bluuebunny/crossref_metadata_2025 huggingface dataset:
Suitability Evaluation of Urban Construction Land Based on an Approach of Vertical-Horizontal Processes
Authors: Yan Yuetian , Zhou Rui , Ye Xinyue , Zhang Hao , Wang Xinjun
Published: May 2018

Suitability evaluation of urban construction land is critical for both urban master planning and the proper utilization of land resources. Using the Beihu New District of Jining City, China, as a case study, this paper introduces a novel research approach for comprehensive suitability evaluation based on vertical-horizontal processes. First, by considering both the land development potential and ecological constraint resistance, the potential-resistance (PR) model was developed and used to analyze the suitability for urban construction of vertical processes. Then, given the results of the vertical suitability analysis, the current urban built-up areas were selected as the sources of urban expansion, and the minimum cumulative resistance (MCR) model was applied to evaluate the suitability for urban development in terms of horizontal processes. The study area was regionalized into four categories—priority, suitable, restricted, and prohibited areas—which were defined based on the development threshold. The results showed that restricted and prohibited areas for urban construction occupied most of the study area. Totally, 648.51 km2 was categorized as restricted or prohibited, accounting for 12.89% and 54.75% of the total area, respectively. Priority and suitable areas for urban construction covered a total area of 310.37 km2, accounting for 16.55% and 15.81% of the total area, respectively. These areas were mainly distributed around urban centers and urban built-up areas. These findings reflect the substantial potential for future urban development and construction in the study area. The newly developed principles and methods of suitability evaluation for urban construction land presented in this paper provide more appropriate scales and spatial location for urban development and an ecological baseline for future urban growth.
Effect of Temperature Changes on Serum Protein Adsorption on Thermoresponsive Cell-Culture Surfaces Monitored by A Quartz Crystal Microbalance with Dissipation
Authors: Kobayashi Jun , Arisaka Yoshinori , Yui Nobuhiko , Akiyama Yoshikatsu , Yamato Masayuki , Okano Teruo
Published: May 2018

Thermoresponsive cell-culture polystyrene (PS) surfaces that are grafted with poly(N-isopropylacrylamide) (PIPAAm) facilitate the cultivation of cells at 37 °C and the detachment of cultured cells as a sheet with an underlying extracellular matrix (ECM) by reducing the temperature. However, the ECM and cell detachment mechanisms are still unclear because the detachment of cells from thermoresponsive surfaces is governed by complex interactions among the cells/ECM/surface. To explore the dynamic behavior of serum protein adsorption/desorption, thermoresponsive surfaces that correspond to thermoresponsive tissue-culture PS dishes were formed on sensor chips for quartz crystal microbalance with dissipation (QCM-D) measurements. X-ray photoelectron spectroscopy (XPS) measurements and temperature-dependent frequency and dissipation shifts, Δf and ΔD, using QCM-D revealed that the thermoresponsive polymers were successfully grafted onto oxidized, thin PS films on the surfaces of the sensor chips. Increased amounts of adsorbed bovine serum albumin (BSA) and fibronectin (FN) were observed on the thermoresponsive polymer-grafted surfaces at 37 °C when compared with those at 20 °C because of enhanced hydrophobic interactions with the hydrophobic, thermoresponsive surface. While the calculated masses of adsorbed BSA and FN using QCM-D were 3–5 times more than those that were obtained from radiolabeling, the values were utilized for relative comparisons among the same substrate. More importantly, the thermoresponsive, dynamic behavior of serum protein adsorption/desorption was monitored using the QCM-D technique. Observations of this dynamic behavior revealed that the BSA and FN that were adsorbed at 37 °C remained on both surfaces after decreasing the temperature to 20 °C.
Hyaluronic Acid Influence on Normal and Osteoarthritic Tissue-Engineered Cartilage
Authors: Hemmati-Sadeghi Shabnam , Ringe Jochen , Dehne Tilo , Haag Rainer , Sittinger Michael
Published: May 2018

The aim of this study is to identify gene expression profiles associated with hyaluronic acid (HA) treatment of normal and osteoarthritis (OA)-like tissue-engineered cartilage. 3D cartilage micromasses were treated with tumour necrosis factor-α (TNF-α) (OA-inducer) and/or HA for 7 days. Viability was examined by PI/FDA staining. To document extracellular matrix (ECM) formation, glycosaminoglycans (GAG) were stained with Safranin-O and cartilage-specific type II collagen was detected immunohistochemically. Genome-wide gene expression was determined using microarray analysis. Normal and OA-like micromasses remained vital and showed a spherical morphology and homogenous cell distribution regardless of the treatment. There was no distinct difference in immunolabeling for type II collagen. Safranin-O staining demonstrated a typical depletion of GAG in TNF-α-treated micromasses (−73%), although the extent was limited in the presence of HA (−39%). The microarray data showed that HA can influence the cartilage metabolism via upregulation of TIMP3 in OA-like condition. The upregulation of VEGFA and ANKRD37 genes implies a supportive role of HA in cartilage maturation and survival. The results of this study validate the feasibility of the in vitro OA model for the investigation of HA. On the cellular level, no inhibiting or activating effect of HA was shown. Microarray data demonstrated a minor impact of HA on gene expression level.
Cell Propagation of Cholera Toxin CTA ADP-Ribosylating Factor by Exosome Mediated Transfer
Authors: Zanetti Cristiana , Gallina Angelo , Fabbri Alessia , Parisi Sofia , Palermo Angela , Fecchi Katia , Boussadia Zaira , Carollo Maria , Falchi Mario , Pasquini Luca , Fiani Maria Luisa , Sargiacomo Massimo
Published: May 2018

In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population. The first evidence for long-term transmission of CT’s toxic effect via extracellular vesicles was obtained in Chinese hamster ovary (CHO) cells. To follow the CT intracellular route towards exosome secretion, we used a novel strategy for generating metabolically-labeled fluorescent exosomes that can be counted by flow cytometry assay (FACS) and characterized. Our results clearly show the association of CT with exosomes, together with the heat shock protein 90 (HSP90) and Protein Disulfide Isomerase (PDI) molecules, proteins required for translocation of CTA across the ER membrane into the cytoplasm. Confocal microscopy showed direct internalization of CT containing fluorescent exo into CHO cells coupled with morphological changes in the recipient cells that are characteristic of CT action. Moreover, Me665 cells treated with CT-containing exosomes showed an increase in Adenosine 3’,5’-Cyclic Monophosphate (cAMP) level, reaching levels comparable to those seen in cells exposed directly to CT. Our results prompt the idea that CT can exploit an exosome-mediated cell communication pathway to extend its pathophysiological action beyond an initial host cell, into a multitude of cells. This finding could have implications for cholera disease pathogenesis and epidemiology.
Hyperoxia Disrupts Extracellular Signal-Regulated Kinases 1/2-Induced Angiogenesis in the Developing Lungs
Authors: Menon Renuka T. , Shrestha Amrit Kumar , Barrios Roberto , Shivanna Binoy
Published: May 2018

Hyperoxia contributes to the pathogenesis of bronchopulmonary dysplasia (BPD), a chronic lung disease of infants that is characterized by interrupted alveologenesis. Disrupted angiogenesis inhibits alveologenesis, but the mechanisms of disrupted angiogenesis in the developing lungs are poorly understood. In pre-clinical BPD models, hyperoxia increases the expression of extracellular signal-regulated kinases (ERK) 1/2; however, its effects on the lung endothelial ERK1/2 signaling are unclear. Further, whether ERK1/2 activation promotes lung angiogenesis in infants is unknown. Hence, we tested the following hypotheses: (1) hyperoxia exposure will increase lung endothelial ERK1/2 signaling in neonatal C57BL/6J (WT) mice and in fetal human pulmonary artery endothelial cells (HPAECs); (2) ERK1/2 inhibition will disrupt angiogenesis in vitro by repressing cell cycle progression. In mice, hyperoxia exposure transiently increased lung endothelial ERK1/2 activation at one week of life, before inhibiting it at two weeks of life. Interestingly, hyperoxia-mediated decrease in ERK1/2 activation in mice was associated with decreased angiogenesis and increased endothelial cell apoptosis. Hyperoxia also transiently activated ERK1/2 in HPAECs. ERK1/2 inhibition disrupted angiogenesis in vitro, and these effects were associated with altered levels of proteins that modulate cell cycle progression. Collectively, these findings support our hypotheses, emphasizing that the ERK1/2 pathway is a potential therapeutic target for BPD infants with decreased lung vascularization.
Genomics of Fibromuscular Dysplasia
Authors: Di Monaco Silvia , Georges Adrien , Lengelé Jean-Philippe , Vikkula Miikka , Persu Alexandre
Published: May 2018

Fibromuscular Dysplasia (FMD) is “an idiopathic, segmental, non-atherosclerotic and non-inflammatory disease of the musculature of arterial walls, leading to stenosis of small and medium-sized arteries” (Persu, et al; 2014). FMD can lead to hypertension, arterial dissections, subarachnoid haemorrhage, stroke or mesenteric ischemia. The pathophysiology of the disease remains elusive. While familial cases are rare (<5%) in contemporary FMD registries, there is evidence in favour of the existence of multiple genetic factors involved in this vascular disease. Recent collaborative efforts allowed the identification of a first genetic locus associated with FMD. This intronic variant located in the phosphatase and actin regulator 1 gene (PHACTR1) may influence the transcription activity of the endothelin-1 gene (EDN1) located nearby on chromosome 6. Interestingly, the PHACTR1 locus has also been involved in vascular hypertrophy in normal subjects, carotid dissection, migraine and coronary artery disease. National and international registries of FMD patients, with deep and harmonised phenotypic and genetic characterisation, are expected to be instrumental to improve our understanding of the genetic basis and pathophysiology of this intriguing vascular disease.
A Survey on Efforts to Evolve the Control Plane of Inter-Domain Routing
Authors: Silva Walber José Adriano , Sadok Djamel Fawzi Hadj
Published: May 2018

The Internet’s default inter-domain routing protocol is the Border Gateway Protocol (BGP). With the BGP, dozens of thousands of Autonomous Systems (ASs) exchange network layer reachability information to manage connectivity among them. The BGP was introduced in the early stages of the Internet, and although the BGP is one of the most successful protocols, new desirable features have been difficult to incorporate into the network over the decades. Thus, this paper classifies previous works to evolve the control plane of inter-domain routing into three types of approaches: brand new design; incremental improvement; inter-domain communication. The main goal of this paper is to provide an understanding of what approaches have been taken to evolve the inter-domain routing control plane. This survey also discusses why the control plane’s issues are hard to evolve and future perspectives for that topic.
Element-Weighted Neutrosophic Correlation Coefficient and Its Application in Improving CAMShift Tracker in RGBD Video
Authors: Hu Keli , Fan En , Ye Jun , Pi Jiatian , Zhao Liping , Shen Shigen
Published: May 2018

Neutrosophic set (NS) is a new branch of philosophy to deal with the origin, nature, and scope of neutralities. Many kinds of correlation coefficients and similarity measures have been proposed in neutrosophic domain. In this work, by considering that there may exist different contributions for the neutrosophic elements of T (Truth), I (Indeterminacy), and F (Falsity), a method of element-weighted neutrosophic correlation coefficient is proposed, and it is applied for improving the CAMShift tracker in RGBD (RGB-Depth) video. The concept of object seeds is proposed, and it is employed for extracting object region and calculating the depth back-projection. Each candidate seed is represented in the single-valued neutrosophic set (SVNS) domain via three membership functions, T, I, and F. Then the element-weighted neutrosophic correlation coefficient is applied for selecting robust object seeds by fusing three kinds of criteria. Moreover, the proposed correlation coefficient is applied for estimating a robust back-projection by fusing the information in both color and depth domains. Finally, for the scale adaption problem, two alternatives in the neutrosophic domain are proposed, and the corresponding correlation coefficient between the proposed alternative and the ideal one is employed for the identification of the scale. When considering challenging factors like fast motion, blur, illumination variation, deformation, and camera jitter, the experimental results revealed that the improved CAMShift tracker performs well.
TwitPersonality: Computing Personality Traits from Tweets Using Word Embeddings and Supervised Learning
Authors: Carducci Giulio , Rizzo Giuseppe , Monti Diego , Palumbo Enrico , Morisio Maurizio
Published: May 2018

We are what we do, like, and say. Numerous research efforts have been pushed towards the automatic assessment of personality dimensions relying on a set of information gathered from social media platforms such as list of friends, interests of musics and movies, endorsements and likes an individual has ever performed. Turning this information into signals and giving them as inputs to supervised learning approaches has resulted in being particularly effective and accurate in computing personality traits and types. Despite the demonstrated accuracy of these approaches, the sheer amount of information needed to put in place such a methodology and access restrictions make them unfeasible to be used in a real usage scenario. In this paper, we propose a supervised learning approach to compute personality traits by only relying on what an individual tweets about publicly. The approach segments tweets in tokens, then it learns word vector representations as embeddings that are then used to feed a supervised learner classifier. We demonstrate the effectiveness of the approach by measuring the mean squared error of the learned model using an international benchmark of Facebook status updates. We also test the transfer learning predictive power of this model with an in-house built benchmark created by twenty four panelists who performed a state-of-the-art psychological survey and we observe a good conversion of the model while analyzing their Twitter posts towards the personality traits extracted from the survey.
Key Concept Identification: A Comprehensive Analysis of Frequency and Topical Graph-Based Approaches
Authors: Aman Muhammad , Bin Md Said Abas , Jadid Abdul Kadir Said , Ullah Israr
Published: May 2018

Automatic key concept extraction from text is the main challenging task in information extraction, information retrieval and digital libraries, ontology learning, and text analysis. The statistical frequency and topical graph-based ranking are the two kinds of potentially powerful and leading unsupervised approaches in this area, devised to address the problem. To utilize the potential of these approaches and improve key concept identification, a comprehensive performance analysis of these approaches on datasets from different domains is needed. The objective of the study presented in this paper is to perform a comprehensive empirical analysis of selected frequency and topical graph-based algorithms for key concept extraction on three different datasets, to identify the major sources of error in these approaches. For experimental analysis, we have selected TF-IDF, KP-Miner and TopicRank. Three major sources of error, i.e., frequency errors, syntactical errors and semantical errors, and the factors that contribute to these errors are identified. Analysis of the results reveals that performance of the selected approaches is significantly degraded by these errors. These findings can help us develop an intelligent solution for key concept extraction in the future.
Investigation of a Monturaqui Impactite by Means of Bi-Modal X-ray and Neutron Tomography
Authors: Fedrigo Anna , Marstal Kasper , Bender Koch Christian , Andersen Dahl Vedrana , Bjorholm Dahl Anders , Lyksborg Mark , Gundlach Carsten , Ott Frédéric , Strobl Markus
Published: May 2018

X-ray and neutron tomography are applied as a bi-modal approach for the 3D characterisation of a Monturaqui impactite formed by shock metamorphism during the impact of an iron meteorite with the target rocks in the Monturaqui crater (Chile). The particular impactite exhibits structural heterogeneities on many length scales: its composition is dominated by silicate-based glassy and crystalline materials with voids and Fe/Ni-metal and oxihydroxides particles generally smaller than 1 mm in diameter. The non-destructive investigation allowed us to apply a novel bi-modal imaging approach that provides a more detailed and quantitative understanding of the structural and chemical composition compared to standard single mode imaging methods, as X-ray and neutron interaction with matter results in different attenuation coefficients with a non-linear relation. The X-ray and neutron data sets have been registered, and used for material segmentation, porosity and metallic content characterization. The bimodal data enabled the segmentation of a large number of different materials, their morphology as well as distribution in the specimen including the quantification of volume fractions. The 3D data revealed an evaporite type of material in the impactite not noticed in previous studies. The present study is exemplary in demonstrating the potential for non-destructive characterisation of key features of complex multi-phase objects such as impactites.
Relationships between Personality and Cognitive Ability: A Facet-Level Analysis
Authors: Rammstedt Beatrice , Lechner Clemens M. , Danner Daniel
Published: May 2018

A growing body of research supports the notion that cognitive abilities and personality are systematically related. However, this research has focused largely on global personality dimensions and single—often equally global—markers of cognitive ability. The present study offers a more fine-grained perspective. Specifically, it is one of the first studies to comprehensively investigate the associations between both fluid and crystallized intelligence with Big Five personality domains as well as their facets. Based on a heterogeneous sample of the adult population in Germany (N = 365), our study yielded three key findings. First, personality was more strongly related to crystallized intelligence than to fluid intelligence. This applied both to the total variance explained and to the effect sizes of most of the Big Five domains and facets. Second, facets explained a larger share of variance in both crystallized and fluid intelligence than did domains. Third, the associations of different facets of the same domain with cognitive ability differed, often quite markedly. These differential associations may substantially reduce—or even suppress—the domain-level associations. Our findings clearly attest to the added value of a facet-level perspective on the personality–cognitive ability interface. We discuss how such a fine-grained perspective can further theoretical understanding and enhance prediction.
Adhesins in Candida glabrata
Authors: Timmermans Bea , De Las Peñas Alejandro , Castaño Irene , Van Dijck Patrick
Published: May 2018

The human fungal pathogen Candida glabrata is causing more and more problems in hospitals, as this species shows an intrinsic antifungal drug resistance or rapidly becomes resistant when challenged with antifungals. C. glabrata only grows in the yeast form, so it is lacking a yeast-to-hyphae switch, which is one of the main virulence factors of C. albicans. An important virulence factor of C. glabrata is its capacity to strongly adhere to many different substrates. To achieve this, C. glabrata expresses a large number of adhesin-encoding genes and genome comparisons with closely related species, including the non-pathogenic S. cerevisiae, which revealed a correlation between the number of adhesin-encoding genes and pathogenicity. The adhesins are involved in the first steps during an infection; they are the first point of contact with the host. For several of these adhesins, their importance in adherence to different substrates and subsequent biofilm formation was demonstrated in vitro or in vivo. In this review, we provide an overview of the role of C. glabrata adhesins during adhesion and biofilm formation both, under in vitro and in vivo conditions.
Judicialization and Its Effects: Experiments as a Way Forward
Authors: Hevron Parker
Published: May 2018

Law and courts play a larger role in American policymaking than in similar countries—and a larger role than ever before in American politics. However, systematic efforts to evaluate the effects of judicialized policymaking are consistently plagued by problems of causal inference. Experiments offer a way forward. Causal claims by public law scholars are often undercut by validity difficulties that are avoidable if scholars engaging in observational research incorporate the tenets of experiments in their research designs, as well as if more public law scholars attempted to isolate the effects of judicialization in controlled settings, such as survey or laboratory experiments. An original survey experiment on the effects of media reporting on tort reform suggests that experiments have much to offer public law scholars. Despite certain challenges in implementation, experiments and observational research based on experiments provide a promising path for assessing the varied—and important—effects of judicialized policymaking.
Stability of GO Modified by Different Dispersants in Cement Paste and Its Related Mechanism
Authors: Long Wu-Jian , Fang Changle , Wei Jingjie , Li Haodao
Published: May 2018

Graphene oxide (GO) is a potential material to be used as a nano-reinforcement in cement matrix. However, a prerequisite for GO to fulfill its function in the cement matrix is homogeneous dispersion. In this study, the effects of three different dispersing agents (DAs), including polycarboxylate-based high range water reducer (P-HRWR), naphthalene-based high range water reducer (N-HRWR), and air entraining agent (AEA) on the dispersion of GO in aqueous solution, simulated concrete pore solution (SCPS), and suspension of cement pastes were sequentially investigated. Results showed that the dispersion effect of GO in aqueous solutions was improved with different DAs. However, the homogeneous dispersion of GO in aqueous solution re-agglomerated in SCPS and suspension of cement pastes. It was concluded that as the cement content and pH of aqueous solutions increased, GOs re-agglomerated and precipitated in an alkaline solution. A possible mechanism was proposed in this study and it was believed that electrostatic interactions and steric hindrance provided by the P-HRWR further made GOs stable in aqueous solutions. The ions and pH of cement pastes increased with the increasing amount of cement, which caused the separation of P-HRWR from GOs. Therefore, GOs were re-agglomerated and absorbed on the surface of the cement particles, resulting in GOs sedimentation.
Ultraviolet Laser Lithography of Titania Photonic Crystals for Terahertz-Wave Modulation
Authors: Kirihara Soshu , Nonaka Koki , Kisanuki Shoichiro , Nozaki Hirotoshi , Sakaguchi Keito
Published: May 2018

Three-dimensional (3D) microphotonic crystals with a diamond structure composed of titania microlattices were fabricated using ultraviolet laser lithography, and the bandgap properties in the terahertz (THz) electromagnetic-wave frequency region were investigated. An acrylic resin paste with titania fine particle dispersions was used as the raw material for additive manufacturing. By scanning a spread paste surface with an ultraviolet laser beam, two-dimensional solid patterns were dewaxed and sintered. Subsequently, 3D structures with a relative density of 97% were created via layer lamination and joining. A titania diamond lattice with a lattice constant density of 240 µm was obtained. The properties of the electromagnetic wave were measured using a THz time-domain spectrometer. In the transmission spectra for the Γ-X <100> direction, a forbidden band was observed from 0.26 THz to 0.44 THz. The frequency range of the bandgap agreed well with calculated results obtained using the plane–wave expansion method. Additionally, results of a simulation via transmission-line modeling indicated that a localized mode can be obtained by introducing a plane defect between twinned diamond lattice structures.
Microstructural Evolution in High-Strain-Rate Deformation of Ti-5Al-5Mo-5V-1Cr-1Fe Alloy
Authors: Ran Chun , Chen Pengwan , Sheng Zemin , Li Jingbo , Zhang Wangfeng
Published: May 2018

To study the microstructural evolution in high-strain-rate shear deformation of Ti-5Al-5Mo-5V-1Cr-1Fe (Ti-55511) alloy, a series of forced shear tests of hat-shaped specimens have been conducted using a split Hopkinson pressure bar combined with the “strain-frozen” technique. A localized shear band is induced in Ti-55511 alloy in these tests. The experimental results demonstrate that the flow stress in hat-shaped specimens remains constant (about 600 MPa) and is independent of punching depth. The width of the adiabatic shear band increases with increasing punching depth and tends to saturate at 30 μm, and the estimation of the adiabatic shear band (ASB) width in hat-shaped (HS) specimens has been modified. Relying on the experimental results, thermal softening has a minor effect on the onset of the adiabatic shear band and dynamic recrystallization formation, and the nucleation mechanism for dynamic recrystallization is strain-induced boundary migration and subgrain rotation and coalescence. In addition, we suggest the concept of adhesive fracture as the dynamic failure mechanism for Ti-55511 alloy.
Photovoltaic Performance Enhancement of Silicon Solar Cells Based on Combined Ratios of Three Species of Europium-Doped Phosphors
Authors: Ho Wen-Jeng , You Bang-Jin , Liu Jheng-Jie , Bai Wen-Bin , Syu Hong-Jhang , Lin Ching-Fuh
Published: May 2018

This paper presents a scheme for the enhancement of silicon solar cells in terms of luminescent emission band and photovoltaic performance. The proposed devices are coated with an luminescent down-shifting (LDS) layer comprising three species of europium (Eu)-doped phosphors mixed within a silicate film (SiO2) using a spin-on film deposition. The three species of phosphor were mixed at ratios of 0.5:1:1.5, 1:1:1, or 1.5:1:0.5 in weight percentage (wt %). The total quantity of Eu-doped phosphors in the silicate solution was fixed at 3 wt %. The emission wavelengths of the Eu-doped phosphors were as follows: 518 nm (specie-A), 551 nm (specie-B), and 609 nm (specie-C). We examined the extended luminescent emission bands via photoluminescence measurements at room temperature. Closely matching the luminescent emission band to the high responsivity band of the silicon semiconductor resulted in good photovoltaic performance. Impressive improvements in efficiency were observed in all three samples: 0.5:1:1.5 (20.43%), 1:1:1 (19.67%), 1.5:1:0.5 (16.81%), compared to the control with a layer of pure SiO2 (13.80%).
Polysulfobetaines in Aqueous Solution and in Thin Film Geometry
Authors: Niebuur Bart-Jan , Puchmayr Jonas , Herold Christian , Kreuzer Lucas P. , Hildebrand Viet , Müller-Buschbaum Peter , Laschewsky André , Papadakis Christine M.
Published: May 2018

Polysulfobetaines in aqueous solution show upper critical solution temperature (UCST) behavior. We investigate here the representative of this class of materials, poly (N,N-dimethyl-N-(3-methacrylamidopropyl) ammonio propane sulfonate) (PSPP), with respect to: (i) the dynamics in aqueous solution above the cloud point as function of NaBr concentration; and (ii) the swelling behavior of thin films in water vapor as function of the initial film thickness. For PSPP solutions with a concentration of 5 wt.%, the temperature dependence of the intensity autocorrelation functions is measured with dynamic light scattering as function of molar mass and NaBr concentration (0–8 mM). We found a scaling of behavior for the scattered intensity and dynamic correlation length. The resulting spinodal temperatures showed a maximum at a certain (small) NaBr concentration, which is similar to the behavior of the cloud points measured previously by turbidimetry. The critical exponent of susceptibility depends on NaBr concentration, with a minimum value where the spinodal temperature is maximum and a trend towards the mean-field value of unity with increasing NaBr concentration. In contrast, the critical exponent of the correlation length does not depend on NaBr concentration and is lower than the value of 0.5 predicted by mean-field theory. For PSPP thin films, the swelling behavior was found to depend on film thickness. A film thickness of about 100 nm turns out to be the optimum thickness needed to obtain fast hydration with H2O.
Generalized Hyers-Ulam Stability of Trigonometric Functional Equations
Authors: Elqorachi Elhoucien , Rassias Michael Th.
Published: May 2018

In the present paper we study the generalized Hyers–Ulam stability of the generalized trigonometric functional equations f ( x y ) + μ ( y ) f ( x σ ( y ) ) = 2 f ( x ) g ( y ) + 2 h ( y ) , x , y ∈ S ; f ( x y ) + μ ( y ) f ( x σ ( y ) ) = 2 f ( y ) g ( x ) + 2 h ( x ) , x , y ∈ S , where S is a semigroup, σ : S ⟶ S is a involutive morphism, and μ : S ⟶ C is a multiplicative function such that μ ( x σ ( x ) ) = 1 for all x ∈ S . As an application, we establish the generalized Hyers–Ulam stability theorem on amenable monoids and when σ is an involutive automorphism of S.
Association of Monoamine Oxidase A (MAOA) Gene uVNTR and rs6323 Polymorphisms with Attention Deficit and Hyperactivity Disorder in Korean Children
Authors: Hwang In Wook , Lim Myung Ho , Kwon Ho Jang , Jin Han Jun
Published: May 2018

Objective: Attention deficit hyperactivity disorder (ADHD) is a common neurodevelopmental disorder. The genetic cause of ADHD is still unclear, but the dopaminergic, serotonergic, and noradrenergic pathways have shown a strong association. In particular, monoamine oxidase A (MAOA) plays an important role in the catabolism of these neurotransmitters, suggesting that the MAOA gene is associated with ADHD. Therefore, we evaluated the relationship between the MAOA gene polymorphisms (uVNTR and rs6323) and ADHD. Materials and methods: We collected a total of 472 Korean children (150 ADHD cases and 322 controls) using the Korean version of the Dupaul Attention Deficit Hyperactivity Disorder Rating Scales (K-ARS). Genotyping was performed by PCR and PCR-RFLP. The Behavior Assessment System for Children Second Edition (BASC-2) was used to evaluate the problem behaviors within ADHD children. Results: We observed significant associations between the rs6323 and ADHD in girls (p < 0.05) and the TT genotype was observed as a protective factor against ADHD in the recessive model (OR 0.31, 95% CI 0.100–0.950, p = 0.022). The 3.5R-G haplotype showed a significant association in ADHD boys (p = 0.043). The analysis of subtype also revealed that the 4.5R allele of uVNTR was a risk factor for the development of ADHD in the combined symptom among girls (OR 1.87, 95% CI 1.014–3.453, p = 0.031). In the BASC-2 analysis, the MAOA uVNTR polymorphism was associated with activities of daily living in ADHD boys (p = 0.017). Conclusion: These results suggest the importance of the MAOA gene polymorphisms in the development of ADHD in Korean children. A larger sample set and functional studies are required to further elucidate of our findings.
Investigation of Daphnia magna Sub-Lethal Exposure to Organophosphate Esters in the Presence of Dissolved Organic Matter Using 1H NMR-Based Metabolomics
Authors: Kovacevic Vera , Simpson André J. , Simpson Myrna J.
Published: May 2018

Organophosphate esters (OPEs) are frequently detected in aquatic environments. Hydrophobic OPEs with high octanol-water partition coefficients (Log KOW) will likely sorb to dissolved organic matter (DOM) and consequently alter OPE bioavailability and sub-lethal toxicity. 1H nuclear magnetic resonance (NMR)-based metabolomics was used to evaluate how DOM (5 mg organic carbon/L) alters the metabolic response of Daphnia magna exposed to sub-lethal concentrations of three individual OPEs with varying hydrophobicity. D. magna exposed to the hydrophilic contaminant (Log KOW = 1.43) tris(2-chloroethyl) phosphate (TCEP) did not have substantial metabolic changes and DOM did not alter the metabolic response. There were significant increases in amino acids and a decrease in glucose from exposure to the hydrophobic contaminant (Log KOW = 3.65) tris(2-butoxyethyl) phosphate (TBOEP) which DOM did not mitigate, likely due to the high sub-lethal toxicity of TBOEP. Exposure to DOM and the hydrophobic contaminant (Log KOW = 4.76) triphenyl phosphate (TPhP) resulted in a unique metabolic response which was unlike TPhP only exposure, perhaps because DOM may be an additional stressor with TPhP exposure. Therefore, Log KOW values may not always predict how sub-lethal contaminant toxicity will change with DOM and there should be more consideration to incorporate DOM in sub-lethal ecotoxicology testing.
Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking
Authors: Claveau Sandra , Bertrand Jean-Rémi , Treussart François
Published: May 2018

Diamond nanocrystals smaller than 100 nm (nanodiamonds) are now recognized to be highly biocompatible. They can be made fluorescent with perfect photostability by creating nitrogen-vacancy (NV) color centers in the diamond lattice. The resulting fluorescent nanodiamonds (FND) have been used since the late 2000s as fluorescent probes for short- or long-term analysis. FND can be used both at the subcellular scale and the single cell scale. Their limited sub-diffraction size allows them to track intracellular processes with high spatio-temporal resolution and high contrast from the surrounding environment. FND can also track the fate of therapeutic compounds or whole cells in the organs of an organism. This review presents examples of FND applications (1) for intra and intercellular molecular processes sensing, also introducing the different potential biosensing applications based on the optically detectable electron spin resonance of NV− centers; and (2) for tracking, firstly, FND themselves to determine their biodistribution, and secondly, using FND as cell tracking probes for diagnosis or follow-up purposes in oncology and regenerative medicine.
Motion Constraints and Vanishing Point Aided Land Vehicle Navigation
Authors: Liu Zhenbo , El-Sheimy Naser , Yu Chunyang , Qin Yongyuan
Published: May 2018

In the typical Inertial Navigation System (INS)/ Global Navigation Satellite System (GNSS) setup for ground vehicle navigation, measures should be taken to maintain the performance when there are GNSS signal outages. Usually, aiding sensors are utilized to reduce the INS drift. A full motion constraint model is developed allowing the online calibration of INS frame with respect to (w.r.t) the motion frame. To obtain better heading and lateral positioning performance, we propose to use of vanishing point (VP) observations of parallel lane markings from a single forward-looking camera to aid the INS. In the VP module, the relative attitude of the camera w.r.t the road frame is derived from the VP coordinates. The state-space model is developed with augmented vertical attitude error state. Finally, the VP module is added to a modified motion constrains module in the Extended Kalman filter (EKF) framework. Simulations and real-world experiments have shown the validity of VP-based method and improved heading and cross-track position accuracy compared with the solution without VP. The proposed method can work jointly with conventional visual odometry to aid INS for better accuracy and robustness.
Optimization of Ultrasound-Assisted Extraction and Structural Characterization of the Polysaccharide from Pumpkin (Cucurbita moschata) Seeds
Authors: Wang Libo , Cheng Long , Liu Fangcheng , Li Tengfei , Yu Zeyuan , Xu Yaqin , Yang Yu
Published: May 2018

In the present study, ultrasound-assisted extraction (UAE) of crude polysaccharides (PSP) from pumpkin seeds was optimized by response surface method (RSM). The polysaccharide yield (2.29 ± 0.14%), which agreed closely with the theoretical predicted value 2.40%, was obtained under the optimal extraction conditions: extraction time 24 min, extraction temperature 50 °C, ultrasonic power 347 W, and liquid to solid ratio 23 mL/g. After further purification by two-step column chromatography, a novel polysaccharide (PSP-1) was isolated from pumpkin seeds. PSP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.00:4.26:5.78 with molecular weight of 3728 g/mol. 1D and 2D NMR spectroscopy analysis revealed that the backbone of PSP-1 was mainly formed by β→6)-β-d-Galp-(1→, →6)-α-d-Glcp-(1→, and →3,6)-β-d-Manp-(1→ with branching at O-3 and O-6 of →3,6)-β-d-Manp-(1→. Branch linkages were composed of α-d-Glcp-(1→ and →4)-α-d-Galp-(1→.
β-Phenylalanine Ester Synthesis from Stable β-Keto Ester Substrate Using Engineered ω-Transaminases
Authors: Buß Oliver , Voss Moritz , Delavault André , Gorenflo Pascal , Syldatk Christoph , Bornscheuer Uwe , Rudat Jens
Published: May 2018

The successful synthesis of chiral amines from ketones using ω-transaminases has been shown in many cases in the last two decades. In contrast, the amination of β-keto acids is a special and relatively new challenge, as they decompose easily in aqueous solution. To avoid this, transamination of the more stable β-keto esters would be an interesting alternative. For this reason, ω-transaminases were tested in this study, which enabled the transamination of the β-keto ester substrate ethyl benzoylacetate. Therefore, a ω-transaminase library was screened using a coloring o-xylylenediamine assay. The ω-transaminase mutants 3FCR_4M and ATA117 11Rd show great potential for further engineering experiments aiming at the synthesis of chiral (S)- and (R)-β-phenylalanine esters. This alternative approach resulted in the conversion of 32% and 13% for the (S)- and (R)-enantiomer, respectively. Furthermore, the (S)-β-phenylalanine ethyl ester was isolated by performing a semi-preparative synthesis.
Novel Imidazole Aldoximes with Broad-Spectrum Antimicrobial Potency against Multidrug Resistant Gram-Negative Bacteria
Authors: Skočibušić Mirjana , Odžak Renata , Ramić Alma , Smolić Tomislav , Hrenar Tomica , Primožič Ines
Published: May 2018

In the search for a new class of potential antimicrobial agents, five novel N-substituted imidazole 2-aldoximes and their six quaternary salts were evaluated. The antimicrobial activity was assessed against a panel of representative Gram-positive and Gram-negative bacteria, including multidrug resistant bacteria. All compounds demonstrated potent in vitro activity against the tested microorganisms, with MIC values ranging from 6.25 to 50.0 μg/mL. Among the tested compounds, two quaternary compounds (N-but-3-enyl- and meta- (10) or para- N-chlorobenzyl (11) imidazolium 2-aldoximes) displayed the most potent and broad-spectrum activity against both Gram-positive and Gram-negative bacterial strains. The broth microdilution assay was also used to investigate the antiresistance efficacy of the both most active compounds against a set of Enterobacteriaceae isolates carried a multiple extended-spectrum β-lactamases (ESBLs) in comparison to eight clinically relevant antibiotics. N-but-3-enyl-N-meta-chlorobenzyl imidazolium 2-aldoxime was found to possess promising antiresistance efficacy against a wide range of β-lactamases producing strains (MIC 2.0 to 16.0 μg/mL). Best results for that compound were obtained against Escherichia coli and Enterobacter cloacae producing multiple β-lactamases form A and C molecular classes, which were 32- and 128-fold more potent than ceftazidime and cefotaxime, respectively. To visualize the results, principal component analysis was used as an additional classification tool. The mixture of ceftazidime and compound 10 (3 μg:2 μg) showed a strong activity and lower the necessary amount (up to 40-fold) of 10 against five of ESBL-producing isolates (MIC ≤ 1 µg/mL).
Echinacoside, an Inestimable Natural Product in Treatment of Neurological and other Disorders
Authors: Liu Jingjing , Yang Lingling , Dong Yanhong , Zhang Bo , Ma Xueqin
Published: May 2018

Echinacoside (ECH), a natural phenylethanoid glycoside, was first isolated from Echinacea angustifolia DC. (Compositae) sixty years ago. It was found to possess numerous pharmacologically beneficial activities for human health, especially the neuroprotective and cardiovascular effects. Although ECH showed promising potential for treatment of Parkinson’s and Alzheimer’s diseases, some important issues arose. These included the identification of active metabolites as having poor bioavailability in prototype form, the definite molecular signal pathways or targets of ECH with the above effects, and limited reliable clinical trials. Thus, it remains unresolved as to whether scientific research can reasonably make use of this natural compound. A systematic summary and knowledge of future prospects are necessary to facilitate further studies for this natural product. The present review generalizes and analyzes the current knowledge on ECH, including its broad distribution, different preparation technologies, poor pharmacokinetics and kinds of therapeutic uses, and the future perspectives of its potential application.
Antioxidants from the Brown Alga Dictyopteris undulata
Authors: Kumagai Momochika , Nishikawa Keisuke , Matsuura Hiroshi , Umezawa Taiki , Matsuda Fuyuhiko , Okino Tatsufumi
Published: May 2018

An investigation of anti-oxidative compounds from the brown alga Dictyopteris undulata has led to the isolation and identification of isozonarol, isozonarone, chromazonarol, zonaroic acid and isozonaroic acid. Their structures were identified by comparison of MS and NMR spectra. Full NMR assignment and absolute configuration of isozonaroic acid are described. Isozonarol showed the most potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity among the compounds isolated.
In Vitro Estrogenic and Breast Cancer Inhibitory Activities of Chemical Constituents Isolated from Rheum undulatum L.
Authors: Lee Dahae , Park SeonJu , Choi Sungyoul , Kim Seung , Kang Ki
Published: May 2018

We investigated the estrogenic and breast cancer inhibitory activities of chemical constituents isolated from Rhei undulati Rhizoma (roots of Rheum undulatum L.), which is used as a laxative, an anti-inflammatory, and an anti-blood stagnation agent. Estrogen-like activity was studied using the well characterized E-screen assay in estrogen receptor (ER)-positive MCF-7 cells. The mechanism underlying the breast cancer inhibitory activity of the compounds was studied using human ER-negative MDA-MB-231 and ER-positive MCF-7 cells. The activation of apoptosis pathway-related proteins was investigated by western blotting, using extracts of R. undulatum prepared in three solvent conditions (EX1, EX2, and EX3). The R. undulatum chemical constituents (compounds 1–3) showed estrogen-like activity in the concentration range of 10 to 50 μM, by increasing the proliferation of human ER-positive MCF-7 cells. These effects were attenuated by co-treatment with 100 nM fulvestrant, an ER antagonist. Compounds 1–3 decreased the viability of MCF-7 cells in a concentration-dependent manner. Compounds 1 (aloe emodin) and 2 (rhapontigenin) induced mitochondria-independent apoptosis by activating the caspase-8 pathway, whereas the cytotoxic effect of compound 3 (chrysophanol 1-O-β-d-glucopyranoside) was mediated through the mitochondria-dependent apoptotic pathway.
Synthesis and Insecticidal Activity of Mesoionic Pyrido[1,2-α]pyrimidinone Derivatives Containing a Neonicotinoid Moiety
Authors: Pan Jianke , Yu Lu , Liu Dengyue , Hu Deyu
Published: May 2018

Mesoionic pyrido[1,2-α]pyrimidinone derivatives containing a neonicotinoid moiety were designed, synthesized, and evaluated for their insecticidal activity. Some of the title compounds showed remarkable insecticidal properties against Aphis craccivora. Compound I13 exhibited satisfactory insecticidal activity against A. craccivora. Meanwhile, label-free proteomics analysis of compound I13 treatment identified a total of 821 proteins. Of these, 35 proteins were up-regulated, whereas 108 proteins were down-regulated. Differential expressions of these proteins reflected a change in cellular structure and metabolism.
Targeting Protein Quality Control Mechanisms by Natural Products to Promote Healthy Ageing
Authors: Wedel Sophia , Manola Maria , Cavinato Maria , Trougakos Ioannis P. , Jansen-Dürr Pidder
Published: May 2018

Organismal ageing is associated with increased chance of morbidity or mortality and it is driven by diverse molecular pathways that are affected by both environmental and genetic factors. The progression of ageing correlates with the gradual accumulation of stressors and damaged biomolecules due to the time-dependent decline of stress resistance and functional capacity, which eventually compromise cellular homeodynamics. As protein machines carry out the majority of cellular functions, proteome quality control is critical for cellular functionality and is carried out through the curating activity of the proteostasis network (PN). Key components of the PN are the two main degradation machineries, namely the ubiquitin-proteasome and autophagy-lysosome pathways along with several stress-responsive pathways, such as that of nuclear factor erythroid 2-related factor 2 (Nrf2), which mobilises cytoprotective genomic responses against oxidative and/or xenobiotic damage. Reportedly, genetic or dietary interventions that activate components of the PN delay ageing in evolutionarily diverse organisms. Natural products (extracts or pure compounds) represent an extraordinary inventory of highly diverse structural scaffolds that offer promising activities towards meeting the challenge of increasing healthspan and/or delaying ageing (e.g., spermidine, quercetin or sulforaphane). Herein, we review those natural compounds that have been found to activate proteostatic and/or anti-stress cellular responses and hence have the potential to delay cellular senescence and/or in vivo ageing.