bluuebunny/crossref_metadata_2025 huggingface dataset:
Research progress of moyamoya disease combined with renovascular hypertension
Authors: Liu Erheng , Zhao Heng , Liu Chengyuan , Tan Xueyi , Luo Chao , Yang Shuaifeng
Published: August 2022

Moyamoya disease (MMD) is an idiopathic cerebrovascular disease which was first described by Suzuki and Takaku in 1969. Moyamoya disease is a non-atherosclerotic cerebrovascular structural disorder. MMD has been found all over the world, especially in Japan, Korea, and China. In recent years, many reports pointed out that the changes of vascular stenosis in patients with moyamoya disease occurred not only in intracranial vessels, but also in extracranial vessels, especially the changes of renal artery. Renovascular hypertension (RVH) is considered to be one of the important causes of hypertension in patients with moyamoya disease. The pathogenesis of moyamoya disease combined with renovascular hypertension is still unclear, and the selection of treatment has not yet reached a consensus. This article reviews the latest research progress in epidemiology, RNF213 gene, pathomorphology, clinical characteristics and treatment of moyamoya disease combined with renovascular hypertension, in order to provide reference for clinical workers.
Distributed Conflict Resolution at High Traffic Densities with Reinforcement Learning
Authors: Ribeiro Marta , Ellerbroek Joost , Hoekstra Jacco
Published: August 2022

Future operations involving drones are expected to result in traffic densities that are orders of magnitude higher than any observed in manned aviation. Current geometric conflict resolution (CR) methods have proven to be very efficient at relatively moderate densities. However, at higher densities, performance is hindered by the unpredictable emergent behaviour from neighbouring aircraft. Reinforcement learning (RL) techniques are often capable of identifying emerging patterns through training in the environment. Although some work has started introducing RL to resolve conflicts and ensure separation between aircraft, it is not clear how to employ these methods with a higher number of aircraft, and whether these can compare to or even surpass the performance of current CR geometric methods. In this work, we employ an RL method for distributed conflict resolution; the method is completely responsible for guaranteeing minimum separation of all aircraft during operation. Two different action formulations are tested: (1) where the RL method controls heading, and speed variation; (2) where the RL method controls heading, speed, and altitude variation. The final safety values are directly compared to a state-of-the-art distributed CR algorithm, the Modified Voltage Potential (MVP) method. Although, overall, the RL method is not as efficient as MVP in reducing the total number of losses of minimum separation, its actions help identify favourable patterns to avoid conflicts. The RL method has a more preventive behaviour, defending in advance against nearby neighbouring aircraft not yet in conflict, and head-on conflicts while intruders are still far away.
Preliminary Sizing of the Electrical Motor and Housing of Electromechanical Actuators Applied on the Primary Flight Control System of Unmanned Helicopters
Authors: Roussel Jeremy , Budinger Marc , Ruet Laurent
Published: August 2022

Helicopter dronization is expanding, for example, the VSR700 project. This leads to the integration of electromechanical actuators (EMAs) into the primary flight control system (PFCS). The PFCS is in charge of controlling the helicopter flight over its four axes (roll, pitch, yaw, and vertical). It controls the blade pitch thanks to mechanical kinematics and actuators. For more than 60 years, the actuators have been conventionally using the hydraulic technology. The EMA technology introduction involves the reconsideration of the design practices. Indeed, an EMA is multidisciplinary. Each of its components introduces new design drivers and new inherent technological imperfections (friction, inertia, and losses). This paper presents a methodology to specify and pre-design critical EMAs. The description will be focused on two components: the electrical motor and the housing. This includes a data-driven specification, scaling laws for motor losses estimation, and surrogate modeling for the housing vibratory sizing. The tools are finally applied to two study cases. The first case considers two potential redundant topologies of actuation. The housing sizing shows that one prevails on the other. The second case considers the actuators of helicopter rotors. The electrical motor sizing highlights the importance of designing two separate actuators.
A Fault Diagnosis Method for a Differential Inverse Gearbox of a Crawler Combine Harvester Based on Order Analysis
Authors: Li Yaoming , Liu Yanbin , Ji Kuizhou , Zhu Ruiheng
Published: August 2022

The single HST (Hydro Static Transmission) mechanical differential transmission gearbox of crawler combine harvester is subjected to the impact of alternating loads in the field operation, resulting in its fatigue failure. The traditional durability fatigue test can improve the fault-free working time of machinery, but it is not suitable for agricultural machinery with time-varying load frequency bandwidth and stress amplitude. Therefore, in this paper, a fault diagnosis method based on order analysis was proposed considering the comprehensive influence of load amplitude and frequency on the fatigue life of gearbox. The location and the corresponding type of fault were found by comparing the spectral line peak changed before and after. Then, the test verification was carried out on the gearbox assembly fatigue test bench according to the compiled load spectrum. The results show that the analysis results of the fault diagnosis method based on order analysis were consistent with the unpacking inspection, which had reference significance for the research on the fault diagnosis method of the crawler combined harvester gearbox.
Optimization and Experiment of a Disturbance-Assisted Seed Filling High-Speed Vacuum Seed-Metering Device Based on DEM-CFD
Authors: Liu Rui , Liu Zhongjun , Zhao Jiale , Lu Qi , Liu Lijing , Li Yinghang
Published: August 2022

In disturbance-assisted seed filling vacuum seed-metering devices, the suction hole causes difficulty in quickly and stably sucking seeds when used for high-speed seeding and the large working negative pressure demand is used. To address this difficulty, this study’s authors designed a seed disk hole with a variable cross-section structure, and the groove depth, which has the function of assisting seed filling, was optimized. Using the combined method of Fluent numerical analysis and an orthogonal experiment, the influence of the shape, inlet diameter, and length of the seed disk hole on the pressure difference of the seed disk hole was analyzed. The working process of the vacuum seed meter was simulated and analyzed by the Discrete Element Method and Computational Fluid Dynamics coupling method. It was found that with the increase of the diameter of the seed disk hole, the pressure difference decreased, and the length of the seed disk hole had little effect on the pressure difference. The best diameter of the seed disk hole was 5.4 mm, and the length was 5 mm. It was found that the pressurization effect of the arc-shaped seed disk hole is better. With the increase of groove depth, the leakage rate shows a trend of first decreasing and then increasing, which was determined to be 1.5 mm. The verification experiment proved that the working performance of the optimized seed metering device is better than that of the original one. The results show that when the working negative pressure was 4 kPa and the working speeds were 8~14 km·h−1, the qualified rate was not less than 95.0%, and the seed filling performance was relatively stable. The optimized vacuum seed metering device can be applied to high-speed seeders, while ensuring the requirements of high-speed sowing operations.
The Effects of Cover Crops on Multiple Environmental Sustainability Indicators—A Review
Authors: Rivière Clément , Béthinger Audrey , Bergez Jacques-Eric
Published: August 2022

Cover crops have been introduced in European agricultural systems due to their multiple agro-ecological services and environmental benefits, which do not necessarily affect profitability. Our paper follows a systematic literature review approach to highlight the results of 51 studies on the effects of adopting cover crops. We used a list of 41 agri-environmental sustainability indicators to present the different impacts of cover crops in European pedoclimatic situations. Herein, we review the positive effects of cover crops on agri-environmental sustainability (e.g., reduced soil erosion and nitrate leaching, higher carbon sequestration and soil quality, biodiversity enhancement, and reduced mineral fertilizer requirement), but also the more variable effects associated with the use of cover crops (e.g., management and interest for farm economics, nutrient and water competition with cash crops, and improved GHG balance, even if N20 emissions are slightly increased). Our review highlights these synergies among the sustainability indicators. More research data are needed on the multiple effects of cover crops in the context of diverse site-specific conditions and farm-management practices, especially between the traditional positive effects of cover crops (i.e., soil C sequestration and fertilizer savings) and their effects on climate change (i.e., GHG net balance and potential effects on global warming).
Therapeutic Effects of Crocin Alone or in Combination with Sorafenib against Hepatocellular Carcinoma: In Vivo & In Vitro Insights
Authors: Abdu Suzan , Juaid Nouf , Amin Amr , Moulay Mohamed , Miled Nabil
Published: August 2022

This study investigated the therapeutic effects of the phytochemical crocin alone or in combination with sorafenib both in rats chemically induced with hepatocellular carcinoma (HCC) and in human liver cancer cell line (HepG2). Male rats were randomly divided into five groups, namely, control group, HCC induced group, and groups treated with sorafenib, crocin or both crocin and sorafenib. HCC was induced in rats with a single intraperitoneal injection of diethylnitrosamine (DEN), then 2-acetylaminofluorene (2-AAF). The HCC-induced rats showed a significant decrease in body weight compared to animals treated with either or both examined drugs. Serum inflammatory markers (C-reactive protein (CRP); interleukin-6 (IL-6); lactate dehydrogenase (LDH), and oxidative stress markers were significantly increased in the HCC group and were restored upon treatment with either or both of therapeutic molecules. Morphologically, the HCC-induced rats manifested most histopathological features of liver cancer. Treatment with either or both of crocin and sorafenib successfully restored normal liver architecture. The expression of key genes involved in carcinogenesis (TNFα, p53, VEGF and NF-κB) was highly augmented upon HCC induction and was attenuated post-treatment with either or both examined drugs. Treatment with both crocin and sorafenib improved the histopathological and inflammation parameters as compared to single treatments. The in vivo anti-cancer effects of crocin and/or sorafenib were supported by their respective cytotoxicity on HepG2 cells. Crocin and sorafenib displayed an anti-tumor synergetic effect on HepG2 cells. The present findings demonstrated that a treatment regimen with crocin and sorafenib reduced liver toxicity, impeded HCC development, and improved the liver functions.
Antioxidant Capacities and Polyphenol Contents of Kombucha Beverages Based on Vine Tea and Sweet Tea
Authors: Saimaiti Adila , Huang Si-Yu , Xiong Ruo-Gu , Wu Si-Xia , Zhou Dan-Dan , Yang Zhi-Jun , Luo Min , Gan Ren-You , Li Hua-Bin
Published: August 2022

Kombucha beverage is commonly prepared by black tea infusion fermentation without tea residues, and possesses various health benefits. In this paper, kombucha beverages of two non-Camellia sinensis teas, including vine tea (Ampelopsisgrossedentata) and sweet tea (Rubus suavissimus), were studied for the first time. The antioxidant activities and polyphenol contents of kombucha beverages were evaluated by ferric-reducing antioxidant power assay, Trolox equivalent antioxidant capacity assay, and Folin-Ciocalteu method, respectively. In addition, effects of tea residues on antioxidant capacities of kombucha beverages were evaluated. The results showed that kombucha beverages from vine tea and sweet tea possessed strong antioxidant activities (especially vine tea kombucha), and fermentation with tea residues could significantly increase antioxidant capacities (maximum increase of 38%) and total phenolic content (maximum increase of 55%) of two kombucha beverages compared with those without tea residues. Moreover, the sensory evaluations showed that the sensory evaluation scores of kombucha with tea residues could be improved compared with those without tea residues. Furthermore, the concentrations of several bioactive components in the kombucha beverages were detected by high-performance liquid chromatography. These kombucha beverages could be used for prevention of several diseases with related of oxidative stress.
Analysis and Suppression of Self-Excited Oscillations in Pressure Servo Valve System
Authors: Huang Jian , Zhang Qiwei , Zhao Fan , Liu Xiangyu , Wang Tianyi
Published: August 2022

In this paper, the phenomenon of pressure servo valve system self-excited oscillation is studied, the dynamic model of the system is established, and the validity of the model is verified by experimental analysis. On this basis, it is concluded that the pressure oscillation with constant amplitude and nondecaying is caused by self-excited oscillation. Such a phenomenon comes from unreasonable structure design of pressure servo valve. Moreover, the factors affecting pressure oscillation are evaluated qualitatively. In order to suppress the pressure self-excited oscillation, this paper proposes a pressure servo valve structure optimization method based on weakening the positive feedback effect of the backpressure. The simulation results show that the method can effectively eliminate the system self-excited oscillation. The stability margin analysis shows that after the structure optimization, the pressure servo valve system has adequate stability margin.
Sales Forecasting, Market Analysis, and Performance Assessment for US Retail Firms: A Business Analytics Perspective
Authors: Wang Chih-Hsuan , Gu Yu-Wei
Published: August 2022

Retail firms are the best representatives of a developed country’s economic condition because they sell many of the necessary goods used for daily consumption, including food, clothes, shoes, electric appliances, and office supplies. This study presents a novel framework to help retail practitioners achieve the following goals: (1) predict sales revenues by identifying significant economic indicators, (2) estimate stable equilibriums by capturing interactive dynamics between competing firms, and (3) derive operational efficiencies and indicate required improvements by conducting performance assessments. To verify the validity of the research, data pertaining to Walmart, Costco, and Kroger are collected. Specifically, the least absolute shrinkage and selection operator (Lasso) is adopted in order to identify significant economic indicators. Consumer price index and regular wage are two common indicators that affect the the three firms’ sales numbers. In sales forecasting, support vector regression (SVR) and multivariate adaptive regression splines (MARS), respectively, perform the best in the training set and the testing set. Finally, the Lotka–Volterra model (LVM) and data envelopment analysis (DEA) are used for competitive analysis and performance assessment. A relationship of economic mutualism has been identified between the three firms. Furthermore, research findings show that Kroger performs inefficiently, though it can expect to increase sales more than the others in stable equilibriums.
An Optimized Neuro_Fuzzy Based Regression Trees for Disease Prediction Framework
Authors: Verma Ankit , Agarwal Gaurav , Gupta Amit Kumar , Sain Mangal
Published: August 2022

Nowadays, all the applications have been moved to the intelligent world for easy usage and advancements. Hence, the sensed data have been utilized in the smart medical field to analyze the disease based on the symptom and to suggest controlling the disease severity rate. However, predicting the disease severity range based on the sensed disease symptom is more complicated because of the complex and vast data. So, the present work has introduced a novel Generalized approximate Reasoning base Intelligence Control (GARIC) with Ant Lion Optimization (ALO) algorithm to forecast the disease type and measure the severity range. Here, the presence of the Ant lion fitness has afforded the finest disease classification and severity analysis results. Finally, the parameters were measured and compared with other conventional models and have recorded the finest disease prediction score and severity range. This verified the success rate of the designed model in estimating the disease severity range. In addition, the presented system helps to notify the people of medical advice by message, email, or other application.
A Secure Real-Time IoT Data Stream Based on Improved Compound Coupled Map Lattices
Authors: Wu Shyi-Tsong
Published: August 2022

A secure data stream is important for the real time communications of Internet of Things (IoT). A stream cipher with the characteristics of simple and high speed is suitable in the secure communications of IoT for its security. Some stream ciphers based on coupled map lattices (CML) were proposed. However, the original coupled map lattice shows evidence of correlation between the contiguous points. In this paper, we present an improved CML with a counter to overcome the weakness. The proposed scheme has the advantage of simplicity and suits the resource constrained IoT environment. We implement the proposed improved CML and analyze the proposed ciphers against some attacks. For the balance performance analysis, the numbers of 0 and 1 in the keystream are almost balanced, and the difference rates show that the proposed schemes have high key sensitivity. Finally, we present some experimental results of statistical random number tests for the output keystreams. Under the pass rates of the statistical test of NIST SP800-22, the proposed ciphers with improved CML, and compound CML are at least 95% and 97%, respectively. All the pass rates of the proposed stream ciphers are 100% for the statistical test of FIPS PUB 140-1.
Study on Anchorage Performance of New High-Strength Fast Anchorage Agent
Authors: Li Haifeng , Wang Kun , Dong Zizhang , Liu Tao
Published: August 2022

To achieve the efficient, rapid construction of prestressed anchor cables, in this study, through an indoor pull-out test and field basic test, we investigated the mechanical behavior, expansion, drawing performance and bond properties of a new type of high-strength, fast anchorage agent. We analyzed the influence of the water material ratio and curing time on its performance and determined the corresponding construction. It was found that the new anchoring agent could be effectively applied in field construction and achieved a compressive strength of 30 MPa within 30 h. Moreover, during the solidification process, the hydration reaction node could be reached within 5–6 h when the material expansion was and the feedback to the water–material ratio were the strongest. In addition, in the drawing process, the anchorage agent exhibited a strong bond with the reinforcement and the rock layer, and the bonding of the anchorage agent to the rock layer was greater than that of the steel bar. Therefore, in order to provide anchorage, it is necessary to increase the contact area between the steel strand and the anchorage agent.
Mechanism of Derailment at the Guardrail Position of Turnout and a Reasonable Guardrail Interval Limit
Authors: Wang Pu , Wang Shuguo , Zhao Zhenhua
Published: August 2022

Recently, derailments have been occurring frequently at the guardrail position of double-slip turnouts. In order to provide a theoretical basis for effectively avoiding derailment at the guardrail position of turnouts, this study presented a derailment mechanism, discussed the rationality of the current guardrail interval limit and its calculation method, and proposed a method for calculating the guardrail interval limit based on the vehicle running attitude and wheelset angle of attack. Based on the established calculation model, this paper calculated the guardrail interval limit for the main types of single turnouts and double-slip turnouts in China, and studied the main factors influencing the interval limit and corresponding patterns. The following conclusions were drawn. (1) In actual maintenance, it suggested to set the guardrail interval limit at 1365 mm, which can meet the requirements of the most turnouts and avoid wear incurred by vehicle–track interactions at the opening section of guardrails and derailment. (2) For single turnouts, the required guardrail interval limit becomes smaller with the decrease of the frog number, radius of the lead curve, and the increase in gage width. Further, the interval limit decreases when the endpoints of the lead curve and gage widening become closer to the buffer segment at the toe end of the guardrail. Adopting straight-track design at the restoring segment of gage widening is more likely to cause a larger twisting angle of the bogie than a curved-track design, and larger twisting angle means requiring a smaller guardrail interval limit. (3) For double-slip turnouts, the required guardrail interval limit is reduced with the decrease in the frog number and increase in gage width. The broken rail comprising the switch rail and stock rail in front of the guardrail can also enable the bogie to approach or reach the inscribing state, resulting in a negative impact not smaller than that produced by a curved rail. The larger the attack angle at the tip of the switch rail and the closer the tip of the switch rail and the end of gage widening to the buffer segment of the guardrail, the smaller the guardrail interval limit.
A Rule-Based Control Strategy of Driver Demand to Enhance Energy Efficiency of Hybrid Electric Vehicles
Authors: Huang Kuohsiu David , Nguyen Minh-Khoa , Chen Po-Tuan
Published: August 2022

In recent years, hybrid electric vehicles (HEVs) have increased significantly due to climate change and the demand for high-efficiency power sources. HEVs that combine an internal combustion engine (ICE) and an electric motor (EM) can improve the power output of the ICE and overcome the challenges of the insufficient battery life of electric vehicles. In this study, a parallel HEV with a power distribution mechanism is developed for energy saving and carbon reduction. The driver′s power demands are used as input sources, and a rule-based control strategy is used to determine the power distribution of the generator, EM, and ICE. The NEDC2000 driving cycle is used as the test benchmark to demonstrate the performance of the HEV. In comparison to ICE vehicles, the fuel efficiency of HEVs significantly improved. In addition, other parameters, including the average brake-specific fuel consumption (BSFC), brake-specific carbon monoxide emission (BSCO), and brake-specific hydrocarbons (BSHCs), were lower, which can effectively save fossil fuel and reduce air pollution.
Numerical Analysis of the Installation Process of Screw Piles Based on the FEM-SPH Coupling Method
Authors: Zhao Qingxu , Wang Yuxing , Tang Yanqin , Ren Guofeng , Qiu Zhiguo , Luo Wenhui , Ye Zilong
Published: August 2022

The installation of screw piles can cause damage to the soil, which is a dynamic and large deformation problem. In this paper, a FEM-SPH numerical model for the analysis of this large deformation problem was developed in LS-DYNA to simulate the installation process of screw piles. In addition, field installation tests of screw piles were carried out. By comparing the FEM-SPH simulation results with the experimental results and traditional FEM simulation results, it was found that the FEM-SPH coupling method has higher efficiency and accuracy in dealing with the large deformation problems caused by the installation of screw piles. Then, numerical simulations of screw piles with various parameters were conducted to analyze the differences in the installation process. The results show that the spiral pitch and pile diameter have a significant effect on the installation torque, soil stress and soil pressure during the installation process. During the installation process, the interaction between the screw pile and the soil is transferable. The installation of the screw pile will lead to the movement of soil particles in the radial and axial directions, resulting in heave damage to the shallow soil and cylindrical shear damage to the middle and deep soil. The influence range on the soil by the heave failure mechanism (HFM) and the cylindrical shear failure mechanism (CSFM) caused by the installation of screw piles is affected by pile parameters. The change in pile diameter will act on both HFM and CSFM, whereas the variation in spiral pitch will only have an influence on CSFM.
Contact Fatigue State Identification of Specimen Based on Heterogeneous Data and Evidence Theory
Authors: Chen Xiang , Liu Yu , Fu Yuan , Gu Qiancheng , Yang Yan
Published: August 2022

In order to accurately realize the contact fatigue state identification of specimen, a new method based on vibration and image heterogeneous data, as well as on D-S evidence theory, is proposed. Firstly, combined with the bearing public data set from CWRU, the vibration signal imaging methods such as SDP, GAF and GRI, as well as neural network models such as VGG16, ResNet and S-T, were compared and analyzed. It is determined that the SDP method is used to visualize the vibration signal, and the two state identification evidence bodies based on the vibration information source are obtained through the VGG16 and ResNet models. Secondly, combined with image monitoring signals, the fatigue defect identification method based on automatic weighting threshold and the detection error dynamic compensation method based on fatigue defect edge features are used to quantify the fatigue damage area and obtain the state identification evidence body based on the image information source. On this basis, a state identification network model based on vibration and image spatiotemporal heterogeneous data is constructed, and the D-S evidence theory is used to realize the contact fatigue state identification of the specimen. The results show that fusion of vibration and image data can achieve information complementarity and may identify the contact fatigue state of the specimen more accurately. The accuracy of state identification after fusion is 98.67%, which is at least 3% higher than that of a single information source. This research is of great significance for the accurate acquisition of material contact fatigue properties and has certain reference value for the heterogeneous data fusion from different sources.
Shear-Bond Behaviour of Profiled Composite Slab Incorporated with Self-Compacted Geopolymer Concrete
Authors: Heweidak Mohamed , Kafle Bidur , Al-Ameri Riyadh
Published: August 2022

Composite slab systems have become increasingly popular over the last few decades because of the advantages of merging the two building materials, profiled steel sheets and concrete. The profiled composite slab’s performance depends on the composite interaction at the longitudinal direction of the concrete–steel interface. Geopolymer concrete has emerged over the last few years as a potential sustainable construction material, with 80% less carbon dioxide emissions than cementitious concrete. Recently, self-compacted geopolymer concrete (SCGC) has been developed, synthesised from a fly ash/slag ratio equal to 60/40, micro fly ash (5%), anhydrous sodium metasilicate solid powder as the alkali-activator and a water/solid content ratio equal to 0.45. The production of SCGC eliminates the need for an elevated temperature during curing and high corrosive alkali-activator solutions, as in traditional geopolymer concrete. The bond characteristics of the profiled composite slab system incorporated with the SCGC mix have not yet been thoroughly investigated. The cost-effectiveness of small-scale tests has popularised its usage by many researchers as an alternative technique to large-scale testing for assessing composite slab load shear capacity. In this paper, small-scale push tests were conducted to investigate the load slip behaviour of the SCGC composite slab compared to the normal concrete (NC) composite slab, with targeted compressive strengths of 40 and 60 MPa. The results indicate that SCGC has better chemical adhesion with profiled steel sheets than NC. Additionally, the profiled composite slab incorporated with SCGC possesses higher ultimate strength and toughness than the normal concrete composite slab.
Establishment and Saliency Verification of a Visual Translation Method for Cultural Elements of High-Speed Railways: A Case Study of the BZ Railway Line
Authors: Bian Wenyan , Li Junjie , Zhao Ruyue , Wu Xijun , Wu Wei
Published: August 2022

The high-speed railway station symbol system, generated from regional culture translations, not only improves transfer efficiency but also reveals the area’s unique urban cultural context. In this study, we used an eye-tracking technique and virtual reality technology to examine the visual cognitive preferences of the existing cultural translation method used by the Beijing–Zhangjiakou high-speed railway. Then, considering the design and layout of the existing station symbol system, we analyzed the visual saliency of different elements such as images, words, and symbols in three types of spaces in the Taizicheng high-speed railway station. The experiment site was located in the physical laboratory of the School of Architecture and Design at Beijing Jiaotong University. A total of 94 students from different majors were selected to participate in the experiment, with 92 datapoints eventually being deemed valid. The experiment data showed the following. First, the overall significance ranking of three scenes in the Taizicheng station was: S1 (81.10%) > S2 (64.57%) > S3 (49.57%). The cognitive correctness rankings of the number positions of the three scenes were: S1: 5 > 2 > 3 > 1 = 4; S2: 4 > 2 > 3 > 1 > 5; S3: 1 > 3 > 2 > 5 > 4. Second, the significance ranking of the transliteration element in S1 was: Images > Words > Sculptures > Patterns > Colors; S2 was: Patterns > Colors > Words > Images > Sculptures; and S3 was: Colors > Images > Words > Patterns > Sculptures. The results underscore the validity of the Beijing–Zhangjiakou cultural translation and offer a reference for station layout and spatial optimization. Finally, they provide new ideas for the design and layout of station symbol systems.
Climate Change Impact on Yield and Water Use of Rice–Wheat Rotation System in the Huang-Huai-Hai Plain, China
Authors: Zhao Yanxi , Xiao Dengpan , Bai Huizi , Liu De Li , Tang Jianzhao , Qi Yongqing , Shen Yanjun
Published: August 2022

Global climate change has had a significant impact on crop production and agricultural water use. Investigating different future climate scenarios and their possible impacts on crop production and water consumption is critical for proposing effective responses to climate change. In this study, based on daily downscaled climate data from 22 Global Climate Models (GCMs) provided by Coupled Model Intercomparison Project Phase 6 (CMIP6), we applied the well-validated Agricultural Production Systems sIMulator (APSIM) to simulate crop phenology, yield, and water use of the rice–wheat rotation at four representative stations (including Hefei and Shouxian stations in Anhui province and Kunshan and Xuzhou stations in Jiangsu province) across the Huang-Huai-Hai Plain, China during the 2041–2070 period (2050s) under four Shared Socioeconomic Pathways (i.e., SSP126, SSP245, SSP370, and SSP585). The results showed a significant increase in annual mean temperature (Temp) and solar radiation (Rad), and annual total precipitation (Prec) at four investigated stations, except Rad under SSP370. Climate change mainly leads to a consistent advance in wheat phenology, but inconsistent trends in rice phenology across four stations. Moreover, the reproductive growth period (RGP) of wheat was prolonged while that of rice was shorted at three of four stations. Both rice and wheat yields were negatively correlated with Temp, but positively correlated with Rad, Prec, and CO2 concentration ([CO2]). However, crop ET was positively correlated with Rad, but negatively correlated with [CO2], as elevated [CO2] decreased stomatal conductance. Moreover, the water use efficiency (WUE) of rice and wheat was negatively correlated with Temp, but positively correlated with [CO2]. Overall, our study indicated that the change in Temp, Rad, Prec, and [CO2] have different impacts on different crops and at different stations. Therefore, in the impact assessment for climate change, it is necessary to explore and analyze different crops in different regions. Additionally, our study helps to improve understanding of the impacts of climate change on crop production and water consumption and provides data support for the sustainable development of agriculture.
Structural Comparison of hMDH2 Complexed with Natural Substrates and Cofactors: The Importance of Phosphate Binding for Active Conformation and Catalysis
Authors: Eo Yumi , Duong Men Thi Hoai , Ahn Hee-Chul
Published: August 2022

Malate dehydrogenase (MDH), which catalyzes a reversible conversion of L-malate to oxaloacetate, plays essential roles in common metabolic processes, such as the tricarboxylic acid cycle, the oxaloacetate–malate shuttle, and the glyoxylate cycle. MDH2 has lately been recognized as a promising anticancer target; however, the structural information for the human homologue with natural ligands is very limited. In this study, various complex structures of hMDH2, with its substrates and/or cofactors, were solved by X-ray crystallography, which could offer knowledge about the molecular and enzymatic mechanism of this enzyme and be utilized to design novel inhibitors. The structural comparison suggests that phosphate binds to the substrate binding site and brings the conformational change of the active loop to a closed state, which can secure the substate and cofactor to facilitate enzymatic activity.
Butyrate Induces Modifications of the CTCF-Binding Landscape in Cattle Cells
Authors: Boschiero Clarissa , Gao Yahui , Baldwin Ransom L. , Ma Li , Li Cong-jun , Liu George E.
Published: August 2022

Butyrate is produced in the rumen from microbial fermentation and is related to several functions, including cell differentiation and proliferation. Butyrate supplementation in calves can accelerate rumen development. DNA-protein interactions, such as the CCCTC-binding factor (CTCF), play essential roles in chromatin organization and gene expression regulation. Although CTCF-binding sites have been identified recently in cattle, a deeper characterization, including differentially CTCF-binding sites (DCBS), is vital for a better understanding of butyrate’s role in the chromatin landscape. This study aimed to identify CTCF-binding regions and DCBS under a butyrate-induced condition using ChIP-seq in bovine cells; 61,915 CTCF peaks were identified in the butyrate and 51,347 in the control. From these regions, 2265 DCBS were obtained for the butyrate vs. control comparison, comprising ~90% of induced sites. Most of the butyrate DCBS were in distal intergenic regions, showing a potential role as insulators. Gene ontology enrichment showed crucial terms for the induced DCBS, mainly related to cellular proliferation, cell adhesion, and growth regulation. Interestingly, the ECM-receptor interaction pathway was observed for the induced DCBS. Motif enrichment analysis further identified transcription factors, including CTCF, BORIS, TGIF2, and ZIC3. When DCBS was integrated with RNA-seq data, putative genes were identified for the repressed DCBS, including GATA4. Our study revealed promising candidate genes in bovine cells by a butyrate-induced condition that might be related to the regulation of rumen development, such as integrins, keratins, and collagens. These results provide a better understanding of the function of butyrate in cattle rumen development and chromatin landscape regulation.
Lipotoxicity as a Barrier for T Cell-Based Therapies
Authors: Böttcher-Loschinski Romy , Rial Saborido Judit , Böttcher Martin , Kahlfuss Sascha , Mougiakakos Dimitrios
Published: August 2022

Nowadays, T-cell-based approaches play an increasing role in cancer treatment. In particular, the use of (genetically engineered) T-cells has heralded a novel era for various diseases with previously poor outcomes. Concurrently, the relationship between the functional behavior of immune cells and their metabolic state, known as immunometabolism, has been found to be an important determinant for the success of immunotherapy. In this context, immune cell metabolism is not only controlled by the expression of transcription factors, enzymes and transport proteins but also by nutrient availability and the presence of intermediate metabolites. The lack of as well as an oversupply of nutrients can be detrimental and lead to cellular dysfunction and damage, potentially resulting in reduced metabolic fitness and/or cell death. This review focusses on the detrimental effects of excessive exposure of T cells to fatty acids, known as lipotoxicity, in the context of an altered lipid tumor microenvironment. Furthermore, implications of T cell-related lipotoxicity for immunotherapy will be discussed, as well as potential therapeutic approaches.
Identification of the Natural Steroid Sapogenin Diosgenin as a Direct Dual-Specific RORα/γ Inverse Agonist
Authors: Schwarz Patrik F. , Perhal Alexander F. , Schöberl Lucia N. , Kraus Martin M. , Kirchmair Johannes , Dirsch Verena M.
Published: August 2022

The steroid sapogenin diosgenin is a well-known natural product with a plethora of described pharmacological activities including the amelioration of T helper 17 (Th17)-driven pathologies. However, the exact underlying mode of action of diosgenin leading to a dampened Th17 response is still largely unknown and specific molecular targets have yet to be identified. Here, we show that diosgenin acts as a direct ligand and inverse agonist of the nuclear receptor retinoic acid receptor (RAR)-related orphan receptor (ROR)α and RORγ, which are key transcription factors involved in Th17 cell differentiation and metabolism. IC50 values determined by luciferase reporter gene assays, employing constructs for either RORγ-Gal4 fusion proteins or full length receptors, were in the low micromolar range at around 2 µM. To highlight the functional consequences of this RORα/γ inverse agonism, we determined gene expression levels of important ROR target genes, i.e., IL-17A and glucose-6-phosphatase, in relevant cellular in vitro models of Jurkat T and HepG2 cells, respectively, by RT-qPCR (reverse transcription quantitative PCR). Thereby, it was shown that diosgenin leads to a dose-dependent decrease in target gene expressions consistent with its potent cellular ROR inverse agonistic activity. Additionally, in silico dockings of diosgenin to the ROR ligand-binding domain were performed to determine the underlying binding mode. Taken together, our results establish diosgenin as a novel, direct and dual-selective RORα/γ inverse agonist. This finding establishes a direct molecular target for diosgenin for the first time, which can further explain reported amendments in Th17-driven diseases by this compound.
The Antifungal and Antibiofilm Activities of Caffeine against Candida albicans on Polymethyl Methacrylate Denture Base Material
Authors: AlEraky Doaa M. , Abuohashish Hatem M. , Gad Mohammed M. , Alshuyukh Muneer H. , Bugshan Amr S. , Almulhim Khalid S. , Mahmoud Maha M.
Published: August 2022

Background: In this study, the effect of pure caffeine was established against Candida albicans (C. albicans) using different microbiological techniques. Methods: Broth microdilution and colony forming units (CFUs) assays were used to detect the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The Live/Dead fluorescent dyes were implemented to determine the yeast viability. Polymethyl methacrylate acrylic resin (PMMA) discs were prepared to evaluate caffeine’s effects against adherent C. albicans using microplate reader, CFUs, and scanning electron microscope (SEM). Results: caffeine’s MIC was detected around 30 mg/mL, while the MFC was considered at 60 mg/mL. In an agar-well diffusion test, the inhibition zones were wider in caffeine groups. The Live/Dead viability test verified caffeine’s antifungal effects. The optical density of the adherent C. albicans on PMMA discs were lower at 620 nm or 410 nm in caffeine groups. CFU count was also reduced by caffeine treatments. SEM revealed the lower adherent C. albicans count in caffeine groups. The effect of caffeine was dose-dependent at which the 60 mg/mL dose demonstrated the most prominent effect. Conclusion: The study reinforced caffeine’s antifungal and antibiofilm properties and suggested it as an additive, or even an alternative, disinfectant solution for fungal biofilms on denture surfaces.
The Microbiome and Metabolomic Profile of the Transplanted Intestine with Long-Term Function
Authors: Girlanda Raffaelle , Liggett Jedson R. , Jayatilake Meth , Kroemer Alexander , Guerra Juan Francisco , Hawksworth Jason Solomon , Radkani Pejman , Matsumoto Cal S. , Zasloff Michael , Fishbein Thomas M.
Published: August 2022

We analyzed the fecal microbiome by deep sequencing of the 16S ribosomal genes and the metabolomic profiles of 43 intestinal transplant recipients to identify biomarkers of graft function. Stool samples were collected from 23 patients with stable graft function five years or longer after transplant, 15 stable recipients one-year post-transplant and four recipients with refractory rejection and graft loss within one-year post-transplant. Lactobacillus and Streptococcus species were predominant in patients with stable graft function both in the short and long term, with a microbiome profile consistent with the general population. Conversely, Enterococcus species were predominant in patients with refractory rejection as compared to the general population, indicating profound dysbiosis in the context of graft dysfunction. Metabolomic analysis demonstrated significant differences between the three groups, with several metabolites in rejecting recipients clustering as a distinct set. Our study suggests that the bacterial microbiome profile of stable intestinal transplants is similar to the general population, supporting further application of this non-invasive approach to identify biomarkers of intestinal graft function.
Veno-Arterial Extracorporeal Membrane Oxygenation (ECMO) Impairs Bradykinin-Induced Relaxation in Neonatal Porcine Coronary Arteries
Authors: Provitera Livia , Amelio Giacomo S. , Tripodi Matteo , Raffaeli Genny , Macchini Francesco , Amodeo Ilaria , Gulden Silvia , Cortesi Valeria , Manzoni Francesca , Cervellini Gaia , Tomaselli Andrea , Zuanetti Gabriele , Lonati Caterina , Battistin Michele , Kamel Shady , Parente Valeria , Pravatà Valentina , Villa Stefania , Villamor Eduardo , Mosca Fabio , Cavallaro Giacomo
Published: August 2022

Extracorporeal membrane oxygenation (ECMO) is a lifesaving support for respiratory and cardiovascular failure. However, ECMO induces a systemic inflammatory response syndrome that can lead to various complications, including endothelial dysfunction in the cerebral circulation. We aimed to investigate whether ECMO-associated endothelial dysfunction also affected coronary circulation. Ten-day-old piglets were randomized to undergo either 8 h of veno-arterial ECMO (n = 5) or no treatment (Control, n = 5). Hearts were harvested and coronary arteries were dissected and mounted as 3 mm rings in organ baths for isometric force measurement. Following precontraction with the thromboxane prostanoid (TP) receptor agonist U46619, concentration–response curves to the endothelium-dependent vasodilator bradykinin (BK) and the nitric oxide (NO) donor (endothelium-independent vasodilator) sodium nitroprusside (SNP) were performed. Relaxation to BK was studied in the absence or presence of the NO synthase inhibitor Nω-nitro-L-arginine methyl ester HCl (L-NAME). U46619-induced contraction and SNP-induced relaxation were similar in control and ECMO coronary arteries. However, BK-induced relaxation was significantly impaired in the ECMO group (30.4 ± 2.2% vs. 59.2 ± 2.1%; p < 0.0001). When L-NAME was present, no differences in BK-mediated relaxation were observed between the control and ECMO groups. Taken together, our data suggest that ECMO exposure impairs endothelium-derived NO-mediated coronary relaxation. However, there is a NO-independent component in BK-induced relaxation that remains unaffected by ECMO. In addition, the smooth muscle cell response to exogenous NO is not altered by ECMO exposure.
Stem Cell-Based Therapeutic Strategies in Diabetic Wound Healing
Authors: Hsieh Meng-Chien Willie , Wang Wei-Ting , Lin Chuang-Yu , Kuo Yur-Ren , Lee Su-Shin , Hou Ming-Feng , Wu Yi-Chia
Published: August 2022

Impaired wound healing and especially the “all-too-common” occurrence of associated diabetic foot ulcers (DFU) are becoming an increasingly urgent and deteriorating healthcare issue, which drastically impact the quality of life and further heighten the risks of infection and amputation in patients with diabetes mellitus. Amongst the multifactorial wound healing determinants, glycemic dysregulation has been identified to be the primary casual factor of poor wound healing. Unfortunately, current therapeutic modalities merely serve as moderate symptomatic relieves but often fail to completely restore the wound site to its pre-injury state and prevent further recurrence. Stem cell-based therapeutics have been employed for its promising potential to address the root of the problem as they not only exhibit the capacity for self-renewal and differentiation towards multiple lineages, but also have been disclosed to participate in mediating variant growth factors and cytokines. Herein we review the current literatures on the therapeutic benefits of using various kinds of stem cells, including embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs), and adipose-derived stem cells (ASCs) in diabetic wound healing by searching on the PubMed® Database for publications. This study shall serve as an overview of the current body of research with particular focus on autologous ASCs and the laboratory expandable iPSCs in hope of shedding more light on this attractive therapy so as to elevate the efficacy of wound healing that is almost always compromised in diabetic patients.