bluuebunny/crossref_metadata_2025 huggingface dataset:
Retrospective Analysis of U.S. Federal Environmental Regulation
Authors: Morgenstern Richard
Published: November 2017

Retrospective, or ex post, analysis of U.S. federal regulation aims to rigorously document regulatory outcomes using cost, benefit, and distributional metrics. This paper presents nine new case studies involving a total of 34 comparisons of ex ante and ex post estimates from a diverse group of environmentally oriented rules. Despite the potential for selection bias and other limitations of the case study approach, the results suggest a slight tendency to overestimate both costs and benefits (or effectiveness) of regulation. This paper considers various analytic issues relevant to developing credible baselines for comparison, and offers policy lessons regarding the design of emissions trading programs along with approaches for incorporating uncertainty into both preregulatory studies and policy designs. Recommendations to facilitate and support future retrospective analyses are also presented.
Collective Action and Representation in Autocracies: Evidence from Russia’s Great Reforms
Authors: DOWER PAUL CASTAÑEDA , FINKEL EVGENY , GEHLBACH SCOTT , NAFZIGER STEVEN
Published: November 2017

We explore the relationship between capacity for collective action and representation in autocracies with data from Imperial Russia. Our primary empirical exercise relates peasant representation in new institutions of local self-government to the frequency of peasant unrest in the decade prior to reform. To correct for measurement error in the unrest data and other sources of endogeneity, we exploit idiosyncratic variation in two determinants of peasant unrest: the historical incidence of serfdom and religious polarization. We find that peasants were granted less representation in districts with more frequent unrest in preceding years—a relationship consistent with the Acemoglu-Robinson model of political transitions and inconsistent with numerous other theories of institutional change. At the same time, we observe patterns of redistribution in subsequent years that are inconsistent with the commitment mechanism central to the Acemoglu-Robinson model. Building on these results, we discuss possible directions for future theoretical work.
A KNIFE-ROLLER EFFECTIVELY SUBSTITUTES SOIL PREPARATION BY PLOUGH-AND-HARROW IN LOWLAND PRODUCTION SYSTEMS
Authors: THEISEN G. , SILVA J. C. C. , BASTIAANS L.
Published: November 2017

Cropping systems in the lowlands of temperate South America have been based on irrigated rice and beef-cattle production. Plough-and-harrow is still the most used method to prepare the soil after a season of irrigated rice, but it causes high soil disturbance and is time- and energy-demanding. To improve the sustainability of a rice–soybean rotation system, we studied an alternative method for soil preparation based on a heavy knife-roller. This method was evaluated during three cropping seasons and compared to the plough-and-harrow, applied after the harvest of irrigated rice in a flat hydromorphic soil in south Brazil. In the subsequent summer, soybean was seeded using a no-tillage seeder. Observations on soybean establishment and grain yield demonstrated that the alternative method performed as well as the plough-based system. Benefits of the roller-based method were a 50% reduction in energy consumption for soil preparation, corresponding to a 22% increase in overall energy use-efficiency of soybean production. Labour time and greenhouse gas emissions for soil preparation were reduced with 29 and 55%, respectively. Next to these savings, the roller-based method can also be performed shortly after rice harvest, creating better opportunities for cover crops or pastures in between rice and soybean. In conclusion, the equivalent agronomic and superior sustainability performance makes the knife-roller method an appealing alternative for seedbed preparation after irrigated rice in lowland production systems.
A lifetime of the Variscan orogenic plateau from uplift to collapse as recorded by the Prague Basin, Bohemian Massif
Authors: VACEK FRANTIŠEK , ŽÁK JIŘÍ
Published: November 2017

The Ordovician to Middle Devonian Prague Basin, Bohemian Massif, represents the shallowest crust of the Variscan orogen corresponding toc.1–4 km palaeodepth. The basin was inverted and multiply deformed during the Late Devonian to early Carboniferous Variscan orogeny, and its structural inventory provides an intriguing record of complex geodynamic processes that led to growth and collapse of a Tibetan-type orogenic plateau. The northeastern part of the Prague Basin is a simple syncline cross-cut by reverse/thrust faults and represents a doubly vergent compressional fan accommodatingc.10–19 % ~NW–SE shortening, only minor syncline axis-parallel extension and significant crustal thickening. The compressional structures were locally overprinted by vertical shortening, kinematically compatible with ductile normal shear zones that exhumed deep crust in the orogen's interior atc. 346–337 Ma. On a larger scale, the deformation history of the Prague Syncline is consistent with building significant palaeoelevation during Variscan plate convergence. Based on a synthesis of finite deformation parameters observed across the upper crust in the centre of the Bohemian Massif, we argue for a differentiated within-plateau palaeotopography consisting of domains of local thickening alternating with topographic depressions over lateral extrusion zones. The plateau growth, involving such complex three-dimensional internal deformations, was terminated by its collapse driven by multiple interlinked processes including gravity, voluminous magma emplacement and thermal softening in the hinterland, and far-field plate-boundary forces resulting from the relative dextral motion of Gondwana and Laurussia.
Phonation and laryngeal specification in American English voiceless obstruents
Authors: Davidson Lisa
Published: November 2017

This study presents a detailed acoustic analysis of phonation in voiceless obstruents in American English (AE) to investigate the acoustic consequences of the laryngeal timing that has been reported in the literature. The current study examines the appearance of phonation in voiceless obstruents in a corpus of read speech with 37 AE speakers. Linguistic factors such as phrase and word position, stress, and the preceding phoneme are examined and are shown to condition the presence and degree of phonation during the constriction period of stops and fricatives. The amount of phonation present is further analyzed by characterizing where in the constriction interval phonation appears. Carryover phonation (or bleed) from a preceding sonorant is most common for stops, while a trough pattern (phonation that dies out and then begins again before the end of the closure) is more prevalent for fricatives. These acoustic patterns, together with previous reports of laryngeal articulation and air pressure measures, have implications for the representation of laryngeal timing in a gestural phonology framework.
Morbid Subjects: Forensic medicine and sovereignty in Siam
Authors: PEARSON QUENTIN (TRAIS)
Published: November 2017

This article examines the question of Siamese sovereignty in the era of high imperialism through the lens of medical jurisprudence. Although Siam (Thailand) was never formally colonized, it was subject to unequal trade treaties that established extraterritorial legal rights for foreign residents. In cases where a foreign resident was suspected of having harmed a Siamese subject, the Siamese state had to appeal to foreign consular officials to file charges against the suspect. Standards of forensic evidence were crucial in such cases. While medical jurisprudence helped to bolster racial privilege in other colonial legal jurisdictions, this article argues that these disputes rendered the dead and injured bodies of Siamese subjects into potentially powerful pieces of leverage against foreign residents and their political representatives. The dead bodies of Siamese subjects became grounds for challenging foreign courts and asserting Siamese sovereignty.
Belgian Rule and its Afterlives: Colonialism, Developmentalism, and Mobutism in the Tanganyika District, Southeastern DR-Congo, 1885–1985
Authors: Loffman Reuben
Published: November 2017

The arrival of Belgian rule in the late nineteenth century initiated significant changes in the labor history of Tanganyika, a province in the southeastern Democratic Republic of the Congo (DRC), as well the discursive regimes used to legitimize these transformations. After the colonial conquests, unfree labor was justified by paternalistic rather than mythical discourses. Although unfree labor was less common in the postcolonial period, the state forced farmers to sell crops at low prices and build roads for no remuneration. In the Cold War context, the language and practice of developmentalism mediated the coercive practices of the independent Congolese state (known as Zaïre, 1971–1997). The floundering Zaïrian government expanded its presence in Tanganyika due to its partnership with USAID. USAID's rhetoric and practice was influenced by a “bottom up” approach to agricultural production, but the cuts to its funding in the 1980s meant it struggled to soften Mobutu's coercive administration.
From Unfree Work to Working for Free: Labor, Aid, and Gender in the Nigerien Sahel, 1930–2000
Authors: Rossi Benedetta
Published: November 2017

This article focuses on the consequences of twentieth-century developmentalism for labor practices in the Nigerien Sahel under French rule and in the postindependence period. It examines labor regime transformations at the desert's edge; the ways in which state-led developmentalism influenced labor relations; and gender disparities in the history of emancipation from slavery. Following the abolition of forced labor in 1946, the rhetoric of human investment was used to promote the “voluntary” participation of workers in colonial development initiatives. This continued under Niger's independent governments. Seyni Kountché’s dictatorship relabeled Niger “Development Society” and mobilized Nigeriens’ “voluntary” work in development projects. Concurrently, drought in the Sahel attracted unprecedented levels of international funding. In the Ader region this led to the establishment of a major antidesertification project that paid local labor on a food-for-work basis. Since most men migrated seasonally to West African cities, the majority of workers in the project's worksites were women who welcomed “project work” to avoid destitution. In the name of development, it continued to be possible to mobilize workers without remuneration beyond the cost of a meal.
A State of Underdevelopment: Sovereignty, Nation-Building and Labor in Liberia 1898–1961
Authors: Whyte Christine
Published: November 2017

In the nineteenth and early twentieth century, Liberia was in the unusual position of being a colony with no metropole. Without military or financial support, the settlers’ control over their territory remained weak. Surrounding European empires preyed on this weakness, and Americo-Liberian rule was often at risk from coalitions of European forces and indigenous African resistance. From the early twentieth century, the political elite took on the concept of “development” as a central part of government policy in an attempt to gain political and economic control of the hinterland areas and stave off European incursions. This policy involved the extension and reinforcement of labor policies and practices that had developed through the nineteenth century as means to incorporate settlers and indigenous people into Liberian society. When these plans failed, huge swathes of territory were turned over to foreign commercial interests in an attempt to bolster Liberian claims to sovereignty. And after the Second World War, new policies of “community development” introduced by international agencies again tried to solve Liberia's “land and labor” problem through resettlement. At each stage developmentalist rationales were deployed in order to facilitate greater government control over the Liberian interior territory.
Design and implementation of a variable stiffness actuator based on flexible gear rack mechanism
Authors: Wang Wei , Fu Xiaoyue , Li Yangmin , Yun Chao
Published: November 2017

Variable stiffness can improve the capability of human–robot interacting. Based on the mechanism of a flexible rack and gear, a rotational joint actuator named vsaFGR is proposed to regulate the joint stiffness. The flexible gear rack can be regarded as a combination of a non-linear elastic element and a linear adjusting mechanism, providing benefits of compactness. The joint stiffness is in the range of 217–3527 N.m/rad, and it is inversely proportional to the 4th-order of the gear displacement, and nearly independent from the joint angular deflection, providing benefits of quick stiffness regulation in a short displacement of 20 mm. The gear displacement with respect to the flexible gear rack is perpendicular to the joint loading force, so the power required for stiffness regulating is as low as 14.4 W, providing benefits of energy saving. The working principles of vsaFGR are elaborated, followed by presentation on the mechanics model and the prototype. The high compactness, great stiffness range and low power cost of vsaFGR are proved by simulations and experiments.
MODEL-BASED COST-EFFECTIVENESS OF CONVENTIONAL AND INNOVATIVE CHEMO-RADIATION IN LUNG CANCER
Authors: Bongers Mathilda L. , de Ruysscher Dirk , Oberije Cary , Lambin Philippe , Uyl-de Groot Carin A. , Belderbos José , Coupé Veerle M.H.
Published: November 2017

Introduction:Optimizing radiotherapy with or without chemotherapy through advanced imaging and accelerated radiation schemes shows promising results in locally advanced non–small-cell lung cancer (NSCLC). This study compared the cost-effectiveness of positron emission tomography-computed tomography based isotoxic accelerated sequential chemo-radiation (SRT2) and concurrent chemo-radiation with daily low-dose cisplatin (CRT2) with standard sequential (SRT1) and concurrent chemo-radiation (CRT1).

Methods:We used an externally validated mathematical model to simulate the four treatment strategies. The model was built using data from 200 NSCLC patients treated with curative sequential chemo-radiation. For concurrent strategies, data from a meta-analysis and a single study were included in the model. Costs, utilities, and resource use estimates were obtained from literature. Primary outcomes were the incremental cost-effectiveness and cost-utility ratio (ICUR) of each strategy. Scenario analyses were carried out to investigate the impact of uncertainty.

Results:Total undiscounted costs and quality-adjusted life-years (QALYs) for SRT1, CRT1, SRT2, and CRT2 were EUR 17,288, EUR 18,756, EUR 19,072, EUR 17,360 and QALYs 1.10, 1.15, 1.40, and 1.40, respectively. Compared with SRT1, the ICURs were EUR 38,024/QALY for CRT1, EUR 6,249/QALY for SRT2, and EUR 346/QALY for CRT2. CRT2 was highly cost-effective compared with SRT1. Moreover, CRT2 was more effective and less costly than CRT1 and SRT2. Therefore, these strategies were dominated by CRT2.

Conclusion:Optimized sequential and concurrent chemo-radiation strategies are more effective and cost-effective than the current conventional sequential and concurrent strategies. Concurrent chemo-radiation with a daily low dose cisplatin regimen is the most cost-effective treatment option for locally advanced inoperable NSCLC patients.
EVALUATION OF PATIENT AND PUBLIC INVOLVEMENT INITIATIVES IN HEALTH TECHNOLOGY ASSESSMENT: A SURVEY OF INTERNATIONAL AGENCIES
Authors: Weeks Laura , Polisena Julie , Scott Anna Mae , Holtorf Anke-Peggy , Staniszewska Sophie , Facey Karen
Published: November 2017

Objectives:Although there is increased awareness of patient and public involvement (PPI) among health technology assessment (HTA) organizations, evaluations of PPI initiatives are relatively scarce. Our objective as members of Health Technology Assessment International's (HTAi's) Patient and Citizen Involvement Group (PCIG) was to advance understanding of the range of evaluation strategies adopted by HTA organizations and their potential usefulness.

Methods:In March 2016, a survey was sent to fifty-four HTA organizations through the International Network of Agencies for Health Technology Assessment (INAHTA) and contacts of members of HTAi's PCIG. Respondents were asked about their organizational structure; how patients and members of the public are involved; whether and how PPI initiatives have been evaluated, and, if so, which facilitators and challenges to evaluation were found and how results were used and disseminated.

Results:Fifteen (n= 15) programs from twelve countries responded (response rate 27.8 percent) that involved patients (14/15) and members of the public (10/15) in HTA activities. Seven programs evaluated their PPI activities, including participant satisfaction (5/7), process (5/7) and impact evaluations (4/7). Evaluation results were used to improve PPI activities, identify education and training needs, and direct strategic priorities. Facilitators and challenges revolved around the need for stakeholder buy-in, sufficient resources, senior leadership, and including patients in evaluations.

Conclusions:A small but diverse set of HTA organizations evaluate their PPI activities using a range of strategies that reflect the range of rationales and approaches to PPI in HTA. It will be important for HTA organizations to draw on evaluation theories and methods.
Distributional learning aids linguistic category formation in school-age children
Authors: HALL Jessica , OWEN VAN HORNE Amanda , FARMER Thomas
Published: November 2017

The goal of this study was to determine if typically developing children could form grammatical categories from distributional information alone. Twenty-seven children aged six to nine listened to an artificial grammar which contained strategic gaps in its distribution. At test, we compared how children rated novel sentences that fit the grammar to sentences that were ungrammatical. Sentences could be distinguished only through the formation of categories of words with shared distributional properties. Children's ratings revealed that they could discriminate grammatical and ungrammatical sentences. These data lend support to the hypothesis that distributional learning is a potential mechanism for learning grammatical categories in a first language.
A note on the polynomial Freĭman–Ruzsa conjecture over ℤ
Authors: MANNERS FREDDIE
Published: November 2017

The polynomial Freĭman–Ruzsa conjecture over the integers is often phrased in terms of convex progressions. We give an alternative, apparently stronger formulation in terms of the more restrictive “ellipsoid progressions”, and show that these formulations are in fact equivalent. The key input to the equivalence proof comes from strong results in asymptotic convex geometry.
Sustainability impact assessments of different urban short food supply chains: examples from London, UK
Authors: Schmutz Ulrich , Kneafsey Moya , Sarrouy Kay Carla , Doernberg Alexandra , Zasada Ingo
Published: November 2017

Greater London has a vibrant food scene comprising of many different types of urban and peri-urban ‘short food supply chains’ (SFSCs). This paper reports on exploratory research, which used examples of SFSCs from London to build a more detailed understanding of different types of urban SFSC and their relative performance compared to each other. To do this, we undertook a participatory sustainability impact assessment (SIA) in which local food system stakeholders were asked to rank the perceived impacts of five different urban and peri-urban SFSCs compared with the current ‘mainstream’ food supply system (defined as supermarkets retailing mass-produced, standardized food products). The SFSCs ranked were: (1) urban gardening (self-supply), (2) urban gardening (commercial), (3) community supported agriculture (CSA), (4) direct sale (on-farm) and (5) direct sale (off-farm). Results from the SIA showed that CSAs were regarded as delivering the highest overall social, economic and environmental benefits, followed by urban gardening (commercial), urban gardening (self-supply) and direct sales (off-farm). The lowest overall rating was for the supply chain direct sales (on-farm). All five SFSCs were ranked highest on the social aspects of sustainability. Following the participatory SIA of perceived sustainability, we next developed a questionnaire in order to test the feasibility of measuring the specific (rather than ‘perceived’) impacts of an urban SFSC. We applied the test at a community-led local food market in South London, where we conducted face-to-face interviews with all market stallholders (18 respondents) and a random sample of consumers (51 respondents). Results from the community-led market were similar to those acquired at the SIA workshop, with greater agreement about the social benefits of the market, compared with economic and environmental impacts. The results also suggest that producers underestimated consumers’ willingness to share the risks that the producers face and we identify this as an important aspect for future research. The paper concludes with reflections on the strengths and weaknesses of the SIA methodology.
Tackling IUU Fishing: Developing a Holistic Legal Response
Authors: Soyer Barış , Leloudas George , Miller Dana
Published: November 2017

Illegal, unreported and unregulated (IUU) fishing is a global problem, which threatens marine ecosystems in addition to putting food security and regional stability at risk. It is often linked to major human rights violations and even organized crime. Legal measures, such as introducing monitoring and surveillance systems or denying services to vessels engaged in IUU fishing, are often implemented at national and international levels to combat such practices. Academics and economists have suggested that IUU fishing might be discouraged equally well by taking the profit out of it. Building on this premise, this article analyzes the extent to which the availability of liability insurance contributes to the problem of IUU fishing. To this end, an empirical study has been carried out, which supports the contention that vessels suspected of involvement in IUU fishing have no serious difficulty in obtaining liability insurance from the market and insurance sector, thereby inadvertently facilitating IUU fishing. The authors conclude that to deter IUU fishing, access to insurance for those involved in it should be restricted. Some success can be achieved if certain steps are taken to improve the risk assessment procedures of underwriters. However, it is advocated that the most effective approach would be the reform of European Union or domestic legislation and putting providers of liability insurance under a clear positive obligation to refuse cover to those involved in IUU fishing.
Effect of Tillage Systems on the Dissipation of Prosulfocarb Herbicide
Authors: Scherner Ananda , Schreiber Fabio , Fomsgaard Inge S. , Melander Bo , Avila Luis A. , Kudsk Per
Published: November 2017

Crop management practices such as tillage can influence the dissipation of herbicides in soil. This study aimed to determine the effects of tillage systems on soil dissipation of prosulfocarb (PSC) using two assessment methods: bioassay and high-performance liquid chromatography (HPLC) analysis. PSC was applied on plots cultivated under three tillage systems (moldboard plowing, tine tillage at 8- to 10-cm soil depth, and direct drilling) at different rates (0, 500, 1,000, 2,000, 4,000 and 8,000 g ai ha−1) and two spraying times, representing early and late sowing time of winter cereals in Denmark. The experiment was conducted over 2 yr. The soil was analyzed for PSC residues by HPLC and a bioassay, using silky windgrass as the indicator plant. Neither technique revealed an effect of tillage systems on PSC dissipation, but the LD50values estimated based on the bioassays were generally lower under direct drilling (11.7 d) than with plowing (17.5 d). Moreover, LD50estimates based on bioassay results were generally lower than those estimated with HPLC analyses. Half-life values estimated with HPLC were low and not within the range of values reported in the literature (ca. 20 d), suggesting enhanced degradation of PSC. In addition to influencing the performance of PSC against problematic weed species, an enhanced dissipation rate could also hamper the benefits of PSC in an antiresistance strategy.
UAV Low-Altitude Remote Sensing for Precision Weed Management
Authors: Huang Yanbo , Reddy Krishna N. , Fletcher Reginald S. , Pennington Dean
Published: November 2017

Precision weed management, an application of precision agriculture, accounts for within-field variability of weed infestation and herbicide damage. Unmanned aerial vehicles (UAVs) provide a unique platform for remote sensing of field crops. They are more efficient and flexible than manned agricultural airplanes in acquiring high-resolution images at low altitudes and low speeds. UAVs are more universal than agricultural aircraft, because the latter are used only in specific regions. We have developed and used UAV systems for red–green–blue digital and color–infrared imaging over crop fields to identify weed species, determine crop injury from dicamba at different doses, and detect naturally grown glyphosate-resistant weeds. This article presents remote sensing technologies for weed management and focuses on development and application of UAV-based low-altitude remote sensing technology for precision weed management. In particular, this article futher discusses the potential application of UAV-based plant-sensing systems for mapping the distributions of glyphosate-resistant and glyphosate-susceptible weeds in crop fields.
Just Don’t Call it a Tax! Framing in an Experiment on Voting and Redistribution
Authors: Lorenz Jan , Paetzel Fabian , Tepe Markus
Published: November 2017

Utilizing a simplified version of the Meltzer–Richard redistribution mechanism, we designed a laboratory experiment to test whether it matters if voters were asked to decide on a tax rate or a minimum income, leaving the redistribution mechanism itself unchanged. Framing the vote about redistribution as a decision about a minimal income increases the individually and ideally preferred level of redistribution. This effect outlives the groups’ deliberation processes and leads to the implementation of a higher level of redistribution.
Status Quo Bias in Ballot Wording
Authors: Barber Michael , Gordon David , Hill Ryan , Price Joseph
Published: November 2017

We examine the role of status quo bias in the ballot wording of social issues that affect the rights of minority groups. We test the salience of this framing bias by conducting an experiment that randomly assigns different ballot wordings for five policies across survey respondents. We find that status quo bias changes the percent of individuals who vote for the ballot measure by 5–8 percentage points with the least informed individuals being the most affected by status quo bias.
Analog Modeling Recreates Millions of Years in a Few Hours
Authors: Reber Jacqueline , Dooley Tim , Logan Elizabeth
Published: November 2017

Second Workshop on Analog Modeling of Tectonic Processes; Austin, Texas, 17–19 May 2017
Fbxo4-mediated degradation of Fxr1 suppresses tumorigenesis in head and neck squamous cell carcinoma
Authors: Qie Shuo , Majumder Mrinmoyee , Mackiewicz Katarzyna , Howley Breege V. , Peterson Yuri K. , Howe Philip H. , Palanisamy Viswanathan , Diehl J. Alan
Published: November 2017

The Fbxo4 tumour suppressor is a component of an Skp1-Cul1-F-box E3 ligase for which two substrates are known. Here we show purification of SCFFbxo4 complexes results in the identification of fragile X protein family (FMRP, Fxr1 and Fxr2) as binding partners. Biochemical and functional analyses reveal that Fxr1 is a direct substrate of SCFFbxo4. Consistent with a substrate relationship, Fxr1 is overexpressed in Fbxo4 knockout cells, tissues and in human cancer cells, harbouring inactivating Fbxo4 mutations. Critically, in head and neck squamous cell carcinoma, Fxr1 overexpression correlates with reduced Fbxo4 levels in the absence of mutations or loss of mRNA, suggesting the potential for feedback regulation. Direct analysis reveals that Fbxo4 translation is attenuated by Fxr1, indicating the existence of a feedback loop that contributes to Fxr1 overexpression and the loss of Fbxo4. Ultimately, the consequence of Fxr1 overexpression is the bypass of senescence and neoplastic progression.
Genome-driven evolutionary game theory helps understand the rise of metabolic interdependencies in microbial communities
Authors: Zomorrodi Ali R. , Segrè Daniel
Published: November 2017

Metabolite exchanges in microbial communities give rise to ecological interactions that govern ecosystem diversity and stability. It is unclear, however, how the rise of these interactions varies across metabolites and organisms. Here we address this question by integrating genome-scale models of metabolism with evolutionary game theory. Specifically, we use microbial fitness values estimated by metabolic models to infer evolutionarily stable interactions in multi-species microbial “games”. We first validate our approach using a well-characterized yeast cheater-cooperator system. We next perform over 80,000 in silico experiments to infer how metabolic interdependencies mediated by amino acid leakage in Escherichia coli vary across 189 amino acid pairs. While most pairs display shared patterns of inter-species interactions, multiple deviations are caused by pleiotropy and epistasis in metabolism. Furthermore, simulated invasion experiments reveal possible paths to obligate cross-feeding. Our study provides genomically driven insight into the rise of ecological interactions, with implications for microbiome research and synthetic ecology.
Excavating hidden adsorption sites in metal-organic frameworks using rational defect engineering
Authors: Chong Sanggyu , Thiele Günther , Kim Jihan
Published: November 2017

Metal–organic frameworks are known to contain defects within their crystalline structures. Successful engineering of these defects can lead to modifications in material properties that can potentially improve the performance of many existing frameworks. Herein, we report the high-throughput computational screening of a large experimental metal–organic framework database to identify 13 frameworks that show significantly improved methane storage capacities with linker vacancy defects. The candidates are first identified by focusing on structures with methane-inaccessible pores blocked away from the main adsorption channels. Then, organic linkers of the candidate structures are judiciously replaced with appropriate modulators to emulate the presence of linker vacancies, resulting in the integration and utilization of the previously inaccessible pores. Grand canonical Monte Carlo simulations of defective candidate frameworks show significant enhancements in methane storage capacities, highlighting that rational defect engineering can be an effective method to significantly improve the performance of the existing metal–organic frameworks.
Drip tectonics and the enigmatic uplift of the Central Anatolian Plateau
Authors: Göğüş Oğuz H. , Pysklywec Russell N. , Şengör A. M. C. , Gün Erkan
Published: November 2017

Lithospheric drips have been interpreted for various regions around the globe to account for the recycling of the continental lithosphere and rapid plateau uplift. However, the validity of such hypothesis is not well documented in the context of geological, geophysical and petrological observations that are tested against geodynamical models. Here we propose that the folding of the Central Anatolian (Kırşehir) arc led to thickening of the lithosphere and onset of “dripping” of the arc root. Our geodynamic model explains the seismic data showing missing lithosphere and a remnant structure characteristic of a dripping arc root, as well as enigmatic >1 km uplift over the entire plateau, Cappadocia and Galatia volcanism at the southern and northern plateau margins since ~10 Ma, respectively. Models show that arc root removal yields initial surface subsidence that inverts >1 km of uplift as the vertical loading and crustal deformation change during drip evolution.
Backward Cherenkov radiation emitted by polariton solitons in a microcavity wire
Authors: Skryabin D. V. , Kartashov Y. V. , Egorov O. A. , Sich M. , Chana J. K. , Tapia Rodriguez L. E. , Walker P. M. , Clarke E. , Royall B. , Skolnick M. S. , Krizhanovskii D. N.
Published: November 2017

Exciton-polaritons in semiconductor microcavities form a highly nonlinear platform to study a variety of effects interfacing optical, condensed matter, quantum and statistical physics. We show that the complex polariton patterns generated by picosecond pulses in microcavity wire waveguides can be understood as the Cherenkov radiation emitted by bright polariton solitons, which is enabled by the unique microcavity polariton dispersion, which has momentum intervals with positive and negative group velocities. Unlike in optical fibres and semiconductor waveguides, we observe that the microcavity wire Cherenkov radiation is predominantly emitted with negative group velocity and therefore propagates backwards relative to the propagation direction of the emitting soliton. We have developed a theory of the microcavity wire polariton solitons and of their Cherenkov radiation and conducted a series of experiments, where we have measured polariton-soliton pulse compression, pulse breaking and emission of the backward Cherenkov radiation.
A soft selective sweep during rapid evolution of gentle behaviour in an Africanized honeybee
Authors: Avalos Arian , Pan Hailin , Li Cai , Acevedo-Gonzalez Jenny P. , Rendon Gloria , Fields Christopher J. , Brown Patrick J. , Giray Tugrul , Robinson Gene E. , Hudson Matthew E. , Zhang Guojie
Published: November 2017

Highly aggressive Africanized honeybees (AHB) invaded Puerto Rico (PR) in 1994, displacing gentle European honeybees (EHB) in many locations. Gentle AHB (gAHB), unknown anywhere else in the world, subsequently evolved on the island within a few generations. Here we sequence whole genomes from gAHB and EHB populations, as well as a North American AHB population, a likely source of the founder AHB on PR. We show that gAHB retains high levels of genetic diversity after evolution of gentle behaviour, despite selection on standing variation. We observe multiple genomic loci with significant signatures of selection. Rapid evolution during colonization of novel habitats can generate major changes to characteristics such as morphological or colouration traits, usually controlled by one or more major genetic loci. Here we describe a soft selective sweep, acting at multiple loci across the genome, that occurred during, and may have mediated, the rapid evolution of a behavioural trait.
Oxidation behavior of graphene-coated copper at intrinsic graphene defects of different origins
Authors: Kwak Jinsung , Jo Yongsu , Park Soon-Dong , Kim Na Yeon , Kim Se-Yang , Shin Hyung-Joon , Lee Zonghoon , Kim Sung Youb , Kwon Soon-Yong
Published: November 2017

The development of ultrathin barrier films is vital to the advanced semiconductor industry. Graphene appears to hold promise as a protective coating; however, the polycrystalline and defective nature of engineered graphene hinders its practical applications. Here, we investigate the oxidation behavior of graphene-coated Cu foils at intrinsic graphene defects of different origins. Macro-scale information regarding the spatial distribution and oxidation resistance of various graphene defects is readily obtained using optical and electron microscopies after the hot-plate annealing. The controlled oxidation experiments reveal that the degree of structural deficiency is strongly dependent on the origins of the structural defects, the crystallographic orientations of the underlying Cu grains, the growth conditions of graphene, and the kinetics of the graphene growth. The obtained experimental and theoretical results show that oxygen radicals, decomposed from water molecules in ambient air, are effectively inverted at Stone–Wales defects into the graphene/Cu interface with the assistance of facilitators.
Height associated variants demonstrate assortative mating in human populations
Authors: Li Xiaoyin , Redline Susan , Zhang Xiang , Williams Scott , Zhu Xiaofeng
Published: November 2017

Understanding human mating patterns, which can affect population genetic structure, is important for correctly modeling populations and performing genetic association studies. Prior studies of assortative mating in humans focused on trait similarity among spouses and relatives via phenotypic correlations. Limited research has quantified the genetic consequences of assortative mating. The degree to which the non-random mating influences genetic architecture remains unclear. Here, we studied genetic variants associated with human height to assess the degree of height-related assortative mating in European-American and African-American populations. We compared the inbreeding coefficient estimated using known height associated variants with that calculated from frequency matched sets of random variants. We observed significantly higher inbreeding coefficients for the height associated variants than from frequency matched random variants (P < 0.05), demonstrating height-related assortative mating in both populations.
Identification of host DEAD-box RNA helicases that regulate cellular tropism of oncolytic Myxoma virus in human cancer cells
Authors: Rahman Masmudur M. , Bagdassarian Eugenie , Ali Mohamed A. M. , McFadden Grant
Published: November 2017

Myxoma virus (MYXV), a Leporipoxvirus, is being developed as an oncolytic virotherapeutic for the treatment of a variety of human cancers. MYXV tropism for human cancer cells is largely mediated by intracellular signaling networks that regulate viral replication or innate antiviral response pathways. Thus, MYXV is fully or partially permissive for the majority of human cancer cells that harbor defects in antiviral signaling, but a minority are nonpermissive because the virus infection aborts before its completion. To identify host factors relevant for MYXV tropism in human cancer cells, we performed a small interfering RNA (siRNA) library screen targeting the 58 human DEAD-box RNA helicases in two permissive human cancer cells (HeLa and A549), one semi-permissive (786-0), and one nonpermissive cell line (PANC-1). Five host RNA helicases (DDX3X, DDX5, DHX9, DHX37, DDX52) were inhibitory for optimal replication and thus classified as anti-viral, while three other cellular RNA helicases (DHX29, DHX35, RIG-I) were identified as pro-viral or pro-cellular because knockdown consistently reduced MYXV replication and/or required metabolic functions of permissive cancer cells. These findings suggest that replication of MYXV, and likely all poxviruses, is dramatically regulated positively and negatively by multiple host DEAD-box RNA helicases.
Dose de-escalation to the normal larynx using conformal radiotherapy reduces toxicity while maintaining oncologic outcome for T1/T2 glottic cancer
Authors: Kim Jun Won , Byeon Hyung Kwon , Choi Hong-Shik , Lee Ik Jae
Published: November 2017

We evaluated the efficacy of dose de-escalation to the normal larynx using conformal radiotherapy (CRT) for T1/T2 glottic cancer. For conventional RT (2DRT, n = 38), the laryngeal box received a median equivalent dose in 2 Gy fractions (EQD2) of 66 Gy. For CRT (n = 42; 3D-CRT, 20; intensity-modulated RT, 22), clinical target volume (CTV)1 (gross tumor with a 3-mm margin) and CTV2 (laryngeal box) received median EQD2s of 66.6 Gy and 52.2 Gy, respectively. With a 71-month median follow-up, five-year local control and overall survival rates for 2DRT vs. CRT were 88.1% vs. 95.1% (p = 0.405) and 94.7% vs. 90.7% (p = 0.102), respectively. Grade 2 and 3 pharyngitis rates were 52.6% and 5.3% for 2DRT vs. 35.7% and 2.4% for CRT (p = 0.121). Grade 2 and 3 dermatitis rates were 42.1% and 2.6% for 2DRT vs. 35.7% and 0% for CRT (p = 0.013). The maximum phonation time increased from 12.1 ± 7.1 to 14.0 ± 6.6 seconds after 2DRT (p = 0.375) and from 12.0 ± 5.5 to 13.8 ± 10.1 seconds after CRT (p = 0.313). Fundamental frequency decreased from 150.6 ± 40.3 to 121.9 ± 30.2 Hz after 2DRT (p = 0.039) and from 138.5 ± 31.9 to 126.1 ± 23.7 Hz after CRT (p = 0.058). CRT can effectively de-escalate the normal larynx dose while maintaining oncologic outcome and voice quality.
Trapping/Pinning of colloidal microspheres over glass substrate using surface features
Authors: Prakash Praneet , Varma Manoj
Published: November 2017

Suspensions of micro/nano particles made of Polystyrene, Poly(methyl methacrylate), Silicon dioxide etc. have been a standard model system to understand colloidal physics. These systems have proved useful insights into phenomena such as self-assembly. Colloidal model systems are also extensively used to simulate many condensed matter phenomena such as dynamics in a quenched disordered system and glass transition. A precise control of particles using optical or holographic tweezers is essential for such studies. However, studies of collective phenomena such as jamming and flocking behaviour in a disordered space are limited due to the low throughput of the optical trapping techniques. In this article, we present a technique where we trap and pin polystyrene microspheres ~10 μm over ‘triangular crest’ shaped microstructures in a microfluidic environment. Trapping/Pinning occurs due to the combined effect of hydrodynamic interaction and non-specific adhesion forces. This method allows trapping and pinning of microspheres in any arbitrary pattern with a high degree of spatial accuracy which can be useful in studying fundamentals of various collective phenomena as well as in applications such as bead detachment assay based biosensors.
Electrical analysis of c-Si/CGSe monolithic tandem solar cells by using a cell-selective light absorption scheme
Authors: Jeong Ah Reum , Choi Sung Bin , Kim Won Mok , Park Jong-Keuk , Choi Jihye , Kim Inho , Jeong Jeung-hyun
Published: November 2017

A monolithic tandem solar cell consisting of crystalline Si (c-Si)/indium tin oxide (ITO)/CuGaSe2(CGSe) was demonstrated by stacking a CGSe solar cell on a c-Si/ITO solar cell to obtain a photovoltaic conversion efficiency of about 10%. Electrical analyses based on cell-selective light absorption were applied to individually characterize the photovoltaic performances of the top and bottom subcells. Illumination at a frequency that could be absorbed only by a targeted top or bottom subcell permitted measurement of the open-circuit voltage of the target subcell and the shunt resistance of the non-target subcell. The cell parameters measured from each subcell were very similar to those of the corresponding single cell, confirming the validity of the suggested method. In addition, separating the light absorption intensities at the top and bottom subcells made us measure the bias-dependent photocurrent for each subcell. The series resistance of a c-Si/ITO/CGSe cell subjected to bottom-cell limiting conditions was slightly large, implying that the tunnel junction was a little resistive or slightly beyond ohmic. This analysis demonstrated that aside from producing a slightly resistive tunnel junction, our fabrication processes were successful in monolithically integrating a CGSe cell onto a c-Si/ITO cell without degrading the performances of both cells.
Real-time wash-free detection of unlabeled PNA-DNA hybridization using discrete FET sensor
Authors: Kaisti Matti , Kerko Anssi , Aarikka Eero , Saviranta Petri , Boeva Zhanna , Soukka Tero , Lehmusvuori Ari
Published: November 2017

We demonstrate an electrochemical sensor for detection of unlabeled single-stranded DNA using peptide nucleic acid (PNA) probes coupled to the field-effect transistor (FET) gate. The label-free detection relies on the intrinsic charge of the DNA backbone. Similar detection schemes have mainly concentrated on sensitivity improvement with an emphasis on new sensor structures. Our approach focuses on using an extended-gate that separates the FET and the sensing electrode yielding a simple and mass fabricable device. We used PNA probes for efficient hybridization in low salt conditions that is required to avoid the counter ion screening. As a result, significant part of the target DNA lies within the screening length of the sensor. With this, we achieved a wash-free detection where  typical gate potential shifts are more than 70 mV with 1 µM target DNA. We routinely obtained a real-time, label- and wash-free specific detection of target DNA in nanomolar concentration with low-cost electronics and the responses were achieved within minutes after introducing targets to the solution. Furthermore, the results suggest that the sensor performance is limited by specificity rather than by sensitivity and using low-cost electronics does not limit the sensor performance in the presented sensor configuration.
Coupled Motion of Contact Line on Nanoscale Chemically Heterogeneous Surfaces for Improved Bubble Dynamics in Boiling
Authors: Jaikumar Arvind , Kandlikar Satish G.
Published: November 2017

We demonstrate that the contact line (CL) motion on energetically heterogeneous solid surfaces occurs in a coupled fashion as against the traditional staggered stick-slip motion. Introducing chemical inhomogeneities at nanoscale induces a local change in dynamic contact angles which manifests as a smooth and continuous motion of the CL. Nanoscale chemically inhomogeneous surfaces comprising of gold, palladium and nickel were generated on copper substrates to demonstrate the underlying CL dynamics. The spatial variations of chemical constituents were mapped using elemental display scanning electron microscope images. Further, the coupled and stick-slip motion was confirmed for a sliding water droplet on these surfaces, and then used in studying the pool boiling bubble dynamics of a single bubble from nucleation to departure. The coupled motion was seen to increase the CL velocity thereby increasing the contribution from transient conduction heat transfer. Consequently, a ~2X increase in the boiling critical heat flux (CHF) was observed. Enhancing the pool boiling performance by introducing nanoscale surface features is an attractive approach in many applications and this work provides a framework and understanding of the CL motion induced through the chemical inhomogeneity effects.
Disturbance in Plasma Metabolic Profile in Different Types of Human Cytomegalovirus-Induced Liver Injury in Infants
Authors: Li Wei-Wei , Shan Jin-Jun , Lin Li-Li , Xie Tong , He Li-Li , Yang Yan , Wang Shou-Chuan
Published: November 2017

Human cytomegalovirus (HCMV) infection in infants is a global problem and the liver is a target organ of HCMV invasion. However, the mechanism by which HCMV causes different types of liver injury is unclear, and there are many difficulties in the differential diagnosis of HCMV infantile cholestatic hepatopathy (ICH) and extrahepatic biliary atresia (EHBA). We established a non-targeted gas chromatography-mass spectrometry metabolomics method in conjunction with orthogonal partial least squares-discriminate analysis based on 127 plasma samples from healthy controls, and patients with HCMV infantile hepatitis, HCMV ICH, and HCMV EHBA to explore the metabolite profile of different types of HCMV-induced liver injury. Twenty-nine metabolites related to multiple amino acid metabolism disorder, nitrogen metabolism and energy metabolism were identified. Carbamic acid, glutamate, L-aspartic acid, L-homoserine, and noradrenaline for HCMV ICH vs. HCMV EHBA were screened as potential biomarkers and showed excellent discriminant performance. These results not only revealed the potential pathogenesis of HCMV-induced liver injury, but also provided a feasible diagnostic tool for distinguishing EHBA from ICH.