bluuebunny/crossref_metadata_2025 huggingface dataset:
Reasons for Initiation and Regular Use of Heated Tobacco Products among Current and Former Smokers in South Korea: Findings from the 2020 ITC Korea Survey
Authors: Seo Hong Gwan , Xu Steve S. , Li Grace , Gravely Shannon , Quah Anne C. K. , Lee Sungkyu , Lim Sujin , Cho Sung-il , Kim Yeol , Lee Eon Sook , Choi Yoon-Jung , Miller Connor R. , Goniewicz Maciej L. , O’Connor Richard J. , Fong Geoffrey T.
Published: March 2023

South Korea is the world’s second-largest heated tobacco product (HTP) market after Japan. HTP sales in South Korea have increased rapidly since May 2017, accounting for 10.6% of the total tobacco market in 2020. Despite this, little is known as to why HTP consumers who were current and former smokers started using HTPs and used them regularly. We analyzed cross-sectional data for 1815 adults (aged 19+) who participated in the 2020 International Tobacco Control (ITC) Korea Survey, of whom 1650 were HTP-cigarette consumers (those who reported smoking cigarettes and using HTPs ≥ weekly) and 165 were exclusive HTP consumers (using HTPs ≥ weekly) who were former or occasional smokers (smoking cigarette < weekly). Respondents were asked to report the reason(s) they used HTPs, with 25 possible reasons for HTP-cigarette consumers and 22 for exclusive HTP consumers. The most common reasons for initiating HTP use among all HTP consumers were out of curiosity (58.9%), family and friends use HTPs (45.5%), and they like the HTP technology (35.9%). The most common reasons for regularly using HTPs among all HTP consumers were that they were less smelly than cigarettes (71.3%), HTPs are less harmful to own health than cigarettes (48.6%), and stress reduction (47.4%). Overall, 35.4% of HTP-cigarette consumers reported using HTPs to quit smoking, 14.7% to reduce smoking but not to quit, and 49.7% for other reasons besides quitting or reducing smoking. In conclusion, several common reasons for initiating and regularly using HTPs were endorsed by all HTP consumers who were smoking, had quit smoking completely, or occasionally smoked. Notably, only about one-third of HTP-cigarette consumers said they were using HTPs to quit smoking, suggesting that most had no intention of using HTPs as an aid to quit smoking in South Korea.
Development and Evaluation of a Workplace Bullying Cognitive Rehearsal-Based Nursing Simulation Education Program: A Mixed-Methods Study
Authors: Park Mijeong , Choi Jeong Sil
Published: March 2023

Bullying makes learning difficult for nursing students, and using real-life scenarios during training can improve the understanding of workplace bullying. Thus, to mitigate bullying experienced by nurses, this study developed and evaluated a cognitive rehearsal education program that constituted training nursing students through role-play simulations. A mixed-method research design was used to evaluate 39 nursing students from two universities. A quasi-experimental research design was applied to assess symptoms, knowledge, and perceptions of bullying, and a focus group interview was conducted with six participants. Quantitative analyses revealed that the program improved participants’ knowledge and perceptions but not their symptoms. The focus group interview revealed that the program improved participants’ coping skills and desire for education. This program could be effective in raising awareness of workplace bullying and increasing relevant coping skills. It can be further developed as part of an overall strategy to manage workplace bullying and its consequences in hospital settings.
Transformative Social and Emotional Learning (T-SEL): The Experiences of Teenagers Participating in Volunteer Club Activities in the Community
Authors: Cojocaru Stefan
Published: March 2023

Beginning with social inequities in terms of access to quality, inclusive education for children from disadvantaged backgrounds, especially rural teenagers who leave school early, the Holtis Association, with the support of the UNICEF Representative in Romania, developed a number of interventions intended to facilitate the transition from lower to higher secondary education of students from vulnerable and disadvantaged groups. One of the interventions was the establishment of teenagers’ clubs for volunteer activities, leadership development, and participation in the community to encourage social and emotional learning. (1) Background: This study aims to investigate the extent to which participation in the Holtis club projects contributed to the development of transformative social and emotional learning (T-SEL), as observed from the perspective of the Collaborative for Academic, Social and Emotional Learning (CASEL) competencies among adolescents. (2) Methods: The study was qualitative and used focus groups for data collection. Out of the 65 active clubs, 18 were selected, and their representatives participated in the focus groups. (3) Results: Participating in the club activities, which were organized in the school, with the aim of organizing activities outside the school space stimulated and developed T-SEL competencies among adolescents. (4) Conclusions: The data, which were collected through the voices of teenagers, underlined the personal transformation from the perspective of the CASEL model competencies of SEL, and the study privileged their perspectives.
Clustering of Environmental Parameters and the Risk of Acute Ischaemic Stroke
Authors: Koo Geraldine P. Y. , Zheng Huili , Aik Joel C. L. , Tan Benjamin Y. Q. , Sharma Vijay K. , Sia Ching Hui , Ong Marcus E. H. , Ho Andrew F. W.
Published: March 2023

Acute ischaemic stroke (AIS) risk on days with similar environmental profiles remains unknown. We investigated the association between clusters of days with similar environmental parameters and AIS incidence in Singapore. We grouped calendar days from 2010 to 2015 with similar rainfall, temperature, wind speed, and Pollutant Standards Index (PSI) using k-means clustering. Three distinct clusters were formed ‘Cluster 1’ containing high wind speed, ‘Cluster 2’ having high rainfall, and ‘Cluster 3’ having high temperatures and PSI. We aggregated the number of AIS episodes over the same period with the clusters and analysed their association using a conditional Poisson regression in a time-stratified case-crossover design. Comparing the three clusters, Cluster 3 had the highest AIS occurrence (IRR 1.09; 95% confidence interval (CI) 1.05–1.13), with no significant difference between Clusters 1 and 2. Subgroup analyses in Cluster 3 showed that AIS risk was amplified in the elderly (≥65 years old), non-smokers, and those without a history of ischaemic heart disease/atrial fibrillation/vascular heart disease/peripheral vascular disease. In conclusion, we found that AIS incidence may be higher on days with higher temperatures and PSI. These findings have important public health implications for AIS prevention and health services delivery during at-risk days, such as during the seasonal transboundary haze.
A Qualitative Study on the Use of the Hospital Safety Index and the Formulation of Recommendations for Future Adaptations
Authors: Lamine Hamdi , Lamberti-Castronuovo Alessandro , Singh Prinka , Chebili Naoufel , Zedini Chekib , Achour Nebil , Valente Martina , Ragazzoni Luca
Published: March 2023

The Hospital Safety Index is a tool developed by the World Health Organization and the Pan American Health Organization in 2008 and updated in 2015. Although it is the most widely used instrument of its kind to assess the level of hospital preparedness, scientific literature on its application in real life is scarce. This study aimed to investigate the use of the Hospital Safety Index to assess disaster preparedness in healthcare facilities. A retrospective, qualitative study employing semi-structured online interviews was conducted to gather the opinions and perspectives of professionals who have experience in applying the Hospital Safety Index. Authors of scientific publications using the Hospital Safety Index were recruited. A semi-structured interview guide was developed. It addressed different phases of data collection with the Hospital Safety Index, the challenges and facilitators of using it, and recommendations for future adaptations. Data were analysed using inductive thematic analysis. Nine participants who were from three countries (Serbia, Sri Lanka, and Indonesia) and had different professional backgrounds (medical doctors, engineers, spatial planners, etc.) participated in this study. A total of 5 themes and 15 subthemes emerged during data analysis. Most of the participants reported their reasons for choosing the Hospital Safety Index as being its comprehensiveness and the fact that it was issued by the World Health Organization. The tool appears to be very specific and allows investigators to spot details in hospitals; however, it is not easy to use, and training is highly encouraged to learn how to navigate the different components of the tool. Governmental support is a crucial facilitator for investigators to be able to enter hospitals and conduct their evaluations. Overall, the tool has a lot of potential, and it should be used to reach a broader audience, such as community members, and assess the preparedness of other facilities that can take part in the response to disasters (hotels, stadiums, schools, etc.). Nevertheless, it still needs more adaptations to be tailored to different contexts and settings.
Trace Elements in Soy-Based Beverages: A Comprehensive Study of Total Content and In Vitro Bioaccessibility
Authors: Milani Raquel Fernanda , Mauri Adriana Aparecida , Sanches Vitor Lacerda , Morgano Marcelo Antonio , Cadore Solange
Published: March 2023

Soy-based beverages are one of the most consumed plant-based beverages, which have been used as a substitute for dairy products. Soy is a source of several nutrients (vitamins, minerals, and phenolic compounds, etc.) and its consumption is usually associated with several benefits, such as the prevention of cardiovascular diseases, cancer, and osteoporosis. However, non-essential trace elements can be found in these beverages. Thus, a comprehensive study concerning trace elements Al, As, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sb, Se, Sn, Sr, and Zn in soy-based beverages was proposed. In vitro digestion allowed to simulate the gastrointestinal juice (bioaccessibility) and the Caco-2 cells culture model was applied for the bioavailability assay. Trace elements measures were performed by inductively coupled plasma optical emission spectrometry (ICP OES). Multivariate analysis classified soy-based beverages according to their soy source (isolate protein, hydrosoluble extract, and beans); Al, Cu, Fe, Mn, Sr, Se, and Zn bioaccessible fractions corresponded to approximately 40%-80% of their total content, and soy-based beverages were found to be a good Fe, Se, and Zn source. However, our results showed risk exposure assessment from daily consumption of one glass of soy-based beverage can contribute to 3.5% and 0.9% of Al Provisional Tolerable Weekly Intake (PTWI) for children and adults, respectively.
Physiological Effects of a Garden Plant Smellscape from the Perspective of Perceptual Interaction
Authors: Zhang Xinguo , Guo Jiayu , Zhang Xiaowan , Zhang Qixiang
Published: March 2023

The purpose of this study was to investigate the physiological recovery effects of olfactory, visual and olfactory–visual stimuli associated with garden plants. In a randomized controlled study design, ninety-five Chinese university students were randomly selected to be exposed to stimulus materials, namely the odor of Osmanthus fragrans and a corresponding panoramic image of a landscape featuring the plant. Physiological indexes were measured by the VISHEEW multiparameter biofeedback instrument and a NeuroSky EEG tester in a virtual simulation laboratory. The results showed the following: (1) In the olfactory stimulation group, from before to during exposure to the stimuli, the subjects’ diastolic blood pressure (DBP) (ΔDBP = 4.37 ± 1.69 mmHg, p < 0.05) and pulse pressure (PP) values increased (ΔPP = −4.56 ± 1.24 mmHg, p < 0.05), while their pulse (p) values decreased (ΔP = −2.34 ± 1.16 bmp, p < 0.05) significantly. When compared to the control group, only the amplitudes of α and β brainwaves increased significantly (Δα = 0.37 ± 2.09 µV, Δβ = 0.34 ± 1.01 µV, p < 0.05). (2) In the visual stimulation group, the amplitudes of skin conductance (SC) (ΔSC = 0.19 ± 0.01 µΩ, p < 0.05), α brainwaves (Δα = 6.2 ± 2.26 µV, p < 0.05) and β brainwaves (Δβ = 5.51 ± 1.7 µV, p < 0.05) all increased significantly relative to the control group. (3) In the olfactory–visual stimulus group, DBP (ΔDBP = 3.26 ± 0.45 mmHg, p < 0.05) values increased, and PP values decreased (ΔPP = −3.48 ± 0.33 bmp, p < 0.05) significantly from before to during exposure to the stimuli. The amplitudes of SC (ΔSC = 0.45 ± 0.34 µΩ, p < 0.05), α brainwaves (Δα = 2.28 ± 1.74 µV, p < 0.05) and β brainwaves (Δβ = 1.4 ± 0.52 µV, p < 0.05) all increased significantly relative to the control group. The results of this study show that the interaction of olfactory and visual stimuli associated with a garden plant odor landscape was able to relax and refresh the body to a certain extent, and this physiological health effect was greater with regards to the integrated response of the autonomic nervous system and central nervous system than the effect of only smelling or viewing the stimuli. In the planning and designing of plant smellscapes in garden green space, it should be ensured that plant odors and corresponding landscapes are present at the same time in order to ensure the best health effect.
Intraseason Changes in Vertical Jumps of Male Professional Basketball Players
Authors: de Pedro-Múñez Álvaro , Álvarez-Yates Tania , Serrano-Gómez Virginia , García-García Oscar
Published: March 2023

This study aimed to analyze basketball players’ jumping bhavior in the Squat Jump (SJ), Countermovement Jump (CMJ), and Free Arm Swing CMJ (CMJ Free) during a professional basketball season and check if it is modulated by the players’ specific playing position, the time played on court, and the different leagues. Fifty-three male professional basketball players were assessed in three different moments of the season through SJ, CMJ, and CMJ Free. Between the beginning of pre-season (1st assessment) and the second round of the season (3rd assessment), there was a strong increase in performance in the three jumps (SJ Height: 5.6%, η2P = 0.234, p = 0.007; CMJ Height: 5.1%, η2P = 0.177, p = 0.007; CMJ Free height: 4.11%, η2P = 0.142, p = 0.01). There was also a significantly large increase in SJ and CMJ between the 2nd and 3rd assessments and in the CMJ Free between the 1st and 2nd assessments. No significant interactions were found between jumping performance and the group factors (players’ specific playing position, time played on court, and league). In conclusion, SJ, CMJ, and CMJ Free performance strongly increases between 1st and 3rd assessment, without being influenced by the specific playing position or the minutes played per game.
Prevalence of Comorbidity and Its Effects on Sickness-Absenteeism among Brazilian Legislative Civil Servants
Authors: Sampaio Francisco Edison , Oliveira Manuel Joaquim Silva , Areosa João , Facas Emílio
Published: March 2023

Studies have shown there is an association of chronic diseases with working days lost, considering the impact of these pathologies on the levels of vulnerability of the individual’s health, with an increased risk of work disability. This article is part of a more comprehensive investigation on the sickness absenteeism of civil servants of the legislative branch in Brazil, with the purpose of determining the comorbidity index (CI) of the individuals and its correlation with days of absence from work. Sickness absenteeism was counted from the data of 37,690 medical leaves, from 2016 to 2109, involving 4149 civil servants. The self-administered comorbidity questionnaire (SCQ) was used to estimate the CI, based on the diseases or chronic health problems declared by the participants. The average number of working days lost per servant per year was 8.73 days, totaling 144,902 days of absence. The majority of the servants (65.5%) declared at least one chronic health condition. A significant association between the CI scores and working days lost was observed (r = 0.254, p-value < 0.01), thus showing that the CI may be an important predictor of sickness absenteeism. Chronic diseases or health problems are a characteristic of the general population, often affecting working capacity.
A Pilot Randomised Controlled Trial to Increase the Sustainment of an Indoor–Outdoor-Free-Play Program in Early Childhood Education and Care Services: A Study Protocol for the Sustaining Play, Sustaining Health (SPSH) Trial
Authors: Imad Noor , Pearson Nicole , Hall Alix , Shoesmith Adam , Nathan Nicole , Giles Luke , Grady Alice , Yoong Serene
Published: March 2023

Early Childhood Education and Care (ECEC) settings are important environments to support children’s physical activity (PA). In 2021, COVID-19 regulations recommended the provision of indoor–outdoor free-play programs in ECEC settings to reduce the transmission of COVID-19, resulting in an increased uptake of this practice. As the context has since changed, research suggests that ECEC services could cease the implementation of these practices. Therefore, this pilot randomised controlled trial (RCT) aims to examine the feasibility, acceptability, and impact of a sustainment strategy to ensure the ongoing implementation (sustainment) of ECEC-delivered indoor–outdoor free-play programs. Twenty ECEC services located in New South Wales, Australia that have implemented indoor–outdoor free-play programs since the release of COVID-19 guidelines will be recruited. The services will be randomly allocated either the sustainment strategy or usual care. The “Sustaining Play, Sustaining Health” program consists of eight strategies, developed to address key barriers against and facilitators of sustainment informed by the Integrated Sustainability Framework. The outcomes will be assessed via internal project records, staff surveys, and a self-reported measure of free play. This study will provide important data to support the performance of a fully powered trial within Australian ECEC settings and to inform the development of future sustainment strategies.
Effect of the N-hexanoyl-L-homoserine Lactone on the Carbon Fixation Capacity of the Algae–Bacteria System
Authors: Liao Lei , Chen Bin , Deng Kaikai , He Qiang , Lin Guijiao , Guo Jinsong , Yan Peng
Published: March 2023

Algae–bacteria systems are used widely in wastewater treatment. N-hexanoyl-L-homoserine lactone (AHL) plays an important role in algal-bacteria communication. However, little study has been conducted on the ability of AHLs to regulate algal metabolism and the carbon fixation ability, especially in algae–bacteria system. In this study, we used the Microcystis aeruginosa + Staphylococcus ureilyticus strain as a algae–bacteria system. The results showed that 10 ng/L C6-HSL effectively increased the chlorophyll-a (Chl-a) concentration and carbon fixation enzyme activities in the algae–bacteria group and algae group, in which Chl-a, carbonic anhydrase activity, and Rubisco enzyme increased by 40% and 21%, 56.4% and 137.65%, and 66.6% and 10.2%, respectively, in the algae–bacteria group and algae group, respectively. The carbon dioxide concentration mechanism (CCM) model showed that C6-HSL increased the carbon fixation rate of the algae–bacteria group by increasing the CO2 transport rate in the water and the intracellular CO2 concentration. Furthermore, the addition of C6-HSL promoted the synthesis and secretion of the organic matter of algae, which provided biogenic substances for bacteria in the system. This influenced the metabolic pathways and products of bacteria and finally fed back to the algae. This study provided a strategy to enhance the carbon fixation rate of algae–bacteria consortium based on quorum sensing.
Male Involvement in Family Planning Decisions in Malawi and Tanzania: What Are the Determinants?
Authors: Osuafor Godswill Nwabuisi , Akokuwebe Monica Ewomazino , Idemudia Erhabor Sunday
Published: March 2023

The participation of males in joint spousal decisions is urgently needed in achieving the fundamental indicators of reproductive health. The low involvement of males in family planning (FP) decision-making is a major determining factor in low FP usage in Malawi and Tanzania. Despite this, there are inconsistent findings regarding the extent of male involvement and the determinants that aid male participation in FP decisions in these two countries. The objective of this study was to assess the prevalence of male involvement in FP decisions and its associated determinants within the household context in Malawi and Tanzania. We used data from the 2015–2016 Malawi and Tanzania Demographic and Health Surveys (DHSs) to examine the prevalence and the determinants inhibiting male involvement in FP decisions. The total sample size of 7478 from Malawi and 3514 males from Tanzania aged 15–54 years was employed in the analysis by STATA version 17. Descriptive (graphs, tables and means), bi-variate (chi-square) and logistic regression analyses (unadjusted (U) and adjusted odds ratio (AOR)) were performed to identify the determinants associated with male involvement in FP decisions. The mean age of respondents in Malawi was 32 years (±8 SD) and in Tanzania, 36 years (±6 SD), with the prevalence of male involvement in FP decisions being 53.0% in Malawi and 26.6% in Tanzania. Being aged 35–44 years [AOR = 1.81; 95% CI: 1.59–2.05] and 45–54 years [AOR = 1.43; 95% CI: 1.22–1.67], educated (secondary/higher) [AOR = 1.62; 95% CI: 1.31–1.99], having access to media information [AOR = 1.35; 95% CI: 1.21–1.51] and having a female head of household [AOR = 1.79; 95% CI: 1.70–1.90] were determinant factors of male involvement in FP decisions in Malawi. Primary education [AOR = 1.94; 95% CI: 1.39–2.72], having a middle wealth index ranking [AOR = 1.46; 95% CI: 1.17–1.81], being married [AOR = 1.62; 95% CI: 1.38–1.90] and working [AOR = 2.86; 95% CI: 2.10–3.88] were higher predictors of male involvement in FP decisions in Tanzania. Increasing the role of males in FP decisions and involvement in FP utilization may improve uptake and continuity of FP usage. Therefore, the findings from this cross-sectional study will support redesigning the ineffective strategic FP programs that accommodate socio-demographic determinants that may increase the likelihood of male involvement in FP decisions, especially in the grassroots settings in Malawi and Tanzania.
Generating Gridded Gross Domestic Product Data for China Using Geographically Weighted Ensemble Learning
Authors: Xu Zekun , Wang Yu , Sun Guihou , Chen Yuehong , Ma Qiang , Zhang Xiaoxiang
Published: March 2023

Gridded gross domestic product (GDP) data are a crucial land surface parameter for many geoscience applications. Recently, machine learning approaches have become powerful tools in generating gridded GDP data. However, most machine learning approaches for gridded GDP estimation seldom consider the geographical properties of input variables. Therefore, in this study, a geographically weighted stacking ensemble learning approach was developed to generate gridded GDP data. Three algorithms—random forest, XGBoost, and LightGBM—were used as base models, and the linear regression in stacking ensemble learning was replaced by geographically weighted regression to locally fuse the three predictions. A case study was conducted in China to demonstrate the effectiveness of the proposed approach. The results showed that the proposed GDP downscaling approach outperformed the three base models and traditional stacking ensemble learning. Meanwhile, it had good predictive power on county-level GDP test data with R2 of 0.894, 0.976, and 0.976 for the primary, secondary, and tertiary sectors, respectively. Moreover, the predicted 1 km gridded GDP data had a high accuracy (R2 = 0.787) when evaluated by town-level GDP data. Hence, the proposed GDP downscaling approach provides a valuable option for generating gridded GDP data. The generated 1 km gridded GDP data of China from 2020 are of great significance for other applications.
Iron Deficiency and Nephrotoxic Heavy Metals: A Dangerous Interplay?
Authors: Rawee Pien , Kremer Daan , Nolte Ilja M. , Leuvenink Henri G. D. , Touw Daan J. , De Borst Martin H. , Bakker Stephan J. L. , Hanudel Mark R. , Eisenga Michele F.
Published: March 2023

Heavy metals are common in our environment, and all individuals are exposed to them to some extent. These toxic metals have several harmful effects on the body, including the kidney, which is a very sensitive organ. Indeed, heavy metal exposure has been linked to an increased risk of chronic kidney disease (CKD) and its progression, which may be explained by the well-established nephrotoxic effects of these metals. In this hypothesis and narrative literature review, we will shed light on the potential role that another highly common problem in patients with CKD, iron deficiency, may play in the damaging effects of heavy metal exposure in this patient group. Iron deficiency has previously been linked with an increased uptake of heavy metals in the intestine due to the upregulation of iron receptors that also take up other metals. Furthermore, recent research suggests a role of iron deficiency in the retention of heavy metals in the kidney. Therefore, we hypothesize that iron deficiency plays a crucial role in the damaging effects of heavy metal exposure in patients with CKD and that iron supplementation might be a strategy to combat these detrimental processes.
Thermochemistry, Tautomerism, and Thermal Stability of 5,7-Dinitrobenzotriazoles
Authors: Melnikov Igor N. , Kiselev Vitaly G. , Dalinger Igor L. , Starosotnikov Alexey M. , Muravyev Nikita V. , Pivkina Alla N.
Published: March 2023

Nitro derivatives of benzotriazoles are safe energetic materials with remarkable thermal stability. In the present study, we report on the kinetics and mechanism of thermal decomposition for 5,7-dinitrobenzotriazole (DBT) and 4-amino-5,7-dinitrobenzotriazole (ADBT). The pressure differential scanning calorimetry was employed to study the decomposition kinetics of DBT experimentally because the measurements under atmospheric pressure are disturbed by competing evaporation. The thermolysis of DBT in the melt is described by a kinetic scheme with two global reactions. The first stage is a strong autocatalytic process that includes the first-order reaction (Ea1I = 173.9 ± 0.9 kJ mol−1, log(A1I/s−1) = 12.82 ± 0.09) and the catalytic reaction of the second order with Ea2I = 136.5 ± 0.8 kJ mol−1, log(A2I/s−1) = 11.04 ± 0.07. The experimental study was complemented by predictive quantum chemical calculations (DLPNO-CCSD(T)). The calculations reveal that the 1H tautomer is the most energetically preferable form for both DBT and ADBT. Theory suggests the same decomposition mechanisms for DBT and ADBT, with the most favorable channels being nitro-nitrite isomerization and C–NO2 bond cleavage. The former channel has lower activation barriers (267 and 276 kJ mol−1 for DBT and ADBT, respectively) and dominates at lower temperatures. At the same time, due to the higher preexponential factor, the radical bond cleavage, with reaction enthalpies of 298 and 320 kJ mol−1, dominates in the experimental temperature range for both DBT and ADBT. In line with the theoretical predictions of C–NO2 bond energies, ADBT is more thermally stable than DBT. We also determined a reliable and mutually consistent set of thermochemical values for DBT and ADBT by combining the theoretically calculated (W1-F12 multilevel procedure) gas-phase enthalpies of formation and experimentally measured sublimation enthalpies.
Biomarkers of Migraine: An Integrated Evaluation of Preclinical and Clinical Findings
Authors: Demartini Chiara , Francavilla Miriam , Zanaboni Anna Maria , Facchetti Sara , De Icco Roberto , Martinelli Daniele , Allena Marta , Greco Rosaria , Tassorelli Cristina
Published: March 2023

In recent years, numerous efforts have been made to identify reliable biomarkers useful in migraine diagnosis and progression or associated with the response to a specific treatment. The purpose of this review is to summarize the alleged diagnostic and therapeutic migraine biomarkers found in biofluids and to discuss their role in the pathogenesis of the disease. We included the most informative data from clinical or preclinical studies, with a particular emphasis on calcitonin gene-related peptide (CGRP), cytokines, endocannabinoids, and other biomolecules, the majority of which are related to the inflammatory aspects and mechanisms of migraine, as well as other actors that play a role in the disease. The potential issues affecting biomarker analysis are also discussed, such as how to deal with bias and confounding data. CGRP and other biological factors associated with the trigeminovascular system may offer intriguing and novel precision medicine opportunities, although the biological stability of the samples used, as well as the effects of the confounding role of age, gender, diet, and metabolic factors should be considered.
Ligand-Induced Activation of GPR110 (ADGRF1) to Improve Visual Function Impaired by Optic Nerve Injury
Authors: Kwon Heung-Sun , Kevala Karl , Qian Haohua , Abu-Asab Mones , Patnaik Samarjit , Marugan Juan , Kim Hee-Yong
Published: March 2023

It is extremely difficult to achieve functional recovery after axonal injury in the adult central nervous system. The activation of G-protein coupled receptor 110 (GPR110, ADGRF1) has been shown to stimulate neurite extension in developing neurons and after axonal injury in adult mice. Here, we demonstrate that GPR110 activation partially restores visual function impaired by optic nerve injury in adult mice. Intravitreal injection of GPR110 ligands, synaptamide and its stable analogue dimethylsynaptamide (A8) after optic nerve crush significantly reduced axonal degeneration and improved axonal integrity and visual function in wild-type but not gpr110 knockout mice. The retina obtained from the injured mice treated with GPR110 ligands also showed a significant reduction in the crush-induced loss of retinal ganglion cells. Our data suggest that targeting GPR110 may be a viable strategy for functional recovery after optic nerve injury.
Bottom-Up Proteomics: Advancements in Sample Preparation
Authors: Duong Van-An , Lee Hookeun
Published: March 2023

Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom-up proteomic workflows consist of the following three major steps: sample preparation, LC–MS/MS analysis, and data analysis. LC–MS/MS and data analysis techniques have been intensively developed, whereas sample preparation, a laborious process, remains a difficult task and the main challenge in different applications. Sample preparation is a crucial stage that affects the overall efficiency of a proteomic study; however, it is prone to errors and has low reproducibility and throughput. In-solution digestion and filter-aided sample preparation are the typical and widely used methods. In the past decade, novel methods to improve and facilitate the entire sample preparation process or integrate sample preparation and fractionation have been reported to reduce time, increase throughput, and improve reproducibility. In this review, we have outlined the current methods used for sample preparation in proteomics, including on-membrane digestion, bead-based digestion, immobilized enzymatic digestion, and suspension trapping. Additionally, we have summarized and discussed current devices and methods for integrating different steps of sample preparation and peptide fractionation.
Analysis of the Electron Density of a Water Molecule Encapsulated by Two Cholic Acid Residues
Authors: Vázquez-Tato María Pilar , Seijas Julio A. , Meijide Francisco , de Frutos Santiago , Vázquez Tato José
Published: March 2023

Cholic acid is a trihydroxy bile acid with a nice peculiarity: the average distance between the oxygen atoms (O7 and O12) of the hydroxy groups located at C7 and C12 carbon atoms is 4.5 Å, a value which perfectly matches with the O/O tetrahedral edge distance in Ih ice. In the solid phase, they are involved in the formation of hydrogen bonds with other cholic acid units and solvents. This fact was satisfactorily used for designing a cholic dimer which encapsulates one single water molecule between two cholic residues, its oxygen atom (Ow) being exactly located at the centroid of a distorted tetrahedron formed by the four steroid hydroxy groups. The water molecule participates in four hydrogen bonds, with the water simultaneously being an acceptor from the 2 O12 (hydrogen lengths are 2.177 Å and 2.114 Å) and a donor towards the 2 O7 (hydrogen bond lengths are 1.866 Å and 1.920 Å). These facts suggest that this system can be a nice model for the theoretical study of the formation of ice-like structures. These are frequently proposed to describe the water structure found in a plethora of systems (water interfaces, metal complexes, solubilized hydrophobic species, proteins, and confined carbon nanotubes). The above tetrahedral structure is proposed as a reference model for those systems, and the results obtained from the application of the atoms in molecules theory are presented here. Furthermore, the structure of the whole system allows a division into two interesting subsystems in which water is the acceptor of one hydrogen bond and the donor of another. The analysis of the calculated electron density is performed through its gradient vector and the Laplacian. The calculation of the complexation energy used correction of the basis set superposition error (BSSE) with the counterpoise method. As expected, four critical points located in the H…O bond paths were identified. All calculated parameters obey the proposed criteria for hydrogen bonds. The total energy for the interaction in the tetrahedral structure is 54.29 kJ/mol, while the summation obtained of the two independent subsystems and the one between the alkyl rings without water is only 2.5 kJ/mol higher. This concordance, together with the calculated values for the electron density, the Laplacian of the electron density, and the lengths of the oxygen atom and the hydrogen atom (involved in the formation of each hydrogen bond) to the hydrogen bond critical point, suggests that each pair of hydrogen bonds can be considered independent of each other.
The Role of Regulated Programmed Cell Death in Osteoarthritis: From Pathogenesis to Therapy
Authors: Liu Suqing , Pan Yurong , Li Ting , Zou Mi , Liu Wenji , Li Qingqing , Wan Huan , Peng Jie , Hao Liang
Published: March 2023

Osteoarthritis (OA) is a worldwide chronic disease that can cause severe inflammation to damage the surrounding tissue and cartilage. There are many different factors that can lead to osteoarthritis, but abnormally progressed programmed cell death is one of the most important risk factors that can induce osteoarthritis. Prior studies have demonstrated that programmed cell death, including apoptosis, pyroptosis, necroptosis, ferroptosis, autophagy, and cuproptosis, has a great connection with osteoarthritis. In this paper, we review the role of different types of programmed cell death in the generation and development of OA and how the different signal pathways modulate the different cell death to regulate the development of OA. Additionally, this review provides new insights into the radical treatment of osteoarthritis rather than conservative treatment, such as anti-inflammation drugs or surgical operation.
Culture Conditions for Human Induced Pluripotent Stem Cell-Derived Schwann Cells: A Two-Centre Study
Authors: Huang Zhong , Powell Rebecca , Kankowski Svenja , Phillips James B. , Haastert-Talini Kirsten
Published: March 2023

Adult human Schwann cells represent a relevant tool for studying peripheral neuropathies and developing regenerative therapies to treat nerve damage. Primary adult human Schwann cells are, however, difficult to obtain and challenging to propagate in culture. One potential solution is to generate Schwann cells from human induced pluripotent stem cells (hiPSCs). Previously published protocols, however, in our hands did not deliver sufficient viable cell numbers of hiPSC-derived Schwann cells (hiPSC-SCs). We present here, two modified protocols from two collaborating laboratories that overcome these challenges. With this, we also identified the relevant parameters to be specifically considered in any proposed differentiation protocol. Furthermore, we are, to our knowledge, the first to directly compare hiPSC-SCs to primary adult human Schwann cells using immunocytochemistry and RT-qPCR. We conclude the type of coating to be important during the differentiation process from Schwann cell precursor cells or immature Schwann cells to definitive Schwann cells, as well as the amounts of glucose in the specific differentiation medium to be crucial for increasing its efficiency and the final yield of viable hiPSC-SCs. Our hiPSC-SCs further displayed high similarity to primary adult human Schwann cells.
The Effect of Aldosterone on Cardiorenal and Metabolic Systems
Authors: Otsuka Hiromasa , Abe Masanori , Kobayashi Hiroki
Published: March 2023

Aldosterone, a vital hormone of the human body, has various pathophysiological roles. The excess of aldosterone, also known as primary aldosteronism, is the most common secondary cause of hypertension. Primary aldosteronism is associated with an increased risk of cardiovascular disease and kidney dysfunction compared to essential hypertension. Excess aldosterone can lead to harmful metabolic and other pathophysiological alterations, as well as cause inflammatory, oxidative, and fibrotic effects in the heart, kidney, and blood vessels. These alterations can result in coronary artery disease, including ischemia and myocardial infarction, left ventricular hypertrophy, heart failure, arterial fibrillation, intracarotid intima thickening, cerebrovascular disease, and chronic kidney disease. Thus, aldosterone affects several tissues, especially in the cardiovascular system, and the metabolic and pathophysiological alterations are related to severe diseases. Therefore, understanding the effects of aldosterone on the body is important for health maintenance in hypertensive patients. In this review, we focus on currently available evidence regarding the role of aldosterone in alterations of the cardiovascular and renal systems. We also describe the risk of cardiovascular events and renal dysfunction in hyperaldosteronism.
COVID-19-Induced Myocarditis: Pathophysiological Roles of ACE2 and Toll-like Receptors
Authors: Pannucci Patrizia , Jefferson Sophie R. , Hampshire Jonathan , Cooper Samantha L. , Hill Stephen J. , Woolard Jeanette
Published: March 2023

The clinical manifestations of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection responsible for coronavirus disease 2019 (COVID-19) commonly include dyspnoea and fatigue, and they primarily involve the lungs. However, extra-pulmonary organ dysfunctions, particularly affecting the cardiovascular system, have also been observed following COVID-19 infection. In this context, several cardiac complications have been reported, including hypertension, thromboembolism, arrythmia and heart failure, with myocardial injury and myocarditis being the most frequent. These secondary myocardial inflammatory responses appear to be associated with a poorer disease course and increased mortality in patients with severe COVID-19. In addition, numerous episodes of myocarditis have been reported as a complication of COVID-19 mRNA vaccinations, especially in young adult males. Changes in the cell surface expression of angiotensin-converting enzyme 2 (ACE2) and direct injury to cardiomyocytes resulting from exaggerated immune responses to COVID-19 are just some of the mechanisms that may explain the pathogenesis of COVID-19-induced myocarditis. Here, we review the pathophysiological mechanisms underlying myocarditis associated with COVID-19 infection, with a particular focus on the involvement of ACE2 and Toll-like receptors (TLRs).
Modification of the TRP Channel TRPA1 as a Relevant Factor in Migraine-Related Intracranial Hypersensitivity
Authors: Masood Thannoon , Lakatos Szandra , Rosta Judit
Published: March 2023

Recently, the transient receptor potential ankyrin 1 (TRPA1) has gained more attention in migraine-related research. The involvement of the TRPA1 receptor in migraine headaches is proposed by the fact that TRPA1 may be a target of some migraine-triggering factors. Although it is doubtful that activation of TRPA1 alone is sufficient to induce pain, behavioral studies have demonstrated that TRPA1 is involved in injury- and inflammation-induced hypersensitivity. Here, we review the functional relevance of TRPA1 in headaches and its therapeutic potential, mainly focusing on its role in the development of hypersensitivity, referring to its altered expression in pathological conditions, and its functional interaction with other TRP channels.
Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research
Authors: Skopek Rafał , Palusińska Małgorzata , Kaczor-Keller Katarzyna , Pingwara Rafał , Papierniak-Wyglądała Anna , Schenk Tino , Lewicki Sławomir , Zelent Artur , Szymański Łukasz
Published: March 2023

Immortalized cell lines are widely used in vitro tools in oncology and hematology research. While these cell lines represent artificial systems and may accumulate genetic aberrations with each passage, they are still considered valuable models for pilot, preliminary, and screening studies. Despite their limitations, cell lines are cost-effective and provide repeatable and comparable results. Choosing the appropriate cell line for acute myeloid leukemia (AML) research is crucial for obtaining reliable and relevant results. Several factors should be considered when selecting a cell line for AML research, such as specific markers and genetic abnormalities associated with different subtypes of AML. It is also essential to evaluate the karyotype and mutational profile of the cell line, as these can influence the behavior and response to the treatment of the cells. In this review, we evaluate immortalized AML cell lines and discuss the issues surrounding them concerning the revised World Health Organization and the French–American–British classifications.
Chronic Pain-Associated Cardiovascular Disease: The Role of Sympathetic Nerve Activity
Authors: Reynolds Christian A. , Minic Zeljka
Published: March 2023

Chronic pain affects many people world-wide, and this number is continuously increasing. There is a clear link between chronic pain and the development of cardiovascular disease through activation of the sympathetic nervous system. The purpose of this review is to provide evidence from the literature that highlights the direct relationship between sympathetic nervous system dysfunction and chronic pain. We hypothesize that maladaptive changes within a common neural network regulating the sympathetic nervous system and pain perception contribute to sympathetic overactivation and cardiovascular disease in the setting of chronic pain. We review clinical evidence and highlight the basic neurocircuitry linking the sympathetic and nociceptive networks and the overlap between the neural networks controlling the two.
Comprehensive Genome-Wide Analyses of Poplar R2R3-MYB Transcription Factors and Tissue-Specific Expression Patterns under Drought Stress
Authors: Zhang Xueli , Wang Haoran , Chen Ying , Huang Minren , Zhu Sheng
Published: March 2023

R2R3-type MYB transcription factors are implicated in drought stress, which is a primary factor limiting the growth and development of woody plants. The identification of R2R3-MYB genes in the Populus trichocarpa genome has been previously reported. Nevertheless, the diversity and complexity of the conserved domain of the MYB gene caused inconsistencies in these identification results. There is still a lack of drought-responsive expression patterns and functional studies of R2R3-MYB transcription factors in Populus species. In this study, we identified a total of 210 R2R3-MYB genes in the P. trichocarpa genome, of which 207 genes were unevenly distributed across all 19 chromosomes. These poplar R2R3-MYB genes were phylogenetically divided into 23 subgroups. Collinear analysis demonstrated that the poplar R2R3-MYB genes underwent rapid expansion and that whole-genome duplication events were a dominant factor in the process of rapid gene expansion. Subcellular localization assays indicated that poplar R2R3-MYB TFs mainly played a transcriptional regulatory role in the nucleus. Ten R2R3-MYB genes were cloned from P. deltoides × P. euramericana cv. Nanlin895, and their expression patterns were tissue-specific. A majority of the genes showed similar drought-responsive expression patterns in two out of three tissues. This study provides a valid cue for further functional characterization of drought-responsive R2R3-MYB genes in poplar and provides support for the development of new poplar genotypes with elevated drought tolerance.
Application of Pyrolysis for the Evaluation of Organic Compounds in Medical Plastic Waste Generated in the City of Cartagena-Colombia Applying TG-GC/MS
Authors: Hernandez-Fernandez Joaquín , Lambis Henry , Reyes Ricardo Vivas
Published: March 2023

In this study, the thermal degradation and pyrolysis of hospital plastic waste consisting of polyethylene (PE), polystyrene (PS), and polypropylene (PP) were investigated using TG-GC/MS. The identified molecules with the functional groups of alkanes, alkenes, alkynes, alcohols, aromatics, phenols, CO and CO2 were found in the gas stream from pyrolysis and oxidation, and are chemical structures with derivatives of aromatic rings. They are mainly related to the degradation of PS hospital waste, and the alkanes and alkenes groups originate mainly from PP and PE-based medical waste. The pyrolysis of this hospital waste did not show the presence of derivatives of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, which is an advantage over classical incineration methodologies. CO, CO2, phenol, acetic acid and benzoic acid concentrations were higher in the gases from the oxidative degradation than in those generated in the pyrolysis with helium. In this article, we propose different pathways of reaction mechanisms that allow us to explain the presence of molecules with other functional groups, such as alkanes, alkenes, carboxylic acids, alcohols, aromatics and permanent gases.