bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Method for Failure Mode and Effect Analysis Based on the Fermatean Fuzzy Set and Bonferroni Mean Operator
Authors: Han Liangsheng , Xia Mingyi , Yu Yang , He Shuai
Published: May 2024

Failure mode and effects analysis (FMEA) helps to identify the weak points in the processing, manufacturing, and assembly of products and plays an important role in improving product reliability. To address the shortcomings of the existing FMEA methods in terms of the uncertainty treatment of information and not considering the weights and correlations between risk factors, we propose a new FMEA method. In this paper, the Fermatean fuzzy Z-number (FFZN) is proposed by fusing the Fermatean fuzzy number and Z-number. Extending it to the Bonferroni mean (BM) operator, the Fermatean fuzzy Z-number-weighted Bonferroni mean (FFZWBM) operator is proposed. A new FMEA method is proposed based on this operator. In order to overcome the factors not considered in the FMEA method, two new risk factors are proposed and added. The ability of experts to express fuzzy information is enhanced by introducing the FFS. The weights and correlations between the influencing factors can be handled by aggregating the evaluation information using the FFZWBM operator. Finally, the proposed method is applied to an arithmetic example and the accuracy of the proposed method is proved by teaming it with other methods.
Investigating the Influence of Non-Uniform Characteristics of Layered Foundation on Ground Vibration Using an Efficient 2.5D Random Finite Element Method
Authors: Yao Shaofeng , Yue Liang , Xie Wei , Zheng Sen , Tang Shuo , Liu Jinglong , Wang Wenkai
Published: May 2024

High-speed train operation may cause vibration near track facilities and propagate far through the ground, affecting people’s lives, work, and normal use of precision instruments in an urban environment. An efficient numerical method is proposed to calculate the non-uniform ground vibration under a moving high-speed railway load. The theory of stochastic variables is used to describe the soil spatial variability of the non-uniform layered elastic ground, and the coupled 2.5D random finite element method (FEM) is proposed to reduce the computational cost without losing accuracy. Vibration propagation and attenuation of the non-uniform layered ground are investigated and the effect of train speed and soil non-homogeneity are analyzed. Results show that (1) at cross speed and high speed, the homogeneity coefficient of the layered ground has the most important influence on the ground vibration amplitude; (2) the upward acceleration is much larger than the downward acceleration at most speeds, and at cross speed and high speed, the acceleration amplitude decreases with the increase in the homogeneity coefficient; (3) as train speed increases from 60 m/s to 130 m/s, the influencing range of the homogeneity coefficient increases to 10 m from 2 m; and (4) the phenomenon of an in increase in local rebound can be observed in the velocity and acceleration attenuation curve at cross speed when the ground soil has a weaker homogeneity.
Hub-and-Spoke Network Optimization with Flow Delay Cost: The Case of Goods Delivery on Urban Logistics Networks in Eastern China
Authors: Wang Bangjun , Shen Guoqiang , Wang Xingshen , Dong Yunwen , Li Ziyu
Published: May 2024

With respect to a traditional point-to-point (P-P) network, a hub-and-spoke (H-S) network not only uses a smaller number of links/paths but also utilizes the scale economy advantage on consolidated flows on hub–hub links and at hubs. However, the inevitable delays through hubs have always been a critical concern. Therefore, this paper develops an H-S model considering flow delay costs and applies the model to a logistics case in Eastern China. The integer quadratic term in the model’s objective function is linearized using the algebraic method. Our model is applied to develop an H-S network for its 13-node express package delivery operation, using the particle swarm optimization (PSO) algorithm. The results show using the H-S can save more than 14.1% of the total cost annually. The model also provides an applied case to the H-S configuration, especially for urban express delivery logistics in China.
Thermostatistics, Information, Subjectivity: Why Is This Association So Disturbing?
Authors: Lairez Didier
Published: May 2024

Although information theory resolves the inconsistencies (known in the form of famous enigmas) of the traditional approach of thermostatistics, its place in the corresponding literature is not what it deserves. This article supports the idea that this is mainly due to epistemological rather than scientific reasons: the subjectivity introduced into physics is perceived as a problem. Here is an attempt to expose and clarify where exactly this subjectivity lies: in the representation of reality and in probabilistic inference, two aspects that have been integrated into the practice of science for a long time and which should no longer frighten anyone but have become explicit with information theory.
Numerical Reconstruction of Time-Dependent Boundary Conditions to 2D Heat Equation on Disjoint Rectangles at Integral Observations
Authors: Koleva Miglena N. , Vulkov Lubin G.
Published: May 2024

In this paper, two-dimensional (2D) heat equations on disjoint rectangles are considered. The solutions are connected by interface Robin’s-type internal conditions. The problem has external Dirichlet boundary conditions that, in the forward (direct) formulation, are given functions. In the inverse problem formulation, the Dirichlet conditions are unknown functions, and the aim is to be reconstructed upon integral observations. Well-posedness both for direct and inverse problems is established. Using the given 2D integrals of the unknown solution on each of the domains and the specific interface boundary conditions, we reduce the 2D inverse problem to a forward heat 1D one. The resulting 1D problem is solved using the explicit Saul’yev finite difference method. Numerical test examples are discussed to illustrate the efficiency of the approach.
The Maximal and Minimal Distributions of Wealth Processes in Black–Scholes Markets
Authors: Liu Shuhui
Published: May 2024

The Black–Scholes formula is an important formula for pricing a contingent claim in complete financial markets. This formula can be obtained under the assumption that the investor’s strategy is carried out according to a self-financing criterion; hence, there arise a set of self-financing portfolios corresponding to different contingent claims. The natural questions are: If an investor invests according to self-financing portfolios in the financial market, what are the maximal and minimal distributions of the investor’s wealth on some specific interval at the terminal time? Furthermore, if such distributions exist, how can the corresponding optimal portfolios be constructed? The present study applies the theory of backward stochastic differential equations in order to obtain an affirmative answer to the above questions. That is, the explicit formulations for the maximal and minimal distributions of wealth when adopting self-financing strategies would be derived, and the corresponding optimal (self-financing) portfolios would be constructed. Furthermore, this would verify the benefits of diversified portfolios in financial markets: that is, do not put all your eggs in the same basket.
A Blow-Up Criterion for the Density-Dependent Incompressible Magnetohydrodynamic System with Zero Viscosity
Authors: Shi Kunlong , Fan Jishan , Nakamura Gen
Published: May 2024

In this paper, we provide a blow-up criterion for the density-dependent incompressible magnetohydrodynamic system with zero viscosity. The proof uses the Lp-method and the Kato–Ponce inequalities in the harmonic analysis. The novelty of our work lies in the fact that we deal with the case in which the resistivity η is positive.
Enhancing Security and Efficiency: A Fine-Grained Searchable Scheme for Encryption of Big Data in Cloud-Based Smart Grids
Authors: Wen Jing , Li Haifeng , Liu Liangliang , Lan Caihui
Published: May 2024

The smart grid, as a crucial part of modern energy systems, handles extensive and diverse data, including inputs from various sensors, metering devices, and user interactions. Outsourcing data storage to remote cloud servers presents an economical solution for enhancing data management within the smart grid ecosystem. However, ensuring data privacy before transmitting it to the cloud is a critical consideration. Therefore, it is common practice to encrypt the data before uploading them to the cloud. While encryption provides data confidentiality, it may also introduce potential issues such as limiting data owners’ ability to query their data. The searchable attribute-based encryption (SABE) not only enables fine-grained access control in a dynamic large-scale environment but also allows for data searches on the ciphertext domain, making it an effective tool for cloud data sharing. Although SABE has become a research hotspot, existing schemes often have limitations in terms of computing efficiency on the client side, weak security of the ciphertext and the trapdoor. To address these issues, we propose an efficient server-aided ciphertext-policy searchable attribute-based encryption scheme (SA-CP-SABE). In SA-CP-SABE, the user’s data access authority is consistent with the search authority. During the search process, calculations are performed not only to determine whether the ciphertext matches the keyword in the trapdoor, but also to assist subsequent user ciphertext decryption by reducing computational complexity. Our scheme has been proven under the random oracle model to achieve the indistinguishability of the ciphertext and the trapdoor and to resist keyword-guessing attacks. Finally, the performance analysis and simulation of the proposed scheme are provided, and the results show that it performs with high efficiency.
Structural Shape Optimization Based on Multi-Patch Weakly Singular IGABEM and Particle Swarm Optimization Algorithm in Two-Dimensional Elastostatics
Authors: Chen Zhenyu , Xie Longtao
Published: May 2024

In this paper, a multi-patch weakly singular isogeometric boundary element method (WSIGABEM) for two-dimensional elastostatics is proposed. Since the method is based on the weakly singular boundary integral equation, quadrature techniques, dedicated to the weakly singular and regular integrals, are applied in the method. A new formula for the generation of collocation points is suggested to take full advantage of the multi-patch technique. The generated collocation points are essentially inside the patches without any correction. If the boundary conditions are assumed to be continuous in every patch, no collocation point lies on the discontinuous boundaries, thus simplifying the implementation. The multi-patch WSIGABEM is verified by simple examples with analytical solutions. The features of the present multi-patch WSIGABEM are investigated by comparison with the traditional IGABEM. Furthermore, the combination of the present multi-patch WSIGABEM and the particle swarm optimization algorithm results in a shape optimization method in two-dimensional elastostatics. By changing some specific control points and their weights, the shape optimizations of the fillet corner, the spanner, and the arch bridge are verified to be effective.
Computational Insights into Cyclodextrin Inclusion Complexes with the Organophosphorus Flame Retardant DOPO
Authors: Ma Le , Zhang Yongguang , Zhang Puyu , Zhang Haiyang
Published: May 2024

Cyclodextrins (CDs) were used as green char promoters in the formulation of organophosphorus flame retardants (OPFRs) for polymeric materials, and they could reduce the amount of usage of OPFRs and their release into the environment by forming [host:guest] inclusion complexes with them. Here, we report a systematic study on the inclusion complexes of natural CDs (α-, β-, and γ-CD) with a representative OPFR of DOPO using computational methods of molecular docking, molecular dynamics (MD) simulations, and quantum mechanical (QM) calculations. The binding modes and energetics of [host:guest] inclusion complexes were analyzed in details. α-CD was not able to form a complete inclusion complex with DOPO, and the center of mass distance [host:guest] distance amounted to 4–5 Å. β-CD and γ-CD allowed for a deep insertion of DOPO into their hydrophobic cavities, and DOPO was able to frequently change its orientation within the γ-CD cavity. The energy decomposition analysis based on the dispersion-corrected density functional theory (sobEDAw) indicated that electrostatic, orbital, and dispersion contributions favored [host:guest] complexation, while the exchange–repulsion term showed the opposite. This work provides an in-depth understanding of using CD inclusion complexes in OPFRs formulations.
Integrated Metabolomics and Transcriptomics Analysis of Flavonoid Biosynthesis Pathway in Polygonatum cyrtonema Hua
Authors: Yang Luyun , Yang Qingwen , Zhang Luping , Ren Fengxiao , Zhang Zhouyao , Jia Qiaojun
Published: May 2024

Flavonoids, a class of phenolic compounds, are one of the main functional components and have a wide range of molecular structures and biological activities in Polygonatum. A few of them, including homoisoflavonoids, chalcones, isoflavones, and flavones, were identified in Polygonatum and displayed a wide range of powerful biological activities, such as anti-cancer, anti-viral, and blood sugar regulation. However, few studies have systematically been published on the flavonoid biosynthesis pathway in Polygonatum cyrtonema Hua. Therefore, in the present study, a combined transcriptome and metabolome analysis was performed on the leaf, stem, rhizome, and root tissues of P. cyrtonema to uncover the synthesis pathway of flavonoids and to identify key regulatory genes. Flavonoid-targeted metabolomics detected a total of 65 active substances from four different tissues, among which 49 substances were first study to identify in Polygonatum, and 38 substances were flavonoids. A total of 19 differentially accumulated metabolites (DAMs) (five flavonols, three flavones, two dihydrochalcones, two flavanones, one flavanol, five phenylpropanoids, and one coumarin) were finally screened by KEGG enrichment analysis. Transcriptome analysis indicated that a total of 222 unigenes encoding 28 enzymes were annotated into three flavonoid biosynthesis pathways, which were “phenylpropanoid biosynthesis”, “flavonoid biosynthesis”, and “flavone and flavonol biosynthesis”. The combined analysis of the metabolome and transcriptome revealed that 37 differentially expressed genes (DEGs) encoding 11 enzymes (C4H, PAL, 4CL, CHS, CHI, F3H, DFR, LAR, ANR, FNS, FLS) and 19 DAMs were more likely to be regulated in the flavonoid biosynthesis pathway. The expression of 11 DEGs was validated by qRT-PCR, resulting in good agreement with the RNA-Seq. Our studies provide a theoretical basis for further elucidating the flavonoid biosynthesis pathway in Polygonatum.
Effect of the Addition of Dandelion (Taraxacum officinale) on the Protein Profile, Antiradical Activity, and Microbiological Status of Raw-Ripening Pork Sausage
Authors: Wójciak Karolina , Materska Małgorzata , Pełka Arkadiusz , Michalska Agata , Małecka-Massalska Teresa , Kačániová Miroslava , Čmiková Natália , Słowiński Mirosław
Published: May 2024

The study evaluated the effect of adding dandelion extract on the characteristics of raw-ripening pork sausages while reducing the nitrite addition from 150 to 80 mg/kg. The sausages were made primarily from pork ham (80%) and pork jowl (20%). The process involved curing, preparing the meat stuffing, forming the links, and then subjecting the sausages to a 21-day ripening period. Physicochemical parameters such as pH, water activity, and oxidation-reduction potential were compared at the beginning of production and after the ripening process. The study also examined the impact of ripening on protein metabolism in pork sausages and compared the protein profiles of different sausage variants. The obtained research results indicate that dandelion-leaf extract (Taraxacum officinale) were rich in phenolic acids, flavonoids, coumarins, and their derivatives (LC-QTOF-MS method). Antiradical activity test against the ABTS+* and DPPH radical, and the TBARS index, demonstrated that addition of dandelion (0.5–1%) significantly improved the oxidative stability of raw-ripening sausages with nitrite content reduction to 80 mg/kg. A microbiological evaluation of the sausages was also carried out to assess the correctness of the ripening process. The total number of viable bacteria, lactic acid bacteria, and coliforms were evaluated and subsequently identified by mass spectrometry.
Correction: Liu et al. Rational Design of Nitrogen-Doped Carbon Dots for Inhibiting β-Amyloid Aggregation. Molecules 2023, 28, 1451
Authors: Liu Hong , Guo Huazhang , Fang Yibin , Wang Liang , Li Peng
Published: May 2024

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Quantum Magnetism of the Iron Core in Ferritin Proteins—A Re-Evaluation of the Giant-Spin Model
Authors: Hagen Wilfred R.
Published: May 2024

The electron–electron, or zero-field interaction (ZFI) in the electron paramagnetic resonance (EPR) of high-spin transition ions in metalloproteins and coordination complexes, is commonly described by a simple spin Hamiltonian that is second-order in the spin S: H=D[Sz2−SS+1/3+E(Sx2−Sy2). Symmetry considerations, however, allow for fourth-order terms when S ≥ 2. In metalloprotein EPR studies, these terms have rarely been explored. Metal ions can cluster via non-metal bridges, as, for example, in iron-sulfur clusters, in which exchange interaction can result in higher system spin, and this would allow for sixth- and higher-order ZFI terms. For metalloproteins, these have thus far been completely ignored. Single-molecule magnets (SMMs) are multi-metal ion high spin complexes, in which the ZFI usually has a negative sign, thus affording a ground state level pair with maximal spin quantum number mS = ±S, giving rise to unusual magnetic properties at low temperatures. The description of EPR from SMMs is commonly cast in terms of the ‘giant-spin model’, which assumes a magnetically isolated system spin, and in which fourth-order, and recently, even sixth-order ZFI terms have been found to be required. A special version of the giant-spin model, adopted for scaling-up to system spins of order S ≈ 103–104, has been applied to the ubiquitous iron-storage protein ferritin, which has an internal core containing Fe3+ ions whose individual high spins couple in a way to create a superparamagnet at ambient temperature with very high system spin reminiscent to that of ferromagnetic nanoparticles. This scaled giant-spin model is critically evaluated; limitations and future possibilities are explicitly formulated.
Comparison of Various Theoretical Measures of Aromaticity within Monosubstituted Benzene
Authors: Swain Caleb K. , Scheiner Steve
Published: May 2024

The effects of monosubstitution on the aromaticity of benzene are assessed using a number of different quantitative schemes. The ability of the mobile π-electrons to respond to an external magnetic field is evaluated using several variants of the NICS scheme which calculate the shielding of points along the axis perpendicular to the molecule. Another class of measures is related to the drive toward the uniformity of C-C bond lengths and strengths. Several energetic quantities are devised to approximate an aromatic stabilization energy and the tendency of the molecule to maintain planarity. There is a lack of consistency in that the various measures of aromaticity lead to differing conclusions as to the effects of substituents on the aromaticity of the ring.
Advancements in Nanosystems for Ocular Drug Delivery: A Focus on Pediatric Retinoblastoma
Authors: Wu Kevin Y. , Wang Xingao C. , Anderson Maude , Tran Simon D.
Published: May 2024

The eye’s complex anatomical structures present formidable barriers to effective drug delivery across a range of ocular diseases, from anterior to posterior segment pathologies. Emerging as a promising solution to these challenges, nanotechnology-based platforms—including but not limited to liposomes, dendrimers, and micelles—have shown the potential to revolutionize ophthalmic therapeutics. These nanocarriers enhance drug bioavailability, increase residence time in targeted ocular tissues, and offer precise, localized delivery, minimizing systemic side effects. Focusing on pediatric ophthalmology, particularly on retinoblastoma, this review delves into the recent advancements in functionalized nanosystems for drug delivery. Covering the literature from 2017 to 2023, it comprehensively examines these nanocarriers’ potential impact on transforming the treatment landscape for retinoblastoma. The review highlights the critical role of these platforms in overcoming the unique pediatric eye barriers, thus enhancing treatment efficacy. It underscores the necessity for ongoing research to realize the full clinical potential of these innovative drug delivery systems in pediatric ophthalmology.
Recent Advances in Manganese(III)-Assisted Radical Cyclization for the Synthesis of Natural Products: A Comprehensive Review
Authors: Biçer Emre , Yılmaz Mehmet
Published: May 2024

Natural products play an important part in synthetic chemistry since they have many pharmacological properties and are used as active drug compounds in pharmaceutical chemistry. However, synthesis of these complex molecules is difficult due to the requirement of various synthetic steps, which include highly regio- and stereoselectivity. Therefore, oxidative radical cyclization assisted by manganese(III) acetate serves as an important step in obtaining spiro-, tricyclic, tetracyclic, and polycyclic derivatives of these compounds. Manganese(III)-based reactions offer a single-step regio- and stereoselective cyclizations and α-acetoxidations, reducing the number of synthetic steps. Also, the manganese(III)-mediated oxidative free radical cyclization method has been successfully applied for the synthesis of cyclic structures found in many natural products. This article presents a broad overview of manganese(III)-based radical reactions of natural products as a key step in overall synthesis. The authors have classified natural product synthesis processes assisted by manganese(III) acetate as intermolecular, intramolecular, oxidation, acetoxidation, aromatization, and polymerization reactions, respectively.
Chemical and Bioactive Properties of Red Rice with Potential Pharmaceutical Use
Authors: Baptista Eugénia , Liberal Ângela , Cardoso Rossana V. C. , Fernandes Ângela , Dias Maria Inês , Pires Tânia C.S.P. , Calhelha Ricardo C. , García Pablo A. , Ferreira Isabel C.F.R. , Barreira João C.M.
Published: May 2024

Red rice has been proposed as a super-food. Accordingly, the nutritional properties (AOAC), as well as its chemical composition, including sugars (HPLC-RI), organic acids (UFLC-PDA), tocopherols (HPLD-FD), and phenolic compounds (LC-DAD-ESI/MSn), together with the main bioactive properties (antioxidant, cytotoxic, antiproliferative, and antibacterial activities), were evaluated to access its nutritional benefits and health improvement potential. The most abundant macronutrients found were carbohydrates (87.2 g/100 g dw), proceeded by proteins (9.1 g/100 g dw), fat (2.6 g/100 g dw), and ash (1.1 g/100 g dw). Sucrose and raffinose were the only detected sugars, with sucrose presenting the maximum concentration (0.74 g/100 g dw). MUFAs and PUFAs were the predominant fatty acids (40.7% and 31%, respectively). Among the two detected tocopherol isoforms, γ-tocopherol (0.67 mg/100 g dw) predominated over α-tocopherol. The phenolic compounds profile, majorly composed of flavan-3-ols, should be associated with the detected bioactivities, which may provide biological benefits to human health beyond the primary nutritional effect. Overall, the bioactive potential of red rice was comprehensively accessed.
A Review of the Phytochemistry and Biological Activities of Echinopsis Radix
Authors: Sun Na , Wang Yujing , Ma Shuo , Kang Huanhuan , Zhou Caihong , Jin Linxuan , Zhang Xin , Zhang Yu , Yuan Yuhan , Shu Penghua
Published: May 2024

The main varieties of Echinopsis Radix recorded in the Chinese Pharmacopoeia are the roots of Echinops latifolius Tausch or Echinops grijsii Hance. However, the chemical constituents and biological activities of this herb have not been reviewed. In order to clarify the chemical constituents of the main varieties of this herb and improve the quality of Chinese medicinal material resources, this paper systematically reviewed their chemical constituents and related biological activities. Phytochemical investigations reveal eighty-five compounds including fort y-nine thiophenes (1–49), eight flavonoids (50–57), seven caffeic acids and its derivatives (58–64), eight sesquiterpenoids (65–72), and thirteen triterpenoids and other compounds (73–85) were reported from Echinopsis Radix. The review of biological activities suggests that thiophenes are the main secondary metabolites of the medicinal material which exert antitumor, insecticidal and antifungal activities. In addition, caffeic acid and its derivatives and sesquiterpenes are potential active ingredients worthy of further study. This review provides an important scientific basis for the development of active ingredients and resource quality evaluation of Echinopsis Radix.
Recognition of 8-Oxo-2′-deoxyguanosine in DNA Using the Triphosphate of 2′-Deoxycytidine Connecting the 1,3-Diazaphenoxazine Unit, dCdapTP
Authors: Sakurada Takato , Chikada Yuta , Miyahara Ryo , Taniguchi Yosuke
Published: May 2024

DNA is constantly damaged by various external and internal factors. In particular, oxidative damage occurs in a steady state, and 8-oxo-2′-deoxyguanosine (oxodG) is known as the main oxidative damage. OxodG is a strong genotoxic nucleoside and is thought to be involved in the pathogenesis of cancer and neurological diseases. However, a breakthrough method to detect the position of oxodG in DNA has not yet been developed. Therefore, we attempted to develop a novel method to detect oxodG in DNA using artificial nucleosides. Recently, we have succeeded in the recognition of oxodG in DNA by a single nucleotide elongation reaction using nucleoside derivatives based on a purine skeleton with a 1,3-diazaphenoxazine unit. In this study, we developed a new nucleoside derivative with a pyrimidine skeleton in order to further improve the recognition ability and enzymatic reaction efficiency. We, therefore, designed and synthesized 2′-deoxycytidine-1,3-diazaphenoxazine (Cdap) and its triphosphate derivatives. The results showed that it was incorporated into the primer strand relative to the dG template because of its cytidine skeleton, but it was more effective at the complementary position of the oxodG template. These results indicate that the new nucleoside derivative can be considered as one of the new candidates for the detection of oxodG in DNA.
One-Pot Cu/SAPO-34 for Continuous Methane Selective Oxidation to Methanol
Authors: Sun Lanlan , Wang Yu , Gu Xuesong , Zhao Meng , Yuan Lijuan
Published: May 2024

Cu/SAPO-34 synthesized via a one-pot method with relatively low silicon content and copper loading at around 2 wt.% facilitated continuous oxidation of methane to methanol with a methanol space time yield of 504 μmolCH3OH/gcat/h. Remarkably, the methanol yield exceeded 1800 mmolCH3OH/molCu/h at 623 K. Typically, the presence of trace oxygen in the system was the key to maintaining the high selectivity to methanol. Characterization results from a series of techniques, including XRD, SEM, TEM, H2-TPR, NH3-TPD, UV-vis, and FTIR, indicated that Cu2+ existed in the position where it moves from hexagonal rings to elliptical cages as the active center.
Impact of Condition Variations on Bioelectrochemical System Performance: An Experimental Investigation of Sulfamethoxazole Degradation
Authors: Xue Qun , Chen Zhihui , Xie Wenjing , Zhang Shuke , Jiang Jie , Sun Guoxin
Published: May 2024

Bioelectrochemical systems (BESs) are an innovative technology for the efficient degradation of antibiotics. Shewanella oneidensis (S. oneidensis) MR-1 plays a pivotal role in degrading sulfamethoxazole (SMX) in BESs. Our study investigated the effect of BES conditions on SMX degradation, focusing on microbial activity. The results revealed that BESs operating with a 0.05 M electrolyte concentration and 2 mA/cm2 current density outperformed electrolysis cells (ECs). Additionally, higher electrolyte concentrations and elevated current density reduced SMX degradation efficiency. The presence of nutrients had minimal effect on the growth of S. oneidensis MR-1 in BESs; it indicates that S. oneidensis MR-1 can degrade SMX without nutrients in a short period of time. We also highlighted the significance of mass transfer between the cathode and anode. Limiting mass transfer at a 10 cm electrode distance enhanced S. oneidensis MR-1 activity and BES performance. In summary, this study reveals the complex interaction of factors affecting the efficiency of BES degradation of antibiotics and provides support for environmental pollution control.
Carvacrol and Thymol Hybrids: Potential Anticancer and Antibacterial Therapeutics
Authors: Peter Sijongesonke , Sotondoshe Namhla , Aderibigbe Blessing A.
Published: May 2024

Cancer is ranked among lethal diseases globally, and the increasing number of cancer cases and deaths results from limited access to effective therapeutics. The use of plant-based medicine has been gaining interest from several researchers. Carvacrol and its isomeric compound, thymol, are plant-based extracts that possess several biological activities, such as antimalarial, anticancer, antifungal, and antibacterial. However, their efficacy is compromised by their poor bioavailability. Thus, medicinal scientists have explored the synthesis of hybrid compounds containing their pharmacophores to enhance their therapeutic efficacy and improve their bioavailability. Hence, this review is a comprehensive report on hybrid compounds containing carvacrol and its isomer, thymol, with potent anticancer and antibacterial agents reported between 2020 and 2024. Furthermore, their structural activity relationship (SAR) and recommended future strategies to further enhance their therapeutic effects will be discussed.
Innovative Solid-Phase Extraction Strategies for Improving the Advanced Chromatographic Determination of Drugs in Challenging Biological Samples
Authors: Mahdavijalal Mohammadreza , Petio Carmine , Staffilano Giovanni , Mandrioli Roberto , Protti Michele
Published: May 2024

In the past few decades, considerable scientific strides have been made in the subject of drug analysis in human biological samples. However, the risk caused by incorrect drug plasma levels in patients still remains an important concern. This review paper attempts to investigate the advances made over the last ten years in common sample preparation techniques (SPT) for biological samples based on solid sorbents, including solid-phase extraction (SPE) and solid-phase micro-extraction (SPME), and in particular in the field of molecularly imprinted polymers (MIPs), including non-stimuli-responsive and stimuli-responsive adsorbents. This class of materials is known as ‘smart adsorbents’, exhibiting tailored responses to various stimuli such as magnetic fields, pH, temperature, and light. Details are provided on how these advanced SPT are changing the landscape of modern drug analysis in their coupling with liquid chromatography-mass spectrometry (LC-MS) analytical techniques, a general term that includes high-performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC), as well as any variation of MS, such as tandem (MS/MS), multiple-stage (MSn), and high-resolution (HRMS) mass spectrometry. Some notes are also provided on coupling with less-performing techniques, such as high-performance liquid chromatography with ultraviolet (HPLC-UV) and diode array detection (HPLC-DAD) detection. Finally, we provide a general review of the difficulties and benefits of the proposed approaches and the future prospects of this research area.
Unraveling the Impact of Adsorbed Molecules on Photocatalytic Processes: Advancements in Understanding Facet-Controlled Semiconductor Photocatalysts
Authors: Kusior Anna , Michalec Kinga , Micek-Ilnicka Anna , Radecka Marta
Published: May 2024

This work aims to demonstrate that the Fe2O3 nanocrystals’ adsorptive and photocatalytic properties can be adjusted by exposing the crystal facets that are functionalized. To this end, cube- and disc-like structures were synthesized using a metal ion-mediated hydrothermal route. Thereafter, some of the samples were annealed at 500 °C for 3 h. Our paper combines the experimental part with theoretical calculations of the obtained materials’ band edge positions. The results reveal that—aside from hematite—the as-synthesized discs also contain γ-FeOOH and β-Fe2O3 phases, which transform into α-Fe2O3 during annealing. The hydrodynamic diameter, zeta potential, and adsorption kinetics measurements show that the cube-like samples exhibit the highest affinity for cationic, whereas the discs have an affinity for anionic dye. Measurements of the wall zeta potential also reveal that annealing the discs modifies their surface state and ability to adsorb molecules. Photocatalytic tests show that the as-synthesized powders have better photocatalytic performance toward methylene blue decomposition than the annealed ones. The observed small changes in the concentration of the MO during illumination result from the energy band structure of the cube-like crystal orientation.
Research Progress on Taxus Extraction and Formulation Preparation Technologies
Authors: Gao Xinyu , Chen Kuilin , Xie Weidong
Published: May 2024

Taxus, as a globally prevalent evergreen tree, contains a wealth of bioactive components that play a crucial role in the pharmaceutical field. Taxus extracts, defined as a collection of one or more bioactive compounds extracted from the genus Taxus spp., have become a significant focus of modern cancer treatment research. This review article aims to delve into the scientific background of Taxus extracts and their considerable value in pharmaceutical research. It meticulously sifts through and compares various advanced extraction techniques such as supercritical extraction, ultrasound extraction, microwave-assisted extraction, solid-phase extraction, high-pressure pulsed electric field extraction, and enzymatic extraction, assessing each technology’s advantages and limitations across dimensions such as extraction efficiency, extraction purity, economic cost, operational time, and environmental impact, with comprehensive analysis results presented in table form. In the area of drug formulation design, this paper systematically discusses the development strategies for solid, liquid, and semi-solid dosage forms based on the unique physicochemical properties of Taxus extracts, their intended medical uses, and specific release characteristics, delving deeply into the selection of excipients and the critical technical issues in the drug preparation process. Moreover, the article looks forward to the potential directions of Taxus extracts in future research and medical applications, emphasizing the urgency and importance of continuously optimizing extraction methods and formulation design to enhance treatment efficacy, reduce production costs, and decrease environmental burdens. It provides a comprehensive set of preparation techniques and formulation optimization schemes for researchers in cancer treatment and other medical fields, promoting the application and development of Taxus extracts in pharmaceutical sciences.
Do Young Consumers Care about Antioxidant Benefits and Resveratrol and Caffeic Acid Consumption?
Authors: Gălbău Cristina Ștefania , Badea Mihaela , Gaman Laura Elena
Published: May 2024

Resveratrol and caffeic acid are some of the most consumed antioxidants during the day, so their importance as sources and their benefits need to be evaluated and updated. This survey aimed not only to analyze whether young Romanian consumers are informed about the benefits of antioxidants in general, and resveratrol and caffeic acid in particular, but also to observe the degree of nutritional education of these participants. Young consumers know the concept of antioxidants relatively well; they managed to give examples of antioxidants and indicate their effects. The majority of those chosen drink wine and coffee, but many are unaware of their health advantages and antioxidant properties. Students are less familiar with the antioxidant chemicals resveratrol and caffeic acid. It is advised to have a thorough understanding of these significant antioxidants and their nutritional content as they are present in our regular diets, and further studies on different kinds of antioxidants are required to increase the awareness of people concerning their importance in daily life.
Adding Branched-Chain Amino Acids and Vitamin D to Whey Protein Is More Effective than Protein Alone in Preserving Fat Free Mass and Muscle Strength in the First Month after Sleeve Gastrectomy
Authors: Schiavo Luigi , Santella Biagio , Paolini Barbara , Rahimi Farnaz , Giglio Emmanuele , Martinelli Barbara , Boschetti Stefano , Bertolani Lilia , Gennai Katia , Arolfo Simone , Bertani Maria Paola , Pilone Vincenzo
Published: May 2024

Objectives: Sleeve gastrectomy (SG) is one of the most commonly performed weight loss (WL) bariatric procedures. The main goal of WL is reducing total body weight (TBW) and fat mass (FM). However, TBW loss is systematically accompanied by a decline in fat-free mass (FFM), predominantly in the first post-surgical month, despite protein supplementation. Branched-chain amino acids (BCAAs) and vitamin D seem to attenuate loss of FFM and, thus, reduce the decline in muscle strength (MS). However, data on the role of an integrated supplementation with whey protein plus BCAAs plus vitamin D (P+BCAAs+Vit.D) vs. protein alone on total weight loss (TWL), fat mass (FM), fat-free mass (FFM), and (MS) in the first month after SG are lacking. Therefore, the present study aims to evaluate the impact of P+BCAAs+Vit.D vs. protein alone supplementation on TWL, FM, FFM, and MS in the first month after SG. Materials and Methods: Before SG and at 1 month afterward, we prospectively measured and compared TBW, FM, FFM, and MS in 57 patients who received either a supplementation with P+BCAAs+Vit.D (n = 31) or protein alone (n = 26). The impact of P+BCAAs+Vit.D and protein alone supplementation on clinical status was also evaluated. Results: Despite non-significant variation in TBW, FM decreased more significantly (18.5% vs. 13.2%, p = 0.023) with the P+BCAA+Vit.D supplementation compared to protein alone. Furthermore, the P+BCAA+Vit.D group showed a significantly lower decrease in FFM (4.1% vs. 11.4%, p < 0.001) and MS (3.8% vs. 18.5%, p < 0.001) compared to the protein alone group. No significant alterations in clinical status were seen in either group. Conclusion: P+BCAA+Vit.D supplementation is more effective than protein alone in determining FM loss and is associated with a lower decrease in FFM and MS, without interfering with clinical status in patients 1 month after SG.
The Effect of Hyperbaric Storage on the Nutritional Value and Retention of Certain Bioactive Proteins in Human Milk
Authors: Mazur Katarzyna , Kusznierewicz Barbara , Martysiak-Żurowska Dorota , Drążkowska Izabela , Malinowska-Pańczyk Edyta
Published: May 2024

Human milk (HM) contains the essential macronutrients and bioactive compounds necessary for the normal growth and development of newborns. The milk collected by human milk banks is stored frozen and pasteurized, reducing its nutritional and biological value. The purpose of this study was to determine the effect of hyperbaric storage at subzero temperatures (HS-ST) on the macronutrients and bioactive proteins in HM. As control samples, HM was stored at the same temperatures under 0.1 MPa. A Miris HM analyzer was used to determine the macronutrients and the energy value. The lactoferrin (LF), lysozyme (LYZ) and α-lactalbumin (α-LAC) content was checked using high-performance liquid chromatography, and an ELISA test was used to quantify secretory immunoglobulin A (sIgA). The results showed that the macronutrient content did not change significantly after 90 days of storage at 60 MPa/−5 °C, 78 MPa/−7 °C, 111 MPa/−10 °C or 130 MPa/−12 °C. Retention higher than 90% of LYZ, α-LAC, LF and sIgA was observed in the HM stored at conditions of up to 111 MPa/−10 °C. However, at 130 MPa/−12 °C, there was a reduction in LYZ and LF, by 39 and 89%, respectively. The storage of HM at subzero temperatures at 0.1 MPa did not affect the content of carbohydrates or crude and true protein. For fat and the energy value, significant decreases were observed at −5 °C after 90 days of storage.
The Role of Nutrition and Body Composition on Metabolism
Authors: Wang Yuyang , Chen Botian , Ma Defu
Published: May 2024

Metabolism encompasses the entire array of chemical reactions continuously occurring within the body that sustain life and maintain normal physiological functions [...]
Exploring the Association between Anxiety, Depression, and Gut Microbiota during Pregnancy: Findings from a Pregnancy Cohort Study in Shijiazhuang, Hebei Province, China
Authors: Chi Ruixin , Li Muxia , Zhang Man , Zhang Na , Zhang Guohua , Cui Lijun , Ma Guansheng
Published: May 2024

Negative emotions and gut microbiota during pregnancy both bear significant public health implications. However, the relationship between them has not been fully elucidated. This study, utilizing data from a pregnancy cohort, employed metagenomic sequencing to elucidate the relationship between anxiety, depression, and gut microbiota’s diversity, composition, species, and functional pathways. Data from 87 subjects, spanning 225 time points across early, mid, and late pregnancy, were analyzed. The results revealed that anxiety and depression significantly corresponded to lower alpha diversity (including the Shannon entropy and the Simpson index). Anxiety and depression scores, along with categorical distinctions of anxiety/non-anxiety and depression/non-depression, were found to account for 0.723%, 0.731%, 0.651%, and 0.810% of the variance in gut-microbiota composition (p = 0.001), respectively. Increased anxiety was significantly positively associated with the abundance of Oscillibacter sp. KLE 1745, Oscillibacter sp. PEA192, Oscillibacter sp. KLE 1728, Oscillospiraceae bacterium VE202 24, and Treponema socranskii. A similar association was significantly noted for Oscillibacter sp. KLE 1745 with elevated depression scores. While EC.3.5.3.1: arginase appeared to be higher in the anxious group than in the non-anxious group, vitamin B12-related enzymes appeared to be lower in the depression group than in the non-depression group. The changes were found to be not statistically significant after post-multiple comparison adjustment.
The Next-Generation Probiotic E. coli 1917-pSK18a-MT Ameliorates Cadmium-Induced Liver Injury by Surface Display of Metallothionein and Modulation of Gut Microbiota
Authors: Zhang Yan , Huang Hong , Luo Chuanlin , Zhang Xinfeng , Chen Yanjing , Yue Fenfang , Xie Bingqing , Chen Tingtao , Zou Changwei
Published: May 2024

Cadmium (Cd) is recognized as being linked to several liver diseases. Currently, due to the limited spectrum of drugs available for the treatment of Cd intoxication, developing and designing antidotes with superior detoxification capacity and revealing their underlying mechanisms remains a major challenge. Therefore, we developed the first next-generation probiotic E. coli 1917-pSK18a-MT that delivers metallothionein (MT) to overcome Cd-induced liver injury in C57BL/6 mice by utilizing bacterial surface display technology. The results demonstrate that E. coli 1917-pSK18a-MT could efficiently express MT without altering the growth and probiotic properties of the strain. Moreover, we found that E. coli 1917-pSK18a-MT ameliorated Cd contamination-induced hepatic steatosis, inflammatory cell infiltration, and liver fibrosis by decreasing the expression of aminotransferases along with inflammatory factors. Activation of the Nrf2-Keap1 signaling pathway also further illustrated the hepatoprotective effects of the engineered bacteria. Finally, we showed that E. coli 1917-pSK18a-MT improved the colonic barrier function impaired by Cd induction and ameliorated intestinal flora dysbiosis in Cd-poisoned mice by increasing the relative abundance of the Verrucomicrobiota. These data revealed that the combination of E. coli 1917 and MT both alleviated Cd-induced liver injury to a greater extent and restored the integrity of colonic epithelial tissues and bacterial dysbiosis.
Identifying the Quantitative Trait Locus and Candidate Genes of Traits Related to Milling Quality in Rice via a Genome-Wide Association Study
Authors: Hu Changmin , Li Xinru , Zhang Mengyuan , Jing Chunyu , Hai Mei , Shen Jiaming , Xu Qing , Dang Xiaojing , Shi Yingyao , Liu Erbao , Jiang Jianhua
Published: May 2024

Milling quality directly affects production efficiency in rice, which is closely related to the brown rice recovery (BRR), the milled rice recovery (MRR) and the head milled rice recovery (HMRR). The present study investigated these three traits in 173 germplasms in two environments, finding abundant phenotypic variation. Three QTLs for BRR, two for MRR, and three for HMRR were identified in a genome-wide association study, five of these were identified in previously reported QTLs and three were newly identified. By combining the linkage disequilibrium (LD) analyses, the candidate gene LOC_Os05g08350 was identified. It had two haplotypes with significant differences and Hap 2 increased the BRR by 4.40%. The results of the qRT-PCR showed that the expression of LOC_Os05g08350 in small-BRR accessions was significantly higher than that in large-BRR accessions at Stages 4–5 of young panicle development, reaching the maximum value at Stage 5. The increase in thickness of the spikelet hulls of the accession carrying LOC_Os05g08350TT occurred due to an increase in the cell width and the cell numbers in cross-sections of spikelet hulls. These results help to further clarify the molecular genetic mechanism of milling-quality-related traits and provide genetic germplasm materials for high-quality breeding in rice.
Green Valorization of Waste Plastics to Graphene as an Upcycled Eco-Friendly Material for Advanced Gas Sensing
Authors: Amoh Prince Oppong , Elkady Marwa , Nasr Mahmoud , Shokry Hassan
Published: May 2024

The valorization technique successfully transformed waste polyethylene terephthalate (PET) into valuable carbon nanomaterial (CN)/graphene, while doped and undoped ZnO nanopowders were synthesized via sol–gel methods. Utilizing XRD, BET, TEM, EDX, FTIR, and TGA analyses, the synthesis of sp2 2D sheet, pristine, and doped ZnO nanostructures was confirmed. Solid-state gas sensor devices, tested under 51% relative humidity (RH), 30 °C ambient temperature, and 0.2 flow rate, exhibited a 3.4% enhanced response to H2 gas compared to CO2 at 50 ppm concentrations over time. Notably, the ZnO/CN sensor surpassed CN and ZnO alone, attributed to CN dopant integration with decreasing order of response performance as ZnO/CN > CN > ZnO. This study underscores the efficacy of valorization techniques in generating high-value carbon nanomaterials and their efficacy in bolstering gas sensor performance, with ZnO/CN demonstrating superior response capabilities.