bluuebunny/crossref_metadata_2025 huggingface dataset:
Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
Authors: Devi Pinki , DeVetter Lisa , Kraft Michael , Shrestha Srijana , Miles Carol
Published: April 2022

Grafting has become a common practice for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] production in many parts of the world, due to its efficacy against biotic and abiotic stressors. However, grafting success for watermelon is challenging in part due to the complex anatomy of the cucurbit vascular system. The survival of grafted transplants depends on compatibility between the scion and rootstock, which in turn depends on anatomical, physiological, and genetic variables. A better understanding of cucurbit anatomy and graft union formation would inform grafting approaches and transplant management. An anatomical study was conducted by scanning electron microscopy (SEM) at 11 and 25 days after grafting (DAG) with seedless watermelon cultivar ‘Secretariat’ grafted onto compatible rootstock cultivars ‘Pelop’ (Lagenaria siceraria) and ‘Tetsukabuto’ (Cucurbita maxima × Cucurbita moschata) in comparison to non-grafted watermelon and rootstock seedlings. At 11 DAG, the parenchymatic cells of the central pith of grafted plants were dead and a necrotic layer was observed, representing the beginning of callus formation. New xylem strands were formed in the vascular system, connecting the rootstock with the scion. At 25 DAG, fully developed vascular bundles at the graft interface were observed with both scion-rootstock combinations. Although more studies are necessary to characterize the sequence of physiological events after grafting in Cucurbit species, this is one of the first studies to describe the complex anatomical changes that occur during watermelon graft healing.
Finding Our Bearings in Post-method Waters
Authors: Littlewood William , Wang Shufang
Published: April 2022

This paper proposes a framework to guide us in designing and implementing our classroom language pedagogy. It is based on three major principles which the teacher can keep constantly in mind: that the learners need to be engaged, that the language needs to be memorized, and that learning needs to move toward communicative competence. Each principle generates between two and four dimensions which the teacher can use to develop specific strategies.
The Moral Injury of Ineffective Police Leadership: A Perspective
Authors: Simmons-Beauchamp Bobbi , Sharpe Hillary
Published: April 2022

Research suggests that Canadian police officers are exposed to trauma at a greater frequency than the general population. This, combined with other operational stressors, such as risk of physical injury, high consequence of error, and strained resources, can leave officers less resilient to organizational stressors. In my experience, a significant and impactful organizational stressor is ineffective leadership, which include leaders who are non-supportive, inconsistent, egocentric, and morally ambiguous. Ineffective leadership in the context of paramilitary police culture has been recognized as psychologically distressing. Further, moral injury may result when leadership fails to meet officers’ needs, expectations, and values. Ineffective leadership and resulting moral injuries are an understudied area in the literature. This review will help provide a comprehensive context of policing and the impact of ineffective leadership on police mental health.
Classroom Concordancing and Second Language Motivational Self-System: A Data-Driven Learning Approach
Authors: Zare Javad , Karimpour Sedigheh
Published: April 2022

Research shows that exploring language corpora through data-driven learning (DDL) plays a significant role in language learning. Nevertheless, it is not clear if using concordancing as an application of DDL affects the learners’ second language motivation. To address this gap, the current study adopted a triangulation design, validating quantitative data model, and a quasi-experimental (comparison group, pretest-posttest) design. Ninety English-major university students with an intermediate level of English language proficiency, divided into control and experimental groups, took part in the study. Drawing on a second language motivational self-system questionnaire, the findings of the study did not result in any statistically significant differences between the students in their second language motivational self-system. Altogether, the students found learning English through a DDL approach with concordancing less motivating than receiving explicit instruction. The study has implications for language teaching and learning.
Learning in Adverse Circumstances: Impaired by Learning With Anxiety, Maladaptive Cognitions, and Emotions, but Supported by Self-Concept and Motivation
Authors: Paechter Manuela , Phan-Lesti Hellen , Ertl Bernhard , Macher Daniel , Malkoc Smirna , Papousek Ilona
Published: April 2022

The COVID-19 summer semester 2020 posed many challenges and uncertainties, quite unexpectedly and suddenly. In a sample of 314 psychology students, it was investigated how they experienced learning and preparing for an end-of-semester exam, which emotions and strain they experienced, how academic performance was affected, and how personal antecedents of learning as important facets of a learner’s identity could support or prevent overcoming adverse circumstances of learning. The participants of the study filled in a questionnaire about their achievement emotions and strain they experienced during learning and exam preparation as well as academic self-concept, motivation, gender, proneness to anxiety. Points achieved in the exam were also recorded. The interaction between the variables was investigated by a structural equation model. It showed that the investigated variables can be distinguished into two groups, variables that contribute mainly negatively to performance and variables with a positive contribution. Strain experienced during the COVID-19 pandemic and unpleasant emotions “belong together” in the sense that they inhibit academic performance directly or indirectly. Proneness to anxiety in academic situations was related with higher levels of mental, emotional, and physical disturbances due to the COVID-19 situation. In contrast, motivation and a high academic self-concept acted as support for learning and performance. Both contribute to pleasant achievement emotions in the learning situation; moreover, motivation had a direct relationship to academic performance. The results from the present study do not only provide insight into important students’ personal dispositions and their role for learning in adverse circumstances but also give advice how to strengthen students for successful learning.
Effects of Innovation, Total Quality Management, and Internationalization on Organizational Performance of Higher Education Institutions
Authors: Texeira-Quiros Joaquín , Justino Maria do Rosário , Antunes Marina Godinho , Mucharreira Pedro Ribeiro , Nunes António de Trindade
Published: April 2022

The aim of this research is to analyze the effects of innovation strategies, Total Quality Management (TQM) dimensions, and internationalization strategies that Higher Education Institutions (HEIs) might adopt, and their effects on their organizational performance. Due to globalization and the constant changes and demands that have taken place today, HEIs are forced to seek new quality assurance instruments in higher education, to ensure greater competitiveness in the markets and their survival. To examine the association between the independent variables, namely, TQM dimensions, innovation strategies, and internationalization strategies with the dependent variable, that is organizational performance of HEIs, we have chosen to use multiple linear regression analysis. A nine-predictor multiple linear regression model was proposed. The nine predictor variables are Communication, Involvement/teacher empowerment, Development/Teacher training, Continuous improvement, Leadership/Administration’s Commitment, Data analysis/Measurement of results, Focus on students, Innovation Strategies, and Internationalization strategy. We conclude that some TQM variables have a significant association with the organizational performance of HEIs, namely, Involvement/teacher empowerment, and Development/teacher training. On the other hand, also the Innovation strategies and Internationalization strategy have a significant association with the organizational performance of HEIs. This research is of enormous importance for the study of HEIs, considering their role in the development of any country and its impact on society as creators of knowledge and science. Since these institutions increasingly must deal with extremely competitive market environments, knowledge of the factors that can assist in increasing the organizational performance of HEIs is of great relevance.
Positive Mental Health Literacy: A Concept Analysis
Authors: Carvalho Daniel , Sequeira Carlos , Querido Ana , Tomás Catarina , Morgado Tânia , Valentim Olga , Moutinho Lídia , Gomes João , Laranjeira Carlos
Published: April 2022

Background

The positive component of Mental Health Literacy (PMeHL) refers to a person’s awareness of how to achieve and maintain good mental health. Although explored recently, the term still lacks a clear definition among healthcare practitioners.

Aim

To identify the attributes and characteristics of PMeHL, as well as its theoretical and practical applications.

Methods

Literature search (using the Medline and CINAHL databases) and review, covering the last 21 years, followed by concept analysis according to the steps described by Walker and Avant approach.

Results

Positive component of Mental Health Literacy is considered one component of MHL, integrating positive mental health. The concept’s attributes include: (a) competence in problem-solving and self-actualization; (b) personal satisfaction; (c) autonomy; (d) relatedness and interpersonal relationship skills; (e) self-control; and (f) prosocial attitude. Four case scenarios (model, borderline, related and contrary cases) were used to clarify the antecedents (individual factors and social/contextual factors) and consequences (individual sphere; relational/social sphere; contextual/organizational sphere) of PMeHL.

Conclusion

Positive component of Mental Health Literacy is considered a component of MHL, which deserves attention throughout the lifespan, in different contexts and intervention levels. Considering PMeHL as a multi-faceted and dynamic construct will help understand the mechanisms that improve mental health and promote healthy behaviors. Priority should be given to robust primary research focused on nursing interventions that enhance and sustain PMeHL in people and families.
Neuronal Cell Adhesion Molecules May Mediate Neuroinflammation in Autism Spectrum Disorder
Authors: Eve Madeline , Gandawijaya Josan , Yang Liming , Oguro-Ando Asami
Published: April 2022

Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by restrictive and repetitive behaviors, alongside deficits in social interaction and communication. The etiology of ASD is largely unknown but is strongly linked to genetic variants in neuronal cell adhesion molecules (CAMs), cell-surface proteins that have important roles in neurodevelopment. A combination of environmental and genetic factors are believed to contribute to ASD pathogenesis. Inflammation in ASD has been identified as one of these factors, demonstrated through the presence of proinflammatory cytokines, maternal immune activation, and activation of glial cells in ASD brains. Glial cells are the main source of cytokines within the brain and, therefore, their activity is vital in mediating inflammation in the central nervous system. However, it is unclear whether the aforementioned neuronal CAMs are involved in modulating neuroimmune signaling or glial behavior. This review aims to address the largely unexplored role that neuronal CAMs may play in mediating inflammatory cascades that underpin neuroinflammation in ASD, primarily focusing on the Notch, nuclear factor-κB (NF-κB), and mitogen-activated protein kinase (MAPK) cascades. We will also evaluate the available evidence on how neuronal CAMs may influence glial activity associated with inflammation. This is important when considering the impact of environmental factors and inflammatory responses on ASD development. In particular, neural CAM1 (NCAM1) can regulate NF-κB transcription in neurons, directly altering proinflammatory signaling. Additionally, NCAM1 and contactin-1 appear to mediate astrocyte and oligodendrocyte precursor proliferation which can alter the neuroimmune response. Importantly, although this review highlights the limited information available, there is evidence of a neuronal CAM regulatory role in inflammatory signaling. This warrants further investigation into the role other neuronal CAM family members may have in mediating inflammatory cascades and would advance our understanding of how neuroinflammation can contribute to ASD pathology.
Multi-Level Social Capital and Subjective Wellbeing Among the Elderly: Understanding the Effect of Family, Workplace, Community, and Society Social Capital
Authors: Xu Zongyou , Zhang Wenjie , Zhang Xuewen , Wang Yixi , Chen Qing , Gao Bo , Li Ningxiu
Published: April 2022

Background

Maintaining the subjective wellbeing of the elderly people is one of the major concerns in promoting health aging. This study concerned the influence of multi-level social capital on subjective welling and explored the affecting path among the elderly.

Methods

A total of 1,078 elderly individuals anonymously and effectively surveyed in 2018, data was collected including their family, workplace, community, society social capital and subjective wellbeing, we used the structural equation modeling to test the hypothesis relationships among the variables.

Results

We found that the total score of subjective wellbeing among the aging participants was 72.36 ± 10.08 on a range of 0–100. Family (β = 0.151, P < 0.001), workplace (β = 0.090, P < 0.001), community (β = 0.163, P < 0.001) social capital had a direct positive effect on subjective wellbeing. Society social capital had a direct positive effect on family (β = 0.253, P < 0.001), workplace (β = 0.585, P < 0.001), community (β = 0.438, P < 0.001) social capital. And society social capital had an indirect positive effect on subjective wellbeing through the mediating role of family, workplace, and community social capital.

Conclusion

The research demonstrated that all the micro, meso and macro levels of social capital have protective effects for subjective wellbeing through direct or indirect way, inspiring to provide continuous improvement measures for multi-level social capital aimed at the elderly people.
Urban-Rural Differences: The Impact of Social Support on the Use of Multiple Healthcare Services for Older People
Authors: Chi Zhang , Han Hu
Published: April 2022

Background

There are many kinds of chronic diseases, high incidence and high hospitalization rate in older people caused by population aging. The increasing demand for healthcare services has become an increasingly prominent problem in Chinese society. The purpose of this paper is to explore the influence of social support on multiple healthcare services for older people and the urban-rural differences among them.

Methods

The data are from our field survey in Shaanxi province in 2019. Using the Anderson model as the basic explanatory framework, this paper uses the Tobit-model to analyses the impact of social support for older people on the utilization of therapeutic healthcare services, and the Logit-model to analyze the impact of living arrangements and social support on the utilization of preventive healthcare services for older people.

Results

This paper examines the impact of formal and informal support on outpatient, inpatient, and preventive healthcare services for older people, provides an in-depth analysis of the differences in the impact of social support on healthcare service utilization between urban and rural older people and analyses the contribution of various factors to the impact. The coefficient effect is divided into two parts: the coefficient “premium” of urban older people relative to urban and rural older people as a whole, which accounts for 10.8% of the total difference; and the “premium” of urban and rural older people as a whole to rural older people, which accounts for 18.9% of the total difference. The coefficient effect accounts for 29.7 per cent of the total difference.

Conclusions

Rural older people place greater importance on the quantity of formal social support, while urban older people place greater importance on the quality of formal social support. The phenomenon of raising children for old age was evident in the use of healthcare services by rural older people, while the phenomenon of distant relatives being better than close neighbors was evident in the use of healthcare services by urban older people. Free preventive healthcare services in rural China have largely compensated for the lack of health benefits for rural older people.
The Prevalence of Irritable Bowel Syndrome Among Chinese University Students: A Systematic Review and Meta-Analysis
Authors: Yang Weixin , Yang Xiao , Cai Xianghao , Zhou Zhuoren , Yao Huan , Song Xingrong , Zhao Tianyun , Xiong Peng
Published: April 2022

Background

Irritable bowel syndrome (IBS) has become a common public health issue among university students, impairing their physical and mental health. This meta-analysis aimed to examine the pooled prevalence of IBS and its associated factors among Chinese university students.

Methods

Databases of PubMed, EMBASE, MEDLINE (via EBSCO), CINAHL (via EBSCO), Wan Fang, CNKI and Weipu (via VIP) were systematically searched from inception date to May 31, 2021. Meta-analysis was performed using random-effects models. Meta-regression and subgroup analysis were used to detect the potential source of heterogeneity.

Key Results

A total of 22 cross-sectional studies (14 were in Chinese and 8 were in English) with 33,166 Chinese university students were included. The pooled prevalence of IBS was estimated as 11.89% (95% CI = 8.06%, 16.35%). The prevalence was 10.50% (95% CI = 6.80%, 15.87%) in Rome II criteria, 12.00% (95% CI = 8.23%, 17.17%) in Rome III criteria, and 3.66% (95% CI = 2.01%, 6.60%) in Rome IV criteria. The highest prevalence of IBS was 17.66% (95% CI = 7.37%, 36.64%) in North China, and the lowest was 3.18% (95% CI = 1.28%, 7.68%) in South China. Subgroup analyses indicated that gender, major, anxiety and depression symptoms, drinking and smoking behaviors were significantly associated with the prevalence of IBS. Meta-regression analyses suggested that region influenced prevalence estimates for IBS.

Conclusions and Inferences

This meta-analysis illustrated that IBS is very common in Chinese university students. Regular screening, effective prevention, and appropriate treatments should be implemented to reduce the risk of IBS in this population. More future studies should be conducted in Northeastern and Southwestern parts of China.
Semantic Representations for NLP Using VerbNet and the Generative Lexicon
Authors: Brown Susan Windisch , Bonn Julia , Kazeminejad Ghazaleh , Zaenen Annie , Pustejovsky James , Palmer Martha
Published: April 2022

The need for deeper semantic processing of human language by our natural language processing systems is evidenced by their still-unreliable performance on inferencing tasks, even using deep learning techniques. These tasks require the detection of subtle interactions between participants in events, of sequencing of subevents that are often not explicitly mentioned, and of changes to various participants across an event. Human beings can perform this detection even when sparse lexical items are involved, suggesting that linguistic insights into these abilities could improve NLP performance. In this article, we describe new, hand-crafted semantic representations for the lexical resource VerbNet that draw heavily on the linguistic theories about subevent semantics in the Generative Lexicon (GL). VerbNet defines classes of verbs based on both their semantic and syntactic similarities, paying particular attention to shared diathesis alternations. For each class of verbs, VerbNet provides common semantic roles and typical syntactic patterns. For each syntactic pattern in a class, VerbNet defines a detailed semantic representation that traces the event participants from their initial states, through any changes and into their resulting states. The Generative Lexicon guided the structure of these representations. In GL, event structure has been integrated with dynamic semantic models in order to represent the attribute modified in the course of the event (the location of the moving entity, the extent of a created or destroyed entity, etc.) as a sequence of states related to time points or intervals. We applied that model to VerbNet semantic representations, using a class's semantic roles and a set of predicates defined across classes as components in each subevent. We will describe in detail the structure of these representations, the underlying theory that guides them, and the definition and use of the predicates. We will also evaluate the effectiveness of this resource for NLP by reviewing efforts to use the semantic representations in NLP tasks.
Group Cohesion: The Glue That Helps Teams Stick Together
Authors: Eys Mark , Coleman Taylor , Crickard Travis
Published: April 2022

Playing together with other people can be an extremely fun aspect of taking part in sports. It can also be challenging when some people are not team players. This article focuses on the topic of group cohesion, which we describe as the glue that helps teammates to stick together. We might also define cohesion as the amount of unity or harmony in a team. Sport teams can be cohesive in terms of how well they play together during practices and games (i.e., task cohesion) as well as how well they get along away from their sport (i.e., social cohesion). Both types of cohesion are important because they lead to better individual and team performance, and athletes are more likely to be happy with playing on the team and to continue taking part. We suggest simple strategies that you and your coaches can use to help your team become more cohesive over time.
Identification of microRNA Transcriptome Involved in Bovine Intramuscular Fat Deposition
Authors: Duckett Susan K. , Greene Maslyn A.
Published: April 2022

Background

Intramuscular fat deposition in beef is a major determinant of carcass quality and value in the USA. The objective of this study was to examine changes in microRNA (miRNA) transcriptome that are involved with intramuscular fat deposition with time-on-concentrates (TOC). Yearling steers were individually fed a high concentrate diet and changes in intramuscular fat deposition were monitored by real-time ultrasound at 28 to 33 d intervals. Longissimus muscle biopsies collected on d 0, 92 and 124 TOC to examine changes in miRNA transcriptome that are involved in intramuscular fat deposition.

Results

Steer body weight increased (P < 0.0001) at each weigh day during TOC. Fat thickness increased (P < 0.005) from d 28 to 124. Ribeye area was larger (P < 0.001) on d 124 than d 61, which was larger than d 0 and 28. Ultrasound intramuscular fat content was greater (P < 0.001) on d 92 and 124 compared to d 0, 28 or 61. Sequencing of the muscle biopsy samples identified one miRNA, bta-miR-122, that was up-regulated (P < 0.005) at d 92 and 124 compared to d 0. At d 92 TOC, mRNA expression levels of fatty acid binding protein 4 (FABP4) and elongase 6 (ELOVL6) were up-regulated (P < 0.01) compared to d 0; whereas at d 124, lipogenic genes involved in de novo fatty acid synthesis, fatty acid transport, elongation and desaturation were highly up-regulated compared to d0.

Conclusions

Small RNA sequencing identified bta-miR-122 as a potential miRNA of interest that may be involved in intramuscular fat deposition with increasing TOC. Increased intramuscular fat content, as measured by real-time ultrasound, combined with differential gene expression suggests that preadipocyte differentiation may be stimulated first, which is followed by a global up-regulation of lipogenic genes involved in de novo fatty acid synthesis that provide fatty acids for subsequent hypertrophy.
Combustion Characteristics of a Supersonic Combustor with a Large Cavity Length-to-Depth Ratio
Authors: Li Xiang , Lei Qingchun , Zhao Xiaocun , Fan Wei , Chen Shuang , Chen Li , Tian Ye , Zhou Quan
Published: April 2022

The combustion characteristics of a hydrogen-fueled supersonic combustor featuring a large cavity length-to-depth ratio (i.e., 11) were examined by performing experimental trials while varying the fuel injector positions and equivalence ratios. During these trials, flame chemiluminescence images were acquired simultaneously from the side and bottom of the combustor under Mach 2.0 inflow conditions. The flame was observed to stabilize inside the cavity under all conditions. Proper orthogonal decomposition (POD) and dynamic mode decomposition (DMD) analyses of sequential flame chemiluminescence images demonstrated the important effects of oblique shocks induced by fuel injection and heat release on flame stabilization. Because fluctuations in the locations of the flame and of the intense heat release zone were not observed and no dominant frequency was identified in POD and DMD analyses, the present configuration was evidently able to suppress combustion instability. The present research provides preliminary guidance for exploring the feasibility of using cavity combustors with large length-to-depth ratios in scramjet engines.
Probability Analysis of Widespread Fatigue Damage in LY12-CZ Aluminum Alloy Single-Row Seven-Hole Plate
Authors: Liu Kai , Wang Fangli , Pan Wei , Yang Le , Bai Shuwei , Zhu Qiang , Tong Mingbo
Published: April 2022

In order to determine the average behavior time of widespread fatigue damage (WFD) in an aircraft multi-site damage (MSD) structure and complete the fatigue analysis of WFD sensitive parts, this paper adopts a probabilistic method to analyze the crack initiation and crack propagation of a single row of collinear seven-hole plates. The simulation analysis of the whole process from crack initiation to structural failure is realized. First, through statistical analysis of the test data of the LY12-CZ alloy single-detail plate with hole, the probability distribution of crack initiation life and growth rate is obtained, and this probability distribution is expressed by the randomization of variables. Then, using the related theories of fracture mechanics and fatigue statistics, the whole process of initiation, propagation, and connection of multiple cracks in the MSD structure to the occurrence of WFD was realized through the Monte Carlo method. Finally, a group of single-row seven-hole plate examples are used to verify the accuracy of the calculation results. The results show that the calculated results in this paper are in good agreement with the experimental data, and can accurately predict the life of MSD structures under a certain reliability.
Establishment and Application of Multiplex PCR Systems Based on Molecular Markers for HMW-GSs in Wheat
Authors: Yao Chuxuan , Zhang Cuimian , Bi Caili , Zhou Shuo , Dong Fushuang , Liu Yongwei , Yang Fan , Jiao Bo , Zhao He , Lyu Mengyu , Wang Haibo , Chai Jianfang
Published: April 2022

High-molecular-weight glutenin subunits (HMW-GSs) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique end-use quality properties of common wheat (Triticum aestivum L.). Wheat accessions with the high-quality HMW-GSs combination of Ax2*/Bx7OE/Dx5 usually exhibit strong gluten characteristics. In order to stack these three high-quality subunit genes by molecular markers in strong gluten wheat breeding, an agarose gel-based multiplex PCR marker for these high-quality HMW-GSs and two agarose gel-based multiplex PCR markers detecting the homozygosity of Ax2* and Bx7OE subunits were developed. These markers were verified in an F2 segregating population from a cross between a medium-gluten winter wheat cultivar with the HMW-GSs combination of Ax null/Bx7 + By8/Dx4 + Dy12 and a strong-gluten spring wheat cultivar with the HMW-GSs combination of Ax2*/Bx7OE + By8*/Dx5 + Dy10. By integrating the newly established multiplex PCR markers and a published co-dominant PCR marker of the Dx5 subunit, a complete molecular marker selection system was established. After multiple rounds of molecular marker-assisted selection with the system, 17 homozygous winter wheat lines that stacked the three high-quality HMW-GSs were generated. The gluten strength of these homozygous lines was comparable to their strong-gluten parent, but significantly higher than that of their medium-gluten parent by measuring their lactic acid-sodium dodecyl sulfate solvent retention capacities of whole wheat meal. The multiplex PCR systems established in the present study can be used for molecular marker-assisted selection of strong gluten wheats.
The Dynamics of Molybdenum, Boron, and Iron Uptake, Translocation and Accumulation by Pea (Pisum sativum L.)
Authors: Wysokinski Andrzej , Lozak Izabela , Kuziemska Beata
Published: April 2022

Molybdenum (Mo), boron (B), and iron (Fe) play an important role in symbiotic nitrogen fixation by legume plants. The intensity of this process varies in different growth stages of legumes, and the changes are accompanied by changes in the content and translocation of these micronutrients in the plant. A two-year field experiment was conducted to investigate the dynamics of molybdenum, boron, and iron content, translocation, and accumulation in pea plants. Two pea cultivars were studied in six stages of growth, from the four-leaf stage to full maturity. The content of Mo, B, and Fe in the roots of pea was highest from the four-leaf stage to the full flowering stage, i.e., the period of establishment of symbiosis and the most intensive atmospheric nitrogen fixation. The bioaccumulation factors of Mo and Fe were generally highest in the initial stages of pea growth and decreased during generative development, while the reverse pattern was observed for boron. The bioaccumulation factors also indicate high bioaccumulation of Mo and B and low bioaccumulation of Fe in the biomass of pea. The translocation factor indicated a high potential for allocation of Mo from the roots to the aerial parts, increasing during growth; high and stable potential for allocation of boron; and very minor allocation of iron to the aerial parts. The values of all parameters tested were usually dependent on the conditions in which the experiment was conducted (the year), but not on the cultivar of a pea.
Assessment of Fertilizer Quality in Horse Waste-Based Bokashi Fertilizer Formulations
Authors: Gashua Auwalu Garba , Sulaiman Zulkefly , Yusoff Martini Mohammad , Samad Mohd Yusoff Abd , Ramlan Mohd Fauzi , Salisu Monsuru Adekunle
Published: April 2022

Agricultural waste is a type of solid waste that needs to be managed properly. Organic waste can be recycled to produce bokashi fertilizer, which can be used to improve soil health, increase crop production, and sanitize the environment. However, it may contain heavy metals that could be toxic to plants and can pollute the environment if not properly decomposed. This study was designed to evaluate the fertilizer quality of six different bokashi fertilizer ratios (bfrs) over seven- and thirty-day maturation periods. The raw materials used include horse bedding waste (HBW), cow dung (CD), and paddy husk charcoal (PHC) in different ratios, treated with an effective microorganisms (EM4) solution. All the nutrients studied (N, P, K, Mg, and Ca) were significantly affected by the bokashi fertilizer ratios (bfrs). The best bokashi fertilizer ratio was bokashi fertilizer ratio-6 (bfr6), but it was statistically similar to bokashi fertilizer ratio-5 (bfr5). Its N, P, K, Mg, and Ca contents were higher than the control (bfr1) by 133.9%, 225.5%, 196.4%, 105.0%, and 84.7%, respectively. Similarly, all these nutrients were significantly affected by time. N, P, K, and Mg increased by 21.2, 33.0%, 16.4%, and 28.8%, respectively, after 30 days of maturation, with a decrease in Ca only 2.4%, which was not significant A germination index (GI) of 90.1% was obtained using cabbage seeds. The heavy metals result and germination bioassay confirmed the safety and maturity of the bokashi fertilizer. In conclusion, the results revealed that good-quality bokashi fertilizer can be produced within 30 days. Bfrr5 and bfr6 are equally good candidates for producing good-quality bokashi fertilizer for effective crop growth.
The Development of an Electric-Driven Control System for a High-Speed Precision Planter Based on the Double Closed-Loop Fuzzy PID Algorithm
Authors: Wang Weiwei , Wu Kunpeng , Zhang Yin , Wang Minxue , Zhang Chunling , Chen Liqing
Published: April 2022

In order to solve the problems of traditional seeders, such as low seeding efficiency, tangled straw, a large amount of clay, easy ridge breakage in sowing operations, low qualified rate of high-speed seeding, and poor uniformity, this paper takes the pneumatic corn planter as the research object, the Beidou automatic driving unit as the carrier, the CAN (Controller Area Network) bus as the communication medium, and the double closed-loop fuzzy PID (proportion-integral-derivative) algorithm as the control core and designs a high-speed precision corn seeding control system based on Beidou navigation. It solves the problems that exist in traditional planters. In the bench experiment, the stability of the system is judged by comparing the motor control accuracy with ordinary PID and measuring the motor response time of the system at different speeds. The bench test results show that when the theoretical seeding speed is 0~34 r·min−1, the response time of the motor is shortened by 0.51 s compared with the ordinary PID control, and the error between the actual speed and the target value is less than 0.35%. The field experiment results show that when the unit runs for 5~13 km·h−1, the qualified rate of average planting spacing is greater than 95.81%, the reseeding rate is less than 10.11%, and the coefficient of variation is less than 16.72%, which complies with the standard of a corn sowing operation.
Comparative Analysis of miRNA Expression Profiles in Skeletal Muscle of Bian Chickens at Different Embryonic Ages
Authors: Zhou Kai-Zhi , Wu Peng-Fei , Zhang Xin-Chao , Ling Xuan-Ze , Zhang Jin , Zhang Li , Li Pei-Feng , Zhang Tao , Wei Qing-Yu , Zhang Gen-Xi
Published: April 2022

MicroRNAs (miRNAs) are widely involved in the growth and development of skeletal muscle through the negative regulation of target genes. In order to screen out the differentially expressed miRNAs (DEMs) associated with skeletal muscle development of Bian chickens at different embryonic ages, we used the leg muscles of fast-growing and slow-growing Bian chickens at the 14th and 20th embryonic ages (F14, F20, S14 and S20) for RNA-seq. A total of 836 known miRNAs were identified, and 121 novel miRNAs were predicted. In the F14 vs. F20 comparison group, 127 DEMs were screened, targeting a total of 2871 genes, with 61 miRNAs significantly upregulated and 66 miRNAs significantly downregulated. In the S14 vs. S20 comparison group, 131 DEMs were screened, targeting a total of 3236 genes, with 60 miRNAs significantly upregulated and 71 miRNAs significantly downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the predicted target genes were significantly enriched in 706 GO terms and 6 KEGG pathways in the F14 vs. F20 group and 677 GO terms and 5 KEGG pathways in the S14 vs. S20 group. According to the interaction network analysis, we screened five coexpressed DEMs (gga-miR-146a-3p, gga-miR-2954, gga-miR-34a-5p, gga-miR-1625-5p and gga-miR-18b-3p) with the highest connectivity degree with predicted target genes between the two comparison groups, and five hub genes (HSPA5, PKM2, Notch1, Notch2 and RBPJ) related to muscle development were obtained as well. Subsequently, we further identified nine DEMs (gga-let-7g-3p, gga-miR-490-3p, gga-miR-6660-3p, gga-miR-12223-5p, novel-miR-327, gga-miR-18a-5p, gga-miR-18b-5p, gga-miR-34a-5p and gga-miR-1677-3p) with a targeting relationship to the hub genes, suggesting that they may play important roles in the muscle development of Bian chickens. This study reveals the miRNA differences in skeletal muscle development between 14- and 20-day embryos of Bian chickens from fast- and slow-growing groups and provides a miRNA database for further studies on the molecular mechanisms of the skeletal muscle development in Bian chickens.
Effects of Different Selenium Sources on the Laying Performance, Egg Quality, Antioxidant, and Immune Responses of Laying Hens under Normal and Cyclic High Temperatures
Authors: Wang Weihan , Kang Ruifen , Liu Meiling , Wang Zhong , Zhao Lihong , Zhang Jianyun , Huang Shimeng , Ma Qiugang
Published: April 2022

The aim of this study was to evaluate the effects of different selenium (Se) sources on the laying performance, egg quality, antioxidant, and immune responses of laying hens under different temperatures. In an 8-week experiment, a total of 480 44-week-old laying hens were randomly divided into 8 groups, with 6 replicates for each group and 10 hens per replicate, and fed with a basal diet (BK), basal diet with 0.3 mg/kg of Se from sodium selenite (SS), from Se yeast (SY), or from selenium-enriched yeast culture (SYC) under normal temperature (NT, 26 ± 2 °C) and cyclic high temperature (CHT, 26 ± 2 °C~33 ± 2 °C). CHT decreased the laying performance and serum levels of Se, immunoglobulin G (IgG), and interleukin-10 (IL-10), and significantly increased the serum free triiodothyronine (FT3), deiodinase-I (DI-I), and heat stress protein (HSPs) (p < 0.05). In addition, SYC increased the egg yolk color, and SS increased serum IgG level. SS, SY, and SYC reduced the level of interleukin-6 (IL-6) (p < 0.05). In conclusion, Se can increase egg yolk color, antioxidant capacity, and immune capacity under heat stress, and the effect of organic Se is better than that of inorganic Se.
Effect of Dietary Laminaria digitata with Carbohydrases on Broiler Production Performance and Meat Quality, Lipid Profile, and Mineral Composition
Authors: Costa Mónica M. , Pestana José M. , Osório Diogo , Alfaia Cristina M. , Martins Cátia F. , Mourato Miguel , Gueifão Sandra , Rego Andreia M. , Coelho Inês , Coelho Diogo , Lemos José P. C. , Fontes Carlos M. G. A. , Lordelo Madalena M. , Prates José A. M.
Published: April 2022

We hypothesized that dietary inclusion of 15% Laminaria digitata, supplemented or not with carbohydrases, could improve the nutritional value of poultry meat without impairing animal growth performance. A total of 120 22-day old broilers were fed the following dietary treatments (n = 10) for 14 days: cereal-based diet (control); control diet with 15% L. digitata (LA); LA diet with 0.005% Rovabio® Excel AP (LAR); LA diet with 0.01% alginate lyase (LAE). Final body weight was lower and feed conversion ratio higher with LA diet than with the control. The ileal viscosity increased with LA and LAR diets relative to control but without differences between LAE and control. The pH of thigh meat was higher, and the redness value of breast was lower with LA diet than with control. Meat overall acceptability was positively scored for all treatments. The γ-tocopherol decreased, whereas total chlorophylls and carotenoids increased in meat with alga diets relative to control. The percentage of n-3 polyunsaturated fatty acids (PUFA) and accumulation of bromine and iodine in meat increased with alga diets compared with control. Feeding 15% of L. digitata to broilers impaired growth performance but enhanced meat quality by increasing antioxidant pigments, with beneficial effects on n-3 PUFA and iodine.
Genetic Parameters of Somatic Cell Score in Florida Goats Using Single and Multiple Traits Models
Authors: Jimenez-Granado Rocío , Molina Antonio , Ziadi Chiraz , Sanchez Manuel , Muñoz-Mejías Eva , Demyda-Peyrás Sebastián , Menendez-Buxadera Alberto
Published: April 2022

A total of 1,031,143 records of daily dairy control test of Spanish Florida goats were used for this study. The database was edited, and only the records of the first three lactations were kept. The final database contained 340,654 daily-test somatic cell counts from 27,749 daughters of 941 males and 16,243 goats. The evolution of this count in the last 14 years was analyzed following French and American international associations’ criteria for the risk of mastitis in goats, and confirmed the slight increase in SCS in the last years and the importance of this problem (50% of dairy control tests show a risk of suffering mastitis). For the genetic analysis, the SCS records were log-transformed to normalize this variable. Two strategies were used for the genetic analysis: a univariate animal model for the SCS assuming that SCS does not vary throughout the parities, and a multi-character animal model, where SCS is not considered as the same character in the different parities. The heritabilities (h2) were higher in the multiple traits models, showings an upward trend from the first to the third parity (h2 between 0.245 to 0.365). The genetic correlations of the same trait, as well as between breeding values (GVs) between different parities, were different from unity. The breeding values (EBVs) obtained for both models were subjected to a PCA: the first eigenvector (λ1) explained most of the variations (between 74% to 90%), while the second λ2 accounted for between 9% to 20% of the variance, which shows that the selection will be proportionally favorable but not equivalent in all parities and that there are some variations in the type of response.
Impact of Maternal Feed Restriction at Different Stages of Gestation on the Proteomic Profile of the Newborn Skeletal Muscle
Authors: Costa Thaís , Dutra Luana , Mendes Tiago , dos Santos Marta , Veroneze Renata , Gionbelli Mateus , Duarte Marcio
Published: April 2022

We aimed to investigate the effects of the maternal plane of nutrition during gestation on the proteome profile of the skeletal muscle of the newborn. Pregnant goats were assigned to the following experimental treatments: restriction maintenance (RM) where pregnant dams were fed at 50% of their maintenance requirements from 8–84 days of gestation, and then feed of 100% of the maintenance requirements was supplied from 85—parturition (n = 6); maintenance restriction (MR) where pregnant dams were fed at 100% of their maintenance requirements from 8–84 days of gestation, and then experienced feed restriction of 50% of the maintenance requirements from 85—parturition (n = 8). At birth, newborns were euthanized and samples of the Longissimus dorsi muscle were collected and used to perform HPLC-MS/MS analysis. The network analyses were performed to identify the biological processes and KEGG pathways of the proteins identified as differentially abundant protein and were deemed significant when the adjusted p-value (FDR) < 0.05. Our results suggest that treatment RM affects the energy metabolism of newborns’ skeletal muscle by changing the energy-investment phase of glycolysis, in addition to utilizing glycogen as a carbon source. Moreover, the RM plane of nutrition may contribute to fatty acid oxidation and increases in the cytosolic α-KG and mitochondrial NADH levels in the skeletal muscle of the newborn. On the other hand, treatment MR likely affects the energy-generation phase of glycolysis, contributing to the accumulation of mitochondrial α-KG and the biosynthesis of glutamine.
Lamellar Keratoplasty Using Acellular Bioengineering Cornea (BioCorneaVetTM) for the Treatment of Feline Corneal Sequestrum: A Retrospective Study of 62 Eyes (2018–2021)
Authors: Xu Huihao , Sapienza John S. , Jin Yipeng , Lin Jiahao , Zheng Xiaobo , Dong Haodi , Diao Hongxiu , Zhao Ying , Gao Jiafeng , Tang Jing , Feng Xueqian , Micceri Danielle , Zeng Haoran , Lin Degui
Published: April 2022

To retrospectively evaluate the effectiveness and outcome of lamellar keratoplasty using acellular bioengineering cornea (BioCorneaVetTM) for the treatment of feline corneal sequestrum (FCS). The medical records of cats diagnosed with FCS that underwent lamellar keratoplasty with BioCorneaVetTM between 2018 and 2021 with a minimum of 3 months of follow-up were reviewed. Follow-up examinations were performed weekly for 3 months, and then optical coherence tomography (OCT) examination was performed on select patients at 0, 3, 6, and 12 months post-operatively. A total of 61 cats (30 left eyes and 32 right eyes) were included. The Persian breed was overrepresented, 48/61 (78.69%). Four different thicknesses of acellular bioengineering cornea were used (200, 300, 400, or 450 microns), and the mean graft size was 8.23 mm (range, 5.00–12.00 mm). Minor complications were composed of partial dehiscence, and protrusion of the graft occurred in 7/62 eyes (11.29%). The median postoperative follow-up was 12.00 months (range, 3–41 months). A good visual outcome was achieved in 60/62 eyes (96.77%), and a mild to moderate corneal opacification occurred in 2/62 (3.23%). No recurrence of corneal sequestrum was observed. From the results, lamellar keratoplasty using acellular bioengineering cornea (BioCorneaVetTM) is an effective treatment for FCS, providing a good tectonic support and natural collagen framework, and resulting in satisfactory visual and cosmetic effects.
Acknowledging the Relevance of Elephant Sensory Perception to Human–Elephant Conflict Mitigation
Authors: Ball Robbie , Jacobson Sarah L. , Rudolph Matthew S. , Trapani Miranda , Plotnik Joshua M.
Published: April 2022

Elephants are well known for their socio-cognitive abilities and capacity for multi-modal sensory perception and communication. Their highly developed olfactory and acoustic senses provide them with a unique non-visual perspective of their physical and social worlds. The use of these complex sensory signals is important not only for communication between conspecifics, but also for decisions about foraging and navigation. These decisions have grown increasingly risky given the exponential increase in unpredictable anthropogenic change in elephants’ natural habitats. Risk taking often develops from the overlap of human and elephant habitat in Asian and African range countries, where elephants forage for food in human habitat and crop fields, leading to conflict over high-quality resources. To mitigate this conflict, a better understanding of the elephants’ sensory world and its impact on their decision-making process should be considered seriously in the development of long-term strategies for promoting coexistence between humans and elephants. In this review, we explore the elephants’ sensory systems for audition and olfaction, their multi-modal capacities for communication, and the anthropogenic changes that are affecting their behavior, as well as the need for greater consideration of elephant behavior in elephant conservation efforts.