bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-Hardware-Based Non-Technical Losses Detection Methods: A Review
Authors: Guarda Fernando , Hammerschmitt Bruno , Capeletti Marcelo , Neto Nelson , dos Santos Laura , Prade Lucio , Abaide Alzenira
Published: February 2023

Non-Technical Losses (NTL) represent a serious concern for electric companies. These losses are responsible for revenue losses, as well as reduced system reliability. Part of the revenue loss is charged to legal consumers, thus, causing social imbalance. NTL methods have been developed in order to reduce the impact in physical distribution systems and legal consumers. These methods can be classified as hardware-based and non-hardware-based. Hardware-based methods need an entirely new system infrastructure to be implemented, resulting in high investment and increased cost for energy companies, thus hampering implementation in poorer nations. With this in mind, this paper performs a review of non-hardware-based NTL detection methods. These methods use distribution systems and consumers’ data to detect abnormal energy consumption. They can be classified as network-based, which use network technical parameters to search for energy losses, data-based methods, which use data science and machine learning, and hybrid methods, which combine both. This paper focuses on reviewing non-hardware-based NTL detection methods, presenting a NTL detection methods overview and a literature search and analysis.
Influence of Catalytic Additive Application on the Wood-Based Waste Combustion Process
Authors: Gaze Błażej , Wojtko Paulina , Knutel Bernard , Kobel Przemysław , Bobrowicz Kinga , Bukowski Przemysław , Chojnacki Jerzy , Kielar Jan
Published: February 2023

The furniture industry is one of the most dynamically developing sectors of the Polish economy. Unfortunately, due to national law, it involves producing a significant amount of wood-based waste, which can only be incinerated in installations that meet the requirements for waste incineration or co-incineration plants. This is due to the presence of various types of chemical additives in post-production residues, which increases the emission of hazardous compounds into the atmosphere during combustion. This article presents an analysis of the impact of the use of catalytic additives on the amount of emissions produced from the combustion of wood-based waste. For this purpose, the analyzed material was pelletized by mixing it with the DESONOX catalyst or by spraying the fuel with the DESONOX + H2O solution in a 1:1 ratio. Catalytic substances were introduced into the fuel until a concentration of 0.1% of its mass was obtained. The use of catalysts has significantly reduced CO and NOX emissions into the atmosphere. In the case of carbon monoxide, the most effective was Ad2 (reduction by 44%), and in the case of nitrogen oxides, Ad1 (reduction by 31%) achieved the best outcome. The results from this analysis may be an indication for Polish legislation encouraging furniture plants to burn this waste in their own units using catalytic substances.
The Use of Plants from the Lemnaceae Family for Biofuel Production—A Bibliometric and In-Depth Content Analysis
Authors: Krzywonos Małgorzata , Romanowska-Duda Zdzisława , Seruga Przemysław , Messyasz Beata , Mec Stanisław
Published: February 2023

Plants of the Lemnaceae family are becoming increasingly popular among researchers. The goal of the study was to characterize trends in scientific research related to the use of aquatic plants from the Lemnaceae family for energy purposes, especially for the production of biogas, bioethanol, and other biofuels. These plants fit perfectly into the concept of a circular economy. This study performed a bibliometric and in-depth content analysis to review the use of plants from the Lemnaceae family for biofuel production. A set of 666 articles published from 2008 to 2022 was identified from the Scopus and Web of Science databases. Different analytical scientometric tools (topic mapping and overlay visualization networks) were used to analyze 141 articles; the most influential countries, institutions, authors, journals, and articles were identified. Depth content analysis reveals five research areas: (i) development of duckweed growth and starch accumulation; (ii) development of the pretreatment techniques; (iii) development of ethanol fermentation; (iv) hydrothermal liquefaction and bio-oil production; and (v) anaerobic digestion and biogas production.
Tree Species Mixtures Can Improve the Water Storage of the Litter–Soil Continuum in Subtropical Coniferous Plantations in China
Authors: Ding Ni , Bai Yunxing , Zhou Yunchao
Published: February 2023

Increasing evidence has shown that introducing broadleaved trees into coniferous plantations can regulate hydrologic stores and fluxes; however, the effects and regulatory mechanisms of species mixing on the water conservation capacity of the litter–soil continuum remain poorly understood, and differences among tree species may appear. Herein, we investigated and compared the water conservation capacity of the litter layer (semi-decomposed and decomposed layer) and soil layer (0–100 cm) in a monoculture plantation (Pinus massoniana) and five mixed plantations (Pinus massoniana mixed with Cercidiphyllum japonicum, Manglietia chingii, Camellia oleifera, Michelia maudiae, and Bretschneidera sinensis) and comprehensively considered their potential influencing factors. We discovered that the identity of broadleaved tree species significantly affected the water storage of litter and soil in the mixed plantations (p < 0.05). The effective water-holding capacity of the litter (13.39 t·ha−1) was low due to the coniferous litter’s simple structure and challenging breakdown, despite the fact that the litter stock of the monoculture plantation was substantially larger than that of the mixed plantation (14.72 t·ha−1). Introducing deep-rooted tree species (e.g., Bretschneidera sinensis and Camellia oleifera) into Pinus massoniana farmsteads improved the soil-pore structure and aggregate stability, thereby significantly increasing the 0–100 cm soil water storage. Furthermore, we found that litter storage, soil organic carbon, and litter thickness, as key influencing factors, have complex effects on the water storage of the litter–soil continuum. Generally, these findings demonstrated that mixed plantations can potentially improve the water conservation capacity of the litter–soil system. Nevertheless, special attention should be given to the complementarity between tree species combinations.
Alcohol-Induced Headache with Neuroinflammation: Recent Progress
Authors: Zhu He , Xing Yanxia , Akan Otobong D. , Yang Tao
Published: February 2023

Ethanol and other congeners in alcoholic beverages and foods are known triggers of alcohol-induced headaches (AIHs). Recent studies implicate AIHs as an important underlying factor for neuroinflammation. Studies show the relationship between alcoholic beverages, AIH agents, neuroinflammation, and the pathway they elicit. However, studies elucidating specific AIH agents’ pathways are scarce. Works reviewing their pathways can give invaluable insights into specific substances’ patterns and how they can be controlled. Hence, we reviewed the current understanding of how AIH agents in alcoholic beverages affect neuroinflammation and their specific roles. Ethanol upregulates transient receptor potential cation channel subfamily V member 1 (TRPV1) and Toll-like receptor 4 (TLR4) expression levels; both receptors trigger a neuroinflammation response that promotes AIH manifestation—the most common cause of AIHs. Other congeners such as histamine, 5-HT, and condensed tannins also upregulate TRPV1 and TLR4, neuroinflammatory conditions, and AIHs. Data elucidating AIH agents, associating pathways, and fermentation parameters can help reduce or eliminate AIH inducers and create healthier beverages.
Roe Deer Produce Less Methane and Harbor Distinct Gut Microbiota
Authors: Han Yu , Li Songze , Mu Ruina , Zhao Fei , Yan Xiaogang , Si Huazhe , Li Zhipeng
Published: February 2023

Enteric methane (CH4) is an important greenhouse gas emitted by ruminants. Cervidae produces less CH4 than other ruminants, but the underlying mechanism remains unclear. Here, we measured and compared the gas production, nutrient digestibility, gut microbiota composition, and fermentation characteristics of roe deer (n = 4) and goats (n = 4). After the animals had adapted to the same total mixed ration for 21 days, the gas yield was measured using respiration chambers, and fecal samples were collected. The CH4 yield (g/kg DMI) was significantly lower in roe deer than in goats (p < 0.001), while the difference in carbon dioxide yield was not significant (p > 0.05). Roe deer showed lower digestibility of dry matter (p = 0.005), crude protein (p < 0.001), and neutral detergent fiber (p = 0.02) than goats. Principal coordinate analysis revealed that the bacterial and methanogen communities were significantly different between roe deer and goats, indicating a potential role of host genetics. Roe deer and goats showed enrichment of specific key bacterial and methanogen taxa. The relative abundances of Bifidobacterium, Ruminococcus, Succinivibrio, Treponema, Prevotella, Lachnoclostridium, Christensenellaceae R7, and members of the family Lachnospiraceae were higher in roe deer than in goats (p < 0.05). Methanocorpusculum and Methanobrevibacter were dominant methanogens in the guts of roe deer and goats, respectively, but their species compositions differed significantly between the host species. The predicted metabolic pathways, including those for butyrate and propionate, were significantly more abundant in roe deer than in goats (p < 0.05). The molar proportions of propionate and branched volatile fatty acids were significantly higher in roe deer and goats (p < 0.01), respectively. The variation in CH4 yield was characterized by correlations between digestibility, bacteria and methanogens between roe deer and goats, particularly for members within the taxa Lachnospiraceae and Methanosphaera. In summary, our results revealed that gut bacteria and methanogens differ significantly between high- and low-CH4 emitters and identified microbial taxa potentially involved in the mitigation of CH4 production in ruminants.
Overproduction of Laccase by Trametes versicolor and Pycnoporus sanguineus in Farnesol-Pineapple Waste Solid Fermentation
Authors: Backes Emanueli , Kato Camila Gabriel , de Oliveira Junior Verci Alves , Uber Thaís Marques , dos Santos Luís Felipe Oliva , Corrêa Rúbia Carvalho Gomes , Bracht Adelar , Peralta Rosane Marina
Published: February 2023

The effect of farnesol, a sesquiterpene alcohol, on the production of laccases by Trametes versicolor and Pycnoporus sanguineus in pineapple waste solid-state fermentation was evaluated. Extracellular laccase production reached a maximum of 77.88 ± 5.62 U/g (236% above control) in farnesol-induced cultures of T. versicolor on the 17th day, whereas in a similar P. sanguineus culture, a maximal laccase activity of 130.95 ± 2.20 U/g (159% increase) was obtained on the 17th day. A single 45 KDa laccase was produced by both fungi under the influence of farnesol. These and other data allow us to conclude that farnesol acted as an inducer of the same form of laccase in both fungi. Farnesol disfavored fungal growth by increasing the lag phase, but it also clearly improved the oxidative state of the cultures. Contrary to the results obtained previously in submerged cultures, farnesol did not promote hyperbranching in the fungal mycelia. This is the first demonstration that farnesol is an excellent inducer of laccases in T. versicolor and P. sanguineus in solid-state cultivation. In quantitative terms, the results can be regarded as an excellent starting point for developing industrial or at least pre-industrial procedures to produce laccases using T. versicolor and P sanguineus under the stimulus of farnesol.
Bioconversion of a Lignocellulosic Hydrolysate to Single Cell Oil for Biofuel Production in a Cost-Efficient Fermentation Process
Authors: Rerop Zora S. , Stellner Nikolaus I. , Graban Petra , Haack Martina , Mehlmer Norbert , Masri Mahmoud , Brück Thomas B.
Published: February 2023

Cutaneotrichosporon oleaginosus is a highly efficient single cell oil producer, which in addition to hexoses and pentoses can metabolize organic acids. In this study, fed-batch cultivation with consumption-based acetic acid feeding was further developed to integrate the transformation of an industrial paper mill lignocellulosic hydrolysate (LCH) into yeast oil. Employing pentose-rich LCH as a carbon source instead of glucose significantly improved both biomass formation and lipid titer, reaching 55.73 ± 5.20 g/L and 42.1 ± 1.7 g/L (75.5% lipid per biomass), respectively. This hybrid approach of using acetic acid and LCH in one process was further optimized to increase the share of bioavailable carbon from LCH using a combination of consumption-based and continuous feeding. Finally, the techno-economic analysis revealed a 26% cost reduction when using LCH instead of commercial glucose. In summary, we developed a process leading to a holistic approach to valorizing a pentose-rich industrial waste by converting it into oleochemicals.
Comparison of Aqueous and Lactobacterial-Fermented Mercurialis perennis L. (Dog’s Mercury) Extracts with Respect to Their Immunostimulating Activity
Authors: Lorenz Peter , Zilkowski Ilona , Mailänder Lilo K. , Klaiber Iris , Nicolay Sven , Garcia-Käufer Manuel , Zimmermann-Klemd Amy M. , Turek Claudia , Stintzing Florian C. , Kammerer Dietmar R. , Gründemann Carsten
Published: February 2023

Lactic acid (LA) fermentation of dog’s mercury (M. perennis L.) herbal parts was investigated in samples inoculated with either Lactobacteria (Lactobacillus plantarum and Pediococcus pentosaceus, LBF) or whey (WF). Depending on fermentation time, LA concentrations were monitored in a range of 3.4–15.6 g/L with a concomitant pH decline from 6.5 to 3.9. A broad spectrum of cinnamic acids depsides containing glucaric, malic and 2-hydroxyglutaric acids along with quercetin and kaempferol glycosides were detected by LC-DAD-ESI-MSn. Moreover, in this study novel constituents were also found both in unfermented and fermented extracts. Furthermore, amino acids and particular Lactobacteria metabolites such as biogenic amines (e.g., putrescine, 4-aminobutyric acid, cadaverine) and 5-oxoproline were assigned in WF extracts by GC-MS analysis after silylation. Enhanced NFκB and cytokine expression (IL-6, TNFα, IL-8 and IL-1β) was induced by all extracts, both non-fermented and fermented, in NFκB-THP-1 reporter cells, showing a concentration-dependent immunostimulatory effect. The WF extracts were tested for micronuclei formation in THP-1 cells and toxicity in luminescent bacteria (V. fischeri), whereby no mutagenic or toxic effects could be detected, which corroborates their safe use in pharmaceutical remedies.
Overexpression of a Novel Vacuolar Serine Protease-Encoding Gene (spt1) to Enhance Cellulase Production in Trichoderma Reesei
Authors: Yao Cheng , Sun Ningning , Gao Weihao , Sun Yu , Zhang Jiaxin , Liu Hong , Zhong Yaohua
Published: February 2023

Trichoderma reesei is widely applied as the major industrial fungus for the production of cellulases used for the conversion of lignocellulosic biomass to biofuels and other biobased products. The protein secretion pathway is vital for cellulase secretion, but few reports are related to the role of the vacuole in cellulase production. Here, we identified a novel vacuolar serine protease gene spt1 and investigated the ability of T. reesei to secrete cellulases by disrupting, complementing and overexpressing the spt1 gene. Amino acid sequence analysis of the Spt1 protein showed that it belongs to the subtilisin S8 family and has the conserved catalytic triples (Asp, His, Ser) of the serine protease. The deletion of spt1 did not lead to a decrease in extracellular protease activity, and the observation of mycelia with the Spt1–eGFP fusion expression and the vacuolar membrane dye FM4-64 staining confirmed that Spt1 was an intracellular protease located in the vacuoles of T. reesei. However, the spt1 gene deletion significantly reduced spore production and cellulase secretion, while the spt1 complementation recovered these traits to those of the parental strain. When spt1 was overexpressed by using its native promoter and introducing multiple copies, the cellulase secretion was improved. Furthermore, a strong promoter, Pcdna1, was used to drive the spt1 overexpression, and it was found that the cellulase production was significantly enhanced. Specifically, the filter paper activity of the spt1 overexpression strain SOD-2 reached 1.36 U/mL, which was 1.72 times higher than that of the parental strain. These findings demonstrated that the spt1 gene can be a powerful target for increasing cellulase production in T. reesei, which suggests a possible important role of the vacuole in the cellulase secretion pathway and provides new clues for improving strains for efficient cellulase production.
Improving the Functionality of Lentil–Casein Protein Complexes through Structural Interactions and Water Kefir-Assisted Fermentation
Authors: Alrosan Mohammad , Tan Thuan-Chew , Easa Azhar Mat , Alu’datt Muhammad H. , Tranchant Carole C. , Almajwal Ali Madi , Gammoh Sana , Maghaydah Sofyan , Dheyab Mohammed Ali , Jameel Mahmood S. , Al-Qaisi Ali
Published: February 2023

Highly nutritious lentil proteins (LP) have recently attracted interest in the food industry. However, due to their low solubility, extensive application of LP is severely limited. This study describes a new and successful method for overcoming this challenge by improving the nutritional–functional properties of LP, particularly their solubility and protein quality. By combining protein complexation with water kefir-assisted fermentation, the water solubility of native LP (~58%) increases to over 86% upon the formation of lentil–casein protein complexes (LCPC). Meanwhile, the surface charge increases to over −40 mV, accompanied by alterations in secondary and tertiary structures, as shown by Fourier-transform infrared and UV-vis spectra, respectively. In addition, subjecting the novel LCPC to fermentation increases the protein digestibility from 76% to over 86%, due to the reduction in micronutrients that have some degree of restriction with respect to protein digestibility. This approach could be an effective and practical way of altering plant-based proteins.
A Novel Multipath Transmission Scheme for Information-Centric Networking
Authors: Xu Yong , Ni Hong , Zhu Xiaoyong
Published: February 2023

Due to the overload of IP semantics, the traditional TCP/IP network has a number of problems in scalability, mobility, and security. In this context, information-centric networking (ICN) is proposed to solve these problems. To reduce the cost of deployment and smoothly evolve, the ICN architecture needs to be compatible with existing IP infrastructure. However, the rigid underlying IP routing regulation limits the data transmission efficiency of ICN. In this paper, we propose a novel multipath transmission scheme by utilizing the characteristics and functions of ICN to enhance data transmission. The process of multipath transmission can be regarded as a service, and a multipath transmission service ID (MPSID) is assigned. By using the ICN routers bound to the MPSID as relay nodes, multiple parallel paths between the data source and the receiver are constructed. Moreover, we design a path management mechanism, including path selection and path switching. It can determine the initial path based on historical transmission information and switch to other optimal paths according to the congestion degree during transmission. The experimental results show that our proposed method can improve the average throughput and reduce the average flow completion time and the average chunk completion time.
Nanofibres in Drug Delivery Applications
Authors: Farhaj Samia , Conway Barbara R. , Ghori Muhammad Usman
Published: February 2023

Over the years, scientists have been continually striving to develop innovative solutions to design and fabricate medicines with improved therapeutic potential. Conventional dosage forms, such as tablets, capsules, and injections, are limited when exploited for advanced therapeutics, such as drug targeting. To cater to these limitations, nanofibres have emerged as novel nanomaterials to provide enhanced bioavailability, targeted drug release, extended drug release profile, minimum toxicity, and reduced dosage frequency, which has indisputably improved patient adherence and compliance. This review will concern understanding the potential of drug-loaded nanofibres in drug delivery while comprehending a detailed description of their different production methods. The literature has been thoroughly reviewed to appreciate their potential in developing nanofibrous-based pharmaceutical formulations. Overall, this review has highlighted the importance, versatility, and adaptability of nanofibres in developing medicines with varied drug release kinetics. Several problems must be resolved for their full commercial realisation, such as the drug loading, the initial burst effect, the residual organic solvent, the stability of active agents, and the combined usage of new or existing biocompatible polymers.
Compression of Few-Microjoule Femtosecond Pulses in a Hollow-Core Revolver Fiber
Authors: Losev Leonid , Pazyuk Vladimir , Gladyshev Alexey , Yatsenko Yury , Kosolapov Alexey , Bufetov Igor
Published: February 2023

Gas-filled hollow-core fibers are a convenient tool for laser pulse compression down to a few-cycle duration. The development of compact, efficient and high quality compression schemes for laser pulses of relatively low μJ-level energies is of particular interest. In this work, temporal pulse compression based on nonlinear spectral broadening in a xenon-filled revolver fiber followed by a chirped mirror system is investigated. A 250 fs pulse at a central wavelength of 1.03 μm is compressed to 13.3 fs when the xenon pressure was tuned to provide zero group velocity dispersion near 1.03 μm. The energies of input and compressed pulses are 3.8 and 2.7 μJ, respectively. The compression quality factor of 1.8 is achieved.
Simultaneous Rapid Determination of Seven Alternaria Toxins in Tuberous Crops during Storage Using QuEChERS Coupled with Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry
Authors: Xing Jiali , Wu Xi , Xu Xiaorong , Cheng Hai , Shen Jian , Zheng Ruihang , Mao Lingyan , Luo Xiaohu , Mu Yinghua , Liu Yu
Published: February 2023

Robust and sensitive ultrahigh-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) combined with the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was applied for the detection of seven Alternaria toxins (ATs) in tuberous crops. The influence of tuber conditions (fresh, germinated, and moldy) during storage on the concentration of the seven ATs is also investigated. ATs were extracted with acetonitrile under acidic conditions and purified with a C18 adsorbent. ATs were scanned with electrospray ionization (positive/negative ion) dynamic switching and detected in MRM mode. Calibration curve analysis results reveal good linear relationships in all toxin concentration ranges (R2 > 0.99). The limit of detection and limit of quantification were 0.25–0.70 and 0.83–2.31 μg/kg, respectively. The average recoveries of the seven ATs were 83.2–104% with intra-/inter-day precision at 3.52–6.55% and 4.02–7.26%, respectively. The developed method provided adequate selectivity, sensitivity, and precision in detecting the seven ATs at trace levels, and dispensed with standard addition or matrix-matched calibration to compensate for matrix effects. ATs in the fresh, germinated, and moldy samples of tuberous crops in storage (taro, potato, sweet potato, yam, cassava) were analyzed with this method, and the concentrations were 2.01–14.51 μg/kg and significantly increased with storage duration. ALS was detected in most samples, whereas no quantities of ALT and ATX-I were detected. AME was often detected in combination with AOH in sweet potatoes. TeA and Ten were mostly detected in taro, potato, and yam. The established method could be used for the simultaneous detection and quantification of multicomponent toxins in elaborate matrices.
Green Solvents: Emerging Alternatives for Carotenoid Extraction from Fruit and Vegetable By-Products
Authors: Viñas-Ospino Adriana , López-Malo Daniel , Esteve María José , Frígola Ana , Blesa Jesús
Published: February 2023

Carotenoids have important implications for human health and the food industry due to their antioxidant and functional properties. Their extraction is a crucial step for being able to concentrate them and potentially include them in food products. Traditionally, the extraction of carotenoids is performed using organic solvents that have toxicological effects. Developing greener solvents and techniques for extracting high-value compounds is one of the principles of green chemistry and a challenge for the food industry. This review will analyze the use of green solvents, namely, vegetable oils, supercritical fluids, deep eutectic solvents, ionic liquids, and limonene, combined with nonconventional techniques (ultrasound-assisted extraction and microwave), for carotenoid extraction from fruit and vegetable by-products as upcoming alternatives to organic solvents. Recent developments in the isolation of carotenoids from green solvents and their inclusion in food products will also be discussed. The use of green solvents offers significant advantages in extracting carotenoids, both by decreasing the downstream process of solvent elimination, and the fact that the carotenoids can be included directly in food products without posing a risk to human health.
Fucoidan-Derived Functional Oligosaccharides: Recent Developments, Preparation, and Potential Applications
Authors: Wang Min , Veeraperumal Suresh , Zhong Saiyi , Cheong Kit-Leong
Published: February 2023

Oligosaccharides derived from natural resources are attracting increasing attention as both food and nutraceutical products because of their beneficial health effects and lack of toxicity. During the past few decades, many studies have focused on the potential health benefits of fucoidan. Recently, new interest has emerged in fucoidan, partially hydrolysed into fuco-oligosaccharides (FOSs) or low-molecular weight fucoidan, owing to their superior solubility and biological activities compared with fucoidan. There is considerable interest in their development for use in the functional food, cosmetic, and pharmaceutical industries. Therefore, this review summarises and discusses the preparation of FOSs from fucoidan using mild acid hydrolysis, enzymatic depolymerisation, and radical degradation methods, and discusses the advantages and disadvantages of hydrolysis methods. Several purification steps performed to obtain FOSs (according to the latest reports) are also reviewed. Moreover, the biological activities of FOS that are beneficial to human health are summarised based on evidence from in vitro and in vivo studies, and the possible mechanisms for the prevention or treatment of various diseases are discussed.
Three Molecular Modification Strategies to Improve the Thermostability of Xylanase XynA from Streptomyces rameus L2001
Authors: Zhu Weijia , Qin Liqin , Xu Youqiang , Lu Hongyun , Wu Qiuhua , Li Weiwei , Zhang Chengnan , Li Xiuting
Published: February 2023

Glycoside hydrolase family 11 (GH11) xylanases are the preferred candidates for the production of functional oligosaccharides. However, the low thermostability of natural GH11 xylanases limits their industrial applications. In this study, we investigated the following three strategies to modify the thermostability of xylanase XynA from Streptomyces rameus L2001 mutation to reduce surface entropy, intramolecular disulfide bond construction, and molecular cyclization. Changes in the thermostability of XynA mutants were analyzed using molecular simulations. All mutants showed improved thermostability and catalytic efficiency compared with XynA, except for molecular cyclization. The residual activities of high-entropy amino acid-replacement mutants Q24A and K104A increased from 18.70% to more than 41.23% when kept at 65 °C for 30 min. The catalytic efficiencies of Q24A and K143A increased to 129.99 and 92.26 mL/s/mg, respectively, compared with XynA (62.97 mL/s/mg) when using beechwood xylan as the substrate. The mutant enzyme with disulfide bonds formed between Val3 and Thr30 increased the t1/260 °C by 13.33-fold and the catalytic efficiency by 1.80-fold compared with the wild-type XynA. The high thermostabilities and hydrolytic activities of XynA mutants will be useful for enzymatic production of functional xylo-oligosaccharides.
Microbial Diversity and Flavor Regularity of Soy Milk Fermented Using Kombucha
Authors: Peng Xinhui , Yue Qiang , Chi Qianqi , Liu Yanwei , Tian Tian , Dai Shicheng , Yu Aihua , Wang Shaodong , Wang Huan , Tong Xiaohong , Jiang Lianzhou
Published: February 2023

Plant-based milk is considered a healthy and environmentally sustainable option. However, due to the low protein content of most plant-based milk and the difficulty of gaining flavor acceptance by consumers, its production scale is usually limited. Soy milk is a kind of food with comprehensive nutrition and high protein content. In addition, kombucha is naturally fermented by acetic acid bacteria (AAB), yeast, lactic acid bacteria (LAB), and other microorganisms, and the microorganisms in its system can improve the flavor characteristics of food. In the present study, LAB (commercially purchased) and kombucha were used as fermenting agents for soybean, which was used as a raw material to produce soy milk. A variety of characterization methods were used to study the relationship between the microbial composition and flavor regularity of soy milk produced with different proportions of fermenting agents and different fermentation times. In soy milk produced at 32 °C with a mass ratio of LAB to kombucha of 1:1 and a fermentation time of 42 h, the concentrations of LAB, yeast, and acetic acid bacteria in the milk were optimal at 7.48, 6.68, and 6.83 log CFU/mL, respectively. In fermented soy milk produced with kombucha and LAB, the dominant bacterial genera were Lactobacillus (41.58%) and Acetobacter (42.39%), while the dominant fungal genera were Zygosaccharomyces (38.89%) and Saccharomyces (35.86%). After 42 h, the content of hexanol in the fermentation system of kombucha and LAB decreased from 30.16% to 8.74%, while flavor substances such as 2,5-dimethylbenzaldehyde and linalool were produced. Soy milk fermented with kombucha offers the opportunity to explore the mechanisms associated with flavor formation in multi-strain co-fermentation systems and to develop commercial plant-based fermentation products.
Deep Learning-Based Intelligent Apple Variety Classification System and Model Interpretability Analysis
Authors: Yu Fanqianhui , Lu Tao , Xue Changhu
Published: February 2023

In this study, series networks (AlexNet and VGG-19) and directed acyclic graph (DAG) networks (ResNet-18, ResNet-50, and ResNet-101) with transfer learning were employed to identify and classify 13 classes of apples from 7439 images. Two training datasets, model evaluation metrics, and three visualization methods were used to objectively assess, compare, and interpret five Convolutional Neural Network (CNN)-based models. The results show that the dataset configuration had a significant impact on the classification results, as all models achieved over 96.1% accuracy on dataset A (training-to-testing = 2.4:1.0) compared to 89.4–93.9% accuracy on dataset B (training-to-testing = 1.0:3.7). VGG-19 achieved the highest accuracy of 100.0% on dataset A and 93.9% on dataset B. Moreover, for networks of the same framework, the model size, accuracy, and training and testing times increased as the model depth (number of layers) increased. Furthermore, feature visualization, strongest activations, and local interpretable model-agnostic explanations techniques were used to show the understanding of apple images by different trained models, as well as to reveal how and why the models make classification decisions. These results improve the interpretability and credibility of CNN-based models, which provides guidance for future applications of deep learning methods in agriculture.
Short-Term Hermetic Storage of Wet Maize and Its Effect on Quality
Authors: Yewle Nileshwari Raju , Stroshine Richard L. , Ambrose RP Kingsly , Baributsa Dieudonne
Published: February 2023

Maize is a major crop grown in many regions of the world for human consumption, starch production, and animal feed. After harvest, maize is dried to avoid spoilage caused by fungal growth. However, in the humid tropics, drying maize harvested during the rainy season poses challenges. In such instances, temporary storing maize under hermetic conditions may preserve grain quality while waiting for conditions suitable for drying. Wet maize at the moisture contents (m.c.) of 18, 21, and 24% was stored for up to 21 days in both hermetic and non-hermetic jars. The stored maize was assessed, every 7 days, for germination and related parameters, presence of visible mold, and pH. After 21 days of storage at 18, 21, and 24% m.c., maize germination decreased by 28.5, 25.2, and 95.5 percentage points, respectively, in hermetic jars; and by 28.5, 25.2, and 94.5 percentage points in non-hermetic jars (control). There was visible mold on maize stored in non-hermetic jars after 21 days regardless of m.c. Maize at 21 and 24% m.c. stored in hermetic conditions underwent lactic acid fermentation that reduced the pH. The findings suggest that maize at 18 and 21% m.c. can be stored for 14 and 7 days, respectively, under hermetic conditions without significant loss of quality. Further research is needed to thoroughly assess the application of these findings for temporarily storing and subsequently drying maize on farms and along the grain value chain.
Reduction in the Residues of Penthiopyrad in Processed Edible Vegetables by Various Soaking Treatments and Health Hazard Evaluation in China
Authors: Chang Jinming , Dou Li , Ye Yu , Zhang Kankan
Published: February 2023

Tomato and cucumber are two vital edible vegetables that usually appear in people’s daily diet. Penthiopyrad is a new type of amide chiral fungicide, which is often used for disease control of vegetables (including tomato and cucumber) due to its wide bactericidal spectrum, low toxicity, good penetration, and strong internal absorption. Extensive application of penthiopyrad may have caused potential pollution in the ecosystem. Different processing methods can remove pesticide residues from vegetables and protect human health. In this study, the penthiopyrad removal efficiency of soaking and peeling from tomatoes and cucumbers was evaluated under different conditions. Among different soaking methods, heated water soaking and water soaking with additives (NaCl, acetic acid, and surfactant) presented a more effective reduction ability than other treatments. Due to the specific physicochemical properties of tomatoes and cucumbers, the ultrasound enhances the removal rate of soaking for tomato samples and inhibits it for cucumber samples. Peeling can remove approximately 90% of penthiopyrad from contaminated tomato and cucumber samples. Enantioselectivity was found only during tomato sauce storage, which may be related to the complex microbial community. Health risk assessment data suggests that tomatoes and cucumbers are safer for consumers after soaking and peeling. The results may provide consumers with some useful information to choose better household processing methods to remove penthiopyrad residues from tomatoes, cucumbers, and other edible vegetables.
Green Solvents in the Extraction of Bioactive Compounds from Dried Apple Cultivars
Authors: Hollá Marcela , Pilařová Veronika , Švec František , Sklenářová Hana
Published: February 2023

New extraction protocols, gas-expanded liquid extraction (GXLE), and ultrasound extraction (UE) have been optimized with an emphasis on using green solvents and maximizing the extraction of 14 selected phenolic compounds, including flavonoid-based compounds and phenolic acids from dried apples. The design of the experiments’ approach was applied to optimize the main extraction parameters. Fine tuning included optimization of the flow rate in GXLE and the extraction time for GXLE and UE. Optimized GXLE was carried out with CO2–ethanol–water (34/53.8/12.2; v/v/v) at a flow rate of 3 mL/min at a temperature of 75 °C and pressure of 120 bar for 30 min. UE with ethanol–water 26/74 (v/v) lasted for 10 min at 70 °C. Both methods differed in solvent consumption and sample throughput, while providing a comparable total phenolic content of 2442 µg/g with an RSD < 10% and 2226 µg/g with RSD < 6%, for GXLE and UE, respectively. Both methods were used in determining the phenolic compounds in five apple cultivars, ‘Angold’, ‘Artiga’, ‘Golden Delicious’, ‘Meteor’, and ‘Topaz’. Phenolic profiles were plotted with chlorogenic acid, catechin, epicatechin, hirsutrin, phloridzin, and guaiaverin as the main components. Statistical evaluation, including pair t-test, Bland–Altman test, and linear regression did not reveal any differences between UE and GXLE results.
Chemical Composition, Antioxidant and Antibacterial Activities of Essential Oil Obtained from Chincho (Tagetes elliptica Sm) Leaves Grown in the Peruvian Andes
Authors: Cerrón-Mercado Francis , Perez-Alvarez Jose Angel , Nolazco-Cama Diana , Salva-Ruíz Bettit , Tellez-Monzon Lena , Fernández-López Juana , Viuda-Martos Manuel
Published: February 2023

The chemical composition, antioxidant, and antibacterial properties of the essential oil from the leaves of Tagetes elliptica Sm., grown in Peru, were studied. The EO was extracted using steam distillation, and its chemical composition was analyzed using GC-MS, while the antioxidant activity was evaluated using the radical, scavenging capacity (DPPH and ABTS assays), and ferric reducing antioxidant power (FRAP) assays, ferrous ion chelating (FIC) activity, and the Rancimat test. The antibacterial activity against Staphylococcus aureus, Escherichia coli, and Salmonella infantis was studied using the agar well diffusion method. Twenty-seven compounds were identified in the essential oil, and the major components were cis-tagetenone (37.27%), trans-tagetenone (18.84%), dihydrotagetone (14.38%), and trans-tagetone (5.15%). With regard to antioxidant properties, the IC50 values obtained for the DPPH, ABTS, and FIC assays were 53.37, 46.38, and 22.65 mg/mL, respectively. These values were lower than those obtained for standard butylated hydroxytoluene and ascorbic acid. In the Rancimat test, antioxidant activity was achieved only at high concentration. T. elliptica essential oil showed a marked antibacterial activity against all bacterial strains at all concentrations assayed. This study demonstrated that T. elliptica essential oil could be considered as an alternative to synthetic antioxidants and antimicrobial agents in the food industry.
Dietary Fibre Impacts the Texture of Cooked Whole Grain Rice
Authors: Wattanavanitchakorn Siriluck , Wansuksri Rungtiva , Chaichoompu Ekawat , Kamolsukyeunyong Wintai , Vanavichit Apichart
Published: February 2023

Consumers’ general preference for white rice over whole grain rice stems from the hardness and low palatability of cooked whole grain rice; however, strong links have been found between consuming a large amount of white rice, leading a sedentary lifestyle, and acquiring type 2 diabetes. This led us to formulate a new breeding goal to improve the softness and palatability of whole grain rice while promoting its nutritional value. In this study, the association between dietary fibre profiles (using an enzymatic method combined with high-performance liquid chromatography) and textural properties of whole grain rice (using a texture analyser) was observed. The results showed that a variation in the ratio of soluble dietary fibre (SDF) and insoluble dietary fibre (IDF) influenced the textural characteristics of cooked whole grain rice; found a strong association between SDF to IDF ratio and hardness (r = −0.74, p < 0.01) or gumminess (r = −0.69, p < 0.01) of cooked whole grain rice, and demonstrated that the SDF to IDF ratio was also moderately correlated with cohesiveness (r = −0.45, p < 0.05), chewiness (r = −0.55, p < 0.01), and adhesiveness (r = 0.45, p < 0.05) of cooked whole grain rice. It is suggested that the SDF to IDF ratio can be used as a biomarker for breeding soft and highly palatable whole grain rice of cultivated tropical indica rice to achieve consumer well-being. Lastly, a simple modified method from the alkaline disintegration test was developed for high-throughput screening of dietary fibre profiles in the whole grain indica rice samples.
Study of the Technological Properties of Pedrosillano Chickpea Aquafaba and Its Application in the Production of Egg-Free Baked Meringues
Authors: Fuentes Choya Paula , Combarros-Fuertes Patricia , Abarquero Camino Daniel , Renes Bañuelos Erica , Prieto Gutiérrez Bernardo , Tornadijo Rodríguez María Eugenia , Fresno Baro José María
Published: February 2023

Aquafaba is a by-product derived from legume processing. The aim of this study was to assess the compositional differences and the culinary properties of Pedrosillano chickpea aquafaba prepared with different cooking liquids (water, vegetable broth, meat broth and the covering liquid of canned chickpeas) and to evaluate the sensory characteristics of French-baked meringues made with the different aquafaba samples, using egg white as a control. The content of total solids, protein, fat, ash and carbohydrates of the aquafaba samples were quantified. Foaming and emulsifying capacities, as well as the foam and emulsions stabilities were determined. Instrumental and panel-tester analyses were accomplished to evaluate the sensory characteristics of French-baked meringues. The ingredients added to the cooking liquid and the intensity of the heat treatment affected the aquafaba composition and culinary properties. All types of aquafaba showed good foaming properties and intermediate emulsifying capacities; however, the commercial canned chickpea’s aquafaba was the most similar to egg white. The aquafaba meringues showed less alveoli, greater hardness and fracturability and minimal color changes after baking compared with egg white meringues; the meat and vegetable broth’s aquafaba meringues were the lowest rated by the panel-tester and those prepared with canned aquafaba were the highest scored in the sensory analysis.
Lateral Fractal Formation by Crystallographic Silicon Micromachining
Authors: Kooijman Lucas Johannes , Pordeli Yasser , Berenschot Johan Willem , Tas Niels Roelof
Published: February 2023

A novel wafer-scale silicon fractal fabrication method is presented here for forming pyramids only in the lateral direction using the crystal orientation of silicon. Fractals are fabricated in silicon by masking only the corners (corner lithography) of a cavity in silicon with silicon nitride, where the shape is determined by the crystal {111} planes of the silicon. The octahedral cavity shaped by the {111} planes was previously only used for forming octahedral fractals in all directions, but by using a planar silicon dioxide hard-mask on a silicon (100) wafer, the silicon octahedral cavity is “cut in half”. This creates a pyramid with sharper edges and vertices at its base than those determined by just the {111} planes. This allows selective corner lithography patterning at the vertices of the base while leaving the apex unpatterned, leading to lateral growing of pyramidal fractals. This selective patterning is shown mathematically and then demonstrated by creating a fractal of four generations, with the initial pyramid being 8 µm and the two final generations being of submicron size.
Ultrasensitive and Self-Powered Multiparameter Pressure–Temperature–Humidity Sensor Based on Ultra-Flexible Conductive Silica Aerogel
Authors: He Song , Du Chunhua , Sheng Hongliang , He Chunxiang , Liu Xinyu , Jin Xin , Chen Qilin , Tian Fuliang
Published: February 2023

The application of silica aerogel has been limited because of its poor mechanical properties. In order to expand the application scope of silica aerogel, this study fabricated an ultra-flexible conductive silica aerogel as a multiparameter sensor. The sample is fabricated by introducing poly (3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) on a base of ultra-flexible silica aerogel, which was prepared by a diene synthesis reaction at atmospheric pressure. The pressure, temperature, and humidity can be converted into electrical signals. The pressure sensitivity can reach up to 54.88 kPa−1, and the detection limit is as low as 5 Pa. The temperature resolution is up to 0.1 K, and the response time of humidity is within 4 s. More importantly, the developed multiparameter sensor can be self-powered to realize multiparameter sensing of pressure, temperature, and humidity. The ultra-flexible conductive silica aerogel is a promising candidate for monitoring human activities and fire-affected areas.
Spatial Control over Catalyst Positioning for Increased Micromotor Efficiency
Authors: Keller Shauni , Teora Serena P. , Keskin Arif , Daris Luuk J. C. , Samuels Norman A. P. E. , Boujemaa Moussa , Wilson Daniela A.
Published: February 2023

Motion is influenced by many different aspects of a micromotor’s design, such as shape, roughness and the type of materials used. When designing a motor, asymmetry is the main requirement to take into account, either in shape or in catalyst distribution. It influences both speed and directionality since it dictates the location of propulsion force. Here, we combine asymmetry in shape and asymmetry in catalyst distribution to study the motion of soft micromotors. A microfluidic method is utilized to generate aqueous double emulsions, which upon UV-exposure form asymmetric microgels. Taking advantage of the flexibility of this method, we fabricated micromotors with homogeneous catalyst distribution throughout the microbead and micromotors with different degrees of catalyst localization within the active site. Spatial control over catalyst positioning is advantageous since less enzyme is needed for the same propulsion speed as the homogeneous system and it provides further confinement and compartmentalization of the catalyst. This proof-of-concept of our new design will make the use of enzymes as driving forces for motors more accessible, as well as providing a new route for compartmentalizing enzymes at interfaces without the need for catalyst-specific functionalization.