bluuebunny/crossref_metadata_2025 huggingface dataset:
Seasonal Variability of Hydrological Parameters and Estimation of Circulation Patterns: Application to a Mediterranean Coastal Lagoon
Authors: Simantiris Nikolaos , Theocharis Alexander
Published: July 2024

Coastal lagoons are among the most important, but also threatened, marine systems of our planet. Rainfall, wind, seawater, and freshwater discharges control water circulation in lagoons, determining the water properties that are vital for the lagoon’s biodiversity. The present work is the first study on the circulation patterns and seasonal variability of hydrological parameters in Antinioti lagoon in western Greece, building a reference level on our knowledge of the hydrodynamic functioning of this marine ecosystem. This study shows that the lagoon’s water properties’ fluctuations and circulation variability are affected by an antagonistic effect between freshwater (river discharge, underground spring, rainfall) and seawater inputs. This effect, influenced by atmospheric forcing (rainfall, atmospheric temperature), controls the heat and salt budgets of the lagoon. Nevertheless, the lagoon keeps an almost balanced annual cycle, returning from June 2020 to June 2021 to similar values for all parameters.
Rapid On-Site Evaluation Performed by an Interventional Pulmonologist: A Single-Center Experience
Authors: Barisione Emanuela , Genova Carlo , Ferrando Matteo , Boggio Maurizio , Paudice Michele , Tagliabue Elena
Published: July 2024

Background: Rapid On-Site Evaluation (ROSE) during bronchoscopy allows us to assess sample adequacy for diagnosis and molecular analyses in the context of precision oncology. While extemporaneous smears are typically evaluated by pathologists, their presence during bronchoscopy is not always possible. Our aim is to assess the concordance between ROSE performed by interventional pulmonologists and cytopathologists. Methods: We performed ROSE on 133 samples collected from 108 patients who underwent bronchoscopy for the diagnosis of suspect thoracic findings or for mediastinal lymph node staging (May 2023–October 2023). Randomly selected smears (one for each collection site) were independently evaluated for adequacy by a pulmonologist and a pathologist to assess the concordance of their evaluation. Results: Among 133 selected smears evaluated by a pulmonologist and pathologist, 100 were adequate for both, 10 were inadequate for both and 23 were discordant; hence, global concordance was 82.7%; Cohen’s Kappa was 0.385, defining fair agreement. Concordance was similar irrespective of sample collection site (lymph nodes vs. pulmonary lesions; p = 0.999) and among samples which were considered adequate or inadequate by the pulmonologist (p = 0.608). Conclusions: Trained pulmonologists can evaluate the appropriateness of sampling with good concordance with cytopathologists. Our work supports autonomous ROSE by pulmonologists where pathologists are not immediately available.
A Multi-Omics Analysis of an Exhausted T Cells’ Molecular Signature in Pan-Cancer
Authors: Rigopoulos Christos , Georgakopoulos-Soares Ilias , Zaravinos Apostolos
Published: July 2024

T cells are essential tumor suppressors in cancer immunology, but their dysfunction induced by cancer cells can result in T cell exhaustion. Exhausted T cells (Tex) significantly influence the tumor immune environment, and thus, there is a need for their thorough investigation across different types of cancer. Here, we address the role of Tex cells in pan-cancer, focusing on the expression, mutations, methylation, immune infiltration, and drug sensitivity of a molecular signature comprising of the genes HAVCR2, CXCL13, LAG3, LAYN, TIGIT, and PDCD1across multiple cancer types, using bioinformatics analysis of TCGA data. Our analysis revealed that the Tex signature genes are differentially expressed across 14 cancer types, being correlated with patient survival outcomes, with distinct survival trends. Pathway analysis indicated that the Tex genes influence key cancer-related pathways, such as apoptosis, EMT, and DNA damage pathways. Immune infiltration analysis highlighted a positive correlation between Tex gene expression and immune cell infiltration in bladder cancer, while mutations in these genes were associated with specific immune cell enrichments in UCEC and SKCM. CNVs in Tex genes were widespread across cancers. We also highlight high LAYN methylation in most tumors and a negative correlation between methylation levels and immune cell infiltration in various cancers. Drug sensitivity analysis identified numerous correlations, with CXCL13 and HAVCR2 expressions influencing sensitivity to several drugs, including Apitolisib, Belinostat, and Docetaxel. Overall, these findings highlight the importance of reviving exhausted T cells to enhance the treatment efficacy to significantly boost anti-tumor immunity and achieve better clinical outcomes.
Point of Care Ultrasound (POCUS) in the Management of Heart Failure: A Narrative Review
Authors: Naddaf Nicki , Dianati Maleki Neda , Goldschmidt Marc E. , Kalogeropoulos Andreas P.
Published: July 2024

Assessing for volume overload is a key component of both short and long-term management of heart failure patients. Physical examination findings are neither sensitive nor specific for detecting congestion, and subclinical congestion may not be evident at the time of examination. Point of care ultrasound (POCUS) is an efficient and non-invasive way to assess heart failure patients for volume overload. The aim of our narrative review is to summarize how each of the following ultrasound modalities can be used to assess for congestion in the heart failure population: 2D and Doppler echocardiography, lung ultrasound, inferior vena cava ultrasound, internal jugular vein ultrasound, and venous excess grading. While each of these modalities has their limitations, their use in the acute and outpatient space offers the potential to reduce heart failure readmissions and mortality.
Algorithm-Based Modular Psychotherapy Alleviates Brain Inflammation in Generalized Anxiety Disorder
Authors: Kéri Szabolcs , Kancsev Alexander , Kelemen Oguz
Published: July 2024

Generalized anxiety disorder (GAD) is marked by prolonged and excessive worry, physical signs of anxiety, and associated neuroinflammation. Traditional treatments, like pharmacotherapy and cognitive–behavioral therapy (CBT), often leave residual symptoms and have high relapse rates. This study aimed to explore the efficacy of algorithm-based modular psychotherapy (MoBa), a combination of CBT and mindfulness meditation as validated by the research domain criteria (RDoC), in reducing anxiety and neuroinflammation in GAD. A longitudinal design was used, with 50 patients with GAD undergoing a 12-week MoBa treatment. The patients were investigated pre- and post-treatment using MRI to measure neuroinflammatory markers (DBSI-RF, diffusion-basis spectral imaging-based restricted fraction) in the hippocampus, amygdala, and neocortex. Clinical symptoms were assessed using the Hamilton Anxiety Rating Scale (HAM-A) and the Generalized Anxiety Disorder 7-item scale (GAD-7). Results indicated significant reductions in both anxiety symptoms and MRI RF values in the amygdala, suggesting decreased neuroinflammation. A reduction in anxiety was associated with the amelioration of neuroinflammation in the amygdala. These results suggest that MoBa is effective in alleviating both the psychological and neuroinflammatory aspects of GAD, offering a promising personalized treatment approach. Future research should focus on long-term effects and the mechanisms through which MoBa impacts neuroinflammation and anxiety.
Occurrence of Enterococci in the Process of Artisanal Cheesemaking and Their Antimicrobial Resistance
Authors: Hanzelová Zuzana , Dudriková Eva , Lovayová Viera , Výrostková Jana , Regecová Ivana , Zigo František , Bartáková Klára
Published: July 2024

Enterococci are a group of microorganisms that have a controversial position from some scientific points of view. The species of the greatest clinical importance are E. faecalis and E. faecium, which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, as they are used as non-starter lactic acid bacteria (NSLAB) in a variety of cheeses, especially artisanal cheeses. The aim of this study was to determine the presence of representatives from the Enterococcus genus using PCR and MALDI-TOF MS methods on samples of raw milk, processing environment swabs, and cheese from four different artisanal dairy plants in Slovakia. Among the 136 isolates of enterococci, 9 species of genus Enterococci (E. faecalis, E. faecium, E. durans, E. devriesi, E. hirae, E. italicus, E. casseliflavus, E. malodoratus, and E. gallinarum) were identified and were tested for their antimicrobial resistance (AMR) to 8 antibiotics (amoxicillin, penicillin, ampicillin, erythromycin, levofloxacin, vancomycin, rifampicin, and tetracycline); most of them were resistant to rifampicin (35.3%), ampicillin (22.8%), and tetracycline (19.9%). A PCR analysis of vanA (4.41%) and tetM (14.71%) revealed that antimicrobial resistance genes were present in not only phenotypic resistant isolates of enterococci but also susceptible isolates. The investigation of antimicrobial resistance in enterococci during the cheesemaking process can be a source of valuable information for public health in the concept of “One Health”.
Mathematics in Robot Control for Theoretical and Applied Problems
Authors: Mercorelli Paolo , Sergiyenko Oleg , Tsymbal Oleksandr
Published: July 2024

Technological development has not only boosted the use of mechanical systems for industrial uses but above all has made it possible for them to be used in areas and sectors unimaginable until a few years ago [...]
Advanced Therapies for Human Immunodeficiency Virus
Authors: Lindegger Daniel Josef
Published: July 2024

Human Immunodeficiency Virus (HIV) remains a significant global health challenge with approximately 38 million people currently having the virus worldwide. Despite advances in treatment development, the virus persists in the human population and still leads to new infections. The virus has a powerful ability to mutate and hide from the human immune system in reservoirs of the body. Current standard treatment with antiretroviral therapy effectively controls viral replication but requires lifelong adherence and does not eradicate the virus. This review explores the potential of Advanced Therapy Medicinal Products as novel therapeutic approaches to HIV, including cell therapy, immunisation strategies and gene therapy. Cell therapy, particularly chimeric antigen receptor T cell therapy, shows promise in preclinical studies for targeting and eliminating HIV-infected cells. Immunisation therapies, such as broadly neutralising antibodies are being investigated to control viral replication and reduce reservoirs. Despite setbacks in recent trials, vaccines remain a promising avenue for HIV therapy development. Gene therapy using technologies like CRISPR/Cas9 aims to modify cells to resist HIV infection or eliminate infected cells. Challenges such as off-target effects, delivery efficiency and ethical considerations persist in gene therapy for HIV. Future directions require further research to assess the safety and efficacy of emerging therapies in clinical trials. Combined approaches may be necessary to achieve complete elimination of the HIV reservoir. Overall, advanced therapies offer new hope for advancing HIV treatment and moving closer to a cure.
Effect of Silicon on the Martensitic Nucleation and Transformation of 301 Stainless Steel under Various Cold-Rolling Deformations
Authors: Li Jun , Li Yaji , Wang Jian , Han Peide
Published: July 2024

A systematic study was conducted on the influence of silicon on the microstructure, stress distribution, and martensitic nucleation and transformation of 301 metastable austenitic stainless steel during cold-rolling deformation. When the deformation amount of conventional 301 stainless steel is ≤20%, the amount of martensite transformation is very small. When the deformation amount is ≥30%, the amount of martensite transformation significantly increases. The introduction of Si significantly improves the amount of martensite transformation and the uniformity of deformation. 301Si-H has a significantly higher amount of martensite in the same deformation microstructure than conventional 301Si-L with a lower silicon content. Increasing the Si content decreases the stacking fault energy of 301 stainless steel. During deformation, Si tends to cluster at the grain boundaries, reducing stacking fault width and increasing dislocation density, creating sites for shear martensite nucleation at the grain boundaries. Simultaneously, significant deformation encourages the formation of deformation twins and facilitates martensite nucleation.
Oral Supplementation with Betaine Powder Ameliorated High Blood Pressure in Spontaneously Hypertensive Rats
Authors: Pelpolage Samanthi Wathsala , Sasaki Rie , Shimada Kenichiro , Nagura Taizo , Uchino Hirokatsu , Han Kyu-Ho , Fukushima Michihiro
Published: July 2024

Supplementation of betaine is associated with improved cardiac health, potentially due to its function in re-methylation of homocysteine, an independent risk factor for cardiovascular diseases. We investigated the effects of oral betaine supplementation on blood pressure homeostasis in spontaneously hypertensive (SHR) rats and Wistar Kyoto (WKY) rats in an 8 week-feeding trial with control (SHR-con and WKY-con) and 1% betaine supplemented (SHR-b and WKY-b) diets. Systolic, diastolic, and mean blood pressure in the SHR-b group were significantly lower at week 8 (p = 0.013, p = 0.011, p = 0.010, respectively). Furthermore, serum nitric oxide (NO) levels were significantly (p < 0.05) improved in the WKY-b and SHR-b groups, suggesting a healthy endothelial function. Additionally, the serum angiotensin I converting enzyme level in SHR-b rats was also significantly lowered, which may have been another reason for lower blood pressure. A significantly higher non-HDL level in the SHR-b group might reflect enhanced lipid secretion into the circulation in the form of very-low-density lipoprotein (VLDL). Betaine is known for its effect on the synthesis of phosphatidylcholine, a key component of VLDL. However, the long-term net outcomes of both blood pressure lowering and serum lipid increment should be further studied.
Conformal 3D Printing Algorithm for Surfaces and Its In Situ Repair Applications
Authors: Tang Jundong , Dong Yongli , Cai Lixiang , Zhu Qian , Shi Jianping
Published: July 2024

Conformal 3D printing can construct specific three-dimensional structures on the free-form surfaces of target objects, achieving in situ additive manufacturing and repair, making it one of the cutting-edge technologies in the current field of 3D printing. To further improve the repair efficacy in tissue engineering, this study proposes a conformal path planning algorithm for in situ printing in specific areas of the target object. By designing the conformal 3D printing algorithm and utilizing vector projection and other methods, coordinate transformation of the printing trajectory was achieved. The algorithm was validated, showing good adherence of the printing material to the target surface. In situ repair experiments were also conducted on human hands and pig tibia defect models, verifying the feasibility of this method and laying a foundation for further research in personalized medicine and tissue repair.
The Restriction Activity Investigation of Rv2528c, an Mrr-like Modification-Dependent Restriction Endonuclease from Mycobacterium tuberculosis
Authors: Liu Tong , Wei Wei , Xu Mingyan , Ren Qi , Liu Meikun , Pan Xuemei , Feng Fumin , Han Tiesheng , Gou Lixia
Published: July 2024

Mycobacterium tuberculosis (Mtb), as a typical intracellular pathogen, possesses several putative restriction–modification (R-M) systems, which restrict exogenous DNA’s entry, such as bacterial phage infection. Here, we investigate Rv2528c, a putative Mrr-like type IV restriction endonuclease (REase) from Mtb H37Rv, which is predicted to degrade methylated DNA that contains m6A, m5C, etc. Rv2528c shows significant cytotoxicity after being expressed in Escherichia coli BL21(DE3)pLysS strain. The Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL) assay indicates that Rv2528c cleaves genomic DNA in vivo. The plasmid transformation efficiency of BL21(DE3)pLysS strain harboring Rv2528c gene was obviously decreased after plasmids were in vitro methylated by commercial DNA methyltransferases such as M.EcoGII, M.HhaI, etc. These results are consistent with the characteristics of type IV REases. The in vitro DNA cleavage condition and the consensus cleavage/recognition site of Rv2528c still remain unclear, similar to that of most Mrr-family proteins. The possible reasons mentioned above and the potential role of Rv2528c for Mtb were discussed.
Long-Term Contaminant Exposure Alters Functional Potential and Species Composition of Soil Bacterial Communities in Gulf Coast Prairies
Authors: Lumibao Candice Y. , Liu Yue
Published: July 2024

Environmental pollution is a persistent threat to coastal ecosystems worldwide, adversely affecting soil microbiota. Soil microbial communities perform critical functions in many coastal processes, yet they are increasingly subject to oil and heavy metal pollution. Here, we assessed how small-scale contamination by oil and heavy metal impacts the diversity and functional potential of native soil bacterial communities in the gulf coast prairie dunes of a barrier island in South Texas along the northern Gulf of Mexico. We analyzed the bacterial community structure and their predicted functional profiles according to contaminant history and examined linkages between species diversity and functional potential. Overall, contaminants altered bacterial community compositions without affecting richness, leading to strongly distinct bacterial communities that were accompanied by shifts in functional potential, i.e., changes in predicted metabolic pathways across oiled, metal, and uncontaminated environments. We also observed that exposure to different contaminants can either lead to strengthened or decoupled linkages between species diversity and functional potential. Taken together, these findings indicate that bacterial communities might recover their diversity levels after contaminant exposure, but with consequent shifts in community composition and function. Furthermore, the trajectory of bacterial communities can depend on the nature or type of disturbance.
A CRISPR-Cas9-Mediated Large-Fragment Assembly Method for Cloning Genomes and Biosynthetic Gene Cluster
Authors: Guo Yujing , Cai Guang , Li Huiying , Lin Zhenquan , Shi Shuobo , Jin Jin , Liu Zihe
Published: July 2024

The ability to clone large DNA fragments from genomes is valuable for both basic and applied research, such as the construction of synthetic genomes, and the expression of biosynthetic gene clusters (BGCs) for natural product discovery. Here, we report a fast and efficient platform for the direct capture of genome DNAs, by combining CRISPR and Gibson assembly. We demonstrate this method with the ability of cloning large DNA fragments ranging from 30 to 77 kb from various host genomes, achieving a near 100% cloning fidelity for DNA fragments below 50 kb. We next demonstrate this method by the cloning of a 40 kb fragment from Streptomyces ceruleus A3(2), which is rich in BGCs for natural products; and used this method cloning the 40 kb fengycin synthetic gene cluster from B. subtilis 168, encoding for a class of peptides with bioactivity. This method provides efficient and simple opportunities for assembling large DNA constructs from distant sources.
Clarification of Distinguishing Natural Super-Reduced Phase from Synthetics Based on Inclusions
Authors: Ma Yutong , Miao Mengqi , Chen Ming , Qin Shan
Published: July 2024

Super-reduced phases (SRPs), such as silicon carbide (SiC) and metal silicides, have increasingly been reported in various geological environments. However, their origin remains controversial. SRP inclusions (e.g., metal silicides and metallic silicon (Si0)) within SiC are commonly believed to indicate a natural origin. Here, we identified an unusual SRP assemblage (SiC, (Fe,Ni)Si2, and Si0) in situ in an H5-type Jingshan ordinary chondrite. Simultaneously, our analysis showed that the SiC abrasives contain (Fe,Ni)Si2 and Si0 inclusions. Other inclusions in the artificial SiC were similar to those in natural SiC (moissanite) reported in reference data, including diverse metal silicides (e.g., FeSi, FeSi2, Fe3Si7, and Fe5Si3), as well as a light rare earth element-enriched SiO phase and Fe-Mn-Cr alloys. These inclusions were produced by the in situ reduction of silica and the interaction between Si-containing coke and hot metals during the synthesis of the SiC abrasives. The results demonstrate that the SRP assemblage in the Jingshan chondrite originates from abrasive contamination and that the SRP inclusions (with a low content of Ca, Al, Ti, and Zr) cannot be used as a conclusive indicator for natural SiC. Additionally, the morphologies, biaxiality, and polytypes (determined by Raman spectroscopy) of SiC abrasives bear resemblance to those reported for natural SiC, and caution must be exercised when identifying the origin of SRP in samples processed by conventional methods using SiC abrasives. At the end of this paper, we propose more direct and reliable methods for distinguishing between natural and synthetic SiC.
Development of Norelgestromin Dissolving Bilayer Microarray Patches for Sustained Release of Hormonal Contraceptive
Authors: Vora Lalitkumar K. , Tekko Ismaiel A. , Volpe Zanutto Fabiana , Sabri Akmal , Choy Robert K. M. , Mistilis Jessica , Kwarteng Priscilla , Kilbourne-Brook Maggie , Jarrahian Courtney , McCarthy Helen O. , Donnelly Ryan F.
Published: July 2024

Microarray patches (MAPs) offer a noninvasive and patient-friendly drug delivery method, suitable for self-administration, which is especially promising for low- and middle-income country settings. This study focuses on the development of dissolving bilayer MAPs loaded with norelgestromin (NGMN) as a first step towards developing a future potential drug delivery system for sustained hormonal contraception. The fabricated MAPs were designed with the appropriate needle lengths to penetrate the stratum corneum, while remaining minimally stimulating to dermal nociceptors. Ex vivo assessments showed that the MAPs delivered an average of 176 ± 60.9 μg of NGMN per MAP into excised neonatal porcine skin, representing 15.3 ± 5.3% of the loaded drug. In vivo pharmacokinetic analysis in Sprague Dawley rats demonstrated a Tmax of 4 h and a Cmax of 67.4 ± 20.1 ng/mL for the MAP-treated group, compared to a Tmax of 1 h and a Cmax of 700 ± 138 ng/mL for the intramuscular (IM) injection group, with a relative bioavailability of approximately 10% for the MAPs. The MAP-treated rats maintained plasma levels sufficient for therapeutic effects for up to 7 days after a single application. These results indicate the potential of NGMN-loaded dissolving bilayer MAPs, with further development focused on extending the release duration and improving bioavailability for prolonged contraceptive effects.
Comparative Efficacy of Inhaled and Intravenous Corticosteroids in Managing COVID-19-Related Acute Respiratory Distress Syndrome
Authors: Abdelkader Ahmed A. , Alsfouk Bshra A. , Saleh Asmaa , Abdelrahim Mohamed E. A. , Saeed Haitham
Published: July 2024

Acute respiratory distress syndrome (ARDS) is a life-threatening condition in which the lungs fail to provide sufficient oxygen to the body’s vital organs. It is commonly associated with COVID-19 patients. Severe cases of COVID-19 can lead to lung damage and organ failure due to an immune response in the body. To mitigate these effects, corticosteroids, which are known for their anti-inflammatory properties, have been suggested as a potential treatment option. The primary focus of this study was to assess the impact of various corticosteroid administration methods on the outcomes of patients with COVID-19. Methods: The current study was conducted on COVID-19 patients divided into three groups. The first group was administered 6 mg of intravenous (IV) dexamethasone; the second group received 1 mg/kg of IV methylprednisolone (methylprednisolone); and the third group received budesonide respirable solution at a dosage of 1mg twice daily. The neubilizer used was a vibrating mesh nebulizer (VMN). All patients received standard care. We found that dexamethasone administered intravenously led to a significant reduction in C-reactive protein levels, surpassing the effectiveness of both IV methylprednisolone and inhaled budesonide. Oxygen saturation without mask change over time showed statistically significant differences (p = 0.004) in favor of the budesonide and dexamethasone groups for all days. Individuals who received methylprednisolone showed a significant decrease in mortality rate and an extended survival duration, with statistical significance observed at p = 0.024. The rest of the parameters, including ferritin, lymphocytes, total leukocyte count, platelets, hemoglobin, urea, serum potassium, serum sodium, serum creatinine, serum glutamic-pyruvic transaminase, serum glutamic-oxaloacetic transaminase, uric acid, albumin, globulin, erythrocyte sedimentation rate, international normalized ratio, oxygen saturation with flow, and oxygen flow, showed no statistically significant differences between the three drugs. In conclusion, treatment with IV methylprednisolone (1 mg/kg) resulted in a shorter hospital stay, decreased reliance on ventilation, and improved health outcomes for COVID-19 patients compared to using dexamethasone at a daily dosage of 6 mg or budesonide respirable solution at a dosage of 1mg twice daily.
Exploring a New Generation of Pyrimidine and Pyridine Derivatives as Anti-Influenza Agents Targeting the Polymerase PA–PB1 Subunits Interaction
Authors: Giacchello Ilaria , Cianciusi Annarita , Bertagnin Chiara , Bonomini Anna , Francesconi Valeria , Mori Mattia , Carbone Anna , Musumeci Francesca , Loregian Arianna , Schenone Silvia
Published: July 2024

The limited range of available flu treatments due to virus mutations and drug resistance have prompted the search for new therapies. RNA-dependent RNA polymerase (RdRp) is a heterotrimeric complex of three subunits, i.e., polymerase acidic protein (PA) and polymerase basic proteins 1 and 2 (PB1 and PB2). It is widely recognized as one of the most promising anti-flu targets because of its critical role in influenza infection and high amino acid conservation. In particular, the disruption of RdRp complex assembly through protein–protein interaction (PPI) inhibition has emerged as a valuable strategy for discovering a new therapy. Our group previously identified the 3-cyano-4,6-diphenyl-pyridine core as a privileged scaffold for developing PA–PB1 PPI inhibitors. Encouraged by these findings, we synthesized a small library of pyridine and pyrimidine derivatives decorated with a thio-N-(m-tolyl)acetamide side chain (compounds 2a–n) or several amino acid groups (compounds 3a–n) at the C2 position. Interestingly, derivative 2d, characterized by a pyrimidine core and a phenyl and 4-chloro phenyl ring at the C4 and C6 positions, respectively, showed an IC50 value of 90.1 μM in PA–PB1 ELISA, an EC50 value of 2.8 μM in PRA, and a favorable cytotoxic profile, emerging as a significant breakthrough in the pursuit of new PPI inhibitors. A molecular modeling study was also completed as part of this project, allowing us to clarify the biological profile of these compounds.
Exploring the Role of Community Pharmacists in Pain Management: Enablers and Challenges
Authors: Mujtaba Syed Hassan , Gazerani Parisa
Published: July 2024

Pain is a common complaint, and the consumption of analgesics is prevalent. Community pharmacists, as primary contact points for patients, can play a crucial role in guiding patients toward rational pharmacotherapy or alternative pain management strategies. However, there are no specific educational curricula or standard guidelines to support this role, and the perception of this potential role is not well known. We conducted an anonymous online questionnaire among community pharmacists in Norway to assess their knowledge, perspectives, and willingness to engage in pain care. The survey also explored potential facilitators and barriers, and the use of any current guidelines. Seventy-one community pharmacists participated from various regions in Norway. Findings revealed that community pharmacists felt knowledgeable and willing to engage in pain management but anticipated barriers such as time constraints and a lack of standard guidelines. Participants also highlighted the need for better collaboration with other healthcare professionals and continuous professional development to enhance their role. To optimize the role of community pharmacists in pain management, therefore, integrating them into multidisciplinary healthcare teams, minimizing barriers, and providing continuous education and standard guidelines seem essential. This approach can empower community pharmacists and improve pain management outcomes.
Development and Validation of a Tool to Explore Attitudes Towards meDication adHErence Using a Novel Self-Reported QuestionnairE (ADHERE-7)
Authors: Bužančić Iva , Balen Mislav , Arbanas Dahna , Falamić Slaven , Fehir Šola Katarina , Galić Skoko Ana , Momčilović Mirna , Orbanić Ante , Tatarević Alena , Ortner Hadžiabdić Maja
Published: July 2024

Despite the availability of various tools for measuring medication adherence, efficiently identifying non-adherence levels and reasons at the point of care remains challenging. Existing tools often lack the ease of use needed for practical clinical application. This study aimed to develop and validate a user-friendly tool to provide healthcare professionals with a concise yet comprehensive means of identifying adherence behaviors. The methodology consisted of two phases: tool items were first developed using the nominal group technique with healthcare professionals, followed by a cross-sectional pilot study involving community-dwelling adults in Croatia. Validation analysis indicated acceptable face and content validity and satisfactory criterion validity, with Attitudes towards meDication adHErence self-Reported questionnairE (ADHERE-7) scores correlating with both the self-reported five-item Medication Adherence Report Scale (MARS-5 tool) (ρ = 0.765; p < 0.001) and an objective measure of the proportion of days covered (PDC) from pharmacy prescription claims data (G = 0.586; p = 0.015). Construct validity revealed three factors: Aversion, Comfort, and Practical Non-Adherence, with Cronbach’s alpha values of 0.617 for Aversion and 0.714 for Comfort Non-Adherence. The mean total score for ADHERE-7 was 26.27 ± 2.41 (range 17 to 28). This robust validation process confirms the ADHERE-7 tool as a reliable instrument for assessing medication adherence, addressing aversion, comfort, practical issues, and both intentional and unintentional nonadherence.
Conservation Potential Trough In Vitro Regeneration of Two Threatened Medicinal Plants Ungernia sewertzowii and U. victoris
Authors: Mustafina Feruza Usmanovna , Juraeva Hanifabonu Kobul kizi , Jamalova Dilafruz Nematilla kizi , Hazratov Abbos Tulkin ogli , Janabaeva Ayimxan Jalgasbaevna , Kim Hoe Jin , Na Chae Sun , Lee Min Sung , Oh Yu Jin , Tojibaev Komiljon Sharobiddinovich , Abdinazarov Sodikjon Kholiknazarovich
Published: July 2024

Ungernia sewertzowii (US) and U. victoris (UV) are medicinal plants and sources of biologically active compounds for pharmaceutical needs. The leaves of US contain 0.29–0.81% sum of alkaloids with a predominance of lycorine, which is 0.04–0.46% in leaves and 0.15–0.38% in bulbs. Lycorine is used to treat acute and chronic bronchitis. The leaves of UV contain 0.27–0.71% sum of alkaloids with a predominance of galanthamine—0.13–1.15%. Galanthamine is used to treat mild-to-moderate dementia (Alzheimer’s disease). The natural populations of US and UV are in danger as sources of income for local people. To resolve this problem, two protocols for microclonal propagation were developed to replace natural raw materials with in vitro regenerated plants. Callusogenesis of US and UV was induced on Murashige and Skoog (MS) nutrient media with 2.4D (0.5 mg/L) in combination with BAP (0.5 mg/L), Kin (0.5 mg/L), or Zea (0.5 mg/L). Direct (for US) and indirect (for US and UN) organogenesis were observed on MS with BAP (0.5 mg/L) or Kin (0.5 mg/L) in combination with IAA (0.5 mg/L) or NAA (0.5 mg/L). Direct organogenesis resulted in 3–5 bulbs of US on one explant; indirect organogenesis resulted in up to 100–150 bulbs of US and UV on one explant within 6 months, or five to six subcultures after transferring the callus to the nutrient medium. The tissue cultures of US and UV were characterized by very low data on antioxidant activity based on IC50 values for DPPH and ABTS radical scavenging activities, whereas in vitro regenerated plants (leaves and bulbs) had higher data. We concluded that in vitro regenerated plants are valuable sources of lycorine and galanthamine, which allow the protection of the natural populations of these two species from extinction.
In Vitro Hemostatic Activity of Novel Fish Gelatin–Alginate Sponge (FGAS) Prototype
Authors: Herliana Heri , Yusuf Harmas Yazid , Laviana Avi , Wandawa Ganesha , Abbas Basril
Published: July 2024

A hemostatic sponge prototype was successfully synthesized from fish gelatin as an alternative to mammalian gelatin; it was mixed with alginate in certain combinations, double cross-linked with calcium ions, and gamma irradiated at a dose of 20 kGy to improve the characteristics and effectiveness of its function as a local hemostatic agent. There were improvements in the physicochemical and mechanical properties, porosity index, absorption capacity, biodegradation properties, biocompatibility, and hemocompatibility of the fish gelatin–alginate sponge (FGAS) prototypes compared with the pure fish gelatin sponge. Hemostatic activity tests showed that the means for clotting time, prothrombin time, and activated partial thromboplastin time were shorter in the FGAS prototype than in the negative control, and there was no significant difference compared with the commercial gelatin sponge. The hemostatic mechanism of the FGAS prototype combined a passive mechanism as a concentrator factor and an active mechanism through the release of calcium ions as a coagulation factor in the coagulation cascade process.
Basalt Fibre-Reinforced Polymer Laminates with Eco-Friendly Bio Resin: A Comparative Study of Mechanical and Fracture Properties
Authors: Don Devmith Kariyawasam , Reiner Johannes , Jennings Matt , Subhani Mahbube
Published: July 2024

Fibre-reinforced polymers (FRPs) are widely used in industry due to their impressive strength-to-weight ratio, corrosion resistance and high durability. One of the primary components of FRPs is synthetic resins, specifically epoxy, which has been identified as harmful to the environment. To address this concern, an eco-friendly alternative made from basalt fibres and bio resin has the potential to reduce the environmental impact. This study investigates Basalt Fibre-Reinforced Polymer (BFRP) laminates manufactured using two bio resins, AMPRO™ BIO and Change Climate, comparing them to one conventional epoxy resin, WEST SYSTEM®, in terms of tensile modulus, strength and fracture toughness, as well as shear properties. The results indicate that BFRP laminates made with bio resins exhibit comparable or better mechanical properties to their conventional counterparts with tensile strength being between 6 and 17% more in bio resins compared to the conventional resin, thereby paving the way for further exploration of sustainable FRP laminates in future engineering applications.
Study on the Damage Evolution and Failure Mechanism of Floor Strata under Coupled Static-Dynamic Loading Disturbance
Authors: Li Hailong , Bai Haibo , Xu Wenjie , Li Bing , Qiu Peitao , Liu Ruixue
Published: July 2024

In the field test, we found that the failure depth of the goaf floor strata tends to be further because the periodic breaking and caving of the immediate roof, upper roof, and roof key stratum has dynamic stress disturbance effects on the floor. To further analyze its formation mechanism, this paper studies the damage evolution and fracture mechanism of goaf floor rock under the coupled static-dynamic loading disturbance caused by roof caving, based on the stress distribution state, the damage evolution equation of coal measure rock, the damage constitutive model, and the fracture criterion of floor rock. The main conclusions are listed as follows: 1. Based on the mining floor stress distribution, the floor beam model establishes the response mechanism of floor rock stress distribution. Also, the equation of stress distribution at any position in floor strata under mining dynamic load is given. 2. Combining the advantages of Bingham and the Generalized-Boydin model, the B-G damage constitutive model is established, which can describe the constitutive characteristics of coal measure rock under the coupled static-dynamic loading disturbance well. Furthermore, the variation law of parameters changing with strain rate is analyzed. 3. According to the twin-shear unified strength yield theory and the B-G damage constitutive model, coal measure rock’s twin-shear unified strength damage fracture criterion is established. Finally, the stress distribution expression of floor strata under concentrated and uniform dynamic loads is introduced, and the fracture criterion of goaf floor strata under a coupled static-dynamic loading disturbance is proposed.
Faith Leaders’ Perspectives on Involvement in HIV Prevention for Urban Black Youth in New Jersey, USA
Authors: Opara Ijeoma , Pierre Kimberly , Gabriel Cora , Cross Kristina , Clark Carolanne M. L. , Rutledge Jaleah D.
Published: July 2024

This qualitative study takes place in an urban community that has high rates of HIV among Black youth. Six faith leaders were interviewed (five identified as Christian and one identified as Muslim). Three major themes arose from the interviews, including (1) the role of sex and HIV; (2) hindrances to sexual health conversations with youth; and (3) considering religious principles to prevent HIV in Black youth. Findings from this study can be used to inform an HIV-prevention curriculum for Black youth who identify strongly with their religion and spirituality and live in high HIV-risk communities.
Black (and) Christian? New Systemic Racism and the ‘Refugee’ as a Depersonalised Category of Surplus: A Case Study of Tunisian Attitudes towards Sub-Saharan Africans
Authors: Zalta Anja , Krašovec Primož
Published: July 2024

This article is based on a months-long investigation and aims to contribute to the scientific understanding of the process of racialisation of the sub-Saharan migrants in Tunisia. The starting point of our research was the speech given by the Tunisian president, Kais Saied, in February 2023. In the light of new negotiations with the EU for technical, administrative, and financial support in the management of migration in the Mediterranean, the president emphasised the importance of Tunisia being and remaining Arab and Muslim. The sub-Saharan migrants who have penetrated the Mediterranean area in large numbers, mostly via Libya or Algeria, are black. Many of them are also Christians. The Tunisian case regarding the racialisation of migrants is similar to the dynamics of political discourses and actions of systemic racialisation in European countries. Our thesis is that racialisation based on religion and/or skin colour is part of a more complex dynamic, defined by the capitalist mode of production, which, due to its inner contradictions, simultaneously requires and expels human labour force. We claim that the permanently expelled constitute surplus populations that are, due to not being disciplined by the capitalist markets, considered dangerous, which is why they fall under police jurisdiction. This process of policing surplus populations is what constitutes contemporary systemic racism as a special mode of state politics, whereby “race” is the result of said process and not determined by its biological, religious, ethnic, or cultural characteristics. We support our thesis by a fieldwork study consisting of qualitative interviews with Tunisian experts, conducted based on purposive sampling and subsequent qualitative coding, as well as of three personal narrative interviews, which were conducted with sub-Saharan migrants from Cameroon, who had been living in a refugee “village” in the north of Tunisia for more than a year.
Study on the Religious and Philosophical Thoughts of Xizi Pagodas in Hunan Province of China
Authors: He Yiwen , He Lai , Zhou Qixuan , Xie Xubin
Published: July 2024

The Xizi Pagoda (惜字塔) is a form of traditional religious building used for burning “Paper with Characters (字纸)”, worshiping the gods, praying for blessings, revitalzing culture, and it is also an important material embodiment in the process of the development and evolution of religion in China, carrying China’s unique “Scholarly Culture (书香文化)”. This paper is the first study of the philosophy of religion of Xizi Pagodas (a vernacular stone religious architectural landscape). The research of the representative Xizi Pagodas in different parts of Hunan province were carried out to analyze the origin of Xizi Pagodas, cultural rituals, modeling paradigms, decorative art, and to explore the traditional Chinese religious and philosophical thoughts embedded in Xizi Pagodas through relevant literatures. The results show that Xizi Pagodas contain the religious concepts of “Confucianism, Buddhism and Taoism (儒释道)”: the emergence of Xizi Pagodas is connected to the development of Confucianism; Xizi Pagodas incorporate Buddhistic thoughts and inherit architectural features of Buddhist Pagodas; and the “Xizi (惜字)” ritual and images of deities on Xizi Pagodas are associated with Taoism. At present, the number of Xizi Pagodas is decreasing, and the remaining ones are in a serious state of dilapidation and disrepair, and the “Xizi” Culture is gradually being forgotten and fading away. This paper enriches the relevant research on the Xizi Pagodas and the “Xizi” Culture.
A New Approach to Build a Successful Straddle Strategy: The Analytical Option Navigator
Authors: Rustamov Orkhan , Aliyev Fuzuli , Ajayi Richard , Suleymanov Elchin
Published: July 2024

The study described in this paper develops a new technique which permits the execution of an open straddle strategy based on the superior volatility forecast for analyzing historical data. We extend the current litearure by measuring the volatility of an underlying asset in the last predefined period and comparing the actual volatility in currency with historical volatility in currency to make predictions of implied volatility. We calculated stock price volatility through an optimal holding period (OHP) and set up bars of volatility in currency. To obtain this, we solved optimization equations to find maximum and minimum movements in the volatility in currency within the defined range. We placed volatility in currency into percentile rankings and designed a straddle trading strategy based on the last OHP’s volatility in currency. The technique allows for an investor (or trader) to open either short or long positions based on calculations for a selected OHP’s volatility in currency. We applied this strategy to 130 stocks which are traded on CBOE. We developed a trading algorithm which can be used by institutional as well as individual investors. The algorithm is set to determine historical volatility in currency and forecast upcoming volatilities in currency through the understanding of the market sentiment. The empirical findings show that the stocks analyzed with the algorithm generate positive returns along a spectrum of changing volatilities of the underlying assets.
Entrepreneurship Ecosystem of Cooperatives in Mexico City
Authors: Díaz de León Denise , Rivera Igor , Álvarez Edgar Rogelio
Published: July 2024

This study enhances our understanding of entrepreneurial ecosystems in Mexico City, emphasizing their crucial roles in fostering cooperative activity within specific contexts. It delves into the intricate interplay of ecosystem elements and their interconnectedness, shedding light on how it shapes entrepreneurial ventures in the region. We used a qualitative methodological approach and conducted semi-structured interviews defined from theoretical analysis and snowball sampling to identify key local actors, how they interact, and what obstacles they face. Our data show that the ecosystem comprises many actors, such as social economy organizations, civil society, beneficiaries, and alternative markets, as well as such diverse elements as the regulatory framework, public policies, and financing programs. The actors who have the most impact and work most consistently with cooperatives are the academy, cooperative unions, and government entities. All of them actively interact with each other, but we underscore the need for greater dynamism to enhance entrepreneurial activity.
Prevalence of Specific Mood Profile Clusters among Elite and Youth Athletes at a Brazilian Sports Club
Authors: de Miranda Rohlfs Izabel Cristina Provenza , Noce Franco , Wilke Carolina , Terry Victoria R. , Parsons-Smith Renée L. , Terry Peter C.
Published: July 2024

Those responsible for elite and youth athletes are increasingly aware of the need to balance the quest for superior performance with the need to protect the physical and psychological wellbeing of athletes. As a result, regular assessment of risks to mental health is a common feature in sports organisations. In the present study, the Brazil Mood Scale (BRAMS) was administered to 898 athletes (387 female, 511 male, age range: 12–44 years) at a leading sports club in Rio de Janeiro using either “past week” or “right now” response timeframes. Using seeded k-means cluster analysis, six distinct mood profile clusters were identified, referred to as the iceberg, surface, submerged, shark fin, inverse iceberg, and inverse Everest profiles. The latter three profiles, which are associated with varying degrees of increased risk to mental health, were reported by 238 athletes (26.5%). The prevalence of these three mood clusters varied according to the response timeframe (past week > right now) and the sex of the athletes (female > male). The prevalence of the iceberg profile varied by athlete sex (male > female), and age (12–17 years > 18+ years). Findings supported use of the BRAMS as a screening tool for the risk of psychological issues among athletes in Brazilian sports organisations.
Rank-In Integrated Machine Learning and Bioinformatic Analysis Identified the Key Genes in HFPO-DA (GenX) Exposure to Human, Mouse, and Rat Organisms
Authors: Li Xinyang , Xiao Hua , Zhu Liye , Liu Qisijing , Zhang Bowei , Wang Jin , Wu Jing , Song Yaxiong , Wang Shuo
Published: July 2024

Hexafluoropropylene Oxide Dimer Acid (HFPO-DA or GenX) is a pervasive perfluorinated compound with scant understood toxic effects. Toxicological studies on GenX have been conducted using animal models. To research deeper into the potential toxicity of GenX in humans and animals, we undertook a comprehensive analysis of transcriptome datasets across different species. A rank-in approach was utilized to merge different transcriptome datasets, and machine learning algorithms were employed to identify key genetic mechanisms common among various species and humans. We identified seven genes—TTR, ATP6V1B1, EPHX1, ITIH3, ATXN10, UBXN1, and HPX—as potential variables for classification of GenX-exposed samples, and the seven genes were verified in separate datasets of human, mouse, and rat samples. Bioinformatic analysis of the gene dataset further revealed that mitochondrial function and metabolic processes may be modulated by GenX through these key genes. Our findings provide insights into the underlying genetic mechanisms and toxicological impacts of GenX exposure across different species and offer valuable references for future studies using animal models to examine human exposure to GenX.