bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Water Temperature Changes on Water Loss Monitoring in Large District Heating Systems
Authors: Niemyjski Olgierd , Zwierzchowski Ryszard
Published: April 2021

This paper explores how water temperature changes in a district heating system (DHS) impact the monitoring of water losses. Water volume in DHS is constantly monitored, recorded, and replenished. The leakage and failure status of the DHS is often monitored through measuring the make-up water flow rate. In this paper, we present the methodology and a simplified model of the dynamics of the heating system operation, which was used to determine the profile of changes in the average temperature and density of water in the system. The mathematical model of the district heating network (DHN) was verified by comparing the results of simulation calculations, i.e., calculated values of the temperature of water returning to the heat source, with the measured values. Fluctuations in water temperature cause changes in the density and volume of water in the DHN, which affect the amount of water supplementing the system. This is particularly noticeable in a DHN with a large water volume. The study reports an analysis of measurement results of operating parameters of a major DHS in Poland (city of Szczecin). Hourly measurements were made of supply and return water temperature, water flow rate, and pressure throughout the whole of 2019. The water volume of the analyzed DHN is almost 42,000 m3 and the changes in water volume per hour are as high as 5 m3/h, representing 20–30% of the value of the make-up water flow rate. The analysis showed that systems for monitoring the tightness of the DHS and detecting failures, on the basis of measurements of the make-up water flow rate, should take into account the dynamics of water volume changes in the DHN.
The Future Role of Forest-Based Biofuels: Industrial Impacts in the Nordic Countries
Authors: Ogner Jåstad Eirik , Folsland Bolkesjø Torjus , Rørstad Per Kristian , Midttun Atle , Sandquist Judit , Trømborg Erik
Published: April 2021

This study applies a partial equilibrium forest sector model to analyse the impacts of biofuel deployment for road transport in the Nordic countries, when alternative use of the biomass resources and transport sector electrification are considered. We foresee a strong electrification of the transport sector, resulting in a demand for biofuels of approximately 2.5 billion L in 2035 and 1 billion L in 2050 in a 100% fossil-free base scenario. The simultaneous increase in demand from pulping industries and biofuel will cause an overall increase in wood use, of which the biofuels share will constitute approximately 20–25%. The utilization of harvest residues will increase more than 300% compared to the current level, since biofuel production will reallocate some of the current raw material used in district heating. Biofuel consumption in road transport will likely reduce after 2040 due to increasing electrification, but it is plausible that the declining domestic demand will be replaced by increasing demand from international biofuel markets in aviation and shipping. The main uncertainties in the scenarios are the future costs and profitability of forest-based biofuel technologies and the public acceptance of the close to 100 TWh of new renewable electricity production needed for the electrification of Nordic road transport.
The Effects of Hydropower Plants on the Physicochemical Parameters of the Bystrzyca River in Poland
Authors: Tomczyk Paweł , Wiatkowski Mirosław
Published: April 2021

Currently, the literature lacks comprehensive studies on the impact of hydropower plants (HPs) on the environment, including studies focused on the physicochemical parameters of water. The aim of the article is to verify the current state of knowledge on the impact of run-of-river HPs on 17 physicochemical parameters of water. The article is in line with the recommendations of the European Union that the member states, under the common energy policy, should increase the share of renewable energy sources in the energy and perform environmental impact assessments of such facilities. As a result of the analysis carried out on three HPs (Sadowice, Skałka and Marszowice) located on the Bystrzyca River (a tributary of the Odra River in Poland), it was found that HPs affect the selected physicochemical parameters of the water, i.e., (p < 0.05): electrolytic conductivity (EC; Skałka, Marszowice HPs), pH (Skałka, Marszowice HPs); nitrate nitrogen (NO3-N; Marszowice HP), dissolved oxygen (DO; Marszowice HP) and ammonium nitrogen (NH4-N; Marszowice HP). The largest (>5%), statistically significant mean cumulative effect below Marszowice HP concerned NH4-N (−27.83%), DO (+14.04%) and NO3-N (+5.50%). In addition, it was observed that the effect of HPs increases in direct proportion to the damming height, and that run-of-river HPs have a lesser impact on the physicochemical parameters’ values than in storage HPs. Our results were in accordance with those of other scientists in terms of the increase in DO, the decrease in EC, and the decrease in total phosphorus concentrations below HPs.
Coaxial Circular Jets—A Review
Authors: van Hout René , Murugan Sudharson , Mitra Abhijit , Cukurel Beni
Published: April 2021

This review article focuses on the near-field flow characteristics of coaxial circular jets that, despite their common usage in combustion processes, are still not well understood. In particular, changes in outer to inner jet velocity ratios, ru, absolute jet exit velocities and the nozzle dimensions and geometry have a profound effect on the near-field flow that is characterized by shear as well as wake instabilities. This review starts by presenting the set of equations governing the flow field and, in particular, the importance of the Reynolds stress distributions on the static pressure distribution is emphasized. Next, the literature that has led to the current stage of knowledge on coaxial jet flows is presented. Based on this literature review, several regions in the near-field (based on ru) are identified in which the inner mixing layer is either governed by shear or wake instabilities. The latter become dominant when ru≈1. For coaxial jets issued into a quiescent surrounding, shear instabilities of the annular (outer) jet are always present and ultimately govern the flow field in the far-field. We briefly discuss the effect of nozzle geometry by comparing the flow field in studies that used a blockage disk to those that employed thick inner nozzle lip thickness. Similarities and differences are discussed. While impinging coaxial jets have not been investigated much, we argue in this review that the rich flow dynamics in the near-field of the coaxial jet might be put to an advantage in fine-tuning coaxial jets impinging onto surfaces for specific heat and mass transfer applications. Several open questions are discussed at the end of this review.
A Machine Learning Method for the Fine-Grained Classification of Green Tea with Geographical Indication Using a MOS-Based Electronic Nose
Authors: Yu Dongbing , Gu Yu
Published: April 2021

Chinese green tea is known for its health-functional properties. There are many green tea categories, which have sub-categories with geographical indications (GTSGI). Several high-quality GTSGI planted in specific areas are labeled as famous GTSGI (FGTSGI) and are expensive. However, the subtle differences between the categories complicate the fine-grained classification of the GTSGI. This study proposes a novel framework consisting of a convolutional neural network backbone (CNN backbone) and a support vector machine classifier (SVM classifier), namely, CNN-SVM for the classification of Maofeng green tea categories (six sub-categories) and Maojian green tea categories (six sub-categories) using electronic nose data. A multi-channel input matrix was constructed for the CNN backbone to extract deep features from different sensor signals. An SVM classifier was employed to improve the classification performance due to its high discrimination ability for small sample sizes. The effectiveness of this framework was verified by comparing it with four other machine learning models (SVM, CNN-Shi, CNN-SVM-Shi, and CNN). The proposed framework had the best performance for classifying the GTSGI and identifying the FGTSGI. The high accuracy and strong robustness of the CNN-SVM show its potential for the fine-grained classification of multiple highly similar teas.
Influence of pH, Temperature and Protease Inhibitors on Kinetics and Mechanism of Thermally Induced Aggregation of Potato Proteins
Authors: Andlinger David J. , Röscheisen Pauline , Hengst Claudia , Kulozik Ulrich
Published: April 2021

Understanding aggregation in food protein systems is essential to control processes ranging from the stabilization of colloidal dispersions to the formation of macroscopic gels. Patatin rich potato protein isolates (PPI) have promising techno-functionality as alternatives to established proteins from egg white or milk. In this work, the influence of pH and temperature on the kinetics of PPI denaturation and aggregation was investigated as an option for targeted functionalization. At a slightly acidic pH, rates of denaturation and aggregation of the globular patatin in PPI were fast. These aggregates were shown to possess a low amount of disulfide bonds and a high amount of exposed hydrophobic amino acids (S0). Gradually increasing the pH slowed down the rate of denaturation and aggregation and alkaline pH levels led to an increased formation of disulfide bonds within these aggregates, whereas S0 was reduced. Aggregation below denaturation temperature (Td) favored aggregation driven by disulfide bridge formation. Aggregation above Td led to fast unfolding, and initial aggregation was less determined by disulfide bridge formation. Inter-molecular disulfide formation occurred during extended heating times. Blocking different protein interactions revealed that the formation of disulfide bond linked aggregation is preceded by the formation of non-covalent bonds. Overall, the results help to control the kinetics, morphology, and interactions of potato protein aggregation for potential applications in food systems.
Antioxidant Properties of Olive Mill Wastewater Polyphenolic Extracts on Human Endothelial and Vascular Smooth Muscle Cells
Authors: Posadino Anna Maria , Cossu Annalisa , Giordo Roberta , Piscopo Amalia , Abdel-Rahman Wael M. , Piga Antonio , Pintus Gianfranco
Published: April 2021

This work aims to analyze the chemical and biological evaluation of two extracts obtained by olive mill wastewater (OMW), an olive oil processing byproduct. The exploitation of OMW is becoming an important aspect of development of the sustainable olive oil industry. Here we chemically and biologically evaluated one liquid (L) and one solid (S) extract obtained by liquid–liquid extraction followed by acidic hydrolysis (LLAC). Chemical characterization of the two extracts indicated that S has higher phenol content than L. Hydroxytyrosol and tyrosol were the more abundant phenols in both OMW extracts, with hydroxytyrosol significantly higher in S as compared to L. Both extracts failed to induce cell death when challenged with endothelial cells and vascular smooth muscle cells in cell viability experiments. On the contrary, the higher extract dosages employed significantly affected cell metabolic activity, as indicated by the MTT tests. Their ability to counteract H2O2-induced oxidative stress and cell death was assessed to investigate potential antioxidant activities of the extracts. Fluorescence measurements obtained with the reactive oxygen species (ROS) probe H2DCF-DA indicated strong antioxidant activity of the two OMW extracts in both cell models, as indicated by the inhibition of H2O2-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate LLAC-obtained OMW extracts as a safe and useful source of valuable compounds harboring antioxidant activity.
Multi-Way Analysis Coupled with Near-Infrared Spectroscopy in Food Industry: Models and Applications
Authors: Yu Huiwen , Guo Lili , Kharbach Mourad , Han Wenjie
Published: April 2021

Near-infrared spectroscopy (NIRS) is a fast and powerful analytical tool in the food industry. As an advanced chemometrics tool, multi-way analysis shows great potential for solving a wide range of food problems and analyzing complex spectroscopic data. This paper describes the representative multi-way models which were used for analyzing NIRS data, as well as the advances, advantages and limitations of different multi-way models. The applications of multi-way analysis in NIRS for the food industry in terms of food process control, quality evaluation and fraud, identification and classification, prediction and quantification, and image analysis are also reviewed. It is evident from this report that multi-way analysis is presently an attractive tool for modeling complex NIRS data in the food industry while its full potential is far from reached. The combination of multi-way analysis with NIRS will be a promising practice for turning food data information into operational knowledge, conducting reliable food analyses and improving our understanding about food systems and food processes. To the best of our knowledge, this is the first paper that systematically reports the advances on models and applications of multi-way analysis in NIRS for the food industry.
Impact of COVID-19 Pandemic on Willingness to Consume Insect-Based Food Products in Catalonia
Authors: Khalil Reine , Kallas Zein , Haddarah Amira , El Omar Fawaz , Pujolà Montserrat
Published: April 2021

Edible insects are being considered as a sustainable source of protein and are continuously appearing in markets in the West. The impact of COVID-19 on the willingness to consume (WTC) two products enriched with insect ingredients, jam and yogurt, was analyzed. A semistructured questionnaire was applied using the Qualtrics© consumer panel. Data was collected from 799 and 481 consumers before and during the COVID-19 lockdown in Catalonia (Spain), respectively. The multinomial logit (MNL) model was used to analyze the determinant factors affecting consumers’ WTC insect-based products and the impact of COVID-19 on such heterogeneity. Results showed that the outbreak of COVID-19 caused a significant decrease in the WTC. Findings also revealed that consumers who contracted the COVID-19, strictly followed the regulations during the confinement, and are well informed about symptoms were more likely to reject the consumption of the insect-based products. Both before and during the lockdown, results showed that young and employed consumers, with low-income level, who give importance to the environmental attribute in food are prone to consume insect-based food products. The COVID-19 outbreak had a homogenizing impact on consumers’ WTC with respect to the gender variable. Consumers’ affirmation towards strict food safety standards of the insect-based products should be remarked.
CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells
Authors: Malik Radek , Svoboda Petr
Published: April 2021

RNA interference (RNAi) designates sequence-specific mRNA degradation mediated by small RNAs generated from long double-stranded RNA (dsRNA) by RNase III Dicer. RNAi appears inactive in mammalian cells except for mouse oocytes, where high RNAi activity exists because of an N-terminally truncated Dicer isoform, denoted DicerO. DicerO processes dsRNA into small RNAs more efficiently than the full-length Dicer expressed in somatic cells. DicerO is expressed from an oocyte-specific promoter of retrotransposon origin, which is silenced in other cell types. In this work, we evaluated CRISPR-based strategies for epigenetic targeting of the endogenous Dicer gene to restore DicerO expression and, consequently, RNAi. We show that reactivation of DicerO expression can be achieved in mouse embryonic stem cells, but it is not sufficient to establish a robust canonical RNAi response.
Mapping Evidence of Impacts of COVID-19 Outbreak on Sexual and Reproductive Health: A Scoping Review
Authors: Bolarinwa Obasanjo Afolabi , Ahinkorah Bright Opoku , Seidu Abdul-Aziz , Ameyaw Edward Kwabena , Saeed Balsam Qubais , Hagan John Elvis , Nwagbara Ugochinyere Ijeoma
Published: April 2021

Introduction: The emergence of the coronavirus disease 2019 (COVID-19) pandemic has rapidly transformed the pre-existing worldwide sexual and reproductive health environment. The provision and supply of contraceptives, and a wide variety of sexual health, new-born, and maternal health services have been seriously affected. Thus, this scoping review mapped the available evidence on the impacts of the COVID-19 outbreak on sexual and reproductive health. Methods: Arksey and O’Malley’s methodological framework guided this scoping review. A search was conducted from the following databases: Embase, PubMed, CINAHL, Scopus, WOS, and AJOL. The preferred reporting items for systematic reviews and meta-analyses (PRISMA) chart and PRISMA extension for scoping reviews (PRISMA-ScR) checklist were used to document the review process. The McMaster critical review checklist was used to determine the quality of the included studies. Thematic analyses were conducted using NVivo version 12. Results: Three studies showed evidence on the impact of COVID-19 and family planning services, six studies reported on maternal and child services and eleven studies reported on sexual health (sexual behavior). Limited access to family planning use, reduction in multiple sexual partnership, decreased transactional sex, and maternal and child services disruption were some impacts reported in the included studies. Conclusion: This study has demonstrated the impacts of COVID-19 on family planning access, multiple sexual partnership, transactional sex, and disruption of maternal and child health services. Interventions that will consider the immediate availability of and access to all sexual and reproductive health services should be prioritized.
Neuroenhancement in French and Romanian University Students, Motivations and Associated Factors
Authors: Brumboiu Irina , Porrovecchio Alessandro , Peze Thierry , Hurdiel Remy , Cazacu Irina , Mogosan Cristina , Ladner Joel , Tavolacci Marie-Pierre
Published: April 2021

This cross-sectional study aimed to determine the use of neuroenhancers, the motivations and factors associated with their use in French and Romanian university students. Students from two universities in France (Rouen and Opal Coast University) and one in Romania (Cluj-Napoca) were asked to complete a self-administered anonymous questionnaire, either online or on paper, about the use of three different categories of substance: Prescription drugs (methylphenidate, modafinil, and beta-blockers), drugs of abuse (alcohol, cannabis, cocaine, and amphetamines), and soft enhancers (coffee, vitamins, caffeine tablets, and energy drinks). In total, 1110 students were included: The users were 2.2% for prescription drugs, 4.3% for drugs of abuse, and 55.0% for soft enhancers. Students used neuroenhancement to stay awake for study (69.3%), to improve concentration (55.5%), to decrease stress (40.9%), and to improve memory (39.6%). Neuroenhancement was considered to meet expectations by 74.4% of users. The factors associated with the use of drugs of abuse were frequent binge drinking (Adjusted Odds Ratio—AOR: 6.49 [95% CI: 2.53–16.6]), smoking (AOR: 5.50 [95% CI: 2.98–10.14]), having a student job (AOR: 2.42 [95% CI 1.13–5.17]), and being male (AOR: 2.23 [95% CI:1.21–4.11]). No significant associations with eating disorders were detected for any of the three categories of substances. University students reported neuroenhancement with prescription drugs, drugs of abuse, and mainly soft enhancers. These substances were used mainly to increase the waking hours. Educational programs in universities seem to be required in order to increase student awareness of the problems caused by neuroenhancements, and to decrease the associated risks by changing students’ attitudes and beliefs.
Fly Ash Waste Recycling by Pt/TiO2 Incorporation for Industrial Dye Removal
Authors: Visa Maria , Cosnita Mihaela , Moldovan Macedon , Marin Cosmina Andreea , Mihaly Maria
Published: April 2021

New materials are obtained by transforming fly ash wastes into a valuable composite, with tandem adsorption and photodegradation properties. Mild hydrothermal synthesis, from titanium dioxide, platinum nanoparticles and zeolite materials obtained from a waste, fly ash, as support, was involved in the composite preparation. The platinum nanoparticles extended the photocatalytic activity of the composite in visible range (Eg = 2.1 eV). The efficiency of tandem adsorption and photocatalytic activity of the new composite were determined to be 25% for Bemacid Blau and 43.89% for Bemacid Rot after 360 min, the irradiation time. The addition of H2O2 improves the process efficiency up to 80.70% and 93.43%, respectively. The Pt nanoparticle (PtNP) contribution led to the band gap energy change to Vis light (400 nm), thus suggesting the possibility of photocatalysis under the action of a fraction of natural light.
Exploring Quality of Life, Stress, and Risk Factors Associated with Irritable Bowel Syndrome for Female University Students in Taiwan
Authors: Chen Huan-Hwa , Hung Chich-Hsiu , Kao Ai-Wen , Hsieh Hsiu-Fen
Published: April 2021

Irritable bowel syndrome (IBS) is a common recurrent functional gastrointestinal disorder that impacts on patients physically and mentally. Studies on IBS have focused on adults, yet few studies have examined IBS among female university students. The aim of this study was to investigate the prevalence of IBS for female university students and its related factors. Using a cross-sectional study design, a total of 2520 female university students were recruited in southern Taiwan. The structured questionnaires, including the Rome III IBS diagnostic questionnaire, IBS symptom severity scale, Perceived Stress Scale, and World Health Organization Quality of Life BREF questionnaire (WHOQOL-BREF) were used for data collection. A total of 1894 female students complete the questionnaires. The response rate was 75.15%. The results indicated 193 female students with IBS and the prevalence of IBS was 10.1%. IBS female students had higher levels of stress and lower QOL than non-IBS female students. The risk factors for female university students developing IBS were dysmenorrhea, food avoidance, class absenteeism, and the lower physical domain of QOL. It is advised to consider these factors when providing students with counselling and relevant services in the expectation of alleviating their IBS symptoms, reducing the incidence rate of IBS, and further improving their QOL.
The Effects of a Passive Exoskeleton on Human Thermal Responses in Temperate and Cold Environments
Authors: Liu Yang , Li Xiaoling , Lai Jiarui , Zhu Aibin , Zhang Xiaodong , Zheng Ziming , Zhu Huijin , Shi Yueyang , Wang Long , Chen Zhangyi
Published: April 2021

The exoskeleton as functional wearable equipment has been increasingly used in working environments. However, the effects of wearing an exoskeleton on human thermal responses are still unknown. In this study, 10 male package handlers were exposed to 10 °C (COLD) and 25 °C (TEMP) ambient temperatures while performing a 10 kg lifting task (LIFTING) and sedentary (REST) both with (EXO) and without the exoskeleton (WEXO). Thermal responses, including the metabolic rate and mean skin temperature (MST), were continuously measured. Thermal comfort, thermal sensation and sweat feeling were also recorded. For LIFTING, metabolic heat production is significant decrease with the exoskeleton support. The MST and thermal sensation significantly increase when wearing the exoskeleton, but thermal discomfort and sweating are only aggravated in TEMP. For REST, MST and thermal sensation are also increased by the exoskeleton, and there is no significant difference in the metabolic rate between EXO and WEXO. The thermal comfort is significantly improved by wearing the exoskeleton only in COLD. The results suggest that the passive exoskeleton increases the local clothing insulation, and the way of wearing reduces the “pumping effect”, which makes a difference in the thermal response between COLD and TEMP. Designers need to develop appropriate usage strategies according to the operative temperature.
Impact of COVID-19 on Healthcare Labor Market in the United States: Lower Paid Workers Experienced Higher Vulnerability and Slower Recovery
Authors: Bhandari Neeraj , Batra Kavita , Upadhyay Soumya , Cochran Christopher
Published: April 2021

The resilience of the healthcare industry, often considered recession-proof, is being tested by the COVID-19 induced reductions in physical mobility and restrictions on elective and non-emergent medical procedures. We assess early COVID-19 effects on the dynamics of decline and recovery in healthcare labor markets in the United States. Descriptive analyses with monthly cross-sectional data on unemployment rates, employment, labor market entry/exit, and weekly work hours among healthcare workers in each healthcare industry and occupation, using the Current Population Survey from July 2019−2020 were performed. We found that unemployment rates increased dramatically for all healthcare industries, with the strongest early impacts on dentists’ offices (41.3%), outpatient centers (10.5%), physician offices (9.5%), and home health (7.8%). Lower paid workers such as technologists/technicians (10.5%) and healthcare aides (12.6%) were hit hardest and faced persistently high unemployment, while nurses (4%), physicians/surgeons (1.4%), and pharmacists (0.7%) were spared major disruptions. Unique economic vulnerabilities faced by low-income healthcare workers may need to be addressed to avoid serious disruptions from future events similar to COVID-19.
A Systematic Review to Examine the Evidence in Developing Social Prescribing Interventions That Apply a Co-Productive, Co-Designed Approach to Improve Well-Being Outcomes in a Community Setting
Authors: Thomas Gwenlli , Lynch Mary , Spencer Llinos Haf
Published: April 2021

This systematic review aims to investigate the evidence in applying a co-design, co-productive approach to develop social prescribing interventions. A growing body of evidence suggests that co-production and co-design are methods that can be applied to engage service users as knowledgeable assets who can contribute to developing sustainable health services. Applying the Preferred Reporting Items for Systemic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic literature search was conducted. Peer-reviewed articles were sought using electronic databases, experts and grey literature. The review search concluded with eight observational studies. Quality appraisal methods were influenced by the Grades of Recommendation, Assessment, Development and Evaluation (GRADE) Framework approach. A narrative thematic synthesis of the results was conducted. The evidence suggests that a co-design and co-productive social prescribing can lead to positive well-being outcomes among communities. Barriers and facilitators of co-production and co-design approach were also highlighted within the evidence. The evidence within this review confirms that a co-production and co-design would be an effective approach to engage stakeholders in the development and implementation of a SP intervention within a community setting. The evidence also implies that SP initiatives can be enhanced from the outset, by drawing on stakeholder knowledge to design a service that improves health and well-being outcomes for community members.
Device-Measured and Self-Reported Active Travel Associations with Cardiovascular Disease Risk Factors in an Ethnically Diverse Sample of Adults
Authors: Crist Katie , Benmarhnia Tarik , Zamora Steven , Yang Jiue-An , Sears Dorothy D. , Natarajan Loki , Dillon Lindsay , Sallis James F. , Jankowska Marta M.
Published: April 2021

Active travel (AT) provides an opportunity to alleviate the physical inactivity and climate crises contributing to the global chronic disease burden, including cardiovascular diseases (CVD). Though AT shows promising links to reduced CVD risk, prior studies relied on self-reported AT assessment. In the present study, device-measured and self-reported AT were compared across population subgroups and relationships with CVD risk biomarkers were evaluated for both measures. The study recruited an ethnically diverse sample (N = 602, mean age 59 years, 42% Hispanic/Latino ethnicity) from neighborhoods that varied by walkability and food access. AT was assessed using concurrently collected accelerometer and GPS data and self-report data from a validated survey. Relationships with body mass index (BMI), triglycerides, high-density lipoprotein (HDL) cholesterol, blood pressure (BP), and moderate-to-vigorous physical activity (MVPA) were modeled using multivariable linear regression. Devices captured more AT than did self-report. We found differences in AT measures by population subgroups, including race, ethnicity, education, income, vehicle access, and walkability. Men had more accelerometer-measured MVPA, though women self-reported more daily minutes. Both device and survey AT measures were positively associated with total accelerometer-measured MVPA, though the relationship was stronger with device-measured AT. Device-measured AT was associated with lower BMI. No other CVD risk biomarker was associated with either AT measure. No effect modification by Hispanic/Latino ethnicity was detected. Further studies with device-based measures are warranted to better understand the relationship between AT and cardiovascular health.
Detection of Free-Living Amoebae and Their Intracellular Bacteria in Borehole Water before and after a Ceramic Pot Filter Point-of-Use Intervention in Rural Communities in South Africa
Authors: van der Loo Clarissa , Bartie Catheleen , Barnard Tobias George , Potgieter Natasha
Published: April 2021

Free-living amoebae (FLA) are ubiquitous in nature, whereas amoeba-resistant bacteria (ARB) have evolved virulent mechanisms that allow them to resist FLA digestion mechanisms and survive inside the amoeba during hostile environmental conditions. This study assessed the prevalence of FLA and ARB species in borehole water before and after a ceramic point-of-use intervention in rural households. A total of 529 water samples were collected over a five-month period from 82 households. All water samples were subjected to amoebal enrichment, bacterial isolation on selective media, and molecular identification using 16S PCR/sequencing to determine ARB species and 18S rRNA PCR/sequencing to determine FLA species present in the water samples before and after the ceramic pot intervention. Several FLA species including Acanthamoeba spp. and Mycobacterium spp. were isolated. The ceramic pot filter removed many of these microorganisms from the borehole water. However, design flaws could have been responsible for some FLA and ARB detected in the filtered water. FLA and their associated ARB are ubiquitous in borehole water, and some of these species might be potentially harmful and a health risk to vulnerable individuals. There is a need to do more investigations into the health risk of these organisms after point-of-use treatment.
Effects of Early Vocal Contact in the Neonatal Intensive Care Unit: Study Protocol for a Multi-Centre, Randomised Clinical Trial
Authors: Filippa Manuela , Della Casa Elisa , D’amico Roberto , Picciolini Odoardo , Lunardi Clara , Sansavini Alessandra , Ferrari Fabrizio
Published: April 2021

Preterm infants are at risk for developing altered trajectories of cognitive, social, and linguistic competences compared to a term population. This is mainly due to medical and environmental factors, as they are exposed to an atypical auditory environment and simultaneously, long periods of early separation from their parents. The short-term effects of early vocal contact (EVC) on an infant’s early stability have been investigated. However, there is limited evidence of its impact on the infant’s autonomic nervous system maturation, as indexed by heart rate variability, and its long-term impact on infant neurodevelopment. Our multi-centric study aims to investigate the effects of EVC on a preterm infant’s physiology, neurobehaviour, and development. Eighty stable preterm infants, born at 25–32 weeks and 6 days gestational age, without specific abnormalities, will be enrolled and randomised to either an intervention or control group. The intervention group will receive EVC, where mothers will talk and sing to their infants for 10 min three times per week for 2 weeks. Mothers in the control group will be encouraged to spend the same amount of time next to the incubator and observe the infant’s behaviour through a standard cluster of indicators. Infants will be assessed at baseline; the end of the intervention; term equivalent age; and 3, 6, 12, and 24 months corrected age, with a battery of physiological, neurobehavioral, and developmental measures. Early interventions in the neonatal intensive care unit have demonstrated effects on the neurodevelopment of preterm infants, thereby lowering the negative long-term effects of an atypical auditory and interactional environment. Our proposed study will provide new insight into mother–infant early contact as a protective intervention against the sequelae of prematurity during this sensitive period of development. Early intervention, such as EVC, is intuitive and easy to implement in the daily care of preterm infants. However, its long-term effects on infant neurodevelopment and maternal sensitivity and stress are still unclear. Trial Registration: NCT04759573, retrospectively registered, 17 February 2021.
Managing a Pan-European Consortium on Late Effects among Long-Term Survivors of Childhood and Adolescent Cancer—The PanCareLIFE Project
Authors: Kaatsch Peter , Byrne Julianne , Grabow Desiree ,   on behalf of the PanCareLIFE Consortium
Published: April 2021

PanCareLIFE brought together European partners and is the largest study to have evaluated the issues of fertility impairment, hearing loss, and health-related quality of life in survivors of childhood and adolescent cancer. Successful delivery of the project aims did not evolve solely from scientific qualities. Organizational structure and careful information management were key components for its successful completion and are retrospectively assessed in this paper. PanCareLIFE used cohort studies, case-control studies, clinical evaluation of hearing, and genetic testing to study 32,000 survivors from 25 data providers. A management team implemented the organizational structures, was the decision making body, developed and maintained a communication plan, and supervised deadlines, and made timely decisions. A biostatistics support group and an ethical advisory board were established. A publication committee ensured quality and accuracy of publications and is jointly responsible for the sustainability of the project. The chosen management structure of PanCareLIFE can serve as a blueprint for the management of complex international projects. Apart from the survivors themselves, various target audiences like oncology researchers, health care providers, and policy makers can derive benefits from the project. The results can also be used in oncological frontline therapy to reduce toxicity.
COVID-19 Management in the Pediatric Age: Consensus Document of the COVID-19 Working Group in Paediatrics of the Emilia-Romagna Region (RE-CO-Ped), Italy
Authors: Esposito Susanna , Marchetti Federico , Lanari Marcello , Caramelli Fabio , De Fanti Alessandro , Vergine Gianluca , Iughetti Lorenzo , Fornaro Martina , Suppiej Agnese , Zona Stefano , Pession Andrea , Biasucci Giacomo ,   on behalf of the Working Group on COVID-19 in Pediatrics of the Emilia-Romagna Region (RE-CO-Ped)
Published: April 2021

Since December 2019, coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread, becoming the first pandemic of the 21st century by number of deaths (over 2,000,000 worldwide). Many aspects of SARS-CoV-2 infection in children and adolescents remain unclear, and optimal treatment has not yet been defined. Therefore, our goal was to develop a consensus document, practically synthesizing the accumulated data and clinical experience of our expert group. Literature research was carried out using the keywords “COVID-19” or “SARS-CoV-2” and “children” or “pediatrics” and “prevention” or “diagnosis” or “MIS-C” or “treatment” in electronic databases (MEDLINE, PUBMED), existing guidelines and gray literature. The fact that the majority of the problems posed by SARS-CoV-2 infection in pediatric age do not need hospital care and that, therefore, infected children and adolescents can be managed at home highlights the need for a strengthening of territorial pediatric structures. The sharing of hospitalization and therapeutic management criteria for severe cases between professionals is essential to ensure a fair approach based on the best available knowledge. Moreover, the activity of social and health professionals must also include the description, management and limitation of psychophysical-relational damage resulting from the SARS-CoV-2 pandemic on the health of children and adolescents, whether or not affected by COVID-19. Due to the characteristics of COVID-19 pathology in pediatric age, the importance of strengthening the network between hospital and territorial pediatrics, school, educational, social and family personnel both for strictly clinical management and for the reduction in discomfort, with priority in children of more frail families, represents a priority.
Disproportionate Impacts of Wildfires among Elderly and Low-Income Communities in California from 2000–2020
Authors: Masri Shahir , Scaduto Erica , Jin Yufang , Wu Jun
Published: April 2021

Wildfires can be detrimental to urban and rural communities, causing impacts in the form of psychological stress, direct physical injury, and smoke-related morbidity and mortality. This study examined the area burned by wildfires over the entire state of California from the years 2000 to 2020 in order to quantify and identify whether burned area and fire frequency differed across Census tracts according to socioeconomic indicators over time. Wildfire data were obtained from the California Fire and Resource Assessment Program (FRAP) and National Interagency Fire Center (NIFC), while demographic data were obtained from the American Community Survey. Results showed a doubling in the number of Census tracts that experienced major wildfires and a near doubling in the number of people residing in wildfire-impacted Census tracts, mostly due to an over 23,000 acre/year increase in the area burned by wildfires over the last two decades. Census tracts with a higher fire frequency and burned area had lower proportions of minority groups on average. However, when considering Native American populations, a greater proportion resided in highly impacted Census tracts. Such Census tracts also had higher proportions of older residents. In general, high-impact Census tracts tended to have higher proportions of low-income residents and lower proportions of high-income residents, as well as lower median household incomes and home values. These findings are important to policymakers and state agencies as it relates to environmental justice and the allocation of resources before, during, and after wildfires in the state of California.
The Enabling Role of ICT to Mitigate the Negative Effects of Emotional and Social Loneliness of the Elderly during COVID-19 Pandemic
Authors: Llorente-Barroso Carmen , Kolotouchkina Olga , Mañas-Viniegra Luis
Published: April 2021

(1) Background: The COVID-19 pandemic has been especially hard on the elderly owing to their particular vulnerability to the virus. Their confinement to prevent the spread of the virus resulted in social isolation, often linked to the unwanted loneliness that hinders their emotional well-being. The enabling capacity of ICT (Information and Communication Technology) to overcome the negative effects of this isolation requires special attention. The purpose of this research is to understand the impact of the use of ICT on the emotional well-being of elderly people during their confinement. (2) Methods: A qualitative exploration method based on four focus groups with elderly people aged 60 years or older and three in-depth personal interviews with experts in education of the elderly were carried out. (3) Results: Research results evidence a negative emotional impact of the confinement (lack of physical contact with their loved ones, fear and uncertainty, feeling of loneliness, sadness at the loss of family members) on the emotional well-being of study participants. Furthermore, the operational capacity of ICT to prevent infection, as well as their positive emotional and humanizing role in providing access to entertainment and hobbies, and in improving self-esteem was also acknowledged. (4) Conclusions: ICT have become a valuable ally for elderly people aged 60 years and older to mitigate the negative effects of social isolation and loneliness imposed by the confinement.
Knowledge-Based Attitudes of Medical Students in Antibiotic Therapy and Antibiotic Resistance. A Cross-Sectional Study
Authors: Sobierajski Tomasz , Mazińska Beata , Wanke-Rytt Monika , Hryniewicz Waleria
Published: April 2021

We aimed to evaluate the knowledge-based attitudes of antibiotics and antibiotic resistance among medical students of Medical University of Warsaw using the questionnaire prepared by the study’s authors. In May–June 2018, we carried out a cross-sectional study among the students of all years, embracing 291 respondents. The students were divided into two groups: A (students in their first to third years) and B (students in their fourth to sixth years). Our study has shown that students are aware of the dangers of antibiotic resistance, seeing the leading cause as antibiotic misuse. We have shown that they are also aware of their insufficient knowledge and believe that more antibiotic therapy classes should be included in the curriculum of Medical University of Warsaw. Our questionnaire also focused on attitudes towards antibiotics based on knowledge. One in four respondents (23.7%), based on their knowledge, negated antibiotic therapy ordered by a doctor in the event of their illness, and four in ten (40.9%) in the occurrence of disease of a family member or friend. The vast majority of students (92.4%) would like to broaden their knowledge on antibiotic therapy. However, only one-fifth of students have heard about the European Antibiotic Awareness Day campaign. We recommend increasing the number of hours on antibiotic therapy and resistance education combined with topics on hand hygiene.
Associations among Stress, Anxiety, Depression, and Emotional Intelligence among Veterinary Medicine Students
Authors: Wells Julia , Watson Kylie , E. Davis Robert , Siraj A. Quadri Syed , R. Mann Joshua , Verma Ashutosh , Sharma Manoj , Nahar Vinayak K.
Published: April 2021

Background: Veterinary students are faced with immense pressures and rigors during school. These pressures have contributed to elevated levels of stress, anxiety, and depression (SAD) among veterinary students relative to the general population. One proposed concept to help students combat SAD is that of emotional intelligence (EI). We explored the relationship between EI and SAD among veterinary students at a college in the Southeast United States. Methods: A cross-sectional study design was implemented among a convenience sample of 182 veterinary medical students. The survey instrument contained 56 items that elicited information about students’ demographics, perceived stress, anxiety, and depression, and emotional intelligence levels. Data analysis included univariate statistics, Pearson’s correlations, and multiple regression and independent samples t-tests. Results: The study revealed a statistically significant, negative correlation between EI levels and stress, anxiety, and depression. Additionally, a statistically significant, positive correlation was found between stress and anxiety as well as both stress and anxiety and depression. Multiple linear regression showed that EI was a statistically significant predictor of stress (b = −0.239, p < 0.001), anxiety (b = −0.044, p < 0.001), and depression (b = −0.063, p < 0.001), after controlling for sociodemographic variables. Students’ t-test results revealed a statistically significant mean difference in EI scores among students screening positive versus negative for depression, with students screening negative having a mean EI score of 10.81 points higher than students who screened positive for depression. Conclusion: There is a scientifically supported need for interventions in veterinary school to integrate EI into the veterinary medical curriculum and consider the EI levels of veterinary student candidates.
Correction: Weng, J., et al. DNA Methylation Analysis Identifies Patterns in Progressive Glioma Grades to Predict Patient Survival. Int. J. Mol. Sci. 2021, 22, 1020
Authors: Weng Jingyin , Salazar Nicole
Published: April 2021

The authors wish to make the following corrections to paper “DNA Methylation Analysis Identifies Patterns in Progressive Glioma Grades to Predict Patient Survival” [...]
The Influence of Cold Atmospheric Pressure Plasma-Treated Media on the Cell Viability, Motility, and Induction of Apoptosis in Human Non-Metastatic (MCF7) and Metastatic (MDA-MB-231) Breast Cancer Cell Lines
Authors: Terefinko Dominik , Dzimitrowicz Anna , Bielawska-Pohl Aleksandra , Klimczak Aleksandra , Pohl Pawel , Jamroz Piotr
Published: April 2021

Breast cancer remains the most common type of cancer, occurring in middle-aged women, and often leads to patients’ death. In this work, we applied a cold atmospheric pressure plasma (CAPP)-based reaction-discharge system, one that is unique in its class, for the production of CAPP-activated media (DMEM and Opti-MEM); it is intended for further uses in breast cancer treatment. To reach this aim, different volumes of DMEM or Opti-MEM were treated by CAPP. Prepared media were exposed to the CAPP treatment at seven different time intervals and examined in respect of their impact on cell viability and motility, and the induction of the apoptosis in human non-metastatic (MCF7) and metastatic (MDA-MB-231) breast cancer cell lines. As a control, the influence of CAPP-activated media on the viability and motility, and the type of the cell death of the non-cancerous human normal MCF10A cell line, was estimated. Additionally, qualitative and quantitative analyses of the reactive oxygen and nitrogen species (RONS), generated during the CAPP operation in contact with analyzed media, were performed. Based on the conducted research, it was found that 180 s (media activation time by CAPP) should be considered as the minimal toxic dose, which significantly decreases the cell viability and the migration of MDA-MB-231 cells, and also disturbs life processes of MCF7 cells. Finally, CAPP-activated media led to the apoptosis of analyzed cell lines, especially of the metastatic MDA-MB-231 cell line. Therefore, the application of the CAPP system may be potentially applied as a therapeutic strategy for the management of highly metastatic human breast cancer.