bluuebunny/crossref_metadata_2025 huggingface dataset:
Classification of Drilled Lithology in Real-Time Using Deep Learning with Online Calibration
Authors: Arnø Mikkel Leite , Godhavn John-Morten , Aamo Ole Morten
Published: November 2021

Decision making to optimize the drilling operation is based on a variety of factors, among them real-time interpretation of drilled lithology. Because logging while drilling (LWD) tools are placed some meters above the bit, mechanical drilling parameters are the earliest indicators, although they are difficult to interpret accurately. This paper presents a novel deep learning methodology using mechanical drilling parameters for lithology classification. A cascade of deep neural networks (DNNs) are trained on historical data from wells on a field operated by Equinor. Rather than an end-to-end approach, the drilling parameters are used to estimate LWD sensor readings in an intermediate step using the first DNNs. This allows continuous updates of the models during operation using delayed LWD data. The second DNN takes the virtual LWD estimates as input to predict currently drilled lithology, similar to manual expert interpretation of logs. This configuration takes into account case-dependent [mud, bottomhole assembly (BHA), wellbore geometry] and time-varying (bit wear, wellbore friction) relationships between drilling parameters and LWD readings while assuming a constant rule when using LWD data to classify lithology. Upon completion of training and validation, the system is tested on a separate, unseen wellbore, for which results are presented. Visualizations for true lithology alongside the estimates are given, along with confusion matrices and model accuracy. The system achieves high accuracy on the test set and presents low confusion between classes, meaning that it distinguishes well between the lithologies present in the wellbore. It can be seen that the borders between successive layers of lithology are detected rapidly, which is crucial seen from an optimization standpoint, so the driller may immediately adjust accordingly. It shows promise as an advisory system, capable of accurately classifying currently drilled lithology by continuously adapting to changing downhole conditions. Although we cannot expect perfect estimates of lithology purely based on drilling parameters, we can obtain a preliminary map of the subsurface this way. This novel configuration gives a real-time interpretation of the currently drilled lithology. Thus, the drilling operation can be improved through early information and prompt drilling parameter adjustments to accommodate the current drilling environment.
Quantitative Hydraulic-Fracture-Geometry Characterization with Low-Frequency Distributed-Acoustic-Sensing Strain Data: Fracture-Height Sensitivity and Field Applications
Authors: Liu Yongzan , Jin Ge , Wu Kan , Moridis George
Published: November 2021

Low-frequency distributed acoustic sensing (LF-DAS) has been used for hydraulic fracture monitoring and characterization. Large amounts of DAS data have been acquired across different formations. The low-frequency components of DAS data are highly sensitive to mechanical strain changes. Forward geomechanical modeling has been the focus of current research efforts to better understand LF-DAS signals. Moreover, LF-DAS provides the opportunity to quantify fracture geometry. Recently, Liu et al. (2021a, 2021b) proposed an inversion algorithm to estimate hydraulic-fracture width using LF-DAS data measured during multifracture propagation. The LF-DAS strain data are linked to the fracture widths through a forward model developed based on the displacement discontinuity method. In this study, we first investigated the impacts of fracture height on the inversion results through a numerical case with a four-cluster completion design. Then we discussed how to estimate the fracture height based on the inversion results. Finally, we applied the inversion algorithm to two field examples.

The inverted widths are not sensitive to the fracture height. In the synthetic case, the maximum relative error is less than 10% even when the fracture height is two times the true value. After obtaining the fracture width, the fracture height can be estimated by matching the true strain data under various heights with a strong smooth weight. The error between the calculated strain and true strain decreases as the height approaches the true value. In the two field examples, the temporal evolutions of the width summation of all fractures and the width of each fracture show consistent behaviors with the field LF-DAS measurements. The calculated strain data from the forward model match well with the field LF-DAS strain data. The results demonstrate the robustness and accuracy of the proposed inversion algorithm.
Synthesis of Asphalt Nanoparticles and Their Effects on Drilling Fluid Properties and Shale Dispersion
Authors: Xionghu Z. , Egwu S. B. , Jingen D. , Liujie M. , Xiangru J.
Published: November 2021

Asphalt nanoparticles (ANs) were developed by synthesizing asphalt powders with chloroacetic acid (ClCH2COOH). The objective of this synthesis was to develop engineered ANs with a cationic point capable of adsorbing on the net negatively charged clay platelets, thereby improving drilling fluid functionality and pore-plugging performance, reducing shale dispersion, and ultimately enhancing shale stability. Tests carried out to study the performance of the synthesized ANs include particle size analysis, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy, drilling fluid rheology, and filtration rate and shale dispersion tests. FT-IR spectrum results confirming the occurrence of a chemical reaction between asphalt and ClCH2COOH showed a shift in NH vibration from 3,439.95 cm−1 (before synthesis) to 3,435.05 cm−1 (after synthesis). Based on particle size analysis, an average particle size diameter of 92.9 nm was observed, suggesting the tendency of ANs to invade and seal nanopore spaces. The shape of ANs ranged from spherical to irregular, because intercalated structures were observed from the scanning electron microscopic analysis on the interaction between ANs and sodium bentonite (Na-Bent). An increase in attracting force between the Na-Bent particles caused by the adsorption of ANs cationic point on bentonite clay particles led to an increase in drilling fluid rheological properties as the ANs %w/v increased. The drilling fluid filtration rate was, however, not significantly affected by the %w/v increase in ANs because results indicated slight decrease in fluid loss when compared with the base mud (BM). According to the shale dispersion test, the shale cuttings percentage recovery of the 2%w/v ANs sample was 76.5%, owing to the decrease in fluid-rock interaction caused by ionic adsorption and encapsulation of shale surfaces by the ANs. Experimental results from this investigation indicate that the likely mechanisms of the effect of ANs on shale formations would be sealing off nanopore spaces in formations because of its ultratiny particle size; adsorption of the net negatively charged shale cuttings by the ANs cationic point, thereby reducing drilling cuttings dispersion; and improving hole-cleaning performance due to its effect on the drilling fluid rheological properties.
Integrated PCR testing and extended window contact tracing system for COVID-19 to improve comprehensiveness and speed
Authors: Raymenants Joren , Geenen Caspar , Thibaut Jonathan , Gorissen Sarah , Nelissen Klaas , André Emmanuel
Published: November 2021

Testing and contact tracing are standard tools for controlling the spread of COVID-191. Their effectiveness hinges on a sequence of processes encompassing testing coverage and timeliness, testing quality and speed of reporting, contact tracing speed and comprehensiveness and compliance with advice given2–6. We optimized this sequence of processes in the context of a public health program targeting around 33,000 higher education students through a combination of low barrier PCR testing with rapid turn-around-time, close integration of testing and tracing teams and IT infrastructure, community engagement and the implementation of bidirectional contact tracing by extending the contact tracing window from 2 to 7 days before symptom onset or test of the index case. We anticipate this combined intervention to help improve epidemic control.
Duplex RTqPCRs for detection and relative quantification of SARS-CoV-2 variants of concern (VOC)
Authors: Carcereny Albert , Garcia-Pedemonte David , Bosch Albert , Pintó Rosa M , Guix Susana
Published: November 2021

During the evolution of the SARS-CoV-2 pandemic, new variants of the virus have emerged and spread worldwide. The increased transmissibility and proclivity of some of these variants to cause more serious disease threatens public health responses against the virus, and they are classified as variants of concern (Variants of Concern, VOCs). While Next-Generation-Sequencing (NGS) is the gold standard to identify VOC, it cannot always be rapidly implemented in some settings to provide information as an early warning tool. Duplex quantitative real time RTqPCR assays offer a sensitive and easy-to-use tool to detect, discriminate, and estimate relative proportions of SARS-CoV-2 variants containing VOC-specific signature mutations from variants lacking it, using allelic discrimination probes. We developed three multiplexed RTqPCR assays that can detect Beta (B.1.351), Gamma (P.1) and Delta (B.1.617.2) VOCs by targeting 22281_22289DelCTTTACTTG, 28262_28263InsAACA and 22029_22034DelAGTTCA deletions/insertions in their genomes, respectively. Beta and Delta deletions are mapped to the S gene (residues 241/243 and 157/158, respectively), while Gamma insertion is located between the end of ORF8 and the beginning of N gene. Our specific duplex RTqPCR assays have been adapted from a previously designed duplex RTqPCR assay used to estimate the relative proportion of genomes containing 21765-21770DelTACATG mutation affecting residues HV69/70, a signature mutation of Alpha VOC (Carcereny et al., 2021). All duplex RTqPCR assays targeting signature mutations of VOCs may be used as a complementary tool to NGS for rapid variant tracking and surveillance in wastewater-based epidemiology.
Protocol for the study of hepatic bilirubin uptake in the isolated perfused rat liver
Authors: Stebel Marco , Medic Nevenka , Pelizzo Paola , Sist Paola , Tramer Federica , Passamonti Sabina
Published: November 2021

We present a protocol for the study of bilirubin uptake in the isolated and perfused rat liver. The liver is perfused with an albumin-free saline buffered solution supplemented with glucose, pyruvate and lactate, in the absence of oxygen, at a physiologically low flow rate. Fractions of the venous effluent are collected and analyzed for bilirubin, bilirubin glucuronide and biomarkers of liver integrity. The liver preparation is viable and intact for 1 h after isolation from the general circulation, with constant levels of both bilirubin and bilirubin glucuronide (< 2 nM) in the effluent. Up to 12 boli of 10 nmol bilirubin can be sequentially injected into the portal vein without and with molecules that target sinusoidal membrane transporters of organic anions. Selective inhibition of bilirubin or bilirubin glucuronide uptake is detected as transient peaks in the effluent (Cmax up 6 to 60 nM). This protocol allows collecting repeated observations in the same liver, thus reducing the animal number by a factor of 10.
Maternal Vaginal Fluids Play A Major Role In The Colonization of The Neonatal Intestinal Microbiota
Authors: Tang Chen , Zhang Xueqin , Xie Jingxian , Zeng Lingyu , Xin Yuntian , Zhang Jie , Lin Yi , Xiao Yunshan , Wu Quanfeng , Shen Heqing
Published: November 2021

Background: Caesarean delivery (CD) is associated with newborns’ health risks due to the blocking of microbiome transfer. To understand the vertical bacterial seeding and reduce CD disadvantages, microbiome transmissions via anal and genital routes were investigated, and the efficiency of vaginal fluid swabbing treatment was evaluated using 16s rDNA sequencing-based techniques.Results: Pregnant women were recruited in the Women and Children’s Hospital, School of Medicine, Xiamen University from June 1st to August 15th, 2017. Maternal faeces (n = 26), maternal vaginal fluids (n = 26), and neonatal transitional stools (n = 26) were collected, while the participants underwent natural delivery (ND) (n = 6), CD (n = 4) and CD with vaginal fluid swabbing (CS) on their newborns (n = 16). 26 mothers with the median age 26.50 (25.00-27.25) years showed no substantial clinical differences. The newborns’ gut microbiota altered among ND, CD and CS, and clustered into two groups (PERMANOVA P < 0.01) of swabbing and no-swabbing exposure. Gut colonization of ND babies majorly originated from maternal vaginal microbes (PERMANOVA P = 0.08), no vertical transmission was observed via anal route in any group. The vaginal transfer partially occurred by swabbing, in which the phyla Firmicutes and Proteobacteria and the genera Lactobacillus, Bacteroides, and Escherichia-Shigella in newborns were similar to their mothers. The recovered taxa predicted KEGG functions of biosynthesis, metabolism, and DNA replication and repair with benefits of low risks of digestive, cardiovascular, and immune diseases.Conclusions: The newborn’s gut microbiota is mainly shaped by maternal vaginal microbiota, and aberrant colonization initiated by CD is partly mitigated by the swabbing treatment.
Ustekinumab Trough Levels Predicting Laboratory and Endoscopic Remission in Patients with Crohn’s Disease
Authors: Hirayana Hisashi , Morita Yasuhiro , Imai Takayuki , Takahashi Kenichiro , Yoshida Atsushi , Bamba Shigeki , Inatomi Osamu , Andoh Akira
Published: November 2021

Backgrounds: Optimal concentration of ustekinumab (UST) predicting endoscopic remission has not been fully investigated in Crohn’s disease (CD). We aimed to identify the optimal UST trough levels predicting clinical, laboratory and endoscopic remission in CD patients. Methods: Twenty-eight patients with CD were enrolled and investigated (27 patients by enteroscopy and 1 by colonoscopy). The endoscopic activity was assessed using the modified Rutgeerts scoring system. Serum UST trough levels and anti-UST antibodies (AUAs) levels were determined by in-house immunoassays. Results: Endoscopic activity was negatively correlated with serum UST trough levels (Spearman’s rank correlation coefficient (ρ) = -0.66, P=0.0001) and serum albumin levels (ρ=-0.60, P=0.0007). The endoscopic activity was positively and significantly correlated with CRP (ρ=0.59, P=0.0009) and ESR (ρ=0.44, P=0.033). There was no significant association between the endoscopic score and AUA levels and/or Crohn’s disease activity index (CDAI). Serum UST trough levels and albumin levels were significantly higher in the endoscopic remission group (scores of 0 and 1) than in the non-endoscopic remission group (UST trough, mean 3.3 vs. 1.8μg/mL). No significant difference was observed in AUAs between the endoscopic remission and non-remission groups. Receiver operation curve (ROC) analysis revealed that the optimal cutoff value of UST trough levels predicting normal CRP and serum albumin levels was 1.7μg/mL for each, and the optimal cutoff value predicting endoscopic remission was 2.0μg/mL (AUC: 0.80, 95%-CI: 0.64-0.96). Conclusion: Endoscopic remission requires higher UST trough levels than those needed to normalize CRP and serum albumin levels.
Structure Control of Novel Hierarchical Porous Carbon Material and its Adsorption Properties
Authors: Cai Li-Feng , Zhan Jie-Min , Liang Jie , Yang Lei , Yin Jie
Published: November 2021

Novel hierarchical porous carbon materials (HPCs) were fabricated via a reactive template-induced in situ hypercrosslinking procedure. The effects of carbonization conditions on the microstructure and morphology of HPC were investigated, and the adsorption of methylene blue (MB) on HPC was explored. The as-prepared HPC has a hierarchical micro-, meso- and macropore structure, which results from the overlap of hollow nanospheres possessing microporous shells and macroporous cavities. The carbonization temperature, carbonization time and carbonization heating rate played important roles in tailoring the nanostructures of HPC. The BET specific surface area and micropore specific surface area can reach 2388 m2·g−1 and 1892 m2·g−1, respectively. Benefitting from the well-developed pore structure, the MB removal efficiency can reach 99% under optimized conditions. The adsorption kinetics and thermodynamics can be well described by a pseudo-second-order model and Langmuir model, respectively. Furthermore, such adsorption is characterized by a spontaneous endothermic process.
Effects of Adding Carbon Sources and Nutrient Salts on the Acidification and Microbial Community Structure of Coal Gangue
Authors: Zhu Qi , Ruan Mengying , Hu Zhenqi , Ye Chun
Published: November 2021

Acidic pollution from gangue oxidation has become a primary environmental problem in coal mining areas in China. The use of microorganisms to remediate acidic pollution in coal gangue piles has been indicated to be effective, but environmental differences and carbon sources in different mining areas have become important factors restricting microbial activity. Instead of the addition of new functional bacteria to gangue piles, carbon sources and nutrient salts were added to recently discharged gangue to enhance the activity of beneficial bacteria in the indigenous microbial community. The changes in pH and electrical conductivity (EC) of the gangue leachate as well as the composition and abundance of the functional microbial community on the surface of the gangue were analyzed by leaching simulation experiments and 16S rRNA sequencing. The results showed that (1) the addition of a carbon source maintained the pH of the gangue leachate at 6.31~6.65, which was significantly higher than that of the control group, but the pH of the leachate decreased significantly after the addition of the carbon source was stopped. (2) The addition of carbon sources and nutrient salts changed the microbial community composition on the surface of the gangue, and the species diversity index decreased. The dominant genera in the experimental group were Listeria, Arthrobacter, and Enterococcus; Thiobacillus was not the dominant genus in any of the treatments. (3) The functional gene types in the experimental and control groups were almost the same, but their relative abundance changed. The abundance of functional genes related to the sulfur cycle increased substantially in the experimental group, and the abundance of genes involved in the nitrogen and carbon cycles also increased, albeit to different degrees.
Virological Non-Suppression and Associated Factors Among Adolescents and Youth Living with HIV in Ethiopia: A Facility-Based Case-Control Study
Authors: Dires Yilkal Muchie , Manyazewal Tsegahun , Charlotte Hanlon
Published: November 2021

Background: Virological non-suppression remains the major cause of treatment failure and mortality among adolescents and youth living with HIV in low- and middle-income countries (LMICs). Behavioral and mental health disorders affect adherence to antiretroviral therapy (ART) and the underlying virological suppression, but evidence from LMICs is relatively limited. We aimed to assess HIV virological non-suppression and associated factors among adolescents and youth attending ART in Ethiopia. Methods: A 1:2 matched facility-based case-control study was conducted at ALERT Hospital, Addis Ababa, Ethiopia, among patients with HIVaged 12-24 years who were on ART and with a viral load > (case) or < (control) than 1000 copies/ml after at least six months on ART. The primary outcome was viral load non-suppression: a plasma viral load > 1000 copies/ ml based on two consecutive viral load measurements every three months. The primary exposure was depressive symptoms as measured using the PHQ-9 (Patient Health Questionnaire, 9-item version). Other variables include adherence to ART and sociodemographic characteristics of participants. Data were collected using structured, blinded lay interviewer-administered questionnaires, and data extraction forms. Conditional multivariable logistic regression was conducted using STATA version 14.2 to test the main hypotheses. Results: The study enrolled 192 participants, with a median age of 18 years and 52.1% males. None of the participants reported smoking or chewing Khat (a leaf that stimulates mood). With the PHQ-9 tool, 47%, 28%, and 25% had minimal or no, probable to mild and moderate to severe depression symptoms, respectively. Having depressive symptoms is associated with a higher conditional likelihood of virological failure (odds ratio [OR] = 1.8, p < 0.001) and the association was stronger among those who have moderate or severe depressive symptoms (adjusted OR = 5, 95% CI [1.7-14.6] )and unadjusted OR = 6.8, 95% CI [3.1-14.8]). Having moderate and severe depression is associated with non-adherence to ART (adjusted OR = 10.5). Conclusion: Non-adherence to ART medications, depressive symptoms, advanced WHO stage on ART initiation, poor social support and orphan status were independently associated with virological failure among HIV-infected adolescents and youths on ART. Having moderate and severe depression has higher odds of non-adherence and virological failure. We recommend integration and task-sharing of mental health and HIV care services to reduce virological failure in this group of people.
WITHDRAWN: Understanding of Comorbidities Using Modeling Techniques on EHR
Authors: Badki Meenal
Published: November 2021

Comorbidities refer to the existence of numerous, co-occurring diseases in medicine. The course of one comorbidity is typically extremely dependent on the course of the other condition due to their co-occurrence, and therapies can have major spill-over effects. Despite the high occurrence of comorbidities among patients, there is no complete statistical framework for modelling comorbidity longitudinal dynamics. We propose a probabilistic approach for studying comorbidity dynamics in patients over time in this paper. This approach is a non-homogenous transition technique/mechanism using Hidden Markov Model called as coupled-HMM. Clinical research influenced the design of our coupled-HMM: (1) It accounts for different disease stages (acute, stable) in disease progression by providing clinically meaningful latent phases. (2) It simulates a relationship between the trajectories of comorbidities and the dynamics of capturing co-evolution. (3) The transition mechanism takes into account between-patient heterogeneity (e.g., risk factors, treatments). Based on 675 health trajectories, we assessed our proposed Coupled-HMM, which investigates the concomitant evolution of diabetes mellitus and chronic liver disease. We find that our Coupled-HMM provides a superior fit when compared to competing models without coupling. We also assess the spill-over impact, or the amount to which diabetic therapies are linked to a shift in chronic liver disease from an acute to a stable condition. Immediate application in treatment planning and clinical research becomes possible as a result of our approach in context of comorbidities.
Ovarian Yolk Sac Tumor in a Postmenopausal Patient with Malignant Müllerian Mixed Tumor: A Case Report and Systematic Review of the Literature
Authors: He Shuqian , zhou ping , Cheng Yuxia , Cui Jing , Qi Huangpeng , Sun Qing
Published: November 2021

Background Yolk sac tumor (YST) is a most common form of malignant ovarian germ cell tumors and typically occur in young women and adolescent. Ovarian YST occurred in postmenopausal women is an extremely rare entity which has an aggressive behavior and worse outcome. The aim of this article was to provide better understanding of clinicopathological characteristics, management, and prognosis of postmenopausal YSTs.Case presentation A 64-year-old woman was admitted to our hepatobiliary surgery department due to a cystic and solid round mass in the lower right abdomen in December 2013. laboratory tests showed a-fetoprotein (AFP), carcinoembryonic antigen (CEA), and cancer antigen 19-9 (CA19-9) to be within normal limits, but serum cancer antigen (CA-125) was elevated. Exploratory laparotomy was performed including tumorectomy, right oophorectomy, partial omentectomy. Postoperatively, pathologic findings showed that the morphologic features and immunohistochemical results were consistent with ovarian YST with malignant Müllerian mixed tumor (MMMT). The patient was admitted again for recurrence of the tumor and metastasis to bilateral supraclavicular lymph nodes in August 2018. The patient underwent total abdominal hysterectomy (TAH), left salpingo-oophorectomy, right salpingectomy, pelvic lymphadenectomy, and partial peritonectomy. The patient received 6 cycles of adjuvant chemotherapy with BEP (bleomycin, etoposide, and cisplatin) postoperatively, and died of disease in August 2019. Conclusion Here, we presented a case of postmenopausal YST with MMMT. Meanwhile, a systematic literature review was conducted in the PubMed database, including case reports and case series. We summarized the clinical characteristics, treatments, and prognosis of all cases. Overall survival was calculated with Kaplan-Meier curves. Univariate analysis was performed with the log-rank test. A Cox model was constructed to determine independent predictors of mortality. As the morphological and immunophenotypical features of postmenopausal YSTs were always overlapped with somatic epithelial carcinomas, pathologists should have a high index of suspicion to diagnose ovarian YSTs in older women. Optimum chemotherapy is essential in improving prognosis of postmenopausal YSTs.
(Epidemiological Analysis of Varicella in Dalian from 2009 to 2019 and Application of Three Kinds of Model in Prediction Prevalence of Varicella）Study Design: Cross Sectional Study
Authors: Cheng Tingting , Bai Yu , Sun Xianzhi , Ji Yuchen , Zhang Fan , Li Xiaofeng
Published: November 2021

Objective：This study described the epidemic characteristics of varicella in Dalian from 2009 to 2019, explored the fitting effect of Grey model first-order one variable( GM(1,1)), Markov model, and GM(1,1)-Markov model on varicella data, and found the best fitting method for this type of data, to better predict the incidence trend.Methods: In this study, the epidemiological characteristics of varicella from 2009 to 2019 were analyzed by epidemiological descriptive methods. Using the varicella incidence data from 2009 to 2018, predicted 2019 and compared with actual value. First made GM (1,1) prediction and Markov prediction. Then according to the relative error of the GM(1,1), made GM(1,1)-Markov prediction. Results: This study collected 37223 cases from 2009 to 2019. The average annual incidence was 50.56/100000. Varicella occurred all year round, it had a bimodal distribution. The number of cases had two peaks from April to June and November to January of the following year. The ratio of males to females was 1.167:1. The 4 to 25 accounted for 60.36% of the total population. The age of varicella appeared to shift backward. Students, kindergarten children, scattered children accounted for about 64% of all cases. The GM(1,1) model prediction result of 2019 would be 53.6425, the relative error would be 14.42%, the Markov prediction result would be 56.2075, the relative error would be 10.33%, and the Gray(1,1)-Markov prediction result would be 59.508. The relative error would be 5.06%.Conclusions: Varicella data had its unique development characteristics. The accuracy of GM (1,1) - Markov model is higher than GM(1.1) model and Markov model. The model can be used for prediction and decision guidance.
Thicknesses of Retinal Layers in patients with Graves’ Disease with or Without Orbitopathy
Authors: ogmen berna evranos , Ugurlu Nagihan , Bilginer Muhammet Cuneyt , Polat Sefika Burcak , Genc Birgul , Ersoy Reyhan , Cakir Bekir
Published: November 2021

Purpose: Graves’ ophthalmopathy (GO) is an inflammatory process that may involve the ocular surface, orbital fat, extraocular muscles, and optic nerves in patients with Graves’ disease (GD). We aimed to compare thicknesses of retinal layers in patients with GD with and without GO.Methods: One hundred seven patients with GD (23 with GO (Group1), 84 without GO (Group2)) and eighteen volunteers (Group3) were enrolled. The spectral-domain optical coherence tomography (SD-OCT) is used for ophthalmologic evaluation. Seven retinal layers including retinal nerve ﬁber layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), retinal pigment epithelium (RPE) were assessed. The thicknesses of layers are compared in groups.Results: The median GCL thickness values in groups 1,2, and 3 were 14 µm, 15 µm, and 17.5 µm, respectively (p = 0.02). The median IPL thickness was 20 µm in group 1, 21 µm in group 2, and 22 µm in group 3 (p = 0.038). The median RPE thickness values in groups 1,2, and 3 were 16 µm, 17 µm and 18.5 µm, respectively. The median GCL, IPL, RPE were different in the groups, while RNLF, INL, OPL, ONL were similar (p>0.05 for each). Conclusion: The median GCL, IPL and RPE were thinner in patients with GO than healthy controls.
Two-Sidedness, Flexible, Durable, Highly Transparent and Haze Plastic-Paper For Green Optoelectronics
Authors: Wang Wenliang , Wang Xubiao , Zhao Xingjin , Ren Xiaoxiao , Jiang Weikun , Zhang Zhao
Published: November 2021

We combine biodegradable plastic (polyvinyl alcohol/glycerol polymer), and cellulose-based tissue paper in a very short time (5 s) by a facile way to form a new matrix, named plastic-paper, which has high optical transmittance (~89%) and high optical haze (~90%) in broadband. Results showed that the plastic-paper is durable in different solvents, with high folding strength (over 3500 folding times) and good mechanical strength (＞30 MPa tensile strength). Interestingly, the plastic-paper presents a two-sidedness of surface morphology. The rough side shows high light transmittance and the smooth side presents high optical haze. The smooth side of the plastic-paper is successfully applied to Organic Light-Emitting Diode (OLED). When the rough surface of plastic-paper is used in solar cells, the maximum output power increases by 2.6% compared with bare solar cells. The developed plastic-paper with high optical transmittance and high optical haze holds a great potential in flexible optoelectronic devices.
Effect of Climate Change on the Rainfall Trends: A case study of Pune, India
Authors: Shah Shalaka , Londhe Shreenivas
Published: November 2021

It is the need of the hour to predict the impact of climate change, especially rainfall on the future environmental conditions on local as well as global scales. The present work aims at studying the impact of climate change on the rainfall occurring over Pune, the eighth largest city in India. The General Circulation Models (GCMs) are predominantly used to obtain the climate data all over the globe, at various grid points, for past and future years. Rainfall values obtained from these grid points need to be downscaled to make them location specific. This study proposes a soft computing tool, Artificial Neural Network (ANN) for the purpose of downscaling. The rainfall data at 4 grid points surrounding Pune, was extracted from 5 different GCMs and given as input to ANN with observed rainfall as output, thus forming 5 models. For comparison, a pre-existing downscaling technique, Distribution based scaling (DBS) was used. The coefficient of correlation (r) showed that ANN was working better than DBS. The value of r for ANN was 0.73 for its least accurate model whereas DBS managed to reach 0.73 for its most accurate model. The future rainfall estimated with the help of the trained ANN models show an increase in mean rainfall over the Pune region by ∼2 – 15% and decrease in maximum rainfall by ∼40 – 65%. Peak prediction of rainfall simulated by ANN was not very accurate and hence there is still an opportunity for improvement which is the future scope of this study.
Non-Cognitive Characteristics of Older Adults With Mild Cognitive Impairment And Errors On The Clock Drawing Test
Authors: Umegaki Hiroyuki , Suzuki Yusuke , Komiya Hitoshi , Watanabe Kazuhisa , Nagae Masaki , Yamada Yosuke , Kuzuya Masafumi
Published: November 2021

Individuals with mild cognitive impairment (MCI) often make qualitative errors on the Clock Drawing Test (CDT), and these errors are reported to be associated with lower scores on neuropsychological assessments. However, the association of CDT errors with non-cognitive characteristics has not been investigated in individuals with MCI to date. In this study, we explored this association in older adults with MCI who made qualitative errors on the CDT. Outpatients at a memory clinic with a Clinical Dementia Rating (CDR) of 0.5 (n = 193) were included. The CDT was evaluated using Cahn et al.’s method and non-cognitive characteristics were compared between participants with and without CDT errors. The non-cognitive characteristics compared included normal and maximum gait speeds, grip strength, muscle mass, and severity of white matter lesions. Participants with the CDT error type of conceptual deficit (CD; n = 58) had significantly slower normal and maximum gait speeds, and multiple logistic regression analysis adjusted for age and sex revealed CD was significantly associated with the slowest tertile of maximum gait speed. No other non-cognitive characteristics were significantly associated with errors on the CDT.
Spirometry Has Added Value Over Electrodermal Activity as a Physiological Marker of Mental Load.
Authors: Neukirchen Tobias , Stork Moritz , Hoppe Matthias , Vorstius Christian
Published: November 2021

The objective distinction of different types of mental demands as well as their intensity is relevant for research and practical application but poses a challenge for established physiological methods. We investigated whether respiratory gases (oxygen uptake and carbon dioxide output) are suitable to distinguish between emotional and cognitive demands. To this end, we compared the application of spirometry with an established procedure, namely electrodermal activity (EDA). Our results indicate that electrodermal activity shows a strong responsivity to emotional stress induction, which was highly correlated with its responsivity to cognitive load. Respiratory gases were both sensitive and specific to cognitive load and had the advantage of being predictive for cognitive performance as well as self-reported emotional state. These results support the notion that respiratory gases are a valuable complement to common physiological procedures in the detection and discrimination of different mental demands.
Inter- and Intra-observer Reliability of the “assessment of Motor Repertoire- 3 to 5 Months” Based on Video Recordings of Infants With PWS
Authors: Wang Jun , Shen Xiushu , Yang Hong , Shi Wei , Zhu Xiaoyun , Gao Herong , Yin Huanhuan , Meng Fanzhe , Wu Yun
Published: November 2021

BackgroundThe “Assessment of Motor Repertoire - 3 to 5 Months”, which is part of Prechtl's General Movement Assessment (GMA), has been gradually applied to infants with genetic metabolic disorders. However, there still have been no studies on the application of GMA for infants with Prader-Williams Syndrome (PWS).Aimsthe purpose of this study was to determine inter- and intra-observer reliability of the assessment tool in PWS population.Study designReliability and agreement study.SubjectsThis was a cross-sectional study of 15 infants with PWS born at average gestational age 38 weeks.Outcome measuresStandardized video recordings of 15 infants with PWS (corrected ages 3 to 5 months) were independently assessed by three observers. Kappa and ICC statistics were applied in inter- and intra-observer reliability analysis.ResultsThe overall reliability ICCs values of “Motor Optimality Score” (MOS) ranged from 0.84 to 0.98 and the regarding pairwise agreement ranged between 0.86 and 0.95 in inter- observe reliability. In addition, ICC values for MOS ranged between 0.95 and 0.98 for respectively testers agreement in intra-observer reliability.The complete agreement reliability (100%) was achieved in subcategories of “Fidgety Movements” and “Movement Character” for the inter- and intra-observer. Moderate to high inter- and intra-observer reliability were found in subcategories of “Repertoire of Co-Existent Other Movements”, “Quality of Other Movements” and “Posture”, with kappa values ranging between 0.63 and 1.00. Conclusionhere were high levels of inter-and intra-observer agreement in the “Assessment of Motor Repertoire - 3 to 5 Months” for infants with PWS. It will be possible to carry out standardized quantitative assessment on the motor performance infants with PWS.
The Tumor Microenvironment of Cutaneous Squamous Cell Carcinoma in Immunosuppressed Patients
Authors: Glaun Mica , Gleber-Netto Frederico , Nagarajan Priyadharsini , Xie Tongxin , Covello Jennifer , Akhter Shamima , Adewale Adebayo , Baruch Erez , Wong Michael , Chu Emily , Tsai Kenneth , Flores Elsa , Migden Michael , Silverman Deborah , Goepfert Ryan , Ge Yejing , Sharma Padmanee , Allison James , Myers Jeffrey , Gross Neil , Amit Moran
Published: November 2021

Nonmelanoma skin cancer is the most common malignancy and immunosuppression is a key risk factor. Despite the promising data demonstrating the efficacy of immune checkpoint inhibition in the treatment of cutaneous squamous cell carcinoma (cSCC), most immunosuppressed patients are not included in immunotherapy trials due to the risk for toxicity and the lack of data regarding cSCC immune landscape in immunosuppressed patients. To characterize the specific alterations accounting for a diminished antitumor immune response in immunosuppressed patients, we used multispectral imaging on cSCC pathology specimens from immunosuppressed patients with age and stage matched immunocompetent controls. We show that densities of CD68+ cells are diminished in immunosuppressed patients. Moreover, using an organ transplant recipient cohort from two cancer centers, we found significantly lower effector T-cells densities as compared with controls. Overall, density of CD68+ and CD8+LAG3+ cells were predictors of disease-specific and disease-free survival. These findings provide insight into the patterns of immune infiltrating cells in patients with different types of immunosuppression; leading us to conjecture that different immune based therapeutic approaches may be needed to treat immunosuppressed cSCC patients.
Image-Free Handheld Robot for Total Knee Arthroplasty Improves Early Functional Recovery Compared with Conventional Total Knee Arthroplasty
Authors: Matsumoto Kazu , Ogawa Hiroyasu , Yoshioka Hiroki , Nakamura Yutaka , Akiyama Haruhiko
Published: November 2021

This study aimed to examine whether it is advantageous in robotic-assisted TKA (RA-TKA) compared with conventional TKA throughout a 14 postoperative days (PODs). A total of 113 knees (100 patients) were reviewed and divided into the control group (55 knees) and the RA-TKA group (58 knees). We assessed postoperative pain intensity using a visual analogue scale at rest (rVAS) and during movement (mVAS), evaluated lower extremity functional recovery through quadriceps muscle strength and knee range of motion (ROM), preoperatively and on PODs 3, 7, 10, and 14. We also assessed the rescue analgesia intake and postoperative implant coronal alignment. The mean rVAS and mVAS scores did not differ significantly between the two groups. Muscle strength recovery was significantly faster in the RA-TKA group than in the control group on every PODs. ROM recovery was better in the RA-TKA group than in the control group on POD 10. The amount of postoperative analgesia was significantly lower in the RA-TKA group than in the control group. Attainment of a β angle <2° significantly better in RA-TKA. This study demonstrated better functional recovery in RA-TKA, particularly for muscle strength and ROM. RA-TKA reduced rescue drug intake and provided better implant positioning.
The Accuracy and Effectiveness of Automatic Pedicle Screw Trajectory Planning Based on CT Values: An in Vitro Osteoporosis Model Study.
Authors: Liu JiaBin , Zuo Rui , Zheng WenJie , Li ChangQing , Zhang Chao , Zhou Yue
Published: November 2021

BackgroundPedicle screw placement in patients with osteoporosis is a serious clinical challenge. The bone mineral density (BMD) of the screw trajectory has been positively correlated with the screw pull-out force, while the CT value has been linearly correlated with the BMD. The purpose of this study was to establish an in vitro osteoporosis model and verify the accuracy and effectiveness of automated pedicle screw planning software based on CT values in this model.MethodsTen vertebrae (L1-L5) of normal adult pigs were randomly divided into a decalcification group and a control group. In the decalcification group, the vertebral bodies were decalcified with Ethylenediaminetetraacetic acid (EDTA) to construct an in vitro osteoporosis model. In the decalcification group, automatic planning (AP) and conventional manual planning (MP) were used to plan the pedicle screw trajectory on the left and right sides of the pedicle, respectively, and MP was used on both sides of the control group. CT values of trajectories obtained by the two methods were measured and compared. Then, 3D-printed guide plates were designed to assist pedicle screw placement. A biomechanical instrument was used to measure the pull-out force of the trajectory obtained by the two methods.ResultsAfter decalcification, the T' value of the vertebral body decreased from -0.03 ± 1.03 to -3.03 ± 0.29 (P <0.05). In the decalcification group, the MP trajectory CT value was 2167.28 ± 65.62 Hu, the AP trajectory CT value was 2723.96 ± 165.83 Hu, and the MP trajectory CT value in the control group was 2242.94 ± 25.80 Hu (P<0.05). In the decalcified vertebrae, the screw pull-out force of the MP group was 48.6% lower than that of the control group (P <0.05). The pull-out force of the AP trajectory was 44.7% higher than that of the MP trajectory (P <0.05) and reached 97.4% of the MP trajectory in the control group (P >0.05).ConclusionAutomatic planning of the pedicle screw trajectory based on the CT value can obtain a higher screw pull-out force, which is a valuable new method of pedicle screw placement in osteoporotic vertebral bodies.
Effects of rhBMP-2 Loaded Hydroxyapatite Granules/beta-Tricalcium Phosphate Hydrogel (HA/β-TCP hydrogel) Composite on a Rat Model of Coccygeal Intervertebral Fusion.
Authors: Nakagawa Shinichi , Okada Rintaro , Kushioka Junichi , Kodama Joe , Tsukazaki Hiroyuki , Bal Zeynep , Tateiwa Daisuke , Ukon Yuichiro , Hirai Hiromasa , Makino Takahiro , Takenaka Shota , Okada Seiji , Kaito Takashi
Published: November 2021

The effects and inflammation-related side effects of bone morphogenetic protein (BMP)-2 on posterior lumbar interbody fusion are controversial. One of the potential causes for the inconsistent results is the uncontrolled release of BMP-2 from the collagen carrier. Therefore, BMP delivery systems which support effective bone regeneration while attenuating side effects are strongly sought for. We developed NOVOSIS putty (NP), a novel composite material of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP)-hydrogel, and BMP-2, which can sustainably release BMP-2 over two weeks. This study investigated the effects and side effects of NP compared with those of collagen sponge (CS) containing BMP-2 using a rat coccygeal intervertebral fusion model. The fusion rates of NP with low and high doses of BMP-2 were significantly higher than those of an iliac bone (IB) graft, but those of CS with low and high doses of BMP-2 were not different from those of the IB graft. Furthermore, the incidences of ectopic bone formation and soft tissue swelling were significantly lower in the NP group than in the CS group. The HA/β-TCP hydrogel carrier enabled superior bone induction with low-dose BMP-2 and decreased the incidence of side effects caused by high-dose BMP-2 compared with that of the collagen carrier.
New Putative Long Non-Coding RNAs (lncRNA) Revealed by Pan-Transcriptome of the Emerging Human Pathogenic Fungus Talaromyces Marneffei
Authors: Barbosa David Aciole , Simeone Alexandre Santos , Humberto Ana Carolina , de Maria Yara Natercia Lima Faustino , de Oliveira Regina Costa , Jabes Daniela L. , Nunes Luiz R. , Menegidio Fabiano Bezerra
Published: November 2021

Previous genomic/transcriptomic analyses of Talaromyces marneffei (TM) unravelled relevant pathogenicity-related elements, as well as chromosomal regions potentially involved with the production of non-coding RNAs (ncRNAs), which have been parsimoniously reported in fungi. This manuscript describes a comprehensive pan-transcriptome assembly for TM that identifies a series of previously undetected genetic elements in this emerging pathogenic fungus. Our results confirm that ~58.28% of the 9,480 genes currently annotated in the TM genome are, in fact, transcribed in vivo and that ~23.6% of them may display alternative isomorphs. Moreover, we identified 585 transcripts that do not match any gene currently mapped in the genome, represented by 90 coding transcripts and 140 ncRNAs, including 48 long non-coding RNAs (lncRNAs). Overall, we expect that the novel elements described herein may contribute to improve the currently available Talaromyces databases and foster studies aiming at characterizing lncRNA-mediated gene expression control in fungi.
Genome-Wide Identification of Chicken Bursae of Fabricius miRNAs in Response To Very Virulent Infectious Bursal Disease Virus
Authors: Huang Xuewei , Li Yue , Wang Xiaona , Zhang Junyan , Wang Li , Zhou Han , Jiang Yanping , Cui Wen , Qiao Xinyuan , Li Yijing , Tang Lijie
Published: November 2021

Infectious bursal disease virus (IBDV) can cause a highly contagious immunosuppressive disease in young chickens, resulting in considerable economic losses to the poultry industry. MicroRNAs (miRNAs) are crucial regulators of gene expression and are involved in the pathogenesis of IBDV infection. To investigate the roles of miRNA in chicken bursae of Fabricius in response to very virulent IBDV (vvIBDV) infection, RNA sequencing was performed. For this, we established an IBDV infection model as observed using histopathology, transmission electron microscopy (TEM), viral load detection, and cytokine expression levels. In total, 77 differentially expressed (DE) miRNAs were identified, of which 42 were upregulated and 35 were downregulated. A gene ontology analysis showed that genes associated with cellular processes, cells, and binding were enriched, and pathway analyses suggested that axon guidance, tight junctions, and endocytosis may be activated following vvIBDV infection. Moreover, we predicted the target genes of DE miRNAs and constructed an miRNA-mRNA regulatory network. In total, 189 pairs of miRNA-target genes were identified, comprising 67 DE miRNAs and 73 mRNAs. In this network, gga-miR-1684b-3p was identified with the highest fold change, and gga-miR-1788-3p and gga-miR-3530-5p showed a high degree of change, suggesting that they play vital roles in vvIBDV-host interactions. This study is the first to perform a comprehensive analysis of DE miRNAs in the bursa of Fabricius in response to vvIBDV infection, and it provides new insights into the molecular mechanisms underlying vvIBDV infection and pathogenesis.