bluuebunny/crossref_metadata_2025 huggingface dataset:
Decoding the transcriptomic expression and genomic methylation patterns in the tendon proper and its peritenon region in the aging horse
Authors: Pechanec Monica Y. , Mienaltowski Michael J.
Published: October 2023

Objectives

Equine tendinopathies are challenging because of the poor healing capacity of tendons commonly resulting in high re-injury rates. Within the tendon, different regions – tendon proper (TP) and peritenon (PERI) – contribute to the tendon matrix in differing capacities during injury and aging. Aged tendons have decreased repair potential; the underlying transcriptional and epigenetic changes that occur in the TP and PERI regions are not well understood. The objective of this study was to assess TP and PERI regional differences in adolescent, midlife, and geriatric horses using RNA sequencing and DNA methylation techniques.

Results

Differences existed between TP and PERI regions of equine superficial digital flexor tendons by age as evidenced by RNASeq and DNA methylation. Cluster analysis indicated that regional distinctions existed regardless of age. Genes such as DCN, COMP, FN1, and LOX maintained elevated TP expression while genes such as GSN and AHNAK were abundant in PERI. Increased gene activity was present in adolescent and geriatric populations but decreased during midlife. Regional differences in DNA methylation were also noted. Notably, when evaluating all ages of TP against PERI, five genes (HAND2, CHD9, RASL11B, ADGRD1, and COL14A1) had regions of differential methylation as well as differential gene expression.
Mitochondrial SOS: how mtDNA may act as a stress signal in Alzheimer’s disease
Authors: Gorham Isabelle K. , Barber Robert C. , Jones Harlan P. , Phillips Nicole R.
Published: October 2023

Background

Alterations in mitochondrial DNA (mtDNA) levels have been observed in Alzheimer’s disease and are an area of research that shows promise as a useful biomarker. It is well known that not only are the mitochondria a key player in producing energy for the cell, but they also are known to interact in other important intracellular processes as well as extracellular signaling and communication.

Body

This mini review explores how cells use mtDNA as a stress signal, particularly in Alzheimer’s disease. We investigate the measurement of these mtDNA alterations, the mechanisms of mtDNA release, and the immunological effects from the release of these stress signals.

Conclusion

Literature indicates a correlation between the release of mtDNA in Alzheimer’s disease and increased immune responses, showing promise as a potential biomarker. However, several questions remain unanswered and there is great potential for future studies in this area.
Evaluating the effect of Roxadustat on ventricular repolarization in patients undergoing peritoneal dialysis
Authors: Zhang Yangyang , Zhang Liang , Ge Pengcheng , Xu Ruyi , Ye Zhen
Published: October 2023

Background

Roxadustat is a novel oral medication used to treat anemia in CKD patients. Several studies have shown that Roxadustat can alleviate anemia in CKD patients by increasing hemoglobin levels and regulating iron metabolism. We aimed to evaluate the effect of Roxadustat on ventricular repolarization in PD patients. This study may provide a new integrated approach to the assessment and treatment of CKD.

Methods

The present prospective cohort study enrolled 65 CKD patients who were treated with Roxadustat and 31 CKD patients who received conventional therapy between January 2021 and June 2022. All patients were examined for ECG in the absence of clinical symptoms and compared the ECG indicators. Demographic and clinical data of all patients were collected. All data used SPSS 18.0 for statistical analyses.

Results

The T peak-to-end (Tpe) of PD patients in the Roxadustat group was remarkably slower than that of patients in the conventional group. Additionally, the Tpe/QT ratio in the conventional group was significantly elevated than that in the Roxadustat group. The results of logistic regression analysis showed that Tpe (95%CI 1.191 ~ 2.141, P = 0.002) and Roxadustat treatment (95%CI 1.357 ~ 42.121, P = 0.021) were the risk factors of PD patients with high Tp-e/QT ratio.

Conclusion

In summary, we found that Roxadustat could improve ventricular repolarization in peritoneal dialysis patients, which indicated a potential cardiovascular protective effect of Roxadustat. This study might provide a new integrated approach to the assessment and treatment of CKD.
A Lenz into the predictive power of language teacher emotion regulation and self-evaluation on L2 grit, teaching style preferences, and work engagement: a case of Chinese EFL instructors
Authors: Ma Yue
Published: October 2023

An individual’s capacity to successfully control their emotional experiences and react to them requires them to engage in a number of processes, including those that are physiological, behavioral, and cognitive. When educators engage in self-evaluation, they investigate and assess the quality of their professional work. These two teacher-related conceptions have the potential to open up valuable perspectives in the course of the professional pursuits of teachers. Even though earlier research has shown their significance, the potential implications of these factors on the resiliency and teaching style preferences of language instructors have not been emphasized. As a result, the purpose of this study was to determine the extent to which a language teacher’s ability to regulate their emotions while carrying out self-evaluation procedures may accurately predict their level of resilience as well as their preferred method of instruction. To accomplish this, 399 English as a foreign language (EFL) teachers were asked to reflect on their experiences by responding to the following related questionnaires: The Language Teacher Emotion Regulation Inventory (LTERI), The Core of Self-evaluation Questionnaire (CSEQ), the L2-teacher Grit Scale (L2TGS), Grasha Teaching Style Inventory (TSI) and the Engaged Teacher Scale (ETS). The results demonstrated that those EFL teachers who maintained healthy emotional control were grittier and more engaged. They also tended to teach in a manner focused on the students. The pedagogical implications of this research are discussed further in depth.
Anatomy of thermal unrest at a hydrothermal system: case study of the 2021–2022 crisis at Vulcano
Authors: Pailot-Bonnétat Sophie , Rafflin Victoria , Harris Andrew , Diliberto Iole Serena , Ganci Gaetana , Bilotta Guiseppe , Cappello Annalisa , Boudoire Guillaume , Grassa Fausto , Gattuso Alessandro , Ramsey Michael
Published: October 2023

Hydrothermal systems can generate phreatic and/or phreatomagmatic explosions with little warning. Understanding the temporal and spatial evolution of geophysical and geochemical signals at hydrothermal systems is crucial for detecting precursory signs to unrest and to inform on hazard. Thermal signatures of such systems are poorly defined because data records are often too short or discrete compared to activity timescales, which can be decadal. La Fossa system of Vulcano has been monitored since the 1980s and entered a period of unrest in 2021. We assessed the thermal signature of La Fossa using ground- and satellite-based data with various temporal and spatial scales. While continuously-recording stations provided continuous but point-based measurements, fumarole field vent surveys and infrared images obtained from satellite-flown sensors (ASTER and VIIRS) allowed lower temporal resolution but synoptic records to be built. By integrating this multi-resolution data set, precursory signs of unrest could retrospectively be detected from February to June 2021. Intensity of all unrest metrics increased during summer 2021, with an onset over a few days in September 2021. By September, seismic, CO2, SO2 and other geochemical metrics also indicated unrest, leading Civil Protection to raise the alert level to yellow on October 1. Heat flux, having been 4 MW in May 2019, increasing to 90 MW by September, and peaking at 120 MW in March 2022. We convolved our thermal data sets with all other monitoring data to validate a Vulcano Fossa Unrest Index (VFUI), framework of which can be potentially applied to any hydrothermal system. The VFUI highlighted four stages of unrest, none of which were clear in any single data set: background, precursory, onset and unrest. Onset was characterized by sudden release of fluids, likely caused by failure of sealed zones that had become pressurized during the precursory phase that began possibly as early as February 2021. Unrest has been ongoing for more than 18 months, and may continue for several more years. Our understanding of this system behavior has been due to hindsight, but demonstrates how multiparametric surveys can track and forecast unrest.

Graphical Abstract
Housing and management factors and breed predisposition for haemorrhagic bowel syndrome in swine
Authors: Holenweger Fabienne , Schüpbach Gertraud , Hofer Andreas , Sidler Xaver , Grahofer Alexander
Published: October 2023

Background

Haemorrhagic bowel syndrome (HBS) is a sporadically occurring disorder characterized by sudden death in pigs in combination with a pale and bloated carcass with no prior signs of disease. Most often HBS is affecting fattening pigs. Due to the good general health and performance before death as well as the time point of disease shortly prior to slaughter, this syndrome means a significant economic impact for the farm and is a major animal welfare concern. Furthermore, the cause or the causing agents have not yet been identified even though it is a worldwide known problem. The aim of this study was to detect possible risk factors for the occurrence of HBS with the focus on risk factors on herd level.

Results

Management and feeding strategies of 97 Swiss fattening herds with high and low HBS incidence were assessed and examined to identify risk factors for the disease. Having only pigs sired by the PREMO® breed in the herd showed to be a significant risk factor for HBS (Odds Ratio (OR) = 147) as compared to having other breeds or a mixture of multiple breeds. Furthermore, pigs from two or more origins per batch compared to having only one origin per batch significantly increased the disease risk (OR = 52). Farms with 1 decimetre greater feeding place width per finisher pig have a lower HBS incidence (OR = 0.07). The frequency of cleaning of the distribution pipes (split up into categories, e.g. once a month) was associated with being a HBS case farm (p < 0.05).

Conclusion

The four factors identified in this study for the occurrence of HBS represent different aspects of the environment and management. This leads to the assumption that it is a multifactorial syndrome and a thorough examination of each herd individually is necessary to mitigate disease risk. This study suggests that part of the susceptibility to HBS is genetically determined. The reduction of HBS in the herd should be the main objective to improve the economic status of the herd and improve animal welfare.
The modulation of carbohydrate intake and intermittent fasting in obese Saudi women: a pilot study
Authors: Al-jammaz Muneerah H. , Al-kalifah Abdulrahman , Al-bader Nawal Abdullah , Al-hussain Maha H.
Published: October 2023

Background

In recent years, the prevalence of overweight and obesity has increased, leading to the development of various dietary interventions as potential treatments. Two popular diets are time-restricted feeding and low-carbohydrate diets, but there is limited research on the effects of combining them. This study assessed the effects of pairing an eight-hour time-restricted feeding window with a moderate-carbohydrate diet and compared to a paired eight-hour time-restricted feeding window with a regular diet.

Results

The study involved 52 obese women divided into three groups: the first group followed an 8 h time-restricted feeding with a moderate low-carbohydrate diet (8-hTRF+mLCD), the second group followed an 8 h time-restricted feeding with a regular diet (8-hTRF), and the third group was the control group. Both 8 h TRF+mLCD and 8 h TRF groups had a decrease in TG levels compared to the control group. The HDL levels in both TRF groups were significantly higher than the control group, while LDL levels remained statistically insignificant in both TRF groups.

Conclusions

This suggests that an 8 h TRF with or without mLCD can effectively treat obesity without a change in physical activity, and combining it with a low-carbohydrate diet gives better and more rapid results.
Quasi-experimental study on fostering creativity through creative writing in German as a Foreign Language (GFL) in COVID-19
Authors: Szabó-Szettele Katinka , Kárpáti Andrea
Published: October 2023

Background: In this paper, creative language teaching and learning will be discussed in the context of subject-based development of creativity that supports learning performance. Although there are several benefits of creative teaching and learning methods, creative writing tasks are not typical in foreign language education. The main focus of this study is developing a creative writing test in GFL education. Methods: The pilot study was implemented in a primary school in Hungary in 2020/2021 and introduces a model to enhance creativity in German as a Foreign Language (GFL) classroom through creative writing tasks. In the pilot experiment, pre- and post-test design was used. Results: Results of the study show that there is significant correlation between creative writing and creative thinking skills, measured with a Language Proficiency Test for Creative Writing Skills and the Test for Creative Thinking/Drawing Development (TCT-DP) by Urban and Jellen (1996). The results indicate that our creative writing skills assessment instrument is valid for measuring creative performance in GFL, however in the development of creative writing we could not prove significant changes in the experimental group, probably because of the online teaching situation during the coronavirus disease (COVID-19) pandemic. Conclusion: Creative writing is an important tool for enhancing divergent thinking and foreign language competence. Through creative writing tasks, students engage with the language more intensively and their creative, imaginative thinking skills develop. For the evaluation of students’ creative texts analytical rubrics have been developed and they proved to be appropriate. However, despite the exciting and thought-provoking exercises, more motivation and criterion-oriented feedback is needed to support the creative learning process in an online learning environment.
Ghosting from the workplace: The impact of feedback (or lack thereof) on applicants’ psychological needs satisfaction
Authors: Wood Natasha R. , Leckfor Christina M. , Wicks Sydney G. , Hales Andrew H.
Published: October 2023

Background: Applying for a job can be a stressful experience. When a potential employer chooses to not move forward with a candidate, they can either directly tell them or cease communication altogether—known as ghosting. Ghosting has harmful consequences, such as lower basic psychological needs satisfaction than direct rejection, and there has been an apparent increase in this phenomenon by potential employers. The purpose of this study was to examine the effect of being ghosted after submitting a hypothetical job application. Methods: An international sample of unemployed people (N = 554) completed an online job application task and were randomly assigned to receive no (i.e., ghosted), impersonal, or personal feedback. Results: While our manipulation was perceived as intended, there was not an effect of condition on needs satisfaction. However, exploratory analyses suggest that ghosted participants have greater self-esteem and control than those that received personal feedback. Thus, the effect of being ghosted during a job application may not be as clear cut as previously thought. Conclusion: Although the current study did not find evidence that applicants feel worse after being ghosted than being directly rejected, such an experience can still hurt, and more research is needed to understand the long-term effects of being ghosted by a potential employer.
MOTIVATIONAL MECHANISM OF EMPLOYEE'S INITIATIVE BEHAVIOR
Authors: Esaulova Irena
Published: October 2023

In today’s business, the readiness and ability of employees to voluntarily and proactively contribute to the creation and implementation of innovations through continuous improvements in  processes, work and other aspects of  joint activities becomes an  extremely important source of  efficiency and development of the organization. This ability is manifested in initiative behavior, the management of which requires the use of methods based on understanding the nature of employees’ proactivity. The article puts forward a theoretical justification for the specific motivational mechanisms of the employee’s initiative behavior, which differ significantly from the mechanisms of normative behavior; in the development of ideas about the intra-motivational nature of proactivity, the process of launching initiative behavior is described, including the stages of creating an impulse-challenge, self-actualization and self-organization; describes the main parameters of the organizational environment that supports staff initiatives. The presented results will be useful for conducting scientific research in the field of proactive behavior, as well as for the development of HR-policy and practical measures to motivate staff.
AUTOMATION OF ALL HUMAN RESOURCE MANAGEMENT PROCESSES AS THE MAIN TREND IN 2023
Authors: Pronina V. , Fedulov D. , Tihonov A.
Published: October 2023

As a result of global changes, traditional approaches to HR management have become obsolete and there is now a trend towards full automation of HR processes. Paper-based and purely human factors in management have lost their relevance. In their place has come digitalization. Its relevance arose because of the need for flexible and rapid response to emerging issues in the course of business processes. This paper examines the major changes in human resource management using digital tools, their impact on workflow, recruitment and on onboarding, employee monitoring and maintaining the corporate climate in companies. Statistical data, charts and graphs have been used to support the theoretical aspects outlined by the authors of this paper.
A Differentiable Path-Following Method with a Compact Formulation to Compute Proper Equilibria
Authors: Cao Yiyin , Chen Yin , Dang Chuangyin
Published: October 2023

The concept of proper equilibrium was established as a strict refinement of perfect equilibrium. This establishment has significantly advanced the development of game theory and its applications. Nonetheless, it remains a challenging problem to compute such an equilibrium. This paper develops a differentiable path-following method with a compact formulation to compute a proper equilibrium. The method incorporates square-root-barrier terms into payoff functions with an extra variable and constitutes a square-root-barrier game. As a result of this barrier game, we acquire a smooth path to a proper equilibrium. To further reduce the computational burden, we present a compact formulation of an ε-proper equilibrium with a polynomial number of variables and equations. Numerical results show that the differentiable path-following method is numerically stable and efficient. Moreover, by relaxing the requirements of proper equilibrium and imposing Selten’s perfection, we come up with the notion of perfect d-proper equilibrium, which approximates a proper equilibrium and is less costly to compute. Numerical examples demonstrate that even when d is rather large, a perfect d-proper equilibrium remains to be a proper equilibrium.

History: Accepted by Antonio Frangioni, Area Editor for Design & Analysis of Algorithms-Continuous.

Funding: This work was partially supported by General Research Fund (GRF) CityU 11306821 of Hong Kong SAR Government.

Supplemental Material: The software that supports the findings of this study is available within the paper and its Supplemental Information ( https://pubsonline.informs.org/doi/suppl/10.1287/ijoc.2022.0148 ) as well as from the IJOC GitHub software repository ( https://github.com/INFORMSJoC/2022.0148 ). The complete IJOC Software and Data Repository is available at https://informsjoc.github.io/ .
Aristotle's Unified Soul: The Figure-Soul Analogy and Its Context
Authors: Hanlon Rory
Published: October 2023

abstract: I provide a novel interpretation of Aristotle's account of the unity of soul, treating it as resolving the apparent incompatibility of the existence of psychic parts and the soul's status as a unifying form. This incompatibility, I contend, rests on a problematic assumption: mereological actualism, or the claim that parts are actually distinct and prior to the whole. Aristotle successfully undermines actualism and formulates an alternative conception of parthood within De Anima 's figure-soul analogy. As triangles are only potentially present within quadrilaterals, so lower psychic parts are potential parts of higher souls. This picture treats a soul not as a mere aggregate of capacities, but as essentially unified and prior to its parts. Finally, I argue that this picture can be illuminated and must be read against the background of his hylomorphic account of the unity of form given in Metaphysics H.6.
Design and characterization of a hyperbolic-elliptical lens pair in a rapid beam steering system for single-pixel terahertz spectral imaging of the cornea
Authors: Virk Arjun S. , Harris Zachery B. , Arbab M. Hassan
Published: October 2023

Terahertz (THz) time-domain spectroscopy has been investigated for assessment of the hydration levels in the cornea, intraocular pressure, and changes in corneal topography. Previous efforts at THz imaging of the cornea have employed off-axis parabolic mirrors to achieve normal incidence along the spherical surface. However, this comes at the cost of an asymmetric field-of-view (FOV) and a long scan time because it requires raster-scanning of the collimated beam across the large mirror diameter. This paper proposes a solution by designing a pair of aspheric lenses that can provide a larger symmetric spherical FOV (9.6 mm) and reduce the scan time by two orders of magnitude using a novel beam-steering approach. A hyperbolic-elliptical lens was designed and optimized to achieve normal incidence and phase-front matching between the focused THz beam and the target curvature. The lenses were machined from a slab of high-density polyethylene and characterized in comparison to ray-tracing simulations by imaging several targets of similar sizes to the cornea. Our experimental results showed excellent agreement in the increased symmetric FOV and confirmed the reduction in scan time to about 3-4 seconds. In the future, this lens design process can be extended for imaging the sclera of the eye and other curved biological surfaces, such as the nose and fingers.
Intelligent water perimeter security event recognition based on NAM-MAE and distributed optic fiber acoustic sensing system
Authors: Sun Mingyang , Yu Miao , Wang Haoran , Song Kaiwen , Guo Xinyu , Xue Songfeng , Zhang Hongwei , Shao Yanbin , Cui Hongliang , Chang Tianying , Zhang Tianyu
Published: October 2023

Distributed optical acoustic sensing (DAS) based on phase-sensitive optical time-domain reflectometry can realize the distributed monitoring of multi-point disturbances along an optical fiber, thus making it suitable for water perimeter security applications. However, owing to the complex environment and the production of various noises by the system, continuous and effective recognition of disturbance signals becomes difficult. In this study, we propose a Noise Adaptive Mask-Masked Autoencoders (NAM-MAE) algorithm based on the novel mask mode of a Masked Autoencoders (MAE) and applies it to the intelligent event recognition in DAS. In this method, fewer but more accurate features are fed into the deep learning model for recognition by directly shielding the noise. Taking the fading noise generated by the system as an example, data on water perimeter security events collected in DAS underwater acoustic experiments are used. The NAM-MAE is compared with other models. The results indicate higher training accuracy and higher convergence speed of NAM-MAE than other models. Further, the final test accuracy reaches 96.6134%. It can be demonstrated that the proposed method has feasibility and superiority.
Out-of-plane multilayer-dielectric-grating compressor for ultrafast Ti:sapphire pulses
Authors: Werle Christian M. , Braun Cora , Eichner Timo , Hülsenbusch Thomas , Palmer Guido , Maier Andreas R.
Published: October 2023

Extreme heat loads on optics, in particular the final pulse compression gratings, are a major hurdle to overcome in the ongoing push towards high average power (kW) and high repetition rate (kHz) operation of terawatt-class Ti:sapphire lasers. Multilayer dielectric (MLD) diffraction gratings have been suggested as a potential alternative to traditionally gold-coated compressor gratings, which are plagued by high energy absorption in the top gold layer. However, to support the required bandwidth (and ultimately the desired pulse duration) with MLD gratings, the gratings have to be operated in an out-of-plane geometry near the Littrow angle. Here, we report on the design of an MLD-based out-of-plane test compressor and a matching custom stretcher. We present a full characterization of the MLD compressor, focusing on its spectral transmission and the significance of laser pulse polarization in the out-of-plane geometry. To demonstrate compression of 40 μJ pulses centered at 800 nm wavelength to 26 fs pulse duration, we use the compressor with an MLD and gold grating configuration, and fully characterize the compressed pulses. Extrapolating our results indicates that MLD-grating-based out-of-plane compressors can support near-transform-limited pulses with sub-30 fs duration and good quality, demonstrating the viability of this concept for kW-level ultrafast Ti:sapphire laser systems.
Subcarrier modulation based phase-coded coherent lidar
Authors: Song Anpeng , Jin Kai , Xu Chen , Li Jian , Guo Youming , Wei Kai
Published: October 2023

This paper presents a lean subcarrier modulation-based phase-coded coherent lidar system with a non-quadrature receiver and sampling ratio lower than the Nyquist sampling ratio. Specifically, by utilizing the drawbacks of low Doppler tolerance in encoding signals, phase information can be obtained after pulse compression, and the mirror frequency introduced by real sampling can be negligible. Validation experiments based on inverse synthetic aperture lidar are performed, with the corresponding imaging results having a resolution superior to 4 cm, demonstrating our system’s efficiency in phase acquisition that is free from frequency aliasing.
Transcriptomic comparison of two selective retinal cell ablation paradigms in zebrafish reveals shared and cell-specific regenerative responses
Authors: Emmerich Kevin , Walker Steven L. , Wang Guohua , White David T. , Ceisel Anneliese , Wang Fang , Teng Yong , Chunawala Zeeshaan , Graziano Gianna , Nimmagadda Saumya , Saxena Meera T. , Qian Jiang , Mumm Jeff S.
Published: October 2023

Retinal Müller glia (MG) can act as stem-like cells to generate new neurons in both zebrafish and mice. In zebrafish, retinal regeneration is innate and robust, resulting in the replacement of lost neurons and restoration of visual function. In mice, exogenous stimulation of MG is required to reveal a dormant and, to date, limited regenerative capacity. Zebrafish studies have been key in revealing factors that promote regenerative responses in the mammalian eye. Increased understanding of how the regenerative potential of MG is regulated in zebrafish may therefore aid efforts to promote retinal repair therapeutically. Developmental signaling pathways are known to coordinate regeneration following widespread retinal cell loss. In contrast, less is known about how regeneration is regulated in the context of retinal degenerative disease, i.e., following the loss of specific retinal cell types. To address this knowledge gap, we compared transcriptomic responses underlying regeneration following targeted loss of rod photoreceptors or bipolar cells. In total, 2,531 differentially expressed genes (DEGs) were identified, with the majority being paradigm specific, including during early MG activation phases, suggesting the nature of the injury/cell loss informs the regenerative process from initiation onward. For example, early modulation of Notch signaling was implicated in the rod but not bipolar cell ablation paradigm and components of JAK/STAT signaling were implicated in both paradigms. To examine candidate gene roles in rod cell regeneration, including several immune-related factors, CRISPR/Cas9 was used to create G0 mutant larvae (i.e., “crispants”). Rod cell regeneration was inhibited in stat3 crispants, while mutating stat5a/b, c7b and txn accelerated rod regeneration kinetics. These data support emerging evidence that discrete responses follow from selective retinal cell loss and that the immune system plays a key role in regulating “fate-biased” regenerative processes.
Unanticipated domain requirements for Drosophila Wnk kinase in vivo
Authors: Yarikipati Prathibha , Jonusaite Sima , Pleinis John M. , Dominicci Cotto Carihann , Sanchez-Hernandez David , Morrison Daryl E. , Goyal Suhani , Schellinger Jeffrey , Pénalva Clothilde , Curtiss Jennifer , Rodan Aylin R. , Jenny Andreas
Published: October 2023

WNK (With no Lysine [K]) kinases have critical roles in the maintenance of ion homeostasis and the regulation of cell volume. Their overactivation leads to pseudohypoaldosteronism type II (Gordon syndrome) characterized by hyperkalemia and high blood pressure. More recently, WNK family members have been shown to be required for the development of the nervous system in mice, zebrafish, and flies, and the cardiovascular system of mice and fish. Furthermore, human WNK2 and Drosophila Wnk modulate canonical Wnt signaling. In addition to a well-conserved kinase domain, animal WNKs have a large, poorly conserved C-terminal domain whose function has been largely mysterious. In most but not all cases, WNKs bind and activate downstream kinases OSR1/SPAK, which in turn regulate the activity of various ion transporters and channels. Here, we show that Drosophila Wnk regulates Wnt signaling and cell size during the development of the wing in a manner dependent on Fray, the fly homolog of OSR1/SPAK. We show that the only canonical RF(X)V/I motif of Wnk, thought to be essential for WNK interactions with OSR1/SPAK, is required to interact with Fray in vitro. However, this motif is unexpectedly dispensable for Fray-dependent Wnk functions in vivo during fly development and fluid secretion in the Malpighian (renal) tubules. In contrast, a structure function analysis of Wnk revealed that the less-conserved C-terminus of Wnk, that recently has been shown to promote phase transitions in cell culture, is required for viability in vivo. Our data thus provide novel insights into unexpected in vivo roles of specific WNK domains.
Working memory and attention in choice
Authors: Rustichini Aldo , Domenech Philippe , Civai Claudia , DeYoung Colin G.
Published: October 2023

We study the role of attention and working memory in choices where options are presented sequentially rather than simultaneously. We build a model where a costly attention effort is chosen, which can vary over time. Evidence is accumulated proportionally to this effort and the utility of the reward. Crucially, the evidence accumulated decays over time. Optimal attention allocation maximizes expected utility from final choice; the optimal solution takes the decay into account, so attention is preferentially devoted to later times; but convexity of the flow attention cost prevents it from being concentrated near the end. We test this model with a choice experiment where participants observe sequentially two options. In our data the option presented first is, everything else being equal, significantly less likely to be chosen. This recency effect has a natural explanation with appropriate parameter values in our model of leaky evidence accumulation, where the decline is stronger for the option observed first. Analysis of choice, response time and brain imaging data provide support for the model. Working memory plays an essential role. The recency bias is stronger for participants with weaker performance in working memory tasks. Also activity in parietal areas, coding the stored value in working, declines over time as predicted.
The menstrual cycle and the COVID-19 pandemic
Authors: Mitra Anita , Verbakel Jan Y. , Kasaven Lorraine S. , Tzafetas Menelaos , Grewal Karen , Jones Benjamin , Bennett Phillip R. , Kyrgiou Maria , Saso Srdjan
Published: October 2023

Background

The impact of COVID-19 virus on menstrual cycles in unvaccinated women is limited.

Objective

To investigate the prevalence of changes to menstrual cycle characteristics, hormonal symptoms and lifestyle changes prior to and during the COVID-19 pandemic.

Methods

A retrospective online cross-sectional survey completed by social media users between July 2020 to October 2020. Participants were living in the United Kingdom (UK), premenopausal status and, or over 18 years of age.

Main outcome(s) and measures(s)

The primary outcome was to assess changes to menstrual cycle characteristics during the pandemic following the Checklist for Reporting Results of Internet E-Surveys (CHERRIES). Secondary outcomes included assessment of hormonal and lifestyle changes.

Results

15,611 social media users completed the survey. Of which, 75% of participants experienced a change in their menstrual cycle, with significantly greater proportions reporting irregular menstrual cycles (P<0·001), bleeding duration more than seven days (P<0·001), longer mean cycle length (P<0·001) and overall bleeding duration (P<0·001). Over half the participants reported worsening of premenstrual symptoms including low mood/depression, anxiety and irritability. When stratified according to COVID-19 infection, there was no significant difference in menstrual cycle changes.

Conclusion

The COVID-19 pandemic resulted in considerable variation in menstrual cycle characteristics and hormonal symptoms. This appears to be related to societal and lifestyle changes resulting from the pandemic, rather than to the virus itself. We believe this may have an impact on the individual, as well as national economy, healthcare, and population levels, and therefore suggest this should be taken into consideration by governments, healthcare providers and employers when developing pandemic recovery plans.
Deep phenotyping of socio-emotional skills in children with typical development, neurodevelopmental disorders, and mental health conditions: Evidence from the PEERS
Authors: Anderson Vicki , Darling Simone , Hearps Stephen , Darby David , Dooley Julian , McDonald Skye , Turkstra Lyn , Brown Amy , Greenham Mardee , Crossley Louise , Charalambous George , Beauchamp Miriam H.
Published: October 2023

Objective

Socio-emotional skills, including social competence and social cognition, form the basis for robust relationships and wellbeing. Despite their importance, these skills are poorly defined and measured, particularly in children with developmental vulnerabilities. As a consequence, targets for effective management and treatment remain unclear. We aimed to i) phenotype social competence and social cognition in typically developing children (TDC) and in children with neurodevelopmental or mental health disorders (clinical groups) and ii) establish the relationships between these child-direct measures and parent ratings of social competence and behavior.

Method

Using a multi-site, cross-sectional study design, we recruited 513 TDC and 136 children with neurodevelopmental (autism spectrum disorder [ASD], attention deficit hyperactivity disorder [ADHD]) or mental health (Anxiety Disorder [ANX]) diagnoses (age range 5–15 years). We administered the Paediatric Evaluation of Emotions, Relationships and Socialisation (PEERS) to children, and parents completed standardised questionnaires rating children’s socio-emotional function.

Results

Standardised parent questionnaires revealed a global pattern of everyday socio-emotional impairment that was common to all clinical groups, while PEERS identified disorder-specific socio-cognitive profiles for children with ASD, ADHD and ANX. Compared to TDCs, children with ASD demonstrated global socio-cognitive impairment. Children with ADHD were impulsive, demonstrating difficulties managing speed accuracy trade-offs. Children with ANX exhibited slowed social decision-making, but otherwise intact skills.

Conclusions

Standardized parent questionnaires of child socio-emotional function reveal differences between children with typical and atypical development, but do not yield disorder-specific, socio-emotional profiles. In contrast, findings from the PEERS objective assessment suggest that that ASD, ADHD and ANX are associated with distinct socio-cognitive phenotypes, to more accurately guide and target management and treatment of impaired social competence.
Time course of western diet (WD) induced nonalcoholic steatohepatitis (NASH) in female and male Ldlr-/- mice
Authors: Spooner Melinda H. , Garcia-Jaramillo Manuel , Apperson K. Denise , Löhr Christiane V. , Jump Donald B.
Published: October 2023

Background

Nonalcoholic fatty liver disease (NAFLD) is a global health problem. Identification of factors contributing to the onset and progression of NAFLD have the potential to direct novel strategies to combat NAFLD.

Methods

We examined the time course of western diet (WD)-induced NAFLD and its progression to nonalcoholic steatohepatitis (NASH) in age-matched female and male Ldlr-/- mice, with time-points at 1, 4, 8, 20 and 40 weeks on the WD. Controls included Ldlr-/- mice maintained on a purified low-fat diet (LFD) for 1 and 40 weeks. The approach included quantitation of anthropometric, plasma and liver markers of disease, plus hepatic histology, lipids, oxylipins, gene expression and selected metabolites.

Results

One week of feeding the WD caused a significant reduction in hepatic essential fatty acids (EFAs: 18:2, ω6, 18:3, ω3) which preceded the decline in many C20-22 ω3 and ω6 polyunsaturated fatty acids (PUFA) and PUFA-derived oxylipins after 4 weeks on the WD. In addition, expression of hepatic inflammation markers (CD40, CD44, Mcp1, Nlrp3, TLR2, TLR4, Trem2) increased significantly in both female & male mice after one week on the WD. These markers continued to increase over the 40-week WD feeding study. WD effects on hepatic EFA and inflammation preceded all significant WD-induced changes in body weight, insulin resistance (HOMA-IR), oxidative stress status (GSH/GSSG ratio) and histological and gene expression markers of macrosteatosis, extracellular matrix remodeling and fibrosis.

Conclusions

Our findings establish that feeding Ldlr-/- mice the WD rapidly lowered hepatic EFAs and induced key inflammatory markers linked to NASH. Since EFAs have an established role in inflammation and hepatic inflammation plays a major role in NASH, we suggest that early clinical assessment of EFA status and correcting EFA deficiencies may be useful in reducing NASH severity.
Feasibility and usability of a virtual-reality-based sensorimotor activation apparatus for carpal tunnel syndrome patients
Authors: Lakshminarayanan Kishor , Shah Rakshit , Daulat Sohail R. , Moodley Viashen , Yao Yifei , Ezhil Srignana Lokesh , Ramu Vadivelan , Sengupta Puja , Madathil Deepa
Published: October 2023

Purpose

This study aimed to assess the usability of a virtual reality-assisted sensorimotor activation (VRSMA) apparatus for individual digit rehabilitation. The study had two main objectives: Firstly, to collect preliminary data on the expectations and preferences of patients with carpal tunnel syndrome (CTS) regarding virtual reality (VR) and an apparatus-assisted therapy for their affected digits. Secondly, to evaluate the usability of the VRSMA apparatus that was developed.

Methods

The VRSMA system consists of an apparatus that provides sensory and motor stimulation via a vibratory motor and pressure sensor attached to a button, and a virtual reality-based visual cue provided by texts overlaid on top of a 3D model of a hand. The study involved 10 CTS patients who completed five blocks of VRSMA with their affected hand, with each block corresponding to the five digits. The patients were asked to complete a user expectations questionnaire before experiencing the VRSMA, and a user evaluation questionnaire after completing the VRSMA. Expectations for VRSMA were obtained from the questionnaire results using a House of Quality (HoQ) analysis.

Results

In the survey for expectations, participants rated certain attributes as important for a rehabilitation device for CTS, with mean ratings above 4 for attributes such as ease of use, ease of understanding, motivation, and improvement of hand function based on clinical evidence. The level of immersion and an interesting rehabilitation regime received lower ratings, with mean ratings above 3.5. The survey evaluating VRSMA showed that the current prototype was overall satisfactory with a mean rating of 3.9 out of 5. Based on the HoQ matrix, the highest priority for development of the VRSMA was to enhance device comfort and usage time. This was followed by the need to perform more clinical studies to provide evidence of the efficacy of the VRSMA. Other technical characteristics, such as VRSMA content and device reliability, had lower priority scores.

Conclusion

The current study presents a potential for an individual digit sensorimotor rehabilitation device that is well-liked by CTS patients.
Quantitative examination of the anatomy of the juvenile sugar maple xylem
Authors: Driller Tenaya , Robinson James A. , Clearwater Mike , Holland Daniel J. , van den Berg Abby , Watson Matthew
Published: October 2023

New methodologies have enabled viable sap yields from juvenile sugar maple trees. To further improve yields, a better understanding of sap exudation is required. To achieve this, the anatomy of the xylem must first be fully characterised. We examine juvenile maple saplings using light optical microscopy (LOM) and scanning electron microscopy (SEM), looking at sections cut along differing orientations as well as macerations. From this we measure various cell parameters. We find diameter and length of vessel elements to be 28 ± 8 μm and 200 ± 50 μm, for fibre cells 8 ± 3 μm and 400 ± 100 μm, and for ray parenchyma cells 8 ± 2 μm and 50 ± 20 μm. We also examine pitting present on different cell types. On vessel elements we observe elliptical bordered pits connecting to other vessel elements (with major axis of 2.1 ± 0.7 μm and minor 1.3 ± 0.3 μm) and pits connecting to ray parenchyma (with major axis of 4 ± 2 μm and minor 2.0 ± 0.7 μm). We observe two distinct pit sizes on fibres with circular pits 0.7 ± 0.2 μm in diameter and ellipsoidal pits 1.6 ± 0.4 μm by 1.0 ± 0.3 μm. We do not observe distinct pitting patterns on different fibre types. The various cell and pit measurements obtained generally agree with the limited data available for mature trees, with the exception of vessel element and fibre length, both of which were significantly smaller than reported values.
Evaluation of a novel real-time adaptive assist-as-needed controller for robot-assisted upper extremity rehabilitation following stroke
Authors: Arantes Ana P. , Bressan Nadja , Borges Ludymila R. , McGibbon Chris A.
Published: October 2023

Rehabilitation therapy plays an essential role in assisting people with stroke regain arm function. Upper extremity robot therapy offers a number of advantages over manual therapies, but can suffer from slacking behavior, where the user lets the robot guide their movements even when they are capable of doing so by themselves, representing a major barrier to reaching the full potential of robot-assist rehabilitation. This is a pilot clinical study that investigates the use of an electromyography-based adaptive assist-as-needed controller to avoid slacking behavior during robotic rehabilitation for people with stroke. The study involved a convenience sample of five individuals with chronic stroke who underwent a robot therapy program utilizing horizontal arm tasks. The Fugl-Meyer assessment (FM) was used to document motor impairment status at baseline. Velocity, time, and position were quantified as performance parameters during the training. Arm and shoulder surface electromyography (EMG) and electroencephalography (EEG) were used to assess the controller’s performance. The cross-sectional results showed strong second-order relationships between FM score and outcome measures, where performance metrics (path length and accuracy) were sensitive to change in participants with lower functional status. In comparison, speed, EMG and EEG metrics were more sensitive to change in participants with higher functional status. EEG signal amplitude increased when the robot suggested that the robot was inducing a challenge during the training tasks. This study highlights the importance of multi-sensor integration to monitor and improve upper-extremity robotic therapy.
Rapid antiretroviral therapy initiation in patients with advanced HIV disease: 6-month outcomes of an observational cohort evaluation in Lesotho
Authors: Tiam Appolinaire , Paulin Heather , Machekano Rhoderick , Oboho Ikwo , Agyemang Elfriede , Mugyenyi Fred Asiimwe , Maama-Maime Llang , Mengistu Yohannes , Chatora Tsitsi , Mungati More , Mokone Majoalane , Mots’oane Tsietso , Masheane Annah , Tukei Vincent
Published: October 2023

For adults and adolescents, the World Health Organization defines advanced HIV disease (AHD) as a CD4 (cluster of differentiation 4) count of <200 cells/mm3 or a clinical stage 3 or 4 event. We describe clinical outcomes in a cohort of AHD patients at two regional hospitals in Lesotho. From November 2018–June 2019, we prospectively enrolled eligible patients (≥15 years) not on antiretroviral therapy (ART) presenting with WHO-defined AHD into a differentiated model of care for AHD (including rapid ART initiation) and followed them for six months. All patients received Tuberculosis (TB) symptom screening with further diagnostic testing; serum cryptococcal antigen (CrAg) screening was done for CD4 <100 cells/mm3 or WHO clinical stage 3 or 4. Medical record data were abstracted using visit checklist forms. Categorical and continuous variables were summarized using frequencies, percentages, and means, respectively. Kaplan-Meier was used to estimate survival. Of 537 HIV-positive patients screened, 150 (27.9%) had AHD of which 109 were enrolled. Mean age was 38 years and 62 (56.9%) were men. At initial clinic visit, 8 (7.3%) were already on treatment and 33% (36/109) had presumptive TB per symptom screening. Among 39/109 (40.2%) patients screened for CrAg at initial visit, five (12.8%) were CrAg-positive. Among 109 enrolled, 77 (70.6%) initiated ART at their initial clinic visit, while 32 delayed ART initiation (median delay: 14 days). Of the 109 participants enrolled, 76 (69.7%) completed the 6-month follow-up, 17 (15.6%) were lost to follow-up, 5 (4.6%) transferred to other health facilities and 11 (10.1%) died. The 6-month survival was 87.4%; among 74 patients with a viral load result, 6-month viral suppression (<1,000 copies/ml) was 85.1%. Our study found that even after the implementation of Test and Treat of ART in 2016 in Lesotho, over 25% of patients screened had AHD. Patients with AHD had a high prevalence of TB and CrAg positivity, underscoring the need to assess for AHD and rapidly initiate ART within a package of AHD care for optimal patient outcomes.