bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased levels of antibodies against heat shock proteins in stroke patients.
Authors: Banecka-Majkutewicz Zyta , Grabowski Michał , Kadziński Leszek , Papkov Aliaksei , Węgrzyn Alicja , Banecki Bogdan
Published: February 2019

Ischemic stroke is the second leading cause of death worldwide. One of the main risk factors of the ischemic stroke is atherosclerosis which is a chronic inflammatory and immune-mediated disease. Bacterial infections generate specific human antibodies against various antigens, including Hsps. It has been demonstrated that Hsps are selectively overexpressed in the atherosclerotic lesions. The amino acid sequence homology between human and bacterial Hsps may lead to an autoimmune response by immunological cross-reaction. Such immune response against Hsps overexpressed in the blood vessels under stressful conditions may contribute to inflammatory processes and subsequent development of atherosclerosis. In this study we determined the antibody levels against bacterial and human Hsp by ELISA in blood plasma obtained from stroke patients. Using ANOVA we analyzed levels of Hsp-antibodies in control and patient groups and correlate them with several stroke risk factors. The group of stroke patients had elevated levels of anti-Hsp antibodies compared to the control group. We also discovered an antibody level increase in patients that previously underwent another stroke. Our data provide evidence that autoimmunity could underlie formation of atherosclerosis plaque leading to stroke.
Type 1 fimbriae in commensal Escherichia coli derived from healthy humans.
Authors: Pusz Paweł , Bok Ewa , Mazurek Justyna , Stosik Michał , Baldy-Chudzik Katarzyna
Published: February 2019

Type 1 fimbriae are one of the most important factors of Escherichia coli adaptation to different niches in the host. Our study indicated that the genetic marker--fimH gene occurred commonly in commensal E. coli derived from healthy humans but expression of the type 1 fimbriae was not observed. Identification of fim structural subunit genes (fimA-fimH) and recombinase fimE and fimB genes showed that many of the strains were carrying an incomplete set of genes and the genes expression study revealed that in strains with complete set of fim genes, the fimC gene, encoding the chaperone protein, was not expressed.
Screening of lipase carriers for reactions in water, biphasic and pure organic solvent systems.
Authors: Hrydziuszko Zofia , Dmytryk Agnieszka , Majewska Paulina , Szymańska Katarzyna , Liesiene Jolanta , Jarzębski Andrzej , Bryjak Jolanta
Published: February 2019

In bioprocesses lipases are typically used in immobilized form, irrespective of type of reaction systems, to ensure an even distribution of catalysts in water restricted media and/or to facilitate separation and reuse. In these studies we report on the selection of appropriate enzyme-carrier preparation for hydrolysis reaction in aqueous and biphasic systems and transesterification in organic solvent. For this Candida rugosa lipase was bound by adsorption or covalent attachment onto various carriers to give 24 preparations. Selection of proper preparation was based on reactivity, thermal stability (4 h at 60°C), possibility of drying and operational stability in 17-23 successive batch processes of 4-nitrophenyl palmitate hydrolysis in water. Activity of preparations varied from 20 to 5100 U∙mL(-1) but the most stable preparations were those of moderate activity: bound by adsorption or covalent attachment to NH₂-Kieselgel or acrylic carrier (retained activity over 90%). Selected preparations were used for hydrolysis of ethyl (1-butyryloxyethyl)-phenylphosphinate in biphasic system, and, after drying, in ethyl (1-hydroxyethyl)-phenyl-phosphinate transesterification. In this study operational stability was the principal criterion of selection. In water system, lipase covalently bound to NH₂-Kieselgel was the best - preserved 50% of initial activity in consecutive batch processes. In biphasic system and lipase covalently bound to acrylic and NH₂-Kieselgel the values were 90 or 77%, respectively, whereas in organic solvent, when lipase was immobilized on NH₂-Kieselgel by adsorption, it was 50%. Thus, NH₂-Kieselgel appears to be an universal matrix for investigated lipase immobilization and can be used in all reaction systems.
The inhibition of methicillin-resistant Staphylococcus aureus by essential oils isolated from leaves and fruits of Schinus areira depending on their chemical compositions.
Authors: Celaya Liliana S , Alabrudzińska Marta H , Molina Ana C , Viturro Carmen I , Moreno Silvia
Published: February 2019

Schinus areira L. is a native plant from South America used for centuries in traditional medicine. Here, we investigate the antimicrobial activity of four essential oils extracted from leaves and fruits of S. areira exhibiting different chemical profiles. The antibacterial activity against the human pathogenic bacteria Staphylococcus aureus susceptible as well as methicillin resistant strain was assessed by the broth microdilution assay. The results showed that the limonene-rich oil extracted from the leaves and fruits have potent antibacterial effect on S. aureus ATCC 25923, while the α-phellandrene-rich fruit oil having a lower content of limonene showed the lowest antibacterial efficacy. In this work, for the first time, we demonstrated the bactericidal activity of essential oils isolated from fruits and leaves of S. areira against susceptible and methicillin resistant S. aureus strains. All results point out the potential use of the S. areira oils as antimicrobial agents to be used, at least against Staphylococcal infections.
Role of biochemical factors in the pathogenesis of keratoconus.
Authors: Wojcik Katarzyna A , Blasiak Janusz , Szaflik Jerzy , Szaflik Jacek P
Published: February 2019

Keratoconus (KC) is a corneal disease associated with structural abnormalities in the corneal epithelium, Bowman's layer and stroma and altered concentration of tear components. KC corneas show a different pattern of collagen lamellae than their normal counterparts. Also, a reduction of several collagen types in KC epithelium and stroma was observed. Altered expression and/or activity of lysyl oxidase, a critical enzyme of the biogenesis of connective tissue detected in KC corneas, may weaken covalent bonds between collagen and elastin fibrils, what may lead to biomechanical deterioration of the cornea. Increased activity of matrix metalloproteinases observed in KC may induce the degradation of the extracellular matrix causing damage to the cornea. Oxidative and nitrative stress play an important role in KC pathogenesis and KC corneas are characterized by the disturbed lipid peroxidation and nitric oxide pathways. Malfunctioning of these pathways may lead to accumulation of their toxic by-products inducing several detrimental effects, along with apoptosis of the corneal cells, which may result from the loss of β-actin or increased levels of cytokines, including interleukin-1 and -6. Change in the expression of genes associated with wound healing, including the nerve growth factor and the visual system homeobox 1, may contribute to increased susceptibility of KC corneas to injury. Consequently, biochemical changes may play an important role in KC pathophysiology and, therefore, can be considered in prevention, diagnosis, prognosis and in the therapy of this disease as well.
Polymorphic variants of MIF gene and prognosis in steroid therapy in children with idiopathic nephrotic syndrome.
Authors: Świerczewska Monika , Ostalska-Nowicka Danuta , Kempisty Bartosz , Szczepankiewicz Aleksandra , Nowicki Michał
Published: February 2019

Nephrotic syndrome (NS) is the most common reason of proteinuria in children and can be caused by the pathology of renal glomeruli. Steroid therapy is typically used in this disorder. It has been shown that MIF is a cytokine which counteracts the immunosuppressive properties of glucocorticoids. The aim of this study was looking for a correlation between MIF polymorphisms and genetic susceptibility to steroid resistance in children with INS (Idiopathic NS).
The study was performed in 71 patients with INS including SRNS (steroid resistance nephrotic syndrome) (41) and SSNS (steroid sensitive nephrotic syndrome) (30) and in 30 control subjects. We employed Sanger sequencing and capillary electrophoresis. Linkage disequilibrium was made using Haploview and PHASE.
We didn't observe a statistical significance between SNPs detected in patients with INS and controls. Our studies revealed statistical significance for two polymorphisms: rs2070767C > T and rs2000466T > G between patients with SRNS and SSNS. The results for rs34383331T > A are close to being statistically significant. Statistical significance was revealed for CATT5/CATT6 genotype in SRNS group vs SSNS group (OR=4.604, 95%CI=1.356-15.632, p=0.0168). We found that the frequency of 5/X-CATT genotype compared with X/X-CATT genotype was significantly higher in SRNS patients vs SSNS (OR=3.167, 95%CI=1.046-9.585, p=0.0426). In linkage disequilibrium analysis we didn't show involvement in susceptibility to INS and steroid sensitive phenotype.
Our results suggest that the role of MIF polymorphisms in the susceptibility to positive response to steroid therapy is still unresolved. It indicates that MIF may be involved in indirect and complex molecular mechanisms of steroid activity in hormone-dependent metabolic pathways in children with INS. Because of ambiguous findings, pleiotropic features of this cytokine require that more research should be undertaken.
Type IV resistant starch increases cecum short chain fatty acids level in rats.
Authors: Le Thanh-Blicharz Joanna , Anioła Jacek , Kowalczewski Przemysław , Przygoński Krzysztof , Zaborowska Zofia , Lewandowicz Grażyna
Published: February 2019

Resistant starches are type of dietary fibers. However, their physiological effects depend on the way they resist digestion in the gastrointestinal tract. The objective of this study was to examine the hypothesis that new type of RS4 preparations, of in vitro digestibility of about 50%, obtained by cross-linking and acetylation, acts as a prebiotic by increasing short chain fatty acids content in cecum digesta. The rats were fed with diet containing pregelatinized, cross-linked and acetylated starches as a main carbohydrate source. Pregelatinized, but not chemically modified, potato starch was used in the composition of the control diet. After two weeks of experiment the increase of short chain fatty acids contents in ceceum digesta was observed. The intake of starch A, cross-linked only with adipic acid, resulted in increase of about 40% of short chain fatty acids content, whereas starch PA cross-linked with sodium trimetaphosphate and adipic acid of about 50%. The utmost twofold increase was observed in the case of the production of propionic acid. In contrast, the content of butyric acid increased (12%) only as an effect of consumption of starch PA and even decreased (about 30%) in case of starch A. Both RS4 starches caused an increase of the production of acetic acid by more than 40%. No changes in serum biochemistry, liver cholesterol and organ weights of rats were stated.
Kinetic modeling of simultaneous saccharification and fermentation of corn starch for ethanol production.
Authors: Białas Wojciech , Czerniak Adrian , Szymanowska-Powałowska Daria
Published: February 2019

Fuel ethanol production, using a simultaneous saccharification and fermentation process (SSF) of native starch from corn flour, has been performed using Saccharomyces cerevisiae and a granular starch hydrolyzing enzyme. The quantitative effects of mash concentration, enzyme dose and pH were investigated with the use of a Box-Wilson central composite design protocol. Proceeding from results obtained in optimal fermentation conditions, a kinetics model relating the utilization rates of starch and glucose as well as the production rates of ethanol and biomass was tested. Moreover, scanning electron microscopy (SEM) was applied to investigate corn starch granule surface after the SFF process. A maximum ethanol concentration of 110.36 g/l was obtained for native corn starch using a mash concentration of 25%, which resulted in ethanol yield of 85.71%. The optimal conditions for the above yield were found with an enzyme dose of 2.05 ml/kg and pH of 5.0. These results indicate that by using a central composite design, it is possible to determine optimal values of the fermentation parameters for maximum ethanol production. The investigated kinetics model can be used to describe SSF process conducted with granular starch hydrolyzing enzymes. The SEM micrographs reveal randomly distributed holes on the surface of granules.
Changes in the mitochondrial network during ectromelia virus infection of permissive L929 cells.
Authors: Gregorczyk Karolina P , Szulc-Dąbrowska Lidia , Wyżewski Zbigniew , Struzik Justyna , Niemiałtowski Marek
Published: February 2019

Mitochondria are extremely important organelles in the life of a cell. Recent studies indicate that mitochondria also play a fundamental role in the cellular innate immune mechanisms against viral infections. Moreover, mitochondria are able to alter their shape continuously through fusion and fission. These tightly regulated processes are activated or inhibited under physiological or pathological (e.g. viral infection) conditions to help restore homeostasis. However, many types of viruses, such as orthopoxviruses, have developed various strategies to evade the mitochondrial-mediated antiviral innate immune responses. Moreover, orthopoxviruses exploit the mitochondria for their survival. Such viral activity has been reported during vaccinia virus (VACV) infection. Our study shows that the Moscow strain of ectromelia virus (ECTV-MOS), an orthopoxvirus, alters the mitochondrial network in permissive L929 cells. Upon infection, the branching structure of the mitochondrial network collapses and becomes disorganized followed by destruction of mitochondrial tubules during the late stage of infection. Small, discrete mitochondria co-localize with progeny virions, close to the cell membrane. Furthermore, clustering of mitochondria is observed around viral factories, particularly between the nucleus and viroplasm. Our findings suggest that ECTV-MOS modulates mitochondrial cellular distribution during later stages of the replication cycle, probably enabling viral replication and/or assembly as well as transport of progeny virions inside the cell. However, this requires further investigation.
Estação de Carregamento Rápido com Elemento Armazenador de Energia e Filtro Ativo de Harmônicos para Veículos Elétricos
Authors: Balen Gleisson , Reis Andrei Roberto , Pinheiro Humberto , Schuch Luciano
Published: February 2019

O aprimoramento da infraestrutura de carregamento dos veículos elétricos é fundamental para a sua ampla adoção. Dessa forma, este artigo propõe uma estação modular de carregamento rápido com filtro ativo de potência e elemento armazenador de energia para veículos elétricos. A estação é conectada em média tensão e composta por 10 pontos de carregamento de 60 kW cada um, em acordo com as normas vigentes para carregamento rápido. Normas as quais são inicialmente revisadas e descritas. O filtro ativo de potência é apresentado, analisado e projetado para reduzir harmônicas de corrente no ponto de conexão com a rede de distribuição primária. Além disso, é realizada a análise de estabilidade na conexão dos conversores. Os resultados experimentais no Typhoon HIL comprovam o funcionamento da estação em acordo com as exigências normativas internacionais.
Progress in plant genome sequencing: research directions
Authors: Bragina M. K. , Afonnikov D. A. , Salina E. A.
Published: February 2019

Since the first plant genome of Arabidopsis thaliana has been sequenced and published, genome sequencing technologies have undergone significant changes. New algorithms, sequencing technologies and bioinformatic approaches were adopted to obtain genome, transcriptome and exome sequences for model and crop species, which have permitted deep inferences into plant biology. As a result of an improved genome assembly and analysis methods, genome sequencing costs plummeted and the number of high-quality plant genome sequences is constantly growing. Consequently, more than 300 plant genome sequences have been published over the past twenty years. Although many of the published genomes are considered incomplete, they proved to be a valuable tool for identifying genes involved in the formation of economically valuable plant traits, for marker-assisted and genomic selection and for comparative analysis of plant genomes in order to determine the basic patterns of origin of various plant species. Since a high coverage and resolution of a genome sequence is not enough to detect all changes in complex samples, targeted sequencing, which consists in the isolation and sequencing of a specific region of the genome, has begun to develop. Targeted sequencing has a higher detection power (the ability to identify new differences/variants) and resolution (up to one basis). In addition, exome sequencing (the method of sequencing only protein-coding genes regions) is actively developed, which allows for the sequencing of non-expressed alleles and genes that cannot be found with RNA-seq. In this review, an analysis of sequencing technologies development and the construction of “reference” genomes of plants is performed. A comparison of the methods of targeted sequencing based on the use of the reference DNA sequence is accomplished.
Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis
Authors: Illarionova N. B. , Petrovski D. V. , Razumov I. A. , Zavyalov E. L.
Published: February 2019

Gliomas are the most common type of malignant brain tumors. Standard treatment of gliomas consists of surgical excision of the tumor with subsequent chemotherapy and radiotherapy. Tumor cells are characterized by rapid division with an increased uptake of glucose and its catabolism during glycolysis. To maintain rapid division, the level of glycolysis of the tumor cell is significantly increased, compared with normal cells. It is known that some nanoparticles (NP) have the property of accumulating in tumors. In particular, NPs of manganese oxide can penetrate into the brain and, with considerable accumulation, cause toxic effects. These facts served as a prerequisite for studying the effects of manganese oxide NPs on the viability of glioma cells. The purpose of this work was to study the effects of manganese oxide NPs, as well as their combination with gamma irradiation on the glycolysis of glioma cells. The cells were irradiated using the research radiobiological gamma-installation IGUR-1 based on 137Cs. The level of cell glycolysis was determined using the standard glycolytic stress test on a Seahorse XFp platform. Cell viability was determined using the ViaCount reagent staining of living and dead cells. Their count was performed using flow cytometry. We showed that the glycolysis of U-87 MG glioma cells was significantly reduced when incubated for 48 hours with manganese oxide NPs. Irradiation in combination with NPs or alone did not have significant effects on glycolysis of gliomas. Glioma incubation with manganese oxide NPs for 72 hours led to a significant reduction in cell viability. This study may be useful for the development of new therapies and diagnosis of gliomas.
Microspore embryogenesis in vitro: the role of stresses
Authors: Djatchouk T. I. , Khomyakova O. V. , Akinina V. N. , Kibkalo I. A. , Pominov A. V.
Published: February 2019

Gametic embryogenesis is one form of totipotency of plant cells, in which either male or female gametes are induced to form embryoids (sporophytes). Regeneration of haploid plants from embryoids and subsequent chromosome duplication result in doubled haploids and DH-lines. The production of haploids and doubled haploids (DHs) through gametic embryogenesis allows a single-stage development of complete homozygous lines from heterozygous plants. The development of effective haploid protocols to produce homozygous plants has a significant impact on plant breeding, shorting the time and costs required to establish new cultivars. There are several available methods to obtain haploids and DHs-lines, of which anther or isolated microspore culture in vitro are the most effective. Microspore embryogenesis is more commonly applied. This is in part because more male gametophytes are contained in a single anther compared to the single female gametophyte per embryo sac. Microspore embryogenesis is regarded as one of the most striking examples of plant cell totipotency. The switch of cultured microspores from gametophytic to sporophytic mode of development has been induced by stress treatments of various kinds applied to donor plants, inflorescences, buds, anthers or isolated microspores both in vivo and in  vitro. Physical or chemical pretreatments (cold and heat shock, sugar starvation, colchicine, n-butanol, gametocydes) act as a trigger for inducing the sporophytic pathway, preventing the gametophytic pathway development of microspore. The recent investigations have revealed that cold pretreatment during microspore reprogramming acts rather as an anti-stress factor alleviating the real stress caused by nutrient starvation of anthers or microspores isolated from donor plants. Under stress pretreatment a vacuolated and polarized microspore transformed into a depolarized and dedifferentiated cell, which is an obligatory condition for reprogramming their development. We summarize data concerning the role of various stresses in the induction of microspore embryogenesis and possible mechanisms of their action at cellular and molecular levels. Identification of new stresses allows creating efficient protocols of doubled haploid production for end-user application in the breeding of many important crops.
Epidermolysis bullosa House Austria as a role model for the care of a rare disease
Authors: Prodinger C. M. , Laimer M. , Bauer J. , Hintner H.
Published: February 2019

The evolution of the Epidermolysis bullosa (EB) House Austria in Salzburg has demonstrated from its beginning in 2005 in an exceptional way the establishment of an optimized health care for a hitherto neglected group of patients, suffering from a rare but devastating skin disease: Epidermolysis bullosa. Patients with this hereditary mechanobullous skin disease, characterized by a heterogenous clinical course, multisystemic manifestations and increased morbidity and mortality, find in the EB House Austria a multidisciplinary, medical and psychosocial, family-centered support, optimally customized to this condition and individualized to each patient. Its unique structure of four divisions (Outpatient Unit, Research Laboratory, Academy, Clinical Research and Study Center) has set the basis for the delivery of best medical practice and state-of-the-art care as well as the establishment/ performance of high quality and patient centered research and translational medicine. Initially the (ongoing) close collaboration with the powerful patient group and medical research charity “DEBRA Austria” that is dedicated to a multidimensional support of EB patients and their relatives living in Austria and neighboring countries, has enabled the construction of the EB House Austria. The acknowledgement of this institution as a successful model has been officially obtained in 2017 by its designation as a national Center of Expertise for Genodermatoses with special focus on EB and its inclusion into the European Reference Network (ERN) for Rare Skin Disorders in September 2018. Therefore, the history of the EB house is worth reviewing since it can be regarded as a role model for the care of other rare and multisystemic diseases.
Challenges of the differential diagnosis between the subtypes of the junctional epidermolysis bullosa: presentation of two clinical cases
Authors: Kotalevskaya Yu. Yu. , Marycheva N. M.
Published: February 2019

Background: Epidermolysis bullosa (EB) is a rare hereditary skin disease. It is subdivided into EB simplex (EBS), junctional EB (JEB), dystrophic EB (DEB) and Kindler syndrome. JEB is diagnosed in 2 per 1,000,000 of the population. There are few descriptions of clinical JEB cases in the literature. Clinical diagnosis of JEB and its subtypes is a challenge, especially in the early age. The paper presents 2 clinical cases of JEB in patients of the West Slavonic origin. Clinical case No. 1 was a girl of Ukrainian ethnicity, with confirmed definitive diagnosis of severe generalized JEB. Molecular genetic tests identified mutations of the LAMA3 gene that had not been described previously. The patient died at the age of 24 months from acute respiratory failure. When the patient was alive, her EB type and subtype was not possible to identify, because she had a combination of clinical manifestations typical for various JEB subtypes. Despite such symptoms as hoarse voice, stenoses, granulation tissue of typical location, laryngeal granulations, the girl was steadily gaining weight, with some periods of relative stabilization of the skin disease; she also had longer life longevity than was common for patients with severe generalized JEB. All this made a precise diagnosis difficult. Clinical case No. 2: an ethnic Russian boy with non-classified JEB. Molecular genetic testing helped to identify a homozygote mutation in the LAMA3 gene that had not been previously described; reliable determination of the subtype was not possible. The patient had mixed clinical manifestation similar both to generalized severe JEB and to laryngo-onycho-cutaneous (LOC) syndrome. During his lifetime, the patient was clinically diagnosed with Hallopeau acrodermatitis and LOC syndrome. The differential diagnostic problems were associated with the presence of signs not typical for each of the subtypes. Significant life longevity of the proband is not characteristic for severe generalized JEB (at the time of the publication the patient is 13 years old), whereas for LOC syndrome the absence of eye involvement is not typical, as well as severe laryngeal involvement at adolescence.

Conclusion: Detailed descriptions of phenotype of JEB subtypes including rare and minimal clinical signs can be useful to study the clinical manifestations and natural course of the disease, including its differential diagnosis.
Addressing Challenges of Patients with Chronic Pulmonary Disease Using Makerspace Environments
Authors: Fasolino Tracy
Published: February 2019

Patients with chronic progressive pulmonary disease suffer from physiological and psychological consequences. These patients experience functional disability depression and anxiety and decreased quality of life. Their ability to perform activities of daily living can be severely impaired. Direct patient care nurses are well suited to assist these patients and their caregivers, identifying challenges through interactions and active listening and assisting in the identification of solutions. Even simple solutions to everyday issues can reduce the disease burden for this population. This article describes how direct patient care nurses can address the challenges of these patients through an innovative environment known as a makerspace.
From the Archive—“I Am the Professor of Myself”: How Our Patients and Their Loved Ones Become Our Teachers and Models
Authors: Savett Laurence A.
Published: February 2019

As health care professionals, we commit ourselves to lifelong learning. In that quest, wise professionals know that our patients and their loved ones become our most important teachers.
Tekicihila Unpo (Love One Another): Confronting Human Trafficking With the Guidance of Traditional Lakota Wisdom in Nursing Practice
Authors: Fear Whitney
Published: February 2019

Human trafficking emerged as a serious social issue in North Dakota during the Bakken oil field boom in the western part of the state. The oil industry has seen a dramatic decrease in production in recent years. However, the presence of human trafficking continues to dominate the scene in the state. As the RN Case Manager and Community Outreach Nurse for a Healthcare for the Homeless grantee clinic in Fargo, the author is the only nurse outside of a traditional environment who works with victims of trafficking in the largest metropolitan area of North Dakota. The majority of the current targets for this heinous industry are young Native American women. The author, a Lakota woman, employs an approach with trafficking victims that seeks to reestablish the view of self as a being with significant value and ability to contribute to the world in a way that no other being can. In advocacy, she teaches professionals about the Lakota view of the Earth as a living being whose destruction may be correlated with the increased violence against women.
#Nursing Now
Authors: Stilwell Barbara
Published: February 2019

Nurses are the largest group in the global health workforce, but due to social, political, and gender inequality, traditionally their role has been undervalued. Nursing Now is a 3-year campaign which aims to empower nurses worldwide by building grassroots support to demand better investment in nursing and midwifery to tackle 21st-century health challenges.
Değişik Zamanlarda Hasat Edilen Farklı Tipteki Şeker Pancarı (Beta vulgaris var. saccharifera L.) Çeşitlerinin Verim ve Bazı Kalite Özelliklerinin Belirlenmesi
Authors: Tosun Bekir , Karadoğan Tahsin , Şanlı Arif
Published: February 2019

Isparta
ekolojik koşullarında 2012 ve 2013 yıllarında yürütülen bu araştırmada farklı
zamanlarda hasat edilen (çıkıştan 140, 155, 170, 185 ve 200 gün sonra) değişik
tiplerdeki şeker pancarı çeşitlerinin (Begonia, Aranka ve Esperanza (N tipi),
Sandrina ve Esperia (NZ tipi), Felicita ve Agnessa (Z tipi) verim ve bazı
kalite özellikleri incelenmiştir. En yüksek kök gövde ve biyolojik verim erken
sökümde Aranka, geç sökümde ise Esperia çeşitlerinden elde edilmiştir. En düşük
α-amino
azot miktarı erken sökümde Aranka (5.68 mmol/100g), geç sökümde ise Esperia
(2.05 mmol/100g) ve Esperanza (2.11 mmol/100g) çeşitlerinde belirlenmiştir. Erken
ve geç sökümlerde Z tipi olan Felicita ve Agnessa çeşitleri en yüksek polar
şeker oranına sahip olmuştur.  En yüksek
polar şeker verimi erken sökümde Aranka (1490 kg/da), geç sökümde ise Esperia
(1867 kg/da) ve Sandrina (1860 kg/da) çeşitlerinden elde edilmiştir. Çalışmada,
Isparta koşullarında yüksek verim ve teknolojik olgunluk bakımından Aranka ve
Begonia çeşitlerinin 200 gün, diğer çeşitlerin ise en az 185 günlük vejetasyon
dönemini tamamlaması gerektiği sonucuna varılmıştır.
Tarımsal Atıktan Elde Edilen Aktif Karbon Destekli Co-B Katalizörü Varlığında Sodyum Borhidrürün Metanolizi
Authors: KAYA Mustafa , BEKİROĞULLARI Mesut
Published: February 2019

Bu
çalışmada, tarımsal atık olan Siirt fıstığı kabuğundan elde edilen aktif karbon
kullanılarak destekli Co-B katalizörü sentezlenmiş ve bu katalizör hidrojen
eldesinde kullanılmıştır. Siirt yöresinin en önemli tarımsal gelir
kaynaklarının başında Siirt fıstığı gelmektedir. Fıstık işlendikten sonra
geriye kalan fıstık kabuğu ise tarımsal atık olarak kabul edilmekte olup, bu
atığın etkin bir şekilde kullanılması ise oldukça önem arz etmektedir. Aktif
karbon destekli Co-B katalizörü, sodyum
borhidrür (NaBH4)’ün metanol
ortamındaki bozunma reaksiyonu etkinliklerinde kullanılmış olup, farklı NaBH4
konsantrasyonları, farklı katalizör miktarları ve farklı sıcaklıklar
varlığında deneyler yapılarak etkinlikleri incelenmiştir. Çalışmada; 30 °C
sıcaklıktaki aynı NaBH4 konsantrasyonun, farklı katalizör miktarları
kullanılarak yapılan metanoliz deneylerinde, katalizör miktarı arttıkça
reaksiyonun tamamlanma süresi kısalmakta ve hidrojen üretim hızının artmakta
olduğu gözlemlenmiştir. Aktif karbon destekli Co-B katalizörü varlığında
metanoliz deneylerinin farklı sıcaklık (30-60 oC) etkinlikleri
incelendiği zaman ise sıcaklığın artmasıyla reaksiyon hızındaki artışın da
belirgin bir şekilde olduğu görülmüştür. Bunun yanı sıra tarımsal atıkların
işlenerek katalizörlerde destek maddesi olarak kullanımının katalizör
etkinliğini arttırdığı tespit edilmiştir. Son olarak; bu deney sonuçlarından
elde edilen veriler kullanılarak reaksiyon modeli çıkarılmış olup, buna bağlı
olarak bir ampirik formül türetilmiş ve bundan sonra yapılacak deneylerin
sonucunu teorik olarak da elde edilebilecek denkleme ulaşılmıştır. Yapılan bu
çalışma sayesinde tarımsal atık olarak kabul edilen Siirt fıstığı kabuğu etkin
bir şekilde değerlendirilmiş olup, bu atık malzeme katma değeri yüksek olan bir
ürüne dönüştürülmüştür.
“I Belong to Nowhere”: Syrian Refugee Children’s Perspectives on School Integration
Authors: Guo Yan , Maitra Srabani , Guo Shibao
Published: February 2019

Since 2011, the armed conflict that began in the Syrian Arab Republic has displaced an estimated 12 million Syrians, forcing them to seek refuge in various countries around the world. Over half of those uprooted are children. Education is key to integration of refugee children and is considered critical in bringing back a sense of normalcy, routine as well as emotional and social well-being in the lives of refugee children. In Canada, integration of Syrian refugee children in the public school system has, therefore, been identified as one of the vital aspects of their settlement needs. This article examines the challenges experienced by newly arrived Syrian refugee children as they struggle to integrate to the Canadian school system. We have conducted five focus groups with twelve Syrian refugee parents and eighteen Syrian refugee children between the age group of 10-14. Our research shows that Syrian refugee children not only find it difficult to make friends with local students but are also subjected to constant bullying and racism that affect their sense of belonging and connection. Making the views of these students explicit, we hope to provide a starting point for not only understanding their experiences in more detail, but also for developing educational strategies, resources and policies that might best meet the needs of these students and future refugee children and youth.
Refugee Student Integration: A Focus on Settlement, Education, and Psychosocial Support
Authors: Stewart Jan , El Chaar Dania , McCluskey Kari , Borgardt Kirby
Published: February 2019

The rapid response to settle Syrian refugees in Canada has had a profound effect on communities, schools, and social service agencies. This article discusses a research program that examined the integration and settlement of Syrian children and youth in Winnipeg and Calgary. Through the examination of the school and community contexts, the research focused on the educational and psychosocial needs of re-settled Syrian refugees and the reciprocal learning between refugee, immigrant and Canadian-born students. With contributions from youth, parents, and relevant stakeholders, the research identified gaps in programming and services as well as promising practices that support newcomers. Issues surrounding trauma, interrupted schooling, separation and loss, racism and discrimination complicated the settlement and integration efforts. Findings indicate that Canadians and Canadian service providers have a major role in supporting the successful integration of refugees. Cultural support workers, cultural brokers, and community liaison personnel are paramount to bridging the school to families and community agencies.
In-flight Catering Service and Food Safety: Implementation of Hazard Analysis and Critical Control Point System in PT Aerofood ACS Surabaya
Authors: Kharisma Ayu Diah Mutiara
Published: February 2019

Hazard Analysis Critical Control Point (HACCP) is a system of quality assurance and food safety in preventing the emergence of problems ranging from materials, processes, and products. HACCP is a form of risk management system that is developed to ensure food safety with a preventive approach. This system is adopted to provide food safety assurance in food production process for consumers. The in-flight catering company service observed in this study is PT. Aerofood ACS Surabaya. The implementation of HACCP system in every production process has been applied by this company. However, there is still limited study to explore the implementation of the system in the company and identify any potential impediment of the system. This research is descriptive with observation method on production process of chicken oyster sauce menu. The control points used in the process of chicken oyster sauce production is by controlling the temperature upon receiving raw materials, storage system, cooking process, blast chilling, until portioning. One out of the ten of HACCP implementation stages, monitoring activity was found has not met SNI 01-4852-1998 requirement standard. Monitoring process was not implemented while checking the core temperature of the food during the cooking process. Nine other of HACCP implementations stages including of HACCP team formation, product description, drafting flow chart, hazard identification, critical conrol point determination, critical boundary determination, correction action, verification, and documentation were already in accordance with SNI requirement standard.
Physical Environmental Factors and Its Association with the Existence of Mycobacterium Tuberculosis: A Study in The Working Region of Perak Timur Public Health Center
Authors: Lestari Muslimah Diah Dwi
Published: February 2019

In 2017, Surabaya District Health Office reported an increasing number of new cases of pulmonary tuberculosis in the working region of of East Perak Surabaya Public Health Center (Puskesmas) and was reported as the highest rates of tuberculosis cases in Surabaya. Pulmonary tuberculosis can be transmitted through ambient air contamination initiated by the presence of Mycobacterium tuberculosis. This study aims to determine the association of physical environmental factors (temperature, humidity, lighting, ventilations’ size, floor materials, walls, ceilings, and density) in the presence of Mycobacterium tuberculosis in the air. This research is an observational analytic study with case control study design. The study sample consisted of 21 households/realtives of patients diagnosed with Pulmonary TB positive. The data was obtained from completed questionnaires by respondents, direct observation and laboratory examination of the presence of Mycobacterium tuberculosis in the air. Data analysis was conducted by Chi-square test supported by Fisher's Exact Test test. The results showed that the significant association between physical environment variables of temperature variables (p-value= 0,000), humidity (p-value= 0,000), lighting (p = 0,000), with the existence of mycobacterium tuberculosis inside of the room. While insignificant association was detected for the variables of ventilation (p = 0.397), floor materials (p = 0.229), the walls materials (p = 0.338), the ceiling condition (p = 0.331), and the dwellers density (p = 0.611), with the presence of Mycobacterium tuberculosis in the air of the common room this current study concludes that there is a significant association between temperature, humidity, and illumination with the presence of Mycobacterium tuberculosis in ambient of common room. Transmission of tuberculosis can be eliminated by using face masks, Despite the previous satisfied level of knowledge, the family behavior and practices when in contact with patients were insufficient to prevent pulmonary TB transmission.
Effect of bone marrow mesenchymal stem cells transplantation on BMP-15 expression and Graafian follicle count in mice model of endometriosis
Authors: Parasti Ratriana Via , Widjiati Widjiati , Dwiningsih Sri Ratna
Published: February 2019

Objectives: To determine the effect of bone marrow mesenchy-mal stem cells (BMSCs) on BMP-15 expression and Graafian follicle count in endometriosis mice.Material and Methods: This study was a laboratory randomized clinical trial on Mus musculus. The object of the study was 42 mice which were divided into 3 groups, the control, endome-triosis, and endometriosis + BMSCs groups. Comparison of BMP-15 expression and Graafian follicle count between groups was evaluated.Results: Immunohistochemical analysis showed that BMP-15 expression in control, endometriosis, and endometriosis + BMSCs groups had p=0.551, p=0.446 and p=0.917 with ANOVA test p=0.273, indicating no statistically significant differences between groups . Graafian follicular count in the three groups had p=0.31, p=0.001, and p=0.006, with the Kruskal-Wallis test p=0.001. Graafian follicles in the endometriosis + BMSCs group were higher than those in control and endometriosis groups.Conclusion:In the endometriosis mouse model with bone mar-row stem cell transplantation the BMP-15 expression in each group did not show a difference, but a significant difference was found in the number of Graafian follicles.
Ovarian Malignant Brenner Tumor
Authors: Alfianto Romi , Rahniayu Alphania , Yuliati Indra
Published: February 2019

Objectives: To report one malignant Brenner tumor (MBT) case in Dr. Soetomo Hospital, Surabaya, Indonesia, that underwent therapy from August 2016 to August 2017.Case report: A woman aged 62 years underwent total abdominal hysterectomy and bilateral salphingo-oophorectomy surgery with histopathologic results of malignant Brenner Tumor. From immunohistochemical examination the expression of p63 was positive. The patient had been treated with Paclitaxel carboplatin chemotherapy for 5-cycles, with the results of the last post-chemotherapy evaluation showing no recidive mass.Conclusion:There was only one case of malignant ovarian Brenner Tumor in Dr Soetomo Hospital, Surabaya, Indonesia, in 2014- 2016. The diagnosis can only be based on anatomic pathology examination. The specific tumor markers for malignant Brenner tumors have so far not been found.
Developing Cultural Competence as Part of Nursing Studies: Language, Customs and Health Issues
Authors: Khatib Mohammad , Hadid Salam
Published: February 2019

Introduction: Developing nurses' cultural competence begins with their basic training, and requires them to participate in an array of activities which raise their awareness and stimulate their interest, desire and curiosity to know about different cultures. The aim of this work is to evaluate a cultural competence teaching model for nursing students. Method: A qualitative and quantitative evaluation was done using a semi-structured questionnaire completed by 155 students. Results: An improvement in cultural awareness, knowledge and attitudes among students as well as their willingness to recognize the other's difference was noted. The qualitative evaluation raised 3 themes: attitude change, cultural intelligence improvement and exploring cultural similarities. Conclusions: Developing nurses' cultural competence needs to be part of their basic training and based on cultural knowledge and experiential learning methods as well as providing them the opportunity to be exposed to different cultures.
Pengaruh Konsentrasi Fosfat Terhadap Perbandingan Ca/P Hidroksiapatit dari Limbah Gipsum Industri Keramik
Authors: Kurniawan Asep Mufti , Hartini Sri , Cahyanti Margareta Novian
Published: February 2019

Gypsum waste has the potential to be made into hydroxyapatite (HAp). In this study synthesis of HAp with hydrothermal  method using microwave at heating temperature of 100 ºC was carried out. The purpose of this study was to obtain hydroxyapatite bioceramic compounds from gypsum waste, determine the ratio of Ca/P in obtained HAp and characterizing the obtained HAp. HAp characterization has been carried out using FTIR and XRD. The result showed that 2,5 grams of gypsum powder produced HAp powder yield of 65,61% to 84,43%. Calcium levels are 0,02440 % to 0,03466 %, while phosphate levels are 0,038 % to 0,055 %. The Ca/P ratio are 0,28 to 0,39. FTIR HAp characterization results indicate the presence of phosphate groups (PO43-), hydroxyl groups (OH-), and Ca-O groups. But based on XRD analysis, The HAp obtained is not a standard HAp.
Diagnosing Heart Disease using Wavelet Transformation and Adaptive Neuro Fuzzy Inference System (ANFIS) Based on Electrocardiagram (ECG)
Authors: Puspita Indah , Abadi Agus Maman
Published: February 2019

Heart disease is the leading cause of death in the world. Heart disease is called the silent killer, because it often occcurs suddenly. Therefore, periodic cardiac examination is very necessary to reduce cases of death from heart disease.Heart disease can be known through electrocardiogram (ECG) examination. This study aims to explain the process of diagnosing heart disease through ECG using wavelet transformation and Adaptive Neuro Fuzzy Inference System (ANFIS).The process of diagnosing heart disease begins with cutting ECG signal consisting of 9-11 waves into one ECG wave, then decomposition and extraction are performed using wavelet transformation to obtain 6 parameters. The parameters will be used as input in ANFIS model. Data obtained from ECG extraction are divided into 70% training data and 30% testing data The output from the ANFIS model is a diagnosis of heart diseases, such as left bundle branch block (LBBB),  right bundle branch block (RBBB), and normal. ANFIS learning is divided into 6 stages, namely clustering data with Fuzzy C-Means method, computing the degree of membership of each data, determining fixed neurons, looking for normalized firing strength, calculating the consequent parameter values, and determining network output.The results of the study obtained the best ANFIS model with 10 clusters. The level of accuracy, specificity, and sensitivity for training data is 100%, 100%, and 100%, respectively and for the testing data, the level of accuracy, specificity, and sensitivity is 100%, 100%, and 100%, respectively.
Behavioral and Neural Aspects of the Spatial Processing of Conspecifics in the Electrosensory System
Authors: Milam Oak , Ramachandra Keshav L. , Marsat Gary
Published: February 2019

Localizing the source of a signal is often as important as deciphering the signal’s message. Localization mechanisms must cope with the challenges of representing the spatial information of weak, noisy signals. Comparing these strategies across modalities and model systems allows a broader understanding of the general principles shaping spatial processing. In this review we focus on the electrosensory system of knifefish and provide an overview of our current understanding of spatial processing in this system, in particular, localization of conspecific signals. We argue that many mechanisms observed in other sensory systems, such as the visual or auditory systems, have comparable implementations in the electrosensory system. Our review therefore describes a field of research with unique opportunities to provide new insights into the principles underlying spatial processing.
Structural Connections between Long Genetic and Literary Texts
Authors: Petoukhov Sergey
Published: February 2019

Impressing discoveries in the field of the genetic code have been described by its researchers by means of the terminology borrowed from linguistics and the theory of communications. Leading experts on structural linguistics believe for a long time already that languages of human dialogue were formed not from an empty place, but they are continuation of genetic language or, anyhow, are closely connected with it, confirming the idea of information commonality of organisms. The aticle continues the theme about a connection of linquistic languages with the genetic language. It describes results of comparative study of long Russian literary texts (novels by L.Tolstoy, F.Dostoevsky, A.Pushkin, etc.) and long sequences of hydrogen bonds in double helixes of DNA of different organisms. Formalisms of quantum informatics are used in modeling some of these results taking into account thoughts of many researches about possible using principles of quantum informatics in organisation of living bodies.