bluuebunny/crossref_metadata_2025 huggingface dataset:
Mudanças Climáticas e Infestação por Aedes Aegypti na Região Sul do Brasil
Authors: Soek Felipe José , Ferreira Fernanda Evelyn , Klein Martina Victoria , Bauer Natália Chudzik , Caikoski Paulo Sergio , Feltrim Roseghini Wilson Flávio , Mendonça Francisco
Published: July 2023

O presente trabalho propõe estabelecer a relação entre as mudanças climáticas globais e a infestação por Aedes aegypti no Sul do Brasil, tendo como hipótese o aumento na temperatura média anual previstos pelas projeções do Intergovernmental Panel on Climate Change (IPCC) que representa um potencial de ampliação da cobertura territorial do mosquito, vetor da dengue, chikungunya e zika. A metodologia utilizada foi baseada na análise realizada através dos Assessment Reports (AR) publicados pelo IPCC, utilizando-se das projeções climáticas presentes nos Coupled Model Intercomparison Projects (CMIPs) e dos Shared Socio-economic Pathways (SSPs) contendo cenários previstos considerados otimistas (SSP1) e pessimistas (SSP5), e posteriormente foi realizado um levantamento bibliográfico acerca da temática da dengue, enfatizando os seus vetores e sua relação com as condições climáticas ótimas para sua reprodução, tais como a temperatura, umidade e precipitação. Os resultados encontrados apontam para o potencial de ampliação da cobertura territorial do Aedes aegypti a partir da ampliação de territórios e períodos sob condições ótimas de temperatura tanto nas projeções consideradas otimistas quanto nas pessimistas, representando um cenário preocupante para o futuro. Por fim, considera-se que, embora o mosquito seja um vetor de comportamento sinantrópico, as projeções indicam um aumento na sua capacidade de reprodução e de infestação de novas áreas, podendo indicar um risco ao endemismo e ao surgimento de novas epidemias de dengue em regiões ainda não atingidas, além da intensificação destes em regiões endêmicas a partir do aumento dos períodos de tempo sob condição climática ótima à reprodução do vetor.
A existência no fio da navalha: propriedade e violência em Grande sertão: veredas
Authors: A. Barros Vinícius Victor
Published: July 2023

Este artigo discute a complexa relação entre propriedade e violência no âmbito do romance Grande sertão: veredas (1956), de João Guimarães Rosa. Entende-se que, para além da rica matéria social, histórica e política do sertão, uma das principais dimensões dos conflitos narrados se vincula intrinsecamente à concentração de terras por parte dos grandes potentados locais, os coronéis, e pela prática quase institucionalizada do banditismo armado, o jaguncismo. Longe de ser mera fabulação, a análise dialética entre literatura e sociedade almeja sublinhar que o romance de Guimarães Rosa é uma possibilidade de pesquisa e interpretação de um passado não muito distante e que ainda reflete, em maior ou menor grau, determinada lógica de coerções, roubos e assassinatos em áreas características do território brasileiro.
História, resistências e conexões no agrário mexicano: da globalização da agricultura à formação do EZLN
Authors: Da Silva Émerson Neves
Published: July 2023

O presente trabalho analisa a conexão histórica do estabelecimento do Exército Zapatista de Libertação Nacional (EZLN) com o processo de globalização da agricultura no México. A inserção dependente no capitalismo global provocou profundas mudanças tecnológicas e a submissão da agricultura mexicana ao capital financeiro e industrial. Nesse contexto é gestado historicamente o EZLN. A análise utiliza a  abordagem teórica da história global para compreender a interação dialética dos sujeitos sociais com a modernização capitalista da agricultura. O movimento é compreendido enquanto uma experiência de resistência social no território latino-americano, constituída a partir da dialética do conflito, ou seja, da capacidade dos atores sociais mobilizarem saberes populares e científicos no processo de luta social ao enfrentamento das contradições econômicas, sociais e políticas originadas pela globalização da agricultura. Nesse fazer histórico, os zapatistas produziram a identidade coletiva e o ideário do grupo.
Resenha do livro PAIVA, Vasco (2020). O despertar das montanhas. Coimbra: Lápis de Memórias, 2020.
Authors: Vasconcelos Paulo Alexandre
Published: July 2023

Relatos e testemunhos da luta de finais da década de 1960 que rompeu com a "pacatez" do meio rural e das montanhas. Nessa luta os povos da montanha e os agricultores da região do Vouga insurgiram-se com a ação repressiva do Estado Novo português que de forma autoritária, confiscou os terrenos comunais aos povos que deles secularmente beneficiavam.
Legar ao além: uma análise das propriedades da Santa Casa de Misericórdia do Rio de Janeiro
Authors: Machado Karoline Marques
Published: July 2023

O presente artigo visa compreender a relação entre a Irmandade de Nossa Senhora da Misericórdia, como receptora de legados pios, e o imaginário da salvação das almas que possibilitou o acúmulo de bens imóveis pelos irmãos de caridade. As propriedades recebidas pela Misericórdia foram, em sua maioria, amealhadas através de doações estipuladas em testamento por indivíduos que almejavam alcançar o Paraíso. A Misericórdia recebeu o privilégio de manter sob o seu controle alguns dos bens de raiz doados por homens e mulheres que buscavam  a remissão dos pecados ao buscarem a salvação eterna. A correlação entre salvação e caridade representa um caminho para entender o papel da Misericórdia e, principalmente,  como a Irmandade virou uma das principais detentoras de terras urbanas no Rio de Janeiro durante o período colonial.
Os caminhos da luta pela terra: considerações sobre direitos, territórios e noções de “propriedade”
Authors: Pacheco Rosely A. Stefanes
Published: July 2023

No decorrer do processo histórico diversos fatores contribuíram para a situação de violência territorial em que se encontram os povos indígenas no Brasil. Dentre estes povos, destaco os Guarani e Kaiowá, que têm uma trajetória de luta pela posse e demarcação de seus territórios. No trilhar deste “caminho”, deparam-se com inúmeros obstáculos, sendo que alguns dizem respeito às noções de propriedade, terra e território. Neste sentido, este trabalho tem como objetivo apresentar as demandas Guarani e Kaiowá e as noções territoriais que as orientam, pois diferem daquelas embasadas unicamente nos conceitos assentados no direito civilista, o qual identifica o território apenas como uma vasta extensão de terra e que não leva em consideração outras categorias que compõem o território enquanto um espaço para exercer o “bem vive
Prevenção combinada do HIV: estamos diante de um novo paradigma? [Combined HIV prevention: are we facing a new paradigm?] [Prevención combinada del VIH: ¿estamos ante un nuevo paradigma?]
Authors: Fonte Vinícius Rodrigues Fernandes da , Spindola Thelma , Costa Cristiane Maria Amorim , Francisco Márcio Tadeu Ribeiro
Published: July 2023

Objetivo: refletir sobre a estratégia da prevenção combinada do HIV como um novo paradigma de prevenção. Conteúdo: trata-se de uma análise reflexiva do documento norteador da resposta brasileira a epidemia do HIV: “Prevenção combinado do HIV: bases conceituais para profissionais, trabalhadores(as) e gestores(as) de saúde”, proposta pelo Ministério da Saúde do Brasil, à luz do referencial de Thomas Kuhn em sua obra, “A estrutura das revoluções científicas”. O “novo” paradigma, a prevenção combinada, não evidencia mudanças estruturais nas estratégias de prevenção, apenas realiza incorporações de novas tecnologias em sua política. Considerações finais: a prevenção combinada do HIV não se apresenta como um novo paradigma de prevenção na resposta brasileira à epidemia, mas como uma reorganização das estratégias de prevenção dos paradigmas já vigentes.ABSTRACTObjective: to reflect on the combined HIV prevention strategy as a new prevention paradigm. Content: this is a reflective analysis of the guiding document of the Brazilian response to the HIV epidemic: “Combined HIV prevention: conceptual bases for professionals, workers and health managers”, proposed by the Ministry of Health of Brazil, in the light of Thomas Kuhn's reference in his work, "The structure of scientific revolutions". The “new” paradigm, combined prevention, does not show structural changes in prevention strategies, it only incorporates new technologies into its policy. Final considerations: combined HIV prevention is not presented as a new prevention paradigm in the Brazilian response to the epidemic, but as a reorganization of prevention strategies from existing paradigms.RESUMENObjetivo: reflexionar sobre la estrategia combinada de prevención del VIH como nuevo paradigma de prevención. Contenido: se trata de un análisis reflexivo del documento orientador de la respuesta brasileña a la epidemia del VIH: “Prevención combinada del VIH: bases conceptuales para profesionales, trabajadores y gestores de salud”, propuesto por el Ministerio de Salud de Brasil, a la luz de Referencia de Thomas Kuhn en su obra, “La estructura de las revoluciones científicas”. El “nuevo” paradigma, de la prevención combinada, no muestra cambios estructurales en las estrategias de prevención, solo incorpora nuevas tecnologías a su política. Consideraciones finales: la prevención combinada del VIH no se presenta como un nuevo paradigma de prevención en la respuesta brasileña a la epidemia, sino como una reorganización de las estrategias de prevención a partir de los paradigmas existentes.
Orientação educacional e trabalho extraclasse do CAP da UFRJ na revista Escola Secundária (1959-1961)
Authors: Vieira Fernanda Gomes , Dallabrida Norberto
Published: July 2023

O Colégio de Aplicação da UFRJ foi o primeiro a ser criado no país, em 1948, a fim de possibilitar experimentações pedagógicas. Mas somente dez anos depois, foram publicadas as instruções para a implantação das classes secundárias experimentais, que começaria no ano seguinte. Assim, o presente trabalho tem como objetivo analisar a representação do Serviço de Orientação Educacional e das atividades extraclasses das classes secundárias experimentais do Colégio de Aplicação da UFRJ na Revista Escola Secundária (1959-1961), a partir de três artigos sobre a temática presentes na revista. E para a condução teórica desta pesquisa documental e historiográfica, foi usado o conceito de representação a partir do historiador francês Roger Chartier (2002 e 2011) e o conceito de circulação trabalhado por Marta Carvalho (2003). Desse modo, as inovações analisadas das classes experimentais visavam formar a juventude para atender as necessidades da sociedade moderna
Das classes especiais à educação inclusiva: Um estudo sobre o sistema de educação de Miracema/RJ
Authors: Barcellos Danielle Pereira , Gomes Jacqueline De Souza
Published: July 2023

Este estudo reúne relatos sobre o sistema de educação do município de Miracema, compartilhando narrativas de profissionais que atuaram diretamente nas classes especiais e, a partir daí, enumera os principais desafios e as conquistas no salvaguardo do direito à educação para pessoas com deficiência e na implementação da Educação Especial na perspectiva da educação inclusiva em âmbito municipal. Metodologicamente, para além da pesquisa narrativa, utilizamos fontes bibliográficas (em bases selecionadas) e documentais (pertencentes a acervos existentes nos arquivos pessoais dos profissionais de educação que trabalharam nas extintas classes especiais e/ou nas escolas que possuíam tais classes). O objetivo foi abordar como tem se dado a implementação da Política Nacional de Educação Especial na perspectiva da educação inclusiva (2008) no sistema educacional municipal, valendo-nos da percepção de sujeitos envolvidos na passagem das classes especiais segregadoras para a educação inclusiva. Percebemos que, sob a percepção de professores/as, profissionais da Educação, ex-alunos/as e familiares, as melhorias na infraestrutura, capacitação de profissionais e integração dos serviços de saúde e educação ofertados a estudantes com deficiências sempre foram necessários e buscamos compreender se a Educação Especial multidisciplinar e multiprofissional atualmente existente materializa os preceitos de uma Educação Inclusiva na prática.
Establishment of Joint Attention and Parental Cues in Interaction between Hearing Parents and Young Children Who are Deaf and Hard-of-Hearing: A Systematic Review and Meta-Analysis
Authors: Kim Jinju , Lee Youngmee , Lee Gangeun
Published: July 2023

Objectives: This study aimed to identify the differences in the establishment of joint attention in hearing parent-deaf child dyads and hearing parent-hearing child dyads and to investigate the differences in maternal verbal and nonverbal cues during free-play episodes by using a systematic review and meta-analysis. Methods: Fourteen studies that meet the inclusive criteria were selected from eight electronic databases (PubMed, Eric, CINAHL Plus, ScienceDirect, Academic search complete, Google Scholar, RISS, Kyobo Scholar). The effect size was computed using a fixed effects model. Results: In a checklist of the study quality, the selected studies had a low risk of bias, and the subjects of a considerable number of studies were hearing mothers and young children before 36 months of age. Based on a meta-analysis, the two groups had no significant difference in the success rate of joint attention, parental verbal cues, and parental nonverbal cues. Conclusion: We observed a similar success rate of joint attention between hearing parent-deaf child dyads and hearing parent-hearing child dyads. In addition, hearing parents used similar patterns in using verbal and nonverbal cues regardless of their young children’s hearing status. Our results point to important avenues for future research on how hearing parents can better accommodate their children’s hearing status by using verbal and nonverbal cues.
A Study of Validity and Satisfaction of Tele-assessment
Authors: Kim Sung Jin , Kang Jin Kyong , Kim Young Tae , Hong Ki Hyung
Published: July 2023

Objectives: This study aims to examine the validity of two types of remote language screening tests (indirect questionnaire type vs direct observational type), and differences in performance of children as well as satisfaction of their mothers between these two types of remote language screening test. Methods: In total 40 children without any language deficits aged 3 to 6 and their mothers participated in this study. For statistical analysis, a Pearson correlation was conducted between the remote language screening tests (indirect questionnaire type and direct observation type); a PRES (Preschool Receptive-Expressive Language Scale) to find out the concurrent validity, and a paired t-test was conducted to find out if there are significant differences in the performance of the children and satisfaction of their mothers between the two types of screening tests. Additionally as a qualitative analysis, mothers’ preference among two types of the test was asked using an open ended survey. Results: The two types of tests (indirect, direct) showed strong positive correlation with one another. Regarding concurrent validity, both test types showed strong positive correlation with a standardized language test as well. There was no significant difference between the two types in both the performance of the children and the satisfaction of mothers as well. However, 85% percent of mothers preferred the direct observation type citing better accuracy as their reasoning. Conclusion: It is hoped that remote language screening tests can contribute to the implementation of non-face-to-face screening in clinical settings since it is highly validated. Furthermore both two types of tests can be used and complement each other.
Automated detection of lameness in cattle: an update
Authors: Gladden Nicola
Published: July 2023

Lameness is common in the dairy industry, with an estimated prevalence of approximately 30%. Performing regular mobility scoring is recommended on farms to monitor the prevalence of herd lameness and identify (and subsequently treat) lame cows promptly, but mobility scoring can be subjective and may be difficult to regularly implement on farm. Accordingly, there is increasing interest in the automation of lameness detection with direct and indirect methods described. This article provides an overview of automated detection of lameness with a focus on direct methods.
Causes of ovine abortion, vaccination protocols and uptake: an overview
Authors: Jones Charlotte , Charles David
Published: July 2023

Ovine abortion is a worldwide problem for sheep farmers, and can have a variety of infectious and non-infectious causes. The most notable infectious causes are Chlamydia abortus and Toxoplasma gondii, but there are a range of pathogens known to farmers and vets. Diagnosis is achieved by post-mortem examination of aborted lambs and testing of samples including, but not limited to, the foetal membranes and placenta. There are several efficacious vaccines available in the UK targeting abortion-associated pathogens, mainly to be administered before mating. Vaccine uptake is variable, and more emphasis on farmers complying with protocols needed.
Effect of Leukocyte- and Platelet-Rich Fibrin in Postoperative Recovery Following Impacted Mandibular Third Molar Surgery: A Split Mouth Study
Authors: Dos Santos Mateus Veppo , Monteiro Carolina Brito , Goulart Douglas Rangel , Aguiar Silva Isabella Vieira , De Souza Kevin Caio Ferreira , Vieira Frujeri Maria de Lourdes , Matias Micaele da Silva
Published: July 2023

Objective: The surgeries for extraction of impacted lower third molars are more associated with complications such as pain and postoperative edema. The purpose of the present study was to evaluate the impact of the use of Leukocyte- and Platelet-Rich Fibrin (L-PRF) on pain and edema after extraction of impacted mandibular third molars. Material and Methods: A cross-sectional split-mouth study was conducted with 15 volunteer patients who had both impacted mandibular third molars with indication for extraction. The removal surgery was performed one side at a time, with an interval of 14 days. One side received L-PRF while the other did not. Patients were evaluated on the 2nd and 7th postoperative days for mouth opening, pain and edema. Results: In total, data from 14 patients (4 men and 10 women), with a mean age of 21.83 ± 2.8 years were evaluated. There was no statistically significant difference regarding mouth opening, pain and edema on the 7th postoperative day. On the second postoperative day, greater edema was observed in the control group with a statistically significant difference (p = 0.01). Conclusions: Under controlled conditions, the L-PRF does not appear to alter postoperative recovery after extraction of the lower third molars in terms of pain, edema and the ability to open the mouth. Despite the statistically significant difference in edema on the second day, the means were very close and were not clinically relevant.
The Effect of Heat-Polymerizing Method on Color Stability of Acrylic and Composite Resin Denture Teeth
Authors:   Department of Prosthodontics, Faculty of Dentistry, Chulalongkorn University, Thailand, Rochanakit Sindhavajiva Pimrumpai , Angsawad Thanawat ,   Undergraduate student, Faculty of Dentistry, Chulalongkorn University, Thailand, Sengsomwong Thanathip ,   Undergraduate student, Faculty of Dentistry, Chulalongkorn University, Thailand, Tungpid Nussaba ,   Undergraduate student, Faculty of Dentistry, Chulalongkorn University, Thailand
Published: July 2023

Objectives: The purpose of this study was to investigate the color stability of acrylic and composite denture teeth after heat-polymerization method. Methods: Three different brands of acrylic denture teeth: Livera Alpha (LA), Yamahashi (YA) and Major Dent (MA) and 2 different brands of composite denture teeth: Yamahashi PX (YC) and Endura (EC) were tested. Ten maxillary central incisors from each group were pressed onto putty-type polyvinyl siloxane in order to measure color in the same area of denture teeth. Color, at cervical third and incisal third, was recorded by spectrophotometer in CIE L*a*b system. All specimens were undergone heat-polymerization of heat-curing acrylic resin. After flask cooling for 24 hours, the specimens were removed. The color measurement was performed at the same area of each specimen. Color differences (∆E) was calculated. A ∆E of ≤3.3 was considered clinically acceptable. The data were evaluated by 3-way repeated-measures ANOVA and Tukey HSD test (α =0.05). Results: The ∆E of LA, YA, EC, MA and YC at incisal third were 4.41±0.56, 2.45±0.60, 0.76±0.57, 0.75±0.24 and 0.63±0.24 respectively. At cervical third, the ∆E of LA, YA, EC, MA and YC were 4.59±1.26, 2.44±1.08, 0.59±0.36, 0.81±0.61 and 1.22±0.78 respectively. The highest ΔE values were found in LA. These values were significantly higher than those obtained from other brands (p<0.05) and beyond clinically acceptable level. Conclusions: After heat-polymerization, the color of LA changed significantly at both incisal and cervical areas. On the other hand, color stability of YA, EC, MA and YC were clinically acceptable.
Validity of a Simple Periodontal Disease Screening Tool in Thai Patients
Authors:   Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Thailand, Bureesri Tanchanok , Ruengorn Chidchanok ,   Pharmaco-epidemiology and Statistics Research Center (PESRC), Faculty of Pharmacy, Chiang Mai University, Thailand, Sang-in Supatra ,   Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Thailand, Bandhaya Panwadee ,   Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Thailand
Published: July 2023

Objectives: To develop and evaluate the validity of a self-reporting questionnaire for periodontitis in the Thai population. Methods: A cross-sectional analytical study was performed in a group of 300 Thais aged 19-85 years old at the Faculty of Dentistry, Chiang Mai University. Each participant took a questionnaire and received a periodontal status evaluation. Participants were classified into three severity groups using the Centers for Disease Control and Prevention, in partnership with the American Academy of Periodontology (CDC-AAP) criteria. Multivariable ordinal logistic regression analysis was used to achieve the final model. The scoring system was also developed. Results: One hundred patients of each severity group were recruited. Significant predictors in the final model included age, gender, bleeding gums, tooth mobility, dental flossing, dental check-up frequency, diagnosis by a dentist, and individual assessment of periodontitis. The total derived scores identified the severity group of patients into no/mild, moderate, and severe periodontitis. Our model predicted severity groups correctly in 65.7% of patients, while giving an underestimation of 19.3% and an overestimation of 15%. The area under the receiver operating characteristic curves (AuROCs) equal to 0.92 and 0.80 discriminated moderate and severe from no/mild periodontitis, and severe from no/mild and moderate periodontitis, respectively. Conclusions: Our periodontal disease screening tool demonstrates adequate accuracy. Its validity should be evaluated in other populations.
Color Formation on Titanium Surface Treated by Anodization and the Surface Characteristics: A Review
Authors:   Candidate for Master of Science (Dentistry), Faculty of Dentistry, Chiang Mai University, Thailand, Vattanasup Chayanis , Kuntiyaratana Tachakorn ,   Chonburi Dental Center, Thailand, Rungsiyakull Pimduen ,   Department of Prosthodontics, Faculty of Dentistry, Chiang Mai University, Thailand, Chaijareenont Pisaisit ,   Department of Prosthodontics, Faculty of Dentistry, Chiang Mai University, Thailand
Published: July 2023

Tooth loss is a common problem found in human beings. The challenge in replacing teeth is to restore the natural tooth appearance. Dental implants have overcome this challenge. Titanium (Ti) is an attractive material for dental implants due to its excellent properties and biocompatibility, but the grayish appearance is a concern. A process of supplying the current voltage to an electrochemical cell forming the oxide layer on metal is called anodization. Anodization is a surface modification technique that creates micro- and nano-roughness and an oxide layer on the Ti surface, improving the properties of dental implants. The higher the voltage applied to the anodizing process, the higher the anodic film thickness forms. The different thicknesses of titanium oxide (TiO2) film cause variations in refractive index and reflective index, resulting in a desired color on the Ti surface. The most useful colors for dental application have been gold (10 V), yellow (60 to 65 V), and pink (70 to 80 V) in hue. Yellow and gold hues were mostly used in restoration or abutment areas while pink hue was used for gingival areas. The purposes of this literature review are to assess the current knowledge on the anodization process of titanium and its characteristics and to provide an overview of the literature on color formation on Ti surfaces by anodization.
Comparison of Medical Student Syndrome Among Medical Students in Karachi, Pakistan
Authors:   Community Medicine Department, Jinnah Sindh Medical University, Pakistan, Zaidi Tafazzul Hyder , Zafar Mubashir ,   Family and Community Medicine Department, College of Medicine, University of Hail, Kingdom of Saudi Arabia., Zaidi Nadira Hyder ,   Shaheed Zulfiqar Ali Bhutto Institute of Science And Technology, Pakistan, Khan Muhammad Asad Ali ,   Community Medicine Department, Jinnah Sindh Medical University, Pakistan, ur-Rafe Syed Atta ,   Community Medicine Department, Jinnah Sindh Medical University, Pakistan, Aqsa Hira ,   Community Medicine Department, Jinnah Sindh Medical University, Pakistan, Asfhaq Syeda Ayesha ,   Community Medicine Department, Jinnah Sindh Medical University, Pakistan, Ramadan Rida ,   Community Medicine Department, Jinnah Sindh Medical University, Pakistan, Fazal Memoona ,   Community Medicine Department, Jinnah Sindh Medical University, Pakistan
Published: July 2023

Hypochondriasis, otherwise known as Medical Student Syndrome, is a common diagnosis for students of medicine. The objective of this study is to compare the prevalence of Medical Student Syndrome among pre-clinical and clinical year medical students at public sector medical schools in Karachi, Pakistan. A cross-sectional study was conducted at a public sector medical school. A total of 316 students were selected through multistage stratified random sampling. A validated and structured questionnaire was used for data collection. Any student with a known psychiatric disorder was excluded from the study. Data, with a chi-square test, determined the difference in syndromes between pre-clinical and clinical years of students. A p-value of 0.05 was considered statistically significant in the analysis. We found that Medical Student Syndrome is more prevalent among clinical year students than pre-clinical students and this difference is statistically significant (Mean Rank: 23.6 2.42 vs. 29.8 1.98, p-value 0.005). Differences in clinical and pre-clinical year students related to different items of the anxiety scale, such as stress affects, physical and mental health (P-value-0.032), and disease interfering with daily activity (P-value-0.031). Clinical year students are more likely to self-medicate, seek psychiatric counseling, and be concerned about their health. There is need for a counseling system which will reduce the burden of this syndrome. Keywords: Anxiety, Hypochondriasis, Medical Student Syndrome.
Green Synthesis of Fe3O4 Nanoparticles Using Green Betel Leaf Extract for Methylene Blue Adsorption
Authors:   Department of Physics, Faculty of Mathematics and Science, Universitas Negeri Surabaya, Indonesia, . Khoirotin , Faaizatunnisa Nuhaa ,   Department of Chemistry, FASAD, Institut Teknologi Sepuluh Nopember (ITS), Surabaya, Indonesia, . Munasir ,   Department of Physics, Faculty of Mathematics and Science, Universitas Negeri Surabaya, Indonesia
Published: July 2023

Abstract The synthesis of Fe3O4 nanoparticles has become a matter of interest because it has properties and useful applications in various fields. Using plant extracts using the green synthesis method to manufacture Fe3O4 nanoparticles is considered an effective, efficient, and environmentally friendly approach because it does not contain harmful chemicals. This study uses the green synthesis method to synthesize Fe3O4 nanoparticles from green betel leaf extract. The UV-Vis Fe3O4 nanoparticles showed continuous absorption at wavelengths between 300 nm and 800 nm and obtained a band gap energy of 1.87 eV. XRD analysis confirmed the crystal size of Fe3O4 nanoparticles (≈ 6.93 nm). The FTIR results show that functional groups from the leaf extract play a role in forming Fe3O4 nanoparticles, as evidenced by the presence of two Fe-O absorption bands at wave numbers 567 cm-1 and 482 cm-1. The SEM results showed a needle-like particle shape, the HR-TEM analysis showed spherical and needle-like shapes and nanoparticles size (≈ 29.82 nm) and the EDS analysis showed that Fe and O elements indicated the dominant iron oxide content of Fe3O4 with levels of 73.35% and 20.88%, respectively. The magnetization curve shows that the green synthesized magnetite has ferromagnetic properties. The ferromagnetic properties of Fe3O4 were then applied as methylene blue adsorbent and observed using UV-Vis spectroscopy, the most effective absorption ability was at a contact time of 120 minutes with a color degradation percentage of 98.75%. Keywords: Green synthesis, Fe3O4, Green betel, Adsorption, Methylene blue
Mouse Monoclonal Antibody Specific To Human Ena Blood Group Antigen
Authors:   Division of Transfusion Science, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand, Wutti-in Yupanun , Vongchan Preeyanat ,   Division of Transfusion Science, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Published: July 2023

Abstract The aim of this study was to produce mouse monoclonal antibodies (mAbs) specific to human blood group antigens. Two percent solution of normal human red blood cells (RBCs) O, RhD+ in normal saline was freshly prepared, and 100 uL was immunized weekly into BALB/mice, intraperitoneal route for 3 weeks. Prior to each immunization, 100 uL of mouse blood was collected and serum was separated. The rising of anti-RBCs antibody titer was performed by standard hemagglutination compared to pre-immunized serum. Mouse was then sacrificed, and the spleen was collected. The fusion between mouse spleen cells and mouse myeloma (X63Ag8.653) cell lines was performed according to the standard hybridoma technique. Hybrid clones were grown in DMEM high glucose supplemented with 20% FCS at 3 °C, 5% CO2 incubator with 5% humidity. The anti-RBCs in hybrid cell culture supernatant were tested by standard hemagglutination and a monoclonal antibody-producing single clone of the hybrid cell was generated by limiting dilution technique. Antibody screening and identification were performed using screening cells, panel cells, and rare blood type RBCs, followed by enzyme treatment technique. The Isotype of mAb was also identified. The results showed that amongst those obtained, mAb clone 1E10-2B2, was confirmed to be anti-Ena characterized as ficin resistant (anti-EnaFR) and isotype IgG2b, kappa. The anti-Ena mAb is very helpful and can be applied as specific mAb for the phenotyping of Ena which is the high-frequency blood group antigens in all populations. Keywords: Glyphorin, MNS blood group, Ena, alloantibody, HDFN, HTR
Gonad Homogeneity, Maturity, and Gonado-Somatic Index of Wild Indonesian Eel (Anguilla bicolor bicolor McClelland, 1844)
Authors:   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia, Retnoaji Bambang , Prasetyo Adhi ,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia, Septriani Nur Indah ,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia,   Faculty of Agriculture, Kyushu University, Fukuoka, Japan, Sofyantoro Fajar ,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia, Mochioka Noritaka ,   Faculty of Agriculture, Kyushu University, Fukuoka, Japan
Published: July 2023

Abstract The current study was conducted to investigate the homogeneity of gonad maturity in Anguilla bicolor bicolor, and whether Gonado-Somatic Index (GSI) is a reliable indicator of this maturity. Eel samples were obtained from fishermen at Segara Anakan, Cilacap, Central Java, Indonesia in December 2015, and May 2017. Fourteen specimens were analyzed based on their gonad histological structure from cranial, medial, and caudal portions and were measured for GSI values. The present study shows that there is a synchronization of oocyte maturity time on the medial, cranial and caudal parts. It might suggest that during the maturation process, Anguilla develop into silver eels and return to the sea to spawn. The highest GSI value is 2.67 in the female eel containing the highest number of mid-vitellogenic oocytes. Based on these results, it can be suggested that GSI are a suitable parameter for measuring the gonadal maturity of female A. bicolor bicolor. Keywords: Gonadal maturity, Homogeneity, Indonesian eel, A. bicolor bicolor, Segara Anakan
Novel Denture Cleanser Formulated From Virgin Coconut Oil and The Anionic Emulsifier Against Candida albicans Biofilms Formed on 96-Wells Plate and Acrylic Resin Surfaces
Authors:   Department of Family and Community Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand, Siriyod Wariya , Wanachantararak Phenphichar ,   Dental Research Center, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand, Sastraruji Thanapat ,   Dental Research Center, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand, Chaijareenont Pisaisit Chaijareenont ,   Department of Prosthodontic, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand, Chaiyana Wantida ,   Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand, Sookkhee Siriwoot ,   Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, Owittayakul Darunee ,   Department of Family and Community Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand
Published: July 2023

Abstract The objectives of this study were to investigate the inhibitory effect of coconut oil denture-cleansing (CDC) formula against Candida albicans biofilm formed on 96-wells plate and acrylic surfaces at various immersion times and to determine the effects on physical properties of heat-cured acrylic surfaces. A total of twenty-seven CDC formulas were prepared. All CDC formulas were evaluated the stability and anticandidal activity by freeze-defrost cycles and broth microdilution assays, respectively. The formula 22th (F22) containing 0.3 g of anionic emulsifier at a ratio 40:60 of virgin coconut oil to distilled water showed the highest stability and anticandidal activity. The percent inhibition against C. albicans biofilm formed on 96-well plate and acrylic resin surfaces of the F22 after 8 hours of immersion were 86.87 ± 0.65%, and 91.19 ± 1.81%, respectively. However, the F22 had significantly lower inhibitory activity than 0.12% chlorhexidine gluconate (P <0.05). Furthermore, flexural strength of acrylic resin specimens was determined using a 3-point bending test and surface roughness was measured with a profilometer. The flexural strength and the change in surface roughness of F22 were not significant different compared with chlorhexidine and distilled water after 8 hours immersion for 30 days (P >0.05). In conclusions, F22 which containing 0.3 g of anionic emulsifier at the ratio of coconut oil to distilled water at 40:60 exhibited the potent inhibitory activity to Candida albicans biofilms and had no significant effect on the flexural strength and surface roughness of acrylic resins after immersion for 30 days. Keywords: Denture cleanser, Coconut oil, Anionic emulsifier, Biofilm, Candida albicans, Acrylic resins
Pesticide Residues Detection in Agricultural Products
Authors:   Graduate School of Engineering in Agricultural Engineering, Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, 50200, Thailand, Manggala Braja , Chaichana Chatchawan ,   Renewable Energy and Environment Conservation Laboratory, Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, 50200, Thailand, Syahputra Wahyu Nurkholis Hadi ,   Graduate School of Engineering in Agricultural Engineering, Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, 50200, Thailand, Wongwilai Wasin ,   Renewable Energy and Energy Efficiency Research Unit, Science and Technology Research Institute Chiang Mai University, Chiang Mai, 50200, Thailand
Published: July 2023

Abstract Pesticides have been the most often used substance in recent decades to protect agricultural goods from pests affecting farmers, especially in conventional agriculture. Pesticides are effective in preventing and removing pests. On the other hand, pesticides risk human health since they may be found in agricultural goods for an extended time. As a result, it is critical to have a robust analytical procedure in place to monitor pesticide residues in agricultural products. Chromatography, Raman spectroscopy, and Ultraviolet-visible (UV-VIS) - Near Infrared (NIR) are methods used to identify pesticide residues, and each has benefits. Additionally, a cutting-edge technique called hyperspectral imaging has recently been employed. This review paper discusses the most current application of those approaches, combined with machine learning and chemometrics, in identifying pesticide residues in agricultural goods such as crops, vegetables, and fruits. The approach's basic principles, benefits, and drawbacks will be briefly addressed. Our findings indicate that those methods provide precise and stable results for identifying pesticide residues in agricultural products. However, most of those methods are possessed a high initial cost, complex processes, time-consuming, which is inappropriate with the agricultural modern concept, especially related to smallholder farmers. Hence, shortly, a low-cost, portable, and highly accurate internet-connected device must be developed. Keywords: Pesticide residue, Chromatography, Raman spectroscopy, UV-VIS-NIR spectroscopy, Hyperspectral imaging
Efficacy of Knee Acupuncture added Sanyinjiao Point for Patients with Knee Osteoarthritis Through Attenuated Systemic Inflammations
Authors:   Department of Traditional Chinese Medicine, School of Public Health, University of Phayao, Phayao 56000, Thailand, Klajing Warangkhana , Sumneang Natticha ,   Department of Medical Science, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand, Tan Yin Shen ,   Department of Traditional Chinese Medicine, School of Public Health, University of Phayao, Phayao 56000, Thailand, Jaengpromma Pongnared ,   Department of Traditional Chinese Medicine, School of Public Health, University of Phayao, Phayao 56000, Thailand, Kobroob Anongporn ,   Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand
Published: July 2023

Abstract Osteoarthritis (OA) is a progressive degradation of the cartilage that is strongly linked to the inflammation, and resulting in pain, stiffness, and loss of mobility. Currently, interventions aimed at reducing inflammation, alleviating pain, and enhancing joint mobility are recommend in patients with OA. Although intervention with traditional acupuncture has been shown to reduce pain, inflammation, and improve knee motion, the effects of acupuncture added Sanyinjiao (SP6) point on pain, inflammation and knee motion have never been investigated in patients with knee OA. Twenty-three patients with knee OA were subjected to receive acupuncture added SP6 twice a week for five weeks (totaling ten sessions). Patients were assessed at baseline and after ten sessions of acupuncture added SP6 using knee range of motion measurement, modified Western Ontario and McMaster Universities OA Index, numerical rating scale, and systemic inflammation levels. After the end of treatment, the knee pain was significantly reduced in patients when compared to baseline (P < 0.001). Addition to improvement in knee pain, knee flexion and extension also showed significant improved compared to baseline (P < 0.001). Furthermore, the low-grade systemic inflammation levels were decreased in patients when compared to baseline (P < 0.001). Therefore, this is the first study to show that acupuncture added SP6 point provides the beneficial effects on pain and inflammation, improving knee motion in patients with knee OA. Keywords: Acupuncture, Inflammation, Knee motion, Knee osteoarthritis, Pain, Chinese medicine
Effect of locally available and commercial preservatives on nutrient content, organic matter digestibility and microbial changes of wet brewers' grain
Authors:   School of Animal and Range Sciences, College of Agriculture, Hawassa University, P.O.Box 5, Hawassa, Ethiopia, Abdu Yunus ,   Department of Animal Sciences, , College of Agriculture and Environmental Science, Arsi University, P.O.Box 193, Asella, Ethiopia, Tolera Adugna ,   School of Animal and Range Sciences, College of Agriculture, Hawassa University, P.O.Box 5, Hawassa, Ethiopia, Nurfeta Ajebu ,   School of Animal and Range Sciences, College of Agriculture, Hawassa University, P.O.Box 5, Hawassa, Ethiopia, Bradford Barry ,   Departments of Animal Science, Michigan State University, East Lansing, MI, USA
Published: July 2023

The aim of the study was to evaluate the effect of different preservatives on nutrient content, organic matter digestibility (OMD), ammonia nitrogen (NH3-N), short-chain fatty acid (SCFA), yeast, mold, and lactic acid bacteria (LAB) concentrations of wet brewery grain (WBG). The experiment was undertaken for 28-days using five treatments (T); T1 = no preservative; T2 = 1.75% salt; T3 = 2.5% molasses; T4 = 2.5% Effective Microbial (EM) and T5 = 0.6% formic acid. Data was analyzed using a completely randomized two way factorial design of the mixed procedure of SAS (Version 9.1, 2001). The highest dry matter loss was for WBG stored with T1, while the lowest was for WBG treated with T5. WBG treated with T5 had the highest crude protein content, while those with T1 had the lowest. OMD of WBG treated with T5 was higher than T4. SCFA content of WBG treated with T3 was higher than those T4. WBG stored with T1 and T3 had a higher NH3N content than T4 and T5. WBG stored with T1 had the highest yeast and mold concentration, while WBG treated with T5 had the least. The highest LAB count was recorded for WBG treated with T5, while the least was for T1 and T3. Results has shown that preserving WBG with 0.6% formic acid is found to be more effective in terms of preventing spoilage by inhibiting mold and yeast growth as compared to other preservatives used in this study.
Classification and correlation of coughing sounds and disease status in fattening pigs
Authors:   Department of food animal disease, Faculty of veterinary medicine, Chiang Mai university, Chiang Mai 51000 Thailand, Yamsakul Panuwat , Yano Terdsak ,   Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 51000 Thailand, Na Lampang Kannika ,   Department of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand, Khamkong Manad ,   Department of Statistics, Faculty of Science, Chiang Mai University, Chiang Mai 50100, Thailand, Srikitjakarn Lertrak ,   PODD center, Chiang Mai University, Chiang Mai 50100, Thailand
Published: July 2023

There have been attempts to use technology to distinguish pig coughs from other sounds on farms. Machine learning is being used to classify pig coughs via python. Sound files have been converted to images that are composed of wave plots,spectrograms and log power spectrograms for identification of those sounds. A recorder was used with a total of 45 healthy three-bred weaned piglets, wherein three replications of each were used with 15 weaning pigs per pen during different months. This process was set up within the housing unit at a ratio as 1:1 (recorder per pen). Sounds, blood samples and tonsil swabs were collected every month. Pig cough sounds were then classified from other sounds and a coughing index (CI) was established. Blood samples and tonsil swabs were utilized to determine respiratory diseases via laboratory tests that included ELISA, PCR and bacterial cultures. According to our results, pig coughs sound distinctly different from other sounds as had been classified by python. Moreover, the laboratory results of the seroprofile of Mycoplasma hyopneumoniae (M.hyo), Porcine Reproductive and Respiratory Syndrome virus (PRRSv), and Porcine Circovirus type 2 (PCV2), as was established by ELISA test, were employed in disease detection during the fattening period. Spearman rank correlations and Kappa analysis were used to establish correlation values between coughing and the results of laboratory tests. CI revealed a high correlation coefficient and agreement with the ELISA results of M.hyo, as well as the PCR results of PRRSv and PCV2 (p<0.05), while CI also revealed low correlation coefficient and agreement with the results of the Streptococcus spp. and Pasteurella spp. cultures (p>0.05). Therefore, the monitoring of coughing can be suited to detect respiratory problems and any potential relationships with M.hyo, PRRSv and PCV2 infections