bluuebunny/crossref_metadata_2025 huggingface dataset:
Explore the effect of pressure and time of compression on the risk of intraoperatively acquired pressure injury based on theoretical framework: A prospective study
Authors: Chen Yuan , Wang Wei , Qian QianJian , Zha Qinghua , Wu BeiWen
Published: April 2024

Surgery is a high risk factor for the occurrence of pressure injury (PI). On the basis of theoretical research, pressure and duration of pressure are key factors affecting PI. Pressure is affected by the individual pressure redistribution capacity. So our study aims to explore how the surgery time and pressure intensity affect the occurrence of PI and what are the risk factors. A prospective study. A total of 250 patients who underwent elective surgery in a grade‐A general hospital from November 2021 to February 2023 were selected and divided into a group of 77 patients with IAPI (intraoperatively acquired pressure injury) and a group of 173 patients with no IAPI. Visual pressure inductive feedback system and body composition analysis technology were used to record the local pressure value and change of patients before and after anaesthesia. Relevant data of the patients were collected to explore the influencing factors. The maximum pressure and average pressure at the pressure site of the same patient changed before and after anaesthesia, and the pressure after anaesthesia was significantly higher than that before anaesthesia. There was no statistical difference in the average pressure after anaesthesia (p > 0.05), but the maximum pressure in the IAPI group was higher than that in the non‐occurrence group (p < 0.05). The average pressure multiplied by the operation time in IAPI group is significantly higher than that in the non‐IAPI group (p < 0.01). Multiple linear regression analysis (stepwise regression) showed that fat‐free weight, age, waist circumference, body mass index (BMI) and gender were taken as independent variables into the regression model, affecting the maximum pressure. In addition, operation time ≥4 h may be a high risk factor for IAPI. In future studies, more objective research tools can be applied to improve the accuracy of predicting the risk of IAPI. In addition to gender and BMI, follow‐up studies may consider including measures such as waist circumference and fat‐free body weight in IAPI risk assessment to guide the clinical nursing work more scientifically.
Thermochemical interactions between yttria‐stabilized zirconia and molten lunar regolith simulants
Authors: Yu Kevin , Stokes Jamesa , Harder Bryan , Reidy Lorlyn , Faber Katherine T.
Published: April 2024

Oxygen produced through in‐situ resource utilization (ISRU) is critical to maintaining a permanent human presence on the lunar surface. Molten regolith electrolysis and carbothermal reduction are two promising ISRU techniques for generating oxygen directly from lunar regolith, which is primarily a mixture of oxide minerals; however, both processes require operating temperatures of 1600°C to melt lunar regolith and dissociate the molten oxides. These conditions limit the use of many oxide refractory materials, such as Al2O3 and MgO, due to rapid degradation resulting from reactions between the refractory materials and molten lunar regolith. Yttria‐stabilized zirconia (YSZ) is shown here to be a promising refractory oxide to provide containment of molten regolith while demonstrating limited reactivity. This work focuses on corrosion studies of YSZ powders and dense YSZ crucibles in contact with molten lunar maria and highlands regolith simulants at 1600°C. The interactions between YSZ and molten regolith were characterized using scanning electron microscopy/energy dispersive spectroscopy, X‐ray diffraction, and electron backscatter diffraction. A FactSage thermochemical model was created for comparison with the experimental results. These combined analyses suggest that lunar maria regolith will degrade the YSZ faster than the lunar highlands regolith due to the lower viscosity of the maria regolith. The feasibility of long‐term molten regolith containment with YSZ is discussed based on the YSZ powder and crucible results.
Circular genetic structure of the Abies nephrolepis species complex shaped by the circular landform of Northeast Asia
Authors: Kwak Myounghai , Nakamura Koh , Xiang Qiaoping , Wen Jun , Lee Eun Sil , Hong Jaram , Kovtonyuk Nataliya , Kryukova Maria , Korchagina Olga , Koo Kyung Ah , Iwaizumi Masakazu G. , Lee Byoung Yoon , Kokubugata Goro , Zhang Xianchun , Lebedev Dmitry
Published: April 2024

Aim

Pinaceae have been noted for their tendency towards reticulation as a result of interspecific hybridization. Here, we demonstrated the phylogeographic dynamics of the native Northeast Asian sub‐alpine conifer, Abies nephrolepis species complex (ANSC), evolving in circular overlaps along the Northeast Asian landform, which functions as a corridor with the sea, thus acting as a geographic barrier.

Location

Northeast Asia: the Korean Peninsula, Japanese Archipelago, Russian Far East and northeastern China.

Taxon

Abies nephrolepis species complex (Family Pinaceae, Genus Abies, Section Balsamea, Abies nephrolepis, A. koreana, A. veitchii and A. sachalinensis).

Methods

A total of 728 individuals from 38 ANSC populations were analysed using multiplexed inter‐simple sequence repeat genotyping by sequencing to capture variations in biparental nuclear genomes. Eight mitochondrial regions and eight chloroplast regions of each individual were sequenced using the MiSeq platform and Sanger sequencing. Species distribution models were generated.

Results

Bayesian clustering with 507 nuclear single nucleotide polymorphisms and the discrepancy between cytoplasmic and nuclear genome lineages implied contemporary and ancient connections between neighbouring species in the form of circular overlap. This genetic connectivity was supported by principal component analysis. Strong correlations between genetic distance and geographic distance were observed, suggesting that gene flow occurs through a continuous chain around the sea. We also found that gene flow direction and intensity changed over time, with support from palaeodistribution modelling.

Conclusion

Past hybridization events were captured in cytoplasmic genomes, generating heterogeneity across maternal ancestries. This intensive phylogeographic study demonstrates speciation with incomplete reproductive isolation (continuous gene flow) among neighbouring species with an alteration of the direction and intensity of gene flow due to climate change. The divergence of ANSC due to repeated isolation and reconnection caused by heterogenous physiological environments and climate fluctuation provides a model to solve evolutionary scenarios for reticulate evolution in Pinaceae and other plants.
The plasma miRNome and venous thromboembolism in high‐grade glioma: miRNA Sequencing of a nested case–control cohort
Authors: Erhart Friedrich , Widhalm Georg , Kiesel Barbara , Hackl Matthias , Diendorfer Andreas , Preusser Matthias , Rössler Karl , Thaler Johannes , Pabinger Ingrid , Ay Cihan , Riedl Julia
Published: April 2024

Patients with high‐grade gliomas are at high risk of venous thromboembolism (VTE). MicroRNAs (miRNAs) are small non‐coding RNAs with multiple roles in tumour biology, haemostasis and platelet function. Their association with VTE risk in high‐grade glioma has not been comprehensively mapped so far. We thus conducted a nested case–control study within 152 patients with WHO grade IV glioma that had been part of a prospective cohort study on VTE risk factors. At inclusion a single blood draw was taken, and patients were thereafter followed for a maximum of 2 years. During that time, 24 patients (16%) developed VTE. Of the other 128 patients, we randomly selected 24 age‐ and sex‐matched controls. After quality control, the final group size was 21 patients with VTE during follow‐up and 23 without VTE. Small RNA next‐generation sequencing of plasma was performed. We observed that hsa‐miR‐451a was globally the most abundant miRNA. Notably, 51% of all miRNAs showed a correlation with platelet count. The analysis of miRNAs differentially regulated in VTE patients—with and without platelet adjustment—identified potential VTE biomarker candidates such as has‐miR‐221‐3p. Therewith, we here provide one of the largest and deepest peripheral blood miRNA datasets of high‐grade glioma patients so far, in which we identified first VTE biomarker candidates that can serve as the starting point for future research.
Pathway‐based stratification of gliomas uncovers four subtypes with different TME characteristics and prognosis
Authors: Huang Ruoyu , Han Bo , Zhang Ying , Yang Jingchen , Wang Kuanyu , Liu Xing , Wang Zhiliang
Published: April 2024

Increasing evidences have found that the interactions between hypoxia, immune response and metabolism status in tumour microenvironment (TME) have clinical importance of predicting clinical outcomes and therapeutic efficacy. This study aimed to develop a reliable molecular stratification based on these key components of TME. The TCGA data set (training cohort) and two independent cohorts from CGGA database (validation cohort) were enrolled in this study. First, the enrichment score of 277 TME‐related signalling pathways was calculated by gene set variation analysis (GSVA). Then, consensus clustering identified four stable and reproducible subtypes (AFM, CSS, HIS and GLU) based on TME‐related signalling pathways, which were characterized by differences in hypoxia and immune responses, metabolism status, somatic alterations and clinical outcomes. Among the four subtypes, HIS subtype had features of immunosuppression, oxygen deprivation and active energy metabolism, resulting in a worst prognosis. Thus, for better clinical application of this acquired stratification, we constructed a risk signature by using the LASSO regression model to identify patients in HIS subtype accurately. We found that the risk signature could accurately screen out the patients in HIS subtype and had important reference value for individualized treatment of glioma patients. In brief, the definition of the TME‐related subtypes was a valuable tool for risk stratification in gliomas. It might serve as a reliable prognostic classifier and provide rational design of individualized treatment, and follow‐up scheduling for patients with gliomas.
Integrated analysis highlights the significance role of ITGAL in lung adenocarcinoma
Authors: Xiao Zengtuan , Nian Zhe , Zhang Mengzhe , Liu Zuo , Liu Zhe , Zhang Zhenfa
Published: April 2024

Integrin alpha L (ITGAL), a member of the integrin family, is associated with carcinogenesis and immune regulation. However, the biological functions of ITGAL in lung adenocarcinoma (LUAD) remain poorly understood. In this study, we utilized the TCGA dataset to analyse ITGAL mRNA expression in LUAD and examined its correlation with clinical prognosis. Three‐dimensional (3D) Matrigel culture, 5‐bromodeoxyuridine (BrdU) ELISA, wound‐healing migration and cell adherence assays were used to demonstrate the potential role of ITGAL in LUAD progression. Additionally, we analysed single‐cell sequencing data of LUAD to determine the expression and biological function of ITGAL. Our research revealed that the expression of ITGAL in LUAD samples is an independent predictor of prognosis. Patients with high expression of ITGAL had significantly better overall survival (OS), progression‐free survival (PFS) and disease‐specific survival (DSS) compared to the low‐expression group. Meanwhile, the expression of ITGAL suppressed malignant progression in LUAD cells. Functional enrichment analyses showed that ITGAL was significantly correlated with cell immune response and immune checkpoint, consistent with the analysis of single‐cell sequencing in paired samples of normal and tumour. Furthermore, we confirmed that ITGAL expression affect the tumour microenvironment (TME) through regulation of the expression of cytokines in NK cells of LUAD. In summary, ITGAL is a prognostic biomarker for LUAD patients, and it repressed malignant progression in LUAD cells. Moreover, ITGAL expression also enhanced the effect of immunotherapy and may be an important target in LUAD therapy.
The role of autophagy in odontogenesis, dental implant surgery, periapical and periodontal diseases
Authors: İnan Sevinç , Barış Emre
Published: April 2024

Autophagy is a cellular process that is evolutionarily conserved, involving the sequestration of damaged organelles and proteins into autophagic vesicles, which subsequently fuse with lysosomes for degradation. Autophagy controls the development of many diseases by influencing apoptosis, inflammation, the immune response and different cellular processes. Autophagy plays a significant role in the aetiology of disorders associated with dentistry. Autophagy controls odontogenesis. Furthermore, it is implicated in the pathophysiology of pulpitis and periapical disorders. It enhances the survival, penetration and colonization of periodontal pathogenic bacteria into the host periodontal tissues and facilitates their escape from host defences. Autophagy plays a crucial role in mitigating exaggerated inflammatory reactions within the host's system during instances of infection and inflammation. Autophagy also plays a role in the relationship between periodontal disease and systemic diseases. Autophagy promotes wound healing and may enhance implant osseointegration. This study reviews autophagy's dento‐alveolar effects, focusing on its role in odontogenesis, periapical diseases, periodontal diseases and dental implant surgery, providing valuable insights for dentists on tooth development and dental applications. A thorough examination of autophagy has the potential to discover novel and efficacious treatment targets within the field of dentistry.
Deferoxamine topical cream superior to patch in rescuing radiation‐induced fibrosis of unwounded and wounded skin
Authors: Berry Charlotte E. , Abbas Darren B. , Griffin Michelle , Lintel Hendrik , Guo Jason , Kameni Lionel , Churukian Andrew A. , Fazilat Alexander Z. , Chen Kellen , Gurtner Geoffrey C. , Longaker Michael T. , Momeni Arash , Wan Derrick C.
Published: April 2024

Topical patch delivery of deferoxamine (DFO) has been studied as a treatment for this fibrotic transformation in irradiated tissue. Efficacy of a novel cream formulation of DFO was studied as a RIF therapeutic in unwounded and excisionally wounded irradiated skin. C57BL/6J mice underwent 30 Gy of radiation to the dorsum followed by 4 weeks of recovery. In a first experiment, mice were separated into six conditions: DFO 50 mg cream (D50), DFO 100 mg cream (D100), soluble DFO injections (DI), DFO 1 mg patch (DP), control cream (Vehicle), and irradiated untreated skin (IR). In a second experiment, excisional wounds were created on the irradiated dorsum of mice and then divided into four treatment groups: DFO 100 mg Cream (W‐D100), DFO 1 mg patch (W‐DP), control cream (W‐Vehicle), and irradiated untreated wounds (W‐IR). Laser Doppler perfusion scans, biomechanical testing, and histological analysis were performed. In irradiated skin, D100 improved perfusion compared to D50 or DP. Both D100 and DP enhanced dermal characteristics, including thickness, collagen density and 8‐isoprostane staining compared to untreated irradiated skin. D100 outperformed DP in CD31 staining, indicating higher vascular density. Extracellular matrix features of D100 and DP resembled normal skin more closely than DI or control. In radiated excisional wounds, D100 facilitated faster wound healing and increased perfusion compared to DP. The 100 mg DFO cream formulation rescued RIF of unwounded irradiated skin and improved excisional wound healing in murine skin relative to patch delivery of DFO.
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosisIIIC(Sanfilippo C syndrome)
Authors: Zhao Hongjun , Wang Lijing , Zhang Mengfei , Wang Huakun , Zhang Sizhe , Wu Junjiao , Tang Yu
Published: April 2024

Mucopolysaccharidosis type IIIC (MPS IIIC) is one of inherited lysosomal storage disorders, caused by deficiencies in lysosomal hydrolases degrading acidic mucopolysaccharides. The gene responsible for MPS IIIC isHGSNAT, which encodes an enzyme that catalyses the acetylation of the terminal glucosamine residues of heparan sulfate. So far, few studies have focused on the genetic landscape of MPS IIIC in China, where IIIA and IIIB were the major subtypes. In this study, we utilized whole‐exome sequencing (WES) to identify novel compound heterozygous variants in theHGSNATgene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathogenic variants, which is of importance to dissect the pathogenesis and to carry out clinical diagnosis of MPS IIIC. Moreover, this study helps to depict the natural history of Chinese MPS IIIC populations.
Comprehensive analysis of the interaction of antigen presentation during anti‐tumour immunity and establishment of AIDPS systems in ovarian cancer
Authors: Sun Wenhuizi , Xu Ping , Gao Kefei , Lian Wenqin , Sun Xiang
Published: April 2024

There are hundreds of prognostic models for ovarian cancer. These genes are based on different gene classes, and there are many ways to construct the models. Therefore, this paper aims to build the most stable prognostic evaluation system known to date through 101 machine learning strategies. We combined 101 algorithm combinations with 10 machine learning algorithms to create antigen presentation‐associated genetic markers (AIDPS) with outstanding precision and steady performance. The inclusive set of algorithms comprises the elastic network (Enet), Ridge, stepwise Cox, Lasso, generalized enhanced regression model (GBM), random survival forest (RSF), supervised principal component (SuperPC), Cox partial least squares regression (plsRcox), survival support vector machine (Survival‐SVM). Then, in the train cohort, the prediction model was fitted using a leave‐one cross‐validation (LOOCV) technique, which involved 101 different possible combinations of prognostic genes. Seven validation data sets (GSE26193, GSE26712, GSE30161, GSE63885, GSE9891, GSE140082 and ICGC_OV_AU) were compared and analysed, and the C‐index was calculated. Finally, we collected 32 published ovarian cancer prognostic models (including mRNA and lncRNA). All data sets and prognostic models were subjected to a univariate Cox regression analysis, and the C‐index was calculated to demonstrate that the antigen presentation process should be the core criterion for evaluating ovarian cancer prognosis. In a univariate Cox regression analysis, 22 prognostic genes were identified based on the expression profiles of 283 genes involved in antigen presentation and the intersection of genes (p < 0.05). AIDPS were developed by our machine learning‐based integration method, which was applied to these 22 genes. One hundred and one prediction models are fitted using the LOOCV framework, and the C‐index is calculated for each model across all validation sets. Interestingly, RSF + Lasso was the best model overall since it had the greatest average C‐index and the highest C‐index of any combination of models tested on the validated data sets. In comparing external cohorts, we found that the C‐index correlated AIDPS method using the RSF + Lasso method in 101 prediction models was in contrast to other features. Notably, AIDPS outperformed the vast majority of models across all data sets. Antigen‐presenting anti‐tumour immune pathways can be used as a representative gene set of ovarian cancer to track the prognosis of patients with cancer. The antigen‐presenting model obtained by the RSF + Lasso method has the best C‐INDEX, which plays a key role in developing antigen‐presenting targeted drugs in ovarian cancer and improving the treatment outcome of patients.
The adjunctive effect of polydeoxyribonucleotide on bone formation in alveolar ridge preservation: A pre‐clinical in vivo study
Authors: Ko Young‐Chang , Lee Jungwon , Urban Istvan , Seol Yang‐Jo , Lee Yong‐Moo , Koo Ki‐Tae
Published: April 2024

Aim

This study investigated the adjunctive effect of polydeoxyribonucleotide (PDRN) on bone formation in alveolar ridge preservation (ARP) sockets.

Materials and Methods

Both mandibular second, third and fourth premolars of eight beagle dogs were randomly divided into ARP and ARP/PDRN groups. Following tooth extraction, ARP procedures were conducted using collagenized alloplastic graft material and bilayer collagen membrane soaked with normal saline (ARP group) or PDRN (ARP/PDRN group) for 10 min before application. Both groups were also randomly allocated to 2‐, 4‐ or 12‐week healing subgroups. The primary endpoint of this study was to compare histomorphometric differences between ARP and ARP/PDRN. The secondary endpoints of this study were to compare micro‐CT analysis and three‐dimensional volumetric measurement between the two groups.

Results

In the histomorphometric analysis, the ARP/PDRN group exhibited greater new bone formation at coronal, middle and total position compared with the ARP group at 2‐week healing. The number of newly formed blood vessels was higher in the ARP/PDRN group than in the ARP group at 2‐ and 4‐week healing. In micro‐CT analysis, the mean new bone volume/total bone volume between ARP and ARP/PDRN was statistically significant at 2‐week healing. Ridge volume alterations were significantly decreased in the ARP/PDRN group during entire healing time compared with the ARP group, especially on the buccal side.

Conclusions

The application of PDRN in ARP might provide additional benefits for early bone regeneration and maintenance of buccal ridge volume.
The relationship between type, timing and duration of exposure to adverse childhood experiences and adolescent self‐harm and depression: findings from three UK prospective population‐based cohorts
Authors: Farooq Bushra , Russell Abigail E. , Howe Laura D. , Herbert Annie , Smith Andrew D.A.C. , Fisher Helen L. , Baldwin Jessie R. , Arseneault Louise , Danese Andrea , Mars Becky
Published: April 2024

Background

Adverse childhood experiences (ACEs) are well‐established risk factors for self‐harm and depression. However, despite their high comorbidity, there has been little focus on the impact of developmental timing and the duration of exposure to ACEs on co‐occurring self‐harm and depression.

Methods

Data were utilised from over 22,000 children and adolescents participating in three UK cohorts, followed up longitudinally for 14–18 years: the Avon Longitudinal Study of Parents and Children (ALSPAC), the Millennium Cohort Study (MCS) and the Environmental Risk (E‐Risk) Longitudinal Twin Study. Multinomial logistic regression models estimated associations between each ACE type and a four‐category outcome: no self‐harm or depression, self‐harm alone, depression alone and self‐harm with co‐occurring depression. A structured life course modelling approach was used to examine whether the accumulation (duration) of exposure to each ACE, or a critical period (timing of ACEs) had the strongest effects on self‐harm and depression in adolescence.

Results

The majority of ACEs were associated with co‐occurring self‐harm and depression, with consistent findings across cohorts. The importance of timing and duration of ACEs differed across ACEs and across cohorts. For parental mental health problems, longer duration of exposure was strongly associated with co‐occurring self‐harm and depression in both ALSPAC (adjusted OR: 1.18, 95% CI: 1.10–1.25) and MCS (1.18, 1.11–1.26) cohorts. For other ACEs in ALSPAC, exposure in middle childhood was most strongly associated with co‐occurring self‐harm and depression, and ACE occurrence in early childhood and adolescence was more important in the MCS.

Conclusions

Efforts to mitigate the impact of ACEs should start in early life with continued support throughout childhood, to prevent long‐term exposure to ACEs contributing to risk of self‐harm and depression in adolescence.
Redescription and molecular characterization of Loxocephalus luridus Eberhard, 1862 based on Czech populations: Implications for order Loxocephalida Jankowski, 1980
Authors: Bourland William , Pomahač Ondřej , Poláková Kateřina , Čepička Ivan
Published: April 2024

The phylogenetic and taxonomic affinities of lineages currently assigned to the non‐monophyletic ciliate order Loxocephalida Jankowski (1980) within subclass Scuticociliatia Small (1967) remain unresolved. In the current study, we redescribe the morphology of the type species, Loxocephalus luridus Eberhard (1862) based on two Czech populations and include the first scanning and transmission electron microscopy images of the species. We provide the first 18S rRNA gene sequences for L. luridus and consider its phylogenetic position. Our results support the separation of Dexiotricha from Loxocephalus; however, the former genus is recovered as non‐monophyletic. The monophyly of genus Dexiotricha and that of Loxocephalus + Dexiotricha is rejected. Loxocephalus luridus, together with Dexiotricha species, nests within a fully supported clade with Conchophthirus species, long presumed to belong to the Pleuronematida. Haptophrya is recovered as sister to this clade. The monophyly of the Astomatia Schewiakoff (1896) including Haptophrya is rejected. No clear morphologic synapomorphy is identified for the fully supported clade consisting of Haptophrya, Dexiotricha, Loxocephalus, and Conchophthirus.
Sensitivity analysis on stormwater management response to land cover dynamics and urban expansion of developing City in Lake Hawassa watershed, Ethiopia
Authors: Kassay Abreham Birhane , Tuhar Abraham Woldemichael , Ulsido Mihret Dananto
Published: April 2024

Hawassa is a rapidly developing city in Lake Hawassa watershed of Ethiopia. Analyzing the effect of land cover dynamics on surface runoff remains imperative to adaptive urban stormwater management. This study quantified spatial variation of land cover and sensitivity of stormwater management response. Historical 30 years of daily annual rainfall, three satellite imageries, DEM, and hydrological soil group data were analyzed. A statistical‐based combined approach of geospatial techniques and Soil Conservation Service‐Curve Number (SCS‐CN) model was employed. CN and surface runoff depth for the delineated urban watersheds were determined. The result revealed that the built‐up area increased by 30.9 km2, where the rate varies spatially. The variation of impervious land cover explains 58.6% of change in CN with coefficient of 0.352. While CN is inversely correlated with agricultural and vegetation land cover variations. The finding suggests CN explains 96.78% of the change in surface runoff with a significant correlation coefficient of 3.91. The proposed integrated model approach justifies the potential to reorganize the relationship between the spatial effect of land cover variation on surface runoff at the urban watersheds. Thus, suitable local‐specific solutions can be devised for effective management of flood risk and optimize the drainage system of urban areas.
Microanatomy of the human tunnel of Corti structures and cochlear partition‐tonotopic variations and transcellular signaling
Authors: Giese Dina , Li Hao , Liu Wei , Staxäng Karin , Hodik Monika , Ladak Hanif M. , Agrawal Sumit , Schrott‐Fischer Anneliese , Glueckert Rudolf , Rask‐Andersen Helge
Published: April 2024

Auditory sensitivity and frequency resolution depend on the optimal transfer of sound‐induced vibrations from the basilar membrane (BM) to the inner hair cells (IHCs), the principal auditory receptors. There remains a paucity of information on how this is accomplished along the frequency range in the human cochlea. Most of the current knowledge is derived either from animal experiments or human tissue processed after death, offering limited structural preservation and optical resolution. In our study, we analyzed the cytoarchitecture of the human cochlear partition at different frequency locations using high‐resolution microscopy of uniquely preserved normal human tissue. The results may have clinical implications and increase our understanding of how frequency‐dependent acoustic vibrations are carried to human IHCs. A 1‐micron‐thick plastic‐embedded section (mid‐modiolar) from a normal human cochlea uniquely preserved at lateral skull base surgery was analyzed using light and transmission electron microscopy (LM, TEM). Frequency locations were estimated using synchrotron radiation phase‐contrast imaging (SR‐PCI). Archival human tissue prepared for scanning electron microscopy (SEM) and super‐resolution structured illumination microscopy (SR‐SIM) were also used and compared in this study. Microscopy demonstrated great variations in the dimension and architecture of the human cochlear partition along the frequency range. Pillar cell geometry was closely regulated and depended on the reticular lamina slope and tympanic lip angle. A type II collagen‐expressing lamina extended medially from the tympanic lip under the inner sulcus, here named “accessory basilar membrane.” It was linked to the tympanic lip and inner pillar foot, and it may contribute to the overall compliance of the cochlear partition. Based on the findings, we speculate on the remarkable microanatomic inflections and geometric relationships which relay different sound‐induced vibrations to the IHCs, including their relevance for the evolution of human speech reception and electric stimulation with auditory implants. The inner pillar transcellular microtubule/actin system's role of directly converting vibration energy to the IHC cuticular plate and ciliary bundle is highlighted.
Comparative skeletal anatomy of salt marsh and western harvest mice in relation to locomotor ecology
Authors: Woldt Kelsey M. , Pratt R. Brandon , Statham Mark J. , Barthman‐Thompson Laureen M. , Sustaita Diego
Published: April 2024

The salt marsh harvest mouse (Reithrodontomys raviventris) is an endangered species, endemic to the San Francisco Bay Estuary, that co‐occurs with the more broadly distributed species, the western harvest mouse (Reithrodontomys megalotis). Despite their considerable external morphological similarities, the northern subspecies of salt marsh harvest mice have relatively longer and thicker tails than do western harvest mice, which may be related to their abilities to climb emergent marsh vegetation to avoid tidal inundation. We used micro‐CT to compare post‐cranial skeletal anatomy between the salt marsh and western harvest mouse, to examine whether the salt marsh harvest mouse's restriction to brackish marshes is associated with skeletal adaptations for scansorial locomotion. We found that salt marsh harvest mice exhibited a deeper 3rd caudal vertebra, a more caudally located longest tail vertebra, craniocaudally longer tail vertebrae, and a longer digit III proximal phalanx than western harvest mice. These phalangeal and vertebral characteristics are known to decrease body rotations during climbing, increase contact with substrates, and decrease fall susceptibility in arboreal mammals, suggesting that the salt marsh harvest mouse may be morphologically specialized for scansorial locomotion, adaptive for its dynamic wetland environment.
A prospective study of patients with post treatment Lyme disease syndrome treated with modified VFEM energy
Authors: Derderian Gregory P. , Otenbaker Nicole
Published: April 2024

Background

We previously demonstrated a possible therapeutic benefit of VFEM (variable frequency electromagnetic energy) technology for the treatment of Post Treatment Lyme Disease Syndrome (PTLDS) or Chronic Lyme Disease (CLD). As a result, we prospectively enrolled 10 patients, all having significant debility, to determine to what extent we could improve their quality of life. Eight patients completed the 10 treatments.

Results

All eight patients had a significant improvement in quality of life within a 4‐month time frame.

Conclusion

VFEM is a stand‐alone modality that appears to demonstrate a significant improvement in quality of life in PTLDS or CLD with little or no risk or side effects of treatment.
Trichoscopic findings in children and adults with alopecia areata: A comparative study
Authors: Aryanian Zeinab , Najafi Morvarid , Ansari Mahshid , Nassimi Maryam , Etesami Ifa , Heidari Sama , Khayyat Azadeh , Hatami Parvaneh
Published: April 2024

Background

Alopecia areata is a chronic and relapsing condition that affects individuals of all age groups. Dermoscopy is a popular and non‐invasive method for diagnosing alopecia areata. This study aimed to analyze dermoscopic findings and their relationship with age, gender, appearance, and clinical signs in children and adults.

Methods

This retrospective cross‐sectional study was conducted on 124 children and adults diagnosed with alopecia areata who were referred to a Hospital in Iran between 2021 and 2022. After reaching the calculated sample size, trichoscopic findings were examined and the results were recorded. Data analysis was performed by a statistician and presented in relevant tables.

Results

The participants in the study comprised 53.2% female children, 46.8% male children, 27.42% adult males, and 72.58% adult females. The median age in the pediatric group was 10 years, while it was 27 years in the adult group. Yellow dots were significantly less observed in children than in adults (29% vs. 48.4%), while exclamation mark hairs were significantly more common in children than adults (38.7% vs. 21%). No significant differences were found in the frequency of other trichoscopic features between children and adults. Specifically, black dots, broken hairs, short vellus hairs, pigtail hairs, and empty follicular openings were observed in 38.7%, 40.3%, 32.3%, 11.3%, and 75.8% of children, respectively, and in 35.5%, 32.3%, 21%, 46.8%, and 12.9% of adults, respectively.

Conclusion

The most common trichoscopic findings in alopecia areata in children are empty follicular openings and broken hairs, while exclamation mark hairs are more common in children than adults. In contrast, yellow dots are less frequently observed in children compared to adults. This distinct difference between children's and adults' dermoscopic findings highlights the critical need for age‐specific considerations in AA evaluations.
The dynamic impact of trade on environment
Authors: Wang Huanhuan , Zhang Zhenglian , Zhang Zhiqiang
Published: April 2024

Utilizing long‐term and high‐resolution pollution remote sensing data, this study examines the dynamic impact of export expansion on the local environment in China from 2000 to 2020. Regions facing larger export expansion experience more pronounced initial deterioration in local air quality, while followed by a more prominent subsequent environmental improvement, indicating that the environmental impact of trade varies over time. Further analysis reveals that a key driver behind this dynamic change is the adjustment in environmental policies, particularly when local government faces higher pressure in achieving the environmental goals and when the central government takes over environmental monitoring powers. Additionally, under more stringent environmental regulations, regional export growth leads to increased environmental expenditures, concurrently driving localities to reduce heavily polluting engergy inputs such as coal. Our study suggests that environmental policies can facilitate the synergistic development of trade and the environment.
Semiotic whitening: Whiteness without white people
Authors: Mena Mike
Published: April 2024

In the borderlands of south Texas, the Mexican and Mexican American social practice of naming includes the use of English‐language names and nicknames, anglicized pronunciations, and English‐language spellings and “misspellings,” all of which potentially index at least two historically informed perspectives: (1) the hegemonic “white gaze”; and (2) a localized, interrogating gaze. In this article, I focus on local naming practices to advance an approach to what I call semiotic whitening—the indexical linking of any phenomenon to the idealized norms of whiteness—to better understand how whiteness works from the perspective of Mexicans and Mexican Americans living in a geographic region (informed by colonial and white supremacist histories) where few white folks reside.
Pseudomonas plecoglossicida(NR_114226) as a novel biocontrol agent against Fusarium crown rot of wheat
Authors: Makhlouf Kamel Eddine , Karima Boungab , Slimane Mokrani
Published: April 2024

Fusarium crown rot (FCR) poses a significant threat to wheat crops, causing substantial grain loss and mycotoxin contamination. While chemical fungicides have been effective in controlling this disease, the need for environmentally friendly alternatives is paramount due to concerns about human health and fungicide resistance. This study delves into the efficacy ofPseudomonas plecoglossicida(NR_114226) as a plant growth‐promoting and biocontrol agent againstFusarium graminearum(ON685926) andFusarium pseudograminearum(ON687723). Greenhouse experiments demonstrated a significant reduction in disease severity by 66.06% through seed treatment with this rhizobacterial strain in soil already infested with fungal pathogens. Moreover, significant growth enhancement occurred inF. pseudograminearum‐infected seedlings treated withP. plecoglossicida(F4 + P57), increasing plant height from 13.8 to 33.1 cm and root length from 9.56 to 19.62 cm, with notable improvements in fresh and dry weights. The study further validated these findings through enzymatic assays and analysis of synthesized secondary metabolites, including chitinase, protease, hydrocyanic acid, indole acetic acid, phosphate solubilization and siderophore production. These results strongly suggest thatP. plecoglossicidahas the potential to serve as an effective biocontrol agent against soilborne cereal pathogens.
First report Calonectria hawksworthii as an aetiological agent of fruit rot of cocoa in Brazil
Authors: de Melo Maruzanete Pereira , de Sousa Martins Sara , de Souza Soares Poliana Prates , Mesquita Naasoom Luiz Santos , Santos Armínio , de Novaes Quelmo Silva , Alves Vitória Cristina Santiago , Bezerra José Luiz , Bezerra Jadson Diogo Pereira
Published: April 2024

Cacao (Theobroma cacoa L.) is an important tropical fruit tree used in chocolate production. Among the biotic problems interfering with fruit production, phytopathogenic fungi stand out. During a visit to cocoa production areas in the municipality of Medicilândia, Pará, Brazil (Amazon region), we observed cocoa fruits with dark lesions and greyish‐white cottony mycelia growth. In order to determine the aetiological agent's identity, we extracted DNA and amplified part of the TEF‐1α and RBP2 genes and compared them with sequences in GenBank using the BLASTn tool. Based on molecular results and phylogenetic analysis, the isolates were identified as Calonectria hawksworthii. Inoculations in cacao fruits showed symptoms after 6 days and were similar to those collected in the field, confirming the postulates. This study is the first report of Ca. hawksworthii causing cocoa bean rot worldwide.
The effects of fermentation time on sourdough bread: An analysis of texture profile, starch digestion rate, and protein hydrolysis rate
Authors: Terrazas‐Avila Paulina , Palma‐Rodríguez Heidi M. , Navarro‐Cortez Ricardo O. , Hernández‐Uribe Juan P. , Piloni‐Martini Javier , Vargas‐Torres Apolonio
Published: April 2024

To ensure the best quality bread, it is important to consider the speed of digestion of starch and proteins, as well as how time fermentation and storage time influence the rate of starch digestion and the texture of the bread. This study compared the effect of fermentation time and days of storage on the texture, physicochemical, protein and starch digestibility of sourdough bread. Texture profile analysis showed that the fermentation time in recently baked sourdough bread affects hardness, chewiness, and springiness. The electrophoretic profile showed a decrease in band thickness with increase in fermentation time, consistent with a higher percentage of protein digestion. While fermentation time did not significantly affect rapidly digestible starch (RDS) and slowly digestible starch (SDS), storage time resulted in a decrease in RDS and an increase in SDS. Sourdough breads had higher levels of resistant starch (RS). The digestibility characteristics of protein and starch, as well as texture properties, are significantly influenced by fermentation and storage time. The evidence suggests that sourdough bread has the potential to improve the digestion of protein and to effectively regulate the glycemic response, which is due to its higher levels of SDS and RS.
Comparative study of the bronchodilator efficacy and adverse effects of salbutamol and hyoscine butylbromide in horses with severe asthma
Authors: Mozo Vives Berta , Mainguy‐Seers Sophie , Lavoie Jean‐Pierre
Published: April 2024

Background

Salbutamol and hyoscine butylbromide (HBB) are commonly used bronchodilators in horses with severe asthma (SA).

Objective

To compare the bronchodilation potency, duration, and adverse effects of salbutamol and HBB in SA.

Animals

Six horses in exacerbation of SA.

Methods

The effects of inhaled salbutamol (1000 μg) and HBB (150 mg, IV) were compared in a randomized, blinded, crossover experiment. Lung function, intestinal borborygmi and heart rate were assessed before and sequentially until 180 minutes after drug administration, and analyzed with 2‐way repeated‐measures ANOVA and Dunnett's multiple comparison tests.

Results

Both treatments caused a similar improvement in lung function. Pulmonary resistance and reactance returned to baseline values within 30 minutes after HBB administration, whereas salbutamol improved reactance until 180 minutes (mean improvement at 180 minutes of 0.040 Kpa/L/s, 95% CI = 0.004 to 0.076; P = .02 for salbutamol and of 0.009 Kpa/L/s, 95% CI = −0.028 to 0.045; P = .98 for HBB for the resistance at 3 Hz and of 0.040 Kpa/L/s, 95% CI = 0.007 to 0.074; P = .01 for salbutamol and of 0.009 Kpa/L/s, 95% CI = −0.024 to 0.042; P = .97 for HBB for the reactance at 7 Hz). From 5 to 30 minutes after HBB administration, the heart rate accelerated (mean increase of 3.3 beats per minute, 95% CI = −6.6 to 13.1; P = .92 for salbutamol, and of 13.0 beats per minute, 95% CI = 3.6 to 22.4; P = .002 for HBB at 30 minutes) and the gut sounds decreased (mean reduction of 1.3, 95% CI = −0.1 to 2.8; P = .09 for salbutamol and of 2.8 for the gastrointestinal auscultation score, 95% CI = 1.4 to 4.3; P < .0001 for HBB at 30 minutes).

Conclusions and Clinical Importance

Both drugs have a similar bronchodilator potency but with a longer duration for salbutamol. Gastrointestinal and cardiovascular effects were noted only with HBB, suggesting the preferential use of salbutamol to relieve bronchoconstriction in horses with asthma.
Safety profile and effects on the peripheral immune response of fecal microbiota transplantation in clinically healthy dogs
Authors: Lee Mary Ann , Questa Maria , Wanakumjorn Patrawin , Kol Amir , McLaughlin Bridget , Weimer Bart C. , Buono Agostino , Suchodolski Jan S. , Marsilio Sina
Published: April 2024

Background

Fecal microbiota transplantation (FMT) is increasingly used for gastrointestinal and extra‐gastrointestinal diseases in veterinary medicine. However, its effects on immune responses and possible adverse events have not been systematically investigated.

Hypothesis/Objectives

Determine the short‐term safety profile and changes in the peripheral immune system after a single FMT administration in healthy dogs.

Animals

Ten client‐owned, clinically healthy dogs as FMT recipients, and 2 client‐owned clinically healthy dogs as FMT donors.

Methods

Prospective non‐randomized clinical trial. A single rectal enema of 5 g/kg was given to clinically healthy canine recipients. During the 28 days after FMT administration, owners self‐reported adverse events and fecal scores. On Days 0 (baseline), 1, 4, 10, and 28 after FMT, fecal and blood samples were collected. The canine fecal dysbiosis index (DI) was calculated using qPCR.

Results

No significant changes were found in the following variables: CBC, serum biochemistry, C‐reactive protein, serum cytokines (interleukins [IL]‐2, ‐6, ‐8, tumor necrosis factor [TNF]‐α), peripheral leukocytes (B cells, T cells, cluster of differentiation [CD]4+ T cells, CD8+ T cells, T regulatory cells), and the canine DI. Mild vomiting (n = 3), diarrhea (n = 4), decreased activity (n = 2), and inappetence (n = 1) were reported, and resolved without intervention.

Conclusions and Clinical Importance

Fecal microbiota transplantation did not significantly alter the evaluated variables and recipients experienced minimal adverse events associated with FMT administration. Fecal microbiota transplantation was not associated with serious adverse events, changes in peripheral immunologic variables, or the canine DI in the short‐term.
Equine neuroaxonal dystrophy/degenerative myeloencephalopathy in Gypsy Vanner horses
Authors: Powers Alexis , Peek Simon F. , Reed Steve , Donnelly Callum G. , Tinkler Stacey , Gasper David , Woolard Kevin D. , Finno Carrie J.
Published: April 2024

Background

Equine neuroaxonal dystrophy/degenerative myeloencephalopathy (eNAD/EDM) is a neurodegenerative disease that primarily affects young, genetically predisposed horses that are deficient in vitamin E. Equine NAD/EDM has not previously been documented in Gypsy Vanner horses (GVs).

Objectives

To evaluate: (1) the clinical phenotype, blood vitamin E concentrations before and after supplementation and pedigree in a cohort of GV horses with a high prevalence of neurologic disease suspicious for eNAD/EDM and (2) to confirm eNAD/EDM in GVs through postmortem evaluation.

Animals

Twenty‐six GVs from 1 farm in California and 2 cases from the Midwestern U.S.

Methods

Prospective observational study on Californian horses; all 26 GVs underwent neurologic examination. Pre‐supplementation blood vitamin E concentration was assessed in 17‐ GVs. Twenty‐three were supplemented orally with 10 IU/kg of liquid RRR‐alpha‐tocopherol once daily for 28 days. Vitamin E concentration was measured in 23 GVs after supplementation, of which 15 (65%) had pre‐supplementation measurements. Two clinically affected GVs from California and the 2 Midwestern cases had necropsy confirmation of eNAD/EDM.

Results

Pre‐supplementation blood vitamin E concentration was ≤2.0 μg/mL in 16/17 (94%) of GVs from California. Post‐supplementation concentration varied, with a median of 3.39 μg/mL (range, 1.23‐13.87 μg/mL), but only 12/23 (52%) were normal (≥3.0 μg/mL). Normalization of vitamin E was significantly associated with increasing age (P = .02). Euthanized horses (n = 4) had eNAD/EDM confirmed at necropsy.

Conclusions and Clinical Importance

GVs could have a genetic predisposition to eNAD/EDM. Vitamin E supplementation should be considered and monitored in young GVs.