bluuebunny/crossref_metadata_2025 huggingface dataset:
Hair Growth-Promoting Effect of Hydrangea serrata (Thunb.) Ser. Extract and Its Active Component Hydrangenol: In Vitro and In Vivo Study
Authors: Park Soyoon , Kim Hyunjae , Ahn Hye Shin , Na Changseon , Shin Yu-Kyong
Published: September 2024

With the escalating prevalence of hair loss, the demand for effective hair loss treatment has surged. This study evaluated the effects of hot water extract of Hydrangea serrata (Thunb.) Ser. leaf (WHS) on hair growth, employing cell cultures, mice, and human skin organoid models. Both WHS and hydrangenol were found to enhance 5α-reductase inhibitory activity. WHS and hydrangenol have been shown to stimulate dermal papilla cell (DPC) growth, potentially through factors like keratinocyte growth factor (KGF), fibroblast growth factor 10 (FGF10), and transforming growth factor-β1 (TGF-β1). They also elevated the expression levels of keratin genes (K31 and K85) and the ceramide synthase (CerS3) gene, crucial clinical indicators of hair health. Furthermore, they exhibited notable anti-inflammatory and anti-androgenic properties by reducing the levels of tumor necrosis factor-α (TNF-α) and androgen signaling molecules, including androgen receptor (AR) and dickkopf-1 (DKK-1) gene expression. Oral administration of WHS to C57BL/6 mice for 3 weeks confirmed its hair growth-promoting effects, improving hair growth parameters and gene expression without significant changes in hair weight. Additionally, in a human skin organoid model, WHS was found to stimulate hair formation and augment the expression of follicle markers. These findings position WHS as a promising nutraceutical for promoting hair health, as evidenced by its efficacy in both in vitro and in vivo models.
Integration Linkage Mapping and Comparative Transcriptome Analysis to Dissect the Genetic Basis of Rice Salt Tolerance Associated with the Germination Stage
Authors: Geng Leiyue , Zou Tuo , Zhang Wei , Wang Shuo , Yao Yutao , Zheng Zhenyu , Du Qi , Han Longzhi
Published: September 2024

Soil salinity poses a serious threat to rice production. The salt tolerance of rice at the germination stage is one of the major determinants of stable stand establishment, which is very important for direct seeding in saline soil. The complexity and polygenic nature of salt tolerance have limited the efficiency of discovering and cloning key genes in rice. In this study, an RIL population with an ultra-high-density genetic map was employed to investigate the salt-tolerant genetic basis in rice, and a total of 20 QTLs were detected, including a major and stable QTL (qRCL3-1). Subsequently, salt-specific DEGs from a comparative transcriptome analysis were overlaid onto annotated genes located on a stable QTL interval, and eight putative candidate genes were further identified. Finally, from the sequence alignment and variant analysis, OsCam1-1 was confirmed to be the most promising candidate gene for regulating salinity tolerance in rice. This study provides important information for elucidating the genetic and molecular basis of rice salt tolerance at the germination stage, and the genes detected here will be useful for improvements in rice salt tolerance.
Blockade of mTORC1 via Rapamycin Suppresses 27-Hydroxycholestrol-Induced Inflammatory Responses
Authors: Kang Nakyung , Kim Jaesung , Kwon Munju , Son Yonghae , Eo Seong-Kug , Baryawno Ninib , Kim Byoung Soo , Yoon Sik , Oh Sae-Ock , Lee Dongjun , Kim Koanhoi
Published: September 2024

Atherosclerosis is characterized by the deposition and accumulation of extracellular cholesterol and inflammatory cells in the arterial blood vessel walls, and 27-hydroxycholesterol (27OHChol) is the most abundant cholesterol metabolite. 27OHChol is an oxysterol that induces immune responses, including immune cell activation and chemokine secretion, although the underlying mechanisms are not fully understood. In this study, we investigated the roles of the mechanistic target of rapamycin (mTOR) in 27HChol-induced inflammation using rapamycin. Treating monocytic cells with rapamycin effectively reduced the expression of CCL2 and CD14, which was involved with the increased immune response by 27OHChol. Rapamycin also suppressed the phosphorylation of S6 and 4EBP1, which are downstream of mTORC1. Additionally, it also alleviates the increase in differentiation markers into macrophage. These results suggest that 27OHChol induces inflammation by activating the mTORC1 signaling pathway, and rapamycin may be useful for the treatment of atherosclerosis-related inflammation involving 27OHchol.
Physiologically Achievable Concentration of 2-Deoxy-D-Glucose Stimulates IFN-γ Secretion in Activated T Cells In Vitro
Authors: Repas Jernej , Frlic Tjaša , Snedec Tadeja , Kopitar Andreja Nataša , Sourij Harald , Janež Andrej , Pavlin Mojca
Published: September 2024

2-deoxy-D-glucose (2DG) is a glycolysis and protein N-glycosylation inhibitor with promising anti-tumor and immunomodulatory effects. However, 2DG can also suppress T cell function, including IFN-γ secretion. Few human T cell studies have studied low-dose 2DG, which can increase IFN-γ in a Jurkat clone. We therefore investigated 2DG’s effect on IFN-γ in activated human T cells from PBMCs, with 2DG treatment commenced either concurrently with activation or 48 h after activation. Concurrent 2DG treatment decreased IFN-γ secretion in a dose-dependent manner. However, 2DG treatment of pre-activated T cells had a hormetic effect on IFN-γ, with 0.15–0.6 mM 2DG (achievable in vivo) increasing and >2.4 mM 2DG reducing its secretion. In contrast, IL-2 levels declined monotonously with increasing 2DG concentration. Lower 2DG concentrations reduced PD-1 and increased CD69 expression regardless of treatment timing. The absence of increased T-bet or Eomes expression or IFNG transcription suggests another downstream mechanism. 2DG dose-dependently induced the unfolded protein response, suggesting a possible role in increased IFN-γ secretion, possibly by increasing the ER folding capacity for IFN-γ via increased chaperone expression. Overall, low-dose, short-term 2DG exposure could potentially improve the T cell anti-tumor response.
A Training-Free Latent Diffusion Style Transfer Method
Authors: Xiang Zhengtao , Wan Xing , Xu Libo , Yu Xin , Mao Yuhan
Published: September 2024

Diffusion models have attracted considerable scholarly interest for their outstanding performance in generative tasks. However, current style transfer techniques based on diffusion models still rely on fine-tuning during the inference phase to optimize the generated results. This approach is not merely laborious and resource-demanding but also fails to fully harness the creative potential of expansive diffusion models. To overcome this limitation, this paper introduces an innovative solution that utilizes a pretrained diffusion model, thereby obviating the necessity for additional training steps. The scheme proposes a Feature Normalization Mapping Module with Cross-Attention Mechanism (INN-FMM) based on the dual-path diffusion model. This module employs soft attention to extract style features and integrate them with content features. Additionally, a parameter-free Similarity Attention Mechanism (SimAM) is employed within the image feature space to facilitate the transfer of style image textures and colors, while simultaneously minimizing the loss of structural content information. The fusion of these dual attention mechanisms enables us to achieve style transfer in texture and color without sacrificing content integrity. The experimental results indicate that our approach exceeds existing methods in several evaluation metrics.
BACH: A Tool for Analyzing Blockchain Transactions Using Address Clustering Heuristics
Authors: Caringella Michele , Violante Francesco , De Lucci Francesco , Galantucci Stefano , Costantini Matteo
Published: September 2024

Cryptocurrencies have now become an emerging blockchain-based payment technology; among them, bitcoin is the best known and most widely used. Users on these networks are pseudo-anonymous, meaning that while all transactions from an address are transparent and searchable by anyone, the users’ true identities are not directly revealed; to preserve their privacy, users often use many different addresses. In recent years, some studies have been conducted regarding analyzing clusters of bitcoin addresses that, according to certain heuristics, belong to the same entity. This capability provides law enforcement with valuable information for investigating illegal activities involving cryptocurrencies. Clustering methods that rely on a single heuristic often fail to accurately and comprehensively cluster multiple addresses. This paper proposes Bitcoin Address Clustering based on multiple Heuristics (BACH): a tool that uses three different clustering heuristics to identify clusters of bitcoin addresses, which are displayed through a three-dimensional graph. The results lead to several analyses, including a comparative evaluation of WalletExplorer, which is a similar address clustering tool. BACH introduces the innovative feature of visualizing the internal structure of clusters in a graphical format. The study also shows how the combined use of different heuristics provides better results and more complete clusters than those obtained from their individual use.
Mitogenome-Based Phylogeny with Divergence Time Estimates Revealed the Presence of Cryptic Species within Heptageniidae (Insecta, Ephemeroptera)
Authors: Guo Zhi-Qiang , Shen Chen-Yang , Cheng Hong-Yi , Chen Yu-Xin , Wu Hui-Yuan , Storey Kenneth B. , Yu Dan-Na , Zhang Jia-Yong
Published: September 2024

Heptageniidae are known for their flat heads and bodies and are divided into three subfamilies. Despite the extensive diversity within this group and considerable efforts made to understand their evolutionary history, the internal classifications and origin time of Heptageniidae remains controversial. In this study, we newly sequenced 17 complete mitogenomes of Heptageniidae to reconstruct their phylogenetic positions within this family. Because of the ambiguous time of origin, our study also estimated the divergence time within Heptageniidae based on five fossil calibration points. The results of BI and ML trees all highly supported the monophyly of Heptageniidae and three subfamilies. The phylogenetic relationship of Rhithrogeninae + (Ecdyonurinae + Heptageniinae) was also recovered. The divergence time showed that Heptageniidae originated from 164.38 Mya (95% HPD, 150.23–181.53 Mya) in the mid-Jurassic, and Rhithrogeninae originated from 95.54 Mya (95% HPD, 73.86–120.19 Mya) in the mid-Cretaceous. Ecdyonurinae and Heptageniinae began to diverge at 90.08 Mya (95% HPD, 68.81–113.16 Mya) in the middle Cretaceous. After morphological identification, analysis of the mitogenome’s composition, genetic distance calculation, phylogenetic analysis, and divergence time calculation, we suggest that two different populations of Epeorus montanus collected from Aksu, Xinjiang Uygur Autonomous Region (40°16′ N, 80°26′ E) and Xinyuan, Xinjiang Uygur Autonomous Region (43°20′ N, 83°43′ E) in China are cryptic species of E. montanus, but further detailed information on their morphological characteristics is needed to fully identify them.
Evidence of Cuticle Chemicals of Heortia vitessoides (Lepidoptera: Crambidae) Larvae Influencing the Aggregation Behavior of Conspecific Larvae
Authors: Yang Xinya , Li Guangsheng , Wang Cai
Published: September 2024

Heortia vitessoides (Lepidoptera: Crambidae) is a severe pest of Aquilaria plants, which produce high-priced agarwood. The larval stage of this pest is gregarious, usually forming large aggregates during young instars and becoming solitary during the fifth instar. We hypothesize that the cuticle chemicals of young-instar H. vitessoides larvae could promote larval aggregating, whereas the cuticle chemicals of late-instar larvae would no longer attract young-instar larvae. In this study, two-choice tests were conducted to evaluate the effect of cuticle extracts of second- and fifth-instar H. vitessoides larvae on the aggregation preference of second-instar larvae. Results show that significantly more larvae aggregated on the leaves treated with the hexane extract of second-instar H. vitessoides larvae than on untreated leaves. However, the hexane extract of fifth-instar larvae had no significant effect on the aggregation preference of the second-instar conspecific larvae. Interestingly, acetone extracts of both second- and fifth-instar H. vitessoides larvae repelled the second-instar conspecific larvae throughout the 8 h experiment. Our study shows that cuticle chemicals of H. vitessoides larvae may play a role in the group dynamics of this pest, which may contribute to screening novel attractants and repellents for H. vitessoides. Detailed chemical analyses of the extracts and identification of the compounds involved in larval attracting and repelling would be valuable in future studies.
Embolization of a Large Right-Coronary-Artery-to-Left-Atrium Fistula in a Three-Year-Old Child: A Case Report
Authors: Krasic Stasa , Butera Gianfranco , Topic Vesna , Vukomanovic Vladislav
Published: September 2024

Objectives: Coronary artery fistulas (CAFs) are rare congenital anomalies with an occurrence rate of 0.002–0.3%. The right coronary artery (RCA) is reportedly the most common site of origin of CAFs, but fistulas draining to the left atrium (LA) are rare. We presented a three-year-old boy with a symptomatic congenital RCA-to-LA fistula, which was successfully percutaneously occluded with an Amplatzer vascular plug 4 (AVP4). Case report: The diagnosis was made by echocardiography when he was two months old. During the follow-up period of 2 years, a progressive dilatation of the RCA and enlargement of the left ventricle was detected, so treatment for congestive heart failure was initiated. At the age of three, the patient presented with a history of occasional mild central chest pain and discomfort and mild dyspnea on exertion. On a 24 h ECG Holter monitor, the depression of ST segments was registered. CT angiography highlighted a large type B RCA fistula to the LA, which extended along the atrioventricular sulcus. The proximal RCA diameter was 7 mm. The fistula was tortuous, with segmental narrowing and three curves. Cardiac catheterization was performed across the right femoral artery on the three-year-old boy (body weight: 13 kg). Across the 4F Judkins right guiding catheter, an AVP4 of 5 mm was placed in the distal part of the CAF connected with the delivery cable. After 15 min, ECG changes were not registered, so the device was released. Immediate post-deployment angiography demonstrated complete CAF occlusion, with satisfying flow in the distal coronary artery. The patient was discharged after four days. In the short-term follow-up period, the boy was symptom-free. Conclusions: In our experience, given the existence of the left-to-left shunt and the more pronounced exercise-induced coronary steal phenomenon that occurs in medium-sized and large CAFs, occlusion is necessary to prevent the further progression of clinical signs and symptoms.
Can a Continuous Wound Infiltration System Replace Intravenous Patient-Controlled Analgesia for Postoperative Pain Management after a Single-Port Access Laparoscopy?
Authors: Kang Jun-Hyeok , Seo Yumi , Lee Hyunji , Kim Woo Young , Paik E Sun
Published: September 2024

Background: The aim of this study was to determine whether continuous wound infiltration (CWI) can replace intravenous patient-controlled analgesia (IV PCA) and to investigate effective pain control strategies after a single-port access (SPA) laparoscopy for adnexal disease. Methods: A total of 470 patients (the CWI group [n = 109], the IV PCA group [n = 198], and the combined group [n = 163]) who underwent an SPA adnexal laparoscopy and who received CWI or IV PCA for postoperative pain management were retrospectively reviewed. The numeric rating scale (NRS) pain score at 6, 12, 24, and 48 h (h) after surgery and the total amount of fentanyl administered via IV PCA were collected. The incidence of postoperative nausea and vomiting (PONV) and the total amount of rescue antiemetic drugs administered were also evaluated. Results: The mean NRS pain scores at 6 h (combined vs. PCA vs. CWI, 3.08 vs. 3.44 vs. 3.96, p < 0.001), 12 h (2.10 vs. 2.65 vs. 2.82, p < 0.001), and 24 h (1.71 vs. 2.01 vs. 2.12, p < 0.001) after surgery were significantly lower in the combined group. CWI showed a similar pain-reduction effect after surgery compared to IV PCA, except for the acute phase (within 6 h after surgery). The incidence of PONV during the entire hospitalization period was significantly lower in the CWI group compared to the groups using IV PCA (p < 0.05). The combined group had a significantly lower incidence of PONV and use of rescue antiemetics than the IV PCA group (p < 0.05). The combined group required significantly less total PCA fentanyl compared to the IV PCA group (combined vs. PCA, 622.1 μg vs. 703.1 μg, p < 0.001). Conclusions: CWI is an effective alternative to IV PCA and has fewer side effects. Combined use of CWI and IV PCA may be an ideal pain management strategy, offering a strong pain-reduction effect and only moderate side effects.
Prospective Study of Preoperative Negative Affect and Postoperative Pain in Patients Undergoing Thoracic Surgery: The Moderating Role of Sex
Authors: Franqueiro Angelina R. , Wilson Jenna M. , He Jingui , Azizoddin Desiree R. , Karamnov Sergey , Rathmell James P. , Soens Mieke , Schreiber Kristin L.
Published: September 2024

Objective: Preoperative negative affect is a risk factor for worse postoperative pain, but research investigating this association among patients undergoing thoracic surgery is inconsistent. Additionally, female patients often report greater negative affect and postoperative pain than males. This prospective observational study investigated the association between preoperative negative affect and postoperative pain after thoracic surgery and whether this association differed by sex. Methods: Patients (n = 105) undergoing thoracic surgery completed preoperative assessments of pain and negative affect (PROMIS anxiety and depression short forms). Patients reported their daily worst pain over the first 7 postoperative days, and an index score of acute postoperative pain was created. Six months after surgery, a subsample of patients (n = 60) reported their worst pain. Results: Higher levels of preoperative anxiety (r = 0.25, p = 0.011) and depression (r = 0.20, p = 0.042) were associated with greater acute postoperative pain, but preoperative negative affect was not related to chronic postsurgical pain (anxiety: r = 0.19, p = 0.16; depression: r = −0.01, p = 0.94). Moderation analyses revealed that the associations between both preoperative anxiety (b = 0.12, 95% CI [0.04, 0.21], p = 0.004) and depression (b = 0.15, 95% CI [0.04, 0.26], p = 0.008) with acute postoperative pain were stronger among females than males. Similarly, the association between preoperative anxiety and chronic postsurgical pain was stronger among females (b = 0.11, 95% CI [0.02, 0.20], p = 0.022), but the association between preoperative depression and chronic pain did not differ based on sex (b = 0.13, 95% CI [−0.07, 0.34], p = 0.201]). Conclusions: Our findings suggest that negative affect may be especially important to the experience of pain following thoracic surgery among female patients, whose degree of preoperative anxiety may indicate vulnerability to progress to a chronic pain state. Preoperative interventions aimed at reducing negative affect and pain may be particularly useful among females with high negative affect before thoracic surgery.
Frequency and Determinants of Levothyroxine Therapy Initiation for Veterans with Subclinical Hypothyroidism
Authors: Terlea Andreea , Toloza Freddy J. K. , Owen Richard R. , Williams James S. , Knox Micheal , Dishongh Katherine , Thostenson Jeff D. , Singh Ospina Naykky M. , Brito Juan P. , Maraka Spyridoula
Published: September 2024

Background/Objectives: There is evidence of overtreatment in patients with subclinical hypothyroidism (SCH). We aimed to identify the proportion of patients treated for SCH and the determinants of thyroid hormone therapy initiation. Methods: We included a random sample of adult Veterans diagnosed with SCH from 1 January 2016 to 31 December 2018 and conducted univariate and multivariable logistic regression to identify factors associated with levothyroxine initiation. Results: Out of 229 Veterans with SCH [90.0% male, 87.2% White, 99.1% non-Hispanic, median age (interquartile range; IQR) 68 (17) years], 27.5% were treated with levothyroxine. The treated group had a higher proportion of White patients (95.2% vs. 84.2%, p = 0.039), a higher thyrotropin level [median (IQR), 6.98 (2.06) mIU/L vs. 6.14 (1.10) mIU/L, p = 0.0002], a higher proportion of patients with thyrotropin level ≥ 10 mIU/L (11.1% vs. 3.0%, p = 0.021), a lower frequency of confirmatory thyroid testing before initiating levothyroxine (49.2% vs. 97.0%, p < 0.0001), and a similar frequency of thyroid autoimmunity testing (3.2% vs. 0.6%, p = 0.18) compared to the untreated group. In a multivariable logistic regression analysis, White race (OR = 4.50, 95% CI 1.19 to 17.08, p = 0.026) and index thyrotropin level [OR = 1.71, 95% CI 1.24 to 2.35, p = 0.001; for every SD increase (1.6 mIU/L)] were associated with higher odds of treatment. Conclusions: Three in 10 Veterans with SCH received levothyroxine, often based on a single abnormal thyroid test without autoimmunity assessment. White race and higher thyrotropin level were linked to increased odds of starting treatment, indicating potential disparities and the influence of SCH severity on decision-making.
Catheter-Directed Thrombolysis in the Management of Thrombotic Peripheral Artery Occlusions—Acute and Mid-Term Clinical Outcomes
Authors: Beschorner Ulrich , Boehme Tanja , Noory Elias , Bollenbacher Roaa , Salm Jonas , Mashayekhi Kambis , Westermann Dirk , Zeller Thomas
Published: September 2024

Objectives: To evaluate the safety and efficacy of catheter-directed thrombolysis (CDT) with the recombinant tissue plasminogen activator (rt-PA) in all patients with symptomatic peripheral artery disease in real world practice. Methods: Consecutive patients treated with CDT between January 2013 and December 2020 were included in this retrospective analysis. The primary endpoint was the rate of serious adverse events (SAEs) until discharge. Secondary endpoints included interventional success, predictors for SAEs, bleeding and reperfusion edema/compartment syndrome, limb salvage, and clinical outcomes including target lesion revascularization rate (TLR). Results: Overall, 1238 patients were treated with CDT. SAEs occurred in 511 (41.3%) of the patients, 314 (25.4%) being bleeding complications. There were 95 cases of reperfusion edema/compartment syndrome. Forty-two patients underwent amputation and 33 patients (2.7%) died. CDT was successful in 1177 cases (95.1%). Multivariate logistic regression analysis identified age, abciximab and alprostadil usage, and lysis duration as predictors for SAEs and the use of abciximab as a predictor of reperfusion edema/compartment syndrome. Predictors for bleeding were age, alprostadil usage, and lysis duration. At 12 and 24 months, the limb salvage rate was 91.6% and 88.8%, and TLR rate was 46% and 57.2%, respectively. Conclusions: CDT is an effective endovascular method for the treatment of thrombotic peripheral artery occlusions but is associated with a high complication rate. For SAEs in general and bleeding specifically, increasing age, alprostadil use, and lysis duration were independent risk factors.
Relationship between Preoperative Red Cell Distribution Width and Prolonged Postoperative Use of Catecholamines in Minimally Invasive Mitral Valve Surgery Patients: A Retrospective Cohort Study
Authors: Carrara Alfonso , Peluso Lorenzo , Baccanelli Federica , Parrinello Matteo , Santarpino Giuseppe , Giroletti Laura , Graniero Ascanio , Agnino Alfonso , Albano Giovanni
Published: September 2024

Background/Objectives: Elevated RDW has emerged in cardiac surgery as a potential means of preoperative risk stratification with the capacity to predict short- and long-term postoperative mortality, acute kidney injury, and postoperative atrial fibrillation. The question as to whether perioperative hemodynamic instability may be predicted by such a marker remains a topic of ongoing debate. The aim of this study was to explore the relationship between preoperative RDW and prolonged postoperative catecholamine use in minimally invasive mitral valve surgery. Methods: We performed a retrospective monocentric cohort study in an academic hospital; we enrolled patients who had undergone minimally invasive mitral valve surgery (including both robot-assisted and non-robot-assisted procedures) between January 2019 and December 2022. We considered the use of inotropes and/or vasopressors for at least twelve hours after post-surgery ICU admission to qualify as the prolonged postoperative use of catecholamines (PPUC). The RDW was obtained from the routine full blood count analysis performed upon admission or a maximum of 72 h before surgery. We also performed a multivariable logistic regression analysis with PPUC as the dependent variable. Results: We finally enrolled 343 patients. Upon multivariate analysis, RDW >14.4% was independently associated with prolonged postoperative catecholamine use when compared to the reference group (OR 2.62 [1.06–4.84]; p = 0.03). Moreover, the EuroSCORE II score (OR 1.38 [1.03–1.85]; p = 0.03), the cross-clamp time (OR 1.01 [1.01–1.02]; p < 0.01), and robot-assisted mitral valve surgery (OR 0.53 [0.30–0.93]; p < 0.03) were independently associated with the prolonged postoperative use of catecholamines. Conclusions: This study identified that an elevated preoperative RDW (>14.4%), the EuroSCORE II score, and the cross-clamp time independently predict prolonged postoperative catecholamine use in minimally invasive mitral valve surgery patients. Conversely, the robot-assisted approach was associated with a smaller hemodynamic impairment.
Effects of a Physical Exercise Program on Young People and Adults with Autism Spectrum Disorder—A Study Protocol
Authors: Ferreira José Pedro , Campos Maria João , Ataíde Sofia
Published: September 2024

Background/Objectives: Physical exercise (PE) has been shown to have positive effects on the symptoms associated with autism spectrum disorder (ASD). However, there is still no consensus on the most appropriate PE intervention model. With this in mind, we developed a program with the aim of determining the effects of PE on physical fitness, with a view to applying it as a potential treatment. Methods: Using an experimental methodology, this research work will recruit 18 institutionalized young people and adults who will be allocated to one of two groups, namely (i) the youth training group and (ii) the adult training group, using low-cost materials. Both intervention groups will perform 90 min of training per session, twice a week, for 12 weeks. Evaluations will be carried out at baseline and month 3. The impact of the exercise program will be assessed based on the variables of anthropometry, body composition, cardiovascular response, and cardiorespiratory fitness. Results: The results of this study will contribute to the development of more effective strategies, prescription recommendations, and interventions as a guarantee in future programs of better and greater adherence to PE by institutionalized individuals with ASD. Conclusions: In addition, we intend to make the PE program available if it promotes positive effects in the target population.
Research on In Situ Observation Method of Plankton Based on Convolutional Neural Network
Authors: Yuan Chengzhi , He Zhongjie , Ning Chunlin , Wang Weimin , Zhao Jinkai , Yuan Guozheng , Li Chao
Published: September 2024

The marine ecosystem is one of the most extensive and abundant ecosystems on Earth. Marine plankton is an important component, and its abundance, number of species, and dominant species are regarded as important monitoring indicators. Aiming at the problems of low accuracy and high complexity in identifying plankton based on convolutional neural networks, this study proposes a lightweight identification algorithm for plankton images based on the improved MobileNetV2. Firstly, the network layer structure is extracted by redesigning features to balance the depth and width of the network to reduce the model parameters; secondly, the lightweight coordinate attention (CA) mechanism is introduced to strengthen the attention and extraction ability of key areas; in addition, the structure of the network classifier is optimized to improve the utilization efficiency of the model parameters. The results show that the model achieves a 95.46% accuracy and 94.48% recall in 12 kinds of images. Compared with the initial MobileNetV2, the parameters and calculation amount are reduced by 72.47% and 52.09%, respectively, and the reasoning time for a single image is 6.15 ms. The model realizes the accurate identification of plankton in situ under the premise of ensuring it is lightweight. Combining time information and depth data, it is of great significance for marine ecological environment monitoring and prediction to obtain the abundance of various plankton.
Modeling and Analysis of Resistance-Sensing Characteristics for Two-Way Shape Memory Alloy-Based Deep-Sea Actuators
Authors: Guo Jian , Pan Binbin , Cui Weicheng , Xiang Huiming
Published: September 2024

Deep-sea actuators based on shape memory alloys (SMAs) are an emerging frontier field of multidisciplinary crossover, and the resistive sensing characteristics are the basis for the drive control of SMA deep-sea actuators. The resistance and resistivity of SMAs are complex and highly dependent on temperature and stress, and there is no complete description of SMAs for extreme environments of high pressure, low temperature, and high salinity in the deep sea. In this study, the logistic function is introduced to improve the kinetic equation of phase transition, and the macromechanical model, the law of resistance, and the resistivity mixing rule are integrated to model and analyze the resistive self-awareness characteristics of two-way shape memory alloy deep-sea actuators. The complex coupling relationships among resistance, strain, stress, resistivity, and temperature under constant load conditions are investigated, and the validity of the resistance-sensing model is verified by the water bath cycling test. The results show that the predicted values of the model agree well with the measured values. The self-perceived relationship between the resistance and deformation of the two-way shape memory alloy can be effectively expressed, which provides theoretical model support for the design of memory alloy deep sea actuators and sensorless drive control.
Focusing Monochromatic Water Surface Waves by Manipulating the Phases Using Submerged Blocks
Authors: Chung Fei Fang , Ong Muk Chen , Wang Jiyong
Published: September 2024

Focusing water surface waves is a promising approach for enhancing wave power in clean energy harvesting. This study presents a novel method that simplifies the wave-scattering problems of large-scale three-dimensional (3D) focusing blocks by decomposing them into scattering problems of two-dimensional (2D) phase regulators. The phase lags of transmitted waves over such 2D structures of various heights and thicknesses are investigated using both linear potential flow theory and numerical simulations based on smoothed-particle hydrodynamics (SPH). Due to propagation path differences of a converging wave, our approach compensates for circular phase differences within a maximal collection angle by optimizing the geometries of 2D phase regulators. Based on this concept, we designed three types of submerged structures and tested them in a 3D numerical water tank. All three structures successfully converted monochromatic plane waves into circular waves, which then converged at the designated focal point. This study offers a potential method to enhance the collection efficiency of monochromatic and regular waves for wave energy converters.
On the Conservation of the Canarian Laurel Forest: What Do Lichens Have to Say?
Authors: González-Montelongo Cristina , Pérez-Vargas Israel
Published: September 2024

The fragmentation and degradation of primary forests are serious threats to the long-term persistence not only of the tree species they comprise, but also of many organisms inhabiting them. The Canarian laurel forest, known as monteverde, is a highly threatened endemic forest of the Macaronesian region. Lichens are considered ideal bioindicators for assessing the effects of human disturbances on ecosystems and anticipating the response of other less sensitive organisms. However, no studies have used them as model organisms to analyze the conservation status of this primary forest in the Macaronesian region. In the present study, we analyzed several variables of the lichen biota of the Canarian laurel forest on the islands with the highest representation within this archipelago: La Gomera, La Palma, and Tenerife. We analyzed the species richness (and its relationship to island size with the real and potential vegetation areas of the laurel forest); the lichen diversity value, the number of shared and exclusive species on each of the islands as well as lichen functional traits as they have become important for evaluating the response of epiphytic lichens to environmental changes. The results indicate that there are signs of a potential extinction debt occurring in the diversity of epiphytic lichens in some areas. Furthermore, it has been observed that, despite the presence of some exclusive species on each island, the overall composition does not differ between them. Considering the functional traits of lichens, there are patterns that can provide information about the unique characteristics of the laurel forest of each of the studied islands.
Changes in Ergosterol Biosynthesis Alter the Response to Cycloheximide, 4-Nitroquinoline-N-Oxide, Weak Organic Acids, and Virulence in Candida glabrata
Authors: Eliaš Daniel , Tóth Hervay Nora , Černáková Lucia , Gbelská Yvetta
Published: September 2024

The ERG6 gene encodes the sterol C24-methyltransferase converting zymosterol to fecosterol in the ergosterol biosynthetic pathway. Here, we extend the results of functional analysis of the CgERG6 gene, which was previously shown to modulate drug susceptibility in Candida glabrata mutant cells, by demonstrating that its deletion leads to increased susceptibility to cycloheximide, 4-nitroquinoline-N-oxide and weak organic acids, and such effects are associated with attenuated virulence. Together with abrogated efflux of drug substrates by CgCdr1p and CgPdr12p, the Cgerg6Δ mutation leads to reduced cell surface hydrophobicity and decreased virulence of the mutant cells of C. glabrata. The absence of CgErg6p impacts the lipid organization and function of the plasma membrane, resulting in non-specific permeability and abrogation of normal function of membrane-bound proteins accompanied by decreased virulence in Cgerg6Δ cells. Galleria mellonella larvae were used as a non-vertebrate animal host model to determine differences in the virulence potential of C. glabrata strains (parental strain and the Cgerg6Δ deletion mutant). We found that Cgerg6Δ mutant strain attenuated in virulence caused 25–30% survival of larvae compared with parental strain.
Molecular Characterization of MDR and XDR Clinical Strains from a Tertiary Care Center in North India by Whole Genome Sequence Analysis
Authors: Tayyaba Uzma , Khan Shariq Wadood , Sultan Asfia , Khan Fatima , Akhtar Anees , Nagaraj Geetha , Ahmed Shariq , Bhattacharya Bhaswati
Published: September 2024

Whole genome sequencing (WGS) has the potential to greatly enhance AMR (Anti-microbial Resistance) surveillance. To characterize the prevalent pathogens and dissemination of various AMR-genes, 73 clinical isolates were obtained from blood and respiratory tract specimens, were characterized phenotypically by VITEK-2 (bioMerieux), and 23 selected isolates were genotypically characterized by WGS (Illumina). AST revealed high levels of resistance with 50.7% XDR, 32.9% MDR, and 16.4% non-MDR phenotype. A total of 11 K. pneumoniae revealed six sequence types, six K-locus, and four O-locus types, with ST437, KL36, and O4 being predominant types, respectively. They carried ESBL genes CTX-M-15 (90.9%), TEM-1D (72.7%), SHV-11 (54.5%), SHV-1, SHV-28, OXA-1, FONA-5, and SFO-1; NDM-5 (72.7%) and 63.6%OXA48-like carbapenamases; 90.9%OMP mutation; dfrA12, sul-1, ermB, mphA, qnrB1, gyrA831, and pmrB1 for other groups. Virulence gene found were Yerisiniabactin (90.9%), aerobactin, RmpADC, and rmpA2. Predominant plasmid replicons were Col(pHAD28), IncFII, IncFIB(pQil), and Col440. A total of seven XDR A. baumannii showed single MLST type(2) and single O-locus type(OCL-1); with multiple AMR-genes: blaADC-73, blaOXA-66, blaOXA-23, blaNDM-1, gyrA, mphE, msrE, and tetB. Both S. aureus tested were found to be ST22, SCCmec IVa(2B), and spa type t309; multiple AMR-genes: blaZ, mecA, dfrC, ermC, and aacA-aphD. Non-MDR Enterococcus faecalis sequenced was ST 946, with multiple virulence genes. This study documents for the first-time prevalent virulence genes and MLST types, along with resistance genes circulating in our center.
Clinical Application of 3D-Printed Artificial Vertebral Body (3DP AVB): A Review
Authors: Kiselev Roman , Zheravin Aleksander
Published: September 2024

Introduction: The choice of prosthesis for vertebral body reconstruction (VBR) remains a controversial issue due to the lack of a reliable solution. The subsidence rate of the most commonly used titanium mesh cages (TMC) ranges from 42.5% to 79.7%. This problem is primarily caused by the differences in the elastic modulus between the TMC and bone. This review aims to summarize the clinical and radiological outcomes of new 3D-printed artificial vertebral bodies (3DP AVB). Methods: A literature search of PubMed, Scopus and Google Scholar was conducted to extract relevant studies. After screening the titles and abstracts, a total of 50 articles were selected for full-text analysis. Results: Preliminary data suggest fewer implant-related complications with 3DP AVB. Most comparative studies indicate significantly lower subsidence rates, reduced operation times and decreased intraoperative blood loss. However, the scarcity of randomized clinical trials and the high variability of the results warrant caution. Conclusion: Most literature data show an advantage of 3DP AVB in terms of the operation time, intraoperative blood loss and subsidence rate. However, long manufacturing times, high costs and regulatory issues are this technology’s main drawbacks.
Integrating Ecosystem Services and Health into Landscape Functional Zoning: A Case Study of the Jinan Southern Mountainous Area, China
Authors: Li Kai , Hou Ying , Xin Ruhong , Rong Yuejing , Pan Xiang , Gao Zihan , Wang Ting , Lyu Bingyang , Guo Baimeng , Wang Haocheng , Li Xi
Published: September 2024

Ecosystems and their services to society have exhibited dramatic degradation all over the world, and landscape planning based on ecosystem service (ES) science is a promising way to mitigate ES loss and improve human well-being. However, ecosystem health, which is crucial for intrinsic ecosystem values, may be overlooked in ES-based landscape planning. Therefore, we proposed a landscape functional zoning method by combining the ES and EH using the Jinan Southern Mountainous Area as a case study. Specifically, we first quantified and mapped six ESs (including regulating, cultural, and provisioning services) and three EH properties (ecosystem vigor, organization, and resilience). Then, we used coupling coordination analysis to determine the coordination of the ES and EH, and adopted bundle analysis to reveal ES-EH compositions. Finally, landscape functional zones were delineated by spatially overlapping the maps of ES-EH bundle types and coupling coordination degrees. The results show that the different ESs and EH properties exhibited uneven spatial distributions. In terms of the ES-EH coupling coordination degree, high values were located along the mountains and aggregated in the eastern part of the study area, and the Caishi town had the highest coupling coordination degree on the town scale. Furthermore, five ES-EH bundle types were identified, i.e., bundles of multifunctionality, subordinate multifunctionality, the highest crop production, ESs and EH properties of medium levels, and ESs and EH properties of low levels. Finally, nine landscape functional zones based on the ES-EH bundle and coupling coordination were identified, and the grids within one zone were homogeneous in terms of their ES-EH compositions and coupling coordination. This study can contribute to the integration of ES and EH into landscape planning and provides a zoning method as a spatial instrument to achieve synergic ES-EH management.
Exploring Farmers’ Perspectives on Ecosystem Degradation and Restoration in Southern Hilly Regions of China
Authors: You Chang , Jiang Chunqian , Liu En
Published: September 2024

Ecosystem degradation has become a significant global environmental issue. Particularly in the south hilly regions, the necessity for ecological restoration is increasingly evident. This study takes Shiyan City in Hubei Province as an example and adopts a combination of extensive questionnaire surveys and field interviews to deeply analyze farmers’ perceptions of the factors influencing ecosystem degradation and restoration. This research fills a gap in current studies at the local level. The results show that (1) farmers generally believe that government policy support and natural resource protection are crucial in ecological restoration, with an average score of 4.4, indicating a strong willingness to participate. (2) There is a significant positive relationship among human consciousness, social governance, and government regulation. Human consciousness not only directly enhances the level of social governance but also promotes the effectiveness of government regulation through social governance. (3) The positive impact between economic benefits and the green economy is confirmed, indicating that ecological restoration is not only an environmental protection requirement but also creates economic development opportunities for farmers. This study emphasizes the importance of constructing a multi-party participation strategy for ecological restoration. Policymakers should focus on the combination of environmental education, policy support, and economic incentives to stimulate farmers’ enthusiasm and achieve sustainable ecosystem management. This research not only provides a theoretical basis for local ecological restoration practices but also offers an important reference for promoting regional sustainable development. By emphasizing the win–win relationship between ecological restoration and economic development, this study provides important new perspectives for the design and implementation of future ecological policies.
Hypoxia Modulates Sodium Chloride Co-transporter via CaMKII-β Pathway: An In Vitro Study with mDCT15 Cells
Authors: Liang Lijuan , Ueda Kohei , Ogura Sayoko , Shimosawa Tatsuo
Published: September 2024

Background: Hypoxia plays a crucial role in regulating various cellular functions, including ion-transport mechanisms in the kidney. The sodium-chloride co-transporter (NCC) is essential for sodium reabsorption in the distal convoluted tubule (DCT). However, the effects of hypoxia on NCC expression and its regulatory pathways remain unclear. We aimed to explore the effects and potential mechanisms of hypoxia on NCC in vitro. Methods: mDCT15 cells were treated with cobalt chloride (CoCl2) at a concentration of 300 μmol/L to induce hypoxia. The cells were harvested at different time points, namely 30 min, 1 h, 6 h, and 24 h, and the expression of NCC and CaMKII-β was analyzed using Western blot. Results: A time-dependent upregulation of NCC and CaMKII-β expression in response to CoCl2-induced hypoxia. KN93 reversed the effect of CoCl2 on NCC and phosphorylated NCC expression. Conclusions: Hypoxia, mediated through cobalt chloride treatment, upregulates NCC expression via the CaMKII-β pathway in mDCT15 cells.
Mortality Impact of Severe COVID-19 in the ICU: A Study from the Târgu Mureș Support Unit
Authors: Szederjesi Janos , Săplăcan Irina , Petrișor Marius , Șerdean Alexandra-Maria , Grigorescu Bianca-Liana
Published: September 2024

(1) Background: Since the onset of the COVID-19 pandemic, it has been recognized that a considerable proportion of critically ill patients may die of this disease. The current study aims to assess the overall 1-year outcomes within the UMFST COVID-19 Unit, providing valuable insights into the efficacy of specialized care facilities in managing severe cases of COVID-19. (2) Methods: This is a retrospective monocentric observational study including 294 patients confirmed to have SARS-CoV-2 infection. Demographic data and clinical and paraclinical parameters were assessed. Survival probabilities were estimated using Kaplan–Meier curves. (3) Results: Overall, the 1-year mortality was 89.4%. All deaths occurred in-hospital, with two patients dying after 28 days. Diabetes mellitus, chronic kidney failure, cerebrovascular disease, and atrial fibrillation were more prevalent in deceased patients. Thirty percent of patients needed endotracheal intubation during the first 24 h. The incidence of hospital-acquired pneumonia was higher among deceased patients. The SOFA score was significantly different between deceased vs. survivors. The survival analysis showed that the use of noradrenaline increased the likelihood of surviving COVID-19. (4) Conclusions: The severe comorbidities of the patients were the primary factors contributing to the increased mortality rate in the COVID-19 unit.
In Situ Measurement of Grease Capacitive Film Thickness in Bearings: A Review
Authors: Dai Wei
Published: September 2024

The majority of bearings in the world are lubricated by grease, and nearly 80% of premature bearing damage is attributed to lubrication issues. Accurate measurement and prediction of film thickness are crucial aspects of understanding the lubrication mechanism in grease-lubricated bearings. This work focuses on grease film thickness measurement using the capacitance method in real bearings. It comprehensively reviews the current status, identifies key challenges, and proposes solutions. Mechanisms of mainstream electronic components in capacitance measurement were reviewed for the first time. It enables more accurate capacitance measurement. A new capacitive model and electric network to measure film thickness in fully flooded, starved, and mixed regimes are developed. It is more comprehensive compared to current models. Classic dielectric models are reviewed, and suitable ones for lubricants are proposed. It facilitates a more precise film thickness measurement. Finally, a new grease film thickness model (bearing raceway) is proposed based on the 113 literature capacitive film thickness data points from five different authors. The satisfied R-squared value indicates a strong correlation.
Synthesis and Characterization of N-Nitroso-3-morpholinosydnonimine as NO Radical Donor
Authors: Saffon-Merceron Nathalie , Lherbet Christian , Hoffmann Pascal
Published: September 2024

N-Nitroso-3-morpholinosydnonimine 3 was prepared by nitrosation of SIN-1 and characterized by 1H-NMR, 13C-NMR, and HRMS. Its structure, confirmed by single crystal X-ray diffraction analysis, was found to be in agreement with its mesoionic and aromatic character. Unlike 3-morpholino-sydnonimine (SIN-1), which releases both nitric oxide and superoxide radical, decomposition of this nitrosylated sydonimine could yield nitric oxide as the only decomposition product, and thus without the formation of toxic peroxynitrite.