The Expression of IL-8 in Gingival Rattus novergicus After Exposed by Silver Gel Nano Particles 15 µg/ml
Authors: Widowati Kharinna 
Published: July 2019

<p><strong><em>Background:</em></strong><em> The</em><em> </em><em>uses of silver </em><em>nanoparticle </em><em>are growing so fast, especially in the medical science</em><em>, it’s </em><em>used in many kinds of concentration</em><em>. In dentistry, it’s used </em><em>to decrease halitosis and periodontal diseases, and </em><em>also </em><em>wound healing</em><em> process</em><em>. </em><em>I</em><em>t can affect the viability of the cell</em><em>s, give the bad effects to the human’s health and environment if </em><em>we </em><em>use it in a long-term and in an uncertain concentration. <strong>Purpose</strong></em><strong><em>:</em></strong><em> The aim of the study was to analysis the</em><em> </em><em>healing process of gingival fibroblast in Rattus novergicus after exposed </em><em>by </em><em>15</em><em> </em><em>µg/ml</em><em> </em><em>silver</em><em> gel </em><em>nanoparticles.</em> <strong><em>Materials and Methods:</em></strong><em> This study </em><em>uses </em><em>9 male wistar rats were divided into 3 groups. Group A: Cut (hurt it) in the oral gingiva and exposed to Ag-Np Gel 15 µg/ml for 3 days. After 3 days, they were sacrificed and cut the gingival fibroblast off 3x4 cm size with scalpel. Group B: cut in the oral gingiva and exposed to Ag-Np Gel 15 µg/ml for 5 days. After 5 days they were sacrificed and cut the gingival fibroblast off 3x4 cm size with scalpel. Group C: cut in the oral gingiva and exposed to none for 3 days then cut the gingival fibroblast off 3x4 cm size with scalpel. The expressions of</em><em> IL-8 </em><em>in the </em><em>wound healing </em><em>process were </em><em>painted</em><em> by Im</em><em>m</em><em>unohistochemical </em><em>method</em><em>. This data was analyzed by using the t-test method. <strong>Results:</strong></em><strong><em> </em></strong><em>General characteristic of IL-8</em><em> is </em><em>show</em><em>n in</em><em> brown color im</em><em>m</em><em>unoreactive expression on macrophag</em><em>e</em><em> via immunohistochemic</em><em>a</em><em>l painting with monoclonal antibody anti IL-8</em><em>. </em><em>Mean expression numbers of</em><em> IL-8 </em><em>in the group A = </em><em>11,7</em><em>; group B = 1</em><em>6,3</em><em>; and group C (control) = </em><em>28,3</em><em>. T-test sign.number of group A &amp; C = 0,00</em><em>4</em><em>; group B &amp; C = 0,000. <strong>Conclusion</strong></em><strong><em>:</em></strong><em> The exposure of 15 µg/ml silver gel nanoparticle to gingival fibroblast of Rattus novergicus reduce</em><em>s</em><em> the expressions of</em><em> IL-8 </em><em>in the</em><em> day-3 and day-5 post exposure</em><em>. So, it may accelerate the wound healing process.</em></p><p><strong><em>Keywords:</em></strong><strong><em> </em></strong><em>H</em><em>ealing</em><em>, silver, nanoparticles, </em><em>IL-8</em></p><p><strong><em>Correspondence:</em></strong><em> Kharinna Widowati, </em><em>Department of Science Mouth Disease,</em><em> </em><em>Faculty of Dentistry,</em><em> Hang Tuah</em><em> University,</em><em> </em><em>Arif Rahman Hakim 150</em><em>,</em><em> Surabaya</em><em>,</em><em> </em><em>Email: <span style="text-decoration: underline;"><a href="mailto:kharinna.widowati@gmail.com">kharinna.widowati@gmail.com</a></span></em><em></em></p>