bluuebunny/crossref_metadata_2025 huggingface dataset:
Host plant-mediated effects of elevated CO2 and temperature on growth and developmental parameters of Zygogramma bicolorata (Coleoptera: Chrysomelidae)
Authors: Kumar Lavkush , Sushilkumar  , Choudhary Jaipal Singh , Kumar Bhumesh
Published: July 2020

Mexican beetle, Zygogramma bicolorata Pallister (Coleptera: Chrysomelidae) is a potential weed control biocontrol agent in Australia, India and other countries. Its grubs and adults feed on the leaves of parthenium weed, Parthenium hysterophorus and check the further growth of the plant. Experiments were conducted to understand host plant-mediated effects of elevated temperature and elevated CO2 on biocontrol agent Z. bicolorata. Food consumption, utilization, ecological efficiency and life-table parameters of Z. bicolorata were studied in grubs and adults stage up to diapause. Reduction of leaf nitrogen in parthenium weed foliage with a significant increase in carbon and C:N ratio was recorded at elevated CO2. Elevated CO2 and temperature had no effect on adult longevity before diapausing. Duration of egg's hatching, specific stages of grub and pupa of Z. bicolorata were significantly longer when beetles fed on leaves grown under elevated CO2 but these parameters decreased significantly on leaves grown under elevated temperature. Significantly high consumption rates with low growth and digestion conversions were observed under elevated CO2 and/or in coupled with elevated temperature. Elevated CO2 and temperature-grown parthenium weed foliage also had a significant effect on Z. bicolorata intrinsic rate of increase (R), finite rate of increase (λ), mean generation time (T), and gross reproductive rate. Changed quality of parthenium weed leaves in elevated CO2 and temperature levels resulted in the increase of consumption, slower food conversion rates, increase in developmental period with reduced reproduction efficiency of Z. bicolorata. Our results indicate that the reproduction efficiency of Z. bicolorata is likely to be reduced as the climate changes, despite increased feeding rates exhibited by grubs and adult beetles on parthenium weed foliage.
Brachiaria and Panicum maximum in an integrated crop–livestock system and a second-crop maize system in succession with soybean
Authors: Dias Mariana Borges de Castro , Costa Kátia Aparecida de Pinho , Severiano Eduardo da Costa , Bilego Ubirajara Oliveira , Neto Antonio Eduardo Furtini , Almeida Dieimisson Paulo , Brand Simone Cristiane , Vilela Lourival
Published: July 2020

Owing to its contribution to the maintenance of carbon stocks, soil nitrogen and nutrient cycling for subsequent crops, the integrated systems become increasingly important for agricultural conservation. Thus, the objective of this study was to evaluate the biomass production of and total nutrient in Brachiaria spp. and Panicum maximum forage grasses used as mulch and soybean yields in an integrated crop–livestock system and second-crop maize succession system. The treatments consisted of the following cropping systems: Xaraes palisadegrass intercropped with soybean, Congo grass intercropped with soybean, Mombaça guinea grass intercropped with soybean, Tamani guinea grass intercropped with soybean and a soybean/maize succession system. The forage grasses were established during the soybean R6–R7 stage. Compared with Congo grass, Xaraes palisadegrass, Mombaça guinea grass and Tamani guinea grass produced more biomass and equivalent amounts of fertilizer returned to the soil and resulted in greater nutrient cycling, indicating the benefits of these grasses for use as mulch in integrated production systems. Maize had a greater C/N ratio, but the forage grasses also exhibited high potential by protecting the soil until the end of the soybean development cycle. The use of an integrated crop–livestock system combined with a forage cropping system provided greater soil nutrient cycling than the maize cropping system did, which resulted in increased soybean yields, thus contributing to the sustainability of agricultural systems.
Identification of pathogens causing invasive fungal rhinosinusitis in surgical biopsies using polymerase chain reaction
Authors: Chaturantabut S , Kitkumthorn N , Mutirangura A , Praditphol N , Chindamporn A , Thorner P S , Keelawat S
Published: July 2020

Background

Invasive fungal rhinosinusitis is associated with high morbidity and mortality. Rapid pathogen identification is mandatory, but fresh tissue is not always available. A polymerase chain reaction method was designed in order to detect fungi in formalin-fixed paraffin-embedded samples. This was applied to a retrospective series of tissue biopsies from Thai patients with invasive fungal rhinosinusitis.

Methods

Tissue blocks from 64 cases yielded adequate DNA. Three sequential polymerase chain reaction were performed: ZP3 (housekeeping gene) and panfungal polymerase chain reactions, and a differentiating polymerase chain reaction based on the 5.8s ribosomal RNA and internal transcribed spacer 2 regions. The polymerase chain reaction products were then sequenced.

Results

Polymerase chain reaction identified a fungal pathogen in 20 of 64 cases (31 per cent). Aspergillus species was the most common cause of invasive fungal rhinosinusitis (nine cases). Other causes included candida (n = 4), cladosporium (n = 4), mucor (n = 1), alternaria (n = 1) and dendryphiella (n = 1) species.

Conclusion

Polymerase chain reaction can provide rapid identification of fungal pathogens in paraffin-embedded tissue, enabling prompt treatment of invasive fungal rhinosinusitis.
Ten-year course of cognition in first-episode non-affective psychosis patients: PAFIP cohort
Authors: Rodríguez-Sánchez José Manuel , Setién-Suero Esther , Suárez-Pinilla Paula , Mayoral Van Son Jaqueline , Vázquez-Bourgon Javier , Gil López Patxi , Crespo-Facorro Benedicto , Ayesa-Arriola Rosa ,   PAFIP group
Published: July 2020

Background

A large body of research states that cognitive impairment in schizophrenia is static. Nevertheless, most previous studies lack a control group or have small study samples or short follow-up periods.

Method

We aimed to address these limitations by studying a large epidemiological cohort of patients with first-episode schizophrenia spectrum disorders and a comparable control sample for a 10-year period.

Results

Our results support the generalized stability of cognitive functions in schizophrenia spectrum disorders considering the entire group. However, the existence of a subgroup of patients characterized by deteriorating cognition and worse long-term clinical outcomes must be noted. Nevertheless, it was not possible to identify concomitant factors or predictors of deterioration (all Ps > 0.05).

Conclusions

Cognitive functions in schizophrenia spectrum disorder are stable; however, a subgroup of subjects that deteriorate can be characterized.
Associations of physical and psychiatric conditions with chronic fatigue syndrome in Germany: an exploratory case-control study
Authors: Jacob Louis , Haro Josep Maria , Kostev Karel
Published: July 2020

Background

Only a few studies have analyzed the effects of physical and psychiatric conditions on the risk of chronic fatigue syndrome (CFS). Therefore, the goal of this exploratory case-control study was to investigate the associations of physical and psychiatric conditions with CFS in almost 19 800 adults from Germany.

Methods

This study included patients diagnosed for the first time with CFS in one of 1238 general practices in Germany between 2010 and 2017 (index date). Controls without CFS were matched (1:1) to cases with CFS by sex, age, index year, and practice. Physical and psychiatric conditions diagnosed in the year prior to the index date were included if they were present in at least 3% of patients with CFS. Associations between physical and psychiatric conditions (33 potential independent variables) and CFS (dependent variable) were analyzed in an adjusted conditional logistic regression model, and physical and psychiatric disorders were included in the model using forward stepwise selection.

Results

This study included 9896 cases with CFS and 9896 controls without CFS [65.1% women; mean (standard deviation) age 49.5 (18.3) years]. Seven conditions were associated with CFS in the adjusted regression model. The disorders displaying the strongest relationship with CFS were cancer [odds ratio (OR) = 2.57, 95% confidence interval (CI) = 2.24–2.95], sleep disorders (OR = 1.88, 95% CI = 1.66–2.12) and depression (OR = 1.77, 95% CI = 1.61–1.95).

Conclusions

Cancer, sleep disorders, and depression were strongly and positively associated with CFS. Additional studies are needed to gain a better understanding of the mechanisms underlying these relationships.
The prosody of other-repetition in Italian: A system of tunes
Authors: Rossi Giovanni
Published: July 2020

As part of the project reported on in this special issue, the present study provides an overview of the types of action accomplished by other-repetition in Italian, with particular reference to the variety of the language spoken in the northeastern province of Trento. The analysis surveys actions within the domain of initiating repair, actions that extend beyond initiating repair, and actions that are alternative to initiating repair. Pitch contour emerges as a central design feature of other-repetition in Italian, with six nuclear contours associated with distinct types of action, sequential trajectories, and response patterns. The study also documents the interplay of pitch contour with other prosodic features (pitch span and register) and visible behavior (head nods, eyebrow movements). (Repetition, conversation, prosody, intonation, action, Italian)*
Stable Robotic Grasping of Multiple Objects using Deep Neural Networks
Authors: Kim Dongeon , Li Ailing , Lee Jangmyung
Published: July 2020

Optimal grasping points for a robotic gripper were derived, based on object and hand geometry, using deep neural networks (DNNs). The optimal grasping cost functions were derived using probability density functions for each local cost function of the normal distribution. Using the DNN, the optimum height and width were set for the robot hand to grasp objects, whose geometric and mass centre points were also considered in obtaining the optimum grasping positions for the robot fingers and the object. The proposed algorithm was tested on 10 differently shaped objects and showed improved grip performance compared to conventional methods.
Transcatheter ablation using near-zero fluoroscopy in children with focal atrial tachycardia: a single-centre experience
Authors: Balli Sevket , Kucuk Mehmet
Published: July 2020

Objective:

Focal atrial tachycardia accounts for up to 10–15% of supraventricular tachycardiasubstrates in patients < 30 years. In this study, we aimed to demonstrate the outcome of transcatheter ablation procedures performed through three-dimensional electroanatomic mapping systems using minimal fluoroscopy in a paediatric cohort with focal atrial tachycardia.

Methods:

Forty-nine consecutive patients with focal atrial tachycardia who underwent an electrophysiologic study and a transcatheter ablation procedure in our hospital from September 2014 to February 2020 were included into the study.

Results:

The mean weight of the patients was 48.63 ± 15.4 kg, and the mean age was 14.56 ± 3.5 (5.5–18.4) years. The tachycardia was defined as incessant in 26 patients. Thirteen patients had left ventricular systolic dysfunction with a mean left ventricular ejection fraction of 38.47 ± 12.4% on echocardiography. The mean procedure time was 148.7 ± 94.5 minutes. Transseptal puncture and thus fluoroscopy were required in nine patients. The mean fluoroscopy time was 4.51 ± 5.9 minutes. No fluoroscopy was needed in ablations performed in the right atrium. The acute success rate of the ablation procedures was 97.9%. The mean follow-up period was 50.71 ± 23.5 months. Recurrence was noted in two patients (4.2%).

Conclusion:

The outcomes of three-dimensional electroanatomic mapping-guided transcatheter ablation procedures are promising with high acute success, low recurrence and complication rates in children with focal atrial tachycardia. The use of fluoroscopy can be significantly decreased with three-dimensional mapping systems in this group of patients.
Effect of maternal and postnatal cocoa supplementation on testicles of adult Wistar rats
Authors: Lima Juliana , Cardoso Letícia , Rocha Gabrielle , Fernandes-Santos Caroline , de Souza Diogo , Sampaio Francisco Jose , Gregorio Bianca
Published: July 2020

Early weaning can lead to changes in the morphology of organs in adulthood, and the consumption of functional foods during lactation and postnatal life is believed to prevent these changes. However, it is not known if early weaning affects testicular morphology and if the use of cocoa can prevent that. We studied the effects of maternal and postnatal supplementation of cocoa powder on the testicular morphology of early weaned adult rats. The animals were divided into four groups (n = 6 each), control group, cocoa control group, early weaning (EW) group, and cocoa early weaning (EWCa) group, and were analyzed for 90 d, after which they were euthanized. The animals from the EW group showed a reduction in the tubular diameter and height of the seminiferous epithelium, a decrease in epithelial surface density (Sv), and an increase in the lumen and proper tunic. However, the animals from the EWCa group showed an increase in the diameter and height of the epithelium, an increase in the epithelium Sv, and a decrease in the lumen and the proper tunic. The early weaning promotes morphological changes in the testicles; however, supplementation with cocoa powder can preserve the testicular histoarchitecture.
Human rights courts and global constitutionalism: Coordination through judicial dialogue
Authors: SANDHOLTZ WAYNE
Published: July 2020

International courts regularly cite each other, partly as a means of building legitimacy. This study aims to show that judicial dialogue among the regional human rights courts and the Human Rights Committee has an additional effect: it contributes to the construction of a rights-based global constitutionalism. Judicial dialogue among the human rights courts is purposeful because the courts see themselves as embedded in, and contributing to, a global human rights legal system. Cross-citation among the human rights courts advances the construction of rights-based global constitutionalism in that it provides a basic degree of coordination among the regional courts. The jurisprudence of the United Nations Human Rights Committee (HRC), as an authoritative interpreter of core international human rights norms, plays the role of a central focal point for the decentralized coordination of jurisprudence. Using original data, this study demonstrates the extent of citations among the regional human rights courts and from them to the HRC. The network of regional courts and the HRC is building an emergent institutional structure for global rights-based constitutionalism.
Emotional Dissonance in the Spanish Services Sector: The Role of Support in the Workplace
Authors: Sora Beatriz , Vera María
Published: July 2020

The purpose of this study was twofold. The first aim was to analyze the detrimental effect that emotional dissonance may have on service workers by testing its relationship with job satisfaction, intention to leave the organization, and organizational deviance. The second was to test whether two types of social support (i.e., co-worker and organizational support) and their combination moderate these relationships from a multilevel perspective. The sample was composed of 556 employees from Spanish service sector. Using random coefficient models analyses, results showed, first, that emotional dissonance was related to lower levels of job satisfaction (PE =–.1,p <.05) and higher levels of intention to leave the organization (PE =.12,p <.05); second, that co-worker support moderate the relationship between emotional dissonance and job satisfaction (PE =.10,p <.05), organizational deviance (PE =–.08,p <.05), and intention to leave the organization (PE =–.13,p <.05); third, organizational support, conceptualized as a collective construct at organizational level, moderate the relationship between emotional dissonance and organizational deviance (PE= –.08,p< .05); and finally, the combination of both types of support do not explained additional variance of the emotional dissonance-outcomes relation. These results underline the need to take into consideration different source of social support and their levels of analysis to better understand emotional dissonance and its outcomes.
Weed control practices and awareness of herbicide resistance among cereal farmers of northern Greece
Authors: Toubou Elisavet , Vindena Vassiliki , Damalas Christos A. , Koutroubas Spyridon D.
Published: July 2020

Knowledge of weed control practices and farmers’ awareness of herbicide resistance could be a basis for improving weed management programs with respect to herbicide resistance, but research on this topic is limited. This study reports current weed control practices and levels of awareness of herbicide resistance among cereal farmers of northern Greece. Face-to-face interviews were conducted with 250 cereal farmers of Evros district, based on a structured questionnaire. Most farmers (82.8%) used herbicides in cereal production, with one application per growing season. Farmers appeared divided with respect to using the same herbicide each year; the majority of the farmers (90.8%) applied crop rotation. Almost half of the farmers (47.2%) did not know what herbicide resistance is, but most farmers (75.1%) felt herbicide resistance would be a problem for them. According to their answers on nine knowledge questions about herbicide resistance, 66.8% of the farmers had good knowledge, and 33.2% had poor knowledge. Almost seven in 10 farmers (69.8%) did not consider herbicide resistance when purchasing an herbicide for use, and only 40.4% were willing to change common weed control practices to prevent herbicide resistance. Awareness of herbicide resistance did not differ by sex; poor awareness levels increased with advanced age, low education levels, and small farm size. Farmers who used chemical weed control had higher awareness levels of herbicide resistance than farmers who never used herbicides. Farmers who were keeping records of herbicide applications, those who observed low efficacy of herbicides in their field, and those who applied crop rotation had high awareness levels of herbicide resistance, whereas farmers who used the same herbicide each year had poor awareness. Findings shed light on inter-relationships between farmers’ awareness of herbicide resistance and current weed control practices that could be useful for targeted extension education.
Quantifying Regional Surface Energy Responses to Forest Structural Change in Nordic Fennoscandia
Authors: Kumkar Yogesh , Astrup Rasmus , Stordal Frode , Bright Ryan M.
Published: July 2020

In a climate model, surface energy and water fluxes of the vegetated ecosystem largely depend on important structural attributes like leaf area index and canopy height. For forests, management can greatly alter these attributes with resulting consequences for the surface albedo, surface roughness, and evapotranspiration. The sensitivity of surface energy and water budgets to alterations in forest structure is relatively unknown in boreal regions, particularly in Nordic Fennoscandia (Norway, Sweden, and Finland), where the forest management footprint is large. Here we perform offline simulations to quantify the sensitivity of surface heat and moisture fluxes to changes in forest composition and structure across daily, seasonal, and annual time scales. For the region on average, it is found that broadleaved deciduous forests cool the surface by 0.16 K annually and 0.3 K in the growing season owed to higher year‐round albedo and lower Bowen ratio, yet in some locations the local cooling can be as much as 2.4 K and 3.0 K, respectively. Moreover, fully developed forests cool the surface by 0.04 K annually in our domain owed to higher evapotranspiration, reaching up to 0.4 K locally in some locations, whereas undeveloped forests warm annually by 0.14 K owed to much lower evapotranspiration reaching up to 0.8 K for some locations. If regional forests are ever to be managed for the local climate regulation services that they provide, our results are an important first step illuminating the potential adverse impacts or benefits across space and time.
Stress Field Variation During the 2019 Ridgecrest Earthquake Sequence
Authors: Sheng Shuzhong , Meng Lingsen
Published: July 2020

We investigate the spatiotemporal variations of the stress field and the deviatoric stress associated with the 2019 Ridgecrest earthquake sequence using the USGS earthquake focal mechanisms. We find that while the M 6.4 foreshock causes no notable stress changes, the stress field adjustment due to the M 7.1 mainshock exhibits significant spatial complexities. The area of large stress change is generally correlated with the fault segments with large coseismic slip. Although there are no resolvable stress changes at the two terminal ends of the mainshock, the mainshock produces a ~5° clockwise rotation of the maximum horizontal stress (SHmax) near the M 6.4 foreshock epicenter. The inferred deviatoric stress is approximately 8.0 MPa, almost equivalent to the coseismic stress drop of 7.4 MPa in its epicenter area. Such nearly complete stress release is supported by the postseismic stress heterogeneity near the mainshock epicenter.
Daytime Dynamo Electrodynamics With Spiral Currents Driven by Strong Winds Revealed by Vapor Trails and Sounding Rocket Probes
Authors: Pfaff R. , Larsen M. , Abe T. , Habu H. , Clemmons J. , Freudenreich H. , Rowland D. , Bullett T. , Yamamoto M.‐Y. , Watanabe S. , Kakinami Y. , Yokoyama T. , Mabie J. , Klenzing J. , Bishop R. , Walterscheid R. , Yamamoto M. , Yamazaki Y. , Murphy N. , Angelopoulos V.
Published: July 2020

We investigate the forces and atmosphere‐ionosphere coupling that create atmospheric dynamo currents using two rockets launched nearly simultaneously on 4 July 2013 from Wallops Island (USA), during daytime Sq conditions with ΔH of −30 nT. One rocket released a vapor trail observed from an airplane which showed peak velocities of >160 m/s near 108 km and turbulence coincident with strong unstable shear. Electric and magnetic fields and plasma density were measured on a second rocket. The current density peaked near 110 km exhibiting a spiral pattern with altitude that mirrored that of the winds, suggesting the dynamo is driven by tidal forcing. Such stratified currents are obscured in integrated ground measurements. Large electric fields produced a current opposite to that driven by the wind, believed created to minimize the current divergence. Using the observations, we solve the dynamo equation versus altitude, providing a new perspective on the complex nature of the atmospheric dynamo.
Destruction of Cratonic Lithosphere Induced by Oceanic Subduction Initiation
Authors: Chen Miao , Shen Xiaobing , Leng Wei , Chen Ling
Published: July 2020

Based on the plate reconstruction which shows that most core parts of present cratons locate in the interior of the continents during the supercontinent cycles, we propose that the oceanic subduction initiation may trigger the destruction of marginal cratonic lithosphere. We develop a simplified force analysis for the destruction of subcontinental lithospheric mantle (SCLM) by the subduction initiation. Our analysis suggests three key parameters: the SCLM thickness, the contact angle between SCLM and oceanic plate, and the coefficient of friction of the SCLM. Our numerical models further support our analysis and demonstrate that a thicker SCLM with a larger contact angle is easier to be destructed by subduction initiation, and the yield strength of the SCLM has a significant impact on the spatial range of its destruction. Our results provide important insights into the parameters controlling cratonic lithospheric destruction and explain the distribution of preserved cratons in the interior of continental plates.
The Stream Length Duration Curve: A Tool for Characterizing the Time Variability of the Flowing Stream Length
Authors: Botter G. , Durighetto N.
Published: July 2020

In spite of the importance of stream network dynamics for hydrology, ecology, and biogeochemistry, there is limited availability of analytical tools suitable for characterizing the temporal variability of the active fraction of river networks. To fill this gap, we introduce the concept of Stream Length Duration Curve (SLDC), the inverse of the exceedance probability of the total length of active streams. SLDCs summarize efficiently the effect of hydrological variability on the length of the flowing streams under a variety of settings. A set of stochastic network models is developed to link the features of the local hydrological status of the network nodes with the shape of the SLDC. We show that the mean network length is dictated by the mean persistency of the nodes, whereas the shape of the SLDC is driven by the spatial distribution of the local persistencies and their network‐scale spatial correlation. Ten field surveys performed in 2018 were used to estimate the empirical SLDC of the Valfredda river (Italy), which was found to be steep and regular—indicating a pronounced sensitivity of the active stream length to the underlying hydrological conditions. Available observations also suggest that the activation of temporary reaches during network expansion is hierarchical, from the most to the least persistent stretches. Under these circumstances, the SLDC corresponds to the spatial Cumulative Distribution Function of the nodes persistencies. The study provides a sound theoretical basis for the analyses of network dynamics in temporary rivers.
Secondary findings in inherited heart conditions: a genotype-first feasibility study to assess phenotype, behavioural and psychosocial outcomes
Authors: Ormondroyd Elizabeth , Harper Andrew R. , Thomson Kate L. , Mackley Michael P. , Martin Jennifer , Penkett Christopher J. , Salatino Silvia , Stark Hannah , Stephens Jonathan , Watkins Hugh
Published: July 2020

Disclosing secondary findings (SF) from genome sequencing (GS) can alert carriers to disease risk. However, evidence around variant-disease association and consequences of disclosure for individuals and healthcare services is limited. We report on the feasibility of an approach to identification of SF in inherited cardiac conditions (ICC) genes in participants in a rare disease GS study, followed by targeted clinical evaluation. Qualitative methods were used to explore behavioural and psychosocial consequences of disclosure. ICC genes were analysed in genome sequence data from 7203 research participants; a two-stage approach was used to recruit genotype-blind variant carriers and matched controls. Cardiac-focused medical and family history collection and genetic counselling were followed by standard clinical tests, blinded to genotype. Pathogenic ICC variants were identified in 0.61% of individuals; 20 were eligible for the present study. Four variant carriers and seven non-carrier controls participated. One variant carrier had a family history of ICC and was clinically affected; a second was clinically unaffected and had no relevant family history. One variant, in two unrelated participants, was subsequently reclassified as being of uncertain significance. Analysis of qualitative data highlights participant satisfaction with approach, willingness to follow clinical recommendations, but variable outcomes of relatives’ engagement with healthcare services. In conclusion, when offered access to SF, many people choose not to pursue them. For others, disclosure of ICC SF in a specialist setting is valued and of likely clinical utility, and can be expected to identify individuals with, and without a phenotype.
Coronin 1C inhibits melanoma metastasis through regulation of MT1-MMP-containing extracellular vesicle secretion
Authors: Tagliatela Alicia C. , Hempstead Stephanie C. , Hibshman Priya S. , Hockenberry Max A. , Brighton Hailey E. , Pecot Chad V. , Bear James E.
Published: July 2020

Coronin 1C is overexpressed in multiple tumors, leading to the widely held view that this gene drives tumor progression, but this hypothesis has not been rigorously tested in melanoma. Here, we combined a conditional knockout of Coronin 1C with a genetically engineered mouse model of PTEN/BRAF-driven melanoma. Loss of Coronin 1C in this model increases both primary tumor growth rates and distant metastases. Coronin 1C-null cells isolated from this model are more invasive in vitro and produce more metastatic lesions in orthotopic transplants than Coronin 1C-reexpressing cells due to the shedding of extracellular vesicles (EVs) containing MT1-MMP. Interestingly, these vesicles contain melanosome markers suggesting a melanoma-specific mechanism of EV release, regulated by Coronin 1C, that contributes to the high rates of metastasis in melanoma.
Stimulus modality influences session-to-session transfer of training effects in auditory and tactile streaming-based P300 brain–computer interfaces
Authors: Ziebell P. , Stümpfig J. , Eidel M. , Kleih S. C. , Kübler A. , Latoschik M. E. , Halder S.
Published: July 2020

Despite recent successes, patients suffering from locked-in syndrome (LIS) still struggle to communicate using vision-independent brain–computer interfaces (BCIs). In this study, we compared auditory and tactile BCIs, regarding training effects and cross-stimulus-modality transfer effects, when switching between stimulus modalities. We utilized a streaming-based P300 BCI, which was developed as a low workload approach to prevent potential BCI-inefficiency. We randomly assigned 20 healthy participants to two groups. The participants received three sessions of training either using an auditory BCI or using a tactile BCI. In an additional fourth session, BCI versions were switched to explore possible cross-stimulus-modality transfer effects. Both BCI versions could be operated successfully in the first session by the majority of the participants, with the tactile BCI being experienced as more intuitive. Significant training effects were found mostly in the auditory BCI group and strong evidence for a cross-stimulus-modality transfer occurred for the auditory training group that switched to the tactile version but not vice versa. All participants were able to control at least one BCI version, suggesting that the investigated paradigms are generally feasible and merit further research into their applicability with LIS end-users. Individual preferences regarding stimulus modality should be considered.
Did expanded access to denture services improve chewing ability in the Korean older population? Results of a regression discontinuity analysis
Authors: Kim Nam-Hee , Kawachi Ichiro
Published: July 2020

The Korean National Health Insurance expanded the dental insurance in 2012 to cover denture services for older adults. We analyzed whether the new policy improved of chewing ability in the eligible population. We used regression discontinuity (RD), a quasi-experimental design, to analyze the effects of the expanded dental insurance. We analyzed data from the Korea National Health and Nutrition Examination Survey conducted in 2010 and 2015. The study population consisted of two groups: the treatment group, aged 65 and above who were eligible; and the control group, under 65 years of age who were not eligible for the dental insurance benefit. The main outcome evaluated was self-reported chewing difficulty. The RD analysis showed that in 2015, the chewing difficulty in aged above 65 was 2.2% lower than in those aged under 65. However, the difference was not statistically significant (P = 0.76). The results from the falsification testing of predetermined covariates, placebo cut-offs, and bandwidths validated our main conclusion. The expansion of dental insurance benefits to include dentures for the older adults did not improve the chewing ability in the eligible population. Future studies should evaluate long-term outcomes of oral health as well as the social impacts on the elderly.
Direct identification of bacterial and human proteins from infected wounds in living 3D skin models
Authors: Havlikova Jana , May Robin C. , Styles Iain B. , Cooper Helen J.
Published: July 2020

Trauma is one of the leading causes of death in people under the age of 49 and complications due to wound infection are the primary cause of death in the first few days after injury. The ESKAPE pathogens are a group of bacteria that are a leading cause of hospital-acquired infections and a major concern in terms of antibiotic resistance. Here, we demonstrate a novel and highly accurate approach for the rapid identification of ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) directly from infected wounds in 3D in vitro skin models. Wounded skin models were inoculated with bacteria and left to incubate. Bacterial proteins were identified within minutes, directly from the wound, by liquid extraction surface analysis mass spectrometry. This approach was able to distinguish closely related strains and, unlike genomic approaches, can be modified to provide dynamic information about pathogen behaviour at the wound site. In addition, since human skin proteins were also identified, this method offers the opportunity to analyse both host and pathogen biomarkers during wound infection in near real-time.
On the evolution of sexual receptivity in female primates
Authors: Rooker Kelly , Gavrilets Sergey
Published: July 2020

There has been much interest in the evolutionary forces responsible for, and underlying the diversity in, female primate reproductive cycles. While there has been limited research on sexual receptivity in primates, this has been one recurring topic of interest. Some primate species are like humans, sexually receptive to mating throughout their entire estrus cycle, while other species are the opposite, receptive for mere hours out of their several-week cycles. Why is there such prominent variation in sexual receptivity length among primate species? Here we examine the evolutionary trade-offs associated with sexual receptivity length using mathematical modeling. We investigate how various factors, including having ovulation signs present versus concealed ovulation, female physiological costs, and group size, each influence the length of females’ receptive periods. We find that both continuous receptivity and very short lengths of receptivity are able to evolve. Our model predicts that increasing the impacts of infanticide will increase the length of the female receptive period, emphasizing the possible importance of paternity confusion. Similar effects can also be achieved by increasing the non-genetic benefits provided by males. Overall, our work offers a theoretical framework for understanding the evolution and diversity of mating traits in female primates.
Increased predominance of the matured ventricular subtype in embryonic stem cell-derived cardiomyocytes in vivo
Authors: Ichimura Hajime , Kadota Shin , Kashihara Toshihide , Yamada Mitsuhiko , Ito Kuniaki , Kobayashi Hideki , Tanaka Yuki , Shiba Naoko , Chuma Shinichiro , Tohyama Shugo , Seto Tatsuichiro , Okada Kenji , Kuwahara Koichiro , Shiba Yuji
Published: July 2020

Accumulating evidence suggests that human pluripotent stem cell-derived cardiomyocytes can affect “heart regeneration”, replacing injured cardiac scar tissue with concomitant electrical integration. However, electrically coupled graft cardiomyocytes were found to innately induce transient post-transplant ventricular tachycardia in recent large animal model transplantation studies. We hypothesised that these phenomena were derived from alterations in the grafted cardiomyocyte characteristics. In vitro experiments showed that human embryonic stem cell-derived cardiomyocytes (hESC-CMs) contain nodal-like cardiomyocytes that spontaneously contract faster than working-type cardiomyocytes. When transplanted into athymic rat hearts, proliferative capacity was lower for nodal-like than working-type cardiomyocytes with grafted cardiomyocytes eventually comprising only relatively matured ventricular cardiomyocytes. RNA-sequencing of engrafted hESC-CMs confirmed the increased expression of matured ventricular cardiomyocyte-related genes, and simultaneous decreased expression of nodal cardiomyocyte-related genes. Temporal engraftment of electrical excitable nodal-like cardiomyocytes may thus explain the transient incidence of post-transplant ventricular tachycardia, although further large animal model studies will be required to control post-transplant arrhythmia.
Synergistic adsorption-photocatalytic degradation effect and norfloxacin mechanism of ZnO/ZnS@BC under UV-light irradiation
Authors: Liu Wen , He Tianpei , Wang Yonghong , Ning Ge , Xu Zhenggang , Chen Xiaoyong , Hu Xinjiang , Wu Yaohui , Zhao Yunlin
Published: July 2020

Norfloxacin (NOF) is an environmentally harmful and ubiquitous aquatic pollutant with extensive production and application. In this study, a novel composition named carbon-based composite photocatalytic material of zinc oxide and zinc sulphide (ZnO/ZnS@BC) was successfully obtained by the impregnation-roasting method to remove NOF under UV-light. Scanning electron microscopy, X-ray photoelectron spectroscopy, transmission electron microscopy and energy dispersive spectrometer characterised the composition. ZnO/ZnS was successfully decorated on the surface of biochar (BC). The pH, the ZnSO4/PS ratio, and ions and quenchers, were investigated. High removal efficiency was obtained with a pH of 7 and a ZnSO4/PS ratio of 1:1, and the removal ratio of NOF reached 95% within three hours; the adsorption and degradation ratios reached 46% and 49%, respectively. Fe2+ promoted the degradation of NOF, whereas other ions inhibited it, with NO3− showing the strongest inhibitory effect. Three reactive species (tert-butanol, quinone, and ammonium oxala) were identified in the catalytic system. The decreasing order of the contribution of each reactive species was: O2− > ·OH− > h+. Additionally, a recycling experiment demonstrated the stability of the catalyst; the catalytic degradation ratio of NOF reached 78% after five successive runs. Therefore, ZnO/ZnS@BC possessed strong adsorption capacity and high ultraviolet photocatalysis ability.
A comparison of proteomic, genomic, and osteological methods of archaeological sex estimation
Authors: Buonasera Tammy , Eerkens Jelmer , de Flamingh Alida , Engbring Laurel , Yip Julia , Li Hongjie , Haas Randall , DiGiuseppe Diane , Grant Dave , Salemi Michelle , Nijmeh Charlene , Arellano Monica , Leventhal Alan , Phinney Brett , Byrd Brian F. , Malhi Ripan S. , Parker Glendon
Published: July 2020

Sex estimation of skeletons is fundamental to many archaeological studies. Currently, three approaches are available to estimate sex–osteology, genomics, or proteomics, but little is known about the relative reliability of these methods in applied settings. We present matching osteological, shotgun-genomic, and proteomic data to estimate the sex of 55 individuals, each with an independent radiocarbon date between 2,440 and 100 cal BP, from two ancestral Ohlone sites in Central California. Sex estimation was possible in 100% of this burial sample using proteomics, in 91% using genomics, and in 51% using osteology. Agreement between the methods was high, however conflicts did occur. Genomic sex estimates were 100% consistent with proteomic and osteological estimates when DNA reads were above 100,000 total sequences. However, more than half the samples had DNA read numbers below this threshold, producing high rates of conflict with osteological and proteomic data where nine out of twenty conditional DNA sex estimates conflicted with proteomics. While the DNA signal decreased by an order of magnitude in the older burial samples, there was no decrease in proteomic signal. We conclude that proteomics provides an important complement to osteological and shotgun-genomic sex estimation.
An antibody panel for highly specific detection and differentiation of Zika virus
Authors: Kabir Md Alamgir , Soto-Acosta Ruben , Sharma Sandhya , Bradrick Shelton S. , Garcia-Blanco Mariano A. , Caputi Massimo , Asghar Waseem
Published: July 2020

Zika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitos. ZIKV can be transmitted from mother to fetus during pregnancy and can cause microcephaly and other birth defects. Effective vaccines for Zika are yet to be approved. Detection of the ZIKV is based on serological testing that often shows cross-reactivity with the Dengue virus (DENV) and other flaviviruses. We aimed to assemble a highly specific anti-Zika antibody panel to be utilized in the development of a highly specific and cost-effective ZIKV rapid quantification assay for viral load monitoring at point-of-care settings. To this end, we tested the affinity and specificity of twenty one commercially available monoclonal and polyclonal antibodies against ZIKV and DENV envelope proteins utilizing nine ZIKV and twelve DENV strains. We finalized and tested a panel of five antibodies for the specific detection and differentiation of ZIKV and DENV infected samples.
The role of chronotype in the interaction between the alerting and the executive control networks
Authors: Martínez-Pérez Víctor , Palmero Lucía B. , Campoy Guillermo , Fuentes Luis J.
Published: July 2020

Chronotype refers to the time of day preferred by individuals to perform daily activities according to their circadian rhythm. We asked whether synchrony effects, that is, the difference in performance between the optimal and non-optimal time of day as a function of chronotype, are observed in two tasks that differently involve the endogenous component of the alerting network, the psychomotor visual task (PVT) and the flanker task. From an initial sample of 132 students that filled in the Morningness–Eveningness Questionnaire (MEQ), 18 were classified as Morning-types and 16 as Evening-types. Evening-types showed synchrony effects in both tasks, whereas Morning-types failed to show synchrony effects in the flanker task and when the PVT was first performed at the non-optimal time of day. Thus, Morning-types might have seen increased their vigilant attention at their non-optimal time of day due to the cognitive demands of the flanker task and to the novelty with the PVT. Phasic alerting generated by alerting tones increased conflict score in the flanker task, but time of day did not modulate the congruence effect. Chronotype determines vigilant attention more decisively in Evening-types than in Morning-types individuals. Also, exogenous but not endogenous alerting exerts a deleterious effect on conflict resolution.
Conjuncted photo-thermoelectric effect in ZnO–graphene nanocomposite foam for self-powered simultaneous temperature and light sensing
Authors: Zhao Huiqi , Ouyang Bangsen , Han Lu , Mishra Yogendra Kumar , Zhang Zhiqiang , Yang Ya
Published: July 2020

The self-powered sensors are more and more important in current society. However, detecting both light and temperature signals simultaneously without energy waste and signal interference is still a challenge. Here, we report a ZnO/graphene nanocomposite foam-based self-powered sensor, which can realize the simultaneous detection of light and temperature by using the conjuncted photo-thermoelectric effect in ZnO–graphene nanocomposite foam sensor. The output current under light, heating and cooling of the device with the best ZnO/graphene ratio (8:1) for the foam can reach 1.75 µA, 1.02 µA and 0.70 µA, respectively, which are approximately three fold higher than them of devices with other ZnO/graphene ratios. The ZnO–graphene nanocomposite foam device also possesses excellent thermoelectric and photoelectric performances for conjuncted lighting and heating detection without mutual interference. The ZnO–graphene nanocomposite foam device exhibits a new designation on the road towards the fabrication of low cost and one-circuit-based multifunction sensors and systems.
Molecular characterization and gene expression modulation of the alternative oxidase in a scuticociliate parasite by hypoxia and mitochondrial respiration inhibitors
Authors: Folgueira Iría , Lamas Jesús , Sueiro Rosa Ana , Leiro José Manuel
Published: July 2020

Philasterides dicentrarchi is a marine benthic microaerophilic scuticociliate and an opportunistic endoparasite that can infect and cause high mortalities in cultured turbot (Scophthalmus maximus). In addition to a cytochrome pathway (CP), the ciliate can use a cyanide-insensitive respiratory pathway, which indicates the existence of an alternative oxidase (AOX) in the mitochondrion. Although AOX activity has been described in P. dicentrarchi, based on functional assay results, genetic evidence of the presence of AOX in the ciliate has not previously been reported. In this study, we conducted genomic and transcriptomic analysis of the ciliate and identified the AOX gene and its corresponding mRNA. The AOX gene (size 1,106 bp) contains four exons and three introns that generate an open reading frame of 915 bp and a protein with a predicted molecular weight of 35.6 kDa. The amino acid (aa) sequence of the AOX includes an import signal peptide targeting the mitochondria and the protein is associated with the inner membrane of the mitochondria. Bioinformatic analysis predicted that the peptide is a homodimeric glycoprotein, although monomeric forms may also appear under native conditions, with EXXH motifs associated with the diiron active centers. The aa sequences of the AOX of different P. dicentrarchi isolates are highly conserved and phylogenetically closely related to AOXs of other ciliate species, especially scuticociliates. AOX expression increased significantly during infection in the host and after the addition of CP inhibitors. This confirms the important physiological roles of AOX in respiration under conditions of low levels of O2 and in protecting against oxidative stress generated during infection in the host.
Fe-functionalized paramagnetic sporopollenin from pollen grains: one-pot synthesis using ionic liquids
Authors: Chiappe C. , Rodriguez-Douton M. J. , Mozzati M. C. , Prete D. , Griesi A. , Guazzelli L. , Gemmi M. , Caporali S. , Calisi N. , Pomelli C. S. , Rossella F.
Published: July 2020

The preparation of Fe-decorated sporopollenins was achieved using pollen grains and an ionic liquid as solvent and functionalizing agent. The integrity of the organic capsules was ascertained through scanning electron microscopy studies. The presence of Fe in the capsule was investigated using FT-IR, X-ray photoemission spectroscopy and energy-dispersive X-ray spectroscopy. Electron paramagnetic resonance and magnetization measurements allowed us to demonstrate the paramagnetic behavior of our Fe-functionalized sporopollenin. A few potential applications of pollen-based systems functionalized with magnetic metal ions via ionic liquids are discussed.