bluuebunny/crossref_metadata_2025 huggingface dataset:
On the issue of evidence admissibility in civil proceedings
Authors: Nazarov Valentin V. , Zolotuhin Aleksandr D.
Published: April 2021

We investigate the procuring evidence in civil proceedings, we come to the conclusion that the court in the procuring evidence is the determining subject. This conclusion follows from the content of the rules of the court’s activity to determine the subject of evidence. By determining the subject of evidence, the court thereby determines and organizes the activities of the persons participating in the case, to prove the circumstances to which it indicated in the subject of evidence, regardless of the persons’ opinion par-ticipating in the case regarding the circumstances that they asked to be in-cluded in the subject of evidence, but the court did not. The decisive role of the court in the procuring evidence is also confirmed by the legislator’s right to apply the rules of evidence admissibility presented by the persons participating in the case for examination in the court session, as well as the right to offer the persons participating in the case to submit additional evidence. We conclude that the rules applied by the court to determine the evidence admissibility represent a specific set. This set includes, in addition to the general rule for determining the evidence admissibility, the rule according to which the evidence admissibility is established by determining their relevance and sufficiency to the circumstances of the subject of evidence. The set also includes rules for determining the procedural form of the evidence presented, including the rules for obtaining them. We believe that the legislator should exclude the current rule on the violations recognition of the procedural form of obtaining evidence as formal, as not complying with the requirements of the legality principle.
Legal and organizational basis for ensuring road safety in the Ukrainian SSR in 1948–1953
Authors: Tishakov Maksim P.
Published: April 2021

The work, based on previously little available for research, as well as materials and documents found in archival institutions, reflects the legal basis for ensuring road safety in 1948-1953, the state and organizational and legal measures taken in the field of combating accidents in road transport at the republican level by the example Ukrainian Soviet Socialist Republic. Attention is focused on the key problems that determine the development of the road safety system, the measures taken, their effectiveness, mistakes and achievements. Measures to counteract accidents in vehicles are investigated from a historical and legal standpoint, a critical and detailed analysis of decrees and orders of the government, departmental regulatory legal acts. It was found that the presence of a significant number of administrative decisions of the republican authorities of Soviet Ukraine, although it was a rather progressive step for its time, did not fully take into account the reality of achieving the set goals, local conditions and peculiarities. At the same time, the functioning of the emerging road safety system was significantly hampered by the lack of a unified national policy in the context of the rapid growth and development of the country’s automobile and road complex.
O semanticocentrismo de Ronald Langacker
Authors: Silva Gustavo Augusto Fonseca
Published: April 2021

Para Ray Jackendoff, os modelos linguísticos desenvolvidos por Noam Chomsky distorcem a natureza da linguagem devido a seu “sintaticocentrismo”. Por esse termo, Jackendoff entende a premissa adotada por Chomsky desde o modelo padrão da gramática gerativa (Aspectos da teoria da sintaxe) até o Programa Minimalista de que o componente sintático é proeminente em relação aos componentes fonológico e semântico, que seriam tão somente interpretativos. O próprio Jackendoff, no entanto, também observa que desde ao menos os anos 1970 muitos pesquisadores cometeram o erro oposto ao de Chomsky por negar à sintaxe um papel gramatical relevante. Considerando-se esse quadro, analisa-se neste artigo a gramática cognitiva de Ronald Langacker, na qual a sintaxe é substituída pela semântica como componente linguístico central. O objetivo é mostrar como Langacker contrapôs o sintaticocentrismo chomskiano com uma espécie de “semanticocentrismo”. Ao fazê-lo, conforme se detalha neste trabalho, acabou por também deturpar gravemente a constituição da linguagem.
Sentenças absolutas em português brasileiro adulto e infantil
Authors: Rezende Camilla de , Grolla Elaine
Published: April 2021

As sentenças absolutas consistem em uma alternância na valência de verbos transitivos encontrada no Português Brasileiro (PB), tal como “A casa vendeu”. Segundo Negrão & Viotti (2010), nas absolutas, o argumento que representaria a energia responsável pela causa do evento não está presente e nem chega a ser conceitualizado. Não há, portanto, o que chamam de uma força indutora (NEGRÃO & VIOTTI, 2010). As autoras defendem que, nas absolutas, vP não é projetado. Hipotetizamos ainda que verbos instrumentais (como “pintar”) podem ter a força indutora conceitualizada no instrumento (pincel). Para testar as hipóteses, conduzimos um experimento de produção eliciada com 56 crianças entre 3;8 e 6;0 (divididas em dois grupos) e 28 adultos falantes de PB. Os participantes assistiram a animações e tinham que dizer a um fantoche o que havia acontecido em cada uma delas. As variáveis independentes foram: presença de agente (com e sem) e tipo de verbo (instrumental e não instrumental). Os resultados sugerem que ambas as variáveis influenciaram as respostas tanto de crianças como de adultos: os contextos sem agente (p-valor <.0001) e os contextos com verbos não instrumentais (p-valor <.0001) se mostraram mais propícios para a produção de sentenças absolutas. Com relação à diferença de comportamento entre os grupos, as crianças mais novas testadas produziram mais frequentemente absolutas do que passivas, o que indica que a absoluta, por ser menos complexa, é uma estrutura mais acessível nos estágios iniciais da aquisição do PB.
sobrevida fascista nas formas urbanas contemporâneas
Authors: Valente Rodolfo de Almeida
Published: April 2021

O número crescente de países governados por figuras de extrema-direita suscitou a retomada do debate sobre o fascismo e a possibilidade de sua reedição histórica. Neste ensaio, procura-se deslocar o eixo de análise às relações sociais cotidianas, suas formas urbanas e, em particular, à denominada forma de vida em condomínio edilício, tendencialmente hegemônica nos grandes centros urbanos. Dessa perspectiva, a questão sobre a sobrevivência do elemento fascista na contemporaneidade é centrada, não na possibilidade de os regimes políticos atuais transmutarem-se em regime fascista, e sim na sua (re)produção no âmbito da erosão da experiência aprofundada pelo dispositivo totalitário das grandes metrópoles da era neoliberal.
Situación actual de la interpretación sanitaria en la isla de Gran Canaria
Authors: Pérez-Luzardo Díaz Jessica , Santana García Mónica del Carmen
Published: April 2021

El objetivo principal de este estudio es averiguar los problemas de comunicación que existen entre el personal sanitario y el paciente extranjero en los centros sanitarios de Gran Canaria. Tras enmarcar la interpretación sanitaria dentro de la interpretación bilateral y, por consiguiente, dentro de la interpretación en los Servicios Públicos, se define la interpretación sanitaria y se aborda la situación de esta en España y, en concreto, en Canarias, que difiere la presencia de la profesión en otros países. Se explica un estudio observacional descriptivo realizado mediante encuestas entre el personal sanitario y no sanitario (administrativos y celadores) de los complejos hospitalarios públicos de Gran Canaria. Se analizan los resultados obtenidos, que ponen de manifiesto la dimensión del problema de comunicación que existe en los diferentes servicios de los complejos hospitalarios en estudio y las soluciones ad hoc que se aplican en la mayoría de los casos a cargo del propio personal sanitario, así como la ausencia casi total de profesionales de la interpretación.
Meaning construction in the Brexit process
Authors: Cortés de los Ríos María Enriqueta , Hernández Aparicio Tamara
Published: April 2021

El objetivo de este estudio es arrojar algo de luz sobre la forma en que se construye el significado en diferentes etapas del proceso del Brexit a través de algunas portadas de la revista The Economist. El presente artículo se encuentra dentro del ámbito de la investigación sobre metáforas, metonimia y esquemas de imágenes. Este análisis se ha estructurado de acuerdo con la Teoría de la Metáfora Conceptual (Lakoff y Johnson 1980; Ruiz de Mendoza y Pérez, 2011, entre otros) por un lado, y la Teoría Multimodal (Forceville 1996, 2009, 2012, 2016; Forceville y Urios -Aparisi, 2009) por otro, que han revelado que el significado se crea a través de modos de comunicación distintos de los verbales. El artículo muestra el papel de la metáfora y la metonimia monomodal y multimodal como mecanismos persuasivos en portadas de revistas y la interacción de éstas con esquemas de imagen. Además, los resultados revelan que muchas metáforas multimodales están basadas en metonimias.
L'enseignement-apprentissage du français juridique en contexte francophone: Approche contextuelle et interculturelle
Authors: El Krirh Khatima
Published: April 2021

En este artículo damos cuenta de la incidencia del contexto jurídico y bilingüe en la enseñanza-aprendizaje del francés jurídico (FOS). Así, fenómenos como la diglosia, bilingüismo y bijuridismo repercuten en la configuración de un sistema jurídico. Para ello, se toma como ejemplo Marruecos como país del Magreb francófono cuyo sistema jurídico está caracterizado tanto por una dualidad jurídica (derecho islámico y derecho francés) como lingüística (árabe y francés). El análisis del corpus representativo revela que las asimetrías conceptuales entre dos sistemas jurídicos expresados en lengua francesa solo se pueden enseñar y aprender aplicando un enfoque contextual e intercultural.
Relación de los indicadores económicos, sociodemográficos, de salud y de desarrollo social con el curso de la mortalidad por COVID-19 en los primeros 120 días de pandemia
Authors: Ferrándiz Espadin Renato , Cieza Zevallos Javier
Published: April 2021

Objetivo: Describir y analizar la mortalidad por la Covid-19 en función de aspectos sociales: indicadores económicos, sociodemográficos, de salud y de desarrollo social, de los primeros países afectados al inicio de la pandemia. Material y métodos: Estudio longitudinal descriptivo y analítico del modelo de casos. Se escogió en total 63 países afectados en diferentes magnitudes y seguidos diariamente desde su primer caso declarado hasta el día 31 de mayo del año 2020 con datos de la OMS, PAHO, KNOEMA, DATOS MACRO, INDEX MUNDI y la fuente de datos de la Universidad de Johns Hopkins. Resultados: En la mortalidad temprana y a los 60 días, la variable más relevante fue el día promedio que se confirmó el primer caso. A los 75 días la variable más fuertemente asociada fue la obesidad de adultos. Conclusiones: Se aprecia que sociedades con mayor esperanza de vida y donde prevalecen personas con mayores tasas de obesidad y enfermedades pulmonares crónicas tuvieron un riesgo de mortalidad significativamente mayor al inicio de la pandemia con respecto a países donde predominan las enfermedades transmisibles y, que a pesar de que un país cuente con abundantes recursos económicos, existen elementos que inevitablemente conllevan a desenlaces fatales.
Malnutrición materno-fetal: Revisión de la bibliografía internacional y la urgencia de estudios, prevención e intervención en el Perú
Authors: Uceda Jorge E. , Caravedo-Reyes Luís , Figueroa Maria Luísa
Published: April 2021

En el Perú, la malnutrición materno-fetal ocurre con frecuencia, pero su prevención, intervención y seguimiento no ha sido investigada en relación al conocimiento y experiencia de países emergentes. Los autores presentan una revisión actualizada de los avances científicos sobre malnutrición materno-fetal, con el fin de prevenir y manejar un empobrecido capital humano, que constituye nuestro principal y más urgente problema sanitario.
Relación entre la gestión de calidad total y el desempeño exportador de Mypes exportadoras peruanas de cacao en grano 2020
Authors:   , Juárez Sánchez Johana Evanell
Published: April 2021

El objetivo de la presente investigación fue sondear la relación entre la gestión de calidad total y el desempeño exportador de las empresas exportadoras peruanas MYPE en el Perú para el año 2020. De esta forma, se buscó contribuir a las empresas brindándoles información que puede ser de apoyo para el proceso de mejora de su producto de exportación logrando obtener una mayor cantidad de exportaciones generando estrategias de éxito a largo plazo para que el cliente y el empleado estén satisfechos llegando a tener una calidad de producto en todas sus etapas produciendo innovaciones continuas para alcanzar y posicionarse en el mercado internacional. Por otro lado, el método que se utilizo fue el de análisis cuantitativo con un tipo de investigación correlacional y un diseño no experimental transversal, con una muestra de 50 empresas exportadoras de cacao en grano en el Perú. El instrumento de medición que se usó fue cuestionario, para mediar las variables gestión de calidad total y desempeño exportador se utilizó el cuestionario de los autores Imran, Hamid y Aziz (2018). Los resultados alcanzados en la presente investigación validaron que la gestión de calidad total si se relaciona con el desempeño exportador de las empresas MYPES exportadoras peruanas de cacao en grano 2020.
Strategija razvoja turističke destinacije
Authors: Magaš Dragan
Published: April 2021

Bez identifikacije, registracije i valorizacije turističkih atrakcija, a potom i cjelokupnih turističkih uvjeta ne može biti racionalnog planiranja razvoja turizma u skladu s okolinom. Osnovna pretpostavka ostvarenja postavljenih ciljeva je svakako strategija razvoja izrađena na svim razinama (lokalnom, regionalnom i nacionalnom). U tom smislu, u radu se stavlja težište na: - kriterije za moguće definiranje turističke destinacije, i u skladu s tim - na način izrade i provođenje strategija razvoja turističkih destinacija, kao preduvjeta za operacionalizaciju postavljenih ciljeva strategije razvoja hrvatskog turizma na nacionalnoj razini.
Polyhedral communication in architecture
Authors: Butragueño Díaz-Guerra Belén , Raposo Grau Javier Fco. , Salgado de la Rosa María Asunción
Published: April 2021

There are multiple communicative strategies in architecture depending on the target audience. The mechanisms of representation may radically vary when the recipient is the client, the constructor, the curator, a colleague or even an instructor (1). As an example, the approach may be mainly visual when the target is the client. However, it may be strictly structural to start a conversation with a peer ad mostly conceptual when pointing to the jury members in a competition. In certain cases, the adaptation of the graphic language to the different scenarios leads to such a distortion on the message that the original project is hardly recognizable. To illustrate this point, the article will focus on the project of the Public Seattle Library, designed by the architect Rem Koolhaas. The analysis of five different communicative strategies of the project show peculiar and unequal graphic narrations: from the concept book of the competition, the website of LMN (the American partners of Rem Koolhaas- OMA in this particular project), the website of OMA, the coverage on the Seattle press, the Library Brochure for teenagers and the approach developed on the "Content" book. Rem Koolhaas coined the concept of "design of information" to express the fundamental relationship between the message and media in architecture. In his practice, the communicative strategy is present from the beginning of the design process till the final stages. In this article, the thoughtful comparison of these examples will enable to extract conclusions on the use of communication in architectural design and reflect on the iconic and communicative dimension of architecture in general.
La Gestión del Duelo y su Influencia en la Educación Universitaria durante la Pandemia del COVID 19 = The Mourning Management and its Influence on University Education during the COVID 19 Pandemic
Authors: Recalde Esnoz Irantzu , Ferrández Vega Daniel , Marcos Sánchez Rafael
Published: April 2021

La pandemia ocasionada por el coronavirus ha provocado una crisis sin precedentes en todos los ámbitos nacionales. El sistema educativo se ha visto envuelto en un cambio nunca antes visto que ha obligado a una transformación digital acelerada, suponiendo una modificación en el modelo de enseñanza-aprendizaje al que estábamos acostumbrados. Sin embargo, en paralelo a esta transformación una gran parte del estudiantado se ha visto afectada por la enfermedad provocada por el SARS-CoV-2 e incluso han sufrido el fallecimiento de algún familiar o persona cercana.En este trabajo se pretende realizar un acercamiento a la situación real que están viviendo los estudiantes de la Escuela Técnica Superior de Edificación durante la crisis sanitaria. Para ello, con ayuda de la técnica de la encuesta online, se ha consultado al alumnado acerca de su estado emocional actual y sus emociones ante el gran número de fallecimientos que cada día se retrasmiten en los medios de comunicación. Los resultados muestran como un elevado número de participantes, el 25.5%, ha sufrido el fallecimiento de algún familiar o allegado durante este tiempo de pandemia. Esto nos hace reflexionar sobre cómo está sobrellevando el sistema educativo universitario estas difíciles situaciones, si realmente se conocen y si se deberían poner más medios para mejorar el bienestar emocional del estudiantado.AbstractThe pandemic caused by the coronavirus has caused an unprecedented crisis at all national levels. The education system has been involved in a change never seen before that has forced an accelerated digital transformation, assuming a modification in the teaching-learning model to which we were used. However, in parallel to this transformation, a large part of the student body has been affected by the disease caused by SARS-CoV-2 and has even suffered the death of a relative or close person.This work aims to make an approach to the real situation that students of the Higher Technical Building School are experiencing during the health crisis. To do this, with the help of the online survey technique, the students were consulted about their current emotional state and their emotions in the face of the large number of deaths that are broadcast in the media every day. The results show how a high number of participants, 25.5%, have suffered the death of a relative or close friend during this time of the pandemic. This makes us reflect on how the university educational system is coping with these difficult situations, if they are really known and if more means should be put in place to improve the emotional well-being of the student body
Effects of overliming on the nutritional status of grapevines with special reference to micronutrient content
Authors: Olego Miguel A. , Quiroga Miguel Javier , Sánchez-García Mario , Cuesta Mateo , Cara-Jiménez Jorge , Garzón-Jimeno José Enrique
Published: April 2021

Aluminium plays a central role in soil acidity, which is one of the main constraints on grape production in humid, northern temperate viticultural regions. To decrease the acidity of vineyard soil, it is usually amended with alkaline materials that provide conjugate bases to weak acids (liming). However, one practical consideration is the danger of overliming, which has potential implications in terms of yield reduction and decreased bioavailability of several mineral nutrients. The main aim of this study was to evaluate the effects of overliming using dolomitic lime on grapevines growing on acid soil. The effects on the topsoil fertility parameters (0–30 cm), petiole and berry nutrient levels, berry weight and must quality properties were studied in a vineyard planted with Vitis vinifera L. cv. Mencía for three years (2014–2016). Data analysis performed using a mixed model that took into account both random effects (year of sampling) and fixed effects (liming treatments) showed that overliming decreased the manganese content in both leaf and berry tissues. Until now, nothing was known about the effects of overliming on both vine nutritional status and harvest quality properties, thus this study fills an important knowledge gap.
Efficacy Bacteriophage in Removal Pseudomonas aeruginosa from Infectious Surfaces
Authors: Rahimzadeh Golnar , Rezai Mohammad Sadegh
Published: April 2021

Nosocomial infections can be transmitted by contaminated hospital surfaces with resistant pathogens. conventional sanitations are not efficiently contributing to removing resistant pathogens. Bacteriophages suggest as decontaminating agents, safe, their selective ability to kill specific bacteria. This work aimed to assess the efficiency of a phage in removing Pseudomonas aeruginosa from different hard surfaces. The decontamination ability of phages w was tested in vitro against Pseudomonas aeruginosa strain. Cystoviridae Phages with titer (2 × 1012 PFU/mL) can efficiently reduce viable bacterial cells on contaminated surfaces. The treated surfaces with alcohol 70% and phage showed an evident drop of bacterial cell number from 1 h to 24 h. These results suggest that bacteriophages are biocontrol agents removing nosocomial infection pathogens transmitted by contaminated surfaces in the hospital environment.
Development and Testing of a Method for Detecting Lujo Virus RNA by Reverse Transcription and Real Time Polymerase Chain Reaction
Authors: Naidenova E. V. , Dedkov V. G. , Agafonov D. A. , Senichkina A. M. , Safonova M. V. , Kutyrev V. V.
Published: April 2021

The aim of the study was to develop and assess the efficacy of a method for Lujo virus RNA detection in clinical and biological samples using one-step real-time RT-PCR.

Materials and methods. In order to select the conservative regions of the genome, we utilized the available in GenBank database Lujo virus sequences (https://www.ncbi. nlm.nih.gov/genbank) aligned in BioEdit 7.2.5 software package ( (IbisBiosciences, USA). To conduct one-round RTPCR, reverse transcriptase and TaqF-polimerase were used. Recombinant Escherichia coli strain, XL1-Blue, containing pGEM-T plasmid with inserted synthetically-generated fragment of the virus genome, was produced to make positive control sample (PCS). Constructed recombinant plasmids were used for creating RNA-containing PCS with protective protein shell of MS2-phage. Determination of specificity of the developed method was performed with the help of control panel of RNA and DNA of 23 viral strains related to 10 families; the sensitivity – the panel of biological samples artificially contaminated with PCS. Further testing was carried out at the premises of laboratory of the Russian-Guinean Center for Epidemiology and Prevention of Infectious Diseases (Kindia, Republic of Guinea) on 265 blood sera from practically healthy persons, 110 blood sera of cattle, 83 suspensions of ticks, and 165 suspensions of organs of small mammals collected in the territory of Guinea.

Results and discussion. Two conservative polymerase gene fragments have been chosen as targets for Lujo virus RNA detection using RT-PCR. The combination of primers and probes has been experimentally selected, optimum composition of reaction mixture for PCR and mode of RT-PCR set-up established, as well as control samples C+, internal control, positive control sample developed. Sensitivity of the proposed method is 5·103  GE /ml, specificity – 100 %.
Role of Ixodes Pavlovskyi (Acari, Ixodidae) in Borreliosis Epizootic Process at the Island Russky
Authors: Nikitin A. Ya. , Sabitova Yu. V. , Rar V. A. , Morozov I. М. , Gordeiko N. S. , Allenov A. V. , Kaverina G. B. , Babkin I. V. , Tikunova N. V. , Andaev E. I.
Published: April 2021

The incidence of Ixodes tick-borne borreliosis (ITBB) at the Island Russky (Primorsk Territory) is higher than at the mainland. There is a bidominant structure of the tick population with mass occurrence of Ixodes persulcatus and I. pavlovskyi at the island. Due to the higher infectivity and aggressiveness of I. persulcatus, it is suggested that it plays a key role in high activity of the ITBB focus at the island.

The aim of the study: to characterize the role of I. pavlovskyi in the borreliosis epizootic process based on the study of the structure of the borrelia genotype complex isolated from Ixodes ticks at the Island Russky.

Materials and methods. The ticks were collected from vegetation at three sites. Ixodide species were determined morphologically and using PCR for mitochondrial gene cox1 and nuclear multicopy locus ITS2. Infection of 233 ticks was analyzed with the species identification confirmed by all methods. Borrelia genospecies were determined by multiplex two-round PCR with the primers specific to the gene fragments 5S and 23S rRNA of Borrelia burgdorferi sensu lato complex.

Results and discussion. Coexistence of I. persulcatus and I. pavlovskyi at the island was confirmed, with different ratios in space and a gradual increase in the proportion of the latter species. The circulation of three etiological agents of ITBB: Borrelia garinii (predominantly in I. pavlovskyi), B. bavariensis and B. afzelii (in I. persulcatus) was identified. Thus, an active natural focus of ITBB has formed at the island, probably including two uninsulated chains of borrelia circulation. One chain combines I. persulcatus and small and large mammals as the tick feeders. The second – I. pavlovskyi that can feed on mammals and birds. Due to the spatial variability of the ixodide population structure at different sections of the island, the ratio of the circulating borrelia species can change, and this species becomes the main vector of the pathogenic agents in areas of I. pavlovskyi absolute dominance.
Infection Rate in the Population of Ticks Hyalomma marginatum in the Territory of the Crimean Hemorrhagic Fever (CHF) Natural Focus and Assessment of the Link with the CHF Incidence in the Stavropol Region
Authors: Tsapko N. V. , Dubyansky V. M. , Gazieva A. Yu. , Ashibokov U. M. , Volynkina A. S.
Published: April 2021

Objective of the study was to assess the infection rate in the population of the main CCHF virus vector Hyalomma marginatum and its impact on the CHF incidence in the Stavropol Region.

Materials and methods. The data on spontaneous infection of H. marginatum ticks in the territory of the CHF natural focus in the Stavropol Region for the period 2012–2018 are presented. In total, more than 22.000 H. marginatum specimens pooled in 1546 samples were tested. The indication of CCHF virus was performed by PCR method using the reagent set for detecting RNA of the CCHF virus “AmplySens® CCHFV-FL” (produced by Central Research Institute of Epidemiology, Russia). To determine the individual infection rate in pools Beklemishev`s method was used. The data on CCHF incidence and amount of tick-bitten persons are taken from official statistics and Annual Epidemiological Report on CHF Incidence provided by the Rospotrebnadzor Administration in the Stavropol Region for the period of 2012–2018.

Results and discussion. RNA of CCHF virus was detected in 161 (10.4 %) out of 1546 pooled ticks. The highest infection rate (20 %) was among nymphs. The infection rate for pooled males of H. marginatum was 11 % and for females – 8.5 %. Circulation of Crimean-Congo hemorrhagic fever virus is confirmed in major part of the Stavropol Region. The average infection rate among H. marginatum population was 1.54 % for the period of 2012–2018, ranging from 0.23 % in 2014 to 2.97 % in 2017. It is established that the level of infection rate among H. marginatum population does not affect the CHF incidence in the Stavropol Region. Probably it is not the number of infected ticks in the population that determines the CHF incidence rate but their abundance.
Features of the Biological Properties of Vibrio cholerae Isolated during the Monitoring of Water Bodies in Rostov-on-Don from 1989 to 2018
Authors: Ezhova M. I. , Levchenkо D. А. , Arkhangelskaya I. V. , Kruglikov V. D. , Nepomnyashchaya N. B.
Published: April 2021

The goal is to study the features of the biological properties of Vibrio cholerae strains found in environmental objects (EO) on the territory of Rostov-on-Don from 1989 to 2018.

Materials and methods. Bacteriological, molecular biological and statistical methods were used to study 73 strains of cholera vibrios O1, O139 and 1702 strains of nonO1/nonO139 serogroups isolated over a 30-year period.

Results and discussion. It was found that in Rostovon-Don, 78.0 % of toxigenic strains were isolated from all isolated from environmental protection in Russia, among which 2 were genetically unchanged. The V. cholerae O1 classical strain and 72 V. cholerae El Tor strains were found, of which 70 % belonged to the Ogawa serovar, and 3 % of the nontoxigenic strains were susceptible to the classical phage. A “very weak direct relationship” was statistically revealed between the isolation of V. cholerae O1, O139 and V. cholerae nonO1/nonO139 strains, in which the dominant serogroups (O67, O76, O75, O53, O16) were identified, and 18 % of the strains showed atypical agglutinability. Revealing the biological characteristics of V. cholerae strains contributes to the optimization of monitoring studies.
Analysis of Epidemiological Situation on Crimean Hemorrhagic Fever in the Russian Federation in 2020 and Prognosis for 2021
Authors: Volynkina A. S. , Maletskaya O. V. , Skudareva O. N. , Tishchenko I. V. , Vasilenko E. I. , Lisitskaya Ya. V. , Shaposhnikova L. I. , Kolosov A. V. , Rostovtseva D. V. , Vasilenko N. F. , Dubyansky V. M. , Prislegina D. A. , Yatsmenko E. V. , Kulichenko A. N.
Published: April 2021

The review presents an analysis of the epidemiological and epizootiological situation on Crimean hemorrhagic fever in the Russian Federation in 2020. During the stated period, 32 CHF cases were registered, which is the minimum indicator since the activation of the natural focus of CHF in 1999. The mortality rate was 3.1 %. The incidence was recorded in the Rostov, Astrakhan Regions, Stavropol Territory, the Republics of Dagestan and Kalmykia. A significant decrease in the incidence of CHF was noted in all entities of the Southern Federal District and the North-Caucasian Federal District. An imported from the Republic of Crimea case of CHF was detected in Moscow. The seasonality of morbidity, occupational, and age composition of CHF patients, modes of transmission, features of the clinical course of the disease in 2020 corresponded to the data of long-term observations. Epizootiological monitoring of the territory of the CHF natural focus showed that the abundance of Hyalomma marginatum adults and the percentage of Ixodidae tick pools positive for the presence of CCHF virus markers corresponded to the average long-term indicators at stationary observation points in 2020 , which indicates the persisting epizootiological disadvantage of the territory of the natural CHF focus in the Russian Federation. The persisting high numbers and infection rate of H. marginatum ticks can contribute to the development of an unfavorable epidemiological situation in the south of the country with a possible increase in the incidence of CHF in the Russian Federation in 2021.
Peculiarities of Epidemiological Situation on the West Nile Fever in the Russian Federation in 2020 and Forecast for Its Development in 2021
Authors: Putintseva E. V. , Udovichenko S. K. , Boroday N. V. , Nikitin D. N. , Baturin A. А. , Molchanova E. V. , Shpak I. M. , Fomina V. K. , Nesgovorova A. V. , Antonov A. S. , Prilepskaya D. R. , Viktorov D. V. , Toporkov A. V.
Published: April 2021

The paper presents an analysis of West Nile Fever incidence in the Russian Federation in 2020, summarizes the results and identifies problematic issues of the pathogen monitoring. Manifestations of West Nile Fever in 2020 were characterized by a low incidence rate (10 times lower than the average long-term value) with sporadic cases registration in the endemic areas of the Southern (9 cases) and Central (1 case) Federal Districts. A discrepancy between the morbidity structure (distribution by age, sex, social status) and the trends that have developed in Russia in recent years is shown. The analysis of officially recorded cases doesn’t characterize the epidemic process of West Nile fever in Russia during 2020-season as a whole. The generalized results of monitoring of the West Nile virus circulation in environmental objects in 2020 indicate a decrease in the effectiveness of its implementation and a low detectability of pathogen markers. A decrease in the volume of diagnostic studies for the active detection of patients with West Nile fever in the epidemic season (5.7 times lower compared to 2019), as well as serological screening of healthy population samples (1.6 times) has been established. The results of a molecular-genetic study of the pathogen showed that lineage 2 of the pathogen was circulating in the European part of Russia. The circulation of the lineage 4 of the virus in the enzootic cycle in the Republic of Kalmykia was found out. The genome sequences of 11 West Nile virus isolates allocated in 2019 and 2020 were obtained. Phylogenetic analysis revealed that all isolates allocated in the Volgograd Region and isolates from the Rostov and Astrakhan regions belong to the Volgograd clade of the lineage 2 of the West Nile virus. Based on the assessment of abiotic and biotic factors, possible local increases in the incidence of West Nile fever in 2021 in the regions of the Southern and North Caucasian Federal Districts and in the south of Western Siberia have been substantiated.
Digital metering hands control to customers
Authors: Nitschke Brendan , Rohde Adele
Published: April 2021

GWMWater’s urban water customers can now better track their water usage on their phone, computer or any other electronic device following the completion of an Urban Remote Metering project in December 2020. Internet of Things (IoT) communication technology has been used to connect the 29,000 communications-enabled, all-in-one water meters and 3000 retrofitted meters to a secure network. Using this technology GWMWater is empowering rural and urban customers with pseudo real-time data in a Customer Portal so they can better understand their water use and adjust their habits where required.
Venatorbacter Cucullus Gen. Nov Sp. Nov a Novel Bacterial Predator
Authors: Saeedi Ahmed , Cummings Nicola , McLean Denise , Connerton Ian F. , Connerton Phillippa L.
Published: April 2021

A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5, was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently found to serve as prey. Weak axenic growth on Brain Heart Infusion agar was achieved upon subculture without host cells. The optimal growth conditions were 37°C, at pH 7. Transmission electron microscopy revealed some highly unusual morphological characteristics related to prey availability. Phylogenetic analyses using 16s rRNA gene sequences showed that the isolate was related to members of the Oceanospirillaceae family but could not be classified clearly as a member of any known genus. Whole genome sequencing of ASxL5 confirmed the relationship to members the Oceanospirillaceae. Database searches revealed that several ASxL5 share 16s rRNA gene sequences with several uncultured bacteria from marine, and terrestrial surface and subsurface water. We propose that strain ASxL5 represents a novel species in a new genus. We propose the name Venatorbacter cucullus gen. nov., sp. nov. with ASxL5 as the type strain.
Effect of The FOCUS Program on The Self-Efficacy of Caregivers for Patients With Advanced Lung Cancer
Authors: zhou qinxue , Huang Lihua , Yang Su , Han Hui , Shen Lijuan , Cai Jianli , Xing MeiYuan
Published: April 2021

Objective To investigate the effect of the FOCUS program on the self-efficacy of caregivers of patients with advanced lung cancer.Method In this experimental study, a total of 108 caregivers for patients admitted to a tertiary comprehensive hospital from April 2019 to December 2019 were recruited based on inclusion and exclusion criteria. Based on their admission dates, the caregivers were divided into a control group (54 cases), which only completed routine measures, and a test group (54 cases), which completed the FOCUS Program in addition to routine measures. At the 4th week of the intervention, general self-efficacy, caregiving burden, coping styles and mood states were compared between the control group and the test group.Results The general self-efficacy scale, caregiver burden scale, simple coping style scale and mood state scale were compared between the two groups at the 4th week of the intervention and at 8 weeks after the intervention.After the intervention, the self-efficacy scores of the test group (24.37±4.375) were higher than those of the control group (17.50±3.479), and the difference was statistically significant (t=-9.032, P<0.05); the caregiving burden scores of the test group (41.65± 2.735) were lower than those of the control group (47.33±7.066), and the difference was statistically significant (t=5.514, P<0.05); the coping style scores of the experimental group (33.20±5.533) were significantly higher than those of the control group (28.76±3.420) (t=-5.021, P<0.05); the mood state scale scores of the test group (106.17±12.400) were lower than those of the control group (127.65±11.754), and the difference was statistically significant (t=9.239, P<0.05).The results showed that the intergroup comparisons, group comparisons and interactions were statistically significant (P<0.05).Conclusion The FOCUS Program can effectively improve the self-efficacy of caregivers, reduce their caregiving burden improve their coping styles and enhance their mood states.
Efficacy of INtensive Treatment Versus Standard Treatment of COmpound DanshEn Dripping Pills in Refractory Angina Patients With Incomplete Revascularization (INCODER Study): Study Protocol for a Multicenter, Double-blind, Randomized Controlled, Superiority Trial
Authors: Wu Zexuan , Xu Danping , Wu Zhen , Chen Ailan , Xu Lin , Zhang Yuling , Ling Li , Zhou Yan , Liu Yin , Dong Yugang , Chen Yili
Published: April 2021

Introduction: Patients with incomplete revascularization (ICR) tend to develop refractory angina despite optimal medical therapy. Compound Danshen Dripping Pills (CDDP) is a widely used antianginal drug in China and is showed to significantly alleviate myocardial ischemia. Previous studies showed dose-efficacy tendency when increasing doses of CDDP. The study is aim to investigate the efficacy and safety of intensive doses of CDDP in patients with refractory angina with ICR.Methods and Analysis: The INCODER study is a multicenter, double-blind, randomized controlled, superiority trial. We plan to recruit 250 patients aged 18 to 85 years with a diagnosis of refractory angina with ICR. Patients will be randomized (1:1) to intensive treatment group (CDDP 20 pills three times per day) or control group (10 pills CDDP and 10 pills placebo three times per day). Patients will have a 6-week medication period and be followed up every two weeks. The primary end point is the change of total exercise time from baseline to week 6 as assessed by cardiopulmonary exercise testing (CPET). Secondary end points include changes in frequency of angina, Canadian Cardiovascular Society angina class, nitroglycerin use, Seattle Angina Questionnaire scores, O2 uptake kinetics and other parameters as measured by CPET, and levels of plasma C-reactive protein, homocysteine and N-terminal pro-B-type natriuretic peptide. Safety events related to CDDP use will be monitored.Ethics and dissemination: The research had been approved by the Clinical research and laboratory animal ethics committee of the First Affiliated Hospital, Sun Yat-sen University ([2019]65). The results will be reported through peer-reviewed journals, seminars and conference presentations.Trial registration number: www.chictr.org.cn (ChiCTR2000032384). Registered on 27 April 2020.
Hilbert' s First Problem and the New Progress of Infinity Theory
Authors: Wang Xijia
Published: April 2021

In the 19th century, Cantor created the infinite cardinal number theory based on the "1-1 correspondence" principle. The continuum hypothesis is proposed under this theoretical framework. In 1900, Hilbert made it the first problem in his famous speech on mathematical problems, which shows the importance of this question. We know that the infinitesimal problem triggered the second mathematical crisis in the 17-18th centuries. The Infinity problem is no less important than the infinitesimal problem. In the 21st century, Sergeyev introduced the Grossone method from the principle of "whole is greater than part", and created another ruler for measuring infinite sets. At the same time, the development of the infinity theory provides new ideas for solving Hilbert's first problem, and provides a new mathematical foundation for Cosmic Continuum Theory.
Fast Determination of Phosphorus Concentration in Phosphogypsum Waste Using Calibration-Free LIBS in Air and Helium
Authors: Elsayed Khaled , Tawfik Walid , Khater Ashraf E M , Kayed Tarek S , Fikry Mohamed
Published: April 2021

This work represents a novel method to determine phosphorus (P) concentration in phosphogypsum (PG) waste samples using calibration-free laser-induced breakdown spectroscopy (LIBS). A 50 mJ Q-switched Nd: YAG laser has generated the PG LIBS spectrum. Spectroscopic analysis of plasma evolution has been characterized by electron density Ne and electron temperature Te using the emission intensity and stark broadening for P I characteristic lines 213.61, 214.91, and 215.40 nm under non-purged (air) and purged (helium) conditions. It was found that both Te and Ne have significant changes linearly with P concentrations 4195, 5288, 6293, and 6905 ppm. The values of plasma Te and Ne increased from about 6900 to 10000 K and 1.1×1017 to 3.4×1017 cm− 3, respectively, for the non-purged PG. On the other hand, Te and Ne ranged from 8200 to 11000 K and 1.4×1017 to 3.5×1017 cm− 3, respectively, for the PG purged with helium. It is concluded that Te and Ne values represent a fingerprint plasma characterization for a given P concentration in PG samples, which can be used to identify P concentration without a PG's complete analysis. These results demonstrate a new achievement in the field of spectrochemical analysis of environmental applications.
First report of leaf spots caused by Pestalotiopsis trachicarpicola in Gentiana rhodantha
Authors: Zhang Xiaoyong , Yang Youlian , Li Shujiang , Yan Kai , Li Xinran
Published: April 2021

In August 2018, leaf spots were widely found with circular or irregular brown lesions on Gentiana rhodantha leaves in Liupanshui, Guizhou Province, China. Three strains of Pestalotiopsis spp were associated with leaf lesions by single-spore isolation. Based on asexual and sexual characteristics and multi-locus (ITS, TUB, tef-1 ) phylogenies data, strains were identified as Pestalotiopsis trachicarpicola . This is the first report of G. rhodantha leaf spot caused by P. trachicarpicola . The fungal culture tests showed that mycelia optimum growth temperature and pH were 20–25°C and pH 7–9, and for conidia germination were 25–30°C and pH 6–9, respectively.
Identification, Characterization, and Hydrolase Producing Performance of Thermophilic Bacteria: Geothermal Hot Springs in Eastern and Southeastern Anatolia Region of Turkey
Authors: Uluçay Orhan , Görmez Arzu , Öziç Cem
Published: April 2021

In the last twenty years, researchers have increasingly focused on the rich microorganism-based diversity of natural hot spring resources to explore the benefits of thermophiles in industrial and biotechnological fields. For this purpose, a total of 83 thermophilic bacilli were isolated from 7 different geothermal hot springs (at temperatures ranging between 40 and 85 ◦ C) located in the east and Southeastern of Turkey. These isolates were identified by different methods such as physiological, morphological, biochemical, molecular properties. According to 16S rRNA gene sequence analysis, 5 different isolates ( Bacillus coagulans , Bacillus licheniformis , Bacillus subtilis , Bacillus thuringiensis , and Geobacillus kaustophilus ) were identified. It was observed that B. licheniformis and B. subtilis were the most species obtained from the researched hot spring sources. Phylogenetic relationships of isolates were evaluated with the help of a phylogenetic tree. The conditions of bacterial isolates to synthesize various hydrolytic enzymes such as protease, cellulase, lipase, and amylase were investigated. When the potential of isolates to produce hydrolytic enzymes was examined, protease 73 (88%), cellulase 34 (41%), lipase 69 (83%), and amylase 68 (82%) were detected. All isolates have at least one or more extracellular protease, cellulase, amylase, or lipase activity. Besides, 32.8% (27) of bacterial isolates were able to synthesize all of the hydrolytic enzymes.
Dynamic Sensitivity Analysis of Deployable Flexible Space Structures With a Large Number of Design Parameters
Authors: Wang Shuai , Tian Qiang , Hu Haiyan , Shi Junwei , Zeng Lingbin
Published: April 2021

The reliability and dynamic performance of deployable flexible space structures significantly depend on their key design parameters. Sensitivity analysis of these design parameters in the frame of multibody dynamics can serve as a powerful tool to evaluate and improve the dynamic performances of deployable space structures. Nevertheless, previous studies on the dynamic sensitivity analysis are mainly confined to planar multibody systems with a few design parameters. In this study, an efficient computational methodology is proposed to perform the dynamic sensitivity analysis of the complex deployable space structures with a large number of design parameters. Firstly, the analytical sensitivity analysis formulations of objective functions with the parameter-dependent integration bounds are deduced via the direct differentiation method and adjoint variable method. A checkpointing scheme is further introduced to assist the backward integration of the high dimensional differential algebraic equations of the adjoint variables. The flexible beams in the deployable flexible space structure are described by the locking-free three-node spatial beam elements of absolute nodal coordinate formulation. Furthermore, a parallelized automatic differentiation algorithm is proposed to efficiently evaluate the complex partial derivatives in the sensitivity analysis formulations. Finally, four numerical examples are provided to validate the accuracy and efficiency of the proposed computational methodology.
Effect of Gate Metal Work-function on Junctionless (JL) Nanowire GAA MOSFET Performance
Authors: Angara Manoj , Jena Biswajit , Rooban S.
Published: April 2021

Metal gate technology is one of the promising methods used to increase the drain current by increasing the electrostatic controllability. Different metals have different work-function that controls the device performance very closely as gate to source voltage is the basic inputs for these. In this paper the dependency of gate metal work-function on device performance (both for nMOS and pMOS) is extensively investigated. The gate metal work-function value is taken as 4.2eV to 5.1eV with one increment to see the change in potential profile. With this condition, the IOn current, IOff current, threshold voltage, transconductance also calculated for these structures. A decrease value in drain current (1e-6 to 1e-7 A) is observed for both the cases with increase in work-function of gate metal. However, the Off current is getting better (1e-7 to 1e-18 A) while moving towards higher metal work-function values. As a result of which the IOn/IOff ratio increases which leads to higher device performances.