatology trees compared with low-symptomatology trees. These results provide the first indication of a belowground effect of BLD on ectomycorrhizal fungi and contribute further evidence to the role of these root symbionts in studies of tree disease and forest pathology.
Thermo-Hydrodynamic Lubricating Behaviors of Upstream Liquid Face Seals with Ellipse Dimples
Authors: Bai Shaoxian , Li Kaixin , Yang Jing , Bao Shiyi , Ma Chunhong
Published: April 2023

In order to obtain the leakage characteristics of an upstream pumping face seal with inclined ellipse dimples under high-temperature and high-speed liquid lubricating conditions, a thermo-hydrodynamic lubricating model is developed. The novelty of this model is that it takes the thermo-viscosity effect and cavitation effect into account. The influence of operating parameters, such as rotational speed, seal clearance, seal pressure, ambient temperature and structural parameters, such as dimple depth, inclination angle, slender ratio and dimple number on the opening force and leakage rate, is numerically calculated. The results obtained show that the thermo-viscosity effect makes the cavitation intensity decrease noticeably, leading to an increase in the upstream pumping effect of ellipse dimples. Moreover, the thermo-viscosity effect may make both the upstream pumping leakage rate and opening force increase by about 10%. It can also be found that the inclined ellipse dimples can produce an obvious upstream pumping effect and hydrodynamic effect. Based on the reasonable design of the dimple parameter, not only can the sealed medium achieve zero leakage, but the opening force can also increase by more than 50%. The proposed model has the potential to provide the theoretical basis for and guide the future designs of upstreaming liquid face seals.
Research Progress of Laser Cladding on the Surface of Titanium and Its Alloys
Authors: Zhao Hui , Zhao Chaochao , Xie Weixin , Wu Di , Du Beining , Zhang Xingru , Wen Min , Ma Rui , Li Rui , Jiao Junke , Chang Cheng , Yan Xingchen , Sheng Liyuan
Published: April 2023

Titanium (Ti) and its alloys have been widely employed in aeronautical, petrochemical, and medical fields owing to their fascinating advantages in terms of their mechanical properties, corrosion resistance, biocompatibility, and so on. However, Ti and its alloys face many challenges, if they work in severe or more complex environments. The surface is always the origin of failure for Ti and its alloys in workpieces, which influences performance degradation and service life. To improve the properties and function, surface modification becomes the common process for Ti and its alloys. The present article reviews the technology and development of laser cladding on Ti and its alloys, according to the cladding technology, cladding materials, and coating function. Generally, the laser cladding parameters and auxiliary technology could influence the temperature distribution and elements diffusion in the molten pool, which basically determines the microstructure and properties. The matrix and reinforced phases play an important role in laser cladding coating, which can increase the hardness, strength, wear resistance, oxidation resistance, corrosion resistance, biocompatibility, and so on. However, the excessive addition of reinforced phases or particles can deteriorate the ductility, and thus the balance between functional properties and basic properties should be considered during the design of the chemical composition of laser cladding coatings. In addition, the interface including the phase interface, layer interface, and substrate interface plays an important role in microstructure stability, thermal stability, chemical stability, and mechanical reliability. Therefore, the substrate state, the chemical composition of the laser cladding coating and substrate, the processing parameters, and the interface comprise the critical factors which influence the microstructure and properties of the laser cladding coating prepared. How to systematically optimize the influencing factors and obtain well-balanced performance are long-term research issues.
Derivation of Corrosion Depth Formula According to Corrosion Factors in District Heating Water through Regression Analysis
Authors: So Yoon-Sik , Lim Jeong-Min , Kang Sin-Jae , Kim Woo-Cheol , Kim Jung-Gu
Published: April 2023

In order to predict the corrosion depth of a district heating pipeline, it is necessary to analyze various corrosion factors. In this study, the relationship between corrosion factors such as pH, dissolved oxygen, and operating time and corrosion depth was investigated using the Box–Behnken method within the response surface methodology. To accelerate the corrosion process, galvanostatic tests were conducted in synthetic district heating water. Subsequently, a multiple regression analysis was performed using the measured corrosion depth to derive a formula for predicting the corrosion depth as a function of the corrosion factors. As a result, the following regression formula was derived for predicting the corrosion depth: “corrosion depth (μm) = −133 + 17.1 pH + 0.00072 DO + 125.2 Time − 7.95 pH × Time + 0.002921 DO × Time”.
Single-Shot Multi-Frame Imaging of Femtosecond Laser-Induced Plasma Propagation
Authors: Zhang Tianyong , Guo Baoshan , Jiang Lan , Zhu Tong , Hua Yanhong , Zhan Ningwei , Yao Huan
Published: April 2023

Single-shot ultrafast multi-frame imaging technology plays a crucial role in the observation of laser-induced plasma. However, there are many challenges in the application of laser processing, such as technology fusion and imaging stability. To provide a stable and reliable observation method, we propose an ultrafast single-shot multi-frame imaging technology based on wavelength polarization multiplexing. Through the frequency doubling and birefringence effects of the BBO and the quartz crystal, the 800 nm femtosecond laser pulse was frequency doubled to 400 nm, and a sequence of probe sub-pulses with dual-wavelength and different polarization was generated. The coaxial propagation and framing imaging of multi-frequency pulses provided stable imaging quality and clarity, as well as high temporal/spatial resolution (200 fs and 228 lp/mm). In the experiments involving femtosecond laser-induced plasma propagation, the probe sub-pulses measured their time intervals by capturing the same results. Specifically, the measured time intervals were 200 fs between the same color pulses and 1 ps between the adjacent different. Finally, based on the obtained system time resolution, we observed and revealed the evolution mechanism of femtosecond laser-induced air plasma filaments, the multifilament propagation of femtosecond laser in fused silica, and the influence mechanism of air ionization on laser-induced shock waves.
Modeling and Testing of a Composite Steel–Concrete Joint for Hybrid Girder Bridges
Authors: Shangguan Bing , Su Qingtian , Casas Joan R. , Su Hang , Wang Shengyun , Zhao Rongxin
Published: April 2023

A hybrid girder bridge adopts a steel segment at the mid-span of the main span of a continuous concrete girder bridge. The critical point of the hybrid solution is the transition zone, connecting the steel and concrete segments of the beam. Although many girder tests revealing the structural behavior of hybrid girders have been conducted by previous studies, few specimens took the full section of a steel–concrete joint due to the large size of prototype hybrid bridges. In this study, a static load test on a composite segment to bridge the joint between the concrete and steel parts of a hybrid bridge with full section was conducted. A finite element model replicating the tested specimen results was established through Abaqus, while parametric studies were also conducted. The test and numerical results revealed that the concrete filling in the composite solution prevented the steel flange from extensive buckling, which significantly improved the load-carrying capacity of the steel–concrete joint. Meanwhile, strengthening the interaction between the steel and concrete helps to prevent the interlayer slip and simultaneously contributes to a higher flexural stiffness. These results are an important basis for establishing a rational design scheme for the steel–concrete joint of hybrid girder bridges.