bluuebunny/crossref_metadata_2025 huggingface dataset:
Computer-Assisted Surgery Enables Beginner Surgeons, Under Expert Guidance, to Achieve Long-Term Clinical Results not Inferior to Those of a Skilled Surgeon in Knee Arthroplasty
Authors: Larrainzar-Garijo Ricardo , Molanes-López Elisa M. , Cañones-Martín Miguel , Murillo-Vizuete David , Valencia-Santos Natalia , Garcia-Bogalo Raul , Corella-Montoya Fernando
Published: June 2022

Purpose

The purpose of this study is to determine whether the use of a surgical navigation system in total knee replacement (TKR) enables beginner and intermediate surgeons to achieve clinical PROM outcomes as good as those conducted by expert surgeons in the long term.

Methods

We enrolled 100 consecutive patients whose total navigated knee arthroplasty (TKA) was performed in our institution from 2008 to 2010. According to the principal surgeon's surgical experience, the patients were divided into three groups: (1) beginner surgeons, with no more than 30 previous knee replacement performances, (2) intermediate surgeons, with more than 30 but not more than 300, and (3) expert surgeons, with more than 300 knee replacements. Demographic data collected on the cohort included gender, laterality, age, and body mass index (BMI). The outcome measures assessed were Forgotten Joint Score (FJS), implant positioning, limb alignment, and prosthesis survival rate. A margin of equivalence of ± 18.5 points in the FJS scale was prespecified in terms of the minimal clinically important difference (MCID) to compare the FJS results obtained in the long period between the groups of interest.

Results

The mean follow-up was 11.10 ± 0.78, 10.86 ± 0.66, and 11.30 ± 0.74 years, respectively, for each of the groups. The long-term FJS mean score was 80.86 ± 21.88, 81.36 ± 23.87, and 90.48 ± 14.65 for each group. The statistical analysis proved noninferiority and equivalence in terms of the FJS results reported in the long term by patients in Groups 1 or 2 compared to those in Group 3. More specifically, it has been proved that the mean difference between groups is within the interval of equivalence defined in terms of the MCID. The overall prostheses survival rate was 93.7%.

Conclusion

Navigated assisted TKA, under expert guidance, can be as effective when performed by beginner or intermediate surgeons as performed by senior surgeons regarding the accuracy of implant positioning, limb alignment, and long-term clinical outcome.
Replies to critics
Authors: Rosenkranz Sven
Published: June 2022

Responding to the critical comments by the symposiasts Julien Dutant, Niccolò Rossi, Martin Smith, Daniel Waxman, and Yiwen Zhan, I further elaborate, refine, and defend the account of epistemic justification I advanced in my book Justification as Ignorance (Oxford: Oxford University Press, 2021). Central issues tackled here include logical omniscience and non-normal epistemic logics, the luminosity of epistemic justification, methods for telling what one is in no position to know, and the relation between doxastic and propositional justification.
The Decolonization of Knowledge
Authors: Jansen Jonathan D. , Walters Cyrill A.
Published: June 2022

In 2015, students at the University of Cape Town used the slogan #RhodesMustFall to demand that a monument of Cecil John Rhodes, the empire builder of British South Africa, be removed from the university campus. Soon students at Oxford University called for the removal of a statue of Rhodes from Oriel College. The radical idea of decolonization at the forefront of these student protests continues to be a key element in South African educational institutions as well as those in Europe and North America. This book explores the uptake of decolonization in the institutional curriculum, given the political demands for decolonization on South African campuses, and the generally positive reception of the idea by university leaders. Based on interviews with more than two hundred academic teachers at ten universities, this is an innovative account of how institutions have engaged with, subverted, and transformed the decolonization movement since #RhodesMustFall.
Petticoat Power? Mary Astell's Appropriation of Heroic Virtue for Women
Authors: BROWN DEBORAH J. , BROAD JACQUELINE
Published: June 2022

Several recent studies devote themselves to Mary Astell's feminist theory of virtue—her ‘serious proposal to the ladies’ to help women obtain wisdom, equality, and happiness, despite the prejudices of seventeenth-century custom. But there has been little scholarship on Astell's conception of heroic virtues, those exceptional character traits that raise their bearers above the ordinary course of nature. Astell's appropriation of heroic virtue poses a number of philosophical difficulties for her feminist ethics—heroic virtues are characteristically masculine, exceptional, and individualistic, ill-suited to a community-oriented feminism aimed at ordinary women. In this paper, we seek to investigate—and then dispel—these key difficulties. Our intention is to generate a new understanding of Astell's theory of virtue as a unique and sophisticated theory that equalizes and naturalizes heroic virtue for women.
Rising from the pandemic ashes: Reflections on burnout and resiliency from the infection prevention and antimicrobial stewardship workforce
Authors: Nori Priya , Stevens Michael P. , Patel Payal K.
Published: June 2022

Hospital epidemiologists, infection preventionists, and antimicrobial stewards are integral to the pandemic workforce. However, regardless of pandemic surge or postsurge conditions, their workload remains high due to constant vigilance for new variants, emerging data, and evolving public health guidance. We describe the factors that have led to burnout and suggest strategies to enhance resilience.
Pandemic crisis and employee skills: how emotion regulation and improvisation limit the damaging effects of perceived pandemic threats on job performance
Authors: De Clercq Dirk , Fatima Tasneem , Jahanzeb Sadia
Published: June 2022

This study details the possible escalation of employees' perceptions of pandemic threats into diminished job performance, while considering a mediating role of their sense of job insecurity and a moderating role of their emotion regulation and improvisation skills. Results based on multisource, three-wave data show that employees' perceived pandemic threats compromise their work-related performance, because they believe that their job is at risk. This mediating role of perceived job insecurity is mitigated by employees' greater ability to control their emotions and come up with novel ideas on the spot. This study accordingly pinpoints employees' conviction that they may not be able to keep their jobs as a crucial mechanism by which the hardships of a global pandemic generate negative performance consequences, as well as how organizations can contain this risk by helping employees hone their pertinent personal skills.
Winning strategies: the emergence of base 2 in the game of nim
Authors: Friedman Eric J. , Landsberg Adam S.
Published: June 2022

Many players know that the secret of winning the game of nim (and other “impartial” combinatorial games) is to write the sizes of the game’s piles in base 2 and then add them together without carry. The proof of this well-known procedure (described below) is both straightforward and convincing. Nonetheless, the procedure still appears magical, as though a rabbit has been pulled out of a hat. Astute students (and frustrated professors) often ask why the winning strategy for such games involves base 2, and not some other base. After all, nothing about the game of nim itself – the game rules, the configuration of the tokens, etc. – provides any hints about the origin of base 2 in this setting. Minimal insight is offered by most published proofs, which themselves tend to either appear almost wizardly in nature (i.e. assume the base-2 method and show that it miraculously solves the problem) or employ combinatorial arguments that supply little abstract intuition (at least to the authors of this article).
Fibonacci-Lucas hyperbolas
Authors: Sporn Howard
Published: June 2022

Let us define a Fibonacci-Lucas hyperbola as a hyperbola passing through an infinite number of points of the form (Fm, Ln), where the Fm are distinct Fibonacci numbers (0, 1, 1, 2, 3, 5, 8, 13, 21,…, where F0 = 0), and the Ln are distinct Lucas numbers (2, 1, 3, 4, 7, 11, 18, 29,…,. where L0 = 2). The simplest examples are 5x2 - y2 = 4, which contains the points (Fk, Lk) with odd subscripts, e.g. (1, 1), (2, 4), (5, 11), and 5x2 - y2 = -4, which contains the points with even subscripts, e.g. (0, 2), (1, 3), (3, 7); (see [1, 2]). These follow immediately from the identity
(1)
$$L_n^2 - 5F_n^2 = 4{( - 1)^n}.$$

Our goal is to find more of these Fibonacci-Lucas hyperbolas.
Dissecting attached squares
Authors: MacHale Des
Published: June 2022

Many papers and indeed books have been written about the problem of dissecting a number of squares of different integer side length and reassembling the pieces to form a single square (see [1], [2] and [3]). For example, in the case of 32 + 42 = 52, we can achieve a four-piece dissection as follows:
Mediators and moderators in the relationship between maternal childhood adversity and children's emotional and behavioural development: a systematic review and meta-analysis
Authors: Ma Xuemei , Biaggi Alessandra , Sacchi Chiara , Lawrence Andrew J. , Chen Pei-Jung , Pollard Rebecca , Matter Maryam , Mackes Nuria , Hazelgrove Katie , Morgan Craig , Harding Seeromanie , Simonelli Alessandra , Schumann Gunter , Pariante Carmine M. , Mehta Mitul , Montana Giovanni , Rodriguez-Mateos Ana , Nosarti Chiara , Dazzan Paola
Published: June 2022

Maternal experiences of childhood adversity can increase the risk of emotional and behavioural problems in their children. This systematic review and meta-analysis provide the first narrative and quantitative synthesis of the mediators and moderators involved in the link between maternal childhood adversity and children's emotional and behavioural development. We searched EMBASE, PsycINFO, Medline, Cochrane Library, grey literature and reference lists. Studies published up to February 2021 were included if they explored mediators or moderators between maternal childhood adversity and their children's emotional and behavioural development. Data were synthesised narratively and quantitatively by meta-analytic approaches. The search yielded 781 articles, with 74 full-text articles reviewed, and 41 studies meeting inclusion criteria. Maternal mental health was a significant individual-level mediator, while child traumatic experiences and insecure maternal–child attachment were consistent family-level mediators. However, the evidence for community-level mediators was limited. A meta-analysis of nine single-mediating analyses from five studies indicated three mediating pathways: maternal depression, negative parenting practices and maternal insecure attachment, with pooled indirect standardised effects of 0.10 [95% CI (0.03–0.17)), 0.01 (95% CI (−0.02 to 0.04)] and 0.07 [95% CI (0.01–0.12)], respectively. Research studies on moderators were few and identified some individual-level factors, such as child sex (e.g. the mediating role of parenting practices being only significant in girls), biological factors (e.g. maternal cortisol level) and genetic factors (e.g. child's serotonin-transporter genotype). In conclusion, maternal depression and maternal insecure attachment are two established mediating pathways that can explain the link between maternal childhood adversity and their children's emotional and behavioural development and offer opportunities for intervention.
Using social networking sites as a language teaching and learning environment
Authors: Barrot Jessie S.
Published: June 2022

The growing adoption of emerging technologies for language pedagogy, literacy development, and language assessment has accelerated computer-assisted language learning (CALL) as a major field of education and led to the establishment of major specialized journals, such as Language Learning and Technology, Computer Assisted Language Learning, and ReCALL. As technologies further advanced, we began to see individuals and schools increasingly adopt CALL technologies and use their interactive features to facilitate language learning. Consequently, CALL research began to gain momentum and expand its research foci during the mid-1990s (Levy, 2000; Uzunboylu & Ozcinar, 2009). This expansion gave birth to some independent and stand-alone subfields, such as computer-assisted language testing (CALT) (Parmaxi et al., 2013).
Comparing the IVM laboratory outcomes between stimulated IVF with unstimulated natural cycles
Authors: Khalili Mohammad Ali , Mohsenzadeh Mehdi , Karimi Zarchi Mojgan , Vatanparast Mahboubeh
Published: June 2022

Recently, more attention has been raised towards fertility preservation in women with cancer. One option is in vitro maturation (IVM) of the immature oocytes as there is not enough time for induction of an ovarian stimulation protocol. The aim was to compare the IVM laboratory outcomes between stimulated and unstimulated (natural) in vitro fertilization (IVF) cycles. In total, 234 immature oocytes collected from 15 cancer patients who underwent an IVM programme (natural IVM) and 23 IVF cycles with a controlled ovarian hyperstimulation protocol (stimulated IVM) were analyzed. The oocyte morphology, zona pellucida (ZP), and meiotic spindle presence were measured using PolScope technology. Also, the rates of oocyte maturation and fertilization were assessed in both groups. The IVM rate was higher in the stimulated cycle (P < 0.05), but the fertilization rate was insignificant in comparison with unstimulated cycles. There were no significant differences in the spindle visualization and ZP birefringence scoring between the groups (P > 0.05). The oocyte normal morphology was better in the stimulated cycle compared with the natural cycle (P < 0.05). In conclusion, IVM can be recommended for cancer patients as an alternative treatment when there is insufficient time for conventional IVF before chemotherapy initiation.
Morphological description of gametes in cave and surface populations of Astyanax mexicanus (De Filippi, 1853)
Authors: Rodríguez-Ballesteros Víctor , Mendoza-Garfias Berenit , Ulloa-Arvizu Raúl , Balcazar Alberto , Ornelas-García C. Patricia
Published: June 2022

The Mexican tetra Astyanax mexicanus presents two contrasting morphs, a widely distributed surface morph and a cave-adapted morph. These cave-adapted morphs have evolved independently from two different lineages (i.e. ‘old’ and ‘new’ lineages); therefore, this model system gives a unique opportunity to explore parallel adaptive evolution in biological traits. The present study corresponds to the first morphological description of the Astyanax mexicanus maturation process of the spermatozoa and oocytes, using thermal and hormonal stimuli to promote spermatogenesis and oogenesis, considering surface and cave morphs from both lineages. We corroborate the relevance of thermal and hormonal stimuli to promote gamete maturation. The hormone Ovaprim (GnRHa + Domperidone) is an effective promoter of ovarian development, maturation end in oocytes and spawning in Astyanax mexicanus. The sperm morphology of Astyanax mexicanus includes the sperm head, the midpiece, and tail or flagellum. We found differences in the spermatozoan total length between environments (F = 9.929, P = 0.05) and linages (F = 49.86, P = 0.005). The oocytes showed a spherical conformation with a mean diameter of 822.4 ± 194.1 μm for the surface populations, and 604.6 ± 38.3 µm for the cave populations. The oocyte chorion presents ridges and grooves that are arranged radially towards the micropyle. A plug in the micropyle zone was observed after fertilization, confirmed by the outer membrane of the chorion, which provides some weak adhesiveness to the substrate. We observed differences in chorion thickness between the contrasting environmental conditions. This is the first morphological characterization of the Sótanos Vázquez, Escondido and Tigre, which previous to this study were only known from speleological expeditions, with no previous biological information available.
Can mesenchymal stem cells derived from adipose tissue and their conditioned medium improve ovarian functions? A mini-review
Authors: Pouryousefi-koodehi Tayebe , Shayegan Shika , Hashemi Soheyla , Arefnezhad Reza , Roghani-Shahraki Hossein , Motedayyen Hossein , Taghizabet Neda , Rezaei-Tazangi Fatemeh
Published: June 2022

Stable ovarian function is a key factor in the performance of the reproductive system. In contrast, some ovarian function-related diseases, such as polycystic ovarian syndrome, premature ovarian failure (POF), and ovarian cancer, are the main cause of infertility and death of women around the world. Despite multiple attempts, there are no effective tools against these conditions; however, mesenchymal stem cell-based therapy, especially using adipose tissue, has attracted much attention in medicine in light of its advantages such as easy isolation and accessibility. Conversely, it has been suggested that MSC-conditioned medium (CM) can restore injured tissues and has high immunocompatibility. So, here, we will summarize the effects of administration of MSCs and CM derived from adipose tissue on ovarian functions and related diseases.
Evaluation of the impact and acceptability of Cognitive Behavioural Analysis System of Psychotherapy (CBASP) for chronic depression
Authors: Linstead Jonathan , Doyle Michael
Published: June 2022

Background:

Cognitive Behavioural Analysis System of Psychotherapy (CBASP) is an evidenced based treatment model for chronically depressed patients.

Aims:

The main aim of this service evaluation was to assess the acceptability and clinical impact of CBASP for chronic depression within an Improving Access to Psychological Therapies (IAPT) service.

Method:

Routinely collected data were analysed for all patients that received CBASP treatment focussing on the recovery rates of these patients in terms of depression, anxiety and social functioning. Interviews were conducted with patients who had recently been discharged from CBASP therapist within one month of the follow-up date, explore their experiences of therapy.

Results:

Outcome data for 27 patients suggested substantial reduction in scoring on measures of depression and anxiety following CBASP treatment. Across all interviews it was clear that patients developed an insight and understanding of how their behaviours affect the outcome of interpersonal situations.

Conclusions:

Results from this service evaluation suggest that CBASP is acceptable to service users and has a positive clinical impact in terms of IAPT recovery targets for anxiety, depression and social functioning.
On the Rayleigh Wave Induced Ionospheric Perturbations During the Mw 9.0 11 March 2011 Tohoku‐Oki Earthquake
Authors: Thomas Dhanya , Bagiya Mala S. , Hazarika Nava Kumar , Ramesh D. S.
Published: June 2022

Spatial and temporal characteristics of Rayleigh wave generated ionospheric perturbations are well documented based on the ionospheric measurements using Global Positioning System (GPS)‐Total Electron Content (TEC) technique. However, due to integrated nature of the GPS recorded TEC, the actual detection altitudes of these perturbations could not be determined. In general, the maximum electron density altitude (hmF2) is assumed as the perturbation detection altitude in GPS‐TEC. To validate this assumption, case studies focusing on the early detection of ionospheric perturbations induced by direct epicentral waves during the Mw 7.4 9 March 2011 Sanriku‐Oki and Mw 9.0 11 March 2011 Tohoku‐Oki earthquakes were performed. In the present study, we attempt to estimate the detection altitudes of GPS‐TEC derived ionospheric perturbations generated by the propagating Rayleigh surface waves following the Mw 9.0 11 March 2011 Tohoku‐Oki earthquake. Based on the 3D ray tracing of Rayleigh wave generated acoustic waves in the atmosphere and realistic GPS station‐satellite line of sight geometry, we could efficaciously compute the actual detection altitudes of Rayleigh wave generated coseismic ionospheric perturbations (CIPs). Further, our study also demonstrates the possible detection of CIPs progressively at different altitudes based on varying satellite geometries.
Unraveling the Roles of Saltation Bombardment and Atmospheric Instability on Magnitude and Size Distribution of Dust Emission Fluxes: Lessons From the JADE and WIND‐O‐V Experiments
Authors: Alfaro S. C. , Bouet C. , Khalfallah B. , Shao Y. , Ishizuka M. , Labiadh M. , Marticorena B. , Laurent B. , Rajot J. L.
Published: June 2022

The size distribution of the vertical flux of dust freshly emitted from a wind‐eroded surface was recently shown to depend on the thermal stratification of the surface boundary layer (SBL). These new results question the way dust emission is currently represented in the dust models and emphasize the need to identify the factors controlling the intensity and size‐resolved dust flux at emission. In this study, we re‐analyze the data of two major campaigns (JADE and WIND‐O‐V) performed on unvegetated plots and during which the characteristics of the (a) surface of the eroding fields, (b) aerodynamic conditions (wind speed, stability of the SBL), (c) saltation flux (intensity and size distribution), and (d) vertical dust flux (intensity and size distribution) determined by the gradient method were carefully documented. The magnitude and size distribution of the vertical dust flux are found to be deeply intertwined and to be controlled in the first place by the kinetic energy of the saltating sand grains, and to a lesser extent by the size‐dependent uplift of the sandblasted particles. In unstable conditions coarser sand grains are mobilized, which increases the kinetic energy of the saltation flux and leads to the production of finer particles by sandblasting. Conversely, the uplift of supermicron particles is facilitated by the increase of the wind friction velocity, which results in an enrichment of the vertical dust flux in the coarsest particles at large wind speeds. The implications of these new findings are particularly important for the modeling of the dust emission/transport/deposition cycle.
From Lake to River: Documenting an Environmental Transition Across the Jura/Knockfarril Hill Members Boundary in the Glen Torridon Region of Gale Crater (Mars)
Authors: Caravaca Gwénaël , Mangold Nicolas , Dehouck Erwin , Schieber Juergen , Zaugg Louis , Bryk Alexander B. , Fedo Christopher M. , Le Mouélic Stéphane , Le Deit Laetitia , Banham Steven G. , Gupta Sanjeev , Cousin Agnès , Rapin William , Gasnault Olivier , Rivera‐Hernández Frances , Wiens Roger C. , Lanza Nina L.
Published: June 2022

Between January 2019 and January 2021, the Mars Science Laboratory team explored the Glen Torridon (GT) region in Gale crater (Mars), known for its orbital detection of clay minerals. Mastcam, Mars Hand Lens Imager, and ChemCam data are used in an integrated sedimentological and geochemical study to characterize the Jura member of the upper Murray formation and the Knockfarril Hill member of the overlying Carolyn Shoemaker formation in northern GT. The studied strata show a progressive transition represented by interfingering beds of fine‐grained, recessive mudstones of the Jura member and coarser‐grained, cross‐stratified sandstones attributed to the Knockfarril Hill member. Whereas the former are interpreted as lacustrine deposits, the latter are interpreted as predominantly fluvial deposits. The geochemical composition seen by the ChemCam instrument show K2O‐rich mudstones (∼1–2 wt.%) versus MgO‐rich sandstones (>6 wt.%), relative to the average composition of the underlying Murray formation. We document consistent sedimentary and geochemical data sets showing that low‐energy mudstones of the Jura member are associated with the K‐rich endmember, and that high‐energy cross‐stratified sandstones of the Knockfarril Hill member are associated with the Mg‐rich endmember, regardless of stratigraphic position. The Jura to Knockfarril Hill transition therefore marks a significant paleoenvironmental change, where a long‐lived and comparatively quiescent lacustrine setting progressively changes into a more energetic fluvial setting, as a consequence of shoreline regression due to either increased sediment supply or lake‐level drop.
The evolution of BDNF is defined by strict purifying selection and prodomain spatial coevolution, but what does it mean for human brain disease?
Authors: Lucaci Alexander G. , Notaras Michael J. , Kosakovsky Pond Sergei L. , Colak Dilek
Published: June 2022

Brain-Derived Neurotrophic Factor (BDNF) is an essential mediator of brain assembly, development, and maturation. BDNF has been implicated in a variety of brain disorders such as neurodevelopmental disorders (e.g., autism spectrum disorder), neuropsychiatric disorders (e.g., anxiety, depression, PTSD, and schizophrenia), and various neurodegenerative disorders (e.g., Parkinson’s, Alzheimer’s, etc.). To better understand the role of BDNF in disease, we sought to define the evolution of BDNF withinMammalia. We conducted sequence alignment and phylogenetic reconstruction of BDNF across a diverse selection of >160 mammalian species spanning ~177 million years of evolution. The selective evolutionary change was examined via several independent computational models of codon evolution including FEL (pervasive diversifying selection), MEME (episodic selection), and BGM (structural coevolution of sites within a single molecule). We report strict purifying selection in the main functional domain of BDNF (NGF domain, essentially comprising the mature BDNF protein). Additionally, we discover six sites in our homologous alignment which are under episodic selection in early regulatory regions (i.e. the prodomain) and 23 pairs of coevolving sites that are distributed across the entirety of BDNF. Coevolving BDNF sites exhibited complex spatial relationships and geometric features including triangular relations, acyclic graph networks, double-linked sites, and triple-linked sites, although the most notable pattern to emerge was that changes in the mature region of BDNF tended to coevolve along with sites in the prodomain. Thus, we propose that the discovery of both local and distal sites of coevolution likely reflects ‘evolutionary fine-tuning’ of BDNF’s underlying regulation and function in mammals. This tracks with the observation that BDNF’s mature domain (which encodes mature BDNF protein) is largely conserved, while the prodomain (which is linked to regulation and its own unique functionality) exhibits more pervasive and diversifying evolutionary selection. That said, the fact that negative purifying selection also occurs in BDNF’s prodomain also highlights that this region also contains critical sites of sensitivity which also partially explains its disease relevance (via Val66Met and other prodomain variants). Taken together, these computational evolutionary analyses provide important context as to the origins and sensitivity of genetic changes within BDNF that may help to deconvolute the role of BDNF polymorphisms in human brain disorders.
ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination
Authors: Geng Shengjun , Peng Wen , Wang Xue , Hu Xiaosong , Liang Hanghua , Hou Jianbing , Wang Feng , Zhao Gaichao , Lü Muhan , Cui Hongjuan
Published: June 2022

Ariadne homolog 2 (ARIH2) is a key member of the RING-between-RING (RBR) E3 ligase family, which is characterized by an RBR domain involved in the polyubiquitination process. However, the molecular mechanism and biological function of ARIH2 in the pathogenesis of gastric cancer remain unclear. In this paper, we found that high ARIH2 expression is correlated with poor prognosis in gastric cancer patients and that ARIH2 can significantly promote the proliferation of gastric cancer cells. The effect of ARIH2 knockdown on colony formation and tumorigenesis of gastric cancer cells was also shown both in vivo and in vitro. Further mechanistic investigations revealed that ARIH2 interacts with p21 and induces p21 ubiquitination, and that the K48 residue of ubiquitin and the K161 residue of p21 play key roles in ARIH2-mediated p21 ubiquitination. We identified ARIH2 as an E3 ligase of p21 by an in vitro ubiquitination assay. In addition, ARIH2 knockdown induced DNA damage, and then induced cell apoptosis and regulated the chemosensitivity of gastric cancer cells after combined treatment with 5-fluorouracil. Generally, our results indicated that ARIH2 promotes the proliferation of gastric cancer cells and regulates p21 expression. These data demonstrate the need to further evaluate the potential therapeutic implications of ARIH2 in gastric cancer.
Host control and the evolution of cooperation in host microbiomes
Authors: Sharp Connor , Foster Kevin R.
Published: June 2022

Humans, and many other species, are host to diverse symbionts. It is often suggested that the mutual benefits of host-microbe relationships can alone explain cooperative evolution. Here, we evaluate this hypothesis with evolutionary modelling. Our model predicts that mutual benefits are insufficient to drive cooperation in systems like the human microbiome, because of competition between symbionts. However, cooperation can emerge if hosts can exert control over symbionts, so long as there are constraints that limit symbiont counter evolution. We test our model with genomic data of two bacterial traits monitored by animal immune systems. In both cases, bacteria have evolved as predicted under host control, tending to lose flagella and maintain butyrate production when host-associated. Moreover, an analysis of bacteria that retain flagella supports the evolution of host control, via toll-like receptor 5, which limits symbiont counter evolution. Our work puts host control mechanisms, including the immune system, at the centre of microbiome evolution.
Trapping and detecting nanoplastics by MXene-derived oxide microrobots
Authors: Urso Mario , Ussia Martina , Novotný Filip , Pumera Martin
Published: June 2022

Nanoplastic pollution, the final product of plastic waste fragmentation in the environment, represents an increasing concern for the scientific community due to the easier diffusion and higher hazard associated with their small sizes. Therefore, there is a pressing demand for effective strategies to quantify and remove nanoplastics in wastewater. This work presents the “on-the-fly” capture of nanoplastics in the three-dimensional (3D) space by multifunctional MXene-derived oxide microrobots and their further detection. A thermal annealing process is used to convert Ti3C2Tx MXene into photocatalytic multi-layered TiO2, followed by the deposition of a Pt layer and the decoration with magnetic γ-Fe2O3 nanoparticles. The MXene-derived γ-Fe2O3/Pt/TiO2 microrobots show negative photogravitaxis, resulting in a powerful fuel-free motion with six degrees of freedom under light irradiation. Owing to the unique combination of self-propulsion and programmable Zeta potential, the microrobots can quickly attract and trap nanoplastics on their surface, including the slits between multi-layer stacks, allowing their magnetic collection. Utilized as self-motile preconcentration platforms, they enable nanoplastics’ electrochemical detection using low-cost and portable electrodes. This proof-of-concept study paves the way toward the “on-site” screening of nanoplastics in water and its successive remediation.
RNase III-CLASH of multi-drug resistant Staphylococcus aureus reveals a regulatory mRNA 3′UTR required for intermediate vancomycin resistance
Authors: Mediati Daniel G. , Wong Julia L. , Gao Wei , McKellar Stuart , Pang Chi Nam Ignatius , Wu Sylvania , Wu Winton , Sy Brandon , Monk Ian R. , Biazik Joanna M. , Wilkins Marc R. , Howden Benjamin P. , Stinear Timothy P. , Granneman Sander , Tree Jai J.
Published: June 2022

Treatment of methicillin-resistant Staphylococcus aureus infections is dependent on the efficacy of last-line antibiotics including vancomycin. Treatment failure is commonly linked to isolates with intermediate vancomycin resistance (termed VISA). These isolates have accumulated point mutations that collectively reduce vancomycin sensitivity, often by thickening the cell wall. Changes in regulatory small RNA expression have been correlated with antibiotic stress in VISA isolates however the functions of most RNA regulators is unknown. Here we capture RNA–RNA interactions associated with RNase III using CLASH. RNase III-CLASH uncovers hundreds of novel RNA–RNA interactions in vivo allowing functional characterisation of many sRNAs for the first time. Surprisingly, many mRNA–mRNA interactions are recovered and we find that an mRNA encoding a long 3′ untranslated region (UTR) (termed vigR 3′UTR) functions as a regulatory ‘hub’ within the RNA–RNA interaction network. We demonstrate that the vigR 3′UTR promotes expression of folD and the cell wall lytic transglycosylase isaA through direct mRNA–mRNA base-pairing. Deletion of the vigR 3′UTR re-sensitised VISA to glycopeptide treatment and both isaA and vigR 3′UTR deletions impact cell wall thickness. Our results demonstrate the utility of RNase III-CLASH and indicate that S. aureus uses mRNA-mRNA interactions to co-ordinate gene expression more widely than previously appreciated.
Study on the heterogeneity of China’s agricultural economic growth in the context of temperature shocks
Authors: Yuan Bin , Cui Yuhu , Wang Xueye , Xu Hanxiao
Published: June 2022

Under the background of the new development concept, compared with the absolute impacts, the relative impacts of climate change on agricultural growth deserve more attention. Based on the data from China for years 1991 and 2018, this paper uses historical fluctuations in temperature within cities to identify the heterogeneous effects on aggregate agricultural outcomes during farming and fallow periods. The results show that: first, as temperature rises reduce the economic growth rate of each agricultural sector, and the areas that are relatively vulnerable (i.e., areas where disposable income of farm households is below the sample mean) are more significantly affected by the negative impact of temperature rise; second, the impact of temperature rise on agricultural economic growth is mainly concentrated in the farming period, while the marginal damage of temperature rise is on a decreasing trend; third, the heterogeneous impact of temperature rise on agricultural economic growth during the agricultural fallow period is also not negligible. At the same time, its impact on agricultural economy is still in the primary stage, that is, its marginal damage tends to increase with the increase in temperature fluctuation. These results inform identifying the climate’s role in agricultural development and provide a theoretical and operational perspective for further optimizing the adaptive policy systems. With wide coverage of adaptive technology, we should pay more attention to the even distribution of technological dividends and continuously improve the coping ability of vulnerable groups.
Incongruences between morphology and molecular phylogeny provide an insight into the diversification of the Crocidura poensis species complex
Authors: Voet Inessa , Denys Christiane , Colyn Marc , Lalis Aude , Konečný Adam , Delapré Arnaud , Nicolas Violaine , Cornette Raphaël
Published: June 2022

Untangling the factors of morphological evolution has long held a central role in the study of evolutionary biology. Extant speciose clades that have only recently diverged are ideal study subjects, as they allow the examination of rapid morphological variation in a phylogenetic context, providing insights into a clade’s evolution. Here, we focus on skull morphological variability in a widely distributed shrew species complex, the Crocidura poensis species complex. The relative effects of taxonomy, size, geography, climate and habitat on skull form were tested, as well as the presence of a phylogenetic signal. Taxonomy was the best predictor of skull size and shape, but surprisingly both size and shape exhibited no significant phylogenetic signal. This paper describes one of the few cases within a mammal clade where morphological evolution does not match the phylogeny. The second strongest predictor for shape variation was size, emphasizing that allometry can represent an easily accessed source of morphological variability within complexes of cryptic species. Taking into account species relatedness, habitat preferences, geographical distribution and differences in skull form, our results lean in favor of a parapatric speciation model within this complex of species, where divergence occurred along an ecological gradient, rather than a geographic barrier.
Modeling synergistic effects by using general Hill-type response surfaces describing drug interactions
Authors: Schindler Michael
Published: June 2022

The classification of effects caused by mixtures of agents as synergistic, antagonistic or additive depends critically on the reference model of ’null interaction’. Two main approaches to describe co-operative effects are currently in use, the Additive Dose (ADM) or concentration addition (CA) and the Multiplicative Survival (MSM) or independent action (IA) models. Recently we proposed an approach which describes ’zero-interaction’ surfaces based on the only requirement that simultaneous administration of different drugs leads to Hill-type response surfaces, which are solutions of the underlying logistic differential equations. No further assumptions, neither on mechanisms of action nor on limitations of parameter combinations are required. This defines—and limits—the application range of our approach. Resting on the same principle, we extend this ansatz in the present paper in order to describe deviations from the reference surface by generalized Hill-type functions. To this end we introduce two types of parameters, perturbations of the pure drug Hill-parameters and interaction parameters that account for n-tuple interactions between all components of a mixture. The resulting ‘full-interaction’ response surface is a valid solution of the basic partial differential equation (PDE), satisfying appropriate boundary conditions. This is true irrespective of its actual functional form, as within our framework the number of parameters is not fixed. We start by fitting the experimental data to the ‘full-interaction’ model with the maximum possible number of parameters. Guided by the fit-statistics, we then gradually remove insignificant parameters until the optimum response surface model is obtained. The ’full-interaction’ Hill response surface ansatz can be applied to mixtures of n compounds with arbitrary Hill parameters including those describing baseline effects. Synergy surfaces, i.e., differences between full- and null-interaction models, are used to identify dose-combinations showing peak synergies. We apply our approach to binary and ternary examples from the literature, which range from mixtures behaving according to the null-interaction model to those showing strong synergistic or antagonistic effects. By comparing ’null-’ and ’full-response’ surfaces we identify those dose-combinations that lead to maximum synergistic or antagonistic effects. In one example we identify both synergistic and antagonistic effects simlutaneously, depending on the dose-ratio of the components. In addition we show that often the number of parameters necessary to describe the response can be reduced without significantly affecting the accuracy. This facilitates an analysis of the synergistic effects by focussing on the main factors causing the deviations from ’null-interaction’.
SIN-3 functions through multi-protein interaction to regulate apoptosis, autophagy, and longevity in Caenorhabditis elegans
Authors: Konwar Chandrika , Maini Jayant , Kohli Surbhi , Brahmachari Vani , Saluja Daman
Published: June 2022

SIN3/HDAC is a multi-protein complex that acts as a regulatory unit and functions as a co-repressor/co-activator and a general transcription factor. SIN3 acts as a scaffold in the complex, binding directly to HDAC1/2 and other proteins and plays crucial roles in regulating apoptosis, differentiation, cell proliferation, development, and cell cycle. However, its exact mechanism of action remains elusive. Using the Caenorhabditis elegans (C. elegans) model, we can surpass the challenges posed by the functional redundancy of SIN3 isoforms. In this regard, we have previously demonstrated the role of SIN-3 in uncoupling autophagy and longevity in C. elegans. In order to understand the mechanism of action of SIN3 in these processes, we carried out a comparative analysis of the SIN3 protein interactome from model organisms of different phyla. We identified conserved, expanded, and contracted gene classes. The C. elegans SIN-3 interactome -revealed the presence of  well-known proteins, such as DAF-16, SIR-2.1, SGK-1, and AKT-1/2, involved in autophagy, apoptosis, and longevity. Overall, our analyses propose  potential mechanisms by which SIN3 participates in multiple biological processes and their conservation across species and identifies candidate genes for further experimental analysis.
Sampling a gradient of red snow algae bloom density reveals novel connections between microbial communities and environmental features
Authors: Tucker Avery E. , Brown Shawn P.
Published: June 2022

Snow algae blooms and associated microbial communities play large roles in snow ecosystem processes. Patterns and mechanisms underpinning snow algae bloom spatial distribution and associated microbial community assembly dynamics are poorly understood. Here we examine associations of microbial communities and environmental measures between/within snow algae blooms. Snows from the Cascade Mountains and the Rocky Mountains (USA) were collected from medial (M), peripheral (P), and adjacent (A) zones of red snow algae blooms. Medial snow shows increased levels of pollen, lower oxidation–reduction potential, decreased algal and increased bacterial richness, and increased levels of potassium when compared to A and P within the same bloom. Between the Cascade and Rocky Mountains, fungal communities are distinct but bacterial and algal communities show little differentiation. A weighted OTU co-expression analysis (WOCNA) explores OTU modules and their differential correlation with environmental features, suggesting certain subcommunities may be altered by ecological patterns. Individual OTU interaction networks (fungi and bacteria) show high levels of connectivity compared to networks based on the red snow algaSanguina nivaloides, which underscores associative differences between algal dominated networks and other taxa.
HDAC6 is a prognostic biomarker that mediates IL-13 expression to regulate macrophage polarization through AP-1 in oral squamous cell carcinoma
Authors: Tseng Chung-Chih , Huang Shi-Ying , Tsai Hung-Pei , Wu Chia-Wei , Hsieh Tsung-Hua
Published: June 2022

Oral squamous cell carcinoma (OSCC) is a common malignant tumor worldwide that is characterized by abnormal lesions or malignant hyperplasia of soft and hard tissues in the oral cavity. Previous research has found that HDAC6 may be a potential therapeutic target for cancer patients and has the ability to regulate immune cells. However, the mechanism of HDAC6 in OSCC pathogenesis is unclear. We collected clinical samples and analyzed the level of HDAC6 in OSCC patients. The results showed that in the high HDAC6 expression group, HDAC6 expression was positively correlated with the grade of OSCC (R = 0.182, P = 0.036) and that this group had a 3.248-fold increase in the mortality risk compared with the low HDAC6 expression group (P = 0.003). Survival analysis also identified a correlation between the expression of HDAC6 and overall survival in OSCC patients, and it was found that the expression of HDAC6 was inversely correlated with survival (P ≤ 0.001). In addition, we found that HDAC6 induced IL-13 expression through AP-1, resulting in M2 polarization of macrophages. Together, these results demonstrate that the level of HDAC6 may be a useful prognostic biomarker and offer a novel immune cell-related therapeutic strategy of targeting IL-13 in OSCC.
Participatory mapping identifies risk areas and environmental predictors of endemic anthrax in rural Africa
Authors: Aminu Olubunmi R. , Forde Taya L. , Ekwem Divine , Johnson Paul , Nelli Luca , Mmbaga Blandina T. , Mshanga Deogratius , Shand Mike , Shirima Gabriel , Walsh Markus , Zadoks Ruth N. , Biek Roman , Lembo Tiziana
Published: June 2022

Disease mapping reveals geographical variability in incidence, which can help to prioritise control efforts. However, in areas where this is most needed, resources to generate the required data are often lacking. Participatory mapping, which makes use of indigenous knowledge, is a potential approach to identify risk areas for endemic diseases in low- and middle-income countries. Here we combine this method with Geographical Information System-based analyses of environmental variables as a novel approach to study endemic anthrax, caused by the spore-forming bacterium Bacillus anthracis, in rural Africa. Our aims were to: (1) identify high-risk anthrax areas using community knowledge; (2) enhance our understanding of the environmental characteristics associated with these areas; and (3) make spatial predictions of anthrax risk. Community members from the Ngorongoro Conservation Area (NCA), northern Tanzania, where anthrax is highly prevalent in both animals and humans, were asked to draw areas they perceived to pose anthrax risks to their livestock on geo-referenced maps. After digitisation, random points were generated within and outside the defined areas to represent high- and low-risk areas, respectively. Regression analyses were used to identify environmental variables that may predict anthrax risk. Results were combined to predict how the probability of being a high-risk area for anthrax varies across space. Participatory mapping identified fourteen discrete high-risk areas ranging from 0.2 to 212.9 km2 in size and occupying 8.4% of the NCA. Areas that pose a high risk of anthrax were positively associated with factors that increase contact with Bacillus anthracis spores rather than those associated with the pathogen’s survival: close proximity to inland water bodies, where wildlife and livestock congregate, and low organic carbon content, which may indicate an increased likelihood of animals grazing close to soil surface and ingesting spores. Predicted high-risk areas were located in the centre of the NCA, which is likely to be encountered by most herds during movements in search for resources. We demonstrate that participatory mapping combined with spatial analyses can provide novel insights into the geography of disease risk. This approach can be used to prioritise areas for control in low-resource settings, especially for diseases with environmental transmission.
Energy harvesting from sonic noises by phononic crystal fibers
Authors: Motaei Farzaneh , Bahrami Ali
Published: June 2022

In this investigation, a phononic crystal-based fiber is proposed for energy harvesting application in metalworking factories. Phononic crystal plays the role of cladding in elastic fiber structure. Each of single-core fibers includes a tungsten hollow cylinder in central region which its internal radius is different in three single-core fibers. Incident waves with central frequency from 25 to 40 kHz of 1/3 octave band are confined in the core region of proposed elastic fibers and transmitted to desired distance. High confinement and transmission ability without significant longitudinal loss make this structure distinct from the other phononic crystals-based energy harvesters. By utilizing of a piezoelectric film at the end of fiber cores, mechanical energy is converted to electrical energy. As proposed elastic fibers confine the applied waves with high quality, the obtained output power is enhanced up to 800 times in comparison with the bare case. Maximum value of extinction ratio between all single core fibers is equal to − 23 dB. Also, longitudinal loss is almost equal to 0.9 dB/km.