Clinical and Biochemical Characteristics of Patients with Various Clinical Manifestations of Gastroesophageal Reflux Disease
Authors:   Scientific and Research Institute of Medical Problems of the North of the Federal Research Centre “Krasnoyarsk Scientific Centre of the Siberian Branch of the Russian Academy of Science”, Tsukanov V.V. ,   Krasnoyarsk, Russian Federation, Tonkikh Yu.L. ,   Scientific and Research Institute of Medical Problems of the North of the Federal Research Centre “Krasnoyarsk Scientific Centre of the Siberian Branch of the Russian Academy of Science”; Krasnoyarsk, Russian Federation, Kasparov E.V. ,   Scientific and Research Institute of Medical Problems of the North of the Federal Research Centre “Krasnoyarsk Scientific Centre of the Siberian Branch of the Russian Academy of Science”; Krasnoyarsk, Russian Federation, Vasyutin A.V. ,   Scientific and Research Institute of Medical Problems of the North of the Federal Research Centre “Krasnoyarsk Scientific Centre of the Siberian Branch of the Russian Academy of Science”; Krasnoyarsk, Russian Federation, Peretyatko O.V. ,   Scientific and Research Institute of Medical Problems of the North of the Federal Research Centre “Krasnoyarsk Scientific Centre of the Siberian Branch of the Russian Academy of Science”; Krasnoyarsk, Russian Federation
Published: January 2022

Study Objective: To study the clinical manifestations and oxidative stress parameters in patients with various variants of gastroesophageal reflux disease (GERD). Study Design: Cross-sectional comparative study. Materials and Methods. We examined 119 patients with various clinical variants of GERD: non-erosive reflux disease (n = 42); stage A-B erosive esophagitis (n = 27); stage C-D erosive esophagitis (n = 24), and Barrett esophagus (n = 26). All patients underwent magnified and narrow-band esophagus endoscopy with biopsy. Enzyme-linked immunosorbent assay was used to measure malondialdehyde (MDA), catalase and superoxide dismutase (SOD) concentration in biopsy samples and blood of all patients. Esophagus biopsy samples were examined histochemically in 29 patients with suspected Barrett esophagus. Study Results. Only patients with stage C-D esophagitis and Barrett esophagus complained of dysphagia and odynophagia. Epigastric burning (at least 3 times a week) was recorded twice more often in patients with stage C-D esophagitis and Barrett esophagus compared to patients with non-erosive reflux disease and stage A-B esophagitis. In patients with stage C-D esophagitis and Barrett esophagus, MDA concentration in esophagus biopsy samples was 2.2–3.6 times higher (p < 0.001), whereas SOD and catalase levels were 1.5 times higher (p < 0.01 and p < 0.03, respectively) vs patients with non-erosive reflux disease and stage A-B esophagitis. Blood serum demonstrated similar, but less marked changes. Conclusion. We have found out a more marked clinical manifestation in patients with stage C-D esophagitis and Barrett esophagus. The demonstrated increase in MDA level in esophagus biopsy samples and blood of patients with stage C-D esophagitis and Barrett esophagus makes it possible to assume that the oxidative stress is a marker of unfavourable course of GERD. Keywords: gastroesophageal reflux disease, Barrett esophagus, lipid peroxidation, antioxidant protection, oxidative stress.