bluuebunny/crossref_metadata_2025 huggingface dataset:
Tolerancia de híbridos del género Brachiaria a diversas condiciones salinas de invernadero en Santa Elena, Oaxaca
Authors: Sánchez-Bernal Edgar Iván , Ortega-Escobar Héctor Manuel , Ortega-Baranda Verónica , Can-Chulim Álvaro , López-Garrido Serafín Jacobo , Ochoa-Somuano Jorge
Published: June 2023

En un invernadero de la costa de Oaxaca, se evaluó la tolerancia de los pastos híbridos del género Brachiaria: Cobra (BR02/1794), Cayman (BR02/1752), Camello (GP 3025) y Mulato II (CIAT 36087) a condiciones salinas inducidas por NaCl, MgCl2 y NaHCO3 y siete niveles de salinidad: 0, 1.13, 2.48, 3.84, 5.19, 6.40 y 9.60 g L-1. Se registró el porcentaje de emergencia, altura de plantas, diámetro de tallo, número de hojas y macollos, longitud de raíz y producción de biomasa. El diseño experimental fue factorial con tres sales, siete niveles salinos, cuatro pastos y tres repeticiones, con 252 unidades experimentales. El porcentaje de emergencia aumentó con el tiempo en todos los casos y parámetros: altura de plantas, diámetro de tallo, número de hojas y macollos, longitud de la raíz y producción de biomasa total disminuyeron al incrementar el nivel salino en el siguiente orden: NaCl < MgCl < NaHCO3. Los datos indican que el pasto que produjo más biomasa (2.23 g pl-1) fue Cobra a un umbral salino de 5.19 g L-1 de NaCl, por lo que se considera de moderada sensibilidad; el pasto que produjo la menor biomasa (0.34 g pl-1) fue Cayman a una concentración de 1.13 g L-1 de NaHCO3, categorizándolo como muy sensible. Las tres sales experimentales a concentraciones > 6.40 g L-1 produjeron plantas enanas e inhibición del crecimiento, fenómeno intensificado por NaHCO3. La baja tolerancia de los pastos a las sales NaCl, MgCl2 y NaHCO3 a concentraciones > 3.84 g L-1 limita su cultivo en la llanura costera de Oaxaca. Sin embargo, su implementación en campo es posible con la aplicación de técnicas sustentables de uso y manejo del suelo y del agua de riego, para aminorar los efectos de la salinidad y mejorar la producción de forraje.
Design of Web-Based Management Information System for Student Organizations in Kendal Regency Using Next.js Framework
Authors: Susanto Fx Guntur Putra , Fadlan Nizar Izzuddin Yatim , Haryani Prita
Published: June 2023

Regional student organizations are essential for student development and fostering regional peer relationships. However, regional student organizations faced challenges in management due to the lack of digitization such as in the approval of proposals, submission of work programs, and accountability reports. With these challenges, the development of information systems was essential for simplifying regional student organization management and increasing member participation and involvement. This research focused on the information system design stage, which includes database design and interface design where the goal is to design an information system for Kendal area student organizations using Node.js technology and Next.js frameworks that use the System Development Life Cycle with a waterfall model for the research method. Next.js is a popular framework that simplified web application development with server-side rendering and static site generation, enhancing system performance and reliability. In conclusion, the design of a Web-Based Management Information System for Student Organizations in Kendal Regency using the Next.js framework successfully incorporated various system components, including Unified Modelling Language diagrams, Entity-Relationship Diagrams, system architecture, and user interface design. Future research should focus on the development and implementation of the proposed system to further improve the management of student organizations in Kendal District.
Decision-Making Analysis Of Koi Pool Pump Selection Using Analytical Hierarchy Process (AHP) Method
Authors: Astuti Marni , Ibrahim Ramadhan Rafiq , Zabidi Yasrin , Nurdin Riani , Mauidzoh Uyuunul
Published: June 2023

The availability of various koi pond pump products is marketed with various kinds, types, and various advantages. This diversity makes it difficult for business owners to determine a koi pond pump product that suits their business needs. The consideration of the pump in question is a koi pond pump in the form of an electric submersible pump or ESP. One method that can be used for the selection of a koi pond pump is the AHP (Analytical Hierarchy Process) method. This study uses the AHP method assisted by expert choice software. From the results of the assessment of the importance of the criteria in the selection of suppliers, the priority scales are as follows: Priority I Brand Reputation (0.4251), Product Design (0.2661), Power Consumption (0.1102), Durability (0.1074), Ease of Maintenance (0.054), and Price (0.0409). From the results of the alternative assessment, the following priorities were generated: priority I Eheim CON12000 (0.4105), Resun PG12000 (0.2318), Yamano ACT12000 (0.2025), and Atman MP12000 (0.1551).
Educational collections of the Commission for the Geological Map of Spain in schools and in the Royal National Heritage Collections
Authors: Rábano, Isabel
Published: June 2023

The aid provided to schools by the Commission for the Geological Map of Spain, and its heir institutions -the Geological Institute of Spain and the Geological and Mining Institute of Spain- from the last third of the nineteenth century is presented. A Royal Decree published on February 12, 1888 entrusted the Commission to send geological collections to educational centers for the support of geological and mining teaching. The absence of a historical archive in the Geological and Mining Institute of Spain prevented us from knowing the scope of this cooperation, which can be considered as a pioneer activity of the Institute in the dissemination of Earth Sciences to society. Through intensive research work in which various sources have been managed, it has been possible to identify some of these collections. Not all of them are preserved in their entirety, and in some cases only the catalog that accompanied the samples is known. This research has allowed us to know the patterns of formation of the collections, as well as the authorship of some of them. It has also studied the one that was sent in 1902 to the King Alfonso XIII, which is currently preserved in the Royal National Heritage Collections. Among the specimens in this collection, one of the lost types of the Cretaceous rudist Hippuritella castroi (Vidal, 1874) has been identified. Se presentan las evidencias actuales de la ayuda prestada por la Comisión del Mapa Geológico de España, y sus instituciones sucesoras -el Instituto Geológico de España y el Instituto Geológico y Minero de España-, a los centros educativos a partir del último tercio del siglo XIX. Un Real Decreto publicado el 12 de febrero de 1888 encomendó a esta Comisión la remisión de colecciones geológicas a establecimientos de enseñanza para el apoyo de las enseñanzas geológicas y mineras. La ausencia de archivo histórico en el Instituto Geológico y Minero de España impedía conocer el alcance de esta cooperación, que se puede considerar una actividad pionera en la institución en relación con la difusión de las Ciencias de la Tierra a la sociedad. Mediante una intensa labor de investigación en la que se han manejado diversas fuentes, ha sido posible identificar algunas de estas colecciones. Y las que se conservan no suelen estar completas: en algunos casos solo se ha podido consultar el catálogo que acompañaba a las muestras. Pero se ha llegado a conocer las pautas de formación de las colecciones, así como la autoría de algunas de ellas. Se ha estudiado también la que fue remitida en 1902 al rey Alfonso XIII, que se conserva en las Colecciones Reales del Patrimonio Nacional. En ella se ha identificado uno de los tipos perdidos del rudista cretácico Hippuritella castroi (Vidal, 1874)
The Sinemurian (Lower Jurassic) Gastropod Collection from the “Comissões Geológicas” of Portugal
Authors: Vitón Í. , Comas-Rengifo M.J. , Paredes R.
Published: June 2023

The “Comissões Geológicas” of Portugal evolved from 1857 to 1918. Paul Choffat, who specialised in the study of the Mesozoic stratigraphy and palaeontology, was one of its members and an active collaborator. He and his collectors compiled a reference extensive collection of Sinemurian gastropods, mainly collected from the S. Pedro de Moel and Coimbra areas. A total of 395 specimens are currently recognisable; the remaining ones were lost in fires or relocations. The major part of the present collection (74.68%) is housed in the Museu Geológico (Lisbon), where the labels are preserved and where the type and figured specimens in classical works are kept; the remaining specimens can be found in three other institutions, where they mostly resulted from offers: the Museu Nacional de História Natural e da Ciência – Universidade de Lisboa, the Museu da Ciência da Universidade de Coimbra, and the Museu de História Natural e da Ciência da Universidade do Porto. These collections have unique taxonomical and stratigraphical information about outcrops no longer accessible. The creation of a virtual support not only makes the universal access easier, but also contributes to a better preservation of this invaluable material Las “Comissões Geológicas” se desarrollaron en Portugal entre los años 1857 y 1918. Paul Choffat, quien se especializó en el estudio de la paleontología y estratigrafía del Mesozoico, fue uno de sus miembros y colaboradores activos. La colección de gasterópodos del Sinemuriense que Choffat y sus colaboradores recolectaron proviene mayoritariamente de S. Pedro de Moel y de los alrededores de Coimbra. En la revisión realizada se han encontrado un total de 395 ejemplares, aunque debido a los sucesivos incendios y traslados, la cifra original seguramente fuera mayor. La mayor parte de la colección actual (74,68 %) se encuentra en el Museo Geológico (Lisboa), donde se conservan las etiquetas originales y donde están guardados los tipos y ejemplares figurados en los trabajos clásicos; los ejemplares restantes, provenientes principalmente de cesiones, se encuentran distribuidos en otras tres instituciones: el Museo Nacional de Historia Natural y de la Ciencia (Lisboa), el Museo de Ciencia de la Universidad de Coimbra (Coimbra), y el Museo de Historia Natural y de la Ciencia de la Universidad de Porto (Oporto). Todas estas colecciones tienen información taxonómica y estratigráfica única de afloramientos que ya no son accesibles. La creación de una plataforma virtual no solo haría más fácil el acceso universal a esta información, sino que contribuiría a una mejor conservación de este material de incalculable valor.
Ectopic teeth in the maxillary sinus
Authors: Melnichenko Y. M. , Kabak S. L. , Savrasova N. A. , Mekhtiev R. S.
Published: June 2023

Dental ectopia is a rare developing anomaly characterized by a change in the normal eruption pathway of a deciduous, permanent or supernumerary tooth. In some cases, ectopic teeth are present in the non-dentate area like maxillary sinus. The article presents cone beam computed tomography data of 7 asymptomatic patients with teeth totally or partially located inside the maxillary sinus. Dentigerous cysts and odontomas can cause displacement of impacted teeth into ectopic positions.
PCR with hydrolysis probes for detection of fluoroquinolone resistance mutations in Mycobacterium tuberculosis
Authors: Slizen V. V. , Surkova L. K. , Gurevich G. L.
Published: June 2023

Early diagnostics of resistance to fluoroquinolones facilitates early start of adequate therapy and increases the chance of a favorable outcome of the tuberculosis. Application of genetic methods permits to obtain within 1–2 days the results of Mycobacterium tuberculosis resistance detection to anti-tuberculosis drugs, unlike the classical methods requiring up to 1−2 month.

The aim of the study is to develop a method for the M. tuberculosis identification and detection of point mutations in codons 90, 91, 94 of the gyrA gene associated with the resistance to fluoroquinolones.

There were 88 cultures of mycobacteria studied: M. tuberculosis (n = 81), M. tuberculosis H37Rv, M. chelonae (n = 1), M. gordonae (n = 1), M. fortuitum (n = 1), and other three isolates of non-tuberculosis mycobacteria isolated from patients in the Republican Scientific and Practical Center for Pulmonology and Phthisiatry. The types of mutations in the gyrA gene were studied by the standard GenoTypeMTBDRsl method (HAIN, Germany), Sanger sequencing, and by the developed real-time PCR method. Based on the analysis of mutations in the gyrA gene in 78 isolates of M. tuberculosis, the dominant mutations were found to be mutations Asp94Gly and Ala90Val, which were identified in 21 and 27 isolates correspondingly: they accounted for 64 % of all mutations. M. tuberculosis also harbored mutations p.ASP94ALA and p.ASP94TYR/HIS in 6 (8.0 %) and 9 (12.0 %) isolates, respectively. One strain harbored a mutation at triplet 88 and one strain had a double mutation (p.ALA90VAL and p.ASP94GLY). The developed real-time PCR method demonstrated a high frequency of coincidence of results with the phenotypic determination of resistance to ofloxacin and the results testing by the standard GenoTypeMTBDRsl method and sequencing.

The developed method is accomplished to identify M. tuberculosis, and discriminate mutations p.ALA90VAL, p.SER91PRO, p.ASP94ALA, p.ASP94TYR/HIS, p.ASP94GLY, p.ASP94ASN providing diagnostics of resistance to fluoroquinolones.
The main directions for increasing the efficiency of the use of reclaimed lands in modern conditions
Authors: Lopatnyuk A. A. , Tivo P. F. , Lopatnyuk L. A.
Published: June 2023

The main problems associated with the use of reclaimed lands are considered. The influence of technological and economic techniques and methods on the level of rational use of drained soils is substantiated.

The value of the drawdown of the upper organic layer on the agrochemical properties of drained lands has been determined. The costs of restoring the lost soil fertility are calculated. The main directions for increasing the efficiency of crop cultivation on reclaimed lands are proposed.
Back Matter
Authors: Bengen Dietriech Geoffrey
Published: June 2023

Back Matter
NUTRITIONAL VALUE AND HEAVY METAL CONTENT OF CRAB MEAT AND ITS BYPRODUCT WHITE MUD CRAB Scylla paramamosain
Authors: Ardiansyah Ardi , Nugroho Aji , Meirinawati Hanny
Published: June 2023

Mud crab is fishery commodity that has high economic value and rich of nutritional aspect such as essential amino acids, vitamins, minerals, and fatty acids. In addition, high concentrations of heavy metals in the natural habitat of crabs can accumulate in these organisms which can endanger health if consumed. However, there is little information regarding the nutritional and heavy metal content of meat and byproducts in mud crab. The objective of the study are to compare the beneficial component of the meat and byproduct of Scylla paramamosain and to investigate food safety from heavy metal contamination. Proximate, vitamin A, D, and E content were analyzed using HPLC, vitamin B1, B2, C, and amino acid profile was determined by using UPLC, vitamin B12 was determined by LC-MS, mineral and heavy metal were determined by standard method of AOAC. In addition, the determination of fatty acid was analyzed by gas chromatography. Result showed that protein per gram as the majority content of each form (10.24% in crab meat and 12.47% in byproduct). Vitamin B2, B12, and E were detected in sample. On contrary, heavy metals examined in this study were not detected. L-Glutamic acid was the most abundant of amino acids composition (11037.79 mg/kg of crab meat and 15993.22 mg/kg of byproduct), while omega-3 fatty acids, omega-6 fatty acids, and omega-9 fatty acids are also contained in this species. The result showed Scylla paramamosain as source of beneficial food and safe to consume.
PENGARUH PERBEDAAN pH PERAIRAN TERHADAP LAJU PERTUMBUHAN LAMUN JENIS Cymodocea rotundata
Authors: Yudho Andika  , Kawaroe Mujizat , Effendi Hefni , Zamani Neviaty Putri , Erniati  , Erlangga  , Adhar Saiful , Imanullah  , Imamshadiqin  , 'Akla Cut Meurah Nurul , Sugara Ayub , Ilhami Bq Tri Khairani
Published: June 2023

Penggunaan bahan bakar fosil yang terus berlangsung akan meningkatkan konsentrasi karbondioksida (CO2) di atmosfer. Asidifikasi laut terjadi akibat CO2 yang berada di atmosfer berdifusi ke lautan. Lautan mampu menyerap CO2 di atmosfer sebanyak 35 % lebih yang menyebabkan terjadinya penurunan pH laut. Lamun Cymodocea rotundata merupakan salah satu jenis lamun yang banyak ditemukan tumbuh di perairan tropis. Keadaan ini menimbulkan kekhawatiran tentang kemungkinan terjadinya dampak pada pertumbuhan lamun C. rotundata. Penelitian ini bertujuan untuk menganalisis kandungan nitrat, fosfat dan kalium dan pertumbuhan lamun C. rotundata yang meliputi pertumbuhan daun, rhizoma, dan akar C. rotundata terhadap perbedaan pH. Penelitian menggunakan metode eksperimen dengan rancangan acak lengkap menggunakan tabel acak. Sebanycak 15 toples dengan ukuran diameter 20 cm dan tinggi 25 cm digunakan dengan 3 perlakuan, masing-masing perlakuan 5 kali pengulangan. Hasil uji regresi linier menunjukkan pH berpengaruh terhadap konsentrasi nitrat, dan berpengaruh kuat terhadap konsentrasi fosfat dan kalium. Laju pertumbuhan daun lamun C. rotundata tertinggi pada kontrol berkisar antara 0,50–1,29 mm/hari sedangkan yang terendah pada pH rendah berikisar 0,07–0,73 mm/hari. Laju pertumbuhan rizhoma lamun secara horizontal dan vertikal tertinggi pada pH rendah sedangkan yang terendah pada pH kontrol. Laju pertumbuhan akar lamun tertinggi pada pH rendah berkisar antara 0,20–0,90 mm/hari. sedangkan yang terendah pada kontrol berkisar antara 0,13–0,43 mm/hari. pH juga memengaruhi laju pertumbuhan daun, rhizoma dan akar lamun C. rotundata. Semakin rendah pH maka laju pertumbuhan daun juga semakin rendah, berbeda dengan rhizoma dan akar semakin rendah pH maka semakin tinggi laju pertumbuhan.
PEMETAAN KERENTANAN WILAYAH PESISIR TERHADAP KENAIKAN MUKA AIR LAUT DI KABUPATEN LEBAK BANTEN
Authors: Oktaviani Laela , Rahmawati Ani , Muta Ali Khalifa
Published: June 2023

Daerah pantai yang dinamis memberikan respons terhadap peristiwa ekstrem salah satunya adalah kenaikan muka air laut. Hal tersebut akan memberikan dampak buruk pada kondisi wilayah pesisir, seperti pada Pantai Binuangeun yang sekarang ini mengalami abrasi akibat adanya hantaman gelombang besar. Hal ini menunjukkan wilayah pesisir Lebak berpotensi mengalami kerentanan, terutama akibat kenaikan muka air laut. Penelitian ini bertujuan untuk memetakan sebaran kerentanan wilayah Pesisir Kabupaten Lebak Banten terhadap kenaikan muka air laut. Metode yang digunakan dalam penelitian ini adalah Coastal Vulnerability Index (CVI). Data yang dikumpulkan terdiri dari data primer yang diperoleh dengan melakukan observasi lapangan dan data sekunder yang diperoleh melalui NOAA, Copernicus, dan IOC Sea Level Monitoring. Variabel kerentanan yang dianalisis adalah geomorfologi, perubahan garis pantai, elevasi, kenaikan muka air laut, tunggang pasang surut, dan tinggi gelombang. Karakteristik pantai Lebak adalah karang, berpasir, dan tebing. Penilaian kerentanan dibagi menjadi 3 kelas, yaitu rendah, sedang, dan tinggi. Hasil penelitian menunjukkan bahwa nilai Coastal Vulnerability Index (CVI) pesisir Lebak berkisar antara 2,9-2,19. Nilai tersebut menunjukkan seluruh kecamatan pesisir Kabupaten Lebak termasuk dalam kategori kerentanan rendah terhadap kenaikan muka air laut. Oleh karenanya pesisir Lebak tidak berpotensi terdampak bencana-bencana yang diakibatkan oleh kenaikan muka air laut.
HEALTH BPJS PUBLIC SERVICE MODEL TO REALIZE GOOD GOVERNANCE IN INDONESIA
Authors: Fardillah Roni , Widijowati RR. Dijan , Ismail Ismail
Published: June 2023

Pelaksanaan good governance dapat dijadikan sebagai bagian dari upaya untuk melaksanakan asas-asas demokrasi dan demokratisasi yang merefleksikan dijunjung tingginya aspek pemenuhan hak-hak rakyat oleh penguasa, ditegakkan nya nilai-nilai keadilan dan solidaritas sosial, serta adanya penegakan hak asasi manusia (HAM) dalam berbagai aspek kehidupan negara. Penelitian ini bertujuan untuk mengetahui dan memahami permasalahan mengenai bagaimana pelaksanaan prinsip-prinsip good governance dalam melayani BPJS kesehatan di indonesia salah satu masalah yang terjadi yaitu kurang nya responsif tenaga medis terhadap pasien BPJS di indonesia penelitian ini berusaha untuk mengungkap masalah yang berhubungan dengan prinsip-prinsip good governance dengan menggambarkan suatu fakta yang berkaitan dengan BPJS kesehatan di indonesia  dan alasan penulis meneliti secara nasional karena hampir semua pasien BPJS kesehatan di daerah yang ada di Indonesia memiliki keluhan yang sama terkait masalah  pelayanan pelayan pasien BPJS kesehatan di Indonesia.
ABORDAGENS HOLÍSTICAS NA PEDIATRIA: CONSIDERANDO O BEM-ESTAR FÍSICO, EMOCIONAL E SOCIAL DA CRIANÇA
Authors: Tuyama Marina Gontijo , Cabral Maria Eduarda Galhardo , MELO VITÓRIA GUERRA , MALFERA FABIANA BUCHEMI CARDOSO , Simião Caroline Marinho , FREITAS GABRIELA PASCHOALIM , Viana Victoria Vecchi Pacheco , Evangelista Junia Murta Pedras Lopes , Salles Ana Bárbara Dumont , AZEVEDO LETÍCIA MAIA
Published: June 2023

As abordagens holísticas na pediatria têm se mostrado essenciais para promover o bem-estar físico, emocional e social da criança. Essas abordagens reconhecem a importância de tratar a criança como um todo, considerando não apenas seus sintomas físicos, mas também aspectos emocionais e sociais. Este artigo explorou os benefícios das abordagens holísticas na pediatria, destacando sua integração com a medicina convencional, o impacto positivo no bem-estar físico, emocional e social da criança, a promoção de hábitos saudáveis e a importância do acesso a serviços de saúde ginecológica. Além disso, foram abordados os desafios e perspectivas futuras nessa área. Ao adotar uma abordagem holística, os profissionais de saúde podem fornecer cuidados abrangentes e personalizados, melhorando a qualidade de vida e o desenvolvimento saudável das crianças. A integração adequada entre medicina convencional e abordagens holísticas é fundamental para garantir uma abordagem segura e baseada em evidências. Em suma, as abordagens holísticas na pediatria desempenham um papel crucial na promoção do bem-estar global da criança e devem ser consideradas como parte integrante do cuidado infantil.
Palavras Chave: Abordagens holísticas. Bem-estar infantil. Pediatria integrativa.
Toxicidade dos Biocidas Irgarol e Diuron em Água Salobra e Salina para Vibrio fischeri e de Embriões de Crassostrea rhizophorae
Authors: Matos Lisandra Maria Barroso , Brito Daniel Richard Lemos , Correa Jacyara Nascimento , Torres Thamires Alexandra , Jorge Marianna Basso , Santos Ricardo Luvizotto
Published: June 2023

Tintas anti-incrustantes são aplicadas como sistemas de proteção em superfícies submersas ou semi-submersas a fim de combater o estabelecimento da bioincrustação. Esses biocidas utilizados possuem elevada toxicidade e certa persistência no ambiente, podendo afetar organismos não-alvo. Os biocidas aplicados em sistemas anti-incrustantes têm sido detectados em amostras ambientais em diferentes localidades e estudos recentes identificaram a presença de irgarol e diuron no Complexo Estuarino de São Marcos (CESM - São Luís, MA) em níveis que podem representar risco ao ecossistma. O objetivo deste estudo foi determinar a toxicidade aguda dos biocidas irgarol e diuron em água salina e salobra para a bactéria bioluminescente Vibrio fischeri e para embriões/larvas da ostra-do-mangue Crassostrea rhizophorae, espécie representante da fauna local. Com base nos valores de CE50(30min) para V. fischeri e CE50(24h) para C. rhizophorae, o irgarol foi o biocida que apresentou maior toxicidade. Além disso, a toxicidade do irgarol foi maior na água salobra (25g/kg), sugerindo maior risco às comunidades estuarinas no período das chuvas, quando há predomínio das águas estuarinas no CESM.
Contaminação de peixes pelos compostos organofosforados e a resposta da Citocromo oxidase P450 (CYP450): revisão da literatura e vias metabólicas ativadas pelo transcriptoma no fígado de tambaqui
Authors: Carvalho Ana Paula Costa , Silva Hallana Cristina Menezes da , Gomes Ana Lúcia Silva , Artoni Roberto Ferreira , Matoso Daniele Aparecida
Published: June 2023

No Brasil existem diversos produtos antiparasitários aprovados para uso na aquicultura. O triclorfon é um composto organofosforado com efeito genotóxico e amplamente utilizado para controlar uma variedade de ectoparasitoses na piscicultura. Em contato com a água sofre hidrólise e forma um metabólito potencialmente mais tóxico, o diclorvós. A biotransformação pode reduzir a meia-vida do xenobiótico diminuindo seu potencial tóxico. As enzimas da superfamília citocromo P450 (CYP) fazem parte da desintoxicação de muitos agroquímicos em peixes e são importantes na biossíntese e metabolismo de compostos endógenos. As respostas mensuradas ao nível de gene, assegura a aplicação destes biomarcadores moleculares como ferramentas de monitoramento para avaliar o impacto dos contaminantes no meio ambiente. Desse modo, o presente estudo realizou uma revisão de literatura para entender sobre os mecanismos e respostas da CYP450 em peixes contaminados e suas principais vias metabólicas.
Padronização e aplicação do Ensaio Cometa no peixe Centropomus undecimalis: uma análise da genotoxicidade de ambientes estuarinos
Authors: Cantanhêde Sildiane Martins , Castro Geane da Silva , Sousa Denise da Silva , Tchaicka Lígia , Santos Débora Martins Silva
Published: June 2023

Esta pesquisa adaptou a técnica do Ensaio Cometa na espécie Centropomus undecimalis, aplicando-a na avaliação da genotoxicidade de dois ambientes estuarinos na Ilha do Maranhão, rio Bacanga e estuário do Pau Deitado. Os peixes foram coletados no período chuvoso de 2015 e o sangue foi retirado por punção branquial para a padronização. Os parâmetros físico-químicos da água foram mensurados no local. Para a avaliação dos danos do DNA, os nucleoides foram classificados em classes de 0 a 3, segundo o grau de danos. A padronização da técnica teve alguns desafios, sendo o principal a fixação da agarose após o banho na solução Lise. Com isso, o tempo de exposição na solução foi alterado, bem como o tempo de corrida eletroforética. Os parâmetros físico-químicos da água dos pontos amostrados apresentaram valores dentro do permito pela legislação nacional, exceto a concentração de oxigênio dissolvido em ambos os pontos. Foram observados danos de todas as classes no DNA de C. undecimalis de ambos os pontos, os peixes do rio Bacanga apresentaram maior média de DNA com nível alto de danos (Classe 3) e os peixes do estuário do Pau Deitado apresentaram maior média de DNA com nível médio de danos (Classe 2). O valor médio do Score de danos no DNA foi maior nos peixes do Rio Bacanga, indicando que esses animais estão expostos a substâncias genotóxicas que induzem alterações e lesões no DNA. A técnica do Ensaio Cometa em C. undecimalis foi adaptada com poucas alterações e os danos no DNA detectados pela técnica mostraram-se como metodologias eficazes na avaliação da qualidade ambiental dos estuários amostrados. Dessa forma, a metodologia padronizada pode subsidiar programas de biomonitoramento nas áreas. Além disso, é necessário expandir a diversidade de organismos estudados por essa técnica na região, para serem usados em estudos de biomonitoramento.
Avaliação de impactos ambientais na região industrial e portuária de São Luís utilizando Sciades herzbergii (Siluriformes: Ariidae) como biomonitor: uma revisão
Authors: Macedo Gustavo Henrique Rodrigues Vale de , Costa Ana Laíssa Pereira , Ribeiro Rafael do Carmo Silva , Pires Samanda de Jesus Roland , Neulls Talita Fernandes , Toledo Carlos Augusto Barboza , Fontenelle Pedro Henrique Cunha , Castro Jonatas da Silva , Neta Raimunda Nonata Fortes Carvalho , Sousa Débora Batista Pinheiro
Published: June 2023

Regiões industriais/portuárias são grandes responsáveis pela descarga de substâncias tóxicas que afetam diretamente organismos aquáticos residentes e, consequentemente, a saúde humana. Esta revisão busca avaliar como biomarcadores presentes em Sciades herzbergii podem ser utilizados para avaliação de impactos ambientais na região industrial e portuária de São Luís-MA. Os resultados mostraram que a utilização de peixes como bioindicadores tem sido amplamente explorada, pois as intensas atividades antrópicas em áreas industriais e portuárias favorecem a exposição de espécies residentes a xenobióticos químicos – como hidrocarbonetos policíclicos aromáticos (HPAs), grupos de elementos-traço, organo estanhos, organoclorados e compostos fenólicos –, o que aumenta a expressão de uma variedade de marcadores biológicos. Como um dos principais organismos biomonitores, Sciades herzbergii se destaca por ser um espécie não-migratória amplamente difundida e com grande tolerância ambiental, bem como alta atividade de bioacumulação tecidual de xenobióticos. Estudos ecotoxicológicos mostram que em S. herzbergii podem ser observados diversas alterações, como índices biométricos, lesões teciduais, aumento da expressão de enzimas e da atividade bioquímica, além de variações na microbiota natural da pele e de órgãos internos. Assim, estudos que avaliem a qualidade da água dos estuários e a sanidade de S. herzbergii residentes no complexo portuário de São Luís-MA podem ser extremamente úteis para o estabelecimento de estratégias e políticas de monitoramento ambiental, saúde pública e conservação em áreas passíveis de contaminação química, contribuindo para a elaboração de planos de manejo da região.
ESTADÍSTICA ANUAL DE ACCIDENTES DE ORIGEN ELÉCTRICO 2023 - Año base 2022
Authors: Souza Danilo Ferreira de , Martinho Edson , Martinho Meire Biudes , Martins Jr. Walter Aguiar
Published: June 2023

Tres criterios básicos guían la estructura del Anuario Estadístico de Accidentes de Origen Eléctrico 2022: 1) accidentes con descargas eléctricas, 2) accidentes con incendios por sobrecarga de las instalaciones eléctricas y 3) accidentes con descargas atmosféricas. Los datos se recogieron entre el 1 de enero y el 31 de diciembre de 2022. La información se organiza en seis secciones. La primera sección presenta el número total de accidentes - con y sin muertes - resultantes de accidentes de origen eléctrico en Brasil: descargas eléctricas, incendios por sobrecarga de energía (cortocircuito) y caída de rayos. La segunda sección presenta datos sobre descargas eléctricas, con recortes regionales y el tipo de edificio o lugar donde se produjeron los accidentes. Las estadísticas también indican el grupo de edad y el tipo de ocupación de los heridos. Del mismo modo, la tercera sección ofrece información sobre incendios debidos a sobrecargas o cortocircuitos. La cuarta sección presenta datos más amplios y generales sobre los accidentes por rayos. Todos estos datos confirman el escenario de las instalaciones eléctricas en Brasil. En la cuarta sección, ofrecemos datos que compilan las ocurrencias de accidentes desde el inicio de la computación de datos por Abracopel. En la quinta sección, presentamos la serie histórica. Los datos destacan los accidentes con descargas eléctricas, incendios por sobrecargas de energía y descargas atmosféricas (rayos) en nueve años (2013-2022). De este modo, es posible establecer comparaciones y perspectivas de aumento o disminución de los accidentes. Esta edición también incluye una sección especial sobre la calidad de los hilos y cables. En la sexta sección, están las consideraciones finales del documento con una breve comparación de datos a nivel mundial, un plan de acción simplificado para orientar las acciones de Abracopel y algunas noticias divulgadas en los periódicos.
ENSINO DE QUÍMICA EM REVISTA - VOLUME 6
Authors: Souza Adriana Baptista de , Thomé Amanda Ramos de Mattos , Guerra Antonio Carlos de Oliveira , Rocha Angela Sanches , Silva Carla Márcia Alvarenga da , Silva Célia Regina Sousa da , Silva Fernanda Arruda Nogueira Gomes da , Rosa Flávio Matheus , Silva Joaquim Fernando Mendes da , Camargo Joyce Braga , Silva Lohrene de Lima da , Tamiasso-Martinhon Priscila , Almeida Rodrigo Volcan , Nunes Valeria Fernandes , Teixeira Viviane Gomes
Published: June 2023

Organizada desde 2016, Ensino de Química em Revista é uma edição do Programa de Pós-Graduação em Ensino de Química da UFRJ (PEQui – IQ – UFRJ). São reunidos aqui textos de egressos do Programa e manuscritos que atenderam ao edital específico para este sexto volume, revistos às cegas por pares do Comitê Científico Editorial. Ensino de Química em Revista está ciente do seu papel de veículo para discussões que se dedicam aos aspectos plurais da formação de professores de química. Adotamos como princípio que esta divulgação deve enriquecer o olhar do outro pelo compartilhamento da produção de pesquisadoras e pesquisadores, já bem estabelecidos ou em formação.

Desejamos a todas e todos excelente leitura e reflexão.

Os organizadores
Probing nuclear equation of state with the cdm3y version of B3y-fetal effective interaction
Authors: Ochala Isaiah
Published: June 2023

This paper is a study of the nuclear Equation of State (EOS) of cold nuclear matter with the B3Y-Fetal effective interaction in its CDM3Y density-dependent version within the framework of Hartree-Fock approximation. The well-known saturation properties of both symmetric and asymmetric nuclear matter are well-reproduced in this work. Using the CDM3Y-K approach, this study has evolved a new set of user interactions, some of which are CDB3Y1-, CDB3Y2-, CDB3Y3-, CDB3Y4-, CDB3Y5-, CDB3Y6-Fetal interactions with corresponding incompressibilities K0 = 188, 204, 217,228, 241 and 252 MeV respectively, in excellent agreement with those of the M3Y-Paris and M3Y-Reid effective interactions. For asymmetric nuclear matter, the new set of interactions has produced the symmetry energy Esym = 32.00 MeV with an associated slope parameter L = 55 MeV at a saturation density ρ = 0.17fm−3 and asymmetry parameter δ = 1.00 (pure neutron matter) in good agreement with the standard values obtained from coupled channel analysis of charge exchange reactions, statistical multifragmentation model and terrestrial Nuclear Physics experimental analyses. Furthermore, the new set of interactions has been found to have bright prospects in a nuclear reaction as the real folded potential computed with the CDB3Y6-Fetal interaction within the framework of double folding potential has been found to be good and similar to that of CDB3Y6-Paris whose optical potential has a repulsive direct component.
La videolaringoscopia es una alternativa segura en el paciente despierto con vía aérea difícil anticipada. Reporte de Caso
Authors: Jiménez-Orduz Andrea , Camelo-Pardo Gianmarco , Archila-Tibaduiza Leidy Johanna , Orozco-Castillo María Camila , Benito-Cuadrado Luis Fernando
Published: June 2023

Introducción. El manejo de la vía aérea difícil anticipada es un reto anestésico que supone la valoración preoperatoria de las características anatómicas y los factores de riesgo específicos del paciente. La intubación difícil se presenta en 1.6 de 1,000 eventos y la intubación fallida en 0.06 de 1,000 eventos. El objetivo de este reporte es mostrar la importancia del uso de dispositivos (específicamente videolaringoscopio) en pacientes con predicción de vía aérea difícil. Presentación del caso. Hombre de 63 años con gran masa facial con extensión a nariz, labio superior, erosión a nivel del maxilar superior que ocupaba cavidad oral, con predictores de ventilación e intubación difícil, programado para rinectomía, osteotomía Lefort II, reconstrucción y traqueostomía, con intubación exitosa con videolaringoscopio en primer intento bajo sedación consciente y ventilación espontánea. Discusión. La vía aérea difícil es un escenario relacionado a factores externos e internos del paciente y a complicaciones pre e intraoperatorias. El videolaringoscopio es una herramienta útil que permite la intubación exitosa y disminuye los posibles eventos adversos (como se observó en el paciente del caso presentado) y es descrito en diferentes reportes de casos con compromiso parcial o total de la vía aérea. Conclusión. La videolaringoscopia, en casos de vía aérea difícil anticipada, está asociada con un menor tiempo de intubación, un buen perfil de seguridad y una alta tasa de éxito, comparable a la del fibrobroncoscopio, se logra la intubación en el primer intento en la mayoría de las veces y, por ello, debería considerarse como primera opción.
Antropodicea posthumana
Authors: Lamus-Gamboa Laura Vanessa
Published: June 2023

La inteligencia artificial ha avanzado rápidamente en los últimos años, recientemente han incrementado en número aquellas que se dedican a generar imágenes, recrear pinturas y crear diseños que reducen el tiempo a la hora de hacer una pieza gráfica. Hace unos meses atrás, en redes sociales se adelantó una campaña en contra del uso de las inteligencias artificiales en el campo de la ilustración digital, dicha campaña se cobijó bajo el hashtag #supporthumanartist y fue un grito de la comunidad de artistas por hacer respetar su trabajo, valorar el derecho de autoría y considerar los años de esfuerzo que supone formar un estilo, yo misma participé de esta postura en contra de las IA.
Comparison between three different protocols for isolation and culture of mouse bone marrow derived mesenchymal stem cells
Authors: AL-Qaisy Baydaa A.
Published: June 2023

Bone marrow derived mesenchymal stem cells (BM-MSCs) represent a promising source for cell therapy, As they can differentiate into bone, cartilage, fat, tendon and many other organ progenitor cells. Although the culture of MSCs has been studied for over 30 years. The identification of standard protocol for isolation and characterization have yet to be developed. A comparison study was made on three different isolation techniques, which include: direct plating, red blood lysis buffer strategies, and density gradient (DG) method (using two different gradient media: Ficoll and Percoll) to find the optimal isolation and culture condition for adequate amount of MSCs for clinical use.The results demonstrate that direct plating of whole bone marrow (BM) suspension provides a suitable alternative protocol for isolation of BM-MSCs with minimal requirements, which mainly based on the frequent medium change in primary culture. The BM crud cell suspension were isolated by aspiration from albino mice and cultured in fresh complete isolation media (CIM) RPMI-1640/10%FBS. Adherent cells from BM cells suspension were MSCs which then expanded by complete expansion media (CEM) α-MEM/ 10% FBS. By concluding, direct plating of whole bone marrow represent the alternative method for isolating of mesenchymal stem cells from small specimen of bone marrow. By concluding, the present study was designed to investigate the optimal technique for the isolation of BM-MSCs for use in tissue engineering and regenerative medicine.
Isolationand Identification of mouse bone marrow derived mesenchymal stem cells
Authors: AL-Qaisy Baydaa A.
Published: June 2023

Bone marrow derived mesenchymal stem cells (BM-MSCs) were successfully used in regenerative medicine. The purpose of this study was to isolate and characterize mesenchymal stem cells from mouse bone marrow for their subsequent use in researches. Previous data suggest that BM-MSCs are typically enriched by plastic adherent cultures, fibroplastoid cell fraction. However, Identification of MSCs achieved through their morphology, phenotypic characteristics and their biological behavior. The cellular morphology played a major part in identifying MSCs in vitro. In general, immature MSCs appeared as small, spindle-shaped cells, whereas mature MSCs was displayed as larger cells with a flat, polygonal morphology. Cells also tended to be locally confluent, growing in distinct colonies. Immunophenotypic analysis demonstrated that mouse BM-MSCs at passage three uniformly positive for CD44, CD90, CD105 and CD106. However, MSCs were always found to be negative for heamatopoietic specific markers CD34, CD45, and endothelial marker CD31. By concluding, mesenchymal stem cells can be successfully derived from mouse bone marrow by direct plating method. These cells represent a valuable source of stem cells for restoring the damaged organs
Seroprevalence of EBV in children with acute lymphoblastic leukemia
Authors: Khaleel Khaled J.
Published: June 2023

Acute lymphoblastic leukemia (ALL) accounts for about 25 percent of all childhood cancers and 75–85 % of all childhood leukemias. Thirty six children with acute lymphoblastic leukaemia were tested for antibodies to Epstein-Barr (EBV) virus with age ranged (2-12) years (20 males and 16 females) attending pediatric central hospital, oncology-hematology department .Thirty children with age ranged (2-12) years were regarded as control group. The two groups undergo Epstein-Barr virus antibodies assays (EBV-EAIgG, IgM, EBV-VCAIgG, and IgM) by enzyme linked immunosorbent assay (ELISA). The samples analysis was observed that the percentage of VCA-IgG (44.4%), VCA IgM (2.7%), EA IgG (27.7%) and EA IgM (2.7%) were higher than control group. The mean values of EBV (VCA IgG) in ALL group increase significantly when compared with control group, while no significant difference between ALL and control in the mean values of VCA IgM. There was a significant increase in the mean of EA IgG in ALL than in control group, whereas, no significant differences were noticed in EA IgM between two groups. These results clearly indicated that provide a working hypothesis and some facts on which further investigations of etiology of ALL be planned.
Diluted concentrations of large (above one hundred nanometer) silver nanoparticles inhibited the growth of different types and origin of cancer cells
Authors: Tawfeeq Amer T.
Published: June 2023

This study describes utilization of large silver nanoparticles (above one hundred nanometer in diameter) synthesized by plus laser ablation method in liquid (PLAL) in growth inhibition and apoptosis induction of cancer cells. Two different types of cancer cell lines and two different types of transformed cell lines were used. These cell lines were Rabdomyosarcoma (RD), glioblastoma (AMGM), rat embryo fibroblast transformed cell line (REF) and green monkey kidney transformed cell line (VERO). Cells were challenged with diluted concentration of silver nanoparticles they were 62.5µg/ml, 30.25µg/ml, and 15.12µg/ml. The synthesized silver nanoparticles was characterized it had surface plasmon resonance peak at 411nm and particle size distribution average diameter of 193.19 nm. Growth inhibition assay revealed that these relatively large nanoparticles are lethal toward cells under investigation; this lethality was concentration dependant during 24 hours of incubation at 37°C. According to acridine orange/propidium iodide mixed fluorescent staining assay, the used concentrations were able to induce apoptosis in all cells tested. However, the highest concentration was able to induce highest apoptosis percentage in the treated cells. This was confirmed by DNA fragmentation assay as well. In conclusion this study determined that above-one hundred nanometer size silver nanoparticle is capable of inducing apoptosis in cancer cell lines.
Human Sexual Dimorphism in 2D:4D Digit Ratio & Brain Sex: Hormones vs. Genes.
Authors: Saleh Saad , M.
Published: June 2023

I n human hands, the relative lengths of the second (2D, or index) finger and fourth (4D, or ring) finger differ between males and females. The ratio of 2D length to 4D length known as the 2D:4D ratio, & its value is < 1 for most men and ≥ 1 for most women. This sexually dimorphic character of the limb was linked to sex steroids &genes. Fetal brain on the other hand develops in the male direction (during the intrauterine period) through a direct action of testosterone on the developing nerve cells, or in the female direction through the absence of this hormone surge. The objective of this study is to search whether there is any relationship between brain sex as determined by standard scientific brain sex tests & the 2D:4D ratio by measuring the length of index & ring fingers in human hands. A sample of 150 individual (80 male &70 female) aged 20-25 years old was tested in this study. The results showed a high significant difference in 2D:4D ratio between male & female(X ²= 42.75, P< 0.001, 1df.), & the masculine brain sex score was more obvious in males with low 2D:4D digit ratio in comparison with females (X2 = 6.653, P< 0.05, 1df.).The mean brain score in males with longer 2D than4D (10.33 ±2.000) is set near the lower border of the feminine brain score (10.70 ±2.115) with a significant statistical difference (X2=8.972,P<0.05, 1df.).
Genotoxicity of Silver Nanoparticles synthesized by Laser Ablation Method in Vivo
Authors: Tawfeeq Amer T.
Published: June 2023

This research was conducted to evaluate three main methods usually used to assess cellular DNA damage in genotoxicity assays. These methods were single cell gel electrophoresis (comet assay), micronucleus formation, and DNA fragmentation assay. Nanoparticles genotoxicity is a subject of compelling immediate action as a result of wide application of nanotechnology in many sectors which in contact with humane health. The mentioned methods were used to assess the genotoxicity of silver nanoparticles in vivo. Silver nanoparticles were synthesized by laser ablation of pure silver plate submerged in double distilled water. The synthesized nanosilver was characterized with UV-Visible spectroscopy and atomic force microscope. After its characterization, silver nanoparticles were injected subcutaneously in to BALB/c mice at 200 µg/ kg BW for two different periods of time, one week and two weeks in daily manner. After the end of injection the animals were sacrificed and their bone marrow cells, lymphocytes, and spleen cells were collected. DNA damage in these cells was assessed using the three mentioned methods. Results indicated that the three types of DNA damage assessment methods were capable to detect the genotoxicity of silver nanoparticles in the treated animals. Spleen cells were the less DNA damaged cells as indicated with the three assays in the first week of injection. Lymphocytes and bone marrow cells was effected in more aggressive manner.
Association of CCND1 and HR-HPV16/18 in endometrial tumors
Authors: Mohammed Ali Saad Hasan
Published: June 2023

Background: A number of reports have demonstrated the presence of HPV in endometrial adenocarcinomas(ECs) . alterations in Cyclin D1(CCND1) gene expression has been reported in ECs and the integration of human papilloma virus (HPV)could alter the level of gene expression of CCND1. Methodology: This study has used in situ hybridization to localized HR-HPV16/18 in tissue specimens from 70 hysterectomized patients diagnosed with malignant endometrial tumors (30 cases), non-malignant endometrial tumors (25 cases),and 15 cases as control tissues groups. Also the study has enrolled detection the gene expression of CCND1 using Immunohistochemistry. Results:In the cancer sites malignant endometrial tumors group, HR-HPV16/18 was detected in 10 cases(33.3%) ,and in 5 cases (20%) non-malignant endometrial tumors ,and 4 cases (26.7%)of control tissues group. Most HPV16/18 infections have Punctate DNA pattern .The results of moleculare detection of CCND1 revealed 13(43.3%) in malignant endometrial tumor 8(32%) non-malignant endometrial tumor and 6(40%) in control groups. The significant correlation between CCND1 expression and HR-HPV16/18 infection was reported only in malignant endometrial tumors groups. Conclusions: HR-HPV16/18 may be associated with initiation of endometrial carcinogenesis events as well as play a role in the progression of such malignant tumors.CCND1 could have sharing in early events of tumorgenesis in endometrial carcinoma and a significant correlations of CCND1 expressions with HPV infection was observed among malignant endometrial tumor.