bluuebunny/crossref_metadata_2025 huggingface dataset:
Перспективи і сценарії розвитку НАТО в глобальній системі міжнародних відносин
Authors: Голопатюк Л.
Published: June 2021

На тлі подій останнього десятиліття в царині безпекової ситуації вже став очевидним сценарій, який передбачає повернення НАТО до більш рішучих кроків у вирішенні питань колективної оборони та безпеки.

Відмова ж від такої перспективи, з урахуванням вже розгорнутих підрозділів збройних сил країн-членів Альянсу на території країн Східної Європи, перш за все, Польщі, Румунії та країнах Балтії, призведе до уявлення (якщо не впевненості) в неповноцінності тих заходів, які вживаються, та ствердження, що НАТО не готове до дій в глобальному плані.

Для України в її прагненні щодо набуття членства в Альянсі, дуже важливим є з високою долею вірогідності передбачити воєнно-політичний розвиток НАТО та, з урахуванням таких прогнозів, сформулювати напрями своєї політики, включаючи питання військової співпраці по відношенню до Організації Північноатлантичного Договору.

Дана стаття є продовження дискусії, яка запропонована авторами публікації “Майбутнє Північноатлантичного Альянсу в умовах невизначеності та складного геополітичного оточення” стосовно майбутнього місця НАТО в глобальній системі міжнародних відносин, де проведений аналіз бачення розвитку Організації Північноатлантичного договору на довгострокову перспективу. І фактично є спробою спрогнозувати напрями політики Альянсу, міжнародну ситуацію, та воєнно-політичну обстановку в цілому, відповідно до запропонованих сценаріїв. Вірогідність та, у підсумку, успішність реалізації одного із зазначених сценаріїв, залежить від низки стратегічних факторів.

В статті проведено аналіз найбільш вірогідних сценаріїв розвитку НАТО, а саме: “євроцентричне НАТО”, “глобальне НАТО”, “гнучке НАТО”. Проаналізовано кожний з наведених сценаріїв, визначені всі позитивні і негативні його сторони та наслідки. Також важливим є значення кожного із сценаріїв на політику України. Найбільш сприятливим для більш ґрунтовного співробітництва та надання Україні необхідної підтримки виділяється сценарій “гнучке НАТО”.
Питання підвищення ефективності управління мобілізаційною підготовкою оборонного сектору національної економіки України
Authors: Гріненко О.
Published: June 2021

Система державного управління мобілізаційною підготовкою є складовою частиною загальної системи управління державою, яка спрямовує свою діяльність в тому числі на мобілізаційну готовність оборонного сектору національної економіки України, ресурсне забезпечення заходів, що проводяться.

Визначені шляхи підвищення ефективності управління мобілізаційною підготовкою оборонного сектору національної економіки України та ролі  центральних органів виконавчої влади, органів державної влади, інших державних органів, відповідальних за впровадження заходів мобілізаційної підготовки в держави.

Мобілізаційну підготовку оборонного сектору національної економіки, як це і передбачає діюча нормативно-правова база держави,доцільно розглядати  за чотирма основними напрямами, а саме:

мобілізаційну підготовку до функціонування системи управління державою в особливий період;

мобілізаційну підготовку до мобілізаційного розгортання Збройних Сил України, інших військових формувань, Оперативно-рятувальної служби цивільного захисту;

мобілізаційну підготовку до функціонування національної економіки в особливий період;

мобілізаційну підготовку до функціонування системи забезпечення життєдіяльності населення в особливий період.

Вирішення питань мобілізаційної підготовки національної економіки  у складі діючої економіки ускладняється незавершеністю галузевого структурування за багатьма напрямами. Тому в існуючий структурі національної економіки мобілізаційну підготовку підприємств повинен організовувати центральний орган виконавчої влади в інтересах якого буде працювати зазначене підприємство.

Пропонується створити в складі зазначених центральних органів виконавчої влади потужні виконавчі структури.
Research on Initial Behavior of Screwed Lap Connections of Thin Plates – Experimental Tests and FEM Analysis
Authors: Gryniewicz Marcin
Published: June 2021

The paper continues the past research on the screwed thin-plate single-lap connections undertaken by the author. This re-search was focused on stressed skin diaphragm action, where it is essential to know values of connections behavior. Until now, initial stiffness is used for common engineering estimations. The main aim of this paper is to compare the experimental results of the shear tests conducted on the connections with the theoretical predictions of their initial behavior obtained from the FEM models. The experiments followed the Eurocode rules for design aided by testing and the European Convention for Constructional Steelwork (ECCS) recommendations. FEM parametric models are introduced as a consideration to use, taking into account screw threads and EPDM washers. In the technical literature, various techniques are described for the modelling of threaded connections. A modification to a known solution is applied in this analysis where a spiral thread is substituted by simple horizontal rings; this simplification allows use of symmetry in a static system, which is particularly desirable for large models such as those prepared in this paper. In contrast to the mentioned known solutions, the thread mod-el presented here has a triangular cross-section related to the geometry used in real screws. The numerical results correspond reasonably well to the test’s early stage measurements from the lab experiments and can be useful for initial stiffness examination (e.g. where no data is provided for a particular connector by a producer) or as a base to further research on the model.
Використання стратегічного маркетингового інструментарію в межах торговельного співробітництва
Authors: Птащенко О.В. , Куценко К.І.
Published: June 2021

У статті розглянуто стратегічний маркетинговий інструментарій, який використовують підприємства в межах здійснення торговельного співробітництва.

Визначено, міжнародний маркетинг є самостійною галуззю діяльності підприємства за умови виходу на зовнішні ринки, яка є системою планування, реалізації, контролю та аналізу заходів по впливу на багатонаціональне ринкове середовище і пристосування до її умов на підприємстві, яке здійснює свою діяльність більш ніж в одній країні. Представлено розгляд основного інструментарію міжнародного маркетингу.

Відтворювальна відкритість країни при використанні міжнародного маркетингу залежить від наявності в державі природних багатств – енергетичних ресурсів, сировини для промисловості, продовольства для населення. Рівень відтворювальної відкритості держави тим вищий, чим вищий рівень її техніко-економічного розвитку та чим менша величина її ВВП і забезпеченість власними природними ресурсами.

Тому за таких умов розвитку світової економіки залучення технологій розглядають як один з головних чинників зростання економіки як трансформаційних країн, так і розвинутих. Вивчення міжнародного ринку сучасних технологій, зокрема процесів еволюції, формування елементів та їх структури, є актуальним питанням сьогодення. Активна участь на міжнародному ринку й є запорукою доступу до передових знань, стимулювання внутрішньої інноваційної діяльності, підвищення продуктивності економічних процесів. З огляду на зазначене, можна стверджувати, що міжнародний маркетинг – це така система організації діяльності суб'єктів виробничої сфери, яка сприяє оптимізації функціонального поєднання виробничих цілей і поточних потреб різних груп споживачів, координації приватних інтересів у всіх сферах економічного життя – виробничої, посередницької, споживчої по відношенню – до глобального міжнародного ринку.
Становлення глобального економічного простору під впливом економіки знань
Authors: Птащенко О.В.
Published: June 2021

У статті розглянуто основні особливості глобального екномічного простору сьогодення.

Визначено, що процес формування глобального економічного простору пройшов декілька етапів: становлення (XIX ст.); боротьба національних капіталів, підтримуваних державою за панування у глобальній системі міжнародних економічних відносин, що складалася (перша половина XX ст.); розкол світу на дві системи, які функціонували на основі різних принципів і перебували у стані антагоністичної єдності (період холодної війни); перехід до етапу глобалізації ринкової економіки, створення єдиних економічних просторів, панування транснаціональних компаній у світовій економіці (кінець XX – початок XXI ст.).

Розглянуто, що темпи сучасного науково-технічного розвитку та залежність від ефективного використання науки та техніки провідними країнами та економічне зростання світової економіки зумовили необхідність консолідації зусиль та ресурсів різних країн для досягнення національних та глобальних науково-технічних цілей. Наукове, технологічне та інноваційне співробітництво держав, переваги міжнародної спеціалізації та співпраця в галузі досліджень та розробок є важливими для подальшого розвитку цивілізації. Сьогодні динаміка інтернаціоналізації та глобалізації, що є основою для ефективного розвитку всіх сфер суспільства, відображена в науково-технічній сфері.

Таким чином, визначено у роботі основні фактори впливу на глобальний економічний простір з урахування специфіки становлення економіки знань сьогодні.
THE IMPACT OF A PROFESSIONAL DEVELOPMENT PROGRAM ON IN-SERVICE TEACHERS’ TPACK: A STUDY FROM ESTONIA
Authors: Lehiste Piret
Published: June 2021

Recently there has been a considerable interest in the Technological Pedagogical Content Knowledge (TPACK) framework for effective technology integration (Koehler et al., 2013). Researchers have been measuring how effective their professional development efforts have been for the development of teachers’ TPACK (Graham et al., 2009; Guzey & Roehrig, 2009). So far research is based only on short-term courses, so longitudinal studies are needed to examine the TPACK development of teachers across time (Hoffer & Grandgenett, 2012; Koh & Sing, 2011).
A two-year in-service training program of educational technology (60 ECTS), based on the TPACK framework, was developed at Tallinn University Haapsalu College to support in-service teachers to effectively embed ICT into their classroom teaching. The first group of teachers (n=20) enrolled in the program in January 2014. This paper reports some preliminary findings of a longitudinal action research, the main aim of which is to evaluate the impact of the in-service training program of educational technology on teachers’ TPACK.
The key questions for this research were: how do teachers’ perceptions of their TPACK levels change after participating in the training program for a year; which of the seven knowledge domains of the TPACK framework develop more than others; what is the impact of different knowledge domains on teachers’ TPACK perceptions.
The TPACK questionnaire developed by Schmidt et al. (2009) was used to measure in-service teachers’ self-assessments of their TPACK during the first meeting of the training program and at the end of the first academic year.
The research results revealed increase in all the domains of the TPACK framework. There was significant development of the teachers’ TK and TPACK, but only limited growth in CK and PK. Strong positive correlations were found between TCK, TPK and TPACK in the post-course survey.
Key words: ICT, in-service teachers, professional development, TPACK.
Inhibitors of ADAM10 reduce Hodgkin lymphoma cell growth in 3D microenvironments and enhance brentuximab-vedotin effect
Authors: Pece Roberta , Tavella Sara , Costa Delfina , Varesano Serena , Camodeca Caterina , Cuffaro Doretta , Nuti Elisa , Rossello Armando , Alfano Massimo , D’Arrigo Cristina , Galante Denise , Ravetti Jean-Louis , Gobbi Marco , Tosetti Francesca , Poggi Alessandro , Zocchi Maria Raffaella
Published: June 2021

Shedding of ADAM10 substrates, like TNFa or CD30, can affect both anti-tumor immune response and antibody-drug-conjugate (ADC)-based immunotherapy. We have published two new ADAM10 inhibitors, LT4 and MN8 able to prevent such shedding in Hodgkin lymphoma (HL). Since tumor tissue architecture deeply influences the outcome of anti-cancer treatments, we set up a new threedimensional (3D) culture systems to verify whether ADAM10 inhibitors can contribute to, or enhance, the anti-lymphoma effects of the ADC brentuximab-vedotin (BtxVed). In order to recapitulate some aspects of lymphoma structure and architecture, we assembled two 3D culture models: mixed spheroids made of HL lymph node (LN) mesenchymal stromal cells (MSC) and Reed Sternberg/Hodgkin lymphoma cells (HL cells) or collagen scaffolds repopulated with LN-MSC and HL cells. In these 3D systems we found that: i) the ADAM10 inhibitors LT4 and MN8 reduce ATP content or glucose consumption, related to cell proliferation, increasing lactate dehydrogenase release as a cell damage hallmark; ii) these events are paralleled by mixed spheroids size reduction and inhibition of CD30 and TNFa shedding; iii) the effects observed can be reproduced in repopulated HL LN-derived matrix or collagen scaffolds; iv) ADAM10 inhibitors enhance the anti-lymphoma effect of the anti-CD30 ADC BtxVed both in conventional cultures and in repopulated scaffolds. Thus, we provide evidence for a direct and combined antilymphoma effect of ADAM10 inhibitors with BtxVed, leading to the improvement of ADC effects; this is documented in 3D models recapitulating features of the LN microenvironment, that can be proposed as a reliable tool for anti-lymphoma drug testing.
Pragmáticas, dóciles, cuestionadoras: la pregunta por la agencia en usuarias de TRHA
Authors: Johnson María Cecilia
Published: June 2021

Este trabajo explora las maneras en que las mujeres usuarias de tecnología reproductiva en Argentina construyen diversas formas de agencia a lo largo de sus itinerarios reproductivos. Las tecnologías reproductivas forman parte de los procesos de control y medicalización creciente sobre la reproducción y sexualidad, aunque paradójicamente este mismo dispositivo biomédico en presentado en nuestra sociedad como la posibilidad más simple para que las mujeres accedan a la maternidad.  Esta tensión que algunos sectores académicos advierten sobre la tecnología reproductiva se expresa en las experiencias de las usuarias en diferentes formas de agencia, las cuales amplifican la idea de agencia como resistencia a la biomedicina. Desde un enfoque biográfico se analizan las experiencias de las usuarias y se proponen diversas categorías a partir del trabajo de investigación para comprender la complejidad de la agencia reproductiva de las usuarias.
Comparative seasonal assessment on the quality of Black Bengal goat oocytes in view of in vitro embryo production
Authors: Sarkar Shikha , Islam Md Saiful , Kabir Md Enayet , Dadok Falguni , Zaber Md Abdullah Al , Abdulla Md Shefath
Published: June 2021

In vitro maturation (IVM) of oocytes provide an excellent opportunity for cheap and abundant embryos for carrying out animal improvement. With the aim for studying the quality of oocyte in different seasons of Black Bengal goat, both right and left ovaries were collected from the slaughter houses. For each of the specimens, gross parameters such as right, left, corpus luteum (CL)-present and corpus luteum (CL)-absent group were evaluated on the basis of weight (g), length (cm), width (cm), follicles aspirated and number and state of cumulus-oocyte-complexes (COCs), normal COCs and abnormal COCs, the effect of season on cumulus oocyte complexes (COCs) of goat oocytes. Our study revealed that the average number of follicles and oocytes recovery rate were higher in summer than in winter season and recovery rate of grade A and B oocytes were higher in summer than that of in winter and recovery rate of grade C and D were higher in winter than that of in summer. In the present study a significantly greater number of oocytes per ovary were recovered from ovaries without a corpus luteum than from ovaries with a corpus luteum. So the summer was the best season for recovery of COCs which have a worthy competence to be matured in vitro.

Asian Australas. J. Biosci. Biotechnol. 2020, 5 (3), 78-87
Postpartum Acute Kidney Injury - a Study at Faridpur Medical College Hospital
Authors: Mondal Swapan Kumar , Biswas Ananta Kumar , Joy Swapna Biswas , Islam MM Shahin Ul , Pal Bablu Kumar , Kar Shubharthi , Anisuzzaman Syed , Muktadir Syed Golam
Published: June 2021

Postpartum Acute Kidney Injury (AKI) is one of the serious complications of pregnancy and is associated with high mortality and morbidity. Although Postpartum AKI has decreased markedly in developed countries in the last 50 years, but it is a challenging health problem in pregnant women, specially in the developing countries like Bangladesh. Aim of this study was to determine the contributing factors and outcome of postpartum AKI. This observational study was conducted in the department of nephrology, Faridpur Medical College Hospital from January 2018 to December 2019. During that study period thirty two patients with postpartum AKI were admitted in the above mentioned department. Demographic, clinical and laboratory data of those patients were recorded. Mean age of patients was 25.3 ± 4.2 years, and the 72% patients underwent cesarean section. Oliguria was the main presentation (91%). Puerperal sepsis (38%), postpartum hemorrhage (22%), and Eclampsia (16%) were the common contributing factors. Hemodialysis was required for most of the patients (75%). Therefore early diagnosis, identification and treatment of cause, timely initiation of renal replacement therapy is mandatory.
Faridpur Med. Coll. J. 2020;15(2): 62-64
Glanzmann's Thrombasthenia: A rare platelet functional disorder
Authors: Kamruzzaman Muhammad , Hossain Mohammad Iqbal , Rahman Mohammad Ashiqur , Sharifunnesa -
Published: June 2021

Glanzmann's Thrombasthenia (GT) is a rare inherited autosomal recessive platelet functional disorder. Due to the deficiency of platelet function, it manifests as a bleeding disorder characterized by mucocutaneous hemorrhage of varying severity. It is difficult to diagnose as it closely mimics with others bleeding disorder, so it can be diagnosed after investigations & exclusion of others. Treatment is supportive care with platelet transfusion & proper counseling. With careful supportive care, GT has a very good prognosis. In this report, we describe a 13 years old female with Glanzmann Thrombasthenia.
Faridpur Med. Coll. J. 2020;15(2): 103-105
Tuberculosis of the Gallbladder: An uncommon Case Report
Authors: Biswas Swapan Kumar , Khan Saiful Islam , Hossain Muhammad Mofazzal
Published: June 2021

Isolated gall bladder tuberculosis (GBTB) is exceedingly rare even in an endemic region and is usually found as a GB mass in association with cholelithiasis. Confirmed preoperative diagnosis is very difficult, and most cases are diagnosed after cholecystectomy. We present a case of a 45-years-old woman who came with symptoms of chronic cholecystitis. Computed tomography scan revealed intraluminal gallbladder mass and cholelithiasis. The patient underwent open cholecystectomy and GBTB was diagnosed after histopathological examination. Histopathological examination should be done after all cholecystectomy operations.
Faridpur Med. Coll. J. 2020;15(2): 106-107
HUBUNGAN ANTARA KUALITAS LAYANAN DENGAN KEPUASAN KONSUMEN DI RUMAH CANTIK AMANIE KEBUN TEBENG KOTA BENGKULU
Authors: Cahyani Regita , Riskan Riskan , Jasma Suardi
Published: June 2021

This study was aimed to figure out if there is significant correlation between service quality and customers' satisfaction at Rumah Cantik Amanie in Kebun Tebeng of Bengkulu municipality. This was a quantitative study. Method used in this study was survey. Data collection was done by giving away questionnaire in the form of written statement to be filled in by the respondents based on the real situation. The respondents were 212 people, taken by purposive sampling technique. Data analysis method used were descriptive analysis and correlation analysis. Based on the analysis result and hipothesis examination it could be concluded that: (1) Amanie salon's service quality had been quality in the customers'  opinion proven by average score of 4.04. This  average score was in the range of 3.41 - 4.20 which was categorized to be "quality"; (2) Amanie salon's customers' satisfaction had already been satisfied category, with average answer of 4.09. This score was in the range of 3.41-4.20 which meant "satisfied"; (3) There was correlation between service quality and Amanie salon's customers' satisfaction with correlation coefficent of 0.977.  The capability of service quality variable in explaining the rise and fall of Amanie Salon's customers' satisfaction was still huge. It was 95.45%, while the rest 4.55% was affected by other factor which was not studied. Keywords : service quality, customers' satisfaction
Fatores condicionantes para a tendência de uso de tecnologias de informação e comunicação (TICs) no ensino superior em Moçambique
Authors: Samussone Lainesse Benjamim , Silveira Suely de Fátima Ramos , Brunozi Júnior Antônio Carlos , Alexandre Denyse Claudette Sebastião , Reis Anderson Oliveira
Published: June 2021

O uso crescente das TICs e a sua capacidade de transformar os processos educacionais tem sido assunto de destaque nas discussões de políticas de educação superior, despertando a atenção para a necessidade de debates acadêmicos que visam entender aspetos ligados a aceitação e uso das TICs nos processos de ensino. O presente estudo, recorre à Teoria Unificada de Aceitação e Uso da Tecnologia (UTAUT), para analisar os fatores que os docentes do ensino superior púbico, percebem como sendo determinantes e que influenciam a tendência de utilização de TICs nas IES em Manica, Moçambique.  Para a coleta de dados, foi desenvolvido um questionário online e enviado a 207 docentes das IES públicas da província de Manica, dos quais, 103 responderam. Os dados foram analisados usando a análise fatorial e a regressão logística binominal. Os resultados do estudo indicaram que os docentes percebem como fatores determinantes para a utilização TICS a expectativa de desempenho, as condições facilitadoras e a expectativa de esforço. Estes três fatores contribuem positivamente na tendência de uso das TICs.
A melhoria contínua em uma fábrica de pão de queijo de São Paulo: Um estudo de caso
Authors: Sousa Evaldo Ferreira de , Oliveira Cristina Corrêa de
Published: June 2021

O mercado está se tornando progressivamente mais competitivo, o que torna a continuidade de uma organização mais trabalhosa, pois para assegurar sua fatia de mercado ela deve se manter competitiva, com melhoria contínua no processo fabril e nos resultados. Este estudo desenvolveu um estudo do processo produtivo de uma pequena empresa produtora de pão de queijo, na cidade de São Paulo. Foi realizado um estudo de caso, iniciado com uma pesquisa sobre o processo produtivo da empresa, tendo como pressuposto um levantamento bibliográfico sobre o processo e a filosofia Kaizen, em conjunto com as ferramentas 3Ms e Matriz de GUT. Foram considerados os dados quantitativos e qualitativos que permitiram analisar os pontos de vulnerabilidade da empresa e necessidade de melhorias. Foram detectados pontos de melhoria no processo produtivo, especificamente no rearranjo físico, com movimentação desnecessária de materiais e pessoas, bem como de manutenção nos fornos.  A partir das ferramentas utilizadas, foi possível mapear o processo produtivo, encontrar os maiores problemas e trabalhar em parceria com os funcionários e o proprietário para implementar soluções otimizadas para a empresa com redução da movimentação e aumento da produção.
Os desafios do complexo da tristeza parasitária bovina – TPB
Authors: Costa Mariah Oliveira , Carvalho Monique Resende , Gomes Lianna Ghisi , Stocco Matias Bassinello , Spiller Paulo Roberto , Faria Eduardo Ferreira , Nogueira Elce Nunes Nogueira da Costa , Dall’Acqua Priscila Chediek , Paula Eric Mateus Nascimento de , Mendes Andresa de Cássia Martini
Published: June 2021

Este artigo apresenta os principais desafios do complexo da Tristeza Parasitária Bovina (TPB), a partir de revisão literária sobre o tema. A TPB é um complexo de enfermidades causadas pelos protozoários Babesia bovis e Babesia bigemina combinados com a bactéria Anaplasma marginale, os quais são transmitidos pelo vetor Rhipicephalus (Boophilus) microplus (carrapato-do-boi) ao seu hospedeiro bovino, impactando negativamente a saúde do animal e acarretando milhões de prejuízos à economia rural, seja no combate e controle do parasito, ou em gastos com medicamentos, de acordo com dados do Ministério da Agricultura, Pecuária e Abastecimento (MAPA), e do IBGE. Os principais métodos de controle e tratamento às doenças causadas por B. bovis, B. bigemina e A. marginale, atualmente, se resumem no combate ao vetor com aplicação de produtos químicos, como os acaricidas, aos animais contaminados. Entretanto, os desafios desse método residem na prática de aplicação em sequência e com frequência desses produtos químicos na população bovina, o que provoca, geralmente, a configuração de carrapatos multirresistentes aos agentes químicos. Diversos estudos têm mostrado pequenos resultados com tratamentos homeopáticos e rotação de pastagem, tratamentos alternativos para o combate ao parasito Rhipicephalus microplus. Em 2020, o MAPA lançou obra orientativa com a metodologia Tratamento Bovino Seletivo (TBS), cujo objetivo é acompanhar caso a caso a população bovina contaminada para se fazer o processo seletivo dos animais, de modo que os animais doentes são tratados. Para garantir êxito, oferece-se o Programa de Controle Seletivo do Carrapato Bovino (PCSCB), criado pela Universidade Federal do Paraná.
Bölgesel ve Tüm Vücut Foam Roller Uygulamalarının 1 Tekrar Maksimal Kuvvet Ölçümüne Etkisi
Authors: Edis Çağlar , Vurgun Hikmet
Published: June 2021

Bu çalışmanın amacı sadece bölgesel olarak quadriceps-hamstring (BFR) ve tüm vücuda uygulanan (calf, hamstring, quadriceps, adductor ve latisimusdorsi) foam roller (TFR) uygulamalarının quadriceps ve hamstring (H:Q) 1 Tekrar maksimal (1TM) kuvvet üzerine etkilerini araştırmaktır. Çalışmaya Türkiye bölgesel amatör Liginde futbol oynayan toplam 14 gönüllü sporcu katılım gösterdi (Yaş: 20.8±3.9, Boy: 1.78±.054, Kg: 64.5±9.6, Vücut kütle indeksi: 20.2±2.5). Sporculara ilk aşamada BFR uygulamaları 2x30 saniye olacak şekilde H:Q kas gruplarına uygulatıldı ve ardından 1TM kuvvet ölçümleri tükeninceye kadar uygulatıldı. 3 gün sonra FR uygulaması tüm vücuda 2x30 saniye uygulandı ve ardından sporcular 1TM kuvvet testini tükeninceye kadar gerçekleştirildi. Çalışmanın istatistiksel analizlerinde verilerin normal dağılım uygunluğuna göre paired sample t-testi ve wilcoxon sıralı işaretler testi uygulanıp daha sonrasında etki büyüklüğü hesaplandı. Sonuç olarak; BFR uygulamalarına göre TFR uygulamalarının H:Q 1TM kuvveti üzerine olumlu etkisi ortaya çıkarttığı görülmüştür (p <0.05). Bu araştırmanın sonuçları antrenörlere sporcularının 1TM kuvvet ölçümleri öncesinde tüm vücuda uygulayacakları FR uygulamaları sonrasında maksimal kuvvet sonuçlarından daha fazla verim alabilmeleri konusunda bilgiler verebilmektedir.
Altyapı Sporcularının Antrenör-Sporcu İlişkilerinin Ahlaki Karar Alma Tutumlarına Etkisinin Belirlenmesi
Authors: Canlı Tülay , Canlı Umut , Taşkın Cüneyt
Published: June 2021

Bu araştırma altyapı sporcularının bazı değişkenler ile antrenör-sporcu ilişkisi düzeylerinin sporcuların ahlaki karar alma tutumlarına etkisinin ortaya konulmasını amacı ile yapılmıştır. Araştırmaya 109 erkek sporcu, 131 kadın sporcu (yaş = 13,5±1,71) olmak toplam 240 sporcu gönüllü olarak katılmıştır. Araştırmada Antrenör-Sporcu İlişkisi Envanteri ve Altyapı Sporlarında Ahlaki Karar Alma Tutumları ölçekleri veri toplama aracı olarak kullanılmıştır. Verilerin istatistiksel analizinde; betimsel istatistik, doğrulayıcı faktör analizi, Cronbach alfa ve hiyerarşik regresyon analizi kullanılmıştır. Alt yapı sporcularının yaş, spor yaşı, antrenör ile çalıştığı sürenin özgün katkısının anlamlı olduğu ve hileyi benimsemek alt boyutunu (R=0,278; R2=0,077; Düzeltilmiş R2=0,065; F(3-236)= 6,582; p<0,05) ve yarışma severlik alt boyutunu (R=0,207; R2=0,043; Düzeltilmiş R2=0,031; F(3-236)= 3,538; p<0,05) etkilediği belirlenmiştir. Antrenör-sporcu ilişkisinin özgün katkısının da anlamlı olduğu hem hileyi benimsemek hem de yarışma severlik alt boyutunu etkilediği belirlenmiştir (p<0.05). Antrenör-sporcu ilişkisinin ise özgün katkısının model içerisinde çok yüksek düzeyde anlamlı olduğu tespit edilmiştir. (p<0,05). Sonuç olarak; yaş ve spor yaş düzeyinin artması ile beraber zayıf düzeyde olsa hileyi benimseme düzeylerinin azaldığı belirlenmiştir. Antrenör ve sporcu arasında karşılıklı saygı, güven ve değer vermeyi ifade eden yakınlık alt boyutu ile ahlaki karar almada olumlu tutum ve davranışı temsil eden adilce kazanmak alt boyutu arasında pozitif doğrusal ilişkinin olduğu tespit edilmiştir.
Gerard Keller, 1829-1899
Authors: van de Schoor Rob
Published: June 2021

Gerard Keller groeide op in dezelfde omstandigheden waarin veel van de personages in zijn verhalen en romans leven, en die bekend staan als fatsoenlijke armoede. Juist in Den Haag, de deftige residentie, waar veel ambtenaren, zoals Gerards vader, lange dagen moesten werken voor het kleinste traktement, probeerden tallozen een stand op te houden die ze niet konden betalen. In Gouda geboren, op 13 februari 1829, verhuisde de twee jaar oude Gerard met zijn ouders en broertjes en zusjes naar Den Haag, waar hij zou schoolgaan en van 1844 tot 1846 zelfs het gymnasium bezocht. Hij verliet die school voortijdig, om in Delft te gaan studeren, maar toen er in 1848 kwade tijden aanbraken, moest hij zijn studie afbreken en naar huis terugkeren. Hij probeerde een tijdlang zonder succes zich te bekwamen voor verschillende beroepen, tot hij in 1849 werd aangesteld als stenograaf in de Tweede Kamer.
Surgical Application of Human Amniotic Membrane and Amnion-Chorion Membrane in the Oral Cavity and Efficacy Evaluation: Corollary With Ophthalmological and Wound Healing Experiences
Authors: Odet Stéphane , Louvrier Aurélien , Meyer Christophe , Nicolas Francisco J. , Hofman Nicola , Chatelain Brice , Mauprivez Cédric , Laurence Sébastien , Kerdjoudj Halima , Zwetyenga Narcisse , Fricain Jean-Christophe , Lafarge Xavier , Pouthier Fabienne , Marchetti Philippe , Gauthier Anne-Sophie , Fenelon Mathilde , Gindraux Florelle
Published: June 2021

Due to its intrinsic properties, there has been growing interest in human amniotic membrane (hAM) in recent years particularly for the treatment of ocular surface disorders and for wound healing. Herein, we investigate the potential use of hAM and amnion-chorion membrane (ACM) in oral surgery. Based on our analysis of the literature, it appears that their applications are very poorly defined. There are two options: implantation or use as a cover material graft. The oral cavity is submitted to various mechanical and biological stimulations that impair membrane stability and maintenance. Thus, some devices have been combined with the graft to secure its positioning and protect it in this location. This current opinion paper addresses in detail suitable procedures for hAM and ACM utilization in soft and hard tissue reconstruction in the oral cavity. We address their implantation and/or use as a covering, storage format, application side, size and number, multilayer use or folding, suture or use of additional protective covers, re-application and resorption/fate. We gathered evidence on pre- and post-surgical care and evaluation tools. Finally, we integrated ophthalmological and wound healing practices into the collected information. This review aims to help practitioners and researchers better understand the application of hAM and ACM in the oral cavity, a place less easily accessible than ocular or cutaneous surfaces. Additionally, it could be a useful reference in the generation of new ideas for the development of innovative protective covering, suturing or handling devices in this specific indication. Finally, this overview could be considered as a position paper to guide investigators to fulfill all the identified criteria in the future.
Genetic Characterization of Cancer of Unknown Primary Using Liquid Biopsy Approaches
Authors: Laprovitera Noemi , Salamon Irene , Gelsomino Francesco , Porcellini Elisa , Riefolo Mattia , Garonzi Marianna , Tononi Paola , Valente Sabrina , Sabbioni Silvia , Fontana Francesca , Manaresi Nicolò , D’Errico Antonia , Pantaleo Maria A. , Ardizzoni Andrea , Ferracin Manuela
Published: June 2021

Cancers of unknown primary (CUPs) comprise a heterogeneous group of rare metastatic tumors whose primary site cannot be identified after extensive clinical–pathological investigations. CUP patients are generally treated with empirical chemotherapy and have dismal prognosis. As recently reported, CUP genome presents potentially druggable alterations for which targeted therapies could be proposed. The paucity of tumor tissue, as well as the difficult DNA testing and the lack of dedicated panels for target gene sequencing are further relevant limitations. Here, we propose that circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) could be used to identify actionable mutations in CUP patients. Blood was longitudinally collected from two CUP patients. CTCs were isolated with CELLSEARCH® and DEPArrayTM NxT and Parsortix systems, immunophenotypically characterized and used for single-cell genomic characterization with Ampli1TM kits. Circulating cell-free DNA (ccfDNA), purified from plasma at different time points, was tested for tumor mutations with a CUP-dedicated, 92-gene custom panel using SureSelect Target Enrichment technology. In parallel, FFPE tumor tissue was analyzed with three different assays: FoundationOne CDx assay, DEPArray LibPrep and OncoSeek Panel, and the SureSelect custom panel. These approaches identified the same mutations, when the gene was covered by the panel, with the exception of an insertion in APC gene. which was detected by OncoSeek and SureSelect panels but not FoundationOne. FGFR2 and CCNE1 gene amplifications were detected in single CTCs, tumor tissue, and ccfDNAs in one patient. A somatic variant in ARID1A gene (p.R1276∗) was detected in the tumor tissue and ccfDNAs. The alterations were validated by Droplet Digital PCR in all ccfDNA samples collected during tumor evolution. CTCs from a second patient presented a pattern of recurrent amplifications in ASPM and SEPT9 genes and loss of FANCC. The 92-gene custom panel identified 16 non-synonymous somatic alterations in ccfDNA, including a deletion (I1485Rfs∗19) and a somatic mutation (p. A1487V) in ARID1A gene and a point mutation in FGFR2 gene (p.G384R). Our results support the feasibility of non-invasive liquid biopsy testing in CUP cases, either using ctDNA or CTCs, to identify CUP genetic alterations with broad NGS panels covering the most frequently mutated genes.
A Living Cell Repository of the Cranio-/Orofacial Region to Advance Research and Promote Personalized Medicine
Authors: Parisi Ludovica , Knapp Patrick O. , Girousi Eleftheria , Rihs Silvia , La Scala Giorgio C. , Schnyder Isabelle , Stähli Alexandra , Sculean Anton , Bosshardt Dieter D. , Katsaros Christos , Degen Martin
Published: June 2021

The prevalence of congenital anomalies in newborns is estimated to be as high as 6%, many of which involving the cranio-/orofacial region. Such malformations, including several syndromes, are usually identified prenatally, at birth, or rarely later in life. The lack of clinically relevant human cell models of these often very rare conditions, the societal pressure to avoid the use of animal models and the fact that the biological mechanisms between rodents and human are not necessarily identical, makes studying cranio-/orofacial anomalies challenging. To overcome these limitations, we are developing a living cell repository of healthy and diseased cells derived from the cranio-/orofacial region. Ultimately, we aim to make patient-derived cells, which retain the molecular and genetic characteristics of the original anomaly or disease in vitro, available for the scientific community. We report our efforts in establishing a human living cell bank derived from the cranio-/orofacial region of otherwise discarded tissue samples, detail our strategy, processes and quality checks. Such specific cell models have a great potential for discovery and translational research and might lead to a better understanding and management of craniofacial anomalies for the benefit of all affected individuals.
TKI-Resistant Renal Cancer Secretes Low-Level Exosomal miR-549a to Induce Vascular Permeability and Angiogenesis to Promote Tumor Metastasis
Authors: Xuan Zuodong , Chen Chen , Tang Wenbin , Ye Shaopei , Zheng Jianzhong , Zhao Yue , Shi Zhiyuan , Zhang Lei , Sun Huimin , Shao Chen
Published: June 2021

Tyrosine kinase inhibitors (TKI)-resistant renal cancer is highly susceptible to metastasis, and enhanced vascular permeability promotes the process of metastasis. To evaluate the effect of cancer-derived exosomes on vascular endothelial cells and clarify the mechanism of metastasis in TKI-resistant renal cancer, we studied the crosstalk between clear cell renal cell carcinoma (ccRCC) cells and human umbilical vein endothelial cells (HUVECs). Exosomes from ccRCC cells enhanced the expression of vascular permeability-related proteins. Compared with sensitive strains, exosomes from resistant strains significantly enhanced vascular endothelial permeability, induced tumor angiogenesis and enhanced tumor lung metastasis in nude mice. The expression of miR-549a is lower in TKI-resistant cells and exosomes, which enhanced the expression of HIF1α in endothelial cells. In addition, TKI-resistant RCC cells reduced nuclear output of pre-miR-549a via the VEGFR2-ERK-XPO5 pathway, and reduced enrichment of mature miR-549a in cytoplasm, which in turn promoted HIF1α expression in RCC, leading to increased VEGF secretion and further activated VEGFR2 to form a feedback effect. miR-549a played an important role in the metastasis of renal cancer and might serve as a blood biomarker for ccRCC metastasis and even had the potential of becoming a new drug to inhibit TKI-resistance.
Oleic Acid Protects Caenorhabditis Mothers From Mating-Induced Death and the Cost of Reproduction
Authors: Choi Leo S. , Shi Cheng , Ashraf Jasmine , Sohrabi Salman , Murphy Coleen T.
Published: June 2021

Reproduction comes at a cost, including accelerated death. Previous studies of the interconnections between reproduction, lifespan, and fat metabolism in C. elegans were predominantly performed in low-reproduction conditions. To understand how increased reproduction affects lifespan and fat metabolism, we examined mated worms; we find that a Δ9 desaturase, FAT-7, is significantly up-regulated. Dietary supplementation of oleic acid (OA), the immediate downstream product of FAT-7 activity, restores fat storage and completely rescues mating-induced death, while other fatty acids cannot. OA-mediated lifespan restoration is also observed in C. elegans mutants suffering increased death from short-term mating, and in mated C. remanei females, indicating a conserved role of oleic acid in post-mating lifespan regulation. Our results suggest that increased reproduction can be uncoupled from the costs of reproduction from somatic longevity regulation if provided with the limiting lipid, oleic acid.
The Calcium Binding Protein S100A11 and Its Roles in Diseases
Authors: Zhang Linqiang , Zhu Tingting , Miao Huilai , Liang Bin
Published: June 2021

The calcium binding protein S100 family in humans contains 21 known members, with each possessing a molecular weight between 10 and 14 kDa. These proteins are characterized by a unique helix-loop-helix EF hand motif, and often form dimers and multimers. The S100 family mainly exists in vertebrates and exerts its biological functions both inside cells as a calcium sensor/binding protein, as well as outside cells. S100A11, a member of the S100 family, may mediate signal transduction in response to internal or external stimuli and it plays various roles in different diseases such as cancers, metabolic disease, neurological diseases, and vascular calcification. In addition, it can function as chemotactic agent in inflammatory disease. In this review, we first detail the discovery of S100 proteins and their structural features, and then specifically focus on the tissue and organ expression of S100A11. We also summarize its biological activities and roles in different disease and signaling pathways, providing an overview of S100A11 research thus far.
Crystallization Control of N,N′-Dioctyl Perylene Diimide by Amphiphilic Block Copolymers Containing poly(3-Hexylthiophene) and Polyethylene Glycol
Authors: Yang Xiaohui , Lu Wanlong , Cao Jingning , Zhai Chenyang , Li Weili , Zha Fangwen , Lu Guanghao , Tian Hongkun , Yu Demei , Bu Laju
Published: June 2021

The preparation of micron- to nanometer-sized functional materials with well-defined shapes and packing is a key process to their applications. There are many ways to control the crystal growth of organic semiconductors. Adding polymer additives has been proven a robust strategy to optimize semiconductor crystal structure and the corresponding optoelectronic properties. We have found that poly(3-hexylthiophene) (P3HT) can effectively regulate the crystallization behavior of N,N′-dioctyl perylene diimide (C8PDI). In this study, we combined P3HT and polyethylene glycol (PEG) to amphiphilic block copolymers and studied the crystallization modification effect of these block copolymers. It is found that the crystallization modification effect of the block copolymers is retained and gradually enhanced with P3HT content. The length of C8PDI crystals were well controlled from 2 to 0.4 μm, and the width from 210 to 35 nm. On the other hand, due to the water solubility of PEG block, crystalline PEG-b-P3HT/C8PDI micelles in water were successfully prepared, and this water phase colloid could be stable for more than 2 weeks, which provides a new way to prepare pollution-free aqueous organic semiconductor inks for printing electronic devices.
A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin
Authors: Soylu H. Melis , Chevallier Pascale , Copes Francesco , Ponti Federica , Candiani Gabriele , Yurt Fatma , Mantovani Diego
Published: June 2021

Introduction

The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site. Antibacterial coatings have been proposed as a viable solution to limit such infections. However, despite being effective at short-term, conventional coatings lack the ability to prevent infections at medium and long-term. Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration. Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations. In this study, an agarose hydrogel-based coating has been developed for the controlled release of gentamicin (GS).

Methods

Sand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution. After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers. The different GS-loaded hydrogel formulations were deposited on Ti6Al4V-DOPA surfaces, and allowed to react under UV irradiation. Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses. GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test. The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.

Results

A homogeneous coating was obtained with all the hydrogel formulations. Moreover, the coatings presented a hydrophilic behavior and micro-scale surface roughness. The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels. Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels. The addition of CaCl2 to the gel formulation was able to significantly decrease cytotoxicity of the TA-modified hydrogels.

Conclusions

Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.
Current Devices and Complications Related to Transcatheter Mitral Valve Replacement: The Bumpy Road to the Top
Authors: Kargoli Faraj , Pagnesi Matteo , Rahgozar Kusha , Goldberg Ythan , Ho Edwin , Chau Mei , Colombo Antonio , Latib Azeem
Published: June 2021

Mitral regurgitation is the most common valvular lesion in the developed world, with increasing prevalence, morbidity, and mortality. The experience with surgical mitral valve repair or replacement is very well-validated. However, more than 45% of these patients get denied surgery due to an elevated risk profile and advanced disease of the left ventricle at the time of presentation, promoting the need for less invasive transcatheter options such as transcatheter repair and transcatheter mitral valve replacement (TMVR). Early available TMVR studies have shown promising results, and several dedicated devices are under clinical evaluation. However, TMVR is still in the early developmental stages and is associated with a non-negligible risk of periprocedural and post-procedural complications. In this review, we discuss the current challenges facing TMVR and the potential TMVR-related complications, offering an overview on the measures implemented to mitigate these complications, and future implications.
Single-Cell Transcriptomics Reveals the Cellular Heterogeneity of Cardiovascular Diseases
Authors: Fu Mengxia , Song Jiangping
Published: June 2021

“A world in a wild flower, and a bodhi in a leaf,” small cells contain huge secrets. The vasculature is composed of many multifunctional cell subpopulations, each of which is involved in the occurrence and development of cardiovascular diseases. Single-cell transcriptomics captures the full picture of genes expressed within individual cells, identifies rare or de novo cell subpopulations, analyzes single-cell trajectory and stem cell or progenitor cell lineage conversion, and compares healthy tissue and disease-related tissue at single-cell resolution. Single-cell transcriptomics has had a profound effect on the field of cardiovascular research over the past decade, as evidenced by the construction of cardiovascular cell landscape, as well as the clarification of cardiovascular diseases and the mechanism of stem cell or progenitor cell differentiation. The classification and proportion of cell subpopulations in vasculature vary with species, location, genotype, and disease, exhibiting unique gene expression characteristics in organ development, disease progression, and regression. Specific gene markers are expected to be the diagnostic criteria, therapeutic targets, or prognostic indicators of diseases. Therefore, treatment of vascular disease still has lots of potentials to develop. Herein, we summarize the cell clusters and gene expression patterns in normal vasculature and atherosclerosis, aortic aneurysm, and pulmonary hypertension to reveal vascular heterogeneity and new regulatory factors of cardiovascular disease in the use of single-cell transcriptomics and discuss its current limitations and promising clinical potential.