bluuebunny/crossref_metadata_2025 huggingface dataset:
Microtopography and Barrier Function in Healthy Skin: Differences between Forearm, Cheek and Palm
Authors: Sanabria-de la Torre Raquel , Ceres-Muñoz María , Pretel-Lara Carlota , Montero-Vílchez Trinidad , Arias-Santiago Salvador
Published: December 2023

(1) Background: Skin barrier function resides mostly in the stratum corneum, which consists of a protein component, the corneocyte (bricks), which provides a scaffold for the second component, the extracellular matrix, consisting of multilayers of lipids (mortar). These two components closely interact and this could be the basis for the differences in the biophysical properties of the skin between anatomical regions. So, the aim of this study was to compare skin microstructural properties between body sites. (2) Methods: A comparative study was conducted that included healthy individuals without previous skin diseases. Skin barrier function parameters and microtopography parameters (smoothness, roughness, desquamation, wrinkles, surface, volume, contrast, variance, homogeneity, anisotropy, total cell count, flaking index, skin surface hardness, brightness, deformability and friction) were measured on the forearm, cheek and palm. (3) Results: 44 participants were included in this study, with a mean age of 38.8 ± 15.0 years. Significant differences were found between body sites for 14 of the 15 parameters evaluated. Smoothness was higher on the forearm than on the cheek and palm (240.02 Sems vs. 348.16 vs. 408.19 Sems, p < 0.05). Hardness was higher on the palm than on the forearm and cheek (13.22 AU vs. 9.44 AU vs. 7.94 AU, p < 0.05). Moreover, we observed that sociodemographic characteristics such as age, sex, tobacco and/or alcohol use, influenced the parameters evaluated. (4) Conclusions: The differences in skin barrier function and microtopography between anatomical regions reflects the different structure of skin in each body part and could help to understand the influence of the sociodemographic characteristics on theses parameters. This information could be useful for comparison with pathological skin characteristics and for targeting new treatments.
DNA Methylome and Transcriptome Maps of Primary Colorectal Cancer and Matched Liver Metastasis
Authors: Ajithkumar Priyadarshana , Gimenez Gregory , Stockwell Peter A. , Almomani Suzan , Bowden Sarah A. , Leichter Anna L. , Ahn Antonio , Pattison Sharon , Schmeier Sebastian , Frizelle Frank A. , Eccles Michael R. , Purcell Rachel V. , Rodger Euan J. , Chatterjee Aniruddha
Published: December 2023

Sequencing-based genome-wide DNA methylation, gene expression studies and associated data on paired colorectal cancer (CRC) primary and liver metastasis are very limited. We have profiled the DNA methylome and transcriptome of matched primary CRC and liver metastasis samples from the same patients. Genome-scale methylation and expression levels were examined using Reduced Representation Bisulfite Sequencing (RRBS) and RNA-Seq, respectively. To investigate DNA methylation and expression patterns, we generated a total of 1.01 × 109 RRBS reads and 4.38 × 108 RNA-Seq reads from the matched cancer tissues. Here, we describe in detail the sample features, experimental design, methods and bioinformatic pipeline for these epigenetic data. We demonstrate the quality of both the samples and sequence data obtained from the paired samples. The sequencing data obtained from this study will serve as a valuable resource for studying underlying mechanisms of distant metastasis and the utility of epigenetic profiles in cancer metastasis.
Defect Isolation from Whole to Local Field Separation in Complex Interferometry Fringe Patterns through Development of Weighted Least-Squares Algorithm
Authors: Chen Zhenkai , Zhou Wenjing , Yu Yingjie , Tornari Vivi , Artioli Gilberto
Published: December 2023

In this paper, based on Gaussian 1σ-criterion and histogram segmentation, a weighted least-squares algorithm is applied and validated on digital holographic speckle pattern interferometric data to perform phase separation on the complex interference fields. The direct structural diagnosis tool is used to investigate defects and their impact on a complex antique wall painting of Giotto. The interferometry data is acquired with a portable off-axis interferometer set-up with a phase-shifted reference beam coupled with the object beam in front of the digital photosensitive medium. A digital holographic speckle pattern interferometry (DHSPI) system is used to register digital recordings of interferogram sequences over time. The surface is monitored for as long as it deforms prior to returning to its initial reference equilibrium state prior to excitation. The attempt to separate the whole vs. local defect complex amplitudes from the interferometric data is presented. The main aim is to achieve isolation and visualization of each defect’s impact amplitude in order to obtain detailed documentation of each defect and its structural impact on the surface for structural diagnosis purposes.
Esophageal Cancer with Early Onset in a Patient with Cri du Chat Syndrome
Authors: Danesino Cesare , Gualtierotti Monica , Origi Matteo , Cistaro Angelina , Malacarne Michela , Massidda Matteo , Bencardino Katia , Coviello Domenico , Albani Giovanni , Schiera Irene Giovanna , Liava Alexandra , Guala Andrea
Published: December 2023

Background: In Cri du Chat (CdC), cancer as comorbidity is extremely rare. In databases from Denmark, Spain, Australia, New Zealand, and Japan, no cancer was reported; in Italy and Germany, four cancers were identified out of 321 CdCs. Methods: In a 29-year-old CdC patient, clinical investigations following hematemesis led to the diagnosis of esophageal adenocarcinoma (EAC). A high pain threshold was also observed. Conventional and molecular cytogenetic defined the size of the deletion, and exome analysis on the trio completed the molecular work. Results: Cytogenetic analysis showed a de novo chromosomal alteration: 46,XY,ishdel(5)(p14.3)(D5S28-) and arr[GRCh37] 5p15.33p14.3(1498180_19955760)x1. A quantitative sensory test demonstrated a high heat threshold. A 18f-fluorodeoxyglucose PET/TC scan of the brain failed to detect reduction of metabolism in the somatosensory area or insular cortex. Exome analysis in the trio (patient and parents) failed to identify variants to be interpreted as a likely risk factor for EAC. Conclusion: We conclude that the presence of well-known risk factors (maleness, obesity, gastroesophageal reflux, and Barrett’s metaplasia) in a patient with very limited capability of expressing discomfort or referring clinical symptoms have been the main risk factors for developing EAC. At present, based on the available data, there is no evidence of any increased risk of developing cancer in CdC patients.
Control of the von Neumann Entropy for an Open Two-Qubit System Using Coherent and Incoherent Drives
Authors: Morzhin Oleg , Pechen Alexander
Published: December 2023

This article is devoted to developing an approach for manipulating the von Neumann entropy S(ρ(t)) of an open two-qubit system with coherent control and incoherent control inducing time-dependent decoherence rates. The following goals are considered: (a) minimizing or maximizing the final entropy S(ρ(T)); (b) steering S(ρ(T)) to a given target value; (c) steering S(ρ(T)) to a target value and satisfying the pointwise state constraint S(ρ(t))≤S¯ for a given S¯; (d) keeping S(ρ(t)) constant at a given time interval. Under the Markovian dynamics determined by a Gorini–Kossakowski–Sudarshan–Lindblad type master equation, which contains coherent and incoherent controls, one- and two-step gradient projection methods and genetic algorithm have been adapted, taking into account the specifics of the objective functionals. The corresponding numerical results are provided and discussed.
Radio Frequency Fingerprint Identification for 5G Mobile Devices Using DCTF and Deep Learning
Authors: Fu Hua , Dong Hao , Yin Jian , Peng Linning
Published: December 2023

The fifth-generation (5G) mobile cellular network is vulnerable to various security threats. Radio frequency fingerprint (RFF) identification is an emerging physical layer authentication technique which can be used to detect spoofing and distributed denial of service attacks. In this paper, the performance of RFF identification is studied for 5G mobile phones. The differential constellation trace figure (DCTF) is extracted from the physical random access channel (PRACH) preamble. When the database of all 64 PRACH preambles is available at the gNodeB (gNB), an index-based DCTF identification scheme is proposed, and the classification accuracy reaches 92.78% with a signal-to-noise ratio of 25 dB. Moreover, due to the randomness in the selection of preamble sequences in the random access procedure, when only a portion of the preamble sequences can be trained, a group-based DCTF identification scheme is proposed. The preamble sequences generated from the same root value are grouped together, and the untrained sequences can be identified based on the trained sequences within the same group. The classification accuracy of the group-based scheme is 89.59%. An experimental system has been set up using six 5G mobile phones of three models. The 5G gNB is implemented on the OpenAirInterface platform.
Sustainability and Strategic Assessment of Water and Energy Integration Systems: Case Studies of the Process Industry in Portugal
Authors: Oliveira Miguel Castro , Matos Henrique A.
Published: December 2023

The most recent sustainability policies of each region of the world conjointly define that economic activities shall follow the principles of natural resource use minimisation, as well as eco-efficiency and circular economy promotion, in addition to the specific objectives defined in each policy. Most recently, a group of researchers has proposed innovative conceptual systems designated Water and Energy Integration Systems (WEIS) for issues related to water and energy use (two prominent categories of natural resources). These are based on engineering projects encompassing a multitude of processes and technologies. In this work, an assessment based on the determination of several sustainability and strategic-aims-related indicators is performed for two WEIS case studies set in the Portuguese process industry (in this case, a ceramic plant). Such an assessment serves as an expansion of previously performed studies on the economic and environmental viability associated with the installation of this type of system with the ultimate goal of proving the effective compliance of water- and energy-use-reduction-related results with sustainability and strategic aims (namely, the ones associated with the most recent policies and aspects associated with the social, economic, and environmental pillars of sustainability). The results for the overall assessment proved that the conceptualised WEIS are robust in terms of eco-efficiency, circular economy potential, and strategic objective achievement potential (with a 6.46% and 4.00% improvement for the aggregated eco-efficiency indicator having been obtained for, respectively, case studies 1 and 2, a null water discharge for both case studies, and a level of 8.58% and 6.69% of recirculated heat over total energy consumption, respectively). The obtained results prove the sustainability promotion effectiveness of the WEIS as conceptual systems. The overall set of indicators defined in this work are part of a methodology that may be used and adapted for further studies considering the innovative WEIS approach, with the specific results obtained in this work presented with the aim of their being used for comparison.
Prediction of Mean Heat Transfer Characteristics of Multiple Impinging Jets with Steady RANS Simulation Using a Coarse Mesh
Authors: Draksler Martin , Tekavčič Matej
Published: December 2023

The capability of the standard SST k-ω turbulence model for the prediction of jet impingement cooling characteristics using a coarse mesh is investigated. The discussion is based on a sensitivity study with five computational grids, differing from each other in topology and resolution. The analysis considers a hexagonal configuration of turbulent jets at the inlet Reynolds number equal to 20,000, with the distance between the nozzle and target plates equal to four nozzle diameters. The results of steady RANS simulations are validated against the time-averaged LES results and data from experiments. The mean heat transfer characteristics of turbulent impinging jets have been successfully reproduced with all tested grids, which indicates that for a rather accurate mean heat transfer prediction, it is not necessary to resolve all the small-scale flow features of impinging jets above the target plate.
Multi-Scale Characterization of Porosity and Cracks in Silicon Carbide Cladding after Transient Reactor Test Facility Irradiation
Authors: Xu Fei , Yao Tiankai , Xu Peng , Schulthess Jason L. , Matos Mario D. , Gonderman Sean , Gazza Jack , Kane Joshua J. , Cordes Nikolaus L.
Published: December 2023

Silicon carbide (SiC) ceramic matrix composite (CMC) cladding is currently being pursued as one of the leading candidates for accident-tolerant fuel (ATF) cladding for light water reactor applications. The morphology of fabrication defects, including the size and shape of voids, is one of the key challenges that impacts cladding performance and guarantees reactor safety. Therefore, quantification of defects’ size, location, distribution, and leak paths is critical to determining SiC CMC in-core performance. This research aims to provide quantitative insight into the defect’s distribution under multi-scale characterization at different length scales before and after different Transient Reactor Test Facility (TREAT) irradiation tests. A non-destructive multi-scale evaluation of irradiated SiC will help to assess critical microstructural defects from production and/or experimental testing to better understand and predict overall cladding performance. X-ray computed tomography (XCT), a non-destructive, data-rich characterization technique, is combined with lower length scale electronic microscopic characterization, which provides microscale morphology and structural characterization. This paper discusses a fully automatic workflow to detect and analyze SiC-SiC defects using image processing techniques on 3D X-ray images. Following the XCT data analysis, advanced characterizations from focused ion beam (FIB) and transmission electron microscopy (TEM) were conducted to verify the findings from the XCT data, especially quantitative results from local nano-scale TEM 3D tomography data, which were utilized to complement the 3D XCT results. In this work, three SiC samples (two irradiated and one unirradiated) provided by General Atomics are investigated. The irradiated samples were irradiated in a way that was expected to induce cracking, and indeed, the automated workflow developed in this work was able to successfully identify and characterize the defects formation in the irradiated samples while detecting no observed cracking in the unirradiated sample. These results demonstrate the value of automated XCT tools to better understand the damage and damage propagation in SiC-SiC structures for nuclear applications.
Design and Analysis of a Hybrid Stand-Alone Microgrid
Authors: Bhatti Muhammad Zubair Asif , Siddique Abubakar , Aslam Waseem , Atiq Shahid
Published: December 2023

This research article presents a comprehensive investigation into the design, optimization, and performance analysis of a hybrid stand-alone microgrid for an industrial facility in Iraq at coordinates 36.51 and 43.99. The system consists of photovoltaic (PV) modules, inverters, a battery energy storage system (BESS), a generator, and AC loads. Leveraging the capabilities of PVsyst version 7.3.1, HOMER Pro version 3.14.2 and SAM version 2022.11.21 software tools, this study assesses the feasibility and functionality of the hybrid stand-alone microgrid. In this study, PVsyst software is used for detailed designing and analysis of a PV plant, and the PVsyst design file is then used in HOMER Pro software to optimize and design the proposed hybrid stand-alone microgrid, and for detailed performance analysis SAM software is employed. This paper also investigates the impact of ground clearance and ground albedo on the annual generation of bifacial PV modules at various tilt angles. Key findings include a promising normalized production rate of 4.53 kWh/kWp/day with a performance ratio of 0.815 and annual energy production estimates of 84.31 MWh (P50), 79.57 MWh (P90), and 78.24 MWh (P95) for monofacial PV modules, highlighting the system’s potential for renewable energy generation. Notably, this research demonstrates the hybrid stand-alone microgrid’s capacity to significantly reduce CO2 emissions, saving approximately 1811.6 tons over a 30-year period, thus contributing to sustainability and environmental conservation goals. Additionally, this study reveals operational challenges during the winter months, necessitating generator support to meet load demands. The successful installation and experimental validation of the hybrid stand-alone microgrid underscore its practical viability and its role in advancing clean energy solutions. This research provides valuable insights into hybrid stand-alone microgrid design, emphasizing its importance in ensuring reliable power supply and environmental stewardship.
Coconut Juice Enhances Rooting and Leaf Essential Oils of Juniperus sabina L. Cuttings
Authors: Abshahi Maliheh , García-Morote Francisco Antonio , Zarei Hossein , Zahedi Bahman , Nejad Abdolhossein Rezaei
Published: December 2023

Juniperus sabina L. (J. sabina L.) represents a compelling forest species due to the presence of metabolites in its leaves, which possess diverse applications in the fields of cosmetics, pharmaceuticals, and medicine. However, the species presents difficulties with regard to natural regeneration. This research aimed to improve the propagation conditions of J. sabina by using cuttings to enhance both the rooting percentage and essential oil (EO) content in the leaves. To do this, sampled cuttings underwent pretreatment with four different doses of coconut juice (25%, 50%, 75%, and 100%) within four substrates or rooting media, namely perlite, mixed substrate, pumice, and perlite–cocopeat. The study was carried out over the course of all four growing seasons to examine the impact of harvesting time as well. The best results for rooting were observed with the pretreatment of coconut juice at 25% in spring and in the substrate of perlite–cocopeat (37.10% rooting). The coconut juice also significantly increased the percentage of EOs in spring (0.82% of essential oil yield). Beta-pinene was the essential oil component with the highest representation (34.7% in fall), whereas in the spring, the best season to collect cuttings, the dominant component was nerodiol. Our study can contribute to the planning of culture systems using cuttings and to the control of the production of EOs from the leaves of J. sabina.
Physiological, Photosynthetic and Stomatal Ultrastructural Responses of Quercus acutissima Seedlings to Drought Stress and Rewatering
Authors: Liu Dan , Guo Haili , Yan Li-Ping , Gao Lei , Zhai Shasha , Xu Yan
Published: December 2023

The physiological mechanisms of drought-stress response in Quercus acutissima were explored with the aim to develop potentially valuable drought-resistant species that are adapted to arid regions and barren mountains. Potting experiments of Q. acutissima that simulated drought-stress conditions, and morphological, physiological, photosynthetic, and ultrastructural changes were investigated at different stages of drought stress, including after rehydration and recovery. During drought stress and rewatering, the leaves exhibited yellowing and abscission, followed by the sprouting of new leaf buds. The relative water content (RWC) changed under the drought-rewatering treatment, with a decreasing and then increasing trend, while the relative electrical conductivity (REC) had a more gradual increasing and then decreasing trend. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and the proline (Pro) and soluble sugar (SS) contents increased and then decreased. The change in soluble protein (SP) content showed an N-shaped trend of increasing, decreasing, and then increasing again. The malondialdehyde (MDA) content decreased and then slowly increased. From the drought to recovery phase of the experiment, the net photosynthesis (Pn), stomatal conductance (Gs), and transpiration rate (Tr) decreased gradually at first, and then the Pn increased significantly, while the Gs and Tr increased slowly. During this period, the internal CO2 concentration (Ci) did not decrease significantly until the last stage of the drought treatment, and then it increased slowly thereafter. The open stomata count in the Q. acutissima leaves was reduced significantly as drought stress increased, but after rewatering, the stomata recovered rapidly, with their opening size increasing. The number of leaf epidermal trichomes gradually declined to a low count in response to drought stress, but it rapidly recovered and increased within a short period of time after rehydration. Q. acutissima was found to have a strong drought tolerance and recovery ability after exposure to drought stress, and it may be an effective pioneer species for reforestation in barren lands.
Quantification of Expected Return of Investment in Wood Processing Sectors in Slovakia
Authors: Kánová Martina , Drábek Josef , Ćurić Petar , Pirc Barčić Andreja
Published: December 2023

The study focuses on the selected aspects of investment measurement and management for the support of financial and economic decision-making of investors in wood-processing sectors. The aim of the study was to analyze the indicators for the structure and cost of capital of furniture and paper/forest branches in Slovakia, quantify the actual expected return on investment based on the selected methodology, and consequently find out the fundamental differences resulting from the specific conditions of given sectors. Methodologically, the study uses procedures for the weighted average cost of capital (WACC), capital asset pricing model (CAPM) for determining the cost of equity, and calculation of the beta coefficient considering the risk premium. The results of the study demonstrated a similar levered beta in both analyzed sectors (1.17 in furniture, 1.20 in paper/forest), but in each sector for a different reason. The expected rate of return is higher in furniture (7.84%) compared to paper/forest products at the level of 6.04%. The findings provide the possibility of comparing the required and expected rate of return on invested capital and making the appropriate long-term investment decisions.
Transcriptional Analysis of Antrodin C Synthesis in Taiwanofungus camphoratus (Syn. Antrodia camphorate, Antrodia cinnamomea) to Understand Its Biosynthetic Mechanism
Authors: Jia Wei , Gai Shu-Ping , Li Xiao-Hui , Zhang Jing-Song , Wang Wen-Han
Published: December 2023

Antrodin C, a bioactive component of Taiwanofungus camphoratus, exhibits good immunophysiological and antitumour activities, including a broad spectrum of anticancer effects. Exogenous additives can bind to metabolites during the submerged culture of T. camphoratus and affect secondary metabolite yields. However, the lack of molecular genetic studies on T. camphoratus has hindered the study of the antrodin C biosynthetic pathway. In this study, we conducted a ribonucleic acid-sequencing-based transcriptional analysis to identify the differentially expressed genes involved in the synthesis of antrodin C by T. camphoratus, using inositol and maleic acid (MAC) as exogenous additives. The addition of inositol significantly upregulated carbohydrate and sugar metabolism pathway genes (E3.2.1.14, UGDH, and IVD). When MAC was used, amino and nucleotide sugar metabolism and starch and sucrose metabolism pathways were significantly inhibited, and the associated genes (E3.2.1.14 and E3.2.1.58) were also significantly downregulated. The biosynthesis pathway genes for ubiquinone and other terpene quinones (COQ2, ARO8, and wrbA), which may play an important role in antrodin C synthesis, were significantly downregulated. This study advances our understanding of how the additives inositol and MAC affect metabolite biosynthesis in T. camphorates. This could be beneficial in proposing potential strategies for improving antrodin C production using a genetic approach.
Adaptation of the European Fish Index (EFI+) to Include the Alien Fish Pressure
Authors: Aparicio Enric , Alcaraz Carles , Rocaspana Rafel , Pou-Rovira Quim , García-Berthou Emili
Published: December 2023

The European Fish Index EFI+ is the only fish-based multimetric index for the assessment of the ecological status of running waters that is validated and thus applicable across most countries of the European Union. Metrics of the index rely on several attributes of the species present in the fish assemblage, irrespective of their native/alien status. The abundance of alien fish, together with other anthropogenic impacts, is one of the most important threats to the conservation of native fish and ecosystem health and is also an indicator of degraded stream conditions. Therefore, to improve the performance of the EFI+ in regions with high incidence of alien species, the EFI+ was adapted to include alien fish pressure as a new metric that reflects the number of alien species as well as the proportional abundance of alien individuals. The application of the adapted index (A-EFI+) is illustrated with data from several Iberian Mediterranean basins and showed similar or stronger correlations than the original EFI+ with anthropogenic pressure (land-use variables and alterations in hydrology and river morphology) and with other regional fish indices. EFI+ has been invaluable to intercalibrate fish indices across Europe, and A-EFI+ is similar but explicitly includes alien pressure, thus helping to provide a more comprehensive assessment of ecosystem health and to communicate it to society.
Novel Fuzzy Contractions and Applications to Engineering Science
Authors: Younis Mudasir , Abdou Afrah Ahmad Noman
Published: December 2023

The article outlines a creative approach by fusing ideas from fuzzy contractions, graph mappings, and Kannan mappings to create a brand-new notion known as “Kannan-graph-fuzzy contraction”. This combination of concepts makes it possible to create a unique framework for resolving real-world issues controlled by nonlinear models. It is anticipated that using Kannan-graph-fuzzy contractions will offer a fresh approach to discussing solution existence in situations where these ideas overlap. This strategy might have a significant impact on a lot of real-world applications, proving how practical multidisciplinary techniques are for tackling issues in the real world originating from technology and engineering. The article includes several illustrated examples based on computer simulations, making the results more explicit.
Exploring the Regulation and Function of Rpl3l in the Development of Early-Onset Dilated Cardiomyopathy and Congestive Heart Failure Using Systems Genetics Approach
Authors: Bajpai Akhilesh K. , Gu Qingqing , Orgil Buyan-Ochir , Alberson Neely R. , Towbin Jeffrey A. , Martinez Hugo R. , Lu Lu , Purevjav Enkhsaikhan
Published: December 2023

Background: Cardiomyopathies, diseases affecting the myocardium, are common causes of congestive heart failure (CHF) and sudden cardiac death. Recently, biallelic variants in ribosomal protein L3-like (RPL3L) have been reported to be associated with severe neonatal dilated cardiomyopathy (DCM) and CHF. This study employs a systems genetics approach to gain understanding of the regulatory mechanisms underlying the role of RPL3L in DCM. Methods: Genetic correlation, expression quantitative trait loci (eQTL) mapping, differential expression analysis and comparative functional analysis were performed using cardiac gene expression data from the patients and murine genetic reference populations (GRPs) of BXD mice (recombinant inbred strains from a cross of C57BL/6J and DBA/2J mice). Additionally, immune infiltration analysis was performed to understand the relationship between DCM, immune cells and RPL3L expression. Results: Systems genetics analysis identified high expression of Rpl3l mRNA, which ranged from 11.31 to 12.16 across murine GRPs of BXD mice, with an ~1.8-fold difference. Pathways such as “diabetic cardiomyopathy”, “focal adhesion”, “oxidative phosphorylation” and “DCM” were significantly associated with Rpl3l. eQTL mapping suggested Myl4 (Chr 11) and Sdha (Chr 13) as the upstream regulators of Rpl3l. The mRNA expression of Rpl3l, Myl4 and Sdha was significantly correlated with multiple echocardiography traits in BXD mice. Immune infiltration analysis revealed a significant association of RPL3L and SDHA with seven immune cells (CD4, CD8-naive T cell, CD8 T cell, macrophages, cytotoxic T cell, gamma delta T cell and exhausted T cell) that were also differentially infiltrated between heart samples obtained from DCM patients and normal individuals. Conclusions: RPL3L is highly expressed in the heart tissue of humans and mice. Expression of Rpl3l and its upstream regulators, Myl4 and Sdha, correlate with multiple cardiac function traits in murine GRPs of BXD mice, while RPL3L and SDHA correlate with immune cell infiltration in DCM patient hearts, suggesting important roles for RPL3L in DCM and CHF pathogenesis via immune inflammation, necessitating experimental validations of Myl4 and Sdha in Rpl3l regulation.
Medical Gaslighting and Lyme Disease: The Patient Experience
Authors: Fagen Jennifer L. , Shelton Jeremy A. , Luché-Thayer Jenna
Published: December 2023

Even though there are approximately half a million new cases of Lyme disease in the US annually, according to the CDC, it is often undiagnosed or misdiagnosed, which can result in a chronic, multisystemic condition. Lyme disease is a recognized public health threat and is a designated “notifiable disease”. As such, Lyme disease is mandated to be reported by the CDC. Despite this, both acute and chronic Lyme disease (CLD) have been relegated to the category of “contested illnesses”, which can lead to medical gaslighting. By analyzing results from an online survey of respondents with Lyme disease (n = 986), we elucidate the lived experiences of people who have been pushed to the margins of the medical system by having their symptoms attributed to mental illness, anxiety, stress, and aging. Further, respondents have had their blood tests and erythema migrans (EM) rashes discounted and were told that CLD simply does not exist. As a result, a series of fruitless consultations often result in the delay of a correct diagnosis, which has deleterious consequences. This is the first study that addresses an extensive range of gaslighting techniques experienced by this patient population.
Microbes and Mental Illness: Past, Present, and Future
Authors: Bransfield Robert C. , Mao Charlotte , Greenberg Rosalie
Published: December 2023

A review of the association between microbes and mental illness is performed, including the history, relevant definitions, infectious agents associated with mental illnesses, complex interactive infections, total load theory, pathophysiology, psychoimmunology, psychoneuroimmunology, clinical presentations, early-life infections, clinical assessment, and treatment. Perspectives on the etiology of mental illness have evolved from demonic possession toward multisystem biologically based models that include gene expression, environmental triggers, immune mediators, and infectious diseases. Microbes are associated with a number of mental disorders, including autism, schizophrenia, bipolar disorder, depressive disorders, and anxiety disorders, as well as suicidality and aggressive or violent behaviors. Specific microbes that have been associated or potentially associated with at least one of these conditions include Aspergillus, Babesia, Bartonella, Borna disease virus, Borrelia burgdorferi (Lyme disease), Candida, Chlamydia, coronaviruses (e.g., SARS-CoV-2), Cryptococcus neoformans, cytomegalovirus, enteroviruses, Epstein–Barr virus, hepatitis C, herpes simplex virus, human endogenous retroviruses, human immunodeficiency virus, human herpesvirus-6 (HHV-6), human T-cell lymphotropic virus type 1, influenza viruses, measles virus, Mycoplasma, Plasmodium, rubella virus, Group A Streptococcus (PANDAS), Taenia solium, Toxoplasma gondii, Treponema pallidum (syphilis), Trypanosoma, and West Nile virus. Recognition of the microbe and mental illness association with the development of greater interdisciplinary research, education, and treatment options may prevent and reduce mental illness morbidity, disability, and mortality.
Development and Validation of a Questionnaire for Assessing Drug Use Motives in the General Population in South Korea
Authors: Yang Joon-Yong , Kim Minhye , Sohn Aeree
Published: December 2023

Drug use is increasing in South Korea, necessitating evidence-based policy interventions. However, there is a dearth of reliable tools for identifying the personal and psychological factors that drive drug use among Korean adults. In this study, we developed and validated an instrument based on a motivational model to measure drug use motivation in the Korean context. A survey was administered online to 250 Korean adults aged 19–59. Of the initial 37 potential items evaluated, 23 were retained after exploratory factor analysis. Based on their relevance and factor loadings, the final scale consisted of 15 items distributed across five distinct factors: enhancement, coping, social, positive expectancy, and negative expectancy. Confirmatory factor analysis validated the strong fit of the five-factor model. Criterion-related validity was demonstrated by the significant correlations between the five factors and the selected criterion variables. This instrument can be used in research initiatives related to drug addiction and can provide basic data for policy interventions intended to curb drug addiction problems in South Korea. The novelty and broad applicability of this instrument make it invaluable for exploring the psychological underpinnings of drug use in South Korea.
Response to Deficit Irrigation of ‘Orogrande’ Mandarin Grafted onto Different Citrus Rootstocks in Spain
Authors: Primo-Capella Amparo , Molina-Nadal María Dolores , Catalá-Senent Laura , de Miguel-Moreno Agustí , Forner-Giner María Ángeles , Martínez-Cuenca Mary-Rus
Published: December 2023

Drought is increasingly becoming an abiotic stress factor that affects citrus yield in the Mediterranean Basin, and rootstocks may impact the plants’ responses to it. This study compares the influences of Forner-Alcaide 5 (FA-5), an emerging well-established rootstock in Spain, and Carrizo citrange (CC), the common commercial rootstock of the Orogrande mandarin variety, on plants’ responses to water reduction. The deficit irrigation (DI) condition was established by 50% irrigation and evapotranspiration (ETc). The canopy volume, yield, fruit size and fruit internal quality were evaluated. The yield reduction in the CC DI was mainly due to a smaller fruit size, but in FA-5, it was due to fewer fruits without an affected caliber. Regarding Ct, the fruits from the CC DI had lower juice contents and higher rind percentages, while the differences between the Ct and DI trees were smaller in the FA-5 rootstock. The most remarkable effect was the increase in total soluble sugars (TSS) for the DI treatment. To conclude, the FA-5 rootstock had the strongest influence on Orogrande mandarin under water stress. These results can be useful for addressing water stress problems in citrus.
The Mediating Role of Procrastination in the Relationship between Fear of Missing Out and Internet Addiction in University Students
Authors: Manap Abdullah , Rizzo Amelia , Yıldırmaz Abdullah , Dilekçi Ümit , Yıldırım Murat
Published: December 2023

This study aims to examine the potential mediating role of procrastination in the relationship between fear of missing out and internet addiction. Employing a cross-sectional research design, this study utilized a paper–pencil form to collect data from 315 students (65.4% undergraduate and 66.3% females) between 18 and 32 (Mage = 22.43; SD = 3.81) studying at a state university in Türkiye. Data were collected through the convenience sampling method. The study was designed as a survey model. This design was tested via mediation analysis. The participants completed self-report assessments using the Fear of Missing Out Scale (FoMOs), General Procrastination Scale (GPS-9), and Young Internet Addiction Scale (short form). The findings showed that FoMO had a significant positive direct effect on both procrastination (R2 = 0.13) and internet addiction (R2 = 0.33). Procrastination also had a significant positive direct effect on internet addiction (R2 = 0.34). Additionally, procrastination mediated the relationship between the fear of missing out and internet addiction (β = 0.156, p < 0.001). These findings not only extend the scope of existing research but also hold practical implications for the development of sustainable interventions. It is believed that the findings will contribute to the consideration of procrastination when preparing psychoeducation or group guidance programs for internet addiction. These interventions can effectively address the process through which fear of missing out leads to internet addiction by considering the significant role of procrastination in students. Findings typically suggest that procrastination behavior is a key factor in explaining the association between FoMO and internet addiction.
Pregnancy Desirability and Motivational Readiness for Postpartum Contraceptive Use: Findings from Population-Based Surveys in Eight Sub-Saharan African Countries
Authors: Ujah Otobo I. , Olagbuji Biodun N. , Ogbu Chukwuemeka E. , Ujah Innocent A. O. , Kirby Russell S.
Published: December 2023

This study examined the associations between pregnancy intention and motivational readiness for postpartum contraceptive use. Data for this cross-sectional analysis were derived from nationally representative surveys of the Performance Monitoring and Accountability 2020 (PMA2020) project conducted in eight sub-Saharan African countries. Participants included 9488 nonpregnant women of reproductive age (15–49 years) who had given birth in the last 2 years. Weighted multinomial logistic regression analyses were performed to estimate the odds ratios (OR) and their corresponding 95% confidence intervals (CIs) of the associations of motivational readiness for contraceptive adoption categorized as precontemplation, contemplation, and post-action with pregnancy intention. After adjusting for confounding factors, the findings revealed that women in Côte d’Ivoire and Nigeria who had mistimed pregnancies had significantly higher odds of being in the contemplation vs. precontemplation stage compared to those who had intended pregnancies. Similarly, women who had unwanted pregnancies in Ethiopia were also more likely to be in the contemplation stage. Furthermore, significant differences were observed for women in Burkina Faso, Côte d’Ivoire, and Nigeria regarding the association between mistimed pregnancies and being in the post-action stage. For women who had unwanted pregnancies, this association was significant only in Nigeria. Additionally, the odds of being in the contemplation stage, compared to the post-action stage, for women who had unwanted pregnancies were significantly higher in Ethiopia and Nigeria. These results indicate that recent unintended pregnancies in specific sub-Saharan African countries may motivate women to take action to prevent future unintended pregnancies. The findings underscore the importance of tailored and context-specific approaches in family-planning programs based on the stage of motivational readiness.
Target Prediction by Multiple Virtual Screenings: Analyzing the SARS-CoV-2 Phenotypic Screening by the Docking Simulations Submitted to the MEDIATE Initiative
Authors: Gervasoni Silvia , Manelfi Candida , Adobati Sara , Talarico Carmine , Biswas Akash Deep , Pedretti Alessandro , Vistoli Giulio , Beccari Andrea R.
Published: December 2023

Phenotypic screenings are usually combined with deconvolution techniques to characterize the mechanism of action for the retrieved hits. These studies can be supported by various computational analyses, although docking simulations are rarely employed. The present study aims to assess if multiple docking calculations can prove successful in target prediction. In detail, the docking simulations submitted to the MEDIATE initiative are utilized to predict the viral targets involved in the hits retrieved by a recently published cytopathic screening. Multiple docking results are combined by the EFO approach to develop target-specific consensus models. The combination of multiple docking simulations enhances the performances of the developed consensus models (average increases in EF1% value of 40% and 25% when combining three and two docking runs, respectively). These models are able to propose reliable targets for about half of the retrieved hits (31 out of 59). Thus, the study emphasizes that docking simulations might be effective in target identification and provide a convincing validation for the collaborative strategies that inspire the MEDIATE initiative. Disappointingly, cross-target and cross-program correlations suggest that common scoring functions are not specific enough for the simulated target.
Tamoxifen Activates Transcription Factor EB and Triggers Protective Autophagy in Breast Cancer Cells by Inducing Lysosomal Calcium Release: A Gateway to the Onset of Endocrine Resistance
Authors: Boretto Cecilia , Actis Chiara , Faris Pawan , Cordero Francesca , Beccuti Marco , Ferrero Giulio , Muzio Giuliana , Moccia Francesco , Autelli Riccardo
Published: December 2023

Among the several mechanisms accounting for endocrine resistance in breast cancer, autophagy has emerged as an important player. Previous reports have evidenced that tamoxifen (Tam) induces autophagy and activates transcription factor EB (TFEB), which regulates the expression of genes controlling autophagy and lysosomal biogenesis. However, the mechanisms by which this occurs have not been elucidated as yet. This investigation aims at dissecting how TFEB is activated and contributes to Tam resistance in luminal A breast cancer cells. TFEB was overexpressed and prominently nuclear in Tam-resistant MCF7 cells (MCF7-TamR) compared with their parental counterpart, and this was not dependent on alterations of its nucleo-cytoplasmic shuttling. Tam promoted the release of lysosomal Ca2+ through the major transient receptor potential cation channel mucolipin subfamily member 1 (TRPML1) and two-pore channels (TPCs), which caused the nuclear translocation and activation of TFEB. Consistently, inhibiting lysosomal calcium release restored the susceptibility of MCF7-TamR cells to Tam. Our findings demonstrate that Tam drives the nuclear relocation and transcriptional activation of TFEB by triggering the release of Ca2+ from the acidic compartment, and they suggest that lysosomal Ca2+ channels may represent new druggable targets to counteract the onset of autophagy-mediated endocrine resistance in luminal A breast cancer cells.
The Collaborative Cross-Mouse Population for Studying Genetic Determinants Underlying Alveolar Bone Loss Due to Polymicrobial Synergy and Dysbiosis
Authors: Nashef Aysar , Qabaja Rawan , Hazan Ronen , Schafer Arne , Hasturk Hatice , Kantarci Alpdogan , Houri-Haddad Yael , Iraqi Fuad A.
Published: December 2023

Dysbiosis of oral microbiota is associated with the initiation and progression of periodontitis. The cause-and-effect relationship between genetics, periodontitis, and oral microbiome dysbiosis is poorly understood. Here, we demonstrate the power of the collaborative cross (CC) mice model to assess the effect of the genetic background on microbiome diversity shifts during periodontal infection and host suitability status. We examined the bacterial composition in plaque samples from seven different CC lines using 16s rRNA sequencing before and during periodontal infection. The susceptibility/resistance of the CC lines to alveolar bone loss was determined using the micro-CT technique. A total of 53 samples (7 lines) were collected before and after oral infection using oral swaps followed by DNA extraction and 16 s rRNA sequencing analysis. CC lines showed a significant variation in response to the co-infection (p < 0.05). Microbiome compositions were significantly different before and after infection and between resistant and susceptible lines to periodontitis (p < 0.05). Gram-positive taxa were significantly higher at the resistant lines compared to susceptible lines (p < 0.05). Gram-positive bacteria were reduced after infection, and gram-negative bacteria, specifically anaerobic groups, increased after infection. Our results demonstrate the utility of the CC mice in exploring the interrelationship between genetic background, microbiome composition, and periodontitis.
Effects of Mycobacterium vaccae NCTC 11659 and Lipopolysaccharide Challenge on Polarization of Murine BV-2 Microglial Cells
Authors: Desmond Luke W. , Holbrook Evan M. , Wright Caelan T. O. , Zambrano Cristian A. , Stamper Christopher E. , Bohr Adam D. , Frank Matthew G. , Podell Brendan K. , Moreno Julie A. , MacDonald Andrew S. , Reber Stefan O. , Hernández-Pando Rogelio , Lowry Christopher A.
Published: December 2023

Previous studies have shown that the in vivo administration of soil-derived bacteria with anti-inflammatory and immunoregulatory properties, such as Mycobacterium vaccae NCTC 11659, can prevent a stress-induced shift toward an inflammatory M1 microglial immunophenotype and microglial priming in the central nervous system (CNS). It remains unclear whether M. vaccae NCTC 11659 can act directly on microglia to mediate these effects. This study was designed to determine the effects of M. vaccae NCTC 11659 on the polarization of naïve BV-2 cells, a murine microglial cell line, and BV-2 cells subsequently challenged with lipopolysaccharide (LPS). Briefly, murine BV-2 cells were exposed to 100 µg/mL whole-cell, heat-killed M. vaccae NCTC 11659 or sterile borate-buffered saline (BBS) vehicle, followed, 24 h later, by exposure to 0.250 µg/mL LPS (Escherichia coli 0111: B4; n = 3) in cell culture media vehicle (CMV) or a CMV control condition. Twenty-four hours after the LPS or CMV challenge, cells were harvested to isolate total RNA. An analysis using the NanoString platform revealed that, by itself, M. vaccae NCTC 11659 had an “adjuvant-like” effect, while exposure to LPS increased the expression of mRNAs encoding proinflammatory cytokines, chemokine ligands, the C3 component of complement, and components of inflammasome signaling such as Nlrp3. Among LPS-challenged cells, M. vaccae NCTC 11659 had limited effects on differential gene expression using a threshold of 1.5-fold change. A subset of genes was assessed using real-time reverse transcription polymerase chain reaction (real-time RT-PCR), including Arg1, Ccl2, Il1b, Il6, Nlrp3, and Tnf. Based on the analysis using real-time RT-PCR, M. vaccae NCTC 11659 by itself again induced “adjuvant-like” effects, increasing the expression of Il1b, Il6, and Tnf while decreasing the expression of Arg1. LPS by itself increased the expression of Ccl2, Il1b, Il6, Nlrp3, and Tnf while decreasing the expression of Arg1. Among LPS-challenged cells, M. vaccae NCTC 11659 enhanced LPS-induced increases in the expression of Nlrp3 and Tnf, consistent with microglial priming. In contrast, among LPS-challenged cells, although M. vaccae NCTC 11659 did not fully prevent the effects of LPS relative to vehicle-treated control conditions, it increased Arg1 mRNA expression, suggesting that M. vaccae NCTC 11659 induces an atypical microglial phenotype. Thus, M. vaccae NCTC 11659 acutely (within 48 h) induced immune-activating and microglial-priming effects when applied directly to murine BV-2 microglial cells, in contrast to its long-term anti-inflammatory and immunoregulatory effects observed on the CNS when whole-cell, heat-killed preparations of M. vaccae NCTC 11659 were given peripherally in vivo.