bluuebunny/crossref_metadata_2025 huggingface dataset:
A Panel of Urinary Volatile Biomarkers for Differential Diagnosis of Prostate Cancer from Other Urological Cancers
Authors: Lima Ana Rita , Pinto Joana , Carvalho-Maia Carina , Jerónimo Carmen , Henrique Rui , Bastos Maria de Lourdes , Carvalho Márcia , Guedes de Pinho Paula
Published: July 2020

Our group recently developed a urinary 6-biomarker panel for the diagnosis of prostate cancer (PCa) which has a higher level of accuracy compared to the serum prostate specific antigen (PSA) test. Herein, urine from an independent cohort of PCa patients and cancer-free controls was analyzed to further validate the discriminative power of that panel. Additionally, urine from patients diagnosed with bladder cancer (BC) and renal cancer (RC) were included to evaluate the site-specificity of the panel. Results confirmed the ability of the 6-biomarker panel to discriminate PCa patients from controls, but not from other urological cancers. To overcome this limitation, an untargeted approach was performed to unveil discriminant metabolites among the three cancer types. A 10-biomarker panel comprising the original panel plus four new metabolites was established to discriminate PCa from controls, BC, and RC, with 76% sensitivity, 90% specificity, and 92% accuracy. This improved panel also disclosed better accuracy than serum PSA test and provides the basis for a new non-invasive early detection tool for PCa.
Prognostic Value of Immune Environment Analysis in Small Bowel Adenocarcinomas with Verified Mutational Landscape and Predisposing Conditions
Authors: Wirta Erkki-Ville , Szeto Säde , Hänninen Ulrika , Ahtiainen Maarit , Böhm Jan , Mecklin Jukka-Pekka , Aaltonen Lauri A. , Seppälä Toni T.
Published: July 2020

Background: Small bowel adenocarcinoma (SBA) is a rare yet insidious cancer with poor survival. The abundance of tumour-infiltrating lymphocytes is associated with improved survival, but the role of the programmed death-1/programmed death ligand-1 (PD-1/PD-L1) pathway in tumour escape is controversial. We evaluated immune cell infiltration, PD1/PD-L1 expression and their prognostic value in a series of SBAs with previously verified predisposing conditions and exome-wide somatic mutation characterization. Methods: Formalin-fixed paraffin-embedded tissue sections stained for CD3, CD8, PD-L1 and PD-1 were analysed from 94 SBAs. An immune cell score (ICS) was formed from the amount of the CD3 and CD8 positive lymphocytes from the tumour centre and invasive margin. The PD-L1 and PD-1 positive immune cells (ICs) and ICS were combined into a variable called Immunoprofile. Results: High ICS, PD-L1IC and PD-1, individually and combined as Immunoprofile, were prognostic for better patient outcome. Sixty-five (69%) SBAs expressed ≥1% positive PD-L1IC. A high tumour mutation burden was common (19%) and associated with immune markers. Immunoprofile, adjusted for TNM stage, mismatch repair status, tumour location, sex and age were independent prognostic markers for disease-specific and overall survival. Conclusions: Analysing tumoral immune contexture provides prognostic information in SBA. Combining ICS, PD-1 and PD-L1IC as Immunoprofile enhanced the prognostic performance.
Conversion of Xylose to Furfural over Lignin-Based Activated Carbon-Supported Iron Catalysts
Authors: Rusanen Annu , Kupila Riikka , Lappalainen Katja , Kärkkäinen Johanna , Hu Tao , Lassi Ulla
Published: July 2020

In this study, conversion of xylose to furfural was studied using lignin-based activated carbon-supported iron catalysts. First, three activated carbon supports were prepared from hydrolysis lignin with different activation methods. The supports were modified with different metal precursors and metal concentrations into five iron catalysts. The prepared catalysts were studied in furfural production from xylose using different reaction temperatures and times. The best results were achieved with a 4 wt% iron-containing catalyst, 5Fe-ACs, which produced a 57% furfural yield, 92% xylose conversion and 65% reaction selectivity at 170 °C in 3 h. The amount of Fe in 5Fe-ACs was only 3.6 µmol and using this amount of homogeneous FeCl3 as a catalyst, reduced the furfural yield, xylose conversion and selectivity. Good catalytic activity of 5Fe-ACs could be associated with iron oxide and hydroxyl groups on the catalyst surface. Based on the recycling experiments, the prepared catalyst needs some improvements to increase its stability but it is a feasible alternative to homogeneous FeCl3.
RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition
Authors: Morin Guillaume , Pinel Karine , Dias Karine , Seiliez Iban , Beaumatin Florian
Published: July 2020

Nowadays, aquaculture provides more than 50% of fish consumed worldwide but faces new issues that challenge its sustainability. One of them relies on the replacement of fish meal (FM) in aquaculture feeds by other protein sources without deeply affecting the whole organism’s homeostasis. Multiple strategies have already been tested using in vivo approaches, but they hardly managed to cope with the multifactorial problems related to the complexities of fish biology together with new feed formulations. In this context, rainbow trout (RT) is particularly concerned by these problems, since, as a carnivorous fish, dietary proteins provide the amino acids required to supply most of its energetic metabolism. Surprisingly, we noticed that in vitro approaches considering RT cell lines as models to study RT amino acid metabolism were never previously used. Therefore, we decided to investigate if, and how, three major pathways described, in other species, to be regulated by amino acid and to control cellular homeostasis were functional in a RT cell line called RTH-149—namely, the mechanistic Target Of Rapamycin (mTOR), autophagy and the general control nonderepressible 2 (GCN2) pathways. Our results not only demonstrated that these three pathways were functional in RTH-149 cells, but they also highlighted some RT specificities with respect to the time response, amino acid dependencies and the activation levels of their downstream targets. Altogether, this article demonstrated, for the first time, that RT cell lines could represent an interesting alternative of in vivo experimentations for the study of fish nutrition-related questions.
Marine Actinomycetes-Derived Secondary Metabolites Overcome TRAIL-Resistance via the Intrinsic Pathway through Downregulation of Survivin and XIAP
Authors: Elmallah Mohammed I. Y. , Cogo Sheron , Constantinescu Andrei A. , Elifio-Esposito Selene , Abdelfattah Mohammed S. , Micheau Olivier
Published: July 2020

Resistance of cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis represents the major hurdle to the clinical use of TRAIL or its derivatives. The discovery and development of lead compounds able to sensitize tumor cells to TRAIL-induced cell death is thus likely to overcome this limitation. We recently reported that marine actinomycetes’ crude extracts could restore TRAIL sensitivity of the MDA-MB-231 resistant triple negative breast cancer cell line. We demonstrate in this study, that purified secondary metabolites originating from distinct marine actinomycetes (sharkquinone (1), resistomycin (2), undecylprodigiosin (3), butylcyclopentylprodigiosin (4), elloxizanone A (5) and B (6), carboxyexfoliazone (7), and exfoliazone (8)), alone, and in a concentration-dependent manner, induce killing in both MDA-MB-231 and HCT116 cell lines. Combined with TRAIL, these compounds displayed additive to synergistic apoptotic activity in the Jurkat, HCT116 and MDA-MB-231 cell lines. Mechanistically, these secondary metabolites induced and enhanced procaspase-10, -8, -9 and -3 activation leading to an increase in PARP and lamin A/C cleavage. Apoptosis induced by these compounds was blocked by the pan-caspase inhibitor QvD, but not by a deficiency in caspase-8, FADD or TRAIL agonist receptors. Activation of the intrinsic pathway, on the other hand, is likely to explain both their ability to trigger cell death and to restore sensitivity to TRAIL, as it was evidenced that these compounds could induce the downregulation of XIAP and survivin. Our data further highlight that compounds derived from marine sources may lead to novel anti-cancer drug discovery.
Science between Bioreactors and Space Research—Response to Comments by Joseph J. Bevelacqua et al. on “Dexamethasone Inhibits Spheroid Formation of Thyroid Cancer Cells Exposed to Simulated Microgravity”
Authors: Krüger Marcus , Kopp Sascha , Wehland Markus , Corydon Thomas J. , Grimm Daniela
Published: July 2020

We would like to thank Bevelacqua et al [...]
Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer
Authors: Pecinová Alena , Alán Lukáš , Brázdová Andrea , Vrbacký Marek , Pecina Petr , Drahota Zdeněk , Houštěk Josef , Mráček Tomáš
Published: July 2020

Prostate cancer is one of the most prominent cancers diagnosed in males. Contrasting with other cancer types, glucose utilization is not increased in prostate carcinoma cells as they employ different metabolic adaptations involving mitochondria as a source of energy and intermediates required for rapid cell growth. In this regard, prostate cancer cells were associated with higher activity of mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH), the key rate limiting component of the glycerophosphate shuttle, which connects mitochondrial and cytosolic processes and plays significant role in cellular bioenergetics. Our research focused on the role of mGPDH biogenesis and regulation in prostate cancer compared to healthy cells. We show that the 42 amino acid presequence is cleaved from N-terminus during mGPDH biogenesis. Only the processed form is part of the mGPDH dimer that is the prominent functional enzyme entity. We demonstrate that mGPDH overexpression enhances the wound healing ability in prostate cancer cells. As mGPDH is at the crossroad of glycolysis, lipogenesis and oxidative metabolism, regulation of its activity by intramitochondrial processing might represent rapid means of cellular metabolic adaptations.
Glutaminase Inhibition on NSCLC Depends on Extracellular Alanine Exploitation
Authors: Caiola Elisa , Colombo Marika , Sestito Giovanna , Lupi Monica , Marabese Mirko , Pastorelli Roberta , Broggini Massimo , Brunelli Laura
Published: July 2020

Non-small-cell lung cancer (NSCLC) cell lines vary in their sensitivity to glutaminase inhibitors, so it is important to identify the metabolic assets underling their efficacy in cancer cells. Even though specific genetic lesions such as in KRAS and LKB1 have been associated with reliance on glutamine for their metabolic needs, we found no distinction between glutaminase inhibitor CB-839 sensitivity and resistant phenotypes in NSCLC cells with or without these genetic alterations. We demonstrated the close relationship between environmental alanine uptake and catabolism. This response depended on the individual cell’s ability to employ alanine aminotransferase (GPT2) to compensate the reduced glutamate availability. It may, therefore, be useful to determine GPT2 levels to predict which NSCLC patients would benefit most from glutaminase inhibitor treatment.
Surface Alteration of Borosilicate and Phosphate Nuclear Waste Glasses by Hydration and Irradiation
Authors: Zubekhina Bella Y. , Burakov Boris E. , Ojovan Michael I.
Published: July 2020

We examined the degradation of nuclear waste borosilicate and phosphate glasses containing strong alpha-emitter 238Pu at a specific activity of 6.33 × 105 MBq/g in comparison with similar non-radioactive, non-radioactive irradiated and radioactive samples containing beta- and gamma-emitters, namely radionuclides 134Cs and 137Cs. For irradiation and leaching experiments, we used borosilicate and phosphate glasses, which are well-known and currently used to immobilize high-level radioactive waste. The main focus was the observation of the surface of altered glasses. Comparative analysis of hydrolytic surface alteration of borosilicate and phosphate nuclear waste glasses reveals that the behavior of radioactive samples differs significantly from that of non-radioactive glasses.
MicroRNA: A Key Player for the Interplay of Circadian Rhythm Abnormalities, Sleep Disorders and Neurodegenerative Diseases
Authors: Kinoshita Chisato , Okamoto Yayoi , Aoyama Koji , Nakaki Toshio
Published: July 2020

Circadian rhythms are endogenous 24-h oscillators that regulate the sleep/wake cycles and the timing of biological systems to optimize physiology and behavior for the environmental day/night cycles. The systems are basically generated by transcription–translation feedback loops combined with post-transcriptional and post-translational modification. Recently, evidence is emerging that additional non-coding RNA-based mechanisms are also required to maintain proper clock function. MicroRNA is an especially important factor that plays critical roles in regulating circadian rhythm as well as many other physiological functions. Circadian misalignment not only disturbs the sleep/wake cycle and rhythmic physiological activity but also contributes to the development of various diseases, such as sleep disorders and neurodegenerative diseases. The patient with neurodegenerative diseases often experiences profound disruptions in their circadian rhythms and/or sleep/wake cycles. In addition, a growing body of recent evidence implicates sleep disorders as an early symptom of neurodegenerative diseases, and also suggests that abnormalities in the circadian system lead to the onset and expression of neurodegenerative diseases. The genetic mutations which cause the pathogenesis of familial neurodegenerative diseases have been well studied; however, with the exception of Huntington’s disease, the majority of neurodegenerative diseases are sporadic. Interestingly, the dysfunction of microRNA is increasingly recognized as a cause of sporadic neurodegenerative diseases through the deregulated genes related to the pathogenesis of neurodegenerative disease, some of which are the causative genes of familial neurodegenerative diseases. Here we review the interplay of circadian rhythm disruption, sleep disorders and neurodegenerative disease, and its relation to microRNA, a key regulator of cellular processes.
The Assessment of Finishing Properties on the Mass per Unit Area, Pilling, Bursting Strength, and Wicking Behavior of Polyester Weft-Knitted Jersey Fabric
Authors: Hassan Tufail , Khan Muhammad Qamar , Salam Abdul , Hassan Nafees , Raza Ali , Bukhsh Nabi , Javed Zafar , Kim Ick Soo
Published: July 2020

Finishes bring an alteration to the physical and comfort properties of the textiles. That’s why various finishes are used to impart various functionalities to the fabric surface. However, it may also affect some properties. The purpose of this study is to investigate the effect of various finishes on pilling, mass per unit area, bursting strength, and wicking behavior of the polyester weft-knitted jersey fabric. Herein, 100% spun polyester weft-knitted plain jersey fabric was exposed to different finish treatments to check their effect on the some physical and comfort properties of the fabric like mass per unit area, pilling behavior, bursting strength, and wicking properties of the weft-knitted jersey fabric. The fabric used was knit from 24/1, 100% spun polyester yarn on the single knit circular knitting machine. The developed fabric was washed on Fong machine. Finishes are applied on fabric by “Monofort Stanter” machine. The resultant fabric was characterized by random tumble pilling tester, bursting strength tester, and wicking tester to analyze their pilling grade, bursting strength, and wicking behavior respectively. A significant increase has been found in wicking behavior, mass per unit area, and bursting strength of the fabric after finishing treatments. Moreover, the wicking finish shows the highest reduction in pilling grade from 3.5 to 2.5. Significant improvement has been observed in bursting strength by all finish’s treatment. However, wicking finish treatment results in the highest increase in bursting strength of 4.2%. Significant improvement has been observed in the vertical wicking speed of all treatment except silicon finish which significantly reduces vertical wicking rate. However, the wicking finish (Recipe E) shows the highest increase in wicking rate by 13.75 times as compared to grey fabric.
Capillary-Driven Flow Microfluidics Combined with Smartphone Detection: An Emerging Tool for Point-of-Care Diagnostics
Authors: Hassan Sammer-ul , Tariq Aamira , Noreen Zobia , Donia Ahmed , Zaidi Syed Z. J. , Bokhari Habib , Zhang Xunli
Published: July 2020

Point-of-care (POC) or near-patient testing allows clinicians to accurately achieve real-time diagnostic results performed at or near to the patient site. The outlook of POC devices is to provide quicker analyses that can lead to well-informed clinical decisions and hence improve the health of patients at the point-of-need. Microfluidics plays an important role in the development of POC devices. However, requirements of handling expertise, pumping systems and complex fluidic controls make the technology unaffordable to the current healthcare systems in the world. In recent years, capillary-driven flow microfluidics has emerged as an attractive microfluidic-based technology to overcome these limitations by offering robust, cost-effective and simple-to-operate devices. The internal wall of the microchannels can be pre-coated with reagents, and by merely dipping the device into the patient sample, the sample can be loaded into the microchannel driven by capillary forces and can be detected via handheld or smartphone-based detectors. The capabilities of capillary-driven flow devices have not been fully exploited in developing POC diagnostics, especially for antimicrobial resistance studies in clinical settings. The purpose of this review is to open up this field of microfluidics to the ever-expanding microfluidic-based scientific community.
The Business Side of Ecosystem Services of Soil Systems
Authors: Mikhailova Elena A. , Post Christopher J. , Schlautman Mark A. , Post Gregory C. , Zurqani Hamdi A.
Published: July 2020

Current applications of the Ecosystems Services (ES) framework to soils are narrowly defined (e.g., soil-based, pedosphere-based, etc.), and focus on soil properties while treating soil as a closed system. Because soil is an open system, it receives and loses matter across its boundaries within Earth’s spheres (atmosphere, biosphere, hydrosphere, lithosphere, ecosphere, and anthroposphere), which also need to be accounted for in economic analysis. In market economies, the market transforms resources from the Earth’s pedosphere and related spheres into goods and services for societal welfare with non-market institutions mediating human and environmental interactions. These transformations and mediations can result not only in welfare but damages as well. The concept of soil ES and ecosystem disservices (ED) is a human-centered framework, which can be a useful tool in business decision-making. Soil ES (e.g., provisioning, regulation/ maintenance, and cultural) are used to produce goods and services, but the value of these ES and ED are not always accounted for as a part of business decision-making. The objective of this review is to illustrate the monetary valuation of ecosystem services of soil systems (SS) with examples based on the organizational hierarchy of soil systems. The organizational hierarchy of soil systems can be used in economic valuations of soil ES by scale (e.g., world, continent), time (e.g., soil, geologic), qualitative and quantitative degrees of computation (e.g., mental, verbal, descriptive, mathematical, deterministic, stochastic), and degree of complexity (e.g., mechanistic, empirical). Soil survey databases, soil analyses, Soil Data Systems (SDS), and Soil Business Systems (SBS) provide tools and a wide range of quantitative/qualitative data and information to evaluate goods and services for various business applications, but these sources of soil data may be limited in scope due to their static nature. Valuation of soil resources based on soil and non-soil science databases (e.g., National Atmospheric Deposition Program (NADP) databases, etc.) is critically needed to account for these ES/ED as part of business decision-making to provide more sustainable use of soil resources. Since most ecosystems on Earth have been modified by human activity, “soil systems goods and services” (SSGS) may be a more applicable term to describe soil contributions (benefits/damages) to economic activity, compared to a term such as “soil ecosystem goods and services.”
Leveraging Communities of Practice as Professional Learning Communities in Science, Technology, Engineering, Math (STEM) Education
Authors: Townley Amanda
Published: July 2020

In the modern educational era, there is an increasing focus on Science, Technology, Engineering and Math (STEM) education. Innovation and workforce preparation play a large role in the push to increase scientific literacy, thinking skills and high-skilled personnel. However, there are ongoing issues with reforming education to meet these goals as well as the recruitment and retention of students in these fields. STEM and the various other iterations in which it is found, represents a challenge in education, as there are often many intersecting cultures, epistemologies, practices and expectations that are unique to each of the represented fields. As a result, educators often struggle with creating STEM experiences and programs that truly integrate each of the disciplines in a meaningful way. This discussion explores the potential role of leveraging existing communities of practice into Professional Learning Communities in order to shift the attention toward student experiences while more clearly defining the goals, roles and expectations in STEM.
Both Forest Harvesting and Hydropower Dams Yielded Negative Impact on Low Flow Regimes in the Zagunao River Watershed, Southwest China
Authors: Jiang Zhiwei , Zhang Mingfang , Hou Yiping
Published: July 2020

Forest harvesting and hydropower dams can significantly affect flow regimes (magnitude, timing, duration, frequency, and variability), resulting in changes in degraded aquatic ecosystems and unstable water supply. Despite numerous studies on the effects of forest harvesting on mean flows, the impact of forest harvesting on flow regimes has been less investigated. A great difficulty lies in separating the hydrological effect of forest harvesting from that of climate variability and other watershed disturbances such hydropower dams. In this study, the Upper Zagunao River watershed (2242 km2) was selected as an example to provide a quantitative assessment of the effects of forest harvesting and hydropower dams on low flow regimes. The key findings include: (1) Forest harvesting led to a significant reduction in the magnitude and return period of low flows, and a significant increment in the variability and duration of low flows; (2) the recovery of low flow regimes occurred 40 years after forest harvesting as forest recovery processed; and (3) hydropower dams caused significant impact on all components of low flow regimes, e.g., a reduction in the magnitude, return period, and timing of low flows, and an increment in the variability and duration of low flows. Our findings highlight the negative impact of both forest harvesting and hydropower dams on low flow regimes in the Upper Zagunao River watershed. A watershed management strategy for offsetting the negative effect of hydropower dams on low flow regimes by restoring hydrological functions of subalpine forests is highly recommended in subalpine watersheds of the Upper Yangtze River.
Reconstructing Long Term High Andean Forest Dynamics Using Historical Aerial Imagery: A Case Study in Colombia
Authors: Calbi Mariasole , Clerici Nicola , Borsch Thomas , Brokamp Grischa
Published: July 2020

High Andean forests are biodiversity hotspots that also play key roles in the provisioning of vital ecosystem services for neighboring cities. In past centuries, the hinterland of Andean fast-growing cities often experienced a dramatic decline in forested areas, but there are reports that forest cover has been recovering recently. We analyzed aerial imagery spanning the years 1940 to 2007 from nine administrative localities in the Eastern Andean Cordillera of Colombia in order to elucidate precise patterns of forest vegetation change. To this aim, we performed image object-based classification by means of texture analysis and image segmentation. We then derived connectivity metrics to investigate whether forest cover trajectories showed an increase or decrease in fragmentation and landscape degradation. We observed a forest cover recovery in all the examined localities, except one. In general, forest recovery was accompanied by an increase in core habitat areas. The time scale of the positive trends identified partially coincides with the creation of protected areas in the region, which very likely furthered the recovery of forest patches. This study unveils the long-term dynamics of peri-urban high Andean forest cover, providing valuable information on historical vegetation changes in a highly dynamic landscape.
Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
Authors: Piñuela Yasmine , G. Alday Josu , Oliach Daniel , Bolaño Francesc , Colinas Carlos , Bonet José Antonio
Published: July 2020

Research Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process and the root development of Portuguese oak (Quercus faginea Lam.) seedlings destined for truffle plantations has not been determined. The main aim of this study was to shed light on the effect of bacterial inoculation on fungal root tip colonization and seedling root traits. Material and methods: We performed a co-inoculation trial using three bacteria naturally present in the T. melanosporum niche (i.e., Pseudomonas fluorescens, Pseudomonas putida, and Bacillus amyloliquefaciens) and two different bacterial inoculation times (one month and nine months after fungal inoculation) under glasshouse conditions. Results: Only P. fluorescens had a significant mycorrhizal promoter effect, increasing the truffle inoculation rates of root tips by more than 10% compared with seedlings that received non-bacterial inoculation treatments. Simultaneously, the co-inoculation of P. fluorescens with T. melanosporum improved seedling root growth parameters compared with those of seedlings that received non-bacterial inoculation treatments. The different bacterial inoculation times and applications of uninoculated bacterial growth media did not affect the root traits analyzed or the root mycorrhization rates. Conclusions: These results suggest that P. fluorescens bacteria have a potential commercial application as a treatment for truffle-inoculated seedlings to improve both seedling quality and mycorrhizal colonization under nursery conditions.
An Assessment of Soil’s Nutrient Deficiencies and Their Influence on the Restoration of Degraded Karst Vegetation in Southwest China
Authors: Liu Yuguo , Liu Changcheng , Rubinato Matteo , Guo Ke , Zhou Jinxing , Cui Ming
Published: July 2020

The distribution of karst landscapes over the Earth’s surface, to a large extent, follows the distribution of carbonate (limestone and dolomite) and gypsum rocks and together these make up about 12% of the Earth’s land area, and the largest karst region in to world is in Southwestern China. Characterized by a unique set of landforms, these geographical areas also differ from other geomorphic regions by the presence of cave systems in the subsurface. Unfortunately, due to human disturbances, such as deforestation, agricultural expansion, livestock overgrazing and fire, these regions have been affected by varying degrees of degradation, which could also be worsened if water and soil erosion phenomena typical of these areas are considered. Therefore, there is a need to implement measures and strategies to protect these karst areas and develop plans to restore vegetation in this region. To support local and national authorities to achieve this goal, this study aims to characterize nutrient deficiencies in degraded areas and estimate what could be the thresholds required to facilitate the restoration of vegetation in karst areas in southwest China. The results obtained confirm that the total element concentrations for Soil Organic Carbon (SOC), N, K, Ca, P, S and Mg were relatively high in the study karst area in southwest China. However, the total amounts of soil nutrients stored were very low due to the limited amount of soil identified as a consequence of previous deforestation processes undertaken within this study area and this aspect needs to be taken into consideration if aiming at a positive success of future restoration processes.
Pyropheophytin a in Soft Deodorized Olive Oils
Authors: Gómez-Coca Raquel B. , Alassi Mahmoud , Moreda Wenceslao , Pérez-Camino María del Carmen
Published: July 2020

Mild refined olive oil obtained by neutralization and/or by soft deodorization at a low temperature and its blending with extra virgin olive oil (EVOO) is not allowed and is difficult to detect. Chlorophyll derivatives, pheophytins and pyropheophytin, and their relative proportions were proposed as parameters to detect such processes. The objective of this study is to determine changes in EVOO, in terms of pheophytins and pyropheophytin, occurring after several well-controlled mild refining processes. The changes on those chlorophyll pigments due to the processes depend on the temperature, stripping gas, acidity and oil nature. The data obtained show that, at temperatures below 100 °C, the rate at which pyropheophytin a is formed (Ra) is lower than the rate at which pheophytins a+a’ disappear (Ra+a’). As a consequence, the Ra+a’ and Ra ratios are considered to be directly linked to pheophytins a+a’ decrease instead of to pyropheophytin a formation. Stripping gas very slightly affects the transformation of the chlorophyll pigments; actually both acidity and N2 enhance the increment in the Ra+a’ and Ra ratios. In relation to the oil nature, the higher the initial pheophytin a+a’ content, the higher the increase in the Ra+a’ and Ra relations.
Bee Pollen Role in Red Winemaking: Volatile Compounds and Sensory Characteristics of Tintilla de Rota Warm Climate Red Wines
Authors: Amores-Arrocha Antonio , Sancho-Galán Pau , Jiménez-Cantizano Ana , Palacios Víctor
Published: July 2020

One of the main aspects that define wine quality is its aromatic profile. Nutritional deficiencies in musts can lead to olfactory defects and a decline in quality. Commercial activators and nutrients are usually added to the must in these cases. The natural composition of bee pollen can provide all the necessary nutrients for yeasts. This investigation aims to analyze the impact of pollen addition on the profile of volatile and sensory compounds in Tintilla de Rota warm climate red wines. Volatile compounds were measured by Gas Chromatography–Mass Spectrometry, Odorant Activity Values analysis to find out each compound’s fragrant participation, and sensorial analysis was conducted for a qualified panel of wine-tasters. As a result of the chromatographic analysis, 80 volatile compounds of different chemical families were identified and quantified. Bee pollen increased mainly isoamyl alcohol, esters, and terpenes compounds families in wines. Odorant Activity Values analysis showed an increase in fruity odorant series mainly, followed by floral, for all wines with pollen addition. The sensory analysis showed that low pollen doses (0.1 g/L and 0.25 g/L) increased tasting notes of fruit and floral attributes and fruit and floral odorant series as well, highlighting an increase in red and black fruit notes mainly. On the other hand, high doses deviated the sensory profile towards fleshy stone fruit, and raisin fruit, mostly. In addition, high bee pollen doses produce an increase in the odorant category responsible for the chemical, fatty, and grassy aromas mainly, and high and intermediate dose (1 g/L) an increase in the earthy notes in the aromas. Therefore, low bee pollen doses (0.1 and 0.25 g/L) can improve both the aromatic compound profile, as well as the Odorant Activity Values levels and the sensory profile in Tintilla de Rota red wines.
Genomic Islands in Mycoplasmas
Authors: Citti Christine , Baranowski Eric , Dordet-Frisoni Emilie , Faucher Marion , Nouvel Laurent-Xavier
Published: July 2020

Bacteria of the Mycoplasma genus are characterized by the lack of a cell-wall, the use of UGA as tryptophan codon instead of a universal stop, and their simplified metabolic pathways. Most of these features are due to the small-size and limited-content of their genomes (580–1840 Kbp; 482–2050 CDS). Yet, the Mycoplasma genus encompasses over 200 species living in close contact with a wide range of animal hosts and man. These include pathogens, pathobionts, or commensals that have retained the full capacity to synthesize DNA, RNA, and all proteins required to sustain a parasitic life-style, with most being able to grow under laboratory conditions without host cells. Over the last 10 years, comparative genome analyses of multiple species and strains unveiled some of the dynamics of mycoplasma genomes. This review summarizes our current knowledge of genomic islands (GIs) found in mycoplasmas, with a focus on pathogenicity islands, integrative and conjugative elements (ICEs), and prophages. Here, we discuss how GIs contribute to the dynamics of mycoplasma genomes and how they participate in the evolution of these minimal organisms.
Composition and Structure of Zircon from Hydrothermal Uranium Occurrences of the Litsa Ore Area (Kola Region, Russia)
Authors: Kaulina Tatiana , Lyalina Lyudmila , Kamenetsky Vadim , Il’chenko Vadim , Bocharov Vladimir , Gannibal Maria
Published: July 2020

Zircon grains from various metagranitoids (plagio- and monzo-granites, gneisses, metasomatic rocks, and pegmatoid veins) from the Skal’noe and Dikoe sites of the Litsa uranium ore area (Kola Region, Russia) were studied in order to reconstruct the sequence and timing of events in the area and to observe effects of hydrothermal process related to uranium mineralization on structure and composition of zircon. Individual zircon grains were studied by means of laser ablation inductively coupled plasma mass spectrometry (LA–ICPMS), ion microprobe and Raman spectroscopy. Isotopic LA–ICPMS data for the Skal’noye and Dikoe ore occurrences suggest the following age sequence of events in the area: intrusion of plagiogranites—2829 ± 12 Ma, formation of magmatic protolith of gneisses—2781 ± 17 Ma, metamorphism of plagiogranites—2636 ± 34 Ma; intrusion of monzogranites and pegmatoid veins—2549–2526 Ma, hydrothermal event with uranium input—2276 ± 21 Ma, last metamorphism of plagio- and monzo-granites—1892–1696 Ma. Ore-bearing rocks in the area are pegmatoid veins and quartz–feldspar metasomatites which contain uraninite. During a 2.3 Ga hydrothermal process, newly formed zircon rims grew simultaneously with the precipitation of uraninite in the veins and metasomatites. These rims are characterized by high U and rare earth elements (REE) contents (up to 6560 and 8760 ppm, respectively), dark cathodoluminescence, low Th-U ratios (0.1–0.007) and a flat LREE-enriched pattern, in some cases inherited from minerals, dissolved during a hydrothermal event (magmatic plagioclase and probably monazite). Hydrothermal zircon rims grew with partial dissolution of the magmatic zircon, as evidenced by the rounded and curved shapes of zircon cores. The degree of alteration caused by hydrothermal events depends on the uranium content in the pre-existing zircon. The effects of zircon alteration and newly formed zircon composition reflect the redistribution of uranium in rocks.
Ground Motion Model for Spectral Displacement of Intermediate-Depth Earthquakes Generated by Vrancea Seismic Source
Authors: Olteanu Paul , Vacareanu Radu
Published: July 2020

In support of displacement-based design (DBD), an attenuation model for the prediction of the spectral displacement of intermediate-depth earthquakes generated by Vrancea source is proposed. DBD is an alternative to force-based design, the main benefits being a better and confident description of the structural response and the removal of some of the inconsistencies of force-based code design. The basic input for DBD is the displacement response spectrum (DRS). Vrancea intermediate-depth source is responsible for the seismic hazard for most of the Romanian territory. The source produces, on average, two or three earthquakes with MW >7.0 per century, the prominent characteristics being the large displacement demand and large predominant periods (≈ 1.5 s) for sites located in the Romanian Plain. The model is applicable for sites positioned in front of the South-Eastern Carpathian Arc on type B and C soils. Equations predicting spectral displacement were developed by two-stage regression analysis, using a database containing national analog records of moderate-strong earthquakes and the available digital records, of smaller earthquakes. The model was extended for periods up to 8.0 s using national digital strong motion records and Japanese high-quality digital records of earthquakes triggered by a similar seismo-tectonic environment. The model successfully reproduced observed data, for both type B and C soils and the goodness of fit was tested using methods available in literature.
Should the Glu Be Ten or Twenty? An Update on the Ongoing Debate on Gluten Safety Limits for Patients with Celiac Disease
Authors: Spector Cohen Inna , Day Andrew , Shaoul Ron
Published: July 2020

The only currently accepted and recommended treatment for individuals diagnosed with celiac disease (CD) is a strict life-long gluten-free diet (GFD). While the use of the GFD is well-established, strict adherence to diet is not easy to accomplish. In addition, the administration of a GFD may be compromised by inadvertent exposure to small amounts of gluten. International guidelines define a gluten-free product as one containing less than 20 parts per million (ppm), (20 milligrams of gluten per 1 kg of food) gluten. A number of reports have assessed the safe upper limit for gluten exposure for patients with CD, with general consensus that patients with CD should limit their daily intake to less than 50 mg.
Circulating Tumour DNAs and Non-Coding RNAs as Liquid Biopsies for the Management of Colorectal Cancer Patients
Authors: Lampis Andrea , Ghidini Michele , Ratti Margherita , Mirchev Milko B. , Okuducu Ali Fuat , Valeri Nicola , Hahne Jens Claus
Published: July 2020

Circulating tumour DNAs and non-coding RNAs present in body fluids have been under investigation as tools for cancer diagnosis, disease monitoring, and prognosis for many years. These so-called liquid biopsies offer the opportunity to obtain information about the molecular make-up of a cancer in a minimal invasive way and offer the possibility to implement theranostics for precision oncology. Furthermore, liquid biopsies could overcome the limitations of tissue biopsies in capturing the complexity of tumour heterogeneity within the primary cancer and among different metastatic sites. Liquid biopsies may also be implemented to detect early tumour formation or to monitor cancer relapse of response to therapy with greater sensitivity compared with the currently available protein-based blood biomarkers. Most colorectal cancers are often diagnosed at late stages and have a high mortality rate. Hence, biomolecules as nucleic acids present in liquid biopsies might have prognostic potential and could serve as predictive biomarkers for chemotherapeutic regimens. This review will focus on the role of circulating tumour DNAs and non-coding RNAs as diagnostic, prognostic, and predictive biomarkers in the context of colorectal cancer.
Factors Affecting Dental Service Utilisation in Indonesia: A Population-Based Multilevel Analysis
Authors: Santoso Cornelia Melinda Adi , Bramantoro Taufan , Nguyen Minh Chau , Bagoly Zsuzsa , Nagy Attila
Published: July 2020

This study aimed to examine the prevalence of dental service utilisation in Indonesia and its association with social determinants at individual and community levels. Cross-sectional data from the 2014 Indonesian Family Life Survey (IFLS-5) was analysed. Individual independent variables included age, sex, marital status, educational attainment, economic status, health insurance, dental pain, self-reported mouth ulcers, self-rated health status, unmet healthcare needs and smoking status, while community independent variables included cognitive, structural social capital and residential area. Multilevel logistic regressions were performed to explore the associations between independent variables at different levels and the outcome of dental service utilisation. Of the total sample of 16,860 adults aged 15 years or older in our study, around 86.4% never visited a dentist. Dental service utilisation was associated with older age, female, currently not married, higher education level and economic status, health insurance, dental pain, self-reported mouth ulcers, met healthcare needs, never smoking, living in urban areas and communities with high structural social capital. Both individual and broader social determinants influenced dental service utilisation in Indonesia. These factors should be considered in the formulation of oral health policies and programmes aiming to improve dental service utilisation in the country.
Extremely Low-Frequency Magnetic Fields Exposure Measurement during Lessons in Elementary Schools
Authors: Park JinKyung , Jeong EunHye , Seomun GyeongAe
Published: July 2020

Schools are an important place for children’s exposure to electromagnetic fields, which may cause adverse health effects. To better understand environmental extremely low-frequency magnetic fields (ELF-MFs) exposure among elementary school students, we measured numeric values of ELF-MFs in five classrooms at four schools during digital learning class hours. The measurement of ELF-MFs was taken with an EMDEX II field analyzer. Specifically, we examined the level of exposure to ELF-MFs for each student’s seating position in the classroom. The results showed that ELF-MFs exposure levels were lower than those in the International Commission on Non-Ionizing Radiation Protection guidelines; however, there were significant differences in the level of magnetic field exposure at each school and at each student’s seat. The exposure to ELF-MFs at students’ seat positions was mostly caused by electrical appliances, electronic wiring, and distribution boxes, but the exposure level decreased as the distance increased. Therefore, it is important to design safe and appropriate environments for digital learning in schools, such as proper seating arrangements, to avoid ELF-MFs exposure to students as much as possible. Future studies should measure ELF-MFs levels in other areas and investigate the effects of exposure to ELF-MFs during school hours on children’s health.
Valley Fever: Environmental Risk Factors and Exposure Pathways Deduced from Field Measurements in California
Authors: Lauer Antje , Etyemezian Vicken , Nikolich George , Kloock Carl , Arzate Angel Franco , Sadiq Batcha Fazalath , Kaur Manpreet , Garcia Eduardo , Mander Jasleen , Kayes Passaglia Alyce
Published: July 2020

Coccidioidomycosis, also known as Valley fever, has been reported among military personnel in Coccidioides-endemic areas of the southwestern United States since World War II. In this study, the prevalence of Coccidioides was confirmed in different soil and dust samples collected near three military bases in California using DNA extraction and Polymerase Chain Reaction (PCR) methods. Analyses of physical and chemical parameters revealed no significant differences between Coccidioides-positive and -negative sites. Soil samples collected in the Mojave Desert (near Twentynine Palms MCAGCC) showed the highest percentage of Coccidioides-positive soil and dust samples. Samples from the San Joaquin Valley (near NAS Lemoore) showed the lowest percentage of positive samples and were restricted to remnants of semi-natural areas between agricultural fields. Our results suggest that soil disturbance around all three military bases investigated poses a potential Coccidioides exposure risk for military personnel and the public. We conclude that once lands have been severely disturbed from their original state, they become less suitable for Coccidioides growth. We propose a conceptual framework for understanding exposure where disturbance of soils that exhibit natural or remnants of native vegetation (Creosote and Salt Bush) generate a high risk of exposure to the pathogen, likely during dry periods. In contrast, Coccidioides-positive sites, when undisturbed, will not pose a high risk of exposure.
Women and Physical Activity in Fitness Centres. Analysis of Future Intentions and Their Relationship with Age
Authors: Fernández-Martínez Antonio , Haro-González Mónica , Nuviala Román , Pérez-Ordás Raquel , Nuviala Alberto
Published: July 2020

Physical activity is an important tool for promoting women’s health. Increasing adherence to physical activity is a challenge for governments and private entities. One of the main objectives of the fitness sector is to build customer loyalty. Their behavioural intentions according to gender and age may be a determining factor. The aim of this study was to establish a model that relates the fitness centre’s quality as perceived by female customers, these customers’ future intentions, satisfaction, and age. A total of 745 women participated in this study, with a mean age of 32.97 ± 14.11, divided into three age groups. A confirmatory analysis, a factor invariance analysis, and a multi-group analysis were conducted to verify the validity and reliability of the model. The results revealed that quality is an antecedent of both perceived value and satisfaction in the three age groups. Perceived value is a precursor of satisfaction, except in the group of women over 45 years old. The only antecedent to adaptation to price is quality, except in the older age group. Finally, perceived value was found to be related to adaptation to price. These results will facilitate the development of strategies to promote physical activity among women according to their age.