bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of 4-DOF Voice Coil Motor with Function of Reducing Laser Geometrical Fluctuations
Authors: Liu Chien-Sheng , Wu Yu-Cheng , Lan Yu-Jie
Published: December 2021

In order to improve the quality of the laser and shorten the optical path of the fast steering mirror (FSM) laser compensation system, this paper proposes a four-degrees-of-freedom (4-DOF) voice coil motor (VCM) with the function of reducing laser geometrical fluctuations. The feature of this paper is the combination of a DC brushed spindle motor and the proposed 4-DOF VCM. A diffuser is installed on the shaft of the DC brushed motor for suppressing the laser speckle. The proposed 4-DOF VCM is combined with a laboratory-designed mirror set, controlling the laser direction to compensate for laser fluctuations. The proposed actuator was designed and verified by using the commercial CAD software SolidWorks and finite element analysis (FEA) software ANSYS. A mathematical model was built to simulate the dynamic response of the proposed 4-DOF VCM in MATLAB Simulink.
Influence of Hydraulics on Electric Drive Operational Characteristics in Pump-Controlled Actuators
Authors: Zakharov Viacheslav , Minav Tatiana
Published: December 2021

Transitioning from a valve- to pump-controlled system has been observed in working hydraulics to reduce energy consumption and accelerate the response to decarbonization requirements in Non-road Mobile Machinery (NRMM). The utilization of an electric motor as a prime mover significantly enhances the chances of completing this task. While the concept of combining hydraulics with an electric motor is not new, the functionality of electro-hydraulics can be improved due to multidisciplinary domains, the impact of the electric drive and its control is usually underestimated. Thus, this study aims to evaluate the influence of hydraulics on electric drive operational characteristics in pump-controlled actuators. It utilizes an electro-hydraulic model and simulation study under various conditions, a stability analysis, and a Pulse Width Modulation (PWM) of the frequency converter (FC) evaluation on a selected variable speed pump-controlled actuator to demonstrate the influence of hydraulics on an electric drive. Experimental validation of the model demonstrated an acceptable accuracy of 5%. Moreover, the stability analysis indicated a rise time of about 0.051 s, an overshoot of 0.53%, a transient process time of 0.13 s, and a steady-state value difference of 0.16%; all of which guarantees stable operation of the electric drive with a hydraulic load. In addition, an optimal PWM of an FC frequency of 5 kHz was selected to guarantee accurate speed control with minimum overshoot.
Sensitivity-Based Non-Linear Model Predictive Control for Aircraft Descent Operations Subject to Time Constraints
Authors: Dalmau Ramon , Prats Xavier , Baxley Brian
Published: December 2021

The ability to meet a controlled time of arrival while also flying a continuous descent operation will enable environmentally friendly and fuel efficient descent operations while simultaneously maintaining airport throughput. Previous work showed that model predictive control, a guidance strategy based on a reiterated update of the optimal trajectory during the descent, provides excellent environmental impact mitigation figures while meeting operational constraints in the presence of modeling errors. Despite that, the computational delay associated with the solution of the trajectory optimization problem could lead to performance degradation and stability issues. This paper proposes two guidance strategies based on the theory of neighboring extremals that alleviate this problem. Parametric sensitivities are obtained by linearization of the necessary conditions of optimality along the active optimal trajectory plan to rapidly update it for small perturbations, effectively converting the complex and time consuming non-linear programming problem into a manageable quadratic programming problem. Promising results, derived from more than 4000 simulations, show that the performance of this method is comparable to that of instantaneously recalculating the optimal trajectory at each time sample.
Expression Analysis of DgD14, DgBRC1 and DgLsL in the Process of Chrysanthemum Lateral Bud Formation
Authors: Luo Cheng , Wang Xin-Jie , Ran Ai-Ning , Song Jing-Jing , Li Xin , Ma Qi-Qi , Pan Yuan-Zhi , Liu Qing-Lin , Jiang Bei-Bei
Published: December 2021

The growth of lateral bud can greatly affect the development of apical bud and reduce the quality of single-flower cut chrysanthemum. However, the wide use of artificial bud removal in production leads to the increase on production cost. Therefore, it is important to study the lateral bud development mechanism in chrysanthemum for plant type regulation and genetic improvement. Auxin (IAA), cytokinins (CKs) and strigolactones (SLs) have direct or indirect effects on the formation of lateral buds. D14, BRC1 and LsL are key factors regulating the signal pathways of hormones, but their regulation mechanisms on the development of lateral buds in chrysanthemum are still unclear. In this study, single-flower cut chrysanthemum ‘Jinba’ and spray cut chrysanthemum ‘Fenyan’ were used as experimental materials. Quantitative real-time PCR was used to observe the effects of apical bud removal and exogenous hormones on the growth of lateral buds and the expression levels of DgD14, DgBRC1 and DgLsL, so as to clarify the expression characteristics of three genes in the process of lateral bud formation. The results showed that GA was effective in promoting the growth of lateral buds, whereas IAA and ABA had little effects on lateral bud growth or even inhibited. Removing apical dominance can significantly affect the expression levels of three genes, which regulated the formation and elongation of lateral buds. Additionally, the three genes showed different responses to different hormone treatments. DgD14 had a significant response to GA, but a gentle response to ABA. The expression levels of DgBRC1 varied in different trends, and it responded to IAA in a more dramatic way. The levels of DgLsL reached the peaks quickly before decreased in most experimental groups, and its response to GA was extraordinary severe.
Factors Influencing the Adoption of Climate-Smart Irrigation Technologies for Sustainable Crop Productivity by Smallholder Farmers in Arid Areas of South Africa
Authors: Serote Batizi , Mokgehle Salmina , Du Plooy Christian , Mpandeli Sylvester , Nhamo Luxon , Senyolo Grany
Published: December 2021

The adoption of climate-smart irrigation technologies amongst smallholder farmers generally remains low beside their role in combating food and nutrition security in a society and in climate change adaptation strategies. This study identified the factors influencing smallholder farmers’ decision to adopt Climate Smart Irrigation Technologies (CSIT) in the Limpopo Province of South Africa. Data were collected through the completion of a face-to-face structured questionnaire by 100 smallholder farmers selected through convenience and purposive sampling. A probit regression and OLS model were used to identify factors that influence the smallholder farmer’s decision to adopt CSIT and the level of adoption. The results indicated that only 46% of the smallholder farmers adopted climate-smart irrigation technologies and suggested that adoption is influenced positively by factors such as gender, age, district, farm size, staple food production and knowledge on CSIT. There is an urgent need for related stakeholders to transform the smallholder farmer subsector through improved extension services, training, adopting resilient crop varieties, promoting underutilized and nutrient-dense crops adapted to harsh local conditions, and other interventions. This should be done by promoting awareness to smallholder farmers regarding these interventions and new technologies that have the potential to improve rural livelihoods and enhance resilience and adaptation.
Continuous Cropping Changes the Composition and Diversity of Bacterial Communities: A Meta-Analysis in Nine Different Fields with Different Plant Cultivation
Authors: Alami Mohammad Murtaza , Pang Qiuling , Gong Zedan , Yang Tewu , Tu Daiqun , Zhen Ouyang , Yu Weilong , Alami Mohammad Jawad , Wang Xuekui
Published: December 2021

Chinese goldthread (Coptis chinensis Franch.) represents one of the most important medicinal plants with diverse medicinal applications, but it easily suffers from continuous cropping obstacles in the plantation. In this study, we have selected eight different continuously cropped fields with C. chinensis and fallow field, providing detailed information regarding the diversity and composition of the rhizospheric bacterial communities. We have found a significant difference between fallow field (LH) and other continuously cropped fields in soil pH; the total content of nitrogen, phosphorus, and potassium; and soil enzyme activities. The results indicate that continuous cropping had a significant effect on soil physicochemical properties and enzyme activities under different plant cultivations. The relative abundance of bacterial phyla was significantly altered among the fields; for example, proteobacteria and Actinobacteria were observed to be higher in continuous cropping of maize (HY6) and lower in sweet potato continuous cropping (HH). Alpha diversity analysis showed that different plants with different years of continuous cropping could change the diversity of bacterial communities, among which the effect of maize and Polygonum multiflorum continuous cropping were most significant. Principle coordinate analysis (PCoA) showed that continuously cropped C. chinensis (LZ) and cabbage continuously cropped for 2 years (HS) were slightly clustered together and separated from LH and others. The results showed that the similarity of the bacterial community in the same crop rotation was higher, which further indicated that the bacterial community structure was significantly altered by the continuous cropping system and plant species. Our study provides a foundation for future agricultural research to improve microbial activity and increase crops/cash-crops productivity under a continuous cropping system and mitigate continuous cropping obstacles.
A Quadratic Regression Model to Quantify Plantation Soil Factors That Affect Tea Quality
Authors: Wen Bo , Li Ruiyang , Zhao Xue , Ren Shuang , Chang Yali , Zhang Kexin , Wang Shan , Guo Guiyi , Zhu Xujun
Published: December 2021

Tea components (tea polyphenols, catechins, free amino acids, and caffeine) are the key factors affecting the quality of green tea. This study aimed to relate key biochemical substances in tea to soil nutrient composition and the effectiveness of fertilization. Seventy tea samples and their corresponding plantation soil were randomly collected from Xinyang City, China. The catechins, free amino acids, and caffeine in tea were examined, as well as the soil pH, nitrate (NO3--N), ammonium (NH4+-N), available phosphorus (AP), available potassium (AK), and soil organic matter (SOM). The ordinary kriging was employed to visualize the spatial variation characteristic by ArcGIS. A quadratic regression model was used to analyze the effects of the soil environment on the tea. The results showed that the soil pH of the study area was suitable for cultivating tea plants. The relationship between soil pH and tea polyphenols and catechins presented the U-shape curve, whereas the soil pH and NH4+-N and the free amino acids, the soil pH, and caffeine presented the inverted U-shape curve. Soil management measures could be implemented to control the soil environment for improving the tea quality. The combination of the macro metrological model with individual experimentation could help to analyze the detailed influence mechanisms of environmental factors on plant physiological processes.
Quality of Chopped Maize Can Be Improved by Processing
Authors: Jančík Filip , Kubelková Petra , Kumprechtová Dana , Loučka Radko , Homolka Petr , Koukolová Veronika , Tyrolová Yvona , Výborná Alena
Published: December 2021

The aim of this study was to assess the effect of different maize processing technologies, comparing treatments with conventional rollers (control), MCC MAX rollers and a Shredlage crop processor on the quality of chopped maize. All the three types of chopped maize were harvested on the same day from the same field where the same maize hybrid was grown. The chemical composition of chopped maize, degree of grain processing and particle size fractions, and the effect of the treatments on rumen disappearance of dry matter, organic matter, NDF and starch were assessed. The highest degree of grain processing was achieved with the Shredlage processor (79.2%), and at the same time this processing method had a tendency to produce the highest proportion of physically effective fibre (37.2%). Compared to the conventional rollers (control), the chopped maize produced using the Shredlage processor had higher disappearance of dry matter, organic matter and NDF after 48 h of rumen incubation and of starch after 24 h of incubation. The MCC MAX rollers provided higher disappearance of all nutrients compared to the control treatment after both 24 and 48 h of incubation.
Assessment of Spatiotemporal Variations in the Water Quality of the Han River Basin, South Korea, Using Multivariate Statistical and APCS-MLR Modeling Techniques
Authors: Cho Yong-Chul , Choi Hyeonmi , Yu Soon-Ju , Kim Sang-Hun , Im Jong-Kwon
Published: December 2021

This study evaluated the spatiotemporal variability of water quality in the Han River Basin (HRB) as well as the contributions of potential pollution sources using multivariate statistical and absolute principal component score-multiple linear regression (APCS-MLR) modeling techniques. From 2011 to 2020, data on water quality parameters were collected from 14 sites in the Ministry of Environment’s water quality monitoring network. Using spatiotemporal cluster analysis, these sites were classified into two periods over the year (dry and wet seasons) and into three regions: low pollution region (LPR), moderate pollution region (MPR), and high pollution region (HPR). Through principal component analysis, we identified four potential factors accounting for 80.1% and 74.1% of the total variance in the LPR and MPR, respectively, and three that accounted for 72.7% of the total variance in the HPR. APCS-MLR results indicated domestic sewage and phytoplankton growth (25%), domestic sewage and seasonal influence (29%), and point pollution sources caused by domestic sewage and industrial wastewater discharge (31%) as potential factors for the LPR, MPR, and HPR. These results demonstrate that the multivariate statistical techniques and the APCS-MLR model can be effectively used to monitor network design, quantitatively evaluate potential pollution sources, and establish efficient water quality management policies.
Peer Irrigators and the Choice of Field Management and Water Control Practices for Irrigation in Arkansas
Authors: Bailey Victoria , Kovacs Kent , Henry Christopher , Huang Qiuqiong , Krutz Larry J.
Published: December 2021

We examined how irrigation techniques in use by family and friends influence the use and share of land utilizing different irrigation techniques by Arkansas producers. A bivariate sample selection model simultaneously estimated how farm characteristics determine the use and explain the share of a farm that utilizes an irrigation technique. We found that the irrigation techniques in use by family and friends do affect the irrigation techniques a producer uses and the share of acres utilizing different irrigation techniques. A producer with a family or friend that uses end-blocking irrigation is 41% more likely to use end-blocking themselves. Having a family or friend who uses pivot irrigation technology tends to decrease the share of irrigated acres that utilizes end block irrigation by 0.211. We also found that when the irrigation techniques in use by family and friends interact with variables such as location and participation in a regional conservation partnership program, the effects on the producer’s decision vary. The share of irrigated acres that use cutback irrigation decreases by 0.21 for a producer who has a peer that uses irrigation scheduling. However, if the producer lives along Crowley’s Ridge and has a peer that uses irrigation scheduling, the share of irrigated acres that use cutback irrigation decreases by an additional 0.54.
Soil Organic Carbon Sequestration after Biochar Application: A Global Meta-Analysis
Authors: Gross Arthur , Bromm Tobias , Glaser Bruno
Published: December 2021

Biochar application to soil has the potential to sequester carbon in the long term because of its high stability and large-scale production potential. However, biochar technologies are still relatively new, and the global factors affecting the long-term fate of biochar in the environment are still poorly understood. To fill this important research gap, a global meta-analysis was conducted including 64 studies with 736 individual treatments. Field experiments covered experimental durations between 1 and 10 years with biochar application amounts between 1 and 100 Mg ha−1. They showed a mean increase in soil organic carbon (SOC) stocks by 13.0 Mg ha−1 on average, corresponding to 29%. Pot and incubation experiments ranged between 1 and 1278 days and biochar amounts between 5 g kg−1 and 200 g kg−1. They raised SOC by 6.3 g kg−1 on average, corresponding to 75%. More SOC was accumulated in long experimental durations of >500 days in pot and incubation experiments and 6–10 years in field experiments than in shorter experimental durations. Organic fertilizer co-applications significantly further increased SOC. Biochar from plant material showed higher C sequestration potential than biochar from fecal matter, due to higher C/N ratio. SOC increases after biochar application were higher in medium to fine grain textured soils than in soils with coarse grain sizes. Our study clearly demonstrated the high C sequestration potential of biochar application to agricultural soils of varying site and soil characteristics.
Correlation between Metabolic Rate and Salinity Tolerance and Metabolic Response to Salinity in Grass Carp (Ctenopharyngodon idella)
Authors: Djiba Pathe Karim , Zhang Jianghui , Xu Yuan , Zhang Pan , Zhou Jing , Zhang Yan , Luo Yiping
Published: December 2021

The metabolic rate could be one of the factors affecting the salinity tolerance capacity of fish. Experiment I tested whether metabolic rates correlate with the upper salinity tolerance limit among individual grass carp by daily increasing salinity (1 g kg−1 day−1). The feeding dropped sharply as the salinity reached 10 g kg−1 and ceased when salinities exceeded 11 g kg−1. The ventilation frequency decreased weakly as salinity increased from 0 to 12 g kg−1 and then increased rapidly as salinity reached 14 g kg−1. The fish survived at salinities lower than 14 g kg−1, and all fish died when salinity reached 17 g kg−1. The upper salinity tolerance limit was not correlated with metabolic rates. Therefore, a lower metabolic rate may not necessarily allow for better salinity tolerance capacity. Experiment II tested how different salinities (0, 0.375, 0.75, 1.5, 3, and 6 g kg−1 for 2 weeks) affect the metabolic parameters of grass carp. The changes in the resting metabolic rate with increasing salinity could be explained by the relative changes in interlamellar cell mass and protruding lamellae. The maximum metabolic rate remained constant, suggesting that the salinity-induced changes in the gill surface had a minor effect on oxygen uptake capacity.
Characterization of Oviduct Lining, with Emphasis on the Sperm Storage Tubule Region (Uterovaginal Junction), Correlated with Fertility in Mature and Old Thai Native Hens
Authors: Kheawkanha Theerapat , Boonkum Wuttigrai , Vongpralub Thevin , Chankitisakul Vibuntita
Published: December 2021

The effect of age on fertility was investigated in Thai native chickens. The objective of this study was to determine the effects of age (mature and old) on the morphological characteristics of the reproductive organs and the histological characteristics of the uterovaginal junction (UVJ) tissues, resident sperm in the UVJ, and fertility duration in Thai native hens. We found no differences in the morphological characteristics of the reproductive organs, except for the number of follicles and the sizes of the fifth large yellow follicle in mature hens, which were greater than those in old hens (p < 0.05). The diameter of the sperm storage tubules (SSTs) epithelium was larger in old hens than in mature hens (p < 0.05), whereas the epithelium height was lower in old hens (p < 0.05). The number of sperm in the SSTs was greater in mature hens compared with old hens (p < 0.05). Mature hens showed a higher fertility rate than old hens. Our results suggest that, in old hens, the function of the SSTs is impaired, and sperm cannot be retained. Such a deterioration of the SSTs may be one of the factors involved in the decline in fertility.
Anisakis pegreffii Larvae in Sphyraena viridensis and Description of Granulomatous Lesions
Authors: De Benedetto Giovanni , Giannetto Alessia , Riolo Kristian , Iaria Carmelo , Brianti Emanuele , Gaglio Gabriella
Published: December 2021

The aim of the present study was to describe gastric granuloma caused by Anisakis pegreffii in Sphyraena viridensis caught in the central Mediterranean Sea. Sixty-eight S. viridensis specimens were collected from different fish markets on the east coast of Sicily. Coelomic organs were observed both macroscopically and with the aid of stereomicroscope. Parasite specimens and lesioned tissues were collected for identification, histological and molecular analyses. Twelve specimens (p = 17.6%) were positive for the presence of nematode larvae, morphologically identified as larvae of Anisakis sp., with values of mean abundance and mean intensity of 0.9 and 4.8, respectively. One large female specimen showed massive parasite infection associated with nodular lesions of the gastric wall. By histology, several nematode larvae encysted through the gastric wall were found. The parasite bodies were surrounded by a granulomatous reaction made up of macrophages, epithelioid cells, some lymphocytes and an external connective sheet. Molecular analysis of 18S rRNA and cox2 genes from Anisakis sp. collected larvae, identified them as A. pegreffii. The lesions here described, though macroscopically superimposable on human eosinophilic granuloma, microscopically showed significant differences in the inflammatory cells involved and in the type of immune reaction.
Welfare of Farmed Crocodilians: Identification of Potential Animal-Based Measures Using Elicitation of Expert Opinion
Authors: Hewitt Leisha , Small Alison
Published: December 2021

Animal-based measures are the measure of choice in animal welfare assessment protocols as they can often be applied completely independently to the housing or production system employed. Although there has been a small body of work on potential animal-based measures for farmed crocodilians, they have not been studied in the context of an animal welfare assessment protocol. Potential animal-based measures that could be used to reflect the welfare state of farmed crocodilians were identified and aligned with the Welfare Quality® principles of good housing, good health, good feeding and appropriate behaviour. A consultation process with a panel of experts was used to evaluate and score the potential measures in terms of validity and feasibility. This resulted in a toolbox of measures being identified for further development and integration into animal welfare assessment on the farm. Animal-based measures related to ‘good feeding’ and ‘good health’ received the highest scores for validity and feasibility by the experts. There was less agreement on the animal-based measures that could be used to reflect ‘appropriate behaviour’. Where no animal-based measures were deemed to reliably reflect a welfare criterion nor be useful as a measure on the farm, additional measures of resources or management were suggested as alternatives. Future work in this area should focus on the reliability of the proposed measures and involve further evaluation of their validity and feasibility as they relate to different species of crocodilian and farming system.
Dietary Probiotic Pediococcus acidilactici MA18/5M Improves the Growth, Feed Performance and Antioxidant Status of Penaeid Shrimp Litopenaeus stylirostris: A Growth-Ration-Size Approach
Authors: Castex Mathieu , Leclercq Eric , Lemaire Pierrette , Chim Liêt
Published: December 2021

Probiotics are increasingly documented to confer health and performance benefits across farmed animals. The aim of this study was to assess the effect of a constant daily intake of the single-strain probiotic Pedicococcus acidilactici MA18/5M (4 × 108 CFU.day−1.kg−1 shrimp) fed over fixed, restricted ration sizes (1% to 6% biomass.day−1) on the nutritional performance and metabolism of adult penaeid shrimp Litopenaeus stylirostris (initial body-weight, BWi = 10.9 ± 1.8 g). The probiotic significantly increased the relative daily growth rate (RGR) across all ration size s tested (Mean-RGR of 0.45 ± 0.08 and 0.61 ± 0.07% BWi.day−1 for the control and probiotic groups, respectively) and decreased the maintenance ration (Rm) and the optimal ration (Ropt) by 18.6% and 11.3%, respectively. Accordingly, the probiotic group exhibited a significantly higher gross (K1) and net (K2) feed conversion efficiency with average improvement of 35% and 30%, respectively. Enhanced nutritional performances in shrimps that were fed the probiotic P. acidilactici was associated with, in particular, significantly higher α-amylase specific activity (+24.8 ± 5.5% across ration sizes) and a concentration of free-glucose and glycogen in the digestive gland at fixed ration sizes of 3% and below. This suggests that the probiotic effect might reside in a better use of dietary carbohydrates. Interestingly, P. acidilactici intake was also associated with a statistically enhanced total antioxidant status of the digestive gland and haemolymph (+24.4 ± 7.8% and +21.9 ± 8.5%, respectively; p < 0.05). As supported by knowledge in other species, enhanced carbohydrate utilization as a result of P. acidilactici intake may fuel the pentose-phosphate pathway, generating NADPH or directly enhancing OH-radicals scavenging by free glucose, in turn resulting in a decreased level of cellular oxidative stress. In conclusion, the growth-ration method documented a clear contribution of P. acidilactici MA18/5M on growth and feed efficiency of on-growing L. stylirostris that were fed fixed restricted rations under ideal laboratory conditions. The effect of the probiotic on α-amylase activity and carbohydrate metabolism and its link to the shrimp’s antioxidant status raises interesting prospects to optimize dietary formulations and helping to sustain the biological and economic efficiency of the penaeid shrimp-farming industry.
Combining Analytical Approaches and Multiple Sources of Information to Improve Interpretation of Diagnostic Test Results for Tuberculosis in Wild Meerkats
Authors: Patterson Stuart J. , Clarke Charlene , Clutton-Brock Tim H. , Miller Michele A. , Parsons Sven D. C. , Pfeiffer Dirk U. , Vergne Timothée , Drewe Julian A.
Published: December 2021

Diagnostic tests are used to classify individual animals’ infection statuses. However, validating test performance in wild animals without gold standard tests is extremely challenging, and the issue is further complicated in chronic conditions where measured immune parameters vary over time. Here, we demonstrate the value of combining evidence from different diagnostic approaches to aid interpretation in the absence of gold standards, large sample sizes, and controlled environments. Over a two-year period, we sampled 268 free-living meerkats (Suricata suricatta) longitudinally for Mycobacterium suricattae (a causative agent of tuberculosis), using three ante-mortem diagnostic tests based on mycobacterial culture, and antigen-specific humoral and cell-mediated immune responses, interpreting results both independently and in combination. Post-mortem cultures confirmed M. suricattae infection in 22 animals, which had prior ante-mortem information, 59% (13/22) of which were test-positive on a parallel test interpretation (PTI) of the three ante-mortem diagnostic assays (95% confidence interval: 37–79%). A similar ability to detect infection, 65.7% (95% credible interval: 42.7–84.7%), was estimated using a Bayesian approach to examine PTI. Strong evidence was found for a near doubling of the hazard of death (Hazard Ratio 1.75, CI: 1.14–2.67, p = 0.01), associated with a positive PTI result, thus demonstrating that these test results are related to disease outcomes. For individual tests, small sample sizes led to wide confidence intervals, but replication of conclusions, using different methods, increased our confidence in these results. This study demonstrates that combining multiple methodologies to evaluate diagnostic tests in free-ranging wildlife populations can be a useful approach for exploiting such valuable datasets.
The Source Matters–Effects of High Phosphate Intake from Eight Different Sources in Dogs
Authors: Dobenecker Britta , Kienzle Ellen , Siedler Stephanie
Published: December 2021

Elevated serum phosphate concentrations are an established risk factor for cardiovascular disease and mortality in chronic kidney disease in various species. Independent associations of other parameters of phosphorus metabolism, such as phosphorus intake from different sources and serum concentrations of phosphorus, as well as parameters involved in the regulation, such as parathyroid hormone (PTH) or markers of bone turnover, have been studied in less detail. Therefore, the serum kinetics of phosphate, PTH, and the bone resorption marker bone-specific alkaline phosphatase (BAP) were investigated after 18 days of feeding a control diet and diets supplemented with eight different organic and inorganic phosphate sources aiming at 1.8% phosphorus per dry matter and calcium to phosphorus ratio between 1.3 and 1.7 to 1. Eight healthy beagle dogs (f/m, 2–4 years, 12.9 ± 1.4 kg body weight) were available for the trial. Highly significant differences in the serum kinetics of phosphorus, PTH, and BAP with the highest postprandial levels after feeding highly water-soluble sodium and potassium phosphates were found. We conclude that the use of certain inorganic phosphates in pet food is potentially harmful and should be restricted.
The Relation between Migratory Activity of Pipistrellus Bats at Sea and Weather Conditions Offers Possibilities to Reduce Offshore Wind Farm Effects
Authors: Brabant Robin , Laurent Yves , Jonge Poerink Bob , Degraer Steven
Published: December 2021

Bats undertaking seasonal migration between summer roosts and wintering areas can cross large areas of open sea. Given the known impact of onshore wind turbines on bats, concerns were raised on whether offshore wind farms pose risks to bats. Better comprehension of the phenology and weather conditions of offshore bat migration are considered as research priorities for bat conservation and provide a scientific basis for mitigating the impact of offshore wind turbines on bats. This study investigated the weather conditions linked to the migratory activity of Pipistrellus bats at multiple near- and offshore locations in the Belgian part of the North Sea. We found a positive relationship between migratory activity and ambient temperature and atmospheric pressure and a negative relationship with wind speed. The activity was highest with a wind direction between NE and SE, which may favor offshore migration towards the UK. Further, we found a clear negative relationship between the number of detections and the distance from the coast. At the nearshore survey location, the number of detections was up to 24 times higher compared to the offshore locations. Our results can support mitigation strategies to reduce offshore wind farm effects on bats and offer guidance in the siting process of new offshore wind farms.
Polymorphisms of SORBS1 Gene and Their Correlation with Milk Fat Traits of Cattleyak
Authors: Yang Luyu , Min Xingyu , Zhu Yanjin , Hu Yulei , Yang Manzhen , Yu Hailing , Li Jian , Xiong Xianrong
Published: December 2021

This study aimed to find the SNPs in the SORBS1 gene of cattleyak, analyze the relationship between its polymorphisms and the milk fat traits, and find potential molecular markers for the milk fat traits of cattleyak. The polymorphism of the SORBS1 gene in 350 cattleyak from Hongyuan County (Sichuan, China) were detected by PCR and DNA sequencing, and the correlation between these SNPs and the milk production traits of cattleyak was analyzed. The results showed that there were nine SNPs in the CDS and their adjacent non-coding regions of the SORBS1 gene, and all SNPs have three genotypes. The correlation analysis found that the genotypes with superior milk fat traits in the other eight alleles were homozygous genotypes with a high genotype frequency except the g.96284 G > A (c.3090 G > A) (p < 0.05). However, at locus g.96284 G > A, the milk fat percentage, monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs) and saturated fatty acids (SFAs) of the GA genotype were significantly higher than that of GG and AA genotypes (p < 0.05). Among these SNPs, three SNPs (g.6256 C > T (c.298 C > T), g.24791 A > G (c.706 A > G) and g.29121 A > G (c.979 A > G)) caused the amino acids change. The genotypes of the three SNPs consist of three haplotypes and four diplotypes. The amino acid mutation degree of diplotype H1–H1 (CCAAAA) was the highest, and its milk fat percentage, MUFAs, PUFAs and SFAs were also the highest (p < 0.05). Taken together, we found nine SNPs in the SORBS1 gene that are closely related to the milk fat traits of cattleyak. Moreover, the mutation of amino acids caused by SNPs had positive effects on the milk fat traits of cattleyak. H1-H1 is the dominant diplotype which significantly related to the milk fat traits of cattleyak. This study provides a new molecular marker and theoretical basis for screening the milk fat traits of cattleyak.
Association between Cardiac Auscultation and Echocardiographic Findings in Warmblood Horses
Authors: Hövener Jakob , Pokar Julie , Merle Roswitha , Gehlen Heidrun
Published: December 2021

Heart murmurs are detected frequently when auscultating horses and certain murmurs can usually be linked to specific valvular regurgitations. Limited information exists about the accuracy of these broad rules in warmblood horses and the influence of grade of the regurgitation and dimensional changes on murmur intensity. This study aims to clarify the accuracy of cardiac auscultation in warmblood horses and the influence of the grade of regurgitation and dimensional changes on the loudness of the murmur. In this retrospective study, 822 warmblood horses presented for cardiac examination in a large equine referral center in northern Germany underwent a thorough cardiac auscultation. In total, 653 of these revealed one or more heart murmurs. Most common auscultatory findings were left-sided systolic murmurs (68%) or left-sided diastolic murmurs (15%). On 635 of these horses, an echocardiographic examination was performed, revealing regurgitations of the mitral valve as the most common valvular regurgitation (77%) followed by regurgitations of the aortic valve (23%). Thirty-one percent of horses that underwent echocardiographic examination displayed dimensional changes of one or more compartments of the heart, with the left atrium being most affected (21%), followed by the left ventricle (13%). The main goal of this study was to link certain auscultatory findings with results of the echocardiographic examinations, trying to determine whether auscultation and echocardiography agreed on the valve affected, as well as to find out if loudness of the murmur coincided with grade of regurgitation and presence of dimensional changes. Agreement between auscultation and cardiac ultrasound was substantial (Kappa 0.74) if one or more murmurs and regurgitations were present and almost perfect (Kappa 0.94) if only one murmur and one regurgitation were found. Auscultation was particularly well suited for detection of left-sided systolic and diastolic murmurs, with 87% of left-sided systolic murmurs being caused by a mitral valve regurgitation and 81% of left-sided diastolic murmurs originating from an aortic valve regurgitation. We found a fair agreement between the grade of regurgitation and the respective murmur. Association was particularly good between mild regurgitations and low-grade murmurs, while differentiation between moderate to severe regurgitation based upon the loudness of the murmur was less reliable. Dimensional changes were usually linked to more severe regurgitations and higher-grade murmurs. However, a direct correlation between murmur intensity and the presence or severity of dimensional changes, independent of the grade of valvular regurgitation, could not be established in this cohort of horses.
Beta-Lactam vs. Fluoroquinolone Monotherapy for Pseudomonas aeruginosa Infection: A Systematic Review and Meta-Analysis
Authors: Reid Eric , Walters Ryan W. , Destache Christopher J.
Published: December 2021

Introduction: Pseudomonas aeruginosa (PA) is a leading cause of healthcare-associated infections. A variety of antibiotic classes are used in the treatment of PA infections, including beta-lactams (BLs) and fluoroquinolones (FQs), given either together in combination therapy or alone in monotherapy. A systematic review and meta-analysis were performed to evaluate the therapeutic efficacy of BL agents versus FQ agents as active, definitive monotherapy in PA infections in adults. Methods: Comprehensive literature searches of the Medline and Scopus electronic databases, alongside hand searches of the Cochrane Database of Systematic Reviews, PubMed, and Google Scholar, were performed without a time restriction to identify studies published in English comparing BL and FQ agents given as monotherapy for PA infection in hospitalized adults for which mortality, bacteriological eradication, or clinical response was evaluated. One reviewer screened search results based on pre-defined selection criteria. Two reviewers independently assessed included studies for methodological quality using NIH assessment tools. Two fixed-effects meta-analyses were performed. Results: A total of 368 articles were screened, and six studies involving 338 total patients were included in the meta-analysis. Upon evaluation of methodological quality, two studies were rated good, three fair, and one poor. A meta-analysis of three studies demonstrates FQ monotherapy is associated with significantly improved survival compared to BL monotherapy for patients with PA bacteremia (OR, 3.65; 95% CI, 1.27–10.44; p = 0.02). A meta-analysis of three studies demonstrates FQ monotherapy is associated with equivalent bacteriological eradication compared to BL monotherapy for PA pneumonia or skin and soft tissue infection (RD, 0.07; 95% CI, −0.09 to 0.24; p = 0.39). Conclusion: The meta-analyses demonstrate FQ monotherapy significantly improves survival in PA bacteremia and is associated with similar rates of bacteriological eradication in pneumonia and skin and soft tissue infection caused by PA compared to BL monotherapy. However, more research is needed to make meaningful clinical recommendations.
Heme-Oxygenase-1 Attenuates Oxidative Functions of Antigen Presenting Cells and Promotes Regulatory T Cell Differentiation during Fasciola hepatica Infection
Authors: Costa Monique , da Costa Valeria , Frigerio Sofía , Festari María Florencia , Landeira Mercedes , Rodríguez-Zraquia Santiago A. , Lores Pablo , Carasi Paula , Freire Teresa
Published: December 2021

Fasciola hepatica is a fluke that infects livestock and humans causing fasciolosis, a zoonotic disease of increasing importance due to its worldwide distribution and high economic losses. The parasite regulates the host immune system by inducing a strong Th2 and regulatory T (Treg) cell immune response through mechanisms that might involve the expression or activity of heme-oxygenase-1 (HO-1), the rate-limiting enzyme in the catabolism of free heme that also has immunoregulatory and antioxidant properties. In this paper, we show that F. hepatica-infected mice upregulate HO-1 on peritoneal antigen-presenting cells (APC), which produce decreased levels of both reactive oxygen and nitrogen species (ROS/RNS). The presence of these cells was associated with increased levels of regulatory T cells (Tregs). Blocking the IL-10 receptor (IL-10R) during parasite infection demonstrated that the presence of splenic Tregs and peritoneal APC expressing HO-1 were both dependent on IL-10 activity. Furthermore, IL-10R neutralization as well as pharmacological treatment with the HO-1 inhibitor SnPP protected mice from parasite infection and allowed peritoneal APC to produce significantly higher ROS/RNS levels than those detected in cells from infected control mice. Finally, parasite infection carried out in gp91phox knockout mice with inactive NADPH oxidase was associated with decreased levels of peritoneal HO-1+ cells and splenic Tregs, and partially protected mice from the hepatic damage induced by the parasite, revealing the complexity of the molecular mechanisms involving ROS production that participate in the complex pathology induced by this helminth. Altogether, these results contribute to the elucidation of the immunoregulatory and antioxidant role of HO-1 induced by F. hepatica in the host, providing alternative checkpoints that might control fasciolosis.
Therapeutic Potential of Emerging NAD+-Increasing Strategies for Cardiovascular Diseases
Authors: Rotllan Noemi , Camacho Mercedes , Tondo Mireia , Diarte-Añazco Elena M. G. , Canyelles Marina , Méndez-Lara Karen Alejandra , Benitez Sonia , Alonso Núria , Mauricio Didac , Escolà-Gil Joan Carles , Blanco-Vaca Francisco , Julve Josep
Published: December 2021

Cardiovascular diseases are the leading cause of death worldwide. Aging and/or metabolic stress directly impact the cardiovascular system. Over the last few years, the contributions of altered nicotinamide adenine dinucleotide (NAD+) metabolism to aging and other pathological conditions closely related to cardiovascular diseases have been intensively investigated. NAD+ bioavailability decreases with age and cardiometabolic conditions in several mammalian tissues. Compelling data suggest that declining tissue NAD+ is commonly related to mitochondrial dysfunction and might be considered as a therapeutic target. Thus, NAD+ replenishment by either genetic or natural dietary NAD+-increasing strategies has been recently demonstrated to be effective for improving the pathophysiology of cardiac and vascular health in different experimental models, as well as human health, to a lesser extent. Here, we review and discuss recent experimental evidence illustrating that increasing NAD+ bioavailability, particularly by the use of natural NAD+ precursors, may offer hope for new therapeutic strategies to prevent and treat cardiovascular diseases.
mTOR Inhibition via Rapamycin Treatment Partially Reverts the Deficit in Energy Metabolism Caused by FH Loss in RPE Cells
Authors: Merle David A. , Provenzano Francesca , Jarboui Mohamed Ali , Kilger Ellen , Clark Simon J. , Deleidi Michela , Armento Angela , Ueffing Marius
Published: December 2021

Age-related macular degeneration (AMD) is a complex degenerative disease of the retina with multiple risk-modifying factors, including aging, genetics, and lifestyle choices. The combination of these factors leads to oxidative stress, inflammation, and metabolic failure in the retinal pigment epithelium (RPE) with subsequent degeneration of photoreceptors in the retina. The alternative complement pathway is tightly linked to AMD. In particular, the genetic variant in the complement factor H gene (CFH), which leads to the Y402H polymorphism in the factor H protein (FH), confers the second highest risk for the development and progression of AMD. Although the association between the FH Y402H variant and increased complement system activation is known, recent studies have uncovered novel FH functions not tied to this activity and highlighted functional relevance for intracellular FH. In our previous studies, we show that loss of CFH expression in RPE cells causes profound disturbances in cellular metabolism, increases the vulnerability towards oxidative stress, and modulates the activation of pro-inflammatory signaling pathways, most importantly the NF-kB pathway. Here, we silenced CFH in hTERT-RPE1 cells to investigate the mechanism by which intracellular FH regulates RPE cell homeostasis. We found that silencing of CFH results in hyperactivation of mTOR signaling along with decreased mitochondrial respiration and that mTOR inhibition via rapamycin can partially rescue these metabolic defects. To obtain mechanistic insight into the function of intracellular FH in hTERT-RPE1 cells, we analyzed the interactome of FH via immunoprecipitation followed by mass spectrometry-based analysis. We found that FH interacts with essential components of the ubiquitin-proteasomal pathway (UPS) as well as with factors associated with RB1/E2F signalling in a complement-pathway independent manner. Moreover, we found that FH silencing affects mRNA levels of the E3 Ubiquitin-Protein Ligase Parkin and PTEN induced putative kinase (Pink1), both of which are associated with UPS. As inhibition of mTORC1 was previously shown to result in increased overall protein degradation via UPS and as FH mRNA and protein levels were shown to be affected by inhibition of UPS, our data stress a potential regulatory link between endogenous FH activity and the UPS.
The Relationship between Ozone and Human Blood in the Course of a Well-Controlled, Mild, and Transitory Oxidative Eustress
Authors: Tricarico Gerardo , Travagli Valter
Published: December 2021

In the last twenty years there has been a proliferation of articles on the therapeutic use of ozone. As it is well-known, the term ozone therapy is very broad. It ranges from either systemic or loco-regional administration of unstable gaseous oxygen/ozone mixtures to the topical application of stable ozonated derivatives. Anyway, in relation to the absence of specific receptors and the extreme reactivity with the biological liquids with which it comes into contact, gaseous ozone cannot be classified as either a drug or a pro-drug. When the gaseous ozone impacts a biological matrix, both reactive oxygen species (ROS) and lipid oxidation products (LOPs) are formed. They represent the effector molecules responsible for modulating the therapeutic activity in the body. Apart from the merits of the action mechanisms resulting from the use of ozone, this article seeks to validate the practice of ozone therapy as an adjuvant treatment in full compliance with the physiology of the whole organism.