bluuebunny/crossref_metadata_2025 huggingface dataset:
One-Pot Sol–Gel Synthesis of Highly Insulative Hybrid P(AAm-CO-AAc)-Silica Aerogels with Improved Mechanical and Thermal Properties
Authors: Ransing Akshay A. , Dhavale Rushikesh P. , Parale Vinayak G. , Bangi Uzma K. H. , Choi Haryeong , Lee Wonjun , Kim Jiseung , Wang Qi , Phadtare Varsha D. , Kim Taehee , Jung Wook Ki , Park Hyung-Ho
Published: August 2023

Silica aerogels and their derivatives have outstanding thermal properties with exceptional values in the thermal insulation industry. However, their brittle nature restricts their large-scale commercialization. Thus, enhancing their mechanical strength without affecting their thermal insulating properties is essential. Therefore, for the first time, highly thermally stable poly(acrylamide-co-acrylic acid) partial sodium salt is used as a reinforcing polymer to synthesize hybrid P(AAm-CO-AAc)-silica aerogels via epoxy ring-opening polymerization in the present study. Functional groups in P(AAm-CO-AAc) partial sodium salts, such as CONH2 and COOH, acted as nucleophiles for the epoxy ring-opening reaction with (3-glycidyloxypropyl)trimethoxysilane, which resulted in a seven-fold enhancement in mechanical strength compared to that of pristine silica aerogel while maintaining thermal conductivity at less than 30.6 mW/mK and porosity of more than 93.68%. Moreover, the hybrid P(AAm-CO-AAc)-silica aerogel demonstrated improved thermal stability up to 343 °C, owing to the synergetic effect between the P(AAm-CO-AAc) and the silica aerogel, corresponding to the thermal stability and strong covalent bonding among them. These excellent results illustrate that this new synthetic approach for producing hybrid P(AAm-CO-AAc)-silica aerogels is useful for enhancing the mechanical strength of pristine silica aerogel without impairing its thermal insulating property and shows potential as an industrial heat insulation material.
Effects of Chromium Exposure on the Gene Expression of the Midgut in Silkworms, Bombyx mori
Authors: Rong Wantao , Chen Yazhen , Lu Jieyou , Huang Shuiwang , Xin Lei , Guan Delong , Li Xiaodong
Published: August 2023

Chromium is a severe heavy metal pollutant with significant environmental risks. The effects of Chromium on the digestion of Bombyx mori (silkworms) are of particular importance due to their ecological and economic significance. Herein, RNA sequencing was conducted on nine midgut samples from silkworms exposed to control, 12 g/kg and 24 g/kg Chromium chemical diets. Comparative transcriptomics revealed that under moderate Chromium exposure, there was a significant increase in up-regulated genes (1268 up-regulated to 857 down-regulated), indicating a stimulation response. At higher stress levels, a weakened survival response was observed, with a decrease in up-regulated genes and an increase in down-regulated genes (374 up-regulated to 399 down-regulated). A notable shift in cellular responses under medium chromium exposure was exposed, signifying the activation of crucial metabolic and transport systems and an elevation in cellular stress and toxicity mechanisms. The observation of up-regulated gene expression within xenobiotic metabolism pathways suggests a heightened defense against Chromium-induced oxidative stress, which was primarily through the involvement of antioxidant enzymes. Conversely, high-dose Chromium exposure down-regulates the folate biosynthesis pathway, indicating biological toxicity. Two novel genes responsive to pressure were identified, which could facilitate future stress adaptation understanding. The findings provide insights into the molecular mechanisms underlying silkworms’ digestion response to Chromium exposure and could inform its biological toxicity.
Genetic and Epigenetic Mechanisms of Psoriasis
Authors: Mateu-Arrom Laura , Puig Lluis
Published: August 2023

Psoriasis is a disease involving the innate and adaptative components of the immune system, and it is triggered by environmental factors in genetically susceptible individuals. However, its physiopathology is not fully understood yet. Recent technological advances, especially in genome and epigenome-wide studies, have provided a better understanding of the genetic and epigenetic mechanisms to determine the physiopathology of psoriasis and facilitate the development of new drugs. This review intends to summarize the current evidence on genetic and epigenetic mechanisms of psoriasis.
The 3′ Non-Coding Sequence Negatively Regulates PD-L1 Expression, and Its Regulators Are Systematically Identified in Pan-Cancer
Authors: Chen Zike , Pi Hui , Zheng Wen , Guo Xiaohong , Shi Conglin , Wang Zhiyang , Zhang Jie , Qu Xuanhao , Liu Lehan , Shen Haoliang , Lu Yang , Chen Miaomiao , Zhang Weibing , Sun Rong , Fan Yihui
Published: August 2023

The 3′-untranslated region (3′-UTR) of PD-L1 is significantly longer than the coding sequences (CDSs). However, its role and regulators have been little studied. We deleted whole 3′-UTR region by CRISPR-Cas9. Prognostic analysis was performed using online tools. Immune infiltration analysis was performed using the Timer and Xcell packages. Immunotherapy response prediction and Cox regression was performed using the R software. MicroRNA network analysis was conducted by the Cytoscape software. The level of PD-L1 was significantly and dramatically up-regulated in cells after deleting the 3′-UTR. Additionally, we discovered a panel of 43 RNA-binding proteins (RBPs) whose expression correlates with PD-L1 in the majority of cancer cell lines and tumor tissues. Among these RBPs, PARP14 is widely associated with immune checkpoints, the tumor microenvironment, and immune-infiltrating cells in various cancer types. We also identified 38 microRNAs whose individual expressions are associated with PD-L1 across different cancers. Notably, miR-3139, miR-4761, and miR-15a-5p showed significant associations with PD-L1 in most cancer types. Furthermore, we revealed 21 m6A regulators that strongly correlate with PD-L1. Importantly, by combining the identified RBP and m6A regulators, we established an immune signature consisting of RBMS1, QKI, ZC3HAV1, and RBM38. This signature can be used to predict the responsiveness of cancer patients to immune checkpoint blockade treatment. We demonstrated the critical role of the 3′-UTR in the regulation of PD-L1 and identified a significant number of potential PD-L1 regulators across various types of cancer. The biomarker signature generated from our findings shows promise in predicting patient prognosis. However, further biological investigation is necessary to explore the potential of these PD-L1 regulators.
Relationship between Satisfaction Scores and Racial/Ethnic and Sex Concordance in Primary Care
Authors: Walker Rebekah J. , Dawson Aprill Z. , Thorgerson Abigail , Campbell Jennifer A. , Engel Sara , Kastner Mandy , Egede Leonard E.
Published: August 2023

Racial/ethnic and sex concordance between patients and providers has been suggested as an important consideration in improving satisfaction and increasing health equity. We aimed to guide local efforts by understanding the relationship between satisfaction with care and patient–provider racial/ethnic and sex concordance within our academic medical center’s primary care clinic. Methods: Satisfaction data for encounters from August 2016 to August 2019 were matched to data from the medical record for patient demographics and comorbidities. Data on 33 providers were also obtained, and racial/ethnic and sex concordance between patients and providers was determined for each of the 3672 unique encounters. The primary outcome was top-box scoring on the CGCAHPS overall satisfaction scale (0–8 vs. 9–10). Generalized mixed-effects logistic regression, including provider- and patient-level factors as fixed effects and a random intercept effect for providers, were used to determine whether concordance had an independent relationship with satisfaction. Results: 89.0% of the NHW-concordant pairs and 90.4% of the Minority Race/Ethnicity-concordant pairs indicated satisfaction, while 90.1% of the male-concordant and 85.1% of the female-concordant pairs indicated satisfaction. When fully adjusted, the female-concordant (OR = 0.58, 95% CI 0.35–0.94) and male-discordant (OR = 0.68, 95% CI 0.51–0.91) pairs reported significantly lower top-box satisfaction compared to the male-concordant pairs. Significant differences did not exist in racial/ethnic concordance. Conclusions: In this sample, differences in sex concordance were noted; however, patient- and provider-level factors may be more influential in driving patient satisfaction than race/ethnicity in this health system.
Exercise Training in Non-Hospitalized Patients with Post-COVID-19 Syndrome—A Narrative Review
Authors: Sick Johanna , König Daniel
Published: August 2023

Post COVID-19 Syndrome (PCS) is the persistence of symptoms after an infection with SARS-CoV-2 in both hospitalized and non-hospitalized COVID-19 survivors. Exercise was proposed as a rehabilitation measure for PCS and early studies focused on patients post-hospital discharge. The objective of this review is to summarize the results of trials investigating exercise interventions in non-hospitalized subjects with PCS and propose practical recommendations concerning safe exercise programming. A literature search in the databases MEDLINE and Scopus was conducted until 26 July 2023 and resulted in seven studies that met the criteria. In total, 935 subjects with PCS were investigated. Exercise enhanced aerobic fitness and physical function and relieved symptoms of dyspnea, fatigue and depression. Participants reported lower Post COVID-19 Functional Status scores post-intervention. The exercise programs were well tolerated with no adverse events. To ensure safety, medical examinations should take place in advance and there should be a regular evaluation of the individual responses to the training. Caution is advised when working with patients suffering from post-exertional malaise or diagnosed with myalgic encephalomyelitis/chronic fatigue syndrome. There is a growing need for additional randomized controlled trials to investigate the effectiveness and safety of exercise in individuals with PCS.
Reference Values for Cervical Muscle Strength in Healthy Women Using a Hand-Held Dynamometer and the Association with Age and Anthropometric Variables
Authors: Gorla Camila , Martins Taís de Souza , Florencio Lidiane Lima , Pinheiro-Araújo Carina Ferreira , Fernández-de-las-Peñas César , Martins Jaqueline , Bevilaqua-Grossi Débora
Published: August 2023

Knowledge of reference values for cervical muscle strength is a key tool for clinicians to use as a clinical reference measure and to establish goals during rehabilitation. The objective was to establish reference values for the maximal strength of cervical muscles in healthy women using a handheld dynamometer and verify the association of cervical muscle strength with age and anthropometric measurements. A hundred women were classified into four groups (n = 25) according to age: 18–29 years, 30–39 years, 40–49 years, and 50–60 years. Maximal muscle strength of the cervical spine was measured using a Lafayette® handheld dynamometer for flexion, extension, and bilateral lateral flexion. No differences in cervical muscle strength were observed among the groups (p > 0.05). However, the 18–29-year-old group took less time to reach the peak of force for flexion than the 50–60-year-old group. Moderate correlations were observed between cervical flexor strength and weight, body mass index, and neck circumference, and between cervical extensor strength and weight and body mass index (r = 0.43–0.55; p < 0.05). Reference values for cervical muscle strength in healthy women were established using a handheld dynamometer, and the association between muscle strength and anthropometric data was moderate.
Examination of Cluster Groups of Risk Behaviors and Beliefs Associated with Non-Communicable Diseases with Latent Class Analysis: A Cross-Sectional Study in Rural Bangladesh
Authors: Kobashi Yurie , Haque Syed Emdadul , Amir Isamu , Sakisaka Kayako , Mubassara Sanzida , Tsubokura Masaharu
Published: August 2023

This cross-sectional observational study examined the cluster groups of risk behaviors and beliefs associated with non-communicable diseases (NCDs) and the demographic factors that influence these cluster groups. The questionnaire survey was conducted in Lohagara Upazila in Narail District, Bangladesh and included basic demographics and items associated with NCDs. The inclusion criteria for the participants in this study included those who were aged between 20 and 80 years and both sexes. The survey items were based on risk behavior, belief, and improvement behavior. To identify the several cluster groups based on NCD-related behavior and belief patterns, a log-likelihood latent class analysis was conducted. Then, a multinomial regression analysis was performed to identify the factor associated with each cluster group. Of the 600 participants, 231 (38.5%) had hypertension, 87 (14.5%) had diabetes, and 209 (34.8%) had a body mass index of 25 or more. Finally, risk behaviors and beliefs associated with NCDs were classified into three cluster groups: (1) very high-risk group (n = 58); (2) high-risk group (n = 270); and (3) moderate-risk group (n = 272). The very high-risk group was significantly associated with female gender, older age, fewer years spent in education, and the absence of daily medication compared to the moderate-risk group. Educational interventions in rural Bangladesh should be immediately implemented to improve the risk behaviors and beliefs associated with NCDs.
A Narrative Review on Multi-Domain Instrumental Approaches to Evaluate Neuromotor Function in Rehabilitation
Authors: Scano Alessandro , Guanziroli Eleonora , Brambilla Cristina , Amendola Caterina , Pirovano Ileana , Gasperini Giulio , Molteni Franco , Spinelli Lorenzo , Molinari Tosatti Lorenzo , Rizzo Giovanna , Re Rebecca , Mastropietro Alfonso
Published: August 2023

In clinical scenarios, the use of biomedical sensors, devices and multi-parameter assessments is fundamental to provide a comprehensive portrait of patients’ state, in order to adapt and personalize rehabilitation interventions and support clinical decision-making. However, there is a huge gap between the potential of the multidomain techniques available and the limited practical use that is made in the clinical scenario. This paper reviews the current state-of-the-art and provides insights into future directions of multi-domain instrumental approaches in the clinical assessment of patients involved in neuromotor rehabilitation. We also summarize the main achievements and challenges of using multi-domain approaches in the assessment of rehabilitation for various neurological disorders affecting motor functions. Our results showed that multi-domain approaches combine information and measurements from different tools and biological signals, such as kinematics, electromyography (EMG), electroencephalography (EEG), near-infrared spectroscopy (NIRS), and clinical scales, to provide a comprehensive and objective evaluation of patients’ state and recovery. This multi-domain approach permits the progress of research in clinical and rehabilitative practice and the understanding of the pathophysiological changes occurring during and after rehabilitation. We discuss the potential benefits and limitations of multi-domain approaches for clinical decision-making, personalized therapy, and prognosis. We conclude by highlighting the need for more standardized methods, validation studies, and the integration of multi-domain approaches in clinical practice and research.
Strategies of Screening and Treating Post-Extubation Dysphagia: An Overview of the Situation in Greek-Cypriot ICUs
Authors: Mpouzika Meropi , Iordanou Stelios , Kyranou Maria , Iliopoulou Katerina , Parissopoulos Stelios , Kalafati Maria , Karanikola Maria , Papathanassoglou Elizabeth
Published: August 2023

Post-extubation dysphagia (PED) can lead to serious health problems in critically ill patients. Contrasting its high incidence rate of 12.4% reported in a recent observational study, many ICUs lack routine bedside screening, likely due to limited awareness. This study aimed to establish baseline data on the current approaches and the status of perceived best practices in PED screening and treatment, as well as to assess awareness of PED. A nationwide cross-sectional, online survey was conducted in all fourteen adult ICUs in the Republic of Cyprus in June 2018, with a 100% response rate. Over 85% of ICUs lacked a standard screening protocol for PED. The most commonly reported assessment methods were cough reflex testing and the water swallow test. Treatment approaches included muscle strengthening exercises without swallowing and swallowing exercises. Only 28.6% of ICUs acknowledged PED as a common issue. The study identified significant gaps in awareness and knowledge regarding PED screening and treatment in Greek-Cypriot ICUs. Urgent implementation of comprehensive dysphagia education programs within the units is necessary, and interdisciplinary collaboration among nurses, intensivists, and speech and language therapists is crucial to improve the quality of care provided.
Parental Accompaniment in Operating Rooms Reduces Child Anxiety
Authors: Ejiri Harumi , Imura Hideto , Baba Reizo , Sumi Akiko , Koga Akiko , Kanno Kaoru , Kunimoto Miho , Hayami Kayo , Niimi Teruyuki , Nomoto Shuji , Natsume Nagato
Published: August 2023

Background: We believe that parental presence before the induction of anesthesia for surgery among children with a cleft palate/lip would be effective in mitigating their preoperative anxiety. Objective: We assessed the states of patients with a cleft palate/lip when their parents accompanied them into operating rooms and clarified their and their parents’ cognition using a questionnaire. Methods: Data were collected via nursing observation when patients and their parents entered the operating room. Furthermore, an anonymous questionnaire was administered to patients and parents after the operation regarding their feelings about parental presence in the operating room. Results: In total, nine patients cried when they entered the surgical room. Furthermore, six patients and three parents reported preoperative anxiety. In addition, eight patients agreed that they were satisfied with the presence of their parents before induction. Conclusion: Approximately half of the patients cried. However, the presence of parents before the induction of anesthesia was effective in reducing anxiety among most patients and their parents.
Socioeconomic Inequalities and Factors Associated with the Use of Modern Contraceptive Methods in Women of Childbearing Age in Ecuador, 2018
Authors: Callata-Cardenas Sandra , Peña-Cerna Fátima Milagros del Rosario , Hernández-Vásquez Akram , Azañedo Diego
Published: August 2023

The objective of this study was to determine the socioeconomic inequalities and factors associated with the use of modern contraceptive methods (MCM) in the population of sexually active women of childbearing age in Ecuador. This was an analytical observational study, based on a secondary data analysis of the 2018 National Health and Nutrition Survey (ENSANUT). Information on 19,106 sexually active, married, or cohabiting women between the ages of 15 and 49 were included. Concentration curves (CC) and Erreygers concentration indices (ECI) were calculated, taking into account the use of MCM as the dependent variable and the wealth index as the independent variable. Crude and adjusted prevalence ratios with 95% confidence intervals were calculated using generalized linear models of the Poisson family. We found that 92.8% of the women surveyed used some type of MCM in the last month. A higher educational level presented a significant pro-rich concentration in the use of MCM (EIC: 0.05; p = 0.004). On the other hand, women belonging to the age group of 20 to 29 years (ECI: −0.027; p = 0.027), women with no job (ECI: −0.025; p = 0.004), and non-indigenous women (EIC: −0.031; p < 0.001), presented a pro-poor concentration. Factors significantly associated with MCM use were age, marital status, occupation, parity, ethnicity, area of residence, and living on the coast. In Ecuador, there are socioeconomic inequalities at different levels of population subgroups in women of childbearing age. Measures to promote the use of MCM are required, focusing on groups that present inequality, taking into account the factors associated with their use.
Exploring the Bioprotective Potential of Halophilic Bacteria against Major Postharvest Fungal Pathogens of Citrus Fruit Penicillium digitatum and Penicillium italicum
Authors: Radouane Nabil , Adadi Hasnae , Ezrari Said , Kenfaoui Jihane , Belabess Zineb , Mokrini Fouad , Barka Essaid Ait , Lahlali Rachid
Published: August 2023

Citrus fruits are vulnerable to green mold (caused by Penicillium digitatum) and blue mold (caused by Penicillium italicum) during storage, posing significant challenges to the industry. Therefore, biological control utilizing antagonistic bacteria has emerged as a dependable strategy for managing postharvest diseases. In this study, halophilic bacterial isolates were carefully selected from diverse saline ecosystems, including the Dead Sea, the Agadir Sea, the Rabat Sea, saline soil, and water of the Amassine Oued in Taounate, based on rigorous in vitro and in vivo antagonism bioassays. Out of 21 bacteria from different saline environments, 10 were chosen for further characterization based on the 16S rDNA gene. Notably, the EAM1 isolate demonstrated exceptional inhibitory effects, reaching a 90% inhibition rate against P. digitatum, while the ER2 isolate closely followed with an 89% inhibition rate against P. italicum. Furthermore, in bacterial supernatant experiments, six bacterial isolates effectively curbed the growth of P. digitatum, and three demonstrated efficacy against P. italicum development. In an in vivo trial spanning ten days of incubation, three highly effective isolates against P. digitatum displayed zero severity, and two of these isolates also demonstrated zero severity against P. italicum. Interestingly, a comparison of bacterial filtrates revealed that all isolates exhibited a severity level of over 50% against the pathogen causing green rot (P. digitatum), while the severity was lower than 50% for the supernatants of the two isolates used against P. italicum. In conclusion, this study highlights the promising role of halophilic bacteria, specifically Bacillus amyloliquefaciens EAM1 and B. amyloliquefaciens ER2, in controlling postharvest fruit pathogens. The findings shed light on the potential of utilizing these bioprotective agents to address the challenges posed by green and blue citrus molds, providing valuable insights for the citrus industry.
Effect of Heat Stress on Root Architecture, Photosynthesis, and Antioxidant Profile of Water Spinach (Ipomoea aquatica Forsk) Seedlings
Authors: Wang Xin , Altaf Muhammad Ahsan , Hao Yuanyuan , Wang Zhiwei , Zhu Guopeng
Published: August 2023

Crop productivity around the world is being seriously affected by adverse environmental conditions. High temperature (HT) stress has severely hampered plant growth, yield, and quality. Water spinach is a significant heat-resilient green leafy vegetable that can mitigate prolonged HT stress. However, the morphological, physiological, and biochemical alterations that occur in its response to heat stress remain unknown. In this study, the physiological response to HT stress in water spinach plants with different temperature (25-control, 30, 35, 40, 45 °C) tolerances was investigated. When plants were subjected to HT over a long period of time, their growth was stunted. The results showed that no significant difference was seen between the control (25 °C) and 30 °C for some traits (root shoot fresh weight, root morphological traits, and leaf gas exchanges parameters). Further, HT (35, 40, and 45 °C) stress significantly reduced the growth status, the gas exchange parameters, the pigment content, the photosystem function, and the root architecture system of water spinach. Conversely, HT stress considerably enhanced secondary metabolites in terms of total phenolics, flavonoids, soluble sugars, and anthocyanin content. Furthermore, heat stress remarkably increased the accumulation of reactive oxygen species (ROS) and caused cellular membrane damage. HT stress effectively altered the antioxidant defense system and caused oxidative damage. Generally, HT has an adverse effect on the enzyme activity of water spinach, leading to cell death. However, the current study found that temperatures ≥35 °C had an adverse effect on the growth of water spinach. Further research will be needed to examine the mechanism and the gene expression involved in the cell death that is caused by temperature stress in water spinach plants.
Changes in Soil Nematode and Microbial Community in Cucumber Root-Zone Soil Shaped by Intercropping with Amaranth
Authors: Zhang Xu , Song Mengyuan , Li Jiafan , Liu Xingqun , Gao Lihong , Tian Yongqiang
Published: August 2023

Intercropping systems often contribute to soil health management including inhibiting root-knot nematode disease. The main purpose of this study was to investigate the potential effect of the cucumber-amaranth intercropping system on soil biota, specifically the nematode and microbial communities. Furthermore, the cucumber root-nematode disease was also evaluated. The study found significant effects of cultivation systems (cucumber-amaranth intercropping and cucumber monocropping) and growing seasons (winter-spring (WS) and autumn-winter (AW)) on both soil nematode and microbial community structures in cucumber root-zone soil. Intercropping resulted in a decrease in the relative abundance of Meloidogyne spp., which was consistent with the observed alleviation of root-knot nematode disease. Bacterivorous nematodes were dominant in the intercropping system. The microbial biomass and community-level physiological profiles (CLPP) were generally higher in the intercropping system. Beta diversity analysis showed that the composition of microbial communities varied widely among the treatments and growth seasons. These findings suggest that intercropping with amaranth can regulate soil biota, leading to decreased incidence of root-knot nematodes (RKNs) diseases.
Cultivation Strategies of Kenaf (Hibiscus cannabinus L.) as a Future Approach in Malaysian Agriculture Industry
Authors: Vayabari Diwiyaa A. G. , Ilham Zul , Md Saad Norsharina , Usuldin Siti Rokhiyah Ahmad , Norhisham Danial’ Aizat , Abd Rahim Muhamad Hafiz , Wan-Mohtar Wan Abd Al Qadr Imad
Published: August 2023

In recent years, kenaf has gained significant global attention as a more cost-effective, adaptable, and manageable alternative to other fibre crops. India and China, with nearly 70% of the global kenaf production, have emerged as the leading producers of kenaf plants. While kenaf was traditionally valued for its paper production, it has evolved into a multipurpose crop with diverse industrial applications over the past two decades. Conventional soil-based cultivation methods for kenaf require up to six months for plant maturity. However, in vitro propagation techniques offer a promising alternative that enables faster growth and reduced labour costs. In vitro propagation can be achieved using solid and liquid media, with limited research available on the pure liquid culture method for kenaf. This review aims to introduce and compare the production of kenaf using solid and liquid media, with a specific focus on the emerging country of Malaysia, which seeks to harness the potential of kenaf cultivation for the 15th Sustainable Development Goal, “life on land”, and its contribution to the economy.
Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells
Authors: Yan Manwen , Li Mingyan , Ding Zhuoying , Qiao Fei , Jiang Xuefei
Published: August 2023

Melatonin, a multifunctional signaling molecule, has been shown to play a significant role in response to abiotic stress. Several species have been reported to unveil melatonin’s effect on osmotic stress; however, the signal transduction mechanism of phytohormone-mediated melatonin biosynthesis in plant species remains unclear. In this study, although plants can biosynthesize melatonin, the exogenous application of melatonin to watermelon cells can improve cell growth in response to osmotic stress by regulating the antioxidant machinery of cells. Regarding the melatonin synthesis pathway, ClOMT (ClASMT and ClCOMT) is a multi-gene family, and ClSNAT has two members. Both ClOMTs and ClSNATs harbor the cis-elements in their promoter regions responding to various hormones. Among abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA), ABA treatment observably upregulated the expression of ClOMTs and ClSNATs, and the accumulation of melatonin with ABA treatment reached a level comparable to that with osmotic stress by mannitol treatment. Furthermore, when hormone biosynthesis inhibitors were added to cells before osmotic stress, the expression of ClOMTs and ClSNATs, as well as melatonin accumulation, were significantly suppressed with the ABA biosynthesis inhibitor. This study demonstrated the crucial role of melatonin biosynthesis in response to osmotic stress via plant hormone signal transduction. It showed that ABA signaling plays a dominant role in melatonin synthesis under osmotic stress.
Decoupling of Ecological and Hydrological Drought Conditions in the Limpopo River Basin Inferred from Groundwater Storage and NDVI Anomalies
Authors: Kim Kyung Y. , Scanlon Todd , Bakar Sophia , Lakshmi Venkataraman
Published: August 2023

Droughts are projected to increase in intensity and frequency with the rise of global mean temperatures. However, not all drought indices equally capture the variety of influences that each hydrologic component has on the duration and magnitude of a period of water deficit. While such indices often agree with one another due to precipitation being the major input, heterogeneous responses caused by groundwater recharge, soil moisture memory, and vegetation dynamics may lead to a decoupling of identifiable drought conditions. As a semi-arid basin, the Limpopo River Basin (LRB) is a severely water-stressed region associated with unique climate patterns that regularly affect hydrological extremes. In this study, we find that vegetation indices show no significant long-term trends (S-statistic 9; p-value 0.779), opposing that of the modeled groundwater anomalies (S-statistic -57; p-value 0.05) in the growing season for a period of 18 years (2004–2022). Although the Mann-Kendall time series statistics for NDVI and drought indices are non-significant when basin-averaged, spatial heterogeneity further reveals that such a decoupling trend between vegetation and groundwater anomalies is indeed significant (p-value < 0.05) in colluvial, low-land aquifers to the southeast, while they remain more coupled in the central-west LRB, where more bedrock aquifers dominate. The conclusions of this study highlight the importance of ecological conditions with respect to water availability and suggest that water management must be informed by local vegetation species, especially in the face of depleting groundwater resources.
Antimicrobial Peptides Demonstrate Activity against Resistant Bacterial Pathogens
Authors: Garvey Mary
Published: August 2023

The antimicrobial resistance crisis is an ongoing major threat to public health safety. Low- and middle-income countries are particularly susceptible to higher fatality rates and the economic impact of antimicrobial resistance (AMR). As an increasing number of pathogens emerge with multi- and pan-drug resistance to last-resort antibiotics, there is an urgent need to provide alternative antibacterial options to mitigate disease transmission, morbidity, and mortality. As identified by the World Health Organization (WHO), critically important pathogens such as Klebsiella and Pseudomonas species are becoming resistant to last-resort antibiotics including colistin while being frequently isolated from clinical cases of infection. Antimicrobial peptides are potent amino acid sequences produced by many life forms from prokaryotic, fungal, plant, to animal species. These peptides have many advantages, including their multi-hit mode of action, potency, and rapid onset of action with low levels of resistance being evident. These innate defense mechanisms also have an immune-stimulating action among other activities in vivo, thus making them ideal therapeutic options. Large-scale production and formulation issues (pharmacokinetics, pharmacodynamics), high cost, and protease instability hinder their mass production and limit their clinical application. This review outlines the potential of these peptides to act as therapeutic agents in the treatment of multidrug-resistant infections considering the mode of action, resistance, and formulation aspects. Clinically relevant Gram-positive and Gram-negative pathogens are highlighted according to the WHO priority pathogen list.
Association of Loneliness and Mindfulness in Substance Use Treatment Retention
Authors: Herczyk Johnathan M. , Zullig Keith J. , Davis Stephen M. , Mallow Jennifer , Hobbs Gerald R. , Davidov Danielle M. , Lander Laura R. , Theeke Laurie
Published: August 2023

Background: Elevated mental illness prevalence complicates efforts designed to address the opioid crisis in Appalachia. The recovery community acknowledges that loneliness impacts mood and engagement in care factors; however, the predictive relationship between loneliness and retention in medication-assisted outpatient treatment programs has not been explored. Our objectives were to identify associations between mental health factors and retention in treatment and elucidate treatment retention odds. Data were collected from eighty participants (n = 57 retained, n = 23 not retained) of a mindfulness-based relapse prevention (MBRP) intervention for individuals receiving medication for opioid use disorder (MOUD) in Appalachia. Loneliness, depression, and anxiety did not differ between the retained and not retained, nor did they predict not being retained; however, mindfulness was significantly lower among those not retained in treatment compared to those retained (OR = 0.956, 95% CI (0.912–1.00), and p < 0.05). Preliminary findings provide evidence for mindfulness training integration as part of effective treatment, with aims to further elucidate the effectiveness of mindfulness therapies on symptom reduction in co-occurring mental health disorders, loneliness, and MOUD treatment retention.
Caregiver Quality of Life: Satisfaction and Burnout
Authors: Blom Carolina , Reis Ana , Lencastre Leonor
Published: August 2023

Informal caregivers (ICs) of cancer patients play a crucial role in health care. Several of the challenges they face can affect their quality of life (QoL). This cross-sectional study explored role of burnout and caregiving satisfaction in their relationship to QoL. Portuguese ICs of adult cancer patients (N = 92) answered a sociodemographic and caregiving questionnaire, the WHOQOL-SRPB BREF, assessing physical, psychological, social, environmental, and spiritual QoL domains; the Maslach Burnout Interview, assessing the dimensions of depersonalization, emotional exhaustion, and personal accomplishment; and a Visual Analogic Scale on caregiving satisfaction. We tested correlations and a parallel mediation model for each domain of QoL, considering burnout dimensions as possible mediators between satisfaction and QoL domains. Our results show that satisfaction, burnout dimensions, and almost all QoL domains are correlated. Together, burnout dimensions seem to mediate the relationship between caregiving satisfaction and psychological, environmental, and spiritual QoL. Satisfaction had a significant indirect effect solely through emotional exhaustion on psychological QoL (β = 1.615, 95% BCI [0.590; 2.849]), environmental QoL (β = 0.904, 95% BCI [0.164; 1.876]), and spiritual QoL (β = 0.816, 95% BCI [0.019; 1.792]). It seems essential for mental health professionals to address these dimensions when providing support to an IC.
Using a Cost-Distance Time-Geographic Approach to Identify Red Deer Habitat Use in Banff National Park, Alberta, Canada
Authors: Ho Katherine , Loraamm Rebecca
Published: August 2023

Animal movements are realizations of complex spatiotemporal processes. Central to these processes are the varied environmental contexts in which animals move, which fundamentally impact the movement trajectories of individuals at fine spatial and temporal scales. An emerging perspective in time geography is the direct examination of the influence that varying contexts may have on observed movements. An approach that considers environmental context can yield actionable information for wildlife management, planning, and conservation; for instance, identifying areas of probable occupancy by an animal may improve the efficiency of fieldwork. This research develops the first known practical application of a new cost-distance-based, probabilistic voxel space–time prism (CDBPSTP) in efforts to more realistically characterize the unobserved habitat occupancies of animals occurring between known positions provided by location-aware technologies. The CDBPSTP method is applied to trajectory data collected for a group of red deer (Cervus elaphus) tracked near Banff National Park, Alberta, Canada. As a demonstration of the added value from examining how context influences movement, CDBPSTP habitat occupancy results are compared to the earlier PSTP method in context with empirical and theoretical understandings of red deer habitat preference and space-use behaviors. This comparison reveals that with CDBPSTP, variation present in the mover’s environment is explicitly considered as an influence on the mover’s probable path and occupancies between observations of its location. With the increasing availability of high-resolution geolocational and associated environmental data, this study highlights the potential for CDBPSTP to be leveraged as a broadly applicable tool in animal movement analysis.
Optimization of the Zebrafish Larvae Pentylenetetrazol-Induced Seizure Model for the Study of Caffeine and Topiramate Interactions
Authors: Bartoszek Adrian , Trzpil Alicja , Kozub Anna , Fornal Emilia
Published: August 2023

Epilepsy is a common neurological disorder characterized by seizures that cause neurobiological and behavioral impairment. Caffeine (CAF), which is the most widely consumed stimulant in the world, is reported to influence epileptic seizures and antiepileptic drugs, especially topiramate (TPM). The aim of the study was to optimize the zebrafish larvae pentylenetetrazol-induced seizure model for the study of CAF and TPM interactions, which include the determination of dose space, and the delivery of an analytical method for monitoring CAF, TPM, and CAF metabolite paraxanthine (PAR) in Zebrafish larvae. Methods: The zebrafish larvae, 4 days post-fertilization, were incubated for 18 h with CAF, TPM, or CAF + TPM, with subsequent locomotor activity assessment. Seizures were evoked by adding PTZ solution to obtain a final concentration of 20 mM. Subsequently, the liquid chromatography–mass spectrometry (LC–MS/MS) analytical method was used to simultaneously assess the levels of both CAF and TPM in the larvae. CAF (50 mg/L) and TPM (75 μM) given separately decreased the average larvae locomotor activity compared to the PTZ group but, however, were not able to lower it to the control level. Co-administration of 25 mg/L CAF and 50 μM TPM suppressed the activity to the same level. Adding 25 μM TPM to 50 mg/L CAF decrease the measured CAF level in the larvae. Until proven otherwise, CAF consumption should be regarded as a potential determinant in the modulation of TPM’s efficacy in the management of epileptic seizures. The optimized model will contribute to the standardization of studying CAF and TPM interactions and building the understanding of the molecular bases of the interaction.
Activation of the Renin–Angiotensin–Aldosterone System Is Attenuated in Hypertensive Compared with Normotensive Pregnancy
Authors: Shoemaker Robin , Poglitsch Marko , Huang Hong , Vignes Katherine , Srinivasan Aarthi , Cockerham Cynthia , Schadler Aric , Bauer John A. , O’Brien John M.
Published: August 2023

Hypertension during pregnancy increases the risk of adverse maternal and fetal outcomes, but the mechanisms of pregnancy hypertension are not precisely understood. Elevated plasma renin activity and aldosterone concentrations play an important role in the normal physiologic adaptation to pregnancy. These effectors are reduced in patients with pregnancy hypertension, creating an opportunity to define the features of the renin–angiotensin–aldosterone system (RAAS) that are characteristic of this disorder. In the current study, we used a novel LC-MS/MS-based methodology to develop comprehensive profiles of RAAS peptides and effectors over gestation in a cohort of 74 pregnant women followed prospectively for the development of gestational hypertension and pre-eclampsia (HYP, 27 patients) versus those remaining normotensive (NT, 47 patients). In NT pregnancy, the plasma renin activity surrogate, (PRA-S, calculated from the sum of Angiotensin I + Angiotensin II) and aldosterone concentrations significantly increased from the first to the third trimester, accompanied by a modest increase in the concentrations of angiotensin peptide metabolites. In contrast, in HYP pregnancies, PRA-S and angiotensin peptides were largely unchanged over gestation, and third-trimester aldosterone concentrations were significantly lower compared with those in NT pregnancies. The results indicated that the predominant features of pregnancies that develop HYP are stalled or waning activation of the RAAS in the second half of pregnancy (accompanied by unchanging levels of angiotensin peptides) and the attenuated secretion of aldosterone.
Research Progress on Natural Small-Molecule Compounds for the Prevention and Treatment of Sepsis
Authors: Su Jingqian , Zhou Fen , Wu Shun , Tong Zhiyong
Published: August 2023

Sepsis is a serious disease with high mortality and has been a hot research topic in medical research in recent years. With the continuous reporting of in-depth research on the pathological mechanisms of sepsis, various compounds have been developed to prevent and treat sepsis. Natural small-molecule compounds play vital roles in the prevention and treatment of sepsis; for example, compounds such as resveratrol, emodin, salidroside, ginsenoside, and others can modulate signaling through the NF-κB, STAT3, STAT1, PI3K, and other pathways to relieve the inflammatory response, immunosuppression, and organ failure caused by sepsis. Here, we discuss the functions and mechanisms of natural small-molecule compounds in preventing and treating sepsis. This review will lay the theoretical foundation for discovering new natural small-molecule compounds that can potentially prevent and treat sepsis.
N-Acetylcysteine Amide against Aβ-Induced Alzheimer’s-like Pathology in Rats
Authors: Alkandari Ahmed Fareed , Madhyastha Sampath , Rao Muddanna S.
Published: August 2023

Oxidative stress with a depletion of glutathione is a key factor in the initiation and progression of Alzheimer’s disease (AD). N-Acetylcysteine (NAC), a glutathione precursor, provides neuroprotective effects in AD animal models. Its amide form, N-Acetylcysteine amide (NACA), has an extended bioavailability compared to NAC. This study evaluates the neuroprotective effects of NACA against Aβ1-42 peptide-induced AD-like pathology in rats. Male Wistar rats (2.5 months old) were divided into five groups: Normal Control (NC), Sham (SH), Aβ, Aβ + NACA and NACA + Aβ + NACA (n = 8 in all groups). AD-like pathology was induced by the intracerebroventricular infusion of Aβ1-42 peptide into the lateral ventricle. NACA (75 mg/kg) was administered either as a restorative (i.e., injection of NACA for 7 consecutive days after inducing AD-like pathology (Aβ + N group)), or as prophylactic (for 7 days before and 7 days after inducing the pathology (N + Aβ + N group)). Learning and memory, neurogenesis, expression of AD pathology markers, antioxidant parameters, neuroprotection, astrogliosis and microgliosis were studied in the hippocampus and the prefrontal cortex. All data were analyzed with a one-way ANOVA test followed by Bonferroni’s multiple comparison test. NACA treatment reversed the cognitive deficits and reduced oxidative stress in the hippocampus and prefrontal cortex. Western blot analysis for Tau, Synaptophysin and Aβ, as well as a histopathological evaluation through immunostaining for neurogenesis, the expression of neurofibrillary tangles, β-amyloid peptide, synaptophysin, neuronal morphology and gliosis, showed a neuroprotective effect of NACA. In conclusion, this study demonstrates the neuroprotective effects of NACA against β-amyloid induced AD-like pathology.
A High-Quality Genome Sequence of the Penicillium oxalicum 5-18 Strain Isolated from a Poplar Plantation Provides Insights into Its Lignocellulose Degradation
Authors: Hu Shuang , Zhu Rui , Yu Xing-Ye , Wang Bao-Teng , Ruan Hong-Hua , Jin Feng-Jie
Published: August 2023

Studies on the degradation of plant cell wall polysaccharides by fungal extracellular enzymes have attracted recent attention from researchers. Xylan, abundant in hemicellulose, that play great role in connection between cellulose and lignin, has seen interest in its hydrolytic enzymatic complex. In this study, dozens of fungus species spanning genera were isolated from rotting leaves based on their ability to decompose xylan. Among these isolates, a strain with strong xylanase-producing ability was selected for further investigation by genome sequencing. Based on phylogenetic analysis of ITS (rDNA internal transcribed spacer) and LSU (Large subunit 28S rDNA) regions, the isolate was identified as Penicillium oxalicum. Morphological analysis also supported this finding. Xylanase activity of this isolated P. oxalicum 5-18 strain was recorded to be 30.83 U/mL using the 3,5-dinitro-salicylic acid (DNS) method. Further genome sequencing reveals that sequenced reads were assembled into a 30.78 Mb genome containing 10,074 predicted protein-encoding genes. In total, 439 carbohydrate-active enzymes (CAZymes) encoding genes were predicted, many of which were associated with cellulose, hemicellulose, pectin, chitin and starch degradation. Further analysis and comparison showed that the isolate P. oxalicum 5-18 contains a diverse set of CAZyme genes involved in degradation of plant cell wall components, particularly cellulose and hemicellulose. These findings provide us with valuable genetic information about the plant biomass-degrading enzyme system of P. oxalicum, facilitating a further exploration of the repertoire of industrially relevant lignocellulolytic enzymes of P. oxalicum 5-18.