bluuebunny/crossref_metadata_2025 huggingface dataset:
Генераторы квантовых одномерных диффузий
Authors: Holevo Alexander Semenovich
Published: April 2019

Квантовые динамические полугруппы являются некоммутативным аналогом (суб)-марковских полугрупп в классической теории вероятностей: последние являются полугруппами отображений в функциональных пространствах, тогда как первые - полугруппами отображений в операторных алгебрах, имеющими определенные свойства положительности и нормировки. В этой статье мы описываем квантовые динамические полугруппы, которые являются некоммутативным аналогом классических диффузий на $\mathbf{R}$ и $\mathbf{R}_+$ и демонстрируем различные свойства этих полугрупп в зависимости от граничного условия. Мы также даем доказательство результата, описывающего область определения генератора "некоммутативной диффузии на $\mathbf{R}_+$ с исчезновением в 0" и приводим явный пример ядерного оператора в этой области, который не принадлежит области определения замыкания исходного оператора.
The effect of verbal agreement marking on the use of null and overt subjects: a quantitative study of first person singular in Brazilian Portuguese
Authors: Soares Eduardo Correa , Miller Philip H. , Hemforth Barbara
Published: April 2019

Em um estudo de corpus e em dois experimentos de aceitabilidade, investigamos se existe uma preferência por sujeitos nulos ou pronominais com verbos ambíguos (sincréticos) ou exclusivos (não-ambíguos) no Português Brasileiro (PB). A literatura propõe que o PB (i) está desativando o “Princípio Evitar Pronome” e (ii) é uma língua parcialmente “pro-drop”, cujos contextos morfossintáticos para sujeitos nulos são restritos. Nosso estudo de corpus e os experimentos de aceitabilidade mostram que há um efeito do sincretismo de pessoas no paradigma verbal sobre a frequência relativa de sujeitos nulos e sobre a aceitabilidade de sentenças com sujeitos nulos na primeira pessoa do singular: há uma tendência a evitar ambiguidade para sujeitos nulos com formas verbais ambíguas, mas apenas em contextos em que há competição de antecedentes. Esses resultados são analisados à luz de uma teoria geral da resolução da anáfora (Ariel 1990, etc.), como resultado de um cálculo levando em conta a acessibilidade dos antecedentes potenciais e o custo da forma anafórica (ALMOR, 1996).
O discurso sobre saúde no programa “Bem Estar” à luz das categorias de consciência possível e criação cultural
Authors: Chaves Pombo Alan Cesar Dantas , Malina André , De Azevedo Ângela Celeste Barreto
Published: April 2019

O presente artigo foi realizado a partir de uma pesquisa sobre um programa de TV de tipo mass media. Do ponto de vista teórico-metodológico, utilizou-se as categorias de consciência possível e criação cultural de Lucien Goldmann para verificar como o discurso sobre saúde e estilo de vida está sendo veiculado nas falas de apresentadores e convidados. Os principais resultados mostraram um eixo norteador de direcionamento discursivo para determinação de comportamentos sociais e, ao mesmo tempo, antagonismo às características da consciência possível e da criação cultural da população que assiste a tal programa de TV.

 

ABSTRACT:

This article was based on a research on a mass media TV program. From the theoretical and methodological point of view, Lucien Goldmann's categories of possible consciousness and cultural creation were used to verify how the discourse on health and lifestyle is being conveyed in the speeches of presenters and guests in the TV show. The main results showed a guiding axis of discursive direction for determination of social behaviors and, at the same time, antagonism to the characteristics of the possible consciousness and the cultural creation of the population that attends such TV program.

 

RESUMEN:

El presente artículo se realizó a partir de una investigación sobre un programa de televisión de tipo mass media. Desde el punto de vista teórico-metodológico, se utilizaron las categorías de conciencia posible y creación cultural de Lucien Goldmann para verificar cómo el discurso sobre salud y estilo de vida está siendo vehiculado en las conversaciones de presentadores e invitados. Los principales resultados mostraron un eje orientador de direccionamiento discursivo para la determinación de comportamientos sociales y, al mismo tiempo, antagonismo a las características de la conciencia posible y de la creación cultural de la población que asiste a tal programa de televisión.
ENERGY ANALYSIS OF THE USE PHASE OF CONVENTIONAL TIRES COMPARED TO GUAYULE NR TIRES
Authors: Theregowda Ranjani B. , Eranki Pragnya L. , Landis Amy E.
Published: April 2019

Guayule NR can be grown in the United States and offers a potentially more secure and sustainable alternative to the substantial Hevea NR that is imported from Southeast Asia. This paper presents the first rolling resistance and use-phase energy consumption estimates for guayule tires. The results of this study show that use-phase life cycle energy reductions can be achieved with NRs and that the rolling resistance coefficient (RRcnew) and reference service life (RSL) of the new tire are the critical parameters that pertain to energy and fuel efficiency. A tire's use phase accounts for approximately 86% of its life cycle energy consumption and thus is an important consideration in sustainability assessments. We calculated the use-phase energy consumption for two types of NR tires: a 100% guayule rubber tire and an experimental epoxidized NR tire. These two NR tires were compared against a conventional passenger tire made by Cooper Tire & Rubber Company. The ISO product category rules for passenger tires were used to determine energy consumption, while relevant data were measured from concept tires built in collaboration with Cooper Tire & Rubber Company. The results of this analysis showed that both experimental NR tires have improved use-phase energy consumption compared to conventional rubber tires. Although the epoxidized NR tire with low RRcnew consumes the least energy during the use phase, it is currently not being considered for commercial manufacture. The 100% guayule rubber tire shows a 6% reduction in energy consumption compared to the conventional rubber tire. Results of the sensitivity analysis showed a linear change in use-phase energy consumption with the relevant tire load and RRcnew and an inverse change with the average vehicle fuel efficiency and the RSL.
The Potential Impact and Influence of Private Higher Education Institutions in the Internationalization of Higher Education in South Africa
Authors: Ramlachan Prem
Published: April 2019

The United Nations Sustainable Development Goal 4 advocates the need for inclusive and equitable quality education, which will promote life-long learning opportunities for all. Globally, however, the demand for access to higher education exceeds the places which are available, resulting in many students who cannot obtain places in institutions and in their respective programmes of choice.A case study research method was utilized, using a private higher education institution (HEI), referred to here as “Institution Y”. The empirical part of the search focused on engaging in dialogue with some of the directors based on their experience by retrieving information around the internationalisation of private higher education.South Africa, in its quest to be the preferred destination of choice to study and research, has to discover a working basis that is universal in its application. Arguments influencing inclusivity, equality and life-long learning focuses around conceptualisation, foot-printing and agility, surfaced in the study.The potential impact and influence of private HEIs on internationalisation of higher education can only be aligned with Goal 4 if re-conceptualisation, increasing the international footprint and agility, is driven aggressively for the greater good of the South African higher education.It is advocated, amongst other recommendations, that private and public higher educations be strengthened systemically in South Africa, so that it becomes the destination of choice for higher education to realise Sustainable Development Goal 4.
Research on Cultural Introduction Model in College English Teaching
Authors: Mingjie Bao
Published: April 2019

Language and culture interact and restrict each other, and those two are inseparable. In college English teaching, the teaching quality and students’ cultural knowledge and cultivation have been greatly improved and enhanced with the help of introducing cultural background knowledge of western countries, cultural connotation of vocabulary and cultural differences between China and Western countries. It is now available to leverage information technology platform, proceed from students’ reading and writing perspective, and orient by humanistic thinking in teaching process. Those overall will contribute to the organic integration of language learning and cultural learning and lead to the new direction of college English teaching.
The Hybrid Course for College-Level Elementary Korean: Its Development and Implementation
Authors: Yong Namseok , Ko Seongyeon
Published: April 2019

The past decades have witnessed a surge of scholarly interest in the development of viable foreign language (FL) curricula that integrate various instructional technologies into traditional face-to-face language instruction. This is because its proper application has proved effective, especially in helping students facing temporal and/or geographical limitations and in motivating students with different learning abilities to engage more actively in various learning tasks (Hampel & Stickler, 2015; Meskill & Anthony, 2015). This article aims to contribute to the needs of KFL educators who want to apply modern instructional technologies to their classroom-only-based teaching/learning environments. We showcase the elementary-level Korean courses (KOREAN 101 and 102) in their hybrid format, built upon the “flipped” classroom learning model at Queens College of the City University of New York.
Linking Language Learning with Community: A Community-Based Learning Project for Advanced Korean Courses
Authors: Lee-Smith Angela
Published: April 2019

This article focuses on the significance of community-based learning (CBL), which has yet to be incorporated more widely and proactively in the field of second language education, including Korean. It introduces the Junior-and-Mentor (JAM) project as a model CBL project for advanced language courses, describes its outcomes, and discusses pedagogical implications. The article concludes with a suggestion for collaboration on expansion of the CBL project repertoires and creation of a CBL project repository for the language-teaching community.
Educação infantil: considerações sobre a formação de professores
Authors: Côco Valdete
Published: April 2019

Este artigo tematiza a formação de professores, em vinculação com pesquisa que vem acompanhando a educação infantil na formação inicial, com o objetivo de observar seu posicionamento no horizonte das escolhas profissionais dos licenciandos do Curso de Pedagogia, em interface com a configuração processual desse campo de trabalho. Na perspectiva de pesquisa qualitativa do tipo exploratória, observando assertivas ligadas ao estudo documental, com referenciais bakhtinianos, aborda as proposições relativas à formação no quadro da aprovação das novas diretrizes (BRASIL, 2015a, 2015b). Para tratar da formação de professores no contexto da implementação das novas diretrizes, parte dos desafios da educação infantil (apontados pela literatura) para focalizar a vinculação com o Plano Nacional de Educação (BRASIL, 2014), a proposição de organicidade nas ações de formação e as menções à educação infantil. Como resultados, evidencia os desafios, conexos à formação, que marcam a primeira etapa da educação básica, assinalando o reconhecimento de um campo de trabalho que precisa avançar nos investimentos dirigidos à conquista de quadros funcionais. No propósito de superar as fragilidades no desenvolvimento da profissionalidade docente, advoga que a formação é uma pauta que não pode ser distanciada dos demais desafios que marcam a trajetória da educação infantil, em especial os relativos à configuração das carreiras e às condições de trabalho. Na premissa da formação associada à valorização profissional, afirmada pelas novas diretrizes, outras iniciativas emergem como possibilidades, requerendo avançar na luta por conquistas que respondam aos desafios que persistem nesse campo de trabalho.
Wettelijke maximering van de vertrekvergoeding onder de Wbfo: géén verplichting voor de rechter tot ambtshalve toepassing
Authors: Helstone A.M.
Published: April 2019

Wettelijke maximering van de vertrekvergoeding onder de Wbfo: géén verplichting voor de rechter tot ambtshalve toepassing

Op 1 juni 2018 (ECLI:NL:HR:2018:818) heeft de Hoge Raad zich voor het eerst uitgelaten over de procesrechtelijke status van het wettelijk maximum voor de vertrekvergoeding van bestuurders ex artikel 1:125 lid 2 Wft. Volgens de Hoge Raad is deze bepaling geen regel van openbare orde die ex artikel 25 Rv ambtshalve door de rechter zou moeten worden toegepast. In deze annotatie worden de relevante gezichtspunten voor de verplichting tot ambtshalve toepassing ex artikel 25 Rv geanalyseerd. Tevens wordt stilgestaan bij de oorsprong en de doelstellingen van de beloningsnormen van de Wft en de Europese regels. Hiermee wordt beoogd een bijdrage te leveren aan de bredere gedachtevorming over de duiding van wettelijke beloningsnormen als onderdeel van publiekrechtelijke regulering in het civiel procesrecht en de hiervoor geldende rechterlijke toetsing.
Fenomenologie van het proces van bewijzen in strafzaken
Authors: de Jong Thomas Jacobus
Published: April 2019

Fenomenologie van het proces van bewijzen in strafzaken

In deze bijdrage staat de activiteit van bewijzen in strafzaken centraal. Betoogd wordt dat de vigerende rationalistische opvatting van strafrechtelijk bewijzen eraan voorbij gaat dat het bewijzen zich allereerst voltrekt op een vóór-reflectief niveau. Het primaire blikveld van de mens is namelijk niet het objectiverende kennen, zoals in de rationele bewijstheorieën wordt voorondersteld, maar de praktische relatie tot de wereld. In dit kader wordt eerst de filosofische achtergrond van de rationalistische bewijsopvatting in kaart gebracht, in het bijzonder de invloed van Aristoteles en Descartes. Vervolgens worden de daaruit voortkomende bevindingen aan de hand van ideeën en inzichten die zijn ontleend aan de existentiële fenomenologie kritisch gewaardeerd. Dit leidt tot de uiteenzetting van een hermeneutische opvatting van strafrechtelijk bewijzen.
Acetaminophen Serum Concentrations in Infants Treated Intravenously for Patent Ductus Arteriosus
Authors: McPherson Christopher , Luecke Caitlyn M. , Liviskie Caren J. , Zeller Brandy N. , Vesoulis Zachary A.
Published: April 2019

OBJECTIVE Although acetaminophen has emerged as a therapeutic option for treating hemodynamically significant patent ductus arteriosus (PDA) in preterm infants, limited data exist on pharmacodynamics. The objective of this research is to report serum acetaminophen concentrations at steady state in infants treated with intravenous acetaminophen for PDA and to examine associations with clinical outcomes.

METHODS This retrospective study evaluated all infants admitted during the study period who received intravenous acetaminophen for the treatment of PDA. Acetaminophen dosing was 15 mg/kg every 6 hours. A serum acetaminophen concentration was obtained 4 hours after the eighth dose. Associations between serum concentrations and efficacy, assessed by ductal constriction on echocardiogram, and safety, assessed by serum creatinine and hepatic transaminases, were explored using simple linear regression.

RESULTS A total of 36 infants were included, with a median birth weight of 720 g (IQR 585–895 g) and a median gestational age of 25 weeks (IQR 24–26 weeks). The median acetaminophen concentration in the cohort was 12.3 mg/L (IQR 6.7–16.5 mg/L; range, 1.1–29.0 mg/L). Serum acetaminophen concentrations did not correlate with infant demographics, hepatic transaminases during treatment, or duct size at treatment completion. We observed ductal closure across a wide range of serum acetaminophen concentrations.

CONCLUSIONS We did not identify an association between acetaminophen serum concentrations following intravenous therapy and ductal response or hepatic toxicity.
Graft-to-Recipient Weight Ratio Associated With Tacrolimus Metabolism Following Pediatric Living Donor Liver Transplantations
Authors: Shoji Kensuke , Miyairi Isao , Inoue Eisuke , Fukuda Akinari , Sakamoto Seisuke , Kasahara Mureo
Published: April 2019

OBJECTIVES Tacrolimus (TAC) is an important immunosuppressant in liver transplantation. Since TAC is mainly metabolized by the liver enzymes CYP3A4 and 5, liver function is crucial for its pharmacokinetics (PK). Liver function is dynamic after liver transplantation; hence the PK of TAC metabolism after pediatric liver transplantation is not well understood. We aimed to investigate the time-dependent changes in TAC metabolism and to find factors influencing TAC PK after pediatric liver transplantation.

METHODS We retrospectively reviewed the characteristics of the donors and recipients in pediatric living donor liver transplantation and used the TAC concentration-dose (CD) ratio as a surrogate marker of TAC metabolism.

RESULTS Included were 326 patients with a median age of 13 months. After the liver transplantation, the CD ratio gradually decreased, then plateaued around day 21 to 28. A linear regression analysis demonstrated that a lower graft-to-recipient weight ratio (GRWR) and higher prothrombin time–international normalized ratio (PT-INR) were independently associated with a higher CD ratio in the early period after liver transplantation. However, association between GRWR and TAC CD ratio disappeared around 6 to 12 months after a liver transplantation possibly owing to graft regeneration.

CONCLUSIONS Tacrolimus metabolism improved within the first month after liver transplantation, and the small graft size was associated with lower TAC metabolism in the early period after pediatric living donor liver transplantation.
Hypothermia in an Adolescent Due to Probable Drug-Drug Interaction Involving Clobazam
Authors: Parman Malinda G. , Holmes Amy P.
Published: April 2019

We report on a 16-year-old female who developed hypothermia as a result of a drug-drug interaction that produced supratherapeutic serum concentrations of clobazam. Although clobazam and its active metabolite (N-desmethylclobazam) are metabolized by cytochrome 2C19 (CYP2C19), literature suggests that clobazam-associated drug interactions involving this isoenzyme are not clinically relevant because of its wide therapeutic index. This report describes clobazam-associated hypothermia due to supratherapeutic serum concentrations of clobazam that resulted from the combination of 2 CYP2C19 inhibitors.
Cognitive Improvements in Children with Prader-Willi Syndrome Following Pitolisant Treatment—Patient Reports
Authors: Pullen Lara C. , Picone Maria , Tan Litjen , Johnston Charles , Stark Holger
Published: April 2019

While children with Prader-Willi Syndrome (PWS), a rare genetic disease with an incidence of 1:15,000, typically present with hypotonia and hyperphagia, their lives are made more difficult by an ever-present sleepiness as well as multiple neuro-cognitive dysfunctions, including cognitive defects. We describe a case series of 3 children who were treated with the histamine 3 receptor inverse agonist pitolisant. While this first-in-class inverse agonist is approved for another orphan disease (i.e., narcolepsy with or without cataplexy), we have observed that pediatric patients with PWS prescribed pitolisant demonstrate decreased daytime sleepiness and improved cognition, as evidenced by increased processing speed and improved mental clarity. Pitolisant may represent a novel therapeutic option that might relieve substantial PWS disease burden, including cognitive disability, excessive daytime sleepiness, and poor-quality nighttime sleep.
Overview and Preparation Guide for Academic Careers in Pediatric Pharmacy, Part 1: Career as a Pediatric Pharmacy Practice Faculty Member
Authors: Siu Anita , Blackmer Allison B. , Thornton Angela M. , Johnson Peter N. , Nichols Kristen R. , Hagemann Tracy M.
Published: April 2019

Pediatric clinical pharmacy is a growing and evolving field with an increasing number of pediatric clinical pharmacists in academia. In 2017, pediatric practice faculty members represented approximately 7.6% of all pharmacy practice faculty in the United States. The benefits of practicing in an academic environment are many, including, but not limited to, the ability to shape the future of pharmacy practice through the training of the next generation of pharmacists, contributing to science through research and scholarly activities for the care of pediatric patients, and positively impacting patient care for the most vulnerable of patients. Part one of this two-part series describes careers in academic pediatric pharmacy, as well as faculty roles and responsibilities, and provides information and advice related to the preparation and transition into careers in academic pediatric pharmacy.
Technology for Augmenting Type 1 Diabetes Mellitus Management
Authors: Condren Michelle , Sabet Samie , Chalmers Laura J. , Saley Taylor , Hopwood Jenna
Published: April 2019

Type 1 diabetes mellitus has witnessed significant progress in its management over the past several decades. This review highlights technologic advancements in type 1 diabetes management. Continuous glucose monitoring systems are now available at various functionality and cost levels, addressing diverse patient needs, including a recently US Food and Drug Administration (FDA)–approved implantable continuous glucose monitoring system (CGMS). Another dimension to these state-of-the-art technologies is CGMS and insulin pump integration. These integrations have allowed for CGMS-based adjustments to basal insulin delivery rates and suspension of insulin delivery when a low blood glucose event is predicted. This review also includes a brief discussion of upcoming technologies such as patch-based CGMS and insulin-glucagon dual-hormonal delivery.
Uporedna analiza upotrebe mobilnog bankarstva u Republici Srpskoj
Authors: Jokić Stevo
Published: April 2019

Mobilno bankarstvo je do sada pokrenulo uspavano tržište iučinilo bankarstvo efikasnijim i jednostavnijim. Nova tehnologijaje potpuno promenila način poslovanja i zamenila tradicionalnobankarstvo. Upotreba mobilnih telefona našla je svoju primenu iomogućila svakom klijentu mogućnost vođenja i održavanajasopstvenog računa. Sve ove pogodnosti dovele su do smanjenjatroškova usluge banke, što je uticalo na to da one ponude jeftinijeusluge na internetu. U svoj fokus banke su stavila samogpotrošača kako bi presonalizovale ponudu koja bi odgovaralasvakom klijentu posebno. Uslovi na tržištu dali su signal da imamogućnosti i za dalji razvoj i saradnju sa ostalim delatnostima.Nova era tehnologije dovela je do stvaranja digitalnih i mobilnihbanaka. U ovom radu opisano je trenutno bankarsko stanje uRepublici Srpskoj i delimično BIH, mogućnost za dalji napredakkao i budući trendovi razvoja mobilnog bankarstva.
Prestanak radnog odnosa i prava radnika
Authors: Janković Milorad , Ateljević Milutin
Published: April 2019

U ovom radu obrađeni su razlozi za prestanakugovora o radu radnika u slučajevima kada za prestanak postojikrivica radnika i u slučajevima kada ta krivica ne postoji, uznavođenje prava i instrumenata koje radnici mogu koristiti priprestanku radnog odnosa. Navedene su i neke od politika ipristupa koji se poslednjih godina afirmišu na evropskom tlu, uzukazivanje na prednosti i izazove koje ove politike moguproizvoditi.
Prednosti i mane korišćenja mobilnih aplikacija u učenju stranog jezika na primeru studentske populacije u Srbiji
Authors: Gajić Tijana , Maenza Neda
Published: April 2019

Savremeni uređaji sa mnoštvom raznovrsnihaplikacija su dramatično podigli interesovanje za konceptmobilnog, odnosno, m-učenja. Standardne karakteristikemobilnih telefona podrazumevaju pristup internetu, a samimtim, i neograničeni pristup edukativnom materijalu na svakommestu i u svakom trenutku. Sve ove odlike pospešujukomunikativne sposobnosti na stranom jeziku, podstičuinteresovanje za rešavanje zadataka koji su predstavljeni nakreativan i inovativan način, blizak mlađoj populaciji, ujednodoprinoseći razvoju svih jezičkih veština. U radu je predstavljenoistraživanje sprovedeno sa ciljem ispitivanja stavova studenata odobrim i lošim stranama korišćenja mobilnih aplikacija zaučenje jezika. Rad takođe nudi opis metodologije istraživanja,načina obrade podataka i interpretacije dobijenih rezultata. Nakraju, nakon sumiranja rezultata, date su preporuke kako zaeventualna dalja istraživanja tako i za što uspešnije iproduktivnije korišćenje mobilnih aplikacija u nastavi stranihjezika.
The use of audio and video recordings in English language teaching
Authors: Nikolić Milena
Published: April 2019

By introducing and depicting the assignments setbefore the third-year-students of the Faculty of Philology atSinergija University in Bijeljina enrolled in the course ofDidactics, we tried to identify the innovative ways of using audioand video recordings inside and outside the classroom and touchupon their numerous positive effects on foreign languageteaching and learning. The students’ smartphones were used inperforming the tasks. Task 1 establishes a link between literaryproficiency and the use of online newspapers. Task 2 involvesstudents’ moving to a new location, linking learning a foreignlanguage to the students’ real world experience and learningabout nonverbal communication. Task 3 presents the way inwhich an inaccessible literary text is likely to become amusingand accessible by means of digital devices.
Next-generation sequencing of BRCA1 and BRCA2 genes for rapid detection of germline mutations in hereditary breast/ovarian cancer
Authors: Nicolussi Arianna , Belardinilli Francesca , Mahdavian Yasaman , Colicchia Valeria , D’Inzeo Sonia , Petroni Marialaura , Zani Massimo , Ferraro Sergio , Valentini Virginia , Ottini Laura , Giannini Giuseppe , Capalbo Carlo , Coppa Anna
Published: April 2019

Background

Conventional methods used to identify BRCA1 and BRCA2 germline mutations in hereditary cancers, such as Sanger sequencing/multiplex ligation-dependent probe amplification (MLPA), are time-consuming and expensive, due to the large size of the genes. The recent introduction of next-generation sequencing (NGS) benchtop platforms offered a powerful alternative for mutation detection, dramatically improving the speed and the efficiency of DNA testing. Here we tested the performance of the Ion Torrent PGM platform with the Ion AmpliSeq BRCA1 and BRCA2 Panel in our clinical routine of breast/ovarian hereditary cancer syndrome assessment.

Methods

We first tested the NGS approach in a cohort of 11 patients (training set) who had previously undergone genetic diagnosis in our laboratory by conventional methods. Then, we applied the optimized pipeline to the consecutive cohort of 136 uncharacterized probands (validation set).

Results

By minimal adjustments in the analytical pipeline of Torrent Suite Software we obtained a 100% concordance with Sanger results regarding the identification of single nucleotide alterations, insertions, and deletions with the exception of three large genomic rearrangements (LGRs) contained in the training set. The optimized pipeline applied to the validation set (VS), identified pathogenic and polymorphic variants, including a novel BRCA2 pathogenic variant at exon 3, 100% of which were confirmed by Sanger in their correct zygosity status. To identify LGRs, all negative samples of the VS were subjected to MLPA analysis.

Discussion

Our experience strongly supports that the Ion Torrent PGM technology in BRCA1 and BRCA2 germline variant identification, combined with MLPA analysis, is highly sensitive, easy to use, faster, and cheaper than traditional (Sanger sequencing/MLPA) approaches.
MicroRNA profiling of mouse liver in response to DENV-1 infection by deep sequencing
Authors: Pong Lian Yih , Parkkinen Sinikka , Dhanoa Amreeta , Gan Han Ming , Wickremesinghe Indeevari Abisheka Chiharu , Syed Hassan Sharifah
Published: April 2019

Background

Dengue caused by dengue virus (DENV) serotypes −1 to −4 is the most important mosquito-borne viral disease in the tropical and sub-tropical countries worldwide. Yet many of the pathophysiological mechanisms of host responses during DENV infection remain largely unknown and incompletely understood.

Methods

Using a mouse model, the miRNA expressions in liver during DENV-1 infection was investigated using high throughput miRNA sequencing. The differential expressions of miRNAs were then validated by qPCR, followed by target genes prediction. The identified miRNA targets were subjected to gene ontology (GO) annotation and pathway enrichment analysis to elucidate the potential biological pathways and molecular mechanisms associated with DENV-1 infection.

Results

A total of 224 and 372 miRNAs out of 433 known mouse miRNAs were detected in the livers of DENV-1-infected and uninfected mice, respectively; of these, 207 miRNAs were present in both libraries. The miR-148a-3p and miR-122-5p were the two most abundant miRNAs in both groups. Thirty-one miRNAs were found to have at least 2-fold change in upregulation or downregulation, in which seven miRNAs were upregulated and 24 miRNAs were downregulated in the DENV-1-infected mouse livers. The miR-1a-3p was found to be the most downregulated miRNA in the DENV-1-infected mouse livers, with a significant fold change of 0.10. To validate the miRNA sequencing result, the expression pattern of 12 miRNAs, which were highly differentially expressed or most abundant, were assessed by qPCR and nine of them correlated positively with the one observed in deep sequencing.In silicofunctional analysis revealed that the adaptive immune responses involving TGF-beta, MAPK, PI3K-Akt, Rap1, Wnt and Ras signalling pathways were modulated collectively by 23 highly differentially expressed miRNAs during DENV-1 infection.

Conclusion

This study provides the first insight into the global miRNA expressions of mouse livers in response to DENV-1 infectionin vivoand the possible roles of miRNAs in modulating the adaptive immune responses during DENV-1 infection.
Integrated analysis of two-lncRNA signature as a potential prognostic biomarker in cervical cancer: a study based on public database
Authors: Wu Wenjuan , Sui Jing , Liu Tong , Yang Sheng , Xu Siyi , Zhang Man , Huang Shaoping , Yin Lihong , Pu Yuepu , Liang Geyu
Published: April 2019

Background

Cervical cancer (CC) is a common gynecological malignancy in women worldwide. Evidence suggests that long non-coding RNAs (lncRNAs) can be used as biomarkers in patients with CC. However, prognostic biomarkers for CC are still lacking. The aim of our study was to find lncRNA biomarkers which are able to predict prognosis in CC based on the data from The Cancer Genome Atlas (TCGA).

Methods

The patients were divided into three groups according to FIGO stage. Differentially expressed lncRNAs were identified in CC tissue compared to adjacent normal tissues based on a fold change >2 and <0.5 at P < 0.05 for up- and downregulated lncRNA, respectively. The relationship between survival outcome and lncRNA expression was assessed with univariate and multivariate Cox proportional hazards regression analysis. We constructed a risk score as a method to evaluate prognosis. We used receiver operating characteristic (ROC) curve and the area under curve (AUC) analyses to assess the diagnostic value of a two-lncRNA signature. We detected the expression levels of the two lncRNAs in 31 pairs of newly diagnosed CC specimens and paired adjacent non-cancerous tissue specimens, and also in CC cell lines. Finally, the results were statistically compared using t-tests.

Results

In total, 289 RNA sequencing profiles and accompanying clinical data were obtained. We identified 49 differentially expressed lncRNAs, of which two related to overall survival (OS) in CC patients. These two lncRNAs (ILF3-AS1 and RASA4CP) were found together as a single prognostic signature. Meanwhile, the prognosis of patients with low-risk CC was better and positively correlated with OS (P < 0.001). Further analysis showed that the combined two-lncRNA expression signature could be used as an independent biomarker to evaluate the prognosis in CC. qRT-PCR results were consistent with TCGA, confirming downregulated expression of both lncRNAs. Furthermore, upon ROC curve analysis, the AUC of the combined lncRNAs was greater than that of the single lncRNAs alone (0.723 vs 0.704 and 0.685), respectively; P < 0.05.

Conclusions

Our study showed that the two-lncRNA signature of ILF3-AS1 and RASA4CP can be used as an independent biomarker for the prognosis of CC, based on bioinformatic analysis.
General journal policies and guidelines for publication in Ciencias Marinas
Authors: De Jesus Melba
Published: April 2019

The following document is an enhanced, extended version of our previously known Instructions for Authors. It was elaborated to give you more detailed information about our journal foundations and our basic requirements for publication in Ciencias Marinas. This extended version first addresses general questions about our journal so you know where you are publishing. It then describes our journal policies, and it finally elaborates on submission and manuscript preparation guidelines. Modifications to this document will undoubtedly be made in the future as we undertake arising issues, but for now we hope it serves your needs with regard to Ciencias Marinas, or CM as we will refer to it from now on except in headings.
Factors Affecting Farm Mechanization – A Case Study in Assam, India
Authors: Barman Sinki , Deka Nivedita , Deka Pallavi
Published: April 2019

The study was conducted in Central Brahmaputra Valley and Upper Brahmaputra Valley Zone of Assam in India. Primary data of 240 sample farms by personal interview schedule method and Logit Regression Analysis was used for examining the factors affecting farm mechanization adoption. All data collected from sample farms pertains to the year 2014-15.With the help of logit regression different factors affecting the mechanization adoption was examined in the sample households where seven explanatory variable to explain mechanization adoption viz., age of the head of household i.e. AGE, education level of the household i.e. EDU, size of land holdings i.e. LHD, access to irrigation i.e. IRA, access to extension agents i. e.  EXT, area under high yielding varieties i.e. HYA  and the access to institutional credit i.e. BLN were included and the negative value of the coefficient of AGE showed that the younger generation of farmers favoured the mechanization of farm much more compared to the old block. The coefficient of EDU (4.325) was positive and highly significant level confirming that the adoption of farm mechanization was more prevalent among the farms having relatively literate in the study area. It was found from the above analysis that there were different factors which affect the farm mechanization.  Linkage of extension functionaries with the grassroots level by creating awareness about the use of farm machineries amongst the farmers.
Plug Cell Volume, Growing Media Quality and Benzyl Aminopurine (BAP) Spray Effects for Nursery Growth of Impatiens walleriana
Authors: Lojo J. De , Gandolfo E. , Giardina E. , Boschi C. , Benedetto A. Di
Published: April 2019

Plant propagators must take two technological critical decisions: the plug-cell size and the growing medium, both of which have been mentioned as abiotic stress sources for bedding pot plants. However, only a few recent reports on bedding pot plants have simultaneously included limiting and non-limiting plug cell volumes and growing medium during nursery. The aim of this work was to assess the nursery performance of Impatiens walleriana seedlings grown in four plug cell volumes and four growing media with significant differences in both physical and chemical properties. Plants were sprayed or not with an early and single benzyl aminopurine (BAP) dose, aiming to understand how they interact on determining biomass accumulation at the pot transplant stage. The hypothesis tested was that, both plug cell volume and growing medium, must be seen as additive abiotic stress sources, which can be partially overridden by exogenous cytokinin supply. The main result was that, in I. walleriana seedlings, the abiotic stress imposed by the growing medium quality during nursery had a higher effect on biomass accumulation (on both fresh and dry base), leaf area expansion and photo assimilates partitioning than plug cell volume and constitute an interactive process associated with cytokinin synthesis. From a grower´s point of view, one expensive option to avoid root restriction is to use high quality growing media and increase the plug cell volume. In contrast, a single 100 mg L-1 BAP spray can partially override the root restriction symptoms related to abiotic stresses. The novelty of this work is related to the fact that growing media quality would be a more limited factor than plug cell volume for I. walleriana seedlings during nursery.
Infrastructure for Agricultural & Rural Development in Sub Saharan Africa: Need for a Comprehensive Program and Adequate Investment
Authors: OnyekaEzealaji Nwauwa Linus
Published: April 2019

This study unanimously confirms that rural infrastructure is a sine qua non for significantly improving the quality of human life and phenomenally accelerating the process of agricultural development in Africa. Infrastructure projects, however, involve huge initial capital investments, long gestation periods, high incremental capital output ratio, high risk, and low rate of returns on investments. Rural infrastructure has direct and strong relationship with farmers’ access to institutional finance and markets, and increasing crop yields, thereby promoting agricultural growth. Agricultural infrastructure has the potential to transform the existing traditional agriculture or subsistence farming into a most modern, commercial and dynamic farming system in Sub Saharan Africa. Increase in investment of agricultural infrastructure leads to increase in output and employment, a full investment formulation that meets the needs of domestic or external (multilateral and bilateral) funding sources will have to be carried out. Overall, a flexible, participatory approach will be needed, with full national and local involvement and commitment, while international partners, including Food and Agricultural Organization (FAO), give initial assistance to New partnership for Africa’s Development (NEPAD) in this process. The paper therefore recommends that technical and financial assistance will be required to help build capacity in African countries to face the challenges and take full advantage of the opportunities flowing from the multilateral trading systems.
A Case of Cholangiolocellular Carcinoma Preoperatively Diagnosed With Typical Imaging Findings
Authors: Mochizuki Yoshihiro , Iimuro Yuji , Suzuki Osamu , Nagashima Yoji
Published: April 2019

Introduction:

Cholangiolocellular carcinoma (CoCC) is a rare primary liver neoplasm. A recent integrative genomic analysis has revealed that CoCC represents a distinct biliary-derived molecular entity. Several cases of CoCC have been reported so far, but accurate preoperative diagnosis was difficult in most cases.

Case presentation:

We report a case of 70-year-old woman with CoCC. Preoperative imaging findings revealed several typical signs of CoCC (i.e., thick early ring enhancement in the peripheral area of the tumor and its prolongation, vessel penetration through the tumor, no dilatation of the peripheral bile ducts, and dot-/band-like internal enhancement or a target appearance on contrast-enhanced magnetic resonance imaging). We strongly suspected CoCC from these preoperative imaging findings of the tumor and performed extended left hepatectomy. Pathologic diagnosis was CoCC, and the histologic findings such as peripheral highly cellular areas, central abundant hyalinized/edematous fibrotic stroma, and retained Glisson's sheath structures in the tumor, corresponded closely to each preoperative imaging finding. Immunohistochemical study revealed the tumor cells were positive for cytokeratin 7 and epithelial membrane antigen. The postoperative course was uneventful, and the patient is alive without recurrence for 15 months. The prognosis of CoCC is known to be better than that of cholangiocellular carcinoma, indicating the importance of preoperative differential diagnosis of these tumors.

Conclusion:

Even though preoperative diagnosis of CoCC is difficult because of its rarity, cautious investigation of preoperative typical imaging findings can possibly lead to accurate diagnosis of CoCC.
EXPLORING THE RELATIONSHIP BETWEEN PROCEDURAL JUSTICE AND INNOVATIVE WORK BEHAVIOR IN HOSPITALITY INDUSTRY
Authors: Noerchoidah Noerchoidah , Harjanti Dhyah
Published: April 2019

The purpose of this study is to examine the relationship between procedural justice, know­ledge sharing and innovative work behavior. The research model and hypothesis were developed from related literature. The data was collected through survey method on 297 supervisors of four and five star hotels in East Java, Indonesia. The obtained data from the questionnaire were tested with Partial Least Square (PLS) to investigate the research model. The results confirm that pro­cedural justice has positive impact on knowledge sharing. While the effect of knowledge sharing on innovative work behavior is another finding of this research. The implications of this study are discussed in the conclusions section of the study
PENGARUH DIGITAL MARKETINGTERHADAP ORGANIZATIONAL PERFORMANCE DENGANINTELLECTUAL CAPITAL DAN PERCEIVED QUALITYSEBAGAI VARIABEL INTERVENING PADA INDUSTRI HOTEL BINTANG TIGA DI JAWA TIMUR
Authors: Prabowo Willy Andrian
Published: April 2019

The influence of Digital Marketing on Organizational Performance with Intellectual Capital and Perceived Quality as an intervening variables in Three Star Hospitality Industry in East Java.The growth of tourism in Indonesia is growing abundantly. People’s desire for vacation is developing through the years. This enables intense competiton in the Hospitality Industry in East Java.This research aims to analyze the influece of Digital Marketing (Interactive, Incentive Programs, Site design, Cost), to Organizational Performance in Hotel Industry in East Java with Intellectual Capital (Human Capital, Customer Capital, Structural Capital) and Perceived Quality (Tangibles, Reliability, Responsiveness, Assurance, Empathy), as an intervening variables.This research was conducted by distributing questionnaires to 120 respondents internal employees of Three Star Hotel in East Java. Analytical technique used in technical quantitative analysis with path analysis method
ANALISA PENGARUH SERVICE QUALITY TERHADAP BEHAVIORAL INTENTIONS DENGAN PERCEIVED VALUE DAN CUSTOMER SATISFACTION SEBAGAI VARIABEL INTERVENING PADA PENDIDIKAN TINGGI
Authors: Tandijaya Trixie Nova Bella
Published: April 2019

The importance of maintaining the quality of education of a country, making education in Indonesia also continues to make improvements both from the Elementary School level to the University level. At the University level alone, there is increasing competition between the State and Private Universities to produce more and more qualified graduates. So many universities are starting to think of strategies to win the competition. One of them by maintaining the Service Quality they provide. This study aims to determine the effect of Service Quality on Behavioral Intention or the tendency to behave, with perceived value and customer satisfaction as intervening variable high education students 2012-2016. The results of this study indicate that service quality has a positive and significant influence on perceived value and behavioral intentions, perceived value positively and significantly influence customer satisfaction and behavioral intentions, and customer satisfaction positively and significantly influence behavioral intentions. The results of this study also show that perceived value and customer satisfaction as intervening variables do not successfully mediate the relationship between service quality and behavioral intentions
PENGARUH RETAIL SERVICE QUALITY TERHADAP MINAT BERKUNJUNG ULANG MALL Di SURABAYA MELALUI PERCEIVED QUALITY DAN CUSTOMER SATISFACTION SEBAGAI VARIABEL INTERVENING
Authors: Japarianto Edwin
Published: April 2019

Perkembangan mall berdampak pada semakin meningkatnya pengunjung mall setiap tahun dan juga berdampak pada semakin tingginya persaingan dalam dunia tersebut khususnya di daerah Surabaya. Salah satu cara untuk dapat terus bersaing dan menigkatkan minat berkunjung ulang pengunjung ialah meningkatkan kualitas layanan. Meningkatkan kualitas layanan dapat membangun persepsi pelanggan terhadap mall tersebut yang nantinya akan berdampak pada kepuasan pengunjung serta akan meningkatkan minat pengunjung dalam melakukan kunjungan kembali. Penelitian ini bertujuan untuk menganalisa pengaruh Retail Service Quality terhadap Minat Berkunjung Ulang yang di-intervening  oleh Perceived Quality dan Customer Satisfaction pada mall. Penelitian ini dilakukan dengan menyebarkan angket / kuesioner kepada 100 orang responden yang telah berkunjung ke mall. Metode penelitian yang digunakan merupakan metode analisa kuantitatif dengan menggunakan Smart PLS.Hasil dari penelitian menunjukkan Retail Service Quality berpengaruh signifikan terhadap Minat Berkunjung Ulang dan variabel Perceived Quality dan Customer Satisfaction mengintervening hubungan Retail Service Quality dan Minat Berkunjung Ulang.
Occupational exposure to inhaled nanoparticles: Are young workers being left in the dust?
Authors: Graczyk Halshka , Riediker Michael
Published: April 2019

Objectives

Occupational exposure to inhaled nanoparticles (NPs) represents a significant concern for worker health. Adolescent workers may face unique risks for exposure and resulting health effects when compared with adult workers.

Methods

This manuscript discusses key differences in risks for occupational exposures to inhaled NPs and resulting health effects between young workers and adult workers via an examination of both physiological and occupational setting factors.

Results

Previous studies document how adolescents often face distinct and unique exposure scenarios to occupational hazards when compared to adults. Moreover, they also face different and unpredictable health effects because biological functions such as detoxification pathways and neurological mechanisms are still developing well into late adolescence. Early exposure also increases the chances of developing long-latency disease earlier in life.

Taken together, adolescents’ rapid growth and development encompasses highly dynamic and complex processes. An aggravating factor is that these processes do not necessarily fall in line with legal classifications of adulthood, nor with occupational exposure limits created for adult workers.

Conclusions

The differences in exposures and health consequences from NPs on young workers are insufficiently understood. Research is needed to better understand what adolescent-specific mitigation strategies may be most suitable to address these risk factors.