bluuebunny/crossref_metadata_2025 huggingface dataset:
Vector Products As A Tool For The Investigation Of The Isometric Transformation Of Objects
Authors: Grabowski Ryszard Józef
Published: August 2015

The identification of isometric displacements of studied objects with utilization of the vector product is the aim of the analysis conducted in this paper. Isometric transformations involve translation and rotation. The behaviour of distances between check points on the object in the first and second measurements is a necessary condition for the determination of such displacements. For every three check points about the measured coordinate, one can determine the vector orthogonal to the two neighbouring sides of the triangle that are treated as vectors, using the definition of the vector product in three-dimensional space. If vectors for these points in the first and second measurements are parallel to the studied object has not changed its position or experienced translation. If the termini of vectors formed from vector products treated as the vectors are orthogonal to certain axis, then the object has experienced rotation. The determination of planes symmetric to these vectors allows the axis of rotation of the object and the angle of rotation to be found. The changes of the value of the angle between the normal vectors obtained from the first and second measurements, by exclusion of the isometric transformation, are connected to the size of the changes of the coordinates of check points, that is, deformation of the object. This paper focuses mainly on the description of the procedure for determining the translation and rotation. The main attention was paid to the rotation, due to the new and unusual way in which it is determined. Mean errors of the determined parameters are often treated briefly, and this subject requires separate consideration.
Molecular cancer prevention: Current status and future directions
Authors: Maresso Karen Colbert , Tsai Kenneth Y. , Brown Powel H. , Szabo Eva , Lippman Scott , Hawk Ernest T.
Published: August 2015

Answer questions and earn CME/CNE

The heterogeneity and complexity of advanced cancers strongly support the rationale for an enhanced focus on molecular prevention as a priority strategy to reduce the burden of cancer. Molecular prevention encompasses traditional chemopreventive agents as well as vaccinations and therapeutic approaches to cancer‐predisposing conditions. Despite challenges to the field, we now have refined insights into cancer etiology and early pathogenesis; successful risk assessment and new risk models; agents with broad preventive efficacy (eg, aspirin) in common chronic diseases, including cancer; and a successful track record of more than 10 agents approved by the US Food and Drug Administration for the treatment of precancerous lesions or cancer risk reduction. The development of molecular preventive agents does not differ significantly from the development of therapies for advanced cancers, yet it has unique challenges and special considerations given that it most often involves healthy or asymptomatic individuals. Agents, biomarkers, cohorts, overall design, and endpoints are key determinants of molecular preventive trials, as with therapeutic trials, although distinctions exist for each within the preventive setting. Progress in the development and evolution of molecular preventive agents has been steadier in some organ systems, such as breast and skin, than in others. In order for molecular prevention to be fully realized as an effective strategy, several challenges to the field must be addressed. Here, the authors provide a brief overview of the context for and special considerations of molecular prevention along with a discussion of the results from major randomized controlled trials. CA Cancer J Clin 2015;65:345–383. © 2015 American Cancer Society.
RandAgiamo™, a Pilot Project Increasing Adoptability of Shelter Dogs in the Umbria Region (Italy)
Authors: Menchetti Laura , Mancini Stefania , Catalani Maria , Boccini Beatrice , Diverio Silvana
Published: August 2015

Current Italian legislation does not permit euthanasia of dogs, unless they are ill or dangerous. Despite good intentions and ethical benefits, this ‘no-kill policy’ has caused a progressive overpopulation of dogs in shelters, due to abandonment rates being higher than adoption rates. Shelter overcrowding has negative implications for dog welfare and increases public costs. The aim of this paper is to describe the pilot project “RandAgiamo” implemented in a rescue shelter in the Umbria Region and to evaluate its effectiveness on the rate of dog adoption using official data. RandAgiamo aimed to increase adult shelter dogs’ adoptability by a standard training and socialization programme. It also promoted dogs’ visibility by publicizing them through social media and participation in events. We analysed the official data of the Umbria regional health authorities regarding dog shelters of the Perugia province of the year 2014. In the RandAgiamo shelter, the dog adoption rate was 27.5% higher than that of dogs housed in other shelters located in the same geographical area (P < 0.001). The RandAgiamo project could be beneficial for the dogs’ welfare, owner satisfaction, shelter management, and public perception of shelter dogs. However, staff were required to provide dog training and related activities.
B Cell Epitope-Based Vaccination Therapy
Authors: Kametani Yoshie , Miyamoto Asuka , Tsuda Banri , Tokuda Yutaka
Published: August 2015

Currently, many peptide vaccines are undergoing clinical studies. Most of these vaccines were developed to activate cytotoxic T cells; however, the response is not robust. Unlike vaccines, anti-cancer antibodies based on passive immunity have been approved as a standard treatment. Since passive immunity is more effective in tumor treatment, the evidence suggests that limited B cell epitope-based peptide vaccines may have similar activity. Nevertheless, such peptide vaccines have not been intensively developed primarily because humoral immunity is thought to be preferable to cancer progression. B cells secrete cytokines, which suppress immune functions. This review discusses the possibility of therapeutic antibody induction by a peptide vaccine and the role of active and passive B cell immunity in cancer patients. We also discuss the use of humanized mice as a pre-clinical model. The necessity of a better understanding of the activity of B cells in cancer is also discussed.
An Investigation of the Thermal Expansion Coefficient for Resin Concrete with ZrW2O8
Authors: Lin Kuangzhe , Qiu Shuang , Lin Bin , Wang Yuguo
Published: August 2015

This paper presents a novel resin concrete obtained by adding cubic zirconium tungstate (ZrW2O8) as filler. A prediction algorithm on the thermal expansion coefficient (CTE) of resin concrete (including filler) was established on the basis of the meso-mechanics method and a three-phase model for concrete. The concept of twice mixing was also proposed for prediction accuracy. Then, a 2D and 3D irregular polygon aggregate particles packing model was set up by Matlab and the properties of the packing model were simulated by finite element analysis. Finally, resin concrete samples were made and their CTE were measured. Mix proportion and addition of ZrW2O8 as influencing factors were considered in this experiment. The CTE of resin concrete was verified by comparing results of the prediction model, simulation model and experiment. The optimum CTE obtained from the experiment was 1.504 × 10−6/K. Compared with 6.817 × 10−6/K without ZrW2O8, it was found that the addition of ZrW2O8 to resin concrete can make it perform significantly better in thermal expansion.
New Programmable CMOS Fuzzifier and C2V Circuits Applicable in FLC Chip for Signal Processing of MEMS Glucose Sensors
Authors: Yosefi Ghader , Mirzakuchaki Sattar , Raissi Farshid
Published: August 2015

This paper presents the design and simulation of improved circuits of Fuzzifier and capacitance to voltage (C2V) converter. The Fuzzifier circuit is designed based on analog advantages such as low die area, high accuracy, and simplicity which are added to the fuzzy system advantages. For implementing this idea, a programmable Membership Function Generator (MFG) including differential pair circuit as a Fuzzifier is proposed. The MFG generates arbitrary forms of Gaussian, Trapezoidal, and Triangular shapes. The shape types are achieved using control switches and different reference voltages. This structure is also general purpose in tuning the slope of Membership Functions (MFs) using scaled transistors with different W/L ratios. With a specific purpose in mind, we used it here to generate fuzzy language terms from sensed classic data of a blood glucose microsensor. Thus we proposed a C2V circuit to convert capacitance variations (from MEMS glucose microsensor) to voltage values as classic data. The proposed mentioned circuits can be applicable in design of Fuzzy Logic Controller (FLC) chips to detect blood glucose, process its data in Fuzzy environment, and control insulin injection of diabetic patients by MEMS micropumps. The simulation results are achieved by MATLAB and Hspice software in 0.35 μm CMOS standard technology.
Macrophage Expression of Inflammatory Genes in Response to EMCV Infection
Authors: Shaheen Zachary , Corbett John
Published: August 2015

The expression and production of type 1 interferon is the classic cellular response to virus infection. In addition to this antiviral response, virus infection also stimulates the production of proinflammatory mediators. In this review, the pathways controlling the induction of inflammatory genes and the roles that these inflammatory mediators contribute to host defense against viral pathogens will be discussed. Specific focus will be on the role of the chemokine receptor CCR5, as a signaling receptor controlling the activation of pathways leading to virus-induced inflammatory gene expression.
Ultraviolet Radiation-Induced Cytogenetic Damage in White, Hispanic and Black Skin Melanocytes: A Risk for Cutaneous Melanoma
Authors: Dasgupta Amrita , Katdare Meena
Published: August 2015

Cutaneous Melanoma (CM) is a leading cause of cancer deaths, with reports indicating a rising trend in the incidence rate of melanoma among Hispanics in certain U.S. states. The level of melanin pigmentation in the skin is suggested to render photoprotection from the DNA-damaging effects of Ultraviolet Radiation (UVR). UVR-induced DNA damage leads to cytogenetic defects visualized as the formation of micronuclei, multinuclei and polymorphic nuclei in cells, and a hallmark of cancer risk. The causative relationship between Sun exposure and CM is controversial, especially in Hispanics and needs further evaluation. This study was initiated with melanocytes from White, Hispanic and Black neonatal foreskins which were exposed to UVR to assess their susceptibility to UVR-induced modulation of cellular growth, cytogenetic damage, intracellular and released melanin. Our results show that White and Hispanic skin melanocytes with similar levels of constitutive melanin are susceptible to UVR-induced cytogenetic damage, whereas Black skin melanocytes are not. Our data suggest that the risk of developing UVR-induced CM in a skin type is correlated with the level of cutaneous pigmentation and its ethnic background. This study provides a benchmark for further investigation on the damaging effects of UVR as risk for CM in Hispanics.
Peri-Operative Management of Older Adults with Cancer—The Roles of the Surgeon and Geriatrician
Authors: Parks Ruth , Rostoft Siri , Ommundsen Nina , Cheung Kwok-Leung
Published: August 2015

Optimal surgical management of older adults with cancer starts pre-operatively. The surgeon plays a key role in the appropriate selection of patients and procedures, optimisation of their functional status prior to surgery, and provision of more intensive care for those who are at high risk of post-operative complications. The literature, mainly based on retrospective, non-randomised studies, suggests that factors such as age, co-morbidities, pre-operative cognitive function and intensity of the surgical procedure all appear to contribute to the development of post-operative complications. Several studies have shown that a pre-operative geriatric assessment predicts post-operative mortality and morbidity as well as survival in older surgical cancer patients. Geriatricians are used to working in multidisciplinary teams that assess older patients and make individual treatment plans. However, the role of the geriatrician in the surgical oncology setting is not well established. A geriatrician could be a valuable contribution to the treatment team both in the pre-operative stage (patient assessment and pre-operative optimisation) and the post-operative stage (patient assessment and treatment of medical complications as well as discharge planning).
Portable EDXRF for Quality Assurance of Cosmetics
Authors: Melquiades Fábio , Parreira Paulo , Endo Lucas , Santos Geiele , Wouk Luana , Filho Otávio
Published: August 2015

Portable Energy Dispersive X-ray Fluorescence is a viable, cost and time effective analytical technique for qualitative and quantitative evaluation of a wide range of samples. The objective of this study is to present a methodology for quantification of nail polishes, eye shadows, lipsticks and lip gloss using thin film geometry. The samples were applied over thin films, simulating its use on face and nails. It was possible to quantify S, K, Ca, Ti, Mn, Fe, Cu, Zn, Br, Rb, Sr, Ba and Bi. The methodology is viable and could be useful to forensic science, quality control on industry of raw materials or final products and supervision by regulatory agencies.
Parametric Analysis of a Two-Shaft Aeroderivate Gas Turbine of 11.86 MW
Authors: Lugo-Leyte R. , Salazar-Pereyra M. , Méndez H. , Aguilar-Adaya I. , Ambriz-García J. , Vargas J.
Published: August 2015

The aeroderivate gas turbines are widely used for power generation in the oil and gas industry. In offshore marine platforms, the aeroderivative gas turbines provide the energy required to drive mechanically compressors, pumps and electric generators. Therefore, the study of the performance of aeroderivate gas turbines based on a parametric analysis is relevant to carry out a diagnostic of the engine, which can lead to operational as well as predictive and/or corrective maintenance actions. This work presents a methodology based on the exergetic analysis to estimate the irrevesibilities and exergetic efficiencies of the main components of a two-shaft aeroderivate gas turbine. The studied engine is the Solar Turbine Mars 100, which is rated to provide 11.86 MW. In this engine, the air is compressed in an axial compressor achieving a pressure ratio of 17.7 relative to ambient conditions and a high pressure turbine inlet temperature of 1220 °C. Even if the thermal efficiency associated to the pressure ratio of 17.7 is 1% lower than the maximum thermal efficiency, the irreversibilities related to this pressure ratio decrease approximately 1 GW with respect to irreversibilities of the optimal pressure ratio for the thermal efficiency. In addition, this paper contributes to develop a mathematical model to estimate the high turbine inlet temperature as well as the pressure ratio of the low and high pressure turbines.
A Model for Scale-Free Networks: Application to Twitter
Authors: Aparicio Sofía , Villazón-Terrazas Javier , Álvarez Gonzalo
Published: August 2015

In the last few years, complex networks have become an increasingly relevant research topic due to the large number of fields of application. Particularly, complex networks are especially significant in the area of modern online social networks (OSNs). OSNs are actually a challenge for complex network analysis, as they present some characteristics that hinder topology processing. Concretely, social networks’ volume is exceedingly big, as they have a high number of nodes and links. One of the most popular and influential OSNs is Twitter. In this paper, we present a model to describe the growth of scale-free networks. This model is applied to Twitter after checking that it can be considered a “scale-free” complex network fulfilling the small world property. Checking this property involves the calculation of the shortest path between any two nodes of the network. Given the difficulty of this computation for large networks, a new heuristic method is also proposed to find the upper bounds of the path lengths instead of computing the exact length.
Space Charge Behavior in Paper Insulation Induced by Copper Sulfide in High-Voltage Direct Current Power Transformers
Authors: Liao Ruijin , Hu Ende , Yang Lijun , Yuan Yuan
Published: August 2015

The main insulation system in high-voltage direct current (HVDC) transformer consists of oil-paper insulation. The formation of space charge in insulation paper is crucial for the dielectric strength. Unfortunately, space charge behavior changes because of the corrosive sulfur substance in oil. This paper presents the space charge behavior in insulation paper induced by copper sulfide generated by corrosive sulfur in insulation oil. Thermal aging tests of paper-wrapped copper strip called the pigtail model were conducted at 130 °C in laboratory. Scanning electron microscopy (SEM) was used to observe the surface of copper and paper. Pulse electroacoustic (PEA) and thermally stimulated current (TSC) methods were used to obtain the space charge behavior in paper. Results showed that both maximum and total amount of space charge increased for the insulation paper contaminated by semi-conductor chemical substance copper sulfide. The space charge decay rate of contaminated paper was significantly enhanced after the polarization voltage was removed. The TSC results revealed that copper sulfide increased the trap density and lowered the shallow trap energy levels. These results contributed to charge transportation by de-trapping and trapping processes. This improved charge transportation could be the main reason for the decreased breakdown voltage of paper insulation material.
Global Energy Development and Climate-Induced Water Scarcity—Physical Limits, Sectoral Constraints, and Policy Imperatives
Authors: Scott Christopher , Sugg Zachary
Published: August 2015

The current accelerated growth in demand for energy globally is confronted by water-resource limitations and hydrologic variability linked to climate change. The global spatial and temporal trends in water requirements for energy development and policy alternatives to address these constraints are poorly understood. This article analyzes national-level energy demand trends from U.S. Energy Information Administration data in relation to newly available assessments of water consumption and life-cycle impacts of thermoelectric generation and biofuel production, and freshwater availability and sectoral allocations from the U.N. Food and Agriculture Organization and the World Bank. Emerging, energy-related water scarcity flashpoints include the world’s largest, most diversified economies (Brazil, India, China, and USA among others), while physical water scarcity continues to pose limits to energy development in the Middle East and small-island states. Findings include the following: (a) technological obstacles to alleviate water scarcity driven by energy demand are surmountable; (b) resource conservation is inevitable, driven by financial limitations and efficiency gains; and (c) institutional arrangements play a pivotal role in the virtuous water-energy-climate cycle. We conclude by making reference to coupled energy-water policy alternatives including water-conserving energy portfolios, intersectoral water transfers, virtual water for energy, hydropower tradeoffs, and use of impaired waters for energy development.
Economic Impact Assessment of Wind Power Integration: A Quasi-Public Goods Property Perspective
Authors: Zhao Huiru , Guo Sen , Li Hongze
Published: August 2015

The integration of wind power into power grid will bring some impacts on the multiple subjects of electric power system. Economic impacts of wind power integration on multiple subjects of China’s electric power system were quantitatively assessed from Quasi-public goods property perspective in this paper. Firstly, the Quasi-public goods property of transmission services provided by power grid corporations was elaborated. Secondly, the multiple subjects of China’s electric power system, which include electricity generation enterprises (EGEs), power grid corporations (PGCs), electricity consumers (ECs), and environment, were detailed analyzed. Thirdly, based on the OPF-based nodal price model and transmission service cost allocation model, the economic impact assessment model of wind power integration was built from Quasi-public goods property perspective. Then, the IEEE-24 bus system employed in this paper was introduced according to current status of China’s electric power system, and the modeling of wind turbine was also introduced. Finally, the simulation analysis was performed, and the economic impacts of wind power integration on EGEs, PGCs, ECs and Environment were calculated. The results indicate, from Quasi-public goods property perspective, the wind power integration will bring positive impacts on EGEs, PGCs and Environment, while negative impacts on ECs. The findings can provide references for power system managers, energy planners, and policy makers.
Unfazed by Both the Bull and Bear: Strategic Exploration in Dynamic Environments
Authors: Riefer Peter , Love Bradley
Published: August 2015

People in a changing environment must decide between exploiting options they currently favor and exploring alternative options that provide additional information about the state of the environment. For example, drivers must decide between purchasing gas at their currently favored station (i.e., exploit) or risk a fruitless trip to another station to evaluate whether the price has been lowered since the last visit. Previous laboratory studies on exploratory choice have found that people choose strategically and explore alternative options when it is more likely that the relative value of competing options has changed. Our study extends this work by considering how global trends (which affect all options equally) influence exploratory choice. For example, during an economic crisis, global gas prices may increase or decrease at all stations, yet consumers should still explore strategically to find the best option. Our research question is whether people can maintain effective exploration strategies in the presence of global trends that are irrelevant in that they do not affect the relative value of choice options. We find that people explore effectively irrespective of global trends.
Can Public Health Risk Assessment Using Risk Matrices Be Misleading?
Authors: Vatanpour Shabnam , Hrudey Steve , Dinu Irina
Published: August 2015

The risk assessment matrix is a widely accepted, semi-quantitative tool for assessing risks, and setting priorities in risk management. Although the method can be useful to promote discussion to distinguish high risks from low risks, a published critique described a problem when the frequency and severity of risks are negatively correlated. A theoretical analysis showed that risk predictions could be misleading. We evaluated a practical public health example because it provided experiential risk data that allowed us to assess the practical implications of the published concern that risk matrices would make predictions that are worse than random. We explored this predicted problem by constructing a risk assessment matrix using a public health risk scenario—Tainted blood transfusion infection risk—That provides negative correlation between harm frequency and severity. We estimated the risk from the experiential data and compared these estimates with those provided by the risk assessment matrix. Although we validated the theoretical concern, for these authentic experiential data, the practical scope of the problem was limited. The risk matrix has been widely used in risk assessment. This method should not be abandoned wholesale, but users must address the source of the problem, apply the risk matrix with a full understanding of this problem and use matrix predictions to inform, but not drive decision-making.
Study on the Adsorption Capacities for Airborne Particulates of Landscape Plants in Different Polluted Regions in Beijing (China)
Authors: Zhang Wei-Kang , Wang Bing , Niu Xiang
Published: August 2015

Urban landscape plants are an important component of the urban ecosystem, playing a significant role in the adsorption of airborne particulates and air purification. In this study, six common landscape plants in Beijing were chosen as research subjects, and the adsorption capacities for each different plant leaf and the effects of the leaf structures for the adsorption capacities for particulates were determined. Preliminary results show that needle-leaved tree species adsorbed more airborne particulates than broad-leaved tree species for the same leaf area. Pinus tabuliformis exhibits the highest adsorption capacity, at 3.89 ± 0.026 μg·cm−2, almost two times as much as that of Populus tomentosa (2.00 ± 0.118 μg·cm−2). The adsorption capacities for PM10 of the same tree species leaves, in different polluted regions had significant differences, and the adsorption capacities for PM10 of the tree species leaf beside the Fifth Ring Road were higher than those of the tree species leaves in the Botanical Garden, although the adsorption capacities for PM2.5 of the same tree species in different polluted regions had no significant differences. By determining the soluble ion concentrations of the airborne particulates in two regions, it is suggested that the soluble ion concentrations of PM10 in the atmosphere in the Botanical Garden and beside the Fifth Ring Road have significant differences, while those of PM2.5 in the atmosphere had no significant differences. In different polluted regions there are significant adaptive changes to the leaf structures, and when compared with slightly polluted region, in the seriously polluted region the epidermis cells of the plant leaves shrinked, the surface textures of the leaves became rougher, and the stomas’ frequency and the pubescence length increased. Even though the plant leaves exposed to the seriously polluted region changed significantly, these plants can still grow normally and healthily.
Prevalence of Polyherbacy in Ambulatory Visits to Traditional Chinese Medicine Clinics in Taiwan
Authors: Lin Ming-Hwai , Chang Hsiao-Ting , Tu Chun-Yi , Chen Tzeng-Ji , Hwang Shinn-Jang
Published: August 2015

Patients with a polyherbal prescription are more likely to receive duplicate medications and thus suffer from adverse drug reactions. We conducted a population-based retrospective study to examine the items of Chinese herbal medicine (CHM) per prescription in the ambulatory care of traditional Chinese medicine (TCM) in Taiwan. We retrieved complete TCM ambulatory visit datasets for 2010 from the National Health Insurance database in Taiwan. A total of 59,790 patients who received 313,482 CHM prescriptions were analyzed. Drug prescriptions containing more than five drugs were classified as polyherbal prescriptions; 41.6% of patients were given a polyherbal prescription. There were on average 5.2 ± 2.5 CHMs: 2.3 ± 1.1 compound herbal formula items, and 3.0 ± 2.5 single Chinese herb items in a single prescription. Approximately 4.6% of patients were prescribed 10 CHMs or more. Men had a lower odds ratio (OR) among polyherbal prescriptions (OR = 0.96, 95% confidence interval [CI] 0.92–0.99), and middle-aged patients (35–49 years) had the highest frequency of polyherbal prescription (OR = 1.19, 95% CI = 1.13–1.26). Patients with neoplasm, skin and subcutaneous tissue disease, or genitourinary system disease were more likely to have a polyherbal prescription; OR = 2.20 (1.81–2.67), 1.65 (1.50–1.80), and 1.52 (1.40–1.64), respectively. Polyherbal prescription is widespread in TCM in Taiwan. Potential herb interactions and iatrogenic risks associated with polyherbal prescriptions should be monitored.
Prevalence of Antimicrobial Resistant and Virulent Salmonella spp. in Treated Effluent and Receiving Aquatic Milieu of Wastewater Treatment Plants in Durban, South Africa
Authors: Odjadjare Ejovwokoghene , Olaniran Ademola
Published: August 2015

In this study, we evaluated the impact of treated wastewater effluent from two wastewater treatment plants on the physicochemical parameters and Salmonella spp. load of receiving rivers. Presumptive Salmonella spp. were obtained at all sampled points including the discharge points, with counts ranging from 0 to 4.14 log cfu/mL at both plants. Turbidity, chemical and biological oxygen demand were found to be high and mostly above the required limit for treated wastewater discharge. However, recorded nitrate and phosphate values were very low. Of the 200 confirmed Salmonella spp. isolates recovered from the treated effluent and receiving surface waters, 93% harbored the spiC gene, 84% harbored the misL gene, and 87.5% harbored the orfL gene while 87% harbored the pipD gene. The antibiotic resistance profile revealed that the isolates were resistant to sulfamethoxazole, nalidixic acid and streptomycin, but susceptible to quinolones and third generation β-lactams. These results indicate that in South Africa treated effluents are still a major source of contamination of rivers with pathogens such as Salmonella. Appropriate steps by the regulatory authorities and workers at the treatment plants are needed to enforce stipulated guidelines in order to prevent pollution of surface water resources due to the discharge of poorly treated effluents.
Risk Factors for Salmonella, Shiga Toxin-Producing Escherichia coli and Campylobacter Occurrence in Primary Production of Leafy Greens and Strawberries
Authors: Ceuppens Siele , Johannessen Gro , Allende Ana , Tondo Eduardo , El-Tahan Fouad , Sampers Imca , Jacxsens Liesbeth , Uyttendaele Mieke
Published: August 2015

The microbiological sanitary quality and safety of leafy greens and strawberries were assessed in the primary production in Belgium, Brazil, Egypt, Norway and Spain by enumeration of Escherichia coli and detection of Salmonella, Shiga toxin-producing E. coli (STEC) and Campylobacter. Water samples were more prone to containing pathogens (54 positives out of 950 analyses) than soil (16/1186) and produce on the field (18/977 for leafy greens and 5/402 for strawberries). The prevalence of pathogens also varied markedly according to the sampling region. Flooding of fields increased the risk considerably, with odds ratio (OR) 10.9 for Salmonella and 7.0 for STEC. A significant association between elevated numbers of generic E. coli and detection of pathogens (OR of 2.3 for STEC and 2.7 for Salmonella) was established. Generic E. coli was found to be a suitable index organism for Salmonella and STEC, but to a lesser extent for Campylobacter. Guidelines on frequency of sampling and threshold values for E. coli in irrigation water may differ from region to region.
Genes and Pathways Involved in Adult Onset Disorders Featuring Muscle Mitochondrial DNA Instability
Authors: Ahmed Naghia , Ronchi Dario , Comi Giacomo
Published: August 2015

Replication and maintenance of mtDNA entirely relies on a set of proteins encoded by the nuclear genome, which include members of the core replicative machinery, proteins involved in the homeostasis of mitochondrial dNTPs pools or deputed to the control of mitochondrial dynamics and morphology. Mutations in their coding genes have been observed in familial and sporadic forms of pediatric and adult-onset clinical phenotypes featuring mtDNA instability. The list of defects involved in these disorders has recently expanded, including mutations in the exo-/endo-nuclease flap-processing proteins MGME1 and DNA2, supporting the notion that an enzymatic DNA repair system actively takes place in mitochondria. The results obtained in the last few years acknowledge the contribution of next-generation sequencing methods in the identification of new disease loci in small groups of patients and even single probands. Although heterogeneous, these genes can be conveniently classified according to the pathway to which they belong. The definition of the molecular and biochemical features of these pathways might be helpful for fundamental knowledge of these disorders, to accelerate genetic diagnosis of patients and the development of rational therapies. In this review, we discuss the molecular findings disclosed in adult patients with muscle pathology hallmarked by mtDNA instability.
Exploiting the Pleiotropic Antioxidant Effects of Established Drugs in Cardiovascular Disease
Authors: Steven Sebastian , Münzel Thomas , Daiber Andreas
Published: August 2015

Cardiovascular disease is a leading cause of death and reduced quality of life worldwide. Arterial vessels are a primary target for endothelial dysfunction and atherosclerosis, which is accompanied or even driven by increased oxidative stress. Recent research in this field identified different sources of reactive oxygen and nitrogen species contributing to the pathogenesis of endothelial dysfunction. According to lessons from the past, improvement of endothelial function and prevention of cardiovascular disease by systemic, unspecific, oral antioxidant therapy are obviously too simplistic an approach. Source- and cell organelle-specific antioxidants as well as activators of intrinsic antioxidant defense systems might be more promising. Since basic research demonstrated the contribution of different inflammatory cells to vascular oxidative stress and clinical trials identified chronic inflammatory disorders as risk factors for cardiovascular events, atherosclerosis and cardiovascular disease are closely associated with inflammation. Therefore, modulation of the inflammatory response is a new and promising approach in the therapy of cardiovascular disease. Classical anti-inflammatory therapeutic compounds, but also established drugs with pleiotropic immunomodulatory abilities, demonstrated protective effects in various models of cardiovascular disease. However, results from ongoing clinical trials are needed to further evaluate the value of immunomodulation for the treatment of cardiovascular disease.
Current Status on Stem Cells and Cancers of the Gastric Epithelium
Authors: Hoffmann Werner
Published: August 2015

Gastric cancer is still a leading cause of cancer-related mortality worldwide in spite of declining incidence. Gastric cancers are, essentially, adenocarcinomas and one of the strongest risk factors is still infection with Helicobacter pylori. Within the last years, it became clear that gastric self-renewal and carcinogenesis are intimately linked, particularly during chronic inflammatory conditions. Generally, gastric cancer is now regarded as a disease resulting from dysregulated differentiation of stem and progenitor cells, mainly due to an inflammatory environment. However, the situation in the stomach is rather complex, consisting of two types of gastric units which show bidirectional self-renewal from an unexpectedly large variety of progenitor/stem cell populations. As in many other tumors, cancer stem cells have also been characterized for gastric cancer. This review focuses on the various gastric epithelial stem cells, how they contribute to self-renewal and which routes are known to gastric adenocarcinomas, including their stem cells.
Cdc42-Interacting Protein 4 Represses E-Cadherin Expression by Promoting β-Catenin Translocation to the Nucleus in Murine Renal Tubular Epithelial Cells
Authors: Xu Chuou , Zhou Qiaodan , Liu Lili , Liu Ping , Pei Guangchang , Zeng Rui , Han Min , Xu Gang
Published: August 2015

Renal fibrosis is an inevitable outcome of end-stage chronic kidney disease. During this process, epithelial cells lose E-cadherin expression. β-Catenin may act as a mediator by accumulation and translocation to the nucleus. Studies have suggested that CIP4, a Cdc42 effector protein, is associated with β-catenin. However, whether CIP4 contributes to E-cadherin loss in epithelial cells by regulating β-catenin translocation is unclear. In this study, we investigated the involvement of CIP4 in β-catenin translocation. Expression of CIP4 was upregulated in renal tissues of 5/6 nephrectomized rats and mainly distributed in renal tubular epithelia. In TGF-β1-treated NRK-52E cells, upregulation of CIP4 expression was accompanied by reduced expression of E-cadherin. CIP4 overexpression promoted the translocation of β-catenin to the nucleus, which was accompanied by reduced expression of E-cadherin even without TGF-β1 stimulation. In contrast, CIP4 depletion by using siRNA inhibited the translocation of β-catenin to the nucleus and reversed the decrease in expression of E-cadherin. The interaction between CIP4 and β-catenin was detected. We also show that β-catenin depletion could restore the expression of E-cadherin that was suppressed by CIP4 overexpression. In conclusion, these results suggest that CIP4 overexpression represses E-cadherin expression by promoting β-catenin translocation to the nucleus.
Lentiviral-Mediated Short Hairpin RNA Knockdown of MTDHInhibits Cell Growth and Induces Apoptosis by Regulatingthe PTEN/AKT Pathway in Hepatocellular Carcinoma
Authors: Li Wen-Fang , Ou Qin , Dai Hang , Liu Chang-An
Published: August 2015

The activation of oncogenes and the loss of tumor suppressor genes are believed toplay critical roles in the pathogenesis of human hepatocellular carcinoma (HCC). Metaherin (MTDH), also called astrocyte elevated gene-1 (AEG-1), is frequently amplified in a variety of cancers, but the roles of MTDH with regard to growth and apoptosis in HCC have not yet been studied. In the present study, we first analyzed the expression of MTDH in HCC samples. We found that MTDH protein levels are higher in most HCC cancerous tissues compared with their matched adjacent non-tumor tissues. Additionally, the MTDH mRNA was also higher in HCC tissues compared to their matched adjacent non-tumor tissues. Knockdown of the endogenous MTDH using small interfering RNA further showed that deficiency of MTDH suppressed cell growth and caused apoptosis in HCC cells. Knockdown MTDH promoted PTEN and p53 expression in HCC cells and inhibited AKT phosphorylation. Knockdown MTDH also inhibited tumor growth in vivo. All these results indicated that MTDH protein levels in most HCC tissues are higher than non-tumor tissues, and knockdown of MTDH inhibited growth and induced apoptosis in HCC cells through the activation of PTEN. Therefore, MTDH might be an effective targeted therapy gene for HCC.
Terpenoids from the Octocoral Sinularia gaweli
Authors: Lin Wun-Jie , Wu Tung-Ying , Su Tzu-Rong , Wen Zhi-Hong , Chen Jih-Jung , Fang Lee-Shing , Wu Yang-Chang , Sung Ping-Jyun
Published: August 2015

Two eudesmane sesquiterpenoids, verticillatol (1) and 5α-acetoxy-4(14)-eudesmene-1β-ol (2) and two cembrane diterpenoids, (–)-leptodiol acetate (3) and sinulacembranolide A (4) were isolated from the octocoral Sinularia gaweli and compounds 2–4 are new isolates. The structures of new terpenoids 2–4 were elucidated by spectroscopic methods and by comparison the spectral data with those of known analogues. Terpenoid 4 was found to inhibit the accumulation of the pro-inflammatory inducible nitric oxide synthase (iNOS) protein of the lipopolysaccharide (LPS)-stimulated RAW264.7 marcophage cells.
Black Box Traceable Ciphertext Policy Attribute-Based Encryption Scheme
Authors: Fu Xingbing , Nie Xuyun , Li Fagen
Published: August 2015

In the existing attribute-based encryption (ABE) scheme, the authority (i.e., private key generator (PKG)) is able to calculate and issue any user’s private key, which makes it completely trusted, which severely influences the applications of the ABE scheme. To mitigate this problem, we propose the black box traceable ciphertext policy attribute-based encryption (T-CP-ABE) scheme in which if the PKG re-distributes the users’ private keys for malicious uses, it might be caught and sued. We provide a construction to realize the T-CP-ABE scheme in a black box model. Our scheme is based on the decisional bilinear Diffie-Hellman (DBDH) assumption in the standard model. In our scheme, we employ a pair (ID, S) to identify a user, where ID denotes the identity of a user and S denotes the attribute set associated with her.
Dynamic Heterogeneity of the Heart Valve Interstitial Cell Population in Mitral Valve Health and Disease
Authors: Horne Tori , VandeKopple Matthew , Sauls Kimberly , Koenig Sara , Anstine Lindsey , Garg Vidu , Norris Russell , Lincoln Joy
Published: August 2015

The heart valve interstitial cell (VIC) population is dynamic and thought to mediate lay down and maintenance of the tri-laminar extracellular matrix (ECM) structure within the developing and mature valve throughout life. Disturbances in the contribution and distribution of valve ECM components are detrimental to biomechanical function and associated with disease. This pathological process is associated with activation of resident VICs that in the absence of disease reside as quiescent cells. While these paradigms have been long standing, characterization of this abundant and ever-changing valve cell population is incomplete. Here we examine the expression pattern of Smooth muscle α-actin, Periostin, Twist1 and Vimentin in cultured VICs, heart valves from healthy embryonic, postnatal and adult mice, as well as mature valves from human patients and established mouse models of disease. We show that the VIC population is highly heterogeneous and phenotypes are dependent on age, species, location, and disease state. Furthermore, we identify phenotypic diversity across common models of mitral valve disease. These studies significantly contribute to characterizing the VIC population in health and disease and provide insights into the cellular dynamics that maintain valve structure in healthy adults and mediate pathologic remodeling in disease states.
MicroRNAs: Emerging Novel Clinical Biomarkers for Hepatocellular Carcinomas
Authors: Anwar Sumadi , Lehmann Ulrich
Published: August 2015

The discovery of small non-coding RNAs known as microRNAs has refined our view of the complexity of gene expression regulation. In hepatocellular carcinoma (HCC), the fifth most frequent cancer and the third leading cause of cancer death worldwide, dysregulation of microRNAs has been implicated in all aspects of hepatocarcinogenesis. In addition, alterations of microRNA expression have also been reported in non-cancerous liver diseases including chronic hepatitis and liver cirrhosis. MicroRNAs have been proposed as clinically useful diagnostic biomarkers to differentiate HCC from different liver pathologies and healthy controls. Unique patterns of microRNA expression have also been implicated as biomarkers for prognosis as well as to predict and monitor therapeutic responses in HCC. Since dysregulation has been detected in various specimens including primary liver cancer tissues, serum, plasma, and urine, microRNAs represent novel non-invasive markers for HCC screening and predicting therapeutic responses. However, despite a significant number of studies, a consensus on which microRNA panels, sample types, and methodologies for microRNA expression analysis have to be used has not yet been established. This review focuses on potential values, benefits, and limitations of microRNAs as new clinical markers for diagnosis, prognosis, prediction, and therapeutic monitoring in HCC.
Effect of Stress Amplitude on the Damping of Recycled Aggregate Concrete
Authors: Liang Chaofeng , Liu Tiejun , Xiao Jianzhuang , Zou Dujian , Yang Qiuwei
Published: August 2015

Damping characterizes the energy dissipation capacity of materials and structures, and it is affected by several external factors such as vibrating frequency, stress history, temperature, and stress amplitude. This study investigates the relationship between the damping and the stress amplitude of environment-friendly recycled aggregate concrete (RAC). First, a function model of a member’s loss factor and stress amplitude was derived based on Lazan’s damping-stress function. Then, the influence of stress amplitude on the loss tangent of RAC was experimentally investigated. Finally, parameters used to determine the newly derived function were obtained by numerical fitting. It is shown that the member’s loss factor is affected not only by the stress amplitude but also by factors such as the cross section shapes, boundary conditions, load types, and loading positions. The loss tangent of RAC increases with the stress amplitude, even at low stress amplitude. The damping energy exponent of RAC is not identically equal to 2.0, indicating that the damping is nonlinear. It is also found that the energy dissipation capacity of RAC is superior to that of natural aggregate concrete (NAC), and the energy dissipation capacity can be further improved by adding modified admixtures.
Effect of Annealing Temperature and Oxygen Flow in the Properties of Ion Beam Sputtered SnO—2x Thin Films
Authors: Wang Chun-Min , Huang Chun-Chieh , Kuo Jui-Chao , Sahu Dipti , Huang Jow-Lay
Published: August 2015

Tin oxide (SnO2—x) thin films were prepared under various flow ratios of O2/(O2 + Ar) on unheated glass substrate using the ion beam sputtering (IBS) deposition technique. This work studied the effects of the flow ratio of O2/(O2 + Ar), chamber pressures and post-annealing treatment on the physical properties of SnO2 thin films. It was found that annealing affects the crystal quality of the films as seen from both X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis. In addition, the surface RMS roughness was measured with atomic force microscopy (AFM). Auger electron spectroscopy (AES) analysis was used to obtain the changes of elemental distribution between tin and oxygen atomic concentration. The electrical property is discussed with attention to the structure factor.
A Moonshine Dialogue in Mathematical Physics
Authors: Planat Michel
Published: August 2015

Phys and Math are two colleagues at the University of Saçenbon (Crefan Kingdom), dialoguing about the remarkable efficiency of mathematics for physics. They talk about the notches on the Ishango bone and the various uses of psi in maths and physics; they arrive at dessins d’enfants, moonshine concepts, Rademacher sums and their significance in the quantum world. You should not miss their eccentric proposal of relating Bell’s theorem to the Baby Monster group. Their hyperbolic polygons show a considerable singularity/cusp structure that our modern age of computers is able to capture. Henri Poincaré would have been happy to see it.
Hormaomycins B and C: New Antibiotic Cyclic Depsipeptides from a Marine Mudflat-Derived Streptomyces sp.
Authors: Bae Munhyung , Chung Beomkoo , Oh Ki-Bong , Shin Jongheon , Oh Dong-Chan
Published: August 2015

Alterations in microbial culture conditions may trigger the production of diverse bioactive secondary metabolites. While applying various culture conditions and monitoring secondary metabolite profiles using LC/MS, hormaomycins B and C (1 and 2) were discovered from a marine mudflat-derived actinomycete, Streptomyces sp., collected in Mohang, Korea. The planar structures of the hormaomycins, which bear structurally-unique units, such as 4-(Z)-propenylproline, 3-(2-nitrocyclopropyl)alanine, 5-chloro-1-hydroxypyrrol-2-carboxylic acid and b-methylphenylalanine, were established as the first natural analogues belonging to the hormaomycin peptide class. The absolute configurations of 1 and 2 were deduced by comparing their CD spectra with that of hormaomycin. These hormaomycins exhibited significant inhibitory effects against various pathogenic Gram-positive and Gram-negative bacteria.