bluuebunny/crossref_metadata_2025 huggingface dataset:
Pancreatic β-cell specific deletion of VPS41 causes diabetes due to defects in insulin secretion
Authors: Admin Ada , Burns Christian H. , Yau Belinda , Rodriguez Anjelica , Triplett Jenna , Maslar Drew , An You Sun , Welle Reini E.N. van der , Kossina Ross G. , Fisher Max R. , Strout Gregory W. , Bayguinov Peter O. , Veenendaal Tineke , Chitayat David , Fitzpatrick James A.J. , Klumperman Judith , Kebede Melkam A. , Asensio Cedric S.
Published: November 2020

Insulin secretory granules (SGs) mediate the regulated
secretion of insulin, which is essential for glucose homeostasis. The basic
machinery responsible for this regulated exocytosis consists of specific
proteins present both at the plasma membrane and on insulin SGs. The protein
composition of insulin SGs thus dictates their release properties, yet the
mechanisms controlling insulin SG formation, which determines this molecular
composition, remain poorly understood. VPS41, a component of the endo-lysosomal
tethering HOPS complex, was recently identified as a cytosolic factor involved in the formation of neuroendocrine and neuronal granules. We
now find that VPS41 is required for insulin SG biogenesis and regulated insulin
secretion. Loss of VPS41 in pancreatic b-cells leads to a reduction
in insulin SG number, changes in their transmembrane protein composition, and
defects in granule regulated exocytosis. Exploring a human point mutation,
identified in patients with neurological but no endocrine defects, we show that
the effect on SG formation is independent of HOPS complex formation. Finally,
we report that mice with a deletion of VPS41 specifically in β-cells develop diabetes due to severe
depletion of insulin SG content and a defect in insulin secretion. In sum, our data demonstrate that VPS41 contributes to
glucose homeostasis and metabolism.
Zwischen den Welten. Archäologie einer europäischen Grenzregion zwischen Sachsen, Slawen, Franken und Wikingern
Authors: Schneeweiß Jens
Published: November 2020

Im Zentrum des Buches steht der Höhbeck/Elbe an der Grenze zwischen Niedersachsen und Brandenburg, wo zur Wikingerzeit Welten aufeinandertrafen und sich eine Zeit lang europäische Geschichte verdichtete. Im Frühmittelalter waren die Wikinger die "global Player" des Nordens, den Westen bestimmte Karl der Große und sein Reich, gefolgt von den Ottonen. Im östlichen Europa lag die Welt der Slawen. Vom 8.–11. Jahrhundert stießen diese Welten dort aneinander, wo im 20. Jahrhundert der Eiserne Vorhang Europa teilte. Von 2005–2009 wurden in dieser Grenzregion an der Elbe umfangreiche archäologische Ausgrabungen vorgenommen. Die Ergebnisse bilden die Grundlage des Buches, das vom ersten Auftreten von Slawen an der Elbe bis zum Beginn des hochmittelalterlichen Landesausbaus reicht. Hier lag der untergegangene karolingische Grenzhandelskontrollort Schezla mit dem castellum hohbuoki, hier besiegte Heinrich I. die Slawen in einer bedeutenden Schlacht. Ausdruck der sensiblen Mensch-Umwelt-Beziehungen ist die enge Bindung der Siedlungsentwicklung an die Dynamik der Flusslandschaft, die mit dazu beitrug, dass die Geschichte am Höhbeck in eine Sackgasse lief. Der nachfolgende Bedeutungsverlust dieser Region ermöglicht uns heute einen überraschend unverbauten Einblick in diesen Zeitraum, wie er nur ausnahmsweise zu bekommen ist. Eine interdisziplinäre Herangehensweise ermöglicht die Darstellung verschiedener Perspektiven auf die Siedlungs- und Ereignisgeschichte. Neben traditioneller archäologischer Fund- und Befundanalyse enthält der Band geoarchäologische, historische und theoretische Annäherungen, wodurch neue Interpretationsebenen erschlossen werden.
Kinetic Investigations of Phaseformation Processes in the BaO-Al2O3-Fe2O3 System
Authors:   Ukrainian Engineering Pedagogic Academy Department of Chemistry, Chemical and Food Technology, Iloukha Nikolay , Barsova Zoya ,   Ukrainian Engineering Pedagogic Academy Department of Chemistry, Chemical and Food Technology, Cwhanovskaya Irina ,   Ukrainian Engineering Pedagogic Academy Department of Chemistry, Chemical and Food Technology, Timofeeva Valentina ,   Ukrainian Engineering Pedagogic Academy Department of Chemistry, Chemical and Food Technology
Published: November 2020

The kinetic investigations of phase formation processes in the mixture which contains BaO, Al2O3, Fe2O3 were carried out. The degree of conversion and the activation energy were calculated, the dependence of the reaction rate and the rate constant on reaction temperature was determined.
Masking is the Effective Alternative to the Separation during Osmium Determination by Means of Azo Dyes in Complex Samples
Authors:   Ivan Franko National University of Lviv, Kyryla i Mefodiya Str., 6, 79005, Lviv, Ukraine, Rydchuk Mariana , Vrublevska Teodoziya ,   Ivan Franko National University of Lviv, Kyryla i Mefodiya Str., 6, 79005, Lviv, Ukraine, Boyko Mariya ,   Ivan Franko National University of Lviv, Kyryla i Mefodiya Str., 6, 79005, Lviv, Ukraine, Korkuna Olha ,   Ivan Franko National University of Lviv, Kyryla i Mefodiya Str., 6, 79005, Lviv, Ukraine
Published: November 2020

The selectivity of the spectrophotometric methods for determination of microgram quantities of osmium (IV) with acidic monoazo dyes Tropaeolin O (TpO) and Tropaeolin OOO-I (TpOOOI) in the presence of concomitant metals, particularly platinum group, heavy and rare earths (RE) has been studied. The tolerance ratios of interfering elements have been established. The selectivity of the interaction of TpOOOI with osmium (IV) relatively to platinum group elements (PGEs) is much better than TpO. It has been established that the influence of interfering ions can be easily eliminated by means of masking agents viz. EDTA, tartrate, citrate, pyrophosphate and fluoride, and thereby the selectivity of the methods of osmium determination with TpO and TpOOOI has been sufficiently improved. The methods have been approved during the analyses of various model solutions containing ruthenium, platinum, palladium, rhodium and iridium and the masking reagents. Also osmium content has been determined in the intermetallic alloy Nd20Os15Sі65. The standard deviations did not exceed typical values for spectrophotometric methods (RSDs < 5 %). Thus the elaborated methods with various masking agents may be used for direct determination of osmium in complex samples without any preliminary separation of osmium in the form of OsO4 as well as without the extraction of matrices elements.
Problems of Wastewater Pretreatment from the Edible Oils Production
Authors:   University of Bielsko-Biala, Faculty of Materials and Environmental Sciences, Institute of Environmental Protection and Engineering 2, Willowa str., 43-309 Bielsko-Biala, Poland, Przywara Lucyna
Published: November 2020

The pretreatment of the wastewater from the edible production based on calcium phosphates precipitation, generates sludge characterized by good sedimenting properties. However, a part of the sludge floats through the surface of the settling tank. Lime added for phosphorus removal simultaneously removes also sulphates to a level of above 2000 mg/l. The liquid associated with floated sludge contains low sulfates concentration – about 370 mg/l. The aim of the research project was to examine the causes of such a low sulphates concentration.
Correlation between Electrochemical Corrosion and Structural State of Steel by Simulation of Operation Conditions of Railway Wheels
Authors:   National Metallurgical Academy of Ukraine, Gagarin ave., 4, Dnepropetrovsk-5, 49600, Ukraine, Pinchuk Sofia , Gubenko Svetlana ,   National Metallurgical Academy of Ukraine, Gagarin ave., 4, Dnepropetrovsk-5, 49600, Ukraine, Belaya Elena ,   National Metallurgical Academy of Ukraine, Gagarin ave., 4, Dnepropetrovsk-5, 49600, Ukraine
Published: November 2020

Corrosion mechanism and corrosion rate of wheel steel of different chemical and structural compositions have been investigated. It has been shown that “white layers”, variation in grains size and structure streakiness of wheel steel increase corrosion rate. Wheel steel with vanadium additives corrodes more quickly than that without vanadium. Non-metallic inclusions are the centers of corrosion nucleation and their influence on corrosion depends on inclusion type. Corrosion mechanism of wheel steel has been discussed.
Múltiplos e divisores: uma experiência com o uso do jogo de trilhas
Authors: Caldas Fernando da Silva , Graça Vagner Viana da , Risuenho Marques Valéria
Published: November 2020

O presente trabalho tem por objetivo relatar o uso de um jogo matemático de trilhas de MMC e MDC, envolvendo conceitos de múltiplos e divisores de um número natural em uma turma do 6º ano do ensino fundamental de uma escola pública pertencente à Secretaria Municipal de Educação da cidade de Belém – PA. Para tanto, aplicou-se um teste diagnóstico com os alunos do 7º ano do ensino fundamental, egressos do 6º ano do ensino fundamental, em relação aos conceitos de múltiplos e divisores de um número natural. A partir dessa informação, realizamos um estudo bibliográfico sobre as dificuldades verificadas. Por fim, elaborou-se e aplicou-se uma intervenção pedagógica com alunos de uma turma do 6º ano do ensino fundamental, por meio da utilização do jogo "Trilha do MDC e do MMC”. Após a aplicação do jogo percebemos maior envolvimento dos alunos, concentração e determinação em relação à resolução dos desafios que se colocavam. Tal percepção foi fundamental para compreendermos que precisamos diversificar as estratégias e metodologias para o trabalho com os conteúdos de matemática no Ensino Fundamental.
Dakwah Religi di Media TV pada Bulan Ramadhan dalam Perspektif Psikologi Islam
Authors: Saliyo Saliyo
Published: November 2020

Artikel ini merupakan hasil  penelitian pendampingan dakwah religi dimedia TV pada bulan Ramadhan di Simpang 5 TV Pati dan Jawa Tengah dalam perspektif psikologi Islam.  Penelitian tersebut memiliki tujuan untuk mengetahui permasalahan psikologis serta memberikan motivasi pada daâ€™i dan daâ€™iyat ketika melakukan dakwah di media TV. Tujuan yang lain juga untuk mengetahui efektifitas dakwah religi di media TV.  Metode yang digunakan dalam penelitian pemberdayaan dakwah religi adalah Participatory Action Research (PAR). Untuk melaksanakan  Participatory Action Research (PAR) ada enam langkah yang perlu dilaksanakan oleh peneliti. Langkah langkah tersebut adalah learning in action, exploring problems, fact,finding and listening, knowing the actors,   assessing option, dan understanding system. Hasil penelitian menunjukan bahwa permasalahan psikologis para daâ€™i dan daiâ€™yat yang belum pernah dakwah religi di TV ketika hendak berdakwah di TV adalah permasalahan kecemasan, minder, kepercayaan diri dan permasalahan psikologis lainnya. Seiring dengan kegiatan pendampingan pemberdayaan serta motivasi peneliti dengan menggunakan  ilmu psikologi Islam permasalahan tersebut dapat teratasinya. Caranya orang tersebut diminta untuk berdoa sebelum tampil di depan kamera serta banyak latihan sebelumnya. Kelebihan dakwah religi di TV dapat disaksikan  oleh orang banyak. Kekurangannya dakwah religi di TV bagi pemirsanya dan daâ€™i dan daâ€™iyat tidak dapat berinteraksi dengan banyak teman yang menyaksikan dan menikmati dakwah tersebut.
The AA344 and AA345 antibodies detect human tubulin by immunofluorescence in HeLa cells
Authors: Lima Wanessa , Cosson Pierre
Published: November 2020

The AA344 and AA345 antibodies detects by immunofluorescence human tubulin in methanol-fixed HeLa cells.
RB155, RB156 and RB189 antibodies do not recognize the D. discoideum Tsg101 protein by western blot
Authors: Leuba Florence , Soldati Thierry
Published: November 2020

The recombinant antibodies RB155, RB156 and RB189 do not detect by western blot the full-length Tsg101 protein from Dictyostelium discoideum.
The AJ517 antibody labels the mouse CD8β protein by western blot
Authors: Ameti Ilhan , Hussami Sara , Maxit Orlane , Spedaliero Sébastien , Gaspoz Vincent , Giusti Teresa , Manoukian Arec , Bulla Monica , Sassi Ali , Guilhen Cyril
Published: November 2020

The AJ517 antibody detects the mouse CD8β protein by western blot.
The AL626 antibody recognizes an ALFA-tagged protein by western blot
Authors: Lamrabet Otmane
Published: November 2020

The AL626 antibody against the ALFA tag detects an ALFA-tagged human TAC protein by western blot.
Citrus TISSUE CULTURE WITH TWO DIFFERENT APPROACHES
Authors: Chamandoosti Firoozeh
Published: November 2020

Citrus is an important genius for economy and human health but a susceptible genius against biotic and abiotic stresses, so it needs to improved programs. Different basal media (MS, ½MA, ?MS and DKW) and different kind and concentrations of plant growth regulators i.e. BA, KIN, 2ip, ZE and TDZ (0 – 2 mg/l) and NAA, IAA and IBA (0 – 2 mg/l) added with 30 g/l sucrose, 3 g/l active charcoal and 7.5 g/l bacteriological agar] were used for organogenesis include shooting and rooting, also callusing from nodal explant of Citrus latifolia. MS medium supplemented with 1 mg/l BA, and 0.01 mg/l NAA is the best media for multiple shoot induction on nodal explants and elongation of them. Other cytokinins had not significant effects on shoot induction and multiplication. Using of 0.01 mg/l IBA instead of 0.01 mg/l NAA on medium with 1 mg/l BA, led to multiple shoot induction on nodal explant indirectly. Rooting was induced on DKW medium plus 1.5 mg/l NAA in the best way compared another media. Both direct and indirect organogenesis (multiple shoot induction) were carried out on media with very similar contents. So we can use very simple and practical methods tissue culture for different improved programs in Citrus genius genius.
Biocompatible and biodegradable wound dressings on the basis of seaweed polysaccharides (review of literature)
Authors: Kuznetsova T. A. , Besednova N. N. , Usov V. V. , Andryukov B. G.
Published: November 2020

The review presents the characteristics of modern biocompatible and biodegradable wound dressings on the basis of seaweed polysaccharides (carrageenans of red algae, fucoidans and alginates of brown algae, ulvans of green algae) and notes the key physicochemical and biological properties that are important for constructing wounds dressings. There are information on various types of wound dressings and results of experimental and clinical tests of dressings in the treatment of wounds of various origins. Particular attention is paid to hydrogel dressings, since hydrogels meet the basic requirements for an ideal wound dressing, and many marine polysaccharides are able to form hydrogels.
Transthoracic Lung node biopsy under navigation of C-arm based universal angiographic complex
Authors: Baryshnikov I. S. , Egorova A. O. , Polezhaev Dmitry Alexandrovich
Published: November 2020

The objective was to study the possibilities of using the C-arm based universal angiographic complex as a navigation system in transthoracic biopsies of pulmonary nodules.

Methods and materials. The study is based on the data of 60 patients with lesion size from 10 to 103 mm (mean 51 mm) have been underwent the transthoracic biopsy using C-arm based universal angiographic complex navigation. A total of 69 biopsies were performed (60 primary, 9 repeated). The procedure was made with local anesthesia in an interventional operating room. The postoperative observation time was 1 day.

Results. A tissue sample was got in all cases. As a result of a biopsy, a malignant tumor was confirmed in 70 % of patients (42 people). At the primary biopsy, data for the malignant process were not obtained in 30 % (18 people), however, in half of the cases (9 people), the diagnosis of a malignant tumor was confirmed after repeated biopsy. The radiation dose received by the patient was 2.05-60.50 mSv (mean 13.94mSv), which is comparable to the radiation dose in traditional CT navigation and less than the average dose of radiation obtained using CT fluoroscopy. Sensitivity, specificity, accuracy were 82.35, 100 и 35 % respectively. Complications occurred in 18 cases (26.09 %). There was no need to perform interventions under general anesthesia. Transfer of patients to the intensive care unit was not required. No fatal complications occurred.

Conclusion. This navigation system is safe and effective and can be used routinely.
Small bowel hemangioma in a 7-years-old boy, complicated by bleeding
Authors: Gavrilyuk V. P. , Donskaja E. V. , Severinov D. A.
Published: November 2020

Small intestine hemangioma is a benign vascular tumor formation, accounts for 0.05 to 0.3 % of all gastrointestinal tumors. This article presents a rare clinical observation - intraluminal hemangioma of distal parts of the ileum in a boy of 7 years old, complicated by massive bleeding. The presented clinical observation shows the difficulty of diagnosis, as well as the preliminary diagnosis performed by a pediatric surgeon: Meckel’s diverticulum, complicated by bleeding. As we know, more than 70 % of cases of gastrointestinal bleeding in children are really associated with the pathology of Meckel’s diverticulum. This requires the transfer of such patients to a surgical hospital, performing surgical intervention for revision of the abdominal cavity and excision of the pathological formation, as well as additional surgical techniques depending on intraoperative tactics.The described case demonstrates effective diagnostic tactics and features of intraoperative picture, surgical treatment of patient based on laparotomy and resection of intestinal area. The possibility of achieving encouraging results of surgical treatment of this difficult category of patients with reasonable choice of surgical tactics in childhood has been demonstrated. It is obvious that the key to successful treatment of such patients is timely diagnosis, which is not easy task due to the morphological and topographic characteristics of the pathological focus.
Adolescent Perceptions of Injury and Pressures of Returning to Sport: A Retrospective Qualitative Analysis
Authors: West Jordan , Winkelmann Zachary , Edler Jessica , Jackson Bradley , Eberman Lindsey
Published: November 2020

The increase in sport participation among adolescents has led to the rise in sport-related injuries, many of which have unique characteristics based on the patient, their perceptions, and the pressures faced when returning to sport. The purpose of this study was to identify the underlying factors that contributed to adolescents’ perceptions of injury and the various pressures they experienced when returning to sport. Two themes emerged from the study: support and fear. Support was provided to participants through development, care, and the environment. Fear was the factor that affected the participant in their return to sport, which came in the form of worry and doubt.
INTERAKSI PERLAKUAN MIKORIZA DAN INOKULUM Rhizobium sp TERHADAP PERTUMBUHAN DAN PEMBENTUKAN BINTIL AKAR Mucuna bracteata
Authors: Wahyuni Mardiana , Saragih Ricky Elmando , Sembiring Mariani
Published: November 2020

Tujuan penelitian dilakukan untuk mengetahui pengaruh perlakuan Mikoriza dan inokulasi Rhizobium sp terhadap pertumbuhan dan pembentukan bintil akar Mucuna bracteata.Penelitian dilaksanakan di kebun percobaan STIPAP Medan. Penelitian dilakukan pada bulan April â€“ Juli 2019. Penelitian menggunakan metode Rancangan Acak Kelompok (RAK) 2 Faktorial terdiri dari 3 dan 3 perlakuan dengan 4 ulangan, sehingga total sampel keseluruhan 36 bibit. Parameter yang diamati adalah panjang sulur, jumlah daun, jumlah bintil akar, berat kering bintil akar, kadar klorofil, kadar N daun dan derajat infeksi akar. Data yang diperoleh dianalisis secara statistik dengan analisis of variance (ANOVA) dengan uji beda nyata 5%. Hasil penelitian menunjukkan bahwa perlakuan tunggal terbaik untuk Mikoriza pada penelitian ini adalah M2 dengan dosis 40 g/bibit. Aplikasi Rhizobium sp berpengaruh nyata terhadap panjang sulur, jumlah daun, jumlah bintil akar pada perlakuan tunggal terbaik adalah R2 dengan dosis 0,6 ml/bibit. Interaksi perlakuan Mikoriza dan Rhizobium sp menunjukkan pengaruh yang nyata pada perlakuan M2R2 yaitu aplikasi Mikoriza dengan dosis 40 g/bibit dan aplikasi Rhizobium sp dengan dosis 0,6 ml/bibit memiliki pertumbuhan terbaik untuk beberapa parameter pengamatan.
METHOD FOR DRYING MAIZE POLLEN
Authors: Fariroh Indri , Rusdiana Riza Yuli
Published: November 2020

Pollen managements consisted of suitable harvest timing, pollen drying to a certain misture content, pollen viability testing, and pollen storage. Maize pollen was sensitive to desiccation. This experiment was aimed to determine preservation technique in reducing pollen water content while maintaining its viability. Maize variety which used in this research was BIMA 3 as male line hybrid maize. Pollen viability testing used potassium iodide (KI) 1%. This experiment used randomized block design with 3 factors and 2 replicates, i.e. boron (H3BO3) fertilizers (0 and 3 kg ha-1 ), drying techniques (MgCl2, silica gel, zeolite, preservation in air-cond room (18Â±1 Â°C; RH43%), and drying periods (0, 2, 4, 6, 8 hours). The observation response of this experiment were viability and pollen weight changing. The result showed that there is no interaction between three factors to observation response, while drying technique and drying period combinations showed interactions to observation response. Pollen maize drying used silica gel for 4 hours was able to decline much pollen weight and maintain pollen viability better than other techniques.
O governo das armas: a administração dos terços auxiliares e ordenanças na Capitania de Pernambuco e a organização militar da Comarca de Alagoas (c. 1729-c. 1759)
Authors: Santos Everton Rosendo
Published: November 2020

O presente artigo visa a analisar a estrutura do sistema defensivo vivenciada na Comarca de Alagoas na Capitania de Pernambuco, a partir de Instruções Régias destinadas aos corpos de ordenanças e auxiliares no período entre os governos dos monarcas D. João V e D. José I (1729-1759). Além disso, objetiva-se compreender as formas de promoção social, e as possibilidades de mobilidade entre essas duas organizações distintas. Ademais, buscaremosentender nesta investigação como as alterações recomendadas pelas autoridades régias foramexecutadas pelos governadores e capitães-generais da capitania de Pernambuco. Para tanto, foram analisados manuscritos provenientes do Arquivo Público Estadual Jordão Emerenciano e do Arquivo Histórico Ultramarino.
Ser nobre na capitania do Rio Grande: Formação de uma conhecida nobreza da terra (1660 – 1760)
Authors: Barbosa Kleyson Bruno Chaves
Published: November 2020

O que era ser nobre nas conquistas portuguesas da América? Existiam aqueles que de fato eram nobres titulados pelo rei e, aqueles que se portavam e se entendiam enquanto nobres, forjando-se como uma nobreza da terra. Estudos sobre Olinda, Rio de Janeiro e São Luís do Maranhão demonstram a vinculação entre a ocupação de cargos camarários, obtenção demercês e postos militares, bem como um discurso de antiguidade, tradição e esforço “à custa de vida, sangue e fazenda” para a composição das chamadas nobrezas da terra. Neste artigo, buscou-se compreender, a partir do contexto político e social da capitania do Rio Grande, sobre como um poder local desta espacialidade também se assemelhava em seumodo de agir observado para outras governanças locais. Dessa forma, questiona-se sobre o que era ser nobre ou as possibilidades de viver ao modo nobre na capitania do Rio Grande, assim como se analisa a formação de uma “conhecida nobreza da terra”, radicada nos postos camarários e de ordenanças e gestada durante os conflitos da Guerra dos Bárbaros, entre a segunda metade do século XVII e a primeira metade do século XVIII. Para isso, realizou-se um trabalho de análise bibliográfica, aliado ao método prosopográfico, a partir da documentação produzida pela Câmara de Natal, como os termos de vereações e os livros de registros de cartas e provisões, bem como as fontes paroquiais, a fim de se entender quem eram essas pessoas, famílias, e a chamada nobreza da terra.
Os ouvidores régios e os poderes locais no Ceará setecentista
Authors: Carvalho Reinaldo Forte
Published: November 2020

Este estudo analisa a consolidação do poder local no controle político e administrativo da Capitania do Ceará no setecentos. A análise prioriza as práticas políticas empregadas pelos potentados locais como forma de preservação hegemônica do poder frente aos dispositivos normativos da administração colonial portuguesa na Capitania do Ceará. O estudo é ancorado no campo teórico da Nova História Política, com base na matriz conceitual da História do poder político, disposta nos temas da história dos poderes locais e da administração da justiça colonial que norteiam todo o estudo. O objetivo central é analisar as relações de poder entre os potentados locais e os representantes da administração da justiça na Capitania do Ceará no Século XVIII. Especificamente o estudo caracteriza os principais aspectos da Capitania do Ceará no processo de consolidação da administração colonial portuguesa, identificando a constituição do poder local no Ceará e da instituição dos poderes formais da administração colonial na referida Capitania. Objetiva também, investigar as relações de força entre os potentados locais e os representantes da administração portuguesa em meio aos conflitos de jurisdição no controle administrativo da Capitania do Ceará.
Mercados, guerras e mercês: a mobilidade social no antigo regime através da trajetória e Ambrósio Fernandes Brandão, mercador, cristão-novo nas capitanias do Norte
Authors: Menezes Mozart Vergetti de , Brandão Maria Eduarda Medeiros
Published: November 2020

O Antigo Regime testemunhou transformações de sua ordem social à luz da formação do Sistema-Mundo. Os circuitos estendidos por Judeus Sefardis e Cristãos-novos envolvidos nas trocas de longadistância encontravam-se no bojo desse processo, desenvolvendo uma elite ultramarina capaz de traçar estratégias de mobilidade social através do patrocínio, matrimônios com nobrezas locais e outros meios. A trajetória de Ambrósio Fernandes Brandão é umexemplo desta atividade, permitindo revelar a agência envolvida em adquirir status o suficiente, dentre guerras e favores reais, para manter uma rede mercantil capaz de resistir a perseguição inquisitorial no Império Ultramarino ibérico.
Uma circunscrição judicial no Norte do Estado do Brasil. Juristas e juízes desenhando jurisdições (1654-1755)
Authors: Menezes Jeannie da Silva
Published: November 2020

A multiplicidade de atos de magistrados registrados nos mundos americanos vem atraindo o olhar da historiografia brasileira já há algum tempo, porém sob um prisma diferente da discussão contida neste artigo. Enquanto a historiografia brasileira se pauta pelas ações de juízes, entendendo o exercício jurisdicional como um limite para as múltiplas tarefas que a eles foram oferecidas ou por eles alargadas. Neste artigo, colocamos em cena os juristas etrazemos para a reflexão a ideia de que o desenho de circunscrições judiciais, de caráter administrativo, não separava magistrados de suas funções judiciais, porém materializava um pensar alargado sobre o exercício efetivo da justiça. Sobretudo porque tais funções resultaram também da interpretação do exercício jurisdicional descritopelos juristas para o mundo ibérico nos séculos XVII e XVIII. Entendemos que, naquele contexto de questionamentos sobre a extensão da soberania régia para além dos limites territoriais do reino, as contestações externas à monarquia portuguesa requisitaram a presença de letrados para “definir” alguns instrumentais nos domínios coloniais. Em especial, a criação de novas unidades administrativas e judiciais. Para caracterizar umfenômeno deste tipo, partimos de uma compreensão sobre o exercício jurisdicional que indicia a formação de uma nova composição formada pelas ouvidorias das Capitaniasdo Norte como um núcleo no qual as ações dos magistrados produziram o desenho de uma circunscrição judicial nos moldes daquelas que havia no reino à época.
Perancangan Aplikasi Penentuan Dosis Pemupukan Kelapa Sawit Menggunakan Metode SAW Berbasis Android
Authors: Ayu Fitri
Published: November 2020

Pemupukan merupakan salah satu syarat pemeliharaan tanaman termasuk pada tanaman kelapa sawit yang selama ini menjadi penghasil bahan baku utama untuk produksi minyak sawit. Namun sering terjadi persoalan-persoalan yang dialami para petani saat proses pemupukan seperti jenis pupuk yang tidak tepat dan dosis pupuk yang tidak sesuai dengan kebutuhan dilapangan sehingga para petani mendapatkan hasil panen yang tidak sebanding dengan jumlah biaya yang telah dikeluarkan untuk penggunaan pupuk. untuk mengatasi persoalan mengenai penentuan jumlah dosis pupuk diterapkan metode simple additive weightig (SAW) yang diimplementasikan dalam sistem pengambilan keputusan dalam menentukan dosis pemupukan pada tanaman kelapa sawit dengan menggunakan pupuk organik cair (POC) NASA. Proses pengolahan data yang telah didapat dari hasil pengujian sampel laboratorium oleh asosiasi Sawitku Masa Depanku (SAMADE) dianalisa dengan metode SAW untuk menentukan alternatif terbaik yang akan dijadikan sebagai acuan pada proses perhitungan terhadap rekomendasi dosis pupuk dengan cara membandingkan hasil konversi nilai setiap kriteria pada alternatif terpilih. Dari penelitian yang dilakukan dan telah diimplementasikan kedalam aplikasi yang dibangun berbasis android dapat memberikan hasil berupa rekomendasi jumlah dosis pupuk POC NASA terhadap tanaman kelapa sawit.
A New Interpretation of the Absorption and the Dual Fluorescence of Prodan in Solution
Authors: Vequi-Suplicy Cintia , Orozco-González Yoelvis , Lamy M. Teresa , Canuto Sylvio , Coutinho Kaline
Published: November 2020

Prodan is a fluorescent probe used to monitor biological systems such as lipid membrane,

proteins and DNA. Remarkable interest is associated to the interpretation of its fluorescent spectrum.

In this paper the sequential hybrid Quantum Mechanics/Molecular Mechanics (S-QM/MM) method was used to establish that the fluorescent emission occurs from two different excited states, resulting in a broad asymmetric emission spectrum with two transitions. The absorption spectra in several solvents were measured and calculated using different theoretical models and they all agree that the first observed band is composed of three electronic excitation very close energetically to each other.
Synthesis of γ-Oxo-α-amino Acids via Radical Acylation with Carboxylic Acids
Authors: Merkens Kay , Aguilar Troyano Francisco José , Anwar Khadijah , Gómez Suárez Adrián
Published: November 2020

Herein we present a highly efficient, light-mediated, deoxygenative protocol to access g-oxo-a-amino acid derivatives.This radical methodology employs photoredox catalysis, in combination with triphenylphosphine, to generate acyl radicals from readily available (hetero)aromatic and vinylic carboxylic acids. This approach allows for the straightforward synthesis of g-oxo-aamino acids bearing a wide range of functional groups (e.g. Cl, CN, furan, thiophene, Bpin) in synthetically useful yields (~ 60% average yield). To further highlight the utility of the methodology, several deprotection and derivatization reactions were carried out.
Pyclen-Based Ligands Bearing Pendant Picolinate Arms for Gadolinium Complexation
Authors: Nizou Gwladys , Molnár Enikő , Hamon Nadège , Kalman Ferenc K. , Fougere Olivier , Rousseaux Olivier , Esteban-Gómez David , Platas-Iglesias Carlos , Beyler Maryline , Tircso Gyula , Tripier Raphael
Published: November 2020

We report the synthesis of two pyclen-based regioisomer ligands (pyclen = 3,6,9,15-tetraazabicyclo[9.3.1]pentadeca1(15),11,13-triene) functionalized with picolinic acid pendant arms either at positions 3,9-pc2pa (L5) or 3,6-pc2pa (L6) of the macrocyclic fragment. The ligands were prepared by regiospecific protection of one of the amine nitrogen atom of the macrocycle using Boc and Alloc protecting groups, respectively. The X-ray structure of the Gd(III) complex of L5 contains trinuclear [(GdL5)3(H2O)3] 3+ entities in which the monomeric units are joined by 2- 1 : 1 carboxylate groups. However, the 1H and 89Y NMR spectra of its Y(III) analogue support the formation of monomeric complexes in solution. The Tb(III) complexes are highly luminescent, with emission quantum yields of up to 50% for [TbL5] + . The luminescence lifetimes recorded in H2O and D2O solutions indicate the presence of a water molecule coordinated to the metal ion, as also evidenced by the 1H relaxivities measured for the Gd(III) analogues. The Gd(III) complexes present very different exchange rates of the coordinated water molecule (kex 298 = 87.1 and 1.06 106 s -1 for [GdL5] + and [GdL6] + , respectively). The very high water exchange rate of [GdL5] + is associated to the steric hindrance originated by the coordination of the ligand around the water binding site, which favors a dissociatively activated water exchange process. The Gd(III) complexes present rather high thermodynamic stabilities (logKGdL = 20.47 and 19.77 for [GdL5] + and [GdL6] + , respectively). Furthermore, these complexes are remarkably inert with respect to their acid-assisted dissociation, in particular the complex of L5.
Crystallize Nanoparticles by Precipitating Trace Polymeric Additives
Authors: Qian Yiwen , da Silva Alessandra , Theis Wolfgang , Xu Ting , yu emmy , Anderson Christopher , Ercius Peter , Liu Yi
Published: November 2020

Growing nanoparticle (NP) crystals has been pursued extensively using ligand chemistries such as DNA and supramolecules, controlled evaporation and patterned surfaces. Here, we show that a trace amount of polymeric impurities (<0.1 wt.%) leads to reproducible, rapid growth of high quality 3-D NP crystals in solution and on patterned substrates with high yield. The polymers preferentially precipitate on the NP surfaces inducing the formation of small NP clusters, which subsequently act as nuclei to initiate NP crystal growth in dilute solution. This precipitation-induced NP crystallization process is applicable for a range of polymers and the resultant 3-D NP crystals can be tuned by varying polymeric additives loading, solvent evaporation rate and NP size. Fundamentally, the present study elucidates how to balance cohesive energy density and NP diffusivity in the self-assembly to favor nuclei formation energetically and kinetic growth in dilute solutions. The results shown also opened up the process window to rapidly and reliably fabricate NP crystals over multiple length scales. Furthermore, the amount of these impurities needed to grow NP crystals (<0.1 %) reminds us the need to pay special attention to fine details to interpret experimental observations in nanoscience.
Characterization of Protein Structure with Ion Mobility Mass Spectrometry, Multiplexed Fragmentation Strategies and Data Directed Analysis
Authors: Black Rachelle , Barkhanskiy Alexey , Ramakers Lennart , Theisen Alina , Brown Jeffery , Bellina Bruno , Trivedi Drupad , Barran Perdita
Published: November 2020

Activated ion mobility measurements provide Insights to the stability of tertiary and quaternary structures of proteins and pairing such approaches with fragmentation can delineate which part(s) of the primary sequence are disrupted from a folded structure. In this work we use 213 nm photodissociation coupled with ion mobility mass spectrometry and collisional activation to determine the conformational landscape of model proteins. UVPD experiments are performed on proteins following in source activation as well as on collisionally activated photoproducts post ion mobility separation. For cytochrome c, there is a significant increase in the fragmentation yield with collisional activation post mobility, for all conformational states. Similar strategies are deployed with the model multimeric proteins, concanavalin a, and haemoglobin. For these complexes’ CID leads to classic asymmetric charge distribution in subunit products, which when preceded by UV irradiation yields fragments from within the sub-unit that can be mapped to the quaternary fold. Data driven, multivariate analysis (MVA) was used to determine the significant differences in UVPD and CID fragmentation pattern following in source activation. This data driven approach reveals diagnostic fragments without a priori assignments limited to predicated backbone cleavage and provides a new approach to map conformation landscapes that may have wider utility.
Automated Fitting of Transition State Force Fields for Biomolecular Simulations
Authors: Wiest Olaf , Quinn Taylor R. , Patel Himani , Helquist Paul , Norrby Per-Ola , Koh Kevin J. , Haines Brandon E.
Published: November 2020

The application of the Quantum Guided Molecular Mechanics (Q2MM) method to transition states of enzymatic reactions to generate a transition state force field (TSFF) with the functional form of AMBER. The differences to fitting of small-molecule TSFFs and the similarities of the approach to transfer learning are discussed. The application to the transition state of the second hydride transfer in HMGCoA Reductase from Pseudomonas mevalonii is discussed.
Feshbach-Fano Approach for Calculation of Auger Decay Rates Using Equation-of-Motion Coupled-Cluster Wave Functions: Theory and Implementation
Authors: Skomorowski Wojciech , Krylov Anna
Published: November 2020

We present a novel methodology to calculate Auger decay rates based on equation-of -motion coupled-cluster singles and doubles (EOM-CCSD) wave function, combined with a simplified continuum orbital describing the outgoing electron. In our approach the Auger process is considered as an autoionization of a resonant electronic state, which can be described with Feshbach-Fano projection technique in order to distill the resonance parameters. To this end, we employ core-valence separation (CVS) scheme as a method to extract the bound part of the decaying many-electronic state. Main advantages of our methodology include (1) flexible EOM-CCSD ansatz enabling to describe various electronic states, (2) simple, yet universal computational setup, (3) fast computations due to fully analytical evaluation of all mixed bound-continuum two-electron integrals, and (4) implementation in general-purpose software package for quantum-chemical calculations.
Observation of an Intermediate to H2 Binding in a Metal–organic Framework
Authors: Barnett Brandon , evans hayden , Su Gregory M. , Jiang Henry Z. H. , Chakraborty Romit , Banyeretse Didier , Hartman Tyler , Martinez Madison , Trump Benjamin A. , Tarver Jacob , Dods Matthew , Drisdell Walter S. , Hurst Katherine , Gennett Thomas , FitzGerald Stephen , Brown Craig M. , Head-Gordon Martin , Long Jeffrey R.
Published: November 2020

Coordinatively-unsaturated metal sites within certain zeolites and metal–organic frameworks can strongly adsorb various molecules. While many classical examples involve electron-poor metal cations that interact with adsorbates largely through electrostatic interactions, unsaturated electron-rich metal centers housed within porous frameworks can often chemisorb guests amenable to redox activity or covalent bond formation. Despite the promise that materials bearing such sites hold in addressing myriad challenges in gas separations and storage, very few studies have directly interrogated mechanisms of chemisorption at open metal sites within porous frameworks. Here, we show that H2chemisorption at the trigonal pyramidal Cu+sites in the metal–organic framework CuI‑MFU-4l occurs via the intermediacy of a metastable physisorbed precursor species. In situpowder neutron diffraction experiments enable crystallographic characterization of this intermediate, the first time that this has been accomplished for any material. Support for a precursor intermediate is also afforded from temperature-programmed desorption and density functional theory calculations. The activation barrier separating the precursor species from the chemisorbed state is shown to correlate with a change in the Cu+coordination environment that enhances π-backbonding with H2. Ultimately, these findings demonstrate that adsorption at framework metal sites does not always follow a concerted pathway and underscore the importance of probing kinetics in the design of next-generation adsorbents.
Supramolecular Recognition in Crystalline Nanocavities Through Monte Carlo and Voronoi Network Algorithms
Authors: Schwalbe-Koda Daniel , Gomez-Bombarelli Rafael
Published: November 2020

Computational screening of templating molecules enables the discovery of new synthesis routes for zeolites. Despite decades of work in molecular modeling of organic structure-directing agents (OSDAs), the development and benchmarking of algorithms for docking molecules in nanoporous materials has received scarce attention. Here, we introduce Voronoi Organic-Inorganic Docker (VOID) a method based on Voronoi diagrams to dock molecules in crystalline materials, and release it as a Python package. Benchmarks of the implementation show it generates docked poses up to 95 times faster than the traditional Monte Carlo docking scheme. We then evaluate the algorithm by obtaining binding energies for about 120 zeolite-OSDA pairs of industrial relevance. The computed host-guest interactions explain experimental outcomes for traditional synthesis routes from the literature. The results further suggest new OSDAs to synthesize known zeolites. Finally, we exemplify the generality of VOID by docking molecules inside a metal-organic framework and on a metal surface. The proposed method and software provide a low-cost computational approach for generating molecule-material interfaces.
A Study of Aflatoxin on Sperm Cell Quality for Elderly People
Authors: Akila CR  , Dinesh Babu J  , Sravan Kumar P  , Vinaya B
Published: November 2020

Mycotoxins represent poisonous materials produced via certain types of growths. Among different mycotoxins, aflatoxins are viewed as plainly hazardous, taking into account that they are portrayed as cancer-causing for creatures and individuals. The admission of aflatoxins by means of feeds or nourishments should cause pernicious results on creatures' or people's wellbeing. Exploration on creatures has indicated that the overall casing circumstance just as a portion of the blood boundaries, particularly those of the liver may be adversely influenced with aflatoxin the executives. As to conceptive device, in spite of the fact that not remarkably examined, a few specialists bolster the helpless impacts of aflatoxins both on women or on grown-up guys. More precisely, in male, the scale and weight of the genital organs, the spermatogenesis, the number, the motility and the morphology of sperm cells just as hormones' fixations can be influenced after presentation of the creatures to aflatoxins, making barrenness inconveniences additional normal. Most examination allude to lab and significantly less to viable creatures, while least complex two investigations look for counsel from the doable issues of barrenness on men in view of aflatoxins. Since imitation is made one out of the greatest fundamental areas of creature cultivation, exceptional intrigue should be paid to supplements with the goal that the chance of the aflatoxin admission by creatures could be evacuated, the creature wellbeing extraordinarily in regards to the conceptive gadget may be covered and financial misfortunes could be enhanced.
Spread and Incubation Period of Coronavirus: A Review
Authors: Twinkle Francis  , Anjali AK
Published: November 2020

The Novel Corona Virus (2019 — n COV) has been causing an upsurge of pneumonia which is of viral origin that began in Wuhan, China. Covid-19 is a highly transmittable disease and a viral infection caused by a strain SARS-CoV-2 which has now spread around the world. COVID-19 contains a single-stranded (positive-sense) RNA associated with a nucleoprotein within a capsid containing matrix protein. The virus is a spherical, pleomorphic enveloped particle bearing club-shaped glycoprotein projections. Intermediate source of origin is not known, therefore, bats could be responsible as the primary reservoir. Transmission can also occur through families in their immediate environment around that particular infected person. Several healthcare workers and the patients who are admitted in the hospital are at higher risk of acquiring the infection. Most patients have a lack of access to Healthcare and preventive measures such as masks and sanitizers. This article discusses the various modes of spread and incubation period of the virus as well as approaches for containing the virus to cope with this viral outbreak.
Novel Sources of Drug Components in Plants
Authors: Sagar R  , Madhubhushan M  , Seshaiah S  , Chandrudu J
Published: November 2020

This survey is a refreshed and extended adaptation of a paper that was distributed in this diary in 1997. The time span has been reached out in the two headings to incorporate the 22 years from 1981 to 2002, and another optional development identified with the normal item source yet applied to officially engineered mixes has been presented, utilizing the idea of a "characteristic item copy" or "NM" to join the first essential separations. From the information introduced, the utility of characteristic items as wellsprings of novel structures, however not the last medication substance, is as yet perfectly healthy. In this way, in the region of malignant growth, the level of the little atom, new compound substances that are nonsynthetic has stayed at 62% found the median value of over the entire time. In different territories, the impact of characteristic item structures is very stamped, especially in the antihypertensive region, of which the 74 officially engineered drugs, 48 can be followed to standard item structures/copies. Correspondingly, with the 10 antimigraine drugs, seven depend on the serotonin atom or subsidiaries thereof.
Upcoming New Dimension in Drug Analysis Methods
Authors: Priyanka G  , Sravani G  , Linga Naik A  , Kranthi A
Published: November 2020

In recent decades, the scientific procedures relating to medicate location and portrayal have advanced an incredible arrangement bringing about the turn of events, improvement and approval of huge swath of strategies and methods. Proceeded with endeavours are being made for polluting influence profiling while at the same time acquiring better exactness and accuracy for detachment and evaluation of the analytes. Pharmaceutica Analytica Acta reports these advances intermittently. The current issue of the diary centres around conveyance of nasal splash bead circulation, in silico concentrates on Spebrutinib and pharmaceutical examination dependent on stream investigation strategies.
Preparation and Analysis of Coumarin Compound for New Application
Authors: Sravani G  , Linga Naik A  , Kranthi A  , Priyanka G
Published: November 2020

Numerous normally occurring substances usually applied in famous drugs the world over, incorporate the coumarin moiety. Coumarin speaks to a unique platform for restorative scientists, in light of its unconventional physicochemical highlights, and the adaptable and easy engineered change right into a huge assortment of functionalized coumarone. As an outcome, countless coumarin subordinates were dependent, incorporated, and tried to cope with numerous pharmacological focuses in a particular way, e.G., specific chemical inhibitors, and all of the extra as of past due, numerous chose targets (multitarget ligands) associated with multifactorial illnesses, as an instance, Parkinson's and Alzheimer's infections. In this survey, a definition of today's manufactured paths prompting mono-and polyfunctionalized coumarins might be introduced, alongside the precept natural pathways in their biosynthesis and metabolic adjustments. The numerous current and overdue audits in the discipline provoked us to type-specific terrific alternatives. Subsequently, the survey is targeted around following up on chosen focuses on neurodegenerative illnesses.