bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-Viral Delivery of RNA Gene Therapy to the Central Nervous System
Authors: Hauck Ellen S. , Hecker James G.
Published: January 2022

Appropriate gene delivery systems are essential for successful gene therapy in clinical medicine. Lipid-mediated nucleic acid delivery is an alternative to viral vector-mediated gene delivery and has the following advantages. Lipid-mediated delivery of DNA or mRNA is usually more rapid than viral-mediated delivery, offers a larger payload, and has a nearly zero risk of incorporation. Lipid-mediated delivery of DNA or RNA is therefore preferable to viral DNA delivery in those clinical applications that do not require long-term expression for chronic conditions. Delivery of RNA may be preferable to non-viral DNA delivery in some clinical applications, since transit across the nuclear membrane is not necessary, and onset of expression with RNA is therefore even faster than with DNA, although both are faster than most viral vectors. Delivery of RNA to target organ(s) has previously been challenging due to RNA’s rapid degradation in biological systems, but cationic lipids complexed with RNA, as well as lipid nanoparticles (LNPs), have allowed for delivery and expression of the complexed RNA both in vitro and in vivo. This review will focus on the non-viral lipid-mediated delivery of RNAs, including mRNA, siRNA, shRNA, and microRNA, to the central nervous system (CNS), an organ with at least two unique challenges. The CNS contains a large number of slowly dividing or non-dividing cell types and is protected by the blood brain barrier (BBB). In non-dividing cells, RNA-lipid complexes demonstrated increased transfection efficiency relative to DNA transfection. The efficiency, timing of the onset, and duration of expression after transfection may determine which nucleic acid is best for which proposed therapy. Expression can be seen as soon as 1 h after RNA delivery, but duration of expression has been limited to 5–7 h. In contrast, transfection with a DNA lipoplex demonstrates protein expression within 5 h and lasts as long as several weeks after transfection.
Herbal Medicines—Are They Effective and Safe during Pregnancy?
Authors: Sarecka-Hujar Beata , Szulc-Musioł Beata
Published: January 2022

Since the teratogenicity of Thalidomide has been proven, herbal products are more commonly used in pregnancy to not only relieve morning sickness but also to fight infections. These products are frequently considered as natural and therefore harmless. However, herbs contain a number of active substances that, when used during pregnancy, can affect the development of the fetus. Often, pregnant women do not consult the usage of herbal medicines with a physician. The access to these products is easy and treatment of certain ailments with the use of herbs is common in many countries. The aim of the present literature review was to discuss available data regarding the efficacy and safety of cranberry, chamomile, Echinacea purpurea, garlic, ginger, Ginkgo biloba, and peppermint, which are used to counteract the most common ailments during pregnancy, i.e., infections and pregnancy-related ailments (e.g., nausea and vomiting, dizziness, and headache). Analysis of available data showed that ginger is one of the most extensively analyzed herbal remedies. The dose of ginger below 1000 mg per day may help to relief hypereremesis gravidarum, and such an amount of ginger did not increase frequency of adverse effects for either woman or developing fetus. Data regarding other herbs are most often heterogeneous and give conflicting results with no clear conclusions. However, all herbal products should be used with a special caution in pregnancy. Further high-quality human studies should be determined to confirm the safe doses of herbal products which could be used by pregnant or breast-feeding women.
The Effect of VPA Treatment on Radiolabeled DOTATATE Uptake: Differences Observed In Vitro and In Vivo
Authors: Klomp Maria , Hofland Leo , van den Brink Lilian , van Koetsveld Peter , Dogan Fadime , de Ridder Corrina , Stuurman Debra , Clahsen-van Groningen Marian , de Jong Marion , Dalm Simone
Published: January 2022

Background: To improve peptide receptor radionuclide therapy (PRRT), we aimed to enhance the expression of somatostatin type-2 receptors (SSTR2) in vitro and in vivo, using valproic acid (VPA). Methods: Human NCI-H69 small-cell lung carcinoma cells were treated with VPA, followed by [111In]In-DOTATATE uptake studies, RT-qPCR and immunohistochemistry analysis. Furthermore, NCI-H69 xenografted mice were treated with VPA or vehicle, followed by [177Lu]Lu-DOTATATE injection. Biodistribution studies were performed, and tissues were collected for further analysis. Results: VPA significantly increased SSTR2 expression in vitro. In animals, a statistically significant increased [177Lu]Lu-DOTATATE tumoral uptake was observed when VPA was administered eight hours before [177Lu]Lu-DOTATATE administration, but increased tumor SSTR2 expression levels were lacking. The animals also presented significantly higher [177Lu]Lu-DOTATATE blood levels, as well as an elevated renal tubular damage score. This suggests that the enhanced tumor uptake was presumably a consequence of the increased radiotracer circulation and the induced kidney damage. Conclusions: VPA increases SSTR2 expression in vitro. In vivo, the observed increase in tumoral [177Lu]Lu-DOTATATE uptake is not caused by SSTR2 upregulation, but rather by other mechanisms, e.g., an increased [177Lu]Lu-DOTATATE circulation time and renal toxicity. However, since both drugs are safely used in humans, the potential of VPA to improve PRRT remains open for investigation.
Insights from a Box–Behnken Optimization Study of Microemulsions with Salicylic Acid for Acne Therapy
Authors: Anicescu Maria-Cristina , Dinu-Pîrvu Cristina-Elena , Talianu Marina-Theodora , Ghica Mihaela Violeta , Anuța Valentina , Prisada Răzvan-Mihai , Nicoară Anca Cecilia , Popa Lăcrămioara
Published: January 2022

The present study brings to attention a method to develop salicylic acid-based oil in water (O/W) microemulsions using a tensioactive system based on Tween 80, lecithin, and propylene glycol (PG), enriched with a vegetable oat oil phase and hyaluronic acid. The systems were physically characterized and the Quality by design approach was applied to optimize the attributes of microemulsions using Box–Behnken modeling, combined with response surface methodology. For this purpose, a 33 fractional factorial design was selected. The effect of independent variables namely X1: Tween 80/PG (%), X2: Lecithin (%), X3: Oil phase (%) was analyzed considering their impact upon the internal structure and evaluated parameters chosen as dependent factors: viscosity, mean droplet size, and work of adhesion. A high viscosity, a low droplet size, an adequate wettability—with a reduced mechanical work—and clarity were considered as desirable for the optimal systems. It was found that the optimal microemulsion which complied with the established conditions was based on: Tween 80/PG 40%, lecithin 0.3%, oat oil 2%, salicylic acid 0.5%, hyaluronic acid 1%, and water 56.2%. The response surface methodology was considered an appropriate tool to explain the impact of formulation factors on the physical properties of microemulsions, offering a complex pattern in the assessment of stability and quality attributes for the optimized formulation.
An Oligomeric Sulfated Hyaluronan and Silk-Elastinlike Polymer Combination Protects against Murine Radiation Induced Proctitis
Authors: Steinhauff Douglas , Jensen Mark Martin , Griswold Ethan , Jedrzkiewicz Jolanta , Cappello Joseph , Oottamasathien Siam , Ghandehari Hamidreza
Published: January 2022

Semisynthetic glycosaminoglycan ethers (SAGEs) are short, sulfated hyaluronans which combine the natural properties of hyaluronan with chemical sulfation. In a murine model, SAGEs provide protection against radiation induced proctitis (RIP), a side effect of lower abdominal radiotherapy for cancer. The anti-inflammatory effects of SAGE have been studied in inflammatory diseases at mucosal barrier sites; however, few mechanisms have been uncovered necessitating high throughput methods. SAGEs were combined with silk-elastinlike polymers (SELPs) to enhance rectal accumulation in mice. After high radiation exposure to the lower abdominal area, mice were followed for 3 days or until they met humane endpoints, before evaluation of behavioral pain responses and histological assessment of rectal inflammation. RNA sequencing was conducted on tissues from the 3-day cohort to determine molecular mechanisms of SAGE–SELP. After 3 days, mice receiving the SAGE–SELP combination yielded significantly lowered pain responses and amelioration of radiation-induced rectal inflammation. Mice receiving the drug–polymer combination survived 60% longer than other irradiated mice, with a fraction exhibiting long term survival. Sequencing reveals varied regulation of toll like receptors, antioxidant activities, T-cell signaling, and pathways associated with pain. This investigation elucidates several molecular mechanisms of SAGEs and exhibits promising measures for prevention of RIP.
Demonstration of a Stereospecific Photochemical Meta Effect
Authors: Pham Hoai , Hunsley Madelyn , Yang Chou-Hsun , Wang Haobin , Reed Scott M.
Published: January 2022

A fundamental goal of photochemistry is to understand how structural features of a chromophore can make specific bonds within a molecule prone to cleavage by light, or photolabile. The meta effect is an example of a regiochemical explanation for photolability, in which electron donating groups on an aromatic ring cause photolability selectively at the meta position. Here, we show, using a chromophore containing one ring with a meta-methoxy group and one ring with a para-methoxy group, that two stereoisomers of the same compounds can react with light differently, based simply on the three-dimensional positioning of a meta anisyl ring. The result is that the stereoisomers of the compound with the same configuration at both stereogenic centers are photolabile while the stereoisomers with opposite configuration do not react with light. Furthermore, time-dependent density functional theory (TD-DFT) calculations show distinct excitation pathways for each stereoisomer.
Assessment of the Transfer of Trace Metals to Spontaneous Plants on Abandoned Pyrrhotite Mine: Potential Application for Phytostabilization of Phosphate Wastes
Authors: El Berkaoui Meryem , El Adnani Mariam , Hakkou Rachid , Ouhammou Ahmed , Bendaou Najib , Smouni Abdelaziz
Published: January 2022

The abandoned Kettara pyrrhotite mine (Marrakech region, Morocco) is a real source of acid mine drainage (AMD) and heavy metal pollution from previous mining operations—which has spread, particularly because of wind erosion. A store-and-release cover system made of phosphate wastes was built on the site for preventing AMD. To ensure the integrity of this cover and its durability, it is desirable to revegetate it (phytostabilization) with plants adapted to the edaphoclimatic conditions of the region. In this paper, a study was carried out on the spontaneous vegetation around the phosphate cover in order to consider the selection of plants to promote the stabilization of the Kettara mine tailings pond. Nine species of native plants with their rhizospheric soils growing in agricultural soils and tailings from the Kettara mine were collected, and metals (As, Cd, Co, Cu, Pb, Zn, Ni, Cr) were analyzed. The soil analysis showed that the tailings contained high concentrations of Cu (177.64 mg/kg) and Pb (116.80 mg/kg) and that the agricultural soil contained high concentrations of As (25.07 mg/kg) and Cu (251.96 mg/kg) exceeding the toxicity level (Cu > 100 mg/kg, Pb > 100 mg/kg, As > 20 mg/kg). The plant analysis showed low trace metal accumulation in Scolymus hispanicus, Festuca ovina, Cleome brachycarpa, Carlina involucrata and Peganum harmala. These species had a bioconcentration factor (BCF) greater than 1 and a translocation factor (TF) less than 1, demonstrating a high tolerance to trace metals. Therefore, they are good candidates for use in the phytoremediation of the Kettara mine tailings. These species could also potentially be used for the phytostabilization of the phosphate waste cover of the Kettara mine, thus completing the rehabilitation process of this area.
Morphological and Genome-Wide Evidence of Homoploid Hybridisation in Urospermum (Asteraceae)
Authors: Pellicer Jaume , Balant Manica , Fernández Pol , Rodríguez González Roi , Hidalgo Oriane
Published: January 2022

The genus Urospermum is distributed in the Mediterranean region and Macaronesia, and has been introduced to other extra-Mediterranean regions. Although the two species constituting the genus, U. dalechampii and U. picroides, are frequently found together, hybrids have so far only been reported once, from Morocco. However, we found certain individuals in Catalonia, whose intermediate morphology suggested a potential hybrid origin. In this study, we applied morphological and molecular methods to investigate the origin of those individuals. Intermediate features at phenotype, karyological, cytogenetic, and genomic levels were identified in morphologically intermediate individuals, supporting their homoploid hybrid origin. Chloroplast sequence data suggest that U. dalechampii is the maternal progenitor of the hybrid. Together with the intermediate traits displayed, the lack of fertile seeds suggests that hybrids are probably F1. Future monitoring studies will be, nonetheless, needed to evaluate the extent of hybridisation and its potential impact on the biology of the genus.
Impact of Safe Rock® Minerals, Mineral Fertilizers, and Manure on the Quantity and Quality of the Wheat Yield in the Rice–Wheat Cropping System
Authors: Ranva Santosh , Singh Yudh Vir , Jain Neelam , Bana Ram Swaroop , Bana Ramesh Chand , Aseri Gajender K. , Madar Raghavendra , Shokralla Shadi , Mahmoud Eman A. , El-Sabrout Ahmed M. , Elansary Hosam O.
Published: January 2022

Rice–wheat (RW) rotation is the largest agriculture production system in South Asia with a multifaceted role in maintaining the livelihood of people. The customary practices and indiscriminate use of synthetic fertilizers have culminated in the decline of its productivity and profitability during the past two decades, thus affecting the sustainability of wheat. Safe Rock® Minerals (SRM) is a multi-nutrient rich natural rock mineral with great potential to manage soil degradation, reducing the input of fertilizers, improving soil fertility, and plant health. Thus, a field trial was conducted at the research farm of ICAR—Indian Agricultural Research Institute, New Delhi from 2016 to 2018 to evaluate the impact of Safe Rock® Minerals (SRM) on biometric parameters, productivity, quality, and nutrient uptake by conventional wheat and System of Wheat Intensification (SWI) in the wheat–rice cropping system. The results indicate that SWI performed better in terms of growth, yield, and quality parameters than conventional wheat. Among nutrient management practices; the highest growth, yield, and yield attributes of wheat were achieved with the use of SRM application 250 kg ha−1 + 100% Recommended Dose of Fertilizer (RDF). SRM application also increased grain protein content significantly. In conclusion, the integrated use of SRM with organic manures can serve as an eco-friendly approach for sustainable wheat production.
Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange
Authors: Phanomchai Saowaros , Bodhipadma Kitti , Noichinda Sompoch , Leung David W. M.
Published: January 2022

Microshoots have been widely used for micropropagation. It may be necessary to store microshoots for a short period of time, for example in germplasm exchange needing transport to other research groups. Here, we investigated the short-term storability of alginate-encapsulated Persian violet (Exacum affine Balf. f. ex Regel) microshoots at 4 °C and 25 °C. After storage, the encapsulated microshoots were sown on basal Murashige and Skoog medium for germination and viability determination using tetrazolium chloride staining. The results showed that one or five microshoots encapsulated with a single alginate layer could be stored at 4 °C for up to 30 days, while the percentages of germination and viability of the microshoots encapsulated with two layers of alginate were greatly reduced upon storage. This is the first report on the storability of alginate-encapsulated multiple microshoots, which could be a more efficient way to encapsulate microshoots used for short-term cold storage.
The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected?
Authors: Valmonte-Cortes Gardette R. , Lilly Sonia T. , Pearson Michael N. , Higgins Colleen M. , MacDiarmid Robin M.
Published: January 2022

To our knowledge, there are no reports that demonstrate the use of host molecular markers for the purpose of detecting generic plant virus infection. Two approaches involving molecular indicators of virus infection in the model plant Arabidopsis thaliana were examined: the accumulation of small RNAs (sRNAs) using a microfluidics-based method (Bioanalyzer); and the transcript accumulation of virus-response related host plant genes, suppressor of gene silencing 3 (AtSGS3) and calcium-dependent protein kinase 3 (AtCPK3) by reverse transcriptase-quantitative PCR (RT-qPCR). The microfluidics approach using sRNA chips has previously demonstrated good linearity and good reproducibility, both within and between chips. Good limits of detection have been demonstrated from two-fold 10-point serial dilution regression to 0.1 ng of RNA. The ratio of small RNA (sRNA) to ribosomal RNA (rRNA), as a proportion of averaged mock-inoculation, correlated with known virus infection to a high degree of certainty. AtSGS3 transcript decreased between 14- and 28-days post inoculation (dpi) for all viruses investigated, while AtCPK3 transcript increased between 14 and 28 dpi for all viruses. A combination of these two molecular approaches may be useful for assessment of virus-infection of samples without the need for diagnosis of specific virus infection.
DNA Methylation Changes and Its Associated Genes in Mulberry (Morus alba L.) Yu-711 Response to Drought Stress Using MethylRAD Sequencing
Authors: Ackah Michael , Guo Liangliang , Li Shaocong , Jin Xin , Asakiya Charles , Aboagye Evans Tawiah , Yuan Feng , Wu Mengmeng , Essoh Lionnelle Gyllye , Adjibolosoo Daniel , Attaribo Thomas , Zhang Qiaonan , Qiu Changyu , Lin Qiang , Zhao Weiguo
Published: January 2022

Drought stress remains one of the most detrimental environmental cues affecting plant growth and survival. In this work, the DNA methylome changes in mulberry leaves under drought stress (EG) and control (CK) and their impact on gene regulation were investigated by MethylRAD sequencing. The results show 138,464 (37.37%) and 56,241 (28.81%) methylation at the CG and CWG sites (W = A or T), respectively, in the mulberry genome between drought stress and control. The distribution of the methylome was prevalent in the intergenic, exonic, intronic and downstream regions of the mulberry plant genome. In addition, we discovered 170 DMGs (129 in CG sites and 41 in CWG sites) and 581 DMS (413 in CG sites and 168 in CWG sites). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicates that phenylpropanoid biosynthesis, spliceosome, amino acid biosynthesis, carbon metabolism, RNA transport, plant hormone, signal transduction pathways, and quorum sensing play a crucial role in mulberry response to drought stress. Furthermore, the qRT-PCR analysis indicates that the selected 23 genes enriched in the KEGG pathways are differentially expressed, and 86.96% of the genes share downregulated methylation and 13.04% share upregulation methylation status, indicating the complex link between DNA methylation and gene regulation. This study serves as fundamentals in discovering the epigenomic status and the pathways that will significantly enhance mulberry breeding for adaptation to a wide range of environments.
Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation
Authors: Al-Odayni Abdel-Basit , Alotaibi Dalal H. , Saeed Waseem Sharaf , Al-Kahtani Abdullah , Assiri Ali , Alkhtani Fahad M. , Alrahlah Ali
Published: January 2022

The aim of this study was to evaluate the properties of new dental formulations containing eugenyl-2-hydroxypropyl methacrylate (EgGMA) monomer, as restorative dental material, in terms of their degree of photopolymerization and cytotoxicity. The target model composites (TBEg0, TBEg2.5, and TBEg5) were prepared by mixing 35% organic matrix (TEGDMA/BisGMA (50/50 wt%) of which 0, 2.5, and 5 wt%, respectively, were replaced with EgGMA monomer) with 65% filler (silanized hydroxyapatite (HA)/zinc oxide (ZnO2), 4:3 by weight). The vinylic double-bond conversion (DC) after light-curing was studied using Fourier transform infrared technique whereas cell viability was in vitro tested using primary human gingival fibroblasts cells over 7 days by means of AlamarBlue colorimetric assay. The obtained data were statistically analyzed using ANOVA and Tukey post-hoc tests. The results revealed no significant difference in DC between TBEg2.5 (66.49%) and control (TBEg0; 68.74%), whereas both differ significantly with TBEg5, likely due to the inhibitory effect of eugenol moiety at high concentration. The cell viability test indicated that all the composites are biocompatible. No significant difference was counted between TBEg2.5 and TBEg5, however, both differed significantly from the control (TBEg0). Thus, even though its apparent negative effect on polymerization, EgGMA is potentially safer than bisphenol-derived monomers. Such potential properties may encourage further investigations on term of EgGMA amount optimization, compatibility with other dental resins, and antimicrobial activity.
Characterization and Parametric Study on Mechanical Properties Enhancement in Biodegradable Chitosan-Reinforced Starch-Based Bioplastic Film
Authors: Tan Shiou Xuan , Ong Hwai Chyuan , Andriyana Andri , Lim Steven , Pang Yean Ling , Kusumo Fitranto , Ngoh Gek Cheng
Published: January 2022

Bioplastic has been perceived as a promising candidate to replace petroleum-based plastics due to its environment-friendly and biodegradable characteristics. This study presents the chitosan reinforced starch-based bioplastic film prepared by the solution casting and evaporation method. The effects of processing parameters, i.e., starch concentration, glycerol loading, process temperature and chitosan loading on mechanical properties were examined. Optimum tensile strength of 5.19 MPa and elongation at break of 44.6% were obtained under the combined reaction conditions of 5 wt.% starch concentration, 40 wt.% glycerol loading, 20 wt.% chitosan loading and at a process temperature of 70 °C. From the artificial neural network (ANN) modeling, the coefficient of determination (R2) for tensile strength and elongation at break were found to be 0.9955 and 0.9859, respectively, which proved the model had good fit with the experimental data. Interaction and miscibility between starch and chitosan were proven through the peaks shifting to a lower wavenumber in FTIR and a reduction of crystallinity in XRD. TGA results suggested the chitosan-reinforced starch-based bioplastic possessed reasonable thermal stability under 290 °C. Enhancement in water resistance of chitosan-incorporated starch-based bioplastic film was evidenced with a water uptake of 251% as compared to a 302% registered by the pure starch-based bioplastic film. In addition, the fact that the chitosan-reinforced starch-based bioplastic film degraded to 52.1% of its initial weight after 28 days suggests it is a more sustainable alternative than the petroleum-based plastics.
Correction: Devasahayam, S. Decarbonising the Portland and Other Cements—Via Simultaneous Feedstock Recycling and Carbon Conversions Sans External Catalysts. Polymers 2021, 13, 2462
Authors: Devasahayam Sheila
Published: January 2022

The author wishes to make the following correction to the above paper [...]
Star-Shaped Polydimethylsiloxanes with Organocyclotetrasilsesquioxane Branching-Out Centers: Synthesis and Properties
Authors: Dyuzhikova Yulia , Anisimov Anton , Peregudov Alexander , Buzin Mikhail , Nikiforova Galina , Vasil’ev Viktor , Kostrov Sergey , Buzin Alexander , Stupnikov Alexei , Malakhova Yulia , Shchegolikhina Olga , Muzafarov Aziz
Published: January 2022

New non-crystallizable low-dispersity star-shaped polydimethylsiloxanes (PDMS) containing stereoregular cis-tetra(organo)(dimethylsiloxy)cyclotetrasiloxanes containing methyl-, tolyl- and phenyl-substituents at silicon atoms and the mixture of four stereoisomers of tetra[phenyl(dimethylsiloxy)]cyclotetrasiloxane as the cores were synthesized. Their thermal and viscous properties were studied. All synthesized compounds were characterized by a complex of physicochemical analysis methods: nuclear magnetic resonance (NMR), FT-IR spectroscopy, gel permeation chromatography (GPC), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), viscometry in solution, rheometry, and Langmuir trough study.
Osteoconductive Effect of a Nanocomposite Membrane Treated with UV Radiation
Authors: Olguín Yusser , Acuna-Mendoza Soledad , Otero Carolina , Acevedo Cristian A. , Covarrubias Cristian
Published: January 2022

Modulation of the bio-regenerative characteristics of materials is an indispensable requirement in tissue engineering. Particularly, in bone tissue engineering, the promotion of the osteoconductive phenomenon determines the elemental property of a material be used therapeutically. In addition to the chemical qualities of the constituent materials, the three-dimensional surface structure plays a fundamental role that various methods are expected to modulate in a number of ways, one most promising of which is the use of different types of radiation. In the present manuscript, we demonstrate in a calvarial defect model, that treatment with ultraviolet irradiation allows modification of the osteoconductive characteristics in a biomaterial formed by gelatin and chitosan, together with the inclusion of hydroxyapatite and titanium oxide nanoparticles.
Processing and Quality Control of Masks: A Review
Authors: Farzaneh Sedigheh , Shirinbayan Mohammadali
Published: January 2022

It is clear that viruses, especially COVID-19, can cause infection and injure the human body. These viruses can transfer in different ways, such as in air transfer, which face masks can prevent and reduce. Face masks can protect humans through their filtration function. They include different types and mechanisms of filtration whose performance depends on the texture of the fabric, the latter of which is strongly related to the manufacturing method. Thus, scientists should enrich the information on mask production and quality control by applying a wide variety of tests, such as leakage, dynamic respiratory resistance (DBR), etc. In addition, the primary manufacturing methods (meltblown, spunlaid, drylaid, wetlaid and airlaid) and new additive manufacturing (AM) methods (such as FDM) should be considered. These methods are covered in this study.
Fully Automated Multi-Step Synthesis of Block Copolymers
Authors: Schuett Timo , Kimmig Julian , Zechel Stefan , Schubert Ulrich
Published: January 2022

An automated synthesis protocol is developed for the synthesis of block copolymers in a multi-step approach in a fully automated manner. For this purpose, an automated dialysis setup is combined with robot-based synthesis protocols. Consequently, several block copolymerizations are executed completely automated and compared to the respective manual synthesis. As a result, this study opens up the field of autonomous multi-step reactions without any human interactions.
Theoretical and Experimental Investigation on the 3D Surface Roughness of Material Extrusion Additive Manufacturing Products
Authors: Jiang Shijie , Hu Ke , Zhan Yang , Zhao Chunyu , Li Xiaopeng
Published: January 2022

Material extrusion (ME), one of the most widely used additive manufacturing technique, has the advantages of freedom of design, wide range of raw materials, strong ability to manufacture complex products, etc. However, ME products have obvious surface defects due to the layer-by-layer manufacturing characteristics. To reveal the generation mechanism, the three-dimensional surface roughness (3DSR) of ME products was investigated theoretically and experimentally. Based on the forming process of bonding neck, the 3DSR theoretical model in two different directions (vertical and parallel to the fiber direction) was established respectively. The preparation of ME samples was then completed and a series of experimental tests were performed to determine their surface roughness with the laser microscope. Through the comparison between theoretical and experimental results, the proposed model was validated. In addition, sensitivity analysis is implemented onto the proposed model, investigating how layer thickness, extrusion temperature, and extrusion width influence the samples’ surface roughness. This study provides theoretical basis and technical insight into improving the surface quality of ME products.
Prewetting Induced Hydrophilicity to Augment Photocatalytic Activity of Nanocalcite @ Polyester Fabric
Authors: Qayyum Ayesha , Bhatti Ijaz Ahmad , Ashar Ambreen , Jilani Asim , Iqbal Javed , Mohsin Muhammad , Ishaq Tehmeena , Muhammad Shabbir , Wageh S. , Dustgeer Mohsin Raza
Published: January 2022

To eliminate imidacloprid insecticide from wastewater, nanocalcite was grafted onto the surface of pretreated polyester fabric. The process of seeding was followed by the low temperature hydrothermal method for the growth of nanocalcite for the functionalization of fabric. The goal of this study was to improve the hydrophilicity of the nanocalcite photocatalyst that had been grafted onto the surface of polyester fabric (PF) using acidic and basic prewetting techniques. The morphological characteristics, crystalline nature, surface charge density, functional groups of surface-modified nanocalcite @ PF were determined via SEM, XRD, FTIR, and Zeta potential (ZP), respectively. Characterization results critically disclosed surface roughness due to excessive induction of hydroxyl groups, rhombohedral crystal structure, and high charge density (0.721 mS/cm). Moreover, contact angle of nanocalcite @ PF was calculated to be 137.54° while after acidic and basic prewetting, it was reduced to 87.17° and 48.19°. Similarly, bandgap of the as fabricated nanocalcite was found to be 3.5 eV, while basic prewetted PF showed a reduction in band gap (2.9 eV). The solar photocatalytic mineralization of imidacloprid as a probe pollutant was used to assess the improvement in photocatalytic activity of nanocalcite @ PF after prewetting. Response surface methodology was used to statistically optimize the solar exposure time, concentration of the oxidant, and initial pH of the reaction mixture. Maximum solar photocatalytic degradation of the imidacloprid was achieved by basic prewetted nanocalcite @ PF (up to 91.49%), which was superior to acidic prewetted fabric and as-fabricated nanocalcite @ PF. Furthermore, HPLC and FTIR findings further indicated that imidacloprid was decomposed vastly to harmless species by basic prewetted nanocalcite @ PF.
Characterizing the Tensile Strength of the Fabrics Used in Firefighters’ Bunker Gear under Radiant Heat Exposure
Authors: Mazumder Nur-Us-Shafa , Mandal Sumit , Agnew Robert J. , Petrova Adriana , Boorady Lynn M. , Song Guowen
Published: January 2022

More than 60,000 firefighters’ injuries were reported by the National Fire Protection Association in the U.S. in 2019. Inadequate protection by bunker gear could be a reason for most of the injuries. Firefighters repeatedly encounter thermal hazards due to their job responsibilities. Degradation could occur on bunker gear fabric during thermal exposure. It has been found that the presence of moisture affects performance as well, which may come from wearers’ sweat. Proper evaluation of the tensile strength of the fabrics used in bunker gear could provide information essential for maintenance the overall integrity of the gear. An evaluation of the tensile strength of fabrics when exposed to 10, 15, and 20 kW/m2 radiant heat flux in the presence of moisture is reported. In each fabric system, a total of sixty-four different samples were prepared for four different types of fabric and four levels of moisture which were exposed to three different radiant heat flux for five minutes. Heat flux and moisture levels have significant impact on tensile strength. The effect of moisture on tensile strength in a three-layered fabric system is higher than that for a single layer fabric. An understanding of the impact of heat and moisture on fabric strength has been achieved.
Recovery of Phosphorus in Wastewater in the Form of Polyphosphates: A Review
Authors: Chu Weiran , Shi Yi , Zhang Liang
Published: January 2022

As non-renewable resource, the recovery and utilization of phosphorus from wastewater is an enduring topic. Stimulated by the advances in research on polyphosphates (polyP) as well as the development of Enhanced Biological Phosphorus Removal (EBPR) technology to achieve the efficient accumulation of polyP via polyphosphate accumulating organisms (PAOs), a novel phosphorus removal strategy is considered with promising potential for application in real wastewater treatment processes. This review mainly focuses on the mechanism of phosphorus aggregation in the form of polyP during the phosphate removal process. Further discussion about the reuse of polyP with different chain lengths is provided herein so as to suggest possible application pathways for this biosynthetic product.
Influence of Hydrodynamic Conditions on Precipitation Kinetics of Barium Sulfate in a Multifunctional Reactor
Authors: Małysiak Agata , Walica Tomasz , Fronczyk Tomasz , Lemanowicz Marcin
Published: January 2022

In this paper, the influence of hydrodynamic conditions in Kenics static mixer, which acts as a multifunctional reactor, on precipitation kinetics of barium sulfate is investigated. The investigated range of the Reynolds number varied between 500 and 5000, which covered both laminar and turbulent flow regimes. In all experiments, the relative supersaturation was maintained at the constant level (σ = 205). The obtained precipitate was collected and used for crystal size distribution (CSD) determination. On that basis, the kinetic parameters of the process were calculated using the mixed suspension mixed product removal (MSMPR) mathematical model of the process. It was found that for the whole investigated range of Reynolds number, the mixing conditions were satisfactory. CSD analysis showed that in the laminar regime, a clear tendency in crystal behavior could not be noticed. However, during the analysis of the turbulent regime, the presence of a critical Reynolds number was noticed. Above this value, there is a change in the flow pattern, which results in a change of kinetic parameters (B, G), as well as manifests in a form of a decrease in the value of mean diameters of crystals. The flow pattern change is caused by the geometry of the reactor’s inserts.
Wood Ash Based Treatment of Anaerobic Digestate: State-of-the-Art and Possibilities
Authors: Abelenda Alejandro Moure , Aiouache Farid
Published: January 2022

The problem of current agricultural practices is not limited to land management but also to the unsustainable consumption of essential nutrients for plants, such as phosphorus. This article focuses on the valorization of wood ash and anaerobic digestate for the preparation of a slow-release fertilizer. The underlying chemistry of the blend of these two materials is elucidated by analyzing the applications of the mixture. First, the feasibility of employing low doses (≤1 g total solids (TS) ash/g TS digestate) of wood ash is explained as a way to improve the composition of the feedstock of anaerobic digestion and enhance biogas production. Secondly, a detailed description concerning high doses of wood ash and their uses in the downstream processing of the anaerobic digestate to further enhance its stability is offered. Among all the physico-chemical phenomena involved, sorption processes are meticulously depicted, since they are responsible for nutrient recovery, dewatering, and self-hardening in preparing a granular fertilizer. Simple activation procedures (e.g., carbonization, carbonation, calcination, acidification, wash, milling, and sieving) are proposed to promote immobilization of the nutrients. Due to the limited information on the combined processing of wood ash and the anaerobic digestate, transformations of similar residues are additionally considered. Considering all the possible synergies in the anaerobic digestion and the downstream stages, a dose of ash of 5 g TS ash/g TS digestate is proposed for future experiments.
Enhanced Beetle Antennae Algorithm for Chemical Dynamic Optimization Problems’ Non-Fixed Points Discrete Solution
Authors: Lyu Yucheng , Mo Yuanbin , Lu Yanyue , Liu Rui
Published: January 2022

Dynamic optimization is an important research topic in chemical process control. A dynamic optimization method with good performance can reduce energy consumption and prompt production efficiency. However, the method of solving the problem is complicated in the establishment of the model, and the process of solving the optimal value has a certain degree of difficulty. Based on this, we proposed a non-fixed points discrete method of an enhanced beetle antennae optimization algorithm (EBSO) to solve this kind of problem. Firstly, we converted individual beetles into groups of beetles to search for the best and increase the diversity of the population. Secondly, we introduced a balanced direction strategy, which explored extreme values in new directions before the beetles updated their positions. Finally, a spiral flight mechanism was introduced to change the situation of the beetles flying straight toward the tentacles to prevent the traditional algorithm from easily falling into a certain local range and not being able to jump out. We applied the enhanced algorithm to four classic chemical problems. Meanwhile, we changed the equal time division method or unequal time division method commonly used to solve chemical dynamic optimization problems, and proposed a new interval distribution method—the non-fixed points discrete method, which can more accurately represent the optimal control trajectory. The comparison and analysis of the simulation test results with other algorithms for solving chemical dynamic optimization problems show that the EBSO algorithm has good performance to a certain extent, which further proves the effectiveness of the EBSO algorithm and has a better optimization ability.
Introduction to Special Issue: Study of Microbiological Safety in the Food Chain
Authors: Zdolec Nevijo
Published: January 2022

Ensuring microbiological safety in the food (of animal origin) chain is a challenging task due to the complex interactions among animals, humans and the environment [...]
Modified Activated Carbon for Copper Ion Removal from Aqueous Solution
Authors: Chen Wei-Sheng , Chen Yun-Chung , Lee Cheng-Han
Published: January 2022

Because of increasing environmental awareness, it is becoming more important to remove harmful elements from water solutions. This study used activated carbon (AC) derived from waste wood-based panels as the base material, oxidized with nitric acid (OAC), and grafted with iminodiacetic acid (IDA-OAC) to improve the adsorption capacity and affinity for metals. The characterization of AC, OAC, and IDA-OAC was conducted via FTIR, SEM, N2 adsorption and desorption analysis, elemental analysis, Boehm titration, and point of zero charge (PZC). The instrument studies proved the modified increasing of the functional groups of the adsorbents. Moreover, batch and column experiments were conducted to evaluate the ability of the three adsorbents to remove copper ions from aqueous solution. In batch sorption, IDA-OAC had the highest adsorption capacity (84.51 mg/g) compared to OAC (54.74 mg/g) and AC (24.86 mg/g) at pH 5. The breakthrough point (Ct/Ci = 0.05) of copper ions for IDA-OAC occurred much later than AC in the column experiment (AC = 19 BV, IDA-OAC = 52 BV). The Langmuir isotherm and pseudo-second-model kinetics modeling could better fit with the data obtained from the batch sorption of AC, OAC, and IDA-OAC. The significant capacity and reusability of IDA-OAC displayed high applicability for water treatment.
Influence of Post- and Pre-Acid Treatment during Hydrothermal Carbonization of Sewage Sludge on P-Transformation and the Characteristics of Hydrochar
Authors: Shettigondahalli Ekanthalu Vicky , Narra Satyanarayana , Ender Tommy , Antwi Edward , Nelles Michael
Published: January 2022

Phosphorus (P) recovery from alternative P-rich residues is essential to meet the growing demands of food production globally. Despite sewage sludge being a potential source for P, its direct application on agricultural land is controversial because of the obvious concerns related to heavy metals and organic pollutants. Further, most of the available P recovery and sludge management technologies are cost-intensive as they require mandatory dewatering of sewage sludge. In this regard, hydrothermal carbonization (HTC) has gained great attention as a promising process to effectively treat the wet sewage sludge without it having to be dewatered, and it simultaneously enables the recovery of P. This study was conducted to analyse and compare the influence of acid (H2SO4) addition during and after HTC of sewage sludge on P leaching and the characteristics of hydrochar. The obtained results suggested that despite using the same amount of H2SO4, P leaching from solid to liquid phase was significantly higher when acid was used after the HTC of sewage sludge in comparison with acid utilization during the HTC process. After HTC, the reduction in acid-buffering capacity of sewage sludge and increase in solubility of phosphate precipitating metal ions had a greater influence on the mobilization of P from solid to liquid phase. In contrast, utilization of H2SO4 in different process conditions did not have a great influence on proximate analysis results and calorific value of consequently produced hydrochar.
Christian Accommodative Mindfulness: Definition, Current Research, and Group Protocol
Authors: Garzon Fernando , Benitez-DeVilbiss Andres , Turbessi Vera , Offei Darko Yaa Tiwaa , Berberena Nelsie , Jens Ashley , Wray Kaitlin , Bourne Erica , Keay John , Jenks Jeffrey , Noble Courtney , Artis Carletta
Published: January 2022

More clinicians are using mindfulness-based therapeutic strategies; however, Evangelical Christian clients sometimes worry about the Buddhist origins of these treatments. Christian accommodative mindfulness (CAM) attempts to address these concerns with culturally sensitive adaptations to mindfulness methods. We present a definition of CAM and propose some worldview adjustments to typical mindfulness constructs when working with these clients. The empirical research on Christian-derived meditation strategies and Christian-adapted mindfulness strategies will then be reviewed. We introduce a four-session group CAM protocol currently being researched that focuses on scripture meditation, breath meditation, body awareness, and loving-kindness meditation. Sample scripts are included.
The Mediating Effect of Dispositional Gratitude on the Relationship between Religious Struggles and Self-Esteem: Preliminary Results
Authors: Szcześniak Małgorzata , Falewicz Adam , Madej Daria , Bielecka Grażyna , Pracka Joanna , Rybarski Radosław
Published: January 2022

In comforting or distressing circumstances, individuals tend to have various perceptions of themselves. It seems that religious comfort and religious distress correlate differently with people’s self-esteem. Since the relationship between religiosity and self-esteem is not only direct but can be mediated by other factors that are recognized as buffers against adverse situations, our main goal was to verify whether dispositional gratitude may have an indirect effect on the association between both variables. The research involved data from 254 participants aged 18 to 25 (M = 21.24; SD = 2.09) and included 192 women (76%) and 62 men (24%). To measure the title variables, we used: the Religious Comfort and Strain Scale (RCSS), the Rosenberg Self-Esteem Scale (RSES), and the Gratitude Questionnaire (GQ-6). The results showed that people who consider religion as a source of comfort express positive attitudes toward the self and recognize others’ kindness, as well. In contrast, people who consider religiosity as a cause of fear, stress, and internal strain tend to display a lower subjective sense of personal worth and lower appreciation of the positivity around them. Moreover, gratitude had a mediatory effect on the relationships between religious comfort/negative emotions toward God and self-esteem.
Explaining Aggregated Recovery Rates
Authors: Höcht Stephan , Min Aleksey , Wieczorek Jakub , Zagst Rudi
Published: January 2022

This study on explaining aggregated recovery rates (ARR) is based on the largest existing loss and recovery database for commercial loans provided by Global Credit Data, which includes defaults from 5 continents and over 120 countries. The dependence of monthly ARR from bank loans on various macroeconomic factors is examined and sources of their variability are stated. For the first time, an influence of stochastically estimated monthly growth of GDP USA and Europe is quantified. To extract monthly signals of GDP USA and Europe, dynamic factor models for panel data of different frequency information are employed. Then, the behavior of the ARR is investigated using several regression models with unshifted and shifted explanatory variables in time to improve their forecasting power by taking into account the economic situation after the default. An application of a Markov switching model shows that the distribution of the ARR differs between crisis and prosperity times. The best fit among the compared models is reached by the Markov switching model. Moreover, a significant influence of the estimated monthly growth of GDP in Europe is observed for both crises and prosperity times.