bluuebunny/crossref_metadata_2025 huggingface dataset:
Rancang Bangun Alat Ukur Konsentrasi Gas Sulfur Dioksida (SO2) Berbasis Mikrokontroller Dan Sensor MQ136
Authors: Kasenda Dewi I.A , Suoth Verna Albert , Mosey Handy I.R
Published: July 2019

Telah dilakukan penelitian untuk merancang-bangun alat ukur konsentrasi gas Sulfur dioksida ( ) berbasis mikrokontroller dan menggunakan Sensor MQ136. Tujuan mengapa Sulfur dioksida sangat penting diukur karena sulfur dioksida  memiliki sensitivitas individu yang berdampak pada kesehatan manusia seperti mengiritasi mata, tenggorokan, dan saluran pernapasan. Hasil penelitian menghasilkan alat pengukur sensor dengan pengujian menunjukan bahwa sensor dapat bekerja dengan baik ketika membaca konsentrasi gas pada ruang tertutup dengan nilai   sebesar 0,985. Pengujian pada ruang terbuka akan menyebabkan fluktuasi pembacaan sensor karena dipengaruhi oleh beberapa faktor lingkungan seperti angin, suhu dan kelembaban. Untuk menghindari fluktuasi ini dapat dilakukan dengan cara meletakan sensor pada ruang tertutup sedemikian rupa sehingga tidak terlalu terpengaruh oleh faktor lingkungan. Dari hasil pengukuran tersebut disimpulkan bahwa prototipe alat pengukurThe research has been carried out to design-build a concentration gauge of sulfur dioxide ( ) based on a microcontroller and use MQ136 sensors. The purpose of sulfur dioxide is very important to measure because sulfur dioxide has individual sensitivity that has an impact on human health such as irritating the eyes, throat and respiratory tract. The results of the study produced a sensor measuring device with testing showing that the sensor can work well when reading the concentration of gas in a closed space with a value of of 0.985. Testing on open spaces will cause fluctuations in sensor readings because they are influenced by several environmental factors such as wind, temperature and humidity. To avoid these fluctuations, it can be done by placing sensors in closed spaces in such a way that they are not too affected by environmental factors. From the results of these measurements it was concluded that the gas concentration meter prototype had worked well when operated
Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)
Authors: Melsadalam Florentin Natalia , Katja Dewa Gede , Sangi Meiske S.
Published: July 2019

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria
Kandungan Klorofil Total Daun Pada Padi Lokal Sulawesi Utara Yang Mengalami Cekaman Banjir
Authors: Tjolleng Fitria , Siahaan Parluhutan , Ai Nio Song
Published: July 2019

Penelitian ini bertujuan untuk mengkaji respon fisiologi tanaman padi lokal Sulawesi Utara (varietas Ombong, Temo, TB, dan Sultan) terhadap cekaman banjir pada fase vegetatif berdasarkan kandungan klorofil total pada daun. Pot dan tanaman padi dimasukkan ke dalam ember sehingga terendam setinggi 27 cm di atas permukaan media. Perlakuan cekaman kebanjiran berlangsung selama 20 hari. Pengambilan data dilakukan pada hari ke-0 (sebelum perlakuan), 10, 15 dan 20 setelah perlakuan. Daun diambil sebanyak 1 g kemudian dihaluskan dan diekstraksi menggunakan alkohol 95% dan kandungan klorofil diukur dengan spektrofotometer. Data kandungan klorofil total dalam percobaan faktorial dianalisis dengan analisis sidik ragam dalam rancangan acak lengkap pada tingkat kepercayaan 95%. Hasil penelitian ini menunjukkan bahwa faktor varietas, faktor waktu dan interaksi antara keduanya tidak menunjukkan perbedaan yang nyata pada kandungan klorofil total pada daun tanaman padi (varietas Ombong, Temo, TB, dan Sultan) selama 20 hari perlakuan cekaman banjirThis study examined the physiological responses of North Sulawesi local rice plants in (Ombong, Temo, TB, and Sultan varieties) to partial submergence at the vegetative phase based on the content of total chlorophyll in the rice leaves. The pot and plants were submerged under water in a bucket about 27 cm above the surface of the media. The submergence treatment was lasted for 20 days. Data collection was carried out at day 0 (before treatment), 10, 15 and 20 after treatment. The leaves (about 1 g for ach sample) were collected, then refined and extracted using 95% ethanol. The total chlorophyll content was measured using spectrophotometer. Data of total chlorophyll content in the factorial experiment were analyzed using analysis of variance under completely randomized design at a 95% confidence level. The results of this study indicated that variety and time factors as well as their interactions showed no significant differences in the content of total chlorophyll in the leaves of rice plant varieties (Ombong, Temo, TB, and Sultan) during 20 days of partial submergence
Pertumbuhan Cladocera jenis Chydoridae pada media kultur yang berbeda
Authors: Sambode Denovis , Pangkey Henneke , Lantu Sartje
Published: July 2019

The aim of this study was to find out the effect of different organic fertilizers on the population growth of Chydorus sp. This research was conducted at Laboratorium of Nutrition and Food Technology, Faculty of Fishery and Marine Sciences, Sam Ratulangi University, from December, 2012 to January, 2013. The experiment was run in the Completely Randomized Design with 3 treatments and 3 replication. Chydorus sp. was cultured in 9 glass jars with a volume of 1 L each. Three treatments applied in this research included A: medium culture composed of 500ml of water and 50 grams of soil; B: medium culture composed of 500ml of water, 50 grams of soil and 10g of cow dung; C: medium culture composed of 500ml of water, 50 grams of soil and 10g of horse dung. The density of Cydorus sp. in each media was 10 individuals /500 ml water. Observation on the population growth, development of live preys, and water quality parameters was conducted for 20 days period. Data were statistically analized with Analysis of variance. The results showed the highest density of Chydorus sp. was reached by Chydorus cultured in medium with horse manure (2169 individual/500 ml), followed by medium culture with cow manure (1715 individual/500 ml), and the lowest in medium culture with soil which was 1065,33 individual/500 ml. However, Analysis of variances showed that Chydorus population growth were not significantly affected by different culture medium. Keywords: manure, liquid fertilizer, growth, Chydorus sp.
Kualitas Fisika-Kimia Air di Areal Budidaya Desa Kaima, Eris dan Toulimembet, Kabupaten Minahasa, Provinsi Sulawesi Utara
Authors: DeBreving Zainal M. , Rompas Robert J.
Published: July 2019

ABSTRACT Water physical-chemical quality at fish farming area in Kaima, Eris and Toulimembet villages around Lake Tondano was observed. Water quality parameters including temperature, pH, brightness and dissolved oxygen were measured in situ. Observation was conducted at three stations, which were waters around Kaima, Eris, and Toulimembet villages. Measurement was carried out at 2-points at each station, where appointing of both points was based on the consideration of the activities of fish farming and settlement layout. Point-1 represented the activities of fish farming whereas point-2 represented the settlement activity. Data were collected at one week interval. Results showed that water temperature ranged from 26 - 27 0C; brightness was above 2 m; dissolved oxygen ranged from 2.5 - 8.3 ppm and pH ranged from 7,9 - 8,7. Based on the water quality criteria for aquaculture, water quality parameters on fish farming area in Kaima, Eris and Toulimembet, were still suitable for fish farming activities. Keywords: Lake Tondano, water quality, fish farming
Pengaruh beberapa jenis pakan hijauan terhadap pertumbuhan ikan Koan Stenopharyngodon idella
Authors: Babo Desmianti , Sampekalo Julius , Pangkey Henneke
Published: July 2019

The aim of this research was to determine effect of feed plants on growth of grass carp, Stenopharyngodon idella. Grass carp used had an average length of 12.8 cm and weight of 21.00 g. Fish were kept in 12 pouch nets with a density of 8 fish/net. Fish was fed in et libithum, twice a day. This research used Completely Randomized Design with four treatments: A Pennistum purpureum, B Eichornia crassipes, C Pistia stratiotes and D Azolla pinnata, each with three replications. Data from each treatment were statistically analyzed using analysis of variance and LSD. Research results showed that growth of fish in treatment C was significantly different as compared to other treatments. Length of fish reached 3.30 cm, absolute growth 22.12 g and relative growth achieved 103.60%. thus, application of feed plant Pistia stratiotes might improved growth of grass carp.
Evaluasi Kualitas, Kuantitas Telur Dan Larva Ikan Patin Siam (Pangasianodon Hiphopthalmus) Dengan Penambahan Ovaprim Dosis Berbeda
Authors: Manantung Vina O , Sinjal Hengky J , Monijung Revol D
Published: July 2019

This study was conducted to determine the effect of different doses of ovaprim on latency time of spawning, hatching rate, and survival of fish larvae (Pangasianodon hiphopthalmus). Experimental design used was Randomized Complete Design (CRD) with four treatments, each with three replication. The treatments including 0 ml, 0.3 ml, 0.6 ml and 0.9 ml ovaprim per kg of body weight of fish. Data collected consisted of latency time of spawning, hatching rate, and survival rate of larvae. The results showed that treatment with different doses of ovaprim hormones resulted in a significant influence on latency time of spawning, egg hatchability and survival rate of larvae. It was found that 0.6 ml ovaprim could increase the latency time of spawning, egg hatchability and survival rate of larvae. The average latency time of larvae was 529 minutes, hatching rate was 80,86% and survival rate of larvae was 83,33%.
Molecular identification of pathogenic bacteria and PCR specific primer design
Authors: Aris Muh. , Sukenda Sukenda , Harris Enang , Sukadi Muh. Fatuhcri
Published: July 2019

Management of healthy seaweed aquaculture and control of ice ice disease are important component in seaweed production. To support the integrated prevention of ice ice disease, information about genetic variation of bacterial pathogen and the availability of fast and accurate detection are required. This study aimed to identify bacterial pathogen based on gene sequence analysis 16S-rRNA, construction of specific PCR primer from gene sequent analysis 16S-rRNA from bacteria that had the highest pathogenicity. Gene 16S rRNA of bacteria that had the highest pathogenicity was amplificated with universal primer PCR domain forward primer 63f (5’-CAG GCC TAA CAC ATG CAA GTC-3’) and reverse primer 1387r (5’-GGG CGG WGT GTA CAA GGC-3’). DNA Sequence obtained was compared to data base European Bioinformatics Institute (EBI) BLASTN. Construction and feasibility analysis of primer pair was done using primer 3 program. Two specific primer PCR were successfully constructed namely aSEFM-F (5- CAGCCACACTGGAACTGAGA-3) and aSEFM-R(5 TTAGCCGGTGCTTCTTCTGT -3). Both primer reacted optimum at 60°C and produced 201 bp amplicon. Keywords: pathogenicity, gene 16S-rRNA, PCR, primer, specific
The growth of Kappaphycus alvarezii under different depth and initial weight in Talengen waters, Sangihe Islands Regency
Authors: Tiwa Richie B , Mondoringin Lukas L.J.J , Salindeho Indra N
Published: July 2019

The effect of depth and weight differences on early stage of seaweed growth Kappaphycus alvarezi was studied. This Research was conducted for over 42 days, from April to May 2013 in Talengen Waters, Regency Sangihe Islands, North Sulawesi. Experiment designed used was 4 x 3 factorial experiment in a completely randomized design, so there were two factors namely depth and weight. The depth factor (D) consisted of four levels and initial weight (W) consisted of three levels, thus there were 12 treatment combination: D1W1 (25 cm, 75 g), D1W2 (25 cm, 100 g), D1W3 (25 cm, 125 g), D2W1 (75 cm, 75 g), D2W2 (75 cm, 100 g), D2W3 (75 cm, 125 g), D3W1 (125 cm, 75 g), D3W2 (125 cm, 100 g), D3W3 ( 125 cm, 125 g), D4W1 (175 cm, 75 g), D4W2 (175 cm, 100 g), and D4W3 (175 cm, 125 g). Data collected were analyzed statistically using the statistical program JMP (SAS Institute). Statistical analysis showed there was a significant differences in relative growth of seaweed K. alvarezii (P> F.0003 <.01).The best relative growth of seaweed K. alvarezzi was achieved in treatment combination D1W1 (25 cm, 75 gr) as much as 302.89%. Keywords: Seaweed, Kappaphycus alvarezii, relative growth
Efektifitas penggunaan bingkai jaring pada penjarangan benih kerang mutiara, Pinctada maxima
Authors: Tomatala Pitjont
Published: July 2019

Abstract Pearl oyster Pinctada maxima is  one of the sea resource that has an economic value in national and international market.  Recently, many companies  have succeeded in producing hundreds of  thousand spat through hatchery. However,  mortality rate of spat in rearing activityies in the sea was remain high. It was caused by unfavorable handling of spat during the spacing process. The purpose of this research was evaluate the effectiveness of using frame net in spat handling at spacing process. This research was conducted at Luv Bay of Tual City, Maluku Province on March to April 2012. The observation was conducted to measure the shell growth and survaival of  P. maxima spat. The result suggested that the use of frame net in the spacing  process of pearl oyster had great advantage. It was proven by the normal growth and high survival rate.   Keywords : Pinctada maxima, spat, growth rate, survaival rate
Pemanfaatan Jahe (Zingiber officinale Rosc) untuk Memacu Pertumbuhan Ikan Nila (Oreochromis niloticus)
Authors: Belseran Lukas , Manoppo Henky
Published: July 2019

A research had been conducted to study  the effect ginger supplemented in feed on growth of nile tilapia.  Juveniles as much as 250 individuals  (mean weight 27.31 g) were obtained from Fish Culture and Development Board  (BP3I) Tateli. The fish were placed in plastic bags and transported to Faculty of Fisheries and Marine Science.  Fish were then introduced in 15 aquaria at a density of 15 individuals per aquarium. Each aquarium was equipped with an aerator and used water recirculation system. After adaption for one weeks, fish was fed pellet supplemented with  ginger powder as treatments at five different doses including A=0, B=2,5, C=5, D=7,5, and  E=10 g/kg, each with three replications.  Fish was fed  treatment diets for four weeks at 3%/bw/day, twice daily at 08.00 am and 17.00 pm.  Growth data including absolute and relative growth were weighted at the end of feeding period. Research results showed that after four weeks of feeding, supplementation of ginger powder into fish pellet  significantly improved fish growth .  The growth of fish fed pellet supplemented with 7.5 g/kg of food was  and significantly different as compared to that of control fish.  It was concluded that supplementation of ginger powder into fish pellet potentially improved fish growth.   Keywords: Ginger, absolute growth,  relative growth, nile tilapia
Penambahan Madu Dalam Pengenceran Sperma untuk Meningkatkan Motilitas, Fertilisasi dan Daya Tetas Telur Ikan Mas (Cyprinus carpio L)
Authors: Tumanung Sudirman , Sinjal Hengky J , Watung Juliaan Ch
Published: July 2019

The research intended to determine the optimal concentration of honey addition in sperm dilution to improve sperm motility, fertilization and eggs hatchability of goldfish (Cyprinus carpio). Experimental design used was a Complete Randomized Design (CRD) with four treatments each with three replications. The concentration of honey addition used for treatment were A (0 ml of honey in 100 mL of physiological NaCl), B (0,2 mL of honey in 99.8 mL of physiological NaCl), C (0.4 mL of honey in 99.6 ml of physiological NaCl), D (0.6 mL of honey in 99.4 mL of physiological NaCl). Sperm dilution containers used was 12 plastic cups and the container fertilization and hatching eggs container used was 12 brass plates.  Spermatozoa motility observation performed immediately after dilution process. Fertilization observation was performed after 12 hours of fertilization process, and Egg Hatchability observation was performed after 72 hours of fertilization.  The observation results showed that the addition of honey in sperm dilution contributed significant effect on spermatozoa motility, fertilization and eggs hatchability of goldfish (Cyprinus carpio). This observation showed that the addition of 0.6 ml of honey in a sperm dilution was the best treatment indicated by the average percentage of spermatozoa motility (80,00%), fertilization (84,00%) and eggs hatchability (82,33%).   Keywords: Honey, spermatozoa motility, fertilization, eggs hatchability, Cyprinus carpio L
Determinasi Kandungan Kadmium (Cd) Di Perairan Pantai Malalayang Sekitar Rumah Sakit Prof Kandou Manado
Authors: Mamoribo Hulda , Rompas Robert J , Kalesaran Ockstan J
Published: July 2019

This research was conducted by purposive random sampling method with 3 points. Data were analyzed descriptively by comparing the environmental quality standard based on the Ministry of Environment No. 51. 2004. Results of laboratory analysis was the content of Cadmium (Cd) was still below the allowable threshold. Compared to Cd content of fresh water which was 0.0002 mg/L, the Cd content found in this research was lower that was 0.0001 mg/ L.   Keywords: Kadmium (Cd), Logam berat, Baku Mutu, Pantai Malalayang, RS Prof Kandou.
Daya Tetas Telur dan Sintasan Larva Dari Hasil Penambahan Madu pada Bahan Pengencer Sperma Ikan Nila (Oreochromis niloticus)
Authors: Ayer Yunus , Mudeng Joppy , Sinjal Hengky
Published: July 2019

The objective of research was to determine the concentration of honey in sperm dilution  for improving hatching rate of egg and survival of nile tilapia larvae (Oreochromis niloticus).The number of fish used was five pairs broodstock. Dilution substance was NaCl and honey.  Observations were conducted on spermatozoa motility, fertility and egg hatching rate. Experimental design used was complete randomized design. Sperm dilution substance was prepare by disolving honey  (0 mL; 0,60 mL; 0,65 mL dan 0,70 mL) in 100 mL; 99,40 mL; 99,35 mL and  99,30 mL NaCl respectively.  Each dilution was homogenized using aerator for 15 minutes. Fertilization was done 12 hours after ovulation.  Egg hatching rate was observed after ovulation. Research results showed the use of honey had significant effet on egg hatching rate, but not on larval survival.  Treatment D (0,70 mL honey in 99,30 mL NaCl) had the highest hatching rate (77.33%). Dilution ratio 1:60 was the best indicated by spermatozoa motility 96.66%, fertility 71.65, hatching rate 70% and larval survival 81.67%   Keywords : honey, egg hatching rate, larval survival, Oroechromnis niloticus
Kajian Kelayakan dan Pengembangan Lahan Budidaya Kepiting Bakau (Scylla spp) di Desa Kabupaten Minahasa Utara
Authors: M Mardiana , Mingkid Winda , Sinjal Hengky
Published: July 2019

This research intended to evaluate the area suitability of  Scylla spp at Likupang II Village, North Minahasa. The research was done descriptively by observing water quality, area availability. SWOT analysis was used to determine the development strategy.   Data were collected by interview, observation and questionnaire.  Research result showed that the potential of Likupang II Village was suitable for Scylla spp culture.  SWOT analysis showed internal factors affected the development of Scylla spp culture.  The strength were location, water quality, government support; the weakness were seed availability, opportunity, government policy and treating were season and safety.   Keywords : Scylla spp, SWOT analysis, , Likupang II, area suitability
Evaluasi Kondisi Lingkungan Akuakultur Pada DAS Tondano Di Kelurahan Ternate Baru Kota Manado
Authors: Wakman Dolvinus , Undap Suzanne L , Salindeho Indra
Published: July 2019

In this study, we evaluated the current condition of the environmental conditions of aquaculture on watershed Tondano River in terms of physical and chemical parameters. Determination of sampling points at each station is placed vertically at three predetermined points from the brigde toward the front of the aquaculture, the distance between one point to the next point was ± 50 m; whereas for the analysis of water quality parameters was done using an HORIBA water quality meters type U-536. Determination points were done by purposive sampling which refers to the physiographic location wherever possible in order to represent or describe these waters. The research was carried out for 3 weeks and was done in 3 stages, morning, afternoon and evening. For direct measurement (in situ) was performed once a week at the three points which included parameters pH, temperature, conductivity, DO, Oxidation reduction potential, turbidity, TDS, depth and GPS. Generally, the result of water quality analysis at the aquaculture still in the water quality standard PP No.82 of 2001. Keywords: Evaluation, water quality, water Lake Tondano, Aquaculture
Maskulinisasi larva ikan nila (Oreochromis niloticus) melalui penggunaan madu dengan konsentrasi berbeda
Authors: Odara Soesanti S , Watung Juliaan Ch , Sinjal Hengky J
Published: July 2019

This study aimed to determine the effect of concentration of honey on masculinization of nile tilapia female. The study had four treatments, each with three replications. The experimental design used was completely randomized design (RAL).  Data was analyse using Analysis Of Variance (ANOVA) followed by LSD at 5% and 1%.  Broodstock was  soaked in 1 L of water with treatment A 0 mL (without honey), B (5 mL honey), C (10 mL honey), D (15 mL honey) for 10 hours. After soaking, fish was reared in pan for one month. The percentage of male was determine by comparing the length and width of the red blood cells under a microscope.  The results showed the the use of honey had significant effect on female masculinization.  The percentage of male larvae tended to increase with the increase of honey dose. The highest percentage of male was obtained in treatment D (15 ml) that was 80%.   Keywords: Honey, masculinization, larvae, Oreochromis niloticus
Kejutan suhu pada penetasan telur dan sintasan hidup larva ikan lele (Clarias gariepinus)
Authors: Aer Christo V.S , Mingkid Winda M , Kalesaran Ockstan J
Published: July 2019

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.
Pengaruh Potassium Media terhadap Pertumbuhan Mutlak, Osmolaritas Hemolymp dan Energi Basal dari Pascalarva Vaname (Litopenaeus vannamei, Boone)
Authors: Kaligis Erly
Published: July 2019

Absolute Growth, hemolymp  osmolality, oxygen consumption, and basal energy of  Litopenaeus vannamei postlarvae were investigated. After post larval stage, PL-20, the postlarvae were acclimatized from sea water (25 ppt) to low salinity  water (2 ppt) over 96 h. Treatments consisted of different concentration of K+added to distilled water. Four different levels of K+ (0, 30, 60, and 90 ppm) were utilized. Ten shrimp were placed in triplicate 40-L glasses aquarium.  Results from the 42-day K+ growth trial indicated significant differences (P< 0.05) in absolute growth, hemolymp osmolality,  oxygen comsumption, and  basal energy. The values in treatment A was significantly higher than than those under the other four treatments. The results suggest treatment C were the optimum K+ level for culture  vaname postlarvae in low salinity water. Keywords :  Litopenaeus vannamei, oxygen comsumption, hemolymp
Identifikasi lingkungan perairan untuk budi daya karang hias di desa Arakan Kabupaten Minahasa Selatan
Authors: Manembu Indri S , Ngangi Edwin L.A
Published: July 2019

This research aimed to gather data and information as well as to evaluate the waters condition and water quality suitable for ornamental coral aquaculture, so the characteristic of Arakan waters for ornamental coral aquaculture could be examined.  The research applied Explorative Descriptive method with Purposive Sampling.  Research stations were established at 20 points representing the overall ecosystem of Arakan waters.  Data collected  consisted of physical conditions (bottom substrate, flow rate, turbidity, water depth, salinity, temperature, TSS) and  chemical conditions (pH, DO, nitrate, phosphate). Data were analyzed using Matching Method by matching the data obtained from sea water standard quality table for marine biota in accordance to Kep.Men. LH No. 51/2004 and data from Hutabarat and Evans (1984).  Based on physical and chemical conditions, Arakan waters was suitable for ornamental coral aquaculture or categorized as suitable. However, water temperature at the time of measurement was not proper,  thus monitoring of this parameter for longer period was required. Keywords: Arakan Village, ornamental coral, water quality, waters condition,  matching                      method
Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)
Authors: Aziz Erwin A. , Kalesaran Ockstan
Published: July 2019

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae
Penggunaan Tepung Buah Pepaya Carica papaya L Dalam Pelet Terhadap Efek Pertumbuhan Benih Ikan Nila Oreochromis niloticus
Authors: Tulung Herol A. , Lumenta Cyska , Ngangi Edwin L.A.
Published: July 2019

This study aimed to determine the effect of papaya flour at different doses on absolute  growth, daily growth, relative growth, feed efficiency, and food conversion. The treatments consisted of 0,  2.5%, 7.5%, 10% of papaya flour respectively, each with three replcations. The weight of fish were  about 18-23.58 g.  The fish were placed in aquaria with a density of  10 individual per aquarium. The experiment design is a complete randomized design. The results of analysis show that the treatments had no significant effect on growth, daily growth, relative growth, feed  efficient, and food conversion ratio. However, treatment C (7.5% papaya flour) gave the best on growth of fish, feed efficiency and food conversion ratio.
Efikasi ekstrak tanaman obat Binahong Anredera cordifolia (Ten) Steenis untuk meningkatkan pertumbuhan ikan Nila Oreochromis niloticus
Authors: Wantah Monika M. , Longdong Sammy N.J. , Kreckhoff Reni L. , Mantiri Desy M.H. , Tumbol Reiny A. , Manoppo Henky
Published: July 2019

The aim of this research was to study the effect of Binahong extract on the growth of Nile tilapia. A number of 225 fish measuring 10-12 cm in length with an average weight of 15 g was obtained from Fishery Development Center and Training, Tateli. The fish were put  in oxygenated plastic bags and transported to Laboratory of Aquaculture Technology. The fish was kept in aquarium equipped with an aerator with a density of 15 fish per aquaria. The test material used was the stem of Binahong plant stem, macerated with alcohol 70% for 3 days. The extract was then filtered with whatman filter paper and extract was concentrated using a Rotary Evaporator. The extract was further diluted to obtain  concentrations of 12.5, 25, 50, and 100 mg / mL Binahong  extract as treatment was given to the fish after mixed first into the pellet. The fish was fed with treatment feed for 30 days. Control feed was not supplemented with binahong extract. Growth data was measured at day 30 after treatment. The results showed that the extract of binahong had significant effect on fish growth (p <0.01) with the best result achieved at concentration of 100 mg/mL. In conclusion, supplementation of  binahong extract into fish feed could improve growth of  fish in which the best growth was obtained in fish fed diet containing 100 mg/mL of binahong extract.Keywords: Medicinal plants, tilapia, Anredera cordifolia, growth, maceration
Strategi pengembangan budidaya perikanan air tawar di Kecamatan Dimembe Kabupaten Minahasa Utara Provinsi Sulawesi Utara
Authors: Worang Bacthiyar C.G.S. , Sinjal Hengky J. , Monijung Revol D.
Published: July 2019

The objectives of this research were to study the profile of freshwater fish culture in Dimembe District, Analyse the prospects for the development of aquaculture in Dimembe District,  determine cultivation development strategy that is suitable with the condition and potential of Dimembe District. The method used in this research was descriptive using data in the last 5 years. Data were analysed using quantitative and qualitative descriptive analysis and SWOT analysis. Fish farmers in Dimembe District amounted to 226 people and the location of water quality supports the business of freshwater fish cultivation of indigo and carp in Dimembe District. Internal factors included business location, water quality, local government support, existance of BPBAT. Factors weaknesses were availability of less deed, and lack of human resources. External factors affecting the development of fish farming in Dimembe District consist of opportunity factor that were high fish selling value, high market share, big business opportunity, government policy. Treat factors: theft and security. Strategy steps for developing fish farming business in Dimembe District could be conducted by increasing the production of tilapia fish and goldfish, providing training on freshwater fishery cultivation and maintainng the quality of fish farming products, making agreement among farmers in maintaining the security of pond cultivation. Keywords: Dimembe District, freshwater cultivation, fish farmers, SWOT analysis
Konsentrasi Hambatan Minimum ekstrak Portulaca grandiflora terhadap penyakit Motile Aeromonad Septicaemia
Authors: Lauluw Dafid , Kreckhoff Reni L. , Longdong Sammy N.J. , Mantiri Desy M. , Watung Juliaan Ch. , Tumbol Reiny A.
Published: July 2019

The objective of this study was to determine the minimum concentration of extract in inhibiting motile motile aeromonad septicaemia. The material used was the stems and leaves of the plant P. grandiflora as much as 10 g. The crushed simplicia was then macerated with aquades with a ratio of 1:10,  then filtered with filter paper and, put in an Erlenmeyer, and evaporated in an incubator at temperature of 30°C. The sensitivity test was performed by placing a paper disc containing extract on the agar medium that had been scratched with bacterial colonies. Clear zone formed around the paper disc was compared with standard classification table for clarifying the inhibition level.  The results showed that stem and leaf extract had inhibition zone against Aeromonas hydrophila bacteria that caused motile aeromonad septicaemia. The best result was obtain at concentration of extract 3.25 mg / L) with an average diameter of 22.67 mm and for leaf extract had a moderate to strong zone for all treatments. These proved that stem and leaf extract of P. grandiflora plant had antibacterial activity and can be used as control of MAS disease caused by A. hydrophila bacteria. Keywords:  Portulaca grandiflora plant, motile aeromonad septicemia,  minimum       inbibition concentration
The potential of catappa leave to prevent Aeromonas hydrophila infection in Nile Tilapia Oreochromis niloticus
Authors: Bukasiang Selvyane , Manoppo Henky , Lantu Sartje , Bataragoa Nego E. , Lumenta Cyska , Kreckhoff Reni L.
Published: July 2019

Penelitian ini bertujuan untuk menetapkan dosis penambahan ekstrak daun ketapang dalam pakan untuk meningktkan. Ikan uji adah benih nila berukuran 5-8 cm dengan beratr ata-rata 2,93 g yang diperoleh dari Balai Perikanan Budidaya Air Tawar Tatelu. Bahan uji berupa Daun Ketapang  (Terminalia catappa) dengan dosis A= 0, B= 5 g/kg, C= 10 g/kg, D= 20 g/kg dan E= 40 g/kg  diberikan kepada ikan selama 21 hari. Ikan diberi pakan perlakuan dua kali sehari yakni pukul 09.00 am dan 16.00 pm dengan dosis pemberian 5%/berat tubuh/hari. Hasil penelitian didapatkan pertumbuhan terbaik dicapai pada kosentrasi ekstrak daun ketapang 5 g/kg.  Sebagai kesimpulan adalah ekstrak daun ketapang dapat digunakan dalam akuakultur untuk meningktkan pertumbuhan ikan.Kata kunci : Daun Ketapang, tanaman obat, ikan Nila,  A. hydrophila, budidaya ABSTRAKThe research aimed to  establish the accurate dose of catappa leave extract supplemented into feed to enhance fish growth.  The fish measuring 5-8 cm with an average weight of 2.93 g were obtained from Board of Freshwater Fisheries Aquaculture Tatelu. Catappa leave extract as much as A= 0, B= 5 g/kg, C= 10 g/kg, D= 20 g/kg dan E= 40 g/kg were added into feed and fed to fish for 21 days.  The fish wee fed twice  day at 09.00 am and 16.00 pm at 5% of body weigth a day.  Research result showed that application of 5 g of leave extract per kg of feed gave the best absolute and specific growth of fish.  Thus, supplementation of leave extract into fish feed was potential ti improve fish growth.Keywords: catappa leave, medicinal plant, Nile tilapia, aquaculture, A. hydrophila.
SOBREPESO Y OBESIDAD EN NIÑOS Y ADOLESCENTES ASEGURADOS EN UN HOSPITAL PÚBLICO. HUÁNUCO-PERÚ
Authors: Daza Loarte Dick , Meza Santiago Moisés , Gonzales Rubina Jorge , Panduro Ruiz Diana , Dámaso Mata Bernardo , Luján Pachas Marco , Tucto Berríos Joel
Published: July 2019

Introducción: El sobrepeso y la obesidad en adolescentes están experimentando, a nivel mundial, un incremento progresivo en su prevalencia, del cual nuestro país e incluso nuestra región no son ajenos. El objeto del presente estudio fue determinar la prevalencia de sobrepeso y obesidad en adolescentes de 10 a 17 años asegurados en el distrito metropolitano de Huánuco. Métodos: Se realizó un estudio observacional descriptivo-analítico transversal prospectivo, para identificar el diagnóstico de sobrepeso (IMC ≥ 85 percentil y ˂95 percentil) y obesidad (IMC ≥ 95 percentil) en adolescentes; para el cual seleccionamos al azar 387 participantes (195 del sexo masculino y 192 del sexo femenino) entre las edades de 10 y 17 años; a quienes se aplicó una encuesta estructurada con datos socio-demográficos y antropométricos. Resultados: Los resultados muestran una prevalencia global de sobrepeso de 19,6% y de obesidad de 11,6% en nuestra población adolescente; siendo la prevalencia, según género la siguiente, 19% y 15,9% de sobrepeso y obesidad en varones; y de 20,3% y 7,3% en mujeres, respectivamente (p=0,001). Dentro del grupo etario, las mayores prevalencias de sobrepeso abarcaron las edades de 10 (21,7%), 11 (19,1%), 12 (37,3%) y 13 (29,8%) años de edad; mientras que para la obesidad abarcó las edades de 10 (15,2%), 11 (21,3%) y 12 (35,3%) años de edad (p = 0,0001). Con respecto al tipo de dieta, actividad física, ingreso familiar y tipo de centro educativo; en el sobrepeso y la obesidad no se encontraron asociaciones estadísticamente significativas. Conclusiones: La población estudiada presentó una alta prevalencia de sobrepeso y obesidad, predominando éste último en los varones; con mayor tendencia en los más jóvenes.
RELACIÓN DE LOS ESTADIOS DE LA OSTEOARTROSIS TEMPOROMANDIBULAR CON LOS CAMBIOS DIMENSIONALES DEL ESPACIO ARTICULAR TEMPOROMANDIBULAR, EVALUADO CON TOMOGRAFÍA COMPUTARIZADA CONE BEAM
Authors: Espinoza Calero Karina , Santos Ortiz Nedy , Chávez Leandro Miguel
Published: July 2019

Introducción: El objetivo de este estudio es determinar la relación entre los estadios de la OA temporomandibular y los cambios dimensionales del espacio articular temporomandibular. Métodos: Se analizó los tomogramas de 120 articulaciones de 60 pacientes de 14 a 60 años atendidos en clínicas privadas, agosto 2009 / enero 2012 Lima. Con signos de artrosis degenerativa, a través de (CBCT), la ATM izquierda y derecha se evaluaron de forma independiente para cada paciente. La evaluación incluye: cambios óseos del cóndilo (erosión, aplanamiento, osteofitos, esclerosis) y de la fosa mandibular (erosión, esclerosis), clasificándolo en cada estadio de OA para luego realizar las mediciones de los espacios articulares anterior, superior y posterior de cada articulación en el plano sagital. Fueron analizados estadísticamente por el ANOVA de un factor y la prueba post hoc Bonferroni para ver la diferencia significativa de los espacios articulares entre cada estadio. Resultados: Se mostró que hubo diferencias significativas a un nivel de significancia de p<0.05 de los espacios articulares anterior (p<0.000), superior (p<0.000) y posterior (p<0.004). Siendo diferentes en el estadio inicial y terminal en los espacios articulares anterior y superior, el estadio intermedio y terminal en el espacio posterior. No mostrando diferencia en el estadio inicial e intermedio en los espacios articulares anterior y superior, y estadio intermedio y terminal en el espacio posterior. Conclusiones: El presente estudio demostró que las dimensiones de los espacios articulares (anterior, superior y posterior), comparado con parámetros normales, disminuye en cada estadio de osteoartrosis temporomandibular.
LEPRA LEPROMATOSA
Authors: Nájera Gómez Juan , Tolentino Masgo Wilinton , Gómez Hinojosa Paul , Figueroa Gamarra Luis
Published: July 2019

La lepra es una infección bacteriana crónica causada por el Mycobacterium leprae, bacilo acido-alcohol resistente obligado a vivir en el espacio intracelular preferentemente en las células de Schwann y macrófagos, que afecta la piel, los nervios y los ojos principalmente. La existencia de la patología se conoce desde la antigüedad, pero aun continúa siendo un grave problema de salud pública a nivel mundial, sobre todo en áreas endémicas como en el Perú. Su espectro clínico esta dado por la respuesta que genera el sistema inmune en contra del bacilo. Actualmente se conocen distintas formas de presentación de la enfermedad, entre ellas, los polos denominados tuberculoide y lepromatoso, la forma indeterminada y la lepra borderline. El diagnostico temprano es la principal herramienta para lograr un tratamiento adecuado, prevenir las discapacidades y rehabilitar al paciente enfermo. El tratamiento fijado por la OMS desde 1984 consiste en una terapia multimedicamentosa basada en la clasificación paucibacilar o multibacilar, según el número de lesiones cutáneas y la cantidad de bacilos presentes en la biopsia. Se reporta el caso de un paciente masculino de 50 años de edad, con diagnóstico de Lepra Lepromatosa. Se discute las pruebas diagnosticas realizadas y la terapéutica empleada.
Factores asociados a caídas en el adulto mayor en el programa de atención domiciliaria
Authors: Anaya Moya Delsi Melina , Ariza Naupay Cynthia Shirley
Published: July 2019

Introducción El objetivo del presente estudio fue determinar la asociación entre el grado de dependencia funcional, la polifarmacia y el grado de comorbilidad con las caídas del adulto mayor. Métodos: Se realizó un estudio analítico de casos y controles que incluyó 300 pacientes pertenecientes al Programa de Atención Domiciliaria - EsSalud Huánuco. El método de muestreo fue no probabilístico por conveniencia. Se realizó entrevistas obteniéndose información sobre datos sociodemográficos (edad, sexo), variables antropométricas (talla y peso) factores de riesgo de caídas intrínsecos [polifarmacia; grado de dependencia funcional (evaluada con índice de Barthel) y grado de comorbilidad (evaluado con el índice de Charlson)]. Resultados: Se incluyeron 300 pacientes, de los cuales 169 pacientes sufrieron caída. Se encontró mayor predominio de mujeres (64% del total). Se encontró asociación entre la variable caída con las variables Grado de dependencia funcional (OR= 1,71; p= 0,0386), polifarmacia (OR= 2,10; p= 0,0023) y grado de comorbilidad (OR=1,85; p<0,02). No se encontró asociación con la variable sexo (p=0,098) ni Edad (p=0,40). Conclusiones: La polifarmacia, el grado de dependencia y el grado de comorbilidad fueron factores asociados a las caídas en el adulto mayor.
Factores relacionados a cáncer gástrico en un hospital público de Huánuco
Authors: Eulogio Valenzuela Frida Katerina , Narciso Criollo Rodis Augusto
Published: July 2019

Introducción El objetivo del presente estudio fue determinar la relación entre infección por Helicobacter pylori, nivel socio económico y factores dietéticos con cáncer gástrico en pacientes del Hospital Regional Hermilio Valdizán - Huánuco, durante el periodo 2009 - 2014. Métodos: Estudio observacional de Casos y Controles. Se aplicó el instrumento de recolección de datos inicialmente a las historias clínicas y para completar la información se ubicó a los pacientes y/o familiares. Además, se obtuvo muestras séricas para determinar la infección por Helicobacter pylori mediante método ELISA IgG. El análisis estadístico se realizó con el programa estadístico SPSS 15.0 y EPIDATA. Resultados: Se encontraron 135 casos con diagnóstico endoscópico de cáncer gástrico y 135 controles ingresados aleatoriamente (N= 270), 140 (51.9%) pacientes fueron varones. 188 (69.6%) pacientes presentaron pruebas positivas mediante Elisa IgG para Helicobacter pylori. Se encontró asociación estadísticamente significativa entre cáncer gástrico e infección por Helicobacter pylori, también se encontró asociación significativa con el sexo masculino, con la edad mayor a 60 años y con añadir sal a las comidas. Con respecto al nivel socio económico, antecedentes familiares, lugar de procedencia y dieta no se encontró asociación significativa con cáncer gástrico. Conclusiones: El presentar infección por Helicobacter pylori, ser del sexo masculino, edad mayor a 60 años y con añadir sal a las comidas fueron factores de riesgo para desarrollar cáncer gástrico. El nivel socio económico, los antecedentes familiares, el lugar de procedencia y la dieta no fueron factores de riesgo para cáncer gástrico.
Litiasis biliar o colecistectomía asociada a síndrome metabólico en pacientes del Hospital II - EsSalud, Huánuco
Authors: Fierro Barzola William , Mariano Ureta Gianmarco Saúl , Curo Niquén Jimmy
Published: July 2019

Introducción: El objetivo del presente estudio fue determinar la relación entre litiasis biliar o colecistectomía con síndrome metabólico en pacientes del hospital II - Essalud, Huánuco 2014. Métodos: Estudio de casos y controles en 180 personas con síndrome metabólico (casos) y 180 que no presentaban síndrome metabólico (controles). Se realizó una encuesta en la que se le preguntaba a las personas su edad, género, tipo de dieta, antecedentes patológicos familiares, síndrome metabólico y colecistectomía. Resultados: De los 360 sujetos, respecto a colecistectomía, se encontró que 162 (51.3%) no fueron colecistectomizados y no presentaban síndrome metabólico; 154 (48.7%) colecistectomizados sí presentaron síndrome metabólico, no se encontró una relación significativa, pero sí se le puede atribuir calidad de riesgo para hacer síndrome; sin embargo, la litiasis biliar sí se constituye en un factor de riesgo. El Género masculino fue el que presentó mayor número de casos de síndrome metabólico, estos fueron 111 (52.1%), por otro lado, fueron los mayores de 35 años 139 (47.1%) los que tenían síndrome metabólico. Al considerar la dieta se encuentra una relación significativa (p= 0), pero en función del nivel de consumo de grasas. Conclusiones: No hay una relación significativa entre colecistectomía o litiasis biliar con el síndrome metabólico.
Factores de riesgo para la infección del tracto urinario en gestantes del Hospital Hermilio Valdizán de Huánuco
Authors: Randhu Cuba Percy Agüero , Figueredo Domingo Edwin Julio , Dámaso Mata Bernardo
Published: July 2019

Introducción: El objetivo del presente estudio fue identificar si la primigestación, la adolescencia y la anemia son factores de riesgo para la infección del tracto urinario. Métodos: El estudio analizó los datos de pacientes atendidas desde enero a diciembre del 2010, se desarrolló un estudio observacional, analítico, transversal, retrospectivo de casos y controles de 130 pacientes: 65 casos y 65 controles del servicio de Gineco-Obstetricia las cuales fueron elegidos al azar del registro del mencionado servicio. Los casos fueron gestantes con diagnóstico de infección urinaria y los controles fueron gestantes que acudieron durante ese periodo por diversos motivos sin diagnóstico de infección urinaria. La recolección de la información consistió en revisar las historias clínicas tomando como punto de referencia la hoja de control prenatal. Las características basales de los grupos se compararon mediante la prueba de Chi cuadrado. Se estimó la asociación entre los dos grupos y los factores de riesgo por medio del odds ratio (OR), también, se estimó asociaciones bivariadas utilizando la prueba T. Resultados: Las gestantes adolescentes de 11 a 19 años tienen más riesgo que las gestantes de 20 años o mayor de presentar infección del tracto urinario (OR: 2,81) (IC 95%: 1,20- 6,56), la presencia de anemia también se asoció a mayor riesgo de presentar infección del tracto urinario (OR: 2,72) (IC 95%: 1,08-6,83), así como la primigestación (OR: 3) (IC: 95%: 1,45-6,20). Conclusiones: La infección del tracto urinario en el embarazo fue asociado a la primigestacion, adolescencia y la presencia de anemia.