bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Variation in CNS Myelination and Functional Brain Connectivity in Recombinant Inbred Mice
Authors: Goudriaan Andrea , Loos Maarten , Spijker Sabine , Smit August B. , Verheijen Mark H. G.
Published: September 2020

Myelination greatly increases the speed of action potential propagation of neurons, thereby enhancing the efficacy of inter-neuronal communication and hence, potentially, optimizing the brain’s signal processing capability. The impact of genetic variation on the extent of axonal myelination and its consequences for brain functioning remain to be determined. Here we investigated this question using a genetic reference panel (GRP) of mouse BXD recombinant inbred (RI) strains, which partly model genetic diversity as observed in human populations, and which show substantial genetic differences in a variety of behaviors, including learning, memory and anxiety. We found coherent differences in the expression of myelin genes in brain tissue of RI strains of the BXD panel, with the largest differences in the hippocampus. The parental C57BL/6J (C57) and DBA/2J (DBA) strains were on opposite ends of the expression spectrum, with C57 showing higher myelin transcript expression compared with DBA. Our experiments showed accompanying differences between C57 and DBA in myelin protein composition, total myelin content, and white matter conduction velocity. Finally, the hippocampal myelin gene expression of the BXD strains correlated significantly with behavioral traits involving anxiety and/or activity. Taken together, our data indicate that genetic variation in myelin gene expression translates to differences observed in myelination, axonal conduction speed, and possibly in anxiety/activity related behaviors.
Therapeutic Benefit of the Association of Lodenafil with Mesenchymal Stem Cells on Hypoxia-induced Pulmonary Hypertension in Rats
Authors: Silva Marina de Moraes Carvalho da , Alencar Allan Kardec Nogueira de , Silva Jaqueline Soares da , Montagnoli Tadeu Lima , Silva Grazielle Fernandes da , Rocha Bruna de Souza , Montes Guilherme Carneiro , Mendez-Otero Rosália , Pimentel-Coelho Pedro Moreno , Vasques Juliana F. , Trahez Margarete Manhães , Sudo Roberto Takashi , Zapata-Sudo Gisele
Published: September 2020

Pulmonary arterial hypertension (PAH) is characterized by the remodeling of pulmonary arteries, with an increased pulmonary arterial pressure and right ventricle (RV) overload. This work investigated the benefit of the association of human umbilical cord mesenchymal stem cells (hMSCs) with lodenafil, a phosphodiesterase-5 inhibitor, in an animal model of PAH. Male Wistar rats were exposed to hypoxia (10% O2) for three weeks plus a weekly i.p. injection of a vascular endothelial growth factor receptor inhibitor (SU5416, 20 mg/kg, SuHx). After confirmation of PAH, animals received intravenous injection of 5.105 hMSCs or vehicle, followed by oral treatment with lodenafil carbonate (10 mg/kg/day) for 14 days. The ratio between pulmonary artery acceleration time and RV ejection time reduced from 0.42 ± 0.01 (control) to 0.24 ± 0.01 in the SuHx group, which was not altered by lodenafil alone but was recovered to 0.31 ± 0.01 when administered in association with hMSCs. RV afterload was confirmed in the SuHx group with an increased RV systolic pressure (mmHg) of 52.1 ± 8.8 normalized to 29.6 ± 2.2 after treatment with the association. Treatment with hMSCs + lodenafil reversed RV hypertrophy, fibrosis and interstitial cell infiltration in the SuHx group. Combined therapy of lodenafil and hMSCs may be a strategy for PAH treatment.
Regulation of Apoptosis by the Bcl-2 Family of Proteins: Field on a Brink
Authors: Ladokhin Alexey S.
Published: September 2020

Apoptosis, a form of programmed cell death, is a highly regulated process critical for tissue development, homeostasis, and pathogenesis of various diseases [...]
Elementary Classroom Teachers’ Self-Reported Use of Movement Integration Products and Perceived Facilitators and Barriers Related to Product Use
Authors: Dugger Roddrick , Rafferty Aaron , Hunt Ethan , Beets Michael , Webster Collin , Chen Brian , Rehling Jeff , Weaver Robert Glenn
Published: September 2020

Movement integration (MI) products are designed to provide children with physical activity during general education classroom time. The purpose of this study was to examine elementary classroom teachers’ self-reported use of MI products and subsequent perceptions of the facilitators of and barriers to MI product use. This study utilized a mixed-methods design. Elementary classroom teachers (n = 40) at four schools each tested four of six common MI products in their classroom for one week. Teachers completed a daily diary, documenting duration and frequency of product use. Following each product test, focus groups were conducted with teachers to assess facilitators and barriers. MI product use lasted for 11.2 (Standard Deviation (SD) = 7.5) min/occasion and MI products were used 4.1 (SD = 3.5) times/week on average. Activity Bursts in the Classroom for Fitness, GoNoodle, and Physical Activity Across the Curriculum were most frequently used. Facilitators of and barriers to MI product use were identified within three central areas—logistics, alignment with teaching goals, and student needs and interests. Teachers were receptive to MI products and used them frequently throughout the week. When considering the adoption of MI products, teachers, administrators, and policy makers should consider products that are readily usable, align with teaching goals, and are consistent with student needs and interests.
The Parent’s Chronotype and Child’s Sleeping Quality in Association with Relationship Satisfaction
Authors: Ricci Cristian , Parra-Robledo Zaida , Rothenbacher Dietrich , Díaz-Morales Juan Francisco , Genuneit Jon
Published: September 2020

The prospective Ulm-SPATZ study was investigated to assess the role of child sleeping quality between 4 to 6 years of age in affecting a partner’s sleeping and relationship satisfaction within a couple. The study was conducted using a triadic approach in which the child was included in the Actor-Partner-Interdependence Model (APIM). Sleeping quality of the child was determined by using the German version of the children’s sleep habits questionnaire, sleeping features of the parents were assessed by using the Munich chronotype questionnaire, and the partner relationship assessment was performed by employing the German version of the parenting stress index questionnaire. In 211 German triads, we observed that sleeping characteristics and partner relationship scores at different child ages are consistent for both men and women. Higher and statistically significant sleep duration, time spent in bed, the midpoint of sleep, time getting out of bed, and sleep onset in women compared to men during the working days were observed. The APIM analyses showed a significant direct effect of child sleep quality on the partner relationship satisfaction. In women, a mediated effect of child sleep quality acted through sleep duration and time spent in bed on the partner relationship satisfaction score during both free and working days. In men, low child sleep quality was found to be associated with increased sleep onset during both free and working days. Child sleep quality influences relationship satisfaction mostly in mothers, likely because of their higher involvement in childcare during working days. Distress in the couple could be counteracted by a major involvement of the fathers in child management.
Research Progress of Graphene-Based Materials on Flexible Supercapacitors
Authors: Du Yongquan , Xiao Peng , Yuan Jian , Chen Jianwen
Published: September 2020

With the development of wearable and flexible electronic devices, there is an increasing demand for new types of flexible energy storage power supplies. The flexible supercapacitor has the advantages of fast charging and discharging, high power density, long cycle life, good flexibility, and bendability. Therefore, it exhibits great potential for use in flexible electronics. In flexible supercapacitors, graphene materials are often used as electrode materials due to the advantages of their high specific surface area, high conductivity, good mechanical properties, etc. In this review, the classification of flexible electrodes and some common flexible substrates are firstly summarized. Secondly, we introduced the advantages and disadvantages of five graphene-based materials used in flexible supercapacitors, including graphene quantum dots (GQDs), graphene fibers (GFbs), graphene films (GFs), graphene hydrogels (GHs), and graphene aerogels (GAs). Then, we summarized the latest developments in the application of five graphene-based materials for flexible electrodes. Finally, the defects and outlooks of GQDs, GFbs, GFs, GHs, and GAs used in flexible electrodes are given.
Thermal Stability of Plasma-Sprayed Thick Thermal Barrier Coatings Using Triplex ProTM-200 Torch
Authors: Tao Shiqian , Yang Jiasheng , Li Wei , Shao Fang , Zhong Xinghua , Zhao Huayu , Zhuang Yin , Ni Jinxing , Tao Shunyan , Yang Kai
Published: September 2020

Segmentation-crack structured yttria-stabilized zirconia (YSZ) thermal barrier coatings (TBCs) were deposited by atmospheric plasma spraying (APS) using a Triplex Pro™-200 gun. In this work, free-standing coating specimens (~700 μm) were isothermally heat-treated in air from 1200 to 1600 °C for 24 h and at 1550 °C for 20 to 100 h, respectively. The thermal aging behaviors such as microstructures, phase compositions, grain growth and mechanical properties were characterized via scanning electron microscopy (SEM), X-ray diffraction (XRD), electron backscatter diffraction (EBSD) and a Vickers hardness test. The results indicated that the as-sprayed coatings mainly consisted of metastable tetragonal (t′-YSZ) phase, but the t′-YSZ gradually partitioned into equilibrium tetragonal (t-YSZ) and cubic (c-YSZ) phases due to yttrium diffusion during thermal exposure, and with an improvement in temperature, the c-YSZ may retain or transform into another yttrium-rich tetragonal (t″-YSZ) phase. The transformation of t-YSZ to monoclinic phase (m-YSZ) has occurred after 1550 °C/40 h heat treatment, and the content of the m-YSZ phase increased with the prolongation of the thermal exposure time. The variations of Vickers hardness have a correlation with pores healing and grain growth, which might be attributed to the coating sintering and m-YSZ phase formation. Furthermore, the growth pattern of the grains was investigated in detail. In service, cracks and pores proceeded along the grain boundaries, especially surrounding the small grains. It is conducive to the engineering application of TBCs fabricated with the Triplex Pro™-200 gun.
Microwave-Assisted Aldol Condensation of Furfural and Acetone over Mg–Al Hydrotalcite-Based Catalysts
Authors: Tampieri Alberto , Lilic Matea , Constantí Magda , Medina Francesc
Published: September 2020

The depletion of fossil fuel resources has prompted the scientific community to find renewable alternatives for the production of energy and chemicals. The products of the aldol condensation between bio-based furfural and acetone have been individuated as promising intermediates for the preparation of biofuels and polymeric materials. We developed a protocol for the microwave-assisted condensation of these two compounds over hydrotalcite-based materials. Mg:Al 2:1 hydrotalcite was prepared by co-precipitation; the obtained solid was calcined to afford the corresponding mixed metal oxide, which was then rehydrated to obtain a meixnerite-type material. The prepared solids were characterized by PXRD, ICP-AES, TGA-DSC and N2 physisorption, and tested as catalysts in the aldol condensation of acetone and furfural in a microwave reactor. The performance of the catalysts was assessed and compared; the meixnerite catalyst proved to be the most active, followed by the mixed metal oxide and the as-synthesized hydrotalcite, which has often been reported to be inactive. In all cases, the reaction is quite fast and selective, which makes our protocol useful for rapidly converting furfural and acetone into their condensation products.
99mTc-MAG3 Diuretic Renography: Intra- and Inter-Observer Repeatability in the Assessment of Renal Function
Authors: Sachpekidis Christos , Schepers Robin , Marti Monika , Kopp-Schneider Annette , Alberts Ian , Keramida Georgia , Afshar-Oromieh Ali , Rominger Axel
Published: September 2020

The aim of the present study is to evaluate the intra- and inter-observer agreement in assessing the renal function by means of 99mTc-MAG3 diuretic renography. One hundred and twenty adults were enrolled in the study. One experienced and one junior radiographer processed the renograms twice by assigning manual and semi-automated regions of interest. The differential renal function (DRF, %), time to maximum counts for the right and left kidney (TmaxR-TmaxL, min) and time to half-peak counts (T1/2, min) were calculated. The Bland–Altman analysis (bias±95% limits of agreement), Lin’s concordance correlation coefficient and weighted Fleiss’ kappa coefficient were used to assess agreement. Based on the Bland–Altman analysis, the intra-observer repeatability results for the experienced radiographer using the manual and the semi-automated techniques were 0.2 ± 2.6% and 0.3 ± 6.4% (DRF), respectively, −0.01 ± 0.24 and 0.00 ± 0.34 (TmaxR), respectively, and 0.00 ± 0.26 and 0.00 ± 0.33 (TmaxL), respectively. For the junior radiographer, the respective results were 0.5 ± 5.0% and 0.8 ± 9.4% (DRF), 0.00 ± 0.44 and 0.01 ± 0.28 (TmaxR), and 0.01 ± 0.28 and −0.02 ± 0.44 (TmaxL). The inter-observer repeatability for the manual method was 0.6 ± 5.0% (DRF), −0.10 ± 0.42 (TmaxR) and −0.05 ± 0.38 (TmaxL), and for the semi-automated method −0.2 ± 9.1% (DRF), 0.00 ± 0.31 (TmaxR) and −0.05 ± 0.40 (TmaxL). The weighted Fleiss’ kappa coefficient for the T1/2 assessments ranged between 0.85–0.97 for both intra- and inter-observer repeatability with both methods. These findings suggest a very good repeatability in DRF assessment with the manual method—especially for the experienced observer—but a less good repeatability with the semi-automated approach. The calculation of Tmax was also operator-dependent. We conclude that reader experience is important in the calculation of renal parameters. We therefore encourage reader training in renal scintigraphy. Moreover, the manual tool seems to perform better than the semi-automated tool. Thus, we encourage cautious use of automated tools and adjunct validation by manual methods where possible.
Characteristics and Utility of Fluorescein Breakup Patterns among Dry Eyes in Clinic-Based Settings
Authors: Shigeyasu Chika , Yamada Masakazu , Yokoi Norihiko , Kawashima Motoko , Suwaki Kazuhisa , Uchino Miki , Hiratsuka Yoshimune , Tsubota Kazuo ,   on behalf of the DECS-J Study Group
Published: September 2020

(1) Background: To evaluate the characteristics of fluorescein breakup patterns (FBUPs) among patients with dry eye disease (DED) and efficacy of FBUPs as a diagnostic test for DED subgroups. (2) Methods: The study enrolled 449 patients with DED. FBUPs were categorized as follows: area break (AB), line break (LB), spot break (SB), dimple break (DB), and random break (RB). Schirmer value, fluorescein breakup time (FBUT), keratoconjunctival score, DED subgroups and subjective symptoms were examined. (3) Results: LB patients presented with short FBUT and high keratoconjunctival score. AB patients presented with short FBUT, high cornea and keratoconjunctival scores. SB patients were young with short FBUT. DB patients exhibited low keratoconjunctival score. RB patients were young, with long FBUT and low keratoconjunctival scores. Among DED subgroups, LB and AB constituted 74.7% of aqueous-deficiency dry eye (ADDE). SB and DB constituted 42.4% of short FBUT dry eye (short FBUT-DE). Post-test probabilities and positive likelihood ratios for ADDE were 58.7% and 1.63, respectively; those for short FBUT-DE were 46.3% and 2.02, respectively. Patients with SB and AB exhibited significantly severe subjective symptoms than other FBUPs. (4) Conclusions: FBUPs are associated with both objective findings and symptoms of DED and may be a clinical tool for identification of DED subgroups.
CAPTCHA Recognition Using Deep Learning with Attached Binary Images
Authors: Thobhani Alaa , Gao Mingsheng , Hawbani Ammar , Ali Safwan Taher Mohammed , Abdussalam Amr
Published: September 2020

Websites can increase their security and prevent harmful Internet attacks by providing CAPTCHA verification for determining whether end-user is a human or a robot. Text-based CAPTCHA is the most common and designed to be easily recognized by humans and difficult to identify by machines or robots. However, with the dramatic advancements in deep learning, it becomes much easier to build convolutional neural network (CNN) models that can efficiently recognize text-based CAPTCHAs. In this study, we introduce an efficient CNN model that uses attached binary images to recognize CAPTCHAs. By making a specific number of copies of the input CAPTCHA image equal to the number of characters in that input CAPTCHA image and attaching distinct binary images to each copy, we build a new CNN model that can recognize CAPTCHAs effectively. The model has a simple structure and small storage size and does not require the segmentation of CAPTCHAs into individual characters. After training and testing the proposed CAPTCHA recognition CNN model, the achieved experimental results reveal the strength of the model in CAPTCHA character recognition.
BAT—Block Analytics Tool Integrated with Blockchain Based IoT Platform
Authors: Edussuriya Chathurangi , Vithanage Kasun , Bandara Namila , Alawatugoda Janaka , Sandirigama Manjula , Jayasinghe Upul , Shone Nathan , Lee Gyu Myoung
Published: September 2020

The Internet of Things (IoT) is the novel paradigm of connectivity and the driving force behind state-of-the-art applications and services. However, the exponential growth of the number of IoT devices and services, their distributed nature, and scarcity of resources has increased the number of security and privacy concerns ranging from the risks of unauthorized data alterations to the potential discrimination enabled by data analytics over sensitive information. Thus, a blockchain based IoT-platform is introduced to address these issues. Built upon the tamper-proof architecture, the proposed access management mechanisms ensure the authenticity and integrity of data. Moreover, a novel approach called Block Analytics Tool (BAT), integrated with the platform is proposed to analyze and make predictions on data stored on the blockchain. BAT enables the data-analysis applications to be developed using the data stored in the platform in an optimized manner acting as an interface to off-chain processing. A pharmaceutical supply chain is used as the use case scenario to show the functionality of the proposed platform. Furthermore, a model to forecast the demand of the pharmaceutical drugs is investigated using a real-world data set to demonstrate the functionality of BAT. Finally, the performance of BAT integrated with the platform is evaluated.
A Neural Network Decomposition Algorithm for Mapping on Crossbar-Based Computing Systems
Authors: Kim Choongmin , A. Abraham Jacob , Kang Woochul , Chung Jaeyong
Published: September 2020

Crossbar-based neuromorphic computing to accelerate neural networks is a popular alternative to conventional von Neumann computing systems. It is also referred as processing-in-memory and in-situ analog computing. The crossbars have a fixed number of synapses per neuron and it is necessary to decompose neurons to map networks onto the crossbars. This paper proposes the k-spare decomposition algorithm that can trade off the predictive performance against the neuron usage during the mapping. The proposed algorithm performs a two-level hierarchical decomposition. In the first global decomposition, it decomposes the neural network such that each crossbar has k spare neurons. These neurons are used to improve the accuracy of the partially mapped network in the subsequent local decomposition. Our experimental results using modern convolutional neural networks show that the proposed method can improve the accuracy substantially within about 10% extra neurons.
Efficient Approximate Adders for FPGA-Based Data-Paths
Authors: Perri Stefania , Spagnolo Fanny , Frustaci Fabio , Corsonello Pasquale
Published: September 2020

Approximate computing represents a powerful technique to reduce energy consumption and computational delay in error-resilient applications, such as multimedia processing, machine learning, and many others. In these contexts, designing efficient digital data-paths is a crucial concern. For this reason, the addition operation has received a great deal of attention. However, most of the approximate adders proposed in the literature are oriented to Application Specific Integrated Circuits (ASICs), and their deployment on different devices, such as Field Programmable Gate Arrays (FPGAs), appears to be unfeasible (or at least ineffective). This paper presents a novel approximate addition technique thought to efficiently exploit the configurable resources available within an FPGA device. The proposed approximation strategy sums the k least significant bits two-by-two by using 4-input Look-up-Tables (LUTs), each performing a precise 2-bit addition with the zeroed carry-in. In comparison with several FPGA-based approximate adders in the existing literature, the novel adder achieves markedly improved error characteristics without compromising either the power consumption or the delay. As an example, when implemented within the Artix-7 xc7a100tcsg324-3 chip, the 32-bit adder designed as proposed here with k = 8 performs as fast as its competitors and reduces the Mean Error Distance (MED) by up to 72% over the state-of-the-art approximate adders, with an energy penalty of just 8% in the worst scenario. The integration of the new approximate adder within a more complex application, such as the 2D digital image filtering, has shown even better results. In such a case, the MED is reduced by up to 97% with respect to the FPGA-based counterparts proposed in the literature.
Compact Dual-Band Rectenna Based on Dual-Mode Metal-Rimmed Antenna
Authors: Vu Ngoc Anh Ha , Thien Nguyen Minh , Trinh Le Huy , Nguyen Vu Truong , Ferrero Fabien
Published: September 2020

This paper proposes the design of a dual-band integrated rectenna. The rectenna has compact size of 0.4 × 0.3 × 0.25 cm3 and operates at 925 MHz and 2450 MHz bands. In general, the rectenna consists of two main parts, the metal-rimmed dual-band antenna used for harvesting the radio frequency (RF) signals from the environment and the rectifier circuit to convert these receiving powers to the direct current (DC). Because of the dual resonant structure of the antenna, the rectifier circuit can be optimized in terms of size and the frequency bandwidth, while the conversion efficiencies are always obtained 60% at the RF input power −2.5 dBm and −1 dBm for the lower band and the higher band, respectively. Measured results show that the metal-rimmed antenna exhibits −10 dB reflection coefficient in both desired frequency bands. Moreover, the antenna achieves 47% and 89% of total efficiency respectively at 925 MHz and 2450 MHz, which confirms that the proposed rectenna is well applicable in most of the miniaturized wireless sensor networks and IoT systems.
A Cointegration Analysis of the Nordic Roundwood Markets
Authors: Eriksson Victoria , Lundmark Robert
Published: September 2020

The integration of the Nordic timber markets has been analysed to provide market information to various decision-makers, e.g., climate and industrial policies and investment decisions. This study addresses the interlinkage between Nordic (Sweden, Norway and Finland) roundwood markets (Scots pine (Pinus sylvestris L.)) and Norway spruce ((Picea abies L.) sawlogs and pulpwood). In total, eleven markets were analysed using quarterly data over the period 2006Q1–2017Q4 where various unit root and stationary tests were performed together with Johansen’s cointegration test. In addition, directional causality analyses between the integrated markets were also performed. The results show that the law-of-one-price (LOP) hypothesis can be rejected for most of the studied markets and that no individual market emerges as the price-leader. Only the Swedish and Norwegian pine sawlog markets are integrated suggesting a single market for both countries. Price affecting national and forest-related policies as well as investments in the forestry sector, forest industries, bioenergy sector and other forest product using sectors, will not disperse into a larger international market structure; instead, the price effect will be national and more likely have a more profound effect.
Quality Attributes of Cryoconcentrated Calafate (Berberis microphylla) Juice during Refrigerated Storage
Authors: Orellana-Palma Patricio , Tobar-Bolaños Guisella , Casas-Forero Nidia , Zúñiga Rommy N. , Petzold Guillermo
Published: September 2020

This study aimed to evaluate the potential of centrifugal block cryoconcentration (CBCC) at three cycles applied to fresh calafate juice. The fresh juice and cryoconcentrate at each cycle were stored for five weeks at 4 °C and quality attributes were analyzed every 7 days. CBCC had significant effects in the calafate juice, since in the last cycle, the cryoconcentrate reached a high value of total soluble solids (TSS, ≈42 °Brix), with final attractive color, and an increase of approximately 2.5, 5.2, 5.1, 4.0 and 5.3 times in relation to the fresh juice values, for total bioactive compounds (TBC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC), respectively. However, at 35 days under storage, these values decreased by 5%, 13%, 15%, 19%, 24% and 27%, for TSS, TBC, DPPH, ABTS, FRAP and ORAC, respectively. Additionally, until the day 14, the panelists indicated a good acceptability of the reconstituted cryoconcentrate. Therefore, CBCC can be considered a novel and viable technology for the preservation of quality attributes from fresh calafate juice with interesting food applications of the cryoconcentrates due to their high stability during storage time in comparison to the fresh juice.
A Miniaturized QuEChERS Method Combined with Ultrahigh Liquid Chromatography Coupled to Tandem Mass Spectrometry for the Analysis of Pyrrolizidine Alkaloids in Oregano Samples
Authors: Izcara Sergio , Casado Natalia , Morante-Zarcero Sonia , Sierra Isabel
Published: September 2020

Recent and unexpected food alerts about relatively high amounts of pyrrolizidine alkaloids in oregano samples have stressed the need to develop analytical strategies to ensure food safety in this type of foodstuff. Accordingly, this work presents the development of a miniaturized strategy based on the QuEChERS (quick, easy, cheap, effective, rugged and safe) method combined with ultrahigh liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) for the determination of 21 pyrrolizidine alkaloids suggested by the European Food Safety Authority to be monitored in food. The analytical method was properly validated, with overall average recoveries from 77 to 96% and relative standard deviations <13% (n = 9). The method proved to be a sustainable analytical strategy which meets green analytical chemistry principles as it showed good performance by using small amounts of sample (0.2 g), organic solvents (1000 µL), clean-up sorbents (175 mg) and partitioning salts (0.65 g). Its feasibility was verified through the analysis of 23 oregano samples. Of the samples analyzed, 100% were contaminated, with an average concentration of 1254 µg/kg. Lasiocarpine, lasiocarpine N-oxide, europine, europine N-oxide, senecivernine, senecionine, echimidine N-oxide, lycopsamine N-oxide and intermedine N-oxide were the alkaloids which significantly contributed to the contamination of the samples.
Distribution of Antibiotic Resistance Genes in the Saliva of Healthy Omnivores, Ovo-Lacto-Vegetarians, and Vegans
Authors: Milanović Vesna , Aquilanti Lucia , Tavoletti Stefano , Garofalo Cristiana , Osimani Andrea , De Filippis Francesca , Ercolini Danilo , Ferrocino Ilario , Cagno Raffaella Di , Turroni Silvia , Lazzi Camilla , Pellegrini Nicoletta , Clementi Francesca
Published: September 2020

Food consumption allows the entrance of bacteria and their antibiotic resistance (AR) genes into the human oral cavity. To date, very few studies have examined the influence of diet on the composition of the salivary microbiota, and even fewer investigations have specifically aimed to assess the impact of different long-term diets on the salivary resistome. In this study, the saliva of 144 healthy omnivores, ovo-lacto-vegetarians, and vegans were screened by nested PCR for the occurrence of 12 genes conferring resistance to tetracyclines, macrolide-lincosamide-streptogramin B, vancomycin, and β-lactams. The tet(W), tet(M), and erm(B) genes occurred with the highest frequencies. Overall, no effect of diet on AR gene distribution was seen. Some differences emerged at the recruiting site level, such as the higher frequency of erm(C) in the saliva of the ovo-lacto-vegetarians and omnivores from Bologna and Turin, respectively, and the higher occurrence of tet(K) in the saliva of the omnivores from Bologna. A correlation of the intake of milk and cheese with the abundance of tet(K) and erm(C) genes was seen. Finally, when the occurrence of the 12 AR genes was evaluated along with geographical location, age, and sex as sources of variability, high similarity among the 144 volunteers was seen.
Blues in the Brain and Beyond: Molecular Bases of Major Depressive Disorder and Relative Pharmacological and Non-Pharmacological Treatments
Authors: Maffioletti Elisabetta , Minelli Alessandra , Tardito Daniela , Gennarelli Massimo
Published: September 2020

Despite the extensive research conducted in recent decades, the molecular mechanisms underlying major depressive disorder (MDD) and relative evidence-based treatments remain unclear. Various hypotheses have been successively proposed, involving different biological systems. This narrative review aims to critically illustrate the main pathogenic hypotheses of MDD, ranging from the historical ones based on the monoaminergic and neurotrophic theories, through the subsequent neurodevelopmental, glutamatergic, GABAergic, inflammatory/immune and endocrine explanations, until the most recent evidence postulating a role for fatty acids and the gut microbiota. Moreover, the molecular effects of established both pharmacological and non-pharmacological approaches for MDD are also reviewed. Overall, the existing literature indicates that the molecular mechanisms described in the context of these different hypotheses, rather than representing alternative ones to each other, are likely to contribute together, often with reciprocal interactions, to the development of MDD and to the effectiveness of treatments, and points at the need for further research efforts in this field.
Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
Authors: Małodobra-Mazur Małgorzata , Cierzniak Aneta , Pawełka Dorota , Kaliszewski Krzysztof , Rudnicki Jerzy , Dobosz Tadeusz
Published: September 2020

Obesity is a major health problem in highly industrialized countries. High-fat diet (HFD) is one of the most common causes of obesity and obesity-related disorders. There are considerable differences between fat depots and the corresponding risks of metabolic disorders. We investigated the various effects of an excess of fatty acids (palmitic 16:0, stearic 18:0, and oleic acids 18:1n−9) on adipogenesis of subcutaneous- and visceral-derived mesenchymal stem cells (MSCs) and phenotypes of mature adipocytes. MSCs of white adipose tissue were acquired from adipose tissue biopsies obtained from subcutaneous and visceral fat depots from patients undergoing abdominal surgery. The MSCs were extracted and differentiated in vitro with the addition of fatty acids. Oleic acid stimulated adipogenesis, resulting in higher lipid content and larger adipocytes. Furthermore, oleic acid stimulated adipogenesis by increasing the expression of CCAAT enhancer binding protein β (CEBPB) and peroxisome proliferator activated receptor γ (PPARG). All of the examined fatty acids attenuated the insulin-signaling pathway and radically reduced glucose uptake following insulin stimulation. Visceral adipose tissue was shown to be more prone to generate inflammatory stages. The subcutaneous adipose tissue secreted a greater quantity of adipokines. To summarize, oleic acid showed the strongest effect on adipogenesis. Furthermore, all of the examined fatty acids attenuated insulin signaling and secretion of cytokines and adipokines.
The Impact of Chronic Mild Stress and Agomelatine Treatment on the Expression Level and Methylation Status of Genes Involved in Tryptophan Catabolic Pathway in PBMCs and Brain Structures
Authors: Wigner Paulina , Synowiec Ewelina , Jóźwiak Paweł , Czarny Piotr , Białek Katarzyna , Bijak Michal , Szemraj Janusz , Gruca Piotr , Papp Mariusz , Sliwinski Tomasz
Published: September 2020

Depression is the serious mental disorder. Previous studies suggest that the development mechanism of depression may be associated with disorders of the tryptophan catabolic pathway (TRYCAT). Thus, this study investigates the effect of agomelatine treatment on the expression and methylation status of genes involved in TRYCAT in the brain and blood of rats exposed to a chronic mild stress (CMS). Separate groups of rats were exposed to CMS for two or seven weeks; the second group received vehicle or agomelatine for five weeks. After completion of both stress conditions and treatment, the expression levels of messenger RNA (mRNA) and protein, as well as the methylation status of promoters, were measured in peripheral blood mononuclear cells (PBMCs) and in brain structures with the use of TaqMan Gene Expression Assay, Western blot, and methylation-sensitive high-resolution melting techniques. In PBMCs, Kmo mRNA expression increased in the group after CMS, while this effect was normalized by agomelatine therapy. In brain, KatI and KatII expression changed following CMS exposure. Moreover, CMS decreased the methylation status of the second Tdo2 promoter in the amygdala. Protein expression of Tph1, Tph2, Ido1, and KatII changed in the group after CMS and agomelatine administration, most prominently in the basal ganglia, cerebral cortex, hippocampus, and amygdala. The results indicate that CMS and agomelatine affect the mRNA and protein expression, as well as the methylation of promoters of genes involved in the tryptophan catabolic pathway.
Acceptance of Illness and Compliance with Therapeutic Recommendations in Patients with Hypertension
Authors: Pluta Agnieszka , Sulikowska Beata , Manitius Jacek , Posieczek Zuzanna , Marzec Alicja , Morisky Donald E.
Published: September 2020

Arterial hypertension (AH) is one of the most common cardiovascular diseases increasing mortality rates in Poland and worldwide. Due to its prevalence, complications and treatment costs, AH is a significant health-related, economic and social problem. The aim of this study was to assess the level of acceptance of illness and compliance with therapeutic recommendations in patients with AH. The study included 200 outpatient hypertensive patients, 85 men and 115 women aged 49.1 ± 11.6, and used the standardized acceptance of illness (AIS), the eight-item Morisky Medication Adherence Scale (MMAS-8) and author’s design questionnaires. The level of acceptance of illness was found to be as follows: higher in men than in women, unaffected by comorbidities or sociodemographic factors such as residence and professional activity, decreasing with age, and correlating negatively with the duration of antihypertensive therapy. The level of adherence and compliance did not affect the AIS score and increased with the level of education. The study population demonstrated an overall good level of acceptance of illness. Men were characterized by lower levels of adherence and compliance. Patients with AH presented a moderate level of adherence and compliance, which indicates the need for providing active education, support and extensive cooperation facilitating their conformity to therapeutic recommendations.
Decision-Making for Project Delivery System with Related-Indicators Based on Pythagorean Fuzzy Weighted Muirhead Mean Operator
Authors: Cao Yongchao , Li Huimin , Su Limin
Published: September 2020

An appropriate project delivery system plays an essential role in sustainable construction project management. Due to the complexity of practical problems and the ambiguity of human thinking, selecting an appropriate project delivery system (PDS) is an enormous challenge for owners. This paper aims to develop a PDS selection method to deal with the related-indicators case by combining the advantages of Pythagorean fuzzy sets (PFSs) and Pythagorean fuzzy weighted Muirhead mean (PFWMM) operators. The contributions of this paper are as follows: (1) This study innovatively introduced the PFWMM operator to deal with PDS selection problems for the case of the relevance among all indicators affecting PDSs selection in a complex environment. (2) A new method of solving indicators’ weights was proposed to adapt to the related-indicators PDS selection problem, through investigating the differences between the ideal PDS and the alternative PDS under all indicators. (3) A decision-making framework for PDS selection was constructed by comprehensive use of the advantages of PFSs and the PFWMM operator in dealing with related-indicators PDS decision-making problems. An example of selecting a PDS is exhibited to illustrate the effectiveness and applicability of the proposed method.
Influences of Exposure Time and Mortality Assessment Interval on Bioassay Results of Insecticide-Resistant Tropical Bed Bugs (Hemiptera: Cimicidae)
Authors: Leong Xin-Yeng , Singham G. Veera , Shu-Chien Alexander Chong , Doggett Stephen L. , Lee Chow-Yang
Published: September 2020

We evaluated the influences of insecticide exposure time and mortality assessment intervals on the bioassay results of three insecticide-resistant tropical bed bug Cimex hemipterus (F.) populations (Madam Mo, Tanjong Tokong, and Green Lane). This was achieved using the surface contact method and tested with six commercial insecticide products: Tandem, Temprid SC, Pesguard FG161, Sumithrin, Sumithion, and Phantom applied at label rate on glass petri dishes. Six exposure times (5 min, 10 min, 30 min, 1 h, 4 h, and continuous exposure for 4 or 14 days for Phantom) were tested. A susceptible common bed bug Cimex lectularius L. strain (Monheim) was used as the reference strain as no susceptible C. hemipterus strain exists. In treatment with Temprid SC, the Tanjong Tokong strain showed significantly higher KT50 values at 5, 10, and 30 min exposures than 1 h, 4 h, and continuous exposures. When all resistant populations were evaluated at continuous exposure to Sumithion, they demonstrated moderate resistance levels (RR50 ranged from 6.0 to 7.9), while KT50 and KT95 of other shorter exposure times failed to be generated due to low knockdown rate. Higher mortalities were observed in Tanjong Tokong and Green Lane strains when tested at longer exposure times with Temprid SC, Pesguard FG161, Sumithrin, Sumithion, and Phantom. Better killing effect was observed in the treatment with Temprid SC (Tanjong Tokong and Green Lane strains), Pesguard FG161 (Tanjong Tokong and Green Lane strains), Sumithrin (all C. hemipterus strains), Sumithion (all C. hemipterus strains), and Phantom (all strains tested) at longer mortality assessment intervals. We demonstrated that insecticide exposure time and mortality assessment interval could potentially affect outcomes of product performance evaluations, resulting in underestimation or overestimation of insecticide resistance levels in field populations.
Assessment of Cosmeceutical Potentials of Selected Mushroom Fruitbody Extracts Through Evaluation of Antioxidant, Anti-Hyaluronidase and Anti-Tyrosinase Activity
Authors: Abd Razak Dang Lelamurni , Jamaluddin Anisah , Abd Rashid Nur Yuhasliza , Sani Nor Ajila , Abdul Manan Musaalbakri
Published: September 2020

Cosmeceutical formulations containing naturally derived active ingredients are currently preferred by consumers worldwide. Mushrooms are one of the potential sources for cosmeceutical ingredients but relevant research is still lacking. In this study, hot- and cold-water extractions were performed on four locally-cultivated mushrooms—Pleurotus ostreatus, Ganoderma lucidum, Auricularia polytricha and Schizophyllum commune—with the aim to assess the cosmeceutical potential of these mushroom fruitbody extracts. Total phenolics, polysaccharide and glucan content were determined. Antioxidant property of the mushroom extracts was assessed by determining the DPPH radical scavenging, ferric-reducing (FRAP) and superoxide anion (SOA) scavenging activity. Anti-hyaluronidase activity was used as an indicator for the anti-aging and anti-inflammatory property, while anti-tyrosinase activity was evaluated to assess the anti-pigmentation or whitening property of these extracts. Our results showed that total polysaccharide content of P. ostreatus extracts was the highest (235.8–253.6 mg GE/g extract), while extracts from G. lucidum contained the lowest glucan (10.12–10.67%). Cold-water extract from S. commune exhibited substantial tyrosinase inhibition activity (98.15%) and SOA scavenging activity (94.82%). The greatest hyaluronidase activity was exhibited by G. lucidum hot-water extract, with the value of 72.78%. The findings from the correlation analyses suggest that the cosmeceutical properties of these mushrooms can be attributed mainly to the combination of different types of compound such as polysaccharides and phenolics. Overall, cold-water extract of S. commune and hot-water extract of G. lucidum showed the best results and may be further investigated.
Influence of SPV Installations on the Thermal Character of the Urban Milieu
Authors: Matai Khushal
Published: September 2020

The solar photovoltaic (SPV) market is growing at a rapid pace with ambitious targets being set worldwide. India is not far behind with an overall solar target of 100 gigawatts (GW) to be achieved by 2022, out of which 40 gigawatts is to be achieved by solar rooftop. Additionally, the depleting non-renewable energy sources and the extensive pollution being done by the aforementioned sources are fueling the renewable energy drive. The threat of climate change, which is fast becoming a reality with effects seen globally, is another contributing factor. The effect of SPV installations on the temperature profiles of their surroundings and the urban thermal environment (UTE) is being studied at a global level, which has arrived at contradictory results, positive as well as negative. However, no such study has been done in the Indian context, which is crucial considering the country’s targets for rooftop installation specifically. The thermal environment of the vicinity is affected by the installations, as seen in the various global studies; the question is how this heat–energy balance is occurring in the Indian context. This review paper looks critically at studies focusing on the relation between SPV installation and the urban heat island (UHI) effect. It is a compilation and analysis of 22 different studies done so far at the global level to gain a thorough understanding of the diverse results. In conclusion, this review highlights the absence of any comprehensive study on the interaction of SPV installations with the built environment at a micro-level and establishes the need for region-based complete studies on the thermal behavior of SPV technology.
Increased Risk of Temporomandibular Joint Disorder in Patients with Rheumatoid Arthritis: A Longitudinal Follow-Up Study
Authors: Byun Soo-Hwan , Min Chanyang , Choi Hyo-Geun , Hong Seok-Jin
Published: September 2020

We evaluated the incidence of temporomandibular disorder (TMD) in patients with rheumatoid arthritis (RA) and examined the association between TMD and RA, through longitudinal follow-up. Population data from the Korean National Health Insurance Service-Health Screening Cohort from 2002 to 2015 was used. From 514,866 subjects, 3122 with RA were matched with 12,488 controls in a 1:4 ratio. The crude and adjusted models (for obesity, smoking, alcohol consumption, blood pressure, blood glucose, total cholesterol, and Charlson Comorbidity Index scores) were calculated. Chi-square tests, Kaplan-Meier (KM) analysis, and two-tailed analyses were used for statistical analysis. Stratified Cox proportional hazard models were used to assess the hazard ratios (HR) and 95% confidence intervals (CI) for TMD in the RA group, compared to those in the control group. The adjusted HR for TMD in RA was 2.52 (95% CI = 1.70–3.74), compared to the control group. The results were consistent with the subgroup analyses, according to age and sex, except in men older than 60 years of age. KM analysis showed similar results. Hence, we found that patients with RA have a higher risk of TMD, and should be observed for symptoms of the initial stage of TMD to prevent the risk of aggravation.
Endoscopic Reflux Esophagitis and Reflux-Related Symptoms after Helicobacter pylori Eradication Therapy: Meta-Analysis
Authors: Sugimoto Mitsushige , Murata Masaki , Mizuno Hitomi , Iwata Eri , Nagata Naoyoshi , Itoi Takao , Kawai Takashi
Published: September 2020

Backgrounds: The etiology of gastroesophageal reflux disease (GERD) including reflux esophagitis and non-erosive reflux disease is multifactorial and a recent meta-analysis showed no association between the development of GERD and Helicobacter pylori eradication in both Western and East-Asian populations. However, the problem remains that various inclusion criteria are used in these studies, which hinders meta-analysis. With a focus on reflux esophagitis with endoscopic mucosal injury, we meta-analysed to evaluate the association between eradication and reflux esophagitis and symptoms using a clearly defined set of inclusion criteria. Methods: We conducted a meta-analysis of studies published up until March 2020, which compared the incidence of reflux esophagitis and symptoms between patients undergoing H. pylori eradication therapy in a randomized placebo-controlled trial (Category A); between patients with successful and failed eradication (Category B); and between patients with successful vs. failed eradication, receipt of placebo, or no-treatment H. pylori-positives (Category C). Results: A total of 27 studies were included. Significant statistical effects were found for development of endoscopic reflux esophagitis [relative risk (RR): 1.46, 95% confidence interval (CI): 1.16–1.84, p = 0.01] or de novo reflux esophagitis (RR: 1.42, 95% CI: 1.01–2.00, p = 0.03) in the case group that received eradication in all studies, especially in Western populations. There was no significant difference in the incidence of symptoms after eradication between patient and control groups, regardless of category, location of population, or baseline disease. Conclusions: Eradication therapy for H pylori increases the risk of reflux esophagitis, irrespective of past history of esophagitis. In contrast, no effect was seen on reflux-related symptoms.