bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Kobe-type and Otsu-typeBabesia microtiin wild rodents in China's Yunnan province
Authors: CHEN XIN-RONG , YE LI , FAN JUN-WEN , LI CHANG , TANG FANG , LIU WEI , REN LIN-ZHU , BAI JIE-YING
Published: August 2017

Babesiosis is an emerging tick-transmitted zoonosis prevalent in large parts of the world. This study was designed to determine the rates ofBabesia microtiinfection among small rodents in Yunnan province, where human cases of babesiosis have been reported. Currently, distribution ofBabesiain its endemic regions is largely unknown. In this study, we cataloged 1672 small wild rodents, comprising 4 orders, from nine areas in western Yunnan province between 2009 and 2011.Babesia microtiDNA was detected by polymerase chain reaction in 4·3% (72/1672) of the rodents analyzed. The most frequently infected rodent species includedApodemus chevrieriandNiviventer fulvescens. Rodents from forests and shrublands had significantly higherBabesiainfection rates. Genetic comparisons revealed thatBabesiawas most similar to the Kobe- and Otsu-type strains identified in Japan. A variety of rodent species might be involved in the enzootic maintenance and transmission ofB. microti, supporting the need for further serological investigations in humans.
Organization of the dorsal lateral geniculate nucleus in the mouse
Authors: KERSCHENSTEINER DANIEL , GUIDO WILLIAM
Published: August 2017

The dorsal lateral geniculate nucleus (dLGN) of the thalamus is the principal conduit for visual information from retina to visual cortex. Viewed initially as a simple relay, recent studies in the mouse reveal far greater complexity in the way input from the retina is combined, transmitted, and processed in dLGN. Here we consider the structural and functional organization of the mouse retinogeniculate pathway by examining the patterns of retinal projections to dLGN and how they converge onto thalamocortical neurons to shape the flow of visual information to visual cortex.
Learning opacity in Stratal Maximum Entropy Grammar
Authors: Nazarov Aleksei , Pater Joe
Published: August 2017

Opaque phonological patterns are sometimes claimed to be difficult to learn; specific hypotheses have been advanced about the relative difficulty of particular kinds of opaque processes (Kiparsky 1971, 1973), and the kind of data that is helpful in learning an opaque pattern (Kiparsky 2000). In this paper, we present a computationally implemented learning theory for one grammatical theory of opacity, a Maximum Entropy version of Stratal OT (Bermúdez-Otero 1999, Kiparsky 2000), and test it on simplified versions of opaque French tense–lax vowel alternations and the opaque interaction of diphthong raising and flapping in Canadian English. We find that the difficulty of opacity can be influenced by evidence for stratal affiliation: the Canadian English case is easier if the learner encounters application of raising outside the flapping context, or non-application of raising between words (e.g. life with [ʌɪ]; lie for with [aɪ]).
Brain alterations potentially associated with aggression and terrorism
Authors: Bogerts Bernhard , Schöne Maria , Breitschuh Stephanie
Published: August 2017

A large proportion of the persons who join terrorist groups as well as lone-acting terrorists have a history of violent behavior or mental disorder that predated their becoming terrorists. This suggests that brain alterations found to occur in violent perpetrators may also be present in a significant percentage of terrorists. After a short delineation of phylogenetically old neuronal networks that are important for the generation of aggressive behavior in inconspicuous brains, this review summarizes structural and functional brain-imaging studies in violent offenders published over the last 10 years. Depending on the subtype of violence (impulsive or instrumental), deviations in structure or function were mainly found in the prefrontal, orbitofrontal, and insular cortex, as well as in temporolimbic structures (e.g., the amygdala, hippocampus, and parahippocampus). These brain areas are essentially responsible for the control of the archaic neuronal generators of aggression located in the hypothalamus and limbic system. This regional distribution of brain alterations also shows a remarkable overlap with those brain regions that are crucial for such prosocial traits as empathy and compassion. Feelings of superiority, dominance, and satisfaction gained by performing violent and terroristic attacks suggest that a hedonistic component via an activation of brain reward systems plays an additional role. In our current debate about the causes of terrorism, aspects of brain dysfunction should receive more attention.
Vitamin D intake and risk of CVD and all-cause mortality: evidence from the Caerphilly Prospective Cohort Study
Authors: Guo Jing , Cockcroft John R , Elwood Peter C , Pickering Janet E , Lovegrove Julie A , Givens David I
Published: August 2017

Objective

Prospective data on the associations between vitamin D intake and risk of CVD and all-cause mortality are limited and inconclusive. The aim of the present study was to investigate the associations between vitamin D intake and CVD risk and all-cause mortality in the Caerphilly Prospective Cohort Study.

Design

The associations of vitamin D intake with CVD risk markers were examined cross-sectionally at baseline and longitudinally at 5-year, 10-year and >20-year follow-ups. In addition, the predictive value of vitamin D intake for CVD events and all-cause mortality after >20 years of follow-up was examined. Logistic regression and general linear regression were used for data analysis.

Setting

Participants in the UK.

Subjects

Men (n452) who were free from CVD and type 2 diabetes at recruitment.

Results

Higher vitamin D intake was associated with increased HDL cholesterol (P=0·003) and pulse pressure (P=0·04) and decreased total cholesterol:HDL cholesterol (P=0·008) cross-sectionally at baseline, but the associations were lost during follow-up. Furthermore, higher vitamin D intake was associated with decreased concentration of plasma TAG at baseline (P=0·01) and at the 5-year (P=0·01), but not the 10-year examination. After >20 years of follow-up, vitamin D was not associated with stroke (n72), myocardial infarctions (n142), heart failure (n43) or all-cause mortality (n281), but was positively associated with increased diastolic blood pressure (P=0·03).

Conclusions

The study supports associations of higher vitamin D intake with lower fasting plasma TAG and higher diastolic blood pressure.
The school environment and sugar-sweetened beverage consumption among Guatemalan adolescents
Authors: Godin Katelyn M , Chacón Violeta , Barnoya Joaquin , Leatherdale Scott T
Published: August 2017

Objective

The current study sought to examine Guatemalan adolescents’ consumption of sugar-sweetened beverages (SSB), identify which individual-level characteristics are associated with SSB consumption and describe school characteristics that may influence students’ SSB consumption.

Design

Within this observational pilot study, a questionnaire was used to assess students’ consumption of three varieties of SSB (soft drinks, energy drinks, sweetened coffees/teas), as well as a variety of sociodemographic and behavioural characteristics. We collected built environment data to examine aspects of the school food environment. We developed Poisson regression models for each SSB variety and used descriptive analyses to characterize the sample.

Setting

Guatemala City, Guatemala.

Subjects

Guatemalan adolescents (n1042) from four (two public, two private) secondary schools.

Results

Built environment data revealed that students from the two public schools lacked access to water fountains/coolers. The SSB industry had a presence in the schools through advertisements, sponsored food kiosks and products available for sale. Common correlates of SSB consumption included school type, sedentary behaviour, frequency of purchasing lunch in the cafeteria, and frequency of purchasing snacks from vending machines in school and off school property.

Conclusions

Guatemalan adolescents frequently consume SSB, which may be encouraged by aspects of the school environment. Schools represent a viable setting for equitable population health interventions designed to reduce SSB consumption, including increasing access to clean drinking-water, reducing access to SSB, restricting SSB marketing and greater enforcement of existing food policies.
REFLECTORS AND GLOBALIZATIONS OF PARTIAL ACTIONS OF GROUPS
Authors: KHRYPCHENKO MYKOLA , NOVIKOV BORIS
Published: August 2017

Given a partial action $\unicode[STIX]{x1D703}$ of a group on a set with an algebraic structure, we construct a reflector of $\unicode[STIX]{x1D703}$ in the corresponding subcategory of global actions and study the question when this reflector is a globalization. In particular, if $\unicode[STIX]{x1D703}$ is a partial action on an algebra from a variety $\mathsf{V}$, then we show that the problem reduces to the embeddability of a certain generalized amalgam of $\mathsf{V}$-algebras associated with $\unicode[STIX]{x1D703}$. As an application, we describe globalizable partial actions on semigroups, whose domains are ideals.
A VARIANT OF HARISH-CHANDRA FUNCTORS
Authors: Crisp Tyrone , Meir Ehud , Onn Uri
Published: August 2017

Harish-Chandra induction and restriction functors play a key role in the representation theory of reductive groups over finite fields. In this paper, extending earlier work of Dat, we introduce and study generalisations of these functors which apply to a wide range of finite and profinite groups, typical examples being compact open subgroups of reductive groups over non-archimedean local fields. We prove that these generalisations are compatible with two of the tools commonly used to study the (smooth, complex) representations of such groups, namely Clifford theory and the orbit method. As a test case, we examine in detail the induction and restriction of representations from and to the Siegel Levi subgroup of the symplectic group $\text{Sp}_{4}$ over a finite local principal ideal ring of length two. We obtain in this case a Mackey-type formula for the composition of these induction and restriction functors which is a perfect analogue of the well-known formula for the composition of Harish-Chandra functors. In a different direction, we study representations of the Iwahori subgroup $I_{n}$ of $\text{GL}_{n}(F)$, where $F$ is a non-archimedean local field. We establish a bijection between the set of irreducible representations of $I_{n}$ and tuples of primitive irreducible representations of smaller Iwahori subgroups, where primitivity is defined by the vanishing of suitable restriction functors.
Cutting management and alfalfa stand age effects on organically grown corn grain yield and soil N availability
Authors: Fernandez Adria L. , Fabrizzi Karina P. , Tautges Nicole E. , Lamb John A. , Sheaffer Craig C.
Published: August 2017

Alfalfa is recommended as a rotational crop in corn production, due to its ability to contribute to soil nitrogen (N) and carbon (C) stocks through atmospheric N2fixation and above- and belowground biomass production. However, there is little information on how alfalfa management practices affect contributions to soil and subsequent corn crop yields, and research has not been targeted to organic systems. A study was conducted to determine the effects of alfalfa stand age, cutting frequency and biomass removal on soil C and N status and corn yields at three organically managed Minnesota locations. In one experiment, five cutting treatments were applied in nine environments: two, three and four cuts with biomass removal; three cuts with biomass remaining in place; and a no-cut control. In the other experiment, corn was planted following 1-, 2-, 3- or 4-year-old alfalfa stands and a no-alfalfa control. Yield was measured in the subsequent corn crop. In the cutting experiment, the two- and three-cut treatments with biomass removal reduced soil mineral N by 12.6 and 11.5%, respectively, compared with the control. Potentially mineralizable N (PMN) was not generally affected by cutting treatments. The three-cut no-removal increased potentially mineralizable C by 17% compared with the other treatments, but lowered soil total C in two environments, suggesting a priming effect in which addition of alfalfa biomass stimulated microbial mineralization of native soil C. Although both yields and soil mineral N tended to be higher in treatments where biomass remained in place, this advantage was small and inconsistent, indicating that farmers need not forgo hay harvest to obtain the rotational benefits of an alfalfa stand. The lack of overall correlation between corn grain yields and mineral and potentially mineralizable N suggests that alfalfa N contribution was not the driver of the yield increase in the no-removal treatments. Alfalfa stand age had inconsistent effects on fall-incorporated N and soil N and C parameters. Beyond the first year, increased alfalfa stand age did not increase soil mineral N or PMN. However, corn yield increased following older stands. Yields were 29, 77 and 90% higher following first-, second- and third-year alfalfa stands than the no-alfalfa control, respectively. This indicates that alfalfa may benefit succeeding corn through mechanisms other than N contribution, potentially including P solubilization and weed suppression. These effects have been less studied than N credits, but are of high value in organic cropping systems.
Socioeconomic position and depression in South African adults with long-term health conditions: a longitudinal study of causal pathways
Authors: Elwell-Sutton T. , Folb N. , Clark A. , Fairall L. R. , Lund C. , Bachmann M. O.
Published: August 2017

Aims.

There is convincing evidence that lower socioeconomic position is associated with increased risk of mental disorders. However, the mechanisms involved are not well understood. This study aims to elucidate the causal pathways between socioeconomic position and depression symptoms in South African adults. Two possible causal theories are examined: social causation, which suggests that poor socioeconomic conditions cause mental ill health; and social drift, which suggests that those with poor mental health are more likely to drift into poor socioeconomic circumstances.

Methods.

The study used longitudinal and cross-sectional observational data on 3904 adults, from a randomised trial carried out in 38 primary health care clinics between 2011 and 2012. Structural equation models and counterfactual mediation analyses were used to examine causal pathways in two directions. First, we examined social causation pathways, with language (a proxy for racial or ethnic category) being treated as an exposure, while education, unemployment, income and depression were treated as sequential mediators and outcomes. Second, social drift was explored with depression treated as a potential influence on health-related quality of life, job loss and, finally, income.

Results.

The results suggest that the effects of language on depression at baseline, and on changes in depression during follow-up, were mediated through education and income but not through unemployment. Adverse effects of unemployment and job loss on depression appeared to be mostly mediated through income. The effect of depression on decreasing income appeared to be mediated by job loss.

Conclusions.

These results suggest that both social causation and social selection processes operate concurrently. This raises the possibility that people could get trapped in a vicious cycle in which poor socioeconomic conditions lead to depression, which, in turn, can cause further damage to their economic prospects. This study also suggests that modifiable factors such as income, employment and treatable depression are suitable targets for intervention in the short to medium term, while in the longer term reducing inequalities in education will be necessary to address the deeply entrenched inequalities in South Africa.
Nuclear matrix metalloproteinases: functions resemble the evolution from the intracellular to the extracellular compartment
Authors: Xie Yingqiu , Mustafa Aidana , Yerzhan Adina , Merzhakupova Dalmira , Yerlan Perizat , N Orakov Askarbek , Wang Xiao , Huang Yi , Miao Lixia
Published: August 2017

Matrix metalloproteinase (MMP) is defined as an endopeptidase in the extracellular matrix (ECM), which plays essential roles in physiological processes such as organogenesis, wound healing, angiogenesis, apoptosis and motility. MMPs are produced and assembled in the cytoplasm as proenzymes with a cytoplasmic domain and require extracellular activation. MMPs can degrade receptors, extracellular matrix proteins, PARPs and release apoptotic substances. MMPs have been found in the cytosol, organelles and extracellular compartments and recently many types of MMPs have been found in the nucleus. However, the mechanisms and roles of MMPs inside the cell nucleus are still poorly understood. Here we summarized the nuclear localization mechanisms of MMPs and their functions in the nucleus such as apoptosis, tissue remodeling upon injury and cancer progression. Most importantly, we found that nuclear MMPs have evolved to translocate to membrane and target ECM possibly through evolution of nuclear localization signal (NLS), natural selection and anti-apoptotic survival. Thus, the knowledge about the evolution and regulation of nuclear MMPs appears to be essential in understanding a variety of cellular processes along with the development of MMP-targeted therapeutic drugs against the progression of certain diseases.
Fermion-induced quantum critical points
Authors: Li Zi-Xiang , Jiang Yi-Fan , Jian Shao-Kai , Yao Hong
Published: August 2017

A unified theory of quantum critical points beyond the conventional Landau–Ginzburg–Wilson paradigm remains unknown. According to Landau cubic criterion, phase transitions should be first-order when cubic terms of order parameters are allowed by symmetry in the Landau–Ginzburg free energy. Here, from renormalization group analysis, we show that second-order quantum phase transitions can occur at such putatively first-order transitions in interacting two-dimensional Dirac semimetals. As such type of Landau-forbidden quantum critical points are induced by gapless fermions, we call them fermion-induced quantum critical points. We further introduce a microscopic model of SU(N) fermions on the honeycomb lattice featuring a transition between Dirac semimetals and Kekule valence bond solids. Remarkably, our large-scale sign-problem-free Majorana quantum Monte Carlo simulations show convincing evidences of a fermion-induced quantum critical points for N = 2, 3, 4, 5 and 6, consistent with the renormalization group analysis. We finally discuss possible experimental realizations of the fermion-induced quantum critical points in graphene and graphene-like materials.
Solution nuclear magnetic resonance spectroscopy on a nanostructured diamond chip
Authors: Kehayias P. , Jarmola A. , Mosavian N. , Fescenko I. , Benito F. M. , Laraoui A. , Smits J. , Bougas L. , Budker D. , Neumann A. , Brueck S. R. J. , Acosta V. M.
Published: August 2017

Sensors using nitrogen-vacancy centers in diamond are a promising tool for small-volume nuclear magnetic resonance (NMR) spectroscopy, but the limited sensitivity remains a challenge. Here we show nearly two orders of magnitude improvement in concentration sensitivity over previous nitrogen-vacancy and picoliter NMR studies. We demonstrate NMR spectroscopy of picoliter-volume solutions using a nanostructured diamond chip with dense, high-aspect-ratio nanogratings, enhancing the surface area by 15 times. The nanograting sidewalls are doped with nitrogen-vacancies located a few nanometers from the diamond surface to detect the NMR spectrum of roughly 1 pl of fluid lying within adjacent nanograting grooves. We perform 1H and 19F nuclear magnetic resonance spectroscopy at room temperature in magnetic fields below 50 mT. Using a solution of CsF in glycerol, we determine that 4 ± 2 × 1012 19F spins in a 1 pl volume can be detected with a signal-to-noise ratio of 3 in 1 s of integration.
Exit from quiescence displays a memory of cell growth and division
Authors: Wang Xia , Fujimaki Kotaro , Mitchell Geoffrey C. , Kwon Jungeun Sarah , Della Croce Kimiko , Langsdorf Chris , Zhang Hao Helen , Yao Guang
Published: August 2017

Reactivating quiescent cells to proliferate is critical to tissue repair and homoeostasis. Quiescence exit is highly noisy even for genetically identical cells under the same environmental conditions. Deregulation of quiescence exit is associated with many diseases, but cellular mechanisms underlying the noisy process of exiting quiescence are poorly understood. Here we show that the heterogeneity of quiescence exit reflects a memory of preceding cell growth at quiescence induction and immediate division history before quiescence entry, and that such a memory is reflected in cell size at a coarse scale. The deterministic memory effects of preceding cell cycle, coupled with the stochastic dynamics of an Rb-E2F bistable switch, jointly and quantitatively explain quiescence-exit heterogeneity. As such, quiescence can be defined as a distinct state outside of the cell cycle while displaying a sequential cell order reflecting preceding cell growth and division variations.
Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity
Authors: Loffreda Alessia , Jacchetti Emanuela , Antunes Sofia , Rainone Paolo , Daniele Tiziana , Morisaki Tatsuya , Bianchi Marco E. , Tacchetti Carlo , Mazza Davide
Published: August 2017

Live-cell microscopy has highlighted that transcription factors bind transiently to chromatin but it is not clear if the duration of these binding interactions can be modulated in response to an activation stimulus, and if such modulation can be controlled by post-translational modifications of the transcription factor. We address this question for the tumor suppressor p53 by combining live-cell single-molecule microscopy and single cell in situ measurements of transcription and we show that p53-binding kinetics are modulated following genotoxic stress. The modulation of p53 residence times on chromatin requires C-terminal acetylation—a classical mark for transcriptionally active p53—and correlates with the induction of transcription of target genes such as CDKN1a. We propose a model in which the modification state of the transcription factor determines the coupling between transcription factor abundance and transcriptional activity by tuning the transcription factor residence time on target sites.
GADD45A plays a protective role against temozolomide treatment in glioblastoma cells
Authors: Wang Hsiao-Han , Chang Tsuey-Yu , Lin Wei-Chen , Wei Kuo-Chen , Shin Jyh-Wei
Published: August 2017

Glioblastoma multiforme (GBM) is one of the most aggressive cancers. Despite recent advances in multimodal therapies, high-grade glioma remains fatal. Temozolomide (TMZ) is an alkylating agent used worldwide for the clinical treatment of GBM; however, the innate and acquired resistance of GBM limits its application. Here, we found that TMZ inhibited the proliferation and induced the G2/M arrest of GBM cells. Therefore, we performed microarrays to identify the cell cycle- and apoptosis-related genes affected by TMZ. Notably, GADD45A was found to be up-regulated by TMZ in both cell cycle and apoptosis arrays. Furthermore, GADD45A knockdown (GADD45Akd) enhanced the cell growth arrest and cell death induced by TMZ, even in natural (T98) and adapted (TR-U373) TMZ-resistant cells. Interestingly, GADD45Akd decreased the expression of O6-methylguanine-DNA methyltransferase (MGMT) in TMZ-resistant cells (T98 and TR-U373). In MGMT-deficient/TMZ-sensitive cells (U87 and U373), GADD45Akd decreased TMZ-induced TP53 expression. Thus, in this study, we investigated the genes influenced by TMZ that were important in GBM therapy, and revealed that GADD45A plays a protective role against TMZ treatment which may through TP53-dependent and MGMT-dependent pathway in TMZ-sensitive and TMZ-resistant GBM, respectively. This protective role of GADD45A against TMZ treatment may provide a new therapeutic strategy for GBM treatment.
Assessment of the announced North Korean nuclear test using long-range atmospheric transport and dispersion modelling
Authors: De Meutter Pieter , Camps Johan , Delcloo Andy , Termonia Piet
Published: August 2017

On 6 January 2016, the Democratic People’s Republic of Korea announced to have conducted its fourth nuclear test. Analysis of the corresponding seismic waves from the Punggye-ri nuclear test site showed indeed that an underground man-made explosion took place, although the nuclear origin of the explosion needs confirmation. Seven weeks after the announced nuclear test, radioactive xenon was observed in Japan by a noble gas measurement station of the International Monitoring System. In this paper, atmospheric transport modelling is used to show that the measured radioactive xenon is compatible with a delayed release from the Punggye-ri nuclear test site. An uncertainty quantification on the modelling results is given by using the ensemble method. The latter is important for policy makers and helps advance data fusion, where different nuclear Test-Ban-Treaty monitoring techniques are combined.
GRACE satellite observations reveal the severity of recent water over-consumption in the United States
Authors: Solander Kurt C. , Reager John T. , Wada Yoshihide , Famiglietti James S. , Middleton Richard S.
Published: August 2017

Changes in the climate and population growth will critically impact the future supply and demand of water, leading to large uncertainties for sustainable resource management. In the absence of on-the-ground measurements to provide spatially continuous, high-resolution information on water supplies, satellite observations can provide essential insight. Here, we develop a technique using observations from the Gravity Recovery and Climate Experiment (GRACE) satellite to evaluate the sustainability of surface water and groundwater use over the continental United States. We determine the annual total water availability for 2003–2015 using the annual variability in GRACE-derived total water storage for 18 major watersheds. The long-term sustainable water quantity available to humans is calculated by subtracting an annual estimate of the water needed to maintain local ecosystems, and the resulting water volumes are compared to reported consumptive water use to determine a sustainability fraction. We find over-consumption is highest in the southwest US, where increasing stress trends were observed in all five basins and annual consumptive use exceeded 100% availability twice in the Lower Colorado basin during 2003–2015. By providing a coarse-scale evaluation of sustainable water use from satellite and ground observations, the established framework serves as a blueprint for future large-scale water resource monitoring.
Bioactivity-Guided Fractionation of Pine Needle Reveals Catechin as an Anti-hypertension Agent via Inhibiting Angiotensin-Converting Enzyme
Authors: He Jian
Published: August 2017

Hypertension has been recognized as one of the highest risk factors for cardiovascular diseases. Anti-hypertension agent screening and development has been recognized as a pharmaceutical therapy approach for the cardiovascular diseases treatment. Many kinds of traditional Chinese medicines, such as pine needle, have been used for the treatment of hypertension for a long time, but the bioactive ingredients which responsible for their therapeutic effectiveness are remain unclear. Therefore, screening bioactive chemicals in natural sources is still the most straightforward strategy for novel Angiotensin-converting enzyme inhibitor (ACEi)-based anti-hypertension agents discovery. In this study, we demonstrated a bioactivity-guided fractionation strategy for identifying bioactive fractions and chemicals from pine needle based on LC/MS assay as well as elucidating their mechanisms of pharmacological activity. And we found out the compound in pine needle extracts being ACE-inhibitory active is catechin. When ACE activity was assayed in rat tissue membranes, it was observed that catechin demonstrate ACE inhibition in kidney, lung and testes tissue. All these presents catechin in pine needle could be a potential cardiovascular medicine.
Neural, physiological, and behavioral correlates of visuomotor cognitive load
Authors: Hosseini S. M. Hadi , Bruno Jennifer L. , Baker Joseph M. , Gundran Andrew , Harbott Lene K. , Gerdes J. Christian , Reiss Allan L.
Published: August 2017

Visuomotor ability is quite crucial for everyday functioning, particularly in driving and sports. While there is accumulating evidence regarding neural correlates of visuomotor transformation, less is known about the brain regions that accommodate visuomotor mapping under different cognitive demands. We concurrently measured cortical activity and pupillary response, using functional near infrared spectroscopy (fNIRS) and eye-tracking glasses, to examine the neural systems linked to pupil dilation under varying cognitive demands. Twenty-three healthy adults performed two sessions of a navigation task, in which the cognitive load was manipulated by either reversing the visuomotor mapping or increasing the speed of the moving object. We identified a region in the right superior parietal lobule that responded to both types of visuomotor load and its activity was associated with larger pupillary response and better performance in the task. Our multimodal analyses suggest that activity in this region arises from the need for increased attentional effort and alertness for visuomotor control and is an ideal candidate for objective measurement of visuomotor cognitive load. Our data extend previous findings connecting changes in pupil diameter to neural activity under varying cognitive demand and have important implications for examining brain-behavior associations in real-world tasks such as driving and sports.
Infiltration of M1, but not M2, macrophages is impaired after unilateral ureter obstruction in Nrf2-deficient mice
Authors: Sogawa Yuji , Nagasu Hajime , Iwase Shigeki , Ihoriya Chieko , Itano Seiji , Uchida Atsushi , Kidokoro Kengo , Taniguchi Shun’ichiro , Takahashi Masafumi , Satoh Minoru , Sasaki Tamaki , Suzuki Takafumi , Yamamoto Masayuki , Horng Tiffany , Kashihara Naoki
Published: August 2017

Chronic inflammation can be a major driver of the failure of a variety of organs, including chronic kidney disease (CKD). The NLR family pyrin domain-containing 3 (NLRP3) inflammasome has been shown to play a pivotal role in inflammation in a mouse kidney disease model. Nuclear factor erythroid 2-related factor 2 (Nrf2), the master transcription factor for anti-oxidant responses, has also been implicated in inflammasome activation under physiological conditions. However, the mechanism underlying inflammasome activation in CKD remains elusive. Here, we show that the loss of Nrf2 suppresses fibrosis and inflammation in a unilateral ureter obstruction (UUO) model of CKD in mice. We consistently observed decreased expression of inflammation-related genes NLRP3 and IL-1β in Nrf2-deficient kidneys after UUO. Increased infiltration of M1, but not M2, macrophages appears to mediate the suppression of UUO-induced CKD symptoms. Furthermore, we found that activation of the NLRP3 inflammasome is attenuated in Nrf2-deficient bone marrow–derived macrophages. These results demonstrate that Nrf2-related inflammasome activation can promote CKD symptoms via infiltration of M1 macrophages. Thus, we have identified the Nrf2 pathway as a promising therapeutic target for CKD.
The integration of Gβ and MAPK signaling cascade in zygote development
Authors: Yuan Guo-Liang , Li Hong-Ju , Yang Wei-Cai
Published: August 2017

Cells respond to many signals with a limited number of signaling components. Heterotrimeric G proteins and MAPK cascades are universally used by eukaryotic cells to transduce signals in various developmental processes or stress responses by activating different effectors. MAPK cascade is integrated with G proteins by scaffold protein during plant immunity. However, the molecular relationship between G proteins and MAPK modules in plant development is still unclear. In this study, we demonstrate that Arabidopsis Gβ protein AGB1 interacts with MPK3 and 6, MKK4 and 5, as well as the regulatory domains of YODA (YDA), the upstream MEKK of MKK4/5. Remarkably, YDA interacts with the plasma membrane associated SHORT SUSPENSOR (SSP) through its N- and C-terminal region in vitro and in vivo. Additionally, genetic analysis shows that AGB1 functions together with MPK3/6 signaling cascade during the asymmetric division of the zygote. These data indicate that Gβ may function likely as a scaffold, through direct physical interaction with the components of the MPK signaling module in plant development. Our results provide new insights into the molecular functions of G protein and will advance the understanding of the complex mechanism of kinase signaling cascades.
Effect of the land area elevation on the collective choice in ants
Authors: Bles Olivier , Lozet Nathanaël , de Biseau Jean-Christophe , Campo Alexandre , Deneubourg Jean-Louis
Published: August 2017

Collective decisions regarding food source exploitation in social insects are influenced by a range of parameters, from source quality to individual preference and social information sharing. Those regarding the elevation of the physical trail towards a food source have been neglected. In this work, we investigated the effect of ascending and descending paths from the nest to a food source on collective choice in two ant species Lasius niger and Myrmica rubra. Our hypothesis that returning loaded with food from the high source is more energy efficient was validated by choice experiments: when the sources are simultaneously introduced the high food source is preferentially exploited by both species. The flexibility of colony response was then tested by introducing the preferred source (high) incidentally, after recruitment towards the down food source began. Despite the well-known lack of flexibility of L. niger, both species showed the ability to reallocate their foraging workforce towards the highest food source. The collective choice and the flexibility are based on the difference between the u-turn rates when foragers are facing the ascending or descending branch. We discuss these results in terms of species-specifics characteristics and ecological context.
Structure sensitive photocatalytic reduction of nitroarenes over TiO
2
Authors: Challagulla Swapna , Tarafder Kartick , Ganesan Ramakrishnan , Roy Sounak
Published: August 2017

It is a subject of exploration whether the phase pure anatase or rutile TiO2 or the band alignment due to the heterojunctions in the two polymorphs of TiO2 plays the determining role in efficacy of a photocatalytic reaction. In this work, the phase pure anatase and rutile TiO2 have been explored for photocatalytic nitroarenes reduction to understand the role of surface structures and band alignment towards the reduction mechanism. The conduction band of synthesized anatase TiO2 has been found to be more populated with electrons of higher energy than that of synthesized rutile. This has given the anatase an edge towards photocatalytic reduction of nitroarenes over rutile TiO2. The other factors like adsorption of the reactants and the proton generation did not play any decisive role in catalytic efficacy.
Light color importance for circadian entrainment in a diurnal (Octodon degus) and a nocturnal (Rattus norvegicus) rodent
Authors: Bonmati-Carrion Maria Angeles , Baño-Otalora Beatriz , Madrid Juan Antonio , Rol Maria Angeles
Published: August 2017

The central circadian pacemaker (Suprachiasmatic Nuclei, SCN) maintains the phase relationship with the external world thanks to the light/dark cycle. Light intensity, spectra, and timing are important for SCN synchronisation. Exposure to blue-light at night leads to circadian misalignment that could be avoided by using less circadian-disruptive wavelengths. This study tests the capacity of a diurnalOctodon degusand nocturnalRattus norvegicusto synchronise to different nocturnal lights. Animals were subjected to combined red-green-blue lights (RGB) during the day and to: darkness; red light (R); combined red-green LED (RG) lights; and combined red-green-violet LED (RGV) lights during the night. Activity rhythms free-ran in rats under a RGB:RG cycle and became arrhythmic under RGB:RGV. Degus remained synchronised, despite the fact that day and night-time lighting systems differed only in spectra, but not in intensity. For degus SCN c-Fos activation by light was stronger with RGB-light than with RGV. This could be relevant for developing lighting that reduces the disruptive effects of nocturnal light in humans, without compromising chromaticity.
Shock formation and structure in magnetic reconnection with a streaming flow
Authors: Wu Liangneng , Ma Zhiwei , Zhang Haowei
Published: August 2017

The features of magnetic reconnection with a streaming flow have been investigated on the basis of compressible resistive magnetohydrodynamic (MHD) model. The super-Alfvenic streaming flow largely enhances magnetic reconnection. The maximum reconnection rate is almost four times larger with super-Alfvenic streaming flow than sub-Alfvénic streaming flow. In the nonlinear stage, it is found that there is a pair of shocks observed in the inflow region, which are manifested to be slow shocks for sub-Alfvénic streaming flow, and fast shocks for super-Alfvénic streaming flow. The quasi-period oscillation of reconnection rates in the decaying phase for super-Alfvénic streaming flow is resulted from the different drifting velocities of the shock and the X point.
Real time monitoring of water level and temperature in storage fuel pools through optical fibre sensors
Authors: Rizzolo S. , Périsse J. , Boukenter A. , Ouerdane Y. , Marin E. , Macé J-R. , Cannas M. , Girard S.
Published: August 2017

We present an innovative architecture of a Rayleigh-based optical fibre sensor for the monitoring of water level and temperature inside storage nuclear fuel pools. This sensor, able to withstand the harsh constraints encountered under accidental conditions such as those pointed-out during the Fukushima-Daiichi event (temperature up to 100 °C and radiation dose level up to ~20 kGy), exploits the Optical Frequency Domain Reflectometry technique to remotely monitor a radiation resistant silica-based optical fibre i.e. its sensing probe. We validate the efficiency and the robustness of water level measurements, which are extrapolated from the temperature profile along the fibre length, in a dedicated test bench allowing the simulation of the environmental operating and accidental conditions. The conceived prototype ensures an easy, practical and no invasive integration into existing nuclear facilities. The obtained results represent a significant breakthrough and comfort the ability of the developed system to overcome both operating and accidental constraints providing the distributed profiles of the water level (0–to–5 m) and temperature (20–to–100 °C) with a resolution that in accidental condition is better than 3 cm and of ~0.5 °C respectively. These new sensors will be able, as safeguards, to contribute and reinforce the safety in existing and future nuclear power plants.
Identification of candidate genes for devil facial tumour disease tumourigenesis
Authors: Taylor Robyn L. , Zhang Yiru , Schöning Jennifer P. , Deakin Janine E.
Published: August 2017

Devil facial tumour (DFT) disease, a transmissible cancer where the infectious agent is the tumour itself, has caused a dramatic decrease in Tasmanian devil numbers in the wild. The purpose of this study was to take a candidate gene/pathway approach to identify potentially perturbed genes or pathways in DFT. A fusion of chromosome 1 and X is posited as the initial event leading to the development of DFT, with the rearranged chromosome 1 material now stably maintained as the tumour spreads through the population. This hypothesis makes chromosome 1 a prime chromosome on which to search for mutations involved in tumourigenesis. As DFT1 has a Schwann cell origin, we selected genes commonly implicated in tumour pathways in human nerve cancers, or cancers more generally, to determine whether they were rearranged in DFT1, and mapped them using molecular cytogenetics. Many cancer-related genes were rearranged, such as the region containing the tumour suppressor NF2 and a copy gain for ERBB3, a member of the epidermal growth factor receptor family of receptor tyrosine kinases implicated in proliferation and invasion of tumours in humans. Our mapping results have provided strong candidates not previously detected by sequencing DFT1 genomes.
A patterned single layer graphene resistance temperature sensor
Authors: Davaji Benyamin , Cho Hak Dong , Malakoutian Mohamadali , Lee Jong-Kwon , Panin Gennady , Kang Tae Won , Lee Chung Hoon
Published: August 2017

Micro-fabricated single-layer graphenes (SLGs) on a silicon dioxide (SiO2)/Si substrate, a silicon nitride (SiN) membrane, and a suspended architecture are presented for their use as temperature sensors. These graphene temperature sensors act as resistance temperature detectors, showing a quadratic dependence of resistance on the temperature in a range between 283 K and 303 K. The observed resistance change of the graphene temperature sensors are explained by the temperature dependent electron mobility relationship (~T−4) and electron-phonon scattering. By analyzing the transient response of the SLG temperature sensors on different substrates, it is found that the graphene sensor on the SiN membrane shows the highest sensitivity due to low thermal mass, while the sensor on SiO2/Si reveals the lowest one. Also, the graphene on the SiN membrane reveals not only the fastest response, but also better mechanical stability compared to the suspended graphene sensor. Therefore, the presented results show that the temperature sensors based on SLG with an extremely low thermal mass can be used in various applications requiring high sensitivity and fast operation.
Activation of an anti-bacterial toxin by the biosynthetic enzyme CysK: mechanism of binding, interaction specificity and competition with cysteine synthase
Authors: Benoni Roberto , Beck Christina M. , Garza-Sánchez Fernando , Bettati Stefano , Mozzarelli Andrea , Hayes Christopher S. , Campanini Barbara
Published: August 2017

Contact-dependent growth inhibition (CDI) is a wide-spread mechanism of inter-bacterial competition. CDI+bacteria deliver CdiA-CT toxins into neighboring bacteria and produce specific immunity proteins that protect against self-intoxication. The CdiA-CT toxin from uropathogenicEscherichia coli536 is a latent tRNase that is only active when bound to the cysteine biosynthetic enzyme CysK. Remarkably, the CysK:CdiA-CT binding interaction mimics the ‘cysteine synthase’ complex of CysK:CysE. The C-terminal tails of CysE and CdiA-CT each insert into the CysK active-site cleft to anchor the respective complexes. The dissociation constant for CysK:CdiA-CT (Kd~ 11 nM) is comparable to that of theE. colicysteine synthase complex (Kd~ 6 nM), and both complexes bind through a two-step mechanism with a slow isomerization phase after the initial encounter. However, the second-order rate constant for CysK:CdiA-CT binding is two orders of magnitude slower than that of the cysteine synthase complex, suggesting that CysE should outcompete the toxin for CysK occupancy. However, we find that CdiA-CT can effectively displace CysE from pre-formed cysteine synthase complexes, enabling toxin activation even in the presence of excess competing CysE. This adventitious binding, coupled with the very slow rate of CysK:CdiA-CT dissociation, ensures robust nuclease activity in target bacteria.
A Clean and Facile Synthesis Strategy of MoS2 Nanosheets Grown on Multi-Wall CNTs for Enhanced Hydrogen Evolution Reaction Performance
Authors: Cao Jiamu , Zhou Jing , Zhang Yufeng , Liu Xiaowei
Published: August 2017

Unique hybrid nanostructure, which consists of multi-wall carbon nanotube (MWCNT) stems and MoS2 nanosheet (NS) leaves, are prepared by a hydrothermal method. The fabricated material can be potentially used as an electrocatalyst for the hydrogen evolution reaction (HER). To our knowledge, as the reaction medium, water is firstly utilized to the synthesis of the 1T phase MoS2 NSs which uniformly grow on the carbon-based materials. As a result, a nanohybrid catalyst with excellent HER electrocatalytic properties, which included an onset potential of as low as 50 mV, a Tafel slope of 43 mV dec−1, and remarkable cycling stability, is produced. The observed outstanding catalytic performance can be attributed to the uniform distribution of the metallic 1T phase of the MoS2 NSs, which are characterized by the presence of multiple active edges as well as the effective electron transport route provided by the conductive MWCNT substrate. This work demonstrates the high potential of the synthesized HER catalyst and proposes a novel, efficient, environmentally friendly, and inexpensive method for its fabrication.
Determination of Phenols Isomers in Water by Novel Nanosilica/Polydimethylsiloxane-Coated Stirring Bar Combined with High Performance Liquid Chromatography-Fourier Transform Infrared Spectroscopy
Authors: Zheng Bei , Li Wentao , Liu Lin , Wang Xin , Chen Chen , Yu Zhiyong , Li Hongyan
Published: August 2017

A novel nanosilica/polydimethylsiloxane (SiO2/PDMS) coated stirring bar was adopted in the sorption extraction (SBSE) of phenols in water, and the high performance liquid chromatography-fourier transform infrared spectroscopy (HPLC-FTIR) was subsequently used to determination of phenol concentration. The SiO2/PDMS coating was prepared by sol-gel method and characterized with respect to morphology and specific surface area. The results of field-emission scanning electron microscope (FE-SEM) and N2 adsorption-desorption as well as phenol adsorption experiments denoted that SiO2/PDMS has larger surface area and better adsorption capacity than commercial PDMS. The extraction efficiency of phenol with SiO2/PDMS coated stirring bar was optimized in terms of ion strength, flow rate of phenol-involved influent, type of desorption solvent and desorption time. More than 75% of phenol desorption efficiency could be kept even after 50 cycles of extraction, indicating the high stability of the SiO2/PDMS coated stirring bar. Approximately 0.16 mg/L 2, 5-dimethylphenol (2, 5-DMP), which was 34-fold more toxic than phenol, was detected in water through HPLC-FTIR. However, 2, 5-DMP could be oxidized to 5-methy-2-hydroxy benzaldehyde after disinfection in drinking water treatment process. Therefore, the proposed method of SiO2/PDMS-SBSE-HPLC-FTIR is successfully applied in the analysis of phenols isomers in aqueous environment.
An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
Authors: Kim Hye-Ran , Lee Hyun-Su , Lee Kyung-Sik , Jung In Duk , Kwon Min-Sung , Kim Chang-Hyun , Kim Seong-Min , Yoon Myung-Han , Park Yeong-Min , Lee Sang-Myeong , Jun Chang-Duk
Published: August 2017

Activated macrophages have a greater ability of phagocytosis against pathogens that is mediated by large-scale actin rearrangement. However, molecular machineries that conduct this task have not been fully identified. Here, we demonstrate an unanticipated role of TAGLN2, a 22-kDa actin-binding protein, in Toll-like receptor (TLR)-stimulated phagocytosis. TAGLN2 was greatly induced in macrophages in response to lipopolysaccharide (LPS), a ligand for TLR4, partly via the NF-κB pathway. TAGLN2-deficient macrophages (TAGLN2−/−) showed defective phagocytic functions of IgM- and IgG-coated sheep red blood cells as well as bacteria. Cell signaling pathways involved in actin rearrangement—PI3 kinase/AKT and Ras-ERK—were also down-regulated in LPS-stimulated TAGLN2-deficient macrophages. Moreover,TAGLN2−/−mice showed higher mortality after bacterial infection than wild-type littermates. Thus, our results revealed a novel function of TAGLN2 as a molecular armament required for host defense.