bluuebunny/crossref_metadata_2025 huggingface dataset:
Astrocytes in multiple sclerosis
Authors: Ludwin Samuel K , Rao Vijayaraghava TS , Moore Craig S , Antel Jack P
Published: April 2016

Recent experimental and clinical studies on astrocytes are unraveling the capabilities of these multi-functional cells in normal homeostasis, and in central nervous system (CNS) disease. This review focuses on understanding their behavior in all aspects of the initiation, evolution, and resolution of the multiple sclerosis (MS) lesion. Astrocytes display remarkable flexibility and variability of their physical structure and biochemical output, each aspect finely tuned to the specific stage and location of the disease, participating in both pathogenic and beneficial changes seen in acute and progressive forms. As examples, chemo-attractive or repulsive molecules may facilitate the entry of destructive immune cells but may also aid in the recruitment of oligodendrocyte precursors, essential for repair. Pro-inflammatory cytokines may attack pathogenic cells and also destroy normal oligodendrocytes, myelin, and axons. Protective trophic factors may also open the blood–brain barrier and modulate the extracellular matrix to favor recruitment and persistence of CNS-specific immune cells. A chronic glial scar may confer structural support following tissue loss and inhibit ingress of further noxious insults and also inhibit migration of reparative cells and molecules into the damaged tissue. Continual study into these processes offers the therapeutic opportunities to enhance the beneficial capabilities of these cells while limiting their destructive effects.
Stratification of telehealthcare for patients with chronic obstructive pulmonary disease using a predictive algorithm as decision support: A pilot study
Authors: Stausholm Mads N , Egmose Andreas , Dahl Simon C , Lilholt Pernille H , Cichosz Simon L , Hejlesen Ole K
Published: April 2016

Introduction

The number of patients needing care who suffer from chronic obstructive pulmonary disease (COPD) is expected to increase in the future. The consequences thereof will increase the socio-economic burden for both patients and society. Telehealthcare technologies have shown potential in reducing hospitalisation-related costs and in improving health-related quality of life (HRQOL) for some COPD patients, but not all. The aim of this study was to investigate the potential of predictive algorithms for helping the general practitioner to stratify telehealthcare for COPD patients in a way that maximises HRQOL and minimises COPD-related costs.

Methods

Data from 553 COPD patients based in the North Denmark Region were analysed and used as predictors for four multiple linear regression models. The models were trained and evaluated for their abilities to predict individual patient’s future health- and cost-related developments, with and without telehealthcare.

Results

The average root-mean-square error (RMSE) of the health and cost models was 5.265 HRQOL scores and US dollars (US$)5430.49, respectively. The accuracy regarding the polarity of the predicted changes ranged from 61–65% for the health models and 74–75% for the cost models. While differences in the magnitude of predictions with and without telehealthcare were statistically significant ( p < 0.01), the polarity of predictions was similar across models in 82.05% of all cases.

Discussion

Our results indicate that it may be possible to predict the magnitude and polarity of a COPD patient’s future health- and cost-related developments with and without telehealthcare. Predictive algorithms may provide a useful decision support tool in stratifying telehealthcare for COPD patients.
Responding to international terrorism: The securitisation of the United Kingdom’s ports
Authors: Malcolm James A
Published: April 2016

Illustrates the way in which the pursuit of a ‘war on terror’ in the aftermath of the terrorist attacks in the United States of America on 11 September 2001 framed a policy environment that securitised UK ports. Provides a detailed elaboration of counter-terrorism practice in UK ports in the context of responses to international terrorism, laying out a typology that others interested in port security can work from. Highlights the spatial dimension to the securitisation of UK ports alongside its temporal dimension, noting the pursuit, particularly by the British state and port owners, for greater knowledge about, and through this control over, activities associated with UK ports. Contributes towards efforts to more clearly map and conceptualise maritime security by illustrating the way in which security practice within the shore component of the maritime domain—ports—was justified and practices institutionalised.

This article lays out a typology of counter-terrorism practice in the UK ports in the context of responses to international terrorism, providing a mechanism to examine port security. Focusing on developments in the years after the International Ship and Port Facility (ISPS) Code came into force on 1 July 2004, the article first lays out the policy environment in which counter-terrorism practice was implemented, arguing that the securitisation of UK ports was framed by the pursuit of the ‘war on terror’. With this complete, the article moves to elaborate the typology, examining ‘legislation and regulations’, ‘institutional developments and infrastructure changes’ alongside ‘working practices’ implemented. The article concludes by utilising the typology to argue that the counter-terrorism security response relating to UK ports in this context was constantly evolving, layered and increasingly expansive in scope, highlighting a spatial dimension to the securitisation of UK ports.
Placing the criminalization of school discipline in economic context
Authors: Brent John J
Published: April 2016

The topic of school discipline and punishment has received growing attention. Much of this work explores the rise of exclusion-based policies, increasingly punitive practices, and a buildup of security in schools. Explanations for this often focus on large-scale incidents, the perpetuation of social inequalities, students’ perceived racial/ethnic threat, and shifts in modern governance. Little work, however, has considered the financial aspects influencing schools to adopt criminal justice-based disciplinary practices. This article expands the literature by offering a multilevel investigation that contextualizes a “criminalized school discipline” within economic conditions over the last 30 years. In particular, this article delineates how four economic trends have influenced this trend. These include changes within the postindustrial labor market, federal incentives and markets prioritizing greater school security, tightening financial resources amid budget cuts, and the criminalization of the youth consumer economy. Though prior explanations lend noteworthy explanations for the rise of a punitive disciplinary code in schools, they overlook important economic effects. Investigating these financials conditions will help unpack the complex nature of school discipline while uncovering noteworthy policy implications for how youth are reacted to and punished.
Co-create with stakeholders: Action research approach in service development
Authors: Tossavainen Päivi J
Published: April 2016

This paper promotes the value of including many stakeholders in service development. The experience of co-creative service development is examined through the lens of action research. Engaging multiple stakeholders in face-to-face, in simultaneous joint activities, from various organisations, with different levels of hierarchy, and dissimilar positions, may increase the diversification through the broadness of the information, knowledge, and experiences, and increase the amount, and quality of the development suggestions. This paper is an attempt to tell the story of action research, and how it was applied to increase the understanding of the usefulness of multiple stakeholders in service development. Outcomes of a research project are presented. The paper ends with discussion of the theoretical and practical implications of the conducted research.
Contrast measures for predicting perceptual effects of daylight in architectural renderings
Authors: Rockcastle S , Amundadottir ML , Andersen M
Published: April 2016

Daylit architecture is perceived as a dynamic luminous composition, yet most existing performance metrics were designed to evaluate natural illumination for its ability to adequately illuminate a two-dimensional task surface and avoid glare-based discomfort. It may be argued that task-driven approaches based on surface illumination and glare ignore the likelihood that contrast can provide positive impacts on our visual perception of space. Advances in these metrics to accommodate climate-based sky conditions and occupant behaviour have improved our ability to evaluate task illumination and glare, yet the same attention has not been paid to evaluating positive perceptual responses to daylight. Existing studies have attempted to link subjective ratings of composition to simple global contrast metrics without reaching consensus. More advanced metrics have been developed in computational graphics and vision fields but have not been applied to studies in qualitative lighting research. This paper introduces the results from an online experiment where subject ratings of daylight composition are compared to quantitative contrast measures across a series of renderings. This paper will identify which measures correlate to subjects’ ratings of visual interest and introduces a modified contrast algorithm, which can be used as a novel prediction model for visual interest in daylit renderings.
Violence in youth custody: Risk factors of violent misconduct among inmates of German young offender institutions
Authors: Klatt Thimna , Hagl Stephan , Bergmann Marie C. , Baier Dirk
Published: April 2016

Inmate-on-inmate violence is a serious problem in prisons and young offender institutions. However, most studies on predictors of violent misconduct have focussed on adult inmates. This study examines the perpetration of violence in multiple young offender institutions, using the self-report data of 865 male inmates. Prevalence rates indicate that violence occurs to a high extent in the institutions. Regression analyses show that both importation and deprivation variables significantly predict the perpetration of physical and sexual violence. Specifically, drug use during imprisonment, violent beliefs, and a negative inmate–staff relationship were found to increase the risk of violent misconduct. The implications of these findings are discussed.
Women’s Leadership
Authors: Bierema Laura L.
Published: April 2016

The Problem

Women are well prepared to assume leadership roles—They have the education and the will, yet, they do not conform to gendered organizational images of ideal workers. Women often find themselves in a double bind once they advance into a leadership role: They must be cautious not to appear too masculine or too feminine while also personifying the “ideal” (male) worker by exhibiting masculine behaviors and unwavering commitment to the organization. Holding this line is challenging and often at odds with women’s identity and experienced conflicts between life and work. Our understanding of how best to prepare women for careers and create organizations that are hospitable to them is limited by implicit bias, inadequate learning and development strategies, and cultures resilient to change. Current human resource development (HRD) theory inadequately addresses the issues and challenges women leaders face because most leadership theory is based on privileged White males and highly essentialized.

The Solution

The world is burgeoning with global business, technological innovation, intense competition, and multinational workforces. HRD has a role to play in building effective global business if it can more robustly and broadly address issues related to diversity and inclusion in organizations, particularly the creation of cultures that accept a range of leadership styles and women leaders. It is time to challenge traditional, masculine views of leadership and question how leaders are developed. It is critical to understand women’s leadership if women leaders are to be developed and if persistent gender inequity in organizations is to be addressed.

Stakeholders

Women are obvious stakeholders, but, ultimately, everyone in organizations is a stakeholder benefiting from women leaders and improved leadership, in general. HRD professionals also benefit by understanding better how to develop women leaders, in particular, and leadership, in general. Globally, elected leaders, nongovernmental organizations, and nations can develop policy that has the potential to influence and create educational, occupational, and economic change for women.
Facilitating a School-Based Prevention of Commercial Sexual Exploitation of Children
Authors: Kruger Ann Cale , Harper Erin , Zabek Faith , Collins Staeshe , Perkins Catherine , Meyers Joel
Published: April 2016

The challenges of developing and researching a school-based prevention program using the participatory culture–specific intervention model are described here. We outline the problem of commercial sexual exploitation of children that motivated our project and the characteristics of students most at risk (African American girls in middle school). We provide an analysis of the factors that can facilitate creation and implementation of similar prevention programs in schools. These factors include establishing partnerships with community resources, including school insiders, and establishing trust, especially with students placed at risk.
Change of Community Readiness Over Time
Authors: Åhström Mårten , Larsson Nils , Lindberg Lene
Published: April 2016

Aim. To test the possibility of identifying community readiness changes over time. Method. Key responders in seven municipalities in Stockholm County were interviewed about the availability of alcohol and parental support. The results were analyzed with paired t tests. Changes in community readiness were assessed. Ninety-three key responders from the participating communities were interviewed three times each, resulting in a total of 315 interviews. Data were collected on three occasions separated by ten months from semistructured telephone interviews based on the community readiness model. Findings. Significant readiness changes were found for both issues from baseline to the first follow-up. In terms of the six dimensions of community readiness, significant differences were evident from baseline to the first follow-up for parental support and the reduction of alcohol availability. Apart from knowledge of reduced alcohol availability, there were no significant changes in overall readiness or in the dimensions from the first follow-up to the second. The findings of this study are discussed in relation to earlier studies. Conclusion. In communities with an initial vague awareness of issues, a change in readiness level occurs in less than a year.
Health Promotion Intervention to Improve Diet Quality in Children
Authors: Rosário Rafaela , Araújo Ana , Padrão Patrícia , Lopes Oscar , Moreira André , Pereira Beatriz , Moreira Pedro
Published: April 2016

Objective. This study aims to evaluate the impact of an intervention program, taught by trained teachers, on foods and nutrients components of the Diet Quality Index–International among children in Grades 1 to 4. Method. A total of 464 children (239 female, 6-12 years) from seven elementary Portuguese schools participated in this randomized trial. Three schools were allocated to the intervention, and four to the control group. The intervention program was based on the health promotion model and social cognitive theory. Teachers previously trained by researchers in nutrition, healthy eating, and healthy cooking implemented the intervention in the classroom from November 2008 to March 2009. Sociodemographic, anthropometric, physical activity, and dietary assessments were performed before (2007/2008) and at the end of the intervention (2009). Dietary intake was gathered by a 24-hour dietary recall and the components of Diet Quality Index–International were defined. Results. Children from the intervention schools reported a significantly higher adequacy in vegetable consumption (p = .018) and a significantly higher moderation in sodium consumption (p = .032) compared with the controllers. Conclusion. Our study provides further support for the success of intervention programs that aim to enhance children’s dietary intake. Implementing similar interventions can be promising to support vegetable consumption and moderate sodium intake.
Process Evaluation of the Project SHINE Intervention for African American Families
Authors: St. George Sara M. , Wilson Dawn K. , McDaniel Tyler , Alia Kassandra A.
Published: April 2016

This study describes the process evaluation of Project SHINE, a randomized family-based health promotion intervention that integrated parenting and peer monitoring for improving sedentary behavior, physical activity, and diet in African American families. Adolescent–parent dyads (n = 89) were randomized to a 6-week behavioral, positive parenting, and peer monitoring skills intervention or a general health education comparison condition. Process evaluation included observational ratings of fidelity, attendance records, psychosocial measures, and qualitative interviews. Results indicated that the intervention was delivered with high fidelity based on facilitator adherence (>98% of content delivered) and competent use of theoretically based behavior change and positive parenting skills (100% of ratings >3 on a 1-4 scale). Although only 43% of peers attended the “bring a friend” session, overall attendance was high (4.39 ± 1.51 sessions) as was the retention rate (88%). Parents in the intervention condition reported significant improvements in communication related to adolescents’ engagement in health behaviors both on their own and with peers. These findings were supported by qualitative themes related to improvements in family communication and connectedness. This study provides an innovative example of how future family-based health promotion trials can expand their process evaluation approaches by assessing theoretically relevant positive parenting variables as part of ongoing monitoring.
Predictors for the development of post-thrombotic syndrome in patients with primary lower limb deep venous thrombosis: A case–control study
Authors: Siddiqui Nadeem A , Sophie Ziad , Zafar Farhan , Soares Delvene , Naz Iram
Published: April 2016

Introduction

Post-thrombotic syndrome is a common and debilitating sequelae of lower limb deep venous thrombosis. Very little awareness is present about the risk factors and about the diagnosis, prevention, and treatment of this condition.

Objective

The objective of this study is to identify the predictors of post-thrombotic syndrome after lower limb deep venous thrombosis.

Materials and methods

A case–control study was conducted on all adult patients who were admitted with lower limb deep venous thrombosis at our institution from January 2005 to June 2012. These patients were scheduled for a research clinic visit, which included informed consent, data collection, and physical examination. Patients found to have post-thrombotic syndrome served as cases and those without post-thrombotic syndrome served as controls. Villalta scoring system was used to diagnose the post-thrombotic syndrome and then to assess the severity of the condition in both the groups. Cox regression risk factor analysis was performed to identify the predictors of post-thrombotic syndrome.

Results

Out of the 125 patients examined, 49 were found to have post-thrombotic syndrome. Risk factors found to be significant were body mass index of more than 35 kg/m2 ( n = 13, p = 0.003), history of immobilization ( n = 19, p = 0.003), one or more hypercoagable disorders ( n = 32, p = 0.02), iliofemoral deep venous thrombosis ( n = 18, p = 0.001), complete obstruction on ultrasound ( n = 26, p = 0.016), unstable range of international normalized ratio ( n = 23, p = 0.041) and non-compliance for the use of compressions stockings ( n = 14, p = 0.001). On multivariate analysis, one or more hypercoagable disorder, iliofemoral deep venous thrombosis, and non-compliance to the use of compression stockings were found to be independent risk factors for the development of post-thrombotic syndrome.

Conclusion

One or more hypercoagable disorders, iliofemoral deep venous thrombosis and non-compliance to the use of compression stockings were independent predictors of post-thrombotic syndrome after deep venous thrombosis. These findings will help prognosticate and prevent development of PTS in similar patient populations.
A post hoc evaluation of a sample size re-estimation in the Secondary Prevention of Small Subcortical Strokes study
Authors: McClure Leslie A , Szychowski Jeff M , Benavente Oscar , Hart Robert G , Coffey Christopher S
Published: April 2016

Background/aims:

The use of adaptive designs has been increasing in randomized clinical trials. Sample size re-estimation is a type of adaptation in which nuisance parameters are estimated at an interim point in the trial and the sample size re-computed based on these estimates. The Secondary Prevention of Small Subcortical Strokes study was a randomized clinical trial assessing the impact of single- versus dual-antiplatelet therapy and control of systolic blood pressure to a higher (130–149 mmHg) versus lower (<130 mmHg) target on recurrent stroke risk in a two-by-two factorial design. A sample size re-estimation was performed during the Secondary Prevention of Small Subcortical Strokes study resulting in an increase from the planned sample size of 2500–3020, and we sought to determine the impact of the sample size re-estimation on the study results.

Methods:

We assessed the results of the primary efficacy and safety analyses with the full 3020 patients and compared them to the results that would have been observed had randomization ended with 2500 patients. The primary efficacy outcome considered was recurrent stroke, and the primary safety outcomes were major bleeds and death. We computed incidence rates for the efficacy and safety outcomes and used Cox proportional hazards models to examine the hazard ratios for each of the two treatment interventions (i.e. the antiplatelet and blood pressure interventions).

Results:

In the antiplatelet intervention, the hazard ratio was not materially modified by increasing the sample size, nor did the conclusions regarding the efficacy of mono versus dual-therapy change: there was no difference in the effect of dual- versus monotherapy on the risk of recurrent stroke hazard ratios (n = 3020 HR (95% confidence interval): 0.92 (0.72, 1.2), p = 0.48; n = 2500 HR (95% confidence interval): 1.0 (0.78, 1.3), p = 0.85). With respect to the blood pressure intervention, increasing the sample size resulted in less certainty in the results, as the hazard ratio for higher versus lower systolic blood pressure target approached, but did not achieve, statistical significance with the larger sample (n = 3020 HR (95% confidence interval): 0.81 (0.63, 1.0), p = 0.089; n = 2500 HR (95% confidence interval): 0.89 (0.68, 1.17), p = 0.40). The results from the safety analyses were similar to 3020 and 2500 patients for both study interventions. Other trial-related factors, such as contracts, finances, and study management, were impacted as well.

Conclusion:

Adaptive designs can have benefits in randomized clinical trials, but do not always result in significant findings. The impact of adaptive designs should be measured in terms of both trial results, as well as practical issues related to trial management. More post hoc analyses of study adaptations will lead to better understanding of the balance between the benefits and the costs.
Key influences on special education teachers’ intentions to leave
Authors: Conley Sharon , You Sukkyung
Published: April 2016

The subject of special education teachers’ intentions to leave has been a longstanding concern of researchers and practitioners. This paper reports on a study that examined the workplace predictors of teachers’ intentions to leave for a nationally representative USA sample of 2060 secondary school special education teachers (with students aged approximately 13–18). Structural equation modeling was used to assess the plausibility of a conceptual model, specifying linkages among special education teachers’ perceptions of workplace factors, job satisfaction and commitment, and teachers’ intentions to leave. Using the 2007–2008 Schools and Staffing Survey data set, two models were tested to assess job satisfaction and commitment as mediators. Administrative support and teacher team efficacy had strong, significant direct and indirect effects on special education teachers’ intentions to leave. Teacher job design/autonomy, poor socio-economic/human conditions, and student disengagement also exerted significant indirect effects through satisfaction and commitment. However, administrative support and team efficacy were the most important for these teachers in their effects. Among several demographic variables, teacher age was the most powerful demographic factor, with more experienced teachers having lower intentions to leave. The authors suggest that several conditions and attitudes embedded in the teachers’ responses are influenced by administrators.
Interferon-gamma potentiates NMDA receptor signaling in spinal dorsal horn neurons via microglia–neuron interaction
Authors: Sonekatsu Mayumi , Taniguchi Wataru , Yamanaka Manabu , Nishio Naoko , Tsutsui Shunji , Yamada Hiroshi , Yoshida Munehito , Nakatsuka Terumasa
Published: April 2016

Background

Glia–neuron interactions play an important role in the development of neuropathic pain. Expression of the pro-inflammatory cytokne →cytokine Interferon-gamma (IFNγ) is upregulated in the dorsal horn after peripheral nerve injury, and intrathecal IFNγ administration induces mechanical allodynia in rats. A growing body of evidence suggests that IFNγ might be involved in the mechanisms of neuropathic pain, but its effects on the spinal dorsal horn are unclear. We performed blind whole-cell patch-clamp recording to investigate the effect of IFNγ on postsynaptic glutamate-induced currents in the substantia gelatinosa neurons of spinal cord slices from adult male rats.

Results

IFNγ perfusion significantly enhanced the amplitude of NMDA-induced inward currents in substantia gelatinosa neurons, but did not affect AMPA-induced currents. The facilitation of NMDA-induced current by IFNγ was inhibited by bath application of an IFNγ receptor-selective antagonist. Adding the Janus activated kinase inhibitor tofacitinib to the pipette solution did not affect the IFNγ-induced facilitation of NMDA-induced currents. However, the facilitatory effect of IFNγ on NMDA-induced currents was inhibited by perfusion of the microglial inhibitor minocycline. These results suggest that IFNγ binds the microglial IFNγ receptor and enhances NMDA receptor activity in substantia gelatinosa neurons. Next, to identify the effector of signal transmission from microglia to dorsal horn neurons, we added an inhibitor of G proteins, GDP-β-S, to the pipette solution. In a GDP-β-S–containing pipette solution, IFNγ-induced potentiation of the NMDA current was significantly suppressed after 30 min. In addition, IFNγ-induced potentiation of NMDA currents was blocked by application of a selective antagonist of CCR2, and its ligand CCL2 increased NMDA-induced currents.

Conclusion

Our findings suggest that IFNγ enhance the amplitude of NMDA-induced inward currents in substantia gelatinosa neurons via microglial IFNγ receptors and CCL2/CCR2 signaling. This mechanism might be partially responsible for the development of persistent neuropathic pain.
Visual framing of Muslim women in the Arab Spring: Prominent, active, and visible
Authors: Dastgeer Shugofa , Gade Peter J
Published: April 2016

This content analysis of still images explores how leading media from the US and Middle East (CNN and Al-Jazeera) visually framed Muslim women during the Arab Spring. The findings indicate that when women were in images, they were the primary focal point more than men. Both media framed Muslim women as active participants in the political unrest; however, Al-Jazeera portrayed Muslim women as active significantly more than CNN. The results contrast sharply with previous studies of portrayals of Muslim women in Western media, especially in the post 9/11 era in which women were largely framed as passive victims.
The Medicaid “Free Care” Policy Change
Authors: Lerner Elaine , Edwards Amy
Published: April 2016

On December 15, 2014, the Centers for Medicare and Medicaid Services (CMS) issued guidance to state Medicaid Directors regarding Medicaid Payment for Services Provided without Charge (free care). This guidance may offer local education agencies the opportunity to receive Medicaid reimbursement for health related services when certain requirements are met. This article describes the considerations for local education agencies seeking this reimbursement and the integral role the school nurse could play in the implementation of these requirements.
Colorectal cancer screening: Barriers to the faecal occult blood test (FOBT) and colonoscopy in Singapore
Authors: Yong Sook Kwin , Ong Whee Sze , Koh Gerald Choon-Huat , Yeo Richard Ming Chert , Ha Tam Cam
Published: April 2016

Introduction:

This study aims to identify the barriers to adopting faecal occult blood test (FOBT) and colonoscopy as colorectal cancer (CRC) screening methods among the eligible target population of Singapore.

Materials and methods:

This study was previously part of a randomised controlled trial reported elsewhere. Data was collected from Singapore residents aged 50 and above, via a household sample survey. The study recruited subjects who were aware of CRC screening methods, and interviewed them about the barriers to screening that they faced. Collected results on barriers to each screening method were analysed separately.

Results:

Out of the 343 subjects, 85 (24.8%) recruited knew about FOBT and/or colonoscopy. Most of the respondents (48.9%) cited not having symptoms as the reason for not using the FOBT. This is followed by inconvenience (31.1%), not having any family history of colon cancer (28.9%), lack of time (28.9%) and lack of reminders/recommendation (28.9%). Of the respondents who indicated not choosing colonoscopy as a screening method, more than one-half (54.8%) identified not having any symptoms as the main barrier for them, followed by not having any family history (38.7%) and having a healthy/low-risk lifestyle (29.0%). There was no difference between the reported barriers to each of the screening methods and the respondents’ dwelling types.

Conclusions:

Lack of knowledge, particularly the misconceptions of not having symptoms and being healthy, were identified as the main barriers to FOBT and colonoscopy as screening methods. Interventions to increase the uptake of CRC screening in this population should be tailored to address this misconception.
Mobile phones and political participation in Colombia: Mobile Twitter versus mobile Facebook
Authors: Hopke Jill E. , Gabay Itay , Kim Sojung C , Rojas Hernando
Published: April 2016

In this article, we test the relationships between Twitter and Facebook use on mobile phones and political conversation with offline and online political participation, as well as online expressive communication. Our findings show that using Twitter on mobile phones is associated with a higher likelihood for both online and offline political participation, as well as online expressive communication. Using Facebook is associated with a higher likelihood for online expressive communication only. The key contribution of this article is to show empirical differences between the relationships of social media and social networking on mobile devices with political participation and online expressive communication. Public social media apps, such as Twitter, bring mobile communication back into the public realm of a (albeit diffused) broadcast-like channel. Mobile Twitter adds to the affordances of mobility, networked connectivity, and the publicness of social media.
Dare to Approach
Authors: Enter Dorien , Spinhoven Philip , Roelofs Karin
Published: April 2016

Persistent fear and avoidance in patients with social anxiety disorder (SAD) has been associated with reduced testosterone levels. Because threat avoidance is a major maintaining factor in SAD, and because testosterone administration promotes social approach, we tested whether testosterone administration can directly facilitate threat approach behavior in SAD. In a double-blind, placebo-controlled study, 17 female participants with SAD received a single dose of testosterone before performing a well-established social Approach-Avoidance Task. This objective implicit measure of social motivational action tendencies requires participants to approach or avoid visually presented emotional faces. After testosterone administration, the patients showed increased approach tendencies to angry facial expressions. These results suggest that testosterone can counteract persistent automatic social avoidance tendencies in SAD. This finding advances our understanding of steroid involvement in the regulation of social motivational action in general and in SAD in particular, and may have important clinical implications, promoting testosterone’s candidacy for pharmacological treatment-enhancement studies.
Biologic Treatments for Sports Injuries II Think Tank—Current Concepts, Future Research, and Barriers to Advancement, Part 3
Authors: Zlotnicki Jason P. , Geeslin Andrew G. , Murray Iain R. , Petrigliano Frank A. , LaPrade Robert F. , Mann Barton J. , Musahl Volker
Published: April 2016

Focal chondral defects of the articular surface are a common occurrence in the field of orthopaedics. These isolated cartilage injuries, if not repaired surgically with restoration of articular congruency, may have a high rate of progression to posttraumatic osteoarthritis, resulting in significant morbidity and loss of function in the young, active patient. Both isolated and global joint disease are a difficult entity to treat in the clinical setting given the high amount of stress on weightbearing joints and the limited healing potential of native articular cartilage. Recently, clinical interest has focused on the use of biologically active compounds and surgical techniques to regenerate native cartilage to the articular surface, with the goal of restoring normal joint health and overall function. This article presents a review of the current biologic therapies, as discussed at the 2015 American Orthopaedic Society for Sports Medicine (AOSSM) Biologics Think Tank, that are used in the treatment of focal cartilage deficiencies. For each of these emerging therapies, the theories for application, the present clinical evidence, and specific areas for future research are explored, with focus on the barriers currently faced by clinicians in advancing the success of these therapies in the clinical setting.
Cochlear Implant Rate Pitch and Melody Perception as a Function of Place and Number of Electrodes
Authors: Marimuthu Vijay , Swanson Brett A. , Mannell Robert
Published: April 2016

Six Nucleus cochlear implant recipients participated in a study investigating the effect of place of stimulation on melody perception using rate-pitch cues. Each stimulus was a pulse train delivered on either a single electrode or multiple electrodes sequentially. Four spatial stimulation patterns were used: a single apical electrode, a single mid electrode, a pair of electrodes (apical and mid), and 11 electrodes (from apical to mid). Within one block of trials, all stimuli had the same spatial stimulation pattern, with pulse rate varying from 131 to 262 pps. An additional pulse rate range of 262 to 523 pps was tested with the single-electrode stimuli. Two experimental procedures were used: note ranking; and a modified melodies test with backwards and warp modification. In each trial of the modified melodies test, a familiar melody and a version with modified pitch were presented (in random order), and the subject’s task was to select the unmodified melody. There were no significant differences in performance for stimulation on 1, 2, or 11 electrodes, implying that recipients were unable to combine temporal information from different places in the cochlea to give a stronger pitch cue. No advantage of apical electrodes was found: at the lower pulse rates, there were no significant differences between electrodes; and at the higher pulse rates, scores on the apical electrode dropped more than those on the mid electrode.
Interferon-α signaling promotes embryonic HSC maturation
Authors: Kim Peter Geon , Canver Matthew C. , Rhee Catherine , Ross Samantha J. , Harriss June V. , Tu Ho-Chou , Orkin Stuart H. , Tucker Haley O. , Daley George Q.
Published: April 2016

Key Points

INF-α promotes engraftment of mid-gestation HSCs. INF-α can restore the hematopoietic defect of Arid3a knockout embryos.
UNDR ROVER - a fast and accurate variant caller for targeted DNA sequencing
Authors: Park Daniel J. , Li Roger , Lau Edmund , Georgeson Peter , Nguyen-Dumont Tú , Pope Bernard J.
Published: April 2016

Background

Previously, we described ROVER, a DNA variant caller which identifies genetic variants from PCR-targeted massively parallel sequencing (MPS) datasets generated by the Hi-Plex protocol. ROVER permits stringent filtering of sequencing chemistry-induced errors by requiring reported variants to appear in both reads of overlapping pairs above certain thresholds of occurrence. ROVER was developed in tandem with Hi-Plex and has been used successfully to screen for genetic mutations in the breast cancer predisposition gene PALB2.

ROVER is applied to MPS data in BAM format and, therefore, relies on sequence reads being mapped to a reference genome. In this paper, we describe an improvement to ROVER, called UNDR ROVER (Unmapped primer-Directed ROVER), which accepts MPS data in FASTQ format, avoiding the need for a computationally expensive mapping stage. It does so by taking advantage of the location-specific nature of PCR-targeted MPS data.

Results

The UNDR ROVER algorithm achieves the same stringent variant calling as its predecessor with a significant runtime performance improvement. In one indicative sequencing experiment, UNDR ROVER (in its fastest mode) required 8-fold less sequential computation time than the ROVER pipeline and 13-fold less sequential computation time than a variant calling pipeline based on the popular GATK tool.

UNDR ROVER is implemented in Python and runs on all popular POSIX-like operating systems (Linux, OS X). It requires as input a tab-delimited format file containing primer sequence information, a FASTA format file containing the reference genome sequence, and paired FASTQ files containing sequence reads. Primer sequences at the 5′ end of reads associate read-pairs with their targeted amplicon and, thus, their expected corresponding coordinates in the reference genome. The primer-intervening sequence of each read is compared against the reference sequence from the same location and variants are identified using the same algorithm as ROVER. Specifically, for a variant to be ‘called’ it must appear at the same location in both of the overlapping reads above user-defined thresholds of minimum number of reads and proportion of reads.

Conclusions

UNDR ROVER provides the same rapid and accurate genetic variant calling as its predecessor with greatly reduced computational costs.
BAGEL: a computational framework for identifying essential genes from pooled library screens
Authors: Hart Traver , Moffat Jason
Published: April 2016

Background

The adaptation of the CRISPR-Cas9 system to pooled library gene knockout screens in mammalian cells represents a major technological leap over RNA interference, the prior state of the art. New methods for analyzing the data and evaluating results are needed.

Results

We offer BAGEL (Bayesian Analysis of Gene EssentiaLity), a supervised learning method for analyzing gene knockout screens. Coupled with gold-standard reference sets of essential and nonessential genes, BAGEL offers significantly greater sensitivity than current methods, while computational optimizations reduce runtime by an order of magnitude.

Conclusions

Using BAGEL, we identify ~2000 fitness genes in pooled library knockout screens in human cell lines at 5 % FDR, a major advance over competing platforms. BAGEL shows high sensitivity and specificity even across screens performed by different labs using different libraries and reagents.
Analysis of significant factors for dengue fever incidence prediction
Authors: Siriyasatien Padet , Phumee Atchara , Ongruk Phatsavee , Jampachaisri Katechan , Kesorn Kraisak
Published: April 2016

Background

Many popular dengue forecasting techniques have been used by several researchers to extrapolate dengue incidence rates, including the K-H model, support vector machines (SVM), and artificial neural networks (ANN). The time series analysis methodology, particularly ARIMA and SARIMA, has been increasingly applied to the field of epidemiological research for dengue fever, dengue hemorrhagic fever, and other infectious diseases. The main drawback of these methods is that they do not consider other variables that are associated with the dependent variable. Additionally, new factors correlated to the disease are needed to enhance the prediction accuracy of the model when it is applied to areas of similar climates, where weather factors such as temperature, total rainfall, and humidity are not substantially different. Such drawbacks may consequently lower the predictive power for the outbreak.

Results

The predictive power of the forecasting model-assessed by Akaike’s information criterion (AIC), Bayesian information criterion (BIC), and the mean absolute percentage error (MAPE)-is improved by including the new parameters for dengue outbreak prediction. This study’s selected model outperforms all three other competing models with the lowest AIC, the lowest BIC, and a small MAPE value. The exclusive use of climate factors from similar locations decreases a model’s prediction power. The multivariate Poisson regression, however, effectively forecasts even when climate variables are slightly different. Female mosquitoes and seasons were strongly correlated with dengue cases. Therefore, the dengue incidence trends provided by this model will assist the optimization of dengue prevention.

Conclusions

The present work demonstrates the important roles of female mosquito infection rates from the previous season and climate factors (represented as seasons) in dengue outbreaks. Incorporating these two factors in the model significantly improves the predictive power of dengue hemorrhagic fever forecasting models, as confirmed by AIC, BIC, and MAPE.
Illuminating the physiology of extracellular vesicles
Authors: Choi Hongyoon , Lee Dong Soo
Published: April 2016

Extracellular vesicles play a crucial role in intercellular communication by transmitting biological materials from donor cells to recipient cells. They have pathophysiologic roles in cancer metastasis, neurodegenerative diseases, and inflammation. Extracellular vesicles also show promise as emerging therapeutics, with understanding of their physiology including targeting, distribution, and clearance therefore becoming an important issue. Here, we review recent advances in methods for tracking and imaging extracellular vesicles in vivo and critically discuss their systemic distribution, targeting, and kinetics based on up-to-date evidence in the literature.
Remarks on endpoints of multivalued mappings in geodesic spaces
Authors: Saejung Satit
Published: April 2016

We discuss Panayanak’s results on the existence of an endpoint of a multivalued nonexpansive mapping. We show that all of his results can be extended and some can be established in a wider class of mappings. Out of his three open questions, two of them are solved in affirmative.
The mirn23a microRNA cluster antagonizes B cell development
Authors: Kurkewich Jeffrey L , Bikorimana Emmanuel , Nguyen Tan , Klopfenstein Nathan , Zhang Helen , Hallas William M , Stayback Gwen , McDowell Mary Ann , Dahl Richard
Published: April 2016

Ablation of microRNA synthesis by deletion of the microRNA-processing enzyme Dicer has demonstrated that microRNAs are necessary for normal hematopoietic differentiation and function. However, it is still unclear which specific microRNAs are required for hematopoiesis and at what developmental stages they are necessary. This is especially true for immune cell development. We previously observed that overexpression of the products of the mirn23a gene (microRNA-23a, -24-2, and 27a) in hematopoietic progenitors increased myelopoiesis with a reciprocal decrease in B lymphopoiesis, both in vivo and in vitro. In this study, we generated a microRNA-23a, -24-2, and 27a germline knockout mouse to determine whether microRNA-23a, -24-2, and 27a expression was essential for immune cell development. Characterization of hematopoiesis in microRNA-23a, -24-2, and 27a−/− mice revealed a significant increase in B lymphocytes in both the bone marrow and the spleen, with a concomitant decrease in myeloid cells (monocytes/granulocytes). Analysis of the bone marrow progenitor populations revealed a significant increase in common lymphoid progenitors and a significant decrease in both bone marrow common myeloid progenitors and granulocyte monocyte progenitors. Gene-expression analysis of primary hematopoietic progenitors and multipotent erythroid myeloid lymphoid cells showed that microRNA-23a, -24-2, and 27a regulates essential B cell gene-expression networks. Overexpression of microRNA-24-2 target Tribbles homolog 3 can recapitulate the microRNA-23a, -24-2, and 27a−/− phenotype in vitro, suggesting that increased B cell development in microRNA-23a, -24-2, and 27a null mice can be partially explained by a Tribbles homolog 3-dependent mechanism. Data from microRNA-23a, -24-2, and 27a−/− mice support a critical role for this microRNA cluster in regulating immune cell populations through repression of B lymphopoiesis.
Signatures of breast cancer metastasis at a glance
Authors: Karagiannis George S. , Goswami Sumanta , Jones Joan G. , Oktay Maja H. , Condeelis John S.
Published: April 2016

Gene expression profiling has yielded expression signatures from which prognostic tests can be derived to facilitate clinical decision making in breast cancer patients. Some of these signatures are based on profiling of whole tumor tissue (tissue signatures), which includes all tumor and stromal cells. Prognostic markers have also been derived from the profiling of metastasizing tumor cells, including circulating tumor cells (CTCs) and migratory–disseminating tumor cells within the primary tumor. The metastasis signatures based on CTCs and migratory–disseminating tumor cells have greater potential for unraveling cell biology insights and mechanistic underpinnings of tumor cell dissemination and metastasis. Of clinical interest is the promise that stratification of patients into high or low metastatic risk, as well as assessing the need for cytotoxic therapy, might be improved if prognostics derived from these two types of signatures are used in a combined way. The aim of this Cell Science at a Glance article and accompanying poster is to navigate through both types of signatures and their derived prognostics, as well as to highlight biological insights and clinical applications that could be derived from them, especially when they are used in combination.