bluuebunny/crossref_metadata_2025 huggingface dataset:
Gaucher Disease Diagnosis Using Lyso-Gb1 on Dry Blood Spot Samples: Time to Change the Paradigm?
Authors: Dinur Tama , Bauer Peter , Beetz Christian , Kramp Guido , Cozma Claudia , Iurașcu Marius-Ionuț , Becker-Cohen Michal , Istaiti Majdolen , Rolfs Arndt , Zimran Ari , Revel-Vilk Shoshana
Published: January 2022

For years, the gold standard for diagnosing Gaucher disease (GD) has been detecting reduced β-glucocerebrosidase (GCase) activity in peripheral blood cells combined with GBA1 mutation analysis. The use of dried blood spot (DBS) specimens offers many advantages, including easy collection, the need for a small amount of blood, and simpler transportation. However, DBS has limitations for measuring GCase activity. In this paper, we recount our cross-sectional study and publish seven years of experience using DBS samples and levels of the deacylated form of glucocerebroside, glucosylsphingosine (lyso-Gb1), for GD diagnosis. Of 444 screened subjects, 99 (22.3%) were diagnosed with GD at a median (range) age of 21 (1–78) years. Lyso-Gb levels for genetically confirmed GD patients vs. subjects negative to GD diagnosis were 252 (9–1340) ng/mL and 5.4 (1.5–16) ng/mL, respectively. Patients diagnosed with GD1 and mild GBA1 variants had lower median (range) lyso-Gb1, 194 (9–1050), compared to GD1 and severe GBA1 variants, 447 (38–1340) ng/mL, and neuronopathic GD, 325 (116–1270) ng/mL (p = 0.001). Subjects with heterozygous GBA1 variants (carrier) had higher lyso-Gb1 levels, 5.8 (2.5–15.3) ng/mL, compared to wild-type GBA1, 4.9 (1.5–16), ng/mL (p = 0.001). Lyso-Gb1 levels, median (range), were 5 (2.7–10.7) in heterozygous GBA1 carriers with Parkinson’s disease (PD), similar to lyso-Gb1 levels in subjects without PD. We call for a paradigm change for the diagnosis of GD based on lyso-Gb1 measurements and confirmatory GBA1 mutation analyses in DBS. Lyso-Gb1 levels could not be used to differentiate between heterozygous GBA1 carriers and wild type.
Bone Health in Patients with Dyslipidemias: An Underestimated Aspect
Authors: Anagnostis Panagiotis , Florentin Matilda , Livadas Sarantis , Lambrinoudaki Irene , Goulis Dimitrios G.
Published: January 2022

Beyond being aging-related diseases, atherosclerosis and osteoporosis share common pathogenetic pathways implicated in bone and vascular mineralization. However, the contributory role of dyslipidemia in this interplay is less documented. The purpose of this narrative review is to provide epidemiological evidence regarding the prevalence of bone disease (osteoporosis, fracture risk) in patients with dyslipidemias and to discuss potential common pathophysiological mechanisms linking osteoporosis and atherosclerosis. The effect of hypolipidemic therapy on bone metabolism is also discussed. Despite the high data heterogeneity and the variable quality of studies, dyslipidemia, mainly elevated total and low-density lipoprotein cholesterol concentrations, is associated with low bone mass and increased fracture risk. This effect may be mediated directly by the increased oxidative stress and systemic inflammation associated with dyslipidemia, leading to increased osteoclastic activity and reduced bone formation. Moreover, factors such as estrogen, vitamin D and K deficiency, and increased concentrations of parathyroid hormone, homocysteine and lipid oxidation products, can also contribute. Regarding the effect of hypolipidemic medications on bone metabolism, statins may slightly increase BMD and reduce fracture risk, although the evidence is not robust, as it is for omega-3 fatty acids. No evidence exists for the effects of ezetimibe, fibrates, and niacin. In any case, more prospective studies are needed further to elucidate the association between lipids and bone strength.
Macrophage-Mediated Melanoma Reduction after HP-NAP Treatment in a Zebrafish Xenograft Model
Authors: Codolo Gaia , Facchinello Nicola , Papa Nicole , Bertocco Ambra , Coletta Sara , Benna Clara , Dall’Olmo Luigi , Mocellin Simone , Tiso Natascia , de Bernard Marina
Published: January 2022

The Helicobacter pylori Neutrophil Activating Protein (HP-NAP) is endowed with immunomodulatory properties that make it a potential candidate for anticancer therapeutic applications. By activating cytotoxic Th1 responses, HP-NAP inhibits the growth of bladder cancer and enhances the anti-tumor activity of oncolytic viruses in the treatment of metastatic breast cancer and neuroendocrine tumors. The possibility that HP-NAP exerts its anti-tumor effect also by modulating the activity of innate immune cells has not yet been explored. Taking advantage of the zebrafish model, we examined the therapeutic efficacy of HP-NAP against metastatic human melanoma, limiting the observational window to 9 days post-fertilization, well before the maturation of the adaptive immunity. Human melanoma cells were xenotransplanted into zebrafish embryos and tracked in the presence or absence of HP-NAP. The behavior and phenotype of macrophages and the impact of their drug-induced depletion were analyzed exploiting macrophage-expressed transgenes. HP-NAP administration efficiently inhibited tumor growth and metastasis and this was accompanied by strong recruitment of macrophages with a pro-inflammatory profile at the tumor site. The depletion of macrophages almost completely abrogated the ability of HP-NAP to counteract tumor growth. Our findings highlight the pivotal role of activated macrophages in counteracting melanoma growth and support the notion that HP-NAP might become a new biological therapeutic agent for the treatment of metastatic melanomas.
Conformational Flexibility and Local Frustration in the Functional States of the SARS-CoV-2 Spike B.1.1.7 and B.1.351 Variants: Mutation-Induced Allosteric Modulation Mechanism of Functional Dynamics and Protein Stability
Authors: Verkhivker Gennady
Published: January 2022

Structural and functional studies of the SARS-CoV-2 spike proteins have recently determined distinct functional states of the B.1.1.7 and B.1.351 spike variants, providing a molecular framework for understanding the mechanisms that link the effect of mutations with the enhanced virus infectivity and transmissibility. A detailed dynamic and energetic analysis of these variants was undertaken in the present work to quantify the effects of different mutations on functional conformational changes and stability of the SARS-CoV-2 spike protein. We employed the efficient and accurate coarse-grained (CG) simulations of multiple functional states of the D614G mutant, B.1.1.7 and B.1.351 spike variants to characterize conformational dynamics of the SARS-CoV-2 spike proteins and identify dynamic signatures of the functional regions that regulate transitions between the closed and open forms. By combining molecular simulations with full atomistic reconstruction of the trajectories and the ensemble-based mutational frustration analysis, we characterized how the intrinsic flexibility of specific spike regions can control functional conformational changes required for binding with the host-cell receptor. Using the residue-based mutational scanning of protein stability, we determined protein stability hotspots and identified potential energetic drivers favoring the receptor-accessible open spike states for the B.1.1.7 and B.1.351 spike variants. The results suggested that modulation of the energetic frustration at the inter-protomer interfaces can serve as a mechanism for allosteric couplings between mutational sites and the inter-protomer hinges of functional motions. The proposed mechanism of mutation-induced energetic frustration may result in greater adaptability and the emergence of multiple conformational states in the open form. This study suggested that SARS-CoV-2 B.1.1.7 and B.1.351 variants may leverage the intrinsic plasticity of functional regions in the spike protein for mutation-induced modulation of protein dynamics and allosteric regulation to control binding with the host cell receptor.
Maritime Transportation Dynamics in the Azores Region: Analyzing the Period 1998–2019
Authors: Pimentel Pedro , Vulevic Ana , Couto Gualter , Behradfar Arian , Gómez José Manuel Naranjo , Castanho Rui Alexandre
Published: January 2022

The geography over which maritime transportation operates is unique, combining physical, strategic, and commercial imperatives. Physical issues are stable across time, but strategic, especially commercial, considerations continually shift with the ebb and flow of the globalization process. Contextually, the distance that isolates different locations in many cases plays a vital function in these interactions. Distance is the primary element that affects the values of interaction intensities. In fact, the issue is how the intensities were reduced with distance since this reduction is generally not linear. In this regard, this article intends to pursue the issues of the shape and parameters of the distance–decay functions based on the travel time value between islands. In this regard, almost all the Azores Islands were used as a case study. The study results show that the distance–decay functions established the unique dominance of Faial Island, Pico Island, and São Jorge Island, all in the Azores central group; in addition, there was an increase in the number of passengers in those. Moreover, the dominant position is the central islands, and their coexistence with others in the environment makes them more accessible than other islands, showing Terceira Island as a potential maritime passengers’ hub in the Azores. So, with this study, it becomes clear which are the main accessibility issues within the Azores archipelago as well as efficiency should be promoted through the design of policies in maritime passengers’ transportation.
Comment on Costa et al. The Effectiveness of Different Concepts of Bracing in Adolescent Idiopathic Scoliosis (AIS): A Systematic Review and Meta-Analysis. J. Clin. Med. 2021, 10, 2145
Authors: Weiss Hans-Rudolf
Published: January 2022

I read the above-mentioned work with great interest, and I would like to thank the authors for considering two papers from our working group [...]
Improving Performance of the PRYSTINE Traffic Sign Classification by Using a Perturbation-Based Explainability Approach
Authors: Sudars Kaspars , Namatēvs Ivars , Ozols Kaspars
Published: January 2022

Model understanding is critical in many domains, particularly those involved in high-stakes decisions, e.g., medicine, criminal justice, and autonomous driving. Explainable AI (XAI) methods are essential for working with black-box models such as convolutional neural networks. This paper evaluates the traffic sign classifier of the Deep Neural Network (DNN) from the Programmable Systems for Intelligence in Automobiles (PRYSTINE) project for explainability. The results of explanations were further used for the CNN PRYSTINE classifier vague kernels’ compression. Then, the precision of the classifier was evaluated in different pruning scenarios. The proposed classifier performance methodology was realised by creating an original traffic sign and traffic light classification and explanation code. First, the status of the kernels of the network was evaluated for explainability. For this task, the post-hoc, local, meaningful perturbation-based forward explainable method was integrated into the model to evaluate each kernel status of the network. This method enabled distinguishing high- and low-impact kernels in the CNN. Second, the vague kernels of the classifier of the last layer before the fully connected layer were excluded by withdrawing them from the network. Third, the network’s precision was evaluated in different kernel compression levels. It is shown that by using the XAI approach for network kernel compression, the pruning of 5% of kernels leads to a 2% loss in traffic sign and traffic light classification precision. The proposed methodology is crucial where execution time and processing capacity prevail.
Hybrid Assembly Improves Genome Quality and Completeness of Trametes villosa CCMB561 and Reveals a Huge Potential for Lignocellulose Breakdown
Authors: Tomé Luiz Marcelo Ribeiro , da Silva Felipe Ferreira , Fonseca Paula Luize Camargos , Mendes-Pereira Thairine , Azevedo Vasco Ariston de Carvalho , Brenig Bertram , Badotti Fernanda , Góes-Neto Aristóteles
Published: January 2022

Trametes villosa is a wood-decaying fungus with great potential to be used in the bioconversion of agro-industrial residues and to obtain high-value-added products, such as biofuels. Nonetheless, the lack of high-quality genomic data hampers studies investigating genetic mechanisms and metabolic pathways in T. villosa, hindering its application in industry. Herein, applying a hybrid assembly pipeline using short reads (Illumina HiSeq) and long reads (Oxford Nanopore MinION), we obtained a high-quality genome for the T. villosa CCMB561 and investigated its genetic potential for lignocellulose breakdown. The new genome possesses 143 contigs, N50 of 1,009,271 bp, a total length of 46,748,415 bp, 14,540 protein-coding genes, 22 secondary metabolite gene clusters, and 426 genes encoding Carbohydrate-Active enzymes. Our CAZome annotation and comparative genomic analyses of nine Trametes spp. genomes revealed T. villosa CCMB561 as the species with the highest number of genes encoding lignin-modifying enzymes and a wide array of genes encoding proteins for the breakdown of cellulose, hemicellulose, and pectin. These results bring to light the potential of this isolate to be applied in the bioconversion of lignocellulose and will support future studies on the expression, regulation, and evolution of genes, proteins, and metabolic pathways regarding the bioconversion of lignocellulosic residues.
Exploring Verrucosidin Derivatives with Glucose-Uptake-Stimulatory Activity from Penicillium cellarum Using MS/MS-Based Molecular Networking
Authors: Han Junjie , Chen Baosong , Zhang Rui , Zhang Jinjin , Dai Huanqin , Wang Tao , Sun Jingzu , Zhu Guoliang , Li Wei , Li Erwei , Liu Xueting , Yin Wenbing , Liu Hongwei
Published: January 2022

Under the guidance of LC-MS/MS-based molecular networking, seven new verrucosidin derivatives, penicicellarusins A-G (3–9), were isolated together with three known analogues from the fungus Penicillium cellarum. The structures of the new compounds were determined by a combination of NMR, mass and electronic circular dichroism spectral data analysis. The absolute configuration of penicyrone A (10) was corrected based on X-ray diffraction analyses. Bioactivity screening indicated that compounds 1, 2, and 4 showed much stronger promising hypoglycemic activity than the positive drug (rosiglitazone) in the range of 25–100 μM, which represents a potential new class of hypoglycemic agents. Preliminary structure-activity relationship analysis indicates that the formation of epoxy ring on C6-C7 in the structures is important for the glucose uptake-stimulating activity. The gene cluster for the biosynthesis of 1–12 is identified by sequencing the genome of P. cellarum and similarity analysis with the gene cluster of verrucosidins in P. polonicum.
Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment
Authors: Fortin Faubert Maxime , Labrecque Michel , Hijri Mohamed
Published: January 2022

There is a growing interest in plant microbiome’s engineering to optimize desired functions such as improved phytoremediation. This study is aimed at examining the microbial communities inhabiting the roots and rhizospheres of two Salix miyabeana cultivars that had been grown in a short-rotation intensive culture (SRIC) system for six years in a soil contaminated with the discharge from a petrochemical factory. DNA was extracted from roots and rhizospheric soils, and fungal ITS and bacterial and archaeal 16S rDNA regions were amplified and sequenced using Illumina MiSeq technology. Cultivars ‘SX61’ and ‘SX64’ were found to harbor a similar diversity of fungal, bacterial, and archaeal amplicon sequence variants (ASVs). As expected, a greater microbial diversity was found in the rhizosphere biotope than in the roots of both cultivars, except for cultivar ‘SX64’, where a similar fungal diversity was observed in both biotopes. However, we found that microbial community structures were cultivar- and biotope-specific. Although the implication of some identified taxa for plant adaptability and biomass production capacity remains to be explored, this study provides valuable and useful information regarding microbes that could potentially favor the implantation and phytoremediation efficiency of Salix miyabeana in mixed contamination sites in similar climatic environments.
Evaluation of Four Indigenous Non-Saccharomyces Yeasts Isolated from the Shangri-La Wine Region (China) for Their Fermentation Performances and Aroma Compositions in Synthetic Grape Juice Fermentation
Authors: Zhao Yue , Sun Qingyang , Tian Bin , Zhu Shusheng , Du Fei , Mao Ruzhi , Li Su , Liu Lijing , Zhu Yifan
Published: January 2022

This study investigated the fermentation performances and aroma compositions of synthetic grape juice that was fermented by four indigenous non-Saccharomyces yeast isolates that were obtained from the Shangri-La wine region (China): Meyerozyma guilliermondii (AD-58), Saccharomycopsis vini (BZL-28), Saturnispora diversa (BZL-11), and Wickerhamomyces anomalus (DR-110), in comparison to those of Saccharomyces cerevisiae (EC1118). The four indigenous non-Saccharomyces yeasts showed a lower fermentative capacity and a lower conversion rate of sugar to alcohol, but a higher yield of volatile acidity. W. anomalus (DR-110) had a greater ability to produce numerous esters and short-chain fatty acids and the representative flavors of its fermented medium were fruity and fatty. Sac.vini (BZL-28), interestingly, exhibited great capacity in the formation of many monoterpenes, particularly (Z)-β-ocimene, E-β-ocimene, linalool, citral, and geraniol and its fermented medium was characterized by a strong fruity (citrus-like) and floral flavor. M. guilliermondii (AD-58) and Sat. diversa (BZL-11) only mildly affected the aroma profiles of their resultant fermented media, since the concentrations of most of the volatiles that were produced by these two isolates were much lower than their sensory thresholds. The four indigenous non-Saccharomyces yeasts exhibited distinctive fermentation performances and aroma production behaviors. In particularly, W. anomalus (DR-110) and Sac. vini (BZL-28) have shown good potential in enhancing the aromas and complexity of wine.
Kidney Function Change and All-Cause Mortality in Denosumab Users with and without Chronic Kidney Disease
Authors: Wu Ping-Hsun , Lin Ming-Yen , Huang Teng-Hui , Lee Tien-Ching , Lin Sung-Yen , Chen Chung-Hwan , Kuo Mei-Chuan , Chiu Yi-Wen , Chang Jer-Ming , Hwang Shang-Jyh
Published: January 2022

Denosumab is approved for osteoporosis treatment in subjects with and without chronic kidney disease (CKD). Confirmation is required for its safety, treatment adherence, renal function effect, and mortality in patients with CKD. A retrospective cohort study was conducted to compare new users of denosumab in terms of their two-year drug adherence in all participants (overall cohort) and CKD participants (CKD subcohort), which was defined as baseline estimated glomerular filtration rate (eGFR) less than 60 mL/min/1.73 m2. The eGFR was calculated using the 2021 CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation. We defined high adherence (HA) users as receiving three or four doses and low adherence (LA) users as receiving one or two doses. All-cause mortality was analyzed using Kaplan–Meier curves and Cox regression models. In total, there were 1142 subjects in the overall cohort and 500 subjects in the CKD subcohort. HA users had better renal function status at baseline than LD users in the overall cohort. A decline in renal function was only observed among LD users in the overall cohort. In the CKD subcohort, no baseline renal function difference or renal function decline was demonstrated. The all-cause mortality rate of HA users was lower than LA users in both the overall cohort and CKD. A randomized control trial is warranted to target this unique population to confirm our observations.
Lessons from Remarkable FinTech Companies for the Financial Inclusion in Peru
Authors: Velazquez Patricia Vilcanqui , Bobek Vito , Vide Romana Korez , Horvat Tatjana
Published: January 2022

Financial inclusion, defined as the adequate access and usage of formal financial services to improve people’s lives, is a crucial area for the economic development of a country through its various angles. This paper analyzes the impact of selected FinTech companies on financial inclusion in their respective countries to obtain lessons of their business models and country environments that can help Peruvian financial inclusion. The selected FinTechs are M-PESA in Kenya, Nubank in Brazil, GCASH in the Philippines, and Easypaisa in Pakistan, which revolutionized the financial sector in their respective countries. However, a comparative study of their impact on financial inclusion in their respective country has not been conducted yet; therefore, the lessons obtained are helpful for the Peruvian situation due to their practical implications and because they raise possible areas for further and deeper research. The approach of this study considered a qualitative and quantitative method (to find a Pearson correlation between the percentage of the population of Country (A) that are users of FinTech (a) and the six selected demand-side indicators per country retrieved from the Global Findex Database) analysis to understand the results obtained. The results obtained indicate that M-PESA and GCASH, companies specialized in providing basic mobile money transactions such as remittances and withdrawals, did not impact the provision of other financial services such as savings or credit cards. In Easypaisa’s case, this company positively impacts the studied indicators, probably due to its original partnership with a microfinance institution. Regarding Nubank, despite its remarkable growth in the last years, the company does not affect financial inclusion in Brazil yet. Nonetheless, after its recent expansion to provide more financial services, future research could assess the impact of this company on Brazilian financial inclusion.
Quercetin Hybrids—Synthesis, Spectral Characterization and Radical Scavenging Potential
Authors: Kirkova Desislava , Stremski Yordan , Statkova-Abeghe Stela , Docheva Margarita
Published: January 2022

New quercetin-based derivatives are synthesized in an easily accessible one-pot manner. The method is based on the reaction of quercetin with in situ formed electrophilic N-alkoxycarbonylazolium ions. The position of the newly formed C-C bond and structure were spectrally characterized by 1D, 2D 1H, 13C-NMR, IR, and MS analysis. Thus, in all cases, good regioselectivity in the C-8 position for the obtained products was demonstrated. The obtained compounds were evaluated for their DPPH and ABTS free radical scavenging activity and compared to natural compounds—quercetin and rutin.
Lubricity and Rheological Properties of Highly Dispersed Graphite in Clay-Water-Based Drilling Fluids
Authors: Wang Quande , Slaný Michal , Gu Xuefan , Miao Zhipeng , Du Weichao , Zhang Jie , Gang Chen
Published: January 2022

Improving the tribological characteristics of water-based drilling fluids by adding graphene-based lubricants has garnered attention because of the potential for a range of inorganic-material-based additives at high temperature. In this study, we constructed a green and simple adsorption approach to prepare highly dispersed graphite using a cationic surfactant for graphite modification. The findings demonstrated that the prepared graphite was highly dispersed in water and had a low sedimentation rate and small contact angle in distilled water. The concentration dosage of cetyltrimethylammonium chloride (CTAC) on graphite was 0.02 g/g. We evaluated the performance of the modified graphite as a lubricated additive in water-based drilling through a rheological study and viscosity coefficient measurement. The results showed that the viscosity coefficient of drilling fluid with 0.05% modified graphite was reduced by 67% at 180 °C. We proved that the modified graphite can significantly improve the lubrication performance of drilling fluid. Furthermore, we revealed the lubrication mechanism by analyzing the chemical structural and crystalline and morphological features of graphite through a particle size test, zeta potential test, Fourier transform infrared (FTIR) spectroscopy, X-ray powder diffraction (XRD), and scanning electron microscopy (SEM) measurements. The results indicated that the modification of graphite by CTAC only occurs through physical adsorption, without changing the crystal structure. These findings provide a reference for the development of high-performance water-based drilling fluids.
Emerging Glass Industry Patterns in Late Antiquity Balkans and Beyond: New Analytical Findings on Foy 3.2 and Foy 2.1 Glass Types
Authors: Balvanović Roman V. , Šmit Žiga
Published: January 2022

Resolving issues posed by our paper describing the late antiquity glass from Jelica (Serbia), we performed a thorough analysis of similar glass, systematically collected from the literature. The analysis showed that Foy 3.2 type evolved gradually from a composition similar to the Roman antimony-decolorized glass to a composition approaching Foy 2.1, lasting longer (second−seventh century AD) and spreading wider than originally described, including large parts of the Balkans, France interior, Germany, and Britain. The center of its distribution seems to be the Balkans and Italy. During the sixth century, Foy 3.2 glasses in the Balkans showed a significant increase of average MgO concentration compared to the earlier period and Foy 3.2 glasses outside the Balkans, implying different sand quarries and perhaps different trade routes for its imports. Recycling criteria for Foy 3.2 glass has been established. Similarly, 125 high-iron Foy 2.1 glasses are selected from the literature. They cluster within two groups regarding iron concentrations, which we term high iron (HI) and very high iron (VHI) Foy 2.1. In addition, there is a low lime subgroup of the VHI group, termed VHILL. The paper offers two possible explanations for the elevated iron, color branding, and different silica sources. High-iron glasses seem relatively evenly spread across the entire Mediterranean and its interior, representing, on average, around a quarter of the local Foy 2.1 assemblages. The percentages of high-iron samples are almost double in manufactured glass compared to raw glass, suggesting that the addition of iron was happening in the secondary workshops, i.e., for color branding. Among the manufactured glass, the proportions were higher in glassware than in windowpane glass. To capture the changing sand exploitation conditions, we propose the term “generic composition/type” or “(geochemical) class”.
Efficient Sensitized Photoluminescence from Erbium Chloride Silicate via Interparticle Energy Transfer
Authors: Shen Hao , Shang Huabao , Gao Yuhan , Yang Deren , Li Dongsheng
Published: January 2022

In this study, we prepare Erbium compound nanocrystals and Si nanocrystal (Si NC) co-embedded silica film by the sol-gel method. Dual phases of Si and Er chloride silicate (ECS) nanocrystals were coprecipitated within amorphous silica. Effective sensitized emission of Er chloride silicate nanocrystals was realized via interparticle energy transfer between silicon nanocrystal and Er chloride silicate nanocrystals. The influence of density and the distribution of sensitizers and Er compounds on interparticle energy transfer efficiency was discussed. The interparticle energy transfer between the semiconductor and erbium compound nanocrystals offers some important insights into the realization of efficient light emission for silicon-based integrated photonics.
Analysis of the Innovative Channel Strut Concept Manufactured by Roll-Forming
Authors: Kochański Andrzej , Czyżewski Piotr , Cacko Robert , Roznowski Mariusz
Published: January 2022

Due to the wide use of channel strut components, manufacturing is implemented in many industrial plants. Standard technology of profiles is based on welding of two parts of the profile and requires the regalvanizing of the joint zone causes. Thus, the production is challenging to automate on a single line. The main idea of the article is to present a concept of a channel strut, a cold-formed continuous metal component with an open or closed profile section. It would serve as a cantilever support instead of a standard solution. In the article, a unique lock system combination is proposed and analyzed both numerically and experimentally to provide steadiness of the strut without welding or other joining techniques. Two main lock shapes—semicircular and triangle—were proposed with some variations in the cutting plane. Analyses were carried out for three main profile cross-sections with different dimensions, based on the current industrial applications. The semicircular type of the lock was found to be the most stable, giving optimal strength to the strut under assumed loading, comparable to traditional solutions. The commercial FEM software MSC Marc was used for the numerical analysis.
Simulation and Experimental Study on Reverse Helical Milling with the Gradual-Removal Reverse Edge Milling Cutter under Ultrasonic-Assisted Condition
Authors: Ren Kemeng , Wang Guangyue
Published: January 2022

As a new machining method, ultrasonic-assisted bi-direction helical milling has obvious advantages in making holes on carbon fiber-reinforced plastics (CFRP). However, cutting edges of the flat-bottomed milling cutter are easy to wear, which may cause severe defects such as burrs and tears in the outlet of the hole. In order to improve the hole-making quality of CFRP, the gradual-removal reverse edge milling cutter was proposed and designed. The finite method models of reverse helical milling CFRP with the flat-bottomed reverse edge milling cutter and the gradual-removal reverse edge milling cutter under an ultrasonic vibration were established, and the comparative cutting experiments of the two cutters were carried out. By comparing the cutting performance of the two milling cutters under the condition of ultrasonic vibration assistance, the cutting mechanism of improving the hole wall quality by the gradual-removal reverse edge milling cutter was studied. The results showed that when the reverse cumulative cutting depth reached about 60 mm, compared with the flat-bottomed reverse edge milling cutter, the gradual-removal reverse edge milling cutter transferred part of the cutting task of the peripheral edge to the end edge, and the wear of the reverse peripheral edges which directly affects the hole quality was effectively alleviated. This mechanism made the cutting state of the peripheral edge dominated by shear failure, which led to the significant improvement of the quality at the outlet of the hole.
Molecular Details of Actinomycin D-Treated MRSA Revealed via High-Dimensional Data
Authors: Xia Xuewei , Liu Jun , Huang Li , Zhang Xiaoyong , Deng Yunqin , Li Fengming , Liu Zhiyuan , Huang Riming
Published: January 2022

Methicillin-resistant Staphylococcus aureus (MRSA) is highly concerning as a principal infection pathogen. The investigation of higher effective natural anti-MRSA agents from marine Streptomyces parvulus has led to the isolation of actinomycin D, that showed potential anti-MRSA activity with MIC and MBC values of 1 and 8 μg/mL, respectively. Proteomics-metabolomics analysis further demonstrated a total of 261 differential proteins and 144 differential metabolites induced by actinomycin D in MRSA, and the co-mapped correlation network of omics, indicated that actinomycin D induced the metabolism pathway of producing the antibiotic sensitivity in MRSA. Furthermore, the mRNA expression levels of the genes acnA, ebpS, clfA, icd, and gpmA related to the key differential proteins were down-regulated measured by qRT-PCR. Molecular docking predicted that actinomycin D was bound to the targets of the two key differential proteins AcnA and Icd by hydrogen bonds and interacted with multiple amino acid residues of the proteins. Thus, these findings will provide a basic understanding to further investigation of actinomycin D as a potential anti-MRSA agent.
Pembrolizumab-Related Side Effects: Acute Renal Failure and Severe Neurological Toxicity
Authors: Fasano Gabriele , Pabon Ingrid Marcela , Longhitano Yaroslava , Zanza Christian , Carlidi Graziano , Ravera Enrico , Della Selva Andrea
Published: January 2022

Immunotherapy with immune checkpoint inhibitors represents nowadays a marked improvement in cancer treatment. Nevertheless, they can cause severe toxicities that put the patient at high risk, often requiring aggressive treatment. We present the case of a female patient who developed a severe immune-related adverse reaction to Pembrolizumab prescribed for melanoma treatment. Her array of symptoms, which presented a few days after last drug administration, consisted of severe neurological deficit, severe renal failure, polymyositis, and hyperthyroidism. Treatment required the immediate interruption of the trigger drug, infusion of high dose steroids, renal replacement therapy, plasmapheresis, and methimazole, as will be further discussed.
Comparison of ZnO, Al2O3, AlZnO, and Al2O3-Doped ZnO Sensing Membrane Applied in Electrolyte-Insulator-Semiconductor Structures
Authors: Kao Chyuan-Haur , Liu Yi-Wen , Kuo Chih-Chen , Chan Shih-Ming , Wang Deng-Yi , Lin Ya-Hsuan , Lee Ming-Ling , Chen Hsiang
Published: January 2022

In this study, ZnO, AlZnO, Al2O3, and Al2O3-doped ZnO-sensing membranes were fabricated in electrolyte–insulator–semiconductor (EIS) structures. Multiple material analyses indicate that annealing at an appropriate temperature of 500 °C could enhance crystallizations, passivate defects, and facilitate grainizations. Owing to their material properties, both the pH-sensing capability and overall reliability were optimized for these four types of membranes. The results also revealed that higher Al amounts increased the surface roughness values and enhanced larger crystals and grains. Higher Al compositions resulted in higher sensitivity, linearity, and stability in the membrane.
Cellulose Nanofiber-Assisted Dispersion of Halloysite Nanotubes via Silane Coupling Agent-Reinforced Starch–PVA Biodegradable Composite Membrane
Authors: Li Han , Yang Jisheng , Feng Xiaoqiong , Qin Zhiyong
Published: January 2022

HNTs (halloysite nanotubes) are widely used in reinforcing material, often used in material reinforcement and particle loading. However, their easy agglomeration causes them to have great limitations in application. In this work, two kinds of silane coupling agents (KH560 and KH570) were introduced to graft the CNF/HNT (cellulose nanofiber) nanoparticles used to reinforce the starch-polyvinyl alcohol (PVA) composite membranes. The mechanical properties, water resistance properties and thermal performance of the composite membrane were tested. The results showed that the CNF/HNTs nanoparticle system modified by two silane coupling agents enhanced the tensile strength (TS) of the starch–PVA composite membranes by increments of 60.11% and 68.35%, and, in addition, the water resistance of starch–PVA composite membrane improved. The introduction of chemical bonds formed associations and a compact network structure, which increased the thermal stability and the crystallinity of the starch–PVA composite membrane. In the study, we creatively used CNF to disperse HNTs. CNF and HNTs were combined under the action of the silane coupling agent, and then mixed into the starch–PVA membranes matrix to prepare high-performance degradable biological composite membranes.
Effect of Mo Alloying on the Precipitation Behavior of B2 Nano-Particles in Fe-Mn-Al-Ni Shape Memory Alloys
Authors: Yong Liqiu , Zuo Yang , Sun Lixin , Peng Huabei , An Xuguang , Wang Hui , Wen Yuhua
Published: January 2022

In Fe-Mn-Al-Ni shape memory alloys, the stabilization of superelasticity would be affected by the undesired precipitation of B2 nano-particles during natural aging. In order to solve this problem, the effect of Mo alloying on the precipitation behavior of B2 nano-particles during the cooling and natural aging processes was performed by scanning electron microscope, transmission electron microscope and Vickers microhardness test in two Fe-Mn-Al-Ni-Mo shape memory alloys. The results showed that the formation of γ phase was completely suppressed after 15 °C and 80 °C water quenching as well as air cooling. However, B2 nano-particles were still precipitated after the three cooling processes, and their sizes and misfits increased with decreasing the cooling rates. In addition, the Vickers hardness increased after natural aging for 338 days, which indicated that it is not viable to inhibit the precipitation of B2 nano-particles during natural aging by Mo alloying in the Fe-Mn-Al-Ni shape memory alloys.
Least Squares Twin Support Vector Machines to Classify End-Point Phosphorus Content in BOF Steelmaking
Authors: Li Heng , Barui Sandip , Mukherjee Sankha , Chattopadhyay Kinnor
Published: January 2022

End-point phosphorus content in steel in a basic oxygen furnace (BOF) acts as an indicator of the quality of manufactured steel. An undesirable amount of phosphorus is removed from the steel by the process of dephosphorization. The degree of phosphorus removal is captured numerically by the ‘partition ratio’, given by the ratio of %wt phosphorus in slag and %wt phosphorus in steel. Due to the presence of multitudes of process variables, often, it is challenging to predict the partition ratio based on operating conditions. Herein, a robust data-driven classification technique of least squares twin support vector machines (LSTSVM) is applied to classify the ‘partition ratio’ to two categories (‘High’ and ‘Low’) steels indicating a greater or lesser degree of phosphorus removal, respectively. LSTSVM is a simpler, more robust, and faster alternative to the twin support vector machines (TWSVM) with respect to non-parallel hyperplanes-based binary classifications. The relationship between the ‘partition ratio’ and the chemical composition of slag and tapping temperatures is studied based on approximately 16,000 heats from two BOF plants. In our case, a relatively higher model accuracy is achieved, and LSTSVM performed 1.5–167 times faster than other applied algorithms.
RGB-D Visual SLAM Based on Yolov4-Tiny in Indoor Dynamic Environment
Authors: Chang Zhanyuan , Wu Honglin , Sun Yunlong , Li Chuanjiang
Published: January 2022

For a SLAM system operating in a dynamic indoor environment, its position estimation accuracy and visual odometer stability could be reduced because the system can be easily affected by moving obstacles. In this paper, a visual SLAM algorithm based on the Yolov4-Tiny network is proposed. Meanwhile, a dynamic feature point elimination strategy based on the traditional ORBSLAM is proposed. Besides this, to obtain semantic information, object detection is carried out when the feature points of the image are extracted. In addition, the epipolar geometry algorithm and the LK optical flow method are employed to detect dynamic objects. The dynamic feature points are removed in the tracking thread, and only the static feature points are used to estimate the position of the camera. The proposed method is evaluated on the TUM dataset. The experimental results show that, compared with ORB-SLAM2, our algorithm improves the camera position estimation accuracy by 93.35% in a highly dynamic environment. Additionally, the average time needed by our algorithm to process an image frame in the tracking thread is 21.49 ms, achieving real-time performance.
Design and Construction of Cu(OH)2/Ni3S2 Composite Electrode on Cu Foam by Two-Step Electrodeposition
Authors: Lv Sa , Shang Wenshi , Wang Huan , Chu Xuefeng , Chi Yaodan , Wang Chao , Yang Jia , Geng Peiyu , Yang Xiaotian
Published: January 2022

A Cu(OH)2/Ni3S2 composite has been designed and in situ constructed on Cu foam substrate by facile two-step electrodeposition. Cu(OH)2 is achieved on Cu foam by galvanostatic electrodeposition, and the subsequent coating of Ni3S2 is realized by cyclic voltammetric (CV) electrodeposition. The introduction of Cu(OH)2 provides skeleton support and a large specific surface area for the Ni3S2 electrodeposition. Benefiting from the selection of different components and preparation technology, the Cu(OH)2/Ni3S2 composite exhibits enhanced electrochemical properties with a high specific capacitance of 4.85 F cm−2 at 2 mA cm−2 and long-term cyclic stability at 80.84% (4000 cycles).
Rod Mill Product Control and Its Relation to Energy Consumption: A Case Study
Authors: Anticoi Hernan , Guasch Eduard , Pérez-Álvarez Rubén , de Luis-Ruiz Julio Manuel , Oliva Josep , Hoffman Sampaio Carlos
Published: January 2022

Energy consumption and pollution are current strategic issues that need to be addressed in the mining industry. Both have an economic and environmental impact on production, so their optimization, control, and mitigation are, at the very least, mandatory. Although rod milling has fallen into disuse in recent decades, some companies still use it in their processing plants. This is due to the ability of rod milling to reduce particle size while avoiding overgrinding. In this study, a material that is particularly difficult to characterize was used to study how to control rod-milling particle size distribution product: potash ore, which is deliquescent and soluble under certain conditions. A laboratory-scale tumbling rod mill was designed for this study, and six operative parameters were tested and analyzed in order to detect the main influences on the mill product, attending to material requirements for further processes such as recirculation load or froth flotation for beneficiation. Although the rotational speed of the mill is the parameter that shows the greatest reduction in energy consumption, reaching almost 40% improvement in specific energy applied to the particles, it is not possible to control particle size reduction ratio. However, when a low percentage of grinding media is used, it reduces around 25% of the energy used and, in turn, reduces the amount of overgrinding (40% reduction in the F300 control parameter, for example), which is a strategic objective of this study. In addition, by controlling other process parameters, such as slurry density or lifter geometry, energy consumption and its subsequent saving and pollution can be controlled, depending on process plant requirements.
Effect of Grinding Media Size on Ferronickel Slag Ball Milling Efficiency and Energy Requirements Using Kinetics and Attainable Region Approaches
Authors: Petrakis Evangelos , Komnitsas Konstantinos
Published: January 2022

The aim of this study is to evaluate the effect that the size of grinding media exerts on ferronickel slag milling efficiency and energy savings. A series of tests were performed in a laboratory ball mill using (i) three loads of single size media, i.e., 40, 25.4, and 12.7 mm and (ii) a mixed load of balls with varying sizes. In order to simulate the industrial ball milling operation, the feed to the mill consisted of slag with natural size distribution less than 850 μm. Grinding kinetic modeling and the attainable region (AR) approach were used as tools to evaluate the data obtained during the ball milling of slag. Particular importance was given to the determination of the specific surface area of the grinding products, the identification of the grinding limit, and the maximum specific surface area which could be achieved when different grinding media sizes were used. The results showed that, in general, the breakage rates of particles obey non-first-order kinetics and coarse particles are ground more efficiently than fines. The AR approach proved that there is an optimal grinding time (or specific energy input) dependent on the ball size used for which the volume fraction of the desired size class is maximized. The use of either 25.4 mm balls or a mixed load of balls with varying sizes results in 31 and 24% decrease in energy requirements, compared to the use of balls with small size (12.7 mm).
Orthogonal Numerical Analysis of Deformation and Failure Characteristics of Deep Roadway in Coal Mines: A Case Study
Authors: Feng Xiaojun , Ding Zeng , Hu Qinjing , Zhao Xue , Ali Muhammad , Banquando Jefferson T.
Published: January 2022

With the development of deep, underground coal mines in China, the failure mechanism of the rocks surrounding roadways is becoming increasingly complicated and the deformational control is also significantly difficult. In this study, based on the temporal and spatial deformational distribution of the deep roadway area in the 2233 working face of Fuxin Hengda Coal Mine, factors affecting the deformation and failure mechanism of deep-buried roadways, such as cohesion (c), tensile strength (σt), internal friction angle (φ), vertical ground stress (p), and the horizontal-to-vertical stress ratio (λ), were analyzed using orthogonal numerical experiments. The stress and electromagnetic radiation monitoring data were used to locate areas of highly concentrated deformation in the roadway and surrounding rocks. The results show that the order of the degree of influence of the surrounding rock and geometric parameters on the deformation of the deep-buried roadway is φ > p > σt > λ > c. The vertical stress of the roof and the horizontal stress of the two sides are negatively correlated with the tensile strength and horizontal-to-vertical stress ratio, respectively, and mainly shear failure occurs in the area. The higher the level of the surrounding rock, the more serious is the deterioration and deformation. The electromagnetic radiation reflects the distribution range of the high-stress concentration area and strength deterioration area. The test results accurately describe the deformation–deterioration-failure laws of rock surrounding deep-buried roadways influenced by different factors. The results are of great significance for analyzing the deformation and failure characteristics of rocks surrounding roadways, preventing rockburst, and supporting the parameter optimization of roadways.