bluuebunny/crossref_metadata_2025 huggingface dataset:
Auto-immunoproteomics analysis of COVID-19 ICU patients revealed increased levels of autoantibodies related to the male reproductive system
Authors: Schmidt Frank , Abdesselem Houari B. , Suhre Karsten , Vaikath Nishant N. , Sohail Muhammad U. , Al-Nesf Maryam , Bensmail Ilham , Mashod Fathima , Sarwath Hina , Bernhardt Joerg , Schaefer-Ramadan Stephanie , Tan Ti-Myen , Morris Priscilla E. , Schenck Edward J. , Price David , Mohamed-Ali Vidya , Al-Maadheed Mohammed , Arredouani Abdelilah , Decock Julie , Blackburn Jonathan M. , Choi Augustine M. K. , El-Agnaf Omar M.
Published: July 2023

Background: Coronavirus disease (COVID-19) manifests many clinical symptoms, including an exacerbated immune response and cytokine storm. Autoantibodies in COVID-19 may have severe prodromal effects that are poorly understood. The interaction between these autoantibodies and self-antigens can result in systemic inflammation and organ dysfunction. However, the role of autoantibodies in COVID-19 complications has yet to be fully understood.

Methods: The current investigation screened two independent cohorts of 97 COVID-19 patients [discovery (Disc) cohort from Qatar (case = 49 vs. control = 48) and replication (Rep) cohort from New York (case = 48 vs. control = 28)] utilizing high-throughput KoRectly Expressed (KREX) Immunome protein-array technology. Total IgG autoantibody responses were evaluated against 1,318 correctly folded and full-length human proteins. Samples were randomly applied on the precoated microarray slides for 2 h. Cy3-labeled secondary antibodies were used to detect IgG autoantibody response. Slides were scanned at a fixed gain setting using the Agilent fluorescence microarray scanner, generating a 16-bit TIFF file. Group comparisons were performed using a linear model and Fisher’s exact test. Differentially expressed proteins were used for KEGG and WIKIpathway annotation to determine pathways in which the proteins of interest were significantly over-represented.

Results and conclusion: Autoantibody responses to 57 proteins were significantly altered in the COVID-19 Disc cohort compared to healthy controls (p ≤ 0.05). The Rep cohort had altered autoantibody responses against 26 proteins compared to non-COVID-19 ICU patients who served as controls. Both cohorts showed substantial similarities (r2 = 0.73) and exhibited higher autoantibody responses to numerous transcription factors, immunomodulatory proteins, and human disease markers. Analysis of the combined cohorts revealed elevated autoantibody responses against SPANXN4, STK25, ATF4, PRKD2, and CHMP3 proteins in COVID-19 patients. The sequences for SPANXN4 and STK25 were cross-validated using sequence alignment tools. ELISA and Western blot further verified the autoantigen–autoantibody response of SPANXN4. SPANXN4 is essential for spermiogenesis and male fertility, which may predict a potential role for this protein in COVID-19-associated male reproductive tract complications, and warrants further research.
Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications
Authors: Farinati Silvia , Draga Samela , Betto Angelo , Palumbo Fabio , Vannozzi Alessandro , Lucchin Margherita , Barcaccia Gianni
Published: July 2023

Plant male sterility (MS) represents the inability of the plant to generate functional anthers, pollen, or male gametes. Developing MS lines represents one of the most important challenges in plant breeding programs, since the establishment of MS lines is a major goal in F1 hybrid production. For these reasons, MS lines have been developed in several species of economic interest, particularly in horticultural crops and ornamental plants. Over the years, MS has been accomplished through many different techniques ranging from approaches based on cross-mediated conventional breeding methods, to advanced devices based on knowledge of genetics and genomics to the most advanced molecular technologies based on genome editing (GE). GE methods, in particular gene knockout mediated by CRISPR/Cas-related tools, have resulted in flexible and successful strategic ideas used to alter the function of key genes, regulating numerous biological processes including MS. These precision breeding technologies are less time-consuming and can accelerate the creation of new genetic variability with the accumulation of favorable alleles, able to dramatically change the biological process and resulting in a potential efficiency of cultivar development bypassing sexual crosses. The main goal of this manuscript is to provide a general overview of insights and advances into plant male sterility, focusing the attention on the recent new breeding GE-based applications capable of inducing MS by targeting specific nuclear genic loci. A summary of the mechanisms underlying the recent CRISPR technology and relative success applications are described for the main crop and ornamental species. The future challenges and new potential applications of CRISPR/Cas systems in MS mutant production and other potential opportunities will be discussed, as generating CRISPR-edited DNA-free by transient transformation system and transgenerational gene editing for introducing desirable alleles and for precision breeding strategies.
Young para-athletes display more hedonic well-being than people with disabilities not taking part in competitive sports: insights from a multi-country survey
Authors: Puce Luca , Biz Carlo , Cerchiaro Mariachiara , Scapinello Davide , Giarrizzo Luca , Trompetto Carlo , Marinelli Lucio , Trabelsi Khaled , Samanipour Mohammad Hossein , Bragazzi Nicola Luigi , Ruggieri Pietro
Published: July 2023

Hedonic well-being relates to how individuals experience and rate their lives. People with disabilities due to their pathology may more frequently suffer from anxiety and depressive disorders than their able-bodied counterparts. Sports participation is an essential way to cope with disability. On the other hand, compared with their able-bodied peers, para-athletes undergo a unique series of stressors. Little is known in terms of hedonic well-being in this specific population. We present the results of a multi-country survey of self-perceived hedonic well-being by para-athletes of different sports disciplines and a control group (disabled individuals not playing competitive sports), using the “Psychological General Well-Being Index” (PGWBI). We included 1,208 participants, aged 17.39 years, 58.4% male, 41.6% female, and 70.3% para-athletes. Para-athletes exhibited higher well-being than disabled people, for all domains of the PGWBI scale. The nature of disability/impairment was significant, with those with acquired disability reporting lower well-being. Those taking part in wheelchair basketball, para-athletics, and para-swimming competitions had a higher likelihood of reporting well-being, whereas those engaged in wheelchair rugby exhibited lower well-being compared with controls. This large-scale investigation can enable a better understanding of the self-perceived hedonic well-being of disabled people.
Factor structure of the patient health questionnaire-4 in adults with attention-deficit/hyperactivity disorder
Authors: Havnen Audun , Lydersen Stian , Mandahl Arthur , Lara-Cabrera Mariela Loreto
Published: July 2023

Background

Persons with attention-deficit/hyperactivity disorder (ADHD) frequently experience symptoms of anxiety and depression. In this population, there is a need for validated brief self-report screening questionnaires to assess the severity of comorbid mental health problems. The Patient Health Questionnaire 4 (PHQ-4) is a self-report questionnaire that may contribute to this purpose as it can screen for both disorders efficiently. However, this will be the first study examining the factor structure of the PHQ-4 in samples of adults with ADHD, and also evaluating the validity of the Norwegian version of the PHQ-4.

Objectives

The aim of the current cross-sectional study was to examine the factor structure and validity of the Norwegian version of the PHQ-4 in a sample of adults who reported having been diagnosed with ADHD.

Methods

Of 496 invited, a total of 326 participants (66%) completed the PHQ-4, The World Health Organization Five Well-Being Index, the Oslo Social Support Scale and the 4-item Perceived Stress Scale electronically in a web-portal between the 9th and 30th of June 2020.

Results

Confirmatory factor analysis of the PHQ-4 supported a two-factor structure [RMSEA = 0.038 (90% CI 0.000–0.159), CFI = 1.00, TLI = 0.999, SRMR = 0.004], consisting of a depression factor and an anxiety factor. Standardized factor loadings were 0.79 to 0.97. The PHQ-4 was negatively correlated with well-being and social support and positively correlated with perceived level of stress.

Conclusion

This study indicates promising psychometric properties of the PHQ-4 as a measure of anxiety and depressive symptoms in adults with self-reported ADHD who are fluent in Norwegian. The questionnaire’s brevity makes it a valuable resource in research and clinical settings. However, more studies are needed to test the instrument in a clinical sample.
School teachers' awareness of internet addiction in elementary school students: a regional survey in Japan
Authors: Kawabe Kentaro , Horiuchi Fumie , Hosokawa Rie , Nakachi Kiwamu , Soga Junya , Ueno Shu-ichi
Published: July 2023

In recent years, concerns about internet addiction (IA) among children have been increasing. This study focused on the awareness of IA in elementary school teachers. A web-based anonymous survey was conducted in November 2021. The participants completed an original questionnaire about their awareness of IA. The participants were divided into three groups based on their positions in the classroom: class teachers, support teachers, and administrative teachers. Out of 283 participants, over 70% had not approached students with IA and had little practical knowledge about the disorder. Support and administrative teachers had more opportunities to interact with students with IA than class teachers (p < 0.001 in both cases). Support teachers had more opportunities to ask their colleagues about IA than class teachers (p < 0.01); similarly, administrative teachers also had more opportunities to discuss IA with colleagues than class teachers (p = 0.04). Preventive interventions are recommended for people who communicate with children with IA. Students with IA might cause anxiety among teachers; therefore, preventive education strategies should be implemented with the cooperation of psychiatrists, psychologists, and public health nurses.
Psychiatrists’ attitudes towards functional neurological disorders: results from a national survey
Authors: Marotta Angela , Lasalvia Antonio , Fiorio Mirta , Zanalda Enrico , Di Sciascio Guido , Palumbo Claudia , Papola Davide , Barbui Corrado , Tinazzi Michele
Published: July 2023

Introduction

Functional neurological disorder (FND) presents motor, sensory, and cognitive symptoms characterized by clinical signs incongruent with known neurological disease. Together with other health professionals, like neurologists, psychiatrists can play an essential role in diagnosing and managing these disorders. Hence, understanding their opinion and clinical experience with FND is of utmost importance to catch potential educational needs and improve healthcare services for patients. This study aims at assessing the knowledge, opinion, and clinical approach of Italian psychiatrists to FND.

Methods

Members of the Italian Society of Psychiatry completed a 14-item web-based survey investigating their approach to FND. Results. Overall, 174 questionnaires were completed. Our main findings suggest that Italian psychiatrists have a psychogenetic conceptualization of FND. “Conversion disorders”, in fact, is the term most frequently used by Italian psychiatrists to refer to FND, thus implying a psychological etiology of these disorders. Congruently with this view, psychotherapy associated with pharmacological therapy is considered the most appropriate treatment by psychiatrists, while physiotherapy is an under-recognized treatment option for FND.

Discussion

The present study highlights that a psychogenetic view of FND dominates among Italian psychiatrists. This could be due to out-of-date knowledge about the pathophysiology of this group of disorders. Promoting education about novel approaches to FND would be of crucial importance to improving care for patients suffering from this condition.
Genomic epidemiology of dengue in Shantou, China, 2019
Authors: Tian Lina , Liang Chumin , Huang Xiaorong , Liu Zhe , Su Juan , Guo Chuan , Zhu Guanghu , Sun Jiufeng
Published: July 2023

Objectives

Dengue has been endemic in Southeast Asian countries for decades. There are few reports tracing the dynamics of dengue in real time. In this study, we generated hundreds of pathogen genomes to understand the genomic epidemiology of an outbreak in a hyper-endemic area of dengue.

Methods

We leveraged whole-genome short-read sequencing (PE150) to generate genomes of the dengue virus and investigated the genomic epidemiology of a dengue virus transmission in a mesoscale outbreak in Shantou, China, in 2019.

Results

The outbreak was sustained from July to December 2019. The total accumulated number of laboratory-confirmed cases was 944. No gender bias or fatalities were recorded. Cambodia and Singapore were the main sources of imported dengue cases (74.07%, n = 20). A total of 284 dengue virus strains were isolated, including 259 DENV-1, 24 DENV-2, and 1 DENV-3 isolates. We generated the entire genome of 252 DENV isolates (229 DENV-1, 22 DENV-2, and 1 DENV-3), which represented 26.7% of the total cases. Combined epidemiological and phylogenetic analyses indicated multiple independent introductions. The internal transmission evaluations and transmission network reconstruction supported the inference of phylodynamic analysis, with high Bayes factor support in BSSVS analysis. Two expansion founders and transmission chains were detected in CCH and LG of Shantou.

Conclusions

We observed the instant effects of genomic epidemiology in monitoring the dynamics of DENV and highlighted its prospects for real-time tracing of outbreaks of other novel agents in the future.
Self-reported medication adherence instruments and their applicability in low-middle income countries: a scoping review
Authors: Khoiry Qisty A. , Alfian Sofa D. , van Boven Job F. M. , Abdulah Rizky
Published: July 2023

Introduction

Medication non-adherence is an important public health issue, associated with poor clinical and economic outcomes. Globally, self-reported instruments are the most widely used method to assess medication adherence. However, the majority of these were developed in high-income countries (HICs) with a well-established health care system. Their applicability in low- and middle-income countries (LMICs) remains unclear. The objective of this study is to systematically review the applicability of content and use of self-reported adherence instruments in LMICs.

Method

A scoping review informed by a literature search in Pubmed, EBSCO, and Cochrane databases was conducted to identify studies assessing medication adherence using self-reported instruments for patients with five common chronic diseases [hypertension, diabetes, dyslipidemia, asthma, or Chronic Obstructive Pulmonary Disease (COPD)] in LMICs up to January 2022 with no constraints on publication year. Two reviewers performed the study selection process, data extraction and outcomes assessment independently. Outcomes focused on LMIC applicability of the self-reported adherence instruments assessed by (i) containing LMIC relevant adherence content; (ii) methodological quality and (iii) fees for use.

Findings

We identified 181 studies that used self-reported instruments for assessing medication adherence in LMICs. A total of 32 distinct types of self-reported instruments to assess medication adherence were identified. Of these, 14 self-reported instruments were developed in LMICs, while the remaining ones were adapted from self-reported instruments originally developed in HICs. All self-reported adherence instruments in studies included presented diverse potential challenges regarding their applicability in LMICs, included an underrepresentation of LMIC relevant non-adherence reasons, such as financial issues, use of traditional medicines, religious beliefs, lack of communication with healthcare provider, running out of medicine, and access to care. Almost half of included studies showed that the existing self-reported adherence instruments lack sufficient evidence regarding cross cultural validation and internal consistency. In 70% of the studies, fees applied for using the self-reported instruments in LMICs.

Conclusion

There seems insufficient emphasis on applicability and methodological rigor of self-reported medication adherence instruments used in LMICs. This presents an opportunity for developing a self-reported adherence instrument that is suitable to health systems and resources in LMICs.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD42022302215.
Epidemiology of herpes simplex virus type 1 in Canada: systematic review, meta-analyses, and meta-regressions
Authors: AlMukdad Sawsan , Harfouche Manale , Farooqui Uzma S. , Aldos Lana , Abu-Raddad Laith J.
Published: July 2023

Background

The objective of this study was to characterize herpes simplex virus type 1 (HSV-1) epidemiology in Canada.

Methods

HSV-1 publications as recent as December 6, 2021 were systematically reviewed, synthesized, and reported following PRISMA guidelines. Meta-analyses and meta-regressions were conducted.

Results

HSV-1 measures were extracted from 22 studies and included 32 overall seroprevalence measures (79 stratified), 2 overall proportions of HSV-1 detection in clinically diagnosed genital ulcer disease (2 stratified), and 8 overall proportions of HSV-1 detection in laboratory-confirmed genital herpes (27 stratified). Pooled mean seroprevalence was 19.1% [95% confidence interval (CI): 12.6–26.4%] among healthy children and 51.4% (95% CI: 47.3–55.5%) among healthy adults. Pooled mean seroprevalence among healthy general populations increased with age, with the lowest being 35.7% (95% CI: 29.1–42.6%) among individuals <20 years of age, and the highest being 70.0% (95% CI: 54.8–83.2) among individuals ≥40 years. Seroprevalence increased by 1.02-fold (95% CI: 1.01–1.04) per year. Pooled mean proportion of HSV-1 detection in genital ulcer disease was 30.8% (95% CI: 12.6–52.8%). Pooled mean proportion of HSV-1 detection in genital herpes was 37.4% (95% CI: 29.5–45.6%) and was highest in women and in young persons. Proportion of HSV-1 detection in genital herpes increased by 1.04-fold (95% CI: 1.00–1.08) per year.

Conclusions

HSV-1 epidemiology in Canada appears to be shifting toward less oral acquisition in childhood and more genital acquisition in adulthood, particularly among youth. Both HSV-1 seroprevalence and proportion of HSV-1 detection in genital herpes are increasing with time.
Knowledge and practice of infant exposure to sunlight among mothers in the rural villages of Mettu district, southwest Ethiopia
Authors: Tadesse Askalemariam , Yeshanew Solomon , Gedefa Abdi Geda
Published: July 2023

Background

Sunlight is essential for the synthesis of vitamin D and supports bone growth and strength. The awareness of mothers about the benefit of exposing their infants to sunlight, as well as the practice of doing so, is important to a child’s proper growth and development. The current study looked into mothers’ knowledge, practice, and factors related to infant sunlight exposure in rural villages in the Mettu district, southwest Ethiopia.

Methods

A community-based cross-sectional study design was utilized with randomly selected mothers of newborns from rural areas in the Mettu district. A structured questionnaire was used to collect information. Analysis of the data was performed using the SPSS version 21 software. Both descriptive and inferential analyses were carried out, and p < 0.05 was considered statistically significant.

Results

Among the 600 mothers who participated in the present study, 397 (66.2%) had good knowledge and 346 (57.7%) practiced proper exposure of their infants to sunlight. Out of the 482 mothers who practiced sunning, 382 (79.3%) did it daily, and 409 (84.8%) began sunning before 45 days of birth. However, 453 (94%) of the mothers used lubricants while sunbathing. Sociodemographic characteristics were found to have statistically significant associations with the level of knowledge and practice of mothers. Mothers with four to six children [AOR = 0.511, 95% CI: (0.352–0.741)] and those who got the information from health professionals [AOR = 3.285, 95% CI: (2.154, 5.011)] for the knowledge level, and mothers aged 36 years old and above [AOR = 0.801, 95% CI: (0.533, 0.161)], who were married during the data collection period [AOR = 0.370, 95% CI: (0.155, 0.884)], and employed by the government [AOR = 4.081, CI: (1.477, 11.280)] for the practice level were found to be significantly (p < 0.05) associated with the level of infant sunning.

Conclusion

Despite the fact that the majority of mothers in the study area had good knowledge and practice of infant sunlight exposure, there are clear signs that further work is needed to narrow the large gap seen in the present findings. Thus, district and zonal health offices, as well as stakeholders working with children, should start periodical maternity health education, and professional development training for health post workers.
Promise and unrealized potential: 10 years of the American Medical Association classifying obesity as a disease
Authors: Schumacher Leah M. , Ard Jamy , Sarwer David B.
Published: July 2023

Introduction

In June 2013, the American Medical Association (AMA), one of the most influential healthcare organizations in the United States, voted to recognize obesity as a disease. Many who supported this change believed that recognition by AMA of obesity as a disease would serve as a tipping point that would increase access to care, accelerate training and research on the prevention and treatment of obesity, and reduce weight stigma. On the 10-year anniversary of this vote, this perspective piece outlines key advances made, as well as unrealized potential, in improving the obesity public health landscape since the AMA’s classification of obesity as a disease.

Methods

We draw on the empirical literature, as well as our experiences as clinical psychologists, a physician, and public health researchers specializing in obesity, to provide an overview of major advances and continued challenges in improving access to obesity treatment, accelerating prevention and training, and reducing weight stigma. We also outline important next steps to advance these goals.

Results

While several notable advancements have occurred, significant work remains to create equitable access to evidence-based treatments, bring research and training on obesity on par with its prevalence, and reduce the pervasiveness and harm of weight stigma.

Conclusion

The past decade has witnessed some advances with respect to access to care and attention, yet there is unrealized potential that awaits attention. Truly conceptualizing and treating obesity as a chronic disease requires a major paradigm shift.
An intelligence coordination system toward creating the super-intelligent law firm
Authors: Kaomea Peter
Published: July 2023

A large law firm typically exhibits a collective intelligence comprised of hundreds or thousands of legal minds aimed at simultaneously engaging thousands of active matters across scores of industries and dozens of practice specialties with distinct doctrinal and procedural characteristics. The firm is challenged not only to achieve successful, cost-effective outcomes for its clients, but must also simultaneously, in competition with other firms and alternative service providers, attract and cultivate talent, develop and coordinate capabilities across multiple evolving areas of practice and continually improve a robust collective intelligence to gain a competitive edge. As various types of machine intelligences and tools are introduced, firms must also groom these into the collective. In this paper we explore a human-machine hybrid system for addressing this large scale, multi-dimensional, dynamic optimization challenge to coordinate a collective intelligence of humans and machines. Machine intelligence is needed to handle the computational complexity and it is complemented by human intelligence to help handle exceptions and novel situations. We believe this approach has potential for transforming the collective intelligence that is the large law firm.
Survey of electroencephalography usage and techniques for dogs
Authors: Luca Julia , McCarthy Samantha , Parmentier Thomas , Hazenfratz Michal , Linden Alex Zur , Gaitero Luis , James Fiona M. K.
Published: July 2023

Background

Canine epilepsy is a chronic common neurologic condition where seizures may be underreported. Electroencephalography (EEG) is the patient-side test providing an objective diagnostic criterion for seizures and epilepsy. Despite this, EEG is thought to be rarely used in veterinary neurology.

Objectives

This survey study aims to better understand the current canine EEG usage and techniques and barriers in veterinary neurology.

Methods

The online Qualtrics link was distributed via listserv to members of the American College of Veterinary Internal Medicine (ACVIM) Neurology Specialty and the European College of Veterinary Neurology (ECVN), reaching at least 517 veterinary neurology specialists and trainees worldwide.

Results

The survey received a 35% response rate, for a total of 180 participant responses. Fewer than 50% of veterinary neurologists are currently performing EEG and it is performed infrequently. The most common indication was to determine a discrete event diagnosis. Other reasons included monitoring treatment, determining brain death, identifying the type of seizure or epilepsy, localizing foci, sleep disorders, for research purposes, and post-op brain surgery monitorization. Most respondents interpreted their own EEGs. Clinical barriers to the performance of EEG in dogs were mainly equipment availability, insufficient cases, and financial costs to clients.

Conclusion

This survey provides an update on EEG usage and techniques for dogs, identifying commonalities of technique and areas for development as a potential basis for harmonization of canine EEG techniques. A validated and standardized canine EEG protocol is hoped to improve the diagnosis and treatment of canine epilepsy.
Immunomodulatory effects of canine mesenchymal stem cells in an experimental atopic dermatitis model
Authors: Kang Seok-Jin , Gu Na-Yeon , Byeon Jeong Su , Hyun Bang-Hun , Lee Jienny , Yang Dong-Kun
Published: July 2023

Mesenchymal stem cells (MSCs) have the potential to differentiate into multi-lineage cells, suggesting their future applicability in regenerative medicine and biotechnology. The immunomodulatory properties of MSCs make them a promising replacement therapy in various fields of animal research including in canine atopic dermatitis (AD), a skin disease with 10–15% prevalence. We investigated the immunomodulatory effects of MSCs in an experimental canine AD model induced by Dermatophagoides farinae extract ointment. Canine adipose tissue-derived MSCs (cAT-MSCs) were differentiated into mesodermal cell lineages at the third passage. Alterations in immunomodulatory factors in control, AD, and MSC-treated AD groups were evaluated using flow cytometric analysis, enzyme-linked immunosorbent assay, and quantitative reverse transcription PCR. In the MSC-treated AD group, the number of eosinophils decreased, and the number of regulatory T cells (Tregs) increased compared to those in the AD group. In addition, the immunoglobulin E (IgE) and prostaglandin E2 levels were reduced in the MSC-treated AD group compared to those in the AD group. Furthermore, the filaggrin, vascular endothelial growth factor, and interleukin-5 gene expression levels were relatively higher in the MSC-treated AD group than in the AD group, however, not significantly. cAT-MSCs exerted immunomodulatory effects in an AD canine model via a rebalancing of type-1 and -2 T helper cells that correlated with increased levels of Tregs, IgE, and various cytokines.
Comparison of Semi-Empirical Impedance Models for Locally-Reacting Acoustic Liners in a Wide Range of Sound Pressure Levels
Authors: Palchikovskiy Vadim , Kuznetsov Aleksandr , Khramtsov Igor , Kustov Oleg
Published: July 2023

A comparison is considered of the experimentally obtained impedance of locally reacting acoustic liner samples with the impedance calculated using semi-empirical Goodrich, Sobolev and Eversman models. The semi-empirical impedance models are outlined. In the experiment, the impedance is synchronously measured on a normal incidence impedance tube by the transfer function method and Dean’s method. A modification of the conventional normal incidence impedance tube is proposed to obtain these measurements. To automate the measurements, a program code is developed that controls sound generation and the recording of signals. The code includes an optimization procedure for selecting the voltage on an acoustic driver, providing the required sound pressure level on the face of the sample at different frequencies. The geometry of acoustic liner samples and specifics of synchronous impedance measurements by the aforementioned methods are considered. Experiments are performed at sound pressure levels from 100 to 150 dB in the frequency range of 500–3500 Hz. A comparative analysis of semi-empirical models with the experimental results at different sound pressure levels is carried out.
Improved Mass Flow Rate Regulation Methods Based on Variable Frequency Control: A Case Study of Oxidizer Agent Weighing for Solid Propellants
Authors: Lu Han , Wang Hongyu , Chen Xuhang , Bai Xinlin , Xu Zhigang , Wei Yaqiang , Fan Linlin
Published: July 2023

The feeding and weighing of oxidizer agents is the key process of solid rocket motor propellant preparation, and its accuracy directly affects the burning performance of solid rocket motors. At present, the existing multi-batch feeding methods have the problem of low accuracy and high time consumption of the oxidizer agent. In this paper, an improved mass flow rate regulation method based on variable frequency control is proposed to improve accuracy and reduce time consumption. The nonlinear variation process of the mass flow rate during the opening and closing process of the air-operated pinch valve is analyzed. The periodic opening and closing frequency of the air-operated pinch valve is introduced to establish the mathematical model of the mass flow rate and frequency, and then, the model parameters are obtained through the discrete element method. The plan of the method of variable frequency regulation and the frequency parameters were determined using the multi-objective optimization method. The experiments are carried out, and the results show that compared to the existing multi-batch feeding method, optimized with the improved mass flow rate regulation methods based on the variable frequency control method, improved the feeding and weighing accuracy by 0.37% and reduced time consumption by 25.6%.
Dynamic Modeling and Analysis of Spacecraft with Multiple Large Flexible Structures
Authors: Wei Jin , Liu Wei , Liu Jia , Yu Tao
Published: July 2023

An analytical dynamic model is presented for a spacecraft with multiple large flexible structures. Based on the partial differential equations (PDEs) of the motion of the solar panel and deployable arm, the governing equations of the main-body and deployable antenna and the boundary conditions at each end point are used to obtain the frequency and mode shapes of the system. Then, the ordinary differential equations (ODEs) of the system can be obtained from the orthogonality relations and mode shape. The influence of the deployable antenna on the frequencies and mode shapes of the spacecraft is investigated. The frequency veering and mode interchanged phenomenon are observed with the variation of the diameter of the deployable antenna. Using the ODEs, the dynamic responses of the spacecraft are calculated to study the influence of the control torque on the attitude and position of the antenna in the attitude maneuver.
Can the Orbital Debris Disease Be Cured Using Lasers?
Authors: Scharring Stefan , Kästel Jürgen
Published: July 2023

Ground-based high-power lasers are, in principle, able to de-orbit any kind of space debris object from the low Earth orbit (LEO) by remotely inducing laser-ablative momentum. However, the assessment of efficiency and operational safety depends on many factors, like atmospheric constraints or the risk of debris disintegration during irradiation. We analyze laser momentum for a great variety of target geometries and sizes and—for the first time in a large-scale simulation—include thermal constraints in the laser irradiation configuration. Using a coherently coupled 100 kJ laser system at 1030 nm wavelength and a 5 ns pulse duration in an optimized pointing elevation angle range, the pulse frequency should amount to less than 10 Hz to prevent fragment meltdown. For mechanically intact payloads or rocket bodies, repetition rates should be even lower. Small debris fragments sized between 10 and 40 cm can be de-orbited by employing around 100 to 400 station passes with head-on irradiation, while objects exceeding 2 m typically require far more than 1000 irradiations for de-orbit. Hence, laser-based debris removal cannot be considered a prime space sustainability measure to tackle the highest-risk large debris, yet it can provide the remediation of a multitude of small-sized debris using small networks of globally distributed laser sites.
Experimental Study on the Water Content Distribution of Profile Samples and the Improvement of Sampling Detection Methods
Authors: Tian Ye , Zhang Jiahang , Tang Junyue , Xu Wei , Zhang Weiwei , Tao Lijun , Jiang Shengyuan , Sun Yanbin
Published: July 2023

To provide reliable input information for the load design and extraction of lunar soil water ice samples, it is necessary to study the water content distribution and water migration of simulated lunar soil water ice samples. On this basis, the temperature field model and the hydrothermal coupling relationship are proposed. The temperature field model was constructed by combining energy conservation and Fourier’s heat transfer law. The coupling relationship was established, and the hydrothermal coupling model was obtained by testing the unfrozen water content using the nuclear magnetic resonance method. Finite element software was used to solve the model numerically, and the water migration rule of the soil water ice samples at different ambient temperatures were analyzed. Thin-wall drilling tests were carried out on the simulated lunar soil water ice samples to obtain water content data for different locations, and the simulation results were verified. Due to the migration effect of the cold end of the water, the closer we tested to the edge of the sample, the higher the water content was. The higher the ambient temperature was, the more pronounced the water migration phenomenon of the whole sample was. These research results provide a basis for sampling scheme design.
Optimization of the Fluted Force-Feed Seeder Meter with the Helical Roller Using the Discrete Element Method and Response Surface Analysis
Authors: Wang Jianxiao , Sun Wei , Simionescu Petru Aurelian , Ju Yuanjin
Published: July 2023

The seed metering process of a fluted force-feed seeder was simulated using the Discrete Element Method and its parameters optimized using the Box–Behnken Design of Experiments and the Response Surface Method. The rotational speed of the feed roller, the lead (helix) angle of the flutes, and the number of flutes were the independent variables, while the response value was the seeding uniformity index. Two regression models were investigated, and the following conclusions drawn. For the flute lead angle between 0 and 10 degrees, and the number of flutes between 10 and 14, it was found that the number of flutes and the lead angle influenced the seeding performance the most, with the order of importance being the (i) number of flutes, (ii) lead angle and (iii) roller speed. For the flute lead angle between 5 and 15 degrees, and the number of flutes between 12 and 16, it was found that the roller speed and the number of flutes influenced the seeding performance the most, with the order of importance being the (i) roller speed, (ii) number of flutes and (iii) flute lead angle. The two regression models were then minimized for the seeding uniformity index and the corresponding optima verified experimentally on a conveyor belt test stand fitted with an image recognition system.
Leaf Transcription Factor Family Analysis of Halophyte Glaux maritima under Salt Stress
Authors: Gu Rui , Wan Zhiqiang , Tang Fang , Shi Fengling , Yan Mengjiao
Published: July 2023

The reduction of crop yield caused by soil salinization has become a global problem. Halophytes improve saline alkali soil, and the halophyte transcription factors that regulate salt stress are crucial for improving salt tolerance. In this study, 1466 transcription factors were identified by transcriptome sequencing analysis of Glaux maritima leaves after salt stress (0, 600, and 800 mM/L NaCl). Their genes were distributed across 57 transcription factor families. KEGG and GO analyses showed significant enrichment in 14 pathways, with a total of 54 functions annotated. Gene expression analysis showed 820 differentially expressed genes distributed in 11 transcription factor families, including ERF, bHLH, WRKY, and NAC, and 8 expression modules. KEGG analysis revealed four genes with significant positive regulation: ABF2 (Unigene0078257) in the ABA signaling pathway, EIN3 (Unigene0000457 and Unigene0012139), and EIL1 (Unigene0042139) involved in ethylene signal transduction, and two with negative regulation, MYC1/2 (Unigene0009899 and Unigene0027167) in the main regulator of Jasmonic acid signal transduction. Protein–protein interaction networks suggested ABF2 and MYC1/2 as important transcription factors regulating G. maritima salt tolerance. Overall, the salt-tolerant transcription factors discovered in this study provide genetic resources for plant salt tolerance inheritance, and lay a theoretical foundation for the study of the salt-tolerant molecular mechanism of the halophyte Glaux maritima.
Cotton Gin Stand Machine-Vision Inspection and Removal System for Plastic Contamination: Auto-Calibration Design
Authors: Pelletier Mathew G. , Wanjura John D. , Holt Greg A. , Kothari Neha
Published: July 2023

Plastic contamination in marketable cotton bales, predominantly from plastic wraps used in John Deere round module harvesters, poses a significant challenge to the U.S. cotton industry. Despite rigorous manual efforts, the intrusion of plastic into the cotton gin’s processing system persists. We have developed a machine-vision detection and removal system aimed at mitigating this problem. This system employs inexpensive color cameras to detect plastic on the gin-stand feeder apron and subsequently removes it, reducing contamination. This system, built around low-cost ARM computers running Linux, comprises 30–50 machines and requires considerable effort to calibrate and tune. Moreover, its operation represents a technological challenge to typical cotton gin workers. This research presents a solution to this calibration operational hurdle by introducing an auto-calibration algorithm that has potential to simplify the system into a plug-and-play device. The auto-calibration system is designed to dynamically track the cotton color and utilizes frequency statistics to avoid plastic images that could compromise the system’s performance if incorporated into the auto-calibration process. We detail the design of the auto-calibration algorithm, which is expected to significantly reduce the setup overhead and facilitate the system’s continuous use. This innovation minimizes the need for skilled personnel and, therefore, is expected to expedite the system’s adoption across the cotton ginning industry.
Sexual Reproduction Is Not Responsible for Caragana Shrub Encroachment in Grasslands
Authors: Xie Lina , Li Yuchen , Lin Mingyan , Guo Hongyu , Wang Yue , Wang Lihong , Ma Chengcang
Published: July 2023

Shrubs tended to increase their abundance as climatic aridity and grazing intensity increased in the Inner Mongolian grassland. Increasing shrub abundance was believed to be due to enhanced reproduction. However, the effects of climatic aridity and grazing on the sexual reproduction of shrubs in grassland remain largely unclear. In this study, we conducted field experiments with Caragana microphylla to examine the variation of sexual reproduction aspects (seed production, seed vigor, and sapling establishment) along a climatic aridity gradient (subhumid, semiarid, arid, and dry arid zones) and a grazing intensity gradient (fenced, mildly grazed, and severely grazed). We then quantified the population growth rate based on seed production and sapling establishment rates. Our objective was to evaluate whether sexual reproduction is the main mechanism for Caragana encroachment into grasslands. We found that climatic aridity decreased seed quantity and seed vigor but increased the sapling establishment rate of Caragana shrubs. Under ungrazed conditions, climatic aridity did not affect population growth rates, while under grazing conditions, increased aridity stresses reduced population growth rates. Grazing reduced seed production, sapling establishment, and population growth rates. Climatic aridity enhanced the negative effects of grazing on sexual reproduction, while grazing intensified the negative effects of aridity on the population growth of Caragana shrubs. In conclusion, climatic aridity, grazing, and their combined effects had negative effects on the sexual reproduction of Caragana shrubs. Therefore, sexual reproduction could not fully explain the increased abundance of shrubs with increasing aridity and grazing. Clonal reproduction might be of considerable importance for understanding the mechanism of shrub encroachment in grasslands.
Effect of Environmental, Soil and Management Factors on Weed Flora of Field Pea in South-East Hungary
Authors: Kovács Endre Béla , Dorner Zita , Csík Dávid , Zalai Mihály
Published: July 2023

Pea is a widely cultivated leguminous plant which also contributes to soil enrichment through nitrogen fixation and benefits crop rotations. However, large weed populations are a challenge for pea production, requiring effective management strategies. It is essential to highlight the influence of soil parameters, factors affecting the environment, and management practices on weed populations to develop effective weed control and maximize pea yield and ease of harvesting. In our study, a total of 31 pea fields were surveyed prior to harvest to determine the coverage of each weed species, with the aim of identifying the typical weeds in the study area. In addition, environmental, soil, and management factors were recorded for each field. Based on our hypotheses, these factors influence the weed composition, and these effects can be described by the dominance of weed species. In our study, summer annuals and geophytic perennials were common, with Echinochloa crus-galli and Convolvulus arvensis being most dominant. The analysis revealed that the year of data record, soil type, and farming system most significantly influenced weed composition. Weed species were observed to have varying responses to soil texture, salt concentration, and phosphorus content. The survey period, geographical factors, farming system, and tillage practices also played a role in determining weed flora. The findings suggest strong correlations between soil parameters and weed composition, highlighting the importance of soil management in weed control. The year of data collection had the greatest influence on weed infestation. Soil-related variables, such as soil type, also played a significant role. Farming systems had a smaller effect on weed composition. Comparing our results with previous country level weed surveys in Hungary, our results identified some unique characteristics in the weed flora of South-East Hungary.
Analysis and Prediction of the Impact of Socio-Economic and Meteorological Factors on Rapeseed Yield Based on Machine Learning
Authors: Liang Jiaping , Li Hang , Li Na , Yang Qiliang , Li Linchao
Published: July 2023

Rapeseed is one of China’s major oil crops, and accurate yield forecasting is crucial to the growth of the rapeseed industry and the country’s food security. In this study, the data on natural and socio-economic factors from 2001 to 2020 and the yield of rapeseed in China were used as the data basis. The Pearson correlation coefficient was used to analyze the relationship between the influencing factors and the yield of rapeseed, and the prediction effect of four machine learning models (linear regression (LR), decision tree (DTR), random forest (RF), and support vector machine (SVM)) on the yield of rapeseed was compared in China’s main rapeseed-producing area. The results demonstrate that the yield of rapeseed in China showed an increasing trend, but fluctuated greatly. Rural electricity consumption, gross agricultural production, the net amount of agricultural fertilizer application, effective irrigation area, total power of agricultural machinery, and consumption of agricultural plastic film had a positive effect on the increase in rapeseed yield. However, due to the impact of climate change and disasters, the yield of rapeseed has had significant fluctuations. A Pearson correlation analysis showed that socio-economic factors (rural electricity consumption, gross agricultural production, effective irrigation area, total power of agricultural machinery, consumption of agricultural plastic film, etc.) played a dominant role in rapeseed yield changes. The RF model had a good prediction effect on rapeseed yield, and natural factors and socio-economic factors had different effects on spring rapeseed and winter rapeseed. Winter rapeseed yield was mainly affected by socio-economic factors, accounting for as high as 89% of the importance. Among them, the sown area of rapeseed and the effective irrigation area had the greatest impact. The effects of natural factors and socio-economic factors on spring rapeseed yield were similar, accounting for 47% and 53%, respectively, and the mean annual precipitation, sunshine duration, and sown area of rapeseed were the most influential variables.
An Overview of Fruit Allergens: Structural, Functional, Phylogenetical, and Clinical Aspects
Authors: Barre Annick , Benoist Hervé , Rougé Pierre
Published: July 2023

Most of the allergenic proteins from fruits identified so far belong to different families of pathogenesis-related (PR) proteins. These PR proteins have been classified in different families of structurally and functionally unrelated proteins, but the majority of all fruit allergens belong to three groups, in particular PR-5 thaumatin-like proteins (TLP), PR-10 Bet v 1-like proteins, and PR-14 non-specific lipid transfer proteins (nsTLP). Some allergenic proteins from fruits can also be found among PR-protein families of PR-2 β1,3-glucanase proteins, PR-3 chitinases I, II, IV–VII, and PR-8 chitinases III. In addition, other important fruit allergens occur in protein families unrelated to the PR-protein families, such as the profilins and the newly emerging group of gibberellin-regulated proteins (GBRP). Finally, proteins that belong to seed storage proteins from higher plants, including 2S albumins, 7S globulins (vicilin), and 11S globulins (legumin), must be retained as possible potential fruit allergens resulting from the unintended consumption of the seeds. Here, we present an overview of the structural organization, functional properties, and phylogenetical relationships among these different groups of fruit allergens, supporting the occurrence of cross-reactivity and cross-allergenicity often described between fruit allergens, and the corresponding allergens from vegetables and pollens.