bluuebunny/crossref_metadata_2025 huggingface dataset:
Acoustic Emission for Determining Early Age Concrete Damage as an Important Indicator of Concrete Quality/Condition before Loading
Authors: Trąmpczyński Wiesław , Goszczyńska Barbara , Bacharz Magdalena
Published: August 2020

Phenomena occurring during the curing of concrete can decrease its mechanical properties, specifically strength, and serviceability, even before it is placed. This is due to excessive stresses caused by temperature gradients, moisture changes, and chemical processes arising during the concreting and in hardened concrete. At stress concentration sites, microcracks form in the interfacial transition zones (ITZ) in the early phase and propagate deeper into the cement paste or to the surface of the element. Microcracks can contribute to the development of larger cracks, reduce the durability of structures, limit their serviceability, and, in rare cases, lead to their failure. It is thus important to search for a tool that allows objective assessment of damage initiation and development in concrete. Objectivity of the assessment lies in it being independent of the constituents and additives used in the concrete or of external influences. The acoustic emission-based method presented in this paper allows damage detection and identification in the early age concrete (before loading) for different concrete compositions, curing conditions, temperature variations, and in reinforced concrete. As such, this method is an objective and effective tool for damage processes detection.
Challenges of Co–Cr Alloy Additive Manufacturing Methods in Dentistry—The Current State of Knowledge (Systematic Review)
Authors: Konieczny Bartłomiej , Szczesio-Wlodarczyk Agata , Sokolowski Jerzy , Bociong Kinga
Published: August 2020

Complex dental components which are individually tailored to the patient can be obtained due to new additive manufacturing technology. This paper reviews the metallic powders used in dental applications, the fabrication process (build orientation, process parameters) and post-processing processes (stress relieving, surface finishing). A review of the literature was performed using PubMed, ScienceDirect, Mendeley and Google Scholar. Over eighty articles were selected based on relevance to this review. This paper attempts to include the latest research from 2010 until 2020, however, older manuscripts (10 articles) were also selected. Over 1200 records were identified through the search; these were screened for title and/or summary. Over eighty articles were selected based on relevance to this review. In order to obtain a product which can be used in clinical applications, the appropriate manufacturing parameters should be selected. A discussion was made on optimal selective laser melting (SLM) parameters in dentistry. In addition, this paper includes a critical review of applied thermal treatment methods for Co–Cr alloys used in dentistry.
A State-of-the-Art Review on the Wear of the Occlusal Surfaces of Natural Teeth and Prosthetic Crowns
Authors: Branco Ana Catarina , Colaço Rogério , Figueiredo-Pina Célio Gabriel , Serro Ana Paula
Published: August 2020

This review focuses on the wear mechanisms of natural and restorative dental materials, presenting a comprehensive description and analysis of the works published in the last two decades on the wear at the interface of occlusal surfaces. Different groups of tribological pairs were considered: tooth-tooth, tooth-restorative material (tooth-ceramic, tooth-resin-based-materials, and tooth-metal), and restorative-restorative materials. The lack of standardization of the wear tests impairs the direct comparison of the obtained results. However, it was possible to infer about the main wear mechanisms observed on the different classes of dental materials. Concerning ceramics, their toughness and surface finishing determines the wear of antagonist tooth. Abrasion revealed to be the main wear mechanisms at occlusal interface. In the case of resin-based composites, the cohesion of the organic matrix and the nature, shape, and amount of filler particles greatly influences the dental wear. The protruding and detachment of the filler particles are the main causes of abrasion of antagonist enamel. Metallic materials induce lower wear on antagonist enamel than the other classes of materials, because of their low hardness and high ductility. Most of the studies revealed plastic deformation and adhesive wear as the main wear mechanisms. Overall, more research in this area is needed for a better understanding of the mechanisms involved at the occlusal surfaces wear. This would be essential for the development of more suitable restoration materials.
2D Digital Image Correlation and Region-Based Convolutional Neural Network in Monitoring and Evaluation of Surface Cracks in Concrete Structural Elements
Authors: Słoński Marek , Tekieli Marcin
Published: August 2020

This paper shows how 2D digital image correlation (2D DIC) and region-based convolutional neural network (R-CNN) can be combined for image-based automated monitoring and assessment of surface crack development of concrete structural elements during laboratory quasi-static tests. In the presented approach, the 2D DIC-based monitoring enables estimation of deformation fields on the surface of the concrete element and measurements of crack width. Moreover, the R-CNN model provides unmanned simultaneous detection and localization of multiple cracks in the images. The results show that the automatic monitoring and evaluation of crack development in concrete structural elements is possible with high accuracy and reliability.
Consequences of Different Mechanical Surface Preparation of Ni-Base Alloys during High Temperature Oxidation
Authors: J. Nowak Wojciech , Siemek Krzysztof , Ochał Kamil , Kościelniak Barbara , Wierzba Bartek
Published: August 2020

The influence of surface roughness on its high temperature oxidation for an Ni-base superalloy was studied using laser profilometry, atomic force microscopy, mass change measurements, glow-discharge optical emission spectrometry, scanning electron microscopy, X-ray diffraction, and positron annihilation methods. The isothermal and cyclic air oxidation tests were performed at 1000 °C and showed dependence of oxidation behavior on surface roughness. Smoother surfaces oxidation resulted in the formation of a multilayered oxide scale consisting of NiO, Cr2O3, and internally oxidized Al2O3 while a rougher surface formed protective Al2O3 scale. The factors responsible for different oxidation behaviors were determined as higher concentration of vacancies and increased residual stresses in the near-surface region of studied alloys.
The n–Si/p–CVD Diamond Heterojunction
Authors: Łoś Szymon , Paprocki Kazimierz , Szybowicz Mirosław , Fabisiak Kazimierz
Published: August 2020

Due to the possible applications, materials with a wide energy gap are becoming objects of interest for researchers and engineers. In this context, the polycrystalline diamond layers grown by CVD methods on silicon substrates seem to be a promising material for engineering sensing devices. The proper tuning of the deposition parameters allows us to develop the diamond layers with varying crystallinity and defect structure, as was shown by SEM and Raman spectroscopy investigations. The cathodoluminescence (CL) spectroscopy revealed defects located just in the middle of the energy gap of diamonds. The current–voltage–temperature, I−V−T characteristics performed in a broad temperature range of 77–500 K yielded useful information about the electrical conduction in this interesting material. The recorded I−V−T in the forward configuration of the n–Si/p–CVD diamond heterojunction indicated hopping trough defects as the primary mechanism limiting conduction properties. The Ohmic character of the carriers flux permitting throughout heterojunction is intensified by charges released from the depletion layer. The magnification amplitude depends on both the defect density and the probability that biasing voltage is higher than the potential barrier binding the charge. In the present work, a simple model is proposed that describes I−V−T characteristics in a wide range of voltage, even where the current saturation effect occurs.
Binary and Ternary 3D Nanobundles Metal Oxides Functionalized Carbon Xerogels as Electrocatalysts toward Oxygen Reduction Reaction
Authors: Abdelwahab Abdalla , Carrasco-Marín Francisco , Pérez-Cadenas Agustín F.
Published: August 2020

A series of carbon xerogels doped with cobalt, nickel, and iron have been prepared through the sol–gel method. The doped carbon xerogels were further functionalized with binary and ternary transition metal oxides containing Co, Ni, and Zn oxides by the hydrothermal method. A development in the mesopore volume is achieved for functionalized carbon xerogel doped with iron. However, in the functionalization of carbon xerogel with ternary metal oxides, a reduction in pore diameter and mesopore volume is found. In addition, all functionalized metal oxides/carbon are in the form of 3D nanobundles with different lengths and widths. The prepared samples have been tested as electrocatalysts for oxygen reduction reaction (ORR) in basic medium. All composites showed excellent oxygen reduction reaction activity; the low equivalent series resistance of the Zn–Ni–Co/Co–CX composite was especially remarkable, indicating high electronic conductivity. It has been established that the role of Zn in this type of metal oxides nanobundles-based ORR catalyst is not only positive, but its effect could be enhanced by the presence of Ni.
Stolarsky Means in Many Variables
Authors: Simić Slavko , Bin-Mohsin Bandar
Published: August 2020

We give in this article two possible explicit extensions of Stolarsky means to the multi-variable case. They attain all main properties of Stolarsky means and coincide with them in the case of two variables.
Study of Lotka–Volterra Biological or Chemical Oscillator Problem Using the Normalization Technique: Prediction of Time and Concentrations
Authors: Sánchez-Pérez Juan Francisco , Conesa Manuel , Alhama Iván , Cánovas Manuel
Published: August 2020

The normalization of dimensionless groups that rule the system of nonlinear coupled ordinary differential equations defined by the Lotka–Volterra biological or chemical oscillator has been derived in this work by applying a normalized nondimensionalization protocol. The normalization procedure, which is quite accurate, does not require complex mathematical steps; however, a deep physical understanding of the problem is required to choose the appropriate references to define the dimensionless variables. From the dimensionless groups derived, the functional dependences of some unknowns of interest are established. Due to the coupled nature of the problem that induces temporal concentration rates of each species that are quite different at each point of the phase diagram, this diagram has been divided into four stretches corresponding to the four quadrants. For each stretch, the limit values (maximum or minimum) of the variables, as well as their duration, are expressed in terms of the dimensionless groups derived before. Finally, to check all the mentioned dependences, a numerical simulation has been carried out.
A Unified Scalable Equivalent Formulation for Schatten Quasi-Norms
Authors: Shang Fanhua , Liu Yuanyuan , Shang Fanjie , Liu Hongying , Kong Lin , Jiao Licheng
Published: August 2020

The Schatten quasi-norm is an approximation of the rank, which is tighter than the nuclear norm. However, most Schatten quasi-norm minimization (SQNM) algorithms suffer from high computational cost to compute the singular value decomposition (SVD) of large matrices at each iteration. In this paper, we prove that for any p, p1, p2>0 satisfying 1/p=1/p1+1/p2, the Schatten p-(quasi-)norm of any matrix is equivalent to minimizing the product of the Schatten p1-(quasi-)norm and Schatten p2-(quasi-)norm of its two much smaller factor matrices. Then, we present and prove the equivalence between the product and its weighted sum formulations for two cases: p1=p2 and p1≠p2. In particular, when p>1/2, there is an equivalence between the Schatten p-quasi-norm of any matrix and the Schatten 2p-norms of its two factor matrices. We further extend the theoretical results of two factor matrices to the cases of three and more factor matrices, from which we can see that for any 0<p<1, the Schatten p-quasi-norm of any matrix is the minimization of the mean of the Schatten (⌊1/p⌋+1)p-norms of ⌊1/p⌋+1 factor matrices, where ⌊1/p⌋ denotes the largest integer not exceeding 1/p.
From Hahn–Banach Type Theorems to the Markov Moment Problem, Sandwich Theorems and Further Applications
Authors: Olteanu Octav
Published: August 2020

The aim of this review paper is to recall known solutions for two Markov moment problems, which can be formulated as Hahn–Banach extension theorems, in order to emphasize their relationship with the following problems: (1) pointing out a previously published sandwich theorem of the type f ≤ h ≤ g, where f, −g are convex functionals and h is an affine functional, over a finite-simplicial set X, and proving a topological version for this result; (2) characterizing isotonicity of convex operators over arbitrary convex cones; giving a sharp direct proof for one of the generalizations of Hahn–Banach theorem applied to the isotonicity; (3) extending inequalities assumed to be valid on a small subset, to the entire positive cone of the domain space, via Krein–Milman or Carathéodory’s theorem. Thus, we point out some earlier, as well as new applications of the Hahn–Banach type theorems, emphasizing the topological versions of these applications.
Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
Authors: Ma Yan , Li Jie , Zhang Xin-Yue , Ni Hao-Dong , Wang Feng-Biao , Wang Hai-Ying , Wang Zhi-Peng
Published: August 2020

Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.
Hip Fracture Prevention in Osteoporotic Elderly and Cancer Patients: An On-Line French Survey Evaluating Current Needs
Authors: Rodrigues Laëtitia , Cornelis François H. , Chevret Sylvie
Published: August 2020

Background and objectives: Hip fracture is a major public health issue. Those fractures lead to high costs and a decrease in quality of life. A national French survey was conducted, with the objectives to firstly assess the current management of hip fracture and its prevention, both in the osteoporotic and cancer settings, and secondly to evaluate the opinions of physicians on the potential use of minimally invasive implantable devices to prevent hip fracture in alternative of surgery. Materials and methods: This national survey was conducted in France between April and July 2017. Questionnaires were sent to orthopedic surgeons, interventional radiologists, oncologists, and rheumatologists. Completed questionnaires were analyzed and compared according to two indications: orthopedics-traumatology and oncology. Factors associated with these responses were assessed using univariable analyses, based on chi-square tests or an exact Fisher test, as appropriate. Results: A total of 182 questionnaires were completed and further analyzed. Physicians have highlighted the need for a low re-fracture rate and to improve life expectancy for more than 1 year (50% for responders of the orthopedics-traumatology questionnaire and 80% for the responders interested in both indications), as well as quality of life (12.5% and 31%, respectively), but with no significant differences in the oncologic indication. Most of the experts were willing to use or prescribe implantable devices for prevention (63% in orthopedics-traumatology and 93% in oncology), although limited clinical experience (54 and 58%) and surgical risk (around 30% in each indication) were considered as limits. Conclusions: Prevention of hip fracture remains a concern for physicians. More clinical experience with implantable devices, in particular in cancer patients, is needed, but implemented in a strategy to maximize patient recovery while reducing costs.
Polymyxins and Bacterial Membranes: A Review of Antibacterial Activity and Mechanisms of Resistance
Authors: Ayoub Moubareck Carole
Published: August 2020

Following their initial discovery in the 1940s, polymyxin antibiotics fell into disfavor due to their potential clinical toxicity, especially nephrotoxicity. However, the dry antibiotic development pipeline, together with the rising global prevalence of infections caused by multidrug-resistant (MDR) Gram-negative bacteria have both rejuvenated clinical interest in these polypeptide antibiotics. Parallel to the revival of their use, investigations into the mechanisms of action and resistance to polymyxins have intensified. With an initial known effect on biological membranes, research has uncovered the detailed molecular and chemical interactions that polymyxins have with Gram-negative outer membranes and lipopolysaccharide structure. In addition, genetic and epidemiological studies have revealed the basis of resistance to these agents. Nowadays, resistance to polymyxins in MDR Gram-negative pathogens is well elucidated, with chromosomal as well as plasmid-encoded, transferrable pathways. The aims of the current review are to highlight the important chemical, microbiological, and pharmacological properties of polymyxins, to discuss their mechanistic effects on bacterial membranes, and to revise the current knowledge about Gram-negative acquired resistance to these agents. Finally, recent research, directed towards new perspectives for improving these old agents utilized in the 21st century, to combat drug-resistant pathogens, is summarized.
Optimization of Ruthenium Dioxide Solid Contact in Ion-Selective Electrodes
Authors: Lenar Nikola , Paczosa-Bator Beata , Piech Robert
Published: August 2020

Ruthenium dioxide occurs in two morphologically varied structures: anhydrous and hydrous form; both of them were studied in the scope of this work and applied as mediation layers in ion-selective electrodes. The differences between the electrochemical properties of those two materials underlie their diverse structure and hydration properties, which was demonstrated in the paper. One of the main differences is the occurrence of structural water in RuO2•xH2O, which creates a large inner surface available for ion transport and was shown to be a favorable feature in the context of designing potentiometric sensors. Both materials were examined with SEM microscope, X-ray diffractometer, and contact angle microscope, and the results revealed that the hydrous form can be characterized as a porous structure with a smaller crystallite size and more hydrophobic properties contrary to the anhydrous form. Potentiometric and electrochemical tests carried out on designed GCD/RuO2/K+-ISM and GCD/RuO2•xH2O/K+-ISM electrodes proved that the loose porous microstructure with chemically bounded water, which is characteristic for the hydrous form, ensures the high electrical capacitance of electrodes (up to 1.2 mF) with consequently more stable potential (with the potential drift of 0.0015 mV/h) and a faster response (of a few seconds).
A Review of SLMed Magnesium Alloys: Processing, Properties, Alloying Elements and Postprocessing
Authors: Liu Shuai , Guo Hanjie
Published: August 2020

Selective laser melting (SLM) is an additive manufacturing method with rapid solidification properties, which is conducive to the preparation of alloys with fine microstructures and uniform chemical compositions. Magnesium alloys are lightweight materials that are widely used in the aerospace, biomedical and other fields due to their low density, high specific strength, and good biocompatibility. However, the poor laser formability of magnesium alloy restricts its application. This paper discusses the current research status both related to the theoretical understanding and technology applications. There are problems such as limited processable materials, immature process conditions and metallurgical defects on SLM processing magnesium alloys. Some efforts have been made to solve the above problems, such as adding alloy elements and applying postprocessing. However, the breakthroughs in these two areas are rarely reviewed. Due to the paucity of publications on postprocessing and alloy design of SLMed magnesium alloy powders, we review the current state of research and progress. Moreover, traditional preparation techniques of magnesium alloys are evaluated and related to the SLM process with a view to gaining useful insights, especially with respect to the postprocessing and alloy design of magnesium alloys. The paper also reviews the influence of process parameters on formability, densification and mechanical behavior of magnesium. In addition, the progress of microstructure and metallurgical defects encountered in the SLM processed parts is described. Finally, this article summarizes the research results, and with respect to materials and metallurgy, the new challenges and prospects in the SLM processing of magnesium alloy powders are proposed with respect to alloy design, base material purification, inclusion control and theoretical calculation, and the role of intermetallic compounds.
Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis
Authors: Tootsi Kaspar , Vilba Kadri , Märtson Aare , Kals Jaak , Paapstel Kaido , Zilmer Mihkel
Published: August 2020

Metabolomic analysis is an emerging new diagnostic tool, which holds great potential for improving the understanding of osteoarthritis (OA)-caused metabolomic shifts associated with systemic inflammation and oxidative stress. The main aim of the study was to map the changes of amino acid, biogenic amine and complex lipid profiles in severe OA, where the shifts should be more eminent compared with early stages. The fasting serum of 70 knee and hip OA patients and 82 controls was assessed via a targeted approach using the AbsoluteIDQ™ p180 kit. Changes in the serum levels of amino acids, sphingomyelins, phoshatidylcholines and lysophosphatidylcholines of the OA patients compared with controls suggest systemic inflammation in severe OA patients. Furthermore, the decreased spermine to spermidine ratio indicates excessive oxidative stress to be associated with OA. Serum arginine level was positively correlated with radiographic severity of OA, potentially linking inflammation through NO synthesis to OA. Further, the level of glycine was negatively associated with the severity of OA, which might refer to glycine deficiency in severe OA. The current study demonstrates significant changes in the amino acid, biogenic amine and low-molecular weight lipid profiles of severe OA and provides new insights into the complex interplay between chronic inflammation, oxidative stress and OA.
Branched-Chain Amino Acids and Branched-Chain Keto Acids in Hyperammonemic States: Metabolism and as Supplements
Authors: Holeček Milan
Published: August 2020

In hyperammonemic states, such as liver cirrhosis, urea cycle disorders, and strenuous exercise, the catabolism of branched-chain amino acids (BCAAs; leucine, isoleucine, and valine) is activated and BCAA concentrations decrease. In these conditions, BCAAs are recommended to improve mental functions, protein balance, and muscle performance. However, clinical trials have not demonstrated significant benefits of BCAA-containing supplements. It is hypothesized that, under hyperammonemic conditions, enhanced glutamine availability and decreased BCAA levels facilitate the amination of branched-chain keto acids (BCKAs; α-ketoisocaproate, α-keto-β-methylvalerate, and α-ketoisovalerate) to the corresponding BCAAs, and that BCKA supplementation may offer advantages over BCAAs. Studies examining the effects of ketoanalogues of amino acids have provided proof that subjects with hyperammonemia can effectively synthesize BCAAs from BCKAs. Unfortunately, the benefits of BCKA administration have not been clearly confirmed. The shortcoming of most reports is the use of mixtures intended for patients with renal insufficiency, which might be detrimental for patients with liver injury. It is concluded that (i) BCKA administration may decrease ammonia production, attenuate cataplerosis, correct amino acid imbalance, and improve protein balance and (ii) studies specifically investigating the effects of BCKA, without the interference of other ketoanalogues, are needed to complete the information essential for decisions regarding their suitability in hyperammonemic conditions.
Genomic Analysis of Three Cheese-Borne Pseudomonas lactis with Biofilm and Spoilage-Associated Behavior
Authors: Quintieri Laura , Caputo Leonardo , De Angelis Maria , Fanelli Francesca
Published: August 2020

Psychrotrophic pseudomonads cause spoilage of cold fresh cheeses and their shelf-life reduction. Three cheese-borne Pseudomonas sp., ITEM 17295, ITEM 17298, and ITEM 17299 strains, previously isolated from mozzarella cheese, revealed distinctive spoilage traits based on molecular determinants requiring further investigations. Genomic indexes (ANI, isDDH), MLST-based phylogeny of four housekeeping genes (16S rRNA, gyrB, rpoB and rpoD) and genome-based phylogeny reclassified them as Pseudomonas lactis. Each strain showed distinctive phenotypic traits at 15 and 30 °C: ITEM 17298 was the highest biofilm producer at both temperatures, whilst ITEM 17295 and ITEM 17299 showed the strongest proteolytic activity at 30 °C. A wider pattern of pigments was found for ITEM 17298, while ITEM 17295 colonies were not pigmented. Although the high genomic similarity, some relevant molecular differences supported this phenotypic diversity: ITEM 17295, producing low biofilm amount, missed the pel operon involved in EPS synthesis and the biofilm-related Toxin-Antitoxin systems (mqsR/mqsA, chpB/chpS); pvdS, required for the pyoverdine synthesis, was a truncated gene in ITEM 17295, harboring, instead, a second aprA involved in milk proteolysis. This work provided new insight into the food spoiler microbiota by identifying these mozzarella cheese spoilers as P. lactis; molecular targets to be exploited in the development of novel preservative strategies were also revealed.
Steel Slag Characterisation—Benefit of Coupling Chemical, Mineralogical and Magnetic Techniques
Authors: Herbelin Maud , Bascou Jérôme , Lavastre Véronique , Guillaume Damien , Benbakkar Mhammed , Peuble Steve , Baron Jean-Philippe
Published: August 2020

Steel-making slag is largely used today in road construction and other applications, but significant volumes are landfilled and cannot be recycled for excessive contents in hazardous metals, such as chromium or vanadium. The long-term behaviour of this material is still little known, and the characterisation of large volume slag dumps remains an environmental challenge. In this study various analytical techniques are used to characterise Electric Arc Furnace (EAF) slag landfilled for several decades and exposed to chemical weathering and erosion. Coupling chemical, mineralogical and magnetic techniques helps to understand the relations between hazardous metals and mineral phases. A special interest is given to Fe-bearing minerals microstructure so as to link the magnetic properties of the material to its mineralogical composition. The studied slag presents high amounts of chromium (between 1 and 3 wt. %) and very high magnetic susceptibility values (near 60 × 10−6 m3/kg), explained by the presence of magnetite and a spinel solid solution. Some correlations are found between magnetic susceptibility and potentially hazardous metals, providing new perspectives for future environmental investigations.
Catechins as Tools to Understand the Molecular Basis of Neurodegeneration
Authors: Martinez Pomier Karla , Ahmed Rashik , Melacini Giuseppe
Published: August 2020

Protein misfolding as well as the subsequent self-association and deposition of amyloid aggregates is implicated in the progression of several neurodegenerative disorders including Alzheimer’s and Parkinson’s diseases. Modulators of amyloidogenic aggregation serve as essential tools to dissect the underlying molecular mechanisms and may offer insight on potential therapeutic solutions. These modulators include green tea catechins, which are potent inhibitors of amyloid aggregation. Although catechins often exhibit poor pharmacokinetic properties and bioavailability, they are still essential tools for identifying the drivers of amyloid aggregation and for developing other aggregation modulators through structural mimicry. As an illustration of such strategies, here we review how catechins have been used to map the toxic surfaces of oligomeric amyloid-like species and develop catechin-based phenolic compounds with enhanced anti-amyloid activity.
Anti-Inflammatory Effect of Simonsinol on Lipopolysaccharide Stimulated RAW264.7 Cells through Inactivation of NF-κB Signaling Pathway
Authors: Li Lian-Chun , Pan Zheng-Hong , Ning De-Sheng , Fu Yu-Xia
Published: August 2020

Simonsinol is a natural sesqui-neolignan firstly isolated from the bark of Illicium simonsii. In this study, the anti-inflammatory activity of simonsinol was investigated with a lipopolysaccharide (LPS)-stimulated murine macrophages RAW264.7 cells model. The results demonstrated that simonsinol could antagonize the effect of LPS on morphological changes of RAW264.7 cells, and decrease the production of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in LPS-stimulated RAW264.7 cells, as determined by Griess assay and enzyme-linked immunosorbent assay (ELISA). Furthermore, simonsinol could downregulate transcription of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 as measured by reverse transcription polymerase chain reaction (RT-PCR), and inhibit phosphorylation of the alpha inhibitor of NF-κB (IκBα) as assayed by Western blot. In conclusion, these data demonstrate that simonsinol could inhibit inflammation response in LPS-stimulated RAW264.7 cells through the inactivation of the nuclear transcription factor kappa-B (NF-κB) signaling pathway.
Experimental Pretreatment with Chlorogenic Acid Prevents Transient Ischemia-Induced Cognitive Decline and Neuronal Damage in the Hippocampus through Anti-Oxidative and Anti-Inflammatory Effects
Authors: Lee Tae-Kyeong , Kang Il-Jun , Kim Bora , Sim Hye Jin , Kim Dae- Won , Ahn Ji Hyeon , Lee Jae-Chul , Ryoo Sungwoo , Shin Myoung Cheol , Cho Jun Hwi , Kim Young-Myeong , Park Joon Ha , Choi Soo Young , Won Moo-Ho
Published: August 2020

Chlorogenic acid (CGA), an ester of caffeic acid and quinic acid, is among the phenolic acid compounds which can be naturally found in green coffee extract and tea. CGA has been studied since it displays significant pharmacological properties. The aim of this study was to investigate the effects of CGA on cognitive function and neuroprotection including its mechanisms in the hippocampus following transient forebrain ischemia in gerbils. Memory and learning following the ischemia was investigated by eight-arm radial maze and passive avoidance tests. Neuroprotection was examined by immunohistochemistry for neuronal nuclei-specific protein and Fluoro-Jade B histofluorescence staining. For mechanisms of the neuroprotection, alterations in copper, zinc-superoxide dismutase (SOD1), SOD2 as antioxidant enzymes, dihydroethidium and 4-hydroxy-2-nonenal as indicators for oxidative stress, and anti-inflammatory cytokines (interleukin (IL)-4 and IL-13) and pro-inflammatory cytokines (tumor necrosis factor α (TNF-α) and IL-2) were examined by Western blotting and/or immunohistochemistry. As a result, pretreatment with 30 mg/kg CGA attenuated cognitive impairment and displayed a neuroprotective effect against transient forebrain ischemia (TFI). In Western blotting, the expression levels of SOD2 and IL-4 were increased due to pretreatment with CGA and, furthermore, 4-HNE production and IL-4 expressions were inhibited by CGA pretreatment. Additionally, pretreated CGA enhanced antioxidant enzymes and anti-inflammatory cytokines and, in contrast, attenuated oxidative stress and pro-inflammatory cytokine expression. Based on these results, we suggest that CGA can be a useful neuroprotective material against ischemia-reperfusion injury due to its antioxidant and anti-inflammatory efficacies.
Novel Methodologies for Chemical Activation in Organic Synthesis under Solvent-Free Reaction Conditions
Authors: Avila-Ortiz Claudia Gabriela , Juaristi Eusebio
Published: August 2020

One central challenge for XXI century chemists is the development of sustainable processes that do not represent a risk either to humanity or to the environment. In this regard, the search for more efficient and clean alternatives to achieve the chemical activation of molecules involved in chemical transformations has played a prominent role in recent years. The use of microwave or UV-Vis light irradiation, and mechanochemical activation is already widespread in many laboratories. Nevertheless, an additional condition to achieve “green” processes comes from the point of view of so-called atom economy. The removal of solvents from chemical reactions generally leads to cleaner, more efficient and more economical processes. This review presents several illustrative applications of the use of sustainable protocols in the synthesis of organic compounds under solvent-free reaction conditions.
Assessment of Protein Content and Phosphorylation Level in Synechocystis sp. PCC 6803 under Various Growth Conditions Using Quantitative Phosphoproteomic Analysis
Authors: Toyoshima Masakazu , Tokumaru Yuma , Matsuda Fumio , Shimizu Hiroshi
Published: August 2020

The photosynthetic apparatus and metabolic enzymes of cyanobacteria are subject to various controls, such as transcriptional regulation and post-translational modifications, to ensure that the entire cellular system functions optimally. In particular, phosphorylation plays key roles in many cellular controls such as enzyme activity, signal transduction, and photosynthetic apparatus restructuring. Therefore, elucidating the governing functions of phosphorylation is crucial to understanding the regulatory mechanisms underlying metabolism and photosynthesis. In this study, we determined protein content and phosphorylation levels to reveal the regulation of intracellular metabolism and photosynthesis in Synechocystis sp. PCC 6803; for this, we obtained quantitative data of proteins and their phosphorylated forms involved in photosynthesis and metabolism under various growth conditions (photoautotrophic, mixotrophic, heterotrophic, dark, and nitrogen-deprived conditions) using targeted proteomic and phosphoproteomic analyses with nano-liquid chromatography-triple quadrupole mass spectrometry. The results indicated that in addition to the regulation of protein expression, the regulation of phosphorylation levels of cyanobacterial photosynthetic apparatus and metabolic enzymes was pivotal for adapting to changing environmental conditions. Furthermore, reduced protein levels of CpcC and altered phosphorylation levels of CpcB, ApcA, OCP, and PsbV contributed to the cellular response of the photosynthesis apparatus to nitrogen deficiency.
A Leopard Cannot Change Its Spots: Unexpected Products from the Vilsmeier Reaction on 5,10,15-Tritolylcorrole
Authors: Caroleo Fabrizio , Petrella Greta , Di Zazzo Lorena , Nardis Sara , Berionni Berna Beatrice , Cicero Daniel O. , Paolesse Roberto
Published: August 2020

The reaction of 5,10,15-tritolylcorrole with 3-dimethylaminoacrolein (3-DMA) and POCl3 gives a further example of the rebel reactivity of this contracted macrocycle. While no evidence was obtained for the formation of the expected β-acrolein corrole, the inner core substituted N21,N22-3-formylpropylcorrole and the 10-acrolein isocorrole were the reaction products. By increasing the temperature or the amount of the Vilsmeier reagent, the 10-isocorrole became the unique reaction product. The formation of the isocorrole by electrophilic attack of the Vilsmeier reagent to the 10-position of the corrole is unprecedented in the porphyrinoids field and it could pave the way for a novel route to the preparation of stable isocorroles.
Synthesis, Crystal Structures and Anticancer Studies of Morpholinyldithiocarbamato Cu(II) and Zn(II) Complexes
Authors: Ajibade Peter A. , Andrew Fartisincha P. , Botha Nandipha L. , Solomane Nolwazi
Published: August 2020

Cu(II) and Zn(II) morpholinyldithiocarbamato complexes, formulated as [Cu(MphDTC)2] and [Zn(μ-MphDTC)2(MphDTC)2], where MphDTC is morpholinyldithiocarbamate were synthesized and characterized by elemental analysis, spectroscopic techniques and single-crystal X-ray crystallography. The molecular structure of the Cu(II) complex revealed a mononuclear compound in which the Cu(II) ion was bonded to two morpholinyl dithiocarbamate ligands to form a four-coordinate distorted square planar geometry. The molecular structure of the Zn(II) complex was revealed to be dinuclear, and each metal ion was bonded to two morpholinyl dithiocarbamate bidentate anions, one acting as chelating ligand, the other as a bridge between the two Zn(II) ions. The anticancer activity of the morpholinyldithiocarbamate ligand, Cu(II) and Zn(II) complexes were evaluated against renal (TK10), melanoma (UACC62) and breast (MCF7) cancer cells by a Sulforhodamine B (SRB) assay. Morpholinyldithiocarbamate was more active than the standard drug parthenolide against renal and breast cancer cell lines, and [Zn(μ-MphDTC)2(MphDTC)2] was the most active complex against breast cancer. The copper(II) complex had a comparable activity with the standard against renal and breast cancer cell lines but showed an enhanced potency against melanoma when compared to parthenolide.
Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs
Authors: Jia Min , E Zhongbo , Zhai Fei , Bing Xin
Published: August 2020

The excessive use or abuse of pesticides and veterinary drugs leads to residues in food, which can threaten human health. Therefore, there is an extremely urgent need for multi-analyte analysis techniques for the detection of pesticide and veterinary drug residues, which can be applied as screening techniques for food safety monitoring and detection. Recent developments related to rapid multi-residue detection methods for pesticide and veterinary drug residues are reviewed herein. Methods based on different recognition elements or the inherent characteristics of pesticides and veterinary drugs are described in detail. The preparation and application of three broadly specific recognition elements—antibodies, aptamers, and molecular imprinted polymers—are summarized. Furthermore, enzymatic inhibition-based sensors, near-infrared spectroscopy, and SERS spectroscopy based on the inherent characteristics are also discussed. The aim of this review is to provide a useful reference for the further development of rapid multi-analyte analysis of pesticide and veterinary drug residues.
Toxicity Assessment of SiO2 and TiO2 in Normal Colon Cells, In Vivo and in Human Colon Organoids
Authors: Park Sung Bum , Jung Won Hoon , Kim Ki Young , Koh Byumseok
Published: August 2020

We conducted systemic assessments on the toxicity of silicon dioxide (SiO2) and titanium dioxide (TiO2) nanoparticles using different forms of normal colon cells (CCD-18Co), in vivo and in human colon organoids. The in vivo acute oral toxicity data showed that the LD50 values are greater than 2000 mg/kg for both the SiO2 and TiO2 nanoparticles; however, the SiO2 and TiO2 nanoparticles induced cytotoxicity in two-dimensional CCD-18Co cells and three-dimensional CCD-18Co spheroids and human colon organoids, with IC50 values of 0.6, 0.8 and 0.3 mM for SiO2 and 2.5, 1.1 and 12.5 mM for TiO2 nanoparticles, respectively. The data suggest that, when SiO2 and TiO2 are in nanoparticle form, cytotoxicity is induced; thus, care should be taken with these materials.
Kinetics of the Lattice Response to Hydrogen Absorption in Thin Pd and CoPd Films
Authors: Das Sudhansu Sekhar , Kopnov Gregory , Gerber Alexander
Published: August 2020

Hydrogen can penetrate reversibly a number of metals, occupy the interstitial sites and cause large expansion of the crystal lattice. The question discussed here is whether the kinetics of the structural response matches hydrogen absorption. We show that thin Pd and CoPd films exposed to a relatively rich hydrogen atmosphere (4% H2) inflate irreversibly, demonstrate the controllable shape memory, and duration of the process can be of orders of magnitude longer than hydrogen absorption. The dynamics of the out-of-equilibrium plastic creep are well described by the Avrami-type model of the nucleation and lateral domain wall expansion of the swelled sites.
Synthesis and Initial Evaluation of a Novel Fluorophore for Selective FMDV 3C Protease Detection
Authors: Malik Samerah , Sinclair Alex , Ryan Ali , Le Gresley Adam
Published: August 2020

The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.
Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease
Authors: Lee Joo-Eun , Sim Hyuna , Yoo Hee Min , Lee Minhyung , Baek Aruem , Jeon Young-Joo , Seo Kang-Sik , Son Mi-Young , Yoon Joo Seog , Kim Janghwan
Published: August 2020

Parkinson’s disease (PD) is a well-known age-related neurodegenerative disease. Considering the vital importance of disease modeling based on reprogramming technology, we adopted direct reprogramming to human-induced neuronal progenitor cells (hiNPCs) for in vitro assessment of potential therapeutics. In this study, we investigated the neuroprotective effects of cryptotanshinone (CTN), which has been reported to have antioxidant properties, through PD patient-derived hiNPCs (PD-iNPCs) model with induced oxidative stress and cell death by the proteasome inhibitor MG132. A cytotoxicity assay showed that CTN possesses anti-apoptotic properties in PD-hiNPCs. CTN treatment significantly reduced cellular apoptosis through mitochondrial restoration, such as the reduction in mitochondrial reactive oxygen species and increments of mitochondrial membrane potential. These effects of CTN are mediated via the nuclear factor erythroid 2-related factor 2 (NRF2) pathway in PD-hiNPCs. Consequently, CTN could be a potential antioxidant reagent for preventing disease-related pathological phenotypes of PD.
The Cyclic Nitronate Route to Pharmaceutical Molecules: Synthesis of GSK’s Potent PDE4 Inhibitor as a Case Study
Authors: Pospelov Evgeny V. , Golovanov Ivan S. , Ioffe Sema L. , Sukhorukov Alexey Yu.
Published: August 2020

An efficient asymmetric synthesis of GlaxoSmithKline’s potent PDE4 inhibitor was accomplished in eight steps from a catechol-derived nitroalkene. The key intermediate (3-acyloxymethyl-substituted 1,2-oxazine) was prepared in a straightforward manner by tandem acylation/(3,3)-sigmatropic rearrangement of the corresponding 1,2-oxazine-N-oxide. The latter was assembled by a (4 + 2)-cycloaddition between the suitably substituted nitroalkene and vinyl ether. Facile acetal epimerization at the C-6 position in 1,2-oxazine ring was observed in the course of reduction with NaBH3CN in AcOH. Density functional theory (DFT) calculations suggest that the epimerization may proceed through an unusual tricyclic oxazolo(1,2)oxazinium cation formed via double anchimeric assistance from a distant acyloxy group and the nitrogen atom of the 1,2-oxazine ring.
Anthocyanins Isolated from Vitis coignetiae Pulliat Enhances Cisplatin Sensitivity in MCF-7 Human Breast Cancer Cells through Inhibition of Akt and NF-κB Activation
Authors: Paramanantham Anjugam , Kim Min Jeong , Jung Eun Joo , Kim Hye Jung , Chang Seong-Hwan , Jung Jin-Myung , Hong Soon Chan , Shin Sung Chul , Kim Gon Sup , Lee Won Sup
Published: August 2020

Anthocyanins isolated from Vitis coignetiae Pulliat (Meoru in Korea) (AIMs) have various anti-cancer properties by inhibiting Akt and NF-κB which are involved in drug resistance. Cisplatin (CDDP) is one of the popular anti-cancer agents. Studies reported that MCF-7 human breast cancer cells have high resistance to CDDP compared to other breast cancer cell lines. In this study, we confirmed CDDP resistance of MCF-7 cells and tested whether AIMs can overcome CDDP resistance of MCF-7 cells. Cell viability assay revealed that MCF-7 cells were more resistant to CDDP treatment than MDA-MB-231 breast cancer cells exhibiting aggressive and high cancer stem cell phenotype. AIMs significantly augmented the efficacy of CDDP with synergistic effects on MCF-7 cells. Molecularly, Western blot analysis revealed that CDDP strongly increased Akt and moderately reduced p-NF-κB and p-IκB and that AIMs inhibited CDDP-induced Akt activation, and augmented CDDP-induced reduction of p-NF-κB and p-IκB in MCF-7 cells. In addition, AIMs significantly downregulated an anti-apoptotic protein, XIAP, and augmented PARP-1 cleavage in CDDP-treated MCF-7 cells. Moreover, under TNF-α treatment, AIMs augmented CDDP efficacy with inhibition of NF-κB activation on MCF-7 cells. In conclusion, AIMs enhanced CDDP sensitivity by inhibiting Akt and NF-κB activity of MCF-7 cells that show relative intrinsic CDDP resistance.
The Role of β-Cyclodextrin in the Textile Industry—Review
Authors: Bezerra Fabricio Maestá , Lis Manuel José , Firmino Helen Beraldo , Dias da Silva Joyce Gabriella , Curto Valle Rita de Cassia Siqueira , Borges Valle José Alexandre , Scacchetti Fabio Alexandre Pereira , Tessaro André Luiz
Published: August 2020

β-Cyclodextrin (β-CD) is an oligosaccharide composed of seven units of D-(+)-glucopyranose joined by α-1,4 bonds, which is obtained from starch. Its singular trunk conical shape organization, with a well-defined cavity, provides an adequate environment for several types of molecules to be included. Complexation changes the properties of the guest molecules and can increase their stability and bioavailability, protecting against degradation, and reducing their volatility. Thanks to its versatility, biocompatibility, and biodegradability, β-CD is widespread in many research and industrial applications. In this review, we summarize the role of β-CD and its derivatives in the textile industry. First, we present some general physicochemical characteristics, followed by its application in the areas of dyeing, finishing, and wastewater treatment. The review covers the role of β-CD as an auxiliary agent in dyeing, and as a matrix for dye adsorption until chemical modifications are applied as a finishing agent. Finally, new perspectives about its use in textiles, such as in smart materials for microbial control, are presented.