bluuebunny/crossref_metadata_2025 huggingface dataset:
Overview of Virulence and Antibiotic Resistance in Campylobacter spp. Livestock Isolates
Authors: Bunduruș Iulia Adelina , Balta Igori , Ștef Lavinia , Ahmadi Mirela , Peț Ioan , McCleery David , Corcionivoschi Nicolae
Published: February 2023

Campylobacter remains the most prevalent foodborne pathogen bacterium responsible for causing gastroenteritis worldwide. Specifically, this pathogen colonises a ubiquitous range of environments, from poultry, companion pets and livestock animals to humans. The bacterium is uniquely adaptable to various niches, leading to complicated gastroenteritis and, in some cases, difficult to treat due to elevated resistance to certain antibiotics. This increased resistance is currently detected via genomic, clinical or epidemiological studies, with the results highlighting worrying multi-drug resistant (MDR) profiles in many food and clinical isolates. The Campylobacter genome encodes a rich inventory of virulence factors offering the bacterium the ability to influence host immune defences, survive antimicrobials, form biofilms and ultimately boost its infection-inducing potential. The virulence traits responsible for inducing clinical signs are not sufficiently defined because several populations have ample virulence genes with physiological functions that reflect their pathogenicity differences as well as a complement of antimicrobial resistance (AMR) systems. Therefore, exhaustive knowledge of the virulence factors associated with Campylobacter is crucial for collecting molecular insights into the infectivity processes, which could pave the way for new therapeutical targets to combat and control the infection and mitigate the spread of MDR bacteria. This review provides an overview of the spread and prevalence of genetic determinants associated with virulence and antibiotic resistance from studies performed on livestock animals. In addition, we have investigated the relevant coincidental associations between the prevalence of the genes responsible for pathogenic virulence, horizontal gene transfer (HGT) and transmissibility of highly pathogenic Campylobacter strains.
Oxidative Stress as a Reliable Biomarker of Carotid Plaque Instability: A Pilot Study
Authors: Svoboda Norbert , Kočí Karolina , Seidlová Anna , Mandys Václav , Suttnar Jiří , Hlaváčková Alžběta , Kučerka Ondřej , Netuka David , Malý Martin
Published: February 2023

Background: Predicting stroke risk in patients with carotid artery stenosis (CS) remains challenging. Circulating biomarkers seem to provide improvements with respect to risk stratification. Methods: Study patients who underwent carotid endarterectomy were categorized into four groups according to symptomatology and compared as follows: symptomatic with asymptomatic patients; and asymptomatic patients including amaurosis fugax (AF) (asymptomatic + AF group) with patients with a transient ischemic attack (TIA) or brain stroke (BS) (hemispheric brain stroke group). Carotid specimens were histologically analyzed and classified based on the American Heart Classification (AHA) standard. As a marker of OS, the plasma levels of malondialdehyde (MDA) were measured. Comparisons of MDA plasma levels between groups were analyzed. Results: In total, 35 patients were included in the study. There were 22 (63%) patients in the asymptomatic group and 13 (37%) in the symptomatic group. Atheromatous plaque (p = 0.03) and old hemorrhage (p = 0.05), fibrous plaque (p = 0.04), myxoid changes (p = 0.02), plaques without hemorrhage (p = 0.04), significant neovascularization (p = 0.04) and AHA classification (p = 0.006) had significant correlations with clinical presentation. There were 26 (74%) patients in the asymptomatic group and 9 (26%) in the hemispheric brain stroke group. Atheromatous plaque (p = 0.02), old hemorrhage (p = 0.05) and plaques without neovascularization (p = 0.02), fibrous plaque (p = 0.03), plaques without hemorrhage (p = 0.02) and AHA classification (p = 0.01) had significant correlations with clinical presentation. There was no significant difference between symptomatic and asymptomatic groups with respect to MDA plasma levels (p = 0.232). A significant difference was observed when MDA plasma levels were compared to asymptomatic + AF and the hemispheric stroke group (p = 0.002). Conclusions: MDA plasma level correlates with the risk of hemispheric stroke (TIA or BS) and is a reliable marker of plaque vulnerability in carotid artery stenosis.
Effect of Dietary Phenolic Compounds on Incidence of Type 2 Diabetes in the “Seguimiento Universidad de Navarra” (SUN) Cohort
Authors: Vázquez-Ruiz Zenaida , Martínez-González Miguel Ángel , Vitelli-Storelli Facundo , Bes-Rastrollo Maira , Basterra-Gortari Francisco Javier , Toledo Estefanía
Published: February 2023

The global incidence of type 2 diabetes (T2D) has been steadily increasing in recent decades. The Mediterranean dietary pattern has shown a preventive effect on the risk of T2D. Evaluating the association between bioactive compounds such as phenolic compounds (PC) in a Mediterranean cohort could help to better understand the mechanisms implicated in this protection. We evaluated the association between dietary intake of PC and the risk of T2D in a relatively young cohort of 17,821 Spanish participants initially free of T2D, through the University of Navarra Follow-up Project (“Seguimiento Universidad de Navarra” or SUN cohort) after 10 years of median follow-up using time-dependent Cox models. Intake of PC was estimated at baseline and repeatedly at 10-year follow-up using a 136-item validated food frequency and the Phenol-Explorer database. The incidence of T2D was identified by a biennial follow-up, and only medically confirmed cases were included. During 224,751 person-years of follow-up, 186 cases of T2D were confirmed. A suboptimal intake of stilbenes was independently associated with a higher risk of T2D in subjects over 50 years (HR: 1.75, 95% CI: 1.06–2.90, p value < 0.05) after adjusting for potential confounders. Our results suggest that a moderate-high intake of stilbenes can decrease the risk of developing T2D in subjects over 50 years in our cohort.
One-Body Capillary Plasma Source for Plasma Accelerator Research at e-LABs
Authors: Lee Sihyeon , Kwon Seong-hoon , Nam Inhyuk , Cho Myung-Hoon , Jang Dogeun , Suk Hyyong , Kim Minseok
Published: February 2023

We report on the development of a compact, gas-filled capillary plasma source for plasma accelerator applications. The one-body sapphire capillary was created through a diamond machining technique, which enabled a straightforward and efficient manufacturing process. The effectiveness of the capillary as a plasma acceleration source was investigated through laser wakefield acceleration experiments with a helium-filled gas cell, resulting in the production of stable electron beams of 200 MeV. Discharge capillary plasma was generated using a pulsed, high-voltage system for potential use as an active plasma lens. A peak current of 140 A, corresponding to a focusing gradient of 97 T/m, was observed at a voltage of 10 kV. These results demonstrate the potential utility of the developed capillary plasma source in plasma accelerator research using electron beams from a photocathode gun.
Copper Bioavailability and Leaching in Conventional and Organic Viticulture under Environmental Stress
Authors: Jez Erika , Pellegrini Elisa , Contin Marco
Published: February 2023

The continuous use of Cu-based fungicides in viticulture has caused Cu accumulation in soils, which represents a major environmental and toxicological concern. The purpose of this study was to verify whether the organic management would be more resilient to temperature and moisture stresses in comparison to conventional practices. Two organic and two conventional vineyard soils, contrasting in pH, were exposed for six months to temperature stress (29 °C), moisture stress (10% water holding capacity—WHC), and combined stresses (29 °C—10% WHC). Main soil properties, Cu speciation, bioavailability, and leaching were monitored before and after 6 months’ incubation. Results confirm that the increase of temperature caused a decrease in both total organic carbon (TOC) and dissolved organic carbon (DOC) (up to 19% and 49%, respectively), a decrease in available ammonium, and an increase in nitrate. Moisture stress tends to mitigate some of these changes. Despite that, changes of Cu bioavailability and leaching were limited and rarely significant. Moreover, no regular trends between conventional and organic management could be observed. Changes in soil pH and total N (TN) appeared as the most influencing properties to regulate Cu behavior in vineyard soils. Calcareous soils were more resistant to environmental stresses compared to acid soils, regardless of the type of management (conventional or organic).
Apricot Tree Nutrient Uptake, Fruit Quality and Phytochemical Attributes, and Soil Fertility under Organic and Integrated Management
Authors: Roussos Peter Anargyrou , Karabi Anastasia , Anastasiou Loukas , Assimakopoulou Anna , Gasparatos Dionisios
Published: February 2023

Organic cultivation management has generated a great deal of interest during the last decades. As there are numerous conflicting results in the literature on the superiority of organic cultivation over an integrated one, a trial occurred using two apricot cultivars, i.e., ‘Bebecou’ and ‘Diamantopoulou’, under integrated and organic cultivation (three orchards per cultivation system and cultivar). The trial occurred during a single cultivation period under different soil but the same climatic conditions. Fruit physiological (weight, diameters, skin color), organoleptic (pH, titratable acidity, and total soluble solids), and phytochemical (phenolic compounds, carbohydrates, organic acids, antioxidant activity, and carotenoids) attributes were assessed, along with plant nutrition, soil fertility status, and a sensory evaluation panel. The two cultivation practices exhibited similar effects in many of the parameters studied in this trial as well as small differences. Fruit weight was higher under organic management in ‘Bebecou’ and under integrated in ‘Diamantopoulou’, while copper was found at a higher concentration in the leaves of organically managed trees of both cultivars. The cultivar was found to have the greatest effects on the measured variables, while within each cultivation management, the farm also had a great impact, indicating that the outcome of specific cultivation practice is influenced by so many factors that it is almost impossible to come to a general conclusion about which practice is the best. Therefore, more research is needed, focused not only on the differences between organic and integrated cultivation management but primarily on the influence of specific cultivation practices within the same management system, to be able to elucidate, to some extent, the effects of individual factors on the measured variables.
Metal Ballads as Low Pop? An Approach to Sentimentality and Gendered Performances in Popular Hard Rock and Metal Songs
Authors: Nink Theresa , Heesch Florian
Published: February 2023

Ballads are often among the bestselling songs of heavy metal and hard rock bands. Within these genres, ballads represent a way to address emotions such as love that are not part of the primary self-understanding of those genres. Still, “genre ideals and style” often seem to be at odds with the sentimental aesthetics of the ballad and its emotional expression and experience. In this article, we take a close look at the sonic, textual, performative, visual, and emotional-somatic articulation of love and the generation of sentimentality in three selected metal ballads. Even if the term “power ballad,” which is often used in reference to hard rock and metal ballads, refers to the simultaneity of “heaviness” in the sound and the thematization of love in the lyrics, sentimental ballads in the stereotypically more masculine-connotated genres nevertheless create friction and skepticism in their discursive evaluation, as they generate aesthetic discrepancies between concrete songs and genre conventions. Their quantitative popularity contrasts with their qualitative evaluation. Therefore, in a second step, we analyze the reception of the selected ballads, in particular their discursive evaluations in music reviews, in order to point out the ways of argumentation through which frictions are established. As a result, we show that evaluations are related to how love is addressed in the songs and to the extent of proximity of the ballads to genre rules.
Adverse Health Effects (Bronchitis Cases) Due to Particulate Matter Exposure: A Twenty-Year Scenario Analysis for the Greater Athens Area (Greece) Using the AirQ+ Model
Authors: Ntourou Kleopatra , Moustris Konstantinos , Spyropoulos Georgios , Fameli Kyriaki-Maria , Manousakis Nikolaos
Published: February 2023

It is well known that air pollution has a negative impact on human health. Research has shown an increasing trend in hospital admissions due to respiratory and heart diseases during and after consecutive days of high or even medium air pollution levels. The objective of this paper is to provide quantitative and qualitative data concerning the impact of long-term air pollution on the health of residents living in the Greater Athens Area (GAA). More accurately, the prevalence of bronchitis in children and the incidence of chronic bronchitis cases in adults due to particulate matter exposure are estimated utilizing the AirQ+ model. For this purpose, daily average concentrations of particulate matter with an aerodynamic diameter less than or equal to 10 μm (PM10) from five different locations within the GAA, covering the period 2001–2020, are used. The results show a significant correlation between PM10 concentrations and adverse health effects (R2 = 0.9). Interestingly, there were more cases of children suffering from bronchitis disease than cases of adults. In addition, it was observed that the unhealthiest areas in the GAA are the center of Athens city (mean annual PM10 concentration in 2019: 36 μgr/m3), as well as suburban areas (Lykovrissi and Marousi: mean annual PM10 concentrations in 2019 were 27 μgr/m3 and 28 μgr/m3, respectively). Finally, a decreasing trend for both PM10 concentrations and the prevalence of chronic bronchitis across the GAA was observed through the examined 20 years, which was significantly higher over the period 2010–2020.
Performance of the WRF Model for the Forecasting of the V-Shaped Storm Recorded on 11–12 November 2019 in the Eastern Sicily
Authors: Castorina Giuseppe , Semprebello Agostino , Insinga Vincenzo , Italiano Francesco , Caccamo Maria Teresa , Magazù Salvatore , Morichetti Mauro , Rizza Umberto
Published: February 2023

During the autumn season, Sicily is often affected by severe weather events, such as self-healing storms called V-shaped storms. These phenomena cause significant total rainfall quantities in short time intervals in localized spatial areas. In this framework, this study analyzes the meteorological event recorded on 11–12 November 2019 in Sicily (southern Italy), using the Weather Research and Forecasting (WRF) model with a horizontal spatial grid resolution of 3 km. It is important to note that, in this event, the most significant rainfall accumulations were recorded in eastern Sicily. In particular, the weather station of Linguaglossa North Etna (Catania) recorded a total rainfall of 293.6 mm. The precipitation forecasting provided by the WRF model simulation has been compared with the data recorded by the meteorological stations located in Sicily. In addition, a further simulation was carried out using the Four-Dimensional Data Assimilation (FDDA) technique to improve the model capability in the event reproduction. In this regard, in order to evaluate which approach provides the best performance (with or without FDDA), the Root Mean Square Error (RMSE) and dichotomous indexes (Accuracy, Threat Score, BIAS, Probability of Detection, and False Alarm Rate) were calculated.
Multi-Year Variations in Temperature in Mesopause Region and F2-Region Peak Electron Density over Eastern Siberia
Authors: Medvedeva Irina V. , Ratovsky Konstantin G.
Published: February 2023

We performed an analysis of year-to-year variations in the characteristics of the upper neutral atmosphere and the ionosphere over Eastern Siberia. The mesopause temperature (Tm) obtained from the spectrometric observations of the OH(6-2) emission and the peak electron density (NmF2) from the ionosonde measurements were used as atmospheric and ionospheric characteristics. We considered the annual mean Tm and yearly average values of NmF2, as well as yearly average values of day-to-day and intradiurnal variability in Tm and NmF2. To interpret the year-to-year variations, we use multiple regressions of the ionospheric and atmospheric characteristics on the F10.7-index (as a proxy of solar activity) and Ap-index (as a proxy of geomagnetic activity). For the atmospheric characteristics, we also used regressions on the SOI index (as a proxy of circulation in the lower atmosphere). The yearly average values of NmF2 are dominantly controlled by changes in the solar flux. The year-to-year variations in the NmF2 variability are mainly driven by changes in both solar and geomagnetic activity. The year-to-year variations in the mesopause temperature weakly correlate with changes in the indices of solar and geomagnetic activity. The yearly average values of Tm variability correlate with changes in the SOI-index: the day-to-day variability demonstrates a positive correlation with the SOI-index, while the intradiurnal variability shows a negative correlation with the SOI-index. The study did not reveal a significant relationship between the year-to-year variations in the NmF2 variability and Tm variability.
The Effects of Habitual Foot Strike Patterns on the Morphology and Mechanical Function of the Medial Gastrocnemius–Achilles Tendon Unit
Authors: Li Lu , Wu Kaicheng , Deng Liqin , Liu Cuixian , Fu Weijie
Published: February 2023

As a crucial and vulnerable component of the lower extremities, the medial gastrocnemius–Achilles tendon unit (gMTU) plays a significant role in sport performance and injury prevention during long-distance running. However, how habitual foot strike patterns influence the morphology of the gMTU remains unclear. Therefore, this study aimed to explore the effects of two main foot strike patterns on the morphological and mechanical characteristics of the gMTU. Long-distance male runners with habitual forefoot (FFS group, n = 10) and rearfoot strike patterns (RFS group, n = 10) and male non-runners (NR group, n = 10) were recruited. A Terason uSmart 3300 ultrasonography system was used to image the medial gastrocnemius (MG) and Achilles tendon, Image J software to analyze the morphology, and a dynamometer to determine plantar flexion torque during maximal voluntary isometric contractions. The participants first performed a 5-minute warm up; then, the morphological measurements of MG and AT were recorded in a static condition; finally, the MVICs test was conducted to investigate the mechanical function of the gMTU. One-way ANOVA and nonparametric tests were used for data analysis. The significance level was set at a p value of <0.05. The muscle fascicle length (FL) (FFS: 67.3 ± 12.7, RFS: 62.5 ± 7.6, NRs: 55.9 ± 2.0, η2 = 0.187), normalized FL (FFS: 0.36 ± 0.48, RFS: 0.18 ± 0.03, NRs: 0.16 ± 0.01, η2 = 0.237), and pennation angle (PA) (FFS: 16.2 ± 1.9, RFS: 18.9 ± 2.8, NRs: 19.3 ± 2.4, η2 = 0.280) significantly differed between the groups. Specifically, the FL and normalized FL were longer in the FFS group than in the NR group (p < 0.05), while the PA was smaller in the FFS group than in the NR group (p < 0.05). Conclusion: Long-term running with a forefoot strike pattern could significantly affect the FL and PA of the MG. A forefoot strike pattern could lead to a longer FL and a smaller PA, indicating an FFS pattern could protect the MG from strain under repetitive high loads.
Therapeutic Potential of Phytocannabinoid Cannabigerol for Multiple Sclerosis: Modulation of Microglial Activation In Vitro and In Vivo
Authors: Fleisher-Berkovich Sigal , Ventura Yvonne , Amoyal Maya , Dahan Arik , Feinshtein Valeria , Alfahel Leenor , Israelson Adrian , Bernstein Nirit , Gorelick Jonathan , Ben-Shabat Shimon
Published: February 2023

Multiple sclerosis (MS) is a widespread chronic neuroinflammatory and neurodegenerative disease. Microglia play a crucial role in the pathogenesis of MS via the release of cytokines and reactive oxygen species, e.g., nitric oxide. Research involving the role of phytocannabinoids in neuroinflammation is currently receiving much attention. Cannabigerol is a main phytocannabinoid, which has attracted significant pharmacological interest due to its non-psychotropic nature. In this research, we studied the effects of cannabigerol on microglial inflammation in vitro, followed by an in vivo study. Cannabigerol attenuated the microglial production of nitric oxide in BV2 microglia and primary glial cells; concomitant treatment of the cells with cannabigerol and telmisartan (a neuroprotective angiotensin receptor blocker) decreased nitric oxide production additively. Inducible nitric oxide synthase (iNOS) expression was also reduced by cannabigerol. Moreover, tumor necrosis factor-α (TNF-α), a major cytokine involved in MS, was significantly reduced by cannabigerol in both cell cultures. Next, we studied the effects of cannabigerol in vivo using a mice model of MS, experimental autoimmune encephalomyelitis (EAE). The clinical scores of EAE mice were attenuated upon cannabigerol treatment; additionally, lumbar sections of EAE mice showed enhanced neuronal loss (relative to control mice), which was restored by cannabigerol treatment. Altogether, the set of experiments presented in this work indicates that cannabigerol possesses an appealing therapeutic potential for the treatment of MS.
nWASP Inhibition Increases Wound Healing via TrKb/PLCγ Signalling
Authors: Frugtniet Bethan A. , Ruge Fiona , Sanders Andrew J. , Owen Sioned , Harding Keith G. , Jiang Wen G. , Martin Tracey A.
Published: February 2023

(1) Background: Chronic wounds represent a major burden to patients and healthcare systems and identifying new therapeutic targets to encourage wound healing is a significant challenge. This study evaluated nWASP as a new therapeutic target in human wound healing and determined how this can be regulated. (2) Methods: Clinical cohorts from patients with chronic wounds were tested for the expression of nWASP and cell models were employed to evaluate the influence of nWASP on cellular functions that are key to the healing process following knockdown and/or the use of nWASP-specific inhibitors. (3) Results: nWASP was significantly elevated at transcript levels in human non-healing chronic wounds versus healing tissues. nWASP inhibitors, wiskostatin and 187-1, along with the knockdown of nWASP, modified both HaCaT and HECV cell behaviour. We then identified two signalling pathways affected by nWASP inhibition: TrkB signalling and downstream PLCγ1 phosphorylation were impaired by nWASP inhibition in HaCaT cells. The healing of wounds in a diabetic murine model was significantly improved with an nWASP inhibitor treatment. (4) Conclusions: This study showed that nWASP activity was related to the non-healing behaviour of chronic wounds and together with the findings in the in vivo models, it strongly suggested nWASP as a therapeutic target in non-healing wounds that are regulated via TrkB and PLCγ1 signalling.
Low-Cost Impedance Camera for Cell Distribution Monitoring
Authors: Tang Bo , Liu Mengxi , Dietzel Andreas
Published: February 2023

Electrical impedance spectroscopy (EIS) is widely recognized as a powerful tool in biomedical research. For example, it allows detection and monitoring of diseases, measuring of cell density in bioreactors, and characterizing the permeability of tight junctions in barrier-forming tissue models. However, with single-channel measurement systems, only integral information is obtained without spatial resolution. Here we present a low-cost multichannel impedance measurement set-up capable of mapping cell distributions in a fluidic environment by using a microelectrode array (MEA) realized in 4-level printed circuit board (PCB) technology including layers for shielding, interconnections, and microelectrodes. The array of 8 × 8 gold microelectrode pairs was connected to home-built electric circuitry consisting of commercial components such as programmable multiplexers and an analog front-end module which allows the acquisition and processing of electrical impedances. For a proof-of-concept, the MEA was wetted in a 3D printed reservoir into which yeast cells were locally injected. Impedance maps were recorded at 200 kHz which correlate well with the optical images showing the yeast cell distribution in the reservoir. Blurring from parasitic currents slightly disturbing the impedance maps could be eliminated by deconvolution using an experimentally determined point spread function. The MEA of the impedance camera can in future be further miniaturized and integrated into cell cultivation and perfusion systems such as organ on chip devices to augment or even replace light microscopic monitoring of cell monolayer confluence and integrity during the cultivation in incubation chambers.
Functional Imaging and Inhibitor Screening of Human Pancreatic Lipase by a Resorufin-Based Fluorescent Probe
Authors: Hou Fan-Bin , Zhang Na , Zhu Guang-Hao , Fan Yu-Fan , Sun Meng-Ru , Nie Liang-Liang , Ge Guang-Bo , Zheng Yue-Juan , Wang Ping
Published: February 2023

Human pancreatic lipase (hPL) is a crucial digestive enzyme responsible for the digestion of dietary lipids in humans, and inhibition of hPL is effective in reducing triglyceride intake, thereby preventing and treating obesity. In this study, a series of fatty acids with different carbon chain lengths were constructed to the fluorophore resorufin based on the substrate preference of hPL. Among them, RLE was found to have the best combination of stability, specificity, sensitivity and reactivity towards hPL. Under physiological conditions, RLE can be rapidly hydrolyzed by hPL and released to resorufin, which triggered approximately 100-fold fluorescence enhancement at 590 nm. RLE was successfully applied for sensing and imaging of endogenous PL in living systems with low cytotoxicity and high imaging resolution. Moreover, a visual high-throughput screening platform was established using RLE, and the inhibitory effects of hundreds of drugs and natural products toward hPL were evaluated. Collectively, this study reports a novel and highly specific enzyme-activatable fluorogenic substrate for hPL that could serve as a powerful tool for monitoring hPL activity in complex biological systems and showcases the potential to explore physiological functions and rapid screening of inhibitors.
Recent Advancements in Novel Sensing Systems through Nanoarchitectonics
Authors: Velu Karthick , Shrestha Rekha Goswami , Shrestha Lok Kumar , Ariga Katsuhiko
Published: February 2023

The fabrication of various sensing devices and the ability to harmonize materials for a higher degree of organization is essential for effective sensing systems. Materials with hierarchically micro- and mesopore structures can enhance the sensitivity of sensors. Nanoarchitectonics allows for atomic/molecular level manipulations that create a higher area-to-volume ratio in nanoscale hierarchical structures for use in ideal sensing applications. Nanoarchitectonics also provides ample opportunities to fabricate materials by tuning pore size, increasing surface area, trapping molecules via host–guest interactions, and other mechanisms. Material characteristics and shape significantly enhance sensing capabilities via intramolecular interactions, molecular recognition, and localized surface plasmon resonance (LSPR). This review highlights the latest advancements in nanoarchitectonics approaches to tailor materials for various sensing applications, including biological micro/macro molecules, volatile organic compounds (VOC), microscopic recognition, and the selective discrimination of microparticles. Furthermore, different sensing devices that utilize the nanoarchitectonics concept to achieve atomic-molecular level discrimination are also discussed.
Applying Retinal Vascular Structures Characteristics Coupling with Cortical Visual System in Alzheimer’s Disease Spectrum Patients
Authors: Wang Lianlian , Hu Zheqi , Chen Haifeng , Sheng Xiaoning , Qin Ruomeng , Shao Pengfei , Yang Zhiyuan , Yao Weina , Zhao Hui , Xu Yun , Bai Feng
Published: February 2023

Cortical visual system dysfunction is closely related to the progression of Alzheimer’s Disease (AD), while retinal vascular structures play an important role in the integrity of the function of the visual network and are a potential biomarker of AD. This study explored the association between the cortical visual system and retinal vascular structures in AD-spectrum patients, and it established a screening tool to detect preclinical AD based on these parameters identified in a retinal examination. A total of 42 subjects were enrolled and were distributed into two groups: 22 patients with cognitive impairment and 20 healthy controls. All participants underwent neuropsychological tests, optical coherence tomography angiography and resting-state fMRI imaging. Seed-based functional connectivity analysis was used to construct the cortical visual network. The association of functional connectivity of the cortical visual system and retinal vascular structures was further explored in these subjects. This study found that the cognitive impairment group displayed prominently decreased functional connectivity of the cortical visual system mainly involving the right inferior temporal gyrus, left supramarginal gyrus and right postcentral gyrus. Meanwhile, we observed that retinal vascular structure characteristics deteriorated with the decline in functional connectivity in the cortical visual system. Our study provided novel insights into the aberrant cortical visual system in patients with cognitive impairment that strongly emphasized the critical role of retinal vascular structure characteristics, which could be used as potential biomarkers for diagnosing and monitoring the progression of AD.
Long-COVID in Patients with Cancer Previously Treated with Early Anti-SARS-CoV-2 Therapies in an Out-of-Hospital Setting: A Single-Center Experience
Authors: Lasagna Angioletta , Albi Giuseppe , Figini Simone , Basile Sara , Sacchi Paolo , Bruno Raffaele , Pedrazzoli Paolo
Published: February 2023

The incidence of long COVID in a cohort of patients with cancer with or without previous treatment with early therapies anti-SARS-CoV-2 in an out-of-hospital setting have to be elucidated. We prospectively enrolled all patients treated for a solid tumor at the department of Medical Oncology of the Fondazione IRCCS Policlinico San Matteo with a positive SARS-CoV-2 antigen or polymerase chain reaction test from January to September 2022 (Omicron surge). Ninety-seven patients answered the survey questions by telephone at least 12 weeks after COVID-19 diagnosis in order to evaluate the incidence of long COVID symptoms. Only twelve patients (12.4%) reported long COVID. No significant difference between early therapies anti-SARS-CoV-2 31 and long COVID (p = 0.443) was seen. The female sex (p = 0.024) and diabetes mellitus (p = 0.014) are significantly associated with long COVID. No statistically significant difference between the two groups (Long COVID vs. No Long COVID) according to the time to nasal swab viral clearance (p = 0.078). The overlap between the symptoms related to the oncological disease/oncological treatment and the symptoms of long COVID is one of the main future challenges that oncologists will have to manage.
Methane Dry Reforming over Ni/NiO Supported on Ce-, Zr-, and Al-Modified Y2O3 for Hydrogen Production
Authors: Chen Zijian , Mao Lei , Fang Xiuzhong , Xu Xianglan , Xu Junwei , Wang Xiang
Published: February 2023

In this work, Ce, Zr, and Al are used to promote Y2O3 as supports for Ni/NiO, with the expectation to obtain more efficient catalysts for DRM reaction. XRD and Raman results have testified that all the three cations have been doped into the lattice of Y2O3 to form a solid solution structure, thus obtaining supports with decreased crystallinity and improved surface areas. As a result, all the modified catalysts display evidently improved reaction performance. The Ni–support interaction of the modified catalysts is enhanced in comparison with the unmodified catalyst, thus having improved Ni dispersion. Moreover, the modified catalysts have improved alkalinity, which is beneficial to activate CO2 and enhance the activity. In addition, it is found that all the modified catalysts possess a richer amount of surface active oxygen species (O2δ− and O2−), which is critical to eliminate carbon depositions. It is believed that the interaction of these factors is responsible for the enhanced DRM performance of the modified catalysts. In situ DRIFTS results have confirmed that the addition of the secondary metals can improve the DRM activity of the catalyst by accelerating the conversion of formate intermediate species.
Cationic Covalent Triazine Network: A Metal-Free Catalyst for Effective Acetylene Hydrochlorination
Authors: Shen Zhaobing , Xing Ping , Wen Ke , Jiang Biao
Published: February 2023

Vinyl chloride, the monomer of polyvinyl chloride, is produced primarily via acetylene hydrochlorination catalyzed by environmentally toxic carbon-supported HgCl2. Recently, nitrogen-doped carbon materials have been explored as metal-free catalysts to substitute toxic HgCl2. Herein, we describe the development of a cationic covalent triazine network (cCTN, cCTN-700) that selectively catalyzes acetylene hydrochlorination. cCTN-700 exhibited excellent catalytic activity with initial acetylene conversion, reaching ~99% and a vinyl chloride selectivity of >98% at 200 °C during a 45 h test. X-ray photoelectron spectroscopy, temperature programmed desorption, and charge calculation results revealed that the active sites for the catalytic reaction were the carbon atoms bonded to the pyridinic N and positively charged nitrogen atoms (viologenic N+) of the viologen moieties in cCTN-700, similar to the active sites in Au-based catalysts but different from the those in previously reported nitrogen-doped carbon materials. This research focuses on using cationic covalent triazine polymers for selective acetylene hydrochlorination.
Glycative Stress, Glycated Hemoglobin, and Atherogenic Dyslipidemia in Patients with Hyperlipidemia
Authors: Yao Chien-An , Yen Tsung-Yi , Hsu Sandy Huey-Jen , Su Ta-Chen
Published: February 2023

(1) Background: Diabetes mellitus (DM) is a significant health problem and is associated with dyslipidemia; however, the association between glycative stress, in terms of glycated hemoglobin (HbA1c), and atherogenic dyslipidemia in hyperlipidemic patients with and without DM has rarely been reported. (2) Methods: We prospectively recruited 949 hyperlipidemic patients from the Lipid Clinic of the National Taiwan University Hospital. HbA1c and fasting serum lipids, including total cholesterol (TC), high- and low-density lipoprotein cholesterol (HDL-C and LDL-C), small dense LDL-C (sdLDL-C), very low-density lipoprotein cholesterol (VLDL-C), triglycerides, and advanced glycation end-products (AGEs), were measured. After fasting for 10–14 h, all subjects except those with DM underwent a standard oral glucose tolerance test (OGTT) with 75 g of glucose loading. All subjects were asked to discontinue the use of lipid-lowering agents for 8 weeks before recruitment. (3) Results: Patients with DM had a higher prevalence of hypertension and higher levels of triglyceride, TC/HDL-C ratio, AGEs, VLDL-C, and sdLDL-C. Among patients with higher HbA1c, the serum VLDL-C, AGEs, and TC/HDL-C ratio were significantly higher than those with lower HbA1c. After adjustment for covariates, multiple logistic regression analyses revealed different groups of dysglycemia with higher HbA1c had a higher odds ratio for TC/HDL-C ≥ 5, sdLDL-C ≥ 75th percentile, VLDL-C ≥ 75th percentile and AGEs ≥ 75th percentile. (4) Conclusions: A higher HbA1c was associated with a significant increase in the risk of atherogenic dyslipidemia and AGEs levels in patients with hyperlipidemia. The findings can be very promising in clinical application.
Congenital Microcephaly: A Debate on Diagnostic Challenges and Etiological Paradigm of the Shift from Isolated/Non-Syndromic to Syndromic Microcephaly
Authors: Asif Maria , Abdullah Uzma , Nürnberg Peter , Tinschert Sigrid , Hussain Muhammad Sajid
Published: February 2023

Congenital microcephaly (CM) exhibits broad clinical and genetic heterogeneity and is thus categorized into several subtypes. However, the recent bloom of disease–gene discoveries has revealed more overlaps than differences in the underlying genetic architecture for these clinical sub-categories, complicating the differential diagnosis. Moreover, the mechanism of the paradigm shift from a brain-restricted to a multi-organ phenotype is only vaguely understood. This review article highlights the critical factors considered while defining CM subtypes. It also presents possible arguments on long-standing questions of the brain-specific nature of CM caused by a dysfunction of the ubiquitously expressed proteins. We argue that brain-specific splicing events and organ-restricted protein expression may contribute in part to disparate clinical manifestations. We also highlight the role of genetic modifiers and de novo variants in the multi-organ phenotype of CM and emphasize their consideration in molecular characterization. This review thus attempts to expand our understanding of the phenotypic and etiological variability in CM and invites the development of more comprehensive guidelines.
Implications of Senescent Cell Burden and NRF2 Pathway in Uremic Calcification: A Translational Study
Authors: Laget Jonas , Hobson Sam , Muyor Karen , Duranton Flore , Cortijo Irene , Bartochowski Piotr , Jover Bernard , Lajoix Anne-Dominique , Söderberg Magnus , Ebert Thomas , Stenvinkel Peter , Argilés Àngel , Kublickiene Karolina , Gayrard Nathalie
Published: February 2023

Increased senescent cell burden and dysregulation of the nuclear factor erythroid 2–related factor 2 (NRF2) pathway have been associated with numerous age-related pathologies; however, their role in promoting vascular calcification (VC) in chronic kidney disease (CKD) has yet to be determined. We investigated whether senescence and NRF2 pathways may serve as drivers of uremia-induced VC using three complementary approaches: a novel model of induced VC in 5/6-nephrectomized rats supplemented with high phosphate and vitamin D; epigastric arteries from CKD patients with established medial calcification; and vascular smooth muscle cells (VSMCs) incubated with uremic serum. Expression of p16Ink4a and p21Cip1, as well as γ-H2A-positive cells, confirmed increased senescent cell burden at the site of calcium deposits in aortic sections in rats, and was similarly observed in calcified epigastric arteries from CKD patients through increased p16Ink4a expression. However, uremic serum-induced VSMC calcification was not accompanied by senescence. Expression of NRF2 and downstream genes, Nqo1 and Sod1, was associated with calcification in uremic rats, while no difference was observed between calcified and non-calcified EAs. Conversely, in vitro uremic serum-driven VC was associated with depleted NRF2 expression. Together, our data strengthen the importance of senescence and NRF2 pathways as potential therapeutic options to combat VC in CKD.
High Resolution Multiplex Confocal Imaging of the Neurovascular Unit in Health and Experimental Ischemic Stroke
Authors: Lochhead Jeffrey J. , Williams Erica I. , Reddell Elizabeth S. , Dorn Emma , Ronaldson Patrick T. , Davis Thomas P.
Published: February 2023

The neurovascular unit (NVU) is an anatomical group of cells that establishes the blood–brain barrier (BBB) and coordinates cerebral blood flow in association with neuronal function. In cerebral gray matter, cellular constituents of the NVU include endothelial cells and associated pericytes, astrocytes, neurons, and microglia. Dysfunction of the NVU is a common feature of diseases that affect the CNS, such as ischemic stroke. High-level evaluation of these NVU changes requires the use of imaging modalities that can enable the visualization of various cell types under disease conditions. In this study, we applied our confocal microscopy strategy using commercially available labeling reagents to, for the first time, simultaneously investigate associations between endothelial cells, the vascular basal lamina, pericytes, microglia, astrocytes and/or astrocyte end-feet, and neurites in both healthy and ischemic brain tissue. This allowed us to demonstrate ischemia-induced astrocyte activation, neurite loss, and microglial migration toward blood vessels in a single confocal image. Furthermore, our labeling cocktail enabled a precise quantification of changes in neurites and astrocyte reactivity, thereby showing the relationship between different NVU cellular constituents in healthy and diseased brain tissue. The application of our imaging approach for the simultaneous visualization of multiple NVU cell types provides an enhanced understanding of NVU function and pathology, a state-of-the-art advancement that will facilitate the development of more effective treatment strategies for diseases of the CNS that exhibit neurovascular dysfunction, such as ischemic stroke.
Long-Term Effect of Hyperbaric Oxygen Therapy on Gait and Functional Balance Skills in Cerebral Palsy Children—A Randomized Clinical Trial
Authors: Khalil Mohamed , Abdel Ghafar Mohamed , Abdelraouf Osama , Mohamed Mariam , Harraz Eman , Dawood Reem , Abouelkheir Reham
Published: February 2023

This study aimed to explore the long-term effects of hyperbaric oxygen therapy on spatiotemporal gait parameters and functional balance in children with cerebral palsy. Thirty-nine children with hemiplegic cerebral palsy were randomly allocated to one of two groups: control or study. The children in both groups received traditional physical therapy three times per week for six months. In addition, the children in the study group received hyperbaric oxygen therapy five times/week for eight weeks. The GAITRite system and pediatric balance scale were used to assess spatiotemporal gait parameters and functional balance at baseline, post-intervention, and six months after the cessation of hyperbaric oxygen therapy. Post-intervention means of all measured parameters were significantly higher than pre-intervention means, but only for the study group (p < 0.05). However, both groups’ means at the six-month follow up were significantly greater than those at pre-intervention (p < 0.05). At the post-intervention and follow-up evaluations, comparisons between groups revealed a statistically significant difference in all measured parameters for the study group against the control group (p < 0.05). It can be concluded that adding hyperbaric oxygen therapy to physical therapy rehabilitation could be effective in improving spatiotemporal gait parameters and functional balance in children with cerebral palsy.
Tribo- and Tribocorrosion Properties of Magnesium AZ31 Alloy
Authors: Mégret Alexandre , Prince Loïc , Olivier Marie-Georges , Vitry Véronique
Published: February 2023

In automotive and aerospace fields, the use of lightweight materials is required. Magnesium and its alloys combine a low density with high mechanical properties and excellent thermal conductivity. However, those materials suffer from low corrosion and wear resistances. The combined action of corrosion and wear is also critical for these materials. Tribocorrosion of magnesium alloy AZ31 has been investigated with reciprocal sliding wear as a function of the applied load in dry and wet (NaCl) conditions. The study shows that the main wear mechanisms were adhesion, abrasion, and oxidation in dry sliding wear while no adhesion was found in wet sliding wear. Corrosion of the worn surface occurs also in wet sliding wear. It is interesting to notice that wear is less pronounced in wet sliding wear than in dry sliding wear due to the natural lubrication of the NaCl electrolyte. Only severe conditions, high normal load, and wet conditions bring magnesium AZ31 alloy in filiform corrosion.
Clinical, Morphological, and Molecular Study on Grade 2 and 3 Pleomorphic Xanthoastrocytoma
Authors: Zhang Hui , Ma Xiao-Jing , Xiang Xue-Ping , Wang Qi-Yuan , Tang Jin-Long , Yu Xiao-Yan , Xu Jing-Hong
Published: February 2023

Purpose: Pleomorphic xanthoastrocytoma (PXA) is an uncommon astrocytoma that tends to occur in children and young adults and has a relatively favorable prognosis. The 2021 WHO classification of tumors of the central nervous system (CNS WHO), 5th edition, rates PXAs as grade 2 and grade 3. The histological grading was based on mitotic activity (≥2.5 mitoses/mm2). This study specifically evaluates the clinical, morphological, and, especially, the molecular characteristics of grade 2 and 3 PXAs. Methods: Between 2003 and 2021, we characterized 53 tumors with histologically defined grade 2 PXA (n = 36, 68%) and grade 3 PXA (n = 17, 32%). Results: Compared with grade 2 PXA, grade 3 PXA has a deeper location and no superiority in the temporal lobe and is more likely to be accompanied by peritumoral edema. In histomorphology, epithelioid cells and necrosis were more likely to occur in grade 3 PXA. Molecular analysis found that the TERT promoter mutation was more prevalent in grade 3 PXA than in grade 2 PXA (35% vs. 3%; p = 0.0005) and all mutation sites were C228T. The cases without BRAF V600E mutation or with necrosis in grade 3 PXA had a poor prognosis (p = 0.01). Conclusion: These data define PXA as a heterogeneous astrocytoma. Grade 2 and grade 3 PXAs have different clinical and histological characteristics as well as distinct molecular profiles. TERT promoter mutations may be a significant genetic event associated with anaplastic progression. Necrosis and BRAF V600E mutation play an important role in the prognosis of grade 3 PXA.
Performance of T-Track® TB, a Novel Dual Marker RT-qPCR-Based Whole-Blood Test for Improved Detection of Active Tuberculosis
Authors: Meier Johannes P. , Möbus Selina , Heigl Florian , Asbach-Nitzsche Alexandra , Niller Hans Helmut , Plentz Annelie , Avsar Korkut , Heiß-Neumann Marion , Schaaf Bernhard , Cassens Uwe , Seese Bernd , Teschner Daniel , Handzhiev Sabin , Graf Uwe , Lübbert Christoph , Steinmaurer Monika , Kontogianni Konstantina , Berg Christoph , Maieron Andreas , Blaas Stefan H. , Wagner Ralf , Deml Ludwig , Barabas Sascha
Published: February 2023

Tuberculosis (TB) is one of the leading causes of death by an infectious disease. It remains a major health burden worldwide, in part due to misdiagnosis. Therefore, improved diagnostic tests allowing the faster and more reliable diagnosis of patients with active TB are urgently needed. This prospective study examined the performance of the new molecular whole-blood test T-Track® TB, which relies on the combined evaluation of IFNG and CXCL10 mRNA levels, and compared it to that of the QuantiFERON®-TB Gold Plus (QFT-Plus) enzyme-linked immunosorbent assay (ELISA). Diagnostic accuracy and agreement analyses were conducted on the whole blood of 181 active TB patients and 163 non-TB controls. T-Track® TB presented sensitivity of 94.9% and specificity of 93.8% for the detection of active TB vs. non-TB controls. In comparison, the QFT-Plus ELISA showed sensitivity of 84.3%. The sensitivity of T-Track® TB was significantly higher (p < 0.001) than that of QFT-Plus. The overall agreement of T-Track® TB with QFT-Plus to diagnose active TB was 87.9%. Out of 21 samples with discordant results, 19 were correctly classified by T-Track® TB while misclassified by QFT-Plus (T-Track® TB-positive/QFT-Plus-negative), and two samples were misclassified by T-Track® TB while correctly classified by QFT-Plus (T-Track® TB-negative/QFT-Plus-positive). Our results demonstrate the excellent performance of the T-Track® TB molecular assay and its suitability to accurately detect TB infection and discriminate active TB patients from non-infected controls.