bluuebunny/crossref_metadata_2025 huggingface dataset:
Transplanting Date Estimation Using Sentinel-1 Satellite Data for Paddy Rice Damage Assessment in Indonesia
Authors: Manago Naohiro , Hongo Chiharu , Sofue Yuki , Sigit Gunardi , Utoyo Budi
Published: December 2020

In Indonesia, there is a need to improve the efficiency of damage assessments of the agricultural insurance system for paddy rice producers affected by floods, droughts, pests, and diseases. In this study, we develop a method to estimate the transplanting date required for damage assessments of paddy rice fields. The study area is the Cihea irrigation district in West Java, Republic of Indonesia. Backscattering coefficients of VH polarization measured by a synthetic aperture radar onboard the Sentinel-1 satellite were used for the estimations. We investigated the accuracy of the estimations of the proposed method by smoothing out the time-series data, applying a speckle filter, and by signal synthesis of the surrounding fields. It was found that these variations effectively improved the estimation accuracy. To further improve the estimation accuracy, the data for all incident angles were used after correcting the incident angle dependence of the backscattering coefficients for three types of data with different incident angles (32°, 41°, and 45°) obtained in the study area. The estimated transplanting date for each field in the test site was compared with the transplanting date obtained through interviews. The standard deviations of the estimation errors for the four cropping periods from March 2018 to February 2020 were found to be ~5–6 days, and the percentages of estimation errors in transplanting dates within 5, 10, and 15 days were estimated to be 69%, 92%, and 97%, respectively. It was confirmed that a sufficiently reliable transplanting date estimation can be obtained ~10–15 days after transplantation.
Climate Change and the Herbicide Paradigm: Visiting the Future
Authors: Ziska Lewis H.
Published: December 2020

Weeds are recognized globally as a major constraint to crop production and food security. In recent decades, that constraint has been minimized through the extensive use of herbicides in conjunction with genetically modified resistant crops. However, as is becoming evident, such a stratagem is resulting in evolutionary selection for widespread herbicide resistance and the need for a reformation of current practices regarding weed management. Whereas such a need is recognized within the traditional auspices of weed science, it is also imperative to include emerging evidence that rising levels of carbon dioxide (CO2) and climatic shifts will impose additional selection pressures that will, in turn, affect herbicide efficacy. The goal of the current perspective is to provide historical context of herbicide use, outline the biological basis for CO2/climate impacts on weed biology, and address the need to integrate this information to provide a long-term sustainable paradigm for weed management.
Taxonomic Distribution of Neoplasia among Non-Domestic Felid Species under Managed Care
Authors: Moresco Anneke , Muñoz Karina , Gutiérrez Federico , Arias-Bernal Leonardo , Yarto-Jaramillo Enrique , Teixeira Rodrigo , Peña-Stadlin Juliana , Troan Brigid
Published: December 2020

As evidenced by numerous case reports from zoos, neoplasia in felids is common, but most reports are limited to Panthera species in North America or Europe. In order to obtain a wider epidemiologic understanding of neoplasia distribution, necropsy records at seven facilities (USA, Mexico, Colombia, Peru, and Brazil) were evaluated. In contrast to others, this study population (195 cases, 16 species), included many non-Panthera felids. Overall neoplasia prevalence was 28.2% (55/195). Panthera species had a higher prevalence of neoplasia than non-Panthera species (52.5%; vs. 13.0%). Lions (66.7%), jaguars (55.0%), and tigers (31.3%) had the highest species-specific prevalence of neoplasia. Neoplasms in Panthera species were more frequently malignant than in non-Panthera (86.1% vs. 55.6%). The systems most commonly affected were the reproductive, hematolymphoid, and respiratory. The range of management conditions and more varied genetic backgrounds support a robust taxonomic pattern and suggest that the reported propensity for neoplasia in jaguars may have a genetic basis at a taxonomic level higher than species, as lions and tigers also have high prevalence. Given the high prevalence of neoplasia and high likelihood of malignancy, routine medical exams in all nondomestic felids, but Panthera species in particular, should include thorough assessments of any clinical signs of neoplasia.
Feed Intake, Methane Emissions, Milk Production and Rumen Methanogen Populations of Grazing Dairy Cows Supplemented with Various C 18 Fatty Acid Sources
Authors: Boland Tommy M. , Pierce Karina M. , Kelly Alan K. , Kenny David A. , Lynch Mary B. , Waters Sinéad M. , Whelan Stephen J. , McKay Zoe C.
Published: December 2020

Emissions of methane (CH4) from dairy production systems are environmentally detrimental and represent an energy cost to the cow. This study evaluated the effect of varying C18 fatty acid sources on CH4 emissions, milk production and rumen methanogen populations in grazing lactating dairy cows. Forty-five Holstein Friesian cows were randomly allocated to one of three treatments (n = 15). Cows were offered 15 kg dry matter (DM)/d of grazed pasture plus supplementary concentrates (4 kg DM/d) containing either stearic acid (SA), linseed oil (LO), or soy oil (SO). Cows offered LO and SO had lower pasture DM intake (DMI) than those offered SA (11.3, 11.5 vs. 12.6 kg/d). Cows offered LO and SO had higher milk yield (21.0, 21.3 vs. 19.7 kg/d) and milk protein yield (0.74, 0.73 vs. 0.67 kg/d) than those offered SA. Emissions of CH4 (245 vs. 293, 289 g/d, 12.4 vs. 15.7, 14.8 g/kg of milk and 165 vs. 207, 195 g/kg of milk solids) were lower for cows offered LO than those offered SA or SO. Methanobrevibacter ruminantium abundance was reduced in cows offered LO compared to SA. Offering supplementary concentrates containing LO can reduce enteric CH4 emissions from pasture fed dairy cows.
Effects of Cold Exposure on Some Physiological, Productive, and Metabolic Variables in Lactating Dairy Goats
Authors: Coloma-García Wellington , Mehaba Nabil , Such Xavier , Caja Gerardo , Salama Ahmed A. K.
Published: December 2020

Low winter temperatures in some regions have a negative impact on animal performance, behavior, and welfare. The objective of this study was to evaluate some physiological, metabolic, and lactational responses of dairy goats exposed to cold temperatures for 3 weeks. Eight Murciano-Granadina dairy goats (41.8 kg body weight, 70 days in milk, and 2.13 kg/day milk) were used from mid-January to mid-March. Goats were divided into 2 balanced groups and used in a crossover design with 2 treatments in 2 periods (21 days each, 14 days adaptation and 7 days for measurements). After the first period, goats were switched to the opposite treatment. The treatments included 2 different controlled climatic conditions with different temperature-humidity index (THI) values. The treatments were: thermoneutral conditions (TN; 15 to 20 °C, 45% humidity, THI = 58 to 65), and cold temperature (CT; −3 to 6 °C, 63% humidity, THI = 33 to 46). Goats were fed ad libitum a total mixed ration (70% forage and 30% concentrate) and water was freely available. Goats were milked at 0800 and 1700 h. Dry matter intake, water consumption, rectal temperature, and respiratory rate were recorded daily (days 15 to 21). Body weight was recorded at the start and end of each period. Milk samples for composition were collected on 2 consecutive days (days 20 and 21). Insulin, glucose, non-esterified fatty acids (NEFA), ß-hydroxybutyrate (BHB), cholesterol, and triglycerides were measured in blood on d 21. Compared to TN goats, CT goats had similar feed intake, but lower water consumption (−22 ± 3%), respiratory rate (−5 ± 0.8 breaths/min), and rectal temperature (−0.71 ± 0.26 °C). Milk yield decreased by 13 ± 3% in CT goats, but their milk contained more fat (+13 ± 4%) and protein (+14 ± 5%), and consequently the energy-corrected milk did not vary between TN and CT goats. The CT goats lost 0.64 kg of body weight, whereas TN goats gained 2.54 kg in 21 days. Blood insulin and cholesterol levels were not affected by CT. However, values of blood glucose, NEFA, hematocrit, and hemoglobin increased or tended to increase by CT, whereas BHB and triglycerides decreased. Overall, CT goats produced less but concentrated milk compared to TN goats. Despite similar feed intake and blood insulin levels CT goats had increased blood glucose and NEFA levels. The tendency of increased blood NEFA indicates that CT goats mobilized body fat reserves to cover the extra energy needed for heat production under cold conditions.
Are Follow-Up Blood Cultures Useful in the Antimicrobial Management of Gram Negative Bacteremia? A Reappraisal of Their Role Based on Current Knowledge
Authors: Cogliati Dezza Francesco , Curtolo Ambrogio , Volpicelli Lorenzo , Ceccarelli Giancarlo , Oliva Alessandra , Venditti Mario
Published: December 2020

Bloodstream infections still constitute an outstanding cause of in-hospital morbidity and mortality, especially among critically ill patients. Follow up blood cultures (FUBCs) are widely recommended for proper management of Staphylococcus aureus and Candida spp. infections. On the other hand, their role is still a matter of controversy as far as Gram negative bacteremias are concerned. We revised, analyzed, and commented on the literature addressing this issue, to define the clinical settings in which the application of FUBCs could better reveal its value. The results of this review show that critically ill patients, endovascular and/or non-eradicable source of infection, isolation of a multi-drug resistant pathogen, end-stage renal disease, and immunodeficiencies are some factors that may predispose patients to persistent Gram negative bacteremia. An analysis of the different burdens that each of these factors have in this clinical setting allowed us to suggest which patients’ FUBCs have the potential to modify treatment choices, prompt an early source control, and finally, improve clinical outcome.
Feasibility and Validity of a Framework for Antimicrobial Stewardship in General Practice: Key Stakeholder Interviews
Authors: Hawes Lesley A. , Bishop Jaclyn , Buising Kirsty , Mazza Danielle
Published: December 2020

There is little guidance about developing systems for antimicrobial stewardship (AMS) for general practice. A literature review identified six key components: governance, monitoring of antibiotic prescribing and resistance with feedback to prescribers, consultation support, education of the public and general practitioners, pharmacist and nurse involvement, and research, which were incorporated into a potential framework for the general practice context. Objectives: to determine the feasibility and validity of the proposed AMS framework. A secondary objective was to identify likely bodies responsible for implementation in Australia. We undertook interviews with 12 key stakeholders from government, research, and professional groups. Data were analysed with a thematic approach. The framework was considered valid and feasible. No clear organisation was identified to lead AMS implementation in general practice. The current volume-based antibiotic prescription monitoring system was considered insufficient. AMS education for the public, further development of GP education, and improved consultation support were strongly recommended. The role of community-based pharmacists and nurses is largely unexplored, but their involvement was recommended. A clear leader to drive AMS in general practice is essential for an action framework to gain traction. Monitoring and feedback of antibiotic prescribing require urgent development to include monitoring of prescribing appropriateness and patient outcomes.
Powerful Antibacterial Peptides from Egg Albumin Hydrolysates
Authors: Al-Mohammadi Abdul-Raouf , Osman Ali , Enan Gamal , Abdel-Shafi Seham , El-Nemer Mona , Sitohy Mahmoud , Taha Mohamed A.
Published: December 2020

Native egg albumin (NEA) was isolated from hen eggs and hydrolyzed by pepsin to produce hydrolyzed egg albumin (HEA). HEA was chemically characterized and screened for its antibacterial activity against 10 pathogenic bacteria (6 Gram (+) and 4 Gram (−)). The SDS-PAGE pattern of NEA showed molecular weights of hen egg albumin subunits ranging from 30 to 180 kDa. The highest intensive bands appeared at a molecular mass of about 50 and 97 kDa. Ultra-performance liquid chromatography (UPLC) of the peptic HEA revealed 44 peptides, 17 of them were dipeptides, and the other 27 fractions corresponded to bigger peptides (3–9 amino acids). The dipeptides and big peptides represented 26% and 74% of the total hydrolysate, respectively. The MIC of HEA was about 100 μg/L for Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Salmonella typhimurium, Streptococcus pyogenes, and Klebsiella oxytoca and 150 μg/L for Pseudomonas aeruginosa, Bacillus subtilis, and Listeria ivanovii and 200 μg/L for Escherichia coli. L. monocytogenes was the most sensitive organism to HEA. Mixtures of HEA with antibiotics showed more significant antibacterial activity than individually using them. Transmission electron microscopy (TEM) revealed various signs of cellular deformation in the protein-treated bacteria. HEA may electrostatically and hydrophobically interact with the cell wall and cell membrane of the susceptible bacteria, engendering large pores and pore channels leading to cell wall and cell membrane disintegration. Higher cell permeability may, thus, occur, leading to cell emptiness, lysis, and finally death. Alternatively, no toxicity signs appeared when HEA was administrated to Wistar Albino rats as one single dose (2000, 5000 mg/kg body weight) or repeated daily dose (500 and 2500 mg/kg body weight/day) for 28 days to disclose the possible toxicity hazards. HEA did not produce any death.
Nigella sativa and Thymoquinone: A Natural Blessing for Periodontal Therapy
Authors: Mekhemar Mohamed , Hassan Yasmine , Dörfer Christof
Published: December 2020

Thymoquinone (TQ), the chief active constituent of Nigella sativa (NS), shows very valuable biomedical properties such as antioxidant, antimicrobial, anticancer, anti-inflammatory, antihypertensive, hypoglycemic, antiparasitic and anti-asthmatic effects. Several studies have examined the pharmacological actions of TQ in the treatment of oral diseases but its potential role in periodontal therapy and regeneration is not yet fully defined. The present investigation has been designed to review the scientific studies about the effects of TQ as an adjunct to periodontal treatment to promote healing and periodontal regeneration. Along with clinical experiments, in vitro studies exhibit the beneficial effects of TQ during periodontal therapy. Nevertheless, additional comprehensive clinical and preclinical studies at cellular and molecular levels are essential to examine the particular action mechanisms of Nigella sativa and its elements, particularly TQ, during periodontal treatment or regeneration.
Phytosterols: Nutritional Health Players in the Management of Obesity and Its Related Disorders
Authors: Vezza Teresa , Canet Francisco , de Marañón Aranzazu M. , Bañuls Celia , Rocha Milagros , Víctor Víctor Manuel
Published: December 2020

Obesity and its related disorders, such as diabetes and cardiovascular risk, represent an emerging global health issue. Even though genetic factors seem to be the primary actors in the development and progression of these diseases, dietary choices also appear to be of crucial importance. A healthy diet combined with physical activity have been shown to ameliorate glycaemic levels and insulin sensitivity, reduce body weight and the risk of chronic diseases, and contribute to an overall improvement in quality of life. Among nutrients, phytosterols have become the focus of growing attention as novel functional foods in the management of metabolic disorders. Phytosterols are natural plant compounds belonging to the triterpene family and are structurally similar to cholesterol. They are known for their cholesterol-lowering effects, anti-inflammatory and antioxidant properties, and the benefits they offer to the immune system. The present review aims to provide an overview of these bioactive compounds and their therapeutic potential in the fields of obesity and metabolic disorders, with special attention given to oxidative stress, inflammatory status, and gut dysbiosis, all common features of the aforementioned diseases.
Dynamic Modeling and Simulation of a Robotic Lander Based on Variable Radius Drums
Authors: Caruso Matteo , Scalera Lorenzo , Gallina Paolo , Seriani Stefano
Published: December 2020

Soft-landing on planetary surfaces is the main challenge in most space exploration missions. In this work, the dynamic modeling and simulation of a three-legged robotic lander based on variable radius drums are presented. In particular, the proposed robotic system consists of a non-reversible mechanism that allows a landing object to constant decelerate in the phase of impact with ground. The mechanism is based on variable radius drums, which are used to shape the elastic response of a spring to produce a specific behavior. A dynamic model of the proposed robotic lander is first presented. Then, its behavior is evaluated through numerical multibody simulations. Results show the feasibility of the proposed design and applicability of the mechanism in landing operations.
Effect of Wood Fiber Loading on the Chemical and Thermo-Rheological Properties of Unrecycled and Recycled Wood-Polymer Composites
Authors: Pušnik Črešnar Klementina , Fras Zemljič Lidija , Slemenik Perše Lidija , Bek Marko
Published: December 2020

Novel wood fiber (WF)-polypropylene composites were developed using the extrusion process with a twin-screw extruder. The influence of different mass addition of WF to unrecycled polypropylene (PP) and recycled PP (R-PP) on the chemical, thermal and rheological properties of the processed WF-PP and WF-R-PP composites was investigated. For this purpose, the chemical surface structure of the composites was followed with ATR-FTIR (attenuated total reflection Fourier transform infra red spectroscopy), while the thermal properties of the WF-PP composites were investigated with differential scanning calorimetry (DSC). Furthermore, the crystalline structure of the composites was determined by X-ray diffraction (XRD) analysis. Finally, the rheology of the materials was also studied. It was observed that a stronger particle formation at high additional concentrations was observed in the case of recycled PP material. The addition of WF over 20% by weight increased the crystallinity as a result of the incorporation and reorganization of the WF and also their reinforcing effect. The addition of WF to pure PP had an influence on the crystallization process, which due to the new β phase and γ phase PP formation showed an increased degree of crystallinity of the composites and led to a polymorphic structure of the composites WF-PP. From the rheological test, we can conclude that the addition of WF changed the rheological behavior of the material, as WF hindered the movement of the polymeric material. At lower concentrations, the change was less pronounced, although we observed more drastic changes in the material behavior at concentrations high enough that WF could form a 3D network (percolation point about 20%).
Enhancement of Power System Transient Stability by Active and Reactive Power Control of Variable Speed Wind Generators
Authors: Yagami Masaki , Ichinohe Masanori , Tamura Junji
Published: December 2020

This paper proposes a novel control method for enhancing transient stability by using renewable energy sources (RES). The kinetic energy accumulated in a rotor of variable speed wind generator (VSWG) is proactively used as the active power source, which is controlled according to the frequency measured at the wind farm. In addition, coordinated reactive power control according to the grid voltage is also carried out to more effectively use the kinetic energy of the VSWG. The effects of the proposed control system were evaluated by simulation analyses performed using a modified IEEE nine-bus power system network made up of synchronous generators (SGs), a photovoltaic (PV) system and a VSWG-based wind farm. Furthermore, the coordinated reactive power control between the VSWG and PV system was also demonstrated.
Robust Adaptive Synchronization of a Class of Uncertain Chaotic Systems with Unknown Time-Delay
Authors: Zare Assef , Mirrezapour Seyede Zeynab , Hallaji Majid , Shoeibi Afshin , Jafari Mahboobeh , Ghassemi Navid , Alizadehsani Roohallah , Mosavi Amir
Published: December 2020

In this paper, a robust adaptive control strategy is proposed to synchronize a class of uncertain chaotic systems with unknown time delays. Using Lyapunov theory and Lipschitz conditions in chaotic systems, the necessary adaptation rules for estimating uncertain parameters and unknown time delays are determined. Based on the proposed adaptation rules, an adaptive controller is recommended for the robust synchronization of the aforementioned uncertain systems that prove the robust stability of the proposed control mechanism utilizing the Lyapunov theorem. Finally, to evaluate the proposed robust and adaptive control mechanism, the synchronization of two Jerk chaotic systems with finite non-linear uncertainty and external disturbances as well as unknown fixed and variable time delays are simulated. The simulation results confirm the ability of the proposed control mechanism in robust synchronization of the uncertain chaotic systems as well as to estimate uncertain and unknown parameters.
Assessing Groundwater Level with a Unified Seasonal Outlook and Hydrological Modeling Projection
Authors: Huang Jing-Ying , Shih Dong-Sin
Published: December 2020

Although the annual rainfall in Taiwan is high, water shortages still occasionally occur owing to its nonuniform temporal and spatial distribution. At these times, the groundwater is considered an acceptable alternative water source. Groundwater is of particular value because it is considered a clean and reliable source of fresh water. To prevent water scarcity, this study utilized seasonal forecasting by incorporating hydrological models to evaluate the seasonal groundwater level. The seasonal prospective issued by the Central Weather Bureau of Taiwan (CWB) was combined with weather generator data to construct seasonal weather forecasts as the input for hydrological models. A rainfall-runoff model, HEC-HMS, and a coupled groundwater and surface water model, WASH123D, were applied to simulate the seasonal groundwater levels. The Fengshan Creek basin in northern Taiwan was selected as a study site to test the proposed approach. The simulations demonstrated stability and feasibility, and the results agreed with the observed groundwater table. The calibrations indicated that the average errors of river stage were 0.850 for R2, 0.279 for root-mean-square error (RMSE), and 0.824 for efficiency coefficient (CE). The simulation also revealed that the simulated groundwater table corresponded with observed hydrographs very well (R2 of 0.607, RMSE of 0.282 m, and CE of 0.621). The parameters were verified in this study, and they were deemed practical and adequate for subsequent seasonal assessment. The seasonal forecast of 2018 at Guanxi station indicated that the 25th and 75th percentiles of simulated annual rainfall were within 1921–3285 mm and the actual annual rainfall was 2031 mm. Its seasonal rainfall outlook was around 30% accurate for forecasts of three consecutive months in 2018. Similarly, at Xinpu station, its seasonal rainfall outlook was about 40% accurate, and the amount of annual rainfall (1295 mm) was within the range of the 25th and 75th percentiles (1193–1852 mm). This revealed that the actual annual precipitations at both Guanxi and Xinpu station corresponded with the range of 25th and 75th percentiles of simulated rainfall, even if the accurate rate for the 3 month seasonal forecast had some error. The subsequent groundwater simulations were overestimated because the amount of actual rainfall was far lower than the average of the historical record in some dry season months. However, the amount of rainfall returned to normal values during the wet seasons, where the seasonal forecast and observation results were similar.
Generic Digital Twin Architecture for Industrial Energy Systems
Authors: Steindl Gernot , Stagl Martin , Kasper Lukas , Kastner Wolfgang , Hofmann Rene
Published: December 2020

Digital Twins have been in the focus of research in recent years, trying to achieve the vision of Industry 4.0. In the domain of industrial energy systems, they are applied to facilitate a flexible and optimized operation. With the help of Digital Twins, the industry can participate even stronger in the ongoing renewable energy transition. Current Digital Twin implementations are often application-specific solutions without general architectural concepts and their structures and namings differ, although the basic concepts are quite similar. For this reason, we analyzed concepts, architectures, and frameworks for Digital Twins in the literature to develop a technology-independent Generic Digital Twin Architecture (GDTA), which is aligned with the information technology layers of the Reference Architecture Model Industry 4.0 (RAMI4.0). This alignment facilitates a common naming and understanding of the proposed architectural structure. A proof-of-concept shows the application of Semantic Web technologies for instantiating the proposed GDTA for a use case of a Packed-Bed Thermal Energy Storage (PBTES).
Circulation Patterns Associated with Current and Future Rainfall over Ethiopia and South Sudan from a Convection-Permitting Model
Authors: Misiani Herbert O. , Finney Declan L. , Segele Zewdu T. , Marsham John H. , Tadege Abebe , Artan Guleid , Atheru Zachary
Published: December 2020

Ethiopia and South Sudan contain several population centers and important ecosystems that depend on July–August rainfall. Here we use two models to understand current and future rainfall: the first ever pan-African numerical model of climate change with explicit convection and a parameterized model that resembles a typical regional climate model at 4.5 and 25 km horizontal grid-spacing, respectively. The explicit convection and higher resolution of the first model offer a greatly improved representation of both the frequency and intensity of rainfall, when compared to the parametrized convection model. Furthermore, only this model has success in capturing the east–west propagation of rainfall over the full diurnal cycle. Enhanced low-level westerlies were found for extremely wet days, though this response was weaker in the explicit convection model. The increased orographic detail in the explicit model resulted in the splitting of the low-level Turkana Jet core into smaller cores, and inhibited its penetration far into South Sudan. Some projected changes were found to be independent of model, such as changes in the strength of Somali and Turkana jets, as well as the shifting of Turkana jet core to lower levels. However, the explicit model end-of-century projections showed a larger and clearer decrease in wet days, accompanied by an increase in wet day intensity and extreme rainfall. This study highlights serious limitations of relying solely on simulations which parameterize convection to inform decisions in the region of South Sudan and Ethiopia.
Effect of Various Types of ENSO Events on Moisture Conditions in the Humid and Subhumid Tropics
Authors: Gushchina Daria , Zheleznova Irina , Osipov Alexander , Olchev Alexander
Published: December 2020

Moisture anomaly conditions within humid and subhumid tropics that are associated with different types of El Niño and La Niña phenomena are described and analyzed with a focus on their spatial distribution and seasonal variability. Five dryness indices (Keetch–Byram Drought Index, Weighted Anomaly Standardized Precipitation Index, Standardized Precipitation Index, Palmer Drought Severity Index, and Percent of Normal Precipitation) were derived from ECMWF (European Centre for Medium-Range Weather Forecasts) fifth generation reanalysis (ERA5) reanalysis and University Corporation for Atmospheric Research (UCAR) datasets for the period from 1979 to 2019. Cross-correlation analysis was used to evaluate the relationships between the El Niño Southern Oscillation (ENSO) and selected dryness indices. To describe the seasonal variability of the ENSO–surface moisture relationships, the composite maps of dryness indices in different seasons were analyzed. The results showed a significant heterogeneity of the ENSO-induced moisture anomaly conditions both within and across various geographical regions. Four main areas in humid and subhumid tropics with the maximum effects of El Niño/La Niña events on the surface moisture conditions were found: Southeast Asia and Australia, Eastern and South Africa, Northeastern and Eastern South America, and Central America. It was shown that the effects of La Niña were usually opposite to those of El Niño, while the responses to the two types of El Niño differed mostly in the moisture anomaly intensity and its spatial patterns.
Annotation-Assisted Clustering of Player Profiles in Cultural Games: A Case for Tensor Analytics in Julia
Authors: Drakopoulos Georgios , Voutos Yorghos , Mylonas Phivos
Published: December 2020

Computer games play an increasingly important role in cultural heritage preservation. They keep tradition alive in the digital domain, reflect public perception about historical events, and make history, and even legends, vivid, through means such as advanced storytelling and alternative timelines. In this context, understanding the respective underlying player base is a major success factor as different game elements elicit various emotional responses across players. To this end, player profiles are often built from a combination of low- and high-level attributes. The former pertain to ordinary activity, such as collecting points or badges, whereas the latter to the outcome of strategic decisions, such as participation in in-game events such as tournaments and auctions. When available, annotations about in-game items or player activity supplement these profiles. In this article, we describe how such annotations may be integrated into different player profile clustering schemes derived from a template Simon–Ando iterative process. As a concrete example, the proposed methodology was applied to a custom benchmark dataset comprising the player base of a cultural game. The findings are interpreted in the light of Bartle taxonomy, one of the most prominent player categorization. Moreover, the clustering quality is based on intra-cluster distance and cluster compactness. Based on these results, recommendations in an affective context for maximizing engagement are proposed for the particular game player base composition.
Tissue Acidosis Associated with Ischemic Stroke to Guide Neuroprotective Drug Delivery
Authors: M. Tóth Orsolya , Menyhárt Ákos , Frank Rita , Hantosi Dóra , Farkas Eszter , Bari Ferenc
Published: December 2020

Ischemic stroke is a leading cause of death and disability worldwide. Yet, the effective therapy of focal cerebral ischemia has been an unresolved challenge. We propose here that ischemic tissue acidosis, a sensitive metabolic indicator of injury progression in cerebral ischemia, can be harnessed for the targeted delivery of neuroprotective agents. Ischemic tissue acidosis, which represents the accumulation of lactic acid in malperfused brain tissue is significantly exacerbated by the recurrence of spreading depolarizations. Deepening acidosis itself activates specific ion channels to cause neurotoxic cellular Ca2+ accumulation and cytotoxic edema. These processes are thought to contribute to the loss of the ischemic penumbra. The unique metabolic status of the ischemic penumbra has been exploited to identify the penumbra zone with imaging tools. Importantly, acidosis in the ischemic penumbra may also be used to guide therapeutic intervention. Agents with neuroprotective promise are suggested here to be delivered selectively to the ischemic penumbra with pH-responsive smart nanosystems. The administered nanoparticels release their cargo in acidic tissue environment, which reliably delineates sites at risk of injury. Therefore, tissue pH-targeted drug delivery is expected to enrich sites of ongoing injury with the therapeutical agent, without the risk of unfavorable off-target effects.
Difference between Selenite and Selenate in the Regulation of Growth and Physiological Parameters of Nickel-Exposed Lettuce
Authors: Hawrylak-Nowak Barbara , Matraszek-Gawron Renata
Published: December 2020

Nickel is an essential plant micronutrient; however, even at low concentrations, it may be phytotoxic. Selenium is a beneficial element with an alleviating effect that has been confirmed in the case of many abiotic stresses, including metal toxicity. The aim of this study is to assess the effect of two forms of Se (Se(IV) or Se(VI)) on the phytotoxicity, accumulation, and translocation of Ni in lettuce. Nickel causes a reduction in lettuce growth and vitality of roots, probably through increased lipid peroxidation. The application of Se(IV) to a Ni-contaminated medium resulted in a further reduction of growth, especially in the presence of 6 µM Se(IV). The growth-promoting effect of Se was found only in the 2 µM Se(VI)/10 µM Ni treatment. The application of 6 µM Se, regardless of the Se form, to the Ni-containing substrate caused an increase in shoot Ni concentration. In turn, a decrease in root Ni content was found for all Se treatments. The strong aggravation of Ni phytotoxicity in the presence of 6 µM Se(IV) was most likely related to the accumulation of high Se concentration in the roots, and the combination of high root Ni accumulation caused irreversible dysregulation of cell metabolism.
Cytokinin-Regulated Expression of Arabidopsis thaliana PAP Genes and Its Implication for the Expression of Chloroplast-Encoded Genes
Authors: Andreeva Aleksandra A. , Vankova Radomira , Bychkov Ivan A. , Kudryakova Natalia V. , Danilova Maria N. , Lacek Jozef , Pojidaeva Elena S. , Kusnetsov Victor V.
Published: December 2020

Cytokinins (CKs) are known to regulate the biogenesis of chloroplasts under changing environmental conditions and at different stages of plant ontogenesis. However, the underlying mechanisms are still poorly understood. Apparently, the mechanisms can be duplicated in several ways, including the influence of nuclear genes that determine the expression of plastome through the two-component CK regulatory circuit. In this study, we evaluated the role of cytokinins and CK signaling pathway on the expression of nuclear genes for plastid RNA polymerase-associated proteins (PAPs). Cytokinin induced the expression of all twelve Arabidopsis thalianaPAP genes irrespective of their functions via canonical CK signaling pathway but this regulation might be indirect taking into consideration their different functions and versatile structure of promoter regions. The disruption of PAP genes contributed to the abolishment of positive CK effect on the accumulation of the chloroplast gene transcripts and transcripts of the nuclear genes for plastid transcription machinery as can be judged from the analysis of pap1 and pap6 mutants. However, the CK regulatory circuit in the mutants remained practically unperturbed. Knock-out of PAP genes resulted in cytokinin overproduction as a consequence of the strong up-regulation of the genes for CK synthesis.
Efficient Portable Urea Biosensor Based on Urease Immobilized Membrane for Monitoring of Physiological Fluids
Authors: Kim Jee Young , Sung Gun Yong , Park Min
Published: December 2020

Numerous studies have addressed the utilization of glutaraldehyde (GA) as a homobifunctional cross-linker. However, its applicability has been impeded due to several issues, including the tendency of GA molecules to undergo polymerization. Herein, a portable urea biosensor was developed for the real-time monitoring of the flow of physiological fluids; this was achieved by using disuccinimidyl cross-linker-based urease immobilization. Urease was immobilized on a porous polytetrafluoroethylene (PTFE) solid support using different disuccinimidyl cross-linkers, namely disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS) and bis-N-succinimidyl-(pentaethylene glycol) ester (BS(PEG)5). A urease activity test revealed that DSS exhibited the highest urease immobilizing efficiency, whereas FT-IR analysis confirmed that urease was immobilized on the PTFE membrane via DSS cross-linking. The membrane was inserted in a polydimethylsiloxane (PDMS) fluidic chamber that generated an electrochemical signal in the presence of a flowing fluid containing urea. Urea samples were allowed to flow into the urea biosensor (1.0 mL/min) and the signal was measured using chronoamperometry. The sensitivity of the DSS urea biosensor was the highest of all the trialed biosensors and was found to be superior to the more commonly used GA cross-linker. To simulate real-time monitoring in a human patient, flowing urea-spiked human serum was measured and the effective urease immobilization of the DSS urea biosensor was confirmed. The repeatability and interference of the urea biosensor were suitable for monitoring urea concentrations typically found in human patients.
Differential Role of Circulating microRNAs to Track Progression and Pre-Symptomatic Stage of Chronic Heart Failure: A Pilot Study
Authors: D’Alessandra Yuri , Chiesa Mattia , Carena Maria Cristina , Beltrami Antonio Paolo , Rizzo Paola , Buzzetti Marta , Ricci Veronica , Ferrari Roberto , Fucili Alessandro , Livi Ugolino , Aleksova Aneta , Pompilio Giulio , Colombo Gualtiero I.
Published: December 2020

(1)Background: Chronic heart failure (CHF) contributes to the overall burden of cardiovascular disease. Early identification of at-risk individuals may facilitate the targeting of precision therapies. Plasma microRNAs are promising circulating biomarkers for their implications with cardiac pathologies. In this pilot study, we investigate the possible exploitability of circulating micro-RNAs (miRNAs) to track chronic heart failure (CHF) occurrence, and progression from NYHA class I to IV. (2)Methods: We screened 367 microRNAs using TaqMan microRNA Arrays in plasma samples from healthy controls (HC) and CHF NYHA-class I-to-IV patients (5/group). Validation was performed by singleplex assays on 10 HC and 61 CHF subjects. Differences in the expression of validated microRNAs were evaluated through analysis of covariance (ANCOVA). Associations between N-terminal pro-BNP (NT-proBNP), left ventricular end-diastolic volume (LVEDV) or peak oxygen uptake (VO2 peak) and plasma microRNA were assessed by multivariable linear regression analysis. (3)Results: Twelve microRNAs showed higher expression in CHF patients vs. HC. Seven microRNAs were associated with NT-proBNP concentration; of these, miR-423-5p was also an independent predictor of LVEDV. Moreover, miR-499-5p was a predictor of the VO2 peak. Finally, a cluster of 5 miRNAs discriminated New York Heart Association (NYHA) class-I from HC subjects. (4)Conclusions: Our data suggest that circulating miRNAs have the potential to serve as pathophysiology-based markers of HF status and progression, and as indicators of pre-symptomatic individuals.
NF-κB Signaling Is Regulated by Fucosylation in Metastatic Breast Cancer Cells
Authors: Doud Emma H. , Shetty Trupti , Abt Melissa , Mosley Amber L. , Corson Timothy W. , Mehta Anand , Yeh Elizabeth S.
Published: December 2020

A growing body of evidence indicates that the levels of fucosylation correlate with breast cancer progression and contribute to metastatic disease. However, very little is known about the signaling and functional outcomes that are driven by fucosylation. We performed a global proteomic analysis of 4T1 metastatic mammary tumor cells in the presence and absence of a fucosylation inhibitor, 2-fluorofucose (2FF). Of significant interest, pathway analysis based on our results revealed a reduction in the NF-κB and TNF signaling pathways, which regulate the inflammatory response. NF-κB is a transcription factor that is pro-tumorigenic and a prime target in human cancer. We validated our results, confirming that treatment of 4T1 cells with 2FF led to a decrease in NF-κB activity through increased IκBα. Based on these observations, we conclude that fucosylation is an important post-translational modification that governs breast cancer cell signaling.
The Inhibition of Prolyl Oligopeptidase as New Target to Counteract Chronic Venous Insufficiency: Findings in a Mouse Model
Authors: Casili Giovanna , Lanza Marika , Scuderi Sarah Adriana , Messina Salvatore , Paterniti Irene , Campolo Michela , Esposito Emanuela
Published: December 2020

(1) Background: Chronic venous insufficiency (CVI) is a common disorder related to functional and morphological abnormalities of the venous system. Inflammatory processes and angiogenesis alterations greatly concur to the onset of varicose vein. KYP-2047 is a selective inhibitor of prolyl oligopeptidase (POP), a serine protease involved in the release of pro-angiogenic molecules. The aim of the present study is to evaluate the capacity of KYP-2047 to influence the angiogenic and inflammatory mechanisms involved in the pathophysiology of CVI. (2) Methods: An in vivo model of CVI-induced by saphene vein ligation (SVL) and a tissue block culture study were performed. Mice were subjected to SVL followed by KYP-2047 treatment (intraperitoneal, 10 mg/kg) for 7 days. Histological analysis, Masson’s trichrome, Van Gieson staining, and mast cells evaluation were performed. Release of cytokines, nitric oxide synthase production, TGF-beta, VEGF, α-smooth muscle actin, PREP, Endoglin, and IL-8 quantification were investigated. (3) Results: KYP-2047 treatment ameliorated the histological abnormalities of the venous wall, reduced the collagen increase and modulated elastin content, lowered cytokines levels and prevented mast degranulation. Moreover, a decreased expression of TGF-beta, eNOS, VEGF, α-smooth muscle actin, IL-8, and PREP was observed in in vivo study; also a reduction in VEGF and Endoglin expression was confirmed in tissue block culture study. (4) Conclusions: For the first time, this research, highlighting the importance of POP as new target for vascular disorders, revealed the therapeutic potential of KYP-2047 as a helpful treatment for the management of CVI.
A Novel Nonsense Mutation in FGB (c.1421G>A; p.Trp474Ter) in the Beta Chain of Fibrinogen Causing Hypofibrinogenemia with Bleeding Phenotype
Authors: Simurda Tomas , Vilar Rui , Zolkova Jana , Ceznerova Eliska , Kolkova Zuzana , Loderer Dusan , Neerman-Arbez Marguerite , Casini Alessandro , Brunclikova Monika , Skornova Ingrid , Dobrotova Miroslava , Grendar Marian , Stasko Jan , Kubisz Peter
Published: December 2020

Congenital hypofibrinogenemia is a rare bleeding disorder characterized by a proportional decrease of functional and antigenic fibrinogen levels. Hypofibrinogenemia can be considered the phenotypic expression of heterozygous loss of function mutations occurring within one of the three fibrinogen genes (FGA, FGB, and FGG). Clinical manifestations are highly variable; most patients are usually asymptomatic, but may appear with mild to severe bleeding or thrombotic complications. We have sequenced all exons of the FGA, FGB, and FGG genes using the DNA isolated from the peripheral blood in two unrelated probands with mild hypofibrinogenemia. Coagulation screening, global hemostasis, and functional analysis tests were performed. Molecular modeling was used to predict the defect of synthesis and structural changes of the identified mutation. DNA sequencing revealed a novel heterozygous variant c.1421G>A in exon 8 of the FGB gene encoding a Bβ chain (p.Trp474Ter) in both patients. Clinical data from patients showed bleeding episodes. Protein modelling confirmed changes in the secondary structure of the molecule, with the loss of three β sheet arrangements. As expected by the low fibrinogen levels, turbidity analyses showed a reduced fibrin polymerisation and imaging difference in thickness fibrin fibers. We have to emphasize that our patients have a quantitative fibrinogen disorder; therefore, the reduced function is due to the reduced concentration of fibrinogen, since the Bβ chains carrying the mutation predicted to be retained inside the cell. The study of fibrinogen molecules using protein modelling may help us to understand causality and effect of novel genetic mutations.
Coping Styles among People with Parkinson’s Disease: A Three-Year Follow-Up Study
Authors: Nilsson Maria H. , Oswald Frank , Palmqvist Sebastian , Slaug Björn
Published: December 2020

People with Parkinson’s disease (PD) experience a gradual loss of functional abilities that affects all facets of their daily life. There is a lack of longitudinal studies on coping styles in relation to the disease progression among people with PD. The aim of this study was to explore how coping styles in PD evolve over a 3-year period. Data from the longitudinal project “Home and Health in People Ageing with PD” was utilized (N = 158), including baseline and 3-year follow-up assessments. Coping was captured by ratings of 13 different coping styles. A factor analysis was conducted to analyse patterns of coping styles. Stability and change were analysed for each of the 13 styles with respect to the course of the disease. The factor analysis revealed four coping patterns: pessimistic, optimistic, persistent and support-seeking. The stability of each coping style over time ranged from 75.3% to 90.5%. Those who experienced a worsening of the disease were most inclined to change their coping style (p = 0.006). The results suggest that even when facing severe challenges due to PD in daily life, coping styles remain relatively stable over time. However, a worsening in PD severity appeared to trigger a certain re-evaluation of coping styles.
Experimental Data and Simulations of Performance and Thermal Comfort in a Patient Room Equipped with Radiant Ceiling Panels
Authors: Valdiserri Paolo , Cesari Silvia , Coccagna Maddalena , Romio Pasquale , Mazzacane Sante
Published: December 2020

Hospitals require the highest energy demands in non-residential buildings. They provide healthcare 24/7/365 and, at the same time, they ensure indoor air quality, thermal comfort and sterility. However, several studies reveal that high indoor temperatures and low relative humidity (RH) are often perceived in patient rooms during the heating season, suggesting an important energy saving potential. Against this background, radiant ceiling panel (RCP) systems result to be one of the most appropriate solutions as they allow to achieve significant energy savings while providing the highest level of thermal and acoustic comfort, as well as of infection control. In the present study the microclimatic survey of a patient room at Maggiore Hospital in Bologna, Italy, equipped with an air conditioning system integrated with RCP, has reported occupant thermal discomfort. Experimental data were used to calibrate a building model and dynamic building energy simulations were carried out to analyse indoor air temperature, relative humidity, predicted mean vote (PMV) and predicted percentage of dissatisfied (PPD) indexes under different inlet air temperatures, to identify the best design conditions for energy efficiency and thermal comfort improvement. It was found that the highest advantages can be obtained when neutral air is supplied.