bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction Models for Prognosis of Cervical Cancer: Systematic Review and Critical Appraisal
Authors: He Bingjie , Chen Weiye , Liu Lili , Hou Zheng , Zhu Haiyan , Cheng Haozhe , Zhang Yixi , Zhan Siyan , Wang Shengfeng
Published: May 2021

Objective: This work aims to systematically identify, describe, and appraise all prognostic models for cervical cancer and provide a reference for clinical practice and future research.

Methods: We systematically searched PubMed, EMBASE, and Cochrane library databases up to December 2020 and included studies developing, validating, or updating a prognostic model for cervical cancer. Two reviewers extracted information based on the CHecklist for critical Appraisal and data extraction for systematic Reviews of prediction Modeling Studies checklist and assessed the risk of bias using the Prediction model Risk Of Bias ASsessment Tool.

Results: Fifty-six eligible articles were identified, describing the development of 77 prognostic models and 27 external validation efforts. The 77 prognostic models focused on three types of cervical cancer patients at different stages, i.e., patients with early-stage cervical cancer (n = 29; 38%), patients with locally advanced cervical cancer (n = 27; 35%), and all-stage cervical cancer patients (n = 21; 27%). Among the 77 models, the most frequently used predictors were lymph node status (n = 57; 74%), the International Federation of Gynecology and Obstetrics stage (n = 42; 55%), histological types (n = 38; 49%), and tumor size (n = 37; 48%). The number of models that applied internal validation, presented a full equation, and assessed model calibration was 52 (68%), 16 (21%), and 45 (58%), respectively. Twenty-four models were externally validated, among which three were validated twice. None of the models were assessed with an overall low risk of bias. The Prediction Model of Failure in Locally Advanced Cervical Cancer model was externally validated twice, with acceptable performance, and seemed to be the most reliable.

Conclusions: Methodological details including internal validation, sample size, and handling of missing data need to be emphasized on, and external validation is needed to facilitate the application and generalization of models for cervical cancer.
Cough Recognition Based on Mel-Spectrogram and Convolutional Neural Network
Authors: Zhou Quan , Shan Jianhua , Ding Wenlong , Wang Chengyin , Yuan Shi , Sun Fuchun , Li Haiyuan , Fang Bin
Published: May 2021

In daily life, there are a variety of complex sound sources. It is important to effectively detect certain sounds in some situations. With the outbreak of COVID-19, it is necessary to distinguish the sound of coughing, to estimate suspected patients in the population. In this paper, we propose a method for cough recognition based on a Mel-spectrogram and a Convolutional Neural Network called the Cough Recognition Network (CRN), which can effectively distinguish cough sounds.
A COVID-19 Emergency Response for Remote Control of a Dialysis Machine with Mobile HRI
Authors: Wazir Hassam Khan , Lourido Christian , Chacko Sonia Mary , Kapila Vikram
Published: May 2021

Healthcare workers face a high risk of contagion during a pandemic due to their close proximity to patients. The situation is further exacerbated in the case of a shortage of personal protective equipment that can increase the risk of exposure for the healthcare workers and even non-pandemic related patients, such as those on dialysis. In this study, we propose an emergency, non-invasive remote monitoring and control response system to retrofit dialysis machines with robotic manipulators for safely supporting the treatment of patients with acute kidney disease. Specifically, as a proof-of-concept, we mock-up the touchscreen instrument control panel of a dialysis machine and live-stream it to a remote user’s tablet computer device. Then, the user performs touch-based interactions on the tablet device to send commands to the robot to manipulate the instrument controls on the touchscreen of the dialysis machine. To evaluate the performance of the proposed system, we conduct an accuracy test. Moreover, we perform qualitative user studies using two modes of interaction with the designed system to measure the user task load and system usability and to obtain user feedback. The two modes of interaction included a touch-based interaction using a tablet device and a click-based interaction using a computer. The results indicate no statistically significant difference in the relatively low task load experienced by the users for both modes of interaction. Moreover, the system usability survey results reveal no statistically significant difference in the user experience for both modes of interaction except that users experienced a more consistent performance with the click-based interaction vs. the touch-based interaction. Based on the user feedback, we suggest an improvement to the proposed system and illustrate an implementation that corrects the distorted perception of the instrumentation control panel live-stream for a better and consistent user experience.
School Based Tooth Brushing and Annual Silver Diammine Fluoride Application as a Highest Priority Package for Achieving Universal Oral Health Care for Cambodian Children
Authors: Turton Bathsheba , Patel Jilen , Sieng Chanthyda , Tak Ranuch , Durward Callum
Published: May 2021

Background:Achieving Universal Oral Health Care among Low-to Middle-Income settings is challenging and little literature exists around exploring what a “Highest Priority Package” of care might look like in the context of oral health. The Healthy Kids Cambodia (HKC) program differs from most conventional school dental services in that the initial package of care that is offered is daily toothbrushing with 1,500 ppm fluoride toothpaste (DTB) together with the topical application of Silver Diamine fluoride (SDF) for management of lesions in primary teeth.

Aim:To examine tooth level outcomes for 8- to 10-year old children from two schools that performed DTB with application of SDF at differing time-points.

Design:This was an observational cohort study that examined lesion progression among children in late mixed dentition at two schools. Data were collected using the dmft and pufa indices. Both schools received materials and training for DTB at baseline. School One received SDF at baseline while School Two received SDF after 9-months. Intraoral examinations were performed and the presentation of primary teeth with cavitated carious lesions were compared at baseline and 12 m. If a tooth was still caries-active or had become pulpally involved, this was considered to be an unacceptable outcome. Descriptive analysis was performed the chi-squared test was used to examine differences in the proportion of teeth with unacceptable outcomes by school membership.

Results:Of the 521 children recruited, 470 (90.2%) were followed. Where there was a delay in SDF application (School 2) there was a three times greater chance of an unacceptable outcome. Ten percentage of primary teeth in School One and 33% of primary teeth in the School Two had unacceptable outcomes.

Conclusion:The present study offers data on expected effect sizes that might inform future step-wedged clinical trials to validate an oral health Highest Priority Package of care for Cambodian children. The delivery of a package of care that includes both DTB and SDF can prevent adverse outcomes, such as dental infections, in primary teeth with carious lesions.
Dental Care of Patients With Dementia: A Survey on Practice Equipment, Training, and Dental Treatment
Authors: Schaper Sophie , Meyer-Rötz Sinja , Bartels Claudia , Wiltfang Jens , Rödig Tina , Schott Björn H. , Belz Michael
Published: May 2021

For patients with dementia, dental care can pose a considerable challenge due to cognitive impairment, behavioral, and psychological symptoms, and (often subsequently) limited autonomous oral care. In this study, we aimed to assess the proficiency of dentists in general practice in the outpatient dental care of these patients. A total of 119 dentists from private practices in Lower Saxony, Germany, participated in this study. Concerning treatment of patients with dementia, they provided details about (1) practice equipment/consultation, (2) training/expertise, and (3) special circumstances of dental treatment. Participating dentists predominantly reported to use medical aids (e.g., positioning cushions) to improve the treatment situation for patients with dementia. Over two thirds (68.6%) offered consultations in nursing homes, and at the patients' homes (47.0%). The training rate was remarkably low in the field of gerodontology for dentists and their practice staff (<10%), however, 54.5% expressed interest in such training. The majority of dentists reportedly adapted their treatment strategy to the needs of patients with dementia (e.g., communication, inclusion of caregivers, time management). Furthermore, most participants adapted dental treatment adequately (e.g., strict indication for tooth extraction, simple design of dental prostheses). In summary, even though training in the field of gerodontology must be considered insufficient, most dentists in this study showed an adequate adaptation of their treatment strategy as well as consideration of dental characteristics in patients with dementia, along with interest in trainings. We conclude that dementia-specific training should be expanded in the field of dentistry, preferably already at university level.
Enhanced Eye Velocity in Head Impulse Testing—A Possible Indicator of Endolymphatic Hydrops
Authors: Curthoys Ian S. , Manzari Leonardo , Rey-Martinez Jorge , Dlugaiczyk Julia , Burgess Ann M.
Published: May 2021

Introduction: On video head impulse testing (vHIT) of semicircular canal function, some patients reliably show enhanced eye velocity and so VOR gains >1.0. Modeling and imaging indicate this could be due to endolymphatic hydrops. Oral glycerol reduces membranous labyrinth volume and reduces cochlear symptoms of hydrops, so we tested whether oral glycerol reduced the enhanced vHIT eye velocity.

Study Design: Prospective clinical study and retrospective analysis of patient data.

Methods: Patients with enhanced eye velocity during horizontal vHIT were enrolled (n = 9, 17 ears) and given orally 86% glycerol, 1.5 mL/kg of body weight, dissolved 1:1 in physiological saline. Horizontal vHIT testing was performed before glycerol intake (time 0), then at intervals of 1, 2, and 3 h after the oral glycerol intake. Control patients with enhanced eye velocity (n = 4, 6 ears) received water and were tested at the same intervals. To provide an objective index of enhanced eye velocity we used a measure of VOR gain which captures the enhanced eye velocity which is so clear on inspecting the eye velocity records. We call this measure the initial VOR gain and it is defined as: (the ratio of peak eye velocity to the value of head velocity at the time of peak eye velocity). The responses of other patients who showed enhanced eye velocity during routine clinical testing were analyzed to try to identify how the enhancement occurred.

Results: We found that oral glycerol caused, on average, a significant reduction in the enhanced eye velocity response, whereas water caused no systematic change. The enhanced eye velocity during the head impulses is due in some patients to a compensatory saccade-like response during the increasing head velocity.

Conclusion: The significant reduction in enhanced eye velocity during head impulse testing following oral glycerol is consistent with the hypothesis that the enhanced eye velocity in vHIT may be caused by endolymphatic hydrops.
Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine
Authors: Mendoza Noelia , Casao Adriana , Domingo Juan , Quintín Francisco , Laviña Adolfo , Fantova Enrique , Cebrián-Pérez José Álvaro , Muiño-Blanco Teresa , Pérez-Pe Rosaura
Published: May 2021

The prediction of the fertilizing ability of a seminal dose continues to be a primary aim in the field of artificial insemination (AI). To achieve this goal, in this study we have included the evaluation of some non-conventional sperm quality markers. A total of 3,906 ewes from 52 different farms were inseminated with 357 refrigerated seminal doses obtained from 45 matureRasa Aragonesarams. The same samples were used for sperm quality analysis including membrane integrity, capacitation status, oxygen consumption and apoptotic-like markers such as phosphatidylserine translocation (PS), plasmalemma disorganization/mitochondrial membrane potential, caspase activation and DNA damage. Seminal doses from the breeding (B) season presented higher percentages of intact membrane (IM), non permeant (NP) membrane with high mitochondrial membrane potential (ΔΨm) and IM without PS translocation spermatozoa than those from the non-breeding (NB) season. Therefore, we can conclude that there were less spermatozoa showing apoptotic-like features in the seminal doses from the B than the NB season, although these differences did not affect field fertility. Only the percentage of intact membrane, non-capacitated (IM-NC) spermatozoa showed a significant correlation within vivofertility (P= 0.005) and fecundity (P= 0.007) values obtained after cervical AI when all data were evaluated. When the data were sorted by season and distance to the farms where AI was performed, the correlation between the percentage of IM-NC spermatozoa and reproductive parameters increased in the NB season and progressively with remoteness from the farms. Some other sperm parameters, like NP with high ΔΨm, IM sperm without active caspases and DNA-intact spermatozoa, also showed significant correlations with the reproductive parameters in the sorted data. Moreover, the increment in both the percentage of IM-NC and DNA-intact spermatozoa would increase the probability of obtaining a fertility higher than the mean (>52%), as revealed by a multiple logistic regression analysis. In conclusion, we have identified two seminal markers—the percentage of intact membrane, non-capacitated spermatozoa, and DNA intact spermatozoa—which could be used as a test to discard males in AI programs, which is highly important from an economic point of view and can contribute to achieving satisfactory fertility rates.
Genetic Characterization of CTX-M-2-Producing Klebsiella pneumoniae and Klebsiella oxytoca Associated With Bovine Mastitis in Japan
Authors: Tsuka Takeshi , Ozaki Hiroichi , Saito Daisuke , Murase Toshiyuki , Okamoto Yoshiharu , Azuma Kazuo , Osaki Tomohiro , Ito Norihiko , Murahata Yusuke , Imagawa Tomohiro
Published: May 2021

CTX-M-2-producingKlebsiella oxytoca(K. oxytoca) has not received much attention in animal husbandry compared withKlebsiella pneumoniae(K. pneumoniae), a major reservoir of extended-spectrum β-lactamase (ESBL) genes. Bacteriological examinations of 1,466 mastitic milk samples between October 2012 and December 2014 were conducted. Ninety-fiveK. pneumoniaeisolates (total prevalence: 6.5%) and 81K. oxytocaisolates (total prevalence: 5.5%) were obtained. SeventeenK. pneumoniaeisolates obtained from 15 animals reared on 11 farms and 9K. oxytocaisolates obtained from 9 animals reared on the same farm were phenotypically confirmed to be ESBL producers. All nine ESBL-producingK. oxytocaisolates were obtained from one farm between June and November 2013 and related to a significantly (p< 0.05) higher monthly prevalence of mild mastitis (in June, August, September, October, and November 2013). Pulsed-field gel electrophoresis (PFGE) patterns of ESBL-producingK. pneumoniaeisolates were distinguished from each other by more than 6-band differences except for two isolates from two animals, whereas all nineK. oxytocaisolates showed an identical PFGE pattern. Transferability of theblaCTX−M−2gene was found in 14K. pneumoniaeand 9K. oxytocaisolates by conjugation analysis. Of these isolates, theblaCTX−M−2gene was detected on plasmids belonging to the incompatibility (Inc) groups P and N derived from fiveK. pneumoniaeand nineK. oxytocaisolates, respectively, although the plasmids from the remaining nineK. pneumoniaewere untypeable. All the transconjugants exhibited elevated minimum inhibitory concentrations of ampicillin, cefotaxime, and ceftiofur compared with those in the wild-type, recipient strain. Restriction fragment length polymorphism analysis demonstrated that the IncN plasmids extracted from eight of nine transconjugants, which received resistance against β-lactams fromK. oxytoca, showed an identicalDraI digestion pattern. These results suggest that the CTX-M-2-producingK. oxytocastrain with the above-mentioned characteristics may have clonally spread within a farm, whereas theblaCTX−M−2gene inK. pneumoniaepossibly disseminated among the farms through different plasmids. Thus, monitoring of ESBL genes, including theblaCTX−M−2gene, among causative agents of bacterial mastitis in cows can help to develop relevant treatments and control practices.
The Effects of Resuscitative Fluid Therapy on the Endothelial Surface Layer
Authors: Smart Lisa , Hughes Dez
Published: May 2021

The goal of resuscitative fluid therapy is to rapidly expand circulating blood volume in order to restore tissue perfusion. Although this therapy often serves to improve macrohemodynamic parameters, it can be associated with adverse effects on the microcirculation and endothelium. The endothelial surface layer (ESL) provides a protective barrier over the endothelium and is important for regulating transvascular fluid movement, vasomotor tone, coagulation, and inflammation. Shedding or thinning of the ESL can promote interstitial edema and inflammation and may cause microcirculatory dysfunction. The pathophysiologic perturbations of critical illness and rapid, large-volume fluid therapy both cause shedding or thinning of the ESL. Research suggests that restricting the volume of crystalloid, or “clear” fluid, may preserve some ESL integrity and improve outcome based on animal experimental models and preliminary clinical trials in people. This narrative review critically evaluates the evidence for the detrimental effects of resuscitative fluid therapy on the ESL and provides suggestions for future research directions in this field.
The Urban and Peri-Urban Farms (UPFs) Relational Model: The Case of Greater Poland Voivodeship, Poland
Authors: Wiśniewska-Paluszak Joanna , Paluszak Grzegorz
Published: May 2021

Different studies demonstrate that urban and peri-urban agriculture (UPA) operate in so-called alternative food networks (AFNs). However, very little is known about UPA’s relational business models. Therefore, this paper investigates how the urban and peri-urban farms (UPFs) form business relationships in food networks. Their business and social relationships are much more comprehensive than traditional transactional relationships. Therefore, this study aims to delimitate the relational model of UPFs. The managers and owners of nine UPFs in Greater Poland Voivodeship, Poland, in 2019 and 2020 were investigated. To this end, we employed a triple triangulation approach—methodological, theoretical, and investigator. The study refers primarily to the theory of inter-organisational relations (IORs); the empirical analysis adopts a network approach and activities–resources–actors (ARA) model framework. The study identifies UPFs business relationships and the main features of their networks, which led to the development of a conceptual relational UPFs model. This shows the diversification of activities, the competitiveness of resources and formality of organisational structures as a basis of a farm business, the integration of activities, the sharing of resources, and the collaboration of actors as the basis of the relationship. Finally, this shows the joint nature of activities—locality of resources and mutual trust among actors—as the basis of UPFs networking.
You Say You Want a Data Revolution? Taking on Food Systems Accountability
Authors: Marshall Quinn , Bellows Alexandra L. , McLaren Rebecca , Jones Andrew D. , Fanzo Jessica
Published: May 2021

Dramatic improvements in data availability and quality are needed to meet the challenge of monitoring and analyzing food systems, so that appropriate policies and actions to improve human and planetary health can be identified and data-informed accountability mechanisms put in place to strengthen food systems governance. Studying food systems is complex due to diverse actors and interlinking processes that operate on multiple spatial and temporal scales, and their multiple outcomes, which may be subject to hidden feedback mechanisms and tradeoffs. However, descriptive research to characterize food system components and make comparisons across geography, income groups, and population groups is an important foundation. The first part of this article details a series of critical data gaps and limitations that are currently hindering food systems learning and accountability, also comparing these gaps across regions and income groups. The second part of the article introduces the Food Systems Dashboard, a new data visualization tool that aims to improve access to and usage of food systems-related data, thus strengthening the data value chain and better informing policies and actions intended to improve diets, nutrition, livelihoods, and environmental sustainability.
Long-Term Dynamics of Land Use in the Romanian Plain—The Central Bărăgan, Romania
Authors: Ovreiu Adriana Bianca , Bărsoianu Iulian Andrei , Nistor Constantin , Nedelea Alexandru , Comănescu Laura
Published: May 2021

Changes in land use and agricultural landscapes are primarily the result of socio-political and economic changes. This research is based on the analysis of old maps, pertaining to different historical periods, aiming to capture the dynamics of the landscape in the Central Bărăgan Plain. The cartographic materials used have the Map of Southern Romania from 1864, the Firing Master Plans and the Corine Land Cover dataset (1990, 2000, 2018) as reference. For the case studies, these sources are supplemented by the orthophotoplan from 2012 and the remotely-sensed image acquired by Corona satellites in 1974. The results highlight the fact that between 1864 and 2018, the Central Bărăgan Plain recorded important changes in land resources. The most significant transformations took place in the period between 1864 and 1959, when 58% of the plain area was purposed for another use in that large areas of land were introduced in the agricultural use. If in 1864 the agricultural lands represented less than half (196.896 ha) of the Central Bărăgan area, the natural areas being dominant (201.473 ha), in the first half of the 20th century, they increased exponentially (361.674 ha), the natural vegetation occupying much smaller areas (28.481 ha) mainly along the Ialomița and Călmățui rivers and near lakes. This trend is also expressed by the values of the index of naturalness which shows a drastic decrease, from 51.7% in 1864, to 10.6% in 1916–1959. There is also an increase of the area cover by settlements which should be explained by the occurrence of new villages and the increase in size of the existing villages. Another result of the approach concerns the changes that occurred in the agricultural landscape in the middle, respectively at the end of the 20th century, which is a fact quantified by computing the index of land fragmentation. The dynamics of land use and the changes in the features of the agricultural landscape, as far as the analyzed area is concerned, are due to the agrarian reforms pertaining to different historical periods, as well as to the forced relocations during the communist regime. Therefore, the study aims to highlight changes during historical, socio-economic and political time in land use and to reduce the degree of naturalness of the territory at the same time.
New Trends in Disease and Pest Management: Challenges and Opportunities
Authors: González-Domínguez Elisa , Monzó César , Vicent Antonio
Published: May 2021

Horticultural and field crops are affected by a number of pests and diseases caused by arthropods, bacteria, fungi, oomycetes, viruses, virus-like organisms, and nematodes [...]
Effects of Pasture Grass, Silage, and Hay Diet on Equine Fecal Microbiota
Authors: Zhu Yiping , Wang Xuefan , Deng Liang , Chen Shulei , Zhu Chunyan , Li Jing
Published: May 2021

Diet is an important factor affecting intestinal microbiota in horses. Fecal microbiota is commonly used as a substitute for studying hindgut microbiota when investigating the relationship between intestinal microbial changes and host health. So far, no study has compared the difference between the fecal microbiota found in horses that are fed pasture grass, silage, and hay. The present study aims to characterize the fecal microbiota in horses that were exclusively on one of the three forage diets, and to analyze the potential impact of these forages, especially silage, on horse intestinal health. There were 36 horses randomly assigned to each of the three groups; each group was fed only one type of forage for 8 weeks. High throughput sequencing was applied to analyze the bacterial taxa in fecal samples collected from the horses at the end of the feeding trial. The Lachnospiraceae family was statistically more abundant in horses fed with hay, while it was the least abundant in horses fed with silage. The Streptococcaceae spp., considered a core microbial component in equine intestinal microbiota, were present in significantly lower quantities in feces from horses that were fed pasture grass as compared to those from horses fed hay or silage. The novel data may help promote the balancing of horse intestinal microbiota and the maintenance of intestinal health in horses.
Quantifying the Impact of Mounted Load Carrying on Equids: A Review
Authors: Bukhari Syed S. U. H. , McElligott Alan G. , Parkes Rebecca S. V.
Published: May 2021

There are approximately 112 million working equids in developing countries, many of which are associated with brick kilns. Brick kilns and overloading are associated with welfare problems in working equids. Understanding equids’ abilities and influencing factors are important for both effective performance and welfare. Traditionally, measurement of the amount of ‘bone’ was used, and more recently, gait symmetry has been identified as a potential marker for loading capacity. Assessment of stride parameters and gait kinematics provides insights into adaptations to loading and may help determine cut-off loads. Physiological factors such as the ability to regain normal heart rates shortly after work is an important tool for equine fitness assessment and a more accurate measure of load-carrying capacity than absolute heart rate. Oxidative stress, plasma lactate, and serum creatine kinase activity are reliable biochemical indicators of loading ability. For monitoring stress, salivary cortisol is superior to serum cortisol level for assessment of hypothalamus-pituitary-adrenal axis and is related to eye temperatures, but this has yet to be interpreted in terms of load-carrying ability in equids. Further research is needed to standardize the evidence-based load-carrying capacity of working horses and donkeys.
Olive Leaf Extract Supplementation to Old Wistar Rats Attenuates Aging-Induced Sarcopenia and Increases Insulin Sensitivity in Adipose Tissue and Skeletal Muscle
Authors: González-Hedström Daniel , Priego Teresa , Amor Sara , de la Fuente-Fernández María , Martín Ana Isabel , López-Calderón Asunción , Inarejos-García Antonio Manuel , García-Villalón Ángel Luís , Granado Miriam
Published: May 2021

Aging is associated with increased visceral adiposity and a decrease in the amount of brown adipose tissue and muscle mass, known as sarcopenia, which results in the development of metabolic alterations such as insulin resistance. In this study, we aimed to analyze whether 3-week supplementation with a phenolic-rich olive leaf extract (OLE) to 24 months-old male Wistar rats orally (100 mg/kg) attenuated the aging-induced alterations in body composition and insulin resistance. OLE treatment increased brown adipose tissue and attenuated the aging-induced decrease in protein content and gastrocnemius weight. Treatment with OLE prevented the aging-induced increase in the expression of PPAR-γ in visceral and brown adipose tissues, while it significantly increased the expression of PPAR-α in the gastrocnemius of old rats and reduced various markers related to sarcopenia such as myostatin, HDAC-4, myogenin and MyoD. OLE supplementation increased insulin sensitivity in explants of gastrocnemius and epididymal visceral adipose tissue from aged rats through a greater activation of the PI3K/Akt pathway, probably through the attenuation of inflammation in both tissues. In conclusion, supplementation with OLE prevents the loss of muscle mass associated with aging and exerts anti-inflammatory and insulin-sensitizing effects on adipose tissue and skeletal muscle.
Applied Biomechanics: Sport Performance and Injury Prevention
Authors: Navarro Enrique , Navandar Archit , Veiga Santiago , San Juan Alejandro F.
Published: May 2021

This Special Issue had, as its main objective, the compilation of studies on sports performance and its relationship with musculoskeletal injuries [...]
Exposure to Phthalates and Alternative Plasticizers Is Associated with Methylation Changes of ESR1 and PGR in Uterine Leiomyoma: The ELENA Study
Authors: Cho Yoon Hee , Yoon Yeong Sook , Koo Min Sun , Kim Wanseo , Kho Younglim , Kim Sunmi , Kim Yang Jee , Choi Haewon , Choi Eun Jeong , Koh Jae Whoan , Chun Kyoung Chul , Kim Young Ah
Published: May 2021

Uterine leiomyomas are estrogen-dependent benign tumors with unknown etiologies. Phthalates are endocrine-disrupting chemicals and ubiquitous in the environment; thus, it has been suggested that they play a role in the development of uterine leiomyoma. We aimed to investigate whether the pathogenesis of uterine leiomyoma is related to methylation changes in promoter regions of estrogen receptor α (ESR1) and progesterone receptor (PGR) genes in response to phthalates and alternative plasticizers exposure. Urinary concentrations of 20 phthalate metabolites and seven metabolites of di-2-ethylhexyl terephthalate (DEHTP) and di (isononyl) cyclohexane-1,2-dicarboxylate (DINCH) were measured by UHPLC-MS/MS in thirty leiomyoma patients, who provided both paired leiomyoma and myometrium tissues. Methylation levels of ESR1 and PGR were analyzed by pyrosequencing assay. A total of 12 phthalate metabolites and 5 alternative metabolites (3 DEHTP and 2 DINCH) were detected >70% among study participants. The methylation of ESR1 and PGR were significantly lower in leiomyoma tissues compared to those in myometrium (18.10 ± 4.41 vs. 28.72 ± 4.95; 2.32 ± 0.81 vs. 3.27 ± 0.56, respectively). ESR1 methylation in leiomyoma was negatively associated with mono-2-carboxylmethyl-hexyl phthalate (2cx-MMHP) and mono-3-carbocyl-propyl phthalate (MCPP) after adjusting for confounding factors. However, 1-mono-2-ethyl-5-oxohexyl-benzene-1,4-dicarboxylate (5OXO-MEHTP), one of the alternatives, showed positive association with ESR1 methylation in leiomyoma. PGR methylation in leiomyoma was significantly associated with mono butyl phthalate (MnBP), but negatively associated with cyclohexane-1,2-dicarboxylate-mono-7-hydroxy-4-methyl-heptyl ester (cx-MINCH). Our results suggest that phthalates exposure may contribute to leiomyoma pathogenesis via ESR1 and PGR methylation changes.
Low Doses of Gamma Irradiation Stimulate Synthesis of Bioactive Compounds with Antioxidant Activity in Fomes fomentarius Living Mycelium
Authors: Pelcaru Cristina Florentina , Ene Mihaela , Petrache Alina-Maria , Neguț Daniel Constantin
Published: May 2021

Environmental changes generate free radicals and reactive oxygen species (ROS), resulting in abiotic stress in plants and fungi. Gamma ionizing radiation generates a significant amount of free radicals and ROS, thereby simulating natural environmental stressors. We used a 60Co source of radiation to experimentally induce oxidative stress in living mycelium mass of the medicinal fungus Fomes fomentarius, in order to obtain a late response of stress tolerance by means of bioactive compounds synthesis. We measured the response at 24, 48, and 72 h after the irradiation. The highest improvement was found 24 h after exposure for antioxidant activity and for total phenolic compounds of methanolic extract, with a 1.89- and 1.64-fold increase, respectively. The total flavonoids in methanolic extract increased 1.68 times after 48 h from treatment and presented a more stable raising in the assessed time-lapse. For the three analyzed parameters, 300 Gy was the optimum absorbed dose to trigger a beneficial response, with potentially applications in pharmaceutics and nutraceutics. Gamma irradiation can be used as a biotechnological tool to direct the secondary metabolites synthesis upregulation in medicinal mushroom living mycelium.
A New Multi-Person Pose Estimation Method Using the Partitioned CenterPose Network
Authors: Wu Jiahua , Lee Hyo-Jong
Published: May 2021

In bottom-up multi-person pose estimation, grouping joint candidates into the appropriately structured corresponding instance of a person is challenging. In this paper, a new bottom-up method, the Partitioned CenterPose (PCP) Network, is proposed to better cluster the detected joints. To achieve this goal, we propose a novel approach called Partition Pose Representation (PPR) which integrates the instance of a person and its body joints based on joint offset. PPR leverages information about the center of the human body and the offsets between that center point and the positions of the body’s joints to encode human poses accurately. To enhance the relationships between body joints, we divide the human body into five parts, and then, we generate a sub-PPR for each part. Based on this PPR, the PCP Network can detect people and their body joints simultaneously, then group all body joints according to joint offset. Moreover, an improved l1 loss is designed to more accurately measure joint offset. Using the COCO keypoints and CrowdPose datasets for testing, it was found that the performance of the proposed method is on par with that of existing state-of-the-art bottom-up methods in terms of accuracy and speed.
Imaging Top of Volcanic Mounds Using Seismic Time- and Depth-Domain Data Processing
Authors: Son Woohyun , Cheong Snons , Lee Changyoon , Kang Moohee
Published: May 2021

A seismic survey identified a basalt flow that could consist of cap rock of CO2 storage beneath saline aquifer sediment in the Southern Continental Shelf of Korea. To determine the precise depth of the basalt flow, specific depth-domain data processing of migration velocity analysis (MVA) was applied to the seismic survey data. The accurate depth measurement of a target structure provides crucial information when storing and stabilizing injected CO2 beneath basalt cap rock. Strong reflections of seismic amplitude at the volcanic mounds were adjusted from the time domain to the exact depth domain by the iterated velocity using MVA. The confidence of the updated velocity was verified by the horizontal alignment of seismic events sorted according to their common reflection point (CRP). The depth difference in volcanic mounds before and after MVA application ranged from 32.5 to 60 m along the vertical axis, showing the eruption shape on the strong-amplitude contour map in detail. The eruption shape of the top of volcanic mounds was verified with spatial continuity in 3D geological interpretation. The presented results provide suitable information that can be used to locate drilling sites and to prepare CO2 injection. The geological model obtained from both time- and depth-domain processing can significantly influence the calculation of the storage volume and can be useful for history matching studies.
Development and Characterization of 3D Printed Multifunctional Bioscaffolds Based on PLA/PCL/HAp/BaTiO3 Composites
Authors: Mystiridou Emmanouela , Patsidis Anastasios C. , Bouropoulos Nikolaos
Published: May 2021

Bone substitute materials are placed in bone defects and play an important role in bone regeneration and fracture healing. The main objective of the present research is fabrication through the technique of 3D printing and the characterization of innovative composite bone scaffolds composed of polylactic acid (PLA), poly (ε-caprolactone) (PCL) while hydroxyapatite (HAp), and/or barium titanate (BaTiO3—BT) used as fillers. Composite filaments were prepared using a single screw melt extruder, and finally, 3D composite scaffolds were fabricated using the fused deposition modeling (FDM) technique. Scanning electron microscopy (SEM) images showed a satisfactory distribution of the fillers into the filaments and the printed objects. Furthermore, differential scanning calorimetry (DSC) measurements revealed that PLA/PCL filaments exhibit lower glass transition and melting point temperatures than the pure PLA filaments. Finally, piezoelectric and dielectric measurements of the 3D objects showed that composite PLA/PCL scaffolds containing HAp and BT exhibited piezoelectric coefficient (d33) values close to the human bone and high dielectric permittivity values.
Sharing Soil and Building Geophysical Data for Seismic Characterization of Cities Using CLARA WebGIS: A Case Study of Matera (Southern Italy)
Authors: Tragni Nicola , Calamita Giuseppe , Lastilla Lorenzo , Belloni Valeria , Ravanelli Roberta , Lupo Michele , Salvia Vito , Gallipoli Maria Rosaria
Published: May 2021

In the context of seismic risk, studying the characteristics of urban soils and of the built environment means adopting a holistic vision of the city, taking a step forward compared to the current microzonation approach. Based on this principle, CLARA WebGIS aims to collect, organize, and disseminate the available information on soils and buildings in the urban area of Matera. The geodatabase is populated with (i) 488 downloadable geological, geotechnical, and geophysical surveys; (ii) geological, geomorphological, and seismic homogeneous microzone maps; and (iii) a new Digital Surface Model. The CLARA WebGIS is the first publicly available database that reports for the whole urban area the spatial distribution of the fundamental frequencies for soils and the overlying 4043 buildings, along with probability levels of soil-building resonance. The WebGIS is aimed at a broad range of end users (local government, engineers, geologists, etc.) as a support to the implementation of seismic risk mitigation strategies in terms of urban planning, seismic retrofitting, and management of post-earthquake crises. We recommend that the database be managed by local administrators, who would also have the task of deciding on future developments and continuous updating as new data becomes available.
A Spatio-Temporal Visualization Approach of PM10 Concentration Data in Metropolitan Lima
Authors: Encalada-Malca Alexandra Abigail , Cochachi-Bustamante Javier David , Rodrigues Paulo Canas , Salas Rodrigo , López-Gonzales Javier Linkolk
Published: May 2021

Lima is considered one of the cities with the highest air pollution in Latin America. Institutions such as DIGESA, PROTRANSPORTE and SENAMHI are in charge of permanently monitoring air quality; therefore, the air quality visualization system must manage large amounts of data of different concentrations. In this study, a spatio-temporal visualization approach was developed for the exploration of data of the PM10 concentration in Metropolitan Lima, where the spatial behavior, at different time scales, of hourly concentrations of PM10 are analyzed using basic and specialized charts. The results show that the stations located to the east side of the metropolitan area had the highest concentrations, in contrast to the stations located in the center and north that reported better air quality. According to the temporal variation, the station with the highest average of biannual and annual PM10 was the HCH station. The highest PM10 concentrations were registered in 2018, during the summer, highlighting the month of March with daily averages that reached 435 μμg/m3. During the study period, the CRB was the station that recorded the lowest concentrations and the only one that met the Environmental Quality Standard for air quality. The proposed approach exposes a sequence of steps for the elaboration of charts with increasingly specific time periods according to their relevance, and a statistical analysis, such as the dynamic temporal correlation, that allows to obtain a detailed visualization of the spatio-temporal variations of PM10 concentrations. Furthermore, it was concluded that the meteorological variables do not indicate a causal relationship with respect to PM10 levels, but rather that the concentrations of particulate material are related to the urban characteristics of each district.
Nocturnal Boundary Layer Evolution and Its Impacts on the Vertical Distributions of Pollutant Particulate Matter
Authors: Shi Yu , Liu Lei , Hu Fei , Fan Guangqiang , Huo Juntao
Published: May 2021

To investigate the evolution of the nocturnal boundary layer (NBL) and its impacts on the vertical distributions of pollutant particulates, a combination of in situ observations from a large tethered balloon, remote sensing instruments (aerosol lidar and Doppler wind lidar) and an atmospheric environment-monitoring vehicle were utilized. The observation site was approximately 100 km southwest of Beijing, the capital of China. Results show that a considerable proportion of pollutant particulates were still suspended in the residual layer (RL) (e.g., the nitrate concentration reached 30 μg m−3) after sunset. The NBL height calculated by the aerosol lidar was closer to the top of the RL before midnight because of the pollutants stored aloft in the RL and the shallow surface inversion layer; after midnight, the NBL height was more consistent with the top of the surface inversion layer. As the convective mixing layer gradually became established after sunrise the following day, the pollutants stored in the nocturnal RL of the preceding night were entrained downward into the mixing layer. The early morning PM2.5 concentration near 700 m in the RL on 20 December decreased by 83% compared with the concentration at 13:34 on 20 December at the same height. The nitrate concentration also decreased significantly in the RL, and the mixing down of nitrate from the RL could contribute about 37% to the nitrate in the mixing layer. Turbulence activities still existed in the RL with the bulk Richardson number (Rb) below the threshold value. The corresponding increase in PM2.5 was likely to be correlated with the weak turbulence in the RL in the early morning.
Regenerable ZnO/GaAs Bulk Acoustic Wave Biosensor for Detection of Escherichia coli in “Complex” Biological Medium
Authors: Chawich Juliana , Hassen Walid , Elie-Caille Céline , Leblois Thérèse , Dubowski Jan
Published: May 2021

A regenerable bulk acoustic wave (BAW) biosensor is developed for the rapid, label-free and selective detection of Escherichia coli in liquid media. The geometry of the biosensor consists of a GaAs membrane coated with a thin film of piezoelectric ZnO on its top surface. A pair of electrodes deposited on the ZnO film allows the generation of BAWs by lateral field excitation. The back surface of the membrane is functionalized with alkanethiol self-assembled monolayers and antibodies against E. coli. The antibody immobilization was investigated as a function of the concentration of antibody suspensions, their pH and incubation time, designed to optimize the immunocapture of bacteria. The performance of the biosensor was evaluated by detection tests in different environments for bacterial suspensions ranging between 103 and 108 CFU/mL. A linear dependence between the frequency response and the logarithm of E. coli concentration was observed for suspensions ranging between 103 and 107 CFU/mL, with the limit of detection of the biosensor estimated at 103 CFU/mL. The 5-fold regeneration and excellent selectivity towards E. coli detected at 104 CFU/mL in a suspension tinted with Bacillus subtilis at 106 CFU/mL illustrate the biosensor potential for the attractive operation in complex biological media.
Development of a Prototype Lateral Flow Immunoassay of Cortisol in Saliva for Daily Monitoring of Stress
Authors: Panfilova Elizaveta
Published: May 2021

Emotional stress negatively affects the quality of a person’s daily life. From a physiological point of view, stress is expressed in the excitation of the hypothalamic–pituitary–adrenal cortex axis, which leads to the release of the hormone cortisol into the blood. We developed a lateral flow immunoassay to detect cortisol in human salivary fluid and tested it on 10 healthy volunteers daily for about one month (n = 293 saliva samples). Cortisol was detected in concentrations ranging from 1 to 70 ng/mL. Salivary cortisol levels were confirmed by ELISA. The straightness range of LFIA calibration was from 1 to 100 ng/mL. The diagnostic sensitivity of the method was 73%. It was found that in 3 out of 10 subjects, fluctuations in the level of cortisol in saliva partially corresponded to the subjectively assessed level of stress.
Toward BIM and LPS Data Integration for Lean Site Project Management: A State-of-the-Art Review and Recommendations
Authors: Sbiti Maroua , Beddiar Karim , Beladjine Djaoued , Perrault Romuald , Mazari Bélahcène
Published: May 2021

Over recent years, the independent adoption of lean construction and building information modeling (BIM) has shown improvements in construction industry efficiency. Because these approaches have overlapping concepts, it is thought that their synergistic adoption can bring many more benefits. Today, implementing the lean–BIM theoretical framework is still challenging for many companies. This paper conducts a comprehensive review with the intent to identify prevailing interconnected lean and BIM areas. To this end, 77 papers published in AEC journals and conferences over the last decade were reviewed. The proposed weighting matrix showed the most promising interactions, namely those related to 4D BIM-based visualization of construction schedules produced and updated by last planners. The authors also show evidence of the lack of a sufficiently integrated BIM–Last Planner System® framework and technologies. Thus, we propose a new theoretical framework considering all BIM and LPS interactions. In our model, we suggest automating the generation of phase schedule using joint BIM data and a work breakdown structure database. Thereafter, the lookahead planning and weekly work plan is supported by a field application that must be able to exchange data with the enterprise resource planning system, document management systems, and report progress to the BIM model.
Reply to Krupenko et al. Comment on “Lee et al. The Combination of Loss of ALDH1L1 Function and Phenformin Treatment Decreases Tumor Growth in KRAS-Driven Lung Cancer Cancers 2020, 12, 1382”
Authors: Lee Seon-Hyeong , Jeon Yoon , Kang Joon-Hee , Jang Hyonchol , Hong Kyeong-Man , Hong Dongwan , Lee Ho , Kim Soo-Youl
Published: May 2021

In the Cancers paper, we observed the increase ALDH1L1 protein expression following oncogenesis, as well as a therapeutic effect, by deleting the Aldh1l1 gene in KrasLA2 mice, a model of spontaneous non-small cell lung cancer (NSCLC) [...]