bluuebunny/crossref_metadata_2025 huggingface dataset:
Wild Thymus zygis L. ssp. gracilis and Eucalyptus camaldulensis Dehnh.: Chemical composition, antioxidant and antibacterial activities of essential oils
Authors: Aabouch Farah , Satrani Badr , Ameggouz Mouna , Ettaleb Ismail , Assouguem Amine , Kara Mohammed , Ullah Riaz , Bari Ahmed , Kaur Sawinder , Ghanmi Mohamed , Farah Abdellah , Ouajdi Mohamed , Annemer Saoussan , Dahmani Jamila
Published: June 2024

Natural substances extracted from plants have been increasingly studied and recognized, recently. Essential oils (EOs), for example, possess antioxidant and antibacterial properties, enabling their application across different sectors like agro-food, pharmaceuticals, and cosmetics. In Morocco, exceptional plant diversity, mirroring the diversity of ecosystems, has not yet revealed all its secrets. Therefore, the aim of this study is to determine the chemical composition and evaluate the antibacterial and antioxidant activities of EOs from Thymus zygis L. ssp. gracilis and Eucalyptus camaldulensis Dehnh. collected in the El Hoceima and Mamora regions, respectively. The EOs were extracted by hydrodistillation employing a Clevenger-type apparatus. Gas chromatography/mass spectrometry (GC/MS) analyses identified 54 constituents representing 92.65% of the total for T. zygis and 55 components representing 99.60% for E. camaldulensis. The primary components found in the EO of T. zygis are δ-terpineol (27.64%), followed by δ-3-carene (15.7%), thymol (14.17%), and dehydrolinalool (4.99%). The main compounds in E. camaldulensis EO are 1,8-cineole (43.61%), γ-terpinene (11.71%), α-terpineol (10.58%), and p-cymene (4.93%). The antioxidant properties of these oils were investigated by utilization of the 2,2-diphenyl-1-picrylhydrazyl method and the ferric reducing antioxidant power (FRAP) test. The antibacterial activity was assessed against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Micrococcus luteus and Escherichia coli). Both EOs showed significant antioxidant activity but were less effective than reference antioxidants quercetin and catechin. Antibacterial studies demonstrated strong activity of T. zygis and E. camaldulensis EOs against the studied bacteria, as well as good inhibitory properties (minimum inhibitory concentration).
Experimental investigation into machining performance of magnesium alloy AZ91D under dry, minimum quantity lubrication, and nano minimum quantity lubrication environments
Authors: Kumar Ajay , Gill Simranpreet Singh , Singh Gurpreet , Sharma Shubham , Dwivedi Shashi Prakash , Mohammed Kahtan A. , Sharma Kuldeep , Kozak Dražan , Hunjet Anica , Abbas Mohamed
Published: June 2024

Due to its low density, magnesium is recognized as a lighter metal and it is favorable for frequent use in industries. It is used in aerospace, biomedical, automotive, and other industrial applications. Magnesium is a promising element that is vital for reducing emissions, improving efficiency, protecting the environment, and enhancing the machine economy. This study analyzes the influence of various cutting environments and parameters on the turning operation of magnesium base alloy (AZ91D). Aluminum 9% and Zinc 1% is the main constituent of AZ91D. The machining process was accomplished using dry, minimum quantity lubrication (MQL), and nano minimum quantity lubrication (NMQL) environments based on their influence on surface roughness (SR) and temperature. Under certain circumstances, it was observed that SR decreases with the increase in the cutting velocity (V
c), feed rate, and depth of cut. During cutting of AZ91D in dry conditions, it is preferred to use a moderate speed. Higher temperature was recorded during dry conditions which can significantly reduce the life span of the tool. MQL and NMQL have reduced the cutting temperature by a margin of 25–40% compared to dry machining, thus improving tool life. NMQL has shown decent cooling results compared to other cooling systems.
Patterns of interaction between experiential and interpersonal meanings in student texts in Spanish: grounds for system-based applications in an academic writing context
Authors: Ignatieva Natalia , Rodríguez-Vergara Daniel , Zamudio-Jasso Victoria , Jiménez-Sánchez Guillermo
Published: June 2024

This paper presents a systemic functional analysis of process types in academic texts in Spanish from the Appraisal Theory perspective in order to establish connections and observe the interaction of the experiential and interpersonal metafunctions and the systems of Transitivity and Attitude. Our purpose is to explore how student writers use process types to construe their specific disciplinary experience, how they inscribe attitudinal meanings in their texts, and how the Transitivity and Attitude systems interact in student choices in academic writing. We used student texts from the CLAE corpus (Ignatieva, Natalia & M. Cecilia Colombi (eds.). 2014. CLAE: El lenguaje académico en México y los Estados Unidos, un análisis sistémico funcional. Mexico: UNAM). The texts belong to two disciplines (literature and history) and to three text types, or genres, labeled in the university setting as essays, reviews, and question-answers. The methodology included a quantitative and qualitative analysis of 1) process type realizations, 2) expressions of Attitude, classified according to type (Affect, Judgment, Appreciation) and 3) interaction between the process types and Attitude types. This was followed by a comparative analysis between the disciplines and genres, detecting both the similarities and the differences. In addition, some patterns that combine process types and Attitude expressions were found. Such patterns could represent an important tool in the teaching of academic writing in Spanish.
Presence of metabolic syndrome markers in very low birth weight ex-premature infants during early adolescence
Authors: Dinerstein Alejandro , Aspres Norma , Nieto Ricardo , Saure Carola , Cimbaro Canella Raul , Casale Maria Rosa , Moiron Maria del Carmen , Kasten Laura , Benitez Alicia , Galindo Ana , Conti Roxana
Published: June 2024

Objectives

To investigate the association of growth patterns with overweight/obesity and markers of metabolic syndrome in ex-premature adolescents; to assess the relationship between the increase (1 SD) in Z-score weight at term and at 2 years with outcomes in adolescents with or without intrauterine growth restriction; and to evaluate the association between the Cook criteria and overweight/obesity according to body mass index.

Methods

Cohort, retrospective, analytical study. Population: adolescents born weighting<1,500 g.

Results

One hundred twenty-seven adolescents (11.3 years) were included. There is an association between the 1 SD increase in the percentile (Pc) of weight at 40 weeks and at 2 years in the population with adequate birth weight (PCA) with insulin levels, resistance, and sensitivity at 11 years. Catch-up at 2 years was associated with significantly higher proportion of HDL value<41 (18.75 vs. 5.36 %) OR 4.08 95% CI (1.04–16.05) p=0.031. Overweight/obesity was associated with waist circumference index>0.5, HDL<41, and with blood pressure greater than Pc 90 for sex and height.

Conclusions

In preterm infants, a 1 SD increase in weight Z score at 40 weeks and 2 years was predictive of metabolic and cardiovascular disorders in adolescence.
Lycopene inhibits pyroptosis of endothelial progenitor cells induced by ox-LDL through the AMPK/mTOR/NLRP3 pathway
Authors: Tan Chujun , Chen Junqiu , Tu Tengcan , Chen Lifang , Zou Jun
Published: June 2024

The malfunction of endothelial progenitor cells (EPCs) due to ox-LDL is a risk contributor for arteriosclerotic disease. Meanwhile, lycopene possesses anti-inflammatory and antioxidative qualities. This investigation aimed to determine if lycopene can protect EPCs from ox-LDL-induced damage and to elucidate the underlying mechanism. The effects of lycopene on the survival, migration, and tube-forming capacity of EPCs were determined via in vitro assays. Expression of proteins related to pyroptosis and cellular proteins related to AMPK/mTOR/NLRP3 signaling was determined by western blot/flow cytometry. Our results demonstrated that lycopene treatment significantly enhanced proliferation, tube formation, and migration of EPCs stimulated by ox-LDL. Additionally, lycopene was found to suppress pyroptosis in ox-LDL-induced EPCs through the activation of AMPK, which led to the inhibition of mTOR phosphorylation and subsequent downregulation of the downstream NLRP3 inflammasome. In summary, our study suggests that lycopene mitigates ox-LDL-induced dysfunction in EPCs and inhibits pyroptosis via AMPK/mTOR/NLRP3 signaling. Our study suggests that lycopene may act as promising therapies for preventing atherosclerosis.
On oblique interaction of water waves with semi-infinite submerged elastic plate
Authors: Mirza Tooba , Hassan Mahmood-ul , Nawaz R.
Published: June 2024

This article relates with the interaction of water waves obliquely incident on a semi-infinite submerged elastic plate of insignificant thickness. The solution is obtained using method of mode matching. Reflection coefficients under the variation of various physical parameters: submergence depth, angle of incidence, stiffness of elastic plate and Poisson’s ratio, for two different edge conditions (i) pinned edge condition and (ii) sliding edge condition are discussed. Convergence and validation of results is also given. Behaviour of the velocity potentials around semi-infinite submerged elastic plate for different physical parameters are also analyzed. The consequences are explained through numerical and graphical results. More over energy balance relation is also discussed.
Violence/Visibility
Authors: Hansen James
Published: June 2024

In conjunction with her NEA Media Arts Residency at the Institute for Electronic Arts (IEA), artist Lorelei d’Andriole and I held the following conversation in a small, empty classroom on the fourth floor of Harder Hall on the campus of Alfred University (NYSCC). d’Andriole is an artist, educator, and writer whose work is at the intersection of intermedia and transgender studies. A day before the conversation she presented a lecture titled “Trans Embodiment and Intermedia Art,” which became the launching point for our conversation. “Oh, we’ll just sit and chat about your practice, your time at the IEA residency, and the idea of queer sound.” But we went on for nearly two and a half hours. As such, this interview has been edited and refined for publication.
OSTRACODS AND INTERREGIONAL CORRELATION BETWEEN THE ORDOVICIAN SECTIONS OF NORTHEAST RUSSIA (ERIEKHE RIVER BASIN) AND THE SIBERIAN PLATFORM
Authors: ГОНТА Т.В. , КАНЫГИН А.В. , СОБОЛЕВСКАЯ Р.Ф.
Published: June 2024

В разрезах среднего ордовика бассейна р. Эриехе, имеющих ключевое значение для сопоставления региональных стратиграфических подразделений Северо-Востока России с Общей стратиграфической шкалой (по граптолитам), выявлен богатый комплекс остракод, который существенно дополняет палеонтологическую характеристику среднего ордовика этого региона. Анализ стратиграфического распространения полученных комплексов позволил в значительной степени уточнить соотношение свит Эриехенской структурно-фациальной зоны (СФЗ) с региональными стратиграфическими подразделениями Северо-Востока России и по однотипным комплексам остракод с горизонтами Сибирской платформы. Совместные находки остракод и граптолитов в изученном разрезе позволяют сопоставить горизонты Северо-Востока России и Сибирской платформы с ярусами Общей стратиграфической шкалы.

In the Middle Ordovician sections of the Eriekhe River basin, which are of crucial importance to correlate the regional stratigraphic units of Northeast Russia and the General Stratigraphic Scale (by graptolites), we identified a diverse ostracod assemblage. The new data substantially supplement the Middle Ordovician paleontology of this region. The analysis of the stratigraphic distribution of these assemblages made it possible to verify the correlation of the Eriekhe Structural Facies Zone (SFZ) suites with regional stratigraphic units of the Northeast Russia and, based on homotypic ostracod assemblages, with the horizons of the Siberian Platform. The joint findings of ostracods and graptolites in the studied section allowed us to correlate the horizons of the Northeast Russia and the Siberian platform with the stages of the General Stratigraphic Scale.
An artificial neural network (ANN) modelling approach for evaluating turbidity properties of paper recycling wastewater
Authors: Kardeş Serkan , Özkan Uğur , Bayram Okan , Şahin Halil Turgut
Published: June 2024

A pre-treatment process was evaluated in this work for wastewater from paper recycling using microwave technology followed by rapid precipitation of contaminants through centrifugation. Artificial neural networks (ANNs) were used to analyze and optimize the turbidity values. Thirty experimental runs were utilized including microwave (MW) power, duration, centrifuge time, and centrifuge speed as input variables, generated by the Central Composite Full Design (CCFD) approach. The experimental turbidity ranged from 8.1 to 19.7 NTU, while predicted values ranged from 8.4 to 19.7 NTU by ANN. The ANN model showed a robust prediction performance with low mean squared error values during training and testing. Moreover, high R2 values showed a remarkable agreement between the experimental observations and ANN predictions. The results obtained from the input values (A:150.00, B:60.00, C:15.00, D:30.00) of sample 2, which gave the lowest turbidity value, showed the most removal of pollution. The results obtained from the input values (A:250.00, B:60.00, C:7.00, D:20.00) of sample 30, which gave the highest turbidity value, showed the least removal of pollution. The results showed that increasing RPM and time of the centrifugation process significantly affected the removal of pollution in wastewater.
Research on color and texture characteristics and visual perception of custom wardrobe finishes
Authors: Zhang Jiale , Chen Yushu
Published: June 2024

In recent years, the custom wardrobe market has been steadily developing. While meeting the functional needs of users, it is gradually shifting towards aesthetic preferences. Rapidly grasping users’ preferences for the appearance of custom wardrobes is a key focus of current research. This study collected a large number of decorative surface images of custom wardrobes and objectively analyzed the design features based on color moments and Tamura texture feature data in computer image analysis methods. K-means cluster analysis was performed on the feature data. Collected images of the points closest to the cluster centers were further screened to select representative finish images, and finally a questionnaire survey was conducted at Nanjing Forestry University, with the help of semantic differential method and factor analysis. The characteristics of the samples were comprehensively summarized to infer design elements. The study found that warm-toned, medium-low saturation, and medium brightness surfaces were preferred by the panel. Different colors, contrasts, saturations, brightness, element features, and arrangements have significantly different effects on visual perception. These conclusions can provide a reference for subsequent custom wardrobe design.
Common vices in pigeons
Authors: Ashraful Kabir M
Published: June 2024

Out of nine vices in pigeons’ behaviour and expression through vices or bad habits were scientifically described. Within these there were food related vices 2=22.22%, nervousness 1=11.11%, fight and sexual both 3=22.22%. Devoid of original pair mating its male partner tries to mate with another squab or female of different pair which considered great vices in pigeons for losing its purity. Fighting vices are related to sexual characteristics and that is why result suggests that in both cases the occurrence of the percentage is same. Food related vices are in second position and nervousness is psychological. All bad habits come in pigeons due to maladaptive or abnormal environment and some from their abnormal physiology. From the above vices pigeon keepers face food losses, inbreeding depression and purity, and their fighting causes injuries of chicks. Careful and keen handling of pigeons is the precursor for avoiding such vices.
Biodiversity and prevalence of chewing lice on local poultry
Authors: Abed El Aziz Maher Mohamed , Kalefa Mansur Mohamed , Mahmoud Najat Mohammed , Mustafa Allamoushi Sumaya
Published: June 2024

Biodiversity and prevalence of chewing lice on local poultry (Gallus Gallus Domesticus, family Phasianidae) have been successfully determined for five selected sites around Qaser Bin Ghashir region, Libya between November 2017 and April 2018. The total investigated chicken samples are 135, and the infected samples were found to be 94. Ticks, fleas, and mites were detected in a small quantity in addition to three different species of biting lice. The obtained results show that Asuani has the lowest amount of ectoparasites, while Suq AL-kamees samples were infected completely by 100 % prevalence of chewing lice. The calculated total average prevalence percentage (TAP, %) of all sites are ≤70%. Three species of lice were detected and identified on the local poultry, Menopon gallinae, Menacanthus stramineus, and Lipeurus caponis. The most abundant species in the study area is Menopon gallinae with prevalence percentage about ≈ 69.84%. A comparison between chewing lice around Qaser Bin Ghashir region in Libya and other countries has been carried out and it was found that (TAP, %) for Algeria > (TAP, %) for Libya > (TAP, %) for USA. Finally, the study proves that Libya contains a mild status of biodiversity.
Comparative life-style of pigeons in rural and urban areas of Bangladesh
Authors: Kabir M Ashraful
Published: June 2024

Urban areas are now more advanced on pigeons rearing than rural areas of Bangladesh. Urban people well known about pigeons and they get medical facilities from various corners especially vet. Moreover, they take it as a farm-based bird. In village, people rear it as a free range system. So, that pigeons are not their under control. Village pigeons go to the field and take insecticides and feed its young and ultimately their death is very common. Urban people take part in various seminars on pigeons, internet browsing and television program.
Divulgação científica: conceitos, aproximações, diferenças e relação com feiras de ciências
Authors: Rosa Soares Tamires , Maraschin André de Azambuja , Scaglioni Cícero Gularte , Araujo Rafaele Rodrigues de , Dorneles Pedro
Published: June 2024

Neste trabalho apresenta-se uma revisão da literatura, em que foram reunidos e discutidos um conjunto de trabalhos científicos visando um aprofundamento teórico a fim de contribuir para elucidação dos conceitos e área de abrangência da Divulgação Científica (DC) e sua articulação com as Feiras de Ciências (FCs). São abordados aspectos relacionados: a uma breve biográfica do cientista José Reis e suas contribuições para a DC no Brasil; as terminologias associadas a DC e suas origens; as questões que permeiam os propósitos e práticas em divulgação científica; as contribuições da DC para o Ensino e; a estreita relação da DC com as FCs. A partir da discussão feita, argumentamos que a Feira de Ciências constitui um importante meio para DC dialógica, na acepção de Paulo Freire, visto que se trata de um espaço de troca e socialização dos conhecimentos científicos. Além disso, torna-se necessário conduzir um maior número de estudos a fim de contribuir para um melhor tratamento da temática e investigar as suas potencialidades
Pathogens associated with seafood exports from Southeast Asia to the European Union: Analysis of the Rapid Alert System for Food and Feed (1997–2020)
Authors: Odeyemi O. A. , Somorin Y. M. , Ateba C. N. , Onyeaka H. , Anyogu A. , Amin M. , Dewi F. R. , Stratev D. , Oko J. O. , Kasan N. A.
Published: June 2024

Over the years, the need for healthy food has increased the demand for seafood in the international market. However, despite the high global demand, seafood is highly perishable and may harbour pathogenic microorganisms that could be transferred to humans during handling, processing, and consumption. Therefore, establishing strict food safety standards and risk communication tools is critical to prevent the infiltration of unsafe seafood into global markets. In the global food supply chain, the European Union (EU) is the largest importer of seafood, while more than 80% of global seafood production is from Asia. Additionally, Southeast Asian countries account for 32% of global seafood production. Therefore, this study aimed to evaluate the microbiological safety of seafood imported from Southeast Asia to the EU by analysing alert notifications in the Rapid Alert System for Food and Feed (RASFF) database. The RASFF is a tool used for reporting and communicating food safety-related risks among the EU Member States and other members of the RASFF network. A total of 301 notifications of pathogens in seafood originating from four Southeast Asian countries (Malaysia, Thailand, Vietnam, and Indonesia) were reported by 19 EU countries, including the United Kingdom (1997-2020). More than 56% of the pathogen notifications involved crustaceans and related products. Fifty-two percent of the notifications involved seafood from Vietnam, while 30% of the notifications from EU member countries were from Italy. Vibrio spp. was the most predominant bacterial pathogen responsible for 50% of the notifications, followed by Salmonella spp. and Clostridium accounting for 38% and 2% of the notifications, respectively. Norovirus was implicated in 10% of the notifications, and this was only in clams from Vietnam. These pathogens pose a risk to consumers’ health, indicating the need to enforce stringent food safety standards in producing countries to ensure the safety of seafood exported to other parts of the world. These actions will ultimately prevent economic loss associated with the rejection, destruction or import restrictions of contaminated seafood originating from Southeast Asia to the EU.
Review of stray dog management: dog days in the European countries
Authors: Papavasili Th. , A. Kontogeorgos A. Kontogeorgos , Mavrommati A. , Sossidou E. N. , Chatzitheodoridis F.
Published: June 2024

Western societies face a major challenge to control their stray dog population due to its uncontrolled propagation and abandonment. Despite the management programmes implemented in many European countries, the reduction of the stray dog population has not been achieved. This is not only related to dogs’ well-being, but also to their coexistence with humans, especially in urban areas. Problem-solving approaches vary in different countries, as there is no common legislation in the European Community dealing with stray dog control. To realise this research study, data from secondary sources, as well as data collected from thirteen European countries were analysed. A comparative overview of policies and measures was examined to show that stray dog population management depends on the policy implicated by each country. In contrast to countries of northern Europe, significant problems were faced mainly by the countries of southern Europe and the Balkans. The purpose of this paper was to present the legal framework of management for the stray dog population in accordance with what is implemented in different EU countries and to unveil the need for action for a common European Community Directive or Regulation dealing with stray dog control.
Project Spotlight: Precast concrete apartments match creative energy of Chicago
Authors: Atkinson Wiiliam
Published: June 2024

The Lucy Gonzalez Parsons Apartments is a seven-story, 100-unit affordable housing com¬plex in Chicago, Ill. One notable feature of the project is that it was a totally prefabricated build, involving the use of hollow-core floors and ceilings, exterior and interior structural wall panels, DELTABEAM columns, and precast concrete stairs provided by Wells Concrete of Albany, Minn.
Perbandingan Kelimpahan Zooplankton Berdasarkan Mesh Size di Sekitar Areal Pertambakan Kecamatan Marana Kabupaten Maros
Authors: Umar Nur Asia , Hatta Muh - , Rustam Agustin - , Hendrajat Erfan Andi , Aqmal Amal -
Published: June 2024

Kajian mengenai kelimpahan zooplankton secara parsial selama ini adalah menggunakan ukuran plankton net tertentu untuk jenis zooplankton tertentu, sehingga dibutuhkan kajian secara simultan yang membandingkan antara berbagai mesh zise dalam sebuah lokasi, untuk mengetahui mesh size yang paling tepat digunakan dalam pengambilan sampel zooplankton.  Tujuan penelitian ini adalah membandingkan jumlah jenis dan kelimpahan zooplankton yang tersaring antar mesh size plankton net yang berbeda di areal pertambakan. Penelitian dilakukan pada musim barat yaitu bulan Maret 2023 hingga Mei 2023 bertempat di kawasan Instalasi Tambak Percobaan Marana Balai Riset Perikanan Budidaya Air Payau dan Penyuluhan Perikanan (BRPBAPPP), Kecamatan Lau, Kabupaten Maros, Propinsi Sulawesi Selatan yang dibagi atas 6 stasiun. Pengukuran parameter lingkungan dilakukan secara in-situ dan ex-situ.  Plankton net yang digunakan sebagai perlakuan yaitu 25µm, 40µm 60µm.  Pengukuran kualitas air in-situ dan pengambilan sampel plankton dilakukan per dua minggu.  Analisis statistik yang digunakan adalah Analisis One Way Anova, Uji lanjut Post-Hoc Test  menggunakan metode Tukey untuk membandingkan rata-rata kelimpahan dan jumlah spesies antar mesh size. Hasil analisis menunjukkan bahwa terdapat perbedaan jumlah spesies yang tersaring berdasarkan mesh size plankton net yaitu plankton net size 60 µm berbeda jumlah spesies yang tersaring dengan mesh size 25µm dan 40µm, tetapi 25µm dan 40µm tidak berbeda. Rata rata kelimpahan zooplankton pada mesh size 25 µm (255 ind/l) berbeda nyata lebih tinggi dibandingkan dengan rata-rata kelimpahan zooplankton pada mesh size 60 µm (60 ind/l).  Rata-rata kelimpahan 40 µm (106 ind/l) tidak berbeda dengan 25 µm maupun 60 µm.
GROWTH AND REPRODUCTIVE PERFORMANCES OF RAMIREZI (Mikrogeophagus ramirezi) FED WITH DIFFERENT FEED TYPES
Authors: Azizah Azizah , Elisdiana Yeni , Adiputra Yudha Trinoegraha , Sarida Munti
Published: June 2024

The Ramirezi (Mikrogeophagus ramirezi) is an ornamental fish in high demand with significant economic value. The high demand for this commodity has yet to be met by the quantity of fry production generated by farmers. It is due to the Ramirezi's long maturation time and slow growth, which are influenced by factors such as the feed used. This study aimed to evaluate the effect of feeding with different feed types on the growth and development of the gonads of prospective Ramirezi broodstocks. The research design employed a completely randomized design (RAL) with four treatments and three replications, using a combined ratio of artificial feed to bloodworm (Chironomus sp.): (A) 3:0, (B) 0:3, (C) 2:1, and (D) 1:2, for 45 days of culture. The results showed that a combination of artificial feed and bloodworm in a 1:2 ratio increased the absolute body growth of Ramirezi by 1.267 g, with a positive allometric growth pattern. This ratio also affected the egg diameter, leading to a higher percentage of broodstock maturing more quickly and improving reproductive performance, as seen in the parameters of the number of eggs produced (ranging from 80-200 eggs), a 96.83% fertilization rate, and a 91.80% hatching rate. Therefore, researchers and farmers can combine artificial feed and bloodworms in a 1:2 ratio in Ramirezi cultivation activities.Ramirezi (Mikrogeophagus ramirezi) adalah salah satu ikan hias yang banyak diminati dengan nilai ekonomis tinggi. Tingginya permintaan terhadap komoditas ini belum diimbangi dengan jumlah produksi benih yang dihasilkan oleh pembudidaya. Hal tersebut karena secara biologis ramirezi memiliki waktu maturasi yang cukup lama dan pertumbuhan yang lambat. Salah satu factor, yang dipengaruhi oleh beberapa faktor seperti pakan yang digunakan. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian pakan dengan jenis pakan berbeda terhadap pertumbuhan dan perkembangan gonad calon induk ramirezi. Rancangan penelitian yang digunakan yaitu rancangan acak lengkap (RAL) dengan empat perlakuan dan tiga ulangan, menggunakan rasio kombinasi pakan buatan dengan cacing sutra (Chironomus sp.): (A) 3:0, (B) 0:3, (C) 2:1, dan (D) 1:2, selama 45 hari pemeliharaan. Hasil penelitian menunjukkan bahwa pemberian kombinasi pakan buatan dan cacing sutra dengan rasio 1:2 mampu meningkatkan pertumbuhan bobot mutlak ramirezi sebesar 1,267 g, dengan pola pertumbuhan alometrik positif. Rasio ini memberikan pengaruh terhadap diameter telur, menghasilkan persentase induk yang lebih cepat matang gonad serta meningkatkan performa reproduksi, terlihat dari parameter jumlah telur yang dihasilkan (berkisar 80-200 butir), derajat pembuahan 96,83%, dan derajat penetasan sebesar 91,80%. Oleh karena itu, peneliti dan pembudidaya dapat mengombinasikan pakan buatan dan cacing sutra dengan rasio 1:2 pada kegiatan budidaya ramirezi.
PENGARUH WARNA LATAR WADAH TERHADAP PERTUMBUHAN DAN SINTASAN LARVA IKAN NILEM (Osteochilus hasselti)
Authors: Heltonika Benny , Hasyim Dio Izmi , Sukendi Sukendi
Published: June 2024

Penglihatan bagi sebagian besar larva ikan memiliki peran yang sangat penting yang digunakan dalam mendeteksi keberadaan pakan. Salah satu faktor yang memengaruhi penglihatan dalam kondisi alami bergantung pada warna latar habitat yang disukai ikan tersebut. Tujuan dari penelitian ini yaitu mengetahui pengaruh warna latar wadah terhadap pertumbuhan dan sintasan larva ikan nilem (Osteochilus hasselti). Penelitian ini dilakukan dengan metode eksperimen menggunakan rancangan acak lengkap satu faktor dengan lima perlakuan warna latar media dengan tiga ulangan. Perlakuan dalam penelitian ini adalah perbedaan warna wadah yaitu Wo (wadah oranye), Wbr (wadah biru), Wm (wadah merah), Wb (wadah bening), dan Wh (wadah hitam). Penelitian ini menggunakan larva ikan nilem berusia 5 hari setelah menetas dengan ukuran 0,5 ± 0,03 cm dengan pada tebar 2 ekor L-1. Selama pemeliharaan, ikan diberi pakan Artemia selama 10 hari pertama dan dilanjutkan dengan Moina sp. Hasil penelitian menunjukkan bahwa warna latar wadah berpengaruh nyata terhadap pertumbuhan dan kelangsungan hidup larva ikan nilem (P<0,05). Perlakuan terbaik adalah Wo (wadah oranye) dengan pertumbuhan bobot mutlak 1,08 ± 0,03 g, pertumbuhan panjang mutlak 3,04 ± 0,02 cm, laju pertumbuhan spesifik 14,57 ± 0,07 % hari-1, dan sintasan 93,33 ± 2,89 %. Hal ini ditunjukkan dengan respons larva ikan nilem terhadap pakan yang diberikan.  Vision ability has a very important role for most fish larvae which are used to detect the presence of food. One of the factors that affecting vision in natural conditions depends on the favorable background color of the fish’s habitat. The aim of study was to determine the effects of the background colors of the container on the growth and survival of bonylip barb (Osteochilus hasselti) larvae. The experimental method has used in this study was one factor completely randomized design with five different background’s colors of the container with three replications for each treatment. The treatments in this study were different color containers, namely Wo (orange container), Wbr (blue container), Wm (red container), Wb (clear container), and Wh (black container). This study used bonylip larvae aged 5 days after hatching with a size of 0,5±0,03 cm with a stocking of 2 individuals L-1. During the rearing period, the fish were fed Artemia for the first 10 days and continued with Moina sp. The results showed that the background’s color of the container significantly affected on the growth and survival of bonylip barb larvae (P<0.05). The best treatment was Wo (orange container) with an absolute weight growth of 1.08 ± 0.03 g, an absolute length growth of 3.04 ± 0.02 cm, a specific growth rate of 14.57 ± 0.07% day-1, and a survival of 93.33 ± 2.89%. It was indicated with the response of bonylip barb fish larvae to the administered feed.
PENGARUH PENGGUNAAN Artemia salina YANG DIPERKAYA DENGAN ASAM AMINO TERHADAP SINTASAN LARVA RAJUNGAN (Portunus pelagicus Linn. 1758) STADIA ZOEA
Authors: Dananjaya Wayan Kantun , Sukriani Sukriani , Latief Nursidi , Cahyono Indra
Published: June 2024

Sintasan dalam usaha budidaya rajungan sangat ditentukan oleh pakan yang diberikan selama pemeliharaan. Penelitian ini bertujuan untuk mengevaluasi penambahan asam amino pada Artemia salina pada dosis yang berbeda untuk meningkatkan sintasan larva rajungan. Penelitian dirancang menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan tiga ulangan. Perlakuan diberikan dengan cara memperkaya A. salina menggunakan multi asam amino sesuai dengan dosis meliputi perlakuan A: 0,0; B: 2,5; C: 5,0; dan D: 7,5 ppm. Hasil penelitian memperlihatkan bahwa sintasan larva rajungan pada masing-masing perlakuan yaitu A.11,85 ± 0,40%; B. 16,18 ± 0,18%; C. 37,68 ± 0,20%; dan D. 50,35 ± 0,10% dengan pola hubungan yang polinomial antarpemberian dosis multi asam amino dengan sintasan larva rajungan. Berdasarkan hasil penelitian ini, disimpulkan bahwa penambahan multi asam amino pada A. salina sebagai pakan alami mampu meningkatkan sintasan larva rajungan pada stadia zoea. Survival in blue swimming crab cultivation is largely determined by the feed provided during the rearing period. This study aimed to evaluate the addition of amino acids to Artemia salina at different doses to increase the survival of blue swimming crab larvae. The study was designed using a completely randomized design (CRD) with four treatments and three replications. Treatments were applied by enriching A. salina using multi amino acids according to the doses including treatment A: 0.0; B: 2.5; C: 5.0; and D: 7.5 ppm. The results of the study showed that the survival of blue swimming crab larvae in each treatment was A. 11.85 ± 0.40%; B. 16.18 ± 0.18%; C. 37.68 ± 0.20%; and D. 50.35 ± 0.10 with a polynomial relationship pattern between administration of multi-amino acids doses and survival of blue swimming crab larvae. Based on the results of this study, it was concluded that the addition of multi amino acids to A. salina as a live feed was able to increase the survival of blue swimming crab larvae at the zoea stage.
PENGARUH PENAMBAHAN BINDER TEPUNG RUMPUT LAUT (Eucheuma spinosum) DENGAN PERSENTASE BERBEDA TERHADAP KANDUNGAN NUTRISI PAKAN UDANG
Authors: Salma Alfiatus , Arisandi Apri
Published: June 2024

Binder carboxy methyl cellulose (CMC) biasa digunakan dalam formulasi pakan udang, tetapi mempunyai harga mahal dan tidak memberi dampak terhadap penambahan nutrisi pakan. Penelitian ini bertujuan untuk menemukan bahan binder pengganti CMC yang lebih murah dan memberi dampak penambahan nutrisi dalam pakan udang. Dosis perlakuan binder tepung rumput laut dalam pakan uji sebesar 5% (P1), 10% (P2), 15% (P3), dan 20% (P4). Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan dan tiga ulangan, antara lain (P0) binder CMC sebagai kontrol dan (P1-P4) binder tepung rumput laut. Pakan perlakuan diuji kadar air, protein, dan lemaknya dengan metode analisis proksimat. Hasil nilai kadar air pada P0 (3,3%), P1 (3,8%), P2 (4,2%), P3(6%), dan P4 (6,3%), kadar protein pada P0 (40,40%), P1 (28,43%), P2 (40,40%), P3 (32,90%), dan P4 (28,40%), kadar lemak pada P0 (9,97%), P1 (9,98%) dan P1-P4 (9,97%). Penelitian ini menunjukkan bahwa perlakuan penambahan binder CMC dibandingkan dengan perlakuan penambahan binder tepung rumput laut tidak memberikan pengaruh yang nyata terhadap penambahan nutrisi pakan udang. Hasil tersebut membuktikan bahwa tepung rumput laut dapat menggantikan CMC sebagai binder dalam formulasi pakan udang.The binder, carboxy methyl cellulose (CMC), is commonly used in shrimp feed formulations, but it is expensive and has no impact on adding feed nutrition. This experiment aimed to find a binder substitute for CMC that is cheaper and has the effect of adding nutrients to shrimp feed. The doses of binder from seaweed meal treatment in the test feed were 5% (P1), 10% (P2), 15% (P3), and 20% (P4). This study used a completely randomized design with five treatments and three replications, including (P0) CMC binder as a control and (P1-P4) seaweed meal binder. The test feed were tested for water, protein and fat content using the proximate analysis method. Results of water content in P0 (3.3%), P1 (3.8%), P2 (4.2%), P3 (6%), and P4 (6.3%), protein content in P0 (40 .40%), P1 (28.43%), P2 (40.40%), P3 (32.90%), and P4 (28.40%), lipid content in P0 (9.97%), P1 (9.98%) and P1-P4 (9.97%). This experiment showed that the treatment with the addition of CMC binder compared to the treatment with the addition of seaweed meal binder did not have a significant effect on the addition of nutrients to shrimp feed. These results proved that seaweed meal can replace CMC as a binder in shrimp feed formulations.
IMMUNOSTIMULATORY EFFECTS OF ULVAN ON TRYPSIN-MEDIATED PROTEIN DIGESTION IN THE GUT OF PACIFIC WHITELEG SHRIMP (Litopenaeus vannamei)
Authors: Azhar Nuril , Yudiati Ervia , Ambariyanto Ambariyanto , Alghazeer Rabia , Trianto Agus
Published: June 2024

Litopenaeus vannamei has emerged in the aquaculture industry. Production consistency, nutrition, and disease management play critical roles, particularly the digestive enzymes such as trypsin. This study assesses Ulvan, an immunostimulant from Ulva lactuca, on shrimp trypsin activity. Trypsin has been found to significantly enhance the activity of hemocyanin phenoloxidase, a crucial component of humoral immunity. This study aims to evaluate the potency of ulvan related to trypsin as an immunostimulant agent, extracted from Ulva sp.  Ulvan, extracted using various methods (P-HWE, O-HWE, P-A-HWE, and O-A-HWE), was evaluated using different doses of (0 g kg-1 (Control), 0.75 g kg-1 (ULV-0.75), 1.50 g kg-1 (ULV-1.50), and 3.00 g kg-1 (ULV-3.00) of feed). The O-A-HWE exhibited the fastest and highest increase in trypsin activity on day 4, surpassing the control on days 2, 3, 7, and 8. The P-HWE, O-HWE, and P-A-HWE also showed significant changes in trypsin activity compared to the control on specific days. Meanwhile, trypsin activity in Ulvan-fed shrimp did not significantly differ from the control on days 0 and 1. The differences emerged on day 2 and 3, notably between ULV-1.50 g kg−1 and ULV-0.75 g kg−1. The ULV-3.00 g kg−1 showed no significant difference from ULV-1.50 g kg−1. O-A-HWE demonstrated significant differences in trypsin activity compared to other Ulvan extracts, suggesting its potential to enhance shrimp health.Litopenaeus vannamei memiliki peran yang besar dalam industri akuakultur. Konsistensi dalam produksi, nutrisi, dan pengelolaan terhadap penyakit merupakan bagian yang sangat penting, terutama enzim pencernaan, di antaranya tripsin. Tripsin berfungsi untuk meningkatkan fenoloksidase hemosianin, yang peranannya penting untuk kekebalan. Studi ini mengevaluasi Ulvan, yaitu bahan yang bersifat imunostimulan dari Ulva sp., terhadap aktivitas tripsin udang. Ulvan, diekstraksi menggunakan berbagai metode (P-HWE, O-HWE, P-A-HWE, dan O-A-HWE), dievaluasi menggunakan perlakuan dosis yang berbeda (0 g kg-1 (Kontrol), 0,75 g kg-1 (ULV-0,75), 1,50 g kg-1 (ULV-1,50), dan 3,00 g kg-1 (ULV-3,00) pada pemeliharaan udang vaname selama 10 hari. O-A-HWE menunjukkan peningkatan aktivitas tripsin tercepat dan tertinggi pada hari ke-4, melebihi kontrol pada hari ke-2, 3, 7, dan 8. P-HWE, O-HWE, dan P-A-HWE juga menunjukkan perubahan signifikan dalam aktivitas tripsin dibandingkan dengan kontrol pada hari-hari tertentu. Sementara aktivitas tripsin dalam udang yang diberi Ulvan tidak berbeda secara signifikan dari kontrol pada hari ke-0 dan 1, perbedaan mulai terlihat pada hari ke-2 dan 3, terutama antara ULV-1,50 g kg−1 dan ULV-0,75 g kg−1. ULV-3,00 g kg−1 tidak menunjukkan perbedaan yang signifikan dari ULV-1,50 g kg−1. O-A-HWE menunjukkan perbedaan signifikan dalam aktivitas tripsin dibandingkan dengan ekstrak Ulvan lainnya, sehingga berpotensi dalam meningkatkan kesehatan udang.
Ovarian tissue cryopreservation by vitrification: The first Vietnam experience
Authors: Lan Nguyen TT , Tri Nguyen C , Diem Nguyen TN , Thu Tran B , Kiet Truong D , Vinh Dang Q , Tien Nguyen V , Tuong Ho M
Published: June 2024

Ovarian Tissue Cryopreservation (OTC) is a promising technique for reproductive preservation in women with cancer. Although this technique has been successfully applied in many Invitro Fertilization (IVF) centers worldwide, OTC has not been recently developed in Vietnam, and there are no publications of OTC. The study of OTC by vitrification at IVFMD, My Duc Hospital, was conducted from 2018 to 2023 with trial registration number NCT04666376. Ovarian tissues from each patient were divided into 3 groups: group 1, which consisted on fresh tissues as a control group; group 2, which consisted of vitrified tissues using the Ova Kit Type M (Kitazato, Japan); and group 3, which consisted of vitrified tissues using IVFMD medium. The findings showed that the rates of viable follicles and follicles with both healthy oocytes and CGs in groups 2 and 3 were significantly lower than in group 1, at [80.3% in group 1, 70% in group 2, and 65% in group 3] and [71.3% in group 1, 59.4% in group 2, and 58.3% in group 3] respectively (P < 0.05). The results aligned with previous findings due to the negative effects of cryopreservation. Moreover, it was illustrated that there was no change in the quality of tissue between the commercial medium and IVFMD’s medium in OTC.
Construction and evaluation of the diagnostic value of the recombinant anti-CD3ε antibody in the qualification of T cells by flow cytometry
Authors: Linh Nguyen Kim Khanh , Anh Truong Diep Phuong , Thuy Vo Le Thanh , Phat Vo Minh , Nguyen Lam Vy , Quan Nguyen Dang , Thao Nguyen Thi Thanh
Published: June 2024

Quantification of absolute and percentage values for T lymphocyte subsets is a common test for early detection, diagnosis, and treatment of immune-mediated diseases. By using monoclonal antibodies against CD3, CD4, and CD8 conjugated with fluorescent reagents, flow cytometry is a powerful technique to qualify CD3, CD4, and CD8─ positive T cells in the evaluation of primary (congenital) and secondary (acquired) immunodeficiencies such as HIV/AIDS, autoimmune diseases, leukemia, non-Hodgkin lymphoma, Hodgkin lymphoma, … However, almost all monoclonal antibodies used in hospitals around Vietnam are imported from foreign countries, increasing the financial burden on patients. Our study aims to create and evaluate the efficiency of a recombinant anti-CD3ε monoclonal antibody (mAb) obtained from the CHO-DG44 host cell system to identify antigen-specific T cells using flow cytometry. The monoclonal antibodies were purified from culture supernatant and have dimeric structures, a feature that is responsible for mAb activities. The flow cytometry results indicated that the recombinant anti-CD3εmAb was able to accurately qualify the cell-surface antigen in vitro. Furthermore, the mAb developed in this study specifically recognized T cells as opposed to other major human blood cell types such as B cells, NK cells, and monocytes in human peripheral blood. These data collectively suggested that anti-CD3εmAb expressed by CHO- DG44 cells has the potential for further development towards the application of CD3+ T cell quantification in disease diagnosis by using flow cytometry.
Transgenics with gonadal microinjection in dioecious Caenorhabditis nematodes
Authors: Son Le Tho , Thu Nguyen Thi
Published: June 2024

Biological research with nematode models is increasing to understand molecular phenomena related to genome, genetics, and biological comparison. Various methods have been developed to produce genetically modified organisms with desired characteristics. First successfully employed in 1985, the gonadal microinjection in Caenorhabditis elegans has proven highly effective for transformations. For other nematodes, this transformation methodology could play an important role in studying genetics and genomics within and among the species. Research methods with several other non-C. elegans round nematodes were adopted the microinjections to do transformation. In this study on transformation, we report the results of transgenics using different plasmids in eight dioecious species (C. portoensis, C. sp. 33, C. brenneri, C. nigoni, C. sinica, C. imperialis, C. nouraguensis, and C. remanei). We successfully obtained stable transgenes in the first four species but encountered failures in the last four.
“Alimentar de pessoal capacitado o desenvolvimento industrial do país”
Authors: Lopes Amorim Mario
Published: June 2024

Este artigo objetiva analisar a construção de representações sobre o ensino industrial como condição para o desenvolvimento do Brasil, na revista Ensino Industrial, entre 1964 e 1969. A publicação em tela divulgava as atividades da Divisão do Ensino Industrial (DEI) do MEC, em consonância com os padrões racionalizadores de conformação da força de trabalho, cuja base era a Teoria do Capital Humano, com destaque para a importância da cooperação dos EUA na área. A análise privilegia a contextualização histórica do período de circulação de revista, ao relacionar o treinamento da força de trabalho com os anelos da Doutrina de Segurança Nacional. Conclui-se que os textos de Ensino Industrial dão continuidade à orientação presente no período histórico anterior à ditadura empresarial-militar.
Dyplomacja trzęsień ziemi a zmiana pozycji międzynarodowej Syrii
Authors:   Uniwersytet Łódzki, Małecki Jacek
Published: June 2024

Syria is a state which has been affected by multiple series of disasters in the XXI century. Some of them has been natural caused, whereas there were some caused by human activity. Only for the first two decades of the current century, Syria has been the arena of the most complex military conflict, which significantly damaged the whole state and caused its partial international isolation. On the other hand, Syria has struggle with various types of natural disasters, such as drought from 2006 to 2010, numerous floods, the COVID-19 and Cholera outbreak and finally, the recent earthquake, occured in February 2023. The last phenomenon has lead numerous countries to express solidarity with Syria as well as to deliver the humanitarian aid to this country. Some of them are considered to be hostile towards Damascus and president Bashar el-Assad. In parallel to the humanitarian assistance, there was a diplomatic process, which led to full rehabilitation of Syrian president in the region and to full normalization diplomatic relations with other Arabic states as well as to start a dialogue with Turkey. In some analytical and media centers, the recent events have been described as an outcome of the earthquake diplomacy or disaster diplomacy, characterized as the international situation in which the natural disaster and the readiness to counterreaction against its effects, may create the opportunities to deescalate relations between the warrying states. Therefore, would it be accurate to consider the recent disaster in Syria able to create the new process as well as to transform the long-term strategies of a number of countries? This article will deal with analysing political process occured between Syria and the regional powers after the earthquake as well as it will try to answer the question how the international position of Syria has changed and crucially: does the recent earthquake have the impact on these issues?