bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates
Authors: Travnickova Martina , Pajorova Julia , Zarubova Jana , Krocilova Nikola , Molitor Martin , Bacakova Lucie
Published: February 2020

Background. Adipose tissue-derived stromal cells (ADSCs) have great potential for cell-based therapies, including tissue engineering. However, various factors can influence the characteristics of isolated ADSCs. Methods. We studied the influence of the harvesting site, i.e., inner thigh (n=3), outer thigh (n=7), and abdomen (n=9), and of negative pressure, i.e., low (-200 mmHg) and high (-700 mmHg), on the characteristics of isolated ADSCs. We counted initial yields of attached cells after isolation. In subsequent passage, we studied the number, viability, diameter, doubling time, mitochondrial activity, and CD surface markers of isolated ADSCs. Results. We revealed higher initial cell yields from the outer thigh region than from the abdomen region. Negative pressure did not influence the cell yields from the outer thigh region, whereas the yields from the abdomen region were higher under high negative pressure than under low negative pressure. In the subsequent passage, in general, no significant relationship was identified between the different negative pressure and ADSC characteristics. No significant difference was observed in the characteristics of thigh ADSCs and abdomen ADSCs. Only on day 1, the diameter was significantly bigger in outer thigh ADSCs than in abdomen ADSCs. Moreover, we noted a tendency of thigh ADSCs (i.e., inner thigh+outer thigh) to reach a higher cell number on day 7. Discussion. The harvesting site and negative pressure can potentially influence initial cell yields from lipoaspirates. However, for subsequent in vitro culturing and for use in tissue engineering, it seems that the harvesting site and the level of negative pressure do not have a crucial or limiting effect on basic ADSC characteristics.
STEIM: A Spatiotemporal Event Interaction Model in V2X Systems Based on a Time Period and a Raster Map
Authors: Xu Cheng , Luo Hengjie , Bao Hong , Wang Pengfei
Published: February 2020

The Internet of Vehicles (IoV) is an important artificial intelligence research field for intelligent transportation applications. Complex event interactions are important methods for data flow processing in a Vehicle to Everything (V2X) environment. Unlike the classic Internet of Things (IoT) systems, data streams in V2X include both temporal information and spatial information. Thus, effectively expressing and addressing spatiotemporal data interactions in the IoV is an urgent problem. To solve this problem, we propose a spatiotemporal event interaction model (STEIM). STEIM uses a time period and a raster map for its temporal model and spatial model, respectively. In this paper, first, we provide a spatiotemporal operator and a complete STEIM grammar that effectively expresses the spatiotemporal information of the spatiotemporal event flow in the V2X environment. Second, we describe the design of the operational semantics of the STEIM from the formal semantics. In addition, we provide a spatiotemporal event-stream processing algorithm that is based on the Petri net model. The STEIM establishes a mechanism for V2X event-stream temporal and spatial processing. Finally, the effectiveness of the STEIM-based system is demonstrated experimentally.
Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking
Authors: Zhao Ting , Liu Zihui , Niu Jingmei , Lv Baoxing , Xiao Yuliang , Li Yuqin
Published: February 2020

Salbutamol (SBAL), a kind of short‐acting beta 2‐adrenergic agonist, has been mainly used to treat bronchial asthma and other allergic airway diseases clinically. In this study, the interaction mechanism between salbutamol and human serum albumin was researched by the multispectral method and molecular docking. The fluorescence intensity of HSA could be regularly enhanced with the increase of SBAL concentration. Both the results of the multispectral method and molecular docking showed that SBAL could bind HSA with van der Waals force and hydrogen bonds. The binding mechanism was further analysed by UV‐Vis and synchronous fluorescence spectra. The contents of the secondary structure of free HSA and SBAL‐HSA complex were evaluated using CD spectra.
Study on Dynamic Characteristics of the Disc Spring System in Vibration Screen
Authors: Zhou Jiacheng , Zhang Chuzhe , Wang Ziqiu , Mao Kuanmin
Published: February 2020

To avoid too large exciting force in traditional linear vibrating screen and unstable working state in resonance screen, the disc spring system is applied in the linear vibration screen. The model of the disc spring system in vibration screen is established by simulation and experiment. The characteristics of modal and amplitude of the disc spring system in vibration screen are studied. We found that the disc spring system vibrates in vertical direction at the third-order natural frequency, which is consistent with the direction of the vibration screen when screening particles. Moreover, the third-order natural frequencies in simulation and experiment are basically consistent. Furthermore, the maximum amplitude of the disc spring system appears at 960 r/min (16 Hz), which is in accord with the third-order natural frequency. Meanwhile, the amplitude increases proportionally with the increase of exciting force, while the amplification factors are the same under three different exciting forces. This indicates that the disc spring system has excellent linearity. The results of research provide guidance for design and application of elastic components on the vibration screen.
Analysis on the Effects of Material Parameters on the Fatigue Performance of Novel Anticorrugation Elastic Rail Clips
Authors: Wang Ping , Lu Jun , Zhao Caiyou , Yao Li , Ming Xuanhui
Published: February 2020

A novel elastic rail clip was obtained through size improvement based on the II-type elastic rail clips to solve the fatigue failure of elastic rail clips. The metro vehicle-track coupling model and the fastener system refinement model were developed to analyze the effects of the material (38Si7 and 60Si2Mn) on the static and dynamic responses of the elastic rail clips and their fatigue performances under rail corrugation. According to the results, the elastic rail clip made of 60Si2Mn did not undergo any plastic deformation and exhibited higher strength than that made of 38Si7. Elastic rail clips made of 38Si7 and 60Si2Mn presented a consistent clamping force, natural frequency, and vibration acceleration. However, they were most sensitive to the wavelength of 50 mm. Both materials influenced the small back bending area of the elastic rail clip only. The elastic rail clip made of 60Si2Mn could meet the fatigue life requirements of 5 million times. As for the elastic rail clip made of 38Si7, the corresponding values of corrugation with the wavelength of 50 mm and 40 mm are to be controlled below 0.09 mm and 0.41 mm, respectively. The performance of the elastic rail clips to resist rail corrugation can be improved significantly through the increase in the geometric dimensions and selection of materials with higher strength.
Edge Prior Multilayer Segmentation Network Based on Bayesian Framework
Authors: He Chu , Shi Zishan , Fang Peizhang , Xiong Dehui , He Bokun , Liao Mingsheng
Published: February 2020

In recent years, methods based on neural network have achieved excellent performance for image segmentation. However, segmentation around the edge area is still unsatisfactory when dealing with complex boundaries. This paper proposes an edge prior semantic segmentation architecture based on Bayesian framework. The entire framework is composed of three network structures, a likelihood network and an edge prior network at the front, followed by a constraint network. The likelihood network produces a rough segmentation result, which is later optimized by edge prior information, including the edge map and the edge distance. For the constraint network, the modified domain transform method is proposed, in which the diffusion direction is revised through the newly defined distance map and some added constraint conditions. Experiments about the proposed approach and several contrastive methods show that our proposed method had good performance and outperformed FCN in terms of average accuracy for 0.0209 on ESAR data set.
Electroacupuncture Alleviates Experimental Chronic Inflammatory Pain by Inhibiting Calcium Voltage‐Gated Channel‐Mediated Inflammation
Authors: Zhou Jie , Jin Ying , Ma Ruijie , Song Hongyun , Chen Qin , Chai Yueyang , Liang Yi , Zhou You , Fang Jianqiao
Published: February 2020

Background. Both experimental and clinical studies have shown that electroacupuncture (EA) administration ameliorates chronic inflammatory pain (CIP). However, the multifaceted mechanism underlying the effects of EA on CIP is poorly understood. In this study, the mRNA transcriptome was used to study various therapeutic targets of EA. Methods. Using RNA‐sequencing, protein‐coding mRNA expression profiles of the L4‐L5 dorsal root ganglion (DRG) were examined in the control (CN), complete Freund’s adjuvant‐ (CFA‐) induced CIP, and EA‐treated CIP groups. A series of bioinformatics analyses was performed; “EA‐reversed upregulated genes with CIP” (up‐DEGs) and “EA‐reversed downregulated genes with CIP” (down‐DEGs) were identified. Thereafter, based on up‐DEGs and down‐DEGs, biological functions and signaling pathways were enriched using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses. Results. In total, 189 DEGs were identified, including 134 up‐ and 55 down‐DEGs, which were enriched in arachidonic acid metabolism (rno00590), glutamatergic synapse (rno04724), serotonergic synapse (rno04726), FoxO signaling pathway (rno04068), insulin signaling pathway (rno04910), amyotrophic lateral sclerosis (rno05014), cholinergic synapse (rno04725), ECM‐receptor interaction (rno04512), and choline metabolism in cancer (rno05231). Conclusion. We identified a few GOs, pathways, and genes that could play key roles in the amelioration of CIP by EA. Hence, this study may provide a theoretical basis for CIP amelioration by EA.
A Novel Sliding Mode Control with Low-Pass Filter for Nonlinear Handling Chain System in Container Ports
Authors: Xu Bowei , Li Junjun , Yang Yongsheng , Wu Huafeng , Postolache Octavian
Published: February 2020

Nonlinearities in a container port handling chain include mainly nonnegative arrive rate of container cargoes, limited container handling completion rate, and nonnegative unsatisfied freight requirement constraints. The nonlinearity influences the operation resources availability and consequently the planned container port handling strategies. Developments presented in this work are devoted to a novel design of sliding mode control with low-pass filter (SMC-LPF) to nonlinear handling chain system (HCS) in container ports. The SMC-LPF can effectively reduce unsatisfied freight requirement of the HCS and make chattering decrease significantly. To illustrate the effectiveness and accuracy of the proposed SMC-LPF, an application to a real container port in China is outlined. The performances of the SMC-LPF for the nonlinear HCS in container ports outperform those of the traditional method, particle swarm optimization algorithm, and slide mode control under simulations with a unit step signal and a sinusoidal signal with offset as the freight requirements. The contributions herein demonstrate the proposed control strategy in weakening chattering, reducing the unsatisfied freight requirements to 0 as close as possible in the HCS, maximizing the operation resilience and robustness of port and shipping supply chain against parametric perturbation, external disturbances, and fluctuant handling abilities.
Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1/miR-130a-3p/STAT3 Pathway
Authors: Jiang Xue , Ruan Xue-lei , Xue Yi-xue , Yang Shuang , Shi Mai , Wang Li-ning
Published: February 2020

Senescence of renal tubular epithelial cells plays an important role in diabetic nephropathy, but the mechanism is unknown. Metformin may alleviate diabetic nephropathy by reducing this senescence. This study is aimed at clarifying the effects and mechanism of metformin on the senescence of renal tubular epithelial cells in diabetic nephropathy. We found that metformin reduced the expression of senescence-associated gene P21 in high-glucose-induced (30 mmol/L) renal tubular epithelial cells and decreased the β-galactosidase positive staining rate (decreased 16%, p<0.01). Metformin was able to reduce senescence by upregulating the expression of RNA-binding protein MBNL1 and miR-130a-3p and reducing STAT3 expression. MBNL1 prolonged the half-life of miR-130a-3p, and miR-130a-3p could negatively regulate STAT3 by binding to its mRNA 3′UTR. In db/db diabetic mice, we found an enhanced senescence level combined with low expression of MBNL1 and miR-130a-3p and high expression of STAT3 compared with db/m control mice during nephropathy development. Meanwhile, metformin (200 mg/kg/day) could increase the expression of MBNL1 and miR-130a-3p and decreased STAT3 expression, thus reducing this senescence in db/db mice. Our results suggest that metformin reduces the senescence of renal tubular epithelial cells in diabetic nephropathy via the MBNL1/miR-130a-3p/STAT3 pathway, which provided new ideas for the therapy of this disease.
Simultaneous Ipsilateral Floating Hip and Knee: A Complex Combination and Difficult Surgical Challenge
Authors: Benabbouha Abdellatif , Boussouga Mostapha , Fjouji Salaheddine , Lamkhanter Adil , Jaafar Abdeloihab
Published: February 2020

Simultaneous ipsilateral floating hip and floating knee are extremely rare. To the best of our knowledge, only four cases have been described in the literature. This uncommon injury is mostly caused by high-velocity impact and associated with life-threatening lesions. We report a unique case of concomitant ipsilateral floating hip and floating knee following road traffic accident. The patient presented ipsilateral hip dislocation and acetabular, femoral, and tibial fractures associated with chest trauma. The aim of this report is to highlight the severity and rarity of this combination and to describe the therapeutic recommendations.
The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine
Authors: Zhou Shukui , Wang Ying , Zhang Kaile , Cao Nailong , Yang Ranxing , Huang Jianwen , Zhao Weixin , Rahman Mahbubur , Liao Hong , Fu Qiang
Published: February 2020

To date, the decellularized scaffold has been widely explored as a source of biological scaffolds for regenerative medicine. However, the acellular matrix derived from natural tissues and organs has a lot of defects, including the limited amount of autogenous tissue and surgical complication such as risk of blood loss, wound infection, pain, shock, and functional damage in the donor part of the body. In this study, we prepared acellular matrix using adipose-derived stem cell (ADSC) sheets and evaluate the cellular compatibility and immunoreactivity. The ADSC sheets were fabricated and subsequently decellularized using repeated freeze-thaw, Triton X-100 and SDS decellularization. Oral mucosal epithelial cells were seeded onto the decellularized ADSC sheets to evaluate the cell replantation ability, and silk fibroin was used as the control. Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. The ADSC sheet-derived ECM scaffolds preserved the three-dimensional architecture of ECM and retained the cytokines by Triton X-100 decellularization protocols. Compared with silk fibroin in vitro, the oral mucosal epithelial cells survived better on the decellularized ADSC sheets with an intact and consecutive epidermal cellular layer. Compared with porcine small intestinal submucosa (SIS) in vivo, the homogeneous decellularized ADSC sheets had less monocyte-macrophage infiltrating in vivo implantation. During 3 weeks after transplantation, the mRNA expression of cytokines, such as IL-4/IL-10, was obviously higher in decellularized ADSC sheets than that of porcine SIS. A Triton X-100 method can achieve effective cell removal, retain major ECM components, and preserve the ultrastructure of ADSC sheets. The decellularized ADSC sheets possess good recellularization capacity and excellent biocompatibility. This study demonstrated the potential suitability of utilizing acellular matrix from ADSC sheets for soft tissue regeneration and repair.
Clonal ZEB1-Driven Mesenchymal Transition Promotes Targetable Oncologic Antiangiogenic Therapy Resistance
Authors: Chandra Ankush , Jahangiri Arman , Chen William , Nguyen Alan T. , Yagnik Garima , Pereira Matheus P. , Jain Saket , Garcia Joseph H. , Shah Sumedh S. , Wadhwa Harsh , Joshi Rushikesh S. , Weiss Jacob , Wolf Kayla J. , Lin Jung-Ming G. , Müller Sören , Rick Jonathan W. , Diaz Aaron A. , Gilbert Luke A. , Kumar Sanjay , Aghi Manish K.
Published: February 2020

Glioblastoma (GBM) responses to bevacizumab are invariably transient with acquired resistance. We profiled paired patient specimens and bevacizumab-resistant xenograft models pre- and post-resistance toward the primary goal of identifying regulators whose targeting could prolong the therapeutic window, and the secondary goal of identifying biomarkers of therapeutic window closure. Bevacizumab-resistant patient specimens and xenografts exhibited decreased vessel density and increased hypoxia versus pre-resistance, suggesting that resistance occurs despite effective therapeutic devascularization. Microarray analysis revealed upregulated mesenchymal genes in resistant tumors correlating with bevacizumab treatment duration and causing three changes enabling resistant tumor growth in hypoxia. First, perivascular invasiveness along remaining blood vessels, which co-opts vessels in a VEGF-independent and neoangiogenesis-independent manner, was upregulated in novel biomimetic 3D bioengineered platforms modeling the bevacizumab-resistant microenvironment. Second, tumor-initiating stem cells housed in the perivascular niche close to remaining blood vessels were enriched. Third, metabolic reprogramming assessed through real-time bioenergetic measurement and metabolomics upregulated glycolysis and suppressed oxidative phosphorylation. Single-cell sequencing of bevacizumab-resistant patient GBMs confirmed upregulated mesenchymal genes, particularly glycoprotein YKL-40 and transcription factor ZEB1, in later clones, implicating these changes as treatment-induced. Serum YKL-40 was elevated in bevacizumab-resistant versus bevacizumab-naïve patients. CRISPR and pharmacologic targeting of ZEB1 with honokiol reversed the mesenchymal gene expression and associated stem cell, invasion, and metabolic changes defining resistance. Honokiol caused greater cell death in bevacizumab-resistant than bevacizumab-responsive tumor cells, with surviving cells losing mesenchymal morphology. Employing YKL-40 as a resistance biomarker and ZEB1 as a target to prevent resistance could fulfill the promise of antiangiogenic therapy.

Significance:

Bevacizumab resistance in GBM is associated with mesenchymal/glycolytic shifts involving YKL-40 and ZEB1. Targeting ZEB1 reduces bevacizumab-resistant GBM phenotypes.
Dual Oxidase-Induced Sustained Generation of Hydrogen Peroxide Contributes to Pharmacologic Ascorbate-Induced Cytotoxicity
Authors: Gibson Adrienne R. , O'Leary Brianne R. , Du Juan , Sarsour Ehab H. , Kalen Amanda L. , Wagner Brett A. , Stolwijk Jeffrey M. , Falls-Hubert Kelly C. , Alexander Matthew S. , Carroll Rory S. , Spitz Douglas R. , Buettner Garry R. , Goswami Prabhat C. , Cullen Joseph J.
Published: February 2020

Pharmacologic ascorbate treatment (P-AscH−, high-dose, intravenous vitamin C) results in a transient short-term increase in the flux of hydrogen peroxide that is preferentially cytotoxic to cancer cells versus normal cells. This study examines whether an increase in hydrogen peroxide is sustained posttreatment and potential mechanisms involved in this process. Cellular bioenergetic profiling following treatment with P-AscH− was examined in tumorigenic and nontumorigenic cells. P-AscH− resulted in sustained increases in the rate of cellular oxygen consumption (OCR) and reactive oxygen species (ROS) in tumor cells, with no changes in nontumorigenic cells. Sources for this increase in ROS and OCR were DUOX 1 and 2, which are silenced in pancreatic ductal adenocarcinoma, but upregulated with P-AscH− treatment. An inducible catalase system, to test causality for the role of hydrogen peroxide, reversed the P-AscH−–induced increases in DUOX, whereas DUOX inhibition partially rescued P-AscH−–induced toxicity. In addition, DUOX was significantly downregulated in pancreatic cancer specimens compared with normal pancreas tissues. Together, these results suggest that P-AscH−–induced toxicity may be enhanced by late metabolic shifts in tumor cells, resulting in a feed-forward mechanism for generation of hydrogen peroxide and induction of metabolic stress through enhanced DUOX expression and rate of oxygen consumption.

Significance:

A high dose of vitamin C, in addition to delivering an acute exposure of H2O2 to tumor cells, activates DUOX in pancreatic cancer cells, which provide sustained production of H2O2.
Extensive Clonal Branching Shapes the Evolutionary History of High-Risk Pediatric Cancers
Authors: Andersson Natalie , Bakker Bjorn , Karlsson Jenny , Valind Anders , Holmquist Mengelbier Linda , Spierings Diana C.J. , Foijer Floris , Gisselsson David
Published: February 2020

Darwinian evolution of tumor cells remains underexplored in childhood cancer. We here reconstruct the evolutionary histories of 56 pediatric primary tumors, including 24 neuroblastomas, 24 Wilms tumors, and 8 rhabdomyosarcomas. Whole-genome copy-number and whole-exome mutational profiling of multiple regions per tumor were performed, followed by clonal deconvolution to reconstruct a phylogenetic tree for each tumor. Overall, 88% of the tumors exhibited genetic variation among primary tumor regions. This variability typically emerged through collateral phylogenetic branching, leading to spatial variability in the distribution of more than 50% (96/173) of detected diagnostically informative genetic aberrations. Single-cell sequencing of 547 individual cancer cells from eight solid pediatric tumors confirmed branching evolution to be a fundamental underlying principle of genetic variation in all cases. Strikingly, cell-to-cell genetic diversity was almost twice as high in aggressive compared with clinically favorable tumors (median Simpson index of diversity 0.45 vs. 0.88; P = 0.029). Similarly, a comparison of multiregional sampling data from a total of 274 tumor regions showed that new phylogenetic branches emerge at a higher frequency per sample and carry a higher mutational load in high-risk than in low-risk tumors. Timelines based on spatial genetic variation showed that the mutations most influencing relapse risk occur at initiation of clonal expansion in neuroblastoma and rhabdomyosarcoma, whereas in Wilms tumor, they are late events. Thus, from an evolutionary standpoint, some high-risk childhood cancers are born bad, whereas others grow worse over time.

Significance:

Different pediatric cancers with a high risk of relapse share a common generic pattern of extensively branching evolution of somatic mutations.
A Noncanonical Role of Fructose-1, 6-Bisphosphatase 1 Is Essential for Inhibition of Notch1 in Breast Cancer
Authors: Lu Chao , Ren Chune , Yang Tingting , Sun Yonghong , Qiao Pengyun , Wang Dan , Lv Shijun , Yu Zhenhai
Published: February 2020

Breast cancer is a leading cause of death in women worldwide, but the underlying mechanisms of breast tumorigenesis remain unclear. Fructose-1, 6-bisphosphatase 1 (FBP1), a rate-limiting enzyme in gluconeogenesis, was recently shown to be a tumor suppressor in breast cancer. However, the mechanisms of FBP1 as a tumor suppressor in breast cancer remain to be explored. Here we showed that FBP1 bound to Notch1 in breast cancer cells. Moreover, FBP1 enhanced ubiquitination of Notch1, further leading to proteasomal degradation via FBXW7 pathway. In addition, we found that FBP1 significantly repressed the transactivation of Notch1 in breast cancer cells. Functionally, Notch1 was involved in FBP1-mediated tumorigenesis of breast cancer cells in vivo and in vitro. Totally, these findings indicate that FBP1 inhibits breast tumorigenesis by regulating Notch1 pathway, highlighting FBP1 as a potential therapeutic target for breast cancer.

Implications:

We demonstrate FBP1 as a novel regulator for Notch1 in breast cancer.
Call to Action: Rural Health: A Presidential Advisory From the American Heart Association and American Stroke Association
Authors: Harrington Robert A. , Califf Robert M. , Balamurugan Appathurai , Brown Nancy , Benjamin Regina M. , Braund Wendy E. , Hipp Janie , Konig Madeleine , Sanchez Eduardo , Joynt Maddox Karen E.
Published: February 2020

Understanding and addressing the unique health needs of people residing in rural America is critical to the American Heart Association’s pursuit of a world with longer, healthier lives. Improving the health of rural populations is consistent with the American Heart Association’s commitment to health equity and its focus on social determinants of health to reduce and ideally to eliminate health disparities. This presidential advisory serves as a call to action for the American Heart Association and other stakeholders to make rural populations a priority in programming, research, and policy. This advisory first summarizes existing data on rural populations, communities, and health outcomes; explores 3 major groups of factors underlying urban-rural disparities in health outcomes, including individual factors, social determinants of health, and health delivery system factors; and then proposes a set of solutions spanning health system innovation, policy, and research aimed at improving rural health.
A healthy sports club initiative in action in Ireland
Authors: Lane Aoife , Murphy Niamh , Donohoe Alex , Regan Colin
Published: February 2020

Objective:

The Gaelic Athletic Association (GAA) is part of the cultural fabric of Irish society with a club in almost every community nationwide. The aim of this project was to carry out a pilot evaluation of the GAA Healthy Club Project (HCP), which is a unique effort by a national governing body to include health as part of the core business of the organisation at grass roots level.

Design:

A pre–post intervention group only design was used across 18 clubs recruited to a pilot phase of the project.

Setting:

GAA grassroots sports clubs across Ireland.

Methods:

Twelve Healthy Club Officers completed a self-evaluation survey of their club at two time points to indicate the health promotion orientation of their club and the extent of health promotion activity in the club.

Results:

Data showed improvements in the health promotion orientation of clubs, from moderate to high health promoting overall and particular increases in policy and practice scores. This is likely due to the widespread appointment of Healthy Club Officers and the delivery of health-related initiatives in clubs.

Conclusion:

The impact of the project, while not demonstrable as an intervention effect at this stage, was real for the clubs involved. The GAA HCP is a novel way of carrying out health promotion in Ireland, serving as a meeting point between the ‘push of health’ and ‘pull of the sports club’.
Transcending dichotomies: Informal work, young people and the state in Argentina
Authors: Longo María Eugenia
Published: February 2020

Since the beginning of modern labor markets, formal and informal activities have coexisted alongside each other. However, most of the traditional approaches to informality are not fully able to explain the multiple forms by which this phenomenon manifests itself today in certain contexts. Informality in Latin American societies, particularly in Argentina, is heuristically revelatory for illustrating the need for a more complex definition and less rigid theoretical classifications. The main purpose of this article is to contribute to an empirical analysis about informality by addressing two points. First, the article provides a better understanding of the heterogeneous nature of informality—“involuntary” or “voluntary” informal work, “half-formal/half-informal” work—in the field of youth employment. Second, in connection with debates about the sources of informality, the article explores the hypothesis of the existence of state-created informality. The article illustrates these points at different levels: youth careers, employers’ recruitment strategies, and states policies. This perspective seems useful for identifying the actors involved, acknowledging the blurry boundaries between diverse informal situations, and understanding actors’ uses of them. The points are illustrated by evidence from a qualitative and longitudinal study on youth employment careers in Argentina. The article concludes with a discussion about some key issues of informality, such as legality, regulation and agency.
Setting, Age, and Intensity Influence Responses to Exercise in Young Endurance Runners
Authors: Reich Alexander H. , Queathem Elizabeth J.
Published: February 2020

We investigated the effects of exercise setting (indoor treadmill vs. outdoor trail), age (17 middle school, 18 high school, and 13 college participants), and level of exertion (Borg Ratings of Perceived Exertion of 10, 12, and 16 on a 22-point scale) on young male endurance runners’ heart rate (HR), running speed, attentive focus, and affect. Three-way analyses of variance revealed that on the outdoor trail (vs. indoor treadmill), HR and speed were higher ( p < .001) and attentive focus was more dissociative ( p = .047). There were significant Age × Setting interactions for HR ( p = .047), speed ( p = .023), and attentive focus ( p = .002), with older participants exhibiting a greater increase in speed and HR and a greater shift toward dissociative focus on the outdoor trail. Three-way analyses of variance also yielded significant Age × Time interactions on components of the Physical Activity Affect Scale in that younger participants exhibited larger declines in positive affect ( p = .003) and tranquility ( p < .001) and larger increases in fatigue ( p < .001) as a result of the running session. Our data suggest that either runners develop more positive responses to exercise as they mature or those young runners who experience running more negatively tend to drop out of running so that the remaining older participants continuing to run are those who experience exercise positively.
Social cohesion without electoral democracy: The case of China
Authors: Pei Wang , Bell Daniel A.
Published: February 2020

Democratic elections, whatever the flaws, tend to produce a sense of social cohesion as ordinary citizens, treated as equals, gather together to select their country’s political leaders. In China, leaders are not selected by means of democratic elections. How can China secure social cohesion without national elections? We will discuss some responses from the government and from scholars and ‘ordinary people’.
Dynamic pulse buckling and failure analysis of the single curved composite sandwich panel using the core high-order theory
Authors: Ahmadi Seyed Ali , Pashaei Mohammad Hadi , Jafari-Talookolaei Ramazan-Ali
Published: February 2020

The current study aims to investigate the facesheet dynamic pulse buckling of simply supported, cylindrical composite sandwich panels using the Budiansky–Roth buckling criterion. The foam core has been modeled with isotropic elastic-perfectly plastic properties and various failure modes of the sandwich panel like facesheet fracture, foam shear fracture, and foam yield are investigated. The extended high-order sandwich panel core theory was used to model the compressibility of the core. To study the mechanical properties of the viscoelastic foam core, the Kelvin–Voigt linear viscoelastic model was applied. The transient responses and stress components obtained from the present method are compared with finite element solutions using commercial software ANSYS and those reported in the literature. Accordingly, reasonable agreement is observed. It was shown that the pulse local buckling strength of the panel increases with a decrease in the panel radius or an increase in the thickness of the panel, and facesheet fracture is considered more a likely failure mode of these sandwich panels.
Design improvement and fatigue analysis for a bicycle handlebar stem system using uniform design method and genetic algorithm
Authors: Jiang Cho-Pei , Wu Ching-Wei , Cheng Yung-Chang
Published: February 2020

An integrating optimization procedure is presented to improve the von Mises stress and fatigue safety factor for a handlebar stem system in a bicycle system. The optimization procedure involves uniform design of experiment, Kriging interpolation, genetic algorithm, and nonlinear programming method. Using ANSYS/Workbench software and the ISO 4210 bicycle handlebar stem testing standard, the von Mises stress for the lateral bending test simulation and the fatigue safety factor for the fatigue test simulation is calculated. The von Mises stress and fatigue safety factor are combined into a single and integrated objective function, and Kriging interpolation is then used to create the surrogate model of the integrated objective function. When the integrating optimization procedure is used, the integrated objective function demonstrates that the von Mises stress for the optimized handlebar stem is reduced to 225 MPa and the fatigue safety factor increases to 1.796. This shows that the optimized design increases the strength of the handlebar stem. The proposed technique yields a handlebar stem with an optimized shape.
Holocene vegetation history in the Northern Vosges Mountains (NE France): Palynological, geochemical and sedimentological data
Authors: Gouriveau Emilie , Ruffaldi Pascale , Duchamp Loïc , Robin Vincent , Schnitzler Annik , Walter-Simonnet Anne-Véronique
Published: February 2020

Palynological data from the Northern Vosges Mountains (NVM) are very rare, unlike for the Southern and Central Vosges Mountains, where the past vegetation history is relatively well known. As a consequence, the beginning of human activities has never been clearly identified and dated in the NVM. In order to reconstruct the evolution of vegetation in this region, multiproxy studies (pollen, non-pollen palynomorphs, sedimentological and geochemical analyses) were conducted in two peatlands. Overall, the results, extending from about 9500 cal. BP to recent times, show a classical vegetation succession with local particularities resulting from human activities. In the La Horn peatland, a strong human impact related to pastoralism is attested from the late Bronze Age onwards. The second phase of human occupation, mainly characterized by crop cultures, begins during the Hallstatt period. The geochemical results (x-ray fluorescence) also highlight the presence of metallic elements, which, combined with significant quantities of carbonized particles, point to potential metal working. In the Kobert-Haut peatland, human occupation began much later (1500 cal. BP), but lasted from the Gallo-Roman period to the beginning of the Modern Period. Unlike for the vegetation history of the rest of the Vosges, Pinus remains a prevailing taxon throughout the Holocene in the NVM. Another particularity is the early establishment of Picea, long before the 18th to 19th century plantations.
Test–Retest Reliability and Temporal Agreement of Direct and Indirect Sexual Interest Measures
Authors: Welsch Robin , Schmidt Alexander F. , Turner Daniel , Rettenberger Martin
Published: February 2020

The Explicit and Implicit Sexual Interest Profile (EISIP) is a multimethod measure of sexual interest in children and adults. It combines indirect latency-based measures such as the Implicit Association Test (IAT), Viewing Time (VT), and explicit self-report measures. This study examined test–retest reliability and absolute temporal agreement of the EISIP over a 2-week interval in persons who were convicted of sexual offenses against children ( n = 33) and nonoffending controls ( n = 48). Test–retest reliability of the aggregated EISIP measures was high across the whole sample ( rtt = .90, intraclass correlation coefficient [ICC] = .90) with the IAT yielding the lowest retest correlations ( rtt = .66, ICC = .66). However, these indicators of relative reliability only quantify the temporal stability of individual differences within the group, not the detectability of individual change. Absolute temporal agreement as assessed via Bland–Altman plots ranged from one fourth to three thirds of a standardized unit in the sexual preference scores. This implies that individual change has to exceed medium to large standardized effect sizes to be distinguishable from spontaneous temporal variation in the EISIP measures. Overall, scores of combined measures were largely superior to single measures in terms of both absolute and relative reliability.
Learning to Do Qualitative Data Analysis: A Starting Point
Authors: Lester Jessica Nina , Cho Yonjoo , Lochmiller Chad R.
Published: February 2020

Given the vast and diverse qualitative analytic landscape, what might be a generative starting point for researchers who desire to learn how to produce quality qualitative analyses? This question is particularly relevant to researchers new to the field and practice of qualitative research and instructors and mentors who regularly introduce students to qualitative research practices. In this article, we seek to offer what we view as a useful starting point for learning how to do qualitative analysis. We begin by discussing briefly the general landscape of qualitative research methodologies and methods. To contextualize our suggestions, we review the qualitative analytic practices commonly used within human resource development (HRD). Following this, we describe thematic analysis in more detail, including why we believe it is a particularly useful analytic approach to consider when first learning about qualitative analysis. We share seven common practices or important considerations for carrying out a thematic analysis and conclude by highlighting key considerations for assuring quality when conducting a thematic analysis.
Frequency, Pattern, and Treatment of Hand Fractures in Children and Adolescents: A 27-Year Review of 4356 Pediatric Hand Fractures
Authors: Kreutz-Rodrigues Lucas , Gibreel Waleed , Moran Steven L. , Carlsen Brian T. , Bakri Karim
Published: February 2020

Background: The frequency, pattern, and treatment of pediatric hand fractures are rarely reported. We sought to review our institution’s experience in the management of pediatric hand fractures. Methods: A retrospective review of children and adolescents (younger than 18 years) treated for hand fractures between January 1990 and June 2017 was preformed. Fractures were categorized into metacarpal, proximal/middle phalanx, distal phalanx, or intra-articular metacarpophalangeal (MCP)/proximal interphalangeal (PIP)/distal interphalangeal (DIP) fractures. Patients were categorized into 3 age groups (0-5, 6-11, and 12-17 years). Results: A total of 4356 patients were treated for hand fractures at a mean ± SD age of 12.2 ± 3.5 years. Most fractures occurred in patients aged 12 to 17 years (n = 2775, 64%), followed by patients aged 6 to 11 years (n = 1347, 31%). Only 234 (5%) fractures occurred in children younger than 5 years. Most fractures occurred in the proximal/middle phalanx (48%), followed by metacarpal (33%), distal phalangeal (12%), and intra-articular MCP/PIP/DIP joints (7%). Proximal/middle phalangeal fractures were the most common in all age groups. About 58% of intra-articular MCP/PIP/DIP fractures in patients aged between 0 and 5 years required open reduction ± fixation, and the remaining 42% fractures were amenable to closed reduction. In patients older than 5 years, about 70% of these fractures were amenable to closed reduction. All age groups included, most metacarpal (93%), proximal/middle phalangeal (92%), and distal phalangeal (86%) fractures were amenable to closed reduction alone. Conclusions: The frequency, pattern, and treatment of hand fractures vary among different age groups. Understanding the pattern of these fractures helps making the right diagnosis and guides choosing the appropriate treatment.
Spontaneous spinal epidural haematoma following intra-arterial catheter-directed thrombolysis: A case report
Authors: Shannon Nicholas Brian , Kumar Pradesh , Tay Kiang Hiong , Tan Sia Yang , Chng Siew Ping , Tay Hsien Tsung
Published: February 2020

A 79-year-old Chinese gentleman presented with unilateral acute lower limb ischaemia and received intra-arterial catheter-directed thrombolysis, initially with good result and reversal of the ischaemia. However, he developed an extensive spontaneous spinal epidural haematoma within hours of the procedure and was left with permanent paraplegia after being deemed unsuitable for decompressive spinal surgery. This report serves as a reminder of the risk of severe complications of catheter-directed thrombolysis by describing this rare but devastating side-effect that occurred even despite early detection from onset of symptoms.
Smectitic clays enriched with ferric ions for the rapid removal of anionic dyes in aqueous media
Authors: Rodrigues Itamar A. , Villalba Juan C. , Santos Maria J. , Melquiades Fábio L. , Anaissi Fauze J.
Published: February 2020

Sodium smectite clays were enriched with ferric ions (Argel-Fe and Volclay-Fe) to convert the surface charge of the clays from negative to positive and to use the clays in the discolouration of a synthetic effluent composed of seven anionic dyes (mixed from tartrazine, Brilliant Blue FCF and amaranth). The iron content increased from 5.99% to 11.02% for Argel-Fe and from 5.39% to 10.54% for Volclay-Fe. The efficiency of the discolouration of the anion dye mixture was evaluated by measuring the absorbance of the mixture at 562 nm, where the band with the greatest intensity was found. The contact time required for the system to reach equilibrium was ~5 min for both adsorbents. The kinetic adsorption data supported a pseudo-second-order kinetic model. The experimental data support the dual-site Langmuir–Freundlich isotherm model. The maximum adsorption capacities were 88.68 mg g–1for Argel-Fe and 392.21 mg g–1for Volclay-Fe. The enrichment of clays with Fe(III) added functionality to the clays and generated adsorbents with rapid adsorption abilities and high discolouration capacities.
Dual dependence on BCL2 and MCL1 in T-cell prolymphocytic leukemia
Authors: Smith Victoria M. , Lomas Oliver , Constantine Donna , Palmer Lianne , Schuh Anna H. , Bruce David , Gonchar Oksana , Macip Salvador , Jayne Sandrine , Dyer Martin J. S. , Eyre Toby A.
Published: February 2020

Key Points

Treatment of relapsed refractory T-PLL with venetoclax monotherapy results in only transient and minor clinical responses. In vitro analyses pre- and postvenetoclax indicate dual dependence on BCL2 and MCL1; combined BCL2 and MCL1 inhibition are synergistic.
Gilteritinib is a clinically active FLT3 inhibitor with broad activity against FLT3 kinase domain mutations
Authors: Tarver Theodore C. , Hill Jason E. , Rahmat Leena , Perl Alexander E. , Bahceci Erkut , Mori Kenichi , Smith Catherine C.
Published: February 2020

Gilteritinib is the first FMS-like tyrosine kinase 3 (FLT3) tyrosine kinase inhibitor (TKI) approved as monotherapy in acute myeloid leukemia with FLT3 internal tandem duplication and D835/I836 tyrosine kinase domain (TKD) mutations. Sequencing studies in patients have uncovered less common, noncanonical (NC) mutations in FLT3 and have implicated secondary TKD mutations in FLT3 TKI resistance. We report that gilteritinib is active against FLT3 NC and TKI resistance-causing mutations in vitro. A mutagenesis screen identified FLT3 F691L, Y693C/N, and G697S as mutations that confer moderate resistance to gilteritinib in vitro. Analysis of patients treated with gilteritinib revealed that 2/9 patients with preexisting NC FLT3 mutations responded and that secondary TKD mutations are acquired in a minority (5/31) of patients treated with gilteritinib. Four of 5 patients developed F691L mutations (all treated at <200 mg). These studies suggest that gilteritinib has broad activity against FLT3 mutations and limited vulnerability to resistance-causing FLT3 TKD mutations, particularly when used at higher doses.
Effect of short-term inhalation of warm saline atomised gas on patients with non-cystic fibrosis bronchiectasis
Authors: Zhong Lihong , Xiong Ying , Zheng Zeguang , Liu Ni , Hu Jieying , Yang Feng , Chen Rongchang
Published: February 2020

Objective

Secretion clearance is advocated in non-cystic fibrosis bronchiectasis, but is often neglected in clinical treatment. The present study aimed to investigate the effect of inhaled 0.9% normal saline by ultrasonic nebuliser with warming (UNW) in stable non-cystic fibrosis bronchiectasis patients with purulent sputum.

Methods

27 stable patients were enrolled in a randomised crossover trial comprising 3 months of daily UNW aerosol therapy compared with 3 months of daily oral expectorant treatment. The outcomes were quality of life (assessedviathe Leicester cough questionnaire (LCQ)), sputum characteristics, pulmonary function, 6-min walk distance (6MWD) and acute exacerbation frequency.

Results

Compared with baseline, the sputum viscosity, sputum colour, LCQ score and 6MWD were significantly improved with UNW (p<0.05), and the improvements in sputum colour, LCQ score, 6MWD and inspirational capacity with UNW were significantly better than those achievedviatreatment with oral expectorant (p<0.05). There were no significant differences between treatments in pulmonary function, sputum cytology classification and inflammatory factors of sputum.

Conclusion

UNW aerosol therapy in non-cystic fibrosis bronchiectasis has small yet significant benefits. However, UNW does not improve the degree of inflammation.
Routine neck ultrasound by respiratory physicians in the diagnosis and staging of patients with lung cancer and mediastinal lymphadenopathy: a prospective pilot study
Authors: Ahmed Mohammed , Daneshvar Cyrus , Breen David
Published: February 2020

Introduction

Cervical lymphadenopathy in lung cancer indicates advanced disease. The presence of mediastinal lymphadenopathy is commonly associated with involvement of neck lymph nodes and some studies suggest routine neck ultrasound (NUS) in this group of patients. We conducted a two-phase study looking at training a respiratory physician to perform ultrasound-guided neck lymph node aspiration in patients with suspected lung cancer.

Methods

In the first phase of the study, one of the authors underwent training in NUS according to predetermined criteria. The adequacy of sampling was prospectively recorded. In the second phase, consecutive patients with suspected lung cancer and mediastinal lymphadenopathy underwent NUS and sampling of abnormal lymph nodes. The outcomes were the adequacy of samples for pathological analysis and molecular analysis, prevalence of cervical lymphadenopathy, and change in stage.

Results

Following the period of training, 35 patients underwent neck node sampling with an overall adequacy of 88.6% (95% CI 78.1–99.1%). Cervical lymph node involvement was confirmed in 13 out of 30 patients with lung cancer (43.3%, 95% CI 25.5–62.6%). Further immunohistochemistry and molecular studies were possible in all patients when it was required (nine cases). NUS led to nodal upstaging in four out of 30 (13.3%) cases.

Conclusion

Training a respiratory physician to perform NUS and needle sampling to an acceptable level is feasible. Benefits of embedding this procedure in lung cancer diagnosis and pathway staging need to be explored in further studies.