bluuebunny/crossref_metadata_2025 huggingface dataset:
The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism
Authors: Musidlak Oskar , Warowicka Alicja , Broniarczyk Justyna , Adamczyk Damian , Goździcka-Józefiak Anna , Nawrot Robert
Published: August 2022

Yellow-orange latex of Chelidonium majus L. has been used in folk medicine as a therapeutic agent against warts and other visible symptoms of human papillomavirus (HPV) infections for centuries. The observed antiviral and antitumor properties of C. majus latex are often attributed to alkaloids contained therein, but recent studies indicate that latex proteins may also play an important role in its pharmacological activities. Therefore, the aim of the study was to investigate the effect of the crude C. majus latex and its protein and alkaloid-rich fractions on different stages of the HPV replication cycle. The results showed that the latex components, such as alkaloids and proteins, decrease HPV infectivity and inhibit the expression of viral oncogenes (E6, E7) on mRNA and protein levels. However, the crude latex and its fractions do not affect the stability of structural proteins in HPV pseudovirions and they do not inhibit the virus from attaching to the cell surface. In addition, the protein fraction causes increased TNFα secretion, which may indicate the induction of an inflammatory response. These findings indicate that the antiviral properties of C. majus latex arise both from alkaloids and proteins contained therein, acting on different stages of the viral replication cycle.
Molecular Events behind the Selectivity and Inactivation Properties of Model NaK-Derived Ion Channels
Authors: Giudici Ana Marcela , Renart María Lourdes , Coutinho Ana , Morales Andrés , González-Ros José Manuel , Poveda José Antonio
Published: August 2022

Y55W mutants of non-selective NaK and partly K+-selective NaK2K channels have been used to explore the conformational dynamics at the pore region of these channels as they interact with either Na+ or K+. A major conclusion is that these channels exhibit a remarkable pore conformational flexibility. Homo-FRET measurements reveal a large change in W55–W55 intersubunit distances, enabling the selectivity filter (SF) to admit different species, thus, favoring poor or no selectivity. Depending on the cation, these channels exhibit wide-open conformations of the SF in Na+, or tight induced-fit conformations in K+, most favored in the four binding sites containing NaK2K channels. Such conformational flexibility seems to arise from an altered pattern of restricting interactions between the SF and the protein scaffold behind it. Additionally, binding experiments provide clues to explain such poor selectivity. Compared to the K+-selective KcsA channel, these channels lack a high affinity K+ binding component and do not collapse in Na+. Thus, they cannot properly select K+ over competing cations, nor reject Na+ by collapsing, as K+-selective channels do. Finally, these channels do not show C-type inactivation, likely because their submillimolar K+ binding affinities prevent an efficient K+ loss from their SF, thus favoring permanently open channel states.
Cartilage Oligomeric Matrix Protein, Diseases, and Therapeutic Opportunities
Authors: Cui Jiarui , Zhang Jiaming
Published: August 2022

Cartilage oligomeric matrix protein (COMP) is an extracellular matrix (ECM) glycoprotein that is critical for collagen assembly and ECM stability. Mutations of COMP cause endoplasmic reticulum stress and chondrocyte apoptosis, resulting in rare skeleton diseases. The bouquet-like structure of COMP allows it to act as a bridging molecule that regulates cellular phenotype and function. COMP is able to interact with many other ECM components and binds directly to a variety of cellular receptors and growth factors. The roles of COMP in other skeleton diseases, such as osteoarthritis, have been implied. As a well-established biochemical marker, COMP indicates cartilage turnover associated with destruction. Recent exciting achievements indicate its involvement in other diseases, such as malignancy, cardiovascular diseases, and tissue fibrosis. Here, we review the basic concepts of COMP and summarize its novel functions in the regulation of signaling events. These findings renew our understanding that COMP has a notable function in cell behavior and disease progression as a signaling regulator. Interestingly, COMP shows distinct functions in different diseases. Targeting COMP in malignancy may withdraw its beneficial effects on the vascular system and induce or aggravate cardiovascular diseases. COMP supplementation is a promising treatment for OA and aortic aneurysms while it may induce tissue fibrosis or cancer metastasis.
Attenuation of Autism-like Behaviors by an Anthocyanin-Rich Extract from Portuguese Blueberries via Microbiota–Gut–Brain Axis Modulation in a Valproic Acid Mouse Model
Authors: Serra Diana , Henriques Joana F. , Sousa Fábio J. , Laranjo Mariana , Resende Rosa , Ferreira-Marques Marisa , de Freitas Victor , Silva Gabriela , Peça João , Dinis Teresa C. P. , Almeida Leonor M.
Published: August 2022

Autism Spectrum Disorders (ASDs) are a group of neurodevelopmental pathologies whose current treatment is neither curative nor effective. Anthocyanins are naturally occurring compounds abundant in blueberries and in other red fruits which have been shown to be successful in the treatment of several neurological diseases, at least in in vitro and in vivo disease models. The aim of the present work was to study the ability of an anthocyanin-rich extract (ARE) obtained from Portuguese blueberries to alleviate autism-like symptoms in a valproic acid (VPA) mouse model of ASD and to get insights into the underlying molecular mechanisms of such benefits. Therefore, pregnant BALB/c females were treated subcutaneously with a single dose of VPA (500 mg/kg) or saline on gestational day 12.5. Male offspring mice were orally treated with the ARE from Portuguese blueberries (30 mg/kg/day) or the vehicle for three weeks, and further subjected to behavioral tests and biochemical analysis. Our data suggested that the ARE treatment alleviated autism-like behaviors in in utero VPA-exposed mice and, at the same time, decreased both neuroinflammation and gut inflammation, modulated the gut microbiota composition, increased serotonin levels in cerebral prefrontal cortex and gut, and reduced the synaptic dysfunction verified in autistic mice. Overall, our work suggests that anthocyanins extracted from Portuguese blueberries could constitute an effective strategy to ameliorate typical autistic behaviors through modulation of the microbiota–gut–brain axis.
Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding
Authors: Bezborodkina Natalia N. , Stepanov Andrei V. , Vorobev Mikhail L. , Stein Grigory I. , Okovityi Sergey V. , Kudryavtsev Boris N.
Published: August 2022

Glycogen is an easily accessible source of energy for various processes. In hepatocytes, it can be found in the form of individual molecules (β-particles) and their agglomerates (α-particles). The glycogen content in hepatocytes depends on the physiological state and can vary due to the size and number of the particles. Using biochemical, cytofluorometric, interferometric and morphometric methods, the number of β-particles in rat hepatocytes was determined after 48 h of fasting at different time intervals after glucose refeeding. It has been shown that after starvation, hepatocytes contain ~1.6 × 108 β-particles. During refeeding, their number of hepatocytes gradually increases and reaches a maximum (~5.9 × 108) at 45 min after glucose administration, but then quickly decreases. The data obtained suggest that in cells there is a continuous synthesis and degradation of particles, and at different stages of life, one or another process predominates. It has been suggested that in the course of glycogenesis, pre-existing β-particles are replaced by those formed de novo. The main contribution to the deposition of glycogen is made by an increase in the glucose residue number in its molecules. The average diameter of β-particles of glycogen during glycogenesis increases from ~11 nm to 21 nm.
Antimicrobial Peptides with Anti-Candida Activity
Authors: Perez-Rodriguez Aitzol , Eraso Elena , Quindós Guillermo , Mateo Estibaliz
Published: August 2022

Mycoses are accountable for millions of infections yearly worldwide. Invasive candidiasis is the most usual, presenting a high morbidity and mortality. Candida albicans remains the prevalent etiologic agent, but the incidence of other species such as Candida parapsilosis, Candida glabrata and Candida auris keeps increasing. These pathogens frequently show a reduced susceptibility to commonly used antifungal drugs, including polyenes, triazoles and echinocandins, and the incidence of emerging multi-drug-resistant strains of these species continues to increase. Therefore, the need to search for new molecules that target these pathogenic species in a different manner is now more urgent than ever. Nature is an almost endless source of interesting new molecules that could meet this need. Among these molecules, antimicrobial peptides, present in different sources in nature, possess some advantages over conventional antifungal agents, even with their own drawbacks, and are considered as a promising pharmacological option against a wide range of microbial infections. In this review, we describe 20 antimicrobial peptides from different origins that possess an activity against Candida.
Survey on UAV Deployment and Trajectory in Wireless Communication Networks: Applications and Challenges
Authors: Han Sang Ik
Published: August 2022

A new era of the fifth-generation (5G) networks is realized to satisfy user demands on higher data rate and massive connectivity for information sharing and utilization. The vertical applications such as vehicle-to-everything (V2X) communications, industrial automation, smart factory, smart farm and smart cities require ultra-fast communications and wide service range. Coverage extension is a key issue to support the required demands on higher performance, but requires an additional deployment of base or relay stations. Therefore, an efficient solution needs to be cost-effective and easy, in order to deploy more stations. An unmanned aerial vehicle (UAV) has been considered as a candidate to overcome these issues because it is much more cost-effective than the ground stations and does not require network or cell replanning, thereby enhancing the network coverage without additional excessive deployment procedures of the existing networks. UAVs will play important roles in 5G and beyond networks assisting as macro base stations, relay stations, small cells, or a moving aggregator. The performance of UAV wireless networks highly depends on the position or the trajectory of UAVs and the resource managements of entire networks. Recently, there have been extensive studies on performance analysis, UAV deployment, UAV trajectory and resource management of UAV wireless networks to achieve the required demands on performance. This paper surveys research conducted for the UAV deployment and trajectory to construct UAV wireless networks for the coverage extension, the throughput improvement and the resource management for different use cases and scenarios, so as to encourage further studies in this area.
Preliminary Single-Center Experience of Bromelain-Based Eschar Removal in Children with Mixed Deep Dermal and Full Thickness Burns
Authors: Korzeniowski Tomasz , Grywalska Ewelina , Strużyna Jerzy , Bugaj-Tobiasz Magdalena , Surowiecka Agnieszka , Korona-Głowniak Izabela , Staśkiewicz Magdalena , Torres Kamil
Published: August 2022

Introduction: Early eschar removal is the standard management of burns. The goal is to remove all of the necrotic tissue and render the wound suitable for healing or skin grafting. The enzymatic debridement of burn wounds allows for minimally invasive removal of burn eschar. The aim of the study was to describe and compare the demographic characteristics, surgical treatment and outcomes of patients treated with Nexobrid® with patients who had standard surgical excision. Material and Methods: A retrospective review was conducted on children who underwent enzymatic debridement. The study group was compared with children treated with the standard of care (SoC). Results: Twelve children (mean age 8 years, range 3 to 15 years) with mixed deep dermal and full thickness burn wounds were treated with Nexobrid®. The mean size of the burns was 29% TBSA. The median percentage TBSA debrided using Nexobrid® was 15% (range 2–27%). In a clinical assessment, enzymatic debridement was effective in removing dead tissue in a single application. No adverse reaction to Nexobrid® and serious complications after enzymatic procedure were recorded in the study group. The estimated relative risk of the need for reconstructive procedures decreases 3.5 times for the study group (RR 3.5, 95%CI 0.9–13.5, p = 0.089). Conclusion: The bromelain-based enzymatic method offers a good and safe debridement option to improve the treatment and life quality of children with severe burns. The main outcome of interest was the number of reconstructive procedures due to scar contractures, which was reduced in the group treated enzymatically compared to the SoC-treated children.
Comparison of Quality of Life between Patients Undergoing Submental Endoscopic Thyroidectomy and Conventional Thyroidectomy: A Prospective Controlled Clinical Trial
Authors: Piromchai Patorn
Published: August 2022

The objectives of this study were to compare the surgical outcomes and quality of life between patients undergoing submental endoscopic thyroidectomy and those undergoing conventional thyroidectomy. The surgical outcomes and quality of life were recorded. Forty-eight patients were included in the study. Their ages ranged from 20 to 60 years. All patients underwent lobectomy, isthmectomy, or the combination of lobectomy and isthmectomy. Most histological diagnoses were benign (85.42%). The submental endoscopic thyroidectomy group showed better scores in the energy/fatigue, emotional wellbeing, and general health domains (p = 0.006, 0.041, and 0.004, respectively). There were no statistically significant differences in surgical outcomes between the submental endoscopic thyroidectomy and conventional thyroidectomy groups (p > 0.05). Submental endoscopic thyroidectomy is feasible, and permits a better quality of life in terms of the energy/fatigue, emotional wellbeing, and general health domains.
Expression of ABCA1 Transporter and LXRA/LXRB Receptors in Placenta of Women with Late Onset Preeclampsia
Authors: Wolski Hubert , Ożarowski Marcin , Kurzawińska Grażyna , Bogacz Anna , Wolek Marlena , Łuszczyńska Małgorzata , Drews Krzysztof , Mrozikiewicz Aleksandra E. , Mikołajczak Przemysław Ł. , Kujawski Radosław , Czerny Bogusław , Karpiński Tomasz M. , Seremak-Mrozikiewicz Agnieszka
Published: August 2022

Background: Appropriate levels of cholesterol are necessary for the mother and developing fetus, but theirexcess may cause preeclampsia. The ABCA1 transporter mediates the secretion of cholesterol and is highly regulated at the transcriptional level via the nuclear liver X receptors (LXRs). Methods: Sixteen preeclamptic and 39 normotensives healthy women with uncomplicated pregnancies were involved in the case-control study. The placental levels of ABCA1, LXRA and LXRB mRNA were quantified by real-time quantitative PCR. The concentrations of ABCA1, LXRA and LXRB proteins from the placenta were determined using an enzyme-linked immunosorbent assay Results: We found in the logistic regression model significantly lower placental expression of LXRB mRNA (crude OR = 0.26, 95% CI: 0.07–0.94, p = 0.040) and LXRA protein level (crude OR = 0.19, 95% CI: 0.05–0.69, p = 0.012) in late-onset preeclamptic women compared to healthy pregnant women. The values remained statistically significant after adjustment for possible confounders. Conclusions: Our results suggest that high placenta LXRA mRNA and LXRA protein expression levels decrease the risk of late-onset preeclampsia. These nuclear receptors could play a role in the development of preeclampsia through disturbances of lipid metabolism.
Renoprotective Effect of Agalsidase Alfa: A Long-Term Follow-Up of Patients with Fabry Disease
Authors: Cybulla Markus , Nicholls Kathleen , Feriozzi Sandro , Linhart Aleš , Torras Joan , Vujkovac Bojan , Botha Jaco , Anagnostopoulou Christina , West Michael L.
Published: August 2022

Fabry disease is a rare lysosomal storage disorder caused by mutations in the GLA gene, which, without treatment, can cause significant renal dysfunction. We evaluated the effects of enzyme replacement therapy with agalsidase alfa on renal decline in patients with Fabry disease using data from the Fabry Outcome Survey (FOS) registry. Male patients with Fabry disease aged >16 years at agalsidase alfa start were stratified by low (≤0.5 g/24 h) or high (>0.5 g/24 h) baseline proteinuria and by ‘classic’ or ‘non-classic’ phenotype. Overall, 193 male patients with low (n = 135) or high (n = 58) baseline proteinuria were evaluated. Compared with patients with low baseline proteinuria, those with high baseline proteinuria had a lower mean ± standard deviation baseline eGFR (89.1 ± 26.2 vs. 106.6 ± 21.8 mL/min/1.73 m2) and faster mean ± standard error eGFR decline (−3.62 ± 0.42 vs. −1.61 ± 0.28 mL/min/1.73 m2 per year; p < 0.0001). Patients with classic Fabry disease had similar rates of eGFR decline irrespective of baseline proteinuria; only one patient with non-classic Fabry disease had high baseline proteinuria, preventing meaningful comparisons between groups. In this analysis, baseline proteinuria significantly impacted the rate of eGFR decline in the overall population, suggesting that early treatment with good proteinuria control may be associated with renoprotective effects.
The Prevalence and Clinical Impact of Adenomyosis in Pregnancy-Related Hysterectomy
Authors: Orsi Michele , Somigliana Edgardo , Cribiù Fulvia Milena , Lopez Gianluca , Buggio Laura , Ossola Manuela Wally , Ferrazzi Enrico
Published: August 2022

Background: The epidemiology of adenomyosis has been traditionally based on patients undergoing hysterectomy for gynecological indications, while its prevalence among hysterectomies performed for obstetric complications is unknown. The aim of this study was to assess the prevalence and clinical impact of adenomyosis diagnosed through histology among women undergoing pregnancy-related hysterectomy (PH). Methods: This was a retrospective cohort study. Women who delivered at a tertiary care regional obstetric hub in Milan between 2009 and 2020 were reviewed to identify cases of PH. Histopathological reports of surgical specimens were examined. Cases with adenomyosis were compared to those without adenomyosis for baseline characteristics, obstetric history and outcomes. Results: During the study period there were 71,061 births and a total of 130 PH, giving a PH incidence of 1.83 per 1000 deliveries. Adenomyosis cases were 18, giving a prevalence of 13.8%. Adenomyosis was associated with placenta previa (77.8 vs. 45.5%, p = 0.01), chorionamnionitis (27.8 vs. 5.4%, p = 0.008), lower gestational age at birth (32 ± 4.6 vs. 35.5 ± 3.6 weeks’ gestation, p = 0.0004), and intrauterine fetal demise among twin pregnancies (50 vs. 4.5%, p = 0.048). Conclusion: Adenomyosis entails a relevant impact on obstetric and perinatal outcomes related to PH. More evidence is needed on the clinical relevance of an ultrasonographic diagnosis of adenomyosis before conception.
Morphological Changes in Blood Cells in a Rat Model of Heatstroke: A Pilot Study
Authors: Iba Toshiaki , Sawada Tomohiro , Kondo Yutaka , Kondo Kenta , Levy Jerrold H.
Published: August 2022

Despite the increasing threat of heatstroke with global warming, pathophysiologic injury continues to be defined. In addition, morphological changes of the peripheral blood cells in heatstroke have not been well characterized. We evaluated pathophysiologic changes in bone marrow and blood cells in a rat heatstroke model using a 39.5 °C climate chamber. After three hours of incubation, blood and bone marrow samples were collected for morphology, and the direct effects of heat on leukocytes in vitro were evaluated using time-lapse observation. The blood cell count and peripheral/bone marrow smear were examined either in a lethal model (core body temperature exceeded 42.5 °C) or in a sublethal model (<41.5 °C). Significant decreases in platelet and white blood counts occurred in the lethal model (>35% and >20% decreases, respectively) and changes were less in the sublethal model. Platelet clumping with the appearance of large platelets was observed. The neutrophils often demonstrated hyper-segmented nuclei, and lymphocytes showed reactive or blast-like changes. Further, the direct effect of heat on leukocytes noted apoptotic cell death at 41.5 °C, but subsequent necrosis at 43 °C. In summary, our rodent model showed that heatstroke causes platelet aggregation, leukocyte injury, and aponecrotic cell death. Such changes were milder and reversible in sublethal heatstroke. The appearance of immature cells may result from damage to the bone marrow microenvironment. These findings may provide useful information for potential diagnostic and therapeutic considerations.
The Evolution of a Large Biobank at Mass General Brigham
Authors: Boutin Natalie T. , Schecter Samantha B. , Perez Emma F. , Tchamitchian Natasha S. , Cerretani Xander R. , Gainer Vivian S. , Lebo Matthew S. , Mahanta Lisa M. , Karlson Elizabeth W. , Smoller Jordan W.
Published: August 2022

The Mass General Brigham Biobank (formerly Partners HealthCare Biobank) is a large repository of biospecimens and data linked to extensive electronic health record data and survey data. Its objective is to support and enable translational research focused on genomic, environmental, biomarker and family history associations with disease phenotypes. The Biobank has enrolled more than 135,000 participants, generated genomic data on more than 65,000 of its participants, distributed approximately 153,000 biospecimens, and served close to 450 institutional studies with biospecimens or data. Although the Biobank has been successful, based on some measures of output, this has required substantial institutional investment. In addition, several challenges are ongoing, including: (1) developing a sustainable cost model that doesn’t rely as heavily on institutional funding; (2) integrating Biobank operations into clinical workflows; and (3) building a research resource that is diverse and promotes equity in research. Here, we describe the evolution of the Biobank and highlight key lessons learned that may inform other efforts to build biobanking efforts in health system contexts.
What Is Farmers’ Level of Satisfaction under China’s Policy of Collective-Owned Commercial Construction Land Marketisation?
Authors: Liu Jiali , Wang Hengwei
Published: August 2022

The entry of collective-owned commercial construction land into the market is a major reform of China’s land management system, which will help promote the appreciation of rural land, establish a unified urban and rural construction land market, and help rural revitalisation and urban–rural integrated development. Based on the classic customer satisfaction index model, this study constructs a satisfaction model for farmers who enter the market with collective-owned commercial construction land. Farmers’ satisfaction is measured by 7 latent variables and the corresponding 22 observed variables, forming a causal chain containing 13 pairs of interactions. Taking as an example Dazu District of Chongqing City, one of the pilot areas where China’s collective-owned commercial construction land has come on the market, AMOS statistical analysis software is used to test the hypotheses. The research results show that: farmers’ information awareness has the greatest impact on farmers’ satisfaction; the higher farmers’ perceived quality is, the more they can improve their satisfaction; there is a significant relationship between farmers’ satisfaction, farmers’ complaints and farmers’ trust; and the three are closely related. Finally, based on the research results, we try to put forward targeted policy suggestions in order to provide a useful reference for government to push for the promotion of the collective-owned commercial construction land marketisation in other rural areas of China and its future improvement.
The Delivery of mRNA Vaccines for Therapeutics
Authors: Nitika  , Wei Jiao , Hui Ai-Min
Published: August 2022

mRNA vaccines have been revolutionary in combating the COVID-19 pandemic in the past two years. They have also become a versatile tool for the prevention of infectious diseases and treatment of cancers. For effective vaccination, mRNA formulation, delivery method and composition of the mRNA carrier play an important role. mRNA vaccines can be delivered using lipid nanoparticles, polymers, peptides or naked mRNA. The vaccine efficacy is influenced by the appropriate delivery materials, formulation methods and selection of a proper administration route. In addition, co-delivery of several mRNAs could also be beneficial and enhance immunity against various variants of an infectious pathogen or several pathogens altogether. Here, we review the recent progress in the delivery methods, modes of delivery and patentable mRNA vaccine technologies.
Studies on Biological and Molecular Effects of Small-Molecule Kinase Inhibitors on Human Glioblastoma Cells and Organotypic Brain Slices
Authors: Hörnschemeyer Julia , Kirschstein Timo , Reichart Gesine , Sasse Christin , Venus Jakob , Einsle Anne , Porath Katrin , Linnebacher Michael , Köhling Rüdiger , Lange Falko
Published: August 2022

Glioblastoma is the most common and aggressive primary brain tumor. Multiple genetic and epigenetic alterations in several major signaling pathways—including the phosphoinositide 3-kinases (PI3K)/AKT/mTOR and the Raf/MEK/ERK pathway—could be found. We therefore aimed to investigate the biological and molecular effects of small-molecule kinase inhibitors that may interfere with those pathways. For this purpose, patient-derived glioblastoma cells were challenged with dactolisib, ipatasertib, MK-2206, regorafenib, or trametinib. To determine the effects of the small-molecule kinase inhibitors, assays of cell proliferation and apoptosis and immunoblot analyses were performed. To further investigate the effects of ipatasertib on organotypic brain slices harboring glioblastoma cells, the tumor growth was estimated. In addition, the network activity in brain slices was assessed by electrophysiological field potential recordings. Multi-kinase inhibitor regorafenib and both MK-2206 and dactolisib were very effective in all preclinical tumor models, while with respect to trametinib, two cell lines were found to be highly resistant. Only in HROG05 cells, ipatasertib showed anti-tumoral effects in vitro and in organotypic brain slices. Additionally, ipatasertib diminished synchronous network activity in organotypic brain slices. Overall, our data suggest that ipatasertib was only effective in selected tumor models, while especially regorafenib and MK-2206 presented a uniform response pattern.
New Thermo-Reflective Coatings for Applications as a Layer of Heat Insulating Materials
Authors: Malewska Elżbieta , Prociak Aleksander , Vevere Laima , Vanags Edgars , Zemła Marcin , Uram Katarzyna , Kirpluks Mikelis , Cabulis Ugis , Bryk Mirosław
Published: August 2022

This paper presents new thermo-reflective coatings with different properties. Basic, anti-corrosion and self-extinguishing coatings were analyzed. The coatings were obtained with a thickness varying from 1 to 3 mm. The coatings were subjected to detailed tests assessing their physical-mechanical properties, i.e., tensile strength, abrasion, pull-off test, water absorption, vapor permeability and thermal properties, i.e., the thermal performance of the reflective coatings, thermal transmittance, thermogravimetric analysis, differential scanning calorimetry, as well as thermomechanical analysis and thermal conductivity. In addition, the possibility of using such coatings in a wide range of temperatures and during application to various materials used as a substrate, i.e., concrete, metal and rigid polyurethane foam, was tested. The thermal analysis of coatings revealed that materials are stable to temperatures above 200 °C, there are no thermal transitions in the negative temperature region and shrinking in low temperatures is minimal (less than 0.5%). From the data obtained within the framework of this study, it can be concluded that anticorrosive, basic and self-extinguishing coatings are eligible for thermo-insulation applications in temperatures up to 200 °C.
Design and Manufacturing of a Metal-Based Mechanical Metamaterial with Tunable Damping Properties
Authors: Kappe Konstantin , Wahl Jan P. , Gutmann Florian , Boyadzhieva Silviya M. , Hoschke Klaus , Fischer Sarah C. L.
Published: August 2022

In the present work, a novel concept for metallic metamaterials is presented, motivated by the creation of next-generation reversible damping systems that can be exposed to various environmental conditions. For this purpose, a unit cell is designed that consists of a parallel arrangement of a spring and snap-fit mechanism. The combination of the two concepts enables damping properties one order of magnitude higher than those of the constituting metal material. The spring element stores elastic energy while the snap-fit allows to absorb and dissipate energy and to reach a second stable state. Different configurations of single unit cells and connected cell assemblies are manufactured by laser powder bed fusion using Ti6Al4V powder. The dimensioning is supported by finite element modelling and the characteristic properties of the unit cells are studied in cyclic compression experiments. The metamaterial exhibits damping properties in the range of polymeric foams while retaining its higher environmental resistance. By variation of selected geometrical parameters, either bistable or self-recovering characteristics are achieved. Therefore, a metamaterial as an assembly of the described unit cells could offer a high potential as a structural element in future damping or energy storage systems operating at elevated temperatures and extreme environmental conditions.
Coupled Effect of Polypropylene Fibers and Slag on the Impact Resistance and Mechanical Properties of Concrete
Authors: Ali Abdul Basit , Sharif Muhammad Burhan , Irfan-ul-Hassan Muhammad , Iqbal Yasir , Akmal Usman , Alabduljabbar Hisham , Deifalla Ahmed Farouk
Published: August 2022

The disposal of steel slag leads to the occupation of large land areas, along with many environmental consequences, due to the release of poisonous substances into the water and soil. The use of steel slag in concrete as a sand-replacement material can assist in reducing its impacts on the environment and can be an alternative source of fine aggregates. This is the very first paper that seeks to experimentally investigate the cumulative effect of steel slag and polypropylene fibers, particularly on the impact resistance of concrete. Various concrete mixes were devised by substituting natural sand with steel slag at volumetric replacement ratios of 0%, 10%, 20%, 30%, and 40%, with and without fibers. Polypropylene fibers of 12 mm length were introduced into the steel slag concrete at 0%, 0.5%, and 1.0% by weight of cement as a reinforcing material. Performance evaluation of each mix through extensive experimental testing indicated that the use of steel slag as partial substitution of natural sand, up to a certain optimum replacement level of 30%, considerably improved the compressive strength, flexural strength, and tensile strength of the concrete by 20.4%, 23.8%, and 17.0%, respectively. Furthermore, the addition of polypropylene fibers to the steel slag concrete played a beneficial role in the improvement of strength characteristics, particularly the flexural strength and final drop weight impact energy, which had a maximum rise of 48.1% and 164%, correspondingly. Moreover, integral structure and analytical analyses have also been performed in this study to validate the experimental findings. The results obtained encourage the use of fiber-reinforced steel slag concrete (FRSLC) as a potential impact-resistant material considering the environmental advantages, with the suggested substitution, of an addition ratio of 30% and 1.0% for steel slag and polypropylene fibers, respectively.
Modal Analysis Using Digital Image Correlation Technique
Authors: Frankovský Peter , Delyová Ingrid , Sivák Peter , Bocko Jozef , Živčák Jozef , Kicko Michal
Published: August 2022

The present paper discusses a new approach for the experimental determination of modal parameters (resonant frequencies, modal shapes and damping coefficients) based on measured displacement values, using the non-contact optical method of digital image correlation (DIC). The output is a newly developed application module that, based on a three-dimensional displacement matrix from the experimental measurement results, can construct a frequency response function (FRF) for the purpose of experimental and operational modal analysis. From this frequency response function, the modal parameters of interest are able to be determined. The application module has been designed for practical use in Scilab 6.1.0, and its code interfaces directly with the ISTRA4D high-speed camera software. The module was built on measurements of a steel plate excited by an impact hammer to simulate experimental modal analysis. Verification of the correctness of the computational algorithm or the obtained modal parameters of the excited sheet metal plate was performed by simulation in the numerical software Abaqus, whose modal shapes and resonant frequencies showed high agreement with the results of the newly developed application.
Performance Characterisation of a New Plaster Composite Lightened with End-of-Life Tyres’ Recycled Materials for False Ceiling Plates
Authors: Álvarez Manuel , Santos Paulo , Lopes Paulo , Abrantes David , Ferrández Daniel
Published: August 2022

Plaster is one of the most used and studied materials in the building process. This paper shows the result of the characterisation of a new plaster-based material enlightened and reinforced with polymers and end-of-life tyres’ recycled materials. As far as end-of-life tyres are a common waste item, this paper offers new recycling possibilities, as well as significant improvements in new building materials. Mechanical, thermal conductivity, sound absorption, fire reaction and environmental impact are studied and analysed. Three different end-of-life tyres’ recycled materials are used, two size rubber and textile fibres. A significant density reduction up to 17% was achieved mainly due to end-of-life materials lower density. Two thermal conductivity measurement methods, heat flux meter and guarded hot plate, were conducted and then compared. A 20% improvement with respect to the reference was achieved in those samples with textile fibre. The two methods’ measurements got a 1% difference in all samples analysed except textile fibre. Thus, this allowed to validate these methods and assure these measurements. Sound absorption was also measured. These materials reached α = 0.32 in high frequencies. Performance in low frequencies were lower. Fire tests led to no ignition results and no fire propagation. Finally, a basic global warming potential impact study based on environmental product declaration (EPD) is conducted. The most relevant result of this study is the potential 20–34% reduction of CO2 emissions with the elaboration of these composites.
Interaction of Oxicam Derivatives with the Artificial Models of Biological Membranes—Calorimetric and Fluorescence Spectroscopic Study
Authors: Maniewska Jadwiga , Czyżnikowska Żaneta , Szczęśniak-Sięga Berenika M. , Michalak Krystyna
Published: August 2022

The modified 1,2-benzothiazine analogues designed as new drug candidates and discussed in this paper are oxicam derivatives. Oxicams are a class of non-steroidal anti-inflammatory drugs (NSAIDs). Their biological target is cyclooxygenase (COX), a membrane protein associated with the phospholipid bilayer. In recent decades, it has been proven that the biological effect of NSAIDs may be closely related to their interaction at the level of the biological membrane. These processes are often complicated and the biological membranes themselves are very complex. Therefore, to study these mechanisms, simplified models of biological membranes are used. To characterize the interaction of six oxicam derivatives with DPPC, DMPC and EYPC, artificial models of biological membranes (multi-bilayers or liposomes), differential scanning calorimetry (DSC) and fluorescence spectroscopy techniques were applied. In spectroscopic measurements, two fluorescent probes (Laurdan and Prodan) localized in different membrane segments were used. All tested oxicam derivatives interacted with the lipid bilayers and may penetrate the artificial models of biological membranes. They intercalated into the lipid bilayers and were located in the vicinity of the polar/apolar membrane interface. Moreover, a good drug candidate should not only have high efficiency against a molecular target but also exhibit strictly defined ADMET parameters, therefore these activities of the studied compounds were also estimated.
A Seam Tracking Method Based on an Image Segmentation Deep Convolutional Neural Network
Authors: Lu Jun , Yang Aodong , Chen Xiaoyu , Xu Xingwang , Lv Ri , Zhao Zhuang
Published: August 2022

Vision-based welding seam tracking is an important and unique branch of welding automation. Active vision seam tracking systems achieve accurate feature extraction by using an auxiliary light source, but this will introduce extra costs and the real-time performance will be affected. In contrast, passive vision systems achieve better real-time performance and their structure is relatively simple. This paper proposes a passive vision welding seam tracking system in Plasma Arc Welding (PAW) based on semantic segmentation. The BiseNetV2 network is adopted in this paper and online hard example mining (OHEM) is used to improve the segmentation effect. This network structure is a lightweight structure allowing effective image feature extraction. According to the segmentation results, the offset between the welding seam and the welding torch can be calculated. The results of the experiments show that the proposed method can achieve 57 FPS and the average error of the offset calculation is within 0.07 mm, meaning it can be used for real-time seam tracking.
Significance of Convection and Internal Heat Generation on the Thermal Distribution of a Porous Dovetail Fin with Radiative Heat Transfer by Spectral Collocation Method
Authors: Sowmya G. , Lashin Maha M. A. , Khan M. Ijaz , Kumar R. S. Varun , Jagadeesha K. C. , Prasannakumara B. C. , Guedri Kamel , Bafakeeh Omar T , Mohamed Tag-ElDin El Sayed , Galal Ahmed M.
Published: August 2022

A variety of methodologies have been used to explore heat transport enhancement, and the fin approach to inspect heat transfer characteristics is one such effective method. In a broad range of industrial applications, including heat exchangers and microchannel heat sinks, fins are often employed to improve heat transfer. Encouraged by this feature, the present research is concerned with the temperature distribution caused by convective and radiative mechanisms in an internal heat-generating porous longitudinal dovetail fin (DF). The Darcy formulation is considered for analyzing the velocity of the fluid passing through the fin, and the Rosseland approximation determines the radiation heat flux. The heat transfer problem of an inverted trapezoidal (dovetail) fin is governed by a second-order ordinary differential equation (ODE), and to simplify it to a dimensionless form, nondimensional terms are utilized. The generated ODE is numerically solved using the spectral collocation method (SCM) via a local linearization approach. The effect of different physical attributes on the dimensionless thermal field and heat flux is graphically illustrated. As a result, the temperature in the dovetail fin transmits in a decreasing manner for growing values of the porosity parameter. For elevated values of heat generation and the radiation-conduction parameter, the thermal profile of the fin displays increasing behavior, whereas an increment in the convection-conduction parameter downsizes the thermal dispersal. It is found that the SCM technique is very effective and more conveniently handles the nonlinear heat transfer equation. Furthermore, the temperature field results from the SCM-based solution are in very close accordance with the outcomes published in the literature.
Identification of Hair Growth Promoting Components in the Kernels of Prunus mira Koehne and Their Mechanism of Action
Authors: Zhou You , Zhang Jingwen , Chen Wanyue , Li Xiaoli , Fu Ke , Sun Weijun , Liang Yuan , Xu Min , Zhang Jing , Fan Gang , Yin Hongxiang , Wang Zhang
Published: August 2022

The application of the seed oil of Prunus mira Koehne (Tibetan name ཁམབུ།), a plant belonging to the Rosaceae family, for the treatment of alopecia has been recorded in Jingzhu Materia Medica (ཤེལ་གོང་ཤེལ་ཕྲེང་།) (the classic of Tibetan medicine) and Dictionary of Chinese Ethnic Medicine. This study aims to reveal the effective components and mechanism of hair growth promotion in the kernel of Prunus mira Koehne. Network pharmacology was used to predict the mechanism of action and effective components in the treatment of the kernel of Prunus mira Koehne. The contents of amygdalin in 12 batches of the kernel of Prunus mira Koehne were determined by HPLC. An animal model of the depilation of KM mice induced by sodium sulfide was created, and five effective components that promoted hair growth were initially screened. In the study of the effectiveness and mechanism of action, KM and C57BL/6 mice are selected as experimental objects, three screening tests for active components of the kernel of P. mira are performed, and three effective components are screened out from the eight components. HE staining was used to detect the number of hair follicles and the thickness of the dermis. RT-PCR and immunohistochemistry were used to evaluate the influence of the expression of indicators in the Wnt/β-catenin signaling pathway in skin, including β-catenin, GSK-3β, and mRNA and protein expression levels of Cyclin D 1 and LEF 1. The network pharmacology study showed 12 signaling pathways involving 25 targets in the treatment of alopecia by the kernel of Prunus mira Koehne. vitamin E (3.125 mg/cm2/d), β-sitosterol (0.061 mg/cm2/d), and linoleic acid (0.156 mg/cm2/d) in the kernel of Prunus mira Koehne can promote hair growth in mice, and the mechanism of action may be related to the Wnt/β-catenin pathway.
Review on Chemical Constituents of Schizonepeta tenuifolia Briq. and Their Pharmacological Effects
Authors: Zhao Xueying , Zhou Mingwei
Published: August 2022

Schizonepeta tenuifolia Briq. is a famous Chinese traditional medicine with antipyretic, anti-inflammatory, analgesic and hemostatic effects. Many chemical components can be isolated and detected by using various analysis methods, including monoterpenes, sesquiterpenes, aldehydes, ketones, quinones, alcohols, phenols, carboxylic acids and esters, etc., in which volatile oil was considered to be the main chemical component. In this paper, the chemical constituents and their pharmacological effects were reviewed by summarizing the recent literature, revealing the relationship between them.
Regulatory Effects of Metformin, an Antidiabetic Biguanide Drug, on the Metabolism of Primary Rat Adipocytes
Authors: Szkudelski Tomasz , Konieczna Klaudia , Szkudelska Katarzyna
Published: August 2022

Metformin is a biguanide compound commonly applied in humans with type 2 diabetes. The drug affects different tissues, including fat tissue. The direct influence of metformin on cells of fat tissue, i.e., adipocytes, is poorly elucidated. In the present study, the short-term (4-h) effects of metformin on lipogenesis, glucose transport, lipolysis, and lactate release in primary rat adipocytes were explored. It was demonstrated that metformin reduced insulin-induced lipogenesis and increased glucose transport into adipocytes. The tested compound also decreased lactate release from fat cells. It was shown that metformin substantially limited lipolysis stimulated by epinephrine (adrenergic receptor agonist) and dibutyryl-cAMP (direct activator of protein kinase A). Moreover, metformin decreased the lipolytic process triggered by DPCPX (adenosine A1 receptor antagonist). In the case of each lipolytic stimulator, the drug evoked a similar inhibitory effect in the presence of 3 and 12 mM glucose. The lipolytic response of adipocytes to epinephrine was also found to be reduced by metformin when glucose was replaced by alanine. It was demonstrated that the tested compound limits the release of both glycerol and fatty acids from fat cells. The results of the present study provided evidence that metformin significantly affects the metabolism of primary rat adipocytes. Its action covers processes related to lipid accumulation and release and occurs after relatively short-term exposure.
Biotic Elicitors in Adventitious and Hairy Root Cultures: A Review from 2010 to 2022
Authors: Alcalde Miguel Angel , Perez-Matas Edgar , Escrich Ainoa , Cusido Rosa M. , Palazon Javier , Bonfill Mercedes
Published: August 2022

One of the aims of plant in vitro culture is to produce secondary plant metabolites using plant cells and organ cultures, such as cell suspensions, adventitious, and hairy roots (among others). In cases where the biosynthesis of a compound in the plant is restricted to a specific organ, unorganized systems, such as plant cell cultures, are sometimes unsuitable for biosynthesis. Then, its production is based on the establishment of organ cultures such as roots or aerial shoots. To increase the production in these biotechnological systems, elicitors have been used for years as a useful tool since they activate secondary biosynthetic pathways that control the flow of carbon to obtain different plant compounds. One important biotechnological system for the production of plant secondary metabolites or phytochemicals is root culture. Plant roots have a very active metabolism and can biosynthesize a large number of secondary compounds in an exclusive way. Some of these compounds, such as tropane alkaloids, ajmalicine, ginsenosides, etc., can also be biosynthesized in undifferentiated systems, such as cell cultures. In some cases, cell differentiation and organ formation is necessary to produce the bioactive compounds. This review analyses the biotic elicitors most frequently used in adventitious and hairy root cultures from 2010 to 2022, focusing on the plant species, the target secondary metabolite, the elicitor and its concentration, and the yield/productivity of the target compounds obtained. With this overview, it may be easier to work with elicitors in in vitro root cultures and help understand why some are more effective than others.