bluuebunny/crossref_metadata_2025 huggingface dataset:
Drying Behavior of Hardwood Components (Sapwood, Heartwood, and Bark) of Red Oak and Yellow-Poplar
Authors: Rahimi Sohrab , Singh Kaushlendra , DeVallance David , Chu Demiao , Bahmani Mohsen
Published: May 2022

This paper presents differences in the drying behavior of red oak and yellow-poplar sapwood, heartwood, and bark and their relationship with selected physical characteristics. Drying experiments were performed on samples of sapwood, heartwood, and bark of respective species at 105 °C under nitrogen conditions. In addition, physical characteristics such as green moisture content, specific gravity, volumetric shrinkage, shrinkage of the cell wall, total porosity, pore volume occupied by water, and specific pore volume were calculated. The results showed that the volumetric and cellular shrinkages of sapwood were greater than those of heartwood for both species. For red oak, the specific gravity of sapwood and heartwood was not significantly different. Additionally, the total porosity of heartwood was lower than that of sapwood in red oak. The results also indicated that yellow-poplar dried faster than red oak. Among all three components, bark dried faster than sapwood and heartwood in both species. The activation energy for sapwood drying was less than for heartwood drying.
Fire as a Major Factor in Dynamics of Tree-Growth and Stable δ13C and δ18O Variations in Larch in the Permafrost Zone
Authors: Knorre Anastasia A. , Siegwolf Rolf T. W. , Kirdyanov Alexander V. , Saurer Matthias , Churakova (Sidorova) Olga V. , Prokushkin Anatoly S.
Published: May 2022

Wildfires are one of the most important environmental factors controlling forest ecosystem physiology and the carbon balance in the permafrost zone of North Siberia. We investigated tree-ring width (TRW) and stable isotope chronologies in tree-ring cellulose (δ13CCell, δ18OCell) of Larix Gmelinii (Rupr.) Rupr. from a wet (WS) and a dry (DS) site. These sites are characterized by different fire histories (fire in 1852 at the wet and 1896 at the dry sites, respectively). TRW and δ18OCell are identified to be the most sensitive parameters in the changing tree growth conditions after fire. The differences in the soil seasonal thermal regime of sites after fires are shown in the relationship between the studied parameters. The δ13CCell values in tree rings from the two sites are positively correlated independently of the fire impact. This fact indicates that δ13CCell chronologies might be more adequate for climatic reconstruction in the region due to the climate signal consistency. Relationships of δ18OCell values between the two sites are still significantly positive 60 years after the fire impact. Dendroclimatic analysis indicates significant changes in tree-ring growth and isotopic ratio responses to climate due to the increased demand of water for trees during the post-fire period (deeper seasonal subsidence of permafrost).
Protection against Osteoporosis by Fermented Mulberry Vinegar Supplementation via Inhibiting Osteoclastic Activity in Ovariectomized Rats and Osteoclastic Cells
Authors: Yim Eun Jung , Jo Seung Wha , Kang Hyeon Jin , Park Seul Ki , Yu Kang Yeol , Jeong Do-Youn , Park Sunmin
Published: May 2022

Menopause increases the osteoporosis risk, to which phytoestrogen intake can be beneficial. This study hypothesized that mulberry vinegar had a preventive effect on osteoporosis by decreasing osteoclastic activity. The hypothesis was tested in ovariectomized (OVX) rats and RANKL-differentiated osteoclast cells. OVX rats were given 0(OVX-CON), 0.5(OVX-MVL), 1(OVX-MVM), and 2(OVX-MVH) fermented mulberry vinegar (MV) mL/kg body weight (BW) daily for 12 weeks. Sham-operated rats had no MV supplementation (Normal-CON). The osteoporosis-related biomarkers were measured, and Micro-CT determined the bone mass of the femur. RANKL-differentiated Raw 264.7 cells were treated with MV (0–100 μg/mL). The cell viability, osteoporosis-related mRNA expression, and protein contents were measured. MV contained Acetobacter pasteurianus (7.31 log CFU/mL), citric acid (106 mg/mL), lactic acid (19.2 mg/mL), acetic acid (15.0 mg/mL), and rutin (0.36 mg/mL). OVX-MVM elevated the serum 17β-estradiol concentration similar to the Normal-CON group, but it did not prevent the decrease in uterine weight. OVX-MVM prevented the increase in osteoclastic-related parameters, including cathepsin K(CtsK), receptor activator of NF-κB ligand (RANKL), and tartrate-resistant acid phosphatase (TRAP) in the circulation. OVX-MVH also lowered C-telopeptide of type Ⅰ collagen as much as the Normal-CON group (p < 0.05). By contrast, OVX-MVH increased the serum osteoprotegerin concentration, an inhibitor of osteoclasts, better than the Normal-CON group (p < 0.05). These changes were integrated to alter the bone mineral density (BMD) in Micro-CT analysis: OVX-MVM and OVX-MVH prevented BMD decrease after OVX as much as the Normal-CON. In RANKL-differentiated osteoclast cells, the MV treatment for 24 and 48 h decreased RANKL-induced differentiation in osteoclast cells dose-dependently up to 100 µg/mL. Its decrease was related to inhibiting the TRAP activity and reducing TRAP-positive multinucleated cells during the five-day administration of RANKL. MV treatments also decreased mRNA expression of osteoclast-related genes (TRAP, Ctsk, OSCAR, and NFATc1). MV suppressed the protein contents of NFATc1 and c-FOS-related osteoclast. In conclusion, MV intake (1 mg/kg bw) protected against BMD loss mainly by inhibiting the osteoclastic activity (RANKL/RANK/TRAP) in OVX rats. MV may develop as a functional food for anti-osteoporosis in menopausal women.
Abundant Exact Travelling Wave Solutions for a Fractional Massive Thirring Model Using Extended Jacobi Elliptic Function Method
Authors: Shqair Mohammed , Alabedalhadi Mohammed , Al-Omari Shrideh , Al-Smadi Mohammed
Published: May 2022

The fractional massive Thirring model is a coupled system of nonlinear PDEs emerging in the study of the complex ultrashort pulse propagation analysis of nonlinear wave functions. This article considers the NFMT model in terms of a modified Riemann–Liouville fractional derivative. The novel travelling wave solutions of the considered model are investigated by employing an effective analytic approach based on a complex fractional transformation and Jacobi elliptic functions. The extended Jacobi elliptic function method is a systematic tool for restoring many of the well-known results of complex fractional systems by identifying suitable options for arbitrary elliptic functions. To understand the physical characteristics of NFMT, the 3D graphical representations of the obtained propagation wave solutions for some free physical parameters are randomly drawn for a different order of the fractional derivatives. The results indicate that the proposed method is reliable, simple, and powerful enough to handle more complicated nonlinear fractional partial differential equations in quantum mechanics.
Intimate Belonging—Intimate Becoming: How Police Officers and Migrant Gang Defectors Seek to (Re)shape Ties of Belonging in Denmark
Authors: Johansen Mette-Louise E.
Published: May 2022

This article examines the ways that Danish gang exit programs engage police officers and gang defectors in a pervasive work on belonging between gangs, kinship networks and the state. In urban Denmark, the majority of gang exit candidates are of ethnic-minority background and form part of the street-gang environment in marginalized migrant neighborhoods. This is an intimate social environment constituted by diasporic kinship networks, where gang formations are entangled with kinship formations. Hence, when gang defectors leave their gang, they also often leave their family and childhood home for a life in unfamiliar places and positions. As I show, gang desistance is thus a highly dilemmatic process in which gang defectors find themselves “unhinged” from meaningful social and kinship relationships and in search of new ways of embedding themselves into a social world. Based on an ethnographic study of gang exit processes in Denmark’s second largest city, Aarhus, this article shows how police officers and gang defectors seek to (re)shape ties of belonging between gangs, kinship networks and the state. The process, I argue, illuminates the intimate aspect of the notion of belonging, in which kin and state relatedness is deeply rooted in interpersonal spaces and relationships.
Exploring Relevant mRNAs and miRNAs in Injured Urethral Tissues of Rats with High-Throughput Sequencing
Authors: Lin Han , Guo Shiyong , Li Song , Shen Jihong , He Jianfeng , Zheng Yun , Gao Zhenhua
Published: May 2022

Acute urethral injuries caused by urethral endoscopy and other mechanical injuries are the main reasons for secondary infection and late urethral stricture. However, there are no studies to explore the transcriptomic changes in urethral injury and the molecular mechanism of urethral injury, which is important for the treatment and cure of urethral injury. Therefore, we used RNA-seq and sRNA-seq profiles from normal and injured urethral tissues to identify and characterize differentially expressed mRNAs and miRNAs. In total, we found 166 differentially expressed mRNAs, of which 69 were upregulated, and 97 were downregulated in injured urethral tissues. The differentially expressed mRNAs were mainly involved in the positive regulation of epithelial cell differentiation, focal adhesion, cell adhesion molecules, protein activation cascade, complement activation, complement and coagulation cascades, and chemokine-mediated signaling pathway. Additionally, we found six upregulated and four downregulated miRNAs, respectively, in the injured urethral tissues. Notably, their target genes were involved in the vascular endothelial growth factor receptor 2 binding, PI3k-Akt signaling pathway, and Notch signaling pathway. In summary, our results suggest that the cell damage response induced by mechanical injury activates the pathological immune response in a variety of ways in injured urethral tissues.
Development of a Highly Sensitive Loop-Mediated Isothermal Amplification Incorporated with Flocculation of Carbon Particles for Rapid On-Site Diagnosis of Blood Disease Bacterium Banana
Authors: Azizi Mohammad Malek Faizal , Lau Han Yih , Abu Bakar Norliza , Romeli Sohana , Mohd Yusof Muhammad Fairuz , Badrun Rafidah , Jaffar Nur Sulastri
Published: May 2022

Bananas are one of the most crucial fruit crops worldwide and significantly contribute to food security in developing countries. However, blood disease of bananas caused by Ralstonia syzygii subspecies celebensensis has become a threat to banana production. Rapid and accurate diagnosis of BDB for on-site detection is pivotal at an early stage for an effective disease control strategy. This study developed LAMP with specific primers targeting BDB, followed by a flocculation assay for visualising positive amplification in the LAMP assay. The assay was sensitive to picogram amounts of gDNA (0.5 pg). LAMP assay on BDB gDNA showed flocculation, but negative results on Fusarium oxysporus cubense and Ralstonia solanacearum confirming the specificity of the assays. Field testing conducted at MARDI headquarters and Taman Pertanian Universiti discovered that the LAMP-flocculation assays were successful in detecting BDB on symptomatic samples as well as on samples from a healthy plot with no symptom observed at the sampling stage, revealing that this assay can detect BDB at an early infection stage. The validation results showed that the LAMP-flocculation assay was comparable with the PCR technique. This newly developed technique is highly specific and sensitive for the early detection of BDB for the adoption of precautionary control measures.
The Impact of Implementing an Exergame Program on the Level of Reaction Time Optimization in Handball, Volleyball, and Basketball Players
Authors: Badau Dana , Badau Adela , Ene-Voiculescu Carmen , Larion Alin , Ene-Voiculescu Virgil , Mihaila Ion , Fleancu Julien Leonard , Tudor Virgil , Tifrea Corina , Cotovanu Adrian Sebastian , Abramiuc Alexandru
Published: May 2022

The main aim of the present study was to implement an exergame program that uses Fitlight technology to identify the impact on motor, recognition, and cognitive reaction times in junior athletes practicing team sports: basketball, handball, and volleyball. The second aim was to identifying differences in progress of the three types of reaction time between female and male players through computerized tests. The study included 360 subjects for basketball, 130 athletes of which were 68 male subjects and 62 female subjects; for handball, 124 athletes of which 64 were male subjects and 60 female athletes; for volleyball, 106 athletes of which 48 male were subjects and 48 female athletes. Characteristics of the experimental players: average age ± SD 13.60 ± 1.07; average sports experience ± SD 6.24 ± 0.92. The research included an initial and a final test between which a program of exergames was implemented over a period of 3 months focused on optimizing human reaction times. The evaluation of the reaction times was carried out through three computer games, the results being processed in SPSS 22. The relevant results of the research: for the simple motor reaction time (MSRT), the greatest progress between tests was the volleyball group, and for women, it was the basketball group; for the recognition reaction time (RRT), the male handball group and the female basketball group recorded the greatest progress; for the cognitive reactive time (CRT), the greatest progress was achieved by the male and female volleyball players. In all tests, the progress of the female basketball, handball, and volleyball players showed superior progress to similar male players. The results of the research highlighted the effectiveness of the experimental exergame program by using Fitlight technology in optimizing human reaction times in junior team-game athletes. Using computer games to evaluate reaction times allowed us to differentiate the evaluation on the types of human reactions under both standardized conditions but also under conditions of efficiency and attractiveness.
Evaluating Food Packaging Waste in Schools: A Systematic Literature Review
Authors: Heiges Jessica , Lee Danielle L. , Vollmer Laura , Wobbekind Kate , Thompson Hannah R. , Gosliner Wendi , Madsen Kristine A. , O’Neill Kate , Ritchie Lorrene D.
Published: May 2022

Public schools in the U.S. generate about 14,500 tons of municipal solid waste daily, and approximately 42% of that is food packaging generated by school foodservice, contributing significantly to the global packaging waste crisis. This literature review summarizes methods used to evaluate food packaging waste in school foodservice. This review has two objectives: first, to understand which methodologies currently exist to evaluate food packaging waste generation and disposal in school foodservice; and second, to describe the creation of and share a practical standardized instrument to evaluate food packaging waste generation and disposal in school foodservice. A systematic review was conducted using the following search terms: solid waste, school, cafeteria and food packaging, waste, and school. The final review included 24 studies conducted in school environments (kindergarten through twelfth grade or college/university), 16 of which took place in the U.S. Food packaging waste evaluations included objective methods of waste audits, models, and secondary data as well as subjective methods of qualitative observations, questionnaires, interviews, and focus groups. Large variation exists in the settings, participants, designs, and methodologies for evaluating school foodservice packaging waste. Lack of standardization was observed even within each methodology (e.g., waste audit). A new instrument is proposed to support comprehensive and replicable data collection, to further the understanding of school foodservice food packaging waste in the U.S., and to reduce environmental harms.
Applications and Current Medico-Legal Challenges of Telemedicine in Ophthalmology
Authors: Mazzuca Daniela , Borselli Massimiliano , Gratteri Santo , Zampogna Giovanna , Feola Alessandro , Della Corte Marcello , Guarna Francesca , Scorcia Vincenzo , Giannaccare Giuseppe
Published: May 2022

Background: The digital revolution is redesigning the healthcare model, and telemedicine offers a good example of the best cost-effectiveness ratio. The COVID-19 pandemic has catalysed the use of the telemedicine. The aim of this review is to describe and discuss the role and the main applications of telemedicine in the ophthalmic clinical practice as well as the related medico-legal aspects. Methods: 45 original articles and 5 reviews focused on this topic and published in English language from 1997 and 2021 were searched on the online databases of Pubmed, Scopus, Web of Sciences and Embase, by using the following key words: “telemedicine”, “privacy”, “ophthalmology”, “COVID-19” and “informed consent”. Results: Telemedicine is able to guarantee patient care using information and communication technologies. Technology creates an opportunity to link doctors with the aim of assessing clinical cases and maintaining high standards of care while performing and saving time as well. Ophthalmology is one of the fields in which telemedicine is most commonly used for patient management. Conclusions: Telemedicine offers benefits to patients in terms of saving time and costs and avoiding physical contact; however, it is necessary to point out significant limitations such as the absence of physical examinations, the possibility of transmission failure and potential violations of privacy and confidentiality.
The Effect of the Distance from a Path on Abiotic Conditions and Vascular Plant Species in the Undergrowth of Urban Forests and Parks
Authors: Kostrakiewicz-Gierałt Kinga , Gmyrek Katarzyna , Pliszko Artur
Published: May 2022

Urban forests and parks are essential for the maintenance of biodiversity as well as human health and well-being. Residents and tourists commonly use urban forests and parks for recreational and sport purposes, contributing to changes in vegetation. This study aimed to assess the effect of distance from formal paths on the abiotic conditions, vegetation cover, as well as ecological diversity of vascular plant species in the undergrowth of urban forests and parks. The investigations were carried out in 2021 in 10 urban forests and 10 urban parks located in Kraków (southern Poland), using a total of 400 plots (1 × 1 m) situated in close (CL) and further (FU) vicinity of formal paths. We found a positive effect of the distance from the path on the depth of the compact soil layer, vegetation cover and height of the tallest shoot in the undergrowth of urban forests and parks. On the other hand, the distance from the path had a negative effect on the number of vascular plant species in the undergrowth in both forests and parks. Forests and parks differed significantly from each other in light intensity, the content of P in soil, depth of compact soil layer, number of species, as well as in cover-abundance of species representing different life forms, dispersal types, habitat affiliations and origins. Trampling leads to low plant cover and height of the undergrowth, as well as contributing to shallow localization of the compact soil layer near paths. Human movement on paths (walking, running, biking) with accompanying pets contributes to the successful dispersal of plants, resulting in high species richness. High light intensity in urban parks enhances the total number of species, cover-abundance of meadow and grassland plants, as well as cover-abundance of hemicryptophytes. The number of alien species was higher in parks than in forests, but the cover-abundance of alien plants was higher in forests than in parks. Urban forests are more suitable for the growth and biomass production of some alien herbs than urban parks, as mowing commonly used in parks appears to be an important factor in reducing their cover abundance. Regular fertilization and irrigation contribute to the high content of phosphorus in the soil, as well as to the high cover-abundance of meadow and grassland plants in urban parks. Urban forests enhance cover abundance of plants with dispersal mechanisms of the Bidens and Lycopodium types, whereas urban parks promote cover abundance of plants with the dispersal of the Allium type. Further study is needed to confirm the role of urban forests and parks in the preservation of ancient forest species, as well as to develop an appropriate design of paths that will allow the protection of vegetation and soil in urban forests and parks.
Hydration Status in Men Working in Different Thermal Environments: A Pilot Study
Authors: Orysiak Joanna , Młynarczyk Magdalena , Tomaszewski Paweł
Published: May 2022

The aim of this study was to determine the effects of different seasons of the year and the time of day (before work vs. after work) on hydration status in men. The study involved sixty foresters who spent most of the work outdoors. During three seasons of the year (summer, autumn, and winter), indices of hydration status (body mass (BM) and percentage change of BM, total body water (TBW) and percentage change of TBW, serum osmolality (Sosm) and percentage change of Sosm, urine osmolality, urine-specific gravity (USG), urine color, and thirst) were determined before work on the first day (time point 1 used as baseline), immediately after work on the first day (time point 2), and before work on the following day (time point 3). USG decreased at time point 2 compared to time point 1 (p < 0.001) and time point 3 (p = 0.03). At time point 2 (p = 0.002) in winter and time point 3 in autumn (p = 0.049), serum osmolality was higher than in summer. In conclusion, the differences in hydration status depended on the time of day and season. A large percentage of foresters come to work inadequately hydrated, especially in colder seasons compared to summer.
A Person-Centered Approach to Moralization—The Case of Vaping
Authors: Arhiri Laura , Gherman Mihaela A. , Holman Andrei C.
Published: May 2022

Using e-cigarettes for smoking cessation is a controversial topic among health experts. Evidence suggests that vaping might have been moralized among the general public. Despite the detrimental consequences of moralizing health behaviors on social cohesion and health, some argue for using moralization strategically to prevent and combat vaping. We aim to add to the body of literature showing the dangers of moralization in health by proposing a person-centered approach to the moralization of anti-vaping attitudes. Our cross-sectional survey explores the moralization of anti-vaping attitudes and its predictors on a convenience sample of 348 Romanian never-vapers, before the final vote to severely restrict vaping. By fitting a hierarchical regression model on our data, we found support for a unique contribution of negative prototypes (β = 0.13) and opinions of vapers (β = 0.08) in predicting moralization, with significant contributions of piggybacking on moralized self-control, on moralized attitudes toward smoking and on sanctity/degradation, disgust, anger, harm to children, and gender. Together, these variables explained 56% of the variance of the moralization of anti-vaping attitudes. Our findings add to our knowledge of motivated moralization and advise against using moralization in health, suggesting that people may weaponize it to legitimize group dislike.
The Impact of an After-School Physical Activity Program on Children’s Physical Activity and Well-Being during the COVID-19 Pandemic: A Mixed-Methods Evaluation Study
Authors: Caldwell Hilary A. T. , Miller Matthew B. , Tweedie Constance , Zahavich Jeffery B. L. , Cockett Ella , Rehman Laurene
Published: May 2022

Introduction: This study evaluated the impact of the Build Our Kids’ Success (BOKS) after-school program on children’s physical activity (PA) and well-being during the COVID-19 pandemic. Methods: Program leaders, children, and their parents were recruited from after-school programs in Nova Scotia, Canada, that delivered BOKS programming in Fall 2020. After participating, Grade 4–6 children (n = 14) completed the Physical Literacy Assessment for Youth Self (PLAYself), Physical Activity Questionnaire for Older Children (PAQ-C), the Physical Activity Enjoyment Scale (PACES), and 5 National Institutes of Health (NIH) Patient-Reported Outcomes Measures Information System (PROMIS) scales. Children (n = 7), parents (n = 5), and program leaders (n = 3) completed interviews, which were analyzed for themes inductively. Results: The average PAQ-C score was 2.70 ± 0.48, PLAYself was 68.23 ± 13.12, and PACES was 4.22 ± 0.59 (mean ± SD). NIH PROMIS scores were below standard means (cognitive function, family relationships) or within normal limits (peer relationships, positive affect, and life satisfaction). A thematic analysis of interviews revealed that children’s PA levels were impacted by the pandemic and that BOKS positively impacted children’s physical well-being and integrated well with school-based activities. Conclusions: Participation in BOKS provided an overall positive experience and may have mitigated COVID-19-related declines in PA in well-being. The results of this evaluation can inform future physically-active after-school programming.
New Insight on the In Vitro Effects of Melatonin in Preserving Human Sperm Quality
Authors: Minucci Sergio , Venditti Massimo
Published: May 2022

Spermatozoa (SPZ) are sensitive to stressful conditions, particularly oxidative stress, which alters their quality; thus, the use of protective molecules as an antioxidant is encouraged. Herein, we used melatonin (MLT) to investigate its in vitro effects on human sperm parameters under conditions of oxidative stress induced by cadmium (Cd). Fifteen human semen samples were divided into control, Cd-treated, MLT-treated, and Cd+MLT-treated groups and analyzed after 30 min, 6 h, and 24 h of exposure. Results showed a time-dependent decrease in SPZ motility, DNA integrity, and increased apoptosis induced by oxidative stress, and these effects were counteracted by MLT co-treatment. Based on these data, we further explored additional parameters just at 24 h. The induced oxidative stress, highlighted by the increased lipid peroxidation, reduced the percentage of SPZ able to undertake acrosome reaction and altered the levels and localization of some protein markers of motility (PREP, RSPH6A), morphology (DAAM1), and acrosome membrane (PTMA, IAM38); all these effects were counteracted by MLT co-treatment. Interestingly, MLT alone was able to ameliorate motility at 30 min of incubation compared to the control, while at 24 h, it prevented the physiological alteration in terms of motility, DNA integrity, and apoptosis. Collectively, the data encourage MLT use as an integrative molecule to ameliorate human gamete quality when compromised by stressful conditions.
MicroRNA-449a Inhibits Triple Negative Breast Cancer by Disturbing DNA Repair and Chromatid Separation
Authors: Vajen Beate , Bhowmick Rahul , Greiwe Luisa , Schäffer Vera , Eilers Marlies , Reinkens Thea , Stalke Amelie , Schmidt Gunnar , Fiedler Jan , Thum Thomas , DeLuca David S. , Hickson Ian D. , Schlegelberger Brigitte , Illig Thomas , Skawran Britta
Published: May 2022

Chromosomal instability (CIN) can be a driver of tumorigenesis but is also a promising therapeutic target for cancer associated with poor prognosis such as triple negative breast cancer (TNBC). The treatment of TNBC cells with defects in DNA repair genes with poly(ADP-ribose) polymerase inhibitor (PARPi) massively increases CIN, resulting in apoptosis. Here, we identified a previously unknown role of microRNA-449a in CIN. The transfection of TNBC cell lines HCC38, HCC1937 and HCC1395 with microRNA-449a mimics led to induced apoptosis, reduced cell proliferation, and reduced expression of genes in homology directed repair (HDR) in microarray analyses. EME1 was identified as a new target gene by immunoprecipitation and luciferase assays. The reduced expression of EME1 led to an increased frequency of ultrafine bridges, 53BP1 foci, and micronuclei. The induced expression of microRNA-449a elevated CIN beyond tolerable levels and induced apoptosis in TNBC cell lines by two different mechanisms: (I) promoting chromatid mis-segregation by targeting endonuclease EME1 and (II) inhibiting HDR by downregulating key players of the HDR network such as E2F3, BIRC5, BRCA2 and RAD51. The ectopic expression of microRNA-449a enhanced the toxic effect of PARPi in cells with pathogenic germline BRCA1 variants. The newly identified role makes microRNA-449a an interesting therapeutic target for TNBC.
Application of Multivariate Adaptive Regression Splines (MARSplines) for Predicting Antitumor Activity of Anthrapyrazole Derivatives
Authors: Gackowski Marcin , Szewczyk-Golec Karolina , Pluskota Robert , Koba Marcin , Mądra-Gackowska Katarzyna , Woźniak Alina
Published: May 2022

An approach using multivariate adaptive regression splines (MARSplines) was applied for quantitative structure–activity relationship studies of the antitumor activity of anthrapyrazoles. At the first stage, the structures of anthrapyrazole derivatives were subjected to geometrical optimization by the AM1 method using the Polak–Ribiere algorithm. In the next step, a data set of 73 compounds was coded over 2500 calculated molecular descriptors. It was shown that fourteen independent variables appearing in the statistically significant MARS model (i.e., descriptors belonging to 3D-MoRSE, 2D autocorrelations, GETAWAY, burden eigenvalues and RDF descriptors), significantly affect the antitumor activity of anthrapyrazole compounds. The study confirmed the benefit of using a modern machine learning algorithm, since the high predictive power of the obtained model had proven to be useful for the prediction of antitumor activity against murine leukemia L1210. It could certainly be considered as a tool for predicting activity against other cancer cell lines.
The Emerging Role of Extracellular Vesicles Detected in Different Biological Fluids in COPD
Authors: Neri Tommaso , Celi Alessandro , Tinè Mariaenrica , Bernardinello Nicol , Cosio Manuel G. , Saetta Marina , Nieri Dario , Bazzan Erica
Published: May 2022

The pathogenesis of chronic obstructive pulmonary disease (COPD) is characterized by complex cellular and molecular mechanisms, not fully elucidated so far. It involves inflammatory cells (monocytes/macrophages, neutrophils, lymphocytes), cytokines, chemokines and, probably, new players yet to be clearly identified and described. Chronic local and systemic inflammation, lung aging and cellular senescence are key pathological events in COPD development and progression over time. Extracellular vesicles (EVs), released by virtually all cells both as microvesicles and exosomes into different biological fluids, are involved in intercellular communication and, therefore, represent intriguing players in pathobiological mechanisms (including those characterizing aging and chronic diseases); moreover, the role of EVs as biomarkers in different diseases, including COPD, is rapidly gaining recognition. In this review, after recalling the essential steps of COPD pathogenesis, we summarize the current evidence on the roles of EVs collected in different biological mediums as biomarkers in COPD and as potential players in the specific mechanisms leading to disease development. We will also briefly review the data on EV as potential therapeutic targets and potential therapeutic agents.
Purified Clinoptilolite-Tuff as an Efficient Sorbent for Gluten Derived from Food
Authors: Ranftler Carmen , Röhrich Andreas , Sparer Andreas , Tschegg Cornelius , Nagl Dietmar
Published: May 2022

Various gluten-related diseases (celiac disease, wheat allergy, gluten sensitivity) are known and their incidence is growing. Gluten is a specific type of plant storage protein that can impair the health of gluten-prone persons following consumption, depending on the origin. The most severe effects are induced by wheat, barley, and rye. The only treatment is based on the absolute avoidance of those foods, as even traces might have severe effects on human well-being. With the goal of binding gluten impurities after ingestion, an in vitro setting was created. A special processed kind of zeolite, purified clinoptilolite-tuff (PCT), was implemented as an adsorber of gluten derived from different origins. Zeolites are known for their excellent sorption capacities and their applications in humans and animals have been studied for a long time. Tests were also performed in artificial gastric and intestinal fluids, and the adsorption capacity was determined via a certified validated method (ELISA). Depending on the kind of gluten source, 80–130 µg/mg of gluten were bound onto PCT. Hence, purified clinoptilolite-tuff, which was successfully tested for wheat, barley, and rye, proved to be suitable for the adsorption of gluten originating from different kinds of crops. This result might form the basis for an expedient human study in the future.
Nutritional Management of Thyroiditis of Hashimoto
Authors: Danailova Yana , Velikova Tsvetelina , Nikolaev Georgi , Mitova Zorka , Shinkov Alexander , Gagov Hristo , Konakchieva Rossitza
Published: May 2022

Since the thyroid gland is one of the organs most affected by autoimmune processes, many patients with thyroiditis of Hashimoto (TH) seek medical advice on lifestyle variance and dietary modifications to improve and maintain their hyroid function. In this review, we aim to present and discuss some challenges associated with the nutritional management of TH, focusing on environmental and dietary deficits, inflammatory and toxic nutrients, cyanotoxins, etc. We discuss the relationships among different diets, chronic inflammation, and microbiota, and their impact on the development and exacerbation of TH in detail. We share some novel insights into the role of vitamin D and melatonin for preserving thyroid function during chronic inflammation in autoimmune predisposed subjects. A comprehensive overview is provided on anti-inflammatory nutrients and ecological diets, including foods for cleansing and detoxification, which represent strategies to prevent relapses and achieve overall improvement of life quality. In conclusion, data from biomedical and clinical studies provide evidence that an appropriate dietary and lighting regimen could significantly improve the function of the thyroid gland and reduce the reactivity of autoantibodies in TH. Compliance with nutritional guidelines may help TH patients to reduce the need for medicines.
Clinical Parameters and Epigenetic Biomarkers of Plaque Vulnerability in Patients with Carotid Stenosis
Authors: Carballo-Perich Laia , Puigoriol-Illamola Dolors , Bashir Saima , Terceño Mikel , Silva Yolanda , Gubern-Mérida Carme , Serena Joaquín
Published: May 2022

Atheromatous disease is the first cause of death and dependency in developed countries and carotid artery atherosclerosis is one of the main causes of severe ischaemic strokes. Current management strategies are mainly based on the degree of stenosis and patient selection has limited accuracy. This information could be complemented by the identification of biomarkers of plaque vulnerability, which would permit patients at greater and lesser risk of stroke to be distinguished, thus enabling a better selection of patients for surgical or intensive medical treatment. Although several circulating protein-based biomarkers with significance for both the diagnosis of carotid artery disease and its prognosis have been identified, at present, none have been clinically implemented. This review focuses especially on the most relevant clinical parameters to take into account in routine clinical practice and summarises the most up-to-date data on epigenetic biomarkers of carotid atherosclerosis and plaque vulnerability.
RNase 7 Inhibits Uropathogenic Escherichia coli-Induced Inflammation in Bladder Cells under a High-Glucose Environment by Regulating the JAK/STAT Signaling Pathway
Authors: Ho Chen-Hsun , Liao Pin-Wen , Fan Chia-Kwung , Liu Shih-Ping , Cheng Po-Ching
Published: May 2022

Antimicrobial peptides (AMPs), which are natural antibiotics, protect against pathogens invading the urinary tract. RNase 7 with antimicrobial properties has rapid and powerful suppressive effects against Gram-positive and Gram-negative bacterial infections. However, its detailed antibacterial mechanisms have not been fully determined. Here, we investigate whether RNase 7 had an impact on bladder cells under uropathogenic Escherichia coli (UPEC) infection in a high-glucose environment using in vitro GFP-UPEC-infected bladder cell and PE-labeled TLR4, STAT1, and STAT3 models. We provide evidence of the suppressive effects of RNase 7 on UPEC infection and UPEC-induced inflammatory responses by regulating the JAK/STAT signaling pathway using JAK inhibitor and STAT inhibitor blocking experiments. Pretreatment with different concentrations of RNase 7 for 24 h concentration-dependently suppressed UPEC invasion in bladder cells (5 μg/mL reducing 45%; 25 μg/mL reducing 60%). The expressions of TLR4, STAT1, and STAT3 were also downregulated in a concentration-dependent manner after RNase 7 pretreatment (5 μg/mL reducing 35%, 54% and 35%; 25 μg/mL reducing 60%, 75% and 64%, respectively). RNase 7-induced decrease in UPEC infection in a high-glucose environment not only downregulated the expression of TLR4 protein and the JAK/STAT signaling pathway but also decreased UPEC-induced secretion of exogenous inflammatory IL-6 and IL-8 cytokines, although IL-8 levels increased in the 25 μg/mL RNase 7-treated group. Thus, inhibition of STAT affected pSTAT1, pSTAT3, and TLR4 expression, as well as proinflammatory IL-6 and IFN-γ expression. Notably, blocking JAK resulted in the rebound expression of related proteins, especially pSTAT1, TLR4, and IL-6. The present study showed the suppressive effects of RNase 7 on UPEC infection and induced inflammation in bladder epithelial cells in a high-glucose environment. RNase 7 may be an anti-inflammatory and anti-infective mediator in bladder cells by downregulating the JAK/STAT signaling pathway and may be beneficial in treating cystitis in DM patients. These results will help clarify the correlation between AMP production and UTI, identify the relationship between urinary tract infection and diabetes in UTI patients, and develop novel diagnostics or possible treatments targeting RNase 7.
Close Related Drug-Resistance Beijing Isolates of Mycobacterium tuberculosis Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model
Authors: Cerezo-Cortés María Irene , Rodríguez-Castillo Juan Germán , Mata-Espinosa Dulce Adriana , Bini Estela Isabel , Barrios-Payan Jorge , Zatarain-Barrón Zyanya Lucia , Anzola Juan Manuel , Cornejo-Granados Fernanda , Ochoa-Leyva Adrian , Del Portillo Patricia , Murcia Martha Isabel , Hernández-Pando Rogelio
Published: May 2022

Mycobacterium tuberculosis (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-resistant cluster and shows a fatal outcome in patients. To better understand virulence determinants, we performed a transcriptomic analysis with a Beijing-Like SIT-190 isolate (BL-323), and Beijing-Classic SIT-1 isolate (BC-391) in progressive tuberculosis (TB) murine model. Bacterial RNA was extracted from mice lungs on days 3, 14, 28, and 60. On average, 0.6% of the total reads mapped against MTB genomes and of those, 90% against coding genes. The strains were independently associated as determined by hierarchical cluster and multidimensional scaling analysis. Gene ontology showed that in strain BL-323 enriched functions were related to host immune response and hypoxia, while proteolysis and protein folding were enriched in the BC-391 strain. Altogether, our results suggested a differential bacterial transcriptional program when evaluating these two closely related strains. The data presented here could potentially impact the control of this emerging, highly virulent, and drug-resistant genotype.
Relationship between Telomere Length, TERT Genetic Variability and TERT, TP53, SP1, MYC Gene Co-Expression in the Clinicopathological Profile of Breast Cancer
Authors: Dratwa Marta , Wysoczanska Barbara , Brankiewicz Wioletta , Stachowicz-Suhs Martyna , Wietrzyk Joanna , Matkowski Rafał , Ekiert Marcin , Szelachowska Jolanta , Maciejczyk Adam , Szajewski Mariusz , Baginski Maciej , Bogunia-Kubik Katarzyna
Published: May 2022

The molecular mechanisms of telomerase reverse transcriptase (TERT) upregulation in breast cancer (BC) are complex. We compared genetic variability within TERT and telomere length with the clinical data of patients with BC. Additionally, we assessed the expression of the TERT, MYC, TP53 and SP1 genes in BC patients and in BC organoids (3D cell cultures obtained from breast cancer tissues). We observed the same correlation in the blood of BC patients and in BC organoids between the expression of TERT and TP53. Only in BC patients was a correlation found between the expression of the TERT and MYC genes and between TP53 and MYC. We found associations between TERT genotypes (rs2735940 and rs10069690) and TP53 expression and telomere length. BC patients with the TT genotype rs2735940 have a shorter telomere length, but patients with A allele rs10069690 have a longer telomere length. BC patients with a short allele VNTR-MNS16A showed higher expression of the SP1 and had a longer telomere. Our results bring new insight into the regulation of TERT, MYC, TP53 and SP1 gene expression related to TERT genetic variability and telomere length. Our study also showed for the first time a similar relationship in the expression of the above genes in BC patients and in BC organoids. These findings suggest that TERT genetic variability, expression and telomere length might be useful biomarkers for BC, but their prognostic value may vary depending on the clinical parameters of BC patients and tumor aggressiveness.
Revealing the Immune Heterogeneity between Systemic Lupus Erythematosus and Rheumatoid Arthritis Based on Multi-Omics Data Analysis
Authors: Zhang Yuntian , Lee Tzong-Yi
Published: May 2022

The pathogenesis of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) are greatly influenced by different immune cells. Nowadays both T-cell receptor (TCR) and B-cell receptor (BCR) sequencing technology have emerged with the maturity of NGS technology. However, both SLE and RA peripheral blood TCR or BCR repertoire sequencing remains lacking because repertoire sequencing is an expensive assay and consumes valuable tissue samples. This study used computational methods TRUST4 to construct TCR repertoire and BCR repertoire from bulk RNA-seq data of both SLE and RA patients’ peripheral blood and analyzed the clonality and diversity of the immune repertoire between the two diseases. Although the functions of immune cells have been studied, the mechanism is still complicated. Differentially expressed genes in each immune cell type and cell–cell interactions between immune cell clusters have not been covered. In this work, we clustered eight immune cell subsets from original scRNA-seq data and disentangled the characteristic alterations of cell subset proportion under both SLE and RA conditions. The cell–cell communication analysis tool CellChat was also utilized to analyze the influence of MIF family and GALECTIN family cytokines, which were reported to regulate SLE and RA, respectively. Our findings correspond to previous findings that MIF increases in the serum of SLE patients. This work proved that the presence of LGALS9, PTPRC and CD44 in platelets could serve as a clinical indicator of rheumatoid arthritis. Our findings comprehensively illustrate dynamic alterations in immune cells during pathogenesis of SLE and RA. This work identified specific V genes and J genes in TCR and BCR that could be used to expand our understanding of SLE and RA. These findings provide a new insight inti the diagnosis and treatment of the two autoimmune diseases.
Frailty as a Predictor of In-Hospital Outcome in Patients with Myocardial Infarction
Authors: Węgiel Michał , Kleczyński Paweł , Dziewierz Artur , Rzeszutko Łukasz , Surdacki Andrzej , Bartuś Stanisław , Rakowski Tomasz
Published: May 2022

(1) Background: Frailty is a condition associated with aging, co-morbidity, and disability. We aimed to investigate the relationship between frailty and in-hospital outcome in patients with myocardial infarction (MI), including the occurrence of delirium, hospital-acquired pneumonia (HAP), and length of hospital stay. (2) Methods: We analyzed 55 patients ≥ 75 years old with ST-elevation and non-ST-elevation MI. Assessment with Abbreviated Mental Test Score (AMTS), Activity of Daily Living (ADL), Instrumental Activity of Daily Living (IADL) and Clinical Frailty Scale (CFS) was performed. (3) Results: In ROC analysis, IADL and CFS presented good predictive values for the occurrence of delirium (AUC = 0.81, p = 0.023, and AUC = 0.86, p = 0.009, respectively). For predicting HAP, only AMTS showed a significant value (AUC = 0.69, p = 0.036). In regression analyses, all tests presented significant predictive values for delirium. For predicting HAP, only IADL and CFS presented significant values (in an analysis adjusted for age, gender and type of MI). Frail patients (≥5 points in CFS) had longer hospital stays (10 days IQR: 8–17 vs. 8 days IQR: 7–10; p = 0.03). (4) Conclusions: While recognizing the limitations of our study associated with the relatively low sample size, we believe that our analysis shows that frailty is a predictor of poorer in-hospital outcomes in patients with MI, including higher rates of delirium, HAP and longer hospital stay.
The Cardiovascular-Mortality-Based Estimate for Normal Range of the Ankle–Brachial Index (ABI)
Authors: Peltonen Essi , Laivuori Mirjami , Vakhitov Damir , Korhonen Päivi , Venermo Maarit , Hakovirta Harri
Published: May 2022

Background: The ankle–brachial index (ABI) is a first-line examination in cardiovascular risk evaluation. Since cut-off values for normal ABI vary, the aim of the present study was to identify the cardiovascular-mortality-based estimate for the normal range of the ABI. After determining the reference range for the ABI, the corresponding toe–brachial index (TBI) and toe pressure for normal ABI were analyzed. Methods: All consecutive non-invasive pressure measurements in the vascular laboratory of a large university hospital 2011–2013 inclusive were collected and combined with patient characteristics and official dates and causes of death. Patients with an ABI range of 0.8–1.4 on both lower limbs were included in this study. Results: From 2751 patients, 868 had bilateral ABI values within the inclusion. Both ABI category ranges 0.80–0.89 and 0.90–0.99 had poorer survival compared to ABI categories 1.00–1.29 (p < 0.05). The 1-, 3-, and 5-year cardiovascular-death-free survival for respective ABI categories 0.80–0.99 vs. 1.00–1.29 were 90% vs. 96%, 84% vs. 92%, and 60% vs. 87%. The 1-, 3-, and 5-year overall survival for ABI categories 0.80–0.99 vs. 1.00–1.29 were 85% vs. 92%, 75% vs. 83%, and 42% vs. 74%. Conclusions: Borderline ABI (0.90–0.99) associates with higher overall and cardiovascular mortality compared to ABI values 1.00–1.29.