bluuebunny/crossref_metadata_2025 huggingface dataset:
Statistical Evaluation of NO2 Emissions in Mashhad City Using Cisco Network Model
Authors: Gheibi Mohammad , Moezzi Reza
Published: September 2024

This paper presents an analysis of NO2 emissions in Mashhad City utilizing statistical evaluations and the Cisco Network Model. The present study begins by evaluating NO2 emissions through statistical analysis, followed by the application of histograms and radar statistical appraisals. Subsequently, a model execution logic is developed using the Cisco Network Model to further understand the distribution and sources of NO2 emissions in the city. Additionally, the research incorporates managerial insights by employing Petri Net modeling, which enables a deeper understanding of the dynamic interactions within the air quality management system. This approach aids in identifying critical control points and optimizing response strategies, thus enhancing the overall effectiveness of urban air pollution mitigation efforts. The findings of this study provide valuable insights into the levels of NO2 pollution in Mashhad City and offer a structured approach to modeling NO2 emissions for effective air quality management strategies which can be extended to the other megacities as well.
Mai kāpae i ke a‘o a ka makua, aia he ola ma laila: Shifting Power through Hawaiian Language Reclamation
Authors: Keli‘ipa‘akaua Justin Kepo‘o , Muneoka Shelley , Braun Kathryn L.
Published: September 2024

Language loss hinders the expression of Indigenous Peoples and their unique worldviews, impairing the intergenerational transfer of knowledge. In Hawai‘i, where a vast majority of the population was fluent and “universally literate” in ‘Ōlelo Hawai‘i from the mid to late 1800s, colonial impositions drastically reduced the number of fluent speakers to roughly 2000 by the 1970s. Efforts to revitalize the language since then have greatly increased the number of current ‘Ōlelo Hawai‘i speakers and resources. Building upon this great work, the Hā Kūpuna National Resource Center for Native Hawaiian Elders at the University of Hawai‘i has initiated projects to contribute to the reclamation of ‘Ōlelo Hawai‘i by increasing our contemporary understanding of ancestral Hawaiian perspectives on elders. To support these projects, significant changes in power structures within our organization were necessary. Insights gained from these projects include gaining clarity on the evolution of the usage of the word “kupuna”, identifying more nuanced perspectives on elders, understanding the importance of family relationships on caregiving outcomes, and understanding the importance of carefully translating English words into ‘Ōlelo Hawai‘i.
Tumor Heterogeneity in Gastrointestinal Cancer Based on Multimodal Data Analysis
Authors: Ai Dongmei , Du Yang , Duan Hongyu , Qi Juan , Wang Yuduo
Published: September 2024

Background: Gastrointestinal cancer cells display both morphology and physiology diversity, thus posing a significant challenge for precise representation by a single data model. We conducted an in-depth study of gastrointestinal cancer heterogeneity by integrating and analyzing data from multiple modalities. Methods: We used a modified Canny algorithm to identify edges from tumor images, capturing intricate nonlinear interactions between pixels. These edge features were then combined with differentially expressed mRNA, miRNA, and immune cell data. Before data integration, we used the K-medoids algorithm to pre-cluster individual data types. The results of pre-clustering were used to construct the kernel matrix. Finally, we applied spectral clustering to the fusion matrix to identify different tumor subtypes. Furthermore, we identified hub genes linked to these subtypes and their biological roles through the application of Weighted Gene Co-expression Network Analysis (WGCNA) and Gene Ontology (GO) enrichment analysis. Results: Our investigation categorized patients into three distinct tumor subtypes and pinpointed hub genes associated with each. Genes MAGI2-AS3, MALAT1, and SPARC were identified as having a differential impact on the metastatic and invasive capabilities of cancer cells. Conclusion: By harnessing multimodal features, our study enhances the understanding of gastrointestinal tumor heterogeneity and identifies biomarkers for personalized medicine and targeted treatments.
Dental Identification System in Public Health: Innovations and Ethical Challenges: A Narrative Review
Authors: Napoletano Gabriele , Putrino Alessandra , Marinelli Enrico , Zaami Simona , De Paola Lina
Published: September 2024

Dental identification systems (DISs) encompass various techniques used for forensic identification, serving as alternatives or complements to genetic methods. Technologies such as microchip implants, prosthetic inscriptions, microSD cards, and identification plaques have been proposed to address limitations in comparative methods, offering streamlined processes for forensic experts. This study reviews current and potential DIS implementations, emphasizing cost-effectiveness and community benefits. Literature analysis from PubMed (2008–2024) yielded 17 relevant articles on implantable DISs, enabling direct subject identification via teeth or prostheses. The integration of DIS aims to enhance accuracy and speed in personal profiling and legal identification, promoting technology transfer in dentistry. It will be necessary to develop strict privacy regulations to protect patient data and establish ethical guidelines for their use. The study’s aim is to highlight that the universal adoption of DISs could mitigate healthcare disputes and facilitate data exchange in clinical settings, which is particularly beneficial for vulnerable populations.
Race, Ethnicity, and Geography as Determinants of Excessive Weight and Low Physical Activity in Pediatric Population: Protocol for Systematic Review and Meta-Analysis
Authors: Statsenko Yauhen , Smetanina Darya , Simiyu Gillian Lylian , Belghali Maroua , Ghenimi Nadirah , Mannaerts Guido Hein Huib , Almaramah Leena , Alhashmi Maryam , Chun Mohammad Nazia , Al Hamed Rahaf , Alblooshi Sara F. , Talbi Khawla , Albreiki Maitha , Alkaabi Fatima , Ponomareva Anna , Ljubisavljevic Milos
Published: September 2024

The rationale for the current study is the sparsity of data on the combined effect of the environmental and individual risks of obesity and sedentary lifestyle in children of different races/ethnicities from different regions. An effective weight management strategy is hard to design due to insufficient evidence. This work was initiated to study race, ethnicity, and geography as determinants of excessive weight and low physical activity in the pediatric population. To achieve this aim, we systematically review publications on daily length of physical activity of light, moderate, and vigorous intensity, as well as sedentary time and BMI and its dynamics in children of different races/ethnicities and geographies. The extracted data are stratified into six major geographic regions and six races/ethnicities. Then, a random-effects meta-analysis is used to calculate the pooled mean of each outcome measure. A ridge regression is constructed to explore age-related change in BMI. A Kruskal–Wallis H test is applied to compare the pooled duration of physical activity and sedentary time in the subgroups. Finally, we calculate paired correlation coefficients between BMI and physical activity/inactivity for each group. The findings can be further used in public health surveillance to clarify the epidemiology of obesity, to guide priority setting and planning, and to develop and evaluate public health policy and strategy.
Measuring the Primary and Secondary Bioreceptivity of Stone and Their Implications for Heritage Conservation
Authors: Skipper Philip J. A. , Skipper Lynda K.
Published: September 2024

Bioreceptivity measures the ability of a surface to develop and maintain a permanent ecosystem of microorganisms. In the historic built environment, this is characterised visually by the greening of monuments and other outdoor heritage. Primary and secondary bioreceptivity refer to the potential for biological growth on fresh and weathered stone, respectively. Measuring bioreceptivity helps us understand biological growth rates and allows researchers to characterise the impact of stone conservation treatments on colonisation. Understanding the relative bioreceptivity of stones allows heritage professionals to make more informed selection of replacement stone types for repairs to existing structures. The primary bioreceptivity of stones from different geographical areas cannot currently be easily compared due to a lack of consistency in approaches to measurement. We propose a repeatable lab-based methodology for measuring colour and chlorophyll a levels from a standard algal culture grown on the stone surface as a standardised testing protocol for primary and secondary bioreceptivity. This protocol controls for the effects of mineralogical colour change during testing, which is something that has not been addressed in other methodologies. This method was successfully applied to case studies measuring the bioreceptivity of English granite, sandstone and limestone, determining their position in a bioreceptivity index. Our results demonstrated that for the stones tested, primary bioreceptivity was categorised as very low or low. Secondary bioreceptivity was increased compared to primary bioreceptivity. This research is the first to fully categorise any stones for secondary bioreceptivity and provides the first primary and secondary bioreceptivity data for any UK stones. We encourage others to follow this standard protocol to add additional data and build an international bioreceptivity database accessible to heritage practitioners.
Discontinuation of Antidepressants and the Risk of Medication Resumption among Community-Dwelling Older Adults with Depression in the US
Authors: Gain Easter P. , Yu Xinhua , Kedia Satish K. , Naser Abu Mohd , Bromley Morgan I. , Ajoku Mark’Quest , Mou Xichen
Published: September 2024

Antidepressants are among the most prescribed drugs in the US, but the current treatment patterns and modalities among older adults are unclear. This study assessed the patterns of discontinuation of antidepressants and the risk of medication resumption among community-dwelling older adults with depression. Using Medicare Current Beneficiary Survey (MCBS) data from 2015–2019, we identified 1084 beneficiaries with depression who newly initiated serotonergic antidepressants. The risk of medication resumption was explored using survival analysis. The median duration of continuous medication was 90 days. However, about 30% of patients had a treatment duration of 30 days or shorter, 26% for 31–90 days, 15% for 91–180 days, and 30% for 181 days or more. After adjusting for all covariates, patients with less than 30 days of continuous medication were half as likely to resume the medications compared to those with 91–180 days (HR: 0.49 (95% CI: 0.37, 0.65)). Nearly one-third of older adults used an antidepressant medication for a short duration with a lower risk of medication resumption. A shorter treatment duration without resumption might suggest over-prescription of antidepressants among community-dwelling older adults.
Construction and Inference Method of Semantic-Driven, Spatio-Temporal Derivation Relationship Network for Place Names
Authors: Dong Wenjie , Mao Xi , Lu Wenjuan , Wang Jizhou , Cheng Yao
Published: September 2024

As the proper noun for geographical entities, place names provide an intuitive way to identify and access specific geographic locations, playing a key role in semantic expression and spatial retrieval. However, existing research has insufficiently explored the spatio-temporal derivation relationships of place names, failing to fully utilize these relationships to enhance the connectivity between place names and improve spatial retrieval capabilities. Therefore, this paper conducts research on the spatio-temporal derivation relationships of place names, defines them in a standardized manner, clarifies the boundary conditions and identification methods, and then constructs a spatio-temporal derivation network of place names for expression and uses this network to carry out reasoning research on spatial adjacency relationships. Experiments and results showed that using the theory and methods of this paper to identify the spatio-temporal derivation relationships of Canadian place names achieves an accuracy rate of 98.5% and a recall rate of 93.4%, and the reasoning results can effectively improve the accuracy of query results. The research enriches the theoretical framework of spatio-temporal derivation relationships of place names, solves the current problems of unclear definition and inability to automatically identify spatio-temporal derivation relationships, and provides new perspectives and tools for the application practice in the field of geographical information science.
Sex-Specific Behavioral and Molecular Responses to Maternal Lipopolysaccharide-Induced Immune Activation in a Murine Model: Implications for Neurodevelopmental Disorders
Authors: Xu Jing , Zhao Rujuan , Yan Mingyang , Zhou Meng , Liu Huanhuan , Wang Xueying , Lu Chang , Li Qiang , Mo Yan , Zhang Paihao , Ju Xingda , Zeng Xianlu
Published: September 2024

Maternal immune activation (MIA) during pregnancy has been increasingly recognized as a critical factor in the development of neurodevelopmental disorders, with potential sex-specific impacts that are not yet fully understood. In this study, we utilized a murine model to explore the behavioral and molecular consequences of MIA induced by lipopolysaccharide (LPS) administration on embryonic day 12.5. Our findings indicate that male offspring exposed to LPS exhibited significant increases in anxiety-like and depression-like behaviors, while female offspring did not show comparable changes. Molecular analyses revealed alterations in pro-inflammatory cytokine levels and synaptic gene expression in male offspring, suggesting that these molecular disruptions may underlie the observed behavioral differences. These results emphasize the importance of considering sex as a biological variable in studies of neurodevelopmental disorders and highlight the need for further molecular investigations to understand the mechanisms driving these sex-specific outcomes. Our study contributes to the growing evidence that prenatal immune challenges play a pivotal role in the etiology of neurodevelopmental disorders and underscores the potential for sex-specific preventative approaches of MIA.
Exposure of Colon-Derived Epithelial Monolayers to Fecal Luminal Factors from Patients with Colon Cancer and Ulcerative Colitis Results in Distinct Gene Expression Patterns
Authors: Magnusson Maria K. , Bas Forsberg Anna , Verveda Alexandra , Sapnara Maria , Lorent Julie , Savolainen Otto , Wettergren Yvonne , Strid Hans , Simrén Magnus , Öhman Lena
Published: September 2024

Microbiota and luminal components may affect epithelial integrity and thus participate in the pathophysiology of colon cancer (CC) and inflammatory bowel disease (IBD). Therefore, we aimed to determine the effects of fecal luminal factors derived from patients with CC and ulcerative colitis (UC) on the colonic epithelium using a standardized colon-derived two-dimensional epithelial monolayer. The complex primary human stem cell-derived intestinal epithelium model, termed RepliGut® Planar, was expanded and passaged in a two-dimensional culture which underwent stimulation for 48 h with fecal supernatants (FS) from CC patients (n = 6), UC patients with active disease (n = 6), and healthy subjects (HS) (n = 6). mRNA sequencing of monolayers was performed and cytokine secretion in the basolateral cell culture compartment was measured. The addition of fecal supernatants did not impair the integrity of the colon-derived epithelial monolayer. However, monolayers stimulated with fecal supernatants from CC patients and UC patients presented distinct gene expression patterns. Comparing UC vs. CC, 29 genes were downregulated and 33 genes were upregulated, for CC vs. HS, 17 genes were downregulated and five genes were upregulated, and for UC vs. HS, three genes were downregulated and one gene was upregulated. The addition of FS increased secretion of IL8 with no difference between the study groups. Fecal luminal factors from CC patients and UC patients induce distinct colonic epithelial gene expression patterns, potentially reflecting the disease pathophysiology. The culture of colonic epithelial monolayers with fecal supernatants derived from patients may facilitate the exploration of IBD- and CC-related intestinal microenvironmental and barrier interactions.
Antibiotic Resistance Hotspot: Comparative Genomics Reveals Multiple Strains of Multidrug-Resistant Citrobacter portucalensis in Edible Snails
Authors: Okafor Arthur C. , Rosel Adriana Cabal , Ogbo Frank C. , Adetunji Charles O. , Imarhiagbe Odoligie , Gamp Lukas , Stöger Anna , Allerberger Franz , Ruppitsch Werner
Published: September 2024

The demand for terrestrial snails as a food source is still on the increase globally, yet this has been overlooked in disease epidemiology and the spread of antimicrobial resistance. This study conducted genomic analyses of twenty Citrobacter portucalensis strains isolated from live edible snails traded in two hubs. The isolates were subjected to MALDI-TOF MS, antimicrobial resistance testing, whole genome sequencing, and analyses for in-depth characterization. The findings disclosed that seventeen strains across the two trading hubs were distinct from previously reported ones. Four isolates were found to share the same sequence type (ST881). Genome-based comparison suggests a clonal transmission of strains between snails traded in these hubs. All the isolates across the two hubs harbored similar variety of antimicrobial resistance genes, with notable ones being blaCMY and qnrB. Sixteen isolates (80%) expressed phenotypic resistance to second-generation cephalosporins, while eleven isolates (55%) exhibited resistance to third-generation cephalosporins. This report of multi-drug-resistant C. portucalensis strains in edible snails highlights significant concerns for food safety and clinical health because of the potential transmission to humans. Enhanced surveillance and stringent monitoring by health authorities are essential to evaluate the impact of these strains on the burden of antimicrobial resistance and to address the associated risk.
The Chronic Toxicity of Endocrine-Disrupting Chemical to Daphnia magna: A Transcriptome and Network Analysis of TNT Exposure
Authors: Lee Jun , Kim Hyun Woo , Shin Dong Yeop , Han Jun Pyo , Jang Yujin , Park Ju Yeon , Yun Seok-Gyu , Cho Eun-Min , Seo Young Rok
Published: September 2024

Endocrine-disrupting chemicals (EDCs) impair growth and development. While EDCs can occur naturally in aquatic ecosystems, they are continuously introduced through anthropogenic activities such as industrial effluents, pharmaceutical production, wastewater, and mining. To elucidate the chronic toxicological effects of endocrine-disrupting chemicals (EDCs) on aquatic organisms, we collected experimental data from a standardized chronic exposure test using Daphnia magna (D. magna), individuals of which were exposed to a potential EDC, trinitrotoluene (TNT). The chronic toxicity effects of this compound were explored through differential gene expression, gene ontology, network construction, and putative adverse outcome pathway (AOP) proposition. Our findings suggest that TNT has detrimental effects on the upstream signaling of Tcf/Lef, potentially adversely impacting oocyte maturation and early development. This study employs diverse bioinformatics approaches to elucidate the gene-level toxicological effects of chronic TNT exposure on aquatic ecosystems. The results provide valuable insights into the molecular mechanisms of the adverse impacts of TNT through network construction and putative AOP proposition.
Metabolomic and Transcriptomic Analyses Reveal the Potential Mechanisms of Dynamic Ovarian Development in Goats during Sexual Maturation
Authors: Wang Yanyan , Chao Tianle , Li Qing , He Peipei , Zhang Lu , Wang Jianmin
Published: September 2024

The ovary is a crucial reproductive organ in mammals, and its development directly influences an individual’s sexual maturity and reproductive capacity. To comprehensively describe ovarian sexual maturation in goats, we integrated phenotypic, hormonal, metabolomic, and transcriptomic data from four specific time points: after birth (D1), at 2 months old (M2), at 4 months old (M4), and at 6 month old (M6). The study showed that during the early stage (D1–M2), ovarian growth was the most rapid, with weight and morphology increasing by 284% and 65%, respectively, and hormone levels rose significantly, with estradiol increasing by 57%. Metabolomic analysis identified 1231 metabolites, primarily lipids, lipid molecules, and organic acids, which can support hormone balance and follicle development by providing energy and participating in signaling transduction. Transcriptomic analysis identified 543 stage-specific differentially expressed genes, mainly enriched in steroid biosynthesis, amino acid metabolism, and the PI3K/AKT pathway, which are key factors influencing ovarian cell proliferation, apoptosis, hormone secretion, and metabolism. The integrated analysis revealed the key processes in the ovarian steroid hormone biosynthesis pathway and gene/metabolite networks associated with ovarian phenotypes and hormone levels, ultimately highlighting scavenger receptor class B type 1 (SCARB1), Cytochrome P450 Family 1 Subfamily A Member 1 (CYP11A1), 3beta-hydroxysteroid dehydrogenase (3BHSD), progesterone, estradiol, and L-phenylalanine as key regulators of ovarian morphological and functional changes at different developmental stages. This study is the first to reveal the metabolic changes and molecular regulatory mechanisms during ovarian sexual maturation in goats, providing valuable insights for understanding reproductive system development and optimizing reproductive performance and breeding efficiency.
Effects of Omega-3 Polyunsaturated Fatty Acids on the Formation of Adipokines, Cytokines, and Oxylipins in Retroperitoneal Adipose Tissue of Mice
Authors: Wenderoth Tatjana , Feldotto Martin , Hernandez Jessica , Schäffer Julia , Leisengang Stephan , Pflieger Fabian , Bredehöft Janne , Mayer Konstantin , Kang Jing , Bier Jens , Grimminger Friedrich , Paßlack Nadine , Rummel Christoph
Published: September 2024

Oxylipins and specialized pro-resolving lipid mediators (SPMs) derived from polyunsaturated fatty acids (PUFAs) are mediators that coordinate an active process of inflammation resolution. While these mediators have potential as circulating biomarkers for several disease states with inflammatory components, the source of plasma oxylipins/SPMs remains a matter of debate but may involve white adipose tissue (WAT). Here, we aimed to investigate to what extent high or low omega (n)-3 PUFA enrichment affects the production of cytokines and adipokines (RT-PCR), as well as oxylipins/SPMs (liquid chromatography–tandem mass spectrometry) in the WAT of mice during lipopolysaccharide (LPS)-induced systemic inflammation (intraperitoneal injection, 2.5 mg/kg, 24 h). For this purpose, n-3 PUFA genetically enriched mice (FAT-1), which endogenously synthesize n-3 PUFAs, were compared to wild-type mice (WT) and combined with n-3 PUFA-sufficient or deficient diets. LPS-induced systemic inflammation resulted in the decreased expression of most adipokines and interleukin-6 in WAT, whereas the n-3-sufficient diet increased them compared to the deficient diet. The n-6 PUFA arachidonic acid was decreased in WAT of FAT-1 mice, while n-3 derived PUFAs (eicosapentaenoic acid, docosahexaenoic acid) and their metabolites (oxylipins/SPMs) were increased in WAT by genetic and nutritional n-3 enrichment. Several oxylipins/SPMs were increased by LPS treatment in WAT compared to PBS-treated controls in genetically n-3 enriched FAT-1 mice. Overall, we show that WAT may significantly contribute to circulating oxylipin production. Moreover, n-3-sufficient or n-3-deficient diets alter adipokine production. The precise interplay between cytokines, adipokines, and oxylipins remains to be further investigated.
Correlating Ultrastructural Changes in the Invasion Area of Colorectal Cancer with CT and MRI Imaging
Authors: Urbaniec-Stompór Joanna , Michalak Maciej , Godlewski Janusz
Published: September 2024

The cancer invasion of the large intestine, a destructive process that begins within the mucous membrane, causes cancer cells to gradually erode specific layers of the intestinal wall. The normal tissues of the intestine are progressively replaced by a tumour mass, leading to the impairment of the large intestine’s proper morphology and function. At the ultrastructural level, the disintegration of the extracellular matrix (ECM) by cancer cells triggers the activation of inflammatory cells (macrophages) and connective tissue cells (myofibroblasts) in this area. This accumulation and the functional interactions between these cells form the tumour microenvironment (TM). The constant modulation of cancer cells and cancer-associated fibroblasts (CAFs) creates a specific milieu akin to non-healing wounds, which induces colon cancer cell proliferation and promotes their survival. This review focuses on the processes occurring at the “front of cancer invasion”, with a particular focus on the role of the desmoplastic reaction in neoplasm development. It then correlates the findings from the microscopic observation of the cancer’s ultrastructure with the potential of modern radiological imaging, such as computer tomography (CT) and magnetic resonance imaging (MRI), which visualizes the tumour, its boundaries, and the tissue reactions in the large intestine.
The Interstitial Gland as a Source of Pro- or Anti-Senescent Cells during Chinchilla Rabbit Ovarian Aging
Authors: Díaz-Hernández Verónica , Marmolejo-Valencia Alejandro , Montiel-De la Cruz César , Piñón-Zárate Gabriela , Montaño Luis M. , Mora-Herrera Silvia Ivonne , Caldelas Ivette
Published: September 2024

The aging ovary in mammals leads to the reduced production of sex hormones and a deterioration in follicle quality. The interstitial gland originates from the hypertrophy of the theca cells of atretic follicles and represents an accumulative structure of the ovary that may contribute to its aging. Here, reproductive and mature rabbit ovaries are used to determine whether the interstitial gland plays a crucial role in ovarian aging. We demonstrate that, in the mature ovary, interstitial gland cells accumulate lipid droplets and show ultrastructural characteristics of lipophagy. Furthermore, they undergo modifications and present a foamy appearance, do not express the pan-leukocyte CD-45 marker, and express CYP11A1. These cells are the first to present an increase in lipofuscin accumulation. In foamy cells, the expression of p21 remains low, PCNA expression is maintained at mature ages, and their nuclei do not show positivity for H2AX. The interstitial gland shows a significant increase in lipofuscin accumulation compared with the ovaries of younger rabbits, but lipofuscin accumulation remains constant at mature ages. Surprisingly, no accumulation of cells with DNA damage is evident, and an increase in proliferative cells is observed at the age of 36 months. We suggest that the interstitial gland initially uses lipophagy to maintain steroidogenic homeostasis and prevent cellular senescence.
Wilson and Jungner Revisited: Are Screening Criteria Fit for the 21st Century?
Authors: Schnabel-Besson Elena , Mütze Ulrike , Dikow Nicola , Hörster Friederike , Morath Marina A. , Alex Karla , Brennenstuhl Heiko , Settegast Sascha , Okun Jürgen G. , Schaaf Christian P. , Winkler Eva C. , Kölker Stefan
Published: September 2024

Driven by technological innovations, newborn screening (NBS) panels have been expanded and the development of genomic NBS pilot programs is rapidly progressing. Decisions on disease selection for NBS are still based on the Wilson and Jungner (WJ) criteria published in 1968. Despite this uniform reference, interpretation of the WJ criteria and actual disease selection for NBS programs are highly variable. A systematic literature search [PubMED search “Wilson” AND “Jungner”; last search 16.07.22] was performed to evaluate the applicability of the WJ criteria for current and future NBS programs and the need for adaptation. By at least two reviewers, 105 publications (systematic literature search, N = 77; manual search, N = 28) were screened for relevant content and, finally, 38 publications were evaluated. Limited by the study design of qualitative text analysis, no statistical evaluation was performed, but a structured collection of reported aspects of criticism and proposed improvements was instead collated. This revealed a set of general limitations of the WJ criteria, such as imprecise terminology, lack of measurability and objectivity, missing pediatric focus, and absent guidance on program management. Furthermore, it unraveled specific aspects of criticism on clinical, diagnostic, therapeutic, and economical aspects. A major obstacle was found to be the incompletely understood natural history and phenotypic diversity of rare diseases prior to NBS implementation, resulting in uncertainty about case definition, risk stratification, and indications for treatment. This gap could be closed through the systematic collection and evaluation of real-world evidence on the quality, safety, and (cost-)effectiveness of NBS, as well as the long-term benefits experienced by screened individuals. An integrated NBS public health program that is designed to continuously learn would fulfil these requirements, and a multi-dimensional framework for future NBS programs integrating medical, ethical, legal, and societal perspectives is overdue.
Impact of Rail Irregularities on Longitudinal Level Deterioration Based on Deconvoluted Data
Authors: Loidolt Markus , Weilguny Roman , Marschnig Stefan
Published: September 2024

When a wheel passes over a rail surface irregularity, the resulting vehicle excitations lead to the application of additional system forces to both the track and the vehicle. These forces contribute to an accelerated track geometry deterioration, which in turn results in increased costs. In a recent paper, a clear correlation between the presence of rail irregularities and poor track geometry quality was demonstrated. Rail surface irregularities thereby were quantified by raw data of a chord-based optical measurement system mounted on the regular track recording vehicle in Austria. This paper deals with deconvolution of the recorded data in order to guarantee irregularity quantification without any distortion. Two different deconvolution approaches are developed and validated by additional measurements. Using the deconvoluted data, previously published evaluations were repeated, and the impact of using deconvoluted data instead of chord values was analysed. The correlation between short-wave effects and track geometry quality can not only be confirmed; it is even stronger than predicted by the chord data. The results of the analysis demonstrate that irregularities with amplitudes exceeding 0.08 mm contribute to an accelerated deterioration in track geometry. Amplitudes of a greater severity result in track geometry levels that are up to 120% inferior to the average.
Indocyanine Green for Assessment of Ureteral Vascularity Can Reduce the Rate of Ureteral Complications in Patients Undergoing Extended Hysterectomy
Authors: Mrugała Maja , Fiutowski Marek , Nowak Krzysztof , Borowiec Zofia , Kasperski Mariusz , Bek Wiktor , Machnicka-Rusek Aneta , Milnerowicz-Nabzdyk Ewa
Published: September 2024

Objectives: This study aimed to evaluate the effectiveness of using indocyanine green (ICG) for assessing ureteral vascularity to reduce ureteral complications in patients undergoing extended hysterectomy for deep endometriosis or oncological indications. Methods: A retrospective-prospective cohort study was conducted at the Centre of Gynecology in Opole, Poland, involving 555 patients who underwent hysterectomy from 2020 to 2023. Patients were categorized based on the Querleu–Morrow classification. ICG was used intraoperatively for vascular assessment in patients with deep endometriosis undergoing wide ureter dissection typical of Type C hysterectomy. Results: Ureteral complications occurred in 12 (2.2%) patients, with a significantly lower complication rate in those who underwent ICG testing (1.7%) compared to those who did not (22.7%, p = 0.001). Prophylactic double-J stenting further reduced the risk of complications. Conclusions: The use of ICG for intraoperative assessment of ureteral vascularity significantly reduces the risk of ureteral complications in complex hysterectomies. Further studies are needed to confirm these findings.
Management Options for Traumatic Posterior Sternoclavicular Joint Dislocation: A Narrative Review with a Single Institution’s Experience
Authors: Ciatti Corrado , Masoni Virginia , Maniscalco Pietro , Asti Chiara , Puma Pagliarello Calogero , Caggiari Gianfilippo , Pes Marco , Rivera Fabrizio , Quattrini Fabrizio
Published: September 2024

Background: Posterior sternoclavicular joint (SCJ) dislocations are rare events that can evolve into real emergencies due to the vital structures in the mediastinum. When closed reduction maneuvers fail, open SCJ reconstruction becomes mandatory, with literature proposing several stabilization techniques that either preserve or remove the SCJ’s mobility. This study is a narrative review of the most recent literature regarding posterior trauma to the SCJ along with a single institution’s experience of this pathology, managed either conservatively or surgically, with a figure-of-eight autologous semitendinosus graft in case of closed reduction failure. Methods: This article provides an overview of posterior traumatic SCJ dislocation, and it describes five cases of patients managed for traumatic posterior SCJ dislocation treated either conservatively or surgically with a figure-of-eight semitendinosus tendon autograft reinforced with high-strength suture tape. A comparison with the most recent literature is performed, focusing on biomechanics. Results: The demographics, the mechanism of injury, the management algorithm and the surgical strategy align with the most recent literature. Despite the final treatment, at one year of follow-up, the ROM was restored with full strength throughout the range of motion of the shoulder with no neurological deficits. The reduced joint successfully healed in imaging, and patients returned to their daily lives. The surgical site wounds and donor harvest sites were perfectly healed. Conclusions: Although recent recommendations for treating posterior traumatic SCJ dislocation have advanced, no universally accepted method of stabilization exists, and the surgical strategy is generally entrusted to the surgeon’s experience. The literature still increasingly supports figure-of-eight ligament reconstruction with a biological or synthetic graft. This work further implements the literature by reporting good outcomes at follow-up.
Anterior Cruciate Ligament Reconstruction Utilizing Double Adjustable-Loop Suspensory Fixation Devices Provides Good Clinical Outcomes in Patients under the Age of 40 Years at Two-Year Follow-Up
Authors: Kyriakidis Theofylaktos , Tzaveas Alexandros , Melas Ioannes , Petras Kosmas , Iosifidou Artemis-Maria , Iosifidis Michael
Published: September 2024

Background: Anterior cruciate ligament reconstruction (ACLR) using double adjustable fixation gained popularity in the last decade due to its minimally invasive technique. However, suspensory fixation devices could be related to recurrent instability, poor clinical outcomes, and patient dissatisfaction. The present study aims to evaluate the clinical outcomes following ACLR using double adjustable-loop suspensory fixation devices in the demanding population of young patients. Methods: Between 2019 and 2022, 95 patients with knee post-traumatic anterior cruciate ligament insufficiency were treated with primary ACLR using semitendinosus quadrupled graft and double adjustable-loop suspensory fixation devices and followed for at least two years. Concomitant lesions were also treated at the same surgical time. The knee examination form of the International Knee Documentation Committee (IKDC) was used to assess clinical evaluation, and the return to physical activities using the Tegner Activity Scale was recorded. Patient-reported objective measures (PROMs) were also evaluated, including the IKDC subjective and Lysholm scores. Results: Sixty-six males and twenty-nine females with a mean age of 23.8 (range 18–37) and a mean BMI of 24.9 (SD ± 2.42) kg/m2 were included in this study. All patients were evaluated clinically as normal or nearly normal at the final follow-up. PROMs also significantly improved postoperatively (p < 0.05) compared to the preoperative values. The Tegner Activity Scale increased from 2 to 7, the IKDC mean score improved from 43.9 (±8.9) to 93.3 (±12.3), and the modified Lysholm from 47.3 (±11.1) to 92.9 (±16.6). No complications or adverse events were recorded. Conclusions: Anterior cruciate ligament reconstruction utilizing double adjustable-loop suspensory fixation devices provides good clinical and functional outcomes in young patients at a two-year follow-up.
Platelet Rich Plasma Therapy in Achilles and Patellar Tendinopathies: Outcomes in Subjects with Diabetes (A Retrospective Case-Control Study)
Authors: Abate Michele , Paganelli Roberto , Pellegrino Raffaello , Di Iorio Angelo , Salini Vincenzo
Published: September 2024

Introduction: Diabetes mellitus (DM) is associated with a high risk of chronic degenerative Achilles (AT) and Patellar (PT) tendinopathies and ruptures. Growth factors (GFs) synthesis in diabetics is substantially decreased in human connective tissues, including in tendons. Platelet Rich Plasma (PRP), which is enriched in GFs, might prove of great help in tendon healing. The aim of the study was to assess whether pre-existent DM or Impaired Glucose Tolerance (IGT) could influence the clinical outcome in subjects undergoing PRP treatment. Methods: Sixty subjects with diabetes/pre-diabetes and sixty euglycemic controls, matched for sex and age, were enrolled. Patients suffering from proximal insertional PT and mid-portion AT, treated with PRP therapy, were included in the study. To assess the basal status and the efficacy of the therapy after 3 and 6 months, the Victorian Institute of Sport Assessment (VISA) questionnaire and the Ultrasound methodology study were used. Patient satisfaction was assessed by means of the Likert Scale. Results: In the population study at 6 months, the mean VISA-score increased (8.92 ± 0.67; p-value < 0.001). The improvement in the diabetic group was less evident compared to the controls (−2.76 ± 0.95; p-value = 0.003). Even though the improvement was poor, it was still significant. MCID analysis revealed that diabetics had higher risk of therapeutic unsuccess. Logistic regression analysis was applied to assess factors associated with unsatisfactory results (Likert-scale) of PRP treatment: AT (O.R.: 3.05; 95%CI: 1.40–6.64; p-value = 0.005), higher BMI values (O.R.: 1.02; 95%CI: 1.01–1.04; p-value = 0.01), and lower VISA score values at baseline (O.R.: 0.95; 95%CI: 0.90–0.99; p-value = 0.04). Conclusions: PRP treatment in AT and PT chronic tendinopathies resulted in less favorable results in subjects with diabetes compared with euglycemic subjects. Moreover, the subjects with PT showed better results than those with AT.
Duration of Dual Antiplatelet Therapy after Percutaneous Coronary Intervention of Unprotected Left Main Coronary Artery Stenosis: 6 versus 12 Months
Authors: Hartikainen Tau Sarra , Mertins Sina , Behrens Max , Neumann Franz-Josef , Valina Christian Marc , Löffelhardt Nikolaus , Rahimi Nedjat Faridun Daniel , Breitbart Philipp , Franke Kilian , Westermann Dirk , Ferenc Miroslaw
Published: September 2024

Background/Objectives: For patients with percutaneous coronary intervention (PCI) of an unprotected left main coronary artery (uLMCA) stenosis, the optimal duration of dual antiplatelet therapy (DAPT) remains a matter of debate. The purpose of this study was to compare clinical outcomes of 6- versus 12-month DAPT duration in patients with PCI of an uLMCA and stable angina. Methods: In this retrospective analysis, we included consecutive patients of our centre who underwent PCI of uLMCA stenosis for stable angina and who received DAPT with acetylsalicylic acid and clopidogrel for either 6 or 12 months. The primary endpoint was the composite of all-cause mortality, myocardial infarction, and target lesion revascularization at one year. Secondary endpoints included individual components of the primary endpoint, definite/probable stent thrombosis, and bleeding. Clinical outcomes were assessed by unadjusted analysis and by inverse probability of treatment weighting (IPTW). Results: Out of 984 included patients, 339 (34.5%) received DAPT for 6 months and 645 (65.5%) for 12 months. The primary endpoint occurred in 51 patients (15.2%) in the 6-month group and in 104 (16.3%) in the 12-month group (p = 0.674). Incidences of stent thrombosis (0.9% versus 0.3%, p = 0.224) and BARC 3,4,5 bleeding (6% versus 5.8%, p = 0.808) were also comparable in both groups. We found no significant differences in the primary endpoint and its components or BARC 3,4,5 bleeding between 6 and 12 months. Conclusions: Our findings do not support the extension of DAPT beyond 6 months after PCI for uLMCA in patients with stable angina.
Composite Building Materials Prepared from Bioresources: Use of Rice Husk for Autoclaved Lightweight Concrete Production
Authors: Peng Shao-Lin , Chen Ying-Liang , Dai Yu-Sheng
Published: September 2024

Rice husk (RH) and straw are common agricultural wastes in Asian countries, and they are potential bioresources for building materials. RH contains a large amount of SiO2, and many studies have burnt RH to ash and then used it as a silica supplement in cement and concrete. However, the combustion of RH has an additional cost and exacerbates CO2 emissions and air pollution. RH inherently has a low bulk density and porous structure; therefore, it should be possible to directly use RH as a lightweight additive in concrete. The purposes of this study were to use RH in the production of autoclaved lightweight concrete (ALC) and to examine the effects of RH on ALC properties. Four RHs with different particle sizes, i.e., >1.2 mm, 0.6–1.2 mm, 0.3–0.6 mm, and <0.3 mm, were used as lightweight additives, and the ALC specimens were prepared with 0–20 wt.% RHs by autoclaving at 189 °C for 12 h. The >0.3 mm RH was applicable to prepare the ALC specimens, and the decomposition effect of <0.3 mm RH was significant. Both the bulk density and the compressive strength of the ALC specimens decreased with increasing RH size. RH with a particle size larger than 1.2 mm seems more appropriate for ALC production than RH with a smaller particle size because of the lower bulk density and higher compressive strength. The Ca/Si ratio decreased with increasing RH size, which affected the formation of tobermorite and thus reduced the compressive strength of the ALC specimens. With a suitable water-to-solid (W/S) ratio, the use of RHs as lightweight additives can yield ALC specimens that meet the requirements of commercial products.
Selective Laser Sintering of Polymers: Process Parameters, Machine Learning Approaches, and Future Directions
Authors: Yehia Hossam M. , Hamada Atef , Sebaey Tamer A. , Abd-Elaziem Walaa
Published: September 2024

Selective laser sintering (SLS) is a bed fusion additive manufacturing technology that facilitates rapid, versatile, intricate, and cost-effective prototype production across various applications. It supports a wide array of thermoplastics, such as polyamides, ABS, polycarbonates, and nylons. However, manufacturing plastic components using SLS poses significant challenges due to issues like low strength, dimensional inaccuracies, and rough surface finishes. The operational principle of SLS involves utilizing a high-power-density laser to fuse polymer or metallic powder surfaces. This paper presents a comprehensive analysis of the SLS process, emphasizing the impact of different processing variables on material properties and the quality of fabricated parts. Additionally, the study explores the application of machine learning (ML) techniques—supervised, unsupervised, and reinforcement learning—in optimizing processes, detecting defects, and ensuring quality control within SLS. The review addresses key challenges associated with integrating ML in SLS, including data availability, model interpretability, and leveraging domain knowledge. It underscores the potential benefits of coupling ML with in situ monitoring systems and closed-loop control strategies to enable real-time adjustments and defect mitigation during manufacturing. Finally, the review outlines future research directions, advocating for collaborative efforts among researchers, industry professionals, and domain experts to unlock ML’s full potential in SLS. This review provides valuable insights and guidance for researchers in regard to 3D printing, highlighting advanced techniques and charting the course for future investigations.
Study on Extraordinarily High-Speed Cutting Mechanics and Its Application to Dry Cutting of Aluminum Alloys with Non-Coated Carbide Tools
Authors: Eto Jun , Hayasaka Takehiro , Shamoto Eiji , Xu Liangji
Published: September 2024

The friction/adhesion between the tool and chip is generally large in metal cutting, and it causes many problems such as high cutting energy/rough surface finish. To suppress this, cutting fluid and tool coating are used in practice, but they are high in energy/cost and environmentally unfriendly. Therefore, this paper investigates the extraordinarily high-speed cutting (EHS cutting) mechanics of mainly soft and highly heat-conductive materials and proposes their application to solve the friction/adhesion problem in an environmentally friendly manner. In order to clarify the EHS cutting mechanics, a simple analytical model is constructed and experiments are conducted with measurement of the cutting temperature and forces. As a result, the following points are clarified/found: (1) heat softening at the secondary plastic deformation zone rather than the primary plastic deformation zone, (2) friction coefficient drop to 0.170 in EHS cutting, and (3) gradually increasing trend of cutting temperature in EHS cutting. Finally, EHS cutting is applied to dry cutting of aluminum alloys with a non-coated carbide tool and compared to conventional wet cutting with a DLC-coated carbide tool, and it is shown that a coating/coolant can be omitted in this region to achieve environmentally friendly cutting.
Seasonal Phase Relationships between Sea Surface Salinity, Surface Freshwater Forcing, and Ocean Surface Processes
Authors: Bingham Frederick M. , Brodnitz Susannah
Published: September 2024

Sea surface salinity (SSS) can change as a result of surface freshwater forcing (FWF) or internal ocean processes such as upwelling or advection. SSS should follow FWF by ¼ cycle, or 3 months, if FWF is the primary process controlling it at the seasonal scale. In this paper, we compare the phase relationship between SSS and FWF (i.e., evaporation minus precipitation over mixed layer depth) over the global (non-Arctic) ocean using in situ SSS and satellite evaporation and precipitation. We found that, instead of the expected 3-month delay between SSS and FWF, the delay is mostly closer to 1–2 months, with SSS peaking too soon relative to FWF. We then computed monthly vertical entrainment and horizontal advection terms of the upper ocean salinity balance equation and added their contributions to the phase of the FWF. The addition of these processes to the seasonal upper ocean salinity balance leads to the phase difference between SSS and the forcing processes being closer to the expected value. We conducted a similar computation with the amplitude of the seasonal SSS and the forcing terms, with less definitive results. The results of this study highlight the important role that ocean processes play in the global freshwater cycle at the seasonal scale.