bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of Soil Nutrient Contents Using Visible and Near-Infrared Reflectance Spectroscopy
Authors: Peng Yiping , Zhao Li , Hu Yueming , Wang Guangxing , Wang Lu , Liu Zhenhua
Published: October 2019

Quickly and efficiently monitoring soil nutrient contents using remote sensing technology is of great significance for farmland soil productivity, food security and sustainable agricultural development. Current research has been conducted to estimate and map soil nutrient contents in large areas using hyper-spectral techniques, however, it is difficult to obtain accurate estimates. In order to improve the estimation accuracy of soil nutrient contents, we introduced a GA-BPNN method, which combined a back propagation neural network (BPNN) with the genetic algorithm optimization (GA). This study was conducted in Guangdong, China, based on soil nutrient contents and hyperspectral data. The prediction accuracies from a partial least squares regression (PLSR), BPNN and GA-BPNN were compared using field observations. The results showed that (1) Among three methods, the GA-BPNN provided the most accurate estimates of soil total nitrogen (TN), total phosphorus (TP) and total potassium (TK) contents; (2) Compared with the BPNN models, the GA-BPNN models significantly improved the estimation accuracies of the soil nutrient contents by decreasing the relative root mean square error (RRMSE) values by 15.9%, 5.6% and 20.2% at the sample point level, and 20.1%, 16.5% and 47.1% at the regional scale for TN, TP and TK, respectively. This indicated that by optimizing the parameters of BPNN, the GA-BPNN provided greater potential to improving the estimation; and (3) Soil TK content could be more accurately mapped by the GA-BPNN method using HuanJing-1A Hyperspectral Imager (HJ-1A HSI) (manufacturer: China Aerospace Science and Technology Corporation; Beijing, China) data with a RRMSE value of 20.37% than the soil TN and TP with the RRMSE values of 40.41% and 34.71%, respectively. This implied that the GA-BPNN model provided the potential to map the soil TK content for the large area. The research results provided an important reference for high-accuracy prediction of soil nutrient contents.
Identification and Mapping of Soil Erosion Processes Using the Visual Interpretation of LiDAR Imagery
Authors: Đomlija Petra , Bernat Gazibara Sanja , Arbanas Željko , Mihalić Arbanas Snježana
Published: October 2019

Soil erosion processes are a type of geological hazard. They cause soil loss and sediment production, landscape dissection, and economic damage, which can, in the long term, result in land abandonment. Thus, identification of soil erosion processes is necessary for sustainable land management in an area. This study presents the potential of visual interpretation of high resolution LiDAR (light detection and ranging) imagery for direct and unambiguous identification and mapping of soil erosion processes, which was tested in the study area of the Vinodol Valley (64.57 km2), in Croatia. Eight LiDAR images were derived from the 1 m airborne LiDAR DTM (Digital Terrain Model) and were used to identify and map gully erosion, sheet erosion, and the combined effect of rill and sheet erosion, with the ultimate purpose to create a historical erosion inventory. The two-step procedure in a visual interpretation of LiDAR imagery was performed: preliminary and detailed. In the preliminary step, possibilities and limitations for unambiguous identification of the soil erosion processes were determined for representative portions of the study area, and the exclusive criteria for the accurate and precise manual delineation of different types of erosion phenomena were established. In the detailed step, the findings from the preliminary step were used to map the soil erosion phenomena in the entire studied area. Results determined the highest potential for direct identification and mapping of the gully erosion phenomena. A total of 236 gullies were identified and precisely delineated, although most of them were previously unknown, due to the lack of previous investigations on soil erosion processes in the study area. On the other hand, the used method was proven to be inapplicable for direct identification and accurate mapping of the sheet erosion. Sheet erosion, however, could have been indirectly identified on certain LiDAR imagery, based on recognition of colluvial deposits accumulated at the foot of the eroded slopes. Furthermore, the findings of this study present which of the used LiDAR imagery, and what features of the imagery used, are most effective for identification and mapping of different types of erosion processes.
Stabilization of c-KIT G-Quadruplex DNA Structures by the RNA Polymerase I Inhibitors BMH-21 and BA-41
Authors: Mazzini Stefania , Gargallo Raimundo , Musso Loana , De Santis Francesca , Aviñó Anna , Scaglioni Leonardo , Eritja Ramon , Di Nicola Massimo , Zunino Franco , Amatulli Annabella , Dallavalle Sabrina
Published: October 2019

The stabilization of G-quadruplex DNA structures by small molecules with affinity to oncogene promoters has emerged as a promising anticancer strategy, due to a potential role in gene expression regulation. We explored the ability of BMH-21 (1) and its analogue BA-41 (2) to bind the G-quadruplex structure present in the c-KIT promoter by biophysical methods and molecular modeling. We provide evidence that both compounds interact with the c-KIT 21-mer sequence. The stable monomeric intramolecular parallel G-quadruplex obtained by the mutation of positions 12 and 21 allowed the precise determination of the binding mode by NMR and molecular dynamics studies. Both compounds form a complex characterized by one ligand molecule positioned over the tetrad at the 3′-end, stabilized by an extensive network of π–π interactions. The binding constants (Kb) obtained with fluorescence are similar for both complexes (around 106 M−1). Compound BA-41 (2) showed significant antiproliferative activity against a human lymphoma cell line, SU-DHL4, known to express substantial levels of c-KIT. However, the partial inhibition of c-KIT expression by Western blot analysis suggested that the interaction of compound 2 with the c-KIT promoter is not the primary event and that multiple effects provide a contribution as determinants of biological activity.
Prognostic Factors and Biomarkers of Responses to Immune Checkpoint Inhibitors in Lung Cancer
Authors: Bianco Andrea , Perrotta Fabio , Barra Giusi , Malapelle Umberto , Rocco Danilo , De Palma Raffaele
Published: October 2019

Manipulation of the immune response is a game changer in lung cancer treatment, revolutionizing management. PD1 and CTLA4 are dynamically expressed on different T cell subsets that can either disrupt or sustain tumor growth. Monoclonal antibodies (MoAbs) against PD1/PDL1 and CTLA4 have shown that inhibitory signals can be impaired, blocking T cell activation and function. MoAbs, used as both single-agents or in combination with standard therapy for the treatment of advanced non-small cell lung cancer (NSCLC), have exhibited advantages in terms of overall survival and response rate; nivolumab, pembrolizumab, atezolizumab and more recently, durvalumab, have already been approved for lung cancer treatment and more compounds are in the pipeline. A better understanding of signaling elicited by these antibodies on T cell subsets, as well as identification of biological determinants of sensitivity, resistance and correlates of efficacy, will help to define the mechanisms of antitumor responses. In addition, the relevance of T regulatory cells (Treg) involved in immune responses in cancer is attracting increasing interest. A major challenge for future research is to understand why a durable response to immune checkpoint inhibitors (ICIs) occurs only in subsets of patients and the mechanisms of resistance after an initial response. This review will explore current understanding and future direction of research on ICI treatment in lung cancer and the impact of tumor immune microenvironment n influencing clinical responses.
Transforming Growth Factor-β1 Selectively Recruits microRNAs to the RNA-Induced Silencing Complex and Degrades CFTR mRNA under Permissive Conditions in Human Bronchial Epithelial Cells
Authors: Mitash Nilay , Mu Fangping , Donovan Joshua E. , Myerburg Michael M. , Ranganathan Sarangarajan , Greene Catherine M. , Swiatecka-Urban Agnieszka
Published: October 2019

Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene lead to cystic fibrosis (CF). The most common mutation F508del inhibits folding and processing of CFTR protein. FDA-approved correctors rescue the biosynthetic processing of F508del-CFTR protein, while potentiators improve the rescued CFTR channel function. Transforming growth factor (TGF-β1), overexpressed in many CF patients, blocks corrector/potentiator rescue by inhibiting CFTR mRNA in vitro. Increased TGF-β1 signaling and acquired CFTR dysfunction are present in other lung diseases. To study the mechanism of TGF-β1 repression of CFTR, we used molecular, biochemical, and functional approaches in primary human bronchial epithelial cells from over 50 donors. TGF-β1 destabilized CFTR mRNA in cells from lungs with chronic disease, including CF, and impaired F508del-CFTR rescue by new-generation correctors. TGF-β1 increased the active pool of selected micro(mi)RNAs validated as CFTR inhibitors, recruiting them to the RNA-induced silencing complex (RISC). Expression of F508del-CFTR globally modulated TGF-β1-induced changes in the miRNA landscape, creating a permissive environment required for degradation of F508del-CFTR mRNA. In conclusion, TGF-β1 may impede the full benefit of corrector/potentiator therapy in CF patients. Studying miRNA recruitment to RISC under disease-specific conditions may help to better characterize the miRNAs utilized by TGF-β1 to destabilize CFTR mRNA.
L-Alanine Exporter, AlaE, of Escherichia coli Functions as a Safety Valve to Enhance Survival under Feast Conditions
Authors: Katsube Satoshi , Ando Tasuke , Yoneyama Hiroshi
Published: October 2019

The intracellular level of amino acids is determined by the balance between their anabolic and catabolic pathways. L-alanine is anabolized by three L-alanine synthesizing enzymes and catabolized by two racemases and D-amino acid dehydrogenase (DadA). In addition, its level is regulated by L-alanine movement across the inner membrane. We identified the novel gene alaE, encoding an L-alanine exporter. To elucidate the physiological function of L-Alanine exporter, AlaE, we determined the susceptibility of alaE-, dadA-, and alaE/dadA-deficient mutants, derived from the wild-type strain MG1655, to L-alanyl-L-alanine (Ala-Ala), which shows toxicity to the L-alanine-nonmetabolizing variant lacking alaE. The dadA-deficient mutant has a similar minimum inhibitory concentration (MIC) (>1.25 mg/mL) to that observed in MG1655. However, alaE- and alaE/dadA-deficient mutants had MICs of 0.04 and 0.0025 mg/mL, respectively. The results suggested that the efficacy of AlaE to relieve stress caused by toxic intracellular accumulation of L-alanine was higher than that of DadA. Consistent with this, the intracellular level of alanine in the alaE-mutant was much higher than that in MG1655 and the dadA-mutant. We, therefore, conclude that AlaE functions as a ‘safety-valve’ to prevent the toxic level accumulation of intracellular L-alanine under a peptide-rich environment, such as within the animal intestine.
Assessing Feeding Damage from Two Leaffooted Bugs, Leptoglossus clypealis Heidemann and Leptoglossus zonatus (Dallas) (Hemiptera: Coreidae), on Four Almond Varieties
Authors: Joyce Andrea L. , Barman Apurba K. , Doll David , Higbee Bradley S.
Published: October 2019

Leaffooted bugs (Leptoglossus spp; Hemiptera: Coreidae) are phytophagous insects native to the Western Hemisphere. In California, Leptoglossus clypealis and Leptoglossus zonatus are occasional pests on almonds. Early season feeding by L. clypealis and L. zonatus leads to almond drop, while late season feeding results in strikes on kernels, kernel necrosis, and shriveled kernels. A field cage study was conducted to assess feeding damage associated with L. clypealis and L. zonatus on four almond varieties, Nonpareil, Fritz, Monterey, and Carmel. The objectives were to determine whether leaffooted bugs caused significant almond drop, to pinpoint when the almond was vulnerable, and to determine the final damage at harvest. Branches with ~20 almonds were caged and used to compare almond drop and final damage in four treatments: (1) control branches, (2) mechanically punctured almonds, (3) adult Leptoglossus clypealis, and (4) adult Leptoglossus zonatus. Replicates were set up for eight weeks during two seasons. Early season feeding resulted in higher almond drop than late season, and L. zonatus resulted in greater drop than L. clypealis. The almond hull width of the four varieties in the study did not influence susceptibility to feeding damage. The final damage assessment at harvest found significant levels of kernel strikes, kernel necrosis, and shriveled almonds in bug feeding cages, with higher levels attributed to L. zonatus than L. clypealis. Further research is warranted to develop an Integrated Pest Management program with reduced risk controls for L. zonatus.
Multiple External Root Resorption of Teeth as a New Manifestation of Systemic Sclerosis—A Cross-Sectional Study in Japan
Authors: Memida Takumi , Matsuda Shinji , Kajiya Mikihito , Mizuno Noriyoshi , Ouhara Kazuhisa , Fujita Tsuyoshi , Hirata Shintaro , Yoshida Yusuke , Sugimoto Tomohiro , Nishi Hiromi , Kawaguchi Hiroyuki , Sugiyama Eiji , Kurihara Hidemi
Published: October 2019

Background: Multiple external root resorption (MERR) has been reported in systemic sclerosis (SSc) patients in Japan and Spain. To establish whether MERR is a new manifestation, we investigated the prevalence of MERR and systemic and oral manifestations to be associated with MERR in patients with SSc. Methods: Root resorption was detected by dental X-rays, panoramagraphy or cone beam computed tomography (CBCT). The prevalence of systemic and oral manifestations was examined by rheumatologists and dentists, respectively. Autoantibodies were investigated using laboratory tests. Results: MERR was detected in four out of the 41 patients (9.8%) who participated in the present study. The prevalence of digital ulcers was significantly higher in patients with MERR (MERR vs. non-MERR, 75% vs. 16.2%, p < 0.05), whereas that of other systemic manifestations was not. The prevalence of face skin sclerosis (100% vs. 10.8%, p < 0.01), calcinosis at the facial region (75% vs. 0%, p < 0.01), limited mouth opening (75% vs. 18.9% p < 0.05), temporomandibular disorder symptoms (50% vs. 2.7%, p < 0.05), and tongue rigidity (75% vs. 2.7%, p < 0.05) was significantly higher in patients with MERR. Conclusion: SSc patients with MERR had highly homogenous maxillofacial manifestations. Further clinical and basic studies are needed to elucidate the mechanisms underlying MERR in SSc patients.
Spectral Analysis of the QT Interval Increases the Prediction Accuracy of Clinical Variables in Brugada Syndrome
Authors: García-Iglesias Daniel , de Cos Francisco Javier , Romero Francisco Javier , Polana Srujana , Rubín José Manuel , Pérez Diego , Reguero Julián , de la Hera Jesús María , Avanzas Pablo , Gómez Juan , Coto Eliecer , Morís César , Calvo David
Published: October 2019

(1) Background: The clinical management of Brugada Syndrome (BrS) remains suboptimal. (2) Objective: To explore the role of standard electrocardiogram (ECG) spectral analysis in diagnosis and risk stratification. (3) Methods: We analyzed 337 patients—43 with a spontaneous type I ECG pattern (Spont-BrS), 112 drug induced (Induct-BrS), and 182 with a negative response to the drug challenge (negative responders (NR)). ECGs were processed using the wavelet transform (high frequency: 85 to 130 Hz). (4) Results: The power of the high-frequency content in the ST segment (Total ST Power; nV2Hz−1103) was higher in BrS compared with NR patients (Spont-BrS: 28.126 (7.274–48.978) vs. Induc-BrS: 26.635 (15.846–37.424) vs. NR: 11.13 (8.917–13.343); p = 0.002). No differences were observed between ECG patterns in BrS patients. However, the Total ST Power of the type II or III ECG in NR patients was lower than in the same ECG patterns recorded from BrS patients (BrS: 31.07 (16.856–45.283); vs. NR: 10.8 (7.248–14.352) nV2Hz−1103; p = 0.007). The Total ST Power, age, and family history of BrS were independent predictors of positive responses to drug testing. Comparing models with versus those without Total ST Power, the area under the received operator curve (ROC) curve increased (with 0.607 vs. without 0.528, p = 0.001). Only syncope was associated with an increased risk (follow-up 55.8 ± 39.35 months). However, the area under the ROC curve increased significantly when the Total ST Power was included as a covariate (with 0.784 vs. without 0.715, p = 0.04). (5) Conclusions: The analysis of the high-frequency content of ECG signals increases the predictive capability of clinical variables in BrS patients.
Follow-Up Recommendations after Curative Resection of Well-Differentiated Neuroendocrine Tumours: Review of Current Evidence and Clinical Practice
Authors: Lamarca Angela , Clouston Hamish , Barriuso Jorge , McNamara Mairéad G , Frizziero Melissa , Mansoor Was , Hubner Richard A , Manoharan Prakash , O’Dwyer Sarah , Valle Juan W
Published: October 2019

The incidence of neuroendocrine neoplasms (NENs) is increasing, especially for patients with early stages and grade 1 tumours. Current evidence also shows increased prevalence, probably reflecting earlier stage diagnosis and improvement of treatment options. Definition of adequate postsurgical follow-up for NENs is a current challenge. There are limited guidelines, and heterogeneity in adherence to those available is notable. Unfortunately, the population of patients at greatest risk of recurrence has not been defined clearly. Some studies support that for patients with pancreatic neuroendocrine tumours (PanNETs), factors such as primary tumour (T), stage, grade (Ki-67), tumour size, and lymph node metastases (N) are of relevance. For bronchial neuroendocrine tumours (LungNETs) and small intestinal neuroendocrine tumours (siNETs), similar factors have been identified. This review summarises the evidence supporting the rationale behind follow-up after curative resection in well-differentiated PanNETs, siNETs, and LungNETS. Published evidence informing relapse rate, disease-free survival, and relapse patterns are discussed, together with an overview of current guidelines informing postsurgical investigations and duration of follow-up.
Percutaneous Computed Tomography-Guided Coaxial Core Biopsy for the Diagnosis of Pancreatic Tumors
Authors: Su Yung-Yeh , Liu Yi-Sheng , Chao Ying-Jui , Chiang Nai-Jung , Yen Chia-Jui , Tsai Hong-Ming
Published: October 2019

Endoscopic, ultrasound-guided tissue acquisition (EUS-TA) with rapid on-site evaluation is recommended as a first choice in the diagnosis of pancreatic lesions. Since EUS facilities and rapid on-site evaluation are not widely available, even in medical centers, an alternative for precise diagnoses of pancreatic tumor is warranted. The percutaneous computed tomography-guided, core needle biopsy (CT-CNB) is a commonly applicable method for biopsies. Our institute has developed a fat-transversing approach for pancreatic biopsies which is able to approach most tumors in the pancreas without penetrating organs or vessels. Herein, we report a 15-year experiment of pancreatic tumor coaxial CT-CNB in 420 patients. The success rate of tissue yielding by the technique was 99.3%. The overall sensitivity, specificity, and accuracy were 93.2%, 100%, and 93.4%, respectively. The diagnostic accuracy could be increased to 96.4% in 2016–2018 (after the learning curve period). The overall complication rate was 8.6%. Neither life-threatening major complications, nor seeding through the biopsy tract, were observed. Our study supported the hypothesis that CT-CNB could be a complementary option for diagnostic tissue acquisition in patients with unresectable or metastatic pancreatic tumors when EUS-TA is either unsuitable or unavailable.
Elevated Hedgehog-Interacting Protein Levels in Subjects with Prediabetes and Type 2 Diabetes
Authors: Lin An-Chi , Hung Hao-Chang , Chen Yun-Wen , Cheng Kai-Pi , Li Chung-Hao , Lin Ching-Han , Chang Chih-Jen , Wu Hung-Tsung , Ou Horng-Yih
Published: October 2019

Background: The prevalence of diabetes is rapidly increasing worldwide and is highly associated with the incidence of cancers. In order to prevent diabetes, early diagnosis of prediabetes is important. However, biomarkers for prediabetes diagnosis are still scarce. The hedgehog-interacting protein (Hhip) is important in embryogenesis and is known to be a biomarker of several cancers. However, Hhip levels in subjects with diabetes are still unknown. Methods: In total, 314 participants were enrolled and divided into normal glucose tolerance (NGT; n = 75), impaired fasting glucose (IFG; n = 66), impaired glucose tolerance (IGT; n = 86), and newly diagnosed diabetes (NDD; n = 87) groups. Plasma Hhip levels were determined by an ELISA. The association between the Hhip and the presence of diabetes was examined by a multivariate linear regression analysis. Results: There were significant differences in the body mass index, systolic and diastolic blood pressure, fasting plasma glucose (FPG), post-load 2-h glucose, hemoglobin A1c (A1C), C-reactive protein, total cholesterol, triglyceride, and high- and low-density lipoprotein cholesterol levels among the groups. Concentrations of the Hhip were 2.45 ± 2.12, 4.40 ± 3.22, 4.44 ± 3.64, and 6.31 ± 5.35 ng/mL in subjects in the NGT, IFG, IGT, and NDD groups, respectively. In addition, we found that A1C and FPG were independently associated with Hhip concentrations. Using NGT as a reference group, IFG, IGT, and NDD were all independently associated with Hhip concentrations. Conclusions: Hhip was positively associated with prediabetes and type 2 diabetes mellitus.
Management Strategies for Antidepressant-Related Sexual Dysfunction: A Clinical Approach
Authors: Montejo Angel , Prieto Nieves , de Alarcón Rubén , Casado-Espada Nerea , de la Iglesia Javier , Montejo Laura
Published: October 2019

Major depressive disorder is a serious mental disorder in which treatment with antidepressant medication is often associated with sexual dysfunction (SD). Given its intimate nature, treatment emergent sexual dysfunction (TESD) has a low rate of spontaneous reports by patients, and this side effect therefore remains underestimated in clinical practice and in technical data sheets for antidepressants. Moreover, the issue of TESD is rarely routinely approached by clinicians in daily praxis. TESD is a determinant for tolerability, since this dysfunction often leads to a state of patient distress (or the distress of their partner) in the sexually active population, which is one of the most frequent reasons for lack of adherence and treatment drop-outs in antidepressant use. There is a delicate balance between prescribing an effective drug that improves depressive symptomatology and also has a minimum impact on sexuality. In this paper, we detail some management strategies for TESD from a clinical perspective, ranging from prevention (carefully choosing an antidepressant with a low rate of TESD) to possible pharmacological interventions aimed at improving patients’ tolerability when TESD is present. The suggested recommendations include the following: for low sexual desire, switching to a non-serotoninergic drug, lowering the dose, or associating bupropion or aripiprazole; for unwanted orgasm delayal or anorgasmia, dose reduction, “weekend holiday”, or switching to a non-serotoninergic drug or fluvoxamine; for erectile dysfunction, switching to a non-serotoninergic drug or the addition of an antidote such as phosphodiesterase 5 inhibitors (PD5-I); and for lubrication difficulties, switching to a non-serotoninergic drug, dose reduction, or using vaginal lubricants. A psychoeducational and psychotherapeutic approach should always be considered in cases with poorly tolerated sexual dysfunction.
Effects of Different Temperatures on the Softening of Red-Bed Sandstone in Turbulent Flow
Authors: Liu Zhen , He Xinfu , Cui Guangjun , Zhou Cuiying
Published: October 2019

The rates of chemical reactions are highly dependent on temperature, meaning that the actual geological rock mass is affected by different temperatures. Only when the temperature effect is considered can the mechanism of the influence of temperature on the interaction between water and rock be further understood. It was found that the condition of turbulent flow is more likely to promote the softening of red-bed sandstone than the conditions of laminar flow and static water in an experimental study on the softening effects of different flow patterns on red-bed sandstone. Therefore, based on a multi-functional self-circulating open channel hydraulic test system, this paper designs and completes equal volume saturated tests of red-bed sandstone at low temperature (1 °C), medium temperature (23 °C), and high temperature (45 °C) under the turbulent conditions of three equal temperature gradients. The chemical action of the circulating solution in water flow at different temperatures, the propagation of micro-cracks in rock and the changes in mechanical indexes are discussed. The influence laws and mechanisms of the different temperatures on the softening of red-bed sandstone in turbulent flow are revealed. The results show that low-temperature flow can inhibit the softening of red-bed sandstone in the range of 1–45 °C. With the increase in water flow temperature, the development degrees of micro-structures and the mechanical damage of the corresponding rock become more notable. That is, temperature affects the physical and chemical water-rock interactions and then changes the internal structure of rock, thus affecting the softening and failure processes of red-bed sandstone. The study provides a theoretical basis for the further investigation of the softening laws and mechanisms of other red layered soft rocks by temperature under turbulent conditions.
External Quality Assessment Evaluating the Ability of Dutch Clinical Microbiological Laboratories to Identify Candida auris
Authors: Buil Jochem B. , van der Lee Henrich A. L. , Curfs-Breuker Ilse , Verweij Paul E. , Meis Jacques F.
Published: October 2019

Background: Candida auris is a yeast that is causing nosocomial outbreaks in healthcare facilities around the world. There is a risk of the misidentification of C. auris with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)—when libraries are used that lack C. auris spectra, or when conventional biochemical methods are used. Methods: We conducted an external quality assessment to evaluate the ability of Dutch clinical microbiological laboratories to identify C. auris, and to raise awareness about the risk of misidentification. Results: 35/47 participating laboratories were able to identify C. auris correctly. Only 2/14 labs that potentially misidentified C. auris with their primary identification methods specified that they would perform additional tests to exclude C. auris when appropriate. 45/47 labs used MALDI-TOF MS systems to identify Candida species. Conclusions: There was a lack of awareness about the potential misidentification of C. auris in many labs that used MALDI-TOF MS with libraries that lacked C. auris spectra, and labs that used Vitek 2. However, as the currently available MALDI-TOF MS libraries in The Netherlands contain several C. auris spectra, we expect that currently almost all participating laboratories are able to identify C. auris correctly, as 45/47 participating laboratories use MALDI-TOF MS as their primary yeast identification method.
Trueness of the Inner Surface of Monolithic Crowns Fabricated by Milling of a Fully Sintered (Y, Nb)-TZP Block in Chairside CAD–CAM System for Single-visit Dentistry
Authors: Cho Jun-Ho , Yoon Hyung-In , Han Jung-Suk , Kim Dae-Joon
Published: October 2019

A single-visit zirconia restoration can be easily achieved if direct milling of a fully sintered zirconia block can be performed without much effort. However, no studies have yet been reported regarding the evaluation of the trueness of crown fabricated from chairside-milling of a fully sintered zirconia block in the chairside computer-aided design and computer-aided manufacturing (CAD–CAM) system for single-visit dentistry. This in vitro study aimed to evaluate the trueness of crowns fabricated by milling a fully sintered zirconia block in the chairside CAD–CAM system and investigate the clinical implications for single-visit chairside restoration. Crowns were fabricated either by chairside-milling a fully sintered block of niobium oxide containing yttria-stabilized tetragonal zirconia polycrystals ((Y, Nb)-TZP) without the sintering process (n = 12) in a chairside single-visit dentistry system (Chairside group) or by laboratory-milling a partially sintered 3 mol% block of yttria-stabilized tetragonal zirconia polycrystals (3Y-TZP) followed by the sintering process (n = 12) in a conventional laboratory system (Labside group). Crown fabrication time, milling tool diameter and the trueness of each crown were evaluated. All trueness values of both groups were within the clinically acceptable range, although a significant difference between the Chairside (43.0 ± 3.67 μm) and Labside groups (37.4 ± 2.41 μm) was observed (P < 0.05). Mean fabrication time was 0.52 h and 1.42 h for Chairside and Labside groups, respectively. A decrease in the tool diameter was observed for the Chairside group.
The Preparation of FeCo/ZnO Composites and Enhancement of Microwave Absorbing Property by Two-Step Method
Authors: Gao Ka , Zhao Junliang , Bai Zhongyi , Song Wenzheng , Zhang Rui
Published: October 2019

In this paper, the flower-like FeCo/ZnO composites were successfully firstly prepared by a two-step method, and their microstructures and microwave absorbing properties were characterized. The results show that with an increase of temperature, the content of ZnO loaded on a FeCo/ZnO composite surface was increased. The optimal reflection loss (RL) value can be reached around −53.81 dB at 9.8 GHz, which is obviously superior to results of previous studies and reports, and its effective bandwidth (RL < −10 dB) is 3.8 GHz in the frequency range of 8.7–11.8 GHz with a matching thickness of 1.9 mm. We considered that a large number of lamellar and rod-like ZnO loaded on nano-FeCo single-phase solid solution by two-step method can significantly improve the electromagnetic wave absorption properties.
Numerical Modelling of the Ultrasonic Treatment of Aluminium Melts: An Overview of Recent Advances
Authors: Lebon Bruno , Tzanakis Iakovos , Pericleous Koulis , Eskin Dmitry
Published: October 2019

The prediction of the acoustic pressure field and associated streaming is of paramount importance to ultrasonic melt processing. Hence, the last decade has witnessed the emergence of various numerical models for predicting acoustic pressures and velocity fields in liquid metals subject to ultrasonic excitation at large amplitudes. This paper summarizes recent research, arguably the state of the art, and suggests best practice guidelines in acoustic cavitation modelling as applied to aluminium melts. We also present the remaining challenges that are to be addressed to pave the way for a reliable and complete working numerical package that can assist in scaling up this promising technology.
Effect of Silica Fume in Concrete on Mechanical Properties and Dynamic Behaviors under Impact Loading
Authors: Zhao Shijun , Zhang Qing
Published: October 2019

The effect of silica fume (SF) in concrete on mechanical properties and dynamic behaviors was experimentally studied by split Hopkinson pressure bar (SHPB) device with pulse shaping technique. Three series of concrete with 0, 12%, and 16% SF as a cement replacement by weight were produced firstly. Then the experimental procedure for dynamic tests of concrete specimens with SF under a high loading rate was presented. Considering the mechanical performance and behaviors of the concrete mixtures, those tests were conducted under five different impact velocities. The experimental results clearly show concrete with different levels of SF is a strain-rate sensitive material. The tensile strength under impact loading of the tested specimens was generally improved with the increasing content of SF levels in concrete. Additionally, the tensile strength under impact loading of the concrete enhances with the increase of the strain rates. Finally, failure modes, dynamic tensile strength, dynamic increase factor (DIF), and critical strain are discussed and analyzed. These investigations are useful to improve the understanding of the effect of SF in concrete and guide the design of concrete structures.
Improvement in Fatigue Behavior of Dental Implant Fixtures by Changing Internal Connection Design: An In Vitro Pilot Study
Authors: Choi Nak-Hyun , Yoon Hyung-In , Kim Tae-Hyung , Park Eun-Jin
Published: October 2019

(1) Background: The stability of the dental implant–abutment complex is necessary to minimize mechanical complications. The purpose of this study was to compare the behaviors of two internal connection type fixtures, manufactured by the same company, with different connection designs. (2) Methods: 15 implant–abutment complexes were prepared for each group of Osseospeed® TX (TX) and Osseospeed® EV (EV): 3 for single-load fracture tests and 12 for cyclic-loaded fatigue tests (nominal peak values as 80%, 60%, 50%, and 40% of the maximum breaking load) according to international standards (UNI EN ISO 14801:2013). They were assessed with micro-computed tomography (CT), and failure modes were analyzed by scanning electron microscope (SEM) images. (3) Results: The maximum breaking load [TX: 711 ± 36 N (95% CI; 670–752), EV: 791 ± 58 N (95% CI; 725–857)] and fatigue limit (TX: 285 N, EV: 316 N) were higher in EV than those in TX. There was no statistical difference in the fracture areas (P > 0.99). All specimens with 40% nominal peak value survived 5 × 106 cycles, while 50% specimens failed before 105 cycles. (4) Conclusions: EV has improved mechanical properties compared with TX. A loading regimen with a nominal peak value between 40% and 50% is ideal for future tests of implant cyclic loading.
Cutting Performance Evaluation of the Coated Tools in High-Speed Milling of AISI 4340 Steel
Authors: Li Yuan , Zheng Guangming , Cheng Xiang , Yang Xianhai , Xu Rufeng , Zhang Huaqiang
Published: October 2019

The cutting performance of cutting tools in high-speed machining (HSM) is an important factor restricting the machined surface integrity of the workpiece. The HSM of AISI 4340 is carried out by using coated tools with TiN/TiCN/TiAlN multi-coating, TiAlN + TiN coating, TiCN + NbC coating, and AlTiN coating, respectively. The cutting performance evaluation of the coated tools is revealed by the chip morphology, cutting force, cutting temperature, and tool wear. The results show that the serration and shear slip of the chips become more clear with the cutting speed. The lower cutting force and cutting temperature are achieved by the TiN/TiCN/TiAlN multi-coated tool. The flank wear was the dominant wear form in the milling process of AISI 4340. The dominant wear mechanisms of the coated tools include the crater wear, coating chipping, adhesion, abrasion, and diffusion. In general, a TiN/TiCN/TiAlN multi-coated tool is the most suitable tool for high-speed milling of AISI 4340, due to the lower cutting force, the lower cutting temperature, and the high resistance of the element diffusion.
Stability Results for a Coupled System of Impulsive Fractional Differential Equations
Authors: Zada Akbar , Fatima Shaheen , Ali Zeeshan , Xu Jiafa , Cui Yujun
Published: October 2019

In this paper, we establish sufficient conditions for the existence, uniqueness and Ulam–Hyers stability of the solutions of a coupled system of nonlinear fractional impulsive differential equations. The existence and uniqueness results are carried out via Banach contraction principle and Schauder’s fixed point theorem. The main theoretical results are well illustrated with the help of an example.
Association of Higher Advanced Oxidation Protein Products (AOPPs) Levels in Patients with Diabetic and Hypertensive Nephropathy
Authors: Conti Giovanni , Caccamo Daniela , Siligato Rossella , Gembillo Guido , Satta Ersilia , Pazzano Dario , Carucci Nicolina , Carella Antonio , Del Campo Giuliana , Salvo Antonino , Santoro Domenico
Published: October 2019

Background and Objectives: Diabetes mellitus (DM) and hypertension (HT) are characterized by cell damage caused by inflammatory and metabolic mechanisms induced by alteration in reduction-oxidative status. Serum advanced oxidation protein products (AOPP) are new markers of protein damage induced by oxidative stress. We evaluated serum levels of AOPP in a cohort of patients with DM and HT, with or without renal complications, compared with a control healthy population. Materials and Methods: The study group comprised of 62 patients with type 2 DM and 56 with HT. The 62 patients affected by DM were further distinguished in 24 subjects without renal impairment, 18 with diabetic nephropathy (DN), 20 with chronic kidney disease (CKD) stage 2–3 secondary to DN. The subgroup of 56 patients with primary HT comprised 26 subjects without renal complications and 30 with CKD (stage 2–3) secondary to HT. Thirty healthy controls, matched for age and sex, were recruited among blood donors. Results: Increased AOPP levels were found in DM patients compared with healthy subjects, although not significantly. This index was higher and more significant in patients with DN and CKD secondary to DN than in DM patients without nephropathy (p < 0.05) or controls (p < 0.0001). Patients with HT and with kidney impairment secondary to HT also had significantly higher AOPP serum levels than controls (p < 0.01 and p < 0.0001, respectively). There were no significant differences in mean AOPP levels among DM and HT patients. Conclusion: Our study showed that oxidative stress was higher in diabetic or hypertensive subjects than in healthy controls and, in particular, it appeared to be more severe in patients with renal complications. We suggest that the assessment of AOPP in diabetic and hypertensive patients may be important to predict the onset of renal failure and to open a new perspective on the adoption of antioxidant molecules to prevent CKD in those settings.
Association between Itch and Cancer in 3836 Pediatric Pruritus Patients at a Tertiary Care Center
Authors: Belzberg Micah , Larson Valerie A. , Khanna Raveena , Williams Kyle A. , Semenov Yevgeniy , Ständer Sonja , Grossberg Anna L. , Kwatra Shawn G.
Published: October 2019

Background: Pruritus is a well-recognized paraneoplastic phenomenon. Previous studies have examined the association of itch with a variety of malignancies in adults. However, no large study has examined this association in a pediatric population. Methods: A retrospective study was conducted of patients age 18 or less seen at Johns Hopkins Health System between 2012 and 2019. Results: A pediatric hospital population of 1,042,976 patients was reviewed. Pruritus was observed in 3836 pediatric patients of whom 130 also had cancer. Pediatric patients with pruritus were significantly more likely to have concomitant malignancy compared to pediatric patients without pruritus (OR 12.84; 95% CI 10.73–15.35, p < 0.001). Malignancies most strongly associated with pruritus included neoplasms of the blood (OR 14.38; 95% CI 11.30–18.29, p < 0.001), bone (OR 29.02, 95% CI 18.28–46.06, p < 0.001) and skin (OR 22.76, 95% CI 9.14–56.72, p < 0.001. Conclusions: Pruritus is significantly associated with malignancy in the pediatric hospital population. Clinicians should also be aware of the high burden of itch in pediatric malignancies and the variation in pruritus across malignancies.
Examination of Formability Properties of 6063 Alloy Extruded Profiles for the Automotive Industry
Authors: Vazdirvanidis Athanasios , Pressas Ioannis , Papadopoulou Sofia , Toulfatzis Anagnostis , Rikos Andreas , Katsivarda Marianna , Symeonidis Grigoris , Pantazopoulos George
Published: October 2019

Bendability is a crucial property of automotive parts, which describes the ability of extruded profiles to be formed to shape, without the appearance of discontinuities that will have an adverse effect on the mechanical properties and their energy absorption capacity (crashworthiness). Anisotropic behavior exhibited by Al alloy extrusions has been documented due to the nature of the hot working process. In the present article an attempt is made in order to determine the main factors influencing bending properties in longitudinal and transverse to extrusion direction, which are also related with anisotropic behavior. Mechanical testing, microscopic examination and finite element analysis were applied in the frame of the current investigation. The findings were indicative that grain morphology and orientation (texture), especially on the surface zone of the extruded profiles, as well as morphology and distribution of the constituent particles are strongly related with formability.
A Silicocarbonatitic Melt and Spinel-Bearing Dunite of Crustal Origin at the Parker Phlogopite Mine, Notre-Dame-du-Laus, Quebec, Canada
Authors: Martin Robert F. , Schumann Dirk
Published: October 2019

The Parker phlogopite mine, located near Notre-Dame-du-Laus, Quebec, 74 km north of Ottawa, is well known among mineral collectors for its centimetric euhedral crystals of black spinel. Among the dozens of phlogopite mines active in the early 1900s in the Mont-Laurier–Bancroft corridor in the Central Metasedimentary Belt of the Grenville Province, the Parker mine is exceptional because of the association of forsterite + spinel with phlogopite. Euhedral crystals of these minerals are found “frozen” in a carbonate matrix. The carbonate dike and segregations are associated with spinel-rich dunite that contains accessory diopside, phlogopite, and pargasite, as well as ilmenite and apatite. The interstitial melt crystallized to calcite + dolomite. Hematite appeared as flakes in the melt owing to net loss of hydrogen, and the spinel underwent oxidation-induced exsolution. Our spinel crystal entrapped a domain of carbonate during growth. It also entrapped globules of boundary-layer melt that crystallized to a carbonate + sulfate + phosphate + silicate + oxide assemblage. Such globules, where present in the cumulate, are more pristine than in the coarse crystal of spinel, i.e., less affected by a hydrothermal overprint. We contend that the carbonate melt ultimately formed by the hydrous melting of marble, as supported by oxygen-isotope data on all major minerals. Melting occurred 1140 million years ago, at a time of tectonic relaxation following the Shawinigan compressive stresses.
Structure of Langmuir Monolayers of Perfluorinated Fatty Acids: Evidence of a New 2D Smectic C Phase
Authors: Fontaine Philippe , Filipe Eduardo J. M. , Fauré Marie-Claude , Rego Tomas , Taßler Stephanie , Alves Ana Carolina , Silva Gonçalo M. C. , Morgado Pedro , Goldmann Michel
Published: October 2019

Due to the characteristic chain rigidity and weak intermolecular interactions of perfluorinated substances, the phase diagram of Langmuir monolayer formed by perfluorinated molecules has been interpreted so far as displaying only two phases, a 2D gas (G) and a liquid condensed (LC). However, in this work, we presented Grazing Incidence X-ray Diffraction measurements, which exhibit two diffraction peaks on the transition plateau: One is the signature of the hexagonal structure of the LC phase, the second one is associated to the low-density fluid phase and is thus more ordered than expected for a 2D gas or a typical fluid phase. Atomistic molecular dynamics simulations, performed on the transition plateau, revealed the existence of clusters in which domains of vertical molecules organized in a hexagonal lattice coexist with domains of parallel lines formed by tilted molecules, a new structure that could be described as a “2D smectic C” phase. Moreover, the diffraction spectrum calculated from the simulation trajectories compared favorably with the experimental spectra, fully validating the simulations and the proposed interpretation. The results were also in agreement with the thermodynamic analysis of the fluid phase and X-ray Reflectivity experiments performed before and after the transition between these two phases.
Broccoli or Sulforaphane: Is It the Source or Dose That Matters?
Authors: Yagishita Yoko , Fahey Jed W. , Dinkova-Kostova Albena T. , Kensler Thomas W.
Published: October 2019

There is robust epidemiological evidence for the beneficial effects of broccoli consumption on health, many of them clearly mediated by the isothiocyanate sulforaphane. Present in the plant as its precursor, glucoraphanin, sulforaphane is formed through the actions of myrosinase, a β-thioglucosidase present in either the plant tissue or the mammalian microbiome. Since first isolated from broccoli and demonstrated to have cancer chemoprotective properties in rats in the early 1990s, over 3000 publications have described its efficacy in rodent disease models, underlying mechanisms of action or, to date, over 50 clinical trials examining pharmacokinetics, pharmacodynamics and disease mitigation. This review evaluates the current state of knowledge regarding the relationships between formulation (e.g., plants, sprouts, beverages, supplements), bioavailability and efficacy, and the doses of glucoraphanin and/or sulforaphane that have been used in pre-clinical and clinical studies. We pay special attention to the challenges for better integration of animal model and clinical studies, particularly with regard to selection of dose and route of administration. More effort is required to elucidate underlying mechanisms of action and to develop and validate biomarkers of pharmacodynamic action in humans. A sobering lesson is that changes in approach will be required to implement a public health paradigm for dispensing benefit across all spectrums of the global population.
Synthesis of a Di-Mycoloyl Tri-Arabinofuranosyl Glycerol Fragment of the Mycobacterial Cell Wall, Based on Synthetic Mycolic Acids
Authors: Ali Omar T. , Mohammed Mohsin O. , Al Dulayymi Juma’a R. , Baird Mark S.
Published: October 2019

Fragments of mycobacterial cell walls such as arabinoglycerol mycolate and dimycoloyl diarabinoglycerol, comprising complex mixtures of mycolic acids, have immunostimulatory and antigenic properties. A related di-mycoloyl tri-arabinofuranosyl glycerol fragment has been isolated from cell wall hydrolysates. An effective stereoselective synthesis of tri-arabinofuranosyl glycerol, followed by coupling with stereochemically defined mycolic acids of different structural classes, to provide unique di-mycoloyl tri-arabinofuranosyl glycerols is now described.