bluuebunny/crossref_metadata_2025 huggingface dataset:
Hepatoprotective and Renoprotective Properties of Lovastatin-Loaded Ginger and Garlic Oil Nanoemulsomes: Insights into Serum Biological Parameters
Authors: Faran Syed Ali , Asghar Sajid , Khalid Syed Haroon , Khan Ikram Ullah , Asif Muhammad , Khalid Ikrima , Farooq Gohar Umar , Hussain Tanveer
Published: September 2019

Background and Objectives: Dyslipidemia is gaining much attention among healthcare professionals because of its high association with the malfunctioning of a number of normal physiological and metabolic processes in the body. Obesity is directly interconnected with dyslipidemia and is said to be a denouement of hyperlipidemia and, if left untreated, may lead to intense damage to organs that are directly involved in fat metabolism. The objective of this study was to investigate the synergistic antiobesity and anti-hyperlipidemic activities along with hepato- and renoprotective potential of nanoemulsomes (NES) of lovastatin (LTN)-loaded ginger (GR) and garlic (GL) oils. Materials and Methods: LTN nanoemulsomes co-encapsulated with GR oil and GL oil were prepared by a thin hydration technique. Eight-week-old male Wistar rats weighing 200–250 g were induced with hyperlipidemia via a high-fat diet (HFD) comprising 40% beef tallow. Body weight, serum biochemical lipid parameters, and those for liver and kidney functions, serum TC, LDL-C, vLDL-C, HDL-C, TG, atherogenic index (AI), ALT, AFT, ALP, γ-GT, total protein (TP), serum albumin and globulin ratio (A/G), serum creatinine, blood urea nitrogen (BUN) and blood urea, and histopathology of hematoxylin and eosin (H&E) stained liver and kidney sections of all aforementioned groups were examined in the treated animals. Results: Nanoemulsomes of LTN-loaded GR and GL oils provided synergistic effects with LTN, exerted better ameliorative actions in reducing serum TC, LDL-C, vLDL-C, triglycerides, and AI, and improved serum HDL-C levels. Serum ALT, AST, ALP, and γ-GT levels were in the normal range for nanoemulsome groups. H&E stained liver and kidney sections of these animals confirmed better hepatoprotective and renoprotective effects than LTN alone. Serum biochemical parameters for renal functions also claimed to be in the moderate range for nanoemulsome-treated groups. Conclusion: This study demonstrated that nanoemulsomes of LTN-loaded GR and GL oils synergistically provided better antihyperlipidemic, hepatoprotective, and renoprotective effects as compared to LTN alone.
Retention-Aware DRAM Auto-Refresh Scheme for Energy and Performance Efficiency
Authors: Cheng Wei-Kai , Shen Po-Yuan , Li Xin-Lun
Published: September 2019

Dynamic random access memory (DRAM) circuits require periodic refresh operations to prevent data loss. As DRAM density increases, DRAM refresh overhead is even worse due to the increase of the refresh cycle time. However, because of few the cells in memory that have lower retention time, DRAM has to raise the refresh frequency to keep the data integrity, and hence produce unnecessary refreshes for the other normal cells, which results in a large refresh energy and performance delay of memory access. In this paper, we propose an integration scheme for DRAM refresh based on the retention-aware auto-refresh (RAAR) method and 2x granularity auto-refresh simultaneously. We also explain the corresponding modification need on memory controllers to support the proposed integration refresh scheme. With the given profile of weak cells distribution in memory banks, our integration scheme can choose the most appropriate refresh technique in each refresh time. Experimental results on different refresh cycle times show that the retention-aware refresh scheme can properly improve the system performance and have a great reduction in refresh energy. Especially when the number of weak cells increased due to the thermal effect of 3D-stacked architecture, our methodology still keeps the same performance and energy efficiency.
Staphylococcus xylosus Infection in Rainbow Trout (Oncorhynchus mykiss) As a Primary Pathogenic Cause of Eye Protrusion and Mortality
Authors: Oh Woo Taek , Jun Jin Woo , Giri Sib Sankar , Yun Saekil , Kim Hyoun Joong , Kim Sang Guen , Kim Sang Wha , Han Se Jin , Kwon Jun , Park Se Chang
Published: September 2019

Staphylococcal infections are extensively investigated in humans owing to the resistance of staphylococci to diverse antibiotics commonly used in hospitals. The resistance mechanism of methicillin-resistant Staphylococcus aureus has garnered the interest of researchers due to its risk to the global public health. Furthermore, the zoonotic potential of staphylococci has led to increased interest in their transmission mechanism via food, livestock, as well as domestic and wild animals. Although fish are globally consumed, there are only few studies on the potential threat of staphylococcal infection in aquatic animals. In this study, we present the first description of Staphylococcus xylosus infection and its pathogenicity in rainbow trout, which resulted in fish mortality and economic losses in trout fisheries. We focused on the pathogenic role of the bacterium and its influence on rainbow trout based on the clinical symptoms in the eyes. Staphylococcus xylosus infection induced exophthalmia and disrupted the primary immune barrier, which increased the possibility of other secondary bacterial infections in fish under poor conditions, resulting in continuous mortality.
Microbial Assemblages in Pressurized Antarctic Brine Pockets (Tarn Flat, Northern Victoria Land): A Hotspot of Biodiversity and Activity
Authors: Papale Maria , Lo Giudice Angelina , Conte Antonella , Rizzo Carmen , Rappazzo Alessandro C. , Maimone Giovanna , Caruso Gabriella , La Ferla Rosabruna , Azzaro Maurizio , Gugliandolo Concetta , Paranhos Rodolfo , Cabral Anderson S. , Romano Spica Vincenzo , Guglielmin Mauro
Published: September 2019

Two distinct pressurized hypersaline brine pockets (named TF4 and TF5), separated by a thin ice layer, were detected below an ice-sealed Antarctic lake. Prokaryotic (bacterial and archaeal) diversity, abundances (including virus-like particles) and metabolic profiles were investigated by an integrated approach, including traditional and new-generation methods. Although similar diversity indices were computed for both Bacteria and Archaea, distinct bacterial and archaeal assemblages were observed. Bacteroidetes and Gammaproteobacteria were more abundant in the shallowest brine pocket, TF4, and Deltaproteobacteria, mainly represented by versatile sulphate-reducing bacteria, dominated in the deepest, TF5. The detection of sulphate-reducing bacteria and methanogenic Archaea likely reflects the presence of a distinct synthrophic consortium in TF5. Surprisingly, members assigned to hyperthermophilic Crenarchaeota and Euryarchaeota were common to both brines, indicating that these cold habitats host the most thermally tolerant Archaea. The patterns of microbial communities were different, coherently with the observed microbiological diversity between TF4 and TF5 brines. Both the influence exerted by upward movement of saline brines from a sub-surface anoxic system and the possible occurrence of an ancient ice remnant from the Ross Ice Shelf were the likely main factors shaping the microbial communities.
A Glimpse into Genetic Diversity and Symbiont Interaction Patterns in Lichen Communities from Areas with Different Disturbance Histories in Białowieża Forest, Poland
Authors: Singh Garima , Kukwa Martin , Dal Grande Francesco , Łubek Anna , Otte Jürgen , Schmitt Imke
Published: September 2019

Anthropogenic disturbances can have strong impacts on lichen communities, as well as on individual species of lichenized fungi. Traditionally, lichen monitoring studies are based on the presence and abundance of fungal morphospecies. However, the photobionts, as well photobiont mycobiont interactions also contribute to the structure, composition, and resilience of lichen communities. Here we assess the genetic diversity and interaction patterns of algal and fungal partners in lichen communities along an anthropogenic disturbance gradient in Białowieża Forest (Poland). We sampled a total of 224 lichen thalli in a protected, a managed, and a disturbed area of the forest, and sequenced internal transcribed spacer (ITS) ribosomal DNA (rDNA) of both, fungal and algal partners. Sequence clustering using a 97% similarity threshold resulted in 46 fungal and 23 green algal operational taxonomic units (OTUs). Most of the recovered photobiont OTUs (14 out of 23) had no similar hit in the NCBI-BLAST search, suggesting that even in well studied regions, such as central Europe, a lot of photobiont diversity is yet undiscovered. If a mycobiont was present at more than one site, it was typically associated with the same photobiont OTU(s). Generalist species, i.e., taxa that associate with multiple symbiont partners, occurred in all three disturbance regimes, suggesting that such taxa have few limitations in colonizing or persisting in disturbed areas. Trebouxia jamesii associated with 53% of the fungal OTUs, and was generally the most common photobiont OTU in all areas, implying that lichens that associate with this symbiont are not limited by the availability of compatible photobionts in Central European forests, regardless of land use intensity.
Determination of the Total Phenolic Content in Wine Samples Using Potentiometric Method Based on Permanganate Ion as an Indicator
Authors: Sun Ziqi , Zhang Yufeng , Xu Xinyue , Wang Minglin , Kou Lijuan
Published: September 2019

A rapid and accurate determination method for total phenolic content is of great importance for controlling the quality of wine samples. A promising potentiometric detection approach, based on permanganate ion fluxes across ion-selective electrode membranes, is fabricated for measuring the total phenolic content of wine. The results show that the presence of phenols, such as gallic acid, leads to a potential increase for the potentiometric sensor. Additionally, the present sensor exhibits a linear potential response with the concentration range from 0.05 to 3.0 g/L with a detection limit of 6.6 mg/L calculated using gallic acid. These sensors also exhibit a fast response time, an acceptable reproducibility and long-term stability. These results indicate that the proposed potentiometric sensor can be a promising and reliable tool for the rapid determination of total phenolic content in wine samples.
Effect of Short-Term Increase in Meal Frequency on Glucose Metabolism in Individuals with Normal Glucose Tolerance or Impaired Fasting Glucose: A Randomized Crossover Clinical Trial
Authors: Hibi Masanobu , Hari Sayaka , Yamaguchi Tohru , Mitsui Yuki , Kondo Sumio , Katashima Mitsuhiro
Published: September 2019

Effects of meal frequency on blood glucose levels and glucose metabolism were evaluated over 3 days in adult males with normal glucose tolerance (NGT, n = 9) or impaired fasting glucose (IFG, n = 9) in a randomized, crossover comparison study. Subjects were provided with an isocaloric diet 3 times daily (3M) or 9 times daily (9M). Blood glucose was monitored on Day 3 using a continuous glucose monitoring system, and subjects underwent a 75-g oral glucose tolerance test (OGTT) on Day 4. Daytime maximum blood glucose, glucose range, duration of glucose ≥180 mg/dL, and nighttime maximum glucose were significantly lower in the NGT/9M condition than in the NGT/3M condition. Similar findings were observed in the IFG subjects, with a lower daytime and nighttime maximum glucose and glucose range, and a significantly higher daytime minimum glucose in the 9M condition than in the 3M condition. The OGTT results did not differ significantly between NGT/3M and NGT/9M conditions. In contrast, the incremental area under the curve tended to be lower and the maximum plasma glucose concentration was significantly lower in the IFG/9M condition than in the IFG/3M condition. In IFG subjects, the 9M condition significantly improved glucose metabolism compared with the 3M condition. Higher meal frequency may increase glucagon-like peptide 1 secretion and improve insulin secretion.
New and Preliminary Evidence on Altered Oral and Gut Microbiota in Individuals with Autism Spectrum Disorder (ASD): Implications for ASD Diagnosis and Subtyping Based on Microbial Biomarkers
Authors: Kong Xuejun , Liu Jun , Cetinbas Murat , Sadreyev Ruslan , Koh Madelyn , Huang Hui , Adeseye Adetaye , He Puhan , Zhu Junli , Russell Hugh , Hobbie Clara , Liu Kevi , Onderdonk Andrew B.
Published: September 2019

Autism Spectrum Disorder (ASD) is a complex neurological and developmental disorder characterized by behavioral and social impairments as well as multiple co-occurring conditions, such as gastrointestinal abnormalities, dental/periodontal diseases, and allergies. The etiology of ASD likely involves interaction between genetic and environmental factors. Recent studies suggest that oral and gut microbiome play important roles in the pathogenesis of inflammation, immune dysfunction, and disruption of the gut–brain axis, which may contribute to ASD pathophysiology. The majority of previous studies used unrelated neurotypical individuals as controls, and they focused on the gut microbiome, with little attention paid to the oral flora. In this pilot study, we used a first degree-relative matched design combined with high fidelity 16S rRNA (ribosomal RNA) gene amplicon sequencing in order to characterize the oral and gut microbiotas of patients with ASD compared to neurotypical individuals, and explored the utility of microbiome markers for ASD diagnosis and subtyping of clinical comorbid conditions. Additionally, we aimed to develop microbiome biomarkers to monitor responses to a subsequent clinical trial using probiotics supplementation. We identified distinct features of gut and salivary microbiota that differed between ASD patients and neurotypical controls. We next explored the utility of some differentially enriched markers for ASD diagnosis and examined the association between the oral and gut microbiomes using network analysis. Due to the tremendous clinical heterogeneity of the ASD population, we explored the relationship between microbiome and clinical indices as an attempt to extract microbiome signatures assocociated with clinical subtypes, including allergies, abdominal pain, and abnormal dietary habits. The diagnosis of ASD currently relies on psychological testing with potentially high subjectivity. Given the emerging role that the oral and gut microbiome plays in systemic diseases, our study will provide preliminary evidence for developing microbial markers that can be used to diagnose or guide treatment of ASD and comorbid conditions. These preliminary results also serve as a starting point to test whether altering the oral and gut microbiome could improve co-morbid conditions in patients with ASD and further modify the core symptoms of ASD.
Trans Fat Labeling Information on Brazilian Packaged Foods
Authors: Ricardo Camila Zancheta , Peroseni Isabela Mateus , Mais Laís Amaral , Martins Ana Paula Bortoletto , Duran Ana Clara
Published: September 2019

Although the adverse effects of trans fat consumption are well documented, industrially-produced trans fats are still used in a variety of food products. Our objective was to investigate the presence of trans fat information on the nutrition facts panel, in the list of ingredients, and the use of trans fat claims in packaged food and beverages marketed in Brazil. This was a cross-sectional study that used data from packaged food and beverages available in the five supermarket chains with the largest market share in Brazil. Of the 11,434 products that were analyzed, 81.3% did not present a source of trans fats in the list of ingredients. The percentages of products with specific (hydrogenated fats or oils) and unspecific trans fat terms (margarine, vegetable fat, and vegetable cream) in the list of ingredients were 4.1% and 14.6%, respectively. Bakery products, cookies and crackers, candies and desserts, snacks, and convenience foods had the highest percentages of trans fat claims. We also found claims in products with ingredients that are sources of trans fats. In conclusion, trans fat ingredients were found in almost one-fifth of the Brazilian packaged foods. The current Brazilian legislation is not sufficient to inform consumers about the content of trans fats in packaged foods. Along with measures to restrict the use of industrially-produced trans fats, improvements in nutritional labeling are also needed.
Micronutrient Adequacy in Preschool Children Attending Family Child Care Homes
Authors: Cuadrado-Soto Esther , Risica Patricia Markham , Gans Kim M. , Mena Noereem Z. , Ellis Carolyn , Araujo Carolina D. , Lofgren Ingrid E. , Stowers Kristen Cooksey , Tovar Alison
Published: September 2019

Limited data is available on the micronutrient intake and adequacy in preschool children enrolled in family child care homes (FCCH). The goal of this paper is to describe the micronutrient adequacy relative to age-specific recommendations of preschool-aged children (aged 2–5 years) attending FCCH in Rhode Island (RI). Dietary data among younger preschoolers (aged 2–3 years), n = 245) and older preschoolers (aged 4–5 years), n = 121) in 118 RI FCCH (N = 366 children) were analyzed. Nutrient adequacy was assessed as the amount of nutrient per 1000 kcal of the diet that would meet the Institute of Medicine nutrient requirements (critical nutrient density), and it was compared to the observed nutrient densities of the children. The sodium:potassium ratio was also calculated. For most micronutrients, the observed density met or exceeded the recommendation, meaning the children’s intake was adequate. However, a high proportion of children had nutrient densities under the recommendation for vitamins D, E, K, and potassium (86.1%, 89.1%, 70.8%, and 99.2% of children, respectively). The mean vitamin B12, potassium, and zinc densities were statistically higher in younger vs. older preschoolers (p < 0.05 for all). Low densities in calcium and vitamins K and B5 were more frequent in older children vs. younger children (p < 0.05). In addition, older preschoolers had a higher sodium:potassium ratio than younger children (p < 0.05). The micronutrient intake density was adequate for most nutrients. However, intake of some nutrients was of concern. Further attention to training and compliance in FCCH may improve the diet quality of those cared for in these settings.
Bisphenol A Analogues in Food and Their Hormonal and Obesogenic Effects: A Review
Authors: Andújar Natalia , Gálvez-Ontiveros Yolanda , Zafra-Gómez Alberto , Rodrigo Lourdes , Álvarez-Cubero María Jesús , Aguilera Margarita , Monteagudo Celia , Rivas Ana
Published: September 2019

Bisphenol A (BPA) is the most well-known compound from the bisphenol family. As BPA has recently come under pressure, it is being replaced by compounds very similar in structure, but data on the occurrence of these BPA analogues in food and human matrices are limited. The main objective of this work was to investigate human exposure to BPA and analogues and the associated health effects. We performed a literature review of the available research made in humans, in in vivo and in vitro tests. The findings support the idea that exposure to BPA analogues may have an impact on human health, especially in terms of obesity and other adverse health effects in children.
Vitamin D Modulates the Response of Bronchial Epithelial Cells Exposed to Cigarette Smoke Extract
Authors: Mathyssen Carolien , Serré Jef , Sacreas Annelore , Everaerts Stephanie , Maes Karen , Verleden Stijn , Verlinden Lieve , Verstuyf Annemieke , Pilette Charles , Gayan-Ramirez Ghislaine , Vanaudenaerde Bart , Janssens Wim
Published: September 2019

In chronic obstructive pulmonary disease (COPD), the bronchial epithelium is the first immune barrier that is triggered by cigarette smoke. Although vitamin D (vitD) has proven anti-inflammatory and antimicrobial effects in alveolar macrophages, little is known about the direct role of vitD on cigarette smoke-exposed bronchial epithelial cells. We examined the effects of vitD on a human bronchial epithelial cell line (16HBE) and on air–liquid culture of primary bronchial epithelial cells (PBEC) of COPD patients and controls exposed for 24 h to cigarette smoke extract (CSE). VitD decreased CSE-induced IL-8 secretion by 16HBE cells, but not by PBEC. VitD significantly increased the expression of the antimicrobial peptide cathelicidin in 16HBE and PBEC of both COPD subjects and controls. VitD did not affect epithelial to mesenchymal transition or epithelial MMP-9 expression and was not able to restore impaired wound healing by CSE in 16HBE cells. VitD increased the expression of its own catabolic enzyme CYP24A1 thereby maintaining its negative feedback. In conclusion, vitD supplementation may potentially reduce infectious exacerbations in COPD by the upregulation of cathelicidin in the bronchial epithelium.
Distinct TP53 Mutation Subtypes Differentially Influence Cellular Iron Metabolism
Authors: Clarke Stephen L. , Thompson Laurie R. , Dandekar Eshan , Srinivasan Aishwarya , Montgomery McKale R.
Published: September 2019

The most commonly mutated gene in all human cancers is the tumor suppressor gene TP53; however, in addition to the loss of tumor suppressor functions, mutations in TP53 can also promote cancer progression by altering cellular iron acquisition and metabolism. The primary objective of this work was to determine how TP53 mutation status influences the molecular control of iron homeostasis. The effect of TP53 mutation type on cellular iron homeostasis was examined using cell lines with inducible versions of either wild-type TP53 or a representative mutated TP53 gene from exemplary “hotspot” mutations in the DNA binding domain (R248, R273, and R175) as well as H193Y. The introduction of distinct TP53 mutation types alone was sufficient to disrupt cellular iron metabolism. These effects were mediated, at least in part, due to differences in the responsiveness of iron regulatory proteins (IRPs) to cellular iron availability. IRPs are considered the master regulators of intracellular iron homeostasis because they coordinate the expression of iron storage (ferritin) and iron uptake (transferrin receptor) genes. In response to changes in iron availability, cells harboring either a wild-type TP53 or R273H TP53 mutation displayed canonical IRP-mediated responses, but neither IRP1 RNA binding activity nor IRP2 protein levels were affected by changes in iron status in cells harboring the R175H mutation type. However, all mutation types exhibited robust changes in ferritin and transferrin receptor protein expression in response to iron loading and iron chelation, respectively. These findings suggest a novel, IRP-independent mode of iron regulation in cells expressing distinct TP53 mutations. As TP53 is mutated in nearly half of all human cancers, and iron is necessary for cancer cell growth and proliferation, the studies have implications for a wide range of clinically important cancers.
In Vitro Antithrombotic and Hypocholesterolemic Activities of Milk Fermented with Specific Strains of Lactococcus lactis
Authors: Rendon-Rosales Miguel Ángel , Torres-Llanez María J. , González-Córdova Aarón F. , Hernández-Mendoza Adrián , Mazorra-Manzano Miguel A. , Vallejo-Cordoba Belinda
Published: September 2019

Milk fermented with specific lactic acid bacteria (LAB) was reported to be a rich source of metabolites, such as peptides with different biological activities that may have a positive effect on cardiovascular health. Thus, in this study, the antithrombotic and hypocholesterolemic activities of fermented milk with specific strains of Lactococcus lactis were investigated before and after exposure to a simulated gastrointestinal digestion (SGD) model. The inhibition of thrombin-induced fibrin polymerization (IC50 peptide concentration necessary to inhibit thrombin activity by 50%), anticoagulant activity, inhibition of micellar solubility of cholesterol and bile acid binding capacity of water soluble fractions (WSF) <3 kDa from fermented milk were evaluated. Results indicated that the WSF from fermented milk with Lc-572 showed antithrombotic (IC50 = 0.049 mg/mL) and hypocholesterolemic (55% inhibition of micellar solubility of cholesterol and 27% bile acid binding capacity) activities. Meanwhile, fermented milk with Lc-571 showed mainly antithrombotic activity (IC50 = 0.045 mg/mL). On the other hand, fermented milk with Lc-600 presented mainly hypocholesterolemic activity (31.4% inhibition of micellar solubility of and 70% bile acid binding capacity). Moreover, biological activities were not lost after simulated gastrointestinal digestion conditions. Thus, fermented milk with these specific L. lactis strains show potential for the development of functional foods.
Circulating Vitamin E Levels and Risk of Coronary Artery Disease and Myocardial Infarction: A Mendelian Randomization Study
Authors: Wang Tao , Xu Lin
Published: September 2019

Observational studies have reported a cardioprotective effect of vitamin E whereas intervention trials failed to confirm its beneficial effects, and even some reported adverse effects of vitamin E supplements on coronary artery disease (CAD). To clarify, we conducted a two-sample mendelian randomization study to investigate causal association of vitamin E with the risk of CAD. Three single nucleotide polymorphisms (SNPs) identified in a genome-wide analysis study including 7781 individuals of European descent, rs964184, rs2108622, and rs11057830 were used as the genetic instruments for vitamin E. Data for CAD/myocardial infarction (MI) were available from Coronary ARtery DIsease Genome wide Replication and Meta-analysis (CARDIoGRAM) plus The Coronary Artery Disease (C4D) Genetics consortium. The effect of each SNP on CAD/myocardial infarction (MI) was weighted by its effect on serum vitamin E (mg/L), and results were pooled to give a summary estimates for the effect of increased vitamin E on risk of CAD/MI. Based on 3 SNPs each 1 mg/L increase in vitamin E was significantly associated with CAD (odds ratio (OR) 1.05, 95% confidence interval (CI) 1.03–1.06), MI (OR 1.04, 95% CI 1.03–1.05), elevated low-density lipoprotein cholesterol (0.021 standard deviations (SD), 95% CI 0.016, 0.027), triglycerides (0.026 SD, 95% CI 0.021, 0.031), and total cholesterol (0.043 SD, 95% CI 0.038, 0.048) and lower levels of high-density lipoprotein cholesterol (−0.019 SD 95% CI −0.024, −0.014). Our findings indicate that higher vitamin E may increase the risk of CAD/MI and the safety and efficacy of vitamin E supplementation use should be reevaluated.
Relationship between Changes in Microbiota and Liver Steatosis Induced by High-Fat Feeding—A Review of Rodent Models
Authors: Gómez-Zorita Saioa , Aguirre Leixuri , Milton-Laskibar Iñaki , Fernández-Quintela Alfredo , Trepiana Jenifer , Kajarabille Naroa , Mosqueda-Solís Andrea , González Marcela , Portillo María P.
Published: September 2019

Several studies have observed that gut microbiota can play a critical role in nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) development. The gut microbiota is influenced by different environmental factors, which include diet. The aim of the present review is to summarize the information provided in the literature concerning the impact of changes in gut microbiota on the effects which dietary fat has on liver steatosis in rodent models. Most studies in which high-fat feeding has induced steatosis have reported reduced microbiota diversity, regardless of the percentage of energy provided by fat. At the phylum level, an increase in Firmicutes and a reduction in Bacteroidetes is commonly found, although widely diverging results have been described at class, order, family, and genus levels, likely due to differences in experimental design. Unfortunately, this fact makes it difficult to reach clear conclusions concerning the specific microbiota patterns associated with this feeding pattern. With regard to the relationship between high-fat feeding-induced changes in liver and microbiota composition, although several mechanisms such as alteration of gut integrity and increased permeability, inflammation, and metabolite production have been proposed, more scientific evidence is needed to address this issue and thus further studies are needed.
Cost-Minimized Nutritionally Adequate Food Baskets as Basis for Culturally Adapted Dietary Guidelines for Ethiopians
Authors: Gurmu Abdi Bekele , Nykänen Esa-Pekka A. , Alemayehu Fikadu Reta , Robertson Aileen , Parlesak Alexandr
Published: September 2019

The high prevalence of undernutrition, especially stunting, in Ethiopia hampers the country’s economic productivity and national development. One of the obstacles to overcome undernutrition is the relatively high cost of food for low economic groups. In this study, linear programming was used to (i) identify urban and rural nutritionally adequate food baskets (FBs) with the highest affordability for an Ethiopian family of five and (ii) create urban and rural FBs, optimized for cultural acceptability, which are affordable for a family with the lowest income. Nutritionally adequate rural and urban FBs with highest affordability cost as little as Ethiopian Birr (ETB) 31 and 38 (~USD 1.07 and 1.31), respectively, but have poor dietary diversity (16 and 19 foods). FBs that cost ETB 71.2 (~USD 2.45) contained 64 and 48 foods, respectively, and were much more similar to the food supply pattern reported by FAO (15% and 19% average relative deviation per food category). The composed FBs, which are affordable for the greater part of the Ethiopian population, may serve as a basis for the development of culturally acceptable food-based dietary guidelines. These guidelines would recommend a diet composed of approximately up to 60% cereals, up to 20% roots and tubers, 10% legumes, and 10% fruits and vegetables by weight, plus only a small share from animal foods.
Effects of Exercise and Nutritional Intervention on Body Composition, Metabolic Health, and Physical Performance in Adults with Sarcopenic Obesity: A Meta-Analysis
Authors: Hsu Kuo-Jen , Liao Chun-De , Tsai Mei-Wun , Chen Chiao-Nan
Published: September 2019

People with sarcopenic obesity (SO) are characterized by both low muscle mass (sarcopenia) and high body fat (obesity); they have greater risks of metabolic diseases and physical disability than people with sarcopenia or obesity alone. Exercise and nutrition have been reported to be effective for both obesity and sarcopenia management. Thus, we aimed to investigate the effects of exercise and nutrition on body composition, metabolic health, and physical performance in individuals with SO. Studies investigating the effects of exercise and nutrition on body composition, metabolic health, and physical performance in SO individuals were searched from electronic databases up to April 2019. Fifteen studies were included in the meta-analysis. Aerobic exercise decreased body weight and fat mass (FM). Resistance exercise (RE) decreased FM and improved grip strength. The combination of aerobic exercise and RE decreased FM and improved walking speed. Nutritional intervention, especially low-calorie high-protein (LCHP) diet, decreased FM but did not affect muscle mass and grip strength. In addition to exercise training, nutrition did not provide extra benefits in outcome. Exercise, especially RE, is essential to improve body composition and physical performance in individuals with SO. Nutritional intervention with LCHP decreases FM but does not improve physical performance.
Recycling of Polypropylene/Polyethylene Blends: Effect of Chain Structure on the Crystallization Behaviors
Authors: Aumnate Chuanchom , Rudolph Natalie , Sarmadi Majid
Published: September 2019

The combination of high-density polyethylene (HDPE), low-density polyethylene (LDPE) and polypropylene (PP) is frequently found in polymer waste streams. Because of their similar density, they cannot be easily separated from each other in the recycling stream. Blending of PP/ polyethylenes (PEs) in different ratios possibly eliminate the sorting process used in the regular recycling process. PP has fascinating properties such as excellent processability and chemical resistance. However, insufficient flexibility limits its use for specific applications. Blending of PP with relative flexible PEs might improve its flexibility. This is a unique approach for recycling or upcycling, which aims to maintain or improve the properties of recycled materials. The effects of the branched-chain structures of PEs on the crystallization behavior and the related mechanical properties of such blends were investigated. The overall kinetics of crystallization of PP was significantly influenced by the presence of PEs with different branched-chain structures. The presence of LDPE was found to decrease the overall crystallization rate while the addition of HDPE accelerated the crystallization process of the blends. No negative effect on the mechanical performance and the related crystallinity was observed within the studied parameter range.
Tunable Fluorescence-Responsive Double Hydrophilic Block Polymers Induced by the Formation of Pseudopolyrotaxanes with Cucurbit[7]Uril
Authors: Qiu Xiumin , Wang Xin , Hou Shengzhen , Zhang Jin , Zhou Jing , Tan Yebang
Published: September 2019

There is an urgent need for new strategies that allow the simultaneous detection and control of drug delivery. By making use of supramolecular host-guest interactions, a kind of pseudopolyrotaxanes, as a visualizable nanoscale drug carrier has been constructed by self-assembly of cucurbit[7]uril (CB[7]) with methoxy poly(ethylene glycol)-block-quaternized poly(4-vinyl pyridine) (mPEG-b-QP4VP) using 4-(chloromethyl)benzonitrile. Simple addition of CB[7] into an aqueous solution of mPEG-b-QP4VP resulted in noncovalent attachment of CB[7] to 4-cyanobenzyl-containing polymers, transforming the nonemissive mPEG-b-QP4VP micelles into highly fluorescent micelles. These pseudopolyrotaxanes micelles exhibited remarkable supramolecular assembly-induced emission enhancement and excellent biocompatibility, showing great potential for bioimaging applications. In addition, the efficient cellular uptake of the developed pseudopolyrotaxanes micelles loaded with the anticancer drug doxorubicin was a promising platform for simultaneous cell imaging and drug delivery, thereby widening their application in cancer theranostics.
In Search of a Green Process: Polymeric Films with Ordered Arrays via a Water Droplet Technique
Authors: Yeh Shih-Chieh , Wu Chien-Hsin , Huang Ying-Chih , Lee Jen-Yu , Jeng Ru-Jong
Published: September 2019

As an efficient technique for the preparation of polymeric hexagonal orderly arrays, the breath figure (BF) process has opened a modern avenue for a bottom-up fabrication method for more than two decades. Through the use of the water vapor condensation on the solution surface, the water droplets will hexagonally pack into ordered arrays, acting as a template for controlling the regular micro patterns of polymeric films. Comparing to the top-down techniques, such as lithography or chemical etching, the use of water vapor as the template provides a simple fabrication process with sustainability. However, using highly hazardous solvents such as chloroform, carbon disulfide (CS2), benzene, dichloromethane, etc., to dissolve polymers might hinder the development toward green processes based on this technique. In this review, we will touch upon the contemporary techniques of the BF process, including its up-to-date applications first. More importantly, the search of greener processes along with less hazardous solvents for the possibility of a more sustainable BF process is the focal point of this review.
Performance and Kinetic Model of a Single-Stage Anaerobic Digestion System Operated at Different Successive Operating Stages for the Treatment of Food Waste
Authors: Pramanik Sagor Kumar , Suja Fatihah Binti , Porhemmat Mojtaba , Pramanik Biplob Kumar
Published: September 2019

A large quantity of food waste (FW) is generated annually across the world and results in environmental pollution and degradation. This study investigated the performance of a 160 L anaerobic biofilm single-stage reactor in treating FW. The reactor was operated at different hydraulic retention times (HRTs) of 124, 62, and 35 days under mesophilic conditions. The maximum biogas and methane yield achieved was 0.934 L/g VSadded and 0.607 L CH4/g VSadded, respectively, at an HRT of 124 days. When HRT decreased to 62 days, the volatile fatty acid (VFA) and ammonia accumulation increased rapidly whereas pH, methane yield, and biogas yield decreased continuously. The decline in biogas production was likely due to shock loading, which resulted in scum accumulation in the reactor. A negative correlation between biogas yield and volatile solid (VS) removal efficiency was also observed, owing to the floating scum carrying and urging the sludge toward the upper portion of the reactor. The highest VS (79%) and chemical oxygen demand (COD) removal efficiency (80%) were achieved at an HRT of 35 days. Three kinetic models—the first-order kinetic model, the modified Gompertz model, and the logistic function model—were used to fit the cumulative biogas production experimental data. The kinetic study showed that the modified Gompertz model had the best fit with the experimental data out of the three models. This study demonstrates that the stability and performance of the anaerobic digestion (AD) process, namely biogas production rate, methane yield, intermediate metabolism, and removal efficiency, were significantly affected by HRTs.
Catwalk Catholicism: On the Ongoing Significance of Federico Fellini’s Ecclesiastical Fashion Show
Authors: Inglis David , Thorpe Chris
Published: September 2019

In Fellini-Roma (1972), the film director Federico Fellini includes a sequence about an imaginary ecclesiastical fashion show, a display of ever more outlandish clerical clothing designs. Fellini brought together various elements that, in conventional cultural coding, do not seem to fit together: secular fashion design and catwalks, and Catholic practice and ceremonial. The sequence juxtaposes and intermingles these apparent incompatibles. Surprisingly little scholarly attention has been paid to the nature and significance of this sequence. Yet it is complex, being simultaneously satirical and empathetic, as well as camp and carnivalesque. The paper reaches back in time, reviewing the history of Catholic vestments, to show that the sequence also dramatizes the fact that sartorial fashion and Church garb have overlapped and informed each other historically. The appeal of the sequence for various types of audience has been enhanced in the internet age, and the paper considers how it has become an increasingly ubiquitous reference-point for the fashion industry, bloggers, and cultural critics, especially when the latter want to thematize controversies about male homosexuality in the Church today. Fellini’s presentation of catwalk Catholicism is both a rich object of scholarship, and a multivalent vehicle used by actors for various contemporary purposes.
Nighttime Reflectance Generation in the Visible Band of Satellites
Authors: Kim Kimoon , Kim Ji-Hye , Moon Yong-Jae , Park Eunsu , Shin Gyungin , Kim Taeyoung , Kim Yerin , Hong Sungwook
Published: September 2019

Visible (VIS) bands, such as the 0.675 μm band in geostationary satellite remote sensing, have played an important role in monitoring and analyzing weather and climate change during the past few decades with coarse spatial and high temporal resolution. Recently, many deep learning techniques have been developed and applied in a variety of applications and research fields. In this study, we developed a deep-learning-based model to generate non-existent nighttime VIS satellite images using the Conditional Generative Adversarial Nets (CGAN) technique. For our CGAN-based model training and validation, we used the daytime image data sets of reflectance in the Communication, Ocean and Meteorological Satellite / Meteorological Imager (COMS/MI) VIS (0.675 μm) band and radiance in the longwave infrared (10.8 μm) band of the COMS/MI sensor over five years (2012 to 2017). Our results show high accuracy (bias = −2.41 and root mean square error (RMSE) = 36.85 during summer, bias = −0.21 and RMSE = 33.02 during winter) and correlation (correlation coefficient (CC) = 0.88 during summer, CC = 0.89 during winter) of values between the observed images and the CGAN-generated images for the COMS VIS band. Consequently, our CGAN-based model can be effectively used in a variety of meteorological applications, such as cloud, fog, and typhoon analyses during daytime and nighttime.
Large Scale Agricultural Plastic Mulch Detecting and Monitoring with Multi-Source Remote Sensing Data: A Case Study in Xinjiang, China
Authors: Xiong Yuankang , Zhang Qingling , Chen Xi , Bao Anming , Zhang Jieyun , Wang Yujuan
Published: September 2019

Plastic mulching has been widely practiced in crop cultivation worldwide due to its potential to significantly increase crop production. However, it also has a great impact on the regional climate and ecological environment. More importantly, it often leads to unexpected soil pollution due to fine plastic residuals. Therefore, accurately and timely monitoring of the temporal and spatial distribution of plastic mulch practice in large areas is of great interest to assess its impacts. However, existing plastic-mulched farmland (PMF) detecting efforts are limited to either small areas with high-resolution images or coarse resolution images of large areas. In this study, we examined the potential of cloud computing and multi-temporal, multi-sensor satellite images for detecting PMF in large areas. We first built the plastic-mulched farmland mapping algorithm (PFMA) rules through analyzing its spectral, temporal, and auxiliary features in remote sensing imagery with the classification and regression tree (CART). We then applied the PFMA in the dry region of Xinjiang, China, where a water resource is very scarce and thus plastic mulch has been intensively used and its usage is expected to increase significantly in the near future. The experimental results demonstrated that the PFMA reached an overall accuracy of 92.2% with a producer’s accuracy of 97.6% and a user’s accuracy of 86.7%, and the F-score was 0.914 for the PMF class. We further monitored and analyzed the dynamics of plastic mulch practiced in Xinjiang by applying the PFMA to the years 2000, 2005, 2010, and 2015. The general pattern of plastic mulch usage dynamic in Xinjiang during the period from 2000 to 2015 was well captured by our multi-temporal analysis.
Spatial Dynamics of Invasive Para Grass on a Monsoonal Floodplain, Kakadu National Park, Northern Australia
Authors: Boyden James , Wurm Penelope , Joyce Karen E. , Boggs Guy
Published: September 2019

African para grass (Urochloa mutica) is an invasive weed that has become prevalent across many important freshwater wetlands of the world. In northern Australia, including the World Heritage landscape of Kakadu National Park (KNP), its dense cover can displace ecologically, genetically and culturally significant species, such as the Australian native rice (Oryza spp.). In regions under management for biodiversity conservation para grass is often beyond eradication. However, its targeted control is also necessary to manage and preserve site-specific wetland values. This requires an understanding of para grass spread-patterns and its potential impacts on valuable native vegetation. We apply a multi-scale approach to examine the spatial dynamics and impact of para grass cover across a 181 km2 floodplain of KNP. First, we measure the overall displacement of different native vegetation communities across the floodplain from 1986 to 2006. Using high spatial resolution satellite imagery in conjunction with historical aerial-photo mapping, we then measure finer-scale, inter-annual, changes between successive dry seasons from 1990 to 2010 (for a 48 km2 focus area); Para grass presence-absence maps from satellite imagery (2002 to 2010) were produced with an object-based machine-learning approach (stochastic gradient boosting). Changes, over time, in mapped para grass areas were then related to maps of depth-habitat and inter-annual fire histories. Para grass invasion and establishment patterns varied greatly in time and space. Wild rice communities were the most frequently invaded, but the establishment and persistence of para grass fluctuated greatly between years, even within previously invaded communities. However, these different patterns were also shown to vary with different depth-habitat and recent fire history. These dynamics have not been previously documented and this understanding presents opportunities for intensive para grass management in areas of high conservation value, such as those occupied by wild rice.
A PCA–OLS Model for Assessing the Impact of Surface Biophysical Parameters on Land Surface Temperature Variations
Authors: Firozjaei Mohammad Karimi , Alavipanah Seyed Kazem , Liu Hua , Sedighi Amir , Mijani Naeim , Kiavarz Majid , Weng Qihao
Published: September 2019

Analysis of land surface temperature (LST) spatiotemporal variations and characterization of the factors affecting these variations are of great importance in various environmental studies and applications. The aim of this study is to propose an integrated model for characterizing LST spatiotemporal variations and for assessing the impact of surface biophysical parameters on the LST variations. For this purpose, a case study was conducted in Babol City, Iran, during the period of 1985 to 2018. We used 122 images of Landsat 5, 7, and 8, and products of water vapor (MOD07) and daily LST (MOD11A1) from the MODIS sensor of the Terra satellite, as well as soil and air temperature and relative humidity data measured at the local meteorological station over 112 dates for the study. First, a single-channel algorithm was applied to estimate LST, while various spectral indices were computed to represent surface biophysical parameters, which included the normalized difference vegetation index (NDVI), soil-adjusted vegetation index (SAVI), normalized difference water index (NDWI), normalized difference built-up index (NDBI), albedo, brightness, greenness, and wetness from tasseled cap transformation. Next, a principal component analysis (PCA) was conducted to determine the degree of LST variation and the surface biophysical parameters in the temporal dimension at the pixel scale based on Landsat imagery. Finally, the relationship between the first component of the PCA of LST and each surface biophysical parameter was investigated by using the ordinary least squares (OLS) regression with both regional and local optimizations. The results indicated that among the surface biophysical parameters, variations of NDBI, wetness, and greenness had the highest impact on the LST variations with a correlation coefficient of 0.75, −0.70, and −0.44, and RMSE of 0.71, 1.03, and 1.06, respectively. The impact of NDBI, wetness, and greenness varied geographically, but their variations accounted for 43%, 38%, and 19% of the LST variation, respectively. Furthermore, the correlation coefficient and RMSE between the observed LST variation and modeled LST variation, based on the most influential biophysical factors (NDBI, wetness, and greenness) yielded 0.85 and 1.06 for the regional approach and 0.93 and 0.26 for the local approach, respectively. The results of this study indicated the use of an integrated PCA–OLS model was effective for modeling of various environmental parameters and their relationship with LST. In addition, the PCA–OLS with the local optimization was found to be more efficient than the one with the regional optimization.