bluuebunny/crossref_metadata_2025 huggingface dataset:
Factors Involved in the Functional Motor Recovery of Rats with Cortical Ablation after GH and Rehabilitation Treatment: Cortical Cell Proliferation and Nestin and Actin Expression in the Striatum and Thalamus
Authors: Heredia Margarita , Rodríguez Natalia , Sánchez Robledo Virginia , Criado José María , de la Fuente Antonio , Devesa Jesús , Devesa Pablo , Sánchez Riolobos Adelaida
Published: November 2019

Previously we demonstrated, in rats, that treatment with growth hormone (GH) and rehabilitation, carried out immediately after a motor cortical ablation, significantly improved the motor affectation produced by the lesion and induced the re-expression of nestin in the contralateral motor cortex. Here we analyze cortical proliferation after ablation of the frontal motor cortex and investigate the re-expression of nestin in the contralateral motor cortex and the role of the striatum and thalamus in motor recovery. The rats were subjected to ablation of the frontal motor cortex in the dominant hemisphere or sham-operated and immediately treated with GH or the vehicle (V), for five days. At 1 dpi (days post-injury), all rats received daily injections (for four days) of bromodeoxyuridine and five rats were sacrificed at 5 dpi. The other 15 rats (n = 5/group) underwent rehabilitation and were sacrificed at 25 dpi. GH induced the greatest number of proliferating cells in the perilesional cortex. GH and rehabilitation produced the functional recovery of the motor lesion and increased the expression of nestin in the striatum. In the thalamic ventral nucleus ipsilateral to the lesion, cells positive for nestin and actin were detected, but this was independent on GH. Our data suggest that GH-induced striatal nestin is involved in motor recovery.
Quercetin Exposure Suppresses the Inflammatory Pathway in Intestinal Organoids from Winnie Mice
Authors: Dicarlo Manuela , Teti Gabriella , Verna Giulio , Liso Marina , Cavalcanti Elisabetta , Sila Annamaria , Raveenthiraraj Sathuwarman , Mastronardi Mauro , Santino Angelo , Serino Grazia , Lippolis Antonio , Sobolewski Anastasia , Falconi Mirella , Chieppa Marcello
Published: November 2019

Inflammatory bowel diseases (IBDs) are chronic and relapsing immune disorders that result, or possibly originate, from epithelial barrier defects. Intestinal organoids are a new reliable tool to investigate epithelial response in models of chronic inflammation. We produced organoids from the ulcerative colitis murine model Winnie to explore if the chronic inflammatory features observed in the parental intestine were preserved by the organoids. Furthermore, we investigated if quercetin administration to in vitro cultured organoids could suppress LPS-induced inflammation in wild-type organoids (WT-organoids) and spontaneous inflammation in ulcerative colitis organoids (UC-organoids). Our data demonstrate that small intestinal organoids obtained from Winnie mice retain the chronic intestinal inflammatory features characteristic of the parental tissue. Quercetin administration was able to suppress inflammation both in UC-organoids and in LPS-treated WT-organoids. Altogether, our data demonstrate that UC-organoids are a reliable experimental system for investigating chronic intestinal inflammation and pharmacological responses.
Contribution of Single-Cell Transcriptomics to the Characterization of Human Spermatogonial Stem Cells: Toward an Application in Male Fertility Regenerative Medicine?
Authors: Gille Anne-Sophie , Lapoujade Clémentine , Wolf Jean-Philippe , Fouchet Pierre , Barraud-Lange Virginie
Published: November 2019

Ongoing progress in genomic technologies offers exciting tools that can help to resolve transcriptome and genome-wide DNA modifications at single-cell resolution. These methods can be used to characterize individual cells within complex tissue organizations and to highlight various molecular interactions. Here, we will discuss recent advances in the definition of spermatogonial stem cells (SSC) and their progenitors in humans using the single-cell transcriptome sequencing (scRNAseq) approach. Exploration of gene expression patterns allows one to investigate stem cell heterogeneity. It leads to tracing the spermatogenic developmental process and its underlying biology, which is highly influenced by the microenvironment. scRNAseq already represents a new diagnostic tool for the personalized investigation of male infertility. One may hope that a better understanding of SSC biology could facilitate the use of these cells in the context of fertility preservation of prepubertal children, as a key component of regenerative medicine.
Pyruvate Dehydrogenase and Tricarboxylic Acid Cycle Enzymes Are Sensitive Targets of Traumatic Brain Injury Induced Metabolic Derangement
Authors: Lazzarino Giacomo , Amorini Angela Maria , Signoretti Stefano , Musumeci Giuseppe , Lazzarino Giuseppe , Caruso Giuseppe , Pastore Francesco Saverio , Di Pietro Valentina , Tavazzi Barbara , Belli Antonio
Published: November 2019

Using a closed-head impact acceleration model of mild or severe traumatic brain injury (mTBI or sTBI, respectively) in rats, we evaluated the effects of graded head impacts on the gene and protein expressions of pyruvate dehydrogenase (PDH), as well as major enzymes of mitochondrial tricarboxylic acid cycle (TCA). TBI was induced in anaesthetized rats by dropping 450 g from 1 (mTBI) or 2 m height (sTBI). After 6 h, 12 h, 24 h, 48 h, and 120 h gene expressions of enzymes and subunits of PDH. PDH kinases and phosphatases (PDK1-4 and PDP1-2, respectively), citrate synthase (CS), isocitrate dehydrogenase (IDH), oxoglutarate dehydrogenase (OGDH), succinate dehydrogenase (SDH), succinyl-CoA synthase (SUCLG), and malate dehydrogenase (MDH) were determined in whole brain extracts (n = 6 rats at each time for both TBI levels). In the same samples, the high performance liquid chromatographic (HPLC) determination of acetyl-coenzyme A (acetyl-CoA) and free coenzyme A (CoA-SH) was performed. Sham-operated animals (n = 6) were used as controls. After mTBI, the results indicated a general transient decrease, followed by significant increases, in PDH and TCA gene expressions. Conversely, permanent PDH and TCA downregulation occurred following sTBI. The inhibitory conditions of PDH (caused by PDP1-2 downregulations and PDK1-4 overexpression) and SDH appeared to operate only after sTBI. This produced almost no change in acetyl-CoA and free CoA-SH following mTBI and a remarkable depletion of both compounds after sTBI. These results again demonstrated temporary or steady mitochondrial malfunctioning, causing minimal or profound modifications to energy-related metabolites, following mTBI or sTBI, respectively. Additionally, PDH and SDH appeared to be highly sensitive to traumatic insults and are deeply involved in mitochondrial-related energy metabolism imbalance.
Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress
Authors: Li Yong-Bo , Cui De-Zhou , Sui Xin-Xia , Huang Chen , Huang Cheng-Yan , Fan Qing-Qi , Chu Xiu-Sheng
Published: November 2019

Although studies have shown the concomitant occurrence of autophagic and programmed cell death (PCD) in plants, the relationship between autophagy and PCD and the factors determining this relationship remain unclear. In this study, seedlings of the wheat cultivar Jimai 22 were used to examine the occurrence of autophagy and PCD during polyethylene glycol (PEG)-8000-induced drought stress. Autophagy and PCD occurred sequentially, with autophagy at a relatively early stage and PCD at a much later stage. These findings suggest that the duration of drought stress determines the occurrence of PCD following autophagy. Furthermore, the addition of 3-methyladenine (3-MA, an autophagy inhibitor) and the knockdown of autophagy-related gene 6 (ATG6) accelerated PEG-8000-induced PCD, respectively, suggesting that inhibition of autophagy also results in PCD under drought stress. Overall, these findings confirm that wheat seedlings undergo autophagic survival under mild drought stress, with subsequent PCD only under severe drought.
Pentraxin-3 Modulates Osteogenic/Odontogenic Differentiation and Migration of Human Dental Pulp Stem Cells
Authors: Kim Yeon , Park Joo-Yeon , Park Hyun-Joo , Kim Mi-Kyoung , Kim Yong-Il , Kim Hyung Joon , Bae Soo-Kyung , Bae Moon-Kyoung
Published: November 2019

Pentraxin-3 (PTX3) is recognized as a modulator of inflammation and a mediator of tissue repair. In this study, we characterized the role of PTX3 on some biological functions of human dental pulp stem cells (HDPSCs). The expression level of PTX3 significantly increased during osteogenic/odontogenic differentiation of HDPSCs, whereas the knockdown of PTX3 decreased this differentiation. Silencing of PTX3 in HDPSCs inhibited their migration and C-X-C chemokine receptor type 4 (CXCR4) expression. Our present study indicates that PTX3 is involved in osteogenic/odontogenic differentiation and migration of HDPSCs, and may contribute to the therapeutic potential of HDPSCs for regeneration and repair.
The Dichotomous Nature of AZ5104 (an EGFR Inhibitor) Towards RORγ and RORγT
Authors: Karaś Kaja , Sałkowska Anna , Karwaciak Iwona , Walczak-Drzewiecka Aurelia , Dastych Jarosław , Bachorz Rafał A. , Ratajewski Marcin
Published: November 2019

The RORC (RAR related orphan receptor C) gene produces two isoforms by alternative promoter usage: RORγ (nuclear receptor ROR-gamma isoform 1) and RORγT (nuclear receptor ROR-gamma isoform 1). Both proteins have distinct tissue distributions and are involved in several physiological processes, including glucose/lipid metabolism and the development of Th17 lymphocytes. Previously, we developed a stably transfected reporter cell line and used it to screen a library of kinase inhibitors. We found that AZ5104 acts as an RORγ agonist at low micromolar concentrations. Molecular docking analysis showed that this compound occupies the ligand binding domain of the receptor with a significant docking score. However, analysis of the biological activity of this compound in Th17 cells revealed that it downregulates RORγT expression and Th17-related cytokine production via inhibition of SRC-ERK-STAT3 (SRC proto-oncogene - extracellular regulated MAP kinase - signal transducer and activator of transcription 3). We thus identified a compound acting as an agonist of RORγ that, due to the inhibition of downstream elements of EGFR (epidermal growth factor receptor) signaling, exerts different biological activity towards a Th17-specific isoform. Additionally, our results may be relevant in the future for the design of treatments targeting signaling pathways that inhibit Th17-related inflammation in certain autoimmune disorders.
p63 Is a Promising Marker in the Diagnosis of Unusual Skin Cancer
Authors: Smirnov Artem , Anemona Lucia , Novelli Flavia , Piro Cristina M. , Annicchiarico-Petruzzelli Margherita , Melino Gerry , Candi Eleonora
Published: November 2019

Skin cancer is the most common type of cancer worldwide. Ozone depletion and climate changes might cause a further increase in the incidence rate in the future. Although the early detection of skin cancer enables it to be treated successfully, some tumours can evolve and become more aggressive, especially in the case of melanoma. Therefore, good diagnostic and prognostic markers are needed to ensure correct detection and treatment. Transcription factor p63, a member of the p53 family of proteins, plays an essential role in the development of stratified epithelia such as skin. In this paper, we conduct a comprehensive review of p63 expression in different types of skin cancer and discuss its possible use in the diagnosis and prognosis of cutaneous tumours.
Inositols’ Importance in the Improvement of the Endocrine–Metabolic Profile in PCOS
Authors: Wojciechowska Anna , Osowski Adam , Jóźwik Marcin , Górecki Ryszard , Rynkiewicz Andrzej , Wojtkiewicz Joanna
Published: November 2019

Polycystic ovary syndrome (PCOS) is one of the most common causes of infertility and metabolic problems among women of reproductive age. The mechanism of PCOS is associated with concurrent alterations at the hormonal level. The diagnosis assumes the occurrence of three interrelated symptoms of varying severity, namely ovulation disorders, androgen excess, or polycystic ovarian morphology (PCOM), which all require a proper therapeutic approach. The main symptom seems to be an increased androgen concentration, which in turn may contribute to different metabolic disorders. A number of papers have demonstrated the significant role of inositol therapy in PCOS. However, there is a lack of detailed discussion about the importance of myo-inositol (MI) and d-chiro-inositol (DCI) in reference to particular symptoms. Thus, the aim of this review is to present the effectiveness of MI and DCI treatment for PCOS symptoms. Moreover, the review is focused on analyzing the use of inositols, taking into account their physiological properties, together with the mechanism of individual PCOS symptom formation.
Encapsulation of Lovastatin in Zein Nanoparticles Exhibits Enhanced Apoptotic Activity in HepG2 Cells
Authors: Alhakamy Nabil A. , Ahmed Osama A.A. , Aldawsari Hibah M. , Alfaifi Mohammad Y. , Eid Basma G. , Abdel-Naim Ashraf B. , Fahmy Usama A.
Published: November 2019

Research on statins highlights their potent cytotoxicity against cancer cells and their potential for cancer prevention. The aim of the current study was to examine whether loading lovastatin (LVS) in zein (ZN) nanoparticles (NPs) would potentiate the anti-proliferative effects of LVS and enhance its proliferation-inhibiting activity in HepG2 cells. LVS-ZN NPs were prepared and showed excellent characteristics, with respect to their particle size, zeta potential, diffusion, and entrapment efficiency. In addition, they showed the most potent anti-proliferative activity against HepG2 cells. ZN alone showed an observable anti-proliferative that was significantly higher than that of raw LVS. Furthermore, LVS uptake by HepG2 cells was greatly enhanced by the formulation in ZN. A cell cycle analysis indicated that LVS induced a significant cell accumulation in the G2/M and pre-G phases. In this regard, the LVS–ZN NPs exhibited the highest potency. The accumulation in the pre-G phase indicated an enhanced pro-apoptotic activity of the prepared formula. The cells incubated with the LVS-ZN NPs showed the highest percentage of cells with annexin-V positive staining. In addition, the same incubations showed the highest content of caspase-3 enzyme in comparison to raw LVS or ZN. Thus, the loading of LVS in ZN nanoparticles enhances its anti-proliferative activity against HepG2 cells, which is attributed, at least partly, to the enhanced cellular uptake and the induction of apoptosis.
Tellimagrandin II, A Type of Plant Polyphenol Extracted from Trapa bispinosa Inhibits Antibiotic Resistance of Drug-Resistant Staphylococcus aureus
Authors: Chang Yu-Wei , Huang Wan-Chun , Lin Chun-Yu , Wang Wen-Hung , Hung Ling-Chien , Chen Yen-Hsu
Published: November 2019

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become a critical global concern. Identifying new candidates of anti-S. aureus agents is urgently required because the therapeutic strategies for infected patients are limited currently. Therefore, the present study investigated whether Tellimagrandin II (TGII), a pure compound extracted from the shells of Trapa bispinosa, exhibits antibacterial effects against MRSA. We first showed that TGII exerted potent inhibitory activity against MRSA with a minimum inhibitory concentration of 128 μg/mL. The obtained fractional inhibitory concentration suggested that TGII could alone exert antistaphylococcal activity, and TGII combined with low doses of antibiotics displayed synergistic effects against MRSA. Moreover, we found that TGII exerted bactericidal activity by reducing the expression of mecA followed by the negative regulation of the penicillin-binding protein 2a (PBP2a) of MRSA. Transmission electron microscopy (TEM) images further confirmed that TGII destroyed the integrity of the cell wall of MRSA and caused the loss of cytoplasm content. In conclusion, we evidenced the antibacterial effects of TGII against MRSA, which enables the effective dose of current antibiotics to be reduced and the predicament of drug-resistant S. aureus isolates to be overcome.
PI3K/mTOR Pathway Inhibition: Opportunities in Oncology and Rare Genetic Diseases
Authors: Hillmann Petra , Fabbro Doriano
Published: November 2019

The phosphatidylinositol 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) signaling pathway has been implicated as a cancer target. Big pharma players and small companies have been developing small molecule inhibitors of PI3K and/or mTOR since the 1990s. Although four inhibitors have been approved, many open questions regarding tolerability, patient selection, sensitivity markers, development of resistances, and toxicological challenges still need to be addressed. Besides clear oncological indications, PI3K and mTOR inhibitors have been suggested for treating a plethora of different diseases. In particular, genetically induced PI3K/mTOR pathway activation causes rare disorders, known as overgrowth syndromes, like PTEN (phosphatase and tensin homolog) hamartomas, tuberous sclerosis complex (TSC), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA)-related overgrowth spectrum (PROS), and activated PI3-Kinase delta syndrome (PI3KCD, APDS). Some of those disorders likeTSC or hemimegalencephaly, which are one of the PROS disorders, also belong to a group of diseases called mTORopathies. This group of syndromes presents with additional neurological manifestations associated with epilepsy and other neuropsychiatric symptoms induced by neuronal mTOR pathway hyperactivation. While PI3K and mTOR inhibitors have been and still are intensively tested in oncology indications, their use in genetically defined syndromes and mTORopathies appear to be promising avenues for a pharmacological intervention.
The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome
Authors: AbdelMageed Manar , Ali Haytham , Olsson Lina , Lindmark Gudrun , Hammarström Marie-Louise , Hammarström Sten , Sitohy Basel
Published: November 2019

Chemokines are important in the development and progression of tumors. We investigated the expression of CXCL14 and CXCL16 in colon cancer. Expression of mRNA was assessed in primary tumors and lymph nodes and CXCL16 mRNA levels were correlated to patient’s survival. Protein expression was investigated by two-color immunofluorescence and immunomorphometry. CXCL14 and CXCL16 mRNA levels and protein expression were significantly higher in colon cancer primary tumors compared to apparently normal colon tissue. Positive cells were tumor cells, as revealed by anti-CEA and anti-EpCAM staining. CXCL16, but not CXCL14, mRNA levels were significantly higher in hematoxylin and eosin positive (H&E(+)) compared to H&E(−) colon cancer lymph nodes or control nodes (P < 0.0001). CXCL16 mRNA was expressed in 5/5 colon cancer cell lines while CXCL14 was expressed significantly in only one. Kaplan-Meier analysis revealed that colon cancer patients with lymph nodes expressing high or very high levels (7.2 and 11.4 copies/18S rRNA unit, respectively) of CXCL16 mRNA had a decreased mean survival time of 30 and 46 months at the 12-year follow-up (P = 0.04, P = 0.005, respectively). In conclusion, high expression of CXCL16 mRNA in regional lymph nodes of colon cancer patients is a sign of a poor prognosis.
B Vitamins and Fatty Acids: What Do They Share with Small Vessel Disease-Related Dementia?
Authors: Moretti Rita , Peinkhofer Costanza
Published: November 2019

Many studies have been written on vitamin supplementation, fatty acid, and dementia, but results are still under debate, and no definite conclusion has yet been drawn. Nevertheless, a significant amount of lab evidence confirms that vitamins of the B group are tightly related to gene control for endothelium protection, act as antioxidants, play a co-enzymatic role in the most critical biochemical reactions inside the brain, and cooperate with many other elements, such as choline, for the synthesis of polyunsaturated phosphatidylcholine, through S-adenosyl-methionine (SAM) methyl donation. B-vitamins have anti-inflammatory properties and act in protective roles against neurodegenerative mechanisms, for example, through modulation of the glutamate currents and a reduction of the calcium currents. In addition, they also have extraordinary antioxidant properties. However, laboratory data are far from clinical practice. Many studies have tried to apply these results in everyday clinical activity, but results have been discouraging and far from a possible resolution of the associated mysteries, like those represented by Alzheimer’s disease (AD) or small vessel disease dementia. Above all, two significant problems emerge from the research: No consensus exists on general diagnostic criteria—MCI or AD? Which diagnostic criteria should be applied for small vessel disease-related dementia? In addition, no general schema exists for determining a possible correct time of implementation to have effective results. Here we present an up-to-date review of the literature on such topics, shedding some light on the possible interaction of vitamins and phosphatidylcholine, and their role in brain metabolism and catabolism. Further studies should take into account all of these questions, with well-designed and world-homogeneous trials.
Interactive Effects of Salicylic Acid and Nitric Oxide in Enhancing Rice Tolerance to Cadmium Stress
Authors: Mostofa Mohammad Golam , Rahman Md. Mezanur , Ansary Md. Mesbah Uddin , Fujita Masayuki , Tran Lam-Son Phan
Published: November 2019

Cadmium (Cd) is one of the prominent environmental hazards, affecting plant productivity and posing human health risks worldwide. Although salicylic acid (SA) and nitric oxide (NO) are known to have stress mitigating roles, little was explored on how they work together against Cd-toxicity in rice. This study evaluated the individual and combined effects of SA and sodium nitroprusside (SNP), a precursor of NO, on Cd-stress tolerance in rice. Results revealed that Cd at toxic concentrations caused rice biomass reduction, which was linked to enhanced accumulation of Cd in roots and leaves, reduced photosynthetic pigment contents, and decreased leaf water status. Cd also potentiated its phytotoxicity by triggering reactive oxygen species (ROS) generation and depleting several non-enzymatic and enzymatic components in rice leaves. In contrast, SA and/or SNP supplementation with Cd resulted in growth recovery, as evidenced by greater biomass content, improved leaf water content, and protection of photosynthetic pigments. These signaling molecules were particularly effective in restricting Cd uptake and accumulation, with the highest effect being observed in “SA + SNP + Cd” plants. SA and/or SNP alleviated Cd-induced oxidative damage by reducing ROS accumulation and malondialdehyde production through the maintenance of ascorbate and glutathione levels, and redox status, as well as the better activities of antioxidant enzymes superoxide dismutase, catalase, glutathione S-transferase, and monodehydroascorbate reductase. Combined effects of SA and SNP were observed to be more prominent in Cd-stress mitigation than the individual effects of SA followed by that of SNP, suggesting that SA and NO in combination more efficiently boosted physiological and biochemical responses to alleviate Cd-toxicity than either SA or NO alone. This finding signifies a cooperative action of SA and NO in mitigating Cd-induced adverse effects in rice, and perhaps in other crop plants.
An Update on Antibodies to Necleosome Components as Biomarkers of Sistemic Lupus Erythematosus and of Lupus Flares
Authors: Ghiggeri Gian Marco , D’Alessandro Matteo , Bartolomeo Domenico , Degl’Innocenti Maria Ludovica , Magnasco Alberto , Lugani Francesca , Prunotto Marco , Bruschi Maurizio
Published: November 2019

Systemic lupus erythematosus (SLE) is an autoimmune disease with variable clinical expression. It is a potentially devastating condition affecting mostly women and leading to clinically unpredictable outcomes. Remission and flares may, in fact, alternate over time and a mild involvement limited to few articular sites may be followed by severe and widespread organ damage. SLE is the prototype of any autoimmune condition and has, for this reason, attracted the interest of basic immunologists. Therapies have evolved over time and clinical prognosis has, in parallel, been improved. What clinicians still lack is the possibility to use biomarkers of the disease as predictors of outcome and, in this area, several studies are trying to find solutions. Circulating autoantibodies are clearly a milestone of clinical research and the concrete possibility is to integrate, in the future, classical markers of activation (like C3) with target organ autoantibodies. Anti-dsDNA antibodies represent a basic point in any predictive attempt in SLE and should be considered the benchmark for any innovative proposal in the wide field of target organ pathologies related to SLE. DNA is part of the nucleosome that is the basic unit of chromatin. It consists of DNA wrapped around a histone octamer made of 2 copies each of Histone 2A, 2B, 3, and 4. The nucleosome has a plastic organization that varies over time and has the potential to stimulate the formation of antibodies directed to the whole structure (anti-nucleosome) or its parts (anti-dsDNA and anti-Histones). Here, we present an updated review of the literature on antibodies directed to the nucleosome and the nucleosome constituents, i.e., DNA and Histones. Wetriedto merge the data first published more than twenty years ago with more recent results to create a balanced bridge between old dogma and more recent research that could serve as a stimulus to reconsider mechanisms for SLE. The formation of large networks would provide the chance of studying large cohorts of patients and confirm what already presented in small sample size during the last years.
Management of High-Pressure Injection Hand Injuries: A Multicentric, Retrospective, Observational Study
Authors: Vitale Ermanno , Ledda Caterina , Adani Roberto , Lando Mario , Bracci Massimo , Cannizzaro Emanuele , Tarallo Luigi , Rapisarda Venerando
Published: November 2019

Hand injuries after high-pressure injection are a medical emergency. These events occur frequently in workers during industrial cleaning, painting, and lubrication, and may have devastating consequences, leading to eventual amputation and poor functional outcomes. The authors have investigated the evolution, management, and outcome. Medical records of occupational medicine units and hand surgery units were collected in order to spot the high-pressure gear accident cases. Records were analyzed by dividing the subjects into two groups: those treated within 6 h and after 6 h of the trauma. A follow-up was carried out at least 1 year after treatment; the post-treatment outcomes were assessed. Of the 71 (100%) subjects, 26 (37%) were treated ≤6 h and 45 (63%) >6 h. A total of 28% (n = 20) underwent amputation. In 61% of cases, accidents had occurred in the iron and steel sector. High viscosity materials with a delayed treatment beyond 6 h seemed to determine compartmental syndrome and following amputation. A significantly better outcome was reported among subjects treated ≤6 h compared to those treated >6 h, 20% (n = 7) versus 26% (n = 9), respectively. Early management of this type of injury is crucial. The results of this study may contribute to providing guidelines to occupational physicians in order to best manage this type of emergency.
Biomechanical Properties and Biocompatibility of a Non-Absorbable Elastic Thread
Authors: Choi Yeji , Kang Moonseok , Choi Moon Seop , Kim Song Jennifer , Lih Eugene , Lee Deahyung , Jung Hong-Hee
Published: November 2019

To date, extensive studies have been conducted to assess diverse types of sutures. But there is a paucity of data regarding biomechanical properties of commonly used suture materials. In the current experiment, we compared biomechanical properties and biocompatibility, such as tensile strength and elongation, the degree of bovine serum albumin (BSA) release, in vitro cytotoxicity and ex vivo frictional properties, between a non-absorbable elastic thread (NAT; HansBiomed Co. Ltd., Seoul, Korea) (NAT-R: NAT with a rough surface, NAT-S: NAT with a smooth surface) and the Elasticum® (Korpo SRL, Genova, Italy). The degree of tensile strength and elongation of Si threads was significantly higher in both the NAT-R and -S as compared with the Elasticum® (p < 0.05). Moreover, the degree of tensile strength and elongation of PET threads was significantly lower in both NAT-R and -S as compared with the Elasticum® (p < 0.05). Furthermore, the degree of tensile strength and elongation of braided Si/PET threads was significantly lower in NAT-S as compared with NAT-R and Elasticum® (p < 0.05). The degree of BSA release was significantly higher in the NAT-R as compared with Elasticum® and NAT-S throughout a 2-h period in the descending order (p < 0.05). The degree of cell viability was significantly higher in both NAT-R and -S as compared with Elasticum® (p < 0.05). The degree of coefficient of friction as well as the frictional force and strength was significantly higher in NAT-R as compared with NAT-S and Elasticum® (p < 0.05). NAT had a higher degree of biomechanical properties and biocompatibility as compared with Elasticum®. But further experimental and clinical studies are warranted to compare the efficacy, safety, and potential role as a carrier for drug delivery between NAT and Elasticum®.
Usage of Vertical Fisheye-Images to Quantify Urban Light Pollution on Small Scales and the Impact of LED Conversion
Authors: Wallner Stefan
Published: November 2019

The aim of this work was to develop an easy and quick technique for characterizing various lighting situations, that is, single lamps or illuminated signs and to quantify impacts on small scales like streets, buildings and near areas. The method uses a DSLR-camera equipped with fisheye-lens and the software Sky Quality Camera, both commonly used as part of night sky imagery in the light pollution community, to obtain information about luminance and correlated colour temperature. As a difference to its usual build-up, observed light emitting sources were captured by pointing the camera towards analysed objects, that is, images were taken via vertical plane imaging with very short exposure times under one second. Results have proven that this technique provides a practical way to quantify the lighting efficacy in a certain place or area, as a quantitative analysis of the direct emission towards the observer and the illumination on surroundings, that is, street surfaces, sidewalks and buildings, was performed. When conducting lamp conversions, the method can be used to characterize the gradient of change and could be a useful tool for municipalities to find the optimal lighting solution. The paper shows examples of different lighting situations like single lamps of different types, also containing various luminaires, illuminated billboards or buildings and impacts of the lighting transition to LEDs in the city of Eisenstadt, Austria. The horizontal fisheye method is interdisciplinary applicable, for example, being suitable for lighting management, to sustainability and energy saving purposes.
Wave Energy Disbalance as Generator of Extreme Wave Occurrence in Semi-Enclosed Coastal Waters (Example of Rijeka Bay—Croatia)
Authors: Lončar Goran , Leder Nenad , Duplančić Leder Tea , Carević Dalibor
Published: November 2019

The conditions for the occurrence of high waves in front of the Rijeka port in the Rijeka Bay were analyzed. The analysis was carried out on the basis of measured data on the wave rider station located in front of the main breakwater of the port of Rijeka and the results of numerical wave generation modelings for the wider sea area on the spatial scale of the Adriatic basin. The results of the conducted analysis show that the sudden transition in wind direction from the third to the second quadrant (and vice versa), with the simultaneous rapid increase in wind speed, creates the conditions for generating the largest waves in front of the port of Rijeka. The main reason for achieving the highest wave height in these conditions is the unbalanced wind power input with non-developed surface dissipation (white-capping) and quadruplet wave interaction. Situations with a slower increase in wind speed and approximately constant wind direction resulted in the occurrence of smaller wave heights. The direct application of anemometric data for the forcing wind field in the Adriatic basin within the wave generation model results in a more accurate simulation of wave height and wave period development than application of the wind field from the prediction atmospheric model Aladin-Hr. This is due to the fact that the site is located in a semi-enclosed sea area of restricted fetch, and the spatial/temporal resolution of atmospheric data (2 km and 3 h) is not sufficient to resolve the rapid transition in the wind field. In the case of direct application of anemometric data, the white-capping parameterization should be of a non-stationary character.
The Protection of Fashion Shows: An Uncharted Stage
Authors: Varese Elena , Mazza Valentina
Published: November 2019

The history of fashion shows goes back more than a century, and over the years, catwalks have gone from being private sales channels for a few wealthy customers to pure entertainment shows promoted globally. In this article, we analyze both national and international laws dealing with the protection of fashion shows in order to establish how fashion shows could be protected under intellectual property laws in Italy, with specific regard to copyright. The possibility for fashion shows to access copyright protection opens up a list of interesting questions: Who is the author of the work? Are models to be considered as performers? This scenario gets even more complicated if we consider how fashion shows have been changing in the last few years, turning to new technologies such as holograms, augmented reality, and drones. Further, copyright protection could be accompanied by further tools, such as registered or unregistered designs for the single elements of a scene and choreography or unfair competition if the general look and feel of a former fashion show has been slavishly imitated. A final section of this article is dedicated to the use of cultural heritage and historical museums, which are increasingly chosen by fashion houses for the settings of their shows.
Mechanical Properties of Nano SiO2 and Carbon Fiber Reinforced Concrete after Exposure to High Temperatures
Authors: Wu Linsong , Lu Zhenhui , Zhuang Chenglong , Chen Yu , Hu Ruihua
Published: November 2019

This study presents the key mechanical and residual properties after high-temperature of different Nano SiO2 carbon fiber-reinforced concrete (NSCFRC) mixtures. A total of seven NSCFRC mixtures incorporating 0%–0.35% of carbon fiber by volume of concrete and 0%–2% Nano SiO2 by weight of the binder were studied. The key mechanical properties such as compressive strength, tensile strength, and flexural strength of NSCFRC with 0.25% carbon fiber and 1% NS were 6.8%, 20.3%, and 11.7% higher than PC (0% CFs, 0% NS), respectively. Scanning Electron Microscopy (SEM) shows that Nano SiO2 reduced the internal porosity and increased the compactness of the concrete matrix. Furthermore, the experimental result demonstrates that NSCFRC can improve the mechanical properties of concrete after high-temperature and equations were obtained to describe the evolution of residual properties at elevated temperatures. Results suggested that the effect of carbon fibers on the residual properties of concrete after high-temperature is less than steel fiber and polypropylene fiber. It was also indicated that adding appropriate Nano SiO2 to concrete is an effective means to improve the residual performance after high-temperature.
Investigation of Voids Characteristics in an Asphalt Mixture Exposed to Salt Erosion Based on CT Images
Authors: Xiong Rui , Jiang Wenyu , Yang Fa , Li Kehong , Guan Bowen , Zhao Hua
Published: November 2019

The performance of an asphalt mixture will deteriorate under the condition of salt erosion, but there are different opinions on the mechanism of deterioration. Few studies have focused on the relation between the change of void characteristics and performance deterioration of an asphalt mixture exposed to salt erosion. To explore the relation between the air voids characteristics of an asphalt mixture and mechanical damage under salt erosion, the mechanical damage in an asphalt mixture was measured by splitting strength. The asphalt mixture specimens, immersion solutions, asphalt mortar, and aggregate were scanned with CT technology. To segment the voids, the Otsu method was used over asphalt mortar and solution range of CT values. A three-dimensional reconstruction of the CT image was performed with Mimics 20 software to calculate the asphalt mixture’s void characteristics. On this basis, the relationships between the change in void characteristics and splitting strength were analyzed. The results showed that the ideal calculated void fraction can be obtained by threshold segmentation of the image void/asphalt mortar interface with the local CT value Otsu method. Under the salt corrosion environment, the increase of open voids of an asphalt mixture is linearly correlated with the decrease of splitting strength, while salts’ crystallization in the open voids produces crystallization pressure, accelerating the volume growth of open voids. The early damage of an asphalt mixture suffered from the salt may be mainly physical damage. These results can provide a useful reference for the performance of damage research on asphalt mixtures in salt enrichment areas.
Synthesis of Colloidal Au Nanoparticles through Ultrasonic Spray Pyrolysis and Their Use in the Preparation of Polyacrylate-AuNPs’ Composites
Authors: Golub Doris , Ivanič Andrej , Majerič Peter , Tiyyagura Hanuma Reddy , Anžel Ivan , Rudolf Rebeka
Published: November 2019

Colloidal gold nanoparticles (AuNPs) were prepared from two different liquid precursors (gold (III) acetate and gold (III) chloride), using the Ultrasonic Spray Pyrolysis (USP) process. The STEM characterisation showed that the AuNPs from gold chloride are spherical, with average diameters of 57.2 and 69.4 nm, while the AuNPs from gold acetate are ellipsoidal, with average diameters of 84.2 and 134.3 nm, according to Dynamic Light Scattering (DLS) measurements. UV/VIS spectroscopy revealed the maximum absorbance band of AuNPs between 532 and 560 nm, which indicates a stable state. Colloidal AuNPs were used as starting material and were mixed together with acrylic acid (AA) and acrylamide (Am) for the free radical polymerization of polyacrylate-AuNPs’ composites, with the purpose of using them for temporary cavity fillings in the dental industry. SEM characterisation of polyacrylate-AuNPs’ composites revealed a uniform distribution of AuNPs through the polymer matrix, revealing that the AuNPs remained stable during the polymerization process. The density measurements revealed that colloidal AuNPs increase the densities of the prepared polyacrylate-AuNPs’ composites; the densities were increased up to 40% in comparison with the densities of the control samples. A compressive test showed that polyacrylate-AuNPs’ composites exhibited lower compressive strength compared to the control samples, while their toughness increased. At 50% compression deformation some of the samples fracture, suggesting that incorporation of colloidal AuNPs do not improve their compressive strength, but increase their toughness significantly. This increased toughness is the measured property which makes prepared polyacrylate-AuNPs potentially useful in dentistry.
The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells
Authors: Sikora Mateusz , Marcinkowska Klaudia , Marycz Krzysztof , Wiglusz Rafał Jakub , Śmieszek Agnieszka
Published: November 2019

Osteosarcoma (OSA) is malignant bone tumor, occurring in children and adults, characterized by poor prognosis. Despite advances in chemotherapy and surgical techniques, the survival of osteosarcoma patients is not improving significantly. Currently, great efforts are taken to identify novel selective strategies, distinguishing between cancer and normal cells. This includes development of biomimetic scaffolds with anticancer properties that can simultaneously support and modulate proper regeneration of bone tissue. In this study cytotoxicity of scaffolds composed from poly (L-lactic acid) functionalized with nanohydroxyapatite (nHAp) and doped with europium (III) ions—10 wt % 3 mol % Eu3+: nHAp@PLLA was tested using human osteosarcoma cells: U-2 OS, Saos-2 and MG-63. Human adipose tissue-derived stromal cells (HuASCs) were used as non-transformed cells to determine the selective cytotoxicity of the carrier. Analysis included evaluation of cells morphology (confocal/scanning electron microscopy (SEM)), metabolic activity and apoptosis profile in cultures on the scaffolds. Results obtained indicated on high cytotoxicity of scaffolds toward all OSA cell lines, associated with a decrease of cells’ viability, deterioration of metabolic activity and activation of apoptotic factors determined at mRNA and miRNA levels. Simultaneously, the biomaterials did not affect HuASCs’ viability and proliferation rate. Obtained scaffolds showed a bioimaging function, due to functionalization with luminescent europium ions, and thus may find application in theranostics treatment of OSA.
Quantitative Description of External Force Induced Phase Transformation in Silicon–Manganese (Si–Mn) Transformation Induced Plasticity (TRIP) Steels
Authors: He Zhongping , Liu Huachu , Zhu Zhenyu , Zheng Weisen , He Yanlin , Li Lin
Published: November 2019

Transformation Induced Plasticity (TRIP) steels with silicon–manganese (Si–Mn) as the main element have attracted a lot of attention and great interest from steel companies due to their low price, high strength, and high plasticity. Retained austenite is of primary importance as the source of high strength and high plasticity in Si–Mn TRIP steels. In this work, the cold rolled sheets of Si–Mn low carbon steel were treated with TRIP and Dual Phase (DP) treatment respectively. Then, the microstructure and composition of the Si–Mn low carbon steel were observed and tested. The static tensile test of TRIP steel and DP steel was carried out by a CMT5305 electronic universal testing machine. The self-built true stress–strain curve model of TRIP steel was verified. The simulation results were in good agreement with the experimental results. In addition, the phase transformation energy of retained austenite and the work borne by austenite in the sample during static stretching were calculated. The work done by austenite was 14.5 J, which was negligible compared with the total work of 217.8 J. The phase transformation energy absorption of retained austenite in the sample was 9.12 J. The role of retained austenite in TRIP steel is the absorption of excess energy at the key place where the fracture will occur, thereby increasing the elongation, so that the ferrite and bainite in the TRIP steel can absorb energy for a longer time and withstand more energy.
Parametric Study of Flexural Strengthening of Concrete Beams with Prestressed Hybrid Reinforced Polymer
Authors: Wang Xiaomeng , Petrů Michal , Ai Jun , Ou Shikun
Published: November 2019

The strengthening method of using hybrid fiber reinforced polymer is an effective way to increase the strengthening efficiency and lower the cost. This paper focuses on simulating the flexural behavior of reinforced concrete beam strengthened by prestressed C/GFRP (Carbon-Glass hybrid Fiber Reinforced Polymer) with different hybrid ratios and prestress levels. An elastoplastic damage constitution is used to simulate the mechanical behavior of concrete. A cohesive zone model under mixed mode is adopted to describe the debonding behavior of the FRP-concrete and concrete-steel interface. The results show good agreement with the experiment in the load-deflection curve, load-stress curve of steel, and HFRP. Furthermore, the failure mode of concrete and FRP debonding obtained from numerical simulation is the same as the test. Considering the improvement of the bending capacity, stiffness, and ductility of the strengthened beam in this paper, the best hybrid ratio of carbon to glass fiber is 1:1, and the suitable prestress level is between 30 and 50% of its ultimate strength.
Weighted Fractional Iyengar Type Inequalities in the Caputo Direction
Authors: Anastassiou George A.
Published: November 2019

Here we present weighted fractional Iyengar type inequalities with respect to L p norms, with 1 ≤ p ≤ ∞ . Our employed fractional calculus is of Caputo type defined with respect to another function. Our results provide quantitative estimates for the approximation of the Lebesgue–Stieljes integral of a function, based on its values over a finite set of points including at the endpoints of its interval of definition. Our method relies on the right and left generalized fractional Taylor’s formulae. The iterated generalized fractional derivatives case is also studied. We give applications at the end.
Optimal Propagating Fronts Using Hamilton-Jacobi Equations
Authors: Alessandri Angelo , Bagnerini Patrizia , Cianci Roberto , Gaggero Mauro
Published: November 2019

The optimal handling of level sets associated to the solution of Hamilton-Jacobi equations such as the normal flow equation is investigated. The goal is to find the normal velocity minimizing a suitable cost functional that accounts for a desired behavior of level sets over time. Sufficient conditions of optimality are derived that require the solution of a system of nonlinear Hamilton-Jacobi equations. Since finding analytic solutions is difficult in general, the use of numerical methods to obtain approximate solutions is addressed by dealing with some case studies in two and three dimensions.
Stability of the Apollonius Type Additive Functional Equation in Modular Spaces and Fuzzy Banach Spaces
Authors: Kim Sang Og , Michael Rassias John
Published: November 2019

In this work, we investigate the generalized Hyers-Ulam stability of the Apollonius type additive functional equation in modular spaces with or without Δ 2 -conditions. We study the same problem in fuzzy Banach spaces and β -homogeneous Banach spaces. We show the hyperstability of the functional equation associated with the Jordan triple product in fuzzy Banach algebras. The obtained results can be applied to differential and integral equations with kernels of non-power types.
Common Fixed Point Results for Generalized Wardowski Type Contractive Multi-Valued Mappings
Authors: Işık Hüseyin , Parvaneh Vahid , Mohammadi Babak , Altun Ishak
Published: November 2019

In this paper, we introduce generalized Wardowski type quasi-contractions called α - ( φ , Ω ) -contractions for a pair of multi-valued mappings and prove the existence of the common fixed point for such mappings. An illustrative example and an application are given to show the usability of our results.