bluuebunny/crossref_metadata_2025 huggingface dataset:
A Time Series of Snow Density and Snow Water Equivalent Observations Derived From the Integration of GPR and UAV SfM Observations
Authors: McGrath Daniel , Bonnell Randall , Zeller Lucas , Olsen-Mikitowicz Alex , Bump Ella , Webb Ryan , Marshall Hans-Peter
Published: May 2022

Snow depth can be mapped from airborne platforms and measured in situ rapidly, but manual snow density and snow water equivalent (SWE) measurements are time consuming to obtain using traditional survey methods. As a result, the limited number of point observations are likely insufficient to capture the true spatial complexity of snow density and SWE in many settings, highlighting the value of distributed observations. Here, we combine measured two-way travel time from repeat ground-penetrating radar (GPR) surveys along a ∼150 m transect with snow depth estimates from UAV-based Structure from Motion Multi-View Stereo (SfM-MVS) surveys to estimate snow density and SWE. These estimates were successfully calculated on eleven dates between January and May during the NASA SnowEx21 campaign at Cameron Pass, CO. GPR measurements were made with a surface-coupled Sensors and Software PulseEkko Pro 1 GHz system, while UAV flights were completed using a DJI Mavic 2 Pro platform and consisted of two orthogonal flights at ∼60 m elevation above ground level. SfM-MVS derived dense point clouds (DPCs) were georeferenced using eight ground control points and evaluated using three checkpoints, which were distributed across the ∼3.5 ha study plot containing the GPR transect. The DPCs were classified to identify the snow surface and then rasterized to produce snow-on digital surface models (DSMs) at 1 m resolution. Snow depths on each survey date were calculated by differencing these snow-on DSMs from a nearly snow-off DSM collected near the end of the melt season. SfM-derived snow depths were evaluated with independent snow depth measurements from manual probing (mean r2 = 0.67, NMAD = 0.11 m and RMSE = 0.12 m). The GPR-SfM derived snow densities were compared to snow density measurements made in snowpits (r2 = 0.42, NMAD = 39 kg m−3 and RMSE = 68 kg m−3). The integration of SfM and GPR observations provides an accurate, efficient, and a relatively non-destructive approach for measuring snow density and SWE at intermediate spatial scales and over seasonal timescales. Ongoing developments in snow depth retrieval technologies could be leveraged in the future to extend the spatial extent of this method.
Extreme Precipitation Trends and Meteorological Causes Over the Laurentian Great Lakes
Authors: Kunkel Kenneth E. , Yin Xungang , Sun Liqiang , Champion Sarah M. , Stevens Laura E. , Johnson Katharine M.
Published: May 2022

Trends in extreme precipitation and their causes were analyzed for events within the Laurentian Great Lakes for several periods: 1908–2020, 1949–2020, 1980–2019, and 1980–2020. Upward trends in extreme precipitation were found for multiple metrics, including the number of exceedances of return period thresholds for several durations and average recurrence intervals (ARI), the number of extreme basin-average 4-day precipitation totals, and the annual maximum daily station precipitation. The causes of extreme events were classified into 5 meteorological categories: fronts of extratropical cyclones (ETC-FRT), extratropical cyclones but not proximate to the fronts (ETC-NFRT), mesoscale convective systems (MCS), tropical cyclones (TC), and air mass convection (AMC). For daily events exceeding the threshold for 5-yr ARI, ETC-FRTs account for 78% of all events, followed by ETC-NFRTs (12%), MCSs (6%), TCs (2%), and AMC (1%). Upward trends in the number of events by cause were found for all categories except AMC. An examination of basin-wide 4-day extreme events (40 largest events during 1980–2019) found that all events were caused by ETC-FRTs (85%) or ETC-NFRTs (15%).
How Do Bacteria “See” Molecules Inside Themselves?
Authors: Knappenberger Andrew J. , Hiller David A.
Published: May 2022

RNA, like its close cousin DNA, is used to store information in the cell. Unlike DNA, RNA is really good at folding up into interesting shapes, which also makes it good at lots of other important jobs. Some kinds of RNA, called riboswitches, can sense what is going on inside a cell. Each riboswitch fits a specific small molecule. When the riboswitch and small molecule interact, this changes what the cell does. For example, if the small molecule is harmful, the cell might start making a protein that will get rid of it. Recently, scientists discovered some riboswitches that look very similar to each other but recognize very different small molecules. We used a technique called X-ray crystallography to get pictures of these riboswitches, and we saw how changing just one piece of the riboswitch changed which small molecule it recognized. This shows us how RNA can gain new functions as an organism evolves.
HIV-Quasipore: A Suite of HIV-1-Specific Nanopore Basecallers Designed to Enhance Viral Quasispecies Detection
Authors: Link Robert W. , De Souza Diehl R. , Spector Cassandra , Mele Anthony R. , Chung Cheng-Han , Nonnemacher Michael R. , Wigdahl Brian , Dampier Will
Published: May 2022

Accounting for genetic variation is an essential consideration during human immunodeficiency virus type 1 (HIV-1) investigation. Nanopore sequencing preserves proviral integrity by passing long genomic fragments through ionic channels, allowing reads that span the entire genome of different viral quasispecies (vQS). However, this sequencing method has suffered from high error rates, limiting its utility. This was the inspiration behind HIV-Quasipore: an HIV-1-specific Nanopore basecaller suite designed to overcome these error rates through training with gold-standard data. It comprises three deep learning-based R9.4.1 basecallers: fast, high accuracy (HAC), super accuracy (SUP), and two R10.3 deep learning-based basecallers: HAC and SUP. This was accomplished by sequencing the HIV-1 J-Lat 10.6 cell line using Nanopore and high-quality Sanger techniques. Training significantly reduced basecaller error rates across all models (Student’s one-sided t-test; p = 0.0) where median error rates were 0.0189, 0.0018, 0.0008, for R9.4.1 HIV-Quasipore-fast, HAC, SUP, and 0.0007, 0.0011 for R10.3 HIV-Quasipore-HAC, and SUP, respectively. This improved quality reduces the resolution needed to accurately detect a vQS from 22.4 to 2.6% of total positional coverage for R9.4.1 HIV-Quasipore-fast, 6.9 to 0.5% for R9.4.1 HIV-Quasipore-HAC, 4.5 to 0.3% for R9.4.1 HIV-Quasipore-SUP, 8.0 to 0.3% for R10.3 HIV-Quasipore-HAC, and 5.4 to 0.3% for R10.3 HIV-Quasipore-SUP. This was consistently observed across the entire J-Lat 10.6 genome and maintained across longer reads. Reads with greater than 8,000 nucleotides display a median nucleotide identity of 0.9819, 0.9982, and 0.9991, for R9.4.1 HIV-Quasipore-fast, HAC, SUP, and 0.9993, 0.9988 for R10.3 HIV-Quasipore-HAC, and SUP, respectively. To evaluate the robustness of this tool against unseen data, HIV-Quasipore and their corresponding pretrained basecallers were used to sequence the J-Lat 9.2 cell line and a clinical isolate acquired from the Drexel Medicine CARES cohort. When sample reads were compared against their corresponding consensus sequence, all HIV-Quasipore basecallers displayed higher median alignment accuracies than their pretrained counterparts for both the J-Lat 9.2 cell line and clinical isolate. Using Nanopore sequencing can allow investigators to explore topics, such as vQS profile detection, HIV-1 integration site analysis, whole genome amplification, gene coevolution, and CRISPR-induced indel detection, among others. HIV-Quasipore basecallers can be acquired here: https://github.com/DamLabResources/HIV-Quasipore-basecallers.
Body Calibration: Automatic Inter-Task Mapping between Multi-Legged Robots with Different Embodiments in Transfer Reinforcement Learning
Authors: Ikeda Satoru , Kono Hitoshi , Watanabe Kaori , Suzuki Hidekazu
Published: May 2022

Machine learning algorithms are effective in realizing the programming of robots that behave autonomously for various tasks. For example, reinforcement learning (RL) does not require supervision or data sets; the RL agent explores solutions by itself. However, RL requires a long learning time, particularly for actual robot learning situations. Transfer learning (TL) in RL has been proposed to address this limitation. TL realizes fast adaptation and decreases the problem-solving time by utilizing the knowledge of the policy, value function, and Q-function from RL. Taylor proposed TL using inter-task mapping that defines the correspondence between the state and action between the source and target domains. Inter-task mapping is defined based on human intuition and experience; therefore, the effect of TL may not be obtained. The difference in robot shapes for TL is similar to the cognition in the modification of human body composition, and automatic inter-task mapping can be performed by referring to the body representation that is assumed to be stored in the human brain. In this paper, body calibration is proposed, which refers to the physical expression in the human brain. It realizes automatic inter-task mapping by acquiring data modeled on a body diagram that illustrates human body composition and posture. The proposed method is evaluated in a TL situation from a computer simulation of RL to actual robot control with a multi-legged robot.
Nonlinear Covariance Analysis-Based Robust Rendezvous Trajectory Design by Improved Differential Evolution Method
Authors: Jin Kai , Yin Zeyang , Lei Yaolin , Zhang Yuanlong , Zhang Xiaolong
Published: May 2022

This paper presents a robust trajectory design method for approaching and rendezvous with a space target considering multi-source uncertainties. A nonlinear covariance analysis method based on the state transition tensor is presented to formulate the propagation of uncertainties including environment parameter uncertainty, actuator error, sensor noise, navigation error and initial state dispersion of the closed-loop GN&C system. Then, the robust trajectory design problem is defined based on the quantified effect of the uncertainties, and an improved self-adaptive differential evolution algorithm is presented to solve the robust trajectory design problem with uncertainties. Finally, four groups of numerical simulations are carried out to show that the designed robust trajectories can satisfy the final state dispersion constraint under multi-source uncertainties.
A Comprehensive Review of Organochlorine Pesticide Monitoring in Agricultural Soils: The Silent Threat of a Conventional Agricultural Past
Authors: Tzanetou Evangelia N. , Karasali Helen
Published: May 2022

Soil constitutes the central environmental compartment that, primarily due to anthropogenic activities, is the recipient of several contaminants. Among these are organochlorine pesticides (OCPs), which are of major concern, even though they were banned decades ago due to their persistence and the health effects they can elicit. In this review, an overview of monitoring studies regarding OCPs in soils published over the last 30 years along with the development of analytical methods and extraction procedures for their determination in soil are presented. The presented synopsis verifies the soil contamination by OCPs during the last several decades. Soil pollution by OCPs should be an essential aspect of the characterization of whole soil quality, considering that a significant percent of soils on a global scale are in the borderline of suitability for cultivation and pertinent activities. The latter, to an extent, is attributed to the presence of organic contaminants, especially those of persistent chemical natures.
Root Characterization of Myanmar Upland and Lowland Rice in Relation to Agronomic and Physiological Traits under Drought Stress Condition
Authors: Sandar Moe Myint , Ruangsiri Mathurada , Chutteang Cattleya , Arunyanark Anuruck , Toojinda Theerayut , Siangliw Jonaliza L.
Published: May 2022

Lowland and upland rice are the two most important rice ecotypes and are grown under different ecosystems of contrasting soil water conditions. The study aimed to characterize root morphological, anatomical, and architectural traits and shoot physiological traits related to agronomic traits in Myanmar lowland and upland rice under drought conditions. Drought experiments were conducted in the field and in the greenhouse. The reduction in root traits under drought was observed in both lowland and upland rice except for the stele and xylem area. Stele area and xylem area were increased under drought in lowland rice while stele area in upland rice was decreased and was not changed in the xylem area. A positive relationship between agronomic traits and root traits of lowland rice was observed including the total number of nodal roots, root number per tiller, and a negative relationship in lateral root density more than anatomical traits. While upland rice illustrated the relationship between root anatomical traits and agronomic traits. In addition, a negative contribution to biomass by photosynthesis rate, stomatal conductance, and transpiration rate was observed in lowland and upland rice. These findings can help improve drought tolerance in rice by selecting the optimal root trait for each rice ecosystem.
Analysis of Physico-Chemical and Organoleptic Fruit Parameters Relevant for Tomato Quality
Authors: Felföldi Zoltán , Ranga Florica , Roman Ioana A. , Sestras Adriana F. , Vodnar Dan C. , Prohens Jaime , Sestras Radu E.
Published: May 2022

Increased interest in tomato (Solanum lycopersicum L.) production requires breeding to create new cultivars with highly marketable values (e.g., regarding quality, nutrition, and health) and valuable sensorial parameters. The purpose of this research was to compare four tomato commercial hybrids—two newly created and two used as controls in the breeding process, cultivated in a high plastic tunnel, regarding a wide range of physico-chemical properties as well as nutritional and organoleptic components of the fruits, which are relevant for the quality of the tomatoes. The new AS 400 commercial hybrid registered the best results for carotenoids (16.64 mg 100 g−1 FW) and dry matter (6.88%). The highest total ascorbic acid value (28.03 mg 100 g−1 FW) was recorded in the other new hybrid, AS 300, while the highest values of total acidity were recorded on the Precos, used as the control (184.87 mg NaOH 100 g−1 FW). The correlations between the analyzed characteristics and the multivariate analysis provided insight into breeding tomatoes to meet the current fruit quality requirements. Based on the results, hypotheses have been formulated for the creation of new cultivars with anticipatory, prospective character, in order to ensure the future needs of the market and consumers.
The Impact of Noise Anxiety on Behavior and Welfare of Horses from UK and US Owner’s Perspective
Authors: Riva Maria Giorgia , Dai Francesca , Huhtinen Mirja , Minero Michela , Barbieri Sara , Dalla Costa Emanuela
Published: May 2022

Noise anxiety is an over-reaction to loud noises commonly detected among pets and can greatly impact on their welfare and on their management. When exposed to noisy events, horses can show intense escape attempts, which may cause severe accidents for the horse and the rider/handler. The aim of the present study was to investigate, through a web survey, UK and US owners’ perception of noise anxiety severity in their horses, their management strategies and perceived efficacy. The questionnaire was shared via social networking and advertised as “What is your horse afraid of?”. Over a total of 1836 questionnaires filled out; 409 owners reported that their horse has shown unusual behavior during a noise event. A two-step cluster analysis identified two groups: very anxious (VA) and slightly anxious (SA). VA horses were reported to have higher frequency of anxiety behaviors; higher frequency of signs of noise reactivity; and their anxiety did not improve with time. The most used management strategies consisted in providing hay throughout the night, turning in/out their horse or moving it to a paddock. A binomial logistic regression identified that horses that have reported injuries during noise events were more likely to be clustered as VA (OR = 0.24, 95% CI: 0.08–0.76); while providing hay throughout the night was more likely to be very effective management strategy in SA horses (OR = 0.41, 95% CI: 0.16–1.01). Our results confirmed that noise anxiety is a growing behavioral problem that can lead to important welfare concerns for horses. New management strategies, including the use of medicinal products, should be considered to reduce behavioral and physiological signs and help horses to cope with noisy events.
Antimicrobial Efficacy of an Ultraviolet-C Device against Microorganisms Related to Contact Lens Adverse Events
Authors: Dumpati Srikanth , Naroo Shehzad A. , Shah Sunil , Dutta Debarun
Published: May 2022

The purpose of the study was to assess the antimicrobial activity of an ultraviolet-C (UVC) device against microorganisms implicated in contact lens related adverse events. An UVC device with an emitting 4.5 mm diameter Light Emitting Diode (LED; 265 nm; 1.93 mJ/cm2) was used. Pseudomonas aeruginosa, Staphylococcus aureus, Fusarium solani, and Candida albicans agar plate lawns were exposed to the device beams for 15 and 30 s at 8 mm distance. Following the exposure, the diameter of the growth inhibition zone was recorded. Contact lenses made of Delfilicon-A, Senofilicon-A, Comfilicon-A, Balafilicon-A, Samfilicon-A and Omafilicon-A and a commercially available contact storage case was used. They were exposed to bacterial and fungal strains for 18 h at 37 °C and 25 °C respectively. After this, the samples were exposed to UVC for 30 s at 8 mm distance to determine the antimicrobial efficacy. Samples were then gently washed and plated on appropriate agar for enumeration of colonies. The UVC exposure reduced microbial growth by 100% in agar lawns, and significantly (p < 0.05) reduced microbial contamination to contact lenses and cases, ranging between 0.90 to 4.6 log. Very short UVC exposure has high antimicrobial efficacy against most of the predominant causative microorganisms implicated in contact lens related keratitis. UVC could be readily used as a broad-spectrum antimicrobial treatment for lens disinfection.
Ways to Improve Insights into Clindamycin Pharmacology and Pharmacokinetics Tailored to Practice
Authors: Armengol Álvarez Laura , Van de Sijpe Greet , Desmet Stefanie , Metsemakers Willem-Jan , Spriet Isabel , Allegaert Karel , Rozenski Jef
Published: May 2022

Given the increase in bacterial resistance and the decrease in the development of new antibiotics, the appropriate use of old antimicrobials has become even more compulsory. Clindamycin is a lincosamide antibiotic approved for adults and children as a drug of choice for systemic treatment of staphylococcal, streptococcal, and gram-positive anaerobic bacterial infections. Because of its profile and high bioavailability, it is commonly used as part of an oral multimodal alternative for prolonged parenteral antibiotic regimens, e.g., to treat bone and joint or prosthesis-related infections. Clindamycin is also frequently used for (surgical) prophylaxis in the event of beta-lactam allergy. Special populations (pediatrics, pregnant women) have altered cytochrome P450 (CYP)3A4 activity. As clindamycin is metabolized by the CYP3A4/5 enzymes to bioactive N-demethyl and sulfoxide metabolites, knowledge of the potential relevance of the drug’s metabolites and disposition in special populations is of interest. Furthermore, drug–drug interactions derived from CYP3A4 inducers and inhibitors, and the data on the impact of the disease state on the CYP system, are still limited. This narrative review provides a detailed survey of the currently available literature on pharmacology and pharmacokinetics and identifies knowledge gaps (special patient population, drug–drug, and drug–disease interactions) to describe a research strategy for precision medicine.
Taurine Administration Counteracts Aging-Associated Impingement of Skeletal Muscle Regeneration by Reducing Inflammation and Oxidative Stress
Authors: Barbiera Alessandra , Sorrentino Silvia , Fard Damon , Lepore Elisa , Sica Gigliola , Dobrowolny Gabriella , Tamagnone Luca , Scicchitano Bianca Maria
Published: May 2022

Sarcopenia, which occurs during aging, is characterized by the gradual loss of skeletal muscle mass and function, resulting in a functional decline in physical abilities. Several factors contribute to the onset of sarcopenia, including reduced regenerative capacity, chronic low-grade inflammation, mitochondrial dysfunction, and increased oxidative stress, leading to the activation of catabolic pathways. Physical activity and adequate protein intake are considered effective strategies able to reduce the incidence and severity of sarcopenia by exerting beneficial effects in improving the muscular anabolic response during aging. Taurine is a non-essential amino acid that is highly expressed in mammalian tissues and, particularly, in skeletal muscle where it is involved in the regulation of biological processes and where it acts as an antioxidant and anti-inflammatory factor. Here, we evaluated whether taurine administration in old mice counteracts the physiopathological effects of aging in skeletal muscle. We showed that, in injured muscle, taurine enhances the regenerative process by downregulating the inflammatory response and preserving muscle fiber integrity. Moreover, taurine attenuates ROS production in aged muscles by maintaining a proper cellular redox balance, acting as an antioxidant molecule. Although further studies are needed to better elucidate the molecular mechanisms responsible for the beneficial effect of taurine on skeletal muscle homeostasis, these data demonstrate that taurine administration ameliorates the microenvironment allowing an efficient regenerative process and attenuation of the catabolic pathways related to the onset of sarcopenia.
Unveiling the Phytochemical Profile and Biological Potential of Five Artemisia Species
Authors: Trifan Adriana , Zengin Gokhan , Sinan Kouadio Ibrahime , Sieniawska Elwira , Sawicki Rafal , Maciejewska-Turska Magdalena , Skalikca-Woźniak Krystyna , Luca Simon Vlad
Published: May 2022

The Artemisia L. genus comprises over 500 species with important medicinal and economic attributes. Our study aimed at providing a comprehensive metabolite profiling and bioactivity assessment of five Artemisia species collected from northeastern Romania (A. absinthium L., A. annua L., A. austriaca Jacq., A. pontica L. and A. vulgaris L.). Liquid chromatography–tandem high-resolution mass spectrometry (LC-HRMS/MS) analysis of methanol and chloroform extracts obtained from the roots and aerial parts of the plants led to the identification of 15 phenolic acids (mostly hydroxycinnamic acid derivatives), 26 flavonoids (poly-hydroxylated/poly-methoxylated flavone derivatives, present only in the aerial parts), 14 sesquiterpene lactones, 3 coumarins, 1 lignan and 7 fatty acids. Clustered image map (CIM) analysis of the phytochemical profiles revealed that A. annua was similar to A. absinthium and that A. pontica was similar to A. austriaca, whereas A. vulgaris represented a cluster of its own. Correlated with their total phenolic contents, the methanol extracts from both parts of the plants showed the highest antioxidant effects, as assessed by the DPPH and ABTS radical scavenging, CUPRAC, FRAP and total antioxidant capacity methods. Artemisia extracts proved to be promising sources of enzyme inhibitory agents, with the methanol aerial part extracts being the most active samples against acetylcholinesterase and glucosidase. All Artemisia samples displayed good antibacterial effects against Mycobacterium tuberculosis H37Ra, with MIC values of 64–256 mg/L. In conclusion, the investigated Artemisia species proved to be rich sources of bioactives endowed with antioxidant, enzyme inhibitory and anti-mycobacterial properties.
Influence of Climate Change and Land-Use Alteration on Water Resources in Multan, Pakistan
Authors: Abbas Mohsin , Atangana Njock Pierre Guy , Wang Yanning
Published: May 2022

This study presents an evaluation of climate and land-use changes induced impacts on water resources of Multan City, Pakistan. Statistical Down Scaling Model (SDSM) and Geographical Information System (GIS) are used for climate change scenario and spatial analyses. Hydrologic Engineering Center’s Hydraulic Modeling System (HEC-HMS) model is used for rainfall-runoff simulation. The investigated results show significant changes in climatological parameters, i.e., an increase in temperature and decrease in precipitation over the last 40 years, and a significant urban expansion is also observed from 2000 to 2020. The increase in temperature and urbanization has reduced the infiltration rate into the soil and increased the runoff flows. The HEC-HMS results indicate that surface runoff gradually increased over the last two decades. Consequently, the depth of the water table in the shallow aquifer has declined by about 0.3 m/year. Projected climate indices stipulate that groundwater depletion will occur in the future. Arsenic levels have exceeded the permissible limit owing to unplanned urban expansion and open dumping of industrial effluents. The results can help an efficient water resources management in Multan.
A Novel Single-Fed Dual-Band Dual-Circularly Polarized Dielectric Resonator Antenna for 5G Sub-6GHz Applications
Authors: Iqbal Javed , Illahi Usman , Khan Muhammad Abbas , Rauf Abdul , Ali Esraa Mousa , Bari Inam , Ali Haider , Khan Muhammad Amir , Alibakhshikenari Mohammad , Dalarsson Mariana
Published: May 2022

In this research article, a single-fed dual-band circular polarized (CP) dielectric resonator antenna (DRA) for dual-function communication, such as GPS and WLAN, was made. Initially, the proposed design process was initiated by designing a linearly polarized singly fed-DRA. To attain CP fields, the cross-shape conformal metal strip was optimized to excite the fundamental and the high-order mode in the two frequency bands. The metallic strip (parasitic) was utilized on top of the rectangular DRA to improve and widen the impedance and axial ratio (AR) bandwidth. This step led to a 2.73% improvement on the lower band and an impact of 6.5% on the upper band while on the other side a significant improvement was witnessed in the AR bandwidth in both frequency bands. A prototype was designed and fabricated in order to validate its operations. The measurement outcomes of the proposed antennas authenticated wideband impedance bandwidths of 6.4% and 25.26%, and 3-dB axial ratios (AR) of 21.26% and 27.82% respectively. The prototype is a decent candidate for a global positioning system (GPS) and wireless local area network (WLAN).
3D Modelling of Archaeoseismic Damage in the Roman Site of Baelo Claudia (Gibraltar Arc, South Spain)
Authors: Sánchez-Sánchez Yolanda , Elez Javier , Silva Pablo G. , Santos-Delgado Gabriel , Giner-Robles Jorge Luis , Reicherter Klaus
Published: May 2022

This study deals with the morphometric characterization and quantification of earthquake damage in the ancient Roman city of Baelo Claudia in South Spain (Gibraltar Arc) by means of the use of 3D modelling from drone imagery. Baelo Claudia is a world-renowned archaeological site recording recurrent earthquake destruction during the first and third centuries AD. The first earthquake destroyed the lower littoral zone of the city, allowing its reconstruction from the year c. 60–70 CE, but the second earthquake in 365–390 CE led to the complete destruction of the renewed city and its eventual abandonment. This second earthquake imprinted important deformations in the main monumental zone of the city, including the basilica temples, macellum, city walls, aqueducts and funerary monuments, as well as in the main paved zones of the city. This is the case for the Forum, Decumanus and Cardos, which show a variety of folds, pop-up structures, conjugate fractures and impact marks susceptible to be measured in a 3D format. The current study presents detailed (up to 3 mm/pixel) surface models of iconic monuments within the city. The 3D models were obtained by means of serial orthophotos taken with a UAV Mavic Pro 2 (DJI) Drone device equipped with a 20 mpx camera and a 1” CMOS sensor. Each individual image was captured in a geo-referenced jpg format and processed with the Agisoft Metashape Professional software®. Depending on the measured monument, the final images consisted of 250 to 700 photographs clustered by 50,000 to 150,000 tie points. In all studied items (Decumanus, city walls and bath dish), we follow the same workflow of analysis: (1) alignment of photos with support points; (2) building a dense cloud of points; (3) creation of the surface texture; (4) creation of the Digital Elevation Model (DEM); (5) creation of the orthomosaic; and finally, (6) the building of the high-quality 3D tiled surface models. The obtained models allow the geometric quantification of earthquake deformations (displacements, amplitudes, orientation, etc.) in a GIS-based 3D environment suitable to quantify oriented damage of seismic origin. In a complementary way, these 3D models deserve to be considered for their potential role as digital seismoscopes of ancient archaeological sites and/or heritage buildings.
A Comparison of an Adaptive Self-Guarded Honeypot with Conventional Honeypots
Authors: Touch Sereysethy , Colin Jean-Noël
Published: May 2022

To proactively defend computer systems against cyber-attacks, a honeypot system—purposely designed to be prone to attacks—is commonly used to detect attacks, discover new vulnerabilities, exploits or malware before they actually do real damage to real systems. Its usefulness lies in being able to operate without being identified as a trap by adversaries; otherwise, its values are significantly reduced. A honeypot is commonly classified by the degree of interactions that they provide to the attacker: low, medium and high-interaction honeypots. However, these systems have some shortcomings of their own. First, the low and medium-interaction honeypots can be easily detected due to their limited and simulated functions of a system. Second, the usage of real systems in high-interaction honeypots has a high risk of security being compromised due to its unlimited functions. To address these problems, we developed Asgard an adaptive self-guarded honeypot, which leverages reinforcement learning to learn and record attacker’s tools and behaviour while protecting itself from being deeply compromised. In this paper, we compare Asgard and its variant Midgard with two conventional SSH honeypots: Cowrie and a real Linux system. The goal of the paper is (1) to demonstrate the effectiveness of the adaptive honeypot that can learn to compromise between collecting attack data and keeping the honeypot safe, and (2) the benefit of coupling of the environment state and the action in reinforcement learning to define the reward function to effectively learn its objectives. The experimental results show that Asgard could collect higher-quality attacker data compared to Cowrie while evading the detection and could also protect the system for as long as it can through blocking or substituting the malicious programs and some other commands, which is the major problem of the high-interaction honeypot.
Evaluation of the Planetary Boundary Layer Height in China Predicted by the CMA-GFS Global Model
Authors: Long Haichuan , Chen Qiying , Gong Xi , Zhu Keyun
Published: May 2022

The key role of the planetary boundary layer height (PBLH) in pollution, climate, and model forecasting has long been recognized. However, the observed PBLH has rarely been used to evaluate numerical weather prediction models in China. We compared the temporal and spatial characteristics of the bias in the PBLH in China predicted by the CMA-GFS model with vertical high-resolution sounding data and Global Positioning System occultation data from 2019 to 2020. We found that: (1) The PBLH in East China is systematically underestimated by the CMA-GFS model. The bias mainly results from the underestimation of the wind shear in the boundary layer, a smaller sensible heat flux near the surface, and a lower surface temperature. The combined effects of these factors inhibit the boundary layer from developing to a higher height, although the most important contributor is the small sensible heat flux. (2) There is a systematic overestimation of the PBLH over the Tibetan Plateau throughout the year. The bias is mainly a result of the smaller buoyancy, higher wind shear, and larger sensible heat flux forecast by the CMA-GFS model, which drive the boundary layer to develop to a significantly deeper height than the observations. This bias in the CMA-GFS model is mainly caused by the bias in the sensible heat flux and wind shear forecasts. In contrast, the CMA-GFS model underestimates the PBLH in the Tarim Basin. Our preliminary analysis shows that the boundary layer forecasted is unable to develop because the buoyancy effect of the model is too strong. Therefore, the bias of the predicted PBLH by the CMA-GFS model in China is mainly caused by inaccuracies in the sensible heat flux and wind shear forecasts.
Gut Microbiome Suffers from Hematopoietic Stem Cell Transplantation in Childhood and Its Characteristics Are Positively Associated with Intra-Hospital Physical Exercise
Authors: Ugrayová Simona , Švec Peter , Hric Ivan , Šardzíková Sára , Kubáňová Libuša , Penesová Adela , Adamčáková Jaroslava , Pačesová Petra , Horáková Júlia , Kolenová Alexandra , Šoltys Katarína , Kolisek Martin , Bielik Viktor
Published: May 2022

Gut microbiome impairment is a serious side effect of cancer treatment. The aim of this study was to identify the effects of hematopoietic stem cell transplantation (HSCT) treatment on gut microbiota composition in children with acute lymphoblastic leukemia (ALL). Fecal microbiotas were categorized using specific primers targeting the V1–V3 region of 16S rDNA in eligible pediatric ALL patients after HSCT (n = 16) and in healthy controls (Ctrl, n = 13). An intra-hospital exercise program was also organized for child patients during HSCT treatment. Significant differences in gut microbiota composition were observed between ALL HSCT and Ctrl with further negative effects. Plasma C-reactive protein correlated positively with the pathogenic bacteria Enterococcus spp. and negatively with beneficial bacteria Butyriccocus spp. or Akkermansia spp., respectively (rs = 0.511, p = 0.05; rs = −0.541, p = 0.04; rs = −0.738, p = 0.02). Bacterial alpha diversity correlated with the exercise training characteristics. Therefore, specific changes in the microbiota of children were associated with systemic inflammation or the ability to exercise physically during HSCT treatment.
Mapping the Mountains of Giants: Anthropometric Data from the Western Balkans Reveal a Nucleus of Extraordinary Physical Stature in Europe
Authors: Grasgruber Pavel , Mašanović Bojan , Prce Stipan , Popović Stevo , Arifi Fitim , Bjelica Duško , Bokůvka Dominik , Cacek Jan , Davidović Ivan , Gardašević Jovan , Hrazdíra Eduard , Hřebíčková Sylva , Ingrová Pavlína , Potpara Predrag , Stračárová Nikola , Starc Gregor , Mihailović Nataša
Published: May 2022

The inhabitants of the Dinaric Alps (former Yugoslavia and Albania) have long been known as people of impressive body height, but after World War II, there was a critical lack of data related to this phenomenon. This anthropological synthesis includes the measurements of 47,158 individuals (24,642 males and 22,516 females) from the period 2010–2018 and describes detailed regional differences in male stature in the Western Balkans. According to these data, young men from Montenegro (182.9 cm) are currently the tallest 18-year-olds in the world, surpassing their Dutch peers (182.4 cm), and 18-year-old boys from Dalmatia are even taller (183.7 cm) at a regional level. A continuous belt of extraordinary height means (>184 cm) stretches from the Adriatic coast of Dalmatia through Herzegovina to the central part of Montenegro. This article summarizes all the key socio-economic, nutritional, and genetic data, and offers possible explanations for this anthropological phenomenon. Since the remarkable height of the Dinaric populations cannot be connected with any commonly known environmental factor, the most probable hypothesis is genetic and links these physical characteristics with the local founder effect of Y haplogroup I-M170. Furthermore, given that both the level of socio-economic development and dietary protein quality are still sub-optimal, the local upward trend in body height has the potential to continue in the future.
Assessment of MicroRNAs Associated with Tumor Purity by Random Forest Regression
Authors: Nam Dong-Yeon , Rhee Je-Keun
Published: May 2022

Tumor purity refers to the proportion of tumor cells in tumor tissue samples. This value plays an important role in understanding the mechanisms of the tumor microenvironment. Although various attempts have been made to predict tumor purity, attempts to predict tumor purity using miRNAs are still lacking. We predicted tumor purity using miRNA expression data for 16 TCGA tumor types using random forest regression. In addition, we identified miRNAs with high feature-importance scores and examined the extent of the change in predictive performance using informative miRNAs. The predictive performance obtained using only 10 miRNAs with high feature importance was close to the result obtained using all miRNAs. Furthermore, we also found genes targeted by miRNAs and confirmed that these genes were mainly related to immune and cancer pathways. Therefore, we found that the miRNA expression data could predict tumor purity well, and the results suggested the possibility that 10 miRNAs with high feature importance could be used as potential markers to predict tumor purity and to help improve our understanding of the tumor microenvironment.
Receptor Activity Modifying Protein RAMP Sub-Isoforms and Their Functional Differentiation, Which Regulates Functional Diversity of Adrenomedullin
Authors: Shindo Takayuki , Tanaka Megumu , Kamiyoshi Akiko , Ichikawa-Shindo Yuka , Kawate Hisaka , Sakurai Takayuki
Published: May 2022

AM knockout (AM-/-) and RAMP2 knockout (RAMP2-/-) proved lethal for mice due to impaired embryonic vascular development. Although most vascular endothelial cell-specific RAMP2 knockout (E-RAMP2-/-) mice also died during the perinatal period, a few E-RAMP2-/- mice reached adulthood. Adult E-RAMP2-/- mice developed spontaneous organ damage associated with vascular injury. In contrast, adult RAMP3 knockout (RAMP3-/-) mice showed exacerbated postoperative lymphedema with abnormal lymphatic drainage. Thus, RAMP2 is essential for vascular development and homeostasis and RAMP3 is essential for lymphatic vessel function. Cardiac myocyte-specific RAMP2 knockout mice showed early onset of heart failure as well as abnormal mitochondrial morphology and function, whereas RAMP3-/- mice exhibited abnormal cardiac lymphatics and a delayed onset of heart failure. Thus, RAMP2 is essential for maintaining cardiac mitochondrial function, while RAMP3 is essential for cardiac lymphangiogenesis. Transplantation of cancer cells into drug-inducible vascular endothelial cell-specific RAMP2 knockout mice resulted in enhanced metastasis to distant organs, whereas metastasis was suppressed in RAMP3-/- mice. RAMP2 suppresses cancer metastasis by maintaining vascular homeostasis and inhibiting vascular inflammation and pre-metastatic niche formation, while RAMP3 promotes cancer metastasis via malignant transformation of cancer-associated fibroblasts. Focusing on the diverse physiological functions of AM and the functional differentiation of RAMP2 and RAMP3 may lead to the development of novel therapeutic strategies.
Heterologous Expression of Full-Length and Truncated Human ZIP4 Zinc Transporter in Saccharomyces cerevisiae
Authors: Liu Yuting , Bafaro Elizabeth M. , Dempski Robert E.
Published: May 2022

The human (h) transporter hZIP4 is the primary Zn2+ importer in the intestine. hZIP4 is also expressed in a variety of organs such as the pancreas and brain. Dysfunction of hZIP4 can result in the Zn2+ deficiency disease acrodermatitis enteropathica (AE). AE can disrupt digestive and immune system homeostasis. A limited number of hZIP4 expression strategies have hindered increasing knowledge about this essential transmembrane protein. Here, we report the heterologous expression of hZIP4 in Saccharomyces cerevisiae. Both a wild-type and a mutant S. cerevisiae strain, in which the endogenous Zn2+ transporters were deleted, were used to test the expression and localization of an hZIP4–GFP fusion protein. A full-length hZIP4–GFP and a truncated membrane-domain-only (mhZIP4–GFP) protein were observed to be present in the plasma membrane in yeast.
Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury
Authors: Sun Xinlei , Shi Fujie , Wang Weiran , Wu Yunfei , Qu Shuang , Li Jing , Liang Hongwei , Zen Ke
Published: May 2022

Infiltration of polymorphonuclear neutrophils (PMNs) plays a central role in acute lung injury (ALI). The mechanisms governing PMN inflammatory responses, however, remain incompletely understood. Based on our recent study showing a non-metabolic role of pyruvate kinase type M2 (PKM2) in controlling PMN degranulation of secondary and tertiary granules and consequent chemotaxis, here we tested a hypothesis that Pkm2-deficient mice may resist ALI due to impaired PMN inflammatory responses. We found that PMN aerobic glycolysis controlled the degranulation of secondary and tertiary granules induced by fMLP and PMA. Compared to WT PMNs, Pkm2-deficient (Pkm2-/-) PMNs displayed significantly less capacity for fMLP- or PMA-induced degranulation of secondary and tertiary granules, ROS production, and transfilter migration. In line with this, myeloid-specific Pkm2-/- mice exhibited impaired zymosan-induced PMN infiltration in the peritoneal cavity. Employing an LPS-induced ALI mouse model, LPS-treated Pkm2-/- mice displayed significantly less infiltration of inflammatory PMNs in the alveolar space and a strong resistance to LPS-induced ALI. Our results thus reveal that PKM2 is required for PMN inflammatory responses and deletion of PKM2 in PMN leads to an impaired PMN function but protection against LPS-induced ALI.
Hepatitis B Virus Variants with Multiple Insertions and/or Deletions in the X Open Reading Frame 3′ End: Common Members of Viral Quasispecies in Chronic Hepatitis B Patients
Authors: García-García Selene , Caballero-Garralda Andrea , Tabernero David , Cortese Maria Francesca , Gregori Josep , Rodriguez-Algarra Francisco , Quer Josep , Riveiro-Barciela Mar , Homs Maria , Rando-Segura Ariadna , Pacin-Ruiz Beatriz , Vila Marta , Ferrer-Costa Roser , Pumarola Tomas , Buti Maria , Rodriguez-Frias Francisco
Published: May 2022

Deletions in the 3′ end region of the hepatitis B virus (HBV) X open reading frame (HBX) may affect the core promoter (Cp) and have been frequently associated with hepatocellular carcinoma (HCC). The aim of this study was to investigate the presence of variants with deletions and/or insertions (Indels) in this region in the quasispecies of 50 chronic hepatitis B (CHB) patients without HCC. We identified 103 different Indels in 47 (94%) patients, in a median of 3.4% of their reads (IQR, 1.3–8.4%), and 25% (IQR, 13.1–40.7%) of unique sequences identified in each quasispecies (haplotypes). Of those Indels, 101 (98.1%) caused 44 different altered stop codons, the most commonly observed were at positions 128, 129, 135, and 362 (putative position). Moreover, 39 (37.9%) Indels altered the TATA-like box (TA) sequences of Cp; the most commonly observed caused TA2 + TA3 fusion, creating a new putative canonical TATA box. Four (8%) patients developed negative clinical outcomes after a median follow-up of 9.4 (8.7–12) years. In conclusion, we observed variants with Indels in the HBX 3′ end in the vast majority of our CHB patients, some of them encoding alternative versions of HBx with potential functional roles, and/or alterations in the regulation of transcription.
In Vitro Evaluation of Acrylic Adhesives in Lymphatic Fluids-Influence of Glue Type and Procedural Parameters
Authors: Kuetting Daniel , Kupczyk Patrick , Dell Tatjana , Luetkens Julian A. , Meyer Carsten , Attenberger Ulrike I. , Pieper Claus C.
Published: May 2022

To evaluate the embolic properties of different acrylic adhesive/iodized oil mixtures for lymphatic interventions. Polymerization of histoacryl (HA) (Bayer Healthcare) and glubran 2 (GL) (GEM) mixed with iodized oil (ratios 1:0–1:7) were investigated in lymphatic fluids with low and high triglyceride (low TG & high TG) contents. Static polymerization time and dynamic polymerization experiments with different volumes of glucose flush (1, 2 and 5 mL) were performed to simulate thoracic duct embolization. For both glues, static polymerization times were longer when the iodized oil content was increased and when performed in high TG lymphatic fluid. In the dynamic experiments, the prolongation of polymerization due to the oil content and TG levels was less pronounced for both glue types. Increased lymphatic flow rates decreased embolization times for low glue/oil ratios while preventing embolization for high glue/oil ratios. Higher glucose flush volumes increased occlusion times. Polymerization times of acrylic glue in a lymphatic fluid are prolonged by increasing the iodized oil concentration and triglyceride concentration as well as by using larger volumes of glucose flush. Increased lymphatic flow rates decrease embolization times for low glue/oil ratios and may prevent embolization for high glue/oil ratios.
Clinical and Economic Impact of a Multidisciplinary Follow-Up Program in Lymphoma Patients
Authors: Devaux Madeline , Boulin Mathieu , Mounier Morgane , Caillot Denis , Ahwij Nuri , Herbin Adélie , Bastie Jean Noël , Favennec Camille , Robert Philippine , Pistre Pauline , Bost Stephanie , Amiot Pauline , Jacquesson Laurence , Casasnovas Olivier , Rossi Cédric , Gueneau Pauline
Published: May 2022

Objectives: The UMACOACH Lymphoma is a multidisciplinary monitoring program for patients initiating a first highly haematotoxic treatment for Hodgkin or non-Hodgkin lymphoma. Patient follow-up is based on consultation with a pharmacist and planed phone calls by nurses supervised by a clinical haematologist. Our objective was to assess effectiveness and cost of the UMACOACH Lymphoma Program (ULP) and to investigate patient satisfaction and quality of life (QoL). Methods: This French monocentric case-control study included all patients enrolled in the ULP over a one-year period (cases) matched with retrospective patients receiving usual care (controls). Numbers of adverse events (AEs), re-hospitalisations, average relative dose intensity (ARDI), treatment response and survival were compared between the two groups. Among cases, patient satisfaction and QoL using the EORTC-QLQC30 questionnaire before and after treatment were evaluated. Results: Seventy-eight cases were matched to 78 controls. Twenty-six percent grade 3–4 AEs were observed in cases versus 38% in controls (p = 0.001). There were 76 and 88 re-hospitalisations in the case and control groups, respectively (p = 0.217). ARDI > 85% was observed in 92% and 82% of cases and controls, respectively (p = 0.138). No differences were observed in terms of treatment responses and survival. Estimated cost savings were of EUR 81,782 in favour of the case group. An improvement of 5.1 points was observed in the total QoL score before and after treatment in cases. Conclusions: A nurse–pharmacist–haematologist collaboration seems to be promising to reduce grade 3–4 AEs in HL and NHL patients receiving highly haematotoxic chemotherapy regimens. Cost savings from hospitalisation being avoided were also shown.