bluuebunny/crossref_metadata_2025 huggingface dataset:
Oral Tissue Differences Among Conventional And Immediate Dentures
Authors: Angelovska Aneta
Published: January 2025

Background: During the lifetime, each person experiences loss of one or more teeth, which is followed by a series
of problems, such as difficulty in chewing, speaking and perhaps the biggest problem is disturbed aesthetics and
altered appearance.
Conventional and immediate complete dentures including implant-supported total dentures, implant-supported
fixed prostheses are used to rehabilitate completely toothless patients who are often burdened with feelings of
disability, early aging, limited chewing function and reduced mouth space.
The main factors for the acceptance of any mobile prosthetic device are the provision of aesthetic, phonetic,
psycho-social and functional requirements, followed by the fact that patient`s lost aesthetics, instability and
discomfort when wearing dentures are the main factors for dissatisfaction with mobile prosthetic accessories..
Materials and Methods: For realization of the set goals for the research in the Clinic for Removable
Prosthodontics at the Faculty of Dentistry in Skopje, Republic of North Macedonia, during a period of 3 years
60 patients, aged 54 to 70 years, were examined and selected, divided into two groups. In 30 of them, we made
immediate complete dentures, and in the other 30 conventional complete dentures( made over a period of time
from two to six months after tooth extraction).
Results: In our study, we fabricated immediate dentures in patients with an average age of 59 years and 46.7%
of them were with secondary school and 46,7 had higher education, 50% of them had healthy teeth or fixed
prosthetic apliances as antagonists, and conventional dentures were made in patients with an average age of 63.5
years, of which 53.3% had secondary and 23.3% with higher education, and with antagonists predominantly
partial (50%) and complete (36.7%) dentures. In 66.7% of the patients in whom we made immediate dentures,
we did not find disturbed intermaxillary relations, they the height of the lower third of the face were preserved
with the remaining teeth, 73.3% of the subjects in this group had no temporomandibular joint pain. Unlike this,
all patients in the group to whom we made conventional complete dentures had disturbed intermaxillary relations
and 53.3% had temporomandibular joint pain
Conclusion: Patients with immediate dentures have significantly less changes on the orofacial musculature,
fewer have intermaxillary disturbances and extraoral appearance changes, and a smaller number of patients
have temporomandibular joint pain as well as less speech problems, but in these patients the remaining teeth are
usually in very poor periodontal condition. Patients treated with conventional dentures, before the treatment had disturbed extraoral appearance, impaired intermaxillary relations and orofacial musculature with decreased tone, temporomandibular joint pain, and speech difficulties.
Allergic Bronchopulmonary Aspergillosis: A Case Report
Authors: Moumni S. , Khattabi W. El , Mouddene N. Ait , Jabri H. , Afif My.H.
Published: January 2025

Allergic Bronchopulmonary Aspergillosis (ABPA) is an inflammatory disease of the lower airways
characterized by a complex immune response directed against a fungus of the Aspergillus genus. It occurs in
chronic airway diseases such as asthma and cystic fibrosis. It is a rare, severe, and underdiagnosed condition.
This article presents the case of a 56-year-old patient, poorly managed asthmatic for 30 years, with partial
control of symptoms. His medical history dates back 2 months, with a progressive onset of increasingly frequent
and successive asthma attacks, requiring frequent visits to the emergency department for nebulization. The
radiological assessment confirmed the presence of bilateral proximal bronchiectasis. The diagnosis of ABPA
was made according to the 2020 modified ISHAM criteria. Long-term corticosteroid therapy was prescribed under strict monitoring. The outcome showed improvement in dyspnea, asthma control, and a significant improvement in respiratory function
Posterior Reversible Encephalopathy Syndrome As A Rare Manifestation Of Vasculotoxic Snake Bite
Authors: Gupta Dr. Ritu , Singh Dr. Sandeep , Mehra Dr. Rupanshu , Pradhan Dr. Aditya
Published: January 2025

Snake envenomation often leads to various neurological complications but the incidence of leuko-encephalopathy
is quite rare. A 25-year-old male presented following a viperidae snake bite with progressive lower limb swelling.
He was treated with anti-snake venom and hemodialysis but on the fifth day of hospitalization he experienced
multiple episodes of generalised tonic clonic type of seizures. MRI brain revealed features of Posterior reversible
encephalopathy syndrome (PRES). Treatment was mostly supportive and patient was discharged after resolution of clinical symptoms. A repeat MRI after two weeks suggested normal study suggesting radiological resolution. Hence, this case highlights PRES as a possible complication of snake bite.
Rouvier’s Sulcus-Aspects Of Incorporating This Valuable Sign In Safe Laparoscopic Cholecystectomy An Observational Study
Authors: A Dr. Rohit , P Dr.Yashasvi , Kumar Dr P. Anil
Published: January 2025

.
Exploring The Landscape Of Remote Patient Monitoring Systems In India
Authors: . .
Published: January 2025

This study investigates the adoption and perceptions of Remote Patient Monitoring (RPM) systems within the
Indian healthcare landscape, focusing on the perspectives of both pa- tients and healthcare professionals. With a
sample comprising
101 patients from diverse age groups and over 70 healthcare professionals, including doctors, nurses, and medical
students, the research explores the awareness, usage, and feasibility of RPM systems in India. Data were collected
through surveys and interviews, with quantitative analysis employed to sum- marize responses and qualitative
analysis to extract recurring themes. The findings highlight the transformative potential of RPM systems in
enhancing patient care and improving clinical outcomes, while also identifying significant challenges related to
sample size, demographic disparities, and regional variations. Despite these limitations, the study offers valuable
insights into the current state of RPM adoption in India, emphasizing the need for cautious interpretation and
further research to fully understand the complexities of integrating RPM systems across diverse healthcare
settings. This research contributes to the ongoing discourse on the role of RPM in advancing healthcare delivery
in India, providing a foundation for future advancements in this critical area.
Index Terms—Remote Patient Monitoring (RPM), Healthcare technology in India, Telemedicine adoption, Patient
care improve- ment, Clinical outcomes, Healthcare professionals’ perspectives, Healthcare delivery systems,
Indian healthcare landscape, RPM system adoption challenges, Demographic disparities in health- care,
Qualitative and quantitative analysis in healthcare, Sample size limitations in healthcare research, Cultural and regulatory factors in healthcare, Healthcare infrastructure in India, RPM systems in diverse contexts.
Early Excision And Grafting In Burns In A Tertiary Care Centre
Authors: Devi Dr.Divya , P Dr.Catherine Sinduja. , Lankaram Dr.J.J. , Nellaiappar Dr.P.
Published: January 2025

Burns injury continues to be major health burden in the world. Early excision increases survival rate with early
return to daily life.
Aim: to compare morbidity in patients managed by early excision and grafting within 7 days of burns injury and
those patients who were conservatively managed.
Methodology: details of number of surgeries and type of surgery, blood transfusion (units) needed, aba criteria,
length of hospital stay and incidence of sepsis are collected.
Conclusion: early excision and grafting in burns has clear advantages and the treatment ends faster, however due to poor availability of skin substitutes, early excision is restricted to less than 20%. With availability of cadaveric allograft excision can be done upto 30% tbsa burns
Development And Maturation Of Sulci And Gyri Of Cerebral Cortex In Developing Human Foetuses.
Authors: Debbarma Dr Tanusri , Devi Prof & HOD (Dr) N. Damayanti , Singh Prof (Dr) M. Matum
Published: January 2025

Introduction: The surface of the developing fetal brain undergoes significant morphological changes during fetal
growth. The most characteristic feature of the forebrain is its presence of gyri and sulci on its surface and it can
easily be differentiated from midbrain and hindbrain. Different major functional areas of the forebrain are
demarcated by gyri and sulci.
Aims: To study the development and maturation of gyri and sulci of the cerebral in human foetuses.
Objective: To delineate the development of gyri and sulci and maturation of cortical lobules up to term.
Material and Methods: Formalin fixed 85 foetal brains (M- 40, F- 45 ) were dissected at Deptt. of Anatomy,
RIMS, Imphal, after getting formal permission from Institutional Ethics Committee, RIMS, Imphal from the age
group of 12 weeks to 40 weeks and. The results are categorized into five groups: Group I (12- <18weeks), Group
II (19- <24weeks), Group III (24-<30weeks), Group IV (30- <36 weeks) and Group V (36- 40 weeks).
Results: A few chronology of sulcul and gyri development during 12 weeks to 40 weeks were summarized. Before
12 weeks few sulci were present. Measurement of different growth rates linearly increased with GA but no sexual
dimorphism or cerebral asymmetry were detected.
Conclusion: In the youngest specimen of the present study i.e. 12th week of gestation, the external surfaces of cerebral hemispheres were observed smooth i.e Lissencephalic. Features of the right cerebral hemisphere are seen more developed than left.
Plano De Negócios Fácil: Uma Aplicação Web Para Registro E Análise De Informações Empresariais De Pequenos E Médios Empreendimentos
Authors: Naveca Victor Rodrigues , Cabral Kleverton Dos Santos , Kruger Juliano Milton
Published: January 2025

Este artigo apresenta a plataforma “Plano de Negócios Fácil”, uma aplicação web voltada para simplificar e
automatizar o planejamento empresarial, com foco em pequenos e médios empreendedores. O objetivo geral da
pesquisa é oferecer uma solução tecnológica para estruturar e analisar planos de negócios de forma prática e
eficiente. Entre os objetivos específicos destacam-se a criação de uma interface intuitiva, ferramentas de análise
de mercado e projeções financeiras, simulações de cenários e exportação de dados em formatos acessíveis, como
PDF e .docx. A metodologia segue uma abordagem aplicada, exploratório-descritiva e quali-quantitativa,
utilizando frameworks como Laravel, React, e Tailwind para o desenvolvimento. O banco de dados MySQL e o
modelo de inteligência artificial generativa Gemini 1.5 foram integrados para realizar análises detalhadas, como
SWOT e viabilidade financeira, permitindo feedback personalizado e suporte estratégico aos usuários. Os
resultados incluem a criação de uma plataforma robusta, com funcionalidades avançadas que abrangem desde
a análise de mercado até a geração de relatórios financeiros automatizados. A aplicação foi validada com
usuários de diferentes níveis de experiência, demonstrando impacto positivo na redução de erros comuns e
aumento da confiança dos empreendedores. Conclui-se que a plataforma atende a uma lacuna no mercado ao democratizar o acesso a soluções de planejamento estratégico, proporcionando suporte abrangente e reduzindo os riscos de novos empreendimentos.
Implication Des Gestantes A La Consultation Prénatale A La Seizième Semaine En Zone De Santé d’Oicha /RD Congo
Authors: Pascal Bailanda Mumbere , Précieuse Vivuya Deu , Anaclet Isse Mighambo , Trésor Mosomo , Mulagha Kambale
Published: January 2025

La grossesse n’est pas une maladie, elle évolue et se termine sur un mode physiologique dans la majorité des cas,
tout en apportant de la joie dans la famille. Elle peut aussi apporter la tristesse soit le décès du nouveau-né, de
la mère ou les deux à la fois.
La CPN sert à détecter et confirmer la grossesse, surveiller la bonne évolution de la grossesse, détecter et traiter
les nuisances, les éventuelles complications de la grossesse et de donner des conseils sur l’accouchement,
l’allaitement et l’espacement des naissances.
Notre recherche centrée sur la consultation prénatale vise d’évaluer l’implication des gestantes à la consultation
prénatale à la seizième semaine dans la zone de santé d’Oicha.
Notre étude a été menée dans la zone de santé d’Oicha, en République Démocratique du Congo. Une étude
transversale analytique menée sur 400 enquêtées sur la période allant du 1er au 30 Juin 2024. L’interview et le
questionnaire ont été utilisés.
Ainsi, les consultations prénatales constituent l’un des quatre piliers de la maternité à moindre risque.
L’âge des gestantes 14 - 19 ans p-value de 0,023 ; 20- 24 ans p-value de 0,034 ; 25 - 29 ans p-value de 0,042 ;
30 - 34 ans P-value de 0,031 et 35 - 39 ans p-value de 0,029). Manque de connaissance de la CPN p-value de
0,001 ; manque d’information sur le plan d’accouchement avec p-value de 0,033 ; sous information de
l’avantage de la CPN avec p-value de 0,000 ; Sous information du nombre de CPN avec p-value de 0,027 :
Developing Reading Competence For Third And Fourth Form Pupils Of Nutrition Option In Mutwanga And Bulongo Counties/Rd. Congo
Authors: Ndaleana Kakule , Joël Kambale Isevahani
Published: January 2025

Dans ce travail qui a comme étude de base le développement des compétences en lecture des élèves de 3
e
et 4e
Année de Nutrition à Mutwanga et Bulongo, comme l'un des problèmes auxquels sont habituellement confrontés
nos apprenants. Nous avons été motivé pour rédiger cet article qui est utile à nos lecteurs et à d'autres
chercheurs, car il contient le matériel nécessaire.
S'interrogeant sur l'échec des apprenants à lire certains documents rédigés en anglais, nous avons enquêté sur
certaines écoles organisant l'option nutrition. Le résultat de notre enquête, après interprétation, nous permet de
confirmer que sur un total de 120 élèves enquêtés, 13 élèves soit 10,8% se sont bien remarqués dans la lecture.
Les responsabilités des échecs des élèves en lecture étant multidimensionnelles, nous souhaitons, à travers ce
présent article, donner des suggestions pouvant conduire à de bons résultats attendus. A travers cette
recherche, nous avons découverts que les enseignants d'anglais dans les écoles organisant l'option Nutrition
qui utilisent des techniques stéréotypées qui ne forment pas les élèves à lire couramment, doivent changer les
attitudes en adoptant des principes et des techniques de communication dans l'enseignement des cours de
lecture, qui mettent l'accent sur les interactions en classe.
Ensuite ils doivent être bien équipés en documentation liés à l'option Nutrition, à partir desquels ils doivent
sélectionner des matériaux qui attireront l'attention et l'intérêt des élèves pour la lecture.Ils doivent améliorer
les bâtiments scolaires afin d'être confortables, ce qui permet un bon apprentissage des élèves pendant le cours
de lecture. Quant aux responsables des écoles ils doivent aussi doter leurs écoles par des documents liés à
l’option NUTRITION et mettre à disposition des élèves des textbooks pour leurs exercices d'amélioration de
leurs lectures et fournir beaucoup d'efforts en assistant les enseignants pendant les exercices de lecture
(s'intéresser à la lecture et éviter la paresse) car la pratique rend parfait. Enfin ils doivent créer une opportunité illimitée pour les apprenants de développer leur lecture, en créant des bibliothèques privées dans lesquelles les documents de l'option Nutrition doivent être représentés sur les rayons
What Psychological Factors Influence Consumer Decision-Making In The Context Of Pricing Strategies?
Authors: Modi Mohit
Published: January 2025

.
Investigating The Effect Of Cultural Influences And Consumer Behaviour On Product Branding In The Manufacturing Sector In Ghana
Authors: Takyi Samuel Akomea , Owusu Jeanette , Korsah Lucy Adubea , Boateng Peter Agyekum , Owusu-Yeboah Ebenezer
Published: January 2025

This study investigates the effects of cultural influences and consumer behavior on product branding within the
manufacturing sector in Ghana. By employing a comprehensive literature review and thematic analysis, the
research identifies how cultural values, beliefs, and practices shape consumer preferences and purchasing
decisions. Key findings reveal that communal living and collective decision-making significantly influence
consumer engagement with brands. Effective branding strategies must align with these cultural values,
incorporating local languages, cultural symbols, and community-oriented benefits to enhance trust and loyalty.
The study also highlights the challenges faced in branding, such as language barriers, diverse consumption
habits, and varied levels of consumer education, necessitating differentiated marketing strategies for urban and
rural consumers. The research contributes to the understanding of how Hofstede’s Cultural Dimensions Theory
and the Customer-Based Brand Equity (CBBE) model apply in Ghana’s manufacturing sector. It provides
actionable insights for practitioners, emphasizing the importance of culturally sensitive branding strategies, the
use of digital platforms for real-time consumer engagement, and the role of brand authenticity in building consumer trust. Future research should explore the impact of urbanization and technological advancements on consumer behavior and branding strategies in Ghana.
Dynamics Of Federalism And Impacts On State Capacity: A Scientometric Analysis
Authors: De Oliveira, Laize Almeida , Do Prado, José Willer
Published: January 2025

Background: With the promulgation of the 1988 Constitution, the promotion of municipal state capacities became
a central theme associated with federalism. There has been an increase in scientific production in recent decades
regarding the federative perspective and its impacts on municipal state capacities in Brazil. The objective of this
study was to investigate and analyze the intellectual structure of studies that relate state capacity and federalism,
identifying their constitutive elements and the influences they exert on the understanding of the topic during the
period from 1999 to 2023.
Materials and Methods: The study has a descriptive and exploratory nature with a quantitative-qualitative
approach. For data processing, the scientometric technique was used with the aid of the VOSviewer software.
The search was conducted on March 27, 2023, retrieving 279 articles through title, abstract, and keyword
searches in the Web of Science. Filters were applied to refine the search, selecting “Article” and “Open Access,”
resulting in 76 articles. After an initial screening, a final sample of 72 articles was established.
Results: The peak of publications and citations occurred during the COVID-19 pandemic, suggesting a growing
concern with understanding how federalism can affect governments' capacity to address health issues and the
importance of the state's role in this context.
Conclusion: Its importance stands out in the decentralization of responsibilities, the promotion of state capacity, and the response to institutional crises. This concern is not limited to Brazil, the United States, and China; it extends to other countries, highlighting that it is a global issue of great relevance.
Educação A Distância No Brasil: Análise Estatística Das Desigualdades Regionais E Institucionais Na Produção Científica (1994-2003)
Authors: Vasconcelos Eduardo Silva , Vasconcelos Leia Rocha
Published: January 2025

Este estudo analisa a produção científica sobre Educação a Distância (EAD) nos programas de pós-graduação
stricto sensu no Brasil entre 1994 e 2003, com foco nas desigualdades regionais e institucionais. O problema
central investiga como essas desigualdades influenciam a consolidação e expansão da EAD e identifica
estratégias para promover maior equidade e diversidade temática. O objetivo é compreender a concentração
geográfica e temática das dissertações e teses e propor recomendações para descentralizar recursos e ampliar
a interdisciplinaridade. A pesquisa utiliza metodologia bibliográfica e quantitativa, analisando dados de bases
acadêmicas e do portal CAPES. A análise revelou que 84% da produção científica concentra-se nas regiões
Sudeste e Sul, enquanto as regiões Norte e Nordeste apresentam participação mínima. Em termos institucionais,
a Universidade Federal de Santa Catarina (UFSC) lidera as contribuições, com destaque para a Engenharia de
Produção. As áreas predominantes incluem Educação e Ciências Humanas, evidenciando o impacto pedagógico
e tecnológico da modalidade. O estudo destaca a necessidade de políticas públicas que incentivem a pesquisa em regiões menos representadas, promovendo uma distribuição mais equitativa e inovadora da EAD no Brasil.
Evaluation of Assisted Reproductive Technology Health Insurance Coverage for Multiple Pregnancies and Births in Korea
Authors: Cha Wontae , Yun Il , Nam Chung-Mo , Nam Jin Young , Park Eun-Cheol
Published: June 2023

Importance

While various policies to support couples experiencing infertility have been introduced due to the fertility rate rapidly dropping in developed countries, few large-scale nationwide cohort studies have evaluated the outcomes of assisted reproductive technology (ART) health insurance coverage policies.

Objective

To evaluate ART health insurance coverage for multiple pregnancies and births in Korea.

Design, Setting, and Participants

This population-based cohort study used delivery cohort data from the Korean National Health Insurance Service database between July 1, 2015, and December 31, 2019. A total of 1 474 484 women were included after exclusion of those who gave birth at nonmedical institutions and those with missing data.

Exposure

Two 27-month periods were examined before and after the Korean National Health Insurance Service had begun covering ART treatment (preintervention period, July 1, 2015, to September 30, 2017; postintervention period, October 1, 2017, to December 31, 2019).

Main Outcomes and Measures

Multiple pregnancies and multiple births were identified by International Statistical Classification of Diseases and Related Health Problems, Tenth Revision diagnosis codes. Total births were defined as the total number of babies born to each pregnant woman during the follow-up period. An interrupted time series with segmented regression was conducted to analyze the time trend and its change in outcomes. Data analysis was conducted between December 2, 2022, and February 15, 2023.

Results

Of the 1 474 484 women eligible for the analysis (mean [SD] age, 33.2 [4.6] years), approximately 1.60% had multiple pregnancies and 1.10% had multiple births. After covering ART treatment, the likelihood of multiple pregnancies and multiple births was estimated to increase by 0.7% (estimate, 1.007; 95% CI, 1.004-1.011; P < .001) and 1.2% (estimate, 1.012; 95% CI, 1.007-1.016; P < .001) compared with before coverage. The probability of an increase in the number of total births per pregnant woman after the intervention was estimated to be 0.5% (estimate, 1.005; 95% CI, 1.005-1.005; P < .001). The relatively high-income class above the median income showed a decreasing trend in multiple births and total births before the intervention, but after the intervention, a significant increase was observed.

Conclusions and Relevance

This population-based cohort study found that the possibility of multiple pregnancies and births in Korea significantly increased after the implementation of an ART health insurance coverage policy. These findings suggest that the development and coverage of policies to support couples experiencing infertility may help address low fertility rates.
Cell Electrospinning: a Review of Materials and Methodologies for Biofabrication
Authors: Maurmann Natasha , França Fernanda S. , Girón Juliana , Pranke Patricia
Published: June 2023

The process of electrohydrodynamic living cell microencapsulation inside a scaffold during the electrospinning (ES) process is called cell electrospinning (CE). Several studies demonstrate the feasibility of using cell electrospinning for biomedical applications, allowing for the direct biofabrication of living cells to be encapsulated in fibers for the formation of active biological scaffolds. In this review, a comprehensive overview of the materials and methodologies used in cell electrospinning, as well as their biomedical application in tissue engineering, is provided. Cell ES represents an innovative technique for automated application in regenerative medicine.
Intracellular Liquid‐Liquid Phase Separation Induces Tunable Anisotropic Nanocrystal Growth for Multidimensional Analysis
Authors: Liu Hao , Liu Zhiming , Wang Yihan , Xiao Jiang , Liu Xiaohui , Jiang Hui , Wang Xuemei
Published: June 2023

Spatially directed biomineralization of nanocrystals for specific optical purposes in biological systems and elucidation of the mechanism of biomineralized nanocrystals remains a fascinating but extremely challenging task. Herein, the biomineralization of tunable gold nanocrystals with in situ protein coronas by intracellular liquid‐liquid phase separation (LLPS) for in situ molecule surface‐enhanced Raman spectrum (SERS) analysis and tumor fluorescence identification. The anisotropic nanocrystals are realized by regulating LLPS, in which the nucleation of nanocrystals is at the protein secondary structural site (β‐Corner) of the droplet, the different adsorption energies of crystal surfaces further promote the directional growth of nanocrystals. Interestingly, gold nanocrystals with ultra‐narrow fluorescence emission at half‐peak width and ultra‐long Stokes shift and can obtain in situ molecular fingerprint information of protein corona to achieve by SERS. Of note are the anisotropic nanocrystals to differentiate clinical patient tumor tissue samples. Therefore, this study reveals the mechanism of intracellular tunable nanocrystal biomineralization and provides the basis for its biomedical application.
Metallic Particles‐Induced Surface Reconstruction Enabling Highly Durable Zinc Metal Anode
Authors: Liu Wen , Zhao Qiwen , Yu Huaming , Wang Han , Huang Shaozhen , Zhou Liangjun , Wei Weifeng , Zhang Qichun , Ji Xiaobo , Chen Yuejiao , Chen Libao
Published: June 2023

Aqueous zinc batteries usher in a renaissance due to their intrinsic security and cost effectiveness, bespeaking vast application foreground for large‐scale energy storage system. However, uncontrolled dendrite growth along with hydrogen evolution severely restricts its reversibility and stability for practical application. Herein, the surface of Zn metal is reconstructed with metallic particles (In, Sn, In0.2Sn0.8) to diminish surface defects and regulate Zn deposition behavior. The alloyed In–Sn greatly activates the Zn surface for lower Zn adsorption energy barrier to expedite plating kinetics and confine Zn aggregation. Dense and uniform deposition of Zn on the reconstructed surface significantly prevents the Zn substrate from dendrites growth for catastrophic damage. Meanwhile, alloy layer embodies high hydrogen evolution overpotential, ensuring high plating and stripping efficiency for Zn anode. Consequently, In0.2Sn0.8 reconstructed surface realizes long‐term lifespan up to 1800 h with low polarization (12 mV) at the condition of 1 mA cm−2 and 1 mAh cm−2. When paired with sodium vanadate (NVO) cathode, the full cell steady operates for a high‐capacity retention of 94.0% after 5000 cycles at 5 A g−1. This study provides new insights into the surface‐defects dependent Zn deposition process and offers a guide for constructing stable surface for dendrite‐free Zn growth.
From Aluminum Dissolution in Supercapacitors to Electroplating: A New Way for Al Thin Film Deposition?
Authors: Zaccagnini Pietro , Heß Lars Henning , Baudino Luisa , Laurenti Marco , Serrapede Mara , Lamberti Andrea , Balducci Andrea
Published: June 2023

The present work addresses a new finding observed while performing aluminum dissolution experiments for supercapacitors (SCs) stability investigation. Supercapacitor (SC) electrodes based on carbon‐coated aluminum foils are electrochemically cycled in harsh conditions into bis‐trifluoromethylsulfonyl imide (TFSI)‐based electrolyte and using Acetonitrile (ACN) as solvent. Dissolution of aluminum is observed with subsequent plating on the carbonaceous surface of counter electrodes. Moreover, the same process can be reproduced also on standard SC activated carbon electrodes. This mechanism can open the way to an effective strategy to achieve Al film deposition by electroplating becoming competitive with the most common copper counterpart.
Inverse metabolic engineering for improving protein content in Saccharomyces cerevisiae
Authors: Lee Young‐Oh , Do Sang‐Hun , Won Jae Yoon , Chin Young‐Wook , Chewaka Legesse S. , Park Bo‐Ram , Kim Soo‐Jung , Kim Sun‐Ki
Published: June 2023

Production of Saccharomyces cerevisiae‐based single cell protein (SCP) has recently received great attention due to the steady increase in the world's population and environmental issues. In this study, an inverse metabolic engineering approach was applied to improve the production of yeast SCP. Specifically, an S. cerevisiae mutant library, generated using UV‐random mutagenesis, was screened for three rounds to isolate mutants with improved protein content and/or concentration. The #1021 mutant strain exhibited a respective 31% and 23% higher amino acid content and concentration than the parental S. cerevisiae D452‐2 strain. Notably, the content, concentration, and composition of amino acids produced by the PAN2* strain, with a single nucleotide polymorphism in PAN2 coding for a catalytic subunit of the poly(A)‐nuclease (PAN) deadenylation complex, were virtually identical to those produced by the #1021 mutant strain. In a glucose‐limited fed‐batch fermentation, the PAN2* strain produced 19.5 g L−1 amino acids in 89 h, which was 16% higher than that produced by the parental D452‐2 strain. This study highlights the benefits of inverse metabolic engineering for enhancing the production titer and yield of target molecules without prior knowledge of rate‐limiting steps involved in their biosynthetic pathways.
Structural Optimization, Fungicidal Activities Evaluation, DFT Study and Structure‐Activity Relationship of Dopamine Derivatives with Benzothiazole Fragment from Polyrhachis dives
Authors: Wang Yaxuan , Huang Danling , Cheng Yong‐Xian
Published: June 2023

In our previous work, two dopamine derivatives with benzothiazole fragment were isolated and identified from Polyrhachis dives (P. dives). Based on their characteristic structure, we used them as lead compound to carry out structural optimization and subsequent fungicidal evaluation. Here 20 dopamine derivatives with benzothiazole fragment were designed and synthesized by a facile method, and their structures were characterized by 1H‐NMR, 13CNMR and HMRS. In bioassays, most of the title compounds possess potential fungicidal activities against Altenaia alternala (A. alternala) and Botrytis cinerea (B. cinerea). Especially, (E)‐N‐(2‐(benzo[d]thiazol‐6‐yl)ethyl)‐3‐(p‐tolyl)acrylamide and (E)‐N‐(2‐(benzo[d]thiazol‐6‐yl)ethyl)‐3‐(4‐(trifluoromethyl)phenyl)acrylamide displayed 29.3 mg/L and 10.7 mg/L EC50 value against A. alternala, respectively, which possessed equivalent fungicidal activities level to hymexazol.
Effect of plasmid DNA isoforms on preparative anion exchange chromatography
Authors: Kralj Špela , Kodermac Špela Meta , Bergoč Ines , Kostelec Tomas , Podgornik Aleš , Štrancar Aleš , Černigoj Urh
Published: June 2023

Increased need for plasmid DNA (pDNA) with sizes above 10 kbp (large pDNA) in gene therapy and vaccination brings the need for its large‐scale production with high purity. Chromatographic purification of large pDNA is often challenging due to low process yields and column clogging, especially using anion‐exchanging columns. The goal of our investigation was to evaluate the mass balance and pDNA isoform composition at column outlet for plasmids of different sizes in combination with weak anion exchange (AEX) monolith columns of varying channel size (2, 3 and 6 µm channel size). We have proven that open circular pDNA (OC pDNA) isoform is an important driver of reduced chromatographic performance in AEX chromatography. The main reason for the behaviour is the entrapment of OC pDNA in chromatographic supports with smaller channel sizes. Entrapment of individual isoforms was characterised for porous beads and convective monolithic columns. Convective entrapment of OC pDNA isoform was confirmed on both types of stationary phases. Porous beads in addition showed a reduced recovery of supercoiled pDNA (on an 11.6 kbp plasmid) caused by diffusional entrapment within the porous structure. Use of convective AEX monoliths or membranes with channel diameter >3.5 µm has been shown to increase yields and prevent irreversible pressure build‐up and column clogging during purification of plasmids at least up to 16 kbp in size.
Barriers in quest for cancer care access in two states of northeast India
Authors: Kuru Dindi , Kumar K Anil , Chaturvedi Pankaj
Published: June 2023

Background

The cancer burden in northeast India is high, with low survival and low case detection. Despite the availability of cancer institutes in the region, existing literature remarks on the increasing travel outside the region for cancer care. However, research is sparse on identifying impediment factors to the access of state cancer institutes.

Objective

To examine the barriers to cancer care in five common cancer sites: oral, lungs, stomach, breast and cervix.

Method

Following a descriptive multiple‐embedded case study design integrating quantitative and qualitative approaches, 388 participants were selected in phase one by stratified random sampling. In phase two, by purposive sampling, 21 semi‐structured interviews were conducted.

Result

The result suggests that family decision is the central factor in cancer care access. Treatment initiation is delayed because the existing government health insurance scheme does not cover diagnostic tests. Adverse steps are taken to fund cancer treatment. Besides, opting for alternative medicines were due to fear of surgery, chemotherapy and recommendations by relatives. Arranging accommodation, transportation and infrastructure shortage was another hurdle. In contrast, the lack of awareness of the state cancer institutes was a barrier to its access.

Conclusion

This paper identifies and describes factors that hinder access to state cancer institutes. The findings could enhance policy interventions for efficient cancer care access in the region. Integration with NGOs working at the state level for cancer services would support ease of access by providing funds for diagnostic tests, accommodation and transportation, especially for those who cannot afford it.
The offshore wind energy potential of Morocco: Optimal locations, cost analysis, and socioenvironmental examination
Authors: Charouif Yassine , Lehnert Matthew R.
Published: June 2023

Morocco, a country without substantial fossil fuels, is emerging as a leader in renewable energy. This article addresses an untapped natural resource in Morocco: offshore wind. Methodologically, we use geographic information systems to assess optimal locations for offshore wind energy plants based on wind speed and power density from the Global Wind Atlas. We incorporate geological, environmental, and economic parameters and calculate capital expenses, operating expenses, development costs, and decommissioning costs to determine if the wind plant will provide a return on investment during its lifetime. The results identify three locations near the cities of Dakhla, Boujdour, and Essaouira that would recoup their investment in 14, 13, and 18 years, respectively, along with supplying 21.8% of Morocco's current energy needs for 2022. We conclude by considering the implications that the new plants would have for Morocco in the social, political, environmental, and economic dimensions. Integr Environ Assess Manag 2024;20:201–210. © 2023 SETAC
The consequences of neglected confounding and interactions in mixed‐effects meta‐regression: An illustrative example
Authors: Knop Eric S. , Pauly Markus , Friede Tim , Welz Thilo
Published: June 2023

Analysts seldom include interaction terms in their meta‐regression model, which can introduce bias if an interaction is present. We illustrate this by reanalysing a meta‐regression study in acute heart failure. Based on a total of 285 studies, the 1‐year mortality rate related to acute heart failure is considered and the connection to the study‐level covariates year of recruitment and average age of study participants are of interest. We show that neglecting a possibly confounding variable and an interaction term might lead to erroneous inference and conclusions. Based on our results and accompanying simulations, we recommend to include possible confounders and interaction terms, whenever they are plausible, in mixed‐effects meta‐regression models.
Pesticide residues in herbs and their transfer for infusions
Authors: Caldeirão Lucas , Godoy Helena Teixeira , Fernandes José , Cunha Sara C.
Published: June 2023

In this work, a cost‐effective gas chromatography‐mass spectrometry method was validated for the analysis of twenty‐five pesticide residues in herbs and infusions using a quick, easy, cheap, effective, rugged, and safe procedure or a dispersive liquid‐liquid microextraction method, respectively. Figures of merit of the method showed good accordance with current guidelines. From the 58 herb samples studied (pertaining to 20 different species), 80% presented at least one detectable pesticide, with 62% of them above the maximum residual level. Results showed that pesticide residues from naturally contaminated herbs were not transferred at a significant rate to the herbal infusions. When a control assay was conducted by spiking a blank herb sample with a large amount of each pesticide (7 mg/L) 15 analytes were detected below the limit of quantification in the infusion.
Research on the relationship between defense technology innovation and high‐quality economic development: Gray correlation analysis based on panel data
Authors: Cai Daran , Hu Jin , Jiang Hongtao , Ai Fengyi , Bai Tingting
Published: June 2023

Based on panel data from 2010 to 2019, this study examines the relationship between defense science and technology innovation and high‐quality economic development at the provincial level in China. Using the gray correlation analysis method of panel data and an evaluation index system of high‐quality economic development, we calculate the gray correlation coefficient and decompose its time‐series gray correlation degree and cross‐sectional. Our findings indicate that the correlation between defense science and technology innovation and high‐quality economic development in China is generally stable during 2010–2019. However, Beijing stands out as an exception with a correlation coefficient of less than 0.5. Furthermore, there is regional gray correlation heterogeneity with the smallest correlation degree found in the eastern region and the largest in the northeast region. Based on our results, we propose three countermeasure suggestions: first, to balance national defense construction and economic construction; second, to optimize national defense science and technology strategy according to local conditions; and third, to effectively utilize industrial structure advantages. In summary, our study contributes to the literature by highlighting the relationship between defense science and technology innovation and high‐quality economic development in China. Our findings provide implications for policymakers and practitioners to make informed decisions in achieving sustainable and high‐quality economic development.
How much food tracking during a digital weight‐management program is enough to produce clinically significant weight loss?
Authors: Xu Ran , Bannor Richard , Cardel Michelle I. , Foster Gary D. , Pagoto Sherry
Published: June 2023

Objective

The aim of this study was to identify the levels of food‐tracking adherence that best predict ≥3%, ≥5%, and ≥10% weight loss at 6 months and to identify distinctive food‐tracking trajectories among participants in a 6‐month, commercial digital weight‐management program.

Methods

This study used data from 153 participants of a single‐arm trial of a commercial digital weight‐management program, WeightWatchers (WW). Receiver operating characteristic (ROC) curve analysis was conducted to identify the optimum thresholds of food tracking that can predict ≥3%, ≥5%, and ≥10% weight loss at 6 months. Time series clustering analysis was performed on weekly food‐tracking data to identify trajectories and compare trajectories on weight loss at 6 months.

Results

ROC analyses showed that the optimum thresholds of food tracking were 28.5% of the intervention days to achieve ≥3% weight loss (area under the curve [AUC] = 0.820, p < 0.001), 39.4% to achieve ≥5% weight loss (AUC = 0.744, p < 0.001), and 67.1% to achieve 10% weight loss (AUC = 0.712, p = 0.002). Time series clustering analyses found three food‐tracking trajectories. Trajectories differed significantly in weight loss at 6 months (F = 14.1, p < 0.001).

Conclusions

Results suggest that perfect food‐tracking adherence is not necessary to achieve clinically significant weight loss.
An N‐glycopeptide MS/MS data analysis workflow leveraging two complementary glycoproteomic software tools for more confident identification and assignments
Authors: Kuo Chu‐Wei , Chang Ning‐En , Yu Pei‐Yu , Yang Tzu‐Jing , Hsu Shang‐Te Danny , Khoo Kay‐Hooi
Published: June 2023

Complete coverage of all N‐glycosylation sites on the SARS‐CoV2 spike protein would require the use of multiple proteases in addition to trypsin. Subsequent identification of the resulting glycopeptides by searching against database often introduces assignment errors due to similar mass differences between different permutations of amino acids and glycosyl residues. By manually interpreting the individual MS2 spectra, we report here the common sources of errors in assignment, especially those introduced by the use of chymotrypsin. We show that by applying a stringent threshold of acceptance, erroneous assignment by the commonly used Byonic software can be controlled within 15%, which can be reduced further if only those also confidently identified by a different search engine, pGlyco3, were considered. A representative site‐specific N‐glycosylation pattern could be constructed based on quantifying only the overlapping subset of N‐glycopeptides identified at higher confidence. Applying the two complimentary glycoproteomic software in a concerted data analysis workflow, we found and confirmed that glycosylation at several sites of an unstable Omicron spike protein differed significantly from those of the stable trimeric product of the parental D614G variant.
Discovery of phosphonate derivatives containing different substituted 1,2,3‐triazole motif as promising tobacco mosaic virus (TMV) helicase inhibitors for controlling plant viral diseases
Authors: Li Zhenxing , Yang Binxin , Ding Yue , Zhou Xiang , Fang Zimian , Liu ShuaiShuai , Yang Jie , Yang Song
Published: June 2023

Background

The discovery and identification of targets is of far‐reaching significance for developing novel pesticide candidates and increasing the probability of success. To explore and identify highly effective tobacco mosaic virus (TMV) helicase‐targeted lead structures, a series of novel phosphonate derivatives containing a 1,2,3‐triazole motif were rationally engineered and their antiviral activity was assessed.

Results

Bioassay results showed that the optimized B17 exhibited more potent curative activity (EC50 = 271.5 μg mL−1) against TMV in vivo, which was superior to that of commercial Ribavirin (EC50 = 689.3 μg mL−1). B17 presented a stronger binding capacity through binding analysis with helicase, affording a corresponding value of 12.7 μM. Enzyme activity assay showed B17 exhibited excellent inhibitory activity on TMV helicase (39.2% at 300 μM). Furthermore, molecular docking simulations demonstrated that B17 displayed strong hydrogen‐bond interactions (2.1, 2.1, 2.2, and 3.2 Å) with Ala‐33, Gly‐10, Gly‐8, and Glu‐217 of TMV helicase. Encouragingly, transmission electron microscopy analysis revealed that B17 could remarkably disrupt surface morphology and inhibit TMV proliferation. Additionally, these compounds also displayed potential anti‐CMV (cucumber mosaic virus) and antipathogens (Xanthomonas oryzae pv. oryzae and Xanthomonas axonopodis pv. citri) by expanding their applications in agriculture.

Conclusion

Current research demonstrated that B17 could be considered as a potential antiviral agent alternative though targeting TMV helicase. © 2023 Society of Chemical Industry.
Augmented reality simulation as training model of ventricular puncture: Evidence in the improvement of the quality of punctures
Authors: Domínguez‐Velasco César F. , Tello‐Mata Isaac E. , Guinto‐Nishimura Gerardo , Martínez‐Hernández Adriana , Alcocer‐Barradas Víctor , Pérez‐Lomelí Juan S. , Padilla‐Castañeda Miguel A.
Published: June 2023

Background

Ventricular puncture is a common procedure in neurosurgery and the first that resident must learn. Ongoing education is critical to improving patient outcomes. However, training at the expense of potential risk to patients warrants new and safer training methods for residents.

Methods

An augmented reality (AR) simulator for the practice of ventricular punctures was designed. It consists of a navigation system with a virtual 3D projection of the anatomy over a 3D‐printed patient model. Forty‐eight participants from neurosurgery staff performed two free‐hand ventricular punctures before and after a training session.

Results

Participants achieved enhanced accuracy in reaching the target at the Monro foramen after practicing with the system. Additional metrics revealed significantly better trajectories after the training.

Conclusion

The study confirms the feasibility of AR as a training tool. This motivates future work towards standardising new educative methodologies in neurosurgery.
Language Report Dutch
Authors: Steurs Frieda , Vandeghinste Vincent , Daelemans Walter
Published: June 2023

This chapter provides a new state of affairs (Steurs et al. 2022) with regard to language technology (LT) for Dutch (after Odijk 2012). LT for Dutch is highly developed, and the Netherlands and Flanders have a strong and cooperative LT community. A lot of digital data is freely available through CLARIN and the Dutch Language Institute (INT). However, data and software have to be updated continuously, and there is a need for a new overarching programme to support research initiatives.
Language Report Finnish
Authors: Lindén Krister , Dyster Wilhelmina
Published: June 2023

During the last ten years, digitalisation has changed the way we interact with the world creating an increasing demand for language-based AI services. In the field of language technology, the Finnish language is still only moderately equipped with products, technologies and resources. The situation has improved in recent years, but still support for automated translation leaves room for ample improvement, as the general support for spoken language is modest in industry applications although some recent research results are encouraging. We take stock of the existing resources for Finnish and try to identify some remaining gaps.
Language Report Galician
Authors: Sánchez José Manuel Ramírez , Fernández Laura Docío , García-Mateo Carmen
Published: June 2023

This chapter reports on the current state of Language Technology (LT) for Galician. The main conclusion is that there are a limited number of resources, products, and technologies for the Galician language with text-based technologies and services being more mature than those based on speech processing. We start with general facts about Galician, followed by a high-level qualitative description of the LT situation for Galician, and conclude with recommendations for bridging the gap between Galician LT with Spanish and the other co-official languages of Spain.