bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Probability and Influencing Factors of Stroke in Followed-up Hypertension Patients
Authors: Li An-le , Qi Ying , Zhu Shuai , Hu Zhi-hao , Xu Xue-jin , Wang Ya-wei , Qian Xin-zhi
Published: July 2021

Objective

To explore the risk probability and influencing factors of stroke in followed-up hypertension (HP) patients through the analysis of long-term followed-up cohort data.
Methods

The method of followed-up observation cohort was used to collect the information of 168417 hypertension patients from 2002 to 2020 in Jiading district in Shanghai. Kaplan-Meier method was used to analyze the risk probability of stroke complications in long-term followed-up HP patients, and the influencing factors were analyzed by Cox proportional risk model.
Results

Among 168417 hypertension patients, 11143 cases had stroke, and the cumulative incidence rate was 6.62%, the male was 6.87% and the female was 6.37%. With the extension of the observation of patients, the cumulative risk probability of stroke in HP patients would continue to increasing, and the interval was not equidistant. The total cumulative risk probability of stroke in HP patients was 78.9%, and male was 91.0%, female was 70.7%. During the whole observation, the risk probability of stroke was not fixed, but fluctuates. There were 4 peak onset periods, which were 8 years (peak period 4.2%), 15 years (peak period 14.0%), 22 years (peak period 6.0%) and 26 years (peak period 13.9%). The highest risk probability of male patients was in 26 years (peak period 23.1%), and the second peak was in 15 years (peak period 15.6%). The highest risk probability of female patients was in 15 years (peak period 12.9%), and the second peak was in 26 years (peak period 8.7%). The risk probability of different sex, BP grad and BMI was different, male was higher than female, grade 3 HP higher than grade 1 and grad 2 HP, thin higher than normal weight. The major influencing factors of stroke complications were age (RR = 2.917, p < 0.001), body mass index (RR = 1.450, p < 0.001), family history of stroke (RR = 1.386, p < 0.001), blood pressure grad (RR = 1.148, p < 0.001), registry age (RR = 1.071, P < 0.001 and family history of hypertension (RR = 1.051, P < 0.001).
Conclusion

The risk probability of stroke among hypertension patients would continue to disproportionately increase during observation, and the total cumulative risk probability could finally reach 78.9%. Male was higher than female. Age, BMI, family history (stroke and HP), blood pressure grad and duration of HP were related to the occurrence of stroke complications.
Sodium Tanshinone IIA Sulfonate Improves Cognitive Impairment via Regulating Aβ Transportation in AD Transgenic Mouse Model
Authors: zhang shijie , Ma Hui-Han , Wan Can , Zhang Lu-Ding , Zhang Rong-Rong , Peng Dong , Qiao Li-Jun , Cai Ye-Feng , Huang Hong-Qiang
Published: July 2021

Alzheimer’s disease (AD) is a most common neurodegenerative disease. Sodium Tanshinone IIA Sulfonate (STS) has been reported to ameliorate AD pathology. However, the underlying mechanism is still unclear. In this study, APP/PS1 mouse model was used to explore the potential mechanism of STS against AD. Morris water maze and Y-maze tests showed that administration of STS (10 or 20 mg/kg/day) improved learning and memory abilities of APP/PS1 mice. STS reduced the levels of ROS and MDA, while improved the activity of SOD in both hippocampus and cortex in APP/PS1 mice. STS inhibited the activity of AChE, while improved the activity of ChAT in APP/PS1 mice. In addition, STS elevated the protein expressions of neurotrophic factors (BDNF and NGF) and synapse-related proteins (PSD93, PSD95 and SYP) in both the hippocampus and cortex in APP/PS1 mice. At last, STS improved the protein expressions of GLUT1 and LRP1. These results indicated that the potential mechanism of STS on AD might be related to Aβ transportation function via GLUT1/LRP1 pathway.
The Impact of Breastfeeding on Neurocognitive Outcome at Twelve Years – Secondary Analysis of a Prospective Cohort Study
Authors: Reilly Kelly , McConnell Barbara , Thompson Andrew , Hepper Peter , Stewart Moira , Shields Michael D , Mone Fionnuala
Published: July 2021

BackgroundThe objectives of this secondary analysis were to compare the; (i) cognitive and (ii) behavioural outcomes in children born congenitally normal, non-preterm, and non-growth restricted that were breastfed beyond one month of age with those that were not breastfed or breastfed for <1-month. MethodsAn original prospective cohort study conducted on n=2097 fetuses compared children with normal fetal UAD PI (Umbilical Artery Doppler Pulsatility Index) against those with an elevated UAD PI and assessed neurocognitive development at twelve years of age using British Ability Scale Version II (BAS II) and the Achenbach Behavioural Checklist Parent Rated Version (CBCL). This secondary analysis included children where maternal breastfeeding status was known (n=252). Childhood cognitive and behavioural scores were analysed and compared for the breastfed versus the non-breastfed group. Linear regression analysis was performed, controlling for confounders including gender, age at assessment, Townsend Score, and UAD PI. Results Excluding congenitally abnormal, preterm and growth restricted babies, n=206 were included. The mean age at follow-up was 12.2 years (±0.6 SD) for both groups with 43.5% (n=20) and 50.3% (n=81) of male gender in both groups respectively. When comparing those children who were breastfed against those who were not or for <1-month, the breastfed group score higher in spelling (p<0.001) and reading (p<0.001) assessments. As was also the case in verbal (p<0.001), reasoning (p<0.001) and spatial ability scores (p<0.001). In relation to behavioural assessments, there was no difference between groups. Conclusion Term babies who are breastfed for >1-month have more optimal scores in neurocognition when analysed at 12-years with no differences in parent observed behaviour compared to non-breastfed/breastfed for <1-month counterparts. These findings must be interpreted with caution due to the presence of confounders with further research into the biological and physiological mechanisms by which breastfeeding may optimise brain development.
The GmXTH1 Gene Improves Drought Stress Resistance of Soybean Seedlings
Authors: Zhang Ye , Zhang Han-zhu , Fu Jia-yu , Du Ye-yao , Qu Jing , Song Yang , Wang Pi-wu
Published: July 2021

To study the role of GmXTH1 gene in alleviating drought stress. T4 transgenic soybean seeds with GmXTH1 gene were treated with PEG6000 at 0%, 5%, 10% and 15%, respectively. Germination potential, germination rate and germination index were measured. The results showed that the germination potential, germination rate and germination index of OEA1 and OEA2 strains overexpressed in T4 generation were significantly higher than that of control material M18. After 0d, 7d and 15d drought stress, the analysis of seedling phenotypes and root-shoot of different T4 generation transgenic soybean lines showed that under stress conditions, the growth of GmXTH1 overexpression material was generally better than that of control material M18, and the growth of GmXTH1 interference expression material was generally worse than that of control material M18, with significant differences in plant phenotypes.The root system of GmXTH1 overexpressed material was significantly developed compared with that of control material M18. The analysis of physiological and biochemical indexes showed that the relative water content and the activity of antioxidant enzymes (superoxide dismutase and peroxidase) of GmXTH1 transgenic soybean material were significantly higher than that of control material M18, and the accumulation of malondialdehyde was lower under the same stress conditions at seedling stage. Fluorescence quantitative PCR assay showed that the relative expression of GmXTH1 gene in transgenic soybean was significantly increased after drought stress.The results showed that the overexpression of GmXTH1 could increase the total root length, surface area, total projection area, root volume, average diameter, total cross number and total root tip number, thereby increasing the water intake and reducing the transpiration of water content in leaves, thus reducing the accumulation of MDA and producing more protective enzymes in a more effective and prompt way.Reducing cell membrane damage to improve drought resistance of soybean.
Experimental study on creep characteristics of infiltrated coal-rock under load
Authors: Zhang Huimei , wang fuyu
Published: July 2021

For the water-rich zone in the coal tunnel rock body perennial suffer from water infiltration destabilization destruction problem.In this paper, the influence of stress level and infiltration time on the creep properties of coal-rock was systematically studied after the water infiltration test, scanning electron microscope(SEM), water content test, uniaxial compressive strength test and creep mechanical properties test.The whole process of coal-rock creep is described by Hooke's body, Kelvin body and damage elastic-plastic body, and the total damage variable was introduced to show the weakening effect of the coupling effect of stress and water infiltration on the creep properties of coal-rock.A creep constitutive model of coal rock damage considering the weakening effect of water infiltration was established, besides, the influence law of stress level and infiltration time on creep parameters of coal rock was analyzed.The study shows that coal rocks underwent a process from surface drying and shrinkage to water absorption and swelling to water-filled infiltration damage in the infiltration test.With the increase of infiltration time, the water content of coal-rock tended to increase and eventually stabilizes, while the uniaxial compressive strength gradually decreased.With the increase of stress level and infiltration time, the stable creep strain of coal-rock kept increasing which accelerated creep advance, and its internal damage continued to accumulate and eventually led to destabilization damage.At the same stress level, the creep parameter E0 showed a tendency to increase and then decreased with the increase of infiltration time, while E1, η1, tF, E2 and ν continued to decrease.Combined with the microstructural changes of coal rocks in the water infiltration process, the change law of mechanical properties of infiltrated coal-rock and the intrinsic softening mechanism were revealed.Comparing the theoretical model of infiltrated coal rock creep with the experimental data, the model developed in this paper reflected the whole process of infiltrated coal-rock creep deformation and damage, and can characterize the influence of infiltration time and stress level on coal-rock creep properties, which verified the reasonableness of the model.
A Single Administration of Riluzole Applied Acutely After Spinal Cord Injury Attenuates Pro-Inflammatory Activity And Improves Long-Term Functional Recovery In Rats
Authors: Wu Qichao , Zhang Wenkai , Yuan Shuo , Zhang Yanjun , Zhang Wenxiu , Zhang Yan , Chen Xueming , Zang Lei
Published: July 2021

After spinal cord injury (SCI), emergency treatment intervention can minimize tissue damage, which is closely related to the recovery of long-term function. Here, we examined whether the administration of a single dose of riluzole (6 mg/kg) immediately after SCI was a critical window for the drug to exert its regulatory effect and limit long-term neurological deficits. The animals were sacrificed 1 day after administration for investigation of neuronal survival and a potential neuroinflammatory response, and sacrificed in the 6th week for assessment of neurological function. Riluzole applied in a single dose immediately post-SCI decreased the mRNA level of interleukin-1β at 6 h, reduced the destruction of neurons, and reduced the activation of microglia/macrophage M1 expression at day 1 post-SCI. Additionally, riluzole-treated rats showed higher expressions of interleukin-33 and its receptor ST2 in microglia/macrophages of the spinal cord than vehicle-treated rats, suggesting that this signaling pathway might be involved in microglia/macrophage-mediated inflammation. At 6 weeks, riluzole-treated rats exhibited higher motor function scores than vehicle-treated controls. In addition, riluzole-treated rats exhibited higher expression of GAP43 protein and shorter N1 peak latency and larger N1-P1 amplitude in motor-evoked potentials, compared to vehicle-treated rats. Together, these data suggested that early application of riluzole after SCI could be crucial for long-term functional recovery, so it may represent a promising therapeutic candidate within the critical therapeutic window for acute SCI.
Sleep Disturbance in Cancer Patients Referred to an Ambulatory Integrative Oncology Consultation
Authors: Narayanan Santhosshi , Reddy Akhila , Lopez Gabriel , Liu Wenli , Ali Sara , Bruera Eduardo , Cohen Lorenzo , Yennurajalingam Sriram
Published: July 2021

Background
Sleep disturbance (SD) is highly prevalent in oncology and negatively affects quality of life and mortality. Evidence supports the use of integrative oncology (IO) practices to treat SD, but there is limited published data on the characteristics of SD and factors associated with SD in IO. We determined the prevalence, severity, and factors associated with SD.
Methods
Patients with cancer referred for initial outpatient IO consultation in 2017 were eligible. Patient demographics, clinical characteristics, and patient-reported outcomes [Edmonton Symptom Assessment Scale, (ESAS), Measure Yourself Concerns and Wellbeing (MYCaW), PROMIS-10] were retrospectively reviewed.
Results
1520 patients were included in the analysis. The majority (70%) were women with breast cancer (42%). 971 (64%) patients reported significant SD with ESAS Sleep ≥ 4, yet only 11% expressed poor sleep as their primary or secondary concern for the IO consultation. The median SD (IQR) was 5 (3,7). ESAS scores for fatigue (adjusted OR 1.16; CI 1.07–1.26, p < 0.001), pain (adjusted OR 1.07; CI 1.00-1.15, p < 0.05), hot flashes (adjusted OR 1.14; CI 1.07–1.22, p < 0.001), well-being (adjusted OR 1.33; CI 1.22–1.46, p < 0.001), and psychological distress score (anxiety and depression) (adjusted OR 1.16; CI 1.01–1.11, P < 0.01) were independently associated with SD in multivariate analysis. Acupuncture was the most frequent intervention prescribed 175 (35%). Other modalities included oncology massage (15%), health psychology (5%) and meditation (1%).
Conclusions
Although 64% of patients seeking IO consultation reported clinically significant SD, only 11% were seeking integrative approaches for managing SD. ESAS fatigue, hot flashes, well-being, and psychological symptoms were significantly associated with SD.
Oral prodrug of remdesivir parent GS-441524 is efficacious against SARS-CoV-2 and a variant of concern in ferrets
Authors: Plemper Richard , Cox Robert , Wolf Josef , Lieber Carolin , Sourimant Julien , Lin Michelle , Babusis Darius , Du Pont Venice , Chan Julie , Barrett Kim , Kalla Rao , Chun Kwon , Mackman Richard , Ye Chengjin , Cihlar Tomas , Martinez-Sobrido Luis , Greninger Alexander , Bilello John
Published: July 2021

Remdesivir is the only small-molecule antiviral approved to date for COVID-19 treatment, but its wider use is limited by intravenous delivery. An orally bioavailable remdesivir analog may boost therapeutic benefit by facilitating early administration to non-hospitalized patients. This study characterized the anti-SARS-CoV-2 efficacy of GS-621763, an oral prodrug of remdesivir parent nucleoside GS-441524. Both GS-621763 and GS-441524 inhibited SARS-CoV-2, including variants of concern (VoC) in cell culture. Oral GS-621763 was efficiently converted to plasma metabolite GS-441524, and in lungs to the triphosphate metabolite identical to that generated by remdesivir, demonstrating a consistent mechanism of activity. Twice-daily oral administration of 10 mg/kg GS-621763 reduced SARS-CoV-2 burden to near-undetectable levels. When dosed therapeutically against VoC P.1 gamma (γ), oral GS-621763 blocked virus replication and prevented transmission to untreated contact animals. These results demonstrate therapeutic efficacy of a much-needed orally bioavailable analog of remdesivir in a relevant animal model of SARS-CoV-2 infection.
Insight into the Water Rock Interaction Process and Purification Mechanism of Mine Water in Underground Reservoir of Daliuta Coal Mine in China
Authors: Jiang Binbin , Gao Ju , Du Kun , Deng Xu , Zhang Kai
Published: July 2021

The process of water rock interaction and the purification mechanism of mine water quality were not clear after being stored in underground reservoir. This study based on the analysis of the hydrochemical characteristics of the reservoir water samples and the characterization of the rock samples, combined with PHREEQC analysis, the mechanism of water quality purification of mine water was discussed. The results showed that the rocks in the underground reservoir had layered silicate structure and flaky kaolinite structure, with some irregular edges and micro cracks, and higher specific surface area and total pore volume. These characteristics made the rocks had a certain adsorption and removal capacity for heavy metal ions and other pollutants in the mine water. The water rock interaction, such as the dissolution of albite and halite, the precipitation of gypsum and kaolinite, and the cation exchange, resulted in the increase of the concentration of Na+ and the decrease of the concentration of Ca2+, Mg2+ and TDS in the outlet water. This study also showed that PHREEQC analysis can be used to analyze the water rock interaction of coal mine underground reservoir and obtained more detailed information.
Hydro-Geochemical Characteristics of Groundwater Uses for Agricultural & Drinking and Groundwater Quality of Pollution Index in the Western Part of Telangana, South India
Authors: EDUKONDAL ALLAM , Duvva Laxman Kumar , Ramu Malyala , HARIKRISHNA GANGULA , Muralidhar Merugu
Published: July 2021

Groundwater is a major valuable asset, which helps to sustain human ecology, particularly in crystalline terrains. Hence, proper quality monitoring of groundwater is a crucial task in understanding its suitability for drinking water and irrigation water practices. For this, fifty groundwater samples were taken and evaluated for physical, chemical characteristics and major ions during in pre and post-monsoon seasons. The results obtained were compared with IS 10500 standard which shows more than 80% of the samples in both seasons were unfit for drinking. The obtained results were checked to know the irrigation suitability. It is found that most of the samples (80%) were fit for agricultural practices in both seasons. The major water facies were CaCl, mixed CaMgCl and NaCl water types in pre and post-monsoon seasons. The spatial distribution diagrams of the major ions were high concentration in Northern areas followed by Western and South-Western portions. As per the groundwater quality of pollution index (GQPI), most of the study region (62 percent) comes under the low contamination zone and the rest (38 percent) under the moderate to unacceptable zone in drinking water specifications. Factor analysis reveals that the study region which predominant of weathering of silicate minerals, ion exchange and anthropogenic inputs of major contamination of groundwater quality.
Comparison of YOLO v3, Faster R-CNN, and SSD for Real-Time Pill Identification
Authors: Tan Lu , Huangfu Tianran , Wu Liyao , Chen Wenying
Published: July 2021

Background: The correct identification of pills is very important to ensure the safe administration of drugs to patients. We used three currently mainstream object detection models, respectively Faster R-CNN, Single Shot Multi-Box Detector (SSD), and You Only Look Once v3(YOLO v3), to identify pills and compare the associated performance.Methods: In this paper, we introduce the basic principles of three object detection models. We trained each algorithm on a pill image dataset and analyzed the performance of the three models to determine the best pill recognition model. Finally, these models are then used to detect difficult samples and compare the results.Results: The mean average precision (MAP) of Faster R-CNN reached 87.69% but YOLO v3 had a significant advantage in detection speed where the frames per second (FPS) was more than eight times than that of Faster R-CNN. This means that YOLO v3 can operate in real time with a high MAP of 80.17%. The YOLO v3 algorithm also performed better in the comparison of difficult sample detection results. In contrast, SSD did not achieve the highest score in terms of MAP or FPS.Conclusion: Our study shows that YOLO v3 has advantages in detection speed while maintaining certain MAP and thus can be applied for real-time pill identification in a hospital pharmacy environment.
Real-World Effectiveness of A Digital-Based Coaching Intervention for Cancer Survivors with Job Loss
Authors: Lo Jonathon , Ballurkar Kieran , Fox Simonie , Tynan kate , Luu Nghiep , Boyer Michael , Murali-Ganesh Raghav
Published: July 2021

Purpose:Return-to-work (RTW) is a key unmet need for working age cancer survivors. This study sought to evaluate RTW outcomes of a multidisciplinary intervention provided as routine employee support.Method:In a retrospective cohort analysis, patients with cancer and more than 3 months absent from work were provided an intervention consisting of digital resources and calls with a health coach. A logit regression model was used to calculate a propensity score using covariates of age, gender, insurance benefit type, date of cancer diagnosis and time from diagnosis derived from insurance-claims data and captured as standard business practice. Participants were matched on a 1:1 basis using the nearest-neighbor method without replacement to create a matched control group from a further 1,856 participants who did not receive the intervention.Results:220 participants enrolled in the intervention, of which 125 met the criteria for analysis. The median follow-up from cancer diagnosis was 79 weeks (IQR 60-106). In the matched control group, 22 returned to work (17.6%) compared with 38 (30.4%) in the intervention group (P=.02). Nineteen matched controls died prior to claim closure (15.2%) compared with 13 in the intervention group (10.4%; P=.26). Cox model estimated median time for the first 15% of the cohorts to RTW was 87.1 weeks for the matched control (CI 60.0-109.1 weeks) compared with 70.6 weeks for the intervention (CI 52.6-79.6 weeks; P=.08).Conclusion:A digitally delivered coaching program in a real-world setting for patients diagnosed with cancer improves the likelihood of RTW.Implications for cancer survivors: a remotely delivered coaching program in a real-world setting for cancer survivors can improve the likelihood of RTW.
Traditional Chinese Exercises for Chronic Diseases: Protocol for An Overview of Systematic Reviews and Meta-analyses
Authors: Zhang Fan , Bai Yan , Zhao Xing , Wang Hui , Zhou Wenqin , Zhang Huachun
Published: July 2021

Background: In recent years, increasing systematic reviews and meta-analyses about traditional Chinese exercises on chronic disease have been conducted. The main aim of this overview will be to appraise and synthesize these results. Methods A comprehensive review of the literature will be performed in the PubMed, Cochrane Database of Systematic Reviews, Embase, and Web of Science Core Collection, with the MeSH terms and keywords. Systematic reviews and meta-analyses of clinical trials evaluating the effectiveness of traditional Chinese exercises for patients with chronic disease will be included. Two independent authors will screen the titles and abstracts, and full-text articles will be identified. Relevant data will be extracted and tabulated. A Measurement Tool to Assess Systematic Reviews and the Grading of Recommendations Assessment, Development and Evaluation system will be used to assess the methodological quality and level of evidence of included reviews, respectively.Discussion: The overview will identify both the scientific and clinical importance of traditional Chinese exercises, and these findings will provide practical implications for patients, clinicians, professionals, and policymakers.Systematic review registration: PROSPERO CRD42021248444
Identification of Potential Signal Pathways and Immune Disorders in Crohn's Disease
Authors: zou qiyuan , Long Weiyan , Lv Xiaodan , Liu Gengfeng , Chen Ruyan , Zhan Lingling , Lv Xiaoping
Published: July 2021

Background: In recent years, the number of visits to Crohn's disease (CD) has gradually increased, but its pathogenesis is still unclear1.This research aimed to explore the potential molecular pathways and immune disorders of CD.Methods:The two gene datasets of GSE83448 and GSE95095 were used to identify differentially expressed genes (DEGs) in our study. We also carried out the function enrichment analyses of DEGs between the CD group and the control group.xCell is used to identify the types of lymphocytes in the microenvironment. Subsequently, the correlation analysis between potential enrichment pathways and lymphocyte infiltration in the microenvironment was performed. We mined 944 DEGs (321 up-regulated and 623 down-regulated) and 1467 DEGs (781 up-regulated and 686 down-regulated) in the GSE83448 and GSE95095 datasets, respectively. Results: Comprehensive analysis shows that: the Focal adhesion and ECM−receptor interaction are the main significant enrichment pathways. xCell analysis identified significantly altered immune cells in CD, Th17cells, included pDC, Th1cells, Blood vessels, Macrophages, NK CD56dim cells, Tem, NK cells, NK CD56bright cells, Lymph vessels. The enrichment scores of Focal adhesion and ECM-receptor interaction signaling pathways in the CD intestinal mucosa were significantly correlated with the abundance of immune cells in the two sets of data. Conclusions:These data suggest the important role of Focal adhesion and ECM-receptor interaction signal transduction pathways in CD, and the intimate relationship between these two pathways and specific types of immune cell infiltration.This study confirmed the potential molecular pathways and immune disorders in CD.
Risk Evaluation of Denosumab And Zoledronic Acid For Medication-Related Osteonecrosis of The Jaw In Patients With Bone Metastases: A Propensity Score-Matched Analysis
Authors: Ikesue Hiroaki , Doi Kohei , Morimoto Mayu , Hirabatake Masaki , Muroi Nobuyuki , Yamamoto Shinsuke , Takenobu Toshihiko , Hashida Tohru
Published: July 2021

Purpose: This study evaluated the risk of medication-related osteonecrosis of the jaw (MRONJ) in patients with cancer who received denosumab or zoledronic acid (ZA) for treating bone metastasis.Methods: The medical records of patients were retrospectively reviewed. Patients who did not undergo a dental examination at baseline were excluded. The primary endpoint was a comparison of the risk of developing MRONJ between the denosumab and ZA groups. Propensity score matching was used to control for baseline differences between patient characteristics and compare outcomes for both groups.Results: Among the 799 patients enrolled, 58 (7.3%) developed MRONJ. The incidence of MRONJ was significantly higher in the denosumab group than in the ZA group (9.6% [39/406] vs. 4.8% [19/393], p = 0.009). Multivariate Cox proportional hazards regression analysis revealed that denosumab treatment (hazard ratio [HR], 2.89; 95% confidence interval [CI], 1.65–5.25; p < 0.001) and tooth extraction after starting ZA or denosumab (HR, 4.26; 95% CI, 2.38–7.44; p < 0.001) were significant risk factors for MRONJ. Propensity score-matched analysis confirmed that the risk of developing MRONJ was significantly higher in the denosumab group than in the ZA group (HR, 2.34; 95% CI, 1.17–5.01; p = 0.016). Conclusion: The results of this study suggest that denosumab poses a significant risk for developing MRONJ in patients treated for bone metastasis, and thus these patients require close monitoring.
Combining Ability And Nature of Gene Effects Controlling Seed Yield And Its Component Traits In Alien Cytoplasm Based Hybrids In Sunflower (Helianthus Annuus L.)
Authors: Sharma Manish , Y.G.SHADAKSHARI
Published: July 2021

The present research aimed to study gene effects for seed yield and its component traits in sunflower hybrids having diverse cytosterile sources from Helianthus annuus and H. argophyllus. Five lines and ten testers were crossed in a line x tester mating design to develop 50 F1 hybrids for genetic analysis. The analysis of variance revealed significant differences among the traits studied. Seasonal variation was found significant for all the traits except stem diameter, volume weight and seed filling percentage. The mean squares of lines and testers from crosses both determine the GCA were also significant for most of the traits which revealed the prevalence of additive variances and additive gene action. The mean squares of lines x tester interactions were also significant for all the traits considered. The significance of lines x tester interactions indicated that SCA is also important in the expression of traits and demonstrated the value of non-additive variances and dominant genes controlling the various traits. Among the diverse cytosterile lines, ARG-6-3-1-4 was identified as the best general combiner for stem diameter, volume weight, seed yield, hull content, oil content and oil yield. While, the line ARG-2-1-2 was best general combiner for days to 50 per cent flowering, head diameter, 100 seed weight and seed filling percentage. The tester M17-R was observed to be best general combiner for earliness and volume weight, while RHA 93 was the best general combiner for plant height, seed yield and oil content. The best cross combinations; MUT-2-8-3-2 x GKVK 3 was found to be good specific combiner for stem diameter, 100 seed weight, seed yield, seed filling percentage, oil content and oil yield, while ARG-6-3-1-4 x GKVK 3 was good specific combiner for days to 50% flowering, plant height and 100 seed weight.
Sensing the lightness: a narrative analysis of an integrative medicine program for healthcare providers in the COVID-19 department
Authors: Ben-Arye Eran , Zohar Shaked , Keshet Yael , Gressel Orit , Samuels Noah , Eden Arieh , Vagedes Jan , Kassem Sameer
Published: July 2021

Objectives
There is limited research addressing physical and emotional exhaustion among healthcare providers (HCPs) in COVID-19 departments. We examined the impact of an integrative medicine (IM) intervention for HCPs working in isolated COVID-19 in-patient departments, addressing concerns and wellbeing.
Methods
HCPs working in 3 isolated COVID-19 in-patient departments underwent 40-minute IM treatment sessions (including acupuncture, manual-movement and/or mind-body modalities) provided by integrative oncology practitioners. The MYCAW (Measure Yourself Concerns and Well-being) questionnaire was used to address the two leading concerns and post-treatment reflections and narrative. Data were qualitatively analyzed using ATLAS.Ti software for systematic coding.
Results
A total of 181 HCPs underwent 305 IM treatments. Narrative themes focused on physical symptoms (primarily pain and fatigue) and emotional concerns, including perceived communication barriers with patients; and reflections on wellbeing and insights following IM treatments. HCPs reported feeling a sense of "relief" which was likely related to the 3 main effects of the IM intervention: a sense of "being cared for" and treated; experiencing emotional, sometimes spiritual effects of the treatment; and the feeling of relaxation, combined with the relief of pain. Qualitative analysis identified clusters of emotional and spiritual-related keywords such as "calming", "release", "relaxation” and “disengagement” following the first IM session (119 of 181 narratives, 65.7%).
Conclusions
HCPs working in isolated COVID-19 departments reported improved wellbeing and the addressing of their concerns following IM treatment sessions provided during their work shift. Further research is needed to explore the impact of IM on HCPs burnout and resilience in palliative care settings.
Seismic Behavior Investigations of the Haunched Joint with Ribbed Anchor Webs in CFDST Structures
Authors: zhang yufen , Bu Hongfan , Cao Shengxi
Published: July 2021

The haunched joint with ribbed anchor webs in the concrete-filled double steel tubular (CFDST) structure was proposed in this study. The CFDST column and the steel beam were connected by vertical ribbed webs, which were anchored inside the column and connected to the beam web by the bolted connection, and haunches were welded to shift plastic hinges from the joint core to beam ends. The cyclic loading test was conducted on three beam-to-column joint specimens. Seismic behaviors including failure modes, strain and stress responses, hysteretic curves, ductility, and energy dissipation capacity were respectively analyzed. Besides, a finite element (FE) modeling was completed by ABAQUS in consideration of geometric and material nonlinearity. The validity of FE modeling was checked by comparisons between FE results and test results, and research results confirmed that the beam-to-column rotation and the shear deformation in the joint core were small owing to the restriction from the configuration of haunches. Then parametric investigations on connection constructional details, geometric dimensions, material strength, and axial compression ratios, were made to study seismic behaviors of the haunched joint with ribbed anchor webs. It showed that the selected parameters except concrete strength had certain influences on bearing capacity, ductility, and energy dissipation, and corresponding practicable design suggestions were proposed for the application of haunched joints with ribbed anchor webs in CFDST structures.
Study of the Contribution of Active Defense Mechanisms to Ciprofloxacin Tolerance in Escherichia Coli Growing at Different Rates
Authors: Smirnova Galina , Tyulenev Aleksey V. , Muzyka Nadezda G. , Oktyabrsky Oleg N.
Published: July 2021

Using rpoS, tolC, ompF, and recA knockouts, we investigated their effect on the physiological response and lethality of ciprofloxacin in E. coli growing at different rates on glucose, succinate or acetate. We have shown that, regardless of the strain, the degree of changes in respiration, membrane potential, NAD/NADH ratio, ATP and glutathione (GSH) strongly depends on the initial growth rate and the degree of its inhibition. The deletion of the regulator of the general stress response RpoS, although it influenced the expression of antioxidant genes, did not significantly affect the tolerance to ciprofloxacin at all growth rates. The mutant lacking TolC, which is a component of many E. coli efflux pumps, showed the same sensitivity to ciprofloxacin as the parent. The absence of porin OmpF slowed down the entry of ciprofloxacin into cells, prolonged growth and shifted the optimal bactericidal concentration towards higher values. Deficiency of RecA, a regulator of the SOS response, dramatically altered the late phase of the SOS response (SOS-dependent cell death), preventing respiratory inhibition and a drop in membrane potential. The recA mutation inverted GSH fluxes across the membrane and abolished ciprofloxacin-induced H2S production. At all growth rates, this mutation reduced logCFU ml-1 by about 3 orders of magnitude. All studied mutants showed an inverse linear relationship between logCFU ml-1 and the specific growth rate, that is, the activity of antibiotic targets. Mutations shifted the plot of this dependence relative to the parental strain according to their significance for ciprofloxacin tolerance.
Influence of Local Mineral Raw Materials On The Commercial Success of Aveiro Production of Ancient Ceramic Sugar Jars
Authors: Fernando Rocha , Morgado Paulo
Published: July 2021

Sugar forms were conic ceramic jars having a hole at the bottom, being used specifically for the stage of the purge of the sugar cake. These pieces played a paramount role in sugar production cycle, being used for the maturation of the sugar, and since the 15th until the beginning of the 19th centuries, the old pottery centres from Aveiro and Lisbon regions, produced heavily these “formas de açúcar” (“sugar jars”) which were exported to sugar production areas, at places as diverse as Madeira, Canaries, Cape Verde, Cuba and Brazil. Mineralogical analysis by x-ray powder diffraction was carried out on bulk samples. Chemical composition was assessed by X-Ray fluorescence. The obtained results gave important information about the composition of the studied materials, and also about their raw materials. Mineralogical and chemical data obtained in samples from Aveiro point to a local production, using the upper Cretaceous (Maastrichian) marly (dolomitic) clays and clayey sands as main raw materials. Ceramics from Barreiro (Lisbon) are in general more silicated and less carbonated, composition close to the Tagus Cenozoic Basin clays. The higher iron content of Aveiro clays favours the glazing of ceramic paste at lower temperatures, giving better mechanical resistance which can justify "their best quality", as referred to in ancient documents.
Terminal Differentiation of Keratinocytes Was Damaged In Type 2 Diabetic Mice
Authors: Takayanagi Takeshi , Hirai Hiroyuki , Asada Yohei , Yamada Takaaki , Hasegawa Seiji , Tomatsu Eisuke , Maeda Yoshiteru , Yoshino Yasumasa , Hiratsuka Izumi , Sekiguchi-Ueda Sahoko , Shibata Megumi , Seino Yusuke , Sugimura Yoshihisa , Akamatsu Hirohiko , Itoh Mitsuyasu , Suzuki Atsushi
Published: July 2021

Aims: Although skin manifestations are common in diabetic patients, its characteristics are poorly identified. This study explored the differentiation process of keratinocytes in type 2 diabetes mellitus (T2DM) in vivo.Methods: Back skin of T2DM model KKAy/TaJcl mice (KKAy) and C57BL/6JJcl mice (control) aged 8 and 12 weeks was used. The mRNA expression of differentiation markers of keratinocytes was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The expression of each marker in situ was examined immunohistochemically.Results: KKAy mice showed hyperglycemia versus control mice. The histological findings showed increased thickness and structural impairment of epidermal tissue in KKAy mice. The qRT-PCR revealed that the expression of integrin beta 1 and keratin 14 in KKAy and control mice was identical. However, the expression of involucrin at 8 w, keratin 10 at 12 w, and filaggrin and loricrin at 8 and 12 w was decreased in KKAy mice. Immunohistochemical findings showed that filaggrin was markedly decreased in KKAy mice, though Ki-67 remained unchanged in control mice. Conclusion: The terminal differentiation process was impaired in the diabetic skin, while keratinocyte proliferation was preserved. Damaged terminal differentiation of keratinocytes may contribute to impairment of the skin barrier function in diabetic dermatoses.
The Impact of Surgeon Referral and a Radiation Oncologist Productivity-Based Metric on Radiotherapy Receipt Among Elderly Women with Early-Stage Breast Cancer: Analysis from a Tertiary Cancer Network
Authors: Patel Mira A , Cha Elaine E. , Lobaugh Stephanie , Zhang Zhigang , McCormick Beryl , Braunstein Lior Z. , Cahlon Oren , Powell Simon N. , Morrow Monica , Khan Atif , Gillespie Erin F.
Published: July 2021

Purpose: Guidelines for early-stage breast cancer allow for radiotherapy (RT) omission following breast conserving surgery (BCS) among older women, though high utilization of RT persists. This study explores surgeon referral and the effect of a productivity-based bonus metric for radiation oncologists in an academic institution with centralized quality assurance (QA) review.Methods: We evaluated patients >70 years of age treated with BCS for ER+ pT1N0 breast cancer at a single tertiary cancer network between 2015-2018. The primary outcomes were radiation oncology referral and RT receipt. Covariables included patient and physician characteristics, and treatment decisions before versus after productivity metric implementation. Univariable generalized linear effects models explored associations between these outcomes and covariables. Results: Of 703 patients included, 483 (69%) were referred to radiation oncology and 273 (39%) received RT (among those referred, 57% received RT). No difference in RT receipt pre- versus post- productivity metric implementation was observed (p=0.57). RT receipt was associated with younger patient age (70-74 years, OR 2.66, 95% CI 1.54-4.57) and higher grade (grade 3, OR 7.75, 95% CI 3.33-18.07). Initial referral was associated with younger age (70-74, OR 5.64, 95% CI 3.37-0.45) and higher performance status (KPS ³90, OR 5.34, 95% CI 2.63-10.83). Conclusion: Non-referral to radiation oncology accounted for half of RT omission, but was based on age and KPS, in accordance with guidelines. Lack of radiation oncologist practice change in response to misaligned financial incentives is reassuring, potentially reflecting centralized QA review. Multi-institutional studies are needed to confirm these findings.
Universe: the dynamics of the minimum and maximum expansion states
Authors: Petrakis Sócrates
Published: July 2021

Two hypotheses stand out in describing the evolution of the Universe. The predominant one predicts that the current expansion began at a certain instant and will not preserve any variation of energy that performs work; apparent flat Universe (Ω = 1) is advocated by relativistic calculations and observational data, with an end or thermal death at its maximum expansion (3D space). The other hypothesis considers that the Universe is always cyclical (alternating phases of expansion and contraction). This proposal aims to demonstrate that both hypotheses can be correct by not being distinct, but complementary. Supported by the immutability of physical laws, analyses of concepts such as space, mass, energy, gravity, spin, and entropy define an exclusive presence of 1D space in the minimum and maximum expansion states of the Universe. With our 3D space Universe created and existing between these extreme states, a complete universal evolution is outlined. More objectively, all the exposed dynamics complements the usual relativity. The concept of complete rest energy (1D space) was able to be applied, demonstrating that the evolution of the entire Universe is spatially dynamic in a perpetual time dimension, always recreating our Universe.
Deep Neural Network for the Determination of Transformed foci in Bhas 42 Cell Transformation Assay
Authors: Masumoto Minami , Fukuda Ittetsu , Furihata Suguru , Arai Takahiro , Kageyama Tatsuto , Ohmori Kiyomi , Sirakawa Shinichi , Fukuda Junji
Published: July 2021

Bhas 42 cell transformation assay (CTA) has been used to estimate the carcinogenic potential of chemicals by exposing Bhas 42 cells to carcinogenic stimuli to form colonies, referred to as transformed foci, on the confluent monolayer. Transformed foci are classified and quantified by trained experts using morphological criteria. Although the assay has been certified by international validation studies and issued as a guidance document by OECD, this classification procedure is laborious, time consuming, and subjective. We propose using deep neural network to classify foci more rapidly and objectively. To obtain datasets, Bhas 42 CTA was conducted with a potent tumor promotor, 12-O-tetradecanoylphorbol-13-acetate, and focus images were classified by experts (1405 images in total). The labeled focus images were augmented with random image processing and used to train a convolutional neural network (CNN). The trained CNN exhibited an area under the curve score of 0.95 on a test dataset significantly outperforming human-based classifiers by beginners of focus judgment. The generalization performance of unknown chemicals was assessed by applying CNN to other tumor promotors exhibiting an area under the curve score of 0.87. The CNN-based approach could support the assay for carcinogenicity as a fundamental tool in focus scoring.
Comprehensive Analysis of Genome-instability Related lncRNAs and Tumor-immune Microenvironment to Discriminate the Prognosis, Immunotherapy and PARP Inhibitor Responses in Patients with Low-grade Glioma
Authors: Zhou Zijian , Lu Bin , Wei JinHong , Guo Lei , He ZhongMing , Liu Yini , Wang Weimin , Li Luo
Published: July 2021

Background: Previous study revealed that Genome-instability was correlated with tumor-immune microenvironment in cancer. We try to discriminate the prognosis, immunotherapy and poly (ADP)-ribose polymerase (PARP) inhibitor responses through comprehensive analysis of genome-instability related lncRNAs and the tumor-immune microenvironment in patients with low-grade glioma (LGG). Methods and Results: RNAseq data, genome variation profiling data and copy number variation (CNV) data were used to evaluate the genomic instability of LGG patients. Genomic unstable-like (GU-like) and genomic stable-like (GS-like) clusters were identified by hierarchical clustering analysis of 102 genome-instability related lncRNAs (GILncRNAs). GS-like cluster had a tendency to receive better clinical outcome. Patients in GU-like cluster were more likely to respond to immunotherapy, especially anti-PD-1/PD-L1 treatment. PARP inhibitors including Rucaparib and Olaparib will get better therapeutic effects for patients in GU-like cluster. Lasso and Cox regression analysis were utilized to construct the risk model based on GILncRNAs. As for the risk model constructed by 9 GILncRNAs, the overall survival, clinical outcome, immunotherapeutic response, and PARP inhibitor sensitivity were significantly different between patients of high and low-risk groups. Conclusions: The genome-instability related lncRNAs signature involved in our risk model had great advantages in predicting prognosis, immunotherapy and PARP inhibitor response.
Creatinine Reduction Ratio is a Prognostic Factor for Acute Kidney Injury Following Cardiac Surgery with Cardio Pulmonary Bypass: A Single Center Retrospective Cohort Study.
Authors: anzai akiko , takaki shunsuke , Yokoyama Nobuyuki , Kashiwagi Shizuka , Yokose Masashi , Goto Takahisa
Published: July 2021

Background: Acute kidney injury (AKI) after cardiac surgery is common complication, and it is known as risk of death. In the previous study, we reported creatinine reduction ratio (CRR) can be useful as a prognostic factor of AKI. Primary outcome of this study was determination of predictor of AKI after surgery with using perioperative information. Secondary outcome was the reliability of CRR for short and long term outcome among the different nation.Methods: We retrospectively collected cardiac surgical patients with cardiopulmonary bypass from electrical health record. Patients were excluded with insufficient data. AKI was defined by the AKIN and RIFLE criteria, (1) increment of creatinine≧ 0.3mg/dl (2); increment of creatinine≧ 150%. Patients were divided by AKI and non-AKI group. We analyzed two group about the preoperative patients’ data and operative information. CRR was calculated as follows: (preoperative creatinine-postoperative creatinine)/preoperative creatinine. Results: 225 patients data were collected from electrical health record, and analysed. The prognostic factor of AKI-CS was surgery time, CPB time, aorta clamp time, platelet transfusion, and CRR < 20%. In the multivariable logistic analysis, CRR was an independent predictor of AKI. (adjusted odds ratio 0.90[0.87-0.93] p<0.001) However, there were not significant difference in CRR about the rate of new onset of chronic kidney disease (CKD). Conclusions: After cardiac surgery with cardiopulmonary bypass, the CRR has a good diagnostic power for predicting perioperative AKI. However, we cannot use it as a prognostic factor for long term period.