bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Impurities from Sugar Excipient on Filtrate Flux during Ultrafiltration and Diafiltration Process
Authors: Lee Jieun , Na Jiwon , Baek Youngbin
Published: October 2021

Sugar excipients such as sucrose and maltose are widely used for biopharmaceutical formulation to improve protein stability and to ensure isotonicity for administration. However, according to recent literature, pharmaceutical-grade sucrose contained nanoparticulate impurities (NPIs) that result in protein aggregation and degradation. The objective of this study was to evaluate the filtrate flux behavior of sugar solution during ultrafiltration (UF) and diafiltration (DF). Filtrate flux data were obtained using either a tangential flow filtration (TFF) system for DF experiments or a normal flow filtration system for UF experiments. In diafiltration experiments, which were performed using 7 g/L of human immunoglobulin G in a 20 mM histidine buffer with the 100 mM sucrose or maltose, the filtrate flux with sucrose solution decreased significantly. In contrast, the one with maltose solution was in good correspondence with the calculated filtrate flux accounting for the effects of solution viscosity. This large decline in the flux was also observed during UF experiments, in which the presence of NPIs was identified by dynamic light scattering analysis and by capturing an SEM image of the membrane surface after filtration. In addition, highly purified sucrose resulted in a much lower flux decline in TFF in the absence of NPIs. These results provide important insights into the factors governing the optimization of the UF/DF process using appropriate excipients for biopharmaceutical formulation.
Deformation Behavior and Constitutive Equation of 42CrMo Steel at High Temperature
Authors: Liu Hongqiang , Cheng Zhicheng , Yu Wei , Wang Gaotian , Zhou Jie , Cai Qingwu
Published: October 2021

High-temperature reduction pretreatment (HTRP) is a process that can significantly improve the core quality of a billet. The existing flow stress data cannot meet the needs of simulation due to lack of high temperature data. To obtain the hot forming process parameters for the high-temperature reduction pretreatment process of 42CrMo steel, a hot compression experiment of 42CrMo steel was conducted on Gleeble-3500 thermal-mechanical at 1200–1350 °C with the rates of deformation 0.001–10 s−1 and the deformation of 60%, and its deformation behavior at elevated temperature was studied. In this study, the effects of flow stress temperature and strain rate on austenite grain were investigated. Moreover, two typical constitutive models were employed to describe the flow stress, namely the Arrhenius constitutive model of strain compensation and back propagation artificial neural network (BP ANN) model. The performance evaluation shows that BP ANN model has high accuracy and stability to predict the curve. The thermal processing maps under strains of 0.1, 0.2, 0.3, and 0.4 were established. Based on the analysis of the thermal processing map, the optimal high reduction process parameter range of 42CrMo is obtained: the temperature range is 1250–1350 °C, and the strain rate range is 0.01–1 s−1.
Calibration of a Digital Current Transformer Measuring Bridge: Metrological Challenges and Uncertainty Contributions
Authors: Frigo Guglielmo , Agustoni Marco
Published: October 2021

In this paper, we consider the calibration of measuring bridges for non-conventional instrument transformers with digital output. In this context, the main challenge is represented by the necessity of synchronization between analog and digital outputs. To this end, we propose a measurement setup that allows for monitoring and quantifying the main quantities of interest. A possible laboratory implementation is presented and the main sources of uncertainty are discussed. From a metrological point of view, technical specifications and statistical analysis are employed to draw up a rigorous uncertainty budget of the calibration setup. An experimental validation is also provided through the thorough characterization of the measurement accuracy of a commercial device in use at METAS laboratories. The proposed analysis proves how the calibration of measuring bridges for non-conventional instrument transformers requires ad hoc measurement setups and identifies possible space for improvement, particularly in terms of outputs’ synchronization and flexibility of the generation process.
Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System
Authors: Shi Lin , Liu Naiyuan , Liu Gang , Fang Jun
Published: October 2021

Chemicals of emerging concern (CEC) in pig farm breeding wastewater, such as antibiotics, will soon pose a serious threat to public health. It is therefore essential to consider improving the treatment efficiency of piggery wastewater in terms of microorganisms. In order to optimize the overall piggery wastewater treatment system from the perspective of the bacterial community structure and its response to environmental factors, five samples were randomly taken from each area of a piggery’s wastewater treatment system using a random sampling method. The bacterial communities’ composition and their correlation with wastewater quality were then analyzed using Illumina MiSeq high-throughput sequencing. The results showed that the bacterial community composition of each treatment unit was similar. However, differences in abundance were significant, and the bacterial community structure gradually changed with the process. Proteobacteria showed more adaptability to an anaerobic environment than Firmicutes, and the abundance of Tissierella in anaerobic zones was low. The abundance of Clostridial (39.02%) and Bacteroides (20.6%) in the inlet was significantly higher than it was in the aerobic zone and the anoxic zone (p < 0.05). Rhodocyclaceae is a key functional microbial group in a wastewater treatment system, and it is a dominant microbial group in activated sludge. Redundancy analysis (RDA) showed that chemical oxygen demand (COD) had the greatest impact on bacterial community structure. Total phosphorus (TP), total nitrogen (TN), PH and COD contents were significantly negatively correlated with Sphingobacteriia, Betaproteobacteria and Gammaproteobacteria, and significantly positively correlated with Bacteroidia and Clostridia. These results offer basic data and theoretical support for optimizing livestock wastewater treatment systems using bacterial community structures.
Microwave Heating Promotes the S-Alkylation of Aziridine Catalyzed by Molecular Sieves: A Post-Synthetic Approach to Lanthionine-Containing Peptides
Authors: Verdoliva Valentina , Digilio Giuseppe , Saviano Michele , De Luca Stefania
Published: October 2021

Aziridine derivatives involved in nucleophilic ring-opening reactions have attracted great interest, since they allow the preparation of biologically active molecules. A chemoselective and mild procedure to convert a peptide cysteine residue into lanthionine via S-alkylation on aziridine substrates is presented in this paper. The procedure relies on a post-synthetic protocol promoted by molecular sieves to prepare lanthionine-containing peptides and is assisted by microwave irradiation. In addition, it represents a valuable alternative to the stepwise approach, in which the lanthionine precursor is incorporated into peptides as a building block.
Green Fabrication of Zinc Oxide Nanoparticles Using Phlomis Leaf Extract: Characterization and In Vitro Evaluation of Cytotoxicity and Antibacterial Properties
Authors: Alyamani Amal A. , Albukhaty Salim , Aloufi Salman , AlMalki Faizah A. , Al-Karagoly Hassan , Sulaiman Ghassan M.
Published: October 2021

Green nanoparticle synthesis is an environmentally friendly approach that uses natural solvents. It is preferred over chemical and physical techniques due to the time and energy savings. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) through a green method that used Phlomis leaf extract as an effective reducing agent. The synthesis and characterization of ZnO NPs were confirmed by UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Dynamic light scattering (DLS), Zeta potential, and Field Emission Scanning Electron Microscope (FESEM) techniques. In vitro cytotoxicity was determined in L929 normal fibroblast cells using MTT assay. The antibacterial activity of ZnO nanoparticles was investigated using a disk-diffusion method against S. aureus and E. coli, as well as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) content concentrations. XRD results confirmed the nanoparticles’ crystalline structure. Nanoparticle sizes were found to be around 79 nm by FESEM, whereas the hydrodynamic radius of nanoparticles was estimated to be around 165 ± 3 nm by DLS. FTIR spectra revealed the formation of ZnO bonding and surfactant molecule adsorption on the surface of ZnO NPs. It is interesting to observe that aqueous extracts of Phlomis leave plant are efficient reducing agents for green synthesis of ZnO NPs in vitro, with no cytotoxic effect on L929 normal cells and a significant impact on the bacteria tested.
Strategies to Investigate Membrane Damage, Nucleoid Condensation, and RNase Activity of Bacterial Toxin–Antitoxin Systems
Authors: Maggi Stefano , Ferrari Alberto , Yabre Korotoum , Bonini Aleksandra Anna , Rivetti Claudio , Folli Claudia
Published: October 2021

A large number of bacterial toxin–antitoxin (TA) systems have been identified so far and different experimental approaches have been explored to investigate their activity and regulation both in vivo and in vitro. Nonetheless, a common feature of these methods is represented by the difficulty in cell transformation, culturing, and stability of the transformants, due to the expression of highly toxic proteins. Recently, in dealing with the type I Lpt/RNAII and the type II YafQ/DinJ TA systems, we encountered several of these problems that urged us to optimize methodological strategies to study the phenotype of recombinant Escherichia coli host cells. In particular, we have found conditions to tightly repress toxin expression by combining the pET expression system with the E. coli C41(DE3) pLysS strain. To monitor the RNase activity of the YafQ toxin, we developed a fluorescence approach based on Thioflavin-T which fluoresces brightly when complexed with bacterial RNA. Fluorescence microscopy was also applied to reveal loss of membrane integrity associated with the activity of the type I toxin Lpt, by using DAPI and ethidium bromide to selectively stain cells with impaired membrane permeability. We further found that atomic force microscopy can readily be employed to characterize toxin-induced membrane damages.
High Transient-Thermal-Shock Resistant Nanochannel Tungsten Films
Authors: Cheng Tao , Qin Wenjing , Lian Youyun , Liu Xiang , Tang Jun , Cai Guangxu , Zhang Shijian , Le Xiaoyun , Jiang Changzhong , Ren Feng
Published: October 2021

Developing high-performance tungsten plasma-facing materials for fusion reactors is an urgent task. In this paper, novel nanochannel structural W films prepared by magnetron sputtering deposition were irradiated using a high-power pulsed electron beam or ion beam to study their edge-localized modes, such as transient thermal shock resistance. Under electron beam irradiation, a 1 μm thick nanochannel W film with 150 watt power showed a higher absorbed power density related cracking threshold (0.28–0.43 GW/m2) than the commercial bulk W (0.16–0.28 GW/m2) at room temperature. With ion beam irradiation with an energy density of 1 J/cm2 for different pulses, the bulk W displayed many large cracks with the increase of pulse number, while only micro-crack networks with a width of tens of nanometers were found in the nanochannel W film. For the mechanism of the high resistance of nanochannel W films to transient thermal shock, a residual stress analysis was made by Grazing-incidence X-ray diffraction (GIXRD), and the results showed that the irradiated nanochannel W films had a much lower stress than that of the irradiated bulk W, which indicates that the nanochannel structure can release more stress, due to its special nanochannel structure and ability for the annihilation of irradiation induced defects.
Characterization, Antimicrobial and Anticancer Properties of Palladium Nanoparticles Biosynthesized Optimally Using Saudi Propolis
Authors: Al-Fakeh Maged S. , Osman Samir Osman Mohammed , Gassoumi Malek , Rabhi Mokded , Omer Mohamed
Published: October 2021

Due to their unique physicochemical characteristics, palladium nanoparticles (Pd-NPs) have shown tremendous promise in biological applications. The biosynthesis of Pd-NPs employing Saudi propolis has been designed to be environmental, fast, controlled, and cost-effective. The formation and stability of biosynthesized Pd-NPs by Saudi propolis extract were proved by ultraviolet–visible spectrophotometry (UV-Vis), Fourier-transform infrared spectroscopy (FT-IR), and Zeta potential analysis. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray diffraction (XRD) findings show that the average particle size of Pd-NPs is between 3.14 and 4.62 nm, which is in quantum scale. The Saudi propolis enhanced the antimicrobial activity against B. subtilis, S. aureus, E. coli, K. pneumoniae, and C. albicans. Pd-NPs show effective anticancer activity against ductal carcinoma (MCF-7) with IC50 of 104.79 µg/mL.
Mechanisms of Chitosan Nanoparticles in the Regulation of Cold Stress Resistance in Banana Plants
Authors: Wang Anbang , Li Jingyang , AL-Huqail Arwa Abdulkreem , AL-Harbi Mohammad S. , Ali Esmat F. , Wang Jiashui , Ding Zheli , Rekaby Saudi A. , Ghoneim Adel M. , Eissa Mamdouh A.
Published: October 2021

Exposure of banana plants, one of the most important tropical and subtropical plants, to low temperatures causes a severe drop in productivity, as they are sensitive to cold and do not have a strong defense system against chilling. Therefore, this study aimed to improve the growth and resistance to cold stress of banana plants using foliar treatments of chitosan nanoparticles (CH-NPs). CH-NPs produced by nanotechnology have been used to enhance tolerance and plant growth under different abiotic stresses, e.g., salinity and drought; however, there is little information available about their effects on banana plants under cold stress. In this study, banana plants were sprayed with four concentrations of CH-NPs—i.e., 0, 100, 200, and 400 mg L−1 of deionized water—and a group that had not been cold stressed or undergone CH-NP treatment was used as control. Banana plants (Musa acuminata var. Baxi) were grown in a growth chamber and exposed to cold stress (5 °C for 72 h). Foliar application of CH-NPs caused significant increases (p < 0.05) in most of the growth parameters and in the nutrient content of the banana plants. Spraying banana plants with CH-NPs (400 mg L−1) increased the fresh and dry weights by 14 and 41%, respectively, compared to the control. A positive correlation was found between the foliar application of CH-NPs, on the one hand, and photosynthesis pigments and antioxidant enzyme activities on the other. Spraying banana plants with CH-NPs decreased malondialdehyde (MDA) and reactive oxygen species (ROS), i.e., hydrogen peroxide (H2O2), hydroxyl radicals (•OH), and superoxide anions (O2•−). CH-NPs (400 mg L−1) decreased MDA, H2O2, •OH, and O2•− by 33, 33, 40, and 48%, respectively, compared to the unsprayed plants. We hypothesize that CH-NPs increase the efficiency of banana plants in the face of cold stress by reducing the accumulation of reactive oxygen species and, in consequence, the degree of oxidative stress. The accumulation of osmoprotectants (soluble carbohydrates, proline, and amino acids) contributed to enhancing the cold stress tolerance in the banana plants. Foliar application of CH-NPs can be used as a sustainable and economically feasible approach to achieving cold stress tolerance.
New Insights into the Reactivity of Detonation Nanodiamonds during the First Stages of Graphitization
Authors: Ducrozet Florent , Girard Hugues A. , Leroy Jocelyne , Larquet Eric , Florea Ileana , Brun Emilie , Sicard-Roselli Cécile , Arnault Jean-Charles
Published: October 2021

The present study aims to compare the early stages of graphitization of the same DND source for two annealing atmospheres (primary vacuum, argon at atmospheric pressure) in an identical set-up. DND samples are finely characterized by a combination of complementary techniques (FTIR, Raman, XPS, HR-TEM) to highlight the induced modifications for temperature up to 1100 °C. The annealing atmosphere has a significant impact on the graphitization kinetics with a higher fraction of sp2-C formed under vacuum compared to argon for the same temperature. Whatever the annealing atmosphere, carbon hydrogen bonds are created at the DND surface during annealing according to FTIR. A “nano effect”, specific to the <10 nm size of DND, exalts the extreme surface chemistry in XPS analysis. According to HR-TEM images, the graphitization is limited to the first outer shell even for DND annealed at 1100 °C under vacuum.
Ice Pack Test Eased Ptosis in a Patient Presenting with a Possible Oculomotor Nerve Schwannoma: A Case Report
Authors: Buechner Susanne , Capone Loredana
Published: October 2021

A 32-year-old man presented with complete third nerve palsy. Brain magnetic resonance imaging revealed a possible sporadic oculomotor nerve schwannoma in the left cavernous sinus. Interestingly, the patient’s ptosis was fluctuating and eased immediately after application of ice over his eyelid. The ice pack test (IPT) is a simple and fast method that can help diagnose myasthenia gravis (MG) in patients with ptosis. Additional diagnostic investigations (antibody assays and repetitive stimulation) excluded associated MG in the patient. Tumor treatment with Gamma Knife radiosurgery was planned. This case highlights the finding that IPT can also be positive in neurogenic (non-myasthenic) ptosis, but its usefulness in other disorders associated with muscle weakness and fatigability remains questionable.
Augmented Renal Clearance, Muscle Catabolism and Urinary Nitrogen Loss: Implications for Nutritional Support in Critically Ill Trauma Patients
Authors: Dreydemy Guilhem , Coussy Alexis , Lannou Alexandre , Petit Laurent , Biais Matthieu , Carrié Cédric
Published: October 2021

The main objective of this pilot study was to determine the association between augmented renal clearance (ARC), urinary nitrogen loss and muscle wasting in critically ill trauma patients. We conducted a retrospective analysis of a local database in 162 critically ill trauma patients without chronic renal dysfunction. Nutritional-related parameters and 24 h urinary biochemical analyses were prospectively collected and averaged over the first ten days after admission. Augmented renal clearance was defined by a mean creatinine clearance (CLCR) > 130 mL/min/1.73 m2. The main outcome was the cumulated nitrogen balance at day 10. The secondary outcome was the variation of muscle psoas cross-sectional area (ΔCSA) calculated in the subgroup of patients who underwent at least two abdominal CT scans during the ICU length of stay. Overall, there was a significant correlation between mean CLCR and mean urinary nitrogen loss (normalized coefficient: 0.47 ± 0.07, p < 0.0001). ARC was associated with a significantly higher urinary nitrogen loss (17 ± 5 vs. 14 ± 4 g/day, p < 0.0001) and a lower nitrogen balance (−6 ± 5 vs. −4 ± 5 g/day, p = 0.0002), without difference regarding the mean protein intake (0.7 ± 0.2 vs. 0.7 ± 0.3 g/kg/day, p = 0.260). In the subgroup of patients who underwent a second abdominal CT scan (N = 47), both ΔCSA and %ΔCSA were higher in ARC patients (−33 [−41; −25] vs. −15 [−29; −5] mm2/day, p = 0.010 and −3 [−3; −2] vs. −1 [−3; −1] %/day, p = 0.008). Critically ill trauma patients with ARC are thus characterized by a lower nitrogen balance and increased muscle loss over the 10 first days after ICU admission. The interest of an increased protein intake (>1.5 g/kg/day) in such patients remains a matter of controversy and must be confirmed by further randomized trials.
The Effects of Sodium Phosphate Supplementation on the Cardiorespiratory System and Gross Efficiency during Exercise under Hypoxia in Male Cyclists: A Randomized, Placebo-Controlled, Cross-Over Study
Authors: Płoszczyca Kamila , Gajda Robert , Czuba Miłosz
Published: October 2021

The main aim of this study was to evaluate the effects of six days of tri-sodium phosphate (SP) supplementation on the cardiorespiratory system and gross efficiency (GE) during exercise under hypoxia in cyclists. Twenty trained male cyclists received SP (50 mg·kg−1 of fat-free mass/day) or placebo for six days in a randomized, cross-over study, with a three-week washout period between supplementation phases. Before and after each supplementation phase, the subjects performed an incremental exercise test to exhaustion under normobaric hypoxia (FiO2 = 16%, ~2500 m). It was observed that short-term SP supplementation led to a decrease in heart rate, an increase in stroke volume, and an improvement in oxygen pulse (VO2/HR) during low and moderate-intensity exercise under hypoxia. These changes were accompanied by an increase in the serum inorganic phosphate level by 8.7% (p < 0.05). No significant changes were observed in serum calcium levels. GE at a given workload did not change significantly after SP supplementation. These results indicated that SP promotes improvements in the efficiency of the cardiorespiratory system during exercise in a hypoxic environment. Thus, SP supplementation may be beneficial for endurance exercise in hypoxia.
Validation of the Evidence-Based Practice Competence Questionnaire for Nursing Students: A Cross-Sectional Study in Greece
Authors: Patelarou Athina , Schetaki Stefania , Giakoumidakis Konstantinos , Lialiou Paschalina , Patelarou Evridiki
Published: October 2021

(1) Background: evidence-based nursing has been widely adopted by healthcare facilitators, and it is predicated on the connection between research evidence and clinical practice. The knowledge and implementation of evidence-based practice (EBP) depend on a variety of long-established barriers and attitudes. The purpose of this study was to translate and validate the Greek version of the evidence-based practice competence questionnaire (EBP-COQ) and test it on a sample of Greek undergraduate nursing students. (2) Methods: a cross-sectional analysis was conducted on data obtained between November 2018 and January 2019. A convenience sample of 320 Greek undergraduate nursing students participated in a survey to examine the psychometric properties of the tool. The reliability and validity of the tool was examined. Cronbach’s coefficient alpha was used to determine the scale’s internal consistency reliability. (3) Results: the EBP-COQ was translated and validated. The estimated Cronbach’s alpha was higher than 0.70 for all scales. Of the participants, 22.5% were men and 77.5% were women, while 31% of them were in their final year of nursing education. A total of 61% of the students stated that they had not participated in an EBP seminar in the past. High Spearman’s correlation coefficients were found for “Knowledge in EBP” with “Personal attitude towards EBP” (rs = 0.329, p < 0.001). (4) Conclusions: the Greek version of the EBP-COQ is a valid instrument that can be used in the Greek population. It provides information about attitude, knowledge and skills in the EBP approach.
Recent Advances in the Synthesis of Piperazines: Focus on C–H Functionalization
Authors: Durand Carolina , Szostak Michal
Published: October 2021

Piperazine ranks as the third most common nitrogen heterocycle in drug discovery, and it is the key component of several blockbuster drugs, such as Imatinib (also marketed as Gleevec) or Sildenafil, sold as Viagra. Despite its wide use in medicinal chemistry, the structural diversity of piperazines is limited, with about 80% of piperazine-containing drugs containing substituents only at the nitrogen positions. Recently, major advances have been made in the C–H functionalization of the carbon atoms of the piperazine ring. Herein, we present an overview of the recent synthetic methods to afford functionalized piperazines with a focus on C–H functionalization.
Comparison of Autistic Individuals Who Engage in Self-Injurious Behavior, Aggression, and Both Behaviors
Authors: Edelson Stephen M.
Published: October 2021

Background. Two of the most challenging behaviors exhibited by individuals on the autism spectrum are self-injurious behavior (SIB) and aggression. The aim of this study was to identify co-occurring symptoms, behaviors, and medical comorbidities that may provide insight into understanding and treating these behaviors. Method. A large-scale online survey was used to collect data on symptoms, behaviors, and medically related comorbidities commonly reported in individuals with autism spectrum disorders (ASD). Based on responses from 2327 participants, individuals with ASD were divided into four categories: individuals who engaged in SIB only, individuals who engaged in aggression only, individuals who engaged in both behaviors, and individuals who engaged in neither behavior. Results. There were several characteristics and comorbidities associated with those who engaged in SIB only and in aggression only, in addition to those who engaged in both behaviors. Conclusion. The findings in this study provide evidence to support at least two underlying causes of these behaviors (insensitivity to pain and reactions to food) as well as implications for treating them. Furthermore, several behaviors often observed during early childhood may be considered early predictors of these challenging behaviors.
Brain Perivascular Macrophages Do Not Mediate Interleukin-1-Induced Sickness Behavior in Rats
Authors: Chaskiel Léa , Dantzer Robert , Konsman Jan
Published: October 2021

Sickness behavior, characterized by on overall reduction in behavioral activity, is commonly observed after bacterial infection. Sickness behavior can also be induced by the peripheral administration of Gram-negative bacterial lipopolysaccharide (LPS) or interleukin-1beta (IL-1β), a pro-inflammatory cytokine released by LPS-activated macrophages. In addition to the microglia, the brain contains perivascular macrophages, which express the IL-1 type 1 receptor (IL-1R1). In the present study, we assessed the role of brain perivascular macrophages in mediating IL-1β-induced sickness behavior in rats. To do so, we used intracerebroventricular (icv) administration of an IL-1β-saporin conjugate, known to eliminate IL-R1-expressing brain cells, prior to systemic or central IL-1β injection. Icv IL-1β-saporin administration resulted in a reduction in brain perivascular macrophages, without altering subsequent icv or ip IL-1β-induced reductions in food intake, locomotor activity, and social interactions. In conclusion, the present work shows that icv IL-1β-saporin administration is an efficient way to target brain perivascular macrophages, and to determine whether these cells are involved in IL-1β-induced sickness behavior.
Bronchodilator Delivery via High-Flow Nasal Cannula: A Randomized Controlled Trial to Compare the Effects of Gas Flows
Authors: Li Jie , Chen Yibing , Ehrmann Stephan , Wu Jie , Xie Lixin , Fink James B
Published: October 2021

(1) Background: Aerosol delivery via high-flow nasal cannula (HFNC) has attracted increasing clinical interest. In vitro studies report that the ratio of HFNC gas flow to patient inspiratory flow (GF:IF) is a key factor in the efficiency of trans-nasal aerosol delivery. (2) Methods: In a randomized controlled trial, patients with a history of COPD or asthma and documented positive responses to inhaled bronchodilators in an outpatient pulmonary function laboratory were recruited. Subjects were randomized to receive inhalation at gas flow ratio settings of: GF:IF = 0.5, GF:IF = 1.0, or GF = 50 L/min. Subjects were assigned to inhale saline (control) followed by salbutamol via HFNC with cumulative doses of 0.5 mg, 1.5 mg, 3.5 mg, and 7.5 mg. Spirometry was performed at baseline and 10–12 min after each inhalation. (3) Results: 75 subjects (49 asthma and 26 COPD) demonstrating bronchodilator response were enrolled. Per the robust ATS/ERS criteria no difference was observed between flows, however using the criteria of post-bronchodilator forced expiratory volume in the first second (FEV1) reaching the screening post-bronchodilator FEV1 with salbutamol, a higher percentage of subjects receiving GF:IF = 0.5 met the criteria at a cumulative dose of 1.5 mg than those receiving GF:IF = 1.0, and GF = 50 L/min (64% vs. 29% vs. 27%, respectively, p = 0.011). Similarly at 3.5 mg (88% vs. 54% vs. 46%, respectively, p = 0.005). The effective dose at GF:IF = 0.5 was 1.5 mg while for GF = 50 L/min it was 3.5 mg. (4) Conclusions: During salbutamol delivery via HFNC, cumulative doses of 1.5 mg to 3.5 mg resulted in effective bronchodilation. Applying the robust ATS/ERS criteria no difference was observed between the flows, however using the more sensitive criteria of subjects reaching post screening FEV1 to salbutamol via HFNC, a higher number of subjects responded to the doses of 0.5 mg and 1.5 mg when HFNC gas flow was set at 50% of patient peak inspiratory flow.
Composite Drug Delivery System Based on Amorphous Calcium Phosphate–Chitosan: An Efficient Antimicrobial Platform for Extended Release of Tetracycline
Authors: Visan Anita Ioana , Ristoscu Carmen , Popescu-Pelin Gianina , Sopronyi Mihai , Matei Consuela Elena , Socol Gabriel , Chifiriuc Mariana Carmen , Bleotu Coralia , Grossin David , Brouillet Fabien , Grill Sylvain Le , Bertrand Ghislaine , Zgura Irina , Cristescu Rodica , Mihailescu Ion N.
Published: October 2021

One major warning emerging during the first worldwide combat against healthcare-associated infections concerns the key role of the surface in the storage and transfer of the virus. Our study is based on the laser coating of surfaces with an inorganic/organic composite mixture of amorphous calcium phosphate–chitosan–tetracycline that is able to fight against infectious agents, but also capable of preserving its activity for a prolonged time, up to several days. The extended release in simulated fluids of the composite mixture containing the drug (tetracycline) was demonstrated by mass loss and UV–VIS investigations. The drug release profile from our composite coatings proceeds via two stages: an initial burst release (during the first hours), followed by a slower evolution active for the next 72 h, and probably more. Optimized coatings strongly inhibit the growth of tested bacteria (Enterococcus faecalis and Escherichia coli), while the drug incorporation has no impact on the in vitro composite’s cytotoxicity, the coatings proving an excellent biocompatibility sustaining the normal development of MG63 bone-like cells. One may, therefore, consider that the proposed coatings’ composition can open the prospective of a new generation of antimicrobial coatings for implants, but also for nosocomial and other large area contamination prevention.
Plant-Mediated Zinc Oxide Nanoparticles: Advances in the New Millennium towards Understanding Their Therapeutic Role in Biomedical Applications
Authors: Murali Mahadevamurthy , Kalegowda Nataraj , Gowtham Hittanahallikoppal G. , Ansari Mohammad Azam , Alomary Mohammad N. , Alghamdi Saad , Shilpa Natarajamurthy , Singh Sudarshana B. , Thriveni M. C. , Aiyaz Mohammed , Angaswamy Nataraju , Lakshmidevi Nanjaiah , Adil Syed F. , Hatshan Mohammad R. , Amruthesh Kestur Nagaraj
Published: October 2021

Zinc oxide nanoparticles have become one of the most popular metal oxide nanoparticles and recently emerged as a promising potential candidate in the fields of optical, electrical, food packaging, and biomedical applications due to their biocompatibility, low toxicity, and low cost. They have a role in cell apoptosis, as they trigger excessive reactive oxygen species (ROS) formation and release zinc ions (Zn2+) that induce cell death. The zinc oxide nanoparticles synthesized using the plant extracts appear to be simple, safer, sustainable, and more environmentally friendly compared to the physical and chemical routes. These biosynthesized nanoparticles possess strong biological activities and are in use for various biological applications in several industries. Initially, the present review discusses the synthesis and recent advances of zinc oxide nanoparticles from plant sources (such as leaves, stems, bark, roots, rhizomes, fruits, flowers, and seeds) and their biomedical applications (such as antimicrobial, antioxidant, antidiabetic, anticancer, anti-inflammatory, photocatalytic, wound healing, and drug delivery), followed by their mechanisms of action involved in detail. This review also covers the drug delivery application of plant-mediated zinc oxide nanoparticles, focusing on the drug-loading mechanism, stimuli-responsive controlled release, and therapeutic effect. Finally, the future direction of these synthesized zinc oxide nanoparticles’ research and applications are discussed.
Student Pharmacists during the Pandemic: Development of a COVID-19 Knowledge, Attitudes, and Practices (COVKAP) Survey
Authors: Cernasev Alina , Desai Meghana , Jonkman Lauren J. , Connor Sharon E. , Ware Nicholas , Sekar M. Chandra , Schommer Jon C.
Published: October 2021

Background: The COVID-19 pandemic has caused innumerable changes to all aspects of human life and behavior, including academic life. This study describes the development of a COVID-19 Knowledge, Attitudes, and Practices (COVKAP) Survey among U.S. student pharmacists. The survey was administered at Doctor of Pharmacy programs in three states—Tennessee, Ohio, and Pennsylvania. Methods: The COVKAP survey—an online cross-sectional survey—was distributed to U.S. student pharmacists enrolled in three different colleges of pharmacy in three states during the fall semester of 2020. The survey was developed using literature review and Dillman’s recommendations for survey design. The COVKAP survey consisted of 23 closed and Likert-scale questions, and three open-ended questions. The research team conducted descriptive and inductive thematic analyses on the quantitative and qualitative data, respectively using SPSS (v27) and Dedoose® software. Results: A total of 421 responses were received. Respondents were predominantly female (72%) and White (79%). The average age of respondents was 23.4 years. The qualitative analysis revealed three themes: (1) Wellbeing and mental health struggles; (2) Being part of the decision-making process; (3) Necessity of adequate protection measures. Conclusions: Preliminary study findings indicate that student pharmacists’ concerns and the challenges that they face in their academic pursuits are largely similar across the three states in this study and inform about the importance of recognizing and mitigating the impact of widespread disruption in education. This disruption provides an opportunity for pharmacy academia to examine practices and methods that can be improved upon to help students become successful practitioners.
Supporting COVID-19 Vaccine Rollout before Charter Class Arrives: The University of California, Irvine Experience
Authors: Chan Alexandre , Joe Melanie D. , Hirsch Jan D.
Published: October 2021

Despite numerous challenges in relation to being a recently established school, the University of California, Irvine (UCI) School of Pharmacy and Pharmaceutical Sciences (SPPS), similar to many schools of pharmacy in the United States, was highly committed to supporting the rollout of COVID-19 vaccines. UCI SPPS and our affiliated UCI Medical Center (UCIMC) Pharmacy Department partnered to spearhead the pharmacy element of a large-scale COVID-19 vaccination clinic on campus for both employees and the community. Three key initiatives were established in order to overcome the obstacles we faced in the large-scale roll out of COVID-19 vaccines: (1) forging new collaborations within the pharmacy team, (2) leveraging interprofessional education and practice, and (3) raising awareness of the pharmacists’ role. Our response to the COVID-19 vaccines at UCI was a tangible, visible model that demonstrated that, while we continue to embrace our role in team-based, patient-centered care, it is also important for us to step up and lead the profession. Additionally, this vaccine rollout experience is a teachable moment for our communities and our health professional partners as we continue to march forward as one voice to serve the American public.
Nonstandard Null Lagrangians and Gauge Functions for Newtonian Law of Inertia
Authors: Musielak Zdzislaw E.
Published: October 2021

New null Lagrangians and gauge functions are derived and they are called nonstandard because their forms are different than those previously found. The invariance of the action is used to make the Lagrangians and gauge functions exact. The first exact nonstandard null Lagrangian and its gauge function for the law of inertia are obtained, and their physical implications are discussed.
Investigation of Quinoa Seeds Fractions and Their Application in Wheat Bread Production
Authors: Coţovanu Ionica , Ungureanu-Iuga Mădălina , Mironeasa Silvia
Published: October 2021

The present study aimed to investigate the influence of quinoa fractions (QF) on the chemical components of wheat flour (WF), dough rheological properties, and baking performance of wheat bread. The microstructure and molecular conformations of QF fractions were dependent to the particle size. The protein, lipids, and ash contents of composite flours increased with the increase of QF addition level, while particle size (PS) decreased these parameters as follows: Medium ˃ Small ˃ Large, the values being higher compared with the control (WF). QF addition raised dough tenacity from 86.33 to 117.00 mm H2O, except for the small fraction, and decreased the extensibility from 94.00 to 26.00 mm, while PS determined an irregular trend. The highest QF addition levels and PS led to the highest dough viscoelastic moduli (55,420 Pa for QL_20, 65245 Pa for QM_20 and 48305 Pa for QS_20, respectively). Gradual increase of QF determined dough hardness increase and adhesiveness decrease. Bread firmness, springiness, and gumminess rises were proportional to the addition level. The volume, elasticity, and porosity of bread decreased with QF addition. Flour and bread crust and crumb color parameters were also influenced by QF addition with different PS.
In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract
Authors: Mare Anca Delia , Ciurea Cristina Nicoleta , Man Adrian , Mareș Mihai , Toma Felicia , Berța Lavinia , Tanase Corneliu
Published: October 2021

Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on different Candida spp., and their influence on virulence factors of C. albicans (germ tube production, gene expression for ALS3, SAP2, HSP70). Both the AgNP BBEs presented different minimum inhibitory concentrations for all the studied Candida spp., but biofilm production was inhibited only for C. albicans and C. guilliermondii. The growth rates of all the studied Candida spp. were inhibited in the presence of both AgNP BBEs, except for C. auris. Synergistic activity was observed for C. parapsilosis and C. guilliermondii, for different combinations of fluconazole with both the AgNP BBEs. The germ tube production of C. albicans was slightly inhibited by the AgNP BBEs. Only AgNP Acetate BBE was able to down-regulate the expression of SAP2. Overall, we can conclude that, even if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting alternative to classic antifungal treatments.
Thonningia sanguinea Extract: Antioxidant and Cytotoxic Activities Supported by Chemical Composition and Molecular Docking Simulations
Authors: Abdelhameed Reda F. A. , Elhady Sameh S. , Sirwi Alaa , Samir Hanan , Ibrahim Elsayed A. , Thomford Ama Kyeraa , El Gindy Alaa , Hadad Ghada M. , Badr Jihan M. , Nafie Mohamed S.
Published: October 2021

The current study was designed to investigate the antioxidant and cytotoxic activities of Thonningia sanguinea whole-plant extract. The total phenolic content was determined using Folin–Ciocalteu reagent and found to be 980.1 mg/g, calculated as gallic acid equivalents. The antioxidant capacity was estimated for the crude extract and the phenolic portion of T. sanguinea, whereupon both revealed a dose-dependent scavenging rate of DPPH• with EC50 values of 36.33 and 11.14 µg/mL, respectively. Chemical profiling of the plant extract was achieved by LC-ESI-TOF-MS/MS analysis, where 17 compounds were assigned, including ten compounds detected in the negative mode and seven detected in the positive mode. The phenolic portion exhibited promising cytotoxic activity against MCF-7 and HepG2 cells, with IC50 values of 16.67 and 13.51 μg/mL, respectively. Phenolic extract treatment caused apoptosis in MCF-7 cells, with total apoptotic cell death 18.45-fold higher compared to untreated controls, arresting the cell cycle at G2/M by increasing the G2 population by 39.7%, compared to 19.35% for the control. The apoptotic investigation was further validated by the upregulation of proapoptotic genes of P53, Bax, and caspases-3,8 9, and the downregulation of Bcl-2 as the anti-apoptotic gene. Bcl-2 inhibition was also virtualized by good binding interactions through a molecular docking study. Taken together, phenolic extract exhibited promising cytotoxic activity in MCF-7 cells through apoptosis induction and antioxidant activation, so further fractionation studies are recommended for the phenolic extract for specifying the most active compound to be developed as a novel anti-cancer agent.
Characteristics of PEO Incorporated with CaTiO3 Nanoparticles: Structural and Optical Properties
Authors: Aziz Shujahadeen B. , Nofal Muaffaq M. , Brza Mohamad A. , Hussein Sarkawt A. , Mahmoud Khaled H. , El-Bahy Zeinhom M. , Dannoun Elham M. A. , Kareem Wrya O. , Hussein Ahang M.
Published: October 2021

In this research, direct band gap polymer composites with amorphous phase, which are imperative for optoelectronic devices applications were synthesized. The solution cast technique was used to produce polyethylene oxide (PEO)/calcium titanate (CaTiO3) nanocomposite (NC) films. The X-ray diffraction (XRD) confirms the growth of amorphous nature within PEO with CaTiO3 addition. The optical band gaps of pure PEO and PEO/CaTiO3 NC films were calculated using analysis of ultraviolet–visible (UV-Vis) spectra. The change in absorption edge toward lower photon energy is evidence of polymer modification. The dispersion behavior of the refractive index of PEO was manipulated to a higher wavelength upon doping with CaTiO3. Upon adding CaTiO3 to the pure PEO polymer, the dielectric constant and refractive index were considerably modified. The band gap shifts from 4.90 eV to 4.19 eV for the PEO incorporated with an optimum portion of 8 wt. % of CaTiO3. The types of the electronic transition in composite samples were specified, based on the Taucs model and the optical dielectric loss. The alteration of UV/Vis absorption spectra of the NC film was considered a suitable candidate to be applied in nanotechnology-based devices. The spherulites ascribed to the crystalline phase were distinguished through the optical microscopy (OM) study.
Surface Treatment of Composites with Bismaleimide Resin-Based Wet Peel Ply for Enhanced Adhesive Bonding Performance
Authors: Li Hongfeng , Zhao Liwei , Qiao Yingjie , Bai Xuefeng , Wang Dezhi , Qu Chunyan , Liu Changwei , Wang Yongqiang
Published: October 2021

Surface treatment is typically required to improve the bonding performance of carbon-fiber-reinforced composites. Herein, a wet peel ply was prepared using bismaleimide (BMI) resins as a matrix resin. The temperature–heating rate extrapolation method and rheological method were employed to study the reaction characteristics and viscosity-temperature characteristics of the matrix in the BMI wet peel ply. The curing temperatures of the BMI wet peel ply and the BMI prepreg were the same (200 °C), making this wet peel ply suitable for co-curing with the BMI prepreg. After treatment with the wet peel ply, the bonding strength of the BMI composite joint showed a mean shear strength of 35.5 MPa, which was 1.72% higher than that of the sanded composite and 17.5% higher than that of the composite treated with the dry peel ply. In addition, the BMI composite treated with the BMI wet peel ply exhibited good bonding stability with a coefficient of variation of 3.9. After damp-heat aging for 1440 h, the retention rate of shear strength at room-temperature was 82.3%. The relatively loosely woven carrier in the BMI wet peel ply increased the surface roughness of the composite, thus improving the bonding strength.
Crosslinked Fluorinated Poly(arylene ether)s with POSS: Synthesis and Conversion to High-Performance Polymers
Authors: He Yashu , Wang Jingting , Sirotin Igor S. , Kireev Vyacheslav V. , Mu Jianxin
Published: October 2021

This study reports on a series of crosslinked poly(arylene ether)s with POSS in the main chain. The fluorinated and terminated poly(arylene ether)s were first synthesized by the nucleophilic reaction of diphenol POSS and decafluorodiphenyl monomers, including decafluorobiphenyl, decaflurobenzophenone, and decafluorodiphenyl sulfone. They were then reacted with 3-hydroxyphenyl acetylene to produce phenylacetylene-terminated poly(arylene ether)s. The polymers were of excellent processability. When heated to a high temperature, the polymers converted into a crosslinked network, exhibiting a low range of dielectric constant from 2.17 to 2.58 at 1 HMz, strong resistance against chemical solutions, low dielectric losses, and good thermal and hydrophobic properties.
Prediction of the Ultimate Strength of Notched and Unnotched IM7/977-3 Laminated Composites Using a Micromechanics Approach
Authors: Masud Manzar , Kharusi Moosa S. M. Al , Ali Muhammad Umair , Mubashar Aamir , Hussain Shaik Javeed , Tariq Adnan , Rehman Gulfam Ul , Akhtar Mahmood Hassan , Javeed Shama
Published: October 2021

This paper proposes a multi-scale analysis technique based on the micromechanics of failure (MMF) to predict and investigate the damage progression and ultimate strength at failure of laminated composites. A lamina’s representative volume element (RVE) is developed to predict and calculate constituent stresses. Damages that occurred in the constituents are calculated using separate failure criteria for both fiber and matrix. Subsequently, the volume-based damage homogenization technique is utilized to prevent the localization of damage throughout the total matrix zone. The proposed multiscale analysis procedure is then used to investigate the notched and unnotched behavior of three multi-directional composite layups, [30, 60, 90, −60, 30]2S, [0, 45, 90, −45]2S, and [60, 0, −60]3S, subjected to static tension and compression loading. The specimen is fabricated from unidirectionally reinforced composite (IM7/977-3). The prediction of ultimate strength at failure and equivalent stiffness are then benchmarked against the experimental test data. The comparative analysis with various failure models is also carried out to validate the proposed model. MMF demonstrated the capability to correctly predict the ultimate strength at failure for a range of multidirectional composites laminates under tensile and compressive load. The numerically predicted findings revealed a good agreement with the experimental test data. Out of the three investigated composite layups, the simulated results for the quasi-isotropic [0, 45, 90, −45]2S layup agreed extremely well with the experimental results with all the percentage errors within 10% of the measured failure loads.