bluuebunny/crossref_metadata_2025 huggingface dataset:
Lignocellulolytic Potential of Microbial Consortia Isolated from a Local Biogas Plant: The Case of Thermostable Xylanases Secreted by Mesophilic Bacteria
Authors: Bombardi Luca , Salini Andrea , Aulitto Martina , Zuliani Luca , Andreolli Marco , Bordoli Paola , Coltro Annalaura , Vitulo Nicola , Zaccone Claudio , Lampis Silvia , Fusco Salvatore
Published: January 2024

Lignocellulose biomasses (LCB), including spent mushroom substrate (SMS), pose environmental challenges if not properly managed. At the same time, these renewable resources hold immense potential for biofuel and chemicals production. With the mushroom market growth expected to amplify SMS quantities, repurposing or disposal strategies are critical. This study explores the use of SMS for cultivating microbial communities to produce carbohydrate-active enzymes (CAZymes). Addressing a research gap in using anaerobic digesters for enriching microbiomes feeding on SMS, this study investigates microbial diversity and secreted CAZymes under varied temperatures (37 °C, 50 °C, and 70 °C) and substrates (SMS as well as pure carboxymethylcellulose, and xylan). Enriched microbiomes demonstrated temperature-dependent preferences for cellulose, hemicellulose, and lignin degradation, supported by thermal and elemental analyses. Enzyme assays confirmed lignocellulolytic enzyme secretion correlating with substrate degradation trends. Notably, thermogravimetric analysis (TGA), coupled with differential scanning calorimetry (TGA-DSC), emerged as a rapid approach for saccharification potential determination of LCB. Microbiomes isolated at mesophilic temperature secreted thermophilic hemicellulases exhibiting robust stability and superior enzymatic activity compared to commercial enzymes, aligning with biorefinery conditions. PCR-DGGE and metagenomic analyses showcased dynamic shifts in microbiome composition and functional potential based on environmental conditions, impacting CAZyme abundance and diversity. The meta-functional analysis emphasised the role of CAZymes in biomass transformation, indicating microbial strategies for lignocellulose degradation. Temperature and substrate specificity influenced the degradative potential, highlighting the complexity of environmental–microbial interactions. This study demonstrates a temperature-driven microbial selection for lignocellulose degradation, unveiling thermophilic xylanases with industrial promise. Insights gained contribute to optimizing enzyme production and formulating efficient biomass conversion strategies. Understanding microbial consortia responses to temperature and substrate variations elucidates bioconversion dynamics, emphasizing tailored strategies for harnessing their biotechnological potential.
Theoretical and Experimental Study of Different Side Chains on 3,4-Ethylenedioxythiophene and Diketopyrrolopyrrole-Derived Polymers: Towards Organic Transistors
Authors: Ren Shiwei , Zhang Wenqing , Chen Jinyang , Yassar Abderrahim
Published: January 2024

In this research, two polymers of P1 and P2 based on monomers consisting of thiophene, 3,4-Ethylenedioxythiophene (EDOT) and diketopyrrolopyrrole (DPP) are designed and obtained via Stille coupling polycondensation. The material shows excellent coplanarity and structural regularity due to the fine planarity of DPP itself and the weak non-covalent bonding interactions existing between the three units. Two different lengths of non-conjugated side chains are introduced and this has an effect on the intermolecular chain stacking, causing the film absorption to display different characteristic properties. On the other hand, the difference in the side chains does not have a significant effect on the thermal stability and the energy levels of the frontier orbitals of the materials, which is related to the fact that the materials both feature extremely high conjugation lengths and specific molecular compositions. Microscopic investigations targeting the side chains provide a contribution to the further design of organic semiconductor materials that meet device requirements. Tests based on organic transistors show a slight difference in conductivity between the two polymers, with P2 having better hole mobility than P1. This study highlights the importance of the impact of side chains on device performance, especially in the field of organic electronics.
The Role of SCAP/SREBP as Central Regulators of Lipid Metabolism in Hepatic Steatosis
Authors: Chandrasekaran Preethi , Weiskirchen Ralf
Published: January 2024

The prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) is rapidly increasing worldwide at an alarming pace, due to an increase in obesity, sedentary and unhealthy lifestyles, and unbalanced dietary habits. MASLD is a unique, multi-factorial condition with several phases of progression including steatosis, steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. Sterol element binding protein 1c (SREBP1c) is the main transcription factor involved in regulating hepatic de novo lipogenesis. This transcription factor is synthesized as an inactive precursor, and its proteolytic maturation is initiated in the membrane of the endoplasmic reticulum upon stimulation by insulin. SREBP cleavage activating protein (SCAP) is required as a chaperon protein to escort SREBP from the endoplasmic reticulum and to facilitate the proteolytic release of the N-terminal domain of SREBP into the Golgi. SCAP inhibition prevents activation of SREBP and inhibits the expression of genes involved in triglyceride and fatty acid synthesis, resulting in the inhibition of de novo lipogenesis. In line, previous studies have shown that SCAP inhibition can resolve hepatic steatosis in animal models and intensive research is going on to understand the effects of SCAP in the pathogenesis of human disease. This review focuses on the versatile roles of SCAP/SREBP regulation in de novo lipogenesis and the structure and molecular features of SCAP/SREBP in the progression of hepatic steatosis. In addition, recent studies that attempt to target the SCAP/SREBP axis as a therapeutic option to interfere with MASLD are discussed.
Structural Analyses of Designed α-Helix and β-Sheet Peptide Nanofibers Using Solid-State Nuclear Magnetic Resonance and Cryo-Electron Microscopy and Introduction of Structure-Based Metal-Responsive Properties
Authors: Nakagawa Shota , Kurokawa Minami , Kambara Ohki , Takei Toshiaki , Daidoji Kengo , Naito Akira , Takita Mao , Kawamoto Akihiro , Hirose Mika , Tamura Atsuo
Published: January 2024

The 21-residue peptide α3, which is artificially designed and consists of three repeats of 7 residues, is known to rapidly assemble into the α-helix nanofiber. However, its molecular structure within the fiber has not yet been fully elucidated. Thus, we conducted a thorough investigation of the fiber’s molecular structure using solid-state NMR and other techniques. The molecules were found to be primarily composed of the α-helix structure, with some regions near the C- and N-terminal adopting a 310-helix structure. Furthermore, it was discovered that β-sheet hydrogen bonds were formed between the molecules at both ends. These intermolecular interactions caused the molecules to assemble parallelly in the same direction, forming helical fibers. In contrast, we designed two molecules, CaRP2 and βKE, that can form β-sheet intermolecular hydrogen bonds using the entire molecule instead of just the ends. Cryo-EM and other measurements confirmed that the nanofibers formed in a cross β structure, albeit at a slow rate, with the formation times ranging from 1 to 42 days. To create peptide nanofibers that instantaneously respond to changes in the external environment, we designed several molecules (HDM1-3) based on α3 by introducing metal-binding sites. One of these molecules was found to be highly responsive to the addition of metal ions, inducing α-helix formation and simultaneously assembling into nanofibers. The nanofibers lost their structure upon removal of the metal ion. The change occurred promptly and was reversible, demonstrating that the intended level of responsiveness was attained.
Analysis of Patient Information and Differential Diagnosis with Clinical Reasoning in Pre-Clinical Medical Students
Authors: Mettarikanon Dichitchai , Tawanwongsri Weeratian
Published: January 2024

Background: The development of clinical reasoning (CR) abilities in Thai medical students during their pre-clinical years lacked well-designed establishment. Methods: This study utilized a pre-test and post-test design without a control group and was conducted at Walailak University, Thailand, in May 2022. We collected participant baseline characteristics and compared scores evaluated by the instructor and participants before and after the intervention. Additionally, we conducted a post-intervention survey on workshop satisfaction, perspectives on CR, and its learning impact. Results: Nineteen third-year medical students were included in the analysis, and twelve (63.2%) were women. The mean age was 20.6 years (standard deviation, SD: 0.5). The total score evaluated by the instructor after the intervention (8.95; SD, 1.81) was significantly higher than that before the intervention (1.68; SD, 0.67), p < 0.001. The total score evaluated by the participants after the intervention (8.22; SD = 1.44) was significantly higher than that before the intervention (2.34; SD, 2.06), p < 0.001. Overall, satisfaction was high. Conclusions: A short interactive workshop effectively taught CR principles and practice to Thai third-year medical students. These findings support the possibility of implementing this in pre-clinical curricula to prepare them for clinical practice.
A Fair Energy Allocation Algorithm for IRS-Assisted Cognitive MISO Wireless-Powered Networks
Authors: Gao Chuanzhe , Li Shidang , Wei Mingsheng , Duan Siyi , Xu Jinsong
Published: January 2024

With the rapid development of wireless communication networks and Internet of Things technology (IoT), higher requirements have been put forward for spectrum resource utilization and system performance. In order to further improve the utilization of spectrum resources and system performance, this paper proposes an intelligent reflecting surface (IRS)-assisted fair energy allocation algorithm for cognitive multiple-input single-output (MISO) wireless-powered networks. The goal of this paper is to maximize the minimum energy receiving power in the energy receiver, which is constrained by the signal-to-interference-plus-noise ratio (SINR) threshold of the information receiver in the secondary network, the maximum transmission power at the cognitive base station (CBS), and the interference power threshold of the secondary network on the main network. Due to the coupling between variables, this paper uses iterative optimization algorithms to optimize and solve different variables. That is, when solving the active beamforming variables, the passive beamforming variables are fixed; then, the obtained active beamforming variables are fixed, and the passive beamforming variables are solved. Through continuous iterative optimization, the system converges. The simulation results have verified the effectiveness of the proposed algorithm.
New Gall-Forming Insect Model, Smicronyx madaranus: Critical Stages for Gall Formation, Phylogeny, and Effectiveness of Gene Functional Analysis
Authors: Ushima Ryo , Sugimoto Ryoma , Sano Yota , Ogi Hinako , Ino Ryuichiro , Hayakawa Hiroshi , Shimada Keisuke , Tsuchida Tsutomu
Published: January 2024

The molecular mechanisms underlying insect gall formation remain unclear. A major reason for the inability to identify the responsible genes is that only a few systems can be experimentally validated in the laboratory. To overcome these problems, we established a new galling insect model, Smicronyx madaranus. Our manipulation experiments using nail polish sealing and insecticide treatment revealed an age-dependent change in gall formation by S. madaranus; adult females and larvae are responsible for gall induction and enlargement, respectively. Furthermore, it has been suggested that substances released during oviposition and larval feeding are involved in each process. Phylogenetic analysis showed that gall-forming weevils, including S. madaranus, belong to two distinct lineages that utilize different host plants. This may indicate that gall-forming traits evolved independently in these Smicronyx lineages. The efficacy of RNA interference (RNAi) in S. madaranus was confirmed by targeting the multicopper oxidase 2 gene. It is expected that the mechanisms of gall formation will be elucidated by a comprehensive functional analysis of candidate genes using RNAi and the S. madaranus galling system in the near future.
Exercise-Induced Hypoalgesia in Patients with Chronic Whiplash-Associated Disorders: Differences between Subgroups Based on the Central Sensitization Inventory
Authors: Hendriks Erwin , Coppieters Iris , Voogt Lennard , Cools Wilfried , Ickmans Kelly
Published: January 2024

Background: Physical exercise is an important element in the rehabilitation of chronic whiplash-associated disorders, with the physiological process underlying pain reduction called exercise-induced hypoalgesia. In chronic whiplash-associated disorders, exercise-induced hypoalgesia appears impaired, and the research suggests a relationship with symptoms of dysfunctional nociceptive processing, such as central sensitization. This study improves our understanding of exercise-induced hypoalgesia in chronic whiplash-associated disorders by examining the differences between the extent of exercise-induced hypoalgesia in subgroups based on scores on the central sensitization inventory (CSI). Methods: Data were collected from 135 participants with chronic whiplash-associated disorders who completed a set of questionnaires. Pain pressure thresholds and temporal summations were assessed before and after a submaximal aerobic bicycle exercise test. Results: We observed no interaction effect between exercise-induced hypoalgesia and the CSI scores for both pain pressure threshold and temporal summation. No overall statistical effect was measured in the analysis of the effect of time. The pain pressure threshold significantly related to the CSI. The temporal summation showed no correlation. Conclusions: During this study, we did not find evidence for a difference in the presence of exercise-induced hypoalgesia when the subgroups were created based on the central sensitization cluster calculator. Limited evidence was found for the influence of CSI scores on the delta pain pressure threshold.
Association of Fat Mass and Skeletal Muscle Mass with Cardiometabolic Risk Varied in Distinct PCOS Subtypes: A Propensity Score-Matched Case-Control Study
Authors: Cai Jie , Yue Jiang , Lu Nan , Li Shengxian , Zheng Jun , Huang Rong , Jiang Yihong , Shan Chang , Liu Wei , Ma Jing , Wang Lihua
Published: January 2024

(1) Background: polycystic ovarian syndrome (PCOS) is a heterogeneous syndrome with a constellation of cardiometabolic risk factors. We aimed to investigate if the association of body fat mass (BFM) and skeletal muscle mass (SMM) with cardiometabolic risk differed in PCOS subtypes. (2) Methods: 401 participants (245 PCOS and 156 controls) were assessed for anthropometric measurements, glucose–lipid profiles, reproductive hormones and body composition with propensity score-matched (PSM) analysis. The association of the cardiometabolic risk score (z score, calculated based on levels of obesity and gluco-lipid measurements) with BFM (estimated by trunk BFM/Height2) and SMM (estimated by SMM/Height2) was calculated. (3) Results: Trunk BFM/Height2 and SMM/Height2 were both positively associated with cardiometabolic risk in PCOS (trunk BFM/Height2, OR 2.33, 95% CI 1.49–3.65; SMM/Height2, OR 2.05, 95% CI 1.12–3.76). SMM/Height2 associated with increased cardiometabolic risk in obese PCOS (BMI ≥ 28 kg/m2, OR 2.27, 95% CI 1.15–4.47). For those with lower BMI (<28 kg/m2), trunk BFM/Height2 showed a higher OR in both groups (PCOS, OR 2.12, 95% CI 1.06–4.24; control 2.04, 95% CI 1.04–4.02). Moreover, distinct associations among BMI-stratified groups were validated in hierarchical clustering identifying metabolic and reproductive clusters. (4) Conclusions: BFM and SMM are synergistically associated with higher cardiometabolic risk in PCOS women. Although BFM contributes to increased cardiometabolic risk, SMM also plays a primary role in obese PCOS. Our results highlight the importance of body composition in the management of PCOS.
A Comparative Study of 1-Year Postprocedural Outcomes in Transcatheter Mitral Valve Repair in Advanced Primary Mitral Regurgitation: PASCAL vs. MitraClip
Authors: Rudolph Felix , Kirchner Johannes , Ivannikova Maria , Fortmeier Vera , Rudolph Tanja Katharina , Friedrichs Kai Peter , Rudolph Volker , Gerçek Muhammed
Published: January 2024

Both the MitraClip and PASCAL systems offer transcatheter edge-to-edge repair (TEER) solutions for mitral regurgitation. Evidence indicates a lower technical success rate for TEER in complex degenerative mitral regurgitation (DMR) cases. We conducted a retrospective analysis of patients who underwent transcatheter edge-to-edge therapy for primary mitral regurgitation with advanced anatomy, defined as mitral regurgitation effective regurgitant orifice area (MR-EROA) ≥0.40 cm2 or large flail gap (≥5 mm) or width (≥7 mm) or Barlow’s disease, that completed follow-up after 1 year. Our criteria were met by 27 patients treated with PASCAL and 18 with MitraClip. All patients exhibited a significant, equivalent short-term reduction in MR-EROA, mitral regurgitation vena contracta diameter (MR-VCD), regurgitant volume, and clinical status. At 1 year follow-up, reductions in MR-VCD, regurgitant volume, and MR-EROA remained significant for both groups without significant differences between groups. MR-Grade ≤ 1+ was achieved in 18 (66.7%) and 10 (55.6%) patients, respectively. At follow-up, no difference in hospitalization for cardiac decompensation was observed. Overall death was similar in both groups. Our study suggests that both the PASCAL and MitraClip systems significantly reduce mitral regurgitation even in advanced degenerative diseases. Within our limited data, we found no evidence of inferior performance of the PASCAL system.
Current Approach to Complications and Difficulties during Transrectal Ultrasound-Guided Prostate Biopsies
Authors: Osama Salloum , Serboiu Crenguta , Taciuc Iulian-Alexandru , Angelescu Emil , Petcu Costin , Priporeanu Tiberiu Alexandru , Marinescu Andreea , Costache Adrian
Published: January 2024

Prostate cancer is one of the most common male malignancies worldwide. It affects middle-aged men (45–60 years) and is the leading cause of cancer-related mortality in Western countries. The TRUS (trans rectal ultrasound)-guided prostate biopsy has been a standard procedure in prostate cancer detection for more than thirty years, and it is recommended in male patients with an abnormal PSA (prostate-specific antigens) or abnormalities found during digital rectal examinations. During this procedure, urologists might encounter difficulties which may cause subsequent complications. This manuscript aims to present both the complications and the technical difficulties that may occur during TRUS-guided prostate biopsy, along with resolutions and solutions found in the specialized literature. The conclusions of this manuscript will note that the TRUS-guided prostate biopsy remains a solid, cost-efficient, and safe procedure with which to diagnose prostate cancer. The complications are usually self-limiting and do not require additional medical assistance. The difficulties posed by the procedure can be safely overcome if there are no other available alternatives. Open communication with the patients improves both pre- and post-procedure compliance.
Multimorbidity of Psoriasis: A Large-Scale Population Study of Its Associated Comorbidities
Authors: Almenara-Blasco Manuel , Gracia-Cazaña Tamara , Poblador-Plou Beatriz , Laguna-Berna Clara , Carmona-Pírez Jonás , Navarro-Bielsa Alba , Prados-Torres Alexandra , Gimeno-Miguel Antonio , Gilaberte Yolanda
Published: January 2024

Introduction: Psoriasis is a chronic disease of the skin with a prevalence of 2% in the general population. The high prevalence of psoriasis has prompted the study of its comorbidities in recent decades. We designed a study to determine the prevalence of psoriasis in a large-scale, population-based cohort, to exhaustively describe its comorbidities, and to analyze which diseases are associated with psoriasis. Methods: Retrospective, observational study based on the clinical information contained in the electronic health records of the individuals in the EpiChron Cohort with a diagnosis of psoriasis (31,178 individuals) in 2019. We used logistic regression models and calculated the likelihood of the occurrence of each comorbidity based on the presence of psoriasis (p-value < 0.05). Results: The prevalence of psoriasis was 2.84%, and it was more prevalent in men (3.31% vs. 2.43%). The most frequent chronic comorbidities were disorders of lipid metabolism (35.87%), hypertension (35.50%), and other nutritional-endocrine-metabolic disorders (21.79%). The conditions most associated with psoriasis were (odds ratio; 95% confidence interval) tuberculosis (2.36; 1.24–4.49), cystic fibrosis (2.15; 1.25–3.69), amongst others. We did not find a significant association between psoriasis and hypertension or neoplasms (0.90; 0.86–0.95). Conclusions: This study revealed significant associations between psoriasis and cardiac, psychological, and musculoskeletal comorbidities.
Improved Extracorporeal Cardiopulmonary Resuscitation (ECPR) Outcomes are Associated with a Restrictive Patient Selection Algorithm
Authors: Assouline Benjamin , Mentha Nathalie , Wozniak Hannah , Donner Viviane , Looyens Carole , Suppan Laurent , Larribau Robert , Banfi Carlo , Bendjelid Karim , Giraud Raphaël
Published: January 2024

Introduction: Out-of-hospital cardiac arrest (OHCA) is a leading cause of mortality. Despite decades of intensive research and several technological advancements, survival rates remain low. The integration of extracorporeal cardiopulmonary resuscitation (ECPR) has been recognized as a promising approach in refractory OHCA. However, evidence from recent randomized controlled trials yielded contradictory results, and the criteria for selecting eligible patients are still a subject of debate. Methods: This study is a retrospective analysis of refractory OHCA patients treated with ECPR. All adult patients who received ECPR, according to the hospital algorithm, from 2013 to 2021 were included. Two different algorithms were used during this period. A “permissive” algorithm was used from 2013 to mid-2016. Subsequently, a revised algorithm, more “restrictive”, based on international guidelines, was implemented from mid-2016 to 2021. Key differences between the two algorithms included reducing the no-flow time from less than three minutes to zero minutes (implying that the cardiac arrests must occur in the presence of a witness with immediate CPR initiation), reducing low-flow duration from 100 to 60 min, and lowering the age limit from 65 to 55 years. The aim of this study is to compare these two algorithms (permissive (1) and restrictive (2)) to determine if the use of a restrictive algorithm was associated with higher survival rates. Results: A total of 48 patients were included in this study, with 23 treated under Algorithm 1 and 25 under Algorithm 2. A significant difference in survival rate was observed in favor of the restrictive algorithm (9% vs. 68%, p < 0.05). Moreover, significant differences emerged between algorithms regarding the no-flow time (0 (0–5) vs. 0 (0–0) minutes, p < 0.05). Survivors had a significantly shorter no-flow and low-flow time (0 (0–0) vs. 0 (0–3) minutes, p < 0.01 and 40 (31–53) vs. 60 (45–80) minutes, p < 0.05), respectively. Conclusion: The present study emphasizes that a stricter selection of OHCA patients improves survival rates in ECPR.
Hypertension and Dental Implants: A Systematic Review and Meta-Analysis
Authors: Hamadé Liljan , El-Disoki Salma , Chrcanovic Bruno Ramos
Published: January 2024

Purpose: The aim of the present systematic review was to investigate the influence of hypertension on the dental implant failure rate. Methods: An electronic search was undertaken in four databases, plus a manual search of journals. The I2 statistic was used to check heterogeneity and the inverse-variance method was used for the meta-analysis. The estimate of relative effect for dichotomous outcome was expressed as an odds ratio (OR). Results: The review included 24 publications. There were 4874 implants (257 failures) placed in hypertensive patients and 16,192 implants (809 failures) placed in normotensive patients. A pairwise meta-analysis showed that implants in hypertensive patients did not have a higher risk of failure than implants placed in normotensive patients (OR 1.100, p = 0.671). The log OR of implant failure between hypertensive and normotensive patients did not significantly change with the follow-up time (p = 0.824). Conclusions: This review suggests that implants in hypertensive patients do not present higher odds of failure in comparison to normotensive patients. However, further research on this topic, with the use of more rigorous criteria to diagnose patients as being hypertensive, as well as clearer information about the pharmacological management of the condition in the patients, is recommended.
Atrial Fibrillation in Patients with Hypertrophic Cardiomyopathy and Cardiac Amyloidosis: From Clinical Management to Catheter Ablation Indication
Authors: Mistrulli Raffaella , Ferrera Armando , Muthukkattil Melwyn Luis , Battistoni Allegra , Gallo Giovanna , Barbato Emanuele , Spera Francesco Raffaele , Magrì Damiano
Published: January 2024

Atrial fibrillation (AF) is the most common arrhythmia in patients affected by cardiomyopathies. Reports estimate a prevalence of 27% in patients with hypertrophic cardiomyopathy (HCM) and 40% in patients with cardiac amyloidosis (CA). The presence of AF typically results in progressive functional decline, an increased frequency of hospitalizations for heart failure, and a higher thromboembolic risk. Medical management using mainly beta-blockers or amiodarone has produced variable outcomes and a high rate of recurrence. Catheter ablation reduces symptom burden and complications despite a moderate rate of recurrence. Recent evidence suggests that an early rhythm control strategy may lead to more favorable short- and long-term outcomes. In this review, we summarize contemporary data on the management of AF in patients with cardiomyopathy (HCM and CA) with particular reference to the timing and outcomes of ablation procedures.
Does Cataract Extraction Significantly Affect Intraocular Pressure of Glaucomatous/Hypertensive Eyes? Meta-Analysis of Literature
Authors: Pasquali Andrea , Varano Luigi , Ungaro Nicola , Tagliavini Viola , Mora Paolo , Goldoni Matteo , Gandolfi Stefano
Published: January 2024

Background and Objectives: This study aimed to evaluate the effect of cataract extraction on intraocular pressure at 6, 12, and 24 months and their difference compared to the baseline in diverse glaucoma subtypes. Materials and Methods: We carried out research in the MEDLINE, Cochrane Library and EMBASE databases, as of April 2022 for relevant papers, filtered according to established inclusion and exclusion criteria. The meta-analysis evaluated the Mean Reduction and relative Standard Error in these subpopulations at predetermined times. A total of 41 groups (2302 eyes) were included in the systematic review. Due to the significant heterogeneity, they were analysed through a Random Effects Model. Results: We obtained these differences from baseline: (1) Open Angle Glaucoma at 6, 12 and 24 months, respectively: −2.44 mmHg, −2.71 mmHg and −3.13 mmHg; (2) Angle Closure Glaucoma at 6, 12 and 24 months, respectively: −6.81 mmHg, −7.03 mmHg and −6.52 mmHg; (3) Pseudoexfoliation Glaucoma at 12 months: −5.30 mmHg; (4) Ocular Hypertension at 24 months: −2.27 mmHg. Conclusions: Despite a certain variability, the reduction in ocular pressure was statistically significant at 6, 12 and 24 months in both Open Angle Glaucoma and Angle Closure Glaucoma, the latter being superior. Data for Pseudoexfoliation Glaucoma and for Ocular Hypertension are available, respectively, only at 12 months and at 24 months, both being significant.
PR Interval as a Novel Therapeutic Target of Ivabradine Therapy—Prognostic Impact of Ivabradine-Induced PR Prolongation in Heart Failure Patients
Authors: Yamamoto Riona , Kataoka Naoya , Imamura Teruhiko , Izumida Toshihide , Kinugawa Koichiro
Published: January 2024

Background: Ivabradine reduces heart rate by inhibiting the “funny current” expressed on the sinoatrial node and improves mortality and morbidity in patients with systolic heart failure and sinus tachycardia. The funny current is known to be expressed also on the atrioventricular node according to experimental studies. However, the impact of ivabradine on PR interval remained unknown. Methods: Patients with a left ventricular ejection fraction of less than 50% who received 1 month of ivabradine were screened. Electrocardiographic and echocardiographic data, particularly concerning heart rate, the PR interval, and trans-mitral flow pattern, were collected at baseline and 1-month follow-up. The primary endpoint was defined as the composite of cardiovascular death and hospital readmission for worsening heart failure following ivabradine administration. Results: In the cohort of 29 enrolled patients (median age: 66 years, 62% male), the median baseline heart rate was 86 beats per minute and the median PR interval was 168 milliseconds. Following ivabradine administration, a significant decrease of 20 beats per minute in the heart rate and a significant increase of 24 milliseconds in the PR interval were observed. The truncated interval of the A-wave, detected in the trans-mitral flow, consistently demonstrated a negative correlation with the PR interval both before and after the administration of ivabradine. During a median of 1.8 years of follow-up, six patients reached the primary endpoint. A combination of heart rate reduction and PR prolongation following ivabradine administration, both of which were independent factors associated with the primary endpoint (p < 0.05 for both), was associated with greater freedom from the primary endpoint compared with either/neither of them (p = 0.002). Conclusions: Ivabradine seems to prolong PR interval, which is a novel surrogate marker of favorable clinical outcomes in patients with systolic heart failure. This effect may be associated with the dynamics of the trans-mitral flow pattern, in conjunction with heart rate and the PR interval. Clinical implications of PR interval-guided ivabradine therapy remains the future concern.
The Relationship between Cancer Stage, Selected Immunological Parameters, Epstein–Barr Virus Infection, and Total Serum Content of Iron, Zinc, and Copper in Patients with Laryngeal Cancer
Authors: Wojnicka Julia , Grywalska Ewelina , Hymos Anna , Mertowska Paulina , Mertowski Sebastian , Charytanowicz Małgorzata , Klatka Maria , Klatka Janusz , Dolliver Wojciech Remington , Błażewicz Anna
Published: January 2024

(1) Background: the purpose of the study was to assess the relationship between cancer stage, selected immunological parameters, Epstein–Barr virus (EBV) infection, and total serum content of iron, zinc, and copper in patients with laryngeal cancer (LC). (2) Methods: serum Fe, Zn, and Cu were measured in 40 LC patients and 20 controls. Immunophenotyping of peripheral blood lymphocytes was performed by flow cytometry using fluorescent antibodies against CD3, CD4, CD8, CD19, CD25, CD69, and PD-1. Tumor and lymph node lymphocytes were analyzed by flow cytometry. EBV DNA was quantified by real-time PCR, targeting the EBNA-1 gene. Associations between serum elements, immune markers, and cancer grade/stage were evaluated using ANOVA and appropriate nonparametric tests. (3) Results: levels of Fe, Cu, and Zn were lower, while Cu/Zn was statistically higher, in patients with LC than in the control group. Correlation analysis showed a statistically significant association between the levels of these elements and parameters of the TNM (Tumor, Node, Metastasis) staging system, immunophenotype, and the amount of EBV genetic material in patients with LC who survived for more than 5 years. (4) Conclusion: the results suggest that the total serum levels of the determined micronutrients may significantly affect the immunopathogenesis and progression of LC.
Chemical and Ultrastructural Characterization of Dentin Treated with Remineralizing Dentifrices
Authors: Athanasiadou Dimitra , Eymael Denise , Hajhamid Beshr , Carneiro Karina M. M. , Prakki Anuradha
Published: January 2024

The aim of this study is to investigate dentin chemical and ultrastructural changes upon exposure to remineralizing dentifrices. Dentin disks were obtained from permanent human molars and treated for 7 days with the dentifrices: (1) C group—control (no dentifrice); (2) S group—Sensodyne Repair & Protect; (3) D group—Dentalclean Daily Regenerating Gel; and (4) DB group—D group + Dentalclean regenerating booster. Afterwards, samples were submitted to an additional 7 days of toothbrushing associated with daily acidic challenge. Samples were imaged and analyzed (days 1, 7, and 14) for Young’s modulus by atomic force microscopy (AFM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). SEM and AFM revealed precipitate deposition on dentin surfaces in groups S, D, and DB, formed as early as day 1. Surface elemental analysis showed a Si increase on all brushed surfaces. Similar surface morphology was maintained after the acidic challenge period. Bright-field TEM/SAED revealed the formation of nanocrystalline hydroxyapatite inside the dentin tubules of groups S, D, and DB after day 7. Group C presented a gradual reduction of Young’s modulus from days-1–14, whereas all remaining groups had increased values. All evaluated dentifrices led to successful formation of hydroxyapatite and increased dentin stiffness.
How Can We Best Assess Spatial Skills? Practical and Conceptual Challenges
Authors: Uttal David H. , McKee Kiley , Simms Nina , Hegarty Mary , Newcombe Nora S.
Published: January 2024

Spatial thinking skills are associated with performance, persistence, and achievement in science, technology, engineering, and mathematics (STEM) school subjects. Because STEM knowledge and skills are integral to developing a well-trained workforce within and beyond STEM, spatial skills have become a major focus of cognitive, developmental, and educational research. However, these efforts are greatly hampered by the current lack of access to reliable, valid, and well-normed spatial tests. Although there are hundreds of spatial tests, they are often hard to access and use, and information about their psychometric properties is frequently lacking. Additional problems include (1) substantial disagreement about what different spatial tests measure—even two tests with similar names may measure very different constructs; (2) the inability to measure some STEM-relevant spatial skills by any existing tests; and (3) many tests only being available for specific age groups. The first part of this report delineates these problems, as documented in a series of structured and open-ended interviews and surveys with colleagues. The second part outlines a roadmap for addressing the problems. We present possibilities for developing shared testing systems that would allow researchers to test many participants through the internet. We discuss technological innovations, such as virtual reality, which could facilitate the testing of navigation and other spatial skills. Developing a bank of testing resources will empower researchers and educators to explore and support spatial thinking in their disciplines, as well as drive the development of a comprehensive and coherent theoretical understanding of spatial thinking.
Advancements in Metal Additive Manufacturing: A Comprehensive Review of Material Extrusion with Highly Filled Polymers
Authors: Sadaf Mahrukh , Bragaglia Mario , Slemenik Perše Lidija , Nanni Francesca
Published: January 2024

Additive manufacturing (AM) has attracted huge attention for manufacturing metals, ceramics, highly filled composites, or virgin polymers. Of all the AM methods, material extrusion (MEX) stands out as one of the most widely employed AM methods on a global scale, specifically when dealing with thermoplastic polymers and composites, as this technique requires a very low initial investment and usage simplicity. This review extensively addresses the latest advancements in the field of MEX of feedstock made of polymers highly filled with metal particles. After developing a 3D model, the polymeric binder is removed from the 3D-printed component in a process called debinding. Furthermore, sintering is conducted at a temperature below the melting temperature of the metallic powder to obtain the fully densified solid component. The stages of MEX-based processing, which comprise the choice of powder, development of binder system, compounding, 3D printing, and post-treatment, i.e., debinding and sintering, are discussed. It is shown that both 3D printing and post-processing parameters are interconnected and interdependent factors, concurring in determining the resulting mechanical properties of the sintered metal. In particular, the polymeric binder, along with its removal, results to be one of the most critical factors in the success of the entire process. The mechanical properties of sintered components produced through MEX are generally inferior, compared with traditional techniques, as final MEX products are more porous.
Benthic Colonization on New Materials for Marine Ecosystem Restoration in Porto Cesareo, Italy
Authors: Bracho-Villavicencio Carolina , Matthews-Cascon Helena , García-Durán Marc , Vélez Xavier , Lago Nicola , Busquier Laura , Rossi Sergio
Published: January 2024

Suitable colonization materials are a pursued target in marine restoration programs. Known for making nutrients available while reducing pollutants and the risk of pathogens in terrestrial ecosystems, Biochar and Bioferment materials of organic origin were tested during a two-year experiment. We tested the efficacy of these materials for restoration purposes through experimental concrete tiles treated with Biochar (B) and Bioferment (F) and tiles made of concrete, which were used as controls (Ct) for the colonization of marine organisms in the marine protected area of Porto Cesareo, Southern Italy (20 m depth). Tiles were monitored for photographs from October 2019 to September 2021. Initially, Biochar treatment presented a higher percentage of total benthic cover (81.23 ± 2.76, median ± SE), differing from Bioferment treatment and control tiles (45.65 ± 5.43 and 47.95 ± 3.69, respectively). Significant interaction between treatments and times suggests changes in community structure related to Polychaeta cover increase in Bioferment and control materials from the second monitoring time. Furthermore, the underwater instability of Bioferment on the tiles could explain the similarity with control tiles in marine organisms’ covers. Hence, Biochar is shown to be a material with optimal stability in seawater, demonstrating greater capacity for marine organisms’ colonization in less time compared to the other two materials.
The Construction Conditions of a Pre-Piling Template for Foundations of Offshore Structures
Authors: Won Deokhee , Seo Jihye , Kwon Osoon , Park Hae-Young , Kang Hyoun
Published: January 2024

The foundations of offshore wind power can be classified as floating, tripod, jacket, monopile, or gravity-based, depending on the support type. In the case of tripod- and jacket-type supports, the structures require precise construction. There are two main methods for installing substructures: post- and pre-piling. The post-piling method involves moving the completed substructure to the site and fixing it to the seabed by inserting a pile into the leg pile and driving it, allowing it to be constructed without special off-shore equipment; however, the construction period is long. Contrarily, the precision of foundation installation can be improved by installing a pre-piling template, which is special equipment that serves as a basic structure, on the seabed in advance, and subsequently inserting substructures. This study presents a new type of underwater pre-piling template and method for achieving optimal construction environment conditions. Construction precision was analyzed based on the wave condition, current speed, winch speed, wave direction, and current direction while the under-water template was anchored to the seabed. It was found that the wave conditions, winch speed, and vessel type had a significant influence. The results obtained considering the Douglas sea scale show that precise construction could only be achieved within Grade 2 for general barge ships, while jack-up barge ships could be used even at Grade 3 or higher. The higher the winch speed, the more stable construction becomes possible, and jack-up barges show greater constructability than general barges.
Fungal Abundance and Diversity in the Mariana Trench, the Deepest Ecosystem on Earth
Authors: Varrella Stefano , Barone Giulio , Corinaldesi Cinzia , Giorgetti Alessio , Nomaki Hidetaka , Nunoura Takuro , Rastelli Eugenio , Tangherlini Michael , Danovaro Roberto , Dell’Anno Antonio
Published: January 2024

Hadal trenches host abundant and diversified benthic prokaryotic assemblages, but information on benthic fungi is still extremely limited. We investigated the fungal abundance and diversity in the Challenger Deep (at ca. 11,000 m depth) and the slope of the Mariana Trench in comparison with three sites of the adjacent abyssal plain. Our results indicate that trench sediments are a hotspot of fungal abundance in terms of the 18S rRNA gene copy number. The fungal diversity (as the number of amplicon sequence variants, ASVs) was relatively low at all sites (10–31 ASVs) but showed a high turnover diversity among stations due to the presence of exclusive fungal taxa belonging to Aspergillaceae, Trichosphaeriaceae, and Nectriaceae. Fungal abundance and diversity were closely linked to sediment organic matter content and composition (i.e., phytopigments and carbohydrates), suggesting a specialization of different fungal taxa for the exploitation of available resources. Overall, these findings provide new insights into the diversity of deep-sea fungi and the potential ecological role in trench sediments and pave the way for a better understanding of their relevance in one of the most extreme ecosystems on Earth.
Looking at the Data on Smoking and Post-COVID-19 Syndrome—A Literature Review
Authors: Trofor Antigona , Robu Popa Daniela , Melinte Oana , Trofor Letiția , Vicol Cristina , Grosu-Creangă Ionela , Crișan Dabija Radu , Cernomaz Andrei
Published: January 2024

Long COVID is a recently described entity that is responsible for significant morbidity and that has consequences ranging from mild to life-threatening. The underlying mechanisms are not completely understood, and treatment options are currently limited, as existing data focus more on risk factors and predictors. Smoking has been reported as a risk factor for poor outcomes of acute SARS-CoV-2 infection and seems to also play a role in mediating post-COVID-19 symptoms. We aimed to review relevant work addressing the interaction between smoking and long COVID in order to characterize smoking’s role as a risk factor and possibly identify new research directions. Methods: The PubMed/MEDLINE database was searched using the keywords ‘smoking’, ‘long COVID’, and ‘post-acute COVID’ to identify relevant English-language articles published up to October 2023. Results and conclusions: From the 374 initial hits, a total of 36 papers were deemed relevant to the aim of the review. There was significant variability concerning the ways in which tobacco usage was quantified and reported; still, there is compelling evidence linking smoking to an increased risk of developing manifestations of post-acute-COVID disease. Some clinical conditions, such as dyspnea, cardiovascular symptoms, and cognitive or mental-health impairment, seem to be relatively strongly associated with smoking, while the connection between smoking and upper-airway involvement seems less certain. The available data support recommending smoking cessation as a clinical tool for the prevention of long COVID.
The Relationship between Fine Particle Matter (PM2.5) Exposure and Upper Respiratory Tract Diseases
Authors: Zaręba Łukasz , Piszczatowska Katarzyna , Dżaman Karolina , Soroczynska Karolina , Motamedi Parham , Szczepański Mirosław , Ludwig Nils
Published: January 2024

PM2.5 is one of the most harmful components of airborne pollution and includes particles with diameters of less than 2.5 μm. Almost 90% of the world’s population lives in areas with poor air quality exceeding the norms established by the WHO. PM2.5 exposure affects various organs and systems of the human body including the upper respiratory tract which is one of the most prone to its adverse effects. PM2.5 can disrupt nasal epithelial cell metabolism, decrease the integrity of the epithelial barrier, affect mucociliary clearance, and alter the inflammatory process in the nasal mucosa. Those effects may increase the chance of developing upper respiratory tract diseases in areas with high PM2.5 pollution. PM2.5’s contribution to allergic rhinitis (AR) and rhinosinusitis was recently thoroughly investigated. Numerous studies demonstrated various mechanisms that occur when subjects with AR or rhinosinusitis are exposed to PM2.5. Various immunological changes and alterations in the nasal and sinonasal epithelia were reported. These changes may contribute to the observations that exposure to higher PM2.5 concentrations may increase AR and rhinosinusitis symptoms in patients and the number of clinical visits. Thus, studying novel strategies against PM2.5 has recently become the focus of researchers’ attention. In this review, we summarize the current knowledge on the effects of PM2.5 on healthy upper respiratory tract mucosa and PM2.5’s contribution to AR and rhinosinusitis. Finally, we summarize the current advances in developing strategies against PM2.5 particles’ effects on the upper respiratory tract.
Portugal’s Crowdfunding: A Systematic Literature Review
Authors: Torres Bruno , Serrasqueiro Zélia , Oliveira Márcio
Published: January 2024

This study aims to analyze and classify the evolution of crowdfunding in Portugal from 2014 to 2020, addressing the central question, “What is the evolution of literature on crowdfunding and its research focuses in Portugal?”. Additionally, it investigates, through the sub-question, if crowdfunding is perceived as an alternative form of financing. The methodology employs a systematic review, covering four thematic areas: (1) research focus—concepts; (2) research method—quantitative/qualitative identification; (3) geographical area—countries of study; (4) innovation—future research areas. The research begins with Google Scholar, followed by a more specific search of the B-On database, focusing on the Portuguese context. Results highlight the scarcity of research in Portugal, emphasizing the nascency of crowdfunding in the country. The study reveals the importance of investor behavior, influenced by platform security and regulations. Growth in crowdfunding in Portugal is anticipated, attracting multidisciplinary interest but emphasizing the need for more comprehensive studies. Despite limitations in data availability, the study provides valuable insights for entrepreneurs seeking alternative financing in Portugal, demonstrating crowdfunding as an alternative financing method. Integration of crowdfunding with technology, especially blockchain, is suggested as a potentially disruptive system, paving the way for future research and innovations.