bluuebunny/crossref_metadata_2025 huggingface dataset:
LncRNAs as Therapeutic Targets and Potential Biomarkers for Lipid-Related Diseases
Authors: Huang Shi-Feng , Peng Xiao-Fei , Jiang Lianggui , Hu Ching Yuan , Ye Wen-Chu
Published: August 2021

Lipid metabolism is an essential biological process involved in nutrient adjustment, hormone regulation, and lipid homeostasis. An irregular lifestyle and long-term nutrient overload can cause lipid-related diseases, including atherosclerosis, myocardial infarction (MI), obesity, and fatty liver diseases. Thus, novel tools for efficient diagnosis and treatment of dysfunctional lipid metabolism are urgently required. Furthermore, it is known that lncRNAs based regulation like sponging microRNAs (miRNAs) or serving as a reservoir for microRNAs play an essential role in the progression of lipid-related diseases. Accordingly, a better understanding of the regulatory roles of lncRNAs in lipid-related diseases would provide the basis for identifying potential biomarkers and therapeutic targets for lipid-related diseases. This review highlighted the latest advances on the potential biomarkers of lncRNAs in lipid-related diseases and summarised current knowledge on dysregulated lncRNAs and their potential molecular mechanisms. We have also provided novel insights into the underlying mechanisms of lncRNAs which might serve as potential biomarkers and therapeutic targets for lipid-related diseases. The information presented here may be useful for designing future studies and advancing investigations of lncRNAs as biomarkers for diagnosis, prognosis, and therapy of lipid-related diseases.
Enhanced Vasoconstriction Mediated by α1-Adrenergic Mechanisms in Small Femoral Arteries in Newborn Llama and Sheep Gestated at Low and High Altitudes
Authors: Moraga Fernando A. , Reyes Roberto V. , Ebensperger Germán , López Vasthi , Llanos Aníbal J.
Published: August 2021

The authors previously demonstrated that newborn llama (NBLL) express high levels of α1 adrenergic receptors, which provide a potent vasoconstriction response when compared with newborn sheep (NBSH) gestated at sea level. However, data regarding the impact of chronic gestational hypobaric hypoxia on α-adrenergic vasoconstriction in the neonatal life has not been studied. We evaluated if gestation under chronic hypobaric hypoxia modifies α1-adrenergic vasoconstrictor function in NBLL and NBSH. We compared the vasoconstrictor response induced by potassium and α-adrenergic stimuli in isolated small femoral arteries of NBLL and NBSH gestated at high altitude (HA; 3,600 m) or low altitude (LA; 580 m). The maximal contraction (RMAX) and potency (EC50) to potassium, noradrenaline (NA), and phenylephrine (PHE) were larger in HA-NBLL than LA-NBLL. RMAX to potassium, NA, and PHE were lower in HA-NBSH when compared with LA-NBSH and potency results were similar. Competitive blockade with prazosin showed that RNLL LA/HA have a similar pA2. In contrast, NBSH had increased pA2 values in HA when compared with LA. Finally, small femoral arteries denudated or treated with LNAME in LA and HA lacked NO or endothelium participation in response to PHE stimulation. In contrast, NBSH displayed that denudation or blockade with LNAME support NO or endothelium participation in response to PHE activation. In conclusion, HA chronic hypoxia enhances α1 adrenergic receptor activity in small femoral arteries in NBLL to a higher degree than NBSH, implying a higher vasoconstriction function.
Extracellular Matrix Protein Ratios in the Human Heart and Vessels: How to Distinguish Pathological From Physiological Changes?
Authors: Wittig Corey , Szulcek Robert
Published: August 2021

Cardiovascular pathology is often accompanied by changes in relative content and/or ratios of structural extracellular matrix (ECM) proteins within the heart and elastic vessels. Three of these proteins, collagen-I, collagen-III, and elastin, make up the bulk of the ECM proteins in these tissues, forming a microenvironment that strongly dictates the tissue biomechanical properties and effectiveness of cardiac and vascular function. In this review, we aim to elucidate how the ratios of collagen-I to collagen-III and elastin to collagen are altered in cardiovascular diseases and the aged individuum. We elaborate on these major cardiovascular ECM proteins in terms of structure, tissue localization, turnover, and physiological function and address how their ratios change in aging, dilated cardiomyopathy, coronary artery disease with myocardial infarction, atrial fibrillation, aortic aneurysms, atherosclerosis, and hypertension. To the end of guiding in vitro modeling approaches, we focus our review on the human heart and aorta, discuss limitations in ECM protein quantification methodology, examine comparability between studies, and highlight potential in vitro applications. In summary, we found collagen-I relative concentration to increase or stay the same in cardiovascular disease, resulting in a tendency for increased collagen-I/collagen-III and decreased elastin/collagen ratios. These ratios were found to fall on a continuous scale with ranges defining distinct pathological states as well as a significant difference between the human heart and aortic ECM protein ratios.
Muscle in Variable Gravity: “I Do Not Know Where I Am, But I Know What to Do”
Authors: Monti Elena , Waldvogel Janice , Ritzmann Ramona , Freyler Kathrin , Albracht Kirsten , Helm Michael , De Cesare Niccolò , Pavan Piero , Reggiani Carlo , Gollhofer Albert , Narici Marco Vincenzo
Published: August 2021

Purpose: Fascicle and sarcomere lengths are important predictors of muscle mechanical performance. However, their regulation during stretch-shortening cycle (SSC) activities in usual and challenging conditions is poorly understood. In this study, we aimed to investigate muscle fascicle and sarcomere behavior during drop jumps (a common SSC activity) in conditions of variable gravity.

Methods: Fifteen volunteers performed repeated drop jumps in 1 g, hypo-gravity (0 to 1 g), and hyper-gravity (1 to 2 g) during a parabolic flight. Gastrocnemius medialis (GM) electromyographic activity and fascicle length (Lf) were measured at drop-off, ground contact (GC), minimum ankle joint angle (MAJ), and push-off. GM sarcomere number was estimated by dividing Lf, measured by ultrasound at rest, by published data on GM sarcomere length, and measured in vivo at the same joint angle. Changes in sarcomere length were estimated by dividing GM Lf in each jump phase by sarcomere number calculated individually. The sarcomere force-generating capacity in each jump phase was estimated from the sarcomere length-tension relationship previously reported in the literature.

Results: The results showed that, regardless of the gravity level, GM sarcomeres operated in the ascending portion of their length-tension relationship in all the jump phases. Interestingly, although in hypo-gravity and hyper-gravity during the braking phase (GC-MAJ) GM fascicles and sarcomeres experienced a stretch (as opposed to the quasi-isometric behavior in 1 g), at MAJ they reached similar lengths as in 1 g, allowing sarcomeres to develop about the 70% of their maximum force.

Conclusion: The observed fascicle behavior during drop jumping seems useful for anchoring the tendon, enabling storage of elastic energy and its release in the subsequent push-off phase for effectively re-bouncing in all gravity levels, suggesting that an innate neuromuscular wisdom enables to perform SSC movements also in challenging conditions.
Evaluation of Light-Dependent Photosynthetic Reactions in Reynoutria japonica Houtt. Leaves Grown at Different Light Conditions
Authors: Mlinarić Selma , Begović Lidija , Tripić Neven , Piškor Antonija , Cesar Vera
Published: August 2021

The Japanese knotweed (Reynoutria japonica Houtt.) is considered as one of the most aggressive and highly successful invasive plants with a negative impact on invaded habitats. Its uncontrolled expansion became a significant threat to the native species throughout Europe. Due to its extensive rhizome system, rapid growth, and allelopathic activity, it usually forms monocultures that negatively affect the nearby vegetation. The efficient regulation of partitioning and utilization of energy in photosynthesis enables invasive plants to adapt rapidly a variety of environmental conditions. Therefore, we aimed to determine the influence of light conditions on photosynthetic reactions in the Japanese knotweed. Plants were grown under two different light regimes, namely, constant low light (CLL, 40 μmol/m2/s) and fluctuating light (FL, 0–1,250 μmol/m2/s). To evaluate the photosynthetic performance, the direct and modulated chlorophyll a fluorescence was measured. Plants grown at a CLL served as control. The photosynthetic measurements revealed better photosystem II (PSII) stability and functional oxygen-evolving center of plants grown in FL. They also exhibited more efficient conversion of excitation energy to electron transport and an efficient electron transport beyond the primary electron acceptor QA, all the way to PSI. The enhanced photochemical activity of PSI suggested the formation of a successful adaptive mechanism by regulating the distribution of excitation energy between PSII and PSI to minimize photooxidative damage. A faster oxidation at the PSI side most probably resulted in the generation of the cyclic electron flow around PSI. Besides, the short-term exposure of FL-grown knotweeds to high light intensity increased the yield induced by downregulatory processes, suggesting that the generation of the cyclic electron flow protected PSI from photoinhibition.
Impacts of Nitrogen Deficiency on Wheat (Triticum aestivum L.) Grain During the Medium Filling Stage: Transcriptomic and Metabolomic Comparisons
Authors: Wang Yanjie , Wang Demei , Tao Zhiqiang , Yang Yushuang , Gao Zhenxian , Zhao Guangcai , Chang Xuhong
Published: August 2021

Nitrogen (N) supplementation is essential to the yield and quality of bread wheat (Triticum aestivum L.). The impact of N-deficiency on wheat at the seedling stage has been previously reported, but the impact of distinct N regimes applied at the seedling stage with continuous application on filling and maturing wheat grains is lesser known, despite the filling stage being critical for final grain yield and flour quality. Here, we compared phenotype characteristics such as grain yield, grain protein and sugar quality, plant growth, leaf photosynthesis of wheat under N-deficient and N-sufficient conditions imposed prior to sowing (120 kg/hm2) and in the jointing stage (120 kg/hm2), and then evaluated the effects of this continued stress through RNA-seq and GC-MS metabolomics profiling of grain at the mid-filling stage. The results showed that except for an increase in grain size and weight, and in the content of total sugar, starch, and fiber in bran fraction and white flour, the other metrics were all decreased under N-deficiency conditions. A total of 761 differentially expressed genes (DEGs) and 77 differentially accumulated metabolites (DAMs) were identified. Under N-deficiency, 51 down-regulated DEGs were involved in the process of impeding chlorophyll synthesis, chloroplast development, light harvesting, and electron transfer functions of photosystem, which resulted in the SPAD and Pn value decreased by 32 and 15.2% compared with N-sufficiency, inhibited photosynthesis. Twenty-four DEGs implicated the inhibition of amino acids synthesis and protein transport, in agreement with a 17–42% reduction in ornithine, cysteine, aspartate, and tyrosine from metabolome, and an 18.6% reduction in grain protein content. However, 14 DEGs were implicated in promoting sugar accumulation in the cell wall and another six DEGs also enhanced cell wall synthesis, which significantly increased fiber content in the endosperm and likely contributed to increasing the thousands-grain weight (TGW). Moreover, RNA-seq profiling suggested that wheat grain can improve the capacity of DNA repair, iron uptake, disease and abiotic stress resistance, and oxidative stress scavenging through increasing the content levels of anthocyanin, flavonoid, GABA, galactose, and glucose under N-deficiency condition. This study identified candidate genes and metabolites related to low N adaption and tolerance that may provide new insights into a comprehensive understanding of the genotype-specific differences in performance under N-deficiency conditions.
Flavonoid Levels and Antioxidant Capacity of Mulberry Leaves: Effects of Growth Period and Drying Methods
Authors: Hu Lei , Wang Cheng , Guo Xiang , Chen Dekui , Zhou Wei , Chen Xiaoyang , Zhang Qing
Published: August 2021

In recent years, various mulberry leaf dishes have gradually gained in popularity. The harvesting period of mulberry leaves and the preparation of dried samples are critical for the retention of flavonoid content and activity. In this study, changes in flavonoid levels in mulberry leaves during their growth period (3–6 months), and the effects of four different drying methods [sun drying (SD), air drying (AD), oven drying (OD), and freeze drying (FD)] on flavonoid accumulation and antioxidant capacity were determined. The results showed that mulberry leaves grown for 6 months had higher levels of flavonoids, and different drying methods could significantly affect the flavonoid levels and antioxidant capacity of the leaves. Air drying and FD were the best methods in terms of retaining the antioxidant activity of flavonoids, although AD had lower operating costs than FD in the production process. Therefore, to obtain a high flavonoid content and maximum antioxidant activity, AD is recommended for mulberry leaves.
The GA 20-Oxidase Encoding Gene MSD1 Controls the Main Stem Elongation in Medicago truncatula
Authors: Li Wanying , Ma Qingxia , Yin Pengcheng , Wen Jiangqi , Pei Yanxi , Niu Lifang , Lin Hao
Published: August 2021

Plant height is an important agronomic trait that is closely related to biomass yield and crop production. Despite legumes comprise one of the largest monophyletic families that are second only to grasses in terms of economic and nutritional values, due to an ancient genome duplication event, most legume plants have complex genomes, thus the molecular mechanisms that determine plant height are less known in legumes. Here, we report the identification and characterization of MAIN STEM DWARF1 (MSD1), which is required for the plant height in the model legume Medicago truncatula. Loss of function of MSD1 leads to severely reduced main stem height but normal lateral branch elongation in M. truncatula. Histological analysis revealed that the msd1-1 main stem has shorter internodes with reduced cell size and number compared with the wild type, indicating that MSD1 affects cell elongation and cell proliferation. MSD1 encodes a putative GA 20-oxidase that is expressed at significantly higher levels in the main shoot apex than in the lateral shoot apices, suggesting that MSD1 expression is associated with its effect on the main stem elongation. UPLC-MS/MS analysis showed that GA9 and GA4, two identified products of the GA 20-oxidase, were severely reduced in msd1-1, and the dwarf phenotype of msd1-1 could be rescued by supplementation with gibberellic acid GA3, confirming that MSD1 functions as a biologically active GA 20-oxidase. Moreover, we found that disruption of either MtGA20ox7 or MtGA20ox8, homologs of MSD1, has little effects on the elongation of the main stem, while the msd1-1 mtga20ox7-1 mtga20ox8 triple mutants exhibits a severe short main shoot and lateral branches, as well as reduced leaf size, suggesting that MSD1 and its homologs MtGA20ox7 and MtGA20ox8, redundantly regulate M. truncatula shoot elongation and leaf development. Taken together, our findings demonstrate the molecular mechanism of MSD1-mediated regulation of main stem elongation in M. truncatula and provide insights into understanding the functional diversity of GA 20-oxidases in optimizing plant architecture in legumes.
14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida
Authors: Lin Xiaohui , Huang Shina , Huang Gan , Chen Yanbo , Wang Xiaojing , Wang Yaqin
Published: August 2021

14-3-3 proteins play a major role in the regulation of primary metabolism, protein transport, ion channel activity, signal transduction and biotic/abiotic stress responses. However, their involvement in petal growth and development is largely unknown. Here, we identified and characterized the expression patterns of seven genes of the 14-3-3 family in gerbera. While none of the genes showed any tissue or developmental specificity of spatiotemporal expression, all seven predicted proteins have the nine α-helices typical of 14-3-3 proteins. Following treatment with brassinolide, an endogenous brassinosteroid, the Gh14-3-3 genes displayed various response patterns; for example, Gh14-3-3b and Gh14-3-3f reached their highest expression level at early (2 h) and late (24 h) timepoints, respectively. Further study revealed that overexpression of Gh14-3-3b or Gh14-3-3f promoted cell elongation, leading to an increase in ray petal length. By contrast, silencing of Gh14-3-3b or Gh14-3-3f inhibited petal elongation, which was eliminated partly by brassinolide. Correspondingly, the expression of petal elongation-related and brassinosteroid signaling-related genes was modified in transgenic petals. Taken together, our research suggests that Gh14-3-3b and Gh14-3-3f are positive regulators of brassinosteroid-induced ray petal elongation and thus provides novel insights into the molecular mechanism of petal growth and development.
Spiritual Inspiration of Village Cadres and Inclusive Innovation of Bricolage in Rural Autonomy in China
Authors: Zhao Qing , Yu Mei , Liu Liangcan , Li Binghan , Feng Li
Published: August 2021

In the rural autonomous context of China, inclusive innovation of bricolage is a widespread phenomenon. However, the existing research rarely considers villagers as the main actors of innovation. In a research survey of 23 provinces in China, we studied the internal mechanism of inclusive innovation of bricolage. We found that the spiritual inspiration of the village cadres is positively related to inclusive innovation of bricolage behavior of villagers. Moreover, the results revealed that affective commitment to the village of the villagers plays a mediating role in the relationship between the spiritual inspiration of the village cadres and inclusive innovation of bricolage behavior of villagers. In addition, our findings reveal that resilience of villagers plays a moderating role in the relationship between the spiritual inspiration of the village cadres and inclusive innovation of bricolage behavior of villagers.
Biopsychosocial Functions of Human Walking and Adherence to Behaviourally Demanding Belief Systems: A Narrative Review
Authors: O’Mara Shane
Published: August 2021

Human walking is a socially embedded and shaped biological adaptation: it frees our hands, makes our minds mobile, and is deeply health promoting. Yet, today, physical inactivity is an unsolved, major public health problem. However, globally, tens of millions of people annually undertake ancient, significant and enduring traditions of physiologically and psychologically arduous walks (pilgrimages) of days-to-weeks extent. Pilgrim walking is a significant human activity requiring weighty commitments of time, action and belief, as well as community support. Paradoxically, human walking is most studied on treadmills, not ‘in the wild’, while mechanistically vital, treadmill studies of walking cannot, in principle, address why humans walk extraordinary distances together to demonstrate their adherence to a behaviourally demanding belief system.

Pilgrim walkers provide a rich ‘living laboratory’ bridging humanistic inquiries, to progressive theoretical and empirical investigations of human walking arising from a behaviourally demanding belief system. Pilgrims vary demographically and undertake arduous journeys on precisely mapped routes of tracked, titrated doses and durations on terrain of varying difficulty, allowing investigations from molecular to cultural levels of analysis. Using the reciprocal perspectives of ‘inside→out’ (where processes within brain and body initiate, support and entrain movement) and ‘outside→in’ (where processes in the world beyond brain and body drive activitywithinbrain and body), we examine how pilgrim walking might shape personal, social and transcendental processes, revealing potential mechanisms supporting the body and brain in motion, to how pilgrim walking might offer policy solutions for physical inactivity.
Relationship Between Parent Vowel Hyperarticulation in Infant-Directed Speech and Infant Phonetic Complexity on the Level of Conversational Turns
Authors: Marklund Ulrika , Marklund Ellen , Gustavsson Lisa
Published: August 2021

When speaking to infants, parents typically use infant-directed speech, a speech register that in several aspects differs from that directed to adults. Vowel hyperarticulation, that is, extreme articulation of vowels, is one characteristic sometimes found in infant-directed speech, and it has been suggested that there exists a relationship between how much vowel hyperarticulation parents use when speaking to their infant and infant language development. In this study, the relationship between parent vowel hyperarticulation and phonetic complexity of infant vocalizations is investigated. Previous research has shown that on the level of subject means, a positive correlational relationship exists. However, the previous findings do not provide information about the directionality of that relationship. In this study the relationship is investigated on a conversational turn level, which makes it possible to draw conclusions on whether the behavior of the infant is impacting the parent, the behavior of the parent is impacting the infant, or both. Parent vowel hyperarticulation was quantified using the vhh-index, a measure that allows vowel hyperarticulation to be estimated for individual vowel tokens. Phonetic complexity of infant vocalizations was calculated using the Word Complexity Measure for Swedish. Findings were unexpected in that a negative relationship was found between parent vowel hyperarticulation and phonetic complexity of the immediately following infant vocalization. Directionality was suggested by the fact that no such relationship was found between infant phonetic complexity and vowel hyperarticulation of the immediately following parent utterance. A potential explanation for these results is that high degrees of vowel hyperarticulation either provide, or co-occur with, large amounts of phonetic and/or linguistic information, which may occupy processing resources to an extent that affects production of the next vocalization.
Levels of Reading Comprehension in Higher Education: Systematic Review and Meta-Analysis
Authors: de-la-Peña Cristina , Luque-Rojas María Jesús
Published: August 2021

Higher education aims for university students to produce knowledge from the critical reflection of scientific texts. Therefore, it is necessary to develop a deep mental representation of written information. The objective of this research was to determine through a systematic review and meta-analysis the proportion of university students who have an optimal performance at each level of reading comprehension. Systematic review of empirical studies has been limited from 2010 to March 2021 using the Web of Science, Scopus, Medline, and PsycINFO databases. Two reviewers performed data extraction independently. A random-effects model of proportions was used for the meta-analysis and heterogeneity was assessed with I2. To analyze the influence of moderating variables, meta-regression was used and two ways were used to study publication bias. Seven articles were identified with a total sample of the seven of 1,044. The proportion of students at the literal level was 56% (95% CI = 39–72%, I2 = 96.3%), inferential level 33% (95% CI = 19–46%, I2 = 95.2%), critical level 22% (95% CI = 9–35%, I2 = 99.04%), and organizational level 22% (95% CI = 6–37%, I2 = 99.67%). Comparing reading comprehension levels, there is a significant higher proportion of university students who have an optimal level of literal compared to the rest of the reading comprehension levels. The results have to be interpreted with caution but are a guide for future research.
Teacher Well-Being in EFL/ESL Classrooms
Authors: Li Zhong
Published: August 2021

A popular point in the last 20 years in academic and business settings is well-being at work that is in line with positive psychology, through which one can understand how to make working conditions enjoyable. Alternatively, teaching has been recognized as the most stressful career. Although, not many studies in the form of review have been carried out to focus on the notion of the well-being of teachers in English as a second language (ESL) or English as a foreign language (EFL). According to the literature review, the definition of this construct, the factors related to it, and the empirical studies in this domain are presented. In conclusion, the implications of well-being for teachers, school principals, teacher-trainers, and future researchers are provided, and new directions for future research are delineated.
A Structural Equation Model of Health-Related Quality of Life in Chinese Patients With Rheumatoid Arthritis
Authors: Shen Biyu , Chen Haoyang , Yang Dongliang , Yolanda Ogbolu , Yuan Changrong , Du Aihua , Xu Rong , Geng Yaqin , Chen Xin , Li Huiling , Xu Guang-Yin
Published: August 2021

Background: The aim of this study was to examine how body image, Disease Activity Score in 28 joints, the feeling of being anxious, depression, fatigue, quality of sleep, and pain influence the quality of life (QoL) in patients with rheumatoid arthritis (RA).

Methods: A multicenter cross-sectional survey with convenience sampling was conducted from March 2019 and December 2019, 603 patients with RA from five hospitals were evaluated using the Body Image Disturbance Questionnaire, Disease Activity Score in 28 joints, Hospital Anxiety and Depression Scale, Fatigue Severity Scale, Pittsburgh Sleep Quality Index, Short Form 36 Health Survey, and Global Pain Scale. The relationship between quality of life and other variables was evaluated by using the structural equation model (SEM).

Results: A total of 580 patients were recruited. SEM fitted the data very well with a root mean square error of approximation (RMSEA) of 0.072. Comparative fit index of 0.966, and Tucker-Lewis index of 0.936. The symptoms and the normalized factor load of six variables showed that the normalized factor load of pain was 0.99.

Conclusions: The QoL model was used to fit an SEM to systematically verify and analyze the population disease data, biological factors, and the direct and indirect effects of the symptom group on the QoL, and the interactions between the symptoms. Therefore, the diagnosis, treatment and rehabilitation of RA is a long-term, dynamic, and complex practical process. Patients' personal symptoms, needs, and experiences also vary greatly. Comprehensive assessment of patients' symptoms, needs, and experiences, as well as the role of social support cannot be ignored, which can help to meet patients' nursing needs, improve their mood and pain-based symptom management, and ultimately improve patients' QoL.
The Amplifying Effect of Conflicts on Case Fatality Rate of COVID-19: Evidence From 120 Countries
Authors: Zhai Yonghui , Jiang Dayang , Gozgor Giray , Cho Eunho
Published: August 2021

Using the COVID-19 database of Johns Hopkins University, this study examines the determinants of the case fatality rate of COVID-19. We consider various potential determinants of the mortality risk of COVID-19 in 120 countries. The Ordinary Least Squares (OLS) and the Kernel-based Regularized Least Squares (KRLS) estimations show that internal and external conflicts are positively related to the case fatality rates. This evidence is robust to the exclusion of countries across different regions. Thus, the evidence indicates that conflict may explain significant differences in the case fatality rate of COVID-19 across countries.
Clinical Features and Survival Outcome of Early-Stage Primary Pulmonary MALT Lymphoma After Surgical Treatment
Authors: Xu Ye , Zheng Min , Guo Qingkui , Wang Ning , Wang Rui
Published: August 2021

Background: We aimed to study the clinical features and survival outcomes of patients with early-stage primary pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma who underwent surgery.

Methods: This is a retrospective, single-center study including 32 patients with early-stage primary pulmonary MALT lymphoma. Univariate and multivariate Cox analyses were performed to select independent prognostic factors. The overall survival (OS) was analyzed by the Kaplan-Meier method and was compared with the log-rank test.

Results: Among the 32 patients included, there were 16 men (50.0%) and 16 women (50.0%). The average age was 59.2 years old. Ten patients had non-specific clinical symptoms including cough, expectoration, and chest pain, and four patients had B symptoms. CT images are not specific and can be shown as peripheral, central, solid, and ground glass but more peripheral (93.8%) and solid (75.0%). In prognostic analysis, univariate analysis showed that tumor stage and size were associated with relapse-free survival (RFS) and OS [hazard ratio (HR) = 1.105, 95% CI: 1.021–1.197, P = 0.011; HR = 1.211, 95% CI: 1.158–1.968, P = 0.003, respectively]. It seems to indicate that higher stage and larger size indicate a worse prognosis, but we could not find statistically significant predictors in multivariate analysis. Sublobectomy was performed in 21 (65.6) cases, lobectomy was performed in the other 11 (34.4) cases, both of them can achieve good prognosis (5-year RFS and OS are both 100%), and there is no significant difference between them.

Conclusions: The clinical manifestation of early-stage primary pulmonary MALT lymphoma is not significantly specific, and surgical resection is an effective treatment.
Psychoacoustic Analysis of Vacuum Cleaner Noise
Authors: Kumar Sanjay , Wing Wong , Lee Heow
Published: August 2021

Vacuum cleaners are one of the most widely used household appliances associated with unpleasant noises. Previous studies have indicated the severity of loud vacuum cleaner noise and its impact on the users nearby. The standalone quantified measurements of the generated noise are not sufficient for properly characterizing vacuum cleaners. Human perception should also be included for a better assessment of the quality of sound. A hybrid approach such as psychoacoustics analysis, which comprises subjective and objective evaluations of sounds, has recently been widely used. This paper focuses on the experimental assessment of vacuum cleaner noise and evaluates their psychoacoustical matrices. Three vacuum cleaners with different specifications have been selected as test candidates, and their sound qualities have been analyzed. Statistical analysis, ANOVA, has been performed in order to investigate the effectiveness of individual psychoacoustic metrics.
Bi-Directional Origami-Inspired SMA Folding Microactuator
Authors: Seigner Lena , Tshikwand Georgino Kaleng , Wendler Frank , Kohl Manfred
Published: August 2021

We present the design, fabrication, and characterization of single and antagonistic SMA microactuators allowing for uni- and bi-directional self-folding of origami-inspired devices, respectively. Test devices consist of two triangular tiles that are interconnected by double-beam-shaped SMA microactuators fabricated from thin SMA foils of 20 µm thickness with memory shapes set to a 180° folding angle. Bi-directional self-folding is achieved by combining two counteracting SMA microactuators. We present a macromodel to describe the engineering stress–strain characteristics of the SMA foil and to perform FEM simulations on the characteristics of self-folding and the corresponding local evolution of phase transformation. Experiments on single-SMA microactuators demonstrate the uni-directional self-folding and tunability of bending angles up to 180°. The finite element simulations qualitatively describe the main features of the observed torque-folding angle characteristics and provide further insights into the angular dependence of the local profiles of the stress and martensite phase fraction. The first antagonistic SMA microactuators reveal bi-directional self-folding in the range of −44° to +40°, which remains well below the predicted limit of ±100°.
Incomplete Information Pursuit-Evasion Game Control for a Space Non-Cooperative Target
Authors: Wang Ziwen , Gong Baichun , Yuan Yanhua , Ding Xin
Published: August 2021

Aiming to solve the optimal control problem for the pursuit-evasion game with a space non-cooperative target under the condition of incomplete information, a new method degenerating the game into a strong tracking problem is proposed, where the unknown target maneuver is processed as colored noise. First, the relative motion is modeled in the rotating local vertical local horizontal (LVLH) frame originated at a virtual Chief based on the Hill-Clohessy-Wiltshire relative dynamics, while the measurement models for three different sensor schemes (i.e., single LOS (line-of-sight) sensor, LOS range sensor and double LOS sensor) are established and an extended Kalman Filter (EKF) is used to obtain the relative state of target. Next, under the assumption that the unknown maneuver of the target is colored noise, the game control law of chaser is derived based on the linear quadratic differential game theory. Furthermore, the optimal control law considering the thrust limitation is obtained. After that, the observability of the relative orbit state is analyzed, where the relative orbit is weakly observable in a short period of time in the case of only LOS angle measurements, fully observable in the cases of LOS range and double LOS measurement schemes. Finally, numerical simulations are conducted to verify the proposed method. The results show that by using the single LOS scheme, the chaser would firstly approach the target but then would lose the game because of the existence of the target’s unknown maneuver. Conversely, the chaser can successfully win the game in the cases of LOS range and double LOS sensor schemes.
Proximal Sensing in Grasslands and Pastures
Authors: Ghajar Shayan , Tracy Benjamin
Published: August 2021

Reliable measures of biomass, species composition, nitrogen status, and nutritive value provide important indicators of the status of pastures and rangelands, allowing managers to make informed decisions. Traditional methods of sample collection necessitate significant investments in time and labor. Proximal sensing technologies have the potential to collect more data with a smaller investment in time and labor. However, methods and protocols for conducting pasture assessments with proximal sensors are still in development, equipment and software vary considerably, and the accuracy and utility of these assessments differ between methods and sites. This review summarizes the methods currently being developed to assess pastures and rangelands worldwide and discusses these emerging technologies in the context of diffusion of innovation theory.
Management of Phosphorus in Salinity-Stressed Agriculture for Sustainable Crop Production by Salt-Tolerant Phosphate-Solubilizing Bacteria—A Review
Authors: Dey Gobinda , Banerjee Pritam , Sharma Raju Kumar , Maity Jyoti Prakash , Etesami Hassan , Shaw Arun Kumar , Huang Yi-Hsun , Huang Hsien-Bin , Chen Chien-Yen
Published: August 2021

Among the environmental factors, soil salinity is one of the most detrimental factors affecting plant growth and productivity. Nutritional-imbalance is also known as one of the negative effects of salinity on plant growth and productivity. Among the essential plant nutrients, phosphorus (P) is a nutrient in which the uptake, transport, and distribution in plant is adversely affected by salinity-stress. Salinity-stress-mediated low a P availability limits the crop production. Adding additional P fertilizer is generally recommended to manage P deficit in saline-soils; however, the low-efficiency of available P fertilizer use in salt-affected soils, restricts P availability, and P fertilizers are also a cause of significant environmental concerns. The application of salinity-tolerant phosphate–solubilizing-bacteria (ST-PSB) can be as a greatly effective and economical way to improve the P availability, and recover the P-deficit in saline-land. This review focuses on soil salinization and its effect on P availability, the mechanisms of P solubilization by ST-PSB, ST-PSB diversity, their role in alleviating salinity stress in plants, the current and future scenarios of their use, and the potential application of this knowledge to manage the sustainable environmental system. According to this review, adding ST-PSB to saline soils could be an alternative for alleviating the negative effects of salinity on plants and may ameliorate salinity tolerance.
The Sensitivity of Field Populations of Metopolophium dirhodum (Walker) (Hemiptera: Aphididae) to Seven Insecticides in Northern China
Authors: Gong Peipan , Li Xinan , Wang Chao , Zhu Saige , Li Qiuchi , Zhang Yunhui , Li Xiangrui , Li Guangkuo , Liu Enliang , Gao Haifeng , Yang Xueqing , Zhu Xun
Published: August 2021

Insect pests are primarily controlled by insecticides. However, the sensitivity decreases and insecticide resistance is problematic for the effective management of agriculturally important insects, including Metopolophium dirhodum, which is an aphid that commonly feeds on cereals. The insecticide sensitivity status and potential resistance of M. dirhodum field populations remain relatively unknown. In this study, the susceptibility of 19 M. dirhodum populations from seven provinces in Northern China to neonicotinoids, pyrethroids, organophosphates, and a macrolide (abamectin) was determined in 2017–2019. The results indicated that two populations were highly resistant to thiamethoxam, with a relative resistance ratio (RLR) of 134.03 and 103.03, whereas one population was highly resistant to beta-cypermethrin (RLR of 121.42). On the basis of the RLR, the tested M. dirhodum populations ranging from susceptible to showing moderate levels of resistance to imidacloprid (RLR of 1.50 to 57.29), omethoate (RLR of 1.07 to 18.73), and abamectin (RLR of 1.10 to 25.89), but they were ranging from susceptible to showing tolerance or low levels of resistance to bifenthrin (RLR of 1.14 to 6.02) and chlorpyrifos (RLR of 1.11 to 7.59). Furthermore, a pair-wise correlation analysis revealed a significant correlation between the median lethal concentrations (LC50) for beta-cypermethrin and thiamethoxam, reflecting the cross-resistance between these two insecticides. The data obtained in our study provide timely information about aphid insecticide sensitivity, which may be used to delay the evolution of M. dirhodum insecticide resistance in Northern China.
Effects of Dietary Rosemary Extract Supplementation on Pork Quality of Chato Murciano Breed during Storage
Authors: Peñaranda Irene , Auqui Sonia Mariella , Egea Macarena , Linares María Belén , Garrido María Dolores
Published: August 2021

(1) Background: The effect of Rosmarinus officinalis L. dietary supplementation on the pork meat quality of the Chato Murciano breed of pigs was evaluated during 21 days of storage. (2) Methods: Twenty-one castrated male pigs were divided into two groups. One group was fed a control diet (group C), and the other group consumed the same diet plus a 1000 ppm supplement of deodorized rosemary extract (group R). (3) Results: While the inclusion of rosemary extract in the pig diet did not produce notable changes in the technological parameters analysed in the meat, the lower microbiological count obtained in meat pointed to the antimicrobial effect of the extract. The storage time had a significant effect on all the parameters studied in both groups (C and R). Thus, lipid oxidation increased and the colour of the meat deteriorated, at the same time as the microbial counts and the deterioration of the sensory attributes increased. (4) Conclusions: Therefore, a certain antimicrobial effect of rosemary was observed in the meat of Chato Murciano.
Physiological Parameters to Identify Suitable Blood Donor Cows for Preparation of Platelet Rich Plasma
Authors: Lange-Consiglio Anna , Garlappi Rosangela , Spelta Chiara , Idda Antonella , Comazzi Stefano , Rizzi Rita , Cremonesi Fausto
Published: August 2021

Platelet rich plasma (PRP) has been shown to be beneficial in the treatment of bovine mastitis, with an action comparable to that of antibiotics. Autologous treatment is feasible in experimental conditions but is difficult to apply in field conditions, particularly in acute mastitis. The ideal scenario would be to have heterologous PRP stored on every farm so that it is readily available when needed. In this paper, we analysed data collected during bovine mastitis treatment with heterologous PRP produced by casual donor cows on several farms. We tried to identify parameters which might be useful to identify the most suitable cows to be used as blood donors, to obtain the highest yield of PRP. Variables considered for each animal were the age, the parity, the date of the last parturition, the season of blood collection, the site of blood collection (jugular or mammary vein) and the reproductive status e.g., pregnant or not pregnant. There were statistically significant differences for all the variables considered from the 135 blood cows, except for the blood collection season. The highest yield of PRP was associated with nonpregnancy blood collection within three months of parturition, parity 3 or 4, and blood collection from the mammary vein.
Correction: Carnovale, F.; Phillips, C.J.C. The Effects of Heat Stress on Sheep Welfare during Live Export Voyages from Australia to the Middle East. Animals 2020, 10, 694
Authors: Carnovale Francesca , Phillips Clive J. C.
Published: August 2021

The authors wish to make the following correction to this paper [...]
Development and Validation of a Test for the Classification of Horses as Broken or Unbroken
Authors: Menchetti Laura , Dalla Costa Emanuela , Minero Michela , Padalino Barbara
Published: August 2021

Regulation EC 1/2005 has stricter rules for transportation of unbroken (untamed) vs. broken (tamed) horses, but does not provide adequate tools for their identification. This study aimed to develop and validate such a tool. A behavioural test (Broken/Unbroken Test (BUT)) based on approaching, haltering, and leading was applied to 100 horses. Physiological and additional behavioural data were also collected, and the horses’ status (broken/unbroken) was assessed by the expert who administered the BUT. Each horse’s behaviour during the BUT was scored by four trained observers blinded to the horse’s history. The BUT score showed excellent inter-observer, intra-observer, and test–retest reliability (all intraclass correlation coefficients (ICCs) > 0.75). It was also negatively associated with respiratory rate, avoidance distance, and time needed to approach, halter, and lead the horse (p < 0.05 for all). The optimal BUT score cut-off for discrimination between broken and unbroken horses (gold standard: expert judgment) showed 97.8% sensitivity and 97.3% specificity. There was almost perfect agreement between BUT-based and expert classification of horses (ICC = 0.940). These findings confirm the BUT’s construct and criterion validity. The BUT could provide officials with a feasible, reliable, and valid tool to identify a horse’s broken/unbroken status and, consequently, direct stakeholders towards correct transport procedures.
Bacterial Nosocomial Infections: Multidrug Resistance as a Trigger for the Development of Novel Antimicrobials
Authors: Sousa Sílvia A. , Feliciano Joana R. , Pita Tiago , Soeiro Catarina F. , Mendes Beatriz L. , Alves Luis G. , Leitão Jorge H.
Published: August 2021

Nosocomial bacterial infections are associated with high morbidity and mortality, posing a huge burden to healthcare systems worldwide. The ongoing COVID-19 pandemic, with the raised hospitalization of patients and the increased use of antimicrobial agents, boosted the emergence of difficult-to-treat multidrug-resistant (MDR) bacteria in hospital settings. Therefore, current available antibiotic treatments often have limited or no efficacy against nosocomial bacterial infections, and novel therapeutic approaches need to be considered. In this review, we analyze current antibacterial alternatives under investigation, focusing on metal-based complexes, antimicrobial peptides, and antisense antimicrobial therapeutics. The association of new compounds with older, commercially available antibiotics and the repurposing of existing drugs are also revised in this work.
Peroxiredoxins as Potential Targets for Cardiovascular Disease
Authors: Jeong Se-Jin , Park Jong-Gil , Oh Goo Taeg
Published: August 2021

Increased oxidative stress (OS) is considered a common etiology in the pathogenesis of cardiovascular disease (CVD). Therefore, the precise regulation of reactive oxygen species (ROS) in cardiovascular cells is essential to maintain normal physiological functions. Numerous regulators of cellular homeostasis are reportedly influenced by ROS. Hydrogen peroxide (H2O2), as an endogenous ROS in aerobic cells, is a toxic substance that can induce OS. However, many studies conducted over the past two decades have provided substantial evidence that H2O2 acts as a diffusible intracellular signaling messenger. Antioxidant enzymes, including superoxide dismutases, catalase, glutathione peroxidases, and peroxiredoxins (Prdxs), maintain the balance of ROS levels against augmentation of ROS production during the pathogenesis of CVD. Especially, Prdxs are regulatory sensors of transduced intracellular signals. The intracellular abundance of Prdxs that specifically react with H2O2 act as regulatory proteins. In this review, we focus on the role of Prdxs in the regulation of ROS-induced pathological changes in the development of CVD.
A Comparison of Manual Nylon Bristle Toothbrushes versus Thermoplastic Elastomer Toothbrushes in Terms of Cleaning Efficacy and the Biological Potential Role on Gingival Health
Authors: Ballini Andrea , Di Cosola Michele , Saini Rajiv , Benincasa Caterina , Aiello Elisabetta , Marrelli Benedetta , Rajiv Saini Sugandha , Ceruso Francesco Mattia , Nocini Riccardo , Topi Skender , Bottalico Lucrezia , Pettini Francesco , Cantore Stefania
Published: August 2021

Background: It is well-known that toothbrushing might be associated with the development of oral soft tissue lesions. There is currently a continuing increase in the demand for new safety and performing materials in daily homecare oral hygiene including soft and extra-soft toothbrush bristles that tend to be safer. The aim of this study was to compare the efficacy of plaque control and the potential effects on gingival health of two different toothbrush bristle models. Methods: In a three-month period, a total of forty subjects were evaluated for Oral Hygiene Index (OHI), Gingival Index (GI) and Plaque Index (PI) scores as well the Gingival Abrasion Assessment (GAA) between a toothbrush entirely made from a rubber-like material called thermoplastic elastomer (TPE) and a soft toothbrush (standard control with nylon bristles) in a clinical, single-blind, controlled, parallel-group trial. Results: The use of the TPE toothbrush allows a reduction in the PI, improves the OHI and modifies the GAA in the TPE group over a period of three months compared with a conventional soft bristle toothbrush applied for the same period, leading in this way evidence for a good influence of the TPE bristles on overall oral hygiene conditions. Conclusions: From the comparison between our data and the literature studies, we can state that the material and shape of the bristles of the toothbrush affect the home practice of oral hygiene. The TPE bristles reduce the presence of plaque formation and gingival bleeding, oral soft tissue injuries acquired during homecare oral hygiene.