bluuebunny/crossref_metadata_2025 huggingface dataset:
Positive and Negative Work Practices of Forest Machine Operators: Interviews and Literature Analysis
Authors: Hartsch Florian , Dreger Felix A. , Englund Martin , Hoffart Even , Rinkenauer Gerhard , Wagner Thilo , Jaeger Dirk
Published: December 2022

Variance in productivity of fully mechanized timber harvesting under comparable stand and terrain conditions requires the investigation of the influence of work practices of machine operators. Work practices can vary among operators and may result in a wide range of productivity. Therefore, it is of great interest to identify positive and negative work practices of forest machine operators to improve forest work. For the qualitative analysis of work practices, 15 forest machine operator instructors were interviewed in Norway, Sweden, and Germany in semi-structured interviews. Additionally, a literature review following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was performed. The interviews brought up detailed positive work practices and showed negative examples of machine handling, specifically related to boom operation. The literature review retrieved 2482 articles of which 16 were examined in more detail. The review showed that work practice characteristics were only sparsely covered, however, still overlapped with the work practice recommendations from the operator instructor interviews. Further, the literature search unveiled a scientific knowledge gap related to the quantification of applied work practices. Generally, positive work practices can include using optimal working ranges from 4–6 m, frequent machine repositioning, a matched fit of operator skill and crane speed, and an assortment pile size that matches the maximum grapple loads. Training is recommended to focus on crane control in terms of movement precision and work range adherence whereby the speed-accuracy trade-off should be improved to meet productivity requirements and increase efficiency in forest machine operator work.
Comparative Analysis of Skeleton-Based Human Pose Estimation
Authors: Chung Jen-Li , Ong Lee-Yeng , Leow Meng-Chew
Published: December 2022

Human pose estimation (HPE) has become a prevalent research topic in computer vision. The technology can be applied in many areas, such as video surveillance, medical assistance, and sport motion analysis. Due to higher demand for HPE, many HPE libraries have been developed in the last 20 years. In the last 5 years, more and more skeleton-based HPE algorithms have been developed and packaged into libraries to provide ease of use for researchers. Hence, the performance of these libraries is important when researchers intend to integrate them into real-world applications for video surveillance, medical assistance, and sport motion analysis. However, a comprehensive performance comparison of these libraries has yet to be conducted. Therefore, this paper aims to investigate the strengths and weaknesses of four popular state-of-the-art skeleton-based HPE libraries for human pose detection, including OpenPose, PoseNet, MoveNet, and MediaPipe Pose. A comparative analysis of these libraries based on images and videos is presented in this paper. The percentage of detected joints (PDJ) was used as the evaluation metric in all comparative experiments to reveal the performance of the HPE libraries. MoveNet showed the best performance for detecting different human poses in static images and videos.
Coordinated Development of the Marine Environment and the Marine Fishery Economy in China, 2011–2020
Authors: Liu Yang , Jiang Yiying , Pei Zhaobin , Han Limin , Shao Hongrun , Jiang Yang , Jin Xiaomeng , Tan Saihong
Published: December 2022

The marine environment is the material basis for the survival and development of fishery resources, and changes in the marine environment affect the fishery economy. Therefore, against the background of sustainability and environmental uncertainty, it is important to investigate the development of the marine environment and the marine fishery economy to improve the quality of both. Taking the panel data for 11 coastal cities in China from 2011 to 2020, we use several methods, including the entropy method, a coupling harmonious degree model, and a Tobit model, to measure the marine-environment quality and marine-fishery-economy quality, their coordination, and the factors affecting that coordination. We find that (1) the marine-environment quality and marine-fishery economy quality show a significant upward trend over time, but they are spatially unbalanced, with obvious interprovincial differences. (2) Coordination between the marine-environment quality and marine-fishery-economy quality has risen steadily, but the level of coordination is still low, remaining at the primary level in most areas. (3) The important factors affecting coordination between the marine-environment quality and marine-fishery-economy quality include the strength of the marine fishery industry, scale of the marine fishery economy, production capacity of marine fisheries, marine-environment quality, and quality of the marine environment and its resources. In light of these findings, we should increase the coordination between the marine-environment quality and marine-fishery-economy quality by upgrading the marine fishery industry, modernizing marine fisheries, linking the environmental governance of marine and land areas, and strengthening the ecological construction of the marine environment.
Current Advances in 3D Dynamic Cell Culture Systems
Authors: Huang Xin , Huang Zhengxiang , Gao Weidong , Gao Wendong , He Ruiying , Li Yulin , Crawford Ross , Zhou Yinghong , Xiao Lan , Xiao Yin
Published: December 2022

The traditional two-dimensional (2D) cell culture methods have a long history of mimicking in vivo cell growth. However, these methods cannot fully represent physiological conditions, which lack two major indexes of the in vivo environment; one is a three-dimensional 3D cell environment, and the other is mechanical stimulation; therefore, they are incapable of replicating the essential cellular communications between cell to cell, cell to the extracellular matrix, and cellular responses to dynamic mechanical stimulation in a physiological condition of body movement and blood flow. To solve these problems and challenges, 3D cell carriers have been gradually developed to provide a 3D matrix-like structure for cell attachment, proliferation, differentiation, and communication in static and dynamic culture conditions. 3D cell carriers in dynamic culture systems could primarily provide different mechanical stimulations which further mimic the real in vivo microenvironment. In this review, the current advances in 3D dynamic cell culture approaches have been introduced, with their advantages and disadvantages being discussed in comparison to traditional 2D cell culture in static conditions.
A Comparative Transcriptomic with UPLC-Q-Exactive MS Reveals Differences in Gene Expression and Components of Iridoid Biosynthesis in Various Parts of Gentiana macrophylla
Authors: Kou Yuhang , Yi Xiaoying , Li Zhuo , Ai Yun , Ma Siting , Chen Qianliang
Published: December 2022

Gentiana macrophylla Pall. (G. macrophylla)—a member of the family Gentianaceae—is a well-known traditional Chinese medical herb. Iridoids are the main active components of G. macrophylla, which has a wide range of pharmacological activities such as dispelling wind, eliminating dampness, clearing heat and asthenic fever, hepatoprotective and choleretic actions, and other medicinal effects. In this study, a total of 67,048 unigenes were obtained by transcriptomic sequencing analysis of G. macrophylla. A BLAST analysis showed that 48.21%, 33.66%, 46.32%, and 32.62% of unigenes were identified in the NR, Swiss-Prot, eggNOG, and KEGG databases, respectively. Twenty-five key enzymes were identified in the iridoid biosynthesis pathway. Most of the upregulated unigenes were enriched in flowers and leaves. The trustworthiness of the transcriptomic data was validated by real-time quantitative PCR (qRT-PCR). A total of 22 chemical constituents were identified by ultra-high performance liquid chromatography-quadrupole-electrostatic field Orbitrap mass spectrometry (UPLC-Q-Exactive MS), including 10 iridoids. A correlation analysis showed that the expression of 7-DLH and SLS was closely related to iridoids. The expression of 7-DLH and SLS was higher in flowers, indicating that flowers are important for iridoid biosynthesis in G. macrophylla.
How Great Was the “Great Divide of Nature and Culture” in Europe? Philippe Descola’s Argument under Scrutinity
Authors: Mathieu Jon
Published: December 2022

In his much-discussed work Beyond Nature and Culture, anthropologist Philippe Descola gives central importance to the “great divide” between nature and culture in European history. According to him, the “naturalism” created by this gap is at the heart of Western modernity and distinguishes it from the “others” on the planet. One can certainly agree with Descola that the nature-culture dualism cannot claim universal validity. However, the extent of the “great divide” created in Europe by early modern “rationalist” scholarship remains unclear. Methodologically, one should not limit oneself to the narrow history of science and philosophy, but also examine the linguistic, religious, and social history.
Comparison of Different Physical Methods and Preservatives for Control of Fusarium proliferatum Rot in Garlic
Authors: La Placa Laura , Giorni Paola , Mondani Letizia , Magan Naresh , Battilani Paola
Published: December 2022

Dry rot is an emerging issue for garlic production worldwide and Fusarium proliferatum is its major causal agent. Since the disease is seed-transmitted, sowing healthy cloves is crucial. In this study, some disinfection strategies were tested on garlic seeds, including steam, dry heat, chemical disinfectants and gaseous ozone (O3). Steam reduced the Colony Forming Units·g−1 (CFUs·g−1) by up to 92% in garlic seeds, but, at the same time, it affected their germination (−36%). Similarly, hydrogen peroxide (H2O2) and peracetic acid (C2H4O3) reduced the CFUs·g−1 by up to 83%; however, these methods also severely impaired germination (−40%). Dry heat did not negatively impact germination, but fungal contamination was not significantly reduced. The most promising strategy was gaseous O3 treatment; it decreased CFUs·g−1 by up to 96%, without causing any reduction of germination. The treatments applied were partially effective because the fungus is predominantly located in the outer layer of the seed, although it is also found in the inner portions. Some of these treatments can contribute to garlic protection from seed-borne pathogens and possibly reduce the occurrence of garlic dry rot.
Multitiered Fresh Produce Supply Chain: The Case of Tomatoes
Authors: Cui Xiurui , Guan Zhengfei , Morgan Kimberly , Huang Kuan-Ming , Hammami A.
Published: December 2022

The United States is one of the world’s top producers of fresh tomatoes. However, the industry supply chain is not well understood with little attention in the literature. To attend to this literature gap and to better inform business decisions and policy making, it is crucial to understand the industry structure on a deeper level. Applying a supply chain mapping approach, this study presents a complete picture of the U.S. fresh tomato supply chain with valuable insights into the fresh produce industry and lays the groundwork for future research on solving supply chain issues.
Exploring the Digital Divide among the Bhutanese Refugee Community during COVID-19: Engaged Research in Action
Authors: Cohen Jeffrey H. , Maleku Arati , Pyakurel Sudarshan , Suzuki Taku , Raut Shambika , Montiel Ishino Francisco Alejandro
Published: December 2022

The digital divide proved a critical barrier to accessing information and healthcare during the COVID-19 pandemic and negatively impacted the Bhutanese refugee community. Moving beyond a technological model of the digital divide that highlights a lack of access to computers and the internet, we engaged the community to co-produce a dynamic approach that identifies the impact of socio-cultural and socio-environmental factors as well. Our paper reports on our community-academic research partnership and explores how the digital divide exacerbates health disparities in a midwestern Bhutanese refugee community. Combining the efforts of the community, anthropologists and social workers, this paper reports on the health disparities that confront the community as well as interventions designed to mitigate social inequities.
Co-Design of an Educational Resource with Female Partners of Male Stroke Survivors to Support Physical Activity Participation
Authors: Calder Allyson , Sole Gisela , Mulligan Hilda
Published: December 2022

Many male stroke survivors find it challenging to meet the recommended physical activity (PA) guidelines for health benefits. The spouse/partner is an important source of self-management for stroke survivor PA participation; however, they feel unsupported by health professionals. This study aimed to co-design an educational resource prototype to guide and empower female partners in supporting male stroke survivors’ participation in PA. We used a participatory action research (PAR) methodology. Thirteen support persons of male stroke survivors from Canterbury, New Zealand participated in four PAR cycles. The data were collected using individual interviews and focus groups and analyzed inductively using the general inductive approach. Three themes were reflected in the data and informed the prototype content: (1) managing an unwanted and challenging new life, (2) inconsistent access to meaningful information, and (3) considerations for successful stroke survivor PA participation. If partners are to be an essential source in supporting stroke survivors’ self-management of PA, they require resources that are meaningful and credible to enhance their confidence and self-efficacy. Further research is needed to explore the acceptability and usability of the educational resource with a wider audience and evaluate the co-design process. An inclusive and collaborative approach where support persons were valued for their expertise was essential in co-designing a meaningful resource intended to support stroke survivors and support persons’ self-management of their PA.
Parental Influence on Child and Adolescent Physical Activity Level: A Meta-Analysis
Authors: Su Diana L. Y. , Tang Tracy C. W. , Chung Joan S. K. , Lee Alfred S. Y. , Capio Catherine M. , Chan Derwin K. C.
Published: December 2022

Parents are often regarded as one of the significant social agents who are important to the participation of physical activity (PA) among children and adolescents. However, within the literature, the relationships between parental influences and child and adolescent PA have been inconclusive and discordant. The purpose of this meta-analysis was to quantify and synthesize the associations between parental social influences (positive parental influence, punishment, and discouragement) and the PA level of children and adolescents. Through a systematic literature search using PsycINFO, Web of Science, PubMed, ProQuest, and SPORTDiscus databases, we identified 112 eligible studies and subsequently extracted 741 effect sizes for our analysis. Multilevel meta-analysis showed that the corrected zero-order correlation of positive parental influence was positive and statistically significant, r = 0.202, SE = 0.014, t = 14.975, p < 0.001, 95% confidence interval (CI) = [0.176, 0.228]. Further moderation analysis also found that this was significantly moderated by parental gender (maternal vs. paternal), respondent of influence measure (parent-reported vs. child-reported), and type of PA measure (subjective vs. objective). The corrected zero-order correlations of negative parental influences (i.e., punishment and discouragement) were not statistically significant, and no significant moderation effects were observed. The findings of our meta-analysis showed that children and adolescents had higher PA levels when their parents supported PA participation by exerting positive social influence. Punishment and discouragement against PA by parents did not appear to be significantly associated with the PA level of children and adolescents. The findings of negative parental social influence were mixed and required further investigations.
Assessment of Both Personal and Professional Aspects to Measure Job Satisfaction Levels among Garment Workers: Empirical Evidence from a Developing Country
Authors: Yuan Deli , Gazi Md. Abu Issa , Rahman Md. Alinoor
Published: December 2022

The main objective of the present study is to assess the role of professional and personal aspects in determining job satisfaction of garment industry workers in Bangladesh as a developing country. The present study is conducted on 350 workers from 25 garment factories in Dhaka, Narayanganj, and Gazipur, three districts of Bangladesh where the garment industry dominates. The study location and sample size were determined based on the random sampling method. All the participating workers were interviewed in the light of a predetermined questionnaire. Statistical Package for Social Science-SPSS software, version 24.0 was used for data analysis. Brayfield–Rothe Job Satisfaction Measurement Scale (JSMS) and Questionnaire for Measuring Satisfaction with professional aspects (QMSPA) has been used in this study. The results of the current study showed that, among personal aspects, only age significantly impacted the respondents’ overall job satisfaction. The respondents’ overall job satisfaction was also significantly influenced by all professional factors. Between the personal and professional facets, the importance of professional factors as determinants of job satisfaction is greater than that of personal factors. The results of the present study suggest that since professional aspects are the main components of job satisfaction, it is necessary to ensure the proper presence of these components. Factors such as pay, job security, and adequate and appropriate amount are indicators of job satisfaction. All parties involved in the garments industry such as employer–worker, regulatory body BGMEA, BKMEA, policy-making body, government, and factory authorities can take into account the results of this study and determine future course of action to increase workers’ job satisfaction.
The Effects of the COVID-19 Pandemic on the Parenting of Infants: A Couples Study
Authors: Anikiej-Wiczenbach Paulina , Kaźmierczak Maria
Published: December 2022

(1) Background: The COVID-19 pandemic has led to many negative changes in everyday functioning. This study aimed to establish how it impacts parental responsiveness towards their children; (2) Methods: 132 couples (N = 264) who were parents of young children (from 3 to 24 months; M = 12.61; SD = 6.71) participated in this study. The Parental Responsiveness Scale was used to measure parental responsiveness toward their own child and the Polish adaptation of the My Emotions Scale was used to measure emotional reactions to the child’s cry. We collected data about perceived stress, fear of being affected by COVID-19, and emotional overload caused by the pandemic. An analysis using actor–partner interdependence models was carried out; (3) Results: there were actor effects for both parental responsiveness and reactions to the child’s cry (for all measured aspects (frustration, amusement, anxiety, empathy, sympathy)). For women, parental responsiveness was a negative partner effect of stress, and for men, there was a positive effect of fear of being infected, emotional overload, and stress; (4) Conclusions: these results show how important it is to take care of families and investigate the effects of the pandemic on their functioning.
Effects of Exercise Training on Neurotrophic Factors and Blood–Brain Barrier Permeability in Young-Old and Old-Old Women
Authors: Cho Su-Youn , Roh Hee-Tae
Published: December 2022

Aging and regular exercise may have opposite effects on brain health, and although oxidative stress and sirtuins may be involved in these effects, studies on this topic are limited. Accordingly, the present study aimed to verify the effect of exercise training on oxidant–antioxidant balance, neurotrophic factors, blood–brain barrier permeability, and sirtuins in young-old and old-old women. The study participants were 12 women aged 65–74 years (Young-Old group) and 12 women aged 75–84 years (Old-Old group). All of the selected participants performed exercise training consisting of treadmill walking and resistance band exercise three times a week for 12 weeks. Blood samples were collected before and after exercise training to analyze serum oxidant–antioxidant markers (reactive oxygen species [ROS], superoxide dismutase [SOD]), neurotrophic factor (brain-derived neurotrophic factor [BDNF], vascular endothelial growth factor [VEGF]) levels, and blood–brain barrier permeability marker (S100 calcium-binding protein β [S100β], matrix metalloproteinase-9 [MMP-9]) levels, and sirtuin (SIRT-1, SIRT-2, SIRT-3) levels. The Young-Old group showed significantly increased SOD, BDNF, VEGF, SIRT-1, and SIRT-3 levels after training in comparison with the levels before training (p < 0.05), and a significantly higher BDNF level than the Old-Old group after training (p < 0.05). On the other hand, the Old-Old group showed significantly higher SIRT-1 levels after training in comparison with the levels before training (p < 0.05). Thus, exercise training may be effective in increasing the levels of neurotropic factors and reducing blood–brain barrier permeability in the elderly women, and increased antioxidant capacity and elevated levels of sirtuins are believed to play a major role in these effects. The positive effect of exercise may be greater in participants of relatively young age.
Ecological Quality Evolution and Its Driving Factors in Yunnan Karst Rocky Desertification Areas
Authors: Zhang Shiwen , Wang Yan , Wang Xuehua , Wu Yang , Li Chengrong , Zhang Chao , Yin Yuhang
Published: December 2022

Rocky desertification is a key element affecting regional ecological quality. Rocky desertification in Southwest China directly affects the ecological security of the Yangtze River and Pearl River basins and also restricts regional economic and social development. In order to clarify the evolution laws and key influencing factors of ecological quality in Yunnan karst rocky desertification areas, a quantitative analysis based on the remote sensing-based ecological index (RSEI) model was conducted to explore the overall evolution characteristics and change laws of ecological quality in Yunnan karst rocky desertification areas in the past 30 years. The correlation between RSEI, rock outcrop rate (Fr), and driving factors was determined by redundancy analysis. The results showed the following: (1) RSEI in Yunnan karst rocky desertification areas generally showed a decreasing trend, with a fluctuation in the mid-term, followed by a tendency to recover. It fell into three stages: decline, trough, and recovery, with fitting coefficients of −0.121, −0.057, and 0.157, respectively. In contrast, Fr showed an opposite tendency, illustrating the inverse relationship between RSEI and Fr, and the rate of sequential succession was much faster than that of the reverse succession under human measures of intervention. (2) The mean value of RSEI of Yunnan karst rocky desertification areas was generally lower than that of the total Yunnan Province land areas and Yunnan non-karst rocky desertification areas, but the mean value of Fr was generally more than that of both the above-mentioned areas. In addition, the RSEI and Fr of Yunnan karst rocky desertification areas both showed lower stability values than those of both the above-mentioned areas. This generally suggested a low ecological quality and a high degree of desertification under a low stability in Yunnan karst rocky desertification areas. (3) The correlation of RSEI and Fr with driving factors followed the order of topographic factors, soil factors > water factors > anthropogenic factors. Anthropogenic factors were the driving force changing the state of rocky desertification, geological factors such as topography and soil to a larger extent determined the original macroscopic ecological relationship of rocky desertification, and water factors lay between the above two. The findings of this research will provide theoretical support and a basis for the improvement of ecological quality and comprehensive control of karst rocky desertification in Yunnan Province.
Relational Victimization and Video Game Addiction among Female College Students during COVID-19 Pandemic: The Roles of Social Anxiety and Parasocial Relationship
Authors: Niu Gengfeng , Jin Siyu , Xu Fang , Lin Shanyan , Zhou Zongkui , Longobardi Claudio
Published: December 2022

Video game addiction, a common behavioral problem among college students, has been more prominent during the COVID-19 pandemic; at the same time, females’ video game usage has also attracted considerable research attention. Against this background and under the perspective of social interaction, this study aimed to examine the relationship between relational victimization and video game addiction among female college students, as well as its underlying mechanism—the mediating roles of social anxiety and parasocial relationships with virtual characters. Female college students (N = 437) were recruited to complete a set of questionnaires voluntarily in June 2022. Through the mediating effect analysis, the results found that (1) relational victimization was positively associated with female college students’ video game addiction; (2) social anxiety and parasocial relationships with virtual characters could independently mediate this relation; (3) social anxiety and parasocial relationships with virtual characters were also the serial mediators in this association. These findings not only expand previous studies by revealing the social motivation of video game usage and the underlying mechanism accounting for video game addiction, but also provide basis and guidance for the prevention and intervention of video game addiction in the current context of the COVID-19 pandemic.
Morphometric Analysis of the Midline Mandibular Lingual Canal and Mandibular Lingual Foramina: A Cone Beam Computed Tomography (CBCT) Evaluation
Authors: Alqutaibi Ahmed Yaseen , Alassaf Muath Saad , Elsayed Shadia A. , Alharbi Abdulmajeed Saud , Habeeb Abdulsamad Talaat , Alqurashi Marwan Ahmad , Albulushi Khalid Ahmed , Elboraey Mohamed Omar , Alsultan Kamal , Mahmoud Ihab Ismail
Published: December 2022

Background: This study aimed to evaluate the midline mandibular lingual canals and foramina and their anatomic variations using CBCT scans. Methods: This study used retrospective analysis. A total of 320 CBCT scans were used to evaluate the study parameters, which comprised the presence or absence of the mandibular lingual foramen (MLF)/mandibular lingual canal (MLC) and its category, the distance between the buccal cortex and the start of the MLC, the distance between the inferior border of the mandible and the superior border of the foramen at its lingual and buccal terminals. The length and diameter of each canal at its lingual and buccal terminals. Results: MLC was found in all included CBCT scans. Out of 320 included CBCT scans, a single canal was represented by 30.9%, double canals (Supra with Infra -spinosum) configuration appeared in 54.7%, and triple canals (Supra-Inter-Infra) represented 14.7%. The supraspinosum canals averaged 5.81 ± 2.08 mm in length and 0.87 ± 0.30 mm in diameter at the lingual terminal. In terms of the number of canals, there was a significant difference between men and women (p ≤ 0.001), with 60% of the men in the sample having double canals and 43.1% of the women having single canals. Moreover, the male gender had a higher prevalence of triple canals (21.3% vs. 8.1%) than females. Males and females were distributed equally among the supraspinosum canals, with no statistically significant difference (p ≤ 0.7). A considerable increase in the finding of interspinosum and infraspinosum canals was seen in the male sample (p ≤ 0.001). Conclusions: midline mandibular canals were found in all investigated CBCTs of the sample of both sexes; however, the anatomy and location of the MLF and canals varied significantly among the Saudi population.
Do Greener Urban Streets Provide Better Emotional Experiences? An Experimental Study on Chinese Tourists
Authors: Zhang Yanyan , Wang Meng , Li Junyi , Chang Jianxia , Lu Huan
Published: December 2022

Compared to the usual environment, the potential momentary emotional benefits of exposure to street-level urban green spaces (UGS) in the unusual environment have not received much academic attention. This study applies an online randomized control trial (RCT) with 299 potential tourists who have never visited Xi’an and proposes a regression model with mixed effects to scrutinize the momentary emotional effects of three scales (i.e., small, medium and large) and street types (i.e., traffic lanes, commercial pedestrian streets and culture and leisure walking streets). The results identify the possibility of causality between street-level UGS and tourists’ momentary emotional experiences and indicate that tourists have better momentary emotional experiences when urban streets are intervened with large-scale green vegetation. The positive magnitude of the effect varies in all three types of streets and scales of intervention, while the walking streets with typical cultural attractions, have a larger impact relative to those with daily commute elements. These research results can provide guidance for UGS planning and the green design of walking streets in tourism.
Biodegradable Polyesters and Low Molecular Weight Polyethylene in Soil: Interrelations of Material Properties, Soil Organic Matter Substances, and Microbial Community
Authors: Šerá Jana , Huynh Florence , Ly Faith , Vinter Štěpán , Kadlečková Markéta , Krátká Vendula , Máčalová Daniela , Koutný Marek , Wallis Christopher
Published: December 2022

Conventional and also biodegradable polymer microplastics have started to be broadly present in the environment, if they end up in soil, they may influence both abiotic and biotic soil properties. In this study, the interactions of polyethylene wax together with three biodegradable polyesters PLA, PHB and PBAT with a soil matrix were investigated over a 1-year incubation period. Soil organic matter content was measured using UV–VIS, the microbial biomass amount was measured using qPCR, the mineralisation of polymers was measured using UGA 3000, the surface of polymers was observed with SEM, live/dead microorganisms were determined by fluorescent microscopy and microbial consortia diversity was analyzed using NGS. The amount of humic substances was generally higher in incubations with slowly degrading polyesters, but the effect was temporary. The microbial biomass grew during the incubations; the addition of PHB enhanced fungal biomass whereas PE wax enhanced bacterial biomass. Fungal microbial consortia diversity was altered in incubations with PHB and PBAT. Interestingly, these two polyesters were also covered in biofilm, probably fungal. No such trend was observed in a metagenomic analysis of bacteria, although, bacterial biofilm was probably formed on the PE520 surface. Different methods confirmed the effect of certain polymers on the soil environment.
Decreased Innate Migration of Pro-Inflammatory M1 Macrophages through the Mesothelial Membrane Is Affected by Ceramide Kinase and Ceramide 1-P
Authors: Ku Chee Wai , Yang Joan , Tan Hong Ying , Chan Jerry Kok Yen , Lee Yie Hou
Published: December 2022

The retrograde flow of endometrial tissues deposited into the peritoneal cavity occurs in women during menstruation. Classically (M1) or alternatively (M2) activated macrophages partake in the removal of regurgitated menstrual tissue. The failure of macrophage egress from the peritoneal cavity through the mesothelium leads to chronic inflammation in endometriosis. To study the migration differences of macrophage phenotypes across mesothelial cells, an in vitro model of macrophage egress across a peritoneal mesothelial cell monolayer membrane was developed. M1 macrophages were more sessile, emigrating 2.9-fold less than M2 macrophages. The M1 macrophages displayed a pro-inflammatory cytokine signature, including IL-1α, IL-1β, TNF-α, TNF-β, and IL-12p70. Mass spectrometry sphingolipidomics revealed decreased levels of ceramide-1-phosphate (C1P), an inducer of migration in M1 macrophages, which correlated with its poor migration behavior. C1P is generated by ceramide kinase (CERK) from ceramide, and blocking C1P synthesis via the action of NVP231, a specific CERK chemical inhibitor, prohibited the emigration of M1 and M2 macrophages up to 6.7-fold. Incubation with exogenously added C1P rescued this effect. These results suggest that M1 macrophages are less mobile and have higher retention in the peritoneum due to lower C1P levels, which contributes to an altered peritoneal environment in endometriosis by generating a predominant pro-inflammatory cytokine environment.
MicroRNAs: A Link between Mammary Gland Development and Breast Cancer
Authors: Wu Diana , Thompson Lilian U. , Comelli Elena M.
Published: December 2022

Breast cancer is among the most common cancers in women, second to skin cancer. Mammary gland development can influence breast cancer development in later life. Processes such as proliferation, invasion, and migration during mammary gland development can often mirror processes found in breast cancer. MicroRNAs (miRNAs), small, non-coding RNAs, can repress post-transcriptional RNA expression and can regulate up to 80% of all genes. Expression of miRNAs play a key role in mammary gland development, and aberrant expression can initiate or promote breast cancer. Here, we review the role of miRNAs in mammary development and breast cancer, and potential parallel roles. A total of 32 miRNAs were found to be expressed in both mammary gland development and breast cancer. These miRNAs are involved in proliferation, metastasis, invasion, and apoptosis in both processes. Some miRNAs were found to have contradictory roles, possibly due to their ability to target many genes at once. Investigation of miRNAs and their role in mammary gland development may inform about their role in breast cancer. In particular, by studying miRNA in development, mechanisms and potential targets for breast cancer treatment may be elucidated.
Fluoridated Apatite Coating on Human Dentin via Laser-Assisted Pseudo-Biomineralization with the Aid of a Light-Absorbing Molecule
Authors: Oyane Ayako , Sakamaki Ikuko , Nakamura Maki , Koga Kenji , Shitomi Kanako , Tanaka Saori , Miyaji Hirofumi
Published: December 2022

A simple, area-specific coating technique for fluoridated apatite (FAp) on teeth would be useful in dental applications. Recently, we achieved area-specific FAp coating on a human dentin substrate within 30 min by a laser-assisted biomimetic (LAB) process; pulsed Nd:YAG laser irradiation in a fluoride-containing supersaturated calcium phosphate solution (FCP solution). The LAB-processed, FAp-coated dentin substrate exhibited antibacterial activity against a major oral bacterium, Streptococcus mutans. In the present study, we refined the LAB process with a combination of a dental diode laser and a clinically approved light-absorbing molecule, indocyanine green (ICG). A micron-thick FAp layer was successfully formed on the dentin surface within only 3 min by the refined LAB process, i.e., dental diode laser irradiation in the FCP solution following ICG treatment. The ICG layer precoated on the dentin substrate played a crucial role in inducing rapid pseudo-biomineralization (FAp layer formation) on the dentin surface by absorbing laser light at the solid-liquid interface. In the refined LAB process, the precoated ICG layer was eliminated and replaced with the newly formed FAp layer composed of vertically oriented pillar-like nanocrystals. Cross-sectional ultrastructural analysis revealed a smooth interface between the FAp layer and the dentin substrate. The refined LAB process has potential as a tool for the tooth surface functionalization and hence, is worth further process refinement and in vitro and in vivo studies.
Functional and Taxonomic Traits of the Gut Microbiota in Type 1 Diabetes Children at the Onset: A Metaproteomic Study
Authors: Levi Mortera Stefano , Marzano Valeria , Vernocchi Pamela , Matteoli Maria Cristina , Guarrasi Valerio , Gardini Simone , Del Chierico Federica , Rapini Novella , Deodati Annalisa , Fierabracci Alessandra , Cianfarani Stefano , Putignani Lorenza
Published: December 2022

Type 1 diabetes (T1D) is a chronic autoimmune metabolic disorder with onset in pediatric/adolescent age, characterized by insufficient insulin production, due to a progressive destruction of pancreatic β-cells. Evidence on the correlation between the human gut microbiota (GM) composition and T1D insurgence has been recently reported. In particular, 16S rRNA-based metagenomics has been intensively employed in the last decade in a number of investigations focused on GM representation in relation to a pre-disease state or to a response to clinical treatments. On the other hand, few works have been published using alternative functional omics, which is more suitable to provide a different interpretation of such a relationship. In this work, we pursued a comprehensive metaproteomic investigation on T1D children compared with a group of siblings (SIBL) and a reference control group (CTRL) composed of aged matched healthy subjects, with the aim of finding features in the T1D patients’ GM to be related with the onset of the disease. Modulated metaproteins were found either by comparing T1D with CTRL and SIBL or by stratifying T1D by insulin need (IN), as a proxy of β-cells damage, showing some functional and taxonomic traits of the GM, possibly related to the disease onset at different stages of severity.
Nucleolar Stress Response via Ribosomal Protein L11 Regulates Topoisomerase Inhibitor Sensitivity of P53-Intact Cancers
Authors: Ishihara Yuka , Nakamura Kiyoshiro , Nakagawa Shunsuke , Okamoto Yasuhiro , Yamamoto Masatatsu , Furukawa Tatsuhiko , Kawahara Kohichi
Published: December 2022

Nucleolar stress response is caused by perturbations in ribosome biogenesis, induced by the inhibition of ribosomal RNA processing and synthesis, as well as ribosome assembly. This response induces p53 stabilization and activation via ribosomal protein L11 (RPL11), suppressing tumor progression. However, anticancer agents that kill cells via this mechanism, and their relationship with the therapeutic efficiency of these agents, remain largely unknown. Here, we sought to investigate whether topoisomerase inhibitors can induce nucleolar stress response as they reportedly block ribosomal RNA transcription. Using rhabdomyosarcoma and rhabdoid tumor cell lines that are sensitive to the nucleolar stress response, we evaluated whether nucleolar stress response is associated with sensitivity to topoisomerase inhibitors ellipticine, doxorubicin, etoposide, topotecan, and anthracyclines. Cell proliferation assay indicated that small interfering RNA-mediated RPL11 depletion resulted in decreased sensitivity to topoisomerase inhibitors. Furthermore, the expression of p53 and its downstream target proteins via western blotting showed the suppression of p53 pathway activation upon RPL11 knockdown. These results suggest that the sensitivity of cancer cells to topoisomerase inhibitors is regulated by RPL11-mediated nucleolar stress responses. Thus, RPL11 expression may contribute to the prediction of the therapeutic efficacy of topoisomerase inhibitors and increase their therapeutic effect of topoisomerase inhibitors.
Induction of Apoptosis by Matrine Derivative ZS17 in Human Hepatocellular Carcinoma BEL-7402 and HepG2 Cells through ROS-JNK-P53 Signalling Pathway Activation
Authors: Wang Xiang , Zhang Sen , Han Keyan , Wang Lisheng , Liu Xu
Published: December 2022

Hepatocellular carcinoma (HCC) is one of the most common malignancies and ranks third among cancer-related deaths worldwide. Using matrine as a lead compound, 12 matrine derivatives were designed and synthesised, and their antiproliferative activities were evaluated in four cancer cell lines. Eight of the twelve compounds showed strong antiproliferative activity, with an IC50 of <10 μM. The compound ZS17 exhibited strong antiproliferative activity in hepatocellular carcinoma cell lines with IC50 values in the range of 3.014–3.388 μM, which was much lower than that of matrine. Furthermore, we explored the role of ZS17 in inducing apoptosis in HCC cells in vitro and in vivo, as well as possible mechanisms involved. ZS17 inhibited the proliferation of BEL-7402 and HepG2 cells in time- and dose-dependent manners. In addition, we found that ZS17 significantly induced apoptosis and ROS (reactive oxygen species) production, promoted JNK phosphorylation, activated p53, and activated the caspase signalling pathway. Furthermore, the antioxidant NAC, JNK inhibitor SP600125, and Si-JNK increased cell viability, re-established cell metastasis, and inhibited ZS17-induced apoptosis. An in vivo antitumour assay demonstrated that ZS17 significantly reduced the number of migrating HepG2 cells in zebrafish embryos and suppressed the growth of HepG2 xenografts in nude mice without any obvious side effects. Our study demonstrated that the ROS-JNK-P53 pathway plays an important role in the destruction of liver tumour cells by ZS17. Thus, ZS17 may represent a promising chemotherapeutic agent for the treatment of HCC patients.
The Reactions of N,N′-Diphenyldithiomalondiamide with Arylmethylidene Meldrum’s Acids
Authors: Dotsenko Victor V. , Aksenov Alexander V. , Sinotsko Anna E. , Varzieva Ekaterina A. , Russkikh Alena A. , Levchenko Arina G. , Aksenov Nicolai A. , Aksenova Inna V.
Published: December 2022

The Michael addition reaction between dithiomalondianilide (N,N′-diphenyldithiomalondiamide) and arylmethylidene Meldrum’s acids, accompanied by subsequent heterocyclization, was investigated along with factors affecting the mixture composition of the obtained products. The plausible mechanism includes the formation of stable Michael adducts which, under the studied conditions, undergo further transformations to yield corresponding N-methylmorpholinium 4-aryl-6-oxo-3-(N-phenylthio-carbamoyl)-1,4,5,6-tetrahydropyridin-2-thiolates and their oxidation derivatives, 4,5-dihydro-3H-[1,2]dithiolo[3,4-b]pyridin-6(7H)-ones. The structure of one such product, N-methylmorpholinium 2,2-dimethyl-5-(1-(2-nitrophenyl)-3-(phenylamino)-2-(N-phenylthiocarbamoyl)-3-thioxopropyl)-4-oxo-4H-1,3-dioxin-6-olate, was confirmed via X-ray crystallography.
Gain or Loss? Evidence for Legume Predisposition to Symbiotic Interactions with Rhizobia via Loss of Pathogen-Resistance-Related Gene Families
Authors: Czyż Katarzyna B. , Taylor Candy M. , Kawaliło Michał , Koczyk Grzegorz
Published: December 2022

Nodulation is a hallmark yet non-universal characteristic of legumes. It is unknown whether the mechanisms underlying nitrogen-fixing symbioses evolved within legumes and the broader nitrogen-fixing clade (NFC) repeatedly de novo or based on common ancestral pathways. Ten new transcriptomes representing members from the Cercidoideae and Caesalpinioideae subfamilies were supplemented with published omics data from 65 angiosperms, to investigate how gene content correlates with nodulation capacity within Fabaceae and the NFC. Orthogroup analysis categorized annotated genes into 64150 orthogroups, of which 19 were significantly differentially represented between nodulating versus non-nodulating NFC species and were most commonly absent in nodulating taxa. The distribution of six over-represented orthogroups within Viridiplantae representatives suggested that genomic evolution events causing gene family expansions, including whole-genome duplications (WGDs), were unlikely to have facilitated the development of stable symbioses within Fabaceae as a whole. Instead, an absence of representation of 13 orthogroups indicated that losses of genes involved in trichome development, defense and wounding responses were strongly associated with rhizobial symbiosis in legumes. This finding provides novel evidence of a lineage-specific predisposition for the evolution and/or stabilization of nodulation in Fabaceae, in which a loss of pathogen resistance genes may have allowed for stable mutualistic interactions with rhizobia.
The Effect of Montmorillonites on the Physicochemical Properties of Potato Starch Films Plasticized with Deep Eutectic Solvent
Authors: Skowrońska Dorota , Wilpiszewska Katarzyna
Published: December 2022

In the paper, the method of obtaining the potato starch nanocomposites plasticized with a deep eutectic solvent is described. The deep eutectic solvent based on choline chloride and malic acid (CM, molar ratio 1:1) was used as the plasticizer. The effect of the sodium and calcium montmorillonite (MMTNa, MMTCa respectively) addition on the properties of potato starch films was investigated. The thermal, mechanical, and barrier properties were determined. Moreover, a moisture absorption test was performed. The starch gelatinization temperature increased in the presence of montmorillonite. The values of glass transition determined by DMTA depended on the nanofiller type. For the systems containing MMTCa, they generally decreased with its content (although still lower than reference samples). The obtained nanocomposites showed improved mechanical and barrier properties. The highest values of tensile strength and Young’s modulus were noted for the system containing 1% MMTNa. The XRD revealed that only the films with MMTNa exhibited intercalation. The homogeneity of the samples decreased with increasing nanofiller concentration. This was probably due to the occurrence of choline chloride-montmorillonite interactions, which were more favored than clay-starch interactions.
Can a Large Number of Transplanted Mesenchymal Stem Cells Have an Optimal Therapeutic Effect on Improving Ovarian Function?
Authors: Park Hyeri , Seok Jin , You Jun Hyeong , Lee Dae Hyun , Lim Ja-Yun , Kim Gi Jin
Published: December 2022

Mesenchymal stem cells (MSCs) are next-generation treatment in degenerative diseases. For the application of mesenchymal stem cell therapy to degenerative disease, transplantation conditions (e.g., optimized dose, delivery route and regenerating efficacy) should be considered. Recently, researchers have studied the mode of action of MSC in the treatment of ovarian degenerative disease. However, the evidence for the optimal number of cells for the developing stem cell therapeutics is insufficient. The objective of this study was to evaluate the efficacy in ovarian dysfunction, depends on cell dose. By intraovarian transplantation of low (1 × 105) and high (5 × 105) doses of placenta-derived mesenchymal stem cells (PD-MSCs) into thioacetamide (TAA)-injured rats, we compared the levels of apoptosis and oxidative stress that depend on different cell doses. Apoptosis and oxidative stress were significantly decreased in the transplanted (Tx) group compared to the non-transplanted (NTx) group in ovarian tissues from TAA-injured rats (* p < 0.05). In addition, we confirmed that follicular development was significantly increased in the Tx groups compared to the NTx group (* p < 0.05). However, there were no significant differences in the apoptosis, antioxidant or follicular development of injured ovarian tissues between the low and high doses PD-MSCs group. These findings provide new insights into the understanding and evidence obtained from clinical trials for stem cell therapy in reproductive systems.