bluuebunny/crossref_metadata_2025 huggingface dataset:
COVID-19 Impacts on the IT Job Market: A Massive Job Ads Analysis
Authors: Cerioli Maura , Leotta Maurizio , Ricca Filippo
Published: August 2023

The Covid-19 pandemic has had a significant impact on many economic sectors. The most severe immediate financial effects of Covid-19 include job losses and decreased hiring, and we can expect generalized lower economic growth in the long term. While such phenomena are there for all to see, measuring their scope is complex. In this work, we performed a massive analysis of job postings (ads) taken from LinkUp, a popular job search web platform, to better understand the occupational trends in IT. We analyzed about nine million ads for computer and mathematical experts to measure the impact of the virus on the IT job market. We also extended our investigations to almost 109 million advertisements (about 300 GB of data) for all kinds of positions to overview the effects of Covid-19 on the job market at large. The results show that the Covid-19 crisis hit the job market during the first two quarters of 2020, causing the number of job advertisements to drop across all sectors (except one). Specifically, the IT sector lost between 15% and 48% of the ads, depending on the specific professional figure. Since the last quarter of 2020, the ad numbers have recovered for some sectors, and by the first 2021 quarter, all of them have more job ads than in the last five years. Finally, we used text analysis to understand the trends of interest in teleworking. We found that in the second quarter of 2020, the number of ads explicitly mentioning telework was almost three times the average of the previous quarters.
Multi-Modality Tensor Fusion Based Human Fatigue Detection
Authors: Ha Jongwoo , Ryu Joonhyuck , Ko Joonghoon
Published: August 2023

Multimodal learning is an expanding research area and aims to pursue a better understanding of given data by regarding different modals. Multimodal approaches for qualitative data are used for the quantitative proofing of ground-truth datasets and discovering unexpected phenomena. In this paper, we investigate the effect of multimodal learning schemes of quantitative data to assess its qualitative state. We try to interpret human fatigue levels through analyzing video, thermal image and voice data together. The experiment showed that the multimodal approach using three types of data was more effective than the method of using each dataset individually. As a result, we identified the possibility of predicting human fatigue states.
Alnus Airborne Pollen Trends during the Last 26 Years for Improving Machine Learning-Based Forecasting Methods
Authors: Novo-Lourés María , Fernández-González María , Pavón Reyes , Espinosa Kenia C. Sánchez , Laza Rosalía , Guada Guillermo , Méndez José R. , Fdez-Riverola Florentino , Rodríguez-Rajo Francisco Javier
Published: August 2023

Black alder (Alnus glutinosa (L.) Gaertn.) is a species of tree widespread along Europe and belongs to mixed hardwood forests. In urban environments, the tree is usually located along watercourses, as is the case in the city of Ourense. This taxon belongs to the betulaceae family, so it has a high allergenic potential in sensitive people. Due to the high allergenic capacity of this pollen type and the increase in global temperature produced by climate change, which induces a greater allergenicity, the present study proposes the implementation of a Machine Learning (ML) model capable of accurately predicting high-risk periods for allergies among sensitive people. The study was carried out in the city of Ourense for 28 years and pollen data were collected by means of the Hirst trap model Lanzoni VPPS-2000. During the same period, meteorological data were obtained from the meteorological station of METEOGALICIA in Ourense. We observed that Alnus airborne pollen was present in the study area during winter months, mainly in January and February. We found statistically significant trends for the end of the main pollen season with a lag trend of 0.68 days per year, and an increase in the annual pollen integral of 112 pollen grains per year and approximately 12 pollen grains/m3 per year during the pollen peak. A Spearman correlation test was carried out in order to select the variables for the ML model. The best ML model was Random Forest, which was able to detect those days with medium and high labels.
Tetraploid Induction with Leaf Morphology and Sunburn Variation in Sorbus pohuashanensis (Hance) Hedl
Authors: Zhang Zeren , Zhang Yan , Di Zexin , Zhang Ruili , Mu Yanjuan , Sun Tao , Tian Zhihui , Lu Yizeng , Zheng Jian
Published: August 2023

Sorbus pohuashanensis (Hance) Hedl. is an important forestry species valued for its ornamental, medicinal, and ecological properties. Polyploidy breeding is an important method of germplasm innovation; however, polyploidy induction and phenotypic variation caused by chromosome doubling in S. pohuashanensis are poorly understood. In this study, S. pohuashanensis seeds were used to explore the effects of different colchicine concentrations, cold stratification times, and seeds from different sources on polyploidy induction. Ploidy levels of the regenerated plants were determined by flow cytometry. The results showed that the tetraploid induction effect of S. pohuashanensis seeds was significantly affected by colchicine concentration, and the highest tetraploid induction rate of 24.75% was achieved by immersion in 0.2% (w/v) colchicine for 48 h. After 2 years of induction, 77 tetraploid plants were obtained. Compared to diploids, tetraploid plants showed significant variations in plant height, leaf morphology (apical leaflet width, middle leaflet width), and diameter of the middle petiole. The stomatal size and chloroplast number increased with chromosome doubling whereas the stomatal number and density decreased. In addition, significant differences in the percentage of sunburn associated with ploidy changes were observed. This study provides a technique for tetraploid induction of S. pohuashanensis seeds, showing the variation in traits caused by polyploidization and the effect of chromosome doubling on sunburn resistance. Tetraploidy induction provides a new direction for S. pohuashanensis germplasm innovation.
Effects of Microencapsulated Blend of Organic Acids and Pure Botanicals on the Ruminal Microbiota in an In Vitro Dual-Flow Continuous Culture System
Authors: Lobo Richard R. , Roesch Luiz Fernando Wurdig , Grilli Ester , Faciola Antonio P.
Published: August 2023

The objective of the study was to evaluate the effects of dietary supplementation of a microencapsulated blend of organic acids and pure botanicals (mOAPBs) on the solid- and liquid-associated microenvironment (SAM and LAM, respectively) of the ruminal microbiome using an in vitro dual-flow continuous culture system. Ruminal content was incubated in eight fermenters and the basal diet was supplemented with increasing levels of mOAPBs (0; 0.12; 0.24; or 0.36% DM) which contained 55.6% hydrogenated and refined palm oil, 25% citric acid, 16.7% sorbic acid, 1.7% thymol, and 1% vanillin. All diets had a similar nutritional composition (16.1 CP, 30.9 NDF, and 32.0 starch, % DM basis). After 7 days of adaptation, a pooled sample across the days was collected in each period for identification of the microbiome of SAM and LAM. There was no effect of mOAPB on alpha-, beta-diversity, and microbial abundance. The SAM had a greater bacterial diversity and the principal component analysis demonstrated that it had a divergent bacterial profile from LAM. Additionally, SAM had an increased abundance of carbohydrate-degrading microorganisms. In summary, mOAPBs did not modulate the ruminal microbiome. The microenvironment microbiome of solid- and liquid-associated microenvironments were different, with SAM having a greater carbohydrate-degrading microorganism population.
Effects of Cow’s Milk Processing on MicroRNA Levels
Authors: Abou el qassim Loubna , Martínez Beatriz , Rodríguez Ana , Dávalos Alberto , López de las Hazas María-Carmen , Menéndez Miranda Mario , Royo Luis J.
Published: August 2023

MicroRNAs (miRNAs) regulate gene expression and might resist adverse physicochemical conditions, which makes them potential biomarkers. They are being investigated as biomarkers of dairy production systems, based on the variations in their levels in raw milk depending on animal diet and management. Whether miRNA levels can serve as biomarkers for dairy products remains unclear, since technological or culinary treatments, such as fermentation, may alter their levels. Here, 10 cow dairy farms were sampled in Asturias (north-west Spain) and milk samples were subjected to microwave heating or used to produce yogurt or cheese. Total RNA was isolated from raw milk and three derived products, and levels of seven miRNAs, selected based on previous studies as possible milk production system biomarkers, were assessed by RT-qPCR. The treatments decreased levels of all miRNAs to some extent. These results also imply that cheesemaking increases the concentration of miRNAs in this product; raw milk and cheese supposedly may provide similar concentrations of miRNAs, higher than those of yogurt and microwaved milk. They also indicate that the content of certain miRNAs in raw milk cannot necessarily be extrapolated to other dairy products.
Development and Characterization of Antioxidant and Antibacterial Films Based on Potato Starch Incorporating Viola odorata Extract to Improve the Oxidative and Microbiological Quality of Chicken Fillets during Refrigerated Storage
Authors: Nikmanesh Ali , Baghaei Homa , Mohammadi Nafchi Abdorreza
Published: August 2023

In this research, the antioxidant and antibacterial activities of active films based on potato starch containing Viola odorata extract (VOE) were investigated both in vitro and in chicken fillets. The VOE was added to the starch film formulation at 0, 1, 2, and 3% (w/v). The results showed that by increasing the extract level, the total phenol content and antioxidant and antibacterial activity of the films against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium improved remarkably. The results of the meat tests indicated the significant antioxidant and antimicrobial activity of active films containing different levels of VOE in chicken fillets, and a direct relationship was observed between the concentration of the extract and the functional activity of the films, so with the increase in the concentration of the extract in the films, the rate of lipid oxidation and growth of microorganisms in the chicken fillets decreased significantly during the storage period, and less volatile nitrogen bases, metmyoglobin, and oxidation products were produced in the fillets. In general, the results of this research demonstrated that an active film based on potato starch containing VOE (especially 2 and 3% levels) has the ability to extend the oxidative and microbiological shelf life of chicken fillets during cold storage for at least eight days.
Prevalence of Listeria monocytogenes in RTE Meat Products of Quevedo (Ecuador)
Authors: Meza-Bone Gary Alex , Meza Bone Jessica Sayonara , Cedeño Ángel , Martín Irene , Martín Alberto , Maddela Naga Raju , Córdoba Juan J.
Published: August 2023

Listeria monocytogenes is a foodborne pathogen that causes listeriosis and can be a problem in areas where meat products are sold at unregulated storage temperatures. In this work, the prevalence of L. monocytogenes was determined in the five most widely traded meat products in the province of Quevedo (Ecuador): bacon, “chorizo paisa”, grilled hamburger meat, mortadella, and salami. A total of 1000 samples of these products were analyzed in two seasons of the year (dry season/rainy season). All L. monocytogenes isolates were confirmed by PCR with primers designed for the iap gene. Furthermore, the positive samples were quantified for L. monocytogenes. Of the 1000 meat products analyzed, 163 were positive for L. monocytogenes (16.3%). The prevalence of L. monocytogenes in the two seasons in different meat products was as follows: 22.5% in mortadella, 19% in hamburger meat, 15% in bacon, 14.5% in chorizo paisa and 10.5% in salami. In addition, the concentration of L. monocytogenes in most of the positive samples was in the range of 4–6 log CFU/g or even higher. The results show the need for improvements in the hygienic measures and meat storage temperatures in Quevedo (Ecuador) to avoid risks of foodborne listeriosis.
Application of Hyperspectral Imaging as a Nondestructive Technology for Identifying Tomato Maturity and Quantitatively Predicting Lycopene Content
Authors: Dai Chunxia , Sun Jun , Huang Xingyi , Zhang Xiaorui , Tian Xiaoyu , Wang Wei , Sun Jingtao , Luan Yu
Published: August 2023

Maturity is a crucial indicator in assessing the quality of tomatoes, and it is closely related to lycopene content. Using hyperspectral imaging, this study aimed to monitor tomato maturity and predict its lycopene content at different maturity stages. Standard normal variable (SNV) transformation was applied to preprocess the hyperspectral data. Then, using competitive adaptive reweighted sampling (CARS), the characteristic wavelengths were selected to simplify the calibration models. Based on the full and characteristic wavelengths, a support vector classifier (SVC) model was developed to determine tomato maturity qualitatively. The results demonstrated that the classification accuracy using the characteristic wavelength led to the obtention of better results with an accuracy of 95.83%. In addition, the support vector regression (SVR) and partial least squares regression (PLSR) models were utilized to predict lycopene content. With a coefficient of determination for prediction (R2P) of 0.9652 and a root mean square error for prediction (RMSEP) of 0.0166 mg/kg, the SVR model exhibited the best quantitative prediction capacity based on the characteristic wavelengths. Following this, a visual distribution map was created to evaluate the lycopene content in tomato fruit intuitively. The results demonstrated the viability of hyperspectral imaging for detecting tomato maturity and quantitatively predicting the lycopene content during storage.
Examination of Factors Affecting Site-Directed RNA Editing by the MS2-ADAR1 Deaminase System
Authors: Azad Md Thoufic Anam , Qulsum Umme , Tsukahara Toshifumi
Published: August 2023

Adenosine deaminases acting on RNA (ADARs) have double-stranded RNA binding domains and a deaminase domain (DD). We used the MS2 system and specific guide RNAs to direct ADAR1-DD to target adenosines in the mRNA encoding-enhanced green fluorescence protein. Using this system in transfected HEK-293 cells, we evaluated the effects of changing the length and position of the guide RNA on the efficiency of conversion of amber (TAG) and ochre (TAA) stop codons to tryptophan (TGG) in the target. Guide RNAs of 19, 21 and 23 nt were positioned upstream and downstream of the MS2-RNA, providing a total of six guide RNAs. The upstream guide RNAs were more functionally effective than the downstream guide RNAs, with the following hierarchy of efficiency: 21 nt > 23 nt > 19 nt. The highest editing efficiency was 16.6%. Off-target editing was not detected in the guide RNA complementary region but was detected 50 nt downstream of the target. The editing efficiency was proportional to the amount of transfected deaminase but inversely proportional to the amount of the transfected guide RNA. Our results suggest that specific RNA editing requires precise optimization of the ratio of enzyme, guide RNA, and target RNA.
The Eye as a Symbol of Ill-Fatedness in Two Canonical Picaresque Works: Lazarillo de Tormes and Guzmán de Alfarache
Authors: Ellis Sarah Louise
Published: August 2023

It seemed unimaginable that the eye, denoting visuality and deemed accurate and reliable in accordance with Aristotelian theories in circulation during the Spanish Golden Age could be considered as anything other than a revered hallmark of guidance and intellect. Nevertheless, the literary phenomenon) of the picaresque emerged at the onset of the seventeenth century to defy the chivalric and pastoral fantasies that were masking the real anxieties faced by an era of decline. The picaresque genre brought warning that turning a blind eye to Spain’s already-waning fortunes could not last forever. Yet, by doing so, it lent favour to such blindness, underlining how the eye, both symbolically and substantially, actually evoked a sense of ill-fatedness and misfortune. This paper calls for an exploration of how an ominous utilisation of the eye is presented in the most canonical picaresque works: Lazarillo de Tormes and Mateo Alemán’s Guzmán de Alfarache. From the imperative role of the blind man in opening the eyes of the young protagonist, to the doomed interpolated cosplay of seeing and unseeing throughout Lazarillo’s trajectory, and from Guzmán’s receptivity to appearances and Alemán’s lending of visual lexicon to his picaro protagonist, one must ask: how and why does the bodily organ of the eye, through both notion and function, serve as a depiction of hardship and disaster within these picaresque texts, and how does it reflect the overarching societal views towards intellect and religion during this epoch of “ocularcentrism”?
Sports and Special Populations: Training Physiology in Health and Sports Performance
Authors: Aidar Felipe J.
Published: August 2023

Health is increasingly being studied, notably concerning preventive measures for health issues [...]
Uncovering the Effect of News Signals on Daily Stock Market Performance: An Econometric Analysis
Authors: Raza Shahid , Baiqing Sun , Kay-Khine Pwint , Ali Kemal Muhammad
Published: August 2023

The stock markets in developing countries are highly responsive to breaking news and events. Our research explores the impact of economic conditions, financial policies, and politics on the KSE-100 index through daily market news signals. Utilizing simple OLS regression and ARCH/GARCH regression methods, we determine the best model for analysis. The results reveal that political and global news has a significant impact on KSE-100 index. Blue chip stocks are considered safer investments, while short-term panic responses often overshadow rational decision-making in the stock market. Investors tend to quickly react to negative news, making them risk-averse. Our findings suggest that the ARCH/GARCH models are better at predicting stock market fluctuations compared to the simple OLS method.
Modeling Theory of Mind in Dyadic Games Using Adaptive Feedback Control
Authors: Freire Ismael T. , Arsiwalla Xerxes D. , Puigbò Jordi-Ysard , Verschure Paul
Published: August 2023

A major challenge in cognitive science and AI has been to understand how intelligent autonomous agents might acquire and predict the behavioral and mental states of other agents in the course of complex social interactions. How does such an agent model the goals, beliefs, and actions of other agents it interacts with? What are the computational principles to model a Theory of Mind (ToM)? Deep learning approaches to address these questions fall short of a better understanding of the problem. In part, this is due to the black-box nature of deep networks, wherein computational mechanisms of ToM are not readily revealed. Here, we consider alternative hypotheses seeking to model how the brain might realize a ToM. In particular, we propose embodied and situated agent models based on distributed adaptive control theory to predict the actions of other agents in five different game-theoretic tasks (Harmony Game, Hawk-Dove, Stag Hunt, Prisoner’s Dilemma, and Battle of the Exes). Our multi-layer control models implement top-down predictions from adaptive to reactive layers of control and bottom-up error feedback from reactive to adaptive layers. We test cooperative and competitive strategies among seven different agent models (cooperative, greedy, tit-for-tat, reinforcement-based, rational, predictive, and internal agents). We show that, compared to pure reinforcement-based strategies, probabilistic learning agents modeled on rational, predictive, and internal phenotypes perform better in game-theoretic metrics across tasks. The outlined autonomous multi-agent models might capture systems-level processes underlying a ToM and suggest architectural principles of ToM from a control-theoretic perspective.
Edible Insects: A Historical and Cultural Perspective on Entomophagy with a Focus on Western Societies
Authors: Olivadese Marianna , Dindo Maria Luisa
Published: August 2023

The relationship between insects and humans throughout history has always been complex and multifaceted. Insects are both a source of fascination and fear for humans and have played important roles in human culture, economy, and health. Nowadays, there is growing interest in using insects as a sustainable and environmentally friendly source of protein and other nutrients. Entomophagy can be seen as a new opportunity for the food industry and global food security. In fact, insects require far fewer resources than traditional livestock, and there are many references to insect consumption in human history. The ancient Romans are known to have eaten various insects, including beetles, caterpillars, and locusts. Insects such as crickets, grasshoppers, and ants have been eaten for centuries and are still considered a delicacy in many parts of the world, especially in Africa, Asia, Latin America, and Oceania. Entomophagy has, thus, been a part of human history for thousands of years and continues to be an important food habit for many people around the world. These topics are explored in this article from a historical and cultural perspective (e.g., ecological, nutritional, spiritual, and socio-psychological), with a focus on the progressive acceptance of edible insects in Western societies, since this novel food has also its roots in the Western world.
Functional Divergence of the Tribolium castaneum engrailed and invected Paralogs
Authors: Blunk Summer , Garcia-Verdugo Hector , O’Sullivan Sierra , Camp James , Haines Michael , Coalter Tara , Williams Terri A. , Nagy Lisa M.
Published: August 2023

Engrailed (en) and invected (inv) encode paralogous transcription factors found as a closely linked tandem duplication within holometabolous insects. Drosophila en mutants segment normally, then fail to maintain their segments. Loss of Drosophila inv is viable, while loss of both genes results in asegmental larvae. Surprisingly, the knockdown of Oncopeltus inv can result in the loss or fusion of the entire abdomen and en knockdowns in Tribolium show variable degrees of segmental loss. The consequence of losing or knocking down both paralogs on embryogenesis has not been studied beyond Drosophila. To further investigate the relative functions of each paralog and the mechanism behind the segmental loss, Tribolium double and single knockdowns of en and inv were analyzed. The most common cuticular phenotype of the double knockdowns was small, limbless, and open dorsally, with all but a single, segmentally iterated row of bristles. Less severe knockdowns had fused segments and reduced appendages. The Tribolium paralogs appear to act synergistically: the knockdown of either Tribolium gene alone was typically less severe, with all limbs present, whereas the most extreme single knockdowns mimic the most severe double knockdown phenotype. Morphological abnormalities unique to either single gene knockdown were not found. inv expression was not affected in the Tribolium en knockdowns, but hh expression was unexpectedly increased midway through development. Thus, while the segmental expression of en/inv is broadly conserved within insects, the functions of en and inv are evolving independently in different lineages.
Evaluation of Clinical Manifestations of Hemorrhoidal Disease, Carried Out Surgeries and Prolapsed Anorectal Tissues: Associations with ABO Blood Groups of Patients
Authors: Fišere Inese , Groma Valērija , Svirskis Šimons , Strautmane Estere , Gardovskis Andris
Published: August 2023

Hemorrhoidal disease (HD) is a chronic multifactorial disease. Increased abdominal pressure, along with hyperperfusion, neovascularization, overexpression of inflammatory mediators, and dysbiosis, contributes to the development of HD. The deterioration of the anchoring connective tissue with reduced collagen content and altered collagen ratios, dilatation of blood vessels and thrombosis, muscle injury, and inflammation gradually lead to clinically manifesting prolapse and bleeding from hemorrhoids. The associations of the ABO blood types with a disease have been investigated for the upper gastrointestinal tract only. This study aimed to evaluate HD clinical manifestations, surgeries carried out, and the status of prolapsed anorectal tissues by exploring the associations with the patients’ ABO blood groups. Clinical and various morphological methods, combined with extensive bioinformatics, were used. The blood type 0, grade III and IV HD individuals constituted the largest group in a moderately-sized cohort of equally represented males and females studied and submitted to surgical treatment of hemorrhoids. There were significantly more complaints reported by HD females compared to males (p = 0.0094). The Longo technique appeared mostly used, and there were proportionally more surgeries performed below the dentate line for HD individuals with blood type 0 compared to other blood type patients (24% vs. 11%). HD males were found to present with significantly more often inflamed rectal mucosa (p < 0.05). Loosening and weakening of collagenous components of the rectal wall combined with vascular dilation and hemorrhage was found to differ in 0 blood type HD individuals compared to other types. HD males were demonstrated to develop the ruptures of vascular beds significantly more often when compared to HD females (p = 0.0165). Furthermore, 0 blood type HD males were significantly more often affected by a disease manifested with tissue hemorrhage compared to the 0 blood type HD females (p = 0.0081). Collectively, the local status of chronically injured anorectal tissue should be considered when applying surgical techniques. Future studies could include patients with HD grades I and II to gain a comprehensive understanding of the disease progression, allowing for a comparison of tissue changes at different disease stages.
Medicare Advantage in Soft Tissue Sarcoma May Be Associated with Worse Patient Outcomes
Authors: Wang Jennifer C. , Liu Kevin C. , Gettleman Brandon S. , Piple Amit S. , Chen Matthew S. , Menendez Lawrence R. , Heckmann Nathanael D. , Christ Alexander B.
Published: August 2023

Medicare Advantage healthcare plans may present undue impediments that result in disparities in patient outcomes. This study aims to compare the outcomes of patients who underwent STS resection based on enrollment in either traditional Medicare (TM) or Medicare Advantage (MA) plans. The Premier Healthcare Database was utilized to identify all patients ≥65 years old who underwent surgery for resection of a lower-extremity STS from 2015 to 2021. These patients were then subdivided based on their Medicare enrollment status (i.e., TM or MA). Patient characteristics, hospital factors, and comorbidities were recorded for each cohort. Bivariable analysis was performed to assess the 90-day risk of postoperative complications. Multivariable analysis controlling for patient sex, as well as demographic and hospital factors found to be significantly different between the cohorts, was also performed. From 2015 to 2021, 1858 patients underwent resection of STS. Of these, 595 (32.0%) had MA coverage and 1048 (56.4%) had TM coverage. The only comorbidities with a significant difference between the cohorts were peripheral vascular disease (p = 0.027) and hypothyroidism (p = 0.022), both with greater frequency in MA patients. After controlling for confounders, MA trended towards having significantly higher odds of pulmonary embolism (adjusted odds ratio (aOR): 1.98, 95% confidence interval (95%-CI): 0.58–6.79), stroke (aOR: 1.14, 95%-CI: 0.20–6.31), surgical site infection (aOR: 1.59, 95%-CI: 0.75–3.37), and 90-day in-hospital death (aOR 1.38, 95%-CI: 0.60–3.19). Overall, statistically significant differences in postoperative outcomes were not achieved in this study. The authors of this study hypothesize that this may be due to study underpowering or the inability to control for other oncologic factors not available in the Premier database. Further research with higher power, such as through multi-institutional collaboration, is warranted to better assess if there truly are no differences in outcomes by Medicare subtype for this patient population.
Early Perturbations in Red Blood Cells in Response to Murine Malarial Parasite Infection: Proof-of-Concept 1H NMR Metabolomic Study
Authors: Sengupta Arjun , Ghosh Soumita , Sharma Shobhona , Sonawat Haripalsingh
Published: August 2023

Background: The major focus of metabolomics research has been confined to the readily available biofluids—urine and blood serum. However, red blood cells (RBCs) are also readily available, and may be a source of a wealth of information on vertebrates. However, the comprehensive metabolomic characterization of RBCs is minimal although they exhibit perturbations in various physiological states. RBCs act as the host of malarial parasites during the symptomatic stage. Thus, understanding the changes in RBC metabolism during infection is crucial for a better understanding of disease progression. Methods: The metabolome of normal RBCs obtained from Swiss mice was investigated using 1H NMR spectroscopy. Several 1 and 2-dimensional 1H NMR experiments were employed for this purpose. The information from this study was used to investigate the changes in the RBC metabolome during the early stage of infection (~1% infected RBCs) by Plasmodium bergheii ANKA. Results: We identified over 40 metabolites in RBCs. Several of these metabolites were quantitated using 1H NMR spectroscopy. The results indicate changes in the choline/membrane components and other metabolites during the early stage of malaria. Conclusions: The paper reports the comprehensive characterization of the metabolome of mouse RBCs. Changes during the early stage of malarial infection suggest significant metabolic alteration, even at low parasite content (~1%). General significance: This study should be of use in maximizing the amount of information available from metabolomic experiments on the cellular components of blood. The technique can be directly applied to real-time investigation of infectious diseases that target RBCs.
Association of Platelet-Monocyte Ratio with Dyslipidemia in Saudi Arabia: A Large, Population-Based Study
Authors: Alfhili Mohammad A. , Alotaibi Ghada A. , Alfaifi Mohammed , Almoghrabi Yousef , Alsughayyir Jawaher
Published: August 2023

Background: Abnormal lipid metabolism predisposes to cardiovascular disease. However, dyslipidemia is often asymptomatic leading to its underdiagnosis. Therefore, it is of utmost importance to identify biomarkers that reflect an abnormal lipid profile and trigger the specific investigation of lipid metabolism. The platelet–monocyte ratio (PMR) is a severely understudied index whose association with disturbed lipid markers remains unknown. Methods: A cross-sectional study of the association between PMR and comprehensive lipid profile including total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG), TC/HDL, LDL/HDL, and TG/HDL in 14,269 Saudi subjects was designed. Prevalence, risk measures, association, and the diagnostic performance (i.e., area under the curve (AUC)) were evaluated. Results: Median PMR was significantly elevated in subjects with high TC (p < 0.01), TG, TC/HDL, LDL/HDL, TG/HDL, and LDL and reduced in those with low HDL (all p < 0.0001) compared to normal subjects. The increase in PMR was abolished when only males with high TC were considered. Except for TC and LDL, all other abnormal markers were significantly more prevalent when PMR was lower (higher for HDL) than a certain cutoff specific for each parameter. Moreover, the odds of having PMR readings above or below the selected cutoffs are significantly higher with all lipid abnormalities. PMR was also weakly but significantly and differentially correlated with all forms of dyslipidemia (p < 0.0001). Notably, the highest diagnostic accuracy of PMR was observed for reduced HDL (AUC = 0.608, p < 0.0001) and elevated TG/HDL (AUC = 0.596, p < 0.0001). Conclusions: PMR is a novel, inexpensive, and readily available index that is associated with all forms of dyslipidemia, suggesting its potential use in related disorders.
Recent Advances in Biodegradable and Biocompatible Synthetic Polymers Used in Skin Wound Healing
Authors: Xu Ruojiao , Fang Yifeng , Zhang Zhao , Cao Yajie , Yan Yujia , Gan Li , Xu Jinbao , Zhou Guoying
Published: August 2023

The treatment of skin wounds caused by trauma and pathophysiological disorders has been a growing healthcare challenge, posing a great economic burden worldwide. The use of appropriate wound dressings can help to facilitate the repair and healing rate of defective skin. Natural polymer biomaterials such as collagen and hyaluronic acid with excellent biocompatibility have been shown to promote wound healing and the restoration of skin. However, the low mechanical properties and fast degradation rate have limited their applications. Skin wound dressings based on biodegradable and biocompatible synthetic polymers can not only overcome the shortcomings of natural polymer biomaterials but also possess favorable properties for applications in the treatment of skin wounds. Herein, we listed several biodegradable and biocompatible synthetic polymers used as wound dressing materials, such as PVA, PCL, PLA, PLGA, PU, and PEO/PEG, focusing on their composition, fabrication techniques, and functions promoting wound healing. Additionally, the future development prospects of synthetic biodegradable polymer-based wound dressings are put forward. Our review aims to provide new insights for the further development of wound dressings using synthetic biodegradable polymers.
Experimental Research into the Uniaxial Compressive Strength of Low-Density Reef Limestone Based on Image Recognition
Authors: Wei Xiaoqing , Luo Yi , Tao Yuhang , Li Xinping , Meng Fei
Published: August 2023

Low-density reef limestone is widely distributed in tropical oceans; exploring its mechanical properties is of significance to practices in marine foundation engineering. In this research, laboratory experiments on low-density reef limestones with two different types of porous structures were conducted using image recognition methods to study the special mechanical properties of low-reef limestone. S¯ was defined as the parameter quantifying the pore geometry, and the calculation method of S¯ was optimized based on image recognition data. Finally, the influencing factors of uniaxial compressive strength (UCS) of low-density reef limestone were analyzed, and a modified formula considering pore structure was proposed. The results indicate the following: Image recognition methods were used to determine feasibility and convenience of capturing 2D pore geometric information of specimens. The optimization method of S¯ is conducive to improving automatic image recognition accuracy. Low-density reef limestones with different porous structures show small difference in porosity and density, while they exhibit large differences in pore sizes and UCS. The UCS of low-density reef limestone is found to be jointly influenced by pore structure and density (it increases with the decrease in parameter S¯ and increase in density). The results may provide help for those investigating the mechanical properties of reef limestone and practices in marine foundation engineering.
Performance Review of Strain-Hardening Cementitious Composites in Structural Applications
Authors: Xue Bingshuang , Xu Binbin , Lu Weihua , Zhang Yongxing
Published: August 2023

Strain-hardening cementitious composites (SHCC) are an attractive construction material with obvious advantages of large strain capacity and high strength, as well as excellent workability and easy processing using conventional equipment. Moreover, SHCC can be designed with varied mix proportions in order to satisfy various requirements and expectations to overcome the shortages of existing construction materials. However, the behavior of SHCC in the structural application is varied from that of SHCC material, which is reviewed and presented in this paper, focusing on the flexural and shear behavior of the SHCC member and the SHCC layer used for strengthening reinforced concrete (RC). The reviewed results demonstrate that both the zero-span tensile behavior of the stress concentration and the uniaxial tensile behavior of the bending effect can influence the crack propagation patterns of multiple fine cracks in the SHCC strengthening layer, in which the crack distribution within the SHCC layer is limited near the existing crack in the RC substrate member in the zero-span tensile behavior. Moreover, the crack propagation patterns of the SHCC strengthening layer are changed with varied layer thicknesses, and the SHCC strengthening layer, even with a small thickness, can significantly increase the shear load carrying capacity of the shear strengthened RC member. This work provides the foundations for promoting SHCC material in the structural application of repairing or retrofitting concrete structures.
A Novel Conditional Connectivity and Hamiltonian Connectivity of BCube with Various Faulty Elements
Authors: Lv Yali , Lin Cheng-Kuan , You Lantao
Published: August 2023

BCube is one of the main data center networks because it has many attractive features. In practical applications, the failure of components or physical connections is inevitable. In data center networks in particular, switch failures are unavoidable. Fault-tolerance capability is one main aspect to measure the performance of data center networks. Connectivity, fault tolerance Hamiltonian connectivity, and fault tolerance Hamiltonicity are important parameters that assess the fault tolerance of networks. In general, the distribution of fault elements is scattered, and it is necessary to consider the distribution of fault elements in different dimensions. We research the fault tolerance of BCube when considering faulty switches and faulty links/edges that distribute in different dimensions. We also investigate the connectivity, fault tolerance Hamiltonian connectivity, and Hamiltonicity. This study better evaluates the fault-tolerant performance of data center networks.
Edge-Based Minimal k-Core Subgraph Search
Authors: Wang Ting , Jiang Yu , Yang Jianye , Xing Lei
Published: August 2023

In social networks, k-core is commonly used to measure the stability of a network. When a user in a k-core leaves the network, other users may follow the user to leave. Hence, maintaining a key user is important to keep the stability of a network. It is known that an edge between two users models the relationship between the two users. In some scenarios, maintaining a relationship comes at a cost. Therefore, selectively in maintaining the relationships between users is crucial. In this paper, we for the first time conceive the concept of an edge-based minimal k-core model. An edge-based minimal k-core is a k-core with a minimal number of edges. In other words, removing any edge in an edge-based minimal k-core would make it not be a k-core any more. Based on this model, we proposed two problems, namely, an edge-based minimal k-core subgraph search (EMK-SS) and an edge-based minimal k-core subgraph search with a query node q (EMK-q-SS). Given a graph G, an integer k, and a query node (a key user) q, the EMK-q-SS problem is to find all the edge-based minimal k-cores containing the query node q, and the EMK-SS problem is to find all the edge-based minimal k-cores. We also theoretically prove that the two problems are both NP-complete. To deal with the proposed problems, we design two novel algorithms, namely the edge deletion algorithm and edge extension algorithm. Further, a graph partitioning technique is employed to speed up the computation. Comprehensive experiments on synthetic and real networks are conducted to demonstrate the effect and efficiency of our proposed methods.
The Remediation of Dysprosium-Containing Effluents Using Cyanobacteria Spirulina platensis and Yeast Saccharomyces cerevisiae
Authors: Zinicovscaia Inga , Yushin Nikita , Grozdov Dmitrii , Peshkova Alexandra , Vergel Konstantin , Rodlovskaya Elena
Published: August 2023

Dysprosium is one of the most critical rare earth elements for industry and technology. A comparative study was carried out to assess the biosorption capacity of cyanobacteria Spirulina platensis and yeast Saccharomyces cerevisiae toward dysprosium ions. The effect of experimental parameters such as pH, dysprosium concentration, time of contact, and temperature on the biosorption capacity was evaluated. Biomass before and after dysprosium biosorption was analyzed using neutron activation analysis and Fourier-transform infrared spectroscopy. For both biosorbents, the process was quick and pH-dependent. The maximum removal of dysprosium using Spirulina platensis (50%) and Saccharomyces cerevisiae (68%) was attained at pH 3.0 during a one-hour experiment. The adsorption data for both biosorbents fitted well with the Langmuir isotherm model, whereas the kinetics of the process followed the pseudo-second-order and Elovich models. The maximum biosorption capacity of Spirulina platensis was 3.24 mg/g, and that of Saccharomyces cerevisiae was 5.84 mg/g. The thermodynamic parameters showed that dysprosium biosorption was a spontaneous process, exothermic for Saccharomyces cerevisiae and endothermic for Spirulina platensis. Biological sorbents can be considered an eco-friendly alternative to traditional technologies applied for dysprosium ion recovery from wastewater.
Adipose Tissue Hyperplasia and Hypertrophy in Common and Syndromic Obesity—The Case of BBS Obesity
Authors: Horwitz Avital , Birk Ruth
Published: August 2023

Obesity is a metabolic state generated by the expansion of adipose tissue. Adipose tissue expansion depends on the interplay between hyperplasia and hypertrophy, and is mainly regulated by a complex interaction between genetics and excess energy intake. However, the genetic regulation of adipose tissue expansion is yet to be fully understood. Obesity can be divided into common multifactorial/polygenic obesity and monogenic obesity, non-syndromic and syndromic. Several genes related to obesity were found through studies of monogenic non-syndromic obesity models. However, syndromic obesity, characterized by additional features other than obesity, suggesting a more global role of the mutant genes related to the syndrome and, thus, an additional peripheral influence on the development of obesity, were hardly studied to date in this regard. This review summarizes present knowledge regarding the hyperplasia and hypertrophy of adipocytes in common obesity. Additionally, we highlight the scarce research on syndromic obesity as a model for studying adipocyte hyperplasia and hypertrophy, focusing on Bardet–Biedl syndrome (BBS). BBS obesity involves central and peripheral mechanisms, with molecular and mechanistic alternation in adipocyte hyperplasia and hypertrophy. Thus, we argue that using syndromic obesity models, such as BBS, can further advance our knowledge regarding peripheral adipocyte regulation in obesity.
Underlying Mechanisms of Brain Aging and Neurodegenerative Diseases as Potential Targets for Preventive or Therapeutic Strategies Using Phytochemicals
Authors: Abdolmaleky Hamid , Zhou Jin-Rong
Published: August 2023

During aging, several tissues and biological systems undergo a progressive decline in function, leading to age-associated diseases such as neurodegenerative, inflammatory, metabolic, and cardiovascular diseases and cancer. In this review, we focus on the molecular underpinning of senescence and neurodegeneration related to age-associated brain diseases, in particular, Alzheimer’s and Parkinson’s diseases, along with introducing nutrients or phytochemicals that modulate age-associated molecular dysfunctions, potentially offering preventive or therapeutic benefits. Based on current knowledge, the dysregulation of microglia genes and neuroinflammation, telomere attrition, neuronal stem cell degradation, vascular system dysfunction, reactive oxygen species, loss of chromosome X inactivation in females, and gut microbiome dysbiosis have been seen to play pivotal roles in neurodegeneration in an interactive manner. There are several phytochemicals (e.g., curcumin, EGCG, fucoidan, galangin, astin C, apigenin, resveratrol, phytic acid, acacetin, daucosterol, silibinin, sulforaphane, withaferin A, and betulinic acid) that modulate the dysfunction of one or several key genes (e.g., TREM2, C3, C3aR1, TNFA, NF-kb, TGFB1&2, SIRT1&6, HMGB1, and STING) affected in the aged brain. Although phytochemicals have shown promise in slowing down the progression of age-related brain diseases, more studies to identify their efficacy, alone or in combinations, in preclinical systems can help to design novel nutritional strategies for the management of neurodegenerative diseases in humans.
Early Events after Herpes Simplex Virus-Type 1 Entry Are Necessary for the Release of Gamma-Hydroxybutyrate upon Acute Infection
Authors: Osinaga Faith O. , Chen Yu-Chih , Kharel Madan K. , Waguespack Yan , Li Sichu , Hsia Shaochung Victor
Published: August 2023

We reported that gamma-hydroxybutyrate (GHB) is released upon Herpes Simplex Virus Type-1 (HSV-1) acute infection. However, the cellular biochemical processes involved in the production of GHB in infected cells are unclear. This study aims to shed light on the biochemical pathway and the stage within the viral life cycle responsible for the release of GHB in infected cells. UV-inactivation, acyclovir (ACV), and cycloheximide (CHX) treatments were used to inhibit HSV-1 replication at various stages. Vero cells treated with UV-inactivated HSV-1 significantly decreased GHB production. However, ACV or CHX treatments did not affect GHB production. We also showed that inhibition of glycolytic enzyme enolase by sodium fluoride (NaF) significantly reduces GHB production upon infection. This finding suggests that suppression of glycolytic activity negatively affects cellular GHB production. Our data also indicated that succinic semialdehyde dehydrogenase, an enzyme involved in the shunt of the tricarboxylic acid (TCA) cycle to generate succinic acid, was decreased upon infection, suggesting that infection may trigger the accumulation of succinic semialdehyde, causing the production of GHB. Although the precise mechanism has yet to be defined, our results suggest that early events following infection modulates the release of GHB, which is generated through the metabolic pathways of glycolysis and TCA cycle.
13.5 μJ, 20 kHz Repetition Rate, Single Frequency Pr3+:YLF Master Oscillator Power Amplifier System
Authors: Dai Weicheng , Jin Long , Liu Chang , Dong Yuan , Jin Guangyong
Published: August 2023

This article describes a master oscillator and power amplifier (MOPA) system with a single longitudinal mode (SLM) and high-repetition-frequency Pr3+:YLF active medium that was end-pumped by two 444 nm laser diodes. The Pr3+:YLF MOPA laser system produced a maximum pulse energy of 13.5 μJ with a pulse width of 130.2 ns at a pulse repetition frequency of 20 kHz, translating to a peak power of around 103.7 W. The Pr3+:YLF MOPA laser system’s output wavelength was 639.7 nm, and the line-width of its laser spectra was roughly 168 MHz. Additionally, at the highest output level, the laser beam quality did not decrease much due to amplification.