bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Additivized Biodiesel Blends on Diesel Engine Performance, Emission, Tribological Characteristics, and Lubricant Tribology
Authors: Mujtaba M. A. , Masjuki H. H. , Kalam M. A. , Noor Fahad , Farooq Muhammad , Ong Hwai Chyuan , Gul M. , Soudagar Manzoore Elahi M. , Bashir Shahid , Rizwanul Fattah I. M. , Razzaq L.
Published: July 2020

This research work focuses on investigating the lubricity and analyzing the engine characteristics of diesel–biodiesel blends with fuel additives (titanium dioxide (TiO2) and dimethyl carbonate (DMC)) and their effect on the tribological properties of a mineral lubricant. A blend of palm–sesame oil was used to produce biodiesel using ultrasound-assisted transesterification. B30 (30% biodiesel + 70% diesel) fuel was selected as the base fuel. The additives used in the current study to prepare ternary fuel blends were TiO2 and DMC. B30 + TiO2 showed a significant reduction of 6.72% in the coefficient of friction (COF) compared to B30. B10 (Malaysian commercial diesel) exhibited very poor lubricity and COF among all tested fuels. Both ternary fuel blends showed a promising reduction in wear rate. All contaminated lubricant samples showed an increment in COF due to the dilution of combustible fuels. Lub + B10 (lubricant + B10) showed the highest increment of 42.29% in COF among all contaminated lubricant samples. B30 + TiO2 showed the maximum reduction (6.76%) in brake-specific fuel consumption (BSFC). B30 + DMC showed the maximum increment (8.01%) in brake thermal efficiency (BTE). B30 + DMC exhibited a considerable decline of 32.09% and 25.4% in CO and HC emissions, respectively. The B30 + TiO2 fuel blend showed better lubricity and a significant improvement in engine characteristics.
Force Feedback Assistance in Remote Ultrasound Scan Procedures
Authors: Bucolo Maide , Buscarino Arturo , Fortuna Luigi , Gagliano Salvina
Published: July 2020

In this contribution, we approached a new aspect in robotic applications. We investigated human–machine modeling for remote ultrasound scan equipment. While robotic systems for ultrasound scan applications with remote operations have been widely studied, in this research, remote force-feedback control was tested. The goal is for the human operator to receive, as physical input, the correct force perception transmitted by the remote ultrasound scan equipment in analyzing the body of the patient. Two principal aspects were investigated. The first was an artificial body model to receive the control signals from the remote equipment. The second aspect was to study a suitable feedback control law that attempts to compensate for the uncertainty between the artificial body and the patient’s body, while also taking into account the transmission delay. Therefore, the task was to give the operator relevant information while considering the force effect; thus, providing a reliable and efficient platform in order to work in remote conditions with ultrasound scan equipment.
Theoretical and Economic Evaluation of Low-Cost Deep Eutectic Solvents for Effective Biogas Upgrading to Bio-Methane
Authors: Słupek Edyta , Makoś Patrycja , Gębicki Jacek
Published: July 2020

This paper presents the theoretical screening of 23 low-cost deep eutectic solvents (DESs) as absorbents for effective removal of the main impurities from biogas streams using a conductor-like screening model for real solvents (COSMO-RS). Based on thermodynamic parameters, i.e., the activity coefficient, excess enthalpy, and Henry’s constant, two DESs composed of choline chloride: urea in a 1:2 molar ratio (ChCl:U 1:2), and choline chloride: oxalic acid in a 1:2 molar ratio (ChCl:OA 1:2) were selected as the most effective absorbents. The σ-profile and σ-potential were used in order to explain the mechanism of the absorptive removal of CO2, H2S, and siloxanes from a biogas stream. In addition, an economic analysis was prepared to demonstrate the competitiveness of new DESs in the sorbents market. The unit cost of 1 m3 of pure bio-methane was estimated to be in the range of 0.35–0.37 EUR, which is comparable to currently used technologies.
Dimensioning Methodology of Energy Storage Systems for Power Smoothing in a Wave Energy Conversion Plant Considering Efficiency Maps and Filtering Control Techniques
Authors: Torres Jorge , Blanco Marcos , Lafoz Marcos , Navarro Gustavo , Nájera Jorge , Santos-Herran Miguel
Published: July 2020

This paper aims at presenting and describing a dimensioning methodology for energy storage systems (ESS), in particular for one based on flywheels, applied for the specific application of reducing power oscillation in a wave energy conversion (WEC) plant. The dimensioning methodology takes into account the efficiency maps of the storage technology as well as the effect of the filtering control techniques. The methodology is applied to the case study of a WEC plant in operation in Spain, using real power generation profiles delivered into the electric grid. The paper firstly describes the calculation procedure of the efficiency maps for the particular technology of flywheels, although it could be extended to other storage technologies. Then, the influence of using future data values in the dimensioning process and the control of the ESS operation is analysed in depth. A moving average filter (MAF) is defined to compensate for power oscillations, studying the difference between considering prediction and not doing so. It is concluded that a filtering control using future values based on a number of samples equivalent to a 4-min time order provides an important reduction in the energy storage requirements for a power generation plant. Finally, and based on the selection of storage modules previously defined, the efficiency maps, and the MAF used for delivering the power into the grid, an optimal operation strategy is suggested for the storage modules, based on a stepped switching technique. The selection of four flywheel energy storage system (FESS) modules achieves a reduction of 50% in power oscillations, covering 85% of the frequency excursions at the grid.
Grid Synchronization and Islanding Detection Methods for Single-Stage Photovoltaic Systems
Authors: Mastromauro Rosa Anna
Published: July 2020

Synchronization and islanding detection represent some of the main issues for grid-connected photovoltaic systems (PVSs). The synchronization technique allows to achieve PVS high power factor operation and it provides grid voltage monitoring. The islanding detection control function ensures safe operation of the PVS. Focusing on low-power single-stage PVSs, in this study the most adopted and the highest performance synchronization and islanding detection methods are discussed. The role of the synchronization system is fundamental to detect the grid conditions, for the islanding detection purpose, and to manage the reconnection to the grid after a PVS trip. Hence a combined review is advantageous.
Towards Self-Sustainable Island Grids through Optimal Utilization of Renewable Energy Potential and Community Engagement
Authors: Jelić Marko , Batić Marko , Tomašević Nikola , Barney Andrew , Polatidis Heracles , Crosbie Tracey , Abi Ghanem Dana , Short Michael , Pillai Gobind
Published: July 2020

Solving the issue of energy security for geographical islands presents a one-of-a-kind problem that has to be tackled from multiple sides and requires an interdisciplinary approach that transcends just technical and social aspects. With many islands suffering in terms of limited and costly energy supply due to their remote location, providing a self-sustainable energy system is of utmost importance for these communities. In order to improve upon the status quo, novel solutions and projects aimed at increasing sustainability not only have to consider optimal utilization of renewable energy potentials in accordance with local conditions, but also must include active community participation. This paper analyzes both of these aspects for island communities and brings them together in an optimization scenario that is utilized to determine the relationship between supposed demand flexibility levels and achievable savings in a setting with variable renewable generation. The results, specifically discussed for a use case with real-world data for the La Graciosa island in Spain, show that boosting community participation and thus unlocking crucial demand flexibility, can be used as a powerful tool to augment novel generation technologies with savings from flexibility at around 7.5% of what is achieved purely by renewable sources.
Mapping Fuzzy Energy Management Strategy for PEM Fuel Cell–Battery–Supercapacitor Hybrid Excavator
Authors: Truong Hoai Vu Anh , Dao Hoang Vu , Do Tri Cuong , Ho Cong Minh , To Xuan Dinh , Dang Tri Dung , Ahn Kyoung Kwan
Published: July 2020

By replacing conventional supplies such as fossil fuels or internal combustion engines (ICEs), this paper presents a new configuration of hybrid power sources (HPS) based on the integration of a proton-exchange membrane fuel cell (PEMFC) with batteries (BATs) and supercapacitors (SCs) for hydraulic excavators (HEs). In contrast to conventional architectures, the PEMFC in this study functions as the main power supply, whereas the integrated BAT–SC is considered as an auxiliary buffer. Regarding shortcomings existing in the previous approaches, an innovative energy management strategy (EMS) was designed using a new mapping fuzzy logic control (MFLC) for appropriate power distribution. Comparisons between the proposed strategy with available approaches are conducted to satisfy several driving cycles with different load demands and verify the strategy’s effectiveness. Based on the simulation results, the efficiency of the PEMFC when using the MFLS algorithm increased up to 47% in comparison with the conventional proposed EMS and other approaches. With the proposed strategy, the HPS can be guaranteed to not only sufficiently support power to the system even when the endurance process or high peak power is required, but also extend the lifespan of the devices and achieves high efficiency.
Thermodynamic Optimization of a Waste Heat Power System under Economic Constraint
Authors: Ren Liya , Liu Jianyu , Wang Huaixin
Published: July 2020

A novel thermo-economic performance indicator for a waste heat power system, namely, MPC, is proposed in this study, which denotes the maximum net power output with the constraint of EPC ≤ EPC0, where EPC is the electricity production cost of the system and EPC0 refers to the EPC of conventional fossil fuel power plants. The organic and steam Rankine cycle (ORC, SRC) systems driven by the flue gas are optimized to maximize the net power output with the constraint of EPC ≤ EPC0 by using the Non-dominated Sorting Genetic Algorithm-II (NSGA-II). The optimization process entails the design of the heat exchangers, the instantaneous calculation of the turbine efficiency, and the system cost estimation based on the Aspen Process Economic Analyzer. Six organic fluids, n-butane, R245fa, n-pentane, cyclo-pentane, MM (Hexamethyldisiloxane), and toluene, are considered for the ORC system. Results indicate that the MPC of the ORC system using cyclo-pentane is 39.7% higher than that of the SRC system under the waste heat source from a cement plant with an initial temperature of 360 °C and mass flow rate of 42.15 kg/s. The precondition of the application of the waste heat power system is EPC ≤ EPC0, and the minimum heat source temperatures to satisfy this condition for ORC and SRC systems are obtained. Finally, the selection map of ORC versus SRC based on their thermo-economic performance in terms of the heat source conditions is provided.
Biomass Availability in Europe as an Alternative Fuel for Full Conversion of Lignite Power Plants: A Critical Review
Authors: Tzelepi Vasiliki , Zeneli Myrto , Kourkoumpas Dimitrios-Sotirios , Karampinis Emmanouil , Gypakis Antonios , Nikolopoulos Nikos , Grammelis Panagiotis
Published: July 2020

Biomass has been demonstrated as a capable source of energy to fulfill the increasing demand for clean energy sources which could last a long time. Replacing fossil fuels with biomass-based ones can potentially lead to a reduction of carbon emissions, which is the main target of the EU climate strategy. Based on RED II (revised Renewable Energy Directive 2018/2001/EU) and the European Green Deal, biomass is a promising energy source for achieving carbon neutrality in the future. However, the sustainable potential of biomass resources in the forthcoming decades is still a matter of question. This review aims at estimating the availability of biomass for energy reasons in the EU, and to evaluate its potential to meet the coal power plant capacity of the main lignite-producer countries, including Germany, Poland and Greece. Plants in line with the sustainability criteria of RED II have been selected for the preliminary estimations concerning their full conversion to the biomass power concept. Furthermore, the various barriers to biomass utilization are highlighted, such as the stranded asset risk of a future coal phase-out scenario, biomass supply chain challenges, biomass availability in main lignite-producer EU countries, the existing full conversion technologies, and biomass cost. A variety of challenges in the scenario of lignite substitution with biomass in a plant are investigated in a SWOT (strengths, weaknesses, opportunities, and threats) analysis. Technological risks and issues should be tackled in order to achieve the coal phase-out EU goal, mainly with regard to the supply chain of biomass. In this direction, the development of logistics centers for the centralized handling of biomass is strongly recommended.
Performance Indexes of an Air-Water Heat Pump Versus the Capacity Ratio: Analysis by Means of Experimental Data
Authors: Bruno Roberto , Nicoletti Francesco , Cuconati Giorgio , Perrella Stefania , Cirone Daniela
Published: July 2020

The spread of near-Zero Energy Buildings (nZEB) involves the employment of high performant air-conditioning plants where renewable sources can be integrated easily. In this context, heat pumps appear as a promising solution given their ability to exploit aerothermal, hydrothermal and geothermal sources and to supply both heating and cooling loads with the same device. In order to evaluate the energy performances in transient conditions, the actual winter (COP) and summer (EER) performance indexes, in the function of the sources’ temperatures and the capacity ratio (CR), have to be available. Nevertheless, heat pump manufactures often provide the trend of the performance indexes in the function of the temperatures of the sources specifically for nominal conditions, whereas the dependence of the performance indexes in the function of CR, that takes into account the part-load operation, is almost always not provided. Alternatively, specific technical standards suggest the use of a correction factor to modify nominal COP and EER for the attainment of the real performance indexes. In this paper, by using data from an experimental set-up equipped with air-water heat pumps, these correlations were tested and tuned. Winter results showed that correction factors suggested by standards have to be modified in the presence of a storage system. In summer, instead, a new correlation was developed to find a function between nominal and actual EERs in the function of CR by exploiting a similar approach employed for the COP calculation.
Regulatory Effect of Lactobacillus brevis Bmb6 on Gut Barrier Functions in Experimental Colitis
Authors: Shin Mi-Young , Yong Cheng-Chung , Oh Sejong
Published: July 2020

The integrity of gut barrier functions is closely associated with the pathogenesis of colitis. It is speculated that Lactobacillus brevis Bmb6 alleviates colitis by improving the tight junction (TJ) of the inflamed intestinal epithelial layer. In the present study, the regulatory effects of L. brevis Bmb6 on the TJ barrier to ameliorate colitis-symptoms were investigated. Preliminary screening showed that L. brevis Bmb6 exhibited strong acid and bile acid tolerance, along with antioxidants and β-galactosidase activities. In a 14-day dextran sulfate sodium (DSS)-induced colitis mouse model, treatment with L. brevis Bmb6 significantly decreased in the disease activity index score. In addition, histological analyses showed that treatment with L. brevis Bmb6 protected the structural integrity of the intestinal epithelial layer and mucin-secreting goblet cells from DSS-induced damage, with only slight infiltration of immune cells. Interestingly, western blotting analyses showed that the expression of the TJ protein, zona occluden-1, was restored in Bmb6-treated mice, but not in DSS-induced mice. Consistently, the gene expression of inflammatory cytokines (tumor necrosis factor-α and interferon-γ) was also suppressed in the Bmb6-treated mice. Hence, our findings suggest that suppression of inflammatory conditions enhanced expression of TJ protein, ZO-1, or vice versa, contributing to a colitis-ameliorating effect in L. brevis Bmb6.
Pro-Dominion Attitudes toward Nature in Western Culture: First Cracks in the Narrative
Authors: Nir Bina
Published: July 2020

Our civilization’s interference in nature is the source of numerous ecological problems. This study will employ a genealogical methodology to examine the “man’s dominion over nature” approach, which is deeply rooted in Western culture. The underlying aim of genealogical research is to understand contemporary reality by means of the reinterpretation of the past. Through this new interpretation, we will reveal the deep religious and cultural foundations, grounded in Judeo-Christian monotheism, of the pro-dominion attitude to nature. This article’s genealogical-exegetical analysis of central religious texts aims to contribute to our cultural understanding of the present. Deeply rooted constructs, originating in religious life, tend to remain in the culture even after its secularization. Following our examining the roots of the concept and the mental constructs that it created, we will turn to consider the first cracks in this ancient narrative. A close consideration of the development of these cracks has the potential to spur profound cultural change.
Brain Protection in the Endo-Management of Proximal Aortic Aneurysms
Authors: Hanna Lydia , Gibbs Richard
Published: July 2020

Neurological brain injury (NBI) remains the most feared complication following thoracic endovascular aortic repair (TEVAR), and can manifest as clinically overt stroke and/or more covert injury, detected only on explicit neuropsychological testing. Microembolic signals (MES) detected on transcranial Doppler (TCD) monitoring of the cerebral arteries during TEVAR and the high prevalence and incidence of new ischaemic infarcts on diffusion-weighted magnetic resonance imaging (DW-MRI) suggests procedure-related solid and gaseous cerebral microembolisation to be an important cause of NBI. Any intervention that can reduce the embolic burden during TEVAR may, therefore, help mitigate the risk of stroke and the covert impact of ischaemic infarcts to the function of the brain. This perspective article provides an understanding of the mechanism of stroke and reviews the available evidence regarding potential neuroprotective strategies that target high-risk procedural steps of TEVAR to reduce periprocedural cerebral embolisation.
Breast Cancer Primary Prevention and Diet: An Umbrella Review
Authors: Buja Alessandra , Pierbon Marco , Lago Laura , Grotto Giulia , Baldo Vincenzo
Published: July 2020

Introduction: Many studies have been published, but none have pooled the useful evidence available in the literature to produce guidelines and health policies promoting healthy eating styles to prevent breast cancer (BC). The present study aimed to summarize the evidence produced to date, taking a judicious, critical approach to the quality of the studies analyzed. Methods: An umbrella review method was adopted, which is a systematic review of second-level studies, meta-analyses and literature reviews. Results: In all, 48 studies were considered: 32 meta-analyses, 4 pooled analyses, 5 systematic reviews, and 7 qualitative reviews. A higher intake of total meat, or red or processed meats, or foods with a high glycemic index, or eggs would seem to be associated with a higher risk of BC. Some foods, such as vegetables, would seem instead to have an inverse association with BC risk. One meta-analysis revealed an inverse association between citrus fruit and mushroom consumption and BC. Some nutrients, such as calcium, folate, vitamin D, lignans and carotenoids, also seem to be inversely associated with BC risk. The evidence is still conflicting as concerns exposure to other dietary elements (e.g., polyunsaturated fatty acids, dairy foods). Conclusion: Nutrition is one of the most modifiable aspects of people’s lifestyles and dietary choices can affect health and the risk of cancer. Overall, adhering to a healthy eating style may be associated with a significant reduction in the risk of BC.
Effects of Exercise Training during Christmas on Body Weight and Cardiometabolic Health in Overweight Individuals
Authors: Ramirez-Jimenez Miguel , Morales-Palomo Felix , Ortega Juan Fernando , Moreno-Cabañas Alfonso , Guio de Prada Valle , Alvarez-Jimenez Laura , Mora-Rodriguez Ricardo
Published: July 2020

Individuals with abdominal obesity and metabolic syndrome (MetS) have augmented risk of all-cause mortality. Lifestyle interventions are effective to treat MetS, however, there are periods during the year in which exercise programs are discontinued and improper dietary habits reappear (e.g., Christmas holidays). We aimed to analyze if exercise-training during Christmas holidays would avoid body-weight gains and cardiometabolic deterioration in MetS individuals, using a randomized control trial. Thirty-eight men with MetS undergoing exercise training were randomly allocated to either continue (TRAIN group, n = 16) or discontinue (HOLID group, n = 22) training, during the three weeks of Christmas. Anthropometrics (body weight, fat, and waist circumference), fasting blood metabolites (glucose, insulin, triglycerides, and cholesterol concentrations) and exercise maximal fat oxidation (FOMAX) and oxygen uptake (VO2PEAK) were determined before and after Christmas. Both groups were similar at baseline in all parameters (p > 0.05). HOLID group increased body weight (91.3 ± 13.0 to 92.0 ± 13.4 kg, p = 0.004), mean arterial pressure (94.0 ± 10.6 to 97.1 ± 8.9 mmHg, p = 0.026), blood insulin (10.2 ± 3.8 to 12.5 ± 5.4 µIU·mL−1, p = 0.003) and HOMA (3.2 ± 1.3 to 4.1 ± 2.3, p = 0.003). In contrast, TRAIN prevented those disarrangements and reduced total (170.6 ± 30.6 to 161.3 ± 31.3 mg·dL−1, p = 0.026) and low-density lipoprotein cholesterol (i.e., LDL-C, 104.8 ± 26.1 to 95.6 ± 21.7 mg·dL−1, p = 0.013). TRAIN also prevented the reductions in exercise FOMAX and VO2PEAK that was observed in the HOLID group (p = 0.002). In conclusion, exercise training during Christmas, prevents body weight gains and the associated cardiovascular (increase in blood pressure and LDL-C) and metabolic (reduced insulin sensitivity) health risks are an optimal non-pharmacological therapy for that period of the year.
Sedentariness and Physical Activity during School Recess Are Associated with VO2Peak
Authors: Calahorro-Cañada Fernando , Torres-Luque Gema , López-Fernández Iván , Carnero Elvis A.
Published: July 2020

Recess time (RT) is a main component of school-based activities, and could contribute up to 40% of the physical activity (PA) recommended in the health guidelines. The main goal was to analyze the association between accelerometer-measured PA and sedentary time during RT with cardiorespiratory fitness (CRF). A total of 146 children and adolescents, aged 8–19 years, were recruited from six schools. PA levels were measured with GT3X accelerometers over 7 days. CRF was measured using a portable breath-by-breath gas analyzer. A general linear model (GML) was conducted to analyze the association between PA intensities and CRF during RT. Additionally, a logistic binary regression was used to evaluate the risk of unhealthy CRF among different categories of PA and sedentary time. Participants classified as healthy showed higher PA levels during RT than those classed as unhealthy. GML analysis showed that sedentary time during RT was significantly associated with VO2Peak. Finally, compared to individuals accumulate less than 15-min of ST during recess, individuals who were sedentary for more than 15 min during RT presented 43.78 times of having unhealthy CRF (95% CI 3.873–494.824). Our data suggest an association between recess sedentary time and unhealthy CRF. Thus, school-aged children and adolescents must be empowered to perform PA during RT to prevent the deleterious effects of sedentary time on CRF.
How Does Mission Statement Relate to the Pursuit of Food Safety Certification by Food Companies?
Authors: Lin Quan , Zhu Yutao , Zhang Yue
Published: July 2020

Food safety has long been a major public concern in China. One question of the food processing industry’s emphasis on food safety social responsibility is whether a food processing company should pursue food safety certification for its products. As part of their corporate image, some food processing companies focus on food safety in their corporate mission statements. To enhance the legitimacy of a mission statement, as a guide for a firm, can provide food companies the legitimacy of perhaps pursuing food safety certification. However, we find that under different equity natures, the pressures on the normative legitimacy of the firm are different and the impact of mission statements on the acquisition of food safety certifications is also different. By analyzing the mission statement of companies in the Chinese food industry, we find that firms with a mission focusing on food safety concerns are more willing to pursue food safety certification. Moreover, compared to the firms with more distributed shareholder ownership, in firms where a majority shareholder has substantial control, the relationship between mission statements and the possession of food safety certification is stronger; compared to non-state-owned enterprises, in state-owned enterprise (SOEs), the relationship between firm mission statements of and the acquisition of food safety certification is stronger.
Canadian Public Safety Personnel and Occupational Stressors: How PSP Interpret Stressors on Duty
Authors: Ricciardelli Rosemary , Czarnuch Stephen , Carleton R. Nicholas , Gacek James , Shewmake James
Published: July 2020

Canadian public safety personnel (e.g., correctional workers, firefighters) experience potential stressors as a function of their occupation. Occupational stressors can include organizational (e.g., job context) and operational (e.g., job content) elements. Operational stressors (e.g., exposures to potentially psychologically traumatic events) may be inevitable, but opportunities may exist to mitigate other occupational stressors for public safety personnel. Research exploring the diverse forms of stress among public safety personnel remains sparse. In our current qualitative study we provide insights into how public safety personnel interpret occupational stressors. We use a semi-grounded thematic approach to analyze what public safety personnel reported when asked to further comment on occupational stress or their work experiences in two open-ended comment fields of an online survey. We provide a more comprehensive understanding of how public safety personnel experience occupational stress and the stressors that are unique to their occupations. Beyond known operational stressors, our respondents (n = 1238; n = 828) reported substantial difficulties with organizational (interpersonal work relationship dynamics; workload distribution, resources, and administrative obligations) and operational (vigilance, work location, interacting with the public) stressors. Some operational stressors are inevitable, but other occupational stressors can be mitigated to better support our public safety personnel.
Forecasting Weekly Influenza Outpatient Visits Using a Two-Dimensional Hierarchical Decision Tree Scheme
Authors: Lee Tian-Shyug , Chen I-Fei , Chang Ting-Jen , Lu Chi-Jie
Published: July 2020

Influenza is a serious public health issue, as it can cause acute suffering and even death, social disruption, and economic loss. Effective forecasting of influenza outpatient visits is beneficial to anticipate and prevent medical resource shortages. This study uses regional data on influenza outpatient visits to propose a two-dimensional hierarchical decision tree scheme for forecasting influenza outpatient visits. The Taiwan weekly influenza outpatient visit data were collected from the national infectious disease statistics system and used for an empirical example. The 788 data points start in the first week of 2005 and end in the second week of 2020. The empirical results revealed that the proposed forecasting scheme outperformed five competing models and was able to forecast one to four weeks of anticipated influenza outpatient visits. The scheme may be an effective and promising alternative for forecasting one to four steps (weeks) ahead of nationwide influenza outpatient visits in Taiwan. Our results also suggest that, for forecasting nationwide influenza outpatient visits in Taiwan, one- and two-time lag information and regional information from the Taipei, North, and South regions are significant.
The Association of Parental Interest in Entrepreneurship with the Entrepreneurial Interest of Spanish Youth
Authors: Luis-Rico María-Isabel , Escolar-Llamazares María-Camino , de la Torre-Cruz Tamara , Herrero Álvaro , Jiménez Alfredo , Arranz Val Pablo , Palmero-Cámara Carmen , Jiménez-Eguizábal Alfredo
Published: July 2020

As entrepreneurial interest is believed to represent a causal factor increasing entrepreneurship, research has begun to explore how family systems affect youth entrepreneurial interests. In the present study, we attempt to identify different types of family influence on the entrepreneurial interests of young people. A questionnaire was used to obtain data from 1633 Spanish adolescents (15 to 18 years old) and another questionnaire was used to obtain data from 839 parents. Principal component analysis identified unique family types and revealed that they have differential associations to entrepreneurial interest among youth. These findings reaffirm the influence of family on the entrepreneurial ecosystem and the promotion of an entrepreneurial family culture. This study further suggests that early attention should focus on the detection of entrepreneurial interest among youth so that actions can be implemented in the families to incentivize an entrepreneurial family culture.
How Servant Leadership Motivates Innovative Behavior: A Moderated Mediation Model
Authors: Zeng Jianji , Xu Guangyi
Published: July 2020

Drawing on social identity theory, this study examines the effect of servant leadership on university teachers’ innovative behavior through the self-concept constructs of perceived insider status and organization-based self-esteem, and the moderating effect of leader–member exchange (LMX). This moderated mediation model was tested with two waves of data from 269 university teachers in China. Results reveal that the self-concept constructs mediate the relationship between servant leadership and university teachers’ innovative behavior. Moreover, LMX strengthens the relationship between servant leadership and the self-concept constructs, as well as the indirect effect of servant leadership on university teachers’ innovative behavior through the self-concept constructs. Findings suggest that servant leadership is related to increased innovative behavior due to its positive influence on the self-concept of university teachers and it highlights the importance of developing a favorable supervisor–subordinate relationship.
Functional Recognition by CD8+ T Cells of Epitopes with Amino Acid Variations Outside Known MHC Anchor or T Cell Receptor Recognition Residues
Authors: Wilson Kirsty L. , Xiang Sue D. , Plebanski Magdalena
Published: July 2020

Peptide-based vaccines can be safer and more cost effective than whole organism vaccines. Previous studies have shown that inorganic polystyrene nanoparticles (PSNPs) covalently conjugated to the minimal immunodominant peptide epitope from murine liver stage malaria (SYIPSAEKI) induced potent CD8+ T cell responses. Many pathogens, including malaria, have polymorphic T cell epitope regions. Amino acid changes in positions that are contact residues for the T cell receptor (TCR) often alter the specific cross-reactivity induced by the peptide antigen, and it is largely assumed that changes outside of these residues have little impact. Herein, each amino acid residue (except major histocompatibility complex (MHC) anchors) was systematically changed to an alanine. Peptide epitopes with altered amino acids outside T cell contact residues were still recognized by T cells induced by PSNPs-SYIPSAEKI (KI) vaccines, albeit at lower levels, except for the variant SYIPSAAKI (A7). PSNPs-SYIPSAAKI vaccines further elicited high responses to the index KI peptide. None of the epitopes displayed altered peptide ligand (APL) antagonism in vitro, and re-stimulating SYIPSAEKI and SYIPSAAKI together synergistically enhanced IFN-γ production by the T cells. These results show epitope variation in non-TCR recognition residues can have effects on T cell reactivity, suggesting that such natural variation may also be driven by immune pressure. Additionally, when re-modelling peptides to enhance the cross-reactivity of vaccines, both TCR recognition and non-recognition residues should be considered.
Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata
Authors: Xiong Jiang , Liu Lian , Ma Xiaochuan , Li Feifei , Tang Chaolan , Li Zehang , Lü Biwen , Zhou Tie , Lian Xuefei , Chang Yuanyuan , Tang Mengjing , Xie Shenxi , Lu Xiaopeng
Published: July 2020

Jasmonic acid (JA) plays a crucial role in various biological processes including development, signal transduction and stress response. Allene oxide synthase (AOS) catalyzing (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT) to an unstable allene oxide is involved in the first step of JA biosynthesis. Here, we isolated the PtAOS1 gene and its promoter from trifoliate orange (Poncirus trifoliata). PtAOS1 contains a putative chloroplast targeting sequence in N-terminal and shows relative to pistachio (Pistacia vera) AOS. A number of stress-, light- and hormone-related cis-elements were found in the PtAOS1 promoter which may be responsible for the up-regulation of PtAOS1 under drought and JA treatments. Transient expression in tobacco (Nicotiana benthamiana) demonstrated that the P−532 (−532 to +1) fragment conferring drive activity was a core region in the PtAOS1 promoter. Using yeast one-hybrid, three novel proteins, PtDUF886, PtDUF1685 and PtRAP2.4, binding to P−532 were identified. The dual luciferase assay in tobacco illustrated that all three transcription factors could enhance PtAOS1 promoter activity. Genes PtDUF1685 and PtRAP2.4 shared an expression pattern which was induced significantly by drought stress. These findings should be available evidence for trifoliate orange responding to drought through JA modulation.
Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling
Authors: Qian Heying , Li Gang , Zhao Guodong , Liu Mingzhu , Xu Anying
Published: July 2020

Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm disease that is especially harmful to cocoon production and seriously restricts sericultural development. Our laboratory successfully cultivated a new highly BmNPV-resistant silkworm variety, Huakang 2; however, its mechanism of BmNPV resistance remains unclear. To understand its resistance mechanism, we conducted a metabolomic and transcriptomic study of the midgut of silkworm varieties, Baiyu N and Baiyu after BmNPV infection. We identified 451 differential metabolites, which were mostly comprised of small molecules, such as saccharides, acids, amines, alcohols, and glycosides. We found that the primary differences in disease resistance between the silkworm varieties are metabolic-pathways, tryptophan metabolism, oxidative phosphorylation, ABC-transporters, beta-alanine metabolism, and phenylalanine metabolism. Combined analysis with transcriptomic data suggested that tryptophan metabolism and oxidative phosphorylation are closely related to the silkworms’ BmNPV resistance. We hypothesize that the roles of the two metabolic pathways in the BmNPV resistance mechanism might be the following: Oxidative phosphorylation generates a large amount of adenosine triphosphate (ATP) in response to BmNPV infection to provide silkworms the energy required for establishing BmNPV resistance. Tryptophan metabolism then activates the aryl hydrocarbon receptor (AhR) through the exogenous virus BmNPV, which activates the silkworm’s immune system to defeat BmNPV infections.
Pharmacogenomics of Hypertension Treatment
Authors: Rysz Jacek , Franczyk Beata , Rysz-Górzyńska Magdalena , Gluba-Brzózka Anna
Published: July 2020

Hypertension is one of the strongest modifiable cardiovascular risk factors, affecting an increasing number of people worldwide. Apart from poor medication adherence, the low efficacy of some therapies could also be related to inter-individual genetic variability. Genetic studies of families revealed that heritability accounts for 30% to 50% of inter-individual variation in blood pressure (BP). Genetic factors not only affect blood pressure (BP) elevation but also contribute to inter-individual variability in response to antihypertensive treatment. This article reviews the recent pharmacogenomics literature concerning the key classes of antihypertensive drugs currently in use (i.e., diuretics, β-blockers, ACE inhibitors, ARB, and CCB). Due to the numerous studies on this topic and the sometimes-contradictory results within them, the presented data are limited to several selected SNPs that alter drug response. Genetic polymorphisms can influence drug responses through genes engaged in the pathogenesis of hypertension that are able to modify the effects of drugs, modifications in drug–gene mechanistic interactions, polymorphisms within drug-metabolizing enzymes, genes related to drug transporters, and genes participating in complex cascades and metabolic reactions. The results of numerous studies confirm that genotype-based antihypertension therapies are the most effective and may help to avoid the occurrence of major adverse events, as well as decrease the costs of treatment. However, the genetic heritability of drug response phenotypes seems to remain hidden in multigenic and multifactorial complex traits. Therefore, further studies are required to analyze all associations and formulate final genome-based treatment recommendations.
Microenvironment Molecular Profile Combining Glycation Adducts and Cytokines Patterns on Secretome of Short-term Blood-derived Cultures during Tumour Progression
Authors: Coluccio Maria Laura , Presta Ivan , Greco Marta , Gervasi Rita , La Torre Domenico , Renne Maria , Voci Carlo Pietro , Lunelli Lorenzo , Donato Giuseppe , Malara Natalia
Published: July 2020

Cancer cells are known to secrete many bioactive factors acting both with paracrine and autocrine mechanisms by which they condition the surrounding microenvironment. At the same time, the intracytoplasmic metabolic activities microenvironment influences the profile of this secretion. It is well known that cancer cells exhibit prevalent glycolytic metabolism and a more oxidative atmosphere compared to their healthy counterparts; this metabolic phenotype promotes glycate adducts formation and secretion. Considering the exacerbation of metabolic changes during the cancer progression, it is suggestive to explore the potential correlation between the increasing rate of glycan adducts and the specific pattern of secreted cytokines in different phases of cancer disease. We analyzed the secretomes of blood-derived cancer cell cultures from cancer patients and healthy subjects. The relative glycate adducts content in cancer secretomes was higher in comparison to that of healthy samples. Moreover, the stratification based on different phases of cancer disease correlated with a specific cytokines panel. The results obtained open a new perspective of observation of the intricate relationship between metabolome and inflammation in cancer. By using the analysis of secretome combined with a standardized protocol of liquid biopsy, it would be possible to identify specific profiles of molecular markers useful to arrange alternative and personalized medicine strategies.
The Bone’s Role in Myeloid Neoplasia
Authors: Kazianka Lukas , Staber Philipp B
Published: July 2020

The interaction of hematopoietic stem and progenitor cells with their direct neighboring cells in the bone marrow (the so called hematopoietic niche) evolves as a key principle for understanding physiological and malignant hematopoiesis. Significant progress in this matter has recently been achieved making use of emerging high-throughput techniques that allow characterization of the bone marrow microenvironment at single cell resolution. This review aims to discuss these single cell findings in the light of other conventional niche studies that together define the current notion of the niche’s implication in (i) normal hematopoiesis, (ii) myeloid neoplasms and (iii) disease-driving pathways that can be exploited to establish novel therapeutic strategies in the future.
Sequesterpene Lactones Isolated from a Brazilian Cerrado Plant (Eremanthus spp.) as Anti-Proliferative Compounds, Characterized by Functional and Proteomic Analysis, Are Candidates for New Therapeutics in Glioblastoma
Authors: Izumi Clarice , Laure Helen Julie , Barbosa Nayara Gonçalves , Hassibe Thomé Carolina , Aguiar Ferreira Germano , Paulo Barreto Sousa João , Peporine Lopes Norberto , César Rosa José
Published: July 2020

Gliomas are responsible for more than 60% of all primary brain tumors. Glioblastoma multiforme (GBM), a grade IV tumor (WHO), is one of the most frequent and malignant gliomas. Despite two decades of advances in the discovery of new markers for GBM, the chemotherapy of choice falls to temozolomide after surgery and radiotherapy, which are not enough to increase the survival of patients to more than 15 months. It is urgent to discover new anti-glioma compounds. Many compounds derived from natural products have been used in the development of anti-tumor drugs. In this work, we have screened six low molecular weight sesquiterpene lactones, isolated from Eremanthus spp., and studied their function as anti-proliferative agents against GBM strains. We demonstrated that two of them, goyazensolide and lychnofolide, were effective in reducing cell viability, preventing the formation of anchorage-dependent colony and were able to pass through a mimetic blood-brain barrier making them candidates for glioma therapy, being more potent than temozolomide, according to in vitro assays for the cell lines tested. Proteomic analysis revealed a number of altered proteins involved in glycolytic metabolism and cellular catabolism.
Inflammasome and Mitophagy Connection in Health and Disease
Authors: Yuk Jae-Min , Silwal Prashanta , Jo Eun-Kyeong
Published: July 2020

The inflammasome is a large intracellular protein complex that activates inflammatory caspase-1 and induces the maturation of interleukin (IL)-1β and IL-18. Mitophagy plays an essential role in the maintenance of mitochondrial homeostasis during stress. Previous studies have indicated compelling evidence of the crosstalk between inflammasome and mitophagy. Mitophagy regulation of the inflammasome, or vice versa, is crucial for various biological functions, such as controlling inflammation and metabolism, immune and anti-tumor responses, and pyroptotic cell death. Uncontrolled regulation of the inflammasome often results in pathological inflammation and pyroptosis, and causes a variety of human diseases, including metabolic and inflammatory diseases, infection, and cancer. Here, we discuss how improved understanding of the interactions between inflammasome and mitophagy can lead to novel therapies against various disease pathologies, and how the inflammasome-mitophagy connection is currently being targeted pharmacologically by diverse agents and small molecules. A deeper understanding of the inflammasome-mitophagy connection will provide new insights into human health and disease through the balance between mitochondrial clearance and pathology.
Mechanisms that Link Chronological Aging to Cellular Quiescence in Budding Yeast
Authors: Mohammad Karamat , Baratang Junio Jennifer Anne , Tafakori Tala , Orfanos Emmanuel , Titorenko Vladimir I.
Published: July 2020

After Saccharomyces cerevisiae cells cultured in a medium with glucose consume glucose, the sub-populations of quiescent and non-quiescent cells develop in the budding yeast culture. An age-related chronology of quiescent and non-quiescent yeast cells within this culture is discussed here. We also describe various hallmarks of quiescent and non-quiescent yeast cells. A complex aging-associated program underlies cellular quiescence in budding yeast. This quiescence program includes a cascade of consecutive cellular events orchestrated by an intricate signaling network. We examine here how caloric restriction, a low-calorie diet that extends lifespan and healthspan in yeast and other eukaryotes, influences the cellular quiescence program in S. cerevisiae. One of the main objectives of this review is to stimulate an exploration of the mechanisms that link cellular quiescence to chronological aging of budding yeast. Yeast chronological aging is defined by the length of time during which a yeast cell remains viable after its growth and division are arrested, and it becomes quiescent. We propose a hypothesis on how caloric restriction can slow chronological aging of S. cerevisiae by altering the chronology and properties of quiescent cells. Our hypothesis posits that caloric restriction delays yeast chronological aging by targeting four different processes within quiescent cells.
Roads Detection and Parametrization in Integrated BIM-GIS Using LiDAR
Authors: Barazzetti Luigi , Previtali Mattia , Scaioni Marco
Published: July 2020

Building Information Modeling (BIM) has a crucial role in smart road applications, not only limited to the design and construction stages, but also to traffic monitoring, autonomous vehicle navigation, road condition assessment, and real-time data delivery to drivers, among others. Point clouds collected through LiDAR are a powerful solution to capture as-built conditions, notwithstanding the lack of commercial tools able to automatically reconstruct road geometry in a BIM environment. This paper illustrates a two-step procedure in which roads are automatically detected and classified, providing GIS layers with basic road geometry that are turned into parametric BIM objects. The proposed system is an integrated BIM-GIS with a structure based on multiple proposals, in which a single project file can handle different versions of the model using a variable level of detail. The model is also refined by adding parametric elements for buildings and vegetation. Input data for the integrated BIM-GIS can also be existing cartographic layers or outputs generated with algorithms able to handle LiDAR data. This makes the generation of the BIM-GIS more flexible and not limited to the use of specific algorithms for point cloud processing.