bluuebunny/crossref_metadata_2025 huggingface dataset:
Path Planning of Robot Based on Improved Multi-Strategy Fusion Whale Algorithm
Authors: You Dazhang , Kang Suo , Yu Junjie , Wen Changjun
Published: August 2024

In logistics and manufacturing, smart technologies are increasingly used, and warehouse logistics robots (WLR) have thus become key automation tools. Nonetheless, the path planning of mobile robots in complex environments still faces the challenges of excessively long paths and high energy consumption. To this end, this study proposes an innovative optimization algorithm, IWOA-WLR, which aims to optimize path planning and improve the shortest route and smoothness of paths. The algorithm is based on the Whale Algorithm with Multiple Strategies Fusion (IWOA), which significantly improves the obstacle avoidance ability and path optimization of mobile robots in global path planning. First, improved Tent chaotic mapping and differential dynamic weights are used to enhance the algorithm’s optimization-seeking ability and improve the diversity of the population. In the late stage of the optimization search, the positive cosine inertia threshold and the golden sine are used to perform adaptive position updating during the search strategy to enhance the global optimal search capability. Secondly, the fitness function of the path planning problem is designed, and the path length is taken as the objective function, the path smoothness as the evaluation index, and the multi-objective optimization is realized through the hierarchical adjustment strategy and is applied to the global path planning of WLR. Finally, simulation experiments on raster maps with grid sizes of 15 × 15 and 20 × 20 compare the IWOA algorithm with the WOA, GWO, MAACO, RRT, and A* algorithms. On the 15 × 15 maps, the IWOA algorithm reduces path lengths by 3.61%, 5.90%, 1.27%, 15.79%, and 5.26%, respectively. On the 20 × 20 maps, the reductions are 4.56%, 5.83%, 3.95%, 19.57%, and 1.59%, respectively. These results indicate that the improved algorithm efficiently and reliably finds the global optimal path, significantly reduces path length, and enhances the smoothness and stability of the path’s inflection points.
Projections for the 2050 Scenario of the Mexican Electrical System
Authors: Toledo-Vázquez Diocelina , Romero Rosenberg J. , Hernández-Luna Gabriela , Cerezo Jesús , Montiel-González Moisés
Published: August 2024

Electricity is fundamental to modern societies and will become even more so as its use expands through different technologies and population growth. Power generation is currently the largest source of carbon-dioxide (CO2) emissions globally, but it is also the sector that is leading the transition to net zero emissions through the rapid rise of renewables. The impacts of COVID-19 on the electricity sector led to a reduction in the demand for electricity, while at the same time, the current global energy crisis has placed the security and affordability of electricity at the top of the political agenda in many countries. In this way, the decrease in the demand for electricity, as well as its gradual recovery, makes it necessary to carry out energy planning that considers the adverse effects caused by global events with a high socioeconomic impact. In this article, the Low Emission Analysis Platform (LEAP) 2020 software has been used to determine the distribution of energy sources to 2050 for Mexico. The variables that lead to the possible profiles for 2050 are social, economic, and technological. The results correspond to a possible future based on official data from the National Electric System (SEN) of Mexico. The forecast for 2050 indicates that the electricity sector will have almost double the current installed capacity; however, emissions do not correspond to twice as much: they are practically 50% higher.
Suitability and Structural Optimization of Vegetation Restoration on the Loess Plateau: A MaxEnt Model-Based Study of Environmental and Anthropogenic Impacts
Authors: Luo Jie , Chen Yirui , Wu Ying , Xie Guoying , Jia Weitian , Sardar Muhammad Fahad , Binobead Manal Abdulaziz , Li Xiang
Published: August 2024

In recent years, the problem of ecosystem degradation caused by human activities has become increasingly serious. Vegetation restoration is a key means to solve this problem, which has increased. To address the suitability and structural optimization of vegetation restoration in the Loess Plateau (China), the MaxEnt model was used to quantify the impacts of environmental and human activities on the planting suitability of vegetation restoration species at the raster scale. Three layers of trees, shrubs, and herbs with 12 common vegetation restoration species were selected. The factor index system was constructed by combining climatic, ecological, and socio-economic data, and the MaxEnt model predicted land suitability. It was found that human activities significantly increased the unsuitable planting area. This especially affected Robinia pseudoacacia in the tree layer and Amorpha fruticosa in the shrub layer. High and medium suitable areas were mainly sparsely populated areas with close water sources. Through maximum suitability optimization, it was identified that the overall spatial distribution of the three layers in the study area was relatively consistent, and the structural dominance of trees + shrubs + herbs and single herbs in the vertical structure was obvious; these were concentrated in the southwestern and northeastern parts of the study area, respectively. In addition, organic content (OC) and distance from the road to woodland (RW) were the dominant factors affecting land suitability, with a contribution rate of more than 50% and up to 80%. These results provide a scientific basis for optimizing planting structures. They are of significant theoretical and practical significance in guiding vegetation restoration work.
Effects of Different Types of Starch on Physicochemical Properties and Microstructure of Beef during Cold Storage
Authors: Zhang Shulin , Wang Lina , Wang Qiuyu , Wang Yuqi , Wang Linlin , Du Rongsheng
Published: August 2024

The purpose of this study was to identify the most effective method for enhancing the quality of beef gel during refrigeration. To achieve this objective, the effects of various types of starch on the physicochemical properties and microstructure of beef gel during refrigeration were investigated. In this study, ground beef gel was chosen as the research subject, and six different types of starch were added: 6% tapioca starch, cassava-modified starch (acetylated distarch phosphate, ADSP), potato starch (PSP), modified potato starch (acetate starch, SA), corn starch (CSP), and modified corn starch (hydroxypropyl distarch phosphate, HPDSP). The quality indicators of ground beef were measured and analyzed throughout the cold storage at 4 °C on days 1, 3, 5, 7, and 9. The results demonstrated that the water capacity of beef mince supplemented with PSP and HPDSP was significantly greater (p < 0.05). Additionally, the gel strength was found to be the highest, while the mesh structure formed in the ADSP group was the greatest. Furthermore, HPDSP, PSP, and SA effectively inhibited the oxidation of meat fat, with SA showing a relatively good effect on delaying the oxidation of meat mince protein. The addition of starch can, to a certain extent, inhibit lipid and protein oxidation in meat mince. In conclusion, starch significantly enhances the quality of beef mince by improving water retention, gel strength, and microstructure during refrigeration.
Garlic Extract Increased Acrylamide Formation in French Fries Obtained by Different Cooking Methods
Authors: Sipahi Simge , Barak Timur Hakan , Can Özge , Temur Betül Zehra , Baş Murat , Sağlam Duygu
Published: August 2024

Fried potato products are the largest dietary source of acrylamide, a potential carcinogen formed at high temperatures. Previous studies suggested that garlic powder could decrease the development of acrylamide; however, there has not been much focus on the effect of garlic extract. The aim of this study was to investigate the effect of garlic extract exposure on the development of acrylamide in French fries in popular home cooking techniques such as pan-frying, air-frying, and oven-frying. Initially, the antioxidative profile, total phenolic content, and chlorogenic acid content of garlic were analyzed. Subsequently, potatoes were treated with garlic extract and fried using pan-frying, air-frying, and oven-frying techniques. Acrylamide levels were then quantified through HPLC and compared to control groups. The findings showed that garlic exposure increased the acrylamide formation in French fries obtained with air-frying (311.95 ± 0.5 μg/kg) and with oven-frying (270.32 ± 23.4 μg/kg) (p < 0.005 *). This study offers new insights into varying acrylamide formation levels in domestic practices. Unlike previous studies, this study is the first to question the effect of aqueous garlic extract exposure. Further research is required to comprehend the interaction between garlic exposure and acrylamide formation in household settings.
Effects of Different Roasting Methods on the Quality of Roasted Large Yellow Croaker (Larimichthys crocea)
Authors: Yin Chenjing , Zhang Chao , Xu Yangli , Su Laijin
Published: August 2024

This study investigated the effects of different roasting methods (45% light wave and 55% microwave roasting, 70% light wave and 30% microwave roasting, 100% light wave roasting, far-infrared roasting, and oven roasting) on the quality of roasted large yellow croaker. The quality was evaluated using sensory evaluation, texture characteristics, color differences, moisture content, and volatile flavor substances. In this context, different roasting methods can affect the color, taste, and flavor of large yellow croaker fish, significantly improving the overall acceptance of roasted fish. The results showed that after 45% light wave and 55% microwave roasting, the elasticity of fish meat was maintained, the hardness of fish meat was reduced, the moisture content and distribution were changed, and the taste was the best. Far-infrared roasting and 45% light wave and 55% microwave roasting had a significant effect on the color of large yellow croaker samples and improved the sensory evaluation score. Forty-six volatile compounds were detected using gas chromatography–mass spectrometry. After roasting, the oxidation and Maillard reactions of lipids and proteins were increased, with the 45% light wave and 55% microwave roasting giving the highest variety of volatile flavor substance products.
Basic Theory of Ice Crystallization Based on Water Molecular Structure and Ice Structure
Authors: Zheng Ouyang , Zhang Li , Sun Qinxiu , Liu Shucheng
Published: August 2024

Freezing storage is the most common method of food preservation and the formation of ice crystals during freezing has an important impact on food quality. The water molecular structure, mechanism of ice crystal formation, and ice crystal structure are elaborated in the present review. Meanwhile the methods of ice crystal characterization are outlined. It is concluded that the distribution of the water molecule cluster structure during the crystallization process directly affects the formed ice crystals’ structure, but the intrinsic relationship needs to be further investigated. The morphology and distribution of ice crystals can be observed by experimental methods while simulation methods provide the possibility to study the molecular structure changes in water and ice crystals. It is hoped that this review will provide more information about ice crystallization and promote the control of ice crystals in frozen foods.
Consumer Preference and Willingness to Pay for Rice Attributes in China: Results of a Choice Experiment
Authors: Fang Pingping , Zhou Zhou , Wang Hua , Zhang Lixia
Published: August 2024

Understanding urban consumers’ preferences for rice attributes is crucial for rice breeders, producers, and retailers to meet diverse and evolving market demands. Based on the sample data of 629 rice consumers in Shanghai, China, obtained through the choice experiment (CE) approach, this study uses the mixed logit (ML) model to analyze consumers’ preferences and willingness to pay (WTP) for food safety labels, brands, nutritional quality, and taste quality. Furthermore, the latent class (LC) model examines the heterogeneity in consumer group preferences. The research findings highlight that consumers prioritize taste quality as the most crucial attribute, followed by nutritional quality, food safety labels, and brand attributes. The premium rates for superior taste quality, organic certification labels, and green certification labels exceeded 100%. Interestingly, while combining organic certification with well-known international or domestic brands does not uniformly boost consumer preferences, incorporating green certification alongside well-known international or domestic brands significantly elevates those preference levels. Factors such as the external environment, consumption habits, and personal characteristics significantly influence individuals’ preferences for rice attributes. Based on these insights, the study puts forth policy recommendations for rice breeders, producers, and retailers.
Lessons Learned and Outcomes from Risk-Based Modernisation of Post-Mortem Inspection and Disposition Criteria of Beef, Sheep, Goat, and Pig Carcasses in Australia
Authors: Pointon Andrew , Kiermeier Andreas , Hamilton David , Allan Samantha , Jenson Ian , Stevens Daryl , McDonald Ann , Langbridge John
Published: August 2024

The lessons learned from reviewing national risk assessments to modernise the Australian Standard for the post-mortem inspection and disposition judgement of beef, sheep, goat, and pig carcases are discussed. The initial risk profiles identified priorities for quantitative assessments. Broadly, the main difficulty encountered was the paucity of quantified performance for the current inspection. Resolving this involved acquiring gross abnormality data representing regional production/proportional abattoir volumes, the range of gross abnormalities appearing nationally, proportional occurrence at carcase sites, and seasonality to enable the comparison of procedures. The methodologies followed the Codex Alimentarius Commission’s risk assessment guidelines and are fully documented in the associated publications. The evidence and discussion are provided for the associated challenges experienced, including preventing contamination, the use of food chain information to support amendment, inspection as a part of industry Quality Assurance programmes, and opportunities to improve inspector training. The criteria considered by the Competent Authority for the determination of the equivalence of alternative post-mortem inspection techniques included comparisons of public health risk, non-detection rates for gross abnormalities, and microbial contamination resulting from inspection activities, as appropriate. Most of the gross abnormalities detected arose from animal health and welfare conditions affecting wholesomeness and did not present as food safety hazards. The non-detection rates between the current and alternative inspection (observation) were negligible. A quantitative risk assessment for Cysticercus bovis was conducted. Carcases with multiple gross abnormalities predominantly reflected historic infections (prior septicaemia), where trimming achieved wholesomeness unless they were cachexic.
A Method for Sorting High-Quality Fresh Sichuan Pepper Based on a Multi-Domain Multi-Scale Feature Fusion Algorithm
Authors: Xiang Pengjun , Pan Fei , Duan Xuliang , Yang Daizhuang , Hu Mengdie , He Dawei , Zhao Xiaoyu , Huang Fang
Published: August 2024

Post-harvest selection of high-quality Sichuan pepper is a critical step in the production process. To achieve this, a visual system needs to analyze Sichuan pepper with varying postures and maturity levels. To quickly and accurately sort high-quality fresh Sichuan pepper, this study proposes a multi-scale frequency domain feature fusion module (MSF3M) and a multi-scale dual-domain feature fusion module (MS-DFFM) to construct a multi-scale, multi-domain fusion algorithm for feature fusion of Sichuan pepper images. The MultiDomain YOLOv8 Model network is then built to segment and classify the target Sichuan pepper, distinguishing the maturity level of individual Sichuan peppercorns. A selection method based on the average local pixel value difference is proposed for sorting high-quality fresh Sichuan pepper. Experimental results show that the MultiDomain YOLOv8-seg achieves an mAP50 of 88.8% for the segmentation of fresh Sichuan pepper, with a model size of only 5.84 MB. The MultiDomain YOLOv8-cls excels in Sichuan pepper maturity classification, with an accuracy of 98.34%. Compared to the YOLOv8 baseline model, the MultiDomain YOLOv8 model offers higher accuracy and a more lightweight structure, making it highly effective in reducing misjudgments and enhancing post-harvest processing efficiency in agricultural applications, ultimately increasing producer profits.
Exploring the Volatile Profile of Vanilla planifolia after Fermentation at Low Temperature with Bacillus Isolates
Authors: Manyatsi Thabani-Sydney , Lin Yu-Hsin , Sung Pin-Hui , Jou Ying-Tzy
Published: August 2024

Vanilla planifolia is grown as a high-value orchid spice for its odor and savor attributes that increase due to the curing process associated with microbial colonization. This tends to influence the aromatic properties of vanilla. Hence, 11 Bacillus sp. strains were isolated from V. planifolia and identified with 16S rRNA gene sequencing. The liquid culture (1 mL of 107 CFU mL−1) of selected Bacillus vallismortis NR_104873.1:11-1518, Bacillus velezensis ZN-S10, and Bacillus tropicus KhEp-2 effectively fermented green-blanched vanilla pods kept at 10 °C during the sweating stage. GC-MS analysis showed that the methanol extract of non-coated, and B. vallismortis treated vanilla detected three (3) volatile compounds, whereas seven (7) components were obtained in B. tropicus and B. velezensis treatment. 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl was found in B. velezensis ZN-S10, B. tropicus KhEp-2, and B. vallismortis while it was not present in the control samples. This ketone compound suggested a Maillard reaction resulting in brown-increased aroma pods. Linoleic acid and Hexadecanoic acid ethyl esters were detected only in ZN-S10 strain-coated vanilla. A novel 3-Deoxy-d-mannoic lactone was detected only in B. vallismortis-treated vanilla characterized as a new compound in V. planifolia which suggested that the new compound can be altered with the coating of bacteria in vanilla during fermentation. Thus, the Bacillus strains improved the volatile profile and exhibited a new aroma and flavor profile of vanilla owing to bacteria fermentation during the curing process.
Monotonic Drained and Undrained Shear Behaviors of Compacted Slightly Weathered Tephras from New Zealand
Authors: Sood Shaurya , Chiaro Gabriele , Wilson Thomas , Stringer Mark
Published: August 2024

This paper reports and discusses the results of a series of monotonic compression drained and undrained triaxial tests performed on three compacted, slightly weathered silty sand tephras. In total, 18 drained and 18 undrained tests were performed on compacted specimens (at Dc ≈ 90 and 100%) isotropically consolidated at confining pressures of 50–200 kPa. It was observed that particle size distribution, weathering state, and mineralogy of the tephra deposits had significant effects on the stress–strain responses, friction angles, stress–dilatancy relations, and critical state characteristics. For instance, the coarser tephra (namely white–grey Kaharoa, that was less affected by weathering processes) showed a primarily dilative response. The effects of chemical composition, namely weathering degree and mineralogy, on geotechnical properties such as friction angle were investigated with an attempt to interlink the two characteristics for heterogeneous tephras. The measured friction angles (ϕ = 32.7°–42.8°), combined with the results of weathering degrees and mineralogical investigations, indicated that silty sand tephras, if properly compacted, are suitable fills for use in typical geotechnical applications.
Psychometric Evaluation of the Brief-COPE Inventory and Exploration of Factors Associated with Perceived Stress among Peruvian Nurses
Authors: Zeladita-Huaman Jhon Alex , Flores-Rodríguez Carmen Cristina , Zegarra-Chapoñan Roberto , Carpio-Borja Sugely Julia , Franco-Chalco Eduardo , Vivas-Durand Teresa De Jesús , Castillo-Parra Henry , Alvarado-Rivadeneyra Silas Hildeliza , Mariñas-Acevedo Orfelina
Published: August 2024

Background: This study aimed to analyze the psychometric properties of the Brief-COPE Inventory and to determine its concurrent validity by examining its association with perceived stress among Peruvian nurses. Methods: A psychometric study was conducted with 434 Peruvian nurses to evaluate the psychometric properties of the Brief-COPE Inventory through confirmatory factor analysis. Three stepwise variable selection regression models were implemented. Results: The three-factor model of the Brief-COPE Inventory demonstrated adequate fit indices (root mean square error of approximation = 0.052, standardized root mean square residual = 0.068, and both the comparative fit index and the Tucker–Lewis index = 0.95). Additionally, the factors were significantly correlated (p < 0.001), and the reliability was adequate (ω = 0.90). Nurses reported a medium level of perceived stress, with associated factors including having received stress management training, fear of COVID-19, and problem-focused coping strategies (p < 0.05). Conclusion: This study confirms that the Brief-COPE Inventory is a valid tool for measuring coping strategies among Peruvian nurses due to its good model fit, excellent reliability, and concurrent validity with perceived stress. However, further research is needed to assess its validity in the specific areas of performance perceived by nursing professionals.
Hyperbaric Oxygen Therapy during Pregnancy for Critical Anemia Secondary to Sickle Cell Disease with Post-Transfusion Hyperhemolysis: A Case Report
Authors: Khan Shawn , Brenna Connor T. A. , Pendergrast Jacob , Malinowski A. Kinga , Salvatori Marcus , Katznelson Rita , Tarshis Jordan
Published: August 2024

Background: Sickle cell disease is the most common human monogenetic disease, and its risks are amplified during pregnancy. Methods: This report describes a 35-year-old woman with HgbSS sickle cell disease who developed hyperhemolysis syndrome after undergoing an exchange transfusion during pregnancy. Results: In addition to conventional medical treatment, the patient received prepartum hyperbaric oxygen therapy (HBOT), totaling 17 treatments for the indication of severe anemia. She experienced significant clinical improvement while undergoing HBOT and ultimately delivered a healthy preterm infant by cesarean section. Conclusions: The risks, benefits, and challenges of using HBOT in this unique context are discussed.
Effects of Apical, Late-Season Leaf Removal on Vine Performance and Wine Properties in Sangiovese Grapevines (Vitis vinifera L.)
Authors: Vercesi Alberto , Gabrielli Mario , Garavani Alessandra , Poni Stefano
Published: August 2024

An urgent challenge posed by climate change in warm grapevine-growing areas is accelerated ripening, which leads to rapid sugar accumulation while phenolics and aroma traits lag behind. Techniques that enable selectively delaying the sugar accumulation process without affecting the accumulation of secondary metabolites are essential. This study aimed to evaluate the effects of apical-to-cluster defoliation, manually applied in 2019 at the onset of veraison (D1) or 20 days later (D2), which removed about 30–40% of the pending total leaf area without altering the cluster microclimate compared with a non-defoliated control (C). Ripening trends, vegetative growth, yield components, and the final grape and wine composition, as well as wine sensorial attributes, were assessed. Although both treatments significantly lowered the final leaf area-to-yield ratio (0.80–0.90 m2/kg) compared with the 1.35 m2/kg recorded in the C vines, only D1 reduced the final total soluble solids (TSS) at harvest (2 °Brix less than C). However, the total anthocyanins were similarly limited, and titratable acidity (TA) did not differ from the C vines. The D1 wine was deemed similar to that made from control plants. Conversely, D2 failed to delay ripening, yet the D2 wine was deemed superior in terms of olfactory intensity, body, fruitiness, balance, and overall preference. Although the study was conducted over a single season, the results are robust enough to conclude that the timing of defoliation—i.e., the level of TSS concurrently reached by the C treatment—is crucial to achieving specific effects. Early defoliation appears valid for postponing ripening into a cooler period, making it quite interesting in warm–hot areas with a very long growing season; a much later defoliation, likely due to the interaction between mean canopy age and more light filtering from above the cluster zone, can elevate the quality of and appreciation for the final wine.
QuickPic AAC: An AI-Based Application to Enable Just-in-Time Generation of Topic-Specific Displays for Persons Who Are Minimally Speaking
Authors: Yu Christina , Schlosser Ralf W. , Fontana de Vargas Maurício , White Leigh Anne , Koul Rajinder , Shane Howard C.
Published: August 2024

As artificial intelligence (AI) makes significant headway in various arenas, the field of speech–language pathology is at the precipice of experiencing a transformative shift towards automation. This study introduces QuickPic AAC, an AI-driven application designed to generate topic-specific displays from photographs in a “just-in-time” manner. Using QuickPic AAC, this study aimed to (a) determine which of two AI algorithms (NLG-AAC and GPT-3.5) results in greater specificity of vocabulary (i.e., percentage of vocabulary kept/deleted by clinician relative to vocabulary generated by QuickPic AAC; percentage of vocabulary modified); and to (b) evaluate perceived usability of QuickPic AAC among practicing speech–language pathologists. Results revealed that the GPT-3.5 algorithm consistently resulted in greater specificity of vocabulary and that speech–language pathologists expressed high user satisfaction for the QuickPic AAC application. These results support continued study of the implementation of QuickPic AAC in clinical practice and demonstrate the possibility of utilizing topic-specific displays as just-in-time supports.
A Collaborative Approach to Understanding the Intersections of Practice and Policy for Peers in the Alcohol and Other Drugs Sector
Authors: Piatkowski Timothy , Kill Emma
Published: August 2024

Introduction: Peers in the alcohol and other drug sectors possess lived–living experience (LLE) crucial for shaping community care. However, genuine consumer collaboration is often confounded by stigma. This study examined peers’ perceptions, exploring their experiences regarding workforce dynamics, policy implications, and impacts on health equity. In presenting the research, we sought to synthesise the research methods and illustrate the methodological innovation and knowledge production in substance use research through authentic collaboration. Methods: We purposively sampled peer networks and community organisations, involving peer-researchers in planning, design, and analysis. We conducted semi-structured digital interviews with 18 peers and applied iterative coding to analyse the data. Results: This collaborative process provided nuanced insights into sectoral challenges. Peers expressed emotional strain revisiting personal substance use experiences, blurring personal and professional boundaries. Tokenistic peer involvement critiques underscored the need for genuine leadership and organisational support. Conclusion: We advocate for a shift towards equitable and inclusive policy development through both organisational and systemic restructuring. However, these changes are hamstrung by broader policy frameworks, which require a shift to peer-led principles, ensuring the expertise of peers is genuinely valued. Policymakers should invest in expanding peer frameworks, acknowledging the diversity within communities of people who use drugs to improve health equity and public health outcomes. This innovative approach to substance use research emphasises the transformative impact of integrating LLE into research.
Significance of Fibrillin-1, Filamin A, MMP2 and SOX9 in Mitral Valve Pathology
Authors: Opris Carmen Elena , Suciu Horatiu , Jung Ioan , Flamand Sanziana , Harpa Marius Mihai , Opris Cosmin Ioan , Popa Cristian , Kovacs Zsolt , Gurzu Simona
Published: August 2024

Genetic factors play a significant role in the pathogenesis of mitral valve diseases, including mitral valve prolapse (MVP) and mitral valve regurgitation. Genes like Fibrillin-1 (FBN1), Filamin A (FLNA), matrix metalloproteinase 2 (MMP2), and SRY-box transcription factor 9 (SOX9) are known to influence mitral valve pathology but knowledge of the exact mechanism is far from clear. Data regarding serum parameters, transesophageal echocardiography, and genetic and histopathologic parameters were investigated in 54 patients who underwent cardiovascular surgery for mitral valve regurgitation. The possible association between Fibrillin-1, Filamin A, MMP2, and SOX9 gene expressions was checked in relationship with the parameters of systemic inflammatory response. The mRNA expression levels (RQ—relative quantification) were categorized into three distinct groups: low (RQ < 1), medium/normal (RQ = 1–2), and high (RQ > 2). Severe fibrosis of the mitral valve was reflected by high expression of FBN1 and low expression of MMP2 (p < 0.05). The myxoid degeneration level was associated with the mRNA expression level for FBN1 and a low lymphocyte-monocyte ratio was associated with an increased mRNA expression of FBN1 (p < 0.05). A high number of monocytes was associated with high values of FBN1 whereas the increase in the number of lymphocytes was associated with high levels of MMP2. In addition, we observed that the risk of severe hyalinization was enhanced by a low mRNA expression of FLNA and/or SOX9. In conclusion, a lower FLNA mRNA expression can reflect the aging process that is highlighted in mitral valve pathology as a higher risk for hyalinization, especially in males, that might be prevented by upregulation of the SOX9 gene. FBN1 and MMP2 influence the inflammation-related fibrotic degeneration of the mitral valve. Understanding the genetic base of mitral valve pathology can provide insights into disease mechanisms, risk stratification, and potential therapeutic targets.
Expression of MicroRNAs in Adults with Celiac Disease: A Narrative Review
Authors: Rigo Francielen Furieri , Oliveira Ellen Cristina Souza de , Quaglio Ana Elisa Valencise , Moutinho Bruna Damásio , Di Stasi Luiz Claudio , Sassaki Ligia Yukie
Published: August 2024

Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of proline- and glutamine-rich proteins, widely termed “gluten”, in genetically susceptible individuals. CD induces an altered immune response that leads to chronic inflammation and duodenal mucosal damage. Currently, there are no specific tests for the accurate diagnosis of CD, and no drugs are available to treat this condition. The only available treatment strategy is lifelong adherence to a gluten-free diet. However, some studies have investigated the involvement of microRNAs (miRNAs) in CD pathogenesis. miRNAs are small noncoding ribonucleic acid molecules that regulate gene expression. Despite the growing number of studies on the role of miRNAs in autoimmune disorders, data on miRNAs and CD are scarce. Therefore, this study aimed to perform a literature review to summarize CD, miRNAs, and the potential interactions between miRNAs and CD in adults. This review shows that miRNA expression can suppress or stimulate pathways related to CD pathogenesis by regulating cell proliferation and differentiation, regulatory T-cell development, innate immune response, activation of the inflammatory cascade, focal adhesion, T-cell commitment, tissue transglutaminase synthesis, and cell cycle. Thus, identifying miRNAs and their related effects on CD could open new possibilities for diagnosis, prognosis, and follow-up of biomarkers.
The Role of Serum Albumin and Secretory Phospholipase A2 in Sepsis
Authors: Tsao Francis H. C. , Li Zhanhai , Amessoudji Amy W. , Jawdat Dunia , Sadat Musharaf , Arabi Yaseen , Meyer Keith C.
Published: August 2024

Sepsis is caused by a dysregulated host response to an infection that leads to cascading cell death and eventually organ failure. In this study, the role of inflammatory response serum secretory phospholipase A2 (sPLA2) and albumin in sepsis was investigated by determining the activities of the two proteins in serial serum samples collected on different days from patients with sepsis after enrollment in the permissive underfeeding versus standard enteral feeding protocols in an intensive care unit. Serum sPLA2 and albumin showed an inverse relationship with increasing sPLA2 activity and decreasing albumin membrane-binding activity in patients with evolving complications of sepsis. The activities of sPLA2 and albumin returned to normal values more rapidly in the permissive underfeeding group than in the standard enteral feeding group. The inverse sPLA2–albumin activity relationship suggests a complex interplay between these two proteins and a regulatory mechanism underlying cell membrane phospholipid homeostasis in sepsis. The decreased albumin–membrane binding activity in patients’ serum was due to its fatty acid-binding sites occupied by pre-bound fatty acids that might alter albumin’s structure, binding capacities, and essential functions. The sPLA2–albumin dual serum assays may be useful in determining whether nutritional intervention effectively supports the more rapid recovery of appropriate immune responses in critically ill patients with sepsis.
Synthesis, Structure, and Properties of a Copper(II) Binuclear Complex Based on Trifluoromethyl Containing Bis(pyrazolyl)hydrazone
Authors: Shakirova Olga G. , Morozova Tatiana D. , Kudyakova Yulia S. , Bazhin Denis N. , Kuratieva Natalia V. , Klyushova Lyubov S. , Lavrov Alexander N. , Lavrenova Lyudmila G.
Published: August 2024

A new complex of copper(II) with methyl-5-(trifluoromethyl)pyrazol-3-yl-ketazine (H2L) was synthesized with the composition [Cu2L2]∙C2H5OH (1). Recrystallization of the sample from DMSO yielded a single crystal of the composition [Cu2L2((CH3)2SO)] (2). The coordination compounds were studied by single-crystal X-ray diffraction analysis, IR spectroscopy, and static magnetic susceptibility method. The data obtained indicate that the polydentate ligand is coordinated by both acyclic nitrogen and heterocyclic nitrogen atoms. The cytotoxic activity of the ligand and complex 1 was investigated on human cell lines MCF7 (breast adenocarcinoma), Hep2 (laryngeal carcinoma), A549 (lung carcinoma), HepG2 (hepatocellular carcinoma), and MRC5 (non-tumor lung fibroblasts). The complex was shown to have a pronounced dose-dependent cytotoxicity towards these cell lines with LC50 values in the range of 0.18–4.03 μM.
Evaluation of Chemical Profile and Biological Properties of Extracts of Different Origanum vulgare Cultivars Growing in Poland
Authors: Betlej Izabela , Żurek Natalia , Cebulak Tomasz , Kapusta Ireneusz , Balawejder Maciej , Kiełtyka-Dadasiewicz Anna , Jaworski Sławomir , Lange Agata , Kutwin Marta , Krochmal-Marczak Barbara , Kłosińska Teresa , Nasiłowska Barbara , Mierczyk Zygmunt , Borysiuk Piotr
Published: August 2024

This research studied the phenolic content compared with the antioxidant properties of various O. vulgare (Lamiaceae) cultivars grown in Poland. The research results in this paper indicate that the dominant ingredient in all oregano cultivars was rosmarinic acid, known for its strong antioxidant properties. The highest amounts of rosmarinic acid (87.16 ± 4.03 mg/g dm) were identified in the O. vulgare spp. hirtum (Link) Ietsw. Other metabolites identified in the studied extracts include luteolin O-di-glucuronide-O-di-pentoside (30.79 ± 0.38 mg/g dm in the ‘Aureum’ cultivar), 4′-O-glucopyranosyl-3′, 4′-dihydroxy benzyl-protocatechuate (19.84 ± 0.60 mg/g dm in the ‘Margerita’ cultivar), and p-coumaroyl-triacetyl-hexoside (25.44 ± 0.18 mg/g dm in the ‘Margerita’ cultivar). ‘Hot & spicy’ and ‘Margerita’ cultivars were characterized by the highest activity in eliminating OH• and O2•− radicals. Extracts from Greek oregano had the highest ability to scavenge DPPH radicals and chelate iron ions. This research has also provided new evidence that oregano has anti-migratory, cytotoxic properties and influences the viability of gastric cancer cells (the highest cytotoxicity was attributed to the ‘Hot & spicy’ cultivar, which performed the worst in antioxidant properties tests). Extracts from the tested cultivars at a concentration of 0.625% effectively inhibited the growth of S. aureus and P. aeruginosa bacteria. It seems that the oregano grown in Poland is of good quality and can be successfully grown on a large scale if the appropriate use is found.
Radiotracers for Molecular Imaging of Angiotensin-Converting Enzyme 2
Authors: Xu Wenqi , Langhans Sigrid A. , Johnson David K. , Stauff Erik , Kandula Vinay V. R. , Kecskemethy Heidi H. , Averill Lauren W. , Yue Xuyi
Published: August 2024

Angiotensin-converting enzymes (ACE) are well-known for their roles in both blood pressure regulation via the renin-angiotensin system as well as functions in fertility, immunity, hematopoiesis, and many others. The two main isoforms of ACE include ACE and ACE-2 (ACE2). Both isoforms have similar structures and mediate numerous effects on the cardiovascular system. Most remarkably, ACE2 serves as an entry receptor for SARS-CoV-2. Understanding the interaction between the virus and ACE2 is vital to combating the disease and preventing a similar pandemic in the future. Noninvasive imaging techniques such as positron emission tomography and single photon emission computed tomography could noninvasively and quantitatively assess in vivo ACE2 expression levels. ACE2-targeted imaging can be used as a valuable tool to better understand the mechanism of the infection process and the potential roles of ACE2 in homeostasis and related diseases. Together, this information can aid in the identification of potential therapeutic drugs for infectious diseases, cancer, and many ACE2-related diseases. The present review summarized the state-of-the-art radiotracers for ACE2 imaging, including their chemical design, pharmacological properties, radiochemistry, as well as preclinical and human molecular imaging findings. We also discussed the advantages and limitations of the currently developed ACE2-specific radiotracers.
Generation of Novel Tumour-Selective SEA Superantigen-Based Peptides with Improved Safety and Efficacy for Precision Cancer Immunotherapy
Authors: Bashraheel Sara S. , Al-Sulaiti Haya , Goda Sayed K.
Published: August 2024

Bacterial superantigens are T-cell-stimulatory protein molecules which produce massive cytokines and cause human diseases. Due to their ability to activate up to 20% of resting T-cells, they have effectively killed T-cell-dependent tumours in vivo. However, the intrinsic toxicity of whole SAg molecules highlights the urgent need to develop more effective and safer SAg-based immunotherapy. With its unique approach, our study is a significant step towards developing safer tumour-targeted superantigen peptides (TTSP). We identified the T-cell activation function regions on the SEA superantigen and produced variants with minimal lethality, ensuring a safer approach to cancer treatment. This involved the creation of twenty 50-amino-acid-long overlapping peptides covering the full-length SEA superantigen (P1-P20). We then screened these peptides for T-cell activation, successfully isolating two peptides (P5 and P15) with significant T-cell activation. These selected peptides were used to design and synthesise tumour-targeted superantigen peptides, which were linked to a cancer-specific third loop (L3) of transforming growth factor-α (TGF-α), TGFαL3 from either a C’ or N’ terminal with an eight-amino-acid flexible linker in between. We also produced several P15 variants by changing single amino acids or by amino acid deletions. The novel molecules were then investigated for cytokine production and tumour-targeted killing. The findings from our previous study and the current work open up new avenues for peptide-based immunotherapy, particularly when combined with other immunotherapy techniques, thereby ensuring effective and safer cancer treatment.
Specific microRNA Profile Associated with Inflammation and Lipid Metabolism for Stratifying Allergic Asthma Severity
Authors: Escolar-Peña Andrea , Delgado-Dolset María Isabel , Pablo-Torres Carmela , Tarin Carlos , Mera-Berriatua Leticia , Cuesta Apausa María del Pilar , González Cuervo Heleia , Sharma Rinku , Kho Alvin T. , Tantisira Kelan G. , McGeachie Michael J. , Rebollido-Rios Rocio , Barber Domingo , Carrillo Teresa , Izquierdo Elena , Escribese María M.
Published: August 2024

The mechanisms underlying severe allergic asthma are complex and unknown, meaning it is a challenge to provide the most appropriate treatment. This study aimed to identify novel biomarkers for stratifying allergic asthmatic patients according to severity, and to uncover the biological mechanisms that lead to the development of the severe uncontrolled phenotype. By using miRNA PCR panels, we analyzed the expression of 752 miRNAs in serum samples from control subjects (n = 15) and mild (n = 11) and severe uncontrolled (n = 10) allergic asthmatic patients. We identified 40 differentially expressed miRNAs between severe uncontrolled and mild allergic asthmatic patients. Functional enrichment analysis revealed signatures related to inflammation, angiogenesis, lipid metabolism and mRNA regulation. A random forest classifier trained with DE miRNAs achieved a high accuracy of 97% for severe uncontrolled patient stratification. Validation of the identified biomarkers was performed on a subset of allergic asthmatic patients from the CAMP cohort at Brigham and Women’s Hospital, Harvard Medical School. Four of these miRNAs (hsa-miR-99b-5p, hsa-miR-451a, hsa-miR-326 and hsa-miR-505-3p) were validated, pointing towards their potential as biomarkers for stratifying allergic asthmatic patients by severity and providing insights into severe uncontrolled asthma molecular pathways.
Loss of Heterozygosity and Mutations in the RAS-ERK Pathway Genes in Tumor Cells of Various Loci in Multiple Myeloma
Authors: Soloveva Maiia , Solovev Maksim , Risinskaya Natalya , Nikulina Elena , Yakutik Igor , Biderman Bella , Obukhova Tatiana , Chabaeva Yulia , Kulikov Sergej , Sudarikov Andrey , Mendeleeva Larisa
Published: August 2024

Multiple myeloma (MM) is a disease characterized by spatiotemporal heterogeneity of tumor clones. Different genetic aberrations can be observed simultaneously in tumor cells from different loci, and as the disease progresses, new subclones may appear. The role of liquid biopsy, which is based on the analysis of tumor DNA circulating in the blood plasma, continues to be explored in MM. Here, we present an analysis of the STR profiles and mutation status of the KRAS, NRAS, and BRAF genes, evaluated in plasma free circulating tumor DNA (ctDNA), CD138+ bone marrow cells, and plasmacytomas. The prospective single-center study included 97 patients, with a median age of 55 years. Of these, 94 had newly diagnosed symptomatic MM, and three had primary plasma cell leukemia. It should be noted that if mutations were detected only in ctDNA, “non-classical” codons were more often affected. A variety of adverse laboratory and clinical factors have been associated with the detection of rare KRAS or NRAS gene mutations in bone marrow or ctDNA, suggesting that these mutations may be factors of an unfavorable prognosis for MM. Liquid biopsy studies provide undeniable fundamental information about tumor heterogeneity and clonal evolution in MM. Moreover, we focus on using liquid biopsy to identify new high-risk factors for MM.
Obstructive Sleep Apnea and Serotoninergic Signalling Pathway: Pathomechanism and Therapeutic Potential
Authors: Witkowska Alicja , Jaromirska Julia , Gabryelska Agata , Sochal Marcin
Published: August 2024

Obstructive Sleep Apnea (OSA) is a disorder characterized by repeated upper airway collapse during sleep, leading to apneas and/or hypopneas, with associated symptoms like intermittent hypoxia and sleep fragmentation. One of the agents contributing to OSA occurrence and development seems to be serotonin (5-HT). Currently, the research focuses on establishing and interlinking OSA pathogenesis and the severity of the disease on the molecular neurotransmitter omnipresent in the human body—serotonin, its pathway, products, receptors, drugs affecting the levels of serotonin, or genetic predisposition. The 5-HT system is associated with numerous physiological processes such as digestion, circulation, sleep, respiration, and muscle tone—all of which are considered factors promoting and influencing the course of OSA because of correlations with comorbid conditions. Comorbidities include obesity, physiological and behavioral disorders as well as cardiovascular diseases. Additionally, both serotonin imbalance and OSA are connected with psychiatric comorbidities, such as depression, anxiety, or cognitive dysfunction. Pharmacological agents that target 5-HT receptors have shown varying degrees of efficacy in reducing the Apnea-Hypopnea Index and improving OSA symptoms. The potential role of the 5-HT signaling pathway in modulating OSA provides a promising avenue for new therapeutic interventions that could accompany the primary treatment of OSA—continuous positive airway pressure. Thus, this review aims to elucidate the complex role of 5-HT and its regulatory mechanisms in OSA pathophysiology, evaluating its potential as a therapeutic target. We also summarize the relationship between 5-HT signaling and various physiological functions, as well as its correlations with comorbid conditions.
Kynurenic Acid Modulates the Expression of Genes and the Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep
Authors: Misztal Tomasz , Roszkowicz-Ostrowska Katarzyna , Kowalczyk Paweł , Młotkowska Patrycja , Marciniak Elżbieta
Published: August 2024

Kynurenic acid (KYNA), a tryptophan metabolite, is believed to exert neuromodulatory and neuroprotective effects in the brain. This study aimed to examine KYNA’s capacity to modify gene expression and the activity of cellular antioxidant enzymes in specific structures of the sheep brain. Anestrous sheep were infused intracerebroventricularly with two KYNA doses—lower (4 × 5 μg/60 μL/30 min, KYNA20) and higher (4 × 25 μg/60 μL/30 min, KYNA100)—at 30 min intervals. The abundance of superoxide dismutase 2 (SOD2), catalase (CAT), and glutathione peroxidase 1 (GPx1) mRNA, as well as enzyme activities, were determined in the medial–basal hypothalamus (MBH), the preoptic (POA) area of the hypothalamus, and in the hippocampal CA1 field. Both doses of KYNA caused a decrease (p < 0.01) in the expression of SOD2 and CAT mRNA in all structures examined compared to the control group (except for CAT in the POA at the KYNA100 dose). Furthermore, lower levels of SOD2 mRNA (p < 0.05) and CAT mRNA (p < 0.01) were found in the MBH and POA and in the POA and CA, respectively, in sheep administered with the KYNA20 dose. Different stimulatory effects on GPx1 mRNA expression were observed for both doses (p < 0.05-p < 0.01). KYNA exerted stimulatory but dose-dependent effects on SOD2, CAT, and GPx1 activities (p < 0.05-p < 0.001) in all brain tissues examined. The results indicate that KYNA may influence the level of oxidative stress in individual brain structures in sheep by modulating the expression of genes and the activity of at least SOD2, CAT, and GPx1. The present findings also expand the general knowledge about the potential neuroprotective properties of KYNA in the central nervous system.