bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Efficacy and Safety of Tezepelumab and Other Biologics in Patients with Inadequately Controlled Asthma According to Thresholds of Type 2 Inflammatory Biomarkers: A Systematic Review and Network Meta-Analysis
Authors: Ando Koichi , Fukuda Yosuke , Tanaka Akihiko , Sagara Hironori
Published: February 2022

The anti-thymic stromal lymphopoietin antibody (tezepelumab) has therapeutical potential for inadequately controlled asthma. However, evidence comparing tezepelumab with other biologics is scarce. To address this issue, we performed a network meta-analysis to compare and rank the efficacy of five treatments (tezepelumab, dupilumab, benralizumab, mepolizumab, and placebo) in overall participants and in subgroups stratified by the thresholds of type 2 inflammatory biomarkers, including peripheral blood eosinophil count (PBEC) and fractional exhaled nitric oxide (FeNO). The primary endpoints were annualized exacerbation rate (AER) and any adverse events (AAEs). In the ranking assessment using surface under the cumulative ranking curve (SUCRA) of AER, tezepelumab ranked the highest overall and across subgroups (based on PBEC and FeNO level thresholds). A significant difference was observed between tezepelumab and dupilumab in the patient subgroup with PBEC < 150, and between tezepelumab and benralizumab in overall participants and the patient subgroup with PBEC ≥ 300 and ≥150, respectively. There was no significant difference in the incidence of AAEs in the overall participants between each pair of five treatment arms. These results provide a basis for the development of treatment strategies for asthma and may guide basic, clinical, or translational research.
Zinc Donor–Acceptor Schiff Base Complexes as Thermally Activated Delayed Fluorescence Emitters
Authors: Russegger Andreas , Eiber Lisa , Steinegger Andreas , Borisov Sergey M.
Published: February 2022

Four new zinc(II) Schiff base complexes with carbazole electron donor units and either a 2,3-pyrazinedicarbonitrile or a phthalonitrile acceptor unit were synthesized. The donor units are equipped with two bulky 2-ethylhexyl alkyl chains to increase the solubility of the complexes in organic solvents. Furthermore, the effect of an additional phenyl linker between donor and acceptor unit on the photophysical properties was investigated. Apart from prompt fluorescence, the Schiff base complexes show thermally activated delayed fluorescence (TADF) with quantum yields up to 47%. The dyes bearing a phthalonitrile acceptor emit in the green–yellow part of the electromagnetic spectrum and those with the stronger 2,3-pyrazinedicarbonitrile acceptor—in the orange–red part of the spectrum. The emission quantum yields decrease upon substitution of phthalonitrile with 2,3-pyrazinedicarbonitrile and upon introduction of the phenyl spacer. The TADF decay times vary between 130 µs and 3.5 ms at ambient temperature. The weaker phthalonitrile acceptor and the additional phenyl linker favor longer TADF decay times. All the complexes show highly temperature-dependent TADF decay time (temperature coefficients above −3%/K at ambient conditions) which makes them potentially suitable for application as molecular thermometers. Immobilized into cell-penetrating RL-100 nanoparticles, the best representative shows temperature coefficients of −5.4%/K at 25 °C that makes the material interesting for further application in intracellular imaging.
Structural Changes in Primary Teeth of Diabetic Children: Composition and Ultrastructure Analysis
Authors: Syed Sadatullah , Yassin Syed M. , Almalki Abdulrahman Yahya , Ali Salma Abubaker Abbas , Alqarni Abdulaziz M. Maken , Moadi Yousef M. , Alkhaldi Abdulrahman Masoud , Alqahtani Nasser M. , Hosmani Jagadish , Heboyan Artak , Patil Shankargouda
Published: February 2022

Diabetes affects the developing enamel by altering the mineralization process, which can have a detrimental effect on oral health. The objectives of this study were to examine the ultrastructure and composition of surface enamel in primary teeth of diabetic children and its clinical implications. Hundred extracted primary teeth from diabetic children (Test group: n = 50) and healthy children (Control group: n = 50), between 6 and 12 years of age, were subjected to scanning electron microscopy to qualitatively examine the enamel surface. Energy dispersive X-ray (EDX) analysis was performed to investigate the mass percentage of calcium (Ca) and phosphorous (P) in the surface enamel. Ultrastructural aberrations of surface enamel were observed in the test group teeth. Additionally, prism perforations were seen at the junction of rod and inter-rod enamel and the prisms were loosely packed. An even aprismatic layer of surface enamel was evident in the control group teeth. There was a statistically significant difference (p < 0.05) of Ca and P mass percentage between the test and control group teeth. The mean mass percentage rates of Ca and P were 33.75% and 16.76%, respectively. A poor surface characteristic and elemental composition of the enamel surface of primary teeth is observed in diabetic children. Therefore, appropriate caries preventive measures are mandatory to maintain the structural integrity of the tooth in these patients.
Managing Wound Healing with a High-Risk Patient: A Case Report
Authors: Potekaev Nikolai N. , Borzykh Olga B. , Medvedev German V. , Petrova Marina M. , Karpova Elena I. , Zatolokina Maria A. , Al-Zamil Mustafa , Demina Olga M. , Narodova Ekaterina A. , Shnayder Natalia A.
Published: February 2022

Wound healing is a complex, multi-step process. This process begins immediately after skin damage. The outcome of wound healing depends on the quality of each stage of this process: a normal or pathological scar. Violation of wound healing entails a decrease in the function of scar tissue as well as aesthetic dissatisfaction with the patient. This problem is especially important in aesthetic surgery. Patients who have come for beauty feel frustration, obtaining pathological scars. We have been dealing with the problem of wound healing after plastic surgery for about 10 years. Our approach includes the assessment of the risk of pathological wound healing and the treatment of high-risk patients. The risk assessment includes historical data on wound healing, signs of connective tissue dysfunction (especially patients with connective tissue dysplasia), and genetic polymorphisms of genes responsible for the structure of the components of the extracellular matrix of the skin. In the future, patients with a high risk of pathological scarring can be prescribed treatment after surgery. This article presents a clinical case in which we demonstrate our approach.
How the Strength of Monsoon Winds Shape Forest Dynamics
Authors: Ku Chen-Chia , Chao Kuo-Jung , Song Guo-Zhang M. , Lin Huan-Yu , Fan Su-Wei , Chao Wei-Chun
Published: February 2022

Monitoring long-term forest dynamics can improve our understanding of how tree species change under varying environmental conditions over time. The Nanjenshan Nature Reserve, located in the southernmost part of Taiwan, is covered by natural tropical forest and affected by the northeast monsoon wind in the winter. To explore how monsoon wind shapes the structure of vegetation, we used the long-term data of the Lanjenchi plot (high wind exposure forest) and Nanjenshan plot I (low wind exposure forest) located in the Nanjenshan Nature Reserve. In these two plots, all free-standing trees with diameter at breast height (DBH) ≥ 1 cm were identified, measured, and mapped five times in thirty years (1989–2019). Temporal changes of tree abundance, dominance, recruitment, and mortality were calculated for all species. We recorded 9694 ± 3924 individual ha−1 belonging to 126 species in the high wind exposure forest and 4019 ± 1791 individual ha−1 belonging to 105 species in the low wind exposure forest in the latest census (census 5). From 1989 to 2019, stem density in the high wind exposure forests decreased (from 12,179 ± 6689 to 9694 ± 3924 individual ha−1), while stem density in the low wind exposure forests increased (from 3253 ± 1208 to 4019 ± 1791 individual ha−1). The stem density of the rare species, which survived only in the high wind exposure forest, decreased over time. However, the basal areas of these two forests barely changed during the study periods. Due to environmental conditions, the two forests have developed different forest structures in spaces and time. Species stem density in the high wind exposure forest and diversity could continually decrease and affect the maintenance of species diversity in the monsoon wind-shaped forest.
A Case Building Ciliate in the Genus Pseudoblepharisma Found in Subtropical Fresh Water
Authors: Hines Hunter N. , McCarthy Peter J. , Esteban Genoveva F.
Published: February 2022

The genus Pseudoblepharisma is currently comprised of only one species, P. tenue, and one variant, P. tenue var. viride, both described as free-living ciliates thriving in oxygen depleted freshwater habitats of Europe. Here we report on this genus being discovered from subtropical freshwaters of Florida, USA. The Florida strain diverges from the P. tenue by being much larger, as well as having a far higher density of intracellular symbiotic green algae, resembling P. tenue var. viride. Unlike its European counterparts, the North American strain was observed to build a lorica, likely useful for protection and feeding; this has not been previously described for this genus. In contrast to P. tenue, the Florida strain does not have endosymbiotic purple bacteria. Despite large distances between sampling zones, the species is a close match at a morphological level to Pseudoblepharisma tenue var. viride. We provide the first 18S rRNA gene sequence for this species, allowing future investigations into the biogeography of this genus. As intensive sampling efforts continue to increase, cryptic microbial species will continue to be recorded from diverse freshwater habitats at a global scale.
Detection and Location of Multi-Period Phenomena in Chaotic Binary Sequences
Authors: Fan Chunlei , Ding Qun
Published: February 2022

Due to the influence of finite calculation accuracy and binary quantization method, the performance of chaotic binary sequences has been degraded in varying degrees, and some sequences emerge as multi-period phenomena. Aiming at the problem that it is difficult to accurately detect this phenomenon, this paper proposes a multi-period positioning algorithm (MPPA), which can accurately detect and locate the accurate period and local period phenomena contained in chaotic binary sequences. In order to test the effectiveness and correctness of the algorithm, the multi-period characteristics of logistic binary sequences with different calculation accuracy are analyzed. MPPA evaluates the randomness of binary sequences from a new perspective, which provides a new idea for the analysis of cryptographic security of chaotic sequences.
Ferrocene-Based Porous Organic Polymer (FPOP): Synthesis, Characterization and an Electrochemical Study
Authors: Petrovski Željko , Moreira Mateus P. , Santos Andreia F. M. , Freitas Sunny K. S. , Jordão Noémi , Maia Renata A. , Nunes Ana V. M. , Branco Luis C. , Cruz Hugo , Esteves Pierre M.
Published: February 2022

Ferrocene-based porous organic polymers (FPOPs) were prepared from phenol-formaldehyde polymer (Bakelite) and phenol as starting materials; and two possible mechanisms for polymerization were discussed. Solid-state 13C CP-MAS NMR, FTIR, powder XRD, elemental analysis and ICP (Fe, Na, B) were performed to characterize the prepared materials. The two synthetic approaches produced polymers with different pore sizes: the FPOP synthesized through Bakelite presented a higher surface area (52 m2 g−1) when compared to the one obtained by the bottom-up polymerization from phenol (only 5 m2 g−1). Thermogravimetric analysis confirmed the thermal stability of the material, which decomposed at 350 °C. Furthermore, cyclic voltammetry (CV) of the new FPOP on modified electrodes, in ACN and 0.1 M TBAP as an electrolyte, showed fully reversible electron transfer, which is similar to that observed for the ferrocene probe dissolved in the same electrolyte. As a proof-of-concept for an electrochromic device, this novel material was also tested, with a color change detected between yellow/brownish coloration (reduced form) and green/blue coloration (oxidized form). The new hybrid FPOP seems very promising for material science, energy storage and electrochromic applications, as well as for plastic degradation.
RF Acquisition System Based on μTCA for Testing of High-Gradient Acceleration Cavities
Authors: Menéndez Abraham , Esperante Daniel , García-Olcina Raimundo , Torres José , Pérez-Soler Joaquín , Marco Ricardo , Gimeno Benito , Martos Julio , Soret Jesús
Published: February 2022

The radio frequency (RF) laboratory hosted in the Corpuscular Physics Institute (IFIC) of the University of Valencia is designed to house a high-power and high-repetition-rate facility to test normal conduction RF accelerator cavities in the S-Band (2.9985 GHz) in order to perform R&D activities related to particle accelerator cavities. The system, which manages the entire process of RF signal generation, data acquisition and closed-loop control of the laboratory, is currently based on a modular and compact PXI platform system. This contribution details the development of a platform with similar features, but which is based on open architecture standards at both the hardware and software level. For this purpose, a complete system based on the μTCA platform has been developed. This new system must be able to work with accelerator cavities at other operating frequencies, such as 750 MHz, as well as to explore different options at firmware and software levels based on open-source codes.
MACRS: An Enhanced Directory-Based Resource Sharing Framework for Mobile Ad Hoc Networks
Authors: Khan Noreen , Haider Shahab , Baker Thar , Abbas Ghulam , Abbas Ziaul Haq
Published: February 2022

Recent technological developments have caused a rapid increase in the use of portable devices around the globe. However, these devices comprise limited processing resources that restrict their performance. To overcome this issue, the existing literature provides several frameworks that enable resource sharing through ad hoc clouds. However, these frameworks lack the ability to cater to the omni-directional movements of devices, which adversely affects the cloud stability, thereby, restricting the resource sharing process. To this end, this paper proposes a novel framework, namely Mobility-aware Ad hoc Cloud-based Resource Sharing (MACRS), which aims to enhance resource sharing among devices. To achieve this aim, MACRS proposes a new mobility-aware clustering algorithm that improves cloud stability. Moreover, the proposed framework prevents unfair resource exploitation and introduces an enhanced technique to handle emergency tasks. Furthermore, we employed event-triggered energy valuations’ synchronization, instead of periodic updates, which minimizes network congestion, hence staving off bandwidth wastage. Additionally, MACRS proposes to maintain the local directory at each node, instead of the cellular service provider, to reduce end-to-end delay during energy valuations’ verification and to minimize the overall execution time of tasks. The simulation results demonstrated that MACRS provides considerably improved cloud stability and resource sharing in comparison with eminent frameworks.
A Novel GPS Meaconing Spoofing Detection Technique Based on Improved Ratio Combined with Carrier-to-Noise Moving Variance
Authors: Zhu Xuefen , Lu Zhengpeng , Hua Teng , Yang Fan , Tu Gangyi , Chen Xiyuan
Published: February 2022

The Global Navigation Satellite System (GNSS) becomes vulnerable in a challenging environment, among which spoofing is the most dangerous threat. Meaconing, as the most convenient way to conduct spoofing, is widely studied around the world, and also leads to lots of research into corresponding anti-spoofing techniques. This paper develops a semi-hardware meaconing platform and proposes a novel GPS meaconing spoofing detection method based on Improved Ratio combined with Carrier-to-noise Moving variance (C/N0 − MV). The effectiveness has been validated theoretically and experimentally. The proposed method is proven useful when the meaconing signal has 5 dB power gain over the authentic signal, presenting 98% detection rate whereas the classic Signal quality monitoring (SQM) method with the Ratio metric presents only 30%.
A Novel Smart Charging Method to Mitigate Voltage Fluctuation at Fast Charging Stations
Authors: Alshareef Sami
Published: February 2022

The research presented in this paper focuses on the impact of fast charging stations (FCSs) on voltage quality. When the operation of FCSs causes a voltage fluctuation and light flicker, the FCSs may be disconnected, as per the utility general standard practice, which results in financial loss represented by FCS downtime. FCS downtime can be avoided by mitigating voltage fluctuation and light flicker. Flicker mitigation devices that are available in the market are characterized by their high total annual equivalent costs. As an alternative, a novel smart charging method is proposed in this study in order to mitigate both voltage fluctuation and light flicker, whereby customers can select one of three charging services available in fast chargers: premium, regular, or economic charging power. The charging power is selected according to customer priority in relation to time and cost, which offers more flexibility than those currently available in the literature. For instance, the premium power can be selected if the time is more valuable to the customer at the time of arrival at the FCS; in contrast, the regular or economic power are utilized if the cost is more valuable than the time. The results reveal that when an FCS charges a vehicle by an uncontrolled charging method, the FCS violates the flicker tolerance especially when demand for its service is increased by 20% and beyond. In contrast, the flicker limit is not violated when vehicles are charged from an FCS as per the proposed smart charging approach, even when the penetration on the FCS is increased by 50%. The proposed smart charging method offers a compromise solution to satisfy several stakeholders with different interests. Thus, the system operator equipment, FCS investors, nearby customers, and owners of electric vehicles will not be impacted by integrating the FCSs into the distribution networks.
Spatiotemporal Evolution and Influencing Factors of Electricity Consumption in the Yangtze River Delta Region
Authors: Lv Tao , Pi Duyang , Deng Xu , Hou Xiaoran , Xu Jie , Wang Liya
Published: February 2022

Electricity consumption accounts for a considerable part of the final energy consumption, and it is important for economic development and human life. This study explores the spatiotemporal evolution characteristics and influencing factors of electricity consumption in the Yangtze River Delta region in China from 2006 to 2019, using the gravity model and Logarithmic Mean Divisia Index method, respectively. The results show that: (1) The centers of gravity for the total final, industrial and residential electricity consumptions have a trend of migration towards the west. (2) The distance of migration of the center of gravity for residential electricity consumption is the highest, and the trend of migration of the center of gravity for industrial and total final electricity consumptions are synchronous. (3) Economic development is the main reason for the growth in regional electricity consumption, and the decrease in the investment electricity consumption intensity inhibits the growth of electricity consumption. This study provides references to restrain the excessive increase in electricity consumption and improve the layout of power facilities at the regional level.
Experimental Investigation of Hydrous Ethanol Gasoline on Engine Noise, Cyclic Variations and Combustion Characteristics
Authors: Chen Zhenbin , Deng Jiaojun , Zhen Haisheng , Wang Chenyu , Wang Li
Published: February 2022

Nowadays, the noise pollution of internal combustion engines is a very important factor influencing human health and is the main noise source of urban environmental noise. Additionally, the main source of gasoline engine noise consists of combustion noise in the cylinder, where the combustion noise is influenced by the combustion processes within the combustion chamber, especially the cyclic variation in the engine combustion. Thus, the inter-relationship between engine noise, cyclic variation and combustion is of great interest to be explored. Moreover, despite the environmental advantages of clean energy, the impact of different fuels on the internal combustion engine’s noise emissions cannot be ignored. As a result, in this work, three blends were prepared and used as test fuels, namely pure gasoline (E0), 10% hydrous ethanol (E10W) and 20% hydrous ethanol (E20W) by volume, accompanied by engine operating at a steady speed of 2000 r/min under various loads. The experimental results show that lower engine noise was observed for both E10W and E20W compared to E0. Upon the addition of hydrous ethanol, the peak in-cylinder pressure increased while the maximum pressure rise rate ((dp/dφ)max) decreased at the low and medium loads. Furthermore, the coefficients of variation in indicated mean effective pressure (COVimep) and COV(dp/dφ)max for the two blended fuels were higher than those for pure gasoline. Compared with those of E20W, E10W has lower COVimep and COV(dp/dφ)max at low and medium loads. The (dp/dφ)max and noise emission have a positive relation when the engine is fueled with the hydrous ethanol–gasoline blends, whereas the cyclic variation parameters vary in the opposite direction of the noise emission level for all the blend mixtures. Moreover, (dp/dφ)max has an essential effect on the combustion noise from a gasoline engine.
Cytoprotective Effects of Lactobacilli on Mouse Epithelial Cells during Salmonella Infection
Authors: Zhang Guangzhi , Raheem Abdul , Gao Xintao , Zhang Jianwei , Shi Lijun , Wang Mingyan , Li Ming , Yin Yajie , Li Shaohan , Cui Xiaodong , Yan Xinlei , Yue Min , Wen Hefei , Qin Tong
Published: February 2022

Treatment of common pathogens, such as Salmonella species, Escherichia coli, Staphylococcus aureus, etc., is a big challenge for a practitioner. Antibiotics’ side effects during their application for the treatment of infectious diseases should not be underestimated as they have many issues, such as the transfer of antibiotics-resistant genes, dysbiosis, and antibiotic-resistant strains, which is the main hurdle in the eradication of diseases. To avoid these antibiotics complications, in modern countries, the interest of using probiotics in feed supplementation to promote health and prevent or treat intestinal infectious diseases has been increasing. The purpose of the present study was to evaluate the probiotic potential of three Lactobacilli strains isolated from clinically healthy dogs for their further utilization as a dietary supplement for dogs to avoid pathogenic and antibiotic complication. After 16SrRNA sequencing, in vitro tests were conducted to assess the survival potential of Lactobacilli under simulated gastrointestinal conditions and adhesion ability to the MODE-K cell line, effects on epithelial barrier function, anti-inflammatory activities, effects on host defensin peptides (beta-defensin 3), and inhibitory effects on common pathogens. Lactobacilli showed considerable potential to survive in simulated gastrointestinal environmental conditions, low pH, and high bile salt concentrations along with good adhesion properties with MODE-K cells. Pathogenic bacterial growth and their adhesion to MODE-K cells were significantly inhibited by Lactobacilli. Real-time PCR analyses further demonstrated that the L. acidophilus strain AR1 and AR3 inhibit Salmonella-induced proinflammatory cytokine (IL-6, IL-8, IL-1β) production and reinforce the expression of tight junction protein (occludin). None of the strains induce mRNA expression of beta-defensin 3 in MODE-K cells. Based on the in vitro results, the L. acidophilus strain AR1 has the potential to be supplemented in canine feed. However, further in vivo studies investigating health-promoting effects are awaited.
Physical Model of a Single Bubble Growth during Nucleate Pool Boiling
Authors: Voglar Jure
Published: February 2022

A simplified physical model of a single bubble growth during nucleate pool boiling was developed. The model was able to correlate the experimentally observed data of the bubble’s growth time and its radius evolution with the use of the appropriate input parameters. The calculated values of separated heat fluxes from the heater wall, thermal boundary layer, and to the bulk liquid gave us a new insight into the complex mechanisms of the nucleate pool boiling process. The thermal boundary layer was found to supply the majority of the heat to the growing bubble. The heat flux from the thermal boundary layer to the bubble was found to be close to the Zuber’s critical heat flux limit (890 kW/m2). This heat flux was substantially larger than the input heater wall heat flux of 50 kW/m2. The thermal boundary layer acts as a reservoir of energy to be released to the growing bubble, which is filled during the waiting time of the bubble growth cycle. Therefore, the thickness of the thermal boundary layer was found to have a major effect on the bubble’s growth time.
Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine
Authors: Rzeszotek Sylwia , Trybek Grzegorz , Tarnowski Maciej , Serwin Karol , Jaroń Aleksandra , Schneider Gabriela , Kolasa Agnieszka , Wiszniewska Barbara
Published: February 2022

The intestinal wall and epithelial cells are interconnected by numerous intercellular junctions. Colostrum (Col), in its natural form, is a secretion of the mammary gland of mammals at the end of pregnancy and up to 72 h after birth. Recently, it has been used as a biologically active dietary supplement with a high content of lactoferrin (Lf). Lf, a glycoprotein with a broad spectrum of activity, is becoming more popular in health-promoting supplements. This study aims to investigate whether Col supplementation can affect small and large intestine morphology by modulating the expression of selected proteins involved in tissue integrity. We examined the thickness of the epithelium, and the length of the microvilli, and assessed the expression of CDH1, CDH2, CTNNB, CX43, VCL, OCLN, HP, MYH9, and ACTG2 gene levels using qRT-PCR and at the protein level using IHC. Additionally, to evaluate whether the effect of Col supplementation is temporary or persistent, we performed all analyses on tissues collected from animals receiving Col for 1, 3, or 6 months. We noticed a decrease in CDH1 and CDH2 expression, especially after 3 months of supplementation in the large intestine and in CTNNB in the small intestine as well as increased levels of CX43 and CTNNB1 in the small intestine. The present data indicate that Col can temporarily alter some components of the cell adhesion molecules involved in the formation of the cellular barrier.
Nutrients against Glucocorticoid-Induced Muscle Atrophy
Authors: Lee Min-Kyeong , Jeong Hyeon Hak , Kim Myeong-Jin , Ryu Heeyeon , Baek Jiwon , Lee Bonggi
Published: February 2022

Glucocorticoid excess is a critical factor contributing to muscle atrophy. Both endogenous and exogenous glucocorticoids negatively affect the preservation of muscle mass and function. To date, the most effective intervention to prevent muscle atrophy is to apply a mechanical load in the form of resistance exercise. However, glucocorticoid-induced skeletal muscle atrophy easily causes fatigue in daily physical activities, such as climbing stairs and walking at a brisk pace, and reduces body movements to cause a decreased ability to perform physical activity. Therefore, providing adequate nutrients in these circumstances is a key factor in limiting muscle wasting and improving muscle mass recovery. The present review will provide an up-to-date review of the effects of various nutrients, including amino acids such as branched-chain amino acids (BCAAs) and β–hydroxy β–methylbutyrate (HMB), fatty acids such as omega-3, and vitamins and their derivates on the prevention and improvement of glucocorticoid-induced muscle atrophy.
Characteristic Volatiles and Cultivar Classification in 35 Apple Varieties: A Case Study of Two Harvest Years
Authors: Wu Xinye , Bi Jinfeng , Fauconnier Marie-Laure
Published: February 2022

A large number of apple varieties (35) from the same germplasm were investigated over two consecutive harvest years. A total of 39 volatile compounds were identified by HS-SPME-GC-MS, and quantified by external standards. Principal component analysis was applied to study the relationship between varieties and volatiles. To obtain better discrimination, 23 of 35 apple varieties were classified into four cultivars and good classification could be observed by partial least squares discriminant analysis. Ethyl 2-methylbutyrate, 2-methyl-1-butanol, Z-3-hexenyl acetate, E-2-hexen-1-ol, linalool and dodecanol were the most important variables to discriminate apple cultivars. Based on the volatile concentration and thresholds, ethyl 2-methylbutyrate, hexanal, 1-hexanol, E-2-nonenal and linalool were the critical characterized odor-active compounds among 35 apple varieties over two harvest years. From the present work, seasonal effects greatly influenced the formation of volatiles.
Market Integration and Price Dynamics under Market Shocks in European Union Internal and External Cheese Export Markets
Authors: Xue Huidan , Wang Liming , Li Chenguang
Published: February 2022

The dairy sector in the European Union (EU) has experienced policy changes and market shocks recently. Using the global vector autoregressive (GVAR) model, this paper explores regional market integration, the feedback between market shocks and price dynamics, and the link between EU’s cheese export markets and energy market. This paper assesses and compares which influencing factors are typically associated with intra-EU and extra-EU cheese export price movement with regards to shocks to crude oil price, farm-gate raw milk price, and consumer price index (CPI) for food and cheese production of six representative EU member states, respectively. Using generalized impulse response functions, this paper finds that EU’s internal cheese export market is not well integrated, while EU’s external market is well integrated, with France as an exception. It also finds that the external cheese export market is vulnerable to shocks from the energy market compared to the internal market. Raw milk prices from the upstream supply chain have strong spill-over effects on EU’s internal cheese export market, yet their impact on extra-EU cheese export prices is relatively less significant. The movement patterns of extra-EU cheese export prices of Ireland and the UK show similar patterns in the long run. It is concluded that the dynamics of cheese export prices in the internal and external markets of the EU are different under market shocks.
Recovery of Functional Proteins from Pig Brain Using pH-Shift Processes
Authors: Chanted Jaruwan , Panpipat Worawan , Cheong Ling-Zhi , Chaijan Manat
Published: February 2022

The goal of this work is to explore if pH-shift processing could be used as a cold refinery technique to manufacture pig brain protein isolate (PI). Pig brain protein had the highest solubility at pH 2 (acid method) and pH 12 (alkaline method). As the protein solution’s zeta-potential was near 0 with the lowest solubility, pH 5.0 was chosen as the precipitation pH. Alkaline process produced a 32% dry matter yield with phospholipid content of 35 mg/100 g. The alkaline-made PI was better at forming soft gels and had good emulsifying and foaming capabilities. Although the acid-made PI included less residual lipid and total haem protein and was whiter in colour, it could not be gelled. Acid-made PI was more prone to lipid oxidation with a poorer ability to function as an emulsifier and foaming agent. Thus, functional proteins from pig brain may be isolated using the alkaline pH-shift technique.
Effect of Ginkgo Biloba Powder on the Physicochemical Properties and Quality Characteristics of Wheat Dough and Fresh Wet Noodles
Authors: Li Liangyi , Zhou Wenhua , Wu Anqi , Qian Xin , Xie Le , Zhou Xiaojie , Zhang Lin
Published: February 2022

Effects of ginkgo biloba powder (GBP) on the chemical, physicochemical properties and quality of dough and fresh wet noodles were investigated. Lower contents of gluten and starch, and higher contents of fibre, amylose and flavonoids in GBP than wheat flour, were detected. Water absorption of dough increased and the development time and stability time of dough were decreased with GBP addition. Meanwhile, the pasting properties results showed that the addition of GBP reduced the aging degree of starch and improved the thermal stability of dough. Scanning electron microscopy results showed that addition of GBP smoothed the surface of raw noodles while increasing the hole size of the cooked noodles. With increased GBP addition (0~40%), the chewiness and extensibility of the fresh wet noodles increased significantly (p < 0.05), and the sensory scores changed, ascending from 0~20% substitution, and then descending from 20~40% substitution. The digestibility and estimated glycemic index (eGI) values of the GBP fresh wet noodles decreased significantly (p < 0.05). In general, 20% GBP addition could improve the chewiness, extensibility, taste and nutrition of fresh wet noodles, and decrease the digestibility and eGI values of noodles. Thus, GBP has potential for application in the noodle industry.
Edible Coatings from Opuntia ficus-indica Cladodes Alongside Chitosan on Quality and Antioxidants in Cherries during Storage
Authors: Christopoulos Miltiadis V. , Gkatzos Dimitrios , Kafkaletou Mina , Bai Jinhe , Fanourakis Dimitrios , Tsaniklidis Giorgos , Tsantili Eleni
Published: February 2022

The aim of this work was to investigate the effect of edible coatings (ECs) prepared from extracts of Opuntia ficus-indica (OFI) cladodes in comparison with a commercial chitosan formulation on the quality of ‘Regina’ cherries packaged in macro-perforated bags and stored for up to 28 d (1 °C, 90% RH). The coating concentrations were 25% and 50% aqueous OFI extract (approximately 0.59 and 1.18% dry matter, respectively), 1% OFI alcohol insoluble polysaccharide and 1% chitosan. The variables evaluated included weight loss (WL), respiration rates (RR), peel color, firmness, microbial decay, total antioxidants (phenolics, flavonoids, anthocyanins, antioxidant capacity), individual phenolic compounds (anthocyanins, hydroxycinnamic acids, flavan-3-O-ols), and pedicel removal force. The main results show that all coatings reduced WL and RR similarly, enhanced firmness throughout storage and antioxidants after 28 d of storage compared to the controls. Among treatments, chitosan resulted in much higher peel glossiness and firmness in comparison to OFI extracts. On day 28, all ECs resulted in higher antioxidants than controls, OFI extracts resulted in higher cyaniding-3-O-rutinoside than chitosan, while 50% OFI treatment resulted in the highest catechin concentration. Therefore, OFI extracts are promising ECs for cherry storage since they exhibited no negative effect, improved quality and extended storage life by one week compared to the controls.
Study on the Effect of Key Genes ME2 and adhE during Luzhou-Flavor Baijiu Brewing
Authors: Zhou Wen , Xia Yu , Zhao Yajiao , Wang Yan , Wu Zhengyun , Suyama Taikei , Zhang Wenxue
Published: February 2022

Luzhou-flavor baijiu (LFB) is brewed by the combined action of various microorganisms, and its flavor is affected by the microbial community and the genes they express, but which genes are the key ones during LFB brewing is less clear. Based on our previous studies the genes ME2 and adhE were identified as key genes, but which role they play was also unknown. In this study functional microorganisms were screened based on the key genes ME2 and adhE, and they were identified to be Rummeliibacillus suwonensis, Clostridium tyrobutyricum and Lactobacillus buchneri. Then simulated fermentation experiments were carried out with the functional microorganisms, and during the fermentation process expression of the key genes and the amounts of the main flavors were detected to analyze the role of the key genes. The results showed that the key gene ME2 was significantly positively correlated with the contents of the main acids, however the key gene adhE and the formation of the main esters in the LFB brewing process was a significant positive correlation. This study verified the two key genes ME2 and adhE complement each other in the LFB brewing process, playing an important role in promoting the formation of flavor substances, and are very beneficial to improve the quality of LFB.
Limosilactobacillus fermentum ING8, a Potential Multifunctional Non-Starter Strain with Relevant Technological Properties and Antimicrobial Activity
Authors: Pakroo Shadi , Tarrah Armin , Takur Rohit , Wu Manyu , Corich Viviana , Giacomini Alessio
Published: February 2022

Lactic acid bacteria (LAB) have gained particular attention among different exopolysaccharide-producing microorganisms due to their safety status and effects on human health and food production. Exopolysaccharide-producing LAB play a crucial role in different ways, such as improving texture, mouthfeel, controlling viscosity, and for low-calorie food production. In this study, we isolated a multifunctional strain with good exopolysaccharide production properties. Limosilactobacillus fermentum ING8 was isolated from an Indian traditional fermented milk (Dahi) and evaluated for its safety, enzymatic activity, NaCl resistance and temperature tolerance, milk coagulation, and storage stability. Finally, the complete genome of this strain was sequenced and subjected to safety in silico evaluation and genomic analysis. The results revealed that L. fermentum ING8 possesses relevant technological properties, such as exopolysaccharide production, antimicrobial activity, and galactose utilization. Besides, this strain showed very high stability to storage conditions at refrigeration temperature. In addition, the genomic analysis did not evidence any possible deleterious elements, such as acquired antibiotic resistance genes, virulence genes, or hemolysis-related genes. However, all structural genes related to the galactose operon and EPS production were detected. Therefore, L. fermentum ING8 can be considered a promising multifunctional bacterium to be proposed as non-starter in different types of dairy productions.
Physicochemical and Sensory Characteristics of Sausages Made with Grasshopper (Sphenarium purpurascens) Flour
Authors: Cruz-López Salvador O. , Álvarez-Cisneros Yenizey M. , Domínguez-Soberanes Julieta , Escalona-Buendía Héctor B. , Sánchez Claudia N.
Published: February 2022

Insects are currently of interest due to their high nutritional value, in particular for the high concentration of quality protein. Moreover, it can also be used as an extender or binder in meat products. The objective was to evaluate grasshopper flour (GF) as a partial or total replacement for potato starch to increase the protein content of sausages and achieve good acceptability by consumers. GF has 48% moisture, 6.7% fat and 45% total protein. Sausages were analyzed by NIR and formulations with GF in all concentrations (10, 7, 5 and 3%) combined with starch (3, 5 and 7%) increased protein content. Results obtained for the sausages formulations with grasshoppers showed an increase in hardness, springiness, gumminess and chewiness through a Texture-Profile-Analysis. Moreover, a* and b* are similar to the control, but L* decreased. The check-all-that-apply test showed the attributes highlighted for sausages with GF possessed herbal flavor, brown color, and granular texture. The liking-product-landscape map showed that the incorporation of 7 and 10% of GF had an overall liking of 3.2 and 3.3, respectively, considered as “do not like much”. GF can be used as a binder in meat products up to 10% substitution. However, it is important to improve the overall liking of the sausage.
A Scaled Boundary Finite-Element Method with B-Differentiable Equations for 3D Frictional Contact Problems
Authors: Xue Binghan , Du Xueming , Wang Jing , Yu Xiang
Published: February 2022

Contact problems are among the most difficult issues in mathematics and are of crucial practical importance in engineering applications. This paper presents a scaled boundary finite-element method with B-differentiable equations for 3D frictional contact problems with small deformation in elastostatics. Only the boundaries of the contact system are discretized into surface elements by the scaled boundary finite-element method. The dimension of the contact system is reduced by one. The frictional contact conditions are formulated as B-differentiable equations. The B-differentiable Newton method is used to solve the governing equation of 3D frictional contact problems. The convergence of the B-differentiable Newton method is proven by the theory of mathematical programming. The two-block contact problem and the multiblock contact problem verify the effectiveness of the proposed method for 3D frictional contact problems. The arch-dam transverse joint contact problem shows that the proposed method can solve practical engineering problems. Numerical examples show that the proposed method is a feasible and effective solution for frictional contact problems.
The Preparation and Properties of Composite Hydrogels Based on Gelatin and (3-Aminopropyl) Trimethoxysilane Grafted Cellulose Nanocrystals Covalently Linked with Microbial Transglutaminase
Authors: Zhao Shouwei , Chen Zhiwei , Dong Yaqi , Lu Wenhui , Zhu Deyi
Published: February 2022

Mechanically enhanced gelatin-based composite hydrogels were developed in the presence of functionalized cellulose nanocrystals (CNCs) employing microbial transglutaminase (mTG) as a binding agent. In this work, the surfaces of CNCs were grafted with (3-Aminopropyl) trimethoxysilane with a NH2 functional group, and the success of CNCs’ modification was verified by FTIR spectroscopy and XPS. The higher degree of modification in CNCs resulted in more covalent cross-linking and dispersibility within the gelatin matrix; thus, the as-prepared hydrogels showed significantly improved mechanical properties and thermo-stability, as revealed by dynamic rheological analysis, uniaxial compression tests and SEM. The biocompatibility of the obtained hydrogels was evaluated by the MTT method, and it was found that the grafted CNCs had no obvious inhibitory effect on cell proliferation. Hence, the mechanically enhanced gelatin-based hydrogels might have great potential in biomedical applications.
Study of Several Alginate-Based Hydrogels for In Vitro 3D Cell Cultures
Authors: Jiao Weijie , Li Xiaohong , Shan Jingxin , Wang Xiaohong
Published: February 2022

Hydrogel, a special system of polymer solutions, can be obtained through the physical/chemical/enzymic crosslinking of polymer chains in a water-based dispersion medium. Different compositions and crosslinking methods endow hydrogel with diverse physicochemical properties. Those hydrogels with suitable physicochemical properties hold manifold functions in biomedical fields, such as cell transplantation, tissue engineering, organ manufacturing, drug releasing and pathological model analysis. In this study, several alginate-based composite hydrogels, including gelatin/alginate (G-A), gelatin/alginate/agarose (G-A-A), fibrinogen/alginate (F-A), fibrinogen/alginate/agarose (F-A-A) and control alginate (A) and alginate/agarose (A-A), were constructed. We researched the advantages and disadvantages of these hydrogels in terms of their microscopic structure (cell living space), water holding capacity, swelling rate, swelling–erosion ratio, mechanical properties and biocompatibility. Briefly, alginate-based hydrogels can be used for three-dimensional (3D) cell culture alone. However, when mixed with other natural polymers in different proportions, a relatively stable network with a good cytocompatibility, mechanical strength and water holding capacity can be formed. The physical and chemical properties of the hydrogels can be adjusted by changing the composition, proportion and cross-linking methods of the polymers. Conclusively, the G-A-A and F-A-A hydrogels are the best hydrogels for the in vitro 3D cell cultures and pathological model construction.
Bioinspired Sandcastle Worm-Derived Peptide-Based Hybrid Hydrogel for Promoting the Formation of Liver Spheroids
Authors: Chen Yu-Hsu , Ku Yuan-Hao , Wang Kuo-Cheng , Chiang Hung-Chi , Hsu Yu-Pao , Cheng Ming-Te , Wang Ching-Shuen , Wee Yinshen
Published: February 2022

The generation of hepatic spheroids is beneficial for a variety of potential applications, including drug development, disease modeling, transplantation, and regenerative medicine. Natural hydrogels are obtained from tissues and have been widely used to promote the growth, differentiation, and retention of specific functionalities of hepatocytes. However, relying on natural hydrogels for the generation of hepatic spheroids, which have batch to batch variations, may in turn limit the previously mentioned potential applications. For this reason, we researched a way to establish a three-dimensional (3D) culture system that more closely mimics the interaction between hepatocytes and their surrounding microenvironments, thereby potentially offering a more promising and suitable system for drug development, disease modeling, transplantation, and regenerative medicine. Here, we developed self-assembling and bioactive hybrid hydrogels to support the generation and growth of hepatic spheroids. Our hybrid hydrogels (PC4/Cultrex) inspired by the sandcastle worm, an Arg-Gly-Asp (RGD) cell adhesion sequence, and bioactive molecules derived from Cultrex BME (Basement Membrane Extract). By performing optimizations to the design, the PC4/Cultrex hybrid hydrogels can enhance HepG2 cells to form spheroids and express their molecular signatures (e.g., Cyp3A4, Cyp7a1, A1at, Afp, Ck7, Ck1, and E-cad). Our study demonstrated that this hybrid hydrogel system offers potential advantages for hepatocytes in proliferating, differentiating, and self-organizing to form hepatic spheroids in a more controllable and reproducible manner. In addition, it is a versatile and cost-effective method for 3D tissue cultures in mass quantities. Importantly, we demonstrate that it is feasible to adapt a bioinspired approach to design biomaterials for 3D culture systems, which accelerates the design of novel peptide structures and broadens our research choices on peptide-based hydrogels.
Exercise for Trismus Prevention in Patients with Head and Neck Cancer: A Network Meta-Analysis of Randomized Controlled Trials
Authors: Wang Ya-Hui , Huang Yi-Ai , Chen I-Hui , Hou Wen-Hsuan , Kang Yi-No
Published: February 2022

A common side effect of managing head and neck cancer is trismus, which devastates patients’ quality of life. The purpose of this study was to investigate prophylactic exercise interventions for preventing trismus and difficulty in mouth opening in head and neck cancer. Five databases were searched for randomized controlled trials. Network meta-analysis was performed with risk ratio (RR) or mean difference (MD) with 95% confidence interval (CI). This study finally included 11 randomized controlled trials (n = 805). Trismus risk in patients who received exercise with phone call follow up (E + P) was significantly lower than those received usual care (RR = 0.42; 95% CI: 0.29 to 0.61) and exercise alone (RR = 0.33; 95% CI: 0.18 to 6.22). Mouth opening in usual care was significantly lower than in the tri-integrated strategy group (MD = 15.22; 95% CI: 8.88 to 21.56). Exercise is recommended for preserving mouth opening distance in patients with head and neck cancer. Tri-integrated strategies could be an effective method for preventing trismus.