bluuebunny/crossref_metadata_2025 huggingface dataset:
Theoretical Analysis of Entropy Generation at the Blade Interface of a Tubular Turbine Under Cooperative Conditions
Authors: Li Zhenggui , Cheng Chuang , Yan Shengnan , Peng Shengyang , Ma Biao
Published: December 2021

To study the influence of the blade entropy production range on the efficiency of a tubular turbine under coassociated conditions, the renormalization group K–ε turbulence model was used to simulate the full flow passage of the tubular turbine based on the Navier–Stokes equation, and the blade interface was analyzed using the eddy analysis method and entropy production theory. The results reveal that there is a strong correlation between the size of the high-entropy production area and the level of association. If the level of association is high, the size of the high-entropy production is small, and the turbine efficiency is high. Furthermore, if the level of association is low, the size of the high-entropy production area is large, and the turbine efficiency is low. Under small opening and small flow conditions, the blade entropy generation is due to the sharp change in the velocity gradient caused by the vortex on the blade. Under large opening and large flow conditions, the blade entropy production is mainly due to the friction loss caused by the impact of high-speed water flow.
Simultaneous Determination of Active Clinical Components of Teicoplanin and Ramoplanin in Environmental Water by LC-MS/MS Coupled With Cascade Elution
Authors: Jin Hui , Zhao Cheng , Yin Yi , Zheng Guangming , li Lichun , Shan Qi , Zhang Meiyu , Wei Linting , Shi Xiaona , Huang Heqing , Zhang Wenjing , Liu Shugui
Published: December 2021

A simple, sensitive, and simultaneous method was established and validated for the active clinical components of teicoplanin and ramoplanin in environmental water by LC-MS/MS coupled with cascade elution. Moreover, a cascade elution method, which was rapid, solvent-less, and high-extraction efficient was successfully proposed to realize the extraction and purification of seven targets in one step. Under optimized conditions, the method showed excellent linearity with the correlation coefficient (R2) ≥0.998 in the range of 1.0–100.0 ng L−1. Low matrix effects and good recoveries which ranged from 86 to 114% were reached with RSDs lower than 3.0% for most targets. The limits of detection and limit of quantification were 0.1–1.3 and 0.3–4.0 ng L−1, respectively. This method was successfully applied for the determination of teicoplanin and ramoplanin in water samples from the Pearl River and the South China Sea. TA2-2,3 was quantified in only one sample with the concentration of 8.0 ng L−1.
Lessons Learned From Translational Research in Neuromuscular Diseases: Impact on Study Design, Outcome Measures and Managing Expectation
Authors: Stimpson Georgia , Chesshyre Mary , Baranello Giovanni , Muntoni Francesco
Published: December 2021

Spinal Muscular Atrophy (SMA) and Duchenne Muscular Dystrophy (DMD), two of the most common, child onset, rare neuromuscular disorders, present a case study for the translation of preclinical research into clinical work. Over the past decade, well-designed clinical trials and innovative methods have led to the approval of several novel therapies for SMA and DMD, with many more in the pipeline. This review discusses several features that must be considered during trial design for neuromuscular diseases, as well as other rare diseases, to maximise the possibility of trial success using historic examples. These features include well-defined inclusion criteria, matching criteria, alternatives to placebo-controlled trials and the selection of trial endpoints. These features will be particularly important in the coming years as the investigation into innovative therapy approaches for neuromuscular diseases continues.
Flagellin From Pseudomonas aeruginosa Modulates SARS-CoV-2 Infectivity in Cystic Fibrosis Airway Epithelial Cells by Increasing TMPRSS2 Expression
Authors: Ruffin Manon , Bigot Jeanne , Calmel Claire , Mercier Julia , Givelet Maëlle , Oliva Justine , Pizzorno Andrés , Rosa-Calatrava Manuel , Corvol Harriet , Balloy Viviane , Terrier Olivier , Guillot Loïc
Published: December 2021

In the coronavirus disease 2019 (COVID-19) health crisis, one major challenge is to identify the susceptibility factors of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in order to adapt the recommendations for populations, as well as to reduce the risk of COVID-19 development in the most vulnerable people, especially patients with chronic respiratory diseases such as cystic fibrosis (CF). Airway epithelial cells (AECs) play a critical role in the modulation of both immune responses and COVID-19 severity. SARS-CoV-2 infects the airway through the receptor angiotensin-converting enzyme 2, and a host protease, transmembrane serine protease 2 (TMPRSS2), plays a major role in SARS-CoV-2 infectivity. Here, we show that Pseudomonas aeruginosa increases TMPRSS2 expression, notably in primary AECs with deficiency of the ion channel CF transmembrane conductance regulator (CFTR). Further, we show that the main component of P. aeruginosa flagella, the protein flagellin, increases TMPRSS2 expression in primary AECs and Calu-3 cells, through activation of Toll-like receptor-5 and p38 MAPK. This increase is particularly seen in Calu-3 cells deficient for CFTR and is associated with an intracellular increased level of SARS-CoV-2 infection, however, with no effect on the amount of virus particles released. Considering the urgency of the COVID-19 health crisis, this result may be of clinical significance for CF patients, who are frequently infected with and colonized by P. aeruginosa during the course of CF and might develop COVID-19.
Dissecting the Landscape of Activated CMV-Stimulated CD4+ T Cells in Humans by Linking Single-Cell RNA-Seq With T-Cell Receptor Sequencing
Authors: Lyu Menghua , Wang Shiyu , Gao Kai , Wang Longlong , Zhu Xijun , Liu Ya , Wang Meiniang , Liu Xiao , Li Bin , Tian Lei
Published: December 2021

CD4+ T cells are crucial in cytomegalovirus (CMV) infection, but their role in infection remains unclear. The heterogeneity and potential functions of CMVpp65-reactivated CD4+ T cell subsets isolated from human peripheral blood, as well as their potential interactions, were analyzed by single-cell RNA-seq and T cell receptor (TCR) sequencing. Tregs comprised the largest population of these reactivated cells, and analysis of Treg gene expression showed transcripts associated with both inflammatory and inhibitory functions. The detailed phenotypes of CMV-reactivated CD4+ cytotoxic T1 (CD4+ CTL1), CD4+ cytotoxic T2 (CD4+ CTL2), and recently activated CD4+ T (Tra) cells were analyzed in single cells. Assessment of the TCR repertoire of CMV-reactivated CD4+ T cells confirmed the clonal expansion of stimulated CD4+ CTL1 and CD4+ CTL2 cells, which share a large number of TCR repertoires. This study provides clues for resolving the functions of CD4+ T cell subsets and their interactions during CMV infection. The specific cell groups defined in this study can provide resources for understanding T cell responses to CMV infection.
IL-23 Enhances C-Fiber-Mediated and Blue Light-Induced Spontaneous Pain in Female Mice
Authors: Ji Jasmine , He Qianru , Luo Xin , Bang Sangsu , Matsuoka Yutaka , McGinnis Aidan , Nackley Andrea G. , Ji Ru-Rong
Published: December 2021

The incidence of chronic pain is especially high in women, but the underlying mechanisms remain poorly understood. Interleukin-23 (IL-23) is a pro-inflammatory cytokine and contributes to inflammatory diseases (e.g., arthritis and psoriasis) through dendritic/T cell signaling. Here we examined the IL-23 involvement in sexual dimorphism of pain, using an optogenetic approach in transgenic mice expressing channelrhodopsin-2 (ChR2) in TRPV1-positive nociceptive neurons. In situ hybridization revealed that compared to males, females had a significantly larger portion of small-sized (100-200 μm2) Trpv1+ neurons in dorsal root ganglion (DRG). Blue light stimulation of a hindpaw of transgenic mice induced intensity-dependent spontaneous pain. At the highest intensity, females showed more intense spontaneous pain than males. Intraplantar injection of IL-23 (100 ng) induced mechanical allodynia in females only but had no effects on paw edema. Furthermore, intraplantar IL-23 only potentiated blue light-induced pain in females, and intrathecal injection of IL-23 also potentiated low-dose capsaicin (500 ng) induced spontaneous pain in females but not males. IL-23 expresses in DRG macrophages of both sexes. Intrathecal injection of IL-23 induced significantly greater p38 phosphorylation (p-p38), a marker of nociceptor activation, in DRGs of female mice than male mice. In THP-1 human macrophages estrogen and chemotherapy co-application increased IL-23 secretion, and furthermore, estrogen and IL-23 co-application, but not estrogen and IL-23 alone, significantly increased IL-17A release. These findings suggest a novel role of IL-23 in macrophage signaling and female-dominant pain, including C-fiber-mediated spontaneous pain. Our study has also provided new insight into cytokine-mediated macrophage-nociceptor interactions, in a sex-dependent manner.
Risk for Cardiovascular Adverse Events Associated With Sphingosine-1-Phosphate Receptor Modulators in Patients With Multiple Sclerosis: Insights From a Pooled Analysis of 15 Randomised Controlled Trials
Authors: Zhao Zhao , Lv Yang , Gu Zhi-Chun , Ma Chun-Lai , Zhong Ming-Kang
Published: December 2021

Background

All agents engaging sphongosine-1-phospate receptors (S1PRs) will have some cardiovascular effect. This study aimed to elucidate the risk of cardiovascular adverse events (AEs) in patients with multiple sclerosis (MS) treated with S1PR modulators (S1PRMs).

Methods

We systematically searched the PubMed, EMBASE, and Cochrane Library databases for randomised controlled trials (RCTs) published through January 5, 2021. Relative risks (RRs) and 95% confidence intervals (CIs) were calculated using the random-effects model. Sensitivity analyses and meta-regression were performed.

Results

Seventeen RCTs (12 for fingolimod; 3 for ozanimod; 2 for siponimod) involving 13,295 patients were included. Compared with the control treatment, S1PRMs significantly increased the risk of cardiovascular AEs (RR, 2.21; 95% CI, 1.58–3.10; I2, 75.6%). Notably, the high-risk cardiovascular AEs associated with S1PRMs were primarily bradyarrhythmia (RR, 2.92; 95% CI, 1.91–4.46; I2, 30.8%) and hypertension (RR, 2.00; 95% CI, 1.49–2.67; I2, 56.5%). Subgroup analysis results were consistent with the primary outcomes except that ozanimod was associated with a higher risk of hypertension only (RR, 1.76; 95% CI, 1.10–2.82; I2, 0.0%), while siponimod was associated with a higher risk of bradyarrhythmia only (RR, 2.75; 95% CI, 1.75–4.31; I2, 0.0%). No significant inter-subgroup differences were observed (Pinteraction > 0.05).

Conclusions

S1PRM use increased the risk of cardiovascular AEs by 1.21 times in patients with MS, and increased risks for bradyarrhythmia and hypertension were at 2.92- and 2.00-fold, respectively. These findings can help clinicians assess the risk of cardiovascular AEs in patients treated with S1PRMs.

Systematic Review Registration

The PROSPERO ID is CRD42020183215.
Distribution of Polychlorinated Naphthalenes in Sediment From Industrialized Coastal Waters of Korea With the Optimized Cleanup and GC-MS/MS Methods
Authors: Lee Ha-Hyun , Lee Sunggyu , Lee Jung Suk , Moon Hyo-Bang
Published: December 2021

Limited studies have been conducted on polychlorinated naphthalenes (PCNs) in the coastal environment worldwide. In this study, analytical methods were optimized for 18 PCN congeners in sediment using a multi-layer silica gel column and a gas chromatograph coupled to tandem mass spectrometry (GC-MS/MS). The optimized analytical methods of PCNs were employed for sediment samples from heavily industrialized bays of Korea to assess the occurrence, contamination, potential sources, and ecotoxicological concerns. PCNs were detected in all sediment samples, indicating ubiquitous contamination in industrialized coastal regions of Korea. Total concentrations and toxic equivalents (TEQs) of PCNs ranged from 0.99 to 21,500 (mean: 568) pg/g dry weight and from 1.72 × 10–5 to 18.8 (mean: 0.52) pg TEQ/g dry weight, respectively, which were within the ranges reported by other studies. A clear decreasing gradient was observed for the sedimentary PCNs from inner to outer parts of the bays, streams, and rivers. This result indicates that industrial activities are primary sources of PCNs. The highest PCN concentrations were observed in sediment close to non-ferrous and petrochemical industries, indicating potential sources. CNs 73 and 52 were predominant congeners of PCNs in all sediment samples. Diagnostic ratios and non-parametric multidimensional scaling analysis showed that the potential primary sources of PCNs are thermal-related emissions and the use of PCB technical mixtures. Although a few sediment samples exceeded the sediment quality guidelines of TEQs, the cumulative risks by dioxin-like contaminants may be caused for almost all coastal zones surveyed. This is the first report on PCNs in sediment from Korean coastal waters.
Norepinephrine Dosage Is Associated With Lactate Clearance After Resuscitation in Patients With Septic Shock
Authors: Yu Chao , Fan Wenjing , Shao Min
Published: December 2021

Background: Some septic shock patients have persistent hyperlactacidemia despite a normal systemic hemodynamics after resuscitation. Central venous oxygen saturation (ScvO2), mean arterial pressure (MAP), and central venous pressure (CVP) cannot be target in subsequent hemodynamic treatments. Vasoplegia is considered to be one of the main causes of oxygen metabolism abnormalities in septic shock patients, and norepinephrine (NE) is the first-line vasopressor in septic shock treatment; its dosage represents the severity of vasoplegia. This study was performed to determine whether vasoplegia, as assessed by NE dosage, can indicate patients' lactate clearance after the completion of resuscitation.

Methods: A retrospective study was performed, and 106 patients with septic shock in an intensive care unit were analyzed. Laboratory values and hemodynamic variables were obtained upon completion of resuscitation (H 0) and 6 h after (H 6). Lactate clearance was defined as the percent decrease in lactate from H 0 to H 6. Student's t-test, Mann-Whitney U-test, Chi-square or Fisher's exact tests, logistic regression analysis, and receiver operating characteristic (ROC) curve analysis were performed for statistical analysis.

Results: Patients with a mean age of 63.7 ± 13.8 years, baseline APACHE II score of 21.0 ± 5.1, and SOFA score of 12.7 ± 2.7 were enrolled. The study found that after 6-h of resuscitation, lactate clearance (LC) was <10% in 33 patients (31.1%). Patients with 6-h LC <10% compared with 6-h LC ≥ 10% had a higher NE dose (μg·kg−1·min−1) (0.55 [0.36–0.84] vs. 0.25 [0.18–0.41], p < 0.001). Multivariate logistic regression analysis of statistically significant univariate variables showed that NE dose had a significant inverse relationship with 6-h LC < 10%. The cutoff for NE was ≥ 0.32 μg·kg−1·min−1 for predicting 6-h lactate clearance after resuscitation, with a sensitivity of 75.76% and a specificity of 70.00%. Septic shock patients with an NE dose ≥ 0.32 μg·kg−1·min−1, relative to patients with an NE dose < 0.32 μg·kg−1·min−1, had a greater 30-day mortality rate (69.8% vs. 26.4% p < 0.001).

Conclusion: Some patients with septic shock had persistent oxygen metabolism disorders after hemodynamic resuscitation. NE dose may indicate vasoplegia and oxygen metabolism disorder. After resuscitation, septic shock patients with high-dose NE have lower lactate clearance and a greater 30-day mortality rate than those with low-dose NE.
A Systematic, Open-Science Framework for Quantification of Cell-Types in Mouse Brain Sections Using Fluorescence Microscopy
Authors: Sanchez-Arias Juan C. , Carrier Micaël , Frederiksen Simona D. , Shevtsova Olga , McKee Chloe , van der Slagt Emma , Gonçalves de Andrade Elisa , Nguyen Hai Lam , Young Penelope A. , Tremblay Marie-Ève , Swayne Leigh Anne
Published: December 2021

The ever-expanding availability and evolution of microscopy tools has enabled ground-breaking discoveries in neurobiology, particularly with respect to the analysis of cell-type density and distribution. Widespread implementation of many of the elegant image processing tools available continues to be impeded by the lack of complete workflows that span from experimental design, labeling techniques, and analysis workflows, to statistical methods and data presentation. Additionally, it is important to consider open science principles (e.g., open-source software and tools, user-friendliness, simplicity, and accessibility). In the present methodological article, we provide a compendium of resources and a FIJI-ImageJ-based workflow aimed at improving the quantification of cell density in mouse brain samples using semi-automated open-science-based methods. Our proposed framework spans from principles and best practices of experimental design, histological and immunofluorescence staining, and microscopy imaging to recommendations for statistical analysis and data presentation. To validate our approach, we quantified neuronal density in the mouse barrel cortex using antibodies against pan-neuronal and interneuron markers. This framework is intended to be simple and yet flexible, such that it can be adapted to suit distinct project needs. The guidelines, tips, and proposed methodology outlined here, will support researchers of wide-ranging experience levels and areas of focus in neuroscience research.
Characterization of Enterovirus Associated m6A RNA Methylation in Children With Neurological Symptoms: A Prospective Cohort Study
Authors: Zhu Danping , Song Yongling , Hu Dandan , Li Suyun , Liu Guangming , Li Peiqing , Yang Sida
Published: December 2021

Little is known about the particular changes of N6-methyladenosine (m6A) RNA methylation in enterovirus (EV) infection among children with neurologic symptoms. Here, we determined the characterization of EV associated m6A RNA methylation in this population. A prospective cohort study was conducted from 2018/2 to 2019/12 at the Guangzhou Women and Children’s Medical Center. We included EV infected children with and without neurological symptoms. High-throughput m(6)A-RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-seq analysis were used to evaluate the m6A RNA methylation and transcript expression of cerebrospinal fluid samples. The functional annotation and pathways of differentially methylated m6A genes with synchronously differential expression were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Seven patients were enrolled in the control group, and 13 cases were in the neurological symptoms (NS) group. A total of 3472 differentially expressed genes and 957 m6A modified genes were identified. A conjoint analysis of MeRIP-seq and RNA-seq data found 1064 genes with significant changes in both the m6A modifications and mRNA levels. The different m6A RNA methylation was increased in the transcriptome’s CDS regions but decreased in both the 3′UTRs and stop codon among the NS group. Functional annotation like the “oxidative phosphorylation” gene pathway, “Parkinson’s disease” and GO terms like “respiratory electron transport chain,” “cellular metabolic process,” and “oxidation-reduction process” was enriched in symptomatic patients. Our study elucidated the changes of RNA m6A methylation patterns and related cellular functions and signaling pathways in EV patients with neurologic symptoms.
Whole Grain Qingke Attenuates High-Fat Diet-Induced Obesity in Mice With Alterations in Gut Microbiota and Metabolite Profile
Authors: Li Xipu , Suo Jingqi , Huang Xinguo , Dai Huifen , Bian Hongwu , Zhu Muyuan , Lin Weiqiang , Han Ning
Published: December 2021

Whole grain Qingke (WGQK) displays anti-obesity and lipid-lowering properties; however, the underlying mechanism remains elusive. This study investigated the alteration of gut microbiota composition and metabolite profile induced by WGQK intervention in mice through the integration of 16S ribosomal RNA (rRNA) sequencing and an untargeted metabolomics study. C57BL/6J male mice were fed a normal control diet (NC), high-fat diet (HFD), and HFD plus 30% WGQK (HFD+QK) for 16 weeks. The WGQK intervention decreased body weight gain, glucose tolerance, and serum lipid levels, and alleviated liver function damage induced by HFD. Moreover, WGQK changed gut microbiota composition and enriched specific genera such as Akkermansia, Bifidobacterium, and Lactobacillus. Fecal metabolomics analysis indicated that WGQK enhanced the abundance of tryptophan metabolism-related metabolites (indole, 3-indoleacetic acid, indole acetic acid (IAA), 5-hydroxyindole-3-acetic acid), histidine metabolism-related metabolites (histamine), and some unsaturated fatty acids (oleic acid, 9,10-dihydroxy-12Z-octadecenoic acid, and alpha-linolenic acid). Spearman correlation analysis revealed that these metabolites were negatively correlated with obesity-related parameters and positively correlated with the gut genera enriched by WGQK. Moreover, WGQK promoted the expression of Cholesterol 7α-hydroxylase (CYP7A1) responsible for primary bile acids production, accompanied by a decline in intestinal FXR-FGF15 expression levels. The transcript levels of two genes associated with lipogenesis, such as lipid fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) were also decreased in the HFD+QK group. Overall, our results suggest interactions between gut microbial shifts and host amino acid/lipid metabolism, and shed light on the mechanisms underlying the anti-obesity effect of WGQK.
Survival Advantage Following TAG-72 Antigen-Directed Cancer Surgery in Patients With Colorectal Carcinoma: Proposed Mechanisms of Action
Authors: Hitchcock Charles L. , Povoski Stephen P. , Mojzisik Cathy M. , Martin Edward W.
Published: December 2021

Patients with colorectal carcinoma (CRC) continue to have variable clinical outcomes despite undergoing the same surgical procedure with curative intent and having the same pathologic and clinical stage. This problem suggests the need for better techniques to assess the extent of disease during surgery. We began to address this problem 35 years ago by injecting patients with either primary or recurrent CRC with 125I-labeled murine monoclonal antibodies against the tumor-associated glycoprotein-72 (TAG-72) and using a handheld gamma-detecting probe (HGDP) for intraoperative detection and removal of radioactive, i.e., TAG-72-positive, tissue. Data from these studies demonstrated a significant difference in overall survival data (p < 0.005 or better) when no TAG-72-positive tissue remained compared to when TAG-72-positive tissue remained at the completion of surgery. Recent publications indicate that aberrant glycosylation of mucins and their critical role in suppressing tumor-associated immune response help to explain the cellular mechanisms underlying our results. We propose that monoclonal antibodies to TAG-72 recognize and bind to antigenic epitopes on mucins that suppress the tumor-associated immune response in both the tumor and tumor-draining lymph nodes. Complete surgical removal of all TAG-72-positive tissue serves to reverse the escape phase of immunoediting, allowing a resetting of this response that leads to improved overall survival of the patients with either primary or recurrent CRC. Thus, the status of TAG-72 positivity after resection has a significant impact on patient survival.
Development and Validation of a Scoring System for Early Diagnosis of Malignant Pleural Effusion Based on a Nomogram
Authors: Wu Aihua , Liang Zhigang , Yuan Songbo , Wang Shanshan , Peng Weidong , Mo Yijun , Yang Jing , Liu Yanqing
Published: December 2021

Background

The diagnostic value of clinical and laboratory features to differentiate between malignant pleural effusion (MPE) and benign pleural effusion (BPE) has not yet been established.

Objectives

The present study aimed to develop and validate the diagnostic accuracy of a scoring system based on a nomogram to distinguish MPE from BPE.

Methods

A total of 1,239 eligible patients with PE were recruited in this study and randomly divided into a training set and an internal validation set at a ratio of 7:3. Logistic regression analysis was performed in the training set, and a nomogram was developed using selected predictors. The diagnostic accuracy of an innovative scoring system based on the nomogram was established and validated in the training, internal validation, and external validation sets (n = 217). The discriminatory power and the calibration and clinical values of the prediction model were evaluated.

Results

Seven variables [effusion carcinoembryonic antigen (CEA), effusion adenosine deaminase (ADA), erythrocyte sedimentation rate (ESR), PE/serum CEA ratio (CEA ratio), effusion carbohydrate antigen 19-9 (CA19-9), effusion cytokeratin 19 fragment (CYFRA 21-1), and serum lactate dehydrogenase (LDH)/effusion ADA ratio (cancer ratio, CR)] were validated and used to develop a nomogram. The prediction model showed both good discrimination and calibration capabilities for all sets. A scoring system was established based on the nomogram scores to distinguish MPE from BPE. The scoring system showed favorable diagnostic performance in the training set [area under the curve (AUC) = 0.955, 95% confidence interval (CI) = 0.942–0.968], the internal validation set (AUC = 0.952, 95% CI = 0.932–0.973), and the external validation set (AUC = 0.973, 95% CI = 0.956–0.990). In addition, the scoring system achieved satisfactory discriminative abilities at separating lung cancer-associated MPE from tuberculous pleurisy effusion (TPE) in the combined training and validation sets.

Conclusions

The present study developed and validated a scoring system based on seven parameters. The scoring system exhibited a reliable diagnostic performance in distinguishing MPE from BPE and might guide clinical decision-making.
A Dual Role for Abscisic Acid Integrating the Cold Stress Response at the Whole-Plant Level in Iris pseudacorus L. Growing in a Natural Wetland
Authors: Caselles Vicent , Casadesús Andrea , Munné-Bosch Sergi
Published: December 2021

Leaf senescence, the last stage of the developmental program of leaves, can be induced by both internal and external signals. Cold stress-induced leaf senescence is an efficient strategy to overcome winter temperatures. In this work, we studied leaf senescence in yellow flag (Iris pseudacorus L.) individuals growing in a natural wetland, not only considering its relationship with external and internal cues, but also the plant developmental program, and the biological significance of rhizomes, storage organs that remain viable through winter. Total chlorophyll contents and the maximum efficiency of PSII (Fv/Fm ratio) decreased in senescing leaves, which was associated with a sharp increase in abscisic acid (ABA) contents. Furthermore, total cytokinin and 2-isopentenyladenine contents decreased in December compared to November, as plants became more stressed due to a decline in air temperatures. ABA increases in senescing leaves increased in parallel to reductions in violaxanthin. Rhizomes also accumulated large amounts of ABA during winter, while roots did not, and neither roots nor rhizomes accumulated 9-cis-epoxycarotenoids, thus suggesting ABA, which might play a role in conferring cold tolerance to this subterranean organ, may result from phloem transport from senescing leaves. It is concluded that (i) leaf senescence is a highly regulated physiological process in yellow flag playing a key role in the modulation of the entire plant developmental program, and (ii) ABA plays a major role not only in the regulation of leaf senescence but also in the establishment of cold tolerance in rhizomes, two processes that appear to be intimately interconnected.
Internet Use and Depressive Symptoms Among Older Adults in China
Authors: Yang Hua-lei , Zhang Shuo , Zhang Si-qing , Xie Lin , Wu Yuan-yang , Yao Yi-dan , Tang Li-li , Li Zhi-yun
Published: December 2021

This study investigated the effect of using the Internet on depression symptoms of older Chinese, based on 7,801 adults aged over 60 years from the 2018 China Family Panel Studies. Results showed that the elderly who used the Internet reported lower depression scores, and the more frequent they use Internet, the lower their depression scores. Moreover, using the Internet for social contact and entertainment decreased the depression scores of the older adults, but when using Internet for learn, work, and commercial activity, the relief of depressive symptoms disappeared. Therelief of depression symptoms through Internet use were heterogeneous among different groups: the elderly aged 60–70, women, rural residents, and those with lower education attainment. Moreover, Internet use decreased the depression scores by increasing the frequency of contact with their children and increasing the importance of their enjoyment of life. According to the relief of depression by using Internet reasonably, policies should be designed to ensure that all ages could have easy access to the Internet.
Association Between Household Income and Self-Perceived Health Status and Poor Mental and Physical Health Among Cancer Survivors
Authors: Su L. Joseph , O'Connor Sarah N. , Chiang Tung-Chin
Published: December 2021

Background: Health-related quality of life (HRQoL) is multidimensional and is composed of, at a minimum, self-perceived health status, physical functioning, and psychological well-being. HRQoL measures reflect the extent of disability and dysfunction associated with a chronic disease such as cancer. The objective of this study is to examine factors associated with HRQoL among cancer survivors.

Methods: Data from the 2009 Behavioral Risk Factor Surveillance System survey was used to examine factors associated with HRQoL among participants who reported having ever been diagnosed with cancer. Four questions associated with HRQoL included self-perceived health status, number of bad physical health days, and number of bad mental health days per month. Least square regression and logistic regression models, adjusted for confounding variables, were used for an ordinal and dichotomous [5 (bad) vs. 1–4 (excellent, very good, good, fair)] scale of HRQoL, respectively.

Results: Fifty nine thousand one hundred seventy three participants reported having ever been diagnosed with cancer. Adjusted mean self-perceived health status (5-point scale) among survivors of thyroid, colon, lung, cervical, breast, prostate, and ovarian cancer was 3.83 (0.05), 4.02 (0.04), 4.36 (0.06), 3.77 (0.03), 3.88 (0.03), 3.78 (0.04), and 3.96 (0.05), respectively. After adjusting for confounders, a positive dose-response effect was observed between income range and all three HRQoL measures across all seven cancer sites. Income was consistently and inversely associated with a higher chance for reporting poorer HRQoL [OR: 0.64, 95% CI: 0.57–0.71], [OR: 0.63, 95% CI: 0.48–0.82], [OR: 0.67, 95% CI: 0.56–0.80], [OR: 0.69, 95% CI: 0.56–0.86], [OR: 0.55, 95% CI: 0.49–0.62], [OR:0.55, 95% CI: 0.44–0.69], [OR: 0.75, 95% CI: 0.62–0.91] among those with thyroid, colon, lung, cervical, breast, prostate, and ovarian cancer, respectively.

Discussion: This study found that income range was associated with HRQoL among cancer survivors. It is plausible that financial resources may lessen the overall burden of cancer survivors, which could improve health-related quality of life among cancer survivors.
Relationship Between Polycyclic Aromatic Hydrocarbons and Cardiovascular Diseases: A Systematic Review
Authors: Mallah Manthar Ali , Mallah Mukhtiar Ali , Liu Yang , Xi He , Wang Wei , Feng Feifei , Zhang Qiao
Published: December 2021

Objective: The primary aim of this systematic review was to examine the relationship of polycyclic aromatic hydrocarbon (PAH) exposure with cardiovascular diseases (CVDs) and elaborate the current knowledge and recent advances in the area of PAH and its effects on CVDs and discuss the growing epidemiological evidence linking PAH to CVDs on the health of human populations. In this systematic review, the increased risk of cardiovascular diseases and their relationship with PAHs were discussed in detail.

Methods: On 05th April 2021, a systematic literature search was conducted using PubMed/Medline and Web of Science search engines in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. The search was limited to articles that were written in English and dealt with human issues. All original peer-review publications were considered for inclusion. Comments, case reports, reviews, duplicated papers, and conference reports were excluded. Data was collected from included papers by two independent reviewers.

Results: Conclusively, 20 research articles published between 2005 and 2021 were chosen for the final analysis. The systemic review included 20 studies with a variety of geographical studies. The most common research category among the nominated studies were time-series studies followed by retrospective cohort, cross-sectional, quasi-experimental, panel, and case-control studies. Most of the studies were conducted in the United States, whereas others were showed in various geographical countries around the world, such as Denmark, Germany, Finland, Netherlands, France, China, Norway, Korea, Sweden, Saudi Arabia, and Belgium. Eight studies assessed the association between PAH exposure and CVDs, four articles observed this relationship with blood pressure (BP), two observed association between atherosclerotic CVD and PAH, one congenital heart disease, cardiovascular events, and two with obesity. Furthermore, in some investigations, a favorable association between PAH exposure and hypertension as well as PAH exposure and obesity was found.

Conclusion: In conclusion, this systematic review examined the relationship of PAH exposure with CVDs and CVD-related risk factors by searching several digital databases. After a comprehensive literature searches and summarizing findings from 20 articles, the authors concluded that a positive relationship was observed between PAH exposure and CVD risks.
Benefits of Adaptive Learning Transfer From Typing-Based Learning to Speech-Based Learning
Authors: Wilschut Thomas , Sense Florian , van der Velde Maarten , Fountas Zafeirios , Maaß Sarah C. , van Rijn Hedderik
Published: December 2021

Memorising vocabulary is an important aspect of formal foreign-language learning. Advances in cognitive psychology have led to the development of adaptive learning systems that make vocabulary learning more efficient. One way these computer-based systems optimize learning is by measuring learning performance in real time to create optimal repetition schedules for individual learners. While such adaptive learning systems have been successfully applied to word learning using keyboard-based input, they have thus far seen little application in word learning where spoken instead of typed input is used. Here we present a framework for speech-based word learning using an adaptive model that was developed for and tested with typing-based word learning. We show that typing- and speech-based learning result in similar behavioral patterns that can be used to reliably estimate individual memory processes. We extend earlier findings demonstrating that a response-time based adaptive learning approach outperforms an accuracy-based, Leitner flashcard approach in learning efficiency (demonstrated by higher average accuracy and lower response times after a learning session). In short, we show that adaptive learning benefits transfer from typing-based learning, to speech based learning. Our work provides a basis for the development of language learning applications that use real-time pronunciation assessment software to score the accuracy of the learner’s pronunciations. We discuss the implications for our approach for the development of educationally relevant, adaptive speech-based learning applications.
Aerial Flight Paths for Communication
Authors: Bevins Alisha , Duncan Brittany A.
Published: December 2021

This article presents an understanding of naive users’ perception of the communicative nature of unmanned aerial vehicle (UAV) motions refined through an iterative series of studies. This includes both what people believe the UAV is trying to communicate, and how they expect to respond through physical action or emotional response. Previous work in this area prioritized gestures from participants to the vehicle or augmenting the vehicle with additional communication modalities, rather than communicating without clear definitions of the states attempting to be conveyed. In an attempt to elicit more concrete states and better understand specific motion perception, this work includes multiple iterations of state creation, flight path refinement, and label assignment. The lessons learned in this work will be applicable broadly to those interested in defining flight paths, and within the human-robot interaction community as a whole, as it provides a base for those seeking to communicate using non-anthropomorphic robots. We found that the Negative Attitudes towards Robots Scale (NARS) can be an indicator of how a person is likely to react to a UAV, the emotional content they are likely to perceive from a message being conveyed, and it is an indicator for the personality characteristics they are likely to project upon the UAV. We also see that people commonly associate motions from other non-verbal communication situations onto UAVs. Flight specific recommendations are to use a dynamic retreating motion from a person to encourage following, use a perpendicular motion to their field of view for blocking, simple descending motion for landing, and to use either no motion or large altitude changes to encourage watching. Overall, this research explores the communication from the UAV to the bystander through its motion, to see how people respond physically and emotionally.
Disorder at the Start: The Contribution of Dysregulated Translation Initiation to Cancer Therapy Resistance
Authors: Sunavala-Dossabhoy Gulshan
Published: December 2021

Translation of cellular RNA to protein is an energy-intensive process through which synthesized proteins dictate cellular processes and function. Translation is regulated in response to extracellular effectors and availability of amino acids intracellularly. Most eukaryotic mRNA rely on the methyl 7-guanosine (m7G) nucleotide cap to recruit the translation machinery, and the uncoupling of translational control that occurs in tumorigenesis plays a significant role in cancer treatment response. This article provides an overview of the mammalian translation initiation process and the primary mechanisms by which it is regulated. An outline of how deregulation of initiation supports tumorigenesis and how initiation at a downstream open reading frame (ORF) of Tousled-like kinase 1 (TLK1) leads to treatment resistance is discussed.
Distance Compression in the HTC Vive Pro: A Quick Revisitation of Resolution
Authors: Buck Lauren , Paris Richard , Bodenheimer Bobby
Published: December 2021

Spatial perception in immersive virtual environments, particularly regarding distance perception, is a well-studied topic in virtual reality literature. Distance compression, or the underestimation of distances, is and has been historically prevalent in all virtual reality systems. The problem of distance compression still remains open, but recent advancements have shown that as systems have developed, the level of distance compression has decreased. Here, we add evidence to this trend by beginning the assessment of distance compression in the HTC Vive Pro. To our knowledge, there are no archival results that report any findings about distance compression in this system. Using a familiar paradigm for studying distance compression in virtual reality hardware, we asked users to blind walk to a target object placed in a virtual environment and assessed their judgments based on those distances. We find that distance compression in the HTC Vive Pro mirrors that of the HTC Vive. Our results are not particularly surprising, considering the nature of the differences between the two systems, but they lend credence to the finding that resolution does not affect distance compression. More extensive study should be performed to reinforce these results.
Flood Recovery Outcomes and Disaster Assistance Barriers for Vulnerable Populations
Authors: Wilson Bradley , Tate Eric , Emrich Christopher T.
Published: December 2021

Disaster recovery spending for major flood events in the United States is at an all-time high. Yet research examining equity in disaster assistance increasingly shows that recovery funding underserves vulnerable populations. Based on a review of academic and grey literature, this article synthesizes empirical knowledge of population disparities in access to flood disaster assistance and outcomes during disaster recovery. The results identify renters, low-income households, and racial and ethnic minorities as populations that most face barriers accessing federal assistance and experience adverse recovery outcomes. The analysis explores the drivers of these inequities and concludes with a focus on the performance of disaster programs in addressing unmet needs, recognition of intersectional social vulnerabilities in recovery analysis, and gaps in data availability and transparency.
The Seasonal Snowfall Contributions of Different Snowstorm Types in Central New York State
Authors: Hartnett Justin J.
Published: December 2021

Located at the eastern extent of the Great Lakes snowbelt, Central New York averages some of the highest annual snowfall totals east of the Rocky Mountains. This is in large part due to the variety of snowstorms that affect the region including lake-effect storms, coastal storms, and overrunning storms. Previous estimates suggest that lake-effect snowstorms account for approximately half of the seasonal snow in the Great Lakes basin, but ignore the spatial variability that exists within the region. Therefore, this study examines the seasonal snowfall contributions of the different snowstorm types to affect Central New York. Results suggest that although lake-effect snowstorms are the dominant snowstorm type in the region, their seasonal snowfall contributions vary between 13 and 48%. Although lake-effect snowstorms produce more snow during the peak and mid-seasons, their relative contribution is greatest during the early and mid-winter seasons. Generally, higher contributions occur near the Tug Hill Plateau, with lower contributions in southern Central New York. Instead, snowfall in southern Central New York is mostly dominated by Nor'easters (16–35%), with lesser contributions from Rocky lows (14–29%). Overrunning storms that originate in Canada (e.g., Alberta clippers) and non-cyclonic storms contribute the least to seasonal snowfall totals across Central New York; however, they are often the catalyst for lake-effect snowstorms in the region, as they advect continental polar air masses that destabilize across the lake. Understanding the actual snowfall contribution from different snowstorm types is needed for future climate predictions. Since the potential trajectory of future snowfall varies according to the type of storm, climate models must accurately predict the type of storm that is producing the snow.
Canine Meningoencephalitis of Unknown Origin—The Search for Infectious Agents in the Cerebrospinal Fluid via Deep Sequencing
Authors: Nessler Jasmin Nicole , Jo Wendy Karen , Osterhaus Albert D. M. E. , Ludlow Martin , Tipold Andrea
Published: December 2021

Meningoencephalitis of unknown origin (MUO) describes a group of meningoencephalitides in dogs with a hitherto unknown trigger. An infectious agent has been suggested as one possible trigger of MUO but has not been proven so far. A relatively new method to screen for viral RNA or DNA is next-generation sequencing (NGS) or deep sequencing. In this study, a metagenomics analysis of the virome in a sample is analyzed and scanned for known or unknown viruses. We examined fresh-frozen CSF of 6 dogs with MUO via NGS using a modified sequence-independent, single-primer amplification protocol to detect a possible infectious trigger. Analysis of sequencing reads obtained from the six CSF samples showed no evidence of a virus infection. The inability to detect a viral trigger which could be implicated in the development of MUO in the examined population of European dogs, suggests that the current techniques are not sufficiently sensitive to identify a possible virus infection, that the virus is already eliminated at the time-point of disease outbreak, the trigger might be non-infectious or that there is no external trigger responsible for initiating MUO in dogs.
Human Anelloviruses: Prevalence and Clinical Significance During Pregnancy
Authors: Kyathanahalli Chandrashekara , Snedden Madeline , Hirsch Emmet
Published: December 2021

Although the bacterial microbiota of various compartments (e.g. vagina, amniotic fluid, and placenta) have been studied in pregnancy, there has been far less emphasis on normal and pathological viral communities. Cumulative evidence shows the presence of a number of apathogenic viruses in various tissues of healthy people, including pregnant individuals. What role, if any, these viruses play in human physiology is unknown. Anelloviruses (family Anelloviridae) are circular, single-stranded DNA viruses commonly detected with high prevalence in vertebrate hosts, including primates. Humans are nearly always colonized with at least 1 of 3 anellovirus subtypes, namely Alphatorquevirus (torque teno virus, TTV), Betatorquevirus (torque teno midi virus, TTMDV), and Gammatorquevirus (torque teno mini virus, TTMV). In healthy pregnant people, the prototype anellovirus, TTV, has been found in maternal and (variably) fetal blood, amniotic fluid, cervical and vaginal secretions, breast milk, and saliva. Nonetheless, the relevance of human anelloviruses in pregnancy and labor is unclear. There is evidence suggesting a link between anellovirus colonization and preterm birth. In this review, we discuss what is known about this family of commensal viruses in health and disease, and specifically the roles they might play during pregnancy and in the timing of delivery.
Quantitative Characterization of Complex Systems—An Information Theoretic Approach
Authors: Akundi Aditya , Smith Eric
Published: December 2021

A significant increase in System-of-Systems (SoS) is currently observed in the social and technical domains. As a result of the increasing number of constituent system components, Systems of Systems are becoming larger and more complex. Recent research efforts have highlighted the importance of identifying innovative statistical and theoretical approaches for analyzing complex systems to better understand how they work. This paper portrays the use of an agnostic two-stage examination structure for complex systems aimed towards developing an information theory-based approach to analyze complex technical and socio-technical systems. Towards the goal of characterizing system complexity with information entropy, work was carried out in exploring the potential application of entropy to a simulated case study to illustrate its applicability and to establish the use of information theory within the broad horizon of complex systems. Although previous efforts have been made to use entropy for understanding complexity, this paper provides a basic foundation for identifying a framework to characterize complexity, in order to analyze and assess complex systems in different operational domains.