bluuebunny/crossref_metadata_2025 huggingface dataset:
(S)-2-(Cyclobutylamino)-N-(3-(3,4-dihydroisoquinolin-2(1H)-yl)-2-hydroxypropyl)isonicotinamide Attenuates RANKL-Induced Osteoclast Differentiation by Inhibiting NF-κB Nuclear Translocation
Authors: Ding Mina , Cho Eunjin , Chen Zhihao , Park Sang-Wook , Lee Tae-Hoon
Published: February 2023

Osteoporosis is a common skeletal disease; however, effective pharmacological treatments still need to be discovered. This study aimed to identify new drug candidates for the treatment of osteoporosis. Here, we investigated the effect of EPZ compounds, protein arginine methyltransferase 5 (PRMT5) inhibitors, on RANKL-induced osteoclast differentiation via molecular mechanisms by in vitro experiments. EPZ015866 attenuated RANKL-induced osteoclast differentiation, and its inhibitory effect was more significant than EPZ015666. EPZ015866 suppressed the F-actin ring formation and bone resorption during osteoclastogenesis. In addition, EPZ015866 significantly decreased the protein expression of Cathepsin K, NFATc1, and PU.1 compared with the EPZ015666 group. Both EPZ compounds inhibited the nuclear translocation of NF-κB by inhibiting the dimethylation of the p65 subunit, which eventually prevented osteoclast differentiation and bone resorption. Hence, EPZ015866 may be a potential drug candidate for the treatment of osteoporosis.
Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
Authors: Santoro Orlando , Malacarne Miryam Chiara , Sarcone Francesco , Scapinello Luca , Pragliola Stefania , Caruso Enrico , Orlandi Viviana Teresa , Izzo Lorella
Published: February 2023

In this work, two compounds belonging to the BODIPY family, and previously investigated for their photosensitizing properties, have been bound to the amino-pendant groups of three random copolymers, with different amounts of methyl methacrylate (MMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) in the backbone. The P(MMA-ran-DMAEMA) copolymers have inherently bactericidal activity, due to the amino groups of DMAEMA and to the quaternized nitrogens bounded to BODIPY. Systems consisting of filter paper discs coated with copolymers conjugated to BODIPY were tested on two model microorganisms, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). On solid medium, irradiation with green light induced an antimicrobial effect, visible as a clear inhibition area around the coated disks. The system based on the copolymer with 43% DMAEMA and circa 0.70 wt/wt% of BODIPY was the most efficient in both bacterial species, and a selectivity for the Gram-positive model was observed, independently of the conjugated BODIPY. A residual antimicrobial activity was also observed after dark incubation, attributed to the inherently bactericidal properties of copolymers.
Processing of Phosphoric Solid Waste by Humic Acid Leaching Method
Authors: Nazarbek Ulzhalgas , Abdurazova Perizat , Nazarbekova Saule , Raiymbekov Yerkebulan , Kambatyrov Maksat
Published: February 2023

This article presents the results of research on the leaching of solid phosphorus-containing waste with humic acid. Such waste includes the by-products of the electrothermal processing of phosphate raw materials—phosphorus sludge and cottrel dust. Chemical and X-ray diffraction analyses have been used to study their composition and phase structure, according to which these substances have an amorphous structure. The leaching of phosphoric sludge and cottrel dust was investigated by varying the main parameters. The obtained data were processed using the method of formal kinetics to study the features of the process. The reaction rate constants and the apparent activation energy were calculated, and the values found made it possible to determine that the process under study is limited by diffusion. The scientific novelty of the article is the use of humic acid for leaching phosphoric solid waste as opposed to traditional methods. This new method may offer improved efficiency, reduced environmental impact, and a potential alternative solution for the processing of phosphoric waste.
Percutaneous Coronary Intervention Outcomes in Patients with Liver Cirrhosis: A Systematic Review and Meta-Analysis
Authors: Khandait Harshwardhan , Jaiswal Vikash , Hanif Muhammad , Shrestha Abhigan Babu , Iturburu Alisson , Shah Maitri , Ishak Angela , Garimella Vamsi , Ang Song Peng , Mathew Midhun
Published: February 2023

There is a paucity of data and minimal literature on outcomes of percutaneous coronary intervention (PCI) among liver cirrhosis patients. Therefore, we conducted a systematic review and meta-analysis to evaluate the clinical outcomes among liver cirrhosis patients post-PCI. We conducted a comprehensive literature search in the PubMed, Embase, Cochrane, and Scopus databases for relevant studies. Effect sizes were pooled using the DerSimonian and Laird random-effects model as an odds ratio (OR) with 95% confidence intervals (CI). A total of 3 studies met the inclusion criteria, providing data from 10,705,976 patients. A total of 28,100 patients were in the PCI + Cirrhosis group and 10,677,876 patients were in the PCI-only group. The mean age of patients with PCI + Cirrhosis and PCI alone was 63.45 and 64.35 years. The most common comorbidity was hypertension among the PCI + Cirrhosis group compared with PCI alone (68.15% vs. 73.6%). Cirrhosis patients post-PCI were had higher rates of in-hospital mortality (OR, 4.78 (95%CI: 3.39–6.75), p < 0.001), GI bleeding (OR, 1.91 (95%CI:1.83–1.99), p < 0.001, I2 = 0%), stroke (OR, 2.48 (95%CI:1.68–3.66), p < 0.001), AKI (OR, 3.66 (95%CI: 2.33–6.02), p < 0.001), and vascular complications (OR, 1.50 (95%CI: 1.13–1.98), p < 0.001) compared with the PCI group without cirrhosis. Patients with cirrhosis are at a high risk for mortality and adverse outcomes post-PCI procedure compared to the PCI-only group of patients.
William Stern: The Relevance of His Program of ‘Differential Psychology’ for Contemporary Intelligence Measurement and Research
Authors: Kovacs Kristof , Pléh Csaba
Published: February 2023

William Stern is mostly renowned for inventing the IQ formula. However, he is also the originator of the term ‘differential psychology’ itself. His program of differential psychology synthesized population-based correlational studies as well as idiosyncratic approaches focusing on unique profiles of individuals. We argue that his approach still offers valuable ideas to this day; in particular, the individualistic sub-programme of Stern’s differential psychology corresponds to a large extent to ipsative testing that emphasizes a profile-based analysis of individual strengths and weaknesses.
Initial Design of a Novel Barge-Type Floating Offshore Wind Turbine in Shallow Water
Authors: Zhou Yiming , Feng Sensen , Guo Xiaojiang , Tian Feng , Han Xu , Shi Wei , Li Xin
Published: February 2023

The studies on floating offshore wind turbines (FOWTs) have been increasing over recent decades due to the growing interest in offshore renewable energy. The present paper proposes a barge platform with four moonpools to support the Technical University of Denmark 10 MW wind turbine for a designed water depth of 60 m. A 4 × 2 mooring system with eight mooring lines is also proposed for the barge platform. The main dimensions of the barge platform are optimally selected with respect to its preliminary hydrodynamic properties and potential financial benefit. The proposed barge-type FOWT is then demonstrated to be aligned with the DNV standard requirements in terms of its intact and damage stability. Furthermore, coupled time-domain simulations are conducted for the proposed barge FOWT with mooring under the selected environmental and operational conditions by using Simo-Riflex-AeroDyn (SRA). Through decay test simulations, the natural periods of the barge-type FOWT are demonstrated to be within the DNV recommended ranges. The proposed mooring system is also benchmarked with the 3 × 3 mooring concept that was used for a 3 MW barge-type FOWT installed in Kitakyushu. The response magnitudes of the barge platform and mooring line tension are similar to both mooring systems, and thus the 4 × 2 mooring system is preferred due to its lower cost. In addition, the proposed barge platform is preliminarily demonstrated to be able to survive for the 50-year extreme environmental conditions under parked wind turbine status, as well as the normal environmental conditions under the operating status.
Feature Recognition on Friction Induced Vibration of Water-Lubricated Bearing under Low Speed and Heavy Load
Authors: Jin Yong , Li Ruiqing , Ouyang Wu , Liu Zhenglin , Liu Qilin , Xiong Qipeng , Zhou Jianhui
Published: February 2023

Under low speed and heavy load operating conditions, the marine water-lubricated bearing (WLB) is often in a mixed lubrication state. This leads to abnormal friction-induced vibrations and noise in underwater vehicles. An empirical mode decomposition (EMD) based method for identifying friction-induced vibration characteristics of WLB was proposed, and friction-induced vibration experiments of WLB were carried out. The results showed that the specific pressure and lubricant temperature significantly affected friction-induced vibrations while the cooling water flow hardly had an effect. Studying the monotonicity of the eigenvalues of IMF energy, time-domain standard deviation, bias angle, and center of gravity frequency can visualize the trend of the bearing friction-induced vibration signals. Among them, the critical point of friction-induced vibrations could be analyzed by the inflection point of IMF energy, standard deviation, bias angle, and the sudden increase of the center of gravity frequency. The study results were valuable for revealing the friction-induced vibration mechanism of WLB and providing an important reference for design optimization.
Marine Policy
Authors: Lau Yui-yip , Kawasaki Tomoya
Published: February 2023

The volume of international maritime transport is continuously increasing due to worldwide economic growth and the sophistication of the global supply chain [...]
Morphology, Molecular Genetics and Potential Importance for Mucilage Events of the New Coccolithophorid Ochrosphaera neapolitana in the Sea of Marmara
Authors: Eker-Develi Elif , Tekdal Dilek , Demet Atıf , Yıldız Hüseyin , Kideys Ahmet
Published: February 2023

The coccolithophorid Ochrosphaera neapolitana was reported for the first time from samples obtained during a large-scale mucilage event in the Sea of Marmara in May 2022 in a previous study. We also found this species in our samples obtained about a year ago (i.e., in June 2021). In our study, O. neapolitana was further isolated and produced in the laboratory as a monoculture for further investigations using electron microscopy and molecular methods. Ochrosphaera neapolitana was identified using a small sub-unit (SSU) rRNA sequence and subsequent phylogenetic analysis. During the laboratory experiments, O. neapolitana was surprisingly observed to produce conspicuous levels of mucilage as a skim layer in mono- or multi-species cultures, mainly comprising other diatom species. This observation could be a significant milestone in understanding the reasons and mechanisms of mucilage events that occur in the Sea of Marmara.
Mycotoxins from Tomato Pathogenic Alternaria alternata and Their Combined Cytotoxic Effects on Human Cell Lines and Male Albino Rats
Authors: Ismail Ahmed , Elshewy Eman , El-Ganainy Sherif , Magistà Donato , Hamouda Ahlam , Alhudaib Khalid , Ebrahim Weaam , Almaghasla Mustafa
Published: February 2023

The Alternaria species are considered to produce a plethora of several mycotoxins constituting a risk factor for both human and animal health. This work aimed mainly to explore the cytotoxicity of a combined mixture of altenuene (ALT), alternariol (AOH), tenuazonic acid (TeA), and altenuisol (AS) toxins produced by pathogenic A. alternata toward human oral epithelial cells (PCS-200-014), lung fibroblast cells (WI-38), and male albino rats. The sequencing of the multi-locus, RNA polymerase second largest subunit (rpb2), glyceraldehyde-3-phosphate dehydrogenase (gapdh), and Alternaria major allergen gene (Alt a 1) was performed to infer relationships among isolated Alternaria species. The phylogenetic analysis of gapdh, rpb2, and Alt-a 1 sequence data indicated that all isolates resided in A. alternata. The pathogenic potentiality of A. alternata was investigated on tomato plants cv. super strain B under greenhouse conditions, and all isolates were pathogenic to tomato plants, with significant (p < 0.05) variations. The ability of A. alternata isolates to produce mycotoxins was also explored using high-performance liquid chromatography (HPLC). All tested isolates were able to produce at least one of the assessed mycotoxins—ALT, AOH, TeA, and AS—and ALT was reported as the dominant mycotoxin, produced by 80% of A. alternata isolates. The cytotoxic properties of the combined mixture of ALT, AOH, TeA, and AS at concentrations of 31.25, 62.50, 125, 250, and 500 µg/mL were assessed via the MTT assay method after exposure for 24 h versus the control. The treatment of both cell lines with combined mixtures of ALT, AOH, TeA, and AS showed a dose-dependent decrease in cell viability. The highest concentrations tested at 62.50, 125, 250, and 500 µg/mL significantly decreased cell viability and caused cell damage compared to the lowest concentration of 31.25 µg/mL and the control. The cytotoxicity and genotoxicity of the combined mixtures of ALT, AOH, TeA, and AS on male albino rats were also investigated via the gene expression of (TNF-α) and using hematological (CBC), chemical (alanine aminotransferase (ALT), aspartate aminotransferase (AST) and urea and creatinine), and histopathological analyses. A marked increase was observed in the levels of ALT, AST, urea and creatinine, TNF-α gene expression, red blood cells (RBCs), white blood cells (WBCs), hemoglobin (Hb), and packed cell volume % (PCV) after 28 days of exposure relative to the untreated control. Pathological alterations were also observed in the liver and kidney tissues of rats. Conclusively, this work provides a new understanding on the cytotoxicity and genotoxicity of mycotoxins of pathogenic A. alternata from tomatoes.
Phaeohyphomycosis in Solid Organ Transplant Recipients: A Case Series and Narrative Review of the Literature
Authors: Lo Porto Davide , Cona Andrea , Todaro Francesca , De Carolis Elena , Cardinale Francesca , Hafeez Neha , Di Martino Giuseppina , Conaldi Pier Giulio , Sanguinetti Maurizio , Grossi Paolo Antonio , Mularoni Alessandra
Published: February 2023

Phaeohyphomycosis comprises a variety of infections caused by pigmented fungi. Solid organ transplant (SOT) recipients are particularly at risk of invasive infections due to their prolonged immunosuppression. Here, we describe three cases of phaeohyphomycosis in SOT recipients who were successfully treated with surgical excision and/or antifungal therapy. We additionally carried out a narrative review of the literature on phaeohyphomycosis in 94 SOT recipients from 66 published studies describing 40 different species of fungi. The most reported fungus was Alternaria (21%). The median time from transplant to diagnosis was 18 months (IQR 8.25–48), and kidney transplants were the most reported. Antifungal regimens were not homogeneous, though there was a prevalence of itraconazole- and voriconazole-based treatments. Clinical outcomes included recovery in 81% and death in 5% of infected SOT recipients. Susceptibility testing was done in 26.6% of the cases, with heterogeneous results due to the variety of species isolated. While the wide diversity of dematiaceous fungi and their host range make it difficult to offer a uniform approach for phaeohyphomycosis, an early diagnosis and therapy are critical in preventing the dissemination of disease in the immunocompromised host.
Structural Analysis of the A Mating Type Locus and Development of the Mating Type Marker of Agaricus bisporus var. bisporus
Authors: Choi Yeon-Jae , Jung Sujin , Eom Hyerang , Hoang Thimen , Han Hui-Gang , Kim Sinil , Ro Hyeon-Su
Published: February 2023

Karyotyping in Agaricus bisporus is crucial for both the isolation of homokaryotic strains and the confirmation of dikaryon establishment. For the verification of the karyotype, the A mating type loci of two homokaryotic strains, H39 and H97, were analyzed through comparative sequence analysis. The two loci showed major differences in two sequence regions designated as Region 1 and Region 2. H97 had a putative DNA transposon in Region 1 that had target site duplications (TSDs), terminal inverted repeats (TIRs), and a loop sequence, in contrast to H39, which only had the insertional target sequence. Homologous sequences of the transposon were discovered in the two different chromosomes of H97 and in one of H39, all of which have different TSDs but share high sequence homology in TIR. Region 2 shared three consensus sequences between H97 and H39. However, it was only from H97 that a large insertional sequence of unknown origin was discovered between the first and second consensus sequences. The difference in length in Region 1, employed for the verification of the A mating type, resulted in the successful verification of mating types in the heterokaryotic and homokaryotic strains. This length difference enables the discrimination between homo- and heterokaryotic spores by PCR. The present study suggests that the A mating type locus in A. bisporus H97 has evolved through transposon insertion, allowing the discrimination of the mating type, and thus the nuclear type, between A. bisporus H97 and H39.
Escherichia coli, but Not Staphylococcus aureus, Functions as a Chelating Agent That Exhibits Antifungal Activity against the Pathogenic Yeast Candida albicans
Authors: Bose Swagata , Singh Durg Vijai , Adhya Tapan Kumar , Acharya Narottam
Published: February 2023

Humans are colonized by diverse populations of microbes. Infections by Candida albicans, an opportunistic fungal pathogen, are a result of imbalances in the gut microbial ecosystem and are due to the suppressed immunity of the host. Here, we explored the potential effects of the polymicrobial interactions of C. albicans with Staphylococcus aureus, a Gram-positive bacterium, and Escherichia coli, a Gram-negative bacterium, in dual and triple in vitro culture systems on their respective growth, morphology, and biofilms. We found that S. aureus promoted the fungal growth and hyphal transition of C. albicans through cell-to-cell contacts; contrarily, both the cell and cell-free culture filtrate of E. coli inhibited fungal growth. A yet to be identified secretory metabolite of E. coli functionally mimicked EDTA and EGTA to exhibit antifungal activity. These findings suggested that E. coli, but not S. aureus, functions as a chelating agent and that E. coli plays a dominant role in regulating excessive growth and, potentially, the commensalism of C. albicans. Using animal models of systemic candidiasis, we found that the E. coli cell-free filtrate suppressed the virulence of C. albicans. In general, this study unraveled a significant antimicrobial activity and a potential role in the nutritional immunity of E. coli, and further determining the underlying processes behind the E. coli–C. albicans interaction could provide critical information in understanding the pathogenicity of C. albicans.
Urban Commons between Ostrom’s and Neo-Materialist Approaches: The Case of Lido Pola in Naples, Southern Italy
Authors: Vittoria Maria Patrizia , Ragozino Stefania , Esposito De Vita Gabriella
Published: February 2023

The main aim of the study was reflecting on performative implications of the urban commons and their relational ability (i.e., inter- and/or intra-actioning) within an inclusive governance model and policy design context through two interpretative keys: Ostrom’s idea of sustainability and the recent hybrid neo-materialist urban and organizational theoretical path grounded within the Metzger–Barad–Latour analyses. Firstly, we focused on defining the theoretical setting, background and selected codes. The resulting scheme was tested with a mixed methodology within the case study of the Lido Pola Commons in Naples, Southern Italy. Empirical analysis benefits from long-lasting research experience on the area and an action-research processes aimed at codesigning a living civic lab. The discussion illustrates the main pivots of the internal/external validation of the case study results, thus contributing to enhancing a participatory policy design by raising awareness regarding social intra/interactions.
Analysis on Controllability Results for Impulsive Neutral Hilfer Fractional Differential Equations with Nonlocal Conditions
Authors: Linitda Thitiporn , Karthikeyan Kulandhaivel , Sekar Palanisamy Raja , Sitthiwirattham Thanin
Published: February 2023

In this paper, we investigate the controllability of the system with non-local conditions. The existence of a mild solution is established. We obtain the results by using resolvent operators functions, the Hausdorff measure of non-compactness, and Monch’s fixed point theorem. We also present an example, in order to elucidate one of the results discussed.
Formal Verification of a Topological Spatial Relations Model for Geographic Information Systems in Coq
Authors: Yan Sheng , Yu Wensheng
Published: February 2023

Geographic information systems have undergone rapid growth for decades. Topology has provided valuable modeling tools in the development of this field. Formal verification ofthe model of topological spatial relations can provide a reliable guarantee for the correctness of geographic information systems. We present a proof of the topological spatial relations model that has been formally verified in the Coq proof assistant. After an introduction to the formalization of the axiomatic set theory of Morse–Kelley, the formal description of the elementary concepts and properties of general topology is developed. The topological spatial relations between two sets are described by using the concept of the intersection value. Finally, we formally proved the topological spatial relations between two sets which are restricted to the regularly closed and the planar spatial regions. All the proof details are strictly completed in Coq, which shows that the correctness of the theoretical model for geographic information systems can be checked by a computer. This paper provides a novel method to verify the correctness of the topological spatial relations model. This work can also contribute to the creation and validation of various geological models and software.
A Novel EM-Type Algorithm to Estimate Semi-Parametric Mixtures of Partially Linear Models
Authors: Skhosana Sphiwe B. , Millard Salomon M. , Kanfer Frans H. J.
Published: February 2023

Semi- and non-parametric mixture of normal regression models are a flexible class of mixture of regression models. These models assume that the component mixing proportions, regression functions and/or variances are non-parametric functions of the covariates. Among this class of models, the semi-parametric mixture of partially linear models (SPMPLMs) combine the desirable interpretability of a parametric model and the flexibility of a non-parametric model. However, local-likelihood estimation of the non-parametric term poses a computational challenge. Traditional EM optimisation of the local-likelihood functions is not appropriate due to the label-switching problem. Separately applying the EM algorithm on each local-likelihood function will likely result in non-smooth function estimates. This is because the local responsibilities calculated at the E-step of each local EM are not guaranteed to be aligned. To prevent this, the EM algorithm must be modified so that the same (global) responsibilities are used at each local M-step. In this paper, we propose a one-step backfitting EM-type algorithm to estimate the SPMPLMs and effectively address the label-switching problem. The proposed algorithm estimates the non-parametric term using each set of local responsibilities in turn and then incorporates a smoothing step to obtain the smoothest estimate. In addition, to reduce the computational burden imposed by the use of the partial-residuals estimator of the parametric term, we propose a plug-in estimator. The performance and practical usefulness of the proposed methods was tested using a simulated dataset and two real datasets, respectively. Our finite sample analysis revealed that the proposed methods are effective at solving the label-switching problem and producing reasonable and interpretable results in a reasonable amount of time.
Morphological, Physiological and Mechanical Features of the Mutable Collagenous Tissues Associated with Autotomy and Evisceration in Dendrochirotid Holothuroids
Authors: Byrne Maria
Published: February 2023

Evisceration in dendrochirotid sea cucumbers leads to expulsion of the digestive tract, pharyrngeal complex and coelomic fluid through rupture of the anterior body wall. This process involves failure of three mutable collagenous tissue (MCT) structures, the introvert, the pharyngeal retractor muscle tendon, and the intestine-cloacal junction. These are complex structures composed of several tissue strata. The MCT in the three autotomy structures contains collagen fibrils, unstriated microfibrils, and interfibrillar molecules. Neurosecretory-like processes (juxtaligamental-type) with large dense vesicles (LDVs) are prominent in the autotomy structures. Biomechanical tests show that these structures are not inherently weak. Failure of the autotomy structures can be elicited by manipulating the ionic environment and the changes are blocked by anaesthetics. Autotomy and evisceration are under neural control, but local neural elements and neurosecretory-like processes do not appear to be a source of factors that cause MCT destabilisation. The LDVs remain intact while the tissue destabilises. The coelomic fluid contains an evisceration inducing factor indicating a neurosecretory-like mediation of autotomy. This factor elicits muscle contraction and MCT destabilisation. As the autotomy structures are completely or partially surrounded by coelomic fluid, the agent(s) of change may be located in the coelom (systemic origin) as well as originate from cells within the MCT. The biochemistry and mechanism(s) of action of the evisceration factor are not known. This factor is a promising candidate for biodiscovery investigation.
Bioactive Oxylipins Profile in Marine Microalgae
Authors: Linares-Maurizi Amandyne , Reversat Guillaume , Awad Rana , Bultel-Poncé Valérie , Oger Camille , Galano Jean-Marie , Balas Laurence , Durbec Anaelle , Bertrand-Michel Justine , Durand Thierry , Pradelles Rémi , Vigor Claire
Published: February 2023

Microalgae are photosynthetic microscopic organisms that serve as the primary food source in aquatic environments. Microalgae can synthesize a wide variety of molecules, such as polyunsaturated fatty acids (PUFAs) of the omega-3 and omega-6 series. Oxidative degradation of PUFA due to radical and/or enzymatic conversion leads to the formation of oxylipins, which are compounds known for their bioactive properties. In the present study, we aim to profile oxylipins from five microalgae species grown in 10-L photo-bioreactors under optimal conditions. During their exponential phase, microalgae were harvested, extracted and analyzed by LC-MS/MS to determine the qualitative and quantitative profile of oxylipins for each species. The five different selected microalgae revealed a high diversity of metabolites, up to 33 non-enzymatic and 24 enzymatic oxylipins present in different concentrations. Taken together, these findings highlight an interesting role of marine microalgae as a source of bioactive lipids mediators, which we hypothesize have an important function in preventive health measures such as amelioration of inflammation. The rich mixture of oxylipins may display advantages to biological organisms, especially by providing for human health benefits including antioxidant, anti-inflammatory, neuroprotective or immunomodulator activities. Some oxylipins are also well known for their cardiovascular properties.
Morphological and Physiological Aspects of Mutable Collagenous Tissue at the Autotomy Plane of the Starfish Asterias rubens L. (Echinodermata, Asteroidea): An Echinoderm Paradigm
Authors: Wilkie Iain C. , Candia Carnevali M. Daniela
Published: February 2023

The mutable collagenous tissue (MCT) of echinoderms has the capacity to undergo changes in its tensile properties within a timescale of seconds under the control of the nervous system. All echinoderm autotomy (defensive self-detachment) mechanisms depend on the extreme destabilisation of mutable collagenous structures at the plane of separation. This review illustrates the role of MCT in autotomy by bringing together previously published and new information on the basal arm autotomy plane of the starfish Asterias rubens L. It focuses on the MCT components of breakage zones in the dorsolateral and ambulacral regions of the body wall, and details data on their structural organisation and physiology. Information is also provided on the extrinsic stomach retractor apparatus whose involvement in autotomy has not been previously recognised. We show that the arm autotomy plane of A. rubens is a tractable model system for addressing outstanding problems in MCT biology. It is amenable to in vitro pharmacological investigations using isolated preparations and provides an opportunity for the application of comparative proteomic analysis and other “-omics” methods which are aimed at the molecular profiling of different mechanical states and characterising effector cell functions.
The microRNA Cargo of Human Vaginal Extracellular Vesicles Differentiates Parasitic and Pathobiont Infections from Colonization by Homeostatic Bacteria
Authors: Cezar-de-Mello Paula Fernandes Tavares , Ryan Stanthia , Fichorova Raina N.
Published: February 2023

The disturbed vaginal microbiome defined as bacterial vaginosis (BV) and the parasitic infection by Trichomonas vaginalis (TV), the most common non-viral sexually transmitted pathogen, have well-established adverse effects on reproductive outcomes and susceptibility to infection and cancer. Molecular mechanisms underlying these associations and the failure of antibiotic therapy to mitigate adverse consequences are not fully elucidated. In an in vitro human vaginal colonization model, we tested the hypothesis that responses to TV and/or BV-bacteria will disrupt the micro(mi)RNA cargo of extracellular vesicles (EV) with the potential to modify pathways associated with reproductive function, cancer, and infection. miRNAs were quantified by HTG EdgeSeq. MiRNA differential expression (DE) was established in response to TV, the BV signature pathobiont Prevotella bivia and a homeostatic Lactobacillus crispatus with adjusted p < 0.05 using R. Validated gene targets, pathways, protein-protein interaction networks, and hub genes were identified by miRWalk, STRING, Cytoscape, and CytoHubba. In contrast to L. crispatus, TV and the BV pathobiont dysregulated a massive number of EV-miRNAs, over 50% shared by both pathogens. Corresponding target pathways, protein interaction clusters and top hub genes were related to cancer, infectious disease, circadian rhythm, steroid hormone signaling, pregnancy, and reproductive tissue terms. These data support the emerging concept that bacteria and parasitic eukaryotes disturbing the human vaginal microbiome may impact reproductive health through EV-miRNA dysregulation.
Surfactant Intercalation in Li-Al-Based Binary and Ternary Layered Double Hydroxides by the Microwave-Assisted Rapid Ion-Exchange Process and Its Application in Iodine Adsorption
Authors: Yadav Dileep Kumar , Uma Sitharaman , Nagarajan Rajamani
Published: February 2023

Recognizing the extreme speeds of reactions with microwaves, anionic forms of surfactants (sodium dodecyl sulfate (SDS) and sodium dodecylbenzenesulfonate (SDBS)) have been intercalated successfully by ion-exchange reactions in binary Li-Al and ternary Li-M-Al (M = Mg, Co, Ni, Cu, and Zn) layered double hydroxide (LDH) systems with the aid of microwaves. The samples have been characterized extensively. The basal spacings of 28.2 and 30.4 Å have been estimated for Li-Al-DS and Li-Al-DBS LDH samples, respectively, suggesting a perpendicular arrangement of DS− and DBS− anions in the interlayer space. The characteristic vibration bands of both LDH and the surfactant (DS− and DBS−) in the FTIR spectra confirmed the binding mode of surfactant molecules within the interlayers. DS−-intercalated Li-Al LDH showed lower thermal stability than the DBS−-intercalated sample. The nitrate-intercalated Li-M-Al (M = Mg, Co, Ni, Cu, and Zn) LDHs were ion-exchanged with SDS and SDBS to yield DS−-and DBS−-intercalated systems. The expanded basal spacings and a change in crystallite morphology confirmed the vertical intercalation of DS− and DBS− in Li-M-Al LDHs. ICP-AES and elemental analyses determined the metal contents and the surfactant content. FTIR spectra of intercalated samples confirmed the surfactant’s presence in the interlayer. The presence of Co, Ni, and Cu in Li-M-Al LDHs has been confirmed from UV-visible spectra. The Li-Al-DBS sample adsorbed iodine efficiently from methanol solutions, and the Langmuir model could explain the adsorption data in a better way. The adsorption followed pseudo-second-order kinetics.
Toxicity and Physiological Effects of Nine Lamiaceae Essential Oils and Their Major Compounds on Reticulitermes dabieshanensis
Authors: Yang Xi , Jin Chunzhe , Wu Ziwei , Han Hui , Zhang Zhilin , Xie Yongjian , Zhang Dayu
Published: February 2023

The volatile metabolites of Salvia sclarea, Rosmarinus officinalis, Thymus serpyllum, Mentha spicata, Melissa officinalis, Origanum majorana, Mentha piperita, Ocimum basilicum and Lavandula angustifolia were determined by gas chromatography–mass spectrometry. The vapor insecticidal properties of the analyzed essential oils and their compounds were screened using Reticulitermes dabieshanensis workers. The most effective oils were S. sclarea (major constituent linalyl acetate, 65.93%), R. officinalis (1,8-cineole, 45.56%), T. serpyllum (thymol, 33.59%), M. spicata (carvone, 58.68%), M. officinalis (citronellal, 36.99%), O. majorana (1,8-cineole, 62.29%), M. piperita (menthol, 46.04%), O. basilicum (eugenol, 71.08%) and L. angustifolia (linalool, 39.58%), which exhibited LC50 values ranging from 0.036 to 1.670 μL/L. The lowest LC50 values were recorded for eugenol (0.060 μL/L), followed by thymol (0.062 μL/L), carvone (0.074 μL/L), menthol (0.242 μL/L), linalool (0.250 μL/L), citronellal (0.330 μL/L), linalyl acetate (0.712 μL/L) and 1,8-cineole (1.478 μL/L). The increased activity of esterases (ESTs) and glutathione S-transferase (GST) were observed but only alongside the decreased activity of acetylcholinesterase (AChE) in eight main components. Our results indicate that S. sclarea, R. officinalis, T. serpyllum, M. spicata, M. officinalis, O. marjorana, M. piperita, O. basilicum and L. angustifolia essential oils (EOs) and their compounds, linalyl acetate, 1,8-cineole, thymol, carvone, citronellal, menthol, eugenol and linalool could be developed as control agents against termites.
Analysis of Drugs in Saliva of US Military Veterans Treated for Substance Use Disorders Using Supported Liquid Extraction and Surface-Enhanced Raman Spectral Analysis
Authors: Farquharson Stuart , Shende Chetan , Newcomb Jenelle , Petrakis Ismene L. , Arias Albert J.
Published: February 2023

According to the Center for Disease Control, there were more than 107,000 US drug overdose deaths in 2021, over 80,000 of which due to opioids. One of the more vulnerable populations is US military veterans. Nearly 250,000 military veterans suffer from substance-related disorders (SRD). For those seeking treatment, buprenorphine is prescribed to help treat opioid use disorder (OUD). Urinalysis is currently used to monitor buprenorphine adherence as well as to detect illicit drug use during treatment. Sometimes sample tampering occurs if patients seek to generate a false positive buprenorphine urine test or mask illicit drugs, both of which can compromise treatment. To address this problem, we have been developing a point-of-care (POC) analyzer that can rapidly measure both medications used for treatment and illicit drugs in patient saliva, ideally in the physi-cian’s office. The two-step analyzer employs (1) supported liquid extraction (SLE) to isolate the drugs from the saliva and (2) surface-enhanced Raman spectroscopy (SERS) to detect the drugs. A prototype SLE-SERS-POC analyzer was used to quantify buprenorphine at ng/mL concentrations and identify illicit drugs in less than 1 mL of saliva collected from 20 SRD veterans in less than 20 min. It correctly detected buprenorphine in 19 of 20 samples (18 true positives, 1 true negative and 1 false negative). It also identified 10 other drugs in patient samples: acetaminophen, amphetamine, cannabidiol, cocaethylene, codeine, ibuprofen, methamphetamine, methadone, nicotine, and norbuprenorphine. The prototype analyzer shows evidence of accuracy in measuring treatment medications and relapse to drug use. Further study and development of the system is warranted.
Structurally-New Hexadecanuclear Ni-Containing Silicotungstate with Catalytic Hydrogen Generation Activity
Authors: Wang Yequn , Xin Xing , Feng Yeqin , Chi Manzhou , Wang Ruijie , Liu Tianfu , Lv Hongjin
Published: February 2023

A structurally-new, carbon-free hexadecanuclear Ni-containing silicotungstate, [Ni16(H2O)15(OH)9(PO4)4(SiW9O34)3]19-, has been facilely synthesized using a one-pot, solution-based synthetic method systematically characterized by single-crystal X-ray diffraction and several other techniques. The resulting complex works as a noble-metal-free catalyst for visible-light-driven catalytic generation of hydrogen, by coupling with a [Ir(coumarin)2(dtbbpy)][PF6] photosensitizer and a triethanolamine (TEOA) sacrificial electron donor. Under minimally optimized conditions, a turnover number (TON) of 842 was achieved for TBA-Ni16P4(SiW9)3-catalyzed hydrogen evolution system. The structural stability of TBA-Ni16P4(SiW9)3 catalyst under photocatalytic conditions was evaluated by the mercury-poisoning test, FT-IR, and DLS measurements. The photocatalytic mechanism was elucidated by both time-solved luminescence decay and static emission quenching measurements.
Influence of High-Pressure Homogenization on the Physicochemical Properties and Betalain Pigments of Red Beetroot (Beta vulgaris L.) Juice
Authors: Kruszewski Bartosz , Domian Ewa , Nowacka Małgorzata
Published: February 2023

High-pressure homogenization (HPH) is considered an innovative and modern method of processing and preserving liquid and semi-liquid foods. The aim of this research was to examine the impact of HPH processing on the content of betalain pigments and physicochemical properties of beetroot juice. Combinations of the following HPH parameters were tested: the pressure used (50, 100, 140 MPa), the number of cycles (1 and 3) and the applied cooling or no cooling. The physicochemical analysis of the obtained beetroot juices was based on the determination of the extract, acidity, turbidity, viscosity and color values. Use of higher pressures and a greater number of cycles reduces the turbidity (NTU) of the juice. Moreover, in order to maintain the highest possible extract content and a slight color change of the beetroot juice, it was crucial to perform sample cooling after the HPH process. The quantitative and qualitative profiles of betalains have been also determined in the juices. In terms of the content of betacyanins and betaxanthins, the highest values were found in untreated juice at 75.3 mg and 24.8 mg per 100 mL, respectively. The high-pressure homogenization process resulted in a decrease in the content of betacyanins in the range of 8.5–20.2% and of betaxanthins in the range of 6.5–15.0%, depending on the parameters used. Studies have shown that that the number of cycles was irrelevant, but an increase in pressure from 50 MPa to 100 or 140 MPa had a negative effect on pigment content. Additionally, juice cooling significantly limits the degradation of betalains in beetroot juice.
Fabrication and Evaluation of Polyvinyl Alcohol/Corn Starch/Patchouli Oil Hydrogel Films Loaded with Silver Nanoparticles Biosynthesized in Pogostemon cablin Benth Leaves’ Extract
Authors: Khairan Khairan , Hasan Miftahul , Idroes Rinaldi , Diah Muhammad
Published: February 2023

Research on the manufacture of hydrogel films from polyvinyl alcohol, corn starch, patchouli oil, and silver nanoparticles, (PVA/CS/PO/AgNPs, respectively) was completed. The silver nanoparticles used in this study resulted from green synthesis using local patchouli plants (Pogostemon cablin Benth). Aqueous patchouli leaf extract (APLE) and methanol patchouli leaf extract (MPLE) are used in the synthesis of phytochemicals (green synthesis), which are then blended in the production of PVA/CS/PO/AgNPs hydrogel films, which are then cross linked with glutaraldehyde. The results demonstrated that the hydrogel film was flexible, easy to fold, and free of holes and air bubbles. The presence of hydrogen bonds between the functional groups of PVA, CS, and PO was revealed using FTIR spectroscopy. SEM analysis revealed that the hydrogel film was slightly agglomerated and did not exhibit cracking or pinholes. The analysis of pH, spreadability, gel fraction, and swelling index showed that the resulting PVA/CS/PO/AgNP hydrogel films met expected standards except for the organoleptic properties of the resulting colors, which tended to be slightly darker in color. The formula with silver nanoparticles synthesized in methanolic of patchouli leaf extract (AgMENPs) had the highest thermal stability compared to hydrogel films with silver nanoparticles synthesized in aqueous of patchouli leaf extract (AgAENPs). The hydrogel films can be safely used up to 200 °C. The antibacterial studies revealed that the films inhibited the growth of both Staphylococcus aureus and Staphylococcus epidermis, as determined by the disc diffusion method, with the best antibacterial activity being against Staphylococcus aureus. In conclusion, the hydrogel film F1, loaded with silver nanoparticles biosynthesized in aqueous of patchouli leave extract (AgAENPs) and light fraction of patchouli oil (LFoPO) performed the best activity against both Staphylococcus aureus and Staphylococcus epidermis.
Synthesis and Characterization of Novel Triphenylamine—Containing Electrochromic Polyimides with Benzimidazole Substituents
Authors: Yan Kuangguo , Chen Haiquan , Zhu Chenjie , Ke Zhao , Li Dongwu , Wang Mengxia , Dai Fengna , Yu Youhai
Published: February 2023

Two novel electrochromic aromatic polyimides (named as TPA-BIA-PI and TPA-BIB-PI, respectively) with pendent benzimidazole group were synthesized from 1,2-Diphenyl-N,N′-di-4-aminophenyl-5-amino-benzimidazole and 4-Amino-4′-aminophenyl-4″-1-phenyl-benzimidazolyl-phenyl-aniline with 4,4′-(hexafluoroisopropane) phthalic anhydride (6FDA) via two-step polymerization process, respectively. Then, polyimide films were prepared on ITO-conductive glass by electrostatic spraying, and their electrochromic properties were studied. The results showed that due to the π-π* transitions, the maximum UV–Vis absorption bands of TPA-BIA-PI and TPA-BIB-PI films were located at about 314 nm and 346 nm, respectively. A pair of reversible redox peaks of TPA-BIA-PI and TPA-BIB-PI films that were associated with noticeable color changed from original yellow to dark blue and green were observed in the cyclic voltammetry (CV) test. With increasing voltage, new absorption peaks of TPA-BIA-PI and TPA-BIB-PI films emerged at 755 nm and 762 nm, respectively. The switching/bleaching times of TPA-BIA-PI and TPA-BIB-PI films were 13 s/16 s and 13.9 s/9.5 s, respectively, showing that these polyimides can be used as novel electrochromic materials.
Enhancing the Antimicrobial Effect of Ozone with Mentha piperita Essential Oil
Authors: Floare Alin-Daniel , Dumitrescu Ramona , Alexa Vlad Tiberiu , Balean Octavia , Szuhanek Camelia , Obistioiu Diana , Cocan Ileana , Neacsu Alina-Georgeta , Popescu Iuliana , Fratila Aurora Doris , Galuscan Atena
Published: February 2023

This study aimed to obtain and analyse Mentha piperita essential oil (MpEO) for the prospect of being used as an enhancement agent for the antimicrobial potential of ozone against gram-positive and gram-negative bacteria and fungi. The research was done for different exposure times, and it gained time–dose relationships and time–effect correlations. Mentha piperita (Mp) essential oil (MpEO) was obtained via hydrodistillation and further analysed by using GC-MS. The broth microdilution assay was used to determine the strain inhibition/strain mass growth by using spectrophotometric optical density reading (OD). The bacterial/mycelium growth rates (BGR/MGR) and the bacterial/mycelium inhibition rates (BIR/MIR) after ozone treatment in the presence and absence of MpEO on the ATTC strains were calculated; the minimum inhibition concentration (MIC) and statistical interpretations of the time–dose relationship and specific t-test correlations were determined. The effect of ozone on the following tested strains at maximum efficiency was observed after 55 s of single ozone exposure, in order of effect strength: S. aureus > P. aeruginosa > E. coli > C. albicans > S. mutans. For ozone with the addition of 2% MpEO (MIC), maximum efficacy was recorded at 5 s for these strains, in order of effect strength: C. albicans > E. coli > P. aeruginosa > S. aureus > S. mutans. The results suggest a new development and affinity regarding the cell membrane of the different microorganisms tested. In conclusion, the use of ozone, combined with MpEO, is sustained as an alternative therapy in plaque biofilm and suggested as helpful in controlling oral disease-causing microorganisms in medicine.
A Novel Approach in Skin Care: By-Product Extracts as Natural UV Filters and an Alternative to Synthetic Ones
Authors: Ferreira Sara M. , Gomes Sandra M. , Santos Lúcia
Published: February 2023

The cosmetic industry has been focusing on replacing synthetic ingredients with natural ones, taking advantage of their bioactive compounds. This work assessed the biological properties of onion peel (OP) and passion fruit peel (PFP) extracts in topical formulations as an alternative to synthetic antioxidants and UV filters. The extracts were characterized regarding their antioxidant capacity, antibacterial capacity and sun protection factor (SPF) value. Results revealed that the OP extract exhibited better results, which can result from the high concentrations of quercetin, as identified and quantified in HPLC analysis. Afterward, nine formulations of O/W creams were produced with minor changes in the quantity of additives: OP and PFP extract (natural antioxidants and UV filters), BHT (synthetic antioxidant) and oxybenzone (synthetic UV filter). The stability of the formulations was determined for 28 days; it was verified that they remained stable throughout the study period. The assays of the formulations’ antioxidant capacity and SPF value revealed that OP and PFP extracts have some photoprotective properties and are excellent sources of antioxidants. As a result, they can be incorporated in daily moisturizers with SPF and sunscreens replacing and/or diminishing the quantities of synthetic ingredients, reducing their negative effects on human health and the environment.