bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Class IIa-Selective Histone Deacetylase Inhibitors Discovered Using an in Silico Virtual Screening Approach
Authors: Hsu Kai-Cheng , Liu Chang-Yi , Lin Tony Eight , Hsieh Jui-Hua , Sung Tzu-Ying , Tseng Hui-Ju , Yang Jinn-Moon , Huang Wei-Jan
Published: June 2017

Histone deacetylases (HDAC) contain eighteen isoforms that can be divided into four classes. Of these isoform enzymes, class IIa (containing HDAC4, 5, 7 and 9) target unique substrates, some of which are client proteins associated with epigenetic control. Class IIa HDACs are reportedly associated with some neuronal disorders, making HDACs therapeutic targets for treating neurodegenerative diseases. Additionally, some reported HDAC inhibitors contain hydroxamate moiety that chelates with zinc ion to become the cofactor of HDAC enzymes. However, the hydroxamate functional group is shown to cause undesirable effects and has poor pharmacokinetic profile. This study used in silico virtual screening methodology to identify several nonhydroxamate compounds, obtained from National Cancer Institute database, which potentially inhibited HDAC4. Comparisons of the enzyme inhibitory activity against a panel of HDAC isoforms revealed these compounds had strong inhibitory activity against class IIa HDACs, but weak inhibitory activity against class I HDACs. Further analysis revealed that a single residue affects the cavity size between class I and class IIa HDACs, thus contributing to the selectivity of HDAC inhibitors discovered in this study. The discovery of these inhibitors presents the possibility of developing new therapeutic treatments that can circumvent the problems seen in traditional hydroxamate-based drugs.
QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
Authors: Bhadauria Vijai , Ramsay Larissa , Bett Kirstin E. , Banniza Sabine
Published: June 2017

Lens ervoides, a wild relative of lentil is an important source of allelic diversity for enhancing the genetic resistance of the cultivated species against economically important fungal diseases, such as anthracnose and Stemphylium blight caused by Colletotrichum lentis and Stemphylium botryosum, respectively. To unravel the genetic control underlying resistance to these fungal diseases, a recombinant inbred line (RIL) population (n = 94, F9) originating from a cross between two L. ervoides accessions, L01-827A and IG 72815, was genotyped on the Illumina HiSeq 2500 platform. A total of 289.07 million 100 bp paired-end reads were generated, giving an average 7.53-fold genomic coverage to the RILs and identifying 2,180 high-quality SNPs that assembled in 543 unique haplotypes. Seven linkage groups were resolved among haplotypes, equal to the haploid chromosome number in L. ervoides. The genetic map spanned a cumulative distance of 740.94 cM. Composite interval mapping revealed five QTLs with a significant association with resistance to C. lentis race 0, six QTLs for C. lentis race 1 resistance, and three QTLs for S. botryosum resistance. Taken together, the data obtained in the study reveal that the expression of resistance to fungal diseases in L. ervoides is a result of rearrangement of resistant alleles contributed by both parental accessions.
Development of a selective agonist for relaxin family peptide receptor 3
Authors: Wei Dian , Hu Meng-Jun , Shao Xiao-Xia , Wang Jia-Hui , Nie Wei-Han , Liu Ya-Li , Xu Zeng-Guang , Guo Zhan-Yun
Published: June 2017

Relaxin family peptides perform a variety of biological functions by activating four G protein-coupled receptors, namely RXFP1–4. Among these receptors, RXFP3 lacks a specific natural or synthetic agonist at present. A previously designed chimeric R3/I5 peptide, consisting of the B-chain of relaxin-3 and the A-chain of INSL5, displays equal activity towards the homologous RXFP3 and RXFP4. To increase its selectivity towards RXFP3, in the present study we conducted extensive mutagenesis around the B-chain C-terminal region of R3/I5. Decreasing or increasing the peptide length around the B23–B25 position dramatically lowered the activation potency of R3/I5 towards both RXFP3 and RXFP4. Substitution of B23Gly with Ala or Ser converted R3/I5 from an efficient agonist to a strong antagonist for RXFP3, but the mutants retained considerable activation potency towards RXFP4. Substitution of B24Gly increased the selectivity of R3/I5 towards RXFP3 over the homologous RXFP4. The best mutant, [G(B24)S]R3/I5, displayed 20-fold higher activation potency towards RXFP3 than towards RXFP4, meanwhile retained full activation potency at RXFP3. Thus, [G(B24)S]R3/I5 is the best RXFP3-selective agonist known to date. It is a valuable tool for investigating the physiological functions of RXFP3, and also a suitable template for developing RXFP3-specific agonists in future.
Living microorganisms change the information (Shannon) content of a geophysical system
Authors: Tang Fiona H. M. , Maggi Federico
Published: June 2017

The detection of microbial colonization in geophysical systems is becoming of interest in various disciplines of Earth and planetary sciences, including microbial ecology, biogeochemistry, geomicrobiology, and astrobiology. Microorganisms are often observed to colonize mineral surfaces, modify the reactivity of minerals either through the attachment of their own biomass or the glueing of mineral particles with their mucilaginous metabolites, and alter both the physical and chemical components of a geophysical system. Here, we hypothesise that microorganisms engineer their habitat, causing a substantial change to the information content embedded in geophysical measures (e.g., particle size and space-filling capacity). After proving this hypothesis, we introduce and test a systematic method that exploits this change in information content to detect microbial colonization in geophysical systems. Effectiveness and robustness of this method are tested using a mineral sediment suspension as a model geophysical system; tests are carried out against 105 experiments conducted with different suspension types (i.e., pure mineral and microbially-colonized) subject to different abiotic conditions, including various nutrient and mineral concentrations, and different background entropy production rates. Results reveal that this method can systematically detect microbial colonization with less than 10% error in geophysical systems with low-entropy background production rate.
Identification of novel dysregulated key genes in Breast cancer through high throughput ChIP-Seq data analysis
Authors: Raj Utkarsh , Aier Imlimaong , Semwal Rahul , Varadwaj Pritish Kumar
Published: June 2017

Breast cancer is the most common cancer in women both in the developed and less developed countries, and it imposes a considerable threat to human health. Therefore, in order to develop effective targeted therapies against Breast cancer, a deep understanding of its underlying molecular mechanisms is required. The application of deep transcriptional sequencing has been found to be reported to provide an efficient genomic assay to delve into the insights of the diseases and may prove to be useful in the study of Breast cancer. In this study, ChIP-Seq data for normal samples and Breast cancer were compared, and differential peaks identified, based upon fold enrichment (with P-values obtained via t-tests). The Protein–protein interaction (PPI) network analysis was carried out, following which the highly connected genes were screened and studied, and the most promising ones were selected. Biological pathway involved in the process were then identified. Our findings regarding potential Breast cancer-related genes enhances the understanding of the disease and provides prognostic information in addition to standard tumor prognostic factors for future research.
Morphology controls how hippocampal CA1 pyramidal neuron responds to uniform electric fields: a biophysical modeling study
Authors: Yi Guo-Sheng , Wang Jiang , Deng Bin , Wei Xi-Le
Published: June 2017

Responses of different neurons to electric field (EF) are highly variable, which depends on intrinsic properties of cell type. Here we use multi-compartmental biophysical models to investigate how morphologic features affect EF-induced responses in hippocampal CA1 pyramidal neurons. We find that the basic morphologies of neuronal elements, including diameter, length, bend, branch, and axon terminals, are all correlated with somatic depolarization through altering the current sources or sinks created by applied field. Varying them alters the EF threshold for triggering action potentials (APs), and then determines cell sensitivity to suprathreshold field. Introducing excitatory postsynaptic potential increases cell excitability and reduces morphology-dependent EF firing threshold. It is also shown that applying identical subthreshold EF results in distinct polarizations on cell membrane with different realistic morphologies. These findings shed light on the crucial role of morphologies in determining field-induced neural response from the point of view of biophysical models. The predictions are conducive to better understanding the variability in modulatory effects of EF stimulation at the cellular level, which could also aid the interpretations of how applied fields activate central nervous system neurons and affect relevant circuits.
Zinc finger gene 217 (ZNF217) Promoted Ovarian Hyperstimulation Syndrome (OHSS) through Regulating E2 Synthesis and Inhibiting Thrombospondin-1 (TSP-1)
Authors: Zhai Junyu , Liu Jiansheng , Cheng Xiaoyue , Li Shang , Hong Yan , Sun Kang , Chen Zi-Jiang , Du Yanzhi , Li Weiping
Published: June 2017

Zinc finger gene 217 (ZNF217) is a candidate gene of polycystic ovary syndrome (PCOS) which is vulnerable to ovarian hyperstimulation syndrome (OHSS). However, the relationship between ZNF217 and OHSS is largely unknown. Our study demonstrated that ZNF217 was mainly distributed in the granulosa cells of rat ovary. Significantly higher expression of ovarian ZNF217 was detected in OHSS rats, being consistent with serum 17β-estradiol concentration and ovarian aromatase. Moreover, OHSS rats also showed decreased ovarian TSP-1 mRNA, an acknowledged VEGF signaling suppressor. The same changes were detected in human granulosa cells and follicular fluid. Thus, the increased ZNF217 and decreased TSP-1 may participate in OHSS onset. In vitro experiment revealed that ZNF217 positively regulated E2 synthesis through promoting cAMP response element binding protein (CREB) and thereby CYP19A1 in KGN cells. Furthermore, ZNF217 negatively regulated TSP-1 in KGN cells while TSP-1 promoted claudin1 and inhibited nitric oxide (NO) in HUVECs and HAECs. Both of claudin1 and NO are responsible for the regulation of vascular permeability (VP). Therefore, we demonstrated that ZNF217 contributed to OHSS onset through promoting E2 synthesis and the increase of VP. Moreover, the increased ZNF217 and decreased TSP-1 provided new targets for the prevention or treatment of OHSS in the future.
Construction of SnO2−Graphene Composite with Half-Supported Cluster Structure as Anode toward Superior Lithium Storage Properties
Authors: Zhu Chengling , Chen Zhixin , Zhu Shenmin , Li Yao , Pan Hui , Meng Xin , Imtiaz Muhammad , Zhang Di
Published: June 2017

Inspired by nature, herein we designed a novel construction of SnO2 anodes with an extremely high lithium storage performance. By utilizing small sheets of graphene oxide, the partitioned-pomegranate-like structure was constructed (SnO2@C@half-rGO), in which the porous clusters of SnO2 nanoparticles are partially supported by reduced graphene oxide sheets while the rest part is exposed (half-supported), like partitioned pomegranates. When served as anode for lithium-ion batteries, SnO2@C@half-rGO exhibited considerably high specific capacity (1034.5 mAh g−1 after 200 cycles at 100 mA g−1), superior rate performance and remarkable durability (370.3 mAh g−1 after 10000 cycles at 5 A g−1). When coupled with graphitized porous carbon cathode for lithium-ion hybrid capacitors, the fabricated devices delivered a high energy density of 257 Wh kg−1 at ∼200 W kg−1 and maintained 79 Wh kg−1 at a super-high power density of ∼20 kW kg−1 within a wide voltage window up to 4 V. This facile and scalable approach demonstrates a new architecture for graphene-based composite for practical use in energy storage with high performance.
Methylation of DACT2 promotes breast cancer development by activating Wnt signaling
Authors: Li Jingyi , Zhang Meiying , He Tao , Li Hongxia , Cao Tingting , Zheng Lili , Guo Mingzhou
Published: June 2017

Breast cancer is the most common malignant tumor in women worldwide. To explore the role of DACT2 in breast cancer, 5 cell lines and 153 cases of primary cancer were studied. The expression of DACT2 was detected in BT474, MDA-MB-231 and BT549 cells, while no expression was found in MDA-MB-468 and HBL100 cells. Complete methylation of DACT2 was found in MDA-MB-468 and HBL100 cells, partial methylation was observed in BT474 and BT549 cells, and no methylation was detected in MDA-MB-231 cells. Restoration of DACT2 expression was induced by 5-Aza in MDA-MB-468 and HBL100 cells. DACT2 was methylated in 49.7% (76/153) of primary breast cancer samples. Methylation of DACT2 was significantly associated with tumor size (P < 0.05). Reduced DACT2 expression was significantly associated with promoter region methylation in primary breast cancer (P < 0.05). DACT2 suppressed breast cancer cell growth and induced G1/S phase arrest in breast cancer cells. DACT2 inhibited Wnt/β-catenin signaling in human breast cancer cells and suppressed breast cancer cell tumor growth in xenograft mice. In conclusion, our results demonstrate that DACT2 is frequently methylated in human breast cancer, methylation of DACT2 activates Wnt signaling, and DACT2 suppresses breast cancer cell growth both in vitro and in vivo.
GABAA receptor subunit gene polymorphisms predict symptom-based and developmental deficits in Chinese Han children and adolescents with autistic spectrum disorders
Authors: Yang Shuhan , Guo Xuan , Dong Xiaopeng , Han Yu , Gao Lei , Su Yuanyuan , Dai Wei , Zhang Xin
Published: June 2017

GABAA receptor subunit genes GABRB3, GABRA5, and GABRG3 located on chromosome 15q11-q13 have been implicated in the etiology of autistic spectrum disorders (ASD). This study intended to investigate the possible role of single-nucleotide polymorphisms (SNPs) present in GABRB3 (rs2081648 and rs1426217), GABRA5 (rs35586628), and GABRG3 (rs208129) genes in ASD susceptibility and symptom-based and developmental phenotypes of ASD in Chinese Han children and adolescents. 99 ASD patients and 231 age- and gender- frequency-matched typical developing (TD) controls were tested by TaqMan® genotyping assay. Symptom-based phenotypes were evaluated by Childhood Autism Rating Scale (CARS) and Autism Behavior Checklist (ABC), and developmental phenotypes were assessed by Early Childhood Development Questionnaire (ECDQ) in ASD patients. Three haplotypes and global χ
2
test of all SNPs demonstrated significant associations between ASD and TD groups. Besides, GABRB3 rs2081648, GABRA5 rs35586628, and GABRG3 rs208129 polymorphisms were associated with symptom-based deficits in social interaction, sensorimotor and somatosensory coordination, visual response, imitation, activity level, language expression and adaptability. Developmental abnormalities in late emergences of social interaction and fine motor were detected in GABRB3 rs2081648 polymorphism. Overall results indicated that gene synergy may participate in ASD pathogenesis, and GABAA receptor gene polymorphisms can predict symptom-based and developmental deficits in ASD individuals.
Unifying the microscopic picture of His-containing turns: from gas phase model peptides to crystallized proteins
Authors: Sohn Woon Yong , Habka Sana , Gloaguen Eric , Mons Michel
Published: June 2017

The presence in crystallized proteins of a local anchoring between the side chain of a His residue, located in the central position of a γ- or β-turn, and its local main chain environment, is assessed by the comparison of protein structures with relevant isolated model peptides.
Tailoring the local environment around metal ions: a solution chemical and structural study of some multidentate tripodal ligands
Authors: Matyuska Ferenc , Szorcsik Attila , May Nóra V. , Dancs Ágnes , Kováts Éva , Bényei Attila , Gajda Tamás
Published: June 2017

The four polydentate tripodal ligands studied here exhibit a high thermodynamic stability and versatile coordination geometry for manganese(ii), copper(ii) and zinc(ii).
Thiocyanate-free ruthenium(ii) sensitizers with a bi-imidazole ligand in dye-sensitized solar cells (DSSCs)
Authors: Ashraf Saba , Akhtar Javeed , Siddiqi Humaira M. , El-Shafei Ahmed
Published: June 2017

In this study, tris-heteroleptic Ru(ii) sensitizers denoted as SD-15 and SD-16 with a 4,4′,5,5′-tetramethyl-1H,1′H-2,2′-bis-imidazole moiety as the ancillary ligand were designed, synthesized and characterized.
Tuning the Schottky contacts at the graphene/WS2 interface by electric field
Authors: Zhang Fang , Li Wei , Ma Yaqiang , Tang Yanan , Dai Xianqi
Published: June 2017

Evolution of Schottky barriers of ΦBp and ΦBn in graphene/WS2 heterostructures as a function of external electric field.
The chain order of binary unsaturated lipid bilayers modulated by aromatic-residue-containing peptides: an ATR-FTIR spectroscopy study
Authors: Peng Bo , Ding Xiao-Yan , Sun Chao , Yang Ya-Nan , Gao Yu-Jiao , Zhao Xin
Published: June 2017

It highlights the importance of aromatic residues in influencing peptide binding to the membrane, demonstrates that the stability of the membranes depends on the lipid composition and the sequence, structural context, and orientation of the peptides.
Low-temperature dynamic vacuum annealing of ZnO thin film for improved inverted polymer solar cells
Authors: Ma Zhu , Lu Honglin , Zhao Fenggui , Xiang Yan , Zhuang Jia , Li Haimin
Published: June 2017

Low temperature dynamic vacuum annealing was developed for preparing ZnO cathode buffer layer to improve the performance of IPSCs.
F127DA micelle cross-linked PAACA hydrogels with highly stretchable, puncture resistant and self-healing properties
Authors: Liu Yunfei , Zhou Wanfu , Zhou Quan , Peng Kang , Yasin Akram , Yang Haiyang
Published: June 2017

F127DA micelle cross-linked PAACA hydrogels with highly stretchable, puncture resistant and self-healing properties are prepared.
High-performance thermal sensitive VO2(B) thin films prepared by sputtering with TiO2(A) buffer layer and first-principles calculations study
Authors: Ding Zhuohan , Cui Yuanyuan , Wan Dongyun , Luo Hongjie , Gao Yanfeng
Published: June 2017

We present an effective strategy to modify the electronic properties of VO2(B) by inducing elastic strain with TiO2(A) buffer layer.
Fabrication of core–shell Ag@pDA@HAp nanoparticles with the ability for controlled release of Ag+and superior hemocompatibility
Authors: Chen Keling , Xie Kenan , Long Qin , Deng Lijun , Fu Zhiqiang , Xiao Huanhuan , Xie Lu
Published: June 2017

We report a convenient and effective method to prepare Ag-NPs and core–shell Ag@pDA@HAp-NPs.
Dual temperature- and pH-responsive polymeric micelle for selective and efficient two-step doxorubicin delivery
Authors: Hiruta Yuki , Kanda Yuki , Katsuyama Naoya , Kanazawa Hideko
Published: June 2017

Dual responsive polymeric micelle enabled selective intracellular uptake with thermal stimulation and effective release of doxorubicin at acidic endosomal pH.
Isolation and phototransformation of enantiomerically pure iridium(iii) bis[(4,6-difluorophenyl)pyridinato-N,C2]picolinate
Authors: Wang Yue , Harada Takunori , Shiota Yoshihito , Yoshizawa Kazunari , Wang Heng , Wang Sheng , Ye Xichong , Ogasawara Masamichi , Nakano Tamaki
Published: June 2017

Here we report the resolution of phosphorescent light-emitting iridium(iii) bis[(4,6-difluorophenyl)pyridinato-N,C2]-picolinate into its respective enantiomers by using chiral HPLC and the photo-induced transformation of the isolated enantiomers.
Lewis base-catalyzed diastereoselective [3 + 2] cycloaddition reaction of nitrones with electron-deficient alkenes: an access to isoxazolidine derivatives
Authors: Liu Honglei , Zhao Yan , Li Zhen , Jia Hao , Zhang Cheng , Xiao Yumei , Guo Hongchao
Published: June 2017

A Lewis base-catalyzed [3 + 2] cycloaddition reaction of nitrones with electron-deficient alkenes has been achieved under mild reaction conditions, affording functionalized isoxazolidines as single diastereomers in moderate to excellent yields.
Synthesis and electrochemical performance of NaV6O15 microflowers for lithium and sodium ion batteries
Authors: Hu Fang , Jiang Wei , Dong Yidi , Lai Xiaoyong , Xiao Li , Wu Xiang
Published: June 2017

High first discharge capacity of 255 mA h g−1 (vs. Li+/Li) and 130 mA h g−1 (vs. Na+/Na) were observed in NaV6O15 microflowers and the capacity retention reaches 105% and 64% after 50 cycles at the current density of 100 mA g−1 and 50 mA g−1, respectively.
Phenolics profiling of pomace extracts from different grape varieties cultivated in Argentina
Authors: Fontana Ariel , Antoniolli Andrea , D'Amario Fernández María Agustina , Bottini Rubén
Published: June 2017

Grape pomace can be considered as an excellent and inexpensive source of phenolic compounds with potential bioactive properties.
High-temperature solid-state reaction induced structure modifications and associated photoactivity and gas-sensing performance of binary oxide one-dimensional composite system
Authors: Liang Yuan-Chang , Lo Ya-Ju
Published: June 2017

The effects of high-temperature solid-state reactions on the microstructures, optical properties, photoactivity, and low-concentration NO2 gas-sensing sensitivity of ZnO–SnO2 core–shell nanorods were investigated.
Neutral linear supramolecular polymers constructed by three different interactions
Authors: Wang Qi , Zhang Peng , Li Yuanyuan , Tian Lu , Cheng Ming , Lu Feng , Lu Xiaomei , Fan Quli , Huang Wei
Published: June 2017

Neutral linear supramolecular polymers were constructed by the combination of quadruple hydrogen bonding, pillar[5]arene-based molecular recognition and π–π donor–acceptor interactions.
PtFe nanoparticles supported on electroactive Au–PANI core@shell nanoparticles for high performance bifunctional electrocatalysis
Authors: Lee Ji-Eun , Jang Yu Jin , Xu Wenqian , Feng Zhenxing , Park Hee-Young , Kim Jin Young , Kim Dong Ha
Published: June 2017

Efficient bifunctional electrocatalytic activity for reduction and oxidation reactions based on the AuNP@PANI@PtFe core–multishell nanostructures is presented. The AuNP@PANI@PtFe exhibits an enhanced catalytic activity and durability for ORR and MOR than conventional carbon supported Pt catalysts.
Palladium dendron encapsulated nanoparticles grown from MCM-41 and SBA-15
Authors: Lou Y. , Shantz D. F.
Published: June 2017

The synthesis and characterization of palladium nanoparticles templated in melamine-based dendrons grown from ordered mesoporous silica (OMS) are reported.
Simulations of non-monolithic tandem solar cell configurations for electrolytic fuel generation
Authors: White R. Turner , Kumar Bijandra , Kumari Sudesh , Spurgeon Joshua M.
Published: June 2017

Multi-terminal tandem solar cell configurations were modeled for driving water-splitting and CO2 reduction to maximize fuel production throughout the day.
Two-photon red-emissive fluorescent probe for imaging nitroxyl (HNO) in living cells and tissues
Authors: Dong Baoli , Song Xuezhen , Kong Xiuqi , Wang Chao , Zhang Nan , Lin Weiying
Published: June 2017

A two-photon red-emissive fluorescent probe has been developed for imaging nitroxyl (HNO) in living cells and tissues.
Noncomprehension Signaling in Males and Females With Fragile X Syndrome
Authors: Thurman Angela John , Kover Sara T. , Ted Brown W. , Harvey Danielle J. , Abbeduto Leonard
Published: June 2017

Purpose

This study used a prospective longitudinal design to evaluate the trajectory and predictors of noncomprehension signaling in male and female youth with fragile X syndrome (FXS).

Method

A direction-following task in which some of the directions were inadequate was administered. Participants were 52 youth (36 boys, 16 girls) with FXS. Upon study entry, participants ranged from 10 to 16 years. The average number of annual assessments per participant was 3.65 (range = 1–4), providing 198 data points for analysis.

Results

Participants with FXS were less likely to signal noncomprehension than younger, typically developing, cognitively matched children. The average rate of change in noncomprehension signaling was not significantly different from 0 for either boys or girls, suggesting a plateau. Both FMRP and nonverbal IQ were significant independent predictors of noncomprehension signaling for boys. Variability in noncomprehension signaling among girls was not explained by any of the predictors, but trends similar to those observed for boys were observed.

Conclusions

Noncomprehension signaling appears to be an area of weakness for individuals with FXS. Because the failure to signal noncomprehension can have negative, cumulative effects on comprehension, the results suggest a need for interventions targeting the requisite cognitive skills.
A Case of Pituitary Carcinoma Initially Diagnosed as an Ectopic Growth Hormone Producing Pituitary Adenoma with a High Ki-67 Labeling Index
Authors: Endo Toshiki , Watanabe Mika , Tominaga Teiji , Ogawa Yoshikazu
Published: June 2017

We report a case of a 66-year-old woman with a malignant transformation of a growth hormone (GH)-producing pituitary adenoma that was mainly located in the clivus. The patient originally presented with left oculomotor and abducens nerve palsies. Magnetic resonance imaging revealed a clival tumor separated from the normal pituitary gland, and the patient subsequently underwent transsphenoidal surgery. Analysis of the resected clival tissue revealed proliferation of tumor cells with slightly irregular nuclei but no mitosis. Although the Ki-67 labeling index was as high as 8.7%, p53 was negative. Histologic analysis confirmed the diagnosis of a GH-producing pituitary adenoma. Two months after the first operation, the tumor acutely enlarged and caused a subarachnoid hemorrhage. Pathologic findings of the second surgical specimen showed significant nuclear atypia. The Ki-67 labeling index increased to 27.7% and the p53 was positive; there was no GH immunoreactivity. Following the second surgical intervention, the patient was diagnosed with an atypical pituitary adenoma and underwent postoperative local radiotherapy (50 Gy in 25 fractions). Twenty months after the first surgery, multiple bone metastases were detected that led to the diagnosis of pituitary carcinoma. This is the first report of a clival pituitary adenoma undergoing malignant transformation. Because neuroimaging may underestimate local invasiveness in ectopic pituitary tumors, it is essential to conduct a histologic examination to evaluate it. Further analysis of similar cases is necessary to improve clinical management for this confused diagnostic criterion.
Completely Thrombosed Aneurysms of the Posterior Cerebral Artery: A Comprehensive Review
Authors: Cuartero Pérez Beatriz , Ros de San Pedro Javier
Published: June 2017

Background Completely thrombosed aneurysms of the posterior cerebral artery (CTPCAAs) are rare lesions with different characteristics and clinical behavior when compared with other intracranial aneurysms.

Objective To describe and analyze the main features of CTPCAAs.

Material and Methods Fifteen CTPCAAs were studied (14 literature cases plus one illustrative case). Clinical, radiologic, pathologic, and therapeutic data were obtained and analyzed.

Results CTPCAAs are large (mean: 2.43 cm), located proximally (80% at P1–P2 segments), and show a slight predominance of a fusiform appearance. Posterior cerebral artery (PCA) occlusion ensued after aneurysm complete thrombosis in 73% of patients (11/15). However, only 33% of patients developed a PCA territory stroke, due to a rich distal arterial collateral network. Three mutually exclusive clinical presentations were observed: subarachnoid hemorrhage (SAH) (n = 3), “stroke-like” syndrome (n = 7), and “tumor-like” syndrome (n = 4). One case was incidental. Three treatments were performed: conservative (n = 7), endovascular (n = 1), and surgery (n = 7). CTPCAA exclusion was the goal of surgery. Neural structures decompression was also sought in tumor-like cases. CTPCAA elimination as a potential bleeding source was the target in stroke-like cases. General outcome among CTPCAAs was good (73% of cases scored Glasgow Outcome Scale 4–5/Modified Rankin Scale 0–2).

Conclusions CTPCAAs are characterized by young age at presentation, male predominance, proximal location on PCA, and tendency to cause PCA occlusion. The PCAA clinical presentation depends on the mechanism of complete thrombosis. SAH and stroke-like syndrome CTPCAAs harbor a less organized thrombus than tumor-like CTPCAAs, which makes the aneurysm more prone to hemorrhagic/ischemic complications. Thus clinical presentation may indicate the clinical evolution in CTPCAAs. The management of CTPCAAs varied according to the patient's clinical condition and the risk evaluation for future complications derived from the aneurysm. Clinical surveillance and surgical removal were the most frequent treatment options performed. Surgery was focused on neural decompression and/or CTPCAA elimination as a potential source of bleeding.
Elternschaft, Partnerschaft und familiäre Konstellationen bei Methamphetaminkonsumierenden – Eine Analyse der Beratungsdokumentation in der ambulanten Suchthilfe
Authors: Dyba Janina , Klein Michael , Wetzel Wolfgang
Published: June 2017

Ziel der Studie Die Studie beschreibt die familiäre Situation Methamphetaminkonsumierender in der ambulanten Suchtberatung mit speziellem Fokus auf Konstellationen von Elternschaft und Partnerschaft.

Methoden Es wurde ein Dokumentenanalysebogen entwickelt, anhand dessen die Fallakten der Jahre 2013 und 2014 von Methamphetamin konsumierenden Eltern in der ambulanten Suchtberatungsstelle in Zwickau anonymisiert analysiert wurden. Fokussiert wurden dabei neben soziodemografischen und konsumbezogenen Angaben, besonders Informationen zu Partnerschaft und Kindern.

Ergebnisse In den Familien finden sich häufig junge Kinder, welche in der Regel (zumindest zeitweise) nicht beim konsumierenden Elternteil aufwachsen. Partnerschaften zeigen sich fragil und geprägt vom Substanzgebrauch beider Partner.

Schlussfolgerung Die Familiensysteme offenbaren multiple Problemlagen und sind vielmals geprägt von Diskontinuität und Instabilität, wobei sich erhebliche Entwicklungsrisiken für die Kinder abzeichnen.
Editorial
Authors: Klein Michael
Published: June 2017

Das vorliegende Heft thematisiert erstmals in Deutschland in der wissenschaftlichen Zeitschrift umfassend die Situation suchtkranker Eltern. Nachdem bereits in den Themenheften 3/2001 Kinder von Suchtkranken, 2/2006 Sucht und Familie sowie 4/2011 Schwangerschaft und Sucht im Fokus standen, ist die Betrachtung der Elternschaft im Kontext von Sucht folgerichtig und notwendig. Auf der Basis vorliegender epidemiologischer Studien ist bei ca. 3,4 Millionen Menschen mit einer Alkoholstörung und ca. 400000 mit einer Drogenstörung von mehr als 2 Millionen Personen auszugehen, die in einer Elternverantwortung standen oder aktuell stehen. Welche Rollenmodelle sind sie für ihre Kinder? Wie sind ihre Erziehungspraktiken? Welche Verhaltensweisen zeigen sie gegenüber ihren Kindern unter Substanzeinfluss? Manche Antworten zu diesen Fragen können heute schon gegeben werden. Viele Fragen müssen heute immer noch als nicht zu beantworten gelten, wie die Recherchen zu diesem Themenheft gezeigt haben.
Osteopathische Behandlungen für das neurovegetative
System
Authors: Vogt Ralf
Published: June 2017

Das vegetative Nervensystem stellt als übergeordnetes System der Körpersteuerung
eine besondere Herausforderung für die osteopathische Behandlung dar. Es sprengt
den Rahmen der üblichen Betrachtungsweise, bei der oft in parietal, viszeral und
kraniosakral unterteilt wird. Wir können den Körper nur in seiner Ganzheit
behandeln, indem wir versuchen, seine neurovegetative Autoregulation über
Strukturen des Nervensystems, des endokrinen Systems und der alles begleitenden
Fasziensysteme zu aktivieren. Dies erfordert auch bei den verwendeten Techniken
einen übergreifenden Ansatz.
Leave it alone, and then ...?
Authors: Stark Jane
Published: June 2017

Finde die Läsion, passe sie an und überlasse den Rest der Physiologie des Körpers
– so könnte man den bekannten, Andrew Taylor Still zugeschriebenen Leitspruch
„Find it, fix it and leave it alone“ der Osteopathie ins Deutsche übersetzen.
Doch stammt diese Aussage tatsächlich von Still, und wie zeitgemäß ist sie?
Moderne Interpretationen zeigen: Im Zentrum von Stills Aussage standen das
physiologische System des Menschen und die Selbstorganisationskräfte der
Natur.
Knieschmerz aus viszeralosteopathischer Sicht
Authors: Bonacker Michael
Published: June 2017

Ein 14-jähriges Mädchen kommt wegen Knieschmerzen zu mir in die Praxis. Interessanterweise lösten bisherige orthopädische und
physiotherapeutische Maßnahmen nicht ansatzweise ihr Problem. Dies hatte aber für mich den Vorteil, dass ich möglicherweise eine parietale
Kausalität ausschließen konnte. Eine ausführliche Anamnese und körperliche Untersuchung in Verbindung mit dem Background der neuralen
Vernetzung der Becken-Bauch-Region zur unteren Extremität brachten mich auf eine außergewöhnliche Idee.
Keratin, Kollagen oder doch Spendergewebe – wo liegt die Zukunft in der Entwicklung neuer Biomaterialien zur Hornhautrekonstruktion?
Authors: Bachmann Björn , Schrader Stefan
Published: June 2017

Für den Ersatz von Hornhautgewebe werden seit Langem standardmäßig Hornhauttransplantate bzw. Amnionmembran verwendet. Da es sich hierbei um biologisches Gewebe handelt, besteht nur eine eingeschränkte Standardisierung, was die Herstellung, Beschaffenheit und die Eigenschaften nach Transplantation betrifft. Darüber hinaus gibt es ein Risiko der Krankheitsübertragung vom Spender und die Verfügbarkeit sowohl von menschlichen Hornhäuten als auch von Amnionmembran ist in vielen Regionen der Erde ungenügend. Aus diesem Grund werden seit vielen Jahren alternative Biomaterialien für den Hornhautersatz beforscht. Unter den natürlichen Biomaterialien bieten Materialien auf Kollagen- oder Keratinbasis Eigenschaften, die sie zu aussichtsreichen Kandidaten für den Hornhautstromaersatz machen. Aktuell bestehen aber noch viele ungelöste Probleme, insbesondere was die Degradation nach Implantation und die Nahtfestigkeit der Materialien angeht. Erste klinische Untersuchungen mit unterschiedlichen Biomaterialien auf Kollagenbasis belegen jedoch ihre insgesamt recht gute Biokompatibilität hinsichtlich ihrer Integrationsfähigkeit bzw. hinsichtlich ihrer geringen Immunogenität. Aktuell gibt es kein Biomaterial, das den Anforderungen in jeder Situation gerecht wird. Es ist zu vermuten, dass zukünftig unterschiedliche Biomaterialen zur Verfügung stehen, die in Abhängigkeit von der zugrunde liegenden Hornhauterkrankung unterschiedliche Funktionen erfüllen und so eine patienten- und krankheitsindividuelle Versorgung möglich machen.
Erstellung eines Handlungsleitfadens für einen gesundheitsförderlichen Umgang mit Informations- und Kommunikationstechnologien, Flexibilisierung und Erreichbarkeit in kleinen und mittleren Unternehmen
Authors: Weilnhammer Veronika , Heinze Stefanie , Heiden Barbara , Palm Esther , Herbig Britta , Lüke Gabriele , Nowak Dennis , Glaser Jürgen , Herr Caroline
Published: June 2017

Hintergrund Innerhalb der letzten Jahrzehnte hat sich die Arbeitswelt stark gewandelt. Neue Informations- und Kommunikationstechnologien (IKT) machen es vielfach möglich, zu jeder Zeit an jedem Ort zu arbeiten. Flexibilisierung, erweiterte Erreichbarkeit und Entgrenzung von Arbeit und Privatleben können möglicherweise daraus resultieren. Die Verbreitung von IKT und mögliche Auswirkungen dieser Entwicklung auf die Gesundheit und das Wohlbefinden betroffener Mitarbeiter wurden bisher v. a. in großen Unternehmen untersucht. Kleine und mittlere Unternehmen (KMU) sind in diesem Zusammenhang selten Gegenstand wissenschaftlicher Studien.

Ziel Ziel dieser Arbeit war es daher, anhand der Erfahrungen und Ergebnissen einer Studie zu Flexibilisierung in der Arbeitswelt (FlexA) sowie einer systematischen Literaturrecherche einen Handlungsleitfaden (HLF) zu entwickeln. Dieser richtet sich an KMU mit dem Ziel, einen gesundheitsförderlichen Umgang mit IKT zu erarbeiten und in die Unternehmenskultur zu integrieren.

Methodik Um den Stand der Forschung zu IKT-bezogenen Interventionen abzubilden, wurde eine systematische Literaturrecherche in verschiedenen Datenbanken, z. B. PsycINFO, EconLit und Medline durchgeführt. Dazu wurden u. a. die Begriffe „Arbeit“, „Intervention“, „IKT“ verwendet und in eine Suchstrategie überführt. Zusätzlich wurden graue Literatur und die Ergebnisse des FlexA Projektes zur Erstellung des HLF einbezogen.

Ergebnisse Von den 30 Treffern der systematischen Literaturrecherche konnte, nach Screening von Titel und Abstract anhand festgelegter Ausschlusskriterien, keine Arbeit eingeschlossen werden. Aus grauer Literatur konnten 4 Veröffentlichungen als relevant identifiziert werden. Die in den Unternehmensworkshops des FlexA Projektes erarbeiteten Maßnahmen, wie z. B. das Einführen von kollektiven Regelungen in den Betrieben, wurden in den HLF aufgenommen.

Schlussfolgerung Aufgrund der permanenten Weiterentwicklung von IKT und den damit verbundenen Veränderungen in Bezug auf die Flexibilisierung in der Arbeitswelt, wird es immer wichtiger werden, diese Entwicklung zu begleiten, aktiv und gesundheitsförderlich zu gestalten und innerhalb des jeweiligen Unternehmens in geregelten Bahnen stattfinden zulassen. Der vorliegende Leitfaden soll Unternehmer bei dieser Aufgabe unterstützen und ist ein wichtiges Instrument für den Umgang mit IKT hinsichtlich Flexibilisierung und Erreichbarkeit im Arbeitsalltag.
Zum Stand der Keratoprothesen mit biologischer Haptik – Die Düsseldorf-Offenburger-Erfahrungen mit Osteo-Odonto- und Tibia-Keratoprothese
Authors: Geerling Gerd , Hille Konrad
Published: June 2017

Bei schweren Erkrankungen der Augenoberfläche mit limbaler Stammzellinsuffizienz, bei denen eine perforierende Keratoplastik, z. B. bei schwerem Sicca-Syndrom, nicht erfolgreich durchgeführt werden kann, lässt sich bei intakten hinteren Augenabschnitten durch die Implantation einer Keratoprothese (KPro) eine Visusrehabilitation erreichen. Bei einer KPro mit biologischer Haptik wird der Prothesenzylinder entweder über ein autologes Zahn-Knochen-Präparat (Osteo-Odonto-Keratoprothese, OOKPro) oder über den kortikalen Knochen der Tibia des Patienten (Tibia-Keratoprothese, TKPro) fest in der Körperoberfläche integriert. Aufgrund dieser anatomischen Integration kann auch bei Patienten mit einem schweren Sicca-Syndrom und autoimmunologischen Erkrankungen, bei denen andere Prothesendesigns häufig versagen, erfolgreich eine KPro implantiert werden. Wir berichten über die anatomischen Langzeitergebnisse von OOKPros und TKPros in der Literatur und bei unseren eigenen Patienten. Bei einer mittleren Nachbeobachtungszeit von 7,5 Jahren (max. 20,6) bei der OOKPro und 4,5 Jahren (max. 13,3) bei der TKPro bestand eine Retentionsrate von 91 bzw. 86%. Uns ist kein anderes Verfahren zum künstlichen Ersatz einer Hornhaut bekannt, das vergleichbar gute Langzeitergebnisse erreicht wie eine KPro mit biologischer Haptik.
Hydrostatic pressure effect on the spin reorientation transition of ferromagnetic Sm0.7−xLaxSr0.3MnO3 (x = 0, 0.1) polycrystals
Authors: Thiyagarajan R. , Arumugam S. , Sivaprakash P. , Kannan M. , Saravanan C. , Yang Wenge
Published: June 2017

The hydrostatic pressure effect on the resistivity and magnetization of the narrow band gap manganite Sm0.7−xLaxSr0.3MnO3 (x = 0, 0.1) systems has been investigated. At ambient pressure measurements, the parent compound Sm0.7Sr0.3MnO3 showed a ferromagnetic-insulating nature, whereas the 10% La-doped compound Sm0.6La0.1Sr0.3MnO3 showed a ferromagnetic-metallic nature. Furthermore, both samples showed a spin-reorientation transition (TSR) below Curie temperature, which originated from the Mn sublattice and was supported by an antiferromagnetic Sm(4f)-Mn(3d) interaction. Both samples exhibited a normal and inverse magnetocaloric effect as a result of these two different magnetic transitions. Magnetization measurements on Sm0.7Sr0.3MnO3 under pressure did not show an appreciable change in the Curie temperature, but enhanced TSR, whereas an insulator-metallic transition was observed during resistivity measurements under pressure. On the other hand, for Sm0.6La0.1Sr0.3MnO3, TC increased and TSR reduced upon the application of pressure. The metallic nature which is observed at ambient pressure resistivity measurement was further enhanced with 97% of piezoresistance. The pressure did not change the normal magnetocaloric effect of Sm0.7Sr0.3MnO3, but increased it in Sm0.6La0.1Sr0.3MnO3. However, there was not much change in the inverse magnetocaloric effect of both compounds. These studies were analyzed based on the pressure effect on the activation energy and scattering interaction factors.
Pregnancy establishment and maintenance after the administration of equine chorionic gonadotropin (eCG) associated or not with gonadotropin-releasing hormone (GnRH) after insemination in sheep
Authors: García-Pintos C. , Menchaca A.
Published: June 2017

It was recently demonstrated that equine chorionic gonadotropin (eCG) treatment 10 days after ovulation increases serum progesterone concentrations in sheep. The objective of the present study was to evaluate pregnancy outcomes induced with the administration of eCG and gonadotropin-releasing hormone (GnRH) after insemination. Three experiments were conducted in multiparous ewes that received fixed-time intrauterine insemination (Day 0) after a progesterone-based protocol for oestrus synchronisation. Experiment 1 consisted of 408 ewes, randomly assigned to three experimental groups; ewes in the first and second group received 400 IU of eCG on Day 5 and Day 10 after insemination respectively, while ewes on the third (control) group did not receive eCG. In Experiment 2, 587 ewes were randomly assigned to four experimental groups (2 × 2 factorial experiment), to receive or not 400 IU of eCG on Day 5, associated or not with 100 µg of GnRH analogue on Day 6 (i.e. 30 h after eCG administration). In Experiment 3, 715 ewes were randomly assigned to four experimental groups (2 × 2 factorial experiment), to receive or not 400 IU of eCG on Day 10, associated or not with 100 µg of GnRH analogue on Day 11 (i.e. 30 h after eCG administration). Pregnancy diagnosis was determined on Day 30 and Day 70 by ultrasonography. The eCG treatment after insemination failed to improve pregnancy outcomes in Experiments 1 and 2. However, in Experiment 3 the treatment with eCG on Day 10 enhanced pregnancy rate on Day 30, although this improvement was not maintained on Day 70, which showed greater pregnancy failure. The administration of GnRH on Day 6 or Day 11, preceded or not by eCG, did not affect any of the variables related to pregnancy. In summary, the improvement in progesterone profiles induced by eCG treatment on Day 10 after insemination, previously reported in sheep, seems to have a marginal positive effect on pregnancy establishment during the first month, which is not maintained during the second month of gestation.
Flooding effects on grassland species composition in the Azul creek basin, Argentina
Authors: Entraigas Ilda , Vercelli Natalia , Ares Guadalupe , Varni Marcelo , Zeme Sofía
Published: June 2017

From a hydrological point of view, the characteristic of the water behaviour in catchments so depressed as the Azul creek basin (centre of Buenos Aires province, Argentina) is water accumulation above the land surface. Thus, water on the ground does not have a single runoff direction, but moves in a disorderly, indefinite and unpredictable way. Considering that periodic floods are a typical disturbance of the region, the objective of this study is to analyse, under field conditions, the transformative effect of prolonged flooding on floristic composition, taking into account the different vegetation patches and their relative position over the relief, the chemical characteristics and the groundwater fluctuation, and some edaphic properties in each site.
Vegetation samplings were performed during three consecutive springs, when the grassland was on different hydrological conditions due to very different rainfall precedent histories. A digital terrain model of the study area was built and a flow accumulation map was created from it. Pits were dug to describe edaphic variables and shallow wells were drilled for monitoring the groundwater characteristics. Flooding, in relation with surface and groundwater dynamics and soil characteristics, is the factor that determines and promotes the differentiation among sites that are relatively close, contiguous and even topographically in almost identical positions. So, some patches of vegetation get their differentiation through the limiting conditions of their soils, while others receive greater influence from the hydrodynamics to which they are subject. Thus, in this study it becomes evident how certain stands are floristically homogenised or differentiated over time according to their flooding conditions and, hence, according to the area from which they receive surface and groundwater flow. Also, results corroborate the way the water regime determines the structure and heterogeneity of plant communities in such environments.
Is there an inherent conflict in managing fire for people and conservation?
Authors: Bentley P. D. , Penman T. D.
Published: June 2017

Wildfires are a natural disturbance in many ecosystems, creating challenges for land management agencies who need to simultaneously reduce risk to people and maintain ecological values. Here we use the PHOENIX RapidFire fire behaviour simulator to compare fuel treatment strategies that meet the twin objectives of reducing wildfire risk to human settlements and a fire sensitive endangered species, the koala (Phascolarctos cinereus) in south-eastern Australia. The local koala population is in decline and a conservation management plan is being prepared to exclude wildfire for a 10-year period to assist with population recovery. Twelve scenarios developed by the land management agencies were compared using four indicators: wildfire size; burn probability; impact from exposure to fire; and treatment cost. Compared with the current risk setting, three treatment scenarios were found to reduce wildfire size and burn probability concurrently to both people and koalas. These strategies worked by increasing the landscape area treated, which came with increased financial cost. However, the impact from exposure to fire for both property and koala habitat remains high. Additional complementary strategies beyond landscape fuel reductions are needed to reduce impact from exposure in the event of a wildfire.